doc_id	sent_index	relation_id	relation	trigger	trigger_offset	arg_num	arg_head	arg_protein	arg_domain	arg_site	arg_sugar	arg_head_offset	arg_base_np	arg_base_np_offset	arg_np	arg_np_offset	sent_text
29671580	2	0	theme	glycosylation	548:560	arg1	analysis					562:569	site-specific glycosylation analysis	534:569	site-specific glycosylation analysis using GPQuest software	534:592	In this study, we profiled serum N-glycoproteome by our recently developed N-glycoproteomic method using solid-phase extraction of N-linked glycans and glycosite-containing peptides (NGAG) coupled with LC-MS/MS and site-specific glycosylation analysis using GPQuest software.
29671580	1	1	gly	glycosylated	200:211	arg1	proteins					178:185	Most serum proteins	167:185	Most serum proteins	167:185	Most serum proteins are N-linked glycosylated, and therefore the glycoproteomic profiling of serum is essential for characterization of serum proteins.
29671580	2	2	theme	developed	384:392	arg1	method					411:416	our recently developed N-glycoproteomic method	371:416	our recently developed N-glycoproteomic method using solid-phase extraction of N-linked glycans and glycosite-containing peptides (NGAG) coupled with LC-MS/MS and site-specific glycosylation analysis using GPQuest software	371:592	In this study, we profiled serum N-glycoproteome by our recently developed N-glycoproteomic method using solid-phase extraction of N-linked glycans and glycosite-containing peptides (NGAG) coupled with LC-MS/MS and site-specific glycosylation analysis using GPQuest software.
29671580	2	3	theme	site-specific	534:546	arg1	analysis					562:569	site-specific glycosylation analysis	534:569	site-specific glycosylation analysis using GPQuest software	534:592	In this study, we profiled serum N-glycoproteome by our recently developed N-glycoproteomic method using solid-phase extraction of N-linked glycans and glycosite-containing peptides (NGAG) coupled with LC-MS/MS and site-specific glycosylation analysis using GPQuest software.
29671580	0	4	theme	N-Glycosylation	111:125	arg1	Sites					127:131	Atypical N-Glycosylation Sites	102:131	Atypical N-Glycosylation Sites on Albumin and α-1B-Glycoprotein	102:164	Site-Specific Profiling of Serum Glycoproteins Using N-Linked Glycan and Glycosite Analysis Revealing Atypical N-Glycosylation Sites on Albumin and α-1B-Glycoprotein.
29671580	6	5	gly	glycosites	1042:1051	arg2	glycosites					1042:1051	these two glycosites	1032:1051	these two glycosites	1032:1051	The widespread presence of these two glycosites among individuals was further confirmed by individual serum analyses.
29671580	3	6	theme	identified	627:636	arg1	N-glycosites					638:649	identified N-glycosites	627:649	identified N-glycosites	627:649	Our data indicated that half of identified N-glycosites were modified by at least two glycans, with a majority of them being sialylated.
29671580	0	7	theme	Atypical	102:109	arg1	Sites					127:131	Atypical N-Glycosylation Sites	102:131	Atypical N-Glycosylation Sites on Albumin and α-1B-Glycoprotein	102:164	Site-Specific Profiling of Serum Glycoproteins Using N-Linked Glycan and Glycosite Analysis Revealing Atypical N-Glycosylation Sites on Albumin and α-1B-Glycoprotein.
29671580	4	8	theme	glycosites	814:823	arg1	1/3					807:809	1/3	807:809	1/3	807:809	Specifically, 3/4 of glycosites were modified by biantennary N-glycans and 1/3 of glycosites were modified by triantennary sialylated N-glycans.
29671580	4	8	theme	glycosites	814:823	arg1	glycosites					814:823	glycosites	814:823	glycosites	814:823	Specifically, 3/4 of glycosites were modified by biantennary N-glycans and 1/3 of glycosites were modified by triantennary sialylated N-glycans.
29671580	1	9	theme	serum	303:307	arg1	proteins					309:316	serum proteins	303:316	serum proteins	303:316	Most serum proteins are N-linked glycosylated, and therefore the glycoproteomic profiling of serum is essential for characterization of serum proteins.
29671580	2	10	theme	GPQuest	577:583	arg1	software					585:592	GPQuest software	577:592	GPQuest software	577:592	In this study, we profiled serum N-glycoproteome by our recently developed N-glycoproteomic method using solid-phase extraction of N-linked glycans and glycosite-containing peptides (NGAG) coupled with LC-MS/MS and site-specific glycosylation analysis using GPQuest software.
29671580	4	11	mod	modified	830:837	arg1	1/3					807:809	1/3	807:809	1/3	807:809	Specifically, 3/4 of glycosites were modified by biantennary N-glycans and 1/3 of glycosites were modified by triantennary sialylated N-glycans.
29671580	4	11	mod	modified	830:837	arg3	N-glycans					866:874	triantennary sialylated N-glycans	842:874	triantennary sialylated N-glycans	842:874	Specifically, 3/4 of glycosites were modified by biantennary N-glycans and 1/3 of glycosites were modified by triantennary sialylated N-glycans.
29671580	4	11	mod	modified	830:837	arg1	glycosites					814:823	glycosites	814:823	glycosites	814:823	Specifically, 3/4 of glycosites were modified by biantennary N-glycans and 1/3 of glycosites were modified by triantennary sialylated N-glycans.
29671580	4	12	theme	glycosites	753:762	arg1	glycosites					753:762	glycosites	753:762	glycosites	753:762	Specifically, 3/4 of glycosites were modified by biantennary N-glycans and 1/3 of glycosites were modified by triantennary sialylated N-glycans.
29671580	4	12	theme	glycosites	753:762	arg1	3/4					746:748	3/4	746:748	3/4	746:748	Specifically, 3/4 of glycosites were modified by biantennary N-glycans and 1/3 of glycosites were modified by triantennary sialylated N-glycans.
29671580	5	13	theme	N-X-V	926:930	arg1	motif					932:936	N-X-V motif	926:936	N-X-V motif	926:936	In addition, two novel atypical glycosites (with N-X-V motif) were identified and validated from albumin and α-1B-glycoprotein.
29671580	4	14	gly	glycosites	814:823	arg2	glycosites					814:823	glycosites	814:823	glycosites	814:823	Specifically, 3/4 of glycosites were modified by biantennary N-glycans and 1/3 of glycosites were modified by triantennary sialylated N-glycans.
29671580	3	15	theme	N-glycosites	638:649	arg1	half					619:622	half	619:622	half of identified N-glycosites	619:649	Our data indicated that half of identified N-glycosites were modified by at least two glycans, with a majority of them being sialylated.
29671580	2	16	theme	solid-phase	424:434	arg1	extraction					436:445	solid-phase extraction	424:445	solid-phase extraction of N-linked glycans and glycosite-containing peptides (NGAG) coupled with LC-MS/MS	424:528	In this study, we profiled serum N-glycoproteome by our recently developed N-glycoproteomic method using solid-phase extraction of N-linked glycans and glycosite-containing peptides (NGAG) coupled with LC-MS/MS and site-specific glycosylation analysis using GPQuest software.
29671580	0	17	theme	Site-Specific	0:12	arg1	Profiling					14:22	Site-Specific Profiling	0:22	Site-Specific Profiling of Serum Glycoproteins	0:45	Site-Specific Profiling of Serum Glycoproteins Using N-Linked Glycan and Glycosite Analysis Revealing Atypical N-Glycosylation Sites on Albumin and α-1B-Glycoprotein.
29671580	1	18	theme	proteins	309:316	arg1	characterization					283:298	characterization	283:298	characterization of serum proteins	283:316	Most serum proteins are N-linked glycosylated, and therefore the glycoproteomic profiling of serum is essential for characterization of serum proteins.
29671580	5	19	gly	glycosites	909:918	arg2	glycosites					909:918	two novel atypical glycosites	890:918	two novel atypical glycosites (with N-X-V motif)	890:937	In addition, two novel atypical glycosites (with N-X-V motif) were identified and validated from albumin and α-1B-glycoprotein.
29671580	3	20	mod	modified	656:663	arg1	half					619:622	half	619:622	half of identified N-glycosites	619:649	Our data indicated that half of identified N-glycosites were modified by at least two glycans, with a majority of them being sialylated.
29671580	3	20	mod	modified	656:663	arg3	glycans					681:687	at least two glycans	668:687	at least two glycans	668:687	Our data indicated that half of identified N-glycosites were modified by at least two glycans, with a majority of them being sialylated.
29671580	3	21	theme	them	709:712	arg1	majority					697:704	a majority	695:704	a majority of them	695:712	Our data indicated that half of identified N-glycosites were modified by at least two glycans, with a majority of them being sialylated.
29671580	0	22	theme	Serum	27:31	arg1	Glycoproteins					33:45	Serum Glycoproteins	27:45	Serum Glycoproteins	27:45	Site-Specific Profiling of Serum Glycoproteins Using N-Linked Glycan and Glycosite Analysis Revealing Atypical N-Glycosylation Sites on Albumin and α-1B-Glycoprotein.
29671580	2	23	theme	N-glycoproteomic	394:409	arg1	method					411:416	our recently developed N-glycoproteomic method	371:416	our recently developed N-glycoproteomic method using solid-phase extraction of N-linked glycans and glycosite-containing peptides (NGAG) coupled with LC-MS/MS and site-specific glycosylation analysis using GPQuest software	371:592	In this study, we profiled serum N-glycoproteome by our recently developed N-glycoproteomic method using solid-phase extraction of N-linked glycans and glycosite-containing peptides (NGAG) coupled with LC-MS/MS and site-specific glycosylation analysis using GPQuest software.
29671580	5	24	with	glycosites	909:918	arg1	motif					932:936	N-X-V motif	926:936	N-X-V motif	926:936	In addition, two novel atypical glycosites (with N-X-V motif) were identified and validated from albumin and α-1B-glycoprotein.
29671580	1	25	dep	glycosylated	200:211	arg1	N-linked					191:198	N-linked	191:198	N-linked	191:198	Most serum proteins are N-linked glycosylated, and therefore the glycoproteomic profiling of serum is essential for characterization of serum proteins.
29671580	2	26	theme	glycosite-containing	471:490	arg1	peptides					492:499	glycosite-containing peptides	471:499	glycosite-containing peptides	471:499	In this study, we profiled serum N-glycoproteome by our recently developed N-glycoproteomic method using solid-phase extraction of N-linked glycans and glycosite-containing peptides (NGAG) coupled with LC-MS/MS and site-specific glycosylation analysis using GPQuest software.
29671580	2	27	theme	peptides	492:499	arg1	extraction					436:445	solid-phase extraction	424:445	solid-phase extraction of N-linked glycans and glycosite-containing peptides (NGAG) coupled with LC-MS/MS	424:528	In this study, we profiled serum N-glycoproteome by our recently developed N-glycoproteomic method using solid-phase extraction of N-linked glycans and glycosite-containing peptides (NGAG) coupled with LC-MS/MS and site-specific glycosylation analysis using GPQuest software.
29671580	2	27	theme	peptides	492:499	arg1	analysis					562:569	site-specific glycosylation analysis	534:569	site-specific glycosylation analysis using GPQuest software	534:592	In this study, we profiled serum N-glycoproteome by our recently developed N-glycoproteomic method using solid-phase extraction of N-linked glycans and glycosite-containing peptides (NGAG) coupled with LC-MS/MS and site-specific glycosylation analysis using GPQuest software.
29671580	2	28	gly	glycosylation	548:560	arg1	NGAG					502:505	NGAG	502:505	NGAG	502:505	In this study, we profiled serum N-glycoproteome by our recently developed N-glycoproteomic method using solid-phase extraction of N-linked glycans and glycosite-containing peptides (NGAG) coupled with LC-MS/MS and site-specific glycosylation analysis using GPQuest software.
29671580	2	28	gly	glycosylation	548:560	arg1	glycans					459:465	N-linked glycans	450:465	N-linked glycans	450:465	In this study, we profiled serum N-glycoproteome by our recently developed N-glycoproteomic method using solid-phase extraction of N-linked glycans and glycosite-containing peptides (NGAG) coupled with LC-MS/MS and site-specific glycosylation analysis using GPQuest software.
29671580	2	28	gly	glycosylation	548:560	arg1	peptides					492:499	glycosite-containing peptides	471:499	glycosite-containing peptides	471:499	In this study, we profiled serum N-glycoproteome by our recently developed N-glycoproteomic method using solid-phase extraction of N-linked glycans and glycosite-containing peptides (NGAG) coupled with LC-MS/MS and site-specific glycosylation analysis using GPQuest software.
29671580	5	29	theme	atypical	900:907	arg1	glycosites					909:918	two novel atypical glycosites	890:918	two novel atypical glycosites (with N-X-V motif)	890:937	In addition, two novel atypical glycosites (with N-X-V motif) were identified and validated from albumin and α-1B-glycoprotein.
29671580	5	30	gly	α-1B-glycoprotein	986:1002	arg1	α-1B-glycoprotein					986:1002	α-1B-glycoprotein	986:1002	α-1B-glycoprotein	986:1002	In addition, two novel atypical glycosites (with N-X-V motif) were identified and validated from albumin and α-1B-glycoprotein.
29671580	1	31	theme	glycoproteomic	232:245	arg1	essential					269:277	essential	269:277	essential	269:277	Most serum proteins are N-linked glycosylated, and therefore the glycoproteomic profiling of serum is essential for characterization of serum proteins.
29671580	1	31	theme	glycoproteomic	232:245	arg1	profiling					247:255	the glycoproteomic profiling	228:255	the glycoproteomic profiling of serum	228:264	Most serum proteins are N-linked glycosylated, and therefore the glycoproteomic profiling of serum is essential for characterization of serum proteins.
29671580	2	32	theme	glycans	459:465	arg1	extraction					436:445	solid-phase extraction	424:445	solid-phase extraction of N-linked glycans and glycosite-containing peptides (NGAG) coupled with LC-MS/MS	424:528	In this study, we profiled serum N-glycoproteome by our recently developed N-glycoproteomic method using solid-phase extraction of N-linked glycans and glycosite-containing peptides (NGAG) coupled with LC-MS/MS and site-specific glycosylation analysis using GPQuest software.
29671580	2	32	theme	glycans	459:465	arg1	analysis					562:569	site-specific glycosylation analysis	534:569	site-specific glycosylation analysis using GPQuest software	534:592	In this study, we profiled serum N-glycoproteome by our recently developed N-glycoproteomic method using solid-phase extraction of N-linked glycans and glycosite-containing peptides (NGAG) coupled with LC-MS/MS and site-specific glycosylation analysis using GPQuest software.
29671580	0	33	theme	Glycoproteins	33:45	arg1	Profiling					14:22	Site-Specific Profiling	0:22	Site-Specific Profiling of Serum Glycoproteins	0:45	Site-Specific Profiling of Serum Glycoproteins Using N-Linked Glycan and Glycosite Analysis Revealing Atypical N-Glycosylation Sites on Albumin and α-1B-Glycoprotein.
29671580	4	34	gly	sialylated	855:864	arg1	N-glycans					866:874	triantennary sialylated N-glycans	842:874	triantennary sialylated N-glycans	842:874	Specifically, 3/4 of glycosites were modified by biantennary N-glycans and 1/3 of glycosites were modified by triantennary sialylated N-glycans.
29671580	2	35	theme	N-linked	450:457	arg1	NGAG					502:505	NGAG	502:505	NGAG	502:505	In this study, we profiled serum N-glycoproteome by our recently developed N-glycoproteomic method using solid-phase extraction of N-linked glycans and glycosite-containing peptides (NGAG) coupled with LC-MS/MS and site-specific glycosylation analysis using GPQuest software.
29671580	2	35	theme	N-linked	450:457	arg1	glycans					459:465	N-linked glycans	450:465	N-linked glycans	450:465	In this study, we profiled serum N-glycoproteome by our recently developed N-glycoproteomic method using solid-phase extraction of N-linked glycans and glycosite-containing peptides (NGAG) coupled with LC-MS/MS and site-specific glycosylation analysis using GPQuest software.
29671580	6	36	theme	widespread	1009:1018	arg1	presence					1020:1027	The widespread presence	1005:1027	The widespread presence of these two glycosites among individuals	1005:1069	The widespread presence of these two glycosites among individuals was further confirmed by individual serum analyses.
29671580	0	37	theme	N-Linked	53:60	arg1	Glycan					62:67	N-Linked Glycan	53:67	N-Linked Glycan	53:67	Site-Specific Profiling of Serum Glycoproteins Using N-Linked Glycan and Glycosite Analysis Revealing Atypical N-Glycosylation Sites on Albumin and α-1B-Glycoprotein.
29671580	2	38	link	N-linked	450:457	arg1	NGAG					502:505	NGAG	502:505	NGAG	502:505	In this study, we profiled serum N-glycoproteome by our recently developed N-glycoproteomic method using solid-phase extraction of N-linked glycans and glycosite-containing peptides (NGAG) coupled with LC-MS/MS and site-specific glycosylation analysis using GPQuest software.
29671580	2	38	link	N-linked	450:457	arg1	glycans					459:465	N-linked glycans	450:465	N-linked glycans	450:465	In this study, we profiled serum N-glycoproteome by our recently developed N-glycoproteomic method using solid-phase extraction of N-linked glycans and glycosite-containing peptides (NGAG) coupled with LC-MS/MS and site-specific glycosylation analysis using GPQuest software.
29671580	3	39	gly	N-glycosites	638:649	arg2	N-glycosites					638:649	identified N-glycosites	627:649	identified N-glycosites	627:649	Our data indicated that half of identified N-glycosites were modified by at least two glycans, with a majority of them being sialylated.
29671580	0	40	from	Sites	127:131	arg1	α-1B-Glycoprotein					148:164	α-1B-Glycoprotein	148:164	α-1B-Glycoprotein	148:164	Site-Specific Profiling of Serum Glycoproteins Using N-Linked Glycan and Glycosite Analysis Revealing Atypical N-Glycosylation Sites on Albumin and α-1B-Glycoprotein.
29671580	0	40	from	Sites	127:131	arg1	Albumin					136:142	Albumin	136:142	Albumin	136:142	Site-Specific Profiling of Serum Glycoproteins Using N-Linked Glycan and Glycosite Analysis Revealing Atypical N-Glycosylation Sites on Albumin and α-1B-Glycoprotein.
29671580	4	41	theme	sialylated	855:864	arg1	N-glycans					866:874	triantennary sialylated N-glycans	842:874	triantennary sialylated N-glycans	842:874	Specifically, 3/4 of glycosites were modified by biantennary N-glycans and 1/3 of glycosites were modified by triantennary sialylated N-glycans.
29671580	6	42	theme	individual	1096:1105	arg1	analyses					1113:1120	individual serum analyses	1096:1120	individual serum analyses	1096:1120	The widespread presence of these two glycosites among individuals was further confirmed by individual serum analyses.
29671580	4	43	theme	biantennary	781:791	arg1	N-glycans					793:801	biantennary N-glycans	781:801	biantennary N-glycans	781:801	Specifically, 3/4 of glycosites were modified by biantennary N-glycans and 1/3 of glycosites were modified by triantennary sialylated N-glycans.
29671580	6	44	theme	serum	1107:1111	arg1	analyses					1113:1120	individual serum analyses	1096:1120	individual serum analyses	1096:1120	The widespread presence of these two glycosites among individuals was further confirmed by individual serum analyses.
29671580	4	45	gly	glycosites	753:762	arg2	glycosites					753:762	glycosites	753:762	glycosites	753:762	Specifically, 3/4 of glycosites were modified by biantennary N-glycans and 1/3 of glycosites were modified by triantennary sialylated N-glycans.
29671580	4	46	theme	triantennary	842:853	arg1	N-glycans					866:874	triantennary sialylated N-glycans	842:874	triantennary sialylated N-glycans	842:874	Specifically, 3/4 of glycosites were modified by biantennary N-glycans and 1/3 of glycosites were modified by triantennary sialylated N-glycans.
29671580	2	47	theme	serum	346:350	arg1	N-glycoproteome					352:366	serum N-glycoproteome	346:366	serum N-glycoproteome	346:366	In this study, we profiled serum N-glycoproteome by our recently developed N-glycoproteomic method using solid-phase extraction of N-linked glycans and glycosite-containing peptides (NGAG) coupled with LC-MS/MS and site-specific glycosylation analysis using GPQuest software.
29671580	1	48	theme	serum	260:264	arg1	essential					269:277	essential	269:277	essential	269:277	Most serum proteins are N-linked glycosylated, and therefore the glycoproteomic profiling of serum is essential for characterization of serum proteins.
29671580	1	48	theme	serum	260:264	arg1	profiling					247:255	the glycoproteomic profiling	228:255	the glycoproteomic profiling of serum	228:264	Most serum proteins are N-linked glycosylated, and therefore the glycoproteomic profiling of serum is essential for characterization of serum proteins.
29671580	0	49	theme	Glycosite	73:81	arg1	Analysis					83:90	Glycosite Analysis	73:90	Glycosite Analysis	73:90	Site-Specific Profiling of Serum Glycoproteins Using N-Linked Glycan and Glycosite Analysis Revealing Atypical N-Glycosylation Sites on Albumin and α-1B-Glycoprotein.
29671580	4	50	mod	modified	769:776	arg3	N-glycans					793:801	biantennary N-glycans	781:801	biantennary N-glycans	781:801	Specifically, 3/4 of glycosites were modified by biantennary N-glycans and 1/3 of glycosites were modified by triantennary sialylated N-glycans.
29671580	4	50	mod	modified	769:776	arg1	glycosites					753:762	glycosites	753:762	glycosites	753:762	Specifically, 3/4 of glycosites were modified by biantennary N-glycans and 1/3 of glycosites were modified by triantennary sialylated N-glycans.
29671580	4	50	mod	modified	769:776	arg1	3/4					746:748	3/4	746:748	3/4	746:748	Specifically, 3/4 of glycosites were modified by biantennary N-glycans and 1/3 of glycosites were modified by triantennary sialylated N-glycans.
29671580	5	51	theme	novel	894:898	arg1	glycosites					909:918	two novel atypical glycosites	890:918	two novel atypical glycosites (with N-X-V motif)	890:937	In addition, two novel atypical glycosites (with N-X-V motif) were identified and validated from albumin and α-1B-glycoprotein.
29671580	1	52	theme	Most	167:170	arg1	proteins					178:185	Most serum proteins	167:185	Most serum proteins	167:185	Most serum proteins are N-linked glycosylated, and therefore the glycoproteomic profiling of serum is essential for characterization of serum proteins.
29671580	3	53	gly	sialylated	720:729	arg1	majority					697:704	a majority	695:704	a majority of them	695:712	Our data indicated that half of identified N-glycosites were modified by at least two glycans, with a majority of them being sialylated.
29671580	6	54	theme	glycosites	1042:1051	arg1	presence					1020:1027	The widespread presence	1005:1027	The widespread presence of these two glycosites among individuals	1005:1069	The widespread presence of these two glycosites among individuals was further confirmed by individual serum analyses.
29671580	1	55	theme	serum	172:176	arg1	proteins					178:185	Most serum proteins	167:185	Most serum proteins	167:185	Most serum proteins are N-linked glycosylated, and therefore the glycoproteomic profiling of serum is essential for characterization of serum proteins.
29736039	4	0	theme	unique	831:836	arg1	peptides					838:845	3,480 unique peptides	825:845	3,480 unique peptides	825:845	We produced six N- and O-linked polypeptide-modifying glycosyltransferases from bacteria and humans in vitro and rigorously determined their substrate specificities using 3,480 unique peptides and 13,903 unique reaction conditions.
29736039	3	1	theme	protein	584:590	arg1	synthesis					592:600	cell-free protein synthesis	574:600	cell-free protein synthesis	574:600	Here we describe a systematic platform for glycosylation sequence characterization and optimization by rapid expression and screening (GlycoSCORES), which combines cell-free protein synthesis and mass spectrometry of self-assembled monolayers.
29736039	0	2	theme	glycosyltransferases	65:84	arg1	analysis					53:60	analysis	53:60	analysis of glycosyltransferases	53:84	Design of glycosylation sites by rapid synthesis and analysis of glycosyltransferases.
29736039	0	2	theme	glycosyltransferases	65:84	arg1	synthesis					39:47	rapid synthesis	33:47	rapid synthesis	33:47	Design of glycosylation sites by rapid synthesis and analysis of glycosyltransferases.
29736039	5	3	used	used	894:897	arg2	We					886:887	We	886:887	We	886:887	We then used GlycoSCORES to optimize and design small glycosylation sequence motifs that directed efficient N-linked glycosylation in vitro and in the Escherichia coli cytoplasm for three heterologous proteins, including the human immunoglobulin Fc domain.
29736039	1	4	from	disease	169:175	arg1	Glycosylation					87:99	Glycosylation	87:99	Glycosylation	87:99	Glycosylation is an abundant post-translational modification that is important in disease and biotechnology.
29736039	1	4	from	disease	169:175	arg1	modification					135:146	an abundant post-translational modification	104:146	an abundant post-translational modification that is important in disease and biotechnology	104:193	Glycosylation is an abundant post-translational modification that is important in disease and biotechnology.
29736039	1	4	from	disease	169:175	arg1	important					156:164	important	156:164	important	156:164	Glycosylation is an abundant post-translational modification that is important in disease and biotechnology.
29736039	4	5	theme	N-	670:671	arg1	glycosyltransferases					708:727	six N- and O-linked polypeptide-modifying glycosyltransferases	666:727	six N- and O-linked polypeptide-modifying glycosyltransferases from bacteria and humans	666:752	We produced six N- and O-linked polypeptide-modifying glycosyltransferases from bacteria and humans in vitro and rigorously determined their substrate specificities using 3,480 unique peptides and 13,903 unique reaction conditions.
29736039	5	6	dep	Escherichia	1037:1047	arg1	coli					1049:1052	coli	1049:1052	coli	1049:1052	We then used GlycoSCORES to optimize and design small glycosylation sequence motifs that directed efficient N-linked glycosylation in vitro and in the Escherichia coli cytoplasm for three heterologous proteins, including the human immunoglobulin Fc domain.
29736039	3	7	theme	self-assembled	627:640	arg1	monolayers					642:651	self-assembled monolayers	627:651	self-assembled monolayers	627:651	Here we describe a systematic platform for glycosylation sequence characterization and optimization by rapid expression and screening (GlycoSCORES), which combines cell-free protein synthesis and mass spectrometry of self-assembled monolayers.
29736039	5	8	theme	human	1111:1115	arg1	domain					1135:1140	the human immunoglobulin Fc domain	1107:1140	the human immunoglobulin Fc domain	1107:1140	We then used GlycoSCORES to optimize and design small glycosylation sequence motifs that directed efficient N-linked glycosylation in vitro and in the Escherichia coli cytoplasm for three heterologous proteins, including the human immunoglobulin Fc domain.
29736039	4	9	theme	polypeptide-modifying	686:706	arg1	glycosyltransferases					708:727	six N- and O-linked polypeptide-modifying glycosyltransferases	666:727	six N- and O-linked polypeptide-modifying glycosyltransferases from bacteria and humans	666:752	We produced six N- and O-linked polypeptide-modifying glycosyltransferases from bacteria and humans in vitro and rigorously determined their substrate specificities using 3,480 unique peptides and 13,903 unique reaction conditions.
29736039	5	10	theme	Fc	1132:1133	arg1	domain					1135:1140	the human immunoglobulin Fc domain	1107:1140	the human immunoglobulin Fc domain	1107:1140	We then used GlycoSCORES to optimize and design small glycosylation sequence motifs that directed efficient N-linked glycosylation in vitro and in the Escherichia coli cytoplasm for three heterologous proteins, including the human immunoglobulin Fc domain.
29736039	1	11	from	important	156:164	arg1	biotechnology					181:193	biotechnology	181:193	biotechnology	181:193	Glycosylation is an abundant post-translational modification that is important in disease and biotechnology.
29736039	1	11	from	important	156:164	arg1	disease					169:175	disease	169:175	disease	169:175	Glycosylation is an abundant post-translational modification that is important in disease and biotechnology.
29736039	3	12	theme	monolayers	642:651	arg1	synthesis					592:600	cell-free protein synthesis	574:600	cell-free protein synthesis	574:600	Here we describe a systematic platform for glycosylation sequence characterization and optimization by rapid expression and screening (GlycoSCORES), which combines cell-free protein synthesis and mass spectrometry of self-assembled monolayers.
29736039	3	12	theme	monolayers	642:651	arg1	spectrometry					611:622	mass spectrometry	606:622	mass spectrometry	606:622	Here we describe a systematic platform for glycosylation sequence characterization and optimization by rapid expression and screening (GlycoSCORES), which combines cell-free protein synthesis and mass spectrometry of self-assembled monolayers.
29736039	4	13	theme	O-linked	677:684	arg1	glycosyltransferases					708:727	six N- and O-linked polypeptide-modifying glycosyltransferases	666:727	six N- and O-linked polypeptide-modifying glycosyltransferases from bacteria and humans	666:752	We produced six N- and O-linked polypeptide-modifying glycosyltransferases from bacteria and humans in vitro and rigorously determined their substrate specificities using 3,480 unique peptides and 13,903 unique reaction conditions.
29736039	6	14	theme	synthetic	1276:1284	arg1	glycoproteins					1286:1298	synthetic glycoproteins	1276:1298	synthetic glycoproteins	1276:1298	We find that GlycoSCORES is a broadly applicable method to facilitate fundamental understanding of glycosyltransferases and engineer synthetic glycoproteins.
29736039	5	15	theme	sequence	954:961	arg1	motifs					963:968	small glycosylation sequence motifs	934:968	small glycosylation sequence motifs that directed efficient N-linked glycosylation in vitro and in the Escherichia coli cytoplasm for three heterologous proteins, including the human immunoglobulin Fc domain	934:1140	We then used GlycoSCORES to optimize and design small glycosylation sequence motifs that directed efficient N-linked glycosylation in vitro and in the Escherichia coli cytoplasm for three heterologous proteins, including the human immunoglobulin Fc domain.
29736039	6	16	theme	glycosyltransferases	1242:1261	arg1	understanding					1225:1237	fundamental understanding	1213:1237	fundamental understanding of glycosyltransferases	1213:1261	We find that GlycoSCORES is a broadly applicable method to facilitate fundamental understanding of glycosyltransferases and engineer synthetic glycoproteins.
29736039	5	17	theme	glycosylation	940:952	arg1	motifs					963:968	small glycosylation sequence motifs	934:968	small glycosylation sequence motifs that directed efficient N-linked glycosylation in vitro and in the Escherichia coli cytoplasm for three heterologous proteins, including the human immunoglobulin Fc domain	934:1140	We then used GlycoSCORES to optimize and design small glycosylation sequence motifs that directed efficient N-linked glycosylation in vitro and in the Escherichia coli cytoplasm for three heterologous proteins, including the human immunoglobulin Fc domain.
29736039	5	18	gly	glycosylation	1003:1015	arg1	vitro					1020:1024	vitro	1020:1024	vitro	1020:1024	We then used GlycoSCORES to optimize and design small glycosylation sequence motifs that directed efficient N-linked glycosylation in vitro and in the Escherichia coli cytoplasm for three heterologous proteins, including the human immunoglobulin Fc domain.
29736039	4	19	theme	substrate	795:803	arg1	specificities					805:817	their substrate specificities	789:817	their substrate specificities	789:817	We produced six N- and O-linked polypeptide-modifying glycosyltransferases from bacteria and humans in vitro and rigorously determined their substrate specificities using 3,480 unique peptides and 13,903 unique reaction conditions.
29736039	3	20	theme	glycosylation	453:465	arg1	characterization					476:491	glycosylation sequence characterization	453:491	glycosylation sequence characterization	453:491	Here we describe a systematic platform for glycosylation sequence characterization and optimization by rapid expression and screening (GlycoSCORES), which combines cell-free protein synthesis and mass spectrometry of self-assembled monolayers.
29736039	2	21	theme	glycosylation	356:368	arg1	sites					370:374	glycosylation sites	356:374	glycosylation sites modified by glycosyltransferases	356:407	Current methods to understand and engineer glycosylation cannot sufficiently explore the vast experimental landscapes required to accurately predict and design glycosylation sites modified by glycosyltransferases.
29736039	3	22	theme	rapid	513:517	arg1	expression					519:528	rapid expression	513:528	rapid expression	513:528	Here we describe a systematic platform for glycosylation sequence characterization and optimization by rapid expression and screening (GlycoSCORES), which combines cell-free protein synthesis and mass spectrometry of self-assembled monolayers.
29736039	3	23	theme	systematic	429:438	arg1	platform					440:447	a systematic platform	427:447	a systematic platform	427:447	Here we describe a systematic platform for glycosylation sequence characterization and optimization by rapid expression and screening (GlycoSCORES), which combines cell-free protein synthesis and mass spectrometry of self-assembled monolayers.
29736039	4	24	theme	reaction	865:872	arg1	conditions					874:883	13,903 unique reaction conditions	851:883	13,903 unique reaction conditions	851:883	We produced six N- and O-linked polypeptide-modifying glycosyltransferases from bacteria and humans in vitro and rigorously determined their substrate specificities using 3,480 unique peptides and 13,903 unique reaction conditions.
29736039	5	25	theme	immunoglobulin	1117:1130	arg1	domain					1135:1140	the human immunoglobulin Fc domain	1107:1140	the human immunoglobulin Fc domain	1107:1140	We then used GlycoSCORES to optimize and design small glycosylation sequence motifs that directed efficient N-linked glycosylation in vitro and in the Escherichia coli cytoplasm for three heterologous proteins, including the human immunoglobulin Fc domain.
29736039	0	26	theme	glycosylation	10:22	arg1	sites					24:28	glycosylation sites	10:28	glycosylation sites	10:28	Design of glycosylation sites by rapid synthesis and analysis of glycosyltransferases.
29736039	4	27	theme	six	666:668	arg1	N-					670:671	N-	670:671	N-	670:671	We produced six N- and O-linked polypeptide-modifying glycosyltransferases from bacteria and humans in vitro and rigorously determined their substrate specificities using 3,480 unique peptides and 13,903 unique reaction conditions.
29736039	1	28	theme	abundant	107:114	arg1	important					156:164	important	156:164	important	156:164	Glycosylation is an abundant post-translational modification that is important in disease and biotechnology.
29736039	1	28	theme	abundant	107:114	arg1	modification					135:146	an abundant post-translational modification	104:146	an abundant post-translational modification that is important in disease and biotechnology	104:193	Glycosylation is an abundant post-translational modification that is important in disease and biotechnology.
29736039	1	28	theme	abundant	107:114	arg1	Glycosylation					87:99	Glycosylation	87:99	Glycosylation	87:99	Glycosylation is an abundant post-translational modification that is important in disease and biotechnology.
29736039	5	29	theme	efficient	984:992	arg1	glycosylation					1003:1015	efficient N-linked glycosylation	984:1015	efficient N-linked glycosylation	984:1015	We then used GlycoSCORES to optimize and design small glycosylation sequence motifs that directed efficient N-linked glycosylation in vitro and in the Escherichia coli cytoplasm for three heterologous proteins, including the human immunoglobulin Fc domain.
29736039	0	30	theme	rapid	33:37	arg1	synthesis					39:47	rapid synthesis	33:47	rapid synthesis	33:47	Design of glycosylation sites by rapid synthesis and analysis of glycosyltransferases.
29736039	2	31	theme	Current	196:202	arg1	methods					204:210	Current methods	196:210	Current methods	196:210	Current methods to understand and engineer glycosylation cannot sufficiently explore the vast experimental landscapes required to accurately predict and design glycosylation sites modified by glycosyltransferases.
29736039	4	32	from	humans	747:752	arg1	glycosyltransferases					708:727	six N- and O-linked polypeptide-modifying glycosyltransferases	666:727	six N- and O-linked polypeptide-modifying glycosyltransferases from bacteria and humans	666:752	We produced six N- and O-linked polypeptide-modifying glycosyltransferases from bacteria and humans in vitro and rigorously determined their substrate specificities using 3,480 unique peptides and 13,903 unique reaction conditions.
29736039	5	33	theme	N-linked	994:1001	arg1	glycosylation					1003:1015	efficient N-linked glycosylation	984:1015	efficient N-linked glycosylation	984:1015	We then used GlycoSCORES to optimize and design small glycosylation sequence motifs that directed efficient N-linked glycosylation in vitro and in the Escherichia coli cytoplasm for three heterologous proteins, including the human immunoglobulin Fc domain.
29736039	0	34	theme	sites	24:28	arg1	Design					0:5	Design	0:5	Design of glycosylation sites by rapid synthesis and analysis of glycosyltransferases.	0:85	Design of glycosylation sites by rapid synthesis and analysis of glycosyltransferases.
29736039	3	35	theme	sequence	467:474	arg1	characterization					476:491	glycosylation sequence characterization	453:491	glycosylation sequence characterization	453:491	Here we describe a systematic platform for glycosylation sequence characterization and optimization by rapid expression and screening (GlycoSCORES), which combines cell-free protein synthesis and mass spectrometry of self-assembled monolayers.
29736039	0	36	gly	glycosylation	10:22	arg2	sites					24:28	glycosylation sites	10:28	glycosylation sites	10:28	Design of glycosylation sites by rapid synthesis and analysis of glycosyltransferases.
29736039	1	37	theme	post-translational	116:133	arg1	important					156:164	important	156:164	important	156:164	Glycosylation is an abundant post-translational modification that is important in disease and biotechnology.
29736039	1	37	theme	post-translational	116:133	arg1	modification					135:146	an abundant post-translational modification	104:146	an abundant post-translational modification that is important in disease and biotechnology	104:193	Glycosylation is an abundant post-translational modification that is important in disease and biotechnology.
29736039	1	37	theme	post-translational	116:133	arg1	Glycosylation					87:99	Glycosylation	87:99	Glycosylation	87:99	Glycosylation is an abundant post-translational modification that is important in disease and biotechnology.
29736039	5	38	theme	small	934:938	arg1	motifs					963:968	small glycosylation sequence motifs	934:968	small glycosylation sequence motifs that directed efficient N-linked glycosylation in vitro and in the Escherichia coli cytoplasm for three heterologous proteins, including the human immunoglobulin Fc domain	934:1140	We then used GlycoSCORES to optimize and design small glycosylation sequence motifs that directed efficient N-linked glycosylation in vitro and in the Escherichia coli cytoplasm for three heterologous proteins, including the human immunoglobulin Fc domain.
29736039	3	39	theme	cell-free	574:582	arg1	synthesis					592:600	cell-free protein synthesis	574:600	cell-free protein synthesis	574:600	Here we describe a systematic platform for glycosylation sequence characterization and optimization by rapid expression and screening (GlycoSCORES), which combines cell-free protein synthesis and mass spectrometry of self-assembled monolayers.
29736039	4	40	link	O-linked	677:684	arg1	glycosyltransferases					708:727	six N- and O-linked polypeptide-modifying glycosyltransferases	666:727	six N- and O-linked polypeptide-modifying glycosyltransferases from bacteria and humans	666:752	We produced six N- and O-linked polypeptide-modifying glycosyltransferases from bacteria and humans in vitro and rigorously determined their substrate specificities using 3,480 unique peptides and 13,903 unique reaction conditions.
29736039	5	41	gly	glycosylation	940:952	arg2	motifs					963:968	small glycosylation sequence motifs	934:968	small glycosylation sequence motifs that directed efficient N-linked glycosylation in vitro and in the Escherichia coli cytoplasm for three heterologous proteins, including the human immunoglobulin Fc domain	934:1140	We then used GlycoSCORES to optimize and design small glycosylation sequence motifs that directed efficient N-linked glycosylation in vitro and in the Escherichia coli cytoplasm for three heterologous proteins, including the human immunoglobulin Fc domain.
29736039	4	42	theme	unique	858:863	arg1	conditions					874:883	13,903 unique reaction conditions	851:883	13,903 unique reaction conditions	851:883	We produced six N- and O-linked polypeptide-modifying glycosyltransferases from bacteria and humans in vitro and rigorously determined their substrate specificities using 3,480 unique peptides and 13,903 unique reaction conditions.
29736039	6	43	theme	applicable	1181:1190	arg1	method					1192:1197	a broadly applicable method	1171:1197	a broadly applicable method to facilitate fundamental understanding of glycosyltransferases and engineer synthetic glycoproteins	1171:1298	We find that GlycoSCORES is a broadly applicable method to facilitate fundamental understanding of glycosyltransferases and engineer synthetic glycoproteins.
29736039	6	43	theme	applicable	1181:1190	arg1	GlycoSCORES					1156:1166	GlycoSCORES	1156:1166	GlycoSCORES	1156:1166	We find that GlycoSCORES is a broadly applicable method to facilitate fundamental understanding of glycosyltransferases and engineer synthetic glycoproteins.
29736039	2	44	theme	experimental	290:301	arg1	landscapes					303:312	the vast experimental landscapes	281:312	the vast experimental landscapes required to accurately predict and design glycosylation sites modified by glycosyltransferases	281:407	Current methods to understand and engineer glycosylation cannot sufficiently explore the vast experimental landscapes required to accurately predict and design glycosylation sites modified by glycosyltransferases.
29736039	5	45	theme	Escherichia	1037:1047	arg1	cytoplasm					1054:1062	the Escherichia coli cytoplasm	1033:1062	the Escherichia coli cytoplasm for three heterologous proteins, including the human immunoglobulin Fc domain	1033:1140	We then used GlycoSCORES to optimize and design small glycosylation sequence motifs that directed efficient N-linked glycosylation in vitro and in the Escherichia coli cytoplasm for three heterologous proteins, including the human immunoglobulin Fc domain.
29736039	5	46	theme	heterologous	1074:1085	arg1	domain					1135:1140	the human immunoglobulin Fc domain	1107:1140	the human immunoglobulin Fc domain	1107:1140	We then used GlycoSCORES to optimize and design small glycosylation sequence motifs that directed efficient N-linked glycosylation in vitro and in the Escherichia coli cytoplasm for three heterologous proteins, including the human immunoglobulin Fc domain.
29736039	5	46	theme	heterologous	1074:1085	arg1	proteins					1087:1094	three heterologous proteins	1068:1094	three heterologous proteins	1068:1094	We then used GlycoSCORES to optimize and design small glycosylation sequence motifs that directed efficient N-linked glycosylation in vitro and in the Escherichia coli cytoplasm for three heterologous proteins, including the human immunoglobulin Fc domain.
29736039	2	47	gly	glycosylation	356:368	arg2	sites					370:374	glycosylation sites	356:374	glycosylation sites modified by glycosyltransferases	356:407	Current methods to understand and engineer glycosylation cannot sufficiently explore the vast experimental landscapes required to accurately predict and design glycosylation sites modified by glycosyltransferases.
29736039	2	48	theme	vast	285:288	arg1	landscapes					303:312	the vast experimental landscapes	281:312	the vast experimental landscapes required to accurately predict and design glycosylation sites modified by glycosyltransferases	281:407	Current methods to understand and engineer glycosylation cannot sufficiently explore the vast experimental landscapes required to accurately predict and design glycosylation sites modified by glycosyltransferases.
29736039	6	49	theme	fundamental	1213:1223	arg1	understanding					1225:1237	fundamental understanding	1213:1237	fundamental understanding of glycosyltransferases	1213:1261	We find that GlycoSCORES is a broadly applicable method to facilitate fundamental understanding of glycosyltransferases and engineer synthetic glycoproteins.
29736039	6	50	gly	glycoproteins	1286:1298	arg1	glycoproteins					1286:1298	synthetic glycoproteins	1276:1298	synthetic glycoproteins	1276:1298	We find that GlycoSCORES is a broadly applicable method to facilitate fundamental understanding of glycosyltransferases and engineer synthetic glycoproteins.
29736039	4	51	from	bacteria	734:741	arg1	glycosyltransferases					708:727	six N- and O-linked polypeptide-modifying glycosyltransferases	666:727	six N- and O-linked polypeptide-modifying glycosyltransferases from bacteria and humans	666:752	We produced six N- and O-linked polypeptide-modifying glycosyltransferases from bacteria and humans in vitro and rigorously determined their substrate specificities using 3,480 unique peptides and 13,903 unique reaction conditions.
29736039	1	52	from	biotechnology	181:193	arg1	Glycosylation					87:99	Glycosylation	87:99	Glycosylation	87:99	Glycosylation is an abundant post-translational modification that is important in disease and biotechnology.
29736039	1	52	from	biotechnology	181:193	arg1	modification					135:146	an abundant post-translational modification	104:146	an abundant post-translational modification that is important in disease and biotechnology	104:193	Glycosylation is an abundant post-translational modification that is important in disease and biotechnology.
29736039	1	52	from	biotechnology	181:193	arg1	important					156:164	important	156:164	important	156:164	Glycosylation is an abundant post-translational modification that is important in disease and biotechnology.
29736039	3	53	theme	mass	606:609	arg1	spectrometry					611:622	mass spectrometry	606:622	mass spectrometry	606:622	Here we describe a systematic platform for glycosylation sequence characterization and optimization by rapid expression and screening (GlycoSCORES), which combines cell-free protein synthesis and mass spectrometry of self-assembled monolayers.
29736039	5	54	link	N-linked	994:1001	arg1	glycosylation					1003:1015	efficient N-linked glycosylation	984:1015	efficient N-linked glycosylation	984:1015	We then used GlycoSCORES to optimize and design small glycosylation sequence motifs that directed efficient N-linked glycosylation in vitro and in the Escherichia coli cytoplasm for three heterologous proteins, including the human immunoglobulin Fc domain.
29604477	7	0	theme	subviral	1083:1090	arg1	particles					1092:1100	subviral particles	1083:1100	subviral particles	1083:1100	Impaired virion secretion was not necessarily associated with a similar block in the secretion of subviral particles.
29604477	0	1	theme	protein	158:164	arg1	Role					131:134	Role	131:134	Impact of immune escape mutations and N-linked glycosylation on the secretion of hepatitis B virus virions and subviral particles: Role of the small envelope protein.	0:165	Impact of immune escape mutations and N-linked glycosylation on the secretion of hepatitis B virus virions and subviral particles: Role of the small envelope protein.
29604477	7	2	from	block	1057:1061	arg1	secretion					1070:1078	the secretion	1066:1078	the secretion of subviral particles	1066:1100	Impaired virion secretion was not necessarily associated with a similar block in the secretion of subviral particles.
29604477	1	3	theme	envelope	219:226	arg1	proteins					228:235	three co-terminal envelope proteins	201:235	three co-terminal envelope proteins: large (L), middle (M), and small (S)	201:273	Hepatitis B virus (HBV) expresses three co-terminal envelope proteins: large (L), middle (M), and small (S), with the S protein driving the secretion of both virions and subviral particles.
29604477	2	4	theme	N-linked	383:390	arg1	glycosylation					392:404	N-linked glycosylation	383:404	N-linked glycosylation	383:404	Virion secretion requires N-linked glycosylation at N146 in the S domain but can be impaired by immune escape mutations.
29604477	3	5	theme	mutant	578:583	arg1	secretion					559:567	virion secretion	552:567	virion secretion of N146Q mutant (loss of original glycosylation site) and immune escape mutants such as G145R	552:661	An M133T mutation creating a novel glycosylation site at N131could rescue virion secretion of N146Q mutant (loss of original glycosylation site) and immune escape mutants such as G145R.
29604477	0	6	theme	envelope	149:156	arg1	protein					158:164	the small envelope protein	139:164	the small envelope protein	139:164	Impact of immune escape mutations and N-linked glycosylation on the secretion of hepatitis B virus virions and subviral particles: Role of the small envelope protein.
29604477	1	7	theme	virions	325:331	arg1	secretion					307:315	the secretion	303:315	the secretion of both virions and subviral particles	303:354	Hepatitis B virus (HBV) expresses three co-terminal envelope proteins: large (L), middle (M), and small (S), with the S protein driving the secretion of both virions and subviral particles.
29604477	7	8	theme	Impaired	985:992	arg1	secretion					1001:1009	Impaired virion secretion	985:1009	Impaired virion secretion	985:1009	Impaired virion secretion was not necessarily associated with a similar block in the secretion of subviral particles.
29604477	7	9	theme	virion	994:999	arg1	secretion					1001:1009	Impaired virion secretion	985:1009	Impaired virion secretion	985:1009	Impaired virion secretion was not necessarily associated with a similar block in the secretion of subviral particles.
29604477	0	10	theme	B	91:91	arg1	virus					93:97	hepatitis B virus virions and subviral particles	81:128	virus	93:97	Impact of immune escape mutations and N-linked glycosylation on the secretion of hepatitis B virus virions and subviral particles: Role of the small envelope protein.
29604477	0	11	from	Impact	0:5	arg1	secretion					68:76	the secretion	64:76	the secretion of hepatitis B virus virions and subviral particles	64:128	Impact of immune escape mutations and N-linked glycosylation on the secretion of hepatitis B virus virions and subviral particles: Role of the small envelope protein.
29604477	4	12	theme	G145R	767:771	arg1	secretion					750:758	virion secretion	743:758	virion secretion of the G145R and N146Q mutants to variable extents	743:809	Here we demonstrate that other novel N-linked glycosylation sites could rescue virion secretion of the G145R and N146Q mutants to variable extents.
29604477	4	13	theme	glycosylation	710:722	arg1	sites					724:728	other novel N-linked glycosylation sites	689:728	other novel N-linked glycosylation sites	689:728	Here we demonstrate that other novel N-linked glycosylation sites could rescue virion secretion of the G145R and N146Q mutants to variable extents.
29604477	5	14	theme	S	881:881	arg1	protein					883:889	the S protein	877:889	the S protein	877:889	Both G145R and N146Q mutations impaired virion secretion through the S protein.
29604477	0	15	theme	hepatitis	81:89	arg1	virus					93:97	hepatitis B virus virions and subviral particles	81:128	virus	93:97	Impact of immune escape mutations and N-linked glycosylation on the secretion of hepatitis B virus virions and subviral particles: Role of the small envelope protein.
29604477	3	16	theme	site	617:620	arg1	loss					586:589	loss	586:589	loss of original glycosylation site	586:620	An M133T mutation creating a novel glycosylation site at N131could rescue virion secretion of N146Q mutant (loss of original glycosylation site) and immune escape mutants such as G145R.
29604477	3	17	dep	mutant	578:583	arg1	loss					586:589	loss	586:589	loss of original glycosylation site	586:620	An M133T mutation creating a novel glycosylation site at N131could rescue virion secretion of N146Q mutant (loss of original glycosylation site) and immune escape mutants such as G145R.
29604477	1	18	theme	subviral	337:344	arg1	particles					346:354	subviral particles	337:354	subviral particles	337:354	Hepatitis B virus (HBV) expresses three co-terminal envelope proteins: large (L), middle (M), and small (S), with the S protein driving the secretion of both virions and subviral particles.
29604477	0	19	dep	virus	93:97	arg1	virions					99:105	virions	99:105	virions	99:105	Impact of immune escape mutations and N-linked glycosylation on the secretion of hepatitis B virus virions and subviral particles: Role of the small envelope protein.
29604477	3	20	from	N131could	535:543	arg1	site					527:530	a novel glycosylation site	505:530	a novel glycosylation site at N131could	505:543	An M133T mutation creating a novel glycosylation site at N131could rescue virion secretion of N146Q mutant (loss of original glycosylation site) and immune escape mutants such as G145R.
29604477	1	21	theme	particles	346:354	arg1	secretion					307:315	the secretion	303:315	the secretion of both virions and subviral particles	303:354	Hepatitis B virus (HBV) expresses three co-terminal envelope proteins: large (L), middle (M), and small (S), with the S protein driving the secretion of both virions and subviral particles.
29604477	2	22	theme	immune	453:458	arg1	mutations					467:475	immune escape mutations	453:475	immune escape mutations	453:475	Virion secretion requires N-linked glycosylation at N146 in the S domain but can be impaired by immune escape mutations.
29604477	3	23	gly	glycosylation	513:525	arg2	site					527:530	a novel glycosylation site	505:530	a novel glycosylation site at N131could	505:543	An M133T mutation creating a novel glycosylation site at N131could rescue virion secretion of N146Q mutant (loss of original glycosylation site) and immune escape mutants such as G145R.
29604477	3	23	gly	glycosylation	513:525	arg2	N131could					535:543	N131could	535:543	N131could	535:543	An M133T mutation creating a novel glycosylation site at N131could rescue virion secretion of N146Q mutant (loss of original glycosylation site) and immune escape mutants such as G145R.
29604477	0	24	theme	virus	93:97	arg1	secretion					68:76	the secretion	64:76	the secretion of hepatitis B virus virions and subviral particles	64:128	Impact of immune escape mutations and N-linked glycosylation on the secretion of hepatitis B virus virions and subviral particles: Role of the small envelope protein.
29604477	3	25	theme	original	594:601	arg1	site					617:620	original glycosylation site	594:620	original glycosylation site	594:620	An M133T mutation creating a novel glycosylation site at N131could rescue virion secretion of N146Q mutant (loss of original glycosylation site) and immune escape mutants such as G145R.
29604477	4	26	gly	glycosylation	710:722	arg2	sites					724:728	other novel N-linked glycosylation sites	689:728	other novel N-linked glycosylation sites	689:728	Here we demonstrate that other novel N-linked glycosylation sites could rescue virion secretion of the G145R and N146Q mutants to variable extents.
29604477	4	27	link	N-linked	701:708	arg1	sites					724:728	other novel N-linked glycosylation sites	689:728	other novel N-linked glycosylation sites	689:728	Here we demonstrate that other novel N-linked glycosylation sites could rescue virion secretion of the G145R and N146Q mutants to variable extents.
29604477	7	28	theme	similar	1049:1055	arg1	block					1057:1061	a similar block	1047:1061	a similar block in the secretion of subviral particles	1047:1100	Impaired virion secretion was not necessarily associated with a similar block in the secretion of subviral particles.
29604477	3	29	theme	glycosylation	603:615	arg1	site					617:620	original glycosylation site	594:620	original glycosylation site	594:620	An M133T mutation creating a novel glycosylation site at N131could rescue virion secretion of N146Q mutant (loss of original glycosylation site) and immune escape mutants such as G145R.
29604477	4	30	theme	virion	743:748	arg1	secretion					750:758	virion secretion	743:758	virion secretion of the G145R and N146Q mutants to variable extents	743:809	Here we demonstrate that other novel N-linked glycosylation sites could rescue virion secretion of the G145R and N146Q mutants to variable extents.
29604477	0	31	link	N-linked	38:45	arg1	glycosylation					47:59	N-linked glycosylation	38:59	N-linked glycosylation	38:59	Impact of immune escape mutations and N-linked glycosylation on the secretion of hepatitis B virus virions and subviral particles: Role of the small envelope protein.
29604477	5	32	theme	G145R	817:821	arg1	mutations					833:841	Both G145R and N146Q mutations	812:841	Both G145R and N146Q mutations	812:841	Both G145R and N146Q mutations impaired virion secretion through the S protein.
29604477	3	33	theme	escape	634:639	arg1	mutants					641:647	immune escape mutants	627:647	immune escape mutants such as G145R	627:661	An M133T mutation creating a novel glycosylation site at N131could rescue virion secretion of N146Q mutant (loss of original glycosylation site) and immune escape mutants such as G145R.
29604477	3	33	theme	escape	634:639	arg1	G145R					657:661	G145R	657:661	G145R	657:661	An M133T mutation creating a novel glycosylation site at N131could rescue virion secretion of N146Q mutant (loss of original glycosylation site) and immune escape mutants such as G145R.
29604477	1	34	theme	Hepatitis	167:175	arg1	virus					179:183	Hepatitis B virus	167:183	Hepatitis B virus (HBV)	167:189	Hepatitis B virus (HBV) expresses three co-terminal envelope proteins: large (L), middle (M), and small (S), with the S protein driving the secretion of both virions and subviral particles.
29604477	1	34	theme	Hepatitis	167:175	arg1	HBV					186:188	HBV	186:188	HBV	186:188	Hepatitis B virus (HBV) expresses three co-terminal envelope proteins: large (L), middle (M), and small (S), with the S protein driving the secretion of both virions and subviral particles.
29604477	0	35	theme	escape	17:22	arg1	mutations					24:32	immune escape mutations	10:32	immune escape mutations	10:32	Impact of immune escape mutations and N-linked glycosylation on the secretion of hepatitis B virus virions and subviral particles: Role of the small envelope protein.
29604477	3	36	theme	mutants	641:647	arg1	secretion					559:567	virion secretion	552:567	virion secretion of N146Q mutant (loss of original glycosylation site) and immune escape mutants such as G145R	552:661	An M133T mutation creating a novel glycosylation site at N131could rescue virion secretion of N146Q mutant (loss of original glycosylation site) and immune escape mutants such as G145R.
29604477	0	37	theme	subviral	111:118	arg1	particles					120:128	subviral particles	111:128	subviral particles	111:128	Impact of immune escape mutations and N-linked glycosylation on the secretion of hepatitis B virus virions and subviral particles: Role of the small envelope protein.
29604477	5	38	theme	N146Q	827:831	arg1	mutations					833:841	Both G145R and N146Q mutations	812:841	Both G145R and N146Q mutations	812:841	Both G145R and N146Q mutations impaired virion secretion through the S protein.
29604477	4	39	theme	novel	695:699	arg1	sites					724:728	other novel N-linked glycosylation sites	689:728	other novel N-linked glycosylation sites	689:728	Here we demonstrate that other novel N-linked glycosylation sites could rescue virion secretion of the G145R and N146Q mutants to variable extents.
29604477	1	40	theme	B	177:177	arg1	virus					179:183	Hepatitis B virus	167:183	Hepatitis B virus (HBV)	167:189	Hepatitis B virus (HBV) expresses three co-terminal envelope proteins: large (L), middle (M), and small (S), with the S protein driving the secretion of both virions and subviral particles.
29604477	1	40	theme	B	177:177	arg1	HBV					186:188	HBV	186:188	HBV	186:188	Hepatitis B virus (HBV) expresses three co-terminal envelope proteins: large (L), middle (M), and small (S), with the S protein driving the secretion of both virions and subviral particles.
29604477	0	41	theme	immune	10:15	arg1	mutations					24:32	immune escape mutations	10:32	immune escape mutations	10:32	Impact of immune escape mutations and N-linked glycosylation on the secretion of hepatitis B virus virions and subviral particles: Role of the small envelope protein.
29604477	3	42	gly	glycosylation	603:615	arg2	site					617:620	original glycosylation site	594:620	original glycosylation site	594:620	An M133T mutation creating a novel glycosylation site at N131could rescue virion secretion of N146Q mutant (loss of original glycosylation site) and immune escape mutants such as G145R.
29604477	4	43	theme	variable	794:801	arg1	extents					803:809	variable extents	794:809	variable extents	794:809	Here we demonstrate that other novel N-linked glycosylation sites could rescue virion secretion of the G145R and N146Q mutants to variable extents.
29604477	2	44	gly	glycosylation	392:404	arg1	N146					409:412	N146	409:412	N146	409:412	Virion secretion requires N-linked glycosylation at N146 in the S domain but can be impaired by immune escape mutations.
29604477	2	44	gly	glycosylation	392:404	arg1	domain					423:428	the S domain	417:428	the S domain	417:428	Virion secretion requires N-linked glycosylation at N146 in the S domain but can be impaired by immune escape mutations.
29604477	5	45	theme	virion	852:857	arg1	secretion					859:867	virion secretion	852:867	virion secretion	852:867	Both G145R and N146Q mutations impaired virion secretion through the S protein.
29604477	4	46	dep	G145R	767:771	arg1	mutants					783:789	mutants	783:789	mutants	783:789	Here we demonstrate that other novel N-linked glycosylation sites could rescue virion secretion of the G145R and N146Q mutants to variable extents.
29604477	4	46	dep	G145R	767:771	arg1	the					763:765	the	763:765	the	763:765	Here we demonstrate that other novel N-linked glycosylation sites could rescue virion secretion of the G145R and N146Q mutants to variable extents.
29604477	3	47	theme	immune	627:632	arg1	mutants					641:647	immune escape mutants	627:647	immune escape mutants such as G145R	627:661	An M133T mutation creating a novel glycosylation site at N131could rescue virion secretion of N146Q mutant (loss of original glycosylation site) and immune escape mutants such as G145R.
29604477	3	47	theme	immune	627:632	arg1	G145R					657:661	G145R	657:661	G145R	657:661	An M133T mutation creating a novel glycosylation site at N131could rescue virion secretion of N146Q mutant (loss of original glycosylation site) and immune escape mutants such as G145R.
29604477	1	48	theme	S	285:285	arg1	protein					287:293	the S protein	281:293	the S protein driving the secretion of both virions and subviral particles	281:354	Hepatitis B virus (HBV) expresses three co-terminal envelope proteins: large (L), middle (M), and small (S), with the S protein driving the secretion of both virions and subviral particles.
29604477	3	49	theme	M133T	481:485	arg1	mutation					487:494	An M133T mutation	478:494	An M133T mutation creating a novel glycosylation site at N131could	478:543	An M133T mutation creating a novel glycosylation site at N131could rescue virion secretion of N146Q mutant (loss of original glycosylation site) and immune escape mutants such as G145R.
29604477	6	50	theme	S	949:949	arg1	protein					951:957	the S protein	945:957	the S protein	945:957	The M133T mutation restored virion secretion through the S protein, and could work in trans.
29604477	0	51	theme	mutations	24:32	arg1	Impact					0:5	Impact	0:5	Impact of immune escape mutations and N-linked glycosylation on the secretion of hepatitis B virus virions and subviral particles: Role of the small envelope protein.	0:165	Impact of immune escape mutations and N-linked glycosylation on the secretion of hepatitis B virus virions and subviral particles: Role of the small envelope protein.
29604477	7	52	theme	particles	1092:1100	arg1	secretion					1070:1078	the secretion	1066:1078	the secretion of subviral particles	1066:1100	Impaired virion secretion was not necessarily associated with a similar block in the secretion of subviral particles.
29604477	0	53	theme	particles	120:128	arg1	secretion					68:76	the secretion	64:76	the secretion of hepatitis B virus virions and subviral particles	64:128	Impact of immune escape mutations and N-linked glycosylation on the secretion of hepatitis B virus virions and subviral particles: Role of the small envelope protein.
29604477	4	54	theme	other	689:693	arg1	sites					724:728	other novel N-linked glycosylation sites	689:728	other novel N-linked glycosylation sites	689:728	Here we demonstrate that other novel N-linked glycosylation sites could rescue virion secretion of the G145R and N146Q mutants to variable extents.
29604477	6	55	theme	virion	920:925	arg1	secretion					927:935	virion secretion	920:935	virion secretion	920:935	The M133T mutation restored virion secretion through the S protein, and could work in trans.
29604477	0	56	theme	glycosylation	47:59	arg1	Impact					0:5	Impact	0:5	Impact of immune escape mutations and N-linked glycosylation on the secretion of hepatitis B virus virions and subviral particles: Role of the small envelope protein.	0:165	Impact of immune escape mutations and N-linked glycosylation on the secretion of hepatitis B virus virions and subviral particles: Role of the small envelope protein.
29604477	4	57	theme	N146Q	777:781	arg1	secretion					750:758	virion secretion	743:758	virion secretion of the G145R and N146Q mutants to variable extents	743:809	Here we demonstrate that other novel N-linked glycosylation sites could rescue virion secretion of the G145R and N146Q mutants to variable extents.
29604477	2	58	theme	S	421:421	arg1	domain					423:428	the S domain	417:428	the S domain	417:428	Virion secretion requires N-linked glycosylation at N146 in the S domain but can be impaired by immune escape mutations.
29604477	2	59	link	N-linked	383:390	arg1	glycosylation					392:404	N-linked glycosylation	383:404	N-linked glycosylation	383:404	Virion secretion requires N-linked glycosylation at N146 in the S domain but can be impaired by immune escape mutations.
29604477	0	60	theme	N-linked	38:45	arg1	glycosylation					47:59	N-linked glycosylation	38:59	N-linked glycosylation	38:59	Impact of immune escape mutations and N-linked glycosylation on the secretion of hepatitis B virus virions and subviral particles: Role of the small envelope protein.
29604477	2	61	theme	escape	460:465	arg1	mutations					467:475	immune escape mutations	453:475	immune escape mutations	453:475	Virion secretion requires N-linked glycosylation at N146 in the S domain but can be impaired by immune escape mutations.
29604477	4	62	theme	N-linked	701:708	arg1	sites					724:728	other novel N-linked glycosylation sites	689:728	other novel N-linked glycosylation sites	689:728	Here we demonstrate that other novel N-linked glycosylation sites could rescue virion secretion of the G145R and N146Q mutants to variable extents.
29604477	0	63	theme	small	143:147	arg1	protein					158:164	the small envelope protein	139:164	the small envelope protein	139:164	Impact of immune escape mutations and N-linked glycosylation on the secretion of hepatitis B virus virions and subviral particles: Role of the small envelope protein.
29604477	3	64	theme	N146Q	572:576	arg1	mutant					578:583	N146Q mutant	572:583	N146Q mutant (loss of original glycosylation site)	572:621	An M133T mutation creating a novel glycosylation site at N131could rescue virion secretion of N146Q mutant (loss of original glycosylation site) and immune escape mutants such as G145R.
29604477	3	65	theme	novel	507:511	arg1	site					527:530	a novel glycosylation site	505:530	a novel glycosylation site at N131could	505:543	An M133T mutation creating a novel glycosylation site at N131could rescue virion secretion of N146Q mutant (loss of original glycosylation site) and immune escape mutants such as G145R.
29604477	3	66	theme	virion	552:557	arg1	secretion					559:567	virion secretion	552:567	virion secretion of N146Q mutant (loss of original glycosylation site) and immune escape mutants such as G145R	552:661	An M133T mutation creating a novel glycosylation site at N131could rescue virion secretion of N146Q mutant (loss of original glycosylation site) and immune escape mutants such as G145R.
29604477	3	67	theme	glycosylation	513:525	arg1	site					527:530	a novel glycosylation site	505:530	a novel glycosylation site at N131could	505:543	An M133T mutation creating a novel glycosylation site at N131could rescue virion secretion of N146Q mutant (loss of original glycosylation site) and immune escape mutants such as G145R.
29604477	1	68	dep	proteins	228:235	arg1	M					257:257	M	257:257	M	257:257	Hepatitis B virus (HBV) expresses three co-terminal envelope proteins: large (L), middle (M), and small (S), with the S protein driving the secretion of both virions and subviral particles.
29604477	1	68	dep	proteins	228:235	arg1	S					272:272	S	272:272	S	272:272	Hepatitis B virus (HBV) expresses three co-terminal envelope proteins: large (L), middle (M), and small (S), with the S protein driving the secretion of both virions and subviral particles.
29604477	1	68	dep	proteins	228:235	arg1	L					245:245	L	245:245	L	245:245	Hepatitis B virus (HBV) expresses three co-terminal envelope proteins: large (L), middle (M), and small (S), with the S protein driving the secretion of both virions and subviral particles.
29604477	1	68	dep	proteins	228:235	arg1	small					265:269	small	265:269	small	265:269	Hepatitis B virus (HBV) expresses three co-terminal envelope proteins: large (L), middle (M), and small (S), with the S protein driving the secretion of both virions and subviral particles.
29604477	1	68	dep	proteins	228:235	arg1	middle					249:254	middle	249:254	middle	249:254	Hepatitis B virus (HBV) expresses three co-terminal envelope proteins: large (L), middle (M), and small (S), with the S protein driving the secretion of both virions and subviral particles.
29604477	1	68	dep	proteins	228:235	arg1	large					238:242	large	238:242	large	238:242	Hepatitis B virus (HBV) expresses three co-terminal envelope proteins: large (L), middle (M), and small (S), with the S protein driving the secretion of both virions and subviral particles.
29604477	6	69	theme	M133T	896:900	arg1	mutation					902:909	The M133T mutation	892:909	The M133T mutation	892:909	The M133T mutation restored virion secretion through the S protein, and could work in trans.
29604477	2	70	theme	Virion	357:362	arg1	secretion					364:372	Virion secretion	357:372	Virion secretion	357:372	Virion secretion requires N-linked glycosylation at N146 in the S domain but can be impaired by immune escape mutations.
29604477	0	71	dep	Impact	0:5	arg1	Role					131:134	Role	131:134	Impact of immune escape mutations and N-linked glycosylation on the secretion of hepatitis B virus virions and subviral particles: Role of the small envelope protein.	0:165	Impact of immune escape mutations and N-linked glycosylation on the secretion of hepatitis B virus virions and subviral particles: Role of the small envelope protein.
29604477	1	72	theme	co-terminal	207:217	arg1	proteins					228:235	three co-terminal envelope proteins	201:235	three co-terminal envelope proteins: large (L), middle (M), and small (S)	201:273	Hepatitis B virus (HBV) expresses three co-terminal envelope proteins: large (L), middle (M), and small (S), with the S protein driving the secretion of both virions and subviral particles.
29303997	7	0	theme	tetherin	1147:1154	arg1	expression					1156:1165	cell-surface tetherin expression	1134:1165	cell-surface tetherin expression	1134:1165	A role for glycosylation in cell-surface tetherin expression is supported by tunicamycin treatment, which inhibits the first step of N-linked glycosylation and impairs both cell-surface expression and antiviral activity.
29303997	7	1	from	role	1108:1111	arg1	expression					1156:1165	cell-surface tetherin expression	1134:1165	cell-surface tetherin expression	1134:1165	A role for glycosylation in cell-surface tetherin expression is supported by tunicamycin treatment, which inhibits the first step of N-linked glycosylation and impairs both cell-surface expression and antiviral activity.
29303997	2	2	from	role	292:295	arg1	activity					339:346	antiviral activity	329:346	antiviral activity	329:346	While the role of specific tetherin domains in antiviral activity is clearly established, the role of glycosylation in tetherin function is not clear.
29303997	6	3	theme	cell-surface	1081:1092	arg1	expression					1094:1103	cell-surface expression	1081:1103	cell-surface expression	1081:1103	The inability of the N65,92A mutant to inhibit HIV-1 release is associated with a lack of cell-surface expression.
29303997	6	4	theme	mutant	1020:1025	arg1	inability					995:1003	The inability	991:1003	The inability of the N65,92A mutant to inhibit HIV-1 release	991:1050	The inability of the N65,92A mutant to inhibit HIV-1 release is associated with a lack of cell-surface expression.
29303997	0	5	theme	Release	97:103	arg1	Restriction					76:86	Restriction	76:86	Restriction of HIV-1 Release	76:103	High-Mannose But Not Complex-Type Glycosylation of Tetherin Is Required for Restriction of HIV-1 Release.
29303997	3	6	theme	chemical	629:636	arg1	inhibitors					638:647	chemical inhibitors	629:647	chemical inhibitors that prevent glycosylation at specific stages of oligosaccharide	629:712	In this study, we carried out a detailed investigation of this question by using tetherin variants in which one or both sites of N-linked glycosylation were mutated (N65A, N92A, and N65,92A), and chemical inhibitors that prevent glycosylation at specific stages of oligosaccharide were added or modified.
29303997	4	7	theme	Vpu-defective	833:845	arg1	type					876:879	Vpu-defective human immunodeficiency virus type 1	833:881	Vpu-defective human immunodeficiency virus type 1 (HIV-1)	833:889	The single N-linked glycosylation mutants, N65A and N92A, efficiently inhibited the release of Vpu-defective human immunodeficiency virus type 1 (HIV-1).
29303997	4	7	theme	Vpu-defective	833:845	arg1	HIV-1					884:888	HIV-1	884:888	HIV-1	884:888	The single N-linked glycosylation mutants, N65A and N92A, efficiently inhibited the release of Vpu-defective human immunodeficiency virus type 1 (HIV-1).
29303997	3	8	theme	tetherin	514:521	arg1	variants					523:530	tetherin variants	514:530	tetherin variants in which one or both sites of N-linked glycosylation were mutated (N65A, N92A, and N65,92A), and chemical inhibitors that prevent glycosylation at specific stages of oligosaccharide were added or modified	514:735	In this study, we carried out a detailed investigation of this question by using tetherin variants in which one or both sites of N-linked glycosylation were mutated (N65A, N92A, and N65,92A), and chemical inhibitors that prevent glycosylation at specific stages of oligosaccharide were added or modified.
29303997	8	9	theme	tetherin	1532:1539	arg1	effect					1462:1467	no effect	1459:1467	no effect on either the cell-surface expression or antiviral activity of tetherin	1459:1539	Inhibition of complex-type glycosylation with kifunensine, an inhibitor of the oligosaccharide processing enzyme mannosidase 1, had no effect on either the cell-surface expression or antiviral activity of tetherin.
29303997	0	10	theme	HIV-1	91:95	arg1	Release					97:103	HIV-1 Release	91:103	HIV-1 Release	91:103	High-Mannose But Not Complex-Type Glycosylation of Tetherin Is Required for Restriction of HIV-1 Release.
29303997	8	11	theme	oligosaccharide	1406:1420	arg1	mannosidase					1440:1450	the oligosaccharide processing enzyme mannosidase 1	1402:1452	the oligosaccharide processing enzyme mannosidase 1	1402:1452	Inhibition of complex-type glycosylation with kifunensine, an inhibitor of the oligosaccharide processing enzyme mannosidase 1, had no effect on either the cell-surface expression or antiviral activity of tetherin.
29303997	5	12	theme	non-glycosylated	909:924	arg1	N65,92A					941:947	N65,92A	941:947	N65,92A	941:947	In contrast, the non-glycosylated double mutant, N65,92A, lost its ability to block HIV-1 release.
29303997	5	12	theme	non-glycosylated	909:924	arg1	mutant					933:938	the non-glycosylated double mutant	905:938	the non-glycosylated double mutant	905:938	In contrast, the non-glycosylated double mutant, N65,92A, lost its ability to block HIV-1 release.
29303997	1	13	theme	infected	266:273	arg1	cells					275:279	infected cells	266:279	infected cells	266:279	Tetherin is an interferon-inducible antiviral protein that inhibits the release of a broad spectrum of enveloped viruses by retaining virions at the surface of infected cells.
29303997	3	14	contain	carried	451:457	arg3	study					441:445	this study	436:445	this study	436:445	In this study, we carried out a detailed investigation of this question by using tetherin variants in which one or both sites of N-linked glycosylation were mutated (N65A, N92A, and N65,92A), and chemical inhibitors that prevent glycosylation at specific stages of oligosaccharide were added or modified.
29303997	3	14	contain	carried	451:457	arg2	investigation					474:486	a detailed investigation	463:486	a detailed investigation of this question	463:503	In this study, we carried out a detailed investigation of this question by using tetherin variants in which one or both sites of N-linked glycosylation were mutated (N65A, N92A, and N65,92A), and chemical inhibitors that prevent glycosylation at specific stages of oligosaccharide were added or modified.
29303997	3	14	contain	carried	451:457	arg1	we					448:449	we	448:449	we	448:449	In this study, we carried out a detailed investigation of this question by using tetherin variants in which one or both sites of N-linked glycosylation were mutated (N65A, N92A, and N65,92A), and chemical inhibitors that prevent glycosylation at specific stages of oligosaccharide were added or modified.
29303997	9	15	theme	tetherin	1725:1732	arg1	expression					1734:1743	cell-surface tetherin expression	1712:1743	cell-surface tetherin expression	1712:1743	These results demonstrate that high-mannose modification of a single asparagine residue is necessary and sufficient, while complex-type glycosylation is dispensable, for cell-surface tetherin expression and antiviral activity.
29303997	7	16	theme	glycosylation	1248:1260	arg1	step					1231:1234	the first step	1221:1234	the first step of N-linked glycosylation	1221:1260	A role for glycosylation in cell-surface tetherin expression is supported by tunicamycin treatment, which inhibits the first step of N-linked glycosylation and impairs both cell-surface expression and antiviral activity.
29303997	5	17	theme	double	926:931	arg1	N65,92A					941:947	N65,92A	941:947	N65,92A	941:947	In contrast, the non-glycosylated double mutant, N65,92A, lost its ability to block HIV-1 release.
29303997	5	17	theme	double	926:931	arg1	mutant					933:938	the non-glycosylated double mutant	905:938	the non-glycosylated double mutant	905:938	In contrast, the non-glycosylated double mutant, N65,92A, lost its ability to block HIV-1 release.
29303997	2	18	theme	glycosylation	384:396	arg1	role					376:379	the role	372:379	the role of glycosylation in tetherin function	372:417	While the role of specific tetherin domains in antiviral activity is clearly established, the role of glycosylation in tetherin function is not clear.
29303997	2	18	theme	glycosylation	384:396	arg1	clear					426:430	clear	426:430	clear	426:430	While the role of specific tetherin domains in antiviral activity is clearly established, the role of glycosylation in tetherin function is not clear.
29303997	3	19	link	N-linked	562:569	arg1	glycosylation					571:583	N-linked glycosylation	562:583	N-linked glycosylation	562:583	In this study, we carried out a detailed investigation of this question by using tetherin variants in which one or both sites of N-linked glycosylation were mutated (N65A, N92A, and N65,92A), and chemical inhibitors that prevent glycosylation at specific stages of oligosaccharide were added or modified.
29303997	8	20	theme	antiviral	1510:1518	arg1	activity					1520:1527	antiviral activity	1510:1527	antiviral activity	1510:1527	Inhibition of complex-type glycosylation with kifunensine, an inhibitor of the oligosaccharide processing enzyme mannosidase 1, had no effect on either the cell-surface expression or antiviral activity of tetherin.
29303997	2	21	gly	glycosylation	384:396	arg1	tetherin					401:408	tetherin function	401:417	tetherin function	401:417	While the role of specific tetherin domains in antiviral activity is clearly established, the role of glycosylation in tetherin function is not clear.
29303997	7	22	link	N-linked	1239:1246	arg1	glycosylation					1248:1260	N-linked glycosylation	1239:1260	N-linked glycosylation	1239:1260	A role for glycosylation in cell-surface tetherin expression is supported by tunicamycin treatment, which inhibits the first step of N-linked glycosylation and impairs both cell-surface expression and antiviral activity.
29303997	6	23	theme	expression	1094:1103	arg1	lack					1073:1076	a lack	1071:1076	a lack of cell-surface expression	1071:1103	The inability of the N65,92A mutant to inhibit HIV-1 release is associated with a lack of cell-surface expression.
29303997	7	24	theme	cell-surface	1279:1290	arg1	expression					1292:1301	cell-surface expression	1279:1301	cell-surface expression	1279:1301	A role for glycosylation in cell-surface tetherin expression is supported by tunicamycin treatment, which inhibits the first step of N-linked glycosylation and impairs both cell-surface expression and antiviral activity.
29303997	1	25	theme	antiviral	142:150	arg1	protein					152:158	an interferon-inducible antiviral protein	118:158	an interferon-inducible antiviral protein that inhibits the release of a broad spectrum of enveloped viruses by retaining virions at the surface of infected cells	118:279	Tetherin is an interferon-inducible antiviral protein that inhibits the release of a broad spectrum of enveloped viruses by retaining virions at the surface of infected cells.
29303997	1	25	theme	antiviral	142:150	arg1	Tetherin					106:113	Tetherin	106:113	Tetherin	106:113	Tetherin is an interferon-inducible antiviral protein that inhibits the release of a broad spectrum of enveloped viruses by retaining virions at the surface of infected cells.
29303997	3	26	theme	oligosaccharide	698:712	arg1	stages					688:693	specific stages	679:693	specific stages of oligosaccharide	679:712	In this study, we carried out a detailed investigation of this question by using tetherin variants in which one or both sites of N-linked glycosylation were mutated (N65A, N92A, and N65,92A), and chemical inhibitors that prevent glycosylation at specific stages of oligosaccharide were added or modified.
29303997	2	27	theme	tetherin	309:316	arg1	domains					318:324	specific tetherin domains	300:324	specific tetherin domains	300:324	While the role of specific tetherin domains in antiviral activity is clearly established, the role of glycosylation in tetherin function is not clear.
29303997	3	28	theme	specific	679:686	arg1	stages					688:693	specific stages	679:693	specific stages of oligosaccharide	679:712	In this study, we carried out a detailed investigation of this question by using tetherin variants in which one or both sites of N-linked glycosylation were mutated (N65A, N92A, and N65,92A), and chemical inhibitors that prevent glycosylation at specific stages of oligosaccharide were added or modified.
29303997	0	29	theme	High-Mannose	0:11	arg1	Glycosylation					34:46	High-Mannose But Not Complex-Type Glycosylation	0:46	High-Mannose But Not Complex-Type Glycosylation of Tetherin	0:58	High-Mannose But Not Complex-Type Glycosylation of Tetherin Is Required for Restriction of HIV-1 Release.
29303997	1	30	theme	cells	275:279	arg1	surface					255:261	the surface	251:261	the surface of infected cells	251:279	Tetherin is an interferon-inducible antiviral protein that inhibits the release of a broad spectrum of enveloped viruses by retaining virions at the surface of infected cells.
29303997	9	31	theme	asparagine	1611:1620	arg1	residue					1622:1628	a single asparagine residue	1602:1628	a single asparagine residue	1602:1628	These results demonstrate that high-mannose modification of a single asparagine residue is necessary and sufficient, while complex-type glycosylation is dispensable, for cell-surface tetherin expression and antiviral activity.
29303997	2	32	theme	specific	300:307	arg1	domains					318:324	specific tetherin domains	300:324	specific tetherin domains	300:324	While the role of specific tetherin domains in antiviral activity is clearly established, the role of glycosylation in tetherin function is not clear.
29303997	1	33	theme	broad	191:195	arg1	spectrum					197:204	a broad spectrum	189:204	a broad spectrum of enveloped viruses	189:225	Tetherin is an interferon-inducible antiviral protein that inhibits the release of a broad spectrum of enveloped viruses by retaining virions at the surface of infected cells.
29303997	0	34	theme	Complex-Type	21:32	arg1	Glycosylation					34:46	High-Mannose But Not Complex-Type Glycosylation	0:46	High-Mannose But Not Complex-Type Glycosylation of Tetherin	0:58	High-Mannose But Not Complex-Type Glycosylation of Tetherin Is Required for Restriction of HIV-1 Release.
29303997	4	35	theme	glycosylation	758:770	arg1	N92A					790:793	N92A	790:793	N92A	790:793	The single N-linked glycosylation mutants, N65A and N92A, efficiently inhibited the release of Vpu-defective human immunodeficiency virus type 1 (HIV-1).
29303997	4	35	theme	glycosylation	758:770	arg1	N65A					781:784	N65A	781:784	N65A	781:784	The single N-linked glycosylation mutants, N65A and N92A, efficiently inhibited the release of Vpu-defective human immunodeficiency virus type 1 (HIV-1).
29303997	4	35	theme	glycosylation	758:770	arg1	mutants					772:778	The single N-linked glycosylation mutants	738:778	The single N-linked glycosylation mutants	738:778	The single N-linked glycosylation mutants, N65A and N92A, efficiently inhibited the release of Vpu-defective human immunodeficiency virus type 1 (HIV-1).
29303997	9	36	theme	complex-type	1665:1676	arg1	glycosylation					1678:1690	complex-type glycosylation	1665:1690	complex-type glycosylation	1665:1690	These results demonstrate that high-mannose modification of a single asparagine residue is necessary and sufficient, while complex-type glycosylation is dispensable, for cell-surface tetherin expression and antiviral activity.
29303997	8	37	theme	complex-type	1341:1352	arg1	glycosylation					1354:1366	complex-type glycosylation	1341:1366	complex-type glycosylation	1341:1366	Inhibition of complex-type glycosylation with kifunensine, an inhibitor of the oligosaccharide processing enzyme mannosidase 1, had no effect on either the cell-surface expression or antiviral activity of tetherin.
29303997	1	38	theme	spectrum	197:204	arg1	release					178:184	the release	174:184	the release of a broad spectrum of enveloped viruses	174:225	Tetherin is an interferon-inducible antiviral protein that inhibits the release of a broad spectrum of enveloped viruses by retaining virions at the surface of infected cells.
29303997	8	39	from	effect	1462:1467	arg1	expression					1496:1505	the cell-surface expression	1479:1505	the cell-surface expression	1479:1505	Inhibition of complex-type glycosylation with kifunensine, an inhibitor of the oligosaccharide processing enzyme mannosidase 1, had no effect on either the cell-surface expression or antiviral activity of tetherin.
29303997	8	39	from	effect	1462:1467	arg1	activity					1520:1527	antiviral activity	1510:1527	antiviral activity	1510:1527	Inhibition of complex-type glycosylation with kifunensine, an inhibitor of the oligosaccharide processing enzyme mannosidase 1, had no effect on either the cell-surface expression or antiviral activity of tetherin.
29303997	3	40	gly	glycosylation	662:674	arg1	stages					688:693	specific stages	679:693	specific stages of oligosaccharide	679:712	In this study, we carried out a detailed investigation of this question by using tetherin variants in which one or both sites of N-linked glycosylation were mutated (N65A, N92A, and N65,92A), and chemical inhibitors that prevent glycosylation at specific stages of oligosaccharide were added or modified.
29303997	8	41	theme	processing	1422:1431	arg1	mannosidase					1440:1450	the oligosaccharide processing enzyme mannosidase 1	1402:1452	the oligosaccharide processing enzyme mannosidase 1	1402:1452	Inhibition of complex-type glycosylation with kifunensine, an inhibitor of the oligosaccharide processing enzyme mannosidase 1, had no effect on either the cell-surface expression or antiviral activity of tetherin.
29303997	7	42	theme	tunicamycin	1183:1193	arg1	treatment					1195:1203	tunicamycin treatment	1183:1203	tunicamycin treatment	1183:1203	A role for glycosylation in cell-surface tetherin expression is supported by tunicamycin treatment, which inhibits the first step of N-linked glycosylation and impairs both cell-surface expression and antiviral activity.
29303997	4	43	link	N-linked	749:756	arg1	N92A					790:793	N92A	790:793	N92A	790:793	The single N-linked glycosylation mutants, N65A and N92A, efficiently inhibited the release of Vpu-defective human immunodeficiency virus type 1 (HIV-1).
29303997	4	43	link	N-linked	749:756	arg1	N65A					781:784	N65A	781:784	N65A	781:784	The single N-linked glycosylation mutants, N65A and N92A, efficiently inhibited the release of Vpu-defective human immunodeficiency virus type 1 (HIV-1).
29303997	4	43	link	N-linked	749:756	arg1	mutants					772:778	The single N-linked glycosylation mutants	738:778	The single N-linked glycosylation mutants	738:778	The single N-linked glycosylation mutants, N65A and N92A, efficiently inhibited the release of Vpu-defective human immunodeficiency virus type 1 (HIV-1).
29303997	3	44	theme	detailed	465:472	arg1	investigation					474:486	a detailed investigation	463:486	a detailed investigation of this question	463:503	In this study, we carried out a detailed investigation of this question by using tetherin variants in which one or both sites of N-linked glycosylation were mutated (N65A, N92A, and N65,92A), and chemical inhibitors that prevent glycosylation at specific stages of oligosaccharide were added or modified.
29303997	1	45	theme	enveloped	209:217	arg1	viruses					219:225	enveloped viruses	209:225	enveloped viruses	209:225	Tetherin is an interferon-inducible antiviral protein that inhibits the release of a broad spectrum of enveloped viruses by retaining virions at the surface of infected cells.
29303997	9	46	theme	high-mannose	1573:1584	arg1	modification					1586:1597	high-mannose modification	1573:1597	high-mannose modification of a single asparagine residue	1573:1628	These results demonstrate that high-mannose modification of a single asparagine residue is necessary and sufficient, while complex-type glycosylation is dispensable, for cell-surface tetherin expression and antiviral activity.
29303997	2	47	theme	tetherin	401:408	arg1	function					410:417	tetherin function	401:417	tetherin function	401:417	While the role of specific tetherin domains in antiviral activity is clearly established, the role of glycosylation in tetherin function is not clear.
29303997	6	48	theme	N65,92A	1012:1018	arg1	mutant					1020:1025	the N65,92A mutant	1008:1025	the N65,92A mutant	1008:1025	The inability of the N65,92A mutant to inhibit HIV-1 release is associated with a lack of cell-surface expression.
29303997	9	49	theme	cell-surface	1712:1723	arg1	expression					1734:1743	cell-surface tetherin expression	1712:1743	cell-surface tetherin expression	1712:1743	These results demonstrate that high-mannose modification of a single asparagine residue is necessary and sufficient, while complex-type glycosylation is dispensable, for cell-surface tetherin expression and antiviral activity.
29303997	8	50	theme	mannosidase	1440:1450	arg1	kifunensine					1373:1383	kifunensine	1373:1383	kifunensine	1373:1383	Inhibition of complex-type glycosylation with kifunensine, an inhibitor of the oligosaccharide processing enzyme mannosidase 1, had no effect on either the cell-surface expression or antiviral activity of tetherin.
29303997	8	50	theme	mannosidase	1440:1450	arg1	inhibitor					1389:1397	an inhibitor	1386:1397	an inhibitor of the oligosaccharide processing enzyme mannosidase 1	1386:1452	Inhibition of complex-type glycosylation with kifunensine, an inhibitor of the oligosaccharide processing enzyme mannosidase 1, had no effect on either the cell-surface expression or antiviral activity of tetherin.
29303997	3	51	theme	N-linked	562:569	arg1	glycosylation					571:583	N-linked glycosylation	562:583	N-linked glycosylation	562:583	In this study, we carried out a detailed investigation of this question by using tetherin variants in which one or both sites of N-linked glycosylation were mutated (N65A, N92A, and N65,92A), and chemical inhibitors that prevent glycosylation at specific stages of oligosaccharide were added or modified.
29303997	2	52	from	role	376:379	arg1	function					410:417	tetherin function	401:417	tetherin function	401:417	While the role of specific tetherin domains in antiviral activity is clearly established, the role of glycosylation in tetherin function is not clear.
29303997	1	53	theme	viruses	219:225	arg1	spectrum					197:204	a broad spectrum	189:204	a broad spectrum of enveloped viruses	189:225	Tetherin is an interferon-inducible antiviral protein that inhibits the release of a broad spectrum of enveloped viruses by retaining virions at the surface of infected cells.
29303997	9	54	theme	antiviral	1749:1757	arg1	activity					1759:1766	antiviral activity	1749:1766	antiviral activity	1749:1766	These results demonstrate that high-mannose modification of a single asparagine residue is necessary and sufficient, while complex-type glycosylation is dispensable, for cell-surface tetherin expression and antiviral activity.
29303997	4	55	theme	immunodeficiency	853:868	arg1	type					876:879	Vpu-defective human immunodeficiency virus type 1	833:881	Vpu-defective human immunodeficiency virus type 1 (HIV-1)	833:889	The single N-linked glycosylation mutants, N65A and N92A, efficiently inhibited the release of Vpu-defective human immunodeficiency virus type 1 (HIV-1).
29303997	4	55	theme	immunodeficiency	853:868	arg1	HIV-1					884:888	HIV-1	884:888	HIV-1	884:888	The single N-linked glycosylation mutants, N65A and N92A, efficiently inhibited the release of Vpu-defective human immunodeficiency virus type 1 (HIV-1).
29303997	8	56	theme	enzyme	1433:1438	arg1	mannosidase					1440:1450	the oligosaccharide processing enzyme mannosidase 1	1402:1452	the oligosaccharide processing enzyme mannosidase 1	1402:1452	Inhibition of complex-type glycosylation with kifunensine, an inhibitor of the oligosaccharide processing enzyme mannosidase 1, had no effect on either the cell-surface expression or antiviral activity of tetherin.
29303997	3	57	dep	mutated	590:596	arg1	N92A					605:608	N92A	605:608	N92A	605:608	In this study, we carried out a detailed investigation of this question by using tetherin variants in which one or both sites of N-linked glycosylation were mutated (N65A, N92A, and N65,92A), and chemical inhibitors that prevent glycosylation at specific stages of oligosaccharide were added or modified.
29303997	3	57	dep	mutated	590:596	arg1	N65,92A					615:621	N65,92A	615:621	N65,92A	615:621	In this study, we carried out a detailed investigation of this question by using tetherin variants in which one or both sites of N-linked glycosylation were mutated (N65A, N92A, and N65,92A), and chemical inhibitors that prevent glycosylation at specific stages of oligosaccharide were added or modified.
29303997	3	57	dep	mutated	590:596	arg1	N65A					599:602	N65A	599:602	N65A	599:602	In this study, we carried out a detailed investigation of this question by using tetherin variants in which one or both sites of N-linked glycosylation were mutated (N65A, N92A, and N65,92A), and chemical inhibitors that prevent glycosylation at specific stages of oligosaccharide were added or modified.
29303997	0	58	theme	Tetherin	51:58	arg1	Glycosylation					34:46	High-Mannose But Not Complex-Type Glycosylation	0:46	High-Mannose But Not Complex-Type Glycosylation of Tetherin	0:58	High-Mannose But Not Complex-Type Glycosylation of Tetherin Is Required for Restriction of HIV-1 Release.
29303997	8	59	theme	cell-surface	1483:1494	arg1	expression					1496:1505	the cell-surface expression	1479:1505	the cell-surface expression	1479:1505	Inhibition of complex-type glycosylation with kifunensine, an inhibitor of the oligosaccharide processing enzyme mannosidase 1, had no effect on either the cell-surface expression or antiviral activity of tetherin.
29303997	4	60	theme	human	847:851	arg1	type					876:879	Vpu-defective human immunodeficiency virus type 1	833:881	Vpu-defective human immunodeficiency virus type 1 (HIV-1)	833:889	The single N-linked glycosylation mutants, N65A and N92A, efficiently inhibited the release of Vpu-defective human immunodeficiency virus type 1 (HIV-1).
29303997	4	60	theme	human	847:851	arg1	HIV-1					884:888	HIV-1	884:888	HIV-1	884:888	The single N-linked glycosylation mutants, N65A and N92A, efficiently inhibited the release of Vpu-defective human immunodeficiency virus type 1 (HIV-1).
29303997	5	61	theme	HIV-1	976:980	arg1	release					982:988	HIV-1 release	976:988	HIV-1 release	976:988	In contrast, the non-glycosylated double mutant, N65,92A, lost its ability to block HIV-1 release.
29303997	9	62	theme	residue	1622:1628	arg1	modification					1586:1597	high-mannose modification	1573:1597	high-mannose modification of a single asparagine residue	1573:1628	These results demonstrate that high-mannose modification of a single asparagine residue is necessary and sufficient, while complex-type glycosylation is dispensable, for cell-surface tetherin expression and antiviral activity.
29303997	2	63	theme	antiviral	329:337	arg1	activity					339:346	antiviral activity	329:346	antiviral activity	329:346	While the role of specific tetherin domains in antiviral activity is clearly established, the role of glycosylation in tetherin function is not clear.
29303997	8	64	contain	had	1455:1457	arg1	Inhibition					1327:1336	Inhibition	1327:1336	Inhibition of complex-type glycosylation with kifunensine, an inhibitor of the oligosaccharide processing enzyme mannosidase 1,	1327:1453	Inhibition of complex-type glycosylation with kifunensine, an inhibitor of the oligosaccharide processing enzyme mannosidase 1, had no effect on either the cell-surface expression or antiviral activity of tetherin.
29303997	8	64	contain	had	1455:1457	arg2	effect					1462:1467	no effect	1459:1467	no effect on either the cell-surface expression or antiviral activity of tetherin	1459:1539	Inhibition of complex-type glycosylation with kifunensine, an inhibitor of the oligosaccharide processing enzyme mannosidase 1, had no effect on either the cell-surface expression or antiviral activity of tetherin.
29303997	9	65	theme	single	1604:1609	arg1	residue					1622:1628	a single asparagine residue	1602:1628	a single asparagine residue	1602:1628	These results demonstrate that high-mannose modification of a single asparagine residue is necessary and sufficient, while complex-type glycosylation is dispensable, for cell-surface tetherin expression and antiviral activity.
29303997	4	66	theme	type	876:879	arg1	release					822:828	the release	818:828	the release of Vpu-defective human immunodeficiency virus type 1 (HIV-1)	818:889	The single N-linked glycosylation mutants, N65A and N92A, efficiently inhibited the release of Vpu-defective human immunodeficiency virus type 1 (HIV-1).
29303997	4	67	theme	single	742:747	arg1	N92A					790:793	N92A	790:793	N92A	790:793	The single N-linked glycosylation mutants, N65A and N92A, efficiently inhibited the release of Vpu-defective human immunodeficiency virus type 1 (HIV-1).
29303997	4	67	theme	single	742:747	arg1	N65A					781:784	N65A	781:784	N65A	781:784	The single N-linked glycosylation mutants, N65A and N92A, efficiently inhibited the release of Vpu-defective human immunodeficiency virus type 1 (HIV-1).
29303997	4	67	theme	single	742:747	arg1	mutants					772:778	The single N-linked glycosylation mutants	738:778	The single N-linked glycosylation mutants	738:778	The single N-linked glycosylation mutants, N65A and N92A, efficiently inhibited the release of Vpu-defective human immunodeficiency virus type 1 (HIV-1).
29303997	0	68	gly	Glycosylation	34:46	arg1	Tetherin					51:58	Tetherin	51:58	Tetherin	51:58	High-Mannose But Not Complex-Type Glycosylation of Tetherin Is Required for Restriction of HIV-1 Release.
29303997	8	69	theme	glycosylation	1354:1366	arg1	Inhibition					1327:1336	Inhibition	1327:1336	Inhibition of complex-type glycosylation with kifunensine, an inhibitor of the oligosaccharide processing enzyme mannosidase 1,	1327:1453	Inhibition of complex-type glycosylation with kifunensine, an inhibitor of the oligosaccharide processing enzyme mannosidase 1, had no effect on either the cell-surface expression or antiviral activity of tetherin.
29303997	1	70	theme	interferon-inducible	121:140	arg1	protein					152:158	an interferon-inducible antiviral protein	118:158	an interferon-inducible antiviral protein that inhibits the release of a broad spectrum of enveloped viruses by retaining virions at the surface of infected cells	118:279	Tetherin is an interferon-inducible antiviral protein that inhibits the release of a broad spectrum of enveloped viruses by retaining virions at the surface of infected cells.
29303997	1	70	theme	interferon-inducible	121:140	arg1	Tetherin					106:113	Tetherin	106:113	Tetherin	106:113	Tetherin is an interferon-inducible antiviral protein that inhibits the release of a broad spectrum of enveloped viruses by retaining virions at the surface of infected cells.
29303997	4	71	theme	virus	870:874	arg1	type					876:879	Vpu-defective human immunodeficiency virus type 1	833:881	Vpu-defective human immunodeficiency virus type 1 (HIV-1)	833:889	The single N-linked glycosylation mutants, N65A and N92A, efficiently inhibited the release of Vpu-defective human immunodeficiency virus type 1 (HIV-1).
29303997	4	71	theme	virus	870:874	arg1	HIV-1					884:888	HIV-1	884:888	HIV-1	884:888	The single N-linked glycosylation mutants, N65A and N92A, efficiently inhibited the release of Vpu-defective human immunodeficiency virus type 1 (HIV-1).
29303997	7	72	theme	first	1225:1229	arg1	step					1231:1234	the first step	1221:1234	the first step of N-linked glycosylation	1221:1260	A role for glycosylation in cell-surface tetherin expression is supported by tunicamycin treatment, which inhibits the first step of N-linked glycosylation and impairs both cell-surface expression and antiviral activity.
29303997	7	73	theme	antiviral	1307:1315	arg1	activity					1317:1324	antiviral activity	1307:1324	antiviral activity	1307:1324	A role for glycosylation in cell-surface tetherin expression is supported by tunicamycin treatment, which inhibits the first step of N-linked glycosylation and impairs both cell-surface expression and antiviral activity.
29303997	2	74	theme	domains	318:324	arg1	role					292:295	the role	288:295	the role of specific tetherin domains in antiviral activity	288:346	While the role of specific tetherin domains in antiviral activity is clearly established, the role of glycosylation in tetherin function is not clear.
29303997	5	75	gly	non-glycosylated	909:924	arg1	N65,92A					941:947	N65,92A	941:947	N65,92A	941:947	In contrast, the non-glycosylated double mutant, N65,92A, lost its ability to block HIV-1 release.
29303997	5	75	gly	non-glycosylated	909:924	arg1	mutant					933:938	the non-glycosylated double mutant	905:938	the non-glycosylated double mutant	905:938	In contrast, the non-glycosylated double mutant, N65,92A, lost its ability to block HIV-1 release.
29303997	3	76	theme	glycosylation	571:583	arg1	one					541:543	one	541:543	one	541:543	In this study, we carried out a detailed investigation of this question by using tetherin variants in which one or both sites of N-linked glycosylation were mutated (N65A, N92A, and N65,92A), and chemical inhibitors that prevent glycosylation at specific stages of oligosaccharide were added or modified.
29303997	3	76	theme	glycosylation	571:583	arg1	glycosylation					571:583	N-linked glycosylation	562:583	N-linked glycosylation	562:583	In this study, we carried out a detailed investigation of this question by using tetherin variants in which one or both sites of N-linked glycosylation were mutated (N65A, N92A, and N65,92A), and chemical inhibitors that prevent glycosylation at specific stages of oligosaccharide were added or modified.
29303997	7	77	theme	N-linked	1239:1246	arg1	glycosylation					1248:1260	N-linked glycosylation	1239:1260	N-linked glycosylation	1239:1260	A role for glycosylation in cell-surface tetherin expression is supported by tunicamycin treatment, which inhibits the first step of N-linked glycosylation and impairs both cell-surface expression and antiviral activity.
29303997	6	78	theme	HIV-1	1038:1042	arg1	release					1044:1050	HIV-1 release	1038:1050	HIV-1 release	1038:1050	The inability of the N65,92A mutant to inhibit HIV-1 release is associated with a lack of cell-surface expression.
29303997	8	79	with	Inhibition	1327:1336	arg1	kifunensine					1373:1383	kifunensine	1373:1383	kifunensine	1373:1383	Inhibition of complex-type glycosylation with kifunensine, an inhibitor of the oligosaccharide processing enzyme mannosidase 1, had no effect on either the cell-surface expression or antiviral activity of tetherin.
29303997	8	79	with	Inhibition	1327:1336	arg1	inhibitor					1389:1397	an inhibitor	1386:1397	an inhibitor of the oligosaccharide processing enzyme mannosidase 1	1386:1452	Inhibition of complex-type glycosylation with kifunensine, an inhibitor of the oligosaccharide processing enzyme mannosidase 1, had no effect on either the cell-surface expression or antiviral activity of tetherin.
29303997	4	80	theme	N-linked	749:756	arg1	N92A					790:793	N92A	790:793	N92A	790:793	The single N-linked glycosylation mutants, N65A and N92A, efficiently inhibited the release of Vpu-defective human immunodeficiency virus type 1 (HIV-1).
29303997	4	80	theme	N-linked	749:756	arg1	N65A					781:784	N65A	781:784	N65A	781:784	The single N-linked glycosylation mutants, N65A and N92A, efficiently inhibited the release of Vpu-defective human immunodeficiency virus type 1 (HIV-1).
29303997	4	80	theme	N-linked	749:756	arg1	mutants					772:778	The single N-linked glycosylation mutants	738:778	The single N-linked glycosylation mutants	738:778	The single N-linked glycosylation mutants, N65A and N92A, efficiently inhibited the release of Vpu-defective human immunodeficiency virus type 1 (HIV-1).
29303997	3	81	theme	question	496:503	arg1	investigation					474:486	a detailed investigation	463:486	a detailed investigation of this question	463:503	In this study, we carried out a detailed investigation of this question by using tetherin variants in which one or both sites of N-linked glycosylation were mutated (N65A, N92A, and N65,92A), and chemical inhibitors that prevent glycosylation at specific stages of oligosaccharide were added or modified.
29303997	7	82	theme	cell-surface	1134:1145	arg1	expression					1156:1165	cell-surface tetherin expression	1134:1165	cell-surface tetherin expression	1134:1165	A role for glycosylation in cell-surface tetherin expression is supported by tunicamycin treatment, which inhibits the first step of N-linked glycosylation and impairs both cell-surface expression and antiviral activity.
29687791	3	0	theme	filter	816:821	arg1	enrichment					872:881	enhanced enrichment	863:881	enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment	863:1014	In this study, an enhanced, dedicated, large-scale site-specific quantitative N-glycoproteomics workflow has been established, which includes improved specific extraction of membrane-bound glycoproteins using the filter aided sample preparation (FASP) method, enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment, site-specific N-glycopeptide characterization enabled by EThcD, relative quantitation utilizing isobaric N,N-dimethyl leucine (DiLeu) tags and automated FDR-based large-scale data analysis by Byonic.
29687791	3	0	theme	filter	816:821	arg1	characterization					1046:1061	site-specific N-glycopeptide characterization	1017:1061	site-specific N-glycopeptide characterization	1017:1061	In this study, an enhanced, dedicated, large-scale site-specific quantitative N-glycoproteomics workflow has been established, which includes improved specific extraction of membrane-bound glycoproteins using the filter aided sample preparation (FASP) method, enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment, site-specific N-glycopeptide characterization enabled by EThcD, relative quantitation utilizing isobaric N,N-dimethyl leucine (DiLeu) tags and automated FDR-based large-scale data analysis by Byonic.
29687791	3	0	theme	filter	816:821	arg1	method					855:860	the filter aided sample preparation (FASP) method	812:860	the filter aided sample preparation (FASP) method	812:860	In this study, an enhanced, dedicated, large-scale site-specific quantitative N-glycoproteomics workflow has been established, which includes improved specific extraction of membrane-bound glycoproteins using the filter aided sample preparation (FASP) method, enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment, site-specific N-glycopeptide characterization enabled by EThcD, relative quantitation utilizing isobaric N,N-dimethyl leucine (DiLeu) tags and automated FDR-based large-scale data analysis by Byonic.
29687791	1	1	theme	exact	301:305	arg1	relevance					318:326	the exact functional relevance	297:326	the exact functional relevance of protein glycosylation	297:351	The system-wide site-specific analysis of intact glycopeptides is crucial for understanding the exact functional relevance of protein glycosylation.
29687791	10	2	theme	signaling	2502:2510	arg1	pathways					2512:2519	signaling pathways	2502:2519	signaling pathways	2502:2519	With glycosylation being one of the most important signaling modulators, our results provide additional evidence that signaling pathways are closely regulated by metabolism.
29687791	3	3	theme	FDR-based	1170:1178	arg1	analysis					1197:1204	automated FDR-based large-scale data analysis	1160:1204	automated FDR-based large-scale data analysis	1160:1204	In this study, an enhanced, dedicated, large-scale site-specific quantitative N-glycoproteomics workflow has been established, which includes improved specific extraction of membrane-bound glycoproteins using the filter aided sample preparation (FASP) method, enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment, site-specific N-glycopeptide characterization enabled by EThcD, relative quantitation utilizing isobaric N,N-dimethyl leucine (DiLeu) tags and automated FDR-based large-scale data analysis by Byonic.
29687791	5	4	theme	205	1595:1597	arg1	glycoproteins					1599:1611	205 glycoproteins	1595:1611	205 glycoproteins	1595:1611	When applying the developed workflow to site-specific N-glycoproteome study in PANC1 cells, we were able to identify 1067 intact N-glycopeptides, representing 311 glycosylation sites and 88 glycan compositions from 205 glycoproteins.
29687791	8	5	theme	deglycosylated	2087:2100	arg1	peptides					2102:2109	81 deglycosylated peptides	2084:2109	81 deglycosylated peptides from 70 glycoproteins	2084:2131	A total of 484 deglycosylated peptides were quantified, among which 81 deglycosylated peptides from 70 glycoproteins showed significant changes.
29687791	3	6	theme	enhanced	621:628	arg1	workflow					699:706	an enhanced, dedicated, large-scale site-specific quantitative N-glycoproteomics workflow	618:706	an enhanced, dedicated, large-scale site-specific quantitative N-glycoproteomics workflow	618:706	In this study, an enhanced, dedicated, large-scale site-specific quantitative N-glycoproteomics workflow has been established, which includes improved specific extraction of membrane-bound glycoproteins using the filter aided sample preparation (FASP) method, enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment, site-specific N-glycopeptide characterization enabled by EThcD, relative quantitation utilizing isobaric N,N-dimethyl leucine (DiLeu) tags and automated FDR-based large-scale data analysis by Byonic.
29687791	6	7	theme	knockout	1694:1701	arg1	cells					1703:1707	PKM2 knockout cells	1689:1707	PKM2 knockout cells	1689:1707	We further applied this approach to study the glycosylation alterations in PKM2 knockout cells vs. parental breast cancer cells and revealed altered N-glycoprotein/N-glycopeptide patterns and very different glycosylation microheterogeneity for different types of glycans.
29687791	3	8	theme	N-glycopeptides	886:900	arg1	enrichment					872:881	enhanced enrichment	863:881	enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment	863:1014	In this study, an enhanced, dedicated, large-scale site-specific quantitative N-glycoproteomics workflow has been established, which includes improved specific extraction of membrane-bound glycoproteins using the filter aided sample preparation (FASP) method, enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment, site-specific N-glycopeptide characterization enabled by EThcD, relative quantitation utilizing isobaric N,N-dimethyl leucine (DiLeu) tags and automated FDR-based large-scale data analysis by Byonic.
29687791	3	8	theme	N-glycopeptides	886:900	arg1	method					855:860	the filter aided sample preparation (FASP) method	812:860	the filter aided sample preparation (FASP) method	812:860	In this study, an enhanced, dedicated, large-scale site-specific quantitative N-glycoproteomics workflow has been established, which includes improved specific extraction of membrane-bound glycoproteins using the filter aided sample preparation (FASP) method, enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment, site-specific N-glycopeptide characterization enabled by EThcD, relative quantitation utilizing isobaric N,N-dimethyl leucine (DiLeu) tags and automated FDR-based large-scale data analysis by Byonic.
29687791	3	9	theme	quantitative	668:679	arg1	workflow					699:706	an enhanced, dedicated, large-scale site-specific quantitative N-glycoproteomics workflow	618:706	an enhanced, dedicated, large-scale site-specific quantitative N-glycoproteomics workflow	618:706	In this study, an enhanced, dedicated, large-scale site-specific quantitative N-glycoproteomics workflow has been established, which includes improved specific extraction of membrane-bound glycoproteins using the filter aided sample preparation (FASP) method, enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment, site-specific N-glycopeptide characterization enabled by EThcD, relative quantitation utilizing isobaric N,N-dimethyl leucine (DiLeu) tags and automated FDR-based large-scale data analysis by Byonic.
29687791	1	10	theme	site-specific	221:233	arg1	crucial					271:277	crucial	271:277	crucial	271:277	The system-wide site-specific analysis of intact glycopeptides is crucial for understanding the exact functional relevance of protein glycosylation.
29687791	1	10	theme	site-specific	221:233	arg1	analysis					235:242	The system-wide site-specific analysis	205:242	The system-wide site-specific analysis of intact glycopeptides	205:266	The system-wide site-specific analysis of intact glycopeptides is crucial for understanding the exact functional relevance of protein glycosylation.
29687791	8	11	theme	peptides	2046:2053	arg1	total					2018:2022	A total	2016:2022	A total of 484 deglycosylated peptides	2016:2053	A total of 484 deglycosylated peptides were quantified, among which 81 deglycosylated peptides from 70 glycoproteins showed significant changes.
29687791	3	12	theme	data	1192:1195	arg1	analysis					1197:1204	automated FDR-based large-scale data analysis	1160:1204	automated FDR-based large-scale data analysis	1160:1204	In this study, an enhanced, dedicated, large-scale site-specific quantitative N-glycoproteomics workflow has been established, which includes improved specific extraction of membrane-bound glycoproteins using the filter aided sample preparation (FASP) method, enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment, site-specific N-glycopeptide characterization enabled by EThcD, relative quantitation utilizing isobaric N,N-dimethyl leucine (DiLeu) tags and automated FDR-based large-scale data analysis by Byonic.
29687791	9	13	theme	KEGG	2161:2164	arg1	analysis					2174:2181	KEGG pathway analysis	2161:2181	KEGG pathway analysis	2161:2181	KEGG pathway analysis revealed that the PI3K/Akt signaling pathway was highly enriched, which provided evidence to support the previous finding that PKM2 knockdown cancer cells rely on activation of Akt for their survival.
29687791	5	14	gly	glycosylation	1543:1555	arg2	sites					1557:1561	311 glycosylation sites	1539:1561	311 glycosylation sites	1539:1561	When applying the developed workflow to site-specific N-glycoproteome study in PANC1 cells, we were able to identify 1067 intact N-glycopeptides, representing 311 glycosylation sites and 88 glycan compositions from 205 glycoproteins.
29687791	5	14	gly	glycosylation	1543:1555	arg2	311					1539:1541	311	1539:1541	311	1539:1541	When applying the developed workflow to site-specific N-glycoproteome study in PANC1 cells, we were able to identify 1067 intact N-glycopeptides, representing 311 glycosylation sites and 88 glycan compositions from 205 glycoproteins.
29687791	6	15	theme	glycosylation	1660:1672	arg1	alterations					1674:1684	the glycosylation alterations	1656:1684	the glycosylation alterations in PKM2 knockout cells vs. parental breast cancer cells	1656:1740	We further applied this approach to study the glycosylation alterations in PKM2 knockout cells vs. parental breast cancer cells and revealed altered N-glycoprotein/N-glycopeptide patterns and very different glycosylation microheterogeneity for different types of glycans.
29687791	3	16	theme	sequential	908:917	arg1	chromatography					950:963	sequential hydrophilic interaction liquid chromatography	908:963	sequential hydrophilic interaction liquid chromatography (HILIC)	908:971	In this study, an enhanced, dedicated, large-scale site-specific quantitative N-glycoproteomics workflow has been established, which includes improved specific extraction of membrane-bound glycoproteins using the filter aided sample preparation (FASP) method, enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment, site-specific N-glycopeptide characterization enabled by EThcD, relative quantitation utilizing isobaric N,N-dimethyl leucine (DiLeu) tags and automated FDR-based large-scale data analysis by Byonic.
29687791	3	16	theme	sequential	908:917	arg1	HILIC					966:970	HILIC	966:970	HILIC	966:970	In this study, an enhanced, dedicated, large-scale site-specific quantitative N-glycoproteomics workflow has been established, which includes improved specific extraction of membrane-bound glycoproteins using the filter aided sample preparation (FASP) method, enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment, site-specific N-glycopeptide characterization enabled by EThcD, relative quantitation utilizing isobaric N,N-dimethyl leucine (DiLeu) tags and automated FDR-based large-scale data analysis by Byonic.
29687791	0	17	theme	electron-transfer/higher-energy	141:171	arg1	EThcD					197:201	EThcD	197:201	EThcD	197:201	Site-specific characterization and quantitation of N-glycopeptides in PKM2 knockout breast cancer cells using DiLeu isobaric tags enabled by electron-transfer/higher-energy collision dissociation (EThcD).
29687791	0	17	theme	electron-transfer/higher-energy	141:171	arg1	dissociation					183:194	electron-transfer/higher-energy collision dissociation	141:194	electron-transfer/higher-energy collision dissociation (EThcD)	141:202	Site-specific characterization and quantitation of N-glycopeptides in PKM2 knockout breast cancer cells using DiLeu isobaric tags enabled by electron-transfer/higher-energy collision dissociation (EThcD).
29687791	6	18	theme	N-glycoprotein/N-glycopeptide	1763:1791	arg1	patterns					1793:1800	altered N-glycoprotein/N-glycopeptide patterns	1755:1800	altered N-glycoprotein/N-glycopeptide patterns	1755:1800	We further applied this approach to study the glycosylation alterations in PKM2 knockout cells vs. parental breast cancer cells and revealed altered N-glycoprotein/N-glycopeptide patterns and very different glycosylation microheterogeneity for different types of glycans.
29687791	1	19	theme	glycopeptides	254:266	arg1	crucial					271:277	crucial	271:277	crucial	271:277	The system-wide site-specific analysis of intact glycopeptides is crucial for understanding the exact functional relevance of protein glycosylation.
29687791	1	19	theme	glycopeptides	254:266	arg1	analysis					235:242	The system-wide site-specific analysis	205:242	The system-wide site-specific analysis of intact glycopeptides	205:266	The system-wide site-specific analysis of intact glycopeptides is crucial for understanding the exact functional relevance of protein glycosylation.
29687791	9	20	theme	Akt	2360:2362	arg1	activation					2346:2355	activation	2346:2355	activation of Akt	2346:2362	KEGG pathway analysis revealed that the PI3K/Akt signaling pathway was highly enriched, which provided evidence to support the previous finding that PKM2 knockdown cancer cells rely on activation of Akt for their survival.
29687791	5	21	gly	N-glycopeptides	1509:1523	arg2	N-glycopeptides					1509:1523	1067 intact N-glycopeptides	1497:1523	1067 intact N-glycopeptides	1497:1523	When applying the developed workflow to site-specific N-glycoproteome study in PANC1 cells, we were able to identify 1067 intact N-glycopeptides, representing 311 glycosylation sites and 88 glycan compositions from 205 glycoproteins.
29687791	3	22	dep	enhanced	621:628	arg1	large-scale					642:652	large-scale	642:652	large-scale	642:652	In this study, an enhanced, dedicated, large-scale site-specific quantitative N-glycoproteomics workflow has been established, which includes improved specific extraction of membrane-bound glycoproteins using the filter aided sample preparation (FASP) method, enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment, site-specific N-glycopeptide characterization enabled by EThcD, relative quantitation utilizing isobaric N,N-dimethyl leucine (DiLeu) tags and automated FDR-based large-scale data analysis by Byonic.
29687791	3	22	dep	enhanced	621:628	arg1	dedicated					631:639	dedicated	631:639	dedicated	631:639	In this study, an enhanced, dedicated, large-scale site-specific quantitative N-glycoproteomics workflow has been established, which includes improved specific extraction of membrane-bound glycoproteins using the filter aided sample preparation (FASP) method, enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment, site-specific N-glycopeptide characterization enabled by EThcD, relative quantitation utilizing isobaric N,N-dimethyl leucine (DiLeu) tags and automated FDR-based large-scale data analysis by Byonic.
29687791	3	23	theme	MLA	1000:1002	arg1	enrichment					1005:1014	multi-lectin affinity (MLA) enrichment	977:1014	multi-lectin affinity (MLA) enrichment	977:1014	In this study, an enhanced, dedicated, large-scale site-specific quantitative N-glycoproteomics workflow has been established, which includes improved specific extraction of membrane-bound glycoproteins using the filter aided sample preparation (FASP) method, enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment, site-specific N-glycopeptide characterization enabled by EThcD, relative quantitation utilizing isobaric N,N-dimethyl leucine (DiLeu) tags and automated FDR-based large-scale data analysis by Byonic.
29687791	6	24	theme	glycans	1877:1883	arg1	types					1868:1872	different types	1858:1872	different types of glycans	1858:1883	We further applied this approach to study the glycosylation alterations in PKM2 knockout cells vs. parental breast cancer cells and revealed altered N-glycoprotein/N-glycopeptide patterns and very different glycosylation microheterogeneity for different types of glycans.
29687791	9	25	theme	PI3K/Akt	2201:2208	arg1	pathway					2220:2226	the PI3K/Akt signaling pathway	2197:2226	the PI3K/Akt signaling pathway	2197:2226	KEGG pathway analysis revealed that the PI3K/Akt signaling pathway was highly enriched, which provided evidence to support the previous finding that PKM2 knockdown cancer cells rely on activation of Akt for their survival.
29687791	5	26	theme	site-specific	1420:1432	arg1	study					1450:1454	site-specific N-glycoproteome study	1420:1454	site-specific N-glycoproteome study	1420:1454	When applying the developed workflow to site-specific N-glycoproteome study in PANC1 cells, we were able to identify 1067 intact N-glycopeptides, representing 311 glycosylation sites and 88 glycan compositions from 205 glycoproteins.
29687791	3	27	theme	affinity	990:997	arg1	enrichment					1005:1014	multi-lectin affinity (MLA) enrichment	977:1014	multi-lectin affinity (MLA) enrichment	977:1014	In this study, an enhanced, dedicated, large-scale site-specific quantitative N-glycoproteomics workflow has been established, which includes improved specific extraction of membrane-bound glycoproteins using the filter aided sample preparation (FASP) method, enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment, site-specific N-glycopeptide characterization enabled by EThcD, relative quantitation utilizing isobaric N,N-dimethyl leucine (DiLeu) tags and automated FDR-based large-scale data analysis by Byonic.
29687791	4	28	theme	large	1286:1290	arg1	extent					1292:1297	a very large extent	1279:1297	a very large extent to MLA enrichment with only 20% overlapping in enriching intact N-glycopeptides	1279:1377	For the first time, our study shows that HILIC complements to a very large extent to MLA enrichment with only 20% overlapping in enriching intact N-glycopeptides.
29687791	5	29	gly	glycoproteins	1599:1611	arg1	glycoproteins					1599:1611	205 glycoproteins	1595:1611	205 glycoproteins	1595:1611	When applying the developed workflow to site-specific N-glycoproteome study in PANC1 cells, we were able to identify 1067 intact N-glycopeptides, representing 311 glycosylation sites and 88 glycan compositions from 205 glycoproteins.
29687791	0	30	theme	knockout	75:82	arg1	cells					98:102	PKM2 knockout breast cancer cells	70:102	PKM2 knockout breast cancer cells using DiLeu isobaric tags	70:128	Site-specific characterization and quantitation of N-glycopeptides in PKM2 knockout breast cancer cells using DiLeu isobaric tags enabled by electron-transfer/higher-energy collision dissociation (EThcD).
29687791	6	31	theme	breast	1722:1727	arg1	cells					1736:1740	parental breast cancer cells	1713:1740	parental breast cancer cells	1713:1740	We further applied this approach to study the glycosylation alterations in PKM2 knockout cells vs. parental breast cancer cells and revealed altered N-glycoprotein/N-glycopeptide patterns and very different glycosylation microheterogeneity for different types of glycans.
29687791	3	32	theme	site-specific	1017:1029	arg1	characterization					1046:1061	site-specific N-glycopeptide characterization	1017:1061	site-specific N-glycopeptide characterization	1017:1061	In this study, an enhanced, dedicated, large-scale site-specific quantitative N-glycoproteomics workflow has been established, which includes improved specific extraction of membrane-bound glycoproteins using the filter aided sample preparation (FASP) method, enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment, site-specific N-glycopeptide characterization enabled by EThcD, relative quantitation utilizing isobaric N,N-dimethyl leucine (DiLeu) tags and automated FDR-based large-scale data analysis by Byonic.
29687791	3	32	theme	site-specific	1017:1029	arg1	method					855:860	the filter aided sample preparation (FASP) method	812:860	the filter aided sample preparation (FASP) method	812:860	In this study, an enhanced, dedicated, large-scale site-specific quantitative N-glycoproteomics workflow has been established, which includes improved specific extraction of membrane-bound glycoproteins using the filter aided sample preparation (FASP) method, enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment, site-specific N-glycopeptide characterization enabled by EThcD, relative quantitation utilizing isobaric N,N-dimethyl leucine (DiLeu) tags and automated FDR-based large-scale data analysis by Byonic.
29687791	2	33	theme	biological	583:592	arg1	systems					594:600	complex biological systems	575:600	complex biological systems	575:600	A dedicated workflow with the capability to simultaneously characterize and quantify intact glycopeptides in a site-specific and high-throughput manner is essential to reveal specific glycosylation alteration patterns in complex biological systems.
29687791	0	34	theme	cancer	91:96	arg1	cells					98:102	PKM2 knockout breast cancer cells	70:102	PKM2 knockout breast cancer cells using DiLeu isobaric tags	70:128	Site-specific characterization and quantitation of N-glycopeptides in PKM2 knockout breast cancer cells using DiLeu isobaric tags enabled by electron-transfer/higher-energy collision dissociation (EThcD).
29687791	6	35	theme	different	1858:1866	arg1	types					1868:1872	different types	1858:1872	different types of glycans	1858:1883	We further applied this approach to study the glycosylation alterations in PKM2 knockout cells vs. parental breast cancer cells and revealed altered N-glycoprotein/N-glycopeptide patterns and very different glycosylation microheterogeneity for different types of glycans.
29687791	7	36	theme	comprehensive	1903:1915	arg1	map					1917:1919	a more comprehensive map	1896:1919	a more comprehensive map of glycoprotein alterations	1896:1947	To obtain a more comprehensive map of glycoprotein alterations, N-glycopeptides after treatment with PNGase F were also analyzed.
29687791	3	37	gly	N-glycopeptides	886:900	arg2	N-glycopeptides					886:900	N-glycopeptides	886:900	N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment	886:1014	In this study, an enhanced, dedicated, large-scale site-specific quantitative N-glycoproteomics workflow has been established, which includes improved specific extraction of membrane-bound glycoproteins using the filter aided sample preparation (FASP) method, enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment, site-specific N-glycopeptide characterization enabled by EThcD, relative quantitation utilizing isobaric N,N-dimethyl leucine (DiLeu) tags and automated FDR-based large-scale data analysis by Byonic.
29687791	4	38	theme	intact	1356:1361	arg1	N-glycopeptides					1363:1377	intact N-glycopeptides	1356:1377	intact N-glycopeptides	1356:1377	For the first time, our study shows that HILIC complements to a very large extent to MLA enrichment with only 20% overlapping in enriching intact N-glycopeptides.
29687791	10	39	theme	important	2425:2433	arg1	modulators					2445:2454	the most important signaling modulators	2416:2454	the most important signaling modulators	2416:2454	With glycosylation being one of the most important signaling modulators, our results provide additional evidence that signaling pathways are closely regulated by metabolism.
29687791	2	40	with	workflow	366:373	arg1	capability					384:393	the capability to simultaneously characterize and quantify intact glycopeptides in a site-specific and high-throughput manner	380:504	the capability to simultaneously characterize and quantify intact glycopeptides in a site-specific and high-throughput manner	380:504	A dedicated workflow with the capability to simultaneously characterize and quantify intact glycopeptides in a site-specific and high-throughput manner is essential to reveal specific glycosylation alteration patterns in complex biological systems.
29687791	10	41	theme	signaling	2435:2443	arg1	modulators					2445:2454	the most important signaling modulators	2416:2454	the most important signaling modulators	2416:2454	With glycosylation being one of the most important signaling modulators, our results provide additional evidence that signaling pathways are closely regulated by metabolism.
29687791	7	42	theme	alterations	1937:1947	arg1	map					1917:1919	a more comprehensive map	1896:1919	a more comprehensive map of glycoprotein alterations	1896:1947	To obtain a more comprehensive map of glycoprotein alterations, N-glycopeptides after treatment with PNGase F were also analyzed.
29687791	2	43	theme	site-specific	465:477	arg1	manner					499:504	a site-specific and high-throughput manner	463:504	a site-specific and high-throughput manner	463:504	A dedicated workflow with the capability to simultaneously characterize and quantify intact glycopeptides in a site-specific and high-throughput manner is essential to reveal specific glycosylation alteration patterns in complex biological systems.
29687791	0	44	theme	isobaric	116:123	arg1	tags					125:128	DiLeu isobaric tags	110:128	DiLeu isobaric tags	110:128	Site-specific characterization and quantitation of N-glycopeptides in PKM2 knockout breast cancer cells using DiLeu isobaric tags enabled by electron-transfer/higher-energy collision dissociation (EThcD).
29687791	6	45	theme	different	1811:1819	arg1	microheterogeneity					1835:1852	very different glycosylation microheterogeneity	1806:1852	very different glycosylation microheterogeneity	1806:1852	We further applied this approach to study the glycosylation alterations in PKM2 knockout cells vs. parental breast cancer cells and revealed altered N-glycoprotein/N-glycopeptide patterns and very different glycosylation microheterogeneity for different types of glycans.
29687791	2	46	theme	specific	529:536	arg1	patterns					563:570	specific glycosylation alteration patterns	529:570	specific glycosylation alteration patterns in complex biological systems	529:600	A dedicated workflow with the capability to simultaneously characterize and quantify intact glycopeptides in a site-specific and high-throughput manner is essential to reveal specific glycosylation alteration patterns in complex biological systems.
29687791	2	47	from	patterns	563:570	arg1	systems					594:600	complex biological systems	575:600	complex biological systems	575:600	A dedicated workflow with the capability to simultaneously characterize and quantify intact glycopeptides in a site-specific and high-throughput manner is essential to reveal specific glycosylation alteration patterns in complex biological systems.
29687791	2	48	gly	glycopeptides	446:458	arg2	glycopeptides					446:458	intact glycopeptides	439:458	intact glycopeptides	439:458	A dedicated workflow with the capability to simultaneously characterize and quantify intact glycopeptides in a site-specific and high-throughput manner is essential to reveal specific glycosylation alteration patterns in complex biological systems.
29687791	10	49	theme	additional	2477:2486	arg1	evidence					2488:2495	additional evidence that signaling pathways are closely regulated by metabolism	2477:2555	additional evidence that signaling pathways are closely regulated by metabolism	2477:2555	With glycosylation being one of the most important signaling modulators, our results provide additional evidence that signaling pathways are closely regulated by metabolism.
29687791	0	50	theme	Site-specific	0:12	arg1	characterization					14:29	Site-specific characterization	0:29	Site-specific characterization	0:29	Site-specific characterization and quantitation of N-glycopeptides in PKM2 knockout breast cancer cells using DiLeu isobaric tags enabled by electron-transfer/higher-energy collision dissociation (EThcD).
29687791	5	51	theme	PANC1	1459:1463	arg1	cells					1465:1469	PANC1 cells	1459:1469	PANC1 cells	1459:1469	When applying the developed workflow to site-specific N-glycoproteome study in PANC1 cells, we were able to identify 1067 intact N-glycopeptides, representing 311 glycosylation sites and 88 glycan compositions from 205 glycoproteins.
29687791	1	52	theme	glycosylation	339:351	arg1	relevance					318:326	the exact functional relevance	297:326	the exact functional relevance of protein glycosylation	297:351	The system-wide site-specific analysis of intact glycopeptides is crucial for understanding the exact functional relevance of protein glycosylation.
29687791	7	53	gly	glycoprotein	1924:1935	arg1	glycoprotein					1924:1935	glycoprotein alterations	1924:1947	glycoprotein alterations	1924:1947	To obtain a more comprehensive map of glycoprotein alterations, N-glycopeptides after treatment with PNGase F were also analyzed.
29687791	6	54	from	alterations	1674:1684	arg1	cells					1736:1740	parental breast cancer cells	1713:1740	parental breast cancer cells	1713:1740	We further applied this approach to study the glycosylation alterations in PKM2 knockout cells vs. parental breast cancer cells and revealed altered N-glycoprotein/N-glycopeptide patterns and very different glycosylation microheterogeneity for different types of glycans.
29687791	6	54	from	alterations	1674:1684	arg1	cells					1703:1707	PKM2 knockout cells	1689:1707	PKM2 knockout cells	1689:1707	We further applied this approach to study the glycosylation alterations in PKM2 knockout cells vs. parental breast cancer cells and revealed altered N-glycoprotein/N-glycopeptide patterns and very different glycosylation microheterogeneity for different types of glycans.
29687791	8	55	gly	deglycosylated	2031:2044	arg1	peptides					2046:2053	484 deglycosylated peptides	2027:2053	484 deglycosylated peptides	2027:2053	A total of 484 deglycosylated peptides were quantified, among which 81 deglycosylated peptides from 70 glycoproteins showed significant changes.
29687791	2	56	theme	high-throughput	483:497	arg1	manner					499:504	a site-specific and high-throughput manner	463:504	a site-specific and high-throughput manner	463:504	A dedicated workflow with the capability to simultaneously characterize and quantify intact glycopeptides in a site-specific and high-throughput manner is essential to reveal specific glycosylation alteration patterns in complex biological systems.
29687791	3	57	theme	interaction	931:941	arg1	chromatography					950:963	sequential hydrophilic interaction liquid chromatography	908:963	sequential hydrophilic interaction liquid chromatography (HILIC)	908:971	In this study, an enhanced, dedicated, large-scale site-specific quantitative N-glycoproteomics workflow has been established, which includes improved specific extraction of membrane-bound glycoproteins using the filter aided sample preparation (FASP) method, enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment, site-specific N-glycopeptide characterization enabled by EThcD, relative quantitation utilizing isobaric N,N-dimethyl leucine (DiLeu) tags and automated FDR-based large-scale data analysis by Byonic.
29687791	3	57	theme	interaction	931:941	arg1	HILIC					966:970	HILIC	966:970	HILIC	966:970	In this study, an enhanced, dedicated, large-scale site-specific quantitative N-glycoproteomics workflow has been established, which includes improved specific extraction of membrane-bound glycoproteins using the filter aided sample preparation (FASP) method, enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment, site-specific N-glycopeptide characterization enabled by EThcD, relative quantitation utilizing isobaric N,N-dimethyl leucine (DiLeu) tags and automated FDR-based large-scale data analysis by Byonic.
29687791	2	58	theme	alteration	552:561	arg1	patterns					563:570	specific glycosylation alteration patterns	529:570	specific glycosylation alteration patterns in complex biological systems	529:600	A dedicated workflow with the capability to simultaneously characterize and quantify intact glycopeptides in a site-specific and high-throughput manner is essential to reveal specific glycosylation alteration patterns in complex biological systems.
29687791	3	59	theme	specific	754:761	arg1	extraction					763:772	improved specific extraction	745:772	improved specific extraction of membrane-bound glycoproteins using the filter aided sample preparation (FASP) method, enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment, site-specific N-glycopeptide characterization enabled by EThcD, relative quantitation utilizing isobaric N,N-dimethyl leucine (DiLeu) tags and automated FDR-based large-scale data analysis by Byonic	745:1214	In this study, an enhanced, dedicated, large-scale site-specific quantitative N-glycoproteomics workflow has been established, which includes improved specific extraction of membrane-bound glycoproteins using the filter aided sample preparation (FASP) method, enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment, site-specific N-glycopeptide characterization enabled by EThcD, relative quantitation utilizing isobaric N,N-dimethyl leucine (DiLeu) tags and automated FDR-based large-scale data analysis by Byonic.
29687791	3	60	theme	isobaric	1113:1120	arg1	N					1122:1122	isobaric N	1113:1122	isobaric N	1113:1122	In this study, an enhanced, dedicated, large-scale site-specific quantitative N-glycoproteomics workflow has been established, which includes improved specific extraction of membrane-bound glycoproteins using the filter aided sample preparation (FASP) method, enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment, site-specific N-glycopeptide characterization enabled by EThcD, relative quantitation utilizing isobaric N,N-dimethyl leucine (DiLeu) tags and automated FDR-based large-scale data analysis by Byonic.
29687791	9	61	theme	cancer	2325:2330	arg1	cells					2332:2336	PKM2 knockdown cancer cells	2310:2336	PKM2 knockdown cancer cells	2310:2336	KEGG pathway analysis revealed that the PI3K/Akt signaling pathway was highly enriched, which provided evidence to support the previous finding that PKM2 knockdown cancer cells rely on activation of Akt for their survival.
29687791	5	62	theme	glycosylation	1543:1555	arg1	sites					1557:1561	311 glycosylation sites	1539:1561	311 glycosylation sites	1539:1561	When applying the developed workflow to site-specific N-glycoproteome study in PANC1 cells, we were able to identify 1067 intact N-glycopeptides, representing 311 glycosylation sites and 88 glycan compositions from 205 glycoproteins.
29687791	0	63	gly	N-glycopeptides	51:65	arg2	N-glycopeptides					51:65	N-glycopeptides	51:65	N-glycopeptides	51:65	Site-specific characterization and quantitation of N-glycopeptides in PKM2 knockout breast cancer cells using DiLeu isobaric tags enabled by electron-transfer/higher-energy collision dissociation (EThcD).
29687791	3	64	theme	DiLeu	1144:1148	arg1	tags					1151:1154	N-dimethyl leucine (DiLeu) tags	1124:1154	N-dimethyl leucine (DiLeu) tags	1124:1154	In this study, an enhanced, dedicated, large-scale site-specific quantitative N-glycoproteomics workflow has been established, which includes improved specific extraction of membrane-bound glycoproteins using the filter aided sample preparation (FASP) method, enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment, site-specific N-glycopeptide characterization enabled by EThcD, relative quantitation utilizing isobaric N,N-dimethyl leucine (DiLeu) tags and automated FDR-based large-scale data analysis by Byonic.
29687791	3	65	theme	glycoproteins	792:804	arg1	extraction					763:772	improved specific extraction	745:772	improved specific extraction of membrane-bound glycoproteins using the filter aided sample preparation (FASP) method, enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment, site-specific N-glycopeptide characterization enabled by EThcD, relative quantitation utilizing isobaric N,N-dimethyl leucine (DiLeu) tags and automated FDR-based large-scale data analysis by Byonic	745:1214	In this study, an enhanced, dedicated, large-scale site-specific quantitative N-glycoproteomics workflow has been established, which includes improved specific extraction of membrane-bound glycoproteins using the filter aided sample preparation (FASP) method, enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment, site-specific N-glycopeptide characterization enabled by EThcD, relative quantitation utilizing isobaric N,N-dimethyl leucine (DiLeu) tags and automated FDR-based large-scale data analysis by Byonic.
29687791	2	66	theme	intact	439:444	arg1	glycopeptides					446:458	intact glycopeptides	439:458	intact glycopeptides	439:458	A dedicated workflow with the capability to simultaneously characterize and quantify intact glycopeptides in a site-specific and high-throughput manner is essential to reveal specific glycosylation alteration patterns in complex biological systems.
29687791	3	67	theme	leucine	1135:1141	arg1	tags					1151:1154	N-dimethyl leucine (DiLeu) tags	1124:1154	N-dimethyl leucine (DiLeu) tags	1124:1154	In this study, an enhanced, dedicated, large-scale site-specific quantitative N-glycoproteomics workflow has been established, which includes improved specific extraction of membrane-bound glycoproteins using the filter aided sample preparation (FASP) method, enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment, site-specific N-glycopeptide characterization enabled by EThcD, relative quantitation utilizing isobaric N,N-dimethyl leucine (DiLeu) tags and automated FDR-based large-scale data analysis by Byonic.
29687791	5	68	from	glycoproteins	1599:1611	arg1	sites					1557:1561	311 glycosylation sites	1539:1561	311 glycosylation sites	1539:1561	When applying the developed workflow to site-specific N-glycoproteome study in PANC1 cells, we were able to identify 1067 intact N-glycopeptides, representing 311 glycosylation sites and 88 glycan compositions from 205 glycoproteins.
29687791	5	68	from	glycoproteins	1599:1611	arg1	compositions					1577:1588	88 glycan compositions	1567:1588	88 glycan compositions from 205 glycoproteins	1567:1611	When applying the developed workflow to site-specific N-glycoproteome study in PANC1 cells, we were able to identify 1067 intact N-glycopeptides, representing 311 glycosylation sites and 88 glycan compositions from 205 glycoproteins.
29687791	8	69	gly	deglycosylated	2087:2100	arg1	peptides					2102:2109	81 deglycosylated peptides	2084:2109	81 deglycosylated peptides from 70 glycoproteins	2084:2131	A total of 484 deglycosylated peptides were quantified, among which 81 deglycosylated peptides from 70 glycoproteins showed significant changes.
29687791	8	70	from	glycoproteins	2119:2131	arg1	peptides					2102:2109	81 deglycosylated peptides	2084:2109	81 deglycosylated peptides from 70 glycoproteins	2084:2131	A total of 484 deglycosylated peptides were quantified, among which 81 deglycosylated peptides from 70 glycoproteins showed significant changes.
29687791	3	71	theme	preparation	836:846	arg1	enrichment					872:881	enhanced enrichment	863:881	enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment	863:1014	In this study, an enhanced, dedicated, large-scale site-specific quantitative N-glycoproteomics workflow has been established, which includes improved specific extraction of membrane-bound glycoproteins using the filter aided sample preparation (FASP) method, enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment, site-specific N-glycopeptide characterization enabled by EThcD, relative quantitation utilizing isobaric N,N-dimethyl leucine (DiLeu) tags and automated FDR-based large-scale data analysis by Byonic.
29687791	3	71	theme	preparation	836:846	arg1	characterization					1046:1061	site-specific N-glycopeptide characterization	1017:1061	site-specific N-glycopeptide characterization	1017:1061	In this study, an enhanced, dedicated, large-scale site-specific quantitative N-glycoproteomics workflow has been established, which includes improved specific extraction of membrane-bound glycoproteins using the filter aided sample preparation (FASP) method, enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment, site-specific N-glycopeptide characterization enabled by EThcD, relative quantitation utilizing isobaric N,N-dimethyl leucine (DiLeu) tags and automated FDR-based large-scale data analysis by Byonic.
29687791	3	71	theme	preparation	836:846	arg1	method					855:860	the filter aided sample preparation (FASP) method	812:860	the filter aided sample preparation (FASP) method	812:860	In this study, an enhanced, dedicated, large-scale site-specific quantitative N-glycoproteomics workflow has been established, which includes improved specific extraction of membrane-bound glycoproteins using the filter aided sample preparation (FASP) method, enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment, site-specific N-glycopeptide characterization enabled by EThcD, relative quantitation utilizing isobaric N,N-dimethyl leucine (DiLeu) tags and automated FDR-based large-scale data analysis by Byonic.
29687791	4	72	with	enrichment	1306:1315	arg1	%					1329:1329	only 20%	1322:1329	only 20% overlapping in enriching intact N-glycopeptides	1322:1377	For the first time, our study shows that HILIC complements to a very large extent to MLA enrichment with only 20% overlapping in enriching intact N-glycopeptides.
29687791	3	73	theme	automated	1160:1168	arg1	analysis					1197:1204	automated FDR-based large-scale data analysis	1160:1204	automated FDR-based large-scale data analysis	1160:1204	In this study, an enhanced, dedicated, large-scale site-specific quantitative N-glycoproteomics workflow has been established, which includes improved specific extraction of membrane-bound glycoproteins using the filter aided sample preparation (FASP) method, enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment, site-specific N-glycopeptide characterization enabled by EThcD, relative quantitation utilizing isobaric N,N-dimethyl leucine (DiLeu) tags and automated FDR-based large-scale data analysis by Byonic.
29687791	9	74	theme	PKM2	2310:2313	arg1	cells					2332:2336	PKM2 knockdown cancer cells	2310:2336	PKM2 knockdown cancer cells	2310:2336	KEGG pathway analysis revealed that the PI3K/Akt signaling pathway was highly enriched, which provided evidence to support the previous finding that PKM2 knockdown cancer cells rely on activation of Akt for their survival.
29687791	3	75	theme	aided	823:827	arg1	enrichment					872:881	enhanced enrichment	863:881	enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment	863:1014	In this study, an enhanced, dedicated, large-scale site-specific quantitative N-glycoproteomics workflow has been established, which includes improved specific extraction of membrane-bound glycoproteins using the filter aided sample preparation (FASP) method, enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment, site-specific N-glycopeptide characterization enabled by EThcD, relative quantitation utilizing isobaric N,N-dimethyl leucine (DiLeu) tags and automated FDR-based large-scale data analysis by Byonic.
29687791	3	75	theme	aided	823:827	arg1	characterization					1046:1061	site-specific N-glycopeptide characterization	1017:1061	site-specific N-glycopeptide characterization	1017:1061	In this study, an enhanced, dedicated, large-scale site-specific quantitative N-glycoproteomics workflow has been established, which includes improved specific extraction of membrane-bound glycoproteins using the filter aided sample preparation (FASP) method, enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment, site-specific N-glycopeptide characterization enabled by EThcD, relative quantitation utilizing isobaric N,N-dimethyl leucine (DiLeu) tags and automated FDR-based large-scale data analysis by Byonic.
29687791	3	75	theme	aided	823:827	arg1	method					855:860	the filter aided sample preparation (FASP) method	812:860	the filter aided sample preparation (FASP) method	812:860	In this study, an enhanced, dedicated, large-scale site-specific quantitative N-glycoproteomics workflow has been established, which includes improved specific extraction of membrane-bound glycoproteins using the filter aided sample preparation (FASP) method, enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment, site-specific N-glycopeptide characterization enabled by EThcD, relative quantitation utilizing isobaric N,N-dimethyl leucine (DiLeu) tags and automated FDR-based large-scale data analysis by Byonic.
29687791	1	76	theme	functional	307:316	arg1	relevance					318:326	the exact functional relevance	297:326	the exact functional relevance of protein glycosylation	297:351	The system-wide site-specific analysis of intact glycopeptides is crucial for understanding the exact functional relevance of protein glycosylation.
29687791	5	77	from	workflow	1408:1415	arg1	cells					1465:1469	PANC1 cells	1459:1469	PANC1 cells	1459:1469	When applying the developed workflow to site-specific N-glycoproteome study in PANC1 cells, we were able to identify 1067 intact N-glycopeptides, representing 311 glycosylation sites and 88 glycan compositions from 205 glycoproteins.
29687791	8	78	gly	glycoproteins	2119:2131	arg1	glycoproteins					2119:2131	70 glycoproteins	2116:2131	70 glycoproteins	2116:2131	A total of 484 deglycosylated peptides were quantified, among which 81 deglycosylated peptides from 70 glycoproteins showed significant changes.
29687791	7	79	theme	glycoprotein	1924:1935	arg1	alterations					1937:1947	glycoprotein alterations	1924:1947	glycoprotein alterations	1924:1947	To obtain a more comprehensive map of glycoprotein alterations, N-glycopeptides after treatment with PNGase F were also analyzed.
29687791	7	80	theme	PNGase	1987:1992	arg1	F					1994:1994	PNGase F	1987:1994	PNGase F	1987:1994	To obtain a more comprehensive map of glycoprotein alterations, N-glycopeptides after treatment with PNGase F were also analyzed.
29687791	3	81	theme	FASP	849:852	arg1	enrichment					872:881	enhanced enrichment	863:881	enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment	863:1014	In this study, an enhanced, dedicated, large-scale site-specific quantitative N-glycoproteomics workflow has been established, which includes improved specific extraction of membrane-bound glycoproteins using the filter aided sample preparation (FASP) method, enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment, site-specific N-glycopeptide characterization enabled by EThcD, relative quantitation utilizing isobaric N,N-dimethyl leucine (DiLeu) tags and automated FDR-based large-scale data analysis by Byonic.
29687791	3	81	theme	FASP	849:852	arg1	characterization					1046:1061	site-specific N-glycopeptide characterization	1017:1061	site-specific N-glycopeptide characterization	1017:1061	In this study, an enhanced, dedicated, large-scale site-specific quantitative N-glycoproteomics workflow has been established, which includes improved specific extraction of membrane-bound glycoproteins using the filter aided sample preparation (FASP) method, enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment, site-specific N-glycopeptide characterization enabled by EThcD, relative quantitation utilizing isobaric N,N-dimethyl leucine (DiLeu) tags and automated FDR-based large-scale data analysis by Byonic.
29687791	3	81	theme	FASP	849:852	arg1	method					855:860	the filter aided sample preparation (FASP) method	812:860	the filter aided sample preparation (FASP) method	812:860	In this study, an enhanced, dedicated, large-scale site-specific quantitative N-glycoproteomics workflow has been established, which includes improved specific extraction of membrane-bound glycoproteins using the filter aided sample preparation (FASP) method, enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment, site-specific N-glycopeptide characterization enabled by EThcD, relative quantitation utilizing isobaric N,N-dimethyl leucine (DiLeu) tags and automated FDR-based large-scale data analysis by Byonic.
29687791	1	82	gly	glycopeptides	254:266	arg2	glycopeptides					254:266	intact glycopeptides	247:266	intact glycopeptides	247:266	The system-wide site-specific analysis of intact glycopeptides is crucial for understanding the exact functional relevance of protein glycosylation.
29687791	3	83	theme	site-specific	654:666	arg1	workflow					699:706	an enhanced, dedicated, large-scale site-specific quantitative N-glycoproteomics workflow	618:706	an enhanced, dedicated, large-scale site-specific quantitative N-glycoproteomics workflow	618:706	In this study, an enhanced, dedicated, large-scale site-specific quantitative N-glycoproteomics workflow has been established, which includes improved specific extraction of membrane-bound glycoproteins using the filter aided sample preparation (FASP) method, enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment, site-specific N-glycopeptide characterization enabled by EThcD, relative quantitation utilizing isobaric N,N-dimethyl leucine (DiLeu) tags and automated FDR-based large-scale data analysis by Byonic.
29687791	6	84	theme	PKM2	1689:1692	arg1	cells					1703:1707	PKM2 knockout cells	1689:1707	PKM2 knockout cells	1689:1707	We further applied this approach to study the glycosylation alterations in PKM2 knockout cells vs. parental breast cancer cells and revealed altered N-glycoprotein/N-glycopeptide patterns and very different glycosylation microheterogeneity for different types of glycans.
29687791	1	85	theme	system-wide	209:219	arg1	crucial					271:277	crucial	271:277	crucial	271:277	The system-wide site-specific analysis of intact glycopeptides is crucial for understanding the exact functional relevance of protein glycosylation.
29687791	1	85	theme	system-wide	209:219	arg1	analysis					235:242	The system-wide site-specific analysis	205:242	The system-wide site-specific analysis of intact glycopeptides	205:266	The system-wide site-specific analysis of intact glycopeptides is crucial for understanding the exact functional relevance of protein glycosylation.
29687791	3	86	theme	large-scale	1180:1190	arg1	analysis					1197:1204	automated FDR-based large-scale data analysis	1160:1204	automated FDR-based large-scale data analysis	1160:1204	In this study, an enhanced, dedicated, large-scale site-specific quantitative N-glycoproteomics workflow has been established, which includes improved specific extraction of membrane-bound glycoproteins using the filter aided sample preparation (FASP) method, enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment, site-specific N-glycopeptide characterization enabled by EThcD, relative quantitation utilizing isobaric N,N-dimethyl leucine (DiLeu) tags and automated FDR-based large-scale data analysis by Byonic.
29687791	5	87	theme	glycan	1570:1575	arg1	compositions					1577:1588	88 glycan compositions	1567:1588	88 glycan compositions from 205 glycoproteins	1567:1611	When applying the developed workflow to site-specific N-glycoproteome study in PANC1 cells, we were able to identify 1067 intact N-glycopeptides, representing 311 glycosylation sites and 88 glycan compositions from 205 glycoproteins.
29687791	2	88	theme	dedicated	356:364	arg1	essential					509:517	essential	509:517	essential	509:517	A dedicated workflow with the capability to simultaneously characterize and quantify intact glycopeptides in a site-specific and high-throughput manner is essential to reveal specific glycosylation alteration patterns in complex biological systems.
29687791	2	88	theme	dedicated	356:364	arg1	workflow					366:373	A dedicated workflow	354:373	A dedicated workflow with the capability to simultaneously characterize and quantify intact glycopeptides in a site-specific and high-throughput manner	354:504	A dedicated workflow with the capability to simultaneously characterize and quantify intact glycopeptides in a site-specific and high-throughput manner is essential to reveal specific glycosylation alteration patterns in complex biological systems.
29687791	9	89	theme	pathway	2166:2172	arg1	analysis					2174:2181	KEGG pathway analysis	2161:2181	KEGG pathway analysis	2161:2181	KEGG pathway analysis revealed that the PI3K/Akt signaling pathway was highly enriched, which provided evidence to support the previous finding that PKM2 knockdown cancer cells rely on activation of Akt for their survival.
29687791	3	90	theme	enhanced	863:870	arg1	enrichment					872:881	enhanced enrichment	863:881	enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment	863:1014	In this study, an enhanced, dedicated, large-scale site-specific quantitative N-glycoproteomics workflow has been established, which includes improved specific extraction of membrane-bound glycoproteins using the filter aided sample preparation (FASP) method, enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment, site-specific N-glycopeptide characterization enabled by EThcD, relative quantitation utilizing isobaric N,N-dimethyl leucine (DiLeu) tags and automated FDR-based large-scale data analysis by Byonic.
29687791	3	90	theme	enhanced	863:870	arg1	method					855:860	the filter aided sample preparation (FASP) method	812:860	the filter aided sample preparation (FASP) method	812:860	In this study, an enhanced, dedicated, large-scale site-specific quantitative N-glycoproteomics workflow has been established, which includes improved specific extraction of membrane-bound glycoproteins using the filter aided sample preparation (FASP) method, enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment, site-specific N-glycopeptide characterization enabled by EThcD, relative quantitation utilizing isobaric N,N-dimethyl leucine (DiLeu) tags and automated FDR-based large-scale data analysis by Byonic.
29687791	0	91	theme	collision	173:181	arg1	EThcD					197:201	EThcD	197:201	EThcD	197:201	Site-specific characterization and quantitation of N-glycopeptides in PKM2 knockout breast cancer cells using DiLeu isobaric tags enabled by electron-transfer/higher-energy collision dissociation (EThcD).
29687791	0	91	theme	collision	173:181	arg1	dissociation					183:194	electron-transfer/higher-energy collision dissociation	141:194	electron-transfer/higher-energy collision dissociation (EThcD)	141:202	Site-specific characterization and quantitation of N-glycopeptides in PKM2 knockout breast cancer cells using DiLeu isobaric tags enabled by electron-transfer/higher-energy collision dissociation (EThcD).
29687791	1	92	theme	intact	247:252	arg1	glycopeptides					254:266	intact glycopeptides	247:266	intact glycopeptides	247:266	The system-wide site-specific analysis of intact glycopeptides is crucial for understanding the exact functional relevance of protein glycosylation.
29687791	3	93	theme	hydrophilic	919:929	arg1	chromatography					950:963	sequential hydrophilic interaction liquid chromatography	908:963	sequential hydrophilic interaction liquid chromatography (HILIC)	908:971	In this study, an enhanced, dedicated, large-scale site-specific quantitative N-glycoproteomics workflow has been established, which includes improved specific extraction of membrane-bound glycoproteins using the filter aided sample preparation (FASP) method, enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment, site-specific N-glycopeptide characterization enabled by EThcD, relative quantitation utilizing isobaric N,N-dimethyl leucine (DiLeu) tags and automated FDR-based large-scale data analysis by Byonic.
29687791	3	93	theme	hydrophilic	919:929	arg1	HILIC					966:970	HILIC	966:970	HILIC	966:970	In this study, an enhanced, dedicated, large-scale site-specific quantitative N-glycoproteomics workflow has been established, which includes improved specific extraction of membrane-bound glycoproteins using the filter aided sample preparation (FASP) method, enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment, site-specific N-glycopeptide characterization enabled by EThcD, relative quantitation utilizing isobaric N,N-dimethyl leucine (DiLeu) tags and automated FDR-based large-scale data analysis by Byonic.
29687791	3	94	theme	N-glycoproteomics	681:697	arg1	workflow					699:706	an enhanced, dedicated, large-scale site-specific quantitative N-glycoproteomics workflow	618:706	an enhanced, dedicated, large-scale site-specific quantitative N-glycoproteomics workflow	618:706	In this study, an enhanced, dedicated, large-scale site-specific quantitative N-glycoproteomics workflow has been established, which includes improved specific extraction of membrane-bound glycoproteins using the filter aided sample preparation (FASP) method, enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment, site-specific N-glycopeptide characterization enabled by EThcD, relative quantitation utilizing isobaric N,N-dimethyl leucine (DiLeu) tags and automated FDR-based large-scale data analysis by Byonic.
29687791	6	95	theme	altered	1755:1761	arg1	patterns					1793:1800	altered N-glycoprotein/N-glycopeptide patterns	1755:1800	altered N-glycoprotein/N-glycopeptide patterns	1755:1800	We further applied this approach to study the glycosylation alterations in PKM2 knockout cells vs. parental breast cancer cells and revealed altered N-glycoprotein/N-glycopeptide patterns and very different glycosylation microheterogeneity for different types of glycans.
29687791	6	96	gly	N-glycoprotein/N-glycopeptide	1763:1791	arg2	N-glycoprotein/N-glycopeptide					1763:1791	altered N-glycoprotein/N-glycopeptide patterns	1755:1800	altered N-glycoprotein/N-glycopeptide patterns	1755:1800	We further applied this approach to study the glycosylation alterations in PKM2 knockout cells vs. parental breast cancer cells and revealed altered N-glycoprotein/N-glycopeptide patterns and very different glycosylation microheterogeneity for different types of glycans.
29687791	9	97	theme	signaling	2210:2218	arg1	pathway					2220:2226	the PI3K/Akt signaling pathway	2197:2226	the PI3K/Akt signaling pathway	2197:2226	KEGG pathway analysis revealed that the PI3K/Akt signaling pathway was highly enriched, which provided evidence to support the previous finding that PKM2 knockdown cancer cells rely on activation of Akt for their survival.
29687791	5	98	theme	N-glycoproteome	1434:1448	arg1	study					1450:1454	site-specific N-glycoproteome study	1420:1454	site-specific N-glycoproteome study	1420:1454	When applying the developed workflow to site-specific N-glycoproteome study in PANC1 cells, we were able to identify 1067 intact N-glycopeptides, representing 311 glycosylation sites and 88 glycan compositions from 205 glycoproteins.
29687791	0	99	theme	breast	84:89	arg1	cells					98:102	PKM2 knockout breast cancer cells	70:102	PKM2 knockout breast cancer cells using DiLeu isobaric tags	70:128	Site-specific characterization and quantitation of N-glycopeptides in PKM2 knockout breast cancer cells using DiLeu isobaric tags enabled by electron-transfer/higher-energy collision dissociation (EThcD).
29687791	6	100	theme	cancer	1729:1734	arg1	cells					1736:1740	parental breast cancer cells	1713:1740	parental breast cancer cells	1713:1740	We further applied this approach to study the glycosylation alterations in PKM2 knockout cells vs. parental breast cancer cells and revealed altered N-glycoprotein/N-glycopeptide patterns and very different glycosylation microheterogeneity for different types of glycans.
29687791	0	101	from	quantitation	35:46	arg1	cells					98:102	PKM2 knockout breast cancer cells	70:102	PKM2 knockout breast cancer cells using DiLeu isobaric tags	70:128	Site-specific characterization and quantitation of N-glycopeptides in PKM2 knockout breast cancer cells using DiLeu isobaric tags enabled by electron-transfer/higher-energy collision dissociation (EThcD).
29687791	4	102	theme	MLA	1302:1304	arg1	enrichment					1306:1315	MLA enrichment	1302:1315	MLA enrichment with only 20% overlapping in enriching intact N-glycopeptides	1302:1377	For the first time, our study shows that HILIC complements to a very large extent to MLA enrichment with only 20% overlapping in enriching intact N-glycopeptides.
29687791	3	103	theme	liquid	943:948	arg1	chromatography					950:963	sequential hydrophilic interaction liquid chromatography	908:963	sequential hydrophilic interaction liquid chromatography (HILIC)	908:971	In this study, an enhanced, dedicated, large-scale site-specific quantitative N-glycoproteomics workflow has been established, which includes improved specific extraction of membrane-bound glycoproteins using the filter aided sample preparation (FASP) method, enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment, site-specific N-glycopeptide characterization enabled by EThcD, relative quantitation utilizing isobaric N,N-dimethyl leucine (DiLeu) tags and automated FDR-based large-scale data analysis by Byonic.
29687791	3	103	theme	liquid	943:948	arg1	HILIC					966:970	HILIC	966:970	HILIC	966:970	In this study, an enhanced, dedicated, large-scale site-specific quantitative N-glycoproteomics workflow has been established, which includes improved specific extraction of membrane-bound glycoproteins using the filter aided sample preparation (FASP) method, enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment, site-specific N-glycopeptide characterization enabled by EThcD, relative quantitation utilizing isobaric N,N-dimethyl leucine (DiLeu) tags and automated FDR-based large-scale data analysis by Byonic.
29687791	6	104	theme	parental	1713:1720	arg1	cells					1736:1740	parental breast cancer cells	1713:1740	parental breast cancer cells	1713:1740	We further applied this approach to study the glycosylation alterations in PKM2 knockout cells vs. parental breast cancer cells and revealed altered N-glycoprotein/N-glycopeptide patterns and very different glycosylation microheterogeneity for different types of glycans.
29687791	3	105	theme	N-glycopeptide	1031:1044	arg1	characterization					1046:1061	site-specific N-glycopeptide characterization	1017:1061	site-specific N-glycopeptide characterization	1017:1061	In this study, an enhanced, dedicated, large-scale site-specific quantitative N-glycoproteomics workflow has been established, which includes improved specific extraction of membrane-bound glycoproteins using the filter aided sample preparation (FASP) method, enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment, site-specific N-glycopeptide characterization enabled by EThcD, relative quantitation utilizing isobaric N,N-dimethyl leucine (DiLeu) tags and automated FDR-based large-scale data analysis by Byonic.
29687791	3	105	theme	N-glycopeptide	1031:1044	arg1	method					855:860	the filter aided sample preparation (FASP) method	812:860	the filter aided sample preparation (FASP) method	812:860	In this study, an enhanced, dedicated, large-scale site-specific quantitative N-glycoproteomics workflow has been established, which includes improved specific extraction of membrane-bound glycoproteins using the filter aided sample preparation (FASP) method, enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment, site-specific N-glycopeptide characterization enabled by EThcD, relative quantitation utilizing isobaric N,N-dimethyl leucine (DiLeu) tags and automated FDR-based large-scale data analysis by Byonic.
29687791	2	106	theme	complex	575:581	arg1	systems					594:600	complex biological systems	575:600	complex biological systems	575:600	A dedicated workflow with the capability to simultaneously characterize and quantify intact glycopeptides in a site-specific and high-throughput manner is essential to reveal specific glycosylation alteration patterns in complex biological systems.
29687791	0	107	from	characterization	14:29	arg1	cells					98:102	PKM2 knockout breast cancer cells	70:102	PKM2 knockout breast cancer cells using DiLeu isobaric tags	70:128	Site-specific characterization and quantitation of N-glycopeptides in PKM2 knockout breast cancer cells using DiLeu isobaric tags enabled by electron-transfer/higher-energy collision dissociation (EThcD).
29687791	3	108	gly	N-glycopeptide	1031:1044	arg2	N-glycopeptide					1031:1044	site-specific N-glycopeptide characterization	1017:1061	site-specific N-glycopeptide characterization	1017:1061	In this study, an enhanced, dedicated, large-scale site-specific quantitative N-glycoproteomics workflow has been established, which includes improved specific extraction of membrane-bound glycoproteins using the filter aided sample preparation (FASP) method, enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment, site-specific N-glycopeptide characterization enabled by EThcD, relative quantitation utilizing isobaric N,N-dimethyl leucine (DiLeu) tags and automated FDR-based large-scale data analysis by Byonic.
29687791	5	109	theme	developed	1398:1406	arg1	workflow					1408:1415	the developed workflow	1394:1415	the developed workflow to site-specific N-glycoproteome study in PANC1 cells	1394:1469	When applying the developed workflow to site-specific N-glycoproteome study in PANC1 cells, we were able to identify 1067 intact N-glycopeptides, representing 311 glycosylation sites and 88 glycan compositions from 205 glycoproteins.
29687791	3	110	theme	multi-lectin	977:988	arg1	enrichment					1005:1014	multi-lectin affinity (MLA) enrichment	977:1014	multi-lectin affinity (MLA) enrichment	977:1014	In this study, an enhanced, dedicated, large-scale site-specific quantitative N-glycoproteomics workflow has been established, which includes improved specific extraction of membrane-bound glycoproteins using the filter aided sample preparation (FASP) method, enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment, site-specific N-glycopeptide characterization enabled by EThcD, relative quantitation utilizing isobaric N,N-dimethyl leucine (DiLeu) tags and automated FDR-based large-scale data analysis by Byonic.
29687791	0	111	theme	DiLeu	110:114	arg1	tags					125:128	DiLeu isobaric tags	110:128	DiLeu isobaric tags	110:128	Site-specific characterization and quantitation of N-glycopeptides in PKM2 knockout breast cancer cells using DiLeu isobaric tags enabled by electron-transfer/higher-energy collision dissociation (EThcD).
29687791	10	112	theme	modulators	2445:2454	arg1	one					2409:2411	one	2409:2411	one	2409:2411	With glycosylation being one of the most important signaling modulators, our results provide additional evidence that signaling pathways are closely regulated by metabolism.
29687791	10	112	theme	modulators	2445:2454	arg1	modulators					2445:2454	the most important signaling modulators	2416:2454	the most important signaling modulators	2416:2454	With glycosylation being one of the most important signaling modulators, our results provide additional evidence that signaling pathways are closely regulated by metabolism.
29687791	8	113	theme	significant	2140:2150	arg1	changes					2152:2158	significant changes	2140:2158	significant changes	2140:2158	A total of 484 deglycosylated peptides were quantified, among which 81 deglycosylated peptides from 70 glycoproteins showed significant changes.
29687791	6	114	theme	glycosylation	1821:1833	arg1	microheterogeneity					1835:1852	very different glycosylation microheterogeneity	1806:1852	very different glycosylation microheterogeneity	1806:1852	We further applied this approach to study the glycosylation alterations in PKM2 knockout cells vs. parental breast cancer cells and revealed altered N-glycoprotein/N-glycopeptide patterns and very different glycosylation microheterogeneity for different types of glycans.
29687791	8	115	theme	deglycosylated	2031:2044	arg1	peptides					2046:2053	484 deglycosylated peptides	2027:2053	484 deglycosylated peptides	2027:2053	A total of 484 deglycosylated peptides were quantified, among which 81 deglycosylated peptides from 70 glycoproteins showed significant changes.
29687791	7	116	with	treatment	1972:1980	arg1	F					1994:1994	PNGase F	1987:1994	PNGase F	1987:1994	To obtain a more comprehensive map of glycoprotein alterations, N-glycopeptides after treatment with PNGase F were also analyzed.
29687791	1	117	theme	protein	331:337	arg1	glycosylation					339:351	protein glycosylation	331:351	protein glycosylation	331:351	The system-wide site-specific analysis of intact glycopeptides is crucial for understanding the exact functional relevance of protein glycosylation.
29687791	9	118	theme	previous	2288:2295	arg1	finding					2297:2303	the previous finding that PKM2 knockdown cancer cells rely on activation of Akt for their survival	2284:2381	the previous finding that PKM2 knockdown cancer cells rely on activation of Akt for their survival	2284:2381	KEGG pathway analysis revealed that the PI3K/Akt signaling pathway was highly enriched, which provided evidence to support the previous finding that PKM2 knockdown cancer cells rely on activation of Akt for their survival.
29687791	0	119	theme	N-glycopeptides	51:65	arg1	quantitation					35:46	quantitation	35:46	quantitation	35:46	Site-specific characterization and quantitation of N-glycopeptides in PKM2 knockout breast cancer cells using DiLeu isobaric tags enabled by electron-transfer/higher-energy collision dissociation (EThcD).
29687791	0	119	theme	N-glycopeptides	51:65	arg1	characterization					14:29	Site-specific characterization	0:29	Site-specific characterization	0:29	Site-specific characterization and quantitation of N-glycopeptides in PKM2 knockout breast cancer cells using DiLeu isobaric tags enabled by electron-transfer/higher-energy collision dissociation (EThcD).
29687791	3	120	theme	relative	1081:1088	arg1	quantitation					1090:1101	relative quantitation	1081:1101	relative quantitation utilizing isobaric N	1081:1122	In this study, an enhanced, dedicated, large-scale site-specific quantitative N-glycoproteomics workflow has been established, which includes improved specific extraction of membrane-bound glycoproteins using the filter aided sample preparation (FASP) method, enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment, site-specific N-glycopeptide characterization enabled by EThcD, relative quantitation utilizing isobaric N,N-dimethyl leucine (DiLeu) tags and automated FDR-based large-scale data analysis by Byonic.
29687791	3	121	theme	improved	745:752	arg1	extraction					763:772	improved specific extraction	745:772	improved specific extraction of membrane-bound glycoproteins using the filter aided sample preparation (FASP) method, enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment, site-specific N-glycopeptide characterization enabled by EThcD, relative quantitation utilizing isobaric N,N-dimethyl leucine (DiLeu) tags and automated FDR-based large-scale data analysis by Byonic	745:1214	In this study, an enhanced, dedicated, large-scale site-specific quantitative N-glycoproteomics workflow has been established, which includes improved specific extraction of membrane-bound glycoproteins using the filter aided sample preparation (FASP) method, enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment, site-specific N-glycopeptide characterization enabled by EThcD, relative quantitation utilizing isobaric N,N-dimethyl leucine (DiLeu) tags and automated FDR-based large-scale data analysis by Byonic.
29687791	0	122	theme	PKM2	70:73	arg1	cells					98:102	PKM2 knockout breast cancer cells	70:102	PKM2 knockout breast cancer cells using DiLeu isobaric tags	70:128	Site-specific characterization and quantitation of N-glycopeptides in PKM2 knockout breast cancer cells using DiLeu isobaric tags enabled by electron-transfer/higher-energy collision dissociation (EThcD).
29687791	3	123	theme	N-dimethyl	1124:1133	arg1	tags					1151:1154	N-dimethyl leucine (DiLeu) tags	1124:1154	N-dimethyl leucine (DiLeu) tags	1124:1154	In this study, an enhanced, dedicated, large-scale site-specific quantitative N-glycoproteomics workflow has been established, which includes improved specific extraction of membrane-bound glycoproteins using the filter aided sample preparation (FASP) method, enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment, site-specific N-glycopeptide characterization enabled by EThcD, relative quantitation utilizing isobaric N,N-dimethyl leucine (DiLeu) tags and automated FDR-based large-scale data analysis by Byonic.
29687791	2	124	theme	glycosylation	538:550	arg1	patterns					563:570	specific glycosylation alteration patterns	529:570	specific glycosylation alteration patterns in complex biological systems	529:600	A dedicated workflow with the capability to simultaneously characterize and quantify intact glycopeptides in a site-specific and high-throughput manner is essential to reveal specific glycosylation alteration patterns in complex biological systems.
29687791	9	125	theme	knockdown	2315:2323	arg1	cells					2332:2336	PKM2 knockdown cancer cells	2310:2336	PKM2 knockdown cancer cells	2310:2336	KEGG pathway analysis revealed that the PI3K/Akt signaling pathway was highly enriched, which provided evidence to support the previous finding that PKM2 knockdown cancer cells rely on activation of Akt for their survival.
29687791	7	126	gly	N-glycopeptides	1950:1964	arg2	N-glycopeptides					1950:1964	N-glycopeptides	1950:1964	N-glycopeptides after treatment with PNGase F	1950:1994	To obtain a more comprehensive map of glycoprotein alterations, N-glycopeptides after treatment with PNGase F were also analyzed.
29687791	4	127	theme	first	1225:1229	arg1	time					1231:1234	the first time	1221:1234	the first time	1221:1234	For the first time, our study shows that HILIC complements to a very large extent to MLA enrichment with only 20% overlapping in enriching intact N-glycopeptides.
29687791	3	128	theme	membrane-bound	777:790	arg1	glycoproteins					792:804	membrane-bound glycoproteins	777:804	membrane-bound glycoproteins using the filter aided sample preparation (FASP) method, enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment, site-specific N-glycopeptide characterization enabled by EThcD, relative quantitation utilizing isobaric N,N-dimethyl leucine (DiLeu) tags and automated FDR-based large-scale data analysis by Byonic	777:1214	In this study, an enhanced, dedicated, large-scale site-specific quantitative N-glycoproteomics workflow has been established, which includes improved specific extraction of membrane-bound glycoproteins using the filter aided sample preparation (FASP) method, enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment, site-specific N-glycopeptide characterization enabled by EThcD, relative quantitation utilizing isobaric N,N-dimethyl leucine (DiLeu) tags and automated FDR-based large-scale data analysis by Byonic.
29687791	4	129	gly	N-glycopeptides	1363:1377	arg2	N-glycopeptides					1363:1377	intact N-glycopeptides	1356:1377	intact N-glycopeptides	1356:1377	For the first time, our study shows that HILIC complements to a very large extent to MLA enrichment with only 20% overlapping in enriching intact N-glycopeptides.
29687791	3	130	gly	glycoproteins	792:804	arg1	glycoproteins					792:804	membrane-bound glycoproteins	777:804	membrane-bound glycoproteins using the filter aided sample preparation (FASP) method, enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment, site-specific N-glycopeptide characterization enabled by EThcD, relative quantitation utilizing isobaric N,N-dimethyl leucine (DiLeu) tags and automated FDR-based large-scale data analysis by Byonic	777:1214	In this study, an enhanced, dedicated, large-scale site-specific quantitative N-glycoproteomics workflow has been established, which includes improved specific extraction of membrane-bound glycoproteins using the filter aided sample preparation (FASP) method, enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment, site-specific N-glycopeptide characterization enabled by EThcD, relative quantitation utilizing isobaric N,N-dimethyl leucine (DiLeu) tags and automated FDR-based large-scale data analysis by Byonic.
29687791	3	131	theme	sample	829:834	arg1	enrichment					872:881	enhanced enrichment	863:881	enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment	863:1014	In this study, an enhanced, dedicated, large-scale site-specific quantitative N-glycoproteomics workflow has been established, which includes improved specific extraction of membrane-bound glycoproteins using the filter aided sample preparation (FASP) method, enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment, site-specific N-glycopeptide characterization enabled by EThcD, relative quantitation utilizing isobaric N,N-dimethyl leucine (DiLeu) tags and automated FDR-based large-scale data analysis by Byonic.
29687791	3	131	theme	sample	829:834	arg1	characterization					1046:1061	site-specific N-glycopeptide characterization	1017:1061	site-specific N-glycopeptide characterization	1017:1061	In this study, an enhanced, dedicated, large-scale site-specific quantitative N-glycoproteomics workflow has been established, which includes improved specific extraction of membrane-bound glycoproteins using the filter aided sample preparation (FASP) method, enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment, site-specific N-glycopeptide characterization enabled by EThcD, relative quantitation utilizing isobaric N,N-dimethyl leucine (DiLeu) tags and automated FDR-based large-scale data analysis by Byonic.
29687791	3	131	theme	sample	829:834	arg1	method					855:860	the filter aided sample preparation (FASP) method	812:860	the filter aided sample preparation (FASP) method	812:860	In this study, an enhanced, dedicated, large-scale site-specific quantitative N-glycoproteomics workflow has been established, which includes improved specific extraction of membrane-bound glycoproteins using the filter aided sample preparation (FASP) method, enhanced enrichment of N-glycopeptides using sequential hydrophilic interaction liquid chromatography (HILIC) and multi-lectin affinity (MLA) enrichment, site-specific N-glycopeptide characterization enabled by EThcD, relative quantitation utilizing isobaric N,N-dimethyl leucine (DiLeu) tags and automated FDR-based large-scale data analysis by Byonic.
29687791	5	132	theme	intact	1502:1507	arg1	N-glycopeptides					1509:1523	1067 intact N-glycopeptides	1497:1523	1067 intact N-glycopeptides	1497:1523	When applying the developed workflow to site-specific N-glycoproteome study in PANC1 cells, we were able to identify 1067 intact N-glycopeptides, representing 311 glycosylation sites and 88 glycan compositions from 205 glycoproteins.
29533934	1	0	theme	cytokine	146:153	arg1	IL-11					171:175	IL-11	171:175	IL-11	171:175	BACKGROUND/AIMS: The cytokine interleukin-11 (IL-11) has important pro- and anti-inflammatory functions.
29533934	1	0	theme	cytokine	146:153	arg1	interleukin-11					155:168	The cytokine interleukin-11	142:168	The cytokine interleukin-11 (IL-11)	142:176	BACKGROUND/AIMS: The cytokine interleukin-11 (IL-11) has important pro- and anti-inflammatory functions.
29533934	9	1	theme	IL-11RN127Q/N194Q	1452:1468	arg1	mutant					1470:1475	the IL-11RN127Q/N194Q mutant	1448:1475	the IL-11RN127Q/N194Q mutant	1448:1475	Furthermore, the IL-11RN127Q/N194Q mutant shows no inducible shedding by ADAM10, but is rather constitutively released into the supernatant.
29533934	7	2	theme	sequons	1311:1317	arg1	role					1286:1289	a differential role	1271:1289	a differential role of the two N-glycan sequons for IL-11R maturation	1271:1339	Accordingly, IL-11R mutants lacking N194 are retained within the ER, whereas the N127 mutant is transported through the Golgi complex to the cell surface, uncovering a differential role of the two N-glycan sequons for IL-11R maturation.
29533934	6	3	theme	IL-11R	1063:1068	arg1	appearance					1070:1079	IL-11R appearance	1063:1079	IL-11R appearance	1063:1079	While mutation of N127Q only slightly affects cell surface expression of the IL-11R, mutation of N194Q broadly prevents IL-11R appearance at the plasma membrane.
29533934	4	4	theme	confocal	773:780	arg1	microscopy					782:791	confocal microscopy	773:791	confocal microscopy	773:791	METHODS: We generated different IL-11R mutants via site-directed mutagenesis and analyzed them in different cell lines via Western blot, flow cytometry, confocal microscopy and proliferation assays.
29533934	2	5	theme	IL-11	283:287	arg1	IL-11R					299:304	IL-11R	299:304	IL-11R	299:304	It activates its target cells through binding to the IL-11 receptor (IL-11R), and the IL-11/IL-11R complex recruits a homodimer of glycoprotein 130 (gp130).
29533934	2	5	theme	IL-11	283:287	arg1	receptor					289:296	IL-11 receptor	283:296	the IL-11 receptor (IL-11R)	279:305	It activates its target cells through binding to the IL-11 receptor (IL-11R), and the IL-11/IL-11R complex recruits a homodimer of glycoprotein 130 (gp130).
29533934	8	6	theme	one	1382:1384	arg1	devoid					1372:1377	devoid	1372:1377	devoid	1372:1377	Interestingly, IL-11R mutants devoid of one or both N-glycans are still biologically active.
29533934	6	7	theme	N127Q	961:965	arg1	mutation					949:956	mutation	949:956	mutation of N127Q	949:965	While mutation of N127Q only slightly affects cell surface expression of the IL-11R, mutation of N194Q broadly prevents IL-11R appearance at the plasma membrane.
29533934	7	8	theme	Golgi	1225:1229	arg1	complex					1231:1237	the Golgi complex	1221:1237	the Golgi complex	1221:1237	Accordingly, IL-11R mutants lacking N194 are retained within the ER, whereas the N127 mutant is transported through the Golgi complex to the cell surface, uncovering a differential role of the two N-glycan sequons for IL-11R maturation.
29533934	10	9	theme	N-linked	1726:1733	arg1	glycosylation					1735:1747	N-linked glycosylation	1726:1747	N-linked glycosylation	1726:1747	CONCLUSION: Our results show that the two N-glycosylation sites differentially influence stability and proteolytic processing of the IL-11R, but that N-linked glycosylation is not a prerequisite for IL-11 signaling.
29533934	10	9	theme	N-linked	1726:1733	arg1	prerequisite					1758:1769	a prerequisite	1756:1769	a prerequisite for IL-11 signaling	1756:1789	CONCLUSION: Our results show that the two N-glycosylation sites differentially influence stability and proteolytic processing of the IL-11R, but that N-linked glycosylation is not a prerequisite for IL-11 signaling.
29533934	3	10	theme	side	496:499	arg1	chain					501:505	the side chain	492:505	the side chain of asparagine residues	492:528	N-linked glycosylation, a post-translational modification where complex oligosaccharides are attached to the side chain of asparagine residues, is often important for stability, folding and biological function of cytokine receptors.
29533934	10	11	theme	proteolytic	1679:1689	arg1	processing					1691:1700	proteolytic processing	1679:1700	proteolytic processing	1679:1700	CONCLUSION: Our results show that the two N-glycosylation sites differentially influence stability and proteolytic processing of the IL-11R, but that N-linked glycosylation is not a prerequisite for IL-11 signaling.
29533934	5	12	theme	D2	901:902	arg1	domain					904:909	the D2 domain	897:909	the D2 domain of the IL-11R	897:923	RESULTS: In this study, we identified two functional N-glycosylation sites in the D2 domain of the IL-11R at N127 and N194.
29533934	5	12	theme	D2	901:902	arg1	IL-11R					918:923	the IL-11R	914:923	the IL-11R	914:923	RESULTS: In this study, we identified two functional N-glycosylation sites in the D2 domain of the IL-11R at N127 and N194.
29533934	6	13	theme	IL-11R	1020:1025	arg1	expression					1002:1011	cell surface expression	989:1011	cell surface expression of the IL-11R	989:1025	While mutation of N127Q only slightly affects cell surface expression of the IL-11R, mutation of N194Q broadly prevents IL-11R appearance at the plasma membrane.
29533934	3	14	theme	residues	521:528	arg1	chain					501:505	the side chain	492:505	the side chain of asparagine residues	492:528	N-linked glycosylation, a post-translational modification where complex oligosaccharides are attached to the side chain of asparagine residues, is often important for stability, folding and biological function of cytokine receptors.
29533934	4	15	theme	IL-11R	652:657	arg1	mutants					659:665	different IL-11R mutants	642:665	different IL-11R mutants	642:665	METHODS: We generated different IL-11R mutants via site-directed mutagenesis and analyzed them in different cell lines via Western blot, flow cytometry, confocal microscopy and proliferation assays.
29533934	6	16	theme	surface	994:1000	arg1	expression					1002:1011	cell surface expression	989:1011	cell surface expression of the IL-11R	989:1025	While mutation of N127Q only slightly affects cell surface expression of the IL-11R, mutation of N194Q broadly prevents IL-11R appearance at the plasma membrane.
29533934	3	17	theme	post-translational	413:430	arg1	glycosylation					396:408	N-linked glycosylation	387:408	N-linked glycosylation	387:408	N-linked glycosylation, a post-translational modification where complex oligosaccharides are attached to the side chain of asparagine residues, is often important for stability, folding and biological function of cytokine receptors.
29533934	3	17	theme	post-translational	413:430	arg1	modification					432:443	a post-translational modification	411:443	a post-translational modification where complex oligosaccharides are attached to the side chain of asparagine residues	411:528	N-linked glycosylation, a post-translational modification where complex oligosaccharides are attached to the side chain of asparagine residues, is often important for stability, folding and biological function of cytokine receptors.
29533934	0	18	theme	N-Linked	4:11	arg1	Glycans					13:19	Two N-Linked Glycans	0:19	Two N-Linked Glycans	0:19	Two N-Linked Glycans Differentially Control Maturation, Trafficking and Proteolysis, but not Activity of the IL-11 Receptor.
29533934	8	19	theme	devoid	1372:1377	arg1	mutants					1364:1370	IL-11R mutants	1357:1370	IL-11R mutants devoid of one or both N-glycans	1357:1402	Interestingly, IL-11R mutants devoid of one or both N-glycans are still biologically active.
29533934	9	20	theme	inducible	1486:1494	arg1	shedding					1496:1503	no inducible shedding	1483:1503	no inducible shedding by ADAM10	1483:1513	Furthermore, the IL-11RN127Q/N194Q mutant shows no inducible shedding by ADAM10, but is rather constitutively released into the supernatant.
29533934	5	21	theme	IL-11R	918:923	arg1	domain					904:909	the D2 domain	897:909	the D2 domain of the IL-11R	897:923	RESULTS: In this study, we identified two functional N-glycosylation sites in the D2 domain of the IL-11R at N127 and N194.
29533934	5	21	theme	IL-11R	918:923	arg1	IL-11R					918:923	the IL-11R	914:923	the IL-11R	914:923	RESULTS: In this study, we identified two functional N-glycosylation sites in the D2 domain of the IL-11R at N127 and N194.
29533934	2	22	theme	glycoprotein	361:372	arg1	homodimer					348:356	a homodimer	346:356	a homodimer of glycoprotein 130 (gp130)	346:384	It activates its target cells through binding to the IL-11 receptor (IL-11R), and the IL-11/IL-11R complex recruits a homodimer of glycoprotein 130 (gp130).
29533934	2	23	gly	glycoprotein	361:372	arg1	glycoprotein					361:372	glycoprotein 130	361:376	glycoprotein 130 (gp130)	361:384	It activates its target cells through binding to the IL-11 receptor (IL-11R), and the IL-11/IL-11R complex recruits a homodimer of glycoprotein 130 (gp130).
29533934	2	23	gly	glycoprotein	361:372	arg1	gp130					379:383	gp130	379:383	gp130	379:383	It activates its target cells through binding to the IL-11 receptor (IL-11R), and the IL-11/IL-11R complex recruits a homodimer of glycoprotein 130 (gp130).
29533934	5	24	theme	functional	861:870	arg1	sites					888:892	two functional N-glycosylation sites	857:892	two functional N-glycosylation sites	857:892	RESULTS: In this study, we identified two functional N-glycosylation sites in the D2 domain of the IL-11R at N127 and N194.
29533934	4	25	theme	proliferation	797:809	arg1	assays					811:816	proliferation assays	797:816	proliferation assays	797:816	METHODS: We generated different IL-11R mutants via site-directed mutagenesis and analyzed them in different cell lines via Western blot, flow cytometry, confocal microscopy and proliferation assays.
29533934	0	26	theme	Receptor	115:122	arg1	Activity					93:100	Activity	93:100	Activity of the IL-11 Receptor	93:122	Two N-Linked Glycans Differentially Control Maturation, Trafficking and Proteolysis, but not Activity of the IL-11 Receptor.
29533934	7	27	theme	IL-11R	1323:1328	arg1	maturation					1330:1339	IL-11R maturation	1323:1339	IL-11R maturation	1323:1339	Accordingly, IL-11R mutants lacking N194 are retained within the ER, whereas the N127 mutant is transported through the Golgi complex to the cell surface, uncovering a differential role of the two N-glycan sequons for IL-11R maturation.
29533934	6	28	theme	N194Q	1040:1044	arg1	mutation					1028:1035	mutation	1028:1035	mutation of N194Q	1028:1044	While mutation of N127Q only slightly affects cell surface expression of the IL-11R, mutation of N194Q broadly prevents IL-11R appearance at the plasma membrane.
29533934	3	29	theme	complex	451:457	arg1	oligosaccharides					459:474	complex oligosaccharides	451:474	complex oligosaccharides	451:474	N-linked glycosylation, a post-translational modification where complex oligosaccharides are attached to the side chain of asparagine residues, is often important for stability, folding and biological function of cytokine receptors.
29533934	0	30	theme	IL-11	109:113	arg1	Receptor					115:122	the IL-11 Receptor	105:122	the IL-11 Receptor	105:122	Two N-Linked Glycans Differentially Control Maturation, Trafficking and Proteolysis, but not Activity of the IL-11 Receptor.
29533934	1	31	contain	has	178:180	arg2	functions					219:227	important pro- and anti-inflammatory functions	182:227	important pro- and anti-inflammatory functions	182:227	BACKGROUND/AIMS: The cytokine interleukin-11 (IL-11) has important pro- and anti-inflammatory functions.
29533934	1	31	contain	has	178:180	arg1	IL-11					171:175	IL-11	171:175	IL-11	171:175	BACKGROUND/AIMS: The cytokine interleukin-11 (IL-11) has important pro- and anti-inflammatory functions.
29533934	1	31	contain	has	178:180	arg1	interleukin-11					155:168	The cytokine interleukin-11	142:168	The cytokine interleukin-11 (IL-11)	142:176	BACKGROUND/AIMS: The cytokine interleukin-11 (IL-11) has important pro- and anti-inflammatory functions.
29533934	4	32	theme	Western	743:749	arg1	blot					751:754	Western blot	743:754	Western blot	743:754	METHODS: We generated different IL-11R mutants via site-directed mutagenesis and analyzed them in different cell lines via Western blot, flow cytometry, confocal microscopy and proliferation assays.
29533934	1	33	theme	pro-	192:195	arg1	functions					219:227	important pro- and anti-inflammatory functions	182:227	important pro- and anti-inflammatory functions	182:227	BACKGROUND/AIMS: The cytokine interleukin-11 (IL-11) has important pro- and anti-inflammatory functions.
29533934	7	34	theme	IL-11R	1118:1123	arg1	mutants					1125:1131	IL-11R mutants	1118:1131	IL-11R mutants lacking N194	1118:1144	Accordingly, IL-11R mutants lacking N194 are retained within the ER, whereas the N127 mutant is transported through the Golgi complex to the cell surface, uncovering a differential role of the two N-glycan sequons for IL-11R maturation.
29533934	10	35	link	N-linked	1726:1733	arg1	glycosylation					1735:1747	N-linked glycosylation	1726:1747	N-linked glycosylation	1726:1747	CONCLUSION: Our results show that the two N-glycosylation sites differentially influence stability and proteolytic processing of the IL-11R, but that N-linked glycosylation is not a prerequisite for IL-11 signaling.
29533934	10	35	link	N-linked	1726:1733	arg1	prerequisite					1758:1769	a prerequisite	1756:1769	a prerequisite for IL-11 signaling	1756:1789	CONCLUSION: Our results show that the two N-glycosylation sites differentially influence stability and proteolytic processing of the IL-11R, but that N-linked glycosylation is not a prerequisite for IL-11 signaling.
29533934	8	36	theme	both	1389:1392	arg1	devoid					1372:1377	devoid	1372:1377	devoid	1372:1377	Interestingly, IL-11R mutants devoid of one or both N-glycans are still biologically active.
29533934	3	37	theme	biological	577:586	arg1	function					588:595	biological function	577:595	biological function	577:595	N-linked glycosylation, a post-translational modification where complex oligosaccharides are attached to the side chain of asparagine residues, is often important for stability, folding and biological function of cytokine receptors.
29533934	7	38	theme	differential	1273:1284	arg1	role					1286:1289	a differential role	1271:1289	a differential role of the two N-glycan sequons for IL-11R maturation	1271:1339	Accordingly, IL-11R mutants lacking N194 are retained within the ER, whereas the N127 mutant is transported through the Golgi complex to the cell surface, uncovering a differential role of the two N-glycan sequons for IL-11R maturation.
29533934	7	39	theme	cell	1246:1249	arg1	surface					1251:1257	the cell surface	1242:1257	the cell surface	1242:1257	Accordingly, IL-11R mutants lacking N194 are retained within the ER, whereas the N127 mutant is transported through the Golgi complex to the cell surface, uncovering a differential role of the two N-glycan sequons for IL-11R maturation.
29533934	3	40	theme	N-linked	387:394	arg1	glycosylation					396:408	N-linked glycosylation	387:408	N-linked glycosylation	387:408	N-linked glycosylation, a post-translational modification where complex oligosaccharides are attached to the side chain of asparagine residues, is often important for stability, folding and biological function of cytokine receptors.
29533934	3	40	theme	N-linked	387:394	arg1	modification					432:443	a post-translational modification	411:443	a post-translational modification where complex oligosaccharides are attached to the side chain of asparagine residues	411:528	N-linked glycosylation, a post-translational modification where complex oligosaccharides are attached to the side chain of asparagine residues, is often important for stability, folding and biological function of cytokine receptors.
29533934	10	41	gly	N-glycosylation	1618:1632	arg2	sites					1634:1638	the two N-glycosylation sites	1610:1638	the two N-glycosylation sites	1610:1638	CONCLUSION: Our results show that the two N-glycosylation sites differentially influence stability and proteolytic processing of the IL-11R, but that N-linked glycosylation is not a prerequisite for IL-11 signaling.
29533934	10	41	gly	N-glycosylation	1618:1632	arg2	two					1614:1616	two	1614:1616	two	1614:1616	CONCLUSION: Our results show that the two N-glycosylation sites differentially influence stability and proteolytic processing of the IL-11R, but that N-linked glycosylation is not a prerequisite for IL-11 signaling.
29533934	1	42	theme	anti-inflammatory	201:217	arg1	functions					219:227	important pro- and anti-inflammatory functions	182:227	important pro- and anti-inflammatory functions	182:227	BACKGROUND/AIMS: The cytokine interleukin-11 (IL-11) has important pro- and anti-inflammatory functions.
29533934	10	43	theme	IL-11	1775:1779	arg1	signaling					1781:1789	IL-11 signaling	1775:1789	IL-11 signaling	1775:1789	CONCLUSION: Our results show that the two N-glycosylation sites differentially influence stability and proteolytic processing of the IL-11R, but that N-linked glycosylation is not a prerequisite for IL-11 signaling.
29533934	2	44	theme	IL-11/IL-11R	316:327	arg1	complex					329:335	the IL-11/IL-11R complex	312:335	the IL-11/IL-11R complex	312:335	It activates its target cells through binding to the IL-11 receptor (IL-11R), and the IL-11/IL-11R complex recruits a homodimer of glycoprotein 130 (gp130).
29533934	10	45	theme	IL-11R	1709:1714	arg1	stability					1665:1673	stability	1665:1673	stability	1665:1673	CONCLUSION: Our results show that the two N-glycosylation sites differentially influence stability and proteolytic processing of the IL-11R, but that N-linked glycosylation is not a prerequisite for IL-11 signaling.
29533934	10	45	theme	IL-11R	1709:1714	arg1	processing					1691:1700	proteolytic processing	1679:1700	proteolytic processing	1679:1700	CONCLUSION: Our results show that the two N-glycosylation sites differentially influence stability and proteolytic processing of the IL-11R, but that N-linked glycosylation is not a prerequisite for IL-11 signaling.
29533934	5	46	gly	N-glycosylation	872:886	arg2	two					857:859	two	857:859	two	857:859	RESULTS: In this study, we identified two functional N-glycosylation sites in the D2 domain of the IL-11R at N127 and N194.
29533934	5	46	gly	N-glycosylation	872:886	arg2	sites					888:892	two functional N-glycosylation sites	857:892	two functional N-glycosylation sites	857:892	RESULTS: In this study, we identified two functional N-glycosylation sites in the D2 domain of the IL-11R at N127 and N194.
29533934	3	47	link	N-linked	387:394	arg1	glycosylation					396:408	N-linked glycosylation	387:408	N-linked glycosylation	387:408	N-linked glycosylation, a post-translational modification where complex oligosaccharides are attached to the side chain of asparagine residues, is often important for stability, folding and biological function of cytokine receptors.
29533934	3	47	link	N-linked	387:394	arg1	modification					432:443	a post-translational modification	411:443	a post-translational modification where complex oligosaccharides are attached to the side chain of asparagine residues	411:528	N-linked glycosylation, a post-translational modification where complex oligosaccharides are attached to the side chain of asparagine residues, is often important for stability, folding and biological function of cytokine receptors.
29533934	8	48	dep	one	1382:1384	arg1	N-glycans					1394:1402	N-glycans	1394:1402	N-glycans	1394:1402	Interestingly, IL-11R mutants devoid of one or both N-glycans are still biologically active.
29533934	3	49	gly	glycosylation	396:408	arg1	receptors					609:617	cytokine receptors	600:617	cytokine receptors	600:617	N-linked glycosylation, a post-translational modification where complex oligosaccharides are attached to the side chain of asparagine residues, is often important for stability, folding and biological function of cytokine receptors.
29533934	7	50	theme	N-glycan	1302:1309	arg1	sequons					1311:1317	the two N-glycan sequons	1294:1317	the two N-glycan sequons	1294:1317	Accordingly, IL-11R mutants lacking N194 are retained within the ER, whereas the N127 mutant is transported through the Golgi complex to the cell surface, uncovering a differential role of the two N-glycan sequons for IL-11R maturation.
29533934	3	51	theme	receptors	609:617	arg1	folding					565:571	folding	565:571	folding	565:571	N-linked glycosylation, a post-translational modification where complex oligosaccharides are attached to the side chain of asparagine residues, is often important for stability, folding and biological function of cytokine receptors.
29533934	3	51	theme	receptors	609:617	arg1	function					588:595	biological function	577:595	biological function	577:595	N-linked glycosylation, a post-translational modification where complex oligosaccharides are attached to the side chain of asparagine residues, is often important for stability, folding and biological function of cytokine receptors.
29533934	3	51	theme	receptors	609:617	arg1	stability					554:562	stability	554:562	stability	554:562	N-linked glycosylation, a post-translational modification where complex oligosaccharides are attached to the side chain of asparagine residues, is often important for stability, folding and biological function of cytokine receptors.
29533934	4	52	theme	site-directed	671:683	arg1	mutagenesis					685:695	site-directed mutagenesis	671:695	site-directed mutagenesis	671:695	METHODS: We generated different IL-11R mutants via site-directed mutagenesis and analyzed them in different cell lines via Western blot, flow cytometry, confocal microscopy and proliferation assays.
29533934	4	53	theme	cell	728:731	arg1	lines					733:737	different cell lines	718:737	different cell lines	718:737	METHODS: We generated different IL-11R mutants via site-directed mutagenesis and analyzed them in different cell lines via Western blot, flow cytometry, confocal microscopy and proliferation assays.
29533934	4	54	theme	flow	757:760	arg1	cytometry					762:770	flow cytometry	757:770	flow cytometry	757:770	METHODS: We generated different IL-11R mutants via site-directed mutagenesis and analyzed them in different cell lines via Western blot, flow cytometry, confocal microscopy and proliferation assays.
29533934	4	55	theme	different	718:726	arg1	lines					733:737	different cell lines	718:737	different cell lines	718:737	METHODS: We generated different IL-11R mutants via site-directed mutagenesis and analyzed them in different cell lines via Western blot, flow cytometry, confocal microscopy and proliferation assays.
29533934	6	56	theme	plasma	1088:1093	arg1	membrane					1095:1102	the plasma membrane	1084:1102	the plasma membrane	1084:1102	While mutation of N127Q only slightly affects cell surface expression of the IL-11R, mutation of N194Q broadly prevents IL-11R appearance at the plasma membrane.
29533934	7	57	theme	N127	1186:1189	arg1	mutant					1191:1196	the N127 mutant	1182:1196	the N127 mutant	1182:1196	Accordingly, IL-11R mutants lacking N194 are retained within the ER, whereas the N127 mutant is transported through the Golgi complex to the cell surface, uncovering a differential role of the two N-glycan sequons for IL-11R maturation.
29533934	10	58	theme	N-glycosylation	1618:1632	arg1	sites					1634:1638	the two N-glycosylation sites	1610:1638	the two N-glycosylation sites	1610:1638	CONCLUSION: Our results show that the two N-glycosylation sites differentially influence stability and proteolytic processing of the IL-11R, but that N-linked glycosylation is not a prerequisite for IL-11 signaling.
29533934	2	59	theme	target	247:252	arg1	cells					254:258	its target cells	243:258	its target cells	243:258	It activates its target cells through binding to the IL-11 receptor (IL-11R), and the IL-11/IL-11R complex recruits a homodimer of glycoprotein 130 (gp130).
29533934	1	60	theme	important	182:190	arg1	functions					219:227	important pro- and anti-inflammatory functions	182:227	important pro- and anti-inflammatory functions	182:227	BACKGROUND/AIMS: The cytokine interleukin-11 (IL-11) has important pro- and anti-inflammatory functions.
29533934	5	61	theme	N-glycosylation	872:886	arg1	sites					888:892	two functional N-glycosylation sites	857:892	two functional N-glycosylation sites	857:892	RESULTS: In this study, we identified two functional N-glycosylation sites in the D2 domain of the IL-11R at N127 and N194.
29533934	4	62	theme	different	642:650	arg1	mutants					659:665	different IL-11R mutants	642:665	different IL-11R mutants	642:665	METHODS: We generated different IL-11R mutants via site-directed mutagenesis and analyzed them in different cell lines via Western blot, flow cytometry, confocal microscopy and proliferation assays.
29533934	6	63	theme	cell	989:992	arg1	expression					1002:1011	cell surface expression	989:1011	cell surface expression of the IL-11R	989:1025	While mutation of N127Q only slightly affects cell surface expression of the IL-11R, mutation of N194Q broadly prevents IL-11R appearance at the plasma membrane.
29533934	3	64	theme	cytokine	600:607	arg1	receptors					609:617	cytokine receptors	600:617	cytokine receptors	600:617	N-linked glycosylation, a post-translational modification where complex oligosaccharides are attached to the side chain of asparagine residues, is often important for stability, folding and biological function of cytokine receptors.
29533934	3	65	attach	attached	480:487	arg2	oligosaccharides					459:474	complex oligosaccharides	451:474	complex oligosaccharides	451:474	N-linked glycosylation, a post-translational modification where complex oligosaccharides are attached to the side chain of asparagine residues, is often important for stability, folding and biological function of cytokine receptors.
29533934	3	65	attach	attached	480:487	arg1	chain					501:505	the side chain	492:505	the side chain of asparagine residues	492:528	N-linked glycosylation, a post-translational modification where complex oligosaccharides are attached to the side chain of asparagine residues, is often important for stability, folding and biological function of cytokine receptors.
29533934	3	66	theme	asparagine	510:519	arg1	residues					521:528	asparagine residues	510:528	asparagine residues	510:528	N-linked glycosylation, a post-translational modification where complex oligosaccharides are attached to the side chain of asparagine residues, is often important for stability, folding and biological function of cytokine receptors.
29533934	8	67	theme	IL-11R	1357:1362	arg1	mutants					1364:1370	IL-11R mutants	1357:1370	IL-11R mutants devoid of one or both N-glycans	1357:1402	Interestingly, IL-11R mutants devoid of one or both N-glycans are still biologically active.
30056138	6	0	theme	suspension	896:905	arg1	cells					907:911	CHO suspension cells	892:911	CHO suspension cells	892:911	The expression of eelLHβ/α (wild-type) in CHO suspension cells was increased by approximately 2-fold higher than that of attached CHO cells.
30056138	12	1	theme	cAMP	1703:1706	arg1	response					1708:1715	the cAMP response	1699:1715	the cAMP response	1699:1715	The EC50 value of the cAMP response at Asn79 of the α-subunit was increased by 5-fold, whereas the maximum response was dramatically reduced to 17.8% of wild-type levels.
30056138	1	2	from	position	307:314	arg1	α-subunit					293:301	the α-subunit	289:301	the α-subunit	289:301	Eel luteinizing hormone (eelLH) is composed of a common α-subunit and hormone specific β-subunit, both of which contain asparagine-linked carbohydrate residues, located at positions 56 and 79 on the α-subunit and position 10 on the β-subunit.
30056138	1	2	from	position	307:314	arg1	β-subunit					326:334	the β-subunit	322:334	the β-subunit	322:334	Eel luteinizing hormone (eelLH) is composed of a common α-subunit and hormone specific β-subunit, both of which contain asparagine-linked carbohydrate residues, located at positions 56 and 79 on the α-subunit and position 10 on the β-subunit.
30056138	1	2	from	position	307:314	arg1	located					255:261	located	255:261	located	255:261	Eel luteinizing hormone (eelLH) is composed of a common α-subunit and hormone specific β-subunit, both of which contain asparagine-linked carbohydrate residues, located at positions 56 and 79 on the α-subunit and position 10 on the β-subunit.
30056138	1	3	theme	located	255:261	arg1	residues					245:252	asparagine-linked carbohydrate residues	214:252	asparagine-linked carbohydrate residues	214:252	Eel luteinizing hormone (eelLH) is composed of a common α-subunit and hormone specific β-subunit, both of which contain asparagine-linked carbohydrate residues, located at positions 56 and 79 on the α-subunit and position 10 on the β-subunit.
30056138	3	4	theme	terminal	522:529	arg1	region					531:536	the carboxyl terminal region	509:536	the carboxyl terminal region of the eelLH β-subunit	509:559	Thus, we characterized the biologically active single chains by fusing the α-subunit to the carboxyl terminal region of the eelLH β-subunit.
30056138	4	5	from	function	659:666	arg1	secretion					705:713	secretion	705:713	secretion	705:713	Site-directed mutagenesis of the three N-linked glycosylation sites was performed to examine the function of individual glycosylation sites in secretion and signal transduction.
30056138	4	5	from	function	659:666	arg1	transduction					726:737	signal transduction	719:737	signal transduction	719:737	Site-directed mutagenesis of the three N-linked glycosylation sites was performed to examine the function of individual glycosylation sites in secretion and signal transduction.
30056138	12	6	theme	EC50	1685:1688	arg1	value					1690:1694	The EC50 value	1681:1694	The EC50 value of the cAMP response at Asn79 of the α-subunit	1681:1741	The EC50 value of the cAMP response at Asn79 of the α-subunit was increased by 5-fold, whereas the maximum response was dramatically reduced to 17.8% of wild-type levels.
30056138	10	7	theme	Enzymatic	1341:1349	arg1	digestion					1351:1359	Enzymatic digestion	1341:1359	Enzymatic digestion of oligosaccharides using PNGaseF treatment	1341:1403	Enzymatic digestion of oligosaccharides using PNGaseF treatment showed that the molecular weights of all mutants, including wild-type, were reduced to 25 kDa.
30056138	5	8	theme	Chinese	815:821	arg1	cells					843:847	Chinese hamster ovary (CHO) cells	815:847	Chinese hamster ovary (CHO) cells	815:847	The absence of the Asn79N-linked sugar chain slightly reduced secretion in Chinese hamster ovary (CHO) cells.
30056138	1	9	theme	hormone	164:170	arg1	β-subunit					181:189	a common α-subunit and hormone specific β-subunit	141:189	β-subunit	181:189	Eel luteinizing hormone (eelLH) is composed of a common α-subunit and hormone specific β-subunit, both of which contain asparagine-linked carbohydrate residues, located at positions 56 and 79 on the α-subunit and position 10 on the β-subunit.
30056138	8	10	theme	chain	1139:1143	arg1	Mutants					1079:1085	Mutants	1079:1085	Mutants (β/α△56, β/α△79, and β△10/α) of the oligosaccharide chain at a single site	1079:1160	Mutants (β/α△56, β/α△79, and β△10/α) of the oligosaccharide chain at a single site showed molecular weights that were reduced by approximately 10%.
30056138	6	11	theme	eelLHβ/α	868:875	arg1	expression					854:863	The expression	850:863	The expression of eelLHβ/α (wild-type) in CHO suspension cells	850:911	The expression of eelLHβ/α (wild-type) in CHO suspension cells was increased by approximately 2-fold higher than that of attached CHO cells.
30056138	0	12	link	N-linked	44:51	arg1	oligosaccharides					53:68	eel luteinizing hormone N-linked oligosaccharides	20:68	eel luteinizing hormone N-linked oligosaccharides	20:68	Site specificity of eel luteinizing hormone N-linked oligosaccharides in signal transduction.
30056138	3	13	theme	eelLH	545:549	arg1	β-subunit					551:559	the eelLH β-subunit	541:559	the eelLH β-subunit	541:559	Thus, we characterized the biologically active single chains by fusing the α-subunit to the carboxyl terminal region of the eelLH β-subunit.
30056138	4	14	theme	sites	624:628	arg1	mutagenesis					576:586	Site-directed mutagenesis	562:586	Site-directed mutagenesis of the three N-linked glycosylation sites	562:628	Site-directed mutagenesis of the three N-linked glycosylation sites was performed to examine the function of individual glycosylation sites in secretion and signal transduction.
30056138	1	15	theme	luteinizing	98:108	arg1	eelLH					119:123	eelLH	119:123	eelLH	119:123	Eel luteinizing hormone (eelLH) is composed of a common α-subunit and hormone specific β-subunit, both of which contain asparagine-linked carbohydrate residues, located at positions 56 and 79 on the α-subunit and position 10 on the β-subunit.
30056138	1	15	theme	luteinizing	98:108	arg1	hormone					110:116	Eel luteinizing hormone	94:116	Eel luteinizing hormone (eelLH)	94:124	Eel luteinizing hormone (eelLH) is composed of a common α-subunit and hormone specific β-subunit, both of which contain asparagine-linked carbohydrate residues, located at positions 56 and 79 on the α-subunit and position 10 on the β-subunit.
30056138	1	16	from	positions	266:274	arg1	α-subunit					293:301	the α-subunit	289:301	the α-subunit	289:301	Eel luteinizing hormone (eelLH) is composed of a common α-subunit and hormone specific β-subunit, both of which contain asparagine-linked carbohydrate residues, located at positions 56 and 79 on the α-subunit and position 10 on the β-subunit.
30056138	1	16	from	positions	266:274	arg1	β-subunit					326:334	the β-subunit	322:334	the β-subunit	322:334	Eel luteinizing hormone (eelLH) is composed of a common α-subunit and hormone specific β-subunit, both of which contain asparagine-linked carbohydrate residues, located at positions 56 and 79 on the α-subunit and position 10 on the β-subunit.
30056138	1	16	from	positions	266:274	arg1	located					255:261	located	255:261	located	255:261	Eel luteinizing hormone (eelLH) is composed of a common α-subunit and hormone specific β-subunit, both of which contain asparagine-linked carbohydrate residues, located at positions 56 and 79 on the α-subunit and position 10 on the β-subunit.
30056138	5	17	theme	CHO	838:840	arg1	cells					843:847	Chinese hamster ovary (CHO) cells	815:847	Chinese hamster ovary (CHO) cells	815:847	The absence of the Asn79N-linked sugar chain slightly reduced secretion in Chinese hamster ovary (CHO) cells.
30056138	12	18	theme	wild-type	1834:1842	arg1	levels					1844:1849	wild-type levels	1834:1849	wild-type levels	1834:1849	The EC50 value of the cAMP response at Asn79 of the α-subunit was increased by 5-fold, whereas the maximum response was dramatically reduced to 17.8% of wild-type levels.
30056138	14	19	theme	carbohydrate	2036:2047	arg1	residues					2049:2056	eelLH carbohydrate residues	2030:2056	eelLH carbohydrate residues	2030:2056	These results revealed the site-specific roles of eelLH carbohydrate residues.
30056138	5	20	theme	hamster	823:829	arg1	cells					843:847	Chinese hamster ovary (CHO) cells	815:847	Chinese hamster ovary (CHO) cells	815:847	The absence of the Asn79N-linked sugar chain slightly reduced secretion in Chinese hamster ovary (CHO) cells.
30056138	7	21	theme	western	994:1000	arg1	analysis					1011:1018	western blotting analysis	994:1018	western blotting analysis	994:1018	By western blotting analysis, the molecular weight of wild-type was found to be 32 kDa.
30056138	8	22	theme	molecular	1169:1177	arg1	weights					1179:1185	molecular weights	1169:1185	molecular weights that were reduced by approximately 10%	1169:1224	Mutants (β/α△56, β/α△79, and β△10/α) of the oligosaccharide chain at a single site showed molecular weights that were reduced by approximately 10%.
30056138	7	23	theme	molecular	1025:1033	arg1	32 kDa					1071:1076	32 kDa	1071:1076	32 kDa	1071:1076	By western blotting analysis, the molecular weight of wild-type was found to be 32 kDa.
30056138	7	23	theme	molecular	1025:1033	arg1	weight					1035:1040	the molecular weight	1021:1040	the molecular weight of wild-type	1021:1053	By western blotting analysis, the molecular weight of wild-type was found to be 32 kDa.
30056138	1	24	contain	contain	206:212	arg1	both					192:195	both	192:195	both	192:195	Eel luteinizing hormone (eelLH) is composed of a common α-subunit and hormone specific β-subunit, both of which contain asparagine-linked carbohydrate residues, located at positions 56 and 79 on the α-subunit and position 10 on the β-subunit.
30056138	1	24	contain	contain	206:212	arg1	β-subunit					181:189	a common α-subunit and hormone specific β-subunit	141:189	β-subunit	181:189	Eel luteinizing hormone (eelLH) is composed of a common α-subunit and hormone specific β-subunit, both of which contain asparagine-linked carbohydrate residues, located at positions 56 and 79 on the α-subunit and position 10 on the β-subunit.
30056138	1	24	contain	contain	206:212	arg2	residues					245:252	asparagine-linked carbohydrate residues	214:252	asparagine-linked carbohydrate residues	214:252	Eel luteinizing hormone (eelLH) is composed of a common α-subunit and hormone specific β-subunit, both of which contain asparagine-linked carbohydrate residues, located at positions 56 and 79 on the α-subunit and position 10 on the β-subunit.
30056138	1	24	contain	contain	206:212	arg1	α-subunit					150:158	a common α-subunit and hormone specific β-subunit	141:189	α-subunit	150:158	Eel luteinizing hormone (eelLH) is composed of a common α-subunit and hormone specific β-subunit, both of which contain asparagine-linked carbohydrate residues, located at positions 56 and 79 on the α-subunit and position 10 on the β-subunit.
30056138	4	25	theme	signal	719:724	arg1	transduction					726:737	signal transduction	719:737	signal transduction	719:737	Site-directed mutagenesis of the three N-linked glycosylation sites was performed to examine the function of individual glycosylation sites in secretion and signal transduction.
30056138	11	26	theme	eelLH	1665:1669	arg1	receptor					1671:1678	the eelLH receptor	1661:1678	the eelLH receptor	1661:1678	The results obtained in the absence of carbohydrates at Asn56 of the α-subunit and at Asn10 of the β-subunit revealed their roles in signal transduction through the eelLH receptor.
30056138	11	27	from	roles	1624:1628	arg1	transduction					1640:1651	signal transduction	1633:1651	signal transduction through the eelLH receptor	1633:1678	The results obtained in the absence of carbohydrates at Asn56 of the α-subunit and at Asn10 of the β-subunit revealed their roles in signal transduction through the eelLH receptor.
30056138	4	28	theme	individual	671:680	arg1	sites					696:700	individual glycosylation sites	671:700	individual glycosylation sites	671:700	Site-directed mutagenesis of the three N-linked glycosylation sites was performed to examine the function of individual glycosylation sites in secretion and signal transduction.
30056138	4	29	theme	sites	696:700	arg1	function					659:666	the function	655:666	the function of individual glycosylation sites in secretion and signal transduction	655:737	Site-directed mutagenesis of the three N-linked glycosylation sites was performed to examine the function of individual glycosylation sites in secretion and signal transduction.
30056138	5	30	theme	sugar	773:777	arg1	chain					779:783	the Asn79N-linked sugar chain	755:783	the Asn79N-linked sugar chain	755:783	The absence of the Asn79N-linked sugar chain slightly reduced secretion in Chinese hamster ovary (CHO) cells.
30056138	15	31	theme	N-linked	2081:2088	arg1	chains					2106:2111	N-linked oligosaccharide chains	2081:2111	N-linked oligosaccharide chains	2081:2111	Our data suggest that N-linked oligosaccharide chains play a pivotal role in biological activity through the eelLH receptor.
30056138	6	32	theme	CHO	980:982	arg1	cells					984:988	attached CHO cells	971:988	attached CHO cells	971:988	The expression of eelLHβ/α (wild-type) in CHO suspension cells was increased by approximately 2-fold higher than that of attached CHO cells.
30056138	9	33	theme	double	1240:1245	arg1	mutant					1247:1252	the double mutant	1236:1252	the double mutant	1236:1252	However, the double mutant (β/α△56.79) molecular weight was decreased by more than 20% compared to the wild-type.
30056138	2	34	theme	carbohydrate	374:385	arg1	chains					387:392	the individual carbohydrate chains	359:392	the individual carbohydrate chains	359:392	The specific roles of the individual carbohydrate chains are poorly defined in eel.
30056138	12	35	theme	maximum	1780:1786	arg1	response					1788:1795	the maximum response	1776:1795	the maximum response	1776:1795	The EC50 value of the cAMP response at Asn79 of the α-subunit was increased by 5-fold, whereas the maximum response was dramatically reduced to 17.8% of wild-type levels.
30056138	0	36	theme	Site	0:3	arg1	specificity					5:15	Site specificity	0:15	Site specificity of eel luteinizing hormone N-linked oligosaccharides in signal transduction.	0:92	Site specificity of eel luteinizing hormone N-linked oligosaccharides in signal transduction.
30056138	1	37	from	79	283:284	arg1	located					255:261	located	255:261	located	255:261	Eel luteinizing hormone (eelLH) is composed of a common α-subunit and hormone specific β-subunit, both of which contain asparagine-linked carbohydrate residues, located at positions 56 and 79 on the α-subunit and position 10 on the β-subunit.
30056138	5	38	theme	ovary	831:835	arg1	cells					843:847	Chinese hamster ovary (CHO) cells	815:847	Chinese hamster ovary (CHO) cells	815:847	The absence of the Asn79N-linked sugar chain slightly reduced secretion in Chinese hamster ovary (CHO) cells.
30056138	15	39	link	N-linked	2081:2088	arg1	chains					2106:2111	N-linked oligosaccharide chains	2081:2111	N-linked oligosaccharide chains	2081:2111	Our data suggest that N-linked oligosaccharide chains play a pivotal role in biological activity through the eelLH receptor.
30056138	4	40	link	N-linked	601:608	arg1	sites					624:628	the three N-linked glycosylation sites	591:628	the three N-linked glycosylation sites	591:628	Site-directed mutagenesis of the three N-linked glycosylation sites was performed to examine the function of individual glycosylation sites in secretion and signal transduction.
30056138	10	41	theme	molecular	1421:1429	arg1	weights					1431:1437	the molecular weights	1417:1437	the molecular weights of all mutants, including wild-type,	1417:1474	Enzymatic digestion of oligosaccharides using PNGaseF treatment showed that the molecular weights of all mutants, including wild-type, were reduced to 25 kDa.
30056138	0	42	theme	luteinizing	24:34	arg1	oligosaccharides					53:68	eel luteinizing hormone N-linked oligosaccharides	20:68	eel luteinizing hormone N-linked oligosaccharides	20:68	Site specificity of eel luteinizing hormone N-linked oligosaccharides in signal transduction.
30056138	11	43	theme	β-subunit	1599:1607	arg1	Asn10					1586:1590	Asn10	1586:1590	Asn10 of the β-subunit	1586:1607	The results obtained in the absence of carbohydrates at Asn56 of the α-subunit and at Asn10 of the β-subunit revealed their roles in signal transduction through the eelLH receptor.
30056138	3	44	theme	single	468:473	arg1	chains					475:480	the biologically active single chains	444:480	the biologically active single chains	444:480	Thus, we characterized the biologically active single chains by fusing the α-subunit to the carboxyl terminal region of the eelLH β-subunit.
30056138	0	45	theme	N-linked	44:51	arg1	oligosaccharides					53:68	eel luteinizing hormone N-linked oligosaccharides	20:68	eel luteinizing hormone N-linked oligosaccharides	20:68	Site specificity of eel luteinizing hormone N-linked oligosaccharides in signal transduction.
30056138	12	46	theme	α-subunit	1733:1741	arg1	Asn79					1720:1724	Asn79	1720:1724	Asn79 of the α-subunit	1720:1741	The EC50 value of the cAMP response at Asn79 of the α-subunit was increased by 5-fold, whereas the maximum response was dramatically reduced to 17.8% of wild-type levels.
30056138	10	47	theme	mutants	1446:1452	arg1	weights					1431:1437	the molecular weights	1417:1437	the molecular weights of all mutants, including wild-type,	1417:1474	Enzymatic digestion of oligosaccharides using PNGaseF treatment showed that the molecular weights of all mutants, including wild-type, were reduced to 25 kDa.
30056138	13	48	theme	carbohydrates	1881:1893	arg1	removal					1866:1872	removal	1866:1872	removal of the carbohydrates at double mutant (β/α△56.79)	1866:1922	Specifically, removal of the carbohydrates at double mutant (β/α△56.79) is approximately 85% to wild-type levels in biopotency.
30056138	1	49	theme	carbohydrate	232:243	arg1	residues					245:252	asparagine-linked carbohydrate residues	214:252	asparagine-linked carbohydrate residues	214:252	Eel luteinizing hormone (eelLH) is composed of a common α-subunit and hormone specific β-subunit, both of which contain asparagine-linked carbohydrate residues, located at positions 56 and 79 on the α-subunit and position 10 on the β-subunit.
30056138	12	50	theme	response	1708:1715	arg1	value					1690:1694	The EC50 value	1681:1694	The EC50 value of the cAMP response at Asn79 of the α-subunit	1681:1741	The EC50 value of the cAMP response at Asn79 of the α-subunit was increased by 5-fold, whereas the maximum response was dramatically reduced to 17.8% of wild-type levels.
30056138	11	51	theme	α-subunit	1569:1577	arg1	Asn56					1556:1560	Asn56	1556:1560	Asn56 of the α-subunit	1556:1577	The results obtained in the absence of carbohydrates at Asn56 of the α-subunit and at Asn10 of the β-subunit revealed their roles in signal transduction through the eelLH receptor.
30056138	13	52	theme	double	1898:1903	arg1	β/α△56.79					1913:1921	β/α△56.79	1913:1921	β/α△56.79	1913:1921	Specifically, removal of the carbohydrates at double mutant (β/α△56.79) is approximately 85% to wild-type levels in biopotency.
30056138	13	52	theme	double	1898:1903	arg1	mutant					1905:1910	double mutant	1898:1910	double mutant (β/α△56.79)	1898:1922	Specifically, removal of the carbohydrates at double mutant (β/α△56.79) is approximately 85% to wild-type levels in biopotency.
30056138	6	53	theme	CHO	892:894	arg1	cells					907:911	CHO suspension cells	892:911	CHO suspension cells	892:911	The expression of eelLHβ/α (wild-type) in CHO suspension cells was increased by approximately 2-fold higher than that of attached CHO cells.
30056138	3	54	theme	carboxyl	513:520	arg1	region					531:536	the carboxyl terminal region	509:536	the carboxyl terminal region of the eelLH β-subunit	509:559	Thus, we characterized the biologically active single chains by fusing the α-subunit to the carboxyl terminal region of the eelLH β-subunit.
30056138	4	55	theme	Site-directed	562:574	arg1	mutagenesis					576:586	Site-directed mutagenesis	562:586	Site-directed mutagenesis of the three N-linked glycosylation sites	562:628	Site-directed mutagenesis of the three N-linked glycosylation sites was performed to examine the function of individual glycosylation sites in secretion and signal transduction.
30056138	4	56	gly	glycosylation	610:622	arg2	three					595:599	three	595:599	three	595:599	Site-directed mutagenesis of the three N-linked glycosylation sites was performed to examine the function of individual glycosylation sites in secretion and signal transduction.
30056138	4	56	gly	glycosylation	610:622	arg2	sites					624:628	the three N-linked glycosylation sites	591:628	the three N-linked glycosylation sites	591:628	Site-directed mutagenesis of the three N-linked glycosylation sites was performed to examine the function of individual glycosylation sites in secretion and signal transduction.
30056138	8	57	from	site	1157:1160	arg1	Mutants					1079:1085	Mutants	1079:1085	Mutants (β/α△56, β/α△79, and β△10/α) of the oligosaccharide chain at a single site	1079:1160	Mutants (β/α△56, β/α△79, and β△10/α) of the oligosaccharide chain at a single site showed molecular weights that were reduced by approximately 10%.
30056138	8	57	from	site	1157:1160	arg1	chain					1139:1143	the oligosaccharide chain	1119:1143	the oligosaccharide chain at a single site	1119:1160	Mutants (β/α△56, β/α△79, and β△10/α) of the oligosaccharide chain at a single site showed molecular weights that were reduced by approximately 10%.
30056138	5	58	link	Asn79N-linked	759:771	arg1	chain					779:783	the Asn79N-linked sugar chain	755:783	the Asn79N-linked sugar chain	755:783	The absence of the Asn79N-linked sugar chain slightly reduced secretion in Chinese hamster ovary (CHO) cells.
30056138	1	59	theme	specific	172:179	arg1	β-subunit					181:189	a common α-subunit and hormone specific β-subunit	141:189	β-subunit	181:189	Eel luteinizing hormone (eelLH) is composed of a common α-subunit and hormone specific β-subunit, both of which contain asparagine-linked carbohydrate residues, located at positions 56 and 79 on the α-subunit and position 10 on the β-subunit.
30056138	8	60	theme	oligosaccharide	1123:1137	arg1	chain					1139:1143	the oligosaccharide chain	1119:1143	the oligosaccharide chain at a single site	1119:1160	Mutants (β/α△56, β/α△79, and β△10/α) of the oligosaccharide chain at a single site showed molecular weights that were reduced by approximately 10%.
30056138	14	61	theme	site-specific	2007:2019	arg1	roles					2021:2025	the site-specific roles	2003:2025	the site-specific roles of eelLH carbohydrate residues	2003:2056	These results revealed the site-specific roles of eelLH carbohydrate residues.
30056138	8	62	dep	Mutants	1079:1085	arg1	β/α△79					1096:1101	β/α△79	1096:1101	β/α△79	1096:1101	Mutants (β/α△56, β/α△79, and β△10/α) of the oligosaccharide chain at a single site showed molecular weights that were reduced by approximately 10%.
30056138	8	62	dep	Mutants	1079:1085	arg1	β△10/α					1108:1113	β△10/α	1108:1113	β△10/α	1108:1113	Mutants (β/α△56, β/α△79, and β△10/α) of the oligosaccharide chain at a single site showed molecular weights that were reduced by approximately 10%.
30056138	8	62	dep	Mutants	1079:1085	arg1	β/α△56					1088:1093	β/α△56	1088:1093	β/α△56	1088:1093	Mutants (β/α△56, β/α△79, and β△10/α) of the oligosaccharide chain at a single site showed molecular weights that were reduced by approximately 10%.
30056138	7	63	theme	wild-type	1045:1053	arg1	32 kDa					1071:1076	32 kDa	1071:1076	32 kDa	1071:1076	By western blotting analysis, the molecular weight of wild-type was found to be 32 kDa.
30056138	7	63	theme	wild-type	1045:1053	arg1	weight					1035:1040	the molecular weight	1021:1040	the molecular weight of wild-type	1021:1053	By western blotting analysis, the molecular weight of wild-type was found to be 32 kDa.
30056138	1	64	link	asparagine-linked	214:230	arg1	residues					245:252	asparagine-linked carbohydrate residues	214:252	asparagine-linked carbohydrate residues	214:252	Eel luteinizing hormone (eelLH) is composed of a common α-subunit and hormone specific β-subunit, both of which contain asparagine-linked carbohydrate residues, located at positions 56 and 79 on the α-subunit and position 10 on the β-subunit.
30056138	4	65	theme	glycosylation	610:622	arg1	sites					624:628	the three N-linked glycosylation sites	591:628	the three N-linked glycosylation sites	591:628	Site-directed mutagenesis of the three N-linked glycosylation sites was performed to examine the function of individual glycosylation sites in secretion and signal transduction.
30056138	13	66	from	levels	1958:1963	arg1	biopotency					1968:1977	biopotency	1968:1977	biopotency	1968:1977	Specifically, removal of the carbohydrates at double mutant (β/α△56.79) is approximately 85% to wild-type levels in biopotency.
30056138	13	67	from	mutant	1905:1910	arg1	removal					1866:1872	removal	1866:1872	removal of the carbohydrates at double mutant (β/α△56.79)	1866:1922	Specifically, removal of the carbohydrates at double mutant (β/α△56.79) is approximately 85% to wild-type levels in biopotency.
30056138	8	68	theme	single	1150:1155	arg1	site					1157:1160	a single site	1148:1160	a single site	1148:1160	Mutants (β/α△56, β/α△79, and β△10/α) of the oligosaccharide chain at a single site showed molecular weights that were reduced by approximately 10%.
30056138	14	69	theme	eelLH	2030:2034	arg1	residues					2049:2056	eelLH carbohydrate residues	2030:2056	eelLH carbohydrate residues	2030:2056	These results revealed the site-specific roles of eelLH carbohydrate residues.
30056138	3	70	theme	β-subunit	551:559	arg1	region					531:536	the carboxyl terminal region	509:536	the carboxyl terminal region of the eelLH β-subunit	509:559	Thus, we characterized the biologically active single chains by fusing the α-subunit to the carboxyl terminal region of the eelLH β-subunit.
30056138	7	71	theme	blotting	1002:1009	arg1	analysis					1011:1018	western blotting analysis	994:1018	western blotting analysis	994:1018	By western blotting analysis, the molecular weight of wild-type was found to be 32 kDa.
30056138	15	72	theme	pivotal	2120:2126	arg1	role					2128:2131	a pivotal role	2118:2131	a pivotal role	2118:2131	Our data suggest that N-linked oligosaccharide chains play a pivotal role in biological activity through the eelLH receptor.
30056138	12	73	theme	levels	1844:1849	arg1	levels					1844:1849	wild-type levels	1834:1849	wild-type levels	1834:1849	The EC50 value of the cAMP response at Asn79 of the α-subunit was increased by 5-fold, whereas the maximum response was dramatically reduced to 17.8% of wild-type levels.
30056138	12	73	theme	levels	1844:1849	arg1	%					1829:1829	17.8%	1825:1829	17.8% of wild-type levels	1825:1849	The EC50 value of the cAMP response at Asn79 of the α-subunit was increased by 5-fold, whereas the maximum response was dramatically reduced to 17.8% of wild-type levels.
30056138	4	74	gly	glycosylation	682:694	arg2	sites					696:700	individual glycosylation sites	671:700	individual glycosylation sites	671:700	Site-directed mutagenesis of the three N-linked glycosylation sites was performed to examine the function of individual glycosylation sites in secretion and signal transduction.
30056138	0	75	theme	hormone	36:42	arg1	oligosaccharides					53:68	eel luteinizing hormone N-linked oligosaccharides	20:68	eel luteinizing hormone N-linked oligosaccharides	20:68	Site specificity of eel luteinizing hormone N-linked oligosaccharides in signal transduction.
30056138	0	76	theme	signal	73:78	arg1	transduction					80:91	signal transduction	73:91	signal transduction	73:91	Site specificity of eel luteinizing hormone N-linked oligosaccharides in signal transduction.
30056138	15	77	theme	oligosaccharide	2090:2104	arg1	chains					2106:2111	N-linked oligosaccharide chains	2081:2111	N-linked oligosaccharide chains	2081:2111	Our data suggest that N-linked oligosaccharide chains play a pivotal role in biological activity through the eelLH receptor.
30056138	8	78	from	Mutants	1079:1085	arg1	site					1157:1160	a single site	1148:1160	a single site	1148:1160	Mutants (β/α△56, β/α△79, and β△10/α) of the oligosaccharide chain at a single site showed molecular weights that were reduced by approximately 10%.
30056138	6	79	from	expression	854:863	arg1	cells					907:911	CHO suspension cells	892:911	CHO suspension cells	892:911	The expression of eelLHβ/α (wild-type) in CHO suspension cells was increased by approximately 2-fold higher than that of attached CHO cells.
30056138	5	80	theme	Asn79N-linked	759:771	arg1	chain					779:783	the Asn79N-linked sugar chain	755:783	the Asn79N-linked sugar chain	755:783	The absence of the Asn79N-linked sugar chain slightly reduced secretion in Chinese hamster ovary (CHO) cells.
30056138	13	81	theme	wild-type	1948:1956	arg1	levels					1958:1963	wild-type levels	1948:1963	wild-type levels in biopotency	1948:1977	Specifically, removal of the carbohydrates at double mutant (β/α△56.79) is approximately 85% to wild-type levels in biopotency.
30056138	1	82	theme	common	143:148	arg1	α-subunit					150:158	a common α-subunit and hormone specific β-subunit	141:189	α-subunit	150:158	Eel luteinizing hormone (eelLH) is composed of a common α-subunit and hormone specific β-subunit, both of which contain asparagine-linked carbohydrate residues, located at positions 56 and 79 on the α-subunit and position 10 on the β-subunit.
30056138	0	83	from	specificity	5:15	arg1	transduction					80:91	signal transduction	73:91	signal transduction	73:91	Site specificity of eel luteinizing hormone N-linked oligosaccharides in signal transduction.
30056138	10	84	theme	oligosaccharides	1364:1379	arg1	digestion					1351:1359	Enzymatic digestion	1341:1359	Enzymatic digestion of oligosaccharides using PNGaseF treatment	1341:1403	Enzymatic digestion of oligosaccharides using PNGaseF treatment showed that the molecular weights of all mutants, including wild-type, were reduced to 25 kDa.
30056138	4	85	theme	glycosylation	682:694	arg1	sites					696:700	individual glycosylation sites	671:700	individual glycosylation sites	671:700	Site-directed mutagenesis of the three N-linked glycosylation sites was performed to examine the function of individual glycosylation sites in secretion and signal transduction.
30056138	6	86	theme	attached	971:978	arg1	cells					984:988	attached CHO cells	971:988	attached CHO cells	971:988	The expression of eelLHβ/α (wild-type) in CHO suspension cells was increased by approximately 2-fold higher than that of attached CHO cells.
30056138	9	87	dep	decreased	1287:1295	arg1	β/α△56.79					1255:1263	β/α△56.79	1255:1263	β/α△56.79	1255:1263	However, the double mutant (β/α△56.79) molecular weight was decreased by more than 20% compared to the wild-type.
30056138	14	88	theme	residues	2049:2056	arg1	roles					2021:2025	the site-specific roles	2003:2025	the site-specific roles of eelLH carbohydrate residues	2003:2056	These results revealed the site-specific roles of eelLH carbohydrate residues.
30056138	2	89	theme	individual	363:372	arg1	chains					387:392	the individual carbohydrate chains	359:392	the individual carbohydrate chains	359:392	The specific roles of the individual carbohydrate chains are poorly defined in eel.
30056138	1	90	theme	Eel	94:96	arg1	eelLH					119:123	eelLH	119:123	eelLH	119:123	Eel luteinizing hormone (eelLH) is composed of a common α-subunit and hormone specific β-subunit, both of which contain asparagine-linked carbohydrate residues, located at positions 56 and 79 on the α-subunit and position 10 on the β-subunit.
30056138	1	90	theme	Eel	94:96	arg1	hormone					110:116	Eel luteinizing hormone	94:116	Eel luteinizing hormone (eelLH)	94:124	Eel luteinizing hormone (eelLH) is composed of a common α-subunit and hormone specific β-subunit, both of which contain asparagine-linked carbohydrate residues, located at positions 56 and 79 on the α-subunit and position 10 on the β-subunit.
30056138	10	91	theme	PNGaseF	1387:1393	arg1	treatment					1395:1403	PNGaseF treatment	1387:1403	PNGaseF treatment	1387:1403	Enzymatic digestion of oligosaccharides using PNGaseF treatment showed that the molecular weights of all mutants, including wild-type, were reduced to 25 kDa.
30056138	0	92	theme	eel	20:22	arg1	oligosaccharides					53:68	eel luteinizing hormone N-linked oligosaccharides	20:68	eel luteinizing hormone N-linked oligosaccharides	20:68	Site specificity of eel luteinizing hormone N-linked oligosaccharides in signal transduction.
30056138	9	93	theme	molecular	1266:1274	arg1	weight					1276:1281	molecular weight	1266:1281	molecular weight	1266:1281	However, the double mutant (β/α△56.79) molecular weight was decreased by more than 20% compared to the wild-type.
30056138	5	94	theme	chain	779:783	arg1	absence					744:750	The absence	740:750	The absence of the Asn79N-linked sugar chain	740:783	The absence of the Asn79N-linked sugar chain slightly reduced secretion in Chinese hamster ovary (CHO) cells.
30056138	15	95	theme	eelLH	2168:2172	arg1	receptor					2174:2181	the eelLH receptor	2164:2181	the eelLH receptor	2164:2181	Our data suggest that N-linked oligosaccharide chains play a pivotal role in biological activity through the eelLH receptor.
30056138	2	96	theme	chains	387:392	arg1	roles					350:354	The specific roles	337:354	The specific roles of the individual carbohydrate chains	337:392	The specific roles of the individual carbohydrate chains are poorly defined in eel.
30056138	0	97	theme	oligosaccharides	53:68	arg1	specificity					5:15	Site specificity	0:15	Site specificity of eel luteinizing hormone N-linked oligosaccharides in signal transduction.	0:92	Site specificity of eel luteinizing hormone N-linked oligosaccharides in signal transduction.
30056138	11	98	theme	signal	1633:1638	arg1	transduction					1640:1651	signal transduction	1633:1651	signal transduction through the eelLH receptor	1633:1678	The results obtained in the absence of carbohydrates at Asn56 of the α-subunit and at Asn10 of the β-subunit revealed their roles in signal transduction through the eelLH receptor.
30056138	15	99	theme	biological	2136:2145	arg1	activity					2147:2154	biological activity	2136:2154	biological activity through the eelLH receptor	2136:2181	Our data suggest that N-linked oligosaccharide chains play a pivotal role in biological activity through the eelLH receptor.
30056138	12	100	from	Asn79	1720:1724	arg1	value					1690:1694	The EC50 value	1681:1694	The EC50 value of the cAMP response at Asn79 of the α-subunit	1681:1741	The EC50 value of the cAMP response at Asn79 of the α-subunit was increased by 5-fold, whereas the maximum response was dramatically reduced to 17.8% of wild-type levels.
30056138	11	101	theme	carbohydrates	1539:1551	arg1	absence					1528:1534	the absence	1524:1534	the absence of carbohydrates	1524:1551	The results obtained in the absence of carbohydrates at Asn56 of the α-subunit and at Asn10 of the β-subunit revealed their roles in signal transduction through the eelLH receptor.
30056138	3	102	theme	active	461:466	arg1	chains					475:480	the biologically active single chains	444:480	the biologically active single chains	444:480	Thus, we characterized the biologically active single chains by fusing the α-subunit to the carboxyl terminal region of the eelLH β-subunit.
30056138	1	103	theme	asparagine-linked	214:230	arg1	residues					245:252	asparagine-linked carbohydrate residues	214:252	asparagine-linked carbohydrate residues	214:252	Eel luteinizing hormone (eelLH) is composed of a common α-subunit and hormone specific β-subunit, both of which contain asparagine-linked carbohydrate residues, located at positions 56 and 79 on the α-subunit and position 10 on the β-subunit.
30056138	4	104	theme	N-linked	601:608	arg1	sites					624:628	the three N-linked glycosylation sites	591:628	the three N-linked glycosylation sites	591:628	Site-directed mutagenesis of the three N-linked glycosylation sites was performed to examine the function of individual glycosylation sites in secretion and signal transduction.
30056138	12	105	dep	increased	1747:1755	arg1	whereas					1768:1774	whereas	1768:1774	whereas	1768:1774	The EC50 value of the cAMP response at Asn79 of the α-subunit was increased by 5-fold, whereas the maximum response was dramatically reduced to 17.8% of wild-type levels.
30056138	2	106	theme	specific	341:348	arg1	roles					350:354	The specific roles	337:354	The specific roles of the individual carbohydrate chains	337:392	The specific roles of the individual carbohydrate chains are poorly defined in eel.
30056138	13	107	attach	removal	1866:1872	arg3	mutant					1905:1910	double mutant	1898:1910	double mutant (β/α△56.79)	1898:1922	Specifically, removal of the carbohydrates at double mutant (β/α△56.79) is approximately 85% to wild-type levels in biopotency.
30056138	13	107	attach	removal	1866:1872	arg3	β/α△56.79					1913:1921	β/α△56.79	1913:1921	β/α△56.79	1913:1921	Specifically, removal of the carbohydrates at double mutant (β/α△56.79) is approximately 85% to wild-type levels in biopotency.
30056138	13	107	attach	removal	1866:1872	arg2	carbohydrates					1881:1893	the carbohydrates	1877:1893	the carbohydrates	1877:1893	Specifically, removal of the carbohydrates at double mutant (β/α△56.79) is approximately 85% to wild-type levels in biopotency.
30094950	5	0	theme	expression	788:797	arg1	levels					799:804	changed expression levels	780:804	changed expression levels	780:804	Their findings indicate glycopeptides with changed expression levels and the presence of altered glycan forms observed in four proteins derived from OSCC patients' sera.
30094950	4	1	theme	squamous	705:712	arg1	OSCC					730:733	OSCC	730:733	OSCC	730:733	In this issue, Saraswat et al. describe mass spectrometry-based investigations of glycopeptide changes in oral cavity squamous cell carcinoma (OSCC).
30094950	4	1	theme	squamous	705:712	arg1	carcinoma					719:727	squamous cell carcinoma	705:727	oral cavity squamous cell carcinoma (OSCC)	693:734	In this issue, Saraswat et al. describe mass spectrometry-based investigations of glycopeptide changes in oral cavity squamous cell carcinoma (OSCC).
30094950	4	2	gly	glycopeptide	669:680	arg2	glycopeptide					669:680	glycopeptide	669:680	glycopeptide	669:680	In this issue, Saraswat et al. describe mass spectrometry-based investigations of glycopeptide changes in oral cavity squamous cell carcinoma (OSCC).
30094950	1	3	theme	modifications	134:146	arg1	modifications					134:146	the most common and complex posttranslational modifications	88:146	the most common and complex posttranslational modifications	88:146	Protein glycosylation, as one of the most common and complex posttranslational modifications, plays an important role in many biological processes.
30094950	1	3	theme	modifications	134:146	arg1	one					81:83	one	81:83	one	81:83	Protein glycosylation, as one of the most common and complex posttranslational modifications, plays an important role in many biological processes.
30094950	8	4	theme	early	1427:1431	arg1	diagnosis					1433:1441	early diagnosis	1427:1441	early diagnosis of patients using mass spectrometry-based approach	1427:1492	Only then, early diagnosis of patients using mass spectrometry-based approach will make sense, as those investigations are very convoluted, and all efforts made during sample preparation and analysis will pay off when comprehensive anti-cancer prevention will be achieved during single measurement.
30094950	6	5	theme	distinctive	930:940	arg1	pattern					942:948	this distinctive pattern	925:948	this distinctive pattern	925:948	Proteins carrying this distinctive pattern are in the group of the most abundant components of serum; IgG1, IgG4, HPT and TRFE, what makes their identification more accessible.
30094950	7	6	theme	cancer	1122:1127	arg1	samples					1135:1141	cancer serum samples	1122:1141	cancer serum samples	1122:1141	Described changes, characteristic for cancer serum samples, may be considered as potential diagnostic indicators of OSCC, however there is still a need to establish a universal glycopeptide-based biomarkers database, where all glycoproteomic data can be collected from all types of cancer studies and evaluated using meta-analyses.
30094950	4	7	theme	cell	714:717	arg1	OSCC					730:733	OSCC	730:733	OSCC	730:733	In this issue, Saraswat et al. describe mass spectrometry-based investigations of glycopeptide changes in oral cavity squamous cell carcinoma (OSCC).
30094950	4	7	theme	cell	714:717	arg1	carcinoma					719:727	squamous cell carcinoma	705:727	oral cavity squamous cell carcinoma (OSCC)	693:734	In this issue, Saraswat et al. describe mass spectrometry-based investigations of glycopeptide changes in oral cavity squamous cell carcinoma (OSCC).
30094950	8	8	theme	single	1695:1700	arg1	measurement					1702:1712	single measurement	1695:1712	single measurement	1695:1712	Only then, early diagnosis of patients using mass spectrometry-based approach will make sense, as those investigations are very convoluted, and all efforts made during sample preparation and analysis will pay off when comprehensive anti-cancer prevention will be achieved during single measurement.
30094950	7	9	theme	Described	1084:1092	arg1	changes					1094:1100	Described changes	1084:1100	Described changes	1084:1100	Described changes, characteristic for cancer serum samples, may be considered as potential diagnostic indicators of OSCC, however there is still a need to establish a universal glycopeptide-based biomarkers database, where all glycoproteomic data can be collected from all types of cancer studies and evaluated using meta-analyses.
30094950	7	9	theme	Described	1084:1092	arg1	indicators					1186:1195	potential diagnostic indicators	1165:1195	potential diagnostic indicators of OSCC	1165:1203	Described changes, characteristic for cancer serum samples, may be considered as potential diagnostic indicators of OSCC, however there is still a need to establish a universal glycopeptide-based biomarkers database, where all glycoproteomic data can be collected from all types of cancer studies and evaluated using meta-analyses.
30094950	5	10	located	observed	847:854	arg1	proteins					864:871	four proteins	859:871	four proteins derived from OSCC patients' sera	859:904	Their findings indicate glycopeptides with changed expression levels and the presence of altered glycan forms observed in four proteins derived from OSCC patients' sera.
30094950	5	10	located	observed	847:854	arg2	levels					799:804	changed expression levels	780:804	changed expression levels	780:804	Their findings indicate glycopeptides with changed expression levels and the presence of altered glycan forms observed in four proteins derived from OSCC patients' sera.
30094950	3	11	theme	glycosylation	532:544	arg1	patterns					546:553	glycosylation patterns	532:553	glycosylation patterns	532:553	The possibility of simultaneous identification of amino acid sequence, glycosylation sites, and glycan composition enabled the monitoring of changes in glycosylation patterns in various pathological states.
30094950	3	12	from	patterns	546:553	arg1	monitoring					507:516	the monitoring	503:516	the monitoring of changes in glycosylation patterns in various pathological states	503:584	The possibility of simultaneous identification of amino acid sequence, glycosylation sites, and glycan composition enabled the monitoring of changes in glycosylation patterns in various pathological states.
30094950	6	13	theme	serum	1002:1006	arg1	IgG4					1015:1018	IgG4	1015:1018	IgG4	1015:1018	Proteins carrying this distinctive pattern are in the group of the most abundant components of serum; IgG1, IgG4, HPT and TRFE, what makes their identification more accessible.
30094950	6	13	theme	serum	1002:1006	arg1	IgG1					1009:1012	IgG1	1009:1012	IgG1	1009:1012	Proteins carrying this distinctive pattern are in the group of the most abundant components of serum; IgG1, IgG4, HPT and TRFE, what makes their identification more accessible.
30094950	6	13	theme	serum	1002:1006	arg1	TRFE					1029:1032	TRFE	1029:1032	TRFE	1029:1032	Proteins carrying this distinctive pattern are in the group of the most abundant components of serum; IgG1, IgG4, HPT and TRFE, what makes their identification more accessible.
30094950	6	13	theme	serum	1002:1006	arg1	HPT					1021:1023	HPT	1021:1023	HPT	1021:1023	Proteins carrying this distinctive pattern are in the group of the most abundant components of serum; IgG1, IgG4, HPT and TRFE, what makes their identification more accessible.
30094950	6	13	theme	serum	1002:1006	arg1	components					988:997	the most abundant components	970:997	the most abundant components of serum; IgG1, IgG4, HPT and TRFE	970:1032	Proteins carrying this distinctive pattern are in the group of the most abundant components of serum; IgG1, IgG4, HPT and TRFE, what makes their identification more accessible.
30094950	3	14	theme	pathological	566:577	arg1	states					579:584	various pathological states	558:584	various pathological states	558:584	The possibility of simultaneous identification of amino acid sequence, glycosylation sites, and glycan composition enabled the monitoring of changes in glycosylation patterns in various pathological states.
30094950	7	15	theme	characteristic	1103:1116	arg1	changes					1094:1100	Described changes	1084:1100	Described changes	1084:1100	Described changes, characteristic for cancer serum samples, may be considered as potential diagnostic indicators of OSCC, however there is still a need to establish a universal glycopeptide-based biomarkers database, where all glycoproteomic data can be collected from all types of cancer studies and evaluated using meta-analyses.
30094950	7	15	theme	characteristic	1103:1116	arg1	indicators					1186:1195	potential diagnostic indicators	1165:1195	potential diagnostic indicators of OSCC	1165:1203	Described changes, characteristic for cancer serum samples, may be considered as potential diagnostic indicators of OSCC, however there is still a need to establish a universal glycopeptide-based biomarkers database, where all glycoproteomic data can be collected from all types of cancer studies and evaluated using meta-analyses.
30094950	3	16	from	monitoring	507:516	arg1	patterns					546:553	glycosylation patterns	532:553	glycosylation patterns	532:553	The possibility of simultaneous identification of amino acid sequence, glycosylation sites, and glycan composition enabled the monitoring of changes in glycosylation patterns in various pathological states.
30094950	3	16	from	monitoring	507:516	arg1	states					579:584	various pathological states	558:584	various pathological states	558:584	The possibility of simultaneous identification of amino acid sequence, glycosylation sites, and glycan composition enabled the monitoring of changes in glycosylation patterns in various pathological states.
30094950	7	17	theme	universal	1251:1259	arg1	database					1291:1298	a universal glycopeptide-based biomarkers database	1249:1298	a universal glycopeptide-based biomarkers database	1249:1298	Described changes, characteristic for cancer serum samples, may be considered as potential diagnostic indicators of OSCC, however there is still a need to establish a universal glycopeptide-based biomarkers database, where all glycoproteomic data can be collected from all types of cancer studies and evaluated using meta-analyses.
30094950	7	18	theme	studies	1373:1379	arg1	types					1357:1361	all types	1353:1361	all types of cancer studies	1353:1379	Described changes, characteristic for cancer serum samples, may be considered as potential diagnostic indicators of OSCC, however there is still a need to establish a universal glycopeptide-based biomarkers database, where all glycoproteomic data can be collected from all types of cancer studies and evaluated using meta-analyses.
30094950	3	19	theme	sites	465:469	arg1	identification					412:425	simultaneous identification	399:425	simultaneous identification of amino acid sequence, glycosylation sites, and glycan composition	399:493	The possibility of simultaneous identification of amino acid sequence, glycosylation sites, and glycan composition enabled the monitoring of changes in glycosylation patterns in various pathological states.
30094950	1	20	theme	Protein	55:61	arg1	glycosylation					63:75	Protein glycosylation	55:75	Protein glycosylation	55:75	Protein glycosylation, as one of the most common and complex posttranslational modifications, plays an important role in many biological processes.
30094950	3	21	theme	identification	412:425	arg1	possibility					384:394	The possibility	380:394	The possibility of simultaneous identification of amino acid sequence, glycosylation sites, and glycan composition	380:493	The possibility of simultaneous identification of amino acid sequence, glycosylation sites, and glycan composition enabled the monitoring of changes in glycosylation patterns in various pathological states.
30094950	3	22	theme	amino	430:434	arg1	sequence					441:448	amino acid sequence	430:448	amino acid sequence	430:448	The possibility of simultaneous identification of amino acid sequence, glycosylation sites, and glycan composition enabled the monitoring of changes in glycosylation patterns in various pathological states.
30094950	7	23	theme	potential	1165:1173	arg1	changes					1094:1100	Described changes	1084:1100	Described changes	1084:1100	Described changes, characteristic for cancer serum samples, may be considered as potential diagnostic indicators of OSCC, however there is still a need to establish a universal glycopeptide-based biomarkers database, where all glycoproteomic data can be collected from all types of cancer studies and evaluated using meta-analyses.
30094950	7	23	theme	potential	1165:1173	arg1	indicators					1186:1195	potential diagnostic indicators	1165:1195	potential diagnostic indicators of OSCC	1165:1203	Described changes, characteristic for cancer serum samples, may be considered as potential diagnostic indicators of OSCC, however there is still a need to establish a universal glycopeptide-based biomarkers database, where all glycoproteomic data can be collected from all types of cancer studies and evaluated using meta-analyses.
30094950	2	24	theme	spectrometry	245:256	arg1	techniques					258:267	mass spectrometry techniques	240:267	mass spectrometry techniques	240:267	Along with the intensive progress in mass spectrometry techniques and development of glycan search tools and databases, glycoproteomics has become a popular subject of studies.
30094950	4	25	from	changes	682:688	arg1	cavity					698:703	oral cavity squamous cell carcinoma (OSCC)	693:734	oral cavity squamous cell carcinoma (OSCC)	693:734	In this issue, Saraswat et al. describe mass spectrometry-based investigations of glycopeptide changes in oral cavity squamous cell carcinoma (OSCC).
30094950	2	26	theme	studies	371:377	arg1	subject					360:366	a popular subject	350:366	a popular subject of studies	350:377	Along with the intensive progress in mass spectrometry techniques and development of glycan search tools and databases, glycoproteomics has become a popular subject of studies.
30094950	5	27	theme	altered	826:832	arg1	forms					841:845	altered glycan forms	826:845	altered glycan forms	826:845	Their findings indicate glycopeptides with changed expression levels and the presence of altered glycan forms observed in four proteins derived from OSCC patients' sera.
30094950	7	28	theme	biomarkers	1280:1289	arg1	database					1291:1298	a universal glycopeptide-based biomarkers database	1249:1298	a universal glycopeptide-based biomarkers database	1249:1298	Described changes, characteristic for cancer serum samples, may be considered as potential diagnostic indicators of OSCC, however there is still a need to establish a universal glycopeptide-based biomarkers database, where all glycoproteomic data can be collected from all types of cancer studies and evaluated using meta-analyses.
30094950	4	29	theme	glycopeptide	669:680	arg1	changes					682:688	glycopeptide changes	669:688	glycopeptide changes in oral cavity squamous cell carcinoma (OSCC)	669:734	In this issue, Saraswat et al. describe mass spectrometry-based investigations of glycopeptide changes in oral cavity squamous cell carcinoma (OSCC).
30094950	2	30	theme	tools	302:306	arg1	development					273:283	development	273:283	development of glycan search tools and databases	273:320	Along with the intensive progress in mass spectrometry techniques and development of glycan search tools and databases, glycoproteomics has become a popular subject of studies.
30094950	2	30	theme	tools	302:306	arg1	techniques					258:267	mass spectrometry techniques	240:267	mass spectrometry techniques	240:267	Along with the intensive progress in mass spectrometry techniques and development of glycan search tools and databases, glycoproteomics has become a popular subject of studies.
30094950	1	31	theme	important	158:166	arg1	role					168:171	an important role	155:171	an important role	155:171	Protein glycosylation, as one of the most common and complex posttranslational modifications, plays an important role in many biological processes.
30094950	5	32	theme	glycan	834:839	arg1	forms					841:845	altered glycan forms	826:845	altered glycan forms	826:845	Their findings indicate glycopeptides with changed expression levels and the presence of altered glycan forms observed in four proteins derived from OSCC patients' sera.
30094950	6	33	theme	components	988:997	arg1	IgG1					1009:1012	IgG1	1009:1012	IgG1	1009:1012	Proteins carrying this distinctive pattern are in the group of the most abundant components of serum; IgG1, IgG4, HPT and TRFE, what makes their identification more accessible.
30094950	6	33	theme	components	988:997	arg1	TRFE					1029:1032	TRFE	1029:1032	TRFE	1029:1032	Proteins carrying this distinctive pattern are in the group of the most abundant components of serum; IgG1, IgG4, HPT and TRFE, what makes their identification more accessible.
30094950	6	33	theme	components	988:997	arg1	components					988:997	the most abundant components	970:997	the most abundant components of serum; IgG1, IgG4, HPT and TRFE	970:1032	Proteins carrying this distinctive pattern are in the group of the most abundant components of serum; IgG1, IgG4, HPT and TRFE, what makes their identification more accessible.
30094950	6	33	theme	components	988:997	arg1	IgG4					1015:1018	IgG4	1015:1018	IgG4	1015:1018	Proteins carrying this distinctive pattern are in the group of the most abundant components of serum; IgG1, IgG4, HPT and TRFE, what makes their identification more accessible.
30094950	6	33	theme	components	988:997	arg1	HPT					1021:1023	HPT	1021:1023	HPT	1021:1023	Proteins carrying this distinctive pattern are in the group of the most abundant components of serum; IgG1, IgG4, HPT and TRFE, what makes their identification more accessible.
30094950	6	33	theme	components	988:997	arg1	group					961:965	the group	957:965	the group of the most abundant components of serum; IgG1, IgG4, HPT and TRFE	957:1032	Proteins carrying this distinctive pattern are in the group of the most abundant components of serum; IgG1, IgG4, HPT and TRFE, what makes their identification more accessible.
30094950	8	34	theme	spectrometry-based	1466:1483	arg1	approach					1485:1492	mass spectrometry-based approach	1461:1492	mass spectrometry-based approach	1461:1492	Only then, early diagnosis of patients using mass spectrometry-based approach will make sense, as those investigations are very convoluted, and all efforts made during sample preparation and analysis will pay off when comprehensive anti-cancer prevention will be achieved during single measurement.
30094950	4	35	theme	changes	682:688	arg1	investigations					651:664	mass spectrometry-based investigations	627:664	mass spectrometry-based investigations of glycopeptide changes in oral cavity squamous cell carcinoma (OSCC)	627:734	In this issue, Saraswat et al. describe mass spectrometry-based investigations of glycopeptide changes in oral cavity squamous cell carcinoma (OSCC).
30094950	2	36	theme	search	295:300	arg1	tools					302:306	glycan search tools	288:306	glycan search tools	288:306	Along with the intensive progress in mass spectrometry techniques and development of glycan search tools and databases, glycoproteomics has become a popular subject of studies.
30094950	2	37	from	progress	228:235	arg1	development					273:283	development	273:283	development of glycan search tools and databases	273:320	Along with the intensive progress in mass spectrometry techniques and development of glycan search tools and databases, glycoproteomics has become a popular subject of studies.
30094950	2	37	from	progress	228:235	arg1	techniques					258:267	mass spectrometry techniques	240:267	mass spectrometry techniques	240:267	Along with the intensive progress in mass spectrometry techniques and development of glycan search tools and databases, glycoproteomics has become a popular subject of studies.
30094950	7	38	theme	glycoproteomic	1311:1324	arg1	data					1326:1329	all glycoproteomic data	1307:1329	all glycoproteomic data	1307:1329	Described changes, characteristic for cancer serum samples, may be considered as potential diagnostic indicators of OSCC, however there is still a need to establish a universal glycopeptide-based biomarkers database, where all glycoproteomic data can be collected from all types of cancer studies and evaluated using meta-analyses.
30094950	5	39	theme	forms	841:845	arg1	presence					814:821	the presence	810:821	the presence of altered glycan forms	810:845	Their findings indicate glycopeptides with changed expression levels and the presence of altered glycan forms observed in four proteins derived from OSCC patients' sera.
30094950	5	39	theme	forms	841:845	arg1	levels					799:804	changed expression levels	780:804	changed expression levels	780:804	Their findings indicate glycopeptides with changed expression levels and the presence of altered glycan forms observed in four proteins derived from OSCC patients' sera.
30094950	6	40	theme	abundant	979:986	arg1	IgG4					1015:1018	IgG4	1015:1018	IgG4	1015:1018	Proteins carrying this distinctive pattern are in the group of the most abundant components of serum; IgG1, IgG4, HPT and TRFE, what makes their identification more accessible.
30094950	6	40	theme	abundant	979:986	arg1	IgG1					1009:1012	IgG1	1009:1012	IgG1	1009:1012	Proteins carrying this distinctive pattern are in the group of the most abundant components of serum; IgG1, IgG4, HPT and TRFE, what makes their identification more accessible.
30094950	6	40	theme	abundant	979:986	arg1	TRFE					1029:1032	TRFE	1029:1032	TRFE	1029:1032	Proteins carrying this distinctive pattern are in the group of the most abundant components of serum; IgG1, IgG4, HPT and TRFE, what makes their identification more accessible.
30094950	6	40	theme	abundant	979:986	arg1	HPT					1021:1023	HPT	1021:1023	HPT	1021:1023	Proteins carrying this distinctive pattern are in the group of the most abundant components of serum; IgG1, IgG4, HPT and TRFE, what makes their identification more accessible.
30094950	6	40	theme	abundant	979:986	arg1	components					988:997	the most abundant components	970:997	the most abundant components of serum; IgG1, IgG4, HPT and TRFE	970:1032	Proteins carrying this distinctive pattern are in the group of the most abundant components of serum; IgG1, IgG4, HPT and TRFE, what makes their identification more accessible.
30094950	2	41	theme	glycan	288:293	arg1	tools					302:306	glycan search tools	288:306	glycan search tools	288:306	Along with the intensive progress in mass spectrometry techniques and development of glycan search tools and databases, glycoproteomics has become a popular subject of studies.
30094950	4	42	from	investigations	651:664	arg1	cavity					698:703	oral cavity squamous cell carcinoma (OSCC)	693:734	oral cavity squamous cell carcinoma (OSCC)	693:734	In this issue, Saraswat et al. describe mass spectrometry-based investigations of glycopeptide changes in oral cavity squamous cell carcinoma (OSCC).
30094950	3	43	theme	glycosylation	451:463	arg1	sites					465:469	glycosylation sites	451:469	glycosylation sites	451:469	The possibility of simultaneous identification of amino acid sequence, glycosylation sites, and glycan composition enabled the monitoring of changes in glycosylation patterns in various pathological states.
30094950	3	44	theme	changes	521:527	arg1	monitoring					507:516	the monitoring	503:516	the monitoring of changes in glycosylation patterns in various pathological states	503:584	The possibility of simultaneous identification of amino acid sequence, glycosylation sites, and glycan composition enabled the monitoring of changes in glycosylation patterns in various pathological states.
30094950	3	45	theme	various	558:564	arg1	states					579:584	various pathological states	558:584	various pathological states	558:584	The possibility of simultaneous identification of amino acid sequence, glycosylation sites, and glycan composition enabled the monitoring of changes in glycosylation patterns in various pathological states.
30094950	1	46	theme	many	176:179	arg1	processes					192:200	many biological processes	176:200	many biological processes	176:200	Protein glycosylation, as one of the most common and complex posttranslational modifications, plays an important role in many biological processes.
30094950	0	47	theme	Early	28:32	arg1	Detection					34:42	Early Detection	28:42	Early Detection of Cancer	28:52	Glycopeptides as a Tool for Early Detection of Cancer.
30094950	4	48	theme	oral	693:696	arg1	cavity					698:703	oral cavity squamous cell carcinoma (OSCC)	693:734	oral cavity squamous cell carcinoma (OSCC)	693:734	In this issue, Saraswat et al. describe mass spectrometry-based investigations of glycopeptide changes in oral cavity squamous cell carcinoma (OSCC).
30094950	3	49	theme	sequence	441:448	arg1	identification					412:425	simultaneous identification	399:425	simultaneous identification of amino acid sequence, glycosylation sites, and glycan composition	399:493	The possibility of simultaneous identification of amino acid sequence, glycosylation sites, and glycan composition enabled the monitoring of changes in glycosylation patterns in various pathological states.
30094950	7	50	theme	cancer	1366:1371	arg1	studies					1373:1379	cancer studies	1366:1379	cancer studies	1366:1379	Described changes, characteristic for cancer serum samples, may be considered as potential diagnostic indicators of OSCC, however there is still a need to establish a universal glycopeptide-based biomarkers database, where all glycoproteomic data can be collected from all types of cancer studies and evaluated using meta-analyses.
30094950	1	51	theme	biological	181:190	arg1	processes					192:200	many biological processes	176:200	many biological processes	176:200	Protein glycosylation, as one of the most common and complex posttranslational modifications, plays an important role in many biological processes.
30094950	3	52	theme	simultaneous	399:410	arg1	identification					412:425	simultaneous identification	399:425	simultaneous identification of amino acid sequence, glycosylation sites, and glycan composition	399:493	The possibility of simultaneous identification of amino acid sequence, glycosylation sites, and glycan composition enabled the monitoring of changes in glycosylation patterns in various pathological states.
30094950	5	53	theme	OSCC	886:889	arg1	sera					901:904	OSCC patients' sera	886:904	OSCC patients' sera	886:904	Their findings indicate glycopeptides with changed expression levels and the presence of altered glycan forms observed in four proteins derived from OSCC patients' sera.
30094950	8	54	theme	patients	1446:1453	arg1	diagnosis					1433:1441	early diagnosis	1427:1441	early diagnosis of patients using mass spectrometry-based approach	1427:1492	Only then, early diagnosis of patients using mass spectrometry-based approach will make sense, as those investigations are very convoluted, and all efforts made during sample preparation and analysis will pay off when comprehensive anti-cancer prevention will be achieved during single measurement.
30094950	3	55	gly	glycosylation	451:463	arg2	sites					465:469	glycosylation sites	451:469	glycosylation sites	451:469	The possibility of simultaneous identification of amino acid sequence, glycosylation sites, and glycan composition enabled the monitoring of changes in glycosylation patterns in various pathological states.
30094950	6	56	contain	carrying	916:923	arg1	Proteins					907:914	Proteins	907:914	Proteins carrying this distinctive pattern	907:948	Proteins carrying this distinctive pattern are in the group of the most abundant components of serum; IgG1, IgG4, HPT and TRFE, what makes their identification more accessible.
30094950	6	56	contain	carrying	916:923	arg2	pattern					942:948	this distinctive pattern	925:948	this distinctive pattern	925:948	Proteins carrying this distinctive pattern are in the group of the most abundant components of serum; IgG1, IgG4, HPT and TRFE, what makes their identification more accessible.
30094950	7	57	theme	serum	1129:1133	arg1	samples					1135:1141	cancer serum samples	1122:1141	cancer serum samples	1122:1141	Described changes, characteristic for cancer serum samples, may be considered as potential diagnostic indicators of OSCC, however there is still a need to establish a universal glycopeptide-based biomarkers database, where all glycoproteomic data can be collected from all types of cancer studies and evaluated using meta-analyses.
30094950	8	58	theme	sample	1584:1589	arg1	preparation					1591:1601	sample preparation	1584:1601	sample preparation	1584:1601	Only then, early diagnosis of patients using mass spectrometry-based approach will make sense, as those investigations are very convoluted, and all efforts made during sample preparation and analysis will pay off when comprehensive anti-cancer prevention will be achieved during single measurement.
30094950	5	59	theme	changed	780:786	arg1	levels					799:804	changed expression levels	780:804	changed expression levels	780:804	Their findings indicate glycopeptides with changed expression levels and the presence of altered glycan forms observed in four proteins derived from OSCC patients' sera.
30094950	3	60	theme	glycan	476:481	arg1	composition					483:493	glycan composition	476:493	glycan composition	476:493	The possibility of simultaneous identification of amino acid sequence, glycosylation sites, and glycan composition enabled the monitoring of changes in glycosylation patterns in various pathological states.
30094950	8	61	theme	comprehensive	1634:1646	arg1	prevention					1660:1669	comprehensive anti-cancer prevention	1634:1669	comprehensive anti-cancer prevention	1634:1669	Only then, early diagnosis of patients using mass spectrometry-based approach will make sense, as those investigations are very convoluted, and all efforts made during sample preparation and analysis will pay off when comprehensive anti-cancer prevention will be achieved during single measurement.
30094950	8	62	theme	mass	1461:1464	arg1	approach					1485:1492	mass spectrometry-based approach	1461:1492	mass spectrometry-based approach	1461:1492	Only then, early diagnosis of patients using mass spectrometry-based approach will make sense, as those investigations are very convoluted, and all efforts made during sample preparation and analysis will pay off when comprehensive anti-cancer prevention will be achieved during single measurement.
30094950	3	63	theme	composition	483:493	arg1	identification					412:425	simultaneous identification	399:425	simultaneous identification of amino acid sequence, glycosylation sites, and glycan composition	399:493	The possibility of simultaneous identification of amino acid sequence, glycosylation sites, and glycan composition enabled the monitoring of changes in glycosylation patterns in various pathological states.
30094950	8	64	theme	anti-cancer	1648:1658	arg1	prevention					1660:1669	comprehensive anti-cancer prevention	1634:1669	comprehensive anti-cancer prevention	1634:1669	Only then, early diagnosis of patients using mass spectrometry-based approach will make sense, as those investigations are very convoluted, and all efforts made during sample preparation and analysis will pay off when comprehensive anti-cancer prevention will be achieved during single measurement.
30094950	7	65	theme	glycopeptide-based	1261:1278	arg1	database					1291:1298	a universal glycopeptide-based biomarkers database	1249:1298	a universal glycopeptide-based biomarkers database	1249:1298	Described changes, characteristic for cancer serum samples, may be considered as potential diagnostic indicators of OSCC, however there is still a need to establish a universal glycopeptide-based biomarkers database, where all glycoproteomic data can be collected from all types of cancer studies and evaluated using meta-analyses.
30094950	7	66	dep	potential	1165:1173	arg1	diagnostic					1175:1184	diagnostic	1175:1184	diagnostic	1175:1184	Described changes, characteristic for cancer serum samples, may be considered as potential diagnostic indicators of OSCC, however there is still a need to establish a universal glycopeptide-based biomarkers database, where all glycoproteomic data can be collected from all types of cancer studies and evaluated using meta-analyses.
30094950	1	67	theme	complex	108:114	arg1	modifications					134:146	the most common and complex posttranslational modifications	88:146	the most common and complex posttranslational modifications	88:146	Protein glycosylation, as one of the most common and complex posttranslational modifications, plays an important role in many biological processes.
30094950	2	68	theme	mass	240:243	arg1	techniques					258:267	mass spectrometry techniques	240:267	mass spectrometry techniques	240:267	Along with the intensive progress in mass spectrometry techniques and development of glycan search tools and databases, glycoproteomics has become a popular subject of studies.
30094950	5	69	with	glycopeptides	761:773	arg1	presence					814:821	the presence	810:821	the presence of altered glycan forms	810:845	Their findings indicate glycopeptides with changed expression levels and the presence of altered glycan forms observed in four proteins derived from OSCC patients' sera.
30094950	5	69	with	glycopeptides	761:773	arg1	levels					799:804	changed expression levels	780:804	changed expression levels	780:804	Their findings indicate glycopeptides with changed expression levels and the presence of altered glycan forms observed in four proteins derived from OSCC patients' sera.
30094950	1	70	theme	common	97:102	arg1	modifications					134:146	the most common and complex posttranslational modifications	88:146	the most common and complex posttranslational modifications	88:146	Protein glycosylation, as one of the most common and complex posttranslational modifications, plays an important role in many biological processes.
30094950	5	71	gly	glycopeptides	761:773	arg2	glycopeptides					761:773	glycopeptides	761:773	glycopeptides with changed expression levels and the presence of altered glycan forms observed in four proteins derived from OSCC patients' sera	761:904	Their findings indicate glycopeptides with changed expression levels and the presence of altered glycan forms observed in four proteins derived from OSCC patients' sera.
30094950	4	72	dep	cavity	698:703	arg1	OSCC					730:733	OSCC	730:733	OSCC	730:733	In this issue, Saraswat et al. describe mass spectrometry-based investigations of glycopeptide changes in oral cavity squamous cell carcinoma (OSCC).
30094950	4	72	dep	cavity	698:703	arg1	carcinoma					719:727	squamous cell carcinoma	705:727	oral cavity squamous cell carcinoma (OSCC)	693:734	In this issue, Saraswat et al. describe mass spectrometry-based investigations of glycopeptide changes in oral cavity squamous cell carcinoma (OSCC).
30094950	6	73	dep	components	988:997	arg1	IgG4					1015:1018	IgG4	1015:1018	IgG4	1015:1018	Proteins carrying this distinctive pattern are in the group of the most abundant components of serum; IgG1, IgG4, HPT and TRFE, what makes their identification more accessible.
30094950	6	73	dep	components	988:997	arg1	IgG1					1009:1012	IgG1	1009:1012	IgG1	1009:1012	Proteins carrying this distinctive pattern are in the group of the most abundant components of serum; IgG1, IgG4, HPT and TRFE, what makes their identification more accessible.
30094950	6	73	dep	components	988:997	arg1	components					988:997	the most abundant components	970:997	the most abundant components of serum; IgG1, IgG4, HPT and TRFE	970:1032	Proteins carrying this distinctive pattern are in the group of the most abundant components of serum; IgG1, IgG4, HPT and TRFE, what makes their identification more accessible.
30094950	6	73	dep	components	988:997	arg1	TRFE					1029:1032	TRFE	1029:1032	TRFE	1029:1032	Proteins carrying this distinctive pattern are in the group of the most abundant components of serum; IgG1, IgG4, HPT and TRFE, what makes their identification more accessible.
30094950	6	73	dep	components	988:997	arg1	HPT					1021:1023	HPT	1021:1023	HPT	1021:1023	Proteins carrying this distinctive pattern are in the group of the most abundant components of serum; IgG1, IgG4, HPT and TRFE, what makes their identification more accessible.
30094950	2	74	theme	databases	312:320	arg1	development					273:283	development	273:283	development of glycan search tools and databases	273:320	Along with the intensive progress in mass spectrometry techniques and development of glycan search tools and databases, glycoproteomics has become a popular subject of studies.
30094950	2	74	theme	databases	312:320	arg1	techniques					258:267	mass spectrometry techniques	240:267	mass spectrometry techniques	240:267	Along with the intensive progress in mass spectrometry techniques and development of glycan search tools and databases, glycoproteomics has become a popular subject of studies.
30094950	4	75	from	cavity	698:703	arg1	investigations					651:664	mass spectrometry-based investigations	627:664	mass spectrometry-based investigations of glycopeptide changes in oral cavity squamous cell carcinoma (OSCC)	627:734	In this issue, Saraswat et al. describe mass spectrometry-based investigations of glycopeptide changes in oral cavity squamous cell carcinoma (OSCC).
30094950	4	76	theme	spectrometry-based	632:649	arg1	investigations					651:664	mass spectrometry-based investigations	627:664	mass spectrometry-based investigations of glycopeptide changes in oral cavity squamous cell carcinoma (OSCC)	627:734	In this issue, Saraswat et al. describe mass spectrometry-based investigations of glycopeptide changes in oral cavity squamous cell carcinoma (OSCC).
30094950	2	77	theme	popular	352:358	arg1	subject					360:366	a popular subject	350:366	a popular subject of studies	350:377	Along with the intensive progress in mass spectrometry techniques and development of glycan search tools and databases, glycoproteomics has become a popular subject of studies.
30094950	3	78	theme	acid	436:439	arg1	sequence					441:448	amino acid sequence	430:448	amino acid sequence	430:448	The possibility of simultaneous identification of amino acid sequence, glycosylation sites, and glycan composition enabled the monitoring of changes in glycosylation patterns in various pathological states.
30094950	0	79	theme	Cancer	47:52	arg1	Detection					34:42	Early Detection	28:42	Early Detection of Cancer	28:52	Glycopeptides as a Tool for Early Detection of Cancer.
30094950	2	80	theme	intensive	218:226	arg1	progress					228:235	the intensive progress	214:235	the intensive progress in mass spectrometry techniques and development of glycan search tools and databases	214:320	Along with the intensive progress in mass spectrometry techniques and development of glycan search tools and databases, glycoproteomics has become a popular subject of studies.
30094950	1	81	theme	posttranslational	116:132	arg1	modifications					134:146	the most common and complex posttranslational modifications	88:146	the most common and complex posttranslational modifications	88:146	Protein glycosylation, as one of the most common and complex posttranslational modifications, plays an important role in many biological processes.
30094950	3	82	from	changes	521:527	arg1	patterns					546:553	glycosylation patterns	532:553	glycosylation patterns	532:553	The possibility of simultaneous identification of amino acid sequence, glycosylation sites, and glycan composition enabled the monitoring of changes in glycosylation patterns in various pathological states.
30094950	7	83	theme	OSCC	1200:1203	arg1	changes					1094:1100	Described changes	1084:1100	Described changes	1084:1100	Described changes, characteristic for cancer serum samples, may be considered as potential diagnostic indicators of OSCC, however there is still a need to establish a universal glycopeptide-based biomarkers database, where all glycoproteomic data can be collected from all types of cancer studies and evaluated using meta-analyses.
30094950	7	83	theme	OSCC	1200:1203	arg1	indicators					1186:1195	potential diagnostic indicators	1165:1195	potential diagnostic indicators of OSCC	1165:1203	Described changes, characteristic for cancer serum samples, may be considered as potential diagnostic indicators of OSCC, however there is still a need to establish a universal glycopeptide-based biomarkers database, where all glycoproteomic data can be collected from all types of cancer studies and evaluated using meta-analyses.
29351928	9	0	theme	T	1601:1601	arg1	cells					1603:1607	activated T cells	1591:1607	activated T cells	1591:1607	Notably, we observed that the transcription factors c-JUN and JUNB show higher levels of O-GlcNAc glycosylation and higher levels of expression in activated T cells.
29351928	5	1	gly	glycopeptides	1029:1041	arg2	glycopeptides					1029:1041	2219 intact O-linked glycopeptides	1008:1041	2219 intact O-linked glycopeptides across 1045 glycoproteins	1008:1067	This approach led to the identification of 2219 intact O-linked glycopeptides across 1045 glycoproteins.
29351928	10	2	theme	O-GlcNAc	1675:1682	arg1	glycoproteins					1684:1696	O-GlcNAc glycoproteins	1675:1696	O-GlcNAc glycoproteins	1675:1696	Overall, our findings provide a quantitative characterization of O-GlcNAc glycoproteins and their corresponding modification sites in primary human T cells, which will facilitate mechanistic studies into the function of O-GlcNAc in T cell activation.
29351928	4	3	theme	resting	863:869	arg1	cells					901:905	resting and activated primary human T cells	863:905	resting and activated primary human T cells	863:905	Thus, to advance insight into the role of O-GlcNAc in T cell activation, we performed glycosite mapping studies via direct glycopeptide measurement on resting and activated primary human T cells with a technique termed Isotope Targeted Glycoproteomics.
29351928	6	4	theme	known	1163:1167	arg1	site					1185:1188	a known phosphorylation site	1161:1188	a known phosphorylation site	1161:1188	A significant proportion (>45%) of the identified O-GlcNAc sites lie near or coincide with a known phosphorylation site, supporting the potential for PTM crosstalk.
29351928	1	5	theme	cells	262:266	arg1	example					319:325	a classic example	309:325	a classic example	309:325	Post-translational modifications (PTMs) on proteins often function to regulate signaling cascades, with the activation of T cells during an adaptive immune response being a classic example.
29351928	1	5	theme	cells	262:266	arg1	activation					246:255	the activation	242:255	the activation of T cells during an adaptive immune response	242:301	Post-translational modifications (PTMs) on proteins often function to regulate signaling cascades, with the activation of T cells during an adaptive immune response being a classic example.
29351928	2	6	located	found	460:464	arg1	proteins					493:500	nuclear and cytoplasmic proteins	469:500	nuclear and cytoplasmic proteins	469:500	Mounting evidence indicates that the modification of proteins by O-linked N-acetylglucosamine (O-GlcNAc), the only mammalian glycan found on nuclear and cytoplasmic proteins, helps regulate T cell activation.
29351928	2	6	located	found	460:464	arg2	glycan					453:458	the only mammalian glycan	434:458	the only mammalian glycan found on nuclear and cytoplasmic proteins	434:500	Mounting evidence indicates that the modification of proteins by O-linked N-acetylglucosamine (O-GlcNAc), the only mammalian glycan found on nuclear and cytoplasmic proteins, helps regulate T cell activation.
29351928	2	6	located	found	460:464	arg2	N-acetylglucosamine					402:420	O-linked N-acetylglucosamine	393:420	O-linked N-acetylglucosamine (O-GlcNAc)	393:431	Mounting evidence indicates that the modification of proteins by O-linked N-acetylglucosamine (O-GlcNAc), the only mammalian glycan found on nuclear and cytoplasmic proteins, helps regulate T cell activation.
29351928	10	7	theme	corresponding	1708:1720	arg1	sites					1735:1739	their corresponding modification sites	1702:1739	their corresponding modification sites in primary human T cells, which will facilitate mechanistic studies into the function of O-GlcNAc in T cell activation	1702:1858	Overall, our findings provide a quantitative characterization of O-GlcNAc glycoproteins and their corresponding modification sites in primary human T cells, which will facilitate mechanistic studies into the function of O-GlcNAc in T cell activation.
29351928	10	7	theme	corresponding	1708:1720	arg1	glycoproteins					1684:1696	O-GlcNAc glycoproteins	1675:1696	O-GlcNAc glycoproteins	1675:1696	Overall, our findings provide a quantitative characterization of O-GlcNAc glycoproteins and their corresponding modification sites in primary human T cells, which will facilitate mechanistic studies into the function of O-GlcNAc in T cell activation.
29351928	4	8	theme	direct	828:833	arg1	measurement					848:858	direct glycopeptide measurement	828:858	direct glycopeptide measurement on resting and activated primary human T cells with a technique termed Isotope Targeted Glycoproteomics	828:962	Thus, to advance insight into the role of O-GlcNAc in T cell activation, we performed glycosite mapping studies via direct glycopeptide measurement on resting and activated primary human T cells with a technique termed Isotope Targeted Glycoproteomics.
29351928	0	9	link	O-linked	40:47	arg1	Glycopeptides					49:61	Over 2000 O-linked Glycopeptides	30:61	Over 2000 O-linked Glycopeptides	30:61	Mapping and Quantification of Over 2000 O-linked Glycopeptides in Activated Human T Cells with Isotope-Targeted Glycoproteomics (Isotag).
29351928	10	10	theme	mechanistic	1789:1799	arg1	studies					1801:1807	mechanistic studies	1789:1807	mechanistic studies into the function of O-GlcNAc in T cell activation	1789:1858	Overall, our findings provide a quantitative characterization of O-GlcNAc glycoproteins and their corresponding modification sites in primary human T cells, which will facilitate mechanistic studies into the function of O-GlcNAc in T cell activation.
29351928	10	11	theme	T	1758:1758	arg1	cells					1760:1764	primary human T cells	1744:1764	primary human T cells	1744:1764	Overall, our findings provide a quantitative characterization of O-GlcNAc glycoproteins and their corresponding modification sites in primary human T cells, which will facilitate mechanistic studies into the function of O-GlcNAc in T cell activation.
29351928	3	12	theme	mechanistic	544:554	arg1	understanding					556:568	a mechanistic understanding	542:568	a mechanistic understanding of how O-GlcNAc functions in T cell activation	542:615	Yet, a mechanistic understanding of how O-GlcNAc functions in T cell activation remains elusive, partly because of the difficulties in mapping and quantifying O-GlcNAc sites.
29351928	6	13	theme	PTM	1220:1222	arg1	crosstalk					1224:1232	PTM crosstalk	1220:1232	PTM crosstalk	1220:1232	A significant proportion (>45%) of the identified O-GlcNAc sites lie near or coincide with a known phosphorylation site, supporting the potential for PTM crosstalk.
29351928	1	14	theme	signaling	217:225	arg1	cascades					227:234	signaling cascades	217:234	signaling cascades	217:234	Post-translational modifications (PTMs) on proteins often function to regulate signaling cascades, with the activation of T cells during an adaptive immune response being a classic example.
29351928	4	15	theme	activated	875:883	arg1	cells					901:905	resting and activated primary human T cells	863:905	resting and activated primary human T cells	863:905	Thus, to advance insight into the role of O-GlcNAc in T cell activation, we performed glycosite mapping studies via direct glycopeptide measurement on resting and activated primary human T cells with a technique termed Isotope Targeted Glycoproteomics.
29351928	8	16	theme	mass	1414:1417	arg1	tags					1419:1422	mass tags	1414:1422	mass tags	1414:1422	To validate our results, we employed gel shift assays based on conjugating mass tags to O-GlcNAc groups.
29351928	5	17	theme	glycopeptides	1029:1041	arg1	identification					990:1003	the identification	986:1003	the identification of 2219 intact O-linked glycopeptides across 1045 glycoproteins	986:1067	This approach led to the identification of 2219 intact O-linked glycopeptides across 1045 glycoproteins.
29351928	9	18	from	cells	1603:1607	arg1	levels					1523:1528	higher levels	1516:1528	higher levels of O-GlcNAc glycosylation	1516:1554	Notably, we observed that the transcription factors c-JUN and JUNB show higher levels of O-GlcNAc glycosylation and higher levels of expression in activated T cells.
29351928	9	18	from	cells	1603:1607	arg1	levels					1567:1572	higher levels	1560:1572	higher levels of expression in activated T cells	1560:1607	Notably, we observed that the transcription factors c-JUN and JUNB show higher levels of O-GlcNAc glycosylation and higher levels of expression in activated T cells.
29351928	4	19	theme	human	893:897	arg1	cells					901:905	resting and activated primary human T cells	863:905	resting and activated primary human T cells	863:905	Thus, to advance insight into the role of O-GlcNAc in T cell activation, we performed glycosite mapping studies via direct glycopeptide measurement on resting and activated primary human T cells with a technique termed Isotope Targeted Glycoproteomics.
29351928	7	20	theme	consensus	1319:1327	arg1	sequence					1329:1336	a strict consensus sequence	1310:1336	a strict consensus sequence	1310:1336	Consistent with other studies, we find that O-GlcNAc sites in T cells lack a strict consensus sequence.
29351928	5	21	theme	1045	1050:1053	arg1	glycoproteins					1055:1067	1045 glycoproteins	1050:1067	1045 glycoproteins	1050:1067	This approach led to the identification of 2219 intact O-linked glycopeptides across 1045 glycoproteins.
29351928	4	22	with	measurement	848:858	arg1	technique					914:922	a technique	912:922	a technique termed Isotope Targeted Glycoproteomics	912:962	Thus, to advance insight into the role of O-GlcNAc in T cell activation, we performed glycosite mapping studies via direct glycopeptide measurement on resting and activated primary human T cells with a technique termed Isotope Targeted Glycoproteomics.
29351928	3	23	theme	cell	601:604	arg1	activation					606:615	T cell activation	599:615	T cell activation	599:615	Yet, a mechanistic understanding of how O-GlcNAc functions in T cell activation remains elusive, partly because of the difficulties in mapping and quantifying O-GlcNAc sites.
29351928	2	24	theme	cytoplasmic	481:491	arg1	proteins					493:500	nuclear and cytoplasmic proteins	469:500	nuclear and cytoplasmic proteins	469:500	Mounting evidence indicates that the modification of proteins by O-linked N-acetylglucosamine (O-GlcNAc), the only mammalian glycan found on nuclear and cytoplasmic proteins, helps regulate T cell activation.
29351928	0	25	from	Quantification	12:25	arg1	Cells					84:88	Activated Human T Cells	66:88	Activated Human T Cells with Isotope-Targeted Glycoproteomics (Isotag)	66:135	Mapping and Quantification of Over 2000 O-linked Glycopeptides in Activated Human T Cells with Isotope-Targeted Glycoproteomics (Isotag).
29351928	0	26	theme	Human	76:80	arg1	Cells					84:88	Activated Human T Cells	66:88	Activated Human T Cells with Isotope-Targeted Glycoproteomics (Isotag)	66:135	Mapping and Quantification of Over 2000 O-linked Glycopeptides in Activated Human T Cells with Isotope-Targeted Glycoproteomics (Isotag).
29351928	4	27	from	measurement	848:858	arg1	cells					901:905	resting and activated primary human T cells	863:905	resting and activated primary human T cells	863:905	Thus, to advance insight into the role of O-GlcNAc in T cell activation, we performed glycosite mapping studies via direct glycopeptide measurement on resting and activated primary human T cells with a technique termed Isotope Targeted Glycoproteomics.
29351928	2	28	theme	cell	520:523	arg1	activation					525:534	T cell activation	518:534	T cell activation	518:534	Mounting evidence indicates that the modification of proteins by O-linked N-acetylglucosamine (O-GlcNAc), the only mammalian glycan found on nuclear and cytoplasmic proteins, helps regulate T cell activation.
29351928	0	29	from	Mapping	0:6	arg1	Cells					84:88	Activated Human T Cells	66:88	Activated Human T Cells with Isotope-Targeted Glycoproteomics (Isotag)	66:135	Mapping and Quantification of Over 2000 O-linked Glycopeptides in Activated Human T Cells with Isotope-Targeted Glycoproteomics (Isotag).
29351928	4	30	from	role	746:749	arg1	activation					773:782	T cell activation	766:782	T cell activation	766:782	Thus, to advance insight into the role of O-GlcNAc in T cell activation, we performed glycosite mapping studies via direct glycopeptide measurement on resting and activated primary human T cells with a technique termed Isotope Targeted Glycoproteomics.
29351928	4	31	theme	Targeted	939:946	arg1	Glycoproteomics					948:962	Isotope Targeted Glycoproteomics	931:962	Isotope Targeted Glycoproteomics	931:962	Thus, to advance insight into the role of O-GlcNAc in T cell activation, we performed glycosite mapping studies via direct glycopeptide measurement on resting and activated primary human T cells with a technique termed Isotope Targeted Glycoproteomics.
29351928	4	32	theme	O-GlcNAc	754:761	arg1	role					746:749	the role	742:749	the role of O-GlcNAc in T cell activation	742:782	Thus, to advance insight into the role of O-GlcNAc in T cell activation, we performed glycosite mapping studies via direct glycopeptide measurement on resting and activated primary human T cells with a technique termed Isotope Targeted Glycoproteomics.
29351928	2	33	theme	O-linked	393:400	arg1	O-GlcNAc					423:430	O-GlcNAc	423:430	O-GlcNAc	423:430	Mounting evidence indicates that the modification of proteins by O-linked N-acetylglucosamine (O-GlcNAc), the only mammalian glycan found on nuclear and cytoplasmic proteins, helps regulate T cell activation.
29351928	2	33	theme	O-linked	393:400	arg1	glycan					453:458	the only mammalian glycan	434:458	the only mammalian glycan found on nuclear and cytoplasmic proteins	434:500	Mounting evidence indicates that the modification of proteins by O-linked N-acetylglucosamine (O-GlcNAc), the only mammalian glycan found on nuclear and cytoplasmic proteins, helps regulate T cell activation.
29351928	2	33	theme	O-linked	393:400	arg1	N-acetylglucosamine					402:420	O-linked N-acetylglucosamine	393:420	O-linked N-acetylglucosamine (O-GlcNAc)	393:431	Mounting evidence indicates that the modification of proteins by O-linked N-acetylglucosamine (O-GlcNAc), the only mammalian glycan found on nuclear and cytoplasmic proteins, helps regulate T cell activation.
29351928	0	34	theme	Isotope-Targeted	95:110	arg1	Isotag					129:134	Isotag	129:134	Isotag	129:134	Mapping and Quantification of Over 2000 O-linked Glycopeptides in Activated Human T Cells with Isotope-Targeted Glycoproteomics (Isotag).
29351928	0	34	theme	Isotope-Targeted	95:110	arg1	Glycoproteomics					112:126	Isotope-Targeted Glycoproteomics	95:126	Isotope-Targeted Glycoproteomics (Isotag)	95:135	Mapping and Quantification of Over 2000 O-linked Glycopeptides in Activated Human T Cells with Isotope-Targeted Glycoproteomics (Isotag).
29351928	6	35	theme	significant	1072:1082	arg1	%					1099:1099	>45%	1096:1099	>45%	1096:1099	A significant proportion (>45%) of the identified O-GlcNAc sites lie near or coincide with a known phosphorylation site, supporting the potential for PTM crosstalk.
29351928	6	35	theme	significant	1072:1082	arg1	proportion					1084:1093	A significant proportion	1070:1093	A significant proportion (>45%) of the identified O-GlcNAc sites	1070:1133	A significant proportion (>45%) of the identified O-GlcNAc sites lie near or coincide with a known phosphorylation site, supporting the potential for PTM crosstalk.
29351928	2	36	theme	mammalian	443:451	arg1	N-acetylglucosamine					402:420	O-linked N-acetylglucosamine	393:420	O-linked N-acetylglucosamine (O-GlcNAc)	393:431	Mounting evidence indicates that the modification of proteins by O-linked N-acetylglucosamine (O-GlcNAc), the only mammalian glycan found on nuclear and cytoplasmic proteins, helps regulate T cell activation.
29351928	2	36	theme	mammalian	443:451	arg1	glycan					453:458	the only mammalian glycan	434:458	the only mammalian glycan found on nuclear and cytoplasmic proteins	434:500	Mounting evidence indicates that the modification of proteins by O-linked N-acetylglucosamine (O-GlcNAc), the only mammalian glycan found on nuclear and cytoplasmic proteins, helps regulate T cell activation.
29351928	2	37	theme	proteins	381:388	arg1	modification					365:376	the modification	361:376	the modification of proteins by O-linked N-acetylglucosamine (O-GlcNAc), the only mammalian glycan found on nuclear and cytoplasmic proteins,	361:501	Mounting evidence indicates that the modification of proteins by O-linked N-acetylglucosamine (O-GlcNAc), the only mammalian glycan found on nuclear and cytoplasmic proteins, helps regulate T cell activation.
29351928	7	38	theme	T	1297:1297	arg1	cells					1299:1303	T cells	1297:1303	T cells	1297:1303	Consistent with other studies, we find that O-GlcNAc sites in T cells lack a strict consensus sequence.
29351928	10	39	from	characterization	1655:1670	arg1	cells					1760:1764	primary human T cells	1744:1764	primary human T cells	1744:1764	Overall, our findings provide a quantitative characterization of O-GlcNAc glycoproteins and their corresponding modification sites in primary human T cells, which will facilitate mechanistic studies into the function of O-GlcNAc in T cell activation.
29351928	4	40	gly	glycosite	798:806	arg2	glycosite					798:806	glycosite mapping studies	798:822	glycosite mapping studies	798:822	Thus, to advance insight into the role of O-GlcNAc in T cell activation, we performed glycosite mapping studies via direct glycopeptide measurement on resting and activated primary human T cells with a technique termed Isotope Targeted Glycoproteomics.
29351928	1	41	theme	adaptive	278:285	arg1	response					294:301	an adaptive immune response	275:301	an adaptive immune response	275:301	Post-translational modifications (PTMs) on proteins often function to regulate signaling cascades, with the activation of T cells during an adaptive immune response being a classic example.
29351928	9	42	theme	O-GlcNAc	1533:1540	arg1	glycosylation					1542:1554	O-GlcNAc glycosylation	1533:1554	O-GlcNAc glycosylation	1533:1554	Notably, we observed that the transcription factors c-JUN and JUNB show higher levels of O-GlcNAc glycosylation and higher levels of expression in activated T cells.
29351928	2	43	theme	nuclear	469:475	arg1	proteins					493:500	nuclear and cytoplasmic proteins	469:500	nuclear and cytoplasmic proteins	469:500	Mounting evidence indicates that the modification of proteins by O-linked N-acetylglucosamine (O-GlcNAc), the only mammalian glycan found on nuclear and cytoplasmic proteins, helps regulate T cell activation.
29351928	10	44	theme	O-GlcNAc	1830:1837	arg1	function					1818:1825	the function	1814:1825	the function of O-GlcNAc in T cell activation	1814:1858	Overall, our findings provide a quantitative characterization of O-GlcNAc glycoproteins and their corresponding modification sites in primary human T cells, which will facilitate mechanistic studies into the function of O-GlcNAc in T cell activation.
29351928	10	45	from	sites	1735:1739	arg1	cells					1760:1764	primary human T cells	1744:1764	primary human T cells	1744:1764	Overall, our findings provide a quantitative characterization of O-GlcNAc glycoproteins and their corresponding modification sites in primary human T cells, which will facilitate mechanistic studies into the function of O-GlcNAc in T cell activation.
29351928	4	46	theme	glycosite	798:806	arg1	studies					816:822	glycosite mapping studies	798:822	glycosite mapping studies	798:822	Thus, to advance insight into the role of O-GlcNAc in T cell activation, we performed glycosite mapping studies via direct glycopeptide measurement on resting and activated primary human T cells with a technique termed Isotope Targeted Glycoproteomics.
29351928	0	47	theme	O-linked	40:47	arg1	Glycopeptides					49:61	Over 2000 O-linked Glycopeptides	30:61	Over 2000 O-linked Glycopeptides	30:61	Mapping and Quantification of Over 2000 O-linked Glycopeptides in Activated Human T Cells with Isotope-Targeted Glycoproteomics (Isotag).
29351928	5	48	theme	O-linked	1020:1027	arg1	glycopeptides					1029:1041	2219 intact O-linked glycopeptides	1008:1041	2219 intact O-linked glycopeptides across 1045 glycoproteins	1008:1067	This approach led to the identification of 2219 intact O-linked glycopeptides across 1045 glycoproteins.
29351928	6	49	theme	identified	1109:1118	arg1	sites					1129:1133	the identified O-GlcNAc sites	1105:1133	the identified O-GlcNAc sites	1105:1133	A significant proportion (>45%) of the identified O-GlcNAc sites lie near or coincide with a known phosphorylation site, supporting the potential for PTM crosstalk.
29351928	6	50	theme	phosphorylation	1169:1183	arg1	site					1185:1188	a known phosphorylation site	1161:1188	a known phosphorylation site	1161:1188	A significant proportion (>45%) of the identified O-GlcNAc sites lie near or coincide with a known phosphorylation site, supporting the potential for PTM crosstalk.
29351928	8	51	theme	shift	1380:1384	arg1	assays					1386:1391	gel shift assays	1376:1391	gel shift assays	1376:1391	To validate our results, we employed gel shift assays based on conjugating mass tags to O-GlcNAc groups.
29351928	10	52	theme	quantitative	1642:1653	arg1	characterization					1655:1670	a quantitative characterization	1640:1670	a quantitative characterization of O-GlcNAc glycoproteins	1640:1696	Overall, our findings provide a quantitative characterization of O-GlcNAc glycoproteins and their corresponding modification sites in primary human T cells, which will facilitate mechanistic studies into the function of O-GlcNAc in T cell activation.
29351928	9	53	theme	expression	1577:1586	arg1	levels					1523:1528	higher levels	1516:1528	higher levels of O-GlcNAc glycosylation	1516:1554	Notably, we observed that the transcription factors c-JUN and JUNB show higher levels of O-GlcNAc glycosylation and higher levels of expression in activated T cells.
29351928	9	53	theme	expression	1577:1586	arg1	levels					1567:1572	higher levels	1560:1572	higher levels of expression in activated T cells	1560:1607	Notably, we observed that the transcription factors c-JUN and JUNB show higher levels of O-GlcNAc glycosylation and higher levels of expression in activated T cells.
29351928	10	54	theme	T	1842:1842	arg1	activation					1849:1858	T cell activation	1842:1858	T cell activation	1842:1858	Overall, our findings provide a quantitative characterization of O-GlcNAc glycoproteins and their corresponding modification sites in primary human T cells, which will facilitate mechanistic studies into the function of O-GlcNAc in T cell activation.
29351928	10	55	from	function	1818:1825	arg1	activation					1849:1858	T cell activation	1842:1858	T cell activation	1842:1858	Overall, our findings provide a quantitative characterization of O-GlcNAc glycoproteins and their corresponding modification sites in primary human T cells, which will facilitate mechanistic studies into the function of O-GlcNAc in T cell activation.
29351928	9	56	theme	activated	1591:1599	arg1	cells					1603:1607	activated T cells	1591:1607	activated T cells	1591:1607	Notably, we observed that the transcription factors c-JUN and JUNB show higher levels of O-GlcNAc glycosylation and higher levels of expression in activated T cells.
29351928	3	57	from	difficulties	656:667	arg1	mapping					672:678	mapping	672:678	mapping	672:678	Yet, a mechanistic understanding of how O-GlcNAc functions in T cell activation remains elusive, partly because of the difficulties in mapping and quantifying O-GlcNAc sites.
29351928	3	57	from	difficulties	656:667	arg1	sites					705:709	quantifying O-GlcNAc sites	684:709	quantifying O-GlcNAc sites	684:709	Yet, a mechanistic understanding of how O-GlcNAc functions in T cell activation remains elusive, partly because of the difficulties in mapping and quantifying O-GlcNAc sites.
29351928	3	58	theme	O-GlcNAc	696:703	arg1	sites					705:709	quantifying O-GlcNAc sites	684:709	quantifying O-GlcNAc sites	684:709	Yet, a mechanistic understanding of how O-GlcNAc functions in T cell activation remains elusive, partly because of the difficulties in mapping and quantifying O-GlcNAc sites.
29351928	10	59	theme	glycoproteins	1684:1696	arg1	characterization					1655:1670	a quantitative characterization	1640:1670	a quantitative characterization of O-GlcNAc glycoproteins	1640:1696	Overall, our findings provide a quantitative characterization of O-GlcNAc glycoproteins and their corresponding modification sites in primary human T cells, which will facilitate mechanistic studies into the function of O-GlcNAc in T cell activation.
29351928	10	59	theme	glycoproteins	1684:1696	arg1	sites					1735:1739	their corresponding modification sites	1702:1739	their corresponding modification sites in primary human T cells, which will facilitate mechanistic studies into the function of O-GlcNAc in T cell activation	1702:1858	Overall, our findings provide a quantitative characterization of O-GlcNAc glycoproteins and their corresponding modification sites in primary human T cells, which will facilitate mechanistic studies into the function of O-GlcNAc in T cell activation.
29351928	10	59	theme	glycoproteins	1684:1696	arg1	glycoproteins					1684:1696	O-GlcNAc glycoproteins	1675:1696	O-GlcNAc glycoproteins	1675:1696	Overall, our findings provide a quantitative characterization of O-GlcNAc glycoproteins and their corresponding modification sites in primary human T cells, which will facilitate mechanistic studies into the function of O-GlcNAc in T cell activation.
29351928	4	60	theme	glycopeptide	835:846	arg1	measurement					848:858	direct glycopeptide measurement	828:858	direct glycopeptide measurement on resting and activated primary human T cells with a technique termed Isotope Targeted Glycoproteomics	828:962	Thus, to advance insight into the role of O-GlcNAc in T cell activation, we performed glycosite mapping studies via direct glycopeptide measurement on resting and activated primary human T cells with a technique termed Isotope Targeted Glycoproteomics.
29351928	5	61	gly	glycoproteins	1055:1067	arg1	glycoproteins					1055:1067	1045 glycoproteins	1050:1067	1045 glycoproteins	1050:1067	This approach led to the identification of 2219 intact O-linked glycopeptides across 1045 glycoproteins.
29351928	7	62	with	Consistent	1235:1244	arg1	studies					1257:1263	other studies	1251:1263	other studies	1251:1263	Consistent with other studies, we find that O-GlcNAc sites in T cells lack a strict consensus sequence.
29351928	9	63	theme	higher	1560:1565	arg1	levels					1567:1572	higher levels	1560:1572	higher levels of expression in activated T cells	1560:1607	Notably, we observed that the transcription factors c-JUN and JUNB show higher levels of O-GlcNAc glycosylation and higher levels of expression in activated T cells.
29351928	1	64	theme	T	260:260	arg1	cells					262:266	T cells	260:266	T cells	260:266	Post-translational modifications (PTMs) on proteins often function to regulate signaling cascades, with the activation of T cells during an adaptive immune response being a classic example.
29351928	10	65	theme	modification	1722:1733	arg1	sites					1735:1739	their corresponding modification sites	1702:1739	their corresponding modification sites in primary human T cells, which will facilitate mechanistic studies into the function of O-GlcNAc in T cell activation	1702:1858	Overall, our findings provide a quantitative characterization of O-GlcNAc glycoproteins and their corresponding modification sites in primary human T cells, which will facilitate mechanistic studies into the function of O-GlcNAc in T cell activation.
29351928	10	65	theme	modification	1722:1733	arg1	glycoproteins					1684:1696	O-GlcNAc glycoproteins	1675:1696	O-GlcNAc glycoproteins	1675:1696	Overall, our findings provide a quantitative characterization of O-GlcNAc glycoproteins and their corresponding modification sites in primary human T cells, which will facilitate mechanistic studies into the function of O-GlcNAc in T cell activation.
29351928	4	66	gly	glycopeptide	835:846	arg2	glycopeptide					835:846	direct glycopeptide measurement	828:858	direct glycopeptide measurement on resting and activated primary human T cells with a technique termed Isotope Targeted Glycoproteomics	828:962	Thus, to advance insight into the role of O-GlcNAc in T cell activation, we performed glycosite mapping studies via direct glycopeptide measurement on resting and activated primary human T cells with a technique termed Isotope Targeted Glycoproteomics.
29351928	4	67	theme	mapping	808:814	arg1	studies					816:822	glycosite mapping studies	798:822	glycosite mapping studies	798:822	Thus, to advance insight into the role of O-GlcNAc in T cell activation, we performed glycosite mapping studies via direct glycopeptide measurement on resting and activated primary human T cells with a technique termed Isotope Targeted Glycoproteomics.
29351928	0	68	with	Cells	84:88	arg1	Isotag					129:134	Isotag	129:134	Isotag	129:134	Mapping and Quantification of Over 2000 O-linked Glycopeptides in Activated Human T Cells with Isotope-Targeted Glycoproteomics (Isotag).
29351928	0	68	with	Cells	84:88	arg1	Glycoproteomics					112:126	Isotope-Targeted Glycoproteomics	95:126	Isotope-Targeted Glycoproteomics (Isotag)	95:135	Mapping and Quantification of Over 2000 O-linked Glycopeptides in Activated Human T Cells with Isotope-Targeted Glycoproteomics (Isotag).
29351928	10	69	theme	human	1752:1756	arg1	cells					1760:1764	primary human T cells	1744:1764	primary human T cells	1744:1764	Overall, our findings provide a quantitative characterization of O-GlcNAc glycoproteins and their corresponding modification sites in primary human T cells, which will facilitate mechanistic studies into the function of O-GlcNAc in T cell activation.
29351928	4	70	theme	T	899:899	arg1	cells					901:905	resting and activated primary human T cells	863:905	resting and activated primary human T cells	863:905	Thus, to advance insight into the role of O-GlcNAc in T cell activation, we performed glycosite mapping studies via direct glycopeptide measurement on resting and activated primary human T cells with a technique termed Isotope Targeted Glycoproteomics.
29351928	6	71	theme	sites	1129:1133	arg1	%					1099:1099	>45%	1096:1099	>45%	1096:1099	A significant proportion (>45%) of the identified O-GlcNAc sites lie near or coincide with a known phosphorylation site, supporting the potential for PTM crosstalk.
29351928	6	71	theme	sites	1129:1133	arg1	proportion					1084:1093	A significant proportion	1070:1093	A significant proportion (>45%) of the identified O-GlcNAc sites	1070:1133	A significant proportion (>45%) of the identified O-GlcNAc sites lie near or coincide with a known phosphorylation site, supporting the potential for PTM crosstalk.
29351928	5	72	link	O-linked	1020:1027	arg1	glycopeptides					1029:1041	2219 intact O-linked glycopeptides	1008:1041	2219 intact O-linked glycopeptides across 1045 glycoproteins	1008:1067	This approach led to the identification of 2219 intact O-linked glycopeptides across 1045 glycoproteins.
29351928	8	73	theme	O-GlcNAc	1427:1434	arg1	groups					1436:1441	O-GlcNAc groups	1427:1441	O-GlcNAc groups	1427:1441	To validate our results, we employed gel shift assays based on conjugating mass tags to O-GlcNAc groups.
29351928	4	74	theme	T	766:766	arg1	activation					773:782	T cell activation	766:782	T cell activation	766:782	Thus, to advance insight into the role of O-GlcNAc in T cell activation, we performed glycosite mapping studies via direct glycopeptide measurement on resting and activated primary human T cells with a technique termed Isotope Targeted Glycoproteomics.
29351928	4	75	theme	primary	885:891	arg1	cells					901:905	resting and activated primary human T cells	863:905	resting and activated primary human T cells	863:905	Thus, to advance insight into the role of O-GlcNAc in T cell activation, we performed glycosite mapping studies via direct glycopeptide measurement on resting and activated primary human T cells with a technique termed Isotope Targeted Glycoproteomics.
29351928	0	76	theme	Activated	66:74	arg1	Cells					84:88	Activated Human T Cells	66:88	Activated Human T Cells with Isotope-Targeted Glycoproteomics (Isotag)	66:135	Mapping and Quantification of Over 2000 O-linked Glycopeptides in Activated Human T Cells with Isotope-Targeted Glycoproteomics (Isotag).
29351928	3	77	theme	T	599:599	arg1	activation					606:615	T cell activation	599:615	T cell activation	599:615	Yet, a mechanistic understanding of how O-GlcNAc functions in T cell activation remains elusive, partly because of the difficulties in mapping and quantifying O-GlcNAc sites.
29351928	1	78	theme	Post-translational	138:155	arg1	modifications					157:169	Post-translational modifications	138:169	Post-translational modifications (PTMs) on proteins	138:188	Post-translational modifications (PTMs) on proteins often function to regulate signaling cascades, with the activation of T cells during an adaptive immune response being a classic example.
29351928	1	78	theme	Post-translational	138:155	arg1	PTMs					172:175	PTMs	172:175	PTMs	172:175	Post-translational modifications (PTMs) on proteins often function to regulate signaling cascades, with the activation of T cells during an adaptive immune response being a classic example.
29351928	9	79	from	levels	1523:1528	arg1	cells					1603:1607	activated T cells	1591:1607	activated T cells	1591:1607	Notably, we observed that the transcription factors c-JUN and JUNB show higher levels of O-GlcNAc glycosylation and higher levels of expression in activated T cells.
29351928	7	80	theme	strict	1312:1317	arg1	sequence					1329:1336	a strict consensus sequence	1310:1336	a strict consensus sequence	1310:1336	Consistent with other studies, we find that O-GlcNAc sites in T cells lack a strict consensus sequence.
29351928	0	81	theme	T	82:82	arg1	Cells					84:88	Activated Human T Cells	66:88	Activated Human T Cells with Isotope-Targeted Glycoproteomics (Isotag)	66:135	Mapping and Quantification of Over 2000 O-linked Glycopeptides in Activated Human T Cells with Isotope-Targeted Glycoproteomics (Isotag).
29351928	9	82	theme	transcription	1474:1486	arg1	JUNB					1506:1509	JUNB	1506:1509	JUNB	1506:1509	Notably, we observed that the transcription factors c-JUN and JUNB show higher levels of O-GlcNAc glycosylation and higher levels of expression in activated T cells.
29351928	9	82	theme	transcription	1474:1486	arg1	c-JUN					1496:1500	c-JUN	1496:1500	c-JUN	1496:1500	Notably, we observed that the transcription factors c-JUN and JUNB show higher levels of O-GlcNAc glycosylation and higher levels of expression in activated T cells.
29351928	9	82	theme	transcription	1474:1486	arg1	factors					1488:1494	the transcription factors c-JUN and JUNB	1470:1509	the transcription factors c-JUN and JUNB	1470:1509	Notably, we observed that the transcription factors c-JUN and JUNB show higher levels of O-GlcNAc glycosylation and higher levels of expression in activated T cells.
29351928	10	83	gly	glycoproteins	1684:1696	arg1	glycoproteins					1684:1696	O-GlcNAc glycoproteins	1675:1696	O-GlcNAc glycoproteins	1675:1696	Overall, our findings provide a quantitative characterization of O-GlcNAc glycoproteins and their corresponding modification sites in primary human T cells, which will facilitate mechanistic studies into the function of O-GlcNAc in T cell activation.
29351928	10	84	theme	primary	1744:1750	arg1	cells					1760:1764	primary human T cells	1744:1764	primary human T cells	1744:1764	Overall, our findings provide a quantitative characterization of O-GlcNAc glycoproteins and their corresponding modification sites in primary human T cells, which will facilitate mechanistic studies into the function of O-GlcNAc in T cell activation.
29351928	2	85	theme	T	518:518	arg1	activation					525:534	T cell activation	518:534	T cell activation	518:534	Mounting evidence indicates that the modification of proteins by O-linked N-acetylglucosamine (O-GlcNAc), the only mammalian glycan found on nuclear and cytoplasmic proteins, helps regulate T cell activation.
29351928	1	86	from	modifications	157:169	arg1	proteins					181:188	proteins	181:188	proteins	181:188	Post-translational modifications (PTMs) on proteins often function to regulate signaling cascades, with the activation of T cells during an adaptive immune response being a classic example.
29351928	4	87	theme	Isotope	931:937	arg1	Glycoproteomics					948:962	Isotope Targeted Glycoproteomics	931:962	Isotope Targeted Glycoproteomics	931:962	Thus, to advance insight into the role of O-GlcNAc in T cell activation, we performed glycosite mapping studies via direct glycopeptide measurement on resting and activated primary human T cells with a technique termed Isotope Targeted Glycoproteomics.
29351928	9	88	theme	higher	1516:1521	arg1	levels					1523:1528	higher levels	1516:1528	higher levels of O-GlcNAc glycosylation	1516:1554	Notably, we observed that the transcription factors c-JUN and JUNB show higher levels of O-GlcNAc glycosylation and higher levels of expression in activated T cells.
29351928	9	89	from	expression	1577:1586	arg1	cells					1603:1607	activated T cells	1591:1607	activated T cells	1591:1607	Notably, we observed that the transcription factors c-JUN and JUNB show higher levels of O-GlcNAc glycosylation and higher levels of expression in activated T cells.
29351928	2	90	theme	only	438:441	arg1	N-acetylglucosamine					402:420	O-linked N-acetylglucosamine	393:420	O-linked N-acetylglucosamine (O-GlcNAc)	393:431	Mounting evidence indicates that the modification of proteins by O-linked N-acetylglucosamine (O-GlcNAc), the only mammalian glycan found on nuclear and cytoplasmic proteins, helps regulate T cell activation.
29351928	2	90	theme	only	438:441	arg1	glycan					453:458	the only mammalian glycan	434:458	the only mammalian glycan found on nuclear and cytoplasmic proteins	434:500	Mounting evidence indicates that the modification of proteins by O-linked N-acetylglucosamine (O-GlcNAc), the only mammalian glycan found on nuclear and cytoplasmic proteins, helps regulate T cell activation.
29351928	9	91	dep	factors	1488:1494	arg1	JUNB					1506:1509	JUNB	1506:1509	JUNB	1506:1509	Notably, we observed that the transcription factors c-JUN and JUNB show higher levels of O-GlcNAc glycosylation and higher levels of expression in activated T cells.
29351928	9	91	dep	factors	1488:1494	arg1	c-JUN					1496:1500	c-JUN	1496:1500	c-JUN	1496:1500	Notably, we observed that the transcription factors c-JUN and JUNB show higher levels of O-GlcNAc glycosylation and higher levels of expression in activated T cells.
29351928	9	91	dep	factors	1488:1494	arg1	factors					1488:1494	the transcription factors c-JUN and JUNB	1470:1509	the transcription factors c-JUN and JUNB	1470:1509	Notably, we observed that the transcription factors c-JUN and JUNB show higher levels of O-GlcNAc glycosylation and higher levels of expression in activated T cells.
29351928	9	92	from	levels	1567:1572	arg1	cells					1603:1607	activated T cells	1591:1607	activated T cells	1591:1607	Notably, we observed that the transcription factors c-JUN and JUNB show higher levels of O-GlcNAc glycosylation and higher levels of expression in activated T cells.
29351928	7	93	theme	O-GlcNAc	1279:1286	arg1	sites					1288:1292	O-GlcNAc sites	1279:1292	O-GlcNAc sites in T cells	1279:1303	Consistent with other studies, we find that O-GlcNAc sites in T cells lack a strict consensus sequence.
29351928	1	94	theme	immune	287:292	arg1	response					294:301	an adaptive immune response	275:301	an adaptive immune response	275:301	Post-translational modifications (PTMs) on proteins often function to regulate signaling cascades, with the activation of T cells during an adaptive immune response being a classic example.
29351928	9	95	theme	glycosylation	1542:1554	arg1	levels					1523:1528	higher levels	1516:1528	higher levels of O-GlcNAc glycosylation	1516:1554	Notably, we observed that the transcription factors c-JUN and JUNB show higher levels of O-GlcNAc glycosylation and higher levels of expression in activated T cells.
29351928	9	95	theme	glycosylation	1542:1554	arg1	levels					1567:1572	higher levels	1560:1572	higher levels of expression in activated T cells	1560:1607	Notably, we observed that the transcription factors c-JUN and JUNB show higher levels of O-GlcNAc glycosylation and higher levels of expression in activated T cells.
29351928	2	96	link	O-linked	393:400	arg1	O-GlcNAc					423:430	O-GlcNAc	423:430	O-GlcNAc	423:430	Mounting evidence indicates that the modification of proteins by O-linked N-acetylglucosamine (O-GlcNAc), the only mammalian glycan found on nuclear and cytoplasmic proteins, helps regulate T cell activation.
29351928	2	96	link	O-linked	393:400	arg1	glycan					453:458	the only mammalian glycan	434:458	the only mammalian glycan found on nuclear and cytoplasmic proteins	434:500	Mounting evidence indicates that the modification of proteins by O-linked N-acetylglucosamine (O-GlcNAc), the only mammalian glycan found on nuclear and cytoplasmic proteins, helps regulate T cell activation.
29351928	2	96	link	O-linked	393:400	arg1	N-acetylglucosamine					402:420	O-linked N-acetylglucosamine	393:420	O-linked N-acetylglucosamine (O-GlcNAc)	393:431	Mounting evidence indicates that the modification of proteins by O-linked N-acetylglucosamine (O-GlcNAc), the only mammalian glycan found on nuclear and cytoplasmic proteins, helps regulate T cell activation.
29351928	0	97	theme	Glycopeptides	49:61	arg1	Quantification					12:25	Quantification	12:25	Quantification	12:25	Mapping and Quantification of Over 2000 O-linked Glycopeptides in Activated Human T Cells with Isotope-Targeted Glycoproteomics (Isotag).
29351928	0	97	theme	Glycopeptides	49:61	arg1	Mapping					0:6	Mapping	0:6	Mapping	0:6	Mapping and Quantification of Over 2000 O-linked Glycopeptides in Activated Human T Cells with Isotope-Targeted Glycoproteomics (Isotag).
29351928	5	98	theme	intact	1013:1018	arg1	glycopeptides					1029:1041	2219 intact O-linked glycopeptides	1008:1041	2219 intact O-linked glycopeptides across 1045 glycoproteins	1008:1067	This approach led to the identification of 2219 intact O-linked glycopeptides across 1045 glycoproteins.
29351928	6	99	theme	O-GlcNAc	1120:1127	arg1	sites					1129:1133	the identified O-GlcNAc sites	1105:1133	the identified O-GlcNAc sites	1105:1133	A significant proportion (>45%) of the identified O-GlcNAc sites lie near or coincide with a known phosphorylation site, supporting the potential for PTM crosstalk.
29351928	2	100	theme	Mounting	328:335	arg1	evidence					337:344	Mounting evidence	328:344	Mounting evidence	328:344	Mounting evidence indicates that the modification of proteins by O-linked N-acetylglucosamine (O-GlcNAc), the only mammalian glycan found on nuclear and cytoplasmic proteins, helps regulate T cell activation.
29351928	1	101	theme	classic	311:317	arg1	example					319:325	a classic example	309:325	a classic example	309:325	Post-translational modifications (PTMs) on proteins often function to regulate signaling cascades, with the activation of T cells during an adaptive immune response being a classic example.
29351928	1	101	theme	classic	311:317	arg1	activation					246:255	the activation	242:255	the activation of T cells during an adaptive immune response	242:301	Post-translational modifications (PTMs) on proteins often function to regulate signaling cascades, with the activation of T cells during an adaptive immune response being a classic example.
29351928	7	102	from	sites	1288:1292	arg1	cells					1299:1303	T cells	1297:1303	T cells	1297:1303	Consistent with other studies, we find that O-GlcNAc sites in T cells lack a strict consensus sequence.
29351928	7	103	theme	other	1251:1255	arg1	studies					1257:1263	other studies	1251:1263	other studies	1251:1263	Consistent with other studies, we find that O-GlcNAc sites in T cells lack a strict consensus sequence.
29351928	10	104	theme	cell	1844:1847	arg1	activation					1849:1858	T cell activation	1842:1858	T cell activation	1842:1858	Overall, our findings provide a quantitative characterization of O-GlcNAc glycoproteins and their corresponding modification sites in primary human T cells, which will facilitate mechanistic studies into the function of O-GlcNAc in T cell activation.
29351928	8	105	theme	gel	1376:1378	arg1	assays					1386:1391	gel shift assays	1376:1391	gel shift assays	1376:1391	To validate our results, we employed gel shift assays based on conjugating mass tags to O-GlcNAc groups.
29351928	3	106	theme	quantifying	684:694	arg1	sites					705:709	quantifying O-GlcNAc sites	684:709	quantifying O-GlcNAc sites	684:709	Yet, a mechanistic understanding of how O-GlcNAc functions in T cell activation remains elusive, partly because of the difficulties in mapping and quantifying O-GlcNAc sites.
29351928	4	107	theme	cell	768:771	arg1	activation					773:782	T cell activation	766:782	T cell activation	766:782	Thus, to advance insight into the role of O-GlcNAc in T cell activation, we performed glycosite mapping studies via direct glycopeptide measurement on resting and activated primary human T cells with a technique termed Isotope Targeted Glycoproteomics.
29851350	9	0	theme	large-scale	1882:1892	arg1	research					1912:1919	large-scale N-glycoproteomics research	1882:1919	large-scale N-glycoproteomics research	1882:1919	All the results have demonstrated that MMP has great potential in large-scale N-glycoproteomics research.
29851350	2	1	gly	nonglycopeptides	304:319	arg2	nonglycopeptides					304:319	nonglycopeptides	304:319	nonglycopeptides	304:319	Due to the low contents of glycopeptides compared with nonglycopeptides and the microheterogeneity of glycosylation sites, highly selective enrichment methods for the purification of glycopeptides are required for the comprehensive characterization of glycoproteomics.
29851350	2	1	gly	nonglycopeptides	304:319	arg1	sites					365:369	glycosylation sites	351:369	glycosylation sites	351:369	Due to the low contents of glycopeptides compared with nonglycopeptides and the microheterogeneity of glycosylation sites, highly selective enrichment methods for the purification of glycopeptides are required for the comprehensive characterization of glycoproteomics.
29851350	2	2	theme	glycopeptides	276:288	arg1	contents					264:271	the low contents	256:271	the low contents of glycopeptides compared with nonglycopeptides and the microheterogeneity of glycosylation sites	256:369	Due to the low contents of glycopeptides compared with nonglycopeptides and the microheterogeneity of glycosylation sites, highly selective enrichment methods for the purification of glycopeptides are required for the comprehensive characterization of glycoproteomics.
29851350	5	3	from	groups	1074:1079	arg1	MMP					1088:1090	MMP	1088:1090	MMP	1088:1090	By taking advantage of the interaction between hydrophilic groups on the MMP and glycan components on the glycopeptides, the acquired MMP was utilized to the selective capture of N-glycopeptides (human IgG or HRP tryptic digests/BSA proteins = 1:50), good recovery yield (70.18-97.23%), superior binding capacity (400 mg g-1), and excellent reproducibility.
29851350	5	3	from	groups	1074:1079	arg1	components					1103:1112	glycan components	1096:1112	glycan components	1096:1112	By taking advantage of the interaction between hydrophilic groups on the MMP and glycan components on the glycopeptides, the acquired MMP was utilized to the selective capture of N-glycopeptides (human IgG or HRP tryptic digests/BSA proteins = 1:50), good recovery yield (70.18-97.23%), superior binding capacity (400 mg g-1), and excellent reproducibility.
29851350	7	4	gly	N-glycopeptides	1570:1584	arg2	N-glycopeptides					1570:1584	15 unique N-glycopeptides	1560:1584	15 unique N-glycopeptides	1560:1584	A total of 15 unique N-glycopeptides were identified from an ultralow sample volume (0.025 μL) of human serum.
29851350	3	5	theme	aqueous	737:743	arg1	system					745:750	a resorcinol/formaldehyde monomer aqueous system	703:750	a resorcinol/formaldehyde monomer aqueous system	703:750	In this work, a type of magnetic mesoporous phenolic resin (MMP) was prepared using branched polyethylenimine (PEI) as a cross-linker from a homogeneous magnetic Fe3O4@SiO2 solution in a resorcinol/formaldehyde monomer aqueous system via an in situ emulsion polymerization procedure.
29851350	3	6	theme	resin	571:575	arg1	type					534:537	a type	532:537	a type of magnetic mesoporous phenolic resin (MMP)	532:581	In this work, a type of magnetic mesoporous phenolic resin (MMP) was prepared using branched polyethylenimine (PEI) as a cross-linker from a homogeneous magnetic Fe3O4@SiO2 solution in a resorcinol/formaldehyde monomer aqueous system via an in situ emulsion polymerization procedure.
29851350	1	7	theme	biological	167:176	arg1	processes					178:186	biological processes	167:186	biological processes	167:186	Protein glycosylation is a significant participant in a mass of biological processes, which is a pivotal protein post-translational modification.
29851350	3	8	from	solution	691:698	arg1	system					745:750	a resorcinol/formaldehyde monomer aqueous system	703:750	a resorcinol/formaldehyde monomer aqueous system	703:750	In this work, a type of magnetic mesoporous phenolic resin (MMP) was prepared using branched polyethylenimine (PEI) as a cross-linker from a homogeneous magnetic Fe3O4@SiO2 solution in a resorcinol/formaldehyde monomer aqueous system via an in situ emulsion polymerization procedure.
29851350	3	8	from	solution	691:698	arg1	polyethylenimine					611:626	branched polyethylenimine	602:626	branched polyethylenimine (PEI)	602:632	In this work, a type of magnetic mesoporous phenolic resin (MMP) was prepared using branched polyethylenimine (PEI) as a cross-linker from a homogeneous magnetic Fe3O4@SiO2 solution in a resorcinol/formaldehyde monomer aqueous system via an in situ emulsion polymerization procedure.
29851350	3	8	from	solution	691:698	arg1	cross-linker					639:650	a cross-linker	637:650	a cross-linker from a homogeneous magnetic Fe3O4@SiO2 solution in a resorcinol/formaldehyde monomer aqueous system via an in situ emulsion polymerization procedure	637:799	In this work, a type of magnetic mesoporous phenolic resin (MMP) was prepared using branched polyethylenimine (PEI) as a cross-linker from a homogeneous magnetic Fe3O4@SiO2 solution in a resorcinol/formaldehyde monomer aqueous system via an in situ emulsion polymerization procedure.
29851350	4	9	theme	nitrogen-containing	887:905	arg1	functionality					907:919	nitrogen-containing functionality	887:919	nitrogen-containing functionality	887:919	The results showed that MMP exhibited good biocompatibility, a mesoporous structure, nitrogen-containing functionality, excellent hydrophilicity, and solvent resistance by using multiple characterization methods.
29851350	3	10	theme	emulsion	767:774	arg1	procedure					791:799	an in situ emulsion polymerization procedure	756:799	an in situ emulsion polymerization procedure	756:799	In this work, a type of magnetic mesoporous phenolic resin (MMP) was prepared using branched polyethylenimine (PEI) as a cross-linker from a homogeneous magnetic Fe3O4@SiO2 solution in a resorcinol/formaldehyde monomer aqueous system via an in situ emulsion polymerization procedure.
29851350	3	11	theme	in	759:760	arg1	procedure					791:799	an in situ emulsion polymerization procedure	756:799	an in situ emulsion polymerization procedure	756:799	In this work, a type of magnetic mesoporous phenolic resin (MMP) was prepared using branched polyethylenimine (PEI) as a cross-linker from a homogeneous magnetic Fe3O4@SiO2 solution in a resorcinol/formaldehyde monomer aqueous system via an in situ emulsion polymerization procedure.
29851350	6	12	theme	serum	1542:1546	arg1	digests					1525:1531	tryptic digests	1517:1531	tryptic digests of human serum	1517:1546	Based on the outstanding performance in standard glycoproteins tryptic digests enrichment, MMP was further used to capture N-glycopeptides from tryptic digests of human serum.
29851350	7	13	theme	human	1647:1651	arg1	serum					1653:1657	human serum	1647:1657	human serum	1647:1657	A total of 15 unique N-glycopeptides were identified from an ultralow sample volume (0.025 μL) of human serum.
29851350	4	14	theme	characterization	989:1004	arg1	methods					1006:1012	multiple characterization methods	980:1012	multiple characterization methods	980:1012	The results showed that MMP exhibited good biocompatibility, a mesoporous structure, nitrogen-containing functionality, excellent hydrophilicity, and solvent resistance by using multiple characterization methods.
29851350	9	15	theme	N-glycoproteomics	1894:1910	arg1	research					1912:1919	large-scale N-glycoproteomics research	1882:1919	large-scale N-glycoproteomics research	1882:1919	All the results have demonstrated that MMP has great potential in large-scale N-glycoproteomics research.
29851350	6	16	used	used	1480:1483	arg2	MMP					1464:1466	MMP	1464:1466	MMP	1464:1466	Based on the outstanding performance in standard glycoproteins tryptic digests enrichment, MMP was further used to capture N-glycopeptides from tryptic digests of human serum.
29851350	8	17	dep	analyses	1806:1813	arg1	replicate					1796:1804	replicate	1796:1804	replicate	1796:1804	Overall, we identified 356 unique N-glycopeptides corresponding to 119 glycoproteins from human serum (0.35 μL) in the overlap of three replicate analyses.
29851350	5	18	theme	acquired	1140:1147	arg1	MMP					1149:1151	the acquired MMP	1136:1151	the acquired MMP	1136:1151	By taking advantage of the interaction between hydrophilic groups on the MMP and glycan components on the glycopeptides, the acquired MMP was utilized to the selective capture of N-glycopeptides (human IgG or HRP tryptic digests/BSA proteins = 1:50), good recovery yield (70.18-97.23%), superior binding capacity (400 mg g-1), and excellent reproducibility.
29851350	1	19	theme	pivotal	200:206	arg1	modification					235:246	a pivotal protein post-translational modification	198:246	a pivotal protein post-translational modification	198:246	Protein glycosylation is a significant participant in a mass of biological processes, which is a pivotal protein post-translational modification.
29851350	1	19	theme	pivotal	200:206	arg1	glycosylation					111:123	Protein glycosylation	103:123	Protein glycosylation	103:123	Protein glycosylation is a significant participant in a mass of biological processes, which is a pivotal protein post-translational modification.
29851350	1	19	theme	pivotal	200:206	arg1	participant					142:152	a significant participant	128:152	a significant participant	128:152	Protein glycosylation is a significant participant in a mass of biological processes, which is a pivotal protein post-translational modification.
29851350	5	20	theme	binding	1311:1317	arg1	g-1					1336:1338	400 mg g-1	1329:1338	400 mg g-1	1329:1338	By taking advantage of the interaction between hydrophilic groups on the MMP and glycan components on the glycopeptides, the acquired MMP was utilized to the selective capture of N-glycopeptides (human IgG or HRP tryptic digests/BSA proteins = 1:50), good recovery yield (70.18-97.23%), superior binding capacity (400 mg g-1), and excellent reproducibility.
29851350	5	20	theme	binding	1311:1317	arg1	capacity					1319:1326	superior binding capacity	1302:1326	superior binding capacity (400 mg g-1)	1302:1339	By taking advantage of the interaction between hydrophilic groups on the MMP and glycan components on the glycopeptides, the acquired MMP was utilized to the selective capture of N-glycopeptides (human IgG or HRP tryptic digests/BSA proteins = 1:50), good recovery yield (70.18-97.23%), superior binding capacity (400 mg g-1), and excellent reproducibility.
29851350	1	21	theme	significant	130:140	arg1	modification					235:246	a pivotal protein post-translational modification	198:246	a pivotal protein post-translational modification	198:246	Protein glycosylation is a significant participant in a mass of biological processes, which is a pivotal protein post-translational modification.
29851350	1	21	theme	significant	130:140	arg1	glycosylation					111:123	Protein glycosylation	103:123	Protein glycosylation	103:123	Protein glycosylation is a significant participant in a mass of biological processes, which is a pivotal protein post-translational modification.
29851350	1	21	theme	significant	130:140	arg1	participant					142:152	a significant participant	128:152	a significant participant	128:152	Protein glycosylation is a significant participant in a mass of biological processes, which is a pivotal protein post-translational modification.
29851350	5	22	used	utilized	1157:1164	arg2	MMP					1149:1151	the acquired MMP	1136:1151	the acquired MMP	1136:1151	By taking advantage of the interaction between hydrophilic groups on the MMP and glycan components on the glycopeptides, the acquired MMP was utilized to the selective capture of N-glycopeptides (human IgG or HRP tryptic digests/BSA proteins = 1:50), good recovery yield (70.18-97.23%), superior binding capacity (400 mg g-1), and excellent reproducibility.
29851350	5	23	theme	selective	1173:1181	arg1	capture					1183:1189	the selective capture	1169:1189	the selective capture of N-glycopeptides (human IgG or HRP tryptic digests/BSA proteins = 1:50), good recovery yield (70.18-97.23%), superior binding capacity (400 mg g-1), and excellent reproducibility	1169:1370	By taking advantage of the interaction between hydrophilic groups on the MMP and glycan components on the glycopeptides, the acquired MMP was utilized to the selective capture of N-glycopeptides (human IgG or HRP tryptic digests/BSA proteins = 1:50), good recovery yield (70.18-97.23%), superior binding capacity (400 mg g-1), and excellent reproducibility.
29851350	7	24	theme	ultralow	1610:1617	arg1	μL					1640:1641	0.025 μL	1634:1641	0.025 μL	1634:1641	A total of 15 unique N-glycopeptides were identified from an ultralow sample volume (0.025 μL) of human serum.
29851350	7	24	theme	ultralow	1610:1617	arg1	volume					1626:1631	an ultralow sample volume	1607:1631	an ultralow sample volume (0.025 μL) of human serum	1607:1657	A total of 15 unique N-glycopeptides were identified from an ultralow sample volume (0.025 μL) of human serum.
29851350	0	25	theme	Selective	64:72	arg1	Enrichment					74:83	the Selective Enrichment	60:83	the Selective Enrichment of Glycopeptides	60:100	In Situ Synthesis of Magnetic Mesoporous Phenolic Resin for the Selective Enrichment of Glycopeptides.
29851350	1	26	from	participant	142:152	arg1	mass					159:162	a mass	157:162	a mass of biological processes	157:186	Protein glycosylation is a significant participant in a mass of biological processes, which is a pivotal protein post-translational modification.
29851350	5	27	theme	interaction	1042:1052	arg1	advantage					1025:1033	advantage	1025:1033	advantage of the interaction between hydrophilic groups on the MMP and glycan components on the glycopeptides	1025:1133	By taking advantage of the interaction between hydrophilic groups on the MMP and glycan components on the glycopeptides, the acquired MMP was utilized to the selective capture of N-glycopeptides (human IgG or HRP tryptic digests/BSA proteins = 1:50), good recovery yield (70.18-97.23%), superior binding capacity (400 mg g-1), and excellent reproducibility.
29851350	5	28	theme	reproducibility	1356:1370	arg1	capture					1183:1189	the selective capture	1169:1189	the selective capture of N-glycopeptides (human IgG or HRP tryptic digests/BSA proteins = 1:50), good recovery yield (70.18-97.23%), superior binding capacity (400 mg g-1), and excellent reproducibility	1169:1370	By taking advantage of the interaction between hydrophilic groups on the MMP and glycan components on the glycopeptides, the acquired MMP was utilized to the selective capture of N-glycopeptides (human IgG or HRP tryptic digests/BSA proteins = 1:50), good recovery yield (70.18-97.23%), superior binding capacity (400 mg g-1), and excellent reproducibility.
29851350	0	29	theme	In	0:1	arg1	Synthesis					8:16	In Situ Synthesis	0:16	In Situ Synthesis of Magnetic Mesoporous Phenolic Resin for the Selective Enrichment of Glycopeptides.	0:101	In Situ Synthesis of Magnetic Mesoporous Phenolic Resin for the Selective Enrichment of Glycopeptides.
29851350	5	30	dep	N-glycopeptides	1194:1208	arg1	IgG					1217:1219	human IgG	1211:1219	human IgG	1211:1219	By taking advantage of the interaction between hydrophilic groups on the MMP and glycan components on the glycopeptides, the acquired MMP was utilized to the selective capture of N-glycopeptides (human IgG or HRP tryptic digests/BSA proteins = 1:50), good recovery yield (70.18-97.23%), superior binding capacity (400 mg g-1), and excellent reproducibility.
29851350	5	30	dep	N-glycopeptides	1194:1208	arg1	HRP					1224:1226	HRP	1224:1226	HRP	1224:1226	By taking advantage of the interaction between hydrophilic groups on the MMP and glycan components on the glycopeptides, the acquired MMP was utilized to the selective capture of N-glycopeptides (human IgG or HRP tryptic digests/BSA proteins = 1:50), good recovery yield (70.18-97.23%), superior binding capacity (400 mg g-1), and excellent reproducibility.
29851350	5	31	theme	mg	1333:1334	arg1	g-1					1336:1338	400 mg g-1	1329:1338	400 mg g-1	1329:1338	By taking advantage of the interaction between hydrophilic groups on the MMP and glycan components on the glycopeptides, the acquired MMP was utilized to the selective capture of N-glycopeptides (human IgG or HRP tryptic digests/BSA proteins = 1:50), good recovery yield (70.18-97.23%), superior binding capacity (400 mg g-1), and excellent reproducibility.
29851350	5	31	theme	mg	1333:1334	arg1	capacity					1319:1326	superior binding capacity	1302:1326	superior binding capacity (400 mg g-1)	1302:1339	By taking advantage of the interaction between hydrophilic groups on the MMP and glycan components on the glycopeptides, the acquired MMP was utilized to the selective capture of N-glycopeptides (human IgG or HRP tryptic digests/BSA proteins = 1:50), good recovery yield (70.18-97.23%), superior binding capacity (400 mg g-1), and excellent reproducibility.
29851350	5	32	theme	digests/BSA	1236:1246	arg1	proteins					1248:1255	tryptic digests/BSA proteins	1228:1255	tryptic digests/BSA proteins	1228:1255	By taking advantage of the interaction between hydrophilic groups on the MMP and glycan components on the glycopeptides, the acquired MMP was utilized to the selective capture of N-glycopeptides (human IgG or HRP tryptic digests/BSA proteins = 1:50), good recovery yield (70.18-97.23%), superior binding capacity (400 mg g-1), and excellent reproducibility.
29851350	1	33	theme	Protein	103:109	arg1	modification					235:246	a pivotal protein post-translational modification	198:246	a pivotal protein post-translational modification	198:246	Protein glycosylation is a significant participant in a mass of biological processes, which is a pivotal protein post-translational modification.
29851350	1	33	theme	Protein	103:109	arg1	glycosylation					111:123	Protein glycosylation	103:123	Protein glycosylation	103:123	Protein glycosylation is a significant participant in a mass of biological processes, which is a pivotal protein post-translational modification.
29851350	1	33	theme	Protein	103:109	arg1	participant					142:152	a significant participant	128:152	a significant participant	128:152	Protein glycosylation is a significant participant in a mass of biological processes, which is a pivotal protein post-translational modification.
29851350	8	34	gly	glycoproteins	1731:1743	arg1	glycoproteins					1731:1743	119 glycoproteins	1727:1743	119 glycoproteins from human serum (0.35 μL)	1727:1770	Overall, we identified 356 unique N-glycopeptides corresponding to 119 glycoproteins from human serum (0.35 μL) in the overlap of three replicate analyses.
29851350	3	35	theme	magnetic	671:678	arg1	solution					691:698	a homogeneous magnetic Fe3O4@SiO2 solution	657:698	a homogeneous magnetic Fe3O4@SiO2 solution in a resorcinol/formaldehyde monomer aqueous system via an in situ emulsion polymerization procedure	657:799	In this work, a type of magnetic mesoporous phenolic resin (MMP) was prepared using branched polyethylenimine (PEI) as a cross-linker from a homogeneous magnetic Fe3O4@SiO2 solution in a resorcinol/formaldehyde monomer aqueous system via an in situ emulsion polymerization procedure.
29851350	2	36	theme	glycopeptides	432:444	arg1	purification					416:427	the purification	412:427	the purification of glycopeptides	412:444	Due to the low contents of glycopeptides compared with nonglycopeptides and the microheterogeneity of glycosylation sites, highly selective enrichment methods for the purification of glycopeptides are required for the comprehensive characterization of glycoproteomics.
29851350	0	37	theme	Magnetic	21:28	arg1	Resin					50:54	Magnetic Mesoporous Phenolic Resin	21:54	Magnetic Mesoporous Phenolic Resin	21:54	In Situ Synthesis of Magnetic Mesoporous Phenolic Resin for the Selective Enrichment of Glycopeptides.
29851350	6	38	dep	digests	1444:1450	arg1	enrichment					1452:1461	enrichment	1452:1461	standard glycoproteins tryptic digests enrichment	1413:1461	Based on the outstanding performance in standard glycoproteins tryptic digests enrichment, MMP was further used to capture N-glycopeptides from tryptic digests of human serum.
29851350	2	39	theme	glycoproteomics	501:515	arg1	characterization					481:496	the comprehensive characterization	463:496	the comprehensive characterization of glycoproteomics	463:515	Due to the low contents of glycopeptides compared with nonglycopeptides and the microheterogeneity of glycosylation sites, highly selective enrichment methods for the purification of glycopeptides are required for the comprehensive characterization of glycoproteomics.
29851350	2	40	gly	glycosylation	351:363	arg2	sites					365:369	glycosylation sites	351:369	glycosylation sites	351:369	Due to the low contents of glycopeptides compared with nonglycopeptides and the microheterogeneity of glycosylation sites, highly selective enrichment methods for the purification of glycopeptides are required for the comprehensive characterization of glycoproteomics.
29851350	2	41	theme	sites	365:369	arg1	nonglycopeptides					304:319	nonglycopeptides	304:319	nonglycopeptides	304:319	Due to the low contents of glycopeptides compared with nonglycopeptides and the microheterogeneity of glycosylation sites, highly selective enrichment methods for the purification of glycopeptides are required for the comprehensive characterization of glycoproteomics.
29851350	2	41	theme	sites	365:369	arg1	microheterogeneity					329:346	the microheterogeneity	325:346	the microheterogeneity of glycosylation sites	325:369	Due to the low contents of glycopeptides compared with nonglycopeptides and the microheterogeneity of glycosylation sites, highly selective enrichment methods for the purification of glycopeptides are required for the comprehensive characterization of glycoproteomics.
29851350	0	42	theme	Phenolic	41:48	arg1	Resin					50:54	Magnetic Mesoporous Phenolic Resin	21:54	Magnetic Mesoporous Phenolic Resin	21:54	In Situ Synthesis of Magnetic Mesoporous Phenolic Resin for the Selective Enrichment of Glycopeptides.
29851350	3	43	dep	in	759:760	arg1	situ					762:765	situ	762:765	situ	762:765	In this work, a type of magnetic mesoporous phenolic resin (MMP) was prepared using branched polyethylenimine (PEI) as a cross-linker from a homogeneous magnetic Fe3O4@SiO2 solution in a resorcinol/formaldehyde monomer aqueous system via an in situ emulsion polymerization procedure.
29851350	5	44	theme	glycan	1096:1101	arg1	components					1103:1112	glycan components	1096:1112	glycan components	1096:1112	By taking advantage of the interaction between hydrophilic groups on the MMP and glycan components on the glycopeptides, the acquired MMP was utilized to the selective capture of N-glycopeptides (human IgG or HRP tryptic digests/BSA proteins = 1:50), good recovery yield (70.18-97.23%), superior binding capacity (400 mg g-1), and excellent reproducibility.
29851350	2	45	gly	microheterogeneity	329:346	arg1	sites					365:369	glycosylation sites	351:369	glycosylation sites	351:369	Due to the low contents of glycopeptides compared with nonglycopeptides and the microheterogeneity of glycosylation sites, highly selective enrichment methods for the purification of glycopeptides are required for the comprehensive characterization of glycoproteomics.
29851350	5	46	theme	good	1266:1269	arg1	yield					1280:1284	good recovery yield	1266:1284	good recovery yield (70.18-97.23%)	1266:1299	By taking advantage of the interaction between hydrophilic groups on the MMP and glycan components on the glycopeptides, the acquired MMP was utilized to the selective capture of N-glycopeptides (human IgG or HRP tryptic digests/BSA proteins = 1:50), good recovery yield (70.18-97.23%), superior binding capacity (400 mg g-1), and excellent reproducibility.
29851350	5	46	theme	good	1266:1269	arg1	%					1298:1298	70.18-97.23%	1287:1298	70.18-97.23%	1287:1298	By taking advantage of the interaction between hydrophilic groups on the MMP and glycan components on the glycopeptides, the acquired MMP was utilized to the selective capture of N-glycopeptides (human IgG or HRP tryptic digests/BSA proteins = 1:50), good recovery yield (70.18-97.23%), superior binding capacity (400 mg g-1), and excellent reproducibility.
29851350	5	47	theme	hydrophilic	1062:1072	arg1	groups					1074:1079	hydrophilic groups	1062:1079	hydrophilic groups on the MMP and glycan components on the glycopeptides	1062:1133	By taking advantage of the interaction between hydrophilic groups on the MMP and glycan components on the glycopeptides, the acquired MMP was utilized to the selective capture of N-glycopeptides (human IgG or HRP tryptic digests/BSA proteins = 1:50), good recovery yield (70.18-97.23%), superior binding capacity (400 mg g-1), and excellent reproducibility.
29851350	9	48	theme	great	1863:1867	arg1	potential					1869:1877	great potential	1863:1877	great potential	1863:1877	All the results have demonstrated that MMP has great potential in large-scale N-glycoproteomics research.
29851350	3	49	theme	@	685:685	arg1	solution					691:698	a homogeneous magnetic Fe3O4@SiO2 solution	657:698	a homogeneous magnetic Fe3O4@SiO2 solution in a resorcinol/formaldehyde monomer aqueous system via an in situ emulsion polymerization procedure	657:799	In this work, a type of magnetic mesoporous phenolic resin (MMP) was prepared using branched polyethylenimine (PEI) as a cross-linker from a homogeneous magnetic Fe3O4@SiO2 solution in a resorcinol/formaldehyde monomer aqueous system via an in situ emulsion polymerization procedure.
29851350	8	50	from	serum	1756:1760	arg1	glycoproteins					1731:1743	119 glycoproteins	1727:1743	119 glycoproteins from human serum (0.35 μL)	1727:1770	Overall, we identified 356 unique N-glycopeptides corresponding to 119 glycoproteins from human serum (0.35 μL) in the overlap of three replicate analyses.
29851350	3	51	theme	magnetic	542:549	arg1	MMP					578:580	MMP	578:580	MMP	578:580	In this work, a type of magnetic mesoporous phenolic resin (MMP) was prepared using branched polyethylenimine (PEI) as a cross-linker from a homogeneous magnetic Fe3O4@SiO2 solution in a resorcinol/formaldehyde monomer aqueous system via an in situ emulsion polymerization procedure.
29851350	3	51	theme	magnetic	542:549	arg1	resin					571:575	magnetic mesoporous phenolic resin	542:575	magnetic mesoporous phenolic resin (MMP)	542:581	In this work, a type of magnetic mesoporous phenolic resin (MMP) was prepared using branched polyethylenimine (PEI) as a cross-linker from a homogeneous magnetic Fe3O4@SiO2 solution in a resorcinol/formaldehyde monomer aqueous system via an in situ emulsion polymerization procedure.
29851350	5	52	theme	yield	1280:1284	arg1	capture					1183:1189	the selective capture	1169:1189	the selective capture of N-glycopeptides (human IgG or HRP tryptic digests/BSA proteins = 1:50), good recovery yield (70.18-97.23%), superior binding capacity (400 mg g-1), and excellent reproducibility	1169:1370	By taking advantage of the interaction between hydrophilic groups on the MMP and glycan components on the glycopeptides, the acquired MMP was utilized to the selective capture of N-glycopeptides (human IgG or HRP tryptic digests/BSA proteins = 1:50), good recovery yield (70.18-97.23%), superior binding capacity (400 mg g-1), and excellent reproducibility.
29851350	6	53	theme	tryptic	1436:1442	arg1	digests					1444:1450	standard glycoproteins tryptic digests enrichment	1413:1461	standard glycoproteins tryptic digests enrichment	1413:1461	Based on the outstanding performance in standard glycoproteins tryptic digests enrichment, MMP was further used to capture N-glycopeptides from tryptic digests of human serum.
29851350	0	54	dep	In	0:1	arg1	Situ					3:6	Situ	3:6	Situ	3:6	In Situ Synthesis of Magnetic Mesoporous Phenolic Resin for the Selective Enrichment of Glycopeptides.
29851350	8	55	dep	the	1775:1777	arg1	overlap					1779:1785	overlap	1779:1785	overlap	1779:1785	Overall, we identified 356 unique N-glycopeptides corresponding to 119 glycoproteins from human serum (0.35 μL) in the overlap of three replicate analyses.
29851350	3	56	theme	monomer	729:735	arg1	system					745:750	a resorcinol/formaldehyde monomer aqueous system	703:750	a resorcinol/formaldehyde monomer aqueous system	703:750	In this work, a type of magnetic mesoporous phenolic resin (MMP) was prepared using branched polyethylenimine (PEI) as a cross-linker from a homogeneous magnetic Fe3O4@SiO2 solution in a resorcinol/formaldehyde monomer aqueous system via an in situ emulsion polymerization procedure.
29851350	5	57	theme	=	1257:1257	arg1	IgG					1217:1219	human IgG	1211:1219	human IgG	1211:1219	By taking advantage of the interaction between hydrophilic groups on the MMP and glycan components on the glycopeptides, the acquired MMP was utilized to the selective capture of N-glycopeptides (human IgG or HRP tryptic digests/BSA proteins = 1:50), good recovery yield (70.18-97.23%), superior binding capacity (400 mg g-1), and excellent reproducibility.
29851350	8	58	gly	N-glycopeptides	1694:1708	arg2	N-glycopeptides					1694:1708	356 unique N-glycopeptides	1683:1708	356 unique N-glycopeptides corresponding to 119 glycoproteins from human serum (0.35 μL) in the overlap of three replicate analyses	1683:1813	Overall, we identified 356 unique N-glycopeptides corresponding to 119 glycoproteins from human serum (0.35 μL) in the overlap of three replicate analyses.
29851350	5	59	gly	glycopeptides	1121:1133	arg2	glycopeptides					1121:1133	the glycopeptides	1117:1133	the glycopeptides	1117:1133	By taking advantage of the interaction between hydrophilic groups on the MMP and glycan components on the glycopeptides, the acquired MMP was utilized to the selective capture of N-glycopeptides (human IgG or HRP tryptic digests/BSA proteins = 1:50), good recovery yield (70.18-97.23%), superior binding capacity (400 mg g-1), and excellent reproducibility.
29851350	2	60	gly	glycopeptides	276:288	arg2	glycopeptides					276:288	glycopeptides	276:288	glycopeptides compared with nonglycopeptides and the microheterogeneity of glycosylation sites	276:369	Due to the low contents of glycopeptides compared with nonglycopeptides and the microheterogeneity of glycosylation sites, highly selective enrichment methods for the purification of glycopeptides are required for the comprehensive characterization of glycoproteomics.
29851350	4	61	theme	excellent	922:930	arg1	hydrophilicity					932:945	excellent hydrophilicity	922:945	excellent hydrophilicity	922:945	The results showed that MMP exhibited good biocompatibility, a mesoporous structure, nitrogen-containing functionality, excellent hydrophilicity, and solvent resistance by using multiple characterization methods.
29851350	3	62	theme	phenolic	562:569	arg1	MMP					578:580	MMP	578:580	MMP	578:580	In this work, a type of magnetic mesoporous phenolic resin (MMP) was prepared using branched polyethylenimine (PEI) as a cross-linker from a homogeneous magnetic Fe3O4@SiO2 solution in a resorcinol/formaldehyde monomer aqueous system via an in situ emulsion polymerization procedure.
29851350	3	62	theme	phenolic	562:569	arg1	resin					571:575	magnetic mesoporous phenolic resin	542:575	magnetic mesoporous phenolic resin (MMP)	542:581	In this work, a type of magnetic mesoporous phenolic resin (MMP) was prepared using branched polyethylenimine (PEI) as a cross-linker from a homogeneous magnetic Fe3O4@SiO2 solution in a resorcinol/formaldehyde monomer aqueous system via an in situ emulsion polymerization procedure.
29851350	6	63	theme	glycoproteins	1422:1434	arg1	digests					1444:1450	standard glycoproteins tryptic digests enrichment	1413:1461	standard glycoproteins tryptic digests enrichment	1413:1461	Based on the outstanding performance in standard glycoproteins tryptic digests enrichment, MMP was further used to capture N-glycopeptides from tryptic digests of human serum.
29851350	4	64	theme	mesoporous	865:874	arg1	structure					876:884	a mesoporous structure	863:884	a mesoporous structure	863:884	The results showed that MMP exhibited good biocompatibility, a mesoporous structure, nitrogen-containing functionality, excellent hydrophilicity, and solvent resistance by using multiple characterization methods.
29851350	5	65	gly	N-glycopeptides	1194:1208	arg2	N-glycopeptides					1194:1208	N-glycopeptides	1194:1208	N-glycopeptides (human IgG or HRP tryptic digests/BSA proteins = 1:50)	1194:1263	By taking advantage of the interaction between hydrophilic groups on the MMP and glycan components on the glycopeptides, the acquired MMP was utilized to the selective capture of N-glycopeptides (human IgG or HRP tryptic digests/BSA proteins = 1:50), good recovery yield (70.18-97.23%), superior binding capacity (400 mg g-1), and excellent reproducibility.
29851350	6	66	theme	outstanding	1386:1396	arg1	performance					1398:1408	the outstanding performance	1382:1408	the outstanding performance in standard glycoproteins tryptic digests enrichment	1382:1461	Based on the outstanding performance in standard glycoproteins tryptic digests enrichment, MMP was further used to capture N-glycopeptides from tryptic digests of human serum.
29851350	1	67	theme	processes	178:186	arg1	mass					159:162	a mass	157:162	a mass of biological processes	157:186	Protein glycosylation is a significant participant in a mass of biological processes, which is a pivotal protein post-translational modification.
29851350	7	68	theme	serum	1653:1657	arg1	μL					1640:1641	0.025 μL	1634:1641	0.025 μL	1634:1641	A total of 15 unique N-glycopeptides were identified from an ultralow sample volume (0.025 μL) of human serum.
29851350	7	68	theme	serum	1653:1657	arg1	volume					1626:1631	an ultralow sample volume	1607:1631	an ultralow sample volume (0.025 μL) of human serum	1607:1657	A total of 15 unique N-glycopeptides were identified from an ultralow sample volume (0.025 μL) of human serum.
29851350	5	69	dep	MMP	1088:1090	arg1	the					1084:1086	the	1084:1086	the	1084:1086	By taking advantage of the interaction between hydrophilic groups on the MMP and glycan components on the glycopeptides, the acquired MMP was utilized to the selective capture of N-glycopeptides (human IgG or HRP tryptic digests/BSA proteins = 1:50), good recovery yield (70.18-97.23%), superior binding capacity (400 mg g-1), and excellent reproducibility.
29851350	3	70	theme	polymerization	776:789	arg1	procedure					791:799	an in situ emulsion polymerization procedure	756:799	an in situ emulsion polymerization procedure	756:799	In this work, a type of magnetic mesoporous phenolic resin (MMP) was prepared using branched polyethylenimine (PEI) as a cross-linker from a homogeneous magnetic Fe3O4@SiO2 solution in a resorcinol/formaldehyde monomer aqueous system via an in situ emulsion polymerization procedure.
29851350	8	71	theme	human	1750:1754	arg1	serum					1756:1760	human serum	1750:1760	human serum (0.35 μL)	1750:1770	Overall, we identified 356 unique N-glycopeptides corresponding to 119 glycoproteins from human serum (0.35 μL) in the overlap of three replicate analyses.
29851350	8	71	theme	human	1750:1754	arg1	μL					1768:1769	0.35 μL	1763:1769	0.35 μL	1763:1769	Overall, we identified 356 unique N-glycopeptides corresponding to 119 glycoproteins from human serum (0.35 μL) in the overlap of three replicate analyses.
29851350	5	72	from	MMP	1088:1090	arg1	glycopeptides					1121:1133	the glycopeptides	1117:1133	the glycopeptides	1117:1133	By taking advantage of the interaction between hydrophilic groups on the MMP and glycan components on the glycopeptides, the acquired MMP was utilized to the selective capture of N-glycopeptides (human IgG or HRP tryptic digests/BSA proteins = 1:50), good recovery yield (70.18-97.23%), superior binding capacity (400 mg g-1), and excellent reproducibility.
29851350	4	73	theme	multiple	980:987	arg1	methods					1006:1012	multiple characterization methods	980:1012	multiple characterization methods	980:1012	The results showed that MMP exhibited good biocompatibility, a mesoporous structure, nitrogen-containing functionality, excellent hydrophilicity, and solvent resistance by using multiple characterization methods.
29851350	3	74	theme	branched	602:609	arg1	PEI					629:631	PEI	629:631	PEI	629:631	In this work, a type of magnetic mesoporous phenolic resin (MMP) was prepared using branched polyethylenimine (PEI) as a cross-linker from a homogeneous magnetic Fe3O4@SiO2 solution in a resorcinol/formaldehyde monomer aqueous system via an in situ emulsion polymerization procedure.
29851350	3	74	theme	branched	602:609	arg1	polyethylenimine					611:626	branched polyethylenimine	602:626	branched polyethylenimine (PEI)	602:632	In this work, a type of magnetic mesoporous phenolic resin (MMP) was prepared using branched polyethylenimine (PEI) as a cross-linker from a homogeneous magnetic Fe3O4@SiO2 solution in a resorcinol/formaldehyde monomer aqueous system via an in situ emulsion polymerization procedure.
29851350	3	74	theme	branched	602:609	arg1	cross-linker					639:650	a cross-linker	637:650	a cross-linker from a homogeneous magnetic Fe3O4@SiO2 solution in a resorcinol/formaldehyde monomer aqueous system via an in situ emulsion polymerization procedure	637:799	In this work, a type of magnetic mesoporous phenolic resin (MMP) was prepared using branched polyethylenimine (PEI) as a cross-linker from a homogeneous magnetic Fe3O4@SiO2 solution in a resorcinol/formaldehyde monomer aqueous system via an in situ emulsion polymerization procedure.
29851350	5	75	theme	superior	1302:1309	arg1	g-1					1336:1338	400 mg g-1	1329:1338	400 mg g-1	1329:1338	By taking advantage of the interaction between hydrophilic groups on the MMP and glycan components on the glycopeptides, the acquired MMP was utilized to the selective capture of N-glycopeptides (human IgG or HRP tryptic digests/BSA proteins = 1:50), good recovery yield (70.18-97.23%), superior binding capacity (400 mg g-1), and excellent reproducibility.
29851350	5	75	theme	superior	1302:1309	arg1	capacity					1319:1326	superior binding capacity	1302:1326	superior binding capacity (400 mg g-1)	1302:1339	By taking advantage of the interaction between hydrophilic groups on the MMP and glycan components on the glycopeptides, the acquired MMP was utilized to the selective capture of N-glycopeptides (human IgG or HRP tryptic digests/BSA proteins = 1:50), good recovery yield (70.18-97.23%), superior binding capacity (400 mg g-1), and excellent reproducibility.
29851350	2	76	theme	low	260:262	arg1	contents					264:271	the low contents	256:271	the low contents of glycopeptides compared with nonglycopeptides and the microheterogeneity of glycosylation sites	256:369	Due to the low contents of glycopeptides compared with nonglycopeptides and the microheterogeneity of glycosylation sites, highly selective enrichment methods for the purification of glycopeptides are required for the comprehensive characterization of glycoproteomics.
29851350	7	77	theme	sample	1619:1624	arg1	μL					1640:1641	0.025 μL	1634:1641	0.025 μL	1634:1641	A total of 15 unique N-glycopeptides were identified from an ultralow sample volume (0.025 μL) of human serum.
29851350	7	77	theme	sample	1619:1624	arg1	volume					1626:1631	an ultralow sample volume	1607:1631	an ultralow sample volume (0.025 μL) of human serum	1607:1657	A total of 15 unique N-glycopeptides were identified from an ultralow sample volume (0.025 μL) of human serum.
29851350	4	78	theme	solvent	952:958	arg1	resistance					960:969	solvent resistance	952:969	solvent resistance	952:969	The results showed that MMP exhibited good biocompatibility, a mesoporous structure, nitrogen-containing functionality, excellent hydrophilicity, and solvent resistance by using multiple characterization methods.
29851350	5	79	dep	=	1257:1257	arg1	1:50					1259:1262	1:50	1259:1262	1:50	1259:1262	By taking advantage of the interaction between hydrophilic groups on the MMP and glycan components on the glycopeptides, the acquired MMP was utilized to the selective capture of N-glycopeptides (human IgG or HRP tryptic digests/BSA proteins = 1:50), good recovery yield (70.18-97.23%), superior binding capacity (400 mg g-1), and excellent reproducibility.
29851350	5	80	theme	capacity	1319:1326	arg1	capture					1183:1189	the selective capture	1169:1189	the selective capture of N-glycopeptides (human IgG or HRP tryptic digests/BSA proteins = 1:50), good recovery yield (70.18-97.23%), superior binding capacity (400 mg g-1), and excellent reproducibility	1169:1370	By taking advantage of the interaction between hydrophilic groups on the MMP and glycan components on the glycopeptides, the acquired MMP was utilized to the selective capture of N-glycopeptides (human IgG or HRP tryptic digests/BSA proteins = 1:50), good recovery yield (70.18-97.23%), superior binding capacity (400 mg g-1), and excellent reproducibility.
29851350	6	81	theme	standard	1413:1420	arg1	digests					1444:1450	standard glycoproteins tryptic digests enrichment	1413:1461	standard glycoproteins tryptic digests enrichment	1413:1461	Based on the outstanding performance in standard glycoproteins tryptic digests enrichment, MMP was further used to capture N-glycopeptides from tryptic digests of human serum.
29851350	1	82	theme	protein	208:214	arg1	modification					235:246	a pivotal protein post-translational modification	198:246	a pivotal protein post-translational modification	198:246	Protein glycosylation is a significant participant in a mass of biological processes, which is a pivotal protein post-translational modification.
29851350	1	82	theme	protein	208:214	arg1	glycosylation					111:123	Protein glycosylation	103:123	Protein glycosylation	103:123	Protein glycosylation is a significant participant in a mass of biological processes, which is a pivotal protein post-translational modification.
29851350	1	82	theme	protein	208:214	arg1	participant					142:152	a significant participant	128:152	a significant participant	128:152	Protein glycosylation is a significant participant in a mass of biological processes, which is a pivotal protein post-translational modification.
29851350	5	83	from	components	1103:1112	arg1	glycopeptides					1121:1133	the glycopeptides	1117:1133	the glycopeptides	1117:1133	By taking advantage of the interaction between hydrophilic groups on the MMP and glycan components on the glycopeptides, the acquired MMP was utilized to the selective capture of N-glycopeptides (human IgG or HRP tryptic digests/BSA proteins = 1:50), good recovery yield (70.18-97.23%), superior binding capacity (400 mg g-1), and excellent reproducibility.
29851350	6	84	gly	N-glycopeptides	1496:1510	arg2	N-glycopeptides					1496:1510	N-glycopeptides	1496:1510	N-glycopeptides	1496:1510	Based on the outstanding performance in standard glycoproteins tryptic digests enrichment, MMP was further used to capture N-glycopeptides from tryptic digests of human serum.
29851350	6	85	from	performance	1398:1408	arg1	digests					1444:1450	standard glycoproteins tryptic digests enrichment	1413:1461	standard glycoproteins tryptic digests enrichment	1413:1461	Based on the outstanding performance in standard glycoproteins tryptic digests enrichment, MMP was further used to capture N-glycopeptides from tryptic digests of human serum.
29851350	0	86	theme	Glycopeptides	88:100	arg1	Enrichment					74:83	the Selective Enrichment	60:83	the Selective Enrichment of Glycopeptides	60:100	In Situ Synthesis of Magnetic Mesoporous Phenolic Resin for the Selective Enrichment of Glycopeptides.
29851350	5	87	theme	N-glycopeptides	1194:1208	arg1	capture					1183:1189	the selective capture	1169:1189	the selective capture of N-glycopeptides (human IgG or HRP tryptic digests/BSA proteins = 1:50), good recovery yield (70.18-97.23%), superior binding capacity (400 mg g-1), and excellent reproducibility	1169:1370	By taking advantage of the interaction between hydrophilic groups on the MMP and glycan components on the glycopeptides, the acquired MMP was utilized to the selective capture of N-glycopeptides (human IgG or HRP tryptic digests/BSA proteins = 1:50), good recovery yield (70.18-97.23%), superior binding capacity (400 mg g-1), and excellent reproducibility.
29851350	7	88	theme	N-glycopeptides	1570:1584	arg1	total					1551:1555	A total	1549:1555	A total of 15 unique N-glycopeptides	1549:1584	A total of 15 unique N-glycopeptides were identified from an ultralow sample volume (0.025 μL) of human serum.
29851350	5	89	dep	IgG	1217:1219	arg1	proteins					1248:1255	tryptic digests/BSA proteins	1228:1255	tryptic digests/BSA proteins	1228:1255	By taking advantage of the interaction between hydrophilic groups on the MMP and glycan components on the glycopeptides, the acquired MMP was utilized to the selective capture of N-glycopeptides (human IgG or HRP tryptic digests/BSA proteins = 1:50), good recovery yield (70.18-97.23%), superior binding capacity (400 mg g-1), and excellent reproducibility.
29851350	2	90	gly	glycopeptides	432:444	arg2	glycopeptides					432:444	glycopeptides	432:444	glycopeptides	432:444	Due to the low contents of glycopeptides compared with nonglycopeptides and the microheterogeneity of glycosylation sites, highly selective enrichment methods for the purification of glycopeptides are required for the comprehensive characterization of glycoproteomics.
29851350	6	91	gly	glycoproteins	1422:1434	arg1	glycoproteins					1422:1434	standard glycoproteins tryptic digests enrichment	1413:1461	standard glycoproteins tryptic digests enrichment	1413:1461	Based on the outstanding performance in standard glycoproteins tryptic digests enrichment, MMP was further used to capture N-glycopeptides from tryptic digests of human serum.
29851350	1	92	theme	post-translational	216:233	arg1	modification					235:246	a pivotal protein post-translational modification	198:246	a pivotal protein post-translational modification	198:246	Protein glycosylation is a significant participant in a mass of biological processes, which is a pivotal protein post-translational modification.
29851350	1	92	theme	post-translational	216:233	arg1	glycosylation					111:123	Protein glycosylation	103:123	Protein glycosylation	103:123	Protein glycosylation is a significant participant in a mass of biological processes, which is a pivotal protein post-translational modification.
29851350	1	92	theme	post-translational	216:233	arg1	participant					142:152	a significant participant	128:152	a significant participant	128:152	Protein glycosylation is a significant participant in a mass of biological processes, which is a pivotal protein post-translational modification.
29851350	5	93	theme	excellent	1346:1354	arg1	reproducibility					1356:1370	excellent reproducibility	1346:1370	excellent reproducibility	1346:1370	By taking advantage of the interaction between hydrophilic groups on the MMP and glycan components on the glycopeptides, the acquired MMP was utilized to the selective capture of N-glycopeptides (human IgG or HRP tryptic digests/BSA proteins = 1:50), good recovery yield (70.18-97.23%), superior binding capacity (400 mg g-1), and excellent reproducibility.
29851350	2	94	theme	comprehensive	467:479	arg1	characterization					481:496	the comprehensive characterization	463:496	the comprehensive characterization of glycoproteomics	463:515	Due to the low contents of glycopeptides compared with nonglycopeptides and the microheterogeneity of glycosylation sites, highly selective enrichment methods for the purification of glycopeptides are required for the comprehensive characterization of glycoproteomics.
29851350	5	95	theme	400	1329:1331	arg1	mg					1333:1334	mg	1333:1334	mg	1333:1334	By taking advantage of the interaction between hydrophilic groups on the MMP and glycan components on the glycopeptides, the acquired MMP was utilized to the selective capture of N-glycopeptides (human IgG or HRP tryptic digests/BSA proteins = 1:50), good recovery yield (70.18-97.23%), superior binding capacity (400 mg g-1), and excellent reproducibility.
29851350	5	96	theme	tryptic	1228:1234	arg1	proteins					1248:1255	tryptic digests/BSA proteins	1228:1255	tryptic digests/BSA proteins	1228:1255	By taking advantage of the interaction between hydrophilic groups on the MMP and glycan components on the glycopeptides, the acquired MMP was utilized to the selective capture of N-glycopeptides (human IgG or HRP tryptic digests/BSA proteins = 1:50), good recovery yield (70.18-97.23%), superior binding capacity (400 mg g-1), and excellent reproducibility.
29851350	3	97	theme	homogeneous	659:669	arg1	solution					691:698	a homogeneous magnetic Fe3O4@SiO2 solution	657:698	a homogeneous magnetic Fe3O4@SiO2 solution in a resorcinol/formaldehyde monomer aqueous system via an in situ emulsion polymerization procedure	657:799	In this work, a type of magnetic mesoporous phenolic resin (MMP) was prepared using branched polyethylenimine (PEI) as a cross-linker from a homogeneous magnetic Fe3O4@SiO2 solution in a resorcinol/formaldehyde monomer aqueous system via an in situ emulsion polymerization procedure.
29851350	6	98	theme	human	1536:1540	arg1	serum					1542:1546	human serum	1536:1546	human serum	1536:1546	Based on the outstanding performance in standard glycoproteins tryptic digests enrichment, MMP was further used to capture N-glycopeptides from tryptic digests of human serum.
29851350	2	99	theme	enrichment	389:398	arg1	methods					400:406	highly selective enrichment methods	372:406	highly selective enrichment methods for the purification of glycopeptides	372:444	Due to the low contents of glycopeptides compared with nonglycopeptides and the microheterogeneity of glycosylation sites, highly selective enrichment methods for the purification of glycopeptides are required for the comprehensive characterization of glycoproteomics.
29851350	0	100	theme	Mesoporous	30:39	arg1	Resin					50:54	Magnetic Mesoporous Phenolic Resin	21:54	Magnetic Mesoporous Phenolic Resin	21:54	In Situ Synthesis of Magnetic Mesoporous Phenolic Resin for the Selective Enrichment of Glycopeptides.
29851350	7	101	theme	unique	1563:1568	arg1	N-glycopeptides					1570:1584	15 unique N-glycopeptides	1560:1584	15 unique N-glycopeptides	1560:1584	A total of 15 unique N-glycopeptides were identified from an ultralow sample volume (0.025 μL) of human serum.
29851350	9	102	contain	has	1859:1861	arg1	MMP					1855:1857	MMP	1855:1857	MMP	1855:1857	All the results have demonstrated that MMP has great potential in large-scale N-glycoproteomics research.
29851350	9	102	contain	has	1859:1861	arg2	potential					1869:1877	great potential	1863:1877	great potential	1863:1877	All the results have demonstrated that MMP has great potential in large-scale N-glycoproteomics research.
29851350	4	103	theme	good	840:843	arg1	biocompatibility					845:860	good biocompatibility	840:860	good biocompatibility	840:860	The results showed that MMP exhibited good biocompatibility, a mesoporous structure, nitrogen-containing functionality, excellent hydrophilicity, and solvent resistance by using multiple characterization methods.
29851350	0	104	theme	Resin	50:54	arg1	Synthesis					8:16	In Situ Synthesis	0:16	In Situ Synthesis of Magnetic Mesoporous Phenolic Resin for the Selective Enrichment of Glycopeptides.	0:101	In Situ Synthesis of Magnetic Mesoporous Phenolic Resin for the Selective Enrichment of Glycopeptides.
29851350	5	105	theme	human	1211:1215	arg1	IgG					1217:1219	human IgG	1211:1219	human IgG	1211:1219	By taking advantage of the interaction between hydrophilic groups on the MMP and glycan components on the glycopeptides, the acquired MMP was utilized to the selective capture of N-glycopeptides (human IgG or HRP tryptic digests/BSA proteins = 1:50), good recovery yield (70.18-97.23%), superior binding capacity (400 mg g-1), and excellent reproducibility.
29851350	3	106	theme	SiO2	686:689	arg1	solution					691:698	a homogeneous magnetic Fe3O4@SiO2 solution	657:698	a homogeneous magnetic Fe3O4@SiO2 solution in a resorcinol/formaldehyde monomer aqueous system via an in situ emulsion polymerization procedure	657:799	In this work, a type of magnetic mesoporous phenolic resin (MMP) was prepared using branched polyethylenimine (PEI) as a cross-linker from a homogeneous magnetic Fe3O4@SiO2 solution in a resorcinol/formaldehyde monomer aqueous system via an in situ emulsion polymerization procedure.
29851350	2	107	theme	glycosylation	351:363	arg1	sites					365:369	glycosylation sites	351:369	glycosylation sites	351:369	Due to the low contents of glycopeptides compared with nonglycopeptides and the microheterogeneity of glycosylation sites, highly selective enrichment methods for the purification of glycopeptides are required for the comprehensive characterization of glycoproteomics.
29851350	3	108	theme	Fe3O4	680:684	arg1	solution					691:698	a homogeneous magnetic Fe3O4@SiO2 solution	657:698	a homogeneous magnetic Fe3O4@SiO2 solution in a resorcinol/formaldehyde monomer aqueous system via an in situ emulsion polymerization procedure	657:799	In this work, a type of magnetic mesoporous phenolic resin (MMP) was prepared using branched polyethylenimine (PEI) as a cross-linker from a homogeneous magnetic Fe3O4@SiO2 solution in a resorcinol/formaldehyde monomer aqueous system via an in situ emulsion polymerization procedure.
29851350	2	109	theme	selective	379:387	arg1	methods					400:406	highly selective enrichment methods	372:406	highly selective enrichment methods for the purification of glycopeptides	372:444	Due to the low contents of glycopeptides compared with nonglycopeptides and the microheterogeneity of glycosylation sites, highly selective enrichment methods for the purification of glycopeptides are required for the comprehensive characterization of glycoproteomics.
29851350	6	110	theme	tryptic	1517:1523	arg1	digests					1525:1531	tryptic digests	1517:1531	tryptic digests of human serum	1517:1546	Based on the outstanding performance in standard glycoproteins tryptic digests enrichment, MMP was further used to capture N-glycopeptides from tryptic digests of human serum.
29851350	5	111	theme	recovery	1271:1278	arg1	yield					1280:1284	good recovery yield	1266:1284	good recovery yield (70.18-97.23%)	1266:1299	By taking advantage of the interaction between hydrophilic groups on the MMP and glycan components on the glycopeptides, the acquired MMP was utilized to the selective capture of N-glycopeptides (human IgG or HRP tryptic digests/BSA proteins = 1:50), good recovery yield (70.18-97.23%), superior binding capacity (400 mg g-1), and excellent reproducibility.
29851350	5	111	theme	recovery	1271:1278	arg1	%					1298:1298	70.18-97.23%	1287:1298	70.18-97.23%	1287:1298	By taking advantage of the interaction between hydrophilic groups on the MMP and glycan components on the glycopeptides, the acquired MMP was utilized to the selective capture of N-glycopeptides (human IgG or HRP tryptic digests/BSA proteins = 1:50), good recovery yield (70.18-97.23%), superior binding capacity (400 mg g-1), and excellent reproducibility.
29851350	3	112	theme	resorcinol/formaldehyde	705:727	arg1	system					745:750	a resorcinol/formaldehyde monomer aqueous system	703:750	a resorcinol/formaldehyde monomer aqueous system	703:750	In this work, a type of magnetic mesoporous phenolic resin (MMP) was prepared using branched polyethylenimine (PEI) as a cross-linker from a homogeneous magnetic Fe3O4@SiO2 solution in a resorcinol/formaldehyde monomer aqueous system via an in situ emulsion polymerization procedure.
29851350	3	113	theme	mesoporous	551:560	arg1	MMP					578:580	MMP	578:580	MMP	578:580	In this work, a type of magnetic mesoporous phenolic resin (MMP) was prepared using branched polyethylenimine (PEI) as a cross-linker from a homogeneous magnetic Fe3O4@SiO2 solution in a resorcinol/formaldehyde monomer aqueous system via an in situ emulsion polymerization procedure.
29851350	3	113	theme	mesoporous	551:560	arg1	resin					571:575	magnetic mesoporous phenolic resin	542:575	magnetic mesoporous phenolic resin (MMP)	542:581	In this work, a type of magnetic mesoporous phenolic resin (MMP) was prepared using branched polyethylenimine (PEI) as a cross-linker from a homogeneous magnetic Fe3O4@SiO2 solution in a resorcinol/formaldehyde monomer aqueous system via an in situ emulsion polymerization procedure.
29851350	8	114	theme	unique	1687:1692	arg1	N-glycopeptides					1694:1708	356 unique N-glycopeptides	1683:1708	356 unique N-glycopeptides corresponding to 119 glycoproteins from human serum (0.35 μL) in the overlap of three replicate analyses	1683:1813	Overall, we identified 356 unique N-glycopeptides corresponding to 119 glycoproteins from human serum (0.35 μL) in the overlap of three replicate analyses.
29706962	5	0	theme	stable	885:890	arg1	antibodies					906:915	stable, glycosylated antibodies	885:915	stable, glycosylated antibodies	885:915	Strikingly, removal of Fab glycans naturally acquired during antigen-specific immune responses can deteriorate antibody stability, suggesting in vivo selection of stable, glycosylated antibodies.
29706962	5	1	theme	antigen-specific	783:798	arg1	responses					807:815	antigen-specific immune responses	783:815	antigen-specific immune responses	783:815	Strikingly, removal of Fab glycans naturally acquired during antigen-specific immune responses can deteriorate antibody stability, suggesting in vivo selection of stable, glycosylated antibodies.
29706962	7	2	theme	therapeutic/diagnostic	1154:1175	arg1	stability					1186:1194	therapeutic/diagnostic antibody stability	1154:1194	therapeutic/diagnostic antibody stability	1154:1194	These findings indicate that introducing Fab glycans may represent a mechanism to improve therapeutic/diagnostic antibody stability.
29706962	6	3	theme	N-linked	967:974	arg1	glycans					976:982	variable domain N-linked glycans	951:982	variable domain N-linked glycans acquired during somatic hypermutation	951:1020	Collectively, our data show that variable domain N-linked glycans acquired during somatic hypermutation can contribute to IgG antibody stability.
29706962	7	4	theme	antibody	1177:1184	arg1	stability					1186:1194	therapeutic/diagnostic antibody stability	1154:1194	therapeutic/diagnostic antibody stability	1154:1194	These findings indicate that introducing Fab glycans may represent a mechanism to improve therapeutic/diagnostic antibody stability.
29706962	0	5	theme	G	116:116	arg1	Stability					127:135	Immunoglobulin G Antibody Stability	101:135	Immunoglobulin G Antibody Stability	101:135	Variable Domain N-Linked Glycans Acquired During Antigen-Specific Immune Responses Can Contribute to Immunoglobulin G Antibody Stability.
29706962	6	6	theme	domain	960:965	arg1	glycans					976:982	variable domain N-linked glycans	951:982	variable domain N-linked glycans acquired during somatic hypermutation	951:1020	Collectively, our data show that variable domain N-linked glycans acquired during somatic hypermutation can contribute to IgG antibody stability.
29706962	5	7	dep	in	864:865	arg1	vivo					867:870	vivo	867:870	vivo	867:870	Strikingly, removal of Fab glycans naturally acquired during antigen-specific immune responses can deteriorate antibody stability, suggesting in vivo selection of stable, glycosylated antibodies.
29706962	6	8	theme	variable	951:958	arg1	glycans					976:982	variable domain N-linked glycans	951:982	variable domain N-linked glycans acquired during somatic hypermutation	951:1020	Collectively, our data show that variable domain N-linked glycans acquired during somatic hypermutation can contribute to IgG antibody stability.
29706962	4	9	theme	Fab	629:631	arg1	glycans					633:639	Fab glycans	629:639	Fab glycans	629:639	By analyzing thermal unfolding profiles of antibodies with or without Fab glycans, we demonstrate that introduction of Fab glycans can improve antibody stability.
29706962	0	10	theme	Immunoglobulin	101:114	arg1	Stability					127:135	Immunoglobulin G Antibody Stability	101:135	Immunoglobulin G Antibody Stability	101:135	Variable Domain N-Linked Glycans Acquired During Antigen-Specific Immune Responses Can Contribute to Immunoglobulin G Antibody Stability.
29706962	1	11	contain	contain	165:171	arg1	IgG					156:158	IgG	156:158	IgG	156:158	Immunoglobulin G (IgG) can contain N-linked glycans in the variable domains, the so-called Fab glycans, in addition to the Fc glycans in the CH2 domains.
29706962	1	11	contain	contain	165:171	arg1	glycans					233:239	the so-called Fab glycans	215:239	the so-called Fab glycans	215:239	Immunoglobulin G (IgG) can contain N-linked glycans in the variable domains, the so-called Fab glycans, in addition to the Fc glycans in the CH2 domains.
29706962	1	11	contain	contain	165:171	arg2	glycans					182:188	N-linked glycans	173:188	N-linked glycans	173:188	Immunoglobulin G (IgG) can contain N-linked glycans in the variable domains, the so-called Fab glycans, in addition to the Fc glycans in the CH2 domains.
29706962	1	11	contain	contain	165:171	arg2	G					153:153	Immunoglobulin G	138:153	Immunoglobulin G (IgG)	138:159	Immunoglobulin G (IgG) can contain N-linked glycans in the variable domains, the so-called Fab glycans, in addition to the Fc glycans in the CH2 domains.
29706962	1	11	contain	contain	165:171	arg1	domains					206:212	the variable domains	193:212	the variable domains	193:212	Immunoglobulin G (IgG) can contain N-linked glycans in the variable domains, the so-called Fab glycans, in addition to the Fc glycans in the CH2 domains.
29706962	1	11	contain	contain	165:171	arg2	IgG					156:158	IgG	156:158	IgG	156:158	Immunoglobulin G (IgG) can contain N-linked glycans in the variable domains, the so-called Fab glycans, in addition to the Fc glycans in the CH2 domains.
29706962	1	11	contain	contain	165:171	arg1	G					153:153	Immunoglobulin G	138:153	Immunoglobulin G (IgG)	138:159	Immunoglobulin G (IgG) can contain N-linked glycans in the variable domains, the so-called Fab glycans, in addition to the Fc glycans in the CH2 domains.
29706962	6	12	theme	somatic	1000:1006	arg1	hypermutation					1008:1020	somatic hypermutation	1000:1020	somatic hypermutation	1000:1020	Collectively, our data show that variable domain N-linked glycans acquired during somatic hypermutation can contribute to IgG antibody stability.
29706962	1	13	theme	N-linked	173:180	arg1	glycans					182:188	N-linked glycans	173:188	N-linked glycans	173:188	Immunoglobulin G (IgG) can contain N-linked glycans in the variable domains, the so-called Fab glycans, in addition to the Fc glycans in the CH2 domains.
29706962	0	14	theme	Antibody	118:125	arg1	Stability					127:135	Immunoglobulin G Antibody Stability	101:135	Immunoglobulin G Antibody Stability	101:135	Variable Domain N-Linked Glycans Acquired During Antigen-Specific Immune Responses Can Contribute to Immunoglobulin G Antibody Stability.
29706962	2	15	theme	somatic	378:384	arg1	hypermutation					386:398	somatic hypermutation	378:398	somatic hypermutation	378:398	These Fab glycans are acquired following introduction of N-glycosylation sites during somatic hypermutation and contribute to antibody diversification.
29706962	0	16	theme	Domain	9:14	arg1	Glycans					25:31	Variable Domain N-Linked Glycans	0:31	Variable Domain N-Linked Glycans Acquired During Antigen-Specific Immune Responses	0:81	Variable Domain N-Linked Glycans Acquired During Antigen-Specific Immune Responses Can Contribute to Immunoglobulin G Antibody Stability.
29706962	1	17	link	N-linked	173:180	arg1	glycans					182:188	N-linked glycans	173:188	N-linked glycans	173:188	Immunoglobulin G (IgG) can contain N-linked glycans in the variable domains, the so-called Fab glycans, in addition to the Fc glycans in the CH2 domains.
29706962	6	18	theme	antibody	1044:1051	arg1	stability					1053:1061	IgG antibody stability	1040:1061	IgG antibody stability	1040:1061	Collectively, our data show that variable domain N-linked glycans acquired during somatic hypermutation can contribute to IgG antibody stability.
29706962	4	19	theme	antibody	702:709	arg1	stability					711:719	antibody stability	702:719	antibody stability	702:719	By analyzing thermal unfolding profiles of antibodies with or without Fab glycans, we demonstrate that introduction of Fab glycans can improve antibody stability.
29706962	0	20	theme	Variable	0:7	arg1	Glycans					25:31	Variable Domain N-Linked Glycans	0:31	Variable Domain N-Linked Glycans Acquired During Antigen-Specific Immune Responses	0:81	Variable Domain N-Linked Glycans Acquired During Antigen-Specific Immune Responses Can Contribute to Immunoglobulin G Antibody Stability.
29706962	1	21	theme	Fc	261:262	arg1	glycans					264:270	the Fc glycans	257:270	the Fc glycans in the CH2 domains	257:289	Immunoglobulin G (IgG) can contain N-linked glycans in the variable domains, the so-called Fab glycans, in addition to the Fc glycans in the CH2 domains.
29706962	1	22	from	glycans	264:270	arg1	domains					283:289	the CH2 domains	275:289	the CH2 domains	275:289	Immunoglobulin G (IgG) can contain N-linked glycans in the variable domains, the so-called Fab glycans, in addition to the Fc glycans in the CH2 domains.
29706962	2	23	theme	sites	365:369	arg1	introduction					333:344	introduction	333:344	introduction of N-glycosylation sites	333:369	These Fab glycans are acquired following introduction of N-glycosylation sites during somatic hypermutation and contribute to antibody diversification.
29706962	4	24	theme	unfolding	580:588	arg1	profiles					590:597	thermal unfolding profiles	572:597	thermal unfolding profiles of antibodies with or without Fab glycans	572:639	By analyzing thermal unfolding profiles of antibodies with or without Fab glycans, we demonstrate that introduction of Fab glycans can improve antibody stability.
29706962	2	25	theme	antibody	418:425	arg1	diversification					427:441	antibody diversification	418:441	antibody diversification	418:441	These Fab glycans are acquired following introduction of N-glycosylation sites during somatic hypermutation and contribute to antibody diversification.
29706962	0	26	theme	N-Linked	16:23	arg1	Glycans					25:31	Variable Domain N-Linked Glycans	0:31	Variable Domain N-Linked Glycans Acquired During Antigen-Specific Immune Responses	0:81	Variable Domain N-Linked Glycans Acquired During Antigen-Specific Immune Responses Can Contribute to Immunoglobulin G Antibody Stability.
29706962	4	27	theme	antibodies	602:611	arg1	profiles					590:597	thermal unfolding profiles	572:597	thermal unfolding profiles of antibodies with or without Fab glycans	572:639	By analyzing thermal unfolding profiles of antibodies with or without Fab glycans, we demonstrate that introduction of Fab glycans can improve antibody stability.
29706962	2	28	gly	N-glycosylation	349:363	arg2	sites					365:369	N-glycosylation sites	349:369	N-glycosylation sites	349:369	These Fab glycans are acquired following introduction of N-glycosylation sites during somatic hypermutation and contribute to antibody diversification.
29706962	3	29	theme	antibody	539:546	arg1	stability					548:556	antibody stability	539:556	antibody stability	539:556	We investigated whether Fab glycans may-in addition to affecting antigen binding-contribute to antibody stability.
29706962	5	30	theme	antibody	833:840	arg1	stability					842:850	antibody stability	833:850	antibody stability	833:850	Strikingly, removal of Fab glycans naturally acquired during antigen-specific immune responses can deteriorate antibody stability, suggesting in vivo selection of stable, glycosylated antibodies.
29706962	3	31	theme	antigen	509:515	arg1	binding-contribute					517:534	antigen binding-contribute	509:534	antigen binding-contribute to antibody stability	509:556	We investigated whether Fab glycans may-in addition to affecting antigen binding-contribute to antibody stability.
29706962	5	32	theme	Fab	745:747	arg1	glycans					749:755	Fab glycans	745:755	Fab glycans naturally acquired during antigen-specific immune responses	745:815	Strikingly, removal of Fab glycans naturally acquired during antigen-specific immune responses can deteriorate antibody stability, suggesting in vivo selection of stable, glycosylated antibodies.
29706962	4	33	theme	Fab	678:680	arg1	glycans					682:688	Fab glycans	678:688	Fab glycans	678:688	By analyzing thermal unfolding profiles of antibodies with or without Fab glycans, we demonstrate that introduction of Fab glycans can improve antibody stability.
29706962	6	34	link	N-linked	967:974	arg1	glycans					976:982	variable domain N-linked glycans	951:982	variable domain N-linked glycans acquired during somatic hypermutation	951:1020	Collectively, our data show that variable domain N-linked glycans acquired during somatic hypermutation can contribute to IgG antibody stability.
29706962	1	35	theme	CH2	279:281	arg1	domains					283:289	the CH2 domains	275:289	the CH2 domains	275:289	Immunoglobulin G (IgG) can contain N-linked glycans in the variable domains, the so-called Fab glycans, in addition to the Fc glycans in the CH2 domains.
29706962	5	36	theme	glycans	749:755	arg1	removal					734:740	removal	734:740	removal of Fab glycans naturally acquired during antigen-specific immune responses	734:815	Strikingly, removal of Fab glycans naturally acquired during antigen-specific immune responses can deteriorate antibody stability, suggesting in vivo selection of stable, glycosylated antibodies.
29706962	3	37	theme	Fab	468:470	arg1	glycans					472:478	Fab glycans	468:478	Fab glycans	468:478	We investigated whether Fab glycans may-in addition to affecting antigen binding-contribute to antibody stability.
29706962	0	38	theme	Immune	66:71	arg1	Responses					73:81	Antigen-Specific Immune Responses	49:81	Antigen-Specific Immune Responses	49:81	Variable Domain N-Linked Glycans Acquired During Antigen-Specific Immune Responses Can Contribute to Immunoglobulin G Antibody Stability.
29706962	6	39	theme	IgG	1040:1042	arg1	stability					1053:1061	IgG antibody stability	1040:1061	IgG antibody stability	1040:1061	Collectively, our data show that variable domain N-linked glycans acquired during somatic hypermutation can contribute to IgG antibody stability.
29706962	7	40	theme	Fab	1105:1107	arg1	glycans					1109:1115	Fab glycans	1105:1115	Fab glycans	1105:1115	These findings indicate that introducing Fab glycans may represent a mechanism to improve therapeutic/diagnostic antibody stability.
29706962	0	41	theme	Antigen-Specific	49:64	arg1	Responses					73:81	Antigen-Specific Immune Responses	49:81	Antigen-Specific Immune Responses	49:81	Variable Domain N-Linked Glycans Acquired During Antigen-Specific Immune Responses Can Contribute to Immunoglobulin G Antibody Stability.
29706962	4	42	theme	thermal	572:578	arg1	profiles					590:597	thermal unfolding profiles	572:597	thermal unfolding profiles of antibodies with or without Fab glycans	572:639	By analyzing thermal unfolding profiles of antibodies with or without Fab glycans, we demonstrate that introduction of Fab glycans can improve antibody stability.
29706962	1	43	theme	Immunoglobulin	138:151	arg1	G					153:153	Immunoglobulin G	138:153	Immunoglobulin G (IgG)	138:159	Immunoglobulin G (IgG) can contain N-linked glycans in the variable domains, the so-called Fab glycans, in addition to the Fc glycans in the CH2 domains.
29706962	1	43	theme	Immunoglobulin	138:151	arg1	IgG					156:158	IgG	156:158	IgG	156:158	Immunoglobulin G (IgG) can contain N-linked glycans in the variable domains, the so-called Fab glycans, in addition to the Fc glycans in the CH2 domains.
29706962	1	44	theme	so-called	219:227	arg1	glycans					233:239	the so-called Fab glycans	215:239	the so-called Fab glycans	215:239	Immunoglobulin G (IgG) can contain N-linked glycans in the variable domains, the so-called Fab glycans, in addition to the Fc glycans in the CH2 domains.
29706962	1	44	theme	so-called	219:227	arg1	domains					206:212	the variable domains	193:212	the variable domains	193:212	Immunoglobulin G (IgG) can contain N-linked glycans in the variable domains, the so-called Fab glycans, in addition to the Fc glycans in the CH2 domains.
29706962	2	45	theme	Fab	298:300	arg1	glycans					302:308	These Fab glycans	292:308	These Fab glycans	292:308	These Fab glycans are acquired following introduction of N-glycosylation sites during somatic hypermutation and contribute to antibody diversification.
29706962	1	46	theme	Fab	229:231	arg1	glycans					233:239	the so-called Fab glycans	215:239	the so-called Fab glycans	215:239	Immunoglobulin G (IgG) can contain N-linked glycans in the variable domains, the so-called Fab glycans, in addition to the Fc glycans in the CH2 domains.
29706962	1	46	theme	Fab	229:231	arg1	domains					206:212	the variable domains	193:212	the variable domains	193:212	Immunoglobulin G (IgG) can contain N-linked glycans in the variable domains, the so-called Fab glycans, in addition to the Fc glycans in the CH2 domains.
29706962	5	47	theme	immune	800:805	arg1	responses					807:815	antigen-specific immune responses	783:815	antigen-specific immune responses	783:815	Strikingly, removal of Fab glycans naturally acquired during antigen-specific immune responses can deteriorate antibody stability, suggesting in vivo selection of stable, glycosylated antibodies.
29706962	5	48	theme	antibodies	906:915	arg1	selection					872:880	in vivo selection	864:880	in vivo selection of stable, glycosylated antibodies	864:915	Strikingly, removal of Fab glycans naturally acquired during antigen-specific immune responses can deteriorate antibody stability, suggesting in vivo selection of stable, glycosylated antibodies.
29706962	5	49	dep	stable	885:890	arg1	glycosylated					893:904	glycosylated	893:904	glycosylated	893:904	Strikingly, removal of Fab glycans naturally acquired during antigen-specific immune responses can deteriorate antibody stability, suggesting in vivo selection of stable, glycosylated antibodies.
29706962	1	50	theme	variable	197:204	arg1	glycans					233:239	the so-called Fab glycans	215:239	the so-called Fab glycans	215:239	Immunoglobulin G (IgG) can contain N-linked glycans in the variable domains, the so-called Fab glycans, in addition to the Fc glycans in the CH2 domains.
29706962	1	50	theme	variable	197:204	arg1	domains					206:212	the variable domains	193:212	the variable domains	193:212	Immunoglobulin G (IgG) can contain N-linked glycans in the variable domains, the so-called Fab glycans, in addition to the Fc glycans in the CH2 domains.
29706962	2	51	theme	N-glycosylation	349:363	arg1	sites					365:369	N-glycosylation sites	349:369	N-glycosylation sites	349:369	These Fab glycans are acquired following introduction of N-glycosylation sites during somatic hypermutation and contribute to antibody diversification.
29706962	4	52	theme	glycans	682:688	arg1	introduction					662:673	introduction	662:673	introduction of Fab glycans	662:688	By analyzing thermal unfolding profiles of antibodies with or without Fab glycans, we demonstrate that introduction of Fab glycans can improve antibody stability.
29706962	5	53	theme	in	864:865	arg1	selection					872:880	in vivo selection	864:880	in vivo selection of stable, glycosylated antibodies	864:915	Strikingly, removal of Fab glycans naturally acquired during antigen-specific immune responses can deteriorate antibody stability, suggesting in vivo selection of stable, glycosylated antibodies.
31475984	5	0	link	cross-linked	966:977	arg1	agarose					979:985	activated cross-linked agarose	956:985	activated cross-linked agarose	956:985	Furthermore, a method based on activated cross-linked agarose was optimized for high ligand density, efficient coupling and low costs.
31475984	3	1	theme	epitope	516:522	arg1	ligand					524:529	a DsRed-based epitope ligand	502:529	a DsRed-based epitope ligand	502:529	Here we present a method for the immobilization of a DsRed-based epitope ligand to a cross-linked agarose resin allowing the selective capture of the HIV-neutralizing antibody 2F5 from crude plant extracts without using Protein A.
31475984	7	2	theme	affinity	1449:1456	arg1	stability					1464:1472	affinity resin stability	1449:1472	affinity resin stability	1449:1472	The resulting affinity resin was tested for its ability to selectively bind the target mAb in a crude plant extract and the elution buffer was optimized for high mAb recovery, product activity and affinity resin stability.
31475984	4	3	theme	tobacco	820:826	arg1	plants					848:853	transgenic tobacco (Nicotiana tabacum) plants	809:853	transgenic tobacco (Nicotiana tabacum) plants	809:853	The linear epitope ELDKWA was first genetically fused to the fluorescent protein DsRed and the fusion protein was expressed in transgenic tobacco (Nicotiana tabacum) plants before purification by immobilized metal-ion affinity chromatography.
31475984	7	4	theme	high	1409:1412	arg1	recovery					1418:1425	high mAb recovery	1409:1425	high mAb recovery	1409:1425	The resulting affinity resin was tested for its ability to selectively bind the target mAb in a crude plant extract and the elution buffer was optimized for high mAb recovery, product activity and affinity resin stability.
31475984	4	5	theme	Nicotiana	829:837	arg1	plants					848:853	transgenic tobacco (Nicotiana tabacum) plants	809:853	transgenic tobacco (Nicotiana tabacum) plants	809:853	The linear epitope ELDKWA was first genetically fused to the fluorescent protein DsRed and the fusion protein was expressed in transgenic tobacco (Nicotiana tabacum) plants before purification by immobilized metal-ion affinity chromatography.
31475984	3	6	theme	cross-linked	536:547	arg1	resin					557:561	a cross-linked agarose resin	534:561	a cross-linked agarose resin allowing the selective capture of the HIV-neutralizing antibody 2F5 from crude plant extracts without using Protein A	534:679	Here we present a method for the immobilization of a DsRed-based epitope ligand to a cross-linked agarose resin allowing the selective capture of the HIV-neutralizing antibody 2F5 from crude plant extracts without using Protein A.
31475984	9	7	theme	step	1667:1670	arg1	costs					1639:1643	the costs	1635:1643	the costs of an initial capture step for mAb production	1635:1689	The new resins allow gentler elution conditions than Protein A and could also reduce the costs of an initial capture step for mAb production.
31475984	0	8	theme	Case	115:118	arg1	Study					120:124	a Case Study	113:124	a Case Study	113:124	Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study.
31475984	9	9	theme	mAb	1676:1678	arg1	production					1680:1689	mAb production	1676:1689	mAb production	1676:1689	The new resins allow gentler elution conditions than Protein A and could also reduce the costs of an initial capture step for mAb production.
31475984	1	10	theme	Protein	200:206	arg1	chromatography					219:232	Protein A affinity chromatography	200:232	Protein A affinity chromatography	200:232	The purification of monoclonal antibodies (mAbs) is commonly achieved by Protein A affinity chromatography, which can account for up to 25% of the overall process costs.
31475984	4	11	theme	linear	686:691	arg1	ELDKWA					701:706	The linear epitope ELDKWA	682:706	The linear epitope ELDKWA	682:706	The linear epitope ELDKWA was first genetically fused to the fluorescent protein DsRed and the fusion protein was expressed in transgenic tobacco (Nicotiana tabacum) plants before purification by immobilized metal-ion affinity chromatography.
31475984	4	12	theme	metal-ion	890:898	arg1	chromatography					909:922	immobilized metal-ion affinity chromatography	878:922	immobilized metal-ion affinity chromatography	878:922	The linear epitope ELDKWA was first genetically fused to the fluorescent protein DsRed and the fusion protein was expressed in transgenic tobacco (Nicotiana tabacum) plants before purification by immobilized metal-ion affinity chromatography.
31475984	7	13	theme	crude	1348:1352	arg1	extract					1360:1366	a crude plant extract	1346:1366	a crude plant extract	1346:1366	The resulting affinity resin was tested for its ability to selectively bind the target mAb in a crude plant extract and the elution buffer was optimized for high mAb recovery, product activity and affinity resin stability.
31475984	5	14	theme	low	1049:1051	arg1	costs					1053:1057	low costs	1049:1057	low costs	1049:1057	Furthermore, a method based on activated cross-linked agarose was optimized for high ligand density, efficient coupling and low costs.
31475984	8	15	with	antibodies	1517:1526	arg1	epitopes					1540:1547	linear epitopes	1533:1547	linear epitopes	1533:1547	The method can easily be adapted to other antibodies with linear epitopes.
31475984	0	16	theme	Affinity	60:67	arg1	Chromatography					69:82	Affinity Chromatography	60:82	Affinity Chromatography Resins	60:89	Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study.
31475984	1	17	theme	costs	290:294	arg1	costs					290:294	the overall process costs	270:294	the overall process costs	270:294	The purification of monoclonal antibodies (mAbs) is commonly achieved by Protein A affinity chromatography, which can account for up to 25% of the overall process costs.
31475984	1	17	theme	costs	290:294	arg1	%					265:265	up to 25%	257:265	up to 25% of the overall process costs	257:294	The purification of monoclonal antibodies (mAbs) is commonly achieved by Protein A affinity chromatography, which can account for up to 25% of the overall process costs.
31475984	0	18	theme	Resins	84:89	arg1	Development					45:55	the Rapid Development	35:55	the Rapid Development of Affinity Chromatography Resins	35:89	Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study.
31475984	1	19	theme	antibodies	158:167	arg1	purification					131:142	The purification	127:142	The purification of monoclonal antibodies (mAbs)	127:174	The purification of monoclonal antibodies (mAbs) is commonly achieved by Protein A affinity chromatography, which can account for up to 25% of the overall process costs.
31475984	0	20	theme	Antibody	93:100	arg1	Capture					102:108	Antibody Capture	93:108	Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study.	0:125	Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study.
31475984	5	21	theme	activated	956:964	arg1	agarose					979:985	activated cross-linked agarose	956:985	activated cross-linked agarose	956:985	Furthermore, a method based on activated cross-linked agarose was optimized for high ligand density, efficient coupling and low costs.
31475984	4	22	theme	protein	755:761	arg1	DsRed					763:767	the fluorescent protein DsRed	739:767	the fluorescent protein DsRed	739:767	The linear epitope ELDKWA was first genetically fused to the fluorescent protein DsRed and the fusion protein was expressed in transgenic tobacco (Nicotiana tabacum) plants before purification by immobilized metal-ion affinity chromatography.
31475984	2	23	theme	industrial-scale	366:381	arg1	manufacturing					383:395	industrial-scale manufacturing	366:395	industrial-scale manufacturing	366:395	Alternative, cost-effective capture steps are therefore valuable for industrial-scale manufacturing, where large quantities of a single mAb are produced.
31475984	2	24	theme	single	426:431	arg1	mAb					433:435	a single mAb	424:435	a single mAb	424:435	Alternative, cost-effective capture steps are therefore valuable for industrial-scale manufacturing, where large quantities of a single mAb are produced.
31475984	8	25	theme	other	1511:1515	arg1	antibodies					1517:1526	other antibodies	1511:1526	other antibodies with linear epitopes	1511:1547	The method can easily be adapted to other antibodies with linear epitopes.
31475984	9	26	theme	initial	1651:1657	arg1	step					1667:1670	an initial capture step	1648:1670	an initial capture step	1648:1670	The new resins allow gentler elution conditions than Protein A and could also reduce the costs of an initial capture step for mAb production.
31475984	0	27	theme	Activated	0:8	arg1	Agarose					23:29	Activated Cross-linked Agarose	0:29	Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study.	0:125	Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study.
31475984	9	28	theme	new	1554:1556	arg1	resins					1558:1563	The new resins	1550:1563	The new resins	1550:1563	The new resins allow gentler elution conditions than Protein A and could also reduce the costs of an initial capture step for mAb production.
31475984	5	29	theme	ligand	1010:1015	arg1	density					1017:1023	high ligand density	1005:1023	high ligand density	1005:1023	Furthermore, a method based on activated cross-linked agarose was optimized for high ligand density, efficient coupling and low costs.
31475984	2	30	theme	large	404:408	arg1	quantities					410:419	large quantities	404:419	large quantities of a single mAb	404:435	Alternative, cost-effective capture steps are therefore valuable for industrial-scale manufacturing, where large quantities of a single mAb are produced.
31475984	9	31	theme	elution	1579:1585	arg1	conditions					1587:1596	gentler elution conditions	1571:1596	gentler elution conditions	1571:1596	The new resins allow gentler elution conditions than Protein A and could also reduce the costs of an initial capture step for mAb production.
31475984	3	32	theme	antibody	618:625	arg1	2F5					627:629	the HIV-neutralizing antibody 2F5	597:629	the HIV-neutralizing antibody 2F5	597:629	Here we present a method for the immobilization of a DsRed-based epitope ligand to a cross-linked agarose resin allowing the selective capture of the HIV-neutralizing antibody 2F5 from crude plant extracts without using Protein A.
31475984	1	33	theme	overall	274:280	arg1	costs					290:294	the overall process costs	270:294	the overall process costs	270:294	The purification of monoclonal antibodies (mAbs) is commonly achieved by Protein A affinity chromatography, which can account for up to 25% of the overall process costs.
31475984	5	34	theme	efficient	1026:1034	arg1	coupling					1036:1043	efficient coupling	1026:1043	efficient coupling	1026:1043	Furthermore, a method based on activated cross-linked agarose was optimized for high ligand density, efficient coupling and low costs.
31475984	1	35	theme	A	208:208	arg1	chromatography					219:232	Protein A affinity chromatography	200:232	Protein A affinity chromatography	200:232	The purification of monoclonal antibodies (mAbs) is commonly achieved by Protein A affinity chromatography, which can account for up to 25% of the overall process costs.
31475984	0	36	dep	Agarose	23:29	arg1	Capture					102:108	Antibody Capture	93:108	Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study.	0:125	Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study.
31475984	6	37	theme	soluble	1098:1104	arg1	ligand					1106:1111	the soluble ligand	1094:1111	the soluble ligand concentration	1094:1125	The pH and buffer composition and the soluble ligand concentration were the most important parameters during the coupling procedure, which was improved using a design-of-experiments approach.
31475984	3	38	theme	plant	642:646	arg1	extracts					648:655	crude plant extracts	636:655	crude plant extracts	636:655	Here we present a method for the immobilization of a DsRed-based epitope ligand to a cross-linked agarose resin allowing the selective capture of the HIV-neutralizing antibody 2F5 from crude plant extracts without using Protein A.
31475984	4	39	theme	fusion	777:782	arg1	protein					784:790	the fusion protein	773:790	the fusion protein	773:790	The linear epitope ELDKWA was first genetically fused to the fluorescent protein DsRed and the fusion protein was expressed in transgenic tobacco (Nicotiana tabacum) plants before purification by immobilized metal-ion affinity chromatography.
31475984	5	40	theme	high	1005:1008	arg1	density					1017:1023	high ligand density	1005:1023	high ligand density	1005:1023	Furthermore, a method based on activated cross-linked agarose was optimized for high ligand density, efficient coupling and low costs.
31475984	7	41	theme	affinity	1266:1273	arg1	resin					1275:1279	The resulting affinity resin	1252:1279	The resulting affinity resin	1252:1279	The resulting affinity resin was tested for its ability to selectively bind the target mAb in a crude plant extract and the elution buffer was optimized for high mAb recovery, product activity and affinity resin stability.
31475984	3	42	from	extracts	648:655	arg1	capture					586:592	the selective capture	572:592	the selective capture of the HIV-neutralizing antibody 2F5 from crude plant extracts	572:655	Here we present a method for the immobilization of a DsRed-based epitope ligand to a cross-linked agarose resin allowing the selective capture of the HIV-neutralizing antibody 2F5 from crude plant extracts without using Protein A.
31475984	2	43	theme	capture	325:331	arg1	steps					333:337	Alternative, cost-effective capture steps	297:337	Alternative, cost-effective capture steps	297:337	Alternative, cost-effective capture steps are therefore valuable for industrial-scale manufacturing, where large quantities of a single mAb are produced.
31475984	7	44	theme	resin	1458:1462	arg1	stability					1464:1472	affinity resin stability	1449:1472	affinity resin stability	1449:1472	The resulting affinity resin was tested for its ability to selectively bind the target mAb in a crude plant extract and the elution buffer was optimized for high mAb recovery, product activity and affinity resin stability.
31475984	4	45	theme	tabacum	839:845	arg1	plants					848:853	transgenic tobacco (Nicotiana tabacum) plants	809:853	transgenic tobacco (Nicotiana tabacum) plants	809:853	The linear epitope ELDKWA was first genetically fused to the fluorescent protein DsRed and the fusion protein was expressed in transgenic tobacco (Nicotiana tabacum) plants before purification by immobilized metal-ion affinity chromatography.
31475984	6	46	theme	buffer	1071:1076	arg1	concentration					1113:1125	the soluble ligand concentration	1094:1125	the soluble ligand concentration	1094:1125	The pH and buffer composition and the soluble ligand concentration were the most important parameters during the coupling procedure, which was improved using a design-of-experiments approach.
31475984	6	46	theme	buffer	1071:1076	arg1	parameters					1151:1160	the most important parameters	1132:1160	the most important parameters	1132:1160	The pH and buffer composition and the soluble ligand concentration were the most important parameters during the coupling procedure, which was improved using a design-of-experiments approach.
31475984	6	46	theme	buffer	1071:1076	arg1	pH					1064:1065	The pH	1060:1065	The pH	1060:1065	The pH and buffer composition and the soluble ligand concentration were the most important parameters during the coupling procedure, which was improved using a design-of-experiments approach.
31475984	6	46	theme	buffer	1071:1076	arg1	composition					1078:1088	buffer composition	1071:1088	buffer composition	1071:1088	The pH and buffer composition and the soluble ligand concentration were the most important parameters during the coupling procedure, which was improved using a design-of-experiments approach.
31475984	3	47	theme	Protein	671:677	arg1	A					679:679	Protein A	671:679	Protein A	671:679	Here we present a method for the immobilization of a DsRed-based epitope ligand to a cross-linked agarose resin allowing the selective capture of the HIV-neutralizing antibody 2F5 from crude plant extracts without using Protein A.
31475984	3	48	theme	DsRed-based	504:514	arg1	ligand					524:529	a DsRed-based epitope ligand	502:529	a DsRed-based epitope ligand	502:529	Here we present a method for the immobilization of a DsRed-based epitope ligand to a cross-linked agarose resin allowing the selective capture of the HIV-neutralizing antibody 2F5 from crude plant extracts without using Protein A.
31475984	0	49	link	Cross-linked	10:21	arg1	Agarose					23:29	Activated Cross-linked Agarose	0:29	Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study.	0:125	Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study.
31475984	6	50	theme	coupling	1173:1180	arg1	procedure					1182:1190	the coupling procedure	1169:1190	the coupling procedure	1169:1190	The pH and buffer composition and the soluble ligand concentration were the most important parameters during the coupling procedure, which was improved using a design-of-experiments approach.
31475984	7	51	theme	product	1428:1434	arg1	activity					1436:1443	product activity	1428:1443	product activity	1428:1443	The resulting affinity resin was tested for its ability to selectively bind the target mAb in a crude plant extract and the elution buffer was optimized for high mAb recovery, product activity and affinity resin stability.
31475984	4	52	theme	transgenic	809:818	arg1	plants					848:853	transgenic tobacco (Nicotiana tabacum) plants	809:853	transgenic tobacco (Nicotiana tabacum) plants	809:853	The linear epitope ELDKWA was first genetically fused to the fluorescent protein DsRed and the fusion protein was expressed in transgenic tobacco (Nicotiana tabacum) plants before purification by immobilized metal-ion affinity chromatography.
31475984	7	53	theme	mAb	1414:1416	arg1	recovery					1418:1425	high mAb recovery	1409:1425	high mAb recovery	1409:1425	The resulting affinity resin was tested for its ability to selectively bind the target mAb in a crude plant extract and the elution buffer was optimized for high mAb recovery, product activity and affinity resin stability.
31475984	9	54	theme	capture	1659:1665	arg1	step					1667:1670	an initial capture step	1648:1670	an initial capture step	1648:1670	The new resins allow gentler elution conditions than Protein A and could also reduce the costs of an initial capture step for mAb production.
31475984	6	55	theme	important	1141:1149	arg1	concentration					1113:1125	the soluble ligand concentration	1094:1125	the soluble ligand concentration	1094:1125	The pH and buffer composition and the soluble ligand concentration were the most important parameters during the coupling procedure, which was improved using a design-of-experiments approach.
31475984	6	55	theme	important	1141:1149	arg1	parameters					1151:1160	the most important parameters	1132:1160	the most important parameters	1132:1160	The pH and buffer composition and the soluble ligand concentration were the most important parameters during the coupling procedure, which was improved using a design-of-experiments approach.
31475984	6	55	theme	important	1141:1149	arg1	pH					1064:1065	The pH	1060:1065	The pH	1060:1065	The pH and buffer composition and the soluble ligand concentration were the most important parameters during the coupling procedure, which was improved using a design-of-experiments approach.
31475984	6	55	theme	important	1141:1149	arg1	composition					1078:1088	buffer composition	1071:1088	buffer composition	1071:1088	The pH and buffer composition and the soluble ligand concentration were the most important parameters during the coupling procedure, which was improved using a design-of-experiments approach.
31475984	3	56	theme	ligand	524:529	arg1	immobilization					484:497	the immobilization	480:497	the immobilization of a DsRed-based epitope ligand to a cross-linked agarose resin allowing the selective capture of the HIV-neutralizing antibody 2F5 from crude plant extracts without using Protein A	480:679	Here we present a method for the immobilization of a DsRed-based epitope ligand to a cross-linked agarose resin allowing the selective capture of the HIV-neutralizing antibody 2F5 from crude plant extracts without using Protein A.
31475984	4	57	theme	affinity	900:907	arg1	chromatography					909:922	immobilized metal-ion affinity chromatography	878:922	immobilized metal-ion affinity chromatography	878:922	The linear epitope ELDKWA was first genetically fused to the fluorescent protein DsRed and the fusion protein was expressed in transgenic tobacco (Nicotiana tabacum) plants before purification by immobilized metal-ion affinity chromatography.
31475984	6	58	theme	design-of-experiments	1220:1240	arg1	approach					1242:1249	a design-of-experiments approach	1218:1249	a design-of-experiments approach	1218:1249	The pH and buffer composition and the soluble ligand concentration were the most important parameters during the coupling procedure, which was improved using a design-of-experiments approach.
31475984	3	59	theme	agarose	549:555	arg1	resin					557:561	a cross-linked agarose resin	534:561	a cross-linked agarose resin allowing the selective capture of the HIV-neutralizing antibody 2F5 from crude plant extracts without using Protein A	534:679	Here we present a method for the immobilization of a DsRed-based epitope ligand to a cross-linked agarose resin allowing the selective capture of the HIV-neutralizing antibody 2F5 from crude plant extracts without using Protein A.
31475984	4	60	theme	immobilized	878:888	arg1	chromatography					909:922	immobilized metal-ion affinity chromatography	878:922	immobilized metal-ion affinity chromatography	878:922	The linear epitope ELDKWA was first genetically fused to the fluorescent protein DsRed and the fusion protein was expressed in transgenic tobacco (Nicotiana tabacum) plants before purification by immobilized metal-ion affinity chromatography.
31475984	7	61	theme	plant	1354:1358	arg1	extract					1360:1366	a crude plant extract	1346:1366	a crude plant extract	1346:1366	The resulting affinity resin was tested for its ability to selectively bind the target mAb in a crude plant extract and the elution buffer was optimized for high mAb recovery, product activity and affinity resin stability.
31475984	4	62	theme	epitope	693:699	arg1	ELDKWA					701:706	The linear epitope ELDKWA	682:706	The linear epitope ELDKWA	682:706	The linear epitope ELDKWA was first genetically fused to the fluorescent protein DsRed and the fusion protein was expressed in transgenic tobacco (Nicotiana tabacum) plants before purification by immobilized metal-ion affinity chromatography.
31475984	0	63	theme	Chromatography	69:82	arg1	Resins					84:89	Affinity Chromatography Resins	60:89	Affinity Chromatography Resins	60:89	Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study.
31475984	3	64	theme	selective	576:584	arg1	capture					586:592	the selective capture	572:592	the selective capture of the HIV-neutralizing antibody 2F5 from crude plant extracts	572:655	Here we present a method for the immobilization of a DsRed-based epitope ligand to a cross-linked agarose resin allowing the selective capture of the HIV-neutralizing antibody 2F5 from crude plant extracts without using Protein A.
31475984	1	65	dep	25	263:264	arg1	to					260:261	to	260:261	to	260:261	The purification of monoclonal antibodies (mAbs) is commonly achieved by Protein A affinity chromatography, which can account for up to 25% of the overall process costs.
31475984	1	66	theme	monoclonal	147:156	arg1	mAbs					170:173	mAbs	170:173	mAbs	170:173	The purification of monoclonal antibodies (mAbs) is commonly achieved by Protein A affinity chromatography, which can account for up to 25% of the overall process costs.
31475984	1	66	theme	monoclonal	147:156	arg1	antibodies					158:167	monoclonal antibodies	147:167	monoclonal antibodies (mAbs)	147:174	The purification of monoclonal antibodies (mAbs) is commonly achieved by Protein A affinity chromatography, which can account for up to 25% of the overall process costs.
31475984	3	67	link	cross-linked	536:547	arg1	resin					557:561	a cross-linked agarose resin	534:561	a cross-linked agarose resin allowing the selective capture of the HIV-neutralizing antibody 2F5 from crude plant extracts without using Protein A	534:679	Here we present a method for the immobilization of a DsRed-based epitope ligand to a cross-linked agarose resin allowing the selective capture of the HIV-neutralizing antibody 2F5 from crude plant extracts without using Protein A.
31475984	5	68	theme	cross-linked	966:977	arg1	agarose					979:985	activated cross-linked agarose	956:985	activated cross-linked agarose	956:985	Furthermore, a method based on activated cross-linked agarose was optimized for high ligand density, efficient coupling and low costs.
31475984	7	69	theme	target	1332:1337	arg1	mAb					1339:1341	the target mAb	1328:1341	the target mAb	1328:1341	The resulting affinity resin was tested for its ability to selectively bind the target mAb in a crude plant extract and the elution buffer was optimized for high mAb recovery, product activity and affinity resin stability.
31475984	4	70	theme	fluorescent	743:753	arg1	DsRed					763:767	the fluorescent protein DsRed	739:767	the fluorescent protein DsRed	739:767	The linear epitope ELDKWA was first genetically fused to the fluorescent protein DsRed and the fusion protein was expressed in transgenic tobacco (Nicotiana tabacum) plants before purification by immobilized metal-ion affinity chromatography.
31475984	0	71	theme	Cross-linked	10:21	arg1	Agarose					23:29	Activated Cross-linked Agarose	0:29	Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study.	0:125	Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study.
31475984	8	72	theme	linear	1533:1538	arg1	epitopes					1540:1547	linear epitopes	1533:1547	linear epitopes	1533:1547	The method can easily be adapted to other antibodies with linear epitopes.
31475984	3	73	theme	2F5	627:629	arg1	capture					586:592	the selective capture	572:592	the selective capture of the HIV-neutralizing antibody 2F5 from crude plant extracts	572:655	Here we present a method for the immobilization of a DsRed-based epitope ligand to a cross-linked agarose resin allowing the selective capture of the HIV-neutralizing antibody 2F5 from crude plant extracts without using Protein A.
31475984	2	74	dep	Alternative	297:307	arg1	cost-effective					310:323	cost-effective	310:323	cost-effective	310:323	Alternative, cost-effective capture steps are therefore valuable for industrial-scale manufacturing, where large quantities of a single mAb are produced.
31475984	0	75	theme	Rapid	39:43	arg1	Development					45:55	the Rapid Development	35:55	the Rapid Development of Affinity Chromatography Resins	35:89	Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study.
31475984	9	76	theme	gentler	1571:1577	arg1	conditions					1587:1596	gentler elution conditions	1571:1596	gentler elution conditions	1571:1596	The new resins allow gentler elution conditions than Protein A and could also reduce the costs of an initial capture step for mAb production.
31475984	3	77	theme	HIV-neutralizing	601:616	arg1	2F5					627:629	the HIV-neutralizing antibody 2F5	597:629	the HIV-neutralizing antibody 2F5	597:629	Here we present a method for the immobilization of a DsRed-based epitope ligand to a cross-linked agarose resin allowing the selective capture of the HIV-neutralizing antibody 2F5 from crude plant extracts without using Protein A.
31475984	2	78	theme	mAb	433:435	arg1	quantities					410:419	large quantities	404:419	large quantities of a single mAb	404:435	Alternative, cost-effective capture steps are therefore valuable for industrial-scale manufacturing, where large quantities of a single mAb are produced.
31475984	2	79	theme	Alternative	297:307	arg1	steps					333:337	Alternative, cost-effective capture steps	297:337	Alternative, cost-effective capture steps	297:337	Alternative, cost-effective capture steps are therefore valuable for industrial-scale manufacturing, where large quantities of a single mAb are produced.
31475984	1	80	theme	process	282:288	arg1	costs					290:294	the overall process costs	270:294	the overall process costs	270:294	The purification of monoclonal antibodies (mAbs) is commonly achieved by Protein A affinity chromatography, which can account for up to 25% of the overall process costs.
31475984	9	81	theme	Protein	1603:1609	arg1	A					1611:1611	Protein A	1603:1611	Protein A	1603:1611	The new resins allow gentler elution conditions than Protein A and could also reduce the costs of an initial capture step for mAb production.
31475984	7	82	theme	elution	1376:1382	arg1	buffer					1384:1389	the elution buffer	1372:1389	the elution buffer	1372:1389	The resulting affinity resin was tested for its ability to selectively bind the target mAb in a crude plant extract and the elution buffer was optimized for high mAb recovery, product activity and affinity resin stability.
31475984	6	83	theme	ligand	1106:1111	arg1	pH					1064:1065	The pH	1060:1065	The pH	1060:1065	The pH and buffer composition and the soluble ligand concentration were the most important parameters during the coupling procedure, which was improved using a design-of-experiments approach.
31475984	6	83	theme	ligand	1106:1111	arg1	parameters					1151:1160	the most important parameters	1132:1160	the most important parameters	1132:1160	The pH and buffer composition and the soluble ligand concentration were the most important parameters during the coupling procedure, which was improved using a design-of-experiments approach.
31475984	6	83	theme	ligand	1106:1111	arg1	concentration					1113:1125	the soluble ligand concentration	1094:1125	the soluble ligand concentration	1094:1125	The pH and buffer composition and the soluble ligand concentration were the most important parameters during the coupling procedure, which was improved using a design-of-experiments approach.
31475984	6	83	theme	ligand	1106:1111	arg1	composition					1078:1088	buffer composition	1071:1088	buffer composition	1071:1088	The pH and buffer composition and the soluble ligand concentration were the most important parameters during the coupling procedure, which was improved using a design-of-experiments approach.
31475984	3	84	theme	crude	636:640	arg1	extracts					648:655	crude plant extracts	636:655	crude plant extracts	636:655	Here we present a method for the immobilization of a DsRed-based epitope ligand to a cross-linked agarose resin allowing the selective capture of the HIV-neutralizing antibody 2F5 from crude plant extracts without using Protein A.
31475984	1	85	theme	affinity	210:217	arg1	chromatography					219:232	Protein A affinity chromatography	200:232	Protein A affinity chromatography	200:232	The purification of monoclonal antibodies (mAbs) is commonly achieved by Protein A affinity chromatography, which can account for up to 25% of the overall process costs.
31475984	7	86	theme	resulting	1256:1264	arg1	resin					1275:1279	The resulting affinity resin	1252:1279	The resulting affinity resin	1252:1279	The resulting affinity resin was tested for its ability to selectively bind the target mAb in a crude plant extract and the elution buffer was optimized for high mAb recovery, product activity and affinity resin stability.
30986463	4	0	theme	Sutterella	613:622	arg1	proportions					566:576	the proportions	562:576	the proportions of Akkermansia, Rikenellaceae, and Sutterella	562:622	Taxonomic analysis indicated that the proportions of Akkermansia, Rikenellaceae, and Sutterella showed the greatest increase in the ceca of mice fed raw potato starch.
30986463	1	1	theme	human	117:121	arg1	health					123:128	human health	117:128	human health	117:128	The gut microbiome plays a pivotal role in human health and is affected by various factors.
30986463	6	2	theme	potato	883:888	arg1	group					897:901	The raw potato starch group	875:901	The raw potato starch group	875:901	The raw potato starch group also produced the highest propionic acid content.
30986463	6	3	theme	acid	939:942	arg1	content					944:950	the highest propionic acid content	917:950	the highest propionic acid content	917:950	The raw potato starch group also produced the highest propionic acid content.
30986463	5	4	theme	energy	795:800	arg1	metabolism					802:811	energy metabolism	795:811	energy metabolism	795:811	In addition, the gut microbiota of the raw potato starch group showed the highest carbohydrate and energy metabolism of all the groups, as confirmed by cecal metabolite analysis.
30986463	2	5	from	diet	281:284	arg1	gut					254:256	the gut	250:256	the gut	250:256	To investigate the association between phenotypic and microbiota-related changes in the gut and a raw starch-based diet, we fed mice with different starch substitutes (corn, wheat, rice, and potato) for 16 weeks.
30986463	6	6	theme	raw	879:881	arg1	group					897:901	The raw potato starch group	875:901	The raw potato starch group	875:901	The raw potato starch group also produced the highest propionic acid content.
30986463	7	7	theme	digestibility	1071:1083	arg1	terms					1062:1066	terms	1062:1066	terms of digestibility	1062:1083	Our results showed that the differences in the digestibility of each starch, differences in the phenotype in terms of digestibility, and changes in intestinal microbiota were connected, and it was confirmed that potato starch, which had the lowest digestibility, caused the greatest difference in intestinal microbe composition and metabolism.
30986463	0	8	from	Effect	0:5	arg1	gut					35:37	the gut	31:37	the gut	31:37	Effect of raw potato starch on the gut microbiome and metabolome in mice.
30986463	7	9	theme	greatest	1227:1234	arg1	difference					1236:1245	the greatest difference	1223:1245	the greatest difference in intestinal microbe composition and metabolism	1223:1294	Our results showed that the differences in the digestibility of each starch, differences in the phenotype in terms of digestibility, and changes in intestinal microbiota were connected, and it was confirmed that potato starch, which had the lowest digestibility, caused the greatest difference in intestinal microbe composition and metabolism.
30986463	3	10	theme	groups	476:481	arg1	gain					432:435	the lowest weight gain	414:435	the lowest weight gain	414:435	The potato starch-fed group showed the lowest weight gain and fat tissue accumulation of all the groups, as well as the highest insulin sensitivity.
30986463	3	10	theme	groups	476:481	arg1	accumulation					452:463	fat tissue accumulation	441:463	fat tissue accumulation	441:463	The potato starch-fed group showed the lowest weight gain and fat tissue accumulation of all the groups, as well as the highest insulin sensitivity.
30986463	3	10	theme	groups	476:481	arg1	sensitivity					515:525	the highest insulin sensitivity	495:525	the highest insulin sensitivity	495:525	The potato starch-fed group showed the lowest weight gain and fat tissue accumulation of all the groups, as well as the highest insulin sensitivity.
30986463	4	11	theme	mice	668:671	arg1	ceca					660:663	the ceca	656:663	the ceca of mice fed raw potato starch	656:693	Taxonomic analysis indicated that the proportions of Akkermansia, Rikenellaceae, and Sutterella showed the greatest increase in the ceca of mice fed raw potato starch.
30986463	3	12	theme	weight	425:430	arg1	gain					432:435	the lowest weight gain	414:435	the lowest weight gain	414:435	The potato starch-fed group showed the lowest weight gain and fat tissue accumulation of all the groups, as well as the highest insulin sensitivity.
30986463	4	13	theme	greatest	635:642	arg1	increase					644:651	the greatest increase	631:651	the greatest increase in the ceca of mice fed raw potato starch	631:693	Taxonomic analysis indicated that the proportions of Akkermansia, Rikenellaceae, and Sutterella showed the greatest increase in the ceca of mice fed raw potato starch.
30986463	7	14	from	differences	1030:1040	arg1	digestibility					1000:1012	the digestibility	996:1012	the digestibility of each starch	996:1027	Our results showed that the differences in the digestibility of each starch, differences in the phenotype in terms of digestibility, and changes in intestinal microbiota were connected, and it was confirmed that potato starch, which had the lowest digestibility, caused the greatest difference in intestinal microbe composition and metabolism.
30986463	7	14	from	differences	1030:1040	arg1	microbiota					1112:1121	intestinal microbiota	1101:1121	intestinal microbiota	1101:1121	Our results showed that the differences in the digestibility of each starch, differences in the phenotype in terms of digestibility, and changes in intestinal microbiota were connected, and it was confirmed that potato starch, which had the lowest digestibility, caused the greatest difference in intestinal microbe composition and metabolism.
30986463	7	14	from	differences	1030:1040	arg1	phenotype					1049:1057	the phenotype	1045:1057	the phenotype in terms of digestibility	1045:1083	Our results showed that the differences in the digestibility of each starch, differences in the phenotype in terms of digestibility, and changes in intestinal microbiota were connected, and it was confirmed that potato starch, which had the lowest digestibility, caused the greatest difference in intestinal microbe composition and metabolism.
30986463	7	15	theme	intestinal	1250:1259	arg1	composition					1269:1279	intestinal microbe composition	1250:1279	intestinal microbe composition	1250:1279	Our results showed that the differences in the digestibility of each starch, differences in the phenotype in terms of digestibility, and changes in intestinal microbiota were connected, and it was confirmed that potato starch, which had the lowest digestibility, caused the greatest difference in intestinal microbe composition and metabolism.
30986463	6	16	theme	propionic	929:937	arg1	content					944:950	the highest propionic acid content	917:950	the highest propionic acid content	917:950	The raw potato starch group also produced the highest propionic acid content.
30986463	3	17	theme	tissue	445:450	arg1	accumulation					452:463	fat tissue accumulation	441:463	fat tissue accumulation	441:463	The potato starch-fed group showed the lowest weight gain and fat tissue accumulation of all the groups, as well as the highest insulin sensitivity.
30986463	5	18	theme	starch	746:751	arg1	group					753:757	the raw potato starch group	731:757	the raw potato starch group	731:757	In addition, the gut microbiota of the raw potato starch group showed the highest carbohydrate and energy metabolism of all the groups, as confirmed by cecal metabolite analysis.
30986463	7	19	from	phenotype	1049:1057	arg1	terms					1062:1066	terms	1062:1066	terms of digestibility	1062:1083	Our results showed that the differences in the digestibility of each starch, differences in the phenotype in terms of digestibility, and changes in intestinal microbiota were connected, and it was confirmed that potato starch, which had the lowest digestibility, caused the greatest difference in intestinal microbe composition and metabolism.
30986463	2	20	dep	starch	314:319	arg1	rice					347:350	rice	347:350	rice	347:350	To investigate the association between phenotypic and microbiota-related changes in the gut and a raw starch-based diet, we fed mice with different starch substitutes (corn, wheat, rice, and potato) for 16 weeks.
30986463	2	20	dep	starch	314:319	arg1	potato					357:362	potato	357:362	potato	357:362	To investigate the association between phenotypic and microbiota-related changes in the gut and a raw starch-based diet, we fed mice with different starch substitutes (corn, wheat, rice, and potato) for 16 weeks.
30986463	2	20	dep	starch	314:319	arg1	wheat					340:344	wheat	340:344	wheat	340:344	To investigate the association between phenotypic and microbiota-related changes in the gut and a raw starch-based diet, we fed mice with different starch substitutes (corn, wheat, rice, and potato) for 16 weeks.
30986463	2	20	dep	starch	314:319	arg1	corn					334:337	corn	334:337	corn	334:337	To investigate the association between phenotypic and microbiota-related changes in the gut and a raw starch-based diet, we fed mice with different starch substitutes (corn, wheat, rice, and potato) for 16 weeks.
30986463	2	20	dep	starch	314:319	arg1	substitutes					321:331	substitutes	321:331	substitutes	321:331	To investigate the association between phenotypic and microbiota-related changes in the gut and a raw starch-based diet, we fed mice with different starch substitutes (corn, wheat, rice, and potato) for 16 weeks.
30986463	5	21	theme	group	753:757	arg1	microbiota					717:726	the gut microbiota	709:726	the gut microbiota of the raw potato starch group	709:757	In addition, the gut microbiota of the raw potato starch group showed the highest carbohydrate and energy metabolism of all the groups, as confirmed by cecal metabolite analysis.
30986463	5	22	theme	groups	824:829	arg1	carbohydrate					778:789	carbohydrate	778:789	carbohydrate	778:789	In addition, the gut microbiota of the raw potato starch group showed the highest carbohydrate and energy metabolism of all the groups, as confirmed by cecal metabolite analysis.
30986463	5	22	theme	groups	824:829	arg1	metabolism					802:811	energy metabolism	795:811	energy metabolism	795:811	In addition, the gut microbiota of the raw potato starch group showed the highest carbohydrate and energy metabolism of all the groups, as confirmed by cecal metabolite analysis.
30986463	2	23	theme	starch-based	268:279	arg1	diet					281:284	a raw starch-based diet	262:284	a raw starch-based diet	262:284	To investigate the association between phenotypic and microbiota-related changes in the gut and a raw starch-based diet, we fed mice with different starch substitutes (corn, wheat, rice, and potato) for 16 weeks.
30986463	4	24	theme	Akkermansia	581:591	arg1	proportions					566:576	the proportions	562:576	the proportions of Akkermansia, Rikenellaceae, and Sutterella	562:622	Taxonomic analysis indicated that the proportions of Akkermansia, Rikenellaceae, and Sutterella showed the greatest increase in the ceca of mice fed raw potato starch.
30986463	0	25	theme	potato	14:19	arg1	starch					21:26	raw potato starch	10:26	raw potato starch	10:26	Effect of raw potato starch on the gut microbiome and metabolome in mice.
30986463	2	26	theme	raw	264:266	arg1	diet					281:284	a raw starch-based diet	262:284	a raw starch-based diet	262:284	To investigate the association between phenotypic and microbiota-related changes in the gut and a raw starch-based diet, we fed mice with different starch substitutes (corn, wheat, rice, and potato) for 16 weeks.
30986463	7	27	from	difference	1236:1245	arg1	metabolism					1285:1294	metabolism	1285:1294	metabolism	1285:1294	Our results showed that the differences in the digestibility of each starch, differences in the phenotype in terms of digestibility, and changes in intestinal microbiota were connected, and it was confirmed that potato starch, which had the lowest digestibility, caused the greatest difference in intestinal microbe composition and metabolism.
30986463	7	27	from	difference	1236:1245	arg1	composition					1269:1279	intestinal microbe composition	1250:1279	intestinal microbe composition	1250:1279	Our results showed that the differences in the digestibility of each starch, differences in the phenotype in terms of digestibility, and changes in intestinal microbiota were connected, and it was confirmed that potato starch, which had the lowest digestibility, caused the greatest difference in intestinal microbe composition and metabolism.
30986463	1	28	theme	various	149:155	arg1	factors					157:163	various factors	149:163	various factors	149:163	The gut microbiome plays a pivotal role in human health and is affected by various factors.
30986463	0	29	theme	raw	10:12	arg1	starch					21:26	raw potato starch	10:26	raw potato starch	10:26	Effect of raw potato starch on the gut microbiome and metabolome in mice.
30986463	4	30	theme	potato	681:686	arg1	starch					688:693	raw potato starch	677:693	raw potato starch	677:693	Taxonomic analysis indicated that the proportions of Akkermansia, Rikenellaceae, and Sutterella showed the greatest increase in the ceca of mice fed raw potato starch.
30986463	1	31	theme	gut	78:80	arg1	microbiome					82:91	The gut microbiome	74:91	The gut microbiome	74:91	The gut microbiome plays a pivotal role in human health and is affected by various factors.
30986463	2	32	from	changes	239:245	arg1	gut					254:256	the gut	250:256	the gut	250:256	To investigate the association between phenotypic and microbiota-related changes in the gut and a raw starch-based diet, we fed mice with different starch substitutes (corn, wheat, rice, and potato) for 16 weeks.
30986463	3	33	theme	starch-fed	390:399	arg1	group					401:405	The potato starch-fed group	379:405	The potato starch-fed group	379:405	The potato starch-fed group showed the lowest weight gain and fat tissue accumulation of all the groups, as well as the highest insulin sensitivity.
30986463	7	34	theme	potato	1165:1170	arg1	starch					1172:1177	potato starch	1165:1177	potato starch	1165:1177	Our results showed that the differences in the digestibility of each starch, differences in the phenotype in terms of digestibility, and changes in intestinal microbiota were connected, and it was confirmed that potato starch, which had the lowest digestibility, caused the greatest difference in intestinal microbe composition and metabolism.
30986463	7	35	from	differences	981:991	arg1	digestibility					1000:1012	the digestibility	996:1012	the digestibility of each starch	996:1027	Our results showed that the differences in the digestibility of each starch, differences in the phenotype in terms of digestibility, and changes in intestinal microbiota were connected, and it was confirmed that potato starch, which had the lowest digestibility, caused the greatest difference in intestinal microbe composition and metabolism.
30986463	7	35	from	differences	981:991	arg1	microbiota					1112:1121	intestinal microbiota	1101:1121	intestinal microbiota	1101:1121	Our results showed that the differences in the digestibility of each starch, differences in the phenotype in terms of digestibility, and changes in intestinal microbiota were connected, and it was confirmed that potato starch, which had the lowest digestibility, caused the greatest difference in intestinal microbe composition and metabolism.
30986463	7	35	from	differences	981:991	arg1	phenotype					1049:1057	the phenotype	1045:1057	the phenotype in terms of digestibility	1045:1083	Our results showed that the differences in the digestibility of each starch, differences in the phenotype in terms of digestibility, and changes in intestinal microbiota were connected, and it was confirmed that potato starch, which had the lowest digestibility, caused the greatest difference in intestinal microbe composition and metabolism.
30986463	6	36	theme	highest	921:927	arg1	content					944:950	the highest propionic acid content	917:950	the highest propionic acid content	917:950	The raw potato starch group also produced the highest propionic acid content.
30986463	5	37	theme	raw	735:737	arg1	group					753:757	the raw potato starch group	731:757	the raw potato starch group	731:757	In addition, the gut microbiota of the raw potato starch group showed the highest carbohydrate and energy metabolism of all the groups, as confirmed by cecal metabolite analysis.
30986463	0	38	theme	starch	21:26	arg1	Effect					0:5	Effect	0:5	Effect of raw potato starch on the gut	0:37	Effect of raw potato starch on the gut microbiome and metabolome in mice.
30986463	2	39	with	mice	294:297	arg1	starch					314:319	starch	314:319	starch	314:319	To investigate the association between phenotypic and microbiota-related changes in the gut and a raw starch-based diet, we fed mice with different starch substitutes (corn, wheat, rice, and potato) for 16 weeks.
30986463	3	40	theme	highest	499:505	arg1	sensitivity					515:525	the highest insulin sensitivity	495:525	the highest insulin sensitivity	495:525	The potato starch-fed group showed the lowest weight gain and fat tissue accumulation of all the groups, as well as the highest insulin sensitivity.
30986463	5	41	theme	potato	739:744	arg1	group					753:757	the raw potato starch group	731:757	the raw potato starch group	731:757	In addition, the gut microbiota of the raw potato starch group showed the highest carbohydrate and energy metabolism of all the groups, as confirmed by cecal metabolite analysis.
30986463	5	42	theme	gut	713:715	arg1	microbiota					717:726	the gut microbiota	709:726	the gut microbiota of the raw potato starch group	709:757	In addition, the gut microbiota of the raw potato starch group showed the highest carbohydrate and energy metabolism of all the groups, as confirmed by cecal metabolite analysis.
30986463	5	43	theme	cecal	848:852	arg1	analysis					865:872	cecal metabolite analysis	848:872	cecal metabolite analysis	848:872	In addition, the gut microbiota of the raw potato starch group showed the highest carbohydrate and energy metabolism of all the groups, as confirmed by cecal metabolite analysis.
30986463	7	44	theme	starch	1022:1027	arg1	digestibility					1000:1012	the digestibility	996:1012	the digestibility of each starch	996:1027	Our results showed that the differences in the digestibility of each starch, differences in the phenotype in terms of digestibility, and changes in intestinal microbiota were connected, and it was confirmed that potato starch, which had the lowest digestibility, caused the greatest difference in intestinal microbe composition and metabolism.
30986463	7	45	contain	had	1186:1188	arg1	starch					1172:1177	potato starch	1165:1177	potato starch	1165:1177	Our results showed that the differences in the digestibility of each starch, differences in the phenotype in terms of digestibility, and changes in intestinal microbiota were connected, and it was confirmed that potato starch, which had the lowest digestibility, caused the greatest difference in intestinal microbe composition and metabolism.
30986463	7	45	contain	had	1186:1188	arg2	digestibility					1201:1213	the lowest digestibility	1190:1213	the lowest digestibility	1190:1213	Our results showed that the differences in the digestibility of each starch, differences in the phenotype in terms of digestibility, and changes in intestinal microbiota were connected, and it was confirmed that potato starch, which had the lowest digestibility, caused the greatest difference in intestinal microbe composition and metabolism.
30986463	3	46	theme	lowest	418:423	arg1	gain					432:435	the lowest weight gain	414:435	the lowest weight gain	414:435	The potato starch-fed group showed the lowest weight gain and fat tissue accumulation of all the groups, as well as the highest insulin sensitivity.
30986463	4	47	from	increase	644:651	arg1	ceca					660:663	the ceca	656:663	the ceca of mice fed raw potato starch	656:693	Taxonomic analysis indicated that the proportions of Akkermansia, Rikenellaceae, and Sutterella showed the greatest increase in the ceca of mice fed raw potato starch.
30986463	7	48	from	changes	1090:1096	arg1	digestibility					1000:1012	the digestibility	996:1012	the digestibility of each starch	996:1027	Our results showed that the differences in the digestibility of each starch, differences in the phenotype in terms of digestibility, and changes in intestinal microbiota were connected, and it was confirmed that potato starch, which had the lowest digestibility, caused the greatest difference in intestinal microbe composition and metabolism.
30986463	7	48	from	changes	1090:1096	arg1	microbiota					1112:1121	intestinal microbiota	1101:1121	intestinal microbiota	1101:1121	Our results showed that the differences in the digestibility of each starch, differences in the phenotype in terms of digestibility, and changes in intestinal microbiota were connected, and it was confirmed that potato starch, which had the lowest digestibility, caused the greatest difference in intestinal microbe composition and metabolism.
30986463	7	48	from	changes	1090:1096	arg1	phenotype					1049:1057	the phenotype	1045:1057	the phenotype in terms of digestibility	1045:1083	Our results showed that the differences in the digestibility of each starch, differences in the phenotype in terms of digestibility, and changes in intestinal microbiota were connected, and it was confirmed that potato starch, which had the lowest digestibility, caused the greatest difference in intestinal microbe composition and metabolism.
30986463	4	49	theme	Rikenellaceae	594:606	arg1	proportions					566:576	the proportions	562:576	the proportions of Akkermansia, Rikenellaceae, and Sutterella	562:622	Taxonomic analysis indicated that the proportions of Akkermansia, Rikenellaceae, and Sutterella showed the greatest increase in the ceca of mice fed raw potato starch.
30986463	7	50	theme	microbe	1261:1267	arg1	composition					1269:1279	intestinal microbe composition	1250:1279	intestinal microbe composition	1250:1279	Our results showed that the differences in the digestibility of each starch, differences in the phenotype in terms of digestibility, and changes in intestinal microbiota were connected, and it was confirmed that potato starch, which had the lowest digestibility, caused the greatest difference in intestinal microbe composition and metabolism.
30986463	4	51	theme	raw	677:679	arg1	starch					688:693	raw potato starch	677:693	raw potato starch	677:693	Taxonomic analysis indicated that the proportions of Akkermansia, Rikenellaceae, and Sutterella showed the greatest increase in the ceca of mice fed raw potato starch.
30986463	2	52	theme	microbiota-related	220:237	arg1	changes					239:245	phenotypic and microbiota-related changes	205:245	phenotypic and microbiota-related changes in the gut	205:256	To investigate the association between phenotypic and microbiota-related changes in the gut and a raw starch-based diet, we fed mice with different starch substitutes (corn, wheat, rice, and potato) for 16 weeks.
30986463	1	53	theme	pivotal	101:107	arg1	role					109:112	a pivotal role	99:112	a pivotal role	99:112	The gut microbiome plays a pivotal role in human health and is affected by various factors.
30986463	5	54	theme	metabolite	854:863	arg1	analysis					865:872	cecal metabolite analysis	848:872	cecal metabolite analysis	848:872	In addition, the gut microbiota of the raw potato starch group showed the highest carbohydrate and energy metabolism of all the groups, as confirmed by cecal metabolite analysis.
30986463	3	55	theme	insulin	507:513	arg1	sensitivity					515:525	the highest insulin sensitivity	495:525	the highest insulin sensitivity	495:525	The potato starch-fed group showed the lowest weight gain and fat tissue accumulation of all the groups, as well as the highest insulin sensitivity.
30986463	3	56	theme	potato	383:388	arg1	group					401:405	The potato starch-fed group	379:405	The potato starch-fed group	379:405	The potato starch-fed group showed the lowest weight gain and fat tissue accumulation of all the groups, as well as the highest insulin sensitivity.
30986463	5	57	theme	highest	770:776	arg1	carbohydrate					778:789	carbohydrate	778:789	carbohydrate	778:789	In addition, the gut microbiota of the raw potato starch group showed the highest carbohydrate and energy metabolism of all the groups, as confirmed by cecal metabolite analysis.
30986463	7	58	theme	lowest	1194:1199	arg1	digestibility					1201:1213	the lowest digestibility	1190:1213	the lowest digestibility	1190:1213	Our results showed that the differences in the digestibility of each starch, differences in the phenotype in terms of digestibility, and changes in intestinal microbiota were connected, and it was confirmed that potato starch, which had the lowest digestibility, caused the greatest difference in intestinal microbe composition and metabolism.
30986463	4	59	theme	Taxonomic	528:536	arg1	analysis					538:545	Taxonomic analysis	528:545	Taxonomic analysis	528:545	Taxonomic analysis indicated that the proportions of Akkermansia, Rikenellaceae, and Sutterella showed the greatest increase in the ceca of mice fed raw potato starch.
30986463	7	60	theme	intestinal	1101:1110	arg1	microbiota					1112:1121	intestinal microbiota	1101:1121	intestinal microbiota	1101:1121	Our results showed that the differences in the digestibility of each starch, differences in the phenotype in terms of digestibility, and changes in intestinal microbiota were connected, and it was confirmed that potato starch, which had the lowest digestibility, caused the greatest difference in intestinal microbe composition and metabolism.
30986463	2	61	theme	phenotypic	205:214	arg1	changes					239:245	phenotypic and microbiota-related changes	205:245	phenotypic and microbiota-related changes in the gut	205:256	To investigate the association between phenotypic and microbiota-related changes in the gut and a raw starch-based diet, we fed mice with different starch substitutes (corn, wheat, rice, and potato) for 16 weeks.
30986463	3	62	theme	fat	441:443	arg1	accumulation					452:463	fat tissue accumulation	441:463	fat tissue accumulation	441:463	The potato starch-fed group showed the lowest weight gain and fat tissue accumulation of all the groups, as well as the highest insulin sensitivity.
30986463	6	63	theme	starch	890:895	arg1	group					897:901	The raw potato starch group	875:901	The raw potato starch group	875:901	The raw potato starch group also produced the highest propionic acid content.
31796989	2	0	theme	aerobic	97:103	arg1	actinobacterium					112:126	A novel aerobic marine actinobacterium	89:126	A novel aerobic marine actinobacterium (strain S5-52T) belonging to the genus Glutamicibacter	89:181	A novel aerobic marine actinobacterium (strain S5-52T) belonging to the genus Glutamicibacter was isolated from the coral Favia veroni sampled from the Andaman Sea, India.
31796989	2	0	theme	aerobic	97:103	arg1	S5-52T					136:141	strain S5-52T	129:141	strain S5-52T	129:141	A novel aerobic marine actinobacterium (strain S5-52T) belonging to the genus Glutamicibacter was isolated from the coral Favia veroni sampled from the Andaman Sea, India.
31796989	6	1	theme	polar	385:389	arg1	diphosphatidylglycerol					403:424	diphosphatidylglycerol	403:424	diphosphatidylglycerol	403:424	The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, glycolipid, trimannosyldiacylglycerol, phospholipid and dimannosylglyceride.
31796989	6	1	theme	polar	385:389	arg1	lipids					391:396	The polar lipids	381:396	The polar lipids	381:396	The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, glycolipid, trimannosyldiacylglycerol, phospholipid and dimannosylglyceride.
31796989	11	2	dep	replication	1127:1137	arg1	mechanism					1165:1173	mechanism	1165:1173	mechanism	1165:1173	Analysis of genomic reduction events in the evolutionary path from the LUCA (last universal common ancestor) to G. mishrai LMG 29155T and G. halophytocola DSM 101718T exhibit a number of genes involved in amino acid metabolism, cell wall biogenesis and replication, recombination and repair mechanism that reduced in both the species.
31796989	11	3	theme	reduction	894:902	arg1	events					904:909	genomic reduction events	886:909	genomic reduction events in the evolutionary path from the LUCA (last universal common ancestor) to G. mishrai LMG 29155T and G. halophytocola DSM 101718T	886:1039	Analysis of genomic reduction events in the evolutionary path from the LUCA (last universal common ancestor) to G. mishrai LMG 29155T and G. halophytocola DSM 101718T exhibit a number of genes involved in amino acid metabolism, cell wall biogenesis and replication, recombination and repair mechanism that reduced in both the species.
31796989	8	4	theme	Glutamicibacter	624:638	arg1	101718T					658:664	Glutamicibacter halophytocola DSM 101718T	624:664	Glutamicibacter halophytocola DSM 101718T	624:664	Strain S5-52T showed a maximum 16S rRNA similarity of 99.36% with Glutamicibacter halophytocola DSM 101718T.
31796989	2	5	theme	novel	91:95	arg1	actinobacterium					112:126	A novel aerobic marine actinobacterium	89:126	A novel aerobic marine actinobacterium (strain S5-52T) belonging to the genus Glutamicibacter	89:181	A novel aerobic marine actinobacterium (strain S5-52T) belonging to the genus Glutamicibacter was isolated from the coral Favia veroni sampled from the Andaman Sea, India.
31796989	2	5	theme	novel	91:95	arg1	S5-52T					136:141	strain S5-52T	129:141	strain S5-52T	129:141	A novel aerobic marine actinobacterium (strain S5-52T) belonging to the genus Glutamicibacter was isolated from the coral Favia veroni sampled from the Andaman Sea, India.
31796989	12	6	theme	phenotypic	1218:1227	arg1	properties					1245:1254	phenotypic, chemotaxonomic properties	1218:1254	properties	1245:1254	Based on phenotypic, chemotaxonomic properties and comparative genomic studies, the strain S5-52T is considered a novel species of the genus Glutamicibacter, for which the name Glutamicibacter mishrai sp.
31796989	11	7	theme	events	904:909	arg1	Analysis					874:881	Analysis	874:881	Analysis of genomic reduction events in the evolutionary path from the LUCA (last universal common ancestor) to G. mishrai LMG 29155T and G. halophytocola DSM 101718T	874:1039	Analysis of genomic reduction events in the evolutionary path from the LUCA (last universal common ancestor) to G. mishrai LMG 29155T and G. halophytocola DSM 101718T exhibit a number of genes involved in amino acid metabolism, cell wall biogenesis and replication, recombination and repair mechanism that reduced in both the species.
31796989	8	8	theme	DSM	654:656	arg1	101718T					658:664	Glutamicibacter halophytocola DSM 101718T	624:664	Glutamicibacter halophytocola DSM 101718T	624:664	Strain S5-52T showed a maximum 16S rRNA similarity of 99.36% with Glutamicibacter halophytocola DSM 101718T.
31796989	11	9	from	LUCA	945:948	arg1	path					931:934	the evolutionary path	914:934	the evolutionary path from the LUCA (last universal common ancestor) to G. mishrai LMG 29155T and G. halophytocola DSM 101718T	914:1039	Analysis of genomic reduction events in the evolutionary path from the LUCA (last universal common ancestor) to G. mishrai LMG 29155T and G. halophytocola DSM 101718T exhibit a number of genes involved in amino acid metabolism, cell wall biogenesis and replication, recombination and repair mechanism that reduced in both the species.
31796989	11	9	from	LUCA	945:948	arg1	events					904:909	genomic reduction events	886:909	genomic reduction events in the evolutionary path from the LUCA (last universal common ancestor) to G. mishrai LMG 29155T and G. halophytocola DSM 101718T	886:1039	Analysis of genomic reduction events in the evolutionary path from the LUCA (last universal common ancestor) to G. mishrai LMG 29155T and G. halophytocola DSM 101718T exhibit a number of genes involved in amino acid metabolism, cell wall biogenesis and replication, recombination and repair mechanism that reduced in both the species.
31796989	14	10	theme	39846T = LMG	1466:1477	arg1	S5-52T					1451:1456	S5-52T	1451:1456	S5-52T (= KCTC 39846T = LMG 29155T)	1451:1485	The type strain is S5-52T (= KCTC 39846T = LMG 29155T).
31796989	14	10	theme	39846T = LMG	1466:1477	arg1	29155T					1479:1484	= KCTC 39846T = LMG 29155T	1459:1484	= KCTC 39846T = LMG 29155T	1459:1484	The type strain is S5-52T (= KCTC 39846T = LMG 29155T).
31796989	9	11	theme	strain	681:686	arg1	S5-52T					688:693	strain S5-52T	681:693	strain S5-52T	681:693	The genome of strain S5-52T was 3.57 Mb that contains 3274 protein coding sequences (CDS).
31796989	12	12	theme	Glutamicibacter	1350:1364	arg1	species					1329:1335	a novel species	1321:1335	a novel species	1321:1335	Based on phenotypic, chemotaxonomic properties and comparative genomic studies, the strain S5-52T is considered a novel species of the genus Glutamicibacter, for which the name Glutamicibacter mishrai sp.
31796989	9	13	theme	S5-52T	688:693	arg1	3.57 Mb					699:705	3.57 Mb	699:705	3.57 Mb that contains 3274 protein coding sequences (CDS)	699:755	The genome of strain S5-52T was 3.57 Mb that contains 3274 protein coding sequences (CDS).
31796989	9	13	theme	S5-52T	688:693	arg1	genome					671:676	The genome	667:676	The genome of strain S5-52T	667:693	The genome of strain S5-52T was 3.57 Mb that contains 3274 protein coding sequences (CDS).
31796989	14	14	theme	= KCTC	1459:1464	arg1	S5-52T					1451:1456	S5-52T	1451:1456	S5-52T (= KCTC 39846T = LMG 29155T)	1451:1485	The type strain is S5-52T (= KCTC 39846T = LMG 29155T).
31796989	14	14	theme	= KCTC	1459:1464	arg1	29155T					1479:1484	= KCTC 39846T = LMG 29155T	1459:1484	= KCTC 39846T = LMG 29155T	1459:1484	The type strain is S5-52T (= KCTC 39846T = LMG 29155T).
31796989	1	15	theme	coral	52:56	arg1	veroni					64:69	the coral Favia veroni	48:69	the coral Favia veroni from Andaman Sea	48:86	nov., isolated from the coral Favia veroni from Andaman Sea.
31796989	11	16	theme	genomic	886:892	arg1	events					904:909	genomic reduction events	886:909	genomic reduction events in the evolutionary path from the LUCA (last universal common ancestor) to G. mishrai LMG 29155T and G. halophytocola DSM 101718T	886:1039	Analysis of genomic reduction events in the evolutionary path from the LUCA (last universal common ancestor) to G. mishrai LMG 29155T and G. halophytocola DSM 101718T exhibit a number of genes involved in amino acid metabolism, cell wall biogenesis and replication, recombination and repair mechanism that reduced in both the species.
31796989	9	17	contain	contains	712:719	arg2	CDS					752:754	CDS	752:754	CDS	752:754	The genome of strain S5-52T was 3.57 Mb that contains 3274 protein coding sequences (CDS).
31796989	9	17	contain	contains	712:719	arg1	3.57 Mb					699:705	3.57 Mb	699:705	3.57 Mb that contains 3274 protein coding sequences (CDS)	699:755	The genome of strain S5-52T was 3.57 Mb that contains 3274 protein coding sequences (CDS).
31796989	9	17	contain	contains	712:719	arg1	genome					671:676	The genome	667:676	The genome of strain S5-52T	667:693	The genome of strain S5-52T was 3.57 Mb that contains 3274 protein coding sequences (CDS).
31796989	9	17	contain	contains	712:719	arg2	sequences					741:749	3274 protein coding sequences	721:749	3274 protein coding sequences (CDS)	721:755	The genome of strain S5-52T was 3.57 Mb that contains 3274 protein coding sequences (CDS).
31796989	2	18	theme	strain	129:134	arg1	actinobacterium					112:126	A novel aerobic marine actinobacterium	89:126	A novel aerobic marine actinobacterium (strain S5-52T) belonging to the genus Glutamicibacter	89:181	A novel aerobic marine actinobacterium (strain S5-52T) belonging to the genus Glutamicibacter was isolated from the coral Favia veroni sampled from the Andaman Sea, India.
31796989	2	18	theme	strain	129:134	arg1	S5-52T					136:141	strain S5-52T	129:141	strain S5-52T	129:141	A novel aerobic marine actinobacterium (strain S5-52T) belonging to the genus Glutamicibacter was isolated from the coral Favia veroni sampled from the Andaman Sea, India.
31796989	1	19	theme	Favia	58:62	arg1	veroni					64:69	the coral Favia veroni	48:69	the coral Favia veroni from Andaman Sea	48:86	nov., isolated from the coral Favia veroni from Andaman Sea.
31796989	12	20	theme	chemotaxonomic	1230:1243	arg1	properties					1245:1254	phenotypic, chemotaxonomic properties	1218:1254	properties	1245:1254	Based on phenotypic, chemotaxonomic properties and comparative genomic studies, the strain S5-52T is considered a novel species of the genus Glutamicibacter, for which the name Glutamicibacter mishrai sp.
31796989	11	21	theme	wall	1107:1110	arg1	biogenesis					1112:1121	cell wall biogenesis	1102:1121	cell wall biogenesis	1102:1121	Analysis of genomic reduction events in the evolutionary path from the LUCA (last universal common ancestor) to G. mishrai LMG 29155T and G. halophytocola DSM 101718T exhibit a number of genes involved in amino acid metabolism, cell wall biogenesis and replication, recombination and repair mechanism that reduced in both the species.
31796989	2	22	attach	isolated	187:194	arg2	S5-52T					136:141	strain S5-52T	129:141	strain S5-52T	129:141	A novel aerobic marine actinobacterium (strain S5-52T) belonging to the genus Glutamicibacter was isolated from the coral Favia veroni sampled from the Andaman Sea, India.
31796989	2	22	attach	isolated	187:194	arg2	actinobacterium					112:126	A novel aerobic marine actinobacterium	89:126	A novel aerobic marine actinobacterium (strain S5-52T) belonging to the genus Glutamicibacter	89:181	A novel aerobic marine actinobacterium (strain S5-52T) belonging to the genus Glutamicibacter was isolated from the coral Favia veroni sampled from the Andaman Sea, India.
31796989	2	22	attach	isolated	187:194	arg1	veroni					217:222	the coral Favia veroni	201:222	the coral Favia veroni sampled from the Andaman Sea, India	201:258	A novel aerobic marine actinobacterium (strain S5-52T) belonging to the genus Glutamicibacter was isolated from the coral Favia veroni sampled from the Andaman Sea, India.
31796989	0	23	theme	mishrai	16:22	arg1	sp					24:25	Glutamicibacter mishrai sp	0:25	Glutamicibacter mishrai sp.	0:26	Glutamicibacter mishrai sp.
31796989	4	24	theme	G+C	315:317	arg1	content					319:325	The DNA G+C content	307:325	The DNA G+C content	307:325	The DNA G+C content was 58.7 mol%.
31796989	4	24	theme	G+C	315:317	arg1	%					339:339	58.7 mol%	331:339	58.7 mol%	331:339	The DNA G+C content was 58.7 mol%.
31796989	12	25	theme	genus	1344:1348	arg1	Glutamicibacter					1350:1364	the genus Glutamicibacter	1340:1364	the genus Glutamicibacter	1340:1364	Based on phenotypic, chemotaxonomic properties and comparative genomic studies, the strain S5-52T is considered a novel species of the genus Glutamicibacter, for which the name Glutamicibacter mishrai sp.
31796989	10	26	theme	ANI	781:783	arg1	values					785:790	DNA-DNA similarity and ANI values	758:790	values	785:790	DNA-DNA similarity and ANI values between S5-52T and the reference strains were below 70% and 95-96%, respectively.
31796989	0	27	theme	Glutamicibacter	0:14	arg1	sp					24:25	Glutamicibacter mishrai sp	0:25	Glutamicibacter mishrai sp.	0:26	Glutamicibacter mishrai sp.
31796989	11	28	theme	evolutionary	918:929	arg1	path					931:934	the evolutionary path	914:934	the evolutionary path from the LUCA (last universal common ancestor) to G. mishrai LMG 29155T and G. halophytocola DSM 101718T	914:1039	Analysis of genomic reduction events in the evolutionary path from the LUCA (last universal common ancestor) to G. mishrai LMG 29155T and G. halophytocola DSM 101718T exhibit a number of genes involved in amino acid metabolism, cell wall biogenesis and replication, recombination and repair mechanism that reduced in both the species.
31796989	8	29	theme	%	617:617	arg1	similarity					598:607	a maximum 16S rRNA similarity	579:607	a maximum 16S rRNA similarity of 99.36% with Glutamicibacter halophytocola DSM 101718T	579:664	Strain S5-52T showed a maximum 16S rRNA similarity of 99.36% with Glutamicibacter halophytocola DSM 101718T.
31796989	4	30	theme	58.7 mol	331:338	arg1	content					319:325	The DNA G+C content	307:325	The DNA G+C content	307:325	The DNA G+C content was 58.7 mol%.
31796989	4	30	theme	58.7 mol	331:338	arg1	%					339:339	58.7 mol%	331:339	58.7 mol%	331:339	The DNA G+C content was 58.7 mol%.
31796989	1	31	theme	Andaman	76:82	arg1	Sea					84:86	Andaman Sea	76:86	Andaman Sea	76:86	nov., isolated from the coral Favia veroni from Andaman Sea.
31796989	14	32	theme	type	1436:1439	arg1	S5-52T					1451:1456	S5-52T	1451:1456	S5-52T (= KCTC 39846T = LMG 29155T)	1451:1485	The type strain is S5-52T (= KCTC 39846T = LMG 29155T).
31796989	14	32	theme	type	1436:1439	arg1	strain					1441:1446	The type strain	1432:1446	The type strain	1432:1446	The type strain is S5-52T (= KCTC 39846T = LMG 29155T).
31796989	9	33	theme	coding	734:739	arg1	CDS					752:754	CDS	752:754	CDS	752:754	The genome of strain S5-52T was 3.57 Mb that contains 3274 protein coding sequences (CDS).
31796989	9	33	theme	coding	734:739	arg1	sequences					741:749	3274 protein coding sequences	721:749	3274 protein coding sequences (CDS)	721:755	The genome of strain S5-52T was 3.57 Mb that contains 3274 protein coding sequences (CDS).
31796989	10	34	theme	reference	815:823	arg1	strains					825:831	the reference strains	811:831	the reference strains	811:831	DNA-DNA similarity and ANI values between S5-52T and the reference strains were below 70% and 95-96%, respectively.
31796989	8	35	theme	16S	589:591	arg1	similarity					598:607	a maximum 16S rRNA similarity	579:607	a maximum 16S rRNA similarity of 99.36% with Glutamicibacter halophytocola DSM 101718T	579:664	Strain S5-52T showed a maximum 16S rRNA similarity of 99.36% with Glutamicibacter halophytocola DSM 101718T.
31796989	9	36	theme	protein	726:732	arg1	CDS					752:754	CDS	752:754	CDS	752:754	The genome of strain S5-52T was 3.57 Mb that contains 3274 protein coding sequences (CDS).
31796989	9	36	theme	protein	726:732	arg1	sequences					741:749	3274 protein coding sequences	721:749	3274 protein coding sequences (CDS)	721:755	The genome of strain S5-52T was 3.57 Mb that contains 3274 protein coding sequences (CDS).
31796989	8	37	theme	maximum	581:587	arg1	similarity					598:607	a maximum 16S rRNA similarity	579:607	a maximum 16S rRNA similarity of 99.36% with Glutamicibacter halophytocola DSM 101718T	579:664	Strain S5-52T showed a maximum 16S rRNA similarity of 99.36% with Glutamicibacter halophytocola DSM 101718T.
31796989	2	38	theme	Favia	211:215	arg1	veroni					217:222	the coral Favia veroni	201:222	the coral Favia veroni sampled from the Andaman Sea, India	201:258	A novel aerobic marine actinobacterium (strain S5-52T) belonging to the genus Glutamicibacter was isolated from the coral Favia veroni sampled from the Andaman Sea, India.
31796989	12	39	theme	novel	1323:1327	arg1	species					1329:1335	a novel species	1321:1335	a novel species	1321:1335	Based on phenotypic, chemotaxonomic properties and comparative genomic studies, the strain S5-52T is considered a novel species of the genus Glutamicibacter, for which the name Glutamicibacter mishrai sp.
31796989	10	40	theme	DNA-DNA	758:764	arg1	similarity					766:775	DNA-DNA similarity and ANI values	758:790	similarity	766:775	DNA-DNA similarity and ANI values between S5-52T and the reference strains were below 70% and 95-96%, respectively.
31796989	12	41	theme	Glutamicibacter	1386:1400	arg1	sp					1410:1411	the name Glutamicibacter mishrai sp	1377:1411	the name Glutamicibacter mishrai sp	1377:1411	Based on phenotypic, chemotaxonomic properties and comparative genomic studies, the strain S5-52T is considered a novel species of the genus Glutamicibacter, for which the name Glutamicibacter mishrai sp.
31796989	11	42	from	events	904:909	arg1	path					931:934	the evolutionary path	914:934	the evolutionary path from the LUCA (last universal common ancestor) to G. mishrai LMG 29155T and G. halophytocola DSM 101718T	914:1039	Analysis of genomic reduction events in the evolutionary path from the LUCA (last universal common ancestor) to G. mishrai LMG 29155T and G. halophytocola DSM 101718T exhibit a number of genes involved in amino acid metabolism, cell wall biogenesis and replication, recombination and repair mechanism that reduced in both the species.
31796989	11	42	from	events	904:909	arg1	LUCA					945:948	the LUCA	941:948	the LUCA (last universal common ancestor)	941:981	Analysis of genomic reduction events in the evolutionary path from the LUCA (last universal common ancestor) to G. mishrai LMG 29155T and G. halophytocola DSM 101718T exhibit a number of genes involved in amino acid metabolism, cell wall biogenesis and replication, recombination and repair mechanism that reduced in both the species.
31796989	11	42	from	events	904:909	arg1	ancestor					973:980	last universal common ancestor	951:980	last universal common ancestor	951:980	Analysis of genomic reduction events in the evolutionary path from the LUCA (last universal common ancestor) to G. mishrai LMG 29155T and G. halophytocola DSM 101718T exhibit a number of genes involved in amino acid metabolism, cell wall biogenesis and replication, recombination and repair mechanism that reduced in both the species.
31796989	2	43	theme	coral	205:209	arg1	veroni					217:222	the coral Favia veroni	201:222	the coral Favia veroni sampled from the Andaman Sea, India	201:258	A novel aerobic marine actinobacterium (strain S5-52T) belonging to the genus Glutamicibacter was isolated from the coral Favia veroni sampled from the Andaman Sea, India.
31796989	11	44	theme	DSM	1029:1031	arg1	101718T					1033:1039	DSM 101718T	1029:1039	DSM 101718T	1029:1039	Analysis of genomic reduction events in the evolutionary path from the LUCA (last universal common ancestor) to G. mishrai LMG 29155T and G. halophytocola DSM 101718T exhibit a number of genes involved in amino acid metabolism, cell wall biogenesis and replication, recombination and repair mechanism that reduced in both the species.
31796989	11	45	theme	common	966:971	arg1	ancestor					973:980	last universal common ancestor	951:980	last universal common ancestor	951:980	Analysis of genomic reduction events in the evolutionary path from the LUCA (last universal common ancestor) to G. mishrai LMG 29155T and G. halophytocola DSM 101718T exhibit a number of genes involved in amino acid metabolism, cell wall biogenesis and replication, recombination and repair mechanism that reduced in both the species.
31796989	11	45	theme	common	966:971	arg1	LUCA					945:948	the LUCA	941:948	the LUCA (last universal common ancestor)	941:981	Analysis of genomic reduction events in the evolutionary path from the LUCA (last universal common ancestor) to G. mishrai LMG 29155T and G. halophytocola DSM 101718T exhibit a number of genes involved in amino acid metabolism, cell wall biogenesis and replication, recombination and repair mechanism that reduced in both the species.
31796989	8	46	theme	rRNA	593:596	arg1	similarity					598:607	a maximum 16S rRNA similarity	579:607	a maximum 16S rRNA similarity of 99.36% with Glutamicibacter halophytocola DSM 101718T	579:664	Strain S5-52T showed a maximum 16S rRNA similarity of 99.36% with Glutamicibacter halophytocola DSM 101718T.
31796989	12	47	theme	strain	1293:1298	arg1	S5-52T					1300:1305	the strain S5-52T	1289:1305	the strain S5-52T	1289:1305	Based on phenotypic, chemotaxonomic properties and comparative genomic studies, the strain S5-52T is considered a novel species of the genus Glutamicibacter, for which the name Glutamicibacter mishrai sp.
31796989	11	48	theme	genes	1061:1065	arg1	number					1051:1056	a number	1049:1056	a number of genes involved in amino acid metabolism, cell wall biogenesis and replication, recombination and repair mechanism that reduced in both the species	1049:1206	Analysis of genomic reduction events in the evolutionary path from the LUCA (last universal common ancestor) to G. mishrai LMG 29155T and G. halophytocola DSM 101718T exhibit a number of genes involved in amino acid metabolism, cell wall biogenesis and replication, recombination and repair mechanism that reduced in both the species.
31796989	12	49	theme	name	1381:1384	arg1	sp					1410:1411	the name Glutamicibacter mishrai sp	1377:1411	the name Glutamicibacter mishrai sp	1377:1411	Based on phenotypic, chemotaxonomic properties and comparative genomic studies, the strain S5-52T is considered a novel species of the genus Glutamicibacter, for which the name Glutamicibacter mishrai sp.
31796989	8	50	theme	halophytocola	640:652	arg1	101718T					658:664	Glutamicibacter halophytocola DSM 101718T	624:664	Glutamicibacter halophytocola DSM 101718T	624:664	Strain S5-52T showed a maximum 16S rRNA similarity of 99.36% with Glutamicibacter halophytocola DSM 101718T.
31796989	4	51	theme	DNA	311:313	arg1	content					319:325	The DNA G+C content	307:325	The DNA G+C content	307:325	The DNA G+C content was 58.7 mol%.
31796989	4	51	theme	DNA	311:313	arg1	%					339:339	58.7 mol%	331:339	58.7 mol%	331:339	The DNA G+C content was 58.7 mol%.
31796989	1	52	from	Sea	84:86	arg1	veroni					64:69	the coral Favia veroni	48:69	the coral Favia veroni from Andaman Sea	48:86	nov., isolated from the coral Favia veroni from Andaman Sea.
31796989	12	53	theme	comparative	1260:1270	arg1	studies					1280:1286	comparative genomic studies	1260:1286	comparative genomic studies	1260:1286	Based on phenotypic, chemotaxonomic properties and comparative genomic studies, the strain S5-52T is considered a novel species of the genus Glutamicibacter, for which the name Glutamicibacter mishrai sp.
31796989	12	54	theme	mishrai	1402:1408	arg1	sp					1410:1411	the name Glutamicibacter mishrai sp	1377:1411	the name Glutamicibacter mishrai sp	1377:1411	Based on phenotypic, chemotaxonomic properties and comparative genomic studies, the strain S5-52T is considered a novel species of the genus Glutamicibacter, for which the name Glutamicibacter mishrai sp.
31796989	11	55	theme	amino	1079:1083	arg1	metabolism					1090:1099	amino acid metabolism	1079:1099	amino acid metabolism	1079:1099	Analysis of genomic reduction events in the evolutionary path from the LUCA (last universal common ancestor) to G. mishrai LMG 29155T and G. halophytocola DSM 101718T exhibit a number of genes involved in amino acid metabolism, cell wall biogenesis and replication, recombination and repair mechanism that reduced in both the species.
31796989	7	56	theme	peptidoglycan	530:542	arg1	A4α					553:555	A4α	553:555	A4α	553:555	The peptidoglycan type was A4α.
31796989	7	56	theme	peptidoglycan	530:542	arg1	type					544:547	The peptidoglycan type	526:547	The peptidoglycan type	526:547	The peptidoglycan type was A4α.
31796989	11	57	theme	last	951:954	arg1	ancestor					973:980	last universal common ancestor	951:980	last universal common ancestor	951:980	Analysis of genomic reduction events in the evolutionary path from the LUCA (last universal common ancestor) to G. mishrai LMG 29155T and G. halophytocola DSM 101718T exhibit a number of genes involved in amino acid metabolism, cell wall biogenesis and replication, recombination and repair mechanism that reduced in both the species.
31796989	11	57	theme	last	951:954	arg1	LUCA					945:948	the LUCA	941:948	the LUCA (last universal common ancestor)	941:981	Analysis of genomic reduction events in the evolutionary path from the LUCA (last universal common ancestor) to G. mishrai LMG 29155T and G. halophytocola DSM 101718T exhibit a number of genes involved in amino acid metabolism, cell wall biogenesis and replication, recombination and repair mechanism that reduced in both the species.
31796989	11	58	from	Analysis	874:881	arg1	path					931:934	the evolutionary path	914:934	the evolutionary path from the LUCA (last universal common ancestor) to G. mishrai LMG 29155T and G. halophytocola DSM 101718T	914:1039	Analysis of genomic reduction events in the evolutionary path from the LUCA (last universal common ancestor) to G. mishrai LMG 29155T and G. halophytocola DSM 101718T exhibit a number of genes involved in amino acid metabolism, cell wall biogenesis and replication, recombination and repair mechanism that reduced in both the species.
31796989	8	59	theme	Strain	558:563	arg1	S5-52T					565:570	Strain S5-52T	558:570	Strain S5-52T	558:570	Strain S5-52T showed a maximum 16S rRNA similarity of 99.36% with Glutamicibacter halophytocola DSM 101718T.
31796989	11	60	theme	cell	1102:1105	arg1	biogenesis					1112:1121	cell wall biogenesis	1102:1121	cell wall biogenesis	1102:1121	Analysis of genomic reduction events in the evolutionary path from the LUCA (last universal common ancestor) to G. mishrai LMG 29155T and G. halophytocola DSM 101718T exhibit a number of genes involved in amino acid metabolism, cell wall biogenesis and replication, recombination and repair mechanism that reduced in both the species.
31796989	11	61	theme	universal	956:964	arg1	ancestor					973:980	last universal common ancestor	951:980	last universal common ancestor	951:980	Analysis of genomic reduction events in the evolutionary path from the LUCA (last universal common ancestor) to G. mishrai LMG 29155T and G. halophytocola DSM 101718T exhibit a number of genes involved in amino acid metabolism, cell wall biogenesis and replication, recombination and repair mechanism that reduced in both the species.
31796989	11	61	theme	universal	956:964	arg1	LUCA					945:948	the LUCA	941:948	the LUCA (last universal common ancestor)	941:981	Analysis of genomic reduction events in the evolutionary path from the LUCA (last universal common ancestor) to G. mishrai LMG 29155T and G. halophytocola DSM 101718T exhibit a number of genes involved in amino acid metabolism, cell wall biogenesis and replication, recombination and repair mechanism that reduced in both the species.
31796989	2	62	theme	genus	161:165	arg1	Glutamicibacter					167:181	the genus Glutamicibacter	157:181	the genus Glutamicibacter	157:181	A novel aerobic marine actinobacterium (strain S5-52T) belonging to the genus Glutamicibacter was isolated from the coral Favia veroni sampled from the Andaman Sea, India.
31796989	12	63	theme	genomic	1272:1278	arg1	studies					1280:1286	comparative genomic studies	1260:1286	comparative genomic studies	1260:1286	Based on phenotypic, chemotaxonomic properties and comparative genomic studies, the strain S5-52T is considered a novel species of the genus Glutamicibacter, for which the name Glutamicibacter mishrai sp.
31796989	11	64	dep	29155T	1001:1006	arg1	101718T					1033:1039	DSM 101718T	1029:1039	DSM 101718T	1029:1039	Analysis of genomic reduction events in the evolutionary path from the LUCA (last universal common ancestor) to G. mishrai LMG 29155T and G. halophytocola DSM 101718T exhibit a number of genes involved in amino acid metabolism, cell wall biogenesis and replication, recombination and repair mechanism that reduced in both the species.
31796989	2	65	theme	marine	105:110	arg1	actinobacterium					112:126	A novel aerobic marine actinobacterium	89:126	A novel aerobic marine actinobacterium (strain S5-52T) belonging to the genus Glutamicibacter	89:181	A novel aerobic marine actinobacterium (strain S5-52T) belonging to the genus Glutamicibacter was isolated from the coral Favia veroni sampled from the Andaman Sea, India.
31796989	2	65	theme	marine	105:110	arg1	S5-52T					136:141	strain S5-52T	129:141	strain S5-52T	129:141	A novel aerobic marine actinobacterium (strain S5-52T) belonging to the genus Glutamicibacter was isolated from the coral Favia veroni sampled from the Andaman Sea, India.
31796989	11	66	theme	acid	1085:1088	arg1	metabolism					1090:1099	amino acid metabolism	1079:1099	amino acid metabolism	1079:1099	Analysis of genomic reduction events in the evolutionary path from the LUCA (last universal common ancestor) to G. mishrai LMG 29155T and G. halophytocola DSM 101718T exhibit a number of genes involved in amino acid metabolism, cell wall biogenesis and replication, recombination and repair mechanism that reduced in both the species.
31796989	11	67	from	path	931:934	arg1	Analysis					874:881	Analysis	874:881	Analysis of genomic reduction events in the evolutionary path from the LUCA (last universal common ancestor) to G. mishrai LMG 29155T and G. halophytocola DSM 101718T	874:1039	Analysis of genomic reduction events in the evolutionary path from the LUCA (last universal common ancestor) to G. mishrai LMG 29155T and G. halophytocola DSM 101718T exhibit a number of genes involved in amino acid metabolism, cell wall biogenesis and replication, recombination and repair mechanism that reduced in both the species.
31796989	8	68	with	%	617:617	arg1	101718T					658:664	Glutamicibacter halophytocola DSM 101718T	624:664	Glutamicibacter halophytocola DSM 101718T	624:664	Strain S5-52T showed a maximum 16S rRNA similarity of 99.36% with Glutamicibacter halophytocola DSM 101718T.
31796989	5	69	theme	major	346:350	arg1	MK-8					366:369	MK-8	366:369	MK-8	366:369	The major quinones were MK-8 and MK-9.
31796989	5	69	theme	major	346:350	arg1	quinones					352:359	The major quinones	342:359	The major quinones	342:359	The major quinones were MK-8 and MK-9.
31135335	11	0	theme	strain	1534:1539	arg1	BAA-2637T					1555:1563	type strain EPLC-04T=ATCC BAA-2637T	1529:1563	type strain EPLC-04T=ATCC BAA-2637T	1529:1563	nov. is proposed for this novel taxon (type strain EPLC-04T=ATCC BAA-2637T, DSMZ 28595T).
31135335	11	0	theme	strain	1534:1539	arg1	28595T					1571:1576	DSMZ 28595T	1566:1576	DSMZ 28595T	1566:1576	nov. is proposed for this novel taxon (type strain EPLC-04T=ATCC BAA-2637T, DSMZ 28595T).
31135335	3	1	theme	genus	199:203	arg1	Yersinia					205:212	the genus Yersinia	195:212	the genus Yersinia	195:212	A single bacterial isolate, EPLC-04T, was isolated from human feces and identified as representing a member of the genus Yersinia on the basis of phenotypic characteristics, matrix assisted laser desorption ionization time-of-flight mass spectrometry and partial 16S rRNA gene sequencing.
31135335	6	2	theme	%	1179:1179	arg1	value					1166:1170	a bootstrap value	1154:1170	a bootstrap value of 100 %	1154:1179	Phylogenetic reconstructions based on the combination of the four housekeeping genes indicated that the isolate formed a unique branch, supported by a bootstrap value of 100 %.
31135335	5	3	theme	groEL	672:676	arg1	targets					582:588	Multiple genetic targets	565:588	Multiple genetic targets	565:588	Multiple genetic targets, including the complete (1465 bp) 16S rRNA gene sequence and partial sequences of groEL (539 bp), gyrB (935 bp), glnA (525 bp) and recA (535 bp) indicated that the isolate exhibited 98.91, 92.16, 90.81, 92.78 and 89.01 % identity with Yersinia aldovae, 98.98, 91.99, 90.17, 89.77 and 89.55 % identity with Yersinia intermedia, and 99.66, 98.11, 98.50, 98.49 and 98.51 % identity with Y. kristensenii, respectively.
31135335	5	3	theme	groEL	672:676	arg1	sequences					659:667	partial sequences	651:667	partial sequences of groEL (539 bp), gyrB (935 bp), glnA (525 bp) and recA (535 bp)	651:733	Multiple genetic targets, including the complete (1465 bp) 16S rRNA gene sequence and partial sequences of groEL (539 bp), gyrB (935 bp), glnA (525 bp) and recA (535 bp) indicated that the isolate exhibited 98.91, 92.16, 90.81, 92.78 and 89.01 % identity with Yersinia aldovae, 98.98, 91.99, 90.17, 89.77 and 89.55 % identity with Yersinia intermedia, and 99.66, 98.11, 98.50, 98.49 and 98.51 % identity with Y. kristensenii, respectively.
31135335	8	4	theme	phenotypic	1309:1318	arg1	traits					1320:1325	unique phenotypic traits	1302:1325	unique phenotypic traits	1302:1325	However, the unique phenotypic traits and results of phylogenetic analysis indicate that it represents a novel subspecies of Y. kristensenii.
31135335	3	5	theme	mass	317:320	arg1	spectrometry					322:333	matrix assisted laser desorption ionization time-of-flight mass spectrometry	258:333	matrix assisted laser desorption ionization time-of-flight mass spectrometry	258:333	A single bacterial isolate, EPLC-04T, was isolated from human feces and identified as representing a member of the genus Yersinia on the basis of phenotypic characteristics, matrix assisted laser desorption ionization time-of-flight mass spectrometry and partial 16S rRNA gene sequencing.
31135335	3	5	theme	mass	317:320	arg1	characteristics					241:255	phenotypic characteristics	230:255	phenotypic characteristics	230:255	A single bacterial isolate, EPLC-04T, was isolated from human feces and identified as representing a member of the genus Yersinia on the basis of phenotypic characteristics, matrix assisted laser desorption ionization time-of-flight mass spectrometry and partial 16S rRNA gene sequencing.
31135335	4	6	theme	lipase	510:515	arg1	production					517:526	lipase production	510:526	lipase production	510:526	The isolate's phenotypic profile differed from that described for the most closely related species, Yersinia kristensenii, by exhibiting lipase production and lacking pyrazinamidase activity.
31135335	3	7	theme	Yersinia	205:212	arg1	member					185:190	a member	183:190	a member of the genus Yersinia	183:212	A single bacterial isolate, EPLC-04T, was isolated from human feces and identified as representing a member of the genus Yersinia on the basis of phenotypic characteristics, matrix assisted laser desorption ionization time-of-flight mass spectrometry and partial 16S rRNA gene sequencing.
31135335	4	8	theme	phenotypic	387:396	arg1	profile					398:404	The isolate's phenotypic profile	373:404	The isolate's phenotypic profile	373:404	The isolate's phenotypic profile differed from that described for the most closely related species, Yersinia kristensenii, by exhibiting lipase production and lacking pyrazinamidase activity.
31135335	5	9	with	identity	811:818	arg1	Yersinia					825:832	Yersinia aldovae, 98.98, 91.99, 90.17, 89.77 and 89.55 % identity	825:889	Yersinia	825:832	Multiple genetic targets, including the complete (1465 bp) 16S rRNA gene sequence and partial sequences of groEL (539 bp), gyrB (935 bp), glnA (525 bp) and recA (535 bp) indicated that the isolate exhibited 98.91, 92.16, 90.81, 92.78 and 89.01 % identity with Yersinia aldovae, 98.98, 91.99, 90.17, 89.77 and 89.55 % identity with Yersinia intermedia, and 99.66, 98.11, 98.50, 98.49 and 98.51 % identity with Y. kristensenii, respectively.
31135335	3	10	theme	rRNA	351:354	arg1	sequencing					361:370	partial 16S rRNA gene sequencing	339:370	partial 16S rRNA gene sequencing	339:370	A single bacterial isolate, EPLC-04T, was isolated from human feces and identified as representing a member of the genus Yersinia on the basis of phenotypic characteristics, matrix assisted laser desorption ionization time-of-flight mass spectrometry and partial 16S rRNA gene sequencing.
31135335	3	10	theme	rRNA	351:354	arg1	characteristics					241:255	phenotypic characteristics	230:255	phenotypic characteristics	230:255	A single bacterial isolate, EPLC-04T, was isolated from human feces and identified as representing a member of the genus Yersinia on the basis of phenotypic characteristics, matrix assisted laser desorption ionization time-of-flight mass spectrometry and partial 16S rRNA gene sequencing.
31135335	7	11	theme	gene	1220:1223	arg1	sequencing					1225:1234	Digital DNA-DNA homology and 16S rRNA gene sequencing	1182:1234	sequencing	1225:1234	Digital DNA-DNA homology and 16S rRNA gene sequencing identified EPLC-04T as representing Y. kristensenii.
31135335	5	12	theme	1465 bp	615:621	arg1	sequence					638:645	the complete (1465 bp) 16S rRNA gene sequence	601:645	the complete (1465 bp) 16S rRNA gene sequence	601:645	Multiple genetic targets, including the complete (1465 bp) 16S rRNA gene sequence and partial sequences of groEL (539 bp), gyrB (935 bp), glnA (525 bp) and recA (535 bp) indicated that the isolate exhibited 98.91, 92.16, 90.81, 92.78 and 89.01 % identity with Yersinia aldovae, 98.98, 91.99, 90.17, 89.77 and 89.55 % identity with Yersinia intermedia, and 99.66, 98.11, 98.50, 98.49 and 98.51 % identity with Y. kristensenii, respectively.
31135335	7	13	theme	Y.	1272:1273	arg1	kristensenii					1275:1286	Y. kristensenii	1272:1286	Y. kristensenii	1272:1286	Digital DNA-DNA homology and 16S rRNA gene sequencing identified EPLC-04T as representing Y. kristensenii.
31135335	3	14	theme	gene	356:359	arg1	sequencing					361:370	partial 16S rRNA gene sequencing	339:370	partial 16S rRNA gene sequencing	339:370	A single bacterial isolate, EPLC-04T, was isolated from human feces and identified as representing a member of the genus Yersinia on the basis of phenotypic characteristics, matrix assisted laser desorption ionization time-of-flight mass spectrometry and partial 16S rRNA gene sequencing.
31135335	3	14	theme	gene	356:359	arg1	characteristics					241:255	phenotypic characteristics	230:255	phenotypic characteristics	230:255	A single bacterial isolate, EPLC-04T, was isolated from human feces and identified as representing a member of the genus Yersinia on the basis of phenotypic characteristics, matrix assisted laser desorption ionization time-of-flight mass spectrometry and partial 16S rRNA gene sequencing.
31135335	5	15	theme	%	958:958	arg1	identity					960:967	99.66, 98.11, 98.50, 98.49 and 98.51 % identity	921:967	99.66, 98.11, 98.50, 98.49 and 98.51 % identity	921:967	Multiple genetic targets, including the complete (1465 bp) 16S rRNA gene sequence and partial sequences of groEL (539 bp), gyrB (935 bp), glnA (525 bp) and recA (535 bp) indicated that the isolate exhibited 98.91, 92.16, 90.81, 92.78 and 89.01 % identity with Yersinia aldovae, 98.98, 91.99, 90.17, 89.77 and 89.55 % identity with Yersinia intermedia, and 99.66, 98.11, 98.50, 98.49 and 98.51 % identity with Y. kristensenii, respectively.
31135335	3	16	theme	human	140:144	arg1	feces					146:150	human feces	140:150	human feces	140:150	A single bacterial isolate, EPLC-04T, was isolated from human feces and identified as representing a member of the genus Yersinia on the basis of phenotypic characteristics, matrix assisted laser desorption ionization time-of-flight mass spectrometry and partial 16S rRNA gene sequencing.
31135335	3	17	theme	partial	339:345	arg1	sequencing					361:370	partial 16S rRNA gene sequencing	339:370	partial 16S rRNA gene sequencing	339:370	A single bacterial isolate, EPLC-04T, was isolated from human feces and identified as representing a member of the genus Yersinia on the basis of phenotypic characteristics, matrix assisted laser desorption ionization time-of-flight mass spectrometry and partial 16S rRNA gene sequencing.
31135335	3	17	theme	partial	339:345	arg1	characteristics					241:255	phenotypic characteristics	230:255	phenotypic characteristics	230:255	A single bacterial isolate, EPLC-04T, was isolated from human feces and identified as representing a member of the genus Yersinia on the basis of phenotypic characteristics, matrix assisted laser desorption ionization time-of-flight mass spectrometry and partial 16S rRNA gene sequencing.
31135335	8	18	theme	novel	1394:1398	arg1	subspecies					1400:1409	a novel subspecies	1392:1409	a novel subspecies of Y. kristensenii	1392:1428	However, the unique phenotypic traits and results of phylogenetic analysis indicate that it represents a novel subspecies of Y. kristensenii.
31135335	5	19	theme	16S	624:626	arg1	sequence					638:645	the complete (1465 bp) 16S rRNA gene sequence	601:645	the complete (1465 bp) 16S rRNA gene sequence	601:645	Multiple genetic targets, including the complete (1465 bp) 16S rRNA gene sequence and partial sequences of groEL (539 bp), gyrB (935 bp), glnA (525 bp) and recA (535 bp) indicated that the isolate exhibited 98.91, 92.16, 90.81, 92.78 and 89.01 % identity with Yersinia aldovae, 98.98, 91.99, 90.17, 89.77 and 89.55 % identity with Yersinia intermedia, and 99.66, 98.11, 98.50, 98.49 and 98.51 % identity with Y. kristensenii, respectively.
31135335	5	20	dep	Yersinia	825:832	arg1	aldovae					834:840	Yersinia aldovae, 98.98, 91.99, 90.17, 89.77 and 89.55 % identity	825:889	aldovae	834:840	Multiple genetic targets, including the complete (1465 bp) 16S rRNA gene sequence and partial sequences of groEL (539 bp), gyrB (935 bp), glnA (525 bp) and recA (535 bp) indicated that the isolate exhibited 98.91, 92.16, 90.81, 92.78 and 89.01 % identity with Yersinia aldovae, 98.98, 91.99, 90.17, 89.77 and 89.55 % identity with Yersinia intermedia, and 99.66, 98.11, 98.50, 98.49 and 98.51 % identity with Y. kristensenii, respectively.
31135335	5	20	dep	Yersinia	825:832	arg1	identity					882:889	98.98, 91.99, 90.17, 89.77 and 89.55 % identity	843:889	98.98, 91.99, 90.17, 89.77 and 89.55 % identity	843:889	Multiple genetic targets, including the complete (1465 bp) 16S rRNA gene sequence and partial sequences of groEL (539 bp), gyrB (935 bp), glnA (525 bp) and recA (535 bp) indicated that the isolate exhibited 98.91, 92.16, 90.81, 92.78 and 89.01 % identity with Yersinia aldovae, 98.98, 91.99, 90.17, 89.77 and 89.55 % identity with Yersinia intermedia, and 99.66, 98.11, 98.50, 98.49 and 98.51 % identity with Y. kristensenii, respectively.
31135335	5	21	theme	gyrB	688:691	arg1	targets					582:588	Multiple genetic targets	565:588	Multiple genetic targets	565:588	Multiple genetic targets, including the complete (1465 bp) 16S rRNA gene sequence and partial sequences of groEL (539 bp), gyrB (935 bp), glnA (525 bp) and recA (535 bp) indicated that the isolate exhibited 98.91, 92.16, 90.81, 92.78 and 89.01 % identity with Yersinia aldovae, 98.98, 91.99, 90.17, 89.77 and 89.55 % identity with Yersinia intermedia, and 99.66, 98.11, 98.50, 98.49 and 98.51 % identity with Y. kristensenii, respectively.
31135335	5	21	theme	gyrB	688:691	arg1	sequences					659:667	partial sequences	651:667	partial sequences of groEL (539 bp), gyrB (935 bp), glnA (525 bp) and recA (535 bp)	651:733	Multiple genetic targets, including the complete (1465 bp) 16S rRNA gene sequence and partial sequences of groEL (539 bp), gyrB (935 bp), glnA (525 bp) and recA (535 bp) indicated that the isolate exhibited 98.91, 92.16, 90.81, 92.78 and 89.01 % identity with Yersinia aldovae, 98.98, 91.99, 90.17, 89.77 and 89.55 % identity with Yersinia intermedia, and 99.66, 98.11, 98.50, 98.49 and 98.51 % identity with Y. kristensenii, respectively.
31135335	3	22	theme	16S	347:349	arg1	sequencing					361:370	partial 16S rRNA gene sequencing	339:370	partial 16S rRNA gene sequencing	339:370	A single bacterial isolate, EPLC-04T, was isolated from human feces and identified as representing a member of the genus Yersinia on the basis of phenotypic characteristics, matrix assisted laser desorption ionization time-of-flight mass spectrometry and partial 16S rRNA gene sequencing.
31135335	3	22	theme	16S	347:349	arg1	characteristics					241:255	phenotypic characteristics	230:255	phenotypic characteristics	230:255	A single bacterial isolate, EPLC-04T, was isolated from human feces and identified as representing a member of the genus Yersinia on the basis of phenotypic characteristics, matrix assisted laser desorption ionization time-of-flight mass spectrometry and partial 16S rRNA gene sequencing.
31135335	8	23	theme	Y.	1414:1415	arg1	kristensenii					1417:1428	Y. kristensenii	1414:1428	Y. kristensenii	1414:1428	However, the unique phenotypic traits and results of phylogenetic analysis indicate that it represents a novel subspecies of Y. kristensenii.
31135335	5	24	theme	rRNA	628:631	arg1	sequence					638:645	the complete (1465 bp) 16S rRNA gene sequence	601:645	the complete (1465 bp) 16S rRNA gene sequence	601:645	Multiple genetic targets, including the complete (1465 bp) 16S rRNA gene sequence and partial sequences of groEL (539 bp), gyrB (935 bp), glnA (525 bp) and recA (535 bp) indicated that the isolate exhibited 98.91, 92.16, 90.81, 92.78 and 89.01 % identity with Yersinia aldovae, 98.98, 91.99, 90.17, 89.77 and 89.55 % identity with Yersinia intermedia, and 99.66, 98.11, 98.50, 98.49 and 98.51 % identity with Y. kristensenii, respectively.
31135335	9	25	theme	name	1435:1438	arg1	kristenseniisubsp					1449:1465	The name Yersinia kristenseniisubsp	1431:1465	The name Yersinia kristenseniisubsp.	1431:1466	The name Yersinia kristenseniisubsp.
31135335	5	26	theme	glnA	703:706	arg1	targets					582:588	Multiple genetic targets	565:588	Multiple genetic targets	565:588	Multiple genetic targets, including the complete (1465 bp) 16S rRNA gene sequence and partial sequences of groEL (539 bp), gyrB (935 bp), glnA (525 bp) and recA (535 bp) indicated that the isolate exhibited 98.91, 92.16, 90.81, 92.78 and 89.01 % identity with Yersinia aldovae, 98.98, 91.99, 90.17, 89.77 and 89.55 % identity with Yersinia intermedia, and 99.66, 98.11, 98.50, 98.49 and 98.51 % identity with Y. kristensenii, respectively.
31135335	5	26	theme	glnA	703:706	arg1	sequences					659:667	partial sequences	651:667	partial sequences of groEL (539 bp), gyrB (935 bp), glnA (525 bp) and recA (535 bp)	651:733	Multiple genetic targets, including the complete (1465 bp) 16S rRNA gene sequence and partial sequences of groEL (539 bp), gyrB (935 bp), glnA (525 bp) and recA (535 bp) indicated that the isolate exhibited 98.91, 92.16, 90.81, 92.78 and 89.01 % identity with Yersinia aldovae, 98.98, 91.99, 90.17, 89.77 and 89.55 % identity with Yersinia intermedia, and 99.66, 98.11, 98.50, 98.49 and 98.51 % identity with Y. kristensenii, respectively.
31135335	0	27	theme	kristensenii	9:20	arg1	subsp					22:26	Yersinia kristensenii subsp	0:26	Yersinia kristensenii subsp.	0:27	Yersinia kristensenii subsp.
31135335	5	28	theme	recA	721:724	arg1	targets					582:588	Multiple genetic targets	565:588	Multiple genetic targets	565:588	Multiple genetic targets, including the complete (1465 bp) 16S rRNA gene sequence and partial sequences of groEL (539 bp), gyrB (935 bp), glnA (525 bp) and recA (535 bp) indicated that the isolate exhibited 98.91, 92.16, 90.81, 92.78 and 89.01 % identity with Yersinia aldovae, 98.98, 91.99, 90.17, 89.77 and 89.55 % identity with Yersinia intermedia, and 99.66, 98.11, 98.50, 98.49 and 98.51 % identity with Y. kristensenii, respectively.
31135335	5	28	theme	recA	721:724	arg1	sequences					659:667	partial sequences	651:667	partial sequences of groEL (539 bp), gyrB (935 bp), glnA (525 bp) and recA (535 bp)	651:733	Multiple genetic targets, including the complete (1465 bp) 16S rRNA gene sequence and partial sequences of groEL (539 bp), gyrB (935 bp), glnA (525 bp) and recA (535 bp) indicated that the isolate exhibited 98.91, 92.16, 90.81, 92.78 and 89.01 % identity with Yersinia aldovae, 98.98, 91.99, 90.17, 89.77 and 89.55 % identity with Yersinia intermedia, and 99.66, 98.11, 98.50, 98.49 and 98.51 % identity with Y. kristensenii, respectively.
31135335	5	29	theme	gene	633:636	arg1	sequence					638:645	the complete (1465 bp) 16S rRNA gene sequence	601:645	the complete (1465 bp) 16S rRNA gene sequence	601:645	Multiple genetic targets, including the complete (1465 bp) 16S rRNA gene sequence and partial sequences of groEL (539 bp), gyrB (935 bp), glnA (525 bp) and recA (535 bp) indicated that the isolate exhibited 98.91, 92.16, 90.81, 92.78 and 89.01 % identity with Yersinia aldovae, 98.98, 91.99, 90.17, 89.77 and 89.55 % identity with Yersinia intermedia, and 99.66, 98.11, 98.50, 98.49 and 98.51 % identity with Y. kristensenii, respectively.
31135335	11	30	dep	taxon	1522:1526	arg1	BAA-2637T					1555:1563	type strain EPLC-04T=ATCC BAA-2637T	1529:1563	type strain EPLC-04T=ATCC BAA-2637T	1529:1563	nov. is proposed for this novel taxon (type strain EPLC-04T=ATCC BAA-2637T, DSMZ 28595T).
31135335	11	30	dep	taxon	1522:1526	arg1	28595T					1571:1576	DSMZ 28595T	1566:1576	DSMZ 28595T	1566:1576	nov. is proposed for this novel taxon (type strain EPLC-04T=ATCC BAA-2637T, DSMZ 28595T).
31135335	9	31	theme	Yersinia	1440:1447	arg1	kristenseniisubsp					1449:1465	The name Yersinia kristenseniisubsp	1431:1465	The name Yersinia kristenseniisubsp.	1431:1466	The name Yersinia kristenseniisubsp.
31135335	11	32	theme	DSMZ	1566:1569	arg1	BAA-2637T					1555:1563	type strain EPLC-04T=ATCC BAA-2637T	1529:1563	type strain EPLC-04T=ATCC BAA-2637T	1529:1563	nov. is proposed for this novel taxon (type strain EPLC-04T=ATCC BAA-2637T, DSMZ 28595T).
31135335	11	32	theme	DSMZ	1566:1569	arg1	28595T					1571:1576	DSMZ 28595T	1566:1576	DSMZ 28595T	1566:1576	nov. is proposed for this novel taxon (type strain EPLC-04T=ATCC BAA-2637T, DSMZ 28595T).
31135335	0	33	theme	Yersinia	0:7	arg1	subsp					22:26	Yersinia kristensenii subsp	0:26	Yersinia kristensenii subsp.	0:27	Yersinia kristensenii subsp.
31135335	2	34	theme	human	71:75	arg1	feces					77:81	human feces	71:81	human feces	71:81	nov., isolated from human feces.
31135335	5	35	theme	partial	651:657	arg1	sequences					659:667	partial sequences	651:667	partial sequences of groEL (539 bp), gyrB (935 bp), glnA (525 bp) and recA (535 bp)	651:733	Multiple genetic targets, including the complete (1465 bp) 16S rRNA gene sequence and partial sequences of groEL (539 bp), gyrB (935 bp), glnA (525 bp) and recA (535 bp) indicated that the isolate exhibited 98.91, 92.16, 90.81, 92.78 and 89.01 % identity with Yersinia aldovae, 98.98, 91.99, 90.17, 89.77 and 89.55 % identity with Yersinia intermedia, and 99.66, 98.11, 98.50, 98.49 and 98.51 % identity with Y. kristensenii, respectively.
31135335	8	36	theme	kristensenii	1417:1428	arg1	subspecies					1400:1409	a novel subspecies	1392:1409	a novel subspecies of Y. kristensenii	1392:1428	However, the unique phenotypic traits and results of phylogenetic analysis indicate that it represents a novel subspecies of Y. kristensenii.
31135335	7	37	theme	16S	1211:1213	arg1	sequencing					1225:1234	Digital DNA-DNA homology and 16S rRNA gene sequencing	1182:1234	sequencing	1225:1234	Digital DNA-DNA homology and 16S rRNA gene sequencing identified EPLC-04T as representing Y. kristensenii.
31135335	3	38	theme	single	86:91	arg1	EPLC-04T					112:119	EPLC-04T	112:119	EPLC-04T	112:119	A single bacterial isolate, EPLC-04T, was isolated from human feces and identified as representing a member of the genus Yersinia on the basis of phenotypic characteristics, matrix assisted laser desorption ionization time-of-flight mass spectrometry and partial 16S rRNA gene sequencing.
31135335	3	38	theme	single	86:91	arg1	isolate					103:109	A single bacterial isolate	84:109	A single bacterial isolate	84:109	A single bacterial isolate, EPLC-04T, was isolated from human feces and identified as representing a member of the genus Yersinia on the basis of phenotypic characteristics, matrix assisted laser desorption ionization time-of-flight mass spectrometry and partial 16S rRNA gene sequencing.
31135335	3	39	theme	matrix	258:263	arg1	spectrometry					322:333	matrix assisted laser desorption ionization time-of-flight mass spectrometry	258:333	matrix assisted laser desorption ionization time-of-flight mass spectrometry	258:333	A single bacterial isolate, EPLC-04T, was isolated from human feces and identified as representing a member of the genus Yersinia on the basis of phenotypic characteristics, matrix assisted laser desorption ionization time-of-flight mass spectrometry and partial 16S rRNA gene sequencing.
31135335	3	39	theme	matrix	258:263	arg1	characteristics					241:255	phenotypic characteristics	230:255	phenotypic characteristics	230:255	A single bacterial isolate, EPLC-04T, was isolated from human feces and identified as representing a member of the genus Yersinia on the basis of phenotypic characteristics, matrix assisted laser desorption ionization time-of-flight mass spectrometry and partial 16S rRNA gene sequencing.
31135335	6	40	theme	Phylogenetic	1005:1016	arg1	reconstructions					1018:1032	Phylogenetic reconstructions	1005:1032	Phylogenetic reconstructions based on the combination of the four housekeeping genes	1005:1088	Phylogenetic reconstructions based on the combination of the four housekeeping genes indicated that the isolate formed a unique branch, supported by a bootstrap value of 100 %.
31135335	8	41	dep	traits	1320:1325	arg1	the					1298:1300	the	1298:1300	the	1298:1300	However, the unique phenotypic traits and results of phylogenetic analysis indicate that it represents a novel subspecies of Y. kristensenii.
31135335	8	42	theme	unique	1302:1307	arg1	traits					1320:1325	unique phenotypic traits	1302:1325	unique phenotypic traits	1302:1325	However, the unique phenotypic traits and results of phylogenetic analysis indicate that it represents a novel subspecies of Y. kristensenii.
31135335	4	43	dep	species	464:470	arg1	Yersinia					473:480	the most closely related species, Yersinia kristensenii	439:493	Yersinia	473:480	The isolate's phenotypic profile differed from that described for the most closely related species, Yersinia kristensenii, by exhibiting lipase production and lacking pyrazinamidase activity.
31135335	4	43	dep	species	464:470	arg1	kristensenii					482:493	the most closely related species, Yersinia kristensenii	439:493	kristensenii	482:493	The isolate's phenotypic profile differed from that described for the most closely related species, Yersinia kristensenii, by exhibiting lipase production and lacking pyrazinamidase activity.
31135335	3	44	theme	phenotypic	230:239	arg1	spectrometry					322:333	matrix assisted laser desorption ionization time-of-flight mass spectrometry	258:333	matrix assisted laser desorption ionization time-of-flight mass spectrometry	258:333	A single bacterial isolate, EPLC-04T, was isolated from human feces and identified as representing a member of the genus Yersinia on the basis of phenotypic characteristics, matrix assisted laser desorption ionization time-of-flight mass spectrometry and partial 16S rRNA gene sequencing.
31135335	3	44	theme	phenotypic	230:239	arg1	sequencing					361:370	partial 16S rRNA gene sequencing	339:370	partial 16S rRNA gene sequencing	339:370	A single bacterial isolate, EPLC-04T, was isolated from human feces and identified as representing a member of the genus Yersinia on the basis of phenotypic characteristics, matrix assisted laser desorption ionization time-of-flight mass spectrometry and partial 16S rRNA gene sequencing.
31135335	3	44	theme	phenotypic	230:239	arg1	characteristics					241:255	phenotypic characteristics	230:255	phenotypic characteristics	230:255	A single bacterial isolate, EPLC-04T, was isolated from human feces and identified as representing a member of the genus Yersinia on the basis of phenotypic characteristics, matrix assisted laser desorption ionization time-of-flight mass spectrometry and partial 16S rRNA gene sequencing.
31135335	5	45	theme	Multiple	565:572	arg1	targets					582:588	Multiple genetic targets	565:588	Multiple genetic targets	565:588	Multiple genetic targets, including the complete (1465 bp) 16S rRNA gene sequence and partial sequences of groEL (539 bp), gyrB (935 bp), glnA (525 bp) and recA (535 bp) indicated that the isolate exhibited 98.91, 92.16, 90.81, 92.78 and 89.01 % identity with Yersinia aldovae, 98.98, 91.99, 90.17, 89.77 and 89.55 % identity with Yersinia intermedia, and 99.66, 98.11, 98.50, 98.49 and 98.51 % identity with Y. kristensenii, respectively.
31135335	6	46	theme	bootstrap	1156:1164	arg1	value					1166:1170	a bootstrap value	1154:1170	a bootstrap value of 100 %	1154:1179	Phylogenetic reconstructions based on the combination of the four housekeeping genes indicated that the isolate formed a unique branch, supported by a bootstrap value of 100 %.
31135335	7	47	theme	Digital	1182:1188	arg1	homology					1198:1205	Digital DNA-DNA homology and 16S rRNA gene sequencing	1182:1234	homology	1198:1205	Digital DNA-DNA homology and 16S rRNA gene sequencing identified EPLC-04T as representing Y. kristensenii.
31135335	7	48	theme	DNA-DNA	1190:1196	arg1	homology					1198:1205	Digital DNA-DNA homology and 16S rRNA gene sequencing	1182:1234	homology	1198:1205	Digital DNA-DNA homology and 16S rRNA gene sequencing identified EPLC-04T as representing Y. kristensenii.
31135335	3	49	theme	characteristics	241:255	arg1	basis					221:225	the basis	217:225	the basis of phenotypic characteristics, matrix assisted laser desorption ionization time-of-flight mass spectrometry and partial 16S rRNA gene sequencing	217:370	A single bacterial isolate, EPLC-04T, was isolated from human feces and identified as representing a member of the genus Yersinia on the basis of phenotypic characteristics, matrix assisted laser desorption ionization time-of-flight mass spectrometry and partial 16S rRNA gene sequencing.
31135335	4	50	theme	related	456:462	arg1	species					464:470	the most closely related species	439:470	the most closely related species	439:470	The isolate's phenotypic profile differed from that described for the most closely related species, Yersinia kristensenii, by exhibiting lipase production and lacking pyrazinamidase activity.
31135335	5	51	dep	Y.	974:975	arg1	kristensenii					977:988	Y. kristensenii	974:988	Y. kristensenii	974:988	Multiple genetic targets, including the complete (1465 bp) 16S rRNA gene sequence and partial sequences of groEL (539 bp), gyrB (935 bp), glnA (525 bp) and recA (535 bp) indicated that the isolate exhibited 98.91, 92.16, 90.81, 92.78 and 89.01 % identity with Yersinia aldovae, 98.98, 91.99, 90.17, 89.77 and 89.55 % identity with Yersinia intermedia, and 99.66, 98.11, 98.50, 98.49 and 98.51 % identity with Y. kristensenii, respectively.
31135335	3	52	theme	desorption	280:289	arg1	spectrometry					322:333	matrix assisted laser desorption ionization time-of-flight mass spectrometry	258:333	matrix assisted laser desorption ionization time-of-flight mass spectrometry	258:333	A single bacterial isolate, EPLC-04T, was isolated from human feces and identified as representing a member of the genus Yersinia on the basis of phenotypic characteristics, matrix assisted laser desorption ionization time-of-flight mass spectrometry and partial 16S rRNA gene sequencing.
31135335	3	52	theme	desorption	280:289	arg1	characteristics					241:255	phenotypic characteristics	230:255	phenotypic characteristics	230:255	A single bacterial isolate, EPLC-04T, was isolated from human feces and identified as representing a member of the genus Yersinia on the basis of phenotypic characteristics, matrix assisted laser desorption ionization time-of-flight mass spectrometry and partial 16S rRNA gene sequencing.
31135335	11	53	theme	EPLC-04T=ATCC	1541:1553	arg1	BAA-2637T					1555:1563	type strain EPLC-04T=ATCC BAA-2637T	1529:1563	type strain EPLC-04T=ATCC BAA-2637T	1529:1563	nov. is proposed for this novel taxon (type strain EPLC-04T=ATCC BAA-2637T, DSMZ 28595T).
31135335	11	53	theme	EPLC-04T=ATCC	1541:1553	arg1	28595T					1571:1576	DSMZ 28595T	1566:1576	DSMZ 28595T	1566:1576	nov. is proposed for this novel taxon (type strain EPLC-04T=ATCC BAA-2637T, DSMZ 28595T).
31135335	4	54	theme	pyrazinamidase	540:553	arg1	activity					555:562	pyrazinamidase activity	540:562	pyrazinamidase activity	540:562	The isolate's phenotypic profile differed from that described for the most closely related species, Yersinia kristensenii, by exhibiting lipase production and lacking pyrazinamidase activity.
31135335	3	55	theme	ionization	291:300	arg1	spectrometry					322:333	matrix assisted laser desorption ionization time-of-flight mass spectrometry	258:333	matrix assisted laser desorption ionization time-of-flight mass spectrometry	258:333	A single bacterial isolate, EPLC-04T, was isolated from human feces and identified as representing a member of the genus Yersinia on the basis of phenotypic characteristics, matrix assisted laser desorption ionization time-of-flight mass spectrometry and partial 16S rRNA gene sequencing.
31135335	3	55	theme	ionization	291:300	arg1	characteristics					241:255	phenotypic characteristics	230:255	phenotypic characteristics	230:255	A single bacterial isolate, EPLC-04T, was isolated from human feces and identified as representing a member of the genus Yersinia on the basis of phenotypic characteristics, matrix assisted laser desorption ionization time-of-flight mass spectrometry and partial 16S rRNA gene sequencing.
31135335	8	56	theme	phylogenetic	1342:1353	arg1	analysis					1355:1362	phylogenetic analysis	1342:1362	phylogenetic analysis	1342:1362	However, the unique phenotypic traits and results of phylogenetic analysis indicate that it represents a novel subspecies of Y. kristensenii.
31135335	3	57	theme	bacterial	93:101	arg1	EPLC-04T					112:119	EPLC-04T	112:119	EPLC-04T	112:119	A single bacterial isolate, EPLC-04T, was isolated from human feces and identified as representing a member of the genus Yersinia on the basis of phenotypic characteristics, matrix assisted laser desorption ionization time-of-flight mass spectrometry and partial 16S rRNA gene sequencing.
31135335	3	57	theme	bacterial	93:101	arg1	isolate					103:109	A single bacterial isolate	84:109	A single bacterial isolate	84:109	A single bacterial isolate, EPLC-04T, was isolated from human feces and identified as representing a member of the genus Yersinia on the basis of phenotypic characteristics, matrix assisted laser desorption ionization time-of-flight mass spectrometry and partial 16S rRNA gene sequencing.
31135335	3	58	attach	isolated	126:133	arg2	EPLC-04T					112:119	EPLC-04T	112:119	EPLC-04T	112:119	A single bacterial isolate, EPLC-04T, was isolated from human feces and identified as representing a member of the genus Yersinia on the basis of phenotypic characteristics, matrix assisted laser desorption ionization time-of-flight mass spectrometry and partial 16S rRNA gene sequencing.
31135335	3	58	attach	isolated	126:133	arg1	feces					146:150	human feces	140:150	human feces	140:150	A single bacterial isolate, EPLC-04T, was isolated from human feces and identified as representing a member of the genus Yersinia on the basis of phenotypic characteristics, matrix assisted laser desorption ionization time-of-flight mass spectrometry and partial 16S rRNA gene sequencing.
31135335	3	58	attach	isolated	126:133	arg2	isolate					103:109	A single bacterial isolate	84:109	A single bacterial isolate	84:109	A single bacterial isolate, EPLC-04T, was isolated from human feces and identified as representing a member of the genus Yersinia on the basis of phenotypic characteristics, matrix assisted laser desorption ionization time-of-flight mass spectrometry and partial 16S rRNA gene sequencing.
31135335	5	59	with	intermedia	905:914	arg1	Y.					974:975	Y.	974:975	Y.	974:975	Multiple genetic targets, including the complete (1465 bp) 16S rRNA gene sequence and partial sequences of groEL (539 bp), gyrB (935 bp), glnA (525 bp) and recA (535 bp) indicated that the isolate exhibited 98.91, 92.16, 90.81, 92.78 and 89.01 % identity with Yersinia aldovae, 98.98, 91.99, 90.17, 89.77 and 89.55 % identity with Yersinia intermedia, and 99.66, 98.11, 98.50, 98.49 and 98.51 % identity with Y. kristensenii, respectively.
31135335	3	60	theme	assisted	265:272	arg1	spectrometry					322:333	matrix assisted laser desorption ionization time-of-flight mass spectrometry	258:333	matrix assisted laser desorption ionization time-of-flight mass spectrometry	258:333	A single bacterial isolate, EPLC-04T, was isolated from human feces and identified as representing a member of the genus Yersinia on the basis of phenotypic characteristics, matrix assisted laser desorption ionization time-of-flight mass spectrometry and partial 16S rRNA gene sequencing.
31135335	3	60	theme	assisted	265:272	arg1	characteristics					241:255	phenotypic characteristics	230:255	phenotypic characteristics	230:255	A single bacterial isolate, EPLC-04T, was isolated from human feces and identified as representing a member of the genus Yersinia on the basis of phenotypic characteristics, matrix assisted laser desorption ionization time-of-flight mass spectrometry and partial 16S rRNA gene sequencing.
31135335	7	61	theme	rRNA	1215:1218	arg1	sequencing					1225:1234	Digital DNA-DNA homology and 16S rRNA gene sequencing	1182:1234	sequencing	1225:1234	Digital DNA-DNA homology and 16S rRNA gene sequencing identified EPLC-04T as representing Y. kristensenii.
31135335	5	62	theme	%	880:880	arg1	identity					882:889	98.98, 91.99, 90.17, 89.77 and 89.55 % identity	843:889	98.98, 91.99, 90.17, 89.77 and 89.55 % identity	843:889	Multiple genetic targets, including the complete (1465 bp) 16S rRNA gene sequence and partial sequences of groEL (539 bp), gyrB (935 bp), glnA (525 bp) and recA (535 bp) indicated that the isolate exhibited 98.91, 92.16, 90.81, 92.78 and 89.01 % identity with Yersinia aldovae, 98.98, 91.99, 90.17, 89.77 and 89.55 % identity with Yersinia intermedia, and 99.66, 98.11, 98.50, 98.49 and 98.51 % identity with Y. kristensenii, respectively.
31135335	5	63	theme	genetic	574:580	arg1	targets					582:588	Multiple genetic targets	565:588	Multiple genetic targets	565:588	Multiple genetic targets, including the complete (1465 bp) 16S rRNA gene sequence and partial sequences of groEL (539 bp), gyrB (935 bp), glnA (525 bp) and recA (535 bp) indicated that the isolate exhibited 98.91, 92.16, 90.81, 92.78 and 89.01 % identity with Yersinia aldovae, 98.98, 91.99, 90.17, 89.77 and 89.55 % identity with Yersinia intermedia, and 99.66, 98.11, 98.50, 98.49 and 98.51 % identity with Y. kristensenii, respectively.
31135335	6	64	theme	unique	1126:1131	arg1	branch					1133:1138	a unique branch	1124:1138	a unique branch	1124:1138	Phylogenetic reconstructions based on the combination of the four housekeeping genes indicated that the isolate formed a unique branch, supported by a bootstrap value of 100 %.
31135335	3	65	theme	laser	274:278	arg1	spectrometry					322:333	matrix assisted laser desorption ionization time-of-flight mass spectrometry	258:333	matrix assisted laser desorption ionization time-of-flight mass spectrometry	258:333	A single bacterial isolate, EPLC-04T, was isolated from human feces and identified as representing a member of the genus Yersinia on the basis of phenotypic characteristics, matrix assisted laser desorption ionization time-of-flight mass spectrometry and partial 16S rRNA gene sequencing.
31135335	3	65	theme	laser	274:278	arg1	characteristics					241:255	phenotypic characteristics	230:255	phenotypic characteristics	230:255	A single bacterial isolate, EPLC-04T, was isolated from human feces and identified as representing a member of the genus Yersinia on the basis of phenotypic characteristics, matrix assisted laser desorption ionization time-of-flight mass spectrometry and partial 16S rRNA gene sequencing.
31135335	6	66	theme	genes	1084:1088	arg1	combination					1047:1057	the combination	1043:1057	the combination of the four housekeeping genes	1043:1088	Phylogenetic reconstructions based on the combination of the four housekeeping genes indicated that the isolate formed a unique branch, supported by a bootstrap value of 100 %.
31135335	8	67	theme	analysis	1355:1362	arg1	results					1331:1337	results	1331:1337	results	1331:1337	However, the unique phenotypic traits and results of phylogenetic analysis indicate that it represents a novel subspecies of Y. kristensenii.
31135335	8	67	theme	analysis	1355:1362	arg1	traits					1320:1325	unique phenotypic traits	1302:1325	unique phenotypic traits	1302:1325	However, the unique phenotypic traits and results of phylogenetic analysis indicate that it represents a novel subspecies of Y. kristensenii.
31135335	6	68	theme	housekeeping	1071:1082	arg1	genes					1084:1088	the four housekeeping genes	1062:1088	the four housekeeping genes	1062:1088	Phylogenetic reconstructions based on the combination of the four housekeeping genes indicated that the isolate formed a unique branch, supported by a bootstrap value of 100 %.
31135335	11	69	theme	novel	1516:1520	arg1	taxon					1522:1526	this novel taxon	1511:1526	this novel taxon (type strain EPLC-04T=ATCC BAA-2637T, DSMZ 28595T)	1511:1577	nov. is proposed for this novel taxon (type strain EPLC-04T=ATCC BAA-2637T, DSMZ 28595T).
31135335	5	70	with	identity	960:967	arg1	Y.					974:975	Y.	974:975	Y.	974:975	Multiple genetic targets, including the complete (1465 bp) 16S rRNA gene sequence and partial sequences of groEL (539 bp), gyrB (935 bp), glnA (525 bp) and recA (535 bp) indicated that the isolate exhibited 98.91, 92.16, 90.81, 92.78 and 89.01 % identity with Yersinia aldovae, 98.98, 91.99, 90.17, 89.77 and 89.55 % identity with Yersinia intermedia, and 99.66, 98.11, 98.50, 98.49 and 98.51 % identity with Y. kristensenii, respectively.
31135335	5	71	theme	complete	605:612	arg1	sequence					638:645	the complete (1465 bp) 16S rRNA gene sequence	601:645	the complete (1465 bp) 16S rRNA gene sequence	601:645	Multiple genetic targets, including the complete (1465 bp) 16S rRNA gene sequence and partial sequences of groEL (539 bp), gyrB (935 bp), glnA (525 bp) and recA (535 bp) indicated that the isolate exhibited 98.91, 92.16, 90.81, 92.78 and 89.01 % identity with Yersinia aldovae, 98.98, 91.99, 90.17, 89.77 and 89.55 % identity with Yersinia intermedia, and 99.66, 98.11, 98.50, 98.49 and 98.51 % identity with Y. kristensenii, respectively.
31135335	11	72	theme	type	1529:1532	arg1	BAA-2637T					1555:1563	type strain EPLC-04T=ATCC BAA-2637T	1529:1563	type strain EPLC-04T=ATCC BAA-2637T	1529:1563	nov. is proposed for this novel taxon (type strain EPLC-04T=ATCC BAA-2637T, DSMZ 28595T).
31135335	11	72	theme	type	1529:1532	arg1	28595T					1571:1576	DSMZ 28595T	1566:1576	DSMZ 28595T	1566:1576	nov. is proposed for this novel taxon (type strain EPLC-04T=ATCC BAA-2637T, DSMZ 28595T).
31135335	5	73	theme	Yersinia	896:903	arg1	intermedia					905:914	Yersinia intermedia, and 99.66, 98.11, 98.50, 98.49 and 98.51 % identity	896:967	intermedia	905:914	Multiple genetic targets, including the complete (1465 bp) 16S rRNA gene sequence and partial sequences of groEL (539 bp), gyrB (935 bp), glnA (525 bp) and recA (535 bp) indicated that the isolate exhibited 98.91, 92.16, 90.81, 92.78 and 89.01 % identity with Yersinia aldovae, 98.98, 91.99, 90.17, 89.77 and 89.55 % identity with Yersinia intermedia, and 99.66, 98.11, 98.50, 98.49 and 98.51 % identity with Y. kristensenii, respectively.
31135335	5	74	theme	%	809:809	arg1	identity					811:818	98.91, 92.16, 90.81, 92.78 and 89.01 % identity	772:818	98.91, 92.16, 90.81, 92.78 and 89.01 % identity with Yersinia aldovae, 98.98, 91.99, 90.17, 89.77 and 89.55 % identity	772:889	Multiple genetic targets, including the complete (1465 bp) 16S rRNA gene sequence and partial sequences of groEL (539 bp), gyrB (935 bp), glnA (525 bp) and recA (535 bp) indicated that the isolate exhibited 98.91, 92.16, 90.81, 92.78 and 89.01 % identity with Yersinia aldovae, 98.98, 91.99, 90.17, 89.77 and 89.55 % identity with Yersinia intermedia, and 99.66, 98.11, 98.50, 98.49 and 98.51 % identity with Y. kristensenii, respectively.
30013041	2	0	theme	bacteria	461:468	arg1	collection					440:449	a collection	438:449	a collection of marine bacteria cultured from different stages of succession on chitin microparticles	438:538	Here, we find that the capacity of communities to degrade particles is highly dependent on community composition using a collection of marine bacteria cultured from different stages of succession on chitin microparticles.
30013041	1	1	from	process	176:182	arg1	cycle					205:209	the global carbon cycle	187:209	the global carbon cycle	187:209	The degradation of particulate organic matter in the ocean is a central process in the global carbon cycle, the mode and tempo of which is determined by the bacterial communities that assemble on particle surfaces.
30013041	5	2	theme	carbon	1039:1044	arg1	turnover					1046:1053	carbon turnover	1039:1053	carbon turnover	1039:1053	Our results suggest that the microscale community ecology of bacteria on particle surfaces can impact the rates of carbon turnover in the ocean.
30013041	0	3	theme	microbial	81:89	arg1	communities					91:101	model marine microbial communities	68:101	model marine microbial communities	68:101	Microscale ecology regulates particulate organic matter turnover in model marine microbial communities.
30013041	5	4	theme	turnover	1046:1053	arg1	rates					1030:1034	the rates	1026:1034	the rates of carbon turnover in the ocean	1026:1066	Our results suggest that the microscale community ecology of bacteria on particle surfaces can impact the rates of carbon turnover in the ocean.
30013041	2	5	theme	succession	504:513	arg1	stages					494:499	different stages	484:499	different stages of succession	484:513	Here, we find that the capacity of communities to degrade particles is highly dependent on community composition using a collection of marine bacteria cultured from different stages of succession on chitin microparticles.
30013041	5	6	theme	microscale	953:962	arg1	ecology					974:980	the microscale community ecology	949:980	the microscale community ecology of bacteria on particle surfaces	949:1013	Our results suggest that the microscale community ecology of bacteria on particle surfaces can impact the rates of carbon turnover in the ocean.
30013041	5	7	theme	community	964:972	arg1	ecology					974:980	the microscale community ecology	949:980	the microscale community ecology of bacteria on particle surfaces	949:1013	Our results suggest that the microscale community ecology of bacteria on particle surfaces can impact the rates of carbon turnover in the ocean.
30013041	3	8	from	particles	677:685	arg1	layer					708:712	the ocean's mixed layer	690:712	the ocean's mixed layer	690:712	Different particle degrading taxa display characteristic particle half-lives that differ by ~170 h, comparable to the residence time of particles in the ocean's mixed layer.
30013041	0	9	from	turnover	56:63	arg1	communities					91:101	model marine microbial communities	68:101	model marine microbial communities	68:101	Microscale ecology regulates particulate organic matter turnover in model marine microbial communities.
30013041	1	10	theme	central	168:174	arg1	degradation					108:118	The degradation	104:118	The degradation of particulate organic matter in the ocean	104:161	The degradation of particulate organic matter in the ocean is a central process in the global carbon cycle, the mode and tempo of which is determined by the bacterial communities that assemble on particle surfaces.
30013041	1	10	theme	central	168:174	arg1	process					176:182	a central process	166:182	a central process in the global carbon cycle, the mode and tempo of which is determined by the bacterial communities that assemble on particle surfaces	166:316	The degradation of particulate organic matter in the ocean is a central process in the global carbon cycle, the mode and tempo of which is determined by the bacterial communities that assemble on particle surfaces.
30013041	2	11	theme	different	484:492	arg1	stages					494:499	different stages	484:499	different stages of succession	484:513	Here, we find that the capacity of communities to degrade particles is highly dependent on community composition using a collection of marine bacteria cultured from different stages of succession on chitin microparticles.
30013041	3	12	theme	mixed	702:706	arg1	layer					708:712	the ocean's mixed layer	690:712	the ocean's mixed layer	690:712	Different particle degrading taxa display characteristic particle half-lives that differ by ~170 h, comparable to the residence time of particles in the ocean's mixed layer.
30013041	0	13	theme	Microscale	0:9	arg1	ecology					11:17	Microscale ecology	0:17	Microscale ecology	0:17	Microscale ecology regulates particulate organic matter turnover in model marine microbial communities.
30013041	2	14	theme	communities	354:364	arg1	capacity					342:349	the capacity	338:349	the capacity of communities to degrade particles	338:385	Here, we find that the capacity of communities to degrade particles is highly dependent on community composition using a collection of marine bacteria cultured from different stages of succession on chitin microparticles.
30013041	2	14	theme	communities	354:364	arg1	dependent					397:405	dependent	397:405	dependent	397:405	Here, we find that the capacity of communities to degrade particles is highly dependent on community composition using a collection of marine bacteria cultured from different stages of succession on chitin microparticles.
30013041	2	15	theme	marine	454:459	arg1	bacteria					461:468	marine bacteria	454:468	marine bacteria cultured from different stages of succession on chitin microparticles	454:538	Here, we find that the capacity of communities to degrade particles is highly dependent on community composition using a collection of marine bacteria cultured from different stages of succession on chitin microparticles.
30013041	4	16	theme	degraders	852:860	arg1	ability					841:847	the ability	837:847	the ability of degraders to colonize and consume particles in a dose dependent manner	837:921	Particle half-lives are in general longer in multispecies communities, where the growth of obligate cross-feeders hinders the ability of degraders to colonize and consume particles in a dose dependent manner.
30013041	3	17	theme	degrading	560:568	arg1	taxa					570:573	Different particle degrading taxa	541:573	Different particle degrading taxa	541:573	Different particle degrading taxa display characteristic particle half-lives that differ by ~170 h, comparable to the residence time of particles in the ocean's mixed layer.
30013041	2	18	theme	community	410:418	arg1	composition					420:430	community composition	410:430	community composition using a collection of marine bacteria cultured from different stages of succession on chitin microparticles	410:538	Here, we find that the capacity of communities to degrade particles is highly dependent on community composition using a collection of marine bacteria cultured from different stages of succession on chitin microparticles.
30013041	0	19	theme	particulate	29:39	arg1	turnover					56:63	particulate organic matter turnover	29:63	particulate organic matter turnover in model marine microbial communities	29:101	Microscale ecology regulates particulate organic matter turnover in model marine microbial communities.
30013041	4	20	theme	cross-feeders	815:827	arg1	growth					796:801	the growth	792:801	the growth of obligate cross-feeders	792:827	Particle half-lives are in general longer in multispecies communities, where the growth of obligate cross-feeders hinders the ability of degraders to colonize and consume particles in a dose dependent manner.
30013041	3	21	theme	Different	541:549	arg1	taxa					570:573	Different particle degrading taxa	541:573	Different particle degrading taxa	541:573	Different particle degrading taxa display characteristic particle half-lives that differ by ~170 h, comparable to the residence time of particles in the ocean's mixed layer.
30013041	4	22	theme	obligate	806:813	arg1	cross-feeders					815:827	obligate cross-feeders	806:827	obligate cross-feeders	806:827	Particle half-lives are in general longer in multispecies communities, where the growth of obligate cross-feeders hinders the ability of degraders to colonize and consume particles in a dose dependent manner.
30013041	1	23	theme	global	191:196	arg1	cycle					205:209	the global carbon cycle	187:209	the global carbon cycle	187:209	The degradation of particulate organic matter in the ocean is a central process in the global carbon cycle, the mode and tempo of which is determined by the bacterial communities that assemble on particle surfaces.
30013041	1	24	theme	bacterial	261:269	arg1	communities					271:281	the bacterial communities	257:281	the bacterial communities that assemble on particle surfaces	257:316	The degradation of particulate organic matter in the ocean is a central process in the global carbon cycle, the mode and tempo of which is determined by the bacterial communities that assemble on particle surfaces.
30013041	4	25	theme	dependent	906:914	arg1	manner					916:921	a dose dependent manner	899:921	a dose dependent manner	899:921	Particle half-lives are in general longer in multispecies communities, where the growth of obligate cross-feeders hinders the ability of degraders to colonize and consume particles in a dose dependent manner.
30013041	3	26	theme	particle	551:558	arg1	taxa					570:573	Different particle degrading taxa	541:573	Different particle degrading taxa	541:573	Different particle degrading taxa display characteristic particle half-lives that differ by ~170 h, comparable to the residence time of particles in the ocean's mixed layer.
30013041	1	27	theme	carbon	198:203	arg1	cycle					205:209	the global carbon cycle	187:209	the global carbon cycle	187:209	The degradation of particulate organic matter in the ocean is a central process in the global carbon cycle, the mode and tempo of which is determined by the bacterial communities that assemble on particle surfaces.
30013041	0	28	theme	matter	49:54	arg1	turnover					56:63	particulate organic matter turnover	29:63	particulate organic matter turnover in model marine microbial communities	29:101	Microscale ecology regulates particulate organic matter turnover in model marine microbial communities.
30013041	2	29	theme	chitin	518:523	arg1	microparticles					525:538	chitin microparticles	518:538	chitin microparticles	518:538	Here, we find that the capacity of communities to degrade particles is highly dependent on community composition using a collection of marine bacteria cultured from different stages of succession on chitin microparticles.
30013041	3	30	theme	particle	598:605	arg1	half-lives					607:616	characteristic particle half-lives	583:616	characteristic particle half-lives that differ by ~170 h, comparable to the residence time of particles in the ocean's mixed layer	583:712	Different particle degrading taxa display characteristic particle half-lives that differ by ~170 h, comparable to the residence time of particles in the ocean's mixed layer.
30013041	1	31	theme	cycle	205:209	arg1	tempo					225:229	tempo	225:229	tempo	225:229	The degradation of particulate organic matter in the ocean is a central process in the global carbon cycle, the mode and tempo of which is determined by the bacterial communities that assemble on particle surfaces.
30013041	1	31	theme	cycle	205:209	arg1	mode					216:219	the mode	212:219	the mode	212:219	The degradation of particulate organic matter in the ocean is a central process in the global carbon cycle, the mode and tempo of which is determined by the bacterial communities that assemble on particle surfaces.
30013041	0	32	theme	organic	41:47	arg1	turnover					56:63	particulate organic matter turnover	29:63	particulate organic matter turnover in model marine microbial communities	29:101	Microscale ecology regulates particulate organic matter turnover in model marine microbial communities.
30013041	3	33	from	time	669:672	arg1	layer					708:712	the ocean's mixed layer	690:712	the ocean's mixed layer	690:712	Different particle degrading taxa display characteristic particle half-lives that differ by ~170 h, comparable to the residence time of particles in the ocean's mixed layer.
30013041	1	34	theme	particulate	123:133	arg1	matter					143:148	particulate organic matter	123:148	particulate organic matter	123:148	The degradation of particulate organic matter in the ocean is a central process in the global carbon cycle, the mode and tempo of which is determined by the bacterial communities that assemble on particle surfaces.
30013041	3	35	theme	characteristic	583:596	arg1	half-lives					607:616	characteristic particle half-lives	583:616	characteristic particle half-lives that differ by ~170 h, comparable to the residence time of particles in the ocean's mixed layer	583:712	Different particle degrading taxa display characteristic particle half-lives that differ by ~170 h, comparable to the residence time of particles in the ocean's mixed layer.
30013041	1	36	theme	organic	135:141	arg1	matter					143:148	particulate organic matter	123:148	particulate organic matter	123:148	The degradation of particulate organic matter in the ocean is a central process in the global carbon cycle, the mode and tempo of which is determined by the bacterial communities that assemble on particle surfaces.
30013041	1	37	from	degradation	108:118	arg1	ocean					157:161	the ocean	153:161	the ocean	153:161	The degradation of particulate organic matter in the ocean is a central process in the global carbon cycle, the mode and tempo of which is determined by the bacterial communities that assemble on particle surfaces.
30013041	1	38	theme	particle	300:307	arg1	surfaces					309:316	particle surfaces	300:316	particle surfaces	300:316	The degradation of particulate organic matter in the ocean is a central process in the global carbon cycle, the mode and tempo of which is determined by the bacterial communities that assemble on particle surfaces.
30013041	3	39	theme	particles	677:685	arg1	time					669:672	the residence time	655:672	the residence time of particles in the ocean's mixed layer	655:712	Different particle degrading taxa display characteristic particle half-lives that differ by ~170 h, comparable to the residence time of particles in the ocean's mixed layer.
30013041	5	40	theme	bacteria	985:992	arg1	ecology					974:980	the microscale community ecology	949:980	the microscale community ecology of bacteria on particle surfaces	949:1013	Our results suggest that the microscale community ecology of bacteria on particle surfaces can impact the rates of carbon turnover in the ocean.
30013041	1	41	theme	matter	143:148	arg1	degradation					108:118	The degradation	104:118	The degradation of particulate organic matter in the ocean	104:161	The degradation of particulate organic matter in the ocean is a central process in the global carbon cycle, the mode and tempo of which is determined by the bacterial communities that assemble on particle surfaces.
30013041	1	41	theme	matter	143:148	arg1	process					176:182	a central process	166:182	a central process in the global carbon cycle, the mode and tempo of which is determined by the bacterial communities that assemble on particle surfaces	166:316	The degradation of particulate organic matter in the ocean is a central process in the global carbon cycle, the mode and tempo of which is determined by the bacterial communities that assemble on particle surfaces.
30013041	4	42	theme	multispecies	760:771	arg1	communities					773:783	multispecies communities	760:783	multispecies communities	760:783	Particle half-lives are in general longer in multispecies communities, where the growth of obligate cross-feeders hinders the ability of degraders to colonize and consume particles in a dose dependent manner.
30013041	3	43	theme	residence	659:667	arg1	time					669:672	the residence time	655:672	the residence time of particles in the ocean's mixed layer	655:712	Different particle degrading taxa display characteristic particle half-lives that differ by ~170 h, comparable to the residence time of particles in the ocean's mixed layer.
30013041	5	44	from	rates	1030:1034	arg1	ocean					1062:1066	the ocean	1058:1066	the ocean	1058:1066	Our results suggest that the microscale community ecology of bacteria on particle surfaces can impact the rates of carbon turnover in the ocean.
30013041	3	45	from	layer	708:712	arg1	time					669:672	the residence time	655:672	the residence time of particles in the ocean's mixed layer	655:712	Different particle degrading taxa display characteristic particle half-lives that differ by ~170 h, comparable to the residence time of particles in the ocean's mixed layer.
30013041	4	46	theme	Particle	715:722	arg1	half-lives					724:733	Particle half-lives	715:733	Particle half-lives	715:733	Particle half-lives are in general longer in multispecies communities, where the growth of obligate cross-feeders hinders the ability of degraders to colonize and consume particles in a dose dependent manner.
30013041	0	47	theme	marine	74:79	arg1	communities					91:101	model marine microbial communities	68:101	model marine microbial communities	68:101	Microscale ecology regulates particulate organic matter turnover in model marine microbial communities.
30013041	5	48	theme	particle	997:1004	arg1	surfaces					1006:1013	particle surfaces	997:1013	particle surfaces	997:1013	Our results suggest that the microscale community ecology of bacteria on particle surfaces can impact the rates of carbon turnover in the ocean.
30013041	0	49	theme	model	68:72	arg1	communities					91:101	model marine microbial communities	68:101	model marine microbial communities	68:101	Microscale ecology regulates particulate organic matter turnover in model marine microbial communities.
30013041	5	50	from	ecology	974:980	arg1	surfaces					1006:1013	particle surfaces	997:1013	particle surfaces	997:1013	Our results suggest that the microscale community ecology of bacteria on particle surfaces can impact the rates of carbon turnover in the ocean.
30320546	5	0	theme	lacti	654:658	arg1	subsp					660:664	L. lacti subsp	651:664	L. lacti subsp	651:664	Furthermore, genomic comparisons using pairwise average nucleotide identity analysis and the Genome-to-Genome Distance Calculator between strain NtB2T and L. lacti subsp.
30320546	6	1	theme	%	715:715	arg1	values					691:696	values	691:696	values of 81.0 and 23.2 %	691:715	tructae JCM 31125T gave values of 81.0 and 23.2 %, respectively.
30320546	9	2	theme	name	997:1000	arg1	sp					1026:1027	the name Lactococcus termiticola sp	993:1027	the name Lactococcus termiticola sp	993:1027	Based on the data presented, the isolate represents a novel species of the genus Lactococcus, for which the name Lactococcus termiticola sp.
30320546	5	3	theme	Genome-to-Genome	589:604	arg1	Calculator					615:624	the Genome-to-Genome Distance Calculator	585:624	the Genome-to-Genome Distance Calculator between strain NtB2T and L. lacti subsp	585:664	Furthermore, genomic comparisons using pairwise average nucleotide identity analysis and the Genome-to-Genome Distance Calculator between strain NtB2T and L. lacti subsp.
30320546	8	4	theme	peptidoglycan	827:839	arg1	A3α					866:868	A3α	866:868	A3α	866:868	The cell-wall peptidoglycan type of strain NtB2T was A3α, Lys-Gly-Ser-Ala2.
30320546	8	4	theme	peptidoglycan	827:839	arg1	type					841:844	The cell-wall peptidoglycan type	813:844	The cell-wall peptidoglycan type of strain NtB2T	813:860	The cell-wall peptidoglycan type of strain NtB2T was A3α, Lys-Gly-Ser-Ala2.
30320546	4	5	theme	brown	392:396	arg1	trout					398:402	brown trout	392:402	brown trout	392:402	tructae JCM 31125T isolated from brown trout, showing 93.2 and 81.0 % similarity in 16S rRNA gene and rpoB gene sequences, respectively.
30320546	8	6	theme	cell-wall	817:825	arg1	A3α					866:868	A3α	866:868	A3α	866:868	The cell-wall peptidoglycan type of strain NtB2T was A3α, Lys-Gly-Ser-Ala2.
30320546	8	6	theme	cell-wall	817:825	arg1	type					841:844	The cell-wall peptidoglycan type	813:844	The cell-wall peptidoglycan type of strain NtB2T	813:860	The cell-wall peptidoglycan type of strain NtB2T was A3α, Lys-Gly-Ser-Ala2.
30320546	2	7	theme	bacteria	143:150	arg1	strain					121:126	A strain	119:126	A strain	119:126	A strain of lactic acid bacteria, designated NtB2T, isolated from the gut of the wood-feeding higher termite Nasutitermes takasagoensis, was characterized genetically and phenotypically.
30320546	5	8	theme	pairwise	535:542	arg1	identity					563:570	pairwise average nucleotide identity	535:570	pairwise average nucleotide identity analysis	535:579	Furthermore, genomic comparisons using pairwise average nucleotide identity analysis and the Genome-to-Genome Distance Calculator between strain NtB2T and L. lacti subsp.
30320546	4	9	dep	similarity	429:438	arg1	%					427:427	%	427:427	%	427:427	tructae JCM 31125T isolated from brown trout, showing 93.2 and 81.0 % similarity in 16S rRNA gene and rpoB gene sequences, respectively.
30320546	3	10	theme	lacti	346:350	arg1	subsp					352:356	Lactococcus lacti subsp	334:356	Lactococcus lacti subsp	334:356	Strain NtB2T was related to Lactococcus lacti subsp.
30320546	2	11	theme	acid	138:141	arg1	bacteria					143:150	lactic acid bacteria	131:150	lactic acid bacteria	131:150	A strain of lactic acid bacteria, designated NtB2T, isolated from the gut of the wood-feeding higher termite Nasutitermes takasagoensis, was characterized genetically and phenotypically.
30320546	5	12	theme	strain	634:639	arg1	NtB2T					641:645	strain NtB2T	634:645	strain NtB2T	634:645	Furthermore, genomic comparisons using pairwise average nucleotide identity analysis and the Genome-to-Genome Distance Calculator between strain NtB2T and L. lacti subsp.
30320546	2	13	theme	lactic	131:136	arg1	bacteria					143:150	lactic acid bacteria	131:150	lactic acid bacteria	131:150	A strain of lactic acid bacteria, designated NtB2T, isolated from the gut of the wood-feeding higher termite Nasutitermes takasagoensis, was characterized genetically and phenotypically.
30320546	5	14	theme	identity	563:570	arg1	analysis					572:579	pairwise average nucleotide identity analysis	535:579	pairwise average nucleotide identity analysis	535:579	Furthermore, genomic comparisons using pairwise average nucleotide identity analysis and the Genome-to-Genome Distance Calculator between strain NtB2T and L. lacti subsp.
30320546	8	15	theme	NtB2T	856:860	arg1	A3α					866:868	A3α	866:868	A3α	866:868	The cell-wall peptidoglycan type of strain NtB2T was A3α, Lys-Gly-Ser-Ala2.
30320546	8	15	theme	NtB2T	856:860	arg1	type					841:844	The cell-wall peptidoglycan type	813:844	The cell-wall peptidoglycan type of strain NtB2T	813:860	The cell-wall peptidoglycan type of strain NtB2T was A3α, Lys-Gly-Ser-Ala2.
30320546	0	16	theme	termiticola	12:22	arg1	sp					24:25	Lactococcus termiticola sp	0:25	Lactococcus termiticola sp.	0:26	Lactococcus termiticola sp.
30320546	4	17	theme	rpoB	461:464	arg1	sequences					471:479	16S rRNA gene and rpoB gene sequences	443:479	sequences	471:479	tructae JCM 31125T isolated from brown trout, showing 93.2 and 81.0 % similarity in 16S rRNA gene and rpoB gene sequences, respectively.
30320546	4	18	theme	JCM	367:369	arg1	31125T					371:376	tructae JCM 31125T	359:376	tructae JCM 31125T	359:376	tructae JCM 31125T isolated from brown trout, showing 93.2 and 81.0 % similarity in 16S rRNA gene and rpoB gene sequences, respectively.
30320546	1	19	theme	wood-feeding	63:74	arg1	takasagoensis					104:116	the wood-feeding higher termite Nasutitermes takasagoensis	59:116	the wood-feeding higher termite Nasutitermes takasagoensis	59:116	nov., isolated from the gut of the wood-feeding higher termite Nasutitermes takasagoensis.
30320546	8	20	theme	strain	849:854	arg1	NtB2T					856:860	strain NtB2T	849:860	strain NtB2T	849:860	The cell-wall peptidoglycan type of strain NtB2T was A3α, Lys-Gly-Ser-Ala2.
30320546	2	21	attach	isolated	171:178	arg2	strain					121:126	A strain	119:126	A strain	119:126	A strain of lactic acid bacteria, designated NtB2T, isolated from the gut of the wood-feeding higher termite Nasutitermes takasagoensis, was characterized genetically and phenotypically.
30320546	2	21	attach	isolated	171:178	arg1	gut					189:191	the gut	185:191	the gut of the wood-feeding higher termite Nasutitermes takasagoensis	185:253	A strain of lactic acid bacteria, designated NtB2T, isolated from the gut of the wood-feeding higher termite Nasutitermes takasagoensis, was characterized genetically and phenotypically.
30320546	0	22	theme	Lactococcus	0:10	arg1	sp					24:25	Lactococcus termiticola sp	0:25	Lactococcus termiticola sp.	0:26	Lactococcus termiticola sp.
30320546	5	23	theme	L.	651:652	arg1	subsp					660:664	L. lacti subsp	651:664	L. lacti subsp	651:664	Furthermore, genomic comparisons using pairwise average nucleotide identity analysis and the Genome-to-Genome Distance Calculator between strain NtB2T and L. lacti subsp.
30320546	1	24	theme	higher	76:81	arg1	takasagoensis					104:116	the wood-feeding higher termite Nasutitermes takasagoensis	59:116	the wood-feeding higher termite Nasutitermes takasagoensis	59:116	nov., isolated from the gut of the wood-feeding higher termite Nasutitermes takasagoensis.
30320546	11	25	theme	=JCM	1074:1077	arg1	NtB2T					1067:1071	NtB2T	1067:1071	NtB2T (=JCM 32569T=DSM 107259T)	1067:1097	The type strain is NtB2T (=JCM 32569T=DSM 107259T).
30320546	11	25	theme	=JCM	1074:1077	arg1	107259T					1090:1096	=JCM 32569T=DSM 107259T	1074:1096	=JCM 32569T=DSM 107259T	1074:1096	The type strain is NtB2T (=JCM 32569T=DSM 107259T).
30320546	1	26	theme	termite	83:89	arg1	takasagoensis					104:116	the wood-feeding higher termite Nasutitermes takasagoensis	59:116	the wood-feeding higher termite Nasutitermes takasagoensis	59:116	nov., isolated from the gut of the wood-feeding higher termite Nasutitermes takasagoensis.
30320546	9	27	theme	novel	943:947	arg1	species					949:955	a novel species	941:955	a novel species	941:955	Based on the data presented, the isolate represents a novel species of the genus Lactococcus, for which the name Lactococcus termiticola sp.
30320546	5	28	theme	average	544:550	arg1	identity					563:570	pairwise average nucleotide identity	535:570	pairwise average nucleotide identity analysis	535:579	Furthermore, genomic comparisons using pairwise average nucleotide identity analysis and the Genome-to-Genome Distance Calculator between strain NtB2T and L. lacti subsp.
30320546	1	29	theme	Nasutitermes	91:102	arg1	takasagoensis					104:116	the wood-feeding higher termite Nasutitermes takasagoensis	59:116	the wood-feeding higher termite Nasutitermes takasagoensis	59:116	nov., isolated from the gut of the wood-feeding higher termite Nasutitermes takasagoensis.
30320546	11	30	theme	type	1052:1055	arg1	NtB2T					1067:1071	NtB2T	1067:1071	NtB2T (=JCM 32569T=DSM 107259T)	1067:1097	The type strain is NtB2T (=JCM 32569T=DSM 107259T).
30320546	11	30	theme	type	1052:1055	arg1	strain					1057:1062	The type strain	1048:1062	The type strain	1048:1062	The type strain is NtB2T (=JCM 32569T=DSM 107259T).
30320546	9	31	theme	Lactococcus	1002:1012	arg1	sp					1026:1027	the name Lactococcus termiticola sp	993:1027	the name Lactococcus termiticola sp	993:1027	Based on the data presented, the isolate represents a novel species of the genus Lactococcus, for which the name Lactococcus termiticola sp.
30320546	6	32	theme	JCM	675:677	arg1	31125T					679:684	tructae JCM 31125T	667:684	tructae JCM 31125T	667:684	tructae JCM 31125T gave values of 81.0 and 23.2 %, respectively.
30320546	9	33	theme	Lactococcus	970:980	arg1	species					949:955	a novel species	941:955	a novel species	941:955	Based on the data presented, the isolate represents a novel species of the genus Lactococcus, for which the name Lactococcus termiticola sp.
30320546	7	34	theme	strain	771:776	arg1	NtB2T					778:782	strain NtB2T	771:782	strain NtB2T	771:782	Major cellular fatty acids produced by strain NtB2T were C18 : 1ω9c and C16 : 0.
30320546	4	35	from	similarity	429:438	arg1	gene					452:455	16S rRNA gene and rpoB gene sequences	443:479	gene	452:455	tructae JCM 31125T isolated from brown trout, showing 93.2 and 81.0 % similarity in 16S rRNA gene and rpoB gene sequences, respectively.
30320546	4	35	from	similarity	429:438	arg1	sequences					471:479	16S rRNA gene and rpoB gene sequences	443:479	sequences	471:479	tructae JCM 31125T isolated from brown trout, showing 93.2 and 81.0 % similarity in 16S rRNA gene and rpoB gene sequences, respectively.
30320546	4	36	theme	rRNA	447:450	arg1	gene					452:455	16S rRNA gene and rpoB gene sequences	443:479	gene	452:455	tructae JCM 31125T isolated from brown trout, showing 93.2 and 81.0 % similarity in 16S rRNA gene and rpoB gene sequences, respectively.
30320546	2	37	theme	wood-feeding	200:211	arg1	takasagoensis					241:253	the wood-feeding higher termite Nasutitermes takasagoensis	196:253	the wood-feeding higher termite Nasutitermes takasagoensis	196:253	A strain of lactic acid bacteria, designated NtB2T, isolated from the gut of the wood-feeding higher termite Nasutitermes takasagoensis, was characterized genetically and phenotypically.
30320546	2	38	dep	wood-feeding	200:211	arg1	higher					213:218	higher	213:218	higher	213:218	A strain of lactic acid bacteria, designated NtB2T, isolated from the gut of the wood-feeding higher termite Nasutitermes takasagoensis, was characterized genetically and phenotypically.
30320546	3	39	theme	Strain	306:311	arg1	NtB2T					313:317	Strain NtB2T	306:317	Strain NtB2T	306:317	Strain NtB2T was related to Lactococcus lacti subsp.
30320546	4	40	theme	16S	443:445	arg1	gene					452:455	16S rRNA gene and rpoB gene sequences	443:479	gene	452:455	tructae JCM 31125T isolated from brown trout, showing 93.2 and 81.0 % similarity in 16S rRNA gene and rpoB gene sequences, respectively.
30320546	4	41	theme	gene	466:469	arg1	sequences					471:479	16S rRNA gene and rpoB gene sequences	443:479	sequences	471:479	tructae JCM 31125T isolated from brown trout, showing 93.2 and 81.0 % similarity in 16S rRNA gene and rpoB gene sequences, respectively.
30320546	1	42	theme	takasagoensis	104:116	arg1	gut					52:54	the gut	48:54	the gut of the wood-feeding higher termite Nasutitermes takasagoensis	48:116	nov., isolated from the gut of the wood-feeding higher termite Nasutitermes takasagoensis.
30320546	9	43	theme	genus	964:968	arg1	Lactococcus					970:980	the genus Lactococcus	960:980	the genus Lactococcus	960:980	Based on the data presented, the isolate represents a novel species of the genus Lactococcus, for which the name Lactococcus termiticola sp.
30320546	4	44	theme	tructae	359:365	arg1	31125T					371:376	tructae JCM 31125T	359:376	tructae JCM 31125T	359:376	tructae JCM 31125T isolated from brown trout, showing 93.2 and 81.0 % similarity in 16S rRNA gene and rpoB gene sequences, respectively.
30320546	6	45	theme	tructae	667:673	arg1	31125T					679:684	tructae JCM 31125T	667:684	tructae JCM 31125T	667:684	tructae JCM 31125T gave values of 81.0 and 23.2 %, respectively.
30320546	11	46	theme	32569T=DSM	1079:1088	arg1	NtB2T					1067:1071	NtB2T	1067:1071	NtB2T (=JCM 32569T=DSM 107259T)	1067:1097	The type strain is NtB2T (=JCM 32569T=DSM 107259T).
30320546	11	46	theme	32569T=DSM	1079:1088	arg1	107259T					1090:1096	=JCM 32569T=DSM 107259T	1074:1096	=JCM 32569T=DSM 107259T	1074:1096	The type strain is NtB2T (=JCM 32569T=DSM 107259T).
30320546	9	47	dep	Lactococcus	1002:1012	arg1	termiticola					1014:1024	termiticola	1014:1024	termiticola	1014:1024	Based on the data presented, the isolate represents a novel species of the genus Lactococcus, for which the name Lactococcus termiticola sp.
30320546	5	48	theme	Distance	606:613	arg1	Calculator					615:624	the Genome-to-Genome Distance Calculator	585:624	the Genome-to-Genome Distance Calculator between strain NtB2T and L. lacti subsp	585:664	Furthermore, genomic comparisons using pairwise average nucleotide identity analysis and the Genome-to-Genome Distance Calculator between strain NtB2T and L. lacti subsp.
30320546	4	49	dep	%	427:427	arg1	81.0 					422:426	81.0 	422:426	81.0 	422:426	tructae JCM 31125T isolated from brown trout, showing 93.2 and 81.0 % similarity in 16S rRNA gene and rpoB gene sequences, respectively.
30320546	4	49	dep	%	427:427	arg1	93.2					413:416	93.2	413:416	93.2	413:416	tructae JCM 31125T isolated from brown trout, showing 93.2 and 81.0 % similarity in 16S rRNA gene and rpoB gene sequences, respectively.
30320546	5	50	theme	nucleotide	552:561	arg1	identity					563:570	pairwise average nucleotide identity	535:570	pairwise average nucleotide identity analysis	535:579	Furthermore, genomic comparisons using pairwise average nucleotide identity analysis and the Genome-to-Genome Distance Calculator between strain NtB2T and L. lacti subsp.
30320546	2	51	theme	takasagoensis	241:253	arg1	gut					189:191	the gut	185:191	the gut of the wood-feeding higher termite Nasutitermes takasagoensis	185:253	A strain of lactic acid bacteria, designated NtB2T, isolated from the gut of the wood-feeding higher termite Nasutitermes takasagoensis, was characterized genetically and phenotypically.
30320546	2	52	theme	Nasutitermes	228:239	arg1	takasagoensis					241:253	the wood-feeding higher termite Nasutitermes takasagoensis	196:253	the wood-feeding higher termite Nasutitermes takasagoensis	196:253	A strain of lactic acid bacteria, designated NtB2T, isolated from the gut of the wood-feeding higher termite Nasutitermes takasagoensis, was characterized genetically and phenotypically.
30320546	7	53	theme	Major	732:736	arg1	acids					753:757	Major cellular fatty acids	732:757	Major cellular fatty acids produced by strain NtB2T	732:782	Major cellular fatty acids produced by strain NtB2T were C18 : 1ω9c and C16 : 0.
30320546	3	54	theme	Lactococcus	334:344	arg1	subsp					352:356	Lactococcus lacti subsp	334:356	Lactococcus lacti subsp	334:356	Strain NtB2T was related to Lactococcus lacti subsp.
30320546	2	55	theme	termite	220:226	arg1	takasagoensis					241:253	the wood-feeding higher termite Nasutitermes takasagoensis	196:253	the wood-feeding higher termite Nasutitermes takasagoensis	196:253	A strain of lactic acid bacteria, designated NtB2T, isolated from the gut of the wood-feeding higher termite Nasutitermes takasagoensis, was characterized genetically and phenotypically.
30320546	5	56	theme	genomic	509:515	arg1	comparisons					517:527	genomic comparisons	509:527	genomic comparisons	509:527	Furthermore, genomic comparisons using pairwise average nucleotide identity analysis and the Genome-to-Genome Distance Calculator between strain NtB2T and L. lacti subsp.
30320546	7	57	theme	fatty	747:751	arg1	acids					753:757	Major cellular fatty acids	732:757	Major cellular fatty acids produced by strain NtB2T	732:782	Major cellular fatty acids produced by strain NtB2T were C18 : 1ω9c and C16 : 0.
30320546	7	58	theme	cellular	738:745	arg1	acids					753:757	Major cellular fatty acids	732:757	Major cellular fatty acids produced by strain NtB2T	732:782	Major cellular fatty acids produced by strain NtB2T were C18 : 1ω9c and C16 : 0.
30616345	0	0	theme	Cells	99:103	arg1	Viability					51:59	Viability	51:59	Viability	51:59	Membrane-Binding Adhesive Particulates Enhance the Viability and Paracrine Function of Mesenchymal Cells for Cell-Based Therapy.
30616345	0	0	theme	Cells	99:103	arg1	Function					75:82	Paracrine Function	65:82	Paracrine Function	65:82	Membrane-Binding Adhesive Particulates Enhance the Viability and Paracrine Function of Mesenchymal Cells for Cell-Based Therapy.
30616345	4	1	link	adipose-derived	763:777	arg1	Ad-MSCs					806:812	Ad-MSCs	806:812	Ad-MSCs	806:812	The adhesive particulates were used to coat the surface of adipose-derived mesenchymal stromal cells (Ad-MSCs) and studied in the MSC spheroid culture.
30616345	4	1	link	adipose-derived	763:777	arg1	cells					799:803	adipose-derived mesenchymal stromal cells	763:803	adipose-derived mesenchymal stromal cells (Ad-MSCs)	763:813	The adhesive particulates were used to coat the surface of adipose-derived mesenchymal stromal cells (Ad-MSCs) and studied in the MSC spheroid culture.
30616345	0	2	theme	Mesenchymal	87:97	arg1	Cells					99:103	Mesenchymal Cells	87:103	Mesenchymal Cells	87:103	Membrane-Binding Adhesive Particulates Enhance the Viability and Paracrine Function of Mesenchymal Cells for Cell-Based Therapy.
30616345	6	3	theme	PAMAM-IKVAV/HA	1169:1182	arg1	particulates					1194:1205	PAMAM-IKVAV/HA composite particulates	1169:1205	PAMAM-IKVAV/HA composite particulates in a rat myocardial infarction model	1169:1242	The transplantation of the Ad-MSCs primed with PAMAM-IKVAV/HA composite particulates in a rat myocardial infarction model further demonstrated the beneficial effects of membrane-binding materials on improving the cell retention and tissue angiogenesis.
30616345	5	4	from	expression	993:1002	arg1	MSCs					1037:1040	MSCs	1037:1040	MSCs	1037:1040	The analysis showed that the adhesive complexes formed via PAMAM conjugates and HA significantly promoted the proliferation and the gene expression of pro-angiogenesis cytokines in MSCs; the production of anti-inflammatory miRNAs in exosomes could also be elevated.
30616345	0	5	theme	Cell-Based	109:118	arg1	Therapy					120:126	Cell-Based Therapy	109:126	Cell-Based Therapy	109:126	Membrane-Binding Adhesive Particulates Enhance the Viability and Paracrine Function of Mesenchymal Cells for Cell-Based Therapy.
30616345	6	6	theme	tissue	1354:1359	arg1	angiogenesis					1361:1372	tissue angiogenesis	1354:1372	tissue angiogenesis	1354:1372	The transplantation of the Ad-MSCs primed with PAMAM-IKVAV/HA composite particulates in a rat myocardial infarction model further demonstrated the beneficial effects of membrane-binding materials on improving the cell retention and tissue angiogenesis.
30616345	0	7	dep	Viability	51:59	arg1	the					47:49	the	47:49	the	47:49	Membrane-Binding Adhesive Particulates Enhance the Viability and Paracrine Function of Mesenchymal Cells for Cell-Based Therapy.
30616345	5	8	theme	PAMAM	915:919	arg1	conjugates					921:930	PAMAM conjugates	915:930	PAMAM conjugates	915:930	The analysis showed that the adhesive complexes formed via PAMAM conjugates and HA significantly promoted the proliferation and the gene expression of pro-angiogenesis cytokines in MSCs; the production of anti-inflammatory miRNAs in exosomes could also be elevated.
30616345	5	9	theme	gene	988:991	arg1	expression					993:1002	the gene expression	984:1002	the gene expression of pro-angiogenesis cytokines in MSCs	984:1040	The analysis showed that the adhesive complexes formed via PAMAM conjugates and HA significantly promoted the proliferation and the gene expression of pro-angiogenesis cytokines in MSCs; the production of anti-inflammatory miRNAs in exosomes could also be elevated.
30616345	6	10	theme	rat	1212:1214	arg1	infarction					1227:1236	a rat myocardial infarction	1210:1236	a rat myocardial infarction model	1210:1242	The transplantation of the Ad-MSCs primed with PAMAM-IKVAV/HA composite particulates in a rat myocardial infarction model further demonstrated the beneficial effects of membrane-binding materials on improving the cell retention and tissue angiogenesis.
30616345	4	11	theme	cells	799:803	arg1	surface					752:758	the surface	748:758	the surface of adipose-derived mesenchymal stromal cells (Ad-MSCs)	748:813	The adhesive particulates were used to coat the surface of adipose-derived mesenchymal stromal cells (Ad-MSCs) and studied in the MSC spheroid culture.
30616345	1	12	theme	cell-material	159:171	arg1	interactions					173:184	the fundamental cell-material interactions	143:184	the fundamental cell-material interactions	143:184	Understanding the fundamental cell-material interactions is essential to designing functional materials for biomedical applications.
30616345	6	13	theme	composite	1184:1192	arg1	particulates					1194:1205	PAMAM-IKVAV/HA composite particulates	1169:1205	PAMAM-IKVAV/HA composite particulates in a rat myocardial infarction model	1169:1242	The transplantation of the Ad-MSCs primed with PAMAM-IKVAV/HA composite particulates in a rat myocardial infarction model further demonstrated the beneficial effects of membrane-binding materials on improving the cell retention and tissue angiogenesis.
30616345	5	14	theme	pro-angiogenesis	1007:1022	arg1	cytokines					1024:1032	pro-angiogenesis cytokines	1007:1032	pro-angiogenesis cytokines	1007:1032	The analysis showed that the adhesive complexes formed via PAMAM conjugates and HA significantly promoted the proliferation and the gene expression of pro-angiogenesis cytokines in MSCs; the production of anti-inflammatory miRNAs in exosomes could also be elevated.
30616345	7	15	theme	membrane-binding	1395:1410	arg1	materials					1421:1429	membrane-binding adhesive materials	1395:1429	membrane-binding adhesive materials	1395:1429	The new function of membrane-binding adhesive materials potentially provides useful ways to improve cell-based therapy.
30616345	1	16	theme	designing	202:210	arg1	materials					223:231	designing functional materials	202:231	designing functional materials for biomedical applications	202:259	Understanding the fundamental cell-material interactions is essential to designing functional materials for biomedical applications.
30616345	6	17	theme	cell	1335:1338	arg1	retention					1340:1348	the cell retention	1331:1348	the cell retention	1331:1348	The transplantation of the Ad-MSCs primed with PAMAM-IKVAV/HA composite particulates in a rat myocardial infarction model further demonstrated the beneficial effects of membrane-binding materials on improving the cell retention and tissue angiogenesis.
30616345	5	18	theme	cytokines	1024:1032	arg1	proliferation					966:978	the proliferation	962:978	the proliferation	962:978	The analysis showed that the adhesive complexes formed via PAMAM conjugates and HA significantly promoted the proliferation and the gene expression of pro-angiogenesis cytokines in MSCs; the production of anti-inflammatory miRNAs in exosomes could also be elevated.
30616345	5	18	theme	cytokines	1024:1032	arg1	expression					993:1002	the gene expression	984:1002	the gene expression of pro-angiogenesis cytokines in MSCs	984:1040	The analysis showed that the adhesive complexes formed via PAMAM conjugates and HA significantly promoted the proliferation and the gene expression of pro-angiogenesis cytokines in MSCs; the production of anti-inflammatory miRNAs in exosomes could also be elevated.
30616345	1	19	theme	functional	212:221	arg1	materials					223:231	designing functional materials	202:231	designing functional materials for biomedical applications	202:259	Understanding the fundamental cell-material interactions is essential to designing functional materials for biomedical applications.
30616345	0	20	theme	Adhesive	17:24	arg1	Particulates					26:37	Membrane-Binding Adhesive Particulates	0:37	Membrane-Binding Adhesive Particulates	0:37	Membrane-Binding Adhesive Particulates Enhance the Viability and Paracrine Function of Mesenchymal Cells for Cell-Based Therapy.
30616345	4	21	used	used	735:738	arg2	particulates					717:728	The adhesive particulates	704:728	The adhesive particulates	704:728	The adhesive particulates were used to coat the surface of adipose-derived mesenchymal stromal cells (Ad-MSCs) and studied in the MSC spheroid culture.
30616345	4	22	theme	adipose-derived	763:777	arg1	Ad-MSCs					806:812	Ad-MSCs	806:812	Ad-MSCs	806:812	The adhesive particulates were used to coat the surface of adipose-derived mesenchymal stromal cells (Ad-MSCs) and studied in the MSC spheroid culture.
30616345	4	22	theme	adipose-derived	763:777	arg1	cells					799:803	adipose-derived mesenchymal stromal cells	763:803	adipose-derived mesenchymal stromal cells (Ad-MSCs)	763:813	The adhesive particulates were used to coat the surface of adipose-derived mesenchymal stromal cells (Ad-MSCs) and studied in the MSC spheroid culture.
30616345	2	23	theme	biomaterials	424:435	arg1	property					412:419	the inherent property	399:419	the inherent property of biomaterials to modulate the therapeutic function of MSCs	399:480	Although mesenchymal stromal cells (MSCs) are known to secrete cytokines and exosomes that are effective to treat degenerative diseases, the inherent property of biomaterials to modulate the therapeutic function of MSCs remains to be investigated.
30616345	0	24	theme	Membrane-Binding	0:15	arg1	Particulates					26:37	Membrane-Binding Adhesive Particulates	0:37	Membrane-Binding Adhesive Particulates	0:37	Membrane-Binding Adhesive Particulates Enhance the Viability and Paracrine Function of Mesenchymal Cells for Cell-Based Therapy.
30616345	5	25	from	production	1047:1056	arg1	exosomes					1089:1096	exosomes	1089:1096	exosomes	1089:1096	The analysis showed that the adhesive complexes formed via PAMAM conjugates and HA significantly promoted the proliferation and the gene expression of pro-angiogenesis cytokines in MSCs; the production of anti-inflammatory miRNAs in exosomes could also be elevated.
30616345	7	26	theme	adhesive	1412:1419	arg1	materials					1421:1429	membrane-binding adhesive materials	1395:1429	membrane-binding adhesive materials	1395:1429	The new function of membrane-binding adhesive materials potentially provides useful ways to improve cell-based therapy.
30616345	5	27	theme	miRNAs	1079:1084	arg1	production					1047:1056	the production	1043:1056	the production of anti-inflammatory miRNAs in exosomes	1043:1096	The analysis showed that the adhesive complexes formed via PAMAM conjugates and HA significantly promoted the proliferation and the gene expression of pro-angiogenesis cytokines in MSCs; the production of anti-inflammatory miRNAs in exosomes could also be elevated.
30616345	4	28	theme	stromal	791:797	arg1	Ad-MSCs					806:812	Ad-MSCs	806:812	Ad-MSCs	806:812	The adhesive particulates were used to coat the surface of adipose-derived mesenchymal stromal cells (Ad-MSCs) and studied in the MSC spheroid culture.
30616345	4	28	theme	stromal	791:797	arg1	cells					799:803	adipose-derived mesenchymal stromal cells	763:803	adipose-derived mesenchymal stromal cells (Ad-MSCs)	763:813	The adhesive particulates were used to coat the surface of adipose-derived mesenchymal stromal cells (Ad-MSCs) and studied in the MSC spheroid culture.
30616345	6	29	from	particulates	1194:1205	arg1	model					1238:1242	a rat myocardial infarction model	1210:1242	a rat myocardial infarction model	1210:1242	The transplantation of the Ad-MSCs primed with PAMAM-IKVAV/HA composite particulates in a rat myocardial infarction model further demonstrated the beneficial effects of membrane-binding materials on improving the cell retention and tissue angiogenesis.
30616345	1	30	theme	biomedical	237:246	arg1	applications					248:259	biomedical applications	237:259	biomedical applications	237:259	Understanding the fundamental cell-material interactions is essential to designing functional materials for biomedical applications.
30616345	2	31	theme	MSCs	477:480	arg1	function					465:472	the therapeutic function	449:472	the therapeutic function of MSCs	449:480	Although mesenchymal stromal cells (MSCs) are known to secrete cytokines and exosomes that are effective to treat degenerative diseases, the inherent property of biomaterials to modulate the therapeutic function of MSCs remains to be investigated.
30616345	6	32	theme	infarction	1227:1236	arg1	model					1238:1242	a rat myocardial infarction model	1210:1242	a rat myocardial infarction model	1210:1242	The transplantation of the Ad-MSCs primed with PAMAM-IKVAV/HA composite particulates in a rat myocardial infarction model further demonstrated the beneficial effects of membrane-binding materials on improving the cell retention and tissue angiogenesis.
30616345	4	33	theme	mesenchymal	779:789	arg1	Ad-MSCs					806:812	Ad-MSCs	806:812	Ad-MSCs	806:812	The adhesive particulates were used to coat the surface of adipose-derived mesenchymal stromal cells (Ad-MSCs) and studied in the MSC spheroid culture.
30616345	4	33	theme	mesenchymal	779:789	arg1	cells					799:803	adipose-derived mesenchymal stromal cells	763:803	adipose-derived mesenchymal stromal cells (Ad-MSCs)	763:813	The adhesive particulates were used to coat the surface of adipose-derived mesenchymal stromal cells (Ad-MSCs) and studied in the MSC spheroid culture.
30616345	5	34	theme	anti-inflammatory	1061:1077	arg1	miRNAs					1079:1084	anti-inflammatory miRNAs	1061:1084	anti-inflammatory miRNAs	1061:1084	The analysis showed that the adhesive complexes formed via PAMAM conjugates and HA significantly promoted the proliferation and the gene expression of pro-angiogenesis cytokines in MSCs; the production of anti-inflammatory miRNAs in exosomes could also be elevated.
30616345	3	35	theme	adhesive	544:551	arg1	conjugate					553:561	a multivalent cell-membrane adhesive conjugate	516:561	a multivalent cell-membrane adhesive conjugate	516:561	Here, a multivalent cell-membrane adhesive conjugate was generated through polyamindoamine (PAMAM) and an oligopeptide, IKVAV, and the conjugate was further complexed with hyaluronic acid (HA).
30616345	6	36	theme	membrane-binding	1291:1306	arg1	materials					1308:1316	membrane-binding materials	1291:1316	membrane-binding materials	1291:1316	The transplantation of the Ad-MSCs primed with PAMAM-IKVAV/HA composite particulates in a rat myocardial infarction model further demonstrated the beneficial effects of membrane-binding materials on improving the cell retention and tissue angiogenesis.
30616345	4	37	theme	spheroid	838:845	arg1	culture					847:853	the MSC spheroid culture	830:853	the MSC spheroid culture	830:853	The adhesive particulates were used to coat the surface of adipose-derived mesenchymal stromal cells (Ad-MSCs) and studied in the MSC spheroid culture.
30616345	2	38	theme	therapeutic	453:463	arg1	function					465:472	the therapeutic function	449:472	the therapeutic function of MSCs	449:480	Although mesenchymal stromal cells (MSCs) are known to secrete cytokines and exosomes that are effective to treat degenerative diseases, the inherent property of biomaterials to modulate the therapeutic function of MSCs remains to be investigated.
30616345	7	39	theme	materials	1421:1429	arg1	function					1383:1390	The new function	1375:1390	The new function of membrane-binding adhesive materials	1375:1429	The new function of membrane-binding adhesive materials potentially provides useful ways to improve cell-based therapy.
30616345	4	40	theme	MSC	834:836	arg1	culture					847:853	the MSC spheroid culture	830:853	the MSC spheroid culture	830:853	The adhesive particulates were used to coat the surface of adipose-derived mesenchymal stromal cells (Ad-MSCs) and studied in the MSC spheroid culture.
30616345	7	41	theme	cell-based	1475:1484	arg1	therapy					1486:1492	cell-based therapy	1475:1492	cell-based therapy	1475:1492	The new function of membrane-binding adhesive materials potentially provides useful ways to improve cell-based therapy.
30616345	7	42	theme	new	1379:1381	arg1	function					1383:1390	The new function	1375:1390	The new function of membrane-binding adhesive materials	1375:1429	The new function of membrane-binding adhesive materials potentially provides useful ways to improve cell-based therapy.
30616345	0	43	theme	Paracrine	65:73	arg1	Function					75:82	Paracrine Function	65:82	Paracrine Function	65:82	Membrane-Binding Adhesive Particulates Enhance the Viability and Paracrine Function of Mesenchymal Cells for Cell-Based Therapy.
30616345	3	44	theme	hyaluronic	682:691	arg1	acid					693:696	hyaluronic acid	682:696	hyaluronic acid (HA)	682:701	Here, a multivalent cell-membrane adhesive conjugate was generated through polyamindoamine (PAMAM) and an oligopeptide, IKVAV, and the conjugate was further complexed with hyaluronic acid (HA).
30616345	3	44	theme	hyaluronic	682:691	arg1	HA					699:700	HA	699:700	HA	699:700	Here, a multivalent cell-membrane adhesive conjugate was generated through polyamindoamine (PAMAM) and an oligopeptide, IKVAV, and the conjugate was further complexed with hyaluronic acid (HA).
30616345	2	45	theme	stromal	283:289	arg1	cells					291:295	mesenchymal stromal cells	271:295	mesenchymal stromal cells (MSCs)	271:302	Although mesenchymal stromal cells (MSCs) are known to secrete cytokines and exosomes that are effective to treat degenerative diseases, the inherent property of biomaterials to modulate the therapeutic function of MSCs remains to be investigated.
30616345	2	45	theme	stromal	283:289	arg1	MSCs					298:301	MSCs	298:301	MSCs	298:301	Although mesenchymal stromal cells (MSCs) are known to secrete cytokines and exosomes that are effective to treat degenerative diseases, the inherent property of biomaterials to modulate the therapeutic function of MSCs remains to be investigated.
30616345	3	46	theme	cell-membrane	530:542	arg1	conjugate					553:561	a multivalent cell-membrane adhesive conjugate	516:561	a multivalent cell-membrane adhesive conjugate	516:561	Here, a multivalent cell-membrane adhesive conjugate was generated through polyamindoamine (PAMAM) and an oligopeptide, IKVAV, and the conjugate was further complexed with hyaluronic acid (HA).
30616345	6	47	theme	materials	1308:1316	arg1	effects					1280:1286	the beneficial effects	1265:1286	the beneficial effects of membrane-binding materials on improving the cell retention and tissue angiogenesis	1265:1372	The transplantation of the Ad-MSCs primed with PAMAM-IKVAV/HA composite particulates in a rat myocardial infarction model further demonstrated the beneficial effects of membrane-binding materials on improving the cell retention and tissue angiogenesis.
30616345	2	48	theme	mesenchymal	271:281	arg1	cells					291:295	mesenchymal stromal cells	271:295	mesenchymal stromal cells (MSCs)	271:302	Although mesenchymal stromal cells (MSCs) are known to secrete cytokines and exosomes that are effective to treat degenerative diseases, the inherent property of biomaterials to modulate the therapeutic function of MSCs remains to be investigated.
30616345	2	48	theme	mesenchymal	271:281	arg1	MSCs					298:301	MSCs	298:301	MSCs	298:301	Although mesenchymal stromal cells (MSCs) are known to secrete cytokines and exosomes that are effective to treat degenerative diseases, the inherent property of biomaterials to modulate the therapeutic function of MSCs remains to be investigated.
30616345	2	49	theme	degenerative	376:387	arg1	diseases					389:396	degenerative diseases	376:396	degenerative diseases	376:396	Although mesenchymal stromal cells (MSCs) are known to secrete cytokines and exosomes that are effective to treat degenerative diseases, the inherent property of biomaterials to modulate the therapeutic function of MSCs remains to be investigated.
30616345	4	50	theme	adhesive	708:715	arg1	particulates					717:728	The adhesive particulates	704:728	The adhesive particulates	704:728	The adhesive particulates were used to coat the surface of adipose-derived mesenchymal stromal cells (Ad-MSCs) and studied in the MSC spheroid culture.
30616345	1	51	theme	fundamental	147:157	arg1	interactions					173:184	the fundamental cell-material interactions	143:184	the fundamental cell-material interactions	143:184	Understanding the fundamental cell-material interactions is essential to designing functional materials for biomedical applications.
30616345	6	52	theme	myocardial	1216:1225	arg1	infarction					1227:1236	a rat myocardial infarction	1210:1236	a rat myocardial infarction model	1210:1242	The transplantation of the Ad-MSCs primed with PAMAM-IKVAV/HA composite particulates in a rat myocardial infarction model further demonstrated the beneficial effects of membrane-binding materials on improving the cell retention and tissue angiogenesis.
30616345	6	53	theme	beneficial	1269:1278	arg1	effects					1280:1286	the beneficial effects	1265:1286	the beneficial effects of membrane-binding materials on improving the cell retention and tissue angiogenesis	1265:1372	The transplantation of the Ad-MSCs primed with PAMAM-IKVAV/HA composite particulates in a rat myocardial infarction model further demonstrated the beneficial effects of membrane-binding materials on improving the cell retention and tissue angiogenesis.
30616345	3	54	theme	multivalent	518:528	arg1	conjugate					553:561	a multivalent cell-membrane adhesive conjugate	516:561	a multivalent cell-membrane adhesive conjugate	516:561	Here, a multivalent cell-membrane adhesive conjugate was generated through polyamindoamine (PAMAM) and an oligopeptide, IKVAV, and the conjugate was further complexed with hyaluronic acid (HA).
30616345	5	55	from	proliferation	966:978	arg1	MSCs					1037:1040	MSCs	1037:1040	MSCs	1037:1040	The analysis showed that the adhesive complexes formed via PAMAM conjugates and HA significantly promoted the proliferation and the gene expression of pro-angiogenesis cytokines in MSCs; the production of anti-inflammatory miRNAs in exosomes could also be elevated.
30616345	6	56	theme	Ad-MSCs	1149:1155	arg1	transplantation					1126:1140	The transplantation	1122:1140	The transplantation of the Ad-MSCs primed with PAMAM-IKVAV/HA composite particulates in a rat myocardial infarction model	1122:1242	The transplantation of the Ad-MSCs primed with PAMAM-IKVAV/HA composite particulates in a rat myocardial infarction model further demonstrated the beneficial effects of membrane-binding materials on improving the cell retention and tissue angiogenesis.
30616345	5	57	theme	adhesive	885:892	arg1	complexes					894:902	the adhesive complexes	881:902	the adhesive complexes formed via PAMAM conjugates and HA	881:937	The analysis showed that the adhesive complexes formed via PAMAM conjugates and HA significantly promoted the proliferation and the gene expression of pro-angiogenesis cytokines in MSCs; the production of anti-inflammatory miRNAs in exosomes could also be elevated.
30616345	7	58	theme	useful	1452:1457	arg1	ways					1459:1462	useful ways	1452:1462	useful ways	1452:1462	The new function of membrane-binding adhesive materials potentially provides useful ways to improve cell-based therapy.
30616345	2	59	theme	inherent	403:410	arg1	property					412:419	the inherent property	399:419	the inherent property of biomaterials to modulate the therapeutic function of MSCs	399:480	Although mesenchymal stromal cells (MSCs) are known to secrete cytokines and exosomes that are effective to treat degenerative diseases, the inherent property of biomaterials to modulate the therapeutic function of MSCs remains to be investigated.
31278319	10	0	theme	salivary	1099:1106	arg1	output					1116:1121	salivary protein output	1099:1121	salivary protein output following sucrose (ρ = 0.72, p = 0.02) and changes in levels of proline-rich protein and mucin 7 following capsaicin (ρ = 0.71, p = 0.02, ρ = 0.68, p = 0.04, respectively)	1099:1293	FPD did correlate with changes in salivary protein output following sucrose (ρ = 0.72, p = 0.02) and changes in levels of proline-rich protein and mucin 7 following capsaicin (ρ = 0.71, p = 0.02, ρ = 0.68, p = 0.04, respectively).
31278319	6	1	theme	intensity	749:757	arg1	perception					759:768	intensity perception	749:768	intensity perception of sucrose, caffeine, menthol and capsaicin solutions	749:822	FPD, diameter and position were measured and participants rated intensity perception of sucrose, caffeine, menthol and capsaicin solutions.
31278319	1	2	theme	taste	164:168	arg1	buds					170:173	Fungiform papillae house taste buds	139:173	Fungiform papillae house taste buds on the anterior dorsal tongue	139:203	Fungiform papillae house taste buds on the anterior dorsal tongue.
31278319	6	3	theme	caffeine	782:789	arg1	solutions					814:822	sucrose, caffeine, menthol and capsaicin solutions	773:822	sucrose, caffeine, menthol and capsaicin solutions	773:822	FPD, diameter and position were measured and participants rated intensity perception of sucrose, caffeine, menthol and capsaicin solutions.
31278319	11	4	theme	taste	1517:1521	arg1	intensity					1504:1512	the perceived intensity	1490:1512	the perceived intensity of taste	1490:1521	These results suggest that over six months fungiform papillae are anatomically stable, playing a greater role in mediating the physiological salivary response to stimuli rather than determining the perceived intensity of taste.
31278319	0	5	theme	salivary	99:106	arg1	response					108:115	salivary response	99:115	salivary response	99:115	Anatomical stability of human fungiform papillae and relationship with oral perception measured by salivary response and intensity rating.
31278319	1	6	from	buds	170:173	arg1	tongue					198:203	the anterior dorsal tongue	178:203	the anterior dorsal tongue	178:203	Fungiform papillae house taste buds on the anterior dorsal tongue.
31278319	3	7	theme	saliva	372:377	arg1	composition					357:367	composition	357:367	composition	357:367	Gustatory reflexes modulate the amount and composition of saliva subsequently produced, and thus may be a more physiologically objective measure of tastant-receptor interactions.
31278319	3	7	theme	saliva	372:377	arg1	amount					346:351	amount	346:351	amount	346:351	Gustatory reflexes modulate the amount and composition of saliva subsequently produced, and thus may be a more physiologically objective measure of tastant-receptor interactions.
31278319	3	7	theme	saliva	372:377	arg1	saliva					372:377	saliva	372:377	saliva	372:377	Gustatory reflexes modulate the amount and composition of saliva subsequently produced, and thus may be a more physiologically objective measure of tastant-receptor interactions.
31278319	7	8	theme	protein	891:897	arg1	composition					899:909	protein composition	891:909	protein composition	891:909	Salivary flow rate, protein concentration and relative changes in protein composition were measured following each tastant.
31278319	10	9	theme	mucin	1212:1216	arg1	levels					1177:1182	levels	1177:1182	levels of proline-rich protein and mucin 7	1177:1218	FPD did correlate with changes in salivary protein output following sucrose (ρ = 0.72, p = 0.02) and changes in levels of proline-rich protein and mucin 7 following capsaicin (ρ = 0.71, p = 0.02, ρ = 0.68, p = 0.04, respectively).
31278319	4	10	theme	Taste	493:497	arg1	perception					499:508	Taste perception	493:508	Taste perception	493:508	Taste perception fluctuates with time but the stability of individual fungiform papillae is unclear.
31278319	10	11	from	changes	1166:1172	arg1	levels					1177:1182	levels	1177:1182	levels of proline-rich protein and mucin 7	1177:1218	FPD did correlate with changes in salivary protein output following sucrose (ρ = 0.72, p = 0.02) and changes in levels of proline-rich protein and mucin 7 following capsaicin (ρ = 0.71, p = 0.02, ρ = 0.68, p = 0.04, respectively).
31278319	2	12	theme	papillae	290:297	arg1	density					299:305	fungiform papillae density	280:305	fungiform papillae density (FPD)	280:311	Literature is inconclusive as to whether taste perception correlates with fungiform papillae density (FPD).
31278319	2	12	theme	papillae	290:297	arg1	FPD					308:310	FPD	308:310	FPD	308:310	Literature is inconclusive as to whether taste perception correlates with fungiform papillae density (FPD).
31278319	3	13	theme	objective	441:449	arg1	measure					451:457	a more physiologically objective measure	418:457	a more physiologically objective measure of tastant-receptor interactions	418:490	Gustatory reflexes modulate the amount and composition of saliva subsequently produced, and thus may be a more physiologically objective measure of tastant-receptor interactions.
31278319	2	14	theme	fungiform	280:288	arg1	density					299:305	fungiform papillae density	280:305	fungiform papillae density (FPD)	280:311	Literature is inconclusive as to whether taste perception correlates with fungiform papillae density (FPD).
31278319	2	14	theme	fungiform	280:288	arg1	FPD					308:310	FPD	308:310	FPD	308:310	Literature is inconclusive as to whether taste perception correlates with fungiform papillae density (FPD).
31278319	11	15	theme	physiological	1423:1435	arg1	response					1446:1453	the physiological salivary response	1419:1453	the physiological salivary response to stimuli	1419:1464	These results suggest that over six months fungiform papillae are anatomically stable, playing a greater role in mediating the physiological salivary response to stimuli rather than determining the perceived intensity of taste.
31278319	10	16	dep	sucrose	1133:1139	arg1	p = 0.02					1152:1159	p = 0.02	1152:1159	p = 0.02	1152:1159	FPD did correlate with changes in salivary protein output following sucrose (ρ = 0.72, p = 0.02) and changes in levels of proline-rich protein and mucin 7 following capsaicin (ρ = 0.71, p = 0.02, ρ = 0.68, p = 0.04, respectively).
31278319	7	17	from	rate	839:842	arg1	composition					899:909	protein composition	891:909	protein composition	891:909	Salivary flow rate, protein concentration and relative changes in protein composition were measured following each tastant.
31278319	6	18	theme	solutions	814:822	arg1	perception					759:768	intensity perception	749:768	intensity perception of sucrose, caffeine, menthol and capsaicin solutions	749:822	FPD, diameter and position were measured and participants rated intensity perception of sucrose, caffeine, menthol and capsaicin solutions.
31278319	11	19	theme	salivary	1437:1444	arg1	response					1446:1453	the physiological salivary response	1419:1453	the physiological salivary response to stimuli	1419:1464	These results suggest that over six months fungiform papillae are anatomically stable, playing a greater role in mediating the physiological salivary response to stimuli rather than determining the perceived intensity of taste.
31278319	1	20	theme	anterior	182:189	arg1	tongue					198:203	the anterior dorsal tongue	178:203	the anterior dorsal tongue	178:203	Fungiform papillae house taste buds on the anterior dorsal tongue.
31278319	0	21	theme	Anatomical	0:9	arg1	stability					11:19	Anatomical stability	0:19	Anatomical stability of human fungiform papillae and relationship with oral perception	0:85	Anatomical stability of human fungiform papillae and relationship with oral perception measured by salivary response and intensity rating.
31278319	6	22	theme	capsaicin	804:812	arg1	solutions					814:822	sucrose, caffeine, menthol and capsaicin solutions	773:822	sucrose, caffeine, menthol and capsaicin solutions	773:822	FPD, diameter and position were measured and participants rated intensity perception of sucrose, caffeine, menthol and capsaicin solutions.
31278319	1	23	theme	dorsal	191:196	arg1	tongue					198:203	the anterior dorsal tongue	178:203	the anterior dorsal tongue	178:203	Fungiform papillae house taste buds on the anterior dorsal tongue.
31278319	0	24	theme	human	24:28	arg1	fungiform					30:38	human fungiform papillae and relationship	24:64	fungiform	30:38	Anatomical stability of human fungiform papillae and relationship with oral perception measured by salivary response and intensity rating.
31278319	5	25	theme	healthy	618:624	arg1	volunteers					626:635	ten healthy volunteers	614:635	ten healthy volunteers	614:635	This study followed ten healthy volunteers longitudinally at baseline, one and six months.
31278319	7	26	theme	relative	871:878	arg1	changes					880:886	relative changes	871:886	relative changes in protein composition	871:909	Salivary flow rate, protein concentration and relative changes in protein composition were measured following each tastant.
31278319	3	27	theme	interactions	479:490	arg1	measure					451:457	a more physiologically objective measure	418:457	a more physiologically objective measure of tastant-receptor interactions	418:490	Gustatory reflexes modulate the amount and composition of saliva subsequently produced, and thus may be a more physiologically objective measure of tastant-receptor interactions.
31278319	7	28	theme	Salivary	825:832	arg1	rate					839:842	Salivary flow rate	825:842	Salivary flow rate	825:842	Salivary flow rate, protein concentration and relative changes in protein composition were measured following each tastant.
31278319	0	29	theme	intensity	121:129	arg1	rating					131:136	intensity rating	121:136	intensity rating	121:136	Anatomical stability of human fungiform papillae and relationship with oral perception measured by salivary response and intensity rating.
31278319	7	30	theme	protein	845:851	arg1	concentration					853:865	protein concentration	845:865	protein concentration	845:865	Salivary flow rate, protein concentration and relative changes in protein composition were measured following each tastant.
31278319	7	31	theme	flow	834:837	arg1	rate					839:842	Salivary flow rate	825:842	Salivary flow rate	825:842	Salivary flow rate, protein concentration and relative changes in protein composition were measured following each tastant.
31278319	0	32	theme	fungiform	30:38	arg1	stability					11:19	Anatomical stability	0:19	Anatomical stability of human fungiform papillae and relationship with oral perception	0:85	Anatomical stability of human fungiform papillae and relationship with oral perception measured by salivary response and intensity rating.
31278319	4	33	theme	individual	552:561	arg1	papillae					573:580	individual fungiform papillae	552:580	individual fungiform papillae	552:580	Taste perception fluctuates with time but the stability of individual fungiform papillae is unclear.
31278319	3	34	dep	amount	346:351	arg1	the					342:344	the	342:344	the	342:344	Gustatory reflexes modulate the amount and composition of saliva subsequently produced, and thus may be a more physiologically objective measure of tastant-receptor interactions.
31278319	10	35	dep	capsaicin	1230:1238	arg1	ρ = 0.71					1241:1248	ρ = 0.71	1241:1248	ρ = 0.71	1241:1248	FPD did correlate with changes in salivary protein output following sucrose (ρ = 0.72, p = 0.02) and changes in levels of proline-rich protein and mucin 7 following capsaicin (ρ = 0.71, p = 0.02, ρ = 0.68, p = 0.04, respectively).
31278319	7	36	from	concentration	853:865	arg1	composition					899:909	protein composition	891:909	protein composition	891:909	Salivary flow rate, protein concentration and relative changes in protein composition were measured following each tastant.
31278319	6	37	theme	menthol	792:798	arg1	solutions					814:822	sucrose, caffeine, menthol and capsaicin solutions	773:822	sucrose, caffeine, menthol and capsaicin solutions	773:822	FPD, diameter and position were measured and participants rated intensity perception of sucrose, caffeine, menthol and capsaicin solutions.
31278319	3	38	theme	tastant-receptor	462:477	arg1	interactions					479:490	tastant-receptor interactions	462:490	tastant-receptor interactions	462:490	Gustatory reflexes modulate the amount and composition of saliva subsequently produced, and thus may be a more physiologically objective measure of tastant-receptor interactions.
31278319	0	39	theme	relationship	53:64	arg1	stability					11:19	Anatomical stability	0:19	Anatomical stability of human fungiform papillae and relationship with oral perception	0:85	Anatomical stability of human fungiform papillae and relationship with oral perception measured by salivary response and intensity rating.
31278319	10	40	from	sucrose	1133:1139	arg1	levels					1177:1182	levels	1177:1182	levels of proline-rich protein and mucin 7	1177:1218	FPD did correlate with changes in salivary protein output following sucrose (ρ = 0.72, p = 0.02) and changes in levels of proline-rich protein and mucin 7 following capsaicin (ρ = 0.71, p = 0.02, ρ = 0.68, p = 0.04, respectively).
31278319	4	41	theme	papillae	573:580	arg1	unclear					585:591	unclear	585:591	unclear	585:591	Taste perception fluctuates with time but the stability of individual fungiform papillae is unclear.
31278319	4	41	theme	papillae	573:580	arg1	stability					539:547	the stability	535:547	the stability of individual fungiform papillae	535:580	Taste perception fluctuates with time but the stability of individual fungiform papillae is unclear.
31278319	3	42	theme	Gustatory	314:322	arg1	reflexes					324:331	Gustatory reflexes	314:331	Gustatory reflexes	314:331	Gustatory reflexes modulate the amount and composition of saliva subsequently produced, and thus may be a more physiologically objective measure of tastant-receptor interactions.
31278319	11	43	theme	fungiform	1339:1347	arg1	papillae					1349:1356	fungiform papillae	1339:1356	fungiform papillae	1339:1356	These results suggest that over six months fungiform papillae are anatomically stable, playing a greater role in mediating the physiological salivary response to stimuli rather than determining the perceived intensity of taste.
31278319	0	44	dep	fungiform	30:38	arg1	papillae					40:47	papillae	40:47	papillae	40:47	Anatomical stability of human fungiform papillae and relationship with oral perception measured by salivary response and intensity rating.
31278319	0	45	with	stability	11:19	arg1	perception					76:85	oral perception	71:85	oral perception	71:85	Anatomical stability of human fungiform papillae and relationship with oral perception measured by salivary response and intensity rating.
31278319	4	46	theme	fungiform	563:571	arg1	papillae					573:580	individual fungiform papillae	552:580	individual fungiform papillae	552:580	Taste perception fluctuates with time but the stability of individual fungiform papillae is unclear.
31278319	7	47	from	changes	880:886	arg1	composition					899:909	protein composition	891:909	protein composition	891:909	Salivary flow rate, protein concentration and relative changes in protein composition were measured following each tastant.
31278319	10	48	from	changes	1088:1094	arg1	output					1116:1121	salivary protein output	1099:1121	salivary protein output following sucrose (ρ = 0.72, p = 0.02) and changes in levels of proline-rich protein and mucin 7 following capsaicin (ρ = 0.71, p = 0.02, ρ = 0.68, p = 0.04, respectively)	1099:1293	FPD did correlate with changes in salivary protein output following sucrose (ρ = 0.72, p = 0.02) and changes in levels of proline-rich protein and mucin 7 following capsaicin (ρ = 0.71, p = 0.02, ρ = 0.68, p = 0.04, respectively).
31278319	6	49	theme	sucrose	773:779	arg1	solutions					814:822	sucrose, caffeine, menthol and capsaicin solutions	773:822	sucrose, caffeine, menthol and capsaicin solutions	773:822	FPD, diameter and position were measured and participants rated intensity perception of sucrose, caffeine, menthol and capsaicin solutions.
31278319	1	50	theme	Fungiform	139:147	arg1	buds					170:173	Fungiform papillae house taste buds	139:173	Fungiform papillae house taste buds on the anterior dorsal tongue	139:203	Fungiform papillae house taste buds on the anterior dorsal tongue.
31278319	10	51	theme	protein	1200:1206	arg1	levels					1177:1182	levels	1177:1182	levels of proline-rich protein and mucin 7	1177:1218	FPD did correlate with changes in salivary protein output following sucrose (ρ = 0.72, p = 0.02) and changes in levels of proline-rich protein and mucin 7 following capsaicin (ρ = 0.71, p = 0.02, ρ = 0.68, p = 0.04, respectively).
31278319	0	52	theme	oral	71:74	arg1	perception					76:85	oral perception	71:85	oral perception	71:85	Anatomical stability of human fungiform papillae and relationship with oral perception measured by salivary response and intensity rating.
31278319	8	53	from	months	998:1003	arg1	unchanged					981:989	unchanged	981:989	unchanged	981:989	FPD, diameter and position were unchanged at six months.
31278319	1	54	theme	papillae	149:156	arg1	buds					170:173	Fungiform papillae house taste buds	139:173	Fungiform papillae house taste buds on the anterior dorsal tongue	139:203	Fungiform papillae house taste buds on the anterior dorsal tongue.
31278319	11	55	theme	perceived	1494:1502	arg1	intensity					1504:1512	the perceived intensity	1490:1512	the perceived intensity of taste	1490:1521	These results suggest that over six months fungiform papillae are anatomically stable, playing a greater role in mediating the physiological salivary response to stimuli rather than determining the perceived intensity of taste.
31278319	10	56	theme	proline-rich	1187:1198	arg1	protein					1200:1206	proline-rich protein	1187:1206	proline-rich protein	1187:1206	FPD did correlate with changes in salivary protein output following sucrose (ρ = 0.72, p = 0.02) and changes in levels of proline-rich protein and mucin 7 following capsaicin (ρ = 0.71, p = 0.02, ρ = 0.68, p = 0.04, respectively).
31278319	11	57	theme	greater	1393:1399	arg1	role					1401:1404	a greater role	1391:1404	a greater role	1391:1404	These results suggest that over six months fungiform papillae are anatomically stable, playing a greater role in mediating the physiological salivary response to stimuli rather than determining the perceived intensity of taste.
31278319	10	58	theme	protein	1108:1114	arg1	output					1116:1121	salivary protein output	1099:1121	salivary protein output following sucrose (ρ = 0.72, p = 0.02) and changes in levels of proline-rich protein and mucin 7 following capsaicin (ρ = 0.71, p = 0.02, ρ = 0.68, p = 0.04, respectively)	1099:1293	FPD did correlate with changes in salivary protein output following sucrose (ρ = 0.72, p = 0.02) and changes in levels of proline-rich protein and mucin 7 following capsaicin (ρ = 0.71, p = 0.02, ρ = 0.68, p = 0.04, respectively).
31278319	2	59	theme	taste	247:251	arg1	perception					253:262	taste perception	247:262	taste perception	247:262	Literature is inconclusive as to whether taste perception correlates with fungiform papillae density (FPD).
31278319	1	60	theme	house	158:162	arg1	buds					170:173	Fungiform papillae house taste buds	139:173	Fungiform papillae house taste buds on the anterior dorsal tongue	139:203	Fungiform papillae house taste buds on the anterior dorsal tongue.
30296052	2	0	theme	potassium	673:681	arg1	network					725:731	the second network	714:731	the second network	714:731	To address these limitations, we developed a novel κ-carrageenan/polyacrylamide (KC/PAM) DN hydrogel through a dual physical-cross-linking strategy, with the ductile, hydrophobically associated PAM being the first network, and the rigid potassium ion (K+) cross-linked KC being the second network.
30296052	2	0	theme	potassium	673:681	arg1	KC					705:706	the rigid potassium ion (K+) cross-linked KC	663:706	the rigid potassium ion (K+) cross-linked KC	663:706	To address these limitations, we developed a novel κ-carrageenan/polyacrylamide (KC/PAM) DN hydrogel through a dual physical-cross-linking strategy, with the ductile, hydrophobically associated PAM being the first network, and the rigid potassium ion (K+) cross-linked KC being the second network.
30296052	4	1	theme	unique	1074:1079	arg1	structures					1109:1118	their unique dual physical-cross-linking structures	1068:1118	their unique dual physical-cross-linking structures	1068:1118	Moreover, because of their unique dual physical-cross-linking structures, the KC/PAM hydrogels also demonstrated rapid self-recovery, remarkable notch-insensitivity, self-healing capability, as well as excellent cytocompatibility toward stem cells.
30296052	3	2	theme	dual	738:741	arg1	hydrogels					779:787	The dual physically cross-linked DN (DPC-DN) hydrogels	734:787	The dual physically cross-linked DN (DPC-DN) hydrogels with optimized KC concentration	734:819	The dual physically cross-linked DN (DPC-DN) hydrogels with optimized KC concentration exhibit excellent fracture tensile stress (1320 ± 46 kPa) and toughness (fracture energy: 6900 ± 280 kJ/m3), comparable to those fully chemically linked DN hydrogels and physically chemically cross-linked hybrid DN hydrogels.
30296052	4	3	theme	physical-cross-linking	1086:1107	arg1	structures					1109:1118	their unique dual physical-cross-linking structures	1068:1118	their unique dual physical-cross-linking structures	1068:1118	Moreover, because of their unique dual physical-cross-linking structures, the KC/PAM hydrogels also demonstrated rapid self-recovery, remarkable notch-insensitivity, self-healing capability, as well as excellent cytocompatibility toward stem cells.
30296052	2	4	link	cross-linked	692:703	arg1	network					725:731	the second network	714:731	the second network	714:731	To address these limitations, we developed a novel κ-carrageenan/polyacrylamide (KC/PAM) DN hydrogel through a dual physical-cross-linking strategy, with the ductile, hydrophobically associated PAM being the first network, and the rigid potassium ion (K+) cross-linked KC being the second network.
30296052	2	4	link	cross-linked	692:703	arg1	KC					705:706	the rigid potassium ion (K+) cross-linked KC	663:706	the rigid potassium ion (K+) cross-linked KC	663:706	To address these limitations, we developed a novel κ-carrageenan/polyacrylamide (KC/PAM) DN hydrogel through a dual physical-cross-linking strategy, with the ductile, hydrophobically associated PAM being the first network, and the rigid potassium ion (K+) cross-linked KC being the second network.
30296052	1	5	theme	mechanical	211:220	arg1	attributes					222:231	extraordinary mechanical attributes	197:231	extraordinary mechanical attributes	197:231	Chemically linked double network (DN) hydrogels display extraordinary mechanical attributes but mostly suffer from poor self-healing property and unsatisfactory biocompatibility due to the irreversible breaks in their chemical-linked networks and the use of toxic chemical cross-linking agents.
30296052	3	6	theme	KC	804:805	arg1	concentration					807:819	optimized KC concentration	794:819	optimized KC concentration	794:819	The dual physically cross-linked DN (DPC-DN) hydrogels with optimized KC concentration exhibit excellent fracture tensile stress (1320 ± 46 kPa) and toughness (fracture energy: 6900 ± 280 kJ/m3), comparable to those fully chemically linked DN hydrogels and physically chemically cross-linked hybrid DN hydrogels.
30296052	1	7	from	breaks	343:348	arg1	networks					375:382	their chemical-linked networks	353:382	their chemical-linked networks	353:382	Chemically linked double network (DN) hydrogels display extraordinary mechanical attributes but mostly suffer from poor self-healing property and unsatisfactory biocompatibility due to the irreversible breaks in their chemical-linked networks and the use of toxic chemical cross-linking agents.
30296052	1	7	from	breaks	343:348	arg1	use					392:394	the use	388:394	the use of toxic chemical cross-linking agents	388:433	Chemically linked double network (DN) hydrogels display extraordinary mechanical attributes but mostly suffer from poor self-healing property and unsatisfactory biocompatibility due to the irreversible breaks in their chemical-linked networks and the use of toxic chemical cross-linking agents.
30296052	4	8	theme	rapid	1160:1164	arg1	self-recovery					1166:1178	rapid self-recovery	1160:1178	rapid self-recovery	1160:1178	Moreover, because of their unique dual physical-cross-linking structures, the KC/PAM hydrogels also demonstrated rapid self-recovery, remarkable notch-insensitivity, self-healing capability, as well as excellent cytocompatibility toward stem cells.
30296052	4	8	theme	rapid	1160:1164	arg1	notch-insensitivity					1192:1210	remarkable notch-insensitivity	1181:1210	remarkable notch-insensitivity	1181:1210	Moreover, because of their unique dual physical-cross-linking structures, the KC/PAM hydrogels also demonstrated rapid self-recovery, remarkable notch-insensitivity, self-healing capability, as well as excellent cytocompatibility toward stem cells.
30296052	4	8	theme	rapid	1160:1164	arg1	capability					1226:1235	self-healing capability	1213:1235	self-healing capability	1213:1235	Moreover, because of their unique dual physical-cross-linking structures, the KC/PAM hydrogels also demonstrated rapid self-recovery, remarkable notch-insensitivity, self-healing capability, as well as excellent cytocompatibility toward stem cells.
30296052	3	9	theme	fracture	839:846	arg1	kPa					874:876	1320 ± 46 kPa	864:876	1320 ± 46 kPa	864:876	The dual physically cross-linked DN (DPC-DN) hydrogels with optimized KC concentration exhibit excellent fracture tensile stress (1320 ± 46 kPa) and toughness (fracture energy: 6900 ± 280 kJ/m3), comparable to those fully chemically linked DN hydrogels and physically chemically cross-linked hybrid DN hydrogels.
30296052	3	9	theme	fracture	839:846	arg1	stress					856:861	excellent fracture tensile stress	829:861	excellent fracture tensile stress (1320 ± 46 kPa)	829:877	The dual physically cross-linked DN (DPC-DN) hydrogels with optimized KC concentration exhibit excellent fracture tensile stress (1320 ± 46 kPa) and toughness (fracture energy: 6900 ± 280 kJ/m3), comparable to those fully chemically linked DN hydrogels and physically chemically cross-linked hybrid DN hydrogels.
30296052	3	9	theme	fracture	839:846	arg1	kJ/m3					922:926	fracture energy: 6900 ± 280 kJ/m3	894:926	fracture energy: 6900 ± 280 kJ/m3	894:926	The dual physically cross-linked DN (DPC-DN) hydrogels with optimized KC concentration exhibit excellent fracture tensile stress (1320 ± 46 kPa) and toughness (fracture energy: 6900 ± 280 kJ/m3), comparable to those fully chemically linked DN hydrogels and physically chemically cross-linked hybrid DN hydrogels.
30296052	3	9	theme	fracture	839:846	arg1	hydrogels					1036:1044	comparable to those fully chemically linked DN hydrogels and physically chemically cross-linked hybrid DN hydrogels	930:1044	comparable to those fully chemically linked DN hydrogels and physically chemically cross-linked hybrid DN hydrogels	930:1044	The dual physically cross-linked DN (DPC-DN) hydrogels with optimized KC concentration exhibit excellent fracture tensile stress (1320 ± 46 kPa) and toughness (fracture energy: 6900 ± 280 kJ/m3), comparable to those fully chemically linked DN hydrogels and physically chemically cross-linked hybrid DN hydrogels.
30296052	5	10	with	hydrogels	1384:1392	arg1	biocompatibility					1436:1451	biocompatibility	1436:1451	biocompatibility	1436:1451	Accordingly, this work presents a new strategy toward fabricating self-repairing DPC-DN hydrogels with outstanding mechanical behaviors and biocompatibility.
30296052	5	10	with	hydrogels	1384:1392	arg1	behaviors					1422:1430	outstanding mechanical behaviors	1399:1430	outstanding mechanical behaviors	1399:1430	Accordingly, this work presents a new strategy toward fabricating self-repairing DPC-DN hydrogels with outstanding mechanical behaviors and biocompatibility.
30296052	1	11	theme	poor	256:259	arg1	property					274:281	poor self-healing property	256:281	poor self-healing property	256:281	Chemically linked double network (DN) hydrogels display extraordinary mechanical attributes but mostly suffer from poor self-healing property and unsatisfactory biocompatibility due to the irreversible breaks in their chemical-linked networks and the use of toxic chemical cross-linking agents.
30296052	1	12	theme	double	159:164	arg1	hydrogels					179:187	Chemically linked double network (DN) hydrogels	141:187	Chemically linked double network (DN) hydrogels	141:187	Chemically linked double network (DN) hydrogels display extraordinary mechanical attributes but mostly suffer from poor self-healing property and unsatisfactory biocompatibility due to the irreversible breaks in their chemical-linked networks and the use of toxic chemical cross-linking agents.
30296052	3	13	theme	fracture	894:901	arg1	kJ/m3					922:926	fracture energy: 6900 ± 280 kJ/m3	894:926	fracture energy: 6900 ± 280 kJ/m3	894:926	The dual physically cross-linked DN (DPC-DN) hydrogels with optimized KC concentration exhibit excellent fracture tensile stress (1320 ± 46 kPa) and toughness (fracture energy: 6900 ± 280 kJ/m3), comparable to those fully chemically linked DN hydrogels and physically chemically cross-linked hybrid DN hydrogels.
30296052	3	13	theme	fracture	894:901	arg1	stress					856:861	excellent fracture tensile stress	829:861	excellent fracture tensile stress (1320 ± 46 kPa)	829:877	The dual physically cross-linked DN (DPC-DN) hydrogels with optimized KC concentration exhibit excellent fracture tensile stress (1320 ± 46 kPa) and toughness (fracture energy: 6900 ± 280 kJ/m3), comparable to those fully chemically linked DN hydrogels and physically chemically cross-linked hybrid DN hydrogels.
30296052	2	14	theme	first	644:648	arg1	PAM					630:632	the ductile, hydrophobically associated PAM	590:632	the ductile, hydrophobically associated PAM	590:632	To address these limitations, we developed a novel κ-carrageenan/polyacrylamide (KC/PAM) DN hydrogel through a dual physical-cross-linking strategy, with the ductile, hydrophobically associated PAM being the first network, and the rigid potassium ion (K+) cross-linked KC being the second network.
30296052	2	14	theme	first	644:648	arg1	network					650:656	the first network	640:656	the first network	640:656	To address these limitations, we developed a novel κ-carrageenan/polyacrylamide (KC/PAM) DN hydrogel through a dual physical-cross-linking strategy, with the ductile, hydrophobically associated PAM being the first network, and the rigid potassium ion (K+) cross-linked KC being the second network.
30296052	4	15	theme	excellent	1249:1257	arg1	cytocompatibility					1259:1275	excellent cytocompatibility	1249:1275	rapid self-recovery, remarkable notch-insensitivity, self-healing capability, as well as excellent cytocompatibility toward stem cells	1160:1293	Moreover, because of their unique dual physical-cross-linking structures, the KC/PAM hydrogels also demonstrated rapid self-recovery, remarkable notch-insensitivity, self-healing capability, as well as excellent cytocompatibility toward stem cells.
30296052	4	16	theme	self-healing	1213:1224	arg1	self-recovery					1166:1178	rapid self-recovery	1160:1178	rapid self-recovery	1160:1178	Moreover, because of their unique dual physical-cross-linking structures, the KC/PAM hydrogels also demonstrated rapid self-recovery, remarkable notch-insensitivity, self-healing capability, as well as excellent cytocompatibility toward stem cells.
30296052	4	16	theme	self-healing	1213:1224	arg1	capability					1226:1235	self-healing capability	1213:1235	self-healing capability	1213:1235	Moreover, because of their unique dual physical-cross-linking structures, the KC/PAM hydrogels also demonstrated rapid self-recovery, remarkable notch-insensitivity, self-healing capability, as well as excellent cytocompatibility toward stem cells.
30296052	5	17	theme	DPC-DN	1377:1382	arg1	hydrogels					1384:1392	self-repairing DPC-DN hydrogels	1362:1392	self-repairing DPC-DN hydrogels with outstanding mechanical behaviors and biocompatibility	1362:1451	Accordingly, this work presents a new strategy toward fabricating self-repairing DPC-DN hydrogels with outstanding mechanical behaviors and biocompatibility.
30296052	0	18	theme	Superior	79:86	arg1	Performance					101:111	Superior Self-Healing Performance	79:111	Superior Self-Healing Performance for Biomedical Application	79:138	Dual Physically Cross-Linked κ-Carrageenan-Based Double Network Hydrogels with Superior Self-Healing Performance for Biomedical Application.
30296052	3	19	theme	±	916:916	arg1	kJ/m3					922:926	fracture energy: 6900 ± 280 kJ/m3	894:926	fracture energy: 6900 ± 280 kJ/m3	894:926	The dual physically cross-linked DN (DPC-DN) hydrogels with optimized KC concentration exhibit excellent fracture tensile stress (1320 ± 46 kPa) and toughness (fracture energy: 6900 ± 280 kJ/m3), comparable to those fully chemically linked DN hydrogels and physically chemically cross-linked hybrid DN hydrogels.
30296052	3	19	theme	±	916:916	arg1	stress					856:861	excellent fracture tensile stress	829:861	excellent fracture tensile stress (1320 ± 46 kPa)	829:877	The dual physically cross-linked DN (DPC-DN) hydrogels with optimized KC concentration exhibit excellent fracture tensile stress (1320 ± 46 kPa) and toughness (fracture energy: 6900 ± 280 kJ/m3), comparable to those fully chemically linked DN hydrogels and physically chemically cross-linked hybrid DN hydrogels.
30296052	2	20	theme	physical-cross-linking	552:573	arg1	strategy					575:582	a novel κ-carrageenan/polyacrylamide (KC/PAM) DN hydrogel through a dual physical-cross-linking strategy	479:582	a novel κ-carrageenan/polyacrylamide (KC/PAM) DN hydrogel through a dual physical-cross-linking strategy	479:582	To address these limitations, we developed a novel κ-carrageenan/polyacrylamide (KC/PAM) DN hydrogel through a dual physical-cross-linking strategy, with the ductile, hydrophobically associated PAM being the first network, and the rigid potassium ion (K+) cross-linked KC being the second network.
30296052	6	21	theme	DN	1470:1471	arg1	hydrogels					1473:1481	DN hydrogels	1470:1481	DN hydrogels	1470:1481	The new type of DN hydrogels demonstrates strong potentiality in many challenging biomedical applications such as artificial diaphragm, tendon, and cartilage.
30296052	1	22	theme	toxic	399:403	arg1	agents					428:433	toxic chemical cross-linking agents	399:433	toxic chemical cross-linking agents	399:433	Chemically linked double network (DN) hydrogels display extraordinary mechanical attributes but mostly suffer from poor self-healing property and unsatisfactory biocompatibility due to the irreversible breaks in their chemical-linked networks and the use of toxic chemical cross-linking agents.
30296052	0	23	theme	Biomedical	117:126	arg1	Application					128:138	Biomedical Application	117:138	Biomedical Application	117:138	Dual Physically Cross-Linked κ-Carrageenan-Based Double Network Hydrogels with Superior Self-Healing Performance for Biomedical Application.
30296052	1	24	theme	cross-linking	414:426	arg1	agents					428:433	toxic chemical cross-linking agents	399:433	toxic chemical cross-linking agents	399:433	Chemically linked double network (DN) hydrogels display extraordinary mechanical attributes but mostly suffer from poor self-healing property and unsatisfactory biocompatibility due to the irreversible breaks in their chemical-linked networks and the use of toxic chemical cross-linking agents.
30296052	2	25	theme	cross-linked	692:703	arg1	network					725:731	the second network	714:731	the second network	714:731	To address these limitations, we developed a novel κ-carrageenan/polyacrylamide (KC/PAM) DN hydrogel through a dual physical-cross-linking strategy, with the ductile, hydrophobically associated PAM being the first network, and the rigid potassium ion (K+) cross-linked KC being the second network.
30296052	2	25	theme	cross-linked	692:703	arg1	KC					705:706	the rigid potassium ion (K+) cross-linked KC	663:706	the rigid potassium ion (K+) cross-linked KC	663:706	To address these limitations, we developed a novel κ-carrageenan/polyacrylamide (KC/PAM) DN hydrogel through a dual physical-cross-linking strategy, with the ductile, hydrophobically associated PAM being the first network, and the rigid potassium ion (K+) cross-linked KC being the second network.
30296052	0	26	theme	Network	56:62	arg1	Hydrogels					64:72	κ-Carrageenan-Based Double Network Hydrogels	29:72	κ-Carrageenan-Based Double Network Hydrogels	29:72	Dual Physically Cross-Linked κ-Carrageenan-Based Double Network Hydrogels with Superior Self-Healing Performance for Biomedical Application.
30296052	0	27	theme	Self-Healing	88:99	arg1	Performance					101:111	Superior Self-Healing Performance	79:111	Superior Self-Healing Performance for Biomedical Application	79:138	Dual Physically Cross-Linked κ-Carrageenan-Based Double Network Hydrogels with Superior Self-Healing Performance for Biomedical Application.
30296052	1	28	theme	irreversible	330:341	arg1	breaks					343:348	the irreversible breaks	326:348	the irreversible breaks in their chemical-linked networks and the use of toxic chemical cross-linking agents	326:433	Chemically linked double network (DN) hydrogels display extraordinary mechanical attributes but mostly suffer from poor self-healing property and unsatisfactory biocompatibility due to the irreversible breaks in their chemical-linked networks and the use of toxic chemical cross-linking agents.
30296052	2	29	theme	second	718:723	arg1	network					725:731	the second network	714:731	the second network	714:731	To address these limitations, we developed a novel κ-carrageenan/polyacrylamide (KC/PAM) DN hydrogel through a dual physical-cross-linking strategy, with the ductile, hydrophobically associated PAM being the first network, and the rigid potassium ion (K+) cross-linked KC being the second network.
30296052	2	29	theme	second	718:723	arg1	KC					705:706	the rigid potassium ion (K+) cross-linked KC	663:706	the rigid potassium ion (K+) cross-linked KC	663:706	To address these limitations, we developed a novel κ-carrageenan/polyacrylamide (KC/PAM) DN hydrogel through a dual physical-cross-linking strategy, with the ductile, hydrophobically associated PAM being the first network, and the rigid potassium ion (K+) cross-linked KC being the second network.
30296052	2	30	theme	novel	481:485	arg1	strategy					575:582	a novel κ-carrageenan/polyacrylamide (KC/PAM) DN hydrogel through a dual physical-cross-linking strategy	479:582	a novel κ-carrageenan/polyacrylamide (KC/PAM) DN hydrogel through a dual physical-cross-linking strategy	479:582	To address these limitations, we developed a novel κ-carrageenan/polyacrylamide (KC/PAM) DN hydrogel through a dual physical-cross-linking strategy, with the ductile, hydrophobically associated PAM being the first network, and the rigid potassium ion (K+) cross-linked KC being the second network.
30296052	6	31	theme	biomedical	1536:1545	arg1	applications					1547:1558	many challenging biomedical applications	1519:1558	many challenging biomedical applications such as artificial diaphragm, tendon, and cartilage	1519:1610	The new type of DN hydrogels demonstrates strong potentiality in many challenging biomedical applications such as artificial diaphragm, tendon, and cartilage.
30296052	6	31	theme	biomedical	1536:1545	arg1	tendon					1590:1595	tendon	1590:1595	tendon	1590:1595	The new type of DN hydrogels demonstrates strong potentiality in many challenging biomedical applications such as artificial diaphragm, tendon, and cartilage.
30296052	6	31	theme	biomedical	1536:1545	arg1	cartilage					1602:1610	cartilage	1602:1610	cartilage	1602:1610	The new type of DN hydrogels demonstrates strong potentiality in many challenging biomedical applications such as artificial diaphragm, tendon, and cartilage.
30296052	6	31	theme	biomedical	1536:1545	arg1	diaphragm					1579:1587	artificial diaphragm	1568:1587	artificial diaphragm	1568:1587	The new type of DN hydrogels demonstrates strong potentiality in many challenging biomedical applications such as artificial diaphragm, tendon, and cartilage.
30296052	5	32	theme	mechanical	1411:1420	arg1	behaviors					1422:1430	outstanding mechanical behaviors	1399:1430	outstanding mechanical behaviors	1399:1430	Accordingly, this work presents a new strategy toward fabricating self-repairing DPC-DN hydrogels with outstanding mechanical behaviors and biocompatibility.
30296052	6	33	theme	many	1519:1522	arg1	applications					1547:1558	many challenging biomedical applications	1519:1558	many challenging biomedical applications such as artificial diaphragm, tendon, and cartilage	1519:1610	The new type of DN hydrogels demonstrates strong potentiality in many challenging biomedical applications such as artificial diaphragm, tendon, and cartilage.
30296052	6	33	theme	many	1519:1522	arg1	tendon					1590:1595	tendon	1590:1595	tendon	1590:1595	The new type of DN hydrogels demonstrates strong potentiality in many challenging biomedical applications such as artificial diaphragm, tendon, and cartilage.
30296052	6	33	theme	many	1519:1522	arg1	cartilage					1602:1610	cartilage	1602:1610	cartilage	1602:1610	The new type of DN hydrogels demonstrates strong potentiality in many challenging biomedical applications such as artificial diaphragm, tendon, and cartilage.
30296052	6	33	theme	many	1519:1522	arg1	diaphragm					1579:1587	artificial diaphragm	1568:1587	artificial diaphragm	1568:1587	The new type of DN hydrogels demonstrates strong potentiality in many challenging biomedical applications such as artificial diaphragm, tendon, and cartilage.
30296052	1	34	theme	chemical-linked	359:373	arg1	networks					375:382	their chemical-linked networks	353:382	their chemical-linked networks	353:382	Chemically linked double network (DN) hydrogels display extraordinary mechanical attributes but mostly suffer from poor self-healing property and unsatisfactory biocompatibility due to the irreversible breaks in their chemical-linked networks and the use of toxic chemical cross-linking agents.
30296052	0	35	theme	Double	49:54	arg1	Hydrogels					64:72	κ-Carrageenan-Based Double Network Hydrogels	29:72	κ-Carrageenan-Based Double Network Hydrogels	29:72	Dual Physically Cross-Linked κ-Carrageenan-Based Double Network Hydrogels with Superior Self-Healing Performance for Biomedical Application.
30296052	2	36	theme	hydrogel	528:535	arg1	strategy					575:582	a novel κ-carrageenan/polyacrylamide (KC/PAM) DN hydrogel through a dual physical-cross-linking strategy	479:582	a novel κ-carrageenan/polyacrylamide (KC/PAM) DN hydrogel through a dual physical-cross-linking strategy	479:582	To address these limitations, we developed a novel κ-carrageenan/polyacrylamide (KC/PAM) DN hydrogel through a dual physical-cross-linking strategy, with the ductile, hydrophobically associated PAM being the first network, and the rigid potassium ion (K+) cross-linked KC being the second network.
30296052	2	37	dep	ductile	594:600	arg1	associated					619:628	associated	619:628	associated	619:628	To address these limitations, we developed a novel κ-carrageenan/polyacrylamide (KC/PAM) DN hydrogel through a dual physical-cross-linking strategy, with the ductile, hydrophobically associated PAM being the first network, and the rigid potassium ion (K+) cross-linked KC being the second network.
30296052	3	38	theme	linked	967:972	arg1	hydrogels					977:985	those fully chemically linked DN hydrogels	944:985	those fully chemically linked DN hydrogels	944:985	The dual physically cross-linked DN (DPC-DN) hydrogels with optimized KC concentration exhibit excellent fracture tensile stress (1320 ± 46 kPa) and toughness (fracture energy: 6900 ± 280 kJ/m3), comparable to those fully chemically linked DN hydrogels and physically chemically cross-linked hybrid DN hydrogels.
30296052	1	39	link	linked	152:157	arg1	hydrogels					179:187	Chemically linked double network (DN) hydrogels	141:187	Chemically linked double network (DN) hydrogels	141:187	Chemically linked double network (DN) hydrogels display extraordinary mechanical attributes but mostly suffer from poor self-healing property and unsatisfactory biocompatibility due to the irreversible breaks in their chemical-linked networks and the use of toxic chemical cross-linking agents.
30296052	1	40	theme	self-healing	261:272	arg1	property					274:281	poor self-healing property	256:281	poor self-healing property	256:281	Chemically linked double network (DN) hydrogels display extraordinary mechanical attributes but mostly suffer from poor self-healing property and unsatisfactory biocompatibility due to the irreversible breaks in their chemical-linked networks and the use of toxic chemical cross-linking agents.
30296052	3	41	theme	cross-linked	1013:1024	arg1	stress					856:861	excellent fracture tensile stress	829:861	excellent fracture tensile stress (1320 ± 46 kPa)	829:877	The dual physically cross-linked DN (DPC-DN) hydrogels with optimized KC concentration exhibit excellent fracture tensile stress (1320 ± 46 kPa) and toughness (fracture energy: 6900 ± 280 kJ/m3), comparable to those fully chemically linked DN hydrogels and physically chemically cross-linked hybrid DN hydrogels.
30296052	3	41	theme	cross-linked	1013:1024	arg1	hydrogels					1036:1044	comparable to those fully chemically linked DN hydrogels and physically chemically cross-linked hybrid DN hydrogels	930:1044	comparable to those fully chemically linked DN hydrogels and physically chemically cross-linked hybrid DN hydrogels	930:1044	The dual physically cross-linked DN (DPC-DN) hydrogels with optimized KC concentration exhibit excellent fracture tensile stress (1320 ± 46 kPa) and toughness (fracture energy: 6900 ± 280 kJ/m3), comparable to those fully chemically linked DN hydrogels and physically chemically cross-linked hybrid DN hydrogels.
30296052	3	42	with	hydrogels	779:787	arg1	concentration					807:819	optimized KC concentration	794:819	optimized KC concentration	794:819	The dual physically cross-linked DN (DPC-DN) hydrogels with optimized KC concentration exhibit excellent fracture tensile stress (1320 ± 46 kPa) and toughness (fracture energy: 6900 ± 280 kJ/m3), comparable to those fully chemically linked DN hydrogels and physically chemically cross-linked hybrid DN hydrogels.
30296052	4	43	theme	dual	1081:1084	arg1	structures					1109:1118	their unique dual physical-cross-linking structures	1068:1118	their unique dual physical-cross-linking structures	1068:1118	Moreover, because of their unique dual physical-cross-linking structures, the KC/PAM hydrogels also demonstrated rapid self-recovery, remarkable notch-insensitivity, self-healing capability, as well as excellent cytocompatibility toward stem cells.
30296052	3	44	theme	DN	767:768	arg1	hydrogels					779:787	The dual physically cross-linked DN (DPC-DN) hydrogels	734:787	The dual physically cross-linked DN (DPC-DN) hydrogels with optimized KC concentration	734:819	The dual physically cross-linked DN (DPC-DN) hydrogels with optimized KC concentration exhibit excellent fracture tensile stress (1320 ± 46 kPa) and toughness (fracture energy: 6900 ± 280 kJ/m3), comparable to those fully chemically linked DN hydrogels and physically chemically cross-linked hybrid DN hydrogels.
30296052	2	45	theme	rigid	667:671	arg1	network					725:731	the second network	714:731	the second network	714:731	To address these limitations, we developed a novel κ-carrageenan/polyacrylamide (KC/PAM) DN hydrogel through a dual physical-cross-linking strategy, with the ductile, hydrophobically associated PAM being the first network, and the rigid potassium ion (K+) cross-linked KC being the second network.
30296052	2	45	theme	rigid	667:671	arg1	KC					705:706	the rigid potassium ion (K+) cross-linked KC	663:706	the rigid potassium ion (K+) cross-linked KC	663:706	To address these limitations, we developed a novel κ-carrageenan/polyacrylamide (KC/PAM) DN hydrogel through a dual physical-cross-linking strategy, with the ductile, hydrophobically associated PAM being the first network, and the rigid potassium ion (K+) cross-linked KC being the second network.
30296052	6	46	theme	artificial	1568:1577	arg1	diaphragm					1579:1587	artificial diaphragm	1568:1587	artificial diaphragm	1568:1587	The new type of DN hydrogels demonstrates strong potentiality in many challenging biomedical applications such as artificial diaphragm, tendon, and cartilage.
30296052	3	47	theme	DN	1033:1034	arg1	stress					856:861	excellent fracture tensile stress	829:861	excellent fracture tensile stress (1320 ± 46 kPa)	829:877	The dual physically cross-linked DN (DPC-DN) hydrogels with optimized KC concentration exhibit excellent fracture tensile stress (1320 ± 46 kPa) and toughness (fracture energy: 6900 ± 280 kJ/m3), comparable to those fully chemically linked DN hydrogels and physically chemically cross-linked hybrid DN hydrogels.
30296052	3	47	theme	DN	1033:1034	arg1	hydrogels					1036:1044	comparable to those fully chemically linked DN hydrogels and physically chemically cross-linked hybrid DN hydrogels	930:1044	comparable to those fully chemically linked DN hydrogels and physically chemically cross-linked hybrid DN hydrogels	930:1044	The dual physically cross-linked DN (DPC-DN) hydrogels with optimized KC concentration exhibit excellent fracture tensile stress (1320 ± 46 kPa) and toughness (fracture energy: 6900 ± 280 kJ/m3), comparable to those fully chemically linked DN hydrogels and physically chemically cross-linked hybrid DN hydrogels.
30296052	4	48	theme	remarkable	1181:1190	arg1	self-recovery					1166:1178	rapid self-recovery	1160:1178	rapid self-recovery	1160:1178	Moreover, because of their unique dual physical-cross-linking structures, the KC/PAM hydrogels also demonstrated rapid self-recovery, remarkable notch-insensitivity, self-healing capability, as well as excellent cytocompatibility toward stem cells.
30296052	4	48	theme	remarkable	1181:1190	arg1	notch-insensitivity					1192:1210	remarkable notch-insensitivity	1181:1210	remarkable notch-insensitivity	1181:1210	Moreover, because of their unique dual physical-cross-linking structures, the KC/PAM hydrogels also demonstrated rapid self-recovery, remarkable notch-insensitivity, self-healing capability, as well as excellent cytocompatibility toward stem cells.
30296052	1	49	theme	extraordinary	197:209	arg1	attributes					222:231	extraordinary mechanical attributes	197:231	extraordinary mechanical attributes	197:231	Chemically linked double network (DN) hydrogels display extraordinary mechanical attributes but mostly suffer from poor self-healing property and unsatisfactory biocompatibility due to the irreversible breaks in their chemical-linked networks and the use of toxic chemical cross-linking agents.
30296052	2	50	theme	K+	688:689	arg1	network					725:731	the second network	714:731	the second network	714:731	To address these limitations, we developed a novel κ-carrageenan/polyacrylamide (KC/PAM) DN hydrogel through a dual physical-cross-linking strategy, with the ductile, hydrophobically associated PAM being the first network, and the rigid potassium ion (K+) cross-linked KC being the second network.
30296052	2	50	theme	K+	688:689	arg1	KC					705:706	the rigid potassium ion (K+) cross-linked KC	663:706	the rigid potassium ion (K+) cross-linked KC	663:706	To address these limitations, we developed a novel κ-carrageenan/polyacrylamide (KC/PAM) DN hydrogel through a dual physical-cross-linking strategy, with the ductile, hydrophobically associated PAM being the first network, and the rigid potassium ion (K+) cross-linked KC being the second network.
30296052	3	51	theme	DPC-DN	771:776	arg1	hydrogels					779:787	The dual physically cross-linked DN (DPC-DN) hydrogels	734:787	The dual physically cross-linked DN (DPC-DN) hydrogels with optimized KC concentration	734:819	The dual physically cross-linked DN (DPC-DN) hydrogels with optimized KC concentration exhibit excellent fracture tensile stress (1320 ± 46 kPa) and toughness (fracture energy: 6900 ± 280 kJ/m3), comparable to those fully chemically linked DN hydrogels and physically chemically cross-linked hybrid DN hydrogels.
30296052	2	52	theme	ion	683:685	arg1	network					725:731	the second network	714:731	the second network	714:731	To address these limitations, we developed a novel κ-carrageenan/polyacrylamide (KC/PAM) DN hydrogel through a dual physical-cross-linking strategy, with the ductile, hydrophobically associated PAM being the first network, and the rigid potassium ion (K+) cross-linked KC being the second network.
30296052	2	52	theme	ion	683:685	arg1	KC					705:706	the rigid potassium ion (K+) cross-linked KC	663:706	the rigid potassium ion (K+) cross-linked KC	663:706	To address these limitations, we developed a novel κ-carrageenan/polyacrylamide (KC/PAM) DN hydrogel through a dual physical-cross-linking strategy, with the ductile, hydrophobically associated PAM being the first network, and the rigid potassium ion (K+) cross-linked KC being the second network.
30296052	3	53	link	cross-linked	754:765	arg1	hydrogels					779:787	The dual physically cross-linked DN (DPC-DN) hydrogels	734:787	The dual physically cross-linked DN (DPC-DN) hydrogels with optimized KC concentration	734:819	The dual physically cross-linked DN (DPC-DN) hydrogels with optimized KC concentration exhibit excellent fracture tensile stress (1320 ± 46 kPa) and toughness (fracture energy: 6900 ± 280 kJ/m3), comparable to those fully chemically linked DN hydrogels and physically chemically cross-linked hybrid DN hydrogels.
30296052	3	54	theme	optimized	794:802	arg1	concentration					807:819	optimized KC concentration	794:819	optimized KC concentration	794:819	The dual physically cross-linked DN (DPC-DN) hydrogels with optimized KC concentration exhibit excellent fracture tensile stress (1320 ± 46 kPa) and toughness (fracture energy: 6900 ± 280 kJ/m3), comparable to those fully chemically linked DN hydrogels and physically chemically cross-linked hybrid DN hydrogels.
30296052	3	55	theme	tensile	848:854	arg1	kPa					874:876	1320 ± 46 kPa	864:876	1320 ± 46 kPa	864:876	The dual physically cross-linked DN (DPC-DN) hydrogels with optimized KC concentration exhibit excellent fracture tensile stress (1320 ± 46 kPa) and toughness (fracture energy: 6900 ± 280 kJ/m3), comparable to those fully chemically linked DN hydrogels and physically chemically cross-linked hybrid DN hydrogels.
30296052	3	55	theme	tensile	848:854	arg1	stress					856:861	excellent fracture tensile stress	829:861	excellent fracture tensile stress (1320 ± 46 kPa)	829:877	The dual physically cross-linked DN (DPC-DN) hydrogels with optimized KC concentration exhibit excellent fracture tensile stress (1320 ± 46 kPa) and toughness (fracture energy: 6900 ± 280 kJ/m3), comparable to those fully chemically linked DN hydrogels and physically chemically cross-linked hybrid DN hydrogels.
30296052	3	55	theme	tensile	848:854	arg1	kJ/m3					922:926	fracture energy: 6900 ± 280 kJ/m3	894:926	fracture energy: 6900 ± 280 kJ/m3	894:926	The dual physically cross-linked DN (DPC-DN) hydrogels with optimized KC concentration exhibit excellent fracture tensile stress (1320 ± 46 kPa) and toughness (fracture energy: 6900 ± 280 kJ/m3), comparable to those fully chemically linked DN hydrogels and physically chemically cross-linked hybrid DN hydrogels.
30296052	3	55	theme	tensile	848:854	arg1	hydrogels					1036:1044	comparable to those fully chemically linked DN hydrogels and physically chemically cross-linked hybrid DN hydrogels	930:1044	comparable to those fully chemically linked DN hydrogels and physically chemically cross-linked hybrid DN hydrogels	930:1044	The dual physically cross-linked DN (DPC-DN) hydrogels with optimized KC concentration exhibit excellent fracture tensile stress (1320 ± 46 kPa) and toughness (fracture energy: 6900 ± 280 kJ/m3), comparable to those fully chemically linked DN hydrogels and physically chemically cross-linked hybrid DN hydrogels.
30296052	1	56	theme	linked	152:157	arg1	hydrogels					179:187	Chemically linked double network (DN) hydrogels	141:187	Chemically linked double network (DN) hydrogels	141:187	Chemically linked double network (DN) hydrogels display extraordinary mechanical attributes but mostly suffer from poor self-healing property and unsatisfactory biocompatibility due to the irreversible breaks in their chemical-linked networks and the use of toxic chemical cross-linking agents.
30296052	3	57	theme	excellent	829:837	arg1	kPa					874:876	1320 ± 46 kPa	864:876	1320 ± 46 kPa	864:876	The dual physically cross-linked DN (DPC-DN) hydrogels with optimized KC concentration exhibit excellent fracture tensile stress (1320 ± 46 kPa) and toughness (fracture energy: 6900 ± 280 kJ/m3), comparable to those fully chemically linked DN hydrogels and physically chemically cross-linked hybrid DN hydrogels.
30296052	3	57	theme	excellent	829:837	arg1	stress					856:861	excellent fracture tensile stress	829:861	excellent fracture tensile stress (1320 ± 46 kPa)	829:877	The dual physically cross-linked DN (DPC-DN) hydrogels with optimized KC concentration exhibit excellent fracture tensile stress (1320 ± 46 kPa) and toughness (fracture energy: 6900 ± 280 kJ/m3), comparable to those fully chemically linked DN hydrogels and physically chemically cross-linked hybrid DN hydrogels.
30296052	3	57	theme	excellent	829:837	arg1	kJ/m3					922:926	fracture energy: 6900 ± 280 kJ/m3	894:926	fracture energy: 6900 ± 280 kJ/m3	894:926	The dual physically cross-linked DN (DPC-DN) hydrogels with optimized KC concentration exhibit excellent fracture tensile stress (1320 ± 46 kPa) and toughness (fracture energy: 6900 ± 280 kJ/m3), comparable to those fully chemically linked DN hydrogels and physically chemically cross-linked hybrid DN hydrogels.
30296052	3	57	theme	excellent	829:837	arg1	hydrogels					1036:1044	comparable to those fully chemically linked DN hydrogels and physically chemically cross-linked hybrid DN hydrogels	930:1044	comparable to those fully chemically linked DN hydrogels and physically chemically cross-linked hybrid DN hydrogels	930:1044	The dual physically cross-linked DN (DPC-DN) hydrogels with optimized KC concentration exhibit excellent fracture tensile stress (1320 ± 46 kPa) and toughness (fracture energy: 6900 ± 280 kJ/m3), comparable to those fully chemically linked DN hydrogels and physically chemically cross-linked hybrid DN hydrogels.
30296052	6	58	from	potentiality	1503:1514	arg1	applications					1547:1558	many challenging biomedical applications	1519:1558	many challenging biomedical applications such as artificial diaphragm, tendon, and cartilage	1519:1610	The new type of DN hydrogels demonstrates strong potentiality in many challenging biomedical applications such as artificial diaphragm, tendon, and cartilage.
30296052	6	58	from	potentiality	1503:1514	arg1	tendon					1590:1595	tendon	1590:1595	tendon	1590:1595	The new type of DN hydrogels demonstrates strong potentiality in many challenging biomedical applications such as artificial diaphragm, tendon, and cartilage.
30296052	6	58	from	potentiality	1503:1514	arg1	cartilage					1602:1610	cartilage	1602:1610	cartilage	1602:1610	The new type of DN hydrogels demonstrates strong potentiality in many challenging biomedical applications such as artificial diaphragm, tendon, and cartilage.
30296052	6	58	from	potentiality	1503:1514	arg1	diaphragm					1579:1587	artificial diaphragm	1568:1587	artificial diaphragm	1568:1587	The new type of DN hydrogels demonstrates strong potentiality in many challenging biomedical applications such as artificial diaphragm, tendon, and cartilage.
30296052	1	59	theme	network	166:172	arg1	hydrogels					179:187	Chemically linked double network (DN) hydrogels	141:187	Chemically linked double network (DN) hydrogels	141:187	Chemically linked double network (DN) hydrogels display extraordinary mechanical attributes but mostly suffer from poor self-healing property and unsatisfactory biocompatibility due to the irreversible breaks in their chemical-linked networks and the use of toxic chemical cross-linking agents.
30296052	3	60	theme	energy	903:908	arg1	kJ/m3					922:926	fracture energy: 6900 ± 280 kJ/m3	894:926	fracture energy: 6900 ± 280 kJ/m3	894:926	The dual physically cross-linked DN (DPC-DN) hydrogels with optimized KC concentration exhibit excellent fracture tensile stress (1320 ± 46 kPa) and toughness (fracture energy: 6900 ± 280 kJ/m3), comparable to those fully chemically linked DN hydrogels and physically chemically cross-linked hybrid DN hydrogels.
30296052	3	60	theme	energy	903:908	arg1	stress					856:861	excellent fracture tensile stress	829:861	excellent fracture tensile stress (1320 ± 46 kPa)	829:877	The dual physically cross-linked DN (DPC-DN) hydrogels with optimized KC concentration exhibit excellent fracture tensile stress (1320 ± 46 kPa) and toughness (fracture energy: 6900 ± 280 kJ/m3), comparable to those fully chemically linked DN hydrogels and physically chemically cross-linked hybrid DN hydrogels.
30296052	5	61	theme	self-repairing	1362:1375	arg1	hydrogels					1384:1392	self-repairing DPC-DN hydrogels	1362:1392	self-repairing DPC-DN hydrogels with outstanding mechanical behaviors and biocompatibility	1362:1451	Accordingly, this work presents a new strategy toward fabricating self-repairing DPC-DN hydrogels with outstanding mechanical behaviors and biocompatibility.
30296052	3	62	theme	±	869:869	arg1	kPa					874:876	1320 ± 46 kPa	864:876	1320 ± 46 kPa	864:876	The dual physically cross-linked DN (DPC-DN) hydrogels with optimized KC concentration exhibit excellent fracture tensile stress (1320 ± 46 kPa) and toughness (fracture energy: 6900 ± 280 kJ/m3), comparable to those fully chemically linked DN hydrogels and physically chemically cross-linked hybrid DN hydrogels.
30296052	3	62	theme	±	869:869	arg1	stress					856:861	excellent fracture tensile stress	829:861	excellent fracture tensile stress (1320 ± 46 kPa)	829:877	The dual physically cross-linked DN (DPC-DN) hydrogels with optimized KC concentration exhibit excellent fracture tensile stress (1320 ± 46 kPa) and toughness (fracture energy: 6900 ± 280 kJ/m3), comparable to those fully chemically linked DN hydrogels and physically chemically cross-linked hybrid DN hydrogels.
30296052	6	63	theme	strong	1496:1501	arg1	potentiality					1503:1514	strong potentiality	1496:1514	strong potentiality in many challenging biomedical applications such as artificial diaphragm, tendon, and cartilage	1496:1610	The new type of DN hydrogels demonstrates strong potentiality in many challenging biomedical applications such as artificial diaphragm, tendon, and cartilage.
30296052	1	64	theme	DN	175:176	arg1	hydrogels					179:187	Chemically linked double network (DN) hydrogels	141:187	Chemically linked double network (DN) hydrogels	141:187	Chemically linked double network (DN) hydrogels display extraordinary mechanical attributes but mostly suffer from poor self-healing property and unsatisfactory biocompatibility due to the irreversible breaks in their chemical-linked networks and the use of toxic chemical cross-linking agents.
30296052	6	65	theme	hydrogels	1473:1481	arg1	type					1462:1465	The new type	1454:1465	The new type of DN hydrogels	1454:1481	The new type of DN hydrogels demonstrates strong potentiality in many challenging biomedical applications such as artificial diaphragm, tendon, and cartilage.
30296052	2	66	theme	dual	547:550	arg1	strategy					575:582	a novel κ-carrageenan/polyacrylamide (KC/PAM) DN hydrogel through a dual physical-cross-linking strategy	479:582	a novel κ-carrageenan/polyacrylamide (KC/PAM) DN hydrogel through a dual physical-cross-linking strategy	479:582	To address these limitations, we developed a novel κ-carrageenan/polyacrylamide (KC/PAM) DN hydrogel through a dual physical-cross-linking strategy, with the ductile, hydrophobically associated PAM being the first network, and the rigid potassium ion (K+) cross-linked KC being the second network.
30296052	5	67	theme	new	1330:1332	arg1	strategy					1334:1341	a new strategy	1328:1341	a new strategy toward fabricating self-repairing DPC-DN hydrogels with outstanding mechanical behaviors and biocompatibility	1328:1451	Accordingly, this work presents a new strategy toward fabricating self-repairing DPC-DN hydrogels with outstanding mechanical behaviors and biocompatibility.
30296052	1	68	theme	chemical	405:412	arg1	agents					428:433	toxic chemical cross-linking agents	399:433	toxic chemical cross-linking agents	399:433	Chemically linked double network (DN) hydrogels display extraordinary mechanical attributes but mostly suffer from poor self-healing property and unsatisfactory biocompatibility due to the irreversible breaks in their chemical-linked networks and the use of toxic chemical cross-linking agents.
30296052	2	69	theme	ductile	594:600	arg1	PAM					630:632	the ductile, hydrophobically associated PAM	590:632	the ductile, hydrophobically associated PAM	590:632	To address these limitations, we developed a novel κ-carrageenan/polyacrylamide (KC/PAM) DN hydrogel through a dual physical-cross-linking strategy, with the ductile, hydrophobically associated PAM being the first network, and the rigid potassium ion (K+) cross-linked KC being the second network.
30296052	2	69	theme	ductile	594:600	arg1	network					650:656	the first network	640:656	the first network	640:656	To address these limitations, we developed a novel κ-carrageenan/polyacrylamide (KC/PAM) DN hydrogel through a dual physical-cross-linking strategy, with the ductile, hydrophobically associated PAM being the first network, and the rigid potassium ion (K+) cross-linked KC being the second network.
30296052	6	70	theme	new	1458:1460	arg1	type					1462:1465	The new type	1454:1465	The new type of DN hydrogels	1454:1481	The new type of DN hydrogels demonstrates strong potentiality in many challenging biomedical applications such as artificial diaphragm, tendon, and cartilage.
30296052	1	71	theme	agents	428:433	arg1	networks					375:382	their chemical-linked networks	353:382	their chemical-linked networks	353:382	Chemically linked double network (DN) hydrogels display extraordinary mechanical attributes but mostly suffer from poor self-healing property and unsatisfactory biocompatibility due to the irreversible breaks in their chemical-linked networks and the use of toxic chemical cross-linking agents.
30296052	1	71	theme	agents	428:433	arg1	use					392:394	the use	388:394	the use of toxic chemical cross-linking agents	388:433	Chemically linked double network (DN) hydrogels display extraordinary mechanical attributes but mostly suffer from poor self-healing property and unsatisfactory biocompatibility due to the irreversible breaks in their chemical-linked networks and the use of toxic chemical cross-linking agents.
30296052	3	72	theme	comparable	930:939	arg1	stress					856:861	excellent fracture tensile stress	829:861	excellent fracture tensile stress (1320 ± 46 kPa)	829:877	The dual physically cross-linked DN (DPC-DN) hydrogels with optimized KC concentration exhibit excellent fracture tensile stress (1320 ± 46 kPa) and toughness (fracture energy: 6900 ± 280 kJ/m3), comparable to those fully chemically linked DN hydrogels and physically chemically cross-linked hybrid DN hydrogels.
30296052	3	72	theme	comparable	930:939	arg1	hydrogels					1036:1044	comparable to those fully chemically linked DN hydrogels and physically chemically cross-linked hybrid DN hydrogels	930:1044	comparable to those fully chemically linked DN hydrogels and physically chemically cross-linked hybrid DN hydrogels	930:1044	The dual physically cross-linked DN (DPC-DN) hydrogels with optimized KC concentration exhibit excellent fracture tensile stress (1320 ± 46 kPa) and toughness (fracture energy: 6900 ± 280 kJ/m3), comparable to those fully chemically linked DN hydrogels and physically chemically cross-linked hybrid DN hydrogels.
30296052	4	73	theme	stem	1284:1287	arg1	cells					1289:1293	stem cells	1284:1293	stem cells	1284:1293	Moreover, because of their unique dual physical-cross-linking structures, the KC/PAM hydrogels also demonstrated rapid self-recovery, remarkable notch-insensitivity, self-healing capability, as well as excellent cytocompatibility toward stem cells.
30296052	2	74	theme	KC/PAM	517:522	arg1	strategy					575:582	a novel κ-carrageenan/polyacrylamide (KC/PAM) DN hydrogel through a dual physical-cross-linking strategy	479:582	a novel κ-carrageenan/polyacrylamide (KC/PAM) DN hydrogel through a dual physical-cross-linking strategy	479:582	To address these limitations, we developed a novel κ-carrageenan/polyacrylamide (KC/PAM) DN hydrogel through a dual physical-cross-linking strategy, with the ductile, hydrophobically associated PAM being the first network, and the rigid potassium ion (K+) cross-linked KC being the second network.
30296052	0	75	theme	κ-Carrageenan-Based	29:47	arg1	Hydrogels					64:72	κ-Carrageenan-Based Double Network Hydrogels	29:72	κ-Carrageenan-Based Double Network Hydrogels	29:72	Dual Physically Cross-Linked κ-Carrageenan-Based Double Network Hydrogels with Superior Self-Healing Performance for Biomedical Application.
30296052	2	76	theme	κ-carrageenan/polyacrylamide	487:514	arg1	strategy					575:582	a novel κ-carrageenan/polyacrylamide (KC/PAM) DN hydrogel through a dual physical-cross-linking strategy	479:582	a novel κ-carrageenan/polyacrylamide (KC/PAM) DN hydrogel through a dual physical-cross-linking strategy	479:582	To address these limitations, we developed a novel κ-carrageenan/polyacrylamide (KC/PAM) DN hydrogel through a dual physical-cross-linking strategy, with the ductile, hydrophobically associated PAM being the first network, and the rigid potassium ion (K+) cross-linked KC being the second network.
30296052	3	77	link	cross-linked	1013:1024	arg1	stress					856:861	excellent fracture tensile stress	829:861	excellent fracture tensile stress (1320 ± 46 kPa)	829:877	The dual physically cross-linked DN (DPC-DN) hydrogels with optimized KC concentration exhibit excellent fracture tensile stress (1320 ± 46 kPa) and toughness (fracture energy: 6900 ± 280 kJ/m3), comparable to those fully chemically linked DN hydrogels and physically chemically cross-linked hybrid DN hydrogels.
30296052	3	77	link	cross-linked	1013:1024	arg1	hydrogels					1036:1044	comparable to those fully chemically linked DN hydrogels and physically chemically cross-linked hybrid DN hydrogels	930:1044	comparable to those fully chemically linked DN hydrogels and physically chemically cross-linked hybrid DN hydrogels	930:1044	The dual physically cross-linked DN (DPC-DN) hydrogels with optimized KC concentration exhibit excellent fracture tensile stress (1320 ± 46 kPa) and toughness (fracture energy: 6900 ± 280 kJ/m3), comparable to those fully chemically linked DN hydrogels and physically chemically cross-linked hybrid DN hydrogels.
30296052	5	78	theme	outstanding	1399:1409	arg1	behaviors					1422:1430	outstanding mechanical behaviors	1399:1430	outstanding mechanical behaviors	1399:1430	Accordingly, this work presents a new strategy toward fabricating self-repairing DPC-DN hydrogels with outstanding mechanical behaviors and biocompatibility.
30296052	6	79	theme	challenging	1524:1534	arg1	applications					1547:1558	many challenging biomedical applications	1519:1558	many challenging biomedical applications such as artificial diaphragm, tendon, and cartilage	1519:1610	The new type of DN hydrogels demonstrates strong potentiality in many challenging biomedical applications such as artificial diaphragm, tendon, and cartilage.
30296052	6	79	theme	challenging	1524:1534	arg1	tendon					1590:1595	tendon	1590:1595	tendon	1590:1595	The new type of DN hydrogels demonstrates strong potentiality in many challenging biomedical applications such as artificial diaphragm, tendon, and cartilage.
30296052	6	79	theme	challenging	1524:1534	arg1	cartilage					1602:1610	cartilage	1602:1610	cartilage	1602:1610	The new type of DN hydrogels demonstrates strong potentiality in many challenging biomedical applications such as artificial diaphragm, tendon, and cartilage.
30296052	6	79	theme	challenging	1524:1534	arg1	diaphragm					1579:1587	artificial diaphragm	1568:1587	artificial diaphragm	1568:1587	The new type of DN hydrogels demonstrates strong potentiality in many challenging biomedical applications such as artificial diaphragm, tendon, and cartilage.
30296052	2	80	theme	through	537:543	arg1	strategy					575:582	a novel κ-carrageenan/polyacrylamide (KC/PAM) DN hydrogel through a dual physical-cross-linking strategy	479:582	a novel κ-carrageenan/polyacrylamide (KC/PAM) DN hydrogel through a dual physical-cross-linking strategy	479:582	To address these limitations, we developed a novel κ-carrageenan/polyacrylamide (KC/PAM) DN hydrogel through a dual physical-cross-linking strategy, with the ductile, hydrophobically associated PAM being the first network, and the rigid potassium ion (K+) cross-linked KC being the second network.
30296052	3	81	link	linked	967:972	arg1	hydrogels					977:985	those fully chemically linked DN hydrogels	944:985	those fully chemically linked DN hydrogels	944:985	The dual physically cross-linked DN (DPC-DN) hydrogels with optimized KC concentration exhibit excellent fracture tensile stress (1320 ± 46 kPa) and toughness (fracture energy: 6900 ± 280 kJ/m3), comparable to those fully chemically linked DN hydrogels and physically chemically cross-linked hybrid DN hydrogels.
30296052	2	82	theme	DN	525:526	arg1	strategy					575:582	a novel κ-carrageenan/polyacrylamide (KC/PAM) DN hydrogel through a dual physical-cross-linking strategy	479:582	a novel κ-carrageenan/polyacrylamide (KC/PAM) DN hydrogel through a dual physical-cross-linking strategy	479:582	To address these limitations, we developed a novel κ-carrageenan/polyacrylamide (KC/PAM) DN hydrogel through a dual physical-cross-linking strategy, with the ductile, hydrophobically associated PAM being the first network, and the rigid potassium ion (K+) cross-linked KC being the second network.
30296052	1	83	link	chemical-linked	359:373	arg1	networks					375:382	their chemical-linked networks	353:382	their chemical-linked networks	353:382	Chemically linked double network (DN) hydrogels display extraordinary mechanical attributes but mostly suffer from poor self-healing property and unsatisfactory biocompatibility due to the irreversible breaks in their chemical-linked networks and the use of toxic chemical cross-linking agents.
30296052	3	84	theme	DN	974:975	arg1	hydrogels					977:985	those fully chemically linked DN hydrogels	944:985	those fully chemically linked DN hydrogels	944:985	The dual physically cross-linked DN (DPC-DN) hydrogels with optimized KC concentration exhibit excellent fracture tensile stress (1320 ± 46 kPa) and toughness (fracture energy: 6900 ± 280 kJ/m3), comparable to those fully chemically linked DN hydrogels and physically chemically cross-linked hybrid DN hydrogels.
30296052	3	85	theme	hybrid	1026:1031	arg1	stress					856:861	excellent fracture tensile stress	829:861	excellent fracture tensile stress (1320 ± 46 kPa)	829:877	The dual physically cross-linked DN (DPC-DN) hydrogels with optimized KC concentration exhibit excellent fracture tensile stress (1320 ± 46 kPa) and toughness (fracture energy: 6900 ± 280 kJ/m3), comparable to those fully chemically linked DN hydrogels and physically chemically cross-linked hybrid DN hydrogels.
30296052	3	85	theme	hybrid	1026:1031	arg1	hydrogels					1036:1044	comparable to those fully chemically linked DN hydrogels and physically chemically cross-linked hybrid DN hydrogels	930:1044	comparable to those fully chemically linked DN hydrogels and physically chemically cross-linked hybrid DN hydrogels	930:1044	The dual physically cross-linked DN (DPC-DN) hydrogels with optimized KC concentration exhibit excellent fracture tensile stress (1320 ± 46 kPa) and toughness (fracture energy: 6900 ± 280 kJ/m3), comparable to those fully chemically linked DN hydrogels and physically chemically cross-linked hybrid DN hydrogels.
30296052	4	86	theme	KC/PAM	1125:1130	arg1	hydrogels					1132:1140	the KC/PAM hydrogels	1121:1140	the KC/PAM hydrogels	1121:1140	Moreover, because of their unique dual physical-cross-linking structures, the KC/PAM hydrogels also demonstrated rapid self-recovery, remarkable notch-insensitivity, self-healing capability, as well as excellent cytocompatibility toward stem cells.
30296052	3	87	theme	cross-linked	754:765	arg1	hydrogels					779:787	The dual physically cross-linked DN (DPC-DN) hydrogels	734:787	The dual physically cross-linked DN (DPC-DN) hydrogels with optimized KC concentration	734:819	The dual physically cross-linked DN (DPC-DN) hydrogels with optimized KC concentration exhibit excellent fracture tensile stress (1320 ± 46 kPa) and toughness (fracture energy: 6900 ± 280 kJ/m3), comparable to those fully chemically linked DN hydrogels and physically chemically cross-linked hybrid DN hydrogels.
30296052	1	88	theme	unsatisfactory	287:300	arg1	biocompatibility					302:317	unsatisfactory biocompatibility	287:317	unsatisfactory biocompatibility	287:317	Chemically linked double network (DN) hydrogels display extraordinary mechanical attributes but mostly suffer from poor self-healing property and unsatisfactory biocompatibility due to the irreversible breaks in their chemical-linked networks and the use of toxic chemical cross-linking agents.
31396162	6	0	theme	ERK1/2	1004:1009	arg1	phosphorylation					1011:1025	ERK1/2 phosphorylation	1004:1025	ERK1/2 phosphorylation	1004:1025	Intracellular cAMP production, Ca2+ increase and β-arrestin 2 recruitment were evaluated by BRET, CREB, and ERK1/2 phosphorylation by Western blotting.
31396162	7	1	theme	12-h	1048:1051	arg1	expression					1058:1067	12-h gene expression	1048:1067	12-h gene expression	1048:1067	12-h gene expression, and 8- and 24-h progesterone and estradiol synthesis were measured by real-time PCR and immunoassay, respectively.
31396162	9	2	theme	p	1718:1718	arg1	<					1720:1720	p < 0.05	1718:1725	p < 0.05	1718:1725	Kinetics analysis revealed that intracellular Ca2+ increased upon cell treatment by 4 μg/ml Gonal-f®, while equal concentrations of biosimilars failed to induced a response (Kruskal-Wallis test; p < 0.05; n = 3).
31396162	4	3	theme	preparation	580:590	arg1	specific-patterns					592:608	preparation specific-patterns	580:608	preparation specific-patterns of glycan species (Kruskal-Wallis test; p < 0.05, n = 6) and by glycotope mapping	580:690	Gonadotropin N-glycosylation profiles were analyzed by ELISA lectin assay, revealing preparation specific-patterns of glycan species (Kruskal-Wallis test; p < 0.05, n = 6) and by glycotope mapping.
31396162	2	4	theme	dependent	287:295	arg1	profile					279:285	a unique glycosylation profile	256:285	a unique glycosylation profile dependent on source cells	256:311	They have specific FSH activity and a unique glycosylation profile dependent on source cells.
31396162	2	5	theme	glycosylation	265:277	arg1	profile					279:285	a unique glycosylation profile	256:285	a unique glycosylation profile dependent on source cells	256:311	They have specific FSH activity and a unique glycosylation profile dependent on source cells.
31396162	5	6	theme	granulosa	807:815	arg1	hGLC					831:834	hGLC	831:834	hGLC	831:834	Increasing concentrations of Gonal-f® or biosimilar (1 × 10-3-1 × 103 ng/ml) were used for treating human primary granulosa lutein cells (hGLC) and FSH receptor (FSHR)-transfected HEK293 cells in vitro.
31396162	5	6	theme	granulosa	807:815	arg1	cells					824:828	human primary granulosa lutein cells	793:828	human primary granulosa lutein cells (hGLC)	793:835	Increasing concentrations of Gonal-f® or biosimilar (1 × 10-3-1 × 103 ng/ml) were used for treating human primary granulosa lutein cells (hGLC) and FSH receptor (FSHR)-transfected HEK293 cells in vitro.
31396162	0	7	theme	alfa	72:75	arg1	Bioactivity					35:45	in vitro Bioactivity	26:45	in vitro Bioactivity	26:45	Glycosylation Pattern and in vitro Bioactivity of Reference Follitropin alfa and Biosimilars.
31396162	0	7	theme	alfa	72:75	arg1	Pattern					14:20	Glycosylation Pattern	0:20	Glycosylation Pattern	0:20	Glycosylation Pattern and in vitro Bioactivity of Reference Follitropin alfa and Biosimilars.
31396162	10	8	theme	p	1888:1888	arg1	>					1890:1890	p > 0.05	1888:1895	p > 0.05	1888:1895	All preparations induced both 8 and 24 h-progesterone and estradiol synthesis in hGLC, while no different EC50s were demonstrated (Kruskal-Wallis test; p > 0.05; n = 5).
31396162	11	9	theme	similar	2100:2106	arg1	bioactivity					2108:2118	similar bioactivity	2100:2118	similar bioactivity	2100:2118	Apart from preparation-specific intracellular Ca2+ increases achieved at supra-physiological hormone doses, all compounds induced similar intracellular responses and steroidogenesis, reflecting similar bioactivity, and overall structural homogeneity.
31396162	4	10	theme	Kruskal-Wallis	629:642	arg1	test					644:647	Kruskal-Wallis test	629:647	Kruskal-Wallis test	629:647	Gonadotropin N-glycosylation profiles were analyzed by ELISA lectin assay, revealing preparation specific-patterns of glycan species (Kruskal-Wallis test; p < 0.05, n = 6) and by glycotope mapping.
31396162	5	11	theme	-transfected	860:871	arg1	cells					880:884	FSH receptor (FSHR)-transfected HEK293 cells	841:884	FSH receptor (FSHR)-transfected HEK293 cells	841:884	Increasing concentrations of Gonal-f® or biosimilar (1 × 10-3-1 × 103 ng/ml) were used for treating human primary granulosa lutein cells (hGLC) and FSH receptor (FSHR)-transfected HEK293 cells in vitro.
31396162	2	12	theme	source	300:305	arg1	cells					307:311	source cells	300:311	source cells	300:311	They have specific FSH activity and a unique glycosylation profile dependent on source cells.
31396162	8	13	from	production	1312:1321	arg1	cells					1379:1383	FSHR-transfected HEK293 cells	1355:1383	FSHR-transfected HEK293 cells	1355:1383	We found preparation-specific glycosylation patterns by lectin assay (Kruskal-Wallis test; p < 0.001; n = 6), and similar cAMP production and β-arrestin 2 recruitment in FSHR-transfected HEK293 cells (cAMP EC50 range = 12 ± 0.9-24 ± 1.7 ng/ml; β-arrestin 2 EC50 range = 140 ± 14.1-313 ± 18.7 ng/ml; Kruskal-Wallis test; p ≥ 0.05; n = 4).
31396162	1	14	theme	clinical	206:213	arg1	use					215:217	clinical use	206:217	clinical use	206:217	Recombinant follicle-stimulating hormone (FSH) (follitropin alfa) and biosimilar preparations are available for clinical use.
31396162	9	15	theme	Kinetics	1523:1530	arg1	analysis					1532:1539	Kinetics analysis	1523:1539	Kinetics analysis	1523:1539	Kinetics analysis revealed that intracellular Ca2+ increased upon cell treatment by 4 μg/ml Gonal-f®, while equal concentrations of biosimilars failed to induced a response (Kruskal-Wallis test; p < 0.05; n = 3).
31396162	11	16	theme	structural	2133:2142	arg1	homogeneity					2144:2154	overall structural homogeneity	2125:2154	overall structural homogeneity	2125:2154	Apart from preparation-specific intracellular Ca2+ increases achieved at supra-physiological hormone doses, all compounds induced similar intracellular responses and steroidogenesis, reflecting similar bioactivity, and overall structural homogeneity.
31396162	8	17	theme	preparation-specific	1194:1213	arg1	patterns					1229:1236	preparation-specific glycosylation patterns	1194:1236	preparation-specific glycosylation patterns by lectin assay (Kruskal-Wallis test; p < 0.001; n = 6)	1194:1292	We found preparation-specific glycosylation patterns by lectin assay (Kruskal-Wallis test; p < 0.001; n = 6), and similar cAMP production and β-arrestin 2 recruitment in FSHR-transfected HEK293 cells (cAMP EC50 range = 12 ± 0.9-24 ± 1.7 ng/ml; β-arrestin 2 EC50 range = 140 ± 14.1-313 ± 18.7 ng/ml; Kruskal-Wallis test; p ≥ 0.05; n = 4).
31396162	2	18	theme	specific	230:237	arg1	activity					243:250	specific FSH activity	230:250	specific FSH activity	230:250	They have specific FSH activity and a unique glycosylation profile dependent on source cells.
31396162	9	19	theme	μg/ml	1609:1613	arg1	Gonal-f®					1615:1622	4 μg/ml Gonal-f®	1607:1622	4 μg/ml Gonal-f®	1607:1622	Kinetics analysis revealed that intracellular Ca2+ increased upon cell treatment by 4 μg/ml Gonal-f®, while equal concentrations of biosimilars failed to induced a response (Kruskal-Wallis test; p < 0.05; n = 3).
31396162	5	20	theme	human	793:797	arg1	hGLC					831:834	hGLC	831:834	hGLC	831:834	Increasing concentrations of Gonal-f® or biosimilar (1 × 10-3-1 × 103 ng/ml) were used for treating human primary granulosa lutein cells (hGLC) and FSH receptor (FSHR)-transfected HEK293 cells in vitro.
31396162	5	20	theme	human	793:797	arg1	cells					824:828	human primary granulosa lutein cells	793:828	human primary granulosa lutein cells (hGLC)	793:835	Increasing concentrations of Gonal-f® or biosimilar (1 × 10-3-1 × 103 ng/ml) were used for treating human primary granulosa lutein cells (hGLC) and FSH receptor (FSHR)-transfected HEK293 cells in vitro.
31396162	4	21	dep	species	620:626	arg1	=					662:662	=	662:662	=	662:662	Gonadotropin N-glycosylation profiles were analyzed by ELISA lectin assay, revealing preparation specific-patterns of glycan species (Kruskal-Wallis test; p < 0.05, n = 6) and by glycotope mapping.
31396162	5	22	theme	receptor	845:852	arg1	cells					880:884	FSH receptor (FSHR)-transfected HEK293 cells	841:884	FSH receptor (FSHR)-transfected HEK293 cells	841:884	Increasing concentrations of Gonal-f® or biosimilar (1 × 10-3-1 × 103 ng/ml) were used for treating human primary granulosa lutein cells (hGLC) and FSH receptor (FSHR)-transfected HEK293 cells in vitro.
31396162	9	23	theme	intracellular	1555:1567	arg1	Ca2+					1569:1572	intracellular Ca2+	1555:1572	intracellular Ca2+	1555:1572	Kinetics analysis revealed that intracellular Ca2+ increased upon cell treatment by 4 μg/ml Gonal-f®, while equal concentrations of biosimilars failed to induced a response (Kruskal-Wallis test; p < 0.05; n = 3).
31396162	8	24	theme	HEK293	1372:1377	arg1	cells					1379:1383	FSHR-transfected HEK293 cells	1355:1383	FSHR-transfected HEK293 cells	1355:1383	We found preparation-specific glycosylation patterns by lectin assay (Kruskal-Wallis test; p < 0.001; n = 6), and similar cAMP production and β-arrestin 2 recruitment in FSHR-transfected HEK293 cells (cAMP EC50 range = 12 ± 0.9-24 ± 1.7 ng/ml; β-arrestin 2 EC50 range = 140 ± 14.1-313 ± 18.7 ng/ml; Kruskal-Wallis test; p ≥ 0.05; n = 4).
31396162	8	25	theme	lectin	1241:1246	arg1	assay					1248:1252	lectin assay	1241:1252	lectin assay (Kruskal-Wallis test; p < 0.001; n = 6)	1241:1292	We found preparation-specific glycosylation patterns by lectin assay (Kruskal-Wallis test; p < 0.001; n = 6), and similar cAMP production and β-arrestin 2 recruitment in FSHR-transfected HEK293 cells (cAMP EC50 range = 12 ± 0.9-24 ± 1.7 ng/ml; β-arrestin 2 EC50 range = 140 ± 14.1-313 ± 18.7 ng/ml; Kruskal-Wallis test; p ≥ 0.05; n = 4).
31396162	5	26	theme	HEK293	873:878	arg1	cells					880:884	FSH receptor (FSHR)-transfected HEK293 cells	841:884	FSH receptor (FSHR)-transfected HEK293 cells	841:884	Increasing concentrations of Gonal-f® or biosimilar (1 × 10-3-1 × 103 ng/ml) were used for treating human primary granulosa lutein cells (hGLC) and FSH receptor (FSHR)-transfected HEK293 cells in vitro.
31396162	6	27	theme	β-arrestin	945:954	arg1	recruitment					958:968	β-arrestin 2 recruitment	945:968	β-arrestin 2 recruitment	945:968	Intracellular cAMP production, Ca2+ increase and β-arrestin 2 recruitment were evaluated by BRET, CREB, and ERK1/2 phosphorylation by Western blotting.
31396162	11	28	theme	hormone	1999:2005	arg1	doses					2007:2011	supra-physiological hormone doses	1979:2011	supra-physiological hormone doses	1979:2011	Apart from preparation-specific intracellular Ca2+ increases achieved at supra-physiological hormone doses, all compounds induced similar intracellular responses and steroidogenesis, reflecting similar bioactivity, and overall structural homogeneity.
31396162	10	29	theme	estradiol	1794:1802	arg1	synthesis					1804:1812	estradiol synthesis	1794:1812	estradiol synthesis	1794:1812	All preparations induced both 8 and 24 h-progesterone and estradiol synthesis in hGLC, while no different EC50s were demonstrated (Kruskal-Wallis test; p > 0.05; n = 5).
31396162	0	30	theme	Follitropin	60:70	arg1	alfa					72:75	Reference Follitropin alfa	50:75	Reference Follitropin alfa	50:75	Glycosylation Pattern and in vitro Bioactivity of Reference Follitropin alfa and Biosimilars.
31396162	9	31	dep	=	1730:1730	arg1	test					1712:1715	Kruskal-Wallis test	1697:1715	Kruskal-Wallis test	1697:1715	Kinetics analysis revealed that intracellular Ca2+ increased upon cell treatment by 4 μg/ml Gonal-f®, while equal concentrations of biosimilars failed to induced a response (Kruskal-Wallis test; p < 0.05; n = 3).
31396162	9	31	dep	=	1730:1730	arg1	<					1720:1720	p < 0.05	1718:1725	p < 0.05	1718:1725	Kinetics analysis revealed that intracellular Ca2+ increased upon cell treatment by 4 μg/ml Gonal-f®, while equal concentrations of biosimilars failed to induced a response (Kruskal-Wallis test; p < 0.05; n = 3).
31396162	9	32	theme	cell	1589:1592	arg1	treatment					1594:1602	cell treatment	1589:1602	cell treatment	1589:1602	Kinetics analysis revealed that intracellular Ca2+ increased upon cell treatment by 4 μg/ml Gonal-f®, while equal concentrations of biosimilars failed to induced a response (Kruskal-Wallis test; p < 0.05; n = 3).
31396162	8	33	theme	EC50	1391:1394	arg1	ng/ml					1422:1426	cAMP EC50 range = 12 ± 0.9-24 ± 1.7 ng/ml	1386:1426	cAMP EC50 range = 12 ± 0.9-24 ± 1.7 ng/ml	1386:1426	We found preparation-specific glycosylation patterns by lectin assay (Kruskal-Wallis test; p < 0.001; n = 6), and similar cAMP production and β-arrestin 2 recruitment in FSHR-transfected HEK293 cells (cAMP EC50 range = 12 ± 0.9-24 ± 1.7 ng/ml; β-arrestin 2 EC50 range = 140 ± 14.1-313 ± 18.7 ng/ml; Kruskal-Wallis test; p ≥ 0.05; n = 4).
31396162	11	34	theme	intracellular	2044:2056	arg1	responses					2058:2066	similar intracellular responses	2036:2066	similar intracellular responses	2036:2066	Apart from preparation-specific intracellular Ca2+ increases achieved at supra-physiological hormone doses, all compounds induced similar intracellular responses and steroidogenesis, reflecting similar bioactivity, and overall structural homogeneity.
31396162	9	35	dep	response	1687:1694	arg1	=					1730:1730	=	1730:1730	=	1730:1730	Kinetics analysis revealed that intracellular Ca2+ increased upon cell treatment by 4 μg/ml Gonal-f®, while equal concentrations of biosimilars failed to induced a response (Kruskal-Wallis test; p < 0.05; n = 3).
31396162	8	36	theme	=	1402:1402	arg1	ng/ml					1422:1426	cAMP EC50 range = 12 ± 0.9-24 ± 1.7 ng/ml	1386:1426	cAMP EC50 range = 12 ± 0.9-24 ± 1.7 ng/ml	1386:1426	We found preparation-specific glycosylation patterns by lectin assay (Kruskal-Wallis test; p < 0.001; n = 6), and similar cAMP production and β-arrestin 2 recruitment in FSHR-transfected HEK293 cells (cAMP EC50 range = 12 ± 0.9-24 ± 1.7 ng/ml; β-arrestin 2 EC50 range = 140 ± 14.1-313 ± 18.7 ng/ml; Kruskal-Wallis test; p ≥ 0.05; n = 4).
31396162	8	37	theme	p	1276:1276	arg1	<					1278:1278	p < 0.001	1276:1284	p < 0.001	1276:1284	We found preparation-specific glycosylation patterns by lectin assay (Kruskal-Wallis test; p < 0.001; n = 6), and similar cAMP production and β-arrestin 2 recruitment in FSHR-transfected HEK293 cells (cAMP EC50 range = 12 ± 0.9-24 ± 1.7 ng/ml; β-arrestin 2 EC50 range = 140 ± 14.1-313 ± 18.7 ng/ml; Kruskal-Wallis test; p ≥ 0.05; n = 4).
31396162	5	38	theme	Increasing	693:702	arg1	concentrations					704:717	Increasing concentrations	693:717	Increasing concentrations of Gonal-f® or biosimilar (1 × 10-3-1 × 103 ng/ml)	693:768	Increasing concentrations of Gonal-f® or biosimilar (1 × 10-3-1 × 103 ng/ml) were used for treating human primary granulosa lutein cells (hGLC) and FSH receptor (FSHR)-transfected HEK293 cells in vitro.
31396162	8	39	from	patterns	1229:1236	arg1	cells					1379:1383	FSHR-transfected HEK293 cells	1355:1383	FSHR-transfected HEK293 cells	1355:1383	We found preparation-specific glycosylation patterns by lectin assay (Kruskal-Wallis test; p < 0.001; n = 6), and similar cAMP production and β-arrestin 2 recruitment in FSHR-transfected HEK293 cells (cAMP EC50 range = 12 ± 0.9-24 ± 1.7 ng/ml; β-arrestin 2 EC50 range = 140 ± 14.1-313 ± 18.7 ng/ml; Kruskal-Wallis test; p ≥ 0.05; n = 4).
31396162	6	40	theme	cAMP	910:913	arg1	production					915:924	Intracellular cAMP production	896:924	Intracellular cAMP production	896:924	Intracellular cAMP production, Ca2+ increase and β-arrestin 2 recruitment were evaluated by BRET, CREB, and ERK1/2 phosphorylation by Western blotting.
31396162	0	41	theme	Reference	50:58	arg1	alfa					72:75	Reference Follitropin alfa	50:75	Reference Follitropin alfa	50:75	Glycosylation Pattern and in vitro Bioactivity of Reference Follitropin alfa and Biosimilars.
31396162	8	42	theme	±	1407:1407	arg1	ng/ml					1422:1426	cAMP EC50 range = 12 ± 0.9-24 ± 1.7 ng/ml	1386:1426	cAMP EC50 range = 12 ± 0.9-24 ± 1.7 ng/ml	1386:1426	We found preparation-specific glycosylation patterns by lectin assay (Kruskal-Wallis test; p < 0.001; n = 6), and similar cAMP production and β-arrestin 2 recruitment in FSHR-transfected HEK293 cells (cAMP EC50 range = 12 ± 0.9-24 ± 1.7 ng/ml; β-arrestin 2 EC50 range = 140 ± 14.1-313 ± 18.7 ng/ml; Kruskal-Wallis test; p ≥ 0.05; n = 4).
31396162	8	43	from	recruitment	1340:1350	arg1	cells					1379:1383	FSHR-transfected HEK293 cells	1355:1383	FSHR-transfected HEK293 cells	1355:1383	We found preparation-specific glycosylation patterns by lectin assay (Kruskal-Wallis test; p < 0.001; n = 6), and similar cAMP production and β-arrestin 2 recruitment in FSHR-transfected HEK293 cells (cAMP EC50 range = 12 ± 0.9-24 ± 1.7 ng/ml; β-arrestin 2 EC50 range = 140 ± 14.1-313 ± 18.7 ng/ml; Kruskal-Wallis test; p ≥ 0.05; n = 4).
31396162	7	44	theme	estradiol	1103:1111	arg1	synthesis					1113:1121	8- and 24-h progesterone and estradiol synthesis	1074:1121	8- and 24-h progesterone and estradiol synthesis	1074:1121	12-h gene expression, and 8- and 24-h progesterone and estradiol synthesis were measured by real-time PCR and immunoassay, respectively.
31396162	3	45	theme	reference	365:373	arg1	alfa					388:391	the originator (reference) follitropin alfa (Gonal-f®)-	349:403	the originator (reference) follitropin alfa (Gonal-f®)- with biosimilar preparations (Bemfola® and Ovaleap®)-induced cellular responses	349:483	The aim of the study is to compare the originator (reference) follitropin alfa (Gonal-f®)- with biosimilar preparations (Bemfola® and Ovaleap®)-induced cellular responses in vitro.
31396162	8	46	theme	±	1416:1416	arg1	ng/ml					1422:1426	cAMP EC50 range = 12 ± 0.9-24 ± 1.7 ng/ml	1386:1426	cAMP EC50 range = 12 ± 0.9-24 ± 1.7 ng/ml	1386:1426	We found preparation-specific glycosylation patterns by lectin assay (Kruskal-Wallis test; p < 0.001; n = 6), and similar cAMP production and β-arrestin 2 recruitment in FSHR-transfected HEK293 cells (cAMP EC50 range = 12 ± 0.9-24 ± 1.7 ng/ml; β-arrestin 2 EC50 range = 140 ± 14.1-313 ± 18.7 ng/ml; Kruskal-Wallis test; p ≥ 0.05; n = 4).
31396162	11	47	dep	increases	1957:1965	arg1	achieved					1967:1974	achieved	1967:1974	increases achieved at supra-physiological hormone doses, all compounds induced similar intracellular responses and steroidogenesis, reflecting similar bioactivity, and overall structural homogeneity	1957:2154	Apart from preparation-specific intracellular Ca2+ increases achieved at supra-physiological hormone doses, all compounds induced similar intracellular responses and steroidogenesis, reflecting similar bioactivity, and overall structural homogeneity.
31396162	7	48	theme	real-time	1140:1148	arg1	PCR					1150:1152	real-time PCR	1140:1152	real-time PCR	1140:1152	12-h gene expression, and 8- and 24-h progesterone and estradiol synthesis were measured by real-time PCR and immunoassay, respectively.
31396162	0	49	theme	Glycosylation	0:12	arg1	Pattern					14:20	Glycosylation Pattern	0:20	Glycosylation Pattern	0:20	Glycosylation Pattern and in vitro Bioactivity of Reference Follitropin alfa and Biosimilars.
31396162	11	50	theme	intracellular	1938:1950	arg1	Ca2+					1952:1955	preparation-specific intracellular Ca2+	1917:1955	preparation-specific intracellular Ca2+	1917:1955	Apart from preparation-specific intracellular Ca2+ increases achieved at supra-physiological hormone doses, all compounds induced similar intracellular responses and steroidogenesis, reflecting similar bioactivity, and overall structural homogeneity.
31396162	1	51	theme	Recombinant	94:104	arg1	alfa					154:157	follitropin alfa	142:157	follitropin alfa	142:157	Recombinant follicle-stimulating hormone (FSH) (follitropin alfa) and biosimilar preparations are available for clinical use.
31396162	1	51	theme	Recombinant	94:104	arg1	FSH					136:138	FSH	136:138	FSH	136:138	Recombinant follicle-stimulating hormone (FSH) (follitropin alfa) and biosimilar preparations are available for clinical use.
31396162	1	51	theme	Recombinant	94:104	arg1	hormone					127:133	Recombinant follicle-stimulating hormone	94:133	Recombinant follicle-stimulating hormone (FSH) (follitropin alfa)	94:158	Recombinant follicle-stimulating hormone (FSH) (follitropin alfa) and biosimilar preparations are available for clinical use.
31396162	3	52	theme	originator	353:362	arg1	alfa					388:391	the originator (reference) follitropin alfa (Gonal-f®)-	349:403	the originator (reference) follitropin alfa (Gonal-f®)- with biosimilar preparations (Bemfola® and Ovaleap®)-induced cellular responses	349:483	The aim of the study is to compare the originator (reference) follitropin alfa (Gonal-f®)- with biosimilar preparations (Bemfola® and Ovaleap®)-induced cellular responses in vitro.
31396162	9	53	theme	equal	1631:1635	arg1	concentrations					1637:1650	equal concentrations	1631:1650	equal concentrations of biosimilars	1631:1665	Kinetics analysis revealed that intracellular Ca2+ increased upon cell treatment by 4 μg/ml Gonal-f®, while equal concentrations of biosimilars failed to induced a response (Kruskal-Wallis test; p < 0.05; n = 3).
31396162	3	54	theme	Bemfola®	435:442	arg1	responses					475:483	the originator (reference) follitropin alfa (Gonal-f®)- with biosimilar preparations (Bemfola® and Ovaleap®)-induced cellular responses	349:483	the originator (reference) follitropin alfa (Gonal-f®)- with biosimilar preparations (Bemfola® and Ovaleap®)-induced cellular responses	349:483	The aim of the study is to compare the originator (reference) follitropin alfa (Gonal-f®)- with biosimilar preparations (Bemfola® and Ovaleap®)-induced cellular responses in vitro.
31396162	4	55	theme	ELISA	550:554	arg1	assay					563:567	ELISA lectin assay	550:567	ELISA lectin assay	550:567	Gonadotropin N-glycosylation profiles were analyzed by ELISA lectin assay, revealing preparation specific-patterns of glycan species (Kruskal-Wallis test; p < 0.05, n = 6) and by glycotope mapping.
31396162	7	56	theme	progesterone	1086:1097	arg1	synthesis					1113:1121	8- and 24-h progesterone and estradiol synthesis	1074:1121	8- and 24-h progesterone and estradiol synthesis	1074:1121	12-h gene expression, and 8- and 24-h progesterone and estradiol synthesis were measured by real-time PCR and immunoassay, respectively.
31396162	1	57	theme	biosimilar	164:173	arg1	preparations					175:186	biosimilar preparations	164:186	biosimilar preparations	164:186	Recombinant follicle-stimulating hormone (FSH) (follitropin alfa) and biosimilar preparations are available for clinical use.
31396162	5	58	theme	×	748:748	arg1	ng/ml					763:767	1 × 10-3-1 × 103 ng/ml	746:767	1 × 10-3-1 × 103 ng/ml	746:767	Increasing concentrations of Gonal-f® or biosimilar (1 × 10-3-1 × 103 ng/ml) were used for treating human primary granulosa lutein cells (hGLC) and FSH receptor (FSHR)-transfected HEK293 cells in vitro.
31396162	5	58	theme	×	748:748	arg1	Gonal-f®					722:729	Gonal-f®	722:729	Gonal-f®	722:729	Increasing concentrations of Gonal-f® or biosimilar (1 × 10-3-1 × 103 ng/ml) were used for treating human primary granulosa lutein cells (hGLC) and FSH receptor (FSHR)-transfected HEK293 cells in vitro.
31396162	10	59	dep	=	1900:1900	arg1	test					1882:1885	Kruskal-Wallis test	1867:1885	Kruskal-Wallis test	1867:1885	All preparations induced both 8 and 24 h-progesterone and estradiol synthesis in hGLC, while no different EC50s were demonstrated (Kruskal-Wallis test; p > 0.05; n = 5).
31396162	10	59	dep	=	1900:1900	arg1	>					1890:1890	p > 0.05	1888:1895	p > 0.05	1888:1895	All preparations induced both 8 and 24 h-progesterone and estradiol synthesis in hGLC, while no different EC50s were demonstrated (Kruskal-Wallis test; p > 0.05; n = 5).
31396162	3	60	theme	follitropin	376:386	arg1	alfa					388:391	the originator (reference) follitropin alfa (Gonal-f®)-	349:403	the originator (reference) follitropin alfa (Gonal-f®)- with biosimilar preparations (Bemfola® and Ovaleap®)-induced cellular responses	349:483	The aim of the study is to compare the originator (reference) follitropin alfa (Gonal-f®)- with biosimilar preparations (Bemfola® and Ovaleap®)-induced cellular responses in vitro.
31396162	8	61	theme	EC50	1442:1445	arg1	range					1447:1451	β-arrestin 2 EC50 range	1429:1451	β-arrestin 2 EC50 range	1429:1451	We found preparation-specific glycosylation patterns by lectin assay (Kruskal-Wallis test; p < 0.001; n = 6), and similar cAMP production and β-arrestin 2 recruitment in FSHR-transfected HEK293 cells (cAMP EC50 range = 12 ± 0.9-24 ± 1.7 ng/ml; β-arrestin 2 EC50 range = 140 ± 14.1-313 ± 18.7 ng/ml; Kruskal-Wallis test; p ≥ 0.05; n = 4).
31396162	8	62	theme	similar	1299:1305	arg1	production					1312:1321	similar cAMP production	1299:1321	similar cAMP production	1299:1321	We found preparation-specific glycosylation patterns by lectin assay (Kruskal-Wallis test; p < 0.001; n = 6), and similar cAMP production and β-arrestin 2 recruitment in FSHR-transfected HEK293 cells (cAMP EC50 range = 12 ± 0.9-24 ± 1.7 ng/ml; β-arrestin 2 EC50 range = 140 ± 14.1-313 ± 18.7 ng/ml; Kruskal-Wallis test; p ≥ 0.05; n = 4).
31396162	3	63	theme	-induced	457:464	arg1	responses					475:483	the originator (reference) follitropin alfa (Gonal-f®)- with biosimilar preparations (Bemfola® and Ovaleap®)-induced cellular responses	349:483	the originator (reference) follitropin alfa (Gonal-f®)- with biosimilar preparations (Bemfola® and Ovaleap®)-induced cellular responses	349:483	The aim of the study is to compare the originator (reference) follitropin alfa (Gonal-f®)- with biosimilar preparations (Bemfola® and Ovaleap®)-induced cellular responses in vitro.
31396162	5	64	theme	×	757:757	arg1	ng/ml					763:767	1 × 10-3-1 × 103 ng/ml	746:767	1 × 10-3-1 × 103 ng/ml	746:767	Increasing concentrations of Gonal-f® or biosimilar (1 × 10-3-1 × 103 ng/ml) were used for treating human primary granulosa lutein cells (hGLC) and FSH receptor (FSHR)-transfected HEK293 cells in vitro.
31396162	5	64	theme	×	757:757	arg1	Gonal-f®					722:729	Gonal-f®	722:729	Gonal-f®	722:729	Increasing concentrations of Gonal-f® or biosimilar (1 × 10-3-1 × 103 ng/ml) were used for treating human primary granulosa lutein cells (hGLC) and FSH receptor (FSHR)-transfected HEK293 cells in vitro.
31396162	8	65	dep	assay	1248:1252	arg1	=					1289:1289	=	1289:1289	=	1289:1289	We found preparation-specific glycosylation patterns by lectin assay (Kruskal-Wallis test; p < 0.001; n = 6), and similar cAMP production and β-arrestin 2 recruitment in FSHR-transfected HEK293 cells (cAMP EC50 range = 12 ± 0.9-24 ± 1.7 ng/ml; β-arrestin 2 EC50 range = 140 ± 14.1-313 ± 18.7 ng/ml; Kruskal-Wallis test; p ≥ 0.05; n = 4).
31396162	8	66	dep	=	1289:1289	arg1	<					1278:1278	p < 0.001	1276:1284	p < 0.001	1276:1284	We found preparation-specific glycosylation patterns by lectin assay (Kruskal-Wallis test; p < 0.001; n = 6), and similar cAMP production and β-arrestin 2 recruitment in FSHR-transfected HEK293 cells (cAMP EC50 range = 12 ± 0.9-24 ± 1.7 ng/ml; β-arrestin 2 EC50 range = 140 ± 14.1-313 ± 18.7 ng/ml; Kruskal-Wallis test; p ≥ 0.05; n = 4).
31396162	8	66	dep	=	1289:1289	arg1	test					1270:1273	Kruskal-Wallis test	1255:1273	Kruskal-Wallis test	1255:1273	We found preparation-specific glycosylation patterns by lectin assay (Kruskal-Wallis test; p < 0.001; n = 6), and similar cAMP production and β-arrestin 2 recruitment in FSHR-transfected HEK293 cells (cAMP EC50 range = 12 ± 0.9-24 ± 1.7 ng/ml; β-arrestin 2 EC50 range = 140 ± 14.1-313 ± 18.7 ng/ml; Kruskal-Wallis test; p ≥ 0.05; n = 4).
31396162	3	67	theme	Ovaleap®	448:455	arg1	responses					475:483	the originator (reference) follitropin alfa (Gonal-f®)- with biosimilar preparations (Bemfola® and Ovaleap®)-induced cellular responses	349:483	the originator (reference) follitropin alfa (Gonal-f®)- with biosimilar preparations (Bemfola® and Ovaleap®)-induced cellular responses	349:483	The aim of the study is to compare the originator (reference) follitropin alfa (Gonal-f®)- with biosimilar preparations (Bemfola® and Ovaleap®)-induced cellular responses in vitro.
31396162	5	68	used	used	775:778	arg2	concentrations					704:717	Increasing concentrations	693:717	Increasing concentrations of Gonal-f® or biosimilar (1 × 10-3-1 × 103 ng/ml)	693:768	Increasing concentrations of Gonal-f® or biosimilar (1 × 10-3-1 × 103 ng/ml) were used for treating human primary granulosa lutein cells (hGLC) and FSH receptor (FSHR)-transfected HEK293 cells in vitro.
31396162	6	69	theme	Western	1030:1036	arg1	blotting					1038:1045	Western blotting	1030:1045	Western blotting	1030:1045	Intracellular cAMP production, Ca2+ increase and β-arrestin 2 recruitment were evaluated by BRET, CREB, and ERK1/2 phosphorylation by Western blotting.
31396162	8	70	theme	±	1459:1459	arg1	ng/ml					1477:1481	140 ± 14.1-313 ± 18.7 ng/ml	1455:1481	140 ± 14.1-313 ± 18.7 ng/ml	1455:1481	We found preparation-specific glycosylation patterns by lectin assay (Kruskal-Wallis test; p < 0.001; n = 6), and similar cAMP production and β-arrestin 2 recruitment in FSHR-transfected HEK293 cells (cAMP EC50 range = 12 ± 0.9-24 ± 1.7 ng/ml; β-arrestin 2 EC50 range = 140 ± 14.1-313 ± 18.7 ng/ml; Kruskal-Wallis test; p ≥ 0.05; n = 4).
31396162	8	71	theme	β-arrestin	1327:1336	arg1	recruitment					1340:1350	β-arrestin 2 recruitment	1327:1350	β-arrestin 2 recruitment	1327:1350	We found preparation-specific glycosylation patterns by lectin assay (Kruskal-Wallis test; p < 0.001; n = 6), and similar cAMP production and β-arrestin 2 recruitment in FSHR-transfected HEK293 cells (cAMP EC50 range = 12 ± 0.9-24 ± 1.7 ng/ml; β-arrestin 2 EC50 range = 140 ± 14.1-313 ± 18.7 ng/ml; Kruskal-Wallis test; p ≥ 0.05; n = 4).
31396162	8	72	theme	≥	1507:1507	arg1	p					1505:1505	p ≥ 0.05	1505:1512	p ≥ 0.05	1505:1512	We found preparation-specific glycosylation patterns by lectin assay (Kruskal-Wallis test; p < 0.001; n = 6), and similar cAMP production and β-arrestin 2 recruitment in FSHR-transfected HEK293 cells (cAMP EC50 range = 12 ± 0.9-24 ± 1.7 ng/ml; β-arrestin 2 EC50 range = 140 ± 14.1-313 ± 18.7 ng/ml; Kruskal-Wallis test; p ≥ 0.05; n = 4).
31396162	7	73	theme	gene	1053:1056	arg1	expression					1058:1067	12-h gene expression	1048:1067	12-h gene expression	1048:1067	12-h gene expression, and 8- and 24-h progesterone and estradiol synthesis were measured by real-time PCR and immunoassay, respectively.
31396162	9	74	theme	Kruskal-Wallis	1697:1710	arg1	test					1712:1715	Kruskal-Wallis test	1697:1715	Kruskal-Wallis test	1697:1715	Kinetics analysis revealed that intracellular Ca2+ increased upon cell treatment by 4 μg/ml Gonal-f®, while equal concentrations of biosimilars failed to induced a response (Kruskal-Wallis test; p < 0.05; n = 3).
31396162	8	75	theme	±	1470:1470	arg1	ng/ml					1477:1481	140 ± 14.1-313 ± 18.7 ng/ml	1455:1481	140 ± 14.1-313 ± 18.7 ng/ml	1455:1481	We found preparation-specific glycosylation patterns by lectin assay (Kruskal-Wallis test; p < 0.001; n = 6), and similar cAMP production and β-arrestin 2 recruitment in FSHR-transfected HEK293 cells (cAMP EC50 range = 12 ± 0.9-24 ± 1.7 ng/ml; β-arrestin 2 EC50 range = 140 ± 14.1-313 ± 18.7 ng/ml; Kruskal-Wallis test; p ≥ 0.05; n = 4).
31396162	5	76	theme	biosimilar	734:743	arg1	concentrations					704:717	Increasing concentrations	693:717	Increasing concentrations of Gonal-f® or biosimilar (1 × 10-3-1 × 103 ng/ml)	693:768	Increasing concentrations of Gonal-f® or biosimilar (1 × 10-3-1 × 103 ng/ml) were used for treating human primary granulosa lutein cells (hGLC) and FSH receptor (FSHR)-transfected HEK293 cells in vitro.
31396162	10	77	theme	Kruskal-Wallis	1867:1880	arg1	test					1882:1885	Kruskal-Wallis test	1867:1885	Kruskal-Wallis test	1867:1885	All preparations induced both 8 and 24 h-progesterone and estradiol synthesis in hGLC, while no different EC50s were demonstrated (Kruskal-Wallis test; p > 0.05; n = 5).
31396162	8	78	theme	=	1517:1517	arg1	n					1515:1515	n = 4	1515:1519	n = 4	1515:1519	We found preparation-specific glycosylation patterns by lectin assay (Kruskal-Wallis test; p < 0.001; n = 6), and similar cAMP production and β-arrestin 2 recruitment in FSHR-transfected HEK293 cells (cAMP EC50 range = 12 ± 0.9-24 ± 1.7 ng/ml; β-arrestin 2 EC50 range = 140 ± 14.1-313 ± 18.7 ng/ml; Kruskal-Wallis test; p ≥ 0.05; n = 4).
31396162	3	79	with	alfa	388:391	arg1	preparations					421:432	biosimilar preparations	410:432	biosimilar preparations	410:432	The aim of the study is to compare the originator (reference) follitropin alfa (Gonal-f®)- with biosimilar preparations (Bemfola® and Ovaleap®)-induced cellular responses in vitro.
31396162	8	80	theme	Kruskal-Wallis	1484:1497	arg1	test					1499:1502	Kruskal-Wallis test	1484:1502	Kruskal-Wallis test	1484:1502	We found preparation-specific glycosylation patterns by lectin assay (Kruskal-Wallis test; p < 0.001; n = 6), and similar cAMP production and β-arrestin 2 recruitment in FSHR-transfected HEK293 cells (cAMP EC50 range = 12 ± 0.9-24 ± 1.7 ng/ml; β-arrestin 2 EC50 range = 140 ± 14.1-313 ± 18.7 ng/ml; Kruskal-Wallis test; p ≥ 0.05; n = 4).
31396162	5	81	theme	primary	799:805	arg1	hGLC					831:834	hGLC	831:834	hGLC	831:834	Increasing concentrations of Gonal-f® or biosimilar (1 × 10-3-1 × 103 ng/ml) were used for treating human primary granulosa lutein cells (hGLC) and FSH receptor (FSHR)-transfected HEK293 cells in vitro.
31396162	5	81	theme	primary	799:805	arg1	cells					824:828	human primary granulosa lutein cells	793:828	human primary granulosa lutein cells (hGLC)	793:835	Increasing concentrations of Gonal-f® or biosimilar (1 × 10-3-1 × 103 ng/ml) were used for treating human primary granulosa lutein cells (hGLC) and FSH receptor (FSHR)-transfected HEK293 cells in vitro.
31396162	11	82	theme	similar	2036:2042	arg1	responses					2058:2066	similar intracellular responses	2036:2066	similar intracellular responses	2036:2066	Apart from preparation-specific intracellular Ca2+ increases achieved at supra-physiological hormone doses, all compounds induced similar intracellular responses and steroidogenesis, reflecting similar bioactivity, and overall structural homogeneity.
31396162	4	83	theme	p	650:650	arg1	<					652:652	p < 0.05	650:657	p < 0.05	650:657	Gonadotropin N-glycosylation profiles were analyzed by ELISA lectin assay, revealing preparation specific-patterns of glycan species (Kruskal-Wallis test; p < 0.05, n = 6) and by glycotope mapping.
31396162	5	84	theme	lutein	817:822	arg1	hGLC					831:834	hGLC	831:834	hGLC	831:834	Increasing concentrations of Gonal-f® or biosimilar (1 × 10-3-1 × 103 ng/ml) were used for treating human primary granulosa lutein cells (hGLC) and FSH receptor (FSHR)-transfected HEK293 cells in vitro.
31396162	5	84	theme	lutein	817:822	arg1	cells					824:828	human primary granulosa lutein cells	793:828	human primary granulosa lutein cells (hGLC)	793:835	Increasing concentrations of Gonal-f® or biosimilar (1 × 10-3-1 × 103 ng/ml) were used for treating human primary granulosa lutein cells (hGLC) and FSH receptor (FSHR)-transfected HEK293 cells in vitro.
31396162	6	85	theme	Intracellular	896:908	arg1	production					915:924	Intracellular cAMP production	896:924	Intracellular cAMP production	896:924	Intracellular cAMP production, Ca2+ increase and β-arrestin 2 recruitment were evaluated by BRET, CREB, and ERK1/2 phosphorylation by Western blotting.
31396162	8	86	dep	=	1453:1453	arg1	n					1515:1515	n = 4	1515:1519	n = 4	1515:1519	We found preparation-specific glycosylation patterns by lectin assay (Kruskal-Wallis test; p < 0.001; n = 6), and similar cAMP production and β-arrestin 2 recruitment in FSHR-transfected HEK293 cells (cAMP EC50 range = 12 ± 0.9-24 ± 1.7 ng/ml; β-arrestin 2 EC50 range = 140 ± 14.1-313 ± 18.7 ng/ml; Kruskal-Wallis test; p ≥ 0.05; n = 4).
31396162	8	86	dep	=	1453:1453	arg1	ng/ml					1422:1426	cAMP EC50 range = 12 ± 0.9-24 ± 1.7 ng/ml	1386:1426	cAMP EC50 range = 12 ± 0.9-24 ± 1.7 ng/ml	1386:1426	We found preparation-specific glycosylation patterns by lectin assay (Kruskal-Wallis test; p < 0.001; n = 6), and similar cAMP production and β-arrestin 2 recruitment in FSHR-transfected HEK293 cells (cAMP EC50 range = 12 ± 0.9-24 ± 1.7 ng/ml; β-arrestin 2 EC50 range = 140 ± 14.1-313 ± 18.7 ng/ml; Kruskal-Wallis test; p ≥ 0.05; n = 4).
31396162	8	86	dep	=	1453:1453	arg1	p					1505:1505	p ≥ 0.05	1505:1512	p ≥ 0.05	1505:1512	We found preparation-specific glycosylation patterns by lectin assay (Kruskal-Wallis test; p < 0.001; n = 6), and similar cAMP production and β-arrestin 2 recruitment in FSHR-transfected HEK293 cells (cAMP EC50 range = 12 ± 0.9-24 ± 1.7 ng/ml; β-arrestin 2 EC50 range = 140 ± 14.1-313 ± 18.7 ng/ml; Kruskal-Wallis test; p ≥ 0.05; n = 4).
31396162	8	86	dep	=	1453:1453	arg1	test					1499:1502	Kruskal-Wallis test	1484:1502	Kruskal-Wallis test	1484:1502	We found preparation-specific glycosylation patterns by lectin assay (Kruskal-Wallis test; p < 0.001; n = 6), and similar cAMP production and β-arrestin 2 recruitment in FSHR-transfected HEK293 cells (cAMP EC50 range = 12 ± 0.9-24 ± 1.7 ng/ml; β-arrestin 2 EC50 range = 140 ± 14.1-313 ± 18.7 ng/ml; Kruskal-Wallis test; p ≥ 0.05; n = 4).
31396162	2	87	theme	FSH	239:241	arg1	activity					243:250	specific FSH activity	230:250	specific FSH activity	230:250	They have specific FSH activity and a unique glycosylation profile dependent on source cells.
31396162	8	88	theme	FSHR-transfected	1355:1370	arg1	cells					1379:1383	FSHR-transfected HEK293 cells	1355:1383	FSHR-transfected HEK293 cells	1355:1383	We found preparation-specific glycosylation patterns by lectin assay (Kruskal-Wallis test; p < 0.001; n = 6), and similar cAMP production and β-arrestin 2 recruitment in FSHR-transfected HEK293 cells (cAMP EC50 range = 12 ± 0.9-24 ± 1.7 ng/ml; β-arrestin 2 EC50 range = 140 ± 14.1-313 ± 18.7 ng/ml; Kruskal-Wallis test; p ≥ 0.05; n = 4).
31396162	5	89	theme	FSH	841:843	arg1	receptor					845:852	FSH receptor	841:852	FSH receptor (FSHR)-transfected HEK293 cells	841:884	Increasing concentrations of Gonal-f® or biosimilar (1 × 10-3-1 × 103 ng/ml) were used for treating human primary granulosa lutein cells (hGLC) and FSH receptor (FSHR)-transfected HEK293 cells in vitro.
31396162	5	89	theme	FSH	841:843	arg1	FSHR					855:858	FSHR	855:858	FSHR	855:858	Increasing concentrations of Gonal-f® or biosimilar (1 × 10-3-1 × 103 ng/ml) were used for treating human primary granulosa lutein cells (hGLC) and FSH receptor (FSHR)-transfected HEK293 cells in vitro.
31396162	8	90	theme	glycosylation	1215:1227	arg1	patterns					1229:1236	preparation-specific glycosylation patterns	1194:1236	preparation-specific glycosylation patterns by lectin assay (Kruskal-Wallis test; p < 0.001; n = 6)	1194:1292	We found preparation-specific glycosylation patterns by lectin assay (Kruskal-Wallis test; p < 0.001; n = 6), and similar cAMP production and β-arrestin 2 recruitment in FSHR-transfected HEK293 cells (cAMP EC50 range = 12 ± 0.9-24 ± 1.7 ng/ml; β-arrestin 2 EC50 range = 140 ± 14.1-313 ± 18.7 ng/ml; Kruskal-Wallis test; p ≥ 0.05; n = 4).
31396162	7	91	theme	8-	1074:1075	arg1	synthesis					1113:1121	8- and 24-h progesterone and estradiol synthesis	1074:1121	8- and 24-h progesterone and estradiol synthesis	1074:1121	12-h gene expression, and 8- and 24-h progesterone and estradiol synthesis were measured by real-time PCR and immunoassay, respectively.
31396162	2	92	theme	unique	258:263	arg1	profile					279:285	a unique glycosylation profile	256:285	a unique glycosylation profile dependent on source cells	256:311	They have specific FSH activity and a unique glycosylation profile dependent on source cells.
31396162	8	93	theme	cAMP	1386:1389	arg1	ng/ml					1422:1426	cAMP EC50 range = 12 ± 0.9-24 ± 1.7 ng/ml	1386:1426	cAMP EC50 range = 12 ± 0.9-24 ± 1.7 ng/ml	1386:1426	We found preparation-specific glycosylation patterns by lectin assay (Kruskal-Wallis test; p < 0.001; n = 6), and similar cAMP production and β-arrestin 2 recruitment in FSHR-transfected HEK293 cells (cAMP EC50 range = 12 ± 0.9-24 ± 1.7 ng/ml; β-arrestin 2 EC50 range = 140 ± 14.1-313 ± 18.7 ng/ml; Kruskal-Wallis test; p ≥ 0.05; n = 4).
31396162	4	94	theme	glycotope	674:682	arg1	mapping					684:690	glycotope mapping	674:690	glycotope mapping	674:690	Gonadotropin N-glycosylation profiles were analyzed by ELISA lectin assay, revealing preparation specific-patterns of glycan species (Kruskal-Wallis test; p < 0.05, n = 6) and by glycotope mapping.
31396162	5	95	theme	Gonal-f®	722:729	arg1	concentrations					704:717	Increasing concentrations	693:717	Increasing concentrations of Gonal-f® or biosimilar (1 × 10-3-1 × 103 ng/ml)	693:768	Increasing concentrations of Gonal-f® or biosimilar (1 × 10-3-1 × 103 ng/ml) were used for treating human primary granulosa lutein cells (hGLC) and FSH receptor (FSHR)-transfected HEK293 cells in vitro.
31396162	1	96	theme	follitropin	142:152	arg1	alfa					154:157	follitropin alfa	142:157	follitropin alfa	142:157	Recombinant follicle-stimulating hormone (FSH) (follitropin alfa) and biosimilar preparations are available for clinical use.
31396162	1	96	theme	follitropin	142:152	arg1	hormone					127:133	Recombinant follicle-stimulating hormone	94:133	Recombinant follicle-stimulating hormone (FSH) (follitropin alfa)	94:158	Recombinant follicle-stimulating hormone (FSH) (follitropin alfa) and biosimilar preparations are available for clinical use.
31396162	3	97	theme	study	329:333	arg1	aim					318:320	The aim	314:320	The aim of the study	314:333	The aim of the study is to compare the originator (reference) follitropin alfa (Gonal-f®)- with biosimilar preparations (Bemfola® and Ovaleap®)-induced cellular responses in vitro.
31396162	8	98	theme	range	1396:1400	arg1	ng/ml					1422:1426	cAMP EC50 range = 12 ± 0.9-24 ± 1.7 ng/ml	1386:1426	cAMP EC50 range = 12 ± 0.9-24 ± 1.7 ng/ml	1386:1426	We found preparation-specific glycosylation patterns by lectin assay (Kruskal-Wallis test; p < 0.001; n = 6), and similar cAMP production and β-arrestin 2 recruitment in FSHR-transfected HEK293 cells (cAMP EC50 range = 12 ± 0.9-24 ± 1.7 ng/ml; β-arrestin 2 EC50 range = 140 ± 14.1-313 ± 18.7 ng/ml; Kruskal-Wallis test; p ≥ 0.05; n = 4).
31396162	0	99	theme	Biosimilars	81:91	arg1	Bioactivity					35:45	in vitro Bioactivity	26:45	in vitro Bioactivity	26:45	Glycosylation Pattern and in vitro Bioactivity of Reference Follitropin alfa and Biosimilars.
31396162	0	99	theme	Biosimilars	81:91	arg1	Pattern					14:20	Glycosylation Pattern	0:20	Glycosylation Pattern	0:20	Glycosylation Pattern and in vitro Bioactivity of Reference Follitropin alfa and Biosimilars.
31396162	6	100	theme	Ca2+	927:930	arg1	increase					932:939	Ca2+ increase	927:939	Ca2+ increase	927:939	Intracellular cAMP production, Ca2+ increase and β-arrestin 2 recruitment were evaluated by BRET, CREB, and ERK1/2 phosphorylation by Western blotting.
31396162	11	101	theme	supra-physiological	1979:1997	arg1	doses					2007:2011	supra-physiological hormone doses	1979:2011	supra-physiological hormone doses	1979:2011	Apart from preparation-specific intracellular Ca2+ increases achieved at supra-physiological hormone doses, all compounds induced similar intracellular responses and steroidogenesis, reflecting similar bioactivity, and overall structural homogeneity.
31396162	8	102	theme	Kruskal-Wallis	1255:1268	arg1	test					1270:1273	Kruskal-Wallis test	1255:1273	Kruskal-Wallis test	1255:1273	We found preparation-specific glycosylation patterns by lectin assay (Kruskal-Wallis test; p < 0.001; n = 6), and similar cAMP production and β-arrestin 2 recruitment in FSHR-transfected HEK293 cells (cAMP EC50 range = 12 ± 0.9-24 ± 1.7 ng/ml; β-arrestin 2 EC50 range = 140 ± 14.1-313 ± 18.7 ng/ml; Kruskal-Wallis test; p ≥ 0.05; n = 4).
31396162	4	103	dep	=	662:662	arg1	test					644:647	Kruskal-Wallis test	629:647	Kruskal-Wallis test	629:647	Gonadotropin N-glycosylation profiles were analyzed by ELISA lectin assay, revealing preparation specific-patterns of glycan species (Kruskal-Wallis test; p < 0.05, n = 6) and by glycotope mapping.
31396162	4	103	dep	=	662:662	arg1	<					652:652	p < 0.05	650:657	p < 0.05	650:657	Gonadotropin N-glycosylation profiles were analyzed by ELISA lectin assay, revealing preparation specific-patterns of glycan species (Kruskal-Wallis test; p < 0.05, n = 6) and by glycotope mapping.
31396162	3	104	theme	biosimilar	410:419	arg1	preparations					421:432	biosimilar preparations	410:432	biosimilar preparations	410:432	The aim of the study is to compare the originator (reference) follitropin alfa (Gonal-f®)- with biosimilar preparations (Bemfola® and Ovaleap®)-induced cellular responses in vitro.
31396162	7	105	theme	24-h	1081:1084	arg1	synthesis					1113:1121	8- and 24-h progesterone and estradiol synthesis	1074:1121	8- and 24-h progesterone and estradiol synthesis	1074:1121	12-h gene expression, and 8- and 24-h progesterone and estradiol synthesis were measured by real-time PCR and immunoassay, respectively.
31396162	11	106	theme	preparation-specific	1917:1936	arg1	Ca2+					1952:1955	preparation-specific intracellular Ca2+	1917:1955	preparation-specific intracellular Ca2+	1917:1955	Apart from preparation-specific intracellular Ca2+ increases achieved at supra-physiological hormone doses, all compounds induced similar intracellular responses and steroidogenesis, reflecting similar bioactivity, and overall structural homogeneity.
31396162	0	107	theme	in	26:27	arg1	Bioactivity					35:45	in vitro Bioactivity	26:45	in vitro Bioactivity	26:45	Glycosylation Pattern and in vitro Bioactivity of Reference Follitropin alfa and Biosimilars.
31396162	1	108	theme	follicle-stimulating	106:125	arg1	alfa					154:157	follitropin alfa	142:157	follitropin alfa	142:157	Recombinant follicle-stimulating hormone (FSH) (follitropin alfa) and biosimilar preparations are available for clinical use.
31396162	1	108	theme	follicle-stimulating	106:125	arg1	FSH					136:138	FSH	136:138	FSH	136:138	Recombinant follicle-stimulating hormone (FSH) (follitropin alfa) and biosimilar preparations are available for clinical use.
31396162	1	108	theme	follicle-stimulating	106:125	arg1	hormone					127:133	Recombinant follicle-stimulating hormone	94:133	Recombinant follicle-stimulating hormone (FSH) (follitropin alfa)	94:158	Recombinant follicle-stimulating hormone (FSH) (follitropin alfa) and biosimilar preparations are available for clinical use.
31396162	4	109	theme	lectin	556:561	arg1	assay					563:567	ELISA lectin assay	550:567	ELISA lectin assay	550:567	Gonadotropin N-glycosylation profiles were analyzed by ELISA lectin assay, revealing preparation specific-patterns of glycan species (Kruskal-Wallis test; p < 0.05, n = 6) and by glycotope mapping.
31396162	9	110	theme	biosimilars	1655:1665	arg1	concentrations					1637:1650	equal concentrations	1631:1650	equal concentrations of biosimilars	1631:1665	Kinetics analysis revealed that intracellular Ca2+ increased upon cell treatment by 4 μg/ml Gonal-f®, while equal concentrations of biosimilars failed to induced a response (Kruskal-Wallis test; p < 0.05; n = 3).
31396162	4	111	theme	N-glycosylation	508:522	arg1	profiles					524:531	Gonadotropin N-glycosylation profiles	495:531	Gonadotropin N-glycosylation profiles	495:531	Gonadotropin N-glycosylation profiles were analyzed by ELISA lectin assay, revealing preparation specific-patterns of glycan species (Kruskal-Wallis test; p < 0.05, n = 6) and by glycotope mapping.
31396162	5	112	theme	10-3-1	750:755	arg1	ng/ml					763:767	1 × 10-3-1 × 103 ng/ml	746:767	1 × 10-3-1 × 103 ng/ml	746:767	Increasing concentrations of Gonal-f® or biosimilar (1 × 10-3-1 × 103 ng/ml) were used for treating human primary granulosa lutein cells (hGLC) and FSH receptor (FSHR)-transfected HEK293 cells in vitro.
31396162	5	112	theme	10-3-1	750:755	arg1	Gonal-f®					722:729	Gonal-f®	722:729	Gonal-f®	722:729	Increasing concentrations of Gonal-f® or biosimilar (1 × 10-3-1 × 103 ng/ml) were used for treating human primary granulosa lutein cells (hGLC) and FSH receptor (FSHR)-transfected HEK293 cells in vitro.
31396162	3	113	theme	alfa	388:391	arg1	responses					475:483	the originator (reference) follitropin alfa (Gonal-f®)- with biosimilar preparations (Bemfola® and Ovaleap®)-induced cellular responses	349:483	the originator (reference) follitropin alfa (Gonal-f®)- with biosimilar preparations (Bemfola® and Ovaleap®)-induced cellular responses	349:483	The aim of the study is to compare the originator (reference) follitropin alfa (Gonal-f®)- with biosimilar preparations (Bemfola® and Ovaleap®)-induced cellular responses in vitro.
31396162	8	114	theme	β-arrestin	1429:1438	arg1	range					1447:1451	β-arrestin 2 EC50 range	1429:1451	β-arrestin 2 EC50 range	1429:1451	We found preparation-specific glycosylation patterns by lectin assay (Kruskal-Wallis test; p < 0.001; n = 6), and similar cAMP production and β-arrestin 2 recruitment in FSHR-transfected HEK293 cells (cAMP EC50 range = 12 ± 0.9-24 ± 1.7 ng/ml; β-arrestin 2 EC50 range = 140 ± 14.1-313 ± 18.7 ng/ml; Kruskal-Wallis test; p ≥ 0.05; n = 4).
31396162	3	115	theme	cellular	466:473	arg1	responses					475:483	the originator (reference) follitropin alfa (Gonal-f®)- with biosimilar preparations (Bemfola® and Ovaleap®)-induced cellular responses	349:483	the originator (reference) follitropin alfa (Gonal-f®)- with biosimilar preparations (Bemfola® and Ovaleap®)-induced cellular responses	349:483	The aim of the study is to compare the originator (reference) follitropin alfa (Gonal-f®)- with biosimilar preparations (Bemfola® and Ovaleap®)-induced cellular responses in vitro.
31396162	0	116	dep	in	26:27	arg1	vitro					29:33	vitro	29:33	vitro	29:33	Glycosylation Pattern and in vitro Bioactivity of Reference Follitropin alfa and Biosimilars.
31396162	11	117	theme	overall	2125:2131	arg1	homogeneity					2144:2154	overall structural homogeneity	2125:2154	overall structural homogeneity	2125:2154	Apart from preparation-specific intracellular Ca2+ increases achieved at supra-physiological hormone doses, all compounds induced similar intracellular responses and steroidogenesis, reflecting similar bioactivity, and overall structural homogeneity.
31396162	8	118	theme	cAMP	1307:1310	arg1	production					1312:1321	similar cAMP production	1299:1321	similar cAMP production	1299:1321	We found preparation-specific glycosylation patterns by lectin assay (Kruskal-Wallis test; p < 0.001; n = 6), and similar cAMP production and β-arrestin 2 recruitment in FSHR-transfected HEK293 cells (cAMP EC50 range = 12 ± 0.9-24 ± 1.7 ng/ml; β-arrestin 2 EC50 range = 140 ± 14.1-313 ± 18.7 ng/ml; Kruskal-Wallis test; p ≥ 0.05; n = 4).
31396162	2	119	contain	have	225:228	arg1	They					220:223	They	220:223	They	220:223	They have specific FSH activity and a unique glycosylation profile dependent on source cells.
31396162	2	119	contain	have	225:228	arg2	activity					243:250	specific FSH activity	230:250	specific FSH activity	230:250	They have specific FSH activity and a unique glycosylation profile dependent on source cells.
31396162	2	119	contain	have	225:228	arg2	profile					279:285	a unique glycosylation profile	256:285	a unique glycosylation profile dependent on source cells	256:311	They have specific FSH activity and a unique glycosylation profile dependent on source cells.
31396162	4	120	theme	Gonadotropin	495:506	arg1	profiles					524:531	Gonadotropin N-glycosylation profiles	495:531	Gonadotropin N-glycosylation profiles	495:531	Gonadotropin N-glycosylation profiles were analyzed by ELISA lectin assay, revealing preparation specific-patterns of glycan species (Kruskal-Wallis test; p < 0.05, n = 6) and by glycotope mapping.
31396162	10	121	theme	different	1832:1840	arg1	EC50s					1842:1846	no different EC50s	1829:1846	no different EC50s	1829:1846	All preparations induced both 8 and 24 h-progesterone and estradiol synthesis in hGLC, while no different EC50s were demonstrated (Kruskal-Wallis test; p > 0.05; n = 5).
31396162	4	122	theme	species	620:626	arg1	specific-patterns					592:608	preparation specific-patterns	580:608	preparation specific-patterns of glycan species (Kruskal-Wallis test; p < 0.05, n = 6) and by glycotope mapping	580:690	Gonadotropin N-glycosylation profiles were analyzed by ELISA lectin assay, revealing preparation specific-patterns of glycan species (Kruskal-Wallis test; p < 0.05, n = 6) and by glycotope mapping.
31396162	4	123	theme	glycan	613:618	arg1	species					620:626	glycan species	613:626	glycan species (Kruskal-Wallis test; p < 0.05, n = 6)	613:665	Gonadotropin N-glycosylation profiles were analyzed by ELISA lectin assay, revealing preparation specific-patterns of glycan species (Kruskal-Wallis test; p < 0.05, n = 6) and by glycotope mapping.
30249003	3	0	theme	physiological	502:514	arg1	adaptions					516:524	physiological adaptions	502:524	physiological adaptions	502:524	Although the vast majority of prior studies emphasize the efficacy of aerobic exercise in promoting physiological adaptions, other types of exercise, such as resistance exercise and high-intensity interval training (HIIT), may achieve similar or different physiological outcomes.
30249003	11	1	from	exercise	1764:1771	arg1	combination					1776:1786	combination	1776:1786	combination with resveratrol supplementation	1776:1819	Resistance exercise in combination with resveratrol supplementation may be applied in the general population to achieve better physiological benefits, promote overall health, and promote participation in regular physical activities.
30249003	9	2	theme	aerobic	1593:1599	arg1	capacity					1601:1608	elevating aerobic capacity	1583:1608	elevating aerobic capacity	1583:1608	Moreover, the training or RES may have contributed significantly to elevating aerobic capacity, tissue glycogen, and muscle hypertrophy.
30249003	0	3	theme	Exercise	75:82	arg1	Performance					84:94	Exercise Performance	75:94	Exercise Performance	75:94	The Synergistic Effects of Resveratrol combined with Resistant Training on Exercise Performance and Physiological Adaption.
30249003	3	4	theme	different	648:656	arg1	outcomes					672:679	similar or different physiological outcomes	637:679	similar or different physiological outcomes	637:679	Although the vast majority of prior studies emphasize the efficacy of aerobic exercise in promoting physiological adaptions, other types of exercise, such as resistance exercise and high-intensity interval training (HIIT), may achieve similar or different physiological outcomes.
30249003	7	5	theme	muscular	1249:1256	arg1	characteristics					1258:1272	muscular characteristics	1249:1272	muscular characteristics	1249:1272	In addition, glycogen levels, muscular characteristics, and safety issues, including body composition, histopathology, and biochemistry, were further elucidated.
30249003	7	5	theme	muscular	1249:1256	arg1	composition					1309:1319	body composition	1304:1319	body composition	1304:1319	In addition, glycogen levels, muscular characteristics, and safety issues, including body composition, histopathology, and biochemistry, were further elucidated.
30249003	7	5	theme	muscular	1249:1256	arg1	histopathology					1322:1335	histopathology	1322:1335	histopathology	1322:1335	In addition, glycogen levels, muscular characteristics, and safety issues, including body composition, histopathology, and biochemistry, were further elucidated.
30249003	7	5	theme	muscular	1249:1256	arg1	biochemistry					1342:1353	biochemistry	1342:1353	biochemistry	1342:1353	In addition, glycogen levels, muscular characteristics, and safety issues, including body composition, histopathology, and biochemistry, were further elucidated.
30249003	1	6	theme	dietary	259:265	arg1	supplementation					267:281	dietary supplementation	259:281	dietary supplementation	259:281	The comprehensive studies done on resveratrol (RES) support that this polyphenol has multiple bioactivities and is widely accepted for dietary supplementation.
30249003	0	7	theme	Physiological	100:112	arg1	Adaption					114:121	Physiological Adaption	100:121	Physiological Adaption	100:121	The Synergistic Effects of Resveratrol combined with Resistant Training on Exercise Performance and Physiological Adaption.
30249003	11	8	theme	regular	1957:1963	arg1	activities					1974:1983	regular physical activities	1957:1983	regular physical activities	1957:1983	Resistance exercise in combination with resveratrol supplementation may be applied in the general population to achieve better physiological benefits, promote overall health, and promote participation in regular physical activities.
30249003	3	9	theme	exercise	542:549	arg1	types					533:537	other types	527:537	other types	527:537	Although the vast majority of prior studies emphasize the efficacy of aerobic exercise in promoting physiological adaptions, other types of exercise, such as resistance exercise and high-intensity interval training (HIIT), may achieve similar or different physiological outcomes.
30249003	3	9	theme	exercise	542:549	arg1	training					608:615	high-intensity interval training	584:615	high-intensity interval training (HIIT)	584:622	Although the vast majority of prior studies emphasize the efficacy of aerobic exercise in promoting physiological adaptions, other types of exercise, such as resistance exercise and high-intensity interval training (HIIT), may achieve similar or different physiological outcomes.
30249003	3	9	theme	exercise	542:549	arg1	exercise					571:578	resistance exercise	560:578	resistance exercise	560:578	Although the vast majority of prior studies emphasize the efficacy of aerobic exercise in promoting physiological adaptions, other types of exercise, such as resistance exercise and high-intensity interval training (HIIT), may achieve similar or different physiological outcomes.
30249003	8	10	located	observed	1406:1413	arg1	lactate					1468:1474	exercise lactate	1459:1474	exercise lactate	1459:1474	Synergistic effects were observed on grip strength, anaerobic capacities, and exercise lactate, with significant interaction effects.
30249003	8	10	located	observed	1406:1413	arg2	effects					1393:1399	Synergistic effects	1381:1399	Synergistic effects	1381:1399	Synergistic effects were observed on grip strength, anaerobic capacities, and exercise lactate, with significant interaction effects.
30249003	8	10	located	observed	1406:1413	arg1	strength					1423:1430	grip strength	1418:1430	grip strength	1418:1430	Synergistic effects were observed on grip strength, anaerobic capacities, and exercise lactate, with significant interaction effects.
30249003	8	10	located	observed	1406:1413	arg1	capacities					1443:1452	anaerobic capacities	1433:1452	anaerobic capacities	1433:1452	Synergistic effects were observed on grip strength, anaerobic capacities, and exercise lactate, with significant interaction effects.
30249003	6	11	theme	Exercise	964:971	arg1	capacities					973:982	Exercise capacities	964:982	Exercise capacities	964:982	Exercise capacities, including grip strength, aerobic performance, and anaerobic performance, were assessed and the physiological adaptions were evaluated using fatigue-associated indexes that were implemented immediately after the exercise intervention.
30249003	6	11	theme	Exercise	964:971	arg1	performance					1045:1055	anaerobic performance	1035:1055	anaerobic performance	1035:1055	Exercise capacities, including grip strength, aerobic performance, and anaerobic performance, were assessed and the physiological adaptions were evaluated using fatigue-associated indexes that were implemented immediately after the exercise intervention.
30249003	6	11	theme	Exercise	964:971	arg1	strength					1000:1007	grip strength	995:1007	grip strength	995:1007	Exercise capacities, including grip strength, aerobic performance, and anaerobic performance, were assessed and the physiological adaptions were evaluated using fatigue-associated indexes that were implemented immediately after the exercise intervention.
30249003	6	11	theme	Exercise	964:971	arg1	performance					1018:1028	aerobic performance	1010:1028	aerobic performance	1010:1028	Exercise capacities, including grip strength, aerobic performance, and anaerobic performance, were assessed and the physiological adaptions were evaluated using fatigue-associated indexes that were implemented immediately after the exercise intervention.
30249003	4	12	theme	combinational	734:746	arg1	approach					761:768	a combinational, synergistic approach	732:768	a combinational, synergistic approach to exercise	732:780	Few studies have looked into the effectiveness of a combinational, synergistic approach to exercise using a weight-loading ladder climbing animal platform.
30249003	4	13	theme	approach	761:768	arg1	effectiveness					715:727	the effectiveness	711:727	the effectiveness of a combinational, synergistic approach to exercise	711:780	Few studies have looked into the effectiveness of a combinational, synergistic approach to exercise using a weight-loading ladder climbing animal platform.
30249003	11	14	theme	Resistance	1753:1762	arg1	exercise					1764:1771	Resistance exercise	1753:1771	Resistance exercise in combination with resveratrol supplementation	1753:1819	Resistance exercise in combination with resveratrol supplementation may be applied in the general population to achieve better physiological benefits, promote overall health, and promote participation in regular physical activities.
30249003	2	15	theme	regular	297:303	arg1	form					373:376	a form	371:376	a form of preventive medicine	371:399	Furthermore, regular exercise is known to have benefits on health and is considered as a form of preventive medicine.
30249003	2	15	theme	regular	297:303	arg1	exercise					305:312	regular exercise	297:312	regular exercise	297:312	Furthermore, regular exercise is known to have benefits on health and is considered as a form of preventive medicine.
30249003	7	16	theme	safety	1279:1284	arg1	composition					1309:1319	body composition	1304:1319	body composition	1304:1319	In addition, glycogen levels, muscular characteristics, and safety issues, including body composition, histopathology, and biochemistry, were further elucidated.
30249003	7	16	theme	safety	1279:1284	arg1	issues					1286:1291	safety issues	1279:1291	safety issues	1279:1291	In addition, glycogen levels, muscular characteristics, and safety issues, including body composition, histopathology, and biochemistry, were further elucidated.
30249003	7	16	theme	safety	1279:1284	arg1	histopathology					1322:1335	histopathology	1322:1335	histopathology	1322:1335	In addition, glycogen levels, muscular characteristics, and safety issues, including body composition, histopathology, and biochemistry, were further elucidated.
30249003	7	16	theme	safety	1279:1284	arg1	biochemistry					1342:1353	biochemistry	1342:1353	biochemistry	1342:1353	In addition, glycogen levels, muscular characteristics, and safety issues, including body composition, histopathology, and biochemistry, were further elucidated.
30249003	10	17	theme	deleterious	1668:1678	arg1	effects					1680:1686	Toxic and other deleterious effects	1652:1686	Toxic and other deleterious effects	1652:1686	Toxic and other deleterious effects were also considered to evaluate the safety of the intervention.
30249003	2	18	theme	medicine	392:399	arg1	form					373:376	a form	371:376	a form of preventive medicine	371:399	Furthermore, regular exercise is known to have benefits on health and is considered as a form of preventive medicine.
30249003	2	18	theme	medicine	392:399	arg1	exercise					305:312	regular exercise	297:312	regular exercise	297:312	Furthermore, regular exercise is known to have benefits on health and is considered as a form of preventive medicine.
30249003	10	19	theme	Toxic	1652:1656	arg1	effects					1680:1686	Toxic and other deleterious effects	1652:1686	Toxic and other deleterious effects	1652:1686	Toxic and other deleterious effects were also considered to evaluate the safety of the intervention.
30249003	5	20	theme	two-way	925:931	arg1	Training					946:953	Training	946:953	Training	946:953	In this study, ICR mice were allocated randomly to the RES and training groups using a two-way ANOVA (RES × Training) design.
30249003	5	20	theme	two-way	925:931	arg1	ANOVA					933:937	a two-way ANOVA	923:937	a two-way ANOVA (RES × Training) design	923:961	In this study, ICR mice were allocated randomly to the RES and training groups using a two-way ANOVA (RES × Training) design.
30249003	3	21	theme	studies	438:444	arg1	majority					420:427	the vast majority	411:427	the vast majority of prior studies	411:444	Although the vast majority of prior studies emphasize the efficacy of aerobic exercise in promoting physiological adaptions, other types of exercise, such as resistance exercise and high-intensity interval training (HIIT), may achieve similar or different physiological outcomes.
30249003	0	22	theme	Synergistic	4:14	arg1	Effects					16:22	The Synergistic Effects	0:22	The Synergistic Effects of Resveratrol	0:37	The Synergistic Effects of Resveratrol combined with Resistant Training on Exercise Performance and Physiological Adaption.
30249003	11	23	theme	physiological	1880:1892	arg1	benefits					1894:1901	better physiological benefits	1873:1901	better physiological benefits	1873:1901	Resistance exercise in combination with resveratrol supplementation may be applied in the general population to achieve better physiological benefits, promote overall health, and promote participation in regular physical activities.
30249003	3	24	theme	resistance	560:569	arg1	exercise					571:578	resistance exercise	560:578	resistance exercise	560:578	Although the vast majority of prior studies emphasize the efficacy of aerobic exercise in promoting physiological adaptions, other types of exercise, such as resistance exercise and high-intensity interval training (HIIT), may achieve similar or different physiological outcomes.
30249003	10	25	theme	other	1662:1666	arg1	effects					1680:1686	Toxic and other deleterious effects	1652:1686	Toxic and other deleterious effects	1652:1686	Toxic and other deleterious effects were also considered to evaluate the safety of the intervention.
30249003	8	26	theme	interaction	1494:1504	arg1	effects					1506:1512	significant interaction effects	1482:1512	significant interaction effects	1482:1512	Synergistic effects were observed on grip strength, anaerobic capacities, and exercise lactate, with significant interaction effects.
30249003	5	27	theme	ANOVA	933:937	arg1	design					956:961	a two-way ANOVA (RES × Training) design	923:961	a two-way ANOVA (RES × Training) design	923:961	In this study, ICR mice were allocated randomly to the RES and training groups using a two-way ANOVA (RES × Training) design.
30249003	6	28	theme	anaerobic	1035:1043	arg1	performance					1045:1055	anaerobic performance	1035:1055	anaerobic performance	1035:1055	Exercise capacities, including grip strength, aerobic performance, and anaerobic performance, were assessed and the physiological adaptions were evaluated using fatigue-associated indexes that were implemented immediately after the exercise intervention.
30249003	11	29	theme	general	1843:1849	arg1	population					1851:1860	the general population	1839:1860	the general population	1839:1860	Resistance exercise in combination with resveratrol supplementation may be applied in the general population to achieve better physiological benefits, promote overall health, and promote participation in regular physical activities.
30249003	8	30	theme	grip	1418:1421	arg1	strength					1423:1430	grip strength	1418:1430	grip strength	1418:1430	Synergistic effects were observed on grip strength, anaerobic capacities, and exercise lactate, with significant interaction effects.
30249003	9	31	theme	tissue	1611:1616	arg1	glycogen					1618:1625	tissue glycogen	1611:1625	tissue glycogen	1611:1625	Moreover, the training or RES may have contributed significantly to elevating aerobic capacity, tissue glycogen, and muscle hypertrophy.
30249003	9	32	theme	muscle	1632:1637	arg1	hypertrophy					1639:1649	muscle hypertrophy	1632:1649	muscle hypertrophy	1632:1649	Moreover, the training or RES may have contributed significantly to elevating aerobic capacity, tissue glycogen, and muscle hypertrophy.
30249003	5	33	dep	RES	893:895	arg1	the					889:891	the	889:891	the	889:891	In this study, ICR mice were allocated randomly to the RES and training groups using a two-way ANOVA (RES × Training) design.
30249003	3	34	theme	similar	637:643	arg1	outcomes					672:679	similar or different physiological outcomes	637:679	similar or different physiological outcomes	637:679	Although the vast majority of prior studies emphasize the efficacy of aerobic exercise in promoting physiological adaptions, other types of exercise, such as resistance exercise and high-intensity interval training (HIIT), may achieve similar or different physiological outcomes.
30249003	6	35	theme	fatigue-associated	1125:1142	arg1	indexes					1144:1150	fatigue-associated indexes	1125:1150	fatigue-associated indexes that were implemented immediately after the exercise intervention	1125:1216	Exercise capacities, including grip strength, aerobic performance, and anaerobic performance, were assessed and the physiological adaptions were evaluated using fatigue-associated indexes that were implemented immediately after the exercise intervention.
30249003	8	36	theme	Synergistic	1381:1391	arg1	effects					1393:1399	Synergistic effects	1381:1399	Synergistic effects	1381:1399	Synergistic effects were observed on grip strength, anaerobic capacities, and exercise lactate, with significant interaction effects.
30249003	7	37	theme	body	1304:1307	arg1	composition					1309:1319	body composition	1304:1319	body composition	1304:1319	In addition, glycogen levels, muscular characteristics, and safety issues, including body composition, histopathology, and biochemistry, were further elucidated.
30249003	9	38	theme	elevating	1583:1591	arg1	capacity					1601:1608	elevating aerobic capacity	1583:1608	elevating aerobic capacity	1583:1608	Moreover, the training or RES may have contributed significantly to elevating aerobic capacity, tissue glycogen, and muscle hypertrophy.
30249003	11	39	theme	physical	1965:1972	arg1	activities					1974:1983	regular physical activities	1957:1983	regular physical activities	1957:1983	Resistance exercise in combination with resveratrol supplementation may be applied in the general population to achieve better physiological benefits, promote overall health, and promote participation in regular physical activities.
30249003	3	40	theme	interval	599:606	arg1	HIIT					618:621	HIIT	618:621	HIIT	618:621	Although the vast majority of prior studies emphasize the efficacy of aerobic exercise in promoting physiological adaptions, other types of exercise, such as resistance exercise and high-intensity interval training (HIIT), may achieve similar or different physiological outcomes.
30249003	3	40	theme	interval	599:606	arg1	training					608:615	high-intensity interval training	584:615	high-intensity interval training (HIIT)	584:622	Although the vast majority of prior studies emphasize the efficacy of aerobic exercise in promoting physiological adaptions, other types of exercise, such as resistance exercise and high-intensity interval training (HIIT), may achieve similar or different physiological outcomes.
30249003	6	41	theme	exercise	1196:1203	arg1	intervention					1205:1216	the exercise intervention	1192:1216	the exercise intervention	1192:1216	Exercise capacities, including grip strength, aerobic performance, and anaerobic performance, were assessed and the physiological adaptions were evaluated using fatigue-associated indexes that were implemented immediately after the exercise intervention.
30249003	1	42	theme	multiple	209:216	arg1	bioactivities					218:230	multiple bioactivities	209:230	multiple bioactivities	209:230	The comprehensive studies done on resveratrol (RES) support that this polyphenol has multiple bioactivities and is widely accepted for dietary supplementation.
30249003	0	43	theme	Resveratrol	27:37	arg1	Effects					16:22	The Synergistic Effects	0:22	The Synergistic Effects of Resveratrol	0:37	The Synergistic Effects of Resveratrol combined with Resistant Training on Exercise Performance and Physiological Adaption.
30249003	11	44	theme	overall	1912:1918	arg1	health					1920:1925	overall health	1912:1925	overall health	1912:1925	Resistance exercise in combination with resveratrol supplementation may be applied in the general population to achieve better physiological benefits, promote overall health, and promote participation in regular physical activities.
30249003	3	45	theme	vast	415:418	arg1	majority					420:427	the vast majority	411:427	the vast majority of prior studies	411:444	Although the vast majority of prior studies emphasize the efficacy of aerobic exercise in promoting physiological adaptions, other types of exercise, such as resistance exercise and high-intensity interval training (HIIT), may achieve similar or different physiological outcomes.
30249003	4	46	theme	animal	821:826	arg1	platform					828:835	a weight-loading ladder climbing animal platform	788:835	a weight-loading ladder climbing animal platform	788:835	Few studies have looked into the effectiveness of a combinational, synergistic approach to exercise using a weight-loading ladder climbing animal platform.
30249003	8	47	theme	exercise	1459:1466	arg1	lactate					1468:1474	exercise lactate	1459:1474	exercise lactate	1459:1474	Synergistic effects were observed on grip strength, anaerobic capacities, and exercise lactate, with significant interaction effects.
30249003	5	48	theme	ICR	853:855	arg1	mice					857:860	ICR mice	853:860	ICR mice	853:860	In this study, ICR mice were allocated randomly to the RES and training groups using a two-way ANOVA (RES × Training) design.
30249003	0	49	theme	Resistant	53:61	arg1	Training					63:70	Resistant Training	53:70	Resistant Training	53:70	The Synergistic Effects of Resveratrol combined with Resistant Training on Exercise Performance and Physiological Adaption.
30249003	11	50	theme	better	1873:1878	arg1	benefits					1894:1901	better physiological benefits	1873:1901	better physiological benefits	1873:1901	Resistance exercise in combination with resveratrol supplementation may be applied in the general population to achieve better physiological benefits, promote overall health, and promote participation in regular physical activities.
30249003	6	51	theme	aerobic	1010:1016	arg1	performance					1018:1028	aerobic performance	1010:1028	aerobic performance	1010:1028	Exercise capacities, including grip strength, aerobic performance, and anaerobic performance, were assessed and the physiological adaptions were evaluated using fatigue-associated indexes that were implemented immediately after the exercise intervention.
30249003	3	52	theme	prior	432:436	arg1	studies					438:444	prior studies	432:444	prior studies	432:444	Although the vast majority of prior studies emphasize the efficacy of aerobic exercise in promoting physiological adaptions, other types of exercise, such as resistance exercise and high-intensity interval training (HIIT), may achieve similar or different physiological outcomes.
30249003	5	53	theme	training	901:908	arg1	groups					910:915	training groups	901:915	training groups	901:915	In this study, ICR mice were allocated randomly to the RES and training groups using a two-way ANOVA (RES × Training) design.
30249003	8	54	theme	significant	1482:1492	arg1	effects					1506:1512	significant interaction effects	1482:1512	significant interaction effects	1482:1512	Synergistic effects were observed on grip strength, anaerobic capacities, and exercise lactate, with significant interaction effects.
30249003	3	55	theme	high-intensity	584:597	arg1	HIIT					618:621	HIIT	618:621	HIIT	618:621	Although the vast majority of prior studies emphasize the efficacy of aerobic exercise in promoting physiological adaptions, other types of exercise, such as resistance exercise and high-intensity interval training (HIIT), may achieve similar or different physiological outcomes.
30249003	3	55	theme	high-intensity	584:597	arg1	training					608:615	high-intensity interval training	584:615	high-intensity interval training (HIIT)	584:622	Although the vast majority of prior studies emphasize the efficacy of aerobic exercise in promoting physiological adaptions, other types of exercise, such as resistance exercise and high-intensity interval training (HIIT), may achieve similar or different physiological outcomes.
30249003	1	56	theme	comprehensive	128:140	arg1	studies					142:148	The comprehensive studies	124:148	The comprehensive studies done on resveratrol (RES)	124:174	The comprehensive studies done on resveratrol (RES) support that this polyphenol has multiple bioactivities and is widely accepted for dietary supplementation.
30249003	2	57	contain	have	326:329	arg2	benefits					331:338	benefits	331:338	benefits	331:338	Furthermore, regular exercise is known to have benefits on health and is considered as a form of preventive medicine.
30249003	2	57	contain	have	326:329	arg1	form					373:376	a form	371:376	a form of preventive medicine	371:399	Furthermore, regular exercise is known to have benefits on health and is considered as a form of preventive medicine.
30249003	2	57	contain	have	326:329	arg1	exercise					305:312	regular exercise	297:312	regular exercise	297:312	Furthermore, regular exercise is known to have benefits on health and is considered as a form of preventive medicine.
30249003	8	58	theme	anaerobic	1433:1441	arg1	capacities					1443:1452	anaerobic capacities	1433:1452	anaerobic capacities	1433:1452	Synergistic effects were observed on grip strength, anaerobic capacities, and exercise lactate, with significant interaction effects.
30249003	4	59	theme	weight-loading	790:803	arg1	platform					828:835	a weight-loading ladder climbing animal platform	788:835	a weight-loading ladder climbing animal platform	788:835	Few studies have looked into the effectiveness of a combinational, synergistic approach to exercise using a weight-loading ladder climbing animal platform.
30249003	4	60	theme	Few	682:684	arg1	studies					686:692	Few studies	682:692	Few studies	682:692	Few studies have looked into the effectiveness of a combinational, synergistic approach to exercise using a weight-loading ladder climbing animal platform.
30249003	3	61	theme	aerobic	472:478	arg1	exercise					480:487	aerobic exercise	472:487	aerobic exercise	472:487	Although the vast majority of prior studies emphasize the efficacy of aerobic exercise in promoting physiological adaptions, other types of exercise, such as resistance exercise and high-intensity interval training (HIIT), may achieve similar or different physiological outcomes.
30249003	10	62	theme	intervention	1739:1750	arg1	safety					1725:1730	the safety	1721:1730	the safety of the intervention	1721:1750	Toxic and other deleterious effects were also considered to evaluate the safety of the intervention.
30249003	1	63	contain	has	205:207	arg2	bioactivities					218:230	multiple bioactivities	209:230	multiple bioactivities	209:230	The comprehensive studies done on resveratrol (RES) support that this polyphenol has multiple bioactivities and is widely accepted for dietary supplementation.
30249003	1	63	contain	has	205:207	arg1	polyphenol					194:203	this polyphenol	189:203	this polyphenol	189:203	The comprehensive studies done on resveratrol (RES) support that this polyphenol has multiple bioactivities and is widely accepted for dietary supplementation.
30249003	3	64	theme	exercise	480:487	arg1	efficacy					460:467	the efficacy	456:467	the efficacy of aerobic exercise in promoting physiological adaptions	456:524	Although the vast majority of prior studies emphasize the efficacy of aerobic exercise in promoting physiological adaptions, other types of exercise, such as resistance exercise and high-intensity interval training (HIIT), may achieve similar or different physiological outcomes.
30249003	4	65	dep	combinational	734:746	arg1	synergistic					749:759	synergistic	749:759	synergistic	749:759	Few studies have looked into the effectiveness of a combinational, synergistic approach to exercise using a weight-loading ladder climbing animal platform.
30249003	7	66	theme	glycogen	1232:1239	arg1	composition					1309:1319	body composition	1304:1319	body composition	1304:1319	In addition, glycogen levels, muscular characteristics, and safety issues, including body composition, histopathology, and biochemistry, were further elucidated.
30249003	7	66	theme	glycogen	1232:1239	arg1	biochemistry					1342:1353	biochemistry	1342:1353	biochemistry	1342:1353	In addition, glycogen levels, muscular characteristics, and safety issues, including body composition, histopathology, and biochemistry, were further elucidated.
30249003	7	66	theme	glycogen	1232:1239	arg1	histopathology					1322:1335	histopathology	1322:1335	histopathology	1322:1335	In addition, glycogen levels, muscular characteristics, and safety issues, including body composition, histopathology, and biochemistry, were further elucidated.
30249003	7	66	theme	glycogen	1232:1239	arg1	levels					1241:1246	glycogen levels	1232:1246	glycogen levels	1232:1246	In addition, glycogen levels, muscular characteristics, and safety issues, including body composition, histopathology, and biochemistry, were further elucidated.
30249003	3	67	theme	physiological	658:670	arg1	outcomes					672:679	similar or different physiological outcomes	637:679	similar or different physiological outcomes	637:679	Although the vast majority of prior studies emphasize the efficacy of aerobic exercise in promoting physiological adaptions, other types of exercise, such as resistance exercise and high-intensity interval training (HIIT), may achieve similar or different physiological outcomes.
30249003	4	68	theme	climbing	812:819	arg1	platform					828:835	a weight-loading ladder climbing animal platform	788:835	a weight-loading ladder climbing animal platform	788:835	Few studies have looked into the effectiveness of a combinational, synergistic approach to exercise using a weight-loading ladder climbing animal platform.
30249003	11	69	from	participation	1940:1952	arg1	activities					1974:1983	regular physical activities	1957:1983	regular physical activities	1957:1983	Resistance exercise in combination with resveratrol supplementation may be applied in the general population to achieve better physiological benefits, promote overall health, and promote participation in regular physical activities.
30249003	6	70	theme	physiological	1080:1092	arg1	adaptions					1094:1102	the physiological adaptions	1076:1102	the physiological adaptions	1076:1102	Exercise capacities, including grip strength, aerobic performance, and anaerobic performance, were assessed and the physiological adaptions were evaluated using fatigue-associated indexes that were implemented immediately after the exercise intervention.
30249003	2	71	theme	preventive	381:390	arg1	medicine					392:399	preventive medicine	381:399	preventive medicine	381:399	Furthermore, regular exercise is known to have benefits on health and is considered as a form of preventive medicine.
30249003	6	72	theme	grip	995:998	arg1	strength					1000:1007	grip strength	995:1007	grip strength	995:1007	Exercise capacities, including grip strength, aerobic performance, and anaerobic performance, were assessed and the physiological adaptions were evaluated using fatigue-associated indexes that were implemented immediately after the exercise intervention.
30249003	11	73	theme	resveratrol	1793:1803	arg1	supplementation					1805:1819	resveratrol supplementation	1793:1819	resveratrol supplementation	1793:1819	Resistance exercise in combination with resveratrol supplementation may be applied in the general population to achieve better physiological benefits, promote overall health, and promote participation in regular physical activities.
30249003	4	74	theme	ladder	805:810	arg1	platform					828:835	a weight-loading ladder climbing animal platform	788:835	a weight-loading ladder climbing animal platform	788:835	Few studies have looked into the effectiveness of a combinational, synergistic approach to exercise using a weight-loading ladder climbing animal platform.
30249003	11	75	with	combination	1776:1786	arg1	supplementation					1805:1819	resveratrol supplementation	1793:1819	resveratrol supplementation	1793:1819	Resistance exercise in combination with resveratrol supplementation may be applied in the general population to achieve better physiological benefits, promote overall health, and promote participation in regular physical activities.
30249003	3	76	theme	other	527:531	arg1	types					533:537	other types	527:537	other types	527:537	Although the vast majority of prior studies emphasize the efficacy of aerobic exercise in promoting physiological adaptions, other types of exercise, such as resistance exercise and high-intensity interval training (HIIT), may achieve similar or different physiological outcomes.
30249003	3	76	theme	other	527:531	arg1	training					608:615	high-intensity interval training	584:615	high-intensity interval training (HIIT)	584:622	Although the vast majority of prior studies emphasize the efficacy of aerobic exercise in promoting physiological adaptions, other types of exercise, such as resistance exercise and high-intensity interval training (HIIT), may achieve similar or different physiological outcomes.
30249003	3	76	theme	other	527:531	arg1	exercise					571:578	resistance exercise	560:578	resistance exercise	560:578	Although the vast majority of prior studies emphasize the efficacy of aerobic exercise in promoting physiological adaptions, other types of exercise, such as resistance exercise and high-intensity interval training (HIIT), may achieve similar or different physiological outcomes.
30137571	5	0	from	changes	957:963	arg1	microflora					974:983	cecal microflora	968:983	cecal microflora of broilers fed with PKE diets	968:1014	At present, little is known about the changes on cecal microflora of broilers fed with PKE diets.
30137571	9	1	theme	OligoPKE	1688:1695	arg1	supplementation					1669:1683	supplementation	1669:1683	supplementation of OligoPKE	1669:1695	In addition, supplementation of OligoPKE increased (P < 0.05) the relative abundance of Lactobacillus on both D14 and D28, signifying its effect as prebiotics in enhancing growth of indigenous Lactobacillus.
30137571	1	2	theme	fiber	237:241	arg1	content					243:249	the high fiber content	228:249	the high fiber content	228:249	The potential use of palm kernel expeller (PKE) as an alternative energy source in broiler diets is limited by the high fiber content.
30137571	6	3	theme	different	1081:1089	arg1	forms					1091:1095	different forms	1081:1095	different forms of PKE	1081:1102	Hence, this study was designed to assess the effects of feeding different forms of PKE; namely untreated PKE (UPKE), enzyme-treated PKE (EPKE), and oligosaccharides extracted from PKE (OligoPKE), on the cecal microbiota of broiler chickens at 14 d old (day 14) and 28 d old (day 28) using 16S rRNA gene high-throughput next-generation sequencing method.
30137571	3	4	theme	cecal	714:718	arg1	microflora					720:729	the cecal microflora	710:729	the cecal microflora	710:729	As chicken's ceca are the most heavily populated with microflora within their gastrointestinal tract, it was hypothesized that any modulation of the intestinal environment by dietary treatments should be reflected by the composition and activities of the cecal microflora.
30137571	6	5	theme	sequencing	1352:1361	arg1	method					1363:1368	16S rRNA gene high-throughput next-generation sequencing method	1306:1368	16S rRNA gene high-throughput next-generation sequencing method	1306:1368	Hence, this study was designed to assess the effects of feeding different forms of PKE; namely untreated PKE (UPKE), enzyme-treated PKE (EPKE), and oligosaccharides extracted from PKE (OligoPKE), on the cecal microbiota of broiler chickens at 14 d old (day 14) and 28 d old (day 28) using 16S rRNA gene high-throughput next-generation sequencing method.
30137571	10	6	theme	metabolizable	2103:2115	arg1	content					2129:2135	higher metabolizable energy (ME) content	2096:2135	higher metabolizable energy (ME) content	2096:2135	Our results showed that cecal microbiota was significantly modulated by dietary treatments and that the lower relative abundance of phylum Firmicutes in chickens fed with EPKE could be a reason why broiler chickens fed with EPKE of higher metabolizable energy (ME) content did not show improvement in their growth performance.
30137571	5	7	theme	PKE	1006:1008	arg1	diets					1010:1014	PKE diets	1006:1014	PKE diets	1006:1014	At present, little is known about the changes on cecal microflora of broilers fed with PKE diets.
30137571	6	8	theme	high-throughput	1320:1334	arg1	method					1363:1368	16S rRNA gene high-throughput next-generation sequencing method	1306:1368	16S rRNA gene high-throughput next-generation sequencing method	1306:1368	Hence, this study was designed to assess the effects of feeding different forms of PKE; namely untreated PKE (UPKE), enzyme-treated PKE (EPKE), and oligosaccharides extracted from PKE (OligoPKE), on the cecal microbiota of broiler chickens at 14 d old (day 14) and 28 d old (day 28) using 16S rRNA gene high-throughput next-generation sequencing method.
30137571	1	9	theme	energy	183:188	arg1	source					190:195	an alternative energy source	168:195	an alternative energy source in broiler diets	168:212	The potential use of palm kernel expeller (PKE) as an alternative energy source in broiler diets is limited by the high fiber content.
30137571	4	10	theme	improved	887:894	arg1	ratio					912:916	improved feed conversion ratio	887:916	improved feed conversion ratio	887:916	There is a correlation between cecal microbiota composition and the efficiency of the host to extract energy from the diet and to deposit that energy into improved feed conversion ratio.
30137571	6	11	theme	enzyme-treated	1134:1147	arg1	EPKE					1154:1157	EPKE	1154:1157	EPKE	1154:1157	Hence, this study was designed to assess the effects of feeding different forms of PKE; namely untreated PKE (UPKE), enzyme-treated PKE (EPKE), and oligosaccharides extracted from PKE (OligoPKE), on the cecal microbiota of broiler chickens at 14 d old (day 14) and 28 d old (day 28) using 16S rRNA gene high-throughput next-generation sequencing method.
30137571	6	11	theme	enzyme-treated	1134:1147	arg1	PKE					1149:1151	enzyme-treated PKE	1134:1151	enzyme-treated PKE (EPKE)	1134:1158	Hence, this study was designed to assess the effects of feeding different forms of PKE; namely untreated PKE (UPKE), enzyme-treated PKE (EPKE), and oligosaccharides extracted from PKE (OligoPKE), on the cecal microbiota of broiler chickens at 14 d old (day 14) and 28 d old (day 28) using 16S rRNA gene high-throughput next-generation sequencing method.
30137571	9	12	theme	relative	1722:1729	arg1	abundance					1731:1739	the relative abundance	1718:1739	the relative abundance of Lactobacillus	1718:1756	In addition, supplementation of OligoPKE increased (P < 0.05) the relative abundance of Lactobacillus on both D14 and D28, signifying its effect as prebiotics in enhancing growth of indigenous Lactobacillus.
30137571	6	13	from	d	1285:1285	arg1	microbiota					1226:1235	the cecal microbiota	1216:1235	the cecal microbiota of broiler chickens at 14 d old (day 14) and 28 d old (day 28)	1216:1298	Hence, this study was designed to assess the effects of feeding different forms of PKE; namely untreated PKE (UPKE), enzyme-treated PKE (EPKE), and oligosaccharides extracted from PKE (OligoPKE), on the cecal microbiota of broiler chickens at 14 d old (day 14) and 28 d old (day 28) using 16S rRNA gene high-throughput next-generation sequencing method.
30137571	1	14	from	source	190:195	arg1	diets					208:212	broiler diets	200:212	broiler diets	200:212	The potential use of palm kernel expeller (PKE) as an alternative energy source in broiler diets is limited by the high fiber content.
30137571	9	15	dep	increased	1697:1705	arg1	<					1710:1710	P < 0.05	1708:1715	P < 0.05	1708:1715	In addition, supplementation of OligoPKE increased (P < 0.05) the relative abundance of Lactobacillus on both D14 and D28, signifying its effect as prebiotics in enhancing growth of indigenous Lactobacillus.
30137571	1	16	theme	potential	121:129	arg1	use					131:133	The potential use	117:133	The potential use of palm kernel expeller (PKE) as an alternative energy source in broiler diets	117:212	The potential use of palm kernel expeller (PKE) as an alternative energy source in broiler diets is limited by the high fiber content.
30137571	7	17	theme	chickens	1443:1450	arg1	microbiota					1421:1430	cecal microbiota	1415:1430	cecal microbiota of broiler chickens	1415:1450	The results showed that temporal changes in cecal microbiota of broiler chickens were evident on day 14 and day 28.
30137571	10	18	from	improvement	2150:2160	arg1	performance					2178:2188	their growth performance	2165:2188	their growth performance	2165:2188	Our results showed that cecal microbiota was significantly modulated by dietary treatments and that the lower relative abundance of phylum Firmicutes in chickens fed with EPKE could be a reason why broiler chickens fed with EPKE of higher metabolizable energy (ME) content did not show improvement in their growth performance.
30137571	8	19	theme	age	1595:1597	arg1	groups					1599:1604	both age groups	1590:1604	both age groups	1590:1604	The relative abundance of phylum Firmicutes, known to be involved in nutrient uptake and absorption in both age groups was higher in the UPKE as compared to EPKE group.
30137571	6	20	theme	untreated	1112:1120	arg1	PKE					1122:1124	namely untreated PKE	1105:1124	namely untreated PKE (UPKE)	1105:1131	Hence, this study was designed to assess the effects of feeding different forms of PKE; namely untreated PKE (UPKE), enzyme-treated PKE (EPKE), and oligosaccharides extracted from PKE (OligoPKE), on the cecal microbiota of broiler chickens at 14 d old (day 14) and 28 d old (day 28) using 16S rRNA gene high-throughput next-generation sequencing method.
30137571	6	20	theme	untreated	1112:1120	arg1	UPKE					1127:1130	UPKE	1127:1130	UPKE	1127:1130	Hence, this study was designed to assess the effects of feeding different forms of PKE; namely untreated PKE (UPKE), enzyme-treated PKE (EPKE), and oligosaccharides extracted from PKE (OligoPKE), on the cecal microbiota of broiler chickens at 14 d old (day 14) and 28 d old (day 28) using 16S rRNA gene high-throughput next-generation sequencing method.
30137571	1	21	theme	kernel	143:148	arg1	PKE					160:162	PKE	160:162	PKE	160:162	The potential use of palm kernel expeller (PKE) as an alternative energy source in broiler diets is limited by the high fiber content.
30137571	1	21	theme	kernel	143:148	arg1	expeller					150:157	palm kernel expeller	138:157	palm kernel expeller (PKE)	138:163	The potential use of palm kernel expeller (PKE) as an alternative energy source in broiler diets is limited by the high fiber content.
30137571	7	22	theme	cecal	1415:1419	arg1	microbiota					1421:1430	cecal microbiota	1415:1430	cecal microbiota of broiler chickens	1415:1450	The results showed that temporal changes in cecal microbiota of broiler chickens were evident on day 14 and day 28.
30137571	10	23	theme	lower	1968:1972	arg1	reason					2051:2056	a reason why broiler chickens fed with EPKE of higher metabolizable energy (ME) content did not show improvement in their growth performance	2049:2188	a reason why broiler chickens fed with EPKE of higher metabolizable energy (ME) content did not show improvement in their growth performance	2049:2188	Our results showed that cecal microbiota was significantly modulated by dietary treatments and that the lower relative abundance of phylum Firmicutes in chickens fed with EPKE could be a reason why broiler chickens fed with EPKE of higher metabolizable energy (ME) content did not show improvement in their growth performance.
30137571	10	23	theme	lower	1968:1972	arg1	abundance					1983:1991	the lower relative abundance	1964:1991	the lower relative abundance of phylum Firmicutes in chickens fed with EPKE	1964:2038	Our results showed that cecal microbiota was significantly modulated by dietary treatments and that the lower relative abundance of phylum Firmicutes in chickens fed with EPKE could be a reason why broiler chickens fed with EPKE of higher metabolizable energy (ME) content did not show improvement in their growth performance.
30137571	6	24	theme	chickens	1248:1255	arg1	microbiota					1226:1235	the cecal microbiota	1216:1235	the cecal microbiota of broiler chickens at 14 d old (day 14) and 28 d old (day 28)	1216:1298	Hence, this study was designed to assess the effects of feeding different forms of PKE; namely untreated PKE (UPKE), enzyme-treated PKE (EPKE), and oligosaccharides extracted from PKE (OligoPKE), on the cecal microbiota of broiler chickens at 14 d old (day 14) and 28 d old (day 28) using 16S rRNA gene high-throughput next-generation sequencing method.
30137571	6	25	from	d	1263:1263	arg1	microbiota					1226:1235	the cecal microbiota	1216:1235	the cecal microbiota of broiler chickens at 14 d old (day 14) and 28 d old (day 28)	1216:1298	Hence, this study was designed to assess the effects of feeding different forms of PKE; namely untreated PKE (UPKE), enzyme-treated PKE (EPKE), and oligosaccharides extracted from PKE (OligoPKE), on the cecal microbiota of broiler chickens at 14 d old (day 14) and 28 d old (day 28) using 16S rRNA gene high-throughput next-generation sequencing method.
30137571	4	26	theme	conversion	901:910	arg1	ratio					912:916	improved feed conversion ratio	887:916	improved feed conversion ratio	887:916	There is a correlation between cecal microbiota composition and the efficiency of the host to extract energy from the diet and to deposit that energy into improved feed conversion ratio.
30137571	0	27	theme	kernel	94:99	arg1	diets					110:114	palm kernel expeller diets	89:114	palm kernel expeller diets	89:114	Metagenomics analysis reveals significant modulation of cecal microbiota of broilers fed palm kernel expeller diets.
30137571	2	28	theme	apparent	363:370	arg1	improvement					372:382	this apparent improvement	358:382	this apparent improvement	358:382	Although enzymatic treatment could alleviate the fiber component and increase the nutritive value of PKE, this apparent improvement is not reflected in the growth response of birds fed with the treated-PKE.
30137571	6	29	theme	cecal	1220:1224	arg1	microbiota					1226:1235	the cecal microbiota	1216:1235	the cecal microbiota of broiler chickens at 14 d old (day 14) and 28 d old (day 28)	1216:1298	Hence, this study was designed to assess the effects of feeding different forms of PKE; namely untreated PKE (UPKE), enzyme-treated PKE (EPKE), and oligosaccharides extracted from PKE (OligoPKE), on the cecal microbiota of broiler chickens at 14 d old (day 14) and 28 d old (day 28) using 16S rRNA gene high-throughput next-generation sequencing method.
30137571	0	30	theme	Metagenomics	0:11	arg1	analysis					13:20	Metagenomics analysis	0:20	Metagenomics analysis	0:20	Metagenomics analysis reveals significant modulation of cecal microbiota of broilers fed palm kernel expeller diets.
30137571	8	31	theme	EPKE	1644:1647	arg1	group					1649:1653	EPKE group	1644:1653	EPKE group	1644:1653	The relative abundance of phylum Firmicutes, known to be involved in nutrient uptake and absorption in both age groups was higher in the UPKE as compared to EPKE group.
30137571	6	32	from	effects	1062:1068	arg1	microbiota					1226:1235	the cecal microbiota	1216:1235	the cecal microbiota of broiler chickens at 14 d old (day 14) and 28 d old (day 28)	1216:1298	Hence, this study was designed to assess the effects of feeding different forms of PKE; namely untreated PKE (UPKE), enzyme-treated PKE (EPKE), and oligosaccharides extracted from PKE (OligoPKE), on the cecal microbiota of broiler chickens at 14 d old (day 14) and 28 d old (day 28) using 16S rRNA gene high-throughput next-generation sequencing method.
30137571	3	33	theme	intestinal	608:617	arg1	environment					619:629	the intestinal environment	604:629	the intestinal environment	604:629	As chicken's ceca are the most heavily populated with microflora within their gastrointestinal tract, it was hypothesized that any modulation of the intestinal environment by dietary treatments should be reflected by the composition and activities of the cecal microflora.
30137571	7	34	from	changes	1404:1410	arg1	microbiota					1421:1430	cecal microbiota	1415:1430	cecal microbiota of broiler chickens	1415:1450	The results showed that temporal changes in cecal microbiota of broiler chickens were evident on day 14 and day 28.
30137571	2	35	theme	birds	427:431	arg1	response					415:422	the growth response	404:422	the growth response of birds fed with the treated-PKE	404:456	Although enzymatic treatment could alleviate the fiber component and increase the nutritive value of PKE, this apparent improvement is not reflected in the growth response of birds fed with the treated-PKE.
30137571	9	36	theme	Lactobacillus	1849:1861	arg1	growth					1828:1833	growth	1828:1833	growth of indigenous Lactobacillus	1828:1861	In addition, supplementation of OligoPKE increased (P < 0.05) the relative abundance of Lactobacillus on both D14 and D28, signifying its effect as prebiotics in enhancing growth of indigenous Lactobacillus.
30137571	6	37	theme	old	1265:1267	arg1	day					1270:1272	day 14	1270:1275	day 14	1270:1275	Hence, this study was designed to assess the effects of feeding different forms of PKE; namely untreated PKE (UPKE), enzyme-treated PKE (EPKE), and oligosaccharides extracted from PKE (OligoPKE), on the cecal microbiota of broiler chickens at 14 d old (day 14) and 28 d old (day 28) using 16S rRNA gene high-throughput next-generation sequencing method.
30137571	6	37	theme	old	1265:1267	arg1	d					1263:1263	14 d old	1260:1267	14 d old (day 14)	1260:1276	Hence, this study was designed to assess the effects of feeding different forms of PKE; namely untreated PKE (UPKE), enzyme-treated PKE (EPKE), and oligosaccharides extracted from PKE (OligoPKE), on the cecal microbiota of broiler chickens at 14 d old (day 14) and 28 d old (day 28) using 16S rRNA gene high-throughput next-generation sequencing method.
30137571	6	38	theme	16S	1306:1308	arg1	method					1363:1368	16S rRNA gene high-throughput next-generation sequencing method	1306:1368	16S rRNA gene high-throughput next-generation sequencing method	1306:1368	Hence, this study was designed to assess the effects of feeding different forms of PKE; namely untreated PKE (UPKE), enzyme-treated PKE (EPKE), and oligosaccharides extracted from PKE (OligoPKE), on the cecal microbiota of broiler chickens at 14 d old (day 14) and 28 d old (day 28) using 16S rRNA gene high-throughput next-generation sequencing method.
30137571	1	39	theme	broiler	200:206	arg1	diets					208:212	broiler diets	200:212	broiler diets	200:212	The potential use of palm kernel expeller (PKE) as an alternative energy source in broiler diets is limited by the high fiber content.
30137571	10	40	theme	cecal	1888:1892	arg1	microbiota					1894:1903	cecal microbiota	1888:1903	cecal microbiota	1888:1903	Our results showed that cecal microbiota was significantly modulated by dietary treatments and that the lower relative abundance of phylum Firmicutes in chickens fed with EPKE could be a reason why broiler chickens fed with EPKE of higher metabolizable energy (ME) content did not show improvement in their growth performance.
30137571	4	41	theme	microbiota	769:778	arg1	composition					780:790	cecal microbiota composition	763:790	cecal microbiota composition	763:790	There is a correlation between cecal microbiota composition and the efficiency of the host to extract energy from the diet and to deposit that energy into improved feed conversion ratio.
30137571	2	42	theme	nutritive	334:342	arg1	value					344:348	the nutritive value	330:348	the nutritive value of PKE	330:355	Although enzymatic treatment could alleviate the fiber component and increase the nutritive value of PKE, this apparent improvement is not reflected in the growth response of birds fed with the treated-PKE.
30137571	8	43	theme	relative	1491:1498	arg1	abundance					1500:1508	The relative abundance	1487:1508	The relative abundance of phylum Firmicutes, known to be involved in nutrient uptake and absorption in both age groups	1487:1604	The relative abundance of phylum Firmicutes, known to be involved in nutrient uptake and absorption in both age groups was higher in the UPKE as compared to EPKE group.
30137571	8	43	theme	relative	1491:1498	arg1	higher					1610:1615	higher	1610:1615	higher	1610:1615	The relative abundance of phylum Firmicutes, known to be involved in nutrient uptake and absorption in both age groups was higher in the UPKE as compared to EPKE group.
30137571	4	44	theme	extract	826:832	arg1	energy					834:839	extract energy	826:839	extract energy from the diet	826:853	There is a correlation between cecal microbiota composition and the efficiency of the host to extract energy from the diet and to deposit that energy into improved feed conversion ratio.
30137571	2	45	theme	enzymatic	261:269	arg1	treatment					271:279	enzymatic treatment	261:279	enzymatic treatment	261:279	Although enzymatic treatment could alleviate the fiber component and increase the nutritive value of PKE, this apparent improvement is not reflected in the growth response of birds fed with the treated-PKE.
30137571	6	46	theme	old	1287:1289	arg1	day					1292:1294	day 28	1292:1297	day 28	1292:1297	Hence, this study was designed to assess the effects of feeding different forms of PKE; namely untreated PKE (UPKE), enzyme-treated PKE (EPKE), and oligosaccharides extracted from PKE (OligoPKE), on the cecal microbiota of broiler chickens at 14 d old (day 14) and 28 d old (day 28) using 16S rRNA gene high-throughput next-generation sequencing method.
30137571	6	46	theme	old	1287:1289	arg1	d					1285:1285	28 d old	1282:1289	28 d old (day 28)	1282:1298	Hence, this study was designed to assess the effects of feeding different forms of PKE; namely untreated PKE (UPKE), enzyme-treated PKE (EPKE), and oligosaccharides extracted from PKE (OligoPKE), on the cecal microbiota of broiler chickens at 14 d old (day 14) and 28 d old (day 28) using 16S rRNA gene high-throughput next-generation sequencing method.
30137571	1	47	theme	high	232:235	arg1	content					243:249	the high fiber content	228:249	the high fiber content	228:249	The potential use of palm kernel expeller (PKE) as an alternative energy source in broiler diets is limited by the high fiber content.
30137571	6	48	theme	PKE	1100:1102	arg1	PKE					1122:1124	namely untreated PKE	1105:1124	namely untreated PKE (UPKE)	1105:1131	Hence, this study was designed to assess the effects of feeding different forms of PKE; namely untreated PKE (UPKE), enzyme-treated PKE (EPKE), and oligosaccharides extracted from PKE (OligoPKE), on the cecal microbiota of broiler chickens at 14 d old (day 14) and 28 d old (day 28) using 16S rRNA gene high-throughput next-generation sequencing method.
30137571	6	48	theme	PKE	1100:1102	arg1	PKE					1149:1151	enzyme-treated PKE	1134:1151	enzyme-treated PKE (EPKE)	1134:1158	Hence, this study was designed to assess the effects of feeding different forms of PKE; namely untreated PKE (UPKE), enzyme-treated PKE (EPKE), and oligosaccharides extracted from PKE (OligoPKE), on the cecal microbiota of broiler chickens at 14 d old (day 14) and 28 d old (day 28) using 16S rRNA gene high-throughput next-generation sequencing method.
30137571	6	48	theme	PKE	1100:1102	arg1	forms					1091:1095	different forms	1081:1095	different forms of PKE	1081:1102	Hence, this study was designed to assess the effects of feeding different forms of PKE; namely untreated PKE (UPKE), enzyme-treated PKE (EPKE), and oligosaccharides extracted from PKE (OligoPKE), on the cecal microbiota of broiler chickens at 14 d old (day 14) and 28 d old (day 28) using 16S rRNA gene high-throughput next-generation sequencing method.
30137571	6	48	theme	PKE	1100:1102	arg1	EPKE					1154:1157	EPKE	1154:1157	EPKE	1154:1157	Hence, this study was designed to assess the effects of feeding different forms of PKE; namely untreated PKE (UPKE), enzyme-treated PKE (EPKE), and oligosaccharides extracted from PKE (OligoPKE), on the cecal microbiota of broiler chickens at 14 d old (day 14) and 28 d old (day 28) using 16S rRNA gene high-throughput next-generation sequencing method.
30137571	6	48	theme	PKE	1100:1102	arg1	UPKE					1127:1130	UPKE	1127:1130	UPKE	1127:1130	Hence, this study was designed to assess the effects of feeding different forms of PKE; namely untreated PKE (UPKE), enzyme-treated PKE (EPKE), and oligosaccharides extracted from PKE (OligoPKE), on the cecal microbiota of broiler chickens at 14 d old (day 14) and 28 d old (day 28) using 16S rRNA gene high-throughput next-generation sequencing method.
30137571	6	48	theme	PKE	1100:1102	arg1	oligosaccharides					1165:1180	oligosaccharides	1165:1180	oligosaccharides extracted from PKE (OligoPKE)	1165:1210	Hence, this study was designed to assess the effects of feeding different forms of PKE; namely untreated PKE (UPKE), enzyme-treated PKE (EPKE), and oligosaccharides extracted from PKE (OligoPKE), on the cecal microbiota of broiler chickens at 14 d old (day 14) and 28 d old (day 28) using 16S rRNA gene high-throughput next-generation sequencing method.
30137571	8	49	theme	Firmicutes	1520:1529	arg1	abundance					1500:1508	The relative abundance	1487:1508	The relative abundance of phylum Firmicutes, known to be involved in nutrient uptake and absorption in both age groups	1487:1604	The relative abundance of phylum Firmicutes, known to be involved in nutrient uptake and absorption in both age groups was higher in the UPKE as compared to EPKE group.
30137571	8	49	theme	Firmicutes	1520:1529	arg1	higher					1610:1615	higher	1610:1615	higher	1610:1615	The relative abundance of phylum Firmicutes, known to be involved in nutrient uptake and absorption in both age groups was higher in the UPKE as compared to EPKE group.
30137571	0	50	theme	cecal	56:60	arg1	microbiota					62:71	cecal microbiota	56:71	cecal microbiota of broilers fed palm kernel expeller diets	56:114	Metagenomics analysis reveals significant modulation of cecal microbiota of broilers fed palm kernel expeller diets.
30137571	9	51	theme	P	1708:1708	arg1	<					1710:1710	P < 0.05	1708:1715	P < 0.05	1708:1715	In addition, supplementation of OligoPKE increased (P < 0.05) the relative abundance of Lactobacillus on both D14 and D28, signifying its effect as prebiotics in enhancing growth of indigenous Lactobacillus.
30137571	4	52	theme	host	818:821	arg1	composition					780:790	cecal microbiota composition	763:790	cecal microbiota composition	763:790	There is a correlation between cecal microbiota composition and the efficiency of the host to extract energy from the diet and to deposit that energy into improved feed conversion ratio.
30137571	4	52	theme	host	818:821	arg1	efficiency					800:809	the efficiency	796:809	the efficiency of the host to extract energy from the diet and to deposit that energy into improved feed conversion ratio	796:916	There is a correlation between cecal microbiota composition and the efficiency of the host to extract energy from the diet and to deposit that energy into improved feed conversion ratio.
30137571	10	53	theme	dietary	1936:1942	arg1	treatments					1944:1953	dietary treatments	1936:1953	dietary treatments	1936:1953	Our results showed that cecal microbiota was significantly modulated by dietary treatments and that the lower relative abundance of phylum Firmicutes in chickens fed with EPKE could be a reason why broiler chickens fed with EPKE of higher metabolizable energy (ME) content did not show improvement in their growth performance.
30137571	3	54	theme	microflora	720:729	arg1	composition					680:690	composition	680:690	composition	680:690	As chicken's ceca are the most heavily populated with microflora within their gastrointestinal tract, it was hypothesized that any modulation of the intestinal environment by dietary treatments should be reflected by the composition and activities of the cecal microflora.
30137571	3	54	theme	microflora	720:729	arg1	activities					696:705	activities	696:705	activities	696:705	As chicken's ceca are the most heavily populated with microflora within their gastrointestinal tract, it was hypothesized that any modulation of the intestinal environment by dietary treatments should be reflected by the composition and activities of the cecal microflora.
30137571	6	55	theme	next-generation	1336:1350	arg1	method					1363:1368	16S rRNA gene high-throughput next-generation sequencing method	1306:1368	16S rRNA gene high-throughput next-generation sequencing method	1306:1368	Hence, this study was designed to assess the effects of feeding different forms of PKE; namely untreated PKE (UPKE), enzyme-treated PKE (EPKE), and oligosaccharides extracted from PKE (OligoPKE), on the cecal microbiota of broiler chickens at 14 d old (day 14) and 28 d old (day 28) using 16S rRNA gene high-throughput next-generation sequencing method.
30137571	1	56	theme	alternative	171:181	arg1	source					190:195	an alternative energy source	168:195	an alternative energy source in broiler diets	168:212	The potential use of palm kernel expeller (PKE) as an alternative energy source in broiler diets is limited by the high fiber content.
30137571	10	57	theme	higher	2096:2101	arg1	content					2129:2135	higher metabolizable energy (ME) content	2096:2135	higher metabolizable energy (ME) content	2096:2135	Our results showed that cecal microbiota was significantly modulated by dietary treatments and that the lower relative abundance of phylum Firmicutes in chickens fed with EPKE could be a reason why broiler chickens fed with EPKE of higher metabolizable energy (ME) content did not show improvement in their growth performance.
30137571	6	58	theme	gene	1315:1318	arg1	method					1363:1368	16S rRNA gene high-throughput next-generation sequencing method	1306:1368	16S rRNA gene high-throughput next-generation sequencing method	1306:1368	Hence, this study was designed to assess the effects of feeding different forms of PKE; namely untreated PKE (UPKE), enzyme-treated PKE (EPKE), and oligosaccharides extracted from PKE (OligoPKE), on the cecal microbiota of broiler chickens at 14 d old (day 14) and 28 d old (day 28) using 16S rRNA gene high-throughput next-generation sequencing method.
30137571	10	59	from	abundance	1983:1991	arg1	chickens					2017:2024	chickens	2017:2024	chickens fed with EPKE	2017:2038	Our results showed that cecal microbiota was significantly modulated by dietary treatments and that the lower relative abundance of phylum Firmicutes in chickens fed with EPKE could be a reason why broiler chickens fed with EPKE of higher metabolizable energy (ME) content did not show improvement in their growth performance.
30137571	10	60	theme	growth	2171:2176	arg1	performance					2178:2188	their growth performance	2165:2188	their growth performance	2165:2188	Our results showed that cecal microbiota was significantly modulated by dietary treatments and that the lower relative abundance of phylum Firmicutes in chickens fed with EPKE could be a reason why broiler chickens fed with EPKE of higher metabolizable energy (ME) content did not show improvement in their growth performance.
30137571	6	61	theme	broiler	1240:1246	arg1	chickens					1248:1255	broiler chickens	1240:1255	broiler chickens	1240:1255	Hence, this study was designed to assess the effects of feeding different forms of PKE; namely untreated PKE (UPKE), enzyme-treated PKE (EPKE), and oligosaccharides extracted from PKE (OligoPKE), on the cecal microbiota of broiler chickens at 14 d old (day 14) and 28 d old (day 28) using 16S rRNA gene high-throughput next-generation sequencing method.
30137571	3	62	theme	gastrointestinal	537:552	arg1	tract					554:558	their gastrointestinal tract	531:558	their gastrointestinal tract	531:558	As chicken's ceca are the most heavily populated with microflora within their gastrointestinal tract, it was hypothesized that any modulation of the intestinal environment by dietary treatments should be reflected by the composition and activities of the cecal microflora.
30137571	7	63	theme	broiler	1435:1441	arg1	chickens					1443:1450	broiler chickens	1435:1450	broiler chickens	1435:1450	The results showed that temporal changes in cecal microbiota of broiler chickens were evident on day 14 and day 28.
30137571	10	64	theme	ME	2125:2126	arg1	content					2129:2135	higher metabolizable energy (ME) content	2096:2135	higher metabolizable energy (ME) content	2096:2135	Our results showed that cecal microbiota was significantly modulated by dietary treatments and that the lower relative abundance of phylum Firmicutes in chickens fed with EPKE could be a reason why broiler chickens fed with EPKE of higher metabolizable energy (ME) content did not show improvement in their growth performance.
30137571	9	65	theme	Lactobacillus	1744:1756	arg1	abundance					1731:1739	the relative abundance	1718:1739	the relative abundance of Lactobacillus	1718:1756	In addition, supplementation of OligoPKE increased (P < 0.05) the relative abundance of Lactobacillus on both D14 and D28, signifying its effect as prebiotics in enhancing growth of indigenous Lactobacillus.
30137571	5	66	theme	broilers	988:995	arg1	microflora					974:983	cecal microflora	968:983	cecal microflora of broilers fed with PKE diets	968:1014	At present, little is known about the changes on cecal microflora of broilers fed with PKE diets.
30137571	10	67	theme	energy	2117:2122	arg1	content					2129:2135	higher metabolizable energy (ME) content	2096:2135	higher metabolizable energy (ME) content	2096:2135	Our results showed that cecal microbiota was significantly modulated by dietary treatments and that the lower relative abundance of phylum Firmicutes in chickens fed with EPKE could be a reason why broiler chickens fed with EPKE of higher metabolizable energy (ME) content did not show improvement in their growth performance.
30137571	0	68	theme	broilers	76:83	arg1	microbiota					62:71	cecal microbiota	56:71	cecal microbiota of broilers fed palm kernel expeller diets	56:114	Metagenomics analysis reveals significant modulation of cecal microbiota of broilers fed palm kernel expeller diets.
30137571	4	69	from	diet	850:853	arg1	energy					834:839	extract energy	826:839	extract energy from the diet	826:853	There is a correlation between cecal microbiota composition and the efficiency of the host to extract energy from the diet and to deposit that energy into improved feed conversion ratio.
30137571	1	70	theme	palm	138:141	arg1	PKE					160:162	PKE	160:162	PKE	160:162	The potential use of palm kernel expeller (PKE) as an alternative energy source in broiler diets is limited by the high fiber content.
30137571	1	70	theme	palm	138:141	arg1	expeller					150:157	palm kernel expeller	138:157	palm kernel expeller (PKE)	138:163	The potential use of palm kernel expeller (PKE) as an alternative energy source in broiler diets is limited by the high fiber content.
30137571	10	71	theme	relative	1974:1981	arg1	reason					2051:2056	a reason why broiler chickens fed with EPKE of higher metabolizable energy (ME) content did not show improvement in their growth performance	2049:2188	a reason why broiler chickens fed with EPKE of higher metabolizable energy (ME) content did not show improvement in their growth performance	2049:2188	Our results showed that cecal microbiota was significantly modulated by dietary treatments and that the lower relative abundance of phylum Firmicutes in chickens fed with EPKE could be a reason why broiler chickens fed with EPKE of higher metabolizable energy (ME) content did not show improvement in their growth performance.
30137571	10	71	theme	relative	1974:1981	arg1	abundance					1983:1991	the lower relative abundance	1964:1991	the lower relative abundance of phylum Firmicutes in chickens fed with EPKE	1964:2038	Our results showed that cecal microbiota was significantly modulated by dietary treatments and that the lower relative abundance of phylum Firmicutes in chickens fed with EPKE could be a reason why broiler chickens fed with EPKE of higher metabolizable energy (ME) content did not show improvement in their growth performance.
30137571	0	72	theme	palm	89:92	arg1	diets					110:114	palm kernel expeller diets	89:114	palm kernel expeller diets	89:114	Metagenomics analysis reveals significant modulation of cecal microbiota of broilers fed palm kernel expeller diets.
30137571	1	73	theme	expeller	150:157	arg1	use					131:133	The potential use	117:133	The potential use of palm kernel expeller (PKE) as an alternative energy source in broiler diets	117:212	The potential use of palm kernel expeller (PKE) as an alternative energy source in broiler diets is limited by the high fiber content.
30137571	7	74	theme	temporal	1395:1402	arg1	changes					1404:1410	temporal changes	1395:1410	temporal changes in cecal microbiota of broiler chickens	1395:1450	The results showed that temporal changes in cecal microbiota of broiler chickens were evident on day 14 and day 28.
30137571	10	75	theme	content	2129:2135	arg1	EPKE					2088:2091	EPKE	2088:2091	EPKE of higher metabolizable energy (ME) content	2088:2135	Our results showed that cecal microbiota was significantly modulated by dietary treatments and that the lower relative abundance of phylum Firmicutes in chickens fed with EPKE could be a reason why broiler chickens fed with EPKE of higher metabolizable energy (ME) content did not show improvement in their growth performance.
30137571	0	76	theme	expeller	101:108	arg1	diets					110:114	palm kernel expeller diets	89:114	palm kernel expeller diets	89:114	Metagenomics analysis reveals significant modulation of cecal microbiota of broilers fed palm kernel expeller diets.
30137571	4	77	theme	feed	896:899	arg1	ratio					912:916	improved feed conversion ratio	887:916	improved feed conversion ratio	887:916	There is a correlation between cecal microbiota composition and the efficiency of the host to extract energy from the diet and to deposit that energy into improved feed conversion ratio.
30137571	10	78	theme	Firmicutes	2003:2012	arg1	reason					2051:2056	a reason why broiler chickens fed with EPKE of higher metabolizable energy (ME) content did not show improvement in their growth performance	2049:2188	a reason why broiler chickens fed with EPKE of higher metabolizable energy (ME) content did not show improvement in their growth performance	2049:2188	Our results showed that cecal microbiota was significantly modulated by dietary treatments and that the lower relative abundance of phylum Firmicutes in chickens fed with EPKE could be a reason why broiler chickens fed with EPKE of higher metabolizable energy (ME) content did not show improvement in their growth performance.
30137571	10	78	theme	Firmicutes	2003:2012	arg1	abundance					1983:1991	the lower relative abundance	1964:1991	the lower relative abundance of phylum Firmicutes in chickens fed with EPKE	1964:2038	Our results showed that cecal microbiota was significantly modulated by dietary treatments and that the lower relative abundance of phylum Firmicutes in chickens fed with EPKE could be a reason why broiler chickens fed with EPKE of higher metabolizable energy (ME) content did not show improvement in their growth performance.
30137571	8	79	theme	nutrient	1556:1563	arg1	uptake					1565:1570	nutrient uptake	1556:1570	nutrient uptake	1556:1570	The relative abundance of phylum Firmicutes, known to be involved in nutrient uptake and absorption in both age groups was higher in the UPKE as compared to EPKE group.
30137571	2	80	theme	growth	408:413	arg1	response					415:422	the growth response	404:422	the growth response of birds fed with the treated-PKE	404:456	Although enzymatic treatment could alleviate the fiber component and increase the nutritive value of PKE, this apparent improvement is not reflected in the growth response of birds fed with the treated-PKE.
30137571	2	81	theme	PKE	353:355	arg1	value					344:348	the nutritive value	330:348	the nutritive value of PKE	330:355	Although enzymatic treatment could alleviate the fiber component and increase the nutritive value of PKE, this apparent improvement is not reflected in the growth response of birds fed with the treated-PKE.
30137571	0	82	theme	significant	30:40	arg1	modulation					42:51	significant modulation	30:51	significant modulation of cecal microbiota of broilers fed palm kernel expeller diets	30:114	Metagenomics analysis reveals significant modulation of cecal microbiota of broilers fed palm kernel expeller diets.
30137571	9	83	theme	indigenous	1838:1847	arg1	Lactobacillus					1849:1861	indigenous Lactobacillus	1838:1861	indigenous Lactobacillus	1838:1861	In addition, supplementation of OligoPKE increased (P < 0.05) the relative abundance of Lactobacillus on both D14 and D28, signifying its effect as prebiotics in enhancing growth of indigenous Lactobacillus.
30137571	0	84	theme	microbiota	62:71	arg1	modulation					42:51	significant modulation	30:51	significant modulation of cecal microbiota of broilers fed palm kernel expeller diets	30:114	Metagenomics analysis reveals significant modulation of cecal microbiota of broilers fed palm kernel expeller diets.
30137571	3	85	dep	composition	680:690	arg1	the					676:678	the	676:678	the	676:678	As chicken's ceca are the most heavily populated with microflora within their gastrointestinal tract, it was hypothesized that any modulation of the intestinal environment by dietary treatments should be reflected by the composition and activities of the cecal microflora.
30137571	5	86	theme	cecal	968:972	arg1	microflora					974:983	cecal microflora	968:983	cecal microflora of broilers fed with PKE diets	968:1014	At present, little is known about the changes on cecal microflora of broilers fed with PKE diets.
30137571	3	87	theme	dietary	634:640	arg1	treatments					642:651	dietary treatments	634:651	dietary treatments	634:651	As chicken's ceca are the most heavily populated with microflora within their gastrointestinal tract, it was hypothesized that any modulation of the intestinal environment by dietary treatments should be reflected by the composition and activities of the cecal microflora.
30137571	6	88	theme	rRNA	1310:1313	arg1	method					1363:1368	16S rRNA gene high-throughput next-generation sequencing method	1306:1368	16S rRNA gene high-throughput next-generation sequencing method	1306:1368	Hence, this study was designed to assess the effects of feeding different forms of PKE; namely untreated PKE (UPKE), enzyme-treated PKE (EPKE), and oligosaccharides extracted from PKE (OligoPKE), on the cecal microbiota of broiler chickens at 14 d old (day 14) and 28 d old (day 28) using 16S rRNA gene high-throughput next-generation sequencing method.
30137571	10	89	theme	broiler	2062:2068	arg1	chickens					2070:2077	broiler chickens	2062:2077	broiler chickens fed with EPKE of higher metabolizable energy (ME) content	2062:2135	Our results showed that cecal microbiota was significantly modulated by dietary treatments and that the lower relative abundance of phylum Firmicutes in chickens fed with EPKE could be a reason why broiler chickens fed with EPKE of higher metabolizable energy (ME) content did not show improvement in their growth performance.
30137571	3	90	theme	environment	619:629	arg1	modulation					590:599	any modulation	586:599	any modulation of the intestinal environment by dietary treatments	586:651	As chicken's ceca are the most heavily populated with microflora within their gastrointestinal tract, it was hypothesized that any modulation of the intestinal environment by dietary treatments should be reflected by the composition and activities of the cecal microflora.
30137571	2	91	theme	fiber	301:305	arg1	component					307:315	the fiber component	297:315	the fiber component	297:315	Although enzymatic treatment could alleviate the fiber component and increase the nutritive value of PKE, this apparent improvement is not reflected in the growth response of birds fed with the treated-PKE.
30137571	4	92	theme	cecal	763:767	arg1	composition					780:790	cecal microbiota composition	763:790	cecal microbiota composition	763:790	There is a correlation between cecal microbiota composition and the efficiency of the host to extract energy from the diet and to deposit that energy into improved feed conversion ratio.
30309566	1	0	theme	methods	289:295	arg1	potential					190:198	the potential	186:198	the potential of three cultivars ('Amadeus', 'Colossal Meidiland' and 'Rosanna') and three traditional methods of preparation (fresh/air-dried petals extracted in 50% ethanol or aqueous sucrose syrup)	186:385	Chemical composition and colorimetric parameters of alcoholic liqueurs prepared from rose petals were evaluated by comparing the potential of three cultivars ('Amadeus', 'Colossal Meidiland' and 'Rosanna') and three traditional methods of preparation (fresh/air-dried petals extracted in 50% ethanol or aqueous sucrose syrup).
30309566	4	1	from	petals	731:736	arg1	extracts					707:714	extracts	707:714	extracts from 'Amadeus' petals	707:736	The highest concentrations of anthocyanins were determined in extracts from 'Amadeus' petals, followed by 'Colossal Meidiland' and finally, 'Rosanna'.
30309566	3	2	theme	performance	451:461	arg1	spectrometry					490:501	High performance liquid chromatography/mass spectrometry	446:501	High performance liquid chromatography/mass spectrometry	446:501	High performance liquid chromatography/mass spectrometry was used to confirm the presence of 6 anthocyanins, 4 flavanols, 4 phenolic acids, 2 hydrolysable tannins and 31 flavonols in petal liqueurs.
30309566	5	3	theme	colour	835:840	arg1	characteristics					842:856	optimal colour characteristics	827:856	optimal colour characteristics	827:856	The best extraction yields and optimal colour characteristics were achieved by ethanolic extraction of dry petals followed by fresh petal extraction in ethanol and, finally, extraction in sucrose syrup.
30309566	3	4	theme	petal	629:633	arg1	liqueurs					635:642	petal liqueurs	629:642	petal liqueurs	629:642	High performance liquid chromatography/mass spectrometry was used to confirm the presence of 6 anthocyanins, 4 flavanols, 4 phenolic acids, 2 hydrolysable tannins and 31 flavonols in petal liqueurs.
30309566	3	5	theme	liquid	463:468	arg1	spectrometry					490:501	High performance liquid chromatography/mass spectrometry	446:501	High performance liquid chromatography/mass spectrometry	446:501	High performance liquid chromatography/mass spectrometry was used to confirm the presence of 6 anthocyanins, 4 flavanols, 4 phenolic acids, 2 hydrolysable tannins and 31 flavonols in petal liqueurs.
30309566	5	6	theme	sucrose	984:990	arg1	syrup					992:996	sucrose syrup	984:996	sucrose syrup	984:996	The best extraction yields and optimal colour characteristics were achieved by ethanolic extraction of dry petals followed by fresh petal extraction in ethanol and, finally, extraction in sucrose syrup.
30309566	1	7	theme	preparation	300:310	arg1	Rosanna					257:263	Rosanna	257:263	Rosanna	257:263	Chemical composition and colorimetric parameters of alcoholic liqueurs prepared from rose petals were evaluated by comparing the potential of three cultivars ('Amadeus', 'Colossal Meidiland' and 'Rosanna') and three traditional methods of preparation (fresh/air-dried petals extracted in 50% ethanol or aqueous sucrose syrup).
30309566	1	7	theme	preparation	300:310	arg1	methods					289:295	three traditional methods	271:295	three traditional methods of preparation	271:310	Chemical composition and colorimetric parameters of alcoholic liqueurs prepared from rose petals were evaluated by comparing the potential of three cultivars ('Amadeus', 'Colossal Meidiland' and 'Rosanna') and three traditional methods of preparation (fresh/air-dried petals extracted in 50% ethanol or aqueous sucrose syrup).
30309566	1	7	theme	preparation	300:310	arg1	Meidiland					241:249	Meidiland	241:249	Meidiland	241:249	Chemical composition and colorimetric parameters of alcoholic liqueurs prepared from rose petals were evaluated by comparing the potential of three cultivars ('Amadeus', 'Colossal Meidiland' and 'Rosanna') and three traditional methods of preparation (fresh/air-dried petals extracted in 50% ethanol or aqueous sucrose syrup).
30309566	1	7	theme	preparation	300:310	arg1	cultivars					209:217	three cultivars	203:217	three cultivars ('Amadeus', 'Colossal Meidiland' and 'Rosanna')	203:265	Chemical composition and colorimetric parameters of alcoholic liqueurs prepared from rose petals were evaluated by comparing the potential of three cultivars ('Amadeus', 'Colossal Meidiland' and 'Rosanna') and three traditional methods of preparation (fresh/air-dried petals extracted in 50% ethanol or aqueous sucrose syrup).
30309566	6	8	theme	round	1109:1113	arg1	characteristics					1115:1129	the best all round characteristics	1096:1129	the best all round characteristics	1096:1129	Air-dehydration of 'Amadeus' petals prior to extraction in 50% ethanol yielded rose liqueur with the best all round characteristics.
30309566	5	9	theme	fresh	922:926	arg1	extraction					934:943	fresh petal extraction	922:943	fresh petal extraction in ethanol	922:954	The best extraction yields and optimal colour characteristics were achieved by ethanolic extraction of dry petals followed by fresh petal extraction in ethanol and, finally, extraction in sucrose syrup.
30309566	0	10	from	rich	42:45	arg1	phenolics					50:58	phenolics	50:58	phenolics	50:58	Traditional rose liqueur - A pink delight rich in phenolics.
30309566	6	11	theme	all	1105:1107	arg1	characteristics					1115:1129	the best all round characteristics	1096:1129	the best all round characteristics	1096:1129	Air-dehydration of 'Amadeus' petals prior to extraction in 50% ethanol yielded rose liqueur with the best all round characteristics.
30309566	6	12	from	Air-dehydration	999:1013	arg1	ethanol					1062:1068	50% ethanol	1058:1068	50% ethanol	1058:1068	Air-dehydration of 'Amadeus' petals prior to extraction in 50% ethanol yielded rose liqueur with the best all round characteristics.
30309566	5	13	theme	dry	899:901	arg1	petals					903:908	dry petals	899:908	dry petals followed by fresh petal extraction in ethanol and, finally, extraction in sucrose syrup	899:996	The best extraction yields and optimal colour characteristics were achieved by ethanolic extraction of dry petals followed by fresh petal extraction in ethanol and, finally, extraction in sucrose syrup.
30309566	6	14	theme	petals	1028:1033	arg1	Air-dehydration					999:1013	Air-dehydration	999:1013	Air-dehydration of 'Amadeus' petals prior to extraction in 50% ethanol	999:1068	Air-dehydration of 'Amadeus' petals prior to extraction in 50% ethanol yielded rose liqueur with the best all round characteristics.
30309566	1	15	theme	fresh/air-dried	313:327	arg1	petals					329:334	fresh/air-dried petals	313:334	fresh/air-dried petals extracted in 50% ethanol or aqueous sucrose syrup	313:384	Chemical composition and colorimetric parameters of alcoholic liqueurs prepared from rose petals were evaluated by comparing the potential of three cultivars ('Amadeus', 'Colossal Meidiland' and 'Rosanna') and three traditional methods of preparation (fresh/air-dried petals extracted in 50% ethanol or aqueous sucrose syrup).
30309566	5	16	theme	petals	903:908	arg1	extraction					885:894	ethanolic extraction	875:894	ethanolic extraction of dry petals followed by fresh petal extraction in ethanol and, finally, extraction in sucrose syrup	875:996	The best extraction yields and optimal colour characteristics were achieved by ethanolic extraction of dry petals followed by fresh petal extraction in ethanol and, finally, extraction in sucrose syrup.
30309566	5	17	from	extraction	934:943	arg1	ethanol					948:954	ethanol	948:954	ethanol	948:954	The best extraction yields and optimal colour characteristics were achieved by ethanolic extraction of dry petals followed by fresh petal extraction in ethanol and, finally, extraction in sucrose syrup.
30309566	5	17	from	extraction	934:943	arg1	syrup					992:996	sucrose syrup	984:996	sucrose syrup	984:996	The best extraction yields and optimal colour characteristics were achieved by ethanolic extraction of dry petals followed by fresh petal extraction in ethanol and, finally, extraction in sucrose syrup.
30309566	3	18	used	used	507:510	arg2	spectrometry					490:501	High performance liquid chromatography/mass spectrometry	446:501	High performance liquid chromatography/mass spectrometry	446:501	High performance liquid chromatography/mass spectrometry was used to confirm the presence of 6 anthocyanins, 4 flavanols, 4 phenolic acids, 2 hydrolysable tannins and 31 flavonols in petal liqueurs.
30309566	1	19	theme	Chemical	61:68	arg1	composition					70:80	Chemical composition	61:80	Chemical composition	61:80	Chemical composition and colorimetric parameters of alcoholic liqueurs prepared from rose petals were evaluated by comparing the potential of three cultivars ('Amadeus', 'Colossal Meidiland' and 'Rosanna') and three traditional methods of preparation (fresh/air-dried petals extracted in 50% ethanol or aqueous sucrose syrup).
30309566	3	20	attach	presence	527:534	arg2	acids					579:583	4 phenolic acids	568:583	4 phenolic acids	568:583	High performance liquid chromatography/mass spectrometry was used to confirm the presence of 6 anthocyanins, 4 flavanols, 4 phenolic acids, 2 hydrolysable tannins and 31 flavonols in petal liqueurs.
30309566	3	20	attach	presence	527:534	arg2	flavonols					616:624	31 flavonols	613:624	31 flavonols	613:624	High performance liquid chromatography/mass spectrometry was used to confirm the presence of 6 anthocyanins, 4 flavanols, 4 phenolic acids, 2 hydrolysable tannins and 31 flavonols in petal liqueurs.
30309566	3	20	attach	presence	527:534	arg1	liqueurs					635:642	petal liqueurs	629:642	petal liqueurs	629:642	High performance liquid chromatography/mass spectrometry was used to confirm the presence of 6 anthocyanins, 4 flavanols, 4 phenolic acids, 2 hydrolysable tannins and 31 flavonols in petal liqueurs.
30309566	3	20	attach	presence	527:534	arg2	anthocyanins					541:552	6 anthocyanins	539:552	6 anthocyanins	539:552	High performance liquid chromatography/mass spectrometry was used to confirm the presence of 6 anthocyanins, 4 flavanols, 4 phenolic acids, 2 hydrolysable tannins and 31 flavonols in petal liqueurs.
30309566	3	20	attach	presence	527:534	arg2	flavanols					557:565	4 flavanols	555:565	4 flavanols	555:565	High performance liquid chromatography/mass spectrometry was used to confirm the presence of 6 anthocyanins, 4 flavanols, 4 phenolic acids, 2 hydrolysable tannins and 31 flavonols in petal liqueurs.
30309566	3	20	attach	presence	527:534	arg2	tannins					601:607	2 hydrolysable tannins	586:607	2 hydrolysable tannins	586:607	High performance liquid chromatography/mass spectrometry was used to confirm the presence of 6 anthocyanins, 4 flavanols, 4 phenolic acids, 2 hydrolysable tannins and 31 flavonols in petal liqueurs.
30309566	0	21	theme	rose	12:15	arg1	liqueur					17:23	Traditional rose liqueur	0:23	Traditional rose liqueur	0:23	Traditional rose liqueur - A pink delight rich in phenolics.
30309566	6	22	with	liqueur	1083:1089	arg1	characteristics					1115:1129	the best all round characteristics	1096:1129	the best all round characteristics	1096:1129	Air-dehydration of 'Amadeus' petals prior to extraction in 50% ethanol yielded rose liqueur with the best all round characteristics.
30309566	0	23	theme	Traditional	0:10	arg1	liqueur					17:23	Traditional rose liqueur	0:23	Traditional rose liqueur	0:23	Traditional rose liqueur - A pink delight rich in phenolics.
30309566	4	24	dep	determined	693:702	arg1	followed					739:746	followed	739:746	followed by 'Colossal Meidiland'	739:770	The highest concentrations of anthocyanins were determined in extracts from 'Amadeus' petals, followed by 'Colossal Meidiland' and finally, 'Rosanna'.
30309566	6	25	theme	%	1060:1060	arg1	ethanol					1062:1068	50% ethanol	1058:1068	50% ethanol	1058:1068	Air-dehydration of 'Amadeus' petals prior to extraction in 50% ethanol yielded rose liqueur with the best all round characteristics.
30309566	3	26	theme	tannins	601:607	arg1	presence					527:534	the presence	523:534	the presence of 6 anthocyanins, 4 flavanols, 4 phenolic acids, 2 hydrolysable tannins and 31 flavonols in petal liqueurs	523:642	High performance liquid chromatography/mass spectrometry was used to confirm the presence of 6 anthocyanins, 4 flavanols, 4 phenolic acids, 2 hydrolysable tannins and 31 flavonols in petal liqueurs.
30309566	1	27	theme	colorimetric	86:97	arg1	parameters					99:108	colorimetric parameters	86:108	colorimetric parameters	86:108	Chemical composition and colorimetric parameters of alcoholic liqueurs prepared from rose petals were evaluated by comparing the potential of three cultivars ('Amadeus', 'Colossal Meidiland' and 'Rosanna') and three traditional methods of preparation (fresh/air-dried petals extracted in 50% ethanol or aqueous sucrose syrup).
30309566	6	28	theme	rose	1078:1081	arg1	liqueur					1083:1089	rose liqueur	1078:1089	rose liqueur with the best all round characteristics	1078:1129	Air-dehydration of 'Amadeus' petals prior to extraction in 50% ethanol yielded rose liqueur with the best all round characteristics.
30309566	3	29	theme	hydrolysable	588:599	arg1	tannins					601:607	2 hydrolysable tannins	586:607	2 hydrolysable tannins	586:607	High performance liquid chromatography/mass spectrometry was used to confirm the presence of 6 anthocyanins, 4 flavanols, 4 phenolic acids, 2 hydrolysable tannins and 31 flavonols in petal liqueurs.
30309566	3	30	theme	High	446:449	arg1	spectrometry					490:501	High performance liquid chromatography/mass spectrometry	446:501	High performance liquid chromatography/mass spectrometry	446:501	High performance liquid chromatography/mass spectrometry was used to confirm the presence of 6 anthocyanins, 4 flavanols, 4 phenolic acids, 2 hydrolysable tannins and 31 flavonols in petal liqueurs.
30309566	5	31	theme	best	800:803	arg1	yields					816:821	The best extraction yields	796:821	The best extraction yields	796:821	The best extraction yields and optimal colour characteristics were achieved by ethanolic extraction of dry petals followed by fresh petal extraction in ethanol and, finally, extraction in sucrose syrup.
30309566	1	32	theme	alcoholic	113:121	arg1	liqueurs					123:130	alcoholic liqueurs	113:130	alcoholic liqueurs prepared from rose petals	113:156	Chemical composition and colorimetric parameters of alcoholic liqueurs prepared from rose petals were evaluated by comparing the potential of three cultivars ('Amadeus', 'Colossal Meidiland' and 'Rosanna') and three traditional methods of preparation (fresh/air-dried petals extracted in 50% ethanol or aqueous sucrose syrup).
30309566	1	33	theme	50	349:350	arg1	%					351:351	%	351:351	%	351:351	Chemical composition and colorimetric parameters of alcoholic liqueurs prepared from rose petals were evaluated by comparing the potential of three cultivars ('Amadeus', 'Colossal Meidiland' and 'Rosanna') and three traditional methods of preparation (fresh/air-dried petals extracted in 50% ethanol or aqueous sucrose syrup).
30309566	2	34	theme	room	416:419	arg1	temperature					421:431	room temperature	416:431	room temperature for 2 weeks	416:443	Extraction was performed at room temperature for 2 weeks.
30309566	3	35	theme	acids	579:583	arg1	presence					527:534	the presence	523:534	the presence of 6 anthocyanins, 4 flavanols, 4 phenolic acids, 2 hydrolysable tannins and 31 flavonols in petal liqueurs	523:642	High performance liquid chromatography/mass spectrometry was used to confirm the presence of 6 anthocyanins, 4 flavanols, 4 phenolic acids, 2 hydrolysable tannins and 31 flavonols in petal liqueurs.
30309566	1	36	theme	%	351:351	arg1	ethanol					353:359	50% ethanol	349:359	50% ethanol	349:359	Chemical composition and colorimetric parameters of alcoholic liqueurs prepared from rose petals were evaluated by comparing the potential of three cultivars ('Amadeus', 'Colossal Meidiland' and 'Rosanna') and three traditional methods of preparation (fresh/air-dried petals extracted in 50% ethanol or aqueous sucrose syrup).
30309566	3	37	theme	flavanols	557:565	arg1	presence					527:534	the presence	523:534	the presence of 6 anthocyanins, 4 flavanols, 4 phenolic acids, 2 hydrolysable tannins and 31 flavonols in petal liqueurs	523:642	High performance liquid chromatography/mass spectrometry was used to confirm the presence of 6 anthocyanins, 4 flavanols, 4 phenolic acids, 2 hydrolysable tannins and 31 flavonols in petal liqueurs.
30309566	1	38	theme	cultivars	209:217	arg1	potential					190:198	the potential	186:198	the potential of three cultivars ('Amadeus', 'Colossal Meidiland' and 'Rosanna') and three traditional methods of preparation (fresh/air-dried petals extracted in 50% ethanol or aqueous sucrose syrup)	186:385	Chemical composition and colorimetric parameters of alcoholic liqueurs prepared from rose petals were evaluated by comparing the potential of three cultivars ('Amadeus', 'Colossal Meidiland' and 'Rosanna') and three traditional methods of preparation (fresh/air-dried petals extracted in 50% ethanol or aqueous sucrose syrup).
30309566	3	39	theme	anthocyanins	541:552	arg1	presence					527:534	the presence	523:534	the presence of 6 anthocyanins, 4 flavanols, 4 phenolic acids, 2 hydrolysable tannins and 31 flavonols in petal liqueurs	523:642	High performance liquid chromatography/mass spectrometry was used to confirm the presence of 6 anthocyanins, 4 flavanols, 4 phenolic acids, 2 hydrolysable tannins and 31 flavonols in petal liqueurs.
30309566	5	40	theme	optimal	827:833	arg1	characteristics					842:856	optimal colour characteristics	827:856	optimal colour characteristics	827:856	The best extraction yields and optimal colour characteristics were achieved by ethanolic extraction of dry petals followed by fresh petal extraction in ethanol and, finally, extraction in sucrose syrup.
30309566	5	41	theme	ethanolic	875:883	arg1	extraction					885:894	ethanolic extraction	875:894	ethanolic extraction of dry petals followed by fresh petal extraction in ethanol and, finally, extraction in sucrose syrup	875:996	The best extraction yields and optimal colour characteristics were achieved by ethanolic extraction of dry petals followed by fresh petal extraction in ethanol and, finally, extraction in sucrose syrup.
30309566	3	42	theme	flavonols	616:624	arg1	presence					527:534	the presence	523:534	the presence of 6 anthocyanins, 4 flavanols, 4 phenolic acids, 2 hydrolysable tannins and 31 flavonols in petal liqueurs	523:642	High performance liquid chromatography/mass spectrometry was used to confirm the presence of 6 anthocyanins, 4 flavanols, 4 phenolic acids, 2 hydrolysable tannins and 31 flavonols in petal liqueurs.
30309566	6	43	theme	50	1058:1059	arg1	%					1060:1060	%	1060:1060	%	1060:1060	Air-dehydration of 'Amadeus' petals prior to extraction in 50% ethanol yielded rose liqueur with the best all round characteristics.
30309566	0	44	from	phenolics	50:58	arg1	rich					42:45	rich	42:45	rich	42:45	Traditional rose liqueur - A pink delight rich in phenolics.
30309566	1	45	theme	aqueous	364:370	arg1	syrup					380:384	aqueous sucrose syrup	364:384	aqueous sucrose syrup	364:384	Chemical composition and colorimetric parameters of alcoholic liqueurs prepared from rose petals were evaluated by comparing the potential of three cultivars ('Amadeus', 'Colossal Meidiland' and 'Rosanna') and three traditional methods of preparation (fresh/air-dried petals extracted in 50% ethanol or aqueous sucrose syrup).
30309566	3	46	theme	phenolic	570:577	arg1	acids					579:583	4 phenolic acids	568:583	4 phenolic acids	568:583	High performance liquid chromatography/mass spectrometry was used to confirm the presence of 6 anthocyanins, 4 flavanols, 4 phenolic acids, 2 hydrolysable tannins and 31 flavonols in petal liqueurs.
30309566	1	47	theme	liqueurs	123:130	arg1	composition					70:80	Chemical composition	61:80	Chemical composition	61:80	Chemical composition and colorimetric parameters of alcoholic liqueurs prepared from rose petals were evaluated by comparing the potential of three cultivars ('Amadeus', 'Colossal Meidiland' and 'Rosanna') and three traditional methods of preparation (fresh/air-dried petals extracted in 50% ethanol or aqueous sucrose syrup).
30309566	1	47	theme	liqueurs	123:130	arg1	parameters					99:108	colorimetric parameters	86:108	colorimetric parameters	86:108	Chemical composition and colorimetric parameters of alcoholic liqueurs prepared from rose petals were evaluated by comparing the potential of three cultivars ('Amadeus', 'Colossal Meidiland' and 'Rosanna') and three traditional methods of preparation (fresh/air-dried petals extracted in 50% ethanol or aqueous sucrose syrup).
30309566	3	48	from	presence	527:534	arg1	liqueurs					635:642	petal liqueurs	629:642	petal liqueurs	629:642	High performance liquid chromatography/mass spectrometry was used to confirm the presence of 6 anthocyanins, 4 flavanols, 4 phenolic acids, 2 hydrolysable tannins and 31 flavonols in petal liqueurs.
30309566	4	49	theme	Amadeus	722:728	arg1	petals					731:736	'Amadeus' petals	721:736	'Amadeus' petals	721:736	The highest concentrations of anthocyanins were determined in extracts from 'Amadeus' petals, followed by 'Colossal Meidiland' and finally, 'Rosanna'.
30309566	1	50	theme	sucrose	372:378	arg1	syrup					380:384	aqueous sucrose syrup	364:384	aqueous sucrose syrup	364:384	Chemical composition and colorimetric parameters of alcoholic liqueurs prepared from rose petals were evaluated by comparing the potential of three cultivars ('Amadeus', 'Colossal Meidiland' and 'Rosanna') and three traditional methods of preparation (fresh/air-dried petals extracted in 50% ethanol or aqueous sucrose syrup).
30309566	1	51	dep	cultivars	209:217	arg1	cultivars					209:217	three cultivars	203:217	three cultivars ('Amadeus', 'Colossal Meidiland' and 'Rosanna')	203:265	Chemical composition and colorimetric parameters of alcoholic liqueurs prepared from rose petals were evaluated by comparing the potential of three cultivars ('Amadeus', 'Colossal Meidiland' and 'Rosanna') and three traditional methods of preparation (fresh/air-dried petals extracted in 50% ethanol or aqueous sucrose syrup).
30309566	1	51	dep	cultivars	209:217	arg1	Rosanna					257:263	Rosanna	257:263	Rosanna	257:263	Chemical composition and colorimetric parameters of alcoholic liqueurs prepared from rose petals were evaluated by comparing the potential of three cultivars ('Amadeus', 'Colossal Meidiland' and 'Rosanna') and three traditional methods of preparation (fresh/air-dried petals extracted in 50% ethanol or aqueous sucrose syrup).
30309566	1	51	dep	cultivars	209:217	arg1	petals					329:334	fresh/air-dried petals	313:334	fresh/air-dried petals extracted in 50% ethanol or aqueous sucrose syrup	313:384	Chemical composition and colorimetric parameters of alcoholic liqueurs prepared from rose petals were evaluated by comparing the potential of three cultivars ('Amadeus', 'Colossal Meidiland' and 'Rosanna') and three traditional methods of preparation (fresh/air-dried petals extracted in 50% ethanol or aqueous sucrose syrup).
30309566	1	51	dep	cultivars	209:217	arg1	Meidiland					241:249	Meidiland	241:249	Meidiland	241:249	Chemical composition and colorimetric parameters of alcoholic liqueurs prepared from rose petals were evaluated by comparing the potential of three cultivars ('Amadeus', 'Colossal Meidiland' and 'Rosanna') and three traditional methods of preparation (fresh/air-dried petals extracted in 50% ethanol or aqueous sucrose syrup).
30309566	5	52	theme	extraction	805:814	arg1	yields					816:821	The best extraction yields	796:821	The best extraction yields	796:821	The best extraction yields and optimal colour characteristics were achieved by ethanolic extraction of dry petals followed by fresh petal extraction in ethanol and, finally, extraction in sucrose syrup.
30309566	4	53	theme	anthocyanins	675:686	arg1	concentrations					657:670	The highest concentrations	645:670	The highest concentrations of anthocyanins	645:686	The highest concentrations of anthocyanins were determined in extracts from 'Amadeus' petals, followed by 'Colossal Meidiland' and finally, 'Rosanna'.
30309566	5	54	from	extraction	970:979	arg1	ethanol					948:954	ethanol	948:954	ethanol	948:954	The best extraction yields and optimal colour characteristics were achieved by ethanolic extraction of dry petals followed by fresh petal extraction in ethanol and, finally, extraction in sucrose syrup.
30309566	5	54	from	extraction	970:979	arg1	syrup					992:996	sucrose syrup	984:996	sucrose syrup	984:996	The best extraction yields and optimal colour characteristics were achieved by ethanolic extraction of dry petals followed by fresh petal extraction in ethanol and, finally, extraction in sucrose syrup.
30309566	6	55	theme	Amadeus	1019:1025	arg1	petals					1028:1033	'Amadeus' petals	1018:1033	'Amadeus' petals prior to extraction	1018:1053	Air-dehydration of 'Amadeus' petals prior to extraction in 50% ethanol yielded rose liqueur with the best all round characteristics.
30309566	5	56	theme	petal	928:932	arg1	extraction					934:943	fresh petal extraction	922:943	fresh petal extraction in ethanol	922:954	The best extraction yields and optimal colour characteristics were achieved by ethanolic extraction of dry petals followed by fresh petal extraction in ethanol and, finally, extraction in sucrose syrup.
30309566	3	57	theme	chromatography/mass	470:488	arg1	spectrometry					490:501	High performance liquid chromatography/mass spectrometry	446:501	High performance liquid chromatography/mass spectrometry	446:501	High performance liquid chromatography/mass spectrometry was used to confirm the presence of 6 anthocyanins, 4 flavanols, 4 phenolic acids, 2 hydrolysable tannins and 31 flavonols in petal liqueurs.
30309566	1	58	theme	traditional	277:287	arg1	methods					289:295	three traditional methods	271:295	three traditional methods of preparation	271:310	Chemical composition and colorimetric parameters of alcoholic liqueurs prepared from rose petals were evaluated by comparing the potential of three cultivars ('Amadeus', 'Colossal Meidiland' and 'Rosanna') and three traditional methods of preparation (fresh/air-dried petals extracted in 50% ethanol or aqueous sucrose syrup).
30309566	1	59	theme	rose	146:149	arg1	petals					151:156	rose petals	146:156	rose petals	146:156	Chemical composition and colorimetric parameters of alcoholic liqueurs prepared from rose petals were evaluated by comparing the potential of three cultivars ('Amadeus', 'Colossal Meidiland' and 'Rosanna') and three traditional methods of preparation (fresh/air-dried petals extracted in 50% ethanol or aqueous sucrose syrup).
30309566	4	60	theme	highest	649:655	arg1	concentrations					657:670	The highest concentrations	645:670	The highest concentrations of anthocyanins	645:686	The highest concentrations of anthocyanins were determined in extracts from 'Amadeus' petals, followed by 'Colossal Meidiland' and finally, 'Rosanna'.
31152839	10	0	theme	PCL/Zein/GA	1587:1597	arg1	scaffold					1599:1606	The C. officinalis-loaded PCL/Zein/GA scaffold	1561:1606	The C. officinalis-loaded PCL/Zein/GA scaffold	1561:1606	The C. officinalis-loaded PCL/Zein/GA scaffold indicated better antibacterial properties and biocompatibility than PCL/Zein/GA scaffold.
31152839	10	1	theme	C.	1565:1566	arg1	scaffold					1599:1606	The C. officinalis-loaded PCL/Zein/GA scaffold	1561:1606	The C. officinalis-loaded PCL/Zein/GA scaffold	1561:1606	The C. officinalis-loaded PCL/Zein/GA scaffold indicated better antibacterial properties and biocompatibility than PCL/Zein/GA scaffold.
31152839	8	2	theme	multilayer	1302:1311	arg1	electrospinning					1313:1327	multilayer electrospinning	1302:1327	multilayer electrospinning	1302:1327	In vitro C.officinalis release exposed gradual and sustained release behavior for fabricated scaffold by multilayer electrospinning.
31152839	10	3	theme	antibacterial	1625:1637	arg1	properties					1639:1648	better antibacterial properties	1618:1648	better antibacterial properties	1618:1648	The C. officinalis-loaded PCL/Zein/GA scaffold indicated better antibacterial properties and biocompatibility than PCL/Zein/GA scaffold.
31152839	9	4	theme	analysis	1349:1356	arg1	images					1366:1371	MTT analysis and SEM images	1345:1371	MTT analysis and SEM images	1345:1371	The results of MTT analysis and SEM images confirmed that PCL/Zein/GA/C.officinalis nanocomposite scaffold had favorable proliferation and adhesion against fibroblast cell as compared to PCL/Zein/GA scaffold for regenerating skin.
31152839	1	5	theme	officinalis	231:241	arg1	extract					260:266	the Calendula officinalis (C. officinalis) extract	217:266	the Calendula officinalis (C. officinalis) extract	217:266	This work investigates the incorporation of the Calendula officinalis (C. officinalis) extract in electrospun fiber scaffolds composed of poly (ε-caprolactone) (PCL), Zein and gum arabic (GA).
31152839	2	6	theme	hybrid	555:560	arg1	PCL/Zein/GA					562:572	hybrid PCL/Zein/GA	555:572	hybrid PCL/Zein/GA	555:572	Three methods of electrospinning were used: suspension electrospinning, in which C. officinalis extract was directly added in the PCL/Zein/GA solution, two-nozzle electrospinning, in which hybrid PCL/Zein/GA and PCL/C.
31152839	11	7	theme	desirable	1792:1800	arg1	biomaterial					1802:1812	desirable biomaterial	1792:1812	desirable biomaterial for skin regeneration	1792:1834	The results confirmed that C. officinalis-loaded PCL/Zein/GA nanocomposite scaffolds would be desirable biomaterial for skin regeneration.
31152839	0	8	theme	tissue	153:158	arg1	engineering					160:170	skin tissue engineering	148:170	skin tissue engineering	148:170	Calendula officinalis extract/PCL/Zein/Gum arabic nanofibrous bio-composite scaffolds via suspension, two-nozzle and multilayer electrospinning for skin tissue engineering.
31152839	9	9	theme	SEM	1362:1364	arg1	images					1366:1371	MTT analysis and SEM images	1345:1371	MTT analysis and SEM images	1345:1371	The results of MTT analysis and SEM images confirmed that PCL/Zein/GA/C.officinalis nanocomposite scaffold had favorable proliferation and adhesion against fibroblast cell as compared to PCL/Zein/GA scaffold for regenerating skin.
31152839	1	10	theme	C.	244:245	arg1	extract					260:266	the Calendula officinalis (C. officinalis) extract	217:266	the Calendula officinalis (C. officinalis) extract	217:266	This work investigates the incorporation of the Calendula officinalis (C. officinalis) extract in electrospun fiber scaffolds composed of poly (ε-caprolactone) (PCL), Zein and gum arabic (GA).
31152839	6	11	theme	suitable	1031:1038	arg1	degradability					1040:1052	suitable degradability	1031:1052	suitable degradability for skin tissue engineering	1031:1080	The PCL/Zein/GA/C.officinalis scaffolds possess good hydrophilicity with high porosity (about 80%) and also exhibited desirable mechanical properties and suitable degradability for skin tissue engineering.
31152839	7	12	theme	officinalis-loaded	1155:1172	arg1	scaffolds					1186:1194	other C. officinalis-loaded PCL/Zein/GA scaffolds	1146:1194	other C. officinalis-loaded PCL/Zein/GA scaffolds	1146:1194	Multilayer produced scaffold showed more tensile strength than other C. officinalis-loaded PCL/Zein/GA scaffolds.
31152839	7	13	theme	other	1146:1150	arg1	scaffolds					1186:1194	other C. officinalis-loaded PCL/Zein/GA scaffolds	1146:1194	other C. officinalis-loaded PCL/Zein/GA scaffolds	1146:1194	Multilayer produced scaffold showed more tensile strength than other C. officinalis-loaded PCL/Zein/GA scaffolds.
31152839	9	14	theme	PCL/Zein/GA/C.officinalis	1388:1412	arg1	scaffold					1428:1435	PCL/Zein/GA/C.officinalis nanocomposite scaffold	1388:1435	PCL/Zein/GA/C.officinalis nanocomposite scaffold	1388:1435	The results of MTT analysis and SEM images confirmed that PCL/Zein/GA/C.officinalis nanocomposite scaffold had favorable proliferation and adhesion against fibroblast cell as compared to PCL/Zein/GA scaffold for regenerating skin.
31152839	3	15	theme	nanofibrous	597:607	arg1	layers					609:614	officinalis nanofibrous layers	585:614	officinalis nanofibrous layers	585:614	officinalis nanofibrous layers were prepared by two syringes and multilayer electrospinning, in which layer-by-layer scaffold was fabricated of PCL/Zein/GA and PCL/C.
31152839	7	16	theme	produced	1094:1101	arg1	scaffold					1103:1110	Multilayer produced scaffold	1083:1110	Multilayer produced scaffold	1083:1110	Multilayer produced scaffold showed more tensile strength than other C. officinalis-loaded PCL/Zein/GA scaffolds.
31152839	6	17	theme	desirable	995:1003	arg1	properties					1016:1025	desirable mechanical properties	995:1025	desirable mechanical properties	995:1025	The PCL/Zein/GA/C.officinalis scaffolds possess good hydrophilicity with high porosity (about 80%) and also exhibited desirable mechanical properties and suitable degradability for skin tissue engineering.
31152839	4	18	theme	officinalis	751:761	arg1	nanofibrous					763:773	officinalis nanofibrous	751:773	officinalis nanofibrous	751:773	officinalis nanofibrous mats.
31152839	11	19	theme	C.	1725:1726	arg1	scaffolds					1773:1781	C. officinalis-loaded PCL/Zein/GA nanocomposite scaffolds	1725:1781	C. officinalis-loaded PCL/Zein/GA nanocomposite scaffolds	1725:1781	The results confirmed that C. officinalis-loaded PCL/Zein/GA nanocomposite scaffolds would be desirable biomaterial for skin regeneration.
31152839	2	20	theme	PCL/Zein/GA	496:506	arg1	solution					508:515	the PCL/Zein/GA solution	492:515	the PCL/Zein/GA solution	492:515	Three methods of electrospinning were used: suspension electrospinning, in which C. officinalis extract was directly added in the PCL/Zein/GA solution, two-nozzle electrospinning, in which hybrid PCL/Zein/GA and PCL/C.
31152839	2	20	theme	PCL/Zein/GA	496:506	arg1	electrospinning					529:543	two-nozzle electrospinning	518:543	two-nozzle electrospinning	518:543	Three methods of electrospinning were used: suspension electrospinning, in which C. officinalis extract was directly added in the PCL/Zein/GA solution, two-nozzle electrospinning, in which hybrid PCL/Zein/GA and PCL/C.
31152839	9	21	theme	favorable	1441:1449	arg1	proliferation					1451:1463	favorable proliferation	1441:1463	favorable proliferation	1441:1463	The results of MTT analysis and SEM images confirmed that PCL/Zein/GA/C.officinalis nanocomposite scaffold had favorable proliferation and adhesion against fibroblast cell as compared to PCL/Zein/GA scaffold for regenerating skin.
31152839	6	22	contain	possess	917:923	arg1	scaffolds					907:915	The PCL/Zein/GA/C.officinalis scaffolds	877:915	The PCL/Zein/GA/C.officinalis scaffolds	877:915	The PCL/Zein/GA/C.officinalis scaffolds possess good hydrophilicity with high porosity (about 80%) and also exhibited desirable mechanical properties and suitable degradability for skin tissue engineering.
31152839	6	22	contain	possess	917:923	arg2	hydrophilicity					930:943	good hydrophilicity	925:943	good hydrophilicity	925:943	The PCL/Zein/GA/C.officinalis scaffolds possess good hydrophilicity with high porosity (about 80%) and also exhibited desirable mechanical properties and suitable degradability for skin tissue engineering.
31152839	5	23	theme	scaffolds	811:819	arg1	micrographs					785:795	SEM micrographs	781:795	SEM micrographs of fabricated scaffolds	781:819	SEM micrographs of fabricated scaffolds depicted beadless nanofibers with interconnected pores.
31152839	11	24	theme	PCL/Zein/GA	1747:1757	arg1	scaffolds					1773:1781	C. officinalis-loaded PCL/Zein/GA nanocomposite scaffolds	1725:1781	C. officinalis-loaded PCL/Zein/GA nanocomposite scaffolds	1725:1781	The results confirmed that C. officinalis-loaded PCL/Zein/GA nanocomposite scaffolds would be desirable biomaterial for skin regeneration.
31152839	0	25	theme	multilayer	117:126	arg1	electrospinning					128:142	multilayer electrospinning	117:142	multilayer electrospinning	117:142	Calendula officinalis extract/PCL/Zein/Gum arabic nanofibrous bio-composite scaffolds via suspension, two-nozzle and multilayer electrospinning for skin tissue engineering.
31152839	6	26	theme	tissue	1063:1068	arg1	engineering					1070:1080	skin tissue engineering	1058:1080	skin tissue engineering	1058:1080	The PCL/Zein/GA/C.officinalis scaffolds possess good hydrophilicity with high porosity (about 80%) and also exhibited desirable mechanical properties and suitable degradability for skin tissue engineering.
31152839	1	27	dep	Zein	340:343	arg1	arabic					353:358	arabic	353:358	arabic	353:358	This work investigates the incorporation of the Calendula officinalis (C. officinalis) extract in electrospun fiber scaffolds composed of poly (ε-caprolactone) (PCL), Zein and gum arabic (GA).
31152839	5	28	theme	SEM	781:783	arg1	micrographs					785:795	SEM micrographs	781:795	SEM micrographs of fabricated scaffolds	781:819	SEM micrographs of fabricated scaffolds depicted beadless nanofibers with interconnected pores.
31152839	2	29	theme	C.	447:448	arg1	extract					462:468	C. officinalis extract	447:468	C. officinalis extract	447:468	Three methods of electrospinning were used: suspension electrospinning, in which C. officinalis extract was directly added in the PCL/Zein/GA solution, two-nozzle electrospinning, in which hybrid PCL/Zein/GA and PCL/C.
31152839	0	30	theme	Calendula	0:8	arg1	bio-composite					62:74	Calendula officinalis extract/PCL/Zein/Gum arabic nanofibrous bio-composite	0:74	Calendula officinalis extract/PCL/Zein/Gum arabic nanofibrous bio-composite	0:74	Calendula officinalis extract/PCL/Zein/Gum arabic nanofibrous bio-composite scaffolds via suspension, two-nozzle and multilayer electrospinning for skin tissue engineering.
31152839	0	31	theme	extract/PCL/Zein/Gum	22:41	arg1	bio-composite					62:74	Calendula officinalis extract/PCL/Zein/Gum arabic nanofibrous bio-composite	0:74	Calendula officinalis extract/PCL/Zein/Gum arabic nanofibrous bio-composite	0:74	Calendula officinalis extract/PCL/Zein/Gum arabic nanofibrous bio-composite scaffolds via suspension, two-nozzle and multilayer electrospinning for skin tissue engineering.
31152839	9	32	theme	PCL/Zein/GA	1517:1527	arg1	scaffold					1529:1536	PCL/Zein/GA scaffold	1517:1536	PCL/Zein/GA scaffold for regenerating skin	1517:1558	The results of MTT analysis and SEM images confirmed that PCL/Zein/GA/C.officinalis nanocomposite scaffold had favorable proliferation and adhesion against fibroblast cell as compared to PCL/Zein/GA scaffold for regenerating skin.
31152839	0	33	theme	nanofibrous	50:60	arg1	bio-composite					62:74	Calendula officinalis extract/PCL/Zein/Gum arabic nanofibrous bio-composite	0:74	Calendula officinalis extract/PCL/Zein/Gum arabic nanofibrous bio-composite	0:74	Calendula officinalis extract/PCL/Zein/Gum arabic nanofibrous bio-composite scaffolds via suspension, two-nozzle and multilayer electrospinning for skin tissue engineering.
31152839	5	34	theme	beadless	830:837	arg1	nanofibers					839:848	beadless nanofibers	830:848	beadless nanofibers	830:848	SEM micrographs of fabricated scaffolds depicted beadless nanofibers with interconnected pores.
31152839	3	35	theme	multilayer	650:659	arg1	electrospinning					661:675	multilayer electrospinning	650:675	multilayer electrospinning	650:675	officinalis nanofibrous layers were prepared by two syringes and multilayer electrospinning, in which layer-by-layer scaffold was fabricated of PCL/Zein/GA and PCL/C.
31152839	2	36	dep	added	483:487	arg1	PCL/C					578:582	PCL/C	578:582	PCL/C	578:582	Three methods of electrospinning were used: suspension electrospinning, in which C. officinalis extract was directly added in the PCL/Zein/GA solution, two-nozzle electrospinning, in which hybrid PCL/Zein/GA and PCL/C.
31152839	2	36	dep	added	483:487	arg1	PCL/Zein/GA					562:572	hybrid PCL/Zein/GA	555:572	hybrid PCL/Zein/GA	555:572	Three methods of electrospinning were used: suspension electrospinning, in which C. officinalis extract was directly added in the PCL/Zein/GA solution, two-nozzle electrospinning, in which hybrid PCL/Zein/GA and PCL/C.
31152839	6	37	theme	good	925:928	arg1	hydrophilicity					930:943	good hydrophilicity	925:943	good hydrophilicity	925:943	The PCL/Zein/GA/C.officinalis scaffolds possess good hydrophilicity with high porosity (about 80%) and also exhibited desirable mechanical properties and suitable degradability for skin tissue engineering.
31152839	1	38	theme	extract	260:266	arg1	incorporation					200:212	the incorporation	196:212	the incorporation of the Calendula officinalis (C. officinalis) extract in electrospun fiber scaffolds composed of poly (ε-caprolactone) (PCL), Zein and gum arabic (GA)	196:363	This work investigates the incorporation of the Calendula officinalis (C. officinalis) extract in electrospun fiber scaffolds composed of poly (ε-caprolactone) (PCL), Zein and gum arabic (GA).
31152839	8	39	theme	gradual	1236:1242	arg1	behavior					1266:1273	gradual and sustained release behavior	1236:1273	gradual and sustained release behavior	1236:1273	In vitro C.officinalis release exposed gradual and sustained release behavior for fabricated scaffold by multilayer electrospinning.
31152839	9	40	contain	had	1437:1439	arg2	adhesion					1469:1476	adhesion	1469:1476	adhesion	1469:1476	The results of MTT analysis and SEM images confirmed that PCL/Zein/GA/C.officinalis nanocomposite scaffold had favorable proliferation and adhesion against fibroblast cell as compared to PCL/Zein/GA scaffold for regenerating skin.
31152839	9	40	contain	had	1437:1439	arg1	scaffold					1428:1435	PCL/Zein/GA/C.officinalis nanocomposite scaffold	1388:1435	PCL/Zein/GA/C.officinalis nanocomposite scaffold	1388:1435	The results of MTT analysis and SEM images confirmed that PCL/Zein/GA/C.officinalis nanocomposite scaffold had favorable proliferation and adhesion against fibroblast cell as compared to PCL/Zein/GA scaffold for regenerating skin.
31152839	9	40	contain	had	1437:1439	arg2	proliferation					1451:1463	favorable proliferation	1441:1463	favorable proliferation	1441:1463	The results of MTT analysis and SEM images confirmed that PCL/Zein/GA/C.officinalis nanocomposite scaffold had favorable proliferation and adhesion against fibroblast cell as compared to PCL/Zein/GA scaffold for regenerating skin.
31152839	1	41	theme	electrospun	271:281	arg1	scaffolds					289:297	electrospun fiber scaffolds	271:297	electrospun fiber scaffolds composed of poly (ε-caprolactone) (PCL), Zein and gum arabic (GA)	271:363	This work investigates the incorporation of the Calendula officinalis (C. officinalis) extract in electrospun fiber scaffolds composed of poly (ε-caprolactone) (PCL), Zein and gum arabic (GA).
31152839	8	42	theme	sustained	1248:1256	arg1	behavior					1266:1273	gradual and sustained release behavior	1236:1273	gradual and sustained release behavior	1236:1273	In vitro C.officinalis release exposed gradual and sustained release behavior for fabricated scaffold by multilayer electrospinning.
31152839	2	43	from	PCL/Zein/GA	562:572	arg1	which					549:553	which	549:553	which	549:553	Three methods of electrospinning were used: suspension electrospinning, in which C. officinalis extract was directly added in the PCL/Zein/GA solution, two-nozzle electrospinning, in which hybrid PCL/Zein/GA and PCL/C.
31152839	10	44	theme	better	1618:1623	arg1	properties					1639:1648	better antibacterial properties	1618:1648	better antibacterial properties	1618:1648	The C. officinalis-loaded PCL/Zein/GA scaffold indicated better antibacterial properties and biocompatibility than PCL/Zein/GA scaffold.
31152839	10	45	theme	officinalis-loaded	1568:1585	arg1	scaffold					1599:1606	The C. officinalis-loaded PCL/Zein/GA scaffold	1561:1606	The C. officinalis-loaded PCL/Zein/GA scaffold	1561:1606	The C. officinalis-loaded PCL/Zein/GA scaffold indicated better antibacterial properties and biocompatibility than PCL/Zein/GA scaffold.
31152839	8	46	theme	fabricated	1279:1288	arg1	scaffold					1290:1297	fabricated scaffold	1279:1297	fabricated scaffold by multilayer electrospinning	1279:1327	In vitro C.officinalis release exposed gradual and sustained release behavior for fabricated scaffold by multilayer electrospinning.
31152839	2	47	from	PCL/C	578:582	arg1	which					549:553	which	549:553	which	549:553	Three methods of electrospinning were used: suspension electrospinning, in which C. officinalis extract was directly added in the PCL/Zein/GA solution, two-nozzle electrospinning, in which hybrid PCL/Zein/GA and PCL/C.
31152839	9	48	theme	MTT	1345:1347	arg1	analysis					1349:1356	MTT analysis	1345:1356	MTT analysis	1345:1356	The results of MTT analysis and SEM images confirmed that PCL/Zein/GA/C.officinalis nanocomposite scaffold had favorable proliferation and adhesion against fibroblast cell as compared to PCL/Zein/GA scaffold for regenerating skin.
31152839	1	49	theme	Calendula	221:229	arg1	extract					260:266	the Calendula officinalis (C. officinalis) extract	217:266	the Calendula officinalis (C. officinalis) extract	217:266	This work investigates the incorporation of the Calendula officinalis (C. officinalis) extract in electrospun fiber scaffolds composed of poly (ε-caprolactone) (PCL), Zein and gum arabic (GA).
31152839	6	50	theme	skin	1058:1061	arg1	engineering					1070:1080	skin tissue engineering	1058:1080	skin tissue engineering	1058:1080	The PCL/Zein/GA/C.officinalis scaffolds possess good hydrophilicity with high porosity (about 80%) and also exhibited desirable mechanical properties and suitable degradability for skin tissue engineering.
31152839	7	51	theme	PCL/Zein/GA	1174:1184	arg1	scaffolds					1186:1194	other C. officinalis-loaded PCL/Zein/GA scaffolds	1146:1194	other C. officinalis-loaded PCL/Zein/GA scaffolds	1146:1194	Multilayer produced scaffold showed more tensile strength than other C. officinalis-loaded PCL/Zein/GA scaffolds.
31152839	11	52	theme	skin	1818:1821	arg1	regeneration					1823:1834	skin regeneration	1818:1834	skin regeneration	1818:1834	The results confirmed that C. officinalis-loaded PCL/Zein/GA nanocomposite scaffolds would be desirable biomaterial for skin regeneration.
31152839	7	53	theme	C.	1152:1153	arg1	scaffolds					1186:1194	other C. officinalis-loaded PCL/Zein/GA scaffolds	1146:1194	other C. officinalis-loaded PCL/Zein/GA scaffolds	1146:1194	Multilayer produced scaffold showed more tensile strength than other C. officinalis-loaded PCL/Zein/GA scaffolds.
31152839	9	54	theme	images	1366:1371	arg1	results					1334:1340	The results	1330:1340	The results of MTT analysis and SEM images	1330:1371	The results of MTT analysis and SEM images confirmed that PCL/Zein/GA/C.officinalis nanocomposite scaffold had favorable proliferation and adhesion against fibroblast cell as compared to PCL/Zein/GA scaffold for regenerating skin.
31152839	1	55	theme	officinalis	247:257	arg1	extract					260:266	the Calendula officinalis (C. officinalis) extract	217:266	the Calendula officinalis (C. officinalis) extract	217:266	This work investigates the incorporation of the Calendula officinalis (C. officinalis) extract in electrospun fiber scaffolds composed of poly (ε-caprolactone) (PCL), Zein and gum arabic (GA).
31152839	2	56	used	used	404:407	arg2	methods					372:378	Three methods	366:378	Three methods of electrospinning	366:397	Three methods of electrospinning were used: suspension electrospinning, in which C. officinalis extract was directly added in the PCL/Zein/GA solution, two-nozzle electrospinning, in which hybrid PCL/Zein/GA and PCL/C.
31152839	8	57	dep	In	1197:1198	arg1	vitro					1200:1204	vitro	1200:1204	vitro	1200:1204	In vitro C.officinalis release exposed gradual and sustained release behavior for fabricated scaffold by multilayer electrospinning.
31152839	3	58	theme	officinalis	585:595	arg1	layers					609:614	officinalis nanofibrous layers	585:614	officinalis nanofibrous layers	585:614	officinalis nanofibrous layers were prepared by two syringes and multilayer electrospinning, in which layer-by-layer scaffold was fabricated of PCL/Zein/GA and PCL/C.
31152839	7	59	theme	more	1119:1122	arg1	strength					1132:1139	more tensile strength	1119:1139	more tensile strength	1119:1139	Multilayer produced scaffold showed more tensile strength than other C. officinalis-loaded PCL/Zein/GA scaffolds.
31152839	6	60	theme	mechanical	1005:1014	arg1	properties					1016:1025	desirable mechanical properties	995:1025	desirable mechanical properties	995:1025	The PCL/Zein/GA/C.officinalis scaffolds possess good hydrophilicity with high porosity (about 80%) and also exhibited desirable mechanical properties and suitable degradability for skin tissue engineering.
31152839	7	61	theme	tensile	1124:1130	arg1	strength					1132:1139	more tensile strength	1119:1139	more tensile strength	1119:1139	Multilayer produced scaffold showed more tensile strength than other C. officinalis-loaded PCL/Zein/GA scaffolds.
31152839	9	62	theme	nanocomposite	1414:1426	arg1	scaffold					1428:1435	PCL/Zein/GA/C.officinalis nanocomposite scaffold	1388:1435	PCL/Zein/GA/C.officinalis nanocomposite scaffold	1388:1435	The results of MTT analysis and SEM images confirmed that PCL/Zein/GA/C.officinalis nanocomposite scaffold had favorable proliferation and adhesion against fibroblast cell as compared to PCL/Zein/GA scaffold for regenerating skin.
31152839	7	63	theme	Multilayer	1083:1092	arg1	scaffold					1103:1110	Multilayer produced scaffold	1083:1110	Multilayer produced scaffold	1083:1110	Multilayer produced scaffold showed more tensile strength than other C. officinalis-loaded PCL/Zein/GA scaffolds.
31152839	11	64	theme	nanocomposite	1759:1771	arg1	scaffolds					1773:1781	C. officinalis-loaded PCL/Zein/GA nanocomposite scaffolds	1725:1781	C. officinalis-loaded PCL/Zein/GA nanocomposite scaffolds	1725:1781	The results confirmed that C. officinalis-loaded PCL/Zein/GA nanocomposite scaffolds would be desirable biomaterial for skin regeneration.
31152839	5	65	theme	fabricated	800:809	arg1	scaffolds					811:819	fabricated scaffolds	800:819	fabricated scaffolds	800:819	SEM micrographs of fabricated scaffolds depicted beadless nanofibers with interconnected pores.
31152839	11	66	theme	officinalis-loaded	1728:1745	arg1	scaffolds					1773:1781	C. officinalis-loaded PCL/Zein/GA nanocomposite scaffolds	1725:1781	C. officinalis-loaded PCL/Zein/GA nanocomposite scaffolds	1725:1781	The results confirmed that C. officinalis-loaded PCL/Zein/GA nanocomposite scaffolds would be desirable biomaterial for skin regeneration.
31152839	1	67	from	incorporation	200:212	arg1	scaffolds					289:297	electrospun fiber scaffolds	271:297	electrospun fiber scaffolds composed of poly (ε-caprolactone) (PCL), Zein and gum arabic (GA)	271:363	This work investigates the incorporation of the Calendula officinalis (C. officinalis) extract in electrospun fiber scaffolds composed of poly (ε-caprolactone) (PCL), Zein and gum arabic (GA).
31152839	2	68	theme	two-nozzle	518:527	arg1	solution					508:515	the PCL/Zein/GA solution	492:515	the PCL/Zein/GA solution	492:515	Three methods of electrospinning were used: suspension electrospinning, in which C. officinalis extract was directly added in the PCL/Zein/GA solution, two-nozzle electrospinning, in which hybrid PCL/Zein/GA and PCL/C.
31152839	2	68	theme	two-nozzle	518:527	arg1	electrospinning					529:543	two-nozzle electrospinning	518:543	two-nozzle electrospinning	518:543	Three methods of electrospinning were used: suspension electrospinning, in which C. officinalis extract was directly added in the PCL/Zein/GA solution, two-nozzle electrospinning, in which hybrid PCL/Zein/GA and PCL/C.
31152839	0	69	theme	skin	148:151	arg1	engineering					160:170	skin tissue engineering	148:170	skin tissue engineering	148:170	Calendula officinalis extract/PCL/Zein/Gum arabic nanofibrous bio-composite scaffolds via suspension, two-nozzle and multilayer electrospinning for skin tissue engineering.
31152839	9	70	theme	fibroblast	1486:1495	arg1	cell					1497:1500	fibroblast cell	1486:1500	fibroblast cell	1486:1500	The results of MTT analysis and SEM images confirmed that PCL/Zein/GA/C.officinalis nanocomposite scaffold had favorable proliferation and adhesion against fibroblast cell as compared to PCL/Zein/GA scaffold for regenerating skin.
31152839	2	71	theme	electrospinning	383:397	arg1	methods					372:378	Three methods	366:378	Three methods of electrospinning	366:397	Three methods of electrospinning were used: suspension electrospinning, in which C. officinalis extract was directly added in the PCL/Zein/GA solution, two-nozzle electrospinning, in which hybrid PCL/Zein/GA and PCL/C.
31152839	5	72	theme	interconnected	855:868	arg1	pores					870:874	interconnected pores	855:874	interconnected pores	855:874	SEM micrographs of fabricated scaffolds depicted beadless nanofibers with interconnected pores.
31152839	10	73	theme	PCL/Zein/GA	1676:1686	arg1	scaffold					1688:1695	PCL/Zein/GA scaffold	1676:1695	PCL/Zein/GA scaffold	1676:1695	The C. officinalis-loaded PCL/Zein/GA scaffold indicated better antibacterial properties and biocompatibility than PCL/Zein/GA scaffold.
31152839	0	74	theme	arabic	43:48	arg1	bio-composite					62:74	Calendula officinalis extract/PCL/Zein/Gum arabic nanofibrous bio-composite	0:74	Calendula officinalis extract/PCL/Zein/Gum arabic nanofibrous bio-composite	0:74	Calendula officinalis extract/PCL/Zein/Gum arabic nanofibrous bio-composite scaffolds via suspension, two-nozzle and multilayer electrospinning for skin tissue engineering.
31152839	8	75	theme	In	1197:1198	arg1	release					1220:1226	In vitro C.officinalis release	1197:1226	In vitro C.officinalis release	1197:1226	In vitro C.officinalis release exposed gradual and sustained release behavior for fabricated scaffold by multilayer electrospinning.
31152839	2	76	theme	suspension	410:419	arg1	electrospinning					421:435	suspension electrospinning	410:435	suspension electrospinning	410:435	Three methods of electrospinning were used: suspension electrospinning, in which C. officinalis extract was directly added in the PCL/Zein/GA solution, two-nozzle electrospinning, in which hybrid PCL/Zein/GA and PCL/C.
31152839	8	77	theme	C.officinalis	1206:1218	arg1	release					1220:1226	In vitro C.officinalis release	1197:1226	In vitro C.officinalis release	1197:1226	In vitro C.officinalis release exposed gradual and sustained release behavior for fabricated scaffold by multilayer electrospinning.
31152839	6	78	theme	high	950:953	arg1	%					973:973	about 80%	965:973	about 80%	965:973	The PCL/Zein/GA/C.officinalis scaffolds possess good hydrophilicity with high porosity (about 80%) and also exhibited desirable mechanical properties and suitable degradability for skin tissue engineering.
31152839	6	78	theme	high	950:953	arg1	porosity					955:962	high porosity	950:962	high porosity (about 80%)	950:974	The PCL/Zein/GA/C.officinalis scaffolds possess good hydrophilicity with high porosity (about 80%) and also exhibited desirable mechanical properties and suitable degradability for skin tissue engineering.
31152839	9	79	theme	regenerating	1542:1553	arg1	skin					1555:1558	regenerating skin	1542:1558	regenerating skin	1542:1558	The results of MTT analysis and SEM images confirmed that PCL/Zein/GA/C.officinalis nanocomposite scaffold had favorable proliferation and adhesion against fibroblast cell as compared to PCL/Zein/GA scaffold for regenerating skin.
31152839	2	80	theme	officinalis	450:460	arg1	extract					462:468	C. officinalis extract	447:468	C. officinalis extract	447:468	Three methods of electrospinning were used: suspension electrospinning, in which C. officinalis extract was directly added in the PCL/Zein/GA solution, two-nozzle electrospinning, in which hybrid PCL/Zein/GA and PCL/C.
31152839	3	81	theme	layer-by-layer	687:700	arg1	scaffold					702:709	layer-by-layer scaffold	687:709	layer-by-layer scaffold	687:709	officinalis nanofibrous layers were prepared by two syringes and multilayer electrospinning, in which layer-by-layer scaffold was fabricated of PCL/Zein/GA and PCL/C.
31152839	8	82	theme	release	1258:1264	arg1	behavior					1266:1273	gradual and sustained release behavior	1236:1273	gradual and sustained release behavior	1236:1273	In vitro C.officinalis release exposed gradual and sustained release behavior for fabricated scaffold by multilayer electrospinning.
31152839	6	83	theme	PCL/Zein/GA/C.officinalis	881:905	arg1	scaffolds					907:915	The PCL/Zein/GA/C.officinalis scaffolds	877:915	The PCL/Zein/GA/C.officinalis scaffolds	877:915	The PCL/Zein/GA/C.officinalis scaffolds possess good hydrophilicity with high porosity (about 80%) and also exhibited desirable mechanical properties and suitable degradability for skin tissue engineering.
31152839	0	84	theme	officinalis	10:20	arg1	bio-composite					62:74	Calendula officinalis extract/PCL/Zein/Gum arabic nanofibrous bio-composite	0:74	Calendula officinalis extract/PCL/Zein/Gum arabic nanofibrous bio-composite	0:74	Calendula officinalis extract/PCL/Zein/Gum arabic nanofibrous bio-composite scaffolds via suspension, two-nozzle and multilayer electrospinning for skin tissue engineering.
31152839	1	85	theme	fiber	283:287	arg1	scaffolds					289:297	electrospun fiber scaffolds	271:297	electrospun fiber scaffolds composed of poly (ε-caprolactone) (PCL), Zein and gum arabic (GA)	271:363	This work investigates the incorporation of the Calendula officinalis (C. officinalis) extract in electrospun fiber scaffolds composed of poly (ε-caprolactone) (PCL), Zein and gum arabic (GA).
30707309	8	0	theme	Schematic	1052:1060	arg1	presentation					1062:1073	Graphical abstract Schematic presentation	1033:1073	Graphical abstract Schematic presentation of the fabrication of the Rf/AuPt-PPy/GR/GCE surface and the measurement principle by differential pulse voltammetry (DPV).	1033:1197	Graphical abstract Schematic presentation of the fabrication of the Rf/AuPt-PPy/GR/GCE surface and the measurement principle by differential pulse voltammetry (DPV).
30707309	5	1	theme	AuPt-PPy/GR-modified	707:726	arg1	GCE					728:730	the AuPt-PPy/GR-modified GCE	703:730	the AuPt-PPy/GR-modified GCE	703:730	Finally, Rf was electrodeposited on the surface of the AuPt-PPy/GR-modified GCE.
30707309	6	2	theme	concentration	868:880	arg1	range					882:886	the 2.5-600 μM concentration range	853:886	the 2.5-600 μM concentration range	853:886	Under optimized conditions, the GCE provided high sensitivity and selectivity for Sar at around 0.61 V. Response covers the 2.5-600 μM concentration range, and the detection limit is 0.68 μM.
30707309	4	3	mod	modified	444:451	arg3	GR					479:480	GR	479:480	GR	479:480	A glassy carbon electrode (GCE) was modified with a graphene-chitosan (GR) composite and further modified with gold-platinum bimetallic nanoparticles in a polypyrrole (PPy) matrix in order to enhance the catalytic activity of the enzyme mimic.
30707309	4	3	mod	modified	444:451	arg1	GCE					435:437	GCE	435:437	GCE	435:437	A glassy carbon electrode (GCE) was modified with a graphene-chitosan (GR) composite and further modified with gold-platinum bimetallic nanoparticles in a polypyrrole (PPy) matrix in order to enhance the catalytic activity of the enzyme mimic.
30707309	4	3	mod	modified	444:451	arg1	electrode					424:432	A glassy carbon electrode	408:432	A glassy carbon electrode (GCE)	408:438	A glassy carbon electrode (GCE) was modified with a graphene-chitosan (GR) composite and further modified with gold-platinum bimetallic nanoparticles in a polypyrrole (PPy) matrix in order to enhance the catalytic activity of the enzyme mimic.
30707309	4	3	mod	modified	444:451	arg3	graphene-chitosan					460:476	a graphene-chitosan	458:476	a graphene-chitosan (GR) composite and further modified with gold-platinum bimetallic nanoparticles in a polypyrrole (PPy) matrix in order to enhance the catalytic activity of the enzyme mimic	458:649	A glassy carbon electrode (GCE) was modified with a graphene-chitosan (GR) composite and further modified with gold-platinum bimetallic nanoparticles in a polypyrrole (PPy) matrix in order to enhance the catalytic activity of the enzyme mimic.
30707309	4	4	theme	enzyme	638:643	arg1	activity					622:629	the catalytic activity	608:629	the catalytic activity of the enzyme mimic	608:649	A glassy carbon electrode (GCE) was modified with a graphene-chitosan (GR) composite and further modified with gold-platinum bimetallic nanoparticles in a polypyrrole (PPy) matrix in order to enhance the catalytic activity of the enzyme mimic.
30707309	4	5	theme	bimetallic	533:542	arg1	nanoparticles					544:556	gold-platinum bimetallic nanoparticles	519:556	gold-platinum bimetallic nanoparticles	519:556	A glassy carbon electrode (GCE) was modified with a graphene-chitosan (GR) composite and further modified with gold-platinum bimetallic nanoparticles in a polypyrrole (PPy) matrix in order to enhance the catalytic activity of the enzyme mimic.
30707309	8	6	theme	surface	1120:1126	arg1	fabrication					1082:1092	the fabrication	1078:1092	the fabrication of the Rf/AuPt-PPy/GR/GCE surface and the measurement principle	1078:1156	Graphical abstract Schematic presentation of the fabrication of the Rf/AuPt-PPy/GR/GCE surface and the measurement principle by differential pulse voltammetry (DPV).
30707309	6	7	theme	2.5-600 μM	857:866	arg1	range					882:886	the 2.5-600 μM concentration range	853:886	the 2.5-600 μM concentration range	853:886	Under optimized conditions, the GCE provided high sensitivity and selectivity for Sar at around 0.61 V. Response covers the 2.5-600 μM concentration range, and the detection limit is 0.68 μM.
30707309	8	8	theme	Rf/AuPt-PPy/GR/GCE	1101:1118	arg1	surface					1120:1126	the Rf/AuPt-PPy/GR/GCE surface	1097:1126	the Rf/AuPt-PPy/GR/GCE surface	1097:1126	Graphical abstract Schematic presentation of the fabrication of the Rf/AuPt-PPy/GR/GCE surface and the measurement principle by differential pulse voltammetry (DPV).
30707309	4	9	dep	enzyme	638:643	arg1	mimic					645:649	mimic	645:649	mimic	645:649	A glassy carbon electrode (GCE) was modified with a graphene-chitosan (GR) composite and further modified with gold-platinum bimetallic nanoparticles in a polypyrrole (PPy) matrix in order to enhance the catalytic activity of the enzyme mimic.
30707309	1	10	theme	nonenzymatic	71:82	arg1	sensor					100:105	A nonenzymatic electrochemical sensor	69:105	A nonenzymatic electrochemical sensor	69:105	A nonenzymatic electrochemical sensor is described for the prostate cancer biomarker sarcosine (Sar).
30707309	0	11	theme	biomimetic	45:54	arg1	recognition					56:66	biomimetic recognition	45:66	biomimetic recognition	45:66	An electrochemical sarcosine sensor based on biomimetic recognition.
30707309	6	12	theme	optimized	739:747	arg1	conditions					749:758	optimized conditions	739:758	optimized conditions	739:758	Under optimized conditions, the GCE provided high sensitivity and selectivity for Sar at around 0.61 V. Response covers the 2.5-600 μM concentration range, and the detection limit is 0.68 μM.
30707309	1	13	theme	electrochemical	84:98	arg1	sensor					100:105	A nonenzymatic electrochemical sensor	69:105	A nonenzymatic electrochemical sensor	69:105	A nonenzymatic electrochemical sensor is described for the prostate cancer biomarker sarcosine (Sar).
30707309	0	14	theme	electrochemical	3:17	arg1	sensor					29:34	An electrochemical sarcosine sensor	0:34	An electrochemical sarcosine sensor	0:34	An electrochemical sarcosine sensor based on biomimetic recognition.
30707309	6	15	theme	around 0.61 V.	822:835	arg1	Response					837:844	around 0.61 V. Response	822:844	around 0.61 V. Response	822:844	Under optimized conditions, the GCE provided high sensitivity and selectivity for Sar at around 0.61 V. Response covers the 2.5-600 μM concentration range, and the detection limit is 0.68 μM.
30707309	7	16	theme	%	1021:1021	arg1	recovery					1023:1030	98.0%-103.2% recovery	1010:1030	98.0%-103.2% recovery	1010:1030	The method was successfully applied to the determination of Sar in spiked urine with 98.0%-103.2% recovery.
30707309	8	17	theme	abstract	1043:1050	arg1	presentation					1062:1073	Graphical abstract Schematic presentation	1033:1073	Graphical abstract Schematic presentation of the fabrication of the Rf/AuPt-PPy/GR/GCE surface and the measurement principle by differential pulse voltammetry (DPV).	1033:1197	Graphical abstract Schematic presentation of the fabrication of the Rf/AuPt-PPy/GR/GCE surface and the measurement principle by differential pulse voltammetry (DPV).
30707309	3	18	theme	poor	392:395	arg1	stability					397:405	poor stability	392:405	poor stability	392:405	The use of riboflavon (Rf) avoids the disadvantages of an enzymatic sensor, such as high cost and poor stability.
30707309	8	19	theme	measurement	1136:1146	arg1	principle					1148:1156	the measurement principle	1132:1156	the measurement principle	1132:1156	Graphical abstract Schematic presentation of the fabrication of the Rf/AuPt-PPy/GR/GCE surface and the measurement principle by differential pulse voltammetry (DPV).
30707309	4	20	theme	modified	505:512	arg1	GR					479:480	GR	479:480	GR	479:480	A glassy carbon electrode (GCE) was modified with a graphene-chitosan (GR) composite and further modified with gold-platinum bimetallic nanoparticles in a polypyrrole (PPy) matrix in order to enhance the catalytic activity of the enzyme mimic.
30707309	4	20	theme	modified	505:512	arg1	graphene-chitosan					460:476	a graphene-chitosan	458:476	a graphene-chitosan (GR) composite and further modified with gold-platinum bimetallic nanoparticles in a polypyrrole (PPy) matrix in order to enhance the catalytic activity of the enzyme mimic	458:649	A glassy carbon electrode (GCE) was modified with a graphene-chitosan (GR) composite and further modified with gold-platinum bimetallic nanoparticles in a polypyrrole (PPy) matrix in order to enhance the catalytic activity of the enzyme mimic.
30707309	4	21	theme	catalytic	612:620	arg1	activity					622:629	the catalytic activity	608:629	the catalytic activity of the enzyme mimic	608:649	A glassy carbon electrode (GCE) was modified with a graphene-chitosan (GR) composite and further modified with gold-platinum bimetallic nanoparticles in a polypyrrole (PPy) matrix in order to enhance the catalytic activity of the enzyme mimic.
30707309	3	22	theme	enzymatic	352:360	arg1	sensor					362:367	an enzymatic sensor	349:367	an enzymatic sensor	349:367	The use of riboflavon (Rf) avoids the disadvantages of an enzymatic sensor, such as high cost and poor stability.
30707309	7	23	theme	%	1014:1014	arg1	%					1021:1021	98.0%-103.2%	1010:1021	98.0%-103.2% recovery	1010:1030	The method was successfully applied to the determination of Sar in spiked urine with 98.0%-103.2% recovery.
30707309	3	24	theme	sensor	362:367	arg1	disadvantages					332:344	the disadvantages	328:344	the disadvantages	328:344	The use of riboflavon (Rf) avoids the disadvantages of an enzymatic sensor, such as high cost and poor stability.
30707309	3	24	theme	sensor	362:367	arg1	cost					383:386	high cost	378:386	high cost	378:386	The use of riboflavon (Rf) avoids the disadvantages of an enzymatic sensor, such as high cost and poor stability.
30707309	3	24	theme	sensor	362:367	arg1	stability					397:405	poor stability	392:405	poor stability	392:405	The use of riboflavon (Rf) avoids the disadvantages of an enzymatic sensor, such as high cost and poor stability.
30707309	4	25	theme	gold-platinum	519:531	arg1	nanoparticles					544:556	gold-platinum bimetallic nanoparticles	519:556	gold-platinum bimetallic nanoparticles	519:556	A glassy carbon electrode (GCE) was modified with a graphene-chitosan (GR) composite and further modified with gold-platinum bimetallic nanoparticles in a polypyrrole (PPy) matrix in order to enhance the catalytic activity of the enzyme mimic.
30707309	2	26	theme	biomimetic	275:284	arg1	sensor					286:291	the biomimetic sensor	271:291	the biomimetic sensor	271:291	Riboflavin was employed to mimic the active center of the enzyme sarcosine oxidase for constructing the biomimetic sensor.
30707309	4	27	theme	carbon	417:422	arg1	GCE					435:437	GCE	435:437	GCE	435:437	A glassy carbon electrode (GCE) was modified with a graphene-chitosan (GR) composite and further modified with gold-platinum bimetallic nanoparticles in a polypyrrole (PPy) matrix in order to enhance the catalytic activity of the enzyme mimic.
30707309	4	27	theme	carbon	417:422	arg1	electrode					424:432	A glassy carbon electrode	408:432	A glassy carbon electrode (GCE)	408:438	A glassy carbon electrode (GCE) was modified with a graphene-chitosan (GR) composite and further modified with gold-platinum bimetallic nanoparticles in a polypyrrole (PPy) matrix in order to enhance the catalytic activity of the enzyme mimic.
30707309	5	28	theme	GCE	728:730	arg1	surface					692:698	the surface	688:698	the surface of the AuPt-PPy/GR-modified GCE	688:730	Finally, Rf was electrodeposited on the surface of the AuPt-PPy/GR-modified GCE.
30707309	8	29	theme	Graphical	1033:1041	arg1	presentation					1062:1073	Graphical abstract Schematic presentation	1033:1073	Graphical abstract Schematic presentation of the fabrication of the Rf/AuPt-PPy/GR/GCE surface and the measurement principle by differential pulse voltammetry (DPV).	1033:1197	Graphical abstract Schematic presentation of the fabrication of the Rf/AuPt-PPy/GR/GCE surface and the measurement principle by differential pulse voltammetry (DPV).
30707309	4	30	theme	glassy	410:415	arg1	GCE					435:437	GCE	435:437	GCE	435:437	A glassy carbon electrode (GCE) was modified with a graphene-chitosan (GR) composite and further modified with gold-platinum bimetallic nanoparticles in a polypyrrole (PPy) matrix in order to enhance the catalytic activity of the enzyme mimic.
30707309	4	30	theme	glassy	410:415	arg1	electrode					424:432	A glassy carbon electrode	408:432	A glassy carbon electrode (GCE)	408:438	A glassy carbon electrode (GCE) was modified with a graphene-chitosan (GR) composite and further modified with gold-platinum bimetallic nanoparticles in a polypyrrole (PPy) matrix in order to enhance the catalytic activity of the enzyme mimic.
30707309	2	31	theme	enzyme	229:234	arg1	oxidase					246:252	the enzyme sarcosine oxidase	225:252	the enzyme sarcosine oxidase	225:252	Riboflavin was employed to mimic the active center of the enzyme sarcosine oxidase for constructing the biomimetic sensor.
30707309	7	32	with	urine	999:1003	arg1	recovery					1023:1030	98.0%-103.2% recovery	1010:1030	98.0%-103.2% recovery	1010:1030	The method was successfully applied to the determination of Sar in spiked urine with 98.0%-103.2% recovery.
30707309	0	33	theme	sarcosine	19:27	arg1	sensor					29:34	An electrochemical sarcosine sensor	0:34	An electrochemical sarcosine sensor	0:34	An electrochemical sarcosine sensor based on biomimetic recognition.
30707309	8	34	theme	pulse	1174:1178	arg1	voltammetry					1180:1190	differential pulse voltammetry	1161:1190	differential pulse voltammetry (DPV)	1161:1196	Graphical abstract Schematic presentation of the fabrication of the Rf/AuPt-PPy/GR/GCE surface and the measurement principle by differential pulse voltammetry (DPV).
30707309	8	34	theme	pulse	1174:1178	arg1	DPV					1193:1195	DPV	1193:1195	DPV	1193:1195	Graphical abstract Schematic presentation of the fabrication of the Rf/AuPt-PPy/GR/GCE surface and the measurement principle by differential pulse voltammetry (DPV).
30707309	8	35	theme	differential	1161:1172	arg1	voltammetry					1180:1190	differential pulse voltammetry	1161:1190	differential pulse voltammetry (DPV)	1161:1196	Graphical abstract Schematic presentation of the fabrication of the Rf/AuPt-PPy/GR/GCE surface and the measurement principle by differential pulse voltammetry (DPV).
30707309	8	35	theme	differential	1161:1172	arg1	DPV					1193:1195	DPV	1193:1195	DPV	1193:1195	Graphical abstract Schematic presentation of the fabrication of the Rf/AuPt-PPy/GR/GCE surface and the measurement principle by differential pulse voltammetry (DPV).
30707309	6	36	theme	detection	897:905	arg1	0.68 μM					916:922	0.68 μM	916:922	0.68 μM	916:922	Under optimized conditions, the GCE provided high sensitivity and selectivity for Sar at around 0.61 V. Response covers the 2.5-600 μM concentration range, and the detection limit is 0.68 μM.
30707309	6	36	theme	detection	897:905	arg1	limit					907:911	the detection limit	893:911	the detection limit	893:911	Under optimized conditions, the GCE provided high sensitivity and selectivity for Sar at around 0.61 V. Response covers the 2.5-600 μM concentration range, and the detection limit is 0.68 μM.
30707309	7	37	theme	spiked	992:997	arg1	urine					999:1003	spiked urine	992:1003	spiked urine with 98.0%-103.2% recovery	992:1030	The method was successfully applied to the determination of Sar in spiked urine with 98.0%-103.2% recovery.
30707309	3	38	theme	high	378:381	arg1	cost					383:386	high cost	378:386	high cost	378:386	The use of riboflavon (Rf) avoids the disadvantages of an enzymatic sensor, such as high cost and poor stability.
30707309	3	39	theme	riboflavon	305:314	arg1	use					298:300	The use	294:300	The use of riboflavon (Rf)	294:319	The use of riboflavon (Rf) avoids the disadvantages of an enzymatic sensor, such as high cost and poor stability.
30707309	2	40	theme	active	208:213	arg1	center					215:220	the active center	204:220	the active center of the enzyme sarcosine oxidase for constructing the biomimetic sensor	204:291	Riboflavin was employed to mimic the active center of the enzyme sarcosine oxidase for constructing the biomimetic sensor.
30707309	1	41	theme	prostate	128:135	arg1	Sar					165:167	the prostate cancer biomarker sarcosine (Sar)	124:168	the prostate cancer biomarker sarcosine (Sar)	124:168	A nonenzymatic electrochemical sensor is described for the prostate cancer biomarker sarcosine (Sar).
30707309	4	42	theme	composite	483:491	arg1	GR					479:480	GR	479:480	GR	479:480	A glassy carbon electrode (GCE) was modified with a graphene-chitosan (GR) composite and further modified with gold-platinum bimetallic nanoparticles in a polypyrrole (PPy) matrix in order to enhance the catalytic activity of the enzyme mimic.
30707309	4	42	theme	composite	483:491	arg1	graphene-chitosan					460:476	a graphene-chitosan	458:476	a graphene-chitosan (GR) composite and further modified with gold-platinum bimetallic nanoparticles in a polypyrrole (PPy) matrix in order to enhance the catalytic activity of the enzyme mimic	458:649	A glassy carbon electrode (GCE) was modified with a graphene-chitosan (GR) composite and further modified with gold-platinum bimetallic nanoparticles in a polypyrrole (PPy) matrix in order to enhance the catalytic activity of the enzyme mimic.
30707309	7	43	theme	Sar	985:987	arg1	determination					968:980	the determination	964:980	the determination of Sar	964:987	The method was successfully applied to the determination of Sar in spiked urine with 98.0%-103.2% recovery.
30707309	1	44	theme	cancer	137:142	arg1	Sar					165:167	the prostate cancer biomarker sarcosine (Sar)	124:168	the prostate cancer biomarker sarcosine (Sar)	124:168	A nonenzymatic electrochemical sensor is described for the prostate cancer biomarker sarcosine (Sar).
30707309	6	45	theme	high	778:781	arg1	sensitivity					783:793	high sensitivity	778:793	high sensitivity	778:793	Under optimized conditions, the GCE provided high sensitivity and selectivity for Sar at around 0.61 V. Response covers the 2.5-600 μM concentration range, and the detection limit is 0.68 μM.
30707309	1	46	theme	biomarker	144:152	arg1	Sar					165:167	the prostate cancer biomarker sarcosine (Sar)	124:168	the prostate cancer biomarker sarcosine (Sar)	124:168	A nonenzymatic electrochemical sensor is described for the prostate cancer biomarker sarcosine (Sar).
30707309	4	47	theme	polypyrrole	563:573	arg1	matrix					581:586	a polypyrrole (PPy) matrix	561:586	a polypyrrole (PPy) matrix in order to enhance the catalytic activity of the enzyme mimic	561:649	A glassy carbon electrode (GCE) was modified with a graphene-chitosan (GR) composite and further modified with gold-platinum bimetallic nanoparticles in a polypyrrole (PPy) matrix in order to enhance the catalytic activity of the enzyme mimic.
30707309	8	48	theme	fabrication	1082:1092	arg1	presentation					1062:1073	Graphical abstract Schematic presentation	1033:1073	Graphical abstract Schematic presentation of the fabrication of the Rf/AuPt-PPy/GR/GCE surface and the measurement principle by differential pulse voltammetry (DPV).	1033:1197	Graphical abstract Schematic presentation of the fabrication of the Rf/AuPt-PPy/GR/GCE surface and the measurement principle by differential pulse voltammetry (DPV).
30707309	2	49	theme	oxidase	246:252	arg1	center					215:220	the active center	204:220	the active center of the enzyme sarcosine oxidase for constructing the biomimetic sensor	204:291	Riboflavin was employed to mimic the active center of the enzyme sarcosine oxidase for constructing the biomimetic sensor.
30707309	1	50	theme	sarcosine	154:162	arg1	Sar					165:167	the prostate cancer biomarker sarcosine (Sar)	124:168	the prostate cancer biomarker sarcosine (Sar)	124:168	A nonenzymatic electrochemical sensor is described for the prostate cancer biomarker sarcosine (Sar).
30707309	2	51	theme	sarcosine	236:244	arg1	oxidase					246:252	the enzyme sarcosine oxidase	225:252	the enzyme sarcosine oxidase	225:252	Riboflavin was employed to mimic the active center of the enzyme sarcosine oxidase for constructing the biomimetic sensor.
30707309	8	52	theme	principle	1148:1156	arg1	fabrication					1082:1092	the fabrication	1078:1092	the fabrication of the Rf/AuPt-PPy/GR/GCE surface and the measurement principle	1078:1156	Graphical abstract Schematic presentation of the fabrication of the Rf/AuPt-PPy/GR/GCE surface and the measurement principle by differential pulse voltammetry (DPV).
30707309	4	53	theme	PPy	576:578	arg1	matrix					581:586	a polypyrrole (PPy) matrix	561:586	a polypyrrole (PPy) matrix in order to enhance the catalytic activity of the enzyme mimic	561:649	A glassy carbon electrode (GCE) was modified with a graphene-chitosan (GR) composite and further modified with gold-platinum bimetallic nanoparticles in a polypyrrole (PPy) matrix in order to enhance the catalytic activity of the enzyme mimic.
31495347	0	0	theme	intestinal	89:98	arg1	morphology					100:109	intestinal morphology	89:109	intestinal morphology	89:109	Dietary administration of resistant starch improved caecal barrier function by enhancing intestinal morphology and modulating microbiota composition in meat duck.
31495347	1	1	theme	meat	307:310	arg1	ducks					312:316	meat ducks	307:316	meat ducks	307:316	Resistant starch (RS) was recently approved to exert a powerful influence on gut health, but the effect of RS on the caecal barrier function in meat ducks has not been well defined.
31495347	2	2	theme	meat	480:483	arg1	ducks					485:489	meat ducks	480:489	meat ducks	480:489	Thus, the effect of raw potato starch (RPS), a widely adopted RS material, on microbial composition and barrier function of caecum for meat ducks was determined.
31495347	4	3	theme	butyrate-producing	877:894	arg1	Faecalibacterium					905:920	Faecalibacterium	905:920	Faecalibacterium	905:920	The 16S rRNA sequence analysis indicated that the diet with 12 % RPS had a higher relative abundance of Firmicutes and the butyrate-producing bacteria Faecalibacterium, Subdoligranulum, and Erysipelatoclostridium were enriched in all diets.
31495347	4	3	theme	butyrate-producing	877:894	arg1	bacteria					896:903	the butyrate-producing bacteria	873:903	the butyrate-producing bacteria Faecalibacterium, Subdoligranulum, and Erysipelatoclostridium	873:965	The 16S rRNA sequence analysis indicated that the diet with 12 % RPS had a higher relative abundance of Firmicutes and the butyrate-producing bacteria Faecalibacterium, Subdoligranulum, and Erysipelatoclostridium were enriched in all diets.
31495347	4	3	theme	butyrate-producing	877:894	arg1	Subdoligranulum					923:937	Subdoligranulum	923:937	Subdoligranulum	923:937	The 16S rRNA sequence analysis indicated that the diet with 12 % RPS had a higher relative abundance of Firmicutes and the butyrate-producing bacteria Faecalibacterium, Subdoligranulum, and Erysipelatoclostridium were enriched in all diets.
31495347	4	3	theme	butyrate-producing	877:894	arg1	Erysipelatoclostridium					944:965	Erysipelatoclostridium	944:965	Erysipelatoclostridium	944:965	The 16S rRNA sequence analysis indicated that the diet with 12 % RPS had a higher relative abundance of Firmicutes and the butyrate-producing bacteria Faecalibacterium, Subdoligranulum, and Erysipelatoclostridium were enriched in all diets.
31495347	5	4	theme	RPS	1070:1072	arg1	diet					1074:1077	the 24 % RPS diet	1061:1077	the 24 % RPS diet v. the control diet	1061:1097	Lactobacillus and Bifidobacterium were significantly increased in the 24 % RPS diet v. the control diet.
31495347	2	5	theme	RS	407:408	arg1	starch					376:381	raw potato starch	365:381	raw potato starch (RPS)	365:387	Thus, the effect of raw potato starch (RPS), a widely adopted RS material, on microbial composition and barrier function of caecum for meat ducks was determined.
31495347	2	5	theme	RS	407:408	arg1	material					410:417	a widely adopted RS material	390:417	a widely adopted RS material	390:417	Thus, the effect of raw potato starch (RPS), a widely adopted RS material, on microbial composition and barrier function of caecum for meat ducks was determined.
31495347	1	6	from	function	295:302	arg1	ducks					312:316	meat ducks	307:316	meat ducks	307:316	Resistant starch (RS) was recently approved to exert a powerful influence on gut health, but the effect of RS on the caecal barrier function in meat ducks has not been well defined.
31495347	3	7	theme	depth	727:731	arg1	ratio					733:737	crypt depth ratio	721:737	crypt depth ratio in the caecum	721:751	A total of 360 Cherry Valley male ducks of 1-d-old were randomly divided and fed diets with 0 (control), 12, or 24 % RPS for 35 d. Diets supplemented with RPS significantly elevated villus height and villus height:crypt depth ratio in the caecum.
31495347	3	8	dep	divided	572:578	arg1	ratio					733:737	crypt depth ratio	721:737	crypt depth ratio in the caecum	721:751	A total of 360 Cherry Valley male ducks of 1-d-old were randomly divided and fed diets with 0 (control), 12, or 24 % RPS for 35 d. Diets supplemented with RPS significantly elevated villus height and villus height:crypt depth ratio in the caecum.
31495347	2	9	theme	barrier	449:455	arg1	function					457:464	barrier function	449:464	barrier function of caecum for meat ducks	449:489	Thus, the effect of raw potato starch (RPS), a widely adopted RS material, on microbial composition and barrier function of caecum for meat ducks was determined.
31495347	8	10	theme	inflammatory	1702:1713	arg1	markers					1715:1721	inflammatory markers	1702:1721	inflammatory markers	1702:1721	These data revealed that diets supplemented with 12 % RPS partially improved caecal barrier function in meat ducks by enhancing intestinal morphology and barrier markers expression, modulating the microbiota composition and attenuating inflammatory markers.
31495347	8	11	theme	microbiota	1663:1672	arg1	composition					1674:1684	the microbiota composition	1659:1684	the microbiota composition	1659:1684	These data revealed that diets supplemented with 12 % RPS partially improved caecal barrier function in meat ducks by enhancing intestinal morphology and barrier markers expression, modulating the microbiota composition and attenuating inflammatory markers.
31495347	3	12	theme	elevated	680:687	arg1	RPS					662:664	RPS significantly elevated villus height and villus height	662:719	RPS significantly elevated villus height and villus height	662:719	A total of 360 Cherry Valley male ducks of 1-d-old were randomly divided and fed diets with 0 (control), 12, or 24 % RPS for 35 d. Diets supplemented with RPS significantly elevated villus height and villus height:crypt depth ratio in the caecum.
31495347	3	12	theme	elevated	680:687	arg1	height					696:701	significantly elevated villus height	666:701	significantly elevated villus height	666:701	A total of 360 Cherry Valley male ducks of 1-d-old were randomly divided and fed diets with 0 (control), 12, or 24 % RPS for 35 d. Diets supplemented with RPS significantly elevated villus height and villus height:crypt depth ratio in the caecum.
31495347	6	13	theme	acetate	1195:1201	arg1	contents					1228:1235	acetate, propionate and butyrate contents	1195:1235	acetate, propionate and butyrate contents	1195:1235	When compared with the control diet, the diet with 12 % RPS was also found to notably increase acetate, propionate and butyrate contents and up-regulated barrier-related genes including claudin-1, zonula occludens-1, mucin-2 and proglucagon in the caecum.
31495347	4	14	dep	bacteria	896:903	arg1	Faecalibacterium					905:920	Faecalibacterium	905:920	Faecalibacterium	905:920	The 16S rRNA sequence analysis indicated that the diet with 12 % RPS had a higher relative abundance of Firmicutes and the butyrate-producing bacteria Faecalibacterium, Subdoligranulum, and Erysipelatoclostridium were enriched in all diets.
31495347	4	14	dep	bacteria	896:903	arg1	bacteria					896:903	the butyrate-producing bacteria	873:903	the butyrate-producing bacteria Faecalibacterium, Subdoligranulum, and Erysipelatoclostridium	873:965	The 16S rRNA sequence analysis indicated that the diet with 12 % RPS had a higher relative abundance of Firmicutes and the butyrate-producing bacteria Faecalibacterium, Subdoligranulum, and Erysipelatoclostridium were enriched in all diets.
31495347	4	14	dep	bacteria	896:903	arg1	Subdoligranulum					923:937	Subdoligranulum	923:937	Subdoligranulum	923:937	The 16S rRNA sequence analysis indicated that the diet with 12 % RPS had a higher relative abundance of Firmicutes and the butyrate-producing bacteria Faecalibacterium, Subdoligranulum, and Erysipelatoclostridium were enriched in all diets.
31495347	4	14	dep	bacteria	896:903	arg1	Erysipelatoclostridium					944:965	Erysipelatoclostridium	944:965	Erysipelatoclostridium	944:965	The 16S rRNA sequence analysis indicated that the diet with 12 % RPS had a higher relative abundance of Firmicutes and the butyrate-producing bacteria Faecalibacterium, Subdoligranulum, and Erysipelatoclostridium were enriched in all diets.
31495347	3	15	from	ratio	733:737	arg1	caecum					746:751	the caecum	742:751	the caecum	742:751	A total of 360 Cherry Valley male ducks of 1-d-old were randomly divided and fed diets with 0 (control), 12, or 24 % RPS for 35 d. Diets supplemented with RPS significantly elevated villus height and villus height:crypt depth ratio in the caecum.
31495347	7	16	theme	plasma	1416:1421	arg1	TNF-α					1423:1427	plasma TNF-α	1416:1427	plasma TNF-α	1416:1427	Furthermore, the addition of 12 % RPS significantly reduced plasma TNF-α, IL-1β and endotoxin concentrations.
31495347	3	17	theme	villus	689:694	arg1	RPS					662:664	RPS significantly elevated villus height and villus height	662:719	RPS significantly elevated villus height and villus height	662:719	A total of 360 Cherry Valley male ducks of 1-d-old were randomly divided and fed diets with 0 (control), 12, or 24 % RPS for 35 d. Diets supplemented with RPS significantly elevated villus height and villus height:crypt depth ratio in the caecum.
31495347	3	17	theme	villus	689:694	arg1	height					696:701	significantly elevated villus height	666:701	significantly elevated villus height	666:701	A total of 360 Cherry Valley male ducks of 1-d-old were randomly divided and fed diets with 0 (control), 12, or 24 % RPS for 35 d. Diets supplemented with RPS significantly elevated villus height and villus height:crypt depth ratio in the caecum.
31495347	0	18	theme	microbiota	126:135	arg1	composition					137:147	microbiota composition	126:147	microbiota composition in meat duck	126:160	Dietary administration of resistant starch improved caecal barrier function by enhancing intestinal morphology and modulating microbiota composition in meat duck.
31495347	6	19	theme	control	1123:1129	arg1	diet					1131:1134	the control diet	1119:1134	the control diet	1119:1134	When compared with the control diet, the diet with 12 % RPS was also found to notably increase acetate, propionate and butyrate contents and up-regulated barrier-related genes including claudin-1, zonula occludens-1, mucin-2 and proglucagon in the caecum.
31495347	6	20	theme	barrier-related	1254:1268	arg1	mucin-2					1317:1323	mucin-2	1317:1323	mucin-2	1317:1323	When compared with the control diet, the diet with 12 % RPS was also found to notably increase acetate, propionate and butyrate contents and up-regulated barrier-related genes including claudin-1, zonula occludens-1, mucin-2 and proglucagon in the caecum.
31495347	6	20	theme	barrier-related	1254:1268	arg1	claudin-1					1286:1294	claudin-1	1286:1294	claudin-1	1286:1294	When compared with the control diet, the diet with 12 % RPS was also found to notably increase acetate, propionate and butyrate contents and up-regulated barrier-related genes including claudin-1, zonula occludens-1, mucin-2 and proglucagon in the caecum.
31495347	6	20	theme	barrier-related	1254:1268	arg1	occludens-1					1304:1314	zonula occludens-1	1297:1314	zonula occludens-1	1297:1314	When compared with the control diet, the diet with 12 % RPS was also found to notably increase acetate, propionate and butyrate contents and up-regulated barrier-related genes including claudin-1, zonula occludens-1, mucin-2 and proglucagon in the caecum.
31495347	6	20	theme	barrier-related	1254:1268	arg1	genes					1270:1274	barrier-related genes	1254:1274	barrier-related genes including claudin-1, zonula occludens-1, mucin-2 and proglucagon	1254:1339	When compared with the control diet, the diet with 12 % RPS was also found to notably increase acetate, propionate and butyrate contents and up-regulated barrier-related genes including claudin-1, zonula occludens-1, mucin-2 and proglucagon in the caecum.
31495347	6	20	theme	barrier-related	1254:1268	arg1	proglucagon					1329:1339	proglucagon	1329:1339	proglucagon	1329:1339	When compared with the control diet, the diet with 12 % RPS was also found to notably increase acetate, propionate and butyrate contents and up-regulated barrier-related genes including claudin-1, zonula occludens-1, mucin-2 and proglucagon in the caecum.
31495347	6	21	with	diet	1141:1144	arg1	RPS					1156:1158	12 % RPS	1151:1158	12 % RPS	1151:1158	When compared with the control diet, the diet with 12 % RPS was also found to notably increase acetate, propionate and butyrate contents and up-regulated barrier-related genes including claudin-1, zonula occludens-1, mucin-2 and proglucagon in the caecum.
31495347	4	22	theme	rRNA	762:765	arg1	analysis					776:783	The 16S rRNA sequence analysis	754:783	The 16S rRNA sequence analysis	754:783	The 16S rRNA sequence analysis indicated that the diet with 12 % RPS had a higher relative abundance of Firmicutes and the butyrate-producing bacteria Faecalibacterium, Subdoligranulum, and Erysipelatoclostridium were enriched in all diets.
31495347	2	23	theme	microbial	423:431	arg1	composition					433:443	microbial composition	423:443	microbial composition	423:443	Thus, the effect of raw potato starch (RPS), a widely adopted RS material, on microbial composition and barrier function of caecum for meat ducks was determined.
31495347	7	24	dep	TNF-α	1423:1427	arg1	concentrations					1450:1463	concentrations	1450:1463	concentrations	1450:1463	Furthermore, the addition of 12 % RPS significantly reduced plasma TNF-α, IL-1β and endotoxin concentrations.
31495347	4	25	theme	higher	829:834	arg1	abundance					845:853	a higher relative abundance	827:853	a higher relative abundance of Firmicutes	827:867	The 16S rRNA sequence analysis indicated that the diet with 12 % RPS had a higher relative abundance of Firmicutes and the butyrate-producing bacteria Faecalibacterium, Subdoligranulum, and Erysipelatoclostridium were enriched in all diets.
31495347	5	26	theme	24	1065:1066	arg1	%					1068:1068	%	1068:1068	%	1068:1068	Lactobacillus and Bifidobacterium were significantly increased in the 24 % RPS diet v. the control diet.
31495347	8	27	theme	meat	1570:1573	arg1	ducks					1575:1579	meat ducks	1570:1579	meat ducks	1570:1579	These data revealed that diets supplemented with 12 % RPS partially improved caecal barrier function in meat ducks by enhancing intestinal morphology and barrier markers expression, modulating the microbiota composition and attenuating inflammatory markers.
31495347	4	28	theme	Firmicutes	858:867	arg1	abundance					845:853	a higher relative abundance	827:853	a higher relative abundance of Firmicutes	827:867	The 16S rRNA sequence analysis indicated that the diet with 12 % RPS had a higher relative abundance of Firmicutes and the butyrate-producing bacteria Faecalibacterium, Subdoligranulum, and Erysipelatoclostridium were enriched in all diets.
31495347	6	29	theme	propionate	1204:1213	arg1	contents					1228:1235	acetate, propionate and butyrate contents	1195:1235	acetate, propionate and butyrate contents	1195:1235	When compared with the control diet, the diet with 12 % RPS was also found to notably increase acetate, propionate and butyrate contents and up-regulated barrier-related genes including claudin-1, zonula occludens-1, mucin-2 and proglucagon in the caecum.
31495347	1	30	from	effect	260:265	arg1	function					295:302	the caecal barrier function	276:302	the caecal barrier function in meat ducks	276:316	Resistant starch (RS) was recently approved to exert a powerful influence on gut health, but the effect of RS on the caecal barrier function in meat ducks has not been well defined.
31495347	8	31	theme	barrier	1550:1556	arg1	function					1558:1565	caecal barrier function	1543:1565	caecal barrier function	1543:1565	These data revealed that diets supplemented with 12 % RPS partially improved caecal barrier function in meat ducks by enhancing intestinal morphology and barrier markers expression, modulating the microbiota composition and attenuating inflammatory markers.
31495347	0	32	theme	Dietary	0:6	arg1	administration					8:21	Dietary administration	0:21	Dietary administration of resistant starch	0:41	Dietary administration of resistant starch improved caecal barrier function by enhancing intestinal morphology and modulating microbiota composition in meat duck.
31495347	7	33	theme	%	1388:1388	arg1	RPS					1390:1392	12 % RPS	1385:1392	12 % RPS	1385:1392	Furthermore, the addition of 12 % RPS significantly reduced plasma TNF-α, IL-1β and endotoxin concentrations.
31495347	0	34	theme	resistant	26:34	arg1	starch					36:41	resistant starch	26:41	resistant starch	26:41	Dietary administration of resistant starch improved caecal barrier function by enhancing intestinal morphology and modulating microbiota composition in meat duck.
31495347	4	35	theme	sequence	767:774	arg1	analysis					776:783	The 16S rRNA sequence analysis	754:783	The 16S rRNA sequence analysis	754:783	The 16S rRNA sequence analysis indicated that the diet with 12 % RPS had a higher relative abundance of Firmicutes and the butyrate-producing bacteria Faecalibacterium, Subdoligranulum, and Erysipelatoclostridium were enriched in all diets.
31495347	4	36	contain	had	823:825	arg1	diet					804:807	the diet	800:807	the diet with 12 % RPS	800:821	The 16S rRNA sequence analysis indicated that the diet with 12 % RPS had a higher relative abundance of Firmicutes and the butyrate-producing bacteria Faecalibacterium, Subdoligranulum, and Erysipelatoclostridium were enriched in all diets.
31495347	4	36	contain	had	823:825	arg2	abundance					845:853	a higher relative abundance	827:853	a higher relative abundance of Firmicutes	827:867	The 16S rRNA sequence analysis indicated that the diet with 12 % RPS had a higher relative abundance of Firmicutes and the butyrate-producing bacteria Faecalibacterium, Subdoligranulum, and Erysipelatoclostridium were enriched in all diets.
31495347	2	37	theme	potato	369:374	arg1	RPS					384:386	RPS	384:386	RPS	384:386	Thus, the effect of raw potato starch (RPS), a widely adopted RS material, on microbial composition and barrier function of caecum for meat ducks was determined.
31495347	2	37	theme	potato	369:374	arg1	starch					376:381	raw potato starch	365:381	raw potato starch (RPS)	365:387	Thus, the effect of raw potato starch (RPS), a widely adopted RS material, on microbial composition and barrier function of caecum for meat ducks was determined.
31495347	2	37	theme	potato	369:374	arg1	material					410:417	a widely adopted RS material	390:417	a widely adopted RS material	390:417	Thus, the effect of raw potato starch (RPS), a widely adopted RS material, on microbial composition and barrier function of caecum for meat ducks was determined.
31495347	2	38	theme	starch	376:381	arg1	effect					355:360	the effect	351:360	the effect of raw potato starch (RPS), a widely adopted RS material, on microbial composition and barrier function of caecum for meat ducks	351:489	Thus, the effect of raw potato starch (RPS), a widely adopted RS material, on microbial composition and barrier function of caecum for meat ducks was determined.
31495347	1	39	theme	RS	270:271	arg1	effect					260:265	the effect	256:265	the effect of RS on the caecal barrier function in meat ducks	256:316	Resistant starch (RS) was recently approved to exert a powerful influence on gut health, but the effect of RS on the caecal barrier function in meat ducks has not been well defined.
31495347	2	40	theme	caecum	469:474	arg1	composition					433:443	microbial composition	423:443	microbial composition	423:443	Thus, the effect of raw potato starch (RPS), a widely adopted RS material, on microbial composition and barrier function of caecum for meat ducks was determined.
31495347	2	40	theme	caecum	469:474	arg1	function					457:464	barrier function	449:464	barrier function of caecum for meat ducks	449:489	Thus, the effect of raw potato starch (RPS), a widely adopted RS material, on microbial composition and barrier function of caecum for meat ducks was determined.
31495347	3	41	theme	1-d-old	550:556	arg1	ducks					541:545	360 Cherry Valley male ducks	518:545	360 Cherry Valley male ducks of 1-d-old	518:556	A total of 360 Cherry Valley male ducks of 1-d-old were randomly divided and fed diets with 0 (control), 12, or 24 % RPS for 35 d. Diets supplemented with RPS significantly elevated villus height and villus height:crypt depth ratio in the caecum.
31495347	8	42	theme	intestinal	1594:1603	arg1	morphology					1605:1614	intestinal morphology	1594:1614	intestinal morphology	1594:1614	These data revealed that diets supplemented with 12 % RPS partially improved caecal barrier function in meat ducks by enhancing intestinal morphology and barrier markers expression, modulating the microbiota composition and attenuating inflammatory markers.
31495347	2	43	theme	raw	365:367	arg1	RPS					384:386	RPS	384:386	RPS	384:386	Thus, the effect of raw potato starch (RPS), a widely adopted RS material, on microbial composition and barrier function of caecum for meat ducks was determined.
31495347	2	43	theme	raw	365:367	arg1	starch					376:381	raw potato starch	365:381	raw potato starch (RPS)	365:387	Thus, the effect of raw potato starch (RPS), a widely adopted RS material, on microbial composition and barrier function of caecum for meat ducks was determined.
31495347	2	43	theme	raw	365:367	arg1	material					410:417	a widely adopted RS material	390:417	a widely adopted RS material	390:417	Thus, the effect of raw potato starch (RPS), a widely adopted RS material, on microbial composition and barrier function of caecum for meat ducks was determined.
31495347	2	44	theme	adopted	399:405	arg1	starch					376:381	raw potato starch	365:381	raw potato starch (RPS)	365:387	Thus, the effect of raw potato starch (RPS), a widely adopted RS material, on microbial composition and barrier function of caecum for meat ducks was determined.
31495347	2	44	theme	adopted	399:405	arg1	material					410:417	a widely adopted RS material	390:417	a widely adopted RS material	390:417	Thus, the effect of raw potato starch (RPS), a widely adopted RS material, on microbial composition and barrier function of caecum for meat ducks was determined.
31495347	5	45	theme	%	1068:1068	arg1	diet					1074:1077	the 24 % RPS diet	1061:1077	the 24 % RPS diet v. the control diet	1061:1097	Lactobacillus and Bifidobacterium were significantly increased in the 24 % RPS diet v. the control diet.
31495347	7	46	theme	12	1385:1386	arg1	%					1388:1388	%	1388:1388	%	1388:1388	Furthermore, the addition of 12 % RPS significantly reduced plasma TNF-α, IL-1β and endotoxin concentrations.
31495347	5	47	theme	control	1086:1092	arg1	diet					1094:1097	the control diet	1082:1097	the control diet	1082:1097	Lactobacillus and Bifidobacterium were significantly increased in the 24 % RPS diet v. the control diet.
31495347	6	48	theme	zonula	1297:1302	arg1	occludens-1					1304:1314	zonula occludens-1	1297:1314	zonula occludens-1	1297:1314	When compared with the control diet, the diet with 12 % RPS was also found to notably increase acetate, propionate and butyrate contents and up-regulated barrier-related genes including claudin-1, zonula occludens-1, mucin-2 and proglucagon in the caecum.
31495347	3	49	dep	0	599:599	arg1	RPS					624:626	RPS	624:626	RPS	624:626	A total of 360 Cherry Valley male ducks of 1-d-old were randomly divided and fed diets with 0 (control), 12, or 24 % RPS for 35 d. Diets supplemented with RPS significantly elevated villus height and villus height:crypt depth ratio in the caecum.
31495347	0	50	theme	starch	36:41	arg1	administration					8:21	Dietary administration	0:21	Dietary administration of resistant starch	0:41	Dietary administration of resistant starch improved caecal barrier function by enhancing intestinal morphology and modulating microbiota composition in meat duck.
31495347	3	51	theme	35 d.	632:636	arg1	Diets					638:642	35 d. Diets	632:642	35 d. Diets supplemented with RPS significantly elevated villus height and villus height	632:719	A total of 360 Cherry Valley male ducks of 1-d-old were randomly divided and fed diets with 0 (control), 12, or 24 % RPS for 35 d. Diets supplemented with RPS significantly elevated villus height and villus height:crypt depth ratio in the caecum.
31495347	0	52	theme	meat	152:155	arg1	duck					157:160	meat duck	152:160	meat duck	152:160	Dietary administration of resistant starch improved caecal barrier function by enhancing intestinal morphology and modulating microbiota composition in meat duck.
31495347	3	53	theme	ducks	541:545	arg1	total					509:513	A total	507:513	A total of 360 Cherry Valley male ducks of 1-d-old	507:556	A total of 360 Cherry Valley male ducks of 1-d-old were randomly divided and fed diets with 0 (control), 12, or 24 % RPS for 35 d. Diets supplemented with RPS significantly elevated villus height and villus height:crypt depth ratio in the caecum.
31495347	4	54	with	diet	804:807	arg1	RPS					819:821	12 % RPS	814:821	12 % RPS	814:821	The 16S rRNA sequence analysis indicated that the diet with 12 % RPS had a higher relative abundance of Firmicutes and the butyrate-producing bacteria Faecalibacterium, Subdoligranulum, and Erysipelatoclostridium were enriched in all diets.
31495347	0	55	theme	barrier	59:65	arg1	function					67:74	caecal barrier function	52:74	caecal barrier function	52:74	Dietary administration of resistant starch improved caecal barrier function by enhancing intestinal morphology and modulating microbiota composition in meat duck.
31495347	1	56	theme	caecal	280:285	arg1	function					295:302	the caecal barrier function	276:302	the caecal barrier function in meat ducks	276:316	Resistant starch (RS) was recently approved to exert a powerful influence on gut health, but the effect of RS on the caecal barrier function in meat ducks has not been well defined.
31495347	6	57	theme	butyrate	1219:1226	arg1	contents					1228:1235	acetate, propionate and butyrate contents	1195:1235	acetate, propionate and butyrate contents	1195:1235	When compared with the control diet, the diet with 12 % RPS was also found to notably increase acetate, propionate and butyrate contents and up-regulated barrier-related genes including claudin-1, zonula occludens-1, mucin-2 and proglucagon in the caecum.
31495347	6	58	theme	12	1151:1152	arg1	%					1154:1154	%	1154:1154	%	1154:1154	When compared with the control diet, the diet with 12 % RPS was also found to notably increase acetate, propionate and butyrate contents and up-regulated barrier-related genes including claudin-1, zonula occludens-1, mucin-2 and proglucagon in the caecum.
31495347	7	59	theme	RPS	1390:1392	arg1	addition					1373:1380	the addition	1369:1380	the addition of 12 % RPS	1369:1392	Furthermore, the addition of 12 % RPS significantly reduced plasma TNF-α, IL-1β and endotoxin concentrations.
31495347	0	60	theme	caecal	52:57	arg1	function					67:74	caecal barrier function	52:74	caecal barrier function	52:74	Dietary administration of resistant starch improved caecal barrier function by enhancing intestinal morphology and modulating microbiota composition in meat duck.
31495347	1	61	theme	barrier	287:293	arg1	function					295:302	the caecal barrier function	276:302	the caecal barrier function in meat ducks	276:316	Resistant starch (RS) was recently approved to exert a powerful influence on gut health, but the effect of RS on the caecal barrier function in meat ducks has not been well defined.
31495347	6	62	theme	%	1154:1154	arg1	RPS					1156:1158	12 % RPS	1151:1158	12 % RPS	1151:1158	When compared with the control diet, the diet with 12 % RPS was also found to notably increase acetate, propionate and butyrate contents and up-regulated barrier-related genes including claudin-1, zonula occludens-1, mucin-2 and proglucagon in the caecum.
31495347	8	63	theme	caecal	1543:1548	arg1	function					1558:1565	caecal barrier function	1543:1565	caecal barrier function	1543:1565	These data revealed that diets supplemented with 12 % RPS partially improved caecal barrier function in meat ducks by enhancing intestinal morphology and barrier markers expression, modulating the microbiota composition and attenuating inflammatory markers.
31495347	8	64	theme	markers	1628:1634	arg1	expression					1636:1645	barrier markers expression	1620:1645	barrier markers expression	1620:1645	These data revealed that diets supplemented with 12 % RPS partially improved caecal barrier function in meat ducks by enhancing intestinal morphology and barrier markers expression, modulating the microbiota composition and attenuating inflammatory markers.
31495347	3	65	dep	RPS	662:664	arg1	RPS					662:664	RPS significantly elevated villus height and villus height	662:719	RPS significantly elevated villus height and villus height	662:719	A total of 360 Cherry Valley male ducks of 1-d-old were randomly divided and fed diets with 0 (control), 12, or 24 % RPS for 35 d. Diets supplemented with RPS significantly elevated villus height and villus height:crypt depth ratio in the caecum.
31495347	3	65	dep	RPS	662:664	arg1	height					696:701	significantly elevated villus height	666:701	significantly elevated villus height	666:701	A total of 360 Cherry Valley male ducks of 1-d-old were randomly divided and fed diets with 0 (control), 12, or 24 % RPS for 35 d. Diets supplemented with RPS significantly elevated villus height and villus height:crypt depth ratio in the caecum.
31495347	3	65	dep	RPS	662:664	arg1	height					714:719	villus height	707:719	villus height	707:719	A total of 360 Cherry Valley male ducks of 1-d-old were randomly divided and fed diets with 0 (control), 12, or 24 % RPS for 35 d. Diets supplemented with RPS significantly elevated villus height and villus height:crypt depth ratio in the caecum.
31495347	4	66	theme	relative	836:843	arg1	abundance					845:853	a higher relative abundance	827:853	a higher relative abundance of Firmicutes	827:867	The 16S rRNA sequence analysis indicated that the diet with 12 % RPS had a higher relative abundance of Firmicutes and the butyrate-producing bacteria Faecalibacterium, Subdoligranulum, and Erysipelatoclostridium were enriched in all diets.
31495347	3	67	theme	Cherry	522:527	arg1	ducks					541:545	360 Cherry Valley male ducks	518:545	360 Cherry Valley male ducks of 1-d-old	518:556	A total of 360 Cherry Valley male ducks of 1-d-old were randomly divided and fed diets with 0 (control), 12, or 24 % RPS for 35 d. Diets supplemented with RPS significantly elevated villus height and villus height:crypt depth ratio in the caecum.
31495347	4	68	theme	16S	758:760	arg1	rRNA					762:765	The 16S rRNA	754:765	The 16S rRNA sequence analysis	754:783	The 16S rRNA sequence analysis indicated that the diet with 12 % RPS had a higher relative abundance of Firmicutes and the butyrate-producing bacteria Faecalibacterium, Subdoligranulum, and Erysipelatoclostridium were enriched in all diets.
31495347	3	69	theme	Valley	529:534	arg1	ducks					541:545	360 Cherry Valley male ducks	518:545	360 Cherry Valley male ducks of 1-d-old	518:556	A total of 360 Cherry Valley male ducks of 1-d-old were randomly divided and fed diets with 0 (control), 12, or 24 % RPS for 35 d. Diets supplemented with RPS significantly elevated villus height and villus height:crypt depth ratio in the caecum.
31495347	8	70	theme	barrier	1620:1626	arg1	expression					1636:1645	barrier markers expression	1620:1645	barrier markers expression	1620:1645	These data revealed that diets supplemented with 12 % RPS partially improved caecal barrier function in meat ducks by enhancing intestinal morphology and barrier markers expression, modulating the microbiota composition and attenuating inflammatory markers.
31495347	3	71	theme	crypt	721:725	arg1	depth					727:731	crypt depth	721:731	crypt depth ratio in the caecum	721:751	A total of 360 Cherry Valley male ducks of 1-d-old were randomly divided and fed diets with 0 (control), 12, or 24 % RPS for 35 d. Diets supplemented with RPS significantly elevated villus height and villus height:crypt depth ratio in the caecum.
31495347	1	72	theme	powerful	218:225	arg1	influence					227:235	a powerful influence	216:235	a powerful influence on gut health	216:249	Resistant starch (RS) was recently approved to exert a powerful influence on gut health, but the effect of RS on the caecal barrier function in meat ducks has not been well defined.
31495347	1	73	from	influence	227:235	arg1	health					244:249	gut health	240:249	gut health	240:249	Resistant starch (RS) was recently approved to exert a powerful influence on gut health, but the effect of RS on the caecal barrier function in meat ducks has not been well defined.
31495347	3	74	theme	male	536:539	arg1	ducks					541:545	360 Cherry Valley male ducks	518:545	360 Cherry Valley male ducks of 1-d-old	518:556	A total of 360 Cherry Valley male ducks of 1-d-old were randomly divided and fed diets with 0 (control), 12, or 24 % RPS for 35 d. Diets supplemented with RPS significantly elevated villus height and villus height:crypt depth ratio in the caecum.
31495347	3	75	theme	villus	707:712	arg1	RPS					662:664	RPS significantly elevated villus height and villus height	662:719	RPS significantly elevated villus height and villus height	662:719	A total of 360 Cherry Valley male ducks of 1-d-old were randomly divided and fed diets with 0 (control), 12, or 24 % RPS for 35 d. Diets supplemented with RPS significantly elevated villus height and villus height:crypt depth ratio in the caecum.
31495347	3	75	theme	villus	707:712	arg1	height					714:719	villus height	707:719	villus height	707:719	A total of 360 Cherry Valley male ducks of 1-d-old were randomly divided and fed diets with 0 (control), 12, or 24 % RPS for 35 d. Diets supplemented with RPS significantly elevated villus height and villus height:crypt depth ratio in the caecum.
31495347	4	76	theme	%	817:817	arg1	RPS					819:821	12 % RPS	814:821	12 % RPS	814:821	The 16S rRNA sequence analysis indicated that the diet with 12 % RPS had a higher relative abundance of Firmicutes and the butyrate-producing bacteria Faecalibacterium, Subdoligranulum, and Erysipelatoclostridium were enriched in all diets.
31495347	8	77	theme	%	1518:1518	arg1	RPS					1520:1522	12 % RPS	1515:1522	12 % RPS	1515:1522	These data revealed that diets supplemented with 12 % RPS partially improved caecal barrier function in meat ducks by enhancing intestinal morphology and barrier markers expression, modulating the microbiota composition and attenuating inflammatory markers.
31495347	2	78	from	effect	355:360	arg1	composition					433:443	microbial composition	423:443	microbial composition	423:443	Thus, the effect of raw potato starch (RPS), a widely adopted RS material, on microbial composition and barrier function of caecum for meat ducks was determined.
31495347	2	78	from	effect	355:360	arg1	function					457:464	barrier function	449:464	barrier function of caecum for meat ducks	449:489	Thus, the effect of raw potato starch (RPS), a widely adopted RS material, on microbial composition and barrier function of caecum for meat ducks was determined.
31495347	8	79	theme	12	1515:1516	arg1	%					1518:1518	%	1518:1518	%	1518:1518	These data revealed that diets supplemented with 12 % RPS partially improved caecal barrier function in meat ducks by enhancing intestinal morphology and barrier markers expression, modulating the microbiota composition and attenuating inflammatory markers.
31495347	4	80	theme	12	814:815	arg1	%					817:817	%	817:817	%	817:817	The 16S rRNA sequence analysis indicated that the diet with 12 % RPS had a higher relative abundance of Firmicutes and the butyrate-producing bacteria Faecalibacterium, Subdoligranulum, and Erysipelatoclostridium were enriched in all diets.
31495347	0	81	from	composition	137:147	arg1	duck					157:160	meat duck	152:160	meat duck	152:160	Dietary administration of resistant starch improved caecal barrier function by enhancing intestinal morphology and modulating microbiota composition in meat duck.
31495347	1	82	theme	Resistant	163:171	arg1	RS					181:182	RS	181:182	RS	181:182	Resistant starch (RS) was recently approved to exert a powerful influence on gut health, but the effect of RS on the caecal barrier function in meat ducks has not been well defined.
31495347	1	82	theme	Resistant	163:171	arg1	starch					173:178	Resistant starch	163:178	Resistant starch (RS)	163:183	Resistant starch (RS) was recently approved to exert a powerful influence on gut health, but the effect of RS on the caecal barrier function in meat ducks has not been well defined.
31495347	3	83	with	diets	588:592	arg1	0					599:599	0	599:599	0	599:599	A total of 360 Cherry Valley male ducks of 1-d-old were randomly divided and fed diets with 0 (control), 12, or 24 % RPS for 35 d. Diets supplemented with RPS significantly elevated villus height and villus height:crypt depth ratio in the caecum.
31495347	3	83	with	diets	588:592	arg1	%					622:622	24 %	619:622	24 %	619:622	A total of 360 Cherry Valley male ducks of 1-d-old were randomly divided and fed diets with 0 (control), 12, or 24 % RPS for 35 d. Diets supplemented with RPS significantly elevated villus height and villus height:crypt depth ratio in the caecum.
31495347	3	83	with	diets	588:592	arg1	control					602:608	control	602:608	control	602:608	A total of 360 Cherry Valley male ducks of 1-d-old were randomly divided and fed diets with 0 (control), 12, or 24 % RPS for 35 d. Diets supplemented with RPS significantly elevated villus height and villus height:crypt depth ratio in the caecum.
31495347	3	83	with	diets	588:592	arg1	12					612:613	12	612:613	12	612:613	A total of 360 Cherry Valley male ducks of 1-d-old were randomly divided and fed diets with 0 (control), 12, or 24 % RPS for 35 d. Diets supplemented with RPS significantly elevated villus height and villus height:crypt depth ratio in the caecum.
31495347	1	84	theme	gut	240:242	arg1	health					244:249	gut health	240:249	gut health	240:249	Resistant starch (RS) was recently approved to exert a powerful influence on gut health, but the effect of RS on the caecal barrier function in meat ducks has not been well defined.
29300997	8	0	theme	composition	1060:1070	arg1	studies					1138:1144	Monosaccharide composition, molecular weight, nuclear magnetic resonance, and immunolabeling studies	1045:1144	Monosaccharide composition, molecular weight, nuclear magnetic resonance, and immunolabeling studies	1045:1144	Monosaccharide composition, molecular weight, nuclear magnetic resonance, and immunolabeling studies demonstrated that both xylan biosynthesis (content) and fine structure were significantly affected in the uxt triple mutant, leading to phenotypes resembling those of the irx mutants.
29300997	7	1	theme	fiber	1032:1036	arg1	cells					1038:1042	interfascicular fiber cells	1016:1042	interfascicular fiber cells	1016:1042	Multiple uxt mutants were generated, including a triple mutant, which exhibited collapsed vessels and reduced cell wall thickness in interfascicular fiber cells.
29300997	11	2	theme	xylan	1771:1775	arg1	biosynthesis					1777:1788	xylan biosynthesis	1771:1788	xylan biosynthesis	1771:1788	Taken together, our results underpin the physiological roles of the UXT family in xylan biosynthesis and provide novel insights into the nucleotide sugar metabolism and trafficking in plants.
29300997	8	3	theme	molecular	1073:1081	arg1	weight					1083:1088	molecular weight	1073:1088	molecular weight	1073:1088	Monosaccharide composition, molecular weight, nuclear magnetic resonance, and immunolabeling studies demonstrated that both xylan biosynthesis (content) and fine structure were significantly affected in the uxt triple mutant, leading to phenotypes resembling those of the irx mutants.
29300997	7	4	theme	wall	998:1001	arg1	thickness					1003:1011	reduced cell wall thickness	985:1011	reduced cell wall thickness in interfascicular fiber cells	985:1042	Multiple uxt mutants were generated, including a triple mutant, which exhibited collapsed vessels and reduced cell wall thickness in interfascicular fiber cells.
29300997	10	5	theme	cytosolic	1649:1657	arg1	pool					1667:1670	the cytosolic UDP-Xyl pool	1645:1670	the cytosolic UDP-Xyl pool within the cell	1645:1686	Moreover, analysis of the nucleotide sugar composition of the uxt mutants indicated that nucleotide sugar interconversion is influenced by the cytosolic UDP-Xyl pool within the cell.
29300997	1	6	theme	UDP-glucuronic	174:187	arg1	synthases					230:238	also termed UDP-Xyl synthases	210:238	also termed UDP-Xyl synthases (UXSs)	210:245	UDP-xylose (UDP-Xyl) is synthesized by UDP-glucuronic acid decarboxylases, also termed UDP-Xyl synthases (UXSs).
29300997	1	6	theme	UDP-glucuronic	174:187	arg1	decarboxylases					194:207	UDP-glucuronic acid decarboxylases	174:207	UDP-glucuronic acid decarboxylases	174:207	UDP-xylose (UDP-Xyl) is synthesized by UDP-glucuronic acid decarboxylases, also termed UDP-Xyl synthases (UXSs).
29300997	8	7	theme	magnetic	1099:1106	arg1	resonance					1108:1116	nuclear magnetic resonance	1091:1116	nuclear magnetic resonance	1091:1116	Monosaccharide composition, molecular weight, nuclear magnetic resonance, and immunolabeling studies demonstrated that both xylan biosynthesis (content) and fine structure were significantly affected in the uxt triple mutant, leading to phenotypes resembling those of the irx mutants.
29300997	5	8	theme	single	579:584	arg1	mutants					586:592	single mutants	579:592	single mutants affected in the UDP-Xyl transporter 1 (UXT1)	579:637	However, while single mutants affected in the UDP-Xyl transporter 1 (UXT1) showed a substantial reduction in cell wall xylose content, a double mutant affected in UXT2 and UXT3 had no obvious effect on cell wall xylose deposition.
29300997	5	9	theme	xylose	776:781	arg1	deposition					783:792	cell wall xylose deposition	766:792	cell wall xylose deposition	766:792	However, while single mutants affected in the UDP-Xyl transporter 1 (UXT1) showed a substantial reduction in cell wall xylose content, a double mutant affected in UXT2 and UXT3 had no obvious effect on cell wall xylose deposition.
29300997	6	10	theme	UXT	871:873	arg1	family					875:880	the UXT family	867:880	the UXT family	867:880	This prompted us to further investigate redundancy among the members of the UXT family.
29300997	11	11	from	trafficking	1858:1868	arg1	plants					1873:1878	plants	1873:1878	plants	1873:1878	Taken together, our results underpin the physiological roles of the UXT family in xylan biosynthesis and provide novel insights into the nucleotide sugar metabolism and trafficking in plants.
29300997	2	12	theme	subcellular	333:343	arg1	location					345:352	their subcellular location	327:352	their subcellular location	327:352	The Arabidopsis genome encodes six UXSs, which fall into two groups based upon their subcellular location: the Golgi lumen and the cytosol.
29300997	11	13	theme	novel	1802:1806	arg1	insights					1808:1815	novel insights	1802:1815	novel insights into the nucleotide sugar metabolism and trafficking in plants	1802:1878	Taken together, our results underpin the physiological roles of the UXT family in xylan biosynthesis and provide novel insights into the nucleotide sugar metabolism and trafficking in plants.
29300997	8	14	theme	immunolabeling	1123:1136	arg1	studies					1138:1144	Monosaccharide composition, molecular weight, nuclear magnetic resonance, and immunolabeling studies	1045:1144	Monosaccharide composition, molecular weight, nuclear magnetic resonance, and immunolabeling studies	1045:1144	Monosaccharide composition, molecular weight, nuclear magnetic resonance, and immunolabeling studies demonstrated that both xylan biosynthesis (content) and fine structure were significantly affected in the uxt triple mutant, leading to phenotypes resembling those of the irx mutants.
29300997	2	15	theme	Golgi	359:363	arg1	UXSs					283:286	six UXSs	279:286	six UXSs	279:286	The Arabidopsis genome encodes six UXSs, which fall into two groups based upon their subcellular location: the Golgi lumen and the cytosol.
29300997	2	15	theme	Golgi	359:363	arg1	lumen					365:369	the Golgi lumen	355:369	the Golgi lumen	355:369	The Arabidopsis genome encodes six UXSs, which fall into two groups based upon their subcellular location: the Golgi lumen and the cytosol.
29300997	11	16	theme	physiological	1730:1742	arg1	roles					1744:1748	the physiological roles	1726:1748	the physiological roles of the UXT family in xylan biosynthesis	1726:1788	Taken together, our results underpin the physiological roles of the UXT family in xylan biosynthesis and provide novel insights into the nucleotide sugar metabolism and trafficking in plants.
29300997	1	17	theme	UDP-Xyl	222:228	arg1	UXSs					241:244	UXSs	241:244	UXSs	241:244	UDP-xylose (UDP-Xyl) is synthesized by UDP-glucuronic acid decarboxylases, also termed UDP-Xyl synthases (UXSs).
29300997	1	17	theme	UDP-Xyl	222:228	arg1	synthases					230:238	also termed UDP-Xyl synthases	210:238	also termed UDP-Xyl synthases (UXSs)	210:245	UDP-xylose (UDP-Xyl) is synthesized by UDP-glucuronic acid decarboxylases, also termed UDP-Xyl synthases (UXSs).
29300997	1	17	theme	UDP-Xyl	222:228	arg1	decarboxylases					194:207	UDP-glucuronic acid decarboxylases	174:207	UDP-glucuronic acid decarboxylases	174:207	UDP-xylose (UDP-Xyl) is synthesized by UDP-glucuronic acid decarboxylases, also termed UDP-Xyl synthases (UXSs).
29300997	0	18	from	lumen	113:117	arg1	Arabidopsis					122:132	Arabidopsis	122:132	Arabidopsis	122:132	Three UDP-xylose transporters participate in xylan biosynthesis by conveying cytosolic UDP-xylose into the Golgi lumen in Arabidopsis.
29300997	11	19	from	metabolism	1843:1852	arg1	plants					1873:1878	plants	1873:1878	plants	1873:1878	Taken together, our results underpin the physiological roles of the UXT family in xylan biosynthesis and provide novel insights into the nucleotide sugar metabolism and trafficking in plants.
29300997	7	20	theme	triple	932:937	arg1	mutant					939:944	a triple mutant	930:944	a triple mutant	930:944	Multiple uxt mutants were generated, including a triple mutant, which exhibited collapsed vessels and reduced cell wall thickness in interfascicular fiber cells.
29300997	2	21	theme	Arabidopsis	252:262	arg1	UXSs					283:286	six UXSs	279:286	six UXSs	279:286	The Arabidopsis genome encodes six UXSs, which fall into two groups based upon their subcellular location: the Golgi lumen and the cytosol.
29300997	2	21	theme	Arabidopsis	252:262	arg1	genome					264:269	The Arabidopsis genome	248:269	The Arabidopsis genome	248:269	The Arabidopsis genome encodes six UXSs, which fall into two groups based upon their subcellular location: the Golgi lumen and the cytosol.
29300997	7	22	theme	uxt	892:894	arg1	mutants					896:902	Multiple uxt mutants	883:902	Multiple uxt mutants	883:902	Multiple uxt mutants were generated, including a triple mutant, which exhibited collapsed vessels and reduced cell wall thickness in interfascicular fiber cells.
29300997	9	23	theme	filament	1408:1415	arg1	growth					1417:1422	reduced filament growth	1400:1422	reduced filament growth	1400:1422	Pollination was also impaired in the uxt triple mutant, likely due to reduced filament growth and anther dehiscence caused by alterations in the composition of the cell walls.
29300997	5	24	theme	xylose	683:688	arg1	content					690:696	cell wall xylose content	673:696	cell wall xylose content	673:696	However, while single mutants affected in the UDP-Xyl transporter 1 (UXT1) showed a substantial reduction in cell wall xylose content, a double mutant affected in UXT2 and UXT3 had no obvious effect on cell wall xylose deposition.
29300997	9	25	from	alterations	1456:1466	arg1	composition					1475:1485	the composition	1471:1485	the composition of the cell walls	1471:1503	Pollination was also impaired in the uxt triple mutant, likely due to reduced filament growth and anther dehiscence caused by alterations in the composition of the cell walls.
29300997	8	26	theme	uxt	1252:1254	arg1	mutant					1263:1268	the uxt triple mutant	1248:1268	the uxt triple mutant	1248:1268	Monosaccharide composition, molecular weight, nuclear magnetic resonance, and immunolabeling studies demonstrated that both xylan biosynthesis (content) and fine structure were significantly affected in the uxt triple mutant, leading to phenotypes resembling those of the irx mutants.
29300997	5	27	theme	cell	673:676	arg1	content					690:696	cell wall xylose content	673:696	cell wall xylose content	673:696	However, while single mutants affected in the UDP-Xyl transporter 1 (UXT1) showed a substantial reduction in cell wall xylose content, a double mutant affected in UXT2 and UXT3 had no obvious effect on cell wall xylose deposition.
29300997	0	28	theme	cytosolic	77:85	arg1	UDP-xylose					87:96	cytosolic UDP-xylose	77:96	cytosolic UDP-xylose	77:96	Three UDP-xylose transporters participate in xylan biosynthesis by conveying cytosolic UDP-xylose into the Golgi lumen in Arabidopsis.
29300997	10	29	theme	sugar	1543:1547	arg1	composition					1549:1559	the nucleotide sugar composition	1528:1559	the nucleotide sugar composition of the uxt mutants	1528:1578	Moreover, analysis of the nucleotide sugar composition of the uxt mutants indicated that nucleotide sugar interconversion is influenced by the cytosolic UDP-Xyl pool within the cell.
29300997	5	30	contain	had	741:743	arg1	mutant					708:713	a double mutant	699:713	a double mutant affected in UXT2 and UXT3	699:739	However, while single mutants affected in the UDP-Xyl transporter 1 (UXT1) showed a substantial reduction in cell wall xylose content, a double mutant affected in UXT2 and UXT3 had no obvious effect on cell wall xylose deposition.
29300997	5	30	contain	had	741:743	arg2	effect					756:761	no obvious effect	745:761	no obvious effect	745:761	However, while single mutants affected in the UDP-Xyl transporter 1 (UXT1) showed a substantial reduction in cell wall xylose content, a double mutant affected in UXT2 and UXT3 had no obvious effect on cell wall xylose deposition.
29300997	11	31	theme	sugar	1837:1841	arg1	metabolism					1843:1852	nucleotide sugar metabolism	1826:1852	nucleotide sugar metabolism	1826:1852	Taken together, our results underpin the physiological roles of the UXT family in xylan biosynthesis and provide novel insights into the nucleotide sugar metabolism and trafficking in plants.
29300997	10	32	theme	uxt	1568:1570	arg1	mutants					1572:1578	the uxt mutants	1564:1578	the uxt mutants	1564:1578	Moreover, analysis of the nucleotide sugar composition of the uxt mutants indicated that nucleotide sugar interconversion is influenced by the cytosolic UDP-Xyl pool within the cell.
29300997	5	33	theme	double	701:706	arg1	mutant					708:713	a double mutant	699:713	a double mutant affected in UXT2 and UXT3	699:739	However, while single mutants affected in the UDP-Xyl transporter 1 (UXT1) showed a substantial reduction in cell wall xylose content, a double mutant affected in UXT2 and UXT3 had no obvious effect on cell wall xylose deposition.
29300997	9	34	theme	walls	1499:1503	arg1	composition					1475:1485	the composition	1471:1485	the composition of the cell walls	1471:1503	Pollination was also impaired in the uxt triple mutant, likely due to reduced filament growth and anther dehiscence caused by alterations in the composition of the cell walls.
29300997	4	35	theme	Golgi	504:508	arg1	lumen					510:514	the Golgi lumen	500:514	the Golgi lumen	500:514	Cytosolic UDP-Xyl is transported into the Golgi lumen by three UDP-Xyl transporters (UXT1, 2, and 3).
29300997	11	36	theme	UXT	1757:1759	arg1	family					1761:1766	the UXT family	1753:1766	the UXT family	1753:1766	Taken together, our results underpin the physiological roles of the UXT family in xylan biosynthesis and provide novel insights into the nucleotide sugar metabolism and trafficking in plants.
29300997	10	37	theme	nucleotide	1595:1604	arg1	interconversion					1612:1626	nucleotide sugar interconversion	1595:1626	nucleotide sugar interconversion	1595:1626	Moreover, analysis of the nucleotide sugar composition of the uxt mutants indicated that nucleotide sugar interconversion is influenced by the cytosolic UDP-Xyl pool within the cell.
29300997	3	38	theme	important	424:432	arg1	role					434:437	an important role	421:437	an important role	421:437	The latter group appears to play an important role in xylan biosynthesis.
29300997	5	39	theme	cell	766:769	arg1	deposition					783:792	cell wall xylose deposition	766:792	cell wall xylose deposition	766:792	However, while single mutants affected in the UDP-Xyl transporter 1 (UXT1) showed a substantial reduction in cell wall xylose content, a double mutant affected in UXT2 and UXT3 had no obvious effect on cell wall xylose deposition.
29300997	11	40	dep	metabolism	1843:1852	arg1	the					1822:1824	the	1822:1824	the	1822:1824	Taken together, our results underpin the physiological roles of the UXT family in xylan biosynthesis and provide novel insights into the nucleotide sugar metabolism and trafficking in plants.
29300997	7	41	theme	interfascicular	1016:1030	arg1	cells					1038:1042	interfascicular fiber cells	1016:1042	interfascicular fiber cells	1016:1042	Multiple uxt mutants were generated, including a triple mutant, which exhibited collapsed vessels and reduced cell wall thickness in interfascicular fiber cells.
29300997	8	42	theme	Monosaccharide	1045:1058	arg1	composition					1060:1070	Monosaccharide composition	1045:1070	Monosaccharide composition	1045:1070	Monosaccharide composition, molecular weight, nuclear magnetic resonance, and immunolabeling studies demonstrated that both xylan biosynthesis (content) and fine structure were significantly affected in the uxt triple mutant, leading to phenotypes resembling those of the irx mutants.
29300997	11	43	from	roles	1744:1748	arg1	biosynthesis					1777:1788	xylan biosynthesis	1771:1788	xylan biosynthesis	1771:1788	Taken together, our results underpin the physiological roles of the UXT family in xylan biosynthesis and provide novel insights into the nucleotide sugar metabolism and trafficking in plants.
29300997	7	44	from	vessels	973:979	arg1	cells					1038:1042	interfascicular fiber cells	1016:1042	interfascicular fiber cells	1016:1042	Multiple uxt mutants were generated, including a triple mutant, which exhibited collapsed vessels and reduced cell wall thickness in interfascicular fiber cells.
29300997	7	45	theme	cell	993:996	arg1	thickness					1003:1011	reduced cell wall thickness	985:1011	reduced cell wall thickness in interfascicular fiber cells	985:1042	Multiple uxt mutants were generated, including a triple mutant, which exhibited collapsed vessels and reduced cell wall thickness in interfascicular fiber cells.
29300997	10	46	theme	UDP-Xyl	1659:1665	arg1	pool					1667:1670	the cytosolic UDP-Xyl pool	1645:1670	the cytosolic UDP-Xyl pool within the cell	1645:1686	Moreover, analysis of the nucleotide sugar composition of the uxt mutants indicated that nucleotide sugar interconversion is influenced by the cytosolic UDP-Xyl pool within the cell.
29300997	1	47	theme	acid	189:192	arg1	synthases					230:238	also termed UDP-Xyl synthases	210:238	also termed UDP-Xyl synthases (UXSs)	210:245	UDP-xylose (UDP-Xyl) is synthesized by UDP-glucuronic acid decarboxylases, also termed UDP-Xyl synthases (UXSs).
29300997	1	47	theme	acid	189:192	arg1	decarboxylases					194:207	UDP-glucuronic acid decarboxylases	174:207	UDP-glucuronic acid decarboxylases	174:207	UDP-xylose (UDP-Xyl) is synthesized by UDP-glucuronic acid decarboxylases, also termed UDP-Xyl synthases (UXSs).
29300997	8	48	theme	weight	1083:1088	arg1	studies					1138:1144	Monosaccharide composition, molecular weight, nuclear magnetic resonance, and immunolabeling studies	1045:1144	Monosaccharide composition, molecular weight, nuclear magnetic resonance, and immunolabeling studies	1045:1144	Monosaccharide composition, molecular weight, nuclear magnetic resonance, and immunolabeling studies demonstrated that both xylan biosynthesis (content) and fine structure were significantly affected in the uxt triple mutant, leading to phenotypes resembling those of the irx mutants.
29300997	5	49	theme	wall	771:774	arg1	deposition					783:792	cell wall xylose deposition	766:792	cell wall xylose deposition	766:792	However, while single mutants affected in the UDP-Xyl transporter 1 (UXT1) showed a substantial reduction in cell wall xylose content, a double mutant affected in UXT2 and UXT3 had no obvious effect on cell wall xylose deposition.
29300997	8	50	theme	nuclear	1091:1097	arg1	resonance					1108:1116	nuclear magnetic resonance	1091:1116	nuclear magnetic resonance	1091:1116	Monosaccharide composition, molecular weight, nuclear magnetic resonance, and immunolabeling studies demonstrated that both xylan biosynthesis (content) and fine structure were significantly affected in the uxt triple mutant, leading to phenotypes resembling those of the irx mutants.
29300997	6	51	theme	family	875:880	arg1	members					856:862	the members	852:862	the members of the UXT family	852:880	This prompted us to further investigate redundancy among the members of the UXT family.
29300997	7	52	theme	reduced	985:991	arg1	thickness					1003:1011	reduced cell wall thickness	985:1011	reduced cell wall thickness in interfascicular fiber cells	985:1042	Multiple uxt mutants were generated, including a triple mutant, which exhibited collapsed vessels and reduced cell wall thickness in interfascicular fiber cells.
29300997	8	53	theme	xylan	1169:1173	arg1	content					1189:1195	content	1189:1195	content	1189:1195	Monosaccharide composition, molecular weight, nuclear magnetic resonance, and immunolabeling studies demonstrated that both xylan biosynthesis (content) and fine structure were significantly affected in the uxt triple mutant, leading to phenotypes resembling those of the irx mutants.
29300997	8	53	theme	xylan	1169:1173	arg1	biosynthesis					1175:1186	xylan biosynthesis	1169:1186	xylan biosynthesis (content)	1169:1196	Monosaccharide composition, molecular weight, nuclear magnetic resonance, and immunolabeling studies demonstrated that both xylan biosynthesis (content) and fine structure were significantly affected in the uxt triple mutant, leading to phenotypes resembling those of the irx mutants.
29300997	7	54	theme	collapsed	963:971	arg1	vessels					973:979	collapsed vessels	963:979	collapsed vessels	963:979	Multiple uxt mutants were generated, including a triple mutant, which exhibited collapsed vessels and reduced cell wall thickness in interfascicular fiber cells.
29300997	9	55	theme	triple	1371:1376	arg1	mutant					1378:1383	the uxt triple mutant	1363:1383	the uxt triple mutant	1363:1383	Pollination was also impaired in the uxt triple mutant, likely due to reduced filament growth and anther dehiscence caused by alterations in the composition of the cell walls.
29300997	8	56	theme	resonance	1108:1116	arg1	studies					1138:1144	Monosaccharide composition, molecular weight, nuclear magnetic resonance, and immunolabeling studies	1045:1144	Monosaccharide composition, molecular weight, nuclear magnetic resonance, and immunolabeling studies	1045:1144	Monosaccharide composition, molecular weight, nuclear magnetic resonance, and immunolabeling studies demonstrated that both xylan biosynthesis (content) and fine structure were significantly affected in the uxt triple mutant, leading to phenotypes resembling those of the irx mutants.
29300997	11	57	theme	family	1761:1766	arg1	roles					1744:1748	the physiological roles	1726:1748	the physiological roles of the UXT family in xylan biosynthesis	1726:1788	Taken together, our results underpin the physiological roles of the UXT family in xylan biosynthesis and provide novel insights into the nucleotide sugar metabolism and trafficking in plants.
29300997	9	58	theme	uxt	1367:1369	arg1	mutant					1378:1383	the uxt triple mutant	1363:1383	the uxt triple mutant	1363:1383	Pollination was also impaired in the uxt triple mutant, likely due to reduced filament growth and anther dehiscence caused by alterations in the composition of the cell walls.
29300997	5	59	theme	obvious	748:754	arg1	effect					756:761	no obvious effect	745:761	no obvious effect	745:761	However, while single mutants affected in the UDP-Xyl transporter 1 (UXT1) showed a substantial reduction in cell wall xylose content, a double mutant affected in UXT2 and UXT3 had no obvious effect on cell wall xylose deposition.
29300997	5	60	theme	substantial	648:658	arg1	reduction					660:668	a substantial reduction	646:668	a substantial reduction in cell wall xylose content	646:696	However, while single mutants affected in the UDP-Xyl transporter 1 (UXT1) showed a substantial reduction in cell wall xylose content, a double mutant affected in UXT2 and UXT3 had no obvious effect on cell wall xylose deposition.
29300997	5	61	theme	UDP-Xyl	610:616	arg1	transporter					618:628	UDP-Xyl transporter 1	610:630	the UDP-Xyl transporter 1 (UXT1)	606:637	However, while single mutants affected in the UDP-Xyl transporter 1 (UXT1) showed a substantial reduction in cell wall xylose content, a double mutant affected in UXT2 and UXT3 had no obvious effect on cell wall xylose deposition.
29300997	5	61	theme	UDP-Xyl	610:616	arg1	UXT1					633:636	UXT1	633:636	UXT1	633:636	However, while single mutants affected in the UDP-Xyl transporter 1 (UXT1) showed a substantial reduction in cell wall xylose content, a double mutant affected in UXT2 and UXT3 had no obvious effect on cell wall xylose deposition.
29300997	5	62	from	reduction	660:668	arg1	content					690:696	cell wall xylose content	673:696	cell wall xylose content	673:696	However, while single mutants affected in the UDP-Xyl transporter 1 (UXT1) showed a substantial reduction in cell wall xylose content, a double mutant affected in UXT2 and UXT3 had no obvious effect on cell wall xylose deposition.
29300997	9	63	theme	due	1393:1395	arg1	mutant					1378:1383	the uxt triple mutant	1363:1383	the uxt triple mutant	1363:1383	Pollination was also impaired in the uxt triple mutant, likely due to reduced filament growth and anther dehiscence caused by alterations in the composition of the cell walls.
29300997	8	64	theme	fine	1202:1205	arg1	structure					1207:1215	fine structure	1202:1215	fine structure	1202:1215	Monosaccharide composition, molecular weight, nuclear magnetic resonance, and immunolabeling studies demonstrated that both xylan biosynthesis (content) and fine structure were significantly affected in the uxt triple mutant, leading to phenotypes resembling those of the irx mutants.
29300997	9	65	theme	reduced	1400:1406	arg1	growth					1417:1422	reduced filament growth	1400:1422	reduced filament growth	1400:1422	Pollination was also impaired in the uxt triple mutant, likely due to reduced filament growth and anther dehiscence caused by alterations in the composition of the cell walls.
29300997	3	66	theme	xylan	442:446	arg1	biosynthesis					448:459	xylan biosynthesis	442:459	xylan biosynthesis	442:459	The latter group appears to play an important role in xylan biosynthesis.
29300997	5	67	theme	wall	678:681	arg1	content					690:696	cell wall xylose content	673:696	cell wall xylose content	673:696	However, while single mutants affected in the UDP-Xyl transporter 1 (UXT1) showed a substantial reduction in cell wall xylose content, a double mutant affected in UXT2 and UXT3 had no obvious effect on cell wall xylose deposition.
29300997	7	68	theme	Multiple	883:890	arg1	mutants					896:902	Multiple uxt mutants	883:902	Multiple uxt mutants	883:902	Multiple uxt mutants were generated, including a triple mutant, which exhibited collapsed vessels and reduced cell wall thickness in interfascicular fiber cells.
29300997	9	69	theme	anther	1428:1433	arg1	dehiscence					1435:1444	anther dehiscence	1428:1444	anther dehiscence	1428:1444	Pollination was also impaired in the uxt triple mutant, likely due to reduced filament growth and anther dehiscence caused by alterations in the composition of the cell walls.
29300997	2	70	dep	UXSs	283:286	arg1	UXSs					283:286	six UXSs	279:286	six UXSs	279:286	The Arabidopsis genome encodes six UXSs, which fall into two groups based upon their subcellular location: the Golgi lumen and the cytosol.
29300997	2	70	dep	UXSs	283:286	arg1	lumen					365:369	the Golgi lumen	355:369	the Golgi lumen	355:369	The Arabidopsis genome encodes six UXSs, which fall into two groups based upon their subcellular location: the Golgi lumen and the cytosol.
29300997	2	70	dep	UXSs	283:286	arg1	cytosol					379:385	the cytosol	375:385	the cytosol	375:385	The Arabidopsis genome encodes six UXSs, which fall into two groups based upon their subcellular location: the Golgi lumen and the cytosol.
29300997	0	71	theme	Golgi	107:111	arg1	lumen					113:117	the Golgi lumen	103:117	the Golgi lumen in Arabidopsis	103:132	Three UDP-xylose transporters participate in xylan biosynthesis by conveying cytosolic UDP-xylose into the Golgi lumen in Arabidopsis.
29300997	8	72	theme	triple	1256:1261	arg1	mutant					1263:1268	the uxt triple mutant	1248:1268	the uxt triple mutant	1248:1268	Monosaccharide composition, molecular weight, nuclear magnetic resonance, and immunolabeling studies demonstrated that both xylan biosynthesis (content) and fine structure were significantly affected in the uxt triple mutant, leading to phenotypes resembling those of the irx mutants.
29300997	4	73	dep	transporters	533:544	arg1	2					553:553	2	553:553	2	553:553	Cytosolic UDP-Xyl is transported into the Golgi lumen by three UDP-Xyl transporters (UXT1, 2, and 3).
29300997	4	73	dep	transporters	533:544	arg1	3					560:560	3	560:560	3	560:560	Cytosolic UDP-Xyl is transported into the Golgi lumen by three UDP-Xyl transporters (UXT1, 2, and 3).
29300997	4	73	dep	transporters	533:544	arg1	UXT1					547:550	UXT1	547:550	UXT1	547:550	Cytosolic UDP-Xyl is transported into the Golgi lumen by three UDP-Xyl transporters (UXT1, 2, and 3).
29300997	4	74	theme	Cytosolic	462:470	arg1	UDP-Xyl					472:478	Cytosolic UDP-Xyl	462:478	Cytosolic UDP-Xyl	462:478	Cytosolic UDP-Xyl is transported into the Golgi lumen by three UDP-Xyl transporters (UXT1, 2, and 3).
29300997	10	75	theme	composition	1549:1559	arg1	analysis					1516:1523	analysis	1516:1523	analysis of the nucleotide sugar composition of the uxt mutants	1516:1578	Moreover, analysis of the nucleotide sugar composition of the uxt mutants indicated that nucleotide sugar interconversion is influenced by the cytosolic UDP-Xyl pool within the cell.
29300997	0	76	theme	UDP-xylose	6:15	arg1	transporters					17:28	Three UDP-xylose transporters	0:28	Three UDP-xylose transporters	0:28	Three UDP-xylose transporters participate in xylan biosynthesis by conveying cytosolic UDP-xylose into the Golgi lumen in Arabidopsis.
29300997	4	77	theme	UDP-Xyl	525:531	arg1	transporters					533:544	three UDP-Xyl transporters	519:544	three UDP-Xyl transporters (UXT1, 2, and 3)	519:561	Cytosolic UDP-Xyl is transported into the Golgi lumen by three UDP-Xyl transporters (UXT1, 2, and 3).
29300997	10	78	theme	nucleotide	1532:1541	arg1	composition					1549:1559	the nucleotide sugar composition	1528:1559	the nucleotide sugar composition of the uxt mutants	1528:1578	Moreover, analysis of the nucleotide sugar composition of the uxt mutants indicated that nucleotide sugar interconversion is influenced by the cytosolic UDP-Xyl pool within the cell.
29300997	10	79	theme	mutants	1572:1578	arg1	composition					1549:1559	the nucleotide sugar composition	1528:1559	the nucleotide sugar composition of the uxt mutants	1528:1578	Moreover, analysis of the nucleotide sugar composition of the uxt mutants indicated that nucleotide sugar interconversion is influenced by the cytosolic UDP-Xyl pool within the cell.
29300997	0	80	theme	xylan	45:49	arg1	biosynthesis					51:62	xylan biosynthesis	45:62	xylan biosynthesis	45:62	Three UDP-xylose transporters participate in xylan biosynthesis by conveying cytosolic UDP-xylose into the Golgi lumen in Arabidopsis.
29300997	1	81	theme	termed	215:220	arg1	UXSs					241:244	UXSs	241:244	UXSs	241:244	UDP-xylose (UDP-Xyl) is synthesized by UDP-glucuronic acid decarboxylases, also termed UDP-Xyl synthases (UXSs).
29300997	1	81	theme	termed	215:220	arg1	synthases					230:238	also termed UDP-Xyl synthases	210:238	also termed UDP-Xyl synthases (UXSs)	210:245	UDP-xylose (UDP-Xyl) is synthesized by UDP-glucuronic acid decarboxylases, also termed UDP-Xyl synthases (UXSs).
29300997	1	81	theme	termed	215:220	arg1	decarboxylases					194:207	UDP-glucuronic acid decarboxylases	174:207	UDP-glucuronic acid decarboxylases	174:207	UDP-xylose (UDP-Xyl) is synthesized by UDP-glucuronic acid decarboxylases, also termed UDP-Xyl synthases (UXSs).
29300997	9	82	theme	cell	1494:1497	arg1	walls					1499:1503	the cell walls	1490:1503	the cell walls	1490:1503	Pollination was also impaired in the uxt triple mutant, likely due to reduced filament growth and anther dehiscence caused by alterations in the composition of the cell walls.
29300997	3	83	theme	latter	392:397	arg1	group					399:403	The latter group	388:403	The latter group	388:403	The latter group appears to play an important role in xylan biosynthesis.
29300997	7	84	from	thickness	1003:1011	arg1	cells					1038:1042	interfascicular fiber cells	1016:1042	interfascicular fiber cells	1016:1042	Multiple uxt mutants were generated, including a triple mutant, which exhibited collapsed vessels and reduced cell wall thickness in interfascicular fiber cells.
29300997	11	85	theme	nucleotide	1826:1835	arg1	metabolism					1843:1852	nucleotide sugar metabolism	1826:1852	nucleotide sugar metabolism	1826:1852	Taken together, our results underpin the physiological roles of the UXT family in xylan biosynthesis and provide novel insights into the nucleotide sugar metabolism and trafficking in plants.
29300997	8	86	theme	irx	1317:1319	arg1	mutants					1321:1327	the irx mutants	1313:1327	the irx mutants	1313:1327	Monosaccharide composition, molecular weight, nuclear magnetic resonance, and immunolabeling studies demonstrated that both xylan biosynthesis (content) and fine structure were significantly affected in the uxt triple mutant, leading to phenotypes resembling those of the irx mutants.
29300997	10	87	theme	sugar	1606:1610	arg1	interconversion					1612:1626	nucleotide sugar interconversion	1595:1626	nucleotide sugar interconversion	1595:1626	Moreover, analysis of the nucleotide sugar composition of the uxt mutants indicated that nucleotide sugar interconversion is influenced by the cytosolic UDP-Xyl pool within the cell.
31155546	8	0	contain	have	1037:1040	arg2	correlation					1049:1059	a high correlation	1042:1059	a high correlation with the standard spectrophotometric assay (0.92 - 0.95%), which implies a possible application of the fabricated biosensors in foodstuff analysis	1042:1206	The biosensors' data on the glucose contents were shown to have a high correlation with the standard spectrophotometric assay (0.92 - 0.95%), which implies a possible application of the fabricated biosensors in foodstuff analysis.
31155546	8	0	contain	have	1037:1040	arg1	data					994:997	The biosensors' data	978:997	The biosensors' data on the glucose contents	978:1021	The biosensors' data on the glucose contents were shown to have a high correlation with the standard spectrophotometric assay (0.92 - 0.95%), which implies a possible application of the fabricated biosensors in foodstuff analysis.
31155546	1	1	theme	biosensor	173:181	arg1	characteristics					135:149	The characteristics	131:149	The characteristics of an electrochemical biosensor based on a Prussian-blue screen-printed electrode containing glucose oxidase incorporated into polyelectrolyte microcapsules (PMC)	131:312	The characteristics of an electrochemical biosensor based on a Prussian-blue screen-printed electrode containing glucose oxidase incorporated into polyelectrolyte microcapsules (PMC) are considered.
31155546	8	2	theme	biosensors	1175:1184	arg1	application					1145:1155	a possible application	1134:1155	a possible application of the fabricated biosensors	1134:1184	The biosensors' data on the glucose contents were shown to have a high correlation with the standard spectrophotometric assay (0.92 - 0.95%), which implies a possible application of the fabricated biosensors in foodstuff analysis.
31155546	4	3	theme	biosensor	561:569	arg1	signals					571:577	biosensor signals	561:577	biosensor signals	561:577	We assessed the dependences of biosensor signals on the composition of the buffer solution, on the glucose concentration; the operational and long-term stabilities.
31155546	4	4	theme	glucose	629:635	arg1	concentration					637:649	the glucose concentration	625:649	the glucose concentration	625:649	We assessed the dependences of biosensor signals on the composition of the buffer solution, on the glucose concentration; the operational and long-term stabilities.
31155546	8	5	from	data	994:997	arg1	contents					1014:1021	the glucose contents	1002:1021	the glucose contents	1002:1021	The biosensors' data on the glucose contents were shown to have a high correlation with the standard spectrophotometric assay (0.92 - 0.95%), which implies a possible application of the fabricated biosensors in foodstuff analysis.
31155546	8	6	theme	high	1044:1047	arg1	correlation					1049:1059	a high correlation	1042:1059	a high correlation with the standard spectrophotometric assay (0.92 - 0.95%), which implies a possible application of the fabricated biosensors in foodstuff analysis	1042:1206	The biosensors' data on the glucose contents were shown to have a high correlation with the standard spectrophotometric assay (0.92 - 0.95%), which implies a possible application of the fabricated biosensors in foodstuff analysis.
31155546	6	7	theme	Michaelis	817:825	arg1	mM					854:855	1.5 and 4.1 mM	842:855	1.5 and 4.1 mM	842:855	The apparent Michaelis constants were 1.5 and 4.1 mM at the immobilization in, respectively, chitosan and PMC.
31155546	6	7	theme	Michaelis	817:825	arg1	constants					827:835	The apparent Michaelis constants	804:835	The apparent Michaelis constants	804:835	The apparent Michaelis constants were 1.5 and 4.1 mM at the immobilization in, respectively, chitosan and PMC.
31155546	0	8	theme	Chitosan	117:124	arg1	Gel					126:128	Chitosan Gel	117:128	Chitosan Gel	117:128	Comparative Study of Electrochemical Sensors Based on Enzyme Immobilized into Polyelectrolyte Microcapsules and into Chitosan Gel.
31155546	8	9	theme	foodstuff	1189:1197	arg1	analysis					1199:1206	foodstuff analysis	1189:1206	foodstuff analysis	1189:1206	The biosensors' data on the glucose contents were shown to have a high correlation with the standard spectrophotometric assay (0.92 - 0.95%), which implies a possible application of the fabricated biosensors in foodstuff analysis.
31155546	6	10	theme	apparent	808:815	arg1	mM					854:855	1.5 and 4.1 mM	842:855	1.5 and 4.1 mM	842:855	The apparent Michaelis constants were 1.5 and 4.1 mM at the immobilization in, respectively, chitosan and PMC.
31155546	6	10	theme	apparent	808:815	arg1	constants					827:835	The apparent Michaelis constants	804:835	The apparent Michaelis constants	804:835	The apparent Michaelis constants were 1.5 and 4.1 mM at the immobilization in, respectively, chitosan and PMC.
31155546	6	11	from	immobilization	864:877	arg1	mM					854:855	1.5 and 4.1 mM	842:855	1.5 and 4.1 mM	842:855	The apparent Michaelis constants were 1.5 and 4.1 mM at the immobilization in, respectively, chitosan and PMC.
31155546	6	11	from	immobilization	864:877	arg1	constants					827:835	The apparent Michaelis constants	804:835	The apparent Michaelis constants	804:835	The apparent Michaelis constants were 1.5 and 4.1 mM at the immobilization in, respectively, chitosan and PMC.
31155546	1	12	theme	Prussian-blue	194:206	arg1	electrode					223:231	a Prussian-blue screen-printed electrode	192:231	a Prussian-blue screen-printed electrode containing glucose oxidase incorporated into polyelectrolyte microcapsules (PMC)	192:312	The characteristics of an electrochemical biosensor based on a Prussian-blue screen-printed electrode containing glucose oxidase incorporated into polyelectrolyte microcapsules (PMC) are considered.
31155546	0	13	theme	Comparative	0:10	arg1	Study					12:16	Comparative Study	0:16	Comparative Study of Electrochemical Sensors	0:43	Comparative Study of Electrochemical Sensors Based on Enzyme Immobilized into Polyelectrolyte Microcapsules and into Chitosan Gel.
31155546	2	14	theme	allylamine	415:424	arg1	poly					410:413	poly	410:413	poly(allylamine hydrochloride)	410:439	PMC with the embedded enzyme were formed using sodium polystyrene sulfonate and poly(allylamine hydrochloride).
31155546	2	14	theme	allylamine	415:424	arg1	hydrochloride					426:438	allylamine hydrochloride	415:438	allylamine hydrochloride	415:438	PMC with the embedded enzyme were formed using sodium polystyrene sulfonate and poly(allylamine hydrochloride).
31155546	0	15	theme	Electrochemical	21:35	arg1	Sensors					37:43	Electrochemical Sensors	21:43	Electrochemical Sensors	21:43	Comparative Study of Electrochemical Sensors Based on Enzyme Immobilized into Polyelectrolyte Microcapsules and into Chitosan Gel.
31155546	8	16	theme	standard	1070:1077	arg1	%					1116:1116	0.92 - 0.95%	1105:1116	%	1116:1116	The biosensors' data on the glucose contents were shown to have a high correlation with the standard spectrophotometric assay (0.92 - 0.95%), which implies a possible application of the fabricated biosensors in foodstuff analysis.
31155546	8	16	theme	standard	1070:1077	arg1	assay					1098:1102	the standard spectrophotometric assay	1066:1102	the standard spectrophotometric assay (0.92 - 0.95%)	1066:1117	The biosensors' data on the glucose contents were shown to have a high correlation with the standard spectrophotometric assay (0.92 - 0.95%), which implies a possible application of the fabricated biosensors in foodstuff analysis.
31155546	7	17	theme	commercial	959:968	arg1	juices					970:975	commercial juices	959:975	commercial juices	959:975	The developed biosensors were used to assay commercial juices.
31155546	2	18	theme	embedded	343:350	arg1	enzyme					352:357	the embedded enzyme	339:357	the embedded enzyme	339:357	PMC with the embedded enzyme were formed using sodium polystyrene sulfonate and poly(allylamine hydrochloride).
31155546	8	19	with	correlation	1049:1059	arg1	%					1116:1116	0.92 - 0.95%	1105:1116	%	1116:1116	The biosensors' data on the glucose contents were shown to have a high correlation with the standard spectrophotometric assay (0.92 - 0.95%), which implies a possible application of the fabricated biosensors in foodstuff analysis.
31155546	8	19	with	correlation	1049:1059	arg1	assay					1098:1102	the standard spectrophotometric assay	1066:1102	the standard spectrophotometric assay (0.92 - 0.95%)	1066:1117	The biosensors' data on the glucose contents were shown to have a high correlation with the standard spectrophotometric assay (0.92 - 0.95%), which implies a possible application of the fabricated biosensors in foodstuff analysis.
31155546	4	20	theme	long-term	672:680	arg1	stabilities					682:692	the operational and long-term stabilities	652:692	the operational and long-term stabilities	652:692	We assessed the dependences of biosensor signals on the composition of the buffer solution, on the glucose concentration; the operational and long-term stabilities.
31155546	8	21	theme	fabricated	1164:1173	arg1	biosensors					1175:1184	the fabricated biosensors	1160:1184	the fabricated biosensors	1160:1184	The biosensors' data on the glucose contents were shown to have a high correlation with the standard spectrophotometric assay (0.92 - 0.95%), which implies a possible application of the fabricated biosensors in foodstuff analysis.
31155546	4	22	theme	solution	612:619	arg1	composition					586:596	the composition	582:596	the composition of the buffer solution	582:619	We assessed the dependences of biosensor signals on the composition of the buffer solution, on the glucose concentration; the operational and long-term stabilities.
31155546	7	23	used	used	945:948	arg2	biosensors					929:938	The developed biosensors	915:938	The developed biosensors	915:938	The developed biosensors were used to assay commercial juices.
31155546	3	24	theme	chitosan	516:523	arg1	gel					525:527	chitosan gel	516:527	chitosan gel	516:527	The characteristics were compared with those of the enzyme immobilized in chitosan gel.
31155546	4	25	from	composition	586:596	arg1	concentration					637:649	the glucose concentration	625:649	the glucose concentration	625:649	We assessed the dependences of biosensor signals on the composition of the buffer solution, on the glucose concentration; the operational and long-term stabilities.
31155546	4	26	theme	buffer	605:610	arg1	solution					612:619	the buffer solution	601:619	the buffer solution	601:619	We assessed the dependences of biosensor signals on the composition of the buffer solution, on the glucose concentration; the operational and long-term stabilities.
31155546	5	27	from	maximum	777:783	arg1	sensitive					743:751	sensitive	743:751	sensitive	743:751	The enzyme immobilized in PMC proved to be more sensitive to buffer molarity at a maximum within 35 - 40 mM.
31155546	5	27	from	maximum	777:783	arg1	enzyme					699:704	The enzyme	695:704	The enzyme immobilized in PMC	695:723	The enzyme immobilized in PMC proved to be more sensitive to buffer molarity at a maximum within 35 - 40 mM.
31155546	1	28	theme	screen-printed	208:221	arg1	electrode					223:231	a Prussian-blue screen-printed electrode	192:231	a Prussian-blue screen-printed electrode containing glucose oxidase incorporated into polyelectrolyte microcapsules (PMC)	192:312	The characteristics of an electrochemical biosensor based on a Prussian-blue screen-printed electrode containing glucose oxidase incorporated into polyelectrolyte microcapsules (PMC) are considered.
31155546	1	29	theme	glucose	244:250	arg1	oxidase					252:258	glucose oxidase	244:258	glucose oxidase incorporated into polyelectrolyte microcapsules (PMC)	244:312	The characteristics of an electrochemical biosensor based on a Prussian-blue screen-printed electrode containing glucose oxidase incorporated into polyelectrolyte microcapsules (PMC) are considered.
31155546	0	30	theme	Sensors	37:43	arg1	Study					12:16	Comparative Study	0:16	Comparative Study of Electrochemical Sensors	0:43	Comparative Study of Electrochemical Sensors Based on Enzyme Immobilized into Polyelectrolyte Microcapsules and into Chitosan Gel.
31155546	5	31	theme	buffer	756:761	arg1	molarity					763:770	buffer molarity	756:770	buffer molarity	756:770	The enzyme immobilized in PMC proved to be more sensitive to buffer molarity at a maximum within 35 - 40 mM.
31155546	4	32	theme	operational	656:666	arg1	stabilities					682:692	the operational and long-term stabilities	652:692	the operational and long-term stabilities	652:692	We assessed the dependences of biosensor signals on the composition of the buffer solution, on the glucose concentration; the operational and long-term stabilities.
31155546	8	33	theme	possible	1136:1143	arg1	application					1145:1155	a possible application	1134:1155	a possible application of the fabricated biosensors	1134:1184	The biosensors' data on the glucose contents were shown to have a high correlation with the standard spectrophotometric assay (0.92 - 0.95%), which implies a possible application of the fabricated biosensors in foodstuff analysis.
31155546	4	34	theme	signals	571:577	arg1	dependences					546:556	the dependences	542:556	the dependences of biosensor signals	542:577	We assessed the dependences of biosensor signals on the composition of the buffer solution, on the glucose concentration; the operational and long-term stabilities.
31155546	8	35	theme	0.92	1105:1108	arg1	%					1116:1116	0.92 - 0.95%	1105:1116	%	1116:1116	The biosensors' data on the glucose contents were shown to have a high correlation with the standard spectrophotometric assay (0.92 - 0.95%), which implies a possible application of the fabricated biosensors in foodstuff analysis.
31155546	8	35	theme	0.92	1105:1108	arg1	assay					1098:1102	the standard spectrophotometric assay	1066:1102	the standard spectrophotometric assay (0.92 - 0.95%)	1066:1117	The biosensors' data on the glucose contents were shown to have a high correlation with the standard spectrophotometric assay (0.92 - 0.95%), which implies a possible application of the fabricated biosensors in foodstuff analysis.
31155546	2	36	theme	polystyrene	384:394	arg1	sulfonate					396:404	sodium polystyrene sulfonate	377:404	sodium polystyrene sulfonate	377:404	PMC with the embedded enzyme were formed using sodium polystyrene sulfonate and poly(allylamine hydrochloride).
31155546	6	37	from	mM	854:855	arg1	chitosan					897:904	chitosan	897:904	chitosan	897:904	The apparent Michaelis constants were 1.5 and 4.1 mM at the immobilization in, respectively, chitosan and PMC.
31155546	6	37	from	mM	854:855	arg1	PMC					910:912	PMC	910:912	PMC	910:912	The apparent Michaelis constants were 1.5 and 4.1 mM at the immobilization in, respectively, chitosan and PMC.
31155546	2	38	theme	sodium	377:382	arg1	sulfonate					396:404	sodium polystyrene sulfonate	377:404	sodium polystyrene sulfonate	377:404	PMC with the embedded enzyme were formed using sodium polystyrene sulfonate and poly(allylamine hydrochloride).
31155546	8	39	theme	glucose	1006:1012	arg1	contents					1014:1021	the glucose contents	1002:1021	the glucose contents	1002:1021	The biosensors' data on the glucose contents were shown to have a high correlation with the standard spectrophotometric assay (0.92 - 0.95%), which implies a possible application of the fabricated biosensors in foodstuff analysis.
31155546	8	40	theme	0.95	1112:1115	arg1	%					1116:1116	0.92 - 0.95%	1105:1116	%	1116:1116	The biosensors' data on the glucose contents were shown to have a high correlation with the standard spectrophotometric assay (0.92 - 0.95%), which implies a possible application of the fabricated biosensors in foodstuff analysis.
31155546	8	40	theme	0.95	1112:1115	arg1	assay					1098:1102	the standard spectrophotometric assay	1066:1102	the standard spectrophotometric assay (0.92 - 0.95%)	1066:1117	The biosensors' data on the glucose contents were shown to have a high correlation with the standard spectrophotometric assay (0.92 - 0.95%), which implies a possible application of the fabricated biosensors in foodstuff analysis.
31155546	7	41	theme	developed	919:927	arg1	biosensors					929:938	The developed biosensors	915:938	The developed biosensors	915:938	The developed biosensors were used to assay commercial juices.
31155546	1	42	contain	containing	233:242	arg2	oxidase					252:258	glucose oxidase	244:258	glucose oxidase incorporated into polyelectrolyte microcapsules (PMC)	244:312	The characteristics of an electrochemical biosensor based on a Prussian-blue screen-printed electrode containing glucose oxidase incorporated into polyelectrolyte microcapsules (PMC) are considered.
31155546	1	42	contain	containing	233:242	arg1	electrode					223:231	a Prussian-blue screen-printed electrode	192:231	a Prussian-blue screen-printed electrode containing glucose oxidase incorporated into polyelectrolyte microcapsules (PMC)	192:312	The characteristics of an electrochemical biosensor based on a Prussian-blue screen-printed electrode containing glucose oxidase incorporated into polyelectrolyte microcapsules (PMC) are considered.
31155546	1	43	theme	polyelectrolyte	278:292	arg1	PMC					309:311	PMC	309:311	PMC	309:311	The characteristics of an electrochemical biosensor based on a Prussian-blue screen-printed electrode containing glucose oxidase incorporated into polyelectrolyte microcapsules (PMC) are considered.
31155546	1	43	theme	polyelectrolyte	278:292	arg1	microcapsules					294:306	polyelectrolyte microcapsules	278:306	polyelectrolyte microcapsules (PMC)	278:312	The characteristics of an electrochemical biosensor based on a Prussian-blue screen-printed electrode containing glucose oxidase incorporated into polyelectrolyte microcapsules (PMC) are considered.
31155546	1	44	theme	electrochemical	157:171	arg1	biosensor					173:181	an electrochemical biosensor	154:181	an electrochemical biosensor based on a Prussian-blue screen-printed electrode containing glucose oxidase incorporated into polyelectrolyte microcapsules (PMC)	154:312	The characteristics of an electrochemical biosensor based on a Prussian-blue screen-printed electrode containing glucose oxidase incorporated into polyelectrolyte microcapsules (PMC) are considered.
31155546	8	45	theme	spectrophotometric	1079:1096	arg1	%					1116:1116	0.92 - 0.95%	1105:1116	%	1116:1116	The biosensors' data on the glucose contents were shown to have a high correlation with the standard spectrophotometric assay (0.92 - 0.95%), which implies a possible application of the fabricated biosensors in foodstuff analysis.
31155546	8	45	theme	spectrophotometric	1079:1096	arg1	assay					1098:1102	the standard spectrophotometric assay	1066:1102	the standard spectrophotometric assay (0.92 - 0.95%)	1066:1117	The biosensors' data on the glucose contents were shown to have a high correlation with the standard spectrophotometric assay (0.92 - 0.95%), which implies a possible application of the fabricated biosensors in foodstuff analysis.
31155546	2	46	with	PMC	330:332	arg1	enzyme					352:357	the embedded enzyme	339:357	the embedded enzyme	339:357	PMC with the embedded enzyme were formed using sodium polystyrene sulfonate and poly(allylamine hydrochloride).
29546462	9	0	contain	containing	1051:1060	arg1	copolymer					1041:1049	a water-soluble copolymer	1025:1049	a water-soluble copolymer containing photo cross-linkable moieties	1025:1090	Additionally, this work reports the use in the composition of a water-soluble copolymer containing photo cross-linkable moieties to produce insoluble materials post-electrospinning.
29546462	9	0	contain	containing	1051:1060	arg2	moieties					1083:1090	photo cross-linkable moieties	1062:1090	photo cross-linkable moieties	1062:1090	Additionally, this work reports the use in the composition of a water-soluble copolymer containing photo cross-linkable moieties to produce insoluble materials post-electrospinning.
29546462	9	1	theme	insoluble	1103:1111	arg1	materials					1113:1121	insoluble materials	1103:1121	insoluble materials post-electrospinning	1103:1142	Additionally, this work reports the use in the composition of a water-soluble copolymer containing photo cross-linkable moieties to produce insoluble materials post-electrospinning.
29546462	8	2	theme	longitudinal	939:950	arg1	direction					952:960	the longitudinal direction	935:960	the longitudinal direction	935:960	Particularly, this reinforcement was achieved at the longitudinal direction.
29546462	0	3	theme	healthcare	87:96	arg1	applications					98:109	potential healthcare applications	77:109	potential healthcare applications	77:109	Aligned nanofibres made of poly(3-hydroxybutyrate) grafted to hyaluronan for potential healthcare applications.
29546462	6	4	theme	hairy	740:744	arg1	topography					746:755	an unusual hairy topography	729:755	an unusual hairy topography	729:755	At the nanoscale, the hybrid copolymer exhibited an unusual hairy topography.
29546462	0	5	theme	potential	77:85	arg1	applications					98:109	potential healthcare applications	77:109	potential healthcare applications	77:109	Aligned nanofibres made of poly(3-hydroxybutyrate) grafted to hyaluronan for potential healthcare applications.
29546462	6	6	theme	unusual	732:738	arg1	topography					746:755	an unusual hairy topography	729:755	an unusual hairy topography	729:755	At the nanoscale, the hybrid copolymer exhibited an unusual hairy topography.
29546462	7	7	theme	tensile	785:791	arg1	properties					793:802	tensile properties	785:802	tensile properties	785:802	Moreover, the hardness and tensile properties of the hybrid were found to be superior compared to fibres made of unmodified HA.
29546462	7	8	theme	unmodified	871:880	arg1	HA					882:883	unmodified HA	871:883	unmodified HA	871:883	Moreover, the hardness and tensile properties of the hybrid were found to be superior compared to fibres made of unmodified HA.
29546462	2	9	theme	green	321:325	arg1	process					343:349	a green electrospinning process	319:349	a green electrospinning process	319:349	Once formed, the P(3HB)-g-HA copolymer was soluble in water allowing a green electrospinning process.
29546462	10	10	theme	polymers	1238:1245	arg1	biocompatibility					1206:1221	the biocompatibility	1202:1221	the biocompatibility of the natural polymers used for the fabrication	1202:1270	The derivatives as well as their nanofibrous mats retain the biocompatibility of the natural polymers used for the fabrication.
29546462	2	11	theme	electrospinning	327:341	arg1	process					343:349	a green electrospinning process	319:349	a green electrospinning process	319:349	Once formed, the P(3HB)-g-HA copolymer was soluble in water allowing a green electrospinning process.
29546462	5	12	theme	hydrophobic	566:576	arg1	modification					578:589	The hydrophobic modification	562:589	The hydrophobic modification	562:589	The hydrophobic modification has not only increased the fibre diameter, but also the obtained layers were homogenous.
29546462	2	13	from	soluble	293:299	arg1	water					304:308	water	304:308	water allowing a green electrospinning process	304:349	Once formed, the P(3HB)-g-HA copolymer was soluble in water allowing a green electrospinning process.
29546462	10	14	theme	natural	1230:1236	arg1	polymers					1238:1245	the natural polymers	1226:1245	the natural polymers used for the fabrication	1226:1270	The derivatives as well as their nanofibrous mats retain the biocompatibility of the natural polymers used for the fabrication.
29546462	3	15	theme	nanofibres	369:378	arg1	diameters					356:364	The diameters	352:364	The diameters of nanofibres	352:378	The diameters of nanofibres can be tailored by simply varying the Mw of polymer.
29546462	0	16	theme	Aligned	0:6	arg1	nanofibres					8:17	Aligned nanofibres	0:17	Aligned nanofibres made of poly(3-hydroxybutyrate)	0:49	Aligned nanofibres made of poly(3-hydroxybutyrate) grafted to hyaluronan for potential healthcare applications.
29546462	6	17	theme	hybrid	702:707	arg1	copolymer					709:717	the hybrid copolymer	698:717	the hybrid copolymer	698:717	At the nanoscale, the hybrid copolymer exhibited an unusual hairy topography.
29546462	2	18	from	water	304:308	arg1	copolymer					279:287	the P(3HB)-g-HA copolymer	263:287	the P(3HB)-g-HA copolymer	263:287	Once formed, the P(3HB)-g-HA copolymer was soluble in water allowing a green electrospinning process.
29546462	2	18	from	water	304:308	arg1	soluble					293:299	soluble	293:299	soluble	293:299	Once formed, the P(3HB)-g-HA copolymer was soluble in water allowing a green electrospinning process.
29546462	9	19	theme	post-electrospinning	1123:1142	arg1	materials					1113:1121	insoluble materials	1103:1121	insoluble materials post-electrospinning	1103:1142	Additionally, this work reports the use in the composition of a water-soluble copolymer containing photo cross-linkable moieties to produce insoluble materials post-electrospinning.
29546462	4	20	theme	diameter	502:509	arg1	fibres					484:489	fibres	484:489	fibres of average diameter	484:509	The optimization of the process allowed to produce fibres of average diameter in the range of 100-150 nm and low polydispersity.
29546462	4	21	theme	average	494:500	arg1	diameter					502:509	average diameter	494:509	average diameter	494:509	The optimization of the process allowed to produce fibres of average diameter in the range of 100-150 nm and low polydispersity.
29546462	9	22	theme	water-soluble	1027:1039	arg1	copolymer					1041:1049	a water-soluble copolymer	1025:1049	a water-soluble copolymer containing photo cross-linkable moieties	1025:1090	Additionally, this work reports the use in the composition of a water-soluble copolymer containing photo cross-linkable moieties to produce insoluble materials post-electrospinning.
29546462	5	23	theme	obtained	647:654	arg1	homogenous					668:677	homogenous	668:677	homogenous	668:677	The hydrophobic modification has not only increased the fibre diameter, but also the obtained layers were homogenous.
29546462	5	23	theme	obtained	647:654	arg1	layers					656:661	the obtained layers	643:661	the obtained layers	643:661	The hydrophobic modification has not only increased the fibre diameter, but also the obtained layers were homogenous.
29546462	7	24	dep	hardness	772:779	arg1	the					768:770	the	768:770	the	768:770	Moreover, the hardness and tensile properties of the hybrid were found to be superior compared to fibres made of unmodified HA.
29546462	1	25	theme	hyaluronic	194:203	arg1	HA					211:212	HA	211:212	HA	211:212	In this work, a hybrid copolymer consisting of poly(3-hydroxybutyrate) grafted to hyaluronic acid (HA) was synthesised and characterised.
29546462	1	25	theme	hyaluronic	194:203	arg1	acid					205:208	hyaluronic acid	194:208	hyaluronic acid (HA)	194:213	In this work, a hybrid copolymer consisting of poly(3-hydroxybutyrate) grafted to hyaluronic acid (HA) was synthesised and characterised.
29546462	4	26	theme	process	457:463	arg1	optimization					437:448	The optimization	433:448	The optimization of the process	433:463	The optimization of the process allowed to produce fibres of average diameter in the range of 100-150 nm and low polydispersity.
29546462	3	27	theme	polymer	424:430	arg1	Mw					418:419	the Mw	414:419	the Mw of polymer	414:430	The diameters of nanofibres can be tailored by simply varying the Mw of polymer.
29546462	5	28	theme	fibre	618:622	arg1	diameter					624:631	the fibre diameter	614:631	the fibre diameter	614:631	The hydrophobic modification has not only increased the fibre diameter, but also the obtained layers were homogenous.
29546462	9	29	theme	photo	1062:1066	arg1	moieties					1083:1090	photo cross-linkable moieties	1062:1090	photo cross-linkable moieties	1062:1090	Additionally, this work reports the use in the composition of a water-soluble copolymer containing photo cross-linkable moieties to produce insoluble materials post-electrospinning.
29546462	9	30	theme	cross-linkable	1068:1081	arg1	moieties					1083:1090	photo cross-linkable moieties	1062:1090	photo cross-linkable moieties	1062:1090	Additionally, this work reports the use in the composition of a water-soluble copolymer containing photo cross-linkable moieties to produce insoluble materials post-electrospinning.
29546462	2	31	theme	-g-HA	273:277	arg1	copolymer					279:287	the P(3HB)-g-HA copolymer	263:287	the P(3HB)-g-HA copolymer	263:287	Once formed, the P(3HB)-g-HA copolymer was soluble in water allowing a green electrospinning process.
29546462	2	31	theme	-g-HA	273:277	arg1	soluble					293:299	soluble	293:299	soluble	293:299	Once formed, the P(3HB)-g-HA copolymer was soluble in water allowing a green electrospinning process.
29546462	9	32	theme	copolymer	1041:1049	arg1	composition					1010:1020	the composition	1006:1020	the composition of a water-soluble copolymer containing photo cross-linkable moieties	1006:1090	Additionally, this work reports the use in the composition of a water-soluble copolymer containing photo cross-linkable moieties to produce insoluble materials post-electrospinning.
29546462	10	33	theme	nanofibrous	1178:1188	arg1	mats					1190:1193	their nanofibrous mats	1172:1193	The derivatives as well as their nanofibrous mats	1145:1193	The derivatives as well as their nanofibrous mats retain the biocompatibility of the natural polymers used for the fabrication.
29546462	4	34	theme	100-150 nm	527:536	arg1	polydispersity					546:559	100-150 nm and low polydispersity	527:559	100-150 nm and low polydispersity	527:559	The optimization of the process allowed to produce fibres of average diameter in the range of 100-150 nm and low polydispersity.
29546462	4	35	theme	polydispersity	546:559	arg1	range					518:522	the range	514:522	the range of 100-150 nm and low polydispersity	514:559	The optimization of the process allowed to produce fibres of average diameter in the range of 100-150 nm and low polydispersity.
29546462	9	36	from	use	999:1001	arg1	composition					1010:1020	the composition	1006:1020	the composition of a water-soluble copolymer containing photo cross-linkable moieties	1006:1090	Additionally, this work reports the use in the composition of a water-soluble copolymer containing photo cross-linkable moieties to produce insoluble materials post-electrospinning.
29546462	1	37	theme	hybrid	128:133	arg1	copolymer					135:143	a hybrid copolymer	126:143	a hybrid copolymer consisting of poly(3-hydroxybutyrate) grafted to hyaluronic acid (HA)	126:213	In this work, a hybrid copolymer consisting of poly(3-hydroxybutyrate) grafted to hyaluronic acid (HA) was synthesised and characterised.
29546462	7	38	theme	hybrid	811:816	arg1	hardness					772:779	hardness	772:779	hardness	772:779	Moreover, the hardness and tensile properties of the hybrid were found to be superior compared to fibres made of unmodified HA.
29546462	7	38	theme	hybrid	811:816	arg1	properties					793:802	tensile properties	785:802	tensile properties	785:802	Moreover, the hardness and tensile properties of the hybrid were found to be superior compared to fibres made of unmodified HA.
29546462	4	39	theme	low	542:544	arg1	polydispersity					546:559	100-150 nm and low polydispersity	527:559	100-150 nm and low polydispersity	527:559	The optimization of the process allowed to produce fibres of average diameter in the range of 100-150 nm and low polydispersity.
29408017	6	0	theme	methanogens	1215:1225	arg1	abundances					1195:1204	relative abundances	1186:1204	relative abundances of total methanogens	1186:1225	However, relative abundances of total methanogens did not differ between tannin treatments.
29408017	1	1	theme	tannins	252:258	arg1	effect					199:204	the methane-decreasing effect	176:204	the methane-decreasing effect of monensin (∼21%) and different hydrolysable tannins (24%-65%) during in vitro fermentation of grass silage	176:313	This study examined whether the methane-decreasing effect of monensin (∼21%) and different hydrolysable tannins (24%-65%) during in vitro fermentation of grass silage was accompanied by changes in abundances of cellulolytics and methanogens.
29408017	11	2	theme	different	2049:2057	arg1	impact					2059:2064	a different impact	2047:2064	a different impact of monensin and tannins on ruminal cellulolytics	2047:2113	Our results demonstrate a different impact of monensin and tannins on ruminal cellulolytics and gave indication that methane decrease by monensin and chestnut tannins was associated with decreased abundances of M. ruminantium and M. stadtmanae.
29408017	7	3	theme	grape	1349:1353	arg1	tannins					1360:1366	grape seed tannins	1349:1366	grape seed tannins	1349:1366	Thus, the decreased methane production by 65% and 24% observed for chestnut and grape seed tannins, respectively, may have been caused by a lower total number of methanogens, but methane production seemed to be also dependent on changes in the microbial community composition.
29408017	4	4	theme	cells	999:1003	arg1	numbers					976:982	absolute numbers	967:982	absolute numbers of prokaryotic cells with monensin treatment	967:1027	Results revealed no change in absolute numbers of prokaryotic cells with monensin treatment, neither in LAM nor in SAM.
29408017	10	5	theme	Methanomicrobium	1883:1898	arg1	mobile					1900:1905	Methanomicrobium mobile	1883:1905	Methanomicrobium mobile	1883:1905	Proportions of Methanomicrobium mobile were decreased by monensin in LAM while chestnut and valonea had an increasing effect on this methanogenic species.
29408017	6	6	theme	relative	1186:1193	arg1	abundances					1195:1204	relative abundances	1186:1204	relative abundances of total methanogens	1186:1225	However, relative abundances of total methanogens did not differ between tannin treatments.
29408017	8	7	theme	chestnut	1628:1635	arg1	tannins					1649:1655	chestnut and valonea tannins	1628:1655	chestnut and valonea tannins	1628:1655	While the relative abundance of F. succinogenes decreased with monensin addition, chestnut and valonea tannins inhibited R. albus.
29408017	9	8	theme	tannins	1859:1865	arg1	supplementation					1819:1833	supplementation	1819:1833	supplementation of monensin or chestnut tannins	1819:1865	Moreover, a decline in relative abundances of Methanobrevibacter sp., especially M. ruminantium, and Methanosphaera stadtmanae was shown with supplementation of monensin or chestnut tannins.
29408017	8	9	theme	valonea	1641:1647	arg1	tannins					1649:1655	chestnut and valonea tannins	1628:1655	chestnut and valonea tannins	1628:1655	While the relative abundance of F. succinogenes decreased with monensin addition, chestnut and valonea tannins inhibited R. albus.
29408017	0	10	theme	cellulolytics	108:120	arg1	populations					85:95	populations	85:95	populations of ruminal cellulolytics and methanogens in vitro	85:145	Effect of the ionophore monensin and tannin extracts supplemented to grass silage on populations of ruminal cellulolytics and methanogens in vitro.
29408017	1	11	theme	monensin	209:216	arg1	effect					199:204	the methane-decreasing effect	176:204	the methane-decreasing effect of monensin (∼21%) and different hydrolysable tannins (24%-65%) during in vitro fermentation of grass silage	176:313	This study examined whether the methane-decreasing effect of monensin (∼21%) and different hydrolysable tannins (24%-65%) during in vitro fermentation of grass silage was accompanied by changes in abundances of cellulolytics and methanogens.
29408017	7	12	theme	community	1523:1531	arg1	composition					1533:1543	the microbial community composition	1509:1543	the microbial community composition	1509:1543	Thus, the decreased methane production by 65% and 24% observed for chestnut and grape seed tannins, respectively, may have been caused by a lower total number of methanogens, but methane production seemed to be also dependent on changes in the microbial community composition.
29408017	5	13	theme	total	1131:1135	arg1	counts					1149:1154	total prokaryotic counts	1131:1154	total prokaryotic counts	1131:1154	By contrast, supplementation of chestnut and grape seed tannins decreased total prokaryotic counts compared to control.
29408017	3	14	theme	DAPI	764:767	arg1	staining					770:777	4',6-diamidino-2-phenylindol (DAPI) staining	734:777	staining	770:777	Total prokaryotes were quantified by 4',6-diamidino-2-phenylindol (DAPI) staining of paraformaldehyde-ethanol-fixed cells and relative abundances of ruminal cellulolytic and methanogenic species were assessed by real time quantitative PCR.
29408017	7	15	theme	methane	1289:1295	arg1	production					1297:1306	the decreased methane production	1275:1306	the decreased methane production	1275:1306	Thus, the decreased methane production by 65% and 24% observed for chestnut and grape seed tannins, respectively, may have been caused by a lower total number of methanogens, but methane production seemed to be also dependent on changes in the microbial community composition.
29408017	3	16	theme	Total	697:701	arg1	prokaryotes					703:713	Total prokaryotes	697:713	Total prokaryotes	697:713	Total prokaryotes were quantified by 4',6-diamidino-2-phenylindol (DAPI) staining of paraformaldehyde-ethanol-fixed cells and relative abundances of ruminal cellulolytic and methanogenic species were assessed by real time quantitative PCR.
29408017	1	17	theme	grass	302:306	arg1	silage					308:313	grass silage	302:313	grass silage	302:313	This study examined whether the methane-decreasing effect of monensin (∼21%) and different hydrolysable tannins (24%-65%) during in vitro fermentation of grass silage was accompanied by changes in abundances of cellulolytics and methanogens.
29408017	3	18	theme	paraformaldehyde-ethanol-fixed	782:811	arg1	cells					813:817	paraformaldehyde-ethanol-fixed cells	782:817	paraformaldehyde-ethanol-fixed cells	782:817	Total prokaryotes were quantified by 4',6-diamidino-2-phenylindol (DAPI) staining of paraformaldehyde-ethanol-fixed cells and relative abundances of ruminal cellulolytic and methanogenic species were assessed by real time quantitative PCR.
29408017	2	19	theme	microbes	441:448	arg1	Samples					390:396	Samples	390:396	Samples of liquid (LAM) and solid (SAM) associated microbes	390:448	Samples of liquid (LAM) and solid (SAM) associated microbes were obtained from two rumen simulation technique experiments in which grass silage was either tested in combination with monensin (0, 2 or 4 mg d-1) or with different tannin extracts from chestnut, valonea, sumac and grape seed (0 or 1.5 g d-1).
29408017	0	20	theme	grass	69:73	arg1	silage					75:80	grass silage	69:80	grass silage	69:80	Effect of the ionophore monensin and tannin extracts supplemented to grass silage on populations of ruminal cellulolytics and methanogens in vitro.
29408017	7	21	from	changes	1498:1504	arg1	composition					1533:1543	the microbial community composition	1509:1543	the microbial community composition	1509:1543	Thus, the decreased methane production by 65% and 24% observed for chestnut and grape seed tannins, respectively, may have been caused by a lower total number of methanogens, but methane production seemed to be also dependent on changes in the microbial community composition.
29408017	2	22	theme	simulation	479:488	arg1	experiments					500:510	two rumen simulation technique experiments	469:510	two rumen simulation technique experiments in which grass silage was either tested in combination with monensin (0, 2 or 4 mg d-1) or with different tannin extracts from chestnut, valonea, sumac and grape seed (0 or 1.5 g d-1)	469:694	Samples of liquid (LAM) and solid (SAM) associated microbes were obtained from two rumen simulation technique experiments in which grass silage was either tested in combination with monensin (0, 2 or 4 mg d-1) or with different tannin extracts from chestnut, valonea, sumac and grape seed (0 or 1.5 g d-1).
29408017	10	23	theme	increasing	1975:1984	arg1	effect					1986:1991	an increasing effect	1972:1991	an increasing effect on this methanogenic species	1972:2020	Proportions of Methanomicrobium mobile were decreased by monensin in LAM while chestnut and valonea had an increasing effect on this methanogenic species.
29408017	7	24	theme	methane	1448:1454	arg1	production					1456:1465	methane production	1448:1465	methane production	1448:1465	Thus, the decreased methane production by 65% and 24% observed for chestnut and grape seed tannins, respectively, may have been caused by a lower total number of methanogens, but methane production seemed to be also dependent on changes in the microbial community composition.
29408017	11	25	theme	M. ruminantium	2234:2247	arg1	abundances					2220:2229	decreased abundances	2210:2229	decreased abundances of M. ruminantium and M. stadtmanae	2210:2265	Our results demonstrate a different impact of monensin and tannins on ruminal cellulolytics and gave indication that methane decrease by monensin and chestnut tannins was associated with decreased abundances of M. ruminantium and M. stadtmanae.
29408017	10	26	contain	had	1968:1970	arg1	valonea					1960:1966	valonea	1960:1966	valonea	1960:1966	Proportions of Methanomicrobium mobile were decreased by monensin in LAM while chestnut and valonea had an increasing effect on this methanogenic species.
29408017	10	26	contain	had	1968:1970	arg1	chestnut					1947:1954	chestnut	1947:1954	chestnut	1947:1954	Proportions of Methanomicrobium mobile were decreased by monensin in LAM while chestnut and valonea had an increasing effect on this methanogenic species.
29408017	10	26	contain	had	1968:1970	arg2	effect					1986:1991	an increasing effect	1972:1991	an increasing effect on this methanogenic species	1972:2020	Proportions of Methanomicrobium mobile were decreased by monensin in LAM while chestnut and valonea had an increasing effect on this methanogenic species.
29408017	4	27	with	cells	999:1003	arg1	monensin					1010:1017	monensin	1010:1017	monensin treatment	1010:1027	Results revealed no change in absolute numbers of prokaryotic cells with monensin treatment, neither in LAM nor in SAM.
29408017	3	28	dep	staining	770:777	arg1	6-diamidino-2-phenylindol					737:761	4',6-diamidino-2-phenylindol (DAPI) staining	734:777	6-diamidino-2-phenylindol	737:761	Total prokaryotes were quantified by 4',6-diamidino-2-phenylindol (DAPI) staining of paraformaldehyde-ethanol-fixed cells and relative abundances of ruminal cellulolytic and methanogenic species were assessed by real time quantitative PCR.
29408017	4	29	from	change	957:962	arg1	numbers					976:982	absolute numbers	967:982	absolute numbers of prokaryotic cells with monensin treatment	967:1027	Results revealed no change in absolute numbers of prokaryotic cells with monensin treatment, neither in LAM nor in SAM.
29408017	2	30	theme	different	608:616	arg1	extracts					625:632	different tannin extracts	608:632	different tannin extracts from chestnut, valonea, sumac and grape seed (0 or 1.5 g d-1)	608:694	Samples of liquid (LAM) and solid (SAM) associated microbes were obtained from two rumen simulation technique experiments in which grass silage was either tested in combination with monensin (0, 2 or 4 mg d-1) or with different tannin extracts from chestnut, valonea, sumac and grape seed (0 or 1.5 g d-1).
29408017	1	31	theme	%	263:263	arg1	%					267:267	24%-65%	261:267	24%-65%	261:267	This study examined whether the methane-decreasing effect of monensin (∼21%) and different hydrolysable tannins (24%-65%) during in vitro fermentation of grass silage was accompanied by changes in abundances of cellulolytics and methanogens.
29408017	1	31	theme	%	263:263	arg1	tannins					252:258	different hydrolysable tannins	229:258	different hydrolysable tannins (24%-65%)	229:268	This study examined whether the methane-decreasing effect of monensin (∼21%) and different hydrolysable tannins (24%-65%) during in vitro fermentation of grass silage was accompanied by changes in abundances of cellulolytics and methanogens.
29408017	9	32	theme	Methanobrevibacter	1723:1740	arg1	abundances					1709:1718	relative abundances	1700:1718	relative abundances of Methanobrevibacter sp., especially M. ruminantium, and Methanosphaera stadtmanae	1700:1802	Moreover, a decline in relative abundances of Methanobrevibacter sp., especially M. ruminantium, and Methanosphaera stadtmanae was shown with supplementation of monensin or chestnut tannins.
29408017	11	33	dep	monensin	2160:2167	arg1	tannins					2182:2188	tannins	2182:2188	tannins	2182:2188	Our results demonstrate a different impact of monensin and tannins on ruminal cellulolytics and gave indication that methane decrease by monensin and chestnut tannins was associated with decreased abundances of M. ruminantium and M. stadtmanae.
29408017	2	34	theme	liquid	401:406	arg1	microbes					441:448	liquid (LAM) and solid (SAM) associated microbes	401:448	liquid (LAM) and solid (SAM) associated microbes	401:448	Samples of liquid (LAM) and solid (SAM) associated microbes were obtained from two rumen simulation technique experiments in which grass silage was either tested in combination with monensin (0, 2 or 4 mg d-1) or with different tannin extracts from chestnut, valonea, sumac and grape seed (0 or 1.5 g d-1).
29408017	1	35	from	changes	334:340	arg1	abundances					345:354	abundances	345:354	abundances of cellulolytics and methanogens	345:387	This study examined whether the methane-decreasing effect of monensin (∼21%) and different hydrolysable tannins (24%-65%) during in vitro fermentation of grass silage was accompanied by changes in abundances of cellulolytics and methanogens.
29408017	5	36	theme	seed	1108:1111	arg1	tannins					1113:1119	grape seed tannins	1102:1119	grape seed tannins	1102:1119	By contrast, supplementation of chestnut and grape seed tannins decreased total prokaryotic counts compared to control.
29408017	7	37	theme	methanogens	1431:1441	arg1	number					1421:1426	a lower total number	1407:1426	a lower total number of methanogens	1407:1441	Thus, the decreased methane production by 65% and 24% observed for chestnut and grape seed tannins, respectively, may have been caused by a lower total number of methanogens, but methane production seemed to be also dependent on changes in the microbial community composition.
29408017	2	38	dep	chestnut	639:646	arg1	1.5 g d-1					685:693	1.5 g d-1	685:693	1.5 g d-1	685:693	Samples of liquid (LAM) and solid (SAM) associated microbes were obtained from two rumen simulation technique experiments in which grass silage was either tested in combination with monensin (0, 2 or 4 mg d-1) or with different tannin extracts from chestnut, valonea, sumac and grape seed (0 or 1.5 g d-1).
29408017	2	38	dep	chestnut	639:646	arg1	0					680:680	0	680:680	0	680:680	Samples of liquid (LAM) and solid (SAM) associated microbes were obtained from two rumen simulation technique experiments in which grass silage was either tested in combination with monensin (0, 2 or 4 mg d-1) or with different tannin extracts from chestnut, valonea, sumac and grape seed (0 or 1.5 g d-1).
29408017	3	39	theme	ruminal	846:852	arg1	species					884:890	ruminal cellulolytic and methanogenic species	846:890	ruminal cellulolytic and methanogenic species	846:890	Total prokaryotes were quantified by 4',6-diamidino-2-phenylindol (DAPI) staining of paraformaldehyde-ethanol-fixed cells and relative abundances of ruminal cellulolytic and methanogenic species were assessed by real time quantitative PCR.
29408017	2	40	from	seed	674:677	arg1	extracts					625:632	different tannin extracts	608:632	different tannin extracts from chestnut, valonea, sumac and grape seed (0 or 1.5 g d-1)	608:694	Samples of liquid (LAM) and solid (SAM) associated microbes were obtained from two rumen simulation technique experiments in which grass silage was either tested in combination with monensin (0, 2 or 4 mg d-1) or with different tannin extracts from chestnut, valonea, sumac and grape seed (0 or 1.5 g d-1).
29408017	0	41	theme	monensin	24:31	arg1	Effect					0:5	Effect	0:5	Effect of the ionophore monensin	0:31	Effect of the ionophore monensin and tannin extracts supplemented to grass silage on populations of ruminal cellulolytics and methanogens in vitro.
29408017	0	41	theme	monensin	24:31	arg1	extracts					44:51	tannin extracts	37:51	tannin extracts	37:51	Effect of the ionophore monensin and tannin extracts supplemented to grass silage on populations of ruminal cellulolytics and methanogens in vitro.
29408017	9	42	theme	M. ruminantium	1758:1771	arg1	abundances					1709:1718	relative abundances	1700:1718	relative abundances of Methanobrevibacter sp., especially M. ruminantium, and Methanosphaera stadtmanae	1700:1802	Moreover, a decline in relative abundances of Methanobrevibacter sp., especially M. ruminantium, and Methanosphaera stadtmanae was shown with supplementation of monensin or chestnut tannins.
29408017	2	43	dep	liquid	401:406	arg1	SAM					425:427	SAM	425:427	SAM	425:427	Samples of liquid (LAM) and solid (SAM) associated microbes were obtained from two rumen simulation technique experiments in which grass silage was either tested in combination with monensin (0, 2 or 4 mg d-1) or with different tannin extracts from chestnut, valonea, sumac and grape seed (0 or 1.5 g d-1).
29408017	2	43	dep	liquid	401:406	arg1	LAM					409:411	LAM	409:411	LAM	409:411	Samples of liquid (LAM) and solid (SAM) associated microbes were obtained from two rumen simulation technique experiments in which grass silage was either tested in combination with monensin (0, 2 or 4 mg d-1) or with different tannin extracts from chestnut, valonea, sumac and grape seed (0 or 1.5 g d-1).
29408017	0	44	theme	tannin	37:42	arg1	extracts					44:51	tannin extracts	37:51	tannin extracts	37:51	Effect of the ionophore monensin and tannin extracts supplemented to grass silage on populations of ruminal cellulolytics and methanogens in vitro.
29408017	7	45	theme	lower	1409:1413	arg1	number					1421:1426	a lower total number	1407:1426	a lower total number of methanogens	1407:1441	Thus, the decreased methane production by 65% and 24% observed for chestnut and grape seed tannins, respectively, may have been caused by a lower total number of methanogens, but methane production seemed to be also dependent on changes in the microbial community composition.
29408017	3	46	theme	species	884:890	arg1	abundances					832:841	relative abundances	823:841	relative abundances of ruminal cellulolytic and methanogenic species	823:890	Total prokaryotes were quantified by 4',6-diamidino-2-phenylindol (DAPI) staining of paraformaldehyde-ethanol-fixed cells and relative abundances of ruminal cellulolytic and methanogenic species were assessed by real time quantitative PCR.
29408017	10	47	from	effect	1986:1991	arg1	species					2014:2020	this methanogenic species	1996:2020	this methanogenic species	1996:2020	Proportions of Methanomicrobium mobile were decreased by monensin in LAM while chestnut and valonea had an increasing effect on this methanogenic species.
29408017	3	48	theme	quantitative	919:930	arg1	PCR					932:934	real time quantitative PCR	909:934	real time quantitative PCR	909:934	Total prokaryotes were quantified by 4',6-diamidino-2-phenylindol (DAPI) staining of paraformaldehyde-ethanol-fixed cells and relative abundances of ruminal cellulolytic and methanogenic species were assessed by real time quantitative PCR.
29408017	9	49	theme	stadtmanae	1793:1802	arg1	abundances					1709:1718	relative abundances	1700:1718	relative abundances of Methanobrevibacter sp., especially M. ruminantium, and Methanosphaera stadtmanae	1700:1802	Moreover, a decline in relative abundances of Methanobrevibacter sp., especially M. ruminantium, and Methanosphaera stadtmanae was shown with supplementation of monensin or chestnut tannins.
29408017	8	50	theme	relative	1556:1563	arg1	abundance					1565:1573	the relative abundance	1552:1573	the relative abundance of F. succinogenes	1552:1592	While the relative abundance of F. succinogenes decreased with monensin addition, chestnut and valonea tannins inhibited R. albus.
29408017	4	51	theme	prokaryotic	987:997	arg1	cells					999:1003	prokaryotic cells	987:1003	prokaryotic cells with monensin treatment	987:1027	Results revealed no change in absolute numbers of prokaryotic cells with monensin treatment, neither in LAM nor in SAM.
29408017	9	52	dep	Methanobrevibacter	1723:1740	arg1	sp.					1742:1744	Methanobrevibacter sp.	1723:1744	Methanobrevibacter sp.	1723:1744	Moreover, a decline in relative abundances of Methanobrevibacter sp., especially M. ruminantium, and Methanosphaera stadtmanae was shown with supplementation of monensin or chestnut tannins.
29408017	1	53	theme	hydrolysable	239:250	arg1	%					267:267	24%-65%	261:267	24%-65%	261:267	This study examined whether the methane-decreasing effect of monensin (∼21%) and different hydrolysable tannins (24%-65%) during in vitro fermentation of grass silage was accompanied by changes in abundances of cellulolytics and methanogens.
29408017	1	53	theme	hydrolysable	239:250	arg1	tannins					252:258	different hydrolysable tannins	229:258	different hydrolysable tannins (24%-65%)	229:268	This study examined whether the methane-decreasing effect of monensin (∼21%) and different hydrolysable tannins (24%-65%) during in vitro fermentation of grass silage was accompanied by changes in abundances of cellulolytics and methanogens.
29408017	11	54	theme	monensin	2069:2076	arg1	impact					2059:2064	a different impact	2047:2064	a different impact of monensin and tannins on ruminal cellulolytics	2047:2113	Our results demonstrate a different impact of monensin and tannins on ruminal cellulolytics and gave indication that methane decrease by monensin and chestnut tannins was associated with decreased abundances of M. ruminantium and M. stadtmanae.
29408017	6	55	theme	total	1209:1213	arg1	methanogens					1215:1225	total methanogens	1209:1225	total methanogens	1209:1225	However, relative abundances of total methanogens did not differ between tannin treatments.
29408017	5	56	theme	prokaryotic	1137:1147	arg1	counts					1149:1154	total prokaryotic counts	1131:1154	total prokaryotic counts	1131:1154	By contrast, supplementation of chestnut and grape seed tannins decreased total prokaryotic counts compared to control.
29408017	7	57	theme	seed	1355:1358	arg1	tannins					1360:1366	grape seed tannins	1349:1366	grape seed tannins	1349:1366	Thus, the decreased methane production by 65% and 24% observed for chestnut and grape seed tannins, respectively, may have been caused by a lower total number of methanogens, but methane production seemed to be also dependent on changes in the microbial community composition.
29408017	9	58	theme	monensin	1838:1845	arg1	tannins					1859:1865	monensin or chestnut tannins	1838:1865	monensin or chestnut tannins	1838:1865	Moreover, a decline in relative abundances of Methanobrevibacter sp., especially M. ruminantium, and Methanosphaera stadtmanae was shown with supplementation of monensin or chestnut tannins.
29408017	8	59	theme	monensin	1609:1616	arg1	addition					1618:1625	monensin addition	1609:1625	monensin addition	1609:1625	While the relative abundance of F. succinogenes decreased with monensin addition, chestnut and valonea tannins inhibited R. albus.
29408017	2	60	theme	solid	418:422	arg1	microbes					441:448	liquid (LAM) and solid (SAM) associated microbes	401:448	liquid (LAM) and solid (SAM) associated microbes	401:448	Samples of liquid (LAM) and solid (SAM) associated microbes were obtained from two rumen simulation technique experiments in which grass silage was either tested in combination with monensin (0, 2 or 4 mg d-1) or with different tannin extracts from chestnut, valonea, sumac and grape seed (0 or 1.5 g d-1).
29408017	11	61	theme	ruminal	2093:2099	arg1	cellulolytics					2101:2113	ruminal cellulolytics	2093:2113	ruminal cellulolytics	2093:2113	Our results demonstrate a different impact of monensin and tannins on ruminal cellulolytics and gave indication that methane decrease by monensin and chestnut tannins was associated with decreased abundances of M. ruminantium and M. stadtmanae.
29408017	9	62	theme	chestnut	1850:1857	arg1	tannins					1859:1865	monensin or chestnut tannins	1838:1865	monensin or chestnut tannins	1838:1865	Moreover, a decline in relative abundances of Methanobrevibacter sp., especially M. ruminantium, and Methanosphaera stadtmanae was shown with supplementation of monensin or chestnut tannins.
29408017	2	63	with	combination	555:565	arg1	extracts					625:632	different tannin extracts	608:632	different tannin extracts from chestnut, valonea, sumac and grape seed (0 or 1.5 g d-1)	608:694	Samples of liquid (LAM) and solid (SAM) associated microbes were obtained from two rumen simulation technique experiments in which grass silage was either tested in combination with monensin (0, 2 or 4 mg d-1) or with different tannin extracts from chestnut, valonea, sumac and grape seed (0 or 1.5 g d-1).
29408017	2	63	with	combination	555:565	arg1	monensin					572:579	monensin	572:579	monensin (0, 2 or 4 mg d-1)	572:598	Samples of liquid (LAM) and solid (SAM) associated microbes were obtained from two rumen simulation technique experiments in which grass silage was either tested in combination with monensin (0, 2 or 4 mg d-1) or with different tannin extracts from chestnut, valonea, sumac and grape seed (0 or 1.5 g d-1).
29408017	1	64	theme	methane-decreasing	180:197	arg1	effect					199:204	the methane-decreasing effect	176:204	the methane-decreasing effect of monensin (∼21%) and different hydrolysable tannins (24%-65%) during in vitro fermentation of grass silage	176:313	This study examined whether the methane-decreasing effect of monensin (∼21%) and different hydrolysable tannins (24%-65%) during in vitro fermentation of grass silage was accompanied by changes in abundances of cellulolytics and methanogens.
29408017	2	65	dep	monensin	572:579	arg1	4 mg d-1					590:597	4 mg d-1	590:597	4 mg d-1	590:597	Samples of liquid (LAM) and solid (SAM) associated microbes were obtained from two rumen simulation technique experiments in which grass silage was either tested in combination with monensin (0, 2 or 4 mg d-1) or with different tannin extracts from chestnut, valonea, sumac and grape seed (0 or 1.5 g d-1).
29408017	2	65	dep	monensin	572:579	arg1	2					585:585	2	585:585	2	585:585	Samples of liquid (LAM) and solid (SAM) associated microbes were obtained from two rumen simulation technique experiments in which grass silage was either tested in combination with monensin (0, 2 or 4 mg d-1) or with different tannin extracts from chestnut, valonea, sumac and grape seed (0 or 1.5 g d-1).
29408017	11	66	theme	tannins	2082:2088	arg1	impact					2059:2064	a different impact	2047:2064	a different impact of monensin and tannins on ruminal cellulolytics	2047:2113	Our results demonstrate a different impact of monensin and tannins on ruminal cellulolytics and gave indication that methane decrease by monensin and chestnut tannins was associated with decreased abundances of M. ruminantium and M. stadtmanae.
29408017	2	67	from	chestnut	639:646	arg1	extracts					625:632	different tannin extracts	608:632	different tannin extracts from chestnut, valonea, sumac and grape seed (0 or 1.5 g d-1)	608:694	Samples of liquid (LAM) and solid (SAM) associated microbes were obtained from two rumen simulation technique experiments in which grass silage was either tested in combination with monensin (0, 2 or 4 mg d-1) or with different tannin extracts from chestnut, valonea, sumac and grape seed (0 or 1.5 g d-1).
29408017	7	68	theme	microbial	1513:1521	arg1	composition					1533:1543	the microbial community composition	1509:1543	the microbial community composition	1509:1543	Thus, the decreased methane production by 65% and 24% observed for chestnut and grape seed tannins, respectively, may have been caused by a lower total number of methanogens, but methane production seemed to be also dependent on changes in the microbial community composition.
29408017	11	69	theme	M. stadtmanae	2253:2265	arg1	abundances					2220:2229	decreased abundances	2210:2229	decreased abundances of M. ruminantium and M. stadtmanae	2210:2265	Our results demonstrate a different impact of monensin and tannins on ruminal cellulolytics and gave indication that methane decrease by monensin and chestnut tannins was associated with decreased abundances of M. ruminantium and M. stadtmanae.
29408017	2	70	from	valonea	649:655	arg1	extracts					625:632	different tannin extracts	608:632	different tannin extracts from chestnut, valonea, sumac and grape seed (0 or 1.5 g d-1)	608:694	Samples of liquid (LAM) and solid (SAM) associated microbes were obtained from two rumen simulation technique experiments in which grass silage was either tested in combination with monensin (0, 2 or 4 mg d-1) or with different tannin extracts from chestnut, valonea, sumac and grape seed (0 or 1.5 g d-1).
29408017	10	71	theme	mobile	1900:1905	arg1	Proportions					1868:1878	Proportions	1868:1878	Proportions of Methanomicrobium mobile	1868:1905	Proportions of Methanomicrobium mobile were decreased by monensin in LAM while chestnut and valonea had an increasing effect on this methanogenic species.
29408017	1	72	theme	cellulolytics	359:371	arg1	abundances					345:354	abundances	345:354	abundances of cellulolytics and methanogens	345:387	This study examined whether the methane-decreasing effect of monensin (∼21%) and different hydrolysable tannins (24%-65%) during in vitro fermentation of grass silage was accompanied by changes in abundances of cellulolytics and methanogens.
29408017	11	73	theme	methane	2140:2146	arg1	decrease					2148:2155	methane decrease	2140:2155	methane decrease by monensin and chestnut tannins	2140:2188	Our results demonstrate a different impact of monensin and tannins on ruminal cellulolytics and gave indication that methane decrease by monensin and chestnut tannins was associated with decreased abundances of M. ruminantium and M. stadtmanae.
29408017	2	74	theme	technique	490:498	arg1	experiments					500:510	two rumen simulation technique experiments	469:510	two rumen simulation technique experiments in which grass silage was either tested in combination with monensin (0, 2 or 4 mg d-1) or with different tannin extracts from chestnut, valonea, sumac and grape seed (0 or 1.5 g d-1)	469:694	Samples of liquid (LAM) and solid (SAM) associated microbes were obtained from two rumen simulation technique experiments in which grass silage was either tested in combination with monensin (0, 2 or 4 mg d-1) or with different tannin extracts from chestnut, valonea, sumac and grape seed (0 or 1.5 g d-1).
29408017	2	75	from	sumac	658:662	arg1	extracts					625:632	different tannin extracts	608:632	different tannin extracts from chestnut, valonea, sumac and grape seed (0 or 1.5 g d-1)	608:694	Samples of liquid (LAM) and solid (SAM) associated microbes were obtained from two rumen simulation technique experiments in which grass silage was either tested in combination with monensin (0, 2 or 4 mg d-1) or with different tannin extracts from chestnut, valonea, sumac and grape seed (0 or 1.5 g d-1).
29408017	11	76	from	impact	2059:2064	arg1	cellulolytics					2101:2113	ruminal cellulolytics	2093:2113	ruminal cellulolytics	2093:2113	Our results demonstrate a different impact of monensin and tannins on ruminal cellulolytics and gave indication that methane decrease by monensin and chestnut tannins was associated with decreased abundances of M. ruminantium and M. stadtmanae.
29408017	6	77	theme	tannin	1250:1255	arg1	treatments					1257:1266	tannin treatments	1250:1266	tannin treatments	1250:1266	However, relative abundances of total methanogens did not differ between tannin treatments.
29408017	1	78	theme	methanogens	377:387	arg1	abundances					345:354	abundances	345:354	abundances of cellulolytics and methanogens	345:387	This study examined whether the methane-decreasing effect of monensin (∼21%) and different hydrolysable tannins (24%-65%) during in vitro fermentation of grass silage was accompanied by changes in abundances of cellulolytics and methanogens.
29408017	2	79	theme	grass	521:525	arg1	silage					527:532	grass silage	521:532	grass silage	521:532	Samples of liquid (LAM) and solid (SAM) associated microbes were obtained from two rumen simulation technique experiments in which grass silage was either tested in combination with monensin (0, 2 or 4 mg d-1) or with different tannin extracts from chestnut, valonea, sumac and grape seed (0 or 1.5 g d-1).
29408017	7	80	theme	decreased	1279:1287	arg1	production					1297:1306	the decreased methane production	1275:1306	the decreased methane production	1275:1306	Thus, the decreased methane production by 65% and 24% observed for chestnut and grape seed tannins, respectively, may have been caused by a lower total number of methanogens, but methane production seemed to be also dependent on changes in the microbial community composition.
29408017	11	81	theme	decreased	2210:2218	arg1	abundances					2220:2229	decreased abundances	2210:2229	decreased abundances of M. ruminantium and M. stadtmanae	2210:2265	Our results demonstrate a different impact of monensin and tannins on ruminal cellulolytics and gave indication that methane decrease by monensin and chestnut tannins was associated with decreased abundances of M. ruminantium and M. stadtmanae.
29408017	1	82	theme	silage	308:313	arg1	fermentation					286:297	in vitro fermentation	277:297	in vitro fermentation of grass silage	277:313	This study examined whether the methane-decreasing effect of monensin (∼21%) and different hydrolysable tannins (24%-65%) during in vitro fermentation of grass silage was accompanied by changes in abundances of cellulolytics and methanogens.
29408017	3	83	theme	cells	813:817	arg1	staining					770:777	4',6-diamidino-2-phenylindol (DAPI) staining	734:777	staining	770:777	Total prokaryotes were quantified by 4',6-diamidino-2-phenylindol (DAPI) staining of paraformaldehyde-ethanol-fixed cells and relative abundances of ruminal cellulolytic and methanogenic species were assessed by real time quantitative PCR.
29408017	2	84	theme	associated	430:439	arg1	microbes					441:448	liquid (LAM) and solid (SAM) associated microbes	401:448	liquid (LAM) and solid (SAM) associated microbes	401:448	Samples of liquid (LAM) and solid (SAM) associated microbes were obtained from two rumen simulation technique experiments in which grass silage was either tested in combination with monensin (0, 2 or 4 mg d-1) or with different tannin extracts from chestnut, valonea, sumac and grape seed (0 or 1.5 g d-1).
29408017	2	85	theme	rumen	473:477	arg1	experiments					500:510	two rumen simulation technique experiments	469:510	two rumen simulation technique experiments in which grass silage was either tested in combination with monensin (0, 2 or 4 mg d-1) or with different tannin extracts from chestnut, valonea, sumac and grape seed (0 or 1.5 g d-1)	469:694	Samples of liquid (LAM) and solid (SAM) associated microbes were obtained from two rumen simulation technique experiments in which grass silage was either tested in combination with monensin (0, 2 or 4 mg d-1) or with different tannin extracts from chestnut, valonea, sumac and grape seed (0 or 1.5 g d-1).
29408017	0	86	theme	ruminal	100:106	arg1	cellulolytics					108:120	ruminal cellulolytics	100:120	ruminal cellulolytics	100:120	Effect of the ionophore monensin and tannin extracts supplemented to grass silage on populations of ruminal cellulolytics and methanogens in vitro.
29408017	10	87	theme	methanogenic	2001:2012	arg1	species					2014:2020	this methanogenic species	1996:2020	this methanogenic species	1996:2020	Proportions of Methanomicrobium mobile were decreased by monensin in LAM while chestnut and valonea had an increasing effect on this methanogenic species.
29408017	9	88	theme	relative	1700:1707	arg1	abundances					1709:1718	relative abundances	1700:1718	relative abundances of Methanobrevibacter sp., especially M. ruminantium, and Methanosphaera stadtmanae	1700:1802	Moreover, a decline in relative abundances of Methanobrevibacter sp., especially M. ruminantium, and Methanosphaera stadtmanae was shown with supplementation of monensin or chestnut tannins.
29408017	2	89	theme	tannin	618:623	arg1	extracts					625:632	different tannin extracts	608:632	different tannin extracts from chestnut, valonea, sumac and grape seed (0 or 1.5 g d-1)	608:694	Samples of liquid (LAM) and solid (SAM) associated microbes were obtained from two rumen simulation technique experiments in which grass silage was either tested in combination with monensin (0, 2 or 4 mg d-1) or with different tannin extracts from chestnut, valonea, sumac and grape seed (0 or 1.5 g d-1).
29408017	3	90	theme	relative	823:830	arg1	abundances					832:841	relative abundances	823:841	relative abundances of ruminal cellulolytic and methanogenic species	823:890	Total prokaryotes were quantified by 4',6-diamidino-2-phenylindol (DAPI) staining of paraformaldehyde-ethanol-fixed cells and relative abundances of ruminal cellulolytic and methanogenic species were assessed by real time quantitative PCR.
29408017	0	91	theme	ionophore	14:22	arg1	monensin					24:31	the ionophore monensin	10:31	the ionophore monensin	10:31	Effect of the ionophore monensin and tannin extracts supplemented to grass silage on populations of ruminal cellulolytics and methanogens in vitro.
29408017	5	92	theme	grape	1102:1106	arg1	tannins					1113:1119	grape seed tannins	1102:1119	grape seed tannins	1102:1119	By contrast, supplementation of chestnut and grape seed tannins decreased total prokaryotic counts compared to control.
29408017	4	93	dep	monensin	1010:1017	arg1	treatment					1019:1027	treatment	1019:1027	monensin treatment	1010:1027	Results revealed no change in absolute numbers of prokaryotic cells with monensin treatment, neither in LAM nor in SAM.
29408017	3	94	theme	methanogenic	871:882	arg1	species					884:890	ruminal cellulolytic and methanogenic species	846:890	ruminal cellulolytic and methanogenic species	846:890	Total prokaryotes were quantified by 4',6-diamidino-2-phenylindol (DAPI) staining of paraformaldehyde-ethanol-fixed cells and relative abundances of ruminal cellulolytic and methanogenic species were assessed by real time quantitative PCR.
29408017	4	95	theme	absolute	967:974	arg1	numbers					976:982	absolute numbers	967:982	absolute numbers of prokaryotic cells with monensin treatment	967:1027	Results revealed no change in absolute numbers of prokaryotic cells with monensin treatment, neither in LAM nor in SAM.
29408017	5	96	theme	tannins	1113:1119	arg1	supplementation					1070:1084	supplementation	1070:1084	supplementation of chestnut and grape seed tannins	1070:1119	By contrast, supplementation of chestnut and grape seed tannins decreased total prokaryotic counts compared to control.
29408017	3	97	theme	cellulolytic	854:865	arg1	species					884:890	ruminal cellulolytic and methanogenic species	846:890	ruminal cellulolytic and methanogenic species	846:890	Total prokaryotes were quantified by 4',6-diamidino-2-phenylindol (DAPI) staining of paraformaldehyde-ethanol-fixed cells and relative abundances of ruminal cellulolytic and methanogenic species were assessed by real time quantitative PCR.
29408017	1	98	theme	in vitro	277:284	arg1	fermentation					286:297	in vitro fermentation	277:297	in vitro fermentation of grass silage	277:313	This study examined whether the methane-decreasing effect of monensin (∼21%) and different hydrolysable tannins (24%-65%) during in vitro fermentation of grass silage was accompanied by changes in abundances of cellulolytics and methanogens.
29408017	9	99	from	decline	1689:1695	arg1	abundances					1709:1718	relative abundances	1700:1718	relative abundances of Methanobrevibacter sp., especially M. ruminantium, and Methanosphaera stadtmanae	1700:1802	Moreover, a decline in relative abundances of Methanobrevibacter sp., especially M. ruminantium, and Methanosphaera stadtmanae was shown with supplementation of monensin or chestnut tannins.
29408017	0	100	theme	methanogens	126:136	arg1	populations					85:95	populations	85:95	populations of ruminal cellulolytics and methanogens in vitro	85:145	Effect of the ionophore monensin and tannin extracts supplemented to grass silage on populations of ruminal cellulolytics and methanogens in vitro.
29408017	5	101	theme	chestnut	1089:1096	arg1	supplementation					1070:1084	supplementation	1070:1084	supplementation of chestnut and grape seed tannins	1070:1119	By contrast, supplementation of chestnut and grape seed tannins decreased total prokaryotic counts compared to control.
29408017	7	102	theme	total	1415:1419	arg1	number					1421:1426	a lower total number	1407:1426	a lower total number of methanogens	1407:1441	Thus, the decreased methane production by 65% and 24% observed for chestnut and grape seed tannins, respectively, may have been caused by a lower total number of methanogens, but methane production seemed to be also dependent on changes in the microbial community composition.
29408017	8	103	theme	F. succinogenes	1578:1592	arg1	abundance					1565:1573	the relative abundance	1552:1573	the relative abundance of F. succinogenes	1552:1592	While the relative abundance of F. succinogenes decreased with monensin addition, chestnut and valonea tannins inhibited R. albus.
29408017	1	104	theme	different	229:237	arg1	%					267:267	24%-65%	261:267	24%-65%	261:267	This study examined whether the methane-decreasing effect of monensin (∼21%) and different hydrolysable tannins (24%-65%) during in vitro fermentation of grass silage was accompanied by changes in abundances of cellulolytics and methanogens.
29408017	1	104	theme	different	229:237	arg1	tannins					252:258	different hydrolysable tannins	229:258	different hydrolysable tannins (24%-65%)	229:268	This study examined whether the methane-decreasing effect of monensin (∼21%) and different hydrolysable tannins (24%-65%) during in vitro fermentation of grass silage was accompanied by changes in abundances of cellulolytics and methanogens.
31132147	11	0	theme	dairy	1938:1942	arg1	product					1944:1950	dairy product	1938:1950	dairy product	1938:1950	In addition, textural properties of yoghurt made from two milks were also determined, and we suggested that SHCM could be used as a new resource candidate for developing dairy product in the future.
31132147	6	1	theme	yoghurt	947:953	arg1	higher					1001:1006	higher	1001:1006	higher	1001:1006	The G' of yoghurt made from SHCM during storage time was always higher than that made from HCM, indicating that SHCM yoghurt showed higher apparent viscosity values.
31132147	6	1	theme	yoghurt	947:953	arg1	G					941:941	The G	937:941	The G' of yoghurt made from SHCM during storage time	937:988	The G' of yoghurt made from SHCM during storage time was always higher than that made from HCM, indicating that SHCM yoghurt showed higher apparent viscosity values.
31132147	11	2	theme	resource	1904:1911	arg1	SHCM					1876:1879	SHCM	1876:1879	SHCM	1876:1879	In addition, textural properties of yoghurt made from two milks were also determined, and we suggested that SHCM could be used as a new resource candidate for developing dairy product in the future.
31132147	11	2	theme	resource	1904:1911	arg1	candidate					1913:1921	a new resource candidate	1898:1921	a new resource candidate for developing dairy product in the future	1898:1964	In addition, textural properties of yoghurt made from two milks were also determined, and we suggested that SHCM could be used as a new resource candidate for developing dairy product in the future.
31132147	10	3	theme	different	1749:1757	arg1	seasons					1759:1765	different seasons	1749:1765	different seasons	1749:1765	All the data could be used to produce different dairy products using raw milk from different seasons.
31132147	8	4	from	performance	1420:1430	arg1	resistance					1443:1452	disease resistance	1435:1452	disease resistance	1435:1452	PRACTICAL APPLICATION: Compared with Chinese Holstein cattle, Simmental crossbred cattle have strong resistance to moist weather and better performance in disease resistance, which are particularly important for lactating cows persisting in south of China.
31132147	2	5	theme	milk	335:338	arg1	characterization					311:326	physicochemical characterization	295:326	physicochemical characterization of raw milk	295:338	In the experiment, physicochemical characterization of raw milk was evaluated according to the Standards.
31132147	1	6	theme	alternative	235:245	arg1	which					217:221	which	217:221	which	217:221	This study aimed to evaluate a new milk resource from Simmental crossbred cattle, which could be an alternative raw milk for dairy products.
31132147	1	6	theme	alternative	235:245	arg1	milk					251:254	an alternative raw milk	232:254	an alternative raw milk for dairy products	232:273	This study aimed to evaluate a new milk resource from Simmental crossbred cattle, which could be an alternative raw milk for dairy products.
31132147	3	7	theme	Holstein	508:515	arg1	samples					532:538	Chinese Holstein cow milk (HCM) samples	500:538	Chinese Holstein cow milk (HCM) samples	500:538	In addition, Simmental crossbred cattle milk (SHCM) samples had the higher contents of total amino acid than those of Chinese Holstein cow milk (HCM) samples, especially for Leu, Lys, Met, and Phe.
31132147	7	8	theme	products	1270:1277	arg1	resource					1252:1259	a new better resource	1239:1259	a new better resource of dairy products	1239:1277	Logically, the milk from Simmental crossbred cattle has desirable physicochemical characteristics and nutritional value, which could be a new better resource of dairy products.
31132147	7	8	theme	products	1270:1277	arg1	value					1217:1221	nutritional value	1205:1221	nutritional value	1205:1221	Logically, the milk from Simmental crossbred cattle has desirable physicochemical characteristics and nutritional value, which could be a new better resource of dairy products.
31132147	1	9	theme	new	166:168	arg1	resource					175:182	a new milk resource	164:182	a new milk resource from Simmental crossbred cattle	164:214	This study aimed to evaluate a new milk resource from Simmental crossbred cattle, which could be an alternative raw milk for dairy products.
31132147	6	10	theme	viscosity	1085:1093	arg1	values					1095:1100	higher apparent viscosity values	1069:1100	higher apparent viscosity values	1069:1100	The G' of yoghurt made from SHCM during storage time was always higher than that made from HCM, indicating that SHCM yoghurt showed higher apparent viscosity values.
31132147	3	11	theme	HCM	527:529	arg1	samples					532:538	Chinese Holstein cow milk (HCM) samples	500:538	Chinese Holstein cow milk (HCM) samples	500:538	In addition, Simmental crossbred cattle milk (SHCM) samples had the higher contents of total amino acid than those of Chinese Holstein cow milk (HCM) samples, especially for Leu, Lys, Met, and Phe.
31132147	7	12	dep	new	1241:1243	arg1	better					1245:1250	better	1245:1250	better	1245:1250	Logically, the milk from Simmental crossbred cattle has desirable physicochemical characteristics and nutritional value, which could be a new better resource of dairy products.
31132147	0	13	theme	Processed	113:121	arg1	Properties					123:132	its Physicochemical, Nutritional, and Processed Properties	75:132	its Physicochemical, Nutritional, and Processed Properties	75:132	Change in Chemical Composition of Simmental Crossbred Cattle Milk Improved its Physicochemical, Nutritional, and Processed Properties.
31132147	3	14	theme	milk	521:524	arg1	samples					532:538	Chinese Holstein cow milk (HCM) samples	500:538	Chinese Holstein cow milk (HCM) samples	500:538	In addition, Simmental crossbred cattle milk (SHCM) samples had the higher contents of total amino acid than those of Chinese Holstein cow milk (HCM) samples, especially for Leu, Lys, Met, and Phe.
31132147	9	15	theme	SHCM	1576:1579	arg1	values					1566:1571	the nutritional values	1550:1571	the nutritional values of SHCM and HCM	1550:1587	We evaluated the nutritional values of SHCM and HCM, and compared the seasonal variation on chemical composition of SHCM sample.
31132147	6	16	theme	apparent	1076:1083	arg1	values					1095:1100	higher apparent viscosity values	1069:1100	higher apparent viscosity values	1069:1100	The G' of yoghurt made from SHCM during storage time was always higher than that made from HCM, indicating that SHCM yoghurt showed higher apparent viscosity values.
31132147	7	17	contain	has	1155:1157	arg2	characteristics					1185:1199	desirable physicochemical characteristics	1159:1199	desirable physicochemical characteristics	1159:1199	Logically, the milk from Simmental crossbred cattle has desirable physicochemical characteristics and nutritional value, which could be a new better resource of dairy products.
31132147	7	17	contain	has	1155:1157	arg1	milk					1118:1121	the milk	1114:1121	the milk from Simmental crossbred cattle	1114:1153	Logically, the milk from Simmental crossbred cattle has desirable physicochemical characteristics and nutritional value, which could be a new better resource of dairy products.
31132147	7	17	contain	has	1155:1157	arg2	resource					1252:1259	a new better resource	1239:1259	a new better resource of dairy products	1239:1277	Logically, the milk from Simmental crossbred cattle has desirable physicochemical characteristics and nutritional value, which could be a new better resource of dairy products.
31132147	7	17	contain	has	1155:1157	arg2	value					1217:1221	nutritional value	1205:1221	nutritional value	1205:1221	Logically, the milk from Simmental crossbred cattle has desirable physicochemical characteristics and nutritional value, which could be a new better resource of dairy products.
31132147	9	18	theme	HCM	1585:1587	arg1	values					1566:1571	the nutritional values	1550:1571	the nutritional values of SHCM and HCM	1550:1587	We evaluated the nutritional values of SHCM and HCM, and compared the seasonal variation on chemical composition of SHCM sample.
31132147	4	19	theme	protein	770:776	arg1	contents					778:785	protein contents	770:785	protein contents	770:785	We also observed the contents of protein, fat, and lactose in SHCM samples changed during 12 months, where in milk fat content was mostly affected by the season, followed by the lactose and protein contents.
31132147	8	20	theme	Holstein	1325:1332	arg1	cattle					1334:1339	Chinese Holstein cattle	1317:1339	Chinese Holstein cattle	1317:1339	PRACTICAL APPLICATION: Compared with Chinese Holstein cattle, Simmental crossbred cattle have strong resistance to moist weather and better performance in disease resistance, which are particularly important for lactating cows persisting in south of China.
31132147	6	21	theme	SHCM	1049:1052	arg1	yoghurt					1054:1060	SHCM yoghurt	1049:1060	SHCM yoghurt	1049:1060	The G' of yoghurt made from SHCM during storage time was always higher than that made from HCM, indicating that SHCM yoghurt showed higher apparent viscosity values.
31132147	10	22	used	used	1688:1691	arg2	data					1674:1677	All the data	1666:1677	All the data	1666:1677	All the data could be used to produce different dairy products using raw milk from different seasons.
31132147	7	23	theme	nutritional	1205:1215	arg1	value					1217:1221	nutritional value	1205:1221	nutritional value	1205:1221	Logically, the milk from Simmental crossbred cattle has desirable physicochemical characteristics and nutritional value, which could be a new better resource of dairy products.
31132147	7	23	theme	nutritional	1205:1215	arg1	resource					1252:1259	a new better resource	1239:1259	a new better resource of dairy products	1239:1277	Logically, the milk from Simmental crossbred cattle has desirable physicochemical characteristics and nutritional value, which could be a new better resource of dairy products.
31132147	0	24	theme	Milk	61:64	arg1	Composition					19:29	Chemical Composition	10:29	Chemical Composition of Simmental Crossbred Cattle Milk	10:64	Change in Chemical Composition of Simmental Crossbred Cattle Milk Improved its Physicochemical, Nutritional, and Processed Properties.
31132147	9	25	theme	chemical	1629:1636	arg1	composition					1638:1648	chemical composition	1629:1648	chemical composition of SHCM sample	1629:1663	We evaluated the nutritional values of SHCM and HCM, and compared the seasonal variation on chemical composition of SHCM sample.
31132147	0	26	from	Change	0:5	arg1	Composition					19:29	Chemical Composition	10:29	Chemical Composition of Simmental Crossbred Cattle Milk	10:64	Change in Chemical Composition of Simmental Crossbred Cattle Milk Improved its Physicochemical, Nutritional, and Processed Properties.
31132147	3	27	theme	milk	422:425	arg1	samples					434:440	Simmental crossbred cattle milk (SHCM) samples	395:440	Simmental crossbred cattle milk (SHCM) samples	395:440	In addition, Simmental crossbred cattle milk (SHCM) samples had the higher contents of total amino acid than those of Chinese Holstein cow milk (HCM) samples, especially for Leu, Lys, Met, and Phe.
31132147	9	28	theme	sample	1658:1663	arg1	composition					1638:1648	chemical composition	1629:1648	chemical composition of SHCM sample	1629:1663	We evaluated the nutritional values of SHCM and HCM, and compared the seasonal variation on chemical composition of SHCM sample.
31132147	8	29	theme	moist	1395:1399	arg1	weather					1401:1407	moist weather	1395:1407	moist weather	1395:1407	PRACTICAL APPLICATION: Compared with Chinese Holstein cattle, Simmental crossbred cattle have strong resistance to moist weather and better performance in disease resistance, which are particularly important for lactating cows persisting in south of China.
31132147	4	30	theme	lactose	631:637	arg1	contents					601:608	the contents	597:608	the contents of protein, fat, and lactose in SHCM samples changed during 12 months, where in milk fat content was mostly affected by the season, followed by the lactose and protein contents	597:785	We also observed the contents of protein, fat, and lactose in SHCM samples changed during 12 months, where in milk fat content was mostly affected by the season, followed by the lactose and protein contents.
31132147	3	31	theme	crossbred	405:413	arg1	samples					434:440	Simmental crossbred cattle milk (SHCM) samples	395:440	Simmental crossbred cattle milk (SHCM) samples	395:440	In addition, Simmental crossbred cattle milk (SHCM) samples had the higher contents of total amino acid than those of Chinese Holstein cow milk (HCM) samples, especially for Leu, Lys, Met, and Phe.
31132147	5	32	theme	profile	806:812	arg1	analysis					814:821	texture profile analysis	798:821	texture profile analysis	798:821	Moreover, texture profile analysis showed that yoghurt made from SHCM had stronger structures, which especially reflected in hardness and chewiness.
31132147	7	33	theme	physicochemical	1169:1183	arg1	characteristics					1185:1199	desirable physicochemical characteristics	1159:1199	desirable physicochemical characteristics	1159:1199	Logically, the milk from Simmental crossbred cattle has desirable physicochemical characteristics and nutritional value, which could be a new better resource of dairy products.
31132147	4	34	theme	SHCM	642:645	arg1	samples					647:653	SHCM samples	642:653	SHCM samples changed during 12 months, where in milk fat content was mostly affected by the season, followed by the lactose and protein contents	642:785	We also observed the contents of protein, fat, and lactose in SHCM samples changed during 12 months, where in milk fat content was mostly affected by the season, followed by the lactose and protein contents.
31132147	0	35	theme	Chemical	10:17	arg1	Composition					19:29	Chemical Composition	10:29	Chemical Composition of Simmental Crossbred Cattle Milk	10:64	Change in Chemical Composition of Simmental Crossbred Cattle Milk Improved its Physicochemical, Nutritional, and Processed Properties.
31132147	10	36	theme	different	1704:1712	arg1	products					1720:1727	different dairy products	1704:1727	different dairy products using raw milk from different seasons	1704:1765	All the data could be used to produce different dairy products using raw milk from different seasons.
31132147	4	37	theme	fat	695:697	arg1	content					699:705	milk fat content	690:705	milk fat content	690:705	We also observed the contents of protein, fat, and lactose in SHCM samples changed during 12 months, where in milk fat content was mostly affected by the season, followed by the lactose and protein contents.
31132147	3	38	theme	SHCM	428:431	arg1	samples					434:440	Simmental crossbred cattle milk (SHCM) samples	395:440	Simmental crossbred cattle milk (SHCM) samples	395:440	In addition, Simmental crossbred cattle milk (SHCM) samples had the higher contents of total amino acid than those of Chinese Holstein cow milk (HCM) samples, especially for Leu, Lys, Met, and Phe.
31132147	7	39	theme	crossbred	1138:1146	arg1	cattle					1148:1153	Simmental crossbred cattle	1128:1153	Simmental crossbred cattle	1128:1153	Logically, the milk from Simmental crossbred cattle has desirable physicochemical characteristics and nutritional value, which could be a new better resource of dairy products.
31132147	8	40	theme	disease	1435:1441	arg1	resistance					1443:1452	disease resistance	1435:1452	disease resistance	1435:1452	PRACTICAL APPLICATION: Compared with Chinese Holstein cattle, Simmental crossbred cattle have strong resistance to moist weather and better performance in disease resistance, which are particularly important for lactating cows persisting in south of China.
31132147	2	41	theme	raw	331:333	arg1	milk					335:338	raw milk	331:338	raw milk	331:338	In the experiment, physicochemical characterization of raw milk was evaluated according to the Standards.
31132147	8	42	from	resistance	1381:1390	arg1	resistance					1443:1452	disease resistance	1435:1452	disease resistance	1435:1452	PRACTICAL APPLICATION: Compared with Chinese Holstein cattle, Simmental crossbred cattle have strong resistance to moist weather and better performance in disease resistance, which are particularly important for lactating cows persisting in south of China.
31132147	9	43	from	variation	1616:1624	arg1	composition					1638:1648	chemical composition	1629:1648	chemical composition of SHCM sample	1629:1663	We evaluated the nutritional values of SHCM and HCM, and compared the seasonal variation on chemical composition of SHCM sample.
31132147	7	44	from	cattle	1148:1153	arg1	milk					1118:1121	the milk	1114:1121	the milk from Simmental crossbred cattle	1114:1153	Logically, the milk from Simmental crossbred cattle has desirable physicochemical characteristics and nutritional value, which could be a new better resource of dairy products.
31132147	6	45	theme	storage	977:983	arg1	time					985:988	storage time	977:988	storage time	977:988	The G' of yoghurt made from SHCM during storage time was always higher than that made from HCM, indicating that SHCM yoghurt showed higher apparent viscosity values.
31132147	10	46	theme	raw	1735:1737	arg1	milk					1739:1742	raw milk	1735:1742	raw milk from different seasons	1735:1765	All the data could be used to produce different dairy products using raw milk from different seasons.
31132147	1	47	dep	crossbred	199:207	arg1	Simmental					189:197	Simmental	189:197	Simmental	189:197	This study aimed to evaluate a new milk resource from Simmental crossbred cattle, which could be an alternative raw milk for dairy products.
31132147	7	48	dep	crossbred	1138:1146	arg1	Simmental					1128:1136	Simmental	1128:1136	Simmental	1128:1136	Logically, the milk from Simmental crossbred cattle has desirable physicochemical characteristics and nutritional value, which could be a new better resource of dairy products.
31132147	3	49	theme	acid	481:484	arg1	contents					457:464	the higher contents	446:464	the higher contents of total amino acid	446:484	In addition, Simmental crossbred cattle milk (SHCM) samples had the higher contents of total amino acid than those of Chinese Holstein cow milk (HCM) samples, especially for Leu, Lys, Met, and Phe.
31132147	8	50	theme	China	1530:1534	arg1	south					1521:1525	south	1521:1525	south	1521:1525	PRACTICAL APPLICATION: Compared with Chinese Holstein cattle, Simmental crossbred cattle have strong resistance to moist weather and better performance in disease resistance, which are particularly important for lactating cows persisting in south of China.
31132147	11	51	used	used	1890:1893	arg2	SHCM					1876:1879	SHCM	1876:1879	SHCM	1876:1879	In addition, textural properties of yoghurt made from two milks were also determined, and we suggested that SHCM could be used as a new resource candidate for developing dairy product in the future.
31132147	11	51	used	used	1890:1893	arg2	candidate					1913:1921	a new resource candidate	1898:1921	a new resource candidate for developing dairy product in the future	1898:1964	In addition, textural properties of yoghurt made from two milks were also determined, and we suggested that SHCM could be used as a new resource candidate for developing dairy product in the future.
31132147	4	52	dep	lactose	758:764	arg1	the					754:756	the	754:756	the	754:756	We also observed the contents of protein, fat, and lactose in SHCM samples changed during 12 months, where in milk fat content was mostly affected by the season, followed by the lactose and protein contents.
31132147	3	53	theme	total	469:473	arg1	acid					481:484	total amino acid	469:484	total amino acid	469:484	In addition, Simmental crossbred cattle milk (SHCM) samples had the higher contents of total amino acid than those of Chinese Holstein cow milk (HCM) samples, especially for Leu, Lys, Met, and Phe.
31132147	4	54	from	contents	601:608	arg1	samples					647:653	SHCM samples	642:653	SHCM samples changed during 12 months, where in milk fat content was mostly affected by the season, followed by the lactose and protein contents	642:785	We also observed the contents of protein, fat, and lactose in SHCM samples changed during 12 months, where in milk fat content was mostly affected by the season, followed by the lactose and protein contents.
31132147	5	55	contain	had	858:860	arg1	yoghurt					835:841	yoghurt	835:841	yoghurt made from SHCM	835:856	Moreover, texture profile analysis showed that yoghurt made from SHCM had stronger structures, which especially reflected in hardness and chewiness.
31132147	5	55	contain	had	858:860	arg2	structures					871:880	stronger structures	862:880	stronger structures	862:880	Moreover, texture profile analysis showed that yoghurt made from SHCM had stronger structures, which especially reflected in hardness and chewiness.
31132147	8	56	theme	lactating	1492:1500	arg1	cows					1502:1505	lactating cows	1492:1505	lactating cows persisting in south of China	1492:1534	PRACTICAL APPLICATION: Compared with Chinese Holstein cattle, Simmental crossbred cattle have strong resistance to moist weather and better performance in disease resistance, which are particularly important for lactating cows persisting in south of China.
31132147	3	57	theme	Chinese	500:506	arg1	samples					532:538	Chinese Holstein cow milk (HCM) samples	500:538	Chinese Holstein cow milk (HCM) samples	500:538	In addition, Simmental crossbred cattle milk (SHCM) samples had the higher contents of total amino acid than those of Chinese Holstein cow milk (HCM) samples, especially for Leu, Lys, Met, and Phe.
31132147	1	58	theme	raw	247:249	arg1	which					217:221	which	217:221	which	217:221	This study aimed to evaluate a new milk resource from Simmental crossbred cattle, which could be an alternative raw milk for dairy products.
31132147	1	58	theme	raw	247:249	arg1	milk					251:254	an alternative raw milk	232:254	an alternative raw milk for dairy products	232:273	This study aimed to evaluate a new milk resource from Simmental crossbred cattle, which could be an alternative raw milk for dairy products.
31132147	0	59	theme	Crossbred	44:52	arg1	Milk					61:64	Simmental Crossbred Cattle Milk	34:64	Simmental Crossbred Cattle Milk	34:64	Change in Chemical Composition of Simmental Crossbred Cattle Milk Improved its Physicochemical, Nutritional, and Processed Properties.
31132147	1	60	theme	milk	170:173	arg1	resource					175:182	a new milk resource	164:182	a new milk resource from Simmental crossbred cattle	164:214	This study aimed to evaluate a new milk resource from Simmental crossbred cattle, which could be an alternative raw milk for dairy products.
31132147	9	61	theme	nutritional	1554:1564	arg1	values					1566:1571	the nutritional values	1550:1571	the nutritional values of SHCM and HCM	1550:1587	We evaluated the nutritional values of SHCM and HCM, and compared the seasonal variation on chemical composition of SHCM sample.
31132147	7	62	theme	dairy	1264:1268	arg1	products					1270:1277	dairy products	1264:1277	dairy products	1264:1277	Logically, the milk from Simmental crossbred cattle has desirable physicochemical characteristics and nutritional value, which could be a new better resource of dairy products.
31132147	8	63	contain	have	1369:1372	arg2	performance					1420:1430	better performance	1413:1430	better performance in disease resistance, which are particularly important for lactating cows persisting in south of China	1413:1534	PRACTICAL APPLICATION: Compared with Chinese Holstein cattle, Simmental crossbred cattle have strong resistance to moist weather and better performance in disease resistance, which are particularly important for lactating cows persisting in south of China.
31132147	8	63	contain	have	1369:1372	arg2	resistance					1381:1390	strong resistance	1374:1390	strong resistance to moist weather	1374:1407	PRACTICAL APPLICATION: Compared with Chinese Holstein cattle, Simmental crossbred cattle have strong resistance to moist weather and better performance in disease resistance, which are particularly important for lactating cows persisting in south of China.
31132147	8	63	contain	have	1369:1372	arg1	Simmental					1342:1350	Simmental	1342:1350	Simmental	1342:1350	PRACTICAL APPLICATION: Compared with Chinese Holstein cattle, Simmental crossbred cattle have strong resistance to moist weather and better performance in disease resistance, which are particularly important for lactating cows persisting in south of China.
31132147	3	64	theme	cow	517:519	arg1	samples					532:538	Chinese Holstein cow milk (HCM) samples	500:538	Chinese Holstein cow milk (HCM) samples	500:538	In addition, Simmental crossbred cattle milk (SHCM) samples had the higher contents of total amino acid than those of Chinese Holstein cow milk (HCM) samples, especially for Leu, Lys, Met, and Phe.
31132147	10	65	from	seasons	1759:1765	arg1	milk					1739:1742	raw milk	1735:1742	raw milk from different seasons	1735:1765	All the data could be used to produce different dairy products using raw milk from different seasons.
31132147	11	66	theme	yoghurt	1804:1810	arg1	properties					1790:1799	textural properties	1781:1799	textural properties of yoghurt made from two milks	1781:1830	In addition, textural properties of yoghurt made from two milks were also determined, and we suggested that SHCM could be used as a new resource candidate for developing dairy product in the future.
31132147	1	67	theme	crossbred	199:207	arg1	cattle					209:214	Simmental crossbred cattle	189:214	Simmental crossbred cattle	189:214	This study aimed to evaluate a new milk resource from Simmental crossbred cattle, which could be an alternative raw milk for dairy products.
31132147	3	68	contain	had	442:444	arg1	samples					434:440	Simmental crossbred cattle milk (SHCM) samples	395:440	Simmental crossbred cattle milk (SHCM) samples	395:440	In addition, Simmental crossbred cattle milk (SHCM) samples had the higher contents of total amino acid than those of Chinese Holstein cow milk (HCM) samples, especially for Leu, Lys, Met, and Phe.
31132147	3	68	contain	had	442:444	arg2	contents					457:464	the higher contents	446:464	the higher contents of total amino acid	446:484	In addition, Simmental crossbred cattle milk (SHCM) samples had the higher contents of total amino acid than those of Chinese Holstein cow milk (HCM) samples, especially for Leu, Lys, Met, and Phe.
31132147	6	69	theme	higher	1069:1074	arg1	values					1095:1100	higher apparent viscosity values	1069:1100	higher apparent viscosity values	1069:1100	The G' of yoghurt made from SHCM during storage time was always higher than that made from HCM, indicating that SHCM yoghurt showed higher apparent viscosity values.
31132147	5	70	dep	had	858:860	arg1	reflected					900:908	reflected	900:908	reflected in hardness and chewiness	900:934	Moreover, texture profile analysis showed that yoghurt made from SHCM had stronger structures, which especially reflected in hardness and chewiness.
31132147	1	71	from	cattle	209:214	arg1	resource					175:182	a new milk resource	164:182	a new milk resource from Simmental crossbred cattle	164:214	This study aimed to evaluate a new milk resource from Simmental crossbred cattle, which could be an alternative raw milk for dairy products.
31132147	8	72	dep	APPLICATION	1290:1300	arg1	have					1369:1372	have	1369:1372	have strong resistance to moist weather and better performance in disease resistance, which are particularly important for lactating cows persisting in south of China	1369:1534	PRACTICAL APPLICATION: Compared with Chinese Holstein cattle, Simmental crossbred cattle have strong resistance to moist weather and better performance in disease resistance, which are particularly important for lactating cows persisting in south of China.
31132147	4	73	theme	protein	613:619	arg1	contents					601:608	the contents	597:608	the contents of protein, fat, and lactose in SHCM samples changed during 12 months, where in milk fat content was mostly affected by the season, followed by the lactose and protein contents	597:785	We also observed the contents of protein, fat, and lactose in SHCM samples changed during 12 months, where in milk fat content was mostly affected by the season, followed by the lactose and protein contents.
31132147	11	74	theme	new	1900:1902	arg1	SHCM					1876:1879	SHCM	1876:1879	SHCM	1876:1879	In addition, textural properties of yoghurt made from two milks were also determined, and we suggested that SHCM could be used as a new resource candidate for developing dairy product in the future.
31132147	11	74	theme	new	1900:1902	arg1	candidate					1913:1921	a new resource candidate	1898:1921	a new resource candidate for developing dairy product in the future	1898:1964	In addition, textural properties of yoghurt made from two milks were also determined, and we suggested that SHCM could be used as a new resource candidate for developing dairy product in the future.
31132147	4	75	theme	fat	622:624	arg1	contents					601:608	the contents	597:608	the contents of protein, fat, and lactose in SHCM samples changed during 12 months, where in milk fat content was mostly affected by the season, followed by the lactose and protein contents	597:785	We also observed the contents of protein, fat, and lactose in SHCM samples changed during 12 months, where in milk fat content was mostly affected by the season, followed by the lactose and protein contents.
31132147	9	76	theme	seasonal	1607:1614	arg1	variation					1616:1624	the seasonal variation	1603:1624	the seasonal variation on chemical composition of SHCM sample	1603:1663	We evaluated the nutritional values of SHCM and HCM, and compared the seasonal variation on chemical composition of SHCM sample.
31132147	0	77	theme	Physicochemical	79:93	arg1	Properties					123:132	its Physicochemical, Nutritional, and Processed Properties	75:132	its Physicochemical, Nutritional, and Processed Properties	75:132	Change in Chemical Composition of Simmental Crossbred Cattle Milk Improved its Physicochemical, Nutritional, and Processed Properties.
31132147	8	78	theme	strong	1374:1379	arg1	resistance					1381:1390	strong resistance	1374:1390	strong resistance to moist weather	1374:1407	PRACTICAL APPLICATION: Compared with Chinese Holstein cattle, Simmental crossbred cattle have strong resistance to moist weather and better performance in disease resistance, which are particularly important for lactating cows persisting in south of China.
31132147	0	79	theme	Nutritional	96:106	arg1	Properties					123:132	its Physicochemical, Nutritional, and Processed Properties	75:132	its Physicochemical, Nutritional, and Processed Properties	75:132	Change in Chemical Composition of Simmental Crossbred Cattle Milk Improved its Physicochemical, Nutritional, and Processed Properties.
31132147	2	80	theme	physicochemical	295:309	arg1	characterization					311:326	physicochemical characterization	295:326	physicochemical characterization of raw milk	295:338	In the experiment, physicochemical characterization of raw milk was evaluated according to the Standards.
31132147	9	81	theme	SHCM	1653:1656	arg1	sample					1658:1663	SHCM sample	1653:1663	SHCM sample	1653:1663	We evaluated the nutritional values of SHCM and HCM, and compared the seasonal variation on chemical composition of SHCM sample.
31132147	5	82	theme	texture	798:804	arg1	analysis					814:821	texture profile analysis	798:821	texture profile analysis	798:821	Moreover, texture profile analysis showed that yoghurt made from SHCM had stronger structures, which especially reflected in hardness and chewiness.
31132147	7	83	theme	desirable	1159:1167	arg1	characteristics					1185:1199	desirable physicochemical characteristics	1159:1199	desirable physicochemical characteristics	1159:1199	Logically, the milk from Simmental crossbred cattle has desirable physicochemical characteristics and nutritional value, which could be a new better resource of dairy products.
31132147	8	84	theme	better	1413:1418	arg1	performance					1420:1430	better performance	1413:1430	better performance in disease resistance, which are particularly important for lactating cows persisting in south of China	1413:1534	PRACTICAL APPLICATION: Compared with Chinese Holstein cattle, Simmental crossbred cattle have strong resistance to moist weather and better performance in disease resistance, which are particularly important for lactating cows persisting in south of China.
31132147	3	85	theme	cattle	415:420	arg1	samples					434:440	Simmental crossbred cattle milk (SHCM) samples	395:440	Simmental crossbred cattle milk (SHCM) samples	395:440	In addition, Simmental crossbred cattle milk (SHCM) samples had the higher contents of total amino acid than those of Chinese Holstein cow milk (HCM) samples, especially for Leu, Lys, Met, and Phe.
31132147	4	86	dep	affected	718:725	arg1	followed					742:749	followed	742:749	followed by the lactose and protein contents	742:785	We also observed the contents of protein, fat, and lactose in SHCM samples changed during 12 months, where in milk fat content was mostly affected by the season, followed by the lactose and protein contents.
31132147	1	87	theme	dairy	260:264	arg1	products					266:273	dairy products	260:273	dairy products	260:273	This study aimed to evaluate a new milk resource from Simmental crossbred cattle, which could be an alternative raw milk for dairy products.
31132147	10	88	theme	dairy	1714:1718	arg1	products					1720:1727	different dairy products	1704:1727	different dairy products using raw milk from different seasons	1704:1765	All the data could be used to produce different dairy products using raw milk from different seasons.
31132147	11	89	theme	textural	1781:1788	arg1	properties					1790:1799	textural properties	1781:1799	textural properties of yoghurt made from two milks	1781:1830	In addition, textural properties of yoghurt made from two milks were also determined, and we suggested that SHCM could be used as a new resource candidate for developing dairy product in the future.
31132147	0	90	theme	Simmental	34:42	arg1	Milk					61:64	Simmental Crossbred Cattle Milk	34:64	Simmental Crossbred Cattle Milk	34:64	Change in Chemical Composition of Simmental Crossbred Cattle Milk Improved its Physicochemical, Nutritional, and Processed Properties.
31132147	3	91	dep	crossbred	405:413	arg1	Simmental					395:403	Simmental	395:403	Simmental	395:403	In addition, Simmental crossbred cattle milk (SHCM) samples had the higher contents of total amino acid than those of Chinese Holstein cow milk (HCM) samples, especially for Leu, Lys, Met, and Phe.
31132147	4	92	theme	milk	690:693	arg1	fat					695:697	milk fat	690:697	milk fat content	690:705	We also observed the contents of protein, fat, and lactose in SHCM samples changed during 12 months, where in milk fat content was mostly affected by the season, followed by the lactose and protein contents.
31132147	0	93	theme	Cattle	54:59	arg1	Milk					61:64	Simmental Crossbred Cattle Milk	34:64	Simmental Crossbred Cattle Milk	34:64	Change in Chemical Composition of Simmental Crossbred Cattle Milk Improved its Physicochemical, Nutritional, and Processed Properties.
31132147	3	94	theme	amino	475:479	arg1	acid					481:484	total amino acid	469:484	total amino acid	469:484	In addition, Simmental crossbred cattle milk (SHCM) samples had the higher contents of total amino acid than those of Chinese Holstein cow milk (HCM) samples, especially for Leu, Lys, Met, and Phe.
31132147	8	95	theme	Chinese	1317:1323	arg1	cattle					1334:1339	Chinese Holstein cattle	1317:1339	Chinese Holstein cattle	1317:1339	PRACTICAL APPLICATION: Compared with Chinese Holstein cattle, Simmental crossbred cattle have strong resistance to moist weather and better performance in disease resistance, which are particularly important for lactating cows persisting in south of China.
31132147	7	96	theme	new	1241:1243	arg1	resource					1252:1259	a new better resource	1239:1259	a new better resource of dairy products	1239:1277	Logically, the milk from Simmental crossbred cattle has desirable physicochemical characteristics and nutritional value, which could be a new better resource of dairy products.
31132147	7	96	theme	new	1241:1243	arg1	value					1217:1221	nutritional value	1205:1221	nutritional value	1205:1221	Logically, the milk from Simmental crossbred cattle has desirable physicochemical characteristics and nutritional value, which could be a new better resource of dairy products.
31132147	3	97	theme	higher	450:455	arg1	contents					457:464	the higher contents	446:464	the higher contents of total amino acid	446:484	In addition, Simmental crossbred cattle milk (SHCM) samples had the higher contents of total amino acid than those of Chinese Holstein cow milk (HCM) samples, especially for Leu, Lys, Met, and Phe.
31132147	5	98	theme	stronger	862:869	arg1	structures					871:880	stronger structures	862:880	stronger structures	862:880	Moreover, texture profile analysis showed that yoghurt made from SHCM had stronger structures, which especially reflected in hardness and chewiness.
31132147	8	99	theme	PRACTICAL	1280:1288	arg1	APPLICATION					1290:1300	PRACTICAL APPLICATION	1280:1300	PRACTICAL APPLICATION: Compared with Chinese Holstein cattle, Simmental crossbred cattle have strong resistance to moist weather and better performance in disease resistance, which are particularly important for lactating cows persisting in south of China.	1280:1535	PRACTICAL APPLICATION: Compared with Chinese Holstein cattle, Simmental crossbred cattle have strong resistance to moist weather and better performance in disease resistance, which are particularly important for lactating cows persisting in south of China.
29632975	8	0	theme	Agromyces	1061:1069	arg1	sp					1080:1081	the name Agromyces mangrovi sp	1052:1081	the name Agromyces mangrovi sp	1052:1081	The phylogenetic analysis and DNA-DNA hybridization, along with the phenotypic and chemotaxonomic characteristics, indicate that strain Q14T as a novel species of the genus Agromyces, for which the name Agromyces mangrovi sp.
29632975	6	1	theme	major	633:637	arg1	lipids					645:650	The major polar lipids	629:650	The major polar lipids of strain Q14T	629:665	The major polar lipids of strain Q14T were diphosphatidylglycerol, phosphatidylglycerol and one glycolipid.
29632975	6	1	theme	major	633:637	arg1	diphosphatidylglycerol					672:693	diphosphatidylglycerol	672:693	diphosphatidylglycerol	672:693	The major polar lipids of strain Q14T were diphosphatidylglycerol, phosphatidylglycerol and one glycolipid.
29632975	7	2	theme	glutamic	809:816	arg1	acid					782:785	2,4-diaminobutylic acid	763:785	2,4-diaminobutylic acid (A2bu)	763:792	The strain Q14T contained 2,4-diaminobutylic acid (A2bu), alanine acid, glutamic acid and glycine in the peptidoglycans.
29632975	7	2	theme	glutamic	809:816	arg1	acid					818:821	glutamic acid	809:821	glutamic acid	809:821	The strain Q14T contained 2,4-diaminobutylic acid (A2bu), alanine acid, glutamic acid and glycine in the peptidoglycans.
29632975	8	3	theme	name	1056:1059	arg1	sp					1080:1081	the name Agromyces mangrovi sp	1052:1081	the name Agromyces mangrovi sp	1052:1081	The phylogenetic analysis and DNA-DNA hybridization, along with the phenotypic and chemotaxonomic characteristics, indicate that strain Q14T as a novel species of the genus Agromyces, for which the name Agromyces mangrovi sp.
29632975	2	4	theme	Gram-stain-positive	91:109	arg1	bacterium					188:196	A novel, Gram-stain-positive, microaerophilic to aerobic, non-endospore-forming, no-motile and rod-shaped bacterium	82:196	A novel, Gram-stain-positive, microaerophilic to aerobic, non-endospore-forming, no-motile and rod-shaped bacterium designated Q14T	82:212	A novel, Gram-stain-positive, microaerophilic to aerobic, non-endospore-forming, no-motile and rod-shaped bacterium designated Q14T was isolated from mangrove soil samples collected on chengmai, Hainan province, China.
29632975	5	5	theme	major	562:566	arg1	anteiso-C15:0					585:597	anteiso-C15:0	585:597	anteiso-C15:0	585:597	The chemotaxonomic analysis showed that the predominant isoprenoid quinone was MK-12 and the major fatty acids were anteiso-C15:0, iso-C17:0 and anteiso-C17:0.
29632975	5	5	theme	major	562:566	arg1	acids					574:578	the major fatty acids	558:578	the major fatty acids	558:578	The chemotaxonomic analysis showed that the predominant isoprenoid quinone was MK-12 and the major fatty acids were anteiso-C15:0, iso-C17:0 and anteiso-C17:0.
29632975	6	6	dep	diphosphatidylglycerol	672:693	arg1	glycolipid					725:734	glycolipid	725:734	glycolipid	725:734	The major polar lipids of strain Q14T were diphosphatidylglycerol, phosphatidylglycerol and one glycolipid.
29632975	5	7	theme	fatty	568:572	arg1	anteiso-C15:0					585:597	anteiso-C15:0	585:597	anteiso-C15:0	585:597	The chemotaxonomic analysis showed that the predominant isoprenoid quinone was MK-12 and the major fatty acids were anteiso-C15:0, iso-C17:0 and anteiso-C17:0.
29632975	5	7	theme	fatty	568:572	arg1	acids					574:578	the major fatty acids	558:578	the major fatty acids	558:578	The chemotaxonomic analysis showed that the predominant isoprenoid quinone was MK-12 and the major fatty acids were anteiso-C15:0, iso-C17:0 and anteiso-C17:0.
29632975	2	8	attach	isolated	218:225	arg2	bacterium					188:196	A novel, Gram-stain-positive, microaerophilic to aerobic, non-endospore-forming, no-motile and rod-shaped bacterium	82:196	A novel, Gram-stain-positive, microaerophilic to aerobic, non-endospore-forming, no-motile and rod-shaped bacterium designated Q14T	82:212	A novel, Gram-stain-positive, microaerophilic to aerobic, non-endospore-forming, no-motile and rod-shaped bacterium designated Q14T was isolated from mangrove soil samples collected on chengmai, Hainan province, China.
29632975	2	8	attach	isolated	218:225	arg1	samples					246:252	mangrove soil samples	232:252	mangrove soil samples collected on chengmai, Hainan province, China	232:298	A novel, Gram-stain-positive, microaerophilic to aerobic, non-endospore-forming, no-motile and rod-shaped bacterium designated Q14T was isolated from mangrove soil samples collected on chengmai, Hainan province, China.
29632975	3	9	theme	optimum	343:349	arg1	10-40 °C					333:340	10-40 °C	333:340	10-40 °C (optimum 30 °C)	333:356	Strain Q14T was able to grow at 10-40 °C (optimum 30 °C), pH 5.5-10.0 (optimum 6.5-8.0) and with 0.5-6% (w/v) NaCl (optimum 1%).
29632975	3	9	theme	optimum	343:349	arg1	30 °C					351:355	optimum 30 °C	343:355	optimum 30 °C	343:355	Strain Q14T was able to grow at 10-40 °C (optimum 30 °C), pH 5.5-10.0 (optimum 6.5-8.0) and with 0.5-6% (w/v) NaCl (optimum 1%).
29632975	2	10	theme	non-endospore-forming	140:160	arg1	bacterium					188:196	A novel, Gram-stain-positive, microaerophilic to aerobic, non-endospore-forming, no-motile and rod-shaped bacterium	82:196	A novel, Gram-stain-positive, microaerophilic to aerobic, non-endospore-forming, no-motile and rod-shaped bacterium designated Q14T	82:212	A novel, Gram-stain-positive, microaerophilic to aerobic, non-endospore-forming, no-motile and rod-shaped bacterium designated Q14T was isolated from mangrove soil samples collected on chengmai, Hainan province, China.
29632975	5	11	theme	predominant	513:523	arg1	MK-12					548:552	MK-12	548:552	MK-12	548:552	The chemotaxonomic analysis showed that the predominant isoprenoid quinone was MK-12 and the major fatty acids were anteiso-C15:0, iso-C17:0 and anteiso-C17:0.
29632975	5	11	theme	predominant	513:523	arg1	quinone					536:542	the predominant isoprenoid quinone	509:542	the predominant isoprenoid quinone	509:542	The chemotaxonomic analysis showed that the predominant isoprenoid quinone was MK-12 and the major fatty acids were anteiso-C15:0, iso-C17:0 and anteiso-C17:0.
29632975	10	12	theme	type	1106:1109	arg1	strain					1111:1116	The type strain	1102:1116	The type strain	1102:1116	The type strain is Q14T (= MCCC 1K03191T = KCTC 39814T).
29632975	10	12	theme	type	1106:1109	arg1	Q14T					1121:1124	Q14T	1121:1124	Q14T (= MCCC 1K03191T = KCTC 39814T)	1121:1156	The type strain is Q14T (= MCCC 1K03191T = KCTC 39814T).
29632975	5	13	theme	isoprenoid	525:534	arg1	MK-12					548:552	MK-12	548:552	MK-12	548:552	The chemotaxonomic analysis showed that the predominant isoprenoid quinone was MK-12 and the major fatty acids were anteiso-C15:0, iso-C17:0 and anteiso-C17:0.
29632975	5	13	theme	isoprenoid	525:534	arg1	quinone					536:542	the predominant isoprenoid quinone	509:542	the predominant isoprenoid quinone	509:542	The chemotaxonomic analysis showed that the predominant isoprenoid quinone was MK-12 and the major fatty acids were anteiso-C15:0, iso-C17:0 and anteiso-C17:0.
29632975	2	14	theme	aerobic	131:137	arg1	bacterium					188:196	A novel, Gram-stain-positive, microaerophilic to aerobic, non-endospore-forming, no-motile and rod-shaped bacterium	82:196	A novel, Gram-stain-positive, microaerophilic to aerobic, non-endospore-forming, no-motile and rod-shaped bacterium designated Q14T	82:212	A novel, Gram-stain-positive, microaerophilic to aerobic, non-endospore-forming, no-motile and rod-shaped bacterium designated Q14T was isolated from mangrove soil samples collected on chengmai, Hainan province, China.
29632975	8	15	theme	strain	987:992	arg1	Q14T					994:997	that strain Q14T	982:997	that strain Q14T	982:997	The phylogenetic analysis and DNA-DNA hybridization, along with the phenotypic and chemotaxonomic characteristics, indicate that strain Q14T as a novel species of the genus Agromyces, for which the name Agromyces mangrovi sp.
29632975	8	15	theme	strain	987:992	arg1	species					1010:1016	a novel species	1002:1016	a novel species	1002:1016	The phylogenetic analysis and DNA-DNA hybridization, along with the phenotypic and chemotaxonomic characteristics, indicate that strain Q14T as a novel species of the genus Agromyces, for which the name Agromyces mangrovi sp.
29632975	8	16	theme	phenotypic	926:935	arg1	characteristics					956:970	the phenotypic and chemotaxonomic characteristics	922:970	the phenotypic and chemotaxonomic characteristics	922:970	The phylogenetic analysis and DNA-DNA hybridization, along with the phenotypic and chemotaxonomic characteristics, indicate that strain Q14T as a novel species of the genus Agromyces, for which the name Agromyces mangrovi sp.
29632975	1	17	theme	Mangrove	67:74	arg1	Soil					76:79	Mangrove Soil	67:79	Mangrove Soil	67:79	nov., a Novel Actinobacterium Isolated from Mangrove Soil.
29632975	0	18	theme	mangrovi	10:17	arg1	sp					19:20	mangrovi sp	10:20	mangrovi sp	10:20	Agromyces mangrovi sp.
29632975	5	19	theme	chemotaxonomic	473:486	arg1	analysis					488:495	The chemotaxonomic analysis	469:495	The chemotaxonomic analysis	469:495	The chemotaxonomic analysis showed that the predominant isoprenoid quinone was MK-12 and the major fatty acids were anteiso-C15:0, iso-C17:0 and anteiso-C17:0.
29632975	7	20	from	acid	818:821	arg1	peptidoglycans					842:855	the peptidoglycans	838:855	the peptidoglycans	838:855	The strain Q14T contained 2,4-diaminobutylic acid (A2bu), alanine acid, glutamic acid and glycine in the peptidoglycans.
29632975	2	21	dep	aerobic	131:137	arg1	to					128:129	to	128:129	to	128:129	A novel, Gram-stain-positive, microaerophilic to aerobic, non-endospore-forming, no-motile and rod-shaped bacterium designated Q14T was isolated from mangrove soil samples collected on chengmai, Hainan province, China.
29632975	8	22	theme	chemotaxonomic	941:954	arg1	characteristics					956:970	the phenotypic and chemotaxonomic characteristics	922:970	the phenotypic and chemotaxonomic characteristics	922:970	The phylogenetic analysis and DNA-DNA hybridization, along with the phenotypic and chemotaxonomic characteristics, indicate that strain Q14T as a novel species of the genus Agromyces, for which the name Agromyces mangrovi sp.
29632975	7	23	theme	2,4-diaminobutylic	763:780	arg1	A2bu					788:791	A2bu	788:791	A2bu	788:791	The strain Q14T contained 2,4-diaminobutylic acid (A2bu), alanine acid, glutamic acid and glycine in the peptidoglycans.
29632975	7	23	theme	2,4-diaminobutylic	763:780	arg1	acid					782:785	2,4-diaminobutylic acid	763:785	2,4-diaminobutylic acid (A2bu)	763:792	The strain Q14T contained 2,4-diaminobutylic acid (A2bu), alanine acid, glutamic acid and glycine in the peptidoglycans.
29632975	7	23	theme	2,4-diaminobutylic	763:780	arg1	glycine					827:833	glycine	827:833	glycine	827:833	The strain Q14T contained 2,4-diaminobutylic acid (A2bu), alanine acid, glutamic acid and glycine in the peptidoglycans.
29632975	7	23	theme	2,4-diaminobutylic	763:780	arg1	acid					803:806	alanine acid	795:806	alanine acid	795:806	The strain Q14T contained 2,4-diaminobutylic acid (A2bu), alanine acid, glutamic acid and glycine in the peptidoglycans.
29632975	7	23	theme	2,4-diaminobutylic	763:780	arg1	acid					818:821	glutamic acid	809:821	glutamic acid	809:821	The strain Q14T contained 2,4-diaminobutylic acid (A2bu), alanine acid, glutamic acid and glycine in the peptidoglycans.
29632975	7	24	theme	strain	741:746	arg1	Q14T					748:751	The strain Q14T	737:751	The strain Q14T	737:751	The strain Q14T contained 2,4-diaminobutylic acid (A2bu), alanine acid, glutamic acid and glycine in the peptidoglycans.
29632975	7	25	contain	contained	753:761	arg1	Q14T					748:751	The strain Q14T	737:751	The strain Q14T	737:751	The strain Q14T contained 2,4-diaminobutylic acid (A2bu), alanine acid, glutamic acid and glycine in the peptidoglycans.
29632975	7	25	contain	contained	753:761	arg2	A2bu					788:791	A2bu	788:791	A2bu	788:791	The strain Q14T contained 2,4-diaminobutylic acid (A2bu), alanine acid, glutamic acid and glycine in the peptidoglycans.
29632975	7	25	contain	contained	753:761	arg2	acid					818:821	glutamic acid	809:821	glutamic acid	809:821	The strain Q14T contained 2,4-diaminobutylic acid (A2bu), alanine acid, glutamic acid and glycine in the peptidoglycans.
29632975	7	25	contain	contained	753:761	arg2	glycine					827:833	glycine	827:833	glycine	827:833	The strain Q14T contained 2,4-diaminobutylic acid (A2bu), alanine acid, glutamic acid and glycine in the peptidoglycans.
29632975	7	25	contain	contained	753:761	arg2	acid					782:785	2,4-diaminobutylic acid	763:785	2,4-diaminobutylic acid (A2bu)	763:792	The strain Q14T contained 2,4-diaminobutylic acid (A2bu), alanine acid, glutamic acid and glycine in the peptidoglycans.
29632975	7	25	contain	contained	753:761	arg2	acid					803:806	alanine acid	795:806	alanine acid	795:806	The strain Q14T contained 2,4-diaminobutylic acid (A2bu), alanine acid, glutamic acid and glycine in the peptidoglycans.
29632975	7	26	from	glycine	827:833	arg1	peptidoglycans					842:855	the peptidoglycans	838:855	the peptidoglycans	838:855	The strain Q14T contained 2,4-diaminobutylic acid (A2bu), alanine acid, glutamic acid and glycine in the peptidoglycans.
29632975	3	27	theme	w/v	406:408	arg1	NaCl					411:414	0.5-6% (w/v) NaCl	398:414	0.5-6% (w/v) NaCl (optimum 1%)	398:427	Strain Q14T was able to grow at 10-40 °C (optimum 30 °C), pH 5.5-10.0 (optimum 6.5-8.0) and with 0.5-6% (w/v) NaCl (optimum 1%).
29632975	3	27	theme	w/v	406:408	arg1	%					426:426	optimum 1%	417:426	optimum 1%	417:426	Strain Q14T was able to grow at 10-40 °C (optimum 30 °C), pH 5.5-10.0 (optimum 6.5-8.0) and with 0.5-6% (w/v) NaCl (optimum 1%).
29632975	8	28	theme	novel	1004:1008	arg1	Q14T					994:997	that strain Q14T	982:997	that strain Q14T	982:997	The phylogenetic analysis and DNA-DNA hybridization, along with the phenotypic and chemotaxonomic characteristics, indicate that strain Q14T as a novel species of the genus Agromyces, for which the name Agromyces mangrovi sp.
29632975	8	28	theme	novel	1004:1008	arg1	species					1010:1016	a novel species	1002:1016	a novel species	1002:1016	The phylogenetic analysis and DNA-DNA hybridization, along with the phenotypic and chemotaxonomic characteristics, indicate that strain Q14T as a novel species of the genus Agromyces, for which the name Agromyces mangrovi sp.
29632975	2	29	theme	rod-shaped	177:186	arg1	bacterium					188:196	A novel, Gram-stain-positive, microaerophilic to aerobic, non-endospore-forming, no-motile and rod-shaped bacterium	82:196	A novel, Gram-stain-positive, microaerophilic to aerobic, non-endospore-forming, no-motile and rod-shaped bacterium designated Q14T	82:212	A novel, Gram-stain-positive, microaerophilic to aerobic, non-endospore-forming, no-motile and rod-shaped bacterium designated Q14T was isolated from mangrove soil samples collected on chengmai, Hainan province, China.
29632975	8	30	theme	Agromyces	1031:1039	arg1	Q14T					994:997	that strain Q14T	982:997	that strain Q14T	982:997	The phylogenetic analysis and DNA-DNA hybridization, along with the phenotypic and chemotaxonomic characteristics, indicate that strain Q14T as a novel species of the genus Agromyces, for which the name Agromyces mangrovi sp.
29632975	8	30	theme	Agromyces	1031:1039	arg1	species					1010:1016	a novel species	1002:1016	a novel species	1002:1016	The phylogenetic analysis and DNA-DNA hybridization, along with the phenotypic and chemotaxonomic characteristics, indicate that strain Q14T as a novel species of the genus Agromyces, for which the name Agromyces mangrovi sp.
29632975	7	31	theme	alanine	795:801	arg1	acid					782:785	2,4-diaminobutylic acid	763:785	2,4-diaminobutylic acid (A2bu)	763:792	The strain Q14T contained 2,4-diaminobutylic acid (A2bu), alanine acid, glutamic acid and glycine in the peptidoglycans.
29632975	7	31	theme	alanine	795:801	arg1	acid					803:806	alanine acid	795:806	alanine acid	795:806	The strain Q14T contained 2,4-diaminobutylic acid (A2bu), alanine acid, glutamic acid and glycine in the peptidoglycans.
29632975	2	32	theme	soil	241:244	arg1	samples					246:252	mangrove soil samples	232:252	mangrove soil samples collected on chengmai, Hainan province, China	232:298	A novel, Gram-stain-positive, microaerophilic to aerobic, non-endospore-forming, no-motile and rod-shaped bacterium designated Q14T was isolated from mangrove soil samples collected on chengmai, Hainan province, China.
29632975	3	33	theme	%	403:403	arg1	NaCl					411:414	0.5-6% (w/v) NaCl	398:414	0.5-6% (w/v) NaCl (optimum 1%)	398:427	Strain Q14T was able to grow at 10-40 °C (optimum 30 °C), pH 5.5-10.0 (optimum 6.5-8.0) and with 0.5-6% (w/v) NaCl (optimum 1%).
29632975	3	33	theme	%	403:403	arg1	%					426:426	optimum 1%	417:426	optimum 1%	417:426	Strain Q14T was able to grow at 10-40 °C (optimum 30 °C), pH 5.5-10.0 (optimum 6.5-8.0) and with 0.5-6% (w/v) NaCl (optimum 1%).
29632975	2	34	theme	novel	84:88	arg1	bacterium					188:196	A novel, Gram-stain-positive, microaerophilic to aerobic, non-endospore-forming, no-motile and rod-shaped bacterium	82:196	A novel, Gram-stain-positive, microaerophilic to aerobic, non-endospore-forming, no-motile and rod-shaped bacterium designated Q14T	82:212	A novel, Gram-stain-positive, microaerophilic to aerobic, non-endospore-forming, no-motile and rod-shaped bacterium designated Q14T was isolated from mangrove soil samples collected on chengmai, Hainan province, China.
29632975	2	35	theme	no-motile	163:171	arg1	bacterium					188:196	A novel, Gram-stain-positive, microaerophilic to aerobic, non-endospore-forming, no-motile and rod-shaped bacterium	82:196	A novel, Gram-stain-positive, microaerophilic to aerobic, non-endospore-forming, no-motile and rod-shaped bacterium designated Q14T	82:212	A novel, Gram-stain-positive, microaerophilic to aerobic, non-endospore-forming, no-motile and rod-shaped bacterium designated Q14T was isolated from mangrove soil samples collected on chengmai, Hainan province, China.
29632975	2	36	theme	mangrove	232:239	arg1	samples					246:252	mangrove soil samples	232:252	mangrove soil samples collected on chengmai, Hainan province, China	232:298	A novel, Gram-stain-positive, microaerophilic to aerobic, non-endospore-forming, no-motile and rod-shaped bacterium designated Q14T was isolated from mangrove soil samples collected on chengmai, Hainan province, China.
29632975	4	37	theme	DNA	442:444	arg1	content					450:456	The genomic DNA G+C content	430:456	The genomic DNA G+C content	430:456	The genomic DNA G+C content was 70.1%.
29632975	4	37	theme	DNA	442:444	arg1	%					466:466	70.1%	462:466	70.1%	462:466	The genomic DNA G+C content was 70.1%.
29632975	3	38	theme	Strain	301:306	arg1	Q14T					308:311	Strain Q14T	301:311	Strain Q14T	301:311	Strain Q14T was able to grow at 10-40 °C (optimum 30 °C), pH 5.5-10.0 (optimum 6.5-8.0) and with 0.5-6% (w/v) NaCl (optimum 1%).
29632975	3	39	theme	optimum	417:423	arg1	NaCl					411:414	0.5-6% (w/v) NaCl	398:414	0.5-6% (w/v) NaCl (optimum 1%)	398:427	Strain Q14T was able to grow at 10-40 °C (optimum 30 °C), pH 5.5-10.0 (optimum 6.5-8.0) and with 0.5-6% (w/v) NaCl (optimum 1%).
29632975	3	39	theme	optimum	417:423	arg1	%					426:426	optimum 1%	417:426	optimum 1%	417:426	Strain Q14T was able to grow at 10-40 °C (optimum 30 °C), pH 5.5-10.0 (optimum 6.5-8.0) and with 0.5-6% (w/v) NaCl (optimum 1%).
29632975	8	40	theme	genus	1025:1029	arg1	Agromyces					1031:1039	the genus Agromyces	1021:1039	the genus Agromyces	1021:1039	The phylogenetic analysis and DNA-DNA hybridization, along with the phenotypic and chemotaxonomic characteristics, indicate that strain Q14T as a novel species of the genus Agromyces, for which the name Agromyces mangrovi sp.
29632975	6	41	theme	Q14T	662:665	arg1	lipids					645:650	The major polar lipids	629:650	The major polar lipids of strain Q14T	629:665	The major polar lipids of strain Q14T were diphosphatidylglycerol, phosphatidylglycerol and one glycolipid.
29632975	6	41	theme	Q14T	662:665	arg1	diphosphatidylglycerol					672:693	diphosphatidylglycerol	672:693	diphosphatidylglycerol	672:693	The major polar lipids of strain Q14T were diphosphatidylglycerol, phosphatidylglycerol and one glycolipid.
29632975	4	42	theme	G+C	446:448	arg1	content					450:456	The genomic DNA G+C content	430:456	The genomic DNA G+C content	430:456	The genomic DNA G+C content was 70.1%.
29632975	4	42	theme	G+C	446:448	arg1	%					466:466	70.1%	462:466	70.1%	462:466	The genomic DNA G+C content was 70.1%.
29632975	6	43	theme	strain	655:660	arg1	Q14T					662:665	strain Q14T	655:665	strain Q14T	655:665	The major polar lipids of strain Q14T were diphosphatidylglycerol, phosphatidylglycerol and one glycolipid.
29632975	8	44	theme	DNA-DNA	888:894	arg1	hybridization					896:908	DNA-DNA hybridization	888:908	DNA-DNA hybridization	888:908	The phylogenetic analysis and DNA-DNA hybridization, along with the phenotypic and chemotaxonomic characteristics, indicate that strain Q14T as a novel species of the genus Agromyces, for which the name Agromyces mangrovi sp.
29632975	0	45	dep	sp	19:20	arg1	Agromyces					0:8	Agromyces	0:8	Agromyces	0:8	Agromyces mangrovi sp.
29632975	10	46	theme	= MCCC	1127:1132	arg1	39814T					1150:1155	= MCCC 1K03191T = KCTC 39814T	1127:1155	= MCCC 1K03191T = KCTC 39814T	1127:1155	The type strain is Q14T (= MCCC 1K03191T = KCTC 39814T).
29632975	10	46	theme	= MCCC	1127:1132	arg1	Q14T					1121:1124	Q14T	1121:1124	Q14T (= MCCC 1K03191T = KCTC 39814T)	1121:1156	The type strain is Q14T (= MCCC 1K03191T = KCTC 39814T).
29632975	8	47	theme	phylogenetic	862:873	arg1	analysis					875:882	The phylogenetic analysis	858:882	The phylogenetic analysis	858:882	The phylogenetic analysis and DNA-DNA hybridization, along with the phenotypic and chemotaxonomic characteristics, indicate that strain Q14T as a novel species of the genus Agromyces, for which the name Agromyces mangrovi sp.
29632975	10	48	theme	1K03191T = KCTC	1134:1148	arg1	39814T					1150:1155	= MCCC 1K03191T = KCTC 39814T	1127:1155	= MCCC 1K03191T = KCTC 39814T	1127:1155	The type strain is Q14T (= MCCC 1K03191T = KCTC 39814T).
29632975	10	48	theme	1K03191T = KCTC	1134:1148	arg1	Q14T					1121:1124	Q14T	1121:1124	Q14T (= MCCC 1K03191T = KCTC 39814T)	1121:1156	The type strain is Q14T (= MCCC 1K03191T = KCTC 39814T).
29632975	1	49	theme	Novel	31:35	arg1	Actinobacterium					37:51	a Novel Actinobacterium	29:51	a Novel Actinobacterium	29:51	nov., a Novel Actinobacterium Isolated from Mangrove Soil.
29632975	1	49	theme	Novel	31:35	arg1	nov.					23:26	nov.	23:26	nov.	23:26	nov., a Novel Actinobacterium Isolated from Mangrove Soil.
29632975	6	50	theme	polar	639:643	arg1	lipids					645:650	The major polar lipids	629:650	The major polar lipids of strain Q14T	629:665	The major polar lipids of strain Q14T were diphosphatidylglycerol, phosphatidylglycerol and one glycolipid.
29632975	6	50	theme	polar	639:643	arg1	diphosphatidylglycerol					672:693	diphosphatidylglycerol	672:693	diphosphatidylglycerol	672:693	The major polar lipids of strain Q14T were diphosphatidylglycerol, phosphatidylglycerol and one glycolipid.
29632975	4	51	theme	genomic	434:440	arg1	content					450:456	The genomic DNA G+C content	430:456	The genomic DNA G+C content	430:456	The genomic DNA G+C content was 70.1%.
29632975	4	51	theme	genomic	434:440	arg1	%					466:466	70.1%	462:466	70.1%	462:466	The genomic DNA G+C content was 70.1%.
31534334	7	0	dep	In	1136:1137	arg1	vivo					1139:1142	vivo	1139:1142	vivo	1139:1142	In vivo animal models of critical bone defects in the radius of rabbit were used.
31534334	3	1	theme	composite	556:564	arg1	scaffold					566:573	An icariin-loaded hydroxyapatite/alginate porous composite scaffold	507:573	An icariin-loaded hydroxyapatite/alginate porous composite scaffold	507:573	METHODS An icariin-loaded hydroxyapatite/alginate porous composite scaffold was prepared and characterized by SEM and HPLC for morphology and release behavior, respectively.
31534334	8	2	theme	scoring	1393:1399	arg1	system					1401:1406	the Lane and Sandhu X-ray scoring system	1367:1406	the Lane and Sandhu X-ray scoring system	1367:1406	Four and 12 weeks after the implantation of IC/HAA scaffolds in the bone defect, radiographic images of the radius were obtained and scored by using the Lane and Sandhu X-ray scoring system.
31534334	7	3	theme	bone	1170:1173	arg1	defects					1175:1181	critical bone defects	1161:1181	critical bone defects in the radius of rabbit	1161:1205	In vivo animal models of critical bone defects in the radius of rabbit were used.
31534334	13	4	theme	bone	2040:2043	arg1	regeneration					2045:2056	bone regeneration	2040:2056	bone regeneration	2040:2056	In vivo, rabbit radius bone defect experiments demonstrated that the IC/HAA scaffold exhibited better capacity for bone regeneration than HAA, and IC/HAA upregulated the relative expression levels of an osteogenic gene and the Wnt signaling pathway genes.
31534334	4	5	from	cytotoxicity	723:734	arg1	PBS					715:717	PBS	715:717	PBS	715:717	The mechanical properties, degradation in PBS and cytotoxicity on BMSCs were also evaluated by MTT assay, compression strength and calculation of weight remaining ratio, respectively.
31534334	4	5	from	cytotoxicity	723:734	arg1	BMSCs					739:743	BMSCs	739:743	BMSCs	739:743	The mechanical properties, degradation in PBS and cytotoxicity on BMSCs were also evaluated by MTT assay, compression strength and calculation of weight remaining ratio, respectively.
31534334	13	6	theme	IC/HAA	1994:1999	arg1	scaffold					2001:2008	the IC/HAA scaffold	1990:2008	the IC/HAA scaffold	1990:2008	In vivo, rabbit radius bone defect experiments demonstrated that the IC/HAA scaffold exhibited better capacity for bone regeneration than HAA, and IC/HAA upregulated the relative expression levels of an osteogenic gene and the Wnt signaling pathway genes.
31534334	1	7	theme	osteogenic	176:185	arg1	differentiation					187:201	osteogenic differentiation	176:201	osteogenic differentiation	176:201	OBJECTIVE Icariin (IC) promotes osteogenic differentiation, and it may be a potential small molecule drug for local application in bone regeneration.
31534334	8	8	from	implantation	1246:1257	arg1	defect					1291:1296	the bone defect	1282:1296	the bone defect	1282:1296	Four and 12 weeks after the implantation of IC/HAA scaffolds in the bone defect, radiographic images of the radius were obtained and scored by using the Lane and Sandhu X-ray scoring system.
31534334	7	9	theme	animal	1144:1149	arg1	models					1151:1156	In vivo animal models	1136:1156	In vivo animal models of critical bone defects in the radius of rabbit	1136:1205	In vivo animal models of critical bone defects in the radius of rabbit were used.
31534334	4	10	from	degradation	700:710	arg1	PBS					715:717	PBS	715:717	PBS	715:717	The mechanical properties, degradation in PBS and cytotoxicity on BMSCs were also evaluated by MTT assay, compression strength and calculation of weight remaining ratio, respectively.
31534334	4	10	from	degradation	700:710	arg1	BMSCs					739:743	BMSCs	739:743	BMSCs	739:743	The mechanical properties, degradation in PBS and cytotoxicity on BMSCs were also evaluated by MTT assay, compression strength and calculation of weight remaining ratio, respectively.
31534334	6	11	theme	protein	1030:1036	arg1	level					1049:1053	The mRNA and protein expression level	1017:1053	The mRNA and protein expression level of an osteogenic gene	1017:1075	The mRNA and protein expression level of an osteogenic gene was detected by RT-PCR and Western blotting, respectively.
31534334	9	12	theme	Masson	1458:1463	arg1	staining					1465:1472	H&E and Masson staining	1450:1472	staining	1465:1472	Tissue samples were also evaluated using H&E and Masson staining, and an osteogenic gene and Wnt signaling pathway genes were detected.
31534334	15	13	dep	CONCLUSION	2259:2268	arg1	suggests					2279:2286	suggests	2279:2286	suggests a promising application for the use of HAA scaffolds to load icariin	2279:2355	CONCLUSION Our data suggests a promising application for the use of HAA scaffolds to load icariin and promote bone regeneration in situ through mediation of the coupling processes of osteogenesis induction and osteoclast activity inhibition.
31534334	15	13	dep	CONCLUSION	2259:2268	arg1	promote					2361:2367	promote	2361:2367	promote bone regeneration in situ through mediation of the coupling processes of osteogenesis induction and osteoclast activity inhibition	2361:2498	CONCLUSION Our data suggests a promising application for the use of HAA scaffolds to load icariin and promote bone regeneration in situ through mediation of the coupling processes of osteogenesis induction and osteoclast activity inhibition.
31534334	8	14	theme	radiographic	1299:1310	arg1	images					1312:1317	radiographic images	1299:1317	radiographic images of the radius	1299:1331	Four and 12 weeks after the implantation of IC/HAA scaffolds in the bone defect, radiographic images of the radius were obtained and scored by using the Lane and Sandhu X-ray scoring system.
31534334	1	15	from	application	260:270	arg1	regeneration					280:291	bone regeneration	275:291	bone regeneration	275:291	OBJECTIVE Icariin (IC) promotes osteogenic differentiation, and it may be a potential small molecule drug for local application in bone regeneration.
31534334	2	16	theme	Icariin-loaded	294:307	arg1	scaffolds					359:367	Icariin-loaded hydroxyapatite/alginate (IC/HAA) porous composite scaffolds	294:367	Icariin-loaded hydroxyapatite/alginate (IC/HAA) porous composite scaffolds	294:367	Icariin-loaded hydroxyapatite/alginate (IC/HAA) porous composite scaffolds were designed in this study for the potential application of the sustainable release of icariin and subsequent bone regeneration.
31534334	4	17	from	properties	688:697	arg1	PBS					715:717	PBS	715:717	PBS	715:717	The mechanical properties, degradation in PBS and cytotoxicity on BMSCs were also evaluated by MTT assay, compression strength and calculation of weight remaining ratio, respectively.
31534334	4	17	from	properties	688:697	arg1	BMSCs					739:743	BMSCs	739:743	BMSCs	739:743	The mechanical properties, degradation in PBS and cytotoxicity on BMSCs were also evaluated by MTT assay, compression strength and calculation of weight remaining ratio, respectively.
31534334	5	18	theme	IC/HAA	891:896	arg1	scaffolds					898:906	IC/HAA scaffolds	891:906	IC/HAA scaffolds	891:906	Rabbit BMSCs were cocultured with IC/HAA scaffolds, and ALP activity and Alizarin Red staining were performed to evaluate osteogenic differentiation induction.
31534334	6	19	theme	mRNA	1021:1024	arg1	level					1049:1053	The mRNA and protein expression level	1017:1053	The mRNA and protein expression level of an osteogenic gene	1017:1075	The mRNA and protein expression level of an osteogenic gene was detected by RT-PCR and Western blotting, respectively.
31534334	15	20	theme	coupling	2420:2427	arg1	processes					2429:2437	the coupling processes	2416:2437	the coupling processes of osteogenesis induction and osteoclast activity inhibition	2416:2498	CONCLUSION Our data suggests a promising application for the use of HAA scaffolds to load icariin and promote bone regeneration in situ through mediation of the coupling processes of osteogenesis induction and osteoclast activity inhibition.
31534334	8	21	from	defect	1291:1296	arg1	implantation					1246:1257	the implantation	1242:1257	the implantation of IC/HAA scaffolds in the bone defect	1242:1296	Four and 12 weeks after the implantation of IC/HAA scaffolds in the bone defect, radiographic images of the radius were obtained and scored by using the Lane and Sandhu X-ray scoring system.
31534334	8	22	theme	scaffolds	1269:1277	arg1	implantation					1246:1257	the implantation	1242:1257	the implantation of IC/HAA scaffolds in the bone defect	1242:1296	Four and 12 weeks after the implantation of IC/HAA scaffolds in the bone defect, radiographic images of the radius were obtained and scored by using the Lane and Sandhu X-ray scoring system.
31534334	8	23	theme	radius	1326:1331	arg1	images					1312:1317	radiographic images	1299:1317	radiographic images of the radius	1299:1331	Four and 12 weeks after the implantation of IC/HAA scaffolds in the bone defect, radiographic images of the radius were obtained and scored by using the Lane and Sandhu X-ray scoring system.
31534334	13	24	theme	Wnt	2152:2154	arg1	genes					2174:2178	the Wnt signaling pathway genes	2148:2178	the Wnt signaling pathway genes	2148:2178	In vivo, rabbit radius bone defect experiments demonstrated that the IC/HAA scaffold exhibited better capacity for bone regeneration than HAA, and IC/HAA upregulated the relative expression levels of an osteogenic gene and the Wnt signaling pathway genes.
31534334	9	25	theme	signaling	1506:1514	arg1	genes					1524:1528	Wnt signaling pathway genes	1502:1528	Wnt signaling pathway genes	1502:1528	Tissue samples were also evaluated using H&E and Masson staining, and an osteogenic gene and Wnt signaling pathway genes were detected.
31534334	13	26	theme	pathway	2166:2172	arg1	genes					2174:2178	the Wnt signaling pathway genes	2148:2178	the Wnt signaling pathway genes	2148:2178	In vivo, rabbit radius bone defect experiments demonstrated that the IC/HAA scaffold exhibited better capacity for bone regeneration than HAA, and IC/HAA upregulated the relative expression levels of an osteogenic gene and the Wnt signaling pathway genes.
31534334	5	27	theme	osteogenic	979:988	arg1	induction					1006:1014	osteogenic differentiation induction	979:1014	osteogenic differentiation induction	979:1014	Rabbit BMSCs were cocultured with IC/HAA scaffolds, and ALP activity and Alizarin Red staining were performed to evaluate osteogenic differentiation induction.
31534334	11	28	theme	mechanical	1788:1797	arg1	properties					1799:1808	the macro/microstructure or mechanical properties	1760:1808	the macro/microstructure or mechanical properties of the scaffold	1760:1824	Our data indicated that the icariin was loaded in alginate scaffold without compromising the macro/microstructure or mechanical properties of the scaffold.
31534334	9	29	theme	H&E	1450:1452	arg1	staining					1465:1472	H&E and Masson staining	1450:1472	staining	1465:1472	Tissue samples were also evaluated using H&E and Masson staining, and an osteogenic gene and Wnt signaling pathway genes were detected.
31534334	13	30	theme	osteogenic	2128:2137	arg1	gene					2139:2142	an osteogenic gene	2125:2142	an osteogenic gene	2125:2142	In vivo, rabbit radius bone defect experiments demonstrated that the IC/HAA scaffold exhibited better capacity for bone regeneration than HAA, and IC/HAA upregulated the relative expression levels of an osteogenic gene and the Wnt signaling pathway genes.
31534334	5	31	theme	Red	939:941	arg1	staining					943:950	Alizarin Red staining	930:950	Alizarin Red staining	930:950	Rabbit BMSCs were cocultured with IC/HAA scaffolds, and ALP activity and Alizarin Red staining were performed to evaluate osteogenic differentiation induction.
31534334	6	32	theme	gene	1072:1075	arg1	level					1049:1053	The mRNA and protein expression level	1017:1053	The mRNA and protein expression level of an osteogenic gene	1017:1075	The mRNA and protein expression level of an osteogenic gene was detected by RT-PCR and Western blotting, respectively.
31534334	7	33	from	radius	1190:1195	arg1	models					1151:1156	In vivo animal models	1136:1156	In vivo animal models of critical bone defects in the radius of rabbit	1136:1205	In vivo animal models of critical bone defects in the radius of rabbit were used.
31534334	8	34	dep	Four	1218:1221	arg1	implantation					1246:1257	the implantation	1242:1257	the implantation of IC/HAA scaffolds in the bone defect	1242:1296	Four and 12 weeks after the implantation of IC/HAA scaffolds in the bone defect, radiographic images of the radius were obtained and scored by using the Lane and Sandhu X-ray scoring system.
31534334	13	35	theme	radius	1941:1946	arg1	experiments					1960:1970	rabbit radius bone defect experiments	1934:1970	rabbit radius bone defect experiments	1934:1970	In vivo, rabbit radius bone defect experiments demonstrated that the IC/HAA scaffold exhibited better capacity for bone regeneration than HAA, and IC/HAA upregulated the relative expression levels of an osteogenic gene and the Wnt signaling pathway genes.
31534334	15	36	theme	promising	2290:2298	arg1	application					2300:2310	a promising application	2288:2310	a promising application for the use of HAA scaffolds to load icariin	2288:2355	CONCLUSION Our data suggests a promising application for the use of HAA scaffolds to load icariin and promote bone regeneration in situ through mediation of the coupling processes of osteogenesis induction and osteoclast activity inhibition.
31534334	15	37	theme	inhibition	2489:2498	arg1	processes					2429:2437	the coupling processes	2416:2437	the coupling processes of osteogenesis induction and osteoclast activity inhibition	2416:2498	CONCLUSION Our data suggests a promising application for the use of HAA scaffolds to load icariin and promote bone regeneration in situ through mediation of the coupling processes of osteogenesis induction and osteoclast activity inhibition.
31534334	8	38	theme	Lane	1371:1374	arg1	system					1401:1406	the Lane and Sandhu X-ray scoring system	1367:1406	the Lane and Sandhu X-ray scoring system	1367:1406	Four and 12 weeks after the implantation of IC/HAA scaffolds in the bone defect, radiographic images of the radius were obtained and scored by using the Lane and Sandhu X-ray scoring system.
31534334	13	39	theme	defect	1953:1958	arg1	experiments					1960:1970	rabbit radius bone defect experiments	1934:1970	rabbit radius bone defect experiments	1934:1970	In vivo, rabbit radius bone defect experiments demonstrated that the IC/HAA scaffold exhibited better capacity for bone regeneration than HAA, and IC/HAA upregulated the relative expression levels of an osteogenic gene and the Wnt signaling pathway genes.
31534334	11	40	theme	scaffold	1817:1824	arg1	properties					1799:1808	the macro/microstructure or mechanical properties	1760:1808	the macro/microstructure or mechanical properties of the scaffold	1760:1824	Our data indicated that the icariin was loaded in alginate scaffold without compromising the macro/microstructure or mechanical properties of the scaffold.
31534334	0	41	theme	process	93:99	arg1	regulation					66:75	the regulation	62:75	the regulation of the coupling process of osteogenesis and osteoclastic activity	62:141	Icariin-loaded porous scaffolds for bone regeneration through the regulation of the coupling process of osteogenesis and osteoclastic activity.
31534334	13	42	theme	expression	2104:2113	arg1	levels					2115:2120	the relative expression levels	2091:2120	the relative expression levels of an osteogenic gene and the Wnt signaling pathway genes	2091:2178	In vivo, rabbit radius bone defect experiments demonstrated that the IC/HAA scaffold exhibited better capacity for bone regeneration than HAA, and IC/HAA upregulated the relative expression levels of an osteogenic gene and the Wnt signaling pathway genes.
31534334	1	43	theme	OBJECTIVE	144:152	arg1	IC					163:164	IC	163:164	IC	163:164	OBJECTIVE Icariin (IC) promotes osteogenic differentiation, and it may be a potential small molecule drug for local application in bone regeneration.
31534334	1	43	theme	OBJECTIVE	144:152	arg1	Icariin					154:160	OBJECTIVE Icariin	144:160	OBJECTIVE Icariin (IC)	144:165	OBJECTIVE Icariin (IC) promotes osteogenic differentiation, and it may be a potential small molecule drug for local application in bone regeneration.
31534334	4	44	theme	remaining	826:834	arg1	ratio					836:840	weight remaining ratio	819:840	weight remaining ratio	819:840	The mechanical properties, degradation in PBS and cytotoxicity on BMSCs were also evaluated by MTT assay, compression strength and calculation of weight remaining ratio, respectively.
31534334	2	45	theme	potential	405:413	arg1	application					415:425	the potential application	401:425	the potential application of the sustainable release of icariin and subsequent bone regeneration	401:496	Icariin-loaded hydroxyapatite/alginate (IC/HAA) porous composite scaffolds were designed in this study for the potential application of the sustainable release of icariin and subsequent bone regeneration.
31534334	0	46	theme	osteogenesis	104:115	arg1	process					93:99	the coupling process	80:99	the coupling process of osteogenesis and osteoclastic activity	80:141	Icariin-loaded porous scaffolds for bone regeneration through the regulation of the coupling process of osteogenesis and osteoclastic activity.
31534334	15	47	theme	induction	2455:2463	arg1	processes					2429:2437	the coupling processes	2416:2437	the coupling processes of osteogenesis induction and osteoclast activity inhibition	2416:2498	CONCLUSION Our data suggests a promising application for the use of HAA scaffolds to load icariin and promote bone regeneration in situ through mediation of the coupling processes of osteogenesis induction and osteoclast activity inhibition.
31534334	4	48	theme	compression	779:789	arg1	strength					791:798	compression strength	779:798	compression strength	779:798	The mechanical properties, degradation in PBS and cytotoxicity on BMSCs were also evaluated by MTT assay, compression strength and calculation of weight remaining ratio, respectively.
31534334	0	49	theme	Icariin-loaded	0:13	arg1	porous					15:20	Icariin-loaded porous	0:20	Icariin-loaded porous	0:20	Icariin-loaded porous scaffolds for bone regeneration through the regulation of the coupling process of osteogenesis and osteoclastic activity.
31534334	15	50	theme	scaffolds	2331:2339	arg1	use					2320:2322	the use	2316:2322	the use of HAA scaffolds to load icariin	2316:2355	CONCLUSION Our data suggests a promising application for the use of HAA scaffolds to load icariin and promote bone regeneration in situ through mediation of the coupling processes of osteogenesis induction and osteoclast activity inhibition.
31534334	2	51	theme	sustainable	434:444	arg1	release					446:452	the sustainable release	430:452	the sustainable release of icariin and subsequent bone regeneration	430:496	Icariin-loaded hydroxyapatite/alginate (IC/HAA) porous composite scaffolds were designed in this study for the potential application of the sustainable release of icariin and subsequent bone regeneration.
31534334	6	52	theme	Western	1104:1110	arg1	blotting					1112:1119	Western blotting	1104:1119	Western blotting	1104:1119	The mRNA and protein expression level of an osteogenic gene was detected by RT-PCR and Western blotting, respectively.
31534334	0	53	theme	bone	36:39	arg1	regeneration					41:52	bone regeneration	36:52	bone regeneration	36:52	Icariin-loaded porous scaffolds for bone regeneration through the regulation of the coupling process of osteogenesis and osteoclastic activity.
31534334	7	54	theme	rabbit	1200:1205	arg1	radius					1190:1195	the radius	1186:1195	the radius of rabbit	1186:1205	In vivo animal models of critical bone defects in the radius of rabbit were used.
31534334	2	55	theme	bone	480:483	arg1	regeneration					485:496	subsequent bone regeneration	469:496	subsequent bone regeneration	469:496	Icariin-loaded hydroxyapatite/alginate (IC/HAA) porous composite scaffolds were designed in this study for the potential application of the sustainable release of icariin and subsequent bone regeneration.
31534334	11	56	theme	alginate	1721:1728	arg1	scaffold					1730:1737	alginate scaffold	1721:1737	alginate scaffold	1721:1737	Our data indicated that the icariin was loaded in alginate scaffold without compromising the macro/microstructure or mechanical properties of the scaffold.
31534334	3	57	theme	icariin-loaded	510:523	arg1	scaffold					566:573	An icariin-loaded hydroxyapatite/alginate porous composite scaffold	507:573	An icariin-loaded hydroxyapatite/alginate porous composite scaffold	507:573	METHODS An icariin-loaded hydroxyapatite/alginate porous composite scaffold was prepared and characterized by SEM and HPLC for morphology and release behavior, respectively.
31534334	13	58	theme	relative	2095:2102	arg1	levels					2115:2120	the relative expression levels	2091:2120	the relative expression levels of an osteogenic gene and the Wnt signaling pathway genes	2091:2178	In vivo, rabbit radius bone defect experiments demonstrated that the IC/HAA scaffold exhibited better capacity for bone regeneration than HAA, and IC/HAA upregulated the relative expression levels of an osteogenic gene and the Wnt signaling pathway genes.
31534334	2	59	theme	porous	342:347	arg1	scaffolds					359:367	Icariin-loaded hydroxyapatite/alginate (IC/HAA) porous composite scaffolds	294:367	Icariin-loaded hydroxyapatite/alginate (IC/HAA) porous composite scaffolds	294:367	Icariin-loaded hydroxyapatite/alginate (IC/HAA) porous composite scaffolds were designed in this study for the potential application of the sustainable release of icariin and subsequent bone regeneration.
31534334	9	60	theme	Tissue	1409:1414	arg1	samples					1416:1422	Tissue samples	1409:1422	Tissue samples	1409:1422	Tissue samples were also evaluated using H&E and Masson staining, and an osteogenic gene and Wnt signaling pathway genes were detected.
31534334	5	61	theme	ALP	913:915	arg1	activity					917:924	ALP activity	913:924	ALP activity	913:924	Rabbit BMSCs were cocultured with IC/HAA scaffolds, and ALP activity and Alizarin Red staining were performed to evaluate osteogenic differentiation induction.
31534334	1	62	theme	small	230:234	arg1	it					208:209	it	208:209	it	208:209	OBJECTIVE Icariin (IC) promotes osteogenic differentiation, and it may be a potential small molecule drug for local application in bone regeneration.
31534334	1	62	theme	small	230:234	arg1	drug					245:248	a potential small molecule drug	218:248	a potential small molecule drug for local application in bone regeneration	218:291	OBJECTIVE Icariin (IC) promotes osteogenic differentiation, and it may be a potential small molecule drug for local application in bone regeneration.
31534334	10	63	dep	RESULTS	1545:1551	arg1	fabricated					1630:1639	fabricated	1630:1639	was fabricated using a freeze-drying method	1626:1668	RESULTS A hydroxyapatite/alginate (HAA) porous composite scaffold-loaded icariin was fabricated using a freeze-drying method.
31534334	7	64	theme	defects	1175:1181	arg1	models					1151:1156	In vivo animal models	1136:1156	In vivo animal models of critical bone defects in the radius of rabbit	1136:1205	In vivo animal models of critical bone defects in the radius of rabbit were used.
31534334	3	65	theme	porous	549:554	arg1	scaffold					566:573	An icariin-loaded hydroxyapatite/alginate porous composite scaffold	507:573	An icariin-loaded hydroxyapatite/alginate porous composite scaffold	507:573	METHODS An icariin-loaded hydroxyapatite/alginate porous composite scaffold was prepared and characterized by SEM and HPLC for morphology and release behavior, respectively.
31534334	10	66	theme	scaffold-loaded	1602:1616	arg1	icariin					1618:1624	A hydroxyapatite/alginate (HAA) porous composite scaffold-loaded icariin	1553:1624	A hydroxyapatite/alginate (HAA) porous composite scaffold-loaded icariin	1553:1624	RESULTS A hydroxyapatite/alginate (HAA) porous composite scaffold-loaded icariin was fabricated using a freeze-drying method.
31534334	7	67	theme	critical	1161:1168	arg1	defects					1175:1181	critical bone defects	1161:1181	critical bone defects in the radius of rabbit	1161:1205	In vivo animal models of critical bone defects in the radius of rabbit were used.
31534334	1	68	theme	local	254:258	arg1	application					260:270	local application	254:270	local application in bone regeneration	254:291	OBJECTIVE Icariin (IC) promotes osteogenic differentiation, and it may be a potential small molecule drug for local application in bone regeneration.
31534334	8	69	from	scaffolds	1269:1277	arg1	defect					1291:1296	the bone defect	1282:1296	the bone defect	1282:1296	Four and 12 weeks after the implantation of IC/HAA scaffolds in the bone defect, radiographic images of the radius were obtained and scored by using the Lane and Sandhu X-ray scoring system.
31534334	10	70	theme	porous	1585:1590	arg1	icariin					1618:1624	A hydroxyapatite/alginate (HAA) porous composite scaffold-loaded icariin	1553:1624	A hydroxyapatite/alginate (HAA) porous composite scaffold-loaded icariin	1553:1624	RESULTS A hydroxyapatite/alginate (HAA) porous composite scaffold-loaded icariin was fabricated using a freeze-drying method.
31534334	3	71	dep	morphology	626:635	arg1	behavior					649:656	behavior	649:656	behavior	649:656	METHODS An icariin-loaded hydroxyapatite/alginate porous composite scaffold was prepared and characterized by SEM and HPLC for morphology and release behavior, respectively.
31534334	6	72	theme	expression	1038:1047	arg1	level					1049:1053	The mRNA and protein expression level	1017:1053	The mRNA and protein expression level of an osteogenic gene	1017:1075	The mRNA and protein expression level of an osteogenic gene was detected by RT-PCR and Western blotting, respectively.
31534334	8	73	theme	bone	1286:1289	arg1	defect					1291:1296	the bone defect	1282:1296	the bone defect	1282:1296	Four and 12 weeks after the implantation of IC/HAA scaffolds in the bone defect, radiographic images of the radius were obtained and scored by using the Lane and Sandhu X-ray scoring system.
31534334	14	74	theme	osteoclast	2230:2239	arg1	activity					2241:2248	osteoclast activity	2230:2248	osteoclast activity	2230:2248	Most notably, the IC/HAA scaffold also inhibited osteoclast activity in vivo.
31534334	0	75	theme	activity	134:141	arg1	process					93:99	the coupling process	80:99	the coupling process of osteogenesis and osteoclastic activity	80:141	Icariin-loaded porous scaffolds for bone regeneration through the regulation of the coupling process of osteogenesis and osteoclastic activity.
31534334	13	76	theme	better	2020:2025	arg1	capacity					2027:2034	better capacity	2020:2034	better capacity for bone regeneration	2020:2056	In vivo, rabbit radius bone defect experiments demonstrated that the IC/HAA scaffold exhibited better capacity for bone regeneration than HAA, and IC/HAA upregulated the relative expression levels of an osteogenic gene and the Wnt signaling pathway genes.
31534334	4	77	theme	mechanical	677:686	arg1	properties					688:697	The mechanical properties	673:697	The mechanical properties	673:697	The mechanical properties, degradation in PBS and cytotoxicity on BMSCs were also evaluated by MTT assay, compression strength and calculation of weight remaining ratio, respectively.
31534334	2	78	theme	composite	349:357	arg1	scaffolds					359:367	Icariin-loaded hydroxyapatite/alginate (IC/HAA) porous composite scaffolds	294:367	Icariin-loaded hydroxyapatite/alginate (IC/HAA) porous composite scaffolds	294:367	Icariin-loaded hydroxyapatite/alginate (IC/HAA) porous composite scaffolds were designed in this study for the potential application of the sustainable release of icariin and subsequent bone regeneration.
31534334	15	79	theme	osteogenesis	2442:2453	arg1	induction					2455:2463	osteogenesis induction	2442:2463	osteogenesis induction	2442:2463	CONCLUSION Our data suggests a promising application for the use of HAA scaffolds to load icariin and promote bone regeneration in situ through mediation of the coupling processes of osteogenesis induction and osteoclast activity inhibition.
31534334	10	80	theme	freeze-drying	1649:1661	arg1	method					1663:1668	a freeze-drying method	1647:1668	a freeze-drying method	1647:1668	RESULTS A hydroxyapatite/alginate (HAA) porous composite scaffold-loaded icariin was fabricated using a freeze-drying method.
31534334	15	81	theme	processes	2429:2437	arg1	mediation					2403:2411	mediation	2403:2411	mediation of the coupling processes of osteogenesis induction and osteoclast activity inhibition	2403:2498	CONCLUSION Our data suggests a promising application for the use of HAA scaffolds to load icariin and promote bone regeneration in situ through mediation of the coupling processes of osteogenesis induction and osteoclast activity inhibition.
31534334	9	82	theme	Wnt	1502:1504	arg1	genes					1524:1528	Wnt signaling pathway genes	1502:1528	Wnt signaling pathway genes	1502:1528	Tissue samples were also evaluated using H&E and Masson staining, and an osteogenic gene and Wnt signaling pathway genes were detected.
31534334	8	83	theme	IC/HAA	1262:1267	arg1	scaffolds					1269:1277	IC/HAA scaffolds	1262:1277	IC/HAA scaffolds in the bone defect	1262:1296	Four and 12 weeks after the implantation of IC/HAA scaffolds in the bone defect, radiographic images of the radius were obtained and scored by using the Lane and Sandhu X-ray scoring system.
31534334	13	84	theme	signaling	2156:2164	arg1	genes					2174:2178	the Wnt signaling pathway genes	2148:2178	the Wnt signaling pathway genes	2148:2178	In vivo, rabbit radius bone defect experiments demonstrated that the IC/HAA scaffold exhibited better capacity for bone regeneration than HAA, and IC/HAA upregulated the relative expression levels of an osteogenic gene and the Wnt signaling pathway genes.
31534334	9	85	theme	pathway	1516:1522	arg1	genes					1524:1528	Wnt signaling pathway genes	1502:1528	Wnt signaling pathway genes	1502:1528	Tissue samples were also evaluated using H&E and Masson staining, and an osteogenic gene and Wnt signaling pathway genes were detected.
31534334	4	86	theme	MTT	768:770	arg1	assay					772:776	MTT assay	768:776	MTT assay	768:776	The mechanical properties, degradation in PBS and cytotoxicity on BMSCs were also evaluated by MTT assay, compression strength and calculation of weight remaining ratio, respectively.
31534334	13	87	theme	genes	2174:2178	arg1	levels					2115:2120	the relative expression levels	2091:2120	the relative expression levels of an osteogenic gene and the Wnt signaling pathway genes	2091:2178	In vivo, rabbit radius bone defect experiments demonstrated that the IC/HAA scaffold exhibited better capacity for bone regeneration than HAA, and IC/HAA upregulated the relative expression levels of an osteogenic gene and the Wnt signaling pathway genes.
31534334	5	88	theme	Alizarin	930:937	arg1	staining					943:950	Alizarin Red staining	930:950	Alizarin Red staining	930:950	Rabbit BMSCs were cocultured with IC/HAA scaffolds, and ALP activity and Alizarin Red staining were performed to evaluate osteogenic differentiation induction.
31534334	13	89	theme	rabbit	1934:1939	arg1	experiments					1960:1970	rabbit radius bone defect experiments	1934:1970	rabbit radius bone defect experiments	1934:1970	In vivo, rabbit radius bone defect experiments demonstrated that the IC/HAA scaffold exhibited better capacity for bone regeneration than HAA, and IC/HAA upregulated the relative expression levels of an osteogenic gene and the Wnt signaling pathway genes.
31534334	0	90	theme	coupling	84:91	arg1	process					93:99	the coupling process	80:99	the coupling process of osteogenesis and osteoclastic activity	80:141	Icariin-loaded porous scaffolds for bone regeneration through the regulation of the coupling process of osteogenesis and osteoclastic activity.
31534334	13	91	theme	gene	2139:2142	arg1	levels					2115:2120	the relative expression levels	2091:2120	the relative expression levels of an osteogenic gene and the Wnt signaling pathway genes	2091:2178	In vivo, rabbit radius bone defect experiments demonstrated that the IC/HAA scaffold exhibited better capacity for bone regeneration than HAA, and IC/HAA upregulated the relative expression levels of an osteogenic gene and the Wnt signaling pathway genes.
31534334	15	92	theme	osteoclast	2469:2478	arg1	inhibition					2489:2498	osteoclast activity inhibition	2469:2498	osteoclast activity inhibition	2469:2498	CONCLUSION Our data suggests a promising application for the use of HAA scaffolds to load icariin and promote bone regeneration in situ through mediation of the coupling processes of osteogenesis induction and osteoclast activity inhibition.
31534334	6	93	theme	osteogenic	1061:1070	arg1	gene					1072:1075	an osteogenic gene	1058:1075	an osteogenic gene	1058:1075	The mRNA and protein expression level of an osteogenic gene was detected by RT-PCR and Western blotting, respectively.
31534334	7	94	theme	In	1136:1137	arg1	models					1151:1156	In vivo animal models	1136:1156	In vivo animal models of critical bone defects in the radius of rabbit	1136:1205	In vivo animal models of critical bone defects in the radius of rabbit were used.
31534334	13	95	theme	bone	1948:1951	arg1	experiments					1960:1970	rabbit radius bone defect experiments	1934:1970	rabbit radius bone defect experiments	1934:1970	In vivo, rabbit radius bone defect experiments demonstrated that the IC/HAA scaffold exhibited better capacity for bone regeneration than HAA, and IC/HAA upregulated the relative expression levels of an osteogenic gene and the Wnt signaling pathway genes.
31534334	3	96	dep	METHODS	499:505	arg1	characterized					592:604	characterized	592:604	characterized	592:604	METHODS An icariin-loaded hydroxyapatite/alginate porous composite scaffold was prepared and characterized by SEM and HPLC for morphology and release behavior, respectively.
31534334	3	96	dep	METHODS	499:505	arg1	prepared					579:586	prepared	579:586	prepared	579:586	METHODS An icariin-loaded hydroxyapatite/alginate porous composite scaffold was prepared and characterized by SEM and HPLC for morphology and release behavior, respectively.
31534334	15	97	theme	activity	2480:2487	arg1	inhibition					2489:2498	osteoclast activity inhibition	2469:2498	osteoclast activity inhibition	2469:2498	CONCLUSION Our data suggests a promising application for the use of HAA scaffolds to load icariin and promote bone regeneration in situ through mediation of the coupling processes of osteogenesis induction and osteoclast activity inhibition.
31534334	9	98	theme	osteogenic	1482:1491	arg1	gene					1493:1496	an osteogenic gene	1479:1496	an osteogenic gene	1479:1496	Tissue samples were also evaluated using H&E and Masson staining, and an osteogenic gene and Wnt signaling pathway genes were detected.
31534334	4	99	theme	weight	819:824	arg1	ratio					836:840	weight remaining ratio	819:840	weight remaining ratio	819:840	The mechanical properties, degradation in PBS and cytotoxicity on BMSCs were also evaluated by MTT assay, compression strength and calculation of weight remaining ratio, respectively.
31534334	5	100	theme	Rabbit	857:862	arg1	BMSCs					864:868	Rabbit BMSCs	857:868	Rabbit BMSCs	857:868	Rabbit BMSCs were cocultured with IC/HAA scaffolds, and ALP activity and Alizarin Red staining were performed to evaluate osteogenic differentiation induction.
31534334	10	101	theme	composite	1592:1600	arg1	icariin					1618:1624	A hydroxyapatite/alginate (HAA) porous composite scaffold-loaded icariin	1553:1624	A hydroxyapatite/alginate (HAA) porous composite scaffold-loaded icariin	1553:1624	RESULTS A hydroxyapatite/alginate (HAA) porous composite scaffold-loaded icariin was fabricated using a freeze-drying method.
31534334	5	102	theme	differentiation	990:1004	arg1	induction					1006:1014	osteogenic differentiation induction	979:1014	osteogenic differentiation induction	979:1014	Rabbit BMSCs were cocultured with IC/HAA scaffolds, and ALP activity and Alizarin Red staining were performed to evaluate osteogenic differentiation induction.
31534334	2	103	theme	icariin	457:463	arg1	release					446:452	the sustainable release	430:452	the sustainable release of icariin and subsequent bone regeneration	430:496	Icariin-loaded hydroxyapatite/alginate (IC/HAA) porous composite scaffolds were designed in this study for the potential application of the sustainable release of icariin and subsequent bone regeneration.
31534334	8	104	theme	X-ray	1387:1391	arg1	system					1401:1406	the Lane and Sandhu X-ray scoring system	1367:1406	the Lane and Sandhu X-ray scoring system	1367:1406	Four and 12 weeks after the implantation of IC/HAA scaffolds in the bone defect, radiographic images of the radius were obtained and scored by using the Lane and Sandhu X-ray scoring system.
31534334	1	105	theme	bone	275:278	arg1	regeneration					280:291	bone regeneration	275:291	bone regeneration	275:291	OBJECTIVE Icariin (IC) promotes osteogenic differentiation, and it may be a potential small molecule drug for local application in bone regeneration.
31534334	7	106	used	used	1212:1215	arg2	models					1151:1156	In vivo animal models	1136:1156	In vivo animal models of critical bone defects in the radius of rabbit	1136:1205	In vivo animal models of critical bone defects in the radius of rabbit were used.
31534334	2	107	theme	release	446:452	arg1	application					415:425	the potential application	401:425	the potential application of the sustainable release of icariin and subsequent bone regeneration	401:496	Icariin-loaded hydroxyapatite/alginate (IC/HAA) porous composite scaffolds were designed in this study for the potential application of the sustainable release of icariin and subsequent bone regeneration.
31534334	7	108	from	models	1151:1156	arg1	radius					1190:1195	the radius	1186:1195	the radius of rabbit	1186:1205	In vivo animal models of critical bone defects in the radius of rabbit were used.
31534334	8	109	theme	Sandhu	1380:1385	arg1	system					1401:1406	the Lane and Sandhu X-ray scoring system	1367:1406	the Lane and Sandhu X-ray scoring system	1367:1406	Four and 12 weeks after the implantation of IC/HAA scaffolds in the bone defect, radiographic images of the radius were obtained and scored by using the Lane and Sandhu X-ray scoring system.
31534334	15	110	theme	HAA	2327:2329	arg1	scaffolds					2331:2339	HAA scaffolds	2327:2339	HAA scaffolds	2327:2339	CONCLUSION Our data suggests a promising application for the use of HAA scaffolds to load icariin and promote bone regeneration in situ through mediation of the coupling processes of osteogenesis induction and osteoclast activity inhibition.
31534334	2	111	theme	regeneration	485:496	arg1	release					446:452	the sustainable release	430:452	the sustainable release of icariin and subsequent bone regeneration	430:496	Icariin-loaded hydroxyapatite/alginate (IC/HAA) porous composite scaffolds were designed in this study for the potential application of the sustainable release of icariin and subsequent bone regeneration.
31534334	15	112	theme	bone	2369:2372	arg1	regeneration					2374:2385	bone regeneration	2369:2385	bone regeneration	2369:2385	CONCLUSION Our data suggests a promising application for the use of HAA scaffolds to load icariin and promote bone regeneration in situ through mediation of the coupling processes of osteogenesis induction and osteoclast activity inhibition.
31534334	2	113	theme	subsequent	469:478	arg1	regeneration					485:496	subsequent bone regeneration	469:496	subsequent bone regeneration	469:496	Icariin-loaded hydroxyapatite/alginate (IC/HAA) porous composite scaffolds were designed in this study for the potential application of the sustainable release of icariin and subsequent bone regeneration.
31534334	7	114	from	defects	1175:1181	arg1	radius					1190:1195	the radius	1186:1195	the radius of rabbit	1186:1205	In vivo animal models of critical bone defects in the radius of rabbit were used.
31534334	11	115	theme	macro/microstructure	1764:1783	arg1	properties					1799:1808	the macro/microstructure or mechanical properties	1760:1808	the macro/microstructure or mechanical properties of the scaffold	1760:1824	Our data indicated that the icariin was loaded in alginate scaffold without compromising the macro/microstructure or mechanical properties of the scaffold.
31534334	3	116	theme	hydroxyapatite/alginate	525:547	arg1	scaffold					566:573	An icariin-loaded hydroxyapatite/alginate porous composite scaffold	507:573	An icariin-loaded hydroxyapatite/alginate porous composite scaffold	507:573	METHODS An icariin-loaded hydroxyapatite/alginate porous composite scaffold was prepared and characterized by SEM and HPLC for morphology and release behavior, respectively.
31534334	15	117	theme	load	2344:2347	arg1	icariin					2349:2355	load icariin	2344:2355	load icariin	2344:2355	CONCLUSION Our data suggests a promising application for the use of HAA scaffolds to load icariin and promote bone regeneration in situ through mediation of the coupling processes of osteogenesis induction and osteoclast activity inhibition.
31534334	12	118	theme	rBMSCs	1877:1882	arg1	proliferation					1860:1872	the proliferation	1856:1872	the proliferation of rBMSCs	1856:1882	Notably, the IC/HAA promoted the proliferation of rBMSCs without exerting cytotoxicity on rBMSCs.
31534334	4	119	theme	ratio	836:840	arg1	strength					791:798	compression strength	779:798	compression strength	779:798	The mechanical properties, degradation in PBS and cytotoxicity on BMSCs were also evaluated by MTT assay, compression strength and calculation of weight remaining ratio, respectively.
31534334	4	119	theme	ratio	836:840	arg1	calculation					804:814	calculation	804:814	calculation of weight remaining ratio	804:840	The mechanical properties, degradation in PBS and cytotoxicity on BMSCs were also evaluated by MTT assay, compression strength and calculation of weight remaining ratio, respectively.
31534334	4	119	theme	ratio	836:840	arg1	assay					772:776	MTT assay	768:776	MTT assay	768:776	The mechanical properties, degradation in PBS and cytotoxicity on BMSCs were also evaluated by MTT assay, compression strength and calculation of weight remaining ratio, respectively.
31534334	1	120	theme	potential	220:228	arg1	it					208:209	it	208:209	it	208:209	OBJECTIVE Icariin (IC) promotes osteogenic differentiation, and it may be a potential small molecule drug for local application in bone regeneration.
31534334	1	120	theme	potential	220:228	arg1	drug					245:248	a potential small molecule drug	218:248	a potential small molecule drug for local application in bone regeneration	218:291	OBJECTIVE Icariin (IC) promotes osteogenic differentiation, and it may be a potential small molecule drug for local application in bone regeneration.
31534334	0	121	theme	osteoclastic	121:132	arg1	activity					134:141	osteoclastic activity	121:141	osteoclastic activity	121:141	Icariin-loaded porous scaffolds for bone regeneration through the regulation of the coupling process of osteogenesis and osteoclastic activity.
31534334	14	122	theme	IC/HAA	2199:2204	arg1	scaffold					2206:2213	the IC/HAA scaffold	2195:2213	the IC/HAA scaffold	2195:2213	Most notably, the IC/HAA scaffold also inhibited osteoclast activity in vivo.
31534334	1	123	theme	molecule	236:243	arg1	it					208:209	it	208:209	it	208:209	OBJECTIVE Icariin (IC) promotes osteogenic differentiation, and it may be a potential small molecule drug for local application in bone regeneration.
31534334	1	123	theme	molecule	236:243	arg1	drug					245:248	a potential small molecule drug	218:248	a potential small molecule drug for local application in bone regeneration	218:291	OBJECTIVE Icariin (IC) promotes osteogenic differentiation, and it may be a potential small molecule drug for local application in bone regeneration.
31665101	9	0	theme	polar	1153:1157	arg1	lipids					1159:1164	the major polar lipids	1143:1164	the major polar lipids	1143:1164	The strain contained MK-8(H4) as the predominant respiratory quinone, phosphatidylethanolamine and diphosphatidylglycerol as the major polar lipids, rhamnose, ribose and glucose as the major whole-cell sugars, and meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
31665101	9	0	theme	polar	1153:1157	arg1	MK-8					1039:1042	MK-8	1039:1042	MK-8(H4)	1039:1046	The strain contained MK-8(H4) as the predominant respiratory quinone, phosphatidylethanolamine and diphosphatidylglycerol as the major polar lipids, rhamnose, ribose and glucose as the major whole-cell sugars, and meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
31665101	4	1	theme	0-5 	413:416	arg1	%					417:417	%	417:417	%	417:417	Growth occurred at 4-28 °C, at pH 5.0-9.0 and in the presence of 0-5 % (w/v) NaCl.
31665101	5	2	theme	genus	545:549	arg1	Nakamurella					551:561	the genus Nakamurella	541:561	the genus Nakamurella	541:561	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain S14-144T formed a lineage within the genus Nakamurella and shared the highest 16S rRNA gene sequence similarity with Nakamurella deserti 12Sc4-1T (96.5 %) and Nakamurella silvestrisS20-107T (96.4 %).
31665101	5	3	theme	Phylogenetic	431:442	arg1	analysis					444:451	Phylogenetic analysis	431:451	Phylogenetic analysis based on 16S rRNA gene sequences	431:484	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain S14-144T formed a lineage within the genus Nakamurella and shared the highest 16S rRNA gene sequence similarity with Nakamurella deserti 12Sc4-1T (96.5 %) and Nakamurella silvestrisS20-107T (96.4 %).
31665101	10	4	theme	Nakamurella	1498:1508	arg1	sp					1521:1522	the name Nakamurella antarctica sp	1489:1522	the name Nakamurella antarctica sp	1489:1522	On the basis of the phylogenetic, phenotypic and chemotaxonomic analysis, strain S14-144T is considered to represent a novel species of the genus Nakamurella, for which the name Nakamurella antarctica sp.
31665101	8	5	theme	fatty	919:923	arg1	acids					925:929	The major cellular fatty acids	900:929	The major cellular fatty acids of strain S14-144T	900:948	The major cellular fatty acids of strain S14-144T were summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c) and C16 : 0.
31665101	6	6	theme	strain	797:802	arg1	genomes					758:764	the genomes	754:764	the genomes of strain 14-144T and the type strain of the species, N. deserti,	754:830	The average nucleotide identity value between the genomes of strain 14-144T and the type strain of the species, N. deserti, was 72.0 % .
31665101	10	7	theme	name	1493:1496	arg1	sp					1521:1522	the name Nakamurella antarctica sp	1489:1522	the name Nakamurella antarctica sp	1489:1522	On the basis of the phylogenetic, phenotypic and chemotaxonomic analysis, strain S14-144T is considered to represent a novel species of the genus Nakamurella, for which the name Nakamurella antarctica sp.
31665101	5	8	theme	Nakamurella	625:635	arg1	12Sc4-1T					645:652	Nakamurella deserti 12Sc4-1T	625:652	Nakamurella deserti 12Sc4-1T (96.5 %)	625:661	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain S14-144T formed a lineage within the genus Nakamurella and shared the highest 16S rRNA gene sequence similarity with Nakamurella deserti 12Sc4-1T (96.5 %) and Nakamurella silvestrisS20-107T (96.4 %).
31665101	5	8	theme	Nakamurella	625:635	arg1	%					660:660	96.5 %	655:660	96.5 %	655:660	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain S14-144T formed a lineage within the genus Nakamurella and shared the highest 16S rRNA gene sequence similarity with Nakamurella deserti 12Sc4-1T (96.5 %) and Nakamurella silvestrisS20-107T (96.4 %).
31665101	2	9	theme	bacterial	101:109	arg1	strain					111:116	A pale-yellow bacterial strain	87:116	A pale-yellow bacterial strain	87:116	A pale-yellow bacterial strain, designated S14-144T, was isolated from tundra soil sampled near the Antarctic Peninsula, South Shetland Islands (62° 22' 34″ S, 59° 42' 34″ W).
31665101	5	10	theme	strain	501:506	arg1	S14-144T					508:515	strain S14-144T	501:515	strain S14-144T	501:515	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain S14-144T formed a lineage within the genus Nakamurella and shared the highest 16S rRNA gene sequence similarity with Nakamurella deserti 12Sc4-1T (96.5 %) and Nakamurella silvestrisS20-107T (96.4 %).
31665101	1	11	theme	Antarctica	47:56	arg1	soil					81:84	Antarctica South Shetland Islands soil	47:84	Antarctica South Shetland Islands soil	47:84	nov., isolated from Antarctica South Shetland Islands soil.
31665101	9	12	theme	respiratory	1067:1077	arg1	MK-8					1039:1042	MK-8	1039:1042	MK-8(H4)	1039:1046	The strain contained MK-8(H4) as the predominant respiratory quinone, phosphatidylethanolamine and diphosphatidylglycerol as the major polar lipids, rhamnose, ribose and glucose as the major whole-cell sugars, and meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
31665101	9	12	theme	respiratory	1067:1077	arg1	quinone					1079:1085	the predominant respiratory quinone	1051:1085	the predominant respiratory quinone	1051:1085	The strain contained MK-8(H4) as the predominant respiratory quinone, phosphatidylethanolamine and diphosphatidylglycerol as the major polar lipids, rhamnose, ribose and glucose as the major whole-cell sugars, and meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
31665101	5	13	theme	deserti	637:643	arg1	12Sc4-1T					645:652	Nakamurella deserti 12Sc4-1T	625:652	Nakamurella deserti 12Sc4-1T (96.5 %)	625:661	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain S14-144T formed a lineage within the genus Nakamurella and shared the highest 16S rRNA gene sequence similarity with Nakamurella deserti 12Sc4-1T (96.5 %) and Nakamurella silvestrisS20-107T (96.4 %).
31665101	5	13	theme	deserti	637:643	arg1	%					660:660	96.5 %	655:660	96.5 %	655:660	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain S14-144T formed a lineage within the genus Nakamurella and shared the highest 16S rRNA gene sequence similarity with Nakamurella deserti 12Sc4-1T (96.5 %) and Nakamurella silvestrisS20-107T (96.4 %).
31665101	12	14	theme	AB	1579:1580	arg1	39796T					1596:1601	=CCTCC AB 2015345T=KCTC 39796T	1572:1601	=CCTCC AB 2015345T=KCTC 39796T	1572:1601	The type strain is S14-144T (=CCTCC AB 2015345T=KCTC 39796T).
31665101	12	14	theme	AB	1579:1580	arg1	S14-144T					1562:1569	S14-144T	1562:1569	S14-144T (=CCTCC AB 2015345T=KCTC 39796T)	1562:1602	The type strain is S14-144T (=CCTCC AB 2015345T=KCTC 39796T).
31665101	1	15	theme	South	58:62	arg1	soil					81:84	Antarctica South Shetland Islands soil	47:84	Antarctica South Shetland Islands soil	47:84	nov., isolated from Antarctica South Shetland Islands soil.
31665101	9	16	theme	whole-cell	1209:1218	arg1	sugars					1220:1225	the major whole-cell sugars	1199:1225	the major whole-cell sugars	1199:1225	The strain contained MK-8(H4) as the predominant respiratory quinone, phosphatidylethanolamine and diphosphatidylglycerol as the major polar lipids, rhamnose, ribose and glucose as the major whole-cell sugars, and meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
31665101	10	17	dep	analysis	1384:1391	arg1	the					1323:1325	the	1323:1325	the	1323:1325	On the basis of the phylogenetic, phenotypic and chemotaxonomic analysis, strain S14-144T is considered to represent a novel species of the genus Nakamurella, for which the name Nakamurella antarctica sp.
31665101	10	17	dep	analysis	1384:1391	arg1	basis					1327:1331	basis	1327:1331	basis	1327:1331	On the basis of the phylogenetic, phenotypic and chemotaxonomic analysis, strain S14-144T is considered to represent a novel species of the genus Nakamurella, for which the name Nakamurella antarctica sp.
31665101	12	18	theme	type	1547:1550	arg1	S14-144T					1562:1569	S14-144T	1562:1569	S14-144T (=CCTCC AB 2015345T=KCTC 39796T)	1562:1602	The type strain is S14-144T (=CCTCC AB 2015345T=KCTC 39796T).
31665101	12	18	theme	type	1547:1550	arg1	strain					1552:1557	The type strain	1543:1557	The type strain	1543:1557	The type strain is S14-144T (=CCTCC AB 2015345T=KCTC 39796T).
31665101	1	19	theme	Shetland	64:71	arg1	soil					81:84	Antarctica South Shetland Islands soil	47:84	Antarctica South Shetland Islands soil	47:84	nov., isolated from Antarctica South Shetland Islands soil.
31665101	10	20	theme	phylogenetic	1340:1351	arg1	analysis					1384:1391	the phylogenetic, phenotypic and chemotaxonomic analysis	1336:1391	analysis	1384:1391	On the basis of the phylogenetic, phenotypic and chemotaxonomic analysis, strain S14-144T is considered to represent a novel species of the genus Nakamurella, for which the name Nakamurella antarctica sp.
31665101	8	21	dep	C16 	973:976	arg1	ω6c					1000:1002	ω6c	1000:1002	ω6c	1000:1002	The major cellular fatty acids of strain S14-144T were summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c) and C16 : 0.
31665101	8	21	dep	C16 	973:976	arg1	ω7c					981:983	ω7c	981:983	ω7c	981:983	The major cellular fatty acids of strain S14-144T were summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c) and C16 : 0.
31665101	8	21	dep	C16 	973:976	arg1	C16 					992:995	C16 	992:995	C16 	992:995	The major cellular fatty acids of strain S14-144T were summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c) and C16 : 0.
31665101	5	22	theme	rRNA	466:469	arg1	sequences					476:484	16S rRNA gene sequences	462:484	16S rRNA gene sequences	462:484	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain S14-144T formed a lineage within the genus Nakamurella and shared the highest 16S rRNA gene sequence similarity with Nakamurella deserti 12Sc4-1T (96.5 %) and Nakamurella silvestrisS20-107T (96.4 %).
31665101	1	23	theme	Islands	73:79	arg1	soil					81:84	Antarctica South Shetland Islands soil	47:84	Antarctica South Shetland Islands soil	47:84	nov., isolated from Antarctica South Shetland Islands soil.
31665101	10	24	theme	novel	1439:1443	arg1	species					1445:1451	a novel species	1437:1451	a novel species	1437:1451	On the basis of the phylogenetic, phenotypic and chemotaxonomic analysis, strain S14-144T is considered to represent a novel species of the genus Nakamurella, for which the name Nakamurella antarctica sp.
31665101	0	25	theme	antarctica	12:21	arg1	sp					23:24	Nakamurella antarctica sp	0:24	Nakamurella antarctica sp.	0:25	Nakamurella antarctica sp.
31665101	6	26	theme	strain	769:774	arg1	genomes					758:764	the genomes	754:764	the genomes of strain 14-144T and the type strain of the species, N. deserti,	754:830	The average nucleotide identity value between the genomes of strain 14-144T and the type strain of the species, N. deserti, was 72.0 % .
31665101	8	27	theme	cellular	910:917	arg1	acids					925:929	The major cellular fatty acids	900:929	The major cellular fatty acids of strain S14-144T	900:948	The major cellular fatty acids of strain S14-144T were summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c) and C16 : 0.
31665101	5	28	theme	gene	471:474	arg1	sequences					476:484	16S rRNA gene sequences	462:484	16S rRNA gene sequences	462:484	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain S14-144T formed a lineage within the genus Nakamurella and shared the highest 16S rRNA gene sequence similarity with Nakamurella deserti 12Sc4-1T (96.5 %) and Nakamurella silvestrisS20-107T (96.4 %).
31665101	8	29	theme	S14-144T	941:948	arg1	acids					925:929	The major cellular fatty acids	900:929	The major cellular fatty acids of strain S14-144T	900:948	The major cellular fatty acids of strain S14-144T were summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c) and C16 : 0.
31665101	0	30	theme	Nakamurella	0:10	arg1	sp					23:24	Nakamurella antarctica sp	0:24	Nakamurella antarctica sp.	0:25	Nakamurella antarctica sp.
31665101	8	31	theme	major	904:908	arg1	acids					925:929	The major cellular fatty acids	900:929	The major cellular fatty acids of strain S14-144T	900:948	The major cellular fatty acids of strain S14-144T were summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c) and C16 : 0.
31665101	2	32	dep	22	236:237	arg1	59°					247:249	59° 42	247:252	59° 42	247:252	A pale-yellow bacterial strain, designated S14-144T, was isolated from tundra soil sampled near the Antarctic Peninsula, South Shetland Islands (62° 22' 34″ S, 59° 42' 34″ W).
31665101	2	32	dep	22	236:237	arg1	S					244:244	34″ S	240:244	34″ S	240:244	A pale-yellow bacterial strain, designated S14-144T, was isolated from tundra soil sampled near the Antarctic Peninsula, South Shetland Islands (62° 22' 34″ S, 59° 42' 34″ W).
31665101	2	32	dep	22	236:237	arg1	W					259:259	W	259:259	62° 22' 34″ S, 59° 42' 34″ W	232:259	A pale-yellow bacterial strain, designated S14-144T, was isolated from tundra soil sampled near the Antarctic Peninsula, South Shetland Islands (62° 22' 34″ S, 59° 42' 34″ W).
31665101	5	33	theme	gene	595:598	arg1	similarity					609:618	the highest 16S rRNA gene sequence similarity	574:618	the highest 16S rRNA gene sequence similarity	574:618	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain S14-144T formed a lineage within the genus Nakamurella and shared the highest 16S rRNA gene sequence similarity with Nakamurella deserti 12Sc4-1T (96.5 %) and Nakamurella silvestrisS20-107T (96.4 %).
31665101	9	34	from	acid	1283:1286	arg1	peptidoglycan					1305:1317	the cell-wall peptidoglycan	1291:1317	the cell-wall peptidoglycan	1291:1317	The strain contained MK-8(H4) as the predominant respiratory quinone, phosphatidylethanolamine and diphosphatidylglycerol as the major polar lipids, rhamnose, ribose and glucose as the major whole-cell sugars, and meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
31665101	10	35	theme	Nakamurella	1466:1476	arg1	species					1445:1451	a novel species	1437:1451	a novel species	1437:1451	On the basis of the phylogenetic, phenotypic and chemotaxonomic analysis, strain S14-144T is considered to represent a novel species of the genus Nakamurella, for which the name Nakamurella antarctica sp.
31665101	10	36	theme	chemotaxonomic	1369:1382	arg1	analysis					1384:1391	the phylogenetic, phenotypic and chemotaxonomic analysis	1336:1391	analysis	1384:1391	On the basis of the phylogenetic, phenotypic and chemotaxonomic analysis, strain S14-144T is considered to represent a novel species of the genus Nakamurella, for which the name Nakamurella antarctica sp.
31665101	5	37	theme	sequence	600:607	arg1	similarity					609:618	the highest 16S rRNA gene sequence similarity	574:618	the highest 16S rRNA gene sequence similarity	574:618	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain S14-144T formed a lineage within the genus Nakamurella and shared the highest 16S rRNA gene sequence similarity with Nakamurella deserti 12Sc4-1T (96.5 %) and Nakamurella silvestrisS20-107T (96.4 %).
31665101	10	38	theme	genus	1460:1464	arg1	Nakamurella					1466:1476	the genus Nakamurella	1456:1476	the genus Nakamurella	1456:1476	On the basis of the phylogenetic, phenotypic and chemotaxonomic analysis, strain S14-144T is considered to represent a novel species of the genus Nakamurella, for which the name Nakamurella antarctica sp.
31665101	5	39	theme	Nakamurella	667:677	arg1	silvestrisS20-107T					679:696	Nakamurella silvestrisS20-107T	667:696	Nakamurella silvestrisS20-107T (96.4 %)	667:705	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain S14-144T formed a lineage within the genus Nakamurella and shared the highest 16S rRNA gene sequence similarity with Nakamurella deserti 12Sc4-1T (96.5 %) and Nakamurella silvestrisS20-107T (96.4 %).
31665101	5	39	theme	Nakamurella	667:677	arg1	%					704:704	96.4 %	699:704	96.4 %	699:704	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain S14-144T formed a lineage within the genus Nakamurella and shared the highest 16S rRNA gene sequence similarity with Nakamurella deserti 12Sc4-1T (96.5 %) and Nakamurella silvestrisS20-107T (96.4 %).
31665101	6	40	dep	identity	731:738	arg1	value					740:744	value	740:744	The average nucleotide identity value between the genomes of strain 14-144T and the type strain of the species, N. deserti,	708:830	The average nucleotide identity value between the genomes of strain 14-144T and the type strain of the species, N. deserti, was 72.0 % .
31665101	8	41	theme	strain	934:939	arg1	S14-144T					941:948	strain S14-144T	934:948	strain S14-144T	934:948	The major cellular fatty acids of strain S14-144T were summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c) and C16 : 0.
31665101	9	42	theme	meso-diaminopimelic	1232:1250	arg1	acid					1252:1255	meso-diaminopimelic acid	1232:1255	meso-diaminopimelic acid	1232:1255	The strain contained MK-8(H4) as the predominant respiratory quinone, phosphatidylethanolamine and diphosphatidylglycerol as the major polar lipids, rhamnose, ribose and glucose as the major whole-cell sugars, and meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
31665101	2	43	dep	Islands	223:229	arg1	22					236:237	22	236:237	22	236:237	A pale-yellow bacterial strain, designated S14-144T, was isolated from tundra soil sampled near the Antarctic Peninsula, South Shetland Islands (62° 22' 34″ S, 59° 42' 34″ W).
31665101	2	44	theme	pale-yellow	89:99	arg1	strain					111:116	A pale-yellow bacterial strain	87:116	A pale-yellow bacterial strain	87:116	A pale-yellow bacterial strain, designated S14-144T, was isolated from tundra soil sampled near the Antarctic Peninsula, South Shetland Islands (62° 22' 34″ S, 59° 42' 34″ W).
31665101	2	45	theme	tundra	158:163	arg1	soil					165:168	tundra soil	158:168	tundra soil sampled near the Antarctic Peninsula, South Shetland Islands (62° 22' 34″ S, 59° 42' 34″ W)	158:260	A pale-yellow bacterial strain, designated S14-144T, was isolated from tundra soil sampled near the Antarctic Peninsula, South Shetland Islands (62° 22' 34″ S, 59° 42' 34″ W).
31665101	7	46	theme	strain	868:873	arg1	S14-144T					875:882	strain S14-144T	868:882	strain S14-144T	868:882	The DNA G+C content of strain S14-144T was 61.6 mol% .
31665101	9	47	theme	diagnostic	1264:1273	arg1	acid					1283:1286	the diagnostic diamino acid	1260:1286	the diagnostic diamino acid in the cell-wall peptidoglycan	1260:1317	The strain contained MK-8(H4) as the predominant respiratory quinone, phosphatidylethanolamine and diphosphatidylglycerol as the major polar lipids, rhamnose, ribose and glucose as the major whole-cell sugars, and meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
31665101	9	47	theme	diagnostic	1264:1273	arg1	MK-8					1039:1042	MK-8	1039:1042	MK-8(H4)	1039:1046	The strain contained MK-8(H4) as the predominant respiratory quinone, phosphatidylethanolamine and diphosphatidylglycerol as the major polar lipids, rhamnose, ribose and glucose as the major whole-cell sugars, and meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
31665101	4	48	theme	NaCl	425:428	arg1	presence					401:408	the presence	397:408	the presence of 0-5 % (w/v) NaCl	397:428	Growth occurred at 4-28 °C, at pH 5.0-9.0 and in the presence of 0-5 % (w/v) NaCl.
31665101	7	49	theme	mol	893:895	arg1	%					896:896	61.6 mol%	888:896	61.6 mol%	888:896	The DNA G+C content of strain S14-144T was 61.6 mol% .
31665101	7	49	theme	mol	893:895	arg1	content					857:863	The DNA G+C content	845:863	The DNA G+C content of strain S14-144T	845:882	The DNA G+C content of strain S14-144T was 61.6 mol% .
31665101	7	50	theme	S14-144T	875:882	arg1	%					896:896	61.6 mol%	888:896	61.6 mol%	888:896	The DNA G+C content of strain S14-144T was 61.6 mol% .
31665101	7	50	theme	S14-144T	875:882	arg1	content					857:863	The DNA G+C content	845:863	The DNA G+C content of strain S14-144T	845:882	The DNA G+C content of strain S14-144T was 61.6 mol% .
31665101	9	51	theme	diamino	1275:1281	arg1	acid					1283:1286	the diagnostic diamino acid	1260:1286	the diagnostic diamino acid in the cell-wall peptidoglycan	1260:1317	The strain contained MK-8(H4) as the predominant respiratory quinone, phosphatidylethanolamine and diphosphatidylglycerol as the major polar lipids, rhamnose, ribose and glucose as the major whole-cell sugars, and meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
31665101	9	51	theme	diamino	1275:1281	arg1	MK-8					1039:1042	MK-8	1039:1042	MK-8(H4)	1039:1046	The strain contained MK-8(H4) as the predominant respiratory quinone, phosphatidylethanolamine and diphosphatidylglycerol as the major polar lipids, rhamnose, ribose and glucose as the major whole-cell sugars, and meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
31665101	6	52	theme	type	792:795	arg1	strain					797:802	the type strain	788:802	the type strain of the species	788:817	The average nucleotide identity value between the genomes of strain 14-144T and the type strain of the species, N. deserti, was 72.0 % .
31665101	6	52	theme	type	792:795	arg1	deserti					823:829	deserti	823:829	deserti	823:829	The average nucleotide identity value between the genomes of strain 14-144T and the type strain of the species, N. deserti, was 72.0 % .
31665101	4	53	dep	%	417:417	arg1	w/v					420:422	w/v	420:422	w/v	420:422	Growth occurred at 4-28 °C, at pH 5.0-9.0 and in the presence of 0-5 % (w/v) NaCl.
31665101	5	54	theme	highest	578:584	arg1	similarity					609:618	the highest 16S rRNA gene sequence similarity	574:618	the highest 16S rRNA gene sequence similarity	574:618	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain S14-144T formed a lineage within the genus Nakamurella and shared the highest 16S rRNA gene sequence similarity with Nakamurella deserti 12Sc4-1T (96.5 %) and Nakamurella silvestrisS20-107T (96.4 %).
31665101	2	55	attach	isolated	144:151	arg1	soil					165:168	tundra soil	158:168	tundra soil sampled near the Antarctic Peninsula, South Shetland Islands (62° 22' 34″ S, 59° 42' 34″ W)	158:260	A pale-yellow bacterial strain, designated S14-144T, was isolated from tundra soil sampled near the Antarctic Peninsula, South Shetland Islands (62° 22' 34″ S, 59° 42' 34″ W).
31665101	2	55	attach	isolated	144:151	arg2	strain					111:116	A pale-yellow bacterial strain	87:116	A pale-yellow bacterial strain	87:116	A pale-yellow bacterial strain, designated S14-144T, was isolated from tundra soil sampled near the Antarctic Peninsula, South Shetland Islands (62° 22' 34″ S, 59° 42' 34″ W).
31665101	9	56	contain	contained	1029:1037	arg2	MK-8					1039:1042	MK-8	1039:1042	MK-8(H4)	1039:1046	The strain contained MK-8(H4) as the predominant respiratory quinone, phosphatidylethanolamine and diphosphatidylglycerol as the major polar lipids, rhamnose, ribose and glucose as the major whole-cell sugars, and meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
31665101	9	56	contain	contained	1029:1037	arg1	strain					1022:1027	The strain	1018:1027	The strain	1018:1027	The strain contained MK-8(H4) as the predominant respiratory quinone, phosphatidylethanolamine and diphosphatidylglycerol as the major polar lipids, rhamnose, ribose and glucose as the major whole-cell sugars, and meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
31665101	9	56	contain	contained	1029:1037	arg2	H4					1044:1045	H4	1044:1045	H4	1044:1045	The strain contained MK-8(H4) as the predominant respiratory quinone, phosphatidylethanolamine and diphosphatidylglycerol as the major polar lipids, rhamnose, ribose and glucose as the major whole-cell sugars, and meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
31665101	9	56	contain	contained	1029:1037	arg2	quinone					1079:1085	the predominant respiratory quinone	1051:1085	the predominant respiratory quinone	1051:1085	The strain contained MK-8(H4) as the predominant respiratory quinone, phosphatidylethanolamine and diphosphatidylglycerol as the major polar lipids, rhamnose, ribose and glucose as the major whole-cell sugars, and meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
31665101	9	56	contain	contained	1029:1037	arg2	lipids					1159:1164	the major polar lipids	1143:1164	the major polar lipids	1143:1164	The strain contained MK-8(H4) as the predominant respiratory quinone, phosphatidylethanolamine and diphosphatidylglycerol as the major polar lipids, rhamnose, ribose and glucose as the major whole-cell sugars, and meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
31665101	9	56	contain	contained	1029:1037	arg2	acid					1283:1286	the diagnostic diamino acid	1260:1286	the diagnostic diamino acid in the cell-wall peptidoglycan	1260:1317	The strain contained MK-8(H4) as the predominant respiratory quinone, phosphatidylethanolamine and diphosphatidylglycerol as the major polar lipids, rhamnose, ribose and glucose as the major whole-cell sugars, and meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
31665101	6	57	theme	species	811:817	arg1	strain					797:802	the type strain	788:802	the type strain of the species	788:817	The average nucleotide identity value between the genomes of strain 14-144T and the type strain of the species, N. deserti, was 72.0 % .
31665101	6	57	theme	species	811:817	arg1	strain					769:774	strain 14-144T	769:782	strain 14-144T	769:782	The average nucleotide identity value between the genomes of strain 14-144T and the type strain of the species, N. deserti, was 72.0 % .
31665101	6	57	theme	species	811:817	arg1	deserti					823:829	deserti	823:829	deserti	823:829	The average nucleotide identity value between the genomes of strain 14-144T and the type strain of the species, N. deserti, was 72.0 % .
31665101	5	58	theme	16S	586:588	arg1	similarity					609:618	the highest 16S rRNA gene sequence similarity	574:618	the highest 16S rRNA gene sequence similarity	574:618	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain S14-144T formed a lineage within the genus Nakamurella and shared the highest 16S rRNA gene sequence similarity with Nakamurella deserti 12Sc4-1T (96.5 %) and Nakamurella silvestrisS20-107T (96.4 %).
31665101	2	59	theme	Antarctic	187:195	arg1	Islands					223:229	Islands	223:229	Islands	223:229	A pale-yellow bacterial strain, designated S14-144T, was isolated from tundra soil sampled near the Antarctic Peninsula, South Shetland Islands (62° 22' 34″ S, 59° 42' 34″ W).
31665101	2	59	theme	Antarctic	187:195	arg1	Peninsula					197:205	the Antarctic Peninsula	183:205	the Antarctic Peninsula	183:205	A pale-yellow bacterial strain, designated S14-144T, was isolated from tundra soil sampled near the Antarctic Peninsula, South Shetland Islands (62° 22' 34″ S, 59° 42' 34″ W).
31665101	9	60	theme	major	1147:1151	arg1	lipids					1159:1164	the major polar lipids	1143:1164	the major polar lipids	1143:1164	The strain contained MK-8(H4) as the predominant respiratory quinone, phosphatidylethanolamine and diphosphatidylglycerol as the major polar lipids, rhamnose, ribose and glucose as the major whole-cell sugars, and meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
31665101	9	60	theme	major	1147:1151	arg1	MK-8					1039:1042	MK-8	1039:1042	MK-8(H4)	1039:1046	The strain contained MK-8(H4) as the predominant respiratory quinone, phosphatidylethanolamine and diphosphatidylglycerol as the major polar lipids, rhamnose, ribose and glucose as the major whole-cell sugars, and meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
31665101	5	61	theme	16S	462:464	arg1	sequences					476:484	16S rRNA gene sequences	462:484	16S rRNA gene sequences	462:484	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain S14-144T formed a lineage within the genus Nakamurella and shared the highest 16S rRNA gene sequence similarity with Nakamurella deserti 12Sc4-1T (96.5 %) and Nakamurella silvestrisS20-107T (96.4 %).
31665101	5	62	theme	rRNA	590:593	arg1	similarity					609:618	the highest 16S rRNA gene sequence similarity	574:618	the highest 16S rRNA gene sequence similarity	574:618	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain S14-144T formed a lineage within the genus Nakamurella and shared the highest 16S rRNA gene sequence similarity with Nakamurella deserti 12Sc4-1T (96.5 %) and Nakamurella silvestrisS20-107T (96.4 %).
31665101	6	63	theme	nucleotide	720:729	arg1	identity					731:738	The average nucleotide identity	708:738	The average nucleotide identity value between the genomes of strain 14-144T and the type strain of the species, N. deserti,	708:830	The average nucleotide identity value between the genomes of strain 14-144T and the type strain of the species, N. deserti, was 72.0 % .
31665101	6	63	theme	nucleotide	720:729	arg1	%					841:841	72.0 %	836:841	72.0 %	836:841	The average nucleotide identity value between the genomes of strain 14-144T and the type strain of the species, N. deserti, was 72.0 % .
31665101	12	64	theme	2015345T=KCTC	1582:1594	arg1	39796T					1596:1601	=CCTCC AB 2015345T=KCTC 39796T	1572:1601	=CCTCC AB 2015345T=KCTC 39796T	1572:1601	The type strain is S14-144T (=CCTCC AB 2015345T=KCTC 39796T).
31665101	12	64	theme	2015345T=KCTC	1582:1594	arg1	S14-144T					1562:1569	S14-144T	1562:1569	S14-144T (=CCTCC AB 2015345T=KCTC 39796T)	1562:1602	The type strain is S14-144T (=CCTCC AB 2015345T=KCTC 39796T).
31665101	7	65	theme	DNA	849:851	arg1	%					896:896	61.6 mol%	888:896	61.6 mol%	888:896	The DNA G+C content of strain S14-144T was 61.6 mol% .
31665101	7	65	theme	DNA	849:851	arg1	content					857:863	The DNA G+C content	845:863	The DNA G+C content of strain S14-144T	845:882	The DNA G+C content of strain S14-144T was 61.6 mol% .
31665101	10	66	theme	antarctica	1510:1519	arg1	sp					1521:1522	the name Nakamurella antarctica sp	1489:1522	the name Nakamurella antarctica sp	1489:1522	On the basis of the phylogenetic, phenotypic and chemotaxonomic analysis, strain S14-144T is considered to represent a novel species of the genus Nakamurella, for which the name Nakamurella antarctica sp.
31665101	6	67	theme	average	712:718	arg1	identity					731:738	The average nucleotide identity	708:738	The average nucleotide identity value between the genomes of strain 14-144T and the type strain of the species, N. deserti,	708:830	The average nucleotide identity value between the genomes of strain 14-144T and the type strain of the species, N. deserti, was 72.0 % .
31665101	6	67	theme	average	712:718	arg1	%					841:841	72.0 %	836:841	72.0 %	836:841	The average nucleotide identity value between the genomes of strain 14-144T and the type strain of the species, N. deserti, was 72.0 % .
31665101	7	68	theme	G+C	853:855	arg1	%					896:896	61.6 mol%	888:896	61.6 mol%	888:896	The DNA G+C content of strain S14-144T was 61.6 mol% .
31665101	7	68	theme	G+C	853:855	arg1	content					857:863	The DNA G+C content	845:863	The DNA G+C content of strain S14-144T	845:882	The DNA G+C content of strain S14-144T was 61.6 mol% .
31665101	9	69	theme	cell-wall	1295:1303	arg1	peptidoglycan					1305:1317	the cell-wall peptidoglycan	1291:1317	the cell-wall peptidoglycan	1291:1317	The strain contained MK-8(H4) as the predominant respiratory quinone, phosphatidylethanolamine and diphosphatidylglycerol as the major polar lipids, rhamnose, ribose and glucose as the major whole-cell sugars, and meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
31665101	9	70	theme	predominant	1055:1065	arg1	MK-8					1039:1042	MK-8	1039:1042	MK-8(H4)	1039:1046	The strain contained MK-8(H4) as the predominant respiratory quinone, phosphatidylethanolamine and diphosphatidylglycerol as the major polar lipids, rhamnose, ribose and glucose as the major whole-cell sugars, and meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
31665101	9	70	theme	predominant	1055:1065	arg1	quinone					1079:1085	the predominant respiratory quinone	1051:1085	the predominant respiratory quinone	1051:1085	The strain contained MK-8(H4) as the predominant respiratory quinone, phosphatidylethanolamine and diphosphatidylglycerol as the major polar lipids, rhamnose, ribose and glucose as the major whole-cell sugars, and meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
31665101	9	71	theme	major	1203:1207	arg1	sugars					1220:1225	the major whole-cell sugars	1199:1225	the major whole-cell sugars	1199:1225	The strain contained MK-8(H4) as the predominant respiratory quinone, phosphatidylethanolamine and diphosphatidylglycerol as the major polar lipids, rhamnose, ribose and glucose as the major whole-cell sugars, and meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
31665101	4	72	theme	%	417:417	arg1	NaCl					425:428	0-5 % (w/v) NaCl	413:428	0-5 % (w/v) NaCl	413:428	Growth occurred at 4-28 °C, at pH 5.0-9.0 and in the presence of 0-5 % (w/v) NaCl.
31665101	10	73	theme	strain	1394:1399	arg1	S14-144T					1401:1408	strain S14-144T	1394:1408	strain S14-144T	1394:1408	On the basis of the phylogenetic, phenotypic and chemotaxonomic analysis, strain S14-144T is considered to represent a novel species of the genus Nakamurella, for which the name Nakamurella antarctica sp.
31665101	12	74	theme	=CCTCC	1572:1577	arg1	39796T					1596:1601	=CCTCC AB 2015345T=KCTC 39796T	1572:1601	=CCTCC AB 2015345T=KCTC 39796T	1572:1601	The type strain is S14-144T (=CCTCC AB 2015345T=KCTC 39796T).
31665101	12	74	theme	=CCTCC	1572:1577	arg1	S14-144T					1562:1569	S14-144T	1562:1569	S14-144T (=CCTCC AB 2015345T=KCTC 39796T)	1562:1602	The type strain is S14-144T (=CCTCC AB 2015345T=KCTC 39796T).
31665101	8	75	dep	feature	962:968	arg1	C16 					973:976	C16 	973:976	C16 	973:976	The major cellular fatty acids of strain S14-144T were summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c) and C16 : 0.
31665101	10	76	theme	phenotypic	1354:1363	arg1	analysis					1384:1391	the phylogenetic, phenotypic and chemotaxonomic analysis	1336:1391	analysis	1384:1391	On the basis of the phylogenetic, phenotypic and chemotaxonomic analysis, strain S14-144T is considered to represent a novel species of the genus Nakamurella, for which the name Nakamurella antarctica sp.
31761212	3	0	theme	excellent	535:543	arg1	cytocompatibility					545:561	excellent cytocompatibility	535:561	excellent cytocompatibility	535:561	In this study, we have developed fiber-reinforced composite hydrogels that exhibits significantly enhanced mechanical strength, reduced gelling time, and excellent cytocompatibility.
31761212	5	1	theme	chitosan	840:847	arg1	solution					849:856	the chitosan solution	836:856	the chitosan solution	836:856	The effect of βGP concentration on gelation time was studied by varying the concentration of βGP added to the chitosan solution.
31761212	6	2	theme	physiological	987:999	arg1	pH					1001:1002	physiological pH	987:1002	physiological pH	987:1002	Various combinations were tested to create a suitable hydrogel environment for cell encapsulation, growth, and proliferation at physiological pH and temperature.
31761212	1	3	theme	fast	167:170	arg1	gelation					172:179	fast gelation	167:179	fast gelation	167:179	The use of injectable hydrogels is currently restricted by the challenge of achieving fast gelation, good mechanical strength, and cytocompatibility.
31761212	4	4	theme	hydroxyapatite	643:656	arg1	PEC					687:689	PEC	687:689	PEC	687:689	The practicability of developing a chitosan-based thermogelling solution using hydroxyapatite and polyelectrolyte complex (PEC) self-assembled fibers were evaluated.
31761212	4	4	theme	hydroxyapatite	643:656	arg1	fibers					707:712	hydroxyapatite and polyelectrolyte complex (PEC) self-assembled fibers	643:712	hydroxyapatite and polyelectrolyte complex (PEC) self-assembled fibers	643:712	The practicability of developing a chitosan-based thermogelling solution using hydroxyapatite and polyelectrolyte complex (PEC) self-assembled fibers were evaluated.
31761212	8	5	theme	physiological	1237:1249	arg1	temperature					1255:1265	temperature	1255:1265	temperature	1255:1265	The gelation time was reduced to 3 min, and the hydrogels had porous structures and gels at physiological pH, temperature, and showed >80% viability for MTT assay to MG63 cells.
31761212	8	5	theme	physiological	1237:1249	arg1	pH					1251:1252	physiological pH	1237:1252	physiological pH	1237:1252	The gelation time was reduced to 3 min, and the hydrogels had porous structures and gels at physiological pH, temperature, and showed >80% viability for MTT assay to MG63 cells.
31761212	9	6	theme	fiber	1357:1361	arg1	hydrogels					1374:1382	PEC fiber reinforced hydrogels	1353:1382	PEC fiber reinforced hydrogels	1353:1382	Moreover, confocal imaging of PEC fiber reinforced hydrogels showed noticeable viability and proliferation.
31761212	12	7	from	applications	1783:1794	arg1	helpful					1753:1759	helpful	1753:1759	helpful	1753:1759	The combination of hydroxyapatite and polymer self-assembly technique improved the efficiency of injectable hydrogels that are helpful in minimally invasive applications.
31761212	4	8	theme	complex	678:684	arg1	PEC					687:689	PEC	687:689	PEC	687:689	The practicability of developing a chitosan-based thermogelling solution using hydroxyapatite and polyelectrolyte complex (PEC) self-assembled fibers were evaluated.
31761212	4	8	theme	complex	678:684	arg1	fibers					707:712	hydroxyapatite and polyelectrolyte complex (PEC) self-assembled fibers	643:712	hydroxyapatite and polyelectrolyte complex (PEC) self-assembled fibers	643:712	The practicability of developing a chitosan-based thermogelling solution using hydroxyapatite and polyelectrolyte complex (PEC) self-assembled fibers were evaluated.
31761212	9	9	theme	reinforced	1363:1372	arg1	hydrogels					1374:1382	PEC fiber reinforced hydrogels	1353:1382	PEC fiber reinforced hydrogels	1353:1382	Moreover, confocal imaging of PEC fiber reinforced hydrogels showed noticeable viability and proliferation.
31761212	5	10	theme	βGP	744:746	arg1	concentration					748:760	βGP concentration	744:760	βGP concentration	744:760	The effect of βGP concentration on gelation time was studied by varying the concentration of βGP added to the chitosan solution.
31761212	7	11	theme	Young	1038:1042	arg1	modulus					1044:1050	Young modulus	1038:1050	Young modulus	1038:1050	Determination of Young modulus revealed that PEC fibers reinforced hydrogel was three times stiffer than chitosan-βGP gels.
31761212	4	12	theme	polyelectrolyte	662:676	arg1	complex					678:684	polyelectrolyte complex	662:684	polyelectrolyte complex	662:684	The practicability of developing a chitosan-based thermogelling solution using hydroxyapatite and polyelectrolyte complex (PEC) self-assembled fibers were evaluated.
31761212	9	13	theme	hydrogels	1374:1382	arg1	imaging					1342:1348	confocal imaging	1333:1348	confocal imaging of PEC fiber reinforced hydrogels	1333:1382	Moreover, confocal imaging of PEC fiber reinforced hydrogels showed noticeable viability and proliferation.
31761212	8	14	theme	gelation	1149:1156	arg1	time					1158:1161	The gelation time	1145:1161	The gelation time	1145:1161	The gelation time was reduced to 3 min, and the hydrogels had porous structures and gels at physiological pH, temperature, and showed >80% viability for MTT assay to MG63 cells.
31761212	7	15	theme	modulus	1044:1050	arg1	Determination					1021:1033	Determination	1021:1033	Determination of Young modulus	1021:1050	Determination of Young modulus revealed that PEC fibers reinforced hydrogel was three times stiffer than chitosan-βGP gels.
31761212	2	16	theme	functional	287:296	arg1	materials					298:306	functional materials	287:306	functional materials that combine multiple characteristics and can react to external factors	287:378	Polymeric self-assembly is a potent tool for generating functional materials that combine multiple characteristics and can react to external factors.
31761212	10	17	theme	molecular	1435:1443	arg1	interactions					1445:1456	The molecular interactions	1431:1456	The molecular interactions between gelling agents, polyelectrolytes, and hydroxyapatite	1431:1517	The molecular interactions between gelling agents, polyelectrolytes, and hydroxyapatite were studied using FTIR.
31761212	0	18	theme	bone	56:59	arg1	engineering					68:78	bone tissue engineering	56:78	bone tissue engineering	56:78	Injectable and thermosensitive nanofibrous hydrogel for bone tissue engineering.
31761212	11	19	with	fibers	1592:1597	arg1	NaHCO3					1609:1614	NaHCO3	1609:1614	NaHCO3	1609:1614	We investigated interfacial bonding between PEC fibers with βGP, NaHCO3, and HAp.
31761212	11	19	with	fibers	1592:1597	arg1	HAp					1621:1623	HAp	1621:1623	HAp	1621:1623	We investigated interfacial bonding between PEC fibers with βGP, NaHCO3, and HAp.
31761212	11	19	with	fibers	1592:1597	arg1	βGP					1604:1606	βGP	1604:1606	βGP	1604:1606	We investigated interfacial bonding between PEC fibers with βGP, NaHCO3, and HAp.
31761212	8	20	theme	%	1282:1282	arg1	viability					1284:1292	>80% viability	1279:1292	>80% viability for MTT assay	1279:1306	The gelation time was reduced to 3 min, and the hydrogels had porous structures and gels at physiological pH, temperature, and showed >80% viability for MTT assay to MG63 cells.
31761212	3	21	theme	enhanced	479:486	arg1	strength					499:506	significantly enhanced mechanical strength	465:506	significantly enhanced mechanical strength	465:506	In this study, we have developed fiber-reinforced composite hydrogels that exhibits significantly enhanced mechanical strength, reduced gelling time, and excellent cytocompatibility.
31761212	9	22	theme	noticeable	1391:1400	arg1	viability					1402:1410	noticeable viability	1391:1410	noticeable viability	1391:1410	Moreover, confocal imaging of PEC fiber reinforced hydrogels showed noticeable viability and proliferation.
31761212	6	23	theme	Various	859:865	arg1	combinations					867:878	Various combinations	859:878	Various combinations	859:878	Various combinations were tested to create a suitable hydrogel environment for cell encapsulation, growth, and proliferation at physiological pH and temperature.
31761212	12	24	from	helpful	1753:1759	arg1	applications					1783:1794	minimally invasive applications	1764:1794	minimally invasive applications	1764:1794	The combination of hydroxyapatite and polymer self-assembly technique improved the efficiency of injectable hydrogels that are helpful in minimally invasive applications.
31761212	12	25	theme	invasive	1774:1781	arg1	applications					1783:1794	minimally invasive applications	1764:1794	minimally invasive applications	1764:1794	The combination of hydroxyapatite and polymer self-assembly technique improved the efficiency of injectable hydrogels that are helpful in minimally invasive applications.
31761212	1	26	theme	good	182:185	arg1	strength					198:205	good mechanical strength	182:205	good mechanical strength	182:205	The use of injectable hydrogels is currently restricted by the challenge of achieving fast gelation, good mechanical strength, and cytocompatibility.
31761212	0	27	theme	Injectable	0:9	arg1	nanofibrous					31:41	Injectable and thermosensitive nanofibrous	0:41	Injectable and thermosensitive nanofibrous	0:41	Injectable and thermosensitive nanofibrous hydrogel for bone tissue engineering.
31761212	4	28	theme	thermogelling	614:626	arg1	solution					628:635	a chitosan-based thermogelling solution	597:635	a chitosan-based thermogelling solution using hydroxyapatite and polyelectrolyte complex (PEC) self-assembled fibers	597:712	The practicability of developing a chitosan-based thermogelling solution using hydroxyapatite and polyelectrolyte complex (PEC) self-assembled fibers were evaluated.
31761212	1	29	theme	injectable	92:101	arg1	hydrogels					103:111	injectable hydrogels	92:111	injectable hydrogels	92:111	The use of injectable hydrogels is currently restricted by the challenge of achieving fast gelation, good mechanical strength, and cytocompatibility.
31761212	11	30	theme	interfacial	1560:1570	arg1	bonding					1572:1578	interfacial bonding	1560:1578	interfacial bonding between PEC fibers with βGP, NaHCO3, and HAp	1560:1623	We investigated interfacial bonding between PEC fibers with βGP, NaHCO3, and HAp.
31761212	1	31	theme	mechanical	187:196	arg1	strength					198:205	good mechanical strength	182:205	good mechanical strength	182:205	The use of injectable hydrogels is currently restricted by the challenge of achieving fast gelation, good mechanical strength, and cytocompatibility.
31761212	4	32	theme	chitosan-based	599:612	arg1	solution					628:635	a chitosan-based thermogelling solution	597:635	a chitosan-based thermogelling solution using hydroxyapatite and polyelectrolyte complex (PEC) self-assembled fibers	597:712	The practicability of developing a chitosan-based thermogelling solution using hydroxyapatite and polyelectrolyte complex (PEC) self-assembled fibers were evaluated.
31761212	12	33	theme	self-assembly	1672:1684	arg1	technique					1686:1694	polymer self-assembly technique	1664:1694	polymer self-assembly technique	1664:1694	The combination of hydroxyapatite and polymer self-assembly technique improved the efficiency of injectable hydrogels that are helpful in minimally invasive applications.
31761212	10	34	theme	gelling	1466:1472	arg1	agents					1474:1479	gelling agents	1466:1479	gelling agents	1466:1479	The molecular interactions between gelling agents, polyelectrolytes, and hydroxyapatite were studied using FTIR.
31761212	2	35	theme	multiple	321:328	arg1	characteristics					330:344	multiple characteristics	321:344	multiple characteristics	321:344	Polymeric self-assembly is a potent tool for generating functional materials that combine multiple characteristics and can react to external factors.
31761212	12	36	theme	hydrogels	1734:1742	arg1	efficiency					1709:1718	the efficiency	1705:1718	the efficiency of injectable hydrogels that are helpful in minimally invasive applications	1705:1794	The combination of hydroxyapatite and polymer self-assembly technique improved the efficiency of injectable hydrogels that are helpful in minimally invasive applications.
31761212	0	37	theme	thermosensitive	15:29	arg1	nanofibrous					31:41	Injectable and thermosensitive nanofibrous	0:41	Injectable and thermosensitive nanofibrous	0:41	Injectable and thermosensitive nanofibrous hydrogel for bone tissue engineering.
31761212	12	38	theme	injectable	1723:1732	arg1	hydrogels					1734:1742	injectable hydrogels	1723:1742	injectable hydrogels that are helpful in minimally invasive applications	1723:1794	The combination of hydroxyapatite and polymer self-assembly technique improved the efficiency of injectable hydrogels that are helpful in minimally invasive applications.
31761212	3	39	theme	reduced	509:515	arg1	time					525:528	reduced gelling time	509:528	reduced gelling time	509:528	In this study, we have developed fiber-reinforced composite hydrogels that exhibits significantly enhanced mechanical strength, reduced gelling time, and excellent cytocompatibility.
31761212	3	40	theme	composite	431:439	arg1	hydrogels					441:449	fiber-reinforced composite hydrogels	414:449	fiber-reinforced composite hydrogels that exhibits significantly enhanced mechanical strength, reduced gelling time, and excellent cytocompatibility	414:561	In this study, we have developed fiber-reinforced composite hydrogels that exhibits significantly enhanced mechanical strength, reduced gelling time, and excellent cytocompatibility.
31761212	11	41	theme	PEC	1588:1590	arg1	fibers					1592:1597	PEC fibers	1588:1597	PEC fibers with βGP, NaHCO3, and HAp	1588:1623	We investigated interfacial bonding between PEC fibers with βGP, NaHCO3, and HAp.
31761212	3	42	theme	mechanical	488:497	arg1	strength					499:506	significantly enhanced mechanical strength	465:506	significantly enhanced mechanical strength	465:506	In this study, we have developed fiber-reinforced composite hydrogels that exhibits significantly enhanced mechanical strength, reduced gelling time, and excellent cytocompatibility.
31761212	8	43	theme	>80	1279:1281	arg1	%					1282:1282	%	1282:1282	%	1282:1282	The gelation time was reduced to 3 min, and the hydrogels had porous structures and gels at physiological pH, temperature, and showed >80% viability for MTT assay to MG63 cells.
31761212	6	44	dep	cell	938:941	arg1	encapsulation					943:955	encapsulation	943:955	encapsulation	943:955	Various combinations were tested to create a suitable hydrogel environment for cell encapsulation, growth, and proliferation at physiological pH and temperature.
31761212	5	45	theme	βGP	823:825	arg1	concentration					806:818	the concentration	802:818	the concentration of βGP added to the chitosan solution	802:856	The effect of βGP concentration on gelation time was studied by varying the concentration of βGP added to the chitosan solution.
31761212	0	46	theme	tissue	61:66	arg1	engineering					68:78	bone tissue engineering	56:78	bone tissue engineering	56:78	Injectable and thermosensitive nanofibrous hydrogel for bone tissue engineering.
31761212	12	47	theme	polymer	1664:1670	arg1	technique					1686:1694	polymer self-assembly technique	1664:1694	polymer self-assembly technique	1664:1694	The combination of hydroxyapatite and polymer self-assembly technique improved the efficiency of injectable hydrogels that are helpful in minimally invasive applications.
31761212	5	48	theme	concentration	748:760	arg1	effect					734:739	The effect	730:739	The effect of βGP concentration on gelation time	730:777	The effect of βGP concentration on gelation time was studied by varying the concentration of βGP added to the chitosan solution.
31761212	2	49	theme	Polymeric	231:239	arg1	self-assembly					241:253	Polymeric self-assembly	231:253	Polymeric self-assembly	231:253	Polymeric self-assembly is a potent tool for generating functional materials that combine multiple characteristics and can react to external factors.
31761212	2	49	theme	Polymeric	231:239	arg1	tool					267:270	a potent tool	258:270	a potent tool for generating functional materials that combine multiple characteristics and can react to external factors	258:378	Polymeric self-assembly is a potent tool for generating functional materials that combine multiple characteristics and can react to external factors.
31761212	7	50	theme	stiffer	1113:1119	arg1	times					1107:1111	three times	1101:1111	three times stiffer than chitosan-βGP gels	1101:1142	Determination of Young modulus revealed that PEC fibers reinforced hydrogel was three times stiffer than chitosan-βGP gels.
31761212	2	51	theme	external	363:370	arg1	factors					372:378	external factors	363:378	external factors	363:378	Polymeric self-assembly is a potent tool for generating functional materials that combine multiple characteristics and can react to external factors.
31761212	3	52	theme	fiber-reinforced	414:429	arg1	hydrogels					441:449	fiber-reinforced composite hydrogels	414:449	fiber-reinforced composite hydrogels that exhibits significantly enhanced mechanical strength, reduced gelling time, and excellent cytocompatibility	414:561	In this study, we have developed fiber-reinforced composite hydrogels that exhibits significantly enhanced mechanical strength, reduced gelling time, and excellent cytocompatibility.
31761212	8	53	contain	had	1203:1205	arg2	gels					1229:1232	gels	1229:1232	gels	1229:1232	The gelation time was reduced to 3 min, and the hydrogels had porous structures and gels at physiological pH, temperature, and showed >80% viability for MTT assay to MG63 cells.
31761212	8	53	contain	had	1203:1205	arg1	hydrogels					1193:1201	the hydrogels	1189:1201	the hydrogels	1189:1201	The gelation time was reduced to 3 min, and the hydrogels had porous structures and gels at physiological pH, temperature, and showed >80% viability for MTT assay to MG63 cells.
31761212	8	53	contain	had	1203:1205	arg2	structures					1214:1223	porous structures	1207:1223	porous structures	1207:1223	The gelation time was reduced to 3 min, and the hydrogels had porous structures and gels at physiological pH, temperature, and showed >80% viability for MTT assay to MG63 cells.
31761212	5	54	from	effect	734:739	arg1	time					774:777	gelation time	765:777	gelation time	765:777	The effect of βGP concentration on gelation time was studied by varying the concentration of βGP added to the chitosan solution.
31761212	5	55	theme	gelation	765:772	arg1	time					774:777	gelation time	765:777	gelation time	765:777	The effect of βGP concentration on gelation time was studied by varying the concentration of βGP added to the chitosan solution.
31761212	6	56	theme	hydrogel	913:920	arg1	environment					922:932	a suitable hydrogel environment	902:932	a suitable hydrogel environment for cell encapsulation, growth, and proliferation	902:982	Various combinations were tested to create a suitable hydrogel environment for cell encapsulation, growth, and proliferation at physiological pH and temperature.
31761212	3	57	theme	gelling	517:523	arg1	time					525:528	reduced gelling time	509:528	reduced gelling time	509:528	In this study, we have developed fiber-reinforced composite hydrogels that exhibits significantly enhanced mechanical strength, reduced gelling time, and excellent cytocompatibility.
31761212	1	58	theme	hydrogels	103:111	arg1	use					85:87	The use	81:87	The use of injectable hydrogels	81:111	The use of injectable hydrogels is currently restricted by the challenge of achieving fast gelation, good mechanical strength, and cytocompatibility.
31761212	7	59	theme	chitosan-βGP	1126:1137	arg1	gels					1139:1142	chitosan-βGP gels	1126:1142	chitosan-βGP gels	1126:1142	Determination of Young modulus revealed that PEC fibers reinforced hydrogel was three times stiffer than chitosan-βGP gels.
31761212	6	60	theme	suitable	904:911	arg1	environment					922:932	a suitable hydrogel environment	902:932	a suitable hydrogel environment for cell encapsulation, growth, and proliferation	902:982	Various combinations were tested to create a suitable hydrogel environment for cell encapsulation, growth, and proliferation at physiological pH and temperature.
31761212	12	61	theme	technique	1686:1694	arg1	combination					1630:1640	The combination	1626:1640	The combination of hydroxyapatite and polymer self-assembly technique	1626:1694	The combination of hydroxyapatite and polymer self-assembly technique improved the efficiency of injectable hydrogels that are helpful in minimally invasive applications.
31761212	9	62	theme	confocal	1333:1340	arg1	imaging					1342:1348	confocal imaging	1333:1348	confocal imaging of PEC fiber reinforced hydrogels	1333:1382	Moreover, confocal imaging of PEC fiber reinforced hydrogels showed noticeable viability and proliferation.
31761212	2	63	theme	potent	260:265	arg1	self-assembly					241:253	Polymeric self-assembly	231:253	Polymeric self-assembly	231:253	Polymeric self-assembly is a potent tool for generating functional materials that combine multiple characteristics and can react to external factors.
31761212	2	63	theme	potent	260:265	arg1	tool					267:270	a potent tool	258:270	a potent tool for generating functional materials that combine multiple characteristics and can react to external factors	258:378	Polymeric self-assembly is a potent tool for generating functional materials that combine multiple characteristics and can react to external factors.
31761212	8	64	theme	MTT	1298:1300	arg1	assay					1302:1306	MTT assay	1298:1306	MTT assay	1298:1306	The gelation time was reduced to 3 min, and the hydrogels had porous structures and gels at physiological pH, temperature, and showed >80% viability for MTT assay to MG63 cells.
31761212	4	65	theme	self-assembled	692:705	arg1	PEC					687:689	PEC	687:689	PEC	687:689	The practicability of developing a chitosan-based thermogelling solution using hydroxyapatite and polyelectrolyte complex (PEC) self-assembled fibers were evaluated.
31761212	4	65	theme	self-assembled	692:705	arg1	fibers					707:712	hydroxyapatite and polyelectrolyte complex (PEC) self-assembled fibers	643:712	hydroxyapatite and polyelectrolyte complex (PEC) self-assembled fibers	643:712	The practicability of developing a chitosan-based thermogelling solution using hydroxyapatite and polyelectrolyte complex (PEC) self-assembled fibers were evaluated.
31761212	8	66	theme	porous	1207:1212	arg1	structures					1214:1223	porous structures	1207:1223	porous structures	1207:1223	The gelation time was reduced to 3 min, and the hydrogels had porous structures and gels at physiological pH, temperature, and showed >80% viability for MTT assay to MG63 cells.
31761212	9	67	theme	PEC	1353:1355	arg1	hydrogels					1374:1382	PEC fiber reinforced hydrogels	1353:1382	PEC fiber reinforced hydrogels	1353:1382	Moreover, confocal imaging of PEC fiber reinforced hydrogels showed noticeable viability and proliferation.
31761212	7	68	theme	PEC	1066:1068	arg1	fibers					1070:1075	PEC fibers	1066:1075	PEC fibers	1066:1075	Determination of Young modulus revealed that PEC fibers reinforced hydrogel was three times stiffer than chitosan-βGP gels.
31761212	12	69	theme	hydroxyapatite	1645:1658	arg1	combination					1630:1640	The combination	1626:1640	The combination of hydroxyapatite and polymer self-assembly technique	1626:1694	The combination of hydroxyapatite and polymer self-assembly technique improved the efficiency of injectable hydrogels that are helpful in minimally invasive applications.
31864749	14	0	theme	lower-fat	2346:2354	arg1	types					2363:2367	lower-fat cheese types	2346:2367	lower-fat cheese types	2346:2367	In addition, LS could be used to help soften texture and increase meltability, if desired in lower-fat cheese types.
31864749	1	1	theme	poor	234:237	arg1	attributes					259:268	poor flavor and textural attributes	234:268	poor flavor and textural attributes	234:268	Control of acidity is critical for cheese quality, as high acidity can be associated with poor flavor and textural attributes.
31864749	4	2	theme	cheese	811:816	arg1	acidity					818:824	cheese acidity	811:824	cheese acidity	811:824	Direct standardization of the lactose content of milk during the ultrafiltration process could be a simpler and more effective technique to control cheese acidity.
31864749	13	3	theme	ratio	2150:2154	arg1	standardization					2122:2136	standardization	2122:2136	standardization of the L:CN ratio of milk	2122:2162	These results suggest that standardization of the L:CN ratio of milk could be a useful alternative to WD (or a curd rinse step) to reduce acidity in cheeses.
31864749	13	3	theme	ratio	2150:2154	arg1	alternative					2182:2192	a useful alternative	2173:2192	a useful alternative to WD (or a curd rinse step) to reduce acidity in cheeses	2173:2250	These results suggest that standardization of the L:CN ratio of milk could be a useful alternative to WD (or a curd rinse step) to reduce acidity in cheeses.
31864749	6	4	theme	cheeses	1148:1154	arg1	composition					1063:1073	composition	1063:1073	composition	1063:1073	The composition, texture, functionality, and sensory properties of these LF and RF Gouda cheeses were evaluated.
31864749	6	4	theme	cheeses	1148:1154	arg1	texture					1076:1082	texture	1076:1082	texture	1076:1082	The composition, texture, functionality, and sensory properties of these LF and RF Gouda cheeses were evaluated.
31864749	6	4	theme	cheeses	1148:1154	arg1	functionality					1085:1097	functionality	1085:1097	functionality	1085:1097	The composition, texture, functionality, and sensory properties of these LF and RF Gouda cheeses were evaluated.
31864749	6	4	theme	cheeses	1148:1154	arg1	properties					1112:1121	sensory properties	1104:1121	sensory properties	1104:1121	The composition, texture, functionality, and sensory properties of these LF and RF Gouda cheeses were evaluated.
31864749	3	5	theme	Gouda	509:513	arg1	manufacture					522:532	traditional Gouda cheese manufacture	497:532	traditional Gouda cheese manufacture	497:532	In traditional Gouda cheese manufacture, a critical technique to control acidity is whey dilution (WD); that is, partial removal of whey and its replacement with water.
31864749	6	6	theme	RF	1139:1140	arg1	cheeses					1148:1154	these LF and RF Gouda cheeses	1126:1154	cheeses	1148:1154	The composition, texture, functionality, and sensory properties of these LF and RF Gouda cheeses were evaluated.
31864749	4	7	theme	effective	780:788	arg1	standardization					670:684	Direct standardization	663:684	Direct standardization of the lactose content of milk during the ultrafiltration process	663:750	Direct standardization of the lactose content of milk during the ultrafiltration process could be a simpler and more effective technique to control cheese acidity.
31864749	4	7	theme	effective	780:788	arg1	technique					790:798	a simpler and more effective technique	761:798	a simpler and more effective technique to control cheese acidity	761:824	Direct standardization of the lactose content of milk during the ultrafiltration process could be a simpler and more effective technique to control cheese acidity.
31864749	6	8	theme	LF	1132:1133	arg1	composition					1063:1073	composition	1063:1073	composition	1063:1073	The composition, texture, functionality, and sensory properties of these LF and RF Gouda cheeses were evaluated.
31864749	6	8	theme	LF	1132:1133	arg1	texture					1076:1082	texture	1076:1082	texture	1076:1082	The composition, texture, functionality, and sensory properties of these LF and RF Gouda cheeses were evaluated.
31864749	6	8	theme	LF	1132:1133	arg1	functionality					1085:1097	functionality	1085:1097	functionality	1085:1097	The composition, texture, functionality, and sensory properties of these LF and RF Gouda cheeses were evaluated.
31864749	6	8	theme	LF	1132:1133	arg1	properties					1112:1121	sensory properties	1104:1121	sensory properties	1104:1121	The composition, texture, functionality, and sensory properties of these LF and RF Gouda cheeses were evaluated.
31864749	2	9	theme	alternative	290:300	arg1	method					302:307	an alternative method	287:307	an alternative method to control cheese acidity, specifically in low-fat (LF) and reduced-fat (RF) milled curd, direct-salted Gouda cheese, which involved altering the initial lactose content of cheesemilk	287:491	We investigated an alternative method to control cheese acidity, specifically in low-fat (LF) and reduced-fat (RF) milled curd, direct-salted Gouda cheese, which involved altering the initial lactose content of cheesemilk.
31864749	1	10	theme	cheese	179:184	arg1	quality					186:192	cheese quality	179:192	cheese quality	179:192	Control of acidity is critical for cheese quality, as high acidity can be associated with poor flavor and textural attributes.
31864749	13	11	from	acidity	2233:2239	arg1	cheeses					2244:2250	cheeses	2244:2250	cheeses	2244:2250	These results suggest that standardization of the L:CN ratio of milk could be a useful alternative to WD (or a curd rinse step) to reduce acidity in cheeses.
31864749	12	12	contain	had	2057:2059	arg2	lower					2061:2065	lower	2061:2065	lower	2061:2065	Sensory analysis also indicated that LS cheeses had lower acidity and softer texture.
31864749	12	12	contain	had	2057:2059	arg1	cheeses					2049:2055	LS cheeses	2046:2055	LS cheeses	2046:2055	Sensory analysis also indicated that LS cheeses had lower acidity and softer texture.
31864749	6	13	dep	composition	1063:1073	arg1	The					1059:1061	The	1059:1061	The	1059:1061	The composition, texture, functionality, and sensory properties of these LF and RF Gouda cheeses were evaluated.
31864749	13	14	theme	curd	2206:2209	arg1	WD					2197:2198	WD	2197:2198	WD (or a curd rinse step)	2197:2221	These results suggest that standardization of the L:CN ratio of milk could be a useful alternative to WD (or a curd rinse step) to reduce acidity in cheeses.
31864749	13	14	theme	curd	2206:2209	arg1	step					2217:2220	a curd rinse step	2204:2220	a curd rinse step	2204:2220	These results suggest that standardization of the L:CN ratio of milk could be a useful alternative to WD (or a curd rinse step) to reduce acidity in cheeses.
31864749	10	15	with	cheeses	1769:1775	arg1	temperature					1800:1810	a lower crossover temperature	1782:1810	a lower crossover temperature (melting point) than the cheeses made with WD	1782:1856	Dynamic small-amplitude oscillatory rheology indicated that use of LS led to cheeses with a lower crossover temperature (melting point) than the cheeses made with WD.
31864749	10	15	with	cheeses	1769:1775	arg1	point					1821:1825	melting point	1813:1825	melting point	1813:1825	Dynamic small-amplitude oscillatory rheology indicated that use of LS led to cheeses with a lower crossover temperature (melting point) than the cheeses made with WD.
31864749	0	16	theme	dilution	123:130	arg1	techniques					132:141	whey dilution techniques	118:141	whey dilution techniques	118:141	Low- and reduced-fat milled curd, direct-salted Gouda cheese: Comparison of lactose standardization of cheesemilk and whey dilution techniques.
31864749	5	17	theme	adjustment	960:969	arg1	approach					948:955	the alternative approach	932:955	the alternative approach of adjustment of the lactose content of milk using low-concentration-factor ultrafiltration (LCF-UF)	932:1056	This study compared the effect of traditional WD at 2 different levels, 15 and 30% (WD15 and WD30), with the alternative approach of adjustment of the lactose content of milk using low-concentration-factor ultrafiltration (LCF-UF).
31864749	3	18	theme	control	559:565	arg1	acidity					567:573	control acidity	559:573	control acidity	559:573	In traditional Gouda cheese manufacture, a critical technique to control acidity is whey dilution (WD); that is, partial removal of whey and its replacement with water.
31864749	1	19	theme	high	198:201	arg1	acidity					203:209	high acidity	198:209	high acidity	198:209	Control of acidity is critical for cheese quality, as high acidity can be associated with poor flavor and textural attributes.
31864749	8	20	theme	1.8	1323:1325	arg1	ratio					1300:1304	a lactose-to-casein (L:CN) ratio	1273:1304	a lactose-to-casein (L:CN) ratio of approximately 1.8, which is the typical ratio found in milk	1273:1367	Milks used for cheesemaking had a lactose-to-casein (L:CN) ratio of approximately 1.8, which is the typical ratio found in milk, whereas milks prepared with lactose standardization (LS) were made from UF concentrated milks with water added during filtration to achieve a L:CN ratio of approximately 1.1.
31864749	10	21	theme	crossover	1790:1798	arg1	temperature					1800:1810	a lower crossover temperature	1782:1810	a lower crossover temperature (melting point) than the cheeses made with WD	1782:1856	Dynamic small-amplitude oscillatory rheology indicated that use of LS led to cheeses with a lower crossover temperature (melting point) than the cheeses made with WD.
31864749	10	21	theme	crossover	1790:1798	arg1	point					1821:1825	melting point	1813:1825	melting point	1813:1825	Dynamic small-amplitude oscillatory rheology indicated that use of LS led to cheeses with a lower crossover temperature (melting point) than the cheeses made with WD.
31864749	11	22	theme	insoluble	1890:1898	arg1	contents					1903:1910	lower insoluble Ca contents	1884:1910	lower insoluble Ca contents	1884:1910	Cheeses made with LS had lower insoluble Ca contents, likely caused by the addition of water required to achieve the lower L:CN ratio in these milks.
31864749	8	23	with	milks	1458:1462	arg1	water					1469:1473	water	1469:1473	water added during filtration	1469:1497	Milks used for cheesemaking had a lactose-to-casein (L:CN) ratio of approximately 1.8, which is the typical ratio found in milk, whereas milks prepared with lactose standardization (LS) were made from UF concentrated milks with water added during filtration to achieve a L:CN ratio of approximately 1.1.
31864749	2	24	theme	initial	455:461	arg1	content					471:477	the initial lactose content	451:477	the initial lactose content of cheesemilk	451:491	We investigated an alternative method to control cheese acidity, specifically in low-fat (LF) and reduced-fat (RF) milled curd, direct-salted Gouda cheese, which involved altering the initial lactose content of cheesemilk.
31864749	8	25	theme	CN	1514:1515	arg1	ratio					1517:1521	a L:CN ratio	1510:1521	a L:CN ratio of approximately 1.1	1510:1542	Milks used for cheesemaking had a lactose-to-casein (L:CN) ratio of approximately 1.8, which is the typical ratio found in milk, whereas milks prepared with lactose standardization (LS) were made from UF concentrated milks with water added during filtration to achieve a L:CN ratio of approximately 1.1.
31864749	7	26	used	used	1235:1238	arg2	protocol					1222:1229	A milled curd, direct-salted cheese manufacturing protocol	1172:1229	A milled curd, direct-salted cheese manufacturing protocol	1172:1229	A milled curd, direct-salted cheese manufacturing protocol was used.
31864749	5	27	theme	milk	997:1000	arg1	content					986:992	the lactose content	974:992	the lactose content of milk using low-concentration-factor ultrafiltration (LCF-UF)	974:1056	This study compared the effect of traditional WD at 2 different levels, 15 and 30% (WD15 and WD30), with the alternative approach of adjustment of the lactose content of milk using low-concentration-factor ultrafiltration (LCF-UF).
31864749	9	28	theme	lactose	1582:1588	arg1	contents					1606:1613	lower lactose and lactic acid contents	1576:1613	lower lactose and lactic acid contents	1576:1613	Cheeses made with LS exhibited lower lactose and lactic acid contents than WD30 and WD15, leading to significantly higher pH values in the cheese.
31864749	5	29	theme	low-concentration-factor	1008:1031	arg1	LCF-UF					1050:1055	LCF-UF	1050:1055	LCF-UF	1050:1055	This study compared the effect of traditional WD at 2 different levels, 15 and 30% (WD15 and WD30), with the alternative approach of adjustment of the lactose content of milk using low-concentration-factor ultrafiltration (LCF-UF).
31864749	5	29	theme	low-concentration-factor	1008:1031	arg1	ultrafiltration					1033:1047	low-concentration-factor ultrafiltration	1008:1047	low-concentration-factor ultrafiltration (LCF-UF)	1008:1056	This study compared the effect of traditional WD at 2 different levels, 15 and 30% (WD15 and WD30), with the alternative approach of adjustment of the lactose content of milk using low-concentration-factor ultrafiltration (LCF-UF).
31864749	12	30	theme	softer	2079:2084	arg1	texture					2086:2092	softer texture	2079:2092	softer texture	2079:2092	Sensory analysis also indicated that LS cheeses had lower acidity and softer texture.
31864749	1	31	theme	acidity	155:161	arg1	Control					144:150	Control	144:150	Control of acidity	144:161	Control of acidity is critical for cheese quality, as high acidity can be associated with poor flavor and textural attributes.
31864749	9	32	theme	lactic	1594:1599	arg1	acid					1601:1604	lactic acid	1594:1604	lactic acid	1594:1604	Cheeses made with LS exhibited lower lactose and lactic acid contents than WD30 and WD15, leading to significantly higher pH values in the cheese.
31864749	4	33	theme	lactose	693:699	arg1	content					701:707	the lactose content	689:707	the lactose content of milk during the ultrafiltration process	689:750	Direct standardization of the lactose content of milk during the ultrafiltration process could be a simpler and more effective technique to control cheese acidity.
31864749	0	34	theme	lactose	76:82	arg1	standardization					84:98	lactose standardization	76:98	lactose standardization of cheesemilk and whey dilution techniques	76:141	Low- and reduced-fat milled curd, direct-salted Gouda cheese: Comparison of lactose standardization of cheesemilk and whey dilution techniques.
31864749	12	35	theme	Sensory	2009:2015	arg1	analysis					2017:2024	Sensory analysis	2009:2024	Sensory analysis	2009:2024	Sensory analysis also indicated that LS cheeses had lower acidity and softer texture.
31864749	2	36	dep	low-fat	352:358	arg1	curd					393:396	milled curd	386:396	milled curd	386:396	We investigated an alternative method to control cheese acidity, specifically in low-fat (LF) and reduced-fat (RF) milled curd, direct-salted Gouda cheese, which involved altering the initial lactose content of cheesemilk.
31864749	5	37	theme	content	986:992	arg1	adjustment					960:969	adjustment	960:969	adjustment of the lactose content of milk using low-concentration-factor ultrafiltration (LCF-UF)	960:1056	This study compared the effect of traditional WD at 2 different levels, 15 and 30% (WD15 and WD30), with the alternative approach of adjustment of the lactose content of milk using low-concentration-factor ultrafiltration (LCF-UF).
31864749	2	38	theme	direct-salted	399:411	arg1	cheese					419:424	direct-salted Gouda cheese	399:424	direct-salted Gouda cheese	399:424	We investigated an alternative method to control cheese acidity, specifically in low-fat (LF) and reduced-fat (RF) milled curd, direct-salted Gouda cheese, which involved altering the initial lactose content of cheesemilk.
31864749	11	39	theme	CN	1984:1985	arg1	ratio					1987:1991	the lower L:CN ratio	1972:1991	the lower L:CN ratio in these milks	1972:2006	Cheeses made with LS had lower insoluble Ca contents, likely caused by the addition of water required to achieve the lower L:CN ratio in these milks.
31864749	4	40	theme	Direct	663:668	arg1	standardization					670:684	Direct standardization	663:684	Direct standardization of the lactose content of milk during the ultrafiltration process	663:750	Direct standardization of the lactose content of milk during the ultrafiltration process could be a simpler and more effective technique to control cheese acidity.
31864749	4	40	theme	Direct	663:668	arg1	technique					790:798	a simpler and more effective technique	761:798	a simpler and more effective technique to control cheese acidity	761:824	Direct standardization of the lactose content of milk during the ultrafiltration process could be a simpler and more effective technique to control cheese acidity.
31864749	3	41	from	dilution	583:590	arg1	manufacture					522:532	traditional Gouda cheese manufacture	497:532	traditional Gouda cheese manufacture	497:532	In traditional Gouda cheese manufacture, a critical technique to control acidity is whey dilution (WD); that is, partial removal of whey and its replacement with water.
31864749	1	42	theme	textural	250:257	arg1	attributes					259:268	poor flavor and textural attributes	234:268	poor flavor and textural attributes	234:268	Control of acidity is critical for cheese quality, as high acidity can be associated with poor flavor and textural attributes.
31864749	5	43	theme	WD	873:874	arg1	effect					851:856	the effect	847:856	the effect of traditional WD at 2 different levels, 15 and 30% (WD15 and WD30),	847:925	This study compared the effect of traditional WD at 2 different levels, 15 and 30% (WD15 and WD30), with the alternative approach of adjustment of the lactose content of milk using low-concentration-factor ultrafiltration (LCF-UF).
31864749	7	44	theme	cheese	1201:1206	arg1	protocol					1222:1229	A milled curd, direct-salted cheese manufacturing protocol	1172:1229	A milled curd, direct-salted cheese manufacturing protocol	1172:1229	A milled curd, direct-salted cheese manufacturing protocol was used.
31864749	8	45	theme	lactose-to-casein	1275:1291	arg1	ratio					1300:1304	a lactose-to-casein (L:CN) ratio	1273:1304	a lactose-to-casein (L:CN) ratio of approximately 1.8, which is the typical ratio found in milk	1273:1367	Milks used for cheesemaking had a lactose-to-casein (L:CN) ratio of approximately 1.8, which is the typical ratio found in milk, whereas milks prepared with lactose standardization (LS) were made from UF concentrated milks with water added during filtration to achieve a L:CN ratio of approximately 1.1.
31864749	0	46	theme	curd	28:31	arg1	cheese					54:59	curd, direct-salted Gouda cheese	28:59	curd, direct-salted Gouda cheese	28:59	Low- and reduced-fat milled curd, direct-salted Gouda cheese: Comparison of lactose standardization of cheesemilk and whey dilution techniques.
31864749	14	47	used	used	2278:2281	arg2	LS					2266:2267	LS	2266:2267	LS	2266:2267	In addition, LS could be used to help soften texture and increase meltability, if desired in lower-fat cheese types.
31864749	13	48	theme	L	2145:2145	arg1	ratio					2150:2154	the L:CN ratio	2141:2154	the L:CN ratio of milk	2141:2162	These results suggest that standardization of the L:CN ratio of milk could be a useful alternative to WD (or a curd rinse step) to reduce acidity in cheeses.
31864749	7	49	theme	milled	1174:1179	arg1	protocol					1222:1229	A milled curd, direct-salted cheese manufacturing protocol	1172:1229	A milled curd, direct-salted cheese manufacturing protocol	1172:1229	A milled curd, direct-salted cheese manufacturing protocol was used.
31864749	8	50	contain	had	1269:1271	arg2	ratio					1300:1304	a lactose-to-casein (L:CN) ratio	1273:1304	a lactose-to-casein (L:CN) ratio of approximately 1.8, which is the typical ratio found in milk	1273:1367	Milks used for cheesemaking had a lactose-to-casein (L:CN) ratio of approximately 1.8, which is the typical ratio found in milk, whereas milks prepared with lactose standardization (LS) were made from UF concentrated milks with water added during filtration to achieve a L:CN ratio of approximately 1.1.
31864749	8	50	contain	had	1269:1271	arg1	Milks					1241:1245	Milks	1241:1245	Milks used for cheesemaking	1241:1267	Milks used for cheesemaking had a lactose-to-casein (L:CN) ratio of approximately 1.8, which is the typical ratio found in milk, whereas milks prepared with lactose standardization (LS) were made from UF concentrated milks with water added during filtration to achieve a L:CN ratio of approximately 1.1.
31864749	0	51	dep	curd	28:31	arg1	direct-salted					34:46	direct-salted	34:46	direct-salted	34:46	Low- and reduced-fat milled curd, direct-salted Gouda cheese: Comparison of lactose standardization of cheesemilk and whey dilution techniques.
31864749	9	52	theme	pH	1667:1668	arg1	values					1670:1675	significantly higher pH values	1646:1675	significantly higher pH values in the cheese	1646:1689	Cheeses made with LS exhibited lower lactose and lactic acid contents than WD30 and WD15, leading to significantly higher pH values in the cheese.
31864749	3	53	theme	partial	607:613	arg1	removal					615:621	partial removal	607:621	partial removal of whey	607:629	In traditional Gouda cheese manufacture, a critical technique to control acidity is whey dilution (WD); that is, partial removal of whey and its replacement with water.
31864749	3	54	with	replacement	639:649	arg1	water					656:660	water	656:660	water	656:660	In traditional Gouda cheese manufacture, a critical technique to control acidity is whey dilution (WD); that is, partial removal of whey and its replacement with water.
31864749	11	55	from	ratio	1987:1991	arg1	milks					2002:2006	these milks	1996:2006	these milks	1996:2006	Cheeses made with LS had lower insoluble Ca contents, likely caused by the addition of water required to achieve the lower L:CN ratio in these milks.
31864749	4	56	theme	ultrafiltration	728:742	arg1	process					744:750	the ultrafiltration process	724:750	the ultrafiltration process	724:750	Direct standardization of the lactose content of milk during the ultrafiltration process could be a simpler and more effective technique to control cheese acidity.
31864749	14	57	theme	cheese	2356:2361	arg1	types					2363:2367	lower-fat cheese types	2346:2367	lower-fat cheese types	2346:2367	In addition, LS could be used to help soften texture and increase meltability, if desired in lower-fat cheese types.
31864749	8	58	theme	concentrated	1445:1456	arg1	milks					1458:1462	UF concentrated milks	1442:1462	UF concentrated milks with water added during filtration	1442:1497	Milks used for cheesemaking had a lactose-to-casein (L:CN) ratio of approximately 1.8, which is the typical ratio found in milk, whereas milks prepared with lactose standardization (LS) were made from UF concentrated milks with water added during filtration to achieve a L:CN ratio of approximately 1.1.
31864749	11	59	theme	L	1982:1982	arg1	ratio					1987:1991	the lower L:CN ratio	1972:1991	the lower L:CN ratio in these milks	1972:2006	Cheeses made with LS had lower insoluble Ca contents, likely caused by the addition of water required to achieve the lower L:CN ratio in these milks.
31864749	8	60	theme	L	1512:1512	arg1	ratio					1517:1521	a L:CN ratio	1510:1521	a L:CN ratio of approximately 1.1	1510:1542	Milks used for cheesemaking had a lactose-to-casein (L:CN) ratio of approximately 1.8, which is the typical ratio found in milk, whereas milks prepared with lactose standardization (LS) were made from UF concentrated milks with water added during filtration to achieve a L:CN ratio of approximately 1.1.
31864749	10	61	theme	oscillatory	1716:1726	arg1	rheology					1728:1735	Dynamic small-amplitude oscillatory rheology	1692:1735	Dynamic small-amplitude oscillatory rheology	1692:1735	Dynamic small-amplitude oscillatory rheology indicated that use of LS led to cheeses with a lower crossover temperature (melting point) than the cheeses made with WD.
31864749	3	62	theme	traditional	497:507	arg1	manufacture					522:532	traditional Gouda cheese manufacture	497:532	traditional Gouda cheese manufacture	497:532	In traditional Gouda cheese manufacture, a critical technique to control acidity is whey dilution (WD); that is, partial removal of whey and its replacement with water.
31864749	5	63	from	levels	891:896	arg1	effect					851:856	the effect	847:856	the effect of traditional WD at 2 different levels, 15 and 30% (WD15 and WD30),	847:925	This study compared the effect of traditional WD at 2 different levels, 15 and 30% (WD15 and WD30), with the alternative approach of adjustment of the lactose content of milk using low-concentration-factor ultrafiltration (LCF-UF).
31864749	1	64	theme	flavor	239:244	arg1	attributes					259:268	poor flavor and textural attributes	234:268	poor flavor and textural attributes	234:268	Control of acidity is critical for cheese quality, as high acidity can be associated with poor flavor and textural attributes.
31864749	11	65	theme	Ca	1900:1901	arg1	contents					1903:1910	lower insoluble Ca contents	1884:1910	lower insoluble Ca contents	1884:1910	Cheeses made with LS had lower insoluble Ca contents, likely caused by the addition of water required to achieve the lower L:CN ratio in these milks.
31864749	10	66	theme	LS	1759:1760	arg1	use					1752:1754	use	1752:1754	use of LS	1752:1760	Dynamic small-amplitude oscillatory rheology indicated that use of LS led to cheeses with a lower crossover temperature (melting point) than the cheeses made with WD.
31864749	6	67	theme	Gouda	1142:1146	arg1	cheeses					1148:1154	these LF and RF Gouda cheeses	1126:1154	cheeses	1148:1154	The composition, texture, functionality, and sensory properties of these LF and RF Gouda cheeses were evaluated.
31864749	8	68	dep	had	1269:1271	arg1	whereas					1370:1376	whereas	1370:1376	whereas	1370:1376	Milks used for cheesemaking had a lactose-to-casein (L:CN) ratio of approximately 1.8, which is the typical ratio found in milk, whereas milks prepared with lactose standardization (LS) were made from UF concentrated milks with water added during filtration to achieve a L:CN ratio of approximately 1.1.
31864749	9	69	theme	higher	1660:1665	arg1	values					1670:1675	significantly higher pH values	1646:1675	significantly higher pH values in the cheese	1646:1689	Cheeses made with LS exhibited lower lactose and lactic acid contents than WD30 and WD15, leading to significantly higher pH values in the cheese.
31864749	4	70	theme	simpler	763:769	arg1	standardization					670:684	Direct standardization	663:684	Direct standardization of the lactose content of milk during the ultrafiltration process	663:750	Direct standardization of the lactose content of milk during the ultrafiltration process could be a simpler and more effective technique to control cheese acidity.
31864749	4	70	theme	simpler	763:769	arg1	technique					790:798	a simpler and more effective technique	761:798	a simpler and more effective technique to control cheese acidity	761:824	Direct standardization of the lactose content of milk during the ultrafiltration process could be a simpler and more effective technique to control cheese acidity.
31864749	10	71	theme	Dynamic	1692:1698	arg1	rheology					1728:1735	Dynamic small-amplitude oscillatory rheology	1692:1735	Dynamic small-amplitude oscillatory rheology	1692:1735	Dynamic small-amplitude oscillatory rheology indicated that use of LS led to cheeses with a lower crossover temperature (melting point) than the cheeses made with WD.
31864749	8	72	theme	1.1	1540:1542	arg1	ratio					1517:1521	a L:CN ratio	1510:1521	a L:CN ratio of approximately 1.1	1510:1542	Milks used for cheesemaking had a lactose-to-casein (L:CN) ratio of approximately 1.8, which is the typical ratio found in milk, whereas milks prepared with lactose standardization (LS) were made from UF concentrated milks with water added during filtration to achieve a L:CN ratio of approximately 1.1.
31864749	0	73	theme	whey	118:121	arg1	techniques					132:141	whey dilution techniques	118:141	whey dilution techniques	118:141	Low- and reduced-fat milled curd, direct-salted Gouda cheese: Comparison of lactose standardization of cheesemilk and whey dilution techniques.
31864749	13	74	theme	CN	2147:2148	arg1	ratio					2150:2154	the L:CN ratio	2141:2154	the L:CN ratio of milk	2141:2162	These results suggest that standardization of the L:CN ratio of milk could be a useful alternative to WD (or a curd rinse step) to reduce acidity in cheeses.
31864749	3	75	theme	cheese	515:520	arg1	manufacture					522:532	traditional Gouda cheese manufacture	497:532	traditional Gouda cheese manufacture	497:532	In traditional Gouda cheese manufacture, a critical technique to control acidity is whey dilution (WD); that is, partial removal of whey and its replacement with water.
31864749	9	76	from	values	1670:1675	arg1	cheese					1684:1689	the cheese	1680:1689	the cheese	1680:1689	Cheeses made with LS exhibited lower lactose and lactic acid contents than WD30 and WD15, leading to significantly higher pH values in the cheese.
31864749	0	77	theme	techniques	132:141	arg1	standardization					84:98	lactose standardization	76:98	lactose standardization of cheesemilk and whey dilution techniques	76:141	Low- and reduced-fat milled curd, direct-salted Gouda cheese: Comparison of lactose standardization of cheesemilk and whey dilution techniques.
31864749	2	78	theme	cheesemilk	482:491	arg1	content					471:477	the initial lactose content	451:477	the initial lactose content of cheesemilk	451:491	We investigated an alternative method to control cheese acidity, specifically in low-fat (LF) and reduced-fat (RF) milled curd, direct-salted Gouda cheese, which involved altering the initial lactose content of cheesemilk.
31864749	3	79	theme	critical	537:544	arg1	technique					546:554	a critical technique	535:554	a critical technique to control acidity	535:573	In traditional Gouda cheese manufacture, a critical technique to control acidity is whey dilution (WD); that is, partial removal of whey and its replacement with water.
31864749	3	79	theme	critical	537:544	arg1	dilution					583:590	whey dilution	578:590	whey dilution (WD)	578:595	In traditional Gouda cheese manufacture, a critical technique to control acidity is whey dilution (WD); that is, partial removal of whey and its replacement with water.
31864749	11	80	theme	lower	1884:1888	arg1	contents					1903:1910	lower insoluble Ca contents	1884:1910	lower insoluble Ca contents	1884:1910	Cheeses made with LS had lower insoluble Ca contents, likely caused by the addition of water required to achieve the lower L:CN ratio in these milks.
31864749	6	81	theme	sensory	1104:1110	arg1	properties					1112:1121	sensory properties	1104:1121	sensory properties	1104:1121	The composition, texture, functionality, and sensory properties of these LF and RF Gouda cheeses were evaluated.
31864749	10	82	theme	lower	1784:1788	arg1	temperature					1800:1810	a lower crossover temperature	1782:1810	a lower crossover temperature (melting point) than the cheeses made with WD	1782:1856	Dynamic small-amplitude oscillatory rheology indicated that use of LS led to cheeses with a lower crossover temperature (melting point) than the cheeses made with WD.
31864749	10	82	theme	lower	1784:1788	arg1	point					1821:1825	melting point	1813:1825	melting point	1813:1825	Dynamic small-amplitude oscillatory rheology indicated that use of LS led to cheeses with a lower crossover temperature (melting point) than the cheeses made with WD.
31864749	3	83	theme	whey	578:581	arg1	WD					593:594	WD	593:594	WD	593:594	In traditional Gouda cheese manufacture, a critical technique to control acidity is whey dilution (WD); that is, partial removal of whey and its replacement with water.
31864749	3	83	theme	whey	578:581	arg1	technique					546:554	a critical technique	535:554	a critical technique to control acidity	535:573	In traditional Gouda cheese manufacture, a critical technique to control acidity is whey dilution (WD); that is, partial removal of whey and its replacement with water.
31864749	3	83	theme	whey	578:581	arg1	dilution					583:590	whey dilution	578:590	whey dilution (WD)	578:595	In traditional Gouda cheese manufacture, a critical technique to control acidity is whey dilution (WD); that is, partial removal of whey and its replacement with water.
31864749	8	84	theme	typical	1341:1347	arg1	1.8					1323:1325	1.8	1323:1325	1.8	1323:1325	Milks used for cheesemaking had a lactose-to-casein (L:CN) ratio of approximately 1.8, which is the typical ratio found in milk, whereas milks prepared with lactose standardization (LS) were made from UF concentrated milks with water added during filtration to achieve a L:CN ratio of approximately 1.1.
31864749	8	84	theme	typical	1341:1347	arg1	ratio					1349:1353	the typical ratio	1337:1353	the typical ratio found in milk	1337:1367	Milks used for cheesemaking had a lactose-to-casein (L:CN) ratio of approximately 1.8, which is the typical ratio found in milk, whereas milks prepared with lactose standardization (LS) were made from UF concentrated milks with water added during filtration to achieve a L:CN ratio of approximately 1.1.
31864749	11	85	contain	had	1880:1882	arg1	Cheeses					1859:1865	Cheeses	1859:1865	Cheeses made with LS	1859:1878	Cheeses made with LS had lower insoluble Ca contents, likely caused by the addition of water required to achieve the lower L:CN ratio in these milks.
31864749	11	85	contain	had	1880:1882	arg2	contents					1903:1910	lower insoluble Ca contents	1884:1910	lower insoluble Ca contents	1884:1910	Cheeses made with LS had lower insoluble Ca contents, likely caused by the addition of water required to achieve the lower L:CN ratio in these milks.
31864749	5	86	theme	alternative	936:946	arg1	approach					948:955	the alternative approach	932:955	the alternative approach of adjustment of the lactose content of milk using low-concentration-factor ultrafiltration (LCF-UF)	932:1056	This study compared the effect of traditional WD at 2 different levels, 15 and 30% (WD15 and WD30), with the alternative approach of adjustment of the lactose content of milk using low-concentration-factor ultrafiltration (LCF-UF).
31864749	8	87	dep	L	1294:1294	arg1	CN					1296:1297	CN	1296:1297	L:CN	1294:1297	Milks used for cheesemaking had a lactose-to-casein (L:CN) ratio of approximately 1.8, which is the typical ratio found in milk, whereas milks prepared with lactose standardization (LS) were made from UF concentrated milks with water added during filtration to achieve a L:CN ratio of approximately 1.1.
31864749	9	88	theme	lower	1576:1580	arg1	contents					1606:1613	lower lactose and lactic acid contents	1576:1613	lower lactose and lactic acid contents	1576:1613	Cheeses made with LS exhibited lower lactose and lactic acid contents than WD30 and WD15, leading to significantly higher pH values in the cheese.
31864749	12	89	theme	LS	2046:2047	arg1	cheeses					2049:2055	LS cheeses	2046:2055	LS cheeses	2046:2055	Sensory analysis also indicated that LS cheeses had lower acidity and softer texture.
31864749	10	90	theme	melting	1813:1819	arg1	temperature					1800:1810	a lower crossover temperature	1782:1810	a lower crossover temperature (melting point) than the cheeses made with WD	1782:1856	Dynamic small-amplitude oscillatory rheology indicated that use of LS led to cheeses with a lower crossover temperature (melting point) than the cheeses made with WD.
31864749	10	90	theme	melting	1813:1819	arg1	point					1821:1825	melting point	1813:1825	melting point	1813:1825	Dynamic small-amplitude oscillatory rheology indicated that use of LS led to cheeses with a lower crossover temperature (melting point) than the cheeses made with WD.
31864749	8	91	dep	lactose-to-casein	1275:1291	arg1	L					1294:1294	L	1294:1294	L:CN	1294:1297	Milks used for cheesemaking had a lactose-to-casein (L:CN) ratio of approximately 1.8, which is the typical ratio found in milk, whereas milks prepared with lactose standardization (LS) were made from UF concentrated milks with water added during filtration to achieve a L:CN ratio of approximately 1.1.
31864749	0	92	theme	standardization	84:98	arg1	Comparison					62:71	Comparison	62:71	Comparison of lactose standardization of cheesemilk and whey dilution techniques	62:141	Low- and reduced-fat milled curd, direct-salted Gouda cheese: Comparison of lactose standardization of cheesemilk and whey dilution techniques.
31864749	5	93	theme	different	881:889	arg1	%					908:908	15 and 30%	899:908	15 and 30% (WD15 and WD30)	899:924	This study compared the effect of traditional WD at 2 different levels, 15 and 30% (WD15 and WD30), with the alternative approach of adjustment of the lactose content of milk using low-concentration-factor ultrafiltration (LCF-UF).
31864749	5	93	theme	different	881:889	arg1	levels					891:896	2 different levels	879:896	2 different levels	879:896	This study compared the effect of traditional WD at 2 different levels, 15 and 30% (WD15 and WD30), with the alternative approach of adjustment of the lactose content of milk using low-concentration-factor ultrafiltration (LCF-UF).
31864749	9	94	theme	acid	1601:1604	arg1	contents					1606:1613	lower lactose and lactic acid contents	1576:1613	lower lactose and lactic acid contents	1576:1613	Cheeses made with LS exhibited lower lactose and lactic acid contents than WD30 and WD15, leading to significantly higher pH values in the cheese.
31864749	0	95	theme	cheesemilk	103:112	arg1	standardization					84:98	lactose standardization	76:98	lactose standardization of cheesemilk and whey dilution techniques	76:141	Low- and reduced-fat milled curd, direct-salted Gouda cheese: Comparison of lactose standardization of cheesemilk and whey dilution techniques.
31864749	5	96	theme	lactose	978:984	arg1	content					986:992	the lactose content	974:992	the lactose content of milk using low-concentration-factor ultrafiltration (LCF-UF)	974:1056	This study compared the effect of traditional WD at 2 different levels, 15 and 30% (WD15 and WD30), with the alternative approach of adjustment of the lactose content of milk using low-concentration-factor ultrafiltration (LCF-UF).
31864749	13	97	theme	useful	2175:2180	arg1	standardization					2122:2136	standardization	2122:2136	standardization of the L:CN ratio of milk	2122:2162	These results suggest that standardization of the L:CN ratio of milk could be a useful alternative to WD (or a curd rinse step) to reduce acidity in cheeses.
31864749	13	97	theme	useful	2175:2180	arg1	alternative					2182:2192	a useful alternative	2173:2192	a useful alternative to WD (or a curd rinse step) to reduce acidity in cheeses	2173:2250	These results suggest that standardization of the L:CN ratio of milk could be a useful alternative to WD (or a curd rinse step) to reduce acidity in cheeses.
31864749	8	98	located	found	1355:1359	arg2	ratio					1349:1353	the typical ratio	1337:1353	the typical ratio found in milk	1337:1367	Milks used for cheesemaking had a lactose-to-casein (L:CN) ratio of approximately 1.8, which is the typical ratio found in milk, whereas milks prepared with lactose standardization (LS) were made from UF concentrated milks with water added during filtration to achieve a L:CN ratio of approximately 1.1.
31864749	8	98	located	found	1355:1359	arg1	milk					1364:1367	milk	1364:1367	milk	1364:1367	Milks used for cheesemaking had a lactose-to-casein (L:CN) ratio of approximately 1.8, which is the typical ratio found in milk, whereas milks prepared with lactose standardization (LS) were made from UF concentrated milks with water added during filtration to achieve a L:CN ratio of approximately 1.1.
31864749	8	98	located	found	1355:1359	arg2	1.8					1323:1325	1.8	1323:1325	1.8	1323:1325	Milks used for cheesemaking had a lactose-to-casein (L:CN) ratio of approximately 1.8, which is the typical ratio found in milk, whereas milks prepared with lactose standardization (LS) were made from UF concentrated milks with water added during filtration to achieve a L:CN ratio of approximately 1.1.
31864749	7	99	theme	manufacturing	1208:1220	arg1	protocol					1222:1229	A milled curd, direct-salted cheese manufacturing protocol	1172:1229	A milled curd, direct-salted cheese manufacturing protocol	1172:1229	A milled curd, direct-salted cheese manufacturing protocol was used.
31864749	2	100	theme	lactose	463:469	arg1	content					471:477	the initial lactose content	451:477	the initial lactose content of cheesemilk	451:491	We investigated an alternative method to control cheese acidity, specifically in low-fat (LF) and reduced-fat (RF) milled curd, direct-salted Gouda cheese, which involved altering the initial lactose content of cheesemilk.
31864749	4	101	theme	content	701:707	arg1	standardization					670:684	Direct standardization	663:684	Direct standardization of the lactose content of milk during the ultrafiltration process	663:750	Direct standardization of the lactose content of milk during the ultrafiltration process could be a simpler and more effective technique to control cheese acidity.
31864749	4	101	theme	content	701:707	arg1	technique					790:798	a simpler and more effective technique	761:798	a simpler and more effective technique to control cheese acidity	761:824	Direct standardization of the lactose content of milk during the ultrafiltration process could be a simpler and more effective technique to control cheese acidity.
31864749	5	102	theme	traditional	861:871	arg1	WD					873:874	traditional WD	861:874	traditional WD	861:874	This study compared the effect of traditional WD at 2 different levels, 15 and 30% (WD15 and WD30), with the alternative approach of adjustment of the lactose content of milk using low-concentration-factor ultrafiltration (LCF-UF).
31864749	13	103	theme	rinse	2211:2215	arg1	WD					2197:2198	WD	2197:2198	WD (or a curd rinse step)	2197:2221	These results suggest that standardization of the L:CN ratio of milk could be a useful alternative to WD (or a curd rinse step) to reduce acidity in cheeses.
31864749	13	103	theme	rinse	2211:2215	arg1	step					2217:2220	a curd rinse step	2204:2220	a curd rinse step	2204:2220	These results suggest that standardization of the L:CN ratio of milk could be a useful alternative to WD (or a curd rinse step) to reduce acidity in cheeses.
31864749	8	104	theme	lactose	1398:1404	arg1	standardization					1406:1420	lactose standardization	1398:1420	lactose standardization (LS)	1398:1425	Milks used for cheesemaking had a lactose-to-casein (L:CN) ratio of approximately 1.8, which is the typical ratio found in milk, whereas milks prepared with lactose standardization (LS) were made from UF concentrated milks with water added during filtration to achieve a L:CN ratio of approximately 1.1.
31864749	8	104	theme	lactose	1398:1404	arg1	LS					1423:1424	LS	1423:1424	LS	1423:1424	Milks used for cheesemaking had a lactose-to-casein (L:CN) ratio of approximately 1.8, which is the typical ratio found in milk, whereas milks prepared with lactose standardization (LS) were made from UF concentrated milks with water added during filtration to achieve a L:CN ratio of approximately 1.1.
31864749	13	105	theme	milk	2159:2162	arg1	ratio					2150:2154	the L:CN ratio	2141:2154	the L:CN ratio of milk	2141:2162	These results suggest that standardization of the L:CN ratio of milk could be a useful alternative to WD (or a curd rinse step) to reduce acidity in cheeses.
31864749	7	106	theme	curd	1181:1184	arg1	protocol					1222:1229	A milled curd, direct-salted cheese manufacturing protocol	1172:1229	A milled curd, direct-salted cheese manufacturing protocol	1172:1229	A milled curd, direct-salted cheese manufacturing protocol was used.
31864749	2	107	theme	milled	386:391	arg1	curd					393:396	milled curd	386:396	milled curd	386:396	We investigated an alternative method to control cheese acidity, specifically in low-fat (LF) and reduced-fat (RF) milled curd, direct-salted Gouda cheese, which involved altering the initial lactose content of cheesemilk.
31864749	11	108	theme	lower	1976:1980	arg1	ratio					1987:1991	the lower L:CN ratio	1972:1991	the lower L:CN ratio in these milks	1972:2006	Cheeses made with LS had lower insoluble Ca contents, likely caused by the addition of water required to achieve the lower L:CN ratio in these milks.
31864749	0	109	theme	Gouda	48:52	arg1	cheese					54:59	curd, direct-salted Gouda cheese	28:59	curd, direct-salted Gouda cheese	28:59	Low- and reduced-fat milled curd, direct-salted Gouda cheese: Comparison of lactose standardization of cheesemilk and whey dilution techniques.
31864749	2	110	theme	Gouda	413:417	arg1	cheese					419:424	direct-salted Gouda cheese	399:424	direct-salted Gouda cheese	399:424	We investigated an alternative method to control cheese acidity, specifically in low-fat (LF) and reduced-fat (RF) milled curd, direct-salted Gouda cheese, which involved altering the initial lactose content of cheesemilk.
31864749	10	111	theme	small-amplitude	1700:1714	arg1	rheology					1728:1735	Dynamic small-amplitude oscillatory rheology	1692:1735	Dynamic small-amplitude oscillatory rheology	1692:1735	Dynamic small-amplitude oscillatory rheology indicated that use of LS led to cheeses with a lower crossover temperature (melting point) than the cheeses made with WD.
31864749	2	112	theme	cheese	320:325	arg1	acidity					327:333	cheese acidity	320:333	cheese acidity	320:333	We investigated an alternative method to control cheese acidity, specifically in low-fat (LF) and reduced-fat (RF) milled curd, direct-salted Gouda cheese, which involved altering the initial lactose content of cheesemilk.
31864749	0	113	dep	milled	21:26	arg1	Comparison					62:71	Comparison	62:71	Comparison of lactose standardization of cheesemilk and whey dilution techniques	62:141	Low- and reduced-fat milled curd, direct-salted Gouda cheese: Comparison of lactose standardization of cheesemilk and whey dilution techniques.
31864749	8	114	theme	UF	1442:1443	arg1	milks					1458:1462	UF concentrated milks	1442:1462	UF concentrated milks with water added during filtration	1442:1497	Milks used for cheesemaking had a lactose-to-casein (L:CN) ratio of approximately 1.8, which is the typical ratio found in milk, whereas milks prepared with lactose standardization (LS) were made from UF concentrated milks with water added during filtration to achieve a L:CN ratio of approximately 1.1.
31864749	4	115	theme	milk	712:715	arg1	content					701:707	the lactose content	689:707	the lactose content of milk during the ultrafiltration process	689:750	Direct standardization of the lactose content of milk during the ultrafiltration process could be a simpler and more effective technique to control cheese acidity.
31864749	7	116	dep	curd	1181:1184	arg1	direct-salted					1187:1199	direct-salted	1187:1199	direct-salted	1187:1199	A milled curd, direct-salted cheese manufacturing protocol was used.
31864749	3	117	theme	whey	626:629	arg1	replacement					639:649	its replacement	635:649	its replacement with water	635:660	In traditional Gouda cheese manufacture, a critical technique to control acidity is whey dilution (WD); that is, partial removal of whey and its replacement with water.
31864749	3	117	theme	whey	626:629	arg1	removal					615:621	partial removal	607:621	partial removal of whey	607:629	In traditional Gouda cheese manufacture, a critical technique to control acidity is whey dilution (WD); that is, partial removal of whey and its replacement with water.
31864749	11	118	theme	water	1946:1950	arg1	addition					1934:1941	the addition	1930:1941	the addition of water required to achieve the lower L:CN ratio in these milks	1930:2006	Cheeses made with LS had lower insoluble Ca contents, likely caused by the addition of water required to achieve the lower L:CN ratio in these milks.
31864749	3	119	with	removal	615:621	arg1	water					656:660	water	656:660	water	656:660	In traditional Gouda cheese manufacture, a critical technique to control acidity is whey dilution (WD); that is, partial removal of whey and its replacement with water.
31864749	12	120	dep	lower	2061:2065	arg1	acidity					2067:2073	acidity	2067:2073	acidity	2067:2073	Sensory analysis also indicated that LS cheeses had lower acidity and softer texture.
31864749	12	120	dep	lower	2061:2065	arg1	texture					2086:2092	softer texture	2079:2092	softer texture	2079:2092	Sensory analysis also indicated that LS cheeses had lower acidity and softer texture.
31525200	8	0	theme	high	1228:1231	arg1	percentage					1233:1242	The high percentage	1224:1242	The high percentage of oxygenated monoterpenes in both essential oils	1224:1292	The high percentage of oxygenated monoterpenes in both essential oils play an important role in the antioxidant and wound healing potential observed herein.
31525200	5	1	theme	oxygenated	676:685	arg1	monoterpenes					687:698	oxygenated monoterpenes	676:698	oxygenated monoterpenes	676:698	GC/MS analysis of M. alternifolia and R. officinalis essential oils revealed richness in oxygenated monoterpenes, representing 51.06% and 69.61% of the total oil composition, respectively.
31525200	2	2	theme	Essential	354:362	arg1	oils					364:367	Essential oils	354:367	Essential oils of M. alternifolia and R. officinalis	354:405	Essential oils of M. alternifolia and R. officinalis were analyzed using GC/MS.
31525200	1	3	theme	essential	281:289	arg1	oil					291:293	tea tree essential oil	272:293	tea tree essential oil	272:293	The present study investigates the wound healing potential of three chitosan-based topical preparations loaded with either tea tree essential oil, rosemary essential oil or a mixture of both oils in vivo.
31525200	6	4	theme	contraction	922:932	arg1	percentage					934:943	wound contraction percentage	916:943	wound contraction percentage	916:943	Topical application of chitosan-based formulation loaded with a mixture of tea tree and rosemary oils resulted in a significant increase in wound contraction percentage compared to either group treated with individual essential oils and the untreated group.
31525200	9	5	theme	healing	1545:1551	arg1	stages					1529:1534	different stages	1519:1534	different stages of wound healing	1519:1551	Incorporation of tea tree and rosemary essential oils in chitosan-based preparations in appropriate combination could efficiently promote different stages of wound healing.
31525200	8	6	theme	monoterpenes	1258:1269	arg1	percentage					1233:1242	The high percentage	1224:1242	The high percentage of oxygenated monoterpenes in both essential oils	1224:1292	The high percentage of oxygenated monoterpenes in both essential oils play an important role in the antioxidant and wound healing potential observed herein.
31525200	1	7	theme	healing	190:196	arg1	potential					198:206	the wound healing potential	180:206	the wound healing potential of three chitosan-based topical preparations loaded with either tea tree essential oil, rosemary essential oil or a mixture of both oils in vivo	180:351	The present study investigates the wound healing potential of three chitosan-based topical preparations loaded with either tea tree essential oil, rosemary essential oil or a mixture of both oils in vivo.
31525200	6	8	theme	significant	892:902	arg1	increase					904:911	a significant increase	890:911	a significant increase in wound contraction percentage	890:943	Topical application of chitosan-based formulation loaded with a mixture of tea tree and rosemary oils resulted in a significant increase in wound contraction percentage compared to either group treated with individual essential oils and the untreated group.
31525200	9	9	theme	essential	1420:1428	arg1	oils					1430:1433	rosemary essential oils	1411:1433	rosemary essential oils	1411:1433	Incorporation of tea tree and rosemary essential oils in chitosan-based preparations in appropriate combination could efficiently promote different stages of wound healing.
31525200	6	10	theme	chitosan-based	799:812	arg1	formulation					814:824	chitosan-based formulation	799:824	chitosan-based formulation loaded with a mixture of tea tree and rosemary oils	799:876	Topical application of chitosan-based formulation loaded with a mixture of tea tree and rosemary oils resulted in a significant increase in wound contraction percentage compared to either group treated with individual essential oils and the untreated group.
31525200	7	11	theme	hair	1208:1211	arg1	follicles					1213:1221	activated hair follicles	1198:1221	activated hair follicles	1198:1221	Histopathological examination revealed that topical application of tea tree and rosemary oil combination demonstrated complete re-epithelialization associated with activated hair follicles.
31525200	8	12	dep	antioxidant	1324:1334	arg1	potential					1354:1362	potential	1354:1362	potential	1354:1362	The high percentage of oxygenated monoterpenes in both essential oils play an important role in the antioxidant and wound healing potential observed herein.
31525200	0	13	theme	topical	127:133	arg1	preparations					135:146	essential oil-loaded chitosan topical preparations	97:146	essential oil-loaded chitosan topical preparations	97:146	Appraisal on the wound healing potential of Melaleuca alternifolia and Rosmarinus officinalis L. essential oil-loaded chitosan topical preparations.
31525200	1	14	theme	chitosan-based	217:230	arg1	preparations					240:251	three chitosan-based topical preparations	211:251	three chitosan-based topical preparations loaded with either tea tree essential oil, rosemary essential oil or a mixture of both oils in vivo	211:351	The present study investigates the wound healing potential of three chitosan-based topical preparations loaded with either tea tree essential oil, rosemary essential oil or a mixture of both oils in vivo.
31525200	9	15	from	Incorporation	1381:1393	arg1	preparations					1453:1464	chitosan-based preparations	1438:1464	chitosan-based preparations in appropriate combination	1438:1491	Incorporation of tea tree and rosemary essential oils in chitosan-based preparations in appropriate combination could efficiently promote different stages of wound healing.
31525200	6	16	theme	individual	983:992	arg1	oils					1004:1007	individual essential oils	983:1007	individual essential oils	983:1007	Topical application of chitosan-based formulation loaded with a mixture of tea tree and rosemary oils resulted in a significant increase in wound contraction percentage compared to either group treated with individual essential oils and the untreated group.
31525200	3	17	theme	Essential	434:442	arg1	preparations					472:483	Essential oil-loaded chitosan topical preparations	434:483	Essential oil-loaded chitosan topical preparations	434:483	Essential oil-loaded chitosan topical preparations were formulated.
31525200	1	18	theme	preparations	240:251	arg1	potential					198:206	the wound healing potential	180:206	the wound healing potential of three chitosan-based topical preparations loaded with either tea tree essential oil, rosemary essential oil or a mixture of both oils in vivo	180:351	The present study investigates the wound healing potential of three chitosan-based topical preparations loaded with either tea tree essential oil, rosemary essential oil or a mixture of both oils in vivo.
31525200	1	19	theme	essential	305:313	arg1	oil					315:317	rosemary essential oil	296:317	rosemary essential oil	296:317	The present study investigates the wound healing potential of three chitosan-based topical preparations loaded with either tea tree essential oil, rosemary essential oil or a mixture of both oils in vivo.
31525200	7	20	theme	tea	1101:1103	arg1	tree					1105:1108	tea tree	1101:1108	tea tree	1101:1108	Histopathological examination revealed that topical application of tea tree and rosemary oil combination demonstrated complete re-epithelialization associated with activated hair follicles.
31525200	7	21	theme	Histopathological	1034:1050	arg1	examination					1052:1062	Histopathological examination	1034:1062	Histopathological examination	1034:1062	Histopathological examination revealed that topical application of tea tree and rosemary oil combination demonstrated complete re-epithelialization associated with activated hair follicles.
31525200	0	22	theme	alternifolia	54:65	arg1	potential					31:39	the wound healing potential	13:39	the wound healing potential of Melaleuca alternifolia and Rosmarinus officinalis L. essential oil-loaded chitosan topical preparations	13:146	Appraisal on the wound healing potential of Melaleuca alternifolia and Rosmarinus officinalis L. essential oil-loaded chitosan topical preparations.
31525200	1	23	theme	oils	340:343	arg1	oil					315:317	rosemary essential oil	296:317	rosemary essential oil	296:317	The present study investigates the wound healing potential of three chitosan-based topical preparations loaded with either tea tree essential oil, rosemary essential oil or a mixture of both oils in vivo.
31525200	1	23	theme	oils	340:343	arg1	mixture					324:330	a mixture	322:330	a mixture of both oils in vivo	322:351	The present study investigates the wound healing potential of three chitosan-based topical preparations loaded with either tea tree essential oil, rosemary essential oil or a mixture of both oils in vivo.
31525200	1	23	theme	oils	340:343	arg1	oil					291:293	tea tree essential oil	272:293	tea tree essential oil	272:293	The present study investigates the wound healing potential of three chitosan-based topical preparations loaded with either tea tree essential oil, rosemary essential oil or a mixture of both oils in vivo.
31525200	5	24	theme	alternifolia	608:619	arg1	analysis					593:600	GC/MS analysis	587:600	GC/MS analysis of M. alternifolia and R. officinalis essential oils	587:653	GC/MS analysis of M. alternifolia and R. officinalis essential oils revealed richness in oxygenated monoterpenes, representing 51.06% and 69.61% of the total oil composition, respectively.
31525200	0	25	from	Appraisal	0:8	arg1	potential					31:39	the wound healing potential	13:39	the wound healing potential of Melaleuca alternifolia and Rosmarinus officinalis L. essential oil-loaded chitosan topical preparations	13:146	Appraisal on the wound healing potential of Melaleuca alternifolia and Rosmarinus officinalis L. essential oil-loaded chitosan topical preparations.
31525200	0	26	theme	L.	94:95	arg1	potential					31:39	the wound healing potential	13:39	the wound healing potential of Melaleuca alternifolia and Rosmarinus officinalis L. essential oil-loaded chitosan topical preparations	13:146	Appraisal on the wound healing potential of Melaleuca alternifolia and Rosmarinus officinalis L. essential oil-loaded chitosan topical preparations.
31525200	9	27	theme	tea	1398:1400	arg1	tree					1402:1405	tea tree	1398:1405	tea tree	1398:1405	Incorporation of tea tree and rosemary essential oils in chitosan-based preparations in appropriate combination could efficiently promote different stages of wound healing.
31525200	5	28	theme	R.	625:626	arg1	analysis					593:600	GC/MS analysis	587:600	GC/MS analysis of M. alternifolia and R. officinalis essential oils	587:653	GC/MS analysis of M. alternifolia and R. officinalis essential oils revealed richness in oxygenated monoterpenes, representing 51.06% and 69.61% of the total oil composition, respectively.
31525200	2	29	theme	R.	392:393	arg1	officinalis					395:405	M. alternifolia and R. officinalis	372:405	officinalis	395:405	Essential oils of M. alternifolia and R. officinalis were analyzed using GC/MS.
31525200	2	30	theme	officinalis	395:405	arg1	oils					364:367	Essential oils	354:367	Essential oils of M. alternifolia and R. officinalis	354:405	Essential oils of M. alternifolia and R. officinalis were analyzed using GC/MS.
31525200	0	31	theme	oil-loaded	107:116	arg1	preparations					135:146	essential oil-loaded chitosan topical preparations	97:146	essential oil-loaded chitosan topical preparations	97:146	Appraisal on the wound healing potential of Melaleuca alternifolia and Rosmarinus officinalis L. essential oil-loaded chitosan topical preparations.
31525200	5	32	theme	GC/MS	587:591	arg1	analysis					593:600	GC/MS analysis	587:600	GC/MS analysis of M. alternifolia and R. officinalis essential oils	587:653	GC/MS analysis of M. alternifolia and R. officinalis essential oils revealed richness in oxygenated monoterpenes, representing 51.06% and 69.61% of the total oil composition, respectively.
31525200	4	33	theme	healing	508:514	arg1	potential					516:524	Wound healing potential	502:524	Wound healing potential	502:524	Wound healing potential was evaluated in vivo using an excision wound model in rats.
31525200	1	34	theme	rosemary	296:303	arg1	oil					315:317	rosemary essential oil	296:317	rosemary essential oil	296:317	The present study investigates the wound healing potential of three chitosan-based topical preparations loaded with either tea tree essential oil, rosemary essential oil or a mixture of both oils in vivo.
31525200	7	35	theme	complete	1152:1159	arg1	re-epithelialization					1161:1180	complete re-epithelialization	1152:1180	complete re-epithelialization associated with activated hair follicles	1152:1221	Histopathological examination revealed that topical application of tea tree and rosemary oil combination demonstrated complete re-epithelialization associated with activated hair follicles.
31525200	4	36	from	model	572:576	arg1	rats					581:584	rats	581:584	rats	581:584	Wound healing potential was evaluated in vivo using an excision wound model in rats.
31525200	0	37	dep	alternifolia	54:65	arg1	preparations					135:146	essential oil-loaded chitosan topical preparations	97:146	essential oil-loaded chitosan topical preparations	97:146	Appraisal on the wound healing potential of Melaleuca alternifolia and Rosmarinus officinalis L. essential oil-loaded chitosan topical preparations.
31525200	3	38	theme	topical	464:470	arg1	preparations					472:483	Essential oil-loaded chitosan topical preparations	434:483	Essential oil-loaded chitosan topical preparations	434:483	Essential oil-loaded chitosan topical preparations were formulated.
31525200	0	39	theme	healing	23:29	arg1	potential					31:39	the wound healing potential	13:39	the wound healing potential of Melaleuca alternifolia and Rosmarinus officinalis L. essential oil-loaded chitosan topical preparations	13:146	Appraisal on the wound healing potential of Melaleuca alternifolia and Rosmarinus officinalis L. essential oil-loaded chitosan topical preparations.
31525200	6	40	theme	Topical	776:782	arg1	application					784:794	Topical application	776:794	Topical application of chitosan-based formulation loaded with a mixture of tea tree and rosemary oils	776:876	Topical application of chitosan-based formulation loaded with a mixture of tea tree and rosemary oils resulted in a significant increase in wound contraction percentage compared to either group treated with individual essential oils and the untreated group.
31525200	9	41	theme	appropriate	1469:1479	arg1	combination					1481:1491	appropriate combination	1469:1491	appropriate combination	1469:1491	Incorporation of tea tree and rosemary essential oils in chitosan-based preparations in appropriate combination could efficiently promote different stages of wound healing.
31525200	6	42	theme	rosemary	864:871	arg1	oils					873:876	rosemary oils	864:876	rosemary oils	864:876	Topical application of chitosan-based formulation loaded with a mixture of tea tree and rosemary oils resulted in a significant increase in wound contraction percentage compared to either group treated with individual essential oils and the untreated group.
31525200	4	43	theme	excision	557:564	arg1	model					572:576	an excision wound model	554:576	an excision wound model in rats	554:584	Wound healing potential was evaluated in vivo using an excision wound model in rats.
31525200	6	44	theme	tree	855:858	arg1	mixture					840:846	a mixture	838:846	a mixture of tea tree and rosemary oils	838:876	Topical application of chitosan-based formulation loaded with a mixture of tea tree and rosemary oils resulted in a significant increase in wound contraction percentage compared to either group treated with individual essential oils and the untreated group.
31525200	5	45	dep	alternifolia	608:619	arg1	oils					650:653	officinalis essential oils	628:653	officinalis essential oils	628:653	GC/MS analysis of M. alternifolia and R. officinalis essential oils revealed richness in oxygenated monoterpenes, representing 51.06% and 69.61% of the total oil composition, respectively.
31525200	7	46	theme	activated	1198:1206	arg1	follicles					1213:1221	activated hair follicles	1198:1221	activated hair follicles	1198:1221	Histopathological examination revealed that topical application of tea tree and rosemary oil combination demonstrated complete re-epithelialization associated with activated hair follicles.
31525200	1	47	theme	tree	276:279	arg1	oil					291:293	tea tree essential oil	272:293	tea tree essential oil	272:293	The present study investigates the wound healing potential of three chitosan-based topical preparations loaded with either tea tree essential oil, rosemary essential oil or a mixture of both oils in vivo.
31525200	6	48	from	increase	904:911	arg1	percentage					934:943	wound contraction percentage	916:943	wound contraction percentage	916:943	Topical application of chitosan-based formulation loaded with a mixture of tea tree and rosemary oils resulted in a significant increase in wound contraction percentage compared to either group treated with individual essential oils and the untreated group.
31525200	9	49	theme	wound	1539:1543	arg1	healing					1545:1551	wound healing	1539:1551	wound healing	1539:1551	Incorporation of tea tree and rosemary essential oils in chitosan-based preparations in appropriate combination could efficiently promote different stages of wound healing.
31525200	6	50	theme	wound	916:920	arg1	percentage					934:943	wound contraction percentage	916:943	wound contraction percentage	916:943	Topical application of chitosan-based formulation loaded with a mixture of tea tree and rosemary oils resulted in a significant increase in wound contraction percentage compared to either group treated with individual essential oils and the untreated group.
31525200	5	51	theme	essential	640:648	arg1	oils					650:653	officinalis essential oils	628:653	officinalis essential oils	628:653	GC/MS analysis of M. alternifolia and R. officinalis essential oils revealed richness in oxygenated monoterpenes, representing 51.06% and 69.61% of the total oil composition, respectively.
31525200	1	52	theme	wound	184:188	arg1	healing					190:196	wound healing	184:196	the wound healing potential of three chitosan-based topical preparations loaded with either tea tree essential oil, rosemary essential oil or a mixture of both oils in vivo	180:351	The present study investigates the wound healing potential of three chitosan-based topical preparations loaded with either tea tree essential oil, rosemary essential oil or a mixture of both oils in vivo.
31525200	8	53	theme	oxygenated	1247:1256	arg1	monoterpenes					1258:1269	oxygenated monoterpenes	1247:1269	oxygenated monoterpenes	1247:1269	The high percentage of oxygenated monoterpenes in both essential oils play an important role in the antioxidant and wound healing potential observed herein.
31525200	7	54	theme	combination	1127:1137	arg1	application					1086:1096	topical application	1078:1096	topical application of tea tree and rosemary oil combination	1078:1137	Histopathological examination revealed that topical application of tea tree and rosemary oil combination demonstrated complete re-epithelialization associated with activated hair follicles.
31525200	6	55	theme	formulation	814:824	arg1	application					784:794	Topical application	776:794	Topical application of chitosan-based formulation loaded with a mixture of tea tree and rosemary oils	776:876	Topical application of chitosan-based formulation loaded with a mixture of tea tree and rosemary oils resulted in a significant increase in wound contraction percentage compared to either group treated with individual essential oils and the untreated group.
31525200	7	56	theme	rosemary	1114:1121	arg1	combination					1127:1137	rosemary oil combination	1114:1137	rosemary oil combination	1114:1137	Histopathological examination revealed that topical application of tea tree and rosemary oil combination demonstrated complete re-epithelialization associated with activated hair follicles.
31525200	8	57	theme	essential	1279:1287	arg1	oils					1289:1292	both essential oils	1274:1292	both essential oils	1274:1292	The high percentage of oxygenated monoterpenes in both essential oils play an important role in the antioxidant and wound healing potential observed herein.
31525200	6	58	theme	essential	994:1002	arg1	oils					1004:1007	individual essential oils	983:1007	individual essential oils	983:1007	Topical application of chitosan-based formulation loaded with a mixture of tea tree and rosemary oils resulted in a significant increase in wound contraction percentage compared to either group treated with individual essential oils and the untreated group.
31525200	8	59	theme	wound	1340:1344	arg1	healing					1346:1352	wound healing	1340:1352	wound healing	1340:1352	The high percentage of oxygenated monoterpenes in both essential oils play an important role in the antioxidant and wound healing potential observed herein.
31525200	7	60	theme	tree	1105:1108	arg1	application					1086:1096	topical application	1078:1096	topical application of tea tree and rosemary oil combination	1078:1137	Histopathological examination revealed that topical application of tea tree and rosemary oil combination demonstrated complete re-epithelialization associated with activated hair follicles.
31525200	1	61	theme	topical	232:238	arg1	preparations					240:251	three chitosan-based topical preparations	211:251	three chitosan-based topical preparations loaded with either tea tree essential oil, rosemary essential oil or a mixture of both oils in vivo	211:351	The present study investigates the wound healing potential of three chitosan-based topical preparations loaded with either tea tree essential oil, rosemary essential oil or a mixture of both oils in vivo.
31525200	5	62	theme	total	739:743	arg1	composition					749:759	the total oil composition	735:759	the total oil composition	735:759	GC/MS analysis of M. alternifolia and R. officinalis essential oils revealed richness in oxygenated monoterpenes, representing 51.06% and 69.61% of the total oil composition, respectively.
31525200	8	63	theme	important	1302:1310	arg1	role					1312:1315	an important role	1299:1315	an important role	1299:1315	The high percentage of oxygenated monoterpenes in both essential oils play an important role in the antioxidant and wound healing potential observed herein.
31525200	7	64	theme	topical	1078:1084	arg1	application					1086:1096	topical application	1078:1096	topical application of tea tree and rosemary oil combination	1078:1137	Histopathological examination revealed that topical application of tea tree and rosemary oil combination demonstrated complete re-epithelialization associated with activated hair follicles.
31525200	1	65	theme	present	153:159	arg1	study					161:165	The present study	149:165	The present study	149:165	The present study investigates the wound healing potential of three chitosan-based topical preparations loaded with either tea tree essential oil, rosemary essential oil or a mixture of both oils in vivo.
31525200	5	66	theme	oil	745:747	arg1	composition					749:759	the total oil composition	735:759	the total oil composition	735:759	GC/MS analysis of M. alternifolia and R. officinalis essential oils revealed richness in oxygenated monoterpenes, representing 51.06% and 69.61% of the total oil composition, respectively.
31525200	7	67	theme	oil	1123:1125	arg1	combination					1127:1137	rosemary oil combination	1114:1137	rosemary oil combination	1114:1137	Histopathological examination revealed that topical application of tea tree and rosemary oil combination demonstrated complete re-epithelialization associated with activated hair follicles.
31525200	0	68	theme	essential	97:105	arg1	preparations					135:146	essential oil-loaded chitosan topical preparations	97:146	essential oil-loaded chitosan topical preparations	97:146	Appraisal on the wound healing potential of Melaleuca alternifolia and Rosmarinus officinalis L. essential oil-loaded chitosan topical preparations.
31525200	9	69	from	preparations	1453:1464	arg1	combination					1481:1491	appropriate combination	1469:1491	appropriate combination	1469:1491	Incorporation of tea tree and rosemary essential oils in chitosan-based preparations in appropriate combination could efficiently promote different stages of wound healing.
31525200	6	70	theme	untreated	1017:1025	arg1	group					1027:1031	the untreated group	1013:1031	the untreated group	1013:1031	Topical application of chitosan-based formulation loaded with a mixture of tea tree and rosemary oils resulted in a significant increase in wound contraction percentage compared to either group treated with individual essential oils and the untreated group.
31525200	4	71	theme	Wound	502:506	arg1	healing					508:514	Wound healing	502:514	Wound healing potential	502:524	Wound healing potential was evaluated in vivo using an excision wound model in rats.
31525200	0	72	theme	chitosan	118:125	arg1	preparations					135:146	essential oil-loaded chitosan topical preparations	97:146	essential oil-loaded chitosan topical preparations	97:146	Appraisal on the wound healing potential of Melaleuca alternifolia and Rosmarinus officinalis L. essential oil-loaded chitosan topical preparations.
31525200	9	73	theme	tree	1402:1405	arg1	Incorporation					1381:1393	Incorporation	1381:1393	Incorporation of tea tree and rosemary essential oils in chitosan-based preparations in appropriate combination	1381:1491	Incorporation of tea tree and rosemary essential oils in chitosan-based preparations in appropriate combination could efficiently promote different stages of wound healing.
31525200	5	74	theme	officinalis	628:638	arg1	oils					650:653	officinalis essential oils	628:653	officinalis essential oils	628:653	GC/MS analysis of M. alternifolia and R. officinalis essential oils revealed richness in oxygenated monoterpenes, representing 51.06% and 69.61% of the total oil composition, respectively.
31525200	5	75	theme	composition	749:759	arg1	%					719:719	51.06%	714:719	51.06%	714:719	GC/MS analysis of M. alternifolia and R. officinalis essential oils revealed richness in oxygenated monoterpenes, representing 51.06% and 69.61% of the total oil composition, respectively.
31525200	5	75	theme	composition	749:759	arg1	%					730:730	69.61%	725:730	69.61% of the total oil composition	725:759	GC/MS analysis of M. alternifolia and R. officinalis essential oils revealed richness in oxygenated monoterpenes, representing 51.06% and 69.61% of the total oil composition, respectively.
31525200	5	75	theme	composition	749:759	arg1	composition					749:759	the total oil composition	735:759	the total oil composition	735:759	GC/MS analysis of M. alternifolia and R. officinalis essential oils revealed richness in oxygenated monoterpenes, representing 51.06% and 69.61% of the total oil composition, respectively.
31525200	9	76	theme	rosemary	1411:1418	arg1	oils					1430:1433	rosemary essential oils	1411:1433	rosemary essential oils	1411:1433	Incorporation of tea tree and rosemary essential oils in chitosan-based preparations in appropriate combination could efficiently promote different stages of wound healing.
31525200	6	77	theme	tea	851:853	arg1	tree					855:858	tea tree	851:858	tea tree	851:858	Topical application of chitosan-based formulation loaded with a mixture of tea tree and rosemary oils resulted in a significant increase in wound contraction percentage compared to either group treated with individual essential oils and the untreated group.
31525200	2	78	theme	alternifolia	375:386	arg1	oils					364:367	Essential oils	354:367	Essential oils of M. alternifolia and R. officinalis	354:405	Essential oils of M. alternifolia and R. officinalis were analyzed using GC/MS.
31525200	0	79	theme	wound	17:21	arg1	healing					23:29	wound healing	17:29	the wound healing potential of Melaleuca alternifolia and Rosmarinus officinalis L. essential oil-loaded chitosan topical preparations	13:146	Appraisal on the wound healing potential of Melaleuca alternifolia and Rosmarinus officinalis L. essential oil-loaded chitosan topical preparations.
31525200	9	80	theme	oils	1430:1433	arg1	Incorporation					1381:1393	Incorporation	1381:1393	Incorporation of tea tree and rosemary essential oils in chitosan-based preparations in appropriate combination	1381:1491	Incorporation of tea tree and rosemary essential oils in chitosan-based preparations in appropriate combination could efficiently promote different stages of wound healing.
31525200	9	81	theme	chitosan-based	1438:1451	arg1	preparations					1453:1464	chitosan-based preparations	1438:1464	chitosan-based preparations in appropriate combination	1438:1491	Incorporation of tea tree and rosemary essential oils in chitosan-based preparations in appropriate combination could efficiently promote different stages of wound healing.
31525200	3	82	theme	oil-loaded	444:453	arg1	preparations					472:483	Essential oil-loaded chitosan topical preparations	434:483	Essential oil-loaded chitosan topical preparations	434:483	Essential oil-loaded chitosan topical preparations were formulated.
31525200	6	83	dep	resulted	878:885	arg1	compared					945:952	compared	945:952	compared to either group treated with individual essential oils and the untreated group	945:1031	Topical application of chitosan-based formulation loaded with a mixture of tea tree and rosemary oils resulted in a significant increase in wound contraction percentage compared to either group treated with individual essential oils and the untreated group.
31525200	6	84	theme	oils	873:876	arg1	mixture					840:846	a mixture	838:846	a mixture of tea tree and rosemary oils	838:876	Topical application of chitosan-based formulation loaded with a mixture of tea tree and rosemary oils resulted in a significant increase in wound contraction percentage compared to either group treated with individual essential oils and the untreated group.
31525200	4	85	theme	wound	566:570	arg1	model					572:576	an excision wound model	554:576	an excision wound model in rats	554:584	Wound healing potential was evaluated in vivo using an excision wound model in rats.
31525200	8	86	from	percentage	1233:1242	arg1	oils					1289:1292	both essential oils	1274:1292	both essential oils	1274:1292	The high percentage of oxygenated monoterpenes in both essential oils play an important role in the antioxidant and wound healing potential observed herein.
31525200	3	87	theme	chitosan	455:462	arg1	preparations					472:483	Essential oil-loaded chitosan topical preparations	434:483	Essential oil-loaded chitosan topical preparations	434:483	Essential oil-loaded chitosan topical preparations were formulated.
31525200	1	88	theme	tea	272:274	arg1	oil					291:293	tea tree essential oil	272:293	tea tree essential oil	272:293	The present study investigates the wound healing potential of three chitosan-based topical preparations loaded with either tea tree essential oil, rosemary essential oil or a mixture of both oils in vivo.
31525200	9	89	theme	different	1519:1527	arg1	stages					1529:1534	different stages	1519:1534	different stages of wound healing	1519:1551	Incorporation of tea tree and rosemary essential oils in chitosan-based preparations in appropriate combination could efficiently promote different stages of wound healing.
30200589	9	0	theme	degradation	1287:1297	arg1	degree					1277:1282	the highest degree	1265:1282	the highest degree of degradation of the total fructan content	1265:1326	Overall, Saccharomycescerevisiae isolated from Austrian traditional sourdough showed the highest degree of degradation of the total fructan content and the highest gas building capacity, followed by Torulasporadelbrueckii.
30200589	9	0	theme	degradation	1287:1297	arg1	capacity					1357:1364	the highest gas building capacity	1332:1364	the highest gas building capacity	1332:1364	Overall, Saccharomycescerevisiae isolated from Austrian traditional sourdough showed the highest degree of degradation of the total fructan content and the highest gas building capacity, followed by Torulasporadelbrueckii.
30200589	10	1	theme	yeast	1477:1481	arg1	strains					1483:1489	yeast strains	1477:1489	yeast strains	1477:1489	Hence, this study provides novel knowledge about the FODMAP conversion of yeast strains.
30200589	9	2	theme	building	1348:1355	arg1	capacity					1357:1364	the highest gas building capacity	1332:1364	the highest gas building capacity	1332:1364	Overall, Saccharomycescerevisiae isolated from Austrian traditional sourdough showed the highest degree of degradation of the total fructan content and the highest gas building capacity, followed by Torulasporadelbrueckii.
30200589	9	3	theme	gas	1344:1346	arg1	capacity					1357:1364	the highest gas building capacity	1332:1364	the highest gas building capacity	1332:1364	Overall, Saccharomycescerevisiae isolated from Austrian traditional sourdough showed the highest degree of degradation of the total fructan content and the highest gas building capacity, followed by Torulasporadelbrueckii.
30200589	8	4	theme	examined	1085:1092	arg1	strains					1100:1106	examined yeast strains	1085:1106	the examined yeast strains' ability to degrade fructans	1081:1135	The results revealed strong differences in the examined yeast strains' ability to degrade fructans, in both the model system and wheat flour.
30200589	1	5	from	polyols	154:160	arg1	low					81:83	low	81:83	low	81:83	A diet low in fermentable oligosaccharides, disaccharides, monosaccharides and, polyols (FODMAPs) is recommended for people affected by irritable bowel syndrome (IBS) and non-coeliac wheat sensitivity (NCWS) in order to reduce symptoms.
30200589	3	6	theme	typical	481:487	arg1	profile					508:514	a typical wheat carbohydrate profile	479:514	a typical wheat carbohydrate profile	479:514	First, a model system containing a typical wheat carbohydrate profile was applied to evaluate the growth rate of each yeast strain.
30200589	5	7	used	used	778:781	arg2	approach					735:742	A more realistic approach	718:742	A more realistic approach with a wheat flour suspension	718:772	A more realistic approach with a wheat flour suspension was used to characterize CO₂ production according to the Einhorn method.
30200589	6	8	theme	enzymatic	905:913	arg1	method					915:920	an enzymatic method	902:920	an enzymatic method	902:920	The reduction of the total fructans was analyzed using an enzymatic method.
30200589	9	9	theme	Austrian	1227:1234	arg1	sourdough					1248:1256	Austrian traditional sourdough	1227:1256	Austrian traditional sourdough	1227:1256	Overall, Saccharomycescerevisiae isolated from Austrian traditional sourdough showed the highest degree of degradation of the total fructan content and the highest gas building capacity, followed by Torulasporadelbrueckii.
30200589	4	10	theme	anion-exchange	679:692	arg1	chromatography					694:707	high-pressure anion-exchange chromatography	665:707	high-pressure anion-exchange chromatography (HPAEC)	665:715	Additionally, changes in the sugar composition, for up to four days, were monitored by high-pressure anion-exchange chromatography (HPAEC).
30200589	4	10	theme	anion-exchange	679:692	arg1	HPAEC					710:714	HPAEC	710:714	HPAEC	710:714	Additionally, changes in the sugar composition, for up to four days, were monitored by high-pressure anion-exchange chromatography (HPAEC).
30200589	1	11	from	monosaccharides	133:147	arg1	low					81:83	low	81:83	low	81:83	A diet low in fermentable oligosaccharides, disaccharides, monosaccharides and, polyols (FODMAPs) is recommended for people affected by irritable bowel syndrome (IBS) and non-coeliac wheat sensitivity (NCWS) in order to reduce symptoms.
30200589	3	12	theme	wheat	489:493	arg1	profile					508:514	a typical wheat carbohydrate profile	479:514	a typical wheat carbohydrate profile	479:514	First, a model system containing a typical wheat carbohydrate profile was applied to evaluate the growth rate of each yeast strain.
30200589	4	13	dep	four	636:639	arg1	to					633:634	to	633:634	to	633:634	Additionally, changes in the sugar composition, for up to four days, were monitored by high-pressure anion-exchange chromatography (HPAEC).
30200589	1	14	dep	order	285:289	arg1	reduce					294:299	reduce	294:299	to reduce symptoms	291:308	A diet low in fermentable oligosaccharides, disaccharides, monosaccharides and, polyols (FODMAPs) is recommended for people affected by irritable bowel syndrome (IBS) and non-coeliac wheat sensitivity (NCWS) in order to reduce symptoms.
30200589	8	15	theme	wheat	1167:1171	arg1	flour					1173:1177	wheat flour	1167:1177	wheat flour	1167:1177	The results revealed strong differences in the examined yeast strains' ability to degrade fructans, in both the model system and wheat flour.
30200589	10	16	theme	strains	1483:1489	arg1	conversion					1463:1472	the FODMAP conversion	1452:1472	the FODMAP conversion of yeast strains	1452:1489	Hence, this study provides novel knowledge about the FODMAP conversion of yeast strains.
30200589	8	17	theme	model	1150:1154	arg1	system					1156:1161	the model system	1146:1161	the model system	1146:1161	The results revealed strong differences in the examined yeast strains' ability to degrade fructans, in both the model system and wheat flour.
30200589	7	18	with	fingerprint	938:948	arg1	degrees					989:995	different degrees	979:995	different degrees of polymerization	979:1013	Furthermore, a fingerprint of the present fructans with different degrees of polymerization was analyzed by HPAEC.
30200589	9	19	theme	highest	1336:1342	arg1	capacity					1357:1364	the highest gas building capacity	1332:1364	the highest gas building capacity	1332:1364	Overall, Saccharomycescerevisiae isolated from Austrian traditional sourdough showed the highest degree of degradation of the total fructan content and the highest gas building capacity, followed by Torulasporadelbrueckii.
30200589	1	20	from	disaccharides	118:130	arg1	low					81:83	low	81:83	low	81:83	A diet low in fermentable oligosaccharides, disaccharides, monosaccharides and, polyols (FODMAPs) is recommended for people affected by irritable bowel syndrome (IBS) and non-coeliac wheat sensitivity (NCWS) in order to reduce symptoms.
30200589	9	21	theme	content	1320:1326	arg1	degradation					1287:1297	degradation	1287:1297	degradation of the total fructan content	1287:1326	Overall, Saccharomycescerevisiae isolated from Austrian traditional sourdough showed the highest degree of degradation of the total fructan content and the highest gas building capacity, followed by Torulasporadelbrueckii.
30200589	2	22	theme	study	338:342	arg1	aim					326:328	the aim	322:328	the aim of this study	322:342	Therefore, the aim of this study was to evaluate the impact of 13 sourdough-related yeasts on FODMAP degradation, especially fructans.
30200589	2	23	theme	yeasts	395:400	arg1	impact					364:369	the impact	360:369	the impact of 13 sourdough-related yeasts on FODMAP degradation	360:422	Therefore, the aim of this study was to evaluate the impact of 13 sourdough-related yeasts on FODMAP degradation, especially fructans.
30200589	7	24	theme	polymerization	1000:1013	arg1	degrees					989:995	different degrees	979:995	different degrees of polymerization	979:1013	Furthermore, a fingerprint of the present fructans with different degrees of polymerization was analyzed by HPAEC.
30200589	4	25	from	changes	592:598	arg1	composition					613:623	the sugar composition	603:623	the sugar composition	603:623	Additionally, changes in the sugar composition, for up to four days, were monitored by high-pressure anion-exchange chromatography (HPAEC).
30200589	4	26	theme	sugar	607:611	arg1	composition					613:623	the sugar composition	603:623	the sugar composition	603:623	Additionally, changes in the sugar composition, for up to four days, were monitored by high-pressure anion-exchange chromatography (HPAEC).
30200589	2	27	theme	sourdough-related	377:393	arg1	yeasts					395:400	13 sourdough-related yeasts	374:400	13 sourdough-related yeasts	374:400	Therefore, the aim of this study was to evaluate the impact of 13 sourdough-related yeasts on FODMAP degradation, especially fructans.
30200589	1	28	theme	non-coeliac	245:255	arg1	sensitivity					263:273	non-coeliac wheat sensitivity	245:273	non-coeliac wheat sensitivity (NCWS)	245:280	A diet low in fermentable oligosaccharides, disaccharides, monosaccharides and, polyols (FODMAPs) is recommended for people affected by irritable bowel syndrome (IBS) and non-coeliac wheat sensitivity (NCWS) in order to reduce symptoms.
30200589	1	28	theme	non-coeliac	245:255	arg1	NCWS					276:279	NCWS	276:279	NCWS	276:279	A diet low in fermentable oligosaccharides, disaccharides, monosaccharides and, polyols (FODMAPs) is recommended for people affected by irritable bowel syndrome (IBS) and non-coeliac wheat sensitivity (NCWS) in order to reduce symptoms.
30200589	1	29	from	oligosaccharides	100:115	arg1	low					81:83	low	81:83	low	81:83	A diet low in fermentable oligosaccharides, disaccharides, monosaccharides and, polyols (FODMAPs) is recommended for people affected by irritable bowel syndrome (IBS) and non-coeliac wheat sensitivity (NCWS) in order to reduce symptoms.
30200589	1	30	theme	low	81:83	arg1	diet					76:79	A diet	74:79	A diet low in fermentable oligosaccharides, disaccharides, monosaccharides and, polyols (FODMAPs)	74:170	A diet low in fermentable oligosaccharides, disaccharides, monosaccharides and, polyols (FODMAPs) is recommended for people affected by irritable bowel syndrome (IBS) and non-coeliac wheat sensitivity (NCWS) in order to reduce symptoms.
30200589	5	31	theme	CO₂	799:801	arg1	production					803:812	CO₂ production	799:812	CO₂ production	799:812	A more realistic approach with a wheat flour suspension was used to characterize CO₂ production according to the Einhorn method.
30200589	0	32	theme	Fermentation	23:34	arg1	Applicability					0:12	Applicability	0:12	Applicability of Yeast Fermentation to	0:37	Applicability of Yeast Fermentation to Reduce Fructans and Other FODMAPs.
30200589	1	33	theme	wheat	257:261	arg1	sensitivity					263:273	non-coeliac wheat sensitivity	245:273	non-coeliac wheat sensitivity (NCWS)	245:280	A diet low in fermentable oligosaccharides, disaccharides, monosaccharides and, polyols (FODMAPs) is recommended for people affected by irritable bowel syndrome (IBS) and non-coeliac wheat sensitivity (NCWS) in order to reduce symptoms.
30200589	1	33	theme	wheat	257:261	arg1	NCWS					276:279	NCWS	276:279	NCWS	276:279	A diet low in fermentable oligosaccharides, disaccharides, monosaccharides and, polyols (FODMAPs) is recommended for people affected by irritable bowel syndrome (IBS) and non-coeliac wheat sensitivity (NCWS) in order to reduce symptoms.
30200589	5	34	with	approach	735:742	arg1	suspension					763:772	a wheat flour suspension	749:772	a wheat flour suspension	749:772	A more realistic approach with a wheat flour suspension was used to characterize CO₂ production according to the Einhorn method.
30200589	3	35	theme	carbohydrate	495:506	arg1	profile					508:514	a typical wheat carbohydrate profile	479:514	a typical wheat carbohydrate profile	479:514	First, a model system containing a typical wheat carbohydrate profile was applied to evaluate the growth rate of each yeast strain.
30200589	5	36	theme	wheat	751:755	arg1	suspension					763:772	a wheat flour suspension	749:772	a wheat flour suspension	749:772	A more realistic approach with a wheat flour suspension was used to characterize CO₂ production according to the Einhorn method.
30200589	0	37	theme	Yeast	17:21	arg1	Fermentation					23:34	Yeast Fermentation	17:34	Yeast Fermentation	17:34	Applicability of Yeast Fermentation to Reduce Fructans and Other FODMAPs.
30200589	10	38	theme	novel	1430:1434	arg1	knowledge					1436:1444	novel knowledge	1430:1444	novel knowledge about the FODMAP conversion of yeast strains	1430:1489	Hence, this study provides novel knowledge about the FODMAP conversion of yeast strains.
30200589	1	39	theme	fermentable	88:98	arg1	oligosaccharides					100:115	fermentable oligosaccharides	88:115	fermentable oligosaccharides	88:115	A diet low in fermentable oligosaccharides, disaccharides, monosaccharides and, polyols (FODMAPs) is recommended for people affected by irritable bowel syndrome (IBS) and non-coeliac wheat sensitivity (NCWS) in order to reduce symptoms.
30200589	1	39	theme	fermentable	88:98	arg1	FODMAPs					163:169	FODMAPs	163:169	FODMAPs	163:169	A diet low in fermentable oligosaccharides, disaccharides, monosaccharides and, polyols (FODMAPs) is recommended for people affected by irritable bowel syndrome (IBS) and non-coeliac wheat sensitivity (NCWS) in order to reduce symptoms.
30200589	5	40	theme	flour	757:761	arg1	suspension					763:772	a wheat flour suspension	749:772	a wheat flour suspension	749:772	A more realistic approach with a wheat flour suspension was used to characterize CO₂ production according to the Einhorn method.
30200589	6	41	theme	fructans	874:881	arg1	reduction					851:859	The reduction	847:859	The reduction of the total fructans	847:881	The reduction of the total fructans was analyzed using an enzymatic method.
30200589	9	42	theme	traditional	1236:1246	arg1	sourdough					1248:1256	Austrian traditional sourdough	1227:1256	Austrian traditional sourdough	1227:1256	Overall, Saccharomycescerevisiae isolated from Austrian traditional sourdough showed the highest degree of degradation of the total fructan content and the highest gas building capacity, followed by Torulasporadelbrueckii.
30200589	10	43	theme	FODMAP	1456:1461	arg1	conversion					1463:1472	the FODMAP conversion	1452:1472	the FODMAP conversion of yeast strains	1452:1489	Hence, this study provides novel knowledge about the FODMAP conversion of yeast strains.
30200589	9	44	attach	isolated	1213:1220	arg1	sourdough					1248:1256	Austrian traditional sourdough	1227:1256	Austrian traditional sourdough	1227:1256	Overall, Saccharomycescerevisiae isolated from Austrian traditional sourdough showed the highest degree of degradation of the total fructan content and the highest gas building capacity, followed by Torulasporadelbrueckii.
30200589	9	44	attach	isolated	1213:1220	arg2	Saccharomycescerevisiae					1189:1211	Saccharomycescerevisiae	1189:1211	Saccharomycescerevisiae isolated from Austrian traditional sourdough	1189:1256	Overall, Saccharomycescerevisiae isolated from Austrian traditional sourdough showed the highest degree of degradation of the total fructan content and the highest gas building capacity, followed by Torulasporadelbrueckii.
30200589	6	45	theme	total	868:872	arg1	fructans					874:881	the total fructans	864:881	the total fructans	864:881	The reduction of the total fructans was analyzed using an enzymatic method.
30200589	7	46	theme	different	979:987	arg1	degrees					989:995	different degrees	979:995	different degrees of polymerization	979:1013	Furthermore, a fingerprint of the present fructans with different degrees of polymerization was analyzed by HPAEC.
30200589	3	47	theme	strain	570:575	arg1	rate					551:554	the growth rate	540:554	the growth rate of each yeast strain	540:575	First, a model system containing a typical wheat carbohydrate profile was applied to evaluate the growth rate of each yeast strain.
30200589	3	48	theme	growth	544:549	arg1	rate					551:554	the growth rate	540:554	the growth rate of each yeast strain	540:575	First, a model system containing a typical wheat carbohydrate profile was applied to evaluate the growth rate of each yeast strain.
30200589	8	49	theme	yeast	1094:1098	arg1	strains					1100:1106	examined yeast strains	1085:1106	the examined yeast strains' ability to degrade fructans	1081:1135	The results revealed strong differences in the examined yeast strains' ability to degrade fructans, in both the model system and wheat flour.
30200589	2	50	theme	FODMAP	405:410	arg1	degradation					412:422	FODMAP degradation	405:422	FODMAP degradation	405:422	Therefore, the aim of this study was to evaluate the impact of 13 sourdough-related yeasts on FODMAP degradation, especially fructans.
30200589	3	51	contain	containing	468:477	arg2	profile					508:514	a typical wheat carbohydrate profile	479:514	a typical wheat carbohydrate profile	479:514	First, a model system containing a typical wheat carbohydrate profile was applied to evaluate the growth rate of each yeast strain.
30200589	3	51	contain	containing	468:477	arg1	system					461:466	a model system	453:466	a model system containing a typical wheat carbohydrate profile	453:514	First, a model system containing a typical wheat carbohydrate profile was applied to evaluate the growth rate of each yeast strain.
30200589	7	52	theme	present	957:963	arg1	fructans					965:972	the present fructans	953:972	the present fructans	953:972	Furthermore, a fingerprint of the present fructans with different degrees of polymerization was analyzed by HPAEC.
30200589	9	53	theme	highest	1269:1275	arg1	degree					1277:1282	the highest degree	1265:1282	the highest degree of degradation of the total fructan content	1265:1326	Overall, Saccharomycescerevisiae isolated from Austrian traditional sourdough showed the highest degree of degradation of the total fructan content and the highest gas building capacity, followed by Torulasporadelbrueckii.
30200589	5	54	theme	Einhorn	831:837	arg1	method					839:844	the Einhorn method	827:844	the Einhorn method	827:844	A more realistic approach with a wheat flour suspension was used to characterize CO₂ production according to the Einhorn method.
30200589	7	55	theme	fructans	965:972	arg1	fingerprint					938:948	a fingerprint	936:948	a fingerprint of the present fructans with different degrees of polymerization	936:1013	Furthermore, a fingerprint of the present fructans with different degrees of polymerization was analyzed by HPAEC.
30200589	9	56	theme	total	1306:1310	arg1	content					1320:1326	the total fructan content	1302:1326	the total fructan content	1302:1326	Overall, Saccharomycescerevisiae isolated from Austrian traditional sourdough showed the highest degree of degradation of the total fructan content and the highest gas building capacity, followed by Torulasporadelbrueckii.
30200589	4	57	theme	high-pressure	665:677	arg1	chromatography					694:707	high-pressure anion-exchange chromatography	665:707	high-pressure anion-exchange chromatography (HPAEC)	665:715	Additionally, changes in the sugar composition, for up to four days, were monitored by high-pressure anion-exchange chromatography (HPAEC).
30200589	4	57	theme	high-pressure	665:677	arg1	HPAEC					710:714	HPAEC	710:714	HPAEC	710:714	Additionally, changes in the sugar composition, for up to four days, were monitored by high-pressure anion-exchange chromatography (HPAEC).
30200589	9	58	theme	fructan	1312:1318	arg1	content					1320:1326	the total fructan content	1302:1326	the total fructan content	1302:1326	Overall, Saccharomycescerevisiae isolated from Austrian traditional sourdough showed the highest degree of degradation of the total fructan content and the highest gas building capacity, followed by Torulasporadelbrueckii.
30200589	3	59	theme	yeast	564:568	arg1	strain					570:575	each yeast strain	559:575	each yeast strain	559:575	First, a model system containing a typical wheat carbohydrate profile was applied to evaluate the growth rate of each yeast strain.
30200589	1	60	theme	irritable	210:218	arg1	IBS					236:238	IBS	236:238	IBS	236:238	A diet low in fermentable oligosaccharides, disaccharides, monosaccharides and, polyols (FODMAPs) is recommended for people affected by irritable bowel syndrome (IBS) and non-coeliac wheat sensitivity (NCWS) in order to reduce symptoms.
30200589	1	60	theme	irritable	210:218	arg1	syndrome					226:233	irritable bowel syndrome	210:233	irritable bowel syndrome (IBS)	210:239	A diet low in fermentable oligosaccharides, disaccharides, monosaccharides and, polyols (FODMAPs) is recommended for people affected by irritable bowel syndrome (IBS) and non-coeliac wheat sensitivity (NCWS) in order to reduce symptoms.
30200589	8	61	theme	strong	1059:1064	arg1	differences					1066:1076	strong differences	1059:1076	strong differences in the examined yeast strains' ability to degrade fructans	1059:1135	The results revealed strong differences in the examined yeast strains' ability to degrade fructans, in both the model system and wheat flour.
30200589	3	62	theme	model	455:459	arg1	system					461:466	a model system	453:466	a model system containing a typical wheat carbohydrate profile	453:514	First, a model system containing a typical wheat carbohydrate profile was applied to evaluate the growth rate of each yeast strain.
30200589	1	63	from	low	81:83	arg1	disaccharides					118:130	disaccharides	118:130	disaccharides	118:130	A diet low in fermentable oligosaccharides, disaccharides, monosaccharides and, polyols (FODMAPs) is recommended for people affected by irritable bowel syndrome (IBS) and non-coeliac wheat sensitivity (NCWS) in order to reduce symptoms.
30200589	1	63	from	low	81:83	arg1	oligosaccharides					100:115	fermentable oligosaccharides	88:115	fermentable oligosaccharides	88:115	A diet low in fermentable oligosaccharides, disaccharides, monosaccharides and, polyols (FODMAPs) is recommended for people affected by irritable bowel syndrome (IBS) and non-coeliac wheat sensitivity (NCWS) in order to reduce symptoms.
30200589	1	63	from	low	81:83	arg1	FODMAPs					163:169	FODMAPs	163:169	FODMAPs	163:169	A diet low in fermentable oligosaccharides, disaccharides, monosaccharides and, polyols (FODMAPs) is recommended for people affected by irritable bowel syndrome (IBS) and non-coeliac wheat sensitivity (NCWS) in order to reduce symptoms.
30200589	1	63	from	low	81:83	arg1	monosaccharides					133:147	monosaccharides	133:147	monosaccharides	133:147	A diet low in fermentable oligosaccharides, disaccharides, monosaccharides and, polyols (FODMAPs) is recommended for people affected by irritable bowel syndrome (IBS) and non-coeliac wheat sensitivity (NCWS) in order to reduce symptoms.
30200589	1	63	from	low	81:83	arg1	polyols					154:160	polyols	154:160	polyols	154:160	A diet low in fermentable oligosaccharides, disaccharides, monosaccharides and, polyols (FODMAPs) is recommended for people affected by irritable bowel syndrome (IBS) and non-coeliac wheat sensitivity (NCWS) in order to reduce symptoms.
30200589	1	64	theme	bowel	220:224	arg1	IBS					236:238	IBS	236:238	IBS	236:238	A diet low in fermentable oligosaccharides, disaccharides, monosaccharides and, polyols (FODMAPs) is recommended for people affected by irritable bowel syndrome (IBS) and non-coeliac wheat sensitivity (NCWS) in order to reduce symptoms.
30200589	1	64	theme	bowel	220:224	arg1	syndrome					226:233	irritable bowel syndrome	210:233	irritable bowel syndrome (IBS)	210:239	A diet low in fermentable oligosaccharides, disaccharides, monosaccharides and, polyols (FODMAPs) is recommended for people affected by irritable bowel syndrome (IBS) and non-coeliac wheat sensitivity (NCWS) in order to reduce symptoms.
30200589	0	65	theme	Other	59:63	arg1	FODMAPs					65:71	Other FODMAPs	59:71	Other FODMAPs	59:71	Applicability of Yeast Fermentation to Reduce Fructans and Other FODMAPs.
30200589	2	66	from	impact	364:369	arg1	degradation					412:422	FODMAP degradation	405:422	FODMAP degradation	405:422	Therefore, the aim of this study was to evaluate the impact of 13 sourdough-related yeasts on FODMAP degradation, especially fructans.
30200589	5	67	theme	realistic	725:733	arg1	approach					735:742	A more realistic approach	718:742	A more realistic approach with a wheat flour suspension	718:772	A more realistic approach with a wheat flour suspension was used to characterize CO₂ production according to the Einhorn method.
29627341	10	0	theme	premature	1760:1768	arg1	babies					1770:1775	premature babies	1760:1775	premature babies	1760:1775	These results indicate that RGC dendrite reorganization induced by neonatal inflammation may contribute to the retinal cell dysfunctions associated with systemic inflammation in premature babies.
29627341	5	1	dep	types	773:777	arg1	II					788:789	II	788:789	II	788:789	Three types (Type I, II, III) of RGCs were distinguished and classified according to the characteristic features in their dendritic field area and dendrite density.
29627341	5	1	dep	types	773:777	arg1	I					785:785	Type I	780:785	Type I	780:785	Three types (Type I, II, III) of RGCs were distinguished and classified according to the characteristic features in their dendritic field area and dendrite density.
29627341	4	2	theme	RGCs	574:577	arg1	changes					563:569	Morphological changes	549:569	Morphological changes of RGCs	549:577	Morphological changes of RGCs were investigated by using 3D neuron reconstruction technique in Thy-1 YFPH transgenic mice at P21, of which a fraction of RGCs selectively expresses the yellow fluorescent protein (YFP).
29627341	10	3	from	inflammation	1744:1755	arg1	babies					1770:1775	premature babies	1760:1775	premature babies	1760:1775	These results indicate that RGC dendrite reorganization induced by neonatal inflammation may contribute to the retinal cell dysfunctions associated with systemic inflammation in premature babies.
29627341	9	4	theme	projections	1505:1515	arg1	pattern					1490:1496	the pattern	1486:1496	the pattern of RGC projections in the dorsal lateral geniculate nucleus of the thalamus (dLGN)	1486:1579	Meanwhile, using anterograde labeling of the retinal cells, we found that neonatal exposure to LPS also did not affect the pattern of RGC projections in the dorsal lateral geniculate nucleus of the thalamus (dLGN).
29627341	2	5	theme	retinal	389:395	arg1	RGCs					413:416	RGCs	413:416	RGCs	413:416	In this study, we set out to explore whether systemic inflammatory stress affects the development of retinal ganglion cells (RGCs).
29627341	2	5	theme	retinal	389:395	arg1	cells					406:410	retinal ganglion cells	389:410	retinal ganglion cells (RGCs)	389:417	In this study, we set out to explore whether systemic inflammatory stress affects the development of retinal ganglion cells (RGCs).
29627341	4	6	theme	Thy-1	644:648	arg1	mice					666:669	Thy-1 YFPH transgenic mice	644:669	Thy-1 YFPH transgenic mice	644:669	Morphological changes of RGCs were investigated by using 3D neuron reconstruction technique in Thy-1 YFPH transgenic mice at P21, of which a fraction of RGCs selectively expresses the yellow fluorescent protein (YFP).
29627341	3	7	theme	postnatal	527:535	arg1	day					537:539	postnatal day 4	527:541	postnatal day 4 (P4)	527:546	Neonatal inflammation was induced by single and systemic injection of lipopolysaccharide (LPS, 1 mg/kg) at postnatal day 4 (P4).
29627341	3	7	theme	postnatal	527:535	arg1	P4					544:545	P4	544:545	P4	544:545	Neonatal inflammation was induced by single and systemic injection of lipopolysaccharide (LPS, 1 mg/kg) at postnatal day 4 (P4).
29627341	4	8	theme	transgenic	655:664	arg1	mice					666:669	Thy-1 YFPH transgenic mice	644:669	Thy-1 YFPH transgenic mice	644:669	Morphological changes of RGCs were investigated by using 3D neuron reconstruction technique in Thy-1 YFPH transgenic mice at P21, of which a fraction of RGCs selectively expresses the yellow fluorescent protein (YFP).
29627341	10	9	theme	RGC	1610:1612	arg1	reorganization					1623:1636	RGC dendrite reorganization	1610:1636	RGC dendrite reorganization induced by neonatal inflammation	1610:1669	These results indicate that RGC dendrite reorganization induced by neonatal inflammation may contribute to the retinal cell dysfunctions associated with systemic inflammation in premature babies.
29627341	4	10	from	technique	631:639	arg1	mice					666:669	Thy-1 YFPH transgenic mice	644:669	Thy-1 YFPH transgenic mice	644:669	Morphological changes of RGCs were investigated by using 3D neuron reconstruction technique in Thy-1 YFPH transgenic mice at P21, of which a fraction of RGCs selectively expresses the yellow fluorescent protein (YFP).
29627341	5	11	theme	RGCs	800:803	arg1	types					773:777	Three types	767:777	Three types (Type I, II, III) of RGCs	767:803	Three types (Type I, II, III) of RGCs were distinguished and classified according to the characteristic features in their dendritic field area and dendrite density.
29627341	9	12	theme	lateral	1531:1537	arg1	nucleus					1550:1556	the dorsal lateral geniculate nucleus	1520:1556	the dorsal lateral geniculate nucleus of the thalamus (dLGN)	1520:1579	Meanwhile, using anterograde labeling of the retinal cells, we found that neonatal exposure to LPS also did not affect the pattern of RGC projections in the dorsal lateral geniculate nucleus of the thalamus (dLGN).
29627341	9	12	theme	lateral	1531:1537	arg1	dLGN					1575:1578	dLGN	1575:1578	dLGN	1575:1578	Meanwhile, using anterograde labeling of the retinal cells, we found that neonatal exposure to LPS also did not affect the pattern of RGC projections in the dorsal lateral geniculate nucleus of the thalamus (dLGN).
29627341	6	13	theme	RGC	1072:1074	arg1	Type					1076:1079	the RGC Type I and II	1068:1088	Type	1076:1079	Neonatal exposure to LPS did not alter the composition of the three RGC types but induced a reorganization of dendritic architecture in the RGC Type I and II (but not Type III).
29627341	4	14	theme	neuron	609:614	arg1	reconstruction					616:629	3D neuron reconstruction	606:629	3D neuron reconstruction technique in Thy-1 YFPH transgenic mice	606:669	Morphological changes of RGCs were investigated by using 3D neuron reconstruction technique in Thy-1 YFPH transgenic mice at P21, of which a fraction of RGCs selectively expresses the yellow fluorescent protein (YFP).
29627341	8	15	theme	RGC	1313:1315	arg1	types					1317:1321	all three RGC types	1303:1321	all three RGC types	1303:1321	However, soma sizes of all three RGC types were not affected by neonatal inflammation.
29627341	9	16	from	pattern	1490:1496	arg1	nucleus					1550:1556	the dorsal lateral geniculate nucleus	1520:1556	the dorsal lateral geniculate nucleus of the thalamus (dLGN)	1520:1579	Meanwhile, using anterograde labeling of the retinal cells, we found that neonatal exposure to LPS also did not affect the pattern of RGC projections in the dorsal lateral geniculate nucleus of the thalamus (dLGN).
29627341	9	16	from	pattern	1490:1496	arg1	dLGN					1575:1578	dLGN	1575:1578	dLGN	1575:1578	Meanwhile, using anterograde labeling of the retinal cells, we found that neonatal exposure to LPS also did not affect the pattern of RGC projections in the dorsal lateral geniculate nucleus of the thalamus (dLGN).
29627341	5	17	theme	characteristic	856:869	arg1	features					871:878	the characteristic features	852:878	the characteristic features in their dendritic field area and dendrite density	852:929	Three types (Type I, II, III) of RGCs were distinguished and classified according to the characteristic features in their dendritic field area and dendrite density.
29627341	7	18	from	diameter	1122:1129	arg1	Type					1181:1184	RGC Type I	1177:1186	RGC Type I	1177:1186	The average diameter, surface area and volume of dendrites in both RGC Type I and II were increased after systemic inflammation compared with those in the control group.
29627341	7	18	from	diameter	1122:1129	arg1	II					1192:1193	II	1192:1193	II	1192:1193	The average diameter, surface area and volume of dendrites in both RGC Type I and II were increased after systemic inflammation compared with those in the control group.
29627341	0	19	theme	retinal	72:78	arg1	cells					89:93	retinal ganglion cells	72:93	retinal ganglion cells	72:93	Neonatal inflammation induces reorganization in dendritic morphology of retinal ganglion cells but not their retinogeniculate projection in mice.
29627341	8	20	theme	neonatal	1344:1351	arg1	inflammation					1353:1364	neonatal inflammation	1344:1364	neonatal inflammation	1344:1364	However, soma sizes of all three RGC types were not affected by neonatal inflammation.
29627341	0	21	theme	cells	89:93	arg1	morphology					58:67	dendritic morphology	48:67	dendritic morphology of retinal ganglion cells	48:93	Neonatal inflammation induces reorganization in dendritic morphology of retinal ganglion cells but not their retinogeniculate projection in mice.
29627341	1	22	theme	preterm	179:185	arg1	babies					187:192	preterm babies	179:192	preterm babies	179:192	Perinatal inflammatory insult in preterm babies is associated with vision impairment, but the underlying cellular mechanism is still unknown.
29627341	6	23	theme	Neonatal	932:939	arg1	exposure					941:948	Neonatal exposure	932:948	Neonatal exposure to LPS	932:955	Neonatal exposure to LPS did not alter the composition of the three RGC types but induced a reorganization of dendritic architecture in the RGC Type I and II (but not Type III).
29627341	4	24	theme	yellow	733:738	arg1	YFP					761:763	YFP	761:763	YFP	761:763	Morphological changes of RGCs were investigated by using 3D neuron reconstruction technique in Thy-1 YFPH transgenic mice at P21, of which a fraction of RGCs selectively expresses the yellow fluorescent protein (YFP).
29627341	4	24	theme	yellow	733:738	arg1	protein					752:758	the yellow fluorescent protein	729:758	the yellow fluorescent protein (YFP)	729:764	Morphological changes of RGCs were investigated by using 3D neuron reconstruction technique in Thy-1 YFPH transgenic mice at P21, of which a fraction of RGCs selectively expresses the yellow fluorescent protein (YFP).
29627341	1	25	theme	Perinatal	146:154	arg1	insult					169:174	Perinatal inflammatory insult	146:174	Perinatal inflammatory insult in preterm babies	146:192	Perinatal inflammatory insult in preterm babies is associated with vision impairment, but the underlying cellular mechanism is still unknown.
29627341	2	26	theme	cells	406:410	arg1	development					374:384	the development	370:384	the development of retinal ganglion cells (RGCs)	370:417	In this study, we set out to explore whether systemic inflammatory stress affects the development of retinal ganglion cells (RGCs).
29627341	0	27	theme	retinogeniculate	109:124	arg1	projection					126:135	their retinogeniculate projection	103:135	reorganization in dendritic morphology of retinal ganglion cells but not their retinogeniculate projection	30:135	Neonatal inflammation induces reorganization in dendritic morphology of retinal ganglion cells but not their retinogeniculate projection in mice.
29627341	9	28	theme	cells	1420:1424	arg1	labeling					1396:1403	anterograde labeling	1384:1403	anterograde labeling of the retinal cells	1384:1424	Meanwhile, using anterograde labeling of the retinal cells, we found that neonatal exposure to LPS also did not affect the pattern of RGC projections in the dorsal lateral geniculate nucleus of the thalamus (dLGN).
29627341	6	29	theme	RGC	1000:1002	arg1	types					1004:1008	the three RGC types	990:1008	the three RGC types	990:1008	Neonatal exposure to LPS did not alter the composition of the three RGC types but induced a reorganization of dendritic architecture in the RGC Type I and II (but not Type III).
29627341	0	30	theme	ganglion	80:87	arg1	cells					89:93	retinal ganglion cells	72:93	retinal ganglion cells	72:93	Neonatal inflammation induces reorganization in dendritic morphology of retinal ganglion cells but not their retinogeniculate projection in mice.
29627341	4	31	theme	RGCs	702:705	arg1	fraction					690:697	a fraction	688:697	a fraction of RGCs	688:705	Morphological changes of RGCs were investigated by using 3D neuron reconstruction technique in Thy-1 YFPH transgenic mice at P21, of which a fraction of RGCs selectively expresses the yellow fluorescent protein (YFP).
29627341	3	32	theme	Neonatal	420:427	arg1	inflammation					429:440	Neonatal inflammation	420:440	Neonatal inflammation	420:440	Neonatal inflammation was induced by single and systemic injection of lipopolysaccharide (LPS, 1 mg/kg) at postnatal day 4 (P4).
29627341	0	33	theme	Neonatal	0:7	arg1	inflammation					9:20	Neonatal inflammation	0:20	Neonatal inflammation	0:20	Neonatal inflammation induces reorganization in dendritic morphology of retinal ganglion cells but not their retinogeniculate projection in mice.
29627341	5	34	theme	Type	780:783	arg1	II					788:789	II	788:789	II	788:789	Three types (Type I, II, III) of RGCs were distinguished and classified according to the characteristic features in their dendritic field area and dendrite density.
29627341	5	34	theme	Type	780:783	arg1	I					785:785	Type I	780:785	Type I	780:785	Three types (Type I, II, III) of RGCs were distinguished and classified according to the characteristic features in their dendritic field area and dendrite density.
29627341	5	35	theme	dendritic	889:897	arg1	area					905:908	their dendritic field area	883:908	their dendritic field area	883:908	Three types (Type I, II, III) of RGCs were distinguished and classified according to the characteristic features in their dendritic field area and dendrite density.
29627341	10	36	theme	cell	1701:1704	arg1	dysfunctions					1706:1717	the retinal cell dysfunctions	1689:1717	the retinal cell dysfunctions associated with systemic inflammation in premature babies	1689:1775	These results indicate that RGC dendrite reorganization induced by neonatal inflammation may contribute to the retinal cell dysfunctions associated with systemic inflammation in premature babies.
29627341	7	37	theme	RGC	1177:1179	arg1	Type					1181:1184	RGC Type I	1177:1186	RGC Type I	1177:1186	The average diameter, surface area and volume of dendrites in both RGC Type I and II were increased after systemic inflammation compared with those in the control group.
29627341	2	38	theme	inflammatory	342:353	arg1	stress					355:360	systemic inflammatory stress	333:360	systemic inflammatory stress	333:360	In this study, we set out to explore whether systemic inflammatory stress affects the development of retinal ganglion cells (RGCs).
29627341	7	39	dep	increased	1200:1208	arg1	compared					1238:1245	compared	1238:1245	compared with those in the control group	1238:1277	The average diameter, surface area and volume of dendrites in both RGC Type I and II were increased after systemic inflammation compared with those in the control group.
29627341	6	40	theme	architecture	1052:1063	arg1	reorganization					1024:1037	a reorganization	1022:1037	a reorganization of dendritic architecture	1022:1063	Neonatal exposure to LPS did not alter the composition of the three RGC types but induced a reorganization of dendritic architecture in the RGC Type I and II (but not Type III).
29627341	10	41	theme	systemic	1735:1742	arg1	inflammation					1744:1755	systemic inflammation	1735:1755	systemic inflammation in premature babies	1735:1775	These results indicate that RGC dendrite reorganization induced by neonatal inflammation may contribute to the retinal cell dysfunctions associated with systemic inflammation in premature babies.
29627341	8	42	theme	soma	1289:1292	arg1	sizes					1294:1298	soma sizes	1289:1298	soma sizes of all three RGC types	1289:1321	However, soma sizes of all three RGC types were not affected by neonatal inflammation.
29627341	1	43	theme	cellular	251:258	arg1	mechanism					260:268	the underlying cellular mechanism	236:268	the underlying cellular mechanism	236:268	Perinatal inflammatory insult in preterm babies is associated with vision impairment, but the underlying cellular mechanism is still unknown.
29627341	1	43	theme	cellular	251:258	arg1	unknown					279:285	unknown	279:285	unknown	279:285	Perinatal inflammatory insult in preterm babies is associated with vision impairment, but the underlying cellular mechanism is still unknown.
29627341	7	44	theme	surface	1132:1138	arg1	area					1140:1143	surface area	1132:1143	surface area	1132:1143	The average diameter, surface area and volume of dendrites in both RGC Type I and II were increased after systemic inflammation compared with those in the control group.
29627341	4	45	theme	Morphological	549:561	arg1	changes					563:569	Morphological changes	549:569	Morphological changes of RGCs	549:577	Morphological changes of RGCs were investigated by using 3D neuron reconstruction technique in Thy-1 YFPH transgenic mice at P21, of which a fraction of RGCs selectively expresses the yellow fluorescent protein (YFP).
29627341	9	46	theme	RGC	1501:1503	arg1	projections					1505:1515	RGC projections	1501:1515	RGC projections in the dorsal lateral geniculate nucleus of the thalamus (dLGN)	1501:1579	Meanwhile, using anterograde labeling of the retinal cells, we found that neonatal exposure to LPS also did not affect the pattern of RGC projections in the dorsal lateral geniculate nucleus of the thalamus (dLGN).
29627341	7	47	from	area	1140:1143	arg1	Type					1181:1184	RGC Type I	1177:1186	RGC Type I	1177:1186	The average diameter, surface area and volume of dendrites in both RGC Type I and II were increased after systemic inflammation compared with those in the control group.
29627341	7	47	from	area	1140:1143	arg1	II					1192:1193	II	1192:1193	II	1192:1193	The average diameter, surface area and volume of dendrites in both RGC Type I and II were increased after systemic inflammation compared with those in the control group.
29627341	3	48	theme	lipopolysaccharide	490:507	arg1	injection					477:485	single and systemic injection	457:485	single and systemic injection of lipopolysaccharide (LPS, 1 mg/kg)	457:522	Neonatal inflammation was induced by single and systemic injection of lipopolysaccharide (LPS, 1 mg/kg) at postnatal day 4 (P4).
29627341	5	49	theme	III	792:794	arg1	II					788:789	II	788:789	II	788:789	Three types (Type I, II, III) of RGCs were distinguished and classified according to the characteristic features in their dendritic field area and dendrite density.
29627341	5	49	theme	III	792:794	arg1	I					785:785	Type I	780:785	Type I	780:785	Three types (Type I, II, III) of RGCs were distinguished and classified according to the characteristic features in their dendritic field area and dendrite density.
29627341	7	50	from	volume	1149:1154	arg1	Type					1181:1184	RGC Type I	1177:1186	RGC Type I	1177:1186	The average diameter, surface area and volume of dendrites in both RGC Type I and II were increased after systemic inflammation compared with those in the control group.
29627341	7	50	from	volume	1149:1154	arg1	II					1192:1193	II	1192:1193	II	1192:1193	The average diameter, surface area and volume of dendrites in both RGC Type I and II were increased after systemic inflammation compared with those in the control group.
29627341	10	51	theme	dendrite	1614:1621	arg1	reorganization					1623:1636	RGC dendrite reorganization	1610:1636	RGC dendrite reorganization induced by neonatal inflammation	1610:1669	These results indicate that RGC dendrite reorganization induced by neonatal inflammation may contribute to the retinal cell dysfunctions associated with systemic inflammation in premature babies.
29627341	9	52	theme	dorsal	1524:1529	arg1	nucleus					1550:1556	the dorsal lateral geniculate nucleus	1520:1556	the dorsal lateral geniculate nucleus of the thalamus (dLGN)	1520:1579	Meanwhile, using anterograde labeling of the retinal cells, we found that neonatal exposure to LPS also did not affect the pattern of RGC projections in the dorsal lateral geniculate nucleus of the thalamus (dLGN).
29627341	9	52	theme	dorsal	1524:1529	arg1	dLGN					1575:1578	dLGN	1575:1578	dLGN	1575:1578	Meanwhile, using anterograde labeling of the retinal cells, we found that neonatal exposure to LPS also did not affect the pattern of RGC projections in the dorsal lateral geniculate nucleus of the thalamus (dLGN).
29627341	4	53	theme	YFPH	650:653	arg1	mice					666:669	Thy-1 YFPH transgenic mice	644:669	Thy-1 YFPH transgenic mice	644:669	Morphological changes of RGCs were investigated by using 3D neuron reconstruction technique in Thy-1 YFPH transgenic mice at P21, of which a fraction of RGCs selectively expresses the yellow fluorescent protein (YFP).
29627341	1	54	theme	vision	213:218	arg1	impairment					220:229	vision impairment	213:229	vision impairment	213:229	Perinatal inflammatory insult in preterm babies is associated with vision impairment, but the underlying cellular mechanism is still unknown.
29627341	9	55	from	projections	1505:1515	arg1	nucleus					1550:1556	the dorsal lateral geniculate nucleus	1520:1556	the dorsal lateral geniculate nucleus of the thalamus (dLGN)	1520:1579	Meanwhile, using anterograde labeling of the retinal cells, we found that neonatal exposure to LPS also did not affect the pattern of RGC projections in the dorsal lateral geniculate nucleus of the thalamus (dLGN).
29627341	9	55	from	projections	1505:1515	arg1	dLGN					1575:1578	dLGN	1575:1578	dLGN	1575:1578	Meanwhile, using anterograde labeling of the retinal cells, we found that neonatal exposure to LPS also did not affect the pattern of RGC projections in the dorsal lateral geniculate nucleus of the thalamus (dLGN).
29627341	9	56	theme	geniculate	1539:1548	arg1	nucleus					1550:1556	the dorsal lateral geniculate nucleus	1520:1556	the dorsal lateral geniculate nucleus of the thalamus (dLGN)	1520:1579	Meanwhile, using anterograde labeling of the retinal cells, we found that neonatal exposure to LPS also did not affect the pattern of RGC projections in the dorsal lateral geniculate nucleus of the thalamus (dLGN).
29627341	9	56	theme	geniculate	1539:1548	arg1	dLGN					1575:1578	dLGN	1575:1578	dLGN	1575:1578	Meanwhile, using anterograde labeling of the retinal cells, we found that neonatal exposure to LPS also did not affect the pattern of RGC projections in the dorsal lateral geniculate nucleus of the thalamus (dLGN).
29627341	4	57	theme	3D	606:607	arg1	reconstruction					616:629	3D neuron reconstruction	606:629	3D neuron reconstruction technique in Thy-1 YFPH transgenic mice	606:669	Morphological changes of RGCs were investigated by using 3D neuron reconstruction technique in Thy-1 YFPH transgenic mice at P21, of which a fraction of RGCs selectively expresses the yellow fluorescent protein (YFP).
29627341	10	58	theme	neonatal	1649:1656	arg1	inflammation					1658:1669	neonatal inflammation	1649:1669	neonatal inflammation	1649:1669	These results indicate that RGC dendrite reorganization induced by neonatal inflammation may contribute to the retinal cell dysfunctions associated with systemic inflammation in premature babies.
29627341	8	59	theme	types	1317:1321	arg1	sizes					1294:1298	soma sizes	1289:1298	soma sizes of all three RGC types	1289:1321	However, soma sizes of all three RGC types were not affected by neonatal inflammation.
29627341	4	60	theme	reconstruction	616:629	arg1	technique					631:639	3D neuron reconstruction technique	606:639	3D neuron reconstruction technique in Thy-1 YFPH transgenic mice	606:669	Morphological changes of RGCs were investigated by using 3D neuron reconstruction technique in Thy-1 YFPH transgenic mice at P21, of which a fraction of RGCs selectively expresses the yellow fluorescent protein (YFP).
29627341	9	61	theme	thalamus	1565:1572	arg1	nucleus					1550:1556	the dorsal lateral geniculate nucleus	1520:1556	the dorsal lateral geniculate nucleus of the thalamus (dLGN)	1520:1579	Meanwhile, using anterograde labeling of the retinal cells, we found that neonatal exposure to LPS also did not affect the pattern of RGC projections in the dorsal lateral geniculate nucleus of the thalamus (dLGN).
29627341	9	61	theme	thalamus	1565:1572	arg1	dLGN					1575:1578	dLGN	1575:1578	dLGN	1575:1578	Meanwhile, using anterograde labeling of the retinal cells, we found that neonatal exposure to LPS also did not affect the pattern of RGC projections in the dorsal lateral geniculate nucleus of the thalamus (dLGN).
29627341	7	62	theme	control	1265:1271	arg1	group					1273:1277	the control group	1261:1277	the control group	1261:1277	The average diameter, surface area and volume of dendrites in both RGC Type I and II were increased after systemic inflammation compared with those in the control group.
29627341	7	63	theme	average	1114:1120	arg1	diameter					1122:1129	The average diameter	1110:1129	The average diameter	1110:1129	The average diameter, surface area and volume of dendrites in both RGC Type I and II were increased after systemic inflammation compared with those in the control group.
29627341	1	64	theme	inflammatory	156:167	arg1	insult					169:174	Perinatal inflammatory insult	146:174	Perinatal inflammatory insult in preterm babies	146:192	Perinatal inflammatory insult in preterm babies is associated with vision impairment, but the underlying cellular mechanism is still unknown.
29627341	0	65	from	reorganization	30:43	arg1	morphology					58:67	dendritic morphology	48:67	dendritic morphology of retinal ganglion cells	48:93	Neonatal inflammation induces reorganization in dendritic morphology of retinal ganglion cells but not their retinogeniculate projection in mice.
29627341	7	66	theme	systemic	1216:1223	arg1	inflammation					1225:1236	systemic inflammation	1216:1236	systemic inflammation	1216:1236	The average diameter, surface area and volume of dendrites in both RGC Type I and II were increased after systemic inflammation compared with those in the control group.
29627341	9	67	theme	anterograde	1384:1394	arg1	labeling					1396:1403	anterograde labeling	1384:1403	anterograde labeling of the retinal cells	1384:1424	Meanwhile, using anterograde labeling of the retinal cells, we found that neonatal exposure to LPS also did not affect the pattern of RGC projections in the dorsal lateral geniculate nucleus of the thalamus (dLGN).
29627341	9	68	from	nucleus	1550:1556	arg1	pattern					1490:1496	the pattern	1486:1496	the pattern of RGC projections in the dorsal lateral geniculate nucleus of the thalamus (dLGN)	1486:1579	Meanwhile, using anterograde labeling of the retinal cells, we found that neonatal exposure to LPS also did not affect the pattern of RGC projections in the dorsal lateral geniculate nucleus of the thalamus (dLGN).
29627341	5	69	theme	field	899:903	arg1	area					905:908	their dendritic field area	883:908	their dendritic field area	883:908	Three types (Type I, II, III) of RGCs were distinguished and classified according to the characteristic features in their dendritic field area and dendrite density.
29627341	1	70	from	insult	169:174	arg1	babies					187:192	preterm babies	179:192	preterm babies	179:192	Perinatal inflammatory insult in preterm babies is associated with vision impairment, but the underlying cellular mechanism is still unknown.
29627341	9	71	theme	retinal	1412:1418	arg1	cells					1420:1424	the retinal cells	1408:1424	the retinal cells	1408:1424	Meanwhile, using anterograde labeling of the retinal cells, we found that neonatal exposure to LPS also did not affect the pattern of RGC projections in the dorsal lateral geniculate nucleus of the thalamus (dLGN).
29627341	6	72	theme	types	1004:1008	arg1	composition					975:985	the composition	971:985	the composition of the three RGC types	971:1008	Neonatal exposure to LPS did not alter the composition of the three RGC types but induced a reorganization of dendritic architecture in the RGC Type I and II (but not Type III).
29627341	4	73	theme	fluorescent	740:750	arg1	YFP					761:763	YFP	761:763	YFP	761:763	Morphological changes of RGCs were investigated by using 3D neuron reconstruction technique in Thy-1 YFPH transgenic mice at P21, of which a fraction of RGCs selectively expresses the yellow fluorescent protein (YFP).
29627341	4	73	theme	fluorescent	740:750	arg1	protein					752:758	the yellow fluorescent protein	729:758	the yellow fluorescent protein (YFP)	729:764	Morphological changes of RGCs were investigated by using 3D neuron reconstruction technique in Thy-1 YFPH transgenic mice at P21, of which a fraction of RGCs selectively expresses the yellow fluorescent protein (YFP).
29627341	2	74	theme	ganglion	397:404	arg1	RGCs					413:416	RGCs	413:416	RGCs	413:416	In this study, we set out to explore whether systemic inflammatory stress affects the development of retinal ganglion cells (RGCs).
29627341	2	74	theme	ganglion	397:404	arg1	cells					406:410	retinal ganglion cells	389:410	retinal ganglion cells (RGCs)	389:417	In this study, we set out to explore whether systemic inflammatory stress affects the development of retinal ganglion cells (RGCs).
29627341	3	75	dep	lipopolysaccharide	490:507	arg1	1 mg/kg					515:521	1 mg/kg	515:521	1 mg/kg	515:521	Neonatal inflammation was induced by single and systemic injection of lipopolysaccharide (LPS, 1 mg/kg) at postnatal day 4 (P4).
29627341	3	75	dep	lipopolysaccharide	490:507	arg1	LPS					510:512	LPS	510:512	LPS	510:512	Neonatal inflammation was induced by single and systemic injection of lipopolysaccharide (LPS, 1 mg/kg) at postnatal day 4 (P4).
29627341	3	76	theme	systemic	468:475	arg1	injection					477:485	single and systemic injection	457:485	single and systemic injection of lipopolysaccharide (LPS, 1 mg/kg)	457:522	Neonatal inflammation was induced by single and systemic injection of lipopolysaccharide (LPS, 1 mg/kg) at postnatal day 4 (P4).
29627341	0	77	theme	dendritic	48:56	arg1	morphology					58:67	dendritic morphology	48:67	dendritic morphology of retinal ganglion cells	48:93	Neonatal inflammation induces reorganization in dendritic morphology of retinal ganglion cells but not their retinogeniculate projection in mice.
29627341	9	78	theme	neonatal	1441:1448	arg1	exposure					1450:1457	neonatal exposure	1441:1457	neonatal exposure to LPS	1441:1464	Meanwhile, using anterograde labeling of the retinal cells, we found that neonatal exposure to LPS also did not affect the pattern of RGC projections in the dorsal lateral geniculate nucleus of the thalamus (dLGN).
29627341	10	79	theme	retinal	1693:1699	arg1	dysfunctions					1706:1717	the retinal cell dysfunctions	1689:1717	the retinal cell dysfunctions associated with systemic inflammation in premature babies	1689:1775	These results indicate that RGC dendrite reorganization induced by neonatal inflammation may contribute to the retinal cell dysfunctions associated with systemic inflammation in premature babies.
29627341	3	80	theme	single	457:462	arg1	injection					477:485	single and systemic injection	457:485	single and systemic injection of lipopolysaccharide (LPS, 1 mg/kg)	457:522	Neonatal inflammation was induced by single and systemic injection of lipopolysaccharide (LPS, 1 mg/kg) at postnatal day 4 (P4).
29627341	7	81	theme	dendrites	1159:1167	arg1	area					1140:1143	surface area	1132:1143	surface area	1132:1143	The average diameter, surface area and volume of dendrites in both RGC Type I and II were increased after systemic inflammation compared with those in the control group.
29627341	7	81	theme	dendrites	1159:1167	arg1	volume					1149:1154	volume	1149:1154	volume of dendrites in both RGC Type I and II	1149:1193	The average diameter, surface area and volume of dendrites in both RGC Type I and II were increased after systemic inflammation compared with those in the control group.
29627341	7	81	theme	dendrites	1159:1167	arg1	diameter					1122:1129	The average diameter	1110:1129	The average diameter	1110:1129	The average diameter, surface area and volume of dendrites in both RGC Type I and II were increased after systemic inflammation compared with those in the control group.
29627341	2	82	theme	systemic	333:340	arg1	stress					355:360	systemic inflammatory stress	333:360	systemic inflammatory stress	333:360	In this study, we set out to explore whether systemic inflammatory stress affects the development of retinal ganglion cells (RGCs).
29627341	6	83	theme	dendritic	1042:1050	arg1	architecture					1052:1063	dendritic architecture	1042:1063	dendritic architecture	1042:1063	Neonatal exposure to LPS did not alter the composition of the three RGC types but induced a reorganization of dendritic architecture in the RGC Type I and II (but not Type III).
29627341	5	84	theme	dendrite	914:921	arg1	density					923:929	dendrite density	914:929	dendrite density	914:929	Three types (Type I, II, III) of RGCs were distinguished and classified according to the characteristic features in their dendritic field area and dendrite density.
29627341	5	85	from	features	871:878	arg1	area					905:908	their dendritic field area	883:908	their dendritic field area	883:908	Three types (Type I, II, III) of RGCs were distinguished and classified according to the characteristic features in their dendritic field area and dendrite density.
29627341	5	85	from	features	871:878	arg1	density					923:929	dendrite density	914:929	dendrite density	914:929	Three types (Type I, II, III) of RGCs were distinguished and classified according to the characteristic features in their dendritic field area and dendrite density.
29627341	1	86	theme	underlying	240:249	arg1	mechanism					260:268	the underlying cellular mechanism	236:268	the underlying cellular mechanism	236:268	Perinatal inflammatory insult in preterm babies is associated with vision impairment, but the underlying cellular mechanism is still unknown.
29627341	1	86	theme	underlying	240:249	arg1	unknown					279:285	unknown	279:285	unknown	279:285	Perinatal inflammatory insult in preterm babies is associated with vision impairment, but the underlying cellular mechanism is still unknown.
29458489	11	0	theme	crystal	1468:1474	arg1	assay					1483:1487	crystal violet assay	1468:1487	crystal violet assay	1468:1487	The strain also showed biofilm production, estimated by using crystal violet assay.
29458489	10	1	theme	whole-cell	1378:1387	arg1	galactose					1359:1367	galactose	1359:1367	galactose	1359:1367	Rhamnose, fucose and galactose were the whole-cell sugars detected.
29458489	10	1	theme	whole-cell	1378:1387	arg1	fucose					1348:1353	fucose	1348:1353	fucose	1348:1353	Rhamnose, fucose and galactose were the whole-cell sugars detected.
29458489	10	1	theme	whole-cell	1378:1387	arg1	Rhamnose					1338:1345	Rhamnose	1338:1345	Rhamnose	1338:1345	Rhamnose, fucose and galactose were the whole-cell sugars detected.
29458489	10	1	theme	whole-cell	1378:1387	arg1	sugars					1389:1394	the whole-cell sugars	1374:1394	the whole-cell sugars detected	1374:1403	Rhamnose, fucose and galactose were the whole-cell sugars detected.
29458489	12	2	theme	type	1769:1772	arg1	strain					1774:1779	the type strain	1765:1779	the type strain	1765:1779	Based on the results of the phenotypic and genotypic characterizations, it was concluded that the new strain represents a novel species of the genus Microbacterium, for which the name Microbacteriumtelephonicum is proposed, with S2T63T (=MCC 2967T=KACC 18715T=LMG 29293T) as the type strain.
29458489	4	3	theme	kitamiense	699:708	arg1	20510T					688:693	M. aurantiacum KACC 20510T	668:693	M. aurantiacum KACC 20510T	668:693	The DNA-DNA relatedness values of the strain S2T63T to M. aurantiacum KACC 20510T, M. kitamiense KACC 20514Tand Microbacterium laevaniformans KACC 14463T were 65 % (±4), 29.5 % (±3) and 55.9 % (±4), respectively.
29458489	4	3	theme	kitamiense	699:708	arg1	14463T					760:765	M. kitamiense KACC 20514Tand Microbacterium laevaniformans KACC 14463T	696:765	M. kitamiense KACC 20514Tand Microbacterium laevaniformans KACC 14463T	696:765	The DNA-DNA relatedness values of the strain S2T63T to M. aurantiacum KACC 20510T, M. kitamiense KACC 20514Tand Microbacterium laevaniformans KACC 14463T were 65 % (±4), 29.5 % (±3) and 55.9 % (±4), respectively.
29458489	6	4	dep	anteiso-C15 	896:907	arg1	 0					909:910	 0	909:910	 0	909:910	The major fatty acids were anteiso-C15 : 0, iso-C16 : 0, C16 : 0 and anteiso-C17 : 0.
29458489	12	5	theme	phenotypic	1518:1527	arg1	characterizations					1543:1559	the phenotypic and genotypic characterizations	1514:1559	the phenotypic and genotypic characterizations	1514:1559	Based on the results of the phenotypic and genotypic characterizations, it was concluded that the new strain represents a novel species of the genus Microbacterium, for which the name Microbacteriumtelephonicum is proposed, with S2T63T (=MCC 2967T=KACC 18715T=LMG 29293T) as the type strain.
29458489	11	6	theme	violet	1476:1481	arg1	assay					1483:1487	crystal violet assay	1468:1487	crystal violet assay	1468:1487	The strain also showed biofilm production, estimated by using crystal violet assay.
29458489	2	7	theme	type	352:355	arg1	strains					357:363	the type strains	348:363	the type strains of closely related species	348:390	A cultivation-based study of the microbial diversity of cellular phone screens led to the isolation of a Gram-stain-positive, aerobic, rod-shaped and non-endospore-forming bacterium, designated S2T63T, exhibiting phenotypic and genotypic characteristics unique to the type strains of closely related species.
29458489	2	8	theme	cultivation-based	86:102	arg1	study					104:108	A cultivation-based study	84:108	A cultivation-based study of the microbial diversity of cellular phone screens	84:161	A cultivation-based study of the microbial diversity of cellular phone screens led to the isolation of a Gram-stain-positive, aerobic, rod-shaped and non-endospore-forming bacterium, designated S2T63T, exhibiting phenotypic and genotypic characteristics unique to the type strains of closely related species.
29458489	8	9	theme	amino	1120:1124	arg1	glycine					1132:1138	glycine	1132:1138	glycine	1132:1138	The peptidoglycan contained the amino acids glycine, lysine, alanine and glutamic acid, with substantial amounts of hydroxy glutamic acid detected, which is characteristic of peptidoglycan type B1α.
29458489	8	9	theme	amino	1120:1124	arg1	alanine					1149:1155	alanine	1149:1155	alanine	1149:1155	The peptidoglycan contained the amino acids glycine, lysine, alanine and glutamic acid, with substantial amounts of hydroxy glutamic acid detected, which is characteristic of peptidoglycan type B1α.
29458489	8	9	theme	amino	1120:1124	arg1	acids					1126:1130	the amino acids	1116:1130	the amino acids glycine, lysine, alanine and glutamic acid	1116:1173	The peptidoglycan contained the amino acids glycine, lysine, alanine and glutamic acid, with substantial amounts of hydroxy glutamic acid detected, which is characteristic of peptidoglycan type B1α.
29458489	8	9	theme	amino	1120:1124	arg1	lysine					1141:1146	lysine	1141:1146	lysine	1141:1146	The peptidoglycan contained the amino acids glycine, lysine, alanine and glutamic acid, with substantial amounts of hydroxy glutamic acid detected, which is characteristic of peptidoglycan type B1α.
29458489	8	9	theme	amino	1120:1124	arg1	acid					1170:1173	glutamic acid	1161:1173	glutamic acid	1161:1173	The peptidoglycan contained the amino acids glycine, lysine, alanine and glutamic acid, with substantial amounts of hydroxy glutamic acid detected, which is characteristic of peptidoglycan type B1α.
29458489	8	10	contain	contained	1106:1114	arg2	alanine					1149:1155	alanine	1149:1155	alanine	1149:1155	The peptidoglycan contained the amino acids glycine, lysine, alanine and glutamic acid, with substantial amounts of hydroxy glutamic acid detected, which is characteristic of peptidoglycan type B1α.
29458489	8	10	contain	contained	1106:1114	arg1	peptidoglycan					1092:1104	The peptidoglycan	1088:1104	The peptidoglycan	1088:1104	The peptidoglycan contained the amino acids glycine, lysine, alanine and glutamic acid, with substantial amounts of hydroxy glutamic acid detected, which is characteristic of peptidoglycan type B1α.
29458489	8	10	contain	contained	1106:1114	arg2	acids					1126:1130	the amino acids	1116:1130	the amino acids glycine, lysine, alanine and glutamic acid	1116:1173	The peptidoglycan contained the amino acids glycine, lysine, alanine and glutamic acid, with substantial amounts of hydroxy glutamic acid detected, which is characteristic of peptidoglycan type B1α.
29458489	8	10	contain	contained	1106:1114	arg2	acid					1170:1173	glutamic acid	1161:1173	glutamic acid	1161:1173	The peptidoglycan contained the amino acids glycine, lysine, alanine and glutamic acid, with substantial amounts of hydroxy glutamic acid detected, which is characteristic of peptidoglycan type B1α.
29458489	8	10	contain	contained	1106:1114	arg2	glycine					1132:1138	glycine	1132:1138	glycine	1132:1138	The peptidoglycan contained the amino acids glycine, lysine, alanine and glutamic acid, with substantial amounts of hydroxy glutamic acid detected, which is characteristic of peptidoglycan type B1α.
29458489	8	10	contain	contained	1106:1114	arg2	lysine					1141:1146	lysine	1141:1146	lysine	1141:1146	The peptidoglycan contained the amino acids glycine, lysine, alanine and glutamic acid, with substantial amounts of hydroxy glutamic acid detected, which is characteristic of peptidoglycan type B1α.
29458489	2	11	theme	genotypic	312:320	arg1	characteristics					322:336	phenotypic and genotypic characteristics	297:336	phenotypic and genotypic characteristics unique to the type strains of closely related species	297:390	A cultivation-based study of the microbial diversity of cellular phone screens led to the isolation of a Gram-stain-positive, aerobic, rod-shaped and non-endospore-forming bacterium, designated S2T63T, exhibiting phenotypic and genotypic characteristics unique to the type strains of closely related species.
29458489	2	12	theme	cellular	140:147	arg1	screens					155:161	cellular phone screens	140:161	cellular phone screens	140:161	A cultivation-based study of the microbial diversity of cellular phone screens led to the isolation of a Gram-stain-positive, aerobic, rod-shaped and non-endospore-forming bacterium, designated S2T63T, exhibiting phenotypic and genotypic characteristics unique to the type strains of closely related species.
29458489	8	13	theme	peptidoglycan	1263:1275	arg1	B1α					1282:1284	peptidoglycan type B1α	1263:1284	peptidoglycan type B1α	1263:1284	The peptidoglycan contained the amino acids glycine, lysine, alanine and glutamic acid, with substantial amounts of hydroxy glutamic acid detected, which is characteristic of peptidoglycan type B1α.
29458489	12	14	theme	new	1588:1590	arg1	strain					1592:1597	the new strain	1584:1597	the new strain	1584:1597	Based on the results of the phenotypic and genotypic characterizations, it was concluded that the new strain represents a novel species of the genus Microbacterium, for which the name Microbacteriumtelephonicum is proposed, with S2T63T (=MCC 2967T=KACC 18715T=LMG 29293T) as the type strain.
29458489	4	15	theme	M.	696:697	arg1	20510T					688:693	M. aurantiacum KACC 20510T	668:693	M. aurantiacum KACC 20510T	668:693	The DNA-DNA relatedness values of the strain S2T63T to M. aurantiacum KACC 20510T, M. kitamiense KACC 20514Tand Microbacterium laevaniformans KACC 14463T were 65 % (±4), 29.5 % (±3) and 55.9 % (±4), respectively.
29458489	4	15	theme	M.	696:697	arg1	14463T					760:765	M. kitamiense KACC 20514Tand Microbacterium laevaniformans KACC 14463T	696:765	M. kitamiense KACC 20514Tand Microbacterium laevaniformans KACC 14463T	696:765	The DNA-DNA relatedness values of the strain S2T63T to M. aurantiacum KACC 20510T, M. kitamiense KACC 20514Tand Microbacterium laevaniformans KACC 14463T were 65 % (±4), 29.5 % (±3) and 55.9 % (±4), respectively.
29458489	5	16	theme	71.8 mol	858:865	arg1	G+C content					842:852	The genomic DNA G+C content	826:852	The genomic DNA G+C content	826:852	The genomic DNA G+C content was 71.8 mol%.
29458489	5	16	theme	71.8 mol	858:865	arg1	%					866:866	71.8 mol%	858:866	71.8 mol%	858:866	The genomic DNA G+C content was 71.8 mol%.
29458489	8	17	theme	glutamic	1212:1219	arg1	acid					1221:1224	hydroxy glutamic acid	1204:1224	hydroxy glutamic acid	1204:1224	The peptidoglycan contained the amino acids glycine, lysine, alanine and glutamic acid, with substantial amounts of hydroxy glutamic acid detected, which is characteristic of peptidoglycan type B1α.
29458489	12	18	theme	characterizations	1543:1559	arg1	results					1503:1509	the results	1499:1509	the results of the phenotypic and genotypic characterizations	1499:1559	Based on the results of the phenotypic and genotypic characterizations, it was concluded that the new strain represents a novel species of the genus Microbacterium, for which the name Microbacteriumtelephonicum is proposed, with S2T63T (=MCC 2967T=KACC 18715T=LMG 29293T) as the type strain.
29458489	3	19	theme	rRNA	428:431	arg1	sequences					438:446	16S rRNA gene sequences	424:446	16S rRNA gene sequences	424:446	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain is a member of Microbacterium, and most closely related to Microbacterium aurantiacum IFO 15234T and Microbacterium kitamiense Kitami C2T.
29458489	4	20	theme	M.	668:669	arg1	20510T					688:693	M. aurantiacum KACC 20510T	668:693	M. aurantiacum KACC 20510T	668:693	The DNA-DNA relatedness values of the strain S2T63T to M. aurantiacum KACC 20510T, M. kitamiense KACC 20514Tand Microbacterium laevaniformans KACC 14463T were 65 % (±4), 29.5 % (±3) and 55.9 % (±4), respectively.
29458489	4	20	theme	M.	668:669	arg1	14463T					760:765	M. kitamiense KACC 20514Tand Microbacterium laevaniformans KACC 14463T	696:765	M. kitamiense KACC 20514Tand Microbacterium laevaniformans KACC 14463T	696:765	The DNA-DNA relatedness values of the strain S2T63T to M. aurantiacum KACC 20510T, M. kitamiense KACC 20514Tand Microbacterium laevaniformans KACC 14463T were 65 % (±4), 29.5 % (±3) and 55.9 % (±4), respectively.
29458489	2	21	theme	diversity	127:135	arg1	study					104:108	A cultivation-based study	84:108	A cultivation-based study of the microbial diversity of cellular phone screens	84:161	A cultivation-based study of the microbial diversity of cellular phone screens led to the isolation of a Gram-stain-positive, aerobic, rod-shaped and non-endospore-forming bacterium, designated S2T63T, exhibiting phenotypic and genotypic characteristics unique to the type strains of closely related species.
29458489	8	22	theme	acid	1221:1224	arg1	amounts					1193:1199	substantial amounts	1181:1199	substantial amounts of hydroxy glutamic acid detected, which is characteristic of peptidoglycan type B1α	1181:1284	The peptidoglycan contained the amino acids glycine, lysine, alanine and glutamic acid, with substantial amounts of hydroxy glutamic acid detected, which is characteristic of peptidoglycan type B1α.
29458489	8	22	theme	acid	1221:1224	arg1	acid					1221:1224	hydroxy glutamic acid	1204:1224	hydroxy glutamic acid	1204:1224	The peptidoglycan contained the amino acids glycine, lysine, alanine and glutamic acid, with substantial amounts of hydroxy glutamic acid detected, which is characteristic of peptidoglycan type B1α.
29458489	3	23	theme	Phylogenetic	393:404	arg1	analysis					406:413	Phylogenetic analysis	393:413	Phylogenetic analysis based on 16S rRNA gene sequences	393:446	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain is a member of Microbacterium, and most closely related to Microbacterium aurantiacum IFO 15234T and Microbacterium kitamiense Kitami C2T.
29458489	8	24	theme	glutamic	1161:1168	arg1	acid					1170:1173	glutamic acid	1161:1173	glutamic acid	1161:1173	The peptidoglycan contained the amino acids glycine, lysine, alanine and glutamic acid, with substantial amounts of hydroxy glutamic acid detected, which is characteristic of peptidoglycan type B1α.
29458489	8	24	theme	glutamic	1161:1168	arg1	acids					1126:1130	the amino acids	1116:1130	the amino acids glycine, lysine, alanine and glutamic acid	1116:1173	The peptidoglycan contained the amino acids glycine, lysine, alanine and glutamic acid, with substantial amounts of hydroxy glutamic acid detected, which is characteristic of peptidoglycan type B1α.
29458489	0	25	theme	telephonicum	15:26	arg1	sp					28:29	Microbacterium telephonicum sp	0:29	Microbacterium telephonicum sp.	0:30	Microbacterium telephonicum sp.
29458489	2	26	theme	microbial	117:125	arg1	diversity					127:135	the microbial diversity	113:135	the microbial diversity of cellular phone screens	113:161	A cultivation-based study of the microbial diversity of cellular phone screens led to the isolation of a Gram-stain-positive, aerobic, rod-shaped and non-endospore-forming bacterium, designated S2T63T, exhibiting phenotypic and genotypic characteristics unique to the type strains of closely related species.
29458489	1	27	theme	cellular	68:75	arg1	phone					77:81	a cellular phone	66:81	a cellular phone	66:81	nov., isolated from the screen of a cellular phone.
29458489	2	28	theme	species	384:390	arg1	strains					357:363	the type strains	348:363	the type strains of closely related species	348:390	A cultivation-based study of the microbial diversity of cellular phone screens led to the isolation of a Gram-stain-positive, aerobic, rod-shaped and non-endospore-forming bacterium, designated S2T63T, exhibiting phenotypic and genotypic characteristics unique to the type strains of closely related species.
29458489	0	29	theme	Microbacterium	0:13	arg1	sp					28:29	Microbacterium telephonicum sp	0:29	Microbacterium telephonicum sp.	0:30	Microbacterium telephonicum sp.
29458489	12	30	theme	genotypic	1533:1541	arg1	characterizations					1543:1559	the phenotypic and genotypic characterizations	1514:1559	the phenotypic and genotypic characterizations	1514:1559	Based on the results of the phenotypic and genotypic characterizations, it was concluded that the new strain represents a novel species of the genus Microbacterium, for which the name Microbacteriumtelephonicum is proposed, with S2T63T (=MCC 2967T=KACC 18715T=LMG 29293T) as the type strain.
29458489	12	31	theme	name	1669:1672	arg1	Microbacteriumtelephonicum					1674:1699	the name Microbacteriumtelephonicum	1665:1699	the name Microbacteriumtelephonicum	1665:1699	Based on the results of the phenotypic and genotypic characterizations, it was concluded that the new strain represents a novel species of the genus Microbacterium, for which the name Microbacteriumtelephonicum is proposed, with S2T63T (=MCC 2967T=KACC 18715T=LMG 29293T) as the type strain.
29458489	1	32	theme	phone	77:81	arg1	screen					56:61	the screen	52:61	the screen of a cellular phone	52:81	nov., isolated from the screen of a cellular phone.
29458489	2	33	theme	related	376:382	arg1	species					384:390	closely related species	368:390	closely related species	368:390	A cultivation-based study of the microbial diversity of cellular phone screens led to the isolation of a Gram-stain-positive, aerobic, rod-shaped and non-endospore-forming bacterium, designated S2T63T, exhibiting phenotypic and genotypic characteristics unique to the type strains of closely related species.
29458489	7	34	theme	polar	964:968	arg1	diphosphatidylglycerol					982:1003	diphosphatidylglycerol	982:1003	diphosphatidylglycerol	982:1003	The main polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and two unidentified polar lipids.
29458489	7	34	theme	polar	964:968	arg1	lipids					970:975	The main polar lipids	955:975	The main polar lipids	955:975	The main polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and two unidentified polar lipids.
29458489	4	35	theme	KACC	710:713	arg1	20510T					688:693	M. aurantiacum KACC 20510T	668:693	M. aurantiacum KACC 20510T	668:693	The DNA-DNA relatedness values of the strain S2T63T to M. aurantiacum KACC 20510T, M. kitamiense KACC 20514Tand Microbacterium laevaniformans KACC 14463T were 65 % (±4), 29.5 % (±3) and 55.9 % (±4), respectively.
29458489	4	35	theme	KACC	710:713	arg1	14463T					760:765	M. kitamiense KACC 20514Tand Microbacterium laevaniformans KACC 14463T	696:765	M. kitamiense KACC 20514Tand Microbacterium laevaniformans KACC 14463T	696:765	The DNA-DNA relatedness values of the strain S2T63T to M. aurantiacum KACC 20510T, M. kitamiense KACC 20514Tand Microbacterium laevaniformans KACC 14463T were 65 % (±4), 29.5 % (±3) and 55.9 % (±4), respectively.
29458489	2	36	theme	phenotypic	297:306	arg1	characteristics					322:336	phenotypic and genotypic characteristics	297:336	phenotypic and genotypic characteristics unique to the type strains of closely related species	297:390	A cultivation-based study of the microbial diversity of cellular phone screens led to the isolation of a Gram-stain-positive, aerobic, rod-shaped and non-endospore-forming bacterium, designated S2T63T, exhibiting phenotypic and genotypic characteristics unique to the type strains of closely related species.
29458489	4	37	theme	KACC	755:758	arg1	20510T					688:693	M. aurantiacum KACC 20510T	668:693	M. aurantiacum KACC 20510T	668:693	The DNA-DNA relatedness values of the strain S2T63T to M. aurantiacum KACC 20510T, M. kitamiense KACC 20514Tand Microbacterium laevaniformans KACC 14463T were 65 % (±4), 29.5 % (±3) and 55.9 % (±4), respectively.
29458489	4	37	theme	KACC	755:758	arg1	14463T					760:765	M. kitamiense KACC 20514Tand Microbacterium laevaniformans KACC 14463T	696:765	M. kitamiense KACC 20514Tand Microbacterium laevaniformans KACC 14463T	696:765	The DNA-DNA relatedness values of the strain S2T63T to M. aurantiacum KACC 20510T, M. kitamiense KACC 20514Tand Microbacterium laevaniformans KACC 14463T were 65 % (±4), 29.5 % (±3) and 55.9 % (±4), respectively.
29458489	3	38	theme	15234T	564:569	arg1	strain					467:472	the strain	463:472	the strain	463:472	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain is a member of Microbacterium, and most closely related to Microbacterium aurantiacum IFO 15234T and Microbacterium kitamiense Kitami C2T.
29458489	3	38	theme	15234T	564:569	arg1	member					479:484	a member	477:484	a member of Microbacterium, and most closely related to Microbacterium aurantiacum IFO 15234T and Microbacterium kitamiense Kitami C2T	477:610	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain is a member of Microbacterium, and most closely related to Microbacterium aurantiacum IFO 15234T and Microbacterium kitamiense Kitami C2T.
29458489	8	39	theme	substantial	1181:1191	arg1	amounts					1193:1199	substantial amounts	1181:1199	substantial amounts of hydroxy glutamic acid detected, which is characteristic of peptidoglycan type B1α	1181:1284	The peptidoglycan contained the amino acids glycine, lysine, alanine and glutamic acid, with substantial amounts of hydroxy glutamic acid detected, which is characteristic of peptidoglycan type B1α.
29458489	8	39	theme	substantial	1181:1191	arg1	acid					1221:1224	hydroxy glutamic acid	1204:1224	hydroxy glutamic acid	1204:1224	The peptidoglycan contained the amino acids glycine, lysine, alanine and glutamic acid, with substantial amounts of hydroxy glutamic acid detected, which is characteristic of peptidoglycan type B1α.
29458489	4	40	theme	KACC	683:686	arg1	20510T					688:693	M. aurantiacum KACC 20510T	668:693	M. aurantiacum KACC 20510T	668:693	The DNA-DNA relatedness values of the strain S2T63T to M. aurantiacum KACC 20510T, M. kitamiense KACC 20514Tand Microbacterium laevaniformans KACC 14463T were 65 % (±4), 29.5 % (±3) and 55.9 % (±4), respectively.
29458489	4	40	theme	KACC	683:686	arg1	14463T					760:765	M. kitamiense KACC 20514Tand Microbacterium laevaniformans KACC 14463T	696:765	M. kitamiense KACC 20514Tand Microbacterium laevaniformans KACC 14463T	696:765	The DNA-DNA relatedness values of the strain S2T63T to M. aurantiacum KACC 20510T, M. kitamiense KACC 20514Tand Microbacterium laevaniformans KACC 14463T were 65 % (±4), 29.5 % (±3) and 55.9 % (±4), respectively.
29458489	8	41	theme	hydroxy	1204:1210	arg1	acid					1221:1224	hydroxy glutamic acid	1204:1224	hydroxy glutamic acid	1204:1224	The peptidoglycan contained the amino acids glycine, lysine, alanine and glutamic acid, with substantial amounts of hydroxy glutamic acid detected, which is characteristic of peptidoglycan type B1α.
29458489	3	42	theme	gene	433:436	arg1	sequences					438:446	16S rRNA gene sequences	424:446	16S rRNA gene sequences	424:446	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain is a member of Microbacterium, and most closely related to Microbacterium aurantiacum IFO 15234T and Microbacterium kitamiense Kitami C2T.
29458489	4	43	theme	S2T63T	658:663	arg1	%					775:775	65 %	772:775	65 % (±4)	772:780	The DNA-DNA relatedness values of the strain S2T63T to M. aurantiacum KACC 20510T, M. kitamiense KACC 20514Tand Microbacterium laevaniformans KACC 14463T were 65 % (±4), 29.5 % (±3) and 55.9 % (±4), respectively.
29458489	4	43	theme	S2T63T	658:663	arg1	values					637:642	The DNA-DNA relatedness values	613:642	The DNA-DNA relatedness values of the strain S2T63T to M. aurantiacum KACC 20510T, M. kitamiense KACC 20514Tand Microbacterium laevaniformans KACC 14463T	613:765	The DNA-DNA relatedness values of the strain S2T63T to M. aurantiacum KACC 20510T, M. kitamiense KACC 20514Tand Microbacterium laevaniformans KACC 14463T were 65 % (±4), 29.5 % (±3) and 55.9 % (±4), respectively.
29458489	3	44	theme	16S	424:426	arg1	sequences					438:446	16S rRNA gene sequences	424:446	16S rRNA gene sequences	424:446	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain is a member of Microbacterium, and most closely related to Microbacterium aurantiacum IFO 15234T and Microbacterium kitamiense Kitami C2T.
29458489	7	45	theme	main	959:962	arg1	diphosphatidylglycerol					982:1003	diphosphatidylglycerol	982:1003	diphosphatidylglycerol	982:1003	The main polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and two unidentified polar lipids.
29458489	7	45	theme	main	959:962	arg1	lipids					970:975	The main polar lipids	955:975	The main polar lipids	955:975	The main polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and two unidentified polar lipids.
29458489	2	46	theme	screens	155:161	arg1	diversity					127:135	the microbial diversity	113:135	the microbial diversity of cellular phone screens	113:161	A cultivation-based study of the microbial diversity of cellular phone screens led to the isolation of a Gram-stain-positive, aerobic, rod-shaped and non-endospore-forming bacterium, designated S2T63T, exhibiting phenotypic and genotypic characteristics unique to the type strains of closely related species.
29458489	2	47	theme	aerobic	210:216	arg1	bacterium					256:264	a Gram-stain-positive, aerobic, rod-shaped and non-endospore-forming bacterium	187:264	a Gram-stain-positive, aerobic, rod-shaped and non-endospore-forming bacterium	187:264	A cultivation-based study of the microbial diversity of cellular phone screens led to the isolation of a Gram-stain-positive, aerobic, rod-shaped and non-endospore-forming bacterium, designated S2T63T, exhibiting phenotypic and genotypic characteristics unique to the type strains of closely related species.
29458489	12	48	theme	18715T=LMG	1743:1752	arg1	29293T					1754:1759	=MCC 2967T=KACC 18715T=LMG 29293T	1727:1759	=MCC 2967T=KACC 18715T=LMG 29293T	1727:1759	Based on the results of the phenotypic and genotypic characterizations, it was concluded that the new strain represents a novel species of the genus Microbacterium, for which the name Microbacteriumtelephonicum is proposed, with S2T63T (=MCC 2967T=KACC 18715T=LMG 29293T) as the type strain.
29458489	12	48	theme	18715T=LMG	1743:1752	arg1	S2T63T					1719:1724	S2T63T	1719:1724	S2T63T (=MCC 2967T=KACC 18715T=LMG 29293T) as the type strain	1719:1779	Based on the results of the phenotypic and genotypic characterizations, it was concluded that the new strain represents a novel species of the genus Microbacterium, for which the name Microbacteriumtelephonicum is proposed, with S2T63T (=MCC 2967T=KACC 18715T=LMG 29293T) as the type strain.
29458489	4	49	theme	strain	651:656	arg1	S2T63T					658:663	the strain S2T63T	647:663	the strain S2T63T to M. aurantiacum KACC 20510T, M. kitamiense KACC 20514Tand Microbacterium laevaniformans KACC 14463T	647:765	The DNA-DNA relatedness values of the strain S2T63T to M. aurantiacum KACC 20510T, M. kitamiense KACC 20514Tand Microbacterium laevaniformans KACC 14463T were 65 % (±4), 29.5 % (±3) and 55.9 % (±4), respectively.
29458489	7	50	theme	unidentified	1061:1072	arg1	lipids					1080:1085	two unidentified polar lipids	1057:1085	two unidentified polar lipids	1057:1085	The main polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and two unidentified polar lipids.
29458489	9	51	theme	predominant	1291:1301	arg1	MK-12					1321:1325	MK-12	1321:1325	MK-12	1321:1325	The predominant menaquinones were MK-12 and MK-13.
29458489	9	51	theme	predominant	1291:1301	arg1	menaquinones					1303:1314	The predominant menaquinones	1287:1314	The predominant menaquinones	1287:1314	The predominant menaquinones were MK-12 and MK-13.
29458489	8	52	theme	type	1277:1280	arg1	B1α					1282:1284	peptidoglycan type B1α	1263:1284	peptidoglycan type B1α	1263:1284	The peptidoglycan contained the amino acids glycine, lysine, alanine and glutamic acid, with substantial amounts of hydroxy glutamic acid detected, which is characteristic of peptidoglycan type B1α.
29458489	2	53	theme	phone	149:153	arg1	screens					155:161	cellular phone screens	140:161	cellular phone screens	140:161	A cultivation-based study of the microbial diversity of cellular phone screens led to the isolation of a Gram-stain-positive, aerobic, rod-shaped and non-endospore-forming bacterium, designated S2T63T, exhibiting phenotypic and genotypic characteristics unique to the type strains of closely related species.
29458489	12	54	theme	2967T=KACC	1732:1741	arg1	29293T					1754:1759	=MCC 2967T=KACC 18715T=LMG 29293T	1727:1759	=MCC 2967T=KACC 18715T=LMG 29293T	1727:1759	Based on the results of the phenotypic and genotypic characterizations, it was concluded that the new strain represents a novel species of the genus Microbacterium, for which the name Microbacteriumtelephonicum is proposed, with S2T63T (=MCC 2967T=KACC 18715T=LMG 29293T) as the type strain.
29458489	12	54	theme	2967T=KACC	1732:1741	arg1	S2T63T					1719:1724	S2T63T	1719:1724	S2T63T (=MCC 2967T=KACC 18715T=LMG 29293T) as the type strain	1719:1779	Based on the results of the phenotypic and genotypic characterizations, it was concluded that the new strain represents a novel species of the genus Microbacterium, for which the name Microbacteriumtelephonicum is proposed, with S2T63T (=MCC 2967T=KACC 18715T=LMG 29293T) as the type strain.
29458489	4	55	theme	20514Tand	715:723	arg1	20510T					688:693	M. aurantiacum KACC 20510T	668:693	M. aurantiacum KACC 20510T	668:693	The DNA-DNA relatedness values of the strain S2T63T to M. aurantiacum KACC 20510T, M. kitamiense KACC 20514Tand Microbacterium laevaniformans KACC 14463T were 65 % (±4), 29.5 % (±3) and 55.9 % (±4), respectively.
29458489	4	55	theme	20514Tand	715:723	arg1	14463T					760:765	M. kitamiense KACC 20514Tand Microbacterium laevaniformans KACC 14463T	696:765	M. kitamiense KACC 20514Tand Microbacterium laevaniformans KACC 14463T	696:765	The DNA-DNA relatedness values of the strain S2T63T to M. aurantiacum KACC 20510T, M. kitamiense KACC 20514Tand Microbacterium laevaniformans KACC 14463T were 65 % (±4), 29.5 % (±3) and 55.9 % (±4), respectively.
29458489	7	56	theme	polar	1074:1078	arg1	lipids					1080:1085	two unidentified polar lipids	1057:1085	two unidentified polar lipids	1057:1085	The main polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and two unidentified polar lipids.
29458489	6	57	theme	fatty	879:883	arg1	acids					885:889	The major fatty acids	869:889	The major fatty acids	869:889	The major fatty acids were anteiso-C15 : 0, iso-C16 : 0, C16 : 0 and anteiso-C17 : 0.
29458489	6	57	theme	fatty	879:883	arg1	anteiso-C15 					896:907	anteiso-C15 	896:907	anteiso-C15 	896:907	The major fatty acids were anteiso-C15 : 0, iso-C16 : 0, C16 : 0 and anteiso-C17 : 0.
29458489	2	58	theme	Gram-stain-positive	189:207	arg1	bacterium					256:264	a Gram-stain-positive, aerobic, rod-shaped and non-endospore-forming bacterium	187:264	a Gram-stain-positive, aerobic, rod-shaped and non-endospore-forming bacterium	187:264	A cultivation-based study of the microbial diversity of cellular phone screens led to the isolation of a Gram-stain-positive, aerobic, rod-shaped and non-endospore-forming bacterium, designated S2T63T, exhibiting phenotypic and genotypic characteristics unique to the type strains of closely related species.
29458489	3	59	theme	Microbacterium	489:502	arg1	strain					467:472	the strain	463:472	the strain	463:472	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain is a member of Microbacterium, and most closely related to Microbacterium aurantiacum IFO 15234T and Microbacterium kitamiense Kitami C2T.
29458489	3	59	theme	Microbacterium	489:502	arg1	member					479:484	a member	477:484	a member of Microbacterium, and most closely related to Microbacterium aurantiacum IFO 15234T and Microbacterium kitamiense Kitami C2T	477:610	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain is a member of Microbacterium, and most closely related to Microbacterium aurantiacum IFO 15234T and Microbacterium kitamiense Kitami C2T.
29458489	12	60	theme	genus	1633:1637	arg1	Microbacterium					1639:1652	the genus Microbacterium	1629:1652	the genus Microbacterium	1629:1652	Based on the results of the phenotypic and genotypic characterizations, it was concluded that the new strain represents a novel species of the genus Microbacterium, for which the name Microbacteriumtelephonicum is proposed, with S2T63T (=MCC 2967T=KACC 18715T=LMG 29293T) as the type strain.
29458489	6	61	theme	major	873:877	arg1	acids					885:889	The major fatty acids	869:889	The major fatty acids	869:889	The major fatty acids were anteiso-C15 : 0, iso-C16 : 0, C16 : 0 and anteiso-C17 : 0.
29458489	6	61	theme	major	873:877	arg1	anteiso-C15 					896:907	anteiso-C15 	896:907	anteiso-C15 	896:907	The major fatty acids were anteiso-C15 : 0, iso-C16 : 0, C16 : 0 and anteiso-C17 : 0.
29458489	8	62	dep	acids	1126:1130	arg1	glycine					1132:1138	glycine	1132:1138	glycine	1132:1138	The peptidoglycan contained the amino acids glycine, lysine, alanine and glutamic acid, with substantial amounts of hydroxy glutamic acid detected, which is characteristic of peptidoglycan type B1α.
29458489	8	62	dep	acids	1126:1130	arg1	alanine					1149:1155	alanine	1149:1155	alanine	1149:1155	The peptidoglycan contained the amino acids glycine, lysine, alanine and glutamic acid, with substantial amounts of hydroxy glutamic acid detected, which is characteristic of peptidoglycan type B1α.
29458489	8	62	dep	acids	1126:1130	arg1	acid					1170:1173	glutamic acid	1161:1173	glutamic acid	1161:1173	The peptidoglycan contained the amino acids glycine, lysine, alanine and glutamic acid, with substantial amounts of hydroxy glutamic acid detected, which is characteristic of peptidoglycan type B1α.
29458489	8	62	dep	acids	1126:1130	arg1	lysine					1141:1146	lysine	1141:1146	lysine	1141:1146	The peptidoglycan contained the amino acids glycine, lysine, alanine and glutamic acid, with substantial amounts of hydroxy glutamic acid detected, which is characteristic of peptidoglycan type B1α.
29458489	8	62	dep	acids	1126:1130	arg1	acids					1126:1130	the amino acids	1116:1130	the amino acids glycine, lysine, alanine and glutamic acid	1116:1173	The peptidoglycan contained the amino acids glycine, lysine, alanine and glutamic acid, with substantial amounts of hydroxy glutamic acid detected, which is characteristic of peptidoglycan type B1α.
29458489	4	63	theme	aurantiacum	671:681	arg1	20510T					688:693	M. aurantiacum KACC 20510T	668:693	M. aurantiacum KACC 20510T	668:693	The DNA-DNA relatedness values of the strain S2T63T to M. aurantiacum KACC 20510T, M. kitamiense KACC 20514Tand Microbacterium laevaniformans KACC 14463T were 65 % (±4), 29.5 % (±3) and 55.9 % (±4), respectively.
29458489	4	63	theme	aurantiacum	671:681	arg1	14463T					760:765	M. kitamiense KACC 20514Tand Microbacterium laevaniformans KACC 14463T	696:765	M. kitamiense KACC 20514Tand Microbacterium laevaniformans KACC 14463T	696:765	The DNA-DNA relatedness values of the strain S2T63T to M. aurantiacum KACC 20510T, M. kitamiense KACC 20514Tand Microbacterium laevaniformans KACC 14463T were 65 % (±4), 29.5 % (±3) and 55.9 % (±4), respectively.
29458489	2	64	theme	bacterium	256:264	arg1	isolation					174:182	the isolation	170:182	the isolation	170:182	A cultivation-based study of the microbial diversity of cellular phone screens led to the isolation of a Gram-stain-positive, aerobic, rod-shaped and non-endospore-forming bacterium, designated S2T63T, exhibiting phenotypic and genotypic characteristics unique to the type strains of closely related species.
29458489	2	65	theme	unique	338:343	arg1	characteristics					322:336	phenotypic and genotypic characteristics	297:336	phenotypic and genotypic characteristics unique to the type strains of closely related species	297:390	A cultivation-based study of the microbial diversity of cellular phone screens led to the isolation of a Gram-stain-positive, aerobic, rod-shaped and non-endospore-forming bacterium, designated S2T63T, exhibiting phenotypic and genotypic characteristics unique to the type strains of closely related species.
29458489	11	66	theme	biofilm	1429:1435	arg1	production					1437:1446	biofilm production	1429:1446	biofilm production	1429:1446	The strain also showed biofilm production, estimated by using crystal violet assay.
29458489	4	67	theme	DNA-DNA	617:623	arg1	%					775:775	65 %	772:775	65 % (±4)	772:780	The DNA-DNA relatedness values of the strain S2T63T to M. aurantiacum KACC 20510T, M. kitamiense KACC 20514Tand Microbacterium laevaniformans KACC 14463T were 65 % (±4), 29.5 % (±3) and 55.9 % (±4), respectively.
29458489	4	67	theme	DNA-DNA	617:623	arg1	values					637:642	The DNA-DNA relatedness values	613:642	The DNA-DNA relatedness values of the strain S2T63T to M. aurantiacum KACC 20510T, M. kitamiense KACC 20514Tand Microbacterium laevaniformans KACC 14463T	613:765	The DNA-DNA relatedness values of the strain S2T63T to M. aurantiacum KACC 20510T, M. kitamiense KACC 20514Tand Microbacterium laevaniformans KACC 14463T were 65 % (±4), 29.5 % (±3) and 55.9 % (±4), respectively.
29458489	8	68	theme	B1α	1282:1284	arg1	characteristic					1245:1258	characteristic	1245:1258	characteristic of peptidoglycan type B1α	1245:1284	The peptidoglycan contained the amino acids glycine, lysine, alanine and glutamic acid, with substantial amounts of hydroxy glutamic acid detected, which is characteristic of peptidoglycan type B1α.
29458489	2	69	theme	non-endospore-forming	234:254	arg1	bacterium					256:264	a Gram-stain-positive, aerobic, rod-shaped and non-endospore-forming bacterium	187:264	a Gram-stain-positive, aerobic, rod-shaped and non-endospore-forming bacterium	187:264	A cultivation-based study of the microbial diversity of cellular phone screens led to the isolation of a Gram-stain-positive, aerobic, rod-shaped and non-endospore-forming bacterium, designated S2T63T, exhibiting phenotypic and genotypic characteristics unique to the type strains of closely related species.
29458489	4	70	theme	Microbacterium	725:738	arg1	20510T					688:693	M. aurantiacum KACC 20510T	668:693	M. aurantiacum KACC 20510T	668:693	The DNA-DNA relatedness values of the strain S2T63T to M. aurantiacum KACC 20510T, M. kitamiense KACC 20514Tand Microbacterium laevaniformans KACC 14463T were 65 % (±4), 29.5 % (±3) and 55.9 % (±4), respectively.
29458489	4	70	theme	Microbacterium	725:738	arg1	14463T					760:765	M. kitamiense KACC 20514Tand Microbacterium laevaniformans KACC 14463T	696:765	M. kitamiense KACC 20514Tand Microbacterium laevaniformans KACC 14463T	696:765	The DNA-DNA relatedness values of the strain S2T63T to M. aurantiacum KACC 20510T, M. kitamiense KACC 20514Tand Microbacterium laevaniformans KACC 14463T were 65 % (±4), 29.5 % (±3) and 55.9 % (±4), respectively.
29458489	4	71	theme	laevaniformans	740:753	arg1	20510T					688:693	M. aurantiacum KACC 20510T	668:693	M. aurantiacum KACC 20510T	668:693	The DNA-DNA relatedness values of the strain S2T63T to M. aurantiacum KACC 20510T, M. kitamiense KACC 20514Tand Microbacterium laevaniformans KACC 14463T were 65 % (±4), 29.5 % (±3) and 55.9 % (±4), respectively.
29458489	4	71	theme	laevaniformans	740:753	arg1	14463T					760:765	M. kitamiense KACC 20514Tand Microbacterium laevaniformans KACC 14463T	696:765	M. kitamiense KACC 20514Tand Microbacterium laevaniformans KACC 14463T	696:765	The DNA-DNA relatedness values of the strain S2T63T to M. aurantiacum KACC 20510T, M. kitamiense KACC 20514Tand Microbacterium laevaniformans KACC 14463T were 65 % (±4), 29.5 % (±3) and 55.9 % (±4), respectively.
29458489	6	72	dep	 0	909:910	arg1	 0					951:952	 0	951:952	 0	951:952	The major fatty acids were anteiso-C15 : 0, iso-C16 : 0, C16 : 0 and anteiso-C17 : 0.
29458489	6	72	dep	 0	909:910	arg1	anteiso-C17 					938:949	anteiso-C17 	938:949	anteiso-C17 	938:949	The major fatty acids were anteiso-C15 : 0, iso-C16 : 0, C16 : 0 and anteiso-C17 : 0.
29458489	6	72	dep	 0	909:910	arg1	 0					931:932	 0	931:932	 0	931:932	The major fatty acids were anteiso-C15 : 0, iso-C16 : 0, C16 : 0 and anteiso-C17 : 0.
29458489	6	72	dep	 0	909:910	arg1	C16 					926:929	C16 	926:929	C16 	926:929	The major fatty acids were anteiso-C15 : 0, iso-C16 : 0, C16 : 0 and anteiso-C17 : 0.
29458489	5	73	theme	genomic	830:836	arg1	G+C content					842:852	The genomic DNA G+C content	826:852	The genomic DNA G+C content	826:852	The genomic DNA G+C content was 71.8 mol%.
29458489	5	73	theme	genomic	830:836	arg1	%					866:866	71.8 mol%	858:866	71.8 mol%	858:866	The genomic DNA G+C content was 71.8 mol%.
29458489	12	74	theme	Microbacterium	1639:1652	arg1	species					1618:1624	a novel species	1610:1624	a novel species	1610:1624	Based on the results of the phenotypic and genotypic characterizations, it was concluded that the new strain represents a novel species of the genus Microbacterium, for which the name Microbacteriumtelephonicum is proposed, with S2T63T (=MCC 2967T=KACC 18715T=LMG 29293T) as the type strain.
29458489	2	75	theme	rod-shaped	219:228	arg1	bacterium					256:264	a Gram-stain-positive, aerobic, rod-shaped and non-endospore-forming bacterium	187:264	a Gram-stain-positive, aerobic, rod-shaped and non-endospore-forming bacterium	187:264	A cultivation-based study of the microbial diversity of cellular phone screens led to the isolation of a Gram-stain-positive, aerobic, rod-shaped and non-endospore-forming bacterium, designated S2T63T, exhibiting phenotypic and genotypic characteristics unique to the type strains of closely related species.
29458489	12	76	theme	=MCC	1727:1730	arg1	29293T					1754:1759	=MCC 2967T=KACC 18715T=LMG 29293T	1727:1759	=MCC 2967T=KACC 18715T=LMG 29293T	1727:1759	Based on the results of the phenotypic and genotypic characterizations, it was concluded that the new strain represents a novel species of the genus Microbacterium, for which the name Microbacteriumtelephonicum is proposed, with S2T63T (=MCC 2967T=KACC 18715T=LMG 29293T) as the type strain.
29458489	12	76	theme	=MCC	1727:1730	arg1	S2T63T					1719:1724	S2T63T	1719:1724	S2T63T (=MCC 2967T=KACC 18715T=LMG 29293T) as the type strain	1719:1779	Based on the results of the phenotypic and genotypic characterizations, it was concluded that the new strain represents a novel species of the genus Microbacterium, for which the name Microbacteriumtelephonicum is proposed, with S2T63T (=MCC 2967T=KACC 18715T=LMG 29293T) as the type strain.
29458489	5	77	theme	DNA	838:840	arg1	G+C content					842:852	The genomic DNA G+C content	826:852	The genomic DNA G+C content	826:852	The genomic DNA G+C content was 71.8 mol%.
29458489	5	77	theme	DNA	838:840	arg1	%					866:866	71.8 mol%	858:866	71.8 mol%	858:866	The genomic DNA G+C content was 71.8 mol%.
29458489	12	78	theme	novel	1612:1616	arg1	species					1618:1624	a novel species	1610:1624	a novel species	1610:1624	Based on the results of the phenotypic and genotypic characterizations, it was concluded that the new strain represents a novel species of the genus Microbacterium, for which the name Microbacteriumtelephonicum is proposed, with S2T63T (=MCC 2967T=KACC 18715T=LMG 29293T) as the type strain.
29458489	4	79	theme	relatedness	625:635	arg1	%					775:775	65 %	772:775	65 % (±4)	772:780	The DNA-DNA relatedness values of the strain S2T63T to M. aurantiacum KACC 20510T, M. kitamiense KACC 20514Tand Microbacterium laevaniformans KACC 14463T were 65 % (±4), 29.5 % (±3) and 55.9 % (±4), respectively.
29458489	4	79	theme	relatedness	625:635	arg1	values					637:642	The DNA-DNA relatedness values	613:642	The DNA-DNA relatedness values of the strain S2T63T to M. aurantiacum KACC 20510T, M. kitamiense KACC 20514Tand Microbacterium laevaniformans KACC 14463T	613:765	The DNA-DNA relatedness values of the strain S2T63T to M. aurantiacum KACC 20510T, M. kitamiense KACC 20514Tand Microbacterium laevaniformans KACC 14463T were 65 % (±4), 29.5 % (±3) and 55.9 % (±4), respectively.
30312415	8	0	theme	Il10-/-	1115:1121	arg1	mice					1123:1126	DSS-treated or Il10-/- mice	1100:1126	DSS-treated or Il10-/- mice	1100:1126	The presence of Casp11 did not alter the severity of chronic colitis in DSS-treated or Il10-/- mice.
30312415	4	1	theme	Casp11	558:563	arg1	colitis					607:613	acute and chronic DSS colitis	585:613	acute and chronic DSS colitis	585:613	Methods We studied the role of Casp11 in Il10-/- mice and acute and chronic DSS colitis mouse models.
30312415	4	1	theme	Casp11	558:563	arg1	role					550:553	the role	546:553	the role of Casp11 in Il10-/- mice	546:579	Methods We studied the role of Casp11 in Il10-/- mice and acute and chronic DSS colitis mouse models.
30312415	11	2	theme	stochastic	1345:1354	arg1	changes					1356:1362	pronounced stochastic changes	1334:1362	pronounced stochastic changes	1334:1362	There were pronounced stochastic changes in the fecal microbiome over this time.
30312415	9	3	theme	prior	1141:1145	arg1	reports					1147:1153	prior reports	1141:1153	prior reports	1141:1153	Contrary to prior reports, we initially observed that Casp11 exacerbates acute DSS colitis.
30312415	3	4	theme	acute	496:500	arg1	colitis					506:512	acute DSS colitis	496:512	acute DSS colitis	496:512	However, the effects of Casp11 on chronic experimental colitis and factors that influence the impact of Casp11 on acute DSS colitis are unknown.
30312415	12	5	theme	bacterial	1420:1428	arg1	taxa					1430:1433	bacterial taxa	1420:1433	bacterial taxa that changed over time in wild-type vs Casp11-/- mice	1420:1487	The majority of bacterial taxa that changed over time in wild-type vs Casp11-/- mice belong to the Clostridiales.
30312415	4	6	theme	Il10-/-	568:574	arg1	mice					576:579	Il10-/- mice	568:579	Il10-/- mice	568:579	Methods We studied the role of Casp11 in Il10-/- mice and acute and chronic DSS colitis mouse models.
30312415	7	7	dep	Results	906:912	arg1	detected					917:924	detected	917:924	detected increased colonic Casp11 mRNA in Il10-/- mice with chronic colitis, but not in mice with DSS colitis	917:1025	Results We detected increased colonic Casp11 mRNA in Il10-/- mice with chronic colitis, but not in mice with DSS colitis.
30312415	6	8	theme	16S	881:883	arg1	sequencing					894:903	16S amplicon sequencing	881:903	16S amplicon sequencing	881:903	We determined fecal microbial composition using 16S amplicon sequencing.
30312415	11	9	theme	fecal	1371:1375	arg1	microbiome					1377:1386	the fecal microbiome	1367:1386	the fecal microbiome	1367:1386	There were pronounced stochastic changes in the fecal microbiome over this time.
30312415	2	10	theme	activator	278:286	arg1	Casp11					300:305	Casp11	300:305	Casp11	300:305	The noncanonical inflammasome activator caspase-11 (Casp11) reportedly attenuates acute dextran sodium sulfate (DSS) colitis in mice.
30312415	2	10	theme	activator	278:286	arg1	caspase-11					288:297	The noncanonical inflammasome activator caspase-11	248:297	The noncanonical inflammasome activator caspase-11 (Casp11)	248:306	The noncanonical inflammasome activator caspase-11 (Casp11) reportedly attenuates acute dextran sodium sulfate (DSS) colitis in mice.
30312415	1	11	theme	inflammatory	178:189	arg1	diseases					197:204	inflammatory bowel diseases	178:204	inflammatory bowel diseases	178:204	Background Human and mouse studies implicate the inflammasome in the pathogenesis of inflammatory bowel diseases, though the effects in mice are variable.
30312415	1	12	theme	diseases	197:204	arg1	pathogenesis					162:173	the pathogenesis	158:173	the pathogenesis of inflammatory bowel diseases	158:204	Background Human and mouse studies implicate the inflammasome in the pathogenesis of inflammatory bowel diseases, though the effects in mice are variable.
30312415	2	13	from	colitis	365:371	arg1	mice					376:379	mice	376:379	mice	376:379	The noncanonical inflammasome activator caspase-11 (Casp11) reportedly attenuates acute dextran sodium sulfate (DSS) colitis in mice.
30312415	4	14	theme	DSS	603:605	arg1	colitis					607:613	acute and chronic DSS colitis	585:613	acute and chronic DSS colitis	585:613	Methods We studied the role of Casp11 in Il10-/- mice and acute and chronic DSS colitis mouse models.
30312415	5	15	theme	polymerase	680:689	arg1	reaction					697:704	quantative polymerase chain reaction	669:704	quantative polymerase chain reaction	669:704	We quantified colonic Casp11 mRNA using quantative polymerase chain reaction and colitis using weight loss, blinded histological scoring, IL-12/23p40 secretion by colonic explants, and fecal lipocalin-2.
30312415	13	16	theme	chronic	1553:1559	arg1	colitis					1574:1580	chronic experimental colitis	1553:1580	chronic experimental colitis	1553:1580	Conclusions Casp11 does not impact chronic experimental colitis, and its effects on acute DSS colitis vary with environmental factors including the microbiota, particularly Clostridiales.
30312415	5	17	theme	fecal	814:818	arg1	lipocalin-2					820:830	fecal lipocalin-2	814:830	fecal lipocalin-2	814:830	We quantified colonic Casp11 mRNA using quantative polymerase chain reaction and colitis using weight loss, blinded histological scoring, IL-12/23p40 secretion by colonic explants, and fecal lipocalin-2.
30312415	4	18	theme	mouse	615:619	arg1	models					621:626	mouse models	615:626	mouse models	615:626	Methods We studied the role of Casp11 in Il10-/- mice and acute and chronic DSS colitis mouse models.
30312415	9	19	theme	DSS	1208:1210	arg1	colitis					1212:1218	acute DSS colitis	1202:1218	acute DSS colitis	1202:1218	Contrary to prior reports, we initially observed that Casp11 exacerbates acute DSS colitis.
30312415	14	20	theme	acute	1855:1859	arg1	colitis					1865:1871	acute DSS colitis	1855:1871	acute DSS colitis models	1855:1878	Stochastic drifts in intestinal microbiota composition, even in mice in the same housing facility, should be considered when interpreting studies of acute DSS colitis models.
30312415	14	21	theme	housing	1787:1793	arg1	facility					1795:1802	the same housing facility	1778:1802	the same housing facility	1778:1802	Stochastic drifts in intestinal microbiota composition, even in mice in the same housing facility, should be considered when interpreting studies of acute DSS colitis models.
30312415	5	22	theme	Casp11	651:656	arg1	mRNA					658:661	colonic Casp11 mRNA	643:661	colonic Casp11 mRNA	643:661	We quantified colonic Casp11 mRNA using quantative polymerase chain reaction and colitis using weight loss, blinded histological scoring, IL-12/23p40 secretion by colonic explants, and fecal lipocalin-2.
30312415	7	23	with	mice	967:970	arg1	colitis					985:991	chronic colitis	977:991	chronic colitis	977:991	Results We detected increased colonic Casp11 mRNA in Il10-/- mice with chronic colitis, but not in mice with DSS colitis.
30312415	7	24	theme	DSS	1015:1017	arg1	colitis					1019:1025	DSS colitis	1015:1025	DSS colitis	1015:1025	Results We detected increased colonic Casp11 mRNA in Il10-/- mice with chronic colitis, but not in mice with DSS colitis.
30312415	2	25	theme	noncanonical	252:263	arg1	Casp11					300:305	Casp11	300:305	Casp11	300:305	The noncanonical inflammasome activator caspase-11 (Casp11) reportedly attenuates acute dextran sodium sulfate (DSS) colitis in mice.
30312415	2	25	theme	noncanonical	252:263	arg1	caspase-11					288:297	The noncanonical inflammasome activator caspase-11	248:297	The noncanonical inflammasome activator caspase-11 (Casp11)	248:306	The noncanonical inflammasome activator caspase-11 (Casp11) reportedly attenuates acute dextran sodium sulfate (DSS) colitis in mice.
30312415	0	26	theme	Colitis	55:61	arg1	Severity					33:40	the Severity	29:40	the Severity of Acute DSS Colitis in Caspase-11-Deficient Mice	29:90	Environmental Factors Modify the Severity of Acute DSS Colitis in Caspase-11-Deficient Mice.
30312415	4	27	dep	Methods	527:533	arg1	studied					538:544	studied	538:544	studied the role of Casp11 in Il10-/- mice and acute and chronic DSS colitis mouse models	538:626	Methods We studied the role of Casp11 in Il10-/- mice and acute and chronic DSS colitis mouse models.
30312415	0	28	theme	Caspase-11-Deficient	66:85	arg1	Mice					87:90	Caspase-11-Deficient Mice	66:90	Caspase-11-Deficient Mice	66:90	Environmental Factors Modify the Severity of Acute DSS Colitis in Caspase-11-Deficient Mice.
30312415	7	29	theme	Il10-/-	959:965	arg1	mice					967:970	Il10-/- mice	959:970	Il10-/- mice with chronic colitis	959:991	Results We detected increased colonic Casp11 mRNA in Il10-/- mice with chronic colitis, but not in mice with DSS colitis.
30312415	6	30	theme	microbial	853:861	arg1	composition					863:873	fecal microbial composition	847:873	fecal microbial composition	847:873	We determined fecal microbial composition using 16S amplicon sequencing.
30312415	4	31	dep	role	550:553	arg1	models					621:626	mouse models	615:626	mouse models	615:626	Methods We studied the role of Casp11 in Il10-/- mice and acute and chronic DSS colitis mouse models.
30312415	8	32	from	severity	1069:1076	arg1	mice					1123:1126	DSS-treated or Il10-/- mice	1100:1126	DSS-treated or Il10-/- mice	1100:1126	The presence of Casp11 did not alter the severity of chronic colitis in DSS-treated or Il10-/- mice.
30312415	13	33	theme	environmental	1630:1642	arg1	factors					1644:1650	environmental factors	1630:1650	environmental factors including the microbiota	1630:1675	Conclusions Casp11 does not impact chronic experimental colitis, and its effects on acute DSS colitis vary with environmental factors including the microbiota, particularly Clostridiales.
30312415	13	33	theme	environmental	1630:1642	arg1	microbiota					1666:1675	the microbiota	1662:1675	the microbiota	1662:1675	Conclusions Casp11 does not impact chronic experimental colitis, and its effects on acute DSS colitis vary with environmental factors including the microbiota, particularly Clostridiales.
30312415	13	33	theme	environmental	1630:1642	arg1	Clostridiales					1691:1703	Clostridiales	1691:1703	Clostridiales	1691:1703	Conclusions Casp11 does not impact chronic experimental colitis, and its effects on acute DSS colitis vary with environmental factors including the microbiota, particularly Clostridiales.
30312415	10	34	theme	animal	1256:1261	arg1	facility					1263:1270	the same animal facility	1247:1270	the same animal facility	1247:1270	Subsequent experiments in the same animal facility revealed no effect of Casp11 on acute DSS colitis.
30312415	3	35	theme	experimental	424:435	arg1	colitis					437:443	chronic experimental colitis	416:443	chronic experimental colitis	416:443	However, the effects of Casp11 on chronic experimental colitis and factors that influence the impact of Casp11 on acute DSS colitis are unknown.
30312415	5	36	theme	weight	724:729	arg1	loss					731:734	weight loss	724:734	weight loss	724:734	We quantified colonic Casp11 mRNA using quantative polymerase chain reaction and colitis using weight loss, blinded histological scoring, IL-12/23p40 secretion by colonic explants, and fecal lipocalin-2.
30312415	7	37	theme	colonic	936:942	arg1	mRNA					951:954	increased colonic Casp11 mRNA	926:954	increased colonic Casp11 mRNA	926:954	Results We detected increased colonic Casp11 mRNA in Il10-/- mice with chronic colitis, but not in mice with DSS colitis.
30312415	0	38	from	Severity	33:40	arg1	Mice					87:90	Caspase-11-Deficient Mice	66:90	Caspase-11-Deficient Mice	66:90	Environmental Factors Modify the Severity of Acute DSS Colitis in Caspase-11-Deficient Mice.
30312415	0	39	theme	Environmental	0:12	arg1	Factors					14:20	Environmental Factors	0:20	Environmental Factors	0:20	Environmental Factors Modify the Severity of Acute DSS Colitis in Caspase-11-Deficient Mice.
30312415	1	40	theme	Background	93:102	arg1	studies					120:126	Background Human and mouse studies	93:126	Background Human and mouse studies	93:126	Background Human and mouse studies implicate the inflammasome in the pathogenesis of inflammatory bowel diseases, though the effects in mice are variable.
30312415	13	41	theme	DSS	1608:1610	arg1	colitis					1612:1618	acute DSS colitis	1602:1618	acute DSS colitis	1602:1618	Conclusions Casp11 does not impact chronic experimental colitis, and its effects on acute DSS colitis vary with environmental factors including the microbiota, particularly Clostridiales.
30312415	14	42	theme	same	1782:1785	arg1	facility					1795:1802	the same housing facility	1778:1802	the same housing facility	1778:1802	Stochastic drifts in intestinal microbiota composition, even in mice in the same housing facility, should be considered when interpreting studies of acute DSS colitis models.
30312415	12	43	theme	wild-type	1461:1469	arg1	mice					1484:1487	wild-type vs Casp11-/- mice	1461:1487	wild-type vs Casp11-/- mice	1461:1487	The majority of bacterial taxa that changed over time in wild-type vs Casp11-/- mice belong to the Clostridiales.
30312415	12	44	theme	Casp11-/-	1474:1482	arg1	mice					1484:1487	wild-type vs Casp11-/- mice	1461:1487	wild-type vs Casp11-/- mice	1461:1487	The majority of bacterial taxa that changed over time in wild-type vs Casp11-/- mice belong to the Clostridiales.
30312415	10	45	theme	acute	1304:1308	arg1	colitis					1314:1320	acute DSS colitis	1304:1320	acute DSS colitis	1304:1320	Subsequent experiments in the same animal facility revealed no effect of Casp11 on acute DSS colitis.
30312415	0	46	theme	Acute	45:49	arg1	Colitis					55:61	Acute DSS Colitis	45:61	Acute DSS Colitis	45:61	Environmental Factors Modify the Severity of Acute DSS Colitis in Caspase-11-Deficient Mice.
30312415	10	47	theme	Casp11	1294:1299	arg1	effect					1284:1289	no effect	1281:1289	no effect of Casp11 on acute DSS colitis	1281:1320	Subsequent experiments in the same animal facility revealed no effect of Casp11 on acute DSS colitis.
30312415	2	48	theme	sodium	344:349	arg1	sulfate					351:357	dextran sodium sulfate	336:357	acute dextran sodium sulfate (DSS) colitis in mice	330:379	The noncanonical inflammasome activator caspase-11 (Casp11) reportedly attenuates acute dextran sodium sulfate (DSS) colitis in mice.
30312415	2	48	theme	sodium	344:349	arg1	DSS					360:362	DSS	360:362	DSS	360:362	The noncanonical inflammasome activator caspase-11 (Casp11) reportedly attenuates acute dextran sodium sulfate (DSS) colitis in mice.
30312415	8	49	theme	chronic	1081:1087	arg1	colitis					1089:1095	chronic colitis	1081:1095	chronic colitis	1081:1095	The presence of Casp11 did not alter the severity of chronic colitis in DSS-treated or Il10-/- mice.
30312415	3	50	theme	DSS	502:504	arg1	colitis					506:512	acute DSS colitis	496:512	acute DSS colitis	496:512	However, the effects of Casp11 on chronic experimental colitis and factors that influence the impact of Casp11 on acute DSS colitis are unknown.
30312415	14	51	theme	microbiota	1738:1747	arg1	composition					1749:1759	intestinal microbiota composition	1727:1759	intestinal microbiota composition	1727:1759	Stochastic drifts in intestinal microbiota composition, even in mice in the same housing facility, should be considered when interpreting studies of acute DSS colitis models.
30312415	0	52	theme	DSS	51:53	arg1	Colitis					55:61	Acute DSS Colitis	45:61	Acute DSS Colitis	45:61	Environmental Factors Modify the Severity of Acute DSS Colitis in Caspase-11-Deficient Mice.
30312415	4	53	theme	acute	585:589	arg1	colitis					607:613	acute and chronic DSS colitis	585:613	acute and chronic DSS colitis	585:613	Methods We studied the role of Casp11 in Il10-/- mice and acute and chronic DSS colitis mouse models.
30312415	11	54	theme	pronounced	1334:1343	arg1	changes					1356:1362	pronounced stochastic changes	1334:1362	pronounced stochastic changes	1334:1362	There were pronounced stochastic changes in the fecal microbiome over this time.
30312415	12	55	theme	taxa	1430:1433	arg1	majority					1408:1415	The majority	1404:1415	The majority of bacterial taxa that changed over time in wild-type vs Casp11-/- mice	1404:1487	The majority of bacterial taxa that changed over time in wild-type vs Casp11-/- mice belong to the Clostridiales.
30312415	13	56	theme	experimental	1561:1572	arg1	colitis					1574:1580	chronic experimental colitis	1553:1580	chronic experimental colitis	1553:1580	Conclusions Casp11 does not impact chronic experimental colitis, and its effects on acute DSS colitis vary with environmental factors including the microbiota, particularly Clostridiales.
30312415	14	57	theme	Stochastic	1706:1715	arg1	drifts					1717:1722	Stochastic drifts	1706:1722	Stochastic drifts	1706:1722	Stochastic drifts in intestinal microbiota composition, even in mice in the same housing facility, should be considered when interpreting studies of acute DSS colitis models.
30312415	6	58	theme	amplicon	885:892	arg1	sequencing					894:903	16S amplicon sequencing	881:903	16S amplicon sequencing	881:903	We determined fecal microbial composition using 16S amplicon sequencing.
30312415	14	59	from	mice	1770:1773	arg1	facility					1795:1802	the same housing facility	1778:1802	the same housing facility	1778:1802	Stochastic drifts in intestinal microbiota composition, even in mice in the same housing facility, should be considered when interpreting studies of acute DSS colitis models.
30312415	5	60	theme	histological	745:756	arg1	scoring					758:764	blinded histological scoring	737:764	blinded histological scoring	737:764	We quantified colonic Casp11 mRNA using quantative polymerase chain reaction and colitis using weight loss, blinded histological scoring, IL-12/23p40 secretion by colonic explants, and fecal lipocalin-2.
30312415	3	61	from	impact	476:481	arg1	colitis					506:512	acute DSS colitis	496:512	acute DSS colitis	496:512	However, the effects of Casp11 on chronic experimental colitis and factors that influence the impact of Casp11 on acute DSS colitis are unknown.
30312415	2	62	theme	acute	330:334	arg1	colitis					365:371	acute dextran sodium sulfate (DSS) colitis	330:371	acute dextran sodium sulfate (DSS) colitis in mice	330:379	The noncanonical inflammasome activator caspase-11 (Casp11) reportedly attenuates acute dextran sodium sulfate (DSS) colitis in mice.
30312415	1	63	theme	bowel	191:195	arg1	diseases					197:204	inflammatory bowel diseases	178:204	inflammatory bowel diseases	178:204	Background Human and mouse studies implicate the inflammasome in the pathogenesis of inflammatory bowel diseases, though the effects in mice are variable.
30312415	5	64	theme	quantative	669:678	arg1	reaction					697:704	quantative polymerase chain reaction	669:704	quantative polymerase chain reaction	669:704	We quantified colonic Casp11 mRNA using quantative polymerase chain reaction and colitis using weight loss, blinded histological scoring, IL-12/23p40 secretion by colonic explants, and fecal lipocalin-2.
30312415	4	65	from	colitis	607:613	arg1	mice					576:579	Il10-/- mice	568:579	Il10-/- mice	568:579	Methods We studied the role of Casp11 in Il10-/- mice and acute and chronic DSS colitis mouse models.
30312415	14	66	theme	models	1873:1878	arg1	studies					1844:1850	studies	1844:1850	studies of acute DSS colitis models	1844:1878	Stochastic drifts in intestinal microbiota composition, even in mice in the same housing facility, should be considered when interpreting studies of acute DSS colitis models.
30312415	10	67	from	experiments	1232:1242	arg1	facility					1263:1270	the same animal facility	1247:1270	the same animal facility	1247:1270	Subsequent experiments in the same animal facility revealed no effect of Casp11 on acute DSS colitis.
30312415	4	68	theme	chronic	595:601	arg1	colitis					607:613	acute and chronic DSS colitis	585:613	acute and chronic DSS colitis	585:613	Methods We studied the role of Casp11 in Il10-/- mice and acute and chronic DSS colitis mouse models.
30312415	2	69	theme	sulfate	351:357	arg1	colitis					365:371	acute dextran sodium sulfate (DSS) colitis	330:371	acute dextran sodium sulfate (DSS) colitis in mice	330:379	The noncanonical inflammasome activator caspase-11 (Casp11) reportedly attenuates acute dextran sodium sulfate (DSS) colitis in mice.
30312415	5	70	theme	chain	691:695	arg1	reaction					697:704	quantative polymerase chain reaction	669:704	quantative polymerase chain reaction	669:704	We quantified colonic Casp11 mRNA using quantative polymerase chain reaction and colitis using weight loss, blinded histological scoring, IL-12/23p40 secretion by colonic explants, and fecal lipocalin-2.
30312415	9	71	theme	acute	1202:1206	arg1	colitis					1212:1218	acute DSS colitis	1202:1218	acute DSS colitis	1202:1218	Contrary to prior reports, we initially observed that Casp11 exacerbates acute DSS colitis.
30312415	14	72	theme	DSS	1861:1863	arg1	colitis					1865:1871	acute DSS colitis	1855:1871	acute DSS colitis models	1855:1878	Stochastic drifts in intestinal microbiota composition, even in mice in the same housing facility, should be considered when interpreting studies of acute DSS colitis models.
30312415	5	73	theme	colonic	643:649	arg1	mRNA					658:661	colonic Casp11 mRNA	643:661	colonic Casp11 mRNA	643:661	We quantified colonic Casp11 mRNA using quantative polymerase chain reaction and colitis using weight loss, blinded histological scoring, IL-12/23p40 secretion by colonic explants, and fecal lipocalin-2.
30312415	13	74	theme	Conclusions	1518:1528	arg1	Casp11					1530:1535	Conclusions Casp11	1518:1535	Conclusions Casp11	1518:1535	Conclusions Casp11 does not impact chronic experimental colitis, and its effects on acute DSS colitis vary with environmental factors including the microbiota, particularly Clostridiales.
30312415	1	75	from	effects	218:224	arg1	mice					229:232	mice	229:232	mice	229:232	Background Human and mouse studies implicate the inflammasome in the pathogenesis of inflammatory bowel diseases, though the effects in mice are variable.
30312415	1	76	theme	mouse	114:118	arg1	studies					120:126	Background Human and mouse studies	93:126	Background Human and mouse studies	93:126	Background Human and mouse studies implicate the inflammasome in the pathogenesis of inflammatory bowel diseases, though the effects in mice are variable.
30312415	2	77	theme	inflammasome	265:276	arg1	Casp11					300:305	Casp11	300:305	Casp11	300:305	The noncanonical inflammasome activator caspase-11 (Casp11) reportedly attenuates acute dextran sodium sulfate (DSS) colitis in mice.
30312415	2	77	theme	inflammasome	265:276	arg1	caspase-11					288:297	The noncanonical inflammasome activator caspase-11	248:297	The noncanonical inflammasome activator caspase-11 (Casp11)	248:306	The noncanonical inflammasome activator caspase-11 (Casp11) reportedly attenuates acute dextran sodium sulfate (DSS) colitis in mice.
30312415	10	78	from	effect	1284:1289	arg1	colitis					1314:1320	acute DSS colitis	1304:1320	acute DSS colitis	1304:1320	Subsequent experiments in the same animal facility revealed no effect of Casp11 on acute DSS colitis.
30312415	5	79	theme	blinded	737:743	arg1	scoring					758:764	blinded histological scoring	737:764	blinded histological scoring	737:764	We quantified colonic Casp11 mRNA using quantative polymerase chain reaction and colitis using weight loss, blinded histological scoring, IL-12/23p40 secretion by colonic explants, and fecal lipocalin-2.
30312415	3	80	from	effects	395:401	arg1	factors					449:455	factors	449:455	factors that influence the impact of Casp11 on acute DSS colitis	449:512	However, the effects of Casp11 on chronic experimental colitis and factors that influence the impact of Casp11 on acute DSS colitis are unknown.
30312415	3	80	from	effects	395:401	arg1	colitis					437:443	chronic experimental colitis	416:443	chronic experimental colitis	416:443	However, the effects of Casp11 on chronic experimental colitis and factors that influence the impact of Casp11 on acute DSS colitis are unknown.
30312415	7	81	theme	chronic	977:983	arg1	colitis					985:991	chronic colitis	977:991	chronic colitis	977:991	Results We detected increased colonic Casp11 mRNA in Il10-/- mice with chronic colitis, but not in mice with DSS colitis.
30312415	10	82	theme	Subsequent	1221:1230	arg1	experiments					1232:1242	Subsequent experiments	1221:1242	Subsequent experiments in the same animal facility	1221:1270	Subsequent experiments in the same animal facility revealed no effect of Casp11 on acute DSS colitis.
30312415	14	83	theme	colitis	1865:1871	arg1	models					1873:1878	acute DSS colitis models	1855:1878	acute DSS colitis models	1855:1878	Stochastic drifts in intestinal microbiota composition, even in mice in the same housing facility, should be considered when interpreting studies of acute DSS colitis models.
30312415	7	84	with	mice	1005:1008	arg1	colitis					1019:1025	DSS colitis	1015:1025	DSS colitis	1015:1025	Results We detected increased colonic Casp11 mRNA in Il10-/- mice with chronic colitis, but not in mice with DSS colitis.
30312415	10	85	theme	same	1251:1254	arg1	facility					1263:1270	the same animal facility	1247:1270	the same animal facility	1247:1270	Subsequent experiments in the same animal facility revealed no effect of Casp11 on acute DSS colitis.
30312415	4	86	from	role	550:553	arg1	mice					576:579	Il10-/- mice	568:579	Il10-/- mice	568:579	Methods We studied the role of Casp11 in Il10-/- mice and acute and chronic DSS colitis mouse models.
30312415	8	87	theme	Casp11	1044:1049	arg1	presence					1032:1039	The presence	1028:1039	The presence of Casp11	1028:1049	The presence of Casp11 did not alter the severity of chronic colitis in DSS-treated or Il10-/- mice.
30312415	7	88	theme	Casp11	944:949	arg1	mRNA					951:954	increased colonic Casp11 mRNA	926:954	increased colonic Casp11 mRNA	926:954	Results We detected increased colonic Casp11 mRNA in Il10-/- mice with chronic colitis, but not in mice with DSS colitis.
30312415	3	89	theme	chronic	416:422	arg1	colitis					437:443	chronic experimental colitis	416:443	chronic experimental colitis	416:443	However, the effects of Casp11 on chronic experimental colitis and factors that influence the impact of Casp11 on acute DSS colitis are unknown.
30312415	5	90	theme	colonic	792:798	arg1	explants					800:807	colonic explants	792:807	colonic explants	792:807	We quantified colonic Casp11 mRNA using quantative polymerase chain reaction and colitis using weight loss, blinded histological scoring, IL-12/23p40 secretion by colonic explants, and fecal lipocalin-2.
30312415	7	91	theme	increased	926:934	arg1	mRNA					951:954	increased colonic Casp11 mRNA	926:954	increased colonic Casp11 mRNA	926:954	Results We detected increased colonic Casp11 mRNA in Il10-/- mice with chronic colitis, but not in mice with DSS colitis.
30312415	1	92	theme	Human	104:108	arg1	studies					120:126	Background Human and mouse studies	93:126	Background Human and mouse studies	93:126	Background Human and mouse studies implicate the inflammasome in the pathogenesis of inflammatory bowel diseases, though the effects in mice are variable.
30312415	6	93	theme	fecal	847:851	arg1	composition					863:873	fecal microbial composition	847:873	fecal microbial composition	847:873	We determined fecal microbial composition using 16S amplicon sequencing.
30312415	3	94	theme	Casp11	406:411	arg1	effects					395:401	the effects	391:401	the effects of Casp11 on chronic experimental colitis and factors that influence the impact of Casp11 on acute DSS colitis	391:512	However, the effects of Casp11 on chronic experimental colitis and factors that influence the impact of Casp11 on acute DSS colitis are unknown.
30312415	3	94	theme	Casp11	406:411	arg1	unknown					518:524	unknown	518:524	unknown	518:524	However, the effects of Casp11 on chronic experimental colitis and factors that influence the impact of Casp11 on acute DSS colitis are unknown.
30312415	10	95	theme	DSS	1310:1312	arg1	colitis					1314:1320	acute DSS colitis	1304:1320	acute DSS colitis	1304:1320	Subsequent experiments in the same animal facility revealed no effect of Casp11 on acute DSS colitis.
30312415	14	96	from	drifts	1717:1722	arg1	mice					1770:1773	mice	1770:1773	mice in the same housing facility	1770:1802	Stochastic drifts in intestinal microbiota composition, even in mice in the same housing facility, should be considered when interpreting studies of acute DSS colitis models.
30312415	14	96	from	drifts	1717:1722	arg1	composition					1749:1759	intestinal microbiota composition	1727:1759	intestinal microbiota composition	1727:1759	Stochastic drifts in intestinal microbiota composition, even in mice in the same housing facility, should be considered when interpreting studies of acute DSS colitis models.
30312415	5	97	theme	IL-12/23p40	767:777	arg1	secretion					779:787	IL-12/23p40 secretion	767:787	IL-12/23p40 secretion by colonic explants	767:807	We quantified colonic Casp11 mRNA using quantative polymerase chain reaction and colitis using weight loss, blinded histological scoring, IL-12/23p40 secretion by colonic explants, and fecal lipocalin-2.
30312415	3	98	theme	Casp11	486:491	arg1	impact					476:481	the impact	472:481	the impact of Casp11 on acute DSS colitis	472:512	However, the effects of Casp11 on chronic experimental colitis and factors that influence the impact of Casp11 on acute DSS colitis are unknown.
30312415	8	99	theme	colitis	1089:1095	arg1	severity					1069:1076	the severity	1065:1076	the severity of chronic colitis in DSS-treated or Il10-/- mice	1065:1126	The presence of Casp11 did not alter the severity of chronic colitis in DSS-treated or Il10-/- mice.
30312415	13	100	theme	acute	1602:1606	arg1	colitis					1612:1618	acute DSS colitis	1602:1618	acute DSS colitis	1602:1618	Conclusions Casp11 does not impact chronic experimental colitis, and its effects on acute DSS colitis vary with environmental factors including the microbiota, particularly Clostridiales.
30312415	2	101	theme	dextran	336:342	arg1	sulfate					351:357	dextran sodium sulfate	336:357	acute dextran sodium sulfate (DSS) colitis in mice	330:379	The noncanonical inflammasome activator caspase-11 (Casp11) reportedly attenuates acute dextran sodium sulfate (DSS) colitis in mice.
30312415	2	101	theme	dextran	336:342	arg1	DSS					360:362	DSS	360:362	DSS	360:362	The noncanonical inflammasome activator caspase-11 (Casp11) reportedly attenuates acute dextran sodium sulfate (DSS) colitis in mice.
30312415	8	102	theme	DSS-treated	1100:1110	arg1	mice					1123:1126	DSS-treated or Il10-/- mice	1100:1126	DSS-treated or Il10-/- mice	1100:1126	The presence of Casp11 did not alter the severity of chronic colitis in DSS-treated or Il10-/- mice.
30312415	14	103	theme	intestinal	1727:1736	arg1	composition					1749:1759	intestinal microbiota composition	1727:1759	intestinal microbiota composition	1727:1759	Stochastic drifts in intestinal microbiota composition, even in mice in the same housing facility, should be considered when interpreting studies of acute DSS colitis models.
30312415	13	104	from	effects	1591:1597	arg1	colitis					1612:1618	acute DSS colitis	1602:1618	acute DSS colitis	1602:1618	Conclusions Casp11 does not impact chronic experimental colitis, and its effects on acute DSS colitis vary with environmental factors including the microbiota, particularly Clostridiales.
29525100	9	0	theme	inflammatory	1719:1730	arg1	reaction					1732:1739	any significant inflammatory reaction	1703:1739	any significant inflammatory reaction	1703:1739	In vivo implantation of GCT30 scaffold subcutaneously into mice did not indicate any significant inflammatory reaction, but ongoing vascularization.
29525100	6	1	theme	scaffolds	1143:1151	arg1	strength					1127:1134	Compressive strength	1115:1134	Compressive strength of the scaffolds	1115:1151	Compressive strength of the scaffolds was increased from 0.8 MPa to 2.45 MPa on increase in β-TCP content from 10 wt%-30 wt% in the prepared scaffold.
29525100	1	2	theme	degradation	296:306	arg1	rate					308:311	predictable degradation rate	284:311	predictable degradation rate	284:311	The primary aim of this study was to fabricate gelatin/chitosan/β-TCP (GCT) composite scaffold to improve its compressive mechanical behaviour and in-vivo biocompatibility with predictable degradation rate.
29525100	6	3	theme	%	1231:1231	arg1	%					1238:1238	10 wt%-30 wt%	1226:1238	10 wt%-30 wt% in the prepared scaffold	1226:1263	Compressive strength of the scaffolds was increased from 0.8 MPa to 2.45 MPa on increase in β-TCP content from 10 wt%-30 wt% in the prepared scaffold.
29525100	3	4	theme	phase	770:774	arg1	mixture					786:792	the phase separated mixture	766:792	the phase separated mixture	766:792	The composite scaffolds were fabricated using solid-liquid phase separation of the slurry containing gelatin, chitosan, β-tricalcium phosphate in varying proportion and subsequent lyophilisation of the phase separated mixture.
29525100	2	5	theme	phosphate	330:338	arg1	powder					348:353	Beta tricalcium phosphate (β-TCP) powder	314:353	Beta tricalcium phosphate (β-TCP) powder	314:353	Beta tricalcium phosphate (β-TCP) powder was synthesized in size range between 70-100 nm using aqueous precipitation route at a fixed Ca/P molar ratio of 1.5:1 at pH 10 and after subsequent heat treatment of as precipitated powder at 800 °C for 4 hours.
29525100	2	6	theme	Beta	314:317	arg1	powder					348:353	Beta tricalcium phosphate (β-TCP) powder	314:353	Beta tricalcium phosphate (β-TCP) powder	314:353	Beta tricalcium phosphate (β-TCP) powder was synthesized in size range between 70-100 nm using aqueous precipitation route at a fixed Ca/P molar ratio of 1.5:1 at pH 10 and after subsequent heat treatment of as precipitated powder at 800 °C for 4 hours.
29525100	6	7	theme	β-TCP	1207:1211	arg1	content					1213:1219	β-TCP content	1207:1219	β-TCP content from 10 wt%-30 wt% in the prepared scaffold	1207:1263	Compressive strength of the scaffolds was increased from 0.8 MPa to 2.45 MPa on increase in β-TCP content from 10 wt%-30 wt% in the prepared scaffold.
29525100	4	8	with	porosity	833:840	arg1	sizes					859:863	pore sizes	854:863	pore sizes ranging between 78-382 μm	854:889	The prepared scaffolds exhibited high porosity (>80%) with pore sizes ranging between 78-382 μm as determined using Hg-porosimetry.
29525100	5	9	theme	SEM	927:929	arg1	result					931:936	SEM result	927:936	SEM result	927:936	SEM result revealed that incorporation of β-TCP to the extent of 30 wt% resulted in well-shaped and uniformly distributed interconnected pores of average pore size of 120 ± 18.6 μm in it.
29525100	3	10	theme	mixture	786:792	arg1	chitosan					678:685	chitosan	678:685	chitosan	678:685	The composite scaffolds were fabricated using solid-liquid phase separation of the slurry containing gelatin, chitosan, β-tricalcium phosphate in varying proportion and subsequent lyophilisation of the phase separated mixture.
29525100	3	10	theme	mixture	786:792	arg1	phosphate					701:709	β-tricalcium phosphate	688:709	β-tricalcium phosphate in varying proportion	688:731	The composite scaffolds were fabricated using solid-liquid phase separation of the slurry containing gelatin, chitosan, β-tricalcium phosphate in varying proportion and subsequent lyophilisation of the phase separated mixture.
29525100	3	10	theme	mixture	786:792	arg1	gelatin					669:675	gelatin	669:675	gelatin	669:675	The composite scaffolds were fabricated using solid-liquid phase separation of the slurry containing gelatin, chitosan, β-tricalcium phosphate in varying proportion and subsequent lyophilisation of the phase separated mixture.
29525100	3	10	theme	mixture	786:792	arg1	lyophilisation					748:761	subsequent lyophilisation	737:761	subsequent lyophilisation of the phase separated mixture	737:792	The composite scaffolds were fabricated using solid-liquid phase separation of the slurry containing gelatin, chitosan, β-tricalcium phosphate in varying proportion and subsequent lyophilisation of the phase separated mixture.
29525100	2	11	theme	β-TCP	341:345	arg1	powder					348:353	Beta tricalcium phosphate (β-TCP) powder	314:353	Beta tricalcium phosphate (β-TCP) powder	314:353	Beta tricalcium phosphate (β-TCP) powder was synthesized in size range between 70-100 nm using aqueous precipitation route at a fixed Ca/P molar ratio of 1.5:1 at pH 10 and after subsequent heat treatment of as precipitated powder at 800 °C for 4 hours.
29525100	1	12	theme	compressive	217:227	arg1	behaviour					240:248	its compressive mechanical behaviour	213:248	its compressive mechanical behaviour	213:248	The primary aim of this study was to fabricate gelatin/chitosan/β-TCP (GCT) composite scaffold to improve its compressive mechanical behaviour and in-vivo biocompatibility with predictable degradation rate.
29525100	7	13	link	derived	1287:1293	arg1	cells					1312:1316	Human Umbilical Cord derived mesenchymal stem cells	1266:1316	Human Umbilical Cord derived mesenchymal stem cells (MSCs)	1266:1323	Human Umbilical Cord derived mesenchymal stem cells (MSCs) exhibited higher degree of lamellopodia and fillopodia extensions and better spreading behaviour onto GCT30 scaffold.
29525100	7	13	link	derived	1287:1293	arg1	MSCs					1319:1322	MSCs	1319:1322	MSCs	1319:1322	Human Umbilical Cord derived mesenchymal stem cells (MSCs) exhibited higher degree of lamellopodia and fillopodia extensions and better spreading behaviour onto GCT30 scaffold.
29525100	9	14	dep	In	1622:1623	arg1	vivo					1625:1628	vivo	1625:1628	vivo	1625:1628	In vivo implantation of GCT30 scaffold subcutaneously into mice did not indicate any significant inflammatory reaction, but ongoing vascularization.
29525100	3	15	from	lyophilisation	748:761	arg1	proportion					722:731	varying proportion	714:731	varying proportion	714:731	The composite scaffolds were fabricated using solid-liquid phase separation of the slurry containing gelatin, chitosan, β-tricalcium phosphate in varying proportion and subsequent lyophilisation of the phase separated mixture.
29525100	8	16	with	studies	1477:1483	arg1	MSCs					1499:1502	cultured MSCs	1490:1502	cultured MSCs	1490:1502	MTT assay and immunocytochemistry studies with cultured MSCs revealed that GCT30 scaffolds were more conducive to MSC's proliferation and differentiation into osteoblast lineage.
29525100	3	17	theme	solid-liquid	614:625	arg1	separation					633:642	solid-liquid phase separation	614:642	solid-liquid phase separation of the slurry containing gelatin, chitosan, β-tricalcium phosphate in varying proportion and subsequent lyophilisation of the phase separated mixture	614:792	The composite scaffolds were fabricated using solid-liquid phase separation of the slurry containing gelatin, chitosan, β-tricalcium phosphate in varying proportion and subsequent lyophilisation of the phase separated mixture.
29525100	5	18	theme	β-TCP	969:973	arg1	incorporation					952:964	incorporation	952:964	incorporation of β-TCP to the extent of 30 wt%	952:997	SEM result revealed that incorporation of β-TCP to the extent of 30 wt% resulted in well-shaped and uniformly distributed interconnected pores of average pore size of 120 ± 18.6 μm in it.
29525100	7	19	theme	better	1395:1400	arg1	behaviour					1412:1420	better spreading behaviour	1395:1420	better spreading behaviour	1395:1420	Human Umbilical Cord derived mesenchymal stem cells (MSCs) exhibited higher degree of lamellopodia and fillopodia extensions and better spreading behaviour onto GCT30 scaffold.
29525100	2	20	theme	precipitated	525:536	arg1	powder					538:543	as precipitated powder	522:543	as precipitated powder	522:543	Beta tricalcium phosphate (β-TCP) powder was synthesized in size range between 70-100 nm using aqueous precipitation route at a fixed Ca/P molar ratio of 1.5:1 at pH 10 and after subsequent heat treatment of as precipitated powder at 800 °C for 4 hours.
29525100	2	21	from	800 °C	548:553	arg1	ratio					459:463	a fixed Ca/P molar ratio	440:463	a fixed Ca/P molar ratio of 1.5:1 at pH 10	440:481	Beta tricalcium phosphate (β-TCP) powder was synthesized in size range between 70-100 nm using aqueous precipitation route at a fixed Ca/P molar ratio of 1.5:1 at pH 10 and after subsequent heat treatment of as precipitated powder at 800 °C for 4 hours.
29525100	5	22	from	it	1111:1112	arg1	size					1086:1089	average pore size	1073:1089	average pore size of 120 ± 18.6 μm in it	1073:1112	SEM result revealed that incorporation of β-TCP to the extent of 30 wt% resulted in well-shaped and uniformly distributed interconnected pores of average pore size of 120 ± 18.6 μm in it.
29525100	5	23	from	120 ± 18.6 μm	1094:1106	arg1	it					1111:1112	it	1111:1112	it	1111:1112	SEM result revealed that incorporation of β-TCP to the extent of 30 wt% resulted in well-shaped and uniformly distributed interconnected pores of average pore size of 120 ± 18.6 μm in it.
29525100	9	24	theme	scaffold	1652:1659	arg1	implantation					1630:1641	In vivo implantation	1622:1641	In vivo implantation of GCT30 scaffold subcutaneously into mice	1622:1684	In vivo implantation of GCT30 scaffold subcutaneously into mice did not indicate any significant inflammatory reaction, but ongoing vascularization.
29525100	7	25	theme	fillopodia	1369:1378	arg1	degree					1342:1347	higher degree	1335:1347	higher degree of lamellopodia and fillopodia extensions	1335:1389	Human Umbilical Cord derived mesenchymal stem cells (MSCs) exhibited higher degree of lamellopodia and fillopodia extensions and better spreading behaviour onto GCT30 scaffold.
29525100	7	25	theme	fillopodia	1369:1378	arg1	behaviour					1412:1420	better spreading behaviour	1395:1420	better spreading behaviour	1395:1420	Human Umbilical Cord derived mesenchymal stem cells (MSCs) exhibited higher degree of lamellopodia and fillopodia extensions and better spreading behaviour onto GCT30 scaffold.
29525100	7	26	theme	lamellopodia	1352:1363	arg1	degree					1342:1347	higher degree	1335:1347	higher degree of lamellopodia and fillopodia extensions	1335:1389	Human Umbilical Cord derived mesenchymal stem cells (MSCs) exhibited higher degree of lamellopodia and fillopodia extensions and better spreading behaviour onto GCT30 scaffold.
29525100	7	26	theme	lamellopodia	1352:1363	arg1	behaviour					1412:1420	better spreading behaviour	1395:1420	better spreading behaviour	1395:1420	Human Umbilical Cord derived mesenchymal stem cells (MSCs) exhibited higher degree of lamellopodia and fillopodia extensions and better spreading behaviour onto GCT30 scaffold.
29525100	2	27	theme	1.5:1	468:472	arg1	ratio					459:463	a fixed Ca/P molar ratio	440:463	a fixed Ca/P molar ratio of 1.5:1 at pH 10	440:481	Beta tricalcium phosphate (β-TCP) powder was synthesized in size range between 70-100 nm using aqueous precipitation route at a fixed Ca/P molar ratio of 1.5:1 at pH 10 and after subsequent heat treatment of as precipitated powder at 800 °C for 4 hours.
29525100	4	28	theme	prepared	799:806	arg1	scaffolds					808:816	The prepared scaffolds	795:816	The prepared scaffolds	795:816	The prepared scaffolds exhibited high porosity (>80%) with pore sizes ranging between 78-382 μm as determined using Hg-porosimetry.
29525100	8	29	theme	MTT	1443:1445	arg1	assay					1447:1451	MTT assay	1443:1451	MTT assay	1443:1451	MTT assay and immunocytochemistry studies with cultured MSCs revealed that GCT30 scaffolds were more conducive to MSC's proliferation and differentiation into osteoblast lineage.
29525100	1	30	with	behaviour	240:248	arg1	rate					308:311	predictable degradation rate	284:311	predictable degradation rate	284:311	The primary aim of this study was to fabricate gelatin/chitosan/β-TCP (GCT) composite scaffold to improve its compressive mechanical behaviour and in-vivo biocompatibility with predictable degradation rate.
29525100	5	31	theme	%	997:997	arg1	extent					982:987	the extent	978:987	the extent of 30 wt%	978:997	SEM result revealed that incorporation of β-TCP to the extent of 30 wt% resulted in well-shaped and uniformly distributed interconnected pores of average pore size of 120 ± 18.6 μm in it.
29525100	2	32	theme	heat	504:507	arg1	treatment					509:517	subsequent heat treatment	493:517	subsequent heat treatment of as precipitated powder	493:543	Beta tricalcium phosphate (β-TCP) powder was synthesized in size range between 70-100 nm using aqueous precipitation route at a fixed Ca/P molar ratio of 1.5:1 at pH 10 and after subsequent heat treatment of as precipitated powder at 800 °C for 4 hours.
29525100	0	33	theme	orthopaedic	82:92	arg1	application					94:104	orthopaedic application	82:104	orthopaedic application	82:104	Preparation and characterization of gelatin-chitosan-nanoβ-TCP based scaffold for orthopaedic application.
29525100	4	34	theme	pore	854:857	arg1	sizes					859:863	pore sizes	854:863	pore sizes ranging between 78-382 μm	854:889	The prepared scaffolds exhibited high porosity (>80%) with pore sizes ranging between 78-382 μm as determined using Hg-porosimetry.
29525100	2	35	theme	Ca/P	448:451	arg1	ratio					459:463	a fixed Ca/P molar ratio	440:463	a fixed Ca/P molar ratio of 1.5:1 at pH 10	440:481	Beta tricalcium phosphate (β-TCP) powder was synthesized in size range between 70-100 nm using aqueous precipitation route at a fixed Ca/P molar ratio of 1.5:1 at pH 10 and after subsequent heat treatment of as precipitated powder at 800 °C for 4 hours.
29525100	0	36	theme	gelatin-chitosan-nanoβ-TCP	36:61	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of gelatin-chitosan-nanoβ-TCP based scaffold for orthopaedic application.
29525100	0	36	theme	gelatin-chitosan-nanoβ-TCP	36:61	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of gelatin-chitosan-nanoβ-TCP based scaffold for orthopaedic application.
29525100	5	37	theme	average	1073:1079	arg1	size					1086:1089	average pore size	1073:1089	average pore size of 120 ± 18.6 μm in it	1073:1112	SEM result revealed that incorporation of β-TCP to the extent of 30 wt% resulted in well-shaped and uniformly distributed interconnected pores of average pore size of 120 ± 18.6 μm in it.
29525100	7	38	theme	Human	1266:1270	arg1	cells					1312:1316	Human Umbilical Cord derived mesenchymal stem cells	1266:1316	Human Umbilical Cord derived mesenchymal stem cells (MSCs)	1266:1323	Human Umbilical Cord derived mesenchymal stem cells (MSCs) exhibited higher degree of lamellopodia and fillopodia extensions and better spreading behaviour onto GCT30 scaffold.
29525100	7	38	theme	Human	1266:1270	arg1	MSCs					1319:1322	MSCs	1319:1322	MSCs	1319:1322	Human Umbilical Cord derived mesenchymal stem cells (MSCs) exhibited higher degree of lamellopodia and fillopodia extensions and better spreading behaviour onto GCT30 scaffold.
29525100	5	39	theme	size	1086:1089	arg1	pores					1064:1068	well-shaped and uniformly distributed interconnected pores	1011:1068	well-shaped and uniformly distributed interconnected pores of average pore size of 120 ± 18.6 μm in it	1011:1112	SEM result revealed that incorporation of β-TCP to the extent of 30 wt% resulted in well-shaped and uniformly distributed interconnected pores of average pore size of 120 ± 18.6 μm in it.
29525100	3	40	theme	varying	714:720	arg1	proportion					722:731	varying proportion	714:731	varying proportion	714:731	The composite scaffolds were fabricated using solid-liquid phase separation of the slurry containing gelatin, chitosan, β-tricalcium phosphate in varying proportion and subsequent lyophilisation of the phase separated mixture.
29525100	7	41	dep	lamellopodia	1352:1363	arg1	extensions					1380:1389	extensions	1380:1389	extensions	1380:1389	Human Umbilical Cord derived mesenchymal stem cells (MSCs) exhibited higher degree of lamellopodia and fillopodia extensions and better spreading behaviour onto GCT30 scaffold.
29525100	5	42	theme	distributed	1037:1047	arg1	pores					1064:1068	well-shaped and uniformly distributed interconnected pores	1011:1068	well-shaped and uniformly distributed interconnected pores of average pore size of 120 ± 18.6 μm in it	1011:1112	SEM result revealed that incorporation of β-TCP to the extent of 30 wt% resulted in well-shaped and uniformly distributed interconnected pores of average pore size of 120 ± 18.6 μm in it.
29525100	1	43	with	biocompatibility	262:277	arg1	rate					308:311	predictable degradation rate	284:311	predictable degradation rate	284:311	The primary aim of this study was to fabricate gelatin/chitosan/β-TCP (GCT) composite scaffold to improve its compressive mechanical behaviour and in-vivo biocompatibility with predictable degradation rate.
29525100	8	44	theme	cultured	1490:1497	arg1	MSCs					1499:1502	cultured MSCs	1490:1502	cultured MSCs	1490:1502	MTT assay and immunocytochemistry studies with cultured MSCs revealed that GCT30 scaffolds were more conducive to MSC's proliferation and differentiation into osteoblast lineage.
29525100	2	45	theme	aqueous	409:415	arg1	route					431:435	aqueous precipitation route	409:435	aqueous precipitation route	409:435	Beta tricalcium phosphate (β-TCP) powder was synthesized in size range between 70-100 nm using aqueous precipitation route at a fixed Ca/P molar ratio of 1.5:1 at pH 10 and after subsequent heat treatment of as precipitated powder at 800 °C for 4 hours.
29525100	9	46	theme	significant	1707:1717	arg1	reaction					1732:1739	any significant inflammatory reaction	1703:1739	any significant inflammatory reaction	1703:1739	In vivo implantation of GCT30 scaffold subcutaneously into mice did not indicate any significant inflammatory reaction, but ongoing vascularization.
29525100	7	47	theme	mesenchymal	1295:1305	arg1	cells					1312:1316	Human Umbilical Cord derived mesenchymal stem cells	1266:1316	Human Umbilical Cord derived mesenchymal stem cells (MSCs)	1266:1323	Human Umbilical Cord derived mesenchymal stem cells (MSCs) exhibited higher degree of lamellopodia and fillopodia extensions and better spreading behaviour onto GCT30 scaffold.
29525100	7	47	theme	mesenchymal	1295:1305	arg1	MSCs					1319:1322	MSCs	1319:1322	MSCs	1319:1322	Human Umbilical Cord derived mesenchymal stem cells (MSCs) exhibited higher degree of lamellopodia and fillopodia extensions and better spreading behaviour onto GCT30 scaffold.
29525100	1	48	theme	predictable	284:294	arg1	rate					308:311	predictable degradation rate	284:311	predictable degradation rate	284:311	The primary aim of this study was to fabricate gelatin/chitosan/β-TCP (GCT) composite scaffold to improve its compressive mechanical behaviour and in-vivo biocompatibility with predictable degradation rate.
29525100	7	49	theme	Cord	1282:1285	arg1	cells					1312:1316	Human Umbilical Cord derived mesenchymal stem cells	1266:1316	Human Umbilical Cord derived mesenchymal stem cells (MSCs)	1266:1323	Human Umbilical Cord derived mesenchymal stem cells (MSCs) exhibited higher degree of lamellopodia and fillopodia extensions and better spreading behaviour onto GCT30 scaffold.
29525100	7	49	theme	Cord	1282:1285	arg1	MSCs					1319:1322	MSCs	1319:1322	MSCs	1319:1322	Human Umbilical Cord derived mesenchymal stem cells (MSCs) exhibited higher degree of lamellopodia and fillopodia extensions and better spreading behaviour onto GCT30 scaffold.
29525100	3	50	from	gelatin	669:675	arg1	proportion					722:731	varying proportion	714:731	varying proportion	714:731	The composite scaffolds were fabricated using solid-liquid phase separation of the slurry containing gelatin, chitosan, β-tricalcium phosphate in varying proportion and subsequent lyophilisation of the phase separated mixture.
29525100	6	51	theme	10 wt	1226:1230	arg1	%					1238:1238	10 wt%-30 wt%	1226:1238	10 wt%-30 wt% in the prepared scaffold	1226:1263	Compressive strength of the scaffolds was increased from 0.8 MPa to 2.45 MPa on increase in β-TCP content from 10 wt%-30 wt% in the prepared scaffold.
29525100	3	52	from	chitosan	678:685	arg1	proportion					722:731	varying proportion	714:731	varying proportion	714:731	The composite scaffolds were fabricated using solid-liquid phase separation of the slurry containing gelatin, chitosan, β-tricalcium phosphate in varying proportion and subsequent lyophilisation of the phase separated mixture.
29525100	3	53	theme	separated	776:784	arg1	mixture					786:792	the phase separated mixture	766:792	the phase separated mixture	766:792	The composite scaffolds were fabricated using solid-liquid phase separation of the slurry containing gelatin, chitosan, β-tricalcium phosphate in varying proportion and subsequent lyophilisation of the phase separated mixture.
29525100	2	54	theme	tricalcium	319:328	arg1	powder					348:353	Beta tricalcium phosphate (β-TCP) powder	314:353	Beta tricalcium phosphate (β-TCP) powder	314:353	Beta tricalcium phosphate (β-TCP) powder was synthesized in size range between 70-100 nm using aqueous precipitation route at a fixed Ca/P molar ratio of 1.5:1 at pH 10 and after subsequent heat treatment of as precipitated powder at 800 °C for 4 hours.
29525100	1	55	theme	composite	183:191	arg1	scaffold					193:200	composite scaffold	183:200	composite scaffold	183:200	The primary aim of this study was to fabricate gelatin/chitosan/β-TCP (GCT) composite scaffold to improve its compressive mechanical behaviour and in-vivo biocompatibility with predictable degradation rate.
29525100	3	56	theme	composite	572:580	arg1	scaffolds					582:590	The composite scaffolds	568:590	The composite scaffolds	568:590	The composite scaffolds were fabricated using solid-liquid phase separation of the slurry containing gelatin, chitosan, β-tricalcium phosphate in varying proportion and subsequent lyophilisation of the phase separated mixture.
29525100	5	57	from	size	1086:1089	arg1	it					1111:1112	it	1111:1112	it	1111:1112	SEM result revealed that incorporation of β-TCP to the extent of 30 wt% resulted in well-shaped and uniformly distributed interconnected pores of average pore size of 120 ± 18.6 μm in it.
29525100	3	58	theme	phase	627:631	arg1	separation					633:642	solid-liquid phase separation	614:642	solid-liquid phase separation of the slurry containing gelatin, chitosan, β-tricalcium phosphate in varying proportion and subsequent lyophilisation of the phase separated mixture	614:792	The composite scaffolds were fabricated using solid-liquid phase separation of the slurry containing gelatin, chitosan, β-tricalcium phosphate in varying proportion and subsequent lyophilisation of the phase separated mixture.
29525100	1	59	theme	mechanical	229:238	arg1	behaviour					240:248	its compressive mechanical behaviour	213:248	its compressive mechanical behaviour	213:248	The primary aim of this study was to fabricate gelatin/chitosan/β-TCP (GCT) composite scaffold to improve its compressive mechanical behaviour and in-vivo biocompatibility with predictable degradation rate.
29525100	8	60	theme	GCT30	1518:1522	arg1	scaffolds					1524:1532	GCT30 scaffolds	1518:1532	GCT30 scaffolds	1518:1532	MTT assay and immunocytochemistry studies with cultured MSCs revealed that GCT30 scaffolds were more conducive to MSC's proliferation and differentiation into osteoblast lineage.
29525100	1	61	theme	study	131:135	arg1	aim					119:121	The primary aim	107:121	The primary aim of this study	107:135	The primary aim of this study was to fabricate gelatin/chitosan/β-TCP (GCT) composite scaffold to improve its compressive mechanical behaviour and in-vivo biocompatibility with predictable degradation rate.
29525100	6	62	theme	prepared	1247:1254	arg1	scaffold					1256:1263	the prepared scaffold	1243:1263	the prepared scaffold	1243:1263	Compressive strength of the scaffolds was increased from 0.8 MPa to 2.45 MPa on increase in β-TCP content from 10 wt%-30 wt% in the prepared scaffold.
29525100	5	63	theme	120 ± 18.6 μm	1094:1106	arg1	size					1086:1089	average pore size	1073:1089	average pore size of 120 ± 18.6 μm in it	1073:1112	SEM result revealed that incorporation of β-TCP to the extent of 30 wt% resulted in well-shaped and uniformly distributed interconnected pores of average pore size of 120 ± 18.6 μm in it.
29525100	3	64	theme	slurry	651:656	arg1	separation					633:642	solid-liquid phase separation	614:642	solid-liquid phase separation of the slurry containing gelatin, chitosan, β-tricalcium phosphate in varying proportion and subsequent lyophilisation of the phase separated mixture	614:792	The composite scaffolds were fabricated using solid-liquid phase separation of the slurry containing gelatin, chitosan, β-tricalcium phosphate in varying proportion and subsequent lyophilisation of the phase separated mixture.
29525100	7	65	theme	spreading	1402:1410	arg1	behaviour					1412:1420	better spreading behaviour	1395:1420	better spreading behaviour	1395:1420	Human Umbilical Cord derived mesenchymal stem cells (MSCs) exhibited higher degree of lamellopodia and fillopodia extensions and better spreading behaviour onto GCT30 scaffold.
29525100	6	66	theme	-30 wt	1232:1237	arg1	%					1238:1238	10 wt%-30 wt%	1226:1238	10 wt%-30 wt% in the prepared scaffold	1226:1263	Compressive strength of the scaffolds was increased from 0.8 MPa to 2.45 MPa on increase in β-TCP content from 10 wt%-30 wt% in the prepared scaffold.
29525100	6	67	from	%	1238:1238	arg1	scaffold					1256:1263	the prepared scaffold	1243:1263	the prepared scaffold	1243:1263	Compressive strength of the scaffolds was increased from 0.8 MPa to 2.45 MPa on increase in β-TCP content from 10 wt%-30 wt% in the prepared scaffold.
29525100	6	67	from	%	1238:1238	arg1	increase					1195:1202	increase	1195:1202	increase in β-TCP content from 10 wt%-30 wt% in the prepared scaffold	1195:1263	Compressive strength of the scaffolds was increased from 0.8 MPa to 2.45 MPa on increase in β-TCP content from 10 wt%-30 wt% in the prepared scaffold.
29525100	6	67	from	%	1238:1238	arg1	content					1213:1219	β-TCP content	1207:1219	β-TCP content from 10 wt%-30 wt% in the prepared scaffold	1207:1263	Compressive strength of the scaffolds was increased from 0.8 MPa to 2.45 MPa on increase in β-TCP content from 10 wt%-30 wt% in the prepared scaffold.
29525100	2	68	from	pH 10	477:481	arg1	ratio					459:463	a fixed Ca/P molar ratio	440:463	a fixed Ca/P molar ratio of 1.5:1 at pH 10	440:481	Beta tricalcium phosphate (β-TCP) powder was synthesized in size range between 70-100 nm using aqueous precipitation route at a fixed Ca/P molar ratio of 1.5:1 at pH 10 and after subsequent heat treatment of as precipitated powder at 800 °C for 4 hours.
29525100	5	69	theme	well-shaped	1011:1021	arg1	pores					1064:1068	well-shaped and uniformly distributed interconnected pores	1011:1068	well-shaped and uniformly distributed interconnected pores of average pore size of 120 ± 18.6 μm in it	1011:1112	SEM result revealed that incorporation of β-TCP to the extent of 30 wt% resulted in well-shaped and uniformly distributed interconnected pores of average pore size of 120 ± 18.6 μm in it.
29525100	2	70	theme	powder	538:543	arg1	treatment					509:517	subsequent heat treatment	493:517	subsequent heat treatment of as precipitated powder	493:543	Beta tricalcium phosphate (β-TCP) powder was synthesized in size range between 70-100 nm using aqueous precipitation route at a fixed Ca/P molar ratio of 1.5:1 at pH 10 and after subsequent heat treatment of as precipitated powder at 800 °C for 4 hours.
29525100	8	71	theme	osteoblast	1602:1611	arg1	lineage					1613:1619	osteoblast lineage	1602:1619	osteoblast lineage	1602:1619	MTT assay and immunocytochemistry studies with cultured MSCs revealed that GCT30 scaffolds were more conducive to MSC's proliferation and differentiation into osteoblast lineage.
29525100	4	72	theme	high	828:831	arg1	%					846:846	>80%	843:846	>80%	843:846	The prepared scaffolds exhibited high porosity (>80%) with pore sizes ranging between 78-382 μm as determined using Hg-porosimetry.
29525100	4	72	theme	high	828:831	arg1	porosity					833:840	high porosity	828:840	high porosity (>80%) with pore sizes ranging between 78-382 μm	828:889	The prepared scaffolds exhibited high porosity (>80%) with pore sizes ranging between 78-382 μm as determined using Hg-porosimetry.
29525100	1	73	theme	primary	111:117	arg1	aim					119:121	The primary aim	107:121	The primary aim of this study	107:135	The primary aim of this study was to fabricate gelatin/chitosan/β-TCP (GCT) composite scaffold to improve its compressive mechanical behaviour and in-vivo biocompatibility with predictable degradation rate.
29525100	6	74	theme	Compressive	1115:1125	arg1	strength					1127:1134	Compressive strength	1115:1134	Compressive strength of the scaffolds	1115:1151	Compressive strength of the scaffolds was increased from 0.8 MPa to 2.45 MPa on increase in β-TCP content from 10 wt%-30 wt% in the prepared scaffold.
29525100	6	75	from	increase	1195:1202	arg1	%					1238:1238	10 wt%-30 wt%	1226:1238	10 wt%-30 wt% in the prepared scaffold	1226:1263	Compressive strength of the scaffolds was increased from 0.8 MPa to 2.45 MPa on increase in β-TCP content from 10 wt%-30 wt% in the prepared scaffold.
29525100	6	75	from	increase	1195:1202	arg1	content					1213:1219	β-TCP content	1207:1219	β-TCP content from 10 wt%-30 wt% in the prepared scaffold	1207:1263	Compressive strength of the scaffolds was increased from 0.8 MPa to 2.45 MPa on increase in β-TCP content from 10 wt%-30 wt% in the prepared scaffold.
29525100	2	76	theme	precipitation	417:429	arg1	route					431:435	aqueous precipitation route	409:435	aqueous precipitation route	409:435	Beta tricalcium phosphate (β-TCP) powder was synthesized in size range between 70-100 nm using aqueous precipitation route at a fixed Ca/P molar ratio of 1.5:1 at pH 10 and after subsequent heat treatment of as precipitated powder at 800 °C for 4 hours.
29525100	5	77	theme	30 wt	992:996	arg1	%					997:997	30 wt%	992:997	30 wt%	992:997	SEM result revealed that incorporation of β-TCP to the extent of 30 wt% resulted in well-shaped and uniformly distributed interconnected pores of average pore size of 120 ± 18.6 μm in it.
29525100	7	78	theme	higher	1335:1340	arg1	degree					1342:1347	higher degree	1335:1347	higher degree of lamellopodia and fillopodia extensions	1335:1389	Human Umbilical Cord derived mesenchymal stem cells (MSCs) exhibited higher degree of lamellopodia and fillopodia extensions and better spreading behaviour onto GCT30 scaffold.
29525100	9	79	theme	In	1622:1623	arg1	implantation					1630:1641	In vivo implantation	1622:1641	In vivo implantation of GCT30 scaffold subcutaneously into mice	1622:1684	In vivo implantation of GCT30 scaffold subcutaneously into mice did not indicate any significant inflammatory reaction, but ongoing vascularization.
29525100	2	80	theme	molar	453:457	arg1	ratio					459:463	a fixed Ca/P molar ratio	440:463	a fixed Ca/P molar ratio of 1.5:1 at pH 10	440:481	Beta tricalcium phosphate (β-TCP) powder was synthesized in size range between 70-100 nm using aqueous precipitation route at a fixed Ca/P molar ratio of 1.5:1 at pH 10 and after subsequent heat treatment of as precipitated powder at 800 °C for 4 hours.
29525100	2	81	theme	subsequent	493:502	arg1	treatment					509:517	subsequent heat treatment	493:517	subsequent heat treatment of as precipitated powder	493:543	Beta tricalcium phosphate (β-TCP) powder was synthesized in size range between 70-100 nm using aqueous precipitation route at a fixed Ca/P molar ratio of 1.5:1 at pH 10 and after subsequent heat treatment of as precipitated powder at 800 °C for 4 hours.
29525100	2	82	theme	fixed	442:446	arg1	ratio					459:463	a fixed Ca/P molar ratio	440:463	a fixed Ca/P molar ratio of 1.5:1 at pH 10	440:481	Beta tricalcium phosphate (β-TCP) powder was synthesized in size range between 70-100 nm using aqueous precipitation route at a fixed Ca/P molar ratio of 1.5:1 at pH 10 and after subsequent heat treatment of as precipitated powder at 800 °C for 4 hours.
29525100	5	83	theme	pore	1081:1084	arg1	size					1086:1089	average pore size	1073:1089	average pore size of 120 ± 18.6 μm in it	1073:1112	SEM result revealed that incorporation of β-TCP to the extent of 30 wt% resulted in well-shaped and uniformly distributed interconnected pores of average pore size of 120 ± 18.6 μm in it.
29525100	8	84	theme	assay	1447:1451	arg1	studies					1477:1483	MTT assay and immunocytochemistry studies	1443:1483	MTT assay and immunocytochemistry studies with cultured MSCs	1443:1502	MTT assay and immunocytochemistry studies with cultured MSCs revealed that GCT30 scaffolds were more conducive to MSC's proliferation and differentiation into osteoblast lineage.
29525100	2	85	theme	size	374:377	arg1	range					379:383	size range	374:383	size range between 70-100 nm using aqueous precipitation route at a fixed Ca/P molar ratio of 1.5:1 at pH 10 and after subsequent heat treatment of as precipitated powder at 800 °C for 4 hours	374:565	Beta tricalcium phosphate (β-TCP) powder was synthesized in size range between 70-100 nm using aqueous precipitation route at a fixed Ca/P molar ratio of 1.5:1 at pH 10 and after subsequent heat treatment of as precipitated powder at 800 °C for 4 hours.
29525100	7	86	theme	stem	1307:1310	arg1	cells					1312:1316	Human Umbilical Cord derived mesenchymal stem cells	1266:1316	Human Umbilical Cord derived mesenchymal stem cells (MSCs)	1266:1323	Human Umbilical Cord derived mesenchymal stem cells (MSCs) exhibited higher degree of lamellopodia and fillopodia extensions and better spreading behaviour onto GCT30 scaffold.
29525100	7	86	theme	stem	1307:1310	arg1	MSCs					1319:1322	MSCs	1319:1322	MSCs	1319:1322	Human Umbilical Cord derived mesenchymal stem cells (MSCs) exhibited higher degree of lamellopodia and fillopodia extensions and better spreading behaviour onto GCT30 scaffold.
29525100	3	87	theme	β-tricalcium	688:699	arg1	phosphate					701:709	β-tricalcium phosphate	688:709	β-tricalcium phosphate in varying proportion	688:731	The composite scaffolds were fabricated using solid-liquid phase separation of the slurry containing gelatin, chitosan, β-tricalcium phosphate in varying proportion and subsequent lyophilisation of the phase separated mixture.
29525100	8	88	theme	immunocytochemistry	1457:1475	arg1	studies					1477:1483	MTT assay and immunocytochemistry studies	1443:1483	MTT assay and immunocytochemistry studies with cultured MSCs	1443:1502	MTT assay and immunocytochemistry studies with cultured MSCs revealed that GCT30 scaffolds were more conducive to MSC's proliferation and differentiation into osteoblast lineage.
29525100	1	89	theme	in-vivo	254:260	arg1	biocompatibility					262:277	in-vivo biocompatibility	254:277	in-vivo biocompatibility	254:277	The primary aim of this study was to fabricate gelatin/chitosan/β-TCP (GCT) composite scaffold to improve its compressive mechanical behaviour and in-vivo biocompatibility with predictable degradation rate.
29525100	5	90	theme	interconnected	1049:1062	arg1	pores					1064:1068	well-shaped and uniformly distributed interconnected pores	1011:1068	well-shaped and uniformly distributed interconnected pores of average pore size of 120 ± 18.6 μm in it	1011:1112	SEM result revealed that incorporation of β-TCP to the extent of 30 wt% resulted in well-shaped and uniformly distributed interconnected pores of average pore size of 120 ± 18.6 μm in it.
29525100	7	91	theme	derived	1287:1293	arg1	cells					1312:1316	Human Umbilical Cord derived mesenchymal stem cells	1266:1316	Human Umbilical Cord derived mesenchymal stem cells (MSCs)	1266:1323	Human Umbilical Cord derived mesenchymal stem cells (MSCs) exhibited higher degree of lamellopodia and fillopodia extensions and better spreading behaviour onto GCT30 scaffold.
29525100	7	91	theme	derived	1287:1293	arg1	MSCs					1319:1322	MSCs	1319:1322	MSCs	1319:1322	Human Umbilical Cord derived mesenchymal stem cells (MSCs) exhibited higher degree of lamellopodia and fillopodia extensions and better spreading behaviour onto GCT30 scaffold.
29525100	3	92	contain	containing	658:667	arg2	lyophilisation					748:761	subsequent lyophilisation	737:761	subsequent lyophilisation of the phase separated mixture	737:792	The composite scaffolds were fabricated using solid-liquid phase separation of the slurry containing gelatin, chitosan, β-tricalcium phosphate in varying proportion and subsequent lyophilisation of the phase separated mixture.
29525100	3	92	contain	containing	658:667	arg2	phosphate					701:709	β-tricalcium phosphate	688:709	β-tricalcium phosphate in varying proportion	688:731	The composite scaffolds were fabricated using solid-liquid phase separation of the slurry containing gelatin, chitosan, β-tricalcium phosphate in varying proportion and subsequent lyophilisation of the phase separated mixture.
29525100	3	92	contain	containing	658:667	arg1	slurry					651:656	the slurry	647:656	the slurry containing gelatin, chitosan, β-tricalcium phosphate in varying proportion and subsequent lyophilisation of the phase separated mixture	647:792	The composite scaffolds were fabricated using solid-liquid phase separation of the slurry containing gelatin, chitosan, β-tricalcium phosphate in varying proportion and subsequent lyophilisation of the phase separated mixture.
29525100	3	92	contain	containing	658:667	arg2	gelatin					669:675	gelatin	669:675	gelatin	669:675	The composite scaffolds were fabricated using solid-liquid phase separation of the slurry containing gelatin, chitosan, β-tricalcium phosphate in varying proportion and subsequent lyophilisation of the phase separated mixture.
29525100	3	92	contain	containing	658:667	arg2	chitosan					678:685	chitosan	678:685	chitosan	678:685	The composite scaffolds were fabricated using solid-liquid phase separation of the slurry containing gelatin, chitosan, β-tricalcium phosphate in varying proportion and subsequent lyophilisation of the phase separated mixture.
29525100	3	93	from	phosphate	701:709	arg1	proportion					722:731	varying proportion	714:731	varying proportion	714:731	The composite scaffolds were fabricated using solid-liquid phase separation of the slurry containing gelatin, chitosan, β-tricalcium phosphate in varying proportion and subsequent lyophilisation of the phase separated mixture.
29525100	3	94	theme	subsequent	737:746	arg1	lyophilisation					748:761	subsequent lyophilisation	737:761	subsequent lyophilisation of the phase separated mixture	737:792	The composite scaffolds were fabricated using solid-liquid phase separation of the slurry containing gelatin, chitosan, β-tricalcium phosphate in varying proportion and subsequent lyophilisation of the phase separated mixture.
29525100	7	95	theme	Umbilical	1272:1280	arg1	cells					1312:1316	Human Umbilical Cord derived mesenchymal stem cells	1266:1316	Human Umbilical Cord derived mesenchymal stem cells (MSCs)	1266:1323	Human Umbilical Cord derived mesenchymal stem cells (MSCs) exhibited higher degree of lamellopodia and fillopodia extensions and better spreading behaviour onto GCT30 scaffold.
29525100	7	95	theme	Umbilical	1272:1280	arg1	MSCs					1319:1322	MSCs	1319:1322	MSCs	1319:1322	Human Umbilical Cord derived mesenchymal stem cells (MSCs) exhibited higher degree of lamellopodia and fillopodia extensions and better spreading behaviour onto GCT30 scaffold.
30669264	4	0	theme	experimental	670:681	arg1	design					683:688	a Box⁻Behnken experimental design	656:688	a Box⁻Behnken experimental design	656:688	Twenty experiments were carried out and a Box⁻Behnken experimental design was used to evaluate the significance of these independent variables related to encapsulation efficiency (EE).
30669264	6	1	theme	NPs	943:945	arg1	Sizes					899:903	Sizes	899:903	Sizes	899:903	Sizes and polydispersities of the optimized NPs were studied by dynamic light scattering (DLS).
30669264	6	1	theme	NPs	943:945	arg1	polydispersities					909:924	polydispersities	909:924	polydispersities	909:924	Sizes and polydispersities of the optimized NPs were studied by dynamic light scattering (DLS).
30669264	9	2	theme	colon	1352:1356	arg1	conditions					1358:1367	simulated colon conditions	1342:1367	simulated colon conditions	1342:1367	Release patterns in simulated colon conditions for 48 h displayed significant differences between the NPs (final cumulative drug release: 79⁻81%), and the CTS-NPs composites (29⁻34%).
30669264	2	3	theme	drug	472:475	arg1	depots					477:482	long-lasting mucoadhesive drug depots	446:482	long-lasting mucoadhesive drug depots	446:482	Such systems are endowed with potential therapeutic effects on inflammatory bowel diseases (IBD), such as Crohn's disease (CD) and ulcerative colitis, through the sustained colonic release of RES from long-lasting mucoadhesive drug depots.
30669264	3	4	theme	RES	500:502	arg1	loading					489:495	The loading	485:495	The loading of RES into nanoparticles (NPs)	485:527	The loading of RES into nanoparticles (NPs) was optimized regarding two independent variables: RES/polymer ratio, and temperature.
30669264	2	5	theme	inflammatory	308:319	arg1	diseases					327:334	inflammatory bowel diseases	308:334	inflammatory bowel diseases (IBD)	308:340	Such systems are endowed with potential therapeutic effects on inflammatory bowel diseases (IBD), such as Crohn's disease (CD) and ulcerative colitis, through the sustained colonic release of RES from long-lasting mucoadhesive drug depots.
30669264	2	5	theme	inflammatory	308:319	arg1	disease					359:365	Crohn's disease	351:365	Crohn's disease (CD)	351:370	Such systems are endowed with potential therapeutic effects on inflammatory bowel diseases (IBD), such as Crohn's disease (CD) and ulcerative colitis, through the sustained colonic release of RES from long-lasting mucoadhesive drug depots.
30669264	2	5	theme	inflammatory	308:319	arg1	colitis					387:393	ulcerative colitis	376:393	ulcerative colitis	376:393	Such systems are endowed with potential therapeutic effects on inflammatory bowel diseases (IBD), such as Crohn's disease (CD) and ulcerative colitis, through the sustained colonic release of RES from long-lasting mucoadhesive drug depots.
30669264	2	5	theme	inflammatory	308:319	arg1	IBD					337:339	IBD	337:339	IBD	337:339	Such systems are endowed with potential therapeutic effects on inflammatory bowel diseases (IBD), such as Crohn's disease (CD) and ulcerative colitis, through the sustained colonic release of RES from long-lasting mucoadhesive drug depots.
30669264	9	6	dep	release	1451:1457	arg1	%					1465:1465	79⁻81%	1460:1465	final cumulative drug release: 79⁻81%	1429:1465	Release patterns in simulated colon conditions for 48 h displayed significant differences between the NPs (final cumulative drug release: 79⁻81%), and the CTS-NPs composites (29⁻34%).
30669264	4	7	used	used	694:697	arg2	design					683:688	a Box⁻Behnken experimental design	656:688	a Box⁻Behnken experimental design	656:688	Twenty experiments were carried out and a Box⁻Behnken experimental design was used to evaluate the significance of these independent variables related to encapsulation efficiency (EE).
30669264	4	8	theme	variables	749:757	arg1	significance					715:726	the significance	711:726	the significance of these independent variables related to encapsulation efficiency (EE)	711:798	Twenty experiments were carried out and a Box⁻Behnken experimental design was used to evaluate the significance of these independent variables related to encapsulation efficiency (EE).
30669264	5	9	theme	0.75:1	887:892	arg1	ratio					878:882	RES/polymer ratio	866:882	RES/polymer ratio of 0.75:1 w/w	866:896	The enhanced RES EE values were achieved in 24 h at 39 °C and at RES/polymer ratio of 0.75:1 w/w.
30669264	8	10	theme	Macro-	1128:1133	arg1	features					1151:1158	Macro- and microscopic features	1128:1158	Macro- and microscopic features (morphology and porosity studied by SEM and spreadability)	1128:1217	Macro- and microscopic features (morphology and porosity studied by SEM and spreadability), thermal stability (studied by TGA), and release kinetics of the RES-loaded CTS-NPs were investigated.
30669264	8	10	theme	Macro-	1128:1133	arg1	porosity					1176:1183	porosity	1176:1183	porosity	1176:1183	Macro- and microscopic features (morphology and porosity studied by SEM and spreadability), thermal stability (studied by TGA), and release kinetics of the RES-loaded CTS-NPs were investigated.
30669264	8	10	theme	Macro-	1128:1133	arg1	morphology					1161:1170	morphology	1161:1170	morphology	1161:1170	Macro- and microscopic features (morphology and porosity studied by SEM and spreadability), thermal stability (studied by TGA), and release kinetics of the RES-loaded CTS-NPs were investigated.
30669264	9	11	theme	final	1429:1433	arg1	release					1451:1457	final cumulative drug release	1429:1457	final cumulative drug release: 79⁻81%	1429:1465	Release patterns in simulated colon conditions for 48 h displayed significant differences between the NPs (final cumulative drug release: 79⁻81%), and the CTS-NPs composites (29⁻34%).
30669264	7	12	theme	RES-loaded	1037:1046	arg1	NPs					1048:1050	the RES-loaded NPs	1033:1050	the RES-loaded NPs	1033:1050	Chitosan (CTS) dispersions containing the RES-loaded NPs were ionically gelled with tricarballylic acid to yield CTS-NPs composites.
30669264	4	13	theme	encapsulation	770:782	arg1	EE					796:797	EE	796:797	EE	796:797	Twenty experiments were carried out and a Box⁻Behnken experimental design was used to evaluate the significance of these independent variables related to encapsulation efficiency (EE).
30669264	4	13	theme	encapsulation	770:782	arg1	efficiency					784:793	encapsulation efficiency	770:793	encapsulation efficiency (EE)	770:798	Twenty experiments were carried out and a Box⁻Behnken experimental design was used to evaluate the significance of these independent variables related to encapsulation efficiency (EE).
30669264	2	14	dep	potential	275:283	arg1	therapeutic					285:295	therapeutic	285:295	therapeutic	285:295	Such systems are endowed with potential therapeutic effects on inflammatory bowel diseases (IBD), such as Crohn's disease (CD) and ulcerative colitis, through the sustained colonic release of RES from long-lasting mucoadhesive drug depots.
30669264	2	15	theme	ulcerative	376:385	arg1	colitis					387:393	ulcerative colitis	376:393	ulcerative colitis	376:393	Such systems are endowed with potential therapeutic effects on inflammatory bowel diseases (IBD), such as Crohn's disease (CD) and ulcerative colitis, through the sustained colonic release of RES from long-lasting mucoadhesive drug depots.
30669264	0	16	theme	Colon-Specific	61:74	arg1	Release					88:94	Sustained and Colon-Specific Resveratrol Release	47:94	Sustained and Colon-Specific Resveratrol Release	47:94	Nanostructured Chitosan-Based Biomaterials for Sustained and Colon-Specific Resveratrol Release.
30669264	9	17	theme	CTS-NPs	1477:1483	arg1	composites					1485:1494	the CTS-NPs composites	1473:1494	the CTS-NPs composites (29⁻34%)	1473:1503	Release patterns in simulated colon conditions for 48 h displayed significant differences between the NPs (final cumulative drug release: 79⁻81%), and the CTS-NPs composites (29⁻34%).
30669264	9	17	theme	CTS-NPs	1477:1483	arg1	%					1502:1502	29⁻34%	1497:1502	29⁻34%	1497:1502	Release patterns in simulated colon conditions for 48 h displayed significant differences between the NPs (final cumulative drug release: 79⁻81%), and the CTS-NPs composites (29⁻34%).
30669264	3	18	dep	variables	569:577	arg1	variables					569:577	two independent variables	553:577	two independent variables: RES/polymer ratio, and temperature	553:613	The loading of RES into nanoparticles (NPs) was optimized regarding two independent variables: RES/polymer ratio, and temperature.
30669264	3	18	dep	variables	569:577	arg1	ratio					592:596	RES/polymer ratio	580:596	RES/polymer ratio	580:596	The loading of RES into nanoparticles (NPs) was optimized regarding two independent variables: RES/polymer ratio, and temperature.
30669264	3	18	dep	variables	569:577	arg1	temperature					603:613	temperature	603:613	temperature	603:613	The loading of RES into nanoparticles (NPs) was optimized regarding two independent variables: RES/polymer ratio, and temperature.
30669264	0	19	theme	Chitosan-Based	15:28	arg1	Biomaterials					30:41	Nanostructured Chitosan-Based Biomaterials	0:41	Nanostructured Chitosan-Based Biomaterials for Sustained and Colon-Specific Resveratrol Release.	0:95	Nanostructured Chitosan-Based Biomaterials for Sustained and Colon-Specific Resveratrol Release.
30669264	1	20	theme	present	104:110	arg1	work					112:115	the present work	100:115	the present work	100:115	In the present work, we demonstrate the preparation of chitosan-based composites as vehicles of the natural occurring multi-drug resveratrol (RES).
30669264	8	21	dep	features	1151:1158	arg1	features					1151:1158	Macro- and microscopic features	1128:1158	Macro- and microscopic features (morphology and porosity studied by SEM and spreadability)	1128:1217	Macro- and microscopic features (morphology and porosity studied by SEM and spreadability), thermal stability (studied by TGA), and release kinetics of the RES-loaded CTS-NPs were investigated.
30669264	8	21	dep	features	1151:1158	arg1	porosity					1176:1183	porosity	1176:1183	porosity	1176:1183	Macro- and microscopic features (morphology and porosity studied by SEM and spreadability), thermal stability (studied by TGA), and release kinetics of the RES-loaded CTS-NPs were investigated.
30669264	8	21	dep	features	1151:1158	arg1	morphology					1161:1170	morphology	1161:1170	morphology	1161:1170	Macro- and microscopic features (morphology and porosity studied by SEM and spreadability), thermal stability (studied by TGA), and release kinetics of the RES-loaded CTS-NPs were investigated.
30669264	1	22	theme	natural	197:203	arg1	resveratrol					226:236	the natural occurring multi-drug resveratrol	193:236	the natural occurring multi-drug resveratrol (RES)	193:242	In the present work, we demonstrate the preparation of chitosan-based composites as vehicles of the natural occurring multi-drug resveratrol (RES).
30669264	1	22	theme	natural	197:203	arg1	RES					239:241	RES	239:241	RES	239:241	In the present work, we demonstrate the preparation of chitosan-based composites as vehicles of the natural occurring multi-drug resveratrol (RES).
30669264	0	23	theme	Nanostructured	0:13	arg1	Biomaterials					30:41	Nanostructured Chitosan-Based Biomaterials	0:41	Nanostructured Chitosan-Based Biomaterials for Sustained and Colon-Specific Resveratrol Release.	0:95	Nanostructured Chitosan-Based Biomaterials for Sustained and Colon-Specific Resveratrol Release.
30669264	6	24	theme	optimized	933:941	arg1	NPs					943:945	the optimized NPs	929:945	the optimized NPs	929:945	Sizes and polydispersities of the optimized NPs were studied by dynamic light scattering (DLS).
30669264	8	25	theme	CTS-NPs	1295:1301	arg1	features					1151:1158	Macro- and microscopic features	1128:1158	Macro- and microscopic features (morphology and porosity studied by SEM and spreadability)	1128:1217	Macro- and microscopic features (morphology and porosity studied by SEM and spreadability), thermal stability (studied by TGA), and release kinetics of the RES-loaded CTS-NPs were investigated.
30669264	8	25	theme	CTS-NPs	1295:1301	arg1	kinetics					1268:1275	release kinetics	1260:1275	release kinetics of the RES-loaded CTS-NPs	1260:1301	Macro- and microscopic features (morphology and porosity studied by SEM and spreadability), thermal stability (studied by TGA), and release kinetics of the RES-loaded CTS-NPs were investigated.
30669264	8	25	theme	CTS-NPs	1295:1301	arg1	porosity					1176:1183	porosity	1176:1183	porosity	1176:1183	Macro- and microscopic features (morphology and porosity studied by SEM and spreadability), thermal stability (studied by TGA), and release kinetics of the RES-loaded CTS-NPs were investigated.
30669264	8	25	theme	CTS-NPs	1295:1301	arg1	morphology					1161:1170	morphology	1161:1170	morphology	1161:1170	Macro- and microscopic features (morphology and porosity studied by SEM and spreadability), thermal stability (studied by TGA), and release kinetics of the RES-loaded CTS-NPs were investigated.
30669264	8	25	theme	CTS-NPs	1295:1301	arg1	stability					1228:1236	thermal stability	1220:1236	thermal stability (studied by TGA)	1220:1253	Macro- and microscopic features (morphology and porosity studied by SEM and spreadability), thermal stability (studied by TGA), and release kinetics of the RES-loaded CTS-NPs were investigated.
30669264	1	26	theme	occurring	205:213	arg1	resveratrol					226:236	the natural occurring multi-drug resveratrol	193:236	the natural occurring multi-drug resveratrol (RES)	193:242	In the present work, we demonstrate the preparation of chitosan-based composites as vehicles of the natural occurring multi-drug resveratrol (RES).
30669264	1	26	theme	occurring	205:213	arg1	RES					239:241	RES	239:241	RES	239:241	In the present work, we demonstrate the preparation of chitosan-based composites as vehicles of the natural occurring multi-drug resveratrol (RES).
30669264	2	27	theme	colonic	418:424	arg1	release					426:432	the sustained colonic release	404:432	the sustained colonic release of RES from long-lasting mucoadhesive drug depots	404:482	Such systems are endowed with potential therapeutic effects on inflammatory bowel diseases (IBD), such as Crohn's disease (CD) and ulcerative colitis, through the sustained colonic release of RES from long-lasting mucoadhesive drug depots.
30669264	4	28	theme	independent	737:747	arg1	variables					749:757	these independent variables	731:757	these independent variables related to encapsulation efficiency (EE)	731:798	Twenty experiments were carried out and a Box⁻Behnken experimental design was used to evaluate the significance of these independent variables related to encapsulation efficiency (EE).
30669264	1	29	theme	multi-drug	215:224	arg1	resveratrol					226:236	the natural occurring multi-drug resveratrol	193:236	the natural occurring multi-drug resveratrol (RES)	193:242	In the present work, we demonstrate the preparation of chitosan-based composites as vehicles of the natural occurring multi-drug resveratrol (RES).
30669264	1	29	theme	multi-drug	215:224	arg1	RES					239:241	RES	239:241	RES	239:241	In the present work, we demonstrate the preparation of chitosan-based composites as vehicles of the natural occurring multi-drug resveratrol (RES).
30669264	2	30	theme	sustained	408:416	arg1	release					426:432	the sustained colonic release	404:432	the sustained colonic release of RES from long-lasting mucoadhesive drug depots	404:482	Such systems are endowed with potential therapeutic effects on inflammatory bowel diseases (IBD), such as Crohn's disease (CD) and ulcerative colitis, through the sustained colonic release of RES from long-lasting mucoadhesive drug depots.
30669264	8	31	theme	RES-loaded	1284:1293	arg1	CTS-NPs					1295:1301	the RES-loaded CTS-NPs	1280:1301	the RES-loaded CTS-NPs	1280:1301	Macro- and microscopic features (morphology and porosity studied by SEM and spreadability), thermal stability (studied by TGA), and release kinetics of the RES-loaded CTS-NPs were investigated.
30669264	2	32	from	depots	477:482	arg1	release					426:432	the sustained colonic release	404:432	the sustained colonic release of RES from long-lasting mucoadhesive drug depots	404:482	Such systems are endowed with potential therapeutic effects on inflammatory bowel diseases (IBD), such as Crohn's disease (CD) and ulcerative colitis, through the sustained colonic release of RES from long-lasting mucoadhesive drug depots.
30669264	2	33	theme	bowel	321:325	arg1	diseases					327:334	inflammatory bowel diseases	308:334	inflammatory bowel diseases (IBD)	308:340	Such systems are endowed with potential therapeutic effects on inflammatory bowel diseases (IBD), such as Crohn's disease (CD) and ulcerative colitis, through the sustained colonic release of RES from long-lasting mucoadhesive drug depots.
30669264	2	33	theme	bowel	321:325	arg1	disease					359:365	Crohn's disease	351:365	Crohn's disease (CD)	351:370	Such systems are endowed with potential therapeutic effects on inflammatory bowel diseases (IBD), such as Crohn's disease (CD) and ulcerative colitis, through the sustained colonic release of RES from long-lasting mucoadhesive drug depots.
30669264	2	33	theme	bowel	321:325	arg1	colitis					387:393	ulcerative colitis	376:393	ulcerative colitis	376:393	Such systems are endowed with potential therapeutic effects on inflammatory bowel diseases (IBD), such as Crohn's disease (CD) and ulcerative colitis, through the sustained colonic release of RES from long-lasting mucoadhesive drug depots.
30669264	2	33	theme	bowel	321:325	arg1	IBD					337:339	IBD	337:339	IBD	337:339	Such systems are endowed with potential therapeutic effects on inflammatory bowel diseases (IBD), such as Crohn's disease (CD) and ulcerative colitis, through the sustained colonic release of RES from long-lasting mucoadhesive drug depots.
30669264	1	34	theme	resveratrol	226:236	arg1	vehicles					181:188	vehicles	181:188	vehicles of the natural occurring multi-drug resveratrol (RES)	181:242	In the present work, we demonstrate the preparation of chitosan-based composites as vehicles of the natural occurring multi-drug resveratrol (RES).
30669264	2	35	theme	mucoadhesive	459:470	arg1	depots					477:482	long-lasting mucoadhesive drug depots	446:482	long-lasting mucoadhesive drug depots	446:482	Such systems are endowed with potential therapeutic effects on inflammatory bowel diseases (IBD), such as Crohn's disease (CD) and ulcerative colitis, through the sustained colonic release of RES from long-lasting mucoadhesive drug depots.
30669264	8	36	theme	microscopic	1139:1149	arg1	features					1151:1158	Macro- and microscopic features	1128:1158	Macro- and microscopic features (morphology and porosity studied by SEM and spreadability)	1128:1217	Macro- and microscopic features (morphology and porosity studied by SEM and spreadability), thermal stability (studied by TGA), and release kinetics of the RES-loaded CTS-NPs were investigated.
30669264	8	36	theme	microscopic	1139:1149	arg1	porosity					1176:1183	porosity	1176:1183	porosity	1176:1183	Macro- and microscopic features (morphology and porosity studied by SEM and spreadability), thermal stability (studied by TGA), and release kinetics of the RES-loaded CTS-NPs were investigated.
30669264	8	36	theme	microscopic	1139:1149	arg1	morphology					1161:1170	morphology	1161:1170	morphology	1161:1170	Macro- and microscopic features (morphology and porosity studied by SEM and spreadability), thermal stability (studied by TGA), and release kinetics of the RES-loaded CTS-NPs were investigated.
30669264	0	37	theme	Sustained	47:55	arg1	Release					88:94	Sustained and Colon-Specific Resveratrol Release	47:94	Sustained and Colon-Specific Resveratrol Release	47:94	Nanostructured Chitosan-Based Biomaterials for Sustained and Colon-Specific Resveratrol Release.
30669264	2	38	with	endowed	262:268	arg1	effects					297:303	potential therapeutic effects	275:303	potential therapeutic effects on inflammatory bowel diseases (IBD), such as Crohn's disease (CD) and ulcerative colitis	275:393	Such systems are endowed with potential therapeutic effects on inflammatory bowel diseases (IBD), such as Crohn's disease (CD) and ulcerative colitis, through the sustained colonic release of RES from long-lasting mucoadhesive drug depots.
30669264	2	39	theme	long-lasting	446:457	arg1	depots					477:482	long-lasting mucoadhesive drug depots	446:482	long-lasting mucoadhesive drug depots	446:482	Such systems are endowed with potential therapeutic effects on inflammatory bowel diseases (IBD), such as Crohn's disease (CD) and ulcerative colitis, through the sustained colonic release of RES from long-lasting mucoadhesive drug depots.
30669264	2	40	theme	Such	245:248	arg1	systems					250:256	Such systems	245:256	Such systems	245:256	Such systems are endowed with potential therapeutic effects on inflammatory bowel diseases (IBD), such as Crohn's disease (CD) and ulcerative colitis, through the sustained colonic release of RES from long-lasting mucoadhesive drug depots.
30669264	5	41	theme	enhanced	805:812	arg1	values					821:826	The enhanced RES EE values	801:826	The enhanced RES EE values	801:826	The enhanced RES EE values were achieved in 24 h at 39 °C and at RES/polymer ratio of 0.75:1 w/w.
30669264	0	42	theme	Resveratrol	76:86	arg1	Release					88:94	Sustained and Colon-Specific Resveratrol Release	47:94	Sustained and Colon-Specific Resveratrol Release	47:94	Nanostructured Chitosan-Based Biomaterials for Sustained and Colon-Specific Resveratrol Release.
30669264	3	43	theme	independent	557:567	arg1	variables					569:577	two independent variables	553:577	two independent variables: RES/polymer ratio, and temperature	553:613	The loading of RES into nanoparticles (NPs) was optimized regarding two independent variables: RES/polymer ratio, and temperature.
30669264	3	43	theme	independent	557:567	arg1	ratio					592:596	RES/polymer ratio	580:596	RES/polymer ratio	580:596	The loading of RES into nanoparticles (NPs) was optimized regarding two independent variables: RES/polymer ratio, and temperature.
30669264	3	43	theme	independent	557:567	arg1	temperature					603:613	temperature	603:613	temperature	603:613	The loading of RES into nanoparticles (NPs) was optimized regarding two independent variables: RES/polymer ratio, and temperature.
30669264	5	44	theme	RES/polymer	866:876	arg1	ratio					878:882	RES/polymer ratio	866:882	RES/polymer ratio of 0.75:1 w/w	866:896	The enhanced RES EE values were achieved in 24 h at 39 °C and at RES/polymer ratio of 0.75:1 w/w.
30669264	2	45	theme	RES	437:439	arg1	release					426:432	the sustained colonic release	404:432	the sustained colonic release of RES from long-lasting mucoadhesive drug depots	404:482	Such systems are endowed with potential therapeutic effects on inflammatory bowel diseases (IBD), such as Crohn's disease (CD) and ulcerative colitis, through the sustained colonic release of RES from long-lasting mucoadhesive drug depots.
30669264	7	46	theme	Chitosan	995:1002	arg1	dispersions					1010:1020	Chitosan (CTS) dispersions	995:1020	Chitosan (CTS) dispersions containing the RES-loaded NPs	995:1050	Chitosan (CTS) dispersions containing the RES-loaded NPs were ionically gelled with tricarballylic acid to yield CTS-NPs composites.
30669264	9	47	theme	cumulative	1435:1444	arg1	release					1451:1457	final cumulative drug release	1429:1457	final cumulative drug release: 79⁻81%	1429:1465	Release patterns in simulated colon conditions for 48 h displayed significant differences between the NPs (final cumulative drug release: 79⁻81%), and the CTS-NPs composites (29⁻34%).
30669264	2	48	from	effects	297:303	arg1	diseases					327:334	inflammatory bowel diseases	308:334	inflammatory bowel diseases (IBD)	308:340	Such systems are endowed with potential therapeutic effects on inflammatory bowel diseases (IBD), such as Crohn's disease (CD) and ulcerative colitis, through the sustained colonic release of RES from long-lasting mucoadhesive drug depots.
30669264	2	48	from	effects	297:303	arg1	disease					359:365	Crohn's disease	351:365	Crohn's disease (CD)	351:370	Such systems are endowed with potential therapeutic effects on inflammatory bowel diseases (IBD), such as Crohn's disease (CD) and ulcerative colitis, through the sustained colonic release of RES from long-lasting mucoadhesive drug depots.
30669264	2	48	from	effects	297:303	arg1	colitis					387:393	ulcerative colitis	376:393	ulcerative colitis	376:393	Such systems are endowed with potential therapeutic effects on inflammatory bowel diseases (IBD), such as Crohn's disease (CD) and ulcerative colitis, through the sustained colonic release of RES from long-lasting mucoadhesive drug depots.
30669264	2	48	from	effects	297:303	arg1	IBD					337:339	IBD	337:339	IBD	337:339	Such systems are endowed with potential therapeutic effects on inflammatory bowel diseases (IBD), such as Crohn's disease (CD) and ulcerative colitis, through the sustained colonic release of RES from long-lasting mucoadhesive drug depots.
30669264	7	49	theme	CTS-NPs	1108:1114	arg1	composites					1116:1125	CTS-NPs composites	1108:1125	CTS-NPs composites	1108:1125	Chitosan (CTS) dispersions containing the RES-loaded NPs were ionically gelled with tricarballylic acid to yield CTS-NPs composites.
30669264	9	50	dep	NPs	1424:1426	arg1	release					1451:1457	final cumulative drug release	1429:1457	final cumulative drug release: 79⁻81%	1429:1465	Release patterns in simulated colon conditions for 48 h displayed significant differences between the NPs (final cumulative drug release: 79⁻81%), and the CTS-NPs composites (29⁻34%).
30669264	2	51	theme	potential	275:283	arg1	effects					297:303	potential therapeutic effects	275:303	potential therapeutic effects on inflammatory bowel diseases (IBD), such as Crohn's disease (CD) and ulcerative colitis	275:393	Such systems are endowed with potential therapeutic effects on inflammatory bowel diseases (IBD), such as Crohn's disease (CD) and ulcerative colitis, through the sustained colonic release of RES from long-lasting mucoadhesive drug depots.
30669264	3	52	theme	RES/polymer	580:590	arg1	variables					569:577	two independent variables	553:577	two independent variables: RES/polymer ratio, and temperature	553:613	The loading of RES into nanoparticles (NPs) was optimized regarding two independent variables: RES/polymer ratio, and temperature.
30669264	3	52	theme	RES/polymer	580:590	arg1	ratio					592:596	RES/polymer ratio	580:596	RES/polymer ratio	580:596	The loading of RES into nanoparticles (NPs) was optimized regarding two independent variables: RES/polymer ratio, and temperature.
30669264	9	53	theme	drug	1446:1449	arg1	release					1451:1457	final cumulative drug release	1429:1457	final cumulative drug release: 79⁻81%	1429:1465	Release patterns in simulated colon conditions for 48 h displayed significant differences between the NPs (final cumulative drug release: 79⁻81%), and the CTS-NPs composites (29⁻34%).
30669264	7	54	contain	containing	1022:1031	arg2	NPs					1048:1050	the RES-loaded NPs	1033:1050	the RES-loaded NPs	1033:1050	Chitosan (CTS) dispersions containing the RES-loaded NPs were ionically gelled with tricarballylic acid to yield CTS-NPs composites.
30669264	7	54	contain	containing	1022:1031	arg1	dispersions					1010:1020	Chitosan (CTS) dispersions	995:1020	Chitosan (CTS) dispersions containing the RES-loaded NPs	995:1050	Chitosan (CTS) dispersions containing the RES-loaded NPs were ionically gelled with tricarballylic acid to yield CTS-NPs composites.
30669264	9	55	theme	Release	1322:1328	arg1	patterns					1330:1337	Release patterns	1322:1337	Release patterns in simulated colon conditions for 48 h	1322:1376	Release patterns in simulated colon conditions for 48 h displayed significant differences between the NPs (final cumulative drug release: 79⁻81%), and the CTS-NPs composites (29⁻34%).
30669264	9	56	from	patterns	1330:1337	arg1	conditions					1358:1367	simulated colon conditions	1342:1367	simulated colon conditions	1342:1367	Release patterns in simulated colon conditions for 48 h displayed significant differences between the NPs (final cumulative drug release: 79⁻81%), and the CTS-NPs composites (29⁻34%).
30669264	4	57	theme	related	759:765	arg1	variables					749:757	these independent variables	731:757	these independent variables related to encapsulation efficiency (EE)	731:798	Twenty experiments were carried out and a Box⁻Behnken experimental design was used to evaluate the significance of these independent variables related to encapsulation efficiency (EE).
30669264	8	58	dep	stability	1228:1236	arg1	studied					1239:1245	studied	1239:1245	studied by TGA	1239:1252	Macro- and microscopic features (morphology and porosity studied by SEM and spreadability), thermal stability (studied by TGA), and release kinetics of the RES-loaded CTS-NPs were investigated.
30669264	4	59	theme	Box⁻Behnken	658:668	arg1	design					683:688	a Box⁻Behnken experimental design	656:688	a Box⁻Behnken experimental design	656:688	Twenty experiments were carried out and a Box⁻Behnken experimental design was used to evaluate the significance of these independent variables related to encapsulation efficiency (EE).
30669264	8	60	theme	release	1260:1266	arg1	kinetics					1268:1275	release kinetics	1260:1275	release kinetics of the RES-loaded CTS-NPs	1260:1301	Macro- and microscopic features (morphology and porosity studied by SEM and spreadability), thermal stability (studied by TGA), and release kinetics of the RES-loaded CTS-NPs were investigated.
30669264	8	61	theme	thermal	1220:1226	arg1	stability					1228:1236	thermal stability	1220:1236	thermal stability (studied by TGA)	1220:1253	Macro- and microscopic features (morphology and porosity studied by SEM and spreadability), thermal stability (studied by TGA), and release kinetics of the RES-loaded CTS-NPs were investigated.
30669264	5	62	theme	EE	818:819	arg1	values					821:826	The enhanced RES EE values	801:826	The enhanced RES EE values	801:826	The enhanced RES EE values were achieved in 24 h at 39 °C and at RES/polymer ratio of 0.75:1 w/w.
30669264	1	63	theme	chitosan-based	152:165	arg1	composites					167:176	chitosan-based composites	152:176	chitosan-based composites as vehicles of the natural occurring multi-drug resveratrol (RES)	152:242	In the present work, we demonstrate the preparation of chitosan-based composites as vehicles of the natural occurring multi-drug resveratrol (RES).
30669264	6	64	theme	light	971:975	arg1	DLS					989:991	DLS	989:991	DLS	989:991	Sizes and polydispersities of the optimized NPs were studied by dynamic light scattering (DLS).
30669264	6	64	theme	light	971:975	arg1	scattering					977:986	dynamic light scattering	963:986	dynamic light scattering (DLS)	963:992	Sizes and polydispersities of the optimized NPs were studied by dynamic light scattering (DLS).
30669264	7	65	theme	tricarballylic	1079:1092	arg1	acid					1094:1097	tricarballylic acid	1079:1097	tricarballylic acid	1079:1097	Chitosan (CTS) dispersions containing the RES-loaded NPs were ionically gelled with tricarballylic acid to yield CTS-NPs composites.
30669264	9	66	theme	significant	1388:1398	arg1	differences					1400:1410	significant differences	1388:1410	significant differences between the NPs (final cumulative drug release: 79⁻81%), and the CTS-NPs composites (29⁻34%)	1388:1503	Release patterns in simulated colon conditions for 48 h displayed significant differences between the NPs (final cumulative drug release: 79⁻81%), and the CTS-NPs composites (29⁻34%).
30669264	5	67	theme	RES	814:816	arg1	values					821:826	The enhanced RES EE values	801:826	The enhanced RES EE values	801:826	The enhanced RES EE values were achieved in 24 h at 39 °C and at RES/polymer ratio of 0.75:1 w/w.
30669264	1	68	theme	composites	167:176	arg1	preparation					137:147	the preparation	133:147	the preparation of chitosan-based composites as vehicles of the natural occurring multi-drug resveratrol (RES)	133:242	In the present work, we demonstrate the preparation of chitosan-based composites as vehicles of the natural occurring multi-drug resveratrol (RES).
30669264	9	69	theme	simulated	1342:1350	arg1	conditions					1358:1367	simulated colon conditions	1342:1367	simulated colon conditions	1342:1367	Release patterns in simulated colon conditions for 48 h displayed significant differences between the NPs (final cumulative drug release: 79⁻81%), and the CTS-NPs composites (29⁻34%).
30669264	6	70	theme	dynamic	963:969	arg1	DLS					989:991	DLS	989:991	DLS	989:991	Sizes and polydispersities of the optimized NPs were studied by dynamic light scattering (DLS).
30669264	6	70	theme	dynamic	963:969	arg1	scattering					977:986	dynamic light scattering	963:986	dynamic light scattering (DLS)	963:992	Sizes and polydispersities of the optimized NPs were studied by dynamic light scattering (DLS).
31301831	9	0	theme	sampling	1304:1311	arg1	days					1313:1316	the blood sampling days	1294:1316	the blood sampling days	1294:1316	Milk samples for analysis of fat, protein, lactose, and FA concentrations were taken on the blood sampling days.
31301831	17	1	theme	advanced	2375:2382	arg1	model					2384:2388	The most advanced model	2366:2388	The most advanced model using both milk and body traits	2366:2420	The most advanced model using both milk and body traits gave a slightly better fit than the model with only milk traits (R2cv = 0.63; RMSE = 0.176 mmol/L).
31301831	6	2	theme	second	1018:1023	arg1	lactation					1025:1033	a second lactation	1016:1033	a second lactation	1016:1033	Data were collected from 2 research herds during 2013 to 2016 and included 137 Nordic Red dairy cows, all of which had a first lactation and 59 of which also had a second lactation.
31301831	14	3	theme	cross-validation	1922:1937	arg1	coefficient					1939:1949	a cross-validation coefficient	1920:1949	a cross-validation coefficient of determination (R2cv) of 0.51	1920:1981	The model resulted in a cross-validation coefficient of determination (R2cv) of 0.51 and a root mean squared error (RMSE) of 0.196 mmol/L.
31301831	6	4	theme	first	975:979	arg1	lactation					981:989	a first lactation	973:989	a first lactation	973:989	Data were collected from 2 research herds during 2013 to 2016 and included 137 Nordic Red dairy cows, all of which had a first lactation and 59 of which also had a second lactation.
31301831	3	5	from	fat	512:514	arg1	ΔBCS					500:503	ΔBCS	500:503	ΔBCS	500:503	Cows' ES can be estimated by calculating the energy balance from energy intake and output and predicted by indicator traits such as change in body weight (ΔBW), change in body condition score (ΔBCS), milk fat:protein ratio (FPR), or milk fatty acid (FA) composition.
31301831	3	5	from	fat	512:514	arg1	score					493:497	body condition score	478:497	body condition score (ΔBCS)	478:504	Cows' ES can be estimated by calculating the energy balance from energy intake and output and predicted by indicator traits such as change in body weight (ΔBW), change in body condition score (ΔBCS), milk fat:protein ratio (FPR), or milk fatty acid (FA) composition.
31301831	3	6	theme	FA	557:558	arg1	composition					561:571	milk fatty acid (FA) composition	540:571	milk fatty acid (FA) composition	540:571	Cows' ES can be estimated by calculating the energy balance from energy intake and output and predicted by indicator traits such as change in body weight (ΔBW), change in body condition score (ΔBCS), milk fat:protein ratio (FPR), or milk fatty acid (FA) composition.
31301831	15	7	theme	=	2200:2200	arg1	R2cv					2195:2198	R2cv	2195:2198	R2cv = 0.61 vs. 0.53	2195:2214	When only milk FA concentrations were considered in the model, NEFA prediction was more accurate using measurements from evening milk than from morning milk (R2cv = 0.61 vs. 0.53).
31301831	18	8	theme	high	2612:2615	arg1	accuracy					2617:2624	moderately high accuracy	2601:2624	moderately high accuracy	2601:2624	Our findings indicate that ES of cows in early lactation can be monitored with moderately high accuracy by routine milk measurements.
31301831	6	9	theme	dairy	944:948	arg1	cows					950:953	137 Nordic Red dairy cows	929:953	137 Nordic Red dairy cows	929:953	Data were collected from 2 research herds during 2013 to 2016 and included 137 Nordic Red dairy cows, all of which had a first lactation and 59 of which also had a second lactation.
31301831	3	10	theme	acid	551:554	arg1	composition					561:571	milk fatty acid (FA) composition	540:571	milk fatty acid (FA) composition	540:571	Cows' ES can be estimated by calculating the energy balance from energy intake and output and predicted by indicator traits such as change in body weight (ΔBW), change in body condition score (ΔBCS), milk fat:protein ratio (FPR), or milk fatty acid (FA) composition.
31301831	2	11	theme	inexpensive	221:231	arg1	way					233:235	An easy and inexpensive way	209:235	An easy and inexpensive way to monitor postpartum energy status (ES) of cows	209:284	An easy and inexpensive way to monitor postpartum energy status (ES) of cows is therefore needed.
31301831	15	12	theme	milk	2047:2050	arg1	concentrations					2055:2068	only milk FA concentrations	2042:2068	only milk FA concentrations	2042:2068	When only milk FA concentrations were considered in the model, NEFA prediction was more accurate using measurements from evening milk than from morning milk (R2cv = 0.61 vs. 0.53).
31301831	1	13	theme	cow	142:144	arg1	goals					155:159	dairy cow breeding goals	136:159	dairy cow breeding goals	136:159	The inclusion of feed intake and efficiency traits in dairy cow breeding goals can lead to increased risk of metabolic stress.
31301831	6	14	theme	Nordic	933:938	arg1	cows					950:953	137 Nordic Red dairy cows	929:953	137 Nordic Red dairy cows	929:953	Data were collected from 2 research herds during 2013 to 2016 and included 137 Nordic Red dairy cows, all of which had a first lactation and 59 of which also had a second lactation.
31301831	15	15	dep	=	2200:2200	arg1	0.53					2211:2214	0.53	2211:2214	0.53	2211:2214	When only milk FA concentrations were considered in the model, NEFA prediction was more accurate using measurements from evening milk than from morning milk (R2cv = 0.61 vs. 0.53).
31301831	15	15	dep	=	2200:2200	arg1	0.61					2202:2205	0.61	2202:2205	0.61	2202:2205	When only milk FA concentrations were considered in the model, NEFA prediction was more accurate using measurements from evening milk than from morning milk (R2cv = 0.61 vs. 0.53).
31301831	15	16	theme	morning	2181:2187	arg1	milk					2189:2192	morning milk	2181:2192	morning milk (R2cv = 0.61 vs. 0.53)	2181:2215	When only milk FA concentrations were considered in the model, NEFA prediction was more accurate using measurements from evening milk than from morning milk (R2cv = 0.61 vs. 0.53).
31301831	11	17	contain	had	1562:1564	arg1	sum					1541:1543	the sum	1537:1543	the sum of C18:1 in milk	1537:1560	Among individual indicators, C18:1 cis-9 and the sum of C18:1 in milk had the highest correlations (r = 0.73) with NEFA.
31301831	11	17	contain	had	1562:1564	arg2	correlations					1578:1589	the highest correlations	1566:1589	the highest correlations (r = 0.73)	1566:1600	Among individual indicators, C18:1 cis-9 and the sum of C18:1 in milk had the highest correlations (r = 0.73) with NEFA.
31301831	11	17	contain	had	1562:1564	arg1	cis-9					1527:1531	C18:1 cis-9	1521:1531	C18:1 cis-9	1521:1531	Among individual indicators, C18:1 cis-9 and the sum of C18:1 in milk had the highest correlations (r = 0.73) with NEFA.
31301831	3	18	theme	body	478:481	arg1	ΔBCS					500:503	ΔBCS	500:503	ΔBCS	500:503	Cows' ES can be estimated by calculating the energy balance from energy intake and output and predicted by indicator traits such as change in body weight (ΔBW), change in body condition score (ΔBCS), milk fat:protein ratio (FPR), or milk fatty acid (FA) composition.
31301831	3	18	theme	body	478:481	arg1	score					493:497	body condition score	478:497	body condition score (ΔBCS)	478:504	Cows' ES can be estimated by calculating the energy balance from energy intake and output and predicted by indicator traits such as change in body weight (ΔBW), change in body condition score (ΔBCS), milk fat:protein ratio (FPR), or milk fatty acid (FA) composition.
31301831	16	19	theme	milk	2238:2241	arg1	traits					2243:2248	milk traits	2238:2248	milk traits	2238:2248	The best model with milk traits contained FPR, C10:0, C14:0, C18:1 cis-9, C18:1 cis-9 × C14:0, and days in milk (R2cv = 0.62; RMSE = 0.177 mmol/L).
31301831	12	20	theme	NEFA	1657:1660	arg1	prediction					1662:1671	NEFA prediction	1657:1671	NEFA prediction	1657:1671	Seven multiple linear regression models for NEFA prediction were developed using stepwise selection.
31301831	2	21	theme	postpartum	248:257	arg1	ES					274:275	ES	274:275	ES	274:275	An easy and inexpensive way to monitor postpartum energy status (ES) of cows is therefore needed.
31301831	2	21	theme	postpartum	248:257	arg1	status					266:271	postpartum energy status	248:271	postpartum energy status (ES) of cows	248:284	An easy and inexpensive way to monitor postpartum energy status (ES) of cows is therefore needed.
31301831	12	22	theme	multiple	1619:1626	arg1	models					1646:1651	Seven multiple linear regression models	1613:1651	Seven multiple linear regression models for NEFA prediction	1613:1671	Seven multiple linear regression models for NEFA prediction were developed using stepwise selection.
31301831	0	23	from	status	55:60	arg1	lactation					71:79	early lactation	65:79	early lactation	65:79	Body and milk traits as indicators of dairy cow energy status in early lactation.
31301831	16	24	contain	contained	2250:2258	arg2	C18:1					2279:2283	C18:1	2279:2283	C18:1	2279:2283	The best model with milk traits contained FPR, C10:0, C14:0, C18:1 cis-9, C18:1 cis-9 × C14:0, and days in milk (R2cv = 0.62; RMSE = 0.177 mmol/L).
31301831	16	24	contain	contained	2250:2258	arg2	C10:0					2265:2269	C10:0	2265:2269	C10:0	2265:2269	The best model with milk traits contained FPR, C10:0, C14:0, C18:1 cis-9, C18:1 cis-9 × C14:0, and days in milk (R2cv = 0.62; RMSE = 0.177 mmol/L).
31301831	16	24	contain	contained	2250:2258	arg2	days					2317:2320	days	2317:2320	days	2317:2320	The best model with milk traits contained FPR, C10:0, C14:0, C18:1 cis-9, C18:1 cis-9 × C14:0, and days in milk (R2cv = 0.62; RMSE = 0.177 mmol/L).
31301831	16	24	contain	contained	2250:2258	arg1	model					2227:2231	The best model	2218:2231	The best model with milk traits	2218:2248	The best model with milk traits contained FPR, C10:0, C14:0, C18:1 cis-9, C18:1 cis-9 × C14:0, and days in milk (R2cv = 0.62; RMSE = 0.177 mmol/L).
31301831	16	24	contain	contained	2250:2258	arg2	C14:0					2306:2310	C14:0	2306:2310	C14:0	2306:2310	The best model with milk traits contained FPR, C10:0, C14:0, C18:1 cis-9, C18:1 cis-9 × C14:0, and days in milk (R2cv = 0.62; RMSE = 0.177 mmol/L).
31301831	16	24	contain	contained	2250:2258	arg2	FPR					2260:2262	FPR	2260:2262	FPR	2260:2262	The best model with milk traits contained FPR, C10:0, C14:0, C18:1 cis-9, C18:1 cis-9 × C14:0, and days in milk (R2cv = 0.62; RMSE = 0.177 mmol/L).
31301831	17	25	theme	0.176	2507:2511	arg1	mmol/L					2513:2518	0.176 mmol/L	2507:2518	0.176 mmol/L	2507:2518	The most advanced model using both milk and body traits gave a slightly better fit than the model with only milk traits (R2cv = 0.63; RMSE = 0.176 mmol/L).
31301831	1	26	theme	increased	173:181	arg1	risk					183:186	increased risk	173:186	increased risk of metabolic stress	173:206	The inclusion of feed intake and efficiency traits in dairy cow breeding goals can lead to increased risk of metabolic stress.
31301831	8	27	theme	lactation	1166:1174	arg1	wk					1176:1177	lactation wk 2 and 3	1166:1185	wk	1176:1177	Plasma samples for NEFA were collected twice in lactation wk 2 and 3 and once in wk 20.
31301831	7	28	theme	feed	1089:1092	arg1	intake					1094:1099	feed intake	1089:1099	feed intake	1089:1099	The data included daily body weight, milk yield, and feed intake and monthly BCS.
31301831	12	29	theme	linear	1628:1633	arg1	models					1646:1651	Seven multiple linear regression models	1613:1651	Seven multiple linear regression models for NEFA prediction	1613:1671	Seven multiple linear regression models for NEFA prediction were developed using stepwise selection.
31301831	10	30	theme	±	1413:1413	arg1	0.22					1415:1418	0.43 ± 0.22	1408:1418	0.43 ± 0.22	1408:1418	Plasma NEFA concentration was higher in lactation wk 2 and 3 than in wk 20 (0.56 ± 0.30, 0.43 ± 0.22, and 0.13 ± 0.06 mmol/L, respectively; all means ± standard deviation).
31301831	13	31	theme	best	1803:1806	arg1	fit					1808:1810	the best fit	1799:1810	the best fit	1799:1810	Of the models that included milk traits (other than milk FA) as well as body traits, the best fit was achieved by a model with milk yield, FPR, ΔBW, ΔBCS, FPR × ΔBW, and days in milk.
31301831	14	32	theme	mmol/L	2029:2034	arg1	error					2007:2011	a root mean squared error	1987:2011	a root mean squared error (RMSE) of 0.196 mmol/L	1987:2034	The model resulted in a cross-validation coefficient of determination (R2cv) of 0.51 and a root mean squared error (RMSE) of 0.196 mmol/L.
31301831	14	32	theme	mmol/L	2029:2034	arg1	coefficient					1939:1949	a cross-validation coefficient	1920:1949	a cross-validation coefficient of determination (R2cv) of 0.51	1920:1981	The model resulted in a cross-validation coefficient of determination (R2cv) of 0.51 and a root mean squared error (RMSE) of 0.196 mmol/L.
31301831	14	32	theme	mmol/L	2029:2034	arg1	RMSE					2014:2017	RMSE	2014:2017	RMSE	2014:2017	The model resulted in a cross-validation coefficient of determination (R2cv) of 0.51 and a root mean squared error (RMSE) of 0.196 mmol/L.
31301831	11	33	from	cis-9	1527:1531	arg1	milk					1557:1560	milk	1557:1560	milk	1557:1560	Among individual indicators, C18:1 cis-9 and the sum of C18:1 in milk had the highest correlations (r = 0.73) with NEFA.
31301831	13	34	from	days	1884:1887	arg1	milk					1892:1895	milk	1892:1895	milk	1892:1895	Of the models that included milk traits (other than milk FA) as well as body traits, the best fit was achieved by a model with milk yield, FPR, ΔBW, ΔBCS, FPR × ΔBW, and days in milk.
31301831	17	35	dep	traits	2479:2484	arg1	=					2505:2505	=	2505:2505	= 0.176 mmol/L	2505:2518	The most advanced model using both milk and body traits gave a slightly better fit than the model with only milk traits (R2cv = 0.63; RMSE = 0.176 mmol/L).
31301831	2	36	theme	easy	212:215	arg1	way					233:235	An easy and inexpensive way	209:235	An easy and inexpensive way to monitor postpartum energy status (ES) of cows	209:284	An easy and inexpensive way to monitor postpartum energy status (ES) of cows is therefore needed.
31301831	1	37	theme	intake	104:109	arg1	inclusion					86:94	The inclusion	82:94	The inclusion of feed intake and efficiency traits in dairy cow breeding goals	82:159	The inclusion of feed intake and efficiency traits in dairy cow breeding goals can lead to increased risk of metabolic stress.
31301831	5	38	theme	ES	738:739	arg1	indicators					741:750	ES indicators	738:750	ES indicators	738:750	We determined associations between NEFA concentration and ES indicators and evaluated the usefulness of body and milk traits alone, or together, in predicting ES of the cow.
31301831	7	39	theme	milk	1073:1076	arg1	yield					1078:1082	milk yield	1073:1082	milk yield	1073:1082	The data included daily body weight, milk yield, and feed intake and monthly BCS.
31301831	2	40	dep	way	233:235	arg1	monitor					240:246	monitor	240:246	to monitor postpartum energy status (ES) of cows	237:284	An easy and inexpensive way to monitor postpartum energy status (ES) of cows is therefore needed.
31301831	3	41	from	change	468:473	arg1	ΔBCS					500:503	ΔBCS	500:503	ΔBCS	500:503	Cows' ES can be estimated by calculating the energy balance from energy intake and output and predicted by indicator traits such as change in body weight (ΔBW), change in body condition score (ΔBCS), milk fat:protein ratio (FPR), or milk fatty acid (FA) composition.
31301831	3	41	from	change	468:473	arg1	score					493:497	body condition score	478:497	body condition score (ΔBCS)	478:504	Cows' ES can be estimated by calculating the energy balance from energy intake and output and predicted by indicator traits such as change in body weight (ΔBW), change in body condition score (ΔBCS), milk fat:protein ratio (FPR), or milk fatty acid (FA) composition.
31301831	17	42	theme	only	2469:2472	arg1	traits					2479:2484	only milk traits	2469:2484	only milk traits (R2cv = 0.63; RMSE = 0.176 mmol/L)	2469:2519	The most advanced model using both milk and body traits gave a slightly better fit than the model with only milk traits (R2cv = 0.63; RMSE = 0.176 mmol/L).
31301831	0	43	theme	cow	44:46	arg1	status					55:60	dairy cow energy status	38:60	dairy cow energy status in early lactation	38:79	Body and milk traits as indicators of dairy cow energy status in early lactation.
31301831	9	44	theme	Milk	1206:1209	arg1	samples					1211:1217	Milk samples	1206:1217	Milk samples for analysis of fat, protein, lactose, and FA concentrations	1206:1278	Milk samples for analysis of fat, protein, lactose, and FA concentrations were taken on the blood sampling days.
31301831	16	45	dep	=	2349:2349	arg1	R2cv					2331:2334	R2cv	2331:2334	R2cv = 0.62	2331:2341	The best model with milk traits contained FPR, C10:0, C14:0, C18:1 cis-9, C18:1 cis-9 × C14:0, and days in milk (R2cv = 0.62; RMSE = 0.177 mmol/L).
31301831	16	46	dep	=	2336:2336	arg1	0.62					2338:2341	0.62	2338:2341	0.62	2338:2341	The best model with milk traits contained FPR, C10:0, C14:0, C18:1 cis-9, C18:1 cis-9 × C14:0, and days in milk (R2cv = 0.62; RMSE = 0.177 mmol/L).
31301831	0	47	theme	status	55:60	arg1	indicators					24:33	indicators	24:33	indicators of dairy cow energy status in early lactation	24:79	Body and milk traits as indicators of dairy cow energy status in early lactation.
31301831	5	48	theme	NEFA	715:718	arg1	concentration					720:732	NEFA concentration	715:732	NEFA concentration	715:732	We determined associations between NEFA concentration and ES indicators and evaluated the usefulness of body and milk traits alone, or together, in predicting ES of the cow.
31301831	11	49	dep	=	1594:1594	arg1	0.73					1596:1599	0.73	1596:1599	0.73	1596:1599	Among individual indicators, C18:1 cis-9 and the sum of C18:1 in milk had the highest correlations (r = 0.73) with NEFA.
31301831	16	50	with	model	2227:2231	arg1	traits					2243:2248	milk traits	2238:2248	milk traits	2238:2248	The best model with milk traits contained FPR, C10:0, C14:0, C18:1 cis-9, C18:1 cis-9 × C14:0, and days in milk (R2cv = 0.62; RMSE = 0.177 mmol/L).
31301831	0	51	theme	early	65:69	arg1	lactation					71:79	early lactation	65:79	early lactation	65:79	Body and milk traits as indicators of dairy cow energy status in early lactation.
31301831	5	52	theme	body	784:787	arg1	usefulness					770:779	the usefulness	766:779	the usefulness of body and milk traits	766:803	We determined associations between NEFA concentration and ES indicators and evaluated the usefulness of body and milk traits alone, or together, in predicting ES of the cow.
31301831	14	53	theme	mean	1994:1997	arg1	error					2007:2011	a root mean squared error	1987:2011	a root mean squared error (RMSE) of 0.196 mmol/L	1987:2034	The model resulted in a cross-validation coefficient of determination (R2cv) of 0.51 and a root mean squared error (RMSE) of 0.196 mmol/L.
31301831	14	53	theme	mean	1994:1997	arg1	RMSE					2014:2017	RMSE	2014:2017	RMSE	2014:2017	The model resulted in a cross-validation coefficient of determination (R2cv) of 0.51 and a root mean squared error (RMSE) of 0.196 mmol/L.
31301831	3	54	from	composition	561:571	arg1	ΔBCS					500:503	ΔBCS	500:503	ΔBCS	500:503	Cows' ES can be estimated by calculating the energy balance from energy intake and output and predicted by indicator traits such as change in body weight (ΔBW), change in body condition score (ΔBCS), milk fat:protein ratio (FPR), or milk fatty acid (FA) composition.
31301831	3	54	from	composition	561:571	arg1	score					493:497	body condition score	478:497	body condition score (ΔBCS)	478:504	Cows' ES can be estimated by calculating the energy balance from energy intake and output and predicted by indicator traits such as change in body weight (ΔBW), change in body condition score (ΔBCS), milk fat:protein ratio (FPR), or milk fatty acid (FA) composition.
31301831	10	55	theme	Plasma	1319:1324	arg1	concentration					1331:1343	Plasma NEFA concentration	1319:1343	Plasma NEFA concentration	1319:1343	Plasma NEFA concentration was higher in lactation wk 2 and 3 than in wk 20 (0.56 ± 0.30, 0.43 ± 0.22, and 0.13 ± 0.06 mmol/L, respectively; all means ± standard deviation).
31301831	5	56	theme	milk	793:796	arg1	traits					798:803	milk traits	793:803	milk traits	793:803	We determined associations between NEFA concentration and ES indicators and evaluated the usefulness of body and milk traits alone, or together, in predicting ES of the cow.
31301831	18	57	theme	milk	2637:2640	arg1	measurements					2642:2653	routine milk measurements	2629:2653	routine milk measurements	2629:2653	Our findings indicate that ES of cows in early lactation can be monitored with moderately high accuracy by routine milk measurements.
31301831	1	58	from	inclusion	86:94	arg1	goals					155:159	dairy cow breeding goals	136:159	dairy cow breeding goals	136:159	The inclusion of feed intake and efficiency traits in dairy cow breeding goals can lead to increased risk of metabolic stress.
31301831	7	59	theme	body	1060:1063	arg1	weight					1065:1070	daily body weight	1054:1070	daily body weight	1054:1070	The data included daily body weight, milk yield, and feed intake and monthly BCS.
31301831	11	60	theme	C18:1	1548:1552	arg1	sum					1541:1543	the sum	1537:1543	the sum of C18:1 in milk	1537:1560	Among individual indicators, C18:1 cis-9 and the sum of C18:1 in milk had the highest correlations (r = 0.73) with NEFA.
31301831	11	60	theme	C18:1	1548:1552	arg1	cis-9					1527:1531	C18:1 cis-9	1521:1531	C18:1 cis-9	1521:1531	Among individual indicators, C18:1 cis-9 and the sum of C18:1 in milk had the highest correlations (r = 0.73) with NEFA.
31301831	11	61	theme	C18:1	1521:1525	arg1	cis-9					1527:1531	C18:1 cis-9	1521:1531	C18:1 cis-9	1521:1531	Among individual indicators, C18:1 cis-9 and the sum of C18:1 in milk had the highest correlations (r = 0.73) with NEFA.
31301831	15	62	theme	evening	2158:2164	arg1	milk					2166:2169	evening milk	2158:2169	evening milk	2158:2169	When only milk FA concentrations were considered in the model, NEFA prediction was more accurate using measurements from evening milk than from morning milk (R2cv = 0.61 vs. 0.53).
31301831	11	63	dep	correlations	1578:1589	arg1	r					1592:1592	r	1592:1592	r = 0.73	1592:1599	Among individual indicators, C18:1 cis-9 and the sum of C18:1 in milk had the highest correlations (r = 0.73) with NEFA.
31301831	14	64	theme	root	1989:1992	arg1	error					2007:2011	a root mean squared error	1987:2011	a root mean squared error (RMSE) of 0.196 mmol/L	1987:2034	The model resulted in a cross-validation coefficient of determination (R2cv) of 0.51 and a root mean squared error (RMSE) of 0.196 mmol/L.
31301831	14	64	theme	root	1989:1992	arg1	RMSE					2014:2017	RMSE	2014:2017	RMSE	2014:2017	The model resulted in a cross-validation coefficient of determination (R2cv) of 0.51 and a root mean squared error (RMSE) of 0.196 mmol/L.
31301831	3	65	theme	protein	516:522	arg1	FPR					531:533	FPR	531:533	FPR	531:533	Cows' ES can be estimated by calculating the energy balance from energy intake and output and predicted by indicator traits such as change in body weight (ΔBW), change in body condition score (ΔBCS), milk fat:protein ratio (FPR), or milk fatty acid (FA) composition.
31301831	3	65	theme	protein	516:522	arg1	ratio					524:528	protein ratio	516:528	protein ratio (FPR)	516:534	Cows' ES can be estimated by calculating the energy balance from energy intake and output and predicted by indicator traits such as change in body weight (ΔBW), change in body condition score (ΔBCS), milk fat:protein ratio (FPR), or milk fatty acid (FA) composition.
31301831	3	66	theme	energy	372:377	arg1	intake					379:384	energy intake	372:384	energy intake	372:384	Cows' ES can be estimated by calculating the energy balance from energy intake and output and predicted by indicator traits such as change in body weight (ΔBW), change in body condition score (ΔBCS), milk fat:protein ratio (FPR), or milk fatty acid (FA) composition.
31301831	9	67	theme	FA	1262:1263	arg1	concentrations					1265:1278	FA concentrations	1262:1278	FA concentrations	1262:1278	Milk samples for analysis of fat, protein, lactose, and FA concentrations were taken on the blood sampling days.
31301831	10	68	dep	higher	1349:1354	arg1	±					1400:1400	0.56 ± 0.30	1395:1405	0.56 ± 0.30	1395:1405	Plasma NEFA concentration was higher in lactation wk 2 and 3 than in wk 20 (0.56 ± 0.30, 0.43 ± 0.22, and 0.13 ± 0.06 mmol/L, respectively; all means ± standard deviation).
31301831	3	69	from	intake	379:384	arg1	balance					359:365	the energy balance	348:365	the energy balance from energy intake and output	348:395	Cows' ES can be estimated by calculating the energy balance from energy intake and output and predicted by indicator traits such as change in body weight (ΔBW), change in body condition score (ΔBCS), milk fat:protein ratio (FPR), or milk fatty acid (FA) composition.
31301831	2	70	theme	cows	281:284	arg1	ES					274:275	ES	274:275	ES	274:275	An easy and inexpensive way to monitor postpartum energy status (ES) of cows is therefore needed.
31301831	2	70	theme	cows	281:284	arg1	status					266:271	postpartum energy status	248:271	postpartum energy status (ES) of cows	248:284	An easy and inexpensive way to monitor postpartum energy status (ES) of cows is therefore needed.
31301831	16	71	theme	=	2336:2336	arg1	R2cv					2331:2334	R2cv	2331:2334	R2cv = 0.62	2331:2341	The best model with milk traits contained FPR, C10:0, C14:0, C18:1 cis-9, C18:1 cis-9 × C14:0, and days in milk (R2cv = 0.62; RMSE = 0.177 mmol/L).
31301831	13	72	theme	×	1873:1873	arg1	ΔBW					1875:1877	FPR × ΔBW	1869:1877	FPR × ΔBW	1869:1877	Of the models that included milk traits (other than milk FA) as well as body traits, the best fit was achieved by a model with milk yield, FPR, ΔBW, ΔBCS, FPR × ΔBW, and days in milk.
31301831	6	73	contain	had	969:971	arg2	59					995:996	59	995:996	59	995:996	Data were collected from 2 research herds during 2013 to 2016 and included 137 Nordic Red dairy cows, all of which had a first lactation and 59 of which also had a second lactation.
31301831	6	73	contain	had	969:971	arg1	all					956:958	all	956:958	all	956:958	Data were collected from 2 research herds during 2013 to 2016 and included 137 Nordic Red dairy cows, all of which had a first lactation and 59 of which also had a second lactation.
31301831	6	73	contain	had	969:971	arg2	lactation					981:989	a first lactation	973:989	a first lactation	973:989	Data were collected from 2 research herds during 2013 to 2016 and included 137 Nordic Red dairy cows, all of which had a first lactation and 59 of which also had a second lactation.
31301831	6	73	contain	had	969:971	arg1	cows					950:953	137 Nordic Red dairy cows	929:953	137 Nordic Red dairy cows	929:953	Data were collected from 2 research herds during 2013 to 2016 and included 137 Nordic Red dairy cows, all of which had a first lactation and 59 of which also had a second lactation.
31301831	10	74	theme	standard	1471:1478	arg1	deviation					1480:1488	all means ± standard deviation	1459:1488	all means ± standard deviation	1459:1488	Plasma NEFA concentration was higher in lactation wk 2 and 3 than in wk 20 (0.56 ± 0.30, 0.43 ± 0.22, and 0.13 ± 0.06 mmol/L, respectively; all means ± standard deviation).
31301831	4	75	theme	plasma	603:608	arg1	concentration					643:655	blood plasma nonesterified fatty acids (NEFA) concentration	597:655	blood plasma nonesterified fatty acids (NEFA) concentration	597:655	In this study, we used blood plasma nonesterified fatty acids (NEFA) concentration as a biomarker for ES.
31301831	4	75	theme	plasma	603:608	arg1	biomarker					662:670	a biomarker	660:670	a biomarker for ES	660:677	In this study, we used blood plasma nonesterified fatty acids (NEFA) concentration as a biomarker for ES.
31301831	3	76	from	output	390:395	arg1	balance					359:365	the energy balance	348:365	the energy balance from energy intake and output	348:395	Cows' ES can be estimated by calculating the energy balance from energy intake and output and predicted by indicator traits such as change in body weight (ΔBW), change in body condition score (ΔBCS), milk fat:protein ratio (FPR), or milk fatty acid (FA) composition.
31301831	10	77	theme	lactation	1359:1367	arg1	wk					1369:1370	lactation wk 2 and 3	1359:1378	lactation wk 2 and 3	1359:1378	Plasma NEFA concentration was higher in lactation wk 2 and 3 than in wk 20 (0.56 ± 0.30, 0.43 ± 0.22, and 0.13 ± 0.06 mmol/L, respectively; all means ± standard deviation).
31301831	4	78	used	used	592:595	arg2	we					589:590	we	589:590	we	589:590	In this study, we used blood plasma nonesterified fatty acids (NEFA) concentration as a biomarker for ES.
31301831	3	79	theme	body	449:452	arg1	weight					454:459	body weight	449:459	body weight (ΔBW)	449:465	Cows' ES can be estimated by calculating the energy balance from energy intake and output and predicted by indicator traits such as change in body weight (ΔBW), change in body condition score (ΔBCS), milk fat:protein ratio (FPR), or milk fatty acid (FA) composition.
31301831	3	79	theme	body	449:452	arg1	ΔBW					462:464	ΔBW	462:464	ΔBW	462:464	Cows' ES can be estimated by calculating the energy balance from energy intake and output and predicted by indicator traits such as change in body weight (ΔBW), change in body condition score (ΔBCS), milk fat:protein ratio (FPR), or milk fatty acid (FA) composition.
31301831	1	80	theme	efficiency	115:124	arg1	inclusion					86:94	The inclusion	82:94	The inclusion of feed intake and efficiency traits in dairy cow breeding goals	82:159	The inclusion of feed intake and efficiency traits in dairy cow breeding goals can lead to increased risk of metabolic stress.
31301831	3	81	from	ratio	524:528	arg1	ΔBCS					500:503	ΔBCS	500:503	ΔBCS	500:503	Cows' ES can be estimated by calculating the energy balance from energy intake and output and predicted by indicator traits such as change in body weight (ΔBW), change in body condition score (ΔBCS), milk fat:protein ratio (FPR), or milk fatty acid (FA) composition.
31301831	3	81	from	ratio	524:528	arg1	score					493:497	body condition score	478:497	body condition score (ΔBCS)	478:504	Cows' ES can be estimated by calculating the energy balance from energy intake and output and predicted by indicator traits such as change in body weight (ΔBW), change in body condition score (ΔBCS), milk fat:protein ratio (FPR), or milk fatty acid (FA) composition.
31301831	10	82	theme	means	1463:1467	arg1	deviation					1480:1488	all means ± standard deviation	1459:1488	all means ± standard deviation	1459:1488	Plasma NEFA concentration was higher in lactation wk 2 and 3 than in wk 20 (0.56 ± 0.30, 0.43 ± 0.22, and 0.13 ± 0.06 mmol/L, respectively; all means ± standard deviation).
31301831	4	83	theme	fatty	624:628	arg1	NEFA					637:640	NEFA	637:640	NEFA	637:640	In this study, we used blood plasma nonesterified fatty acids (NEFA) concentration as a biomarker for ES.
31301831	4	83	theme	fatty	624:628	arg1	acids					630:634	nonesterified fatty acids	610:634	blood plasma nonesterified fatty acids (NEFA) concentration	597:655	In this study, we used blood plasma nonesterified fatty acids (NEFA) concentration as a biomarker for ES.
31301831	13	84	theme	milk	1841:1844	arg1	yield					1846:1850	milk yield	1841:1850	milk yield	1841:1850	Of the models that included milk traits (other than milk FA) as well as body traits, the best fit was achieved by a model with milk yield, FPR, ΔBW, ΔBCS, FPR × ΔBW, and days in milk.
31301831	1	85	theme	metabolic	191:199	arg1	stress					201:206	metabolic stress	191:206	metabolic stress	191:206	The inclusion of feed intake and efficiency traits in dairy cow breeding goals can lead to increased risk of metabolic stress.
31301831	14	86	theme	determination	1954:1966	arg1	error					2007:2011	a root mean squared error	1987:2011	a root mean squared error (RMSE) of 0.196 mmol/L	1987:2034	The model resulted in a cross-validation coefficient of determination (R2cv) of 0.51 and a root mean squared error (RMSE) of 0.196 mmol/L.
31301831	14	86	theme	determination	1954:1966	arg1	coefficient					1939:1949	a cross-validation coefficient	1920:1949	a cross-validation coefficient of determination (R2cv) of 0.51	1920:1981	The model resulted in a cross-validation coefficient of determination (R2cv) of 0.51 and a root mean squared error (RMSE) of 0.196 mmol/L.
31301831	14	86	theme	determination	1954:1966	arg1	RMSE					2014:2017	RMSE	2014:2017	RMSE	2014:2017	The model resulted in a cross-validation coefficient of determination (R2cv) of 0.51 and a root mean squared error (RMSE) of 0.196 mmol/L.
31301831	9	87	theme	blood	1298:1302	arg1	days					1313:1316	the blood sampling days	1294:1316	the blood sampling days	1294:1316	Milk samples for analysis of fat, protein, lactose, and FA concentrations were taken on the blood sampling days.
31301831	6	88	theme	research	881:888	arg1	herds					890:894	2 research herds	879:894	2 research herds	879:894	Data were collected from 2 research herds during 2013 to 2016 and included 137 Nordic Red dairy cows, all of which had a first lactation and 59 of which also had a second lactation.
31301831	13	89	theme	body	1786:1789	arg1	traits					1791:1796	body traits	1786:1796	milk traits (other than milk FA) as well as body traits	1742:1796	Of the models that included milk traits (other than milk FA) as well as body traits, the best fit was achieved by a model with milk yield, FPR, ΔBW, ΔBCS, FPR × ΔBW, and days in milk.
31301831	3	90	theme	fatty	545:549	arg1	composition					561:571	milk fatty acid (FA) composition	540:571	milk fatty acid (FA) composition	540:571	Cows' ES can be estimated by calculating the energy balance from energy intake and output and predicted by indicator traits such as change in body weight (ΔBW), change in body condition score (ΔBCS), milk fat:protein ratio (FPR), or milk fatty acid (FA) composition.
31301831	17	91	theme	body	2410:2413	arg1	traits					2415:2420	both milk and body traits	2396:2420	traits	2415:2420	The most advanced model using both milk and body traits gave a slightly better fit than the model with only milk traits (R2cv = 0.63; RMSE = 0.176 mmol/L).
31301831	16	92	from	days	2317:2320	arg1	milk					2325:2328	milk	2325:2328	milk	2325:2328	The best model with milk traits contained FPR, C10:0, C14:0, C18:1 cis-9, C18:1 cis-9 × C14:0, and days in milk (R2cv = 0.62; RMSE = 0.177 mmol/L).
31301831	13	93	with	model	1830:1834	arg1	days					1884:1887	days	1884:1887	days	1884:1887	Of the models that included milk traits (other than milk FA) as well as body traits, the best fit was achieved by a model with milk yield, FPR, ΔBW, ΔBCS, FPR × ΔBW, and days in milk.
31301831	13	93	with	model	1830:1834	arg1	yield					1846:1850	milk yield	1841:1850	milk yield	1841:1850	Of the models that included milk traits (other than milk FA) as well as body traits, the best fit was achieved by a model with milk yield, FPR, ΔBW, ΔBCS, FPR × ΔBW, and days in milk.
31301831	13	93	with	model	1830:1834	arg1	ΔBCS					1863:1866	ΔBCS	1863:1866	ΔBCS	1863:1866	Of the models that included milk traits (other than milk FA) as well as body traits, the best fit was achieved by a model with milk yield, FPR, ΔBW, ΔBCS, FPR × ΔBW, and days in milk.
31301831	13	93	with	model	1830:1834	arg1	FPR					1853:1855	FPR	1853:1855	FPR	1853:1855	Of the models that included milk traits (other than milk FA) as well as body traits, the best fit was achieved by a model with milk yield, FPR, ΔBW, ΔBCS, FPR × ΔBW, and days in milk.
31301831	13	93	with	model	1830:1834	arg1	ΔBW					1858:1860	ΔBW	1858:1860	ΔBW	1858:1860	Of the models that included milk traits (other than milk FA) as well as body traits, the best fit was achieved by a model with milk yield, FPR, ΔBW, ΔBCS, FPR × ΔBW, and days in milk.
31301831	13	93	with	model	1830:1834	arg1	ΔBW					1875:1877	FPR × ΔBW	1869:1877	FPR × ΔBW	1869:1877	Of the models that included milk traits (other than milk FA) as well as body traits, the best fit was achieved by a model with milk yield, FPR, ΔBW, ΔBCS, FPR × ΔBW, and days in milk.
31301831	13	94	theme	FPR	1869:1871	arg1	ΔBW					1875:1877	FPR × ΔBW	1869:1877	FPR × ΔBW	1869:1877	Of the models that included milk traits (other than milk FA) as well as body traits, the best fit was achieved by a model with milk yield, FPR, ΔBW, ΔBCS, FPR × ΔBW, and days in milk.
31301831	13	95	dep	traits	1747:1752	arg1	other					1755:1759	other	1755:1759	other	1755:1759	Of the models that included milk traits (other than milk FA) as well as body traits, the best fit was achieved by a model with milk yield, FPR, ΔBW, ΔBCS, FPR × ΔBW, and days in milk.
31301831	18	96	theme	early	2563:2567	arg1	lactation					2569:2577	early lactation	2563:2577	early lactation	2563:2577	Our findings indicate that ES of cows in early lactation can be monitored with moderately high accuracy by routine milk measurements.
31301831	3	97	from	change	439:444	arg1	fat					512:514	milk fat	507:514	milk fat	507:514	Cows' ES can be estimated by calculating the energy balance from energy intake and output and predicted by indicator traits such as change in body weight (ΔBW), change in body condition score (ΔBCS), milk fat:protein ratio (FPR), or milk fatty acid (FA) composition.
31301831	3	97	from	change	439:444	arg1	ΔBW					462:464	ΔBW	462:464	ΔBW	462:464	Cows' ES can be estimated by calculating the energy balance from energy intake and output and predicted by indicator traits such as change in body weight (ΔBW), change in body condition score (ΔBCS), milk fat:protein ratio (FPR), or milk fatty acid (FA) composition.
31301831	3	97	from	change	439:444	arg1	change					468:473	change	468:473	change in body condition score (ΔBCS)	468:504	Cows' ES can be estimated by calculating the energy balance from energy intake and output and predicted by indicator traits such as change in body weight (ΔBW), change in body condition score (ΔBCS), milk fat:protein ratio (FPR), or milk fatty acid (FA) composition.
31301831	3	97	from	change	439:444	arg1	composition					561:571	milk fatty acid (FA) composition	540:571	milk fatty acid (FA) composition	540:571	Cows' ES can be estimated by calculating the energy balance from energy intake and output and predicted by indicator traits such as change in body weight (ΔBW), change in body condition score (ΔBCS), milk fat:protein ratio (FPR), or milk fatty acid (FA) composition.
31301831	3	97	from	change	439:444	arg1	ratio					524:528	protein ratio	516:528	protein ratio (FPR)	516:534	Cows' ES can be estimated by calculating the energy balance from energy intake and output and predicted by indicator traits such as change in body weight (ΔBW), change in body condition score (ΔBCS), milk fat:protein ratio (FPR), or milk fatty acid (FA) composition.
31301831	3	97	from	change	439:444	arg1	weight					454:459	body weight	449:459	body weight (ΔBW)	449:465	Cows' ES can be estimated by calculating the energy balance from energy intake and output and predicted by indicator traits such as change in body weight (ΔBW), change in body condition score (ΔBCS), milk fat:protein ratio (FPR), or milk fatty acid (FA) composition.
31301831	3	97	from	change	439:444	arg1	FPR					531:533	FPR	531:533	FPR	531:533	Cows' ES can be estimated by calculating the energy balance from energy intake and output and predicted by indicator traits such as change in body weight (ΔBW), change in body condition score (ΔBCS), milk fat:protein ratio (FPR), or milk fatty acid (FA) composition.
31301831	1	98	theme	dairy	136:140	arg1	goals					155:159	dairy cow breeding goals	136:159	dairy cow breeding goals	136:159	The inclusion of feed intake and efficiency traits in dairy cow breeding goals can lead to increased risk of metabolic stress.
31301831	6	99	theme	Red	940:942	arg1	cows					950:953	137 Nordic Red dairy cows	929:953	137 Nordic Red dairy cows	929:953	Data were collected from 2 research herds during 2013 to 2016 and included 137 Nordic Red dairy cows, all of which had a first lactation and 59 of which also had a second lactation.
31301831	15	100	theme	NEFA	2100:2103	arg1	prediction					2105:2114	NEFA prediction	2100:2114	NEFA prediction	2100:2114	When only milk FA concentrations were considered in the model, NEFA prediction was more accurate using measurements from evening milk than from morning milk (R2cv = 0.61 vs. 0.53).
31301831	10	101	theme	0.43	1408:1411	arg1	0.22					1415:1418	0.43 ± 0.22	1408:1418	0.43 ± 0.22	1408:1418	Plasma NEFA concentration was higher in lactation wk 2 and 3 than in wk 20 (0.56 ± 0.30, 0.43 ± 0.22, and 0.13 ± 0.06 mmol/L, respectively; all means ± standard deviation).
31301831	15	102	theme	FA	2052:2053	arg1	concentrations					2055:2068	only milk FA concentrations	2042:2068	only milk FA concentrations	2042:2068	When only milk FA concentrations were considered in the model, NEFA prediction was more accurate using measurements from evening milk than from morning milk (R2cv = 0.61 vs. 0.53).
31301831	1	103	theme	breeding	146:153	arg1	goals					155:159	dairy cow breeding goals	136:159	dairy cow breeding goals	136:159	The inclusion of feed intake and efficiency traits in dairy cow breeding goals can lead to increased risk of metabolic stress.
31301831	16	104	theme	best	2222:2225	arg1	model					2227:2231	The best model	2218:2231	The best model with milk traits	2218:2248	The best model with milk traits contained FPR, C10:0, C14:0, C18:1 cis-9, C18:1 cis-9 × C14:0, and days in milk (R2cv = 0.62; RMSE = 0.177 mmol/L).
31301831	12	105	theme	stepwise	1694:1701	arg1	selection					1703:1711	stepwise selection	1694:1711	stepwise selection	1694:1711	Seven multiple linear regression models for NEFA prediction were developed using stepwise selection.
31301831	2	106	theme	energy	259:264	arg1	ES					274:275	ES	274:275	ES	274:275	An easy and inexpensive way to monitor postpartum energy status (ES) of cows is therefore needed.
31301831	2	106	theme	energy	259:264	arg1	status					266:271	postpartum energy status	248:271	postpartum energy status (ES) of cows	248:284	An easy and inexpensive way to monitor postpartum energy status (ES) of cows is therefore needed.
31301831	15	107	theme	only	2042:2045	arg1	concentrations					2055:2068	only milk FA concentrations	2042:2068	only milk FA concentrations	2042:2068	When only milk FA concentrations were considered in the model, NEFA prediction was more accurate using measurements from evening milk than from morning milk (R2cv = 0.61 vs. 0.53).
31301831	11	108	from	sum	1541:1543	arg1	milk					1557:1560	milk	1557:1560	milk	1557:1560	Among individual indicators, C18:1 cis-9 and the sum of C18:1 in milk had the highest correlations (r = 0.73) with NEFA.
31301831	3	109	theme	condition	483:491	arg1	ΔBCS					500:503	ΔBCS	500:503	ΔBCS	500:503	Cows' ES can be estimated by calculating the energy balance from energy intake and output and predicted by indicator traits such as change in body weight (ΔBW), change in body condition score (ΔBCS), milk fat:protein ratio (FPR), or milk fatty acid (FA) composition.
31301831	3	109	theme	condition	483:491	arg1	score					493:497	body condition score	478:497	body condition score (ΔBCS)	478:504	Cows' ES can be estimated by calculating the energy balance from energy intake and output and predicted by indicator traits such as change in body weight (ΔBW), change in body condition score (ΔBCS), milk fat:protein ratio (FPR), or milk fatty acid (FA) composition.
31301831	18	110	from	ES	2549:2550	arg1	lactation					2569:2577	early lactation	2563:2577	early lactation	2563:2577	Our findings indicate that ES of cows in early lactation can be monitored with moderately high accuracy by routine milk measurements.
31301831	7	111	theme	monthly	1105:1111	arg1	BCS					1113:1115	monthly BCS	1105:1115	monthly BCS	1105:1115	The data included daily body weight, milk yield, and feed intake and monthly BCS.
31301831	15	112	dep	milk	2189:2192	arg1	R2cv					2195:2198	R2cv	2195:2198	R2cv = 0.61 vs. 0.53	2195:2214	When only milk FA concentrations were considered in the model, NEFA prediction was more accurate using measurements from evening milk than from morning milk (R2cv = 0.61 vs. 0.53).
31301831	16	113	from	FPR	2260:2262	arg1	milk					2325:2328	milk	2325:2328	milk	2325:2328	The best model with milk traits contained FPR, C10:0, C14:0, C18:1 cis-9, C18:1 cis-9 × C14:0, and days in milk (R2cv = 0.62; RMSE = 0.177 mmol/L).
31301831	18	114	theme	cows	2555:2558	arg1	ES					2549:2550	ES	2549:2550	ES of cows in early lactation	2549:2577	Our findings indicate that ES of cows in early lactation can be monitored with moderately high accuracy by routine milk measurements.
31301831	10	115	dep	±	1400:1400	arg1	mmol/L					1437:1442	0.13 ± 0.06 mmol/L	1425:1442	0.13 ± 0.06 mmol/L	1425:1442	Plasma NEFA concentration was higher in lactation wk 2 and 3 than in wk 20 (0.56 ± 0.30, 0.43 ± 0.22, and 0.13 ± 0.06 mmol/L, respectively; all means ± standard deviation).
31301831	10	115	dep	±	1400:1400	arg1	0.22					1415:1418	0.43 ± 0.22	1408:1418	0.43 ± 0.22	1408:1418	Plasma NEFA concentration was higher in lactation wk 2 and 3 than in wk 20 (0.56 ± 0.30, 0.43 ± 0.22, and 0.13 ± 0.06 mmol/L, respectively; all means ± standard deviation).
31301831	10	115	dep	±	1400:1400	arg1	deviation					1480:1488	all means ± standard deviation	1459:1488	all means ± standard deviation	1459:1488	Plasma NEFA concentration was higher in lactation wk 2 and 3 than in wk 20 (0.56 ± 0.30, 0.43 ± 0.22, and 0.13 ± 0.06 mmol/L, respectively; all means ± standard deviation).
31301831	1	116	theme	feed	99:102	arg1	intake					104:109	feed intake	99:109	feed intake	99:109	The inclusion of feed intake and efficiency traits in dairy cow breeding goals can lead to increased risk of metabolic stress.
31301831	0	117	from	lactation	71:79	arg1	indicators					24:33	indicators	24:33	indicators of dairy cow energy status in early lactation	24:79	Body and milk traits as indicators of dairy cow energy status in early lactation.
31301831	12	118	theme	regression	1635:1644	arg1	models					1646:1651	Seven multiple linear regression models	1613:1651	Seven multiple linear regression models for NEFA prediction	1613:1671	Seven multiple linear regression models for NEFA prediction were developed using stepwise selection.
31301831	13	119	from	ΔBW	1875:1877	arg1	milk					1892:1895	milk	1892:1895	milk	1892:1895	Of the models that included milk traits (other than milk FA) as well as body traits, the best fit was achieved by a model with milk yield, FPR, ΔBW, ΔBCS, FPR × ΔBW, and days in milk.
31301831	0	120	theme	energy	48:53	arg1	status					55:60	dairy cow energy status	38:60	dairy cow energy status in early lactation	38:79	Body and milk traits as indicators of dairy cow energy status in early lactation.
31301831	5	121	theme	cow	849:851	arg1	ES					839:840	ES	839:840	ES of the cow	839:851	We determined associations between NEFA concentration and ES indicators and evaluated the usefulness of body and milk traits alone, or together, in predicting ES of the cow.
31301831	10	122	theme	±	1430:1430	arg1	mmol/L					1437:1442	0.13 ± 0.06 mmol/L	1425:1442	0.13 ± 0.06 mmol/L	1425:1442	Plasma NEFA concentration was higher in lactation wk 2 and 3 than in wk 20 (0.56 ± 0.30, 0.43 ± 0.22, and 0.13 ± 0.06 mmol/L, respectively; all means ± standard deviation).
31301831	17	123	theme	R2cv	2487:2490	arg1	0.63					2494:2497	R2cv = 0.63	2487:2497	R2cv = 0.63	2487:2497	The most advanced model using both milk and body traits gave a slightly better fit than the model with only milk traits (R2cv = 0.63; RMSE = 0.176 mmol/L).
31301831	17	124	with	model	2458:2462	arg1	traits					2479:2484	only milk traits	2469:2484	only milk traits (R2cv = 0.63; RMSE = 0.176 mmol/L)	2469:2519	The most advanced model using both milk and body traits gave a slightly better fit than the model with only milk traits (R2cv = 0.63; RMSE = 0.176 mmol/L).
31301831	17	125	theme	milk	2474:2477	arg1	traits					2479:2484	only milk traits	2469:2484	only milk traits (R2cv = 0.63; RMSE = 0.176 mmol/L)	2469:2519	The most advanced model using both milk and body traits gave a slightly better fit than the model with only milk traits (R2cv = 0.63; RMSE = 0.176 mmol/L).
31301831	17	126	dep	=	2505:2505	arg1	0.63					2494:2497	R2cv = 0.63	2487:2497	R2cv = 0.63	2487:2497	The most advanced model using both milk and body traits gave a slightly better fit than the model with only milk traits (R2cv = 0.63; RMSE = 0.176 mmol/L).
31301831	3	127	from	weight	454:459	arg1	ΔBCS					500:503	ΔBCS	500:503	ΔBCS	500:503	Cows' ES can be estimated by calculating the energy balance from energy intake and output and predicted by indicator traits such as change in body weight (ΔBW), change in body condition score (ΔBCS), milk fat:protein ratio (FPR), or milk fatty acid (FA) composition.
31301831	3	127	from	weight	454:459	arg1	score					493:497	body condition score	478:497	body condition score (ΔBCS)	478:504	Cows' ES can be estimated by calculating the energy balance from energy intake and output and predicted by indicator traits such as change in body weight (ΔBW), change in body condition score (ΔBCS), milk fat:protein ratio (FPR), or milk fatty acid (FA) composition.
31301831	13	128	theme	milk	1742:1745	arg1	traits					1747:1752	milk traits	1742:1752	milk traits (other than milk FA) as well as body traits	1742:1796	Of the models that included milk traits (other than milk FA) as well as body traits, the best fit was achieved by a model with milk yield, FPR, ΔBW, ΔBCS, FPR × ΔBW, and days in milk.
31301831	11	129	theme	=	1594:1594	arg1	r					1592:1592	r	1592:1592	r = 0.73	1592:1599	Among individual indicators, C18:1 cis-9 and the sum of C18:1 in milk had the highest correlations (r = 0.73) with NEFA.
31301831	14	130	theme	squared	1999:2005	arg1	error					2007:2011	a root mean squared error	1987:2011	a root mean squared error (RMSE) of 0.196 mmol/L	1987:2034	The model resulted in a cross-validation coefficient of determination (R2cv) of 0.51 and a root mean squared error (RMSE) of 0.196 mmol/L.
31301831	14	130	theme	squared	1999:2005	arg1	RMSE					2014:2017	RMSE	2014:2017	RMSE	2014:2017	The model resulted in a cross-validation coefficient of determination (R2cv) of 0.51 and a root mean squared error (RMSE) of 0.196 mmol/L.
31301831	1	131	dep	intake	104:109	arg1	traits					126:131	traits	126:131	traits	126:131	The inclusion of feed intake and efficiency traits in dairy cow breeding goals can lead to increased risk of metabolic stress.
31301831	13	132	from	ΔBW	1858:1860	arg1	milk					1892:1895	milk	1892:1895	milk	1892:1895	Of the models that included milk traits (other than milk FA) as well as body traits, the best fit was achieved by a model with milk yield, FPR, ΔBW, ΔBCS, FPR × ΔBW, and days in milk.
31301831	17	133	theme	=	2492:2492	arg1	0.63					2494:2497	R2cv = 0.63	2487:2497	R2cv = 0.63	2487:2497	The most advanced model using both milk and body traits gave a slightly better fit than the model with only milk traits (R2cv = 0.63; RMSE = 0.176 mmol/L).
31301831	9	134	theme	fat	1235:1237	arg1	analysis					1223:1230	analysis	1223:1230	analysis of fat, protein, lactose, and FA concentrations	1223:1278	Milk samples for analysis of fat, protein, lactose, and FA concentrations were taken on the blood sampling days.
31301831	16	135	from	C10:0	2265:2269	arg1	milk					2325:2328	milk	2325:2328	milk	2325:2328	The best model with milk traits contained FPR, C10:0, C14:0, C18:1 cis-9, C18:1 cis-9 × C14:0, and days in milk (R2cv = 0.62; RMSE = 0.177 mmol/L).
31301831	0	136	from	indicators	24:33	arg1	lactation					71:79	early lactation	65:79	early lactation	65:79	Body and milk traits as indicators of dairy cow energy status in early lactation.
31301831	13	137	from	FPR	1853:1855	arg1	milk					1892:1895	milk	1892:1895	milk	1892:1895	Of the models that included milk traits (other than milk FA) as well as body traits, the best fit was achieved by a model with milk yield, FPR, ΔBW, ΔBCS, FPR × ΔBW, and days in milk.
31301831	11	138	theme	individual	1498:1507	arg1	indicators					1509:1518	individual indicators	1498:1518	individual indicators	1498:1518	Among individual indicators, C18:1 cis-9 and the sum of C18:1 in milk had the highest correlations (r = 0.73) with NEFA.
31301831	9	139	theme	protein	1240:1246	arg1	analysis					1223:1230	analysis	1223:1230	analysis of fat, protein, lactose, and FA concentrations	1223:1278	Milk samples for analysis of fat, protein, lactose, and FA concentrations were taken on the blood sampling days.
31301831	7	140	theme	daily	1054:1058	arg1	weight					1065:1070	daily body weight	1054:1070	daily body weight	1054:1070	The data included daily body weight, milk yield, and feed intake and monthly BCS.
31301831	18	141	theme	routine	2629:2635	arg1	measurements					2642:2653	routine milk measurements	2629:2653	routine milk measurements	2629:2653	Our findings indicate that ES of cows in early lactation can be monitored with moderately high accuracy by routine milk measurements.
31301831	16	142	dep	C18:1	2279:2283	arg1	cis-9					2285:2289	cis-9	2285:2289	C14:0, C18:1 cis-9	2272:2289	The best model with milk traits contained FPR, C10:0, C14:0, C18:1 cis-9, C18:1 cis-9 × C14:0, and days in milk (R2cv = 0.62; RMSE = 0.177 mmol/L).
31301831	3	143	theme	energy	352:357	arg1	balance					359:365	the energy balance	348:365	the energy balance from energy intake and output	348:395	Cows' ES can be estimated by calculating the energy balance from energy intake and output and predicted by indicator traits such as change in body weight (ΔBW), change in body condition score (ΔBCS), milk fat:protein ratio (FPR), or milk fatty acid (FA) composition.
31301831	9	144	theme	lactose	1249:1255	arg1	analysis					1223:1230	analysis	1223:1230	analysis of fat, protein, lactose, and FA concentrations	1223:1278	Milk samples for analysis of fat, protein, lactose, and FA concentrations were taken on the blood sampling days.
31301831	15	145	from	milk	2189:2192	arg1	measurements					2140:2151	measurements	2140:2151	measurements from evening milk than from morning milk (R2cv = 0.61 vs. 0.53)	2140:2215	When only milk FA concentrations were considered in the model, NEFA prediction was more accurate using measurements from evening milk than from morning milk (R2cv = 0.61 vs. 0.53).
31301831	10	146	dep	wk	1369:1370	arg1	3					1378:1378	3	1378:1378	3	1378:1378	Plasma NEFA concentration was higher in lactation wk 2 and 3 than in wk 20 (0.56 ± 0.30, 0.43 ± 0.22, and 0.13 ± 0.06 mmol/L, respectively; all means ± standard deviation).
31301831	10	146	dep	wk	1369:1370	arg1	2					1372:1372	2	1372:1372	2	1372:1372	Plasma NEFA concentration was higher in lactation wk 2 and 3 than in wk 20 (0.56 ± 0.30, 0.43 ± 0.22, and 0.13 ± 0.06 mmol/L, respectively; all means ± standard deviation).
31301831	13	147	from	ΔBCS	1863:1866	arg1	milk					1892:1895	milk	1892:1895	milk	1892:1895	Of the models that included milk traits (other than milk FA) as well as body traits, the best fit was achieved by a model with milk yield, FPR, ΔBW, ΔBCS, FPR × ΔBW, and days in milk.
31301831	3	148	theme	milk	507:510	arg1	fat					512:514	milk fat	507:514	milk fat	507:514	Cows' ES can be estimated by calculating the energy balance from energy intake and output and predicted by indicator traits such as change in body weight (ΔBW), change in body condition score (ΔBCS), milk fat:protein ratio (FPR), or milk fatty acid (FA) composition.
31301831	4	149	theme	acids	630:634	arg1	concentration					643:655	blood plasma nonesterified fatty acids (NEFA) concentration	597:655	blood plasma nonesterified fatty acids (NEFA) concentration	597:655	In this study, we used blood plasma nonesterified fatty acids (NEFA) concentration as a biomarker for ES.
31301831	4	149	theme	acids	630:634	arg1	biomarker					662:670	a biomarker	660:670	a biomarker for ES	660:677	In this study, we used blood plasma nonesterified fatty acids (NEFA) concentration as a biomarker for ES.
31301831	10	150	theme	NEFA	1326:1329	arg1	concentration					1331:1343	Plasma NEFA concentration	1319:1343	Plasma NEFA concentration	1319:1343	Plasma NEFA concentration was higher in lactation wk 2 and 3 than in wk 20 (0.56 ± 0.30, 0.43 ± 0.22, and 0.13 ± 0.06 mmol/L, respectively; all means ± standard deviation).
31301831	0	151	theme	dairy	38:42	arg1	status					55:60	dairy cow energy status	38:60	dairy cow energy status in early lactation	38:79	Body and milk traits as indicators of dairy cow energy status in early lactation.
31301831	14	152	theme	0.51	1978:1981	arg1	R2cv					1969:1972	R2cv	1969:1972	R2cv	1969:1972	The model resulted in a cross-validation coefficient of determination (R2cv) of 0.51 and a root mean squared error (RMSE) of 0.196 mmol/L.
31301831	14	152	theme	0.51	1978:1981	arg1	determination					1954:1966	determination	1954:1966	determination (R2cv) of 0.51	1954:1981	The model resulted in a cross-validation coefficient of determination (R2cv) of 0.51 and a root mean squared error (RMSE) of 0.196 mmol/L.
31301831	9	153	theme	concentrations	1265:1278	arg1	analysis					1223:1230	analysis	1223:1230	analysis of fat, protein, lactose, and FA concentrations	1223:1278	Milk samples for analysis of fat, protein, lactose, and FA concentrations were taken on the blood sampling days.
31301831	15	154	from	milk	2166:2169	arg1	measurements					2140:2151	measurements	2140:2151	measurements from evening milk than from morning milk (R2cv = 0.61 vs. 0.53)	2140:2215	When only milk FA concentrations were considered in the model, NEFA prediction was more accurate using measurements from evening milk than from morning milk (R2cv = 0.61 vs. 0.53).
31301831	17	155	theme	better	2438:2443	arg1	fit					2445:2447	a slightly better fit	2427:2447	a slightly better fit	2427:2447	The most advanced model using both milk and body traits gave a slightly better fit than the model with only milk traits (R2cv = 0.63; RMSE = 0.176 mmol/L).
31301831	8	156	theme	Plasma	1118:1123	arg1	samples					1125:1131	Plasma samples	1118:1131	Plasma samples for NEFA	1118:1140	Plasma samples for NEFA were collected twice in lactation wk 2 and 3 and once in wk 20.
31301831	3	157	theme	indicator	414:422	arg1	traits					424:429	indicator traits	414:429	indicator traits such as change in body weight (ΔBW), change in body condition score (ΔBCS), milk fat:protein ratio (FPR), or milk fatty acid (FA) composition	414:571	Cows' ES can be estimated by calculating the energy balance from energy intake and output and predicted by indicator traits such as change in body weight (ΔBW), change in body condition score (ΔBCS), milk fat:protein ratio (FPR), or milk fatty acid (FA) composition.
31301831	3	157	theme	indicator	414:422	arg1	change					439:444	change	439:444	change in body weight (ΔBW), change in body condition score (ΔBCS), milk fat:protein ratio (FPR), or milk fatty acid (FA) composition	439:571	Cows' ES can be estimated by calculating the energy balance from energy intake and output and predicted by indicator traits such as change in body weight (ΔBW), change in body condition score (ΔBCS), milk fat:protein ratio (FPR), or milk fatty acid (FA) composition.
31301831	13	158	theme	milk	1766:1769	arg1	FA					1771:1772	milk FA	1766:1772	milk FA	1766:1772	Of the models that included milk traits (other than milk FA) as well as body traits, the best fit was achieved by a model with milk yield, FPR, ΔBW, ΔBCS, FPR × ΔBW, and days in milk.
31301831	5	159	theme	traits	798:803	arg1	usefulness					770:779	the usefulness	766:779	the usefulness of body and milk traits	766:803	We determined associations between NEFA concentration and ES indicators and evaluated the usefulness of body and milk traits alone, or together, in predicting ES of the cow.
31301831	11	160	theme	highest	1570:1576	arg1	correlations					1578:1589	the highest correlations	1566:1589	the highest correlations (r = 0.73)	1566:1600	Among individual indicators, C18:1 cis-9 and the sum of C18:1 in milk had the highest correlations (r = 0.73) with NEFA.
31301831	10	161	theme	±	1469:1469	arg1	deviation					1480:1488	all means ± standard deviation	1459:1488	all means ± standard deviation	1459:1488	Plasma NEFA concentration was higher in lactation wk 2 and 3 than in wk 20 (0.56 ± 0.30, 0.43 ± 0.22, and 0.13 ± 0.06 mmol/L, respectively; all means ± standard deviation).
31301831	4	162	theme	blood	597:601	arg1	concentration					643:655	blood plasma nonesterified fatty acids (NEFA) concentration	597:655	blood plasma nonesterified fatty acids (NEFA) concentration	597:655	In this study, we used blood plasma nonesterified fatty acids (NEFA) concentration as a biomarker for ES.
31301831	4	162	theme	blood	597:601	arg1	biomarker					662:670	a biomarker	660:670	a biomarker for ES	660:677	In this study, we used blood plasma nonesterified fatty acids (NEFA) concentration as a biomarker for ES.
31301831	13	163	from	yield	1846:1850	arg1	milk					1892:1895	milk	1892:1895	milk	1892:1895	Of the models that included milk traits (other than milk FA) as well as body traits, the best fit was achieved by a model with milk yield, FPR, ΔBW, ΔBCS, FPR × ΔBW, and days in milk.
31301831	3	164	theme	milk	540:543	arg1	composition					561:571	milk fatty acid (FA) composition	540:571	milk fatty acid (FA) composition	540:571	Cows' ES can be estimated by calculating the energy balance from energy intake and output and predicted by indicator traits such as change in body weight (ΔBW), change in body condition score (ΔBCS), milk fat:protein ratio (FPR), or milk fatty acid (FA) composition.
31301831	4	165	theme	nonesterified	610:622	arg1	NEFA					637:640	NEFA	637:640	NEFA	637:640	In this study, we used blood plasma nonesterified fatty acids (NEFA) concentration as a biomarker for ES.
31301831	4	165	theme	nonesterified	610:622	arg1	acids					630:634	nonesterified fatty acids	610:634	blood plasma nonesterified fatty acids (NEFA) concentration	597:655	In this study, we used blood plasma nonesterified fatty acids (NEFA) concentration as a biomarker for ES.
31301831	1	166	theme	stress	201:206	arg1	risk					183:186	increased risk	173:186	increased risk of metabolic stress	173:206	The inclusion of feed intake and efficiency traits in dairy cow breeding goals can lead to increased risk of metabolic stress.
31543446	0	0	from	Sorting	20:26	arg1	Apparatus					104:112	the Golgi Apparatus	94:112	the Golgi Apparatus	94:112	Syndecan-1 Mediates Sorting of Soluble Lipoprotein Lipase with Sphingomyelin-Rich Membrane in the Golgi Apparatus.
31543446	1	1	theme	vesicular	152:160	arg1	carriers					172:179	vesicular transport carriers	152:179	vesicular transport carriers from the trans-Golgi network (TGN)	152:214	In the secretory pathway, budding of vesicular transport carriers from the trans-Golgi network (TGN) must coordinate specification of lipid composition with selection of secreted proteins.
31543446	1	2	theme	lipid	249:253	arg1	composition					255:265	lipid composition	249:265	lipid composition	249:265	In the secretory pathway, budding of vesicular transport carriers from the trans-Golgi network (TGN) must coordinate specification of lipid composition with selection of secreted proteins.
31543446	2	3	theme	sorting	485:491	arg1	receptor					493:500	a sorting receptor	483:500	a sorting receptor	483:500	We elucidate a mechanism of soluble protein cargo sorting into secretory vesicles with a sphingomyelin-rich membrane; the integral membrane proteoglycan Syndecan-1 (SDC1) acts as a sorting receptor, capturing the soluble enzyme lipoprotein lipase (LPL) during export from the TGN.
31543446	2	3	theme	sorting	485:491	arg1	Syndecan-1					457:466	the integral membrane proteoglycan Syndecan-1	422:466	the integral membrane proteoglycan Syndecan-1 (SDC1)	422:473	We elucidate a mechanism of soluble protein cargo sorting into secretory vesicles with a sphingomyelin-rich membrane; the integral membrane proteoglycan Syndecan-1 (SDC1) acts as a sorting receptor, capturing the soluble enzyme lipoprotein lipase (LPL) during export from the TGN.
31543446	3	4	theme	heparan	659:665	arg1	chains					675:680	SDC1-linked heparan sulfate chains	647:680	SDC1-linked heparan sulfate chains	647:680	Sorting of LPL requires bivalent interactions between LPL and SDC1-linked heparan sulfate chains and between LPL and the Golgi membrane.
31543446	1	5	theme	transport	162:170	arg1	carriers					172:179	vesicular transport carriers	152:179	vesicular transport carriers from the trans-Golgi network (TGN)	152:214	In the secretory pathway, budding of vesicular transport carriers from the trans-Golgi network (TGN) must coordinate specification of lipid composition with selection of secreted proteins.
31543446	1	6	theme	composition	255:265	arg1	specification					232:244	specification	232:244	specification of lipid composition	232:265	In the secretory pathway, budding of vesicular transport carriers from the trans-Golgi network (TGN) must coordinate specification of lipid composition with selection of secreted proteins.
31543446	2	7	theme	lipoprotein	532:542	arg1	LPL					552:554	LPL	552:554	LPL	552:554	We elucidate a mechanism of soluble protein cargo sorting into secretory vesicles with a sphingomyelin-rich membrane; the integral membrane proteoglycan Syndecan-1 (SDC1) acts as a sorting receptor, capturing the soluble enzyme lipoprotein lipase (LPL) during export from the TGN.
31543446	2	7	theme	lipoprotein	532:542	arg1	lipase					544:549	the soluble enzyme lipoprotein lipase	513:549	the soluble enzyme lipoprotein lipase (LPL)	513:555	We elucidate a mechanism of soluble protein cargo sorting into secretory vesicles with a sphingomyelin-rich membrane; the integral membrane proteoglycan Syndecan-1 (SDC1) acts as a sorting receptor, capturing the soluble enzyme lipoprotein lipase (LPL) during export from the TGN.
31543446	5	8	theme	biosynthetic	1133:1144	arg1	pathway					1156:1162	the biosynthetic secretory pathway	1129:1162	the biosynthetic secretory pathway	1129:1162	This study establishes that physicochemical properties of a protein transmembrane domain that drive lateral heterogeneity of the plasma membrane also operate at the TGN to confer sorting of an integral membrane protein and its ligand within the biosynthetic secretory pathway.
31543446	4	9	theme	secretion	869:877	arg1	pathway					879:885	the sphingomyelin secretion pathway	851:885	the sphingomyelin secretion pathway	851:885	Physical features of the SDC1 transmembrane domain, rather than a specific sequence, confer targeting of SDC1 and bound LPL into the sphingomyelin secretion pathway.
31543446	4	10	theme	domain	766:771	arg1	features					731:738	Physical features	722:738	Physical features of the SDC1 transmembrane domain, rather than a specific sequence,	722:805	Physical features of the SDC1 transmembrane domain, rather than a specific sequence, confer targeting of SDC1 and bound LPL into the sphingomyelin secretion pathway.
31543446	3	11	link	SDC1-linked	647:657	arg1	chains					675:680	SDC1-linked heparan sulfate chains	647:680	SDC1-linked heparan sulfate chains	647:680	Sorting of LPL requires bivalent interactions between LPL and SDC1-linked heparan sulfate chains and between LPL and the Golgi membrane.
31543446	2	12	theme	enzyme	525:530	arg1	LPL					552:554	LPL	552:554	LPL	552:554	We elucidate a mechanism of soluble protein cargo sorting into secretory vesicles with a sphingomyelin-rich membrane; the integral membrane proteoglycan Syndecan-1 (SDC1) acts as a sorting receptor, capturing the soluble enzyme lipoprotein lipase (LPL) during export from the TGN.
31543446	2	12	theme	enzyme	525:530	arg1	lipase					544:549	the soluble enzyme lipoprotein lipase	513:549	the soluble enzyme lipoprotein lipase (LPL)	513:555	We elucidate a mechanism of soluble protein cargo sorting into secretory vesicles with a sphingomyelin-rich membrane; the integral membrane proteoglycan Syndecan-1 (SDC1) acts as a sorting receptor, capturing the soluble enzyme lipoprotein lipase (LPL) during export from the TGN.
31543446	5	13	theme	membrane	1090:1097	arg1	protein					1099:1105	an integral membrane protein	1078:1105	an integral membrane protein	1078:1105	This study establishes that physicochemical properties of a protein transmembrane domain that drive lateral heterogeneity of the plasma membrane also operate at the TGN to confer sorting of an integral membrane protein and its ligand within the biosynthetic secretory pathway.
31543446	4	14	theme	sphingomyelin	855:867	arg1	pathway					879:885	the sphingomyelin secretion pathway	851:885	the sphingomyelin secretion pathway	851:885	Physical features of the SDC1 transmembrane domain, rather than a specific sequence, confer targeting of SDC1 and bound LPL into the sphingomyelin secretion pathway.
31543446	1	15	from	budding	141:147	arg1	TGN					211:213	TGN	211:213	TGN	211:213	In the secretory pathway, budding of vesicular transport carriers from the trans-Golgi network (TGN) must coordinate specification of lipid composition with selection of secreted proteins.
31543446	1	15	from	budding	141:147	arg1	network					202:208	the trans-Golgi network	186:208	the trans-Golgi network (TGN)	186:214	In the secretory pathway, budding of vesicular transport carriers from the trans-Golgi network (TGN) must coordinate specification of lipid composition with selection of secreted proteins.
31543446	4	16	theme	transmembrane	752:764	arg1	domain					766:771	the SDC1 transmembrane domain	743:771	the SDC1 transmembrane domain	743:771	Physical features of the SDC1 transmembrane domain, rather than a specific sequence, confer targeting of SDC1 and bound LPL into the sphingomyelin secretion pathway.
31543446	2	17	theme	soluble	517:523	arg1	LPL					552:554	LPL	552:554	LPL	552:554	We elucidate a mechanism of soluble protein cargo sorting into secretory vesicles with a sphingomyelin-rich membrane; the integral membrane proteoglycan Syndecan-1 (SDC1) acts as a sorting receptor, capturing the soluble enzyme lipoprotein lipase (LPL) during export from the TGN.
31543446	2	17	theme	soluble	517:523	arg1	lipase					544:549	the soluble enzyme lipoprotein lipase	513:549	the soluble enzyme lipoprotein lipase (LPL)	513:555	We elucidate a mechanism of soluble protein cargo sorting into secretory vesicles with a sphingomyelin-rich membrane; the integral membrane proteoglycan Syndecan-1 (SDC1) acts as a sorting receptor, capturing the soluble enzyme lipoprotein lipase (LPL) during export from the TGN.
31543446	5	18	theme	protein	1099:1105	arg1	ligand					1115:1120	its ligand	1111:1120	its ligand within the biosynthetic secretory pathway	1111:1162	This study establishes that physicochemical properties of a protein transmembrane domain that drive lateral heterogeneity of the plasma membrane also operate at the TGN to confer sorting of an integral membrane protein and its ligand within the biosynthetic secretory pathway.
31543446	5	18	theme	protein	1099:1105	arg1	sorting					1067:1073	sorting	1067:1073	sorting of an integral membrane protein	1067:1105	This study establishes that physicochemical properties of a protein transmembrane domain that drive lateral heterogeneity of the plasma membrane also operate at the TGN to confer sorting of an integral membrane protein and its ligand within the biosynthetic secretory pathway.
31543446	5	19	theme	physicochemical	916:930	arg1	properties					932:941	physicochemical properties	916:941	physicochemical properties of a protein transmembrane domain that drive lateral heterogeneity of the plasma membrane	916:1031	This study establishes that physicochemical properties of a protein transmembrane domain that drive lateral heterogeneity of the plasma membrane also operate at the TGN to confer sorting of an integral membrane protein and its ligand within the biosynthetic secretory pathway.
31543446	1	20	theme	carriers	172:179	arg1	budding					141:147	budding	141:147	budding of vesicular transport carriers from the trans-Golgi network (TGN)	141:214	In the secretory pathway, budding of vesicular transport carriers from the trans-Golgi network (TGN) must coordinate specification of lipid composition with selection of secreted proteins.
31543446	3	21	theme	Golgi	706:710	arg1	membrane					712:719	the Golgi membrane	702:719	the Golgi membrane	702:719	Sorting of LPL requires bivalent interactions between LPL and SDC1-linked heparan sulfate chains and between LPL and the Golgi membrane.
31543446	4	22	theme	SDC1	827:830	arg1	LPL					842:844	bound LPL	836:844	bound LPL into the sphingomyelin secretion pathway	836:885	Physical features of the SDC1 transmembrane domain, rather than a specific sequence, confer targeting of SDC1 and bound LPL into the sphingomyelin secretion pathway.
31543446	4	22	theme	SDC1	827:830	arg1	targeting					814:822	targeting	814:822	targeting of SDC1	814:830	Physical features of the SDC1 transmembrane domain, rather than a specific sequence, confer targeting of SDC1 and bound LPL into the sphingomyelin secretion pathway.
31543446	5	23	theme	lateral	988:994	arg1	heterogeneity					996:1008	lateral heterogeneity	988:1008	lateral heterogeneity of the plasma membrane	988:1031	This study establishes that physicochemical properties of a protein transmembrane domain that drive lateral heterogeneity of the plasma membrane also operate at the TGN to confer sorting of an integral membrane protein and its ligand within the biosynthetic secretory pathway.
31543446	2	24	theme	sphingomyelin-rich	393:410	arg1	membrane					412:419	a sphingomyelin-rich membrane	391:419	a sphingomyelin-rich membrane	391:419	We elucidate a mechanism of soluble protein cargo sorting into secretory vesicles with a sphingomyelin-rich membrane; the integral membrane proteoglycan Syndecan-1 (SDC1) acts as a sorting receptor, capturing the soluble enzyme lipoprotein lipase (LPL) during export from the TGN.
31543446	4	25	theme	bound	836:840	arg1	LPL					842:844	bound LPL	836:844	bound LPL into the sphingomyelin secretion pathway	836:885	Physical features of the SDC1 transmembrane domain, rather than a specific sequence, confer targeting of SDC1 and bound LPL into the sphingomyelin secretion pathway.
31543446	1	26	theme	trans-Golgi	190:200	arg1	TGN					211:213	TGN	211:213	TGN	211:213	In the secretory pathway, budding of vesicular transport carriers from the trans-Golgi network (TGN) must coordinate specification of lipid composition with selection of secreted proteins.
31543446	1	26	theme	trans-Golgi	190:200	arg1	network					202:208	the trans-Golgi network	186:208	the trans-Golgi network (TGN)	186:214	In the secretory pathway, budding of vesicular transport carriers from the trans-Golgi network (TGN) must coordinate specification of lipid composition with selection of secreted proteins.
31543446	1	27	theme	secreted	285:292	arg1	proteins					294:301	secreted proteins	285:301	secreted proteins	285:301	In the secretory pathway, budding of vesicular transport carriers from the trans-Golgi network (TGN) must coordinate specification of lipid composition with selection of secreted proteins.
31543446	4	28	theme	sequence	797:804	arg1	features					731:738	Physical features	722:738	Physical features of the SDC1 transmembrane domain, rather than a specific sequence,	722:805	Physical features of the SDC1 transmembrane domain, rather than a specific sequence, confer targeting of SDC1 and bound LPL into the sphingomyelin secretion pathway.
31543446	4	29	theme	SDC1	747:750	arg1	domain					766:771	the SDC1 transmembrane domain	743:771	the SDC1 transmembrane domain	743:771	Physical features of the SDC1 transmembrane domain, rather than a specific sequence, confer targeting of SDC1 and bound LPL into the sphingomyelin secretion pathway.
31543446	2	30	theme	membrane	435:442	arg1	SDC1					469:472	SDC1	469:472	SDC1	469:472	We elucidate a mechanism of soluble protein cargo sorting into secretory vesicles with a sphingomyelin-rich membrane; the integral membrane proteoglycan Syndecan-1 (SDC1) acts as a sorting receptor, capturing the soluble enzyme lipoprotein lipase (LPL) during export from the TGN.
31543446	2	30	theme	membrane	435:442	arg1	Syndecan-1					457:466	the integral membrane proteoglycan Syndecan-1	422:466	the integral membrane proteoglycan Syndecan-1 (SDC1)	422:473	We elucidate a mechanism of soluble protein cargo sorting into secretory vesicles with a sphingomyelin-rich membrane; the integral membrane proteoglycan Syndecan-1 (SDC1) acts as a sorting receptor, capturing the soluble enzyme lipoprotein lipase (LPL) during export from the TGN.
31543446	2	30	theme	membrane	435:442	arg1	receptor					493:500	a sorting receptor	483:500	a sorting receptor	483:500	We elucidate a mechanism of soluble protein cargo sorting into secretory vesicles with a sphingomyelin-rich membrane; the integral membrane proteoglycan Syndecan-1 (SDC1) acts as a sorting receptor, capturing the soluble enzyme lipoprotein lipase (LPL) during export from the TGN.
31543446	2	31	theme	proteoglycan	444:455	arg1	SDC1					469:472	SDC1	469:472	SDC1	469:472	We elucidate a mechanism of soluble protein cargo sorting into secretory vesicles with a sphingomyelin-rich membrane; the integral membrane proteoglycan Syndecan-1 (SDC1) acts as a sorting receptor, capturing the soluble enzyme lipoprotein lipase (LPL) during export from the TGN.
31543446	2	31	theme	proteoglycan	444:455	arg1	Syndecan-1					457:466	the integral membrane proteoglycan Syndecan-1	422:466	the integral membrane proteoglycan Syndecan-1 (SDC1)	422:473	We elucidate a mechanism of soluble protein cargo sorting into secretory vesicles with a sphingomyelin-rich membrane; the integral membrane proteoglycan Syndecan-1 (SDC1) acts as a sorting receptor, capturing the soluble enzyme lipoprotein lipase (LPL) during export from the TGN.
31543446	2	31	theme	proteoglycan	444:455	arg1	receptor					493:500	a sorting receptor	483:500	a sorting receptor	483:500	We elucidate a mechanism of soluble protein cargo sorting into secretory vesicles with a sphingomyelin-rich membrane; the integral membrane proteoglycan Syndecan-1 (SDC1) acts as a sorting receptor, capturing the soluble enzyme lipoprotein lipase (LPL) during export from the TGN.
31543446	1	32	theme	proteins	294:301	arg1	selection					272:280	selection	272:280	selection of secreted proteins	272:301	In the secretory pathway, budding of vesicular transport carriers from the trans-Golgi network (TGN) must coordinate specification of lipid composition with selection of secreted proteins.
31543446	2	33	theme	sorting	354:360	arg1	mechanism					319:327	a mechanism	317:327	a mechanism of soluble protein cargo sorting into secretory vesicles with a sphingomyelin-rich membrane	317:419	We elucidate a mechanism of soluble protein cargo sorting into secretory vesicles with a sphingomyelin-rich membrane; the integral membrane proteoglycan Syndecan-1 (SDC1) acts as a sorting receptor, capturing the soluble enzyme lipoprotein lipase (LPL) during export from the TGN.
31543446	2	34	theme	integral	426:433	arg1	SDC1					469:472	SDC1	469:472	SDC1	469:472	We elucidate a mechanism of soluble protein cargo sorting into secretory vesicles with a sphingomyelin-rich membrane; the integral membrane proteoglycan Syndecan-1 (SDC1) acts as a sorting receptor, capturing the soluble enzyme lipoprotein lipase (LPL) during export from the TGN.
31543446	2	34	theme	integral	426:433	arg1	Syndecan-1					457:466	the integral membrane proteoglycan Syndecan-1	422:466	the integral membrane proteoglycan Syndecan-1 (SDC1)	422:473	We elucidate a mechanism of soluble protein cargo sorting into secretory vesicles with a sphingomyelin-rich membrane; the integral membrane proteoglycan Syndecan-1 (SDC1) acts as a sorting receptor, capturing the soluble enzyme lipoprotein lipase (LPL) during export from the TGN.
31543446	2	34	theme	integral	426:433	arg1	receptor					493:500	a sorting receptor	483:500	a sorting receptor	483:500	We elucidate a mechanism of soluble protein cargo sorting into secretory vesicles with a sphingomyelin-rich membrane; the integral membrane proteoglycan Syndecan-1 (SDC1) acts as a sorting receptor, capturing the soluble enzyme lipoprotein lipase (LPL) during export from the TGN.
31543446	0	35	theme	Lipoprotein	39:49	arg1	Lipase					51:56	Soluble Lipoprotein Lipase	31:56	Soluble Lipoprotein Lipase with Sphingomyelin-Rich Membrane	31:89	Syndecan-1 Mediates Sorting of Soluble Lipoprotein Lipase with Sphingomyelin-Rich Membrane in the Golgi Apparatus.
31543446	2	36	theme	protein	340:346	arg1	sorting					354:360	soluble protein cargo sorting	332:360	soluble protein cargo sorting into secretory vesicles with a sphingomyelin-rich membrane	332:419	We elucidate a mechanism of soluble protein cargo sorting into secretory vesicles with a sphingomyelin-rich membrane; the integral membrane proteoglycan Syndecan-1 (SDC1) acts as a sorting receptor, capturing the soluble enzyme lipoprotein lipase (LPL) during export from the TGN.
31543446	3	37	theme	SDC1-linked	647:657	arg1	chains					675:680	SDC1-linked heparan sulfate chains	647:680	SDC1-linked heparan sulfate chains	647:680	Sorting of LPL requires bivalent interactions between LPL and SDC1-linked heparan sulfate chains and between LPL and the Golgi membrane.
31543446	5	38	theme	membrane	1024:1031	arg1	heterogeneity					996:1008	lateral heterogeneity	988:1008	lateral heterogeneity of the plasma membrane	988:1031	This study establishes that physicochemical properties of a protein transmembrane domain that drive lateral heterogeneity of the plasma membrane also operate at the TGN to confer sorting of an integral membrane protein and its ligand within the biosynthetic secretory pathway.
31543446	0	39	theme	Soluble	31:37	arg1	Lipase					51:56	Soluble Lipoprotein Lipase	31:56	Soluble Lipoprotein Lipase with Sphingomyelin-Rich Membrane	31:89	Syndecan-1 Mediates Sorting of Soluble Lipoprotein Lipase with Sphingomyelin-Rich Membrane in the Golgi Apparatus.
31543446	5	40	theme	secretory	1146:1154	arg1	pathway					1156:1162	the biosynthetic secretory pathway	1129:1162	the biosynthetic secretory pathway	1129:1162	This study establishes that physicochemical properties of a protein transmembrane domain that drive lateral heterogeneity of the plasma membrane also operate at the TGN to confer sorting of an integral membrane protein and its ligand within the biosynthetic secretory pathway.
31543446	5	41	gly	heterogeneity	996:1008	arg1	membrane					1024:1031	the plasma membrane	1013:1031	the plasma membrane	1013:1031	This study establishes that physicochemical properties of a protein transmembrane domain that drive lateral heterogeneity of the plasma membrane also operate at the TGN to confer sorting of an integral membrane protein and its ligand within the biosynthetic secretory pathway.
31543446	5	42	theme	integral	1081:1088	arg1	protein					1099:1105	an integral membrane protein	1078:1105	an integral membrane protein	1078:1105	This study establishes that physicochemical properties of a protein transmembrane domain that drive lateral heterogeneity of the plasma membrane also operate at the TGN to confer sorting of an integral membrane protein and its ligand within the biosynthetic secretory pathway.
31543446	2	43	from	TGN	580:582	arg1	export					564:569	export	564:569	export from the TGN	564:582	We elucidate a mechanism of soluble protein cargo sorting into secretory vesicles with a sphingomyelin-rich membrane; the integral membrane proteoglycan Syndecan-1 (SDC1) acts as a sorting receptor, capturing the soluble enzyme lipoprotein lipase (LPL) during export from the TGN.
31543446	2	44	theme	cargo	348:352	arg1	sorting					354:360	soluble protein cargo sorting	332:360	soluble protein cargo sorting into secretory vesicles with a sphingomyelin-rich membrane	332:419	We elucidate a mechanism of soluble protein cargo sorting into secretory vesicles with a sphingomyelin-rich membrane; the integral membrane proteoglycan Syndecan-1 (SDC1) acts as a sorting receptor, capturing the soluble enzyme lipoprotein lipase (LPL) during export from the TGN.
31543446	0	45	theme	Lipase	51:56	arg1	Sorting					20:26	Sorting	20:26	Sorting of Soluble Lipoprotein Lipase with Sphingomyelin-Rich Membrane in the Golgi Apparatus	20:112	Syndecan-1 Mediates Sorting of Soluble Lipoprotein Lipase with Sphingomyelin-Rich Membrane in the Golgi Apparatus.
31543446	1	46	from	network	202:208	arg1	carriers					172:179	vesicular transport carriers	152:179	vesicular transport carriers from the trans-Golgi network (TGN)	152:214	In the secretory pathway, budding of vesicular transport carriers from the trans-Golgi network (TGN) must coordinate specification of lipid composition with selection of secreted proteins.
31543446	1	46	from	network	202:208	arg1	budding					141:147	budding	141:147	budding of vesicular transport carriers from the trans-Golgi network (TGN)	141:214	In the secretory pathway, budding of vesicular transport carriers from the trans-Golgi network (TGN) must coordinate specification of lipid composition with selection of secreted proteins.
31543446	1	47	theme	secretory	122:130	arg1	pathway					132:138	the secretory pathway	118:138	the secretory pathway	118:138	In the secretory pathway, budding of vesicular transport carriers from the trans-Golgi network (TGN) must coordinate specification of lipid composition with selection of secreted proteins.
31543446	5	48	theme	plasma	1017:1022	arg1	membrane					1024:1031	the plasma membrane	1013:1031	the plasma membrane	1013:1031	This study establishes that physicochemical properties of a protein transmembrane domain that drive lateral heterogeneity of the plasma membrane also operate at the TGN to confer sorting of an integral membrane protein and its ligand within the biosynthetic secretory pathway.
31543446	3	49	theme	bivalent	609:616	arg1	interactions					618:629	bivalent interactions	609:629	bivalent interactions between LPL and SDC1-linked heparan sulfate chains and between LPL and the Golgi membrane	609:719	Sorting of LPL requires bivalent interactions between LPL and SDC1-linked heparan sulfate chains and between LPL and the Golgi membrane.
31543446	2	50	theme	soluble	332:338	arg1	sorting					354:360	soluble protein cargo sorting	332:360	soluble protein cargo sorting into secretory vesicles with a sphingomyelin-rich membrane	332:419	We elucidate a mechanism of soluble protein cargo sorting into secretory vesicles with a sphingomyelin-rich membrane; the integral membrane proteoglycan Syndecan-1 (SDC1) acts as a sorting receptor, capturing the soluble enzyme lipoprotein lipase (LPL) during export from the TGN.
31543446	5	51	theme	protein	948:954	arg1	domain					970:975	a protein transmembrane domain	946:975	a protein transmembrane domain that drive lateral heterogeneity of the plasma membrane	946:1031	This study establishes that physicochemical properties of a protein transmembrane domain that drive lateral heterogeneity of the plasma membrane also operate at the TGN to confer sorting of an integral membrane protein and its ligand within the biosynthetic secretory pathway.
31543446	2	52	with	vesicles	377:384	arg1	membrane					412:419	a sphingomyelin-rich membrane	391:419	a sphingomyelin-rich membrane	391:419	We elucidate a mechanism of soluble protein cargo sorting into secretory vesicles with a sphingomyelin-rich membrane; the integral membrane proteoglycan Syndecan-1 (SDC1) acts as a sorting receptor, capturing the soluble enzyme lipoprotein lipase (LPL) during export from the TGN.
31543446	5	53	theme	domain	970:975	arg1	properties					932:941	physicochemical properties	916:941	physicochemical properties of a protein transmembrane domain that drive lateral heterogeneity of the plasma membrane	916:1031	This study establishes that physicochemical properties of a protein transmembrane domain that drive lateral heterogeneity of the plasma membrane also operate at the TGN to confer sorting of an integral membrane protein and its ligand within the biosynthetic secretory pathway.
31543446	4	54	theme	Physical	722:729	arg1	features					731:738	Physical features	722:738	Physical features of the SDC1 transmembrane domain, rather than a specific sequence,	722:805	Physical features of the SDC1 transmembrane domain, rather than a specific sequence, confer targeting of SDC1 and bound LPL into the sphingomyelin secretion pathway.
31543446	3	55	theme	sulfate	667:673	arg1	chains					675:680	SDC1-linked heparan sulfate chains	647:680	SDC1-linked heparan sulfate chains	647:680	Sorting of LPL requires bivalent interactions between LPL and SDC1-linked heparan sulfate chains and between LPL and the Golgi membrane.
31543446	3	56	theme	LPL	596:598	arg1	Sorting					585:591	Sorting	585:591	Sorting of LPL	585:598	Sorting of LPL requires bivalent interactions between LPL and SDC1-linked heparan sulfate chains and between LPL and the Golgi membrane.
31543446	0	57	with	Lipase	51:56	arg1	Membrane					82:89	Sphingomyelin-Rich Membrane	63:89	Sphingomyelin-Rich Membrane	63:89	Syndecan-1 Mediates Sorting of Soluble Lipoprotein Lipase with Sphingomyelin-Rich Membrane in the Golgi Apparatus.
31543446	5	58	theme	transmembrane	956:968	arg1	domain					970:975	a protein transmembrane domain	946:975	a protein transmembrane domain that drive lateral heterogeneity of the plasma membrane	946:1031	This study establishes that physicochemical properties of a protein transmembrane domain that drive lateral heterogeneity of the plasma membrane also operate at the TGN to confer sorting of an integral membrane protein and its ligand within the biosynthetic secretory pathway.
31543446	0	59	theme	Sphingomyelin-Rich	63:80	arg1	Membrane					82:89	Sphingomyelin-Rich Membrane	63:89	Sphingomyelin-Rich Membrane	63:89	Syndecan-1 Mediates Sorting of Soluble Lipoprotein Lipase with Sphingomyelin-Rich Membrane in the Golgi Apparatus.
31543446	4	60	theme	specific	788:795	arg1	sequence					797:804	a specific sequence	786:804	a specific sequence	786:804	Physical features of the SDC1 transmembrane domain, rather than a specific sequence, confer targeting of SDC1 and bound LPL into the sphingomyelin secretion pathway.
31543446	2	61	theme	secretory	367:375	arg1	vesicles					377:384	secretory vesicles	367:384	secretory vesicles with a sphingomyelin-rich membrane	367:419	We elucidate a mechanism of soluble protein cargo sorting into secretory vesicles with a sphingomyelin-rich membrane; the integral membrane proteoglycan Syndecan-1 (SDC1) acts as a sorting receptor, capturing the soluble enzyme lipoprotein lipase (LPL) during export from the TGN.
31543446	0	62	theme	Golgi	98:102	arg1	Apparatus					104:112	the Golgi Apparatus	94:112	the Golgi Apparatus	94:112	Syndecan-1 Mediates Sorting of Soluble Lipoprotein Lipase with Sphingomyelin-Rich Membrane in the Golgi Apparatus.
30739725	8	0	theme	glycan	1100:1105	arg1	structure					1107:1115	the pilin glycan structure	1090:1115	the pilin glycan structure	1090:1115	Moreover, by deleting genes responsible for the pilin glycan structure, we show that the number of saccharides attached at residue serine 63 affects the ratio between surface tension and viscosity and cause sorting between bacteria carrying different pilin glycoforms.
30739725	7	1	theme	increased	986:994	arg1	forces					1007:1012	increased attractive forces	986:1012	increased attractive forces generated by T4P between cells	986:1043	We relate these mesoscopic phenotypes to increased attractive forces generated by T4P between cells.
30739725	0	2	theme	Colonies	89:96	arg1	Properties					65:74	Material Properties	56:74	Material Properties of Bacterial Colonies	56:96	Type IV Pilin Post-Translational Modifications Modulate Material Properties of Bacterial Colonies.
30739725	4	3	theme	multiple	386:393	arg1	modifications					414:426	multiple post-translational modifications	386:426	multiple post-translational modifications	386:426	The major pilin subunit of the T4P bears multiple post-translational modifications.
30739725	9	4	theme	post-translational	1348:1365	arg1	modifications					1367:1379	different pilin post-translational modifications	1332:1379	different pilin post-translational modifications	1332:1379	We conclude that different pilin post-translational modifications moderately affect the attractive forces between bacteria but have severe effects on the material properties of microcolonies.
30739725	7	5	theme	mesoscopic	961:970	arg1	phenotypes					972:981	these mesoscopic phenotypes	955:981	these mesoscopic phenotypes	955:981	We relate these mesoscopic phenotypes to increased attractive forces generated by T4P between cells.
30739725	6	6	contain	carrying	860:867	arg2	modification					885:896	the phosphoform modification	869:896	the phosphoform modification	869:896	Deleting the phosphotransferase responsible for phosphoethanolamine modification at residue serine 68 inhibits shape relaxations of microcolonies after perturbation and causes bacteria carrying the phosphoform modification to segregate to the surface of mixed colonies.
30739725	6	6	contain	carrying	860:867	arg1	bacteria					851:858	bacteria	851:858	bacteria carrying the phosphoform modification	851:896	Deleting the phosphotransferase responsible for phosphoethanolamine modification at residue serine 68 inhibits shape relaxations of microcolonies after perturbation and causes bacteria carrying the phosphoform modification to segregate to the surface of mixed colonies.
30739725	8	7	theme	pilin	1094:1098	arg1	structure					1107:1115	the pilin glycan structure	1090:1115	the pilin glycan structure	1090:1115	Moreover, by deleting genes responsible for the pilin glycan structure, we show that the number of saccharides attached at residue serine 63 affects the ratio between surface tension and viscosity and cause sorting between bacteria carrying different pilin glycoforms.
30739725	5	8	theme	microcolonies	628:640	arg1	dynamics					616:623	dynamics	616:623	dynamics	616:623	By interfering with different steps of the pilin glycosylation and phosphoform modification pathways, we investigated the effect of pilin post-translational modification on the shape and dynamics of microcolonies formed by Neisseria gonorrhoeae.
30739725	5	8	theme	microcolonies	628:640	arg1	shape					606:610	shape	606:610	shape	606:610	By interfering with different steps of the pilin glycosylation and phosphoform modification pathways, we investigated the effect of pilin post-translational modification on the shape and dynamics of microcolonies formed by Neisseria gonorrhoeae.
30739725	5	9	dep	shape	606:610	arg1	the					602:604	the	602:604	the	602:604	By interfering with different steps of the pilin glycosylation and phosphoform modification pathways, we investigated the effect of pilin post-translational modification on the shape and dynamics of microcolonies formed by Neisseria gonorrhoeae.
30739725	6	10	from	serine	767:772	arg1	modification					743:754	phosphoethanolamine modification	723:754	phosphoethanolamine modification at residue serine 68	723:775	Deleting the phosphotransferase responsible for phosphoethanolamine modification at residue serine 68 inhibits shape relaxations of microcolonies after perturbation and causes bacteria carrying the phosphoform modification to segregate to the surface of mixed colonies.
30739725	5	11	theme	modification	508:519	arg1	pathways					521:528	the pilin glycosylation and phosphoform modification pathways	468:528	pathways	521:528	By interfering with different steps of the pilin glycosylation and phosphoform modification pathways, we investigated the effect of pilin post-translational modification on the shape and dynamics of microcolonies formed by Neisseria gonorrhoeae.
30739725	6	12	theme	shape	786:790	arg1	relaxations					792:802	shape relaxations	786:802	shape relaxations of microcolonies	786:819	Deleting the phosphotransferase responsible for phosphoethanolamine modification at residue serine 68 inhibits shape relaxations of microcolonies after perturbation and causes bacteria carrying the phosphoform modification to segregate to the surface of mixed colonies.
30739725	5	13	theme	Neisseria	652:660	arg1	gonorrhoeae					662:672	Neisseria gonorrhoeae	652:672	Neisseria gonorrhoeae	652:672	By interfering with different steps of the pilin glycosylation and phosphoform modification pathways, we investigated the effect of pilin post-translational modification on the shape and dynamics of microcolonies formed by Neisseria gonorrhoeae.
30739725	4	14	theme	post-translational	395:412	arg1	modifications					414:426	multiple post-translational modifications	386:426	multiple post-translational modifications	386:426	The major pilin subunit of the T4P bears multiple post-translational modifications.
30739725	5	15	theme	pathways	521:528	arg1	steps					459:463	different steps	449:463	different steps of the pilin glycosylation and phosphoform modification pathways	449:528	By interfering with different steps of the pilin glycosylation and phosphoform modification pathways, we investigated the effect of pilin post-translational modification on the shape and dynamics of microcolonies formed by Neisseria gonorrhoeae.
30739725	5	16	from	effect	551:556	arg1	dynamics					616:623	dynamics	616:623	dynamics	616:623	By interfering with different steps of the pilin glycosylation and phosphoform modification pathways, we investigated the effect of pilin post-translational modification on the shape and dynamics of microcolonies formed by Neisseria gonorrhoeae.
30739725	5	16	from	effect	551:556	arg1	shape					606:610	shape	606:610	shape	606:610	By interfering with different steps of the pilin glycosylation and phosphoform modification pathways, we investigated the effect of pilin post-translational modification on the shape and dynamics of microcolonies formed by Neisseria gonorrhoeae.
30739725	9	17	theme	severe	1447:1452	arg1	effects					1454:1460	severe effects	1447:1460	severe effects on the material properties of microcolonies	1447:1504	We conclude that different pilin post-translational modifications moderately affect the attractive forces between bacteria but have severe effects on the material properties of microcolonies.
30739725	8	18	gly	glycoforms	1303:1312	arg1	pilin					1297:1301	different pilin glycoforms	1287:1312	different pilin glycoforms	1287:1312	Moreover, by deleting genes responsible for the pilin glycan structure, we show that the number of saccharides attached at residue serine 63 affects the ratio between surface tension and viscosity and cause sorting between bacteria carrying different pilin glycoforms.
30739725	9	19	from	effects	1454:1460	arg1	properties					1478:1487	the material properties	1465:1487	the material properties of microcolonies	1465:1504	We conclude that different pilin post-translational modifications moderately affect the attractive forces between bacteria but have severe effects on the material properties of microcolonies.
30739725	0	20	theme	IV	5:6	arg1	Modifications					33:45	Type IV Pilin Post-Translational Modifications	0:45	Type IV Pilin Post-Translational Modifications	0:45	Type IV Pilin Post-Translational Modifications Modulate Material Properties of Bacterial Colonies.
30739725	6	21	theme	phosphoform	873:883	arg1	modification					885:896	the phosphoform modification	869:896	the phosphoform modification	869:896	Deleting the phosphotransferase responsible for phosphoethanolamine modification at residue serine 68 inhibits shape relaxations of microcolonies after perturbation and causes bacteria carrying the phosphoform modification to segregate to the surface of mixed colonies.
30739725	1	22	theme	Bacterial	99:107	arg1	polymers					145:152	extracellular polymers	131:152	extracellular polymers that initiate the formation of microcolonies and biofilms	131:210	Bacterial type 4 pili (T4P) are extracellular polymers that initiate the formation of microcolonies and biofilms.
30739725	1	22	theme	Bacterial	99:107	arg1	T4P					122:124	T4P	122:124	T4P	122:124	Bacterial type 4 pili (T4P) are extracellular polymers that initiate the formation of microcolonies and biofilms.
30739725	1	22	theme	Bacterial	99:107	arg1	pili					116:119	Bacterial type 4 pili	99:119	Bacterial type 4 pili (T4P)	99:125	Bacterial type 4 pili (T4P) are extracellular polymers that initiate the formation of microcolonies and biofilms.
30739725	0	23	theme	Type	0:3	arg1	Modifications					33:45	Type IV Pilin Post-Translational Modifications	0:45	Type IV Pilin Post-Translational Modifications	0:45	Type IV Pilin Post-Translational Modifications Modulate Material Properties of Bacterial Colonies.
30739725	8	24	theme	saccharides	1145:1155	arg1	number					1135:1140	the number	1131:1140	the number of saccharides attached at residue serine 63	1131:1185	Moreover, by deleting genes responsible for the pilin glycan structure, we show that the number of saccharides attached at residue serine 63 affects the ratio between surface tension and viscosity and cause sorting between bacteria carrying different pilin glycoforms.
30739725	4	25	theme	T4P	376:378	arg1	subunit					361:367	The major pilin subunit	345:367	The major pilin subunit of the T4P	345:378	The major pilin subunit of the T4P bears multiple post-translational modifications.
30739725	1	26	theme	type	109:112	arg1	polymers					145:152	extracellular polymers	131:152	extracellular polymers that initiate the formation of microcolonies and biofilms	131:210	Bacterial type 4 pili (T4P) are extracellular polymers that initiate the formation of microcolonies and biofilms.
30739725	1	26	theme	type	109:112	arg1	T4P					122:124	T4P	122:124	T4P	122:124	Bacterial type 4 pili (T4P) are extracellular polymers that initiate the formation of microcolonies and biofilms.
30739725	1	26	theme	type	109:112	arg1	pili					116:119	Bacterial type 4 pili	99:119	Bacterial type 4 pili (T4P)	99:125	Bacterial type 4 pili (T4P) are extracellular polymers that initiate the formation of microcolonies and biofilms.
30739725	5	27	theme	pilin	472:476	arg1	glycosylation					478:490	the pilin glycosylation and phosphoform modification pathways	468:528	glycosylation	478:490	By interfering with different steps of the pilin glycosylation and phosphoform modification pathways, we investigated the effect of pilin post-translational modification on the shape and dynamics of microcolonies formed by Neisseria gonorrhoeae.
30739725	1	28	theme	microcolonies	185:197	arg1	formation					172:180	the formation	168:180	the formation of microcolonies and biofilms	168:210	Bacterial type 4 pili (T4P) are extracellular polymers that initiate the formation of microcolonies and biofilms.
30739725	0	29	theme	Post-Translational	14:31	arg1	Modifications					33:45	Type IV Pilin Post-Translational Modifications	0:45	Type IV Pilin Post-Translational Modifications	0:45	Type IV Pilin Post-Translational Modifications Modulate Material Properties of Bacterial Colonies.
30739725	8	30	dep	viscosity	1233:1241	arg1	sorting					1253:1259	sorting	1253:1259	sorting	1253:1259	Moreover, by deleting genes responsible for the pilin glycan structure, we show that the number of saccharides attached at residue serine 63 affects the ratio between surface tension and viscosity and cause sorting between bacteria carrying different pilin glycoforms.
30739725	8	31	theme	residue	1169:1175	arg1	serine					1177:1182	residue serine 63	1169:1185	residue serine 63	1169:1185	Moreover, by deleting genes responsible for the pilin glycan structure, we show that the number of saccharides attached at residue serine 63 affects the ratio between surface tension and viscosity and cause sorting between bacteria carrying different pilin glycoforms.
30739725	5	32	theme	glycosylation	478:490	arg1	steps					459:463	different steps	449:463	different steps of the pilin glycosylation and phosphoform modification pathways	449:528	By interfering with different steps of the pilin glycosylation and phosphoform modification pathways, we investigated the effect of pilin post-translational modification on the shape and dynamics of microcolonies formed by Neisseria gonorrhoeae.
30739725	9	33	theme	different	1332:1340	arg1	modifications					1367:1379	different pilin post-translational modifications	1332:1379	different pilin post-translational modifications	1332:1379	We conclude that different pilin post-translational modifications moderately affect the attractive forces between bacteria but have severe effects on the material properties of microcolonies.
30739725	0	34	theme	Pilin	8:12	arg1	Modifications					33:45	Type IV Pilin Post-Translational Modifications	0:45	Type IV Pilin Post-Translational Modifications	0:45	Type IV Pilin Post-Translational Modifications Modulate Material Properties of Bacterial Colonies.
30739725	9	35	theme	material	1469:1476	arg1	properties					1478:1487	the material properties	1465:1487	the material properties of microcolonies	1465:1504	We conclude that different pilin post-translational modifications moderately affect the attractive forces between bacteria but have severe effects on the material properties of microcolonies.
30739725	3	36	theme	local	294:298	arg1	order					300:304	the local order	290:304	the local order	290:304	These pilus dynamics crucially affect the local order, shape, and fluidity of microcolonies.
30739725	6	37	theme	phosphoethanolamine	723:741	arg1	modification					743:754	phosphoethanolamine modification	723:754	phosphoethanolamine modification at residue serine 68	723:775	Deleting the phosphotransferase responsible for phosphoethanolamine modification at residue serine 68 inhibits shape relaxations of microcolonies after perturbation and causes bacteria carrying the phosphoform modification to segregate to the surface of mixed colonies.
30739725	9	38	contain	have	1442:1445	arg2	effects					1454:1460	severe effects	1447:1460	severe effects on the material properties of microcolonies	1447:1504	We conclude that different pilin post-translational modifications moderately affect the attractive forces between bacteria but have severe effects on the material properties of microcolonies.
30739725	9	38	contain	have	1442:1445	arg1	modifications					1367:1379	different pilin post-translational modifications	1332:1379	different pilin post-translational modifications	1332:1379	We conclude that different pilin post-translational modifications moderately affect the attractive forces between bacteria but have severe effects on the material properties of microcolonies.
30739725	1	39	theme	biofilms	203:210	arg1	formation					172:180	the formation	168:180	the formation of microcolonies and biofilms	168:210	Bacterial type 4 pili (T4P) are extracellular polymers that initiate the formation of microcolonies and biofilms.
30739725	8	40	theme	responsible	1074:1084	arg1	genes					1068:1072	genes	1068:1072	genes responsible for the pilin glycan structure	1068:1115	Moreover, by deleting genes responsible for the pilin glycan structure, we show that the number of saccharides attached at residue serine 63 affects the ratio between surface tension and viscosity and cause sorting between bacteria carrying different pilin glycoforms.
30739725	5	41	theme	modification	586:597	arg1	effect					551:556	the effect	547:556	the effect of pilin post-translational modification on the shape and dynamics of microcolonies formed by Neisseria gonorrhoeae	547:672	By interfering with different steps of the pilin glycosylation and phosphoform modification pathways, we investigated the effect of pilin post-translational modification on the shape and dynamics of microcolonies formed by Neisseria gonorrhoeae.
30739725	5	42	theme	phosphoform	496:506	arg1	pathways					521:528	the pilin glycosylation and phosphoform modification pathways	468:528	pathways	521:528	By interfering with different steps of the pilin glycosylation and phosphoform modification pathways, we investigated the effect of pilin post-translational modification on the shape and dynamics of microcolonies formed by Neisseria gonorrhoeae.
30739725	6	43	theme	residue	759:765	arg1	serine					767:772	residue serine 68	759:775	residue serine 68	759:775	Deleting the phosphotransferase responsible for phosphoethanolamine modification at residue serine 68 inhibits shape relaxations of microcolonies after perturbation and causes bacteria carrying the phosphoform modification to segregate to the surface of mixed colonies.
30739725	8	44	theme	pilin	1297:1301	arg1	glycoforms					1303:1312	different pilin glycoforms	1287:1312	different pilin glycoforms	1287:1312	Moreover, by deleting genes responsible for the pilin glycan structure, we show that the number of saccharides attached at residue serine 63 affects the ratio between surface tension and viscosity and cause sorting between bacteria carrying different pilin glycoforms.
30739725	5	45	theme	pilin	561:565	arg1	modification					586:597	pilin post-translational modification	561:597	pilin post-translational modification	561:597	By interfering with different steps of the pilin glycosylation and phosphoform modification pathways, we investigated the effect of pilin post-translational modification on the shape and dynamics of microcolonies formed by Neisseria gonorrhoeae.
30739725	8	46	attach	attached	1157:1164	arg3	serine					1177:1182	residue serine 63	1169:1185	residue serine 63	1169:1185	Moreover, by deleting genes responsible for the pilin glycan structure, we show that the number of saccharides attached at residue serine 63 affects the ratio between surface tension and viscosity and cause sorting between bacteria carrying different pilin glycoforms.
30739725	8	46	attach	attached	1157:1164	arg2	saccharides					1145:1155	saccharides	1145:1155	saccharides attached at residue serine 63	1145:1185	Moreover, by deleting genes responsible for the pilin glycan structure, we show that the number of saccharides attached at residue serine 63 affects the ratio between surface tension and viscosity and cause sorting between bacteria carrying different pilin glycoforms.
30739725	4	47	contain	bears	380:384	arg2	modifications					414:426	multiple post-translational modifications	386:426	multiple post-translational modifications	386:426	The major pilin subunit of the T4P bears multiple post-translational modifications.
30739725	4	47	contain	bears	380:384	arg1	subunit					361:367	The major pilin subunit	345:367	The major pilin subunit of the T4P	345:378	The major pilin subunit of the T4P bears multiple post-translational modifications.
30739725	9	48	theme	microcolonies	1492:1504	arg1	properties					1478:1487	the material properties	1465:1487	the material properties of microcolonies	1465:1504	We conclude that different pilin post-translational modifications moderately affect the attractive forces between bacteria but have severe effects on the material properties of microcolonies.
30739725	8	49	theme	surface	1213:1219	arg1	tension					1221:1227	surface tension	1213:1227	surface tension	1213:1227	Moreover, by deleting genes responsible for the pilin glycan structure, we show that the number of saccharides attached at residue serine 63 affects the ratio between surface tension and viscosity and cause sorting between bacteria carrying different pilin glycoforms.
30739725	5	50	theme	post-translational	567:584	arg1	modification					586:597	pilin post-translational modification	561:597	pilin post-translational modification	561:597	By interfering with different steps of the pilin glycosylation and phosphoform modification pathways, we investigated the effect of pilin post-translational modification on the shape and dynamics of microcolonies formed by Neisseria gonorrhoeae.
30739725	0	51	theme	Material	56:63	arg1	Properties					65:74	Material Properties	56:74	Material Properties of Bacterial Colonies	56:96	Type IV Pilin Post-Translational Modifications Modulate Material Properties of Bacterial Colonies.
30739725	6	52	theme	colonies	935:942	arg1	surface					918:924	the surface	914:924	the surface of mixed colonies	914:942	Deleting the phosphotransferase responsible for phosphoethanolamine modification at residue serine 68 inhibits shape relaxations of microcolonies after perturbation and causes bacteria carrying the phosphoform modification to segregate to the surface of mixed colonies.
30739725	4	53	theme	pilin	355:359	arg1	subunit					361:367	The major pilin subunit	345:367	The major pilin subunit of the T4P	345:378	The major pilin subunit of the T4P bears multiple post-translational modifications.
30739725	9	54	theme	pilin	1342:1346	arg1	modifications					1367:1379	different pilin post-translational modifications	1332:1379	different pilin post-translational modifications	1332:1379	We conclude that different pilin post-translational modifications moderately affect the attractive forces between bacteria but have severe effects on the material properties of microcolonies.
30739725	5	55	theme	different	449:457	arg1	steps					459:463	different steps	449:463	different steps of the pilin glycosylation and phosphoform modification pathways	449:528	By interfering with different steps of the pilin glycosylation and phosphoform modification pathways, we investigated the effect of pilin post-translational modification on the shape and dynamics of microcolonies formed by Neisseria gonorrhoeae.
30739725	6	56	theme	mixed	929:933	arg1	colonies					935:942	mixed colonies	929:942	mixed colonies	929:942	Deleting the phosphotransferase responsible for phosphoethanolamine modification at residue serine 68 inhibits shape relaxations of microcolonies after perturbation and causes bacteria carrying the phosphoform modification to segregate to the surface of mixed colonies.
30739725	4	57	theme	major	349:353	arg1	subunit					361:367	The major pilin subunit	345:367	The major pilin subunit of the T4P	345:378	The major pilin subunit of the T4P bears multiple post-translational modifications.
30739725	6	58	theme	responsible	707:717	arg1	phosphotransferase					688:705	the phosphotransferase	684:705	the phosphotransferase responsible for phosphoethanolamine modification at residue serine 68	684:775	Deleting the phosphotransferase responsible for phosphoethanolamine modification at residue serine 68 inhibits shape relaxations of microcolonies after perturbation and causes bacteria carrying the phosphoform modification to segregate to the surface of mixed colonies.
30739725	3	59	theme	pilus	258:262	arg1	dynamics					264:271	These pilus dynamics	252:271	These pilus dynamics	252:271	These pilus dynamics crucially affect the local order, shape, and fluidity of microcolonies.
30739725	7	60	theme	attractive	996:1005	arg1	forces					1007:1012	increased attractive forces	986:1012	increased attractive forces generated by T4P between cells	986:1043	We relate these mesoscopic phenotypes to increased attractive forces generated by T4P between cells.
30739725	0	61	theme	Bacterial	79:87	arg1	Colonies					89:96	Bacterial Colonies	79:96	Bacterial Colonies	79:96	Type IV Pilin Post-Translational Modifications Modulate Material Properties of Bacterial Colonies.
30739725	6	62	theme	microcolonies	807:819	arg1	relaxations					792:802	shape relaxations	786:802	shape relaxations of microcolonies	786:819	Deleting the phosphotransferase responsible for phosphoethanolamine modification at residue serine 68 inhibits shape relaxations of microcolonies after perturbation and causes bacteria carrying the phosphoform modification to segregate to the surface of mixed colonies.
30739725	1	63	theme	extracellular	131:143	arg1	polymers					145:152	extracellular polymers	131:152	extracellular polymers that initiate the formation of microcolonies and biofilms	131:210	Bacterial type 4 pili (T4P) are extracellular polymers that initiate the formation of microcolonies and biofilms.
30739725	1	63	theme	extracellular	131:143	arg1	pili					116:119	Bacterial type 4 pili	99:119	Bacterial type 4 pili (T4P)	99:125	Bacterial type 4 pili (T4P) are extracellular polymers that initiate the formation of microcolonies and biofilms.
30739725	8	64	theme	different	1287:1295	arg1	glycoforms					1303:1312	different pilin glycoforms	1287:1312	different pilin glycoforms	1287:1312	Moreover, by deleting genes responsible for the pilin glycan structure, we show that the number of saccharides attached at residue serine 63 affects the ratio between surface tension and viscosity and cause sorting between bacteria carrying different pilin glycoforms.
30739725	3	65	theme	microcolonies	330:342	arg1	shape					307:311	shape	307:311	shape	307:311	These pilus dynamics crucially affect the local order, shape, and fluidity of microcolonies.
30739725	3	65	theme	microcolonies	330:342	arg1	fluidity					318:325	fluidity	318:325	fluidity	318:325	These pilus dynamics crucially affect the local order, shape, and fluidity of microcolonies.
30739725	3	65	theme	microcolonies	330:342	arg1	order					300:304	the local order	290:304	the local order	290:304	These pilus dynamics crucially affect the local order, shape, and fluidity of microcolonies.
30739725	9	66	theme	attractive	1403:1412	arg1	forces					1414:1419	the attractive forces	1399:1419	the attractive forces between bacteria	1399:1436	We conclude that different pilin post-translational modifications moderately affect the attractive forces between bacteria but have severe effects on the material properties of microcolonies.
30739725	8	67	contain	carrying	1278:1285	arg1	bacteria					1269:1276	bacteria	1269:1276	bacteria carrying different pilin glycoforms	1269:1312	Moreover, by deleting genes responsible for the pilin glycan structure, we show that the number of saccharides attached at residue serine 63 affects the ratio between surface tension and viscosity and cause sorting between bacteria carrying different pilin glycoforms.
30739725	8	67	contain	carrying	1278:1285	arg2	glycoforms					1303:1312	different pilin glycoforms	1287:1312	different pilin glycoforms	1287:1312	Moreover, by deleting genes responsible for the pilin glycan structure, we show that the number of saccharides attached at residue serine 63 affects the ratio between surface tension and viscosity and cause sorting between bacteria carrying different pilin glycoforms.
30999851	6	0	theme	material	895:902	arg1	analyses					869:876	preliminary chemical and structural analyses	833:876	preliminary chemical and structural analyses of extracellular material that seals the graft union	833:929	So, this study also aimed to describe the histological and cellular changes that accompany autografting of Arabidopsis hypocotyls and to perform preliminary chemical and structural analyses of extracellular material that seals the graft union.
30999851	5	1	theme	layer	625:629	arg1	formation					610:618	the formation	606:618	the formation of a layer that covers the surface of the graft union	606:672	During the study, the formation of a layer that covers the surface of the graft union was observed.
30999851	11	2	theme	union	1930:1934	arg1	surface					1913:1919	the surface	1909:1919	the surface of graft union	1909:1934	CONCLUSIONS To the best of our knowledge, this is the first report on the composition and structure of the extracellular material that gets deposited on the surface of graft union during Arabidopsis grafting.
30999851	9	3	theme	open	1419:1422	arg1	"					1423:1423	"open"	1418:1423	"open"	1418:1423	These bead-like structures appeared either "closed" or "open".
30999851	6	4	theme	structural	858:867	arg1	analyses					869:876	preliminary chemical and structural analyses	833:876	preliminary chemical and structural analyses of extracellular material that seals the graft union	833:929	So, this study also aimed to describe the histological and cellular changes that accompany autografting of Arabidopsis hypocotyls and to perform preliminary chemical and structural analyses of extracellular material that seals the graft union.
30999851	8	5	theme	network	1196:1202	arg1	formation					1171:1179	the formation	1167:1179	the formation of a fibrillar network	1167:1202	The spatiotemporal changes observed in the structure of the extracellular material included the formation of a fibrillar network, polymerization of the fibrillar network into a membranous layer, and the presence of bead-like structures on the surface of cells in established graft union.
30999851	8	5	theme	network	1196:1202	arg1	polymerization					1205:1218	polymerization	1205:1218	polymerization of the fibrillar network into a membranous layer	1205:1267	The spatiotemporal changes observed in the structure of the extracellular material included the formation of a fibrillar network, polymerization of the fibrillar network into a membranous layer, and the presence of bead-like structures on the surface of cells in established graft union.
30999851	8	5	theme	network	1196:1202	arg1	presence					1278:1285	the presence	1274:1285	the presence of bead-like structures on the surface of cells in established graft union	1274:1360	The spatiotemporal changes observed in the structure of the extracellular material included the formation of a fibrillar network, polymerization of the fibrillar network into a membranous layer, and the presence of bead-like structures on the surface of cells in established graft union.
30999851	6	6	theme	chemical	845:852	arg1	analyses					869:876	preliminary chemical and structural analyses	833:876	preliminary chemical and structural analyses of extracellular material that seals the graft union	833:929	So, this study also aimed to describe the histological and cellular changes that accompany autografting of Arabidopsis hypocotyls and to perform preliminary chemical and structural analyses of extracellular material that seals the graft union.
30999851	12	7	theme	scion	2081:2085	arg1	adhesion					2069:2076	the adhesion	2065:2076	the adhesion of scion and stock, as well as taking part in sealing the graft union	2065:2146	The results showed that unmethyl-esterified homogalacturonan and extensins are together involved in the adhesion of scion and stock, as well as taking part in sealing the graft union.
30999851	14	8	theme	composition	2393:2403	arg1	analysis					2377:2384	a detailed analysis	2366:2384	a detailed analysis of the composition and structure of the extracellular material	2366:2447	The findings presented here implicate a need for studies with biochemical approach for a detailed analysis of the composition and structure of the extracellular material.
30999851	8	9	attach	presence	1278:1285	arg2	network					1237:1243	the fibrillar network	1223:1243	the fibrillar network	1223:1243	The spatiotemporal changes observed in the structure of the extracellular material included the formation of a fibrillar network, polymerization of the fibrillar network into a membranous layer, and the presence of bead-like structures on the surface of cells in established graft union.
30999851	8	9	attach	presence	1278:1285	arg1	surface					1318:1324	the surface	1314:1324	the surface of cells in established graft union	1314:1360	The spatiotemporal changes observed in the structure of the extracellular material included the formation of a fibrillar network, polymerization of the fibrillar network into a membranous layer, and the presence of bead-like structures on the surface of cells in established graft union.
30999851	8	9	attach	presence	1278:1285	arg2	network					1196:1202	a fibrillar network	1184:1202	a fibrillar network	1184:1202	The spatiotemporal changes observed in the structure of the extracellular material included the formation of a fibrillar network, polymerization of the fibrillar network into a membranous layer, and the presence of bead-like structures on the surface of cells in established graft union.
30999851	8	9	attach	presence	1278:1285	arg2	structures					1300:1309	bead-like structures	1290:1309	bead-like structures	1290:1309	The spatiotemporal changes observed in the structure of the extracellular material included the formation of a fibrillar network, polymerization of the fibrillar network into a membranous layer, and the presence of bead-like structures on the surface of cells in established graft union.
30999851	2	10	theme	plants	320:325	arg1	differentiation					289:303	cell differentiation	284:303	cell differentiation	284:303	The processes involved in grafting are diverse, and the technique is commonly employed in studies focusing on the mechanisms that regulate cell differentiation or response of plants to abiotic stress.
30999851	2	10	theme	plants	320:325	arg1	response					308:315	response	308:315	response of plants to abiotic stress	308:343	The processes involved in grafting are diverse, and the technique is commonly employed in studies focusing on the mechanisms that regulate cell differentiation or response of plants to abiotic stress.
30999851	10	11	theme	subsequent	1721:1730	arg1	stages					1732:1737	the subsequent stages	1717:1737	the subsequent stages of regeneration	1717:1753	Only three cell wall epitopes, namely: LM19 (un/low-methyl-esterified homogalacturonan), JIM11, and JIM20 (extensins), were detected abundantly on the cut surfaces that made the adhesion plane, as well as in the structure that covered the graft union and in the bead-like structures, during the subsequent stages of regeneration.
30999851	5	12	theme	union	668:672	arg1	surface					647:653	the surface	643:653	the surface of the graft union	643:672	During the study, the formation of a layer that covers the surface of the graft union was observed.
30999851	11	13	theme	Arabidopsis	1943:1953	arg1	grafting					1955:1962	Arabidopsis grafting	1943:1962	Arabidopsis grafting	1943:1962	CONCLUSIONS To the best of our knowledge, this is the first report on the composition and structure of the extracellular material that gets deposited on the surface of graft union during Arabidopsis grafting.
30999851	13	14	theme	potential	2213:2221	arg1	interaction					2239:2249	the potential pectin-extensin interaction	2209:2249	the potential pectin-extensin interaction	2209:2249	The extracellular material is of importance not only due to the potential pectin-extensin interaction but also due to its origin.
30999851	14	15	with	studies	2328:2334	arg1	approach					2353:2360	biochemical approach	2341:2360	biochemical approach for a detailed analysis of the composition and structure of the extracellular material	2341:2447	The findings presented here implicate a need for studies with biochemical approach for a detailed analysis of the composition and structure of the extracellular material.
30999851	7	16	theme	carbohydrate/protein	1044:1063	arg1	material					1065:1072	carbohydrate/protein material	1044:1072	carbohydrate/protein material	1044:1072	RESULTS During grafting, polyphenolic and lipid compounds were detected, along with extracellular deposition of carbohydrate/protein material.
30999851	3	17	from	changes	365:371	arg1	composition					380:390	the composition	376:390	the composition of the cell wall that occur during the grafting process	376:446	Information on the changes in the composition of the cell wall that occur during the grafting process is scarce.
30999851	8	18	theme	network	1237:1243	arg1	formation					1171:1179	the formation	1167:1179	the formation of a fibrillar network	1167:1202	The spatiotemporal changes observed in the structure of the extracellular material included the formation of a fibrillar network, polymerization of the fibrillar network into a membranous layer, and the presence of bead-like structures on the surface of cells in established graft union.
30999851	8	18	theme	network	1237:1243	arg1	polymerization					1205:1218	polymerization	1205:1218	polymerization of the fibrillar network into a membranous layer	1205:1267	The spatiotemporal changes observed in the structure of the extracellular material included the formation of a fibrillar network, polymerization of the fibrillar network into a membranous layer, and the presence of bead-like structures on the surface of cells in established graft union.
30999851	8	18	theme	network	1237:1243	arg1	presence					1278:1285	the presence	1274:1285	the presence of bead-like structures on the surface of cells in established graft union	1274:1360	The spatiotemporal changes observed in the structure of the extracellular material included the formation of a fibrillar network, polymerization of the fibrillar network into a membranous layer, and the presence of bead-like structures on the surface of cells in established graft union.
30999851	6	19	theme	graft	919:923	arg1	union					925:929	the graft union	915:929	the graft union	915:929	So, this study also aimed to describe the histological and cellular changes that accompany autografting of Arabidopsis hypocotyls and to perform preliminary chemical and structural analyses of extracellular material that seals the graft union.
30999851	8	20	theme	bead-like	1290:1298	arg1	structures					1300:1309	bead-like structures	1290:1309	bead-like structures	1290:1309	The spatiotemporal changes observed in the structure of the extracellular material included the formation of a fibrillar network, polymerization of the fibrillar network into a membranous layer, and the presence of bead-like structures on the surface of cells in established graft union.
30999851	11	21	theme	extracellular	1863:1875	arg1	material					1877:1884	the extracellular material	1859:1884	the extracellular material that gets deposited on the surface of graft union during Arabidopsis grafting	1859:1962	CONCLUSIONS To the best of our knowledge, this is the first report on the composition and structure of the extracellular material that gets deposited on the surface of graft union during Arabidopsis grafting.
30999851	11	22	theme	graft	1924:1928	arg1	union					1930:1934	graft union	1924:1934	graft union	1924:1934	CONCLUSIONS To the best of our knowledge, this is the first report on the composition and structure of the extracellular material that gets deposited on the surface of graft union during Arabidopsis grafting.
30999851	8	23	from	presence	1278:1285	arg1	surface					1318:1324	the surface	1314:1324	the surface of cells in established graft union	1314:1360	The spatiotemporal changes observed in the structure of the extracellular material included the formation of a fibrillar network, polymerization of the fibrillar network into a membranous layer, and the presence of bead-like structures on the surface of cells in established graft union.
30999851	11	24	from	report	1816:1821	arg1	structure					1846:1854	structure	1846:1854	structure	1846:1854	CONCLUSIONS To the best of our knowledge, this is the first report on the composition and structure of the extracellular material that gets deposited on the surface of graft union during Arabidopsis grafting.
30999851	11	24	from	report	1816:1821	arg1	composition					1830:1840	composition	1830:1840	composition	1830:1840	CONCLUSIONS To the best of our knowledge, this is the first report on the composition and structure of the extracellular material that gets deposited on the surface of graft union during Arabidopsis grafting.
30999851	8	25	theme	cells	1329:1333	arg1	surface					1318:1324	the surface	1314:1324	the surface of cells in established graft union	1314:1360	The spatiotemporal changes observed in the structure of the extracellular material included the formation of a fibrillar network, polymerization of the fibrillar network into a membranous layer, and the presence of bead-like structures on the surface of cells in established graft union.
30999851	8	26	theme	established	1338:1348	arg1	union					1356:1360	established graft union	1338:1360	established graft union	1338:1360	The spatiotemporal changes observed in the structure of the extracellular material included the formation of a fibrillar network, polymerization of the fibrillar network into a membranous layer, and the presence of bead-like structures on the surface of cells in established graft union.
30999851	1	27	theme	BACKGROUND	81:90	arg1	Grafting					92:99	BACKGROUND Grafting	81:99	BACKGROUND Grafting	81:99	BACKGROUND Grafting is a technique widely used in horticulture.
30999851	1	27	theme	BACKGROUND	81:90	arg1	technique					106:114	a technique	104:114	a technique widely used in horticulture	104:142	BACKGROUND Grafting is a technique widely used in horticulture.
30999851	8	28	theme	structures	1300:1309	arg1	formation					1171:1179	the formation	1167:1179	the formation of a fibrillar network	1167:1202	The spatiotemporal changes observed in the structure of the extracellular material included the formation of a fibrillar network, polymerization of the fibrillar network into a membranous layer, and the presence of bead-like structures on the surface of cells in established graft union.
30999851	8	28	theme	structures	1300:1309	arg1	polymerization					1205:1218	polymerization	1205:1218	polymerization of the fibrillar network into a membranous layer	1205:1267	The spatiotemporal changes observed in the structure of the extracellular material included the formation of a fibrillar network, polymerization of the fibrillar network into a membranous layer, and the presence of bead-like structures on the surface of cells in established graft union.
30999851	8	28	theme	structures	1300:1309	arg1	presence					1278:1285	the presence	1274:1285	the presence of bead-like structures on the surface of cells in established graft union	1274:1360	The spatiotemporal changes observed in the structure of the extracellular material included the formation of a fibrillar network, polymerization of the fibrillar network into a membranous layer, and the presence of bead-like structures on the surface of cells in established graft union.
30999851	3	29	theme	grafting	431:438	arg1	process					440:446	the grafting process	427:446	the grafting process	427:446	Information on the changes in the composition of the cell wall that occur during the grafting process is scarce.
30999851	8	30	from	cells	1329:1333	arg1	union					1356:1360	established graft union	1338:1360	established graft union	1338:1360	The spatiotemporal changes observed in the structure of the extracellular material included the formation of a fibrillar network, polymerization of the fibrillar network into a membranous layer, and the presence of bead-like structures on the surface of cells in established graft union.
30999851	10	31	theme	wall	1442:1445	arg1	epitopes					1447:1454	Only three cell wall epitopes	1426:1454	Only three cell wall epitopes	1426:1454	Only three cell wall epitopes, namely: LM19 (un/low-methyl-esterified homogalacturonan), JIM11, and JIM20 (extensins), were detected abundantly on the cut surfaces that made the adhesion plane, as well as in the structure that covered the graft union and in the bead-like structures, during the subsequent stages of regeneration.
30999851	12	32	theme	sealing	2124:2130	arg1	union					2142:2146	the graft union	2132:2146	sealing the graft union	2124:2146	The results showed that unmethyl-esterified homogalacturonan and extensins are together involved in the adhesion of scion and stock, as well as taking part in sealing the graft union.
30999851	11	33	theme	first	1810:1814	arg1	report					1816:1821	the first report	1806:1821	the first report on the composition and structure of the extracellular material that gets deposited on the surface of graft union during Arabidopsis grafting	1806:1962	CONCLUSIONS To the best of our knowledge, this is the first report on the composition and structure of the extracellular material that gets deposited on the surface of graft union during Arabidopsis grafting.
30999851	0	34	theme	Unmethyl-esterified	0:18	arg1	homogalacturonan					20:35	Unmethyl-esterified homogalacturonan	0:35	Unmethyl-esterified homogalacturonan	0:35	Unmethyl-esterified homogalacturonan and extensins seal Arabidopsis graft union.
30999851	8	35	from	surface	1318:1324	arg1	union					1356:1360	established graft union	1338:1360	established graft union	1338:1360	The spatiotemporal changes observed in the structure of the extracellular material included the formation of a fibrillar network, polymerization of the fibrillar network into a membranous layer, and the presence of bead-like structures on the surface of cells in established graft union.
30999851	11	36	dep	this	1798:1801	arg1	report					1816:1821	the first report	1806:1821	the first report on the composition and structure of the extracellular material that gets deposited on the surface of graft union during Arabidopsis grafting	1806:1962	CONCLUSIONS To the best of our knowledge, this is the first report on the composition and structure of the extracellular material that gets deposited on the surface of graft union during Arabidopsis grafting.
30999851	4	37	theme	cell	534:537	arg1	wall					539:542	the cell wall	530:542	the cell wall using Arabidopsis hypocotyls as an example	530:585	Therefore, this study was carried out for analyzing the composition of the cell wall using Arabidopsis hypocotyls as an example.
30999851	12	38	theme	graft	2136:2140	arg1	union					2142:2146	the graft union	2132:2146	sealing the graft union	2124:2146	The results showed that unmethyl-esterified homogalacturonan and extensins are together involved in the adhesion of scion and stock, as well as taking part in sealing the graft union.
30999851	10	39	dep	epitopes	1447:1454	arg1	detected					1550:1557	detected	1550:1557	were detected abundantly on the cut surfaces that made the adhesion plane, as well as in the structure that covered the graft union and in the bead-like structures, during the subsequent stages of regeneration	1545:1753	Only three cell wall epitopes, namely: LM19 (un/low-methyl-esterified homogalacturonan), JIM11, and JIM20 (extensins), were detected abundantly on the cut surfaces that made the adhesion plane, as well as in the structure that covered the graft union and in the bead-like structures, during the subsequent stages of regeneration.
30999851	6	40	theme	preliminary	833:843	arg1	analyses					869:876	preliminary chemical and structural analyses	833:876	preliminary chemical and structural analyses of extracellular material that seals the graft union	833:929	So, this study also aimed to describe the histological and cellular changes that accompany autografting of Arabidopsis hypocotyls and to perform preliminary chemical and structural analyses of extracellular material that seals the graft union.
30999851	0	41	theme	seal	51:54	arg1	union					74:78	seal Arabidopsis graft union	51:78	seal Arabidopsis graft union	51:78	Unmethyl-esterified homogalacturonan and extensins seal Arabidopsis graft union.
30999851	3	42	theme	cell	399:402	arg1	wall					404:407	the cell wall	395:407	the cell wall that occur during the grafting process	395:446	Information on the changes in the composition of the cell wall that occur during the grafting process is scarce.
30999851	0	43	theme	graft	68:72	arg1	union					74:78	seal Arabidopsis graft union	51:78	seal Arabidopsis graft union	51:78	Unmethyl-esterified homogalacturonan and extensins seal Arabidopsis graft union.
30999851	9	44	theme	bead-like	1369:1377	arg1	structures					1379:1388	These bead-like structures	1363:1388	These bead-like structures	1363:1388	These bead-like structures appeared either "closed" or "open".
30999851	6	45	theme	hypocotyls	807:816	arg1	autografting					779:790	autografting	779:790	autografting of Arabidopsis hypocotyls	779:816	So, this study also aimed to describe the histological and cellular changes that accompany autografting of Arabidopsis hypocotyls and to perform preliminary chemical and structural analyses of extracellular material that seals the graft union.
30999851	12	46	theme	stock	2091:2095	arg1	adhesion					2069:2076	the adhesion	2065:2076	the adhesion of scion and stock, as well as taking part in sealing the graft union	2065:2146	The results showed that unmethyl-esterified homogalacturonan and extensins are together involved in the adhesion of scion and stock, as well as taking part in sealing the graft union.
30999851	8	47	from	formation	1171:1179	arg1	surface					1318:1324	the surface	1314:1324	the surface of cells in established graft union	1314:1360	The spatiotemporal changes observed in the structure of the extracellular material included the formation of a fibrillar network, polymerization of the fibrillar network into a membranous layer, and the presence of bead-like structures on the surface of cells in established graft union.
30999851	8	48	from	polymerization	1205:1218	arg1	surface					1318:1324	the surface	1314:1324	the surface of cells in established graft union	1314:1360	The spatiotemporal changes observed in the structure of the extracellular material included the formation of a fibrillar network, polymerization of the fibrillar network into a membranous layer, and the presence of bead-like structures on the surface of cells in established graft union.
30999851	8	49	theme	material	1149:1156	arg1	structure					1118:1126	the structure	1114:1126	the structure of the extracellular material	1114:1156	The spatiotemporal changes observed in the structure of the extracellular material included the formation of a fibrillar network, polymerization of the fibrillar network into a membranous layer, and the presence of bead-like structures on the surface of cells in established graft union.
30999851	6	50	theme	extracellular	881:893	arg1	material					895:902	extracellular material	881:902	extracellular material	881:902	So, this study also aimed to describe the histological and cellular changes that accompany autografting of Arabidopsis hypocotyls and to perform preliminary chemical and structural analyses of extracellular material that seals the graft union.
30999851	10	51	theme	bead-like	1688:1696	arg1	structures					1698:1707	the bead-like structures	1684:1707	the bead-like structures	1684:1707	Only three cell wall epitopes, namely: LM19 (un/low-methyl-esterified homogalacturonan), JIM11, and JIM20 (extensins), were detected abundantly on the cut surfaces that made the adhesion plane, as well as in the structure that covered the graft union and in the bead-like structures, during the subsequent stages of regeneration.
30999851	6	52	theme	cellular	747:754	arg1	changes					756:762	the histological and cellular changes	726:762	the histological and cellular changes that accompany autografting of Arabidopsis hypocotyls	726:816	So, this study also aimed to describe the histological and cellular changes that accompany autografting of Arabidopsis hypocotyls and to perform preliminary chemical and structural analyses of extracellular material that seals the graft union.
30999851	2	53	theme	cell	284:287	arg1	differentiation					289:303	cell differentiation	284:303	cell differentiation	284:303	The processes involved in grafting are diverse, and the technique is commonly employed in studies focusing on the mechanisms that regulate cell differentiation or response of plants to abiotic stress.
30999851	14	54	theme	detailed	2368:2375	arg1	analysis					2377:2384	a detailed analysis	2366:2384	a detailed analysis of the composition and structure of the extracellular material	2366:2447	The findings presented here implicate a need for studies with biochemical approach for a detailed analysis of the composition and structure of the extracellular material.
30999851	6	55	theme	histological	730:741	arg1	changes					756:762	the histological and cellular changes	726:762	the histological and cellular changes that accompany autografting of Arabidopsis hypocotyls	726:816	So, this study also aimed to describe the histological and cellular changes that accompany autografting of Arabidopsis hypocotyls and to perform preliminary chemical and structural analyses of extracellular material that seals the graft union.
30999851	8	56	theme	fibrillar	1186:1194	arg1	network					1196:1202	a fibrillar network	1184:1202	a fibrillar network	1184:1202	The spatiotemporal changes observed in the structure of the extracellular material included the formation of a fibrillar network, polymerization of the fibrillar network into a membranous layer, and the presence of bead-like structures on the surface of cells in established graft union.
30999851	14	57	theme	material	2440:2447	arg1	composition					2393:2403	composition	2393:2403	composition	2393:2403	The findings presented here implicate a need for studies with biochemical approach for a detailed analysis of the composition and structure of the extracellular material.
30999851	14	57	theme	material	2440:2447	arg1	structure					2409:2417	structure	2409:2417	structure	2409:2417	The findings presented here implicate a need for studies with biochemical approach for a detailed analysis of the composition and structure of the extracellular material.
30999851	8	58	theme	membranous	1252:1261	arg1	layer					1263:1267	a membranous layer	1250:1267	a membranous layer	1250:1267	The spatiotemporal changes observed in the structure of the extracellular material included the formation of a fibrillar network, polymerization of the fibrillar network into a membranous layer, and the presence of bead-like structures on the surface of cells in established graft union.
30999851	5	59	theme	graft	662:666	arg1	union					668:672	the graft union	658:672	the graft union	658:672	During the study, the formation of a layer that covers the surface of the graft union was observed.
30999851	11	60	theme	material	1877:1884	arg1	structure					1846:1854	structure	1846:1854	structure	1846:1854	CONCLUSIONS To the best of our knowledge, this is the first report on the composition and structure of the extracellular material that gets deposited on the surface of graft union during Arabidopsis grafting.
30999851	11	60	theme	material	1877:1884	arg1	composition					1830:1840	composition	1830:1840	composition	1830:1840	CONCLUSIONS To the best of our knowledge, this is the first report on the composition and structure of the extracellular material that gets deposited on the surface of graft union during Arabidopsis grafting.
30999851	13	61	theme	pectin-extensin	2223:2237	arg1	interaction					2239:2249	the potential pectin-extensin interaction	2209:2249	the potential pectin-extensin interaction	2209:2249	The extracellular material is of importance not only due to the potential pectin-extensin interaction but also due to its origin.
30999851	8	62	from	union	1356:1360	arg1	surface					1318:1324	the surface	1314:1324	the surface of cells in established graft union	1314:1360	The spatiotemporal changes observed in the structure of the extracellular material included the formation of a fibrillar network, polymerization of the fibrillar network into a membranous layer, and the presence of bead-like structures on the surface of cells in established graft union.
30999851	10	63	theme	cut	1577:1579	arg1	surfaces					1581:1588	the cut surfaces	1573:1588	the cut surfaces that made the adhesion plane, as well as in the structure that covered the graft union and in the bead-like structures, during the subsequent stages of regeneration	1573:1753	Only three cell wall epitopes, namely: LM19 (un/low-methyl-esterified homogalacturonan), JIM11, and JIM20 (extensins), were detected abundantly on the cut surfaces that made the adhesion plane, as well as in the structure that covered the graft union and in the bead-like structures, during the subsequent stages of regeneration.
30999851	7	64	theme	extracellular	1016:1028	arg1	deposition					1030:1039	extracellular deposition	1016:1039	extracellular deposition of carbohydrate/protein material	1016:1072	RESULTS During grafting, polyphenolic and lipid compounds were detected, along with extracellular deposition of carbohydrate/protein material.
30999851	8	65	theme	fibrillar	1227:1235	arg1	network					1237:1243	the fibrillar network	1223:1243	the fibrillar network	1223:1243	The spatiotemporal changes observed in the structure of the extracellular material included the formation of a fibrillar network, polymerization of the fibrillar network into a membranous layer, and the presence of bead-like structures on the surface of cells in established graft union.
30999851	7	66	theme	material	1065:1072	arg1	deposition					1030:1039	extracellular deposition	1016:1039	extracellular deposition of carbohydrate/protein material	1016:1072	RESULTS During grafting, polyphenolic and lipid compounds were detected, along with extracellular deposition of carbohydrate/protein material.
30999851	14	67	theme	extracellular	2426:2438	arg1	material					2440:2447	the extracellular material	2422:2447	the extracellular material	2422:2447	The findings presented here implicate a need for studies with biochemical approach for a detailed analysis of the composition and structure of the extracellular material.
30999851	12	68	theme	unmethyl-esterified	1989:2007	arg1	homogalacturonan					2009:2024	unmethyl-esterified homogalacturonan	1989:2024	unmethyl-esterified homogalacturonan	1989:2024	The results showed that unmethyl-esterified homogalacturonan and extensins are together involved in the adhesion of scion and stock, as well as taking part in sealing the graft union.
30999851	8	69	located	observed	1102:1109	arg1	structure					1118:1126	the structure	1114:1126	the structure of the extracellular material	1114:1156	The spatiotemporal changes observed in the structure of the extracellular material included the formation of a fibrillar network, polymerization of the fibrillar network into a membranous layer, and the presence of bead-like structures on the surface of cells in established graft union.
30999851	8	69	located	observed	1102:1109	arg2	changes					1094:1100	The spatiotemporal changes	1075:1100	The spatiotemporal changes observed in the structure of the extracellular material	1075:1156	The spatiotemporal changes observed in the structure of the extracellular material included the formation of a fibrillar network, polymerization of the fibrillar network into a membranous layer, and the presence of bead-like structures on the surface of cells in established graft union.
30999851	3	70	from	Information	346:356	arg1	changes					365:371	the changes	361:371	the changes in the composition of the cell wall that occur during the grafting process	361:446	Information on the changes in the composition of the cell wall that occur during the grafting process is scarce.
30999851	7	71	theme	lipid	974:978	arg1	compounds					980:988	polyphenolic and lipid compounds	957:988	compounds	980:988	RESULTS During grafting, polyphenolic and lipid compounds were detected, along with extracellular deposition of carbohydrate/protein material.
30999851	7	71	theme	lipid	974:978	arg1	grafting					947:954	grafting	947:954	grafting	947:954	RESULTS During grafting, polyphenolic and lipid compounds were detected, along with extracellular deposition of carbohydrate/protein material.
30999851	7	72	theme	polyphenolic	957:968	arg1	compounds					980:988	polyphenolic and lipid compounds	957:988	compounds	980:988	RESULTS During grafting, polyphenolic and lipid compounds were detected, along with extracellular deposition of carbohydrate/protein material.
30999851	7	72	theme	polyphenolic	957:968	arg1	grafting					947:954	grafting	947:954	grafting	947:954	RESULTS During grafting, polyphenolic and lipid compounds were detected, along with extracellular deposition of carbohydrate/protein material.
30999851	14	73	dep	composition	2393:2403	arg1	the					2389:2391	the	2389:2391	the	2389:2391	The findings presented here implicate a need for studies with biochemical approach for a detailed analysis of the composition and structure of the extracellular material.
30999851	8	74	theme	graft	1350:1354	arg1	union					1356:1360	established graft union	1338:1360	established graft union	1338:1360	The spatiotemporal changes observed in the structure of the extracellular material included the formation of a fibrillar network, polymerization of the fibrillar network into a membranous layer, and the presence of bead-like structures on the surface of cells in established graft union.
30999851	10	75	theme	adhesion	1604:1611	arg1	plane					1613:1617	the adhesion plane	1600:1617	the adhesion plane	1600:1617	Only three cell wall epitopes, namely: LM19 (un/low-methyl-esterified homogalacturonan), JIM11, and JIM20 (extensins), were detected abundantly on the cut surfaces that made the adhesion plane, as well as in the structure that covered the graft union and in the bead-like structures, during the subsequent stages of regeneration.
30999851	4	76	theme	wall	539:542	arg1	composition					515:525	the composition	511:525	the composition of the cell wall using Arabidopsis hypocotyls as an example	511:585	Therefore, this study was carried out for analyzing the composition of the cell wall using Arabidopsis hypocotyls as an example.
30999851	10	77	theme	cell	1437:1440	arg1	epitopes					1447:1454	Only three cell wall epitopes	1426:1454	Only three cell wall epitopes	1426:1454	Only three cell wall epitopes, namely: LM19 (un/low-methyl-esterified homogalacturonan), JIM11, and JIM20 (extensins), were detected abundantly on the cut surfaces that made the adhesion plane, as well as in the structure that covered the graft union and in the bead-like structures, during the subsequent stages of regeneration.
30999851	8	78	theme	spatiotemporal	1079:1092	arg1	changes					1094:1100	The spatiotemporal changes	1075:1100	The spatiotemporal changes observed in the structure of the extracellular material	1075:1156	The spatiotemporal changes observed in the structure of the extracellular material included the formation of a fibrillar network, polymerization of the fibrillar network into a membranous layer, and the presence of bead-like structures on the surface of cells in established graft union.
30999851	10	79	theme	regeneration	1742:1753	arg1	stages					1732:1737	the subsequent stages	1717:1737	the subsequent stages of regeneration	1717:1753	Only three cell wall epitopes, namely: LM19 (un/low-methyl-esterified homogalacturonan), JIM11, and JIM20 (extensins), were detected abundantly on the cut surfaces that made the adhesion plane, as well as in the structure that covered the graft union and in the bead-like structures, during the subsequent stages of regeneration.
30999851	4	80	theme	Arabidopsis	550:560	arg1	hypocotyls					562:571	Arabidopsis hypocotyls	550:571	Arabidopsis hypocotyls	550:571	Therefore, this study was carried out for analyzing the composition of the cell wall using Arabidopsis hypocotyls as an example.
30999851	4	80	theme	Arabidopsis	550:560	arg1	example					579:585	an example	576:585	an example	576:585	Therefore, this study was carried out for analyzing the composition of the cell wall using Arabidopsis hypocotyls as an example.
30999851	0	81	theme	Arabidopsis	56:66	arg1	union					74:78	seal Arabidopsis graft union	51:78	seal Arabidopsis graft union	51:78	Unmethyl-esterified homogalacturonan and extensins seal Arabidopsis graft union.
30999851	13	82	theme	extracellular	2153:2165	arg1	material					2167:2174	The extracellular material	2149:2174	The extracellular material	2149:2174	The extracellular material is of importance not only due to the potential pectin-extensin interaction but also due to its origin.
30999851	0	83	dep	union	74:78	arg1	extensins					41:49	extensins	41:49	extensins	41:49	Unmethyl-esterified homogalacturonan and extensins seal Arabidopsis graft union.
30999851	0	83	dep	union	74:78	arg1	homogalacturonan					20:35	Unmethyl-esterified homogalacturonan	0:35	Unmethyl-esterified homogalacturonan	0:35	Unmethyl-esterified homogalacturonan and extensins seal Arabidopsis graft union.
30999851	3	84	theme	wall	404:407	arg1	composition					380:390	the composition	376:390	the composition of the cell wall that occur during the grafting process	376:446	Information on the changes in the composition of the cell wall that occur during the grafting process is scarce.
30999851	14	85	theme	structure	2409:2417	arg1	analysis					2377:2384	a detailed analysis	2366:2384	a detailed analysis of the composition and structure of the extracellular material	2366:2447	The findings presented here implicate a need for studies with biochemical approach for a detailed analysis of the composition and structure of the extracellular material.
30999851	14	86	theme	biochemical	2341:2351	arg1	approach					2353:2360	biochemical approach	2341:2360	biochemical approach for a detailed analysis of the composition and structure of the extracellular material	2341:2447	The findings presented here implicate a need for studies with biochemical approach for a detailed analysis of the composition and structure of the extracellular material.
30999851	12	87	from	part	2116:2119	arg1	union					2142:2146	the graft union	2132:2146	sealing the graft union	2124:2146	The results showed that unmethyl-esterified homogalacturonan and extensins are together involved in the adhesion of scion and stock, as well as taking part in sealing the graft union.
30999851	6	88	theme	Arabidopsis	795:805	arg1	hypocotyls					807:816	Arabidopsis hypocotyls	795:816	Arabidopsis hypocotyls	795:816	So, this study also aimed to describe the histological and cellular changes that accompany autografting of Arabidopsis hypocotyls and to perform preliminary chemical and structural analyses of extracellular material that seals the graft union.
30999851	11	89	dep	composition	1830:1840	arg1	the					1826:1828	the	1826:1828	the	1826:1828	CONCLUSIONS To the best of our knowledge, this is the first report on the composition and structure of the extracellular material that gets deposited on the surface of graft union during Arabidopsis grafting.
30999851	10	90	theme	un/low-methyl-esterified	1471:1494	arg1	LM19					1465:1468	LM19	1465:1468	LM19	1465:1468	Only three cell wall epitopes, namely: LM19 (un/low-methyl-esterified homogalacturonan), JIM11, and JIM20 (extensins), were detected abundantly on the cut surfaces that made the adhesion plane, as well as in the structure that covered the graft union and in the bead-like structures, during the subsequent stages of regeneration.
30999851	10	90	theme	un/low-methyl-esterified	1471:1494	arg1	homogalacturonan					1496:1511	un/low-methyl-esterified homogalacturonan	1471:1511	un/low-methyl-esterified homogalacturonan	1471:1511	Only three cell wall epitopes, namely: LM19 (un/low-methyl-esterified homogalacturonan), JIM11, and JIM20 (extensins), were detected abundantly on the cut surfaces that made the adhesion plane, as well as in the structure that covered the graft union and in the bead-like structures, during the subsequent stages of regeneration.
30999851	2	91	theme	abiotic	330:336	arg1	stress					338:343	abiotic stress	330:343	abiotic stress	330:343	The processes involved in grafting are diverse, and the technique is commonly employed in studies focusing on the mechanisms that regulate cell differentiation or response of plants to abiotic stress.
30999851	8	92	theme	extracellular	1135:1147	arg1	material					1149:1156	the extracellular material	1131:1156	the extracellular material	1131:1156	The spatiotemporal changes observed in the structure of the extracellular material included the formation of a fibrillar network, polymerization of the fibrillar network into a membranous layer, and the presence of bead-like structures on the surface of cells in established graft union.
30999851	10	93	located	detected	1550:1557	arg2	LM19					1465:1468	LM19	1465:1468	LM19	1465:1468	Only three cell wall epitopes, namely: LM19 (un/low-methyl-esterified homogalacturonan), JIM11, and JIM20 (extensins), were detected abundantly on the cut surfaces that made the adhesion plane, as well as in the structure that covered the graft union and in the bead-like structures, during the subsequent stages of regeneration.
30999851	10	93	located	detected	1550:1557	arg2	JIM11					1515:1519	JIM11	1515:1519	JIM11	1515:1519	Only three cell wall epitopes, namely: LM19 (un/low-methyl-esterified homogalacturonan), JIM11, and JIM20 (extensins), were detected abundantly on the cut surfaces that made the adhesion plane, as well as in the structure that covered the graft union and in the bead-like structures, during the subsequent stages of regeneration.
30999851	10	93	located	detected	1550:1557	arg2	homogalacturonan					1496:1511	un/low-methyl-esterified homogalacturonan	1471:1511	un/low-methyl-esterified homogalacturonan	1471:1511	Only three cell wall epitopes, namely: LM19 (un/low-methyl-esterified homogalacturonan), JIM11, and JIM20 (extensins), were detected abundantly on the cut surfaces that made the adhesion plane, as well as in the structure that covered the graft union and in the bead-like structures, during the subsequent stages of regeneration.
30999851	10	93	located	detected	1550:1557	arg2	JIM20					1526:1530	JIM20	1526:1530	JIM20	1526:1530	Only three cell wall epitopes, namely: LM19 (un/low-methyl-esterified homogalacturonan), JIM11, and JIM20 (extensins), were detected abundantly on the cut surfaces that made the adhesion plane, as well as in the structure that covered the graft union and in the bead-like structures, during the subsequent stages of regeneration.
30999851	10	93	located	detected	1550:1557	arg2	extensins					1533:1541	extensins	1533:1541	extensins	1533:1541	Only three cell wall epitopes, namely: LM19 (un/low-methyl-esterified homogalacturonan), JIM11, and JIM20 (extensins), were detected abundantly on the cut surfaces that made the adhesion plane, as well as in the structure that covered the graft union and in the bead-like structures, during the subsequent stages of regeneration.
30999851	10	93	located	detected	1550:1557	arg1	surfaces					1581:1588	the cut surfaces	1573:1588	the cut surfaces that made the adhesion plane, as well as in the structure that covered the graft union and in the bead-like structures, during the subsequent stages of regeneration	1573:1753	Only three cell wall epitopes, namely: LM19 (un/low-methyl-esterified homogalacturonan), JIM11, and JIM20 (extensins), were detected abundantly on the cut surfaces that made the adhesion plane, as well as in the structure that covered the graft union and in the bead-like structures, during the subsequent stages of regeneration.
30999851	10	94	theme	graft	1665:1669	arg1	union					1671:1675	the graft union	1661:1675	the graft union	1661:1675	Only three cell wall epitopes, namely: LM19 (un/low-methyl-esterified homogalacturonan), JIM11, and JIM20 (extensins), were detected abundantly on the cut surfaces that made the adhesion plane, as well as in the structure that covered the graft union and in the bead-like structures, during the subsequent stages of regeneration.
30999851	9	95	theme	closed	1407:1412	arg1	"					1413:1413	"closed"	1406:1413	"closed"	1406:1413	These bead-like structures appeared either "closed" or "open".
31721238	9	0	theme	Dietary	1561:1567	arg1	CS					1569:1570	Dietary CS	1561:1570	Dietary CS	1561:1570	CONCLUSION Dietary CS and RS alter the microbial composition and diversity, and modulate the metabolic pathways of microbial metabolism in caecum of broilers, which may further affect nutrient utilization and hindgut health of the host.
31721238	6	1	theme	NC	986:987	arg1	group					989:993	the NC group	982:993	the NC group	982:993	RESULTS The CS group increased the abundances of Bilophila, Eggerthella, Olsenella and Sellimonas and decreased proportion of Akkermansia, Eisenbergiella, Oscillospira, Ruminococcaceae NK4A214 group and Synergistes in the caecum of birds compared to the NC group.
31721238	10	2	theme	Chemical	1805:1812	arg1	Industry					1814:1821	Chemical Industry	1805:1821	Chemical Industry	1805:1821	© 2019 Society of Chemical Industry.
31721238	6	3	dep	Olsenella	805:813	arg1	Eggerthella					792:802	Eggerthella	792:802	Eggerthella	792:802	RESULTS The CS group increased the abundances of Bilophila, Eggerthella, Olsenella and Sellimonas and decreased proportion of Akkermansia, Eisenbergiella, Oscillospira, Ruminococcaceae NK4A214 group and Synergistes in the caecum of birds compared to the NC group.
31721238	7	4	contain	had	1030:1032	arg2	abundances					1124:1133	lower abundances	1118:1133	lower abundances of Alistipes, Bilophila, Christensenellaceae R-7 group, Eggerthella and Ruminiclostridium 1	1118:1225	However, the birds from RS groups had higher abundances of Anaerofilum, Bacteroides, Desulfovibrio and Parasutterella and lower abundances of Alistipes, Bilophila, Christensenellaceae R-7 group, Eggerthella and Ruminiclostridium 1 than the CS group.
31721238	7	4	contain	had	1030:1032	arg2	abundances					1041:1050	higher abundances	1034:1050	higher abundances of Anaerofilum, Bacteroides, Desulfovibrio and Parasutterella	1034:1112	However, the birds from RS groups had higher abundances of Anaerofilum, Bacteroides, Desulfovibrio and Parasutterella and lower abundances of Alistipes, Bilophila, Christensenellaceae R-7 group, Eggerthella and Ruminiclostridium 1 than the CS group.
31721238	7	4	contain	had	1030:1032	arg1	birds					1009:1013	the birds	1005:1013	the birds from RS groups	1005:1028	However, the birds from RS groups had higher abundances of Anaerofilum, Bacteroides, Desulfovibrio and Parasutterella and lower abundances of Alistipes, Bilophila, Christensenellaceae R-7 group, Eggerthella and Ruminiclostridium 1 than the CS group.
31721238	6	5	theme	birds	964:968	arg1	caecum					954:959	the caecum	950:959	the caecum of birds	950:968	RESULTS The CS group increased the abundances of Bilophila, Eggerthella, Olsenella and Sellimonas and decreased proportion of Akkermansia, Eisenbergiella, Oscillospira, Ruminococcaceae NK4A214 group and Synergistes in the caecum of birds compared to the NC group.
31721238	4	6	theme	12	569:570	arg1	%					571:571	%	571:571	%	571:571	A total of 320, 1-day-old male Arbor Acres broiler chicks were randomly assigned into five groups including normal corn-soybean (NC) diet, corn starch (CS) diet group, 4%, 8% and 12% resistant starch (RS) diet groups.
31721238	8	7	from	microbiota	1288:1297	arg1	prediction					1257:1266	Functional prediction	1246:1266	Functional prediction of these changes in microbiota	1246:1297	Functional prediction of these changes in microbiota revealed that the CS diet drove caecal microbiota that were more inclined to utilize carbohydrates through glycolysis/gluconeogenesis metabolism, while the 8%RS and 12%RS diets depleted microbial glycolysis/gluconeogenesis and amino acids metabolism.
31721238	1	8	theme	gut	167:169	arg1	microbiota					171:180	gut microbiota	167:180	gut microbiota	167:180	BACKGROUND Diet plays an important role in shaping the composition of gut microbiota.
31721238	9	9	theme	microbial	1589:1597	arg1	composition					1599:1609	the microbial composition	1585:1609	the microbial composition	1585:1609	CONCLUSION Dietary CS and RS alter the microbial composition and diversity, and modulate the metabolic pathways of microbial metabolism in caecum of broilers, which may further affect nutrient utilization and hindgut health of the host.
31721238	4	10	theme	resistant	573:581	arg1	RS					591:592	RS	591:592	RS	591:592	A total of 320, 1-day-old male Arbor Acres broiler chicks were randomly assigned into five groups including normal corn-soybean (NC) diet, corn starch (CS) diet group, 4%, 8% and 12% resistant starch (RS) diet groups.
31721238	4	10	theme	resistant	573:581	arg1	starch					583:588	12% resistant starch	569:588	12% resistant starch (RS) diet groups	569:605	A total of 320, 1-day-old male Arbor Acres broiler chicks were randomly assigned into five groups including normal corn-soybean (NC) diet, corn starch (CS) diet group, 4%, 8% and 12% resistant starch (RS) diet groups.
31721238	8	11	theme	caecal	1331:1336	arg1	microbiota					1338:1347	caecal microbiota	1331:1347	caecal microbiota that were more inclined to utilize carbohydrates through glycolysis/gluconeogenesis metabolism, while the 8%RS and 12%RS diets depleted microbial glycolysis/gluconeogenesis and amino acids metabolism	1331:1547	Functional prediction of these changes in microbiota revealed that the CS diet drove caecal microbiota that were more inclined to utilize carbohydrates through glycolysis/gluconeogenesis metabolism, while the 8%RS and 12%RS diets depleted microbial glycolysis/gluconeogenesis and amino acids metabolism.
31721238	2	12	theme	carbohydrates	212:224	arg1	source					202:207	the main source	193:207	the main source of carbohydrates in diets of broilers	193:245	Starch is the main source of carbohydrates in diets of broilers.
31721238	2	12	theme	carbohydrates	212:224	arg1	Starch					183:188	Starch	183:188	Starch	183:188	Starch is the main source of carbohydrates in diets of broilers.
31721238	3	13	theme	dietary	287:293	arg1	starch					300:305	dietary corn starch	287:305	dietary corn starch	287:305	This study investigated the effects of dietary corn starch and resistant starch on composition and function of caecal microbiota of broilers.
31721238	4	14	theme	Acres	427:431	arg1	chicks					441:446	320, 1-day-old male Arbor Acres broiler chicks	401:446	chicks	441:446	A total of 320, 1-day-old male Arbor Acres broiler chicks were randomly assigned into five groups including normal corn-soybean (NC) diet, corn starch (CS) diet group, 4%, 8% and 12% resistant starch (RS) diet groups.
31721238	9	15	theme	metabolic	1643:1651	arg1	pathways					1653:1660	the metabolic pathways	1639:1660	the metabolic pathways of microbial metabolism	1639:1684	CONCLUSION Dietary CS and RS alter the microbial composition and diversity, and modulate the metabolic pathways of microbial metabolism in caecum of broilers, which may further affect nutrient utilization and hindgut health of the host.
31721238	2	16	theme	broilers	238:245	arg1	diets					229:233	diets	229:233	diets of broilers	229:245	Starch is the main source of carbohydrates in diets of broilers.
31721238	6	17	theme	group	925:929	arg1	Synergistes					935:945	Synergistes	935:945	Synergistes	935:945	RESULTS The CS group increased the abundances of Bilophila, Eggerthella, Olsenella and Sellimonas and decreased proportion of Akkermansia, Eisenbergiella, Oscillospira, Ruminococcaceae NK4A214 group and Synergistes in the caecum of birds compared to the NC group.
31721238	6	17	theme	group	925:929	arg1	proportion					844:853	proportion	844:853	proportion of Akkermansia, Eisenbergiella, Oscillospira, Ruminococcaceae NK4A214 group	844:929	RESULTS The CS group increased the abundances of Bilophila, Eggerthella, Olsenella and Sellimonas and decreased proportion of Akkermansia, Eisenbergiella, Oscillospira, Ruminococcaceae NK4A214 group and Synergistes in the caecum of birds compared to the NC group.
31721238	3	18	theme	resistant	311:319	arg1	starch					321:326	resistant starch	311:326	resistant starch	311:326	This study investigated the effects of dietary corn starch and resistant starch on composition and function of caecal microbiota of broilers.
31721238	7	19	theme	CS	1236:1237	arg1	group					1239:1243	the CS group	1232:1243	the CS group	1232:1243	However, the birds from RS groups had higher abundances of Anaerofilum, Bacteroides, Desulfovibrio and Parasutterella and lower abundances of Alistipes, Bilophila, Christensenellaceae R-7 group, Eggerthella and Ruminiclostridium 1 than the CS group.
31721238	5	20	theme	broilers	642:649	arg1	contents					619:626	The caecal contents	608:626	The caecal contents of 42-day old broilers	608:649	The caecal contents of 42-day old broilers were sampled and microbiota community was analysed with 16S rRNA gene sequences.
31721238	6	21	theme	Ruminococcaceae	901:915	arg1	group					925:929	Akkermansia, Eisenbergiella, Oscillospira, Ruminococcaceae NK4A214 group	858:929	Akkermansia, Eisenbergiella, Oscillospira, Ruminococcaceae NK4A214 group	858:929	RESULTS The CS group increased the abundances of Bilophila, Eggerthella, Olsenella and Sellimonas and decreased proportion of Akkermansia, Eisenbergiella, Oscillospira, Ruminococcaceae NK4A214 group and Synergistes in the caecum of birds compared to the NC group.
31721238	3	22	theme	starch	300:305	arg1	effects					276:282	the effects	272:282	the effects of dietary corn starch and resistant starch on composition and function of caecal microbiota of broilers	272:387	This study investigated the effects of dietary corn starch and resistant starch on composition and function of caecal microbiota of broilers.
31721238	4	23	theme	corn	529:532	arg1	CS					542:543	CS	542:543	CS	542:543	A total of 320, 1-day-old male Arbor Acres broiler chicks were randomly assigned into five groups including normal corn-soybean (NC) diet, corn starch (CS) diet group, 4%, 8% and 12% resistant starch (RS) diet groups.
31721238	4	23	theme	corn	529:532	arg1	starch					534:539	corn starch	529:539	corn starch (CS) diet group	529:555	A total of 320, 1-day-old male Arbor Acres broiler chicks were randomly assigned into five groups including normal corn-soybean (NC) diet, corn starch (CS) diet group, 4%, 8% and 12% resistant starch (RS) diet groups.
31721238	0	24	theme	microbiota	73:82	arg1	composition					38:48	composition	38:48	composition	38:48	Dietary resistant starch modifies the composition and function of caecal microbiota of broilers.
31721238	0	24	theme	microbiota	73:82	arg1	function					54:61	function	54:61	function	54:61	Dietary resistant starch modifies the composition and function of caecal microbiota of broilers.
31721238	8	25	theme	glycolysis/gluconeogenesis	1406:1431	arg1	metabolism					1433:1442	glycolysis/gluconeogenesis metabolism	1406:1442	glycolysis/gluconeogenesis metabolism	1406:1442	Functional prediction of these changes in microbiota revealed that the CS diet drove caecal microbiota that were more inclined to utilize carbohydrates through glycolysis/gluconeogenesis metabolism, while the 8%RS and 12%RS diets depleted microbial glycolysis/gluconeogenesis and amino acids metabolism.
31721238	0	26	theme	broilers	87:94	arg1	microbiota					73:82	caecal microbiota	66:82	caecal microbiota of broilers	66:94	Dietary resistant starch modifies the composition and function of caecal microbiota of broilers.
31721238	9	27	theme	broilers	1699:1706	arg1	caecum					1689:1694	caecum	1689:1694	caecum	1689:1694	CONCLUSION Dietary CS and RS alter the microbial composition and diversity, and modulate the metabolic pathways of microbial metabolism in caecum of broilers, which may further affect nutrient utilization and hindgut health of the host.
31721238	4	28	theme	chicks	441:446	arg1	total					392:396	A total	390:396	A total of 320, 1-day-old male Arbor Acres broiler chicks	390:446	A total of 320, 1-day-old male Arbor Acres broiler chicks were randomly assigned into five groups including normal corn-soybean (NC) diet, corn starch (CS) diet group, 4%, 8% and 12% resistant starch (RS) diet groups.
31721238	8	29	theme	Functional	1246:1255	arg1	prediction					1257:1266	Functional prediction	1246:1266	Functional prediction of these changes in microbiota	1246:1297	Functional prediction of these changes in microbiota revealed that the CS diet drove caecal microbiota that were more inclined to utilize carbohydrates through glycolysis/gluconeogenesis metabolism, while the 8%RS and 12%RS diets depleted microbial glycolysis/gluconeogenesis and amino acids metabolism.
31721238	7	30	theme	Bacteroides	1068:1078	arg1	abundances					1041:1050	higher abundances	1034:1050	higher abundances of Anaerofilum, Bacteroides, Desulfovibrio and Parasutterella	1034:1112	However, the birds from RS groups had higher abundances of Anaerofilum, Bacteroides, Desulfovibrio and Parasutterella and lower abundances of Alistipes, Bilophila, Christensenellaceae R-7 group, Eggerthella and Ruminiclostridium 1 than the CS group.
31721238	7	30	theme	Bacteroides	1068:1078	arg1	abundances					1124:1133	lower abundances	1118:1133	lower abundances of Alistipes, Bilophila, Christensenellaceae R-7 group, Eggerthella and Ruminiclostridium 1	1118:1225	However, the birds from RS groups had higher abundances of Anaerofilum, Bacteroides, Desulfovibrio and Parasutterella and lower abundances of Alistipes, Bilophila, Christensenellaceae R-7 group, Eggerthella and Ruminiclostridium 1 than the CS group.
31721238	8	31	dep	glycolysis/gluconeogenesis	1495:1520	arg1	metabolism					1538:1547	metabolism	1538:1547	metabolism	1538:1547	Functional prediction of these changes in microbiota revealed that the CS diet drove caecal microbiota that were more inclined to utilize carbohydrates through glycolysis/gluconeogenesis metabolism, while the 8%RS and 12%RS diets depleted microbial glycolysis/gluconeogenesis and amino acids metabolism.
31721238	3	32	theme	microbiota	366:375	arg1	composition					331:341	composition	331:341	composition	331:341	This study investigated the effects of dietary corn starch and resistant starch on composition and function of caecal microbiota of broilers.
31721238	3	32	theme	microbiota	366:375	arg1	function					347:354	function	347:354	function	347:354	This study investigated the effects of dietary corn starch and resistant starch on composition and function of caecal microbiota of broilers.
31721238	0	33	theme	caecal	66:71	arg1	microbiota					73:82	caecal microbiota	66:82	caecal microbiota of broilers	66:94	Dietary resistant starch modifies the composition and function of caecal microbiota of broilers.
31721238	0	34	theme	Dietary	0:6	arg1	starch					18:23	Dietary resistant starch	0:23	Dietary resistant starch	0:23	Dietary resistant starch modifies the composition and function of caecal microbiota of broilers.
31721238	8	35	theme	%	1456:1456	arg1	RS					1457:1458	8%RS	1455:1458	8%RS	1455:1458	Functional prediction of these changes in microbiota revealed that the CS diet drove caecal microbiota that were more inclined to utilize carbohydrates through glycolysis/gluconeogenesis metabolism, while the 8%RS and 12%RS diets depleted microbial glycolysis/gluconeogenesis and amino acids metabolism.
31721238	8	36	theme	changes	1277:1283	arg1	prediction					1257:1266	Functional prediction	1246:1266	Functional prediction of these changes in microbiota	1246:1297	Functional prediction of these changes in microbiota revealed that the CS diet drove caecal microbiota that were more inclined to utilize carbohydrates through glycolysis/gluconeogenesis metabolism, while the 8%RS and 12%RS diets depleted microbial glycolysis/gluconeogenesis and amino acids metabolism.
31721238	5	37	theme	rRNA	711:714	arg1	sequences					721:729	16S rRNA gene sequences	707:729	16S rRNA gene sequences	707:729	The caecal contents of 42-day old broilers were sampled and microbiota community was analysed with 16S rRNA gene sequences.
31721238	9	38	theme	nutrient	1734:1741	arg1	utilization					1743:1753	nutrient utilization	1734:1753	nutrient utilization	1734:1753	CONCLUSION Dietary CS and RS alter the microbial composition and diversity, and modulate the metabolic pathways of microbial metabolism in caecum of broilers, which may further affect nutrient utilization and hindgut health of the host.
31721238	5	39	theme	microbiota	668:677	arg1	community					679:687	microbiota community	668:687	microbiota community	668:687	The caecal contents of 42-day old broilers were sampled and microbiota community was analysed with 16S rRNA gene sequences.
31721238	6	40	theme	CS	744:745	arg1	group					747:751	The CS group	740:751	The CS group	740:751	RESULTS The CS group increased the abundances of Bilophila, Eggerthella, Olsenella and Sellimonas and decreased proportion of Akkermansia, Eisenbergiella, Oscillospira, Ruminococcaceae NK4A214 group and Synergistes in the caecum of birds compared to the NC group.
31721238	3	41	theme	broilers	380:387	arg1	microbiota					366:375	caecal microbiota	359:375	caecal microbiota of broilers	359:387	This study investigated the effects of dietary corn starch and resistant starch on composition and function of caecal microbiota of broilers.
31721238	8	42	theme	%	1466:1466	arg1	RS					1467:1468	12%RS	1464:1468	12%RS	1464:1468	Functional prediction of these changes in microbiota revealed that the CS diet drove caecal microbiota that were more inclined to utilize carbohydrates through glycolysis/gluconeogenesis metabolism, while the 8%RS and 12%RS diets depleted microbial glycolysis/gluconeogenesis and amino acids metabolism.
31721238	7	43	theme	lower	1118:1122	arg1	abundances					1124:1133	lower abundances	1118:1133	lower abundances of Alistipes, Bilophila, Christensenellaceae R-7 group, Eggerthella and Ruminiclostridium 1	1118:1225	However, the birds from RS groups had higher abundances of Anaerofilum, Bacteroides, Desulfovibrio and Parasutterella and lower abundances of Alistipes, Bilophila, Christensenellaceae R-7 group, Eggerthella and Ruminiclostridium 1 than the CS group.
31721238	7	44	theme	RS	1020:1021	arg1	groups					1023:1028	RS groups	1020:1028	RS groups	1020:1028	However, the birds from RS groups had higher abundances of Anaerofilum, Bacteroides, Desulfovibrio and Parasutterella and lower abundances of Alistipes, Bilophila, Christensenellaceae R-7 group, Eggerthella and Ruminiclostridium 1 than the CS group.
31721238	8	45	theme	CS	1317:1318	arg1	diet					1320:1323	the CS diet	1313:1323	the CS diet	1313:1323	Functional prediction of these changes in microbiota revealed that the CS diet drove caecal microbiota that were more inclined to utilize carbohydrates through glycolysis/gluconeogenesis metabolism, while the 8%RS and 12%RS diets depleted microbial glycolysis/gluconeogenesis and amino acids metabolism.
31721238	3	46	from	effects	276:282	arg1	composition					331:341	composition	331:341	composition	331:341	This study investigated the effects of dietary corn starch and resistant starch on composition and function of caecal microbiota of broilers.
31721238	3	46	from	effects	276:282	arg1	function					347:354	function	347:354	function	347:354	This study investigated the effects of dietary corn starch and resistant starch on composition and function of caecal microbiota of broilers.
31721238	8	47	theme	microbial	1485:1493	arg1	glycolysis/gluconeogenesis					1495:1520	microbial glycolysis/gluconeogenesis	1485:1520	microbial glycolysis/gluconeogenesis	1485:1520	Functional prediction of these changes in microbiota revealed that the CS diet drove caecal microbiota that were more inclined to utilize carbohydrates through glycolysis/gluconeogenesis metabolism, while the 8%RS and 12%RS diets depleted microbial glycolysis/gluconeogenesis and amino acids metabolism.
31721238	6	48	theme	Olsenella	805:813	arg1	abundances					767:776	the abundances	763:776	the abundances of Bilophila, Eggerthella, Olsenella and Sellimonas	763:828	RESULTS The CS group increased the abundances of Bilophila, Eggerthella, Olsenella and Sellimonas and decreased proportion of Akkermansia, Eisenbergiella, Oscillospira, Ruminococcaceae NK4A214 group and Synergistes in the caecum of birds compared to the NC group.
31721238	4	49	theme	normal	498:503	arg1	diet					523:526	normal corn-soybean (NC) diet	498:526	normal corn-soybean (NC) diet	498:526	A total of 320, 1-day-old male Arbor Acres broiler chicks were randomly assigned into five groups including normal corn-soybean (NC) diet, corn starch (CS) diet group, 4%, 8% and 12% resistant starch (RS) diet groups.
31721238	9	50	theme	metabolism	1675:1684	arg1	pathways					1653:1660	the metabolic pathways	1639:1660	the metabolic pathways of microbial metabolism	1639:1684	CONCLUSION Dietary CS and RS alter the microbial composition and diversity, and modulate the metabolic pathways of microbial metabolism in caecum of broilers, which may further affect nutrient utilization and hindgut health of the host.
31721238	4	51	theme	starch	534:539	arg1	group					551:555	corn starch (CS) diet group	529:555	corn starch (CS) diet group	529:555	A total of 320, 1-day-old male Arbor Acres broiler chicks were randomly assigned into five groups including normal corn-soybean (NC) diet, corn starch (CS) diet group, 4%, 8% and 12% resistant starch (RS) diet groups.
31721238	4	51	theme	starch	534:539	arg1	groups					481:486	five groups	476:486	five groups including normal corn-soybean (NC) diet	476:526	A total of 320, 1-day-old male Arbor Acres broiler chicks were randomly assigned into five groups including normal corn-soybean (NC) diet, corn starch (CS) diet group, 4%, 8% and 12% resistant starch (RS) diet groups.
31721238	8	52	from	prediction	1257:1266	arg1	microbiota					1288:1297	microbiota	1288:1297	microbiota	1288:1297	Functional prediction of these changes in microbiota revealed that the CS diet drove caecal microbiota that were more inclined to utilize carbohydrates through glycolysis/gluconeogenesis metabolism, while the 8%RS and 12%RS diets depleted microbial glycolysis/gluconeogenesis and amino acids metabolism.
31721238	7	53	theme	Parasutterella	1099:1112	arg1	abundances					1041:1050	higher abundances	1034:1050	higher abundances of Anaerofilum, Bacteroides, Desulfovibrio and Parasutterella	1034:1112	However, the birds from RS groups had higher abundances of Anaerofilum, Bacteroides, Desulfovibrio and Parasutterella and lower abundances of Alistipes, Bilophila, Christensenellaceae R-7 group, Eggerthella and Ruminiclostridium 1 than the CS group.
31721238	7	53	theme	Parasutterella	1099:1112	arg1	abundances					1124:1133	lower abundances	1118:1133	lower abundances of Alistipes, Bilophila, Christensenellaceae R-7 group, Eggerthella and Ruminiclostridium 1	1118:1225	However, the birds from RS groups had higher abundances of Anaerofilum, Bacteroides, Desulfovibrio and Parasutterella and lower abundances of Alistipes, Bilophila, Christensenellaceae R-7 group, Eggerthella and Ruminiclostridium 1 than the CS group.
31721238	1	54	theme	microbiota	171:180	arg1	composition					152:162	the composition	148:162	the composition of gut microbiota	148:180	BACKGROUND Diet plays an important role in shaping the composition of gut microbiota.
31721238	4	55	theme	%	571:571	arg1	RS					591:592	RS	591:592	RS	591:592	A total of 320, 1-day-old male Arbor Acres broiler chicks were randomly assigned into five groups including normal corn-soybean (NC) diet, corn starch (CS) diet group, 4%, 8% and 12% resistant starch (RS) diet groups.
31721238	4	55	theme	%	571:571	arg1	starch					583:588	12% resistant starch	569:588	12% resistant starch (RS) diet groups	569:605	A total of 320, 1-day-old male Arbor Acres broiler chicks were randomly assigned into five groups including normal corn-soybean (NC) diet, corn starch (CS) diet group, 4%, 8% and 12% resistant starch (RS) diet groups.
31721238	5	56	theme	gene	716:719	arg1	sequences					721:729	16S rRNA gene sequences	707:729	16S rRNA gene sequences	707:729	The caecal contents of 42-day old broilers were sampled and microbiota community was analysed with 16S rRNA gene sequences.
31721238	4	57	theme	Arbor	421:425	arg1	chicks					441:446	320, 1-day-old male Arbor Acres broiler chicks	401:446	chicks	441:446	A total of 320, 1-day-old male Arbor Acres broiler chicks were randomly assigned into five groups including normal corn-soybean (NC) diet, corn starch (CS) diet group, 4%, 8% and 12% resistant starch (RS) diet groups.
31721238	8	58	theme	12	1464:1465	arg1	%					1466:1466	%	1466:1466	%	1466:1466	Functional prediction of these changes in microbiota revealed that the CS diet drove caecal microbiota that were more inclined to utilize carbohydrates through glycolysis/gluconeogenesis metabolism, while the 8%RS and 12%RS diets depleted microbial glycolysis/gluconeogenesis and amino acids metabolism.
31721238	9	59	dep	CONCLUSION	1550:1559	arg1	RS					1576:1577	RS	1576:1577	RS	1576:1577	CONCLUSION Dietary CS and RS alter the microbial composition and diversity, and modulate the metabolic pathways of microbial metabolism in caecum of broilers, which may further affect nutrient utilization and hindgut health of the host.
31721238	9	59	dep	CONCLUSION	1550:1559	arg1	CS					1569:1570	Dietary CS	1561:1570	Dietary CS	1561:1570	CONCLUSION Dietary CS and RS alter the microbial composition and diversity, and modulate the metabolic pathways of microbial metabolism in caecum of broilers, which may further affect nutrient utilization and hindgut health of the host.
31721238	9	60	theme	microbial	1665:1673	arg1	metabolism					1675:1684	microbial metabolism	1665:1684	microbial metabolism	1665:1684	CONCLUSION Dietary CS and RS alter the microbial composition and diversity, and modulate the metabolic pathways of microbial metabolism in caecum of broilers, which may further affect nutrient utilization and hindgut health of the host.
31721238	4	61	theme	broiler	433:439	arg1	chicks					441:446	320, 1-day-old male Arbor Acres broiler chicks	401:446	chicks	441:446	A total of 320, 1-day-old male Arbor Acres broiler chicks were randomly assigned into five groups including normal corn-soybean (NC) diet, corn starch (CS) diet group, 4%, 8% and 12% resistant starch (RS) diet groups.
31721238	4	62	theme	corn-soybean	505:516	arg1	diet					523:526	normal corn-soybean (NC) diet	498:526	normal corn-soybean (NC) diet	498:526	A total of 320, 1-day-old male Arbor Acres broiler chicks were randomly assigned into five groups including normal corn-soybean (NC) diet, corn starch (CS) diet group, 4%, 8% and 12% resistant starch (RS) diet groups.
31721238	6	63	theme	Sellimonas	819:828	arg1	abundances					767:776	the abundances	763:776	the abundances of Bilophila, Eggerthella, Olsenella and Sellimonas	763:828	RESULTS The CS group increased the abundances of Bilophila, Eggerthella, Olsenella and Sellimonas and decreased proportion of Akkermansia, Eisenbergiella, Oscillospira, Ruminococcaceae NK4A214 group and Synergistes in the caecum of birds compared to the NC group.
31721238	3	64	theme	corn	295:298	arg1	starch					300:305	dietary corn starch	287:305	dietary corn starch	287:305	This study investigated the effects of dietary corn starch and resistant starch on composition and function of caecal microbiota of broilers.
31721238	4	65	theme	diet	595:598	arg1	groups					600:605	12% resistant starch (RS) diet groups	569:605	12% resistant starch (RS) diet groups	569:605	A total of 320, 1-day-old male Arbor Acres broiler chicks were randomly assigned into five groups including normal corn-soybean (NC) diet, corn starch (CS) diet group, 4%, 8% and 12% resistant starch (RS) diet groups.
31721238	4	65	theme	diet	595:598	arg1	groups					481:486	five groups	476:486	five groups including normal corn-soybean (NC) diet	476:526	A total of 320, 1-day-old male Arbor Acres broiler chicks were randomly assigned into five groups including normal corn-soybean (NC) diet, corn starch (CS) diet group, 4%, 8% and 12% resistant starch (RS) diet groups.
31721238	7	66	theme	higher	1034:1039	arg1	abundances					1041:1050	higher abundances	1034:1050	higher abundances of Anaerofilum, Bacteroides, Desulfovibrio and Parasutterella	1034:1112	However, the birds from RS groups had higher abundances of Anaerofilum, Bacteroides, Desulfovibrio and Parasutterella and lower abundances of Alistipes, Bilophila, Christensenellaceae R-7 group, Eggerthella and Ruminiclostridium 1 than the CS group.
31721238	1	67	theme	important	122:130	arg1	role					132:135	an important role	119:135	an important role	119:135	BACKGROUND Diet plays an important role in shaping the composition of gut microbiota.
31721238	4	68	theme	1-day-old	406:414	arg1	chicks					441:446	320, 1-day-old male Arbor Acres broiler chicks	401:446	chicks	441:446	A total of 320, 1-day-old male Arbor Acres broiler chicks were randomly assigned into five groups including normal corn-soybean (NC) diet, corn starch (CS) diet group, 4%, 8% and 12% resistant starch (RS) diet groups.
31721238	5	69	theme	old	638:640	arg1	broilers					642:649	42-day old broilers	631:649	42-day old broilers	631:649	The caecal contents of 42-day old broilers were sampled and microbiota community was analysed with 16S rRNA gene sequences.
31721238	6	70	theme	NK4A214	917:923	arg1	group					925:929	Akkermansia, Eisenbergiella, Oscillospira, Ruminococcaceae NK4A214 group	858:929	Akkermansia, Eisenbergiella, Oscillospira, Ruminococcaceae NK4A214 group	858:929	RESULTS The CS group increased the abundances of Bilophila, Eggerthella, Olsenella and Sellimonas and decreased proportion of Akkermansia, Eisenbergiella, Oscillospira, Ruminococcaceae NK4A214 group and Synergistes in the caecum of birds compared to the NC group.
31721238	3	71	theme	starch	321:326	arg1	effects					276:282	the effects	272:282	the effects of dietary corn starch and resistant starch on composition and function of caecal microbiota of broilers	272:387	This study investigated the effects of dietary corn starch and resistant starch on composition and function of caecal microbiota of broilers.
31721238	4	72	theme	starch	583:588	arg1	groups					600:605	12% resistant starch (RS) diet groups	569:605	12% resistant starch (RS) diet groups	569:605	A total of 320, 1-day-old male Arbor Acres broiler chicks were randomly assigned into five groups including normal corn-soybean (NC) diet, corn starch (CS) diet group, 4%, 8% and 12% resistant starch (RS) diet groups.
31721238	4	72	theme	starch	583:588	arg1	groups					481:486	five groups	476:486	five groups including normal corn-soybean (NC) diet	476:526	A total of 320, 1-day-old male Arbor Acres broiler chicks were randomly assigned into five groups including normal corn-soybean (NC) diet, corn starch (CS) diet group, 4%, 8% and 12% resistant starch (RS) diet groups.
31721238	7	73	theme	Desulfovibrio	1081:1093	arg1	abundances					1041:1050	higher abundances	1034:1050	higher abundances of Anaerofilum, Bacteroides, Desulfovibrio and Parasutterella	1034:1112	However, the birds from RS groups had higher abundances of Anaerofilum, Bacteroides, Desulfovibrio and Parasutterella and lower abundances of Alistipes, Bilophila, Christensenellaceae R-7 group, Eggerthella and Ruminiclostridium 1 than the CS group.
31721238	7	73	theme	Desulfovibrio	1081:1093	arg1	abundances					1124:1133	lower abundances	1118:1133	lower abundances of Alistipes, Bilophila, Christensenellaceae R-7 group, Eggerthella and Ruminiclostridium 1	1118:1225	However, the birds from RS groups had higher abundances of Anaerofilum, Bacteroides, Desulfovibrio and Parasutterella and lower abundances of Alistipes, Bilophila, Christensenellaceae R-7 group, Eggerthella and Ruminiclostridium 1 than the CS group.
31721238	7	74	from	groups	1023:1028	arg1	birds					1009:1013	the birds	1005:1013	the birds from RS groups	1005:1028	However, the birds from RS groups had higher abundances of Anaerofilum, Bacteroides, Desulfovibrio and Parasutterella and lower abundances of Alistipes, Bilophila, Christensenellaceae R-7 group, Eggerthella and Ruminiclostridium 1 than the CS group.
31721238	5	75	theme	caecal	612:617	arg1	contents					619:626	The caecal contents	608:626	The caecal contents of 42-day old broilers	608:649	The caecal contents of 42-day old broilers were sampled and microbiota community was analysed with 16S rRNA gene sequences.
31721238	2	76	from	source	202:207	arg1	diets					229:233	diets	229:233	diets of broilers	229:245	Starch is the main source of carbohydrates in diets of broilers.
31721238	8	77	theme	8	1455:1455	arg1	%					1456:1456	%	1456:1456	%	1456:1456	Functional prediction of these changes in microbiota revealed that the CS diet drove caecal microbiota that were more inclined to utilize carbohydrates through glycolysis/gluconeogenesis metabolism, while the 8%RS and 12%RS diets depleted microbial glycolysis/gluconeogenesis and amino acids metabolism.
31721238	4	78	theme	diet	546:549	arg1	group					551:555	corn starch (CS) diet group	529:555	corn starch (CS) diet group	529:555	A total of 320, 1-day-old male Arbor Acres broiler chicks were randomly assigned into five groups including normal corn-soybean (NC) diet, corn starch (CS) diet group, 4%, 8% and 12% resistant starch (RS) diet groups.
31721238	4	78	theme	diet	546:549	arg1	groups					481:486	five groups	476:486	five groups including normal corn-soybean (NC) diet	476:526	A total of 320, 1-day-old male Arbor Acres broiler chicks were randomly assigned into five groups including normal corn-soybean (NC) diet, corn starch (CS) diet group, 4%, 8% and 12% resistant starch (RS) diet groups.
31721238	0	79	theme	resistant	8:16	arg1	starch					18:23	Dietary resistant starch	0:23	Dietary resistant starch	0:23	Dietary resistant starch modifies the composition and function of caecal microbiota of broilers.
31721238	7	80	theme	Ruminiclostridium	1207:1223	arg1	abundances					1041:1050	higher abundances	1034:1050	higher abundances of Anaerofilum, Bacteroides, Desulfovibrio and Parasutterella	1034:1112	However, the birds from RS groups had higher abundances of Anaerofilum, Bacteroides, Desulfovibrio and Parasutterella and lower abundances of Alistipes, Bilophila, Christensenellaceae R-7 group, Eggerthella and Ruminiclostridium 1 than the CS group.
31721238	7	80	theme	Ruminiclostridium	1207:1223	arg1	abundances					1124:1133	lower abundances	1118:1133	lower abundances of Alistipes, Bilophila, Christensenellaceae R-7 group, Eggerthella and Ruminiclostridium 1	1118:1225	However, the birds from RS groups had higher abundances of Anaerofilum, Bacteroides, Desulfovibrio and Parasutterella and lower abundances of Alistipes, Bilophila, Christensenellaceae R-7 group, Eggerthella and Ruminiclostridium 1 than the CS group.
31721238	8	81	theme	RS	1457:1458	arg1	diets					1470:1474	the 8%RS and 12%RS diets	1451:1474	the 8%RS and 12%RS diets	1451:1474	Functional prediction of these changes in microbiota revealed that the CS diet drove caecal microbiota that were more inclined to utilize carbohydrates through glycolysis/gluconeogenesis metabolism, while the 8%RS and 12%RS diets depleted microbial glycolysis/gluconeogenesis and amino acids metabolism.
31721238	4	82	theme	male	416:419	arg1	chicks					441:446	320, 1-day-old male Arbor Acres broiler chicks	401:446	chicks	441:446	A total of 320, 1-day-old male Arbor Acres broiler chicks were randomly assigned into five groups including normal corn-soybean (NC) diet, corn starch (CS) diet group, 4%, 8% and 12% resistant starch (RS) diet groups.
31721238	5	83	theme	16S	707:709	arg1	sequences					721:729	16S rRNA gene sequences	707:729	16S rRNA gene sequences	707:729	The caecal contents of 42-day old broilers were sampled and microbiota community was analysed with 16S rRNA gene sequences.
31721238	1	84	theme	BACKGROUND	97:106	arg1	Diet					108:111	BACKGROUND Diet	97:111	BACKGROUND Diet	97:111	BACKGROUND Diet plays an important role in shaping the composition of gut microbiota.
31721238	7	85	theme	Eggerthella	1191:1201	arg1	abundances					1041:1050	higher abundances	1034:1050	higher abundances of Anaerofilum, Bacteroides, Desulfovibrio and Parasutterella	1034:1112	However, the birds from RS groups had higher abundances of Anaerofilum, Bacteroides, Desulfovibrio and Parasutterella and lower abundances of Alistipes, Bilophila, Christensenellaceae R-7 group, Eggerthella and Ruminiclostridium 1 than the CS group.
31721238	7	85	theme	Eggerthella	1191:1201	arg1	abundances					1124:1133	lower abundances	1118:1133	lower abundances of Alistipes, Bilophila, Christensenellaceae R-7 group, Eggerthella and Ruminiclostridium 1	1118:1225	However, the birds from RS groups had higher abundances of Anaerofilum, Bacteroides, Desulfovibrio and Parasutterella and lower abundances of Alistipes, Bilophila, Christensenellaceae R-7 group, Eggerthella and Ruminiclostridium 1 than the CS group.
31721238	3	86	theme	caecal	359:364	arg1	microbiota					366:375	caecal microbiota	359:375	caecal microbiota of broilers	359:387	This study investigated the effects of dietary corn starch and resistant starch on composition and function of caecal microbiota of broilers.
31721238	6	87	dep	RESULTS	732:738	arg1	decreased					834:842	decreased	834:842	decreased proportion of Akkermansia, Eisenbergiella, Oscillospira, Ruminococcaceae NK4A214 group and Synergistes in the caecum of birds	834:968	RESULTS The CS group increased the abundances of Bilophila, Eggerthella, Olsenella and Sellimonas and decreased proportion of Akkermansia, Eisenbergiella, Oscillospira, Ruminococcaceae NK4A214 group and Synergistes in the caecum of birds compared to the NC group.
31721238	6	87	dep	RESULTS	732:738	arg1	increased					753:761	increased	753:761	increased the abundances of Bilophila, Eggerthella, Olsenella and Sellimonas	753:828	RESULTS The CS group increased the abundances of Bilophila, Eggerthella, Olsenella and Sellimonas and decreased proportion of Akkermansia, Eisenbergiella, Oscillospira, Ruminococcaceae NK4A214 group and Synergistes in the caecum of birds compared to the NC group.
31721238	8	88	theme	RS	1467:1468	arg1	diets					1470:1474	the 8%RS and 12%RS diets	1451:1474	the 8%RS and 12%RS diets	1451:1474	Functional prediction of these changes in microbiota revealed that the CS diet drove caecal microbiota that were more inclined to utilize carbohydrates through glycolysis/gluconeogenesis metabolism, while the 8%RS and 12%RS diets depleted microbial glycolysis/gluconeogenesis and amino acids metabolism.
31721238	5	89	theme	42-day	631:636	arg1	broilers					642:649	42-day old broilers	631:649	42-day old broilers	631:649	The caecal contents of 42-day old broilers were sampled and microbiota community was analysed with 16S rRNA gene sequences.
31721238	0	90	dep	composition	38:48	arg1	the					34:36	the	34:36	the	34:36	Dietary resistant starch modifies the composition and function of caecal microbiota of broilers.
31721238	9	91	theme	hindgut	1759:1765	arg1	health					1767:1772	hindgut health	1759:1772	hindgut health	1759:1772	CONCLUSION Dietary CS and RS alter the microbial composition and diversity, and modulate the metabolic pathways of microbial metabolism in caecum of broilers, which may further affect nutrient utilization and hindgut health of the host.
31721238	4	92	theme	NC	519:520	arg1	diet					523:526	normal corn-soybean (NC) diet	498:526	normal corn-soybean (NC) diet	498:526	A total of 320, 1-day-old male Arbor Acres broiler chicks were randomly assigned into five groups including normal corn-soybean (NC) diet, corn starch (CS) diet group, 4%, 8% and 12% resistant starch (RS) diet groups.
31721238	2	93	theme	main	197:200	arg1	source					202:207	the main source	193:207	the main source of carbohydrates in diets of broilers	193:245	Starch is the main source of carbohydrates in diets of broilers.
31721238	2	93	theme	main	197:200	arg1	Starch					183:188	Starch	183:188	Starch	183:188	Starch is the main source of carbohydrates in diets of broilers.
31721238	7	94	theme	Bilophila	1149:1157	arg1	abundances					1041:1050	higher abundances	1034:1050	higher abundances of Anaerofilum, Bacteroides, Desulfovibrio and Parasutterella	1034:1112	However, the birds from RS groups had higher abundances of Anaerofilum, Bacteroides, Desulfovibrio and Parasutterella and lower abundances of Alistipes, Bilophila, Christensenellaceae R-7 group, Eggerthella and Ruminiclostridium 1 than the CS group.
31721238	7	94	theme	Bilophila	1149:1157	arg1	abundances					1124:1133	lower abundances	1118:1133	lower abundances of Alistipes, Bilophila, Christensenellaceae R-7 group, Eggerthella and Ruminiclostridium 1	1118:1225	However, the birds from RS groups had higher abundances of Anaerofilum, Bacteroides, Desulfovibrio and Parasutterella and lower abundances of Alistipes, Bilophila, Christensenellaceae R-7 group, Eggerthella and Ruminiclostridium 1 than the CS group.
31721238	7	95	theme	Alistipes	1138:1146	arg1	abundances					1041:1050	higher abundances	1034:1050	higher abundances of Anaerofilum, Bacteroides, Desulfovibrio and Parasutterella	1034:1112	However, the birds from RS groups had higher abundances of Anaerofilum, Bacteroides, Desulfovibrio and Parasutterella and lower abundances of Alistipes, Bilophila, Christensenellaceae R-7 group, Eggerthella and Ruminiclostridium 1 than the CS group.
31721238	7	95	theme	Alistipes	1138:1146	arg1	abundances					1124:1133	lower abundances	1118:1133	lower abundances of Alistipes, Bilophila, Christensenellaceae R-7 group, Eggerthella and Ruminiclostridium 1	1118:1225	However, the birds from RS groups had higher abundances of Anaerofilum, Bacteroides, Desulfovibrio and Parasutterella and lower abundances of Alistipes, Bilophila, Christensenellaceae R-7 group, Eggerthella and Ruminiclostridium 1 than the CS group.
31721238	9	96	theme	host	1781:1784	arg1	utilization					1743:1753	nutrient utilization	1734:1753	nutrient utilization	1734:1753	CONCLUSION Dietary CS and RS alter the microbial composition and diversity, and modulate the metabolic pathways of microbial metabolism in caecum of broilers, which may further affect nutrient utilization and hindgut health of the host.
31721238	9	96	theme	host	1781:1784	arg1	health					1767:1772	hindgut health	1759:1772	hindgut health	1759:1772	CONCLUSION Dietary CS and RS alter the microbial composition and diversity, and modulate the metabolic pathways of microbial metabolism in caecum of broilers, which may further affect nutrient utilization and hindgut health of the host.
31721238	8	97	theme	amino	1526:1530	arg1	acids					1532:1536	amino acids	1526:1536	amino acids	1526:1536	Functional prediction of these changes in microbiota revealed that the CS diet drove caecal microbiota that were more inclined to utilize carbohydrates through glycolysis/gluconeogenesis metabolism, while the 8%RS and 12%RS diets depleted microbial glycolysis/gluconeogenesis and amino acids metabolism.
31721238	8	98	from	changes	1277:1283	arg1	microbiota					1288:1297	microbiota	1288:1297	microbiota	1288:1297	Functional prediction of these changes in microbiota revealed that the CS diet drove caecal microbiota that were more inclined to utilize carbohydrates through glycolysis/gluconeogenesis metabolism, while the 8%RS and 12%RS diets depleted microbial glycolysis/gluconeogenesis and amino acids metabolism.
30792412	5	0	theme	at	869:870	arg1	ratio					878:882	at a 9:1 ratio	869:882	at a 9:1 ratio; total 0.6 g/100 ml	869:902	We showed that today's formulas supplemented with either scGOS (0.24-0.50 g/100 ml) or scGOS and lcFOS (at a 9:1 ratio; total 0.6 g/100 ml) had a strong bifidogenic effect as compared to traditional formulas, and they also resulted in altered patterns of microbial colonisation within the developing infant gastrointestinal tract.
30792412	5	1	theme	9:1	874:876	arg1	ratio					878:882	at a 9:1 ratio	869:882	at a 9:1 ratio; total 0.6 g/100 ml	869:902	We showed that today's formulas supplemented with either scGOS (0.24-0.50 g/100 ml) or scGOS and lcFOS (at a 9:1 ratio; total 0.6 g/100 ml) had a strong bifidogenic effect as compared to traditional formulas, and they also resulted in altered patterns of microbial colonisation within the developing infant gastrointestinal tract.
30792412	0	2	from	effect	4:9	arg1	dynamics					80:87	dynamics	80:87	dynamics	80:87	The effect of prebiotic fortified infant formulas on microbiota composition and dynamics in early life.
30792412	0	2	from	effect	4:9	arg1	composition					64:74	microbiota composition	53:74	microbiota composition	53:74	The effect of prebiotic fortified infant formulas on microbiota composition and dynamics in early life.
30792412	6	3	theme	dominated	1249:1257	arg1	state					1259:1263	a bifidobacteria dominated state	1232:1263	a bifidobacteria dominated state	1232:1263	We identified three microbial states (or developmental stages) in the first 12 weeks of life, with a gradual transition pattern towards a bifidobacteria dominated state.
30792412	2	4	from	effect	321:326	arg1	composition					345:355	GI microbiota composition	331:355	GI microbiota composition	331:355	Today's infant formulas are often fortified with prebiotics to better mimic properties of human milk with respect to its effect on GI microbiota composition and function.
30792412	2	4	from	effect	321:326	arg1	function					361:368	function	361:368	function	361:368	Today's infant formulas are often fortified with prebiotics to better mimic properties of human milk with respect to its effect on GI microbiota composition and function.
30792412	2	5	theme	microbiota	334:343	arg1	composition					345:355	GI microbiota composition	331:355	GI microbiota composition	331:355	Today's infant formulas are often fortified with prebiotics to better mimic properties of human milk with respect to its effect on GI microbiota composition and function.
30792412	5	6	theme	colonisation	1030:1041	arg1	patterns					1008:1015	altered patterns	1000:1015	altered patterns of microbial colonisation within the developing infant gastrointestinal tract	1000:1093	We showed that today's formulas supplemented with either scGOS (0.24-0.50 g/100 ml) or scGOS and lcFOS (at a 9:1 ratio; total 0.6 g/100 ml) had a strong bifidogenic effect as compared to traditional formulas, and they also resulted in altered patterns of microbial colonisation within the developing infant gastrointestinal tract.
30792412	0	7	theme	early	92:96	arg1	life					98:101	early life	92:101	early life	92:101	The effect of prebiotic fortified infant formulas on microbiota composition and dynamics in early life.
30792412	2	8	theme	human	290:294	arg1	milk					296:299	human milk	290:299	human milk	290:299	Today's infant formulas are often fortified with prebiotics to better mimic properties of human milk with respect to its effect on GI microbiota composition and function.
30792412	5	9	theme	bifidogenic	918:928	arg1	effect					930:935	a strong bifidogenic effect	909:935	a strong bifidogenic effect	909:935	We showed that today's formulas supplemented with either scGOS (0.24-0.50 g/100 ml) or scGOS and lcFOS (at a 9:1 ratio; total 0.6 g/100 ml) had a strong bifidogenic effect as compared to traditional formulas, and they also resulted in altered patterns of microbial colonisation within the developing infant gastrointestinal tract.
30792412	6	10	theme	developmental	1137:1149	arg1	stages					1151:1156	developmental stages	1137:1156	developmental stages	1137:1156	We identified three microbial states (or developmental stages) in the first 12 weeks of life, with a gradual transition pattern towards a bifidobacteria dominated state.
30792412	5	11	theme	infant	1065:1070	arg1	tract					1089:1093	the developing infant gastrointestinal tract	1050:1093	the developing infant gastrointestinal tract	1050:1093	We showed that today's formulas supplemented with either scGOS (0.24-0.50 g/100 ml) or scGOS and lcFOS (at a 9:1 ratio; total 0.6 g/100 ml) had a strong bifidogenic effect as compared to traditional formulas, and they also resulted in altered patterns of microbial colonisation within the developing infant gastrointestinal tract.
30792412	2	12	theme	GI	331:332	arg1	composition					345:355	GI microbiota composition	331:355	GI microbiota composition	331:355	Today's infant formulas are often fortified with prebiotics to better mimic properties of human milk with respect to its effect on GI microbiota composition and function.
30792412	1	13	dep	Gastrointestinal	104:119	arg1	GI					122:123	GI	122:123	GI	122:123	Gastrointestinal (GI) microbiota composition differs between breastfed and formula-fed infants.
30792412	3	14	theme	16S	422:424	arg1	rRNA					426:429	16S rRNA	422:429	PCR-amplified 16S rRNA gene fragments	408:444	We used Illumina HiSeq sequencing of PCR-amplified 16S rRNA gene fragments to investigate the composition of faecal microbiota in 2-12 week old infants receiving either breastmilk, infant formulas fortified with prebiotics, or mixed feeding.
30792412	1	15	theme	breastfed	165:173	arg1	infants					191:197	breastfed and formula-fed infants	165:197	breastfed and formula-fed infants	165:197	Gastrointestinal (GI) microbiota composition differs between breastfed and formula-fed infants.
30792412	7	16	dep	accelerated	1375:1385	arg1	whereas					1388:1394	whereas	1388:1394	whereas	1388:1394	In infants receiving only fortified formulas, this transition towards the bifidobacteria dominated state was accelerated, whereas in infants receiving mixed feeding the transition was delayed, as compared to exclusively breastfed infants.
30792412	3	17	theme	microbiota	487:496	arg1	composition					465:475	the composition	461:475	the composition of faecal microbiota in 2-12 week old infants receiving either breastmilk, infant formulas fortified with prebiotics, or mixed feeding	461:610	We used Illumina HiSeq sequencing of PCR-amplified 16S rRNA gene fragments to investigate the composition of faecal microbiota in 2-12 week old infants receiving either breastmilk, infant formulas fortified with prebiotics, or mixed feeding.
30792412	3	18	theme	fragments	436:444	arg1	sequencing					394:403	Illumina HiSeq sequencing	379:403	Illumina HiSeq sequencing of PCR-amplified 16S rRNA gene fragments	379:444	We used Illumina HiSeq sequencing of PCR-amplified 16S rRNA gene fragments to investigate the composition of faecal microbiota in 2-12 week old infants receiving either breastmilk, infant formulas fortified with prebiotics, or mixed feeding.
30792412	6	19	theme	transition	1205:1214	arg1	pattern					1216:1222	a gradual transition pattern	1195:1222	a gradual transition pattern towards a bifidobacteria dominated state	1195:1263	We identified three microbial states (or developmental stages) in the first 12 weeks of life, with a gradual transition pattern towards a bifidobacteria dominated state.
30792412	5	20	theme	strong	911:916	arg1	effect					930:935	a strong bifidogenic effect	909:935	a strong bifidogenic effect	909:935	We showed that today's formulas supplemented with either scGOS (0.24-0.50 g/100 ml) or scGOS and lcFOS (at a 9:1 ratio; total 0.6 g/100 ml) had a strong bifidogenic effect as compared to traditional formulas, and they also resulted in altered patterns of microbial colonisation within the developing infant gastrointestinal tract.
30792412	5	21	theme	gastrointestinal	1072:1087	arg1	tract					1089:1093	the developing infant gastrointestinal tract	1050:1093	the developing infant gastrointestinal tract	1050:1093	We showed that today's formulas supplemented with either scGOS (0.24-0.50 g/100 ml) or scGOS and lcFOS (at a 9:1 ratio; total 0.6 g/100 ml) had a strong bifidogenic effect as compared to traditional formulas, and they also resulted in altered patterns of microbial colonisation within the developing infant gastrointestinal tract.
30792412	1	22	theme	formula-fed	179:189	arg1	infants					191:197	breastfed and formula-fed infants	165:197	breastfed and formula-fed infants	165:197	Gastrointestinal (GI) microbiota composition differs between breastfed and formula-fed infants.
30792412	3	23	theme	Illumina	379:386	arg1	sequencing					394:403	Illumina HiSeq sequencing	379:403	Illumina HiSeq sequencing of PCR-amplified 16S rRNA gene fragments	379:444	We used Illumina HiSeq sequencing of PCR-amplified 16S rRNA gene fragments to investigate the composition of faecal microbiota in 2-12 week old infants receiving either breastmilk, infant formulas fortified with prebiotics, or mixed feeding.
30792412	6	24	theme	gradual	1197:1203	arg1	pattern					1216:1222	a gradual transition pattern	1195:1222	a gradual transition pattern towards a bifidobacteria dominated state	1195:1263	We identified three microbial states (or developmental stages) in the first 12 weeks of life, with a gradual transition pattern towards a bifidobacteria dominated state.
30792412	5	25	theme	altered	1000:1006	arg1	patterns					1008:1015	altered patterns	1000:1015	altered patterns of microbial colonisation within the developing infant gastrointestinal tract	1000:1093	We showed that today's formulas supplemented with either scGOS (0.24-0.50 g/100 ml) or scGOS and lcFOS (at a 9:1 ratio; total 0.6 g/100 ml) had a strong bifidogenic effect as compared to traditional formulas, and they also resulted in altered patterns of microbial colonisation within the developing infant gastrointestinal tract.
30792412	3	26	theme	rRNA	426:429	arg1	fragments					436:444	PCR-amplified 16S rRNA gene fragments	408:444	PCR-amplified 16S rRNA gene fragments	408:444	We used Illumina HiSeq sequencing of PCR-amplified 16S rRNA gene fragments to investigate the composition of faecal microbiota in 2-12 week old infants receiving either breastmilk, infant formulas fortified with prebiotics, or mixed feeding.
30792412	0	27	theme	prebiotic	14:22	arg1	formulas					41:48	prebiotic fortified infant formulas	14:48	prebiotic fortified infant formulas	14:48	The effect of prebiotic fortified infant formulas on microbiota composition and dynamics in early life.
30792412	5	28	theme	developing	1054:1063	arg1	tract					1089:1093	the developing infant gastrointestinal tract	1050:1093	the developing infant gastrointestinal tract	1050:1093	We showed that today's formulas supplemented with either scGOS (0.24-0.50 g/100 ml) or scGOS and lcFOS (at a 9:1 ratio; total 0.6 g/100 ml) had a strong bifidogenic effect as compared to traditional formulas, and they also resulted in altered patterns of microbial colonisation within the developing infant gastrointestinal tract.
30792412	7	29	theme	dominated	1355:1363	arg1	state					1365:1369	the bifidobacteria dominated state	1336:1369	the bifidobacteria dominated state	1336:1369	In infants receiving only fortified formulas, this transition towards the bifidobacteria dominated state was accelerated, whereas in infants receiving mixed feeding the transition was delayed, as compared to exclusively breastfed infants.
30792412	3	30	theme	infant	552:557	arg1	breastmilk					540:549	breastmilk	540:549	breastmilk	540:549	We used Illumina HiSeq sequencing of PCR-amplified 16S rRNA gene fragments to investigate the composition of faecal microbiota in 2-12 week old infants receiving either breastmilk, infant formulas fortified with prebiotics, or mixed feeding.
30792412	3	30	theme	infant	552:557	arg1	formulas					559:566	infant formulas	552:566	infant formulas fortified with prebiotics	552:592	We used Illumina HiSeq sequencing of PCR-amplified 16S rRNA gene fragments to investigate the composition of faecal microbiota in 2-12 week old infants receiving either breastmilk, infant formulas fortified with prebiotics, or mixed feeding.
30792412	6	31	from	states	1126:1131	arg1	weeks					1175:1179	the first 12 weeks	1162:1179	the first 12 weeks of life	1162:1187	We identified three microbial states (or developmental stages) in the first 12 weeks of life, with a gradual transition pattern towards a bifidobacteria dominated state.
30792412	7	32	theme	only	1287:1290	arg1	formulas					1302:1309	only fortified formulas	1287:1309	only fortified formulas	1287:1309	In infants receiving only fortified formulas, this transition towards the bifidobacteria dominated state was accelerated, whereas in infants receiving mixed feeding the transition was delayed, as compared to exclusively breastfed infants.
30792412	5	33	dep	ratio	878:882	arg1	0.6 g/100 ml					891:902	total 0.6 g/100 ml	885:902	at a 9:1 ratio; total 0.6 g/100 ml	869:902	We showed that today's formulas supplemented with either scGOS (0.24-0.50 g/100 ml) or scGOS and lcFOS (at a 9:1 ratio; total 0.6 g/100 ml) had a strong bifidogenic effect as compared to traditional formulas, and they also resulted in altered patterns of microbial colonisation within the developing infant gastrointestinal tract.
30792412	0	34	from	dynamics	80:87	arg1	life					98:101	early life	92:101	early life	92:101	The effect of prebiotic fortified infant formulas on microbiota composition and dynamics in early life.
30792412	2	35	theme	milk	296:299	arg1	properties					276:285	properties	276:285	properties of human milk	276:299	Today's infant formulas are often fortified with prebiotics to better mimic properties of human milk with respect to its effect on GI microbiota composition and function.
30792412	3	36	theme	old	511:513	arg1	infants					515:521	2-12 week old infants	501:521	2-12 week old infants receiving either breastmilk, infant formulas fortified with prebiotics, or mixed feeding	501:610	We used Illumina HiSeq sequencing of PCR-amplified 16S rRNA gene fragments to investigate the composition of faecal microbiota in 2-12 week old infants receiving either breastmilk, infant formulas fortified with prebiotics, or mixed feeding.
30792412	0	37	theme	infant	34:39	arg1	formulas					41:48	prebiotic fortified infant formulas	14:48	prebiotic fortified infant formulas	14:48	The effect of prebiotic fortified infant formulas on microbiota composition and dynamics in early life.
30792412	4	38	contain	contained	734:742	arg1	2002-2003					717:725	2002-2003	717:725	2002-2003	717:725	We compared these results with results from infants fed traditional formulas used in the Netherlands in 2002-2003, which contained no added prebiotics.
30792412	4	38	contain	contained	734:742	arg2	prebiotics					753:762	no added prebiotics	744:762	no added prebiotics	744:762	We compared these results with results from infants fed traditional formulas used in the Netherlands in 2002-2003, which contained no added prebiotics.
30792412	5	39	contain	had	905:907	arg1	formulas					788:795	today's formulas	780:795	today's formulas supplemented with either scGOS (0.24-0.50 g/100 ml) or scGOS and lcFOS (at a 9:1 ratio; total 0.6 g/100 ml)	780:903	We showed that today's formulas supplemented with either scGOS (0.24-0.50 g/100 ml) or scGOS and lcFOS (at a 9:1 ratio; total 0.6 g/100 ml) had a strong bifidogenic effect as compared to traditional formulas, and they also resulted in altered patterns of microbial colonisation within the developing infant gastrointestinal tract.
30792412	5	39	contain	had	905:907	arg2	effect					930:935	a strong bifidogenic effect	909:935	a strong bifidogenic effect	909:935	We showed that today's formulas supplemented with either scGOS (0.24-0.50 g/100 ml) or scGOS and lcFOS (at a 9:1 ratio; total 0.6 g/100 ml) had a strong bifidogenic effect as compared to traditional formulas, and they also resulted in altered patterns of microbial colonisation within the developing infant gastrointestinal tract.
30792412	0	40	theme	fortified	24:32	arg1	formulas					41:48	prebiotic fortified infant formulas	14:48	prebiotic fortified infant formulas	14:48	The effect of prebiotic fortified infant formulas on microbiota composition and dynamics in early life.
30792412	3	41	used	used	374:377	arg2	We					371:372	We	371:372	We	371:372	We used Illumina HiSeq sequencing of PCR-amplified 16S rRNA gene fragments to investigate the composition of faecal microbiota in 2-12 week old infants receiving either breastmilk, infant formulas fortified with prebiotics, or mixed feeding.
30792412	0	42	from	composition	64:74	arg1	life					98:101	early life	92:101	early life	92:101	The effect of prebiotic fortified infant formulas on microbiota composition and dynamics in early life.
30792412	3	43	theme	mixed	598:602	arg1	feeding					604:610	mixed feeding	598:610	mixed feeding	598:610	We used Illumina HiSeq sequencing of PCR-amplified 16S rRNA gene fragments to investigate the composition of faecal microbiota in 2-12 week old infants receiving either breastmilk, infant formulas fortified with prebiotics, or mixed feeding.
30792412	3	44	theme	HiSeq	388:392	arg1	sequencing					394:403	Illumina HiSeq sequencing	379:403	Illumina HiSeq sequencing of PCR-amplified 16S rRNA gene fragments	379:444	We used Illumina HiSeq sequencing of PCR-amplified 16S rRNA gene fragments to investigate the composition of faecal microbiota in 2-12 week old infants receiving either breastmilk, infant formulas fortified with prebiotics, or mixed feeding.
30792412	4	45	theme	traditional	669:679	arg1	formulas					681:688	traditional formulas	669:688	traditional formulas used in the Netherlands in 2002-2003, which contained no added prebiotics	669:762	We compared these results with results from infants fed traditional formulas used in the Netherlands in 2002-2003, which contained no added prebiotics.
30792412	3	46	theme	gene	431:434	arg1	fragments					436:444	PCR-amplified 16S rRNA gene fragments	408:444	PCR-amplified 16S rRNA gene fragments	408:444	We used Illumina HiSeq sequencing of PCR-amplified 16S rRNA gene fragments to investigate the composition of faecal microbiota in 2-12 week old infants receiving either breastmilk, infant formulas fortified with prebiotics, or mixed feeding.
30792412	5	47	dep	scGOS	852:856	arg1	ratio					878:882	at a 9:1 ratio	869:882	at a 9:1 ratio; total 0.6 g/100 ml	869:902	We showed that today's formulas supplemented with either scGOS (0.24-0.50 g/100 ml) or scGOS and lcFOS (at a 9:1 ratio; total 0.6 g/100 ml) had a strong bifidogenic effect as compared to traditional formulas, and they also resulted in altered patterns of microbial colonisation within the developing infant gastrointestinal tract.
30792412	6	48	from	stages	1151:1156	arg1	weeks					1175:1179	the first 12 weeks	1162:1179	the first 12 weeks of life	1162:1187	We identified three microbial states (or developmental stages) in the first 12 weeks of life, with a gradual transition pattern towards a bifidobacteria dominated state.
30792412	1	49	theme	Gastrointestinal	104:119	arg1	composition					137:147	Gastrointestinal (GI) microbiota composition	104:147	Gastrointestinal (GI) microbiota composition	104:147	Gastrointestinal (GI) microbiota composition differs between breastfed and formula-fed infants.
30792412	0	50	theme	formulas	41:48	arg1	effect					4:9	The effect	0:9	The effect of prebiotic fortified infant formulas on microbiota composition and dynamics in early life.	0:102	The effect of prebiotic fortified infant formulas on microbiota composition and dynamics in early life.
30792412	7	51	theme	fortified	1292:1300	arg1	formulas					1302:1309	only fortified formulas	1287:1309	only fortified formulas	1287:1309	In infants receiving only fortified formulas, this transition towards the bifidobacteria dominated state was accelerated, whereas in infants receiving mixed feeding the transition was delayed, as compared to exclusively breastfed infants.
30792412	2	52	theme	infant	208:213	arg1	formulas					215:222	Today's infant formulas	200:222	Today's infant formulas	200:222	Today's infant formulas are often fortified with prebiotics to better mimic properties of human milk with respect to its effect on GI microbiota composition and function.
30792412	6	53	theme	life	1184:1187	arg1	weeks					1175:1179	the first 12 weeks	1162:1179	the first 12 weeks of life	1162:1187	We identified three microbial states (or developmental stages) in the first 12 weeks of life, with a gradual transition pattern towards a bifidobacteria dominated state.
30792412	5	54	theme	total	885:889	arg1	0.6 g/100 ml					891:902	total 0.6 g/100 ml	885:902	at a 9:1 ratio; total 0.6 g/100 ml	869:902	We showed that today's formulas supplemented with either scGOS (0.24-0.50 g/100 ml) or scGOS and lcFOS (at a 9:1 ratio; total 0.6 g/100 ml) had a strong bifidogenic effect as compared to traditional formulas, and they also resulted in altered patterns of microbial colonisation within the developing infant gastrointestinal tract.
30792412	5	55	theme	traditional	952:962	arg1	formulas					964:971	traditional formulas	952:971	traditional formulas	952:971	We showed that today's formulas supplemented with either scGOS (0.24-0.50 g/100 ml) or scGOS and lcFOS (at a 9:1 ratio; total 0.6 g/100 ml) had a strong bifidogenic effect as compared to traditional formulas, and they also resulted in altered patterns of microbial colonisation within the developing infant gastrointestinal tract.
30792412	3	56	from	composition	465:475	arg1	infants					515:521	2-12 week old infants	501:521	2-12 week old infants receiving either breastmilk, infant formulas fortified with prebiotics, or mixed feeding	501:610	We used Illumina HiSeq sequencing of PCR-amplified 16S rRNA gene fragments to investigate the composition of faecal microbiota in 2-12 week old infants receiving either breastmilk, infant formulas fortified with prebiotics, or mixed feeding.
30792412	4	57	theme	added	747:751	arg1	prebiotics					753:762	no added prebiotics	744:762	no added prebiotics	744:762	We compared these results with results from infants fed traditional formulas used in the Netherlands in 2002-2003, which contained no added prebiotics.
30792412	7	58	theme	mixed	1417:1421	arg1	feeding					1423:1429	mixed feeding	1417:1429	mixed feeding	1417:1429	In infants receiving only fortified formulas, this transition towards the bifidobacteria dominated state was accelerated, whereas in infants receiving mixed feeding the transition was delayed, as compared to exclusively breastfed infants.
30792412	3	59	theme	faecal	480:485	arg1	microbiota					487:496	faecal microbiota	480:496	faecal microbiota	480:496	We used Illumina HiSeq sequencing of PCR-amplified 16S rRNA gene fragments to investigate the composition of faecal microbiota in 2-12 week old infants receiving either breastmilk, infant formulas fortified with prebiotics, or mixed feeding.
30792412	6	60	theme	microbial	1116:1124	arg1	states					1126:1131	three microbial states	1110:1131	three microbial states	1110:1131	We identified three microbial states (or developmental stages) in the first 12 weeks of life, with a gradual transition pattern towards a bifidobacteria dominated state.
30792412	4	61	from	infants	657:663	arg1	results					644:650	results	644:650	results from infants fed traditional formulas used in the Netherlands in 2002-2003, which contained no added prebiotics	644:762	We compared these results with results from infants fed traditional formulas used in the Netherlands in 2002-2003, which contained no added prebiotics.
30792412	5	62	theme	microbial	1020:1028	arg1	colonisation					1030:1041	microbial colonisation	1020:1041	microbial colonisation within the developing infant gastrointestinal tract	1020:1093	We showed that today's formulas supplemented with either scGOS (0.24-0.50 g/100 ml) or scGOS and lcFOS (at a 9:1 ratio; total 0.6 g/100 ml) had a strong bifidogenic effect as compared to traditional formulas, and they also resulted in altered patterns of microbial colonisation within the developing infant gastrointestinal tract.
30792412	1	63	theme	 microbiota	125:135	arg1	composition					137:147	Gastrointestinal (GI) microbiota composition	104:147	Gastrointestinal (GI) microbiota composition	104:147	Gastrointestinal (GI) microbiota composition differs between breastfed and formula-fed infants.
30792412	0	64	theme	microbiota	53:62	arg1	composition					64:74	microbiota composition	53:74	microbiota composition	53:74	The effect of prebiotic fortified infant formulas on microbiota composition and dynamics in early life.
30792412	3	65	theme	PCR-amplified	408:420	arg1	fragments					436:444	PCR-amplified 16S rRNA gene fragments	408:444	PCR-amplified 16S rRNA gene fragments	408:444	We used Illumina HiSeq sequencing of PCR-amplified 16S rRNA gene fragments to investigate the composition of faecal microbiota in 2-12 week old infants receiving either breastmilk, infant formulas fortified with prebiotics, or mixed feeding.
30792412	6	66	theme	first	1166:1170	arg1	weeks					1175:1179	the first 12 weeks	1162:1179	the first 12 weeks of life	1162:1187	We identified three microbial states (or developmental stages) in the first 12 weeks of life, with a gradual transition pattern towards a bifidobacteria dominated state.
30792412	7	67	theme	breastfed	1486:1494	arg1	infants					1496:1502	exclusively breastfed infants	1474:1502	exclusively breastfed infants	1474:1502	In infants receiving only fortified formulas, this transition towards the bifidobacteria dominated state was accelerated, whereas in infants receiving mixed feeding the transition was delayed, as compared to exclusively breastfed infants.
31183067	1	0	attach	present	177:183	arg1	glycoproteins					195:207	target glycoproteins	188:207	target glycoproteins	188:207	Lectins are carbohydrate binding proteins that recognize specific epitopes present on target glycoproteins.
31183067	1	0	attach	present	177:183	arg2	epitopes					168:175	specific epitopes	159:175	specific epitopes present on target glycoproteins	159:207	Lectins are carbohydrate binding proteins that recognize specific epitopes present on target glycoproteins.
31183067	8	1	theme	branched	1350:1357	arg1	glycoproteoforms					1359:1374	highly branched glycoproteoforms	1343:1374	highly branched glycoproteoforms of haptoglobin and α1-acid glycoprotein	1343:1414	Secondly, we analyze the global heterogeneity of highly branched glycoproteoforms of haptoglobin and α1-acid glycoprotein and reveal that while multi-fucosylation attenuates the lectin PHA-L binding to haptoglobin, it has no impact on AGP.
31183067	5	2	theme	native	788:793	arg1	spectrometry					800:811	native mass spectrometry	788:811	native mass spectrometry to characterize lectin-reactive glycoproteoforms at the intact protein level	788:888	Here, we apply a lectin-affinity purification procedure coupled with native mass spectrometry to characterize lectin-reactive glycoproteoforms at the intact protein level.
31183067	2	3	theme	pathological	351:362	arg1	processes					364:372	several pathological processes	343:372	several pathological processes	343:372	Changes in lectin-reactive carbohydrate repertoires are related to many biological signaling pathways and recognized as hallmarks of several pathological processes.
31183067	10	4	theme	many	1776:1779	arg1	hepatic					1813:1819	hepatic	1813:1819	hepatic	1813:1819	Moreover, since aberrant glycosylation of Hp and AGP are potential markers for many diseases, including pancreatic, hepatic and ovarian cancers, understanding their interactions with lectins will help the development of carbohydrate-centric monitoring methods to understand their pathophysiological implications.
31183067	10	4	theme	many	1776:1779	arg1	diseases					1781:1788	many diseases	1776:1788	many diseases	1776:1788	Moreover, since aberrant glycosylation of Hp and AGP are potential markers for many diseases, including pancreatic, hepatic and ovarian cancers, understanding their interactions with lectins will help the development of carbohydrate-centric monitoring methods to understand their pathophysiological implications.
31183067	10	4	theme	many	1776:1779	arg1	pancreatic					1801:1810	pancreatic	1801:1810	pancreatic	1801:1810	Moreover, since aberrant glycosylation of Hp and AGP are potential markers for many diseases, including pancreatic, hepatic and ovarian cancers, understanding their interactions with lectins will help the development of carbohydrate-centric monitoring methods to understand their pathophysiological implications.
31183067	10	4	theme	many	1776:1779	arg1	cancers					1833:1839	ovarian cancers	1825:1839	ovarian cancers	1825:1839	Moreover, since aberrant glycosylation of Hp and AGP are potential markers for many diseases, including pancreatic, hepatic and ovarian cancers, understanding their interactions with lectins will help the development of carbohydrate-centric monitoring methods to understand their pathophysiological implications.
31183067	6	5	theme	fucosylated	942:952	arg1	glycoproteoforms					974:989	the highly fucosylated and highly branched glycoproteoforms	931:989	the highly fucosylated and highly branched glycoproteoforms of haptoglobin and α1-acid glycoprotein	931:1029	We investigate the interactions between the highly fucosylated and highly branched glycoproteoforms of haptoglobin and α1-acid glycoprotein using two different lectins Aleuria aurantia lectin (AAL) and Phaseolus vulgaris leucoagglutinin (PHA-L), respectively.
31183067	8	6	theme	lectin	1472:1477	arg1	binding					1485:1491	the lectin PHA-L binding	1468:1491	the lectin PHA-L binding to haptoglobin	1468:1506	Secondly, we analyze the global heterogeneity of highly branched glycoproteoforms of haptoglobin and α1-acid glycoprotein and reveal that while multi-fucosylation attenuates the lectin PHA-L binding to haptoglobin, it has no impact on AGP.
31183067	7	7	theme	fucosylated	1264:1274	arg1	glycoproteoforms					1276:1291	highly fucosylated glycoproteoforms	1257:1291	highly fucosylated glycoproteoforms	1257:1291	Firstly we show a co-occurrence of fucosylation and N-glycan branching on haptoglobin, particularly among highly fucosylated glycoproteoforms.
31183067	8	8	theme	glycoprotein	1403:1414	arg1	glycoproteoforms					1359:1374	highly branched glycoproteoforms	1343:1374	highly branched glycoproteoforms of haptoglobin and α1-acid glycoprotein	1343:1414	Secondly, we analyze the global heterogeneity of highly branched glycoproteoforms of haptoglobin and α1-acid glycoprotein and reveal that while multi-fucosylation attenuates the lectin PHA-L binding to haptoglobin, it has no impact on AGP.
31183067	6	9	gly	fucosylated	942:952	arg1	haptoglobin					994:1004	haptoglobin	994:1004	haptoglobin	994:1004	We investigate the interactions between the highly fucosylated and highly branched glycoproteoforms of haptoglobin and α1-acid glycoprotein using two different lectins Aleuria aurantia lectin (AAL) and Phaseolus vulgaris leucoagglutinin (PHA-L), respectively.
31183067	6	9	gly	fucosylated	942:952	arg1	glycoprotein					1018:1029	α1-acid glycoprotein	1010:1029	α1-acid glycoprotein	1010:1029	We investigate the interactions between the highly fucosylated and highly branched glycoproteoforms of haptoglobin and α1-acid glycoprotein using two different lectins Aleuria aurantia lectin (AAL) and Phaseolus vulgaris leucoagglutinin (PHA-L), respectively.
31183067	6	9	gly	fucosylated	942:952	arg1	glycoproteoforms					974:989	the highly fucosylated and highly branched glycoproteoforms	931:989	the highly fucosylated and highly branched glycoproteoforms of haptoglobin and α1-acid glycoprotein	931:1029	We investigate the interactions between the highly fucosylated and highly branched glycoproteoforms of haptoglobin and α1-acid glycoprotein using two different lectins Aleuria aurantia lectin (AAL) and Phaseolus vulgaris leucoagglutinin (PHA-L), respectively.
31183067	10	10	gly	glycosylation	1722:1734	arg1	Hp					1739:1740	Hp	1739:1740	Hp	1739:1740	Moreover, since aberrant glycosylation of Hp and AGP are potential markers for many diseases, including pancreatic, hepatic and ovarian cancers, understanding their interactions with lectins will help the development of carbohydrate-centric monitoring methods to understand their pathophysiological implications.
31183067	10	10	gly	glycosylation	1722:1734	arg1	AGP					1746:1748	AGP	1746:1748	AGP	1746:1748	Moreover, since aberrant glycosylation of Hp and AGP are potential markers for many diseases, including pancreatic, hepatic and ovarian cancers, understanding their interactions with lectins will help the development of carbohydrate-centric monitoring methods to understand their pathophysiological implications.
31183067	2	11	theme	signaling	293:301	arg1	pathways					303:310	many biological signaling pathways	277:310	many biological signaling pathways	277:310	Changes in lectin-reactive carbohydrate repertoires are related to many biological signaling pathways and recognized as hallmarks of several pathological processes.
31183067	6	12	theme	Phaseolus	1093:1101	arg1	leucoagglutinin					1112:1126	Phaseolus vulgaris leucoagglutinin	1093:1126	Phaseolus vulgaris leucoagglutinin (PHA-L)	1093:1134	We investigate the interactions between the highly fucosylated and highly branched glycoproteoforms of haptoglobin and α1-acid glycoprotein using two different lectins Aleuria aurantia lectin (AAL) and Phaseolus vulgaris leucoagglutinin (PHA-L), respectively.
31183067	6	12	theme	Phaseolus	1093:1101	arg1	lectins					1051:1057	two different lectins	1037:1057	two different lectins Aleuria aurantia lectin (AAL) and Phaseolus vulgaris leucoagglutinin (PHA-L)	1037:1134	We investigate the interactions between the highly fucosylated and highly branched glycoproteoforms of haptoglobin and α1-acid glycoprotein using two different lectins Aleuria aurantia lectin (AAL) and Phaseolus vulgaris leucoagglutinin (PHA-L), respectively.
31183067	6	12	theme	Phaseolus	1093:1101	arg1	PHA-L					1129:1133	PHA-L	1129:1133	PHA-L	1129:1133	We investigate the interactions between the highly fucosylated and highly branched glycoproteoforms of haptoglobin and α1-acid glycoprotein using two different lectins Aleuria aurantia lectin (AAL) and Phaseolus vulgaris leucoagglutinin (PHA-L), respectively.
31183067	8	13	contain	has	1512:1514	arg1	it					1509:1510	it	1509:1510	it	1509:1510	Secondly, we analyze the global heterogeneity of highly branched glycoproteoforms of haptoglobin and α1-acid glycoprotein and reveal that while multi-fucosylation attenuates the lectin PHA-L binding to haptoglobin, it has no impact on AGP.
31183067	8	13	contain	has	1512:1514	arg2	impact					1519:1524	no impact	1516:1524	no impact on AGP	1516:1531	Secondly, we analyze the global heterogeneity of highly branched glycoproteoforms of haptoglobin and α1-acid glycoprotein and reveal that while multi-fucosylation attenuates the lectin PHA-L binding to haptoglobin, it has no impact on AGP.
31183067	10	14	theme	ovarian	1825:1831	arg1	cancers					1833:1839	ovarian cancers	1825:1839	ovarian cancers	1825:1839	Moreover, since aberrant glycosylation of Hp and AGP are potential markers for many diseases, including pancreatic, hepatic and ovarian cancers, understanding their interactions with lectins will help the development of carbohydrate-centric monitoring methods to understand their pathophysiological implications.
31183067	2	15	theme	many	277:280	arg1	pathways					303:310	many biological signaling pathways	277:310	many biological signaling pathways	277:310	Changes in lectin-reactive carbohydrate repertoires are related to many biological signaling pathways and recognized as hallmarks of several pathological processes.
31183067	1	16	theme	specific	159:166	arg1	epitopes					168:175	specific epitopes	159:175	specific epitopes present on target glycoproteins	159:207	Lectins are carbohydrate binding proteins that recognize specific epitopes present on target glycoproteins.
31183067	8	17	from	impact	1519:1524	arg1	AGP					1529:1531	AGP	1529:1531	AGP	1529:1531	Secondly, we analyze the global heterogeneity of highly branched glycoproteoforms of haptoglobin and α1-acid glycoprotein and reveal that while multi-fucosylation attenuates the lectin PHA-L binding to haptoglobin, it has no impact on AGP.
31183067	7	18	theme	N-glycan	1203:1210	arg1	branching					1212:1220	N-glycan branching	1203:1220	N-glycan branching	1203:1220	Firstly we show a co-occurrence of fucosylation and N-glycan branching on haptoglobin, particularly among highly fucosylated glycoproteoforms.
31183067	1	19	theme	present	177:183	arg1	epitopes					168:175	specific epitopes	159:175	specific epitopes present on target glycoproteins	159:207	Lectins are carbohydrate binding proteins that recognize specific epitopes present on target glycoproteins.
31183067	1	20	theme	target	188:193	arg1	glycoproteins					195:207	target glycoproteins	188:207	target glycoproteins	188:207	Lectins are carbohydrate binding proteins that recognize specific epitopes present on target glycoproteins.
31183067	1	21	gly	glycoproteins	195:207	arg1	glycoproteins					195:207	target glycoproteins	188:207	target glycoproteins	188:207	Lectins are carbohydrate binding proteins that recognize specific epitopes present on target glycoproteins.
31183067	5	22	theme	protein	876:882	arg1	level					884:888	the intact protein level	865:888	the intact protein level	865:888	Here, we apply a lectin-affinity purification procedure coupled with native mass spectrometry to characterize lectin-reactive glycoproteoforms at the intact protein level.
31183067	3	23	gly	glycoprotein	446:457	arg1	glycoprotein					446:457	glycoprotein structural and functional microheterogeneity	446:502	glycoprotein structural and functional microheterogeneity	446:502	Consequently, lectins are valuable probes, commonly used for examining glycoprotein structural and functional microheterogeneity.
31183067	1	24	theme	binding	127:133	arg1	Lectins					102:108	Lectins	102:108	Lectins	102:108	Lectins are carbohydrate binding proteins that recognize specific epitopes present on target glycoproteins.
31183067	1	24	theme	binding	127:133	arg1	proteins					135:142	carbohydrate binding proteins	114:142	carbohydrate binding proteins that recognize specific epitopes present on target glycoproteins	114:207	Lectins are carbohydrate binding proteins that recognize specific epitopes present on target glycoproteins.
31183067	3	25	theme	glycoprotein	446:457	arg1	microheterogeneity					485:502	glycoprotein structural and functional microheterogeneity	446:502	glycoprotein structural and functional microheterogeneity	446:502	Consequently, lectins are valuable probes, commonly used for examining glycoprotein structural and functional microheterogeneity.
31183067	6	26	theme	different	1041:1049	arg1	leucoagglutinin					1112:1126	Phaseolus vulgaris leucoagglutinin	1093:1126	Phaseolus vulgaris leucoagglutinin (PHA-L)	1093:1134	We investigate the interactions between the highly fucosylated and highly branched glycoproteoforms of haptoglobin and α1-acid glycoprotein using two different lectins Aleuria aurantia lectin (AAL) and Phaseolus vulgaris leucoagglutinin (PHA-L), respectively.
31183067	6	26	theme	different	1041:1049	arg1	lectins					1051:1057	two different lectins	1037:1057	two different lectins Aleuria aurantia lectin (AAL) and Phaseolus vulgaris leucoagglutinin (PHA-L)	1037:1134	We investigate the interactions between the highly fucosylated and highly branched glycoproteoforms of haptoglobin and α1-acid glycoprotein using two different lectins Aleuria aurantia lectin (AAL) and Phaseolus vulgaris leucoagglutinin (PHA-L), respectively.
31183067	6	26	theme	different	1041:1049	arg1	lectin					1076:1081	Aleuria aurantia lectin	1059:1081	Aleuria aurantia lectin (AAL)	1059:1087	We investigate the interactions between the highly fucosylated and highly branched glycoproteoforms of haptoglobin and α1-acid glycoprotein using two different lectins Aleuria aurantia lectin (AAL) and Phaseolus vulgaris leucoagglutinin (PHA-L), respectively.
31183067	4	27	theme	methods	669:675	arg1	complexity					639:648	the inherent complexity	626:648	the inherent complexity	626:648	However, the molecular interactions between a given lectin and its preferred glycoproteoforms are largely unknown due to the inherent complexity and limitations of methods used to investigate intact glycoproteins.
31183067	4	27	theme	methods	669:675	arg1	limitations					654:664	limitations	654:664	limitations	654:664	However, the molecular interactions between a given lectin and its preferred glycoproteoforms are largely unknown due to the inherent complexity and limitations of methods used to investigate intact glycoproteins.
31183067	2	28	theme	lectin-reactive	221:235	arg1	repertoires					250:260	lectin-reactive carbohydrate repertoires	221:260	lectin-reactive carbohydrate repertoires	221:260	Changes in lectin-reactive carbohydrate repertoires are related to many biological signaling pathways and recognized as hallmarks of several pathological processes.
31183067	10	29	dep	help	1893:1896	arg1	markers					1764:1770	potential markers	1754:1770	potential markers for many diseases, including pancreatic, hepatic and ovarian cancers	1754:1839	Moreover, since aberrant glycosylation of Hp and AGP are potential markers for many diseases, including pancreatic, hepatic and ovarian cancers, understanding their interactions with lectins will help the development of carbohydrate-centric monitoring methods to understand their pathophysiological implications.
31183067	10	29	dep	help	1893:1896	arg1	glycosylation					1722:1734	aberrant glycosylation	1713:1734	aberrant glycosylation of Hp and AGP	1713:1748	Moreover, since aberrant glycosylation of Hp and AGP are potential markers for many diseases, including pancreatic, hepatic and ovarian cancers, understanding their interactions with lectins will help the development of carbohydrate-centric monitoring methods to understand their pathophysiological implications.
31183067	0	30	theme	lectin	45:50	arg1	analysis					92:99	lectin affinity purification-mass spectrometry analysis	45:99	lectin affinity purification-mass spectrometry analysis	45:99	Probing N-glycoprotein microheterogeneity by lectin affinity purification-mass spectrometry analysis.
31183067	9	31	theme	lectin	1554:1559	arg1	approach					1593:1600	our lectin affinity purification native MS approach	1550:1600	our lectin affinity purification native MS approach	1550:1600	Taken together, our lectin affinity purification native MS approach elucidates lectin specificities between intact glycoproteins, not achievable by other methods.
31183067	0	32	gly	N-glycoprotein	8:21	arg1	N-glycoprotein					8:21	N-glycoprotein microheterogeneity	8:40	N-glycoprotein microheterogeneity	8:40	Probing N-glycoprotein microheterogeneity by lectin affinity purification-mass spectrometry analysis.
31183067	10	33	theme	AGP	1746:1748	arg1	markers					1764:1770	potential markers	1754:1770	potential markers for many diseases, including pancreatic, hepatic and ovarian cancers	1754:1839	Moreover, since aberrant glycosylation of Hp and AGP are potential markers for many diseases, including pancreatic, hepatic and ovarian cancers, understanding their interactions with lectins will help the development of carbohydrate-centric monitoring methods to understand their pathophysiological implications.
31183067	10	33	theme	AGP	1746:1748	arg1	glycosylation					1722:1734	aberrant glycosylation	1713:1734	aberrant glycosylation of Hp and AGP	1713:1748	Moreover, since aberrant glycosylation of Hp and AGP are potential markers for many diseases, including pancreatic, hepatic and ovarian cancers, understanding their interactions with lectins will help the development of carbohydrate-centric monitoring methods to understand their pathophysiological implications.
31183067	10	34	theme	methods	1949:1955	arg1	development					1902:1912	the development	1898:1912	the development of carbohydrate-centric monitoring methods	1898:1955	Moreover, since aberrant glycosylation of Hp and AGP are potential markers for many diseases, including pancreatic, hepatic and ovarian cancers, understanding their interactions with lectins will help the development of carbohydrate-centric monitoring methods to understand their pathophysiological implications.
31183067	0	35	theme	purification-mass	61:77	arg1	analysis					92:99	lectin affinity purification-mass spectrometry analysis	45:99	lectin affinity purification-mass spectrometry analysis	45:99	Probing N-glycoprotein microheterogeneity by lectin affinity purification-mass spectrometry analysis.
31183067	9	36	theme	purification	1570:1581	arg1	approach					1593:1600	our lectin affinity purification native MS approach	1550:1600	our lectin affinity purification native MS approach	1550:1600	Taken together, our lectin affinity purification native MS approach elucidates lectin specificities between intact glycoproteins, not achievable by other methods.
31183067	6	37	theme	α1-acid	1010:1016	arg1	glycoprotein					1018:1029	α1-acid glycoprotein	1010:1029	α1-acid glycoprotein	1010:1029	We investigate the interactions between the highly fucosylated and highly branched glycoproteoforms of haptoglobin and α1-acid glycoprotein using two different lectins Aleuria aurantia lectin (AAL) and Phaseolus vulgaris leucoagglutinin (PHA-L), respectively.
31183067	10	38	theme	Hp	1739:1740	arg1	markers					1764:1770	potential markers	1754:1770	potential markers for many diseases, including pancreatic, hepatic and ovarian cancers	1754:1839	Moreover, since aberrant glycosylation of Hp and AGP are potential markers for many diseases, including pancreatic, hepatic and ovarian cancers, understanding their interactions with lectins will help the development of carbohydrate-centric monitoring methods to understand their pathophysiological implications.
31183067	10	38	theme	Hp	1739:1740	arg1	glycosylation					1722:1734	aberrant glycosylation	1713:1734	aberrant glycosylation of Hp and AGP	1713:1748	Moreover, since aberrant glycosylation of Hp and AGP are potential markers for many diseases, including pancreatic, hepatic and ovarian cancers, understanding their interactions with lectins will help the development of carbohydrate-centric monitoring methods to understand their pathophysiological implications.
31183067	10	39	theme	carbohydrate-centric	1917:1936	arg1	methods					1949:1955	carbohydrate-centric monitoring methods	1917:1955	carbohydrate-centric monitoring methods	1917:1955	Moreover, since aberrant glycosylation of Hp and AGP are potential markers for many diseases, including pancreatic, hepatic and ovarian cancers, understanding their interactions with lectins will help the development of carbohydrate-centric monitoring methods to understand their pathophysiological implications.
31183067	9	40	theme	MS	1590:1591	arg1	approach					1593:1600	our lectin affinity purification native MS approach	1550:1600	our lectin affinity purification native MS approach	1550:1600	Taken together, our lectin affinity purification native MS approach elucidates lectin specificities between intact glycoproteins, not achievable by other methods.
31183067	6	41	theme	haptoglobin	994:1004	arg1	glycoproteoforms					974:989	the highly fucosylated and highly branched glycoproteoforms	931:989	the highly fucosylated and highly branched glycoproteoforms of haptoglobin and α1-acid glycoprotein	931:1029	We investigate the interactions between the highly fucosylated and highly branched glycoproteoforms of haptoglobin and α1-acid glycoprotein using two different lectins Aleuria aurantia lectin (AAL) and Phaseolus vulgaris leucoagglutinin (PHA-L), respectively.
31183067	6	42	dep	lectins	1051:1057	arg1	leucoagglutinin					1112:1126	Phaseolus vulgaris leucoagglutinin	1093:1126	Phaseolus vulgaris leucoagglutinin (PHA-L)	1093:1134	We investigate the interactions between the highly fucosylated and highly branched glycoproteoforms of haptoglobin and α1-acid glycoprotein using two different lectins Aleuria aurantia lectin (AAL) and Phaseolus vulgaris leucoagglutinin (PHA-L), respectively.
31183067	6	42	dep	lectins	1051:1057	arg1	AAL					1084:1086	AAL	1084:1086	AAL	1084:1086	We investigate the interactions between the highly fucosylated and highly branched glycoproteoforms of haptoglobin and α1-acid glycoprotein using two different lectins Aleuria aurantia lectin (AAL) and Phaseolus vulgaris leucoagglutinin (PHA-L), respectively.
31183067	6	42	dep	lectins	1051:1057	arg1	lectins					1051:1057	two different lectins	1037:1057	two different lectins Aleuria aurantia lectin (AAL) and Phaseolus vulgaris leucoagglutinin (PHA-L)	1037:1134	We investigate the interactions between the highly fucosylated and highly branched glycoproteoforms of haptoglobin and α1-acid glycoprotein using two different lectins Aleuria aurantia lectin (AAL) and Phaseolus vulgaris leucoagglutinin (PHA-L), respectively.
31183067	6	42	dep	lectins	1051:1057	arg1	lectin					1076:1081	Aleuria aurantia lectin	1059:1081	Aleuria aurantia lectin (AAL)	1059:1087	We investigate the interactions between the highly fucosylated and highly branched glycoproteoforms of haptoglobin and α1-acid glycoprotein using two different lectins Aleuria aurantia lectin (AAL) and Phaseolus vulgaris leucoagglutinin (PHA-L), respectively.
31183067	6	42	dep	lectins	1051:1057	arg1	PHA-L					1129:1133	PHA-L	1129:1133	PHA-L	1129:1133	We investigate the interactions between the highly fucosylated and highly branched glycoproteoforms of haptoglobin and α1-acid glycoprotein using two different lectins Aleuria aurantia lectin (AAL) and Phaseolus vulgaris leucoagglutinin (PHA-L), respectively.
31183067	9	43	gly	glycoproteins	1649:1661	arg1	glycoproteins					1649:1661	intact glycoproteins	1642:1661	intact glycoproteins	1642:1661	Taken together, our lectin affinity purification native MS approach elucidates lectin specificities between intact glycoproteins, not achievable by other methods.
31183067	10	44	theme	pathophysiological	1977:1994	arg1	implications					1996:2007	their pathophysiological implications	1971:2007	their pathophysiological implications	1971:2007	Moreover, since aberrant glycosylation of Hp and AGP are potential markers for many diseases, including pancreatic, hepatic and ovarian cancers, understanding their interactions with lectins will help the development of carbohydrate-centric monitoring methods to understand their pathophysiological implications.
31183067	2	45	from	Changes	210:216	arg1	repertoires					250:260	lectin-reactive carbohydrate repertoires	221:260	lectin-reactive carbohydrate repertoires	221:260	Changes in lectin-reactive carbohydrate repertoires are related to many biological signaling pathways and recognized as hallmarks of several pathological processes.
31183067	10	46	with	interactions	1862:1873	arg1	lectins					1880:1886	lectins	1880:1886	lectins	1880:1886	Moreover, since aberrant glycosylation of Hp and AGP are potential markers for many diseases, including pancreatic, hepatic and ovarian cancers, understanding their interactions with lectins will help the development of carbohydrate-centric monitoring methods to understand their pathophysiological implications.
31183067	10	47	theme	potential	1754:1762	arg1	markers					1764:1770	potential markers	1754:1770	potential markers for many diseases, including pancreatic, hepatic and ovarian cancers	1754:1839	Moreover, since aberrant glycosylation of Hp and AGP are potential markers for many diseases, including pancreatic, hepatic and ovarian cancers, understanding their interactions with lectins will help the development of carbohydrate-centric monitoring methods to understand their pathophysiological implications.
31183067	10	47	theme	potential	1754:1762	arg1	glycosylation					1722:1734	aberrant glycosylation	1713:1734	aberrant glycosylation of Hp and AGP	1713:1748	Moreover, since aberrant glycosylation of Hp and AGP are potential markers for many diseases, including pancreatic, hepatic and ovarian cancers, understanding their interactions with lectins will help the development of carbohydrate-centric monitoring methods to understand their pathophysiological implications.
31183067	6	48	theme	branched	965:972	arg1	glycoproteoforms					974:989	the highly fucosylated and highly branched glycoproteoforms	931:989	the highly fucosylated and highly branched glycoproteoforms of haptoglobin and α1-acid glycoprotein	931:1029	We investigate the interactions between the highly fucosylated and highly branched glycoproteoforms of haptoglobin and α1-acid glycoprotein using two different lectins Aleuria aurantia lectin (AAL) and Phaseolus vulgaris leucoagglutinin (PHA-L), respectively.
31183067	2	49	theme	processes	364:372	arg1	Changes					210:216	Changes	210:216	Changes in lectin-reactive carbohydrate repertoires	210:260	Changes in lectin-reactive carbohydrate repertoires are related to many biological signaling pathways and recognized as hallmarks of several pathological processes.
31183067	2	49	theme	processes	364:372	arg1	hallmarks					330:338	hallmarks	330:338	hallmarks of several pathological processes	330:372	Changes in lectin-reactive carbohydrate repertoires are related to many biological signaling pathways and recognized as hallmarks of several pathological processes.
31183067	9	50	theme	intact	1642:1647	arg1	glycoproteins					1649:1661	intact glycoproteins	1642:1661	intact glycoproteins	1642:1661	Taken together, our lectin affinity purification native MS approach elucidates lectin specificities between intact glycoproteins, not achievable by other methods.
31183067	4	51	theme	intact	697:702	arg1	glycoproteins					704:716	intact glycoproteins	697:716	intact glycoproteins	697:716	However, the molecular interactions between a given lectin and its preferred glycoproteoforms are largely unknown due to the inherent complexity and limitations of methods used to investigate intact glycoproteins.
31183067	8	52	gly	glycoprotein	1403:1414	arg1	glycoprotein					1403:1414	α1-acid glycoprotein	1395:1414	α1-acid glycoprotein	1395:1414	Secondly, we analyze the global heterogeneity of highly branched glycoproteoforms of haptoglobin and α1-acid glycoprotein and reveal that while multi-fucosylation attenuates the lectin PHA-L binding to haptoglobin, it has no impact on AGP.
31183067	2	53	theme	several	343:349	arg1	processes					364:372	several pathological processes	343:372	several pathological processes	343:372	Changes in lectin-reactive carbohydrate repertoires are related to many biological signaling pathways and recognized as hallmarks of several pathological processes.
31183067	7	54	gly	fucosylated	1264:1274	arg1	glycoproteoforms					1276:1291	highly fucosylated glycoproteoforms	1257:1291	highly fucosylated glycoproteoforms	1257:1291	Firstly we show a co-occurrence of fucosylation and N-glycan branching on haptoglobin, particularly among highly fucosylated glycoproteoforms.
31183067	8	55	theme	glycoproteoforms	1359:1374	arg1	heterogeneity					1326:1338	the global heterogeneity	1315:1338	the global heterogeneity of highly branched glycoproteoforms of haptoglobin and α1-acid glycoprotein	1315:1414	Secondly, we analyze the global heterogeneity of highly branched glycoproteoforms of haptoglobin and α1-acid glycoprotein and reveal that while multi-fucosylation attenuates the lectin PHA-L binding to haptoglobin, it has no impact on AGP.
31183067	6	56	theme	Aleuria	1059:1065	arg1	AAL					1084:1086	AAL	1084:1086	AAL	1084:1086	We investigate the interactions between the highly fucosylated and highly branched glycoproteoforms of haptoglobin and α1-acid glycoprotein using two different lectins Aleuria aurantia lectin (AAL) and Phaseolus vulgaris leucoagglutinin (PHA-L), respectively.
31183067	6	56	theme	Aleuria	1059:1065	arg1	lectins					1051:1057	two different lectins	1037:1057	two different lectins Aleuria aurantia lectin (AAL) and Phaseolus vulgaris leucoagglutinin (PHA-L)	1037:1134	We investigate the interactions between the highly fucosylated and highly branched glycoproteoforms of haptoglobin and α1-acid glycoprotein using two different lectins Aleuria aurantia lectin (AAL) and Phaseolus vulgaris leucoagglutinin (PHA-L), respectively.
31183067	6	56	theme	Aleuria	1059:1065	arg1	lectin					1076:1081	Aleuria aurantia lectin	1059:1081	Aleuria aurantia lectin (AAL)	1059:1087	We investigate the interactions between the highly fucosylated and highly branched glycoproteoforms of haptoglobin and α1-acid glycoprotein using two different lectins Aleuria aurantia lectin (AAL) and Phaseolus vulgaris leucoagglutinin (PHA-L), respectively.
31183067	5	57	theme	lectin-affinity	736:750	arg1	procedure					765:773	a lectin-affinity purification procedure	734:773	a lectin-affinity purification procedure coupled with native mass spectrometry to characterize lectin-reactive glycoproteoforms at the intact protein level	734:888	Here, we apply a lectin-affinity purification procedure coupled with native mass spectrometry to characterize lectin-reactive glycoproteoforms at the intact protein level.
31183067	9	58	theme	achievable	1668:1677	arg1	specificities					1620:1632	lectin specificities	1613:1632	lectin specificities	1613:1632	Taken together, our lectin affinity purification native MS approach elucidates lectin specificities between intact glycoproteins, not achievable by other methods.
31183067	8	59	theme	haptoglobin	1379:1389	arg1	glycoproteoforms					1359:1374	highly branched glycoproteoforms	1343:1374	highly branched glycoproteoforms of haptoglobin and α1-acid glycoprotein	1343:1414	Secondly, we analyze the global heterogeneity of highly branched glycoproteoforms of haptoglobin and α1-acid glycoprotein and reveal that while multi-fucosylation attenuates the lectin PHA-L binding to haptoglobin, it has no impact on AGP.
31183067	5	60	theme	purification	752:763	arg1	procedure					765:773	a lectin-affinity purification procedure	734:773	a lectin-affinity purification procedure coupled with native mass spectrometry to characterize lectin-reactive glycoproteoforms at the intact protein level	734:888	Here, we apply a lectin-affinity purification procedure coupled with native mass spectrometry to characterize lectin-reactive glycoproteoforms at the intact protein level.
31183067	5	61	theme	lectin-reactive	829:843	arg1	glycoproteoforms					845:860	lectin-reactive glycoproteoforms	829:860	lectin-reactive glycoproteoforms	829:860	Here, we apply a lectin-affinity purification procedure coupled with native mass spectrometry to characterize lectin-reactive glycoproteoforms at the intact protein level.
31183067	9	62	theme	other	1682:1686	arg1	methods					1688:1694	other methods	1682:1694	other methods	1682:1694	Taken together, our lectin affinity purification native MS approach elucidates lectin specificities between intact glycoproteins, not achievable by other methods.
31183067	8	63	theme	α1-acid	1395:1401	arg1	glycoprotein					1403:1414	α1-acid glycoprotein	1395:1414	α1-acid glycoprotein	1395:1414	Secondly, we analyze the global heterogeneity of highly branched glycoproteoforms of haptoglobin and α1-acid glycoprotein and reveal that while multi-fucosylation attenuates the lectin PHA-L binding to haptoglobin, it has no impact on AGP.
31183067	4	64	theme	given	551:555	arg1	lectin					557:562	a given lectin	549:562	a given lectin	549:562	However, the molecular interactions between a given lectin and its preferred glycoproteoforms are largely unknown due to the inherent complexity and limitations of methods used to investigate intact glycoproteins.
31183067	8	65	theme	PHA-L	1479:1483	arg1	binding					1485:1491	the lectin PHA-L binding	1468:1491	the lectin PHA-L binding to haptoglobin	1468:1506	Secondly, we analyze the global heterogeneity of highly branched glycoproteoforms of haptoglobin and α1-acid glycoprotein and reveal that while multi-fucosylation attenuates the lectin PHA-L binding to haptoglobin, it has no impact on AGP.
31183067	5	66	theme	mass	795:798	arg1	spectrometry					800:811	native mass spectrometry	788:811	native mass spectrometry to characterize lectin-reactive glycoproteoforms at the intact protein level	788:888	Here, we apply a lectin-affinity purification procedure coupled with native mass spectrometry to characterize lectin-reactive glycoproteoforms at the intact protein level.
31183067	2	67	theme	biological	282:291	arg1	pathways					303:310	many biological signaling pathways	277:310	many biological signaling pathways	277:310	Changes in lectin-reactive carbohydrate repertoires are related to many biological signaling pathways and recognized as hallmarks of several pathological processes.
31183067	7	68	theme	fucosylation	1186:1197	arg1	branching					1212:1220	N-glycan branching	1203:1220	N-glycan branching	1203:1220	Firstly we show a co-occurrence of fucosylation and N-glycan branching on haptoglobin, particularly among highly fucosylated glycoproteoforms.
31183067	7	68	theme	fucosylation	1186:1197	arg1	co-occurrence					1169:1181	a co-occurrence	1167:1181	a co-occurrence of fucosylation	1167:1197	Firstly we show a co-occurrence of fucosylation and N-glycan branching on haptoglobin, particularly among highly fucosylated glycoproteoforms.
31183067	6	69	theme	vulgaris	1103:1110	arg1	leucoagglutinin					1112:1126	Phaseolus vulgaris leucoagglutinin	1093:1126	Phaseolus vulgaris leucoagglutinin (PHA-L)	1093:1134	We investigate the interactions between the highly fucosylated and highly branched glycoproteoforms of haptoglobin and α1-acid glycoprotein using two different lectins Aleuria aurantia lectin (AAL) and Phaseolus vulgaris leucoagglutinin (PHA-L), respectively.
31183067	6	69	theme	vulgaris	1103:1110	arg1	lectins					1051:1057	two different lectins	1037:1057	two different lectins Aleuria aurantia lectin (AAL) and Phaseolus vulgaris leucoagglutinin (PHA-L)	1037:1134	We investigate the interactions between the highly fucosylated and highly branched glycoproteoforms of haptoglobin and α1-acid glycoprotein using two different lectins Aleuria aurantia lectin (AAL) and Phaseolus vulgaris leucoagglutinin (PHA-L), respectively.
31183067	6	69	theme	vulgaris	1103:1110	arg1	PHA-L					1129:1133	PHA-L	1129:1133	PHA-L	1129:1133	We investigate the interactions between the highly fucosylated and highly branched glycoproteoforms of haptoglobin and α1-acid glycoprotein using two different lectins Aleuria aurantia lectin (AAL) and Phaseolus vulgaris leucoagglutinin (PHA-L), respectively.
31183067	4	70	theme	molecular	518:526	arg1	interactions					528:539	the molecular interactions	514:539	the molecular interactions between a given lectin and its preferred glycoproteoforms	514:597	However, the molecular interactions between a given lectin and its preferred glycoproteoforms are largely unknown due to the inherent complexity and limitations of methods used to investigate intact glycoproteins.
31183067	4	70	theme	molecular	518:526	arg1	unknown					611:617	unknown	611:617	unknown	611:617	However, the molecular interactions between a given lectin and its preferred glycoproteoforms are largely unknown due to the inherent complexity and limitations of methods used to investigate intact glycoproteins.
31183067	3	71	theme	valuable	401:408	arg1	probes					410:415	valuable probes	401:415	valuable probes	401:415	Consequently, lectins are valuable probes, commonly used for examining glycoprotein structural and functional microheterogeneity.
31183067	3	71	theme	valuable	401:408	arg1	lectins					389:395	lectins	389:395	lectins	389:395	Consequently, lectins are valuable probes, commonly used for examining glycoprotein structural and functional microheterogeneity.
31183067	5	72	theme	intact	869:874	arg1	level					884:888	the intact protein level	865:888	the intact protein level	865:888	Here, we apply a lectin-affinity purification procedure coupled with native mass spectrometry to characterize lectin-reactive glycoproteoforms at the intact protein level.
31183067	4	73	theme	preferred	572:580	arg1	glycoproteoforms					582:597	its preferred glycoproteoforms	568:597	its preferred glycoproteoforms	568:597	However, the molecular interactions between a given lectin and its preferred glycoproteoforms are largely unknown due to the inherent complexity and limitations of methods used to investigate intact glycoproteins.
31183067	0	74	theme	N-glycoprotein	8:21	arg1	microheterogeneity					23:40	N-glycoprotein microheterogeneity	8:40	N-glycoprotein microheterogeneity	8:40	Probing N-glycoprotein microheterogeneity by lectin affinity purification-mass spectrometry analysis.
31183067	1	75	theme	carbohydrate	114:125	arg1	Lectins					102:108	Lectins	102:108	Lectins	102:108	Lectins are carbohydrate binding proteins that recognize specific epitopes present on target glycoproteins.
31183067	1	75	theme	carbohydrate	114:125	arg1	proteins					135:142	carbohydrate binding proteins	114:142	carbohydrate binding proteins that recognize specific epitopes present on target glycoproteins	114:207	Lectins are carbohydrate binding proteins that recognize specific epitopes present on target glycoproteins.
31183067	3	76	theme	functional	474:483	arg1	microheterogeneity					485:502	glycoprotein structural and functional microheterogeneity	446:502	glycoprotein structural and functional microheterogeneity	446:502	Consequently, lectins are valuable probes, commonly used for examining glycoprotein structural and functional microheterogeneity.
31183067	4	77	gly	glycoproteins	704:716	arg1	glycoproteins					704:716	intact glycoproteins	697:716	intact glycoproteins	697:716	However, the molecular interactions between a given lectin and its preferred glycoproteoforms are largely unknown due to the inherent complexity and limitations of methods used to investigate intact glycoproteins.
31183067	3	78	theme	structural	459:468	arg1	microheterogeneity					485:502	glycoprotein structural and functional microheterogeneity	446:502	glycoprotein structural and functional microheterogeneity	446:502	Consequently, lectins are valuable probes, commonly used for examining glycoprotein structural and functional microheterogeneity.
31183067	10	79	theme	aberrant	1713:1720	arg1	markers					1764:1770	potential markers	1754:1770	potential markers for many diseases, including pancreatic, hepatic and ovarian cancers	1754:1839	Moreover, since aberrant glycosylation of Hp and AGP are potential markers for many diseases, including pancreatic, hepatic and ovarian cancers, understanding their interactions with lectins will help the development of carbohydrate-centric monitoring methods to understand their pathophysiological implications.
31183067	10	79	theme	aberrant	1713:1720	arg1	glycosylation					1722:1734	aberrant glycosylation	1713:1734	aberrant glycosylation of Hp and AGP	1713:1748	Moreover, since aberrant glycosylation of Hp and AGP are potential markers for many diseases, including pancreatic, hepatic and ovarian cancers, understanding their interactions with lectins will help the development of carbohydrate-centric monitoring methods to understand their pathophysiological implications.
31183067	2	80	theme	carbohydrate	237:248	arg1	repertoires					250:260	lectin-reactive carbohydrate repertoires	221:260	lectin-reactive carbohydrate repertoires	221:260	Changes in lectin-reactive carbohydrate repertoires are related to many biological signaling pathways and recognized as hallmarks of several pathological processes.
31183067	6	81	gly	glycoprotein	1018:1029	arg1	glycoprotein					1018:1029	α1-acid glycoprotein	1010:1029	α1-acid glycoprotein	1010:1029	We investigate the interactions between the highly fucosylated and highly branched glycoproteoforms of haptoglobin and α1-acid glycoprotein using two different lectins Aleuria aurantia lectin (AAL) and Phaseolus vulgaris leucoagglutinin (PHA-L), respectively.
31183067	0	82	theme	affinity	52:59	arg1	analysis					92:99	lectin affinity purification-mass spectrometry analysis	45:99	lectin affinity purification-mass spectrometry analysis	45:99	Probing N-glycoprotein microheterogeneity by lectin affinity purification-mass spectrometry analysis.
31183067	4	83	theme	inherent	630:637	arg1	complexity					639:648	the inherent complexity	626:648	the inherent complexity	626:648	However, the molecular interactions between a given lectin and its preferred glycoproteoforms are largely unknown due to the inherent complexity and limitations of methods used to investigate intact glycoproteins.
31183067	0	84	theme	spectrometry	79:90	arg1	analysis					92:99	lectin affinity purification-mass spectrometry analysis	45:99	lectin affinity purification-mass spectrometry analysis	45:99	Probing N-glycoprotein microheterogeneity by lectin affinity purification-mass spectrometry analysis.
31183067	9	85	theme	affinity	1561:1568	arg1	approach					1593:1600	our lectin affinity purification native MS approach	1550:1600	our lectin affinity purification native MS approach	1550:1600	Taken together, our lectin affinity purification native MS approach elucidates lectin specificities between intact glycoproteins, not achievable by other methods.
31183067	6	86	theme	glycoprotein	1018:1029	arg1	glycoproteoforms					974:989	the highly fucosylated and highly branched glycoproteoforms	931:989	the highly fucosylated and highly branched glycoproteoforms of haptoglobin and α1-acid glycoprotein	931:1029	We investigate the interactions between the highly fucosylated and highly branched glycoproteoforms of haptoglobin and α1-acid glycoprotein using two different lectins Aleuria aurantia lectin (AAL) and Phaseolus vulgaris leucoagglutinin (PHA-L), respectively.
31183067	8	87	gly	heterogeneity	1326:1338	arg1	glycoproteoforms					1359:1374	highly branched glycoproteoforms	1343:1374	highly branched glycoproteoforms of haptoglobin and α1-acid glycoprotein	1343:1414	Secondly, we analyze the global heterogeneity of highly branched glycoproteoforms of haptoglobin and α1-acid glycoprotein and reveal that while multi-fucosylation attenuates the lectin PHA-L binding to haptoglobin, it has no impact on AGP.
31183067	10	88	theme	monitoring	1938:1947	arg1	methods					1949:1955	carbohydrate-centric monitoring methods	1917:1955	carbohydrate-centric monitoring methods	1917:1955	Moreover, since aberrant glycosylation of Hp and AGP are potential markers for many diseases, including pancreatic, hepatic and ovarian cancers, understanding their interactions with lectins will help the development of carbohydrate-centric monitoring methods to understand their pathophysiological implications.
31183067	9	89	theme	native	1583:1588	arg1	approach					1593:1600	our lectin affinity purification native MS approach	1550:1600	our lectin affinity purification native MS approach	1550:1600	Taken together, our lectin affinity purification native MS approach elucidates lectin specificities between intact glycoproteins, not achievable by other methods.
31183067	6	90	dep	Aleuria	1059:1065	arg1	aurantia					1067:1074	aurantia	1067:1074	aurantia	1067:1074	We investigate the interactions between the highly fucosylated and highly branched glycoproteoforms of haptoglobin and α1-acid glycoprotein using two different lectins Aleuria aurantia lectin (AAL) and Phaseolus vulgaris leucoagglutinin (PHA-L), respectively.
31183067	8	91	theme	global	1319:1324	arg1	heterogeneity					1326:1338	the global heterogeneity	1315:1338	the global heterogeneity of highly branched glycoproteoforms of haptoglobin and α1-acid glycoprotein	1315:1414	Secondly, we analyze the global heterogeneity of highly branched glycoproteoforms of haptoglobin and α1-acid glycoprotein and reveal that while multi-fucosylation attenuates the lectin PHA-L binding to haptoglobin, it has no impact on AGP.
31183067	9	92	theme	lectin	1613:1618	arg1	specificities					1620:1632	lectin specificities	1613:1632	lectin specificities	1613:1632	Taken together, our lectin affinity purification native MS approach elucidates lectin specificities between intact glycoproteins, not achievable by other methods.
29616046	12	0	theme	adjuvanted	2329:2338	arg1	candidate					2445:2453	an effective, non-toxic, second generation subunit-vaccine candidate	2386:2453	an effective, non-toxic, second generation subunit-vaccine candidate against anthrax	2386:2469	Altogether, the results suggest that CpG or Poly I:C adjuvanted, PA-loaded TMC nanoparticles could be used as an effective, non-toxic, second generation subunit-vaccine candidate against anthrax.
29616046	12	0	theme	adjuvanted	2329:2338	arg1	nanoparticles					2355:2367	Poly I:C adjuvanted, PA-loaded TMC nanoparticles	2320:2367	nanoparticles	2355:2367	Altogether, the results suggest that CpG or Poly I:C adjuvanted, PA-loaded TMC nanoparticles could be used as an effective, non-toxic, second generation subunit-vaccine candidate against anthrax.
29616046	12	0	theme	adjuvanted	2329:2338	arg1	CpG					2313:2315	CpG	2313:2315	CpG	2313:2315	Altogether, the results suggest that CpG or Poly I:C adjuvanted, PA-loaded TMC nanoparticles could be used as an effective, non-toxic, second generation subunit-vaccine candidate against anthrax.
29616046	8	1	theme	Th	1663:1664	arg1	development					1672:1682	T-helper (Th) cell development	1653:1682	T-helper (Th) cell development	1653:1682	The effect of formulations on T-helper (Th) cell development was assessed by quantifying the Th1-dependant (TNF-α, IFN-γ, and IL-2), Th2-dependant (IL-4, IL-6, and IL-10), and Th17-type (IL-17A) cytokines.
29616046	6	2	theme	synergistic	1323:1333	arg1	behavior					1335:1342	the synergistic behavior	1319:1342	the synergistic behavior of CpG and Poly I:C with TMC nanoparticles	1319:1385	This clearly demonstrates the synergistic behavior of CpG and Poly I:C with TMC nanoparticles.
29616046	3	3	contain	contains	578:585	arg2	components					615:624	well-defined and controlled components	587:624	well-defined and controlled components	587:624	Hence, an anthrax vaccine that contains well-defined and controlled components would be highly desirable.
29616046	3	3	contain	contains	578:585	arg1	desirable					642:650	desirable	642:650	desirable	642:650	Hence, an anthrax vaccine that contains well-defined and controlled components would be highly desirable.
29616046	3	3	contain	contains	578:585	arg1	vaccine					565:571	an anthrax vaccine	554:571	an anthrax vaccine that contains well-defined and controlled components	554:624	Hence, an anthrax vaccine that contains well-defined and controlled components would be highly desirable.
29616046	1	4	dep	are	157:159	arg1	precipitated					243:254	precipitated	243:254	precipitated	243:254	Anthrax is an era old deadly disease against which there are only two currently available licensed vaccines named anthrax vaccine adsorbed and precipitated (AVP).
29616046	1	4	dep	are	157:159	arg1	adsorbed					230:237	adsorbed	230:237	adsorbed	230:237	Anthrax is an era old deadly disease against which there are only two currently available licensed vaccines named anthrax vaccine adsorbed and precipitated (AVP).
29616046	8	5	theme	cell	1667:1670	arg1	development					1672:1682	T-helper (Th) cell development	1653:1682	T-helper (Th) cell development	1653:1682	The effect of formulations on T-helper (Th) cell development was assessed by quantifying the Th1-dependant (TNF-α, IFN-γ, and IL-2), Th2-dependant (IL-4, IL-6, and IL-10), and Th17-type (IL-17A) cytokines.
29616046	8	6	dep	Th2-dependant	1756:1768	arg1	IL-10					1787:1791	IL-10	1787:1791	IL-10	1787:1791	The effect of formulations on T-helper (Th) cell development was assessed by quantifying the Th1-dependant (TNF-α, IFN-γ, and IL-2), Th2-dependant (IL-4, IL-6, and IL-10), and Th17-type (IL-17A) cytokines.
29616046	8	6	dep	Th2-dependant	1756:1768	arg1	IL-4					1771:1774	IL-4	1771:1774	IL-4	1771:1774	The effect of formulations on T-helper (Th) cell development was assessed by quantifying the Th1-dependant (TNF-α, IFN-γ, and IL-2), Th2-dependant (IL-4, IL-6, and IL-10), and Th17-type (IL-17A) cytokines.
29616046	8	6	dep	Th2-dependant	1756:1768	arg1	IL-6					1777:1780	IL-6	1777:1780	IL-6	1777:1780	The effect of formulations on T-helper (Th) cell development was assessed by quantifying the Th1-dependant (TNF-α, IFN-γ, and IL-2), Th2-dependant (IL-4, IL-6, and IL-10), and Th17-type (IL-17A) cytokines.
29616046	9	7	theme	TMC-PA	1869:1874	arg1	I					1860:1860	CpG and Poly I	1847:1860	I	1860:1860	Adjuvanation with CpG and Poly I:C, the TMC-PA nanoparticles triggered a Th1 skewed immune response, as suggested by an increase in the levels of total IgG2a along with IFN-γ cytokine production.
29616046	9	7	theme	TMC-PA	1869:1874	arg1	nanoparticles					1876:1888	the TMC-PA nanoparticles	1865:1888	the TMC-PA nanoparticles	1865:1888	Adjuvanation with CpG and Poly I:C, the TMC-PA nanoparticles triggered a Th1 skewed immune response, as suggested by an increase in the levels of total IgG2a along with IFN-γ cytokine production.
29616046	4	8	dep	intramuscular	975:987	arg1	IM					990:991	IM	990:991	IM	990:991	In this context, we have evaluated the potential of various vaccine formulations comprising of protective antigen (PA) encapsulated trimethyl-chitosan nanoparticles (TMC-PA) in conjunction with either CpG-C ODN 2395 (CpG) or Poly I:C. Each formulation was administered via three different routes, viz., subcutaneous (SC), intramuscular (IM), and intraperitoneal in female BALB/c mice.
29616046	4	9	theme	CpG-C	854:858	arg1	ODN					860:862	CpG-C ODN 2395	854:867	CpG-C ODN 2395 (CpG)	854:873	In this context, we have evaluated the potential of various vaccine formulations comprising of protective antigen (PA) encapsulated trimethyl-chitosan nanoparticles (TMC-PA) in conjunction with either CpG-C ODN 2395 (CpG) or Poly I:C. Each formulation was administered via three different routes, viz., subcutaneous (SC), intramuscular (IM), and intraperitoneal in female BALB/c mice.
29616046	4	9	theme	CpG-C	854:858	arg1	CpG					870:872	CpG	870:872	CpG	870:872	In this context, we have evaluated the potential of various vaccine formulations comprising of protective antigen (PA) encapsulated trimethyl-chitosan nanoparticles (TMC-PA) in conjunction with either CpG-C ODN 2395 (CpG) or Poly I:C. Each formulation was administered via three different routes, viz., subcutaneous (SC), intramuscular (IM), and intraperitoneal in female BALB/c mice.
29616046	7	10	theme	PA	1600:1601	arg1	2-8 × 104					1611:1619	2-8 × 104	1611:1619	2-8 × 104	1611:1619	The adjuvant potential of TMC nanoparticles could be observed in all the three routes as the TMC-PA nanoparticles by themselves induced IgG titers (1-1.5 × 105) significantly higher than both CpG PA and Poly I:C PA groups (2-8 × 104).
29616046	7	10	theme	PA	1600:1601	arg1	groups					1603:1608	both CpG PA and Poly I:C PA groups	1575:1608	both CpG PA and Poly I:C PA groups (2-8 × 104)	1575:1620	The adjuvant potential of TMC nanoparticles could be observed in all the three routes as the TMC-PA nanoparticles by themselves induced IgG titers (1-1.5 × 105) significantly higher than both CpG PA and Poly I:C PA groups (2-8 × 104).
29616046	12	11	theme	C	2327:2327	arg1	candidate					2445:2453	an effective, non-toxic, second generation subunit-vaccine candidate	2386:2453	an effective, non-toxic, second generation subunit-vaccine candidate against anthrax	2386:2469	Altogether, the results suggest that CpG or Poly I:C adjuvanted, PA-loaded TMC nanoparticles could be used as an effective, non-toxic, second generation subunit-vaccine candidate against anthrax.
29616046	12	11	theme	C	2327:2327	arg1	nanoparticles					2355:2367	Poly I:C adjuvanted, PA-loaded TMC nanoparticles	2320:2367	nanoparticles	2355:2367	Altogether, the results suggest that CpG or Poly I:C adjuvanted, PA-loaded TMC nanoparticles could be used as an effective, non-toxic, second generation subunit-vaccine candidate against anthrax.
29616046	12	11	theme	C	2327:2327	arg1	CpG					2313:2315	CpG	2313:2315	CpG	2313:2315	Altogether, the results suggest that CpG or Poly I:C adjuvanted, PA-loaded TMC nanoparticles could be used as an effective, non-toxic, second generation subunit-vaccine candidate against anthrax.
29616046	6	12	with	behavior	1335:1342	arg1	nanoparticles					1373:1385	TMC nanoparticles	1369:1385	TMC nanoparticles	1369:1385	This clearly demonstrates the synergistic behavior of CpG and Poly I:C with TMC nanoparticles.
29616046	5	13	theme	PA	1276:1277	arg1	counterparts					1279:1290	C PA counterparts	1274:1290	Poly I:C PA counterparts	1267:1290	Irrespective of the route of immunization, CpG or Poly I:C adjuvanted TMC-PA nanoparticles induced a significantly higher humoral response (total serum IgG and its isotypes viz., IgG1, IgG2a, and IgG2b), compared to their CpG or Poly I:C PA counterparts.
29616046	6	14	theme	TMC	1369:1371	arg1	nanoparticles					1373:1385	TMC nanoparticles	1369:1385	TMC nanoparticles	1369:1385	This clearly demonstrates the synergistic behavior of CpG and Poly I:C with TMC nanoparticles.
29616046	1	15	theme	licensed	190:197	arg1	vaccines					199:206	two currently available licensed vaccines	166:206	two currently available licensed vaccines named anthrax vaccine	166:228	Anthrax is an era old deadly disease against which there are only two currently available licensed vaccines named anthrax vaccine adsorbed and precipitated (AVP).
29616046	2	16	theme	objectionable	532:544	arg1	use					528:530	their widespread use objectionable	511:544	their widespread use objectionable	511:544	Though they can provide a protective immunity, their multiple side-effects owing to their ill-defined composition and presence of toxic proteins (LF and EF) of Bacillus anthracis, the causative organism of anthrax, in the vaccine formulation makes their widespread use objectionable.
29616046	2	17	from	composition	365:375	arg1	formulation					493:503	the vaccine formulation	481:503	the vaccine formulation	481:503	Though they can provide a protective immunity, their multiple side-effects owing to their ill-defined composition and presence of toxic proteins (LF and EF) of Bacillus anthracis, the causative organism of anthrax, in the vaccine formulation makes their widespread use objectionable.
29616046	9	18	theme	Th1	1902:1904	arg1	response					1920:1927	a Th1 skewed immune response	1900:1927	a Th1 skewed immune response	1900:1927	Adjuvanation with CpG and Poly I:C, the TMC-PA nanoparticles triggered a Th1 skewed immune response, as suggested by an increase in the levels of total IgG2a along with IFN-γ cytokine production.
29616046	2	19	theme	protective	289:298	arg1	immunity					300:307	a protective immunity	287:307	a protective immunity	287:307	Though they can provide a protective immunity, their multiple side-effects owing to their ill-defined composition and presence of toxic proteins (LF and EF) of Bacillus anthracis, the causative organism of anthrax, in the vaccine formulation makes their widespread use objectionable.
29616046	9	20	dep	I	1860:1860	arg1	C					1862:1862	C	1862:1862	CpG and Poly I:C	1847:1862	Adjuvanation with CpG and Poly I:C, the TMC-PA nanoparticles triggered a Th1 skewed immune response, as suggested by an increase in the levels of total IgG2a along with IFN-γ cytokine production.
29616046	2	21	theme	widespread	517:526	arg1	use					528:530	their widespread use objectionable	511:544	their widespread use objectionable	511:544	Though they can provide a protective immunity, their multiple side-effects owing to their ill-defined composition and presence of toxic proteins (LF and EF) of Bacillus anthracis, the causative organism of anthrax, in the vaccine formulation makes their widespread use objectionable.
29616046	5	22	dep	response	1168:1175	arg1	IgG2a					1223:1227	IgG2a	1223:1227	IgG2a	1223:1227	Irrespective of the route of immunization, CpG or Poly I:C adjuvanted TMC-PA nanoparticles induced a significantly higher humoral response (total serum IgG and its isotypes viz., IgG1, IgG2a, and IgG2b), compared to their CpG or Poly I:C PA counterparts.
29616046	5	22	dep	response	1168:1175	arg1	IgG2b					1234:1238	IgG2b	1234:1238	IgG2b	1234:1238	Irrespective of the route of immunization, CpG or Poly I:C adjuvanted TMC-PA nanoparticles induced a significantly higher humoral response (total serum IgG and its isotypes viz., IgG1, IgG2a, and IgG2b), compared to their CpG or Poly I:C PA counterparts.
29616046	5	22	dep	response	1168:1175	arg1	IgG					1190:1192	total serum IgG	1178:1192	total serum IgG	1178:1192	Irrespective of the route of immunization, CpG or Poly I:C adjuvanted TMC-PA nanoparticles induced a significantly higher humoral response (total serum IgG and its isotypes viz., IgG1, IgG2a, and IgG2b), compared to their CpG or Poly I:C PA counterparts.
29616046	5	22	dep	response	1168:1175	arg1	isotypes					1202:1209	its isotypes	1198:1209	its isotypes viz.	1198:1214	Irrespective of the route of immunization, CpG or Poly I:C adjuvanted TMC-PA nanoparticles induced a significantly higher humoral response (total serum IgG and its isotypes viz., IgG1, IgG2a, and IgG2b), compared to their CpG or Poly I:C PA counterparts.
29616046	5	22	dep	response	1168:1175	arg1	IgG1					1217:1220	IgG1	1217:1220	IgG1	1217:1220	Irrespective of the route of immunization, CpG or Poly I:C adjuvanted TMC-PA nanoparticles induced a significantly higher humoral response (total serum IgG and its isotypes viz., IgG1, IgG2a, and IgG2b), compared to their CpG or Poly I:C PA counterparts.
29616046	9	23	dep	along	1987:1991	arg1	with					1993:1996	with	1993:1996	with	1993:1996	Adjuvanation with CpG and Poly I:C, the TMC-PA nanoparticles triggered a Th1 skewed immune response, as suggested by an increase in the levels of total IgG2a along with IFN-γ cytokine production.
29616046	9	24	theme	immune	1913:1918	arg1	response					1920:1927	a Th1 skewed immune response	1900:1927	a Th1 skewed immune response	1900:1927	Adjuvanation with CpG and Poly I:C, the TMC-PA nanoparticles triggered a Th1 skewed immune response, as suggested by an increase in the levels of total IgG2a along with IFN-γ cytokine production.
29616046	10	25	theme	Th2-biased	2066:2075	arg1	response					2084:2091	a Th2-biased immune response	2064:2091	a Th2-biased immune response	2064:2091	Interestingly, the TMC-PA group showed a Th2-biased immune response.
29616046	9	26	from	increase	1949:1956	arg1	levels					1965:1970	the levels	1961:1970	the levels of total IgG2a	1961:1985	Adjuvanation with CpG and Poly I:C, the TMC-PA nanoparticles triggered a Th1 skewed immune response, as suggested by an increase in the levels of total IgG2a along with IFN-γ cytokine production.
29616046	1	27	theme	deadly	122:127	arg1	disease					129:135	an era old deadly disease	111:135	an era old deadly disease against which there are only two currently available licensed vaccines named anthrax vaccine adsorbed and precipitated (AVP)	111:260	Anthrax is an era old deadly disease against which there are only two currently available licensed vaccines named anthrax vaccine adsorbed and precipitated (AVP).
29616046	1	27	theme	deadly	122:127	arg1	Anthrax					100:106	Anthrax	100:106	Anthrax	100:106	Anthrax is an era old deadly disease against which there are only two currently available licensed vaccines named anthrax vaccine adsorbed and precipitated (AVP).
29616046	12	28	theme	PA-loaded	2341:2349	arg1	candidate					2445:2453	an effective, non-toxic, second generation subunit-vaccine candidate	2386:2453	an effective, non-toxic, second generation subunit-vaccine candidate against anthrax	2386:2469	Altogether, the results suggest that CpG or Poly I:C adjuvanted, PA-loaded TMC nanoparticles could be used as an effective, non-toxic, second generation subunit-vaccine candidate against anthrax.
29616046	12	28	theme	PA-loaded	2341:2349	arg1	nanoparticles					2355:2367	Poly I:C adjuvanted, PA-loaded TMC nanoparticles	2320:2367	nanoparticles	2355:2367	Altogether, the results suggest that CpG or Poly I:C adjuvanted, PA-loaded TMC nanoparticles could be used as an effective, non-toxic, second generation subunit-vaccine candidate against anthrax.
29616046	12	28	theme	PA-loaded	2341:2349	arg1	CpG					2313:2315	CpG	2313:2315	CpG	2313:2315	Altogether, the results suggest that CpG or Poly I:C adjuvanted, PA-loaded TMC nanoparticles could be used as an effective, non-toxic, second generation subunit-vaccine candidate against anthrax.
29616046	11	29	theme	protective	2247:2256	arg1	efficacy					2258:2265	the highest protective efficacy	2235:2265	the highest protective efficacy of ~83%	2235:2273	Upon challenge with the B. anthracis Ames strain, CpG and Poly I:C adjuvanted TMC-PA nanoparticles immunized via the SC and IM routes showed the highest protective efficacy of ~83%.
29616046	11	30	theme	Ames	2131:2134	arg1	strain					2136:2141	the B. anthracis Ames strain	2114:2141	the B. anthracis Ames strain	2114:2141	Upon challenge with the B. anthracis Ames strain, CpG and Poly I:C adjuvanted TMC-PA nanoparticles immunized via the SC and IM routes showed the highest protective efficacy of ~83%.
29616046	4	31	theme	various	705:711	arg1	formulations					721:732	various vaccine formulations	705:732	various vaccine formulations comprising of protective antigen (PA) encapsulated trimethyl-chitosan nanoparticles (TMC-PA) in conjunction with either CpG-C ODN 2395 (CpG) or Poly I:C.	705:886	In this context, we have evaluated the potential of various vaccine formulations comprising of protective antigen (PA) encapsulated trimethyl-chitosan nanoparticles (TMC-PA) in conjunction with either CpG-C ODN 2395 (CpG) or Poly I:C. Each formulation was administered via three different routes, viz., subcutaneous (SC), intramuscular (IM), and intraperitoneal in female BALB/c mice.
29616046	12	32	theme	I	2325:2325	arg1	candidate					2445:2453	an effective, non-toxic, second generation subunit-vaccine candidate	2386:2453	an effective, non-toxic, second generation subunit-vaccine candidate against anthrax	2386:2469	Altogether, the results suggest that CpG or Poly I:C adjuvanted, PA-loaded TMC nanoparticles could be used as an effective, non-toxic, second generation subunit-vaccine candidate against anthrax.
29616046	12	32	theme	I	2325:2325	arg1	nanoparticles					2355:2367	Poly I:C adjuvanted, PA-loaded TMC nanoparticles	2320:2367	nanoparticles	2355:2367	Altogether, the results suggest that CpG or Poly I:C adjuvanted, PA-loaded TMC nanoparticles could be used as an effective, non-toxic, second generation subunit-vaccine candidate against anthrax.
29616046	12	32	theme	I	2325:2325	arg1	CpG					2313:2315	CpG	2313:2315	CpG	2313:2315	Altogether, the results suggest that CpG or Poly I:C adjuvanted, PA-loaded TMC nanoparticles could be used as an effective, non-toxic, second generation subunit-vaccine candidate against anthrax.
29616046	7	33	theme	CpG	1580:1582	arg1	PA					1584:1585	CpG PA	1580:1585	CpG PA	1580:1585	The adjuvant potential of TMC nanoparticles could be observed in all the three routes as the TMC-PA nanoparticles by themselves induced IgG titers (1-1.5 × 105) significantly higher than both CpG PA and Poly I:C PA groups (2-8 × 104).
29616046	11	34	theme	I	2157:2157	arg1	TMC-PA					2172:2177	Poly I:C adjuvanted TMC-PA	2152:2177	Poly I:C adjuvanted TMC-PA	2152:2177	Upon challenge with the B. anthracis Ames strain, CpG and Poly I:C adjuvanted TMC-PA nanoparticles immunized via the SC and IM routes showed the highest protective efficacy of ~83%.
29616046	2	35	theme	anthrax	469:475	arg1	anthracis					432:440	Bacillus anthracis	423:440	Bacillus anthracis	423:440	Though they can provide a protective immunity, their multiple side-effects owing to their ill-defined composition and presence of toxic proteins (LF and EF) of Bacillus anthracis, the causative organism of anthrax, in the vaccine formulation makes their widespread use objectionable.
29616046	2	35	theme	anthrax	469:475	arg1	organism					457:464	the causative organism	443:464	the causative organism of anthrax	443:475	Though they can provide a protective immunity, their multiple side-effects owing to their ill-defined composition and presence of toxic proteins (LF and EF) of Bacillus anthracis, the causative organism of anthrax, in the vaccine formulation makes their widespread use objectionable.
29616046	11	36	theme	TMC-PA	2172:2177	arg1	nanoparticles					2179:2191	CpG and Poly I:C adjuvanted TMC-PA nanoparticles	2144:2191	CpG and Poly I:C adjuvanted TMC-PA nanoparticles immunized via the SC and IM routes	2144:2226	Upon challenge with the B. anthracis Ames strain, CpG and Poly I:C adjuvanted TMC-PA nanoparticles immunized via the SC and IM routes showed the highest protective efficacy of ~83%.
29616046	5	37	theme	total	1178:1182	arg1	IgG2a					1223:1227	IgG2a	1223:1227	IgG2a	1223:1227	Irrespective of the route of immunization, CpG or Poly I:C adjuvanted TMC-PA nanoparticles induced a significantly higher humoral response (total serum IgG and its isotypes viz., IgG1, IgG2a, and IgG2b), compared to their CpG or Poly I:C PA counterparts.
29616046	5	37	theme	total	1178:1182	arg1	IgG					1190:1192	total serum IgG	1178:1192	total serum IgG	1178:1192	Irrespective of the route of immunization, CpG or Poly I:C adjuvanted TMC-PA nanoparticles induced a significantly higher humoral response (total serum IgG and its isotypes viz., IgG1, IgG2a, and IgG2b), compared to their CpG or Poly I:C PA counterparts.
29616046	5	37	theme	total	1178:1182	arg1	IgG2b					1234:1238	IgG2b	1234:1238	IgG2b	1234:1238	Irrespective of the route of immunization, CpG or Poly I:C adjuvanted TMC-PA nanoparticles induced a significantly higher humoral response (total serum IgG and its isotypes viz., IgG1, IgG2a, and IgG2b), compared to their CpG or Poly I:C PA counterparts.
29616046	5	37	theme	total	1178:1182	arg1	IgG1					1217:1220	IgG1	1217:1220	IgG1	1217:1220	Irrespective of the route of immunization, CpG or Poly I:C adjuvanted TMC-PA nanoparticles induced a significantly higher humoral response (total serum IgG and its isotypes viz., IgG1, IgG2a, and IgG2b), compared to their CpG or Poly I:C PA counterparts.
29616046	7	38	theme	nanoparticles	1418:1430	arg1	potential					1401:1409	The adjuvant potential	1388:1409	The adjuvant potential of TMC nanoparticles	1388:1430	The adjuvant potential of TMC nanoparticles could be observed in all the three routes as the TMC-PA nanoparticles by themselves induced IgG titers (1-1.5 × 105) significantly higher than both CpG PA and Poly I:C PA groups (2-8 × 104).
29616046	11	39	theme	C	2159:2159	arg1	TMC-PA					2172:2177	Poly I:C adjuvanted TMC-PA	2152:2177	Poly I:C adjuvanted TMC-PA	2152:2177	Upon challenge with the B. anthracis Ames strain, CpG and Poly I:C adjuvanted TMC-PA nanoparticles immunized via the SC and IM routes showed the highest protective efficacy of ~83%.
29616046	4	40	theme	antigen	759:765	arg1	TMC-PA					819:824	TMC-PA	819:824	TMC-PA	819:824	In this context, we have evaluated the potential of various vaccine formulations comprising of protective antigen (PA) encapsulated trimethyl-chitosan nanoparticles (TMC-PA) in conjunction with either CpG-C ODN 2395 (CpG) or Poly I:C. Each formulation was administered via three different routes, viz., subcutaneous (SC), intramuscular (IM), and intraperitoneal in female BALB/c mice.
29616046	4	40	theme	antigen	759:765	arg1	nanoparticles					804:816	protective antigen (PA) encapsulated trimethyl-chitosan nanoparticles	748:816	protective antigen (PA) encapsulated trimethyl-chitosan nanoparticles (TMC-PA)	748:825	In this context, we have evaluated the potential of various vaccine formulations comprising of protective antigen (PA) encapsulated trimethyl-chitosan nanoparticles (TMC-PA) in conjunction with either CpG-C ODN 2395 (CpG) or Poly I:C. Each formulation was administered via three different routes, viz., subcutaneous (SC), intramuscular (IM), and intraperitoneal in female BALB/c mice.
29616046	11	41	dep	B.	2118:2119	arg1	anthracis					2121:2129	anthracis	2121:2129	anthracis	2121:2129	Upon challenge with the B. anthracis Ames strain, CpG and Poly I:C adjuvanted TMC-PA nanoparticles immunized via the SC and IM routes showed the highest protective efficacy of ~83%.
29616046	5	42	dep	Poly	1267:1270	arg1	counterparts					1279:1290	C PA counterparts	1274:1290	Poly I:C PA counterparts	1267:1290	Irrespective of the route of immunization, CpG or Poly I:C adjuvanted TMC-PA nanoparticles induced a significantly higher humoral response (total serum IgG and its isotypes viz., IgG1, IgG2a, and IgG2b), compared to their CpG or Poly I:C PA counterparts.
29616046	2	43	theme	vaccine	485:491	arg1	formulation					493:503	the vaccine formulation	481:503	the vaccine formulation	481:503	Though they can provide a protective immunity, their multiple side-effects owing to their ill-defined composition and presence of toxic proteins (LF and EF) of Bacillus anthracis, the causative organism of anthrax, in the vaccine formulation makes their widespread use objectionable.
29616046	4	44	theme	PA	768:769	arg1	TMC-PA					819:824	TMC-PA	819:824	TMC-PA	819:824	In this context, we have evaluated the potential of various vaccine formulations comprising of protective antigen (PA) encapsulated trimethyl-chitosan nanoparticles (TMC-PA) in conjunction with either CpG-C ODN 2395 (CpG) or Poly I:C. Each formulation was administered via three different routes, viz., subcutaneous (SC), intramuscular (IM), and intraperitoneal in female BALB/c mice.
29616046	4	44	theme	PA	768:769	arg1	nanoparticles					804:816	protective antigen (PA) encapsulated trimethyl-chitosan nanoparticles	748:816	protective antigen (PA) encapsulated trimethyl-chitosan nanoparticles (TMC-PA)	748:825	In this context, we have evaluated the potential of various vaccine formulations comprising of protective antigen (PA) encapsulated trimethyl-chitosan nanoparticles (TMC-PA) in conjunction with either CpG-C ODN 2395 (CpG) or Poly I:C. Each formulation was administered via three different routes, viz., subcutaneous (SC), intramuscular (IM), and intraperitoneal in female BALB/c mice.
29616046	2	45	theme	proteins	399:406	arg1	presence					381:388	presence	381:388	presence of toxic proteins	381:406	Though they can provide a protective immunity, their multiple side-effects owing to their ill-defined composition and presence of toxic proteins (LF and EF) of Bacillus anthracis, the causative organism of anthrax, in the vaccine formulation makes their widespread use objectionable.
29616046	2	45	theme	proteins	399:406	arg1	composition					365:375	their ill-defined composition	347:375	their ill-defined composition	347:375	Though they can provide a protective immunity, their multiple side-effects owing to their ill-defined composition and presence of toxic proteins (LF and EF) of Bacillus anthracis, the causative organism of anthrax, in the vaccine formulation makes their widespread use objectionable.
29616046	12	46	theme	subunit-vaccine	2429:2443	arg1	candidate					2445:2453	an effective, non-toxic, second generation subunit-vaccine candidate	2386:2453	an effective, non-toxic, second generation subunit-vaccine candidate against anthrax	2386:2469	Altogether, the results suggest that CpG or Poly I:C adjuvanted, PA-loaded TMC nanoparticles could be used as an effective, non-toxic, second generation subunit-vaccine candidate against anthrax.
29616046	12	46	theme	subunit-vaccine	2429:2443	arg1	nanoparticles					2355:2367	Poly I:C adjuvanted, PA-loaded TMC nanoparticles	2320:2367	nanoparticles	2355:2367	Altogether, the results suggest that CpG or Poly I:C adjuvanted, PA-loaded TMC nanoparticles could be used as an effective, non-toxic, second generation subunit-vaccine candidate against anthrax.
29616046	12	46	theme	subunit-vaccine	2429:2443	arg1	CpG					2313:2315	CpG	2313:2315	CpG	2313:2315	Altogether, the results suggest that CpG or Poly I:C adjuvanted, PA-loaded TMC nanoparticles could be used as an effective, non-toxic, second generation subunit-vaccine candidate against anthrax.
29616046	2	47	theme	anthracis	432:440	arg1	presence					381:388	presence	381:388	presence of toxic proteins	381:406	Though they can provide a protective immunity, their multiple side-effects owing to their ill-defined composition and presence of toxic proteins (LF and EF) of Bacillus anthracis, the causative organism of anthrax, in the vaccine formulation makes their widespread use objectionable.
29616046	2	47	theme	anthracis	432:440	arg1	composition					365:375	their ill-defined composition	347:375	their ill-defined composition	347:375	Though they can provide a protective immunity, their multiple side-effects owing to their ill-defined composition and presence of toxic proteins (LF and EF) of Bacillus anthracis, the causative organism of anthrax, in the vaccine formulation makes their widespread use objectionable.
29616046	11	48	theme	B.	2118:2119	arg1	strain					2136:2141	the B. anthracis Ames strain	2114:2141	the B. anthracis Ames strain	2114:2141	Upon challenge with the B. anthracis Ames strain, CpG and Poly I:C adjuvanted TMC-PA nanoparticles immunized via the SC and IM routes showed the highest protective efficacy of ~83%.
29616046	4	49	theme	formulations	721:732	arg1	potential					692:700	the potential	688:700	the potential of various vaccine formulations comprising of protective antigen (PA) encapsulated trimethyl-chitosan nanoparticles (TMC-PA) in conjunction with either CpG-C ODN 2395 (CpG) or Poly I:C. Each formulation was administered via three different routes, viz., subcutaneous (SC), intramuscular (IM), and intraperitoneal in female BALB/c mice	688:1035	In this context, we have evaluated the potential of various vaccine formulations comprising of protective antigen (PA) encapsulated trimethyl-chitosan nanoparticles (TMC-PA) in conjunction with either CpG-C ODN 2395 (CpG) or Poly I:C. Each formulation was administered via three different routes, viz., subcutaneous (SC), intramuscular (IM), and intraperitoneal in female BALB/c mice.
29616046	0	50	theme	Trimethyl	0:8	arg1	Antigen					57:63	Trimethyl Chitosan Nanoparticles Encapsulated Protective Antigen	0:63	Trimethyl Chitosan Nanoparticles Encapsulated Protective Antigen	0:63	Trimethyl Chitosan Nanoparticles Encapsulated Protective Antigen Protects the Mice Against Anthrax.
29616046	5	51	theme	I	1093:1093	arg1	nanoparticles					1115:1127	Poly I:C adjuvanted TMC-PA nanoparticles	1088:1127	Poly I:C adjuvanted TMC-PA nanoparticles	1088:1127	Irrespective of the route of immunization, CpG or Poly I:C adjuvanted TMC-PA nanoparticles induced a significantly higher humoral response (total serum IgG and its isotypes viz., IgG1, IgG2a, and IgG2b), compared to their CpG or Poly I:C PA counterparts.
29616046	9	52	theme	IFN-γ	1998:2002	arg1	production					2013:2022	IFN-γ cytokine production	1998:2022	an increase in the levels of total IgG2a along with IFN-γ cytokine production	1946:2022	Adjuvanation with CpG and Poly I:C, the TMC-PA nanoparticles triggered a Th1 skewed immune response, as suggested by an increase in the levels of total IgG2a along with IFN-γ cytokine production.
29616046	0	53	theme	Nanoparticles	19:31	arg1	Antigen					57:63	Trimethyl Chitosan Nanoparticles Encapsulated Protective Antigen	0:63	Trimethyl Chitosan Nanoparticles Encapsulated Protective Antigen	0:63	Trimethyl Chitosan Nanoparticles Encapsulated Protective Antigen Protects the Mice Against Anthrax.
29616046	5	54	theme	C	1095:1095	arg1	nanoparticles					1115:1127	Poly I:C adjuvanted TMC-PA nanoparticles	1088:1127	Poly I:C adjuvanted TMC-PA nanoparticles	1088:1127	Irrespective of the route of immunization, CpG or Poly I:C adjuvanted TMC-PA nanoparticles induced a significantly higher humoral response (total serum IgG and its isotypes viz., IgG1, IgG2a, and IgG2b), compared to their CpG or Poly I:C PA counterparts.
29616046	9	55	theme	IgG2a	1981:1985	arg1	levels					1965:1970	the levels	1961:1970	the levels of total IgG2a	1961:1985	Adjuvanation with CpG and Poly I:C, the TMC-PA nanoparticles triggered a Th1 skewed immune response, as suggested by an increase in the levels of total IgG2a along with IFN-γ cytokine production.
29616046	4	56	theme	subcutaneous	956:967	arg1	routes					942:947	three different routes	926:947	three different routes	926:947	In this context, we have evaluated the potential of various vaccine formulations comprising of protective antigen (PA) encapsulated trimethyl-chitosan nanoparticles (TMC-PA) in conjunction with either CpG-C ODN 2395 (CpG) or Poly I:C. Each formulation was administered via three different routes, viz., subcutaneous (SC), intramuscular (IM), and intraperitoneal in female BALB/c mice.
29616046	0	57	theme	Protective	46:55	arg1	Antigen					57:63	Trimethyl Chitosan Nanoparticles Encapsulated Protective Antigen	0:63	Trimethyl Chitosan Nanoparticles Encapsulated Protective Antigen	0:63	Trimethyl Chitosan Nanoparticles Encapsulated Protective Antigen Protects the Mice Against Anthrax.
29616046	12	58	theme	generation	2418:2427	arg1	candidate					2445:2453	an effective, non-toxic, second generation subunit-vaccine candidate	2386:2453	an effective, non-toxic, second generation subunit-vaccine candidate against anthrax	2386:2469	Altogether, the results suggest that CpG or Poly I:C adjuvanted, PA-loaded TMC nanoparticles could be used as an effective, non-toxic, second generation subunit-vaccine candidate against anthrax.
29616046	12	58	theme	generation	2418:2427	arg1	nanoparticles					2355:2367	Poly I:C adjuvanted, PA-loaded TMC nanoparticles	2320:2367	nanoparticles	2355:2367	Altogether, the results suggest that CpG or Poly I:C adjuvanted, PA-loaded TMC nanoparticles could be used as an effective, non-toxic, second generation subunit-vaccine candidate against anthrax.
29616046	12	58	theme	generation	2418:2427	arg1	CpG					2313:2315	CpG	2313:2315	CpG	2313:2315	Altogether, the results suggest that CpG or Poly I:C adjuvanted, PA-loaded TMC nanoparticles could be used as an effective, non-toxic, second generation subunit-vaccine candidate against anthrax.
29616046	8	59	dep	Th1-dependant	1716:1728	arg1	cytokines					1818:1826	cytokines	1818:1826	cytokines	1818:1826	The effect of formulations on T-helper (Th) cell development was assessed by quantifying the Th1-dependant (TNF-α, IFN-γ, and IL-2), Th2-dependant (IL-4, IL-6, and IL-10), and Th17-type (IL-17A) cytokines.
29616046	8	59	dep	Th1-dependant	1716:1728	arg1	IFN-γ					1738:1742	IFN-γ	1738:1742	IFN-γ	1738:1742	The effect of formulations on T-helper (Th) cell development was assessed by quantifying the Th1-dependant (TNF-α, IFN-γ, and IL-2), Th2-dependant (IL-4, IL-6, and IL-10), and Th17-type (IL-17A) cytokines.
29616046	8	59	dep	Th1-dependant	1716:1728	arg1	IL-2					1749:1752	IL-2	1749:1752	IL-2	1749:1752	The effect of formulations on T-helper (Th) cell development was assessed by quantifying the Th1-dependant (TNF-α, IFN-γ, and IL-2), Th2-dependant (IL-4, IL-6, and IL-10), and Th17-type (IL-17A) cytokines.
29616046	8	59	dep	Th1-dependant	1716:1728	arg1	TNF-α					1731:1735	TNF-α	1731:1735	TNF-α	1731:1735	The effect of formulations on T-helper (Th) cell development was assessed by quantifying the Th1-dependant (TNF-α, IFN-γ, and IL-2), Th2-dependant (IL-4, IL-6, and IL-10), and Th17-type (IL-17A) cytokines.
29616046	4	60	theme	encapsulated	772:783	arg1	TMC-PA					819:824	TMC-PA	819:824	TMC-PA	819:824	In this context, we have evaluated the potential of various vaccine formulations comprising of protective antigen (PA) encapsulated trimethyl-chitosan nanoparticles (TMC-PA) in conjunction with either CpG-C ODN 2395 (CpG) or Poly I:C. Each formulation was administered via three different routes, viz., subcutaneous (SC), intramuscular (IM), and intraperitoneal in female BALB/c mice.
29616046	4	60	theme	encapsulated	772:783	arg1	nanoparticles					804:816	protective antigen (PA) encapsulated trimethyl-chitosan nanoparticles	748:816	protective antigen (PA) encapsulated trimethyl-chitosan nanoparticles (TMC-PA)	748:825	In this context, we have evaluated the potential of various vaccine formulations comprising of protective antigen (PA) encapsulated trimethyl-chitosan nanoparticles (TMC-PA) in conjunction with either CpG-C ODN 2395 (CpG) or Poly I:C. Each formulation was administered via three different routes, viz., subcutaneous (SC), intramuscular (IM), and intraperitoneal in female BALB/c mice.
29616046	9	61	theme	total	1975:1979	arg1	IgG2a					1981:1985	total IgG2a	1975:1985	total IgG2a	1975:1985	Adjuvanation with CpG and Poly I:C, the TMC-PA nanoparticles triggered a Th1 skewed immune response, as suggested by an increase in the levels of total IgG2a along with IFN-γ cytokine production.
29616046	6	62	theme	Poly	1355:1358	arg1	I					1360:1360	Poly I	1355:1360	Poly I:C	1355:1362	This clearly demonstrates the synergistic behavior of CpG and Poly I:C with TMC nanoparticles.
29616046	2	63	attach	presence	381:388	arg2	organism					457:464	the causative organism	443:464	the causative organism of anthrax	443:475	Though they can provide a protective immunity, their multiple side-effects owing to their ill-defined composition and presence of toxic proteins (LF and EF) of Bacillus anthracis, the causative organism of anthrax, in the vaccine formulation makes their widespread use objectionable.
29616046	2	63	attach	presence	381:388	arg1	formulation					493:503	the vaccine formulation	481:503	the vaccine formulation	481:503	Though they can provide a protective immunity, their multiple side-effects owing to their ill-defined composition and presence of toxic proteins (LF and EF) of Bacillus anthracis, the causative organism of anthrax, in the vaccine formulation makes their widespread use objectionable.
29616046	2	63	attach	presence	381:388	arg2	anthracis					432:440	Bacillus anthracis	423:440	Bacillus anthracis	423:440	Though they can provide a protective immunity, their multiple side-effects owing to their ill-defined composition and presence of toxic proteins (LF and EF) of Bacillus anthracis, the causative organism of anthrax, in the vaccine formulation makes their widespread use objectionable.
29616046	2	63	attach	presence	381:388	arg2	proteins					399:406	toxic proteins	393:406	toxic proteins	393:406	Though they can provide a protective immunity, their multiple side-effects owing to their ill-defined composition and presence of toxic proteins (LF and EF) of Bacillus anthracis, the causative organism of anthrax, in the vaccine formulation makes their widespread use objectionable.
29616046	7	64	located	observed	1441:1448	arg2	potential					1401:1409	The adjuvant potential	1388:1409	The adjuvant potential of TMC nanoparticles	1388:1430	The adjuvant potential of TMC nanoparticles could be observed in all the three routes as the TMC-PA nanoparticles by themselves induced IgG titers (1-1.5 × 105) significantly higher than both CpG PA and Poly I:C PA groups (2-8 × 104).
29616046	7	64	located	observed	1441:1448	arg1	routes					1467:1472	all the three routes	1453:1472	all the three routes as the TMC-PA nanoparticles	1453:1500	The adjuvant potential of TMC nanoparticles could be observed in all the three routes as the TMC-PA nanoparticles by themselves induced IgG titers (1-1.5 × 105) significantly higher than both CpG PA and Poly I:C PA groups (2-8 × 104).
29616046	4	65	theme	BALB/c	1025:1030	arg1	mice					1032:1035	female BALB/c mice	1018:1035	female BALB/c mice	1018:1035	In this context, we have evaluated the potential of various vaccine formulations comprising of protective antigen (PA) encapsulated trimethyl-chitosan nanoparticles (TMC-PA) in conjunction with either CpG-C ODN 2395 (CpG) or Poly I:C. Each formulation was administered via three different routes, viz., subcutaneous (SC), intramuscular (IM), and intraperitoneal in female BALB/c mice.
29616046	11	66	theme	%	2273:2273	arg1	efficacy					2258:2265	the highest protective efficacy	2235:2265	the highest protective efficacy of ~83%	2235:2273	Upon challenge with the B. anthracis Ames strain, CpG and Poly I:C adjuvanted TMC-PA nanoparticles immunized via the SC and IM routes showed the highest protective efficacy of ~83%.
29616046	6	67	theme	CpG	1347:1349	arg1	behavior					1335:1342	the synergistic behavior	1319:1342	the synergistic behavior of CpG and Poly I:C with TMC nanoparticles	1319:1385	This clearly demonstrates the synergistic behavior of CpG and Poly I:C with TMC nanoparticles.
29616046	5	68	theme	humoral	1160:1166	arg1	response					1168:1175	a significantly higher humoral response	1137:1175	a significantly higher humoral response (total serum IgG and its isotypes viz., IgG1, IgG2a, and IgG2b)	1137:1239	Irrespective of the route of immunization, CpG or Poly I:C adjuvanted TMC-PA nanoparticles induced a significantly higher humoral response (total serum IgG and its isotypes viz., IgG1, IgG2a, and IgG2b), compared to their CpG or Poly I:C PA counterparts.
29616046	6	69	dep	I	1360:1360	arg1	C					1362:1362	C	1362:1362	Poly I:C	1355:1362	This clearly demonstrates the synergistic behavior of CpG and Poly I:C with TMC nanoparticles.
29616046	2	70	theme	ill-defined	353:363	arg1	composition					365:375	their ill-defined composition	347:375	their ill-defined composition	347:375	Though they can provide a protective immunity, their multiple side-effects owing to their ill-defined composition and presence of toxic proteins (LF and EF) of Bacillus anthracis, the causative organism of anthrax, in the vaccine formulation makes their widespread use objectionable.
29616046	5	71	theme	TMC-PA	1108:1113	arg1	nanoparticles					1115:1127	Poly I:C adjuvanted TMC-PA nanoparticles	1088:1127	Poly I:C adjuvanted TMC-PA nanoparticles	1088:1127	Irrespective of the route of immunization, CpG or Poly I:C adjuvanted TMC-PA nanoparticles induced a significantly higher humoral response (total serum IgG and its isotypes viz., IgG1, IgG2a, and IgG2b), compared to their CpG or Poly I:C PA counterparts.
29616046	12	72	theme	effective	2389:2397	arg1	candidate					2445:2453	an effective, non-toxic, second generation subunit-vaccine candidate	2386:2453	an effective, non-toxic, second generation subunit-vaccine candidate against anthrax	2386:2469	Altogether, the results suggest that CpG or Poly I:C adjuvanted, PA-loaded TMC nanoparticles could be used as an effective, non-toxic, second generation subunit-vaccine candidate against anthrax.
29616046	12	72	theme	effective	2389:2397	arg1	nanoparticles					2355:2367	Poly I:C adjuvanted, PA-loaded TMC nanoparticles	2320:2367	nanoparticles	2355:2367	Altogether, the results suggest that CpG or Poly I:C adjuvanted, PA-loaded TMC nanoparticles could be used as an effective, non-toxic, second generation subunit-vaccine candidate against anthrax.
29616046	12	72	theme	effective	2389:2397	arg1	CpG					2313:2315	CpG	2313:2315	CpG	2313:2315	Altogether, the results suggest that CpG or Poly I:C adjuvanted, PA-loaded TMC nanoparticles could be used as an effective, non-toxic, second generation subunit-vaccine candidate against anthrax.
29616046	4	73	dep	subcutaneous	956:967	arg1	SC					970:971	SC	970:971	SC	970:971	In this context, we have evaluated the potential of various vaccine formulations comprising of protective antigen (PA) encapsulated trimethyl-chitosan nanoparticles (TMC-PA) in conjunction with either CpG-C ODN 2395 (CpG) or Poly I:C. Each formulation was administered via three different routes, viz., subcutaneous (SC), intramuscular (IM), and intraperitoneal in female BALB/c mice.
29616046	12	74	used	used	2378:2381	arg2	nanoparticles					2355:2367	Poly I:C adjuvanted, PA-loaded TMC nanoparticles	2320:2367	nanoparticles	2355:2367	Altogether, the results suggest that CpG or Poly I:C adjuvanted, PA-loaded TMC nanoparticles could be used as an effective, non-toxic, second generation subunit-vaccine candidate against anthrax.
29616046	12	74	used	used	2378:2381	arg2	CpG					2313:2315	CpG	2313:2315	CpG	2313:2315	Altogether, the results suggest that CpG or Poly I:C adjuvanted, PA-loaded TMC nanoparticles could be used as an effective, non-toxic, second generation subunit-vaccine candidate against anthrax.
29616046	12	74	used	used	2378:2381	arg2	candidate					2445:2453	an effective, non-toxic, second generation subunit-vaccine candidate	2386:2453	an effective, non-toxic, second generation subunit-vaccine candidate against anthrax	2386:2469	Altogether, the results suggest that CpG or Poly I:C adjuvanted, PA-loaded TMC nanoparticles could be used as an effective, non-toxic, second generation subunit-vaccine candidate against anthrax.
29616046	7	75	theme	C	1598:1598	arg1	PA					1600:1601	Poly I:C PA	1591:1601	Poly I:C PA	1591:1601	The adjuvant potential of TMC nanoparticles could be observed in all the three routes as the TMC-PA nanoparticles by themselves induced IgG titers (1-1.5 × 105) significantly higher than both CpG PA and Poly I:C PA groups (2-8 × 104).
29616046	9	76	with	Adjuvanation	1829:1840	arg1	I					1860:1860	CpG and Poly I	1847:1860	I	1860:1860	Adjuvanation with CpG and Poly I:C, the TMC-PA nanoparticles triggered a Th1 skewed immune response, as suggested by an increase in the levels of total IgG2a along with IFN-γ cytokine production.
29616046	9	76	with	Adjuvanation	1829:1840	arg1	nanoparticles					1876:1888	the TMC-PA nanoparticles	1865:1888	the TMC-PA nanoparticles	1865:1888	Adjuvanation with CpG and Poly I:C, the TMC-PA nanoparticles triggered a Th1 skewed immune response, as suggested by an increase in the levels of total IgG2a along with IFN-γ cytokine production.
29616046	5	77	theme	C	1274:1274	arg1	counterparts					1279:1290	C PA counterparts	1274:1290	Poly I:C PA counterparts	1267:1290	Irrespective of the route of immunization, CpG or Poly I:C adjuvanted TMC-PA nanoparticles induced a significantly higher humoral response (total serum IgG and its isotypes viz., IgG1, IgG2a, and IgG2b), compared to their CpG or Poly I:C PA counterparts.
29616046	1	78	theme	available	180:188	arg1	vaccines					199:206	two currently available licensed vaccines	166:206	two currently available licensed vaccines named anthrax vaccine	166:228	Anthrax is an era old deadly disease against which there are only two currently available licensed vaccines named anthrax vaccine adsorbed and precipitated (AVP).
29616046	7	79	theme	TMC-PA	1481:1486	arg1	nanoparticles					1488:1500	the TMC-PA nanoparticles	1477:1500	the TMC-PA nanoparticles	1477:1500	The adjuvant potential of TMC nanoparticles could be observed in all the three routes as the TMC-PA nanoparticles by themselves induced IgG titers (1-1.5 × 105) significantly higher than both CpG PA and Poly I:C PA groups (2-8 × 104).
29616046	7	80	theme	I	1596:1596	arg1	PA					1600:1601	Poly I:C PA	1591:1601	Poly I:C PA	1591:1601	The adjuvant potential of TMC nanoparticles could be observed in all the three routes as the TMC-PA nanoparticles by themselves induced IgG titers (1-1.5 × 105) significantly higher than both CpG PA and Poly I:C PA groups (2-8 × 104).
29616046	12	81	theme	TMC	2351:2353	arg1	candidate					2445:2453	an effective, non-toxic, second generation subunit-vaccine candidate	2386:2453	an effective, non-toxic, second generation subunit-vaccine candidate against anthrax	2386:2469	Altogether, the results suggest that CpG or Poly I:C adjuvanted, PA-loaded TMC nanoparticles could be used as an effective, non-toxic, second generation subunit-vaccine candidate against anthrax.
29616046	12	81	theme	TMC	2351:2353	arg1	nanoparticles					2355:2367	Poly I:C adjuvanted, PA-loaded TMC nanoparticles	2320:2367	nanoparticles	2355:2367	Altogether, the results suggest that CpG or Poly I:C adjuvanted, PA-loaded TMC nanoparticles could be used as an effective, non-toxic, second generation subunit-vaccine candidate against anthrax.
29616046	12	81	theme	TMC	2351:2353	arg1	CpG					2313:2315	CpG	2313:2315	CpG	2313:2315	Altogether, the results suggest that CpG or Poly I:C adjuvanted, PA-loaded TMC nanoparticles could be used as an effective, non-toxic, second generation subunit-vaccine candidate against anthrax.
29616046	1	82	dep	precipitated	243:254	arg1	AVP					257:259	AVP	257:259	AVP	257:259	Anthrax is an era old deadly disease against which there are only two currently available licensed vaccines named anthrax vaccine adsorbed and precipitated (AVP).
29616046	9	83	theme	skewed	1906:1911	arg1	response					1920:1927	a Th1 skewed immune response	1900:1927	a Th1 skewed immune response	1900:1927	Adjuvanation with CpG and Poly I:C, the TMC-PA nanoparticles triggered a Th1 skewed immune response, as suggested by an increase in the levels of total IgG2a along with IFN-γ cytokine production.
29616046	1	84	theme	anthrax	214:220	arg1	vaccine					222:228	anthrax vaccine	214:228	anthrax vaccine	214:228	Anthrax is an era old deadly disease against which there are only two currently available licensed vaccines named anthrax vaccine adsorbed and precipitated (AVP).
29616046	11	85	theme	SC	2211:2212	arg1	routes					2221:2226	the SC and IM routes	2207:2226	routes	2221:2226	Upon challenge with the B. anthracis Ames strain, CpG and Poly I:C adjuvanted TMC-PA nanoparticles immunized via the SC and IM routes showed the highest protective efficacy of ~83%.
29616046	12	86	theme	Poly	2320:2323	arg1	candidate					2445:2453	an effective, non-toxic, second generation subunit-vaccine candidate	2386:2453	an effective, non-toxic, second generation subunit-vaccine candidate against anthrax	2386:2469	Altogether, the results suggest that CpG or Poly I:C adjuvanted, PA-loaded TMC nanoparticles could be used as an effective, non-toxic, second generation subunit-vaccine candidate against anthrax.
29616046	12	86	theme	Poly	2320:2323	arg1	nanoparticles					2355:2367	Poly I:C adjuvanted, PA-loaded TMC nanoparticles	2320:2367	nanoparticles	2355:2367	Altogether, the results suggest that CpG or Poly I:C adjuvanted, PA-loaded TMC nanoparticles could be used as an effective, non-toxic, second generation subunit-vaccine candidate against anthrax.
29616046	12	86	theme	Poly	2320:2323	arg1	CpG					2313:2315	CpG	2313:2315	CpG	2313:2315	Altogether, the results suggest that CpG or Poly I:C adjuvanted, PA-loaded TMC nanoparticles could be used as an effective, non-toxic, second generation subunit-vaccine candidate against anthrax.
29616046	3	87	theme	anthrax	557:563	arg1	desirable					642:650	desirable	642:650	desirable	642:650	Hence, an anthrax vaccine that contains well-defined and controlled components would be highly desirable.
29616046	3	87	theme	anthrax	557:563	arg1	vaccine					565:571	an anthrax vaccine	554:571	an anthrax vaccine that contains well-defined and controlled components	554:624	Hence, an anthrax vaccine that contains well-defined and controlled components would be highly desirable.
29616046	1	88	theme	old	118:120	arg1	disease					129:135	an era old deadly disease	111:135	an era old deadly disease against which there are only two currently available licensed vaccines named anthrax vaccine adsorbed and precipitated (AVP)	111:260	Anthrax is an era old deadly disease against which there are only two currently available licensed vaccines named anthrax vaccine adsorbed and precipitated (AVP).
29616046	1	88	theme	old	118:120	arg1	Anthrax					100:106	Anthrax	100:106	Anthrax	100:106	Anthrax is an era old deadly disease against which there are only two currently available licensed vaccines named anthrax vaccine adsorbed and precipitated (AVP).
29616046	10	89	theme	TMC-PA	2044:2049	arg1	group					2051:2055	the TMC-PA group	2040:2055	the TMC-PA group	2040:2055	Interestingly, the TMC-PA group showed a Th2-biased immune response.
29616046	11	90	theme	highest	2239:2245	arg1	efficacy					2258:2265	the highest protective efficacy	2235:2265	the highest protective efficacy of ~83%	2235:2273	Upon challenge with the B. anthracis Ames strain, CpG and Poly I:C adjuvanted TMC-PA nanoparticles immunized via the SC and IM routes showed the highest protective efficacy of ~83%.
29616046	8	91	from	effect	1627:1632	arg1	development					1672:1682	T-helper (Th) cell development	1653:1682	T-helper (Th) cell development	1653:1682	The effect of formulations on T-helper (Th) cell development was assessed by quantifying the Th1-dependant (TNF-α, IFN-γ, and IL-2), Th2-dependant (IL-4, IL-6, and IL-10), and Th17-type (IL-17A) cytokines.
29616046	3	92	theme	controlled	604:613	arg1	components					615:624	well-defined and controlled components	587:624	well-defined and controlled components	587:624	Hence, an anthrax vaccine that contains well-defined and controlled components would be highly desirable.
29616046	4	93	dep	formulations	721:732	arg1	C.					885:886	C.	885:886	C.	885:886	In this context, we have evaluated the potential of various vaccine formulations comprising of protective antigen (PA) encapsulated trimethyl-chitosan nanoparticles (TMC-PA) in conjunction with either CpG-C ODN 2395 (CpG) or Poly I:C. Each formulation was administered via three different routes, viz., subcutaneous (SC), intramuscular (IM), and intraperitoneal in female BALB/c mice.
29616046	7	94	theme	Poly	1591:1594	arg1	PA					1600:1601	Poly I:C PA	1591:1601	Poly I:C PA	1591:1601	The adjuvant potential of TMC nanoparticles could be observed in all the three routes as the TMC-PA nanoparticles by themselves induced IgG titers (1-1.5 × 105) significantly higher than both CpG PA and Poly I:C PA groups (2-8 × 104).
29616046	4	95	theme	Poly	878:881	arg1	I					883:883	Poly I	878:883	Poly I	878:883	In this context, we have evaluated the potential of various vaccine formulations comprising of protective antigen (PA) encapsulated trimethyl-chitosan nanoparticles (TMC-PA) in conjunction with either CpG-C ODN 2395 (CpG) or Poly I:C. Each formulation was administered via three different routes, viz., subcutaneous (SC), intramuscular (IM), and intraperitoneal in female BALB/c mice.
29616046	11	96	theme	Poly	2152:2155	arg1	TMC-PA					2172:2177	Poly I:C adjuvanted TMC-PA	2152:2177	Poly I:C adjuvanted TMC-PA	2152:2177	Upon challenge with the B. anthracis Ames strain, CpG and Poly I:C adjuvanted TMC-PA nanoparticles immunized via the SC and IM routes showed the highest protective efficacy of ~83%.
29616046	7	97	theme	higher	1563:1568	arg1	1-1.5 × 105					1536:1546	1-1.5 × 105	1536:1546	1-1.5 × 105	1536:1546	The adjuvant potential of TMC nanoparticles could be observed in all the three routes as the TMC-PA nanoparticles by themselves induced IgG titers (1-1.5 × 105) significantly higher than both CpG PA and Poly I:C PA groups (2-8 × 104).
29616046	7	97	theme	higher	1563:1568	arg1	titers					1528:1533	IgG titers	1524:1533	IgG titers (1-1.5 × 105) significantly higher than both CpG PA and Poly I:C PA groups (2-8 × 104)	1524:1620	The adjuvant potential of TMC nanoparticles could be observed in all the three routes as the TMC-PA nanoparticles by themselves induced IgG titers (1-1.5 × 105) significantly higher than both CpG PA and Poly I:C PA groups (2-8 × 104).
29616046	3	98	theme	well-defined	587:598	arg1	components					615:624	well-defined and controlled components	587:624	well-defined and controlled components	587:624	Hence, an anthrax vaccine that contains well-defined and controlled components would be highly desirable.
29616046	7	99	theme	PA	1584:1585	arg1	2-8 × 104					1611:1619	2-8 × 104	1611:1619	2-8 × 104	1611:1619	The adjuvant potential of TMC nanoparticles could be observed in all the three routes as the TMC-PA nanoparticles by themselves induced IgG titers (1-1.5 × 105) significantly higher than both CpG PA and Poly I:C PA groups (2-8 × 104).
29616046	7	99	theme	PA	1584:1585	arg1	groups					1603:1608	both CpG PA and Poly I:C PA groups	1575:1608	both CpG PA and Poly I:C PA groups (2-8 × 104)	1575:1620	The adjuvant potential of TMC nanoparticles could be observed in all the three routes as the TMC-PA nanoparticles by themselves induced IgG titers (1-1.5 × 105) significantly higher than both CpG PA and Poly I:C PA groups (2-8 × 104).
29616046	4	100	theme	different	932:940	arg1	routes					942:947	three different routes	926:947	three different routes	926:947	In this context, we have evaluated the potential of various vaccine formulations comprising of protective antigen (PA) encapsulated trimethyl-chitosan nanoparticles (TMC-PA) in conjunction with either CpG-C ODN 2395 (CpG) or Poly I:C. Each formulation was administered via three different routes, viz., subcutaneous (SC), intramuscular (IM), and intraperitoneal in female BALB/c mice.
29616046	11	101	theme	adjuvanted	2161:2170	arg1	TMC-PA					2172:2177	Poly I:C adjuvanted TMC-PA	2152:2177	Poly I:C adjuvanted TMC-PA	2152:2177	Upon challenge with the B. anthracis Ames strain, CpG and Poly I:C adjuvanted TMC-PA nanoparticles immunized via the SC and IM routes showed the highest protective efficacy of ~83%.
29616046	11	102	theme	CpG	2144:2146	arg1	nanoparticles					2179:2191	CpG and Poly I:C adjuvanted TMC-PA nanoparticles	2144:2191	CpG and Poly I:C adjuvanted TMC-PA nanoparticles immunized via the SC and IM routes	2144:2226	Upon challenge with the B. anthracis Ames strain, CpG and Poly I:C adjuvanted TMC-PA nanoparticles immunized via the SC and IM routes showed the highest protective efficacy of ~83%.
29616046	5	103	theme	route	1058:1062	arg1	Irrespective					1038:1049	Irrespective	1038:1049	Irrespective	1038:1049	Irrespective of the route of immunization, CpG or Poly I:C adjuvanted TMC-PA nanoparticles induced a significantly higher humoral response (total serum IgG and its isotypes viz., IgG1, IgG2a, and IgG2b), compared to their CpG or Poly I:C PA counterparts.
29616046	7	104	theme	TMC	1414:1416	arg1	nanoparticles					1418:1430	TMC nanoparticles	1414:1430	TMC nanoparticles	1414:1430	The adjuvant potential of TMC nanoparticles could be observed in all the three routes as the TMC-PA nanoparticles by themselves induced IgG titers (1-1.5 × 105) significantly higher than both CpG PA and Poly I:C PA groups (2-8 × 104).
29616046	10	105	theme	immune	2077:2082	arg1	response					2084:2091	a Th2-biased immune response	2064:2091	a Th2-biased immune response	2064:2091	Interestingly, the TMC-PA group showed a Th2-biased immune response.
29616046	11	106	with	challenge	2099:2107	arg1	strain					2136:2141	the B. anthracis Ames strain	2114:2141	the B. anthracis Ames strain	2114:2141	Upon challenge with the B. anthracis Ames strain, CpG and Poly I:C adjuvanted TMC-PA nanoparticles immunized via the SC and IM routes showed the highest protective efficacy of ~83%.
29616046	5	107	theme	serum	1184:1188	arg1	IgG2a					1223:1227	IgG2a	1223:1227	IgG2a	1223:1227	Irrespective of the route of immunization, CpG or Poly I:C adjuvanted TMC-PA nanoparticles induced a significantly higher humoral response (total serum IgG and its isotypes viz., IgG1, IgG2a, and IgG2b), compared to their CpG or Poly I:C PA counterparts.
29616046	5	107	theme	serum	1184:1188	arg1	IgG					1190:1192	total serum IgG	1178:1192	total serum IgG	1178:1192	Irrespective of the route of immunization, CpG or Poly I:C adjuvanted TMC-PA nanoparticles induced a significantly higher humoral response (total serum IgG and its isotypes viz., IgG1, IgG2a, and IgG2b), compared to their CpG or Poly I:C PA counterparts.
29616046	5	107	theme	serum	1184:1188	arg1	IgG2b					1234:1238	IgG2b	1234:1238	IgG2b	1234:1238	Irrespective of the route of immunization, CpG or Poly I:C adjuvanted TMC-PA nanoparticles induced a significantly higher humoral response (total serum IgG and its isotypes viz., IgG1, IgG2a, and IgG2b), compared to their CpG or Poly I:C PA counterparts.
29616046	5	107	theme	serum	1184:1188	arg1	IgG1					1217:1220	IgG1	1217:1220	IgG1	1217:1220	Irrespective of the route of immunization, CpG or Poly I:C adjuvanted TMC-PA nanoparticles induced a significantly higher humoral response (total serum IgG and its isotypes viz., IgG1, IgG2a, and IgG2b), compared to their CpG or Poly I:C PA counterparts.
29616046	11	108	theme	IM	2218:2219	arg1	routes					2221:2226	the SC and IM routes	2207:2226	routes	2221:2226	Upon challenge with the B. anthracis Ames strain, CpG and Poly I:C adjuvanted TMC-PA nanoparticles immunized via the SC and IM routes showed the highest protective efficacy of ~83%.
29616046	5	109	theme	immunization	1067:1078	arg1	route					1058:1062	the route	1054:1062	the route of immunization	1054:1078	Irrespective of the route of immunization, CpG or Poly I:C adjuvanted TMC-PA nanoparticles induced a significantly higher humoral response (total serum IgG and its isotypes viz., IgG1, IgG2a, and IgG2b), compared to their CpG or Poly I:C PA counterparts.
29616046	4	110	with	conjunction	830:840	arg1	I					883:883	Poly I	878:883	Poly I	878:883	In this context, we have evaluated the potential of various vaccine formulations comprising of protective antigen (PA) encapsulated trimethyl-chitosan nanoparticles (TMC-PA) in conjunction with either CpG-C ODN 2395 (CpG) or Poly I:C. Each formulation was administered via three different routes, viz., subcutaneous (SC), intramuscular (IM), and intraperitoneal in female BALB/c mice.
29616046	4	110	with	conjunction	830:840	arg1	ODN					860:862	CpG-C ODN 2395	854:867	CpG-C ODN 2395 (CpG)	854:873	In this context, we have evaluated the potential of various vaccine formulations comprising of protective antigen (PA) encapsulated trimethyl-chitosan nanoparticles (TMC-PA) in conjunction with either CpG-C ODN 2395 (CpG) or Poly I:C. Each formulation was administered via three different routes, viz., subcutaneous (SC), intramuscular (IM), and intraperitoneal in female BALB/c mice.
29616046	4	110	with	conjunction	830:840	arg1	CpG					870:872	CpG	870:872	CpG	870:872	In this context, we have evaluated the potential of various vaccine formulations comprising of protective antigen (PA) encapsulated trimethyl-chitosan nanoparticles (TMC-PA) in conjunction with either CpG-C ODN 2395 (CpG) or Poly I:C. Each formulation was administered via three different routes, viz., subcutaneous (SC), intramuscular (IM), and intraperitoneal in female BALB/c mice.
29616046	4	111	theme	protective	748:757	arg1	TMC-PA					819:824	TMC-PA	819:824	TMC-PA	819:824	In this context, we have evaluated the potential of various vaccine formulations comprising of protective antigen (PA) encapsulated trimethyl-chitosan nanoparticles (TMC-PA) in conjunction with either CpG-C ODN 2395 (CpG) or Poly I:C. Each formulation was administered via three different routes, viz., subcutaneous (SC), intramuscular (IM), and intraperitoneal in female BALB/c mice.
29616046	4	111	theme	protective	748:757	arg1	nanoparticles					804:816	protective antigen (PA) encapsulated trimethyl-chitosan nanoparticles	748:816	protective antigen (PA) encapsulated trimethyl-chitosan nanoparticles (TMC-PA)	748:825	In this context, we have evaluated the potential of various vaccine formulations comprising of protective antigen (PA) encapsulated trimethyl-chitosan nanoparticles (TMC-PA) in conjunction with either CpG-C ODN 2395 (CpG) or Poly I:C. Each formulation was administered via three different routes, viz., subcutaneous (SC), intramuscular (IM), and intraperitoneal in female BALB/c mice.
29616046	2	112	dep	composition	365:375	arg1	LF					409:410	LF	409:410	LF	409:410	Though they can provide a protective immunity, their multiple side-effects owing to their ill-defined composition and presence of toxic proteins (LF and EF) of Bacillus anthracis, the causative organism of anthrax, in the vaccine formulation makes their widespread use objectionable.
29616046	2	112	dep	composition	365:375	arg1	EF					416:417	EF	416:417	EF	416:417	Though they can provide a protective immunity, their multiple side-effects owing to their ill-defined composition and presence of toxic proteins (LF and EF) of Bacillus anthracis, the causative organism of anthrax, in the vaccine formulation makes their widespread use objectionable.
29616046	2	113	theme	toxic	393:397	arg1	proteins					399:406	toxic proteins	393:406	toxic proteins	393:406	Though they can provide a protective immunity, their multiple side-effects owing to their ill-defined composition and presence of toxic proteins (LF and EF) of Bacillus anthracis, the causative organism of anthrax, in the vaccine formulation makes their widespread use objectionable.
29616046	0	114	theme	Chitosan	10:17	arg1	Antigen					57:63	Trimethyl Chitosan Nanoparticles Encapsulated Protective Antigen	0:63	Trimethyl Chitosan Nanoparticles Encapsulated Protective Antigen	0:63	Trimethyl Chitosan Nanoparticles Encapsulated Protective Antigen Protects the Mice Against Anthrax.
29616046	8	115	theme	formulations	1637:1648	arg1	effect					1627:1632	The effect	1623:1632	The effect of formulations on T-helper (Th) cell development	1623:1682	The effect of formulations on T-helper (Th) cell development was assessed by quantifying the Th1-dependant (TNF-α, IFN-γ, and IL-2), Th2-dependant (IL-4, IL-6, and IL-10), and Th17-type (IL-17A) cytokines.
29616046	4	116	theme	vaccine	713:719	arg1	formulations					721:732	various vaccine formulations	705:732	various vaccine formulations comprising of protective antigen (PA) encapsulated trimethyl-chitosan nanoparticles (TMC-PA) in conjunction with either CpG-C ODN 2395 (CpG) or Poly I:C.	705:886	In this context, we have evaluated the potential of various vaccine formulations comprising of protective antigen (PA) encapsulated trimethyl-chitosan nanoparticles (TMC-PA) in conjunction with either CpG-C ODN 2395 (CpG) or Poly I:C. Each formulation was administered via three different routes, viz., subcutaneous (SC), intramuscular (IM), and intraperitoneal in female BALB/c mice.
29616046	0	117	theme	Encapsulated	33:44	arg1	Antigen					57:63	Trimethyl Chitosan Nanoparticles Encapsulated Protective Antigen	0:63	Trimethyl Chitosan Nanoparticles Encapsulated Protective Antigen	0:63	Trimethyl Chitosan Nanoparticles Encapsulated Protective Antigen Protects the Mice Against Anthrax.
29616046	8	118	theme	T-helper	1653:1660	arg1	development					1672:1682	T-helper (Th) cell development	1653:1682	T-helper (Th) cell development	1653:1682	The effect of formulations on T-helper (Th) cell development was assessed by quantifying the Th1-dependant (TNF-α, IFN-γ, and IL-2), Th2-dependant (IL-4, IL-6, and IL-10), and Th17-type (IL-17A) cytokines.
29616046	4	119	dep	potential	692:700	arg1	administered					909:920	administered	909:920	was administered via three different routes, viz., subcutaneous (SC), intramuscular (IM), and intraperitoneal in female BALB/c mice	905:1035	In this context, we have evaluated the potential of various vaccine formulations comprising of protective antigen (PA) encapsulated trimethyl-chitosan nanoparticles (TMC-PA) in conjunction with either CpG-C ODN 2395 (CpG) or Poly I:C. Each formulation was administered via three different routes, viz., subcutaneous (SC), intramuscular (IM), and intraperitoneal in female BALB/c mice.
29616046	7	120	theme	IgG	1524:1526	arg1	1-1.5 × 105					1536:1546	1-1.5 × 105	1536:1546	1-1.5 × 105	1536:1546	The adjuvant potential of TMC nanoparticles could be observed in all the three routes as the TMC-PA nanoparticles by themselves induced IgG titers (1-1.5 × 105) significantly higher than both CpG PA and Poly I:C PA groups (2-8 × 104).
29616046	7	120	theme	IgG	1524:1526	arg1	titers					1528:1533	IgG titers	1524:1533	IgG titers (1-1.5 × 105) significantly higher than both CpG PA and Poly I:C PA groups (2-8 × 104)	1524:1620	The adjuvant potential of TMC nanoparticles could be observed in all the three routes as the TMC-PA nanoparticles by themselves induced IgG titers (1-1.5 × 105) significantly higher than both CpG PA and Poly I:C PA groups (2-8 × 104).
29616046	4	121	theme	intramuscular	975:987	arg1	routes					942:947	three different routes	926:947	three different routes	926:947	In this context, we have evaluated the potential of various vaccine formulations comprising of protective antigen (PA) encapsulated trimethyl-chitosan nanoparticles (TMC-PA) in conjunction with either CpG-C ODN 2395 (CpG) or Poly I:C. Each formulation was administered via three different routes, viz., subcutaneous (SC), intramuscular (IM), and intraperitoneal in female BALB/c mice.
29616046	9	122	theme	cytokine	2004:2011	arg1	production					2013:2022	IFN-γ cytokine production	1998:2022	an increase in the levels of total IgG2a along with IFN-γ cytokine production	1946:2022	Adjuvanation with CpG and Poly I:C, the TMC-PA nanoparticles triggered a Th1 skewed immune response, as suggested by an increase in the levels of total IgG2a along with IFN-γ cytokine production.
29616046	5	123	theme	adjuvanted	1097:1106	arg1	nanoparticles					1115:1127	Poly I:C adjuvanted TMC-PA nanoparticles	1088:1127	Poly I:C adjuvanted TMC-PA nanoparticles	1088:1127	Irrespective of the route of immunization, CpG or Poly I:C adjuvanted TMC-PA nanoparticles induced a significantly higher humoral response (total serum IgG and its isotypes viz., IgG1, IgG2a, and IgG2b), compared to their CpG or Poly I:C PA counterparts.
29616046	7	124	theme	adjuvant	1392:1399	arg1	potential					1401:1409	The adjuvant potential	1388:1409	The adjuvant potential of TMC nanoparticles	1388:1430	The adjuvant potential of TMC nanoparticles could be observed in all the three routes as the TMC-PA nanoparticles by themselves induced IgG titers (1-1.5 × 105) significantly higher than both CpG PA and Poly I:C PA groups (2-8 × 104).
29616046	2	125	theme	causative	447:455	arg1	anthracis					432:440	Bacillus anthracis	423:440	Bacillus anthracis	423:440	Though they can provide a protective immunity, their multiple side-effects owing to their ill-defined composition and presence of toxic proteins (LF and EF) of Bacillus anthracis, the causative organism of anthrax, in the vaccine formulation makes their widespread use objectionable.
29616046	2	125	theme	causative	447:455	arg1	organism					457:464	the causative organism	443:464	the causative organism of anthrax	443:475	Though they can provide a protective immunity, their multiple side-effects owing to their ill-defined composition and presence of toxic proteins (LF and EF) of Bacillus anthracis, the causative organism of anthrax, in the vaccine formulation makes their widespread use objectionable.
29616046	2	126	from	presence	381:388	arg1	formulation					493:503	the vaccine formulation	481:503	the vaccine formulation	481:503	Though they can provide a protective immunity, their multiple side-effects owing to their ill-defined composition and presence of toxic proteins (LF and EF) of Bacillus anthracis, the causative organism of anthrax, in the vaccine formulation makes their widespread use objectionable.
29616046	6	127	theme	I	1360:1360	arg1	behavior					1335:1342	the synergistic behavior	1319:1342	the synergistic behavior of CpG and Poly I:C with TMC nanoparticles	1319:1385	This clearly demonstrates the synergistic behavior of CpG and Poly I:C with TMC nanoparticles.
29616046	5	128	theme	Poly	1088:1091	arg1	nanoparticles					1115:1127	Poly I:C adjuvanted TMC-PA nanoparticles	1088:1127	Poly I:C adjuvanted TMC-PA nanoparticles	1088:1127	Irrespective of the route of immunization, CpG or Poly I:C adjuvanted TMC-PA nanoparticles induced a significantly higher humoral response (total serum IgG and its isotypes viz., IgG1, IgG2a, and IgG2b), compared to their CpG or Poly I:C PA counterparts.
29616046	4	129	theme	intraperitoneal	999:1013	arg1	routes					942:947	three different routes	926:947	three different routes	926:947	In this context, we have evaluated the potential of various vaccine formulations comprising of protective antigen (PA) encapsulated trimethyl-chitosan nanoparticles (TMC-PA) in conjunction with either CpG-C ODN 2395 (CpG) or Poly I:C. Each formulation was administered via three different routes, viz., subcutaneous (SC), intramuscular (IM), and intraperitoneal in female BALB/c mice.
29616046	9	130	theme	CpG	1847:1849	arg1	I					1860:1860	CpG and Poly I	1847:1860	I	1860:1860	Adjuvanation with CpG and Poly I:C, the TMC-PA nanoparticles triggered a Th1 skewed immune response, as suggested by an increase in the levels of total IgG2a along with IFN-γ cytokine production.
29616046	9	130	theme	CpG	1847:1849	arg1	nanoparticles					1876:1888	the TMC-PA nanoparticles	1865:1888	the TMC-PA nanoparticles	1865:1888	Adjuvanation with CpG and Poly I:C, the TMC-PA nanoparticles triggered a Th1 skewed immune response, as suggested by an increase in the levels of total IgG2a along with IFN-γ cytokine production.
29616046	2	131	theme	multiple	316:323	arg1	side-effects					325:336	their multiple side-effects	310:336	their multiple side-effects owing to their ill-defined composition and presence of toxic proteins (LF and EF) of Bacillus anthracis, the causative organism of anthrax, in the vaccine formulation	310:503	Though they can provide a protective immunity, their multiple side-effects owing to their ill-defined composition and presence of toxic proteins (LF and EF) of Bacillus anthracis, the causative organism of anthrax, in the vaccine formulation makes their widespread use objectionable.
29616046	5	132	theme	higher	1153:1158	arg1	response					1168:1175	a significantly higher humoral response	1137:1175	a significantly higher humoral response (total serum IgG and its isotypes viz., IgG1, IgG2a, and IgG2b)	1137:1239	Irrespective of the route of immunization, CpG or Poly I:C adjuvanted TMC-PA nanoparticles induced a significantly higher humoral response (total serum IgG and its isotypes viz., IgG1, IgG2a, and IgG2b), compared to their CpG or Poly I:C PA counterparts.
29616046	4	133	theme	female	1018:1023	arg1	mice					1032:1035	female BALB/c mice	1018:1035	female BALB/c mice	1018:1035	In this context, we have evaluated the potential of various vaccine formulations comprising of protective antigen (PA) encapsulated trimethyl-chitosan nanoparticles (TMC-PA) in conjunction with either CpG-C ODN 2395 (CpG) or Poly I:C. Each formulation was administered via three different routes, viz., subcutaneous (SC), intramuscular (IM), and intraperitoneal in female BALB/c mice.
29616046	9	134	theme	Poly	1855:1858	arg1	I					1860:1860	CpG and Poly I	1847:1860	I	1860:1860	Adjuvanation with CpG and Poly I:C, the TMC-PA nanoparticles triggered a Th1 skewed immune response, as suggested by an increase in the levels of total IgG2a along with IFN-γ cytokine production.
29616046	9	134	theme	Poly	1855:1858	arg1	nanoparticles					1876:1888	the TMC-PA nanoparticles	1865:1888	the TMC-PA nanoparticles	1865:1888	Adjuvanation with CpG and Poly I:C, the TMC-PA nanoparticles triggered a Th1 skewed immune response, as suggested by an increase in the levels of total IgG2a along with IFN-γ cytokine production.
29616046	4	135	theme	trimethyl-chitosan	785:802	arg1	TMC-PA					819:824	TMC-PA	819:824	TMC-PA	819:824	In this context, we have evaluated the potential of various vaccine formulations comprising of protective antigen (PA) encapsulated trimethyl-chitosan nanoparticles (TMC-PA) in conjunction with either CpG-C ODN 2395 (CpG) or Poly I:C. Each formulation was administered via three different routes, viz., subcutaneous (SC), intramuscular (IM), and intraperitoneal in female BALB/c mice.
29616046	4	135	theme	trimethyl-chitosan	785:802	arg1	nanoparticles					804:816	protective antigen (PA) encapsulated trimethyl-chitosan nanoparticles	748:816	protective antigen (PA) encapsulated trimethyl-chitosan nanoparticles (TMC-PA)	748:825	In this context, we have evaluated the potential of various vaccine formulations comprising of protective antigen (PA) encapsulated trimethyl-chitosan nanoparticles (TMC-PA) in conjunction with either CpG-C ODN 2395 (CpG) or Poly I:C. Each formulation was administered via three different routes, viz., subcutaneous (SC), intramuscular (IM), and intraperitoneal in female BALB/c mice.
29616046	12	136	dep	effective	2389:2397	arg1	non-toxic					2400:2408	non-toxic	2400:2408	non-toxic	2400:2408	Altogether, the results suggest that CpG or Poly I:C adjuvanted, PA-loaded TMC nanoparticles could be used as an effective, non-toxic, second generation subunit-vaccine candidate against anthrax.
29616046	12	136	dep	effective	2389:2397	arg1	second					2411:2416	second	2411:2416	second	2411:2416	Altogether, the results suggest that CpG or Poly I:C adjuvanted, PA-loaded TMC nanoparticles could be used as an effective, non-toxic, second generation subunit-vaccine candidate against anthrax.
30513319	4	0	theme	in vitro	604:611	arg1	effect					632:637	their in vitro release-modulating effect	598:637	their in vitro release-modulating effect	598:637	The particle and fiber diameter, thermal stability, material compatibility of the formed coatings, their in vitro release-modulating effect, and ocular tolerability were investigated.
30513319	8	1	dep	diffusion	1139:1147	arg1	w/v					1153:1155	1% w/v	1150:1155	1% w/v; n = 0.5	1150:1164	Kinetic modeling revealed a noticeable effect of chitosan; the higher the concentration, the more the release of TM because of chitosan swelling, with the mechanism changing from Fickian diffusion (1% w/v; n = 0.5) to non-Fickian (5% w/v, 0.45 <n < 0.89).
30513319	8	2	theme	chitosan	1079:1086	arg1	swelling					1088:1095	chitosan swelling	1079:1095	chitosan swelling	1079:1095	Kinetic modeling revealed a noticeable effect of chitosan; the higher the concentration, the more the release of TM because of chitosan swelling, with the mechanism changing from Fickian diffusion (1% w/v; n = 0.5) to non-Fickian (5% w/v, 0.45 <n < 0.89).
30513319	8	3	dep	effect	991:996	arg1	higher					1015:1020	higher	1015:1020	higher	1015:1020	Kinetic modeling revealed a noticeable effect of chitosan; the higher the concentration, the more the release of TM because of chitosan swelling, with the mechanism changing from Fickian diffusion (1% w/v; n = 0.5) to non-Fickian (5% w/v, 0.45 <n < 0.89).
30513319	1	4	theme	ocular	183:188	arg1	lenses					198:203	ocular contact lenses	183:203	ocular contact lenses	183:203	This article reports on electrohydrodynamic atomization to engineer on-demand novel coatings for ocular contact lenses.
30513319	8	5	theme	5	1183:1183	arg1	%					1184:1184	%	1184:1184	%	1184:1184	Kinetic modeling revealed a noticeable effect of chitosan; the higher the concentration, the more the release of TM because of chitosan swelling, with the mechanism changing from Fickian diffusion (1% w/v; n = 0.5) to non-Fickian (5% w/v, 0.45 <n < 0.89).
30513319	4	6	theme	thermal	532:538	arg1	stability					540:548	thermal stability	532:548	thermal stability	532:548	The particle and fiber diameter, thermal stability, material compatibility of the formed coatings, their in vitro release-modulating effect, and ocular tolerability were investigated.
30513319	5	7	theme	stable	711:716	arg1	nanomatrices					718:729	highly stable nanomatrices	704:729	highly stable nanomatrices	704:729	Results demonstrated highly stable nanomatrices with advantageous morphology and size.
30513319	4	8	theme	particle	503:510	arg1	diameter					522:529	The particle and fiber diameter	499:529	diameter	522:529	The particle and fiber diameter, thermal stability, material compatibility of the formed coatings, their in vitro release-modulating effect, and ocular tolerability were investigated.
30513319	4	9	theme	release-modulating	613:630	arg1	effect					632:637	their in vitro release-modulating effect	598:637	their in vitro release-modulating effect	598:637	The particle and fiber diameter, thermal stability, material compatibility of the formed coatings, their in vitro release-modulating effect, and ocular tolerability were investigated.
30513319	9	10	theme	ocular	1297:1302	arg1	remit					1313:1317	the ocular research remit	1293:1317	the ocular research remit	1293:1317	The use of electrohydrodynamic atomization has not yet been explored in depth within the ocular research remit, engineering on-demand lens coatings capable of sustaining TM release.
30513319	8	11	dep	non-Fickian	1170:1180	arg1	<n					1196:1197	0.45 <n < 0.89	1191:1204	0.45 <n < 0.89	1191:1204	Kinetic modeling revealed a noticeable effect of chitosan; the higher the concentration, the more the release of TM because of chitosan swelling, with the mechanism changing from Fickian diffusion (1% w/v; n = 0.5) to non-Fickian (5% w/v, 0.45 <n < 0.89).
30513319	8	11	dep	non-Fickian	1170:1180	arg1	w/v					1186:1188	5% w/v	1183:1188	5% w/v	1183:1188	Kinetic modeling revealed a noticeable effect of chitosan; the higher the concentration, the more the release of TM because of chitosan swelling, with the mechanism changing from Fickian diffusion (1% w/v; n = 0.5) to non-Fickian (5% w/v, 0.45 <n < 0.89).
30513319	6	12	theme	ocular	852:857	arg1	biocompatibility					859:874	excellent ocular biocompatibility	842:874	excellent ocular biocompatibility	842:874	All formulations yielded coatings with high TM encapsulation (>88%) and excellent ocular biocompatibility.
30513319	10	13	theme	dosage	1429:1434	arg1	form					1436:1439	an alternative dosage form	1414:1439	an alternative dosage form for management of glaucoma with particular emphasis on improving poor patient compliance	1414:1528	This is likely to offer an alternative dosage form for management of glaucoma with particular emphasis on improving poor patient compliance.
30513319	1	14	theme	contact	190:196	arg1	lenses					198:203	ocular contact lenses	183:203	ocular contact lenses	183:203	This article reports on electrohydrodynamic atomization to engineer on-demand novel coatings for ocular contact lenses.
30513319	3	15	dep	Polymers	317:324	arg1	poly					351:354	poly	351:354	poly (N-isopropylacrylamide)	351:378	Polymers polyvinylpyrrolidone and poly (N-isopropylacrylamide) were utilized to encapsulate TM and were electrically atomized to produce optimized, stationary contact lens coatings.
30513319	3	15	dep	Polymers	317:324	arg1	polyvinylpyrrolidone					326:345	polyvinylpyrrolidone	326:345	polyvinylpyrrolidone	326:345	Polymers polyvinylpyrrolidone and poly (N-isopropylacrylamide) were utilized to encapsulate TM and were electrically atomized to produce optimized, stationary contact lens coatings.
30513319	3	15	dep	Polymers	317:324	arg1	N-isopropylacrylamide					357:377	N-isopropylacrylamide	357:377	N-isopropylacrylamide	357:377	Polymers polyvinylpyrrolidone and poly (N-isopropylacrylamide) were utilized to encapsulate TM and were electrically atomized to produce optimized, stationary contact lens coatings.
30513319	3	15	dep	Polymers	317:324	arg1	Polymers					317:324	Polymers polyvinylpyrrolidone and poly (N-isopropylacrylamide)	317:378	Polymers polyvinylpyrrolidone and poly (N-isopropylacrylamide)	317:378	Polymers polyvinylpyrrolidone and poly (N-isopropylacrylamide) were utilized to encapsulate TM and were electrically atomized to produce optimized, stationary contact lens coatings.
30513319	4	16	theme	fiber	516:520	arg1	diameter					522:529	The particle and fiber diameter	499:529	diameter	522:529	The particle and fiber diameter, thermal stability, material compatibility of the formed coatings, their in vitro release-modulating effect, and ocular tolerability were investigated.
30513319	6	17	theme	excellent	842:850	arg1	biocompatibility					859:874	excellent ocular biocompatibility	842:874	excellent ocular biocompatibility	842:874	All formulations yielded coatings with high TM encapsulation (>88%) and excellent ocular biocompatibility.
30513319	9	18	theme	lens	1342:1345	arg1	coatings					1347:1354	on-demand lens coatings	1332:1354	on-demand lens coatings capable of sustaining TM release	1332:1387	The use of electrohydrodynamic atomization has not yet been explored in depth within the ocular research remit, engineering on-demand lens coatings capable of sustaining TM release.
30513319	3	19	used	utilized	385:392	arg2	Polymers					317:324	Polymers polyvinylpyrrolidone and poly (N-isopropylacrylamide)	317:378	Polymers polyvinylpyrrolidone and poly (N-isopropylacrylamide)	317:378	Polymers polyvinylpyrrolidone and poly (N-isopropylacrylamide) were utilized to encapsulate TM and were electrically atomized to produce optimized, stationary contact lens coatings.
30513319	3	19	used	utilized	385:392	arg2	polyvinylpyrrolidone					326:345	polyvinylpyrrolidone	326:345	polyvinylpyrrolidone	326:345	Polymers polyvinylpyrrolidone and poly (N-isopropylacrylamide) were utilized to encapsulate TM and were electrically atomized to produce optimized, stationary contact lens coatings.
30513319	3	19	used	utilized	385:392	arg2	poly					351:354	poly	351:354	poly (N-isopropylacrylamide)	351:378	Polymers polyvinylpyrrolidone and poly (N-isopropylacrylamide) were utilized to encapsulate TM and were electrically atomized to produce optimized, stationary contact lens coatings.
30513319	8	20	theme	noticeable	980:989	arg1	release					1054:1060	the release	1050:1060	the more the release of TM	1041:1066	Kinetic modeling revealed a noticeable effect of chitosan; the higher the concentration, the more the release of TM because of chitosan swelling, with the mechanism changing from Fickian diffusion (1% w/v; n = 0.5) to non-Fickian (5% w/v, 0.45 <n < 0.89).
30513319	8	20	theme	noticeable	980:989	arg1	effect					991:996	a noticeable effect	978:996	a noticeable effect of chitosan	978:1008	Kinetic modeling revealed a noticeable effect of chitosan; the higher the concentration, the more the release of TM because of chitosan swelling, with the mechanism changing from Fickian diffusion (1% w/v; n = 0.5) to non-Fickian (5% w/v, 0.45 <n < 0.89).
30513319	8	21	theme	%	1151:1151	arg1	w/v					1153:1155	1% w/v	1150:1155	1% w/v; n = 0.5	1150:1164	Kinetic modeling revealed a noticeable effect of chitosan; the higher the concentration, the more the release of TM because of chitosan swelling, with the mechanism changing from Fickian diffusion (1% w/v; n = 0.5) to non-Fickian (5% w/v, 0.45 <n < 0.89).
30513319	10	22	theme	particular	1473:1482	arg1	emphasis					1484:1491	particular emphasis	1473:1491	particular emphasis on improving poor patient compliance	1473:1528	This is likely to offer an alternative dosage form for management of glaucoma with particular emphasis on improving poor patient compliance.
30513319	4	23	theme	formed	581:586	arg1	coatings					588:595	the formed coatings	577:595	the formed coatings	577:595	The particle and fiber diameter, thermal stability, material compatibility of the formed coatings, their in vitro release-modulating effect, and ocular tolerability were investigated.
30513319	10	24	theme	glaucoma	1459:1466	arg1	management					1445:1454	management	1445:1454	management of glaucoma with particular emphasis on improving poor patient compliance	1445:1528	This is likely to offer an alternative dosage form for management of glaucoma with particular emphasis on improving poor patient compliance.
30513319	10	25	with	management	1445:1454	arg1	emphasis					1484:1491	particular emphasis	1473:1491	particular emphasis on improving poor patient compliance	1473:1528	This is likely to offer an alternative dosage form for management of glaucoma with particular emphasis on improving poor patient compliance.
30513319	3	26	theme	optimized	454:462	arg1	coatings					489:496	optimized, stationary contact lens coatings	454:496	optimized, stationary contact lens coatings	454:496	Polymers polyvinylpyrrolidone and poly (N-isopropylacrylamide) were utilized to encapsulate TM and were electrically atomized to produce optimized, stationary contact lens coatings.
30513319	8	27	dep	w/v	1153:1155	arg1	n =					1158:1160	n = 0.5	1158:1164	1% w/v; n = 0.5	1150:1164	Kinetic modeling revealed a noticeable effect of chitosan; the higher the concentration, the more the release of TM because of chitosan swelling, with the mechanism changing from Fickian diffusion (1% w/v; n = 0.5) to non-Fickian (5% w/v, 0.45 <n < 0.89).
30513319	8	28	theme	%	1184:1184	arg1	<n					1196:1197	0.45 <n < 0.89	1191:1204	0.45 <n < 0.89	1191:1204	Kinetic modeling revealed a noticeable effect of chitosan; the higher the concentration, the more the release of TM because of chitosan swelling, with the mechanism changing from Fickian diffusion (1% w/v; n = 0.5) to non-Fickian (5% w/v, 0.45 <n < 0.89).
30513319	8	28	theme	%	1184:1184	arg1	w/v					1186:1188	5% w/v	1183:1188	5% w/v	1183:1188	Kinetic modeling revealed a noticeable effect of chitosan; the higher the concentration, the more the release of TM because of chitosan swelling, with the mechanism changing from Fickian diffusion (1% w/v; n = 0.5) to non-Fickian (5% w/v, 0.45 <n < 0.89).
30513319	7	29	theme	triphasic	907:915	arg1	release					917:923	biphasic and triphasic release	894:923	biphasic and triphasic release	894:923	Coatings yielded biphasic and triphasic release, depending on composition.
30513319	9	30	theme	on-demand	1332:1340	arg1	coatings					1347:1354	on-demand lens coatings	1332:1354	on-demand lens coatings capable of sustaining TM release	1332:1387	The use of electrohydrodynamic atomization has not yet been explored in depth within the ocular research remit, engineering on-demand lens coatings capable of sustaining TM release.
30513319	8	31	theme	Fickian	1131:1137	arg1	diffusion					1139:1147	Fickian diffusion	1131:1147	Fickian diffusion (1% w/v; n = 0.5)	1131:1165	Kinetic modeling revealed a noticeable effect of chitosan; the higher the concentration, the more the release of TM because of chitosan swelling, with the mechanism changing from Fickian diffusion (1% w/v; n = 0.5) to non-Fickian (5% w/v, 0.45 <n < 0.89).
30513319	1	32	theme	electrohydrodynamic	110:128	arg1	atomization					130:140	electrohydrodynamic atomization	110:140	electrohydrodynamic atomization	110:140	This article reports on electrohydrodynamic atomization to engineer on-demand novel coatings for ocular contact lenses.
30513319	8	33	theme	Kinetic	952:958	arg1	modeling					960:967	Kinetic modeling	952:967	Kinetic modeling	952:967	Kinetic modeling revealed a noticeable effect of chitosan; the higher the concentration, the more the release of TM because of chitosan swelling, with the mechanism changing from Fickian diffusion (1% w/v; n = 0.5) to non-Fickian (5% w/v, 0.45 <n < 0.89).
30513319	0	34	theme	Nanocoatings	46:57	arg1	Development					16:26	Development	16:26	Development	16:26	Engineering and Development of Chitosan-Based Nanocoatings for Ocular Contact Lenses.
30513319	0	34	theme	Nanocoatings	46:57	arg1	Engineering					0:10	Engineering	0:10	Engineering	0:10	Engineering and Development of Chitosan-Based Nanocoatings for Ocular Contact Lenses.
30513319	4	35	theme	coatings	588:595	arg1	compatibility					560:572	material compatibility	551:572	material compatibility of the formed coatings	551:595	The particle and fiber diameter, thermal stability, material compatibility of the formed coatings, their in vitro release-modulating effect, and ocular tolerability were investigated.
30513319	4	35	theme	coatings	588:595	arg1	effect					632:637	their in vitro release-modulating effect	598:637	their in vitro release-modulating effect	598:637	The particle and fiber diameter, thermal stability, material compatibility of the formed coatings, their in vitro release-modulating effect, and ocular tolerability were investigated.
30513319	4	35	theme	coatings	588:595	arg1	stability					540:548	thermal stability	532:548	thermal stability	532:548	The particle and fiber diameter, thermal stability, material compatibility of the formed coatings, their in vitro release-modulating effect, and ocular tolerability were investigated.
30513319	4	35	theme	coatings	588:595	arg1	diameter					522:529	The particle and fiber diameter	499:529	diameter	522:529	The particle and fiber diameter, thermal stability, material compatibility of the formed coatings, their in vitro release-modulating effect, and ocular tolerability were investigated.
30513319	4	35	theme	coatings	588:595	arg1	tolerability					651:662	ocular tolerability	644:662	ocular tolerability	644:662	The particle and fiber diameter, thermal stability, material compatibility of the formed coatings, their in vitro release-modulating effect, and ocular tolerability were investigated.
30513319	8	36	theme	1	1150:1150	arg1	%					1151:1151	%	1151:1151	%	1151:1151	Kinetic modeling revealed a noticeable effect of chitosan; the higher the concentration, the more the release of TM because of chitosan swelling, with the mechanism changing from Fickian diffusion (1% w/v; n = 0.5) to non-Fickian (5% w/v, 0.45 <n < 0.89).
30513319	7	37	theme	biphasic	894:901	arg1	release					917:923	biphasic and triphasic release	894:923	biphasic and triphasic release	894:923	Coatings yielded biphasic and triphasic release, depending on composition.
30513319	3	38	theme	contact	476:482	arg1	coatings					489:496	optimized, stationary contact lens coatings	454:496	optimized, stationary contact lens coatings	454:496	Polymers polyvinylpyrrolidone and poly (N-isopropylacrylamide) were utilized to encapsulate TM and were electrically atomized to produce optimized, stationary contact lens coatings.
30513319	0	39	theme	Chitosan-Based	31:44	arg1	Nanocoatings					46:57	Chitosan-Based Nanocoatings	31:57	Chitosan-Based Nanocoatings	31:57	Engineering and Development of Chitosan-Based Nanocoatings for Ocular Contact Lenses.
30513319	4	40	theme	material	551:558	arg1	compatibility					560:572	material compatibility	551:572	material compatibility of the formed coatings	551:595	The particle and fiber diameter, thermal stability, material compatibility of the formed coatings, their in vitro release-modulating effect, and ocular tolerability were investigated.
30513319	9	41	theme	electrohydrodynamic	1219:1237	arg1	atomization					1239:1249	electrohydrodynamic atomization	1219:1249	electrohydrodynamic atomization	1219:1249	The use of electrohydrodynamic atomization has not yet been explored in depth within the ocular research remit, engineering on-demand lens coatings capable of sustaining TM release.
30513319	3	42	dep	optimized	454:462	arg1	stationary					465:474	stationary	465:474	stationary	465:474	Polymers polyvinylpyrrolidone and poly (N-isopropylacrylamide) were utilized to encapsulate TM and were electrically atomized to produce optimized, stationary contact lens coatings.
30513319	8	43	theme	chitosan	1001:1008	arg1	release					1054:1060	the release	1050:1060	the more the release of TM	1041:1066	Kinetic modeling revealed a noticeable effect of chitosan; the higher the concentration, the more the release of TM because of chitosan swelling, with the mechanism changing from Fickian diffusion (1% w/v; n = 0.5) to non-Fickian (5% w/v, 0.45 <n < 0.89).
30513319	8	43	theme	chitosan	1001:1008	arg1	effect					991:996	a noticeable effect	978:996	a noticeable effect of chitosan	978:1008	Kinetic modeling revealed a noticeable effect of chitosan; the higher the concentration, the more the release of TM because of chitosan swelling, with the mechanism changing from Fickian diffusion (1% w/v; n = 0.5) to non-Fickian (5% w/v, 0.45 <n < 0.89).
30513319	3	44	theme	lens	484:487	arg1	coatings					489:496	optimized, stationary contact lens coatings	454:496	optimized, stationary contact lens coatings	454:496	Polymers polyvinylpyrrolidone and poly (N-isopropylacrylamide) were utilized to encapsulate TM and were electrically atomized to produce optimized, stationary contact lens coatings.
30513319	0	45	theme	Ocular	63:68	arg1	Lenses					78:83	Ocular Contact Lenses	63:83	Ocular Contact Lenses	63:83	Engineering and Development of Chitosan-Based Nanocoatings for Ocular Contact Lenses.
30513319	9	46	theme	capable	1356:1362	arg1	coatings					1347:1354	on-demand lens coatings	1332:1354	on-demand lens coatings capable of sustaining TM release	1332:1387	The use of electrohydrodynamic atomization has not yet been explored in depth within the ocular research remit, engineering on-demand lens coatings capable of sustaining TM release.
30513319	9	47	theme	atomization	1239:1249	arg1	use					1212:1214	The use	1208:1214	The use of electrohydrodynamic atomization	1208:1249	The use of electrohydrodynamic atomization has not yet been explored in depth within the ocular research remit, engineering on-demand lens coatings capable of sustaining TM release.
30513319	9	48	theme	research	1304:1311	arg1	remit					1313:1317	the ocular research remit	1293:1317	the ocular research remit	1293:1317	The use of electrohydrodynamic atomization has not yet been explored in depth within the ocular research remit, engineering on-demand lens coatings capable of sustaining TM release.
30513319	10	49	theme	patient	1511:1517	arg1	compliance					1519:1528	poor patient compliance	1506:1528	poor patient compliance	1506:1528	This is likely to offer an alternative dosage form for management of glaucoma with particular emphasis on improving poor patient compliance.
30513319	5	50	theme	advantageous	736:747	arg1	morphology					749:758	advantageous morphology	736:758	advantageous morphology	736:758	Results demonstrated highly stable nanomatrices with advantageous morphology and size.
30513319	10	51	theme	alternative	1417:1427	arg1	form					1436:1439	an alternative dosage form	1414:1439	an alternative dosage form for management of glaucoma with particular emphasis on improving poor patient compliance	1414:1528	This is likely to offer an alternative dosage form for management of glaucoma with particular emphasis on improving poor patient compliance.
30513319	10	52	theme	poor	1506:1509	arg1	compliance					1519:1528	poor patient compliance	1506:1528	poor patient compliance	1506:1528	This is likely to offer an alternative dosage form for management of glaucoma with particular emphasis on improving poor patient compliance.
30513319	8	53	theme	TM	1065:1066	arg1	release					1054:1060	the release	1050:1060	the more the release of TM	1041:1066	Kinetic modeling revealed a noticeable effect of chitosan; the higher the concentration, the more the release of TM because of chitosan swelling, with the mechanism changing from Fickian diffusion (1% w/v; n = 0.5) to non-Fickian (5% w/v, 0.45 <n < 0.89).
30513319	8	53	theme	TM	1065:1066	arg1	effect					991:996	a noticeable effect	978:996	a noticeable effect of chitosan	978:1008	Kinetic modeling revealed a noticeable effect of chitosan; the higher the concentration, the more the release of TM because of chitosan swelling, with the mechanism changing from Fickian diffusion (1% w/v; n = 0.5) to non-Fickian (5% w/v, 0.45 <n < 0.89).
30513319	6	54	theme	TM	814:815	arg1	encapsulation					817:829	high TM encapsulation	809:829	high TM encapsulation (>88%)	809:836	All formulations yielded coatings with high TM encapsulation (>88%) and excellent ocular biocompatibility.
30513319	6	54	theme	TM	814:815	arg1	%					835:835	>88%	832:835	>88%	832:835	All formulations yielded coatings with high TM encapsulation (>88%) and excellent ocular biocompatibility.
30513319	9	55	theme	TM	1378:1379	arg1	release					1381:1387	TM release	1378:1387	TM release	1378:1387	The use of electrohydrodynamic atomization has not yet been explored in depth within the ocular research remit, engineering on-demand lens coatings capable of sustaining TM release.
30513319	2	56	theme	maleate	276:282	arg1	release					257:263	the release	253:263	the release of timolol maleate (TM) using chitosan and borneol	253:314	A formulation approach was adopted to modulate the release of timolol maleate (TM) using chitosan and borneol.
30513319	6	57	theme	high	809:812	arg1	encapsulation					817:829	high TM encapsulation	809:829	high TM encapsulation (>88%)	809:836	All formulations yielded coatings with high TM encapsulation (>88%) and excellent ocular biocompatibility.
30513319	6	57	theme	high	809:812	arg1	%					835:835	>88%	832:835	>88%	832:835	All formulations yielded coatings with high TM encapsulation (>88%) and excellent ocular biocompatibility.
30513319	1	58	theme	on-demand	154:162	arg1	coatings					170:177	on-demand novel coatings	154:177	on-demand novel coatings	154:177	This article reports on electrohydrodynamic atomization to engineer on-demand novel coatings for ocular contact lenses.
30513319	2	59	theme	formulation	208:218	arg1	approach					220:227	A formulation approach	206:227	A formulation approach	206:227	A formulation approach was adopted to modulate the release of timolol maleate (TM) using chitosan and borneol.
30513319	2	60	theme	timolol	268:274	arg1	TM					285:286	TM	285:286	TM	285:286	A formulation approach was adopted to modulate the release of timolol maleate (TM) using chitosan and borneol.
30513319	2	60	theme	timolol	268:274	arg1	maleate					276:282	timolol maleate	268:282	timolol maleate (TM) using chitosan and borneol	268:314	A formulation approach was adopted to modulate the release of timolol maleate (TM) using chitosan and borneol.
30513319	1	61	theme	novel	164:168	arg1	coatings					170:177	on-demand novel coatings	154:177	on-demand novel coatings	154:177	This article reports on electrohydrodynamic atomization to engineer on-demand novel coatings for ocular contact lenses.
30513319	8	62	dep	higher	1015:1020	arg1	concentration					1026:1038	the concentration	1022:1038	the higher the concentration	1011:1038	Kinetic modeling revealed a noticeable effect of chitosan; the higher the concentration, the more the release of TM because of chitosan swelling, with the mechanism changing from Fickian diffusion (1% w/v; n = 0.5) to non-Fickian (5% w/v, 0.45 <n < 0.89).
30513319	0	63	theme	Contact	70:76	arg1	Lenses					78:83	Ocular Contact Lenses	63:83	Ocular Contact Lenses	63:83	Engineering and Development of Chitosan-Based Nanocoatings for Ocular Contact Lenses.
30513319	4	64	theme	ocular	644:649	arg1	tolerability					651:662	ocular tolerability	644:662	ocular tolerability	644:662	The particle and fiber diameter, thermal stability, material compatibility of the formed coatings, their in vitro release-modulating effect, and ocular tolerability were investigated.
29579292	7	0	from	one	1431:1433	arg1	ones					1486:1489	the warmest ones	1474:1489	the warmest ones	1474:1489	Furthermore, an 'earliness effect' was suggested by a significant relationship between spring temperature and δ13C in the coldest sites and by a positive one between winter temperature and δ13C in the warmest ones.
29579292	4	1	theme	trees	963:967	arg1	growth					953:958	growth	953:958	growth of trees in different sites since 1950	953:997	The main objectives of this study were to assess (i) the effect of climate on the carbon signature of tree-ring cellulose (δ13C); (ii) the physiological response to recent CO2 concentration increment and to climatic variation; and (iii) the relationship between intrinsic water-use efficiency (iWUE, here the average long-term ratio of net photosynthesis to stomatal conductance) and growth of trees in different sites since 1950.
29579292	4	1	theme	trees	963:967	arg1	effect					626:631	the effect	622:631	(i) the effect of climate on the carbon signature of tree-ring cellulose (δ13C); (ii) the physiological response to recent CO2 concentration increment and to climatic variation; and (iii) the relationship between intrinsic water-use efficiency (iWUE, here the average long-term ratio of net photosynthesis to stomatal conductance)	618:947	The main objectives of this study were to assess (i) the effect of climate on the carbon signature of tree-ring cellulose (δ13C); (ii) the physiological response to recent CO2 concentration increment and to climatic variation; and (iii) the relationship between intrinsic water-use efficiency (iWUE, here the average long-term ratio of net photosynthesis to stomatal conductance) and growth of trees in different sites since 1950.
29579292	8	2	theme	maturity	1527:1534	arg1	phase					1536:1540	the maturity phase	1523:1540	the maturity phase	1523:1540	In all the study sites, during the maturity phase, a positive correlation between the increment of CO2 and iWUE was observed, due to an active response of trees to CO2 increment.
29579292	2	3	theme	economic	326:333	arg1	impacts					335:341	strong ecological and economic impacts	304:341	strong ecological and economic impacts	304:341	Beech is one of the most important forest tree species in Europe, hence possible adverse factors affecting its physiology and productivity can have strong ecological and economic impacts.
29579292	8	4	theme	CO2	1591:1593	arg1	increment					1578:1586	the increment	1574:1586	the increment of CO2 and iWUE	1574:1602	In all the study sites, during the maturity phase, a positive correlation between the increment of CO2 and iWUE was observed, due to an active response of trees to CO2 increment.
29579292	8	5	located	observed	1608:1615	arg1	sites					1509:1513	all the study sites	1495:1513	all the study sites	1495:1513	In all the study sites, during the maturity phase, a positive correlation between the increment of CO2 and iWUE was observed, due to an active response of trees to CO2 increment.
29579292	8	5	located	observed	1608:1615	arg2	correlation					1554:1564	a positive correlation	1543:1564	a positive correlation between the increment of CO2 and iWUE	1543:1602	In all the study sites, during the maturity phase, a positive correlation between the increment of CO2 and iWUE was observed, due to an active response of trees to CO2 increment.
29579292	6	6	theme	temperature	1145:1155	arg1	control					1109:1115	a climatic control	1098:1115	a climatic control of summer precipitation and temperature on stomatal conductance	1098:1179	In northern sites, a climatic control of summer precipitation and temperature on stomatal conductance was demonstrated by their opposite correlations with δ13C, negative and positive, respectively.
29579292	7	7	theme	spring	1364:1369	arg1	temperature					1371:1381	spring temperature	1364:1381	spring temperature	1364:1381	Furthermore, an 'earliness effect' was suggested by a significant relationship between spring temperature and δ13C in the coldest sites and by a positive one between winter temperature and δ13C in the warmest ones.
29579292	4	8	theme	different	972:980	arg1	sites					982:986	different sites	972:986	different sites since 1950	972:997	The main objectives of this study were to assess (i) the effect of climate on the carbon signature of tree-ring cellulose (δ13C); (ii) the physiological response to recent CO2 concentration increment and to climatic variation; and (iii) the relationship between intrinsic water-use efficiency (iWUE, here the average long-term ratio of net photosynthesis to stomatal conductance) and growth of trees in different sites since 1950.
29579292	8	9	theme	iWUE	1599:1602	arg1	increment					1578:1586	the increment	1574:1586	the increment of CO2 and iWUE	1574:1602	In all the study sites, during the maturity phase, a positive correlation between the increment of CO2 and iWUE was observed, due to an active response of trees to CO2 increment.
29579292	4	10	theme	climatic	776:783	arg1	variation					785:793	climatic variation	776:793	climatic variation	776:793	The main objectives of this study were to assess (i) the effect of climate on the carbon signature of tree-ring cellulose (δ13C); (ii) the physiological response to recent CO2 concentration increment and to climatic variation; and (iii) the relationship between intrinsic water-use efficiency (iWUE, here the average long-term ratio of net photosynthesis to stomatal conductance) and growth of trees in different sites since 1950.
29579292	9	11	theme	trend	1741:1745	arg1	increment					1676:1684	This increment	1671:1684	This increment of CO2	1671:1691	This increment of CO2 was the main driver of the long term increasing trend of iWUE, resulting by an active response of trees to CO2 fertilization.
29579292	9	11	theme	trend	1741:1745	arg1	driver					1706:1711	the main driver	1697:1711	the main driver of the long term increasing trend of iWUE	1697:1753	This increment of CO2 was the main driver of the long term increasing trend of iWUE, resulting by an active response of trees to CO2 fertilization.
29579292	2	12	theme	adverse	237:243	arg1	factors					245:251	possible adverse factors	228:251	possible adverse factors affecting its physiology and productivity	228:293	Beech is one of the most important forest tree species in Europe, hence possible adverse factors affecting its physiology and productivity can have strong ecological and economic impacts.
29579292	6	13	theme	northern	1082:1089	arg1	sites					1091:1095	northern sites	1082:1095	northern sites	1082:1095	In northern sites, a climatic control of summer precipitation and temperature on stomatal conductance was demonstrated by their opposite correlations with δ13C, negative and positive, respectively.
29579292	4	14	theme	iWUE	863:866	arg1	signature					658:666	the carbon signature	647:666	the carbon signature of tree-ring cellulose (δ13C); (ii) the physiological response to recent CO2 concentration increment and to climatic variation; and (iii) the relationship between intrinsic water-use efficiency (iWUE, here the average long-term ratio of net photosynthesis to stomatal conductance	647:946	The main objectives of this study were to assess (i) the effect of climate on the carbon signature of tree-ring cellulose (δ13C); (ii) the physiological response to recent CO2 concentration increment and to climatic variation; and (iii) the relationship between intrinsic water-use efficiency (iWUE, here the average long-term ratio of net photosynthesis to stomatal conductance) and growth of trees in different sites since 1950.
29579292	6	15	theme	stomatal	1160:1167	arg1	conductance					1169:1179	stomatal conductance	1160:1179	stomatal conductance	1160:1179	In northern sites, a climatic control of summer precipitation and temperature on stomatal conductance was demonstrated by their opposite correlations with δ13C, negative and positive, respectively.
29579292	9	16	theme	trees	1791:1795	arg1	response					1779:1786	an active response	1769:1786	an active response of trees to CO2 fertilization	1769:1816	This increment of CO2 was the main driver of the long term increasing trend of iWUE, resulting by an active response of trees to CO2 fertilization.
29579292	2	17	theme	species	203:209	arg1	species					203:209	the most important forest tree species	172:209	the most important forest tree species in Europe	172:219	Beech is one of the most important forest tree species in Europe, hence possible adverse factors affecting its physiology and productivity can have strong ecological and economic impacts.
29579292	2	17	theme	species	203:209	arg1	one					165:167	one	165:167	one	165:167	Beech is one of the most important forest tree species in Europe, hence possible adverse factors affecting its physiology and productivity can have strong ecological and economic impacts.
29579292	13	18	theme	Increasing	2155:2164	arg1	iWUE					2166:2169	Increasing iWUE	2155:2169	Increasing iWUE	2155:2169	Increasing iWUE was correlated to growth in the two sites during the release phase and we hypothesize a positive effect of silvicultural treatments.
29579292	4	19	theme	relationship	810:821	arg1	signature					658:666	the carbon signature	647:666	the carbon signature of tree-ring cellulose (δ13C); (ii) the physiological response to recent CO2 concentration increment and to climatic variation; and (iii) the relationship between intrinsic water-use efficiency (iWUE, here the average long-term ratio of net photosynthesis to stomatal conductance	647:946	The main objectives of this study were to assess (i) the effect of climate on the carbon signature of tree-ring cellulose (δ13C); (ii) the physiological response to recent CO2 concentration increment and to climatic variation; and (iii) the relationship between intrinsic water-use efficiency (iWUE, here the average long-term ratio of net photosynthesis to stomatal conductance) and growth of trees in different sites since 1950.
29579292	7	20	theme	earliness	1294:1302	arg1	effect					1304:1309	an 'earliness effect'	1290:1310	an 'earliness effect'	1290:1310	Furthermore, an 'earliness effect' was suggested by a significant relationship between spring temperature and δ13C in the coldest sites and by a positive one between winter temperature and δ13C in the warmest ones.
29579292	9	21	theme	CO2	1800:1802	arg1	fertilization					1804:1816	CO2 fertilization	1800:1816	CO2 fertilization	1800:1816	This increment of CO2 was the main driver of the long term increasing trend of iWUE, resulting by an active response of trees to CO2 fertilization.
29579292	13	22	theme	treatments	2292:2301	arg1	effect					2268:2273	a positive effect	2257:2273	a positive effect of silvicultural treatments	2257:2301	Increasing iWUE was correlated to growth in the two sites during the release phase and we hypothesize a positive effect of silvicultural treatments.
29579292	2	23	theme	forest	191:196	arg1	species					203:209	the most important forest tree species	172:209	the most important forest tree species in Europe	172:219	Beech is one of the most important forest tree species in Europe, hence possible adverse factors affecting its physiology and productivity can have strong ecological and economic impacts.
29579292	8	24	theme	trees	1647:1651	arg1	response					1635:1642	an active response	1625:1642	an active response of trees to CO2 increment	1625:1668	In all the study sites, during the maturity phase, a positive correlation between the increment of CO2 and iWUE was observed, due to an active response of trees to CO2 increment.
29579292	4	25	theme	intrinsic	831:839	arg1	efficiency					851:860	intrinsic water-use efficiency	831:860	intrinsic water-use efficiency	831:860	The main objectives of this study were to assess (i) the effect of climate on the carbon signature of tree-ring cellulose (δ13C); (ii) the physiological response to recent CO2 concentration increment and to climatic variation; and (iii) the relationship between intrinsic water-use efficiency (iWUE, here the average long-term ratio of net photosynthesis to stomatal conductance) and growth of trees in different sites since 1950.
29579292	12	26	theme	southernmost	2135:2146	arg1	sites					2148:2152	the northernmost and southernmost sites	2114:2152	the northernmost and southernmost sites	2114:2152	Moreover, the sensitivity of iWUE to the increase of CO2 was different between the northernmost and southernmost sites.
29579292	6	27	theme	precipitation	1127:1139	arg1	control					1109:1115	a climatic control	1098:1115	a climatic control of summer precipitation and temperature on stomatal conductance	1098:1179	In northern sites, a climatic control of summer precipitation and temperature on stomatal conductance was demonstrated by their opposite correlations with δ13C, negative and positive, respectively.
29579292	4	28	theme	cellulose	681:689	arg1	signature					658:666	the carbon signature	647:666	the carbon signature of tree-ring cellulose (δ13C); (ii) the physiological response to recent CO2 concentration increment and to climatic variation; and (iii) the relationship between intrinsic water-use efficiency (iWUE, here the average long-term ratio of net photosynthesis to stomatal conductance	647:946	The main objectives of this study were to assess (i) the effect of climate on the carbon signature of tree-ring cellulose (δ13C); (ii) the physiological response to recent CO2 concentration increment and to climatic variation; and (iii) the relationship between intrinsic water-use efficiency (iWUE, here the average long-term ratio of net photosynthesis to stomatal conductance) and growth of trees in different sites since 1950.
29579292	6	29	theme	climatic	1100:1107	arg1	control					1109:1115	a climatic control	1098:1115	a climatic control of summer precipitation and temperature on stomatal conductance	1098:1179	In northern sites, a climatic control of summer precipitation and temperature on stomatal conductance was demonstrated by their opposite correlations with δ13C, negative and positive, respectively.
29579292	8	30	theme	CO2	1656:1658	arg1	increment					1660:1668	CO2 increment	1656:1668	CO2 increment	1656:1668	In all the study sites, during the maturity phase, a positive correlation between the increment of CO2 and iWUE was observed, due to an active response of trees to CO2 increment.
29579292	1	31	theme	isotope	124:130	arg1	analyses					132:139	carbon isotope analyses	117:139	carbon isotope analyses in tree rings	117:153	Evidences from carbon isotope analyses in tree rings.
29579292	4	32	theme	ratio	896:900	arg1	signature					658:666	the carbon signature	647:666	the carbon signature of tree-ring cellulose (δ13C); (ii) the physiological response to recent CO2 concentration increment and to climatic variation; and (iii) the relationship between intrinsic water-use efficiency (iWUE, here the average long-term ratio of net photosynthesis to stomatal conductance	647:946	The main objectives of this study were to assess (i) the effect of climate on the carbon signature of tree-ring cellulose (δ13C); (ii) the physiological response to recent CO2 concentration increment and to climatic variation; and (iii) the relationship between intrinsic water-use efficiency (iWUE, here the average long-term ratio of net photosynthesis to stomatal conductance) and growth of trees in different sites since 1950.
29579292	9	33	theme	iWUE	1750:1753	arg1	trend					1741:1745	the long term increasing trend	1716:1745	the long term increasing trend of iWUE	1716:1753	This increment of CO2 was the main driver of the long term increasing trend of iWUE, resulting by an active response of trees to CO2 fertilization.
29579292	3	34	from	cellulose	558:566	arg1	determinations					493:506	chronological determinations	479:506	chronological determinations of carbon isotope composition (δ13C) in tree-ring cellulose	479:566	In this context, four beech forests along a latitudinal gradient from southern Apennines to middle European lowlands were selected for chronological determinations of carbon isotope composition (δ13C) in tree-ring cellulose.
29579292	0	35	theme	forest	55:60	arg1	management					62:71	forest management	55:71	forest management	55:71	Do atmospheric CO2 concentration increase, climate and forest management affect iWUE of common beech?
29579292	5	36	theme	climatic	1035:1042	arg1	conditions					1044:1053	site climatic conditions	1030:1053	site climatic conditions	1030:1053	Our results demonstrated that site climatic conditions peculiarly affect δ13C.
29579292	7	37	from	relationship	1343:1354	arg1	sites					1407:1411	the coldest sites	1395:1411	the coldest sites	1395:1411	Furthermore, an 'earliness effect' was suggested by a significant relationship between spring temperature and δ13C in the coldest sites and by a positive one between winter temperature and δ13C in the warmest ones.
29579292	4	38	theme	net	905:907	arg1	photosynthesis					909:922	net photosynthesis	905:922	net photosynthesis	905:922	The main objectives of this study were to assess (i) the effect of climate on the carbon signature of tree-ring cellulose (δ13C); (ii) the physiological response to recent CO2 concentration increment and to climatic variation; and (iii) the relationship between intrinsic water-use efficiency (iWUE, here the average long-term ratio of net photosynthesis to stomatal conductance) and growth of trees in different sites since 1950.
29579292	4	39	theme	carbon	651:656	arg1	signature					658:666	the carbon signature	647:666	the carbon signature of tree-ring cellulose (δ13C); (ii) the physiological response to recent CO2 concentration increment and to climatic variation; and (iii) the relationship between intrinsic water-use efficiency (iWUE, here the average long-term ratio of net photosynthesis to stomatal conductance	647:946	The main objectives of this study were to assess (i) the effect of climate on the carbon signature of tree-ring cellulose (δ13C); (ii) the physiological response to recent CO2 concentration increment and to climatic variation; and (iii) the relationship between intrinsic water-use efficiency (iWUE, here the average long-term ratio of net photosynthesis to stomatal conductance) and growth of trees in different sites since 1950.
29579292	3	40	from	composition	526:536	arg1	cellulose					558:566	tree-ring cellulose	548:566	tree-ring cellulose	548:566	In this context, four beech forests along a latitudinal gradient from southern Apennines to middle European lowlands were selected for chronological determinations of carbon isotope composition (δ13C) in tree-ring cellulose.
29579292	4	41	from	trees	963:967	arg1	sites					982:986	different sites	972:986	different sites since 1950	972:997	The main objectives of this study were to assess (i) the effect of climate on the carbon signature of tree-ring cellulose (δ13C); (ii) the physiological response to recent CO2 concentration increment and to climatic variation; and (iii) the relationship between intrinsic water-use efficiency (iWUE, here the average long-term ratio of net photosynthesis to stomatal conductance) and growth of trees in different sites since 1950.
29579292	12	42	theme	northernmost	2118:2129	arg1	sites					2148:2152	the northernmost and southernmost sites	2114:2152	the northernmost and southernmost sites	2114:2152	Moreover, the sensitivity of iWUE to the increase of CO2 was different between the northernmost and southernmost sites.
29579292	10	43	theme	inter-annual	1891:1902	arg1	variations					1904:1913	the inter-annual variations	1887:1913	the inter-annual variations of iWUE of the southernmost and northernmost sites, respectively	1887:1978	Moreover, precipitation mostly influences positively and negatively the inter-annual variations of iWUE of the southernmost and northernmost sites, respectively.
29579292	1	44	from	analyses	132:139	arg1	Evidences					102:110	Evidences	102:110	Evidences	102:110	Evidences from carbon isotope analyses in tree rings.
29579292	1	44	from	analyses	132:139	arg1	rings					149:153	tree rings	144:153	tree rings	144:153	Evidences from carbon isotope analyses in tree rings.
29579292	13	45	theme	positive	2259:2266	arg1	effect					2268:2273	a positive effect	2257:2273	a positive effect of silvicultural treatments	2257:2301	Increasing iWUE was correlated to growth in the two sites during the release phase and we hypothesize a positive effect of silvicultural treatments.
29579292	4	46	theme	average	878:884	arg1	ratio					896:900	the average long-term ratio	874:900	the average long-term ratio of net photosynthesis to stomatal conductance	874:946	The main objectives of this study were to assess (i) the effect of climate on the carbon signature of tree-ring cellulose (δ13C); (ii) the physiological response to recent CO2 concentration increment and to climatic variation; and (iii) the relationship between intrinsic water-use efficiency (iWUE, here the average long-term ratio of net photosynthesis to stomatal conductance) and growth of trees in different sites since 1950.
29579292	0	47	theme	beech	95:99	arg1	iWUE					80:83	iWUE	80:83	iWUE of common beech	80:99	Do atmospheric CO2 concentration increase, climate and forest management affect iWUE of common beech?
29579292	3	48	theme	latitudinal	388:398	arg1	gradient					400:407	a latitudinal gradient	386:407	a latitudinal gradient from southern Apennines to middle European lowlands	386:459	In this context, four beech forests along a latitudinal gradient from southern Apennines to middle European lowlands were selected for chronological determinations of carbon isotope composition (δ13C) in tree-ring cellulose.
29579292	3	49	theme	middle	436:441	arg1	lowlands					452:459	middle European lowlands	436:459	middle European lowlands	436:459	In this context, four beech forests along a latitudinal gradient from southern Apennines to middle European lowlands were selected for chronological determinations of carbon isotope composition (δ13C) in tree-ring cellulose.
29579292	0	50	theme	CO2	15:17	arg1	increase					33:40	atmospheric CO2 concentration increase	3:40	atmospheric CO2 concentration increase	3:40	Do atmospheric CO2 concentration increase, climate and forest management affect iWUE of common beech?
29579292	4	51	theme	concentration	745:757	arg1	increment					759:767	recent CO2 concentration increment	734:767	recent CO2 concentration increment	734:767	The main objectives of this study were to assess (i) the effect of climate on the carbon signature of tree-ring cellulose (δ13C); (ii) the physiological response to recent CO2 concentration increment and to climatic variation; and (iii) the relationship between intrinsic water-use efficiency (iWUE, here the average long-term ratio of net photosynthesis to stomatal conductance) and growth of trees in different sites since 1950.
29579292	7	52	theme	winter	1443:1448	arg1	temperature					1450:1460	winter temperature	1443:1460	winter temperature	1443:1460	Furthermore, an 'earliness effect' was suggested by a significant relationship between spring temperature and δ13C in the coldest sites and by a positive one between winter temperature and δ13C in the warmest ones.
29579292	3	53	theme	beech	366:370	arg1	forests					372:378	four beech forests	361:378	four beech forests along a latitudinal gradient from southern Apennines to middle European lowlands	361:459	In this context, four beech forests along a latitudinal gradient from southern Apennines to middle European lowlands were selected for chronological determinations of carbon isotope composition (δ13C) in tree-ring cellulose.
29579292	2	54	contain	have	299:302	arg2	impacts					335:341	strong ecological and economic impacts	304:341	strong ecological and economic impacts	304:341	Beech is one of the most important forest tree species in Europe, hence possible adverse factors affecting its physiology and productivity can have strong ecological and economic impacts.
29579292	2	54	contain	have	299:302	arg1	factors					245:251	possible adverse factors	228:251	possible adverse factors affecting its physiology and productivity	228:293	Beech is one of the most important forest tree species in Europe, hence possible adverse factors affecting its physiology and productivity can have strong ecological and economic impacts.
29579292	13	55	theme	release	2224:2230	arg1	phase					2232:2236	the release phase	2220:2236	the release phase	2220:2236	Increasing iWUE was correlated to growth in the two sites during the release phase and we hypothesize a positive effect of silvicultural treatments.
29579292	10	56	theme	southernmost	1930:1941	arg1	sites					1960:1964	the southernmost and northernmost sites	1926:1964	the southernmost and northernmost sites	1926:1964	Moreover, precipitation mostly influences positively and negatively the inter-annual variations of iWUE of the southernmost and northernmost sites, respectively.
29579292	4	57	theme	response	722:729	arg1	signature					658:666	the carbon signature	647:666	the carbon signature of tree-ring cellulose (δ13C); (ii) the physiological response to recent CO2 concentration increment and to climatic variation; and (iii) the relationship between intrinsic water-use efficiency (iWUE, here the average long-term ratio of net photosynthesis to stomatal conductance	647:946	The main objectives of this study were to assess (i) the effect of climate on the carbon signature of tree-ring cellulose (δ13C); (ii) the physiological response to recent CO2 concentration increment and to climatic variation; and (iii) the relationship between intrinsic water-use efficiency (iWUE, here the average long-term ratio of net photosynthesis to stomatal conductance) and growth of trees in different sites since 1950.
29579292	2	58	theme	strong	304:309	arg1	impacts					335:341	strong ecological and economic impacts	304:341	strong ecological and economic impacts	304:341	Beech is one of the most important forest tree species in Europe, hence possible adverse factors affecting its physiology and productivity can have strong ecological and economic impacts.
29579292	3	59	theme	composition	526:536	arg1	determinations					493:506	chronological determinations	479:506	chronological determinations of carbon isotope composition (δ13C) in tree-ring cellulose	479:566	In this context, four beech forests along a latitudinal gradient from southern Apennines to middle European lowlands were selected for chronological determinations of carbon isotope composition (δ13C) in tree-ring cellulose.
29579292	4	60	dep	effect	626:631	arg1	i					619:619	i	619:619	i	619:619	The main objectives of this study were to assess (i) the effect of climate on the carbon signature of tree-ring cellulose (δ13C); (ii) the physiological response to recent CO2 concentration increment and to climatic variation; and (iii) the relationship between intrinsic water-use efficiency (iWUE, here the average long-term ratio of net photosynthesis to stomatal conductance) and growth of trees in different sites since 1950.
29579292	9	61	theme	long	1720:1723	arg1	trend					1741:1745	the long term increasing trend	1716:1745	the long term increasing trend of iWUE	1716:1753	This increment of CO2 was the main driver of the long term increasing trend of iWUE, resulting by an active response of trees to CO2 fertilization.
29579292	8	62	theme	study	1503:1507	arg1	sites					1509:1513	all the study sites	1495:1513	all the study sites	1495:1513	In all the study sites, during the maturity phase, a positive correlation between the increment of CO2 and iWUE was observed, due to an active response of trees to CO2 increment.
29579292	4	63	theme	recent	734:739	arg1	increment					759:767	recent CO2 concentration increment	734:767	recent CO2 concentration increment	734:767	The main objectives of this study were to assess (i) the effect of climate on the carbon signature of tree-ring cellulose (δ13C); (ii) the physiological response to recent CO2 concentration increment and to climatic variation; and (iii) the relationship between intrinsic water-use efficiency (iWUE, here the average long-term ratio of net photosynthesis to stomatal conductance) and growth of trees in different sites since 1950.
29579292	6	64	with	correlations	1216:1227	arg1	δ13C					1234:1237	δ13C	1234:1237	δ13C	1234:1237	In northern sites, a climatic control of summer precipitation and temperature on stomatal conductance was demonstrated by their opposite correlations with δ13C, negative and positive, respectively.
29579292	9	65	theme	main	1701:1704	arg1	increment					1676:1684	This increment	1671:1684	This increment of CO2	1671:1691	This increment of CO2 was the main driver of the long term increasing trend of iWUE, resulting by an active response of trees to CO2 fertilization.
29579292	9	65	theme	main	1701:1704	arg1	driver					1706:1711	the main driver	1697:1711	the main driver of the long term increasing trend of iWUE	1697:1753	This increment of CO2 was the main driver of the long term increasing trend of iWUE, resulting by an active response of trees to CO2 fertilization.
29579292	9	66	theme	increasing	1730:1739	arg1	trend					1741:1745	the long term increasing trend	1716:1745	the long term increasing trend of iWUE	1716:1753	This increment of CO2 was the main driver of the long term increasing trend of iWUE, resulting by an active response of trees to CO2 fertilization.
29579292	4	67	theme	study	597:601	arg1	objectives					578:587	The main objectives	569:587	The main objectives of this study	569:601	The main objectives of this study were to assess (i) the effect of climate on the carbon signature of tree-ring cellulose (δ13C); (ii) the physiological response to recent CO2 concentration increment and to climatic variation; and (iii) the relationship between intrinsic water-use efficiency (iWUE, here the average long-term ratio of net photosynthesis to stomatal conductance) and growth of trees in different sites since 1950.
29579292	3	68	theme	chronological	479:491	arg1	determinations					493:506	chronological determinations	479:506	chronological determinations of carbon isotope composition (δ13C) in tree-ring cellulose	479:566	In this context, four beech forests along a latitudinal gradient from southern Apennines to middle European lowlands were selected for chronological determinations of carbon isotope composition (δ13C) in tree-ring cellulose.
29579292	7	69	theme	coldest	1399:1405	arg1	sites					1407:1411	the coldest sites	1395:1411	the coldest sites	1395:1411	Furthermore, an 'earliness effect' was suggested by a significant relationship between spring temperature and δ13C in the coldest sites and by a positive one between winter temperature and δ13C in the warmest ones.
29579292	10	70	theme	northernmost	1947:1958	arg1	sites					1960:1964	the southernmost and northernmost sites	1926:1964	the southernmost and northernmost sites	1926:1964	Moreover, precipitation mostly influences positively and negatively the inter-annual variations of iWUE of the southernmost and northernmost sites, respectively.
29579292	6	71	theme	opposite	1207:1214	arg1	negative					1240:1247	negative	1240:1247	negative	1240:1247	In northern sites, a climatic control of summer precipitation and temperature on stomatal conductance was demonstrated by their opposite correlations with δ13C, negative and positive, respectively.
29579292	6	71	theme	opposite	1207:1214	arg1	correlations					1216:1227	their opposite correlations	1201:1227	their opposite correlations with δ13C	1201:1237	In northern sites, a climatic control of summer precipitation and temperature on stomatal conductance was demonstrated by their opposite correlations with δ13C, negative and positive, respectively.
29579292	6	71	theme	opposite	1207:1214	arg1	positive					1253:1260	positive	1253:1260	positive	1253:1260	In northern sites, a climatic control of summer precipitation and temperature on stomatal conductance was demonstrated by their opposite correlations with δ13C, negative and positive, respectively.
29579292	3	72	from	determinations	493:506	arg1	cellulose					558:566	tree-ring cellulose	548:566	tree-ring cellulose	548:566	In this context, four beech forests along a latitudinal gradient from southern Apennines to middle European lowlands were selected for chronological determinations of carbon isotope composition (δ13C) in tree-ring cellulose.
29579292	4	73	theme	main	573:576	arg1	objectives					578:587	The main objectives	569:587	The main objectives of this study	569:601	The main objectives of this study were to assess (i) the effect of climate on the carbon signature of tree-ring cellulose (δ13C); (ii) the physiological response to recent CO2 concentration increment and to climatic variation; and (iii) the relationship between intrinsic water-use efficiency (iWUE, here the average long-term ratio of net photosynthesis to stomatal conductance) and growth of trees in different sites since 1950.
29579292	6	74	from	control	1109:1115	arg1	conductance					1169:1179	stomatal conductance	1160:1179	stomatal conductance	1160:1179	In northern sites, a climatic control of summer precipitation and temperature on stomatal conductance was demonstrated by their opposite correlations with δ13C, negative and positive, respectively.
29579292	3	75	theme	isotope	518:524	arg1	δ13C					539:542	δ13C	539:542	δ13C	539:542	In this context, four beech forests along a latitudinal gradient from southern Apennines to middle European lowlands were selected for chronological determinations of carbon isotope composition (δ13C) in tree-ring cellulose.
29579292	3	75	theme	isotope	518:524	arg1	composition					526:536	carbon isotope composition	511:536	carbon isotope composition (δ13C) in tree-ring cellulose	511:566	In this context, four beech forests along a latitudinal gradient from southern Apennines to middle European lowlands were selected for chronological determinations of carbon isotope composition (δ13C) in tree-ring cellulose.
29579292	4	76	from	effect	626:631	arg1	sites					982:986	different sites	972:986	different sites since 1950	972:997	The main objectives of this study were to assess (i) the effect of climate on the carbon signature of tree-ring cellulose (δ13C); (ii) the physiological response to recent CO2 concentration increment and to climatic variation; and (iii) the relationship between intrinsic water-use efficiency (iWUE, here the average long-term ratio of net photosynthesis to stomatal conductance) and growth of trees in different sites since 1950.
29579292	4	76	from	effect	626:631	arg1	signature					658:666	the carbon signature	647:666	the carbon signature of tree-ring cellulose (δ13C); (ii) the physiological response to recent CO2 concentration increment and to climatic variation; and (iii) the relationship between intrinsic water-use efficiency (iWUE, here the average long-term ratio of net photosynthesis to stomatal conductance	647:946	The main objectives of this study were to assess (i) the effect of climate on the carbon signature of tree-ring cellulose (δ13C); (ii) the physiological response to recent CO2 concentration increment and to climatic variation; and (iii) the relationship between intrinsic water-use efficiency (iWUE, here the average long-term ratio of net photosynthesis to stomatal conductance) and growth of trees in different sites since 1950.
29579292	11	77	theme	iWUE	2029:2032	arg1	iWUE					2029:2032	iWUE	2029:2032	iWUE	2029:2032	Overall, we observed a mean increment of 40% of iWUE.
29579292	11	77	theme	iWUE	2029:2032	arg1	%					2024:2024	40%	2022:2024	40% of iWUE	2022:2032	Overall, we observed a mean increment of 40% of iWUE.
29579292	11	78	theme	mean	2004:2007	arg1	increment					2009:2017	a mean increment	2002:2017	a mean increment of 40% of iWUE	2002:2032	Overall, we observed a mean increment of 40% of iWUE.
29579292	3	79	theme	tree-ring	548:556	arg1	cellulose					558:566	tree-ring cellulose	548:566	tree-ring cellulose	548:566	In this context, four beech forests along a latitudinal gradient from southern Apennines to middle European lowlands were selected for chronological determinations of carbon isotope composition (δ13C) in tree-ring cellulose.
29579292	4	80	dep	response	722:729	arg1	variation					785:793	climatic variation	776:793	climatic variation	776:793	The main objectives of this study were to assess (i) the effect of climate on the carbon signature of tree-ring cellulose (δ13C); (ii) the physiological response to recent CO2 concentration increment and to climatic variation; and (iii) the relationship between intrinsic water-use efficiency (iWUE, here the average long-term ratio of net photosynthesis to stomatal conductance) and growth of trees in different sites since 1950.
29579292	4	80	dep	response	722:729	arg1	ii					700:701	ii	700:701	ii	700:701	The main objectives of this study were to assess (i) the effect of climate on the carbon signature of tree-ring cellulose (δ13C); (ii) the physiological response to recent CO2 concentration increment and to climatic variation; and (iii) the relationship between intrinsic water-use efficiency (iWUE, here the average long-term ratio of net photosynthesis to stomatal conductance) and growth of trees in different sites since 1950.
29579292	7	81	theme	significant	1331:1341	arg1	relationship					1343:1354	a significant relationship	1329:1354	a significant relationship between spring temperature and δ13C in the coldest sites	1329:1411	Furthermore, an 'earliness effect' was suggested by a significant relationship between spring temperature and δ13C in the coldest sites and by a positive one between winter temperature and δ13C in the warmest ones.
29579292	9	82	theme	active	1772:1777	arg1	response					1779:1786	an active response	1769:1786	an active response of trees to CO2 fertilization	1769:1816	This increment of CO2 was the main driver of the long term increasing trend of iWUE, resulting by an active response of trees to CO2 fertilization.
29579292	4	83	theme	water-use	841:849	arg1	efficiency					851:860	intrinsic water-use efficiency	831:860	intrinsic water-use efficiency	831:860	The main objectives of this study were to assess (i) the effect of climate on the carbon signature of tree-ring cellulose (δ13C); (ii) the physiological response to recent CO2 concentration increment and to climatic variation; and (iii) the relationship between intrinsic water-use efficiency (iWUE, here the average long-term ratio of net photosynthesis to stomatal conductance) and growth of trees in different sites since 1950.
29579292	13	84	theme	silvicultural	2278:2290	arg1	treatments					2292:2301	silvicultural treatments	2278:2301	silvicultural treatments	2278:2301	Increasing iWUE was correlated to growth in the two sites during the release phase and we hypothesize a positive effect of silvicultural treatments.
29579292	2	85	theme	tree	198:201	arg1	species					203:209	the most important forest tree species	172:209	the most important forest tree species in Europe	172:219	Beech is one of the most important forest tree species in Europe, hence possible adverse factors affecting its physiology and productivity can have strong ecological and economic impacts.
29579292	8	86	theme	active	1628:1633	arg1	response					1635:1642	an active response	1625:1642	an active response of trees to CO2 increment	1625:1668	In all the study sites, during the maturity phase, a positive correlation between the increment of CO2 and iWUE was observed, due to an active response of trees to CO2 increment.
29579292	2	87	theme	possible	228:235	arg1	factors					245:251	possible adverse factors	228:251	possible adverse factors affecting its physiology and productivity	228:293	Beech is one of the most important forest tree species in Europe, hence possible adverse factors affecting its physiology and productivity can have strong ecological and economic impacts.
29579292	1	88	theme	carbon	117:122	arg1	analyses					132:139	carbon isotope analyses	117:139	carbon isotope analyses in tree rings	117:153	Evidences from carbon isotope analyses in tree rings.
29579292	6	89	theme	summer	1120:1125	arg1	precipitation					1127:1139	summer precipitation	1120:1139	summer precipitation	1120:1139	In northern sites, a climatic control of summer precipitation and temperature on stomatal conductance was demonstrated by their opposite correlations with δ13C, negative and positive, respectively.
29579292	4	90	theme	tree-ring	671:679	arg1	δ13C					692:695	δ13C	692:695	δ13C	692:695	The main objectives of this study were to assess (i) the effect of climate on the carbon signature of tree-ring cellulose (δ13C); (ii) the physiological response to recent CO2 concentration increment and to climatic variation; and (iii) the relationship between intrinsic water-use efficiency (iWUE, here the average long-term ratio of net photosynthesis to stomatal conductance) and growth of trees in different sites since 1950.
29579292	4	90	theme	tree-ring	671:679	arg1	cellulose					681:689	tree-ring cellulose	671:689	tree-ring cellulose (δ13C)	671:696	The main objectives of this study were to assess (i) the effect of climate on the carbon signature of tree-ring cellulose (δ13C); (ii) the physiological response to recent CO2 concentration increment and to climatic variation; and (iii) the relationship between intrinsic water-use efficiency (iWUE, here the average long-term ratio of net photosynthesis to stomatal conductance) and growth of trees in different sites since 1950.
29579292	4	91	from	sites	982:986	arg1	growth					953:958	growth	953:958	growth of trees in different sites since 1950	953:997	The main objectives of this study were to assess (i) the effect of climate on the carbon signature of tree-ring cellulose (δ13C); (ii) the physiological response to recent CO2 concentration increment and to climatic variation; and (iii) the relationship between intrinsic water-use efficiency (iWUE, here the average long-term ratio of net photosynthesis to stomatal conductance) and growth of trees in different sites since 1950.
29579292	4	91	from	sites	982:986	arg1	effect					626:631	the effect	622:631	(i) the effect of climate on the carbon signature of tree-ring cellulose (δ13C); (ii) the physiological response to recent CO2 concentration increment and to climatic variation; and (iii) the relationship between intrinsic water-use efficiency (iWUE, here the average long-term ratio of net photosynthesis to stomatal conductance)	618:947	The main objectives of this study were to assess (i) the effect of climate on the carbon signature of tree-ring cellulose (δ13C); (ii) the physiological response to recent CO2 concentration increment and to climatic variation; and (iii) the relationship between intrinsic water-use efficiency (iWUE, here the average long-term ratio of net photosynthesis to stomatal conductance) and growth of trees in different sites since 1950.
29579292	4	92	theme	long-term	886:894	arg1	ratio					896:900	the average long-term ratio	874:900	the average long-term ratio of net photosynthesis to stomatal conductance	874:946	The main objectives of this study were to assess (i) the effect of climate on the carbon signature of tree-ring cellulose (δ13C); (ii) the physiological response to recent CO2 concentration increment and to climatic variation; and (iii) the relationship between intrinsic water-use efficiency (iWUE, here the average long-term ratio of net photosynthesis to stomatal conductance) and growth of trees in different sites since 1950.
29579292	4	93	theme	climate	636:642	arg1	growth					953:958	growth	953:958	growth of trees in different sites since 1950	953:997	The main objectives of this study were to assess (i) the effect of climate on the carbon signature of tree-ring cellulose (δ13C); (ii) the physiological response to recent CO2 concentration increment and to climatic variation; and (iii) the relationship between intrinsic water-use efficiency (iWUE, here the average long-term ratio of net photosynthesis to stomatal conductance) and growth of trees in different sites since 1950.
29579292	4	93	theme	climate	636:642	arg1	effect					626:631	the effect	622:631	(i) the effect of climate on the carbon signature of tree-ring cellulose (δ13C); (ii) the physiological response to recent CO2 concentration increment and to climatic variation; and (iii) the relationship between intrinsic water-use efficiency (iWUE, here the average long-term ratio of net photosynthesis to stomatal conductance)	618:947	The main objectives of this study were to assess (i) the effect of climate on the carbon signature of tree-ring cellulose (δ13C); (ii) the physiological response to recent CO2 concentration increment and to climatic variation; and (iii) the relationship between intrinsic water-use efficiency (iWUE, here the average long-term ratio of net photosynthesis to stomatal conductance) and growth of trees in different sites since 1950.
29579292	4	94	dep	relationship	810:821	arg1	iii					801:803	iii	801:803	iii	801:803	The main objectives of this study were to assess (i) the effect of climate on the carbon signature of tree-ring cellulose (δ13C); (ii) the physiological response to recent CO2 concentration increment and to climatic variation; and (iii) the relationship between intrinsic water-use efficiency (iWUE, here the average long-term ratio of net photosynthesis to stomatal conductance) and growth of trees in different sites since 1950.
29579292	1	95	theme	tree	144:147	arg1	rings					149:153	tree rings	144:153	tree rings	144:153	Evidences from carbon isotope analyses in tree rings.
29579292	7	96	theme	warmest	1478:1484	arg1	ones					1486:1489	the warmest ones	1474:1489	the warmest ones	1474:1489	Furthermore, an 'earliness effect' was suggested by a significant relationship between spring temperature and δ13C in the coldest sites and by a positive one between winter temperature and δ13C in the warmest ones.
29579292	0	97	theme	common	88:93	arg1	beech					95:99	common beech	88:99	common beech	88:99	Do atmospheric CO2 concentration increase, climate and forest management affect iWUE of common beech?
29579292	12	98	theme	CO2	2088:2090	arg1	increase					2076:2083	the increase	2072:2083	the increase of CO2	2072:2090	Moreover, the sensitivity of iWUE to the increase of CO2 was different between the northernmost and southernmost sites.
29579292	12	99	theme	iWUE	2064:2067	arg1	sensitivity					2049:2059	the sensitivity	2045:2059	the sensitivity of iWUE to the increase of CO2	2045:2090	Moreover, the sensitivity of iWUE to the increase of CO2 was different between the northernmost and southernmost sites.
29579292	12	99	theme	iWUE	2064:2067	arg1	different					2096:2104	different	2096:2104	different	2096:2104	Moreover, the sensitivity of iWUE to the increase of CO2 was different between the northernmost and southernmost sites.
29579292	13	100	from	growth	2189:2194	arg1	sites					2207:2211	the two sites	2199:2211	the two sites	2199:2211	Increasing iWUE was correlated to growth in the two sites during the release phase and we hypothesize a positive effect of silvicultural treatments.
29579292	0	101	theme	atmospheric	3:13	arg1	increase					33:40	atmospheric CO2 concentration increase	3:40	atmospheric CO2 concentration increase	3:40	Do atmospheric CO2 concentration increase, climate and forest management affect iWUE of common beech?
29579292	5	102	theme	site	1030:1033	arg1	conditions					1044:1053	site climatic conditions	1030:1053	site climatic conditions	1030:1053	Our results demonstrated that site climatic conditions peculiarly affect δ13C.
29579292	2	103	from	species	203:209	arg1	Europe					214:219	Europe	214:219	Europe	214:219	Beech is one of the most important forest tree species in Europe, hence possible adverse factors affecting its physiology and productivity can have strong ecological and economic impacts.
29579292	11	104	theme	%	2024:2024	arg1	increment					2009:2017	a mean increment	2002:2017	a mean increment of 40% of iWUE	2002:2032	Overall, we observed a mean increment of 40% of iWUE.
29579292	0	105	theme	concentration	19:31	arg1	increase					33:40	atmospheric CO2 concentration increase	3:40	atmospheric CO2 concentration increase	3:40	Do atmospheric CO2 concentration increase, climate and forest management affect iWUE of common beech?
29579292	4	106	from	growth	953:958	arg1	sites					982:986	different sites	972:986	different sites since 1950	972:997	The main objectives of this study were to assess (i) the effect of climate on the carbon signature of tree-ring cellulose (δ13C); (ii) the physiological response to recent CO2 concentration increment and to climatic variation; and (iii) the relationship between intrinsic water-use efficiency (iWUE, here the average long-term ratio of net photosynthesis to stomatal conductance) and growth of trees in different sites since 1950.
29579292	4	106	from	growth	953:958	arg1	signature					658:666	the carbon signature	647:666	the carbon signature of tree-ring cellulose (δ13C); (ii) the physiological response to recent CO2 concentration increment and to climatic variation; and (iii) the relationship between intrinsic water-use efficiency (iWUE, here the average long-term ratio of net photosynthesis to stomatal conductance	647:946	The main objectives of this study were to assess (i) the effect of climate on the carbon signature of tree-ring cellulose (δ13C); (ii) the physiological response to recent CO2 concentration increment and to climatic variation; and (iii) the relationship between intrinsic water-use efficiency (iWUE, here the average long-term ratio of net photosynthesis to stomatal conductance) and growth of trees in different sites since 1950.
29579292	9	107	theme	CO2	1689:1691	arg1	increment					1676:1684	This increment	1671:1684	This increment of CO2	1671:1691	This increment of CO2 was the main driver of the long term increasing trend of iWUE, resulting by an active response of trees to CO2 fertilization.
29579292	9	107	theme	CO2	1689:1691	arg1	driver					1706:1711	the main driver	1697:1711	the main driver of the long term increasing trend of iWUE	1697:1753	This increment of CO2 was the main driver of the long term increasing trend of iWUE, resulting by an active response of trees to CO2 fertilization.
29579292	3	108	theme	European	443:450	arg1	lowlands					452:459	middle European lowlands	436:459	middle European lowlands	436:459	In this context, four beech forests along a latitudinal gradient from southern Apennines to middle European lowlands were selected for chronological determinations of carbon isotope composition (δ13C) in tree-ring cellulose.
29579292	10	109	theme	iWUE	1918:1921	arg1	variations					1904:1913	the inter-annual variations	1887:1913	the inter-annual variations of iWUE of the southernmost and northernmost sites, respectively	1887:1978	Moreover, precipitation mostly influences positively and negatively the inter-annual variations of iWUE of the southernmost and northernmost sites, respectively.
29579292	4	110	theme	photosynthesis	909:922	arg1	iWUE					863:866	iWUE	863:866	iWUE	863:866	The main objectives of this study were to assess (i) the effect of climate on the carbon signature of tree-ring cellulose (δ13C); (ii) the physiological response to recent CO2 concentration increment and to climatic variation; and (iii) the relationship between intrinsic water-use efficiency (iWUE, here the average long-term ratio of net photosynthesis to stomatal conductance) and growth of trees in different sites since 1950.
29579292	4	110	theme	photosynthesis	909:922	arg1	ratio					896:900	the average long-term ratio	874:900	the average long-term ratio of net photosynthesis to stomatal conductance	874:946	The main objectives of this study were to assess (i) the effect of climate on the carbon signature of tree-ring cellulose (δ13C); (ii) the physiological response to recent CO2 concentration increment and to climatic variation; and (iii) the relationship between intrinsic water-use efficiency (iWUE, here the average long-term ratio of net photosynthesis to stomatal conductance) and growth of trees in different sites since 1950.
29579292	4	110	theme	photosynthesis	909:922	arg1	cellulose					681:689	tree-ring cellulose	671:689	tree-ring cellulose (δ13C)	671:696	The main objectives of this study were to assess (i) the effect of climate on the carbon signature of tree-ring cellulose (δ13C); (ii) the physiological response to recent CO2 concentration increment and to climatic variation; and (iii) the relationship between intrinsic water-use efficiency (iWUE, here the average long-term ratio of net photosynthesis to stomatal conductance) and growth of trees in different sites since 1950.
29579292	4	110	theme	photosynthesis	909:922	arg1	relationship					810:821	the relationship	806:821	(iii) the relationship between intrinsic water-use efficiency	800:860	The main objectives of this study were to assess (i) the effect of climate on the carbon signature of tree-ring cellulose (δ13C); (ii) the physiological response to recent CO2 concentration increment and to climatic variation; and (iii) the relationship between intrinsic water-use efficiency (iWUE, here the average long-term ratio of net photosynthesis to stomatal conductance) and growth of trees in different sites since 1950.
29579292	4	110	theme	photosynthesis	909:922	arg1	δ13C					692:695	δ13C	692:695	δ13C	692:695	The main objectives of this study were to assess (i) the effect of climate on the carbon signature of tree-ring cellulose (δ13C); (ii) the physiological response to recent CO2 concentration increment and to climatic variation; and (iii) the relationship between intrinsic water-use efficiency (iWUE, here the average long-term ratio of net photosynthesis to stomatal conductance) and growth of trees in different sites since 1950.
29579292	4	110	theme	photosynthesis	909:922	arg1	response					722:729	the physiological response	704:729	(ii) the physiological response to recent CO2 concentration increment	699:767	The main objectives of this study were to assess (i) the effect of climate on the carbon signature of tree-ring cellulose (δ13C); (ii) the physiological response to recent CO2 concentration increment and to climatic variation; and (iii) the relationship between intrinsic water-use efficiency (iWUE, here the average long-term ratio of net photosynthesis to stomatal conductance) and growth of trees in different sites since 1950.
29579292	4	111	theme	CO2	741:743	arg1	increment					759:767	recent CO2 concentration increment	734:767	recent CO2 concentration increment	734:767	The main objectives of this study were to assess (i) the effect of climate on the carbon signature of tree-ring cellulose (δ13C); (ii) the physiological response to recent CO2 concentration increment and to climatic variation; and (iii) the relationship between intrinsic water-use efficiency (iWUE, here the average long-term ratio of net photosynthesis to stomatal conductance) and growth of trees in different sites since 1950.
29579292	3	112	from	Apennines	423:431	arg1	gradient					400:407	a latitudinal gradient	386:407	a latitudinal gradient from southern Apennines to middle European lowlands	386:459	In this context, four beech forests along a latitudinal gradient from southern Apennines to middle European lowlands were selected for chronological determinations of carbon isotope composition (δ13C) in tree-ring cellulose.
29579292	4	113	theme	stomatal	927:934	arg1	conductance					936:946	stomatal conductance	927:946	stomatal conductance	927:946	The main objectives of this study were to assess (i) the effect of climate on the carbon signature of tree-ring cellulose (δ13C); (ii) the physiological response to recent CO2 concentration increment and to climatic variation; and (iii) the relationship between intrinsic water-use efficiency (iWUE, here the average long-term ratio of net photosynthesis to stomatal conductance) and growth of trees in different sites since 1950.
29579292	2	114	theme	important	181:189	arg1	species					203:209	the most important forest tree species	172:209	the most important forest tree species in Europe	172:219	Beech is one of the most important forest tree species in Europe, hence possible adverse factors affecting its physiology and productivity can have strong ecological and economic impacts.
29579292	2	115	theme	ecological	311:320	arg1	impacts					335:341	strong ecological and economic impacts	304:341	strong ecological and economic impacts	304:341	Beech is one of the most important forest tree species in Europe, hence possible adverse factors affecting its physiology and productivity can have strong ecological and economic impacts.
29579292	8	116	theme	positive	1545:1552	arg1	correlation					1554:1564	a positive correlation	1543:1564	a positive correlation between the increment of CO2 and iWUE	1543:1602	In all the study sites, during the maturity phase, a positive correlation between the increment of CO2 and iWUE was observed, due to an active response of trees to CO2 increment.
29579292	4	117	theme	physiological	708:720	arg1	response					722:729	the physiological response	704:729	(ii) the physiological response to recent CO2 concentration increment	699:767	The main objectives of this study were to assess (i) the effect of climate on the carbon signature of tree-ring cellulose (δ13C); (ii) the physiological response to recent CO2 concentration increment and to climatic variation; and (iii) the relationship between intrinsic water-use efficiency (iWUE, here the average long-term ratio of net photosynthesis to stomatal conductance) and growth of trees in different sites since 1950.
29579292	7	118	theme	positive	1422:1429	arg1	one					1431:1433	a positive one	1420:1433	a positive one between winter temperature and δ13C in the warmest ones	1420:1489	Furthermore, an 'earliness effect' was suggested by a significant relationship between spring temperature and δ13C in the coldest sites and by a positive one between winter temperature and δ13C in the warmest ones.
29579292	9	119	theme	term	1725:1728	arg1	trend					1741:1745	the long term increasing trend	1716:1745	the long term increasing trend of iWUE	1716:1753	This increment of CO2 was the main driver of the long term increasing trend of iWUE, resulting by an active response of trees to CO2 fertilization.
29579292	10	120	theme	sites	1960:1964	arg1	iWUE					1918:1921	iWUE	1918:1921	iWUE	1918:1921	Moreover, precipitation mostly influences positively and negatively the inter-annual variations of iWUE of the southernmost and northernmost sites, respectively.
29579292	3	121	theme	carbon	511:516	arg1	δ13C					539:542	δ13C	539:542	δ13C	539:542	In this context, four beech forests along a latitudinal gradient from southern Apennines to middle European lowlands were selected for chronological determinations of carbon isotope composition (δ13C) in tree-ring cellulose.
29579292	3	121	theme	carbon	511:516	arg1	composition					526:536	carbon isotope composition	511:536	carbon isotope composition (δ13C) in tree-ring cellulose	511:566	In this context, four beech forests along a latitudinal gradient from southern Apennines to middle European lowlands were selected for chronological determinations of carbon isotope composition (δ13C) in tree-ring cellulose.
31557180	6	0	theme	SCW	1058:1060	arg1	cells					1062:1066	SCW cells	1058:1066	SCW cells	1058:1066	The distinctive banding found in many rhodoliths is the regular transition from PCW-only cells to SCW cells.
31557180	6	1	from	cells	1049:1053	arg1	banding					976:982	The distinctive banding	960:982	The distinctive banding found in many rhodoliths	960:1007	The distinctive banding found in many rhodoliths is the regular transition from PCW-only cells to SCW cells.
31557180	6	1	from	cells	1049:1053	arg1	transition					1024:1033	the regular transition	1012:1033	the regular transition from PCW-only cells to SCW cells	1012:1066	The distinctive banding found in many rhodoliths is the regular transition from PCW-only cells to SCW cells.
31557180	9	2	theme	thin	1330:1333	arg1	PCW					1346:1348	A thin (< 0.5 μm) PCW	1328:1348	A thin (< 0.5 μm) PCW	1328:1348	1) A thin (< 0.5 μm) PCW forms and is filled with a mineralising fluid of organic compounds and seawater.
31557180	10	3	theme	Nanometer-scale	1431:1445	arg1	grains					1458:1463	Nanometer-scale Mg-calcite grains	1431:1463	Nanometer-scale Mg-calcite grains	1431:1463	Nanometer-scale Mg-calcite grains precipitate on the organic structures within the PCW.
31557180	5	4	theme	primary	617:623	arg1	PCW					636:638	PCW	636:638	PCW	636:638	We describe two types of cell wall calcification; 1) calcified primary cell wall (PCW) in the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA), 2) calcified secondary cell wall (SCW) with radial Mg-calcite crystals in thicker-walled rounded cortical cells of articulated corallines and perithallial cells of CCA.
31557180	5	4	theme	primary	617:623	arg1	wall					630:633	primary cell wall	617:633	1) calcified primary cell wall (PCW) in the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA)	604:788	We describe two types of cell wall calcification; 1) calcified primary cell wall (PCW) in the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA), 2) calcified secondary cell wall (SCW) with radial Mg-calcite crystals in thicker-walled rounded cortical cells of articulated corallines and perithallial cells of CCA.
31557180	2	5	theme	rhodolith	279:287	arg1	beds					289:292	rhodolith beds	279:292	rhodolith beds	279:292	Their capacity to build enduring crusts that underpin the formation of tropical reefs, rhodolith beds and other benthic substrate is dependent on the formation of a calcified thallus.
31557180	10	6	theme	organic	1484:1490	arg1	structures					1492:1501	the organic structures	1480:1501	the organic structures within the PCW	1480:1516	Nanometer-scale Mg-calcite grains precipitate on the organic structures within the PCW.
31557180	13	7	theme	sub-surface	1919:1929	arg1	crust					1931:1935	the sub-surface crust	1915:1935	the sub-surface crust	1915:1935	In Clathromorphum, SCW formation lags PCW creating a zone of weakness resulting in a split in the sub-surface crust.
31557180	8	8	dep	propose	1267:1273	arg1	MODEL					1240:1244	MODEL	1240:1244	MODEL	1240:1244	MODEL FOR CALCIFICATION We propose the following three-step model for calcification.
31557180	7	9	with	Mg	1122:1123	arg1	content					1133:1139	Mg content	1130:1139	Mg content of a few mol% higher than radial Mg-calcite (M-type), ranging up to dolomite composition (D-type)	1130:1237	Within the cell walls there can be bands of elevated Mg with Mg content of a few mol% higher than radial Mg-calcite (M-type), ranging up to dolomite composition (D-type).
31557180	2	10	theme	benthic	304:310	arg1	substrate					312:320	other benthic substrate	298:320	other benthic substrate	298:320	Their capacity to build enduring crusts that underpin the formation of tropical reefs, rhodolith beds and other benthic substrate is dependent on the formation of a calcified thallus.
31557180	5	11	theme	calcified	794:802	arg1	types					570:574	two types	566:574	two types of cell wall calcification	566:601	We describe two types of cell wall calcification; 1) calcified primary cell wall (PCW) in the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA), 2) calcified secondary cell wall (SCW) with radial Mg-calcite crystals in thicker-walled rounded cortical cells of articulated corallines and perithallial cells of CCA.
31557180	5	11	theme	calcified	794:802	arg1	SCW					825:827	SCW	825:827	SCW	825:827	We describe two types of cell wall calcification; 1) calcified primary cell wall (PCW) in the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA), 2) calcified secondary cell wall (SCW) with radial Mg-calcite crystals in thicker-walled rounded cortical cells of articulated corallines and perithallial cells of CCA.
31557180	5	11	theme	calcified	794:802	arg1	wall					819:822	secondary cell wall	804:822	2) calcified secondary cell wall (SCW)	791:828	We describe two types of cell wall calcification; 1) calcified primary cell wall (PCW) in the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA), 2) calcified secondary cell wall (SCW) with radial Mg-calcite crystals in thicker-walled rounded cortical cells of articulated corallines and perithallial cells of CCA.
31557180	1	12	theme	key	130:132	arg1	FINDINGS					101:108	FINDINGS	101:108	FINDINGS	101:108	RESEARCH PURPOSE AND FINDINGS Coralline algae are key biological substrates of many carbonate systems globally.
31557180	1	12	theme	key	130:132	arg1	substrates					145:154	key biological substrates	130:154	key biological substrates of many carbonate systems	130:180	RESEARCH PURPOSE AND FINDINGS Coralline algae are key biological substrates of many carbonate systems globally.
31557180	1	12	theme	key	130:132	arg1	PURPOSE					89:95	RESEARCH PURPOSE	80:95	RESEARCH PURPOSE	80:95	RESEARCH PURPOSE AND FINDINGS Coralline algae are key biological substrates of many carbonate systems globally.
31557180	14	13	theme	bioinduced	1977:1986	arg1	process					2011:2017	a bioinduced rather than controlled process	1975:2017	a bioinduced rather than controlled process	1975:2017	All calcification seems likely to be a bioinduced rather than controlled process.
31557180	5	14	theme	medullary	691:699	arg1	cells					701:705	central medullary cells	683:705	central medullary cells in articulated corallines	683:731	We describe two types of cell wall calcification; 1) calcified primary cell wall (PCW) in the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA), 2) calcified secondary cell wall (SCW) with radial Mg-calcite crystals in thicker-walled rounded cortical cells of articulated corallines and perithallial cells of CCA.
31557180	5	15	from	cells	701:705	arg1	algae					778:782	crustose coralline algae	759:782	crustose coralline algae (CCA)	759:788	We describe two types of cell wall calcification; 1) calcified primary cell wall (PCW) in the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA), 2) calcified secondary cell wall (SCW) with radial Mg-calcite crystals in thicker-walled rounded cortical cells of articulated corallines and perithallial cells of CCA.
31557180	5	15	from	cells	701:705	arg1	corallines					722:731	articulated corallines	710:731	articulated corallines	710:731	We describe two types of cell wall calcification; 1) calcified primary cell wall (PCW) in the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA), 2) calcified secondary cell wall (SCW) with radial Mg-calcite crystals in thicker-walled rounded cortical cells of articulated corallines and perithallial cells of CCA.
31557180	5	15	from	cells	701:705	arg1	CCA					785:787	CCA	785:787	CCA	785:787	We describe two types of cell wall calcification; 1) calcified primary cell wall (PCW) in the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA), 2) calcified secondary cell wall (SCW) with radial Mg-calcite crystals in thicker-walled rounded cortical cells of articulated corallines and perithallial cells of CCA.
31557180	5	16	theme	cell	814:817	arg1	types					570:574	two types	566:574	two types of cell wall calcification	566:601	We describe two types of cell wall calcification; 1) calcified primary cell wall (PCW) in the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA), 2) calcified secondary cell wall (SCW) with radial Mg-calcite crystals in thicker-walled rounded cortical cells of articulated corallines and perithallial cells of CCA.
31557180	5	16	theme	cell	814:817	arg1	SCW					825:827	SCW	825:827	SCW	825:827	We describe two types of cell wall calcification; 1) calcified primary cell wall (PCW) in the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA), 2) calcified secondary cell wall (SCW) with radial Mg-calcite crystals in thicker-walled rounded cortical cells of articulated corallines and perithallial cells of CCA.
31557180	5	16	theme	cell	814:817	arg1	wall					819:822	secondary cell wall	804:822	2) calcified secondary cell wall (SCW)	791:828	We describe two types of cell wall calcification; 1) calcified primary cell wall (PCW) in the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA), 2) calcified secondary cell wall (SCW) with radial Mg-calcite crystals in thicker-walled rounded cortical cells of articulated corallines and perithallial cells of CCA.
31557180	12	17	theme	radial	1805:1810	arg1	calcite					1812:1818	the radial calcite	1801:1818	the radial calcite	1801:1818	3) The CMF soaks in the mineralising fluid as it extrudes and becomes calcified, retaining the perpendicular form, thus building the radial calcite.
31557180	5	18	theme	thin-walled	648:658	arg1	cells					701:705	central medullary cells	683:705	central medullary cells in articulated corallines	683:731	We describe two types of cell wall calcification; 1) calcified primary cell wall (PCW) in the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA), 2) calcified secondary cell wall (SCW) with radial Mg-calcite crystals in thicker-walled rounded cortical cells of articulated corallines and perithallial cells of CCA.
31557180	5	18	theme	thin-walled	648:658	arg1	cells					669:673	the thin-walled elongate cells	644:673	the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA)	644:788	We describe two types of cell wall calcification; 1) calcified primary cell wall (PCW) in the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA), 2) calcified secondary cell wall (SCW) with radial Mg-calcite crystals in thicker-walled rounded cortical cells of articulated corallines and perithallial cells of CCA.
31557180	5	18	theme	thin-walled	648:658	arg1	cells					750:754	hypothallial cells	737:754	hypothallial cells in crustose coralline algae (CCA)	737:788	We describe two types of cell wall calcification; 1) calcified primary cell wall (PCW) in the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA), 2) calcified secondary cell wall (SCW) with radial Mg-calcite crystals in thicker-walled rounded cortical cells of articulated corallines and perithallial cells of CCA.
31557180	5	19	from	cells	750:754	arg1	algae					778:782	crustose coralline algae	759:782	crustose coralline algae (CCA)	759:788	We describe two types of cell wall calcification; 1) calcified primary cell wall (PCW) in the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA), 2) calcified secondary cell wall (SCW) with radial Mg-calcite crystals in thicker-walled rounded cortical cells of articulated corallines and perithallial cells of CCA.
31557180	5	19	from	cells	750:754	arg1	corallines					722:731	articulated corallines	710:731	articulated corallines	710:731	We describe two types of cell wall calcification; 1) calcified primary cell wall (PCW) in the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA), 2) calcified secondary cell wall (SCW) with radial Mg-calcite crystals in thicker-walled rounded cortical cells of articulated corallines and perithallial cells of CCA.
31557180	5	19	from	cells	750:754	arg1	CCA					785:787	CCA	785:787	CCA	785:787	We describe two types of cell wall calcification; 1) calcified primary cell wall (PCW) in the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA), 2) calcified secondary cell wall (SCW) with radial Mg-calcite crystals in thicker-walled rounded cortical cells of articulated corallines and perithallial cells of CCA.
31557180	5	20	theme	thicker-walled	865:878	arg1	cells					897:901	thicker-walled rounded cortical cells	865:901	thicker-walled rounded cortical cells of articulated corallines	865:927	We describe two types of cell wall calcification; 1) calcified primary cell wall (PCW) in the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA), 2) calcified secondary cell wall (SCW) with radial Mg-calcite crystals in thicker-walled rounded cortical cells of articulated corallines and perithallial cells of CCA.
31557180	9	21	dep	thin	1330:1333	arg1	μm					1342:1343	< 0.5 μm	1336:1343	< 0.5 μm	1336:1343	1) A thin (< 0.5 μm) PCW forms and is filled with a mineralising fluid of organic compounds and seawater.
31557180	1	22	theme	Coralline	110:118	arg1	algae					120:124	Coralline algae	110:124	Coralline algae	110:124	RESEARCH PURPOSE AND FINDINGS Coralline algae are key biological substrates of many carbonate systems globally.
31557180	2	23	theme	reefs	272:276	arg1	formation					250:258	the formation	246:258	the formation of tropical reefs, rhodolith beds and other benthic substrate	246:320	Their capacity to build enduring crusts that underpin the formation of tropical reefs, rhodolith beds and other benthic substrate is dependent on the formation of a calcified thallus.
31557180	5	24	theme	cortical	888:895	arg1	cells					897:901	thicker-walled rounded cortical cells	865:901	thicker-walled rounded cortical cells of articulated corallines	865:927	We describe two types of cell wall calcification; 1) calcified primary cell wall (PCW) in the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA), 2) calcified secondary cell wall (SCW) with radial Mg-calcite crystals in thicker-walled rounded cortical cells of articulated corallines and perithallial cells of CCA.
31557180	5	25	theme	rounded	880:886	arg1	cells					897:901	thicker-walled rounded cortical cells	865:901	thicker-walled rounded cortical cells of articulated corallines	865:927	We describe two types of cell wall calcification; 1) calcified primary cell wall (PCW) in the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA), 2) calcified secondary cell wall (SCW) with radial Mg-calcite crystals in thicker-walled rounded cortical cells of articulated corallines and perithallial cells of CCA.
31557180	7	26	theme	dolomite	1209:1216	arg1	composition					1218:1228	dolomite composition	1209:1228	dolomite composition (D-type)	1209:1237	Within the cell walls there can be bands of elevated Mg with Mg content of a few mol% higher than radial Mg-calcite (M-type), ranging up to dolomite composition (D-type).
31557180	5	27	theme	hypothallial	737:748	arg1	cells					750:754	hypothallial cells	737:754	hypothallial cells in crustose coralline algae (CCA)	737:788	We describe two types of cell wall calcification; 1) calcified primary cell wall (PCW) in the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA), 2) calcified secondary cell wall (SCW) with radial Mg-calcite crystals in thicker-walled rounded cortical cells of articulated corallines and perithallial cells of CCA.
31557180	1	28	theme	many	159:162	arg1	systems					174:180	many carbonate systems	159:180	many carbonate systems	159:180	RESEARCH PURPOSE AND FINDINGS Coralline algae are key biological substrates of many carbonate systems globally.
31557180	4	29	theme	carbonate	497:505	arg1	features					507:514	cellular carbonate features	488:514	cellular carbonate features	488:514	We undertook a study of cellular carbonate features to develop a model for calcification.
31557180	7	30	theme	mol	1150:1152	arg1	%					1153:1153	few mol%	1146:1153	few mol%	1146:1153	Within the cell walls there can be bands of elevated Mg with Mg content of a few mol% higher than radial Mg-calcite (M-type), ranging up to dolomite composition (D-type).
31557180	5	31	theme	calcification	589:601	arg1	types					570:574	two types	566:574	two types of cell wall calcification	566:601	We describe two types of cell wall calcification; 1) calcified primary cell wall (PCW) in the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA), 2) calcified secondary cell wall (SCW) with radial Mg-calcite crystals in thicker-walled rounded cortical cells of articulated corallines and perithallial cells of CCA.
31557180	5	31	theme	calcification	589:601	arg1	wall					819:822	secondary cell wall	804:822	2) calcified secondary cell wall (SCW)	791:828	We describe two types of cell wall calcification; 1) calcified primary cell wall (PCW) in the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA), 2) calcified secondary cell wall (SCW) with radial Mg-calcite crystals in thicker-walled rounded cortical cells of articulated corallines and perithallial cells of CCA.
31557180	1	32	theme	systems	174:180	arg1	FINDINGS					101:108	FINDINGS	101:108	FINDINGS	101:108	RESEARCH PURPOSE AND FINDINGS Coralline algae are key biological substrates of many carbonate systems globally.
31557180	1	32	theme	systems	174:180	arg1	substrates					145:154	key biological substrates	130:154	key biological substrates of many carbonate systems	130:180	RESEARCH PURPOSE AND FINDINGS Coralline algae are key biological substrates of many carbonate systems globally.
31557180	1	32	theme	systems	174:180	arg1	PURPOSE					89:95	RESEARCH PURPOSE	80:95	RESEARCH PURPOSE	80:95	RESEARCH PURPOSE AND FINDINGS Coralline algae are key biological substrates of many carbonate systems globally.
31557180	12	33	dep	building	1792:1799	arg1	becomes					1734:1740	becomes	1734:1740	becomes	1734:1740	3) The CMF soaks in the mineralising fluid as it extrudes and becomes calcified, retaining the perpendicular form, thus building the radial calcite.
31557180	12	33	dep	building	1792:1799	arg1	3					1672:1672	3	1672:1672	3	1672:1672	3) The CMF soaks in the mineralising fluid as it extrudes and becomes calcified, retaining the perpendicular form, thus building the radial calcite.
31557180	12	33	dep	building	1792:1799	arg1	extrudes					1721:1728	extrudes	1721:1728	extrudes	1721:1728	3) The CMF soaks in the mineralising fluid as it extrudes and becomes calcified, retaining the perpendicular form, thus building the radial calcite.
31557180	5	34	theme	corallines	918:927	arg1	cells					897:901	thicker-walled rounded cortical cells	865:901	thicker-walled rounded cortical cells of articulated corallines	865:927	We describe two types of cell wall calcification; 1) calcified primary cell wall (PCW) in the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA), 2) calcified secondary cell wall (SCW) with radial Mg-calcite crystals in thicker-walled rounded cortical cells of articulated corallines and perithallial cells of CCA.
31557180	5	34	theme	corallines	918:927	arg1	cells					946:950	perithallial cells	933:950	perithallial cells of CCA	933:957	We describe two types of cell wall calcification; 1) calcified primary cell wall (PCW) in the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA), 2) calcified secondary cell wall (SCW) with radial Mg-calcite crystals in thicker-walled rounded cortical cells of articulated corallines and perithallial cells of CCA.
31557180	5	35	theme	perithallial	933:944	arg1	cells					946:950	perithallial cells	933:950	perithallial cells of CCA	933:957	We describe two types of cell wall calcification; 1) calcified primary cell wall (PCW) in the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA), 2) calcified secondary cell wall (SCW) with radial Mg-calcite crystals in thicker-walled rounded cortical cells of articulated corallines and perithallial cells of CCA.
31557180	0	36	theme	morphological	33:45	arg1	understanding					65:77	A morphological and process-based understanding	31:77	A morphological and process-based understanding	31:77	Coralline algal calcification: A morphological and process-based understanding.
31557180	6	37	theme	PCW-only	1040:1047	arg1	cells					1049:1053	PCW-only cells	1040:1053	PCW-only cells	1040:1053	The distinctive banding found in many rhodoliths is the regular transition from PCW-only cells to SCW cells.
31557180	3	38	theme	formation	430:438	arg1	process					400:406	this important process	385:406	this important process of skeletal carbonate formation	385:438	However, this important process of skeletal carbonate formation is not well understood.
31557180	0	39	theme	Coralline	0:8	arg1	calcification					16:28	Coralline algal calcification	0:28	Coralline algal calcification	0:28	Coralline algal calcification: A morphological and process-based understanding.
31557180	5	40	theme	cell	579:582	arg1	calcification					589:601	cell wall calcification	579:601	cell wall calcification	579:601	We describe two types of cell wall calcification; 1) calcified primary cell wall (PCW) in the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA), 2) calcified secondary cell wall (SCW) with radial Mg-calcite crystals in thicker-walled rounded cortical cells of articulated corallines and perithallial cells of CCA.
31557180	7	41	dep	radial	1167:1172	arg1	than					1162:1165	than	1162:1165	than	1162:1165	Within the cell walls there can be bands of elevated Mg with Mg content of a few mol% higher than radial Mg-calcite (M-type), ranging up to dolomite composition (D-type).
31557180	7	41	dep	radial	1167:1172	arg1	%					1153:1153	few mol%	1146:1153	few mol%	1146:1153	Within the cell walls there can be bands of elevated Mg with Mg content of a few mol% higher than radial Mg-calcite (M-type), ranging up to dolomite composition (D-type).
31557180	7	41	dep	radial	1167:1172	arg1	higher					1155:1160	higher	1155:1160	higher	1155:1160	Within the cell walls there can be bands of elevated Mg with Mg content of a few mol% higher than radial Mg-calcite (M-type), ranging up to dolomite composition (D-type).
31557180	8	42	theme	three-step	1289:1298	arg1	model					1300:1304	the following three-step model	1275:1304	the following three-step model for calcification	1275:1322	MODEL FOR CALCIFICATION We propose the following three-step model for calcification.
31557180	7	43	theme	elevated	1113:1120	arg1	Mg					1122:1123	elevated Mg	1113:1123	elevated Mg with Mg content of a few mol% higher than radial Mg-calcite (M-type), ranging up to dolomite composition (D-type)	1113:1237	Within the cell walls there can be bands of elevated Mg with Mg content of a few mol% higher than radial Mg-calcite (M-type), ranging up to dolomite composition (D-type).
31557180	0	44	dep	understanding	65:77	arg1	calcification					16:28	Coralline algal calcification	0:28	Coralline algal calcification	0:28	Coralline algal calcification: A morphological and process-based understanding.
31557180	3	45	theme	skeletal	411:418	arg1	formation					430:438	skeletal carbonate formation	411:438	skeletal carbonate formation	411:438	However, this important process of skeletal carbonate formation is not well understood.
31557180	5	46	theme	coralline	768:776	arg1	algae					778:782	crustose coralline algae	759:782	crustose coralline algae (CCA)	759:788	We describe two types of cell wall calcification; 1) calcified primary cell wall (PCW) in the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA), 2) calcified secondary cell wall (SCW) with radial Mg-calcite crystals in thicker-walled rounded cortical cells of articulated corallines and perithallial cells of CCA.
31557180	5	46	theme	coralline	768:776	arg1	CCA					785:787	CCA	785:787	CCA	785:787	We describe two types of cell wall calcification; 1) calcified primary cell wall (PCW) in the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA), 2) calcified secondary cell wall (SCW) with radial Mg-calcite crystals in thicker-walled rounded cortical cells of articulated corallines and perithallial cells of CCA.
31557180	11	47	dep	microfibrils	1544:1555	arg1	2					1519:1519	2	1519:1519	2	1519:1519	2) Crystalline cellulose microfibrils (CMF) are extruded perpendicularly from the cellulose synthase complexes (CSC) in the plasmalemma to form the SCW.
31557180	3	48	theme	carbonate	420:428	arg1	formation					430:438	skeletal carbonate formation	411:438	skeletal carbonate formation	411:438	However, this important process of skeletal carbonate formation is not well understood.
31557180	5	49	dep	wall	819:822	arg1	2					791:791	2	791:791	2	791:791	We describe two types of cell wall calcification; 1) calcified primary cell wall (PCW) in the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA), 2) calcified secondary cell wall (SCW) with radial Mg-calcite crystals in thicker-walled rounded cortical cells of articulated corallines and perithallial cells of CCA.
31557180	2	50	theme	enduring	216:223	arg1	crusts					225:230	enduring crusts	216:230	enduring crusts that underpin the formation of tropical reefs, rhodolith beds and other benthic substrate	216:320	Their capacity to build enduring crusts that underpin the formation of tropical reefs, rhodolith beds and other benthic substrate is dependent on the formation of a calcified thallus.
31557180	9	51	dep	forms	1350:1354	arg1	1					1325:1325	1	1325:1325	1	1325:1325	1) A thin (< 0.5 μm) PCW forms and is filled with a mineralising fluid of organic compounds and seawater.
31557180	12	52	theme	mineralising	1696:1707	arg1	fluid					1709:1713	the mineralising fluid	1692:1713	the mineralising fluid	1692:1713	3) The CMF soaks in the mineralising fluid as it extrudes and becomes calcified, retaining the perpendicular form, thus building the radial calcite.
31557180	5	53	theme	radial	835:840	arg1	crystals					853:860	radial Mg-calcite crystals	835:860	radial Mg-calcite crystals in thicker-walled rounded cortical cells of articulated corallines and perithallial cells of CCA	835:957	We describe two types of cell wall calcification; 1) calcified primary cell wall (PCW) in the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA), 2) calcified secondary cell wall (SCW) with radial Mg-calcite crystals in thicker-walled rounded cortical cells of articulated corallines and perithallial cells of CCA.
31557180	11	54	theme	cellulose	1601:1609	arg1	CSC					1631:1633	CSC	1631:1633	CSC	1631:1633	2) Crystalline cellulose microfibrils (CMF) are extruded perpendicularly from the cellulose synthase complexes (CSC) in the plasmalemma to form the SCW.
31557180	11	54	theme	cellulose	1601:1609	arg1	complexes					1620:1628	the cellulose synthase complexes	1597:1628	the cellulose synthase complexes (CSC)	1597:1634	2) Crystalline cellulose microfibrils (CMF) are extruded perpendicularly from the cellulose synthase complexes (CSC) in the plasmalemma to form the SCW.
31557180	13	55	theme	SCW	1840:1842	arg1	formation					1844:1852	SCW formation	1840:1852	SCW formation	1840:1852	In Clathromorphum, SCW formation lags PCW creating a zone of weakness resulting in a split in the sub-surface crust.
31557180	7	56	dep	composition	1218:1228	arg1	D-type					1231:1236	D-type	1231:1236	D-type	1231:1236	Within the cell walls there can be bands of elevated Mg with Mg content of a few mol% higher than radial Mg-calcite (M-type), ranging up to dolomite composition (D-type).
31557180	7	56	dep	composition	1218:1228	arg1	up					1203:1204	up	1203:1204	up	1203:1204	Within the cell walls there can be bands of elevated Mg with Mg content of a few mol% higher than radial Mg-calcite (M-type), ranging up to dolomite composition (D-type).
31557180	11	57	theme	cellulose	1534:1542	arg1	CMF					1558:1560	CMF	1558:1560	CMF	1558:1560	2) Crystalline cellulose microfibrils (CMF) are extruded perpendicularly from the cellulose synthase complexes (CSC) in the plasmalemma to form the SCW.
31557180	11	57	theme	cellulose	1534:1542	arg1	microfibrils					1544:1555	2) Crystalline cellulose microfibrils	1519:1555	2) Crystalline cellulose microfibrils (CMF)	1519:1561	2) Crystalline cellulose microfibrils (CMF) are extruded perpendicularly from the cellulose synthase complexes (CSC) in the plasmalemma to form the SCW.
31557180	7	58	theme	cell	1080:1083	arg1	walls					1085:1089	the cell walls	1076:1089	the cell walls	1076:1089	Within the cell walls there can be bands of elevated Mg with Mg content of a few mol% higher than radial Mg-calcite (M-type), ranging up to dolomite composition (D-type).
31557180	2	59	theme	calcified	357:365	arg1	thallus					367:373	a calcified thallus	355:373	a calcified thallus	355:373	Their capacity to build enduring crusts that underpin the formation of tropical reefs, rhodolith beds and other benthic substrate is dependent on the formation of a calcified thallus.
31557180	7	60	theme	radial	1167:1172	arg1	Mg-calcite					1174:1183	a few mol% higher than radial Mg-calcite	1144:1183	a few mol% higher than radial Mg-calcite (M-type)	1144:1192	Within the cell walls there can be bands of elevated Mg with Mg content of a few mol% higher than radial Mg-calcite (M-type), ranging up to dolomite composition (D-type).
31557180	7	60	theme	radial	1167:1172	arg1	M-type					1186:1191	M-type	1186:1191	M-type	1186:1191	Within the cell walls there can be bands of elevated Mg with Mg content of a few mol% higher than radial Mg-calcite (M-type), ranging up to dolomite composition (D-type).
31557180	5	61	dep	types	570:574	arg1	PCW					636:638	PCW	636:638	PCW	636:638	We describe two types of cell wall calcification; 1) calcified primary cell wall (PCW) in the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA), 2) calcified secondary cell wall (SCW) with radial Mg-calcite crystals in thicker-walled rounded cortical cells of articulated corallines and perithallial cells of CCA.
31557180	5	61	dep	types	570:574	arg1	wall					630:633	primary cell wall	617:633	1) calcified primary cell wall (PCW) in the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA)	604:788	We describe two types of cell wall calcification; 1) calcified primary cell wall (PCW) in the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA), 2) calcified secondary cell wall (SCW) with radial Mg-calcite crystals in thicker-walled rounded cortical cells of articulated corallines and perithallial cells of CCA.
31557180	2	62	theme	other	298:302	arg1	substrate					312:320	other benthic substrate	298:320	other benthic substrate	298:320	Their capacity to build enduring crusts that underpin the formation of tropical reefs, rhodolith beds and other benthic substrate is dependent on the formation of a calcified thallus.
31557180	5	63	theme	Mg-calcite	842:851	arg1	crystals					853:860	radial Mg-calcite crystals	835:860	radial Mg-calcite crystals in thicker-walled rounded cortical cells of articulated corallines and perithallial cells of CCA	835:957	We describe two types of cell wall calcification; 1) calcified primary cell wall (PCW) in the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA), 2) calcified secondary cell wall (SCW) with radial Mg-calcite crystals in thicker-walled rounded cortical cells of articulated corallines and perithallial cells of CCA.
31557180	10	64	theme	Mg-calcite	1447:1456	arg1	grains					1458:1463	Nanometer-scale Mg-calcite grains	1431:1463	Nanometer-scale Mg-calcite grains	1431:1463	Nanometer-scale Mg-calcite grains precipitate on the organic structures within the PCW.
31557180	5	65	theme	calcified	607:615	arg1	PCW					636:638	PCW	636:638	PCW	636:638	We describe two types of cell wall calcification; 1) calcified primary cell wall (PCW) in the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA), 2) calcified secondary cell wall (SCW) with radial Mg-calcite crystals in thicker-walled rounded cortical cells of articulated corallines and perithallial cells of CCA.
31557180	5	65	theme	calcified	607:615	arg1	wall					630:633	primary cell wall	617:633	1) calcified primary cell wall (PCW) in the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA)	604:788	We describe two types of cell wall calcification; 1) calcified primary cell wall (PCW) in the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA), 2) calcified secondary cell wall (SCW) with radial Mg-calcite crystals in thicker-walled rounded cortical cells of articulated corallines and perithallial cells of CCA.
31557180	2	66	theme	beds	289:292	arg1	formation					250:258	the formation	246:258	the formation of tropical reefs, rhodolith beds and other benthic substrate	246:320	Their capacity to build enduring crusts that underpin the formation of tropical reefs, rhodolith beds and other benthic substrate is dependent on the formation of a calcified thallus.
31557180	15	67	theme	substantial	2041:2051	arg1	step					2053:2056	a substantial step	2039:2056	a substantial step forward in understanding how corallines calcify	2039:2104	These findings are a substantial step forward in understanding how corallines calcify.
31557180	15	67	theme	substantial	2041:2051	arg1	findings					2026:2033	These findings	2020:2033	These findings	2020:2033	These findings are a substantial step forward in understanding how corallines calcify.
31557180	5	68	theme	cell	625:628	arg1	PCW					636:638	PCW	636:638	PCW	636:638	We describe two types of cell wall calcification; 1) calcified primary cell wall (PCW) in the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA), 2) calcified secondary cell wall (SCW) with radial Mg-calcite crystals in thicker-walled rounded cortical cells of articulated corallines and perithallial cells of CCA.
31557180	5	68	theme	cell	625:628	arg1	wall					630:633	primary cell wall	617:633	1) calcified primary cell wall (PCW) in the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA)	604:788	We describe two types of cell wall calcification; 1) calcified primary cell wall (PCW) in the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA), 2) calcified secondary cell wall (SCW) with radial Mg-calcite crystals in thicker-walled rounded cortical cells of articulated corallines and perithallial cells of CCA.
31557180	14	69	theme	controlled	2000:2009	arg1	process					2011:2017	a bioinduced rather than controlled process	1975:2017	a bioinduced rather than controlled process	1975:2017	All calcification seems likely to be a bioinduced rather than controlled process.
31557180	2	70	theme	substrate	312:320	arg1	formation					250:258	the formation	246:258	the formation of tropical reefs, rhodolith beds and other benthic substrate	246:320	Their capacity to build enduring crusts that underpin the formation of tropical reefs, rhodolith beds and other benthic substrate is dependent on the formation of a calcified thallus.
31557180	9	71	theme	organic	1399:1405	arg1	compounds					1407:1415	organic compounds	1399:1415	organic compounds	1399:1415	1) A thin (< 0.5 μm) PCW forms and is filled with a mineralising fluid of organic compounds and seawater.
31557180	5	72	theme	central	683:689	arg1	cells					701:705	central medullary cells	683:705	central medullary cells in articulated corallines	683:731	We describe two types of cell wall calcification; 1) calcified primary cell wall (PCW) in the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA), 2) calcified secondary cell wall (SCW) with radial Mg-calcite crystals in thicker-walled rounded cortical cells of articulated corallines and perithallial cells of CCA.
31557180	5	73	theme	secondary	804:812	arg1	types					570:574	two types	566:574	two types of cell wall calcification	566:601	We describe two types of cell wall calcification; 1) calcified primary cell wall (PCW) in the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA), 2) calcified secondary cell wall (SCW) with radial Mg-calcite crystals in thicker-walled rounded cortical cells of articulated corallines and perithallial cells of CCA.
31557180	5	73	theme	secondary	804:812	arg1	SCW					825:827	SCW	825:827	SCW	825:827	We describe two types of cell wall calcification; 1) calcified primary cell wall (PCW) in the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA), 2) calcified secondary cell wall (SCW) with radial Mg-calcite crystals in thicker-walled rounded cortical cells of articulated corallines and perithallial cells of CCA.
31557180	5	73	theme	secondary	804:812	arg1	wall					819:822	secondary cell wall	804:822	2) calcified secondary cell wall (SCW)	791:828	We describe two types of cell wall calcification; 1) calcified primary cell wall (PCW) in the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA), 2) calcified secondary cell wall (SCW) with radial Mg-calcite crystals in thicker-walled rounded cortical cells of articulated corallines and perithallial cells of CCA.
31557180	15	74	theme	forward	2058:2064	arg1	step					2053:2056	a substantial step	2039:2056	a substantial step forward in understanding how corallines calcify	2039:2104	These findings are a substantial step forward in understanding how corallines calcify.
31557180	15	74	theme	forward	2058:2064	arg1	findings					2026:2033	These findings	2020:2033	These findings	2020:2033	These findings are a substantial step forward in understanding how corallines calcify.
31557180	1	75	dep	PURPOSE	89:95	arg1	algae					120:124	Coralline algae	110:124	Coralline algae	110:124	RESEARCH PURPOSE AND FINDINGS Coralline algae are key biological substrates of many carbonate systems globally.
31557180	5	76	theme	elongate	660:667	arg1	cells					701:705	central medullary cells	683:705	central medullary cells in articulated corallines	683:731	We describe two types of cell wall calcification; 1) calcified primary cell wall (PCW) in the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA), 2) calcified secondary cell wall (SCW) with radial Mg-calcite crystals in thicker-walled rounded cortical cells of articulated corallines and perithallial cells of CCA.
31557180	5	76	theme	elongate	660:667	arg1	cells					669:673	the thin-walled elongate cells	644:673	the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA)	644:788	We describe two types of cell wall calcification; 1) calcified primary cell wall (PCW) in the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA), 2) calcified secondary cell wall (SCW) with radial Mg-calcite crystals in thicker-walled rounded cortical cells of articulated corallines and perithallial cells of CCA.
31557180	5	76	theme	elongate	660:667	arg1	cells					750:754	hypothallial cells	737:754	hypothallial cells in crustose coralline algae (CCA)	737:788	We describe two types of cell wall calcification; 1) calcified primary cell wall (PCW) in the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA), 2) calcified secondary cell wall (SCW) with radial Mg-calcite crystals in thicker-walled rounded cortical cells of articulated corallines and perithallial cells of CCA.
31557180	1	77	theme	biological	134:143	arg1	FINDINGS					101:108	FINDINGS	101:108	FINDINGS	101:108	RESEARCH PURPOSE AND FINDINGS Coralline algae are key biological substrates of many carbonate systems globally.
31557180	1	77	theme	biological	134:143	arg1	substrates					145:154	key biological substrates	130:154	key biological substrates of many carbonate systems	130:180	RESEARCH PURPOSE AND FINDINGS Coralline algae are key biological substrates of many carbonate systems globally.
31557180	1	77	theme	biological	134:143	arg1	PURPOSE					89:95	RESEARCH PURPOSE	80:95	RESEARCH PURPOSE	80:95	RESEARCH PURPOSE AND FINDINGS Coralline algae are key biological substrates of many carbonate systems globally.
31557180	6	78	theme	many	993:996	arg1	rhodoliths					998:1007	many rhodoliths	993:1007	many rhodoliths	993:1007	The distinctive banding found in many rhodoliths is the regular transition from PCW-only cells to SCW cells.
31557180	2	79	theme	tropical	263:270	arg1	reefs					272:276	tropical reefs	263:276	tropical reefs	263:276	Their capacity to build enduring crusts that underpin the formation of tropical reefs, rhodolith beds and other benthic substrate is dependent on the formation of a calcified thallus.
31557180	9	80	theme	mineralising	1377:1388	arg1	fluid					1390:1394	a mineralising fluid	1375:1394	a mineralising fluid of organic compounds and seawater	1375:1428	1) A thin (< 0.5 μm) PCW forms and is filled with a mineralising fluid of organic compounds and seawater.
31557180	4	81	theme	features	507:514	arg1	study					479:483	a study	477:483	a study of cellular carbonate features to develop a model for calcification	477:551	We undertook a study of cellular carbonate features to develop a model for calcification.
31557180	7	82	theme	few	1146:1148	arg1	%					1153:1153	few mol%	1146:1153	few mol%	1146:1153	Within the cell walls there can be bands of elevated Mg with Mg content of a few mol% higher than radial Mg-calcite (M-type), ranging up to dolomite composition (D-type).
31557180	12	83	theme	perpendicular	1767:1779	arg1	form					1781:1784	the perpendicular form	1763:1784	the perpendicular form	1763:1784	3) The CMF soaks in the mineralising fluid as it extrudes and becomes calcified, retaining the perpendicular form, thus building the radial calcite.
31557180	1	84	theme	carbonate	164:172	arg1	systems					174:180	many carbonate systems	159:180	many carbonate systems	159:180	RESEARCH PURPOSE AND FINDINGS Coralline algae are key biological substrates of many carbonate systems globally.
31557180	6	85	theme	distinctive	964:974	arg1	transition					1024:1033	the regular transition	1012:1033	the regular transition from PCW-only cells to SCW cells	1012:1066	The distinctive banding found in many rhodoliths is the regular transition from PCW-only cells to SCW cells.
31557180	6	85	theme	distinctive	964:974	arg1	banding					976:982	The distinctive banding	960:982	The distinctive banding found in many rhodoliths	960:1007	The distinctive banding found in many rhodoliths is the regular transition from PCW-only cells to SCW cells.
31557180	8	86	theme	following	1279:1287	arg1	model					1300:1304	the following three-step model	1275:1304	the following three-step model for calcification	1275:1322	MODEL FOR CALCIFICATION We propose the following three-step model for calcification.
31557180	5	87	from	crystals	853:860	arg1	cells					897:901	thicker-walled rounded cortical cells	865:901	thicker-walled rounded cortical cells of articulated corallines	865:927	We describe two types of cell wall calcification; 1) calcified primary cell wall (PCW) in the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA), 2) calcified secondary cell wall (SCW) with radial Mg-calcite crystals in thicker-walled rounded cortical cells of articulated corallines and perithallial cells of CCA.
31557180	5	87	from	crystals	853:860	arg1	cells					946:950	perithallial cells	933:950	perithallial cells of CCA	933:957	We describe two types of cell wall calcification; 1) calcified primary cell wall (PCW) in the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA), 2) calcified secondary cell wall (SCW) with radial Mg-calcite crystals in thicker-walled rounded cortical cells of articulated corallines and perithallial cells of CCA.
31557180	9	88	theme	compounds	1407:1415	arg1	fluid					1390:1394	a mineralising fluid	1375:1394	a mineralising fluid of organic compounds and seawater	1375:1428	1) A thin (< 0.5 μm) PCW forms and is filled with a mineralising fluid of organic compounds and seawater.
31557180	5	89	theme	articulated	710:720	arg1	corallines					722:731	articulated corallines	710:731	articulated corallines	710:731	We describe two types of cell wall calcification; 1) calcified primary cell wall (PCW) in the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA), 2) calcified secondary cell wall (SCW) with radial Mg-calcite crystals in thicker-walled rounded cortical cells of articulated corallines and perithallial cells of CCA.
31557180	13	90	theme	weakness	1882:1889	arg1	zone					1874:1877	a zone	1872:1877	a zone of weakness resulting in a split in the sub-surface crust	1872:1935	In Clathromorphum, SCW formation lags PCW creating a zone of weakness resulting in a split in the sub-surface crust.
31557180	1	91	theme	RESEARCH	80:87	arg1	FINDINGS					101:108	FINDINGS	101:108	FINDINGS	101:108	RESEARCH PURPOSE AND FINDINGS Coralline algae are key biological substrates of many carbonate systems globally.
31557180	1	91	theme	RESEARCH	80:87	arg1	PURPOSE					89:95	RESEARCH PURPOSE	80:95	RESEARCH PURPOSE	80:95	RESEARCH PURPOSE AND FINDINGS Coralline algae are key biological substrates of many carbonate systems globally.
31557180	1	91	theme	RESEARCH	80:87	arg1	substrates					145:154	key biological substrates	130:154	key biological substrates of many carbonate systems	130:180	RESEARCH PURPOSE AND FINDINGS Coralline algae are key biological substrates of many carbonate systems globally.
31557180	4	92	theme	cellular	488:495	arg1	features					507:514	cellular carbonate features	488:514	cellular carbonate features	488:514	We undertook a study of cellular carbonate features to develop a model for calcification.
31557180	7	93	theme	Mg	1130:1131	arg1	content					1133:1139	Mg content	1130:1139	Mg content of a few mol% higher than radial Mg-calcite (M-type), ranging up to dolomite composition (D-type)	1130:1237	Within the cell walls there can be bands of elevated Mg with Mg content of a few mol% higher than radial Mg-calcite (M-type), ranging up to dolomite composition (D-type).
31557180	0	94	theme	algal	10:14	arg1	calcification					16:28	Coralline algal calcification	0:28	Coralline algal calcification	0:28	Coralline algal calcification: A morphological and process-based understanding.
31557180	3	95	theme	important	390:398	arg1	process					400:406	this important process	385:406	this important process of skeletal carbonate formation	385:438	However, this important process of skeletal carbonate formation is not well understood.
31557180	5	96	from	wall	630:633	arg1	cells					701:705	central medullary cells	683:705	central medullary cells in articulated corallines	683:731	We describe two types of cell wall calcification; 1) calcified primary cell wall (PCW) in the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA), 2) calcified secondary cell wall (SCW) with radial Mg-calcite crystals in thicker-walled rounded cortical cells of articulated corallines and perithallial cells of CCA.
31557180	5	96	from	wall	630:633	arg1	cells					669:673	the thin-walled elongate cells	644:673	the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA)	644:788	We describe two types of cell wall calcification; 1) calcified primary cell wall (PCW) in the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA), 2) calcified secondary cell wall (SCW) with radial Mg-calcite crystals in thicker-walled rounded cortical cells of articulated corallines and perithallial cells of CCA.
31557180	5	96	from	wall	630:633	arg1	cells					750:754	hypothallial cells	737:754	hypothallial cells in crustose coralline algae (CCA)	737:788	We describe two types of cell wall calcification; 1) calcified primary cell wall (PCW) in the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA), 2) calcified secondary cell wall (SCW) with radial Mg-calcite crystals in thicker-walled rounded cortical cells of articulated corallines and perithallial cells of CCA.
31557180	5	97	theme	wall	584:587	arg1	calcification					589:601	cell wall calcification	579:601	cell wall calcification	579:601	We describe two types of cell wall calcification; 1) calcified primary cell wall (PCW) in the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA), 2) calcified secondary cell wall (SCW) with radial Mg-calcite crystals in thicker-walled rounded cortical cells of articulated corallines and perithallial cells of CCA.
31557180	5	98	theme	articulated	906:916	arg1	corallines					918:927	articulated corallines	906:927	articulated corallines	906:927	We describe two types of cell wall calcification; 1) calcified primary cell wall (PCW) in the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA), 2) calcified secondary cell wall (SCW) with radial Mg-calcite crystals in thicker-walled rounded cortical cells of articulated corallines and perithallial cells of CCA.
31557180	6	99	theme	regular	1016:1022	arg1	banding					976:982	The distinctive banding	960:982	The distinctive banding found in many rhodoliths	960:1007	The distinctive banding found in many rhodoliths is the regular transition from PCW-only cells to SCW cells.
31557180	6	99	theme	regular	1016:1022	arg1	transition					1024:1033	the regular transition	1012:1033	the regular transition from PCW-only cells to SCW cells	1012:1066	The distinctive banding found in many rhodoliths is the regular transition from PCW-only cells to SCW cells.
31557180	9	100	theme	seawater	1421:1428	arg1	fluid					1390:1394	a mineralising fluid	1375:1394	a mineralising fluid of organic compounds and seawater	1375:1428	1) A thin (< 0.5 μm) PCW forms and is filled with a mineralising fluid of organic compounds and seawater.
31557180	6	101	located	found	984:988	arg1	rhodoliths					998:1007	many rhodoliths	993:1007	many rhodoliths	993:1007	The distinctive banding found in many rhodoliths is the regular transition from PCW-only cells to SCW cells.
31557180	6	101	located	found	984:988	arg2	banding					976:982	The distinctive banding	960:982	The distinctive banding found in many rhodoliths	960:1007	The distinctive banding found in many rhodoliths is the regular transition from PCW-only cells to SCW cells.
31557180	6	101	located	found	984:988	arg2	transition					1024:1033	the regular transition	1012:1033	the regular transition from PCW-only cells to SCW cells	1012:1066	The distinctive banding found in many rhodoliths is the regular transition from PCW-only cells to SCW cells.
31557180	5	102	theme	crustose	759:766	arg1	algae					778:782	crustose coralline algae	759:782	crustose coralline algae (CCA)	759:788	We describe two types of cell wall calcification; 1) calcified primary cell wall (PCW) in the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA), 2) calcified secondary cell wall (SCW) with radial Mg-calcite crystals in thicker-walled rounded cortical cells of articulated corallines and perithallial cells of CCA.
31557180	5	102	theme	crustose	759:766	arg1	CCA					785:787	CCA	785:787	CCA	785:787	We describe two types of cell wall calcification; 1) calcified primary cell wall (PCW) in the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA), 2) calcified secondary cell wall (SCW) with radial Mg-calcite crystals in thicker-walled rounded cortical cells of articulated corallines and perithallial cells of CCA.
31557180	5	103	dep	wall	630:633	arg1	1					604:604	1	604:604	1	604:604	We describe two types of cell wall calcification; 1) calcified primary cell wall (PCW) in the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA), 2) calcified secondary cell wall (SCW) with radial Mg-calcite crystals in thicker-walled rounded cortical cells of articulated corallines and perithallial cells of CCA.
31557180	0	104	theme	process-based	51:63	arg1	understanding					65:77	A morphological and process-based understanding	31:77	A morphological and process-based understanding	31:77	Coralline algal calcification: A morphological and process-based understanding.
31557180	12	105	from	soaks	1683:1687	arg1	fluid					1709:1713	the mineralising fluid	1692:1713	the mineralising fluid	1692:1713	3) The CMF soaks in the mineralising fluid as it extrudes and becomes calcified, retaining the perpendicular form, thus building the radial calcite.
31557180	11	106	theme	synthase	1611:1618	arg1	CSC					1631:1633	CSC	1631:1633	CSC	1631:1633	2) Crystalline cellulose microfibrils (CMF) are extruded perpendicularly from the cellulose synthase complexes (CSC) in the plasmalemma to form the SCW.
31557180	11	106	theme	synthase	1611:1618	arg1	complexes					1620:1628	the cellulose synthase complexes	1597:1628	the cellulose synthase complexes (CSC)	1597:1634	2) Crystalline cellulose microfibrils (CMF) are extruded perpendicularly from the cellulose synthase complexes (CSC) in the plasmalemma to form the SCW.
31557180	5	107	theme	CCA	955:957	arg1	cells					897:901	thicker-walled rounded cortical cells	865:901	thicker-walled rounded cortical cells of articulated corallines	865:927	We describe two types of cell wall calcification; 1) calcified primary cell wall (PCW) in the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA), 2) calcified secondary cell wall (SCW) with radial Mg-calcite crystals in thicker-walled rounded cortical cells of articulated corallines and perithallial cells of CCA.
31557180	5	107	theme	CCA	955:957	arg1	cells					946:950	perithallial cells	933:950	perithallial cells of CCA	933:957	We describe two types of cell wall calcification; 1) calcified primary cell wall (PCW) in the thin-walled elongate cells such as central medullary cells in articulated corallines and hypothallial cells in crustose coralline algae (CCA), 2) calcified secondary cell wall (SCW) with radial Mg-calcite crystals in thicker-walled rounded cortical cells of articulated corallines and perithallial cells of CCA.
31557180	12	108	theme	CMF	1679:1681	arg1	soaks					1683:1687	The CMF soaks	1675:1687	The CMF soaks in the mineralising fluid	1675:1713	3) The CMF soaks in the mineralising fluid as it extrudes and becomes calcified, retaining the perpendicular form, thus building the radial calcite.
31557180	2	109	theme	thallus	367:373	arg1	formation					342:350	the formation	338:350	the formation of a calcified thallus	338:373	Their capacity to build enduring crusts that underpin the formation of tropical reefs, rhodolith beds and other benthic substrate is dependent on the formation of a calcified thallus.
31557180	7	110	theme	Mg-calcite	1174:1183	arg1	content					1133:1139	Mg content	1130:1139	Mg content of a few mol% higher than radial Mg-calcite (M-type), ranging up to dolomite composition (D-type)	1130:1237	Within the cell walls there can be bands of elevated Mg with Mg content of a few mol% higher than radial Mg-calcite (M-type), ranging up to dolomite composition (D-type).
31557180	11	111	theme	Crystalline	1522:1532	arg1	CMF					1558:1560	CMF	1558:1560	CMF	1558:1560	2) Crystalline cellulose microfibrils (CMF) are extruded perpendicularly from the cellulose synthase complexes (CSC) in the plasmalemma to form the SCW.
31557180	11	111	theme	Crystalline	1522:1532	arg1	microfibrils					1544:1555	2) Crystalline cellulose microfibrils	1519:1555	2) Crystalline cellulose microfibrils (CMF)	1519:1561	2) Crystalline cellulose microfibrils (CMF) are extruded perpendicularly from the cellulose synthase complexes (CSC) in the plasmalemma to form the SCW.
31557180	7	112	theme	Mg	1122:1123	arg1	bands					1104:1108	bands	1104:1108	bands of elevated Mg with Mg content of a few mol% higher than radial Mg-calcite (M-type), ranging up to dolomite composition (D-type)	1104:1237	Within the cell walls there can be bands of elevated Mg with Mg content of a few mol% higher than radial Mg-calcite (M-type), ranging up to dolomite composition (D-type).
31351732	12	0	theme	NaCl	2277:2280	arg1	content					2282:2288	high milk NaCl content	2267:2288	high milk NaCl content	2267:2288	In addition, high milk NaCl content led to reductions in fresh cheese yield and cheese solids.
31351732	3	1	from	yields	954:959	arg1	recovery					875:882	the recovery	871:882	the recovery of nutrients in the curd (%REC)	871:914	The aims of this study were to quantify, using milk samples from 560 dairy goats, the contemporary effects of a pool of udder health indirect indicators (lactose level, pH, SCC, TBC, and NaCl content) on the recovery of nutrients in the curd (%REC), cheese yield (%CY), and daily cheese yields (dCY).
31351732	3	2	theme	health	793:798	arg1	indicators					809:818	udder health indirect indicators	787:818	udder health indirect indicators (lactose level, pH, SCC, TBC, and NaCl content)	787:866	The aims of this study were to quantify, using milk samples from 560 dairy goats, the contemporary effects of a pool of udder health indirect indicators (lactose level, pH, SCC, TBC, and NaCl content) on the recovery of nutrients in the curd (%REC), cheese yield (%CY), and daily cheese yields (dCY).
31351732	4	3	theme	glass	1138:1142	arg1	tube					1144:1147	glass tube	1138:1147	glass tube	1138:1147	Cheese-making traits were analyzed using a mixed model, with parity, days in milk (DIM), lactose level, pH, SCC, TBC, and NaCl content as fixed effects, and farm, breed, glass tube, and animal as random effects.
31351732	1	4	theme	udder	207:211	arg1	SCC					241:243	SCC	241:243	SCC	241:243	In dairy goats, very little is known about the effect of the 2 most important indirect indicators of udder health [somatic cell count (SCC) and total bacterial count (TBC)] on milk composition and cheese yield, and no information is available regarding the effects of lactose levels, pH, and NaCl content on the recovery of nutrients in the curd, cheese yield traits, and daily cheese yields.
31351732	1	4	theme	udder	207:211	arg1	count					234:238	udder health [somatic cell count	207:238	udder health [somatic cell count (SCC)	207:244	In dairy goats, very little is known about the effect of the 2 most important indirect indicators of udder health [somatic cell count (SCC) and total bacterial count (TBC)] on milk composition and cheese yield, and no information is available regarding the effects of lactose levels, pH, and NaCl content on the recovery of nutrients in the curd, cheese yield traits, and daily cheese yields.
31351732	3	5	theme	%	931:931	arg1	yield					924:928	cheese yield	917:928	cheese yield (%CY)	917:934	The aims of this study were to quantify, using milk samples from 560 dairy goats, the contemporary effects of a pool of udder health indirect indicators (lactose level, pH, SCC, TBC, and NaCl content) on the recovery of nutrients in the curd (%REC), cheese yield (%CY), and daily cheese yields (dCY).
31351732	3	5	theme	%	931:931	arg1	CY					932:933	%CY	931:933	%CY	931:933	The aims of this study were to quantify, using milk samples from 560 dairy goats, the contemporary effects of a pool of udder health indirect indicators (lactose level, pH, SCC, TBC, and NaCl content) on the recovery of nutrients in the curd (%REC), cheese yield (%CY), and daily cheese yields (dCY).
31351732	6	6	theme	Higher	1398:1403	arg1	pH					1405:1406	Higher pH	1398:1406	Higher pH	1398:1406	Higher pH correlated with higher recovery of nutrients in the curd and higher cheese yield traits.
31351732	5	7	theme	rich	1381:1384	arg1	samples					1373:1379	samples	1373:1379	samples rich in lactose	1373:1395	Results indicated that high levels of milk lactose were associated with reduced total solids recovery in the curd and lower cheese yields, because of the lower milk fat and protein contents in samples rich in lactose.
31351732	1	8	theme	[somatic	220:227	arg1	SCC					241:243	SCC	241:243	SCC	241:243	In dairy goats, very little is known about the effect of the 2 most important indirect indicators of udder health [somatic cell count (SCC) and total bacterial count (TBC)] on milk composition and cheese yield, and no information is available regarding the effects of lactose levels, pH, and NaCl content on the recovery of nutrients in the curd, cheese yield traits, and daily cheese yields.
31351732	1	8	theme	[somatic	220:227	arg1	count					234:238	udder health [somatic cell count	207:238	udder health [somatic cell count (SCC)	207:244	In dairy goats, very little is known about the effect of the 2 most important indirect indicators of udder health [somatic cell count (SCC) and total bacterial count (TBC)] on milk composition and cheese yield, and no information is available regarding the effects of lactose levels, pH, and NaCl content on the recovery of nutrients in the curd, cheese yield traits, and daily cheese yields.
31351732	3	9	theme	lactose	821:827	arg1	level					829:833	lactose level	821:833	lactose level	821:833	The aims of this study were to quantify, using milk samples from 560 dairy goats, the contemporary effects of a pool of udder health indirect indicators (lactose level, pH, SCC, TBC, and NaCl content) on the recovery of nutrients in the curd (%REC), cheese yield (%CY), and daily cheese yields (dCY).
31351732	8	10	theme	protein	1716:1722	arg1	recovery					1704:1711	the curd but lower recovery	1685:1711	the curd but lower recovery of protein	1685:1722	High SCC were associated with higher recovery of solids and energy in the curd but lower recovery of protein.
31351732	6	11	theme	yield	1483:1487	arg1	traits					1489:1494	the curd and higher cheese yield traits	1456:1494	the curd and higher cheese yield traits	1456:1494	Higher pH correlated with higher recovery of nutrients in the curd and higher cheese yield traits.
31351732	7	12	theme	milk	1571:1574	arg1	fat					1576:1578	milk fat	1571:1578	milk fat	1571:1578	These results may be explained by the positive correlation between pH and milk fat, protein, and casein in goat milk.
31351732	7	13	from	fat	1576:1578	arg1	milk					1609:1612	goat milk	1604:1612	goat milk	1604:1612	These results may be explained by the positive correlation between pH and milk fat, protein, and casein in goat milk.
31351732	5	14	from	fat	1345:1347	arg1	samples					1373:1379	samples	1373:1379	samples rich in lactose	1373:1395	Results indicated that high levels of milk lactose were associated with reduced total solids recovery in the curd and lower cheese yields, because of the lower milk fat and protein contents in samples rich in lactose.
31351732	6	15	theme	higher	1469:1474	arg1	yield					1483:1487	higher cheese yield	1469:1487	higher cheese yield	1469:1487	Higher pH correlated with higher recovery of nutrients in the curd and higher cheese yield traits.
31351732	4	16	theme	lactose	1057:1063	arg1	level					1065:1069	lactose level	1057:1069	lactose level	1057:1069	Cheese-making traits were analyzed using a mixed model, with parity, days in milk (DIM), lactose level, pH, SCC, TBC, and NaCl content as fixed effects, and farm, breed, glass tube, and animal as random effects.
31351732	1	17	theme	levels	382:387	arg1	yields					491:496	daily cheese yields	478:496	daily cheese yields	478:496	In dairy goats, very little is known about the effect of the 2 most important indirect indicators of udder health [somatic cell count (SCC) and total bacterial count (TBC)] on milk composition and cheese yield, and no information is available regarding the effects of lactose levels, pH, and NaCl content on the recovery of nutrients in the curd, cheese yield traits, and daily cheese yields.
31351732	1	17	theme	levels	382:387	arg1	traits					466:471	cheese yield traits	453:471	cheese yield traits	453:471	In dairy goats, very little is known about the effect of the 2 most important indirect indicators of udder health [somatic cell count (SCC) and total bacterial count (TBC)] on milk composition and cheese yield, and no information is available regarding the effects of lactose levels, pH, and NaCl content on the recovery of nutrients in the curd, cheese yield traits, and daily cheese yields.
31351732	1	17	theme	levels	382:387	arg1	effects					363:369	the effects	359:369	the effects of lactose levels, pH, and NaCl content on the recovery of nutrients in the curd	359:450	In dairy goats, very little is known about the effect of the 2 most important indirect indicators of udder health [somatic cell count (SCC) and total bacterial count (TBC)] on milk composition and cheese yield, and no information is available regarding the effects of lactose levels, pH, and NaCl content on the recovery of nutrients in the curd, cheese yield traits, and daily cheese yields.
31351732	4	18	theme	random	1164:1169	arg1	effects					1171:1177	random effects	1164:1177	random effects	1164:1177	Cheese-making traits were analyzed using a mixed model, with parity, days in milk (DIM), lactose level, pH, SCC, TBC, and NaCl content as fixed effects, and farm, breed, glass tube, and animal as random effects.
31351732	4	18	theme	random	1164:1169	arg1	model					1017:1021	a mixed model	1009:1021	a mixed model	1009:1021	Cheese-making traits were analyzed using a mixed model, with parity, days in milk (DIM), lactose level, pH, SCC, TBC, and NaCl content as fixed effects, and farm, breed, glass tube, and animal as random effects.
31351732	1	19	from	traits	466:471	arg1	recovery					418:425	the recovery	414:425	the recovery of nutrients in the curd	414:450	In dairy goats, very little is known about the effect of the 2 most important indirect indicators of udder health [somatic cell count (SCC) and total bacterial count (TBC)] on milk composition and cheese yield, and no information is available regarding the effects of lactose levels, pH, and NaCl content on the recovery of nutrients in the curd, cheese yield traits, and daily cheese yields.
31351732	6	20	theme	curd	1460:1463	arg1	traits					1489:1494	the curd and higher cheese yield traits	1456:1494	the curd and higher cheese yield traits	1456:1494	Higher pH correlated with higher recovery of nutrients in the curd and higher cheese yield traits.
31351732	5	21	theme	total	1260:1264	arg1	solids					1266:1271	total solids	1260:1271	reduced total solids recovery in the curd and lower cheese yields	1252:1316	Results indicated that high levels of milk lactose were associated with reduced total solids recovery in the curd and lower cheese yields, because of the lower milk fat and protein contents in samples rich in lactose.
31351732	9	22	from	recovery	1808:1815	arg1	curd					1835:1838	the curd	1831:1838	the curd	1831:1838	The higher cheese yield obtained from milk with high SCC was due to both increased recovery of lactose in the curd and water retention.
31351732	9	23	theme	increased	1798:1806	arg1	recovery					1808:1815	increased recovery	1798:1815	increased recovery of lactose in the curd	1798:1838	The higher cheese yield obtained from milk with high SCC was due to both increased recovery of lactose in the curd and water retention.
31351732	5	24	theme	milk	1218:1221	arg1	lactose					1223:1229	milk lactose	1218:1229	milk lactose	1218:1229	Results indicated that high levels of milk lactose were associated with reduced total solids recovery in the curd and lower cheese yields, because of the lower milk fat and protein contents in samples rich in lactose.
31351732	3	25	theme	cheese	947:952	arg1	yields					954:959	daily cheese yields	941:959	daily cheese yields (dCY)	941:965	The aims of this study were to quantify, using milk samples from 560 dairy goats, the contemporary effects of a pool of udder health indirect indicators (lactose level, pH, SCC, TBC, and NaCl content) on the recovery of nutrients in the curd (%REC), cheese yield (%CY), and daily cheese yields (dCY).
31351732	3	25	theme	cheese	947:952	arg1	dCY					962:964	dCY	962:964	dCY	962:964	The aims of this study were to quantify, using milk samples from 560 dairy goats, the contemporary effects of a pool of udder health indirect indicators (lactose level, pH, SCC, TBC, and NaCl content) on the recovery of nutrients in the curd (%REC), cheese yield (%CY), and daily cheese yields (dCY).
31351732	5	26	dep	curd	1289:1292	arg1	the					1285:1287	the	1285:1287	the	1285:1287	Results indicated that high levels of milk lactose were associated with reduced total solids recovery in the curd and lower cheese yields, because of the lower milk fat and protein contents in samples rich in lactose.
31351732	9	27	theme	higher	1729:1734	arg1	yield					1743:1747	The higher cheese yield	1725:1747	The higher cheese yield obtained from milk with high SCC	1725:1780	The higher cheese yield obtained from milk with high SCC was due to both increased recovery of lactose in the curd and water retention.
31351732	9	27	theme	higher	1729:1734	arg1	due					1786:1788	due	1786:1788	due	1786:1788	The higher cheese yield obtained from milk with high SCC was due to both increased recovery of lactose in the curd and water retention.
31351732	1	28	from	effect	153:158	arg1	yield					310:314	cheese yield	303:314	cheese yield	303:314	In dairy goats, very little is known about the effect of the 2 most important indirect indicators of udder health [somatic cell count (SCC) and total bacterial count (TBC)] on milk composition and cheese yield, and no information is available regarding the effects of lactose levels, pH, and NaCl content on the recovery of nutrients in the curd, cheese yield traits, and daily cheese yields.
31351732	1	28	from	effect	153:158	arg1	composition					287:297	milk composition	282:297	milk composition	282:297	In dairy goats, very little is known about the effect of the 2 most important indirect indicators of udder health [somatic cell count (SCC) and total bacterial count (TBC)] on milk composition and cheese yield, and no information is available regarding the effects of lactose levels, pH, and NaCl content on the recovery of nutrients in the curd, cheese yield traits, and daily cheese yields.
31351732	5	29	from	contents	1361:1368	arg1	samples					1373:1379	samples	1373:1379	samples rich in lactose	1373:1395	Results indicated that high levels of milk lactose were associated with reduced total solids recovery in the curd and lower cheese yields, because of the lower milk fat and protein contents in samples rich in lactose.
31351732	10	30	theme	legal	2022:2026	arg1	limits					2028:2033	the legal limits	2018:2033	the legal limits	2018:2033	Bacterial count proved to be the least important factor affecting cheese-making traits, but it decreased daily cheese yields, suggesting that, even if below the legal limits, TBC should be considered in order to monitor flock management and avoid economic losses.
31351732	4	31	from	animal	1154:1159	arg1	DIM					1051:1053	DIM	1051:1053	DIM	1051:1053	Cheese-making traits were analyzed using a mixed model, with parity, days in milk (DIM), lactose level, pH, SCC, TBC, and NaCl content as fixed effects, and farm, breed, glass tube, and animal as random effects.
31351732	4	31	from	animal	1154:1159	arg1	farm					1125:1128	farm	1125:1128	farm	1125:1128	Cheese-making traits were analyzed using a mixed model, with parity, days in milk (DIM), lactose level, pH, SCC, TBC, and NaCl content as fixed effects, and farm, breed, glass tube, and animal as random effects.
31351732	4	31	from	animal	1154:1159	arg1	milk					1045:1048	milk	1045:1048	milk (DIM)	1045:1054	Cheese-making traits were analyzed using a mixed model, with parity, days in milk (DIM), lactose level, pH, SCC, TBC, and NaCl content as fixed effects, and farm, breed, glass tube, and animal as random effects.
31351732	4	31	from	animal	1154:1159	arg1	level					1065:1069	lactose level	1057:1069	lactose level	1057:1069	Cheese-making traits were analyzed using a mixed model, with parity, days in milk (DIM), lactose level, pH, SCC, TBC, and NaCl content as fixed effects, and farm, breed, glass tube, and animal as random effects.
31351732	1	32	theme	dairy	109:113	arg1	goats					115:119	dairy goats	109:119	dairy goats	109:119	In dairy goats, very little is known about the effect of the 2 most important indirect indicators of udder health [somatic cell count (SCC) and total bacterial count (TBC)] on milk composition and cheese yield, and no information is available regarding the effects of lactose levels, pH, and NaCl content on the recovery of nutrients in the curd, cheese yield traits, and daily cheese yields.
31351732	3	33	theme	study	684:688	arg1	aims					671:674	The aims	667:674	The aims of this study	667:688	The aims of this study were to quantify, using milk samples from 560 dairy goats, the contemporary effects of a pool of udder health indirect indicators (lactose level, pH, SCC, TBC, and NaCl content) on the recovery of nutrients in the curd (%REC), cheese yield (%CY), and daily cheese yields (dCY).
31351732	12	34	theme	cheese	2317:2322	arg1	yield					2324:2328	fresh cheese yield	2311:2328	fresh cheese yield	2311:2328	In addition, high milk NaCl content led to reductions in fresh cheese yield and cheese solids.
31351732	11	35	theme	lower	2188:2192	arg1	recovery					2194:2201	lower recovery	2188:2201	lower recovery of all nutrients in the curd during cheese-making	2188:2251	The effect of NaCl content on milk composition was linked with lower recovery of all nutrients in the curd during cheese-making.
31351732	1	36	theme	daily	478:482	arg1	yields					491:496	daily cheese yields	478:496	daily cheese yields	478:496	In dairy goats, very little is known about the effect of the 2 most important indirect indicators of udder health [somatic cell count (SCC) and total bacterial count (TBC)] on milk composition and cheese yield, and no information is available regarding the effects of lactose levels, pH, and NaCl content on the recovery of nutrients in the curd, cheese yield traits, and daily cheese yields.
31351732	4	37	theme	NaCl	1090:1093	arg1	content					1095:1101	NaCl content	1090:1101	NaCl content	1090:1101	Cheese-making traits were analyzed using a mixed model, with parity, days in milk (DIM), lactose level, pH, SCC, TBC, and NaCl content as fixed effects, and farm, breed, glass tube, and animal as random effects.
31351732	0	38	theme	nutrient	50:57	arg1	recovery					59:66	nutrient recovery	50:66	nutrient recovery	50:66	Effects of indirect indicators of udder health on nutrient recovery and cheese yield traits in goat milk.
31351732	1	39	theme	count	266:270	arg1	indicators					193:202	the 2 most important indirect indicators	163:202	the 2 most important indirect indicators of udder health [somatic cell count (SCC) and total bacterial count (TBC)]	163:277	In dairy goats, very little is known about the effect of the 2 most important indirect indicators of udder health [somatic cell count (SCC) and total bacterial count (TBC)] on milk composition and cheese yield, and no information is available regarding the effects of lactose levels, pH, and NaCl content on the recovery of nutrients in the curd, cheese yield traits, and daily cheese yields.
31351732	10	40	theme	cheese-making	1927:1939	arg1	traits					1941:1946	cheese-making traits	1927:1946	cheese-making traits	1927:1946	Bacterial count proved to be the least important factor affecting cheese-making traits, but it decreased daily cheese yields, suggesting that, even if below the legal limits, TBC should be considered in order to monitor flock management and avoid economic losses.
31351732	3	41	theme	milk	714:717	arg1	samples					719:725	milk samples	714:725	milk samples from 560 dairy goats	714:746	The aims of this study were to quantify, using milk samples from 560 dairy goats, the contemporary effects of a pool of udder health indirect indicators (lactose level, pH, SCC, TBC, and NaCl content) on the recovery of nutrients in the curd (%REC), cheese yield (%CY), and daily cheese yields (dCY).
31351732	4	42	with	model	1017:1021	arg1	animal					1154:1159	animal	1154:1159	animal	1154:1159	Cheese-making traits were analyzed using a mixed model, with parity, days in milk (DIM), lactose level, pH, SCC, TBC, and NaCl content as fixed effects, and farm, breed, glass tube, and animal as random effects.
31351732	4	42	with	model	1017:1021	arg1	breed					1131:1135	breed	1131:1135	breed	1131:1135	Cheese-making traits were analyzed using a mixed model, with parity, days in milk (DIM), lactose level, pH, SCC, TBC, and NaCl content as fixed effects, and farm, breed, glass tube, and animal as random effects.
31351732	4	42	with	model	1017:1021	arg1	tube					1144:1147	glass tube	1138:1147	glass tube	1138:1147	Cheese-making traits were analyzed using a mixed model, with parity, days in milk (DIM), lactose level, pH, SCC, TBC, and NaCl content as fixed effects, and farm, breed, glass tube, and animal as random effects.
31351732	4	42	with	model	1017:1021	arg1	days					1037:1040	days	1037:1040	days in milk (DIM), lactose level, pH, SCC, TBC, and NaCl content as fixed effects, and farm	1037:1128	Cheese-making traits were analyzed using a mixed model, with parity, days in milk (DIM), lactose level, pH, SCC, TBC, and NaCl content as fixed effects, and farm, breed, glass tube, and animal as random effects.
31351732	4	42	with	model	1017:1021	arg1	parity					1029:1034	parity	1029:1034	parity	1029:1034	Cheese-making traits were analyzed using a mixed model, with parity, days in milk (DIM), lactose level, pH, SCC, TBC, and NaCl content as fixed effects, and farm, breed, glass tube, and animal as random effects.
31351732	3	43	theme	NaCl	854:857	arg1	content					859:865	NaCl content	854:865	NaCl content	854:865	The aims of this study were to quantify, using milk samples from 560 dairy goats, the contemporary effects of a pool of udder health indirect indicators (lactose level, pH, SCC, TBC, and NaCl content) on the recovery of nutrients in the curd (%REC), cheese yield (%CY), and daily cheese yields (dCY).
31351732	0	44	from	Effects	0:6	arg1	cheese					72:77	cheese	72:77	cheese	72:77	Effects of indirect indicators of udder health on nutrient recovery and cheese yield traits in goat milk.
31351732	0	44	from	Effects	0:6	arg1	recovery					59:66	nutrient recovery	50:66	nutrient recovery	50:66	Effects of indirect indicators of udder health on nutrient recovery and cheese yield traits in goat milk.
31351732	4	45	from	breed	1131:1135	arg1	DIM					1051:1053	DIM	1051:1053	DIM	1051:1053	Cheese-making traits were analyzed using a mixed model, with parity, days in milk (DIM), lactose level, pH, SCC, TBC, and NaCl content as fixed effects, and farm, breed, glass tube, and animal as random effects.
31351732	4	45	from	breed	1131:1135	arg1	farm					1125:1128	farm	1125:1128	farm	1125:1128	Cheese-making traits were analyzed using a mixed model, with parity, days in milk (DIM), lactose level, pH, SCC, TBC, and NaCl content as fixed effects, and farm, breed, glass tube, and animal as random effects.
31351732	4	45	from	breed	1131:1135	arg1	milk					1045:1048	milk	1045:1048	milk (DIM)	1045:1054	Cheese-making traits were analyzed using a mixed model, with parity, days in milk (DIM), lactose level, pH, SCC, TBC, and NaCl content as fixed effects, and farm, breed, glass tube, and animal as random effects.
31351732	4	45	from	breed	1131:1135	arg1	level					1065:1069	lactose level	1057:1069	lactose level	1057:1069	Cheese-making traits were analyzed using a mixed model, with parity, days in milk (DIM), lactose level, pH, SCC, TBC, and NaCl content as fixed effects, and farm, breed, glass tube, and animal as random effects.
31351732	14	46	theme	economic	2512:2519	arg1	value					2521:2525	the milk-to-cheese economic value	2493:2525	the milk-to-cheese economic value	2493:2525	Such information should be considered, to adjust the milk-to-cheese economic value and the milk payment system.
31351732	9	47	from	retention	1850:1858	arg1	curd					1835:1838	the curd	1831:1838	the curd	1831:1838	The higher cheese yield obtained from milk with high SCC was due to both increased recovery of lactose in the curd and water retention.
31351732	3	48	theme	dairy	736:740	arg1	goats					742:746	560 dairy goats	732:746	560 dairy goats	732:746	The aims of this study were to quantify, using milk samples from 560 dairy goats, the contemporary effects of a pool of udder health indirect indicators (lactose level, pH, SCC, TBC, and NaCl content) on the recovery of nutrients in the curd (%REC), cheese yield (%CY), and daily cheese yields (dCY).
31351732	10	49	theme	important	1900:1908	arg1	factor					1910:1915	the least important factor	1890:1915	the least important factor affecting cheese-making traits	1890:1946	Bacterial count proved to be the least important factor affecting cheese-making traits, but it decreased daily cheese yields, suggesting that, even if below the legal limits, TBC should be considered in order to monitor flock management and avoid economic losses.
31351732	10	49	theme	important	1900:1908	arg1	count					1871:1875	Bacterial count	1861:1875	Bacterial count	1861:1875	Bacterial count proved to be the least important factor affecting cheese-making traits, but it decreased daily cheese yields, suggesting that, even if below the legal limits, TBC should be considered in order to monitor flock management and avoid economic losses.
31351732	2	50	theme	large	507:511	arg1	differences					513:523	large differences	507:523	large differences	507:523	Because large differences exist among dairy species, conclusions from the most studied species (i.e., bovine) cannot be drawn for all types of dairy-producing animals.
31351732	1	51	theme	nutrients	430:438	arg1	recovery					418:425	the recovery	414:425	the recovery of nutrients in the curd	414:450	In dairy goats, very little is known about the effect of the 2 most important indirect indicators of udder health [somatic cell count (SCC) and total bacterial count (TBC)] on milk composition and cheese yield, and no information is available regarding the effects of lactose levels, pH, and NaCl content on the recovery of nutrients in the curd, cheese yield traits, and daily cheese yields.
31351732	1	52	theme	milk	282:285	arg1	composition					287:297	milk composition	282:297	milk composition	282:297	In dairy goats, very little is known about the effect of the 2 most important indirect indicators of udder health [somatic cell count (SCC) and total bacterial count (TBC)] on milk composition and cheese yield, and no information is available regarding the effects of lactose levels, pH, and NaCl content on the recovery of nutrients in the curd, cheese yield traits, and daily cheese yields.
31351732	1	53	from	recovery	418:425	arg1	curd					447:450	the curd	443:450	the curd	443:450	In dairy goats, very little is known about the effect of the 2 most important indirect indicators of udder health [somatic cell count (SCC) and total bacterial count (TBC)] on milk composition and cheese yield, and no information is available regarding the effects of lactose levels, pH, and NaCl content on the recovery of nutrients in the curd, cheese yield traits, and daily cheese yields.
31351732	12	54	theme	milk	2272:2275	arg1	content					2282:2288	high milk NaCl content	2267:2288	high milk NaCl content	2267:2288	In addition, high milk NaCl content led to reductions in fresh cheese yield and cheese solids.
31351732	5	55	theme	lower	1298:1302	arg1	yields					1311:1316	lower cheese yields	1298:1316	lower cheese yields	1298:1316	Results indicated that high levels of milk lactose were associated with reduced total solids recovery in the curd and lower cheese yields, because of the lower milk fat and protein contents in samples rich in lactose.
31351732	1	56	theme	cell	229:232	arg1	SCC					241:243	SCC	241:243	SCC	241:243	In dairy goats, very little is known about the effect of the 2 most important indirect indicators of udder health [somatic cell count (SCC) and total bacterial count (TBC)] on milk composition and cheese yield, and no information is available regarding the effects of lactose levels, pH, and NaCl content on the recovery of nutrients in the curd, cheese yield traits, and daily cheese yields.
31351732	1	56	theme	cell	229:232	arg1	count					234:238	udder health [somatic cell count	207:238	udder health [somatic cell count (SCC)	207:244	In dairy goats, very little is known about the effect of the 2 most important indirect indicators of udder health [somatic cell count (SCC) and total bacterial count (TBC)] on milk composition and cheese yield, and no information is available regarding the effects of lactose levels, pH, and NaCl content on the recovery of nutrients in the curd, cheese yield traits, and daily cheese yields.
31351732	3	57	theme	contemporary	753:764	arg1	effects					766:772	the contemporary effects	749:772	the contemporary effects of a pool of udder health indirect indicators (lactose level, pH, SCC, TBC, and NaCl content) on the recovery of nutrients in the curd (%REC)	749:914	The aims of this study were to quantify, using milk samples from 560 dairy goats, the contemporary effects of a pool of udder health indirect indicators (lactose level, pH, SCC, TBC, and NaCl content) on the recovery of nutrients in the curd (%REC), cheese yield (%CY), and daily cheese yields (dCY).
31351732	13	58	theme	cheese-making	2421:2433	arg1	process					2435:2441	the cheese-making process	2417:2441	the cheese-making process	2417:2441	The indirect indicators of the present study significantly affected the cheese-making process.
31351732	2	59	theme	dairy	537:541	arg1	species					543:549	dairy species	537:549	dairy species	537:549	Because large differences exist among dairy species, conclusions from the most studied species (i.e., bovine) cannot be drawn for all types of dairy-producing animals.
31351732	5	60	theme	solids	1266:1271	arg1	recovery					1273:1280	reduced total solids recovery	1252:1280	reduced total solids recovery in the curd and lower cheese yields	1252:1316	Results indicated that high levels of milk lactose were associated with reduced total solids recovery in the curd and lower cheese yields, because of the lower milk fat and protein contents in samples rich in lactose.
31351732	0	61	theme	indirect	11:18	arg1	indicators					20:29	indirect indicators	11:29	indirect indicators of udder health	11:45	Effects of indirect indicators of udder health on nutrient recovery and cheese yield traits in goat milk.
31351732	3	62	from	yield	924:928	arg1	recovery					875:882	the recovery	871:882	the recovery of nutrients in the curd (%REC)	871:914	The aims of this study were to quantify, using milk samples from 560 dairy goats, the contemporary effects of a pool of udder health indirect indicators (lactose level, pH, SCC, TBC, and NaCl content) on the recovery of nutrients in the curd (%REC), cheese yield (%CY), and daily cheese yields (dCY).
31351732	10	63	theme	cheese	1972:1977	arg1	yields					1979:1984	cheese yields	1972:1984	cheese yields	1972:1984	Bacterial count proved to be the least important factor affecting cheese-making traits, but it decreased daily cheese yields, suggesting that, even if below the legal limits, TBC should be considered in order to monitor flock management and avoid economic losses.
31351732	1	64	theme	yield	460:464	arg1	traits					466:471	cheese yield traits	453:471	cheese yield traits	453:471	In dairy goats, very little is known about the effect of the 2 most important indirect indicators of udder health [somatic cell count (SCC) and total bacterial count (TBC)] on milk composition and cheese yield, and no information is available regarding the effects of lactose levels, pH, and NaCl content on the recovery of nutrients in the curd, cheese yield traits, and daily cheese yields.
31351732	9	65	theme	lactose	1820:1826	arg1	retention					1850:1858	water retention	1844:1858	water retention	1844:1858	The higher cheese yield obtained from milk with high SCC was due to both increased recovery of lactose in the curd and water retention.
31351732	9	65	theme	lactose	1820:1826	arg1	recovery					1808:1815	increased recovery	1798:1815	increased recovery of lactose in the curd	1798:1838	The higher cheese yield obtained from milk with high SCC was due to both increased recovery of lactose in the curd and water retention.
31351732	11	66	theme	nutrients	2210:2218	arg1	recovery					2194:2201	lower recovery	2188:2201	lower recovery of all nutrients in the curd during cheese-making	2188:2251	The effect of NaCl content on milk composition was linked with lower recovery of all nutrients in the curd during cheese-making.
31351732	3	67	theme	pool	779:782	arg1	yield					924:928	cheese yield	917:928	cheese yield (%CY)	917:934	The aims of this study were to quantify, using milk samples from 560 dairy goats, the contemporary effects of a pool of udder health indirect indicators (lactose level, pH, SCC, TBC, and NaCl content) on the recovery of nutrients in the curd (%REC), cheese yield (%CY), and daily cheese yields (dCY).
31351732	3	67	theme	pool	779:782	arg1	effects					766:772	the contemporary effects	749:772	the contemporary effects of a pool of udder health indirect indicators (lactose level, pH, SCC, TBC, and NaCl content) on the recovery of nutrients in the curd (%REC)	749:914	The aims of this study were to quantify, using milk samples from 560 dairy goats, the contemporary effects of a pool of udder health indirect indicators (lactose level, pH, SCC, TBC, and NaCl content) on the recovery of nutrients in the curd (%REC), cheese yield (%CY), and daily cheese yields (dCY).
31351732	3	67	theme	pool	779:782	arg1	CY					932:933	%CY	931:933	%CY	931:933	The aims of this study were to quantify, using milk samples from 560 dairy goats, the contemporary effects of a pool of udder health indirect indicators (lactose level, pH, SCC, TBC, and NaCl content) on the recovery of nutrients in the curd (%REC), cheese yield (%CY), and daily cheese yields (dCY).
31351732	3	67	theme	pool	779:782	arg1	yields					954:959	daily cheese yields	941:959	daily cheese yields (dCY)	941:965	The aims of this study were to quantify, using milk samples from 560 dairy goats, the contemporary effects of a pool of udder health indirect indicators (lactose level, pH, SCC, TBC, and NaCl content) on the recovery of nutrients in the curd (%REC), cheese yield (%CY), and daily cheese yields (dCY).
31351732	3	67	theme	pool	779:782	arg1	dCY					962:964	dCY	962:964	dCY	962:964	The aims of this study were to quantify, using milk samples from 560 dairy goats, the contemporary effects of a pool of udder health indirect indicators (lactose level, pH, SCC, TBC, and NaCl content) on the recovery of nutrients in the curd (%REC), cheese yield (%CY), and daily cheese yields (dCY).
31351732	3	67	theme	pool	779:782	arg1	samples					719:725	milk samples	714:725	milk samples from 560 dairy goats	714:746	The aims of this study were to quantify, using milk samples from 560 dairy goats, the contemporary effects of a pool of udder health indirect indicators (lactose level, pH, SCC, TBC, and NaCl content) on the recovery of nutrients in the curd (%REC), cheese yield (%CY), and daily cheese yields (dCY).
31351732	8	68	theme	higher	1645:1650	arg1	recovery					1652:1659	higher recovery	1645:1659	higher recovery of solids and energy in the curd but lower recovery of protein	1645:1722	High SCC were associated with higher recovery of solids and energy in the curd but lower recovery of protein.
31351732	11	69	theme	content	2144:2150	arg1	effect					2129:2134	The effect	2125:2134	The effect of NaCl content on milk composition	2125:2170	The effect of NaCl content on milk composition was linked with lower recovery of all nutrients in the curd during cheese-making.
31351732	14	70	theme	milk	2535:2538	arg1	system					2548:2553	the milk payment system	2531:2553	the milk payment system	2531:2553	Such information should be considered, to adjust the milk-to-cheese economic value and the milk payment system.
31351732	0	71	theme	health	40:45	arg1	indicators					20:29	indirect indicators	11:29	indirect indicators of udder health	11:45	Effects of indirect indicators of udder health on nutrient recovery and cheese yield traits in goat milk.
31351732	1	72	theme	total	250:254	arg1	TBC					273:275	TBC	273:275	TBC	273:275	In dairy goats, very little is known about the effect of the 2 most important indirect indicators of udder health [somatic cell count (SCC) and total bacterial count (TBC)] on milk composition and cheese yield, and no information is available regarding the effects of lactose levels, pH, and NaCl content on the recovery of nutrients in the curd, cheese yield traits, and daily cheese yields.
31351732	1	72	theme	total	250:254	arg1	count					266:270	total bacterial count	250:270	total bacterial count (TBC)]	250:277	In dairy goats, very little is known about the effect of the 2 most important indirect indicators of udder health [somatic cell count (SCC) and total bacterial count (TBC)] on milk composition and cheese yield, and no information is available regarding the effects of lactose levels, pH, and NaCl content on the recovery of nutrients in the curd, cheese yield traits, and daily cheese yields.
31351732	3	73	theme	%	910:910	arg1	curd					904:907	the curd	900:907	the curd (%REC)	900:914	The aims of this study were to quantify, using milk samples from 560 dairy goats, the contemporary effects of a pool of udder health indirect indicators (lactose level, pH, SCC, TBC, and NaCl content) on the recovery of nutrients in the curd (%REC), cheese yield (%CY), and daily cheese yields (dCY).
31351732	3	73	theme	%	910:910	arg1	REC					911:913	%REC	910:913	%REC	910:913	The aims of this study were to quantify, using milk samples from 560 dairy goats, the contemporary effects of a pool of udder health indirect indicators (lactose level, pH, SCC, TBC, and NaCl content) on the recovery of nutrients in the curd (%REC), cheese yield (%CY), and daily cheese yields (dCY).
31351732	7	74	from	casein	1594:1599	arg1	milk					1609:1612	goat milk	1604:1612	goat milk	1604:1612	These results may be explained by the positive correlation between pH and milk fat, protein, and casein in goat milk.
31351732	1	75	theme	NaCl	398:401	arg1	content					403:409	NaCl content	398:409	NaCl content	398:409	In dairy goats, very little is known about the effect of the 2 most important indirect indicators of udder health [somatic cell count (SCC) and total bacterial count (TBC)] on milk composition and cheese yield, and no information is available regarding the effects of lactose levels, pH, and NaCl content on the recovery of nutrients in the curd, cheese yield traits, and daily cheese yields.
31351732	5	76	theme	milk	1340:1343	arg1	fat					1345:1347	the lower milk fat	1330:1347	the lower milk fat	1330:1347	Results indicated that high levels of milk lactose were associated with reduced total solids recovery in the curd and lower cheese yields, because of the lower milk fat and protein contents in samples rich in lactose.
31351732	3	77	from	samples	719:725	arg1	recovery					875:882	the recovery	871:882	the recovery of nutrients in the curd (%REC)	871:914	The aims of this study were to quantify, using milk samples from 560 dairy goats, the contemporary effects of a pool of udder health indirect indicators (lactose level, pH, SCC, TBC, and NaCl content) on the recovery of nutrients in the curd (%REC), cheese yield (%CY), and daily cheese yields (dCY).
31351732	3	78	theme	indirect	800:807	arg1	indicators					809:818	udder health indirect indicators	787:818	udder health indirect indicators (lactose level, pH, SCC, TBC, and NaCl content)	787:866	The aims of this study were to quantify, using milk samples from 560 dairy goats, the contemporary effects of a pool of udder health indirect indicators (lactose level, pH, SCC, TBC, and NaCl content) on the recovery of nutrients in the curd (%REC), cheese yield (%CY), and daily cheese yields (dCY).
31351732	2	79	theme	dairy-producing	642:656	arg1	animals					658:664	dairy-producing animals	642:664	dairy-producing animals	642:664	Because large differences exist among dairy species, conclusions from the most studied species (i.e., bovine) cannot be drawn for all types of dairy-producing animals.
31351732	4	80	theme	Cheese-making	968:980	arg1	traits					982:987	Cheese-making traits	968:987	Cheese-making traits	968:987	Cheese-making traits were analyzed using a mixed model, with parity, days in milk (DIM), lactose level, pH, SCC, TBC, and NaCl content as fixed effects, and farm, breed, glass tube, and animal as random effects.
31351732	1	81	theme	cheese	484:489	arg1	yields					491:496	daily cheese yields	478:496	daily cheese yields	478:496	In dairy goats, very little is known about the effect of the 2 most important indirect indicators of udder health [somatic cell count (SCC) and total bacterial count (TBC)] on milk composition and cheese yield, and no information is available regarding the effects of lactose levels, pH, and NaCl content on the recovery of nutrients in the curd, cheese yield traits, and daily cheese yields.
31351732	1	82	theme	indirect	184:191	arg1	indicators					193:202	the 2 most important indirect indicators	163:202	the 2 most important indirect indicators of udder health [somatic cell count (SCC) and total bacterial count (TBC)]	163:277	In dairy goats, very little is known about the effect of the 2 most important indirect indicators of udder health [somatic cell count (SCC) and total bacterial count (TBC)] on milk composition and cheese yield, and no information is available regarding the effects of lactose levels, pH, and NaCl content on the recovery of nutrients in the curd, cheese yield traits, and daily cheese yields.
31351732	3	83	theme	cheese	917:922	arg1	yield					924:928	cheese yield	917:928	cheese yield (%CY)	917:934	The aims of this study were to quantify, using milk samples from 560 dairy goats, the contemporary effects of a pool of udder health indirect indicators (lactose level, pH, SCC, TBC, and NaCl content) on the recovery of nutrients in the curd (%REC), cheese yield (%CY), and daily cheese yields (dCY).
31351732	3	83	theme	cheese	917:922	arg1	CY					932:933	%CY	931:933	%CY	931:933	The aims of this study were to quantify, using milk samples from 560 dairy goats, the contemporary effects of a pool of udder health indirect indicators (lactose level, pH, SCC, TBC, and NaCl content) on the recovery of nutrients in the curd (%REC), cheese yield (%CY), and daily cheese yields (dCY).
31351732	13	84	theme	indirect	2353:2360	arg1	indicators					2362:2371	The indirect indicators	2349:2371	The indirect indicators of the present study	2349:2392	The indirect indicators of the present study significantly affected the cheese-making process.
31351732	3	85	theme	udder	787:791	arg1	indicators					809:818	udder health indirect indicators	787:818	udder health indirect indicators (lactose level, pH, SCC, TBC, and NaCl content)	787:866	The aims of this study were to quantify, using milk samples from 560 dairy goats, the contemporary effects of a pool of udder health indirect indicators (lactose level, pH, SCC, TBC, and NaCl content) on the recovery of nutrients in the curd (%REC), cheese yield (%CY), and daily cheese yields (dCY).
31351732	1	86	from	effects	363:369	arg1	recovery					418:425	the recovery	414:425	the recovery of nutrients in the curd	414:450	In dairy goats, very little is known about the effect of the 2 most important indirect indicators of udder health [somatic cell count (SCC) and total bacterial count (TBC)] on milk composition and cheese yield, and no information is available regarding the effects of lactose levels, pH, and NaCl content on the recovery of nutrients in the curd, cheese yield traits, and daily cheese yields.
31351732	5	87	theme	high	1203:1206	arg1	levels					1208:1213	high levels	1203:1213	high levels of milk lactose	1203:1229	Results indicated that high levels of milk lactose were associated with reduced total solids recovery in the curd and lower cheese yields, because of the lower milk fat and protein contents in samples rich in lactose.
31351732	8	88	theme	curd	1689:1692	arg1	recovery					1704:1711	the curd but lower recovery	1685:1711	the curd but lower recovery of protein	1685:1722	High SCC were associated with higher recovery of solids and energy in the curd but lower recovery of protein.
31351732	1	89	theme	health	213:218	arg1	SCC					241:243	SCC	241:243	SCC	241:243	In dairy goats, very little is known about the effect of the 2 most important indirect indicators of udder health [somatic cell count (SCC) and total bacterial count (TBC)] on milk composition and cheese yield, and no information is available regarding the effects of lactose levels, pH, and NaCl content on the recovery of nutrients in the curd, cheese yield traits, and daily cheese yields.
31351732	1	89	theme	health	213:218	arg1	count					234:238	udder health [somatic cell count	207:238	udder health [somatic cell count (SCC)	207:244	In dairy goats, very little is known about the effect of the 2 most important indirect indicators of udder health [somatic cell count (SCC) and total bacterial count (TBC)] on milk composition and cheese yield, and no information is available regarding the effects of lactose levels, pH, and NaCl content on the recovery of nutrients in the curd, cheese yield traits, and daily cheese yields.
31351732	7	90	from	pH	1564:1565	arg1	milk					1609:1612	goat milk	1604:1612	goat milk	1604:1612	These results may be explained by the positive correlation between pH and milk fat, protein, and casein in goat milk.
31351732	6	91	from	recovery	1431:1438	arg1	traits					1489:1494	the curd and higher cheese yield traits	1456:1494	the curd and higher cheese yield traits	1456:1494	Higher pH correlated with higher recovery of nutrients in the curd and higher cheese yield traits.
31351732	8	92	theme	lower	1698:1702	arg1	recovery					1704:1711	the curd but lower recovery	1685:1711	the curd but lower recovery of protein	1685:1722	High SCC were associated with higher recovery of solids and energy in the curd but lower recovery of protein.
31351732	4	93	theme	mixed	1011:1015	arg1	model					1017:1021	a mixed model	1009:1021	a mixed model	1009:1021	Cheese-making traits were analyzed using a mixed model, with parity, days in milk (DIM), lactose level, pH, SCC, TBC, and NaCl content as fixed effects, and farm, breed, glass tube, and animal as random effects.
31351732	4	93	theme	mixed	1011:1015	arg1	effects					1171:1177	random effects	1164:1177	random effects	1164:1177	Cheese-making traits were analyzed using a mixed model, with parity, days in milk (DIM), lactose level, pH, SCC, TBC, and NaCl content as fixed effects, and farm, breed, glass tube, and animal as random effects.
31351732	14	94	theme	Such	2444:2447	arg1	information					2449:2459	Such information	2444:2459	Such information	2444:2459	Such information should be considered, to adjust the milk-to-cheese economic value and the milk payment system.
31351732	1	95	from	yields	491:496	arg1	recovery					418:425	the recovery	414:425	the recovery of nutrients in the curd	414:450	In dairy goats, very little is known about the effect of the 2 most important indirect indicators of udder health [somatic cell count (SCC) and total bacterial count (TBC)] on milk composition and cheese yield, and no information is available regarding the effects of lactose levels, pH, and NaCl content on the recovery of nutrients in the curd, cheese yield traits, and daily cheese yields.
31351732	6	96	theme	cheese	1476:1481	arg1	yield					1483:1487	higher cheese yield	1469:1487	higher cheese yield	1469:1487	Higher pH correlated with higher recovery of nutrients in the curd and higher cheese yield traits.
31351732	1	97	theme	lactose	374:380	arg1	levels					382:387	lactose levels	374:387	lactose levels	374:387	In dairy goats, very little is known about the effect of the 2 most important indirect indicators of udder health [somatic cell count (SCC) and total bacterial count (TBC)] on milk composition and cheese yield, and no information is available regarding the effects of lactose levels, pH, and NaCl content on the recovery of nutrients in the curd, cheese yield traits, and daily cheese yields.
31351732	3	98	theme	daily	941:945	arg1	yields					954:959	daily cheese yields	941:959	daily cheese yields (dCY)	941:965	The aims of this study were to quantify, using milk samples from 560 dairy goats, the contemporary effects of a pool of udder health indirect indicators (lactose level, pH, SCC, TBC, and NaCl content) on the recovery of nutrients in the curd (%REC), cheese yield (%CY), and daily cheese yields (dCY).
31351732	3	98	theme	daily	941:945	arg1	dCY					962:964	dCY	962:964	dCY	962:964	The aims of this study were to quantify, using milk samples from 560 dairy goats, the contemporary effects of a pool of udder health indirect indicators (lactose level, pH, SCC, TBC, and NaCl content) on the recovery of nutrients in the curd (%REC), cheese yield (%CY), and daily cheese yields (dCY).
31351732	13	99	theme	present	2380:2386	arg1	study					2388:2392	the present study	2376:2392	the present study	2376:2392	The indirect indicators of the present study significantly affected the cheese-making process.
31351732	5	100	theme	reduced	1252:1258	arg1	recovery					1273:1280	reduced total solids recovery	1252:1280	reduced total solids recovery in the curd and lower cheese yields	1252:1316	Results indicated that high levels of milk lactose were associated with reduced total solids recovery in the curd and lower cheese yields, because of the lower milk fat and protein contents in samples rich in lactose.
31351732	5	101	theme	protein	1353:1359	arg1	contents					1361:1368	protein contents	1353:1368	protein contents	1353:1368	Results indicated that high levels of milk lactose were associated with reduced total solids recovery in the curd and lower cheese yields, because of the lower milk fat and protein contents in samples rich in lactose.
31351732	2	102	from	species	586:592	arg1	conclusions					552:562	conclusions	552:562	conclusions from the most studied species (i.e., bovine)	552:607	Because large differences exist among dairy species, conclusions from the most studied species (i.e., bovine) cannot be drawn for all types of dairy-producing animals.
31351732	1	103	theme	cheese	303:308	arg1	yield					310:314	cheese yield	303:314	cheese yield	303:314	In dairy goats, very little is known about the effect of the 2 most important indirect indicators of udder health [somatic cell count (SCC) and total bacterial count (TBC)] on milk composition and cheese yield, and no information is available regarding the effects of lactose levels, pH, and NaCl content on the recovery of nutrients in the curd, cheese yield traits, and daily cheese yields.
31351732	10	104	dep	even	2004:2007	arg1	limits					2028:2033	the legal limits	2018:2033	the legal limits	2018:2033	Bacterial count proved to be the least important factor affecting cheese-making traits, but it decreased daily cheese yields, suggesting that, even if below the legal limits, TBC should be considered in order to monitor flock management and avoid economic losses.
31351732	3	105	dep	indicators	809:818	arg1	level					829:833	lactose level	821:833	lactose level	821:833	The aims of this study were to quantify, using milk samples from 560 dairy goats, the contemporary effects of a pool of udder health indirect indicators (lactose level, pH, SCC, TBC, and NaCl content) on the recovery of nutrients in the curd (%REC), cheese yield (%CY), and daily cheese yields (dCY).
31351732	2	106	theme	studied	578:584	arg1	species					586:592	the most studied species	569:592	the most studied species (i.e., bovine)	569:607	Because large differences exist among dairy species, conclusions from the most studied species (i.e., bovine) cannot be drawn for all types of dairy-producing animals.
31351732	6	107	theme	nutrients	1443:1451	arg1	recovery					1431:1438	higher recovery	1424:1438	higher recovery of nutrients in the curd and higher cheese yield traits	1424:1494	Higher pH correlated with higher recovery of nutrients in the curd and higher cheese yield traits.
31351732	5	108	theme	lactose	1223:1229	arg1	levels					1208:1213	high levels	1203:1213	high levels of milk lactose	1203:1229	Results indicated that high levels of milk lactose were associated with reduced total solids recovery in the curd and lower cheese yields, because of the lower milk fat and protein contents in samples rich in lactose.
31351732	7	109	theme	positive	1535:1542	arg1	correlation					1544:1554	the positive correlation	1531:1554	the positive correlation between pH and milk fat, protein, and casein in goat milk	1531:1612	These results may be explained by the positive correlation between pH and milk fat, protein, and casein in goat milk.
31351732	10	110	theme	Bacterial	1861:1869	arg1	factor					1910:1915	the least important factor	1890:1915	the least important factor affecting cheese-making traits	1890:1946	Bacterial count proved to be the least important factor affecting cheese-making traits, but it decreased daily cheese yields, suggesting that, even if below the legal limits, TBC should be considered in order to monitor flock management and avoid economic losses.
31351732	10	110	theme	Bacterial	1861:1869	arg1	count					1871:1875	Bacterial count	1861:1875	Bacterial count	1861:1875	Bacterial count proved to be the least important factor affecting cheese-making traits, but it decreased daily cheese yields, suggesting that, even if below the legal limits, TBC should be considered in order to monitor flock management and avoid economic losses.
31351732	3	111	from	effects	766:772	arg1	recovery					875:882	the recovery	871:882	the recovery of nutrients in the curd (%REC)	871:914	The aims of this study were to quantify, using milk samples from 560 dairy goats, the contemporary effects of a pool of udder health indirect indicators (lactose level, pH, SCC, TBC, and NaCl content) on the recovery of nutrients in the curd (%REC), cheese yield (%CY), and daily cheese yields (dCY).
31351732	9	112	theme	cheese	1736:1741	arg1	yield					1743:1747	The higher cheese yield	1725:1747	The higher cheese yield obtained from milk with high SCC	1725:1780	The higher cheese yield obtained from milk with high SCC was due to both increased recovery of lactose in the curd and water retention.
31351732	9	112	theme	cheese	1736:1741	arg1	due					1786:1788	due	1786:1788	due	1786:1788	The higher cheese yield obtained from milk with high SCC was due to both increased recovery of lactose in the curd and water retention.
31351732	0	113	from	traits	85:90	arg1	milk					100:103	goat milk	95:103	goat milk	95:103	Effects of indirect indicators of udder health on nutrient recovery and cheese yield traits in goat milk.
31351732	11	114	theme	milk	2155:2158	arg1	composition					2160:2170	milk composition	2155:2170	milk composition	2155:2170	The effect of NaCl content on milk composition was linked with lower recovery of all nutrients in the curd during cheese-making.
31351732	14	115	theme	payment	2540:2546	arg1	system					2548:2553	the milk payment system	2531:2553	the milk payment system	2531:2553	Such information should be considered, to adjust the milk-to-cheese economic value and the milk payment system.
31351732	11	116	from	recovery	2194:2201	arg1	curd					2227:2230	the curd	2223:2230	the curd during cheese-making	2223:2251	The effect of NaCl content on milk composition was linked with lower recovery of all nutrients in the curd during cheese-making.
31351732	12	117	theme	fresh	2311:2315	arg1	yield					2324:2328	fresh cheese yield	2311:2328	fresh cheese yield	2311:2328	In addition, high milk NaCl content led to reductions in fresh cheese yield and cheese solids.
31351732	3	118	from	goats	742:746	arg1	yield					924:928	cheese yield	917:928	cheese yield (%CY)	917:934	The aims of this study were to quantify, using milk samples from 560 dairy goats, the contemporary effects of a pool of udder health indirect indicators (lactose level, pH, SCC, TBC, and NaCl content) on the recovery of nutrients in the curd (%REC), cheese yield (%CY), and daily cheese yields (dCY).
31351732	3	118	from	goats	742:746	arg1	effects					766:772	the contemporary effects	749:772	the contemporary effects of a pool of udder health indirect indicators (lactose level, pH, SCC, TBC, and NaCl content) on the recovery of nutrients in the curd (%REC)	749:914	The aims of this study were to quantify, using milk samples from 560 dairy goats, the contemporary effects of a pool of udder health indirect indicators (lactose level, pH, SCC, TBC, and NaCl content) on the recovery of nutrients in the curd (%REC), cheese yield (%CY), and daily cheese yields (dCY).
31351732	3	118	from	goats	742:746	arg1	CY					932:933	%CY	931:933	%CY	931:933	The aims of this study were to quantify, using milk samples from 560 dairy goats, the contemporary effects of a pool of udder health indirect indicators (lactose level, pH, SCC, TBC, and NaCl content) on the recovery of nutrients in the curd (%REC), cheese yield (%CY), and daily cheese yields (dCY).
31351732	3	118	from	goats	742:746	arg1	yields					954:959	daily cheese yields	941:959	daily cheese yields (dCY)	941:965	The aims of this study were to quantify, using milk samples from 560 dairy goats, the contemporary effects of a pool of udder health indirect indicators (lactose level, pH, SCC, TBC, and NaCl content) on the recovery of nutrients in the curd (%REC), cheese yield (%CY), and daily cheese yields (dCY).
31351732	3	118	from	goats	742:746	arg1	dCY					962:964	dCY	962:964	dCY	962:964	The aims of this study were to quantify, using milk samples from 560 dairy goats, the contemporary effects of a pool of udder health indirect indicators (lactose level, pH, SCC, TBC, and NaCl content) on the recovery of nutrients in the curd (%REC), cheese yield (%CY), and daily cheese yields (dCY).
31351732	3	118	from	goats	742:746	arg1	samples					719:725	milk samples	714:725	milk samples from 560 dairy goats	714:746	The aims of this study were to quantify, using milk samples from 560 dairy goats, the contemporary effects of a pool of udder health indirect indicators (lactose level, pH, SCC, TBC, and NaCl content) on the recovery of nutrients in the curd (%REC), cheese yield (%CY), and daily cheese yields (dCY).
31351732	4	119	from	tube	1144:1147	arg1	DIM					1051:1053	DIM	1051:1053	DIM	1051:1053	Cheese-making traits were analyzed using a mixed model, with parity, days in milk (DIM), lactose level, pH, SCC, TBC, and NaCl content as fixed effects, and farm, breed, glass tube, and animal as random effects.
31351732	4	119	from	tube	1144:1147	arg1	farm					1125:1128	farm	1125:1128	farm	1125:1128	Cheese-making traits were analyzed using a mixed model, with parity, days in milk (DIM), lactose level, pH, SCC, TBC, and NaCl content as fixed effects, and farm, breed, glass tube, and animal as random effects.
31351732	4	119	from	tube	1144:1147	arg1	milk					1045:1048	milk	1045:1048	milk (DIM)	1045:1054	Cheese-making traits were analyzed using a mixed model, with parity, days in milk (DIM), lactose level, pH, SCC, TBC, and NaCl content as fixed effects, and farm, breed, glass tube, and animal as random effects.
31351732	4	119	from	tube	1144:1147	arg1	level					1065:1069	lactose level	1057:1069	lactose level	1057:1069	Cheese-making traits were analyzed using a mixed model, with parity, days in milk (DIM), lactose level, pH, SCC, TBC, and NaCl content as fixed effects, and farm, breed, glass tube, and animal as random effects.
31351732	11	120	from	effect	2129:2134	arg1	composition					2160:2170	milk composition	2155:2170	milk composition	2155:2170	The effect of NaCl content on milk composition was linked with lower recovery of all nutrients in the curd during cheese-making.
31351732	5	121	from	lactose	1389:1395	arg1	rich					1381:1384	rich	1381:1384	rich	1381:1384	Results indicated that high levels of milk lactose were associated with reduced total solids recovery in the curd and lower cheese yields, because of the lower milk fat and protein contents in samples rich in lactose.
31351732	3	122	from	recovery	875:882	arg1	curd					904:907	the curd	900:907	the curd (%REC)	900:914	The aims of this study were to quantify, using milk samples from 560 dairy goats, the contemporary effects of a pool of udder health indirect indicators (lactose level, pH, SCC, TBC, and NaCl content) on the recovery of nutrients in the curd (%REC), cheese yield (%CY), and daily cheese yields (dCY).
31351732	3	122	from	recovery	875:882	arg1	REC					911:913	%REC	910:913	%REC	910:913	The aims of this study were to quantify, using milk samples from 560 dairy goats, the contemporary effects of a pool of udder health indirect indicators (lactose level, pH, SCC, TBC, and NaCl content) on the recovery of nutrients in the curd (%REC), cheese yield (%CY), and daily cheese yields (dCY).
31351732	4	123	from	days	1037:1040	arg1	DIM					1051:1053	DIM	1051:1053	DIM	1051:1053	Cheese-making traits were analyzed using a mixed model, with parity, days in milk (DIM), lactose level, pH, SCC, TBC, and NaCl content as fixed effects, and farm, breed, glass tube, and animal as random effects.
31351732	4	123	from	days	1037:1040	arg1	farm					1125:1128	farm	1125:1128	farm	1125:1128	Cheese-making traits were analyzed using a mixed model, with parity, days in milk (DIM), lactose level, pH, SCC, TBC, and NaCl content as fixed effects, and farm, breed, glass tube, and animal as random effects.
31351732	4	123	from	days	1037:1040	arg1	milk					1045:1048	milk	1045:1048	milk (DIM)	1045:1054	Cheese-making traits were analyzed using a mixed model, with parity, days in milk (DIM), lactose level, pH, SCC, TBC, and NaCl content as fixed effects, and farm, breed, glass tube, and animal as random effects.
31351732	4	123	from	days	1037:1040	arg1	level					1065:1069	lactose level	1057:1069	lactose level	1057:1069	Cheese-making traits were analyzed using a mixed model, with parity, days in milk (DIM), lactose level, pH, SCC, TBC, and NaCl content as fixed effects, and farm, breed, glass tube, and animal as random effects.
31351732	9	124	theme	high	1773:1776	arg1	SCC					1778:1780	high SCC	1773:1780	high SCC	1773:1780	The higher cheese yield obtained from milk with high SCC was due to both increased recovery of lactose in the curd and water retention.
31351732	5	125	from	rich	1381:1384	arg1	lactose					1389:1395	lactose	1389:1395	lactose	1389:1395	Results indicated that high levels of milk lactose were associated with reduced total solids recovery in the curd and lower cheese yields, because of the lower milk fat and protein contents in samples rich in lactose.
31351732	8	126	from	recovery	1652:1659	arg1	recovery					1704:1711	the curd but lower recovery	1685:1711	the curd but lower recovery of protein	1685:1722	High SCC were associated with higher recovery of solids and energy in the curd but lower recovery of protein.
31351732	4	127	from	parity	1029:1034	arg1	DIM					1051:1053	DIM	1051:1053	DIM	1051:1053	Cheese-making traits were analyzed using a mixed model, with parity, days in milk (DIM), lactose level, pH, SCC, TBC, and NaCl content as fixed effects, and farm, breed, glass tube, and animal as random effects.
31351732	4	127	from	parity	1029:1034	arg1	farm					1125:1128	farm	1125:1128	farm	1125:1128	Cheese-making traits were analyzed using a mixed model, with parity, days in milk (DIM), lactose level, pH, SCC, TBC, and NaCl content as fixed effects, and farm, breed, glass tube, and animal as random effects.
31351732	4	127	from	parity	1029:1034	arg1	milk					1045:1048	milk	1045:1048	milk (DIM)	1045:1054	Cheese-making traits were analyzed using a mixed model, with parity, days in milk (DIM), lactose level, pH, SCC, TBC, and NaCl content as fixed effects, and farm, breed, glass tube, and animal as random effects.
31351732	4	127	from	parity	1029:1034	arg1	level					1065:1069	lactose level	1057:1069	lactose level	1057:1069	Cheese-making traits were analyzed using a mixed model, with parity, days in milk (DIM), lactose level, pH, SCC, TBC, and NaCl content as fixed effects, and farm, breed, glass tube, and animal as random effects.
31351732	0	128	theme	goat	95:98	arg1	milk					100:103	goat milk	95:103	goat milk	95:103	Effects of indirect indicators of udder health on nutrient recovery and cheese yield traits in goat milk.
31351732	12	129	from	reductions	2297:2306	arg1	yield					2324:2328	fresh cheese yield	2311:2328	fresh cheese yield	2311:2328	In addition, high milk NaCl content led to reductions in fresh cheese yield and cheese solids.
31351732	12	129	from	reductions	2297:2306	arg1	solids					2341:2346	cheese solids	2334:2346	cheese solids	2334:2346	In addition, high milk NaCl content led to reductions in fresh cheese yield and cheese solids.
31351732	8	130	theme	High	1615:1618	arg1	SCC					1620:1622	High SCC	1615:1622	High SCC	1615:1622	High SCC were associated with higher recovery of solids and energy in the curd but lower recovery of protein.
31351732	14	131	theme	milk-to-cheese	2497:2510	arg1	value					2521:2525	the milk-to-cheese economic value	2493:2525	the milk-to-cheese economic value	2493:2525	Such information should be considered, to adjust the milk-to-cheese economic value and the milk payment system.
31351732	12	132	theme	high	2267:2270	arg1	content					2282:2288	high milk NaCl content	2267:2288	high milk NaCl content	2267:2288	In addition, high milk NaCl content led to reductions in fresh cheese yield and cheese solids.
31351732	5	133	theme	cheese	1304:1309	arg1	yields					1311:1316	lower cheese yields	1298:1316	lower cheese yields	1298:1316	Results indicated that high levels of milk lactose were associated with reduced total solids recovery in the curd and lower cheese yields, because of the lower milk fat and protein contents in samples rich in lactose.
31351732	0	134	theme	indicators	20:29	arg1	Effects					0:6	Effects	0:6	Effects of indirect indicators of udder health on nutrient recovery and cheese	0:77	Effects of indirect indicators of udder health on nutrient recovery and cheese yield traits in goat milk.
31351732	1	135	theme	count	234:238	arg1	indicators					193:202	the 2 most important indirect indicators	163:202	the 2 most important indirect indicators of udder health [somatic cell count (SCC) and total bacterial count (TBC)]	163:277	In dairy goats, very little is known about the effect of the 2 most important indirect indicators of udder health [somatic cell count (SCC) and total bacterial count (TBC)] on milk composition and cheese yield, and no information is available regarding the effects of lactose levels, pH, and NaCl content on the recovery of nutrients in the curd, cheese yield traits, and daily cheese yields.
31351732	3	136	theme	nutrients	887:895	arg1	recovery					875:882	the recovery	871:882	the recovery of nutrients in the curd (%REC)	871:914	The aims of this study were to quantify, using milk samples from 560 dairy goats, the contemporary effects of a pool of udder health indirect indicators (lactose level, pH, SCC, TBC, and NaCl content) on the recovery of nutrients in the curd (%REC), cheese yield (%CY), and daily cheese yields (dCY).
31351732	2	137	dep	bovine	601:606	arg1	i.e.					595:598	i.e.	595:598	i.e.	595:598	Because large differences exist among dairy species, conclusions from the most studied species (i.e., bovine) cannot be drawn for all types of dairy-producing animals.
31351732	10	138	theme	flock	2081:2085	arg1	management					2087:2096	flock management	2081:2096	flock management	2081:2096	Bacterial count proved to be the least important factor affecting cheese-making traits, but it decreased daily cheese yields, suggesting that, even if below the legal limits, TBC should be considered in order to monitor flock management and avoid economic losses.
31351732	1	139	theme	cheese	453:458	arg1	traits					466:471	cheese yield traits	453:471	cheese yield traits	453:471	In dairy goats, very little is known about the effect of the 2 most important indirect indicators of udder health [somatic cell count (SCC) and total bacterial count (TBC)] on milk composition and cheese yield, and no information is available regarding the effects of lactose levels, pH, and NaCl content on the recovery of nutrients in the curd, cheese yield traits, and daily cheese yields.
31351732	0	140	theme	udder	34:38	arg1	health					40:45	udder health	34:45	udder health	34:45	Effects of indirect indicators of udder health on nutrient recovery and cheese yield traits in goat milk.
31351732	7	141	from	protein	1581:1587	arg1	milk					1609:1612	goat milk	1604:1612	goat milk	1604:1612	These results may be explained by the positive correlation between pH and milk fat, protein, and casein in goat milk.
31351732	11	142	theme	NaCl	2139:2142	arg1	content					2144:2150	NaCl content	2139:2150	NaCl content	2139:2150	The effect of NaCl content on milk composition was linked with lower recovery of all nutrients in the curd during cheese-making.
31351732	1	143	theme	pH	390:391	arg1	yields					491:496	daily cheese yields	478:496	daily cheese yields	478:496	In dairy goats, very little is known about the effect of the 2 most important indirect indicators of udder health [somatic cell count (SCC) and total bacterial count (TBC)] on milk composition and cheese yield, and no information is available regarding the effects of lactose levels, pH, and NaCl content on the recovery of nutrients in the curd, cheese yield traits, and daily cheese yields.
31351732	1	143	theme	pH	390:391	arg1	traits					466:471	cheese yield traits	453:471	cheese yield traits	453:471	In dairy goats, very little is known about the effect of the 2 most important indirect indicators of udder health [somatic cell count (SCC) and total bacterial count (TBC)] on milk composition and cheese yield, and no information is available regarding the effects of lactose levels, pH, and NaCl content on the recovery of nutrients in the curd, cheese yield traits, and daily cheese yields.
31351732	1	143	theme	pH	390:391	arg1	effects					363:369	the effects	359:369	the effects of lactose levels, pH, and NaCl content on the recovery of nutrients in the curd	359:450	In dairy goats, very little is known about the effect of the 2 most important indirect indicators of udder health [somatic cell count (SCC) and total bacterial count (TBC)] on milk composition and cheese yield, and no information is available regarding the effects of lactose levels, pH, and NaCl content on the recovery of nutrients in the curd, cheese yield traits, and daily cheese yields.
31351732	4	144	theme	fixed	1106:1110	arg1	effects					1112:1118	fixed effects	1106:1118	fixed effects	1106:1118	Cheese-making traits were analyzed using a mixed model, with parity, days in milk (DIM), lactose level, pH, SCC, TBC, and NaCl content as fixed effects, and farm, breed, glass tube, and animal as random effects.
31351732	8	145	theme	solids	1664:1669	arg1	recovery					1652:1659	higher recovery	1645:1659	higher recovery of solids and energy in the curd but lower recovery of protein	1645:1722	High SCC were associated with higher recovery of solids and energy in the curd but lower recovery of protein.
31351732	10	146	theme	economic	2108:2115	arg1	losses					2117:2122	economic losses	2108:2122	economic losses	2108:2122	Bacterial count proved to be the least important factor affecting cheese-making traits, but it decreased daily cheese yields, suggesting that, even if below the legal limits, TBC should be considered in order to monitor flock management and avoid economic losses.
31351732	5	147	theme	lower	1334:1338	arg1	fat					1345:1347	the lower milk fat	1330:1347	the lower milk fat	1330:1347	Results indicated that high levels of milk lactose were associated with reduced total solids recovery in the curd and lower cheese yields, because of the lower milk fat and protein contents in samples rich in lactose.
31351732	1	148	theme	bacterial	256:264	arg1	TBC					273:275	TBC	273:275	TBC	273:275	In dairy goats, very little is known about the effect of the 2 most important indirect indicators of udder health [somatic cell count (SCC) and total bacterial count (TBC)] on milk composition and cheese yield, and no information is available regarding the effects of lactose levels, pH, and NaCl content on the recovery of nutrients in the curd, cheese yield traits, and daily cheese yields.
31351732	1	148	theme	bacterial	256:264	arg1	count					266:270	total bacterial count	250:270	total bacterial count (TBC)]	250:277	In dairy goats, very little is known about the effect of the 2 most important indirect indicators of udder health [somatic cell count (SCC) and total bacterial count (TBC)] on milk composition and cheese yield, and no information is available regarding the effects of lactose levels, pH, and NaCl content on the recovery of nutrients in the curd, cheese yield traits, and daily cheese yields.
31351732	13	149	theme	study	2388:2392	arg1	indicators					2362:2371	The indirect indicators	2349:2371	The indirect indicators of the present study	2349:2392	The indirect indicators of the present study significantly affected the cheese-making process.
31351732	5	150	from	recovery	1273:1280	arg1	yields					1311:1316	lower cheese yields	1298:1316	lower cheese yields	1298:1316	Results indicated that high levels of milk lactose were associated with reduced total solids recovery in the curd and lower cheese yields, because of the lower milk fat and protein contents in samples rich in lactose.
31351732	5	150	from	recovery	1273:1280	arg1	curd					1289:1292	curd	1289:1292	curd	1289:1292	Results indicated that high levels of milk lactose were associated with reduced total solids recovery in the curd and lower cheese yields, because of the lower milk fat and protein contents in samples rich in lactose.
31351732	8	151	theme	energy	1675:1680	arg1	recovery					1652:1659	higher recovery	1645:1659	higher recovery of solids and energy in the curd but lower recovery of protein	1645:1722	High SCC were associated with higher recovery of solids and energy in the curd but lower recovery of protein.
31351732	2	152	theme	animals	658:664	arg1	types					633:637	all types	629:637	all types of dairy-producing animals	629:664	Because large differences exist among dairy species, conclusions from the most studied species (i.e., bovine) cannot be drawn for all types of dairy-producing animals.
31351732	1	153	theme	content	403:409	arg1	yields					491:496	daily cheese yields	478:496	daily cheese yields	478:496	In dairy goats, very little is known about the effect of the 2 most important indirect indicators of udder health [somatic cell count (SCC) and total bacterial count (TBC)] on milk composition and cheese yield, and no information is available regarding the effects of lactose levels, pH, and NaCl content on the recovery of nutrients in the curd, cheese yield traits, and daily cheese yields.
31351732	1	153	theme	content	403:409	arg1	traits					466:471	cheese yield traits	453:471	cheese yield traits	453:471	In dairy goats, very little is known about the effect of the 2 most important indirect indicators of udder health [somatic cell count (SCC) and total bacterial count (TBC)] on milk composition and cheese yield, and no information is available regarding the effects of lactose levels, pH, and NaCl content on the recovery of nutrients in the curd, cheese yield traits, and daily cheese yields.
31351732	1	153	theme	content	403:409	arg1	effects					363:369	the effects	359:369	the effects of lactose levels, pH, and NaCl content on the recovery of nutrients in the curd	359:450	In dairy goats, very little is known about the effect of the 2 most important indirect indicators of udder health [somatic cell count (SCC) and total bacterial count (TBC)] on milk composition and cheese yield, and no information is available regarding the effects of lactose levels, pH, and NaCl content on the recovery of nutrients in the curd, cheese yield traits, and daily cheese yields.
31351732	9	154	theme	water	1844:1848	arg1	retention					1850:1858	water retention	1844:1858	water retention	1844:1858	The higher cheese yield obtained from milk with high SCC was due to both increased recovery of lactose in the curd and water retention.
31351732	3	155	dep	level	829:833	arg1	TBC					845:847	TBC	845:847	TBC	845:847	The aims of this study were to quantify, using milk samples from 560 dairy goats, the contemporary effects of a pool of udder health indirect indicators (lactose level, pH, SCC, TBC, and NaCl content) on the recovery of nutrients in the curd (%REC), cheese yield (%CY), and daily cheese yields (dCY).
31351732	3	155	dep	level	829:833	arg1	SCC					840:842	SCC	840:842	SCC	840:842	The aims of this study were to quantify, using milk samples from 560 dairy goats, the contemporary effects of a pool of udder health indirect indicators (lactose level, pH, SCC, TBC, and NaCl content) on the recovery of nutrients in the curd (%REC), cheese yield (%CY), and daily cheese yields (dCY).
31351732	3	155	dep	level	829:833	arg1	content					859:865	NaCl content	854:865	NaCl content	854:865	The aims of this study were to quantify, using milk samples from 560 dairy goats, the contemporary effects of a pool of udder health indirect indicators (lactose level, pH, SCC, TBC, and NaCl content) on the recovery of nutrients in the curd (%REC), cheese yield (%CY), and daily cheese yields (dCY).
31351732	3	155	dep	level	829:833	arg1	pH					836:837	pH	836:837	pH	836:837	The aims of this study were to quantify, using milk samples from 560 dairy goats, the contemporary effects of a pool of udder health indirect indicators (lactose level, pH, SCC, TBC, and NaCl content) on the recovery of nutrients in the curd (%REC), cheese yield (%CY), and daily cheese yields (dCY).
31351732	1	156	theme	important	174:182	arg1	indicators					193:202	the 2 most important indirect indicators	163:202	the 2 most important indirect indicators of udder health [somatic cell count (SCC) and total bacterial count (TBC)]	163:277	In dairy goats, very little is known about the effect of the 2 most important indirect indicators of udder health [somatic cell count (SCC) and total bacterial count (TBC)] on milk composition and cheese yield, and no information is available regarding the effects of lactose levels, pH, and NaCl content on the recovery of nutrients in the curd, cheese yield traits, and daily cheese yields.
31351732	7	157	theme	goat	1604:1607	arg1	milk					1609:1612	goat milk	1604:1612	goat milk	1604:1612	These results may be explained by the positive correlation between pH and milk fat, protein, and casein in goat milk.
31351732	3	158	theme	indicators	809:818	arg1	pool					779:782	a pool	777:782	a pool of udder health indirect indicators (lactose level, pH, SCC, TBC, and NaCl content)	777:866	The aims of this study were to quantify, using milk samples from 560 dairy goats, the contemporary effects of a pool of udder health indirect indicators (lactose level, pH, SCC, TBC, and NaCl content) on the recovery of nutrients in the curd (%REC), cheese yield (%CY), and daily cheese yields (dCY).
31351732	6	159	theme	higher	1424:1429	arg1	recovery					1431:1438	higher recovery	1424:1438	higher recovery of nutrients in the curd and higher cheese yield traits	1424:1494	Higher pH correlated with higher recovery of nutrients in the curd and higher cheese yield traits.
31351732	12	160	theme	cheese	2334:2339	arg1	solids					2341:2346	cheese solids	2334:2346	cheese solids	2334:2346	In addition, high milk NaCl content led to reductions in fresh cheese yield and cheese solids.
31351732	1	161	theme	indicators	193:202	arg1	effect					153:158	the effect	149:158	the effect of the 2 most important indirect indicators of udder health [somatic cell count (SCC) and total bacterial count (TBC)] on milk composition and cheese yield	149:314	In dairy goats, very little is known about the effect of the 2 most important indirect indicators of udder health [somatic cell count (SCC) and total bacterial count (TBC)] on milk composition and cheese yield, and no information is available regarding the effects of lactose levels, pH, and NaCl content on the recovery of nutrients in the curd, cheese yield traits, and daily cheese yields.
30451726	11	0	theme	experiment	1871:1880	arg1	end					1860:1862	the end	1856:1862	the end of the experiment	1856:1880	Syndecan-1 significantly increased in NTHS (mean difference, -1668; 95% confidence interval [CI], -2336 to -1001; P < .0001), balanced crystalloid (mean difference, -964.2; 95% CI, -1492 to -436.4; P = .0001), and HES (mean difference, -1030; 95% CI, -1594 to -465.8; P = .0001) groups at the end of the experiment compared to baseline.
30451726	11	1	dep	difference	1720:1729	arg1	CI					1744:1745	95% CI	1740:1745	95% CI	1740:1745	Syndecan-1 significantly increased in NTHS (mean difference, -1668; 95% confidence interval [CI], -2336 to -1001; P < .0001), balanced crystalloid (mean difference, -964.2; 95% CI, -1492 to -436.4; P = .0001), and HES (mean difference, -1030; 95% CI, -1594 to -465.8; P = .0001) groups at the end of the experiment compared to baseline.
30451726	11	1	dep	difference	1720:1729	arg1	P					1765:1765	P = .0001	1765:1773	P = .0001	1765:1773	Syndecan-1 significantly increased in NTHS (mean difference, -1668; 95% confidence interval [CI], -2336 to -1001; P < .0001), balanced crystalloid (mean difference, -964.2; 95% CI, -1492 to -436.4; P = .0001), and HES (mean difference, -1030; 95% CI, -1594 to -465.8; P = .0001) groups at the end of the experiment compared to baseline.
30451726	11	1	dep	difference	1720:1729	arg1	-964.2					1732:1737	-964.2	1732:1737	-964.2	1732:1737	Syndecan-1 significantly increased in NTHS (mean difference, -1668; 95% confidence interval [CI], -2336 to -1001; P < .0001), balanced crystalloid (mean difference, -964.2; 95% CI, -1492 to -436.4; P = .0001), and HES (mean difference, -1030; 95% CI, -1594 to -465.8; P = .0001) groups at the end of the experiment compared to baseline.
30451726	11	1	dep	difference	1720:1729	arg1	-436.4					1757:1762	-436.4	1757:1762	-436.4	1757:1762	Syndecan-1 significantly increased in NTHS (mean difference, -1668; 95% confidence interval [CI], -2336 to -1001; P < .0001), balanced crystalloid (mean difference, -964.2; 95% CI, -1492 to -436.4; P = .0001), and HES (mean difference, -1030; 95% CI, -1594 to -465.8; P = .0001) groups at the end of the experiment compared to baseline.
30451726	6	2	theme	Hg	1030:1031	arg1	pressure					1012:1019	a mean arterial pressure	996:1019	a mean arterial pressure of 80 mm Hg	996:1031	Animals were fluid resuscitated with Ringer's acetate, balanced hydroxyethyl starch (HES) solution, or 0.9% normal saline to a mean arterial pressure of 80 mm Hg and compared with shams or nonresuscitated NTHS.
30451726	2	3	theme	hemorrhagic	359:369	arg1	shock					371:375	nontraumatic hemorrhagic shock	346:375	nontraumatic hemorrhagic shock (NTHS)	346:382	In nontraumatic hemorrhagic shock (NTHS), it remains questionable whether glycocalyx degradation in itself results in an alteration of VBP.
30451726	2	3	theme	hemorrhagic	359:369	arg1	NTHS					378:381	NTHS	378:381	NTHS	378:381	In nontraumatic hemorrhagic shock (NTHS), it remains questionable whether glycocalyx degradation in itself results in an alteration of VBP.
30451726	14	4	theme	vessel	2290:2295	arg1	density					2297:2303	Total vessel density	2284:2303	Total vessel density	2284:2303	Total vessel density was altered in the normal saline (mean difference, 4.092; 95% CI, 0.6195-7.564; P = .016) and hemorrhagic shock (mean difference, 5.022; 95% CI, 1.55-8.495; P = .0024) groups compared to the control group, as well as the perfused vessel density and mean flow index.
30451726	10	5	theme	shedding	1514:1521	arg1	result					1551:1556	a result	1549:1556	a result of NTHS	1549:1564	RESULTS All glycocalyx shedding products were upgraded as a result of NTHS.
30451726	10	5	theme	shedding	1514:1521	arg1	products					1523:1530	All glycocalyx shedding products	1499:1530	All glycocalyx shedding products	1499:1530	RESULTS All glycocalyx shedding products were upgraded as a result of NTHS.
30451726	11	6	theme	mean	1611:1614	arg1	NTHS					1605:1608	NTHS	1605:1608	NTHS (mean difference, -1668; 95% confidence interval [CI], -2336 to -1001; P < .0001)	1605:1690	Syndecan-1 significantly increased in NTHS (mean difference, -1668; 95% confidence interval [CI], -2336 to -1001; P < .0001), balanced crystalloid (mean difference, -964.2; 95% CI, -1492 to -436.4; P = .0001), and HES (mean difference, -1030; 95% CI, -1594 to -465.8; P = .0001) groups at the end of the experiment compared to baseline.
30451726	11	6	theme	mean	1611:1614	arg1	difference					1616:1625	mean difference	1611:1625	mean difference	1611:1625	Syndecan-1 significantly increased in NTHS (mean difference, -1668; 95% confidence interval [CI], -2336 to -1001; P < .0001), balanced crystalloid (mean difference, -964.2; 95% CI, -1492 to -436.4; P = .0001), and HES (mean difference, -1030; 95% CI, -1594 to -465.8; P = .0001) groups at the end of the experiment compared to baseline.
30451726	11	7	theme	%	1742:1742	arg1	CI					1744:1745	95% CI	1740:1745	95% CI	1740:1745	Syndecan-1 significantly increased in NTHS (mean difference, -1668; 95% confidence interval [CI], -2336 to -1001; P < .0001), balanced crystalloid (mean difference, -964.2; 95% CI, -1492 to -436.4; P = .0001), and HES (mean difference, -1030; 95% CI, -1594 to -465.8; P = .0001) groups at the end of the experiment compared to baseline.
30451726	1	8	theme	vascular	280:287	arg1	VBP					311:313	VBP	311:313	VBP	311:313	BACKGROUND Glycocalyx shedding after traumatic hemorrhagic or septic shock, as well as different resuscitation fluids, has been causally linked to increased vascular barrier permeability (VBP) resulting in tissue edema.
30451726	1	8	theme	vascular	280:287	arg1	permeability					297:308	vascular barrier permeability	280:308	increased vascular barrier permeability (VBP) resulting in tissue edema	270:340	BACKGROUND Glycocalyx shedding after traumatic hemorrhagic or septic shock, as well as different resuscitation fluids, has been causally linked to increased vascular barrier permeability (VBP) resulting in tissue edema.
30451726	12	9	dep	higher	1927:1932	arg1	-923.1					1988:1993	-923.1	1988:1993	-923.1	1988:1993	Hyaluronan levels were higher at the end of the experiment in nonresuscitated NTHS (-923.1; 95% CI, -1216 to -630; P = .0001) and balanced crystalloid (-1039; 95% CI, -1332 to -745.5; P = .0001) or HES (-394.2; 95% CI, -670.1 to -118.3; P = .0027) groups compared to controls.
30451726	14	10	theme	mean	2418:2421	arg1	difference					2423:2432	mean difference	2418:2432	mean difference	2418:2432	Total vessel density was altered in the normal saline (mean difference, 4.092; 95% CI, 0.6195-7.564; P = .016) and hemorrhagic shock (mean difference, 5.022; 95% CI, 1.55-8.495; P = .0024) groups compared to the control group, as well as the perfused vessel density and mean flow index.
30451726	8	11	theme	muscle	1186:1191	arg1	microcirculation					1193:1208	Skeletal muscle microcirculation	1177:1208	Skeletal muscle microcirculation	1177:1208	Skeletal muscle microcirculation was visualized using handheld vital microscopy.
30451726	11	12	theme	NTHS	1605:1608	arg1	difference					1791:1800	mean difference	1786:1800	mean difference	1786:1800	Syndecan-1 significantly increased in NTHS (mean difference, -1668; 95% confidence interval [CI], -2336 to -1001; P < .0001), balanced crystalloid (mean difference, -964.2; 95% CI, -1492 to -436.4; P = .0001), and HES (mean difference, -1030; 95% CI, -1594 to -465.8; P = .0001) groups at the end of the experiment compared to baseline.
30451726	11	12	theme	NTHS	1605:1608	arg1	groups					1846:1851	NTHS (mean difference, -1668; 95% confidence interval [CI], -2336 to -1001; P < .0001), balanced crystalloid (mean difference, -964.2; 95% CI, -1492 to -436.4; P = .0001), and HES (mean difference, -1030; 95% CI, -1594 to -465.8; P = .0001) groups	1605:1851	NTHS (mean difference, -1668; 95% confidence interval [CI], -2336 to -1001; P < .0001), balanced crystalloid (mean difference, -964.2; 95% CI, -1492 to -436.4; P = .0001), and HES (mean difference, -1030; 95% CI, -1594 to -465.8; P = .0001) groups	1605:1851	Syndecan-1 significantly increased in NTHS (mean difference, -1668; 95% confidence interval [CI], -2336 to -1001; P < .0001), balanced crystalloid (mean difference, -964.2; 95% CI, -1492 to -436.4; P = .0001), and HES (mean difference, -1030; 95% CI, -1594 to -465.8; P = .0001) groups at the end of the experiment compared to baseline.
30451726	14	13	theme	control	2496:2502	arg1	group					2504:2508	the control group	2492:2508	the control group	2492:2508	Total vessel density was altered in the normal saline (mean difference, 4.092; 95% CI, 0.6195-7.564; P = .016) and hemorrhagic shock (mean difference, 5.022; 95% CI, 1.55-8.495; P = .0024) groups compared to the control group, as well as the perfused vessel density and mean flow index.
30451726	3	14	theme	modulatory	525:534	arg1	effect					536:541	a modulatory effect	523:541	a modulatory effect	523:541	The composition of fluids can also have a modulatory effect on glycocalyx shedding and VBP.
30451726	9	15	theme	dye	1382:1384	arg1	exclusion					1386:1394	Evans blue dye exclusion	1371:1394	Evans blue dye exclusion	1371:1394	VBP changes were assessed using plasma decay of 3 fluorescent dyes (40- and 500-kDa dextran and 70-kDa albumin), Evans blue dye exclusion, intravital fluorescence microscopy, and determination of tissue edema (wet/dry weight ratio).
30451726	14	16	theme	shock	2411:2415	arg1	groups					2473:2478	the normal saline (mean difference, 4.092; 95% CI, 0.6195-7.564; P = .016) and hemorrhagic shock (mean difference, 5.022; 95% CI, 1.55-8.495; P = .0024) groups	2320:2478	the normal saline (mean difference, 4.092; 95% CI, 0.6195-7.564; P = .016) and hemorrhagic shock (mean difference, 5.022; 95% CI, 1.55-8.495; P = .0024) groups	2320:2478	Total vessel density was altered in the normal saline (mean difference, 4.092; 95% CI, 0.6195-7.564; P = .016) and hemorrhagic shock (mean difference, 5.022; 95% CI, 1.55-8.495; P = .0024) groups compared to the control group, as well as the perfused vessel density and mean flow index.
30451726	12	17	theme	Hyaluronan	1904:1913	arg1	levels					1915:1920	Hyaluronan levels	1904:1920	Hyaluronan levels	1904:1920	Hyaluronan levels were higher at the end of the experiment in nonresuscitated NTHS (-923.1; 95% CI, -1216 to -630; P = .0001) and balanced crystalloid (-1039; 95% CI, -1332 to -745.5; P = .0001) or HES (-394.2; 95% CI, -670.1 to -118.3; P = .0027) groups compared to controls.
30451726	5	18	theme	Hg	851:852	arg1	pressure					833:840	mean arterial pressure	819:840	mean arterial pressure of 30 mm Hg	819:852	METHODS Fully instrumented Wistar-albino rats were subjected to a pressure-controlled NTHS (mean arterial pressure of 30 mm Hg) for 60 minutes.
30451726	6	19	theme	starch	948:953	arg1	solution					961:968	balanced hydroxyethyl starch (HES) solution	926:968	balanced hydroxyethyl starch (HES) solution	926:968	Animals were fluid resuscitated with Ringer's acetate, balanced hydroxyethyl starch (HES) solution, or 0.9% normal saline to a mean arterial pressure of 80 mm Hg and compared with shams or nonresuscitated NTHS.
30451726	14	20	theme	Total	2284:2288	arg1	density					2297:2303	Total vessel density	2284:2303	Total vessel density	2284:2303	Total vessel density was altered in the normal saline (mean difference, 4.092; 95% CI, 0.6195-7.564; P = .016) and hemorrhagic shock (mean difference, 5.022; 95% CI, 1.55-8.495; P = .0024) groups compared to the control group, as well as the perfused vessel density and mean flow index.
30451726	9	21	theme	fluorescence	1408:1419	arg1	microscopy					1421:1430	intravital fluorescence microscopy	1397:1430	intravital fluorescence microscopy	1397:1430	VBP changes were assessed using plasma decay of 3 fluorescent dyes (40- and 500-kDa dextran and 70-kDa albumin), Evans blue dye exclusion, intravital fluorescence microscopy, and determination of tissue edema (wet/dry weight ratio).
30451726	6	22	theme	balanced	926:933	arg1	solution					961:968	balanced hydroxyethyl starch (HES) solution	926:968	balanced hydroxyethyl starch (HES) solution	926:968	Animals were fluid resuscitated with Ringer's acetate, balanced hydroxyethyl starch (HES) solution, or 0.9% normal saline to a mean arterial pressure of 80 mm Hg and compared with shams or nonresuscitated NTHS.
30451726	8	23	theme	vital	1240:1244	arg1	microscopy					1246:1255	handheld vital microscopy	1231:1255	handheld vital microscopy	1231:1255	Skeletal muscle microcirculation was visualized using handheld vital microscopy.
30451726	10	24	theme	NTHS	1561:1564	arg1	result					1551:1556	a result	1549:1556	a result of NTHS	1549:1564	RESULTS All glycocalyx shedding products were upgraded as a result of NTHS.
30451726	10	24	theme	NTHS	1561:1564	arg1	products					1523:1530	All glycocalyx shedding products	1499:1530	All glycocalyx shedding products	1499:1530	RESULTS All glycocalyx shedding products were upgraded as a result of NTHS.
30451726	11	25	theme	mean	1715:1718	arg1	crystalloid					1702:1712	balanced crystalloid	1693:1712	balanced crystalloid (mean difference, -964.2; 95% CI, -1492 to -436.4; P = .0001)	1693:1774	Syndecan-1 significantly increased in NTHS (mean difference, -1668; 95% confidence interval [CI], -2336 to -1001; P < .0001), balanced crystalloid (mean difference, -964.2; 95% CI, -1492 to -436.4; P = .0001), and HES (mean difference, -1030; 95% CI, -1594 to -465.8; P = .0001) groups at the end of the experiment compared to baseline.
30451726	11	25	theme	mean	1715:1718	arg1	difference					1720:1729	mean difference	1715:1729	mean difference	1715:1729	Syndecan-1 significantly increased in NTHS (mean difference, -1668; 95% confidence interval [CI], -2336 to -1001; P < .0001), balanced crystalloid (mean difference, -964.2; 95% CI, -1492 to -436.4; P = .0001), and HES (mean difference, -1030; 95% CI, -1594 to -465.8; P = .0001) groups at the end of the experiment compared to baseline.
30451726	11	26	theme	mean	1786:1789	arg1	groups					1846:1851	NTHS (mean difference, -1668; 95% confidence interval [CI], -2336 to -1001; P < .0001), balanced crystalloid (mean difference, -964.2; 95% CI, -1492 to -436.4; P = .0001), and HES (mean difference, -1030; 95% CI, -1594 to -465.8; P = .0001) groups	1605:1851	NTHS (mean difference, -1668; 95% confidence interval [CI], -2336 to -1001; P < .0001), balanced crystalloid (mean difference, -964.2; 95% CI, -1492 to -436.4; P = .0001), and HES (mean difference, -1030; 95% CI, -1594 to -465.8; P = .0001) groups	1605:1851	Syndecan-1 significantly increased in NTHS (mean difference, -1668; 95% confidence interval [CI], -2336 to -1001; P < .0001), balanced crystalloid (mean difference, -964.2; 95% CI, -1492 to -436.4; P = .0001), and HES (mean difference, -1030; 95% CI, -1594 to -465.8; P = .0001) groups at the end of the experiment compared to baseline.
30451726	11	26	theme	mean	1786:1789	arg1	difference					1791:1800	mean difference	1786:1800	mean difference	1786:1800	Syndecan-1 significantly increased in NTHS (mean difference, -1668; 95% confidence interval [CI], -2336 to -1001; P < .0001), balanced crystalloid (mean difference, -964.2; 95% CI, -1492 to -436.4; P = .0001), and HES (mean difference, -1030; 95% CI, -1594 to -465.8; P = .0001) groups at the end of the experiment compared to baseline.
30451726	5	27	theme	30	845:846	arg1	mm					848:849	mm	848:849	mm	848:849	METHODS Fully instrumented Wistar-albino rats were subjected to a pressure-controlled NTHS (mean arterial pressure of 30 mm Hg) for 60 minutes.
30451726	15	28	theme	glycocalyx	2606:2615	arg1	degradation					2579:2589	degradation	2579:2589	degradation of endothelial glycocalyx	2579:2615	Despite degradation of endothelial glycocalyx, VBP as determined by 4 independent assays remained intact and continued to be so following fluid resuscitation.
30451726	0	29	from	Increase	71:78	arg1	Shock					108:112	Nontraumatic Hemorrhagic Shock	83:112	Nontraumatic Hemorrhagic Shock in Rats	83:120	Glycocalyx Degradation Is Independent of Vascular Barrier Permeability Increase in Nontraumatic Hemorrhagic Shock in Rats.
30451726	14	30	theme	P	2385:2385	arg1	.016					2389:2392	P = .016	2385:2392	P = .016	2385:2392	Total vessel density was altered in the normal saline (mean difference, 4.092; 95% CI, 0.6195-7.564; P = .016) and hemorrhagic shock (mean difference, 5.022; 95% CI, 1.55-8.495; P = .0024) groups compared to the control group, as well as the perfused vessel density and mean flow index.
30451726	4	31	theme	fluids	692:697	arg1	composition					677:687	the composition	673:687	the composition of fluids	673:697	We hypothesized that the shedding of the glycocalyx during NTHS has little effect on VBP and that the composition of fluids can modulate these effects.
30451726	11	32	theme	crystalloid	1702:1712	arg1	difference					1791:1800	mean difference	1786:1800	mean difference	1786:1800	Syndecan-1 significantly increased in NTHS (mean difference, -1668; 95% confidence interval [CI], -2336 to -1001; P < .0001), balanced crystalloid (mean difference, -964.2; 95% CI, -1492 to -436.4; P = .0001), and HES (mean difference, -1030; 95% CI, -1594 to -465.8; P = .0001) groups at the end of the experiment compared to baseline.
30451726	11	32	theme	crystalloid	1702:1712	arg1	groups					1846:1851	NTHS (mean difference, -1668; 95% confidence interval [CI], -2336 to -1001; P < .0001), balanced crystalloid (mean difference, -964.2; 95% CI, -1492 to -436.4; P = .0001), and HES (mean difference, -1030; 95% CI, -1594 to -465.8; P = .0001) groups	1605:1851	NTHS (mean difference, -1668; 95% confidence interval [CI], -2336 to -1001; P < .0001), balanced crystalloid (mean difference, -964.2; 95% CI, -1492 to -436.4; P = .0001), and HES (mean difference, -1030; 95% CI, -1594 to -465.8; P = .0001) groups	1605:1851	Syndecan-1 significantly increased in NTHS (mean difference, -1668; 95% confidence interval [CI], -2336 to -1001; P < .0001), balanced crystalloid (mean difference, -964.2; 95% CI, -1492 to -436.4; P = .0001), and HES (mean difference, -1030; 95% CI, -1594 to -465.8; P = .0001) groups at the end of the experiment compared to baseline.
30451726	11	33	dep	-1001	1674:1678	arg1	to					1671:1672	to	1671:1672	to	1671:1672	Syndecan-1 significantly increased in NTHS (mean difference, -1668; 95% confidence interval [CI], -2336 to -1001; P < .0001), balanced crystalloid (mean difference, -964.2; 95% CI, -1492 to -436.4; P = .0001), and HES (mean difference, -1030; 95% CI, -1594 to -465.8; P = .0001) groups at the end of the experiment compared to baseline.
30451726	11	33	dep	-1001	1674:1678	arg1	<					1683:1683	P < .0001	1681:1689	P < .0001	1681:1689	Syndecan-1 significantly increased in NTHS (mean difference, -1668; 95% confidence interval [CI], -2336 to -1001; P < .0001), balanced crystalloid (mean difference, -964.2; 95% CI, -1492 to -436.4; P = .0001), and HES (mean difference, -1030; 95% CI, -1594 to -465.8; P = .0001) groups at the end of the experiment compared to baseline.
30451726	11	34	theme	HES	1781:1783	arg1	difference					1791:1800	mean difference	1786:1800	mean difference	1786:1800	Syndecan-1 significantly increased in NTHS (mean difference, -1668; 95% confidence interval [CI], -2336 to -1001; P < .0001), balanced crystalloid (mean difference, -964.2; 95% CI, -1492 to -436.4; P = .0001), and HES (mean difference, -1030; 95% CI, -1594 to -465.8; P = .0001) groups at the end of the experiment compared to baseline.
30451726	11	34	theme	HES	1781:1783	arg1	groups					1846:1851	NTHS (mean difference, -1668; 95% confidence interval [CI], -2336 to -1001; P < .0001), balanced crystalloid (mean difference, -964.2; 95% CI, -1492 to -436.4; P = .0001), and HES (mean difference, -1030; 95% CI, -1594 to -465.8; P = .0001) groups	1605:1851	NTHS (mean difference, -1668; 95% confidence interval [CI], -2336 to -1001; P < .0001), balanced crystalloid (mean difference, -964.2; 95% CI, -1492 to -436.4; P = .0001), and HES (mean difference, -1030; 95% CI, -1594 to -465.8; P = .0001) groups	1605:1851	Syndecan-1 significantly increased in NTHS (mean difference, -1668; 95% confidence interval [CI], -2336 to -1001; P < .0001), balanced crystalloid (mean difference, -964.2; 95% CI, -1492 to -436.4; P = .0001), and HES (mean difference, -1030; 95% CI, -1594 to -465.8; P = .0001) groups at the end of the experiment compared to baseline.
30451726	9	35	theme	determination	1437:1449	arg1	decay					1297:1301	plasma decay	1290:1301	plasma decay of 3 fluorescent dyes (40- and 500-kDa dextran and 70-kDa albumin), Evans blue dye exclusion, intravital fluorescence microscopy, and determination of tissue edema (wet/dry weight ratio)	1290:1488	VBP changes were assessed using plasma decay of 3 fluorescent dyes (40- and 500-kDa dextran and 70-kDa albumin), Evans blue dye exclusion, intravital fluorescence microscopy, and determination of tissue edema (wet/dry weight ratio).
30451726	12	36	theme	=	2021:2021	arg1	P					2019:2019	P = .0001	2019:2027	P = .0001	2019:2027	Hyaluronan levels were higher at the end of the experiment in nonresuscitated NTHS (-923.1; 95% CI, -1216 to -630; P = .0001) and balanced crystalloid (-1039; 95% CI, -1332 to -745.5; P = .0001) or HES (-394.2; 95% CI, -670.1 to -118.3; P = .0027) groups compared to controls.
30451726	1	37	attach	linked	260:265	arg2	fluids					234:239	different resuscitation fluids	210:239	different resuscitation fluids	210:239	BACKGROUND Glycocalyx shedding after traumatic hemorrhagic or septic shock, as well as different resuscitation fluids, has been causally linked to increased vascular barrier permeability (VBP) resulting in tissue edema.
30451726	1	37	attach	linked	260:265	arg1	VBP					311:313	VBP	311:313	VBP	311:313	BACKGROUND Glycocalyx shedding after traumatic hemorrhagic or septic shock, as well as different resuscitation fluids, has been causally linked to increased vascular barrier permeability (VBP) resulting in tissue edema.
30451726	1	37	attach	linked	260:265	arg1	permeability					297:308	vascular barrier permeability	280:308	increased vascular barrier permeability (VBP) resulting in tissue edema	270:340	BACKGROUND Glycocalyx shedding after traumatic hemorrhagic or septic shock, as well as different resuscitation fluids, has been causally linked to increased vascular barrier permeability (VBP) resulting in tissue edema.
30451726	1	37	attach	linked	260:265	arg2	Glycocalyx					134:143	BACKGROUND Glycocalyx	123:143	BACKGROUND Glycocalyx shedding after traumatic hemorrhagic or septic shock	123:196	BACKGROUND Glycocalyx shedding after traumatic hemorrhagic or septic shock, as well as different resuscitation fluids, has been causally linked to increased vascular barrier permeability (VBP) resulting in tissue edema.
30451726	16	38	theme	microcirculation	2779:2794	arg1	alterations					2796:2806	microcirculation alterations	2779:2806	microcirculation alterations	2779:2806	CONCLUSIONS NTHS induced glycocalyx shedding and microcirculation alterations, without altering VBP.
30451726	9	39	theme	tissue	1454:1459	arg1	ratio					1483:1487	wet/dry weight ratio	1468:1487	wet/dry weight ratio	1468:1487	VBP changes were assessed using plasma decay of 3 fluorescent dyes (40- and 500-kDa dextran and 70-kDa albumin), Evans blue dye exclusion, intravital fluorescence microscopy, and determination of tissue edema (wet/dry weight ratio).
30451726	9	39	theme	tissue	1454:1459	arg1	edema					1461:1465	tissue edema	1454:1465	tissue edema (wet/dry weight ratio)	1454:1488	VBP changes were assessed using plasma decay of 3 fluorescent dyes (40- and 500-kDa dextran and 70-kDa albumin), Evans blue dye exclusion, intravital fluorescence microscopy, and determination of tissue edema (wet/dry weight ratio).
30451726	6	40	theme	%	977:977	arg1	saline					986:991	0.9% normal saline	974:991	0.9% normal saline	974:991	Animals were fluid resuscitated with Ringer's acetate, balanced hydroxyethyl starch (HES) solution, or 0.9% normal saline to a mean arterial pressure of 80 mm Hg and compared with shams or nonresuscitated NTHS.
30451726	0	41	from	Shock	108:112	arg1	Rats					117:120	Rats	117:120	Rats	117:120	Glycocalyx Degradation Is Independent of Vascular Barrier Permeability Increase in Nontraumatic Hemorrhagic Shock in Rats.
30451726	0	42	theme	Increase	71:78	arg1	Independent					26:36	Independent	26:36	Independent	26:36	Glycocalyx Degradation Is Independent of Vascular Barrier Permeability Increase in Nontraumatic Hemorrhagic Shock in Rats.
30451726	13	43	theme	microcirculation	2213:2228	arg1	alterations					2230:2240	microcirculation alterations	2213:2240	microcirculation alterations	2213:2240	Glycocalyx shedding resulted in microcirculation alterations as observed by handheld video microscopy.
30451726	18	44	theme	significant	2982:2992	arg1	contributor					2994:3004	a significant contributor	2980:3004	a significant contributor to VBP	2980:3011	These results challenge the concept that the glycocalyx barrier is a significant contributor to VBP.
30451726	18	44	theme	significant	2982:2992	arg1	barrier					2969:2975	the glycocalyx barrier	2954:2975	the glycocalyx barrier	2954:2975	These results challenge the concept that the glycocalyx barrier is a significant contributor to VBP.
30451726	11	45	theme	P	1681:1681	arg1	<					1683:1683	P < .0001	1681:1689	P < .0001	1681:1689	Syndecan-1 significantly increased in NTHS (mean difference, -1668; 95% confidence interval [CI], -2336 to -1001; P < .0001), balanced crystalloid (mean difference, -964.2; 95% CI, -1492 to -436.4; P = .0001), and HES (mean difference, -1030; 95% CI, -1594 to -465.8; P = .0001) groups at the end of the experiment compared to baseline.
30451726	0	46	theme	Nontraumatic	83:94	arg1	Shock					108:112	Nontraumatic Hemorrhagic Shock	83:112	Nontraumatic Hemorrhagic Shock in Rats	83:120	Glycocalyx Degradation Is Independent of Vascular Barrier Permeability Increase in Nontraumatic Hemorrhagic Shock in Rats.
30451726	9	47	theme	VBP	1258:1260	arg1	changes					1262:1268	VBP changes	1258:1268	VBP changes	1258:1268	VBP changes were assessed using plasma decay of 3 fluorescent dyes (40- and 500-kDa dextran and 70-kDa albumin), Evans blue dye exclusion, intravital fluorescence microscopy, and determination of tissue edema (wet/dry weight ratio).
30451726	1	48	theme	septic	185:190	arg1	shock					192:196	traumatic hemorrhagic or septic shock	160:196	shock	192:196	BACKGROUND Glycocalyx shedding after traumatic hemorrhagic or septic shock, as well as different resuscitation fluids, has been causally linked to increased vascular barrier permeability (VBP) resulting in tissue edema.
30451726	12	49	theme	%	1998:1998	arg1	CI					2000:2001	95% CI	1996:2001	95% CI	1996:2001	Hyaluronan levels were higher at the end of the experiment in nonresuscitated NTHS (-923.1; 95% CI, -1216 to -630; P = .0001) and balanced crystalloid (-1039; 95% CI, -1332 to -745.5; P = .0001) or HES (-394.2; 95% CI, -670.1 to -118.3; P = .0027) groups compared to controls.
30451726	9	50	theme	Evans	1371:1375	arg1	exclusion					1386:1394	Evans blue dye exclusion	1371:1394	Evans blue dye exclusion	1371:1394	VBP changes were assessed using plasma decay of 3 fluorescent dyes (40- and 500-kDa dextran and 70-kDa albumin), Evans blue dye exclusion, intravital fluorescence microscopy, and determination of tissue edema (wet/dry weight ratio).
30451726	0	51	theme	Vascular	41:48	arg1	Permeability					58:69	Vascular Barrier Permeability	41:69	Vascular Barrier Permeability Increase in Nontraumatic Hemorrhagic Shock in Rats	41:120	Glycocalyx Degradation Is Independent of Vascular Barrier Permeability Increase in Nontraumatic Hemorrhagic Shock in Rats.
30451726	0	52	theme	Glycocalyx	0:9	arg1	Degradation					11:21	Glycocalyx Degradation	0:21	Glycocalyx Degradation	0:21	Glycocalyx Degradation Is Independent of Vascular Barrier Permeability Increase in Nontraumatic Hemorrhagic Shock in Rats.
30451726	1	53	theme	barrier	289:295	arg1	VBP					311:313	VBP	311:313	VBP	311:313	BACKGROUND Glycocalyx shedding after traumatic hemorrhagic or septic shock, as well as different resuscitation fluids, has been causally linked to increased vascular barrier permeability (VBP) resulting in tissue edema.
30451726	1	53	theme	barrier	289:295	arg1	permeability					297:308	vascular barrier permeability	280:308	increased vascular barrier permeability (VBP) resulting in tissue edema	270:340	BACKGROUND Glycocalyx shedding after traumatic hemorrhagic or septic shock, as well as different resuscitation fluids, has been causally linked to increased vascular barrier permeability (VBP) resulting in tissue edema.
30451726	16	54	theme	CONCLUSIONS	2730:2740	arg1	NTHS					2742:2745	CONCLUSIONS NTHS	2730:2745	CONCLUSIONS NTHS	2730:2745	CONCLUSIONS NTHS induced glycocalyx shedding and microcirculation alterations, without altering VBP.
30451726	6	55	theme	arterial	1003:1010	arg1	pressure					1012:1019	a mean arterial pressure	996:1019	a mean arterial pressure of 80 mm Hg	996:1031	Animals were fluid resuscitated with Ringer's acetate, balanced hydroxyethyl starch (HES) solution, or 0.9% normal saline to a mean arterial pressure of 80 mm Hg and compared with shams or nonresuscitated NTHS.
30451726	5	56	theme	mean	819:822	arg1	pressure					833:840	mean arterial pressure	819:840	mean arterial pressure of 30 mm Hg	819:852	METHODS Fully instrumented Wistar-albino rats were subjected to a pressure-controlled NTHS (mean arterial pressure of 30 mm Hg) for 60 minutes.
30451726	14	57	theme	mean	2339:2342	arg1	saline					2331:2336	the normal saline	2320:2336	the normal saline (mean difference, 4.092; 95% CI, 0.6195-7.564; P = .016)	2320:2393	Total vessel density was altered in the normal saline (mean difference, 4.092; 95% CI, 0.6195-7.564; P = .016) and hemorrhagic shock (mean difference, 5.022; 95% CI, 1.55-8.495; P = .0024) groups compared to the control group, as well as the perfused vessel density and mean flow index.
30451726	14	57	theme	mean	2339:2342	arg1	difference					2344:2353	mean difference	2339:2353	mean difference	2339:2353	Total vessel density was altered in the normal saline (mean difference, 4.092; 95% CI, 0.6195-7.564; P = .016) and hemorrhagic shock (mean difference, 5.022; 95% CI, 1.55-8.495; P = .0024) groups compared to the control group, as well as the perfused vessel density and mean flow index.
30451726	2	58	theme	VBP	478:480	arg1	alteration					464:473	an alteration	461:473	an alteration of VBP	461:480	In nontraumatic hemorrhagic shock (NTHS), it remains questionable whether glycocalyx degradation in itself results in an alteration of VBP.
30451726	12	59	theme	HES	2102:2104	arg1	-394.2					2107:2112	-394.2	2107:2112	-394.2	2107:2112	Hyaluronan levels were higher at the end of the experiment in nonresuscitated NTHS (-923.1; 95% CI, -1216 to -630; P = .0001) and balanced crystalloid (-1039; 95% CI, -1332 to -745.5; P = .0001) or HES (-394.2; 95% CI, -670.1 to -118.3; P = .0027) groups compared to controls.
30451726	12	59	theme	HES	2102:2104	arg1	groups					2152:2157	balanced crystalloid (-1039; 95% CI, -1332 to -745.5; P = .0001) or HES (-394.2; 95% CI, -670.1 to -118.3; P = .0027) groups	2034:2157	groups	2152:2157	Hyaluronan levels were higher at the end of the experiment in nonresuscitated NTHS (-923.1; 95% CI, -1216 to -630; P = .0001) and balanced crystalloid (-1039; 95% CI, -1332 to -745.5; P = .0001) or HES (-394.2; 95% CI, -670.1 to -118.3; P = .0027) groups compared to controls.
30451726	4	60	contain	has	639:641	arg1	shedding					600:607	the shedding	596:607	the shedding of the glycocalyx during NTHS	596:637	We hypothesized that the shedding of the glycocalyx during NTHS has little effect on VBP and that the composition of fluids can modulate these effects.
30451726	4	60	contain	has	639:641	arg2	effect					650:655	little effect	643:655	little effect	643:655	We hypothesized that the shedding of the glycocalyx during NTHS has little effect on VBP and that the composition of fluids can modulate these effects.
30451726	11	61	theme	=	1767:1767	arg1	P					1765:1765	P = .0001	1765:1773	P = .0001	1765:1773	Syndecan-1 significantly increased in NTHS (mean difference, -1668; 95% confidence interval [CI], -2336 to -1001; P < .0001), balanced crystalloid (mean difference, -964.2; 95% CI, -1492 to -436.4; P = .0001), and HES (mean difference, -1030; 95% CI, -1594 to -465.8; P = .0001) groups at the end of the experiment compared to baseline.
30451726	9	62	theme	blue	1377:1380	arg1	exclusion					1386:1394	Evans blue dye exclusion	1371:1394	Evans blue dye exclusion	1371:1394	VBP changes were assessed using plasma decay of 3 fluorescent dyes (40- and 500-kDa dextran and 70-kDa albumin), Evans blue dye exclusion, intravital fluorescence microscopy, and determination of tissue edema (wet/dry weight ratio).
30451726	9	63	theme	dyes	1320:1323	arg1	decay					1297:1301	plasma decay	1290:1301	plasma decay of 3 fluorescent dyes (40- and 500-kDa dextran and 70-kDa albumin), Evans blue dye exclusion, intravital fluorescence microscopy, and determination of tissue edema (wet/dry weight ratio)	1290:1488	VBP changes were assessed using plasma decay of 3 fluorescent dyes (40- and 500-kDa dextran and 70-kDa albumin), Evans blue dye exclusion, intravital fluorescence microscopy, and determination of tissue edema (wet/dry weight ratio).
30451726	7	64	theme	shed	1093:1096	arg1	products					1098:1105	Glycocalyx shed products	1082:1105	Glycocalyx shed products	1082:1105	Glycocalyx shed products were determined at baseline and 60 minutes after fluid resuscitation.
30451726	15	65	theme	fluid	2709:2713	arg1	resuscitation					2715:2727	fluid resuscitation	2709:2727	fluid resuscitation	2709:2727	Despite degradation of endothelial glycocalyx, VBP as determined by 4 independent assays remained intact and continued to be so following fluid resuscitation.
30451726	11	66	dep	-436.4	1757:1762	arg1	to					1754:1755	to	1754:1755	to	1754:1755	Syndecan-1 significantly increased in NTHS (mean difference, -1668; 95% confidence interval [CI], -2336 to -1001; P < .0001), balanced crystalloid (mean difference, -964.2; 95% CI, -1492 to -436.4; P = .0001), and HES (mean difference, -1030; 95% CI, -1594 to -465.8; P = .0001) groups at the end of the experiment compared to baseline.
30451726	14	67	theme	saline	2331:2336	arg1	groups					2473:2478	the normal saline (mean difference, 4.092; 95% CI, 0.6195-7.564; P = .016) and hemorrhagic shock (mean difference, 5.022; 95% CI, 1.55-8.495; P = .0024) groups	2320:2478	the normal saline (mean difference, 4.092; 95% CI, 0.6195-7.564; P = .016) and hemorrhagic shock (mean difference, 5.022; 95% CI, 1.55-8.495; P = .0024) groups	2320:2478	Total vessel density was altered in the normal saline (mean difference, 4.092; 95% CI, 0.6195-7.564; P = .016) and hemorrhagic shock (mean difference, 5.022; 95% CI, 1.55-8.495; P = .0024) groups compared to the control group, as well as the perfused vessel density and mean flow index.
30451726	18	68	theme	glycocalyx	2958:2967	arg1	contributor					2994:3004	a significant contributor	2980:3004	a significant contributor to VBP	2980:3011	These results challenge the concept that the glycocalyx barrier is a significant contributor to VBP.
30451726	18	68	theme	glycocalyx	2958:2967	arg1	barrier					2969:2975	the glycocalyx barrier	2954:2975	the glycocalyx barrier	2954:2975	These results challenge the concept that the glycocalyx barrier is a significant contributor to VBP.
30451726	11	69	dep	NTHS	1605:1608	arg1	-1001					1674:1678	-1001	1674:1678	-1001	1674:1678	Syndecan-1 significantly increased in NTHS (mean difference, -1668; 95% confidence interval [CI], -2336 to -1001; P < .0001), balanced crystalloid (mean difference, -964.2; 95% CI, -1492 to -436.4; P = .0001), and HES (mean difference, -1030; 95% CI, -1594 to -465.8; P = .0001) groups at the end of the experiment compared to baseline.
30451726	13	70	theme	video	2266:2270	arg1	microscopy					2272:2281	handheld video microscopy	2257:2281	handheld video microscopy	2257:2281	Glycocalyx shedding resulted in microcirculation alterations as observed by handheld video microscopy.
30451726	2	71	theme	nontraumatic	346:357	arg1	shock					371:375	nontraumatic hemorrhagic shock	346:375	nontraumatic hemorrhagic shock (NTHS)	346:382	In nontraumatic hemorrhagic shock (NTHS), it remains questionable whether glycocalyx degradation in itself results in an alteration of VBP.
30451726	2	71	theme	nontraumatic	346:357	arg1	NTHS					378:381	NTHS	378:381	NTHS	378:381	In nontraumatic hemorrhagic shock (NTHS), it remains questionable whether glycocalyx degradation in itself results in an alteration of VBP.
30451726	0	72	theme	Barrier	50:56	arg1	Permeability					58:69	Vascular Barrier Permeability	41:69	Vascular Barrier Permeability Increase in Nontraumatic Hemorrhagic Shock in Rats	41:120	Glycocalyx Degradation Is Independent of Vascular Barrier Permeability Increase in Nontraumatic Hemorrhagic Shock in Rats.
30451726	12	73	dep	-630	2013:2016	arg1	to					2010:2011	to	2010:2011	to	2010:2011	Hyaluronan levels were higher at the end of the experiment in nonresuscitated NTHS (-923.1; 95% CI, -1216 to -630; P = .0001) and balanced crystalloid (-1039; 95% CI, -1332 to -745.5; P = .0001) or HES (-394.2; 95% CI, -670.1 to -118.3; P = .0027) groups compared to controls.
30451726	12	74	theme	=	2090:2090	arg1	P					2088:2088	P = .0001	2088:2096	P = .0001	2088:2096	Hyaluronan levels were higher at the end of the experiment in nonresuscitated NTHS (-923.1; 95% CI, -1216 to -630; P = .0001) and balanced crystalloid (-1039; 95% CI, -1332 to -745.5; P = .0001) or HES (-394.2; 95% CI, -670.1 to -118.3; P = .0027) groups compared to controls.
30451726	13	75	theme	Glycocalyx	2181:2190	arg1	shedding					2192:2199	Glycocalyx shedding	2181:2199	Glycocalyx shedding	2181:2199	Glycocalyx shedding resulted in microcirculation alterations as observed by handheld video microscopy.
30451726	9	76	theme	500-kDa	1334:1340	arg1	dextran					1342:1348	500-kDa dextran	1334:1348	500-kDa dextran	1334:1348	VBP changes were assessed using plasma decay of 3 fluorescent dyes (40- and 500-kDa dextran and 70-kDa albumin), Evans blue dye exclusion, intravital fluorescence microscopy, and determination of tissue edema (wet/dry weight ratio).
30451726	9	76	theme	500-kDa	1334:1340	arg1	dyes					1320:1323	3 fluorescent dyes	1306:1323	3 fluorescent dyes (40- and 500-kDa dextran and 70-kDa albumin)	1306:1368	VBP changes were assessed using plasma decay of 3 fluorescent dyes (40- and 500-kDa dextran and 70-kDa albumin), Evans blue dye exclusion, intravital fluorescence microscopy, and determination of tissue edema (wet/dry weight ratio).
30451726	11	77	theme	%	1812:1812	arg1	CI					1814:1815	95% CI	1810:1815	95% CI	1810:1815	Syndecan-1 significantly increased in NTHS (mean difference, -1668; 95% confidence interval [CI], -2336 to -1001; P < .0001), balanced crystalloid (mean difference, -964.2; 95% CI, -1492 to -436.4; P = .0001), and HES (mean difference, -1030; 95% CI, -1594 to -465.8; P = .0001) groups at the end of the experiment compared to baseline.
30451726	14	78	theme	flow	2559:2562	arg1	index					2564:2568	mean flow index	2554:2568	mean flow index	2554:2568	Total vessel density was altered in the normal saline (mean difference, 4.092; 95% CI, 0.6195-7.564; P = .016) and hemorrhagic shock (mean difference, 5.022; 95% CI, 1.55-8.495; P = .0024) groups compared to the control group, as well as the perfused vessel density and mean flow index.
30451726	11	79	theme	confidence	1639:1648	arg1	[CI					1659:1661	-1668; 95% confidence interval [CI	1628:1661	-1668; 95% confidence interval [CI	1628:1661	Syndecan-1 significantly increased in NTHS (mean difference, -1668; 95% confidence interval [CI], -2336 to -1001; P < .0001), balanced crystalloid (mean difference, -964.2; 95% CI, -1492 to -436.4; P = .0001), and HES (mean difference, -1030; 95% CI, -1594 to -465.8; P = .0001) groups at the end of the experiment compared to baseline.
30451726	4	80	theme	little	643:648	arg1	effect					650:655	little effect	643:655	little effect	643:655	We hypothesized that the shedding of the glycocalyx during NTHS has little effect on VBP and that the composition of fluids can modulate these effects.
30451726	2	81	from	degradation	428:438	arg1	itself					443:448	itself	443:448	itself	443:448	In nontraumatic hemorrhagic shock (NTHS), it remains questionable whether glycocalyx degradation in itself results in an alteration of VBP.
30451726	5	82	theme	Wistar-albino	754:766	arg1	rats					768:771	Wistar-albino rats	754:771	Wistar-albino rats	754:771	METHODS Fully instrumented Wistar-albino rats were subjected to a pressure-controlled NTHS (mean arterial pressure of 30 mm Hg) for 60 minutes.
30451726	3	83	theme	fluids	502:507	arg1	composition					487:497	The composition	483:497	The composition of fluids	483:507	The composition of fluids can also have a modulatory effect on glycocalyx shedding and VBP.
30451726	12	84	dep	-1039	2056:2060	arg1	CI					2067:2068	95% CI	2063:2068	95% CI	2063:2068	Hyaluronan levels were higher at the end of the experiment in nonresuscitated NTHS (-923.1; 95% CI, -1216 to -630; P = .0001) and balanced crystalloid (-1039; 95% CI, -1332 to -745.5; P = .0001) or HES (-394.2; 95% CI, -670.1 to -118.3; P = .0027) groups compared to controls.
30451726	12	84	dep	-1039	2056:2060	arg1	P					2088:2088	P = .0001	2088:2096	P = .0001	2088:2096	Hyaluronan levels were higher at the end of the experiment in nonresuscitated NTHS (-923.1; 95% CI, -1216 to -630; P = .0001) and balanced crystalloid (-1039; 95% CI, -1332 to -745.5; P = .0001) or HES (-394.2; 95% CI, -670.1 to -118.3; P = .0027) groups compared to controls.
30451726	12	84	dep	-1039	2056:2060	arg1	-745.5					2080:2085	-745.5	2080:2085	-745.5	2080:2085	Hyaluronan levels were higher at the end of the experiment in nonresuscitated NTHS (-923.1; 95% CI, -1216 to -630; P = .0001) and balanced crystalloid (-1039; 95% CI, -1332 to -745.5; P = .0001) or HES (-394.2; 95% CI, -670.1 to -118.3; P = .0027) groups compared to controls.
30451726	6	85	theme	mm	1027:1028	arg1	Hg					1030:1031	80 mm Hg	1024:1031	80 mm Hg	1024:1031	Animals were fluid resuscitated with Ringer's acetate, balanced hydroxyethyl starch (HES) solution, or 0.9% normal saline to a mean arterial pressure of 80 mm Hg and compared with shams or nonresuscitated NTHS.
30451726	12	86	theme	=	2143:2143	arg1	P					2141:2141	P = .0027	2141:2149	P = .0027	2141:2149	Hyaluronan levels were higher at the end of the experiment in nonresuscitated NTHS (-923.1; 95% CI, -1216 to -630; P = .0001) and balanced crystalloid (-1039; 95% CI, -1332 to -745.5; P = .0001) or HES (-394.2; 95% CI, -670.1 to -118.3; P = .0027) groups compared to controls.
30451726	14	87	dep	saline	2331:2336	arg1	=					2464:2464	=	2464:2464	=	2464:2464	Total vessel density was altered in the normal saline (mean difference, 4.092; 95% CI, 0.6195-7.564; P = .016) and hemorrhagic shock (mean difference, 5.022; 95% CI, 1.55-8.495; P = .0024) groups compared to the control group, as well as the perfused vessel density and mean flow index.
30451726	1	88	theme	increased	270:278	arg1	VBP					311:313	VBP	311:313	VBP	311:313	BACKGROUND Glycocalyx shedding after traumatic hemorrhagic or septic shock, as well as different resuscitation fluids, has been causally linked to increased vascular barrier permeability (VBP) resulting in tissue edema.
30451726	1	88	theme	increased	270:278	arg1	permeability					297:308	vascular barrier permeability	280:308	increased vascular barrier permeability (VBP) resulting in tissue edema	270:340	BACKGROUND Glycocalyx shedding after traumatic hemorrhagic or septic shock, as well as different resuscitation fluids, has been causally linked to increased vascular barrier permeability (VBP) resulting in tissue edema.
30451726	5	89	dep	NTHS	813:816	arg1	pressure					833:840	mean arterial pressure	819:840	mean arterial pressure of 30 mm Hg	819:852	METHODS Fully instrumented Wistar-albino rats were subjected to a pressure-controlled NTHS (mean arterial pressure of 30 mm Hg) for 60 minutes.
30451726	11	90	dep	-465.8	1827:1832	arg1	to					1824:1825	to	1824:1825	to	1824:1825	Syndecan-1 significantly increased in NTHS (mean difference, -1668; 95% confidence interval [CI], -2336 to -1001; P < .0001), balanced crystalloid (mean difference, -964.2; 95% CI, -1492 to -436.4; P = .0001), and HES (mean difference, -1030; 95% CI, -1594 to -465.8; P = .0001) groups at the end of the experiment compared to baseline.
30451726	6	91	dep	resuscitated	890:901	arg1	fluid					884:888	fluid	884:888	fluid	884:888	Animals were fluid resuscitated with Ringer's acetate, balanced hydroxyethyl starch (HES) solution, or 0.9% normal saline to a mean arterial pressure of 80 mm Hg and compared with shams or nonresuscitated NTHS.
30451726	14	92	theme	normal	2324:2329	arg1	saline					2331:2336	the normal saline	2320:2336	the normal saline (mean difference, 4.092; 95% CI, 0.6195-7.564; P = .016)	2320:2393	Total vessel density was altered in the normal saline (mean difference, 4.092; 95% CI, 0.6195-7.564; P = .016) and hemorrhagic shock (mean difference, 5.022; 95% CI, 1.55-8.495; P = .0024) groups compared to the control group, as well as the perfused vessel density and mean flow index.
30451726	14	92	theme	normal	2324:2329	arg1	difference					2344:2353	mean difference	2339:2353	mean difference	2339:2353	Total vessel density was altered in the normal saline (mean difference, 4.092; 95% CI, 0.6195-7.564; P = .016) and hemorrhagic shock (mean difference, 5.022; 95% CI, 1.55-8.495; P = .0024) groups compared to the control group, as well as the perfused vessel density and mean flow index.
30451726	14	93	dep	difference	2344:2353	arg1	4.092					2356:2360	4.092	2356:2360	4.092	2356:2360	Total vessel density was altered in the normal saline (mean difference, 4.092; 95% CI, 0.6195-7.564; P = .016) and hemorrhagic shock (mean difference, 5.022; 95% CI, 1.55-8.495; P = .0024) groups compared to the control group, as well as the perfused vessel density and mean flow index.
30451726	14	93	dep	difference	2344:2353	arg1	0.6195-7.564					2371:2382	0.6195-7.564	2371:2382	0.6195-7.564	2371:2382	Total vessel density was altered in the normal saline (mean difference, 4.092; 95% CI, 0.6195-7.564; P = .016) and hemorrhagic shock (mean difference, 5.022; 95% CI, 1.55-8.495; P = .0024) groups compared to the control group, as well as the perfused vessel density and mean flow index.
30451726	14	93	dep	difference	2344:2353	arg1	CI					2367:2368	95% CI	2363:2368	95% CI	2363:2368	Total vessel density was altered in the normal saline (mean difference, 4.092; 95% CI, 0.6195-7.564; P = .016) and hemorrhagic shock (mean difference, 5.022; 95% CI, 1.55-8.495; P = .0024) groups compared to the control group, as well as the perfused vessel density and mean flow index.
30451726	14	93	dep	difference	2344:2353	arg1	.016					2389:2392	P = .016	2385:2392	P = .016	2385:2392	Total vessel density was altered in the normal saline (mean difference, 4.092; 95% CI, 0.6195-7.564; P = .016) and hemorrhagic shock (mean difference, 5.022; 95% CI, 1.55-8.495; P = .0024) groups compared to the control group, as well as the perfused vessel density and mean flow index.
30451726	5	94	theme	mm	848:849	arg1	Hg					851:852	30 mm Hg	845:852	30 mm Hg	845:852	METHODS Fully instrumented Wistar-albino rats were subjected to a pressure-controlled NTHS (mean arterial pressure of 30 mm Hg) for 60 minutes.
30451726	11	95	dep	difference	1791:1800	arg1	CI					1814:1815	95% CI	1810:1815	95% CI	1810:1815	Syndecan-1 significantly increased in NTHS (mean difference, -1668; 95% confidence interval [CI], -2336 to -1001; P < .0001), balanced crystalloid (mean difference, -964.2; 95% CI, -1492 to -436.4; P = .0001), and HES (mean difference, -1030; 95% CI, -1594 to -465.8; P = .0001) groups at the end of the experiment compared to baseline.
30451726	11	95	dep	difference	1791:1800	arg1	-465.8					1827:1832	-465.8	1827:1832	-465.8	1827:1832	Syndecan-1 significantly increased in NTHS (mean difference, -1668; 95% confidence interval [CI], -2336 to -1001; P < .0001), balanced crystalloid (mean difference, -964.2; 95% CI, -1492 to -436.4; P = .0001), and HES (mean difference, -1030; 95% CI, -1594 to -465.8; P = .0001) groups at the end of the experiment compared to baseline.
30451726	11	95	dep	difference	1791:1800	arg1	P					1835:1835	P = .0001	1835:1843	P = .0001	1835:1843	Syndecan-1 significantly increased in NTHS (mean difference, -1668; 95% confidence interval [CI], -2336 to -1001; P < .0001), balanced crystalloid (mean difference, -964.2; 95% CI, -1492 to -436.4; P = .0001), and HES (mean difference, -1030; 95% CI, -1594 to -465.8; P = .0001) groups at the end of the experiment compared to baseline.
30451726	11	95	dep	difference	1791:1800	arg1	-1030					1803:1807	-1030	1803:1807	-1030	1803:1807	Syndecan-1 significantly increased in NTHS (mean difference, -1668; 95% confidence interval [CI], -2336 to -1001; P < .0001), balanced crystalloid (mean difference, -964.2; 95% CI, -1492 to -436.4; P = .0001), and HES (mean difference, -1030; 95% CI, -1594 to -465.8; P = .0001) groups at the end of the experiment compared to baseline.
30451726	8	96	theme	Skeletal	1177:1184	arg1	microcirculation					1193:1208	Skeletal muscle microcirculation	1177:1208	Skeletal muscle microcirculation	1177:1208	Skeletal muscle microcirculation was visualized using handheld vital microscopy.
30451726	7	97	theme	fluid	1156:1160	arg1	resuscitation					1162:1174	fluid resuscitation	1156:1174	fluid resuscitation	1156:1174	Glycocalyx shed products were determined at baseline and 60 minutes after fluid resuscitation.
30451726	9	98	theme	exclusion	1386:1394	arg1	decay					1297:1301	plasma decay	1290:1301	plasma decay of 3 fluorescent dyes (40- and 500-kDa dextran and 70-kDa albumin), Evans blue dye exclusion, intravital fluorescence microscopy, and determination of tissue edema (wet/dry weight ratio)	1290:1488	VBP changes were assessed using plasma decay of 3 fluorescent dyes (40- and 500-kDa dextran and 70-kDa albumin), Evans blue dye exclusion, intravital fluorescence microscopy, and determination of tissue edema (wet/dry weight ratio).
30451726	14	99	theme	hemorrhagic	2399:2409	arg1	shock					2411:2415	hemorrhagic shock	2399:2415	hemorrhagic shock	2399:2415	Total vessel density was altered in the normal saline (mean difference, 4.092; 95% CI, 0.6195-7.564; P = .016) and hemorrhagic shock (mean difference, 5.022; 95% CI, 1.55-8.495; P = .0024) groups compared to the control group, as well as the perfused vessel density and mean flow index.
30451726	11	100	theme	balanced	1693:1700	arg1	crystalloid					1702:1712	balanced crystalloid	1693:1712	balanced crystalloid (mean difference, -964.2; 95% CI, -1492 to -436.4; P = .0001)	1693:1774	Syndecan-1 significantly increased in NTHS (mean difference, -1668; 95% confidence interval [CI], -2336 to -1001; P < .0001), balanced crystalloid (mean difference, -964.2; 95% CI, -1492 to -436.4; P = .0001), and HES (mean difference, -1030; 95% CI, -1594 to -465.8; P = .0001) groups at the end of the experiment compared to baseline.
30451726	11	100	theme	balanced	1693:1700	arg1	difference					1720:1729	mean difference	1715:1729	mean difference	1715:1729	Syndecan-1 significantly increased in NTHS (mean difference, -1668; 95% confidence interval [CI], -2336 to -1001; P < .0001), balanced crystalloid (mean difference, -964.2; 95% CI, -1492 to -436.4; P = .0001), and HES (mean difference, -1030; 95% CI, -1594 to -465.8; P = .0001) groups at the end of the experiment compared to baseline.
30451726	3	101	theme	glycocalyx	546:555	arg1	shedding					557:564	glycocalyx shedding	546:564	glycocalyx shedding	546:564	The composition of fluids can also have a modulatory effect on glycocalyx shedding and VBP.
30451726	9	102	theme	intravital	1397:1406	arg1	microscopy					1421:1430	intravital fluorescence microscopy	1397:1430	intravital fluorescence microscopy	1397:1430	VBP changes were assessed using plasma decay of 3 fluorescent dyes (40- and 500-kDa dextran and 70-kDa albumin), Evans blue dye exclusion, intravital fluorescence microscopy, and determination of tissue edema (wet/dry weight ratio).
30451726	6	103	theme	hydroxyethyl	935:946	arg1	HES					956:958	HES	956:958	HES	956:958	Animals were fluid resuscitated with Ringer's acetate, balanced hydroxyethyl starch (HES) solution, or 0.9% normal saline to a mean arterial pressure of 80 mm Hg and compared with shams or nonresuscitated NTHS.
30451726	6	103	theme	hydroxyethyl	935:946	arg1	starch					948:953	hydroxyethyl starch	935:953	balanced hydroxyethyl starch (HES) solution	926:968	Animals were fluid resuscitated with Ringer's acetate, balanced hydroxyethyl starch (HES) solution, or 0.9% normal saline to a mean arterial pressure of 80 mm Hg and compared with shams or nonresuscitated NTHS.
30451726	9	104	theme	weight	1476:1481	arg1	ratio					1483:1487	wet/dry weight ratio	1468:1487	wet/dry weight ratio	1468:1487	VBP changes were assessed using plasma decay of 3 fluorescent dyes (40- and 500-kDa dextran and 70-kDa albumin), Evans blue dye exclusion, intravital fluorescence microscopy, and determination of tissue edema (wet/dry weight ratio).
30451726	9	104	theme	weight	1476:1481	arg1	edema					1461:1465	tissue edema	1454:1465	tissue edema (wet/dry weight ratio)	1454:1488	VBP changes were assessed using plasma decay of 3 fluorescent dyes (40- and 500-kDa dextran and 70-kDa albumin), Evans blue dye exclusion, intravital fluorescence microscopy, and determination of tissue edema (wet/dry weight ratio).
30451726	12	105	theme	%	2117:2117	arg1	CI					2119:2120	95% CI	2115:2120	95% CI	2115:2120	Hyaluronan levels were higher at the end of the experiment in nonresuscitated NTHS (-923.1; 95% CI, -1216 to -630; P = .0001) and balanced crystalloid (-1039; 95% CI, -1332 to -745.5; P = .0001) or HES (-394.2; 95% CI, -670.1 to -118.3; P = .0027) groups compared to controls.
30451726	4	106	theme	glycocalyx	616:625	arg1	shedding					600:607	the shedding	596:607	the shedding of the glycocalyx during NTHS	596:637	We hypothesized that the shedding of the glycocalyx during NTHS has little effect on VBP and that the composition of fluids can modulate these effects.
30451726	12	107	dep	-923.1	1988:1993	arg1	-630					2013:2016	-630	2013:2016	-630	2013:2016	Hyaluronan levels were higher at the end of the experiment in nonresuscitated NTHS (-923.1; 95% CI, -1216 to -630; P = .0001) and balanced crystalloid (-1039; 95% CI, -1332 to -745.5; P = .0001) or HES (-394.2; 95% CI, -670.1 to -118.3; P = .0027) groups compared to controls.
30451726	12	107	dep	-923.1	1988:1993	arg1	CI					2000:2001	95% CI	1996:2001	95% CI	1996:2001	Hyaluronan levels were higher at the end of the experiment in nonresuscitated NTHS (-923.1; 95% CI, -1216 to -630; P = .0001) and balanced crystalloid (-1039; 95% CI, -1332 to -745.5; P = .0001) or HES (-394.2; 95% CI, -670.1 to -118.3; P = .0027) groups compared to controls.
30451726	12	107	dep	-923.1	1988:1993	arg1	P					2019:2019	P = .0001	2019:2027	P = .0001	2019:2027	Hyaluronan levels were higher at the end of the experiment in nonresuscitated NTHS (-923.1; 95% CI, -1216 to -630; P = .0001) and balanced crystalloid (-1039; 95% CI, -1332 to -745.5; P = .0001) or HES (-394.2; 95% CI, -670.1 to -118.3; P = .0027) groups compared to controls.
30451726	1	108	theme	resuscitation	220:232	arg1	fluids					234:239	different resuscitation fluids	210:239	different resuscitation fluids	210:239	BACKGROUND Glycocalyx shedding after traumatic hemorrhagic or septic shock, as well as different resuscitation fluids, has been causally linked to increased vascular barrier permeability (VBP) resulting in tissue edema.
30451726	15	109	theme	endothelial	2594:2604	arg1	glycocalyx					2606:2615	endothelial glycocalyx	2594:2615	endothelial glycocalyx	2594:2615	Despite degradation of endothelial glycocalyx, VBP as determined by 4 independent assays remained intact and continued to be so following fluid resuscitation.
30451726	11	110	theme	=	1837:1837	arg1	P					1835:1835	P = .0001	1835:1843	P = .0001	1835:1843	Syndecan-1 significantly increased in NTHS (mean difference, -1668; 95% confidence interval [CI], -2336 to -1001; P < .0001), balanced crystalloid (mean difference, -964.2; 95% CI, -1492 to -436.4; P = .0001), and HES (mean difference, -1030; 95% CI, -1594 to -465.8; P = .0001) groups at the end of the experiment compared to baseline.
30451726	14	111	theme	=	2387:2387	arg1	.016					2389:2392	P = .016	2385:2392	P = .016	2385:2392	Total vessel density was altered in the normal saline (mean difference, 4.092; 95% CI, 0.6195-7.564; P = .016) and hemorrhagic shock (mean difference, 5.022; 95% CI, 1.55-8.495; P = .0024) groups compared to the control group, as well as the perfused vessel density and mean flow index.
30451726	8	112	theme	handheld	1231:1238	arg1	microscopy					1246:1255	handheld vital microscopy	1231:1255	handheld vital microscopy	1231:1255	Skeletal muscle microcirculation was visualized using handheld vital microscopy.
30451726	14	113	theme	%	2444:2444	arg1	CI					2446:2447	95% CI	2442:2447	95% CI	2442:2447	Total vessel density was altered in the normal saline (mean difference, 4.092; 95% CI, 0.6195-7.564; P = .016) and hemorrhagic shock (mean difference, 5.022; 95% CI, 1.55-8.495; P = .0024) groups compared to the control group, as well as the perfused vessel density and mean flow index.
30451726	12	114	theme	%	2065:2065	arg1	CI					2067:2068	95% CI	2063:2068	95% CI	2063:2068	Hyaluronan levels were higher at the end of the experiment in nonresuscitated NTHS (-923.1; 95% CI, -1216 to -630; P = .0001) and balanced crystalloid (-1039; 95% CI, -1332 to -745.5; P = .0001) or HES (-394.2; 95% CI, -670.1 to -118.3; P = .0027) groups compared to controls.
30451726	1	115	theme	BACKGROUND	123:132	arg1	Glycocalyx					134:143	BACKGROUND Glycocalyx	123:143	BACKGROUND Glycocalyx shedding after traumatic hemorrhagic or septic shock	123:196	BACKGROUND Glycocalyx shedding after traumatic hemorrhagic or septic shock, as well as different resuscitation fluids, has been causally linked to increased vascular barrier permeability (VBP) resulting in tissue edema.
30451726	10	116	dep	RESULTS	1491:1497	arg1	upgraded					1537:1544	upgraded	1537:1544	were upgraded as a result of NTHS	1532:1564	RESULTS All glycocalyx shedding products were upgraded as a result of NTHS.
30451726	12	117	dep	-394.2	2107:2112	arg1	P					2141:2141	P = .0027	2141:2149	P = .0027	2141:2149	Hyaluronan levels were higher at the end of the experiment in nonresuscitated NTHS (-923.1; 95% CI, -1216 to -630; P = .0001) and balanced crystalloid (-1039; 95% CI, -1332 to -745.5; P = .0001) or HES (-394.2; 95% CI, -670.1 to -118.3; P = .0027) groups compared to controls.
30451726	12	117	dep	-394.2	2107:2112	arg1	-118.3					2133:2138	-118.3	2133:2138	-118.3	2133:2138	Hyaluronan levels were higher at the end of the experiment in nonresuscitated NTHS (-923.1; 95% CI, -1216 to -630; P = .0001) and balanced crystalloid (-1039; 95% CI, -1332 to -745.5; P = .0001) or HES (-394.2; 95% CI, -670.1 to -118.3; P = .0027) groups compared to controls.
30451726	12	117	dep	-394.2	2107:2112	arg1	CI					2119:2120	95% CI	2115:2120	95% CI	2115:2120	Hyaluronan levels were higher at the end of the experiment in nonresuscitated NTHS (-923.1; 95% CI, -1216 to -630; P = .0001) and balanced crystalloid (-1039; 95% CI, -1332 to -745.5; P = .0001) or HES (-394.2; 95% CI, -670.1 to -118.3; P = .0027) groups compared to controls.
30451726	6	118	theme	normal	979:984	arg1	saline					986:991	0.9% normal saline	974:991	0.9% normal saline	974:991	Animals were fluid resuscitated with Ringer's acetate, balanced hydroxyethyl starch (HES) solution, or 0.9% normal saline to a mean arterial pressure of 80 mm Hg and compared with shams or nonresuscitated NTHS.
30451726	13	119	theme	handheld	2257:2264	arg1	microscopy					2272:2281	handheld video microscopy	2257:2281	handheld video microscopy	2257:2281	Glycocalyx shedding resulted in microcirculation alterations as observed by handheld video microscopy.
30451726	5	120	theme	arterial	824:831	arg1	pressure					833:840	mean arterial pressure	819:840	mean arterial pressure of 30 mm Hg	819:852	METHODS Fully instrumented Wistar-albino rats were subjected to a pressure-controlled NTHS (mean arterial pressure of 30 mm Hg) for 60 minutes.
30451726	9	121	theme	edema	1461:1465	arg1	dyes					1320:1323	3 fluorescent dyes	1306:1323	3 fluorescent dyes (40- and 500-kDa dextran and 70-kDa albumin)	1306:1368	VBP changes were assessed using plasma decay of 3 fluorescent dyes (40- and 500-kDa dextran and 70-kDa albumin), Evans blue dye exclusion, intravital fluorescence microscopy, and determination of tissue edema (wet/dry weight ratio).
30451726	9	121	theme	edema	1461:1465	arg1	exclusion					1386:1394	Evans blue dye exclusion	1371:1394	Evans blue dye exclusion	1371:1394	VBP changes were assessed using plasma decay of 3 fluorescent dyes (40- and 500-kDa dextran and 70-kDa albumin), Evans blue dye exclusion, intravital fluorescence microscopy, and determination of tissue edema (wet/dry weight ratio).
30451726	9	121	theme	edema	1461:1465	arg1	determination					1437:1449	determination	1437:1449	determination of tissue edema (wet/dry weight ratio)	1437:1488	VBP changes were assessed using plasma decay of 3 fluorescent dyes (40- and 500-kDa dextran and 70-kDa albumin), Evans blue dye exclusion, intravital fluorescence microscopy, and determination of tissue edema (wet/dry weight ratio).
30451726	9	121	theme	edema	1461:1465	arg1	albumin					1361:1367	70-kDa albumin	1354:1367	70-kDa albumin	1354:1367	VBP changes were assessed using plasma decay of 3 fluorescent dyes (40- and 500-kDa dextran and 70-kDa albumin), Evans blue dye exclusion, intravital fluorescence microscopy, and determination of tissue edema (wet/dry weight ratio).
30451726	9	121	theme	edema	1461:1465	arg1	microscopy					1421:1430	intravital fluorescence microscopy	1397:1430	intravital fluorescence microscopy	1397:1430	VBP changes were assessed using plasma decay of 3 fluorescent dyes (40- and 500-kDa dextran and 70-kDa albumin), Evans blue dye exclusion, intravital fluorescence microscopy, and determination of tissue edema (wet/dry weight ratio).
30451726	9	121	theme	edema	1461:1465	arg1	dextran					1342:1348	500-kDa dextran	1334:1348	500-kDa dextran	1334:1348	VBP changes were assessed using plasma decay of 3 fluorescent dyes (40- and 500-kDa dextran and 70-kDa albumin), Evans blue dye exclusion, intravital fluorescence microscopy, and determination of tissue edema (wet/dry weight ratio).
30451726	6	122	theme	0.9	974:976	arg1	%					977:977	%	977:977	%	977:977	Animals were fluid resuscitated with Ringer's acetate, balanced hydroxyethyl starch (HES) solution, or 0.9% normal saline to a mean arterial pressure of 80 mm Hg and compared with shams or nonresuscitated NTHS.
30451726	14	123	theme	%	2365:2365	arg1	CI					2367:2368	95% CI	2363:2368	95% CI	2363:2368	Total vessel density was altered in the normal saline (mean difference, 4.092; 95% CI, 0.6195-7.564; P = .016) and hemorrhagic shock (mean difference, 5.022; 95% CI, 1.55-8.495; P = .0024) groups compared to the control group, as well as the perfused vessel density and mean flow index.
30451726	0	124	theme	Hemorrhagic	96:106	arg1	Shock					108:112	Nontraumatic Hemorrhagic Shock	83:112	Nontraumatic Hemorrhagic Shock in Rats	83:120	Glycocalyx Degradation Is Independent of Vascular Barrier Permeability Increase in Nontraumatic Hemorrhagic Shock in Rats.
30451726	15	125	dep	VBP	2618:2620	arg1	determined					2625:2634	determined	2625:2634	determined by 4 independent assays	2625:2658	Despite degradation of endothelial glycocalyx, VBP as determined by 4 independent assays remained intact and continued to be so following fluid resuscitation.
30451726	14	126	dep	=	2464:2464	arg1	difference					2423:2432	mean difference	2418:2432	mean difference	2418:2432	Total vessel density was altered in the normal saline (mean difference, 4.092; 95% CI, 0.6195-7.564; P = .016) and hemorrhagic shock (mean difference, 5.022; 95% CI, 1.55-8.495; P = .0024) groups compared to the control group, as well as the perfused vessel density and mean flow index.
30451726	14	126	dep	=	2464:2464	arg1	5.022					2435:2439	5.022	2435:2439	5.022	2435:2439	Total vessel density was altered in the normal saline (mean difference, 4.092; 95% CI, 0.6195-7.564; P = .016) and hemorrhagic shock (mean difference, 5.022; 95% CI, 1.55-8.495; P = .0024) groups compared to the control group, as well as the perfused vessel density and mean flow index.
30451726	14	126	dep	=	2464:2464	arg1	1.55-8.495					2450:2459	1.55-8.495	2450:2459	1.55-8.495	2450:2459	Total vessel density was altered in the normal saline (mean difference, 4.092; 95% CI, 0.6195-7.564; P = .016) and hemorrhagic shock (mean difference, 5.022; 95% CI, 1.55-8.495; P = .0024) groups compared to the control group, as well as the perfused vessel density and mean flow index.
30451726	14	126	dep	=	2464:2464	arg1	CI					2446:2447	95% CI	2442:2447	95% CI	2442:2447	Total vessel density was altered in the normal saline (mean difference, 4.092; 95% CI, 0.6195-7.564; P = .016) and hemorrhagic shock (mean difference, 5.022; 95% CI, 1.55-8.495; P = .0024) groups compared to the control group, as well as the perfused vessel density and mean flow index.
30451726	9	127	theme	wet/dry	1468:1474	arg1	ratio					1483:1487	wet/dry weight ratio	1468:1487	wet/dry weight ratio	1468:1487	VBP changes were assessed using plasma decay of 3 fluorescent dyes (40- and 500-kDa dextran and 70-kDa albumin), Evans blue dye exclusion, intravital fluorescence microscopy, and determination of tissue edema (wet/dry weight ratio).
30451726	9	127	theme	wet/dry	1468:1474	arg1	edema					1461:1465	tissue edema	1454:1465	tissue edema (wet/dry weight ratio)	1454:1488	VBP changes were assessed using plasma decay of 3 fluorescent dyes (40- and 500-kDa dextran and 70-kDa albumin), Evans blue dye exclusion, intravital fluorescence microscopy, and determination of tissue edema (wet/dry weight ratio).
30451726	15	128	theme	independent	2641:2651	arg1	assays					2653:2658	4 independent assays	2639:2658	4 independent assays	2639:2658	Despite degradation of endothelial glycocalyx, VBP as determined by 4 independent assays remained intact and continued to be so following fluid resuscitation.
30451726	3	129	contain	have	518:521	arg1	composition					487:497	The composition	483:497	The composition of fluids	483:507	The composition of fluids can also have a modulatory effect on glycocalyx shedding and VBP.
30451726	3	129	contain	have	518:521	arg2	effect					536:541	a modulatory effect	523:541	a modulatory effect	523:541	The composition of fluids can also have a modulatory effect on glycocalyx shedding and VBP.
30451726	14	130	theme	mean	2554:2557	arg1	index					2564:2568	mean flow index	2554:2568	mean flow index	2554:2568	Total vessel density was altered in the normal saline (mean difference, 4.092; 95% CI, 0.6195-7.564; P = .016) and hemorrhagic shock (mean difference, 5.022; 95% CI, 1.55-8.495; P = .0024) groups compared to the control group, as well as the perfused vessel density and mean flow index.
30451726	1	131	theme	different	210:218	arg1	fluids					234:239	different resuscitation fluids	210:239	different resuscitation fluids	210:239	BACKGROUND Glycocalyx shedding after traumatic hemorrhagic or septic shock, as well as different resuscitation fluids, has been causally linked to increased vascular barrier permeability (VBP) resulting in tissue edema.
30451726	6	132	theme	80	1024:1025	arg1	mm					1027:1028	mm	1027:1028	mm	1027:1028	Animals were fluid resuscitated with Ringer's acetate, balanced hydroxyethyl starch (HES) solution, or 0.9% normal saline to a mean arterial pressure of 80 mm Hg and compared with shams or nonresuscitated NTHS.
30451726	9	133	dep	dyes	1320:1323	arg1	40-					1326:1328	40-	1326:1328	40-	1326:1328	VBP changes were assessed using plasma decay of 3 fluorescent dyes (40- and 500-kDa dextran and 70-kDa albumin), Evans blue dye exclusion, intravital fluorescence microscopy, and determination of tissue edema (wet/dry weight ratio).
30451726	9	133	dep	dyes	1320:1323	arg1	dextran					1342:1348	500-kDa dextran	1334:1348	500-kDa dextran	1334:1348	VBP changes were assessed using plasma decay of 3 fluorescent dyes (40- and 500-kDa dextran and 70-kDa albumin), Evans blue dye exclusion, intravital fluorescence microscopy, and determination of tissue edema (wet/dry weight ratio).
30451726	9	133	dep	dyes	1320:1323	arg1	dyes					1320:1323	3 fluorescent dyes	1306:1323	3 fluorescent dyes (40- and 500-kDa dextran and 70-kDa albumin)	1306:1368	VBP changes were assessed using plasma decay of 3 fluorescent dyes (40- and 500-kDa dextran and 70-kDa albumin), Evans blue dye exclusion, intravital fluorescence microscopy, and determination of tissue edema (wet/dry weight ratio).
30451726	5	134	theme	pressure-controlled	793:811	arg1	NTHS					813:816	a pressure-controlled NTHS	791:816	a pressure-controlled NTHS (mean arterial pressure of 30 mm Hg) for 60 minutes	791:868	METHODS Fully instrumented Wistar-albino rats were subjected to a pressure-controlled NTHS (mean arterial pressure of 30 mm Hg) for 60 minutes.
30451726	9	135	theme	plasma	1290:1295	arg1	decay					1297:1301	plasma decay	1290:1301	plasma decay of 3 fluorescent dyes (40- and 500-kDa dextran and 70-kDa albumin), Evans blue dye exclusion, intravital fluorescence microscopy, and determination of tissue edema (wet/dry weight ratio)	1290:1488	VBP changes were assessed using plasma decay of 3 fluorescent dyes (40- and 500-kDa dextran and 70-kDa albumin), Evans blue dye exclusion, intravital fluorescence microscopy, and determination of tissue edema (wet/dry weight ratio).
30451726	12	136	theme	experiment	1952:1961	arg1	end					1941:1943	the end	1937:1943	the end of the experiment	1937:1961	Hyaluronan levels were higher at the end of the experiment in nonresuscitated NTHS (-923.1; 95% CI, -1216 to -630; P = .0001) and balanced crystalloid (-1039; 95% CI, -1332 to -745.5; P = .0001) or HES (-394.2; 95% CI, -670.1 to -118.3; P = .0027) groups compared to controls.
30451726	17	137	theme	Fluid	2831:2835	arg1	resuscitation					2837:2849	Fluid resuscitation	2831:2849	Fluid resuscitation	2831:2849	Fluid resuscitation partially restored the microcirculation without altering VBP.
30451726	12	138	dep	-118.3	2133:2138	arg1	to					2130:2131	to	2130:2131	to	2130:2131	Hyaluronan levels were higher at the end of the experiment in nonresuscitated NTHS (-923.1; 95% CI, -1216 to -630; P = .0001) and balanced crystalloid (-1039; 95% CI, -1332 to -745.5; P = .0001) or HES (-394.2; 95% CI, -670.1 to -118.3; P = .0027) groups compared to controls.
30451726	6	139	theme	mean	998:1001	arg1	pressure					1012:1019	a mean arterial pressure	996:1019	a mean arterial pressure of 80 mm Hg	996:1031	Animals were fluid resuscitated with Ringer's acetate, balanced hydroxyethyl starch (HES) solution, or 0.9% normal saline to a mean arterial pressure of 80 mm Hg and compared with shams or nonresuscitated NTHS.
30451726	12	140	theme	nonresuscitated	1966:1980	arg1	NTHS					1982:1985	nonresuscitated NTHS	1966:1985	nonresuscitated NTHS	1966:1985	Hyaluronan levels were higher at the end of the experiment in nonresuscitated NTHS (-923.1; 95% CI, -1216 to -630; P = .0001) and balanced crystalloid (-1039; 95% CI, -1332 to -745.5; P = .0001) or HES (-394.2; 95% CI, -670.1 to -118.3; P = .0027) groups compared to controls.
30451726	2	141	theme	glycocalyx	417:426	arg1	degradation					428:438	glycocalyx degradation	417:438	glycocalyx degradation in itself	417:448	In nontraumatic hemorrhagic shock (NTHS), it remains questionable whether glycocalyx degradation in itself results in an alteration of VBP.
30451726	7	142	theme	Glycocalyx	1082:1091	arg1	products					1098:1105	Glycocalyx shed products	1082:1105	Glycocalyx shed products	1082:1105	Glycocalyx shed products were determined at baseline and 60 minutes after fluid resuscitation.
30451726	9	143	theme	fluorescent	1308:1318	arg1	albumin					1361:1367	70-kDa albumin	1354:1367	70-kDa albumin	1354:1367	VBP changes were assessed using plasma decay of 3 fluorescent dyes (40- and 500-kDa dextran and 70-kDa albumin), Evans blue dye exclusion, intravital fluorescence microscopy, and determination of tissue edema (wet/dry weight ratio).
30451726	9	143	theme	fluorescent	1308:1318	arg1	dextran					1342:1348	500-kDa dextran	1334:1348	500-kDa dextran	1334:1348	VBP changes were assessed using plasma decay of 3 fluorescent dyes (40- and 500-kDa dextran and 70-kDa albumin), Evans blue dye exclusion, intravital fluorescence microscopy, and determination of tissue edema (wet/dry weight ratio).
30451726	9	143	theme	fluorescent	1308:1318	arg1	dyes					1320:1323	3 fluorescent dyes	1306:1323	3 fluorescent dyes (40- and 500-kDa dextran and 70-kDa albumin)	1306:1368	VBP changes were assessed using plasma decay of 3 fluorescent dyes (40- and 500-kDa dextran and 70-kDa albumin), Evans blue dye exclusion, intravital fluorescence microscopy, and determination of tissue edema (wet/dry weight ratio).
30451726	9	144	theme	microscopy	1421:1430	arg1	decay					1297:1301	plasma decay	1290:1301	plasma decay of 3 fluorescent dyes (40- and 500-kDa dextran and 70-kDa albumin), Evans blue dye exclusion, intravital fluorescence microscopy, and determination of tissue edema (wet/dry weight ratio)	1290:1488	VBP changes were assessed using plasma decay of 3 fluorescent dyes (40- and 500-kDa dextran and 70-kDa albumin), Evans blue dye exclusion, intravital fluorescence microscopy, and determination of tissue edema (wet/dry weight ratio).
30451726	14	145	theme	vessel	2535:2540	arg1	density					2542:2548	the perfused vessel density	2522:2548	the perfused vessel density	2522:2548	Total vessel density was altered in the normal saline (mean difference, 4.092; 95% CI, 0.6195-7.564; P = .016) and hemorrhagic shock (mean difference, 5.022; 95% CI, 1.55-8.495; P = .0024) groups compared to the control group, as well as the perfused vessel density and mean flow index.
30451726	0	146	theme	Permeability	58:69	arg1	Increase					71:78	Vascular Barrier Permeability Increase	41:78	Vascular Barrier Permeability Increase in Nontraumatic Hemorrhagic Shock in Rats	41:120	Glycocalyx Degradation Is Independent of Vascular Barrier Permeability Increase in Nontraumatic Hemorrhagic Shock in Rats.
30451726	6	147	theme	nonresuscitated	1060:1074	arg1	NTHS					1076:1079	nonresuscitated NTHS	1060:1079	nonresuscitated NTHS	1060:1079	Animals were fluid resuscitated with Ringer's acetate, balanced hydroxyethyl starch (HES) solution, or 0.9% normal saline to a mean arterial pressure of 80 mm Hg and compared with shams or nonresuscitated NTHS.
30451726	11	148	theme	interval	1650:1657	arg1	[CI					1659:1661	-1668; 95% confidence interval [CI	1628:1661	-1668; 95% confidence interval [CI	1628:1661	Syndecan-1 significantly increased in NTHS (mean difference, -1668; 95% confidence interval [CI], -2336 to -1001; P < .0001), balanced crystalloid (mean difference, -964.2; 95% CI, -1492 to -436.4; P = .0001), and HES (mean difference, -1030; 95% CI, -1594 to -465.8; P = .0001) groups at the end of the experiment compared to baseline.
30451726	11	149	dep	difference	1616:1625	arg1	[CI					1659:1661	-1668; 95% confidence interval [CI	1628:1661	-1668; 95% confidence interval [CI	1628:1661	Syndecan-1 significantly increased in NTHS (mean difference, -1668; 95% confidence interval [CI], -2336 to -1001; P < .0001), balanced crystalloid (mean difference, -964.2; 95% CI, -1492 to -436.4; P = .0001), and HES (mean difference, -1030; 95% CI, -1594 to -465.8; P = .0001) groups at the end of the experiment compared to baseline.
30451726	1	150	theme	tissue	329:334	arg1	edema					336:340	tissue edema	329:340	tissue edema	329:340	BACKGROUND Glycocalyx shedding after traumatic hemorrhagic or septic shock, as well as different resuscitation fluids, has been causally linked to increased vascular barrier permeability (VBP) resulting in tissue edema.
30451726	11	151	theme	%	1637:1637	arg1	[CI					1659:1661	-1668; 95% confidence interval [CI	1628:1661	-1668; 95% confidence interval [CI	1628:1661	Syndecan-1 significantly increased in NTHS (mean difference, -1668; 95% confidence interval [CI], -2336 to -1001; P < .0001), balanced crystalloid (mean difference, -964.2; 95% CI, -1492 to -436.4; P = .0001), and HES (mean difference, -1030; 95% CI, -1594 to -465.8; P = .0001) groups at the end of the experiment compared to baseline.
30451726	12	152	dep	-745.5	2080:2085	arg1	to					2077:2078	to	2077:2078	to	2077:2078	Hyaluronan levels were higher at the end of the experiment in nonresuscitated NTHS (-923.1; 95% CI, -1216 to -630; P = .0001) and balanced crystalloid (-1039; 95% CI, -1332 to -745.5; P = .0001) or HES (-394.2; 95% CI, -670.1 to -118.3; P = .0027) groups compared to controls.
30451726	14	153	theme	perfused	2526:2533	arg1	density					2542:2548	the perfused vessel density	2522:2548	the perfused vessel density	2522:2548	Total vessel density was altered in the normal saline (mean difference, 4.092; 95% CI, 0.6195-7.564; P = .016) and hemorrhagic shock (mean difference, 5.022; 95% CI, 1.55-8.495; P = .0024) groups compared to the control group, as well as the perfused vessel density and mean flow index.
30451726	9	154	theme	70-kDa	1354:1359	arg1	dyes					1320:1323	3 fluorescent dyes	1306:1323	3 fluorescent dyes (40- and 500-kDa dextran and 70-kDa albumin)	1306:1368	VBP changes were assessed using plasma decay of 3 fluorescent dyes (40- and 500-kDa dextran and 70-kDa albumin), Evans blue dye exclusion, intravital fluorescence microscopy, and determination of tissue edema (wet/dry weight ratio).
30451726	9	154	theme	70-kDa	1354:1359	arg1	albumin					1361:1367	70-kDa albumin	1354:1367	70-kDa albumin	1354:1367	VBP changes were assessed using plasma decay of 3 fluorescent dyes (40- and 500-kDa dextran and 70-kDa albumin), Evans blue dye exclusion, intravital fluorescence microscopy, and determination of tissue edema (wet/dry weight ratio).
31131431	0	0	theme	transplanted	79:90	arg1	islets					103:108	freely transplanted pancreatic islets	72:108	freely transplanted pancreatic islets	72:108	Nerve/glial antigen 2 is crucially involved in the revascularization of freely transplanted pancreatic islets.
31131431	5	1	theme	CD31	677:680	arg1	detection					628:636	immunohistochemical detection	608:636	immunohistochemical detection of insulin, glucagon, somatostatin and CD31	608:680	Islets were isolated from NG2+/+ (wild-type) and NG2-/- mice and their cellular composition was analyzed by immunohistochemical detection of insulin, glucagon, somatostatin and CD31.
31131431	9	2	theme	lower	1127:1131	arg1	density					1154:1160	a significantly lower functional capillary density	1111:1160	a significantly lower functional capillary density	1111:1160	However, transplanted NG2-/- islets exhibited a significantly lower functional capillary density and a reduced number of CD31-positive microvessels.
31131431	0	3	theme	freely	72:77	arg1	islets					103:108	freely transplanted pancreatic islets	72:108	freely transplanted pancreatic islets	72:108	Nerve/glial antigen 2 is crucially involved in the revascularization of freely transplanted pancreatic islets.
31131431	7	4	theme	mice	860:863	arg1	chambers					838:845	dorsal skinfold chambers	822:845	dorsal skinfold chambers of wild-type mice	822:863	In addition, isolated islets were transplanted into dorsal skinfold chambers of wild-type mice and their revascularization was determined by intravital fluorescence microscopy and immunohistochemistry.
31131431	7	5	theme	intravital	911:920	arg1	microscopy					935:944	intravital fluorescence microscopy	911:944	intravital fluorescence microscopy	911:944	In addition, isolated islets were transplanted into dorsal skinfold chambers of wild-type mice and their revascularization was determined by intravital fluorescence microscopy and immunohistochemistry.
31131431	6	6	link	enzyme-linked	727:739	arg1	ELISA					762:766	ELISA	762:766	ELISA	762:766	Moreover, insulin secretion was assessed by enzyme-linked immunosorbent assay (ELISA).
31131431	6	6	link	enzyme-linked	727:739	arg1	assay					755:759	enzyme-linked immunosorbent assay	727:759	enzyme-linked immunosorbent assay (ELISA)	727:767	Moreover, insulin secretion was assessed by enzyme-linked immunosorbent assay (ELISA).
31131431	0	7	theme	islets	103:108	arg1	revascularization					51:67	the revascularization	47:67	the revascularization of freely transplanted pancreatic islets	47:108	Nerve/glial antigen 2 is crucially involved in the revascularization of freely transplanted pancreatic islets.
31131431	4	8	theme	surface	428:434	arg1	protein					436:442	this surface protein	423:442	this surface protein	423:442	Therefore, we herein analyze whether this surface protein contributes to the revascularization of grafted islets.
31131431	7	9	theme	fluorescence	922:933	arg1	microscopy					935:944	intravital fluorescence microscopy	911:944	intravital fluorescence microscopy	911:944	In addition, isolated islets were transplanted into dorsal skinfold chambers of wild-type mice and their revascularization was determined by intravital fluorescence microscopy and immunohistochemistry.
31131431	10	10	theme	transplanted	1295:1306	arg1	islets					1308:1313	transplanted islets	1295:1313	transplanted islets	1295:1313	These findings demonstrate that the loss of NG2 impairs the revascularization of transplanted islets, underlining the importance of this pericytic proteoglycan for islet engraftment.
31131431	0	11	theme	pancreatic	92:101	arg1	islets					103:108	freely transplanted pancreatic islets	72:108	freely transplanted pancreatic islets	72:108	Nerve/glial antigen 2 is crucially involved in the revascularization of freely transplanted pancreatic islets.
31131431	5	12	theme	insulin	641:647	arg1	detection					628:636	immunohistochemical detection	608:636	immunohistochemical detection of insulin, glucagon, somatostatin and CD31	608:680	Islets were isolated from NG2+/+ (wild-type) and NG2-/- mice and their cellular composition was analyzed by immunohistochemical detection of insulin, glucagon, somatostatin and CD31.
31131431	9	13	theme	CD31-positive	1186:1198	arg1	microvessels					1200:1211	CD31-positive microvessels	1186:1211	CD31-positive microvessels	1186:1211	However, transplanted NG2-/- islets exhibited a significantly lower functional capillary density and a reduced number of CD31-positive microvessels.
31131431	9	14	theme	reduced	1168:1174	arg1	number					1176:1181	a reduced number	1166:1181	a reduced number of CD31-positive microvessels	1166:1211	However, transplanted NG2-/- islets exhibited a significantly lower functional capillary density and a reduced number of CD31-positive microvessels.
31131431	3	15	theme	proteoglycan	281:292	arg1	regulator					359:367	a crucial regulator	349:367	a crucial regulator of angiogenesis	349:383	The proteoglycan, nerve/glial antigen 2 (NG2) expressed in pericytes is a crucial regulator of angiogenesis.
31131431	3	15	theme	proteoglycan	281:292	arg1	NG2					318:320	The proteoglycan, nerve/glial antigen 2 (NG2) expressed in pericytes	277:344	NG2	318:320	The proteoglycan, nerve/glial antigen 2 (NG2) expressed in pericytes is a crucial regulator of angiogenesis.
31131431	8	16	theme	insulin	1046:1052	arg1	secretion					1054:1062	insulin secretion	1046:1062	insulin secretion	1046:1062	NG2+/+ and NG2-/- islets did not differ in their cellular composition and insulin secretion.
31131431	6	17	theme	immunosorbent	741:753	arg1	ELISA					762:766	ELISA	762:766	ELISA	762:766	Moreover, insulin secretion was assessed by enzyme-linked immunosorbent assay (ELISA).
31131431	6	17	theme	immunosorbent	741:753	arg1	assay					755:759	enzyme-linked immunosorbent assay	727:759	enzyme-linked immunosorbent assay (ELISA)	727:767	Moreover, insulin secretion was assessed by enzyme-linked immunosorbent assay (ELISA).
31131431	6	18	theme	enzyme-linked	727:739	arg1	ELISA					762:766	ELISA	762:766	ELISA	762:766	Moreover, insulin secretion was assessed by enzyme-linked immunosorbent assay (ELISA).
31131431	6	18	theme	enzyme-linked	727:739	arg1	assay					755:759	enzyme-linked immunosorbent assay	727:759	enzyme-linked immunosorbent assay (ELISA)	727:767	Moreover, insulin secretion was assessed by enzyme-linked immunosorbent assay (ELISA).
31131431	5	19	theme	somatostatin	660:671	arg1	detection					628:636	immunohistochemical detection	608:636	immunohistochemical detection of insulin, glucagon, somatostatin and CD31	608:680	Islets were isolated from NG2+/+ (wild-type) and NG2-/- mice and their cellular composition was analyzed by immunohistochemical detection of insulin, glucagon, somatostatin and CD31.
31131431	5	20	theme	wild-type	534:542	arg1	NG2+/+					526:531	NG2+/+ (wild-type) and NG2-/- mice	526:559	NG2+/+	526:531	Islets were isolated from NG2+/+ (wild-type) and NG2-/- mice and their cellular composition was analyzed by immunohistochemical detection of insulin, glucagon, somatostatin and CD31.
31131431	0	21	theme	Nerve/glial	0:10	arg1	antigen					12:18	Nerve/glial antigen	0:18	Nerve/glial antigen 2	0:20	Nerve/glial antigen 2 is crucially involved in the revascularization of freely transplanted pancreatic islets.
31131431	5	22	theme	glucagon	650:657	arg1	detection					628:636	immunohistochemical detection	608:636	immunohistochemical detection of insulin, glucagon, somatostatin and CD31	608:680	Islets were isolated from NG2+/+ (wild-type) and NG2-/- mice and their cellular composition was analyzed by immunohistochemical detection of insulin, glucagon, somatostatin and CD31.
31131431	9	23	theme	microvessels	1200:1211	arg1	density					1154:1160	a significantly lower functional capillary density	1111:1160	a significantly lower functional capillary density	1111:1160	However, transplanted NG2-/- islets exhibited a significantly lower functional capillary density and a reduced number of CD31-positive microvessels.
31131431	9	23	theme	microvessels	1200:1211	arg1	number					1176:1181	a reduced number	1166:1181	a reduced number of CD31-positive microvessels	1166:1211	However, transplanted NG2-/- islets exhibited a significantly lower functional capillary density and a reduced number of CD31-positive microvessels.
31131431	3	24	theme	angiogenesis	372:383	arg1	NG2					318:320	The proteoglycan, nerve/glial antigen 2 (NG2) expressed in pericytes	277:344	NG2	318:320	The proteoglycan, nerve/glial antigen 2 (NG2) expressed in pericytes is a crucial regulator of angiogenesis.
31131431	3	24	theme	angiogenesis	372:383	arg1	regulator					359:367	a crucial regulator	349:367	a crucial regulator of angiogenesis	349:383	The proteoglycan, nerve/glial antigen 2 (NG2) expressed in pericytes is a crucial regulator of angiogenesis.
31131431	5	25	theme	immunohistochemical	608:626	arg1	detection					628:636	immunohistochemical detection	608:636	immunohistochemical detection of insulin, glucagon, somatostatin and CD31	608:680	Islets were isolated from NG2+/+ (wild-type) and NG2-/- mice and their cellular composition was analyzed by immunohistochemical detection of insulin, glucagon, somatostatin and CD31.
31131431	10	26	theme	islets	1308:1313	arg1	revascularization					1274:1290	the revascularization	1270:1290	the revascularization of transplanted islets	1270:1313	These findings demonstrate that the loss of NG2 impairs the revascularization of transplanted islets, underlining the importance of this pericytic proteoglycan for islet engraftment.
31131431	7	27	theme	dorsal	822:827	arg1	chambers					838:845	dorsal skinfold chambers	822:845	dorsal skinfold chambers of wild-type mice	822:863	In addition, isolated islets were transplanted into dorsal skinfold chambers of wild-type mice and their revascularization was determined by intravital fluorescence microscopy and immunohistochemistry.
31131431	1	28	theme	Pancreatic	111:120	arg1	units					163:167	highly vascularized endocrine units	133:167	highly vascularized endocrine units	133:167	Pancreatic islets are highly vascularized endocrine units.
31131431	1	28	theme	Pancreatic	111:120	arg1	islets					122:127	Pancreatic islets	111:127	Pancreatic islets	111:127	Pancreatic islets are highly vascularized endocrine units.
31131431	7	29	theme	wild-type	850:858	arg1	mice					860:863	wild-type mice	850:863	wild-type mice	850:863	In addition, isolated islets were transplanted into dorsal skinfold chambers of wild-type mice and their revascularization was determined by intravital fluorescence microscopy and immunohistochemistry.
31131431	7	30	theme	skinfold	829:836	arg1	chambers					838:845	dorsal skinfold chambers	822:845	dorsal skinfold chambers of wild-type mice	822:863	In addition, isolated islets were transplanted into dorsal skinfold chambers of wild-type mice and their revascularization was determined by intravital fluorescence microscopy and immunohistochemistry.
31131431	2	31	theme	adequate	189:196	arg1	revascularization					198:214	their adequate revascularization	183:214	their adequate revascularization	183:214	Accordingly, their adequate revascularization is of major importance for successful islet transplantation.
31131431	9	32	theme	functional	1133:1142	arg1	density					1154:1160	a significantly lower functional capillary density	1111:1160	a significantly lower functional capillary density	1111:1160	However, transplanted NG2-/- islets exhibited a significantly lower functional capillary density and a reduced number of CD31-positive microvessels.
31131431	6	33	theme	insulin	693:699	arg1	secretion					701:709	insulin secretion	693:709	insulin secretion	693:709	Moreover, insulin secretion was assessed by enzyme-linked immunosorbent assay (ELISA).
31131431	3	34	theme	antigen	307:313	arg1	regulator					359:367	a crucial regulator	349:367	a crucial regulator of angiogenesis	349:383	The proteoglycan, nerve/glial antigen 2 (NG2) expressed in pericytes is a crucial regulator of angiogenesis.
31131431	3	34	theme	antigen	307:313	arg1	NG2					318:320	The proteoglycan, nerve/glial antigen 2 (NG2) expressed in pericytes	277:344	NG2	318:320	The proteoglycan, nerve/glial antigen 2 (NG2) expressed in pericytes is a crucial regulator of angiogenesis.
31131431	4	35	theme	islets	492:497	arg1	revascularization					463:479	the revascularization	459:479	the revascularization of grafted islets	459:497	Therefore, we herein analyze whether this surface protein contributes to the revascularization of grafted islets.
31131431	2	36	theme	islet	254:258	arg1	transplantation					260:274	successful islet transplantation	243:274	successful islet transplantation	243:274	Accordingly, their adequate revascularization is of major importance for successful islet transplantation.
31131431	3	37	dep	NG2	318:320	arg1	nerve/glial					295:305	The proteoglycan, nerve/glial antigen 2 (NG2) expressed in pericytes	277:344	nerve/glial	295:305	The proteoglycan, nerve/glial antigen 2 (NG2) expressed in pericytes is a crucial regulator of angiogenesis.
31131431	3	37	dep	NG2	318:320	arg1	expressed					323:331	expressed	323:331	expressed in pericytes	323:344	The proteoglycan, nerve/glial antigen 2 (NG2) expressed in pericytes is a crucial regulator of angiogenesis.
31131431	4	38	theme	grafted	484:490	arg1	islets					492:497	grafted islets	484:497	grafted islets	484:497	Therefore, we herein analyze whether this surface protein contributes to the revascularization of grafted islets.
31131431	2	39	theme	successful	243:252	arg1	transplantation					260:274	successful islet transplantation	243:274	successful islet transplantation	243:274	Accordingly, their adequate revascularization is of major importance for successful islet transplantation.
31131431	8	40	theme	NG2-/-	983:988	arg1	islets					990:995	NG2-/- islets	983:995	NG2-/- islets	983:995	NG2+/+ and NG2-/- islets did not differ in their cellular composition and insulin secretion.
31131431	9	41	theme	transplanted	1074:1085	arg1	islets					1094:1099	transplanted NG2-/- islets	1074:1099	transplanted NG2-/- islets	1074:1099	However, transplanted NG2-/- islets exhibited a significantly lower functional capillary density and a reduced number of CD31-positive microvessels.
31131431	5	42	theme	cellular	571:578	arg1	composition					580:590	their cellular composition	565:590	their cellular composition	565:590	Islets were isolated from NG2+/+ (wild-type) and NG2-/- mice and their cellular composition was analyzed by immunohistochemical detection of insulin, glucagon, somatostatin and CD31.
31131431	9	43	theme	NG2-/-	1087:1092	arg1	islets					1094:1099	transplanted NG2-/- islets	1074:1099	transplanted NG2-/- islets	1074:1099	However, transplanted NG2-/- islets exhibited a significantly lower functional capillary density and a reduced number of CD31-positive microvessels.
31131431	5	44	attach	isolated	512:519	arg1	NG2+/+					526:531	NG2+/+ (wild-type) and NG2-/- mice	526:559	NG2+/+	526:531	Islets were isolated from NG2+/+ (wild-type) and NG2-/- mice and their cellular composition was analyzed by immunohistochemical detection of insulin, glucagon, somatostatin and CD31.
31131431	5	44	attach	isolated	512:519	arg1	mice					556:559	NG2+/+ (wild-type) and NG2-/- mice	526:559	mice	556:559	Islets were isolated from NG2+/+ (wild-type) and NG2-/- mice and their cellular composition was analyzed by immunohistochemical detection of insulin, glucagon, somatostatin and CD31.
31131431	5	44	attach	isolated	512:519	arg2	Islets					500:505	Islets	500:505	Islets	500:505	Islets were isolated from NG2+/+ (wild-type) and NG2-/- mice and their cellular composition was analyzed by immunohistochemical detection of insulin, glucagon, somatostatin and CD31.
31131431	2	45	theme	major	222:226	arg1	importance					228:237	major importance	222:237	major importance for successful islet transplantation	222:274	Accordingly, their adequate revascularization is of major importance for successful islet transplantation.
31131431	8	46	theme	cellular	1021:1028	arg1	composition					1030:1040	their cellular composition	1015:1040	their cellular composition	1015:1040	NG2+/+ and NG2-/- islets did not differ in their cellular composition and insulin secretion.
31131431	10	47	theme	pericytic	1351:1359	arg1	proteoglycan					1361:1372	this pericytic proteoglycan	1346:1372	this pericytic proteoglycan	1346:1372	These findings demonstrate that the loss of NG2 impairs the revascularization of transplanted islets, underlining the importance of this pericytic proteoglycan for islet engraftment.
31131431	10	48	theme	islet	1378:1382	arg1	engraftment					1384:1394	islet engraftment	1378:1394	islet engraftment	1378:1394	These findings demonstrate that the loss of NG2 impairs the revascularization of transplanted islets, underlining the importance of this pericytic proteoglycan for islet engraftment.
31131431	1	49	theme	vascularized	140:151	arg1	units					163:167	highly vascularized endocrine units	133:167	highly vascularized endocrine units	133:167	Pancreatic islets are highly vascularized endocrine units.
31131431	1	49	theme	vascularized	140:151	arg1	islets					122:127	Pancreatic islets	111:127	Pancreatic islets	111:127	Pancreatic islets are highly vascularized endocrine units.
31131431	7	50	theme	isolated	783:790	arg1	islets					792:797	isolated islets	783:797	isolated islets	783:797	In addition, isolated islets were transplanted into dorsal skinfold chambers of wild-type mice and their revascularization was determined by intravital fluorescence microscopy and immunohistochemistry.
31131431	9	51	theme	capillary	1144:1152	arg1	density					1154:1160	a significantly lower functional capillary density	1111:1160	a significantly lower functional capillary density	1111:1160	However, transplanted NG2-/- islets exhibited a significantly lower functional capillary density and a reduced number of CD31-positive microvessels.
31131431	1	52	theme	endocrine	153:161	arg1	units					163:167	highly vascularized endocrine units	133:167	highly vascularized endocrine units	133:167	Pancreatic islets are highly vascularized endocrine units.
31131431	1	52	theme	endocrine	153:161	arg1	islets					122:127	Pancreatic islets	111:127	Pancreatic islets	111:127	Pancreatic islets are highly vascularized endocrine units.
31131431	3	53	theme	crucial	351:357	arg1	NG2					318:320	The proteoglycan, nerve/glial antigen 2 (NG2) expressed in pericytes	277:344	NG2	318:320	The proteoglycan, nerve/glial antigen 2 (NG2) expressed in pericytes is a crucial regulator of angiogenesis.
31131431	3	53	theme	crucial	351:357	arg1	regulator					359:367	a crucial regulator	349:367	a crucial regulator of angiogenesis	349:383	The proteoglycan, nerve/glial antigen 2 (NG2) expressed in pericytes is a crucial regulator of angiogenesis.
31131431	5	54	theme	NG2-/-	549:554	arg1	mice					556:559	NG2+/+ (wild-type) and NG2-/- mice	526:559	mice	556:559	Islets were isolated from NG2+/+ (wild-type) and NG2-/- mice and their cellular composition was analyzed by immunohistochemical detection of insulin, glucagon, somatostatin and CD31.
31131431	10	55	theme	proteoglycan	1361:1372	arg1	importance					1332:1341	the importance	1328:1341	the importance of this pericytic proteoglycan for islet engraftment	1328:1394	These findings demonstrate that the loss of NG2 impairs the revascularization of transplanted islets, underlining the importance of this pericytic proteoglycan for islet engraftment.
31131431	10	56	theme	NG2	1258:1260	arg1	loss					1250:1253	the loss	1246:1253	the loss of NG2	1246:1260	These findings demonstrate that the loss of NG2 impairs the revascularization of transplanted islets, underlining the importance of this pericytic proteoglycan for islet engraftment.
30424767	6	0	theme	Anti-inflammatory	815:831	arg1	model					833:837	Anti-inflammatory model	815:837	Anti-inflammatory model	815:837	Anti-inflammatory model was established in LPS-induced RAW264.7 cells.
30424767	11	1	dep	RESULTS	1315:1321	arg1	total					1325:1329	A total	1323:1329	RESULTS A total of eight compounds	1315:1348	RESULTS A total of eight compounds were identified, of which icariside II was the most abundant compound.
30424767	5	2	with	comparison	778:787	arg1	standards					804:812	reference standards	794:812	reference standards	794:812	METHODS In order to explore the composition of YYHs, YYHs was analyzed using high performance liquid chromatography-mass spectrometry-mass spectrometry (HPLC-MS/MS) and in comparison with reference standards.
30424767	15	3	theme	inflammatory	1822:1833	arg1	response					1835:1842	LPS-induced inflammatory response	1810:1842	LPS-induced inflammatory response	1810:1842	CONCLUSION In brief, YYHs contributed to the inhibition of LPS-induced inflammatory response through the TLR4/MD-2-mediated NF-κB pathway and may be a potential choice to combat inflammation diseases.
30424767	8	4	theme	necrosis	977:984	arg1	TNF-α					1000:1004	TNF-α	1000:1004	TNF-α	1000:1004	Production of tumor necrosis factor-alpha (TNF-α) and interleukin-2 (IL-2) were measured by enzyme-linked immunosorbent assays (ELISA).
30424767	8	4	theme	necrosis	977:984	arg1	factor-alpha					986:997	tumor necrosis factor-alpha	971:997	tumor necrosis factor-alpha (TNF-α)	971:1005	Production of tumor necrosis factor-alpha (TNF-α) and interleukin-2 (IL-2) were measured by enzyme-linked immunosorbent assays (ELISA).
30424767	2	5	theme	kidney	267:272	arg1	"					278:278	the kidney Yang"	263:278	the kidney Yang"	263:278	Maxim., Ying-Yang-Huo in Chinese has been used as a traditional Chinese medicine and is deemed to "reinforce the kidney Yang".
30424767	12	6	theme	dose-dependent	1561:1574	arg1	manner					1576:1581	a dose-dependent manner	1559:1581	a dose-dependent manner	1559:1581	YYHs (12.5-50 μg/mL) had no obvious cytotoxic effect on cells, and remarkably inhibited LPS-induced production of NO, TNF-α and IL-2 with a dose-dependent manner.
30424767	11	7	theme	compounds	1340:1348	arg1	total					1325:1329	A total	1323:1329	RESULTS A total of eight compounds	1315:1348	RESULTS A total of eight compounds were identified, of which icariside II was the most abundant compound.
30424767	5	8	theme	high	683:686	arg1	HPLC-MS/MS					759:768	HPLC-MS/MS	759:768	HPLC-MS/MS	759:768	METHODS In order to explore the composition of YYHs, YYHs was analyzed using high performance liquid chromatography-mass spectrometry-mass spectrometry (HPLC-MS/MS) and in comparison with reference standards.
30424767	5	8	theme	high	683:686	arg1	spectrometry					745:756	high performance liquid chromatography-mass spectrometry-mass spectrometry	683:756	high performance liquid chromatography-mass spectrometry-mass spectrometry (HPLC-MS/MS)	683:769	METHODS In order to explore the composition of YYHs, YYHs was analyzed using high performance liquid chromatography-mass spectrometry-mass spectrometry (HPLC-MS/MS) and in comparison with reference standards.
30424767	3	9	theme	Previous	281:288	arg1	studies					290:296	Previous studies	281:296	Previous studies	281:296	Previous studies showed that E. sagittatum could modulate the immune system and treat some chronic disease such as rheumatic arthritis, cardiovascular diseases and osteoporosis.
30424767	9	10	located	detected	1160:1167	arg2	expression					1106:1115	expression	1106:1115	expression of p-p65 protein and TLR4/MD-2 complex	1106:1154	In addition, expression of p-p65 protein and TLR4/MD-2 complex was detected by western blots and flow cytometric, respectively.
30424767	9	10	located	detected	1160:1167	arg1	addition					1096:1103	addition	1096:1103	addition	1096:1103	In addition, expression of p-p65 protein and TLR4/MD-2 complex was detected by western blots and flow cytometric, respectively.
30424767	5	11	theme	YYHs	659:662	arg1	composition					638:648	the composition	634:648	the composition of YYHs, YYHs	634:662	METHODS In order to explore the composition of YYHs, YYHs was analyzed using high performance liquid chromatography-mass spectrometry-mass spectrometry (HPLC-MS/MS) and in comparison with reference standards.
30424767	15	12	dep	CONCLUSION	1751:1760	arg1	brief					1765:1769	brief	1765:1769	brief	1765:1769	CONCLUSION In brief, YYHs contributed to the inhibition of LPS-induced inflammatory response through the TLR4/MD-2-mediated NF-κB pathway and may be a potential choice to combat inflammation diseases.
30424767	12	13	theme	LPS-induced	1509:1519	arg1	production					1521:1530	LPS-induced production	1509:1530	LPS-induced production of NO, TNF-α and IL-2	1509:1552	YYHs (12.5-50 μg/mL) had no obvious cytotoxic effect on cells, and remarkably inhibited LPS-induced production of NO, TNF-α and IL-2 with a dose-dependent manner.
30424767	12	14	contain	had	1442:1444	arg1	YYHs					1421:1424	YYHs	1421:1424	YYHs (12.5-50 μg/mL)	1421:1440	YYHs (12.5-50 μg/mL) had no obvious cytotoxic effect on cells, and remarkably inhibited LPS-induced production of NO, TNF-α and IL-2 with a dose-dependent manner.
30424767	12	14	contain	had	1442:1444	arg2	effect					1467:1472	no obvious cytotoxic effect	1446:1472	no obvious cytotoxic effect	1446:1472	YYHs (12.5-50 μg/mL) had no obvious cytotoxic effect on cells, and remarkably inhibited LPS-induced production of NO, TNF-α and IL-2 with a dose-dependent manner.
30424767	12	14	contain	had	1442:1444	arg1	12.5-50 μg/mL					1427:1439	12.5-50 μg/mL	1427:1439	12.5-50 μg/mL	1427:1439	YYHs (12.5-50 μg/mL) had no obvious cytotoxic effect on cells, and remarkably inhibited LPS-induced production of NO, TNF-α and IL-2 with a dose-dependent manner.
30424767	10	15	theme	B	1242:1242	arg1	translocation					1260:1272	Nuclear factor kappa B (NF-κB) nuclear translocation	1221:1272	Nuclear factor kappa B (NF-κB) nuclear translocation	1221:1272	Nuclear factor kappa B (NF-κB) nuclear translocation was observed by fluorescence microscope.
30424767	8	16	theme	interleukin-2	1011:1023	arg1	Production					957:966	Production	957:966	Production of tumor necrosis factor-alpha (TNF-α) and interleukin-2 (IL-2)	957:1030	Production of tumor necrosis factor-alpha (TNF-α) and interleukin-2 (IL-2) were measured by enzyme-linked immunosorbent assays (ELISA).
30424767	10	17	theme	factor	1229:1234	arg1	NF-κB					1245:1249	NF-κB	1245:1249	NF-κB	1245:1249	Nuclear factor kappa B (NF-κB) nuclear translocation was observed by fluorescence microscope.
30424767	10	17	theme	factor	1229:1234	arg1	B					1242:1242	Nuclear factor kappa B	1221:1242	Nuclear factor kappa B (NF-κB) nuclear translocation	1221:1272	Nuclear factor kappa B (NF-κB) nuclear translocation was observed by fluorescence microscope.
30424767	5	18	theme	liquid	700:705	arg1	HPLC-MS/MS					759:768	HPLC-MS/MS	759:768	HPLC-MS/MS	759:768	METHODS In order to explore the composition of YYHs, YYHs was analyzed using high performance liquid chromatography-mass spectrometry-mass spectrometry (HPLC-MS/MS) and in comparison with reference standards.
30424767	5	18	theme	liquid	700:705	arg1	spectrometry					745:756	high performance liquid chromatography-mass spectrometry-mass spectrometry	683:756	high performance liquid chromatography-mass spectrometry-mass spectrometry (HPLC-MS/MS)	683:769	METHODS In order to explore the composition of YYHs, YYHs was analyzed using high performance liquid chromatography-mass spectrometry-mass spectrometry (HPLC-MS/MS) and in comparison with reference standards.
30424767	7	19	theme	Griess	941:946	arg1	reagent					948:954	the Griess reagent	937:954	the Griess reagent	937:954	The levels of nitric oxide (NO) were measured with the Griess reagent.
30424767	15	20	theme	inflammation	1929:1940	arg1	diseases					1942:1949	inflammation diseases	1929:1949	inflammation diseases	1929:1949	CONCLUSION In brief, YYHs contributed to the inhibition of LPS-induced inflammatory response through the TLR4/MD-2-mediated NF-κB pathway and may be a potential choice to combat inflammation diseases.
30424767	10	21	theme	nuclear	1252:1258	arg1	translocation					1260:1272	Nuclear factor kappa B (NF-κB) nuclear translocation	1221:1272	Nuclear factor kappa B (NF-κB) nuclear translocation	1221:1272	Nuclear factor kappa B (NF-κB) nuclear translocation was observed by fluorescence microscope.
30424767	5	22	theme	spectrometry-mass	727:743	arg1	HPLC-MS/MS					759:768	HPLC-MS/MS	759:768	HPLC-MS/MS	759:768	METHODS In order to explore the composition of YYHs, YYHs was analyzed using high performance liquid chromatography-mass spectrometry-mass spectrometry (HPLC-MS/MS) and in comparison with reference standards.
30424767	5	22	theme	spectrometry-mass	727:743	arg1	spectrometry					745:756	high performance liquid chromatography-mass spectrometry-mass spectrometry	683:756	high performance liquid chromatography-mass spectrometry-mass spectrometry (HPLC-MS/MS)	683:769	METHODS In order to explore the composition of YYHs, YYHs was analyzed using high performance liquid chromatography-mass spectrometry-mass spectrometry (HPLC-MS/MS) and in comparison with reference standards.
30424767	16	23	theme	pathways	1976:1983	arg1	schema					1966:1971	a schema	1964:1971	a schema of pathways	1964:1983	It includes a schema of pathways at the end of the paper.
30424767	3	24	theme	chronic	372:378	arg1	disease					380:386	some chronic disease	367:386	some chronic disease such as rheumatic arthritis, cardiovascular diseases and osteoporosis	367:456	Previous studies showed that E. sagittatum could modulate the immune system and treat some chronic disease such as rheumatic arthritis, cardiovascular diseases and osteoporosis.
30424767	4	25	theme	study	475:479	arg1	aim					463:465	The aim	459:465	The aim of this study	459:479	The aim of this study is to evaluate the anti-inflammatory effects of ethyl acetate extracts (YYHs) of E. sagittatum and its mechanisms of action.
30424767	13	26	theme	complex	1650:1656	arg1	expression					1616:1625	expression	1616:1625	expression of p-p65 and TLR4/MD-2 complex	1616:1656	Additionally, YYHs up-regulated expression of p-p65 and TLR4/MD-2 complex.
30424767	0	27	theme	Epimedium	0:8	arg1	sagittatum					10:19	Epimedium sagittatum	0:19	Epimedium sagittatum	0:19	Epimedium sagittatum inhibits TLR4/MD-2 mediated NF-κB signaling pathway with anti-inflammatory activity.
30424767	1	28	theme	Epimedium	117:125	arg1	sagittatum					127:136	BACKGROUND Epimedium sagittatum	106:136	BACKGROUND Epimedium sagittatum (Sieb.et Zucc.)	106:152	BACKGROUND Epimedium sagittatum (Sieb.et Zucc.)
30424767	1	28	theme	Epimedium	117:125	arg1	Zucc					147:150	Zucc	147:150	Zucc	147:150	BACKGROUND Epimedium sagittatum (Sieb.et Zucc.)
30424767	4	29	theme	extracts	543:550	arg1	mechanisms					584:593	its mechanisms	580:593	its mechanisms of action	580:603	The aim of this study is to evaluate the anti-inflammatory effects of ethyl acetate extracts (YYHs) of E. sagittatum and its mechanisms of action.
30424767	4	29	theme	extracts	543:550	arg1	effects					518:524	the anti-inflammatory effects	496:524	the anti-inflammatory effects of ethyl acetate extracts (YYHs) of E. sagittatum	496:574	The aim of this study is to evaluate the anti-inflammatory effects of ethyl acetate extracts (YYHs) of E. sagittatum and its mechanisms of action.
30424767	3	30	theme	rheumatic	396:404	arg1	arthritis					406:414	rheumatic arthritis	396:414	rheumatic arthritis	396:414	Previous studies showed that E. sagittatum could modulate the immune system and treat some chronic disease such as rheumatic arthritis, cardiovascular diseases and osteoporosis.
30424767	0	31	theme	mediated	40:47	arg1	pathway					65:71	TLR4/MD-2 mediated NF-κB signaling pathway	30:71	TLR4/MD-2 mediated NF-κB signaling pathway with anti-inflammatory activity	30:103	Epimedium sagittatum inhibits TLR4/MD-2 mediated NF-κB signaling pathway with anti-inflammatory activity.
30424767	6	32	theme	RAW264.7	870:877	arg1	cells					879:883	LPS-induced RAW264.7 cells	858:883	LPS-induced RAW264.7 cells	858:883	Anti-inflammatory model was established in LPS-induced RAW264.7 cells.
30424767	0	33	theme	signaling	55:63	arg1	pathway					65:71	TLR4/MD-2 mediated NF-κB signaling pathway	30:71	TLR4/MD-2 mediated NF-κB signaling pathway with anti-inflammatory activity	30:103	Epimedium sagittatum inhibits TLR4/MD-2 mediated NF-κB signaling pathway with anti-inflammatory activity.
30424767	4	34	theme	ethyl	529:533	arg1	acetate					535:541	ethyl acetate	529:541	ethyl acetate extracts (YYHs) of E. sagittatum	529:574	The aim of this study is to evaluate the anti-inflammatory effects of ethyl acetate extracts (YYHs) of E. sagittatum and its mechanisms of action.
30424767	2	35	theme	traditional	206:216	arg1	medicine					226:233	a traditional Chinese medicine	204:233	a traditional Chinese medicine	204:233	Maxim., Ying-Yang-Huo in Chinese has been used as a traditional Chinese medicine and is deemed to "reinforce the kidney Yang".
30424767	2	35	theme	traditional	206:216	arg1	Ying-Yang-Huo					162:174	Ying-Yang-Huo	162:174	Ying-Yang-Huo	162:174	Maxim., Ying-Yang-Huo in Chinese has been used as a traditional Chinese medicine and is deemed to "reinforce the kidney Yang".
30424767	9	36	theme	p-p65	1120:1124	arg1	protein					1126:1132	p-p65 protein	1120:1132	p-p65 protein	1120:1132	In addition, expression of p-p65 protein and TLR4/MD-2 complex was detected by western blots and flow cytometric, respectively.
30424767	12	37	theme	obvious	1449:1455	arg1	effect					1467:1472	no obvious cytotoxic effect	1446:1472	no obvious cytotoxic effect	1446:1472	YYHs (12.5-50 μg/mL) had no obvious cytotoxic effect on cells, and remarkably inhibited LPS-induced production of NO, TNF-α and IL-2 with a dose-dependent manner.
30424767	8	38	dep	enzyme-linked	1049:1061	arg1	immunosorbent					1063:1075	immunosorbent	1063:1075	immunosorbent	1063:1075	Production of tumor necrosis factor-alpha (TNF-α) and interleukin-2 (IL-2) were measured by enzyme-linked immunosorbent assays (ELISA).
30424767	14	39	theme	nuclear	1728:1734	arg1	translocation					1736:1748	NF-κB p65 nuclear translocation	1718:1748	NF-κB p65 nuclear translocation	1718:1748	Further research showed that YYHs significantly suppressed NF-κB p65 nuclear translocation.
30424767	15	40	theme	response	1835:1842	arg1	inhibition					1796:1805	the inhibition	1792:1805	the inhibition of LPS-induced inflammatory response through the TLR4/MD-2-mediated NF-κB pathway	1792:1887	CONCLUSION In brief, YYHs contributed to the inhibition of LPS-induced inflammatory response through the TLR4/MD-2-mediated NF-κB pathway and may be a potential choice to combat inflammation diseases.
30424767	9	41	theme	complex	1148:1154	arg1	expression					1106:1115	expression	1106:1115	expression of p-p65 protein and TLR4/MD-2 complex	1106:1154	In addition, expression of p-p65 protein and TLR4/MD-2 complex was detected by western blots and flow cytometric, respectively.
30424767	7	42	theme	nitric	900:905	arg1	NO					914:915	NO	914:915	NO	914:915	The levels of nitric oxide (NO) were measured with the Griess reagent.
30424767	7	42	theme	nitric	900:905	arg1	oxide					907:911	nitric oxide	900:911	nitric oxide (NO)	900:916	The levels of nitric oxide (NO) were measured with the Griess reagent.
30424767	14	43	theme	NF-κB	1718:1722	arg1	translocation					1736:1748	NF-κB p65 nuclear translocation	1718:1748	NF-κB p65 nuclear translocation	1718:1748	Further research showed that YYHs significantly suppressed NF-κB p65 nuclear translocation.
30424767	8	44	theme	tumor	971:975	arg1	TNF-α					1000:1004	TNF-α	1000:1004	TNF-α	1000:1004	Production of tumor necrosis factor-alpha (TNF-α) and interleukin-2 (IL-2) were measured by enzyme-linked immunosorbent assays (ELISA).
30424767	8	44	theme	tumor	971:975	arg1	factor-alpha					986:997	tumor necrosis factor-alpha	971:997	tumor necrosis factor-alpha (TNF-α)	971:1005	Production of tumor necrosis factor-alpha (TNF-α) and interleukin-2 (IL-2) were measured by enzyme-linked immunosorbent assays (ELISA).
30424767	15	45	theme	LPS-induced	1810:1820	arg1	response					1835:1842	LPS-induced inflammatory response	1810:1842	LPS-induced inflammatory response	1810:1842	CONCLUSION In brief, YYHs contributed to the inhibition of LPS-induced inflammatory response through the TLR4/MD-2-mediated NF-κB pathway and may be a potential choice to combat inflammation diseases.
30424767	8	46	theme	factor-alpha	986:997	arg1	Production					957:966	Production	957:966	Production of tumor necrosis factor-alpha (TNF-α) and interleukin-2 (IL-2)	957:1030	Production of tumor necrosis factor-alpha (TNF-α) and interleukin-2 (IL-2) were measured by enzyme-linked immunosorbent assays (ELISA).
30424767	4	47	theme	sagittatum	565:574	arg1	YYHs					553:556	YYHs	553:556	YYHs	553:556	The aim of this study is to evaluate the anti-inflammatory effects of ethyl acetate extracts (YYHs) of E. sagittatum and its mechanisms of action.
30424767	4	47	theme	sagittatum	565:574	arg1	extracts					543:550	ethyl acetate extracts	529:550	ethyl acetate extracts (YYHs) of E. sagittatum	529:574	The aim of this study is to evaluate the anti-inflammatory effects of ethyl acetate extracts (YYHs) of E. sagittatum and its mechanisms of action.
30424767	11	48	theme	which	1370:1374	arg1	icariside					1376:1384	icariside II	1376:1387	icariside II	1376:1387	RESULTS A total of eight compounds were identified, of which icariside II was the most abundant compound.
30424767	11	48	theme	which	1370:1374	arg1	compound					1411:1418	the most abundant compound	1393:1418	the most abundant compound	1393:1418	RESULTS A total of eight compounds were identified, of which icariside II was the most abundant compound.
30424767	9	49	theme	western	1172:1178	arg1	blots					1180:1184	western blots	1172:1184	western blots and flow cytometric	1172:1204	In addition, expression of p-p65 protein and TLR4/MD-2 complex was detected by western blots and flow cytometric, respectively.
30424767	2	50	theme	Yang	274:277	arg1	"					278:278	the kidney Yang"	263:278	the kidney Yang"	263:278	Maxim., Ying-Yang-Huo in Chinese has been used as a traditional Chinese medicine and is deemed to "reinforce the kidney Yang".
30424767	15	51	theme	potential	1902:1910	arg1	choice					1912:1917	a potential choice	1900:1917	a potential choice to combat inflammation diseases	1900:1949	CONCLUSION In brief, YYHs contributed to the inhibition of LPS-induced inflammatory response through the TLR4/MD-2-mediated NF-κB pathway and may be a potential choice to combat inflammation diseases.
30424767	9	52	theme	cytometric	1195:1204	arg1	blots					1180:1184	western blots	1172:1184	western blots and flow cytometric	1172:1204	In addition, expression of p-p65 protein and TLR4/MD-2 complex was detected by western blots and flow cytometric, respectively.
30424767	12	53	theme	TNF-α	1539:1543	arg1	production					1521:1530	LPS-induced production	1509:1530	LPS-induced production of NO, TNF-α and IL-2	1509:1552	YYHs (12.5-50 μg/mL) had no obvious cytotoxic effect on cells, and remarkably inhibited LPS-induced production of NO, TNF-α and IL-2 with a dose-dependent manner.
30424767	15	54	theme	NF-κB	1875:1879	arg1	pathway					1881:1887	the TLR4/MD-2-mediated NF-κB pathway	1852:1887	the TLR4/MD-2-mediated NF-κB pathway	1852:1887	CONCLUSION In brief, YYHs contributed to the inhibition of LPS-induced inflammatory response through the TLR4/MD-2-mediated NF-κB pathway and may be a potential choice to combat inflammation diseases.
30424767	14	55	theme	Further	1659:1665	arg1	research					1667:1674	Further research	1659:1674	Further research	1659:1674	Further research showed that YYHs significantly suppressed NF-κB p65 nuclear translocation.
30424767	12	56	theme	IL-2	1549:1552	arg1	production					1521:1530	LPS-induced production	1509:1530	LPS-induced production of NO, TNF-α and IL-2	1509:1552	YYHs (12.5-50 μg/mL) had no obvious cytotoxic effect on cells, and remarkably inhibited LPS-induced production of NO, TNF-α and IL-2 with a dose-dependent manner.
30424767	10	57	theme	Nuclear	1221:1227	arg1	NF-κB					1245:1249	NF-κB	1245:1249	NF-κB	1245:1249	Nuclear factor kappa B (NF-κB) nuclear translocation was observed by fluorescence microscope.
30424767	10	57	theme	Nuclear	1221:1227	arg1	B					1242:1242	Nuclear factor kappa B	1221:1242	Nuclear factor kappa B (NF-κB) nuclear translocation	1221:1272	Nuclear factor kappa B (NF-κB) nuclear translocation was observed by fluorescence microscope.
30424767	0	58	theme	anti-inflammatory	78:94	arg1	activity					96:103	anti-inflammatory activity	78:103	anti-inflammatory activity	78:103	Epimedium sagittatum inhibits TLR4/MD-2 mediated NF-κB signaling pathway with anti-inflammatory activity.
30424767	12	59	theme	NO	1535:1536	arg1	production					1521:1530	LPS-induced production	1509:1530	LPS-induced production of NO, TNF-α and IL-2	1509:1552	YYHs (12.5-50 μg/mL) had no obvious cytotoxic effect on cells, and remarkably inhibited LPS-induced production of NO, TNF-α and IL-2 with a dose-dependent manner.
30424767	9	60	dep	cytometric	1195:1204	arg1	flow					1190:1193	flow	1190:1193	flow	1190:1193	In addition, expression of p-p65 protein and TLR4/MD-2 complex was detected by western blots and flow cytometric, respectively.
30424767	5	61	dep	analyzed	668:675	arg1	comparison					778:787	comparison	778:787	comparison with reference standards	778:812	METHODS In order to explore the composition of YYHs, YYHs was analyzed using high performance liquid chromatography-mass spectrometry-mass spectrometry (HPLC-MS/MS) and in comparison with reference standards.
30424767	5	61	dep	analyzed	668:675	arg1	using					677:681	using	677:681	using high performance liquid chromatography-mass spectrometry-mass spectrometry (HPLC-MS/MS)	677:769	METHODS In order to explore the composition of YYHs, YYHs was analyzed using high performance liquid chromatography-mass spectrometry-mass spectrometry (HPLC-MS/MS) and in comparison with reference standards.
30424767	4	62	theme	anti-inflammatory	500:516	arg1	effects					518:524	the anti-inflammatory effects	496:524	the anti-inflammatory effects of ethyl acetate extracts (YYHs) of E. sagittatum	496:574	The aim of this study is to evaluate the anti-inflammatory effects of ethyl acetate extracts (YYHs) of E. sagittatum and its mechanisms of action.
30424767	10	63	theme	kappa	1236:1240	arg1	NF-κB					1245:1249	NF-κB	1245:1249	NF-κB	1245:1249	Nuclear factor kappa B (NF-κB) nuclear translocation was observed by fluorescence microscope.
30424767	10	63	theme	kappa	1236:1240	arg1	B					1242:1242	Nuclear factor kappa B	1221:1242	Nuclear factor kappa B (NF-κB) nuclear translocation	1221:1272	Nuclear factor kappa B (NF-κB) nuclear translocation was observed by fluorescence microscope.
30424767	5	64	theme	performance	688:698	arg1	HPLC-MS/MS					759:768	HPLC-MS/MS	759:768	HPLC-MS/MS	759:768	METHODS In order to explore the composition of YYHs, YYHs was analyzed using high performance liquid chromatography-mass spectrometry-mass spectrometry (HPLC-MS/MS) and in comparison with reference standards.
30424767	5	64	theme	performance	688:698	arg1	spectrometry					745:756	high performance liquid chromatography-mass spectrometry-mass spectrometry	683:756	high performance liquid chromatography-mass spectrometry-mass spectrometry (HPLC-MS/MS)	683:769	METHODS In order to explore the composition of YYHs, YYHs was analyzed using high performance liquid chromatography-mass spectrometry-mass spectrometry (HPLC-MS/MS) and in comparison with reference standards.
30424767	0	65	with	pathway	65:71	arg1	activity					96:103	anti-inflammatory activity	78:103	anti-inflammatory activity	78:103	Epimedium sagittatum inhibits TLR4/MD-2 mediated NF-κB signaling pathway with anti-inflammatory activity.
30424767	11	66	theme	abundant	1402:1409	arg1	icariside					1376:1384	icariside II	1376:1387	icariside II	1376:1387	RESULTS A total of eight compounds were identified, of which icariside II was the most abundant compound.
30424767	11	66	theme	abundant	1402:1409	arg1	compound					1411:1418	the most abundant compound	1393:1418	the most abundant compound	1393:1418	RESULTS A total of eight compounds were identified, of which icariside II was the most abundant compound.
30424767	5	67	theme	chromatography-mass	707:725	arg1	HPLC-MS/MS					759:768	HPLC-MS/MS	759:768	HPLC-MS/MS	759:768	METHODS In order to explore the composition of YYHs, YYHs was analyzed using high performance liquid chromatography-mass spectrometry-mass spectrometry (HPLC-MS/MS) and in comparison with reference standards.
30424767	5	67	theme	chromatography-mass	707:725	arg1	spectrometry					745:756	high performance liquid chromatography-mass spectrometry-mass spectrometry	683:756	high performance liquid chromatography-mass spectrometry-mass spectrometry (HPLC-MS/MS)	683:769	METHODS In order to explore the composition of YYHs, YYHs was analyzed using high performance liquid chromatography-mass spectrometry-mass spectrometry (HPLC-MS/MS) and in comparison with reference standards.
30424767	2	68	used	used	196:199	arg2	medicine					226:233	a traditional Chinese medicine	204:233	a traditional Chinese medicine	204:233	Maxim., Ying-Yang-Huo in Chinese has been used as a traditional Chinese medicine and is deemed to "reinforce the kidney Yang".
30424767	2	68	used	used	196:199	arg2	Ying-Yang-Huo					162:174	Ying-Yang-Huo	162:174	Ying-Yang-Huo	162:174	Maxim., Ying-Yang-Huo in Chinese has been used as a traditional Chinese medicine and is deemed to "reinforce the kidney Yang".
30424767	13	69	theme	TLR4/MD-2	1640:1648	arg1	complex					1650:1656	TLR4/MD-2 complex	1640:1656	TLR4/MD-2 complex	1640:1656	Additionally, YYHs up-regulated expression of p-p65 and TLR4/MD-2 complex.
30424767	5	70	theme	reference	794:802	arg1	standards					804:812	reference standards	794:812	reference standards	794:812	METHODS In order to explore the composition of YYHs, YYHs was analyzed using high performance liquid chromatography-mass spectrometry-mass spectrometry (HPLC-MS/MS) and in comparison with reference standards.
30424767	8	71	theme	enzyme-linked	1049:1061	arg1	ELISA					1085:1089	ELISA	1085:1089	ELISA	1085:1089	Production of tumor necrosis factor-alpha (TNF-α) and interleukin-2 (IL-2) were measured by enzyme-linked immunosorbent assays (ELISA).
30424767	8	71	theme	enzyme-linked	1049:1061	arg1	assays					1077:1082	enzyme-linked immunosorbent assays	1049:1082	enzyme-linked immunosorbent assays (ELISA)	1049:1090	Production of tumor necrosis factor-alpha (TNF-α) and interleukin-2 (IL-2) were measured by enzyme-linked immunosorbent assays (ELISA).
30424767	1	72	theme	BACKGROUND	106:115	arg1	sagittatum					127:136	BACKGROUND Epimedium sagittatum	106:136	BACKGROUND Epimedium sagittatum (Sieb.et Zucc.)	106:152	BACKGROUND Epimedium sagittatum (Sieb.et Zucc.)
30424767	1	72	theme	BACKGROUND	106:115	arg1	Zucc					147:150	Zucc	147:150	Zucc	147:150	BACKGROUND Epimedium sagittatum (Sieb.et Zucc.)
30424767	10	73	theme	fluorescence	1290:1301	arg1	microscope					1303:1312	fluorescence microscope	1290:1312	fluorescence microscope	1290:1312	Nuclear factor kappa B (NF-κB) nuclear translocation was observed by fluorescence microscope.
30424767	3	74	theme	cardiovascular	417:430	arg1	diseases					432:439	cardiovascular diseases	417:439	cardiovascular diseases	417:439	Previous studies showed that E. sagittatum could modulate the immune system and treat some chronic disease such as rheumatic arthritis, cardiovascular diseases and osteoporosis.
30424767	0	75	theme	TLR4/MD-2	30:38	arg1	pathway					65:71	TLR4/MD-2 mediated NF-κB signaling pathway	30:71	TLR4/MD-2 mediated NF-κB signaling pathway with anti-inflammatory activity	30:103	Epimedium sagittatum inhibits TLR4/MD-2 mediated NF-κB signaling pathway with anti-inflammatory activity.
30424767	5	76	dep	explore	626:632	arg1	to					623:624	to	623:624	to	623:624	METHODS In order to explore the composition of YYHs, YYHs was analyzed using high performance liquid chromatography-mass spectrometry-mass spectrometry (HPLC-MS/MS) and in comparison with reference standards.
30424767	16	77	theme	paper	2003:2007	arg1	end					1992:1994	the end	1988:1994	the end of the paper	1988:2007	It includes a schema of pathways at the end of the paper.
30424767	0	78	theme	NF-κB	49:53	arg1	pathway					65:71	TLR4/MD-2 mediated NF-κB signaling pathway	30:71	TLR4/MD-2 mediated NF-κB signaling pathway with anti-inflammatory activity	30:103	Epimedium sagittatum inhibits TLR4/MD-2 mediated NF-κB signaling pathway with anti-inflammatory activity.
30424767	12	79	theme	cytotoxic	1457:1465	arg1	effect					1467:1472	no obvious cytotoxic effect	1446:1472	no obvious cytotoxic effect	1446:1472	YYHs (12.5-50 μg/mL) had no obvious cytotoxic effect on cells, and remarkably inhibited LPS-induced production of NO, TNF-α and IL-2 with a dose-dependent manner.
30424767	13	80	theme	p-p65	1630:1634	arg1	expression					1616:1625	expression	1616:1625	expression of p-p65 and TLR4/MD-2 complex	1616:1656	Additionally, YYHs up-regulated expression of p-p65 and TLR4/MD-2 complex.
30424767	3	81	theme	immune	343:348	arg1	system					350:355	the immune system	339:355	the immune system	339:355	Previous studies showed that E. sagittatum could modulate the immune system and treat some chronic disease such as rheumatic arthritis, cardiovascular diseases and osteoporosis.
30424767	6	82	theme	LPS-induced	858:868	arg1	cells					879:883	LPS-induced RAW264.7 cells	858:883	LPS-induced RAW264.7 cells	858:883	Anti-inflammatory model was established in LPS-induced RAW264.7 cells.
30424767	5	83	dep	METHODS	606:612	arg1	explore					626:632	explore	626:632	explore the composition of YYHs, YYHs	626:662	METHODS In order to explore the composition of YYHs, YYHs was analyzed using high performance liquid chromatography-mass spectrometry-mass spectrometry (HPLC-MS/MS) and in comparison with reference standards.
30424767	4	84	theme	acetate	535:541	arg1	YYHs					553:556	YYHs	553:556	YYHs	553:556	The aim of this study is to evaluate the anti-inflammatory effects of ethyl acetate extracts (YYHs) of E. sagittatum and its mechanisms of action.
30424767	4	84	theme	acetate	535:541	arg1	extracts					543:550	ethyl acetate extracts	529:550	ethyl acetate extracts (YYHs) of E. sagittatum	529:574	The aim of this study is to evaluate the anti-inflammatory effects of ethyl acetate extracts (YYHs) of E. sagittatum and its mechanisms of action.
30424767	2	85	theme	Chinese	218:224	arg1	medicine					226:233	a traditional Chinese medicine	204:233	a traditional Chinese medicine	204:233	Maxim., Ying-Yang-Huo in Chinese has been used as a traditional Chinese medicine and is deemed to "reinforce the kidney Yang".
30424767	2	85	theme	Chinese	218:224	arg1	Ying-Yang-Huo					162:174	Ying-Yang-Huo	162:174	Ying-Yang-Huo	162:174	Maxim., Ying-Yang-Huo in Chinese has been used as a traditional Chinese medicine and is deemed to "reinforce the kidney Yang".
30424767	9	86	theme	protein	1126:1132	arg1	expression					1106:1115	expression	1106:1115	expression of p-p65 protein and TLR4/MD-2 complex	1106:1154	In addition, expression of p-p65 protein and TLR4/MD-2 complex was detected by western blots and flow cytometric, respectively.
30424767	7	87	theme	oxide	907:911	arg1	levels					890:895	The levels	886:895	The levels of nitric oxide (NO)	886:916	The levels of nitric oxide (NO) were measured with the Griess reagent.
30424767	4	88	theme	action	598:603	arg1	mechanisms					584:593	its mechanisms	580:593	its mechanisms of action	580:603	The aim of this study is to evaluate the anti-inflammatory effects of ethyl acetate extracts (YYHs) of E. sagittatum and its mechanisms of action.
30424767	4	88	theme	action	598:603	arg1	effects					518:524	the anti-inflammatory effects	496:524	the anti-inflammatory effects of ethyl acetate extracts (YYHs) of E. sagittatum	496:574	The aim of this study is to evaluate the anti-inflammatory effects of ethyl acetate extracts (YYHs) of E. sagittatum and its mechanisms of action.
30424767	15	89	theme	TLR4/MD-2-mediated	1856:1873	arg1	pathway					1881:1887	the TLR4/MD-2-mediated NF-κB pathway	1852:1887	the TLR4/MD-2-mediated NF-κB pathway	1852:1887	CONCLUSION In brief, YYHs contributed to the inhibition of LPS-induced inflammatory response through the TLR4/MD-2-mediated NF-κB pathway and may be a potential choice to combat inflammation diseases.
30424767	9	90	theme	TLR4/MD-2	1138:1146	arg1	complex					1148:1154	TLR4/MD-2 complex	1138:1154	TLR4/MD-2 complex	1138:1154	In addition, expression of p-p65 protein and TLR4/MD-2 complex was detected by western blots and flow cytometric, respectively.
30424767	8	91	link	enzyme-linked	1049:1061	arg1	ELISA					1085:1089	ELISA	1085:1089	ELISA	1085:1089	Production of tumor necrosis factor-alpha (TNF-α) and interleukin-2 (IL-2) were measured by enzyme-linked immunosorbent assays (ELISA).
30424767	8	91	link	enzyme-linked	1049:1061	arg1	assays					1077:1082	enzyme-linked immunosorbent assays	1049:1082	enzyme-linked immunosorbent assays (ELISA)	1049:1090	Production of tumor necrosis factor-alpha (TNF-α) and interleukin-2 (IL-2) were measured by enzyme-linked immunosorbent assays (ELISA).
30424767	14	92	theme	p65	1724:1726	arg1	translocation					1736:1748	NF-κB p65 nuclear translocation	1718:1748	NF-κB p65 nuclear translocation	1718:1748	Further research showed that YYHs significantly suppressed NF-κB p65 nuclear translocation.
31518801	8	0	theme	short	1191:1195	arg1	time					1197:1200	a short time	1189:1200	a short time	1189:1200	Hence, this surface system will be a very promising strategy for eliminating bacterial infection on biomedical device and implants safely and effectively within a short time.
31518801	6	1	theme	coating	786:792	arg1	%					849:849	98.66%	844:849	98.66%	844:849	in vitro results showed that the antibacterial efficiency of the coating on Staphylococcus aureus and Escherichia coli was 98.66% and 99.77% respectively, when the coating was irradiated by 660 nm visible light for 20 min.
31518801	6	1	theme	coating	786:792	arg1	efficiency					768:777	the antibacterial efficiency	750:777	the antibacterial efficiency of the coating on Staphylococcus aureus and Escherichia coli	750:838	in vitro results showed that the antibacterial efficiency of the coating on Staphylococcus aureus and Escherichia coli was 98.66% and 99.77% respectively, when the coating was irradiated by 660 nm visible light for 20 min.
31518801	4	2	theme	coating	642:648	arg1	property					626:633	the antibacterial property	608:633	the antibacterial property of the coating	608:648	The covering of CS made the composite coating positively charged to further enhance the antibacterial property of the coating.
31518801	6	3	dep	in	721:722	arg1	vitro					724:728	vitro	724:728	vitro	724:728	in vitro results showed that the antibacterial efficiency of the coating on Staphylococcus aureus and Escherichia coli was 98.66% and 99.77% respectively, when the coating was irradiated by 660 nm visible light for 20 min.
31518801	7	4	theme	Cell	944:947	arg1	tests					957:961	Cell culture tests	944:961	Cell culture tests	944:961	Cell culture tests showed that CS/Ag/MoS2-Ti had no adverse effects on cell growth.
31518801	8	5	theme	promising	1070:1078	arg1	system					1048:1053	this surface system	1035:1053	this surface system	1035:1053	Hence, this surface system will be a very promising strategy for eliminating bacterial infection on biomedical device and implants safely and effectively within a short time.
31518801	8	5	theme	promising	1070:1078	arg1	strategy					1080:1087	a very promising strategy	1063:1087	a very promising strategy for eliminating bacterial infection on biomedical device and implants	1063:1157	Hence, this surface system will be a very promising strategy for eliminating bacterial infection on biomedical device and implants safely and effectively within a short time.
31518801	3	6	from	transfer	389:396	arg1	MoS2					431:434	MoS2	431:434	MoS2	431:434	Ag/MoS2 exhibited high photocatalytic activity due to the rapid transfer of photo-inspired electrons from MoS2 to Ag NPs, resulting in higher yields of radical oxygen species (ROS) to kill bacteria.
31518801	6	7	theme	antibacterial	754:766	arg1	%					849:849	98.66%	844:849	98.66%	844:849	in vitro results showed that the antibacterial efficiency of the coating on Staphylococcus aureus and Escherichia coli was 98.66% and 99.77% respectively, when the coating was irradiated by 660 nm visible light for 20 min.
31518801	6	7	theme	antibacterial	754:766	arg1	efficiency					768:777	the antibacterial efficiency	750:777	the antibacterial efficiency of the coating on Staphylococcus aureus and Escherichia coli	750:838	in vitro results showed that the antibacterial efficiency of the coating on Staphylococcus aureus and Escherichia coli was 98.66% and 99.77% respectively, when the coating was irradiated by 660 nm visible light for 20 min.
31518801	6	8	theme	visible	918:924	arg1	light					926:930	660 nm visible light	911:930	660 nm visible light	911:930	in vitro results showed that the antibacterial efficiency of the coating on Staphylococcus aureus and Escherichia coli was 98.66% and 99.77% respectively, when the coating was irradiated by 660 nm visible light for 20 min.
31518801	2	9	theme	MoS2	156:159	arg1	coating					161:167	The chitosan (CS)-modified MoS2 coating	129:167	The chitosan (CS)-modified MoS2 coating loaded with silver nanoparticles (Ag NPs)	129:209	The chitosan (CS)-modified MoS2 coating loaded with silver nanoparticles (Ag NPs) was designed on the surface of titanium (Ti) to kill bacteria rapidly and efficiently under 660 nm visible light.
31518801	1	10	theme	serious	87:93	arg1	problem					95:101	a serious problem	85:101	a serious problem threatening human health	85:126	Bacterial infection is a serious problem threatening human health.
31518801	1	10	theme	serious	87:93	arg1	infection					72:80	Bacterial infection	62:80	Bacterial infection	62:80	Bacterial infection is a serious problem threatening human health.
31518801	3	11	theme	electrons	416:424	arg1	transfer					389:396	the rapid transfer	379:396	the rapid transfer of photo-inspired electrons from MoS2 to Ag NPs	379:444	Ag/MoS2 exhibited high photocatalytic activity due to the rapid transfer of photo-inspired electrons from MoS2 to Ag NPs, resulting in higher yields of radical oxygen species (ROS) to kill bacteria.
31518801	5	12	theme	certain	692:698	arg1	effect					713:718	a certain photothermal effect	690:718	a certain photothermal effect	690:718	In addition, CS/Ag/MoS2-Ti also showed a certain photothermal effect.
31518801	2	13	theme	-modified	146:154	arg1	coating					161:167	The chitosan (CS)-modified MoS2 coating	129:167	The chitosan (CS)-modified MoS2 coating loaded with silver nanoparticles (Ag NPs)	129:209	The chitosan (CS)-modified MoS2 coating loaded with silver nanoparticles (Ag NPs) was designed on the surface of titanium (Ti) to kill bacteria rapidly and efficiently under 660 nm visible light.
31518801	0	14	theme	Photo-responsive	0:15	arg1	chitosan/Ag/MoS2					17:32	Photo-responsive chitosan/Ag/MoS2	0:32	Photo-responsive chitosan/Ag/MoS2 for rapid bacteria-killing	0:59	Photo-responsive chitosan/Ag/MoS2 for rapid bacteria-killing.
31518801	8	15	theme	bacterial	1105:1113	arg1	infection					1115:1123	bacterial infection	1105:1123	bacterial infection	1105:1123	Hence, this surface system will be a very promising strategy for eliminating bacterial infection on biomedical device and implants safely and effectively within a short time.
31518801	6	16	from	efficiency	768:777	arg1	aureus					812:817	Staphylococcus aureus	797:817	Staphylococcus aureus	797:817	in vitro results showed that the antibacterial efficiency of the coating on Staphylococcus aureus and Escherichia coli was 98.66% and 99.77% respectively, when the coating was irradiated by 660 nm visible light for 20 min.
31518801	6	16	from	efficiency	768:777	arg1	coli					835:838	Escherichia coli	823:838	Escherichia coli	823:838	in vitro results showed that the antibacterial efficiency of the coating on Staphylococcus aureus and Escherichia coli was 98.66% and 99.77% respectively, when the coating was irradiated by 660 nm visible light for 20 min.
31518801	3	17	theme	due	372:374	arg1	activity					363:370	high photocatalytic activity	343:370	high photocatalytic activity due to the rapid transfer of photo-inspired electrons from MoS2 to Ag NPs	343:444	Ag/MoS2 exhibited high photocatalytic activity due to the rapid transfer of photo-inspired electrons from MoS2 to Ag NPs, resulting in higher yields of radical oxygen species (ROS) to kill bacteria.
31518801	6	18	theme	nm	915:916	arg1	light					926:930	660 nm visible light	911:930	660 nm visible light	911:930	in vitro results showed that the antibacterial efficiency of the coating on Staphylococcus aureus and Escherichia coli was 98.66% and 99.77% respectively, when the coating was irradiated by 660 nm visible light for 20 min.
31518801	7	19	theme	cell	1015:1018	arg1	growth					1020:1025	cell growth	1015:1025	cell growth	1015:1025	Cell culture tests showed that CS/Ag/MoS2-Ti had no adverse effects on cell growth.
31518801	1	20	theme	human	115:119	arg1	health					121:126	human health	115:126	human health	115:126	Bacterial infection is a serious problem threatening human health.
31518801	3	21	theme	photo-inspired	401:414	arg1	electrons					416:424	photo-inspired electrons	401:424	photo-inspired electrons from MoS2	401:434	Ag/MoS2 exhibited high photocatalytic activity due to the rapid transfer of photo-inspired electrons from MoS2 to Ag NPs, resulting in higher yields of radical oxygen species (ROS) to kill bacteria.
31518801	3	22	theme	high	343:346	arg1	activity					363:370	high photocatalytic activity	343:370	high photocatalytic activity due to the rapid transfer of photo-inspired electrons from MoS2 to Ag NPs	343:444	Ag/MoS2 exhibited high photocatalytic activity due to the rapid transfer of photo-inspired electrons from MoS2 to Ag NPs, resulting in higher yields of radical oxygen species (ROS) to kill bacteria.
31518801	6	23	theme	660	911:913	arg1	nm					915:916	nm	915:916	nm	915:916	in vitro results showed that the antibacterial efficiency of the coating on Staphylococcus aureus and Escherichia coli was 98.66% and 99.77% respectively, when the coating was irradiated by 660 nm visible light for 20 min.
31518801	7	24	theme	adverse	996:1002	arg1	effects					1004:1010	no adverse effects	993:1010	no adverse effects	993:1010	Cell culture tests showed that CS/Ag/MoS2-Ti had no adverse effects on cell growth.
31518801	7	25	contain	had	989:991	arg2	effects					1004:1010	no adverse effects	993:1010	no adverse effects	993:1010	Cell culture tests showed that CS/Ag/MoS2-Ti had no adverse effects on cell growth.
31518801	7	25	contain	had	989:991	arg1	CS/Ag/MoS2-Ti					975:987	CS/Ag/MoS2-Ti	975:987	CS/Ag/MoS2-Ti	975:987	Cell culture tests showed that CS/Ag/MoS2-Ti had no adverse effects on cell growth.
31518801	3	26	theme	Ag	439:440	arg1	NPs					442:444	Ag NPs	439:444	Ag NPs	439:444	Ag/MoS2 exhibited high photocatalytic activity due to the rapid transfer of photo-inspired electrons from MoS2 to Ag NPs, resulting in higher yields of radical oxygen species (ROS) to kill bacteria.
31518801	3	27	theme	photocatalytic	348:361	arg1	activity					363:370	high photocatalytic activity	343:370	high photocatalytic activity due to the rapid transfer of photo-inspired electrons from MoS2 to Ag NPs	343:444	Ag/MoS2 exhibited high photocatalytic activity due to the rapid transfer of photo-inspired electrons from MoS2 to Ag NPs, resulting in higher yields of radical oxygen species (ROS) to kill bacteria.
31518801	3	28	theme	species	492:498	arg1	yields					467:472	higher yields	460:472	higher yields of radical oxygen species (ROS) to kill bacteria	460:521	Ag/MoS2 exhibited high photocatalytic activity due to the rapid transfer of photo-inspired electrons from MoS2 to Ag NPs, resulting in higher yields of radical oxygen species (ROS) to kill bacteria.
31518801	3	29	theme	rapid	383:387	arg1	transfer					389:396	the rapid transfer	379:396	the rapid transfer of photo-inspired electrons from MoS2 to Ag NPs	379:444	Ag/MoS2 exhibited high photocatalytic activity due to the rapid transfer of photo-inspired electrons from MoS2 to Ag NPs, resulting in higher yields of radical oxygen species (ROS) to kill bacteria.
31518801	3	30	theme	higher	460:465	arg1	yields					467:472	higher yields	460:472	higher yields of radical oxygen species (ROS) to kill bacteria	460:521	Ag/MoS2 exhibited high photocatalytic activity due to the rapid transfer of photo-inspired electrons from MoS2 to Ag NPs, resulting in higher yields of radical oxygen species (ROS) to kill bacteria.
31518801	2	31	theme	silver	181:186	arg1	nanoparticles					188:200	silver nanoparticles	181:200	silver nanoparticles (Ag NPs)	181:209	The chitosan (CS)-modified MoS2 coating loaded with silver nanoparticles (Ag NPs) was designed on the surface of titanium (Ti) to kill bacteria rapidly and efficiently under 660 nm visible light.
31518801	2	31	theme	silver	181:186	arg1	NPs					206:208	Ag NPs	203:208	Ag NPs	203:208	The chitosan (CS)-modified MoS2 coating loaded with silver nanoparticles (Ag NPs) was designed on the surface of titanium (Ti) to kill bacteria rapidly and efficiently under 660 nm visible light.
31518801	4	32	theme	antibacterial	612:624	arg1	property					626:633	the antibacterial property	608:633	the antibacterial property of the coating	608:648	The covering of CS made the composite coating positively charged to further enhance the antibacterial property of the coating.
31518801	8	33	theme	biomedical	1128:1137	arg1	device					1139:1144	biomedical device	1128:1144	biomedical device	1128:1144	Hence, this surface system will be a very promising strategy for eliminating bacterial infection on biomedical device and implants safely and effectively within a short time.
31518801	6	34	theme	in	721:722	arg1	results					730:736	in vitro results	721:736	in vitro results	721:736	in vitro results showed that the antibacterial efficiency of the coating on Staphylococcus aureus and Escherichia coli was 98.66% and 99.77% respectively, when the coating was irradiated by 660 nm visible light for 20 min.
31518801	0	35	theme	rapid	38:42	arg1	bacteria-killing					44:59	rapid bacteria-killing	38:59	rapid bacteria-killing	38:59	Photo-responsive chitosan/Ag/MoS2 for rapid bacteria-killing.
31518801	3	36	from	MoS2	431:434	arg1	electrons					416:424	photo-inspired electrons	401:424	photo-inspired electrons from MoS2	401:434	Ag/MoS2 exhibited high photocatalytic activity due to the rapid transfer of photo-inspired electrons from MoS2 to Ag NPs, resulting in higher yields of radical oxygen species (ROS) to kill bacteria.
31518801	3	36	from	MoS2	431:434	arg1	transfer					389:396	the rapid transfer	379:396	the rapid transfer of photo-inspired electrons from MoS2 to Ag NPs	379:444	Ag/MoS2 exhibited high photocatalytic activity due to the rapid transfer of photo-inspired electrons from MoS2 to Ag NPs, resulting in higher yields of radical oxygen species (ROS) to kill bacteria.
31518801	7	37	theme	culture	949:955	arg1	tests					957:961	Cell culture tests	944:961	Cell culture tests	944:961	Cell culture tests showed that CS/Ag/MoS2-Ti had no adverse effects on cell growth.
31518801	2	38	theme	visible	310:316	arg1	light					318:322	660 nm visible light	303:322	660 nm visible light	303:322	The chitosan (CS)-modified MoS2 coating loaded with silver nanoparticles (Ag NPs) was designed on the surface of titanium (Ti) to kill bacteria rapidly and efficiently under 660 nm visible light.
31518801	2	39	theme	nm	307:308	arg1	light					318:322	660 nm visible light	303:322	660 nm visible light	303:322	The chitosan (CS)-modified MoS2 coating loaded with silver nanoparticles (Ag NPs) was designed on the surface of titanium (Ti) to kill bacteria rapidly and efficiently under 660 nm visible light.
31518801	8	40	theme	surface	1040:1046	arg1	system					1048:1053	this surface system	1035:1053	this surface system	1035:1053	Hence, this surface system will be a very promising strategy for eliminating bacterial infection on biomedical device and implants safely and effectively within a short time.
31518801	8	40	theme	surface	1040:1046	arg1	strategy					1080:1087	a very promising strategy	1063:1087	a very promising strategy for eliminating bacterial infection on biomedical device and implants	1063:1157	Hence, this surface system will be a very promising strategy for eliminating bacterial infection on biomedical device and implants safely and effectively within a short time.
31518801	2	41	theme	660	303:305	arg1	nm					307:308	nm	307:308	nm	307:308	The chitosan (CS)-modified MoS2 coating loaded with silver nanoparticles (Ag NPs) was designed on the surface of titanium (Ti) to kill bacteria rapidly and efficiently under 660 nm visible light.
31518801	2	42	theme	Ag	203:204	arg1	nanoparticles					188:200	silver nanoparticles	181:200	silver nanoparticles (Ag NPs)	181:209	The chitosan (CS)-modified MoS2 coating loaded with silver nanoparticles (Ag NPs) was designed on the surface of titanium (Ti) to kill bacteria rapidly and efficiently under 660 nm visible light.
31518801	2	42	theme	Ag	203:204	arg1	NPs					206:208	Ag NPs	203:208	Ag NPs	203:208	The chitosan (CS)-modified MoS2 coating loaded with silver nanoparticles (Ag NPs) was designed on the surface of titanium (Ti) to kill bacteria rapidly and efficiently under 660 nm visible light.
31518801	2	43	theme	titanium	242:249	arg1	surface					231:237	the surface	227:237	the surface of titanium (Ti)	227:254	The chitosan (CS)-modified MoS2 coating loaded with silver nanoparticles (Ag NPs) was designed on the surface of titanium (Ti) to kill bacteria rapidly and efficiently under 660 nm visible light.
31518801	5	44	theme	photothermal	700:711	arg1	effect					713:718	a certain photothermal effect	690:718	a certain photothermal effect	690:718	In addition, CS/Ag/MoS2-Ti also showed a certain photothermal effect.
31518801	1	45	theme	Bacterial	62:70	arg1	problem					95:101	a serious problem	85:101	a serious problem threatening human health	85:126	Bacterial infection is a serious problem threatening human health.
31518801	1	45	theme	Bacterial	62:70	arg1	infection					72:80	Bacterial infection	62:80	Bacterial infection	62:80	Bacterial infection is a serious problem threatening human health.
31518801	4	46	theme	charged	581:587	arg1	coating					562:568	the composite coating	548:568	the composite coating positively charged	548:587	The covering of CS made the composite coating positively charged to further enhance the antibacterial property of the coating.
31518801	3	47	theme	radical	477:483	arg1	ROS					501:503	ROS	501:503	ROS	501:503	Ag/MoS2 exhibited high photocatalytic activity due to the rapid transfer of photo-inspired electrons from MoS2 to Ag NPs, resulting in higher yields of radical oxygen species (ROS) to kill bacteria.
31518801	3	47	theme	radical	477:483	arg1	species					492:498	radical oxygen species	477:498	radical oxygen species (ROS) to kill bacteria	477:521	Ag/MoS2 exhibited high photocatalytic activity due to the rapid transfer of photo-inspired electrons from MoS2 to Ag NPs, resulting in higher yields of radical oxygen species (ROS) to kill bacteria.
31518801	3	48	theme	oxygen	485:490	arg1	ROS					501:503	ROS	501:503	ROS	501:503	Ag/MoS2 exhibited high photocatalytic activity due to the rapid transfer of photo-inspired electrons from MoS2 to Ag NPs, resulting in higher yields of radical oxygen species (ROS) to kill bacteria.
31518801	3	48	theme	oxygen	485:490	arg1	species					492:498	radical oxygen species	477:498	radical oxygen species (ROS) to kill bacteria	477:521	Ag/MoS2 exhibited high photocatalytic activity due to the rapid transfer of photo-inspired electrons from MoS2 to Ag NPs, resulting in higher yields of radical oxygen species (ROS) to kill bacteria.
31518801	4	49	theme	composite	552:560	arg1	coating					562:568	the composite coating	548:568	the composite coating positively charged	548:587	The covering of CS made the composite coating positively charged to further enhance the antibacterial property of the coating.
30628301	8	0	theme	various	1209:1215	arg1	biosynthesis					1245:1256	biosynthesis	1245:1256	biosynthesis of other secondary metabolites	1245:1287	Our results reveal a wide genetic diversity of organisms involved in various essential processes such as biosynthesis of other secondary metabolites, transcription, Glycan biosynthesis and metabolism, cell growth and death, and carbohydrate metabolism.
30628301	8	0	theme	various	1209:1215	arg1	transcription					1290:1302	transcription	1290:1302	transcription	1290:1302	Our results reveal a wide genetic diversity of organisms involved in various essential processes such as biosynthesis of other secondary metabolites, transcription, Glycan biosynthesis and metabolism, cell growth and death, and carbohydrate metabolism.
30628301	8	0	theme	various	1209:1215	arg1	metabolism					1381:1390	carbohydrate metabolism	1368:1390	carbohydrate metabolism	1368:1390	Our results reveal a wide genetic diversity of organisms involved in various essential processes such as biosynthesis of other secondary metabolites, transcription, Glycan biosynthesis and metabolism, cell growth and death, and carbohydrate metabolism.
30628301	8	0	theme	various	1209:1215	arg1	growth					1346:1351	cell growth	1341:1351	cell growth	1341:1351	Our results reveal a wide genetic diversity of organisms involved in various essential processes such as biosynthesis of other secondary metabolites, transcription, Glycan biosynthesis and metabolism, cell growth and death, and carbohydrate metabolism.
30628301	8	0	theme	various	1209:1215	arg1	biosynthesis					1312:1323	Glycan biosynthesis	1305:1323	Glycan biosynthesis	1305:1323	Our results reveal a wide genetic diversity of organisms involved in various essential processes such as biosynthesis of other secondary metabolites, transcription, Glycan biosynthesis and metabolism, cell growth and death, and carbohydrate metabolism.
30628301	8	0	theme	various	1209:1215	arg1	processes					1227:1235	various essential processes	1209:1235	various essential processes such as biosynthesis of other secondary metabolites, transcription, Glycan biosynthesis and metabolism, cell growth and death, and carbohydrate metabolism	1209:1390	Our results reveal a wide genetic diversity of organisms involved in various essential processes such as biosynthesis of other secondary metabolites, transcription, Glycan biosynthesis and metabolism, cell growth and death, and carbohydrate metabolism.
30628301	2	1	theme	rhizosphere	287:297	arg1	communities					299:309	rhizosphere communities	287:309	rhizosphere communities	287:309	To our knowledge, the compositions and functions of rhizosphere communities in riparian buffer strips have not been reported.
30628301	10	2	theme	water	1813:1817	arg1	protection					1833:1842	water environmental protection	1813:1842	water environmental protection	1813:1842	We analyzed the differences of different plants from the perspective of bacterial community composition and function and provide a foundation for vegetation construction and water environmental protection in riparian buffer strips of the Danjiangkou Reservoir.
30628301	9	3	theme	plants	1489:1494	arg1	follows					1539:1545	follows	1539:1545	follows	1539:1545	Based on the 16S rRNA gene copy number of the detected phylotype, the bacterial rhizospheres of plants in riparian buffer strips can be ranked as follows:Discocleidion rufescens > Phragmites australis > Vetiveria zizanioides > Pyrus betulifolia.
30628301	9	3	theme	plants	1489:1494	arg1	rhizospheres					1473:1484	the bacterial rhizospheres	1459:1484	the bacterial rhizospheres of plants in riparian buffer strips	1459:1520	Based on the 16S rRNA gene copy number of the detected phylotype, the bacterial rhizospheres of plants in riparian buffer strips can be ranked as follows:Discocleidion rufescens > Phragmites australis > Vetiveria zizanioides > Pyrus betulifolia.
30628301	2	4	theme	buffer	323:328	arg1	strips					330:335	riparian buffer strips	314:335	riparian buffer strips	314:335	To our knowledge, the compositions and functions of rhizosphere communities in riparian buffer strips have not been reported.
30628301	9	5	theme	riparian	1499:1506	arg1	strips					1515:1520	riparian buffer strips	1499:1520	riparian buffer strips	1499:1520	Based on the 16S rRNA gene copy number of the detected phylotype, the bacterial rhizospheres of plants in riparian buffer strips can be ranked as follows:Discocleidion rufescens > Phragmites australis > Vetiveria zizanioides > Pyrus betulifolia.
30628301	10	6	from	construction	1796:1807	arg1	strips					1863:1868	riparian buffer strips	1847:1868	riparian buffer strips of the Danjiangkou Reservoir	1847:1897	We analyzed the differences of different plants from the perspective of bacterial community composition and function and provide a foundation for vegetation construction and water environmental protection in riparian buffer strips of the Danjiangkou Reservoir.
30628301	0	7	theme	Reservoir	130:138	arg1	China					141:145	the Danjiangkou Reservoir, China]	114:146	China	141:145	[Composition and Predictive Functional Analysis of Rhizosphere Bacterial Communities in Riparian Buffer Strips in the Danjiangkou Reservoir, China].
30628301	10	8	theme	riparian	1847:1854	arg1	strips					1863:1868	riparian buffer strips	1847:1868	riparian buffer strips of the Danjiangkou Reservoir	1847:1897	We analyzed the differences of different plants from the perspective of bacterial community composition and function and provide a foundation for vegetation construction and water environmental protection in riparian buffer strips of the Danjiangkou Reservoir.
30628301	1	9	theme	biogeochemical	204:217	arg1	cycle					228:232	the global biogeochemical nitrogen cycle	193:232	the global biogeochemical nitrogen cycle	193:232	Microbial communities play crucial roles in the global biogeochemical nitrogen cycle.
30628301	8	10	theme	secondary	1267:1275	arg1	metabolites					1277:1287	other secondary metabolites	1261:1287	other secondary metabolites	1261:1287	Our results reveal a wide genetic diversity of organisms involved in various essential processes such as biosynthesis of other secondary metabolites, transcription, Glycan biosynthesis and metabolism, cell growth and death, and carbohydrate metabolism.
30628301	4	11	theme	community	640:648	arg1	structure					650:658	community structure	640:658	community structure	640:658	High-throughput sequencing was performed to analyze the community structure and diversity of bacteria.
30628301	10	12	theme	plants	1680:1685	arg1	differences					1655:1665	the differences	1651:1665	the differences of different plants from the perspective of bacterial community composition and function	1651:1754	We analyzed the differences of different plants from the perspective of bacterial community composition and function and provide a foundation for vegetation construction and water environmental protection in riparian buffer strips of the Danjiangkou Reservoir.
30628301	5	13	theme	rDNA	722:725	arg1	sequences					727:735	16S rDNA sequences	718:735	16S rDNA sequences	718:735	Phylogenetic analysis based on 16S rDNA sequences shows that the bacterial communities can be divided into 31 major phylogenetic groups.
30628301	10	14	theme	Reservoir	1889:1897	arg1	strips					1863:1868	riparian buffer strips	1847:1868	riparian buffer strips of the Danjiangkou Reservoir	1847:1897	We analyzed the differences of different plants from the perspective of bacterial community composition and function and provide a foundation for vegetation construction and water environmental protection in riparian buffer strips of the Danjiangkou Reservoir.
30628301	5	15	theme	Phylogenetic	687:698	arg1	analysis					700:707	Phylogenetic analysis	687:707	Phylogenetic analysis based on 16S rDNA sequences	687:735	Phylogenetic analysis based on 16S rDNA sequences shows that the bacterial communities can be divided into 31 major phylogenetic groups.
30628301	3	16	dep	Discocleidion	516:528	arg1	rufescens					530:538	Discocleidion rufescens	516:538	Discocleidion rufescens	516:538	In this study, rhizosphere soil samples were collected from herbs (Vetiveria zizanioides and Phragmites australis), trees (Pyrus betulifolia), and shrubs (Discocleidion rufescens) in the Danjiangkou Reservoir in June 2017.
30628301	0	17	from	Strips	104:109	arg1	China					141:145	the Danjiangkou Reservoir, China]	114:146	China	141:145	[Composition and Predictive Functional Analysis of Rhizosphere Bacterial Communities in Riparian Buffer Strips in the Danjiangkou Reservoir, China].
30628301	8	18	theme	carbohydrate	1368:1379	arg1	metabolism					1381:1390	carbohydrate metabolism	1368:1390	carbohydrate metabolism	1368:1390	Our results reveal a wide genetic diversity of organisms involved in various essential processes such as biosynthesis of other secondary metabolites, transcription, Glycan biosynthesis and metabolism, cell growth and death, and carbohydrate metabolism.
30628301	2	19	from	functions	274:282	arg1	strips					330:335	riparian buffer strips	314:335	riparian buffer strips	314:335	To our knowledge, the compositions and functions of rhizosphere communities in riparian buffer strips have not been reported.
30628301	0	20	theme	Communities	73:83	arg1	[Composition					0:11	[Composition	0:11	[Composition	0:11	[Composition and Predictive Functional Analysis of Rhizosphere Bacterial Communities in Riparian Buffer Strips in the Danjiangkou Reservoir, China].
30628301	0	20	theme	Communities	73:83	arg1	Analysis					39:46	Predictive Functional Analysis	17:46	Predictive Functional Analysis	17:46	[Composition and Predictive Functional Analysis of Rhizosphere Bacterial Communities in Riparian Buffer Strips in the Danjiangkou Reservoir, China].
30628301	1	21	theme	Microbial	149:157	arg1	communities					159:169	Microbial communities	149:169	Microbial communities	149:169	Microbial communities play crucial roles in the global biogeochemical nitrogen cycle.
30628301	7	22	used	used	1044:1047	arg2	investigation					962:974	Phylogenetic investigation	949:974	Phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt)	949:1038	Phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) was used to determine the metabolic and functional abilities of the observed bacterial communities.
30628301	0	23	theme	Riparian	88:95	arg1	Strips					104:109	Riparian Buffer Strips	88:109	Riparian Buffer Strips in the Danjiangkou Reservoir, China]	88:146	[Composition and Predictive Functional Analysis of Rhizosphere Bacterial Communities in Riparian Buffer Strips in the Danjiangkou Reservoir, China].
30628301	7	24	theme	unobserved	1012:1021	arg1	PICRUSt					1031:1037	PICRUSt	1031:1037	PICRUSt	1031:1037	Phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) was used to determine the metabolic and functional abilities of the observed bacterial communities.
30628301	7	24	theme	unobserved	1012:1021	arg1	states					1023:1028	unobserved states	1012:1028	unobserved states (PICRUSt)	1012:1038	Phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) was used to determine the metabolic and functional abilities of the observed bacterial communities.
30628301	0	25	from	[Composition	0:11	arg1	Strips					104:109	Riparian Buffer Strips	88:109	Riparian Buffer Strips in the Danjiangkou Reservoir, China]	88:146	[Composition and Predictive Functional Analysis of Rhizosphere Bacterial Communities in Riparian Buffer Strips in the Danjiangkou Reservoir, China].
30628301	9	26	dep	follows	1539:1545	arg1	Discocleidion					1547:1559	Discocleidion	1547:1559	Discocleidion	1547:1559	Based on the 16S rRNA gene copy number of the detected phylotype, the bacterial rhizospheres of plants in riparian buffer strips can be ranked as follows:Discocleidion rufescens > Phragmites australis > Vetiveria zizanioides > Pyrus betulifolia.
30628301	10	27	theme	bacterial	1711:1719	arg1	composition					1731:1741	bacterial community composition	1711:1741	bacterial community composition	1711:1741	We analyzed the differences of different plants from the perspective of bacterial community composition and function and provide a foundation for vegetation construction and water environmental protection in riparian buffer strips of the Danjiangkou Reservoir.
30628301	7	28	theme	communities	979:989	arg1	investigation					962:974	Phylogenetic investigation	949:974	Phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt)	949:1038	Phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) was used to determine the metabolic and functional abilities of the observed bacterial communities.
30628301	3	29	theme	soil	388:391	arg1	samples					393:399	rhizosphere soil samples	376:399	rhizosphere soil samples	376:399	In this study, rhizosphere soil samples were collected from herbs (Vetiveria zizanioides and Phragmites australis), trees (Pyrus betulifolia), and shrubs (Discocleidion rufescens) in the Danjiangkou Reservoir in June 2017.
30628301	0	30	from	Analysis	39:46	arg1	Strips					104:109	Riparian Buffer Strips	88:109	Riparian Buffer Strips in the Danjiangkou Reservoir, China]	88:146	[Composition and Predictive Functional Analysis of Rhizosphere Bacterial Communities in Riparian Buffer Strips in the Danjiangkou Reservoir, China].
30628301	9	31	dep	Discocleidion	1547:1559	arg1	rufescens					1561:1569	Discocleidion rufescens > Phragmites australis	1547:1592	follows:Discocleidion rufescens > Phragmites australis > Vetiveria zizanioides > Pyrus betulifolia	1539:1636	Based on the 16S rRNA gene copy number of the detected phylotype, the bacterial rhizospheres of plants in riparian buffer strips can be ranked as follows:Discocleidion rufescens > Phragmites australis > Vetiveria zizanioides > Pyrus betulifolia.
30628301	9	31	dep	Discocleidion	1547:1559	arg1	betulifolia					1626:1636	betulifolia	1626:1636	betulifolia	1626:1636	Based on the 16S rRNA gene copy number of the detected phylotype, the bacterial rhizospheres of plants in riparian buffer strips can be ranked as follows:Discocleidion rufescens > Phragmites australis > Vetiveria zizanioides > Pyrus betulifolia.
30628301	9	31	dep	Discocleidion	1547:1559	arg1	Phragmites					1573:1582	Phragmites	1573:1582	Phragmites	1573:1582	Based on the 16S rRNA gene copy number of the detected phylotype, the bacterial rhizospheres of plants in riparian buffer strips can be ranked as follows:Discocleidion rufescens > Phragmites australis > Vetiveria zizanioides > Pyrus betulifolia.
30628301	10	32	theme	function	1747:1754	arg1	perspective					1696:1706	the perspective	1692:1706	the perspective of bacterial community composition and function	1692:1754	We analyzed the differences of different plants from the perspective of bacterial community composition and function and provide a foundation for vegetation construction and water environmental protection in riparian buffer strips of the Danjiangkou Reservoir.
30628301	0	33	theme	Predictive	17:26	arg1	Analysis					39:46	Predictive Functional Analysis	17:46	Predictive Functional Analysis	17:46	[Composition and Predictive Functional Analysis of Rhizosphere Bacterial Communities in Riparian Buffer Strips in the Danjiangkou Reservoir, China].
30628301	9	34	from	rhizospheres	1473:1484	arg1	strips					1515:1520	riparian buffer strips	1499:1520	riparian buffer strips	1499:1520	Based on the 16S rRNA gene copy number of the detected phylotype, the bacterial rhizospheres of plants in riparian buffer strips can be ranked as follows:Discocleidion rufescens > Phragmites australis > Vetiveria zizanioides > Pyrus betulifolia.
30628301	3	35	from	Reservoir	560:568	arg1	June					573:576	June	573:576	June	573:576	In this study, rhizosphere soil samples were collected from herbs (Vetiveria zizanioides and Phragmites australis), trees (Pyrus betulifolia), and shrubs (Discocleidion rufescens) in the Danjiangkou Reservoir in June 2017.
30628301	10	36	theme	composition	1731:1741	arg1	perspective					1696:1706	the perspective	1692:1706	the perspective of bacterial community composition and function	1692:1754	We analyzed the differences of different plants from the perspective of bacterial community composition and function and provide a foundation for vegetation construction and water environmental protection in riparian buffer strips of the Danjiangkou Reservoir.
30628301	9	37	theme	rRNA	1410:1413	arg1	number					1425:1430	the 16S rRNA gene copy number	1402:1430	the 16S rRNA gene copy number of the detected phylotype	1402:1456	Based on the 16S rRNA gene copy number of the detected phylotype, the bacterial rhizospheres of plants in riparian buffer strips can be ranked as follows:Discocleidion rufescens > Phragmites australis > Vetiveria zizanioides > Pyrus betulifolia.
30628301	10	38	from	perspective	1696:1706	arg1	differences					1655:1665	the differences	1651:1665	the differences of different plants from the perspective of bacterial community composition and function	1651:1754	We analyzed the differences of different plants from the perspective of bacterial community composition and function and provide a foundation for vegetation construction and water environmental protection in riparian buffer strips of the Danjiangkou Reservoir.
30628301	10	38	from	perspective	1696:1706	arg1	plants					1680:1685	different plants	1670:1685	different plants from the perspective of bacterial community composition and function	1670:1754	We analyzed the differences of different plants from the perspective of bacterial community composition and function and provide a foundation for vegetation construction and water environmental protection in riparian buffer strips of the Danjiangkou Reservoir.
30628301	5	39	theme	major	797:801	arg1	groups					816:821	31 major phylogenetic groups	794:821	31 major phylogenetic groups	794:821	Phylogenetic analysis based on 16S rDNA sequences shows that the bacterial communities can be divided into 31 major phylogenetic groups.
30628301	9	40	theme	copy	1420:1423	arg1	number					1425:1430	the 16S rRNA gene copy number	1402:1430	the 16S rRNA gene copy number of the detected phylotype	1402:1456	Based on the 16S rRNA gene copy number of the detected phylotype, the bacterial rhizospheres of plants in riparian buffer strips can be ranked as follows:Discocleidion rufescens > Phragmites australis > Vetiveria zizanioides > Pyrus betulifolia.
30628301	7	41	theme	Phylogenetic	949:960	arg1	investigation					962:974	Phylogenetic investigation	949:974	Phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt)	949:1038	Phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) was used to determine the metabolic and functional abilities of the observed bacterial communities.
30628301	8	42	theme	wide	1161:1164	arg1	diversity					1174:1182	a wide genetic diversity	1159:1182	a wide genetic diversity of organisms involved in various essential processes such as biosynthesis of other secondary metabolites, transcription, Glycan biosynthesis and metabolism, cell growth and death, and carbohydrate metabolism	1159:1390	Our results reveal a wide genetic diversity of organisms involved in various essential processes such as biosynthesis of other secondary metabolites, transcription, Glycan biosynthesis and metabolism, cell growth and death, and carbohydrate metabolism.
30628301	6	43	theme	phylogenetic	837:848	arg1	groups					850:855	The dominant phylogenetic groups	824:855	The dominant phylogenetic groups	824:855	The dominant phylogenetic groups include Proteobacteria, Bacteroidetes, Actinobacteria, Gemmatimonadetes, and Acidobacteria.
30628301	4	44	theme	High-throughput	584:598	arg1	sequencing					600:609	High-throughput sequencing	584:609	High-throughput sequencing	584:609	High-throughput sequencing was performed to analyze the community structure and diversity of bacteria.
30628301	9	45	theme	detected	1439:1446	arg1	phylotype					1448:1456	the detected phylotype	1435:1456	the detected phylotype	1435:1456	Based on the 16S rRNA gene copy number of the detected phylotype, the bacterial rhizospheres of plants in riparian buffer strips can be ranked as follows:Discocleidion rufescens > Phragmites australis > Vetiveria zizanioides > Pyrus betulifolia.
30628301	4	46	dep	structure	650:658	arg1	the					636:638	the	636:638	the	636:638	High-throughput sequencing was performed to analyze the community structure and diversity of bacteria.
30628301	10	47	theme	vegetation	1785:1794	arg1	construction					1796:1807	vegetation construction	1785:1807	vegetation construction	1785:1807	We analyzed the differences of different plants from the perspective of bacterial community composition and function and provide a foundation for vegetation construction and water environmental protection in riparian buffer strips of the Danjiangkou Reservoir.
30628301	8	48	theme	organisms	1187:1195	arg1	diversity					1174:1182	a wide genetic diversity	1159:1182	a wide genetic diversity of organisms involved in various essential processes such as biosynthesis of other secondary metabolites, transcription, Glycan biosynthesis and metabolism, cell growth and death, and carbohydrate metabolism	1159:1390	Our results reveal a wide genetic diversity of organisms involved in various essential processes such as biosynthesis of other secondary metabolites, transcription, Glycan biosynthesis and metabolism, cell growth and death, and carbohydrate metabolism.
30628301	10	49	from	differences	1655:1665	arg1	perspective					1696:1706	the perspective	1692:1706	the perspective of bacterial community composition and function	1692:1754	We analyzed the differences of different plants from the perspective of bacterial community composition and function and provide a foundation for vegetation construction and water environmental protection in riparian buffer strips of the Danjiangkou Reservoir.
30628301	2	50	theme	communities	299:309	arg1	functions					274:282	functions	274:282	functions	274:282	To our knowledge, the compositions and functions of rhizosphere communities in riparian buffer strips have not been reported.
30628301	2	50	theme	communities	299:309	arg1	compositions					257:268	compositions	257:268	compositions	257:268	To our knowledge, the compositions and functions of rhizosphere communities in riparian buffer strips have not been reported.
30628301	9	51	theme	bacterial	1463:1471	arg1	follows					1539:1545	follows	1539:1545	follows	1539:1545	Based on the 16S rRNA gene copy number of the detected phylotype, the bacterial rhizospheres of plants in riparian buffer strips can be ranked as follows:Discocleidion rufescens > Phragmites australis > Vetiveria zizanioides > Pyrus betulifolia.
30628301	9	51	theme	bacterial	1463:1471	arg1	rhizospheres					1473:1484	the bacterial rhizospheres	1459:1484	the bacterial rhizospheres of plants in riparian buffer strips	1459:1520	Based on the 16S rRNA gene copy number of the detected phylotype, the bacterial rhizospheres of plants in riparian buffer strips can be ranked as follows:Discocleidion rufescens > Phragmites australis > Vetiveria zizanioides > Pyrus betulifolia.
30628301	10	52	theme	environmental	1819:1831	arg1	protection					1833:1842	water environmental protection	1813:1842	water environmental protection	1813:1842	We analyzed the differences of different plants from the perspective of bacterial community composition and function and provide a foundation for vegetation construction and water environmental protection in riparian buffer strips of the Danjiangkou Reservoir.
30628301	1	53	theme	crucial	176:182	arg1	roles					184:188	crucial roles	176:188	crucial roles	176:188	Microbial communities play crucial roles in the global biogeochemical nitrogen cycle.
30628301	8	54	theme	essential	1217:1225	arg1	biosynthesis					1245:1256	biosynthesis	1245:1256	biosynthesis of other secondary metabolites	1245:1287	Our results reveal a wide genetic diversity of organisms involved in various essential processes such as biosynthesis of other secondary metabolites, transcription, Glycan biosynthesis and metabolism, cell growth and death, and carbohydrate metabolism.
30628301	8	54	theme	essential	1217:1225	arg1	transcription					1290:1302	transcription	1290:1302	transcription	1290:1302	Our results reveal a wide genetic diversity of organisms involved in various essential processes such as biosynthesis of other secondary metabolites, transcription, Glycan biosynthesis and metabolism, cell growth and death, and carbohydrate metabolism.
30628301	8	54	theme	essential	1217:1225	arg1	metabolism					1381:1390	carbohydrate metabolism	1368:1390	carbohydrate metabolism	1368:1390	Our results reveal a wide genetic diversity of organisms involved in various essential processes such as biosynthesis of other secondary metabolites, transcription, Glycan biosynthesis and metabolism, cell growth and death, and carbohydrate metabolism.
30628301	8	54	theme	essential	1217:1225	arg1	growth					1346:1351	cell growth	1341:1351	cell growth	1341:1351	Our results reveal a wide genetic diversity of organisms involved in various essential processes such as biosynthesis of other secondary metabolites, transcription, Glycan biosynthesis and metabolism, cell growth and death, and carbohydrate metabolism.
30628301	8	54	theme	essential	1217:1225	arg1	biosynthesis					1312:1323	Glycan biosynthesis	1305:1323	Glycan biosynthesis	1305:1323	Our results reveal a wide genetic diversity of organisms involved in various essential processes such as biosynthesis of other secondary metabolites, transcription, Glycan biosynthesis and metabolism, cell growth and death, and carbohydrate metabolism.
30628301	8	54	theme	essential	1217:1225	arg1	processes					1227:1235	various essential processes	1209:1235	various essential processes such as biosynthesis of other secondary metabolites, transcription, Glycan biosynthesis and metabolism, cell growth and death, and carbohydrate metabolism	1209:1390	Our results reveal a wide genetic diversity of organisms involved in various essential processes such as biosynthesis of other secondary metabolites, transcription, Glycan biosynthesis and metabolism, cell growth and death, and carbohydrate metabolism.
30628301	7	55	theme	communities	1127:1137	arg1	abilities					1091:1099	the metabolic and functional abilities	1062:1099	the metabolic and functional abilities of the observed bacterial communities	1062:1137	Phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) was used to determine the metabolic and functional abilities of the observed bacterial communities.
30628301	3	56	theme	Danjiangkou	548:558	arg1	Reservoir					560:568	the Danjiangkou Reservoir	544:568	the Danjiangkou Reservoir in June 2017	544:581	In this study, rhizosphere soil samples were collected from herbs (Vetiveria zizanioides and Phragmites australis), trees (Pyrus betulifolia), and shrubs (Discocleidion rufescens) in the Danjiangkou Reservoir in June 2017.
30628301	0	57	theme	Rhizosphere	51:61	arg1	Communities					73:83	Rhizosphere Bacterial Communities	51:83	Rhizosphere Bacterial Communities	51:83	[Composition and Predictive Functional Analysis of Rhizosphere Bacterial Communities in Riparian Buffer Strips in the Danjiangkou Reservoir, China].
30628301	8	58	theme	Glycan	1305:1310	arg1	biosynthesis					1312:1323	Glycan biosynthesis	1305:1323	Glycan biosynthesis	1305:1323	Our results reveal a wide genetic diversity of organisms involved in various essential processes such as biosynthesis of other secondary metabolites, transcription, Glycan biosynthesis and metabolism, cell growth and death, and carbohydrate metabolism.
30628301	7	59	theme	observed	1108:1115	arg1	communities					1127:1137	the observed bacterial communities	1104:1137	the observed bacterial communities	1104:1137	Phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) was used to determine the metabolic and functional abilities of the observed bacterial communities.
30628301	2	60	theme	riparian	314:321	arg1	strips					330:335	riparian buffer strips	314:335	riparian buffer strips	314:335	To our knowledge, the compositions and functions of rhizosphere communities in riparian buffer strips have not been reported.
30628301	1	61	theme	global	197:202	arg1	cycle					228:232	the global biogeochemical nitrogen cycle	193:232	the global biogeochemical nitrogen cycle	193:232	Microbial communities play crucial roles in the global biogeochemical nitrogen cycle.
30628301	5	62	theme	16S	718:720	arg1	sequences					727:735	16S rDNA sequences	718:735	16S rDNA sequences	718:735	Phylogenetic analysis based on 16S rDNA sequences shows that the bacterial communities can be divided into 31 major phylogenetic groups.
30628301	2	63	dep	compositions	257:268	arg1	the					253:255	the	253:255	the	253:255	To our knowledge, the compositions and functions of rhizosphere communities in riparian buffer strips have not been reported.
30628301	1	64	theme	nitrogen	219:226	arg1	cycle					228:232	the global biogeochemical nitrogen cycle	193:232	the global biogeochemical nitrogen cycle	193:232	Microbial communities play crucial roles in the global biogeochemical nitrogen cycle.
30628301	8	65	theme	other	1261:1265	arg1	metabolites					1277:1287	other secondary metabolites	1261:1287	other secondary metabolites	1261:1287	Our results reveal a wide genetic diversity of organisms involved in various essential processes such as biosynthesis of other secondary metabolites, transcription, Glycan biosynthesis and metabolism, cell growth and death, and carbohydrate metabolism.
30628301	10	66	theme	different	1670:1678	arg1	plants					1680:1685	different plants	1670:1685	different plants from the perspective of bacterial community composition and function	1670:1754	We analyzed the differences of different plants from the perspective of bacterial community composition and function and provide a foundation for vegetation construction and water environmental protection in riparian buffer strips of the Danjiangkou Reservoir.
30628301	7	67	theme	functional	1080:1089	arg1	abilities					1091:1099	the metabolic and functional abilities	1062:1099	the metabolic and functional abilities of the observed bacterial communities	1062:1137	Phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) was used to determine the metabolic and functional abilities of the observed bacterial communities.
30628301	10	68	theme	Danjiangkou	1877:1887	arg1	Reservoir					1889:1897	the Danjiangkou Reservoir	1873:1897	the Danjiangkou Reservoir	1873:1897	We analyzed the differences of different plants from the perspective of bacterial community composition and function and provide a foundation for vegetation construction and water environmental protection in riparian buffer strips of the Danjiangkou Reservoir.
30628301	8	69	theme	metabolites	1277:1287	arg1	biosynthesis					1245:1256	biosynthesis	1245:1256	biosynthesis of other secondary metabolites	1245:1287	Our results reveal a wide genetic diversity of organisms involved in various essential processes such as biosynthesis of other secondary metabolites, transcription, Glycan biosynthesis and metabolism, cell growth and death, and carbohydrate metabolism.
30628301	8	69	theme	metabolites	1277:1287	arg1	transcription					1290:1302	transcription	1290:1302	transcription	1290:1302	Our results reveal a wide genetic diversity of organisms involved in various essential processes such as biosynthesis of other secondary metabolites, transcription, Glycan biosynthesis and metabolism, cell growth and death, and carbohydrate metabolism.
30628301	8	69	theme	metabolites	1277:1287	arg1	metabolism					1381:1390	carbohydrate metabolism	1368:1390	carbohydrate metabolism	1368:1390	Our results reveal a wide genetic diversity of organisms involved in various essential processes such as biosynthesis of other secondary metabolites, transcription, Glycan biosynthesis and metabolism, cell growth and death, and carbohydrate metabolism.
30628301	8	69	theme	metabolites	1277:1287	arg1	growth					1346:1351	cell growth	1341:1351	cell growth	1341:1351	Our results reveal a wide genetic diversity of organisms involved in various essential processes such as biosynthesis of other secondary metabolites, transcription, Glycan biosynthesis and metabolism, cell growth and death, and carbohydrate metabolism.
30628301	8	69	theme	metabolites	1277:1287	arg1	biosynthesis					1312:1323	Glycan biosynthesis	1305:1323	Glycan biosynthesis	1305:1323	Our results reveal a wide genetic diversity of organisms involved in various essential processes such as biosynthesis of other secondary metabolites, transcription, Glycan biosynthesis and metabolism, cell growth and death, and carbohydrate metabolism.
30628301	8	69	theme	metabolites	1277:1287	arg1	death					1357:1361	death	1357:1361	death	1357:1361	Our results reveal a wide genetic diversity of organisms involved in various essential processes such as biosynthesis of other secondary metabolites, transcription, Glycan biosynthesis and metabolism, cell growth and death, and carbohydrate metabolism.
30628301	8	69	theme	metabolites	1277:1287	arg1	metabolism					1329:1338	metabolism	1329:1338	metabolism	1329:1338	Our results reveal a wide genetic diversity of organisms involved in various essential processes such as biosynthesis of other secondary metabolites, transcription, Glycan biosynthesis and metabolism, cell growth and death, and carbohydrate metabolism.
30628301	7	70	theme	metabolic	1066:1074	arg1	abilities					1091:1099	the metabolic and functional abilities	1062:1099	the metabolic and functional abilities of the observed bacterial communities	1062:1137	Phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) was used to determine the metabolic and functional abilities of the observed bacterial communities.
30628301	10	71	theme	buffer	1856:1861	arg1	strips					1863:1868	riparian buffer strips	1847:1868	riparian buffer strips of the Danjiangkou Reservoir	1847:1897	We analyzed the differences of different plants from the perspective of bacterial community composition and function and provide a foundation for vegetation construction and water environmental protection in riparian buffer strips of the Danjiangkou Reservoir.
30628301	5	72	theme	bacterial	752:760	arg1	communities					762:772	the bacterial communities	748:772	the bacterial communities	748:772	Phylogenetic analysis based on 16S rDNA sequences shows that the bacterial communities can be divided into 31 major phylogenetic groups.
30628301	9	73	theme	buffer	1508:1513	arg1	strips					1515:1520	riparian buffer strips	1499:1520	riparian buffer strips	1499:1520	Based on the 16S rRNA gene copy number of the detected phylotype, the bacterial rhizospheres of plants in riparian buffer strips can be ranked as follows:Discocleidion rufescens > Phragmites australis > Vetiveria zizanioides > Pyrus betulifolia.
30628301	10	74	from	protection	1833:1842	arg1	strips					1863:1868	riparian buffer strips	1847:1868	riparian buffer strips of the Danjiangkou Reservoir	1847:1897	We analyzed the differences of different plants from the perspective of bacterial community composition and function and provide a foundation for vegetation construction and water environmental protection in riparian buffer strips of the Danjiangkou Reservoir.
30628301	4	75	theme	bacteria	677:684	arg1	diversity					664:672	diversity	664:672	diversity	664:672	High-throughput sequencing was performed to analyze the community structure and diversity of bacteria.
30628301	4	75	theme	bacteria	677:684	arg1	structure					650:658	community structure	640:658	community structure	640:658	High-throughput sequencing was performed to analyze the community structure and diversity of bacteria.
30628301	0	76	theme	Buffer	97:102	arg1	Strips					104:109	Riparian Buffer Strips	88:109	Riparian Buffer Strips in the Danjiangkou Reservoir, China]	88:146	[Composition and Predictive Functional Analysis of Rhizosphere Bacterial Communities in Riparian Buffer Strips in the Danjiangkou Reservoir, China].
30628301	7	77	theme	bacterial	1117:1125	arg1	communities					1127:1137	the observed bacterial communities	1104:1137	the observed bacterial communities	1104:1137	Phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) was used to determine the metabolic and functional abilities of the observed bacterial communities.
30628301	7	78	theme	states	1023:1028	arg1	reconstruction					994:1007	reconstruction	994:1007	reconstruction of unobserved states (PICRUSt)	994:1038	Phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) was used to determine the metabolic and functional abilities of the observed bacterial communities.
30628301	3	79	dep	trees	477:481	arg1	betulifolia					490:500	Pyrus betulifolia	484:500	Pyrus betulifolia	484:500	In this study, rhizosphere soil samples were collected from herbs (Vetiveria zizanioides and Phragmites australis), trees (Pyrus betulifolia), and shrubs (Discocleidion rufescens) in the Danjiangkou Reservoir in June 2017.
30628301	0	80	theme	Danjiangkou	118:128	arg1	China					141:145	the Danjiangkou Reservoir, China]	114:146	China	141:145	[Composition and Predictive Functional Analysis of Rhizosphere Bacterial Communities in Riparian Buffer Strips in the Danjiangkou Reservoir, China].
30628301	5	81	theme	phylogenetic	803:814	arg1	groups					816:821	31 major phylogenetic groups	794:821	31 major phylogenetic groups	794:821	Phylogenetic analysis based on 16S rDNA sequences shows that the bacterial communities can be divided into 31 major phylogenetic groups.
30628301	3	82	dep	herbs	421:425	arg1	zizanioides					438:448	zizanioides	438:448	zizanioides	438:448	In this study, rhizosphere soil samples were collected from herbs (Vetiveria zizanioides and Phragmites australis), trees (Pyrus betulifolia), and shrubs (Discocleidion rufescens) in the Danjiangkou Reservoir in June 2017.
30628301	3	82	dep	herbs	421:425	arg1	Phragmites					454:463	Phragmites	454:463	Phragmites	454:463	In this study, rhizosphere soil samples were collected from herbs (Vetiveria zizanioides and Phragmites australis), trees (Pyrus betulifolia), and shrubs (Discocleidion rufescens) in the Danjiangkou Reservoir in June 2017.
30628301	3	82	dep	herbs	421:425	arg1	herbs					421:425	herbs	421:425	herbs (Vetiveria zizanioides and Phragmites australis)	421:474	In this study, rhizosphere soil samples were collected from herbs (Vetiveria zizanioides and Phragmites australis), trees (Pyrus betulifolia), and shrubs (Discocleidion rufescens) in the Danjiangkou Reservoir in June 2017.
30628301	9	83	dep	Phragmites	1573:1582	arg1	australis					1584:1592	> Phragmites australis	1571:1592	follows:Discocleidion rufescens > Phragmites australis > Vetiveria zizanioides > Pyrus betulifolia	1539:1636	Based on the 16S rRNA gene copy number of the detected phylotype, the bacterial rhizospheres of plants in riparian buffer strips can be ranked as follows:Discocleidion rufescens > Phragmites australis > Vetiveria zizanioides > Pyrus betulifolia.
30628301	0	84	theme	Functional	28:37	arg1	Analysis					39:46	Predictive Functional Analysis	17:46	Predictive Functional Analysis	17:46	[Composition and Predictive Functional Analysis of Rhizosphere Bacterial Communities in Riparian Buffer Strips in the Danjiangkou Reservoir, China].
30628301	3	85	theme	rhizosphere	376:386	arg1	samples					393:399	rhizosphere soil samples	376:399	rhizosphere soil samples	376:399	In this study, rhizosphere soil samples were collected from herbs (Vetiveria zizanioides and Phragmites australis), trees (Pyrus betulifolia), and shrubs (Discocleidion rufescens) in the Danjiangkou Reservoir in June 2017.
30628301	9	86	theme	16S	1406:1408	arg1	rRNA					1410:1413	16S rRNA	1406:1413	the 16S rRNA gene copy number of the detected phylotype	1402:1456	Based on the 16S rRNA gene copy number of the detected phylotype, the bacterial rhizospheres of plants in riparian buffer strips can be ranked as follows:Discocleidion rufescens > Phragmites australis > Vetiveria zizanioides > Pyrus betulifolia.
30628301	8	87	theme	cell	1341:1344	arg1	growth					1346:1351	cell growth	1341:1351	cell growth	1341:1351	Our results reveal a wide genetic diversity of organisms involved in various essential processes such as biosynthesis of other secondary metabolites, transcription, Glycan biosynthesis and metabolism, cell growth and death, and carbohydrate metabolism.
30628301	2	88	from	compositions	257:268	arg1	strips					330:335	riparian buffer strips	314:335	riparian buffer strips	314:335	To our knowledge, the compositions and functions of rhizosphere communities in riparian buffer strips have not been reported.
30628301	10	89	theme	community	1721:1729	arg1	composition					1731:1741	bacterial community composition	1711:1741	bacterial community composition	1711:1741	We analyzed the differences of different plants from the perspective of bacterial community composition and function and provide a foundation for vegetation construction and water environmental protection in riparian buffer strips of the Danjiangkou Reservoir.
30628301	0	90	theme	Bacterial	63:71	arg1	Communities					73:83	Rhizosphere Bacterial Communities	51:83	Rhizosphere Bacterial Communities	51:83	[Composition and Predictive Functional Analysis of Rhizosphere Bacterial Communities in Riparian Buffer Strips in the Danjiangkou Reservoir, China].
30628301	9	91	theme	gene	1415:1418	arg1	number					1425:1430	the 16S rRNA gene copy number	1402:1430	the 16S rRNA gene copy number of the detected phylotype	1402:1456	Based on the 16S rRNA gene copy number of the detected phylotype, the bacterial rhizospheres of plants in riparian buffer strips can be ranked as follows:Discocleidion rufescens > Phragmites australis > Vetiveria zizanioides > Pyrus betulifolia.
30628301	8	92	theme	genetic	1166:1172	arg1	diversity					1174:1182	a wide genetic diversity	1159:1182	a wide genetic diversity of organisms involved in various essential processes such as biosynthesis of other secondary metabolites, transcription, Glycan biosynthesis and metabolism, cell growth and death, and carbohydrate metabolism	1159:1390	Our results reveal a wide genetic diversity of organisms involved in various essential processes such as biosynthesis of other secondary metabolites, transcription, Glycan biosynthesis and metabolism, cell growth and death, and carbohydrate metabolism.
30628301	3	93	dep	Phragmites	454:463	arg1	australis					465:473	Phragmites australis	454:473	Phragmites australis	454:473	In this study, rhizosphere soil samples were collected from herbs (Vetiveria zizanioides and Phragmites australis), trees (Pyrus betulifolia), and shrubs (Discocleidion rufescens) in the Danjiangkou Reservoir in June 2017.
30628301	6	94	theme	dominant	828:835	arg1	groups					850:855	The dominant phylogenetic groups	824:855	The dominant phylogenetic groups	824:855	The dominant phylogenetic groups include Proteobacteria, Bacteroidetes, Actinobacteria, Gemmatimonadetes, and Acidobacteria.
30628301	9	95	theme	phylotype	1448:1456	arg1	number					1425:1430	the 16S rRNA gene copy number	1402:1430	the 16S rRNA gene copy number of the detected phylotype	1402:1456	Based on the 16S rRNA gene copy number of the detected phylotype, the bacterial rhizospheres of plants in riparian buffer strips can be ranked as follows:Discocleidion rufescens > Phragmites australis > Vetiveria zizanioides > Pyrus betulifolia.
29334221	2	0	used	utilized	292:299	arg2	we					289:290	we	289:290	we	289:290	In this study, we utilized integrated functional omics technology to analyze composition and functional characteristics of extracellular enzymes produced by Aspergillus niger grown on food processing byproducts.
29334221	0	1	theme	Processing	107:116	arg1	Byproducts					118:127	Food Processing Byproducts	102:127	Food Processing Byproducts	102:127	Extracellular Enzyme Composition and Functional Characteristics of Aspergillus niger An-76 Induced by Food Processing Byproducts and Based on Integrated Functional Omics.
29334221	5	2	theme	peak	1026:1029	arg1	activity					1038:1045	peak enzyme activity	1026:1045	peak enzyme activity	1026:1045	Compared with proteins induced by single wheat bran or soybean dregs, the protein yield induced by their mixture was doubled, and the time required to reach peak enzyme activity was shortened by 25%.
29334221	2	3	theme	integrated	301:310	arg1	technology					329:338	integrated functional omics technology	301:338	integrated functional omics technology	301:338	In this study, we utilized integrated functional omics technology to analyze composition and functional characteristics of extracellular enzymes produced by Aspergillus niger grown on food processing byproducts.
29334221	4	4	theme	wheat	704:708	arg1	bran					710:713	wheat bran	704:713	wheat bran	704:713	Compared with other substrates, wheat bran was more effective at inducing the secretion of β-glucosidases from GH1 and GH3 families, as well as >50% of proteases from A1-family aspartic proteases.
29334221	0	5	theme	Food	102:105	arg1	Byproducts					118:127	Food Processing Byproducts	102:127	Food Processing Byproducts	102:127	Extracellular Enzyme Composition and Functional Characteristics of Aspergillus niger An-76 Induced by Food Processing Byproducts and Based on Integrated Functional Omics.
29334221	6	6	theme	analysis	1175:1182	arg1	formulation					1126:1136	the complex formulation	1114:1136	the complex formulation of various substrates and functional analysis of extracellular enzymes	1114:1207	This study provided a technical platform for the complex formulation of various substrates and functional analysis of extracellular enzymes.
29334221	6	7	theme	complex	1118:1124	arg1	formulation					1126:1136	the complex formulation	1114:1136	the complex formulation of various substrates and functional analysis of extracellular enzymes	1114:1207	This study provided a technical platform for the complex formulation of various substrates and functional analysis of extracellular enzymes.
29334221	4	8	from	families	795:802	arg1	secretion					750:758	the secretion	746:758	the secretion of β-glucosidases from GH1 and GH3 families	746:802	Compared with other substrates, wheat bran was more effective at inducing the secretion of β-glucosidases from GH1 and GH3 families, as well as >50% of proteases from A1-family aspartic proteases.
29334221	3	9	theme	wheat	576:580	arg1	bran					582:585	wheat bran	576:585	wheat bran	576:585	The results showed that oligosaccharides constituted by arabinose, xylose, and glucose in wheat bran were able to efficiently induce the production of extracellular enzymes of A. niger.
29334221	1	10	theme	food	185:188	arg1	processing					190:199	food processing	185:199	food processing	185:199	Byproducts of food processing can be utilized for the production of high-value-added enzyme cocktails.
29334221	1	11	theme	processing	190:199	arg1	Byproducts					171:180	Byproducts	171:180	Byproducts of food processing	171:199	Byproducts of food processing can be utilized for the production of high-value-added enzyme cocktails.
29334221	0	12	theme	Enzyme	14:19	arg1	Composition					21:31	Extracellular Enzyme Composition	0:31	Extracellular Enzyme Composition	0:31	Extracellular Enzyme Composition and Functional Characteristics of Aspergillus niger An-76 Induced by Food Processing Byproducts and Based on Integrated Functional Omics.
29334221	4	13	from	proteases	858:866	arg1	%					819:819	>50%	816:819	>50% of proteases from A1-family aspartic proteases	816:866	Compared with other substrates, wheat bran was more effective at inducing the secretion of β-glucosidases from GH1 and GH3 families, as well as >50% of proteases from A1-family aspartic proteases.
29334221	4	13	from	proteases	858:866	arg1	proteases					824:832	proteases	824:832	proteases from A1-family aspartic proteases	824:866	Compared with other substrates, wheat bran was more effective at inducing the secretion of β-glucosidases from GH1 and GH3 families, as well as >50% of proteases from A1-family aspartic proteases.
29334221	6	14	theme	substrates	1149:1158	arg1	formulation					1126:1136	the complex formulation	1114:1136	the complex formulation of various substrates and functional analysis of extracellular enzymes	1114:1207	This study provided a technical platform for the complex formulation of various substrates and functional analysis of extracellular enzymes.
29334221	0	15	theme	Extracellular	0:12	arg1	Composition					21:31	Extracellular Enzyme Composition	0:31	Extracellular Enzyme Composition	0:31	Extracellular Enzyme Composition and Functional Characteristics of Aspergillus niger An-76 Induced by Food Processing Byproducts and Based on Integrated Functional Omics.
29334221	4	16	from	GH1	783:785	arg1	secretion					750:758	the secretion	746:758	the secretion of β-glucosidases from GH1 and GH3 families	746:802	Compared with other substrates, wheat bran was more effective at inducing the secretion of β-glucosidases from GH1 and GH3 families, as well as >50% of proteases from A1-family aspartic proteases.
29334221	6	17	theme	various	1141:1147	arg1	substrates					1149:1158	various substrates	1141:1158	various substrates	1141:1158	This study provided a technical platform for the complex formulation of various substrates and functional analysis of extracellular enzymes.
29334221	5	18	theme	single	903:908	arg1	bran					916:919	single wheat bran	903:919	single wheat bran	903:919	Compared with proteins induced by single wheat bran or soybean dregs, the protein yield induced by their mixture was doubled, and the time required to reach peak enzyme activity was shortened by 25%.
29334221	5	19	dep	bran	916:919	arg1	dregs					932:936	dregs	932:936	dregs	932:936	Compared with proteins induced by single wheat bran or soybean dregs, the protein yield induced by their mixture was doubled, and the time required to reach peak enzyme activity was shortened by 25%.
29334221	4	20	theme	A1-family	839:847	arg1	proteases					858:866	A1-family aspartic proteases	839:866	A1-family aspartic proteases	839:866	Compared with other substrates, wheat bran was more effective at inducing the secretion of β-glucosidases from GH1 and GH3 families, as well as >50% of proteases from A1-family aspartic proteases.
29334221	2	21	theme	omics	323:327	arg1	technology					329:338	integrated functional omics technology	301:338	integrated functional omics technology	301:338	In this study, we utilized integrated functional omics technology to analyze composition and functional characteristics of extracellular enzymes produced by Aspergillus niger grown on food processing byproducts.
29334221	2	22	theme	processing	463:472	arg1	byproducts					474:483	food processing byproducts	458:483	food processing byproducts	458:483	In this study, we utilized integrated functional omics technology to analyze composition and functional characteristics of extracellular enzymes produced by Aspergillus niger grown on food processing byproducts.
29334221	6	23	theme	functional	1164:1173	arg1	analysis					1175:1182	functional analysis	1164:1182	functional analysis of extracellular enzymes	1164:1207	This study provided a technical platform for the complex formulation of various substrates and functional analysis of extracellular enzymes.
29334221	2	24	theme	functional	312:321	arg1	technology					329:338	integrated functional omics technology	301:338	integrated functional omics technology	301:338	In this study, we utilized integrated functional omics technology to analyze composition and functional characteristics of extracellular enzymes produced by Aspergillus niger grown on food processing byproducts.
29334221	2	25	theme	food	458:461	arg1	byproducts					474:483	food processing byproducts	458:483	food processing byproducts	458:483	In this study, we utilized integrated functional omics technology to analyze composition and functional characteristics of extracellular enzymes produced by Aspergillus niger grown on food processing byproducts.
29334221	0	26	theme	Functional	153:162	arg1	Omics					164:168	Integrated Functional Omics	142:168	Integrated Functional Omics	142:168	Extracellular Enzyme Composition and Functional Characteristics of Aspergillus niger An-76 Induced by Food Processing Byproducts and Based on Integrated Functional Omics.
29334221	3	27	theme	extracellular	637:649	arg1	enzymes					651:657	extracellular enzymes	637:657	extracellular enzymes of A. niger	637:669	The results showed that oligosaccharides constituted by arabinose, xylose, and glucose in wheat bran were able to efficiently induce the production of extracellular enzymes of A. niger.
29334221	0	28	theme	Functional	37:46	arg1	Characteristics					48:62	Functional Characteristics	37:62	Functional Characteristics	37:62	Extracellular Enzyme Composition and Functional Characteristics of Aspergillus niger An-76 Induced by Food Processing Byproducts and Based on Integrated Functional Omics.
29334221	0	29	theme	Integrated	142:151	arg1	Omics					164:168	Integrated Functional Omics	142:168	Integrated Functional Omics	142:168	Extracellular Enzyme Composition and Functional Characteristics of Aspergillus niger An-76 Induced by Food Processing Byproducts and Based on Integrated Functional Omics.
29334221	3	30	theme	enzymes	651:657	arg1	production					623:632	the production	619:632	the production of extracellular enzymes of A. niger	619:669	The results showed that oligosaccharides constituted by arabinose, xylose, and glucose in wheat bran were able to efficiently induce the production of extracellular enzymes of A. niger.
29334221	4	31	theme	other	686:690	arg1	substrates					692:701	other substrates	686:701	other substrates	686:701	Compared with other substrates, wheat bran was more effective at inducing the secretion of β-glucosidases from GH1 and GH3 families, as well as >50% of proteases from A1-family aspartic proteases.
29334221	0	32	theme	Aspergillus	67:77	arg1	niger					79:83	Aspergillus niger An-76	67:89	Aspergillus niger An-76	67:89	Extracellular Enzyme Composition and Functional Characteristics of Aspergillus niger An-76 Induced by Food Processing Byproducts and Based on Integrated Functional Omics.
29334221	5	33	theme	wheat	910:914	arg1	bran					916:919	single wheat bran	903:919	single wheat bran	903:919	Compared with proteins induced by single wheat bran or soybean dregs, the protein yield induced by their mixture was doubled, and the time required to reach peak enzyme activity was shortened by 25%.
29334221	4	34	theme	aspartic	849:856	arg1	proteases					858:866	A1-family aspartic proteases	839:866	A1-family aspartic proteases	839:866	Compared with other substrates, wheat bran was more effective at inducing the secretion of β-glucosidases from GH1 and GH3 families, as well as >50% of proteases from A1-family aspartic proteases.
29334221	6	35	theme	technical	1091:1099	arg1	platform					1101:1108	a technical platform	1089:1108	a technical platform for the complex formulation of various substrates and functional analysis of extracellular enzymes	1089:1207	This study provided a technical platform for the complex formulation of various substrates and functional analysis of extracellular enzymes.
29334221	2	36	theme	functional	367:376	arg1	characteristics					378:392	functional characteristics	367:392	functional characteristics	367:392	In this study, we utilized integrated functional omics technology to analyze composition and functional characteristics of extracellular enzymes produced by Aspergillus niger grown on food processing byproducts.
29334221	5	37	theme	enzyme	1031:1036	arg1	activity					1038:1045	peak enzyme activity	1026:1045	peak enzyme activity	1026:1045	Compared with proteins induced by single wheat bran or soybean dregs, the protein yield induced by their mixture was doubled, and the time required to reach peak enzyme activity was shortened by 25%.
29334221	1	38	used	utilized	208:215	arg2	Byproducts					171:180	Byproducts	171:180	Byproducts of food processing	171:199	Byproducts of food processing can be utilized for the production of high-value-added enzyme cocktails.
29334221	3	39	theme	niger	665:669	arg1	enzymes					651:657	extracellular enzymes	637:657	extracellular enzymes of A. niger	637:669	The results showed that oligosaccharides constituted by arabinose, xylose, and glucose in wheat bran were able to efficiently induce the production of extracellular enzymes of A. niger.
29334221	4	40	theme	proteases	824:832	arg1	%					819:819	>50%	816:819	>50% of proteases from A1-family aspartic proteases	816:866	Compared with other substrates, wheat bran was more effective at inducing the secretion of β-glucosidases from GH1 and GH3 families, as well as >50% of proteases from A1-family aspartic proteases.
29334221	4	40	theme	proteases	824:832	arg1	proteases					824:832	proteases	824:832	proteases from A1-family aspartic proteases	824:866	Compared with other substrates, wheat bran was more effective at inducing the secretion of β-glucosidases from GH1 and GH3 families, as well as >50% of proteases from A1-family aspartic proteases.
29334221	4	41	theme	β-glucosidases	763:776	arg1	secretion					750:758	the secretion	746:758	the secretion of β-glucosidases from GH1 and GH3 families	746:802	Compared with other substrates, wheat bran was more effective at inducing the secretion of β-glucosidases from GH1 and GH3 families, as well as >50% of proteases from A1-family aspartic proteases.
29334221	4	42	theme	GH3	791:793	arg1	families					795:802	GH3 families	791:802	GH3 families	791:802	Compared with other substrates, wheat bran was more effective at inducing the secretion of β-glucosidases from GH1 and GH3 families, as well as >50% of proteases from A1-family aspartic proteases.
29334221	5	43	theme	protein	943:949	arg1	yield					951:955	the protein yield	939:955	the protein yield induced by their mixture	939:980	Compared with proteins induced by single wheat bran or soybean dregs, the protein yield induced by their mixture was doubled, and the time required to reach peak enzyme activity was shortened by 25%.
29334221	1	44	theme	high-value-added	239:254	arg1	cocktails					263:271	high-value-added enzyme cocktails	239:271	high-value-added enzyme cocktails	239:271	Byproducts of food processing can be utilized for the production of high-value-added enzyme cocktails.
29334221	6	45	theme	enzymes	1201:1207	arg1	substrates					1149:1158	various substrates	1141:1158	various substrates	1141:1158	This study provided a technical platform for the complex formulation of various substrates and functional analysis of extracellular enzymes.
29334221	6	45	theme	enzymes	1201:1207	arg1	analysis					1175:1182	functional analysis	1164:1182	functional analysis of extracellular enzymes	1164:1207	This study provided a technical platform for the complex formulation of various substrates and functional analysis of extracellular enzymes.
29334221	4	46	from	%	819:819	arg1	proteases					858:866	A1-family aspartic proteases	839:866	A1-family aspartic proteases	839:866	Compared with other substrates, wheat bran was more effective at inducing the secretion of β-glucosidases from GH1 and GH3 families, as well as >50% of proteases from A1-family aspartic proteases.
29334221	2	47	theme	enzymes	411:417	arg1	composition					351:361	composition	351:361	composition	351:361	In this study, we utilized integrated functional omics technology to analyze composition and functional characteristics of extracellular enzymes produced by Aspergillus niger grown on food processing byproducts.
29334221	2	47	theme	enzymes	411:417	arg1	characteristics					378:392	functional characteristics	367:392	functional characteristics	367:392	In this study, we utilized integrated functional omics technology to analyze composition and functional characteristics of extracellular enzymes produced by Aspergillus niger grown on food processing byproducts.
29334221	2	48	theme	Aspergillus	431:441	arg1	niger					443:447	Aspergillus niger	431:447	Aspergillus niger grown on food processing byproducts	431:483	In this study, we utilized integrated functional omics technology to analyze composition and functional characteristics of extracellular enzymes produced by Aspergillus niger grown on food processing byproducts.
29334221	1	49	theme	enzyme	256:261	arg1	cocktails					263:271	high-value-added enzyme cocktails	239:271	high-value-added enzyme cocktails	239:271	Byproducts of food processing can be utilized for the production of high-value-added enzyme cocktails.
29334221	0	50	theme	niger	79:83	arg1	Composition					21:31	Extracellular Enzyme Composition	0:31	Extracellular Enzyme Composition	0:31	Extracellular Enzyme Composition and Functional Characteristics of Aspergillus niger An-76 Induced by Food Processing Byproducts and Based on Integrated Functional Omics.
29334221	0	50	theme	niger	79:83	arg1	Characteristics					48:62	Functional Characteristics	37:62	Functional Characteristics	37:62	Extracellular Enzyme Composition and Functional Characteristics of Aspergillus niger An-76 Induced by Food Processing Byproducts and Based on Integrated Functional Omics.
29334221	6	51	theme	extracellular	1187:1199	arg1	enzymes					1201:1207	extracellular enzymes	1187:1207	extracellular enzymes	1187:1207	This study provided a technical platform for the complex formulation of various substrates and functional analysis of extracellular enzymes.
29334221	2	52	theme	extracellular	397:409	arg1	enzymes					411:417	extracellular enzymes	397:417	extracellular enzymes produced by Aspergillus niger grown on food processing byproducts	397:483	In this study, we utilized integrated functional omics technology to analyze composition and functional characteristics of extracellular enzymes produced by Aspergillus niger grown on food processing byproducts.
29334221	1	53	theme	cocktails	263:271	arg1	production					225:234	the production	221:234	the production of high-value-added enzyme cocktails	221:271	Byproducts of food processing can be utilized for the production of high-value-added enzyme cocktails.
31746260	0	0	theme	in vitro	76:83	arg1	digestibility					92:104	in vitro starch digestibility	76:104	in vitro starch digestibility with no major effects on glycaemic responses	76:149	Microstructure of starch-based meals with either palm or soybean oils alter in vitro starch digestibility with no major effects on glycaemic responses.
31746260	6	1	theme	similar	978:984	arg1	responses					1018:1026	very similar postprandial glucose or insulin responses	973:1026	very similar postprandial glucose or insulin responses	973:1026	Administration of starch/gluten-based meals with either palm or soybean oils to volunteers show very similar postprandial glucose or insulin responses.
31746260	4	2	theme	gelatinisation	687:700	arg1	temperature					702:712	similar starch gelatinisation temperature	672:712	similar starch gelatinisation temperature	672:712	Both types of foods generated similar starch gelatinisation temperature.
31746260	1	3	theme	Glycaemic	152:160	arg1	GR					172:173	GR	172:173	GR	172:173	Glycaemic response (GR) to starch-based meals depends on their food composition and microstructure.
31746260	1	3	theme	Glycaemic	152:160	arg1	response					162:169	Glycaemic response	152:169	Glycaemic response (GR) to starch-based meals	152:196	Glycaemic response (GR) to starch-based meals depends on their food composition and microstructure.
31746260	6	4	dep	palm	933:936	arg1	oils					949:952	oils	949:952	oils	949:952	Administration of starch/gluten-based meals with either palm or soybean oils to volunteers show very similar postprandial glucose or insulin responses.
31746260	6	5	with	meals	915:919	arg1	palm					933:936	palm	933:936	palm	933:936	Administration of starch/gluten-based meals with either palm or soybean oils to volunteers show very similar postprandial glucose or insulin responses.
31746260	6	5	with	meals	915:919	arg1	soybean					941:947	soybean	941:947	soybean	941:947	Administration of starch/gluten-based meals with either palm or soybean oils to volunteers show very similar postprandial glucose or insulin responses.
31746260	2	6	theme	solid	326:330	arg1	matrix					350:355	a solid starch-oil-gluten matrix	324:355	a solid starch-oil-gluten matrix	324:355	We studied the effect of palm and soybean oils on the microstructure of a solid starch-oil-gluten matrix, on the starch gelatinisation and in vitro digestibility.
31746260	7	7	theme	major	1153:1157	arg1	effects					1159:1165	no major effects	1150:1165	no major effects on glycaemic responses in female volunteers	1150:1209	In conclusion, differences in fatty acid composition changes food microstructure and in vitro starch digestibility, with no major effects on glycaemic responses in female volunteers.
31746260	3	8	theme	soybean	570:576	arg1	oil					578:580	soybean oil	570:580	soybean oil	570:580	Additionally, a pilot cross-over study was carried out to assess GR after eating gelatinised starch/gluten-based foods with the addition of either palm or soybean oil in 8 young non-diabetic female volunteers (ISRCTN39636850).
31746260	2	9	theme	palm	277:280	arg1	oils					294:297	palm and soybean oils	277:297	palm and soybean oils	277:297	We studied the effect of palm and soybean oils on the microstructure of a solid starch-oil-gluten matrix, on the starch gelatinisation and in vitro digestibility.
31746260	6	10	theme	insulin	1010:1016	arg1	responses					1018:1026	very similar postprandial glucose or insulin responses	973:1026	very similar postprandial glucose or insulin responses	973:1026	Administration of starch/gluten-based meals with either palm or soybean oils to volunteers show very similar postprandial glucose or insulin responses.
31746260	0	11	theme	starch	85:90	arg1	digestibility					92:104	in vitro starch digestibility	76:104	in vitro starch digestibility with no major effects on glycaemic responses	76:149	Microstructure of starch-based meals with either palm or soybean oils alter in vitro starch digestibility with no major effects on glycaemic responses.
31746260	5	12	contain	had	757:759	arg2	microstructure					769:782	rougher microstructure	761:782	rougher microstructure	761:782	Starch/gluten-based food with soybean oil had rougher microstructure compared to food with palm oil, showing a higher initial and lower final in vitro digestion.
31746260	5	12	contain	had	757:759	arg1	food					735:738	Starch/gluten-based food	715:738	Starch/gluten-based food with soybean oil	715:755	Starch/gluten-based food with soybean oil had rougher microstructure compared to food with palm oil, showing a higher initial and lower final in vitro digestion.
31746260	2	13	theme	starch-oil-gluten	332:348	arg1	matrix					350:355	a solid starch-oil-gluten matrix	324:355	a solid starch-oil-gluten matrix	324:355	We studied the effect of palm and soybean oils on the microstructure of a solid starch-oil-gluten matrix, on the starch gelatinisation and in vitro digestibility.
31746260	6	14	theme	meals	915:919	arg1	Administration					877:890	Administration	877:890	Administration of starch/gluten-based meals with either palm or soybean oils to volunteers	877:966	Administration of starch/gluten-based meals with either palm or soybean oils to volunteers show very similar postprandial glucose or insulin responses.
31746260	5	15	with	food	735:738	arg1	oil					753:755	soybean oil	745:755	soybean oil	745:755	Starch/gluten-based food with soybean oil had rougher microstructure compared to food with palm oil, showing a higher initial and lower final in vitro digestion.
31746260	5	16	theme	in vitro	857:864	arg1	digestion					866:874	a higher initial and lower final in vitro digestion	824:874	a higher initial and lower final in vitro digestion	824:874	Starch/gluten-based food with soybean oil had rougher microstructure compared to food with palm oil, showing a higher initial and lower final in vitro digestion.
31746260	6	17	theme	starch/gluten-based	895:913	arg1	meals					915:919	starch/gluten-based meals	895:919	starch/gluten-based meals with either palm or soybean oils	895:952	Administration of starch/gluten-based meals with either palm or soybean oils to volunteers show very similar postprandial glucose or insulin responses.
31746260	2	18	theme	starch	365:370	arg1	gelatinisation					372:385	starch gelatinisation	365:385	starch gelatinisation	365:385	We studied the effect of palm and soybean oils on the microstructure of a solid starch-oil-gluten matrix, on the starch gelatinisation and in vitro digestibility.
31746260	7	19	theme	in vitro	1114:1121	arg1	digestibility					1130:1142	in vitro starch digestibility	1114:1142	in vitro starch digestibility	1114:1142	In conclusion, differences in fatty acid composition changes food microstructure and in vitro starch digestibility, with no major effects on glycaemic responses in female volunteers.
31746260	3	20	theme	palm	562:565	arg1	addition					543:550	the addition	539:550	the addition of either palm or soybean oil	539:580	Additionally, a pilot cross-over study was carried out to assess GR after eating gelatinised starch/gluten-based foods with the addition of either palm or soybean oil in 8 young non-diabetic female volunteers (ISRCTN39636850).
31746260	4	21	theme	foods	656:660	arg1	types					647:651	Both types	642:651	Both types of foods	642:660	Both types of foods generated similar starch gelatinisation temperature.
31746260	5	22	theme	Starch/gluten-based	715:733	arg1	food					735:738	Starch/gluten-based food	715:738	Starch/gluten-based food with soybean oil	715:755	Starch/gluten-based food with soybean oil had rougher microstructure compared to food with palm oil, showing a higher initial and lower final in vitro digestion.
31746260	6	23	theme	glucose	999:1005	arg1	responses					1018:1026	very similar postprandial glucose or insulin responses	973:1026	very similar postprandial glucose or insulin responses	973:1026	Administration of starch/gluten-based meals with either palm or soybean oils to volunteers show very similar postprandial glucose or insulin responses.
31746260	7	24	theme	starch	1123:1128	arg1	digestibility					1130:1142	in vitro starch digestibility	1114:1142	in vitro starch digestibility	1114:1142	In conclusion, differences in fatty acid composition changes food microstructure and in vitro starch digestibility, with no major effects on glycaemic responses in female volunteers.
31746260	0	25	with	digestibility	92:104	arg1	effects					120:126	no major effects	111:126	no major effects on glycaemic responses	111:149	Microstructure of starch-based meals with either palm or soybean oils alter in vitro starch digestibility with no major effects on glycaemic responses.
31746260	2	26	theme	oils	294:297	arg1	effect					267:272	the effect	263:272	the effect of palm and soybean oils on the microstructure of a solid starch-oil-gluten matrix	263:355	We studied the effect of palm and soybean oils on the microstructure of a solid starch-oil-gluten matrix, on the starch gelatinisation and in vitro digestibility.
31746260	0	27	theme	meals	31:35	arg1	Microstructure					0:13	Microstructure	0:13	Microstructure of starch-based meals with either palm or soybean oils	0:68	Microstructure of starch-based meals with either palm or soybean oils alter in vitro starch digestibility with no major effects on glycaemic responses.
31746260	3	28	theme	young	587:591	arg1	ISRCTN39636850					625:638	ISRCTN39636850	625:638	ISRCTN39636850	625:638	Additionally, a pilot cross-over study was carried out to assess GR after eating gelatinised starch/gluten-based foods with the addition of either palm or soybean oil in 8 young non-diabetic female volunteers (ISRCTN39636850).
31746260	3	28	theme	young	587:591	arg1	volunteers					613:622	8 young non-diabetic female volunteers	585:622	8 young non-diabetic female volunteers (ISRCTN39636850)	585:639	Additionally, a pilot cross-over study was carried out to assess GR after eating gelatinised starch/gluten-based foods with the addition of either palm or soybean oil in 8 young non-diabetic female volunteers (ISRCTN39636850).
31746260	2	29	theme	soybean	286:292	arg1	oils					294:297	palm and soybean oils	277:297	palm and soybean oils	277:297	We studied the effect of palm and soybean oils on the microstructure of a solid starch-oil-gluten matrix, on the starch gelatinisation and in vitro digestibility.
31746260	7	30	from	responses	1180:1188	arg1	volunteers					1200:1209	female volunteers	1193:1209	female volunteers	1193:1209	In conclusion, differences in fatty acid composition changes food microstructure and in vitro starch digestibility, with no major effects on glycaemic responses in female volunteers.
31746260	1	31	theme	starch-based	179:190	arg1	meals					192:196	starch-based meals	179:196	starch-based meals	179:196	Glycaemic response (GR) to starch-based meals depends on their food composition and microstructure.
31746260	0	32	theme	starch-based	18:29	arg1	meals					31:35	starch-based meals	18:35	starch-based meals	18:35	Microstructure of starch-based meals with either palm or soybean oils alter in vitro starch digestibility with no major effects on glycaemic responses.
31746260	5	33	dep	initial	833:839	arg1	higher					826:831	higher	826:831	higher	826:831	Starch/gluten-based food with soybean oil had rougher microstructure compared to food with palm oil, showing a higher initial and lower final in vitro digestion.
31746260	0	34	theme	major	114:118	arg1	effects					120:126	no major effects	111:126	no major effects on glycaemic responses	111:149	Microstructure of starch-based meals with either palm or soybean oils alter in vitro starch digestibility with no major effects on glycaemic responses.
31746260	4	35	theme	starch	680:685	arg1	temperature					702:712	similar starch gelatinisation temperature	672:712	similar starch gelatinisation temperature	672:712	Both types of foods generated similar starch gelatinisation temperature.
31746260	4	36	theme	similar	672:678	arg1	temperature					702:712	similar starch gelatinisation temperature	672:712	similar starch gelatinisation temperature	672:712	Both types of foods generated similar starch gelatinisation temperature.
31746260	0	37	theme	glycaemic	131:139	arg1	responses					141:149	glycaemic responses	131:149	glycaemic responses	131:149	Microstructure of starch-based meals with either palm or soybean oils alter in vitro starch digestibility with no major effects on glycaemic responses.
31746260	3	38	theme	cross-over	437:446	arg1	study					448:452	a pilot cross-over study	429:452	a pilot cross-over study	429:452	Additionally, a pilot cross-over study was carried out to assess GR after eating gelatinised starch/gluten-based foods with the addition of either palm or soybean oil in 8 young non-diabetic female volunteers (ISRCTN39636850).
31746260	2	39	theme	in vitro	391:398	arg1	digestibility					400:412	in vitro digestibility	391:412	in vitro digestibility	391:412	We studied the effect of palm and soybean oils on the microstructure of a solid starch-oil-gluten matrix, on the starch gelatinisation and in vitro digestibility.
31746260	7	40	theme	food	1090:1093	arg1	microstructure					1095:1108	food microstructure	1090:1108	food microstructure	1090:1108	In conclusion, differences in fatty acid composition changes food microstructure and in vitro starch digestibility, with no major effects on glycaemic responses in female volunteers.
31746260	7	41	theme	female	1193:1198	arg1	volunteers					1200:1209	female volunteers	1193:1209	female volunteers	1193:1209	In conclusion, differences in fatty acid composition changes food microstructure and in vitro starch digestibility, with no major effects on glycaemic responses in female volunteers.
31746260	5	42	theme	final	851:855	arg1	digestion					866:874	a higher initial and lower final in vitro digestion	824:874	a higher initial and lower final in vitro digestion	824:874	Starch/gluten-based food with soybean oil had rougher microstructure compared to food with palm oil, showing a higher initial and lower final in vitro digestion.
31746260	5	43	theme	palm	806:809	arg1	oil					811:813	palm oil	806:813	palm oil	806:813	Starch/gluten-based food with soybean oil had rougher microstructure compared to food with palm oil, showing a higher initial and lower final in vitro digestion.
31746260	7	44	theme	acid	1065:1068	arg1	composition					1070:1080	fatty acid composition	1059:1080	fatty acid composition	1059:1080	In conclusion, differences in fatty acid composition changes food microstructure and in vitro starch digestibility, with no major effects on glycaemic responses in female volunteers.
31746260	6	45	theme	postprandial	986:997	arg1	glucose					999:1005	postprandial glucose	986:1005	postprandial glucose	986:1005	Administration of starch/gluten-based meals with either palm or soybean oils to volunteers show very similar postprandial glucose or insulin responses.
31746260	0	46	dep	either	42:47	arg1	palm					49:52	palm	49:52	palm	49:52	Microstructure of starch-based meals with either palm or soybean oils alter in vitro starch digestibility with no major effects on glycaemic responses.
31746260	7	47	theme	glycaemic	1170:1178	arg1	responses					1180:1188	glycaemic responses	1170:1188	glycaemic responses in female volunteers	1170:1209	In conclusion, differences in fatty acid composition changes food microstructure and in vitro starch digestibility, with no major effects on glycaemic responses in female volunteers.
31746260	3	48	theme	oil	578:580	arg1	addition					543:550	the addition	539:550	the addition of either palm or soybean oil	539:580	Additionally, a pilot cross-over study was carried out to assess GR after eating gelatinised starch/gluten-based foods with the addition of either palm or soybean oil in 8 young non-diabetic female volunteers (ISRCTN39636850).
31746260	2	49	from	effect	267:272	arg1	microstructure					306:319	the microstructure	302:319	the microstructure of a solid starch-oil-gluten matrix	302:355	We studied the effect of palm and soybean oils on the microstructure of a solid starch-oil-gluten matrix, on the starch gelatinisation and in vitro digestibility.
31746260	7	50	from	effects	1159:1165	arg1	responses					1180:1188	glycaemic responses	1170:1188	glycaemic responses in female volunteers	1170:1209	In conclusion, differences in fatty acid composition changes food microstructure and in vitro starch digestibility, with no major effects on glycaemic responses in female volunteers.
31746260	3	51	theme	female	606:611	arg1	ISRCTN39636850					625:638	ISRCTN39636850	625:638	ISRCTN39636850	625:638	Additionally, a pilot cross-over study was carried out to assess GR after eating gelatinised starch/gluten-based foods with the addition of either palm or soybean oil in 8 young non-diabetic female volunteers (ISRCTN39636850).
31746260	3	51	theme	female	606:611	arg1	volunteers					613:622	8 young non-diabetic female volunteers	585:622	8 young non-diabetic female volunteers (ISRCTN39636850)	585:639	Additionally, a pilot cross-over study was carried out to assess GR after eating gelatinised starch/gluten-based foods with the addition of either palm or soybean oil in 8 young non-diabetic female volunteers (ISRCTN39636850).
31746260	0	52	with	Microstructure	0:13	arg1	oils					65:68	either palm or soybean oils	42:68	either palm or soybean oils	42:68	Microstructure of starch-based meals with either palm or soybean oils alter in vitro starch digestibility with no major effects on glycaemic responses.
31746260	0	53	dep	oils	65:68	arg1	either					42:47	either	42:47	either	42:47	Microstructure of starch-based meals with either palm or soybean oils alter in vitro starch digestibility with no major effects on glycaemic responses.
31746260	0	53	dep	oils	65:68	arg1	soybean					57:63	soybean	57:63	soybean	57:63	Microstructure of starch-based meals with either palm or soybean oils alter in vitro starch digestibility with no major effects on glycaemic responses.
31746260	7	54	theme	fatty	1059:1063	arg1	composition					1070:1080	fatty acid composition	1059:1080	fatty acid composition	1059:1080	In conclusion, differences in fatty acid composition changes food microstructure and in vitro starch digestibility, with no major effects on glycaemic responses in female volunteers.
31746260	3	55	theme	starch/gluten-based	508:526	arg1	foods					528:532	gelatinised starch/gluten-based foods	496:532	gelatinised starch/gluten-based foods	496:532	Additionally, a pilot cross-over study was carried out to assess GR after eating gelatinised starch/gluten-based foods with the addition of either palm or soybean oil in 8 young non-diabetic female volunteers (ISRCTN39636850).
31746260	1	56	theme	food	215:218	arg1	composition					220:230	their food composition	209:230	their food composition	209:230	Glycaemic response (GR) to starch-based meals depends on their food composition and microstructure.
31746260	5	57	theme	rougher	761:767	arg1	microstructure					769:782	rougher microstructure	761:782	rougher microstructure	761:782	Starch/gluten-based food with soybean oil had rougher microstructure compared to food with palm oil, showing a higher initial and lower final in vitro digestion.
31746260	7	58	from	differences	1044:1054	arg1	composition					1070:1080	fatty acid composition	1059:1080	fatty acid composition	1059:1080	In conclusion, differences in fatty acid composition changes food microstructure and in vitro starch digestibility, with no major effects on glycaemic responses in female volunteers.
31746260	2	59	dep	gelatinisation	372:385	arg1	the					361:363	the	361:363	the	361:363	We studied the effect of palm and soybean oils on the microstructure of a solid starch-oil-gluten matrix, on the starch gelatinisation and in vitro digestibility.
31746260	5	60	theme	initial	833:839	arg1	digestion					866:874	a higher initial and lower final in vitro digestion	824:874	a higher initial and lower final in vitro digestion	824:874	Starch/gluten-based food with soybean oil had rougher microstructure compared to food with palm oil, showing a higher initial and lower final in vitro digestion.
31746260	3	61	theme	gelatinised	496:506	arg1	foods					528:532	gelatinised starch/gluten-based foods	496:532	gelatinised starch/gluten-based foods	496:532	Additionally, a pilot cross-over study was carried out to assess GR after eating gelatinised starch/gluten-based foods with the addition of either palm or soybean oil in 8 young non-diabetic female volunteers (ISRCTN39636850).
31746260	3	62	theme	pilot	431:435	arg1	study					448:452	a pilot cross-over study	429:452	a pilot cross-over study	429:452	Additionally, a pilot cross-over study was carried out to assess GR after eating gelatinised starch/gluten-based foods with the addition of either palm or soybean oil in 8 young non-diabetic female volunteers (ISRCTN39636850).
31746260	0	63	from	effects	120:126	arg1	responses					141:149	glycaemic responses	131:149	glycaemic responses	131:149	Microstructure of starch-based meals with either palm or soybean oils alter in vitro starch digestibility with no major effects on glycaemic responses.
31746260	3	64	theme	non-diabetic	593:604	arg1	ISRCTN39636850					625:638	ISRCTN39636850	625:638	ISRCTN39636850	625:638	Additionally, a pilot cross-over study was carried out to assess GR after eating gelatinised starch/gluten-based foods with the addition of either palm or soybean oil in 8 young non-diabetic female volunteers (ISRCTN39636850).
31746260	3	64	theme	non-diabetic	593:604	arg1	volunteers					613:622	8 young non-diabetic female volunteers	585:622	8 young non-diabetic female volunteers (ISRCTN39636850)	585:639	Additionally, a pilot cross-over study was carried out to assess GR after eating gelatinised starch/gluten-based foods with the addition of either palm or soybean oil in 8 young non-diabetic female volunteers (ISRCTN39636850).
31746260	2	65	theme	matrix	350:355	arg1	microstructure					306:319	the microstructure	302:319	the microstructure of a solid starch-oil-gluten matrix	302:355	We studied the effect of palm and soybean oils on the microstructure of a solid starch-oil-gluten matrix, on the starch gelatinisation and in vitro digestibility.
31746260	5	66	theme	soybean	745:751	arg1	oil					753:755	soybean oil	745:755	soybean oil	745:755	Starch/gluten-based food with soybean oil had rougher microstructure compared to food with palm oil, showing a higher initial and lower final in vitro digestion.
29618164	8	0	theme	loci	820:823	arg1	analysis					831:838	quantitative trait loci (QTL) analysis	801:838	quantitative trait loci (QTL) analysis for protein, oil, and sucrose	801:868	In addition to bin mapping, quantitative trait loci (QTL) analysis for protein, oil, and sucrose was performed using 3343 polymorphic SNPs (3K-SNP), derived from Illumina Infinium BeadChip sequencing platform.
29618164	11	1	theme	genomic	1407:1413	arg1	loci					1415:1418	soybean domestication-related genomic loci	1377:1418	soybean domestication-related genomic loci	1377:1418	A total of five, nine, and four QTLs were identified for protein, oil, and sucrose content, respectively, and some of the QTLs coincided with soybean domestication-related genomic loci.
29618164	5	2	theme	interspecific	424:436	arg1	population					438:447	an interspecific population	421:447	an interspecific population	421:447	In this study, an interspecific population was derived from a cross between G. max (Williams 82) and G. soja (PI 483460B).
29618164	7	3	from	events	687:692	arg1	RILs					697:700	RILs	697:700	RILs	697:700	Based on 91 342 single nucleotide polymorphisms (SNPs), recombination events in RILs were defined, and a high-resolution bin map was developed (4070 bins).
29618164	17	4	theme	QTL-based	1802:1810	arg1	resequencing					1841:1852	A QTL-based haplotype using whole-genome resequencing	1800:1852	A QTL-based haplotype using whole-genome resequencing of 106 diverse soybean lines	1800:1881	A QTL-based haplotype using whole-genome resequencing of 106 diverse soybean lines identified unique allelic variation in wild soybean that could be utilized to widen the genetic base in cultivated soybean.
29618164	7	5	dep	developed	750:758	arg1	bins					766:769	4070 bins	761:769	4070 bins	761:769	Based on 91 342 single nucleotide polymorphisms (SNPs), recombination events in RILs were defined, and a high-resolution bin map was developed (4070 bins).
29618164	7	6	theme	high-resolution	722:736	arg1	map					742:744	a high-resolution bin map	720:744	a high-resolution bin map	720:744	Based on 91 342 single nucleotide polymorphisms (SNPs), recombination events in RILs were defined, and a high-resolution bin map was developed (4070 bins).
29618164	1	7	theme	cultivated	168:177	arg1	Merr					196:199	The cultivated [Glycine max (L) Merr	164:199	The cultivated [Glycine max (L) Merr.	164:200	The cultivated [Glycine max (L) Merr.]
29618164	17	8	theme	using	1822:1826	arg1	resequencing					1841:1852	A QTL-based haplotype using whole-genome resequencing	1800:1852	A QTL-based haplotype using whole-genome resequencing of 106 diverse soybean lines	1800:1881	A QTL-based haplotype using whole-genome resequencing of 106 diverse soybean lines identified unique allelic variation in wild soybean that could be utilized to widen the genetic base in cultivated soybean.
29618164	15	9	theme	putative	1648:1655	arg1	genes					1657:1661	putative genes	1648:1661	putative genes involved in sugar transport	1648:1689	8 (qSuc_08) and harbours putative genes involved in sugar transport.
29618164	1	10	theme	max	188:190	arg1	Merr					196:199	The cultivated [Glycine max (L) Merr	164:199	The cultivated [Glycine max (L) Merr.	164:200	The cultivated [Glycine max (L) Merr.]
29618164	4	11	theme	cultivated	337:346	arg1	soybean					348:354	cultivated soybean	337:354	cultivated soybean	337:354	Compared to wild soybean, cultivated soybean contains low protein, high oil, and high sucrose.
29618164	10	12	dep	enhanced	1185:1192	arg1	50 Kb					1227:1231	50 Kb	1227:1231	50 Kb	1227:1231	Importantly, the bin map derived from next-generation sequencing technology enhanced mapping resolution (from 1325 to 50 Kb).
29618164	1	13	theme	L	193:193	arg1	Merr					196:199	The cultivated [Glycine max (L) Merr	164:199	The cultivated [Glycine max (L) Merr.	164:200	The cultivated [Glycine max (L) Merr.]
29618164	4	14	theme	high	392:395	arg1	sucrose					397:403	high sucrose	392:403	high sucrose	392:403	Compared to wild soybean, cultivated soybean contains low protein, high oil, and high sucrose.
29618164	0	15	theme	soybean	120:126	arg1	population					106:115	an interspecific mapping population	81:115	an interspecific mapping population of soybean	81:126	Dissecting genomic hotspots underlying seed protein, oil, and sucrose content in an interspecific mapping population of soybean using high-density linkage mapping.
29618164	3	16	theme	wide	268:271	arg1	variation					273:281	wide variation	268:281	wide variation in seed composition traits	268:308	soybean species comprise wide variation in seed composition traits.
29618164	17	17	from	base	1979:1982	arg1	soybean					1998:2004	cultivated soybean	1987:2004	cultivated soybean	1987:2004	A QTL-based haplotype using whole-genome resequencing of 106 diverse soybean lines identified unique allelic variation in wild soybean that could be utilized to widen the genetic base in cultivated soybean.
29618164	0	18	theme	high-density	134:145	arg1	mapping					155:161	high-density linkage mapping	134:161	high-density linkage mapping	134:161	Dissecting genomic hotspots underlying seed protein, oil, and sucrose content in an interspecific mapping population of soybean using high-density linkage mapping.
29618164	4	19	theme	high	378:381	arg1	oil					383:385	high oil	378:385	high oil	378:385	Compared to wild soybean, cultivated soybean contains low protein, high oil, and high sucrose.
29618164	9	20	theme	3K-SNP	1093:1098	arg1	markers					1100:1106	3K-SNP markers	1093:1106	3K-SNP markers	1093:1106	The QTL regions from both platforms were compared, and a significant concordance was observed between bin and 3K-SNP markers.
29618164	3	21	theme	composition	291:301	arg1	traits					303:308	seed composition traits	286:308	seed composition traits	286:308	soybean species comprise wide variation in seed composition traits.
29618164	2	22	theme	[Glycine	212:219	arg1	soja					221:224	wild [Glycine soja	207:224	wild [Glycine soja	207:224	and wild [Glycine soja Siebold & Zucc.]
29618164	14	23	theme	major	1589:1593	arg1	QTL					1595:1597	a novel and major QTL	1577:1597	a novel and major QTL	1577:1597	In terms of sucrose content, a novel and major QTL were identified on Chr.
29618164	6	24	theme	inbred	546:551	arg1	population					564:573	This recombinant inbred line (RIL) population	529:573	This recombinant inbred line (RIL) population of 188 lines	529:586	This recombinant inbred line (RIL) population of 188 lines was sequenced at 0.3× depth.
29618164	0	25	theme	sucrose	62:68	arg1	content					70:76	sucrose content	62:76	sucrose content	62:76	Dissecting genomic hotspots underlying seed protein, oil, and sucrose content in an interspecific mapping population of soybean using high-density linkage mapping.
29618164	5	26	theme	PI	516:517	arg1	G. soja					507:513	G. soja	507:513	G. soja	507:513	In this study, an interspecific population was derived from a cross between G. max (Williams 82) and G. soja (PI 483460B).
29618164	5	26	theme	PI	516:517	arg1	483460B					519:525	PI 483460B	516:525	PI 483460B	516:525	In this study, an interspecific population was derived from a cross between G. max (Williams 82) and G. soja (PI 483460B).
29618164	14	27	theme	novel	1579:1583	arg1	QTL					1595:1597	a novel and major QTL	1577:1597	a novel and major QTL	1577:1597	In terms of sucrose content, a novel and major QTL were identified on Chr.
29618164	17	28	theme	cultivated	1987:1996	arg1	soybean					1998:2004	cultivated soybean	1987:2004	cultivated soybean	1987:2004	A QTL-based haplotype using whole-genome resequencing of 106 diverse soybean lines identified unique allelic variation in wild soybean that could be utilized to widen the genetic base in cultivated soybean.
29618164	6	29	theme	0.3×	605:608	arg1	depth					610:614	0.3× depth	605:614	0.3× depth	605:614	This recombinant inbred line (RIL) population of 188 lines was sequenced at 0.3× depth.
29618164	0	30	theme	interspecific	84:96	arg1	population					106:115	an interspecific mapping population	81:115	an interspecific mapping population of soybean	81:126	Dissecting genomic hotspots underlying seed protein, oil, and sucrose content in an interspecific mapping population of soybean using high-density linkage mapping.
29618164	17	31	from	variation	1909:1917	arg1	soybean					1927:1933	wild soybean	1922:1933	wild soybean	1922:1933	A QTL-based haplotype using whole-genome resequencing of 106 diverse soybean lines identified unique allelic variation in wild soybean that could be utilized to widen the genetic base in cultivated soybean.
29618164	8	32	theme	polymorphic	895:905	arg1	3K-SNP					913:918	3K-SNP	913:918	3K-SNP	913:918	In addition to bin mapping, quantitative trait loci (QTL) analysis for protein, oil, and sucrose was performed using 3343 polymorphic SNPs (3K-SNP), derived from Illumina Infinium BeadChip sequencing platform.
29618164	8	32	theme	polymorphic	895:905	arg1	SNPs					907:910	3343 polymorphic SNPs	890:910	3343 polymorphic SNPs (3K-SNP)	890:919	In addition to bin mapping, quantitative trait loci (QTL) analysis for protein, oil, and sucrose was performed using 3343 polymorphic SNPs (3K-SNP), derived from Illumina Infinium BeadChip sequencing platform.
29618164	16	33	theme	91 342	1735:1740	arg1	SNPs					1742:1745	91 342 SNPs	1735:1745	91 342 SNPs	1735:1745	In addition, genome-wide association using 91 342 SNPs confirmed the genomic loci derived from QTL mapping.
29618164	12	34	theme	major	1425:1429	arg1	QTL					1431:1433	The major QTL	1421:1433	The major QTL for protein and oil	1421:1453	The major QTL for protein and oil were mapped on Chr.
29618164	6	35	theme	lines	582:586	arg1	population					564:573	This recombinant inbred line (RIL) population	529:573	This recombinant inbred line (RIL) population of 188 lines	529:586	This recombinant inbred line (RIL) population of 188 lines was sequenced at 0.3× depth.
29618164	14	36	theme	content	1568:1574	arg1	terms					1551:1555	terms	1551:1555	terms of sucrose content	1551:1574	In terms of sucrose content, a novel and major QTL were identified on Chr.
29618164	5	37	attach	derived	453:459	arg1	cross					468:472	a cross	466:472	a cross between G. max (Williams 82) and G. soja (PI 483460B)	466:526	In this study, an interspecific population was derived from a cross between G. max (Williams 82) and G. soja (PI 483460B).
29618164	5	37	attach	derived	453:459	arg2	population					438:447	an interspecific population	421:447	an interspecific population	421:447	In this study, an interspecific population was derived from a cross between G. max (Williams 82) and G. soja (PI 483460B).
29618164	17	38	theme	soybean	1869:1875	arg1	lines					1877:1881	106 diverse soybean lines	1857:1881	106 diverse soybean lines	1857:1881	A QTL-based haplotype using whole-genome resequencing of 106 diverse soybean lines identified unique allelic variation in wild soybean that could be utilized to widen the genetic base in cultivated soybean.
29618164	10	39	theme	bin	1126:1128	arg1	map					1130:1132	the bin map	1122:1132	the bin map derived from next-generation sequencing technology	1122:1183	Importantly, the bin map derived from next-generation sequencing technology enhanced mapping resolution (from 1325 to 50 Kb).
29618164	0	40	theme	seed	39:42	arg1	protein					44:50	seed protein	39:50	seed protein	39:50	Dissecting genomic hotspots underlying seed protein, oil, and sucrose content in an interspecific mapping population of soybean using high-density linkage mapping.
29618164	8	41	theme	bin	788:790	arg1	mapping					792:798	bin mapping	788:798	bin mapping	788:798	In addition to bin mapping, quantitative trait loci (QTL) analysis for protein, oil, and sucrose was performed using 3343 polymorphic SNPs (3K-SNP), derived from Illumina Infinium BeadChip sequencing platform.
29618164	4	42	contain	contains	356:363	arg2	sucrose					397:403	high sucrose	392:403	high sucrose	392:403	Compared to wild soybean, cultivated soybean contains low protein, high oil, and high sucrose.
29618164	4	42	contain	contains	356:363	arg1	soybean					348:354	cultivated soybean	337:354	cultivated soybean	337:354	Compared to wild soybean, cultivated soybean contains low protein, high oil, and high sucrose.
29618164	4	42	contain	contains	356:363	arg2	oil					383:385	high oil	378:385	high oil	378:385	Compared to wild soybean, cultivated soybean contains low protein, high oil, and high sucrose.
29618164	4	42	contain	contains	356:363	arg2	protein					369:375	low protein	365:375	low protein	365:375	Compared to wild soybean, cultivated soybean contains low protein, high oil, and high sucrose.
29618164	11	43	theme	soybean	1377:1383	arg1	loci					1415:1418	soybean domestication-related genomic loci	1377:1418	soybean domestication-related genomic loci	1377:1418	A total of five, nine, and four QTLs were identified for protein, oil, and sucrose content, respectively, and some of the QTLs coincided with soybean domestication-related genomic loci.
29618164	4	44	theme	wild	323:326	arg1	soybean					328:334	wild soybean	323:334	wild soybean	323:334	Compared to wild soybean, cultivated soybean contains low protein, high oil, and high sucrose.
29618164	17	45	theme	allelic	1901:1907	arg1	variation					1909:1917	unique allelic variation	1894:1917	unique allelic variation in wild soybean that could be utilized to widen the genetic base in cultivated soybean	1894:2004	A QTL-based haplotype using whole-genome resequencing of 106 diverse soybean lines identified unique allelic variation in wild soybean that could be utilized to widen the genetic base in cultivated soybean.
29618164	10	46	theme	next-generation	1147:1161	arg1	technology					1174:1183	next-generation sequencing technology	1147:1183	next-generation sequencing technology	1147:1183	Importantly, the bin map derived from next-generation sequencing technology enhanced mapping resolution (from 1325 to 50 Kb).
29618164	8	47	theme	Illumina	935:942	arg1	platform					973:980	Illumina Infinium BeadChip sequencing platform	935:980	Illumina Infinium BeadChip sequencing platform	935:980	In addition to bin mapping, quantitative trait loci (QTL) analysis for protein, oil, and sucrose was performed using 3343 polymorphic SNPs (3K-SNP), derived from Illumina Infinium BeadChip sequencing platform.
29618164	8	48	theme	trait	814:818	arg1	QTL					826:828	QTL	826:828	QTL	826:828	In addition to bin mapping, quantitative trait loci (QTL) analysis for protein, oil, and sucrose was performed using 3343 polymorphic SNPs (3K-SNP), derived from Illumina Infinium BeadChip sequencing platform.
29618164	8	48	theme	trait	814:818	arg1	loci					820:823	quantitative trait loci	801:823	quantitative trait loci (QTL) analysis for protein, oil, and sucrose	801:868	In addition to bin mapping, quantitative trait loci (QTL) analysis for protein, oil, and sucrose was performed using 3343 polymorphic SNPs (3K-SNP), derived from Illumina Infinium BeadChip sequencing platform.
29618164	16	49	attach	derived	1774:1780	arg1	mapping					1791:1797	QTL mapping	1787:1797	QTL mapping	1787:1797	In addition, genome-wide association using 91 342 SNPs confirmed the genomic loci derived from QTL mapping.
29618164	16	49	attach	derived	1774:1780	arg2	loci					1769:1772	the genomic loci	1757:1772	the genomic loci derived from QTL mapping	1757:1797	In addition, genome-wide association using 91 342 SNPs confirmed the genomic loci derived from QTL mapping.
29618164	9	50	theme	QTL	987:989	arg1	regions					991:997	The QTL regions	983:997	The QTL regions from both platforms	983:1017	The QTL regions from both platforms were compared, and a significant concordance was observed between bin and 3K-SNP markers.
29618164	7	51	theme	single	633:638	arg1	SNPs					666:669	SNPs	666:669	SNPs	666:669	Based on 91 342 single nucleotide polymorphisms (SNPs), recombination events in RILs were defined, and a high-resolution bin map was developed (4070 bins).
29618164	7	51	theme	single	633:638	arg1	polymorphisms					651:663	91 342 single nucleotide polymorphisms	626:663	91 342 single nucleotide polymorphisms (SNPs)	626:670	Based on 91 342 single nucleotide polymorphisms (SNPs), recombination events in RILs were defined, and a high-resolution bin map was developed (4070 bins).
29618164	8	52	theme	BeadChip	953:960	arg1	platform					973:980	Illumina Infinium BeadChip sequencing platform	935:980	Illumina Infinium BeadChip sequencing platform	935:980	In addition to bin mapping, quantitative trait loci (QTL) analysis for protein, oil, and sucrose was performed using 3343 polymorphic SNPs (3K-SNP), derived from Illumina Infinium BeadChip sequencing platform.
29618164	10	53	theme	mapping	1194:1200	arg1	resolution					1202:1211	mapping resolution	1194:1211	mapping resolution	1194:1211	Importantly, the bin map derived from next-generation sequencing technology enhanced mapping resolution (from 1325 to 50 Kb).
29618164	4	54	theme	low	365:367	arg1	protein					369:375	low protein	365:375	low protein	365:375	Compared to wild soybean, cultivated soybean contains low protein, high oil, and high sucrose.
29618164	10	55	dep	50 Kb	1227:1231	arg1	1325					1219:1222	1325	1219:1222	1325	1219:1222	Importantly, the bin map derived from next-generation sequencing technology enhanced mapping resolution (from 1325 to 50 Kb).
29618164	11	56	theme	sucrose	1310:1316	arg1	content					1318:1324	sucrose content	1310:1324	sucrose content	1310:1324	A total of five, nine, and four QTLs were identified for protein, oil, and sucrose content, respectively, and some of the QTLs coincided with soybean domestication-related genomic loci.
29618164	17	57	theme	haplotype	1812:1820	arg1	resequencing					1841:1852	A QTL-based haplotype using whole-genome resequencing	1800:1852	A QTL-based haplotype using whole-genome resequencing of 106 diverse soybean lines	1800:1881	A QTL-based haplotype using whole-genome resequencing of 106 diverse soybean lines identified unique allelic variation in wild soybean that could be utilized to widen the genetic base in cultivated soybean.
29618164	7	58	theme	bin	738:740	arg1	map					742:744	a high-resolution bin map	720:744	a high-resolution bin map	720:744	Based on 91 342 single nucleotide polymorphisms (SNPs), recombination events in RILs were defined, and a high-resolution bin map was developed (4070 bins).
29618164	1	59	theme	[Glycine	179:186	arg1	Merr					196:199	The cultivated [Glycine max (L) Merr	164:199	The cultivated [Glycine max (L) Merr.	164:200	The cultivated [Glycine max (L) Merr.]
29618164	17	60	theme	whole-genome	1828:1839	arg1	resequencing					1841:1852	A QTL-based haplotype using whole-genome resequencing	1800:1852	A QTL-based haplotype using whole-genome resequencing of 106 diverse soybean lines	1800:1881	A QTL-based haplotype using whole-genome resequencing of 106 diverse soybean lines identified unique allelic variation in wild soybean that could be utilized to widen the genetic base in cultivated soybean.
29618164	9	61	theme	significant	1040:1050	arg1	concordance					1052:1062	a significant concordance	1038:1062	a significant concordance	1038:1062	The QTL regions from both platforms were compared, and a significant concordance was observed between bin and 3K-SNP markers.
29618164	3	62	theme	soybean	243:249	arg1	species					251:257	soybean species	243:257	soybean species	243:257	soybean species comprise wide variation in seed composition traits.
29618164	2	63	theme	wild	207:210	arg1	soja					221:224	wild [Glycine soja	207:224	wild [Glycine soja	207:224	and wild [Glycine soja Siebold & Zucc.]
29618164	17	64	theme	diverse	1861:1867	arg1	lines					1877:1881	106 diverse soybean lines	1857:1881	106 diverse soybean lines	1857:1881	A QTL-based haplotype using whole-genome resequencing of 106 diverse soybean lines identified unique allelic variation in wild soybean that could be utilized to widen the genetic base in cultivated soybean.
29618164	13	65	theme	negative	1502:1509	arg1	correlation					1511:1521	negative correlation	1502:1521	negative correlation between oil and protein	1502:1545	20 (qPro_20) and suggested negative correlation between oil and protein.
29618164	0	66	theme	linkage	147:153	arg1	mapping					155:161	high-density linkage mapping	134:161	high-density linkage mapping	134:161	Dissecting genomic hotspots underlying seed protein, oil, and sucrose content in an interspecific mapping population of soybean using high-density linkage mapping.
29618164	17	67	used	utilized	1949:1956	arg2	variation					1909:1917	unique allelic variation	1894:1917	unique allelic variation in wild soybean that could be utilized to widen the genetic base in cultivated soybean	1894:2004	A QTL-based haplotype using whole-genome resequencing of 106 diverse soybean lines identified unique allelic variation in wild soybean that could be utilized to widen the genetic base in cultivated soybean.
29618164	6	68	theme	RIL	559:561	arg1	population					564:573	This recombinant inbred line (RIL) population	529:573	This recombinant inbred line (RIL) population of 188 lines	529:586	This recombinant inbred line (RIL) population of 188 lines was sequenced at 0.3× depth.
29618164	17	69	theme	genetic	1971:1977	arg1	base					1979:1982	the genetic base	1967:1982	the genetic base in cultivated soybean	1967:2004	A QTL-based haplotype using whole-genome resequencing of 106 diverse soybean lines identified unique allelic variation in wild soybean that could be utilized to widen the genetic base in cultivated soybean.
29618164	6	70	theme	line	553:556	arg1	population					564:573	This recombinant inbred line (RIL) population	529:573	This recombinant inbred line (RIL) population of 188 lines	529:586	This recombinant inbred line (RIL) population of 188 lines was sequenced at 0.3× depth.
29618164	3	71	theme	seed	286:289	arg1	traits					303:308	seed composition traits	286:308	seed composition traits	286:308	soybean species comprise wide variation in seed composition traits.
29618164	9	72	from	platforms	1009:1017	arg1	regions					991:997	The QTL regions	983:997	The QTL regions from both platforms	983:1017	The QTL regions from both platforms were compared, and a significant concordance was observed between bin and 3K-SNP markers.
29618164	16	73	theme	genome-wide	1705:1715	arg1	association					1717:1727	genome-wide association	1705:1727	genome-wide association using 91 342 SNPs	1705:1745	In addition, genome-wide association using 91 342 SNPs confirmed the genomic loci derived from QTL mapping.
29618164	0	74	theme	mapping	98:104	arg1	population					106:115	an interspecific mapping population	81:115	an interspecific mapping population of soybean	81:126	Dissecting genomic hotspots underlying seed protein, oil, and sucrose content in an interspecific mapping population of soybean using high-density linkage mapping.
29618164	15	75	theme	sugar	1675:1679	arg1	transport					1681:1689	sugar transport	1675:1689	sugar transport	1675:1689	8 (qSuc_08) and harbours putative genes involved in sugar transport.
29618164	0	76	theme	genomic	11:17	arg1	hotspots					19:26	genomic hotspots	11:26	genomic hotspots underlying seed protein, oil, and sucrose content in an interspecific mapping population of soybean	11:126	Dissecting genomic hotspots underlying seed protein, oil, and sucrose content in an interspecific mapping population of soybean using high-density linkage mapping.
29618164	11	77	theme	QTLs	1267:1270	arg1	total					1237:1241	A total	1235:1241	A total of five, nine, and four QTLs	1235:1270	A total of five, nine, and four QTLs were identified for protein, oil, and sucrose content, respectively, and some of the QTLs coincided with soybean domestication-related genomic loci.
29618164	16	78	theme	genomic	1761:1767	arg1	loci					1769:1772	the genomic loci	1757:1772	the genomic loci derived from QTL mapping	1757:1797	In addition, genome-wide association using 91 342 SNPs confirmed the genomic loci derived from QTL mapping.
29618164	6	79	theme	188	578:580	arg1	lines					582:586	188 lines	578:586	188 lines	578:586	This recombinant inbred line (RIL) population of 188 lines was sequenced at 0.3× depth.
29618164	14	80	theme	sucrose	1560:1566	arg1	content					1568:1574	sucrose content	1560:1574	sucrose content	1560:1574	In terms of sucrose content, a novel and major QTL were identified on Chr.
29618164	17	81	theme	lines	1877:1881	arg1	resequencing					1841:1852	A QTL-based haplotype using whole-genome resequencing	1800:1852	A QTL-based haplotype using whole-genome resequencing of 106 diverse soybean lines	1800:1881	A QTL-based haplotype using whole-genome resequencing of 106 diverse soybean lines identified unique allelic variation in wild soybean that could be utilized to widen the genetic base in cultivated soybean.
29618164	7	82	theme	recombination	673:685	arg1	events					687:692	recombination events	673:692	recombination events in RILs	673:700	Based on 91 342 single nucleotide polymorphisms (SNPs), recombination events in RILs were defined, and a high-resolution bin map was developed (4070 bins).
29618164	16	83	theme	QTL	1787:1789	arg1	mapping					1791:1797	QTL mapping	1787:1797	QTL mapping	1787:1797	In addition, genome-wide association using 91 342 SNPs confirmed the genomic loci derived from QTL mapping.
29618164	17	84	theme	unique	1894:1899	arg1	variation					1909:1917	unique allelic variation	1894:1917	unique allelic variation in wild soybean that could be utilized to widen the genetic base in cultivated soybean	1894:2004	A QTL-based haplotype using whole-genome resequencing of 106 diverse soybean lines identified unique allelic variation in wild soybean that could be utilized to widen the genetic base in cultivated soybean.
29618164	10	85	theme	sequencing	1163:1172	arg1	technology					1174:1183	next-generation sequencing technology	1147:1183	next-generation sequencing technology	1147:1183	Importantly, the bin map derived from next-generation sequencing technology enhanced mapping resolution (from 1325 to 50 Kb).
29618164	17	86	theme	wild	1922:1925	arg1	soybean					1927:1933	wild soybean	1922:1933	wild soybean	1922:1933	A QTL-based haplotype using whole-genome resequencing of 106 diverse soybean lines identified unique allelic variation in wild soybean that could be utilized to widen the genetic base in cultivated soybean.
29618164	8	87	theme	Infinium	944:951	arg1	platform					973:980	Illumina Infinium BeadChip sequencing platform	935:980	Illumina Infinium BeadChip sequencing platform	935:980	In addition to bin mapping, quantitative trait loci (QTL) analysis for protein, oil, and sucrose was performed using 3343 polymorphic SNPs (3K-SNP), derived from Illumina Infinium BeadChip sequencing platform.
29618164	8	88	theme	sequencing	962:971	arg1	platform					973:980	Illumina Infinium BeadChip sequencing platform	935:980	Illumina Infinium BeadChip sequencing platform	935:980	In addition to bin mapping, quantitative trait loci (QTL) analysis for protein, oil, and sucrose was performed using 3343 polymorphic SNPs (3K-SNP), derived from Illumina Infinium BeadChip sequencing platform.
29618164	8	89	theme	quantitative	801:812	arg1	QTL					826:828	QTL	826:828	QTL	826:828	In addition to bin mapping, quantitative trait loci (QTL) analysis for protein, oil, and sucrose was performed using 3343 polymorphic SNPs (3K-SNP), derived from Illumina Infinium BeadChip sequencing platform.
29618164	8	89	theme	quantitative	801:812	arg1	loci					820:823	quantitative trait loci	801:823	quantitative trait loci (QTL) analysis for protein, oil, and sucrose	801:868	In addition to bin mapping, quantitative trait loci (QTL) analysis for protein, oil, and sucrose was performed using 3343 polymorphic SNPs (3K-SNP), derived from Illumina Infinium BeadChip sequencing platform.
29618164	8	90	attach	derived	922:928	arg2	SNPs					907:910	3343 polymorphic SNPs	890:910	3343 polymorphic SNPs (3K-SNP)	890:919	In addition to bin mapping, quantitative trait loci (QTL) analysis for protein, oil, and sucrose was performed using 3343 polymorphic SNPs (3K-SNP), derived from Illumina Infinium BeadChip sequencing platform.
29618164	8	90	attach	derived	922:928	arg1	platform					973:980	Illumina Infinium BeadChip sequencing platform	935:980	Illumina Infinium BeadChip sequencing platform	935:980	In addition to bin mapping, quantitative trait loci (QTL) analysis for protein, oil, and sucrose was performed using 3343 polymorphic SNPs (3K-SNP), derived from Illumina Infinium BeadChip sequencing platform.
29618164	8	90	attach	derived	922:928	arg2	3K-SNP					913:918	3K-SNP	913:918	3K-SNP	913:918	In addition to bin mapping, quantitative trait loci (QTL) analysis for protein, oil, and sucrose was performed using 3343 polymorphic SNPs (3K-SNP), derived from Illumina Infinium BeadChip sequencing platform.
29618164	10	91	attach	derived	1134:1140	arg1	technology					1174:1183	next-generation sequencing technology	1147:1183	next-generation sequencing technology	1147:1183	Importantly, the bin map derived from next-generation sequencing technology enhanced mapping resolution (from 1325 to 50 Kb).
29618164	10	91	attach	derived	1134:1140	arg2	map					1130:1132	the bin map	1122:1132	the bin map derived from next-generation sequencing technology	1122:1183	Importantly, the bin map derived from next-generation sequencing technology enhanced mapping resolution (from 1325 to 50 Kb).
29618164	3	92	from	variation	273:281	arg1	traits					303:308	seed composition traits	286:308	seed composition traits	286:308	soybean species comprise wide variation in seed composition traits.
29618164	11	93	theme	domestication-related	1385:1405	arg1	loci					1415:1418	soybean domestication-related genomic loci	1377:1418	soybean domestication-related genomic loci	1377:1418	A total of five, nine, and four QTLs were identified for protein, oil, and sucrose content, respectively, and some of the QTLs coincided with soybean domestication-related genomic loci.
29618164	7	94	theme	nucleotide	640:649	arg1	SNPs					666:669	SNPs	666:669	SNPs	666:669	Based on 91 342 single nucleotide polymorphisms (SNPs), recombination events in RILs were defined, and a high-resolution bin map was developed (4070 bins).
29618164	7	94	theme	nucleotide	640:649	arg1	polymorphisms					651:663	91 342 single nucleotide polymorphisms	626:663	91 342 single nucleotide polymorphisms (SNPs)	626:670	Based on 91 342 single nucleotide polymorphisms (SNPs), recombination events in RILs were defined, and a high-resolution bin map was developed (4070 bins).
31698047	5	0	theme	sld2	765:768	arg1	cells					751:755	the leaf epidermal cells	732:755	the leaf epidermal cells of sld1 sld2	732:768	PD-located protein 5 (PDLP5) was significantly enriched in the leaf epidermal cells of sld1 sld2 and showed specific binding affinity to phytosphinganine (t18:0), suggesting that the enrichment of t18:0-based sphingolipids in sld1 sld2 PDs might facilitate the recruitment of PDLP5 proteins to PDs.
31698047	4	1	theme	double	507:512	arg1	mutant					514:519	the Arabidopsis sld1 sld2 double mutant	481:519	the Arabidopsis sld1 sld2 double mutant	481:519	Here, we report that the Arabidopsis sld1 sld2 double mutant, lacking sphingolipid long-chain base 8 desaturases 1 and 2, displayed decreased PD permeability due to a significant increase in callose accumulation.
31698047	4	2	dep	desaturases	561:571	arg1	1					573:573	1	573:573	1	573:573	Here, we report that the Arabidopsis sld1 sld2 double mutant, lacking sphingolipid long-chain base 8 desaturases 1 and 2, displayed decreased PD permeability due to a significant increase in callose accumulation.
31698047	4	2	dep	desaturases	561:571	arg1	2					579:579	2	579:579	2	579:579	Here, we report that the Arabidopsis sld1 sld2 double mutant, lacking sphingolipid long-chain base 8 desaturases 1 and 2, displayed decreased PD permeability due to a significant increase in callose accumulation.
31698047	5	3	theme	sld1	899:902	arg1	PDs					909:911	sld1 sld2 PDs	899:911	sld1 sld2 PDs	899:911	PD-located protein 5 (PDLP5) was significantly enriched in the leaf epidermal cells of sld1 sld2 and showed specific binding affinity to phytosphinganine (t18:0), suggesting that the enrichment of t18:0-based sphingolipids in sld1 sld2 PDs might facilitate the recruitment of PDLP5 proteins to PDs.
31698047	6	4	theme	mutant	993:998	arg1	seedlings					1000:1008	The sld1 sld2 double mutant seedlings	972:1008	The sld1 sld2 double mutant seedlings	972:1008	The sld1 sld2 double mutant seedlings showed enhanced resistance to the fungal-wilt pathogen Verticillium dahlia and the bacterium Pseudomonas syringae pv.
31698047	7	5	theme	tomato	1128:1133	arg1	DC3000					1135:1140	tomato DC3000	1128:1140	tomato DC3000	1128:1140	tomato DC3000, which could be fully rescued in sld1 sld2 pdlp5 triple mutant.
31698047	5	6	theme	sld2	904:907	arg1	PDs					909:911	sld1 sld2 PDs	899:911	sld1 sld2 PDs	899:911	PD-located protein 5 (PDLP5) was significantly enriched in the leaf epidermal cells of sld1 sld2 and showed specific binding affinity to phytosphinganine (t18:0), suggesting that the enrichment of t18:0-based sphingolipids in sld1 sld2 PDs might facilitate the recruitment of PDLP5 proteins to PDs.
31698047	6	7	theme	double	986:991	arg1	seedlings					1000:1008	The sld1 sld2 double mutant seedlings	972:1008	The sld1 sld2 double mutant seedlings	972:1008	The sld1 sld2 double mutant seedlings showed enhanced resistance to the fungal-wilt pathogen Verticillium dahlia and the bacterium Pseudomonas syringae pv.
31698047	5	8	theme	PD-located	673:682	arg1	PDLP5					695:699	PDLP5	695:699	PDLP5	695:699	PD-located protein 5 (PDLP5) was significantly enriched in the leaf epidermal cells of sld1 sld2 and showed specific binding affinity to phytosphinganine (t18:0), suggesting that the enrichment of t18:0-based sphingolipids in sld1 sld2 PDs might facilitate the recruitment of PDLP5 proteins to PDs.
31698047	5	8	theme	PD-located	673:682	arg1	protein					684:690	PD-located protein 5	673:692	PD-located protein 5 (PDLP5)	673:700	PD-located protein 5 (PDLP5) was significantly enriched in the leaf epidermal cells of sld1 sld2 and showed specific binding affinity to phytosphinganine (t18:0), suggesting that the enrichment of t18:0-based sphingolipids in sld1 sld2 PDs might facilitate the recruitment of PDLP5 proteins to PDs.
31698047	5	9	theme	binding	790:796	arg1	affinity					798:805	specific binding affinity	781:805	specific binding affinity	781:805	PD-located protein 5 (PDLP5) was significantly enriched in the leaf epidermal cells of sld1 sld2 and showed specific binding affinity to phytosphinganine (t18:0), suggesting that the enrichment of t18:0-based sphingolipids in sld1 sld2 PDs might facilitate the recruitment of PDLP5 proteins to PDs.
31698047	5	10	from	enrichment	856:865	arg1	PDs					909:911	sld1 sld2 PDs	899:911	sld1 sld2 PDs	899:911	PD-located protein 5 (PDLP5) was significantly enriched in the leaf epidermal cells of sld1 sld2 and showed specific binding affinity to phytosphinganine (t18:0), suggesting that the enrichment of t18:0-based sphingolipids in sld1 sld2 PDs might facilitate the recruitment of PDLP5 proteins to PDs.
31698047	4	11	theme	PD	602:603	arg1	permeability					605:616	decreased PD permeability	592:616	decreased PD permeability	592:616	Here, we report that the Arabidopsis sld1 sld2 double mutant, lacking sphingolipid long-chain base 8 desaturases 1 and 2, displayed decreased PD permeability due to a significant increase in callose accumulation.
31698047	5	12	theme	leaf	736:739	arg1	cells					751:755	the leaf epidermal cells	732:755	the leaf epidermal cells of sld1 sld2	732:768	PD-located protein 5 (PDLP5) was significantly enriched in the leaf epidermal cells of sld1 sld2 and showed specific binding affinity to phytosphinganine (t18:0), suggesting that the enrichment of t18:0-based sphingolipids in sld1 sld2 PDs might facilitate the recruitment of PDLP5 proteins to PDs.
31698047	4	13	theme	Arabidopsis	485:495	arg1	mutant					514:519	the Arabidopsis sld1 sld2 double mutant	481:519	the Arabidopsis sld1 sld2 double mutant	481:519	Here, we report that the Arabidopsis sld1 sld2 double mutant, lacking sphingolipid long-chain base 8 desaturases 1 and 2, displayed decreased PD permeability due to a significant increase in callose accumulation.
31698047	6	14	theme	sld1	976:979	arg1	seedlings					1000:1008	The sld1 sld2 double mutant seedlings	972:1008	The sld1 sld2 double mutant seedlings	972:1008	The sld1 sld2 double mutant seedlings showed enhanced resistance to the fungal-wilt pathogen Verticillium dahlia and the bacterium Pseudomonas syringae pv.
31698047	6	15	theme	Pseudomonas	1103:1113	arg1	pv					1124:1125	the bacterium Pseudomonas syringae pv	1089:1125	the bacterium Pseudomonas syringae pv	1089:1125	The sld1 sld2 double mutant seedlings showed enhanced resistance to the fungal-wilt pathogen Verticillium dahlia and the bacterium Pseudomonas syringae pv.
31698047	4	16	theme	decreased	592:600	arg1	permeability					605:616	decreased PD permeability	592:616	decreased PD permeability	592:616	Here, we report that the Arabidopsis sld1 sld2 double mutant, lacking sphingolipid long-chain base 8 desaturases 1 and 2, displayed decreased PD permeability due to a significant increase in callose accumulation.
31698047	5	17	theme	epidermal	741:749	arg1	cells					751:755	the leaf epidermal cells	732:755	the leaf epidermal cells of sld1 sld2	732:768	PD-located protein 5 (PDLP5) was significantly enriched in the leaf epidermal cells of sld1 sld2 and showed specific binding affinity to phytosphinganine (t18:0), suggesting that the enrichment of t18:0-based sphingolipids in sld1 sld2 PDs might facilitate the recruitment of PDLP5 proteins to PDs.
31698047	6	18	theme	fungal-wilt	1044:1054	arg1	dahlia					1078:1083	the fungal-wilt pathogen Verticillium dahlia	1040:1083	the fungal-wilt pathogen Verticillium dahlia	1040:1083	The sld1 sld2 double mutant seedlings showed enhanced resistance to the fungal-wilt pathogen Verticillium dahlia and the bacterium Pseudomonas syringae pv.
31698047	1	19	theme	neighboring	208:218	arg1	cells					220:224	neighboring cells	208:224	neighboring cells	208:224	Plant plasmodesmata (PDs) are specialized channels that enable communication between neighboring cells.
31698047	3	20	theme	lipid	374:378	arg1	compositions					380:391	the lipid compositions	370:391	the lipid compositions of PD membrane	370:406	However, the lipid compositions of PD membrane and their impact on PD permeability remain elusive.
31698047	4	21	theme	sld2	502:505	arg1	mutant					514:519	the Arabidopsis sld1 sld2 double mutant	481:519	the Arabidopsis sld1 sld2 double mutant	481:519	Here, we report that the Arabidopsis sld1 sld2 double mutant, lacking sphingolipid long-chain base 8 desaturases 1 and 2, displayed decreased PD permeability due to a significant increase in callose accumulation.
31698047	6	22	theme	bacterium	1093:1101	arg1	pv					1124:1125	the bacterium Pseudomonas syringae pv	1089:1125	the bacterium Pseudomonas syringae pv	1089:1125	The sld1 sld2 double mutant seedlings showed enhanced resistance to the fungal-wilt pathogen Verticillium dahlia and the bacterium Pseudomonas syringae pv.
31698047	7	23	theme	pdlp5	1185:1189	arg1	mutant					1198:1203	sld1 sld2 pdlp5 triple mutant	1175:1203	sld1 sld2 pdlp5 triple mutant	1175:1203	tomato DC3000, which could be fully rescued in sld1 sld2 pdlp5 triple mutant.
31698047	7	24	theme	sld1	1175:1178	arg1	mutant					1198:1203	sld1 sld2 pdlp5 triple mutant	1175:1203	sld1 sld2 pdlp5 triple mutant	1175:1203	tomato DC3000, which could be fully rescued in sld1 sld2 pdlp5 triple mutant.
31698047	4	25	theme	sld1	497:500	arg1	mutant					514:519	the Arabidopsis sld1 sld2 double mutant	481:519	the Arabidopsis sld1 sld2 double mutant	481:519	Here, we report that the Arabidopsis sld1 sld2 double mutant, lacking sphingolipid long-chain base 8 desaturases 1 and 2, displayed decreased PD permeability due to a significant increase in callose accumulation.
31698047	1	26	theme	Plant	123:127	arg1	plasmodesmata					129:141	Plant plasmodesmata	123:141	Plant plasmodesmata (PDs)	123:147	Plant plasmodesmata (PDs) are specialized channels that enable communication between neighboring cells.
31698047	1	26	theme	Plant	123:127	arg1	channels					165:172	specialized channels	153:172	specialized channels that enable communication between neighboring cells	153:224	Plant plasmodesmata (PDs) are specialized channels that enable communication between neighboring cells.
31698047	1	26	theme	Plant	123:127	arg1	PDs					144:146	PDs	144:146	PDs	144:146	Plant plasmodesmata (PDs) are specialized channels that enable communication between neighboring cells.
31698047	7	27	theme	sld2	1180:1183	arg1	mutant					1198:1203	sld1 sld2 pdlp5 triple mutant	1175:1203	sld1 sld2 pdlp5 triple mutant	1175:1203	tomato DC3000, which could be fully rescued in sld1 sld2 pdlp5 triple mutant.
31698047	8	28	from	level	1343:1347	arg1	membranes					1364:1372	PD membranes	1361:1372	PD membranes	1361:1372	Taken together, these results indicate that phytosphinganine might regulate PD functions and cell-to-cell communication by modifying the level of PDLP5 in PD membranes.
31698047	5	29	theme	t18:0-based	870:880	arg1	sphingolipids					882:894	t18:0-based sphingolipids	870:894	t18:0-based sphingolipids	870:894	PD-located protein 5 (PDLP5) was significantly enriched in the leaf epidermal cells of sld1 sld2 and showed specific binding affinity to phytosphinganine (t18:0), suggesting that the enrichment of t18:0-based sphingolipids in sld1 sld2 PDs might facilitate the recruitment of PDLP5 proteins to PDs.
31698047	3	30	theme	PD	396:397	arg1	membrane					399:406	PD membrane	396:406	PD membrane	396:406	However, the lipid compositions of PD membrane and their impact on PD permeability remain elusive.
31698047	5	31	theme	PDLP5	949:953	arg1	proteins					955:962	PDLP5 proteins	949:962	PDLP5 proteins	949:962	PD-located protein 5 (PDLP5) was significantly enriched in the leaf epidermal cells of sld1 sld2 and showed specific binding affinity to phytosphinganine (t18:0), suggesting that the enrichment of t18:0-based sphingolipids in sld1 sld2 PDs might facilitate the recruitment of PDLP5 proteins to PDs.
31698047	6	32	dep	Pseudomonas	1103:1113	arg1	syringae					1115:1122	syringae	1115:1122	syringae	1115:1122	The sld1 sld2 double mutant seedlings showed enhanced resistance to the fungal-wilt pathogen Verticillium dahlia and the bacterium Pseudomonas syringae pv.
31698047	6	33	theme	pathogen	1056:1063	arg1	dahlia					1078:1083	the fungal-wilt pathogen Verticillium dahlia	1040:1083	the fungal-wilt pathogen Verticillium dahlia	1040:1083	The sld1 sld2 double mutant seedlings showed enhanced resistance to the fungal-wilt pathogen Verticillium dahlia and the bacterium Pseudomonas syringae pv.
31698047	3	34	theme	membrane	399:406	arg1	compositions					380:391	the lipid compositions	370:391	the lipid compositions of PD membrane	370:406	However, the lipid compositions of PD membrane and their impact on PD permeability remain elusive.
31698047	3	34	theme	membrane	399:406	arg1	impact					418:423	their impact	412:423	their impact on PD permeability	412:442	However, the lipid compositions of PD membrane and their impact on PD permeability remain elusive.
31698047	5	35	theme	proteins	955:962	arg1	recruitment					934:944	the recruitment	930:944	the recruitment of PDLP5 proteins to PDs	930:969	PD-located protein 5 (PDLP5) was significantly enriched in the leaf epidermal cells of sld1 sld2 and showed specific binding affinity to phytosphinganine (t18:0), suggesting that the enrichment of t18:0-based sphingolipids in sld1 sld2 PDs might facilitate the recruitment of PDLP5 proteins to PDs.
31698047	4	36	from	increase	639:646	arg1	accumulation					659:670	callose accumulation	651:670	callose accumulation	651:670	Here, we report that the Arabidopsis sld1 sld2 double mutant, lacking sphingolipid long-chain base 8 desaturases 1 and 2, displayed decreased PD permeability due to a significant increase in callose accumulation.
31698047	4	37	theme	significant	627:637	arg1	increase					639:646	a significant increase	625:646	a significant increase in callose accumulation	625:670	Here, we report that the Arabidopsis sld1 sld2 double mutant, lacking sphingolipid long-chain base 8 desaturases 1 and 2, displayed decreased PD permeability due to a significant increase in callose accumulation.
31698047	3	38	theme	PD	428:429	arg1	permeability					431:442	PD permeability	428:442	PD permeability	428:442	However, the lipid compositions of PD membrane and their impact on PD permeability remain elusive.
31698047	2	39	theme	plant	280:284	arg1	development					286:296	plant development	280:296	plant development	280:296	The intercellular permeability of PDs, which affects plant development, defense, and responses to stimuli, must be tightly regulated.
31698047	6	40	theme	Verticillium	1065:1076	arg1	dahlia					1078:1083	the fungal-wilt pathogen Verticillium dahlia	1040:1083	the fungal-wilt pathogen Verticillium dahlia	1040:1083	The sld1 sld2 double mutant seedlings showed enhanced resistance to the fungal-wilt pathogen Verticillium dahlia and the bacterium Pseudomonas syringae pv.
31698047	4	41	theme	callose	651:657	arg1	accumulation					659:670	callose accumulation	651:670	callose accumulation	651:670	Here, we report that the Arabidopsis sld1 sld2 double mutant, lacking sphingolipid long-chain base 8 desaturases 1 and 2, displayed decreased PD permeability due to a significant increase in callose accumulation.
31698047	5	42	theme	specific	781:788	arg1	affinity					798:805	specific binding affinity	781:805	specific binding affinity	781:805	PD-located protein 5 (PDLP5) was significantly enriched in the leaf epidermal cells of sld1 sld2 and showed specific binding affinity to phytosphinganine (t18:0), suggesting that the enrichment of t18:0-based sphingolipids in sld1 sld2 PDs might facilitate the recruitment of PDLP5 proteins to PDs.
31698047	4	43	theme	long-chain	543:552	arg1	desaturases					561:571	sphingolipid long-chain base 8 desaturases 1 and 2	530:579	sphingolipid long-chain base 8 desaturases 1 and 2	530:579	Here, we report that the Arabidopsis sld1 sld2 double mutant, lacking sphingolipid long-chain base 8 desaturases 1 and 2, displayed decreased PD permeability due to a significant increase in callose accumulation.
31698047	3	44	from	compositions	380:391	arg1	permeability					431:442	PD permeability	428:442	PD permeability	428:442	However, the lipid compositions of PD membrane and their impact on PD permeability remain elusive.
31698047	7	45	theme	triple	1191:1196	arg1	mutant					1198:1203	sld1 sld2 pdlp5 triple mutant	1175:1203	sld1 sld2 pdlp5 triple mutant	1175:1203	tomato DC3000, which could be fully rescued in sld1 sld2 pdlp5 triple mutant.
31698047	0	46	theme	Callose	86:92	arg1	Accumulation					94:105	PDLP5-Stimulated Callose Accumulation	69:105	PDLP5-Stimulated Callose Accumulation	69:105	Phytosphinganine Affects Plasmodesmata Permeability via Facilitating PDLP5-Stimulated Callose Accumulation in Arabidopsis.
31698047	4	47	theme	sphingolipid	530:541	arg1	desaturases					561:571	sphingolipid long-chain base 8 desaturases 1 and 2	530:579	sphingolipid long-chain base 8 desaturases 1 and 2	530:579	Here, we report that the Arabidopsis sld1 sld2 double mutant, lacking sphingolipid long-chain base 8 desaturases 1 and 2, displayed decreased PD permeability due to a significant increase in callose accumulation.
31698047	0	48	theme	PDLP5-Stimulated	69:84	arg1	Accumulation					94:105	PDLP5-Stimulated Callose Accumulation	69:105	PDLP5-Stimulated Callose Accumulation	69:105	Phytosphinganine Affects Plasmodesmata Permeability via Facilitating PDLP5-Stimulated Callose Accumulation in Arabidopsis.
31698047	2	49	theme	PDs	261:263	arg1	permeability					245:256	The intercellular permeability	227:256	The intercellular permeability	227:256	The intercellular permeability of PDs, which affects plant development, defense, and responses to stimuli, must be tightly regulated.
31698047	8	50	theme	cell-to-cell	1299:1310	arg1	communication					1312:1324	cell-to-cell communication	1299:1324	cell-to-cell communication	1299:1324	Taken together, these results indicate that phytosphinganine might regulate PD functions and cell-to-cell communication by modifying the level of PDLP5 in PD membranes.
31698047	4	51	theme	base	554:557	arg1	desaturases					561:571	sphingolipid long-chain base 8 desaturases 1 and 2	530:579	sphingolipid long-chain base 8 desaturases 1 and 2	530:579	Here, we report that the Arabidopsis sld1 sld2 double mutant, lacking sphingolipid long-chain base 8 desaturases 1 and 2, displayed decreased PD permeability due to a significant increase in callose accumulation.
31698047	1	52	theme	specialized	153:163	arg1	plasmodesmata					129:141	Plant plasmodesmata	123:141	Plant plasmodesmata (PDs)	123:147	Plant plasmodesmata (PDs) are specialized channels that enable communication between neighboring cells.
31698047	1	52	theme	specialized	153:163	arg1	channels					165:172	specialized channels	153:172	specialized channels that enable communication between neighboring cells	153:224	Plant plasmodesmata (PDs) are specialized channels that enable communication between neighboring cells.
31698047	6	53	theme	sld2	981:984	arg1	seedlings					1000:1008	The sld1 sld2 double mutant seedlings	972:1008	The sld1 sld2 double mutant seedlings	972:1008	The sld1 sld2 double mutant seedlings showed enhanced resistance to the fungal-wilt pathogen Verticillium dahlia and the bacterium Pseudomonas syringae pv.
31698047	8	54	theme	PD	1361:1362	arg1	membranes					1364:1372	PD membranes	1361:1372	PD membranes	1361:1372	Taken together, these results indicate that phytosphinganine might regulate PD functions and cell-to-cell communication by modifying the level of PDLP5 in PD membranes.
31698047	8	55	theme	PDLP5	1352:1356	arg1	level					1343:1347	the level	1339:1347	the level of PDLP5 in PD membranes	1339:1372	Taken together, these results indicate that phytosphinganine might regulate PD functions and cell-to-cell communication by modifying the level of PDLP5 in PD membranes.
31698047	6	56	theme	enhanced	1017:1024	arg1	resistance					1026:1035	enhanced resistance	1017:1035	enhanced resistance to the fungal-wilt pathogen Verticillium dahlia and the bacterium Pseudomonas syringae pv	1017:1125	The sld1 sld2 double mutant seedlings showed enhanced resistance to the fungal-wilt pathogen Verticillium dahlia and the bacterium Pseudomonas syringae pv.
31698047	2	57	theme	intercellular	231:243	arg1	permeability					245:256	The intercellular permeability	227:256	The intercellular permeability	227:256	The intercellular permeability of PDs, which affects plant development, defense, and responses to stimuli, must be tightly regulated.
31698047	5	58	theme	sphingolipids	882:894	arg1	enrichment					856:865	the enrichment	852:865	the enrichment of t18:0-based sphingolipids in sld1 sld2 PDs	852:911	PD-located protein 5 (PDLP5) was significantly enriched in the leaf epidermal cells of sld1 sld2 and showed specific binding affinity to phytosphinganine (t18:0), suggesting that the enrichment of t18:0-based sphingolipids in sld1 sld2 PDs might facilitate the recruitment of PDLP5 proteins to PDs.
31698047	5	59	theme	sld1	760:763	arg1	sld2					765:768	sld1 sld2	760:768	sld1 sld2	760:768	PD-located protein 5 (PDLP5) was significantly enriched in the leaf epidermal cells of sld1 sld2 and showed specific binding affinity to phytosphinganine (t18:0), suggesting that the enrichment of t18:0-based sphingolipids in sld1 sld2 PDs might facilitate the recruitment of PDLP5 proteins to PDs.
31698047	3	60	from	impact	418:423	arg1	permeability					431:442	PD permeability	428:442	PD permeability	428:442	However, the lipid compositions of PD membrane and their impact on PD permeability remain elusive.
31698047	0	61	theme	Plasmodesmata	25:37	arg1	Permeability					39:50	Plasmodesmata Permeability	25:50	Plasmodesmata Permeability	25:50	Phytosphinganine Affects Plasmodesmata Permeability via Facilitating PDLP5-Stimulated Callose Accumulation in Arabidopsis.
30885554	5	0	theme	CD44c	747:751	arg1	overexpression					753:766	CD44c overexpression	747:766	CD44c overexpression	747:766	During SVCV infection, the in vivo studies revealed that CD44c overexpression led to the increased virus loads and decreased survival rate.
30885554	0	1	from	regulation	13:22	arg1	infection					64:72	viral and bacterial infection	44:72	viral and bacterial infection	44:72	The negative regulation of piscine CD44c in viral and bacterial infection.
30885554	5	2	theme	survival	815:822	arg1	rate					824:827	survival rate	815:827	survival rate	815:827	During SVCV infection, the in vivo studies revealed that CD44c overexpression led to the increased virus loads and decreased survival rate.
30885554	1	3	theme	alternative	136:146	arg1	splicing					148:155	complex alternative splicing	128:155	complex alternative splicing	128:155	CD44 gene is a cell surface receptor which undergoes complex alternative splicing and extensive post-translational modifications.
30885554	9	4	theme	piscine	1501:1507	arg1	CD44c					1509:1513	piscine CD44c	1501:1513	piscine CD44c	1501:1513	All together, these results firstly demonstrate the negative regulation of piscine CD44c in viral and bacterial infection.
30885554	7	5	theme	larvae	1210:1215	arg1	survival					1217:1224	larvae survival	1210:1224	larvae survival	1210:1224	During Edwardsiella piscicida infection, the overexpression of zebrafish CD44c facilitated bacterial growth and dissemination, but did not impact on larvae survival.
30885554	7	6	from	impact	1200:1205	arg1	survival					1217:1224	larvae survival	1210:1224	larvae survival	1210:1224	During Edwardsiella piscicida infection, the overexpression of zebrafish CD44c facilitated bacterial growth and dissemination, but did not impact on larvae survival.
30885554	8	7	theme	detrimental	1231:1241	arg1	role					1243:1246	The detrimental role	1227:1246	The detrimental role of CD44c in host defense against E. piscicida infection	1227:1302	The detrimental role of CD44c in host defense against E. piscicida infection was supported by a decreased production of several antibacterial molecules including defbl2, defbl3, NK-lysin and RNase3.
30885554	6	8	theme	SVCV	888:891	arg1	infection					893:901	SVCV infection	888:901	SVCV infection	888:901	The attenuated response by zebrafish CD44c in response to SVCV infection were characterized by the impaired production of inflammatory cytokines and the impaired expressions of IFNs, IFN-stimulated genes, MHC class I and II genes.
30885554	4	9	theme	viral	672:676	arg1	infections					678:687	bacterial and viral infections	658:687	bacterial and viral infections	658:687	The expression of zebrafish CD44c was inducible in response to bacterial and viral infections.
30885554	2	10	theme	defense	284:290	arg1	process					268:274	the process	264:274	the process of host defense	264:290	Although many studies have showed that CD44 is involved in the process of host defense, the function of piscine CD44 in antibacterial or antiviral defense response remains unclear.
30885554	2	11	from	function	297:304	arg1	response					360:367	antibacterial or antiviral defense response	325:367	antibacterial or antiviral defense response	325:367	Although many studies have showed that CD44 is involved in the process of host defense, the function of piscine CD44 in antibacterial or antiviral defense response remains unclear.
30885554	6	12	theme	impaired	983:990	arg1	expressions					992:1002	the impaired expressions	979:1002	the impaired expressions of IFNs, IFN-stimulated genes, MHC class I and II genes	979:1058	The attenuated response by zebrafish CD44c in response to SVCV infection were characterized by the impaired production of inflammatory cytokines and the impaired expressions of IFNs, IFN-stimulated genes, MHC class I and II genes.
30885554	3	13	theme	amino	545:549	arg1	composition					556:566	amino acid composition	545:566	amino acid composition	545:566	In the present study, we report the functional characterization of zebrafish CD44c, which is more similar to CD44b antigen isoforms rather than CD44a based on amino acid composition and phylogenetic analysis.
30885554	9	14	theme	CD44c	1509:1513	arg1	regulation					1487:1496	the negative regulation	1474:1496	the negative regulation of piscine CD44c in viral and bacterial infection	1474:1546	All together, these results firstly demonstrate the negative regulation of piscine CD44c in viral and bacterial infection.
30885554	2	15	theme	host	279:282	arg1	defense					284:290	host defense	279:290	host defense	279:290	Although many studies have showed that CD44 is involved in the process of host defense, the function of piscine CD44 in antibacterial or antiviral defense response remains unclear.
30885554	2	16	theme	antiviral	342:350	arg1	response					360:367	antibacterial or antiviral defense response	325:367	antibacterial or antiviral defense response	325:367	Although many studies have showed that CD44 is involved in the process of host defense, the function of piscine CD44 in antibacterial or antiviral defense response remains unclear.
30885554	6	17	from	response	845:852	arg1	response					876:883	response	876:883	response to SVCV infection	876:901	The attenuated response by zebrafish CD44c in response to SVCV infection were characterized by the impaired production of inflammatory cytokines and the impaired expressions of IFNs, IFN-stimulated genes, MHC class I and II genes.
30885554	1	18	theme	cell	90:93	arg1	receptor					103:110	a cell surface receptor	88:110	a cell surface receptor which undergoes complex alternative splicing and extensive post-translational modifications	88:202	CD44 gene is a cell surface receptor which undergoes complex alternative splicing and extensive post-translational modifications.
30885554	1	18	theme	cell	90:93	arg1	gene					80:83	CD44 gene	75:83	CD44 gene	75:83	CD44 gene is a cell surface receptor which undergoes complex alternative splicing and extensive post-translational modifications.
30885554	7	19	theme	piscicida	1081:1089	arg1	infection					1091:1099	Edwardsiella piscicida infection	1068:1099	Edwardsiella piscicida infection	1068:1099	During Edwardsiella piscicida infection, the overexpression of zebrafish CD44c facilitated bacterial growth and dissemination, but did not impact on larvae survival.
30885554	4	20	theme	zebrafish	613:621	arg1	CD44c					623:627	zebrafish CD44c	613:627	zebrafish CD44c	613:627	The expression of zebrafish CD44c was inducible in response to bacterial and viral infections.
30885554	8	21	theme	several	1347:1353	arg1	molecules					1369:1377	several antibacterial molecules	1347:1377	several antibacterial molecules including defbl2, defbl3, NK-lysin and RNase3	1347:1423	The detrimental role of CD44c in host defense against E. piscicida infection was supported by a decreased production of several antibacterial molecules including defbl2, defbl3, NK-lysin and RNase3.
30885554	8	21	theme	several	1347:1353	arg1	NK-lysin					1405:1412	NK-lysin	1405:1412	NK-lysin	1405:1412	The detrimental role of CD44c in host defense against E. piscicida infection was supported by a decreased production of several antibacterial molecules including defbl2, defbl3, NK-lysin and RNase3.
30885554	8	21	theme	several	1347:1353	arg1	defbl3					1397:1402	defbl3	1397:1402	defbl3	1397:1402	The detrimental role of CD44c in host defense against E. piscicida infection was supported by a decreased production of several antibacterial molecules including defbl2, defbl3, NK-lysin and RNase3.
30885554	8	21	theme	several	1347:1353	arg1	RNase3					1418:1423	RNase3	1418:1423	RNase3	1418:1423	The detrimental role of CD44c in host defense against E. piscicida infection was supported by a decreased production of several antibacterial molecules including defbl2, defbl3, NK-lysin and RNase3.
30885554	8	21	theme	several	1347:1353	arg1	defbl2					1389:1394	defbl2	1389:1394	defbl2	1389:1394	The detrimental role of CD44c in host defense against E. piscicida infection was supported by a decreased production of several antibacterial molecules including defbl2, defbl3, NK-lysin and RNase3.
30885554	4	22	theme	bacterial	658:666	arg1	infections					678:687	bacterial and viral infections	658:687	bacterial and viral infections	658:687	The expression of zebrafish CD44c was inducible in response to bacterial and viral infections.
30885554	6	23	theme	class	1039:1043	arg1	genes					1054:1058	MHC class I and II genes	1035:1058	MHC class I and II genes	1035:1058	The attenuated response by zebrafish CD44c in response to SVCV infection were characterized by the impaired production of inflammatory cytokines and the impaired expressions of IFNs, IFN-stimulated genes, MHC class I and II genes.
30885554	6	23	theme	class	1039:1043	arg1	IFNs					1007:1010	IFNs	1007:1010	IFNs	1007:1010	The attenuated response by zebrafish CD44c in response to SVCV infection were characterized by the impaired production of inflammatory cytokines and the impaired expressions of IFNs, IFN-stimulated genes, MHC class I and II genes.
30885554	5	24	theme	increased	779:787	arg1	loads					795:799	the increased virus loads	775:799	the increased virus loads	775:799	During SVCV infection, the in vivo studies revealed that CD44c overexpression led to the increased virus loads and decreased survival rate.
30885554	5	25	theme	virus	789:793	arg1	loads					795:799	the increased virus loads	775:799	the increased virus loads	775:799	During SVCV infection, the in vivo studies revealed that CD44c overexpression led to the increased virus loads and decreased survival rate.
30885554	8	26	theme	E.	1281:1282	arg1	infection					1294:1302	E. piscicida infection	1281:1302	E. piscicida infection	1281:1302	The detrimental role of CD44c in host defense against E. piscicida infection was supported by a decreased production of several antibacterial molecules including defbl2, defbl3, NK-lysin and RNase3.
30885554	4	27	from	response	646:653	arg1	inducible					633:641	inducible	633:641	inducible	633:641	The expression of zebrafish CD44c was inducible in response to bacterial and viral infections.
30885554	4	27	from	response	646:653	arg1	expression					599:608	The expression	595:608	The expression of zebrafish CD44c	595:627	The expression of zebrafish CD44c was inducible in response to bacterial and viral infections.
30885554	1	28	theme	extensive	161:169	arg1	modifications					190:202	extensive post-translational modifications	161:202	extensive post-translational modifications	161:202	CD44 gene is a cell surface receptor which undergoes complex alternative splicing and extensive post-translational modifications.
30885554	0	29	theme	negative	4:11	arg1	regulation					13:22	The negative regulation	0:22	The negative regulation of piscine CD44c in viral and bacterial infection.	0:73	The negative regulation of piscine CD44c in viral and bacterial infection.
30885554	3	30	theme	antigen	501:507	arg1	isoforms					509:516	CD44b antigen isoforms	495:516	CD44b antigen isoforms	495:516	In the present study, we report the functional characterization of zebrafish CD44c, which is more similar to CD44b antigen isoforms rather than CD44a based on amino acid composition and phylogenetic analysis.
30885554	1	31	theme	CD44	75:78	arg1	receptor					103:110	a cell surface receptor	88:110	a cell surface receptor which undergoes complex alternative splicing and extensive post-translational modifications	88:202	CD44 gene is a cell surface receptor which undergoes complex alternative splicing and extensive post-translational modifications.
30885554	1	31	theme	CD44	75:78	arg1	gene					80:83	CD44 gene	75:83	CD44 gene	75:83	CD44 gene is a cell surface receptor which undergoes complex alternative splicing and extensive post-translational modifications.
30885554	1	32	theme	post-translational	171:188	arg1	modifications					190:202	extensive post-translational modifications	161:202	extensive post-translational modifications	161:202	CD44 gene is a cell surface receptor which undergoes complex alternative splicing and extensive post-translational modifications.
30885554	7	33	theme	Edwardsiella	1068:1079	arg1	infection					1091:1099	Edwardsiella piscicida infection	1068:1099	Edwardsiella piscicida infection	1068:1099	During Edwardsiella piscicida infection, the overexpression of zebrafish CD44c facilitated bacterial growth and dissemination, but did not impact on larvae survival.
30885554	9	34	from	regulation	1487:1496	arg1	infection					1538:1546	viral and bacterial infection	1518:1546	viral and bacterial infection	1518:1546	All together, these results firstly demonstrate the negative regulation of piscine CD44c in viral and bacterial infection.
30885554	3	35	theme	acid	551:554	arg1	composition					556:566	amino acid composition	545:566	amino acid composition	545:566	In the present study, we report the functional characterization of zebrafish CD44c, which is more similar to CD44b antigen isoforms rather than CD44a based on amino acid composition and phylogenetic analysis.
30885554	4	36	from	inducible	633:641	arg1	response					646:653	response	646:653	response to bacterial and viral infections	646:687	The expression of zebrafish CD44c was inducible in response to bacterial and viral infections.
30885554	9	37	theme	viral	1518:1522	arg1	infection					1538:1546	viral and bacterial infection	1518:1546	viral and bacterial infection	1518:1546	All together, these results firstly demonstrate the negative regulation of piscine CD44c in viral and bacterial infection.
30885554	6	38	theme	cytokines	965:973	arg1	expressions					992:1002	the impaired expressions	979:1002	the impaired expressions of IFNs, IFN-stimulated genes, MHC class I and II genes	979:1058	The attenuated response by zebrafish CD44c in response to SVCV infection were characterized by the impaired production of inflammatory cytokines and the impaired expressions of IFNs, IFN-stimulated genes, MHC class I and II genes.
30885554	6	38	theme	cytokines	965:973	arg1	production					938:947	the impaired production	925:947	the impaired production of inflammatory cytokines	925:973	The attenuated response by zebrafish CD44c in response to SVCV infection were characterized by the impaired production of inflammatory cytokines and the impaired expressions of IFNs, IFN-stimulated genes, MHC class I and II genes.
30885554	8	39	theme	piscicida	1284:1292	arg1	infection					1294:1302	E. piscicida infection	1281:1302	E. piscicida infection	1281:1302	The detrimental role of CD44c in host defense against E. piscicida infection was supported by a decreased production of several antibacterial molecules including defbl2, defbl3, NK-lysin and RNase3.
30885554	3	40	theme	zebrafish	453:461	arg1	CD44c					463:467	zebrafish CD44c	453:467	zebrafish CD44c	453:467	In the present study, we report the functional characterization of zebrafish CD44c, which is more similar to CD44b antigen isoforms rather than CD44a based on amino acid composition and phylogenetic analysis.
30885554	8	41	theme	molecules	1369:1377	arg1	production					1333:1342	a decreased production	1321:1342	a decreased production of several antibacterial molecules including defbl2, defbl3, NK-lysin and RNase3	1321:1423	The detrimental role of CD44c in host defense against E. piscicida infection was supported by a decreased production of several antibacterial molecules including defbl2, defbl3, NK-lysin and RNase3.
30885554	6	42	theme	inflammatory	952:963	arg1	cytokines					965:973	inflammatory cytokines	952:973	inflammatory cytokines	952:973	The attenuated response by zebrafish CD44c in response to SVCV infection were characterized by the impaired production of inflammatory cytokines and the impaired expressions of IFNs, IFN-stimulated genes, MHC class I and II genes.
30885554	5	43	theme	SVCV	697:700	arg1	infection					702:710	SVCV infection	697:710	SVCV infection	697:710	During SVCV infection, the in vivo studies revealed that CD44c overexpression led to the increased virus loads and decreased survival rate.
30885554	6	44	theme	zebrafish	857:865	arg1	CD44c					867:871	zebrafish CD44c	857:871	zebrafish CD44c	857:871	The attenuated response by zebrafish CD44c in response to SVCV infection were characterized by the impaired production of inflammatory cytokines and the impaired expressions of IFNs, IFN-stimulated genes, MHC class I and II genes.
30885554	9	45	theme	bacterial	1528:1536	arg1	infection					1538:1546	viral and bacterial infection	1518:1546	viral and bacterial infection	1518:1546	All together, these results firstly demonstrate the negative regulation of piscine CD44c in viral and bacterial infection.
30885554	3	46	theme	CD44c	463:467	arg1	similar					484:490	similar	484:490	similar	484:490	In the present study, we report the functional characterization of zebrafish CD44c, which is more similar to CD44b antigen isoforms rather than CD44a based on amino acid composition and phylogenetic analysis.
30885554	3	46	theme	CD44c	463:467	arg1	characterization					433:448	the functional characterization	418:448	the functional characterization	418:448	In the present study, we report the functional characterization of zebrafish CD44c, which is more similar to CD44b antigen isoforms rather than CD44a based on amino acid composition and phylogenetic analysis.
30885554	0	47	theme	CD44c	35:39	arg1	regulation					13:22	The negative regulation	0:22	The negative regulation of piscine CD44c in viral and bacterial infection.	0:73	The negative regulation of piscine CD44c in viral and bacterial infection.
30885554	3	48	theme	phylogenetic	572:583	arg1	analysis					585:592	phylogenetic analysis	572:592	phylogenetic analysis	572:592	In the present study, we report the functional characterization of zebrafish CD44c, which is more similar to CD44b antigen isoforms rather than CD44a based on amino acid composition and phylogenetic analysis.
30885554	6	49	theme	MHC	1035:1037	arg1	class					1039:1043	MHC class I and II	1035:1052	class	1039:1043	The attenuated response by zebrafish CD44c in response to SVCV infection were characterized by the impaired production of inflammatory cytokines and the impaired expressions of IFNs, IFN-stimulated genes, MHC class I and II genes.
30885554	0	50	theme	piscine	27:33	arg1	CD44c					35:39	piscine CD44c	27:39	piscine CD44c	27:39	The negative regulation of piscine CD44c in viral and bacterial infection.
30885554	8	51	from	role	1243:1246	arg1	defense					1265:1271	host defense	1260:1271	host defense against E. piscicida infection	1260:1302	The detrimental role of CD44c in host defense against E. piscicida infection was supported by a decreased production of several antibacterial molecules including defbl2, defbl3, NK-lysin and RNase3.
30885554	7	52	theme	bacterial	1152:1160	arg1	growth					1162:1167	growth	1162:1167	growth	1162:1167	During Edwardsiella piscicida infection, the overexpression of zebrafish CD44c facilitated bacterial growth and dissemination, but did not impact on larvae survival.
30885554	9	53	theme	negative	1478:1485	arg1	regulation					1487:1496	the negative regulation	1474:1496	the negative regulation of piscine CD44c in viral and bacterial infection	1474:1546	All together, these results firstly demonstrate the negative regulation of piscine CD44c in viral and bacterial infection.
30885554	6	54	theme	II	1051:1052	arg1	genes					1054:1058	MHC class I and II genes	1035:1058	MHC class I and II genes	1035:1058	The attenuated response by zebrafish CD44c in response to SVCV infection were characterized by the impaired production of inflammatory cytokines and the impaired expressions of IFNs, IFN-stimulated genes, MHC class I and II genes.
30885554	6	54	theme	II	1051:1052	arg1	IFNs					1007:1010	IFNs	1007:1010	IFNs	1007:1010	The attenuated response by zebrafish CD44c in response to SVCV infection were characterized by the impaired production of inflammatory cytokines and the impaired expressions of IFNs, IFN-stimulated genes, MHC class I and II genes.
30885554	0	55	theme	viral	44:48	arg1	infection					64:72	viral and bacterial infection	44:72	viral and bacterial infection	44:72	The negative regulation of piscine CD44c in viral and bacterial infection.
30885554	6	56	theme	impaired	929:936	arg1	production					938:947	the impaired production	925:947	the impaired production of inflammatory cytokines	925:973	The attenuated response by zebrafish CD44c in response to SVCV infection were characterized by the impaired production of inflammatory cytokines and the impaired expressions of IFNs, IFN-stimulated genes, MHC class I and II genes.
30885554	6	57	theme	attenuated	834:843	arg1	response					845:852	The attenuated response	830:852	The attenuated response by zebrafish CD44c in response to SVCV infection	830:901	The attenuated response by zebrafish CD44c in response to SVCV infection were characterized by the impaired production of inflammatory cytokines and the impaired expressions of IFNs, IFN-stimulated genes, MHC class I and II genes.
30885554	8	58	theme	antibacterial	1355:1367	arg1	molecules					1369:1377	several antibacterial molecules	1347:1377	several antibacterial molecules including defbl2, defbl3, NK-lysin and RNase3	1347:1423	The detrimental role of CD44c in host defense against E. piscicida infection was supported by a decreased production of several antibacterial molecules including defbl2, defbl3, NK-lysin and RNase3.
30885554	8	58	theme	antibacterial	1355:1367	arg1	NK-lysin					1405:1412	NK-lysin	1405:1412	NK-lysin	1405:1412	The detrimental role of CD44c in host defense against E. piscicida infection was supported by a decreased production of several antibacterial molecules including defbl2, defbl3, NK-lysin and RNase3.
30885554	8	58	theme	antibacterial	1355:1367	arg1	defbl3					1397:1402	defbl3	1397:1402	defbl3	1397:1402	The detrimental role of CD44c in host defense against E. piscicida infection was supported by a decreased production of several antibacterial molecules including defbl2, defbl3, NK-lysin and RNase3.
30885554	8	58	theme	antibacterial	1355:1367	arg1	RNase3					1418:1423	RNase3	1418:1423	RNase3	1418:1423	The detrimental role of CD44c in host defense against E. piscicida infection was supported by a decreased production of several antibacterial molecules including defbl2, defbl3, NK-lysin and RNase3.
30885554	8	58	theme	antibacterial	1355:1367	arg1	defbl2					1389:1394	defbl2	1389:1394	defbl2	1389:1394	The detrimental role of CD44c in host defense against E. piscicida infection was supported by a decreased production of several antibacterial molecules including defbl2, defbl3, NK-lysin and RNase3.
30885554	2	59	theme	antibacterial	325:337	arg1	response					360:367	antibacterial or antiviral defense response	325:367	antibacterial or antiviral defense response	325:367	Although many studies have showed that CD44 is involved in the process of host defense, the function of piscine CD44 in antibacterial or antiviral defense response remains unclear.
30885554	3	60	theme	present	393:399	arg1	study					401:405	the present study	389:405	the present study	389:405	In the present study, we report the functional characterization of zebrafish CD44c, which is more similar to CD44b antigen isoforms rather than CD44a based on amino acid composition and phylogenetic analysis.
30885554	1	61	theme	complex	128:134	arg1	splicing					148:155	complex alternative splicing	128:155	complex alternative splicing	128:155	CD44 gene is a cell surface receptor which undergoes complex alternative splicing and extensive post-translational modifications.
30885554	1	62	theme	surface	95:101	arg1	receptor					103:110	a cell surface receptor	88:110	a cell surface receptor which undergoes complex alternative splicing and extensive post-translational modifications	88:202	CD44 gene is a cell surface receptor which undergoes complex alternative splicing and extensive post-translational modifications.
30885554	1	62	theme	surface	95:101	arg1	gene					80:83	CD44 gene	75:83	CD44 gene	75:83	CD44 gene is a cell surface receptor which undergoes complex alternative splicing and extensive post-translational modifications.
30885554	2	63	theme	many	214:217	arg1	studies					219:225	many studies	214:225	many studies	214:225	Although many studies have showed that CD44 is involved in the process of host defense, the function of piscine CD44 in antibacterial or antiviral defense response remains unclear.
30885554	7	64	theme	CD44c	1134:1138	arg1	overexpression					1106:1119	the overexpression	1102:1119	the overexpression of zebrafish CD44c	1102:1138	During Edwardsiella piscicida infection, the overexpression of zebrafish CD44c facilitated bacterial growth and dissemination, but did not impact on larvae survival.
30885554	2	65	theme	CD44	317:320	arg1	function					297:304	the function	293:304	the function of piscine CD44 in antibacterial or antiviral defense response	293:367	Although many studies have showed that CD44 is involved in the process of host defense, the function of piscine CD44 in antibacterial or antiviral defense response remains unclear.
30885554	5	66	dep	in	717:718	arg1	vivo					720:723	vivo	720:723	vivo	720:723	During SVCV infection, the in vivo studies revealed that CD44c overexpression led to the increased virus loads and decreased survival rate.
30885554	2	67	theme	piscine	309:315	arg1	CD44					317:320	piscine CD44	309:320	piscine CD44	309:320	Although many studies have showed that CD44 is involved in the process of host defense, the function of piscine CD44 in antibacterial or antiviral defense response remains unclear.
30885554	8	68	theme	decreased	1323:1331	arg1	production					1333:1342	a decreased production	1321:1342	a decreased production of several antibacterial molecules including defbl2, defbl3, NK-lysin and RNase3	1321:1423	The detrimental role of CD44c in host defense against E. piscicida infection was supported by a decreased production of several antibacterial molecules including defbl2, defbl3, NK-lysin and RNase3.
30885554	6	69	theme	IFN-stimulated	1013:1026	arg1	IFNs					1007:1010	IFNs	1007:1010	IFNs	1007:1010	The attenuated response by zebrafish CD44c in response to SVCV infection were characterized by the impaired production of inflammatory cytokines and the impaired expressions of IFNs, IFN-stimulated genes, MHC class I and II genes.
30885554	6	69	theme	IFN-stimulated	1013:1026	arg1	genes					1028:1032	IFN-stimulated genes	1013:1032	IFN-stimulated genes	1013:1032	The attenuated response by zebrafish CD44c in response to SVCV infection were characterized by the impaired production of inflammatory cytokines and the impaired expressions of IFNs, IFN-stimulated genes, MHC class I and II genes.
30885554	0	70	theme	bacterial	54:62	arg1	infection					64:72	viral and bacterial infection	44:72	viral and bacterial infection	44:72	The negative regulation of piscine CD44c in viral and bacterial infection.
30885554	8	71	theme	host	1260:1263	arg1	defense					1265:1271	host defense	1260:1271	host defense against E. piscicida infection	1260:1302	The detrimental role of CD44c in host defense against E. piscicida infection was supported by a decreased production of several antibacterial molecules including defbl2, defbl3, NK-lysin and RNase3.
30885554	4	72	theme	CD44c	623:627	arg1	inducible					633:641	inducible	633:641	inducible	633:641	The expression of zebrafish CD44c was inducible in response to bacterial and viral infections.
30885554	4	72	theme	CD44c	623:627	arg1	expression					599:608	The expression	595:608	The expression of zebrafish CD44c	595:627	The expression of zebrafish CD44c was inducible in response to bacterial and viral infections.
30885554	3	73	theme	CD44b	495:499	arg1	isoforms					509:516	CD44b antigen isoforms	495:516	CD44b antigen isoforms	495:516	In the present study, we report the functional characterization of zebrafish CD44c, which is more similar to CD44b antigen isoforms rather than CD44a based on amino acid composition and phylogenetic analysis.
30885554	3	74	theme	functional	422:431	arg1	similar					484:490	similar	484:490	similar	484:490	In the present study, we report the functional characterization of zebrafish CD44c, which is more similar to CD44b antigen isoforms rather than CD44a based on amino acid composition and phylogenetic analysis.
30885554	3	74	theme	functional	422:431	arg1	characterization					433:448	the functional characterization	418:448	the functional characterization	418:448	In the present study, we report the functional characterization of zebrafish CD44c, which is more similar to CD44b antigen isoforms rather than CD44a based on amino acid composition and phylogenetic analysis.
30885554	8	75	theme	CD44c	1251:1255	arg1	role					1243:1246	The detrimental role	1227:1246	The detrimental role of CD44c in host defense against E. piscicida infection	1227:1302	The detrimental role of CD44c in host defense against E. piscicida infection was supported by a decreased production of several antibacterial molecules including defbl2, defbl3, NK-lysin and RNase3.
30885554	7	76	theme	zebrafish	1124:1132	arg1	CD44c					1134:1138	zebrafish CD44c	1124:1138	zebrafish CD44c	1124:1138	During Edwardsiella piscicida infection, the overexpression of zebrafish CD44c facilitated bacterial growth and dissemination, but did not impact on larvae survival.
30885554	6	77	theme	IFNs	1007:1010	arg1	expressions					992:1002	the impaired expressions	979:1002	the impaired expressions of IFNs, IFN-stimulated genes, MHC class I and II genes	979:1058	The attenuated response by zebrafish CD44c in response to SVCV infection were characterized by the impaired production of inflammatory cytokines and the impaired expressions of IFNs, IFN-stimulated genes, MHC class I and II genes.
30885554	6	77	theme	IFNs	1007:1010	arg1	production					938:947	the impaired production	925:947	the impaired production of inflammatory cytokines	925:973	The attenuated response by zebrafish CD44c in response to SVCV infection were characterized by the impaired production of inflammatory cytokines and the impaired expressions of IFNs, IFN-stimulated genes, MHC class I and II genes.
30885554	2	78	theme	defense	352:358	arg1	response					360:367	antibacterial or antiviral defense response	325:367	antibacterial or antiviral defense response	325:367	Although many studies have showed that CD44 is involved in the process of host defense, the function of piscine CD44 in antibacterial or antiviral defense response remains unclear.
30885554	5	79	theme	in	717:718	arg1	studies					725:731	the in vivo studies	713:731	the in vivo studies	713:731	During SVCV infection, the in vivo studies revealed that CD44c overexpression led to the increased virus loads and decreased survival rate.
31665888	0	0	theme	Mixed	122:126	arg1	Infections					128:137	Mixed Infections	122:137	Mixed Infections of Wounds	122:147	Quaternized Chitosan-Coated Montmorillonite Interior Antimicrobial Metal-Antibiotic in Situ Coordination Complexation for Mixed Infections of Wounds.
31665888	3	1	theme	coli	773:776	arg1	coordinates					860:870	Staphylococcus aureus (a typical Gram-positive bacterium), Escherichia coli (a typical Gram-negative bacterium), and Candida albicans (a fungus) because 5-FC coordinates	702:870	Staphylococcus aureus (a typical Gram-positive bacterium), Escherichia coli (a typical Gram-negative bacterium), and Candida albicans (a fungus) because 5-FC coordinates with copper ions	702:887	Composite material QCS/MMT/5-FCCu can can strongly inhibit Staphylococcus aureus (a typical Gram-positive bacterium), Escherichia coli (a typical Gram-negative bacterium), and Candida albicans (a fungus) because 5-FC coordinates with copper ions in situ and due to the deposition of QCS.
31665888	1	2	from	face	218:221	arg1	application					262:272	their practical application	246:272	their practical application	246:272	Conventional drug delivery systems for natural clay materials still face critical challenges in their practical application, including multiple bacterial infections, combined infection of bacteria and fungi, and low sterilization efficiency.
31665888	1	3	theme	clay	197:200	arg1	materials					202:210	natural clay materials	189:210	natural clay materials	189:210	Conventional drug delivery systems for natural clay materials still face critical challenges in their practical application, including multiple bacterial infections, combined infection of bacteria and fungi, and low sterilization efficiency.
31665888	3	4	theme	copper	877:882	arg1	ions					884:887	copper ions	877:887	copper ions	877:887	Composite material QCS/MMT/5-FCCu can can strongly inhibit Staphylococcus aureus (a typical Gram-positive bacterium), Escherichia coli (a typical Gram-negative bacterium), and Candida albicans (a fungus) because 5-FC coordinates with copper ions in situ and due to the deposition of QCS.
31665888	2	5	theme	antibiotic	542:551	arg1	5-FC					571:574	5-FC	571:574	5-FC	571:574	In this work, we address these challenges using the multifunctional montmorillonite nanosheet-based (MMT-based) drug nanoplatform, which involves the antibiotic 5-fluorocytosine (5-FC), antibacterial metal copper ions, and quaternized chitosan (QCS).
31665888	2	5	theme	antibiotic	542:551	arg1	5-fluorocytosine					553:568	the antibiotic 5-fluorocytosine	538:568	the antibiotic 5-fluorocytosine (5-FC)	538:575	In this work, we address these challenges using the multifunctional montmorillonite nanosheet-based (MMT-based) drug nanoplatform, which involves the antibiotic 5-fluorocytosine (5-FC), antibacterial metal copper ions, and quaternized chitosan (QCS).
31665888	5	6	theme	excellent	1210:1218	arg1	biocompatibility					1220:1235	excellent biocompatibility	1210:1235	excellent biocompatibility	1210:1235	Moreover, in vivo wound experiments and toxicity experiments show the promotion of wound healing and excellent biocompatibility.
31665888	5	7	theme	in	1119:1120	arg1	experiments					1133:1143	in vivo wound experiments	1119:1143	in vivo wound experiments	1119:1143	Moreover, in vivo wound experiments and toxicity experiments show the promotion of wound healing and excellent biocompatibility.
31665888	2	8	theme	metal	592:596	arg1	ions					605:608	antibacterial metal copper ions	578:608	antibacterial metal copper ions	578:608	In this work, we address these challenges using the multifunctional montmorillonite nanosheet-based (MMT-based) drug nanoplatform, which involves the antibiotic 5-fluorocytosine (5-FC), antibacterial metal copper ions, and quaternized chitosan (QCS).
31665888	5	9	theme	wound	1192:1196	arg1	healing					1198:1204	wound healing	1192:1204	wound healing	1192:1204	Moreover, in vivo wound experiments and toxicity experiments show the promotion of wound healing and excellent biocompatibility.
31665888	6	10	theme	smart	1320:1324	arg1	platform					1326:1333	the MMT-based smart platform	1306:1333	the MMT-based smart platform	1306:1333	As a demonstration of the utility of the latter, we have shown that the MMT-based smart platform can be used for the treatment of mixed infections of wounds.
31665888	1	11	theme	combined	316:323	arg1	infection					325:333	combined infection	316:333	combined infection of bacteria and fungi	316:355	Conventional drug delivery systems for natural clay materials still face critical challenges in their practical application, including multiple bacterial infections, combined infection of bacteria and fungi, and low sterilization efficiency.
31665888	2	12	theme	antibacterial	578:590	arg1	ions					605:608	antibacterial metal copper ions	578:608	antibacterial metal copper ions	578:608	In this work, we address these challenges using the multifunctional montmorillonite nanosheet-based (MMT-based) drug nanoplatform, which involves the antibiotic 5-fluorocytosine (5-FC), antibacterial metal copper ions, and quaternized chitosan (QCS).
31665888	3	13	theme	typical	781:787	arg1	bacterium					803:811	a typical Gram-negative bacterium	779:811	a typical Gram-negative bacterium	779:811	Composite material QCS/MMT/5-FCCu can can strongly inhibit Staphylococcus aureus (a typical Gram-positive bacterium), Escherichia coli (a typical Gram-negative bacterium), and Candida albicans (a fungus) because 5-FC coordinates with copper ions in situ and due to the deposition of QCS.
31665888	3	13	theme	typical	781:787	arg1	coli					773:776	Escherichia coli	761:776	Escherichia coli (a typical Gram-negative bacterium)	761:812	Composite material QCS/MMT/5-FCCu can can strongly inhibit Staphylococcus aureus (a typical Gram-positive bacterium), Escherichia coli (a typical Gram-negative bacterium), and Candida albicans (a fungus) because 5-FC coordinates with copper ions in situ and due to the deposition of QCS.
31665888	4	14	theme	long-acting	1067:1077	arg1	release					1089:1095	long-acting sustained release	1067:1095	long-acting sustained release inhibition	1067:1106	The subsequent drug release behavior of 5-FCCu was studied, and the results show an initial high concentration kills microorganisms and long-acting sustained release inhibition.
31665888	5	15	theme	biocompatibility	1220:1235	arg1	promotion					1179:1187	the promotion	1175:1187	the promotion of wound healing and excellent biocompatibility	1175:1235	Moreover, in vivo wound experiments and toxicity experiments show the promotion of wound healing and excellent biocompatibility.
31665888	4	16	theme	high	1023:1026	arg1	concentration					1028:1040	an initial high concentration	1012:1040	an initial high concentration	1012:1040	The subsequent drug release behavior of 5-FCCu was studied, and the results show an initial high concentration kills microorganisms and long-acting sustained release inhibition.
31665888	6	17	theme	wounds	1388:1393	arg1	infections					1374:1383	mixed infections	1368:1383	mixed infections of wounds	1368:1393	As a demonstration of the utility of the latter, we have shown that the MMT-based smart platform can be used for the treatment of mixed infections of wounds.
31665888	3	18	theme	Gram-negative	789:801	arg1	bacterium					803:811	a typical Gram-negative bacterium	779:811	a typical Gram-negative bacterium	779:811	Composite material QCS/MMT/5-FCCu can can strongly inhibit Staphylococcus aureus (a typical Gram-positive bacterium), Escherichia coli (a typical Gram-negative bacterium), and Candida albicans (a fungus) because 5-FC coordinates with copper ions in situ and due to the deposition of QCS.
31665888	3	18	theme	Gram-negative	789:801	arg1	coli					773:776	Escherichia coli	761:776	Escherichia coli (a typical Gram-negative bacterium)	761:812	Composite material QCS/MMT/5-FCCu can can strongly inhibit Staphylococcus aureus (a typical Gram-positive bacterium), Escherichia coli (a typical Gram-negative bacterium), and Candida albicans (a fungus) because 5-FC coordinates with copper ions in situ and due to the deposition of QCS.
31665888	4	19	theme	5-FCCu	971:976	arg1	behavior					959:966	The subsequent drug release behavior	931:966	The subsequent drug release behavior of 5-FCCu	931:976	The subsequent drug release behavior of 5-FCCu was studied, and the results show an initial high concentration kills microorganisms and long-acting sustained release inhibition.
31665888	0	20	theme	Wounds	142:147	arg1	Infections					128:137	Mixed Infections	122:137	Mixed Infections of Wounds	122:147	Quaternized Chitosan-Coated Montmorillonite Interior Antimicrobial Metal-Antibiotic in Situ Coordination Complexation for Mixed Infections of Wounds.
31665888	3	21	dep	deposition	912:921	arg1	and due to					897:906	situ and due to the deposition of QCS	892:928	situ and due to the deposition of QCS	892:928	Composite material QCS/MMT/5-FCCu can can strongly inhibit Staphylococcus aureus (a typical Gram-positive bacterium), Escherichia coli (a typical Gram-negative bacterium), and Candida albicans (a fungus) because 5-FC coordinates with copper ions in situ and due to the deposition of QCS.
31665888	3	22	theme	albicans	827:834	arg1	coordinates					860:870	Staphylococcus aureus (a typical Gram-positive bacterium), Escherichia coli (a typical Gram-negative bacterium), and Candida albicans (a fungus) because 5-FC coordinates	702:870	Staphylococcus aureus (a typical Gram-positive bacterium), Escherichia coli (a typical Gram-negative bacterium), and Candida albicans (a fungus) because 5-FC coordinates with copper ions	702:887	Composite material QCS/MMT/5-FCCu can can strongly inhibit Staphylococcus aureus (a typical Gram-positive bacterium), Escherichia coli (a typical Gram-negative bacterium), and Candida albicans (a fungus) because 5-FC coordinates with copper ions in situ and due to the deposition of QCS.
31665888	3	23	theme	Candida	819:825	arg1	albicans					827:834	Candida albicans	819:834	Candida albicans	819:834	Composite material QCS/MMT/5-FCCu can can strongly inhibit Staphylococcus aureus (a typical Gram-positive bacterium), Escherichia coli (a typical Gram-negative bacterium), and Candida albicans (a fungus) because 5-FC coordinates with copper ions in situ and due to the deposition of QCS.
31665888	0	24	theme	Chitosan-Coated	12:26	arg1	Complexation					105:116	Chitosan-Coated Montmorillonite Interior Antimicrobial Metal-Antibiotic in Situ Coordination Complexation	12:116	Chitosan-Coated Montmorillonite Interior Antimicrobial Metal-Antibiotic in Situ Coordination Complexation for Mixed Infections of Wounds	12:147	Quaternized Chitosan-Coated Montmorillonite Interior Antimicrobial Metal-Antibiotic in Situ Coordination Complexation for Mixed Infections of Wounds.
31665888	4	25	theme	drug	946:949	arg1	behavior					959:966	The subsequent drug release behavior	931:966	The subsequent drug release behavior of 5-FCCu	931:976	The subsequent drug release behavior of 5-FCCu was studied, and the results show an initial high concentration kills microorganisms and long-acting sustained release inhibition.
31665888	6	26	theme	MMT-based	1310:1318	arg1	platform					1326:1333	the MMT-based smart platform	1306:1333	the MMT-based smart platform	1306:1333	As a demonstration of the utility of the latter, we have shown that the MMT-based smart platform can be used for the treatment of mixed infections of wounds.
31665888	5	27	theme	toxicity	1149:1156	arg1	experiments					1158:1168	toxicity experiments	1149:1168	toxicity experiments	1149:1168	Moreover, in vivo wound experiments and toxicity experiments show the promotion of wound healing and excellent biocompatibility.
31665888	1	28	theme	critical	223:230	arg1	challenges					232:241	critical challenges	223:241	critical challenges	223:241	Conventional drug delivery systems for natural clay materials still face critical challenges in their practical application, including multiple bacterial infections, combined infection of bacteria and fungi, and low sterilization efficiency.
31665888	2	29	theme	drug	504:507	arg1	nanoplatform					509:520	the multifunctional montmorillonite nanosheet-based (MMT-based) drug nanoplatform	440:520	the multifunctional montmorillonite nanosheet-based (MMT-based) drug nanoplatform	440:520	In this work, we address these challenges using the multifunctional montmorillonite nanosheet-based (MMT-based) drug nanoplatform, which involves the antibiotic 5-fluorocytosine (5-FC), antibacterial metal copper ions, and quaternized chitosan (QCS).
31665888	0	30	theme	Interior	44:51	arg1	Complexation					105:116	Chitosan-Coated Montmorillonite Interior Antimicrobial Metal-Antibiotic in Situ Coordination Complexation	12:116	Chitosan-Coated Montmorillonite Interior Antimicrobial Metal-Antibiotic in Situ Coordination Complexation for Mixed Infections of Wounds	12:147	Quaternized Chitosan-Coated Montmorillonite Interior Antimicrobial Metal-Antibiotic in Situ Coordination Complexation for Mixed Infections of Wounds.
31665888	1	31	theme	bacteria	338:345	arg1	efficiency					380:389	low sterilization efficiency	362:389	low sterilization efficiency	362:389	Conventional drug delivery systems for natural clay materials still face critical challenges in their practical application, including multiple bacterial infections, combined infection of bacteria and fungi, and low sterilization efficiency.
31665888	1	31	theme	bacteria	338:345	arg1	infections					304:313	multiple bacterial infections	285:313	multiple bacterial infections	285:313	Conventional drug delivery systems for natural clay materials still face critical challenges in their practical application, including multiple bacterial infections, combined infection of bacteria and fungi, and low sterilization efficiency.
31665888	1	31	theme	bacteria	338:345	arg1	infection					325:333	combined infection	316:333	combined infection of bacteria and fungi	316:355	Conventional drug delivery systems for natural clay materials still face critical challenges in their practical application, including multiple bacterial infections, combined infection of bacteria and fungi, and low sterilization efficiency.
31665888	6	32	theme	infections	1374:1383	arg1	treatment					1355:1363	the treatment	1351:1363	the treatment of mixed infections of wounds	1351:1393	As a demonstration of the utility of the latter, we have shown that the MMT-based smart platform can be used for the treatment of mixed infections of wounds.
31665888	0	33	theme	Montmorillonite	28:42	arg1	Complexation					105:116	Chitosan-Coated Montmorillonite Interior Antimicrobial Metal-Antibiotic in Situ Coordination Complexation	12:116	Chitosan-Coated Montmorillonite Interior Antimicrobial Metal-Antibiotic in Situ Coordination Complexation for Mixed Infections of Wounds	12:147	Quaternized Chitosan-Coated Montmorillonite Interior Antimicrobial Metal-Antibiotic in Situ Coordination Complexation for Mixed Infections of Wounds.
31665888	6	34	theme	mixed	1368:1372	arg1	infections					1374:1383	mixed infections	1368:1383	mixed infections of wounds	1368:1393	As a demonstration of the utility of the latter, we have shown that the MMT-based smart platform can be used for the treatment of mixed infections of wounds.
31665888	3	35	theme	aureus	717:722	arg1	coordinates					860:870	Staphylococcus aureus (a typical Gram-positive bacterium), Escherichia coli (a typical Gram-negative bacterium), and Candida albicans (a fungus) because 5-FC coordinates	702:870	Staphylococcus aureus (a typical Gram-positive bacterium), Escherichia coli (a typical Gram-negative bacterium), and Candida albicans (a fungus) because 5-FC coordinates with copper ions	702:887	Composite material QCS/MMT/5-FCCu can can strongly inhibit Staphylococcus aureus (a typical Gram-positive bacterium), Escherichia coli (a typical Gram-negative bacterium), and Candida albicans (a fungus) because 5-FC coordinates with copper ions in situ and due to the deposition of QCS.
31665888	2	36	theme	nanosheet-based	476:490	arg1	nanoplatform					509:520	the multifunctional montmorillonite nanosheet-based (MMT-based) drug nanoplatform	440:520	the multifunctional montmorillonite nanosheet-based (MMT-based) drug nanoplatform	440:520	In this work, we address these challenges using the multifunctional montmorillonite nanosheet-based (MMT-based) drug nanoplatform, which involves the antibiotic 5-fluorocytosine (5-FC), antibacterial metal copper ions, and quaternized chitosan (QCS).
31665888	0	37	theme	Metal-Antibiotic	67:82	arg1	Complexation					105:116	Chitosan-Coated Montmorillonite Interior Antimicrobial Metal-Antibiotic in Situ Coordination Complexation	12:116	Chitosan-Coated Montmorillonite Interior Antimicrobial Metal-Antibiotic in Situ Coordination Complexation for Mixed Infections of Wounds	12:147	Quaternized Chitosan-Coated Montmorillonite Interior Antimicrobial Metal-Antibiotic in Situ Coordination Complexation for Mixed Infections of Wounds.
31665888	1	38	theme	fungi	351:355	arg1	efficiency					380:389	low sterilization efficiency	362:389	low sterilization efficiency	362:389	Conventional drug delivery systems for natural clay materials still face critical challenges in their practical application, including multiple bacterial infections, combined infection of bacteria and fungi, and low sterilization efficiency.
31665888	1	38	theme	fungi	351:355	arg1	infections					304:313	multiple bacterial infections	285:313	multiple bacterial infections	285:313	Conventional drug delivery systems for natural clay materials still face critical challenges in their practical application, including multiple bacterial infections, combined infection of bacteria and fungi, and low sterilization efficiency.
31665888	1	38	theme	fungi	351:355	arg1	infection					325:333	combined infection	316:333	combined infection of bacteria and fungi	316:355	Conventional drug delivery systems for natural clay materials still face critical challenges in their practical application, including multiple bacterial infections, combined infection of bacteria and fungi, and low sterilization efficiency.
31665888	2	39	theme	montmorillonite	460:474	arg1	nanoplatform					509:520	the multifunctional montmorillonite nanosheet-based (MMT-based) drug nanoplatform	440:520	the multifunctional montmorillonite nanosheet-based (MMT-based) drug nanoplatform	440:520	In this work, we address these challenges using the multifunctional montmorillonite nanosheet-based (MMT-based) drug nanoplatform, which involves the antibiotic 5-fluorocytosine (5-FC), antibacterial metal copper ions, and quaternized chitosan (QCS).
31665888	3	40	theme	Composite	643:651	arg1	QCS/MMT/5-FCCu					662:675	Composite material QCS/MMT/5-FCCu	643:675	Composite material QCS/MMT/5-FCCu	643:675	Composite material QCS/MMT/5-FCCu can can strongly inhibit Staphylococcus aureus (a typical Gram-positive bacterium), Escherichia coli (a typical Gram-negative bacterium), and Candida albicans (a fungus) because 5-FC coordinates with copper ions in situ and due to the deposition of QCS.
31665888	4	41	theme	release	1089:1095	arg1	inhibition					1097:1106	long-acting sustained release inhibition	1067:1106	long-acting sustained release inhibition	1067:1106	The subsequent drug release behavior of 5-FCCu was studied, and the results show an initial high concentration kills microorganisms and long-acting sustained release inhibition.
31665888	1	42	theme	practical	252:260	arg1	application					262:272	their practical application	246:272	their practical application	246:272	Conventional drug delivery systems for natural clay materials still face critical challenges in their practical application, including multiple bacterial infections, combined infection of bacteria and fungi, and low sterilization efficiency.
31665888	0	43	theme	Antimicrobial	53:65	arg1	Complexation					105:116	Chitosan-Coated Montmorillonite Interior Antimicrobial Metal-Antibiotic in Situ Coordination Complexation	12:116	Chitosan-Coated Montmorillonite Interior Antimicrobial Metal-Antibiotic in Situ Coordination Complexation for Mixed Infections of Wounds	12:147	Quaternized Chitosan-Coated Montmorillonite Interior Antimicrobial Metal-Antibiotic in Situ Coordination Complexation for Mixed Infections of Wounds.
31665888	5	44	theme	healing	1198:1204	arg1	promotion					1179:1187	the promotion	1175:1187	the promotion of wound healing and excellent biocompatibility	1175:1235	Moreover, in vivo wound experiments and toxicity experiments show the promotion of wound healing and excellent biocompatibility.
31665888	2	45	theme	copper	598:603	arg1	ions					605:608	antibacterial metal copper ions	578:608	antibacterial metal copper ions	578:608	In this work, we address these challenges using the multifunctional montmorillonite nanosheet-based (MMT-based) drug nanoplatform, which involves the antibiotic 5-fluorocytosine (5-FC), antibacterial metal copper ions, and quaternized chitosan (QCS).
31665888	5	46	theme	wound	1127:1131	arg1	experiments					1133:1143	in vivo wound experiments	1119:1143	in vivo wound experiments	1119:1143	Moreover, in vivo wound experiments and toxicity experiments show the promotion of wound healing and excellent biocompatibility.
31665888	0	47	dep	in	84:85	arg1	Situ					87:90	Situ	87:90	Situ	87:90	Quaternized Chitosan-Coated Montmorillonite Interior Antimicrobial Metal-Antibiotic in Situ Coordination Complexation for Mixed Infections of Wounds.
31665888	3	48	with	coordinates	860:870	arg1	ions					884:887	copper ions	877:887	copper ions	877:887	Composite material QCS/MMT/5-FCCu can can strongly inhibit Staphylococcus aureus (a typical Gram-positive bacterium), Escherichia coli (a typical Gram-negative bacterium), and Candida albicans (a fungus) because 5-FC coordinates with copper ions in situ and due to the deposition of QCS.
31665888	2	49	theme	multifunctional	444:458	arg1	nanoplatform					509:520	the multifunctional montmorillonite nanosheet-based (MMT-based) drug nanoplatform	440:520	the multifunctional montmorillonite nanosheet-based (MMT-based) drug nanoplatform	440:520	In this work, we address these challenges using the multifunctional montmorillonite nanosheet-based (MMT-based) drug nanoplatform, which involves the antibiotic 5-fluorocytosine (5-FC), antibacterial metal copper ions, and quaternized chitosan (QCS).
31665888	4	50	theme	subsequent	935:944	arg1	behavior					959:966	The subsequent drug release behavior	931:966	The subsequent drug release behavior of 5-FCCu	931:976	The subsequent drug release behavior of 5-FCCu was studied, and the results show an initial high concentration kills microorganisms and long-acting sustained release inhibition.
31665888	6	51	used	used	1342:1345	arg2	platform					1326:1333	the MMT-based smart platform	1306:1333	the MMT-based smart platform	1306:1333	As a demonstration of the utility of the latter, we have shown that the MMT-based smart platform can be used for the treatment of mixed infections of wounds.
31665888	6	52	theme	latter	1279:1284	arg1	utility					1264:1270	the utility	1260:1270	the utility of the latter	1260:1284	As a demonstration of the utility of the latter, we have shown that the MMT-based smart platform can be used for the treatment of mixed infections of wounds.
31665888	4	53	dep	show	1007:1010	arg1	kills					1042:1046	kills	1042:1046	show an initial high concentration kills microorganisms and long-acting sustained release inhibition	1007:1106	The subsequent drug release behavior of 5-FCCu was studied, and the results show an initial high concentration kills microorganisms and long-acting sustained release inhibition.
31665888	1	54	theme	Conventional	150:161	arg1	systems					177:183	Conventional drug delivery systems	150:183	Conventional drug delivery systems for natural clay materials	150:210	Conventional drug delivery systems for natural clay materials still face critical challenges in their practical application, including multiple bacterial infections, combined infection of bacteria and fungi, and low sterilization efficiency.
31665888	0	55	theme	in	84:85	arg1	Complexation					105:116	Chitosan-Coated Montmorillonite Interior Antimicrobial Metal-Antibiotic in Situ Coordination Complexation	12:116	Chitosan-Coated Montmorillonite Interior Antimicrobial Metal-Antibiotic in Situ Coordination Complexation for Mixed Infections of Wounds	12:147	Quaternized Chitosan-Coated Montmorillonite Interior Antimicrobial Metal-Antibiotic in Situ Coordination Complexation for Mixed Infections of Wounds.
31665888	1	56	theme	low	362:364	arg1	efficiency					380:389	low sterilization efficiency	362:389	low sterilization efficiency	362:389	Conventional drug delivery systems for natural clay materials still face critical challenges in their practical application, including multiple bacterial infections, combined infection of bacteria and fungi, and low sterilization efficiency.
31665888	3	57	theme	Gram-positive	735:747	arg1	aureus					717:722	Staphylococcus aureus	702:722	Staphylococcus aureus (a typical Gram-positive bacterium)	702:758	Composite material QCS/MMT/5-FCCu can can strongly inhibit Staphylococcus aureus (a typical Gram-positive bacterium), Escherichia coli (a typical Gram-negative bacterium), and Candida albicans (a fungus) because 5-FC coordinates with copper ions in situ and due to the deposition of QCS.
31665888	3	57	theme	Gram-positive	735:747	arg1	bacterium					749:757	a typical Gram-positive bacterium	725:757	a typical Gram-positive bacterium	725:757	Composite material QCS/MMT/5-FCCu can can strongly inhibit Staphylococcus aureus (a typical Gram-positive bacterium), Escherichia coli (a typical Gram-negative bacterium), and Candida albicans (a fungus) because 5-FC coordinates with copper ions in situ and due to the deposition of QCS.
31665888	1	58	theme	drug	163:166	arg1	systems					177:183	Conventional drug delivery systems	150:183	Conventional drug delivery systems for natural clay materials	150:210	Conventional drug delivery systems for natural clay materials still face critical challenges in their practical application, including multiple bacterial infections, combined infection of bacteria and fungi, and low sterilization efficiency.
31665888	1	59	theme	sterilization	366:378	arg1	efficiency					380:389	low sterilization efficiency	362:389	low sterilization efficiency	362:389	Conventional drug delivery systems for natural clay materials still face critical challenges in their practical application, including multiple bacterial infections, combined infection of bacteria and fungi, and low sterilization efficiency.
31665888	4	60	theme	release	951:957	arg1	behavior					959:966	The subsequent drug release behavior	931:966	The subsequent drug release behavior of 5-FCCu	931:976	The subsequent drug release behavior of 5-FCCu was studied, and the results show an initial high concentration kills microorganisms and long-acting sustained release inhibition.
31665888	4	61	theme	sustained	1079:1087	arg1	release					1089:1095	long-acting sustained release	1067:1095	long-acting sustained release inhibition	1067:1106	The subsequent drug release behavior of 5-FCCu was studied, and the results show an initial high concentration kills microorganisms and long-acting sustained release inhibition.
31665888	3	62	theme	material	653:660	arg1	QCS/MMT/5-FCCu					662:675	Composite material QCS/MMT/5-FCCu	643:675	Composite material QCS/MMT/5-FCCu	643:675	Composite material QCS/MMT/5-FCCu can can strongly inhibit Staphylococcus aureus (a typical Gram-positive bacterium), Escherichia coli (a typical Gram-negative bacterium), and Candida albicans (a fungus) because 5-FC coordinates with copper ions in situ and due to the deposition of QCS.
31665888	1	63	theme	delivery	168:175	arg1	systems					177:183	Conventional drug delivery systems	150:183	Conventional drug delivery systems for natural clay materials	150:210	Conventional drug delivery systems for natural clay materials still face critical challenges in their practical application, including multiple bacterial infections, combined infection of bacteria and fungi, and low sterilization efficiency.
31665888	3	64	dep	inhibit	694:700	arg1	can					677:679	can	677:679	can	677:679	Composite material QCS/MMT/5-FCCu can can strongly inhibit Staphylococcus aureus (a typical Gram-positive bacterium), Escherichia coli (a typical Gram-negative bacterium), and Candida albicans (a fungus) because 5-FC coordinates with copper ions in situ and due to the deposition of QCS.
31665888	3	65	theme	 because	846:853	arg1	coordinates					860:870	Staphylococcus aureus (a typical Gram-positive bacterium), Escherichia coli (a typical Gram-negative bacterium), and Candida albicans (a fungus) because 5-FC coordinates	702:870	Staphylococcus aureus (a typical Gram-positive bacterium), Escherichia coli (a typical Gram-negative bacterium), and Candida albicans (a fungus) because 5-FC coordinates with copper ions	702:887	Composite material QCS/MMT/5-FCCu can can strongly inhibit Staphylococcus aureus (a typical Gram-positive bacterium), Escherichia coli (a typical Gram-negative bacterium), and Candida albicans (a fungus) because 5-FC coordinates with copper ions in situ and due to the deposition of QCS.
31665888	2	66	theme	quaternized	615:625	arg1	chitosan					627:634	quaternized chitosan	615:634	quaternized chitosan (QCS)	615:640	In this work, we address these challenges using the multifunctional montmorillonite nanosheet-based (MMT-based) drug nanoplatform, which involves the antibiotic 5-fluorocytosine (5-FC), antibacterial metal copper ions, and quaternized chitosan (QCS).
31665888	2	66	theme	quaternized	615:625	arg1	QCS					637:639	QCS	637:639	QCS	637:639	In this work, we address these challenges using the multifunctional montmorillonite nanosheet-based (MMT-based) drug nanoplatform, which involves the antibiotic 5-fluorocytosine (5-FC), antibacterial metal copper ions, and quaternized chitosan (QCS).
31665888	5	67	dep	in	1119:1120	arg1	vivo					1122:1125	vivo	1122:1125	vivo	1122:1125	Moreover, in vivo wound experiments and toxicity experiments show the promotion of wound healing and excellent biocompatibility.
31665888	6	68	theme	utility	1264:1270	arg1	demonstration					1243:1255	a demonstration	1241:1255	a demonstration of the utility of the latter	1241:1284	As a demonstration of the utility of the latter, we have shown that the MMT-based smart platform can be used for the treatment of mixed infections of wounds.
31665888	3	69	theme	5-FC	855:858	arg1	coordinates					860:870	Staphylococcus aureus (a typical Gram-positive bacterium), Escherichia coli (a typical Gram-negative bacterium), and Candida albicans (a fungus) because 5-FC coordinates	702:870	Staphylococcus aureus (a typical Gram-positive bacterium), Escherichia coli (a typical Gram-negative bacterium), and Candida albicans (a fungus) because 5-FC coordinates with copper ions	702:887	Composite material QCS/MMT/5-FCCu can can strongly inhibit Staphylococcus aureus (a typical Gram-positive bacterium), Escherichia coli (a typical Gram-negative bacterium), and Candida albicans (a fungus) because 5-FC coordinates with copper ions in situ and due to the deposition of QCS.
31665888	3	70	theme	typical	727:733	arg1	aureus					717:722	Staphylococcus aureus	702:722	Staphylococcus aureus (a typical Gram-positive bacterium)	702:758	Composite material QCS/MMT/5-FCCu can can strongly inhibit Staphylococcus aureus (a typical Gram-positive bacterium), Escherichia coli (a typical Gram-negative bacterium), and Candida albicans (a fungus) because 5-FC coordinates with copper ions in situ and due to the deposition of QCS.
31665888	3	70	theme	typical	727:733	arg1	bacterium					749:757	a typical Gram-positive bacterium	725:757	a typical Gram-positive bacterium	725:757	Composite material QCS/MMT/5-FCCu can can strongly inhibit Staphylococcus aureus (a typical Gram-positive bacterium), Escherichia coli (a typical Gram-negative bacterium), and Candida albicans (a fungus) because 5-FC coordinates with copper ions in situ and due to the deposition of QCS.
31665888	3	71	theme	situ	892:895	arg1	deposition					912:921	the deposition	908:921	situ and due to the deposition of QCS	892:928	Composite material QCS/MMT/5-FCCu can can strongly inhibit Staphylococcus aureus (a typical Gram-positive bacterium), Escherichia coli (a typical Gram-negative bacterium), and Candida albicans (a fungus) because 5-FC coordinates with copper ions in situ and due to the deposition of QCS.
31665888	1	72	theme	multiple	285:292	arg1	infections					304:313	multiple bacterial infections	285:313	multiple bacterial infections	285:313	Conventional drug delivery systems for natural clay materials still face critical challenges in their practical application, including multiple bacterial infections, combined infection of bacteria and fungi, and low sterilization efficiency.
31665888	0	73	theme	Coordination	92:103	arg1	Complexation					105:116	Chitosan-Coated Montmorillonite Interior Antimicrobial Metal-Antibiotic in Situ Coordination Complexation	12:116	Chitosan-Coated Montmorillonite Interior Antimicrobial Metal-Antibiotic in Situ Coordination Complexation for Mixed Infections of Wounds	12:147	Quaternized Chitosan-Coated Montmorillonite Interior Antimicrobial Metal-Antibiotic in Situ Coordination Complexation for Mixed Infections of Wounds.
31665888	4	74	theme	initial	1015:1021	arg1	concentration					1028:1040	an initial high concentration	1012:1040	an initial high concentration	1012:1040	The subsequent drug release behavior of 5-FCCu was studied, and the results show an initial high concentration kills microorganisms and long-acting sustained release inhibition.
31665888	2	75	theme	MMT-based	493:501	arg1	nanoplatform					509:520	the multifunctional montmorillonite nanosheet-based (MMT-based) drug nanoplatform	440:520	the multifunctional montmorillonite nanosheet-based (MMT-based) drug nanoplatform	440:520	In this work, we address these challenges using the multifunctional montmorillonite nanosheet-based (MMT-based) drug nanoplatform, which involves the antibiotic 5-fluorocytosine (5-FC), antibacterial metal copper ions, and quaternized chitosan (QCS).
31665888	1	76	theme	natural	189:195	arg1	materials					202:210	natural clay materials	189:210	natural clay materials	189:210	Conventional drug delivery systems for natural clay materials still face critical challenges in their practical application, including multiple bacterial infections, combined infection of bacteria and fungi, and low sterilization efficiency.
31665888	3	77	theme	QCS	926:928	arg1	deposition					912:921	the deposition	908:921	situ and due to the deposition of QCS	892:928	Composite material QCS/MMT/5-FCCu can can strongly inhibit Staphylococcus aureus (a typical Gram-positive bacterium), Escherichia coli (a typical Gram-negative bacterium), and Candida albicans (a fungus) because 5-FC coordinates with copper ions in situ and due to the deposition of QCS.
31665888	1	78	theme	bacterial	294:302	arg1	infections					304:313	multiple bacterial infections	285:313	multiple bacterial infections	285:313	Conventional drug delivery systems for natural clay materials still face critical challenges in their practical application, including multiple bacterial infections, combined infection of bacteria and fungi, and low sterilization efficiency.
31726126	6	0	theme	antibacterial	708:720	arg1	better					754:759	better	754:759	better	754:759	Further, the antibacterial activity of nZnO/CS was notably better than either of CS and nZnO individually.
31726126	6	0	theme	antibacterial	708:720	arg1	activity					722:729	the antibacterial activity	704:729	the antibacterial activity of nZnO/CS	704:740	Further, the antibacterial activity of nZnO/CS was notably better than either of CS and nZnO individually.
31726126	8	1	theme	alternate-feed	887:900	arg1	drying					908:913	alternate-feed spray drying	887:913	alternate-feed spray drying	887:913	In a word, alternate-feed spray drying presents a novel and cost-efficient method to fabricate multifunctional nZnO/CS microspheres with enhanced biosafety.
31726126	4	2	theme	embedded	573:580	arg1	phase					552:556	the hexagonal phase	538:556	the hexagonal phase	538:556	It was showed that nZnO remained the hexagonal phase, dispersed and embedded into CS microspheres.
31726126	0	3	theme	UV-shielding	99:110	arg1	photodegradation					120:135	antibacterial, UV-shielding and dye photodegradation	84:135	antibacterial, UV-shielding and dye photodegradation	84:135	Preparation of biocompatible nano-ZnO/chitosan microspheres with multi-functions of antibacterial, UV-shielding and dye photodegradation.
31726126	5	4	theme	hemolysis	645:653	arg1	results					617:623	the results	613:623	the results of cytotoxicity and hemolysis test	613:658	Based on the results of cytotoxicity and hemolysis test, biosafety was improved obviously.
31726126	0	5	theme	photodegradation	120:135	arg1	multi-functions					65:79	multi-functions	65:79	multi-functions of antibacterial, UV-shielding and dye photodegradation	65:135	Preparation of biocompatible nano-ZnO/chitosan microspheres with multi-functions of antibacterial, UV-shielding and dye photodegradation.
31726126	8	6	theme	nZnO/CS	987:993	arg1	microspheres					995:1006	multifunctional nZnO/CS microspheres	971:1006	multifunctional nZnO/CS microspheres	971:1006	In a word, alternate-feed spray drying presents a novel and cost-efficient method to fabricate multifunctional nZnO/CS microspheres with enhanced biosafety.
31726126	4	7	theme	CS	587:588	arg1	microspheres					590:601	CS microspheres	587:601	CS microspheres	587:601	It was showed that nZnO remained the hexagonal phase, dispersed and embedded into CS microspheres.
31726126	0	8	theme	dye	116:118	arg1	photodegradation					120:135	antibacterial, UV-shielding and dye photodegradation	84:135	antibacterial, UV-shielding and dye photodegradation	84:135	Preparation of biocompatible nano-ZnO/chitosan microspheres with multi-functions of antibacterial, UV-shielding and dye photodegradation.
31726126	8	9	theme	enhanced	1013:1020	arg1	biosafety					1022:1030	enhanced biosafety	1013:1030	enhanced biosafety	1013:1030	In a word, alternate-feed spray drying presents a novel and cost-efficient method to fabricate multifunctional nZnO/CS microspheres with enhanced biosafety.
31726126	1	10	used	used	170:173	arg2	Nano-ZnO					138:145	Nano-ZnO	138:145	Nano-ZnO (nZnO)	138:152	Nano-ZnO (nZnO) has been widely used as antibacterial, UV-shielding and photocatalysis materials, but limited by its contentious biosafety.
31726126	1	10	used	used	170:173	arg2	antibacterial					178:190	antibacterial	178:190	antibacterial	178:190	Nano-ZnO (nZnO) has been widely used as antibacterial, UV-shielding and photocatalysis materials, but limited by its contentious biosafety.
31726126	1	10	used	used	170:173	arg2	photocatalysis					210:223	photocatalysis	210:223	photocatalysis	210:223	Nano-ZnO (nZnO) has been widely used as antibacterial, UV-shielding and photocatalysis materials, but limited by its contentious biosafety.
31726126	1	10	used	used	170:173	arg2	nZnO					148:151	nZnO	148:151	nZnO	148:151	Nano-ZnO (nZnO) has been widely used as antibacterial, UV-shielding and photocatalysis materials, but limited by its contentious biosafety.
31726126	1	10	used	used	170:173	arg2	UV-shielding					193:204	UV-shielding	193:204	UV-shielding	193:204	Nano-ZnO (nZnO) has been widely used as antibacterial, UV-shielding and photocatalysis materials, but limited by its contentious biosafety.
31726126	3	11	theme	alternate-feed	420:433	arg1	drying					441:446	alternate-feed spray drying	420:446	alternate-feed spray drying	420:446	To solve the problem that nZnO reacts with the CS acid solution, alternate-feed spray drying was adopted to fabricate nZnO/CS composite microspheres.
31726126	3	12	theme	spray	435:439	arg1	drying					441:446	alternate-feed spray drying	420:446	alternate-feed spray drying	420:446	To solve the problem that nZnO reacts with the CS acid solution, alternate-feed spray drying was adopted to fabricate nZnO/CS composite microspheres.
31726126	8	13	attach	presents	915:922	arg1	word					881:884	a word	879:884	a word	879:884	In a word, alternate-feed spray drying presents a novel and cost-efficient method to fabricate multifunctional nZnO/CS microspheres with enhanced biosafety.
31726126	8	13	attach	presents	915:922	arg2	drying					908:913	alternate-feed spray drying	887:913	alternate-feed spray drying	887:913	In a word, alternate-feed spray drying presents a novel and cost-efficient method to fabricate multifunctional nZnO/CS microspheres with enhanced biosafety.
31726126	5	14	theme	cytotoxicity	628:639	arg1	results					617:623	the results	613:623	the results of cytotoxicity and hemolysis test	613:658	Based on the results of cytotoxicity and hemolysis test, biosafety was improved obviously.
31726126	7	15	theme	photocatalytic	828:841	arg1	test					855:858	photocatalytic degradation test	828:858	photocatalytic degradation test	828:858	UV-shielding property and photocatalytic degradation test were estimated.
31726126	0	16	theme	nano-ZnO/chitosan	29:45	arg1	microspheres					47:58	biocompatible nano-ZnO/chitosan microspheres	15:58	biocompatible nano-ZnO/chitosan microspheres	15:58	Preparation of biocompatible nano-ZnO/chitosan microspheres with multi-functions of antibacterial, UV-shielding and dye photodegradation.
31726126	4	17	theme	hexagonal	542:550	arg1	phase					552:556	the hexagonal phase	538:556	the hexagonal phase	538:556	It was showed that nZnO remained the hexagonal phase, dispersed and embedded into CS microspheres.
31726126	5	18	dep	cytotoxicity	628:639	arg1	test					655:658	test	655:658	test	655:658	Based on the results of cytotoxicity and hemolysis test, biosafety was improved obviously.
31726126	7	19	theme	degradation	843:853	arg1	test					855:858	photocatalytic degradation test	828:858	photocatalytic degradation test	828:858	UV-shielding property and photocatalytic degradation test were estimated.
31726126	0	20	theme	biocompatible	15:27	arg1	microspheres					47:58	biocompatible nano-ZnO/chitosan microspheres	15:58	biocompatible nano-ZnO/chitosan microspheres	15:58	Preparation of biocompatible nano-ZnO/chitosan microspheres with multi-functions of antibacterial, UV-shielding and dye photodegradation.
31726126	4	21	theme	dispersed	559:567	arg1	phase					552:556	the hexagonal phase	538:556	the hexagonal phase	538:556	It was showed that nZnO remained the hexagonal phase, dispersed and embedded into CS microspheres.
31726126	8	22	theme	spray	902:906	arg1	drying					908:913	alternate-feed spray drying	887:913	alternate-feed spray drying	887:913	In a word, alternate-feed spray drying presents a novel and cost-efficient method to fabricate multifunctional nZnO/CS microspheres with enhanced biosafety.
31726126	3	23	theme	CS	402:403	arg1	solution					410:417	the CS acid solution	398:417	the CS acid solution	398:417	To solve the problem that nZnO reacts with the CS acid solution, alternate-feed spray drying was adopted to fabricate nZnO/CS composite microspheres.
31726126	0	24	theme	microspheres	47:58	arg1	Preparation					0:10	Preparation	0:10	Preparation of biocompatible nano-ZnO/chitosan microspheres with multi-functions of antibacterial, UV-shielding and dye photodegradation.	0:136	Preparation of biocompatible nano-ZnO/chitosan microspheres with multi-functions of antibacterial, UV-shielding and dye photodegradation.
31726126	3	25	theme	acid	405:408	arg1	solution					410:417	the CS acid solution	398:417	the CS acid solution	398:417	To solve the problem that nZnO reacts with the CS acid solution, alternate-feed spray drying was adopted to fabricate nZnO/CS composite microspheres.
31726126	8	26	theme	novel	926:930	arg1	method					951:956	a novel and cost-efficient method	924:956	a novel and cost-efficient method to fabricate multifunctional nZnO/CS microspheres with enhanced biosafety	924:1030	In a word, alternate-feed spray drying presents a novel and cost-efficient method to fabricate multifunctional nZnO/CS microspheres with enhanced biosafety.
31726126	7	27	theme	UV-shielding	802:813	arg1	property					815:822	UV-shielding property	802:822	UV-shielding property	802:822	UV-shielding property and photocatalytic degradation test were estimated.
31726126	1	28	dep	antibacterial	178:190	arg1	materials					225:233	materials	225:233	materials	225:233	Nano-ZnO (nZnO) has been widely used as antibacterial, UV-shielding and photocatalysis materials, but limited by its contentious biosafety.
31726126	3	29	theme	composite	481:489	arg1	microspheres					491:502	nZnO/CS composite microspheres	473:502	nZnO/CS composite microspheres	473:502	To solve the problem that nZnO reacts with the CS acid solution, alternate-feed spray drying was adopted to fabricate nZnO/CS composite microspheres.
31726126	8	30	theme	cost-efficient	936:949	arg1	method					951:956	a novel and cost-efficient method	924:956	a novel and cost-efficient method to fabricate multifunctional nZnO/CS microspheres with enhanced biosafety	924:1030	In a word, alternate-feed spray drying presents a novel and cost-efficient method to fabricate multifunctional nZnO/CS microspheres with enhanced biosafety.
31726126	0	31	with	Preparation	0:10	arg1	multi-functions					65:79	multi-functions	65:79	multi-functions of antibacterial, UV-shielding and dye photodegradation	65:135	Preparation of biocompatible nano-ZnO/chitosan microspheres with multi-functions of antibacterial, UV-shielding and dye photodegradation.
31726126	6	32	theme	nZnO/CS	734:740	arg1	better					754:759	better	754:759	better	754:759	Further, the antibacterial activity of nZnO/CS was notably better than either of CS and nZnO individually.
31726126	6	32	theme	nZnO/CS	734:740	arg1	activity					722:729	the antibacterial activity	704:729	the antibacterial activity of nZnO/CS	704:740	Further, the antibacterial activity of nZnO/CS was notably better than either of CS and nZnO individually.
31726126	3	33	theme	nZnO/CS	473:479	arg1	microspheres					491:502	nZnO/CS composite microspheres	473:502	nZnO/CS composite microspheres	473:502	To solve the problem that nZnO reacts with the CS acid solution, alternate-feed spray drying was adopted to fabricate nZnO/CS composite microspheres.
31726126	0	34	theme	antibacterial	84:96	arg1	photodegradation					120:135	antibacterial, UV-shielding and dye photodegradation	84:135	antibacterial, UV-shielding and dye photodegradation	84:135	Preparation of biocompatible nano-ZnO/chitosan microspheres with multi-functions of antibacterial, UV-shielding and dye photodegradation.
31726126	8	35	theme	multifunctional	971:985	arg1	microspheres					995:1006	multifunctional nZnO/CS microspheres	971:1006	multifunctional nZnO/CS microspheres	971:1006	In a word, alternate-feed spray drying presents a novel and cost-efficient method to fabricate multifunctional nZnO/CS microspheres with enhanced biosafety.
31726126	1	36	theme	contentious	255:265	arg1	biosafety					267:275	its contentious biosafety	251:275	its contentious biosafety	251:275	Nano-ZnO (nZnO) has been widely used as antibacterial, UV-shielding and photocatalysis materials, but limited by its contentious biosafety.
30236870	0	0	from	effect	29:34	arg1	colitis					92:98	DSS-induced colitis	80:98	DSS-induced colitis in mice	80:106	Intestinal anti-inflammatory effect of the probiotic Saccharomyces boulardii in DSS-induced colitis in mice: Impact on microRNAs expression and gut microbiota composition.
30236870	11	1	from	dysbiosis	1834:1842	arg1	lumen					1862:1866	the intestinal lumen	1847:1866	the intestinal lumen	1847:1866	Saccharomyces boulardii exerted intestinal anti-inflammatory effects in DSS-mouse colitis, through the modulation in the immune response, involving modification of altered miRNA expression, being associated to the improvement of the inflammation-associated dysbiosis in the intestinal lumen, which could be of great interest to control the complex pathogenesis of IBD.
30236870	6	2	theme	colonic	916:922	arg1	specimens					924:932	the colonic specimens	912:932	the colonic specimens	912:932	After sacrifice, the colonic specimens were evaluated by determining the expression of inflammatory markers and micro-RNAs by qRT-PCR.
30236870	2	3	theme	Saccharomyces	533:545	arg1	boulardii					547:555	the probiotic Saccharomyces boulardii	519:555	the probiotic Saccharomyces boulardii	519:555	Therefore, our aim was to correlate the intestinal anti-inflammatory activity of the probiotic Saccharomyces boulardii in the dextran sodium sulphate (DSS) model of mouse colitis and the changes induced in miRNA expression and gut microbiota populations.
30236870	11	4	theme	inflammation-associated	1810:1832	arg1	dysbiosis					1834:1842	the inflammation-associated dysbiosis	1806:1842	the inflammation-associated dysbiosis in the intestinal lumen, which could be of great interest to control the complex pathogenesis of IBD	1806:1943	Saccharomyces boulardii exerted intestinal anti-inflammatory effects in DSS-mouse colitis, through the modulation in the immune response, involving modification of altered miRNA expression, being associated to the improvement of the inflammation-associated dysbiosis in the intestinal lumen, which could be of great interest to control the complex pathogenesis of IBD.
30236870	2	5	theme	DSS	589:591	arg1	model					594:598	the dextran sodium sulphate (DSS) model	560:598	the dextran sodium sulphate (DSS) model of mouse colitis	560:615	Therefore, our aim was to correlate the intestinal anti-inflammatory activity of the probiotic Saccharomyces boulardii in the dextran sodium sulphate (DSS) model of mouse colitis and the changes induced in miRNA expression and gut microbiota populations.
30236870	1	6	theme	gut	410:412	arg1	composition					425:435	the gut microbiota composition	406:435	the gut microbiota composition	406:435	The beneficial effects exerted by probiotics in inflammatory bowel disease (IBD) are well known, although their exact mechanisms have not been fully elucidated, and only few studies have focused on their impact on selected miRNAs and the gut microbiota composition.
30236870	11	7	from	effects	1638:1644	arg1	colitis					1659:1665	DSS-mouse colitis	1649:1665	DSS-mouse colitis	1649:1665	Saccharomyces boulardii exerted intestinal anti-inflammatory effects in DSS-mouse colitis, through the modulation in the immune response, involving modification of altered miRNA expression, being associated to the improvement of the inflammation-associated dysbiosis in the intestinal lumen, which could be of great interest to control the complex pathogenesis of IBD.
30236870	1	8	from	impact	376:381	arg1	composition					425:435	the gut microbiota composition	406:435	the gut microbiota composition	406:435	The beneficial effects exerted by probiotics in inflammatory bowel disease (IBD) are well known, although their exact mechanisms have not been fully elucidated, and only few studies have focused on their impact on selected miRNAs and the gut microbiota composition.
30236870	1	8	from	impact	376:381	arg1	miRNAs					395:400	selected miRNAs	386:400	selected miRNAs	386:400	The beneficial effects exerted by probiotics in inflammatory bowel disease (IBD) are well known, although their exact mechanisms have not been fully elucidated, and only few studies have focused on their impact on selected miRNAs and the gut microbiota composition.
30236870	4	9	theme	drinking	818:825	arg1	water					827:831	the drinking water	814:831	the drinking water	814:831	After 2 weeks, the colitis was induced adding DSS to the drinking water.
30236870	1	10	theme	microbiota	414:423	arg1	composition					425:435	the gut microbiota composition	406:435	the gut microbiota composition	406:435	The beneficial effects exerted by probiotics in inflammatory bowel disease (IBD) are well known, although their exact mechanisms have not been fully elucidated, and only few studies have focused on their impact on selected miRNAs and the gut microbiota composition.
30236870	11	11	theme	Saccharomyces	1577:1589	arg1	boulardii					1591:1599	Saccharomyces boulardii	1577:1599	Saccharomyces boulardii	1577:1599	Saccharomyces boulardii exerted intestinal anti-inflammatory effects in DSS-mouse colitis, through the modulation in the immune response, involving modification of altered miRNA expression, being associated to the improvement of the inflammation-associated dysbiosis in the intestinal lumen, which could be of great interest to control the complex pathogenesis of IBD.
30236870	1	12	theme	few	342:344	arg1	studies					346:352	only few studies	337:352	only few studies	337:352	The beneficial effects exerted by probiotics in inflammatory bowel disease (IBD) are well known, although their exact mechanisms have not been fully elucidated, and only few studies have focused on their impact on selected miRNAs and the gut microbiota composition.
30236870	2	13	theme	sulphate	579:586	arg1	model					594:598	the dextran sodium sulphate (DSS) model	560:598	the dextran sodium sulphate (DSS) model of mouse colitis	560:615	Therefore, our aim was to correlate the intestinal anti-inflammatory activity of the probiotic Saccharomyces boulardii in the dextran sodium sulphate (DSS) model of mouse colitis and the changes induced in miRNA expression and gut microbiota populations.
30236870	11	14	theme	miRNA	1749:1753	arg1	expression					1755:1764	altered miRNA expression	1741:1764	altered miRNA expression	1741:1764	Saccharomyces boulardii exerted intestinal anti-inflammatory effects in DSS-mouse colitis, through the modulation in the immune response, involving modification of altered miRNA expression, being associated to the improvement of the inflammation-associated dysbiosis in the intestinal lumen, which could be of great interest to control the complex pathogenesis of IBD.
30236870	10	15	theme	probiotic	1469:1477	arg1	treatment					1479:1487	probiotic treatment	1469:1487	probiotic treatment	1469:1487	Moreover, microbiota characterization showed that probiotic treatment increased bacterial diversity, thus ameliorating the dysbiosis produced by DSS-colitis.
30236870	9	16	theme	inflammatory	1313:1324	arg1	markers					1326:1332	different inflammatory markers	1303:1332	different inflammatory markers	1303:1332	The treatment modified the colonic expression of different inflammatory markers and the epithelial integrity proteins, and induced changes in micro-RNAs expression.
30236870	2	17	theme	anti-inflammatory	489:505	arg1	activity					507:514	the intestinal anti-inflammatory activity	474:514	the intestinal anti-inflammatory activity of the probiotic Saccharomyces boulardii in the dextran sodium sulphate (DSS) model of mouse colitis and the changes induced in miRNA expression and gut microbiota populations	474:690	Therefore, our aim was to correlate the intestinal anti-inflammatory activity of the probiotic Saccharomyces boulardii in the dextran sodium sulphate (DSS) model of mouse colitis and the changes induced in miRNA expression and gut microbiota populations.
30236870	11	18	theme	immune	1698:1703	arg1	response					1705:1712	the immune response	1694:1712	the immune response	1694:1712	Saccharomyces boulardii exerted intestinal anti-inflammatory effects in DSS-mouse colitis, through the modulation in the immune response, involving modification of altered miRNA expression, being associated to the improvement of the inflammation-associated dysbiosis in the intestinal lumen, which could be of great interest to control the complex pathogenesis of IBD.
30236870	2	19	theme	colitis	609:615	arg1	changes					625:631	the changes	621:631	the changes induced in miRNA expression and gut microbiota populations	621:690	Therefore, our aim was to correlate the intestinal anti-inflammatory activity of the probiotic Saccharomyces boulardii in the dextran sodium sulphate (DSS) model of mouse colitis and the changes induced in miRNA expression and gut microbiota populations.
30236870	2	19	theme	colitis	609:615	arg1	model					594:598	the dextran sodium sulphate (DSS) model	560:598	the dextran sodium sulphate (DSS) model of mouse colitis	560:615	Therefore, our aim was to correlate the intestinal anti-inflammatory activity of the probiotic Saccharomyces boulardii in the dextran sodium sulphate (DSS) model of mouse colitis and the changes induced in miRNA expression and gut microbiota populations.
30236870	8	20	theme	DAI	1187:1189	arg1	values					1191:1196	DAI values	1187:1196	DAI values	1187:1196	Probiotic ameliorated the colonic damage induced by DSS, as evidenced by lower DAI values and colonic weight/length compared with untreated mice.
30236870	1	21	theme	beneficial	176:185	arg1	effects					187:193	The beneficial effects	172:193	The beneficial effects exerted by probiotics in inflammatory bowel disease (IBD)	172:251	The beneficial effects exerted by probiotics in inflammatory bowel disease (IBD) are well known, although their exact mechanisms have not been fully elucidated, and only few studies have focused on their impact on selected miRNAs and the gut microbiota composition.
30236870	2	22	theme	intestinal	478:487	arg1	activity					507:514	the intestinal anti-inflammatory activity	474:514	the intestinal anti-inflammatory activity of the probiotic Saccharomyces boulardii in the dextran sodium sulphate (DSS) model of mouse colitis and the changes induced in miRNA expression and gut microbiota populations	474:690	Therefore, our aim was to correlate the intestinal anti-inflammatory activity of the probiotic Saccharomyces boulardii in the dextran sodium sulphate (DSS) model of mouse colitis and the changes induced in miRNA expression and gut microbiota populations.
30236870	2	23	theme	mouse	603:607	arg1	colitis					609:615	mouse colitis	603:615	mouse colitis	603:615	Therefore, our aim was to correlate the intestinal anti-inflammatory activity of the probiotic Saccharomyces boulardii in the dextran sodium sulphate (DSS) model of mouse colitis and the changes induced in miRNA expression and gut microbiota populations.
30236870	9	24	theme	markers	1326:1332	arg1	expression					1289:1298	the colonic expression	1277:1298	the colonic expression of different inflammatory markers and the epithelial integrity proteins	1277:1370	The treatment modified the colonic expression of different inflammatory markers and the epithelial integrity proteins, and induced changes in micro-RNAs expression.
30236870	9	25	theme	micro-RNAs	1396:1405	arg1	expression					1407:1416	micro-RNAs expression	1396:1416	micro-RNAs expression	1396:1416	The treatment modified the colonic expression of different inflammatory markers and the epithelial integrity proteins, and induced changes in micro-RNAs expression.
30236870	0	26	theme	anti-inflammatory	11:27	arg1	effect					29:34	Intestinal anti-inflammatory effect	0:34	Intestinal anti-inflammatory effect of the probiotic Saccharomyces boulardii in DSS-induced colitis in mice: Impact on microRNAs expression and gut microbiota composition.	0:170	Intestinal anti-inflammatory effect of the probiotic Saccharomyces boulardii in DSS-induced colitis in mice: Impact on microRNAs expression and gut microbiota composition.
30236870	11	27	theme	complex	1917:1923	arg1	pathogenesis					1925:1936	the complex pathogenesis	1913:1936	the complex pathogenesis of IBD	1913:1943	Saccharomyces boulardii exerted intestinal anti-inflammatory effects in DSS-mouse colitis, through the modulation in the immune response, involving modification of altered miRNA expression, being associated to the improvement of the inflammation-associated dysbiosis in the intestinal lumen, which could be of great interest to control the complex pathogenesis of IBD.
30236870	11	28	from	modulation	1680:1689	arg1	response					1705:1712	the immune response	1694:1712	the immune response	1694:1712	Saccharomyces boulardii exerted intestinal anti-inflammatory effects in DSS-mouse colitis, through the modulation in the immune response, involving modification of altered miRNA expression, being associated to the improvement of the inflammation-associated dysbiosis in the intestinal lumen, which could be of great interest to control the complex pathogenesis of IBD.
30236870	5	29	theme	Disease	865:871	arg1	Index					882:886	a Disease Activity Index	863:886	a Disease Activity Index (DAI)	863:892	Mice were scored daily using a Disease Activity Index (DAI).
30236870	5	29	theme	Disease	865:871	arg1	DAI					889:891	DAI	889:891	DAI	889:891	Mice were scored daily using a Disease Activity Index (DAI).
30236870	2	30	theme	dextran	564:570	arg1	model					594:598	the dextran sodium sulphate (DSS) model	560:598	the dextran sodium sulphate (DSS) model of mouse colitis	560:615	Therefore, our aim was to correlate the intestinal anti-inflammatory activity of the probiotic Saccharomyces boulardii in the dextran sodium sulphate (DSS) model of mouse colitis and the changes induced in miRNA expression and gut microbiota populations.
30236870	6	31	theme	markers	995:1001	arg1	expression					968:977	the expression	964:977	the expression of inflammatory markers and micro-RNAs by qRT-PCR	964:1027	After sacrifice, the colonic specimens were evaluated by determining the expression of inflammatory markers and micro-RNAs by qRT-PCR.
30236870	0	32	theme	Intestinal	0:9	arg1	effect					29:34	Intestinal anti-inflammatory effect	0:34	Intestinal anti-inflammatory effect of the probiotic Saccharomyces boulardii in DSS-induced colitis in mice: Impact on microRNAs expression and gut microbiota composition.	0:170	Intestinal anti-inflammatory effect of the probiotic Saccharomyces boulardii in DSS-induced colitis in mice: Impact on microRNAs expression and gut microbiota composition.
30236870	11	33	theme	expression	1755:1764	arg1	modification					1725:1736	modification	1725:1736	modification of altered miRNA expression	1725:1764	Saccharomyces boulardii exerted intestinal anti-inflammatory effects in DSS-mouse colitis, through the modulation in the immune response, involving modification of altered miRNA expression, being associated to the improvement of the inflammation-associated dysbiosis in the intestinal lumen, which could be of great interest to control the complex pathogenesis of IBD.
30236870	2	34	theme	miRNA	644:648	arg1	expression					650:659	miRNA expression	644:659	miRNA expression	644:659	Therefore, our aim was to correlate the intestinal anti-inflammatory activity of the probiotic Saccharomyces boulardii in the dextran sodium sulphate (DSS) model of mouse colitis and the changes induced in miRNA expression and gut microbiota populations.
30236870	5	35	theme	Activity	873:880	arg1	Index					882:886	a Disease Activity Index	863:886	a Disease Activity Index (DAI)	863:892	Mice were scored daily using a Disease Activity Index (DAI).
30236870	5	35	theme	Activity	873:880	arg1	DAI					889:891	DAI	889:891	DAI	889:891	Mice were scored daily using a Disease Activity Index (DAI).
30236870	6	36	theme	inflammatory	982:993	arg1	markers					995:1001	inflammatory markers	982:1001	inflammatory markers	982:1001	After sacrifice, the colonic specimens were evaluated by determining the expression of inflammatory markers and micro-RNAs by qRT-PCR.
30236870	2	37	theme	probiotic	523:531	arg1	boulardii					547:555	the probiotic Saccharomyces boulardii	519:555	the probiotic Saccharomyces boulardii	519:555	Therefore, our aim was to correlate the intestinal anti-inflammatory activity of the probiotic Saccharomyces boulardii in the dextran sodium sulphate (DSS) model of mouse colitis and the changes induced in miRNA expression and gut microbiota populations.
30236870	9	38	theme	colonic	1281:1287	arg1	expression					1289:1298	the colonic expression	1277:1298	the colonic expression of different inflammatory markers and the epithelial integrity proteins	1277:1370	The treatment modified the colonic expression of different inflammatory markers and the epithelial integrity proteins, and induced changes in micro-RNAs expression.
30236870	2	39	theme	boulardii	547:555	arg1	activity					507:514	the intestinal anti-inflammatory activity	474:514	the intestinal anti-inflammatory activity of the probiotic Saccharomyces boulardii in the dextran sodium sulphate (DSS) model of mouse colitis and the changes induced in miRNA expression and gut microbiota populations	474:690	Therefore, our aim was to correlate the intestinal anti-inflammatory activity of the probiotic Saccharomyces boulardii in the dextran sodium sulphate (DSS) model of mouse colitis and the changes induced in miRNA expression and gut microbiota populations.
30236870	11	40	from	improvement	1791:1801	arg1	lumen					1862:1866	the intestinal lumen	1847:1866	the intestinal lumen	1847:1866	Saccharomyces boulardii exerted intestinal anti-inflammatory effects in DSS-mouse colitis, through the modulation in the immune response, involving modification of altered miRNA expression, being associated to the improvement of the inflammation-associated dysbiosis in the intestinal lumen, which could be of great interest to control the complex pathogenesis of IBD.
30236870	8	41	dep	lower	1181:1185	arg1	values					1191:1196	DAI values	1187:1196	DAI values	1187:1196	Probiotic ameliorated the colonic damage induced by DSS, as evidenced by lower DAI values and colonic weight/length compared with untreated mice.
30236870	1	42	theme	exact	284:288	arg1	mechanisms					290:299	their exact mechanisms	278:299	their exact mechanisms	278:299	The beneficial effects exerted by probiotics in inflammatory bowel disease (IBD) are well known, although their exact mechanisms have not been fully elucidated, and only few studies have focused on their impact on selected miRNAs and the gut microbiota composition.
30236870	0	43	theme	microRNAs	119:127	arg1	expression					129:138	microRNAs expression	119:138	microRNAs expression	119:138	Intestinal anti-inflammatory effect of the probiotic Saccharomyces boulardii in DSS-induced colitis in mice: Impact on microRNAs expression and gut microbiota composition.
30236870	0	44	theme	probiotic	43:51	arg1	boulardii					67:75	the probiotic Saccharomyces boulardii	39:75	the probiotic Saccharomyces boulardii	39:75	Intestinal anti-inflammatory effect of the probiotic Saccharomyces boulardii in DSS-induced colitis in mice: Impact on microRNAs expression and gut microbiota composition.
30236870	11	45	theme	dysbiosis	1834:1842	arg1	improvement					1791:1801	the improvement	1787:1801	the improvement of the inflammation-associated dysbiosis in the intestinal lumen, which could be of great interest to control the complex pathogenesis of IBD	1787:1943	Saccharomyces boulardii exerted intestinal anti-inflammatory effects in DSS-mouse colitis, through the modulation in the immune response, involving modification of altered miRNA expression, being associated to the improvement of the inflammation-associated dysbiosis in the intestinal lumen, which could be of great interest to control the complex pathogenesis of IBD.
30236870	0	46	theme	gut	144:146	arg1	composition					159:169	gut microbiota composition	144:169	gut microbiota composition	144:169	Intestinal anti-inflammatory effect of the probiotic Saccharomyces boulardii in DSS-induced colitis in mice: Impact on microRNAs expression and gut microbiota composition.
30236870	0	47	from	colitis	92:98	arg1	mice					103:106	mice	103:106	mice	103:106	Intestinal anti-inflammatory effect of the probiotic Saccharomyces boulardii in DSS-induced colitis in mice: Impact on microRNAs expression and gut microbiota composition.
30236870	11	48	theme	altered	1741:1747	arg1	expression					1755:1764	altered miRNA expression	1741:1764	altered miRNA expression	1741:1764	Saccharomyces boulardii exerted intestinal anti-inflammatory effects in DSS-mouse colitis, through the modulation in the immune response, involving modification of altered miRNA expression, being associated to the improvement of the inflammation-associated dysbiosis in the intestinal lumen, which could be of great interest to control the complex pathogenesis of IBD.
30236870	1	49	theme	inflammatory	220:231	arg1	IBD					248:250	IBD	248:250	IBD	248:250	The beneficial effects exerted by probiotics in inflammatory bowel disease (IBD) are well known, although their exact mechanisms have not been fully elucidated, and only few studies have focused on their impact on selected miRNAs and the gut microbiota composition.
30236870	1	49	theme	inflammatory	220:231	arg1	disease					239:245	inflammatory bowel disease	220:245	inflammatory bowel disease (IBD)	220:251	The beneficial effects exerted by probiotics in inflammatory bowel disease (IBD) are well known, although their exact mechanisms have not been fully elucidated, and only few studies have focused on their impact on selected miRNAs and the gut microbiota composition.
30236870	6	50	theme	micro-RNAs	1007:1016	arg1	expression					968:977	the expression	964:977	the expression of inflammatory markers and micro-RNAs by qRT-PCR	964:1027	After sacrifice, the colonic specimens were evaluated by determining the expression of inflammatory markers and micro-RNAs by qRT-PCR.
30236870	9	51	theme	different	1303:1311	arg1	markers					1326:1332	different inflammatory markers	1303:1332	different inflammatory markers	1303:1332	The treatment modified the colonic expression of different inflammatory markers and the epithelial integrity proteins, and induced changes in micro-RNAs expression.
30236870	2	52	theme	microbiota	669:678	arg1	populations					680:690	gut microbiota populations	665:690	gut microbiota populations	665:690	Therefore, our aim was to correlate the intestinal anti-inflammatory activity of the probiotic Saccharomyces boulardii in the dextran sodium sulphate (DSS) model of mouse colitis and the changes induced in miRNA expression and gut microbiota populations.
30236870	1	53	theme	bowel	233:237	arg1	IBD					248:250	IBD	248:250	IBD	248:250	The beneficial effects exerted by probiotics in inflammatory bowel disease (IBD) are well known, although their exact mechanisms have not been fully elucidated, and only few studies have focused on their impact on selected miRNAs and the gut microbiota composition.
30236870	1	53	theme	bowel	233:237	arg1	disease					239:245	inflammatory bowel disease	220:245	inflammatory bowel disease (IBD)	220:251	The beneficial effects exerted by probiotics in inflammatory bowel disease (IBD) are well known, although their exact mechanisms have not been fully elucidated, and only few studies have focused on their impact on selected miRNAs and the gut microbiota composition.
30236870	0	54	theme	boulardii	67:75	arg1	effect					29:34	Intestinal anti-inflammatory effect	0:34	Intestinal anti-inflammatory effect of the probiotic Saccharomyces boulardii in DSS-induced colitis in mice: Impact on microRNAs expression and gut microbiota composition.	0:170	Intestinal anti-inflammatory effect of the probiotic Saccharomyces boulardii in DSS-induced colitis in mice: Impact on microRNAs expression and gut microbiota composition.
30236870	7	55	from	changes	1040:1046	arg1	populations					1062:1072	microbiota populations	1051:1072	microbiota populations	1051:1072	Moreover, changes in microbiota populations were evaluated by pyrosequencing.
30236870	2	56	theme	sodium	572:577	arg1	model					594:598	the dextran sodium sulphate (DSS) model	560:598	the dextran sodium sulphate (DSS) model of mouse colitis	560:615	Therefore, our aim was to correlate the intestinal anti-inflammatory activity of the probiotic Saccharomyces boulardii in the dextran sodium sulphate (DSS) model of mouse colitis and the changes induced in miRNA expression and gut microbiota populations.
30236870	11	57	theme	intestinal	1609:1618	arg1	effects					1638:1644	intestinal anti-inflammatory effects	1609:1644	intestinal anti-inflammatory effects in DSS-mouse colitis	1609:1665	Saccharomyces boulardii exerted intestinal anti-inflammatory effects in DSS-mouse colitis, through the modulation in the immune response, involving modification of altered miRNA expression, being associated to the improvement of the inflammation-associated dysbiosis in the intestinal lumen, which could be of great interest to control the complex pathogenesis of IBD.
30236870	0	58	theme	Saccharomyces	53:65	arg1	boulardii					67:75	the probiotic Saccharomyces boulardii	39:75	the probiotic Saccharomyces boulardii	39:75	Intestinal anti-inflammatory effect of the probiotic Saccharomyces boulardii in DSS-induced colitis in mice: Impact on microRNAs expression and gut microbiota composition.
30236870	3	59	dep	given	707:711	arg1	CFU					727:729	5×109 CFU	721:729	5×109 CFU	721:729	Probiotic was given orally (5×109 CFU) to C57BL/6 mice for 26 days.
30236870	0	60	theme	microbiota	148:157	arg1	composition					159:169	gut microbiota composition	144:169	gut microbiota composition	144:169	Intestinal anti-inflammatory effect of the probiotic Saccharomyces boulardii in DSS-induced colitis in mice: Impact on microRNAs expression and gut microbiota composition.
30236870	1	61	theme	selected	386:393	arg1	miRNAs					395:400	selected miRNAs	386:400	selected miRNAs	386:400	The beneficial effects exerted by probiotics in inflammatory bowel disease (IBD) are well known, although their exact mechanisms have not been fully elucidated, and only few studies have focused on their impact on selected miRNAs and the gut microbiota composition.
30236870	7	62	theme	microbiota	1051:1060	arg1	populations					1062:1072	microbiota populations	1051:1072	microbiota populations	1051:1072	Moreover, changes in microbiota populations were evaluated by pyrosequencing.
30236870	1	63	from	probiotics	206:215	arg1	IBD					248:250	IBD	248:250	IBD	248:250	The beneficial effects exerted by probiotics in inflammatory bowel disease (IBD) are well known, although their exact mechanisms have not been fully elucidated, and only few studies have focused on their impact on selected miRNAs and the gut microbiota composition.
30236870	1	63	from	probiotics	206:215	arg1	disease					239:245	inflammatory bowel disease	220:245	inflammatory bowel disease (IBD)	220:251	The beneficial effects exerted by probiotics in inflammatory bowel disease (IBD) are well known, although their exact mechanisms have not been fully elucidated, and only few studies have focused on their impact on selected miRNAs and the gut microbiota composition.
30236870	11	64	theme	great	1887:1891	arg1	interest					1893:1900	great interest	1887:1900	great interest	1887:1900	Saccharomyces boulardii exerted intestinal anti-inflammatory effects in DSS-mouse colitis, through the modulation in the immune response, involving modification of altered miRNA expression, being associated to the improvement of the inflammation-associated dysbiosis in the intestinal lumen, which could be of great interest to control the complex pathogenesis of IBD.
30236870	10	65	theme	microbiota	1429:1438	arg1	characterization					1440:1455	microbiota characterization	1429:1455	microbiota characterization	1429:1455	Moreover, microbiota characterization showed that probiotic treatment increased bacterial diversity, thus ameliorating the dysbiosis produced by DSS-colitis.
30236870	3	66	theme	C57BL/6	735:741	arg1	mice					743:746	C57BL/6 mice	735:746	C57BL/6 mice	735:746	Probiotic was given orally (5×109 CFU) to C57BL/6 mice for 26 days.
30236870	10	67	theme	bacterial	1499:1507	arg1	diversity					1509:1517	bacterial diversity	1499:1517	bacterial diversity	1499:1517	Moreover, microbiota characterization showed that probiotic treatment increased bacterial diversity, thus ameliorating the dysbiosis produced by DSS-colitis.
30236870	0	68	from	Impact	109:114	arg1	composition					159:169	gut microbiota composition	144:169	gut microbiota composition	144:169	Intestinal anti-inflammatory effect of the probiotic Saccharomyces boulardii in DSS-induced colitis in mice: Impact on microRNAs expression and gut microbiota composition.
30236870	0	68	from	Impact	109:114	arg1	expression					129:138	microRNAs expression	119:138	microRNAs expression	119:138	Intestinal anti-inflammatory effect of the probiotic Saccharomyces boulardii in DSS-induced colitis in mice: Impact on microRNAs expression and gut microbiota composition.
30236870	2	69	theme	gut	665:667	arg1	populations					680:690	gut microbiota populations	665:690	gut microbiota populations	665:690	Therefore, our aim was to correlate the intestinal anti-inflammatory activity of the probiotic Saccharomyces boulardii in the dextran sodium sulphate (DSS) model of mouse colitis and the changes induced in miRNA expression and gut microbiota populations.
30236870	8	70	theme	colonic	1202:1208	arg1	weight/length					1210:1222	colonic weight/length	1202:1222	colonic weight/length	1202:1222	Probiotic ameliorated the colonic damage induced by DSS, as evidenced by lower DAI values and colonic weight/length compared with untreated mice.
30236870	11	71	from	lumen	1862:1866	arg1	improvement					1791:1801	the improvement	1787:1801	the improvement of the inflammation-associated dysbiosis in the intestinal lumen, which could be of great interest to control the complex pathogenesis of IBD	1787:1943	Saccharomyces boulardii exerted intestinal anti-inflammatory effects in DSS-mouse colitis, through the modulation in the immune response, involving modification of altered miRNA expression, being associated to the improvement of the inflammation-associated dysbiosis in the intestinal lumen, which could be of great interest to control the complex pathogenesis of IBD.
30236870	0	72	dep	effect	29:34	arg1	Impact					109:114	Impact	109:114	Intestinal anti-inflammatory effect of the probiotic Saccharomyces boulardii in DSS-induced colitis in mice: Impact on microRNAs expression and gut microbiota composition.	0:170	Intestinal anti-inflammatory effect of the probiotic Saccharomyces boulardii in DSS-induced colitis in mice: Impact on microRNAs expression and gut microbiota composition.
30236870	8	73	theme	untreated	1238:1246	arg1	mice					1248:1251	untreated mice	1238:1251	untreated mice	1238:1251	Probiotic ameliorated the colonic damage induced by DSS, as evidenced by lower DAI values and colonic weight/length compared with untreated mice.
30236870	11	74	theme	intestinal	1851:1860	arg1	lumen					1862:1866	the intestinal lumen	1847:1866	the intestinal lumen	1847:1866	Saccharomyces boulardii exerted intestinal anti-inflammatory effects in DSS-mouse colitis, through the modulation in the immune response, involving modification of altered miRNA expression, being associated to the improvement of the inflammation-associated dysbiosis in the intestinal lumen, which could be of great interest to control the complex pathogenesis of IBD.
30236870	9	75	theme	epithelial	1342:1351	arg1	integrity					1353:1361	the epithelial integrity	1338:1361	the epithelial integrity proteins	1338:1370	The treatment modified the colonic expression of different inflammatory markers and the epithelial integrity proteins, and induced changes in micro-RNAs expression.
30236870	0	76	theme	DSS-induced	80:90	arg1	colitis					92:98	DSS-induced colitis	80:98	DSS-induced colitis in mice	80:106	Intestinal anti-inflammatory effect of the probiotic Saccharomyces boulardii in DSS-induced colitis in mice: Impact on microRNAs expression and gut microbiota composition.
30236870	2	77	from	activity	507:514	arg1	changes					625:631	the changes	621:631	the changes induced in miRNA expression and gut microbiota populations	621:690	Therefore, our aim was to correlate the intestinal anti-inflammatory activity of the probiotic Saccharomyces boulardii in the dextran sodium sulphate (DSS) model of mouse colitis and the changes induced in miRNA expression and gut microbiota populations.
30236870	2	77	from	activity	507:514	arg1	model					594:598	the dextran sodium sulphate (DSS) model	560:598	the dextran sodium sulphate (DSS) model of mouse colitis	560:615	Therefore, our aim was to correlate the intestinal anti-inflammatory activity of the probiotic Saccharomyces boulardii in the dextran sodium sulphate (DSS) model of mouse colitis and the changes induced in miRNA expression and gut microbiota populations.
30236870	11	78	theme	DSS-mouse	1649:1657	arg1	colitis					1659:1665	DSS-mouse colitis	1649:1665	DSS-mouse colitis	1649:1665	Saccharomyces boulardii exerted intestinal anti-inflammatory effects in DSS-mouse colitis, through the modulation in the immune response, involving modification of altered miRNA expression, being associated to the improvement of the inflammation-associated dysbiosis in the intestinal lumen, which could be of great interest to control the complex pathogenesis of IBD.
30236870	11	79	theme	IBD	1941:1943	arg1	pathogenesis					1925:1936	the complex pathogenesis	1913:1936	the complex pathogenesis of IBD	1913:1943	Saccharomyces boulardii exerted intestinal anti-inflammatory effects in DSS-mouse colitis, through the modulation in the immune response, involving modification of altered miRNA expression, being associated to the improvement of the inflammation-associated dysbiosis in the intestinal lumen, which could be of great interest to control the complex pathogenesis of IBD.
30236870	9	80	theme	integrity	1353:1361	arg1	proteins					1363:1370	the epithelial integrity proteins	1338:1370	the epithelial integrity proteins	1338:1370	The treatment modified the colonic expression of different inflammatory markers and the epithelial integrity proteins, and induced changes in micro-RNAs expression.
30236870	9	81	from	changes	1385:1391	arg1	expression					1407:1416	micro-RNAs expression	1396:1416	micro-RNAs expression	1396:1416	The treatment modified the colonic expression of different inflammatory markers and the epithelial integrity proteins, and induced changes in micro-RNAs expression.
30236870	11	82	theme	anti-inflammatory	1620:1636	arg1	effects					1638:1644	intestinal anti-inflammatory effects	1609:1644	intestinal anti-inflammatory effects in DSS-mouse colitis	1609:1665	Saccharomyces boulardii exerted intestinal anti-inflammatory effects in DSS-mouse colitis, through the modulation in the immune response, involving modification of altered miRNA expression, being associated to the improvement of the inflammation-associated dysbiosis in the intestinal lumen, which could be of great interest to control the complex pathogenesis of IBD.
30236870	8	83	theme	colonic	1134:1140	arg1	damage					1142:1147	the colonic damage	1130:1147	the colonic damage induced by DSS	1130:1162	Probiotic ameliorated the colonic damage induced by DSS, as evidenced by lower DAI values and colonic weight/length compared with untreated mice.
30236870	9	84	theme	proteins	1363:1370	arg1	expression					1289:1298	the colonic expression	1277:1298	the colonic expression of different inflammatory markers and the epithelial integrity proteins	1277:1370	The treatment modified the colonic expression of different inflammatory markers and the epithelial integrity proteins, and induced changes in micro-RNAs expression.
29799117	6	0	dep	APPLICATION	1090:1100	arg1	coating					1148:1154	The sodium alginate-tea polyphenols composite coating	1102:1154	PRACTICAL APPLICATION The sodium alginate-tea polyphenols composite coating	1080:1154	PRACTICAL APPLICATION The sodium alginate-tea polyphenols composite coating has strong potential to be used as a new biopreservative for maintaining fish fillet quality.
29799117	1	1	theme	fish	276:279	arg1	products					281:288	fish products	276:288	fish products	276:288	Sodium alginate (SA) and tea polyphenols (TP) are natural preservatives commonly used in the food industry, including the production of fish products.
29799117	6	2	theme	polyphenols	1126:1136	arg1	coating					1148:1154	The sodium alginate-tea polyphenols composite coating	1102:1154	PRACTICAL APPLICATION The sodium alginate-tea polyphenols composite coating	1080:1154	PRACTICAL APPLICATION The sodium alginate-tea polyphenols composite coating has strong potential to be used as a new biopreservative for maintaining fish fillet quality.
29799117	3	3	theme	relative	793:800	arg1	period					785:790	the storage period	773:790	the storage period	773:790	Fish fillet samples treated with SA-TP had significantly lower levels of total volatile basic nitrogen, lipid oxidation, and protein decomposition during the storage period, relative to the remaining treatments.
29799117	3	4	theme	basic	707:711	arg1	nitrogen					713:720	total volatile basic nitrogen	692:720	total volatile basic nitrogen	692:720	Fish fillet samples treated with SA-TP had significantly lower levels of total volatile basic nitrogen, lipid oxidation, and protein decomposition during the storage period, relative to the remaining treatments.
29799117	1	5	theme	natural	190:196	arg1	preservatives					198:210	natural preservatives	190:210	natural preservatives	190:210	Sodium alginate (SA) and tea polyphenols (TP) are natural preservatives commonly used in the food industry, including the production of fish products.
29799117	1	5	theme	natural	190:196	arg1	production					262:271	the production	258:271	the production of fish products	258:288	Sodium alginate (SA) and tea polyphenols (TP) are natural preservatives commonly used in the food industry, including the production of fish products.
29799117	1	5	theme	natural	190:196	arg1	alginate					147:154	Sodium alginate	140:154	Sodium alginate (SA)	140:159	Sodium alginate (SA) and tea polyphenols (TP) are natural preservatives commonly used in the food industry, including the production of fish products.
29799117	1	5	theme	natural	190:196	arg1	polyphenols					169:179	tea polyphenols	165:179	tea polyphenols (TP)	165:184	Sodium alginate (SA) and tea polyphenols (TP) are natural preservatives commonly used in the food industry, including the production of fish products.
29799117	2	6	theme	fillets	398:404	arg1	quality					339:345	the quality	335:345	the quality of fresh Japanese sea bass (Lateolabrax japonicas) fillets	335:404	The effect of SA coating infused with TP on the quality of fresh Japanese sea bass (Lateolabrax japonicas) fillets was evaluated over a 20-day period at 4 °C. SA (1.5%, w/v) or TP (0.5%, w/v) treatment alone, and the SA coating infused with TP (SA-TP) all reduced microbial counts, with the SA-TP providing the greatest effect.
29799117	1	7	theme	products	281:288	arg1	production					262:271	the production	258:271	the production of fish products	258:288	Sodium alginate (SA) and tea polyphenols (TP) are natural preservatives commonly used in the food industry, including the production of fish products.
29799117	6	8	theme	alginate-tea	1113:1124	arg1	coating					1148:1154	The sodium alginate-tea polyphenols composite coating	1102:1154	PRACTICAL APPLICATION The sodium alginate-tea polyphenols composite coating	1080:1154	PRACTICAL APPLICATION The sodium alginate-tea polyphenols composite coating has strong potential to be used as a new biopreservative for maintaining fish fillet quality.
29799117	4	9	theme	sensory	878:884	arg1	rating					894:899	the highest sensory quality rating	866:899	the highest sensory quality rating	866:899	The samples treated with SA-TP had the highest sensory quality rating as well.
29799117	2	10	dep	SA	450:451	arg1	w/v					460:462	w/v	460:462	w/v	460:462	The effect of SA coating infused with TP on the quality of fresh Japanese sea bass (Lateolabrax japonicas) fillets was evaluated over a 20-day period at 4 °C. SA (1.5%, w/v) or TP (0.5%, w/v) treatment alone, and the SA coating infused with TP (SA-TP) all reduced microbial counts, with the SA-TP providing the greatest effect.
29799117	2	10	dep	SA	450:451	arg1	%					457:457	1.5%	454:457	1.5%	454:457	The effect of SA coating infused with TP on the quality of fresh Japanese sea bass (Lateolabrax japonicas) fillets was evaluated over a 20-day period at 4 °C. SA (1.5%, w/v) or TP (0.5%, w/v) treatment alone, and the SA coating infused with TP (SA-TP) all reduced microbial counts, with the SA-TP providing the greatest effect.
29799117	2	11	theme	TP	468:469	arg1	treatment					483:491	4 °C. SA (1.5%, w/v) or TP (0.5%, w/v) treatment	444:491	treatment	483:491	The effect of SA coating infused with TP on the quality of fresh Japanese sea bass (Lateolabrax japonicas) fillets was evaluated over a 20-day period at 4 °C. SA (1.5%, w/v) or TP (0.5%, w/v) treatment alone, and the SA coating infused with TP (SA-TP) all reduced microbial counts, with the SA-TP providing the greatest effect.
29799117	2	11	theme	TP	468:469	arg1	w/v					478:480	0.5%, w/v	472:480	w/v	478:480	The effect of SA coating infused with TP on the quality of fresh Japanese sea bass (Lateolabrax japonicas) fillets was evaluated over a 20-day period at 4 °C. SA (1.5%, w/v) or TP (0.5%, w/v) treatment alone, and the SA coating infused with TP (SA-TP) all reduced microbial counts, with the SA-TP providing the greatest effect.
29799117	3	12	theme	total	692:696	arg1	nitrogen					713:720	total volatile basic nitrogen	692:720	total volatile basic nitrogen	692:720	Fish fillet samples treated with SA-TP had significantly lower levels of total volatile basic nitrogen, lipid oxidation, and protein decomposition during the storage period, relative to the remaining treatments.
29799117	5	13	theme	Japanese	1033:1040	arg1	fillets					1051:1057	Japanese sea bass fillets	1033:1057	Japanese sea bass fillets	1033:1057	Collectively, sodium alginate coating infused with tea polyphenols may represent a promising treatment for preservation of Japanese sea bass fillets during cold storage.
29799117	6	14	theme	new	1193:1195	arg1	biopreservative					1197:1211	a new biopreservative	1191:1211	a new biopreservative for maintaining fish fillet quality	1191:1247	PRACTICAL APPLICATION The sodium alginate-tea polyphenols composite coating has strong potential to be used as a new biopreservative for maintaining fish fillet quality.
29799117	3	15	theme	remaining	809:817	arg1	treatments					819:828	the remaining treatments	805:828	the remaining treatments	805:828	Fish fillet samples treated with SA-TP had significantly lower levels of total volatile basic nitrogen, lipid oxidation, and protein decomposition during the storage period, relative to the remaining treatments.
29799117	3	16	theme	lipid	723:727	arg1	oxidation					729:737	lipid oxidation	723:737	lipid oxidation	723:737	Fish fillet samples treated with SA-TP had significantly lower levels of total volatile basic nitrogen, lipid oxidation, and protein decomposition during the storage period, relative to the remaining treatments.
29799117	5	17	theme	sea	1042:1044	arg1	fillets					1051:1057	Japanese sea bass fillets	1033:1057	Japanese sea bass fillets	1033:1057	Collectively, sodium alginate coating infused with tea polyphenols may represent a promising treatment for preservation of Japanese sea bass fillets during cold storage.
29799117	0	18	theme	Japanese	89:96	arg1	Bass					102:105	Japanese Sea Bass	89:105	Fresh Japanese Sea Bass (Lateolabrax japonicas) Fillets	83:137	Effect of a Sodium Alginate Coating Infused with Tea Polyphenols on the Quality of Fresh Japanese Sea Bass (Lateolabrax japonicas) Fillets.
29799117	0	18	theme	Japanese	89:96	arg1	japonicas					120:128	Lateolabrax japonicas	108:128	Lateolabrax japonicas	108:128	Effect of a Sodium Alginate Coating Infused with Tea Polyphenols on the Quality of Fresh Japanese Sea Bass (Lateolabrax japonicas) Fillets.
29799117	3	19	theme	oxidation	729:737	arg1	levels					682:687	significantly lower levels	662:687	significantly lower levels of total volatile basic nitrogen, lipid oxidation, and protein decomposition	662:764	Fish fillet samples treated with SA-TP had significantly lower levels of total volatile basic nitrogen, lipid oxidation, and protein decomposition during the storage period, relative to the remaining treatments.
29799117	1	20	theme	Sodium	140:145	arg1	preservatives					198:210	natural preservatives	190:210	natural preservatives	190:210	Sodium alginate (SA) and tea polyphenols (TP) are natural preservatives commonly used in the food industry, including the production of fish products.
29799117	1	20	theme	Sodium	140:145	arg1	SA					157:158	SA	157:158	SA	157:158	Sodium alginate (SA) and tea polyphenols (TP) are natural preservatives commonly used in the food industry, including the production of fish products.
29799117	1	20	theme	Sodium	140:145	arg1	alginate					147:154	Sodium alginate	140:154	Sodium alginate (SA)	140:159	Sodium alginate (SA) and tea polyphenols (TP) are natural preservatives commonly used in the food industry, including the production of fish products.
29799117	1	20	theme	Sodium	140:145	arg1	production					262:271	the production	258:271	the production of fish products	258:288	Sodium alginate (SA) and tea polyphenols (TP) are natural preservatives commonly used in the food industry, including the production of fish products.
29799117	1	20	theme	Sodium	140:145	arg1	polyphenols					169:179	tea polyphenols	165:179	tea polyphenols (TP)	165:184	Sodium alginate (SA) and tea polyphenols (TP) are natural preservatives commonly used in the food industry, including the production of fish products.
29799117	0	21	theme	Fresh	83:87	arg1	Fillets					131:137	Fresh Japanese Sea Bass (Lateolabrax japonicas) Fillets	83:137	Fresh Japanese Sea Bass (Lateolabrax japonicas) Fillets	83:137	Effect of a Sodium Alginate Coating Infused with Tea Polyphenols on the Quality of Fresh Japanese Sea Bass (Lateolabrax japonicas) Fillets.
29799117	3	22	contain	had	658:660	arg2	levels					682:687	significantly lower levels	662:687	significantly lower levels of total volatile basic nitrogen, lipid oxidation, and protein decomposition	662:764	Fish fillet samples treated with SA-TP had significantly lower levels of total volatile basic nitrogen, lipid oxidation, and protein decomposition during the storage period, relative to the remaining treatments.
29799117	3	22	contain	had	658:660	arg1	samples					631:637	Fish fillet samples	619:637	Fish fillet samples treated with SA-TP	619:656	Fish fillet samples treated with SA-TP had significantly lower levels of total volatile basic nitrogen, lipid oxidation, and protein decomposition during the storage period, relative to the remaining treatments.
29799117	2	23	from	effect	295:300	arg1	quality					339:345	the quality	335:345	the quality of fresh Japanese sea bass (Lateolabrax japonicas) fillets	335:404	The effect of SA coating infused with TP on the quality of fresh Japanese sea bass (Lateolabrax japonicas) fillets was evaluated over a 20-day period at 4 °C. SA (1.5%, w/v) or TP (0.5%, w/v) treatment alone, and the SA coating infused with TP (SA-TP) all reduced microbial counts, with the SA-TP providing the greatest effect.
29799117	5	24	theme	fillets	1051:1057	arg1	preservation					1017:1028	preservation	1017:1028	preservation of Japanese sea bass fillets during cold storage	1017:1077	Collectively, sodium alginate coating infused with tea polyphenols may represent a promising treatment for preservation of Japanese sea bass fillets during cold storage.
29799117	3	25	theme	nitrogen	713:720	arg1	levels					682:687	significantly lower levels	662:687	significantly lower levels of total volatile basic nitrogen, lipid oxidation, and protein decomposition	662:764	Fish fillet samples treated with SA-TP had significantly lower levels of total volatile basic nitrogen, lipid oxidation, and protein decomposition during the storage period, relative to the remaining treatments.
29799117	2	26	theme	20-day	427:432	arg1	period					434:439	a 20-day period	425:439	a 20-day period	425:439	The effect of SA coating infused with TP on the quality of fresh Japanese sea bass (Lateolabrax japonicas) fillets was evaluated over a 20-day period at 4 °C. SA (1.5%, w/v) or TP (0.5%, w/v) treatment alone, and the SA coating infused with TP (SA-TP) all reduced microbial counts, with the SA-TP providing the greatest effect.
29799117	2	27	theme	bass	369:372	arg1	fillets					398:404	fresh Japanese sea bass (Lateolabrax japonicas) fillets	350:404	fresh Japanese sea bass (Lateolabrax japonicas) fillets	350:404	The effect of SA coating infused with TP on the quality of fresh Japanese sea bass (Lateolabrax japonicas) fillets was evaluated over a 20-day period at 4 °C. SA (1.5%, w/v) or TP (0.5%, w/v) treatment alone, and the SA coating infused with TP (SA-TP) all reduced microbial counts, with the SA-TP providing the greatest effect.
29799117	5	28	theme	sodium	924:929	arg1	coating					940:946	sodium alginate coating	924:946	sodium alginate coating infused with tea polyphenols	924:975	Collectively, sodium alginate coating infused with tea polyphenols may represent a promising treatment for preservation of Japanese sea bass fillets during cold storage.
29799117	2	29	theme	sea	365:367	arg1	bass					369:372	Japanese sea bass	356:372	fresh Japanese sea bass (Lateolabrax japonicas) fillets	350:404	The effect of SA coating infused with TP on the quality of fresh Japanese sea bass (Lateolabrax japonicas) fillets was evaluated over a 20-day period at 4 °C. SA (1.5%, w/v) or TP (0.5%, w/v) treatment alone, and the SA coating infused with TP (SA-TP) all reduced microbial counts, with the SA-TP providing the greatest effect.
29799117	2	29	theme	sea	365:367	arg1	japonicas					387:395	Lateolabrax japonicas	375:395	Lateolabrax japonicas	375:395	The effect of SA coating infused with TP on the quality of fresh Japanese sea bass (Lateolabrax japonicas) fillets was evaluated over a 20-day period at 4 °C. SA (1.5%, w/v) or TP (0.5%, w/v) treatment alone, and the SA coating infused with TP (SA-TP) all reduced microbial counts, with the SA-TP providing the greatest effect.
29799117	2	30	theme	%	475:475	arg1	treatment					483:491	4 °C. SA (1.5%, w/v) or TP (0.5%, w/v) treatment	444:491	treatment	483:491	The effect of SA coating infused with TP on the quality of fresh Japanese sea bass (Lateolabrax japonicas) fillets was evaluated over a 20-day period at 4 °C. SA (1.5%, w/v) or TP (0.5%, w/v) treatment alone, and the SA coating infused with TP (SA-TP) all reduced microbial counts, with the SA-TP providing the greatest effect.
29799117	2	30	theme	%	475:475	arg1	w/v					478:480	0.5%, w/v	472:480	w/v	478:480	The effect of SA coating infused with TP on the quality of fresh Japanese sea bass (Lateolabrax japonicas) fillets was evaluated over a 20-day period at 4 °C. SA (1.5%, w/v) or TP (0.5%, w/v) treatment alone, and the SA coating infused with TP (SA-TP) all reduced microbial counts, with the SA-TP providing the greatest effect.
29799117	2	31	theme	SA	508:509	arg1	coating					511:517	the SA coating	504:517	the SA coating infused with TP (SA-TP)	504:541	The effect of SA coating infused with TP on the quality of fresh Japanese sea bass (Lateolabrax japonicas) fillets was evaluated over a 20-day period at 4 °C. SA (1.5%, w/v) or TP (0.5%, w/v) treatment alone, and the SA coating infused with TP (SA-TP) all reduced microbial counts, with the SA-TP providing the greatest effect.
29799117	2	32	theme	greatest	602:609	arg1	effect					611:616	the greatest effect	598:616	the greatest effect	598:616	The effect of SA coating infused with TP on the quality of fresh Japanese sea bass (Lateolabrax japonicas) fillets was evaluated over a 20-day period at 4 °C. SA (1.5%, w/v) or TP (0.5%, w/v) treatment alone, and the SA coating infused with TP (SA-TP) all reduced microbial counts, with the SA-TP providing the greatest effect.
29799117	2	33	theme	coating	308:314	arg1	effect					295:300	The effect	291:300	The effect of SA coating infused with TP on the quality of fresh Japanese sea bass (Lateolabrax japonicas) fillets	291:404	The effect of SA coating infused with TP on the quality of fresh Japanese sea bass (Lateolabrax japonicas) fillets was evaluated over a 20-day period at 4 °C. SA (1.5%, w/v) or TP (0.5%, w/v) treatment alone, and the SA coating infused with TP (SA-TP) all reduced microbial counts, with the SA-TP providing the greatest effect.
29799117	0	34	theme	Sodium	12:17	arg1	Coating					28:34	a Sodium Alginate Coating	10:34	a Sodium Alginate Coating	10:34	Effect of a Sodium Alginate Coating Infused with Tea Polyphenols on the Quality of Fresh Japanese Sea Bass (Lateolabrax japonicas) Fillets.
29799117	5	35	theme	promising	993:1001	arg1	treatment					1003:1011	a promising treatment	991:1011	a promising treatment for preservation of Japanese sea bass fillets during cold storage	991:1077	Collectively, sodium alginate coating infused with tea polyphenols may represent a promising treatment for preservation of Japanese sea bass fillets during cold storage.
29799117	0	36	theme	Bass	102:105	arg1	Fillets					131:137	Fresh Japanese Sea Bass (Lateolabrax japonicas) Fillets	83:137	Fresh Japanese Sea Bass (Lateolabrax japonicas) Fillets	83:137	Effect of a Sodium Alginate Coating Infused with Tea Polyphenols on the Quality of Fresh Japanese Sea Bass (Lateolabrax japonicas) Fillets.
29799117	3	37	theme	decomposition	752:764	arg1	levels					682:687	significantly lower levels	662:687	significantly lower levels of total volatile basic nitrogen, lipid oxidation, and protein decomposition	662:764	Fish fillet samples treated with SA-TP had significantly lower levels of total volatile basic nitrogen, lipid oxidation, and protein decomposition during the storage period, relative to the remaining treatments.
29799117	2	38	theme	fresh	350:354	arg1	fillets					398:404	fresh Japanese sea bass (Lateolabrax japonicas) fillets	350:404	fresh Japanese sea bass (Lateolabrax japonicas) fillets	350:404	The effect of SA coating infused with TP on the quality of fresh Japanese sea bass (Lateolabrax japonicas) fillets was evaluated over a 20-day period at 4 °C. SA (1.5%, w/v) or TP (0.5%, w/v) treatment alone, and the SA coating infused with TP (SA-TP) all reduced microbial counts, with the SA-TP providing the greatest effect.
29799117	1	39	theme	food	233:236	arg1	industry					238:245	the food industry	229:245	the food industry	229:245	Sodium alginate (SA) and tea polyphenols (TP) are natural preservatives commonly used in the food industry, including the production of fish products.
29799117	0	40	theme	Sea	98:100	arg1	Bass					102:105	Japanese Sea Bass	89:105	Fresh Japanese Sea Bass (Lateolabrax japonicas) Fillets	83:137	Effect of a Sodium Alginate Coating Infused with Tea Polyphenols on the Quality of Fresh Japanese Sea Bass (Lateolabrax japonicas) Fillets.
29799117	0	40	theme	Sea	98:100	arg1	japonicas					120:128	Lateolabrax japonicas	108:128	Lateolabrax japonicas	108:128	Effect of a Sodium Alginate Coating Infused with Tea Polyphenols on the Quality of Fresh Japanese Sea Bass (Lateolabrax japonicas) Fillets.
29799117	3	41	theme	protein	744:750	arg1	decomposition					752:764	protein decomposition	744:764	protein decomposition	744:764	Fish fillet samples treated with SA-TP had significantly lower levels of total volatile basic nitrogen, lipid oxidation, and protein decomposition during the storage period, relative to the remaining treatments.
29799117	0	42	theme	Coating	28:34	arg1	Effect					0:5	Effect	0:5	Effect of a Sodium Alginate Coating	0:34	Effect of a Sodium Alginate Coating Infused with Tea Polyphenols on the Quality of Fresh Japanese Sea Bass (Lateolabrax japonicas) Fillets.
29799117	0	43	theme	Lateolabrax	108:118	arg1	Bass					102:105	Japanese Sea Bass	89:105	Fresh Japanese Sea Bass (Lateolabrax japonicas) Fillets	83:137	Effect of a Sodium Alginate Coating Infused with Tea Polyphenols on the Quality of Fresh Japanese Sea Bass (Lateolabrax japonicas) Fillets.
29799117	0	43	theme	Lateolabrax	108:118	arg1	japonicas					120:128	Lateolabrax japonicas	108:128	Lateolabrax japonicas	108:128	Effect of a Sodium Alginate Coating Infused with Tea Polyphenols on the Quality of Fresh Japanese Sea Bass (Lateolabrax japonicas) Fillets.
29799117	6	44	contain	has	1156:1158	arg1	APPLICATION					1090:1100	PRACTICAL APPLICATION	1080:1100	PRACTICAL APPLICATION The sodium alginate-tea polyphenols composite coating	1080:1154	PRACTICAL APPLICATION The sodium alginate-tea polyphenols composite coating has strong potential to be used as a new biopreservative for maintaining fish fillet quality.
29799117	6	44	contain	has	1156:1158	arg2	potential					1167:1175	strong potential	1160:1175	strong potential to be used as a new biopreservative for maintaining fish fillet quality	1160:1247	PRACTICAL APPLICATION The sodium alginate-tea polyphenols composite coating has strong potential to be used as a new biopreservative for maintaining fish fillet quality.
29799117	6	45	theme	sodium	1106:1111	arg1	coating					1148:1154	The sodium alginate-tea polyphenols composite coating	1102:1154	PRACTICAL APPLICATION The sodium alginate-tea polyphenols composite coating	1080:1154	PRACTICAL APPLICATION The sodium alginate-tea polyphenols composite coating has strong potential to be used as a new biopreservative for maintaining fish fillet quality.
29799117	1	46	theme	tea	165:167	arg1	preservatives					198:210	natural preservatives	190:210	natural preservatives	190:210	Sodium alginate (SA) and tea polyphenols (TP) are natural preservatives commonly used in the food industry, including the production of fish products.
29799117	1	46	theme	tea	165:167	arg1	production					262:271	the production	258:271	the production of fish products	258:288	Sodium alginate (SA) and tea polyphenols (TP) are natural preservatives commonly used in the food industry, including the production of fish products.
29799117	1	46	theme	tea	165:167	arg1	alginate					147:154	Sodium alginate	140:154	Sodium alginate (SA)	140:159	Sodium alginate (SA) and tea polyphenols (TP) are natural preservatives commonly used in the food industry, including the production of fish products.
29799117	1	46	theme	tea	165:167	arg1	TP					182:183	TP	182:183	TP	182:183	Sodium alginate (SA) and tea polyphenols (TP) are natural preservatives commonly used in the food industry, including the production of fish products.
29799117	1	46	theme	tea	165:167	arg1	polyphenols					169:179	tea polyphenols	165:179	tea polyphenols (TP)	165:184	Sodium alginate (SA) and tea polyphenols (TP) are natural preservatives commonly used in the food industry, including the production of fish products.
29799117	0	47	theme	Alginate	19:26	arg1	Coating					28:34	a Sodium Alginate Coating	10:34	a Sodium Alginate Coating	10:34	Effect of a Sodium Alginate Coating Infused with Tea Polyphenols on the Quality of Fresh Japanese Sea Bass (Lateolabrax japonicas) Fillets.
29799117	5	48	theme	tea	961:963	arg1	polyphenols					965:975	tea polyphenols	961:975	tea polyphenols	961:975	Collectively, sodium alginate coating infused with tea polyphenols may represent a promising treatment for preservation of Japanese sea bass fillets during cold storage.
29799117	2	49	theme	4 °C.	444:448	arg1	SA					450:451	4 °C. SA (1.5%, w/v) or TP (0.5%, w/v) treatment	444:491	SA	450:451	The effect of SA coating infused with TP on the quality of fresh Japanese sea bass (Lateolabrax japonicas) fillets was evaluated over a 20-day period at 4 °C. SA (1.5%, w/v) or TP (0.5%, w/v) treatment alone, and the SA coating infused with TP (SA-TP) all reduced microbial counts, with the SA-TP providing the greatest effect.
29799117	2	50	theme	Japanese	356:363	arg1	bass					369:372	Japanese sea bass	356:372	fresh Japanese sea bass (Lateolabrax japonicas) fillets	350:404	The effect of SA coating infused with TP on the quality of fresh Japanese sea bass (Lateolabrax japonicas) fillets was evaluated over a 20-day period at 4 °C. SA (1.5%, w/v) or TP (0.5%, w/v) treatment alone, and the SA coating infused with TP (SA-TP) all reduced microbial counts, with the SA-TP providing the greatest effect.
29799117	2	50	theme	Japanese	356:363	arg1	japonicas					387:395	Lateolabrax japonicas	375:395	Lateolabrax japonicas	375:395	The effect of SA coating infused with TP on the quality of fresh Japanese sea bass (Lateolabrax japonicas) fillets was evaluated over a 20-day period at 4 °C. SA (1.5%, w/v) or TP (0.5%, w/v) treatment alone, and the SA coating infused with TP (SA-TP) all reduced microbial counts, with the SA-TP providing the greatest effect.
29799117	3	51	theme	storage	777:783	arg1	period					785:790	the storage period	773:790	the storage period	773:790	Fish fillet samples treated with SA-TP had significantly lower levels of total volatile basic nitrogen, lipid oxidation, and protein decomposition during the storage period, relative to the remaining treatments.
29799117	6	52	theme	composite	1138:1146	arg1	coating					1148:1154	The sodium alginate-tea polyphenols composite coating	1102:1154	PRACTICAL APPLICATION The sodium alginate-tea polyphenols composite coating	1080:1154	PRACTICAL APPLICATION The sodium alginate-tea polyphenols composite coating has strong potential to be used as a new biopreservative for maintaining fish fillet quality.
29799117	2	53	theme	Lateolabrax	375:385	arg1	bass					369:372	Japanese sea bass	356:372	fresh Japanese sea bass (Lateolabrax japonicas) fillets	350:404	The effect of SA coating infused with TP on the quality of fresh Japanese sea bass (Lateolabrax japonicas) fillets was evaluated over a 20-day period at 4 °C. SA (1.5%, w/v) or TP (0.5%, w/v) treatment alone, and the SA coating infused with TP (SA-TP) all reduced microbial counts, with the SA-TP providing the greatest effect.
29799117	2	53	theme	Lateolabrax	375:385	arg1	japonicas					387:395	Lateolabrax japonicas	375:395	Lateolabrax japonicas	375:395	The effect of SA coating infused with TP on the quality of fresh Japanese sea bass (Lateolabrax japonicas) fillets was evaluated over a 20-day period at 4 °C. SA (1.5%, w/v) or TP (0.5%, w/v) treatment alone, and the SA coating infused with TP (SA-TP) all reduced microbial counts, with the SA-TP providing the greatest effect.
29799117	6	54	theme	strong	1160:1165	arg1	potential					1167:1175	strong potential	1160:1175	strong potential to be used as a new biopreservative for maintaining fish fillet quality	1160:1247	PRACTICAL APPLICATION The sodium alginate-tea polyphenols composite coating has strong potential to be used as a new biopreservative for maintaining fish fillet quality.
29799117	6	55	theme	fish	1229:1232	arg1	quality					1241:1247	fish fillet quality	1229:1247	fish fillet quality	1229:1247	PRACTICAL APPLICATION The sodium alginate-tea polyphenols composite coating has strong potential to be used as a new biopreservative for maintaining fish fillet quality.
29799117	6	56	theme	PRACTICAL	1080:1088	arg1	APPLICATION					1090:1100	PRACTICAL APPLICATION	1080:1100	PRACTICAL APPLICATION The sodium alginate-tea polyphenols composite coating	1080:1154	PRACTICAL APPLICATION The sodium alginate-tea polyphenols composite coating has strong potential to be used as a new biopreservative for maintaining fish fillet quality.
29799117	4	57	contain	had	862:864	arg2	rating					894:899	the highest sensory quality rating	866:899	the highest sensory quality rating	866:899	The samples treated with SA-TP had the highest sensory quality rating as well.
29799117	4	57	contain	had	862:864	arg1	samples					835:841	The samples	831:841	The samples treated with SA-TP	831:860	The samples treated with SA-TP had the highest sensory quality rating as well.
29799117	3	58	theme	lower	676:680	arg1	levels					682:687	significantly lower levels	662:687	significantly lower levels of total volatile basic nitrogen, lipid oxidation, and protein decomposition	662:764	Fish fillet samples treated with SA-TP had significantly lower levels of total volatile basic nitrogen, lipid oxidation, and protein decomposition during the storage period, relative to the remaining treatments.
29799117	4	59	theme	quality	886:892	arg1	rating					894:899	the highest sensory quality rating	866:899	the highest sensory quality rating	866:899	The samples treated with SA-TP had the highest sensory quality rating as well.
29799117	3	60	theme	Fish	619:622	arg1	samples					631:637	Fish fillet samples	619:637	Fish fillet samples treated with SA-TP	619:656	Fish fillet samples treated with SA-TP had significantly lower levels of total volatile basic nitrogen, lipid oxidation, and protein decomposition during the storage period, relative to the remaining treatments.
29799117	0	61	theme	Fillets	131:137	arg1	Quality					72:78	the Quality	68:78	the Quality of Fresh Japanese Sea Bass (Lateolabrax japonicas) Fillets	68:137	Effect of a Sodium Alginate Coating Infused with Tea Polyphenols on the Quality of Fresh Japanese Sea Bass (Lateolabrax japonicas) Fillets.
29799117	2	62	theme	microbial	555:563	arg1	counts					565:570	microbial counts	555:570	microbial counts	555:570	The effect of SA coating infused with TP on the quality of fresh Japanese sea bass (Lateolabrax japonicas) fillets was evaluated over a 20-day period at 4 °C. SA (1.5%, w/v) or TP (0.5%, w/v) treatment alone, and the SA coating infused with TP (SA-TP) all reduced microbial counts, with the SA-TP providing the greatest effect.
29799117	3	63	theme	fillet	624:629	arg1	samples					631:637	Fish fillet samples	619:637	Fish fillet samples treated with SA-TP	619:656	Fish fillet samples treated with SA-TP had significantly lower levels of total volatile basic nitrogen, lipid oxidation, and protein decomposition during the storage period, relative to the remaining treatments.
29799117	6	64	theme	fillet	1234:1239	arg1	quality					1241:1247	fish fillet quality	1229:1247	fish fillet quality	1229:1247	PRACTICAL APPLICATION The sodium alginate-tea polyphenols composite coating has strong potential to be used as a new biopreservative for maintaining fish fillet quality.
29799117	4	65	theme	highest	870:876	arg1	rating					894:899	the highest sensory quality rating	866:899	the highest sensory quality rating	866:899	The samples treated with SA-TP had the highest sensory quality rating as well.
29799117	2	66	theme	SA	305:306	arg1	coating					308:314	SA coating	305:314	SA coating infused with TP	305:330	The effect of SA coating infused with TP on the quality of fresh Japanese sea bass (Lateolabrax japonicas) fillets was evaluated over a 20-day period at 4 °C. SA (1.5%, w/v) or TP (0.5%, w/v) treatment alone, and the SA coating infused with TP (SA-TP) all reduced microbial counts, with the SA-TP providing the greatest effect.
29799117	0	67	theme	Tea	49:51	arg1	Polyphenols					53:63	Tea Polyphenols	49:63	Tea Polyphenols	49:63	Effect of a Sodium Alginate Coating Infused with Tea Polyphenols on the Quality of Fresh Japanese Sea Bass (Lateolabrax japonicas) Fillets.
29799117	5	68	theme	alginate	931:938	arg1	coating					940:946	sodium alginate coating	924:946	sodium alginate coating infused with tea polyphenols	924:975	Collectively, sodium alginate coating infused with tea polyphenols may represent a promising treatment for preservation of Japanese sea bass fillets during cold storage.
29799117	5	69	theme	bass	1046:1049	arg1	fillets					1051:1057	Japanese sea bass fillets	1033:1057	Japanese sea bass fillets	1033:1057	Collectively, sodium alginate coating infused with tea polyphenols may represent a promising treatment for preservation of Japanese sea bass fillets during cold storage.
29799117	3	70	theme	volatile	698:705	arg1	nitrogen					713:720	total volatile basic nitrogen	692:720	total volatile basic nitrogen	692:720	Fish fillet samples treated with SA-TP had significantly lower levels of total volatile basic nitrogen, lipid oxidation, and protein decomposition during the storage period, relative to the remaining treatments.
29799117	5	71	theme	cold	1066:1069	arg1	storage					1071:1077	cold storage	1066:1077	cold storage	1066:1077	Collectively, sodium alginate coating infused with tea polyphenols may represent a promising treatment for preservation of Japanese sea bass fillets during cold storage.
31535962	3	0	theme	Phylogenetic	292:303	arg1	reconstructions					305:319	Phylogenetic reconstructions	292:319	Phylogenetic reconstructions based on 16S rRNA gene sequences and genome based on concatenated amino acid sequences of 119 single-copy genes	292:431	Phylogenetic reconstructions based on 16S rRNA gene sequences and genome based on concatenated amino acid sequences of 119 single-copy genes referred the three strains within the family Microbacteriaceae to the genus Rhodoluna.
31535962	8	1	contain	had	1303:1305	arg2	content					1317:1323	a DNA G+C content	1307:1323	a DNA G+C content of 54 mol%	1307:1334	The genomic DNA of the three strains was very small (1.4 Mbp) and had a DNA G+C content of 54 mol%.
31535962	8	1	contain	had	1303:1305	arg1	small					1283:1287	small	1283:1287	small	1283:1287	The genomic DNA of the three strains was very small (1.4 Mbp) and had a DNA G+C content of 54 mol%.
31535962	8	1	contain	had	1303:1305	arg1	Mbp					1294:1296	1.4 Mbp	1290:1296	1.4 Mbp	1290:1296	The genomic DNA of the three strains was very small (1.4 Mbp) and had a DNA G+C content of 54 mol%.
31535962	8	1	contain	had	1303:1305	arg1	DNA					1249:1251	The genomic DNA	1237:1251	The genomic DNA of the three strains	1237:1272	The genomic DNA of the three strains was very small (1.4 Mbp) and had a DNA G+C content of 54 mol%.
31535962	9	2	theme	Rhodolunalimnophila	1534:1552	arg1	sp					1554:1555	the name Rhodolunalimnophila sp	1525:1555	the name Rhodolunalimnophila sp	1525:1555	The investigated traits suggested that strains 36A-HELLB (=DSM 107805=JCM 32927), 1B-Mac (=DSM 107802=JCM 32925) and 27D-LEPIT (=JCM 32926T =DSM 107804T) represent a new species for which the name Rhodolunalimnophila sp.
31535962	7	3	theme	major	968:972	arg1	iso-C14 					991:998	iso-C14 	991:998	iso-C14 	991:998	For strain 27D-LEPIT, the major fatty acids were iso-C14 : 0 and anteiso-C15 : 0; the identified polar lipids were phosphatidylglycerol and diphosphatidylglycerol; the major respiratory quinone was MK-11; and analysis of the peptidoglycan structure indicated the presence of type B2β (typeB10).
31535962	7	3	theme	major	968:972	arg1	acids					980:984	the major fatty acids	964:984	the major fatty acids	964:984	For strain 27D-LEPIT, the major fatty acids were iso-C14 : 0 and anteiso-C15 : 0; the identified polar lipids were phosphatidylglycerol and diphosphatidylglycerol; the major respiratory quinone was MK-11; and analysis of the peptidoglycan structure indicated the presence of type B2β (typeB10).
31535962	7	4	theme	B2β	1222:1224	arg1	presence					1205:1212	the presence	1201:1212	the presence of type B2β (typeB10)	1201:1234	For strain 27D-LEPIT, the major fatty acids were iso-C14 : 0 and anteiso-C15 : 0; the identified polar lipids were phosphatidylglycerol and diphosphatidylglycerol; the major respiratory quinone was MK-11; and analysis of the peptidoglycan structure indicated the presence of type B2β (typeB10).
31535962	2	5	attach	isolated	201:208	arg2	36A-HELLB					185:193	36A-HELLB	185:193	36A-HELLB	185:193	Three actinobacterial strains, 27D-LEPIT, 1B-Mac and 36A-HELLB, were isolated from small standing and running freshwater habitats located in Salzburg, Austria.
31535962	2	5	attach	isolated	201:208	arg2	1B-Mac					174:179	1B-Mac	174:179	1B-Mac	174:179	Three actinobacterial strains, 27D-LEPIT, 1B-Mac and 36A-HELLB, were isolated from small standing and running freshwater habitats located in Salzburg, Austria.
31535962	2	5	attach	isolated	201:208	arg2	27D-LEPIT					163:171	27D-LEPIT	163:171	27D-LEPIT	163:171	Three actinobacterial strains, 27D-LEPIT, 1B-Mac and 36A-HELLB, were isolated from small standing and running freshwater habitats located in Salzburg, Austria.
31535962	2	5	attach	isolated	201:208	arg1	habitats					253:260	small standing and running freshwater habitats	215:260	small standing and running freshwater habitats located in Salzburg, Austria	215:289	Three actinobacterial strains, 27D-LEPIT, 1B-Mac and 36A-HELLB, were isolated from small standing and running freshwater habitats located in Salzburg, Austria.
31535962	2	5	attach	isolated	201:208	arg2	strains					154:160	Three actinobacterial strains	132:160	Three actinobacterial strains	132:160	Three actinobacterial strains, 27D-LEPIT, 1B-Mac and 36A-HELLB, were isolated from small standing and running freshwater habitats located in Salzburg, Austria.
31535962	7	6	theme	type	1217:1220	arg1	typeB10					1227:1233	typeB10	1227:1233	typeB10	1227:1233	For strain 27D-LEPIT, the major fatty acids were iso-C14 : 0 and anteiso-C15 : 0; the identified polar lipids were phosphatidylglycerol and diphosphatidylglycerol; the major respiratory quinone was MK-11; and analysis of the peptidoglycan structure indicated the presence of type B2β (typeB10).
31535962	7	6	theme	type	1217:1220	arg1	B2β					1222:1224	type B2β	1217:1224	type B2β (typeB10)	1217:1234	For strain 27D-LEPIT, the major fatty acids were iso-C14 : 0 and anteiso-C15 : 0; the identified polar lipids were phosphatidylglycerol and diphosphatidylglycerol; the major respiratory quinone was MK-11; and analysis of the peptidoglycan structure indicated the presence of type B2β (typeB10).
31535962	7	7	theme	major	1110:1114	arg1	MK-11					1140:1144	MK-11	1140:1144	MK-11	1140:1144	For strain 27D-LEPIT, the major fatty acids were iso-C14 : 0 and anteiso-C15 : 0; the identified polar lipids were phosphatidylglycerol and diphosphatidylglycerol; the major respiratory quinone was MK-11; and analysis of the peptidoglycan structure indicated the presence of type B2β (typeB10).
31535962	7	7	theme	major	1110:1114	arg1	quinone					1128:1134	the major respiratory quinone	1106:1134	the major respiratory quinone	1106:1134	For strain 27D-LEPIT, the major fatty acids were iso-C14 : 0 and anteiso-C15 : 0; the identified polar lipids were phosphatidylglycerol and diphosphatidylglycerol; the major respiratory quinone was MK-11; and analysis of the peptidoglycan structure indicated the presence of type B2β (typeB10).
31535962	6	8	theme	substrates	846:855	arg1	variety					835:841	a broad variety	827:841	a broad variety of substrates	827:855	The strains assimilated a broad variety of substrates; however, the patterns differed a great deal between the three investigated strains.
31535962	6	8	theme	substrates	846:855	arg1	substrates					846:855	substrates	846:855	substrates	846:855	The strains assimilated a broad variety of substrates; however, the patterns differed a great deal between the three investigated strains.
31535962	8	9	theme	DNA	1309:1311	arg1	content					1317:1323	a DNA G+C content	1307:1323	a DNA G+C content of 54 mol%	1307:1334	The genomic DNA of the three strains was very small (1.4 Mbp) and had a DNA G+C content of 54 mol%.
31535962	4	10	theme	gene	554:557	arg1	similarities					568:579	100 % 16S rRNA gene sequence similarities	539:579	100 % 16S rRNA gene sequence similarities to the previously described Candidatus Rhodoluna limnophila	539:639	The strains showed 100 % 16S rRNA gene sequence similarities to the previously described Candidatus Rhodoluna limnophila.
31535962	3	11	theme	gene	339:342	arg1	sequences					344:352	16S rRNA gene sequences	330:352	16S rRNA gene sequences	330:352	Phylogenetic reconstructions based on 16S rRNA gene sequences and genome based on concatenated amino acid sequences of 119 single-copy genes referred the three strains within the family Microbacteriaceae to the genus Rhodoluna.
31535962	6	12	theme	great	891:895	arg1	deal					897:900	a great deal	889:900	a great deal between the three investigated strains	889:939	The strains assimilated a broad variety of substrates; however, the patterns differed a great deal between the three investigated strains.
31535962	10	13	theme	type	1605:1608	arg1	strain					1610:1615	the type strain	1601:1615	the type strain	1601:1615	nov. is proposed, with strain 27D-LEPIT as the type strain.
31535962	9	14	theme	new	1503:1505	arg1	species					1507:1513	a new species	1501:1513	a new species for which the name Rhodolunalimnophila sp	1501:1555	The investigated traits suggested that strains 36A-HELLB (=DSM 107805=JCM 32927), 1B-Mac (=DSM 107802=JCM 32925) and 27D-LEPIT (=JCM 32926T =DSM 107804T) represent a new species for which the name Rhodolunalimnophila sp.
31535962	2	15	from	Austria	283:289	arg1	located					262:268	located	262:268	located	262:268	Three actinobacterial strains, 27D-LEPIT, 1B-Mac and 36A-HELLB, were isolated from small standing and running freshwater habitats located in Salzburg, Austria.
31535962	9	16	theme	investigated	1341:1352	arg1	traits					1354:1359	The investigated traits	1337:1359	The investigated traits	1337:1359	The investigated traits suggested that strains 36A-HELLB (=DSM 107805=JCM 32927), 1B-Mac (=DSM 107802=JCM 32925) and 27D-LEPIT (=JCM 32926T =DSM 107804T) represent a new species for which the name Rhodolunalimnophila sp.
31535962	5	17	theme	liquid	724:729	arg1	medium					769:774	liquid nutrient broth-soyotone-yeast extract medium	724:774	liquid nutrient broth-soyotone-yeast extract medium	724:774	Cells were very small, approximately 0.5×0.3 µm, and showed a red pigmentation in liquid nutrient broth-soyotone-yeast extract medium as well as on agar plates.
31535962	2	18	theme	located	262:268	arg1	habitats					253:260	small standing and running freshwater habitats	215:260	small standing and running freshwater habitats located in Salzburg, Austria	215:289	Three actinobacterial strains, 27D-LEPIT, 1B-Mac and 36A-HELLB, were isolated from small standing and running freshwater habitats located in Salzburg, Austria.
31535962	3	19	theme	16S	330:332	arg1	sequences					344:352	16S rRNA gene sequences	330:352	16S rRNA gene sequences	330:352	Phylogenetic reconstructions based on 16S rRNA gene sequences and genome based on concatenated amino acid sequences of 119 single-copy genes referred the three strains within the family Microbacteriaceae to the genus Rhodoluna.
31535962	1	20	from	Austria	123:129	arg1	located					102:108	located	102:108	located	102:108	nov., a bacterium with 1.4 Mbp genome size isolated from freshwater habitats located in Salzburg, Austria.
31535962	1	21	theme	Mbp	52:54	arg1	size					63:66	1.4 Mbp genome size	48:66	1.4 Mbp genome size isolated from freshwater habitats located in Salzburg, Austria	48:129	nov., a bacterium with 1.4 Mbp genome size isolated from freshwater habitats located in Salzburg, Austria.
31535962	7	22	dep	iso-C14 	991:998	arg1	indicated					1191:1199	indicated	1191:1199	indicated the presence of type B2β (typeB10)	1191:1234	For strain 27D-LEPIT, the major fatty acids were iso-C14 : 0 and anteiso-C15 : 0; the identified polar lipids were phosphatidylglycerol and diphosphatidylglycerol; the major respiratory quinone was MK-11; and analysis of the peptidoglycan structure indicated the presence of type B2β (typeB10).
31535962	7	22	dep	iso-C14 	991:998	arg1	anteiso-C15 					1007:1018	anteiso-C15 	1007:1018	anteiso-C15 	1007:1018	For strain 27D-LEPIT, the major fatty acids were iso-C14 : 0 and anteiso-C15 : 0; the identified polar lipids were phosphatidylglycerol and diphosphatidylglycerol; the major respiratory quinone was MK-11; and analysis of the peptidoglycan structure indicated the presence of type B2β (typeB10).
31535962	7	22	dep	iso-C14 	991:998	arg1	lipids					1045:1050	the identified polar lipids	1024:1050	the identified polar lipids	1024:1050	For strain 27D-LEPIT, the major fatty acids were iso-C14 : 0 and anteiso-C15 : 0; the identified polar lipids were phosphatidylglycerol and diphosphatidylglycerol; the major respiratory quinone was MK-11; and analysis of the peptidoglycan structure indicated the presence of type B2β (typeB10).
31535962	7	22	dep	iso-C14 	991:998	arg1	 0					1020:1021	 0	1020:1021	 0	1020:1021	For strain 27D-LEPIT, the major fatty acids were iso-C14 : 0 and anteiso-C15 : 0; the identified polar lipids were phosphatidylglycerol and diphosphatidylglycerol; the major respiratory quinone was MK-11; and analysis of the peptidoglycan structure indicated the presence of type B2β (typeB10).
31535962	7	22	dep	iso-C14 	991:998	arg1	phosphatidylglycerol					1057:1076	phosphatidylglycerol	1057:1076	phosphatidylglycerol	1057:1076	For strain 27D-LEPIT, the major fatty acids were iso-C14 : 0 and anteiso-C15 : 0; the identified polar lipids were phosphatidylglycerol and diphosphatidylglycerol; the major respiratory quinone was MK-11; and analysis of the peptidoglycan structure indicated the presence of type B2β (typeB10).
31535962	7	22	dep	iso-C14 	991:998	arg1	quinone					1128:1134	the major respiratory quinone	1106:1134	the major respiratory quinone	1106:1134	For strain 27D-LEPIT, the major fatty acids were iso-C14 : 0 and anteiso-C15 : 0; the identified polar lipids were phosphatidylglycerol and diphosphatidylglycerol; the major respiratory quinone was MK-11; and analysis of the peptidoglycan structure indicated the presence of type B2β (typeB10).
31535962	7	22	dep	iso-C14 	991:998	arg1	 0					1000:1001	 0	1000:1001	 0	1000:1001	For strain 27D-LEPIT, the major fatty acids were iso-C14 : 0 and anteiso-C15 : 0; the identified polar lipids were phosphatidylglycerol and diphosphatidylglycerol; the major respiratory quinone was MK-11; and analysis of the peptidoglycan structure indicated the presence of type B2β (typeB10).
31535962	7	22	dep	iso-C14 	991:998	arg1	MK-11					1140:1144	MK-11	1140:1144	MK-11	1140:1144	For strain 27D-LEPIT, the major fatty acids were iso-C14 : 0 and anteiso-C15 : 0; the identified polar lipids were phosphatidylglycerol and diphosphatidylglycerol; the major respiratory quinone was MK-11; and analysis of the peptidoglycan structure indicated the presence of type B2β (typeB10).
31535962	7	22	dep	iso-C14 	991:998	arg1	diphosphatidylglycerol					1082:1103	diphosphatidylglycerol	1082:1103	diphosphatidylglycerol	1082:1103	For strain 27D-LEPIT, the major fatty acids were iso-C14 : 0 and anteiso-C15 : 0; the identified polar lipids were phosphatidylglycerol and diphosphatidylglycerol; the major respiratory quinone was MK-11; and analysis of the peptidoglycan structure indicated the presence of type B2β (typeB10).
31535962	3	23	theme	single-copy	415:425	arg1	genes					427:431	119 single-copy genes	411:431	119 single-copy genes	411:431	Phylogenetic reconstructions based on 16S rRNA gene sequences and genome based on concatenated amino acid sequences of 119 single-copy genes referred the three strains within the family Microbacteriaceae to the genus Rhodoluna.
31535962	3	24	theme	rRNA	334:337	arg1	sequences					344:352	16S rRNA gene sequences	330:352	16S rRNA gene sequences	330:352	Phylogenetic reconstructions based on 16S rRNA gene sequences and genome based on concatenated amino acid sequences of 119 single-copy genes referred the three strains within the family Microbacteriaceae to the genus Rhodoluna.
31535962	4	25	theme	sequence	559:566	arg1	similarities					568:579	100 % 16S rRNA gene sequence similarities	539:579	100 % 16S rRNA gene sequence similarities to the previously described Candidatus Rhodoluna limnophila	539:639	The strains showed 100 % 16S rRNA gene sequence similarities to the previously described Candidatus Rhodoluna limnophila.
31535962	1	26	theme	genome	56:61	arg1	size					63:66	1.4 Mbp genome size	48:66	1.4 Mbp genome size isolated from freshwater habitats located in Salzburg, Austria	48:129	nov., a bacterium with 1.4 Mbp genome size isolated from freshwater habitats located in Salzburg, Austria.
31535962	3	27	theme	genes	427:431	arg1	sequences					398:406	concatenated amino acid sequences	374:406	concatenated amino acid sequences of 119 single-copy genes	374:431	Phylogenetic reconstructions based on 16S rRNA gene sequences and genome based on concatenated amino acid sequences of 119 single-copy genes referred the three strains within the family Microbacteriaceae to the genus Rhodoluna.
31535962	0	28	theme	limnophila	10:19	arg1	sp					21:22	Rhodoluna limnophila sp	0:22	Rhodoluna limnophila sp.	0:23	Rhodoluna limnophila sp.
31535962	9	29	theme	=JCM	1465:1468	arg1	107804T					1482:1488	=JCM 32926T =DSM 107804T	1465:1488	=JCM 32926T =DSM 107804T	1465:1488	The investigated traits suggested that strains 36A-HELLB (=DSM 107805=JCM 32927), 1B-Mac (=DSM 107802=JCM 32925) and 27D-LEPIT (=JCM 32926T =DSM 107804T) represent a new species for which the name Rhodolunalimnophila sp.
31535962	9	29	theme	=JCM	1465:1468	arg1	27D-LEPIT					1454:1462	27D-LEPIT	1454:1462	27D-LEPIT (=JCM 32926T =DSM 107804T)	1454:1489	The investigated traits suggested that strains 36A-HELLB (=DSM 107805=JCM 32927), 1B-Mac (=DSM 107802=JCM 32925) and 27D-LEPIT (=JCM 32926T =DSM 107804T) represent a new species for which the name Rhodolunalimnophila sp.
31535962	9	30	theme	32926T	1470:1475	arg1	107804T					1482:1488	=JCM 32926T =DSM 107804T	1465:1488	=JCM 32926T =DSM 107804T	1465:1488	The investigated traits suggested that strains 36A-HELLB (=DSM 107805=JCM 32927), 1B-Mac (=DSM 107802=JCM 32925) and 27D-LEPIT (=JCM 32926T =DSM 107804T) represent a new species for which the name Rhodolunalimnophila sp.
31535962	9	30	theme	32926T	1470:1475	arg1	27D-LEPIT					1454:1462	27D-LEPIT	1454:1462	27D-LEPIT (=JCM 32926T =DSM 107804T)	1454:1489	The investigated traits suggested that strains 36A-HELLB (=DSM 107805=JCM 32927), 1B-Mac (=DSM 107802=JCM 32925) and 27D-LEPIT (=JCM 32926T =DSM 107804T) represent a new species for which the name Rhodolunalimnophila sp.
31535962	0	31	theme	Rhodoluna	0:8	arg1	sp					21:22	Rhodoluna limnophila sp	0:22	Rhodoluna limnophila sp.	0:23	Rhodoluna limnophila sp.
31535962	4	32	theme	%	543:543	arg1	rRNA					549:552	100 % 16S rRNA	539:552	100 % 16S rRNA gene sequence similarities to the previously described Candidatus Rhodoluna limnophila	539:639	The strains showed 100 % 16S rRNA gene sequence similarities to the previously described Candidatus Rhodoluna limnophila.
31535962	9	33	theme	=DSM	1477:1480	arg1	107804T					1482:1488	=JCM 32926T =DSM 107804T	1465:1488	=JCM 32926T =DSM 107804T	1465:1488	The investigated traits suggested that strains 36A-HELLB (=DSM 107805=JCM 32927), 1B-Mac (=DSM 107802=JCM 32925) and 27D-LEPIT (=JCM 32926T =DSM 107804T) represent a new species for which the name Rhodolunalimnophila sp.
31535962	9	33	theme	=DSM	1477:1480	arg1	27D-LEPIT					1454:1462	27D-LEPIT	1454:1462	27D-LEPIT (=JCM 32926T =DSM 107804T)	1454:1489	The investigated traits suggested that strains 36A-HELLB (=DSM 107805=JCM 32927), 1B-Mac (=DSM 107802=JCM 32925) and 27D-LEPIT (=JCM 32926T =DSM 107804T) represent a new species for which the name Rhodolunalimnophila sp.
31535962	4	34	theme	100 	539:542	arg1	%					543:543	%	543:543	%	543:543	The strains showed 100 % 16S rRNA gene sequence similarities to the previously described Candidatus Rhodoluna limnophila.
31535962	7	35	theme	identified	1028:1037	arg1	phosphatidylglycerol					1057:1076	phosphatidylglycerol	1057:1076	phosphatidylglycerol	1057:1076	For strain 27D-LEPIT, the major fatty acids were iso-C14 : 0 and anteiso-C15 : 0; the identified polar lipids were phosphatidylglycerol and diphosphatidylglycerol; the major respiratory quinone was MK-11; and analysis of the peptidoglycan structure indicated the presence of type B2β (typeB10).
31535962	7	35	theme	identified	1028:1037	arg1	lipids					1045:1050	the identified polar lipids	1024:1050	the identified polar lipids	1024:1050	For strain 27D-LEPIT, the major fatty acids were iso-C14 : 0 and anteiso-C15 : 0; the identified polar lipids were phosphatidylglycerol and diphosphatidylglycerol; the major respiratory quinone was MK-11; and analysis of the peptidoglycan structure indicated the presence of type B2β (typeB10).
31535962	1	36	from	located	102:108	arg1	Austria					123:129	Austria	123:129	Austria	123:129	nov., a bacterium with 1.4 Mbp genome size isolated from freshwater habitats located in Salzburg, Austria.
31535962	5	37	theme	agar	790:793	arg1	plates					795:800	agar plates	790:800	agar plates	790:800	Cells were very small, approximately 0.5×0.3 µm, and showed a red pigmentation in liquid nutrient broth-soyotone-yeast extract medium as well as on agar plates.
31535962	9	38	theme	=DSM	1395:1398	arg1	36A-HELLB					1384:1392	36A-HELLB	1384:1392	36A-HELLB (=DSM 107805=JCM 32927)	1384:1416	The investigated traits suggested that strains 36A-HELLB (=DSM 107805=JCM 32927), 1B-Mac (=DSM 107802=JCM 32925) and 27D-LEPIT (=JCM 32926T =DSM 107804T) represent a new species for which the name Rhodolunalimnophila sp.
31535962	9	38	theme	=DSM	1395:1398	arg1	107805=JCM					1400:1409	=DSM 107805=JCM 32927	1395:1415	=DSM 107805=JCM 32927	1395:1415	The investigated traits suggested that strains 36A-HELLB (=DSM 107805=JCM 32927), 1B-Mac (=DSM 107802=JCM 32925) and 27D-LEPIT (=JCM 32926T =DSM 107804T) represent a new species for which the name Rhodolunalimnophila sp.
31535962	4	39	theme	rRNA	549:552	arg1	similarities					568:579	100 % 16S rRNA gene sequence similarities	539:579	100 % 16S rRNA gene sequence similarities to the previously described Candidatus Rhodoluna limnophila	539:639	The strains showed 100 % 16S rRNA gene sequence similarities to the previously described Candidatus Rhodoluna limnophila.
31535962	7	40	theme	polar	1039:1043	arg1	phosphatidylglycerol					1057:1076	phosphatidylglycerol	1057:1076	phosphatidylglycerol	1057:1076	For strain 27D-LEPIT, the major fatty acids were iso-C14 : 0 and anteiso-C15 : 0; the identified polar lipids were phosphatidylglycerol and diphosphatidylglycerol; the major respiratory quinone was MK-11; and analysis of the peptidoglycan structure indicated the presence of type B2β (typeB10).
31535962	7	40	theme	polar	1039:1043	arg1	lipids					1045:1050	the identified polar lipids	1024:1050	the identified polar lipids	1024:1050	For strain 27D-LEPIT, the major fatty acids were iso-C14 : 0 and anteiso-C15 : 0; the identified polar lipids were phosphatidylglycerol and diphosphatidylglycerol; the major respiratory quinone was MK-11; and analysis of the peptidoglycan structure indicated the presence of type B2β (typeB10).
31535962	1	41	theme	freshwater	82:91	arg1	habitats					93:100	freshwater habitats	82:100	freshwater habitats located in Salzburg, Austria	82:129	nov., a bacterium with 1.4 Mbp genome size isolated from freshwater habitats located in Salzburg, Austria.
31535962	7	42	theme	strain	946:951	arg1	27D-LEPIT					953:961	strain 27D-LEPIT	946:961	strain 27D-LEPIT	946:961	For strain 27D-LEPIT, the major fatty acids were iso-C14 : 0 and anteiso-C15 : 0; the identified polar lipids were phosphatidylglycerol and diphosphatidylglycerol; the major respiratory quinone was MK-11; and analysis of the peptidoglycan structure indicated the presence of type B2β (typeB10).
31535962	4	43	theme	Rhodoluna	620:628	arg1	limnophila					630:639	the previously described Candidatus Rhodoluna limnophila	584:639	the previously described Candidatus Rhodoluna limnophila	584:639	The strains showed 100 % 16S rRNA gene sequence similarities to the previously described Candidatus Rhodoluna limnophila.
31535962	3	44	theme	acid	393:396	arg1	sequences					398:406	concatenated amino acid sequences	374:406	concatenated amino acid sequences of 119 single-copy genes	374:431	Phylogenetic reconstructions based on 16S rRNA gene sequences and genome based on concatenated amino acid sequences of 119 single-copy genes referred the three strains within the family Microbacteriaceae to the genus Rhodoluna.
31535962	4	45	theme	16S	545:547	arg1	rRNA					549:552	100 % 16S rRNA	539:552	100 % 16S rRNA gene sequence similarities to the previously described Candidatus Rhodoluna limnophila	539:639	The strains showed 100 % 16S rRNA gene sequence similarities to the previously described Candidatus Rhodoluna limnophila.
31535962	5	46	theme	red	704:706	arg1	pigmentation					708:719	a red pigmentation	702:719	a red pigmentation	702:719	Cells were very small, approximately 0.5×0.3 µm, and showed a red pigmentation in liquid nutrient broth-soyotone-yeast extract medium as well as on agar plates.
31535962	9	47	dep	strains	1376:1382	arg1	107802=JCM					1432:1441	=DSM 107802=JCM 32925	1427:1447	=DSM 107802=JCM 32925	1427:1447	The investigated traits suggested that strains 36A-HELLB (=DSM 107805=JCM 32927), 1B-Mac (=DSM 107802=JCM 32925) and 27D-LEPIT (=JCM 32926T =DSM 107804T) represent a new species for which the name Rhodolunalimnophila sp.
31535962	9	47	dep	strains	1376:1382	arg1	strains					1376:1382	strains	1376:1382	strains 36A-HELLB (=DSM 107805=JCM 32927), 1B-Mac (=DSM 107802=JCM 32925) and 27D-LEPIT (=JCM 32926T =DSM 107804T)	1376:1489	The investigated traits suggested that strains 36A-HELLB (=DSM 107805=JCM 32927), 1B-Mac (=DSM 107802=JCM 32925) and 27D-LEPIT (=JCM 32926T =DSM 107804T) represent a new species for which the name Rhodolunalimnophila sp.
31535962	9	47	dep	strains	1376:1382	arg1	36A-HELLB					1384:1392	36A-HELLB	1384:1392	36A-HELLB (=DSM 107805=JCM 32927)	1384:1416	The investigated traits suggested that strains 36A-HELLB (=DSM 107805=JCM 32927), 1B-Mac (=DSM 107802=JCM 32925) and 27D-LEPIT (=JCM 32926T =DSM 107804T) represent a new species for which the name Rhodolunalimnophila sp.
31535962	9	47	dep	strains	1376:1382	arg1	107804T					1482:1488	=JCM 32926T =DSM 107804T	1465:1488	=JCM 32926T =DSM 107804T	1465:1488	The investigated traits suggested that strains 36A-HELLB (=DSM 107805=JCM 32927), 1B-Mac (=DSM 107802=JCM 32925) and 27D-LEPIT (=JCM 32926T =DSM 107804T) represent a new species for which the name Rhodolunalimnophila sp.
31535962	9	47	dep	strains	1376:1382	arg1	1B-Mac					1419:1424	1B-Mac	1419:1424	1B-Mac (=DSM 107802=JCM 32925)	1419:1448	The investigated traits suggested that strains 36A-HELLB (=DSM 107805=JCM 32927), 1B-Mac (=DSM 107802=JCM 32925) and 27D-LEPIT (=JCM 32926T =DSM 107804T) represent a new species for which the name Rhodolunalimnophila sp.
31535962	9	47	dep	strains	1376:1382	arg1	27D-LEPIT					1454:1462	27D-LEPIT	1454:1462	27D-LEPIT (=JCM 32926T =DSM 107804T)	1454:1489	The investigated traits suggested that strains 36A-HELLB (=DSM 107805=JCM 32927), 1B-Mac (=DSM 107802=JCM 32925) and 27D-LEPIT (=JCM 32926T =DSM 107804T) represent a new species for which the name Rhodolunalimnophila sp.
31535962	9	47	dep	strains	1376:1382	arg1	107805=JCM					1400:1409	=DSM 107805=JCM 32927	1395:1415	=DSM 107805=JCM 32927	1395:1415	The investigated traits suggested that strains 36A-HELLB (=DSM 107805=JCM 32927), 1B-Mac (=DSM 107802=JCM 32925) and 27D-LEPIT (=JCM 32926T =DSM 107804T) represent a new species for which the name Rhodolunalimnophila sp.
31535962	7	48	theme	respiratory	1116:1126	arg1	MK-11					1140:1144	MK-11	1140:1144	MK-11	1140:1144	For strain 27D-LEPIT, the major fatty acids were iso-C14 : 0 and anteiso-C15 : 0; the identified polar lipids were phosphatidylglycerol and diphosphatidylglycerol; the major respiratory quinone was MK-11; and analysis of the peptidoglycan structure indicated the presence of type B2β (typeB10).
31535962	7	48	theme	respiratory	1116:1126	arg1	quinone					1128:1134	the major respiratory quinone	1106:1134	the major respiratory quinone	1106:1134	For strain 27D-LEPIT, the major fatty acids were iso-C14 : 0 and anteiso-C15 : 0; the identified polar lipids were phosphatidylglycerol and diphosphatidylglycerol; the major respiratory quinone was MK-11; and analysis of the peptidoglycan structure indicated the presence of type B2β (typeB10).
31535962	1	49	attach	isolated	68:75	arg1	habitats					93:100	freshwater habitats	82:100	freshwater habitats located in Salzburg, Austria	82:129	nov., a bacterium with 1.4 Mbp genome size isolated from freshwater habitats located in Salzburg, Austria.
31535962	1	49	attach	isolated	68:75	arg2	size					63:66	1.4 Mbp genome size	48:66	1.4 Mbp genome size isolated from freshwater habitats located in Salzburg, Austria	48:129	nov., a bacterium with 1.4 Mbp genome size isolated from freshwater habitats located in Salzburg, Austria.
31535962	8	50	dep	small	1283:1287	arg1	small					1283:1287	small	1283:1287	small	1283:1287	The genomic DNA of the three strains was very small (1.4 Mbp) and had a DNA G+C content of 54 mol%.
31535962	8	50	dep	small	1283:1287	arg1	Mbp					1294:1296	1.4 Mbp	1290:1296	1.4 Mbp	1290:1296	The genomic DNA of the three strains was very small (1.4 Mbp) and had a DNA G+C content of 54 mol%.
31535962	8	50	dep	small	1283:1287	arg1	DNA					1249:1251	The genomic DNA	1237:1251	The genomic DNA of the three strains	1237:1272	The genomic DNA of the three strains was very small (1.4 Mbp) and had a DNA G+C content of 54 mol%.
31535962	5	51	theme	broth-soyotone-yeast	740:759	arg1	medium					769:774	liquid nutrient broth-soyotone-yeast extract medium	724:774	liquid nutrient broth-soyotone-yeast extract medium	724:774	Cells were very small, approximately 0.5×0.3 µm, and showed a red pigmentation in liquid nutrient broth-soyotone-yeast extract medium as well as on agar plates.
31535962	3	52	theme	concatenated	374:385	arg1	sequences					398:406	concatenated amino acid sequences	374:406	concatenated amino acid sequences of 119 single-copy genes	374:431	Phylogenetic reconstructions based on 16S rRNA gene sequences and genome based on concatenated amino acid sequences of 119 single-copy genes referred the three strains within the family Microbacteriaceae to the genus Rhodoluna.
31535962	7	53	theme	structure	1181:1189	arg1	analysis					1151:1158	analysis	1151:1158	analysis of the peptidoglycan structure	1151:1189	For strain 27D-LEPIT, the major fatty acids were iso-C14 : 0 and anteiso-C15 : 0; the identified polar lipids were phosphatidylglycerol and diphosphatidylglycerol; the major respiratory quinone was MK-11; and analysis of the peptidoglycan structure indicated the presence of type B2β (typeB10).
31535962	2	54	theme	actinobacterial	138:152	arg1	1B-Mac					174:179	1B-Mac	174:179	1B-Mac	174:179	Three actinobacterial strains, 27D-LEPIT, 1B-Mac and 36A-HELLB, were isolated from small standing and running freshwater habitats located in Salzburg, Austria.
31535962	2	54	theme	actinobacterial	138:152	arg1	27D-LEPIT					163:171	27D-LEPIT	163:171	27D-LEPIT	163:171	Three actinobacterial strains, 27D-LEPIT, 1B-Mac and 36A-HELLB, were isolated from small standing and running freshwater habitats located in Salzburg, Austria.
31535962	2	54	theme	actinobacterial	138:152	arg1	strains					154:160	Three actinobacterial strains	132:160	Three actinobacterial strains	132:160	Three actinobacterial strains, 27D-LEPIT, 1B-Mac and 36A-HELLB, were isolated from small standing and running freshwater habitats located in Salzburg, Austria.
31535962	2	54	theme	actinobacterial	138:152	arg1	36A-HELLB					185:193	36A-HELLB	185:193	36A-HELLB	185:193	Three actinobacterial strains, 27D-LEPIT, 1B-Mac and 36A-HELLB, were isolated from small standing and running freshwater habitats located in Salzburg, Austria.
31535962	8	55	theme	%	1334:1334	arg1	content					1317:1323	a DNA G+C content	1307:1323	a DNA G+C content of 54 mol%	1307:1334	The genomic DNA of the three strains was very small (1.4 Mbp) and had a DNA G+C content of 54 mol%.
31535962	3	56	theme	family	471:476	arg1	Microbacteriaceae					478:494	the family Microbacteriaceae	467:494	the family Microbacteriaceae	467:494	Phylogenetic reconstructions based on 16S rRNA gene sequences and genome based on concatenated amino acid sequences of 119 single-copy genes referred the three strains within the family Microbacteriaceae to the genus Rhodoluna.
31535962	3	57	theme	amino	387:391	arg1	sequences					398:406	concatenated amino acid sequences	374:406	concatenated amino acid sequences of 119 single-copy genes	374:431	Phylogenetic reconstructions based on 16S rRNA gene sequences and genome based on concatenated amino acid sequences of 119 single-copy genes referred the three strains within the family Microbacteriaceae to the genus Rhodoluna.
31535962	6	58	theme	investigated	920:931	arg1	strains					933:939	the three investigated strains	910:939	the three investigated strains	910:939	The strains assimilated a broad variety of substrates; however, the patterns differed a great deal between the three investigated strains.
31535962	7	59	theme	peptidoglycan	1167:1179	arg1	structure					1181:1189	the peptidoglycan structure	1163:1189	the peptidoglycan structure	1163:1189	For strain 27D-LEPIT, the major fatty acids were iso-C14 : 0 and anteiso-C15 : 0; the identified polar lipids were phosphatidylglycerol and diphosphatidylglycerol; the major respiratory quinone was MK-11; and analysis of the peptidoglycan structure indicated the presence of type B2β (typeB10).
31535962	8	60	theme	54 mol	1328:1333	arg1	%					1334:1334	54 mol%	1328:1334	54 mol%	1328:1334	The genomic DNA of the three strains was very small (1.4 Mbp) and had a DNA G+C content of 54 mol%.
31535962	1	61	theme	located	102:108	arg1	habitats					93:100	freshwater habitats	82:100	freshwater habitats located in Salzburg, Austria	82:129	nov., a bacterium with 1.4 Mbp genome size isolated from freshwater habitats located in Salzburg, Austria.
31535962	9	62	theme	name	1529:1532	arg1	sp					1554:1555	the name Rhodolunalimnophila sp	1525:1555	the name Rhodolunalimnophila sp	1525:1555	The investigated traits suggested that strains 36A-HELLB (=DSM 107805=JCM 32927), 1B-Mac (=DSM 107802=JCM 32925) and 27D-LEPIT (=JCM 32926T =DSM 107804T) represent a new species for which the name Rhodolunalimnophila sp.
31535962	2	63	theme	running	234:240	arg1	habitats					253:260	small standing and running freshwater habitats	215:260	small standing and running freshwater habitats located in Salzburg, Austria	215:289	Three actinobacterial strains, 27D-LEPIT, 1B-Mac and 36A-HELLB, were isolated from small standing and running freshwater habitats located in Salzburg, Austria.
31535962	8	64	theme	strains	1266:1272	arg1	small					1283:1287	small	1283:1287	small	1283:1287	The genomic DNA of the three strains was very small (1.4 Mbp) and had a DNA G+C content of 54 mol%.
31535962	8	64	theme	strains	1266:1272	arg1	Mbp					1294:1296	1.4 Mbp	1290:1296	1.4 Mbp	1290:1296	The genomic DNA of the three strains was very small (1.4 Mbp) and had a DNA G+C content of 54 mol%.
31535962	8	64	theme	strains	1266:1272	arg1	DNA					1249:1251	The genomic DNA	1237:1251	The genomic DNA of the three strains	1237:1272	The genomic DNA of the three strains was very small (1.4 Mbp) and had a DNA G+C content of 54 mol%.
31535962	8	65	theme	G+C	1313:1315	arg1	content					1317:1323	a DNA G+C content	1307:1323	a DNA G+C content of 54 mol%	1307:1334	The genomic DNA of the three strains was very small (1.4 Mbp) and had a DNA G+C content of 54 mol%.
31535962	5	66	theme	nutrient	731:738	arg1	medium					769:774	liquid nutrient broth-soyotone-yeast extract medium	724:774	liquid nutrient broth-soyotone-yeast extract medium	724:774	Cells were very small, approximately 0.5×0.3 µm, and showed a red pigmentation in liquid nutrient broth-soyotone-yeast extract medium as well as on agar plates.
31535962	2	67	theme	freshwater	242:251	arg1	habitats					253:260	small standing and running freshwater habitats	215:260	small standing and running freshwater habitats located in Salzburg, Austria	215:289	Three actinobacterial strains, 27D-LEPIT, 1B-Mac and 36A-HELLB, were isolated from small standing and running freshwater habitats located in Salzburg, Austria.
31535962	6	68	theme	broad	829:833	arg1	variety					835:841	a broad variety	827:841	a broad variety of substrates	827:855	The strains assimilated a broad variety of substrates; however, the patterns differed a great deal between the three investigated strains.
31535962	6	68	theme	broad	829:833	arg1	substrates					846:855	substrates	846:855	substrates	846:855	The strains assimilated a broad variety of substrates; however, the patterns differed a great deal between the three investigated strains.
31535962	7	69	theme	fatty	974:978	arg1	iso-C14 					991:998	iso-C14 	991:998	iso-C14 	991:998	For strain 27D-LEPIT, the major fatty acids were iso-C14 : 0 and anteiso-C15 : 0; the identified polar lipids were phosphatidylglycerol and diphosphatidylglycerol; the major respiratory quinone was MK-11; and analysis of the peptidoglycan structure indicated the presence of type B2β (typeB10).
31535962	7	69	theme	fatty	974:978	arg1	acids					980:984	the major fatty acids	964:984	the major fatty acids	964:984	For strain 27D-LEPIT, the major fatty acids were iso-C14 : 0 and anteiso-C15 : 0; the identified polar lipids were phosphatidylglycerol and diphosphatidylglycerol; the major respiratory quinone was MK-11; and analysis of the peptidoglycan structure indicated the presence of type B2β (typeB10).
31535962	10	70	theme	strain	1581:1586	arg1	27D-LEPIT					1588:1596	strain 27D-LEPIT	1581:1596	strain 27D-LEPIT as the type strain	1581:1615	nov. is proposed, with strain 27D-LEPIT as the type strain.
31535962	5	71	theme	extract	761:767	arg1	medium					769:774	liquid nutrient broth-soyotone-yeast extract medium	724:774	liquid nutrient broth-soyotone-yeast extract medium	724:774	Cells were very small, approximately 0.5×0.3 µm, and showed a red pigmentation in liquid nutrient broth-soyotone-yeast extract medium as well as on agar plates.
31535962	8	72	theme	genomic	1241:1247	arg1	small					1283:1287	small	1283:1287	small	1283:1287	The genomic DNA of the three strains was very small (1.4 Mbp) and had a DNA G+C content of 54 mol%.
31535962	8	72	theme	genomic	1241:1247	arg1	Mbp					1294:1296	1.4 Mbp	1290:1296	1.4 Mbp	1290:1296	The genomic DNA of the three strains was very small (1.4 Mbp) and had a DNA G+C content of 54 mol%.
31535962	8	72	theme	genomic	1241:1247	arg1	DNA					1249:1251	The genomic DNA	1237:1251	The genomic DNA of the three strains	1237:1272	The genomic DNA of the three strains was very small (1.4 Mbp) and had a DNA G+C content of 54 mol%.
31535962	2	73	theme	standing	221:228	arg1	habitats					253:260	small standing and running freshwater habitats	215:260	small standing and running freshwater habitats located in Salzburg, Austria	215:289	Three actinobacterial strains, 27D-LEPIT, 1B-Mac and 36A-HELLB, were isolated from small standing and running freshwater habitats located in Salzburg, Austria.
31535962	9	74	theme	=DSM	1427:1430	arg1	1B-Mac					1419:1424	1B-Mac	1419:1424	1B-Mac (=DSM 107802=JCM 32925)	1419:1448	The investigated traits suggested that strains 36A-HELLB (=DSM 107805=JCM 32927), 1B-Mac (=DSM 107802=JCM 32925) and 27D-LEPIT (=JCM 32926T =DSM 107804T) represent a new species for which the name Rhodolunalimnophila sp.
31535962	9	74	theme	=DSM	1427:1430	arg1	107802=JCM					1432:1441	=DSM 107802=JCM 32925	1427:1447	=DSM 107802=JCM 32925	1427:1447	The investigated traits suggested that strains 36A-HELLB (=DSM 107805=JCM 32927), 1B-Mac (=DSM 107802=JCM 32925) and 27D-LEPIT (=JCM 32926T =DSM 107804T) represent a new species for which the name Rhodolunalimnophila sp.
31535962	3	75	theme	genus	503:507	arg1	Rhodoluna					509:517	the genus Rhodoluna	499:517	the genus Rhodoluna	499:517	Phylogenetic reconstructions based on 16S rRNA gene sequences and genome based on concatenated amino acid sequences of 119 single-copy genes referred the three strains within the family Microbacteriaceae to the genus Rhodoluna.
31535962	2	76	from	located	262:268	arg1	Austria					283:289	Austria	283:289	Austria	283:289	Three actinobacterial strains, 27D-LEPIT, 1B-Mac and 36A-HELLB, were isolated from small standing and running freshwater habitats located in Salzburg, Austria.
31535962	4	77	theme	Candidatus	609:618	arg1	limnophila					630:639	the previously described Candidatus Rhodoluna limnophila	584:639	the previously described Candidatus Rhodoluna limnophila	584:639	The strains showed 100 % 16S rRNA gene sequence similarities to the previously described Candidatus Rhodoluna limnophila.
31535962	1	78	with	bacterium	33:41	arg1	size					63:66	1.4 Mbp genome size	48:66	1.4 Mbp genome size isolated from freshwater habitats located in Salzburg, Austria	48:129	nov., a bacterium with 1.4 Mbp genome size isolated from freshwater habitats located in Salzburg, Austria.
31535962	2	79	theme	small	215:219	arg1	habitats					253:260	small standing and running freshwater habitats	215:260	small standing and running freshwater habitats located in Salzburg, Austria	215:289	Three actinobacterial strains, 27D-LEPIT, 1B-Mac and 36A-HELLB, were isolated from small standing and running freshwater habitats located in Salzburg, Austria.
31535962	4	80	theme	described	599:607	arg1	limnophila					630:639	the previously described Candidatus Rhodoluna limnophila	584:639	the previously described Candidatus Rhodoluna limnophila	584:639	The strains showed 100 % 16S rRNA gene sequence similarities to the previously described Candidatus Rhodoluna limnophila.
31429816	3	0	theme	sequence	319:326	arg1	analysis					287:294	Comparative analysis	275:294	Comparative analysis of the almost-complete sequence of the 16S rRNA gene	275:347	Comparative analysis of the almost-complete sequence of the 16S rRNA gene showed that the closest relatives were Hungateiclostridium straminisolvens CSK1T (97.53 %) and Hungateiclostridium thermocellum DSM 2360T (95.42 %).
31429816	11	1	theme	type	1220:1223	arg1	N2K1T					1233:1237	N2K1T	1233:1237	N2K1T	1233:1237	The type strain, N2K1T, was isolated from a mesophilically operated, lab-scale biogas fermenter fed with maize silage in Freising, Germany in 2017.
31429816	11	1	theme	type	1220:1223	arg1	strain					1225:1230	The type strain	1216:1230	The type strain	1216:1230	The type strain, N2K1T, was isolated from a mesophilically operated, lab-scale biogas fermenter fed with maize silage in Freising, Germany in 2017.
31429816	9	2	theme	main	1075:1078	arg1	products					1093:1100	The main fermentation products	1071:1100	The main fermentation products	1071:1100	The main fermentation products were acetic acid and isopropanol.
31429816	9	2	theme	main	1075:1078	arg1	acid					1114:1117	acetic acid	1107:1117	acetic acid	1107:1117	The main fermentation products were acetic acid and isopropanol.
31429816	4	3	theme	new	578:580	arg1	species					582:588	a new species	576:588	a new species	576:588	Due to physiological and phylogenetic differences from its closest relatives, a new species is proposed.
31429816	13	4	theme	name	1454:1457	arg1	sp					1489:1490	The name Hungateiclostridiummesophilum sp	1450:1490	The name Hungateiclostridiummesophilum sp.	1450:1491	The name Hungateiclostridiummesophilum sp.
31429816	4	5	theme	physiological	505:517	arg1	differences					536:546	physiological and phylogenetic differences	505:546	physiological and phylogenetic differences from its closest relatives	505:573	Due to physiological and phylogenetic differences from its closest relatives, a new species is proposed.
31429816	6	6	theme	neutral	813:819	arg1	optimum					825:831	optimum	825:831	optimum	825:831	Cellulolytic activity and optimal growth were observed at 45 °C and neutral pH (optimum, pH 7.5).
31429816	6	6	theme	neutral	813:819	arg1	pH					821:822	neutral pH	813:822	neutral pH (optimum, pH 7.5)	813:840	Cellulolytic activity and optimal growth were observed at 45 °C and neutral pH (optimum, pH 7.5).
31429816	11	7	theme	maize	1321:1325	arg1	silage					1327:1332	maize silage	1321:1332	maize silage in Freising, Germany in 2017	1321:1361	The type strain, N2K1T, was isolated from a mesophilically operated, lab-scale biogas fermenter fed with maize silage in Freising, Germany in 2017.
31429816	15	8	dep	107956T	1553:1559	arg1	9704T					1568:1572	=CECT 9704T	1562:1572	=DSM 107956T; =CECT 9704T	1548:1572	Strain N2K1T (=DSM 107956T; =CECT 9704T) represents the type strain of Hungateiclostridiummesophilum sp.
31429816	15	9	theme	Strain	1534:1539	arg1	N2K1T					1541:1545	Strain N2K1T	1534:1545	Strain N2K1T (=DSM 107956T; =CECT 9704T)	1534:1573	Strain N2K1T (=DSM 107956T; =CECT 9704T) represents the type strain of Hungateiclostridiummesophilum sp.
31429816	12	10	theme	genome	1368:1373	arg1	Mbp					1408:1410	4.04 Mbp	1403:1410	4.04 Mbp with a DNA G+C content of 38.36 mol%	1403:1447	The genome assembly of strain N2K1T is 4.04 Mbp with a DNA G+C content of 38.36 mol%.
31429816	12	10	theme	genome	1368:1373	arg1	assembly					1375:1382	The genome assembly	1364:1382	The genome assembly of strain N2K1T	1364:1398	The genome assembly of strain N2K1T is 4.04 Mbp with a DNA G+C content of 38.36 mol%.
31429816	1	11	theme	cellulolytic	55:66	arg1	nov.					35:38	nov.	35:38	nov.	35:38	nov., a mesophilic, cellulolytic and spore-forming bacterium isolated from a biogas fermenter fed with maize silage.
31429816	1	11	theme	cellulolytic	55:66	arg1	bacterium					86:94	a mesophilic, cellulolytic and spore-forming bacterium	41:94	a mesophilic, cellulolytic and spore-forming bacterium isolated from a biogas fermenter	41:127	nov., a mesophilic, cellulolytic and spore-forming bacterium isolated from a biogas fermenter fed with maize silage.
31429816	11	12	theme	operated	1275:1282	arg1	fermenter					1302:1310	a mesophilically operated, lab-scale biogas fermenter	1258:1310	a mesophilically operated, lab-scale biogas fermenter fed with maize silage in Freising, Germany in 2017	1258:1361	The type strain, N2K1T, was isolated from a mesophilically operated, lab-scale biogas fermenter fed with maize silage in Freising, Germany in 2017.
31429816	3	13	theme	rRNA	339:342	arg1	gene					344:347	the 16S rRNA gene	331:347	the 16S rRNA gene	331:347	Comparative analysis of the almost-complete sequence of the 16S rRNA gene showed that the closest relatives were Hungateiclostridium straminisolvens CSK1T (97.53 %) and Hungateiclostridium thermocellum DSM 2360T (95.42 %).
31429816	3	14	dep	Hungateiclostridium	388:406	arg1	straminisolvens					408:422	straminisolvens	408:422	straminisolvens	408:422	Comparative analysis of the almost-complete sequence of the 16S rRNA gene showed that the closest relatives were Hungateiclostridium straminisolvens CSK1T (97.53 %) and Hungateiclostridium thermocellum DSM 2360T (95.42 %).
31429816	9	15	theme	acetic	1107:1112	arg1	products					1093:1100	The main fermentation products	1071:1100	The main fermentation products	1071:1100	The main fermentation products were acetic acid and isopropanol.
31429816	9	15	theme	acetic	1107:1112	arg1	acid					1114:1117	acetic acid	1107:1117	acetic acid	1107:1117	The main fermentation products were acetic acid and isopropanol.
31429816	15	16	theme	Hungateiclostridiummesophilum	1605:1633	arg1	sp					1635:1636	Hungateiclostridiummesophilum sp	1605:1636	Hungateiclostridiummesophilum sp	1605:1636	Strain N2K1T (=DSM 107956T; =CECT 9704T) represents the type strain of Hungateiclostridiummesophilum sp.
31429816	3	17	theme	gene	344:347	arg1	sequence					319:326	the almost-complete sequence	299:326	the almost-complete sequence of the 16S rRNA gene	299:347	Comparative analysis of the almost-complete sequence of the 16S rRNA gene showed that the closest relatives were Hungateiclostridium straminisolvens CSK1T (97.53 %) and Hungateiclostridium thermocellum DSM 2360T (95.42 %).
31429816	12	18	with	Mbp	1408:1410	arg1	content					1427:1433	a DNA G+C content	1417:1433	a DNA G+C content of 38.36 mol%	1417:1447	The genome assembly of strain N2K1T is 4.04 Mbp with a DNA G+C content of 38.36 mol%.
31429816	1	19	theme	spore-forming	72:84	arg1	nov.					35:38	nov.	35:38	nov.	35:38	nov., a mesophilic, cellulolytic and spore-forming bacterium isolated from a biogas fermenter fed with maize silage.
31429816	1	19	theme	spore-forming	72:84	arg1	bacterium					86:94	a mesophilic, cellulolytic and spore-forming bacterium	41:94	a mesophilic, cellulolytic and spore-forming bacterium isolated from a biogas fermenter	41:127	nov., a mesophilic, cellulolytic and spore-forming bacterium isolated from a biogas fermenter fed with maize silage.
31429816	15	20	theme	type	1590:1593	arg1	strain					1595:1600	the type strain	1586:1600	the type strain of Hungateiclostridiummesophilum sp	1586:1636	Strain N2K1T (=DSM 107956T; =CECT 9704T) represents the type strain of Hungateiclostridiummesophilum sp.
31429816	15	21	theme	sp	1635:1636	arg1	strain					1595:1600	the type strain	1586:1600	the type strain of Hungateiclostridiummesophilum sp	1586:1636	Strain N2K1T (=DSM 107956T; =CECT 9704T) represents the type strain of Hungateiclostridiummesophilum sp.
31429816	0	22	theme	mesophilum	20:29	arg1	sp					31:32	Hungateiclostridium mesophilum sp	0:32	Hungateiclostridium mesophilum sp.	0:33	Hungateiclostridium mesophilum sp.
31429816	12	23	theme	G+C	1423:1425	arg1	content					1427:1433	a DNA G+C content	1417:1433	a DNA G+C content of 38.36 mol%	1417:1447	The genome assembly of strain N2K1T is 4.04 Mbp with a DNA G+C content of 38.36 mol%.
31429816	12	24	theme	DNA	1419:1421	arg1	content					1427:1433	a DNA G+C content	1417:1433	a DNA G+C content of 38.36 mol%	1417:1447	The genome assembly of strain N2K1T is 4.04 Mbp with a DNA G+C content of 38.36 mol%.
31429816	7	25	used	used	919:922	arg2	paper					881:885	only filter paper	869:885	only filter paper (cellulose)	869:897	Of all tested substrates, only filter paper (cellulose) and cellobiose were used for growth.
31429816	7	25	used	used	919:922	arg2	cellobiose					903:912	cellobiose	903:912	cellobiose	903:912	Of all tested substrates, only filter paper (cellulose) and cellobiose were used for growth.
31429816	7	25	used	used	919:922	arg2	cellulose					888:896	cellulose	888:896	cellulose	888:896	Of all tested substrates, only filter paper (cellulose) and cellobiose were used for growth.
31429816	0	26	theme	Hungateiclostridium	0:18	arg1	sp					31:32	Hungateiclostridium mesophilum sp	0:32	Hungateiclostridium mesophilum sp.	0:33	Hungateiclostridium mesophilum sp.
31429816	4	27	theme	closest	557:563	arg1	relatives					565:573	its closest relatives	553:573	its closest relatives	553:573	Due to physiological and phylogenetic differences from its closest relatives, a new species is proposed.
31429816	15	28	dep	N2K1T	1541:1545	arg1	107956T					1553:1559	=DSM 107956T	1548:1559	=DSM 107956T; =CECT 9704T	1548:1572	Strain N2K1T (=DSM 107956T; =CECT 9704T) represents the type strain of Hungateiclostridiummesophilum sp.
31429816	5	29	theme	N2K1T	612:616	arg1	Cells					603:607	Cells	603:607	Cells of N2K1T	603:616	Cells of N2K1T were observed to be rod-shaped, non-motile, spore-forming, Gram-stain-positive and able to adhere directly to cellulose fibre.
31429816	12	30	theme	38.36 mol	1438:1446	arg1	%					1447:1447	38.36 mol%	1438:1447	38.36 mol%	1438:1447	The genome assembly of strain N2K1T is 4.04 Mbp with a DNA G+C content of 38.36 mol%.
31429816	4	31	from	relatives	565:573	arg1	differences					536:546	physiological and phylogenetic differences	505:546	physiological and phylogenetic differences from its closest relatives	505:573	Due to physiological and phylogenetic differences from its closest relatives, a new species is proposed.
31429816	6	32	theme	optimal	771:777	arg1	growth					779:784	optimal growth	771:784	optimal growth	771:784	Cellulolytic activity and optimal growth were observed at 45 °C and neutral pH (optimum, pH 7.5).
31429816	11	33	from	silage	1327:1332	arg1	2017					1358:1361	2017	1358:1361	2017	1358:1361	The type strain, N2K1T, was isolated from a mesophilically operated, lab-scale biogas fermenter fed with maize silage in Freising, Germany in 2017.
31429816	11	33	from	silage	1327:1332	arg1	Germany					1347:1353	Germany	1347:1353	Germany	1347:1353	The type strain, N2K1T, was isolated from a mesophilically operated, lab-scale biogas fermenter fed with maize silage in Freising, Germany in 2017.
31429816	9	34	theme	fermentation	1080:1091	arg1	products					1093:1100	The main fermentation products	1071:1100	The main fermentation products	1071:1100	The main fermentation products were acetic acid and isopropanol.
31429816	9	34	theme	fermentation	1080:1091	arg1	acid					1114:1117	acetic acid	1107:1117	acetic acid	1107:1117	The main fermentation products were acetic acid and isopropanol.
31429816	3	35	theme	DSM	477:479	arg1	%					494:494	95.42 %	488:494	95.42 %	488:494	Comparative analysis of the almost-complete sequence of the 16S rRNA gene showed that the closest relatives were Hungateiclostridium straminisolvens CSK1T (97.53 %) and Hungateiclostridium thermocellum DSM 2360T (95.42 %).
31429816	3	35	theme	DSM	477:479	arg1	2360T					481:485	Hungateiclostridium thermocellum DSM 2360T	444:485	Hungateiclostridium thermocellum DSM 2360T (95.42 %)	444:495	Comparative analysis of the almost-complete sequence of the 16S rRNA gene showed that the closest relatives were Hungateiclostridium straminisolvens CSK1T (97.53 %) and Hungateiclostridium thermocellum DSM 2360T (95.42 %).
31429816	3	36	theme	closest	365:371	arg1	relatives					373:381	the closest relatives	361:381	the closest relatives	361:381	Comparative analysis of the almost-complete sequence of the 16S rRNA gene showed that the closest relatives were Hungateiclostridium straminisolvens CSK1T (97.53 %) and Hungateiclostridium thermocellum DSM 2360T (95.42 %).
31429816	3	36	theme	closest	365:371	arg1	CSK1T					424:428	Hungateiclostridium straminisolvens CSK1T	388:428	Hungateiclostridium straminisolvens CSK1T (97.53 %)	388:438	Comparative analysis of the almost-complete sequence of the 16S rRNA gene showed that the closest relatives were Hungateiclostridium straminisolvens CSK1T (97.53 %) and Hungateiclostridium thermocellum DSM 2360T (95.42 %).
31429816	3	37	theme	Comparative	275:285	arg1	analysis					287:294	Comparative analysis	275:294	Comparative analysis of the almost-complete sequence of the 16S rRNA gene	275:347	Comparative analysis of the almost-complete sequence of the 16S rRNA gene showed that the closest relatives were Hungateiclostridium straminisolvens CSK1T (97.53 %) and Hungateiclostridium thermocellum DSM 2360T (95.42 %).
31429816	12	38	theme	%	1447:1447	arg1	content					1427:1433	a DNA G+C content	1417:1433	a DNA G+C content of 38.36 mol%	1417:1447	The genome assembly of strain N2K1T is 4.04 Mbp with a DNA G+C content of 38.36 mol%.
31429816	6	39	dep	optimum	825:831	arg1	pH					834:835	pH 7.5	834:839	pH 7.5	834:839	Cellulolytic activity and optimal growth were observed at 45 °C and neutral pH (optimum, pH 7.5).
31429816	13	40	theme	Hungateiclostridiummesophilum	1459:1487	arg1	sp					1489:1490	The name Hungateiclostridiummesophilum sp	1450:1490	The name Hungateiclostridiummesophilum sp.	1450:1491	The name Hungateiclostridiummesophilum sp.
31429816	14	41	theme	novel	1518:1522	arg1	organism					1524:1531	the novel organism	1514:1531	the novel organism	1514:1531	nov. is proposed for the novel organism.
31429816	11	42	theme	biogas	1295:1300	arg1	fermenter					1302:1310	a mesophilically operated, lab-scale biogas fermenter	1258:1310	a mesophilically operated, lab-scale biogas fermenter fed with maize silage in Freising, Germany in 2017	1258:1361	The type strain, N2K1T, was isolated from a mesophilically operated, lab-scale biogas fermenter fed with maize silage in Freising, Germany in 2017.
31429816	3	43	theme	Hungateiclostridium	444:462	arg1	%					494:494	95.42 %	488:494	95.42 %	488:494	Comparative analysis of the almost-complete sequence of the 16S rRNA gene showed that the closest relatives were Hungateiclostridium straminisolvens CSK1T (97.53 %) and Hungateiclostridium thermocellum DSM 2360T (95.42 %).
31429816	3	43	theme	Hungateiclostridium	444:462	arg1	2360T					481:485	Hungateiclostridium thermocellum DSM 2360T	444:485	Hungateiclostridium thermocellum DSM 2360T (95.42 %)	444:495	Comparative analysis of the almost-complete sequence of the 16S rRNA gene showed that the closest relatives were Hungateiclostridium straminisolvens CSK1T (97.53 %) and Hungateiclostridium thermocellum DSM 2360T (95.42 %).
31429816	10	44	theme	fatty	1155:1159	arg1	C16 					1179:1182	C16 	1179:1182	C16 	1179:1182	The major cellular fatty acids (>5 %) were C16 : 0iso, C16 : 0 DMA and C16 : 0.
31429816	10	44	theme	fatty	1155:1159	arg1	acids					1161:1165	The major cellular fatty acids	1136:1165	The major cellular fatty acids (>5 %)	1136:1172	The major cellular fatty acids (>5 %) were C16 : 0iso, C16 : 0 DMA and C16 : 0.
31429816	10	44	theme	fatty	1155:1159	arg1	%					1171:1171	>5 %	1168:1171	>5 %	1168:1171	The major cellular fatty acids (>5 %) were C16 : 0iso, C16 : 0 DMA and C16 : 0.
31429816	1	45	attach	isolated	96:103	arg2	bacterium					86:94	a mesophilic, cellulolytic and spore-forming bacterium	41:94	a mesophilic, cellulolytic and spore-forming bacterium isolated from a biogas fermenter	41:127	nov., a mesophilic, cellulolytic and spore-forming bacterium isolated from a biogas fermenter fed with maize silage.
31429816	1	45	attach	isolated	96:103	arg2	nov.					35:38	nov.	35:38	nov.	35:38	nov., a mesophilic, cellulolytic and spore-forming bacterium isolated from a biogas fermenter fed with maize silage.
31429816	1	45	attach	isolated	96:103	arg1	fermenter					119:127	a biogas fermenter	110:127	a biogas fermenter	110:127	nov., a mesophilic, cellulolytic and spore-forming bacterium isolated from a biogas fermenter fed with maize silage.
31429816	2	46	theme	anaerobic	212:220	arg1	bacterium					251:259	a novel anaerobic, mesophilic and cellulolytic bacterium	204:259	a novel anaerobic, mesophilic and cellulolytic bacterium	204:259	In this work, the isolation and characterization of a novel anaerobic, mesophilic and cellulolytic bacterium is described.
31429816	5	47	theme	fibre	738:742	arg1	cellulose					728:736	cellulose fibre	728:742	cellulose fibre	728:742	Cells of N2K1T were observed to be rod-shaped, non-motile, spore-forming, Gram-stain-positive and able to adhere directly to cellulose fibre.
31429816	3	48	theme	Hungateiclostridium	388:406	arg1	relatives					373:381	the closest relatives	361:381	the closest relatives	361:381	Comparative analysis of the almost-complete sequence of the 16S rRNA gene showed that the closest relatives were Hungateiclostridium straminisolvens CSK1T (97.53 %) and Hungateiclostridium thermocellum DSM 2360T (95.42 %).
31429816	3	48	theme	Hungateiclostridium	388:406	arg1	CSK1T					424:428	Hungateiclostridium straminisolvens CSK1T	388:428	Hungateiclostridium straminisolvens CSK1T (97.53 %)	388:438	Comparative analysis of the almost-complete sequence of the 16S rRNA gene showed that the closest relatives were Hungateiclostridium straminisolvens CSK1T (97.53 %) and Hungateiclostridium thermocellum DSM 2360T (95.42 %).
31429816	3	48	theme	Hungateiclostridium	388:406	arg1	%					437:437	97.53 %	431:437	97.53 %	431:437	Comparative analysis of the almost-complete sequence of the 16S rRNA gene showed that the closest relatives were Hungateiclostridium straminisolvens CSK1T (97.53 %) and Hungateiclostridium thermocellum DSM 2360T (95.42 %).
31429816	4	49	theme	phylogenetic	523:534	arg1	differences					536:546	physiological and phylogenetic differences	505:546	physiological and phylogenetic differences from its closest relatives	505:573	Due to physiological and phylogenetic differences from its closest relatives, a new species is proposed.
31429816	6	50	located	observed	791:798	arg1	optimum					825:831	optimum	825:831	optimum	825:831	Cellulolytic activity and optimal growth were observed at 45 °C and neutral pH (optimum, pH 7.5).
31429816	6	50	located	observed	791:798	arg1	45 °C					803:807	45 °C	803:807	45 °C	803:807	Cellulolytic activity and optimal growth were observed at 45 °C and neutral pH (optimum, pH 7.5).
31429816	6	50	located	observed	791:798	arg1	pH					821:822	neutral pH	813:822	neutral pH (optimum, pH 7.5)	813:840	Cellulolytic activity and optimal growth were observed at 45 °C and neutral pH (optimum, pH 7.5).
31429816	6	50	located	observed	791:798	arg2	activity					758:765	Cellulolytic activity	745:765	Cellulolytic activity	745:765	Cellulolytic activity and optimal growth were observed at 45 °C and neutral pH (optimum, pH 7.5).
31429816	6	50	located	observed	791:798	arg2	growth					779:784	optimal growth	771:784	optimal growth	771:784	Cellulolytic activity and optimal growth were observed at 45 °C and neutral pH (optimum, pH 7.5).
31429816	2	51	theme	novel	206:210	arg1	bacterium					251:259	a novel anaerobic, mesophilic and cellulolytic bacterium	204:259	a novel anaerobic, mesophilic and cellulolytic bacterium	204:259	In this work, the isolation and characterization of a novel anaerobic, mesophilic and cellulolytic bacterium is described.
31429816	11	52	dep	operated	1275:1282	arg1	lab-scale					1285:1293	lab-scale	1285:1293	lab-scale	1285:1293	The type strain, N2K1T, was isolated from a mesophilically operated, lab-scale biogas fermenter fed with maize silage in Freising, Germany in 2017.
31429816	1	53	theme	biogas	112:117	arg1	fermenter					119:127	a biogas fermenter	110:127	a biogas fermenter	110:127	nov., a mesophilic, cellulolytic and spore-forming bacterium isolated from a biogas fermenter fed with maize silage.
31429816	3	54	theme	almost-complete	303:317	arg1	sequence					319:326	the almost-complete sequence	299:326	the almost-complete sequence of the 16S rRNA gene	299:347	Comparative analysis of the almost-complete sequence of the 16S rRNA gene showed that the closest relatives were Hungateiclostridium straminisolvens CSK1T (97.53 %) and Hungateiclostridium thermocellum DSM 2360T (95.42 %).
31429816	6	55	theme	Cellulolytic	745:756	arg1	activity					758:765	Cellulolytic activity	745:765	Cellulolytic activity	745:765	Cellulolytic activity and optimal growth were observed at 45 °C and neutral pH (optimum, pH 7.5).
31429816	7	56	theme	filter	874:879	arg1	cellulose					888:896	cellulose	888:896	cellulose	888:896	Of all tested substrates, only filter paper (cellulose) and cellobiose were used for growth.
31429816	7	56	theme	filter	874:879	arg1	paper					881:885	only filter paper	869:885	only filter paper (cellulose)	869:897	Of all tested substrates, only filter paper (cellulose) and cellobiose were used for growth.
31429816	1	57	theme	mesophilic	43:52	arg1	nov.					35:38	nov.	35:38	nov.	35:38	nov., a mesophilic, cellulolytic and spore-forming bacterium isolated from a biogas fermenter fed with maize silage.
31429816	1	57	theme	mesophilic	43:52	arg1	bacterium					86:94	a mesophilic, cellulolytic and spore-forming bacterium	41:94	a mesophilic, cellulolytic and spore-forming bacterium isolated from a biogas fermenter	41:127	nov., a mesophilic, cellulolytic and spore-forming bacterium isolated from a biogas fermenter fed with maize silage.
31429816	3	58	dep	Hungateiclostridium	444:462	arg1	thermocellum					464:475	thermocellum	464:475	thermocellum	464:475	Comparative analysis of the almost-complete sequence of the 16S rRNA gene showed that the closest relatives were Hungateiclostridium straminisolvens CSK1T (97.53 %) and Hungateiclostridium thermocellum DSM 2360T (95.42 %).
31429816	2	59	theme	bacterium	251:259	arg1	characterization					184:199	characterization	184:199	characterization	184:199	In this work, the isolation and characterization of a novel anaerobic, mesophilic and cellulolytic bacterium is described.
31429816	2	59	theme	bacterium	251:259	arg1	isolation					170:178	isolation	170:178	isolation	170:178	In this work, the isolation and characterization of a novel anaerobic, mesophilic and cellulolytic bacterium is described.
31429816	11	60	attach	isolated	1244:1251	arg2	N2K1T					1233:1237	N2K1T	1233:1237	N2K1T	1233:1237	The type strain, N2K1T, was isolated from a mesophilically operated, lab-scale biogas fermenter fed with maize silage in Freising, Germany in 2017.
31429816	11	60	attach	isolated	1244:1251	arg1	fermenter					1302:1310	a mesophilically operated, lab-scale biogas fermenter	1258:1310	a mesophilically operated, lab-scale biogas fermenter fed with maize silage in Freising, Germany in 2017	1258:1361	The type strain, N2K1T, was isolated from a mesophilically operated, lab-scale biogas fermenter fed with maize silage in Freising, Germany in 2017.
31429816	11	60	attach	isolated	1244:1251	arg2	strain					1225:1230	The type strain	1216:1230	The type strain	1216:1230	The type strain, N2K1T, was isolated from a mesophilically operated, lab-scale biogas fermenter fed with maize silage in Freising, Germany in 2017.
31429816	10	61	theme	cellular	1146:1153	arg1	C16 					1179:1182	C16 	1179:1182	C16 	1179:1182	The major cellular fatty acids (>5 %) were C16 : 0iso, C16 : 0 DMA and C16 : 0.
31429816	10	61	theme	cellular	1146:1153	arg1	acids					1161:1165	The major cellular fatty acids	1136:1165	The major cellular fatty acids (>5 %)	1136:1172	The major cellular fatty acids (>5 %) were C16 : 0iso, C16 : 0 DMA and C16 : 0.
31429816	10	61	theme	cellular	1146:1153	arg1	%					1171:1171	>5 %	1168:1171	>5 %	1168:1171	The major cellular fatty acids (>5 %) were C16 : 0iso, C16 : 0 DMA and C16 : 0.
31429816	15	62	theme	=CECT	1562:1566	arg1	9704T					1568:1572	=CECT 9704T	1562:1572	=DSM 107956T; =CECT 9704T	1548:1572	Strain N2K1T (=DSM 107956T; =CECT 9704T) represents the type strain of Hungateiclostridiummesophilum sp.
31429816	2	63	theme	cellulolytic	238:249	arg1	bacterium					251:259	a novel anaerobic, mesophilic and cellulolytic bacterium	204:259	a novel anaerobic, mesophilic and cellulolytic bacterium	204:259	In this work, the isolation and characterization of a novel anaerobic, mesophilic and cellulolytic bacterium is described.
31429816	10	64	theme	major	1140:1144	arg1	C16 					1179:1182	C16 	1179:1182	C16 	1179:1182	The major cellular fatty acids (>5 %) were C16 : 0iso, C16 : 0 DMA and C16 : 0.
31429816	10	64	theme	major	1140:1144	arg1	acids					1161:1165	The major cellular fatty acids	1136:1165	The major cellular fatty acids (>5 %)	1136:1172	The major cellular fatty acids (>5 %) were C16 : 0iso, C16 : 0 DMA and C16 : 0.
31429816	10	64	theme	major	1140:1144	arg1	%					1171:1171	>5 %	1168:1171	>5 %	1168:1171	The major cellular fatty acids (>5 %) were C16 : 0iso, C16 : 0 DMA and C16 : 0.
31429816	1	65	theme	maize	138:142	arg1	silage					144:149	maize silage	138:149	maize silage	138:149	nov., a mesophilic, cellulolytic and spore-forming bacterium isolated from a biogas fermenter fed with maize silage.
31429816	7	66	theme	only	869:872	arg1	cellulose					888:896	cellulose	888:896	cellulose	888:896	Of all tested substrates, only filter paper (cellulose) and cellobiose were used for growth.
31429816	7	66	theme	only	869:872	arg1	paper					881:885	only filter paper	869:885	only filter paper (cellulose)	869:897	Of all tested substrates, only filter paper (cellulose) and cellobiose were used for growth.
31429816	12	67	theme	N2K1T	1394:1398	arg1	Mbp					1408:1410	4.04 Mbp	1403:1410	4.04 Mbp with a DNA G+C content of 38.36 mol%	1403:1447	The genome assembly of strain N2K1T is 4.04 Mbp with a DNA G+C content of 38.36 mol%.
31429816	12	67	theme	N2K1T	1394:1398	arg1	assembly					1375:1382	The genome assembly	1364:1382	The genome assembly of strain N2K1T	1364:1398	The genome assembly of strain N2K1T is 4.04 Mbp with a DNA G+C content of 38.36 mol%.
31429816	3	68	theme	16S	335:337	arg1	gene					344:347	the 16S rRNA gene	331:347	the 16S rRNA gene	331:347	Comparative analysis of the almost-complete sequence of the 16S rRNA gene showed that the closest relatives were Hungateiclostridium straminisolvens CSK1T (97.53 %) and Hungateiclostridium thermocellum DSM 2360T (95.42 %).
31429816	15	69	theme	=DSM	1548:1551	arg1	107956T					1553:1559	=DSM 107956T	1548:1559	=DSM 107956T; =CECT 9704T	1548:1572	Strain N2K1T (=DSM 107956T; =CECT 9704T) represents the type strain of Hungateiclostridiummesophilum sp.
31429816	2	70	theme	mesophilic	223:232	arg1	bacterium					251:259	a novel anaerobic, mesophilic and cellulolytic bacterium	204:259	a novel anaerobic, mesophilic and cellulolytic bacterium	204:259	In this work, the isolation and characterization of a novel anaerobic, mesophilic and cellulolytic bacterium is described.
31429816	7	71	theme	tested	850:855	arg1	substrates					857:866	all tested substrates	846:866	all tested substrates	846:866	Of all tested substrates, only filter paper (cellulose) and cellobiose were used for growth.
31429816	12	72	theme	strain	1387:1392	arg1	N2K1T					1394:1398	strain N2K1T	1387:1398	strain N2K1T	1387:1398	The genome assembly of strain N2K1T is 4.04 Mbp with a DNA G+C content of 38.36 mol%.
30799108	0	0	theme	synbiotic	66:74	arg1	combination					76:86	a synbiotic combination	64:86	a synbiotic combination in a dextran sulfate sodium-induced colitis murine model	64:143	Effect of the Lactobacillus rhamnosus strain GG and tagatose as a synbiotic combination in a dextran sulfate sodium-induced colitis murine model.
30799108	2	1	theme	-induced	448:455	arg1	model					472:476	a dextran sulfate sodium (DSS)-induced colitis murine model	418:476	a dextran sulfate sodium (DSS)-induced colitis murine model	418:476	Herein, we investigated the synbiotic interaction between the Lactobacillus rhamnosus strain GG (LGG; a probiotic strain) and tagatose (a prebiotic) in a dextran sulfate sodium (DSS)-induced colitis murine model.
30799108	1	2	theme	gastrointestinal	241:256	arg1	health					258:263	gastrointestinal health	241:263	gastrointestinal health	241:263	Synbiotics, a combination of prebiotics and probiotics, produce synergistic effects to promote gastrointestinal health.
30799108	2	3	dep	LGG	363:365	arg1	strain					380:385	a probiotic strain	368:385	LGG; a probiotic strain	363:385	Herein, we investigated the synbiotic interaction between the Lactobacillus rhamnosus strain GG (LGG; a probiotic strain) and tagatose (a prebiotic) in a dextran sulfate sodium (DSS)-induced colitis murine model.
30799108	4	4	theme	fecal	696:700	arg1	microbiota					702:711	fecal microbiota	696:711	fecal microbiota	696:711	In our pyrosequencing analysis of fecal microbiota, DSS treatment increased the abundance of Proteobacteria and decreased that of Firmicutes.
30799108	3	5	theme	food	503:506	arg1	intake					508:513	food intake	503:513	food intake	503:513	Initially, body weight, food intake, and clinical features were dramatically decreased after treatment with DSS, and the addition of LGG, tagatose, or both ameliorated these effects.
30799108	5	6	theme	microbiota	867:876	arg1	composition					878:888	the gut microbiota composition	859:888	the gut microbiota composition	859:888	When LGG and tagatose were administered as synbiotics, the gut microbiota composition recovered from the dysbiosis caused by DSS treatment.
30799108	0	7	theme	dextran	93:99	arg1	model					139:143	a dextran sulfate sodium-induced colitis murine model	91:143	a dextran sulfate sodium-induced colitis murine model	91:143	Effect of the Lactobacillus rhamnosus strain GG and tagatose as a synbiotic combination in a dextran sulfate sodium-induced colitis murine model.
30799108	6	8	theme	Lactobacillus	989:1001	arg1	abundance					963:971	the abundance	959:971	the abundance of Bacteroides, Lactobacillus, and Akkermansia	959:1018	In particular, the abundance of Bacteroides, Lactobacillus, and Akkermansia was significantly associated with probiotic, prebiotic, and synbiotic treatments.
30799108	3	9	theme	body	490:493	arg1	weight					495:500	body weight	490:500	body weight	490:500	Initially, body weight, food intake, and clinical features were dramatically decreased after treatment with DSS, and the addition of LGG, tagatose, or both ameliorated these effects.
30799108	7	10	theme	dairy	1255:1259	arg1	foods					1261:1265	dairy foods	1255:1265	dairy foods	1255:1265	Taken together, our results suggest that LGG and tagatose as synbiotics can alleviate colitis, and synbiotics could be applied as dietary supplements in dairy foods such as yogurt and cheese.
30799108	5	11	theme	DSS	929:931	arg1	treatment					933:941	DSS treatment	929:941	DSS treatment	929:941	When LGG and tagatose were administered as synbiotics, the gut microbiota composition recovered from the dysbiosis caused by DSS treatment.
30799108	4	12	theme	pyrosequencing	669:682	arg1	analysis					684:691	our pyrosequencing analysis	665:691	our pyrosequencing analysis of fecal microbiota	665:711	In our pyrosequencing analysis of fecal microbiota, DSS treatment increased the abundance of Proteobacteria and decreased that of Firmicutes.
30799108	6	13	theme	Akkermansia	1008:1018	arg1	abundance					963:971	the abundance	959:971	the abundance of Bacteroides, Lactobacillus, and Akkermansia	959:1018	In particular, the abundance of Bacteroides, Lactobacillus, and Akkermansia was significantly associated with probiotic, prebiotic, and synbiotic treatments.
30799108	3	14	theme	LGG	612:614	arg1	addition					600:607	the addition	596:607	the addition of LGG, tagatose, or both	596:633	Initially, body weight, food intake, and clinical features were dramatically decreased after treatment with DSS, and the addition of LGG, tagatose, or both ameliorated these effects.
30799108	2	15	dep	Lactobacillus	328:340	arg1	rhamnosus					342:350	rhamnosus	342:350	rhamnosus	342:350	Herein, we investigated the synbiotic interaction between the Lactobacillus rhamnosus strain GG (LGG; a probiotic strain) and tagatose (a prebiotic) in a dextran sulfate sodium (DSS)-induced colitis murine model.
30799108	2	16	theme	probiotic	370:378	arg1	strain					380:385	a probiotic strain	368:385	LGG; a probiotic strain	363:385	Herein, we investigated the synbiotic interaction between the Lactobacillus rhamnosus strain GG (LGG; a probiotic strain) and tagatose (a prebiotic) in a dextran sulfate sodium (DSS)-induced colitis murine model.
30799108	0	17	theme	Lactobacillus	14:26	arg1	GG					45:46	the Lactobacillus rhamnosus strain GG	10:46	the Lactobacillus rhamnosus strain GG	10:46	Effect of the Lactobacillus rhamnosus strain GG and tagatose as a synbiotic combination in a dextran sulfate sodium-induced colitis murine model.
30799108	0	18	theme	sodium-induced	109:122	arg1	model					139:143	a dextran sulfate sodium-induced colitis murine model	91:143	a dextran sulfate sodium-induced colitis murine model	91:143	Effect of the Lactobacillus rhamnosus strain GG and tagatose as a synbiotic combination in a dextran sulfate sodium-induced colitis murine model.
30799108	3	19	theme	clinical	520:527	arg1	features					529:536	clinical features	520:536	clinical features	520:536	Initially, body weight, food intake, and clinical features were dramatically decreased after treatment with DSS, and the addition of LGG, tagatose, or both ameliorated these effects.
30799108	0	20	theme	sulfate	101:107	arg1	model					139:143	a dextran sulfate sodium-induced colitis murine model	91:143	a dextran sulfate sodium-induced colitis murine model	91:143	Effect of the Lactobacillus rhamnosus strain GG and tagatose as a synbiotic combination in a dextran sulfate sodium-induced colitis murine model.
30799108	2	21	theme	synbiotic	294:302	arg1	interaction					304:314	the synbiotic interaction	290:314	the synbiotic interaction between the Lactobacillus rhamnosus strain GG (LGG; a probiotic strain) and tagatose (a prebiotic) in a dextran sulfate sodium (DSS)-induced colitis murine model	290:476	Herein, we investigated the synbiotic interaction between the Lactobacillus rhamnosus strain GG (LGG; a probiotic strain) and tagatose (a prebiotic) in a dextran sulfate sodium (DSS)-induced colitis murine model.
30799108	1	22	theme	probiotics	190:199	arg1	Synbiotics					146:155	Synbiotics	146:155	Synbiotics	146:155	Synbiotics, a combination of prebiotics and probiotics, produce synergistic effects to promote gastrointestinal health.
30799108	1	22	theme	probiotics	190:199	arg1	combination					160:170	a combination	158:170	a combination of prebiotics and probiotics	158:199	Synbiotics, a combination of prebiotics and probiotics, produce synergistic effects to promote gastrointestinal health.
30799108	0	23	theme	strain	38:43	arg1	GG					45:46	the Lactobacillus rhamnosus strain GG	10:46	the Lactobacillus rhamnosus strain GG	10:46	Effect of the Lactobacillus rhamnosus strain GG and tagatose as a synbiotic combination in a dextran sulfate sodium-induced colitis murine model.
30799108	0	24	theme	murine	132:137	arg1	model					139:143	a dextran sulfate sodium-induced colitis murine model	91:143	a dextran sulfate sodium-induced colitis murine model	91:143	Effect of the Lactobacillus rhamnosus strain GG and tagatose as a synbiotic combination in a dextran sulfate sodium-induced colitis murine model.
30799108	4	25	theme	Proteobacteria	755:768	arg1	abundance					742:750	the abundance	738:750	the abundance of Proteobacteria	738:768	In our pyrosequencing analysis of fecal microbiota, DSS treatment increased the abundance of Proteobacteria and decreased that of Firmicutes.
30799108	1	26	theme	prebiotics	175:184	arg1	Synbiotics					146:155	Synbiotics	146:155	Synbiotics	146:155	Synbiotics, a combination of prebiotics and probiotics, produce synergistic effects to promote gastrointestinal health.
30799108	1	26	theme	prebiotics	175:184	arg1	combination					160:170	a combination	158:170	a combination of prebiotics and probiotics	158:199	Synbiotics, a combination of prebiotics and probiotics, produce synergistic effects to promote gastrointestinal health.
30799108	7	27	theme	dietary	1232:1238	arg1	yogurt					1275:1280	yogurt	1275:1280	yogurt	1275:1280	Taken together, our results suggest that LGG and tagatose as synbiotics can alleviate colitis, and synbiotics could be applied as dietary supplements in dairy foods such as yogurt and cheese.
30799108	7	27	theme	dietary	1232:1238	arg1	synbiotics					1201:1210	synbiotics	1201:1210	synbiotics	1201:1210	Taken together, our results suggest that LGG and tagatose as synbiotics can alleviate colitis, and synbiotics could be applied as dietary supplements in dairy foods such as yogurt and cheese.
30799108	7	27	theme	dietary	1232:1238	arg1	supplements					1240:1250	dietary supplements	1232:1250	dietary supplements in dairy foods such as yogurt and cheese	1232:1291	Taken together, our results suggest that LGG and tagatose as synbiotics can alleviate colitis, and synbiotics could be applied as dietary supplements in dairy foods such as yogurt and cheese.
30799108	7	27	theme	dietary	1232:1238	arg1	cheese					1286:1291	cheese	1286:1291	cheese	1286:1291	Taken together, our results suggest that LGG and tagatose as synbiotics can alleviate colitis, and synbiotics could be applied as dietary supplements in dairy foods such as yogurt and cheese.
30799108	0	28	theme	colitis	124:130	arg1	model					139:143	a dextran sulfate sodium-induced colitis murine model	91:143	a dextran sulfate sodium-induced colitis murine model	91:143	Effect of the Lactobacillus rhamnosus strain GG and tagatose as a synbiotic combination in a dextran sulfate sodium-induced colitis murine model.
30799108	6	29	theme	Bacteroides	976:986	arg1	abundance					963:971	the abundance	959:971	the abundance of Bacteroides, Lactobacillus, and Akkermansia	959:1018	In particular, the abundance of Bacteroides, Lactobacillus, and Akkermansia was significantly associated with probiotic, prebiotic, and synbiotic treatments.
30799108	2	30	theme	colitis	457:463	arg1	model					472:476	a dextran sulfate sodium (DSS)-induced colitis murine model	418:476	a dextran sulfate sodium (DSS)-induced colitis murine model	418:476	Herein, we investigated the synbiotic interaction between the Lactobacillus rhamnosus strain GG (LGG; a probiotic strain) and tagatose (a prebiotic) in a dextran sulfate sodium (DSS)-induced colitis murine model.
30799108	7	31	from	supplements	1240:1250	arg1	foods					1261:1265	dairy foods	1255:1265	dairy foods	1255:1265	Taken together, our results suggest that LGG and tagatose as synbiotics can alleviate colitis, and synbiotics could be applied as dietary supplements in dairy foods such as yogurt and cheese.
30799108	6	32	theme	synbiotic	1080:1088	arg1	treatments					1090:1099	probiotic, prebiotic, and synbiotic treatments	1054:1099	probiotic, prebiotic, and synbiotic treatments	1054:1099	In particular, the abundance of Bacteroides, Lactobacillus, and Akkermansia was significantly associated with probiotic, prebiotic, and synbiotic treatments.
30799108	0	33	theme	GG	45:46	arg1	Effect					0:5	Effect	0:5	Effect of the Lactobacillus rhamnosus strain GG and tagatose as a synbiotic combination in a dextran sulfate sodium-induced colitis murine model.	0:144	Effect of the Lactobacillus rhamnosus strain GG and tagatose as a synbiotic combination in a dextran sulfate sodium-induced colitis murine model.
30799108	2	34	theme	strain	352:357	arg1	LGG					363:365	LGG	363:365	LGG; a probiotic strain	363:385	Herein, we investigated the synbiotic interaction between the Lactobacillus rhamnosus strain GG (LGG; a probiotic strain) and tagatose (a prebiotic) in a dextran sulfate sodium (DSS)-induced colitis murine model.
30799108	2	34	theme	strain	352:357	arg1	GG					359:360	the Lactobacillus rhamnosus strain GG	324:360	the Lactobacillus rhamnosus strain GG (LGG; a probiotic strain)	324:386	Herein, we investigated the synbiotic interaction between the Lactobacillus rhamnosus strain GG (LGG; a probiotic strain) and tagatose (a prebiotic) in a dextran sulfate sodium (DSS)-induced colitis murine model.
30799108	3	35	theme	tagatose	617:624	arg1	addition					600:607	the addition	596:607	the addition of LGG, tagatose, or both	596:633	Initially, body weight, food intake, and clinical features were dramatically decreased after treatment with DSS, and the addition of LGG, tagatose, or both ameliorated these effects.
30799108	0	36	dep	Lactobacillus	14:26	arg1	rhamnosus					28:36	Lactobacillus rhamnosus	14:36	the Lactobacillus rhamnosus strain GG	10:46	Effect of the Lactobacillus rhamnosus strain GG and tagatose as a synbiotic combination in a dextran sulfate sodium-induced colitis murine model.
30799108	5	37	theme	gut	863:865	arg1	composition					878:888	the gut microbiota composition	859:888	the gut microbiota composition	859:888	When LGG and tagatose were administered as synbiotics, the gut microbiota composition recovered from the dysbiosis caused by DSS treatment.
30799108	6	38	theme	prebiotic	1065:1073	arg1	treatments					1090:1099	probiotic, prebiotic, and synbiotic treatments	1054:1099	probiotic, prebiotic, and synbiotic treatments	1054:1099	In particular, the abundance of Bacteroides, Lactobacillus, and Akkermansia was significantly associated with probiotic, prebiotic, and synbiotic treatments.
30799108	1	39	theme	synergistic	210:220	arg1	effects					222:228	synergistic effects	210:228	synergistic effects	210:228	Synbiotics, a combination of prebiotics and probiotics, produce synergistic effects to promote gastrointestinal health.
30799108	4	40	theme	DSS	714:716	arg1	treatment					718:726	DSS treatment	714:726	DSS treatment	714:726	In our pyrosequencing analysis of fecal microbiota, DSS treatment increased the abundance of Proteobacteria and decreased that of Firmicutes.
30799108	4	41	theme	microbiota	702:711	arg1	analysis					684:691	our pyrosequencing analysis	665:691	our pyrosequencing analysis of fecal microbiota	665:711	In our pyrosequencing analysis of fecal microbiota, DSS treatment increased the abundance of Proteobacteria and decreased that of Firmicutes.
30799108	2	42	theme	Lactobacillus	328:340	arg1	LGG					363:365	LGG	363:365	LGG; a probiotic strain	363:385	Herein, we investigated the synbiotic interaction between the Lactobacillus rhamnosus strain GG (LGG; a probiotic strain) and tagatose (a prebiotic) in a dextran sulfate sodium (DSS)-induced colitis murine model.
30799108	2	42	theme	Lactobacillus	328:340	arg1	GG					359:360	the Lactobacillus rhamnosus strain GG	324:360	the Lactobacillus rhamnosus strain GG (LGG; a probiotic strain)	324:386	Herein, we investigated the synbiotic interaction between the Lactobacillus rhamnosus strain GG (LGG; a probiotic strain) and tagatose (a prebiotic) in a dextran sulfate sodium (DSS)-induced colitis murine model.
30799108	2	43	theme	murine	465:470	arg1	model					472:476	a dextran sulfate sodium (DSS)-induced colitis murine model	418:476	a dextran sulfate sodium (DSS)-induced colitis murine model	418:476	Herein, we investigated the synbiotic interaction between the Lactobacillus rhamnosus strain GG (LGG; a probiotic strain) and tagatose (a prebiotic) in a dextran sulfate sodium (DSS)-induced colitis murine model.
30799108	0	44	from	combination	76:86	arg1	model					139:143	a dextran sulfate sodium-induced colitis murine model	91:143	a dextran sulfate sodium-induced colitis murine model	91:143	Effect of the Lactobacillus rhamnosus strain GG and tagatose as a synbiotic combination in a dextran sulfate sodium-induced colitis murine model.
30799108	3	45	with	treatment	572:580	arg1	DSS					587:589	DSS	587:589	DSS	587:589	Initially, body weight, food intake, and clinical features were dramatically decreased after treatment with DSS, and the addition of LGG, tagatose, or both ameliorated these effects.
30799108	0	46	theme	tagatose	52:59	arg1	Effect					0:5	Effect	0:5	Effect of the Lactobacillus rhamnosus strain GG and tagatose as a synbiotic combination in a dextran sulfate sodium-induced colitis murine model.	0:144	Effect of the Lactobacillus rhamnosus strain GG and tagatose as a synbiotic combination in a dextran sulfate sodium-induced colitis murine model.
30799108	3	47	theme	both	630:633	arg1	addition					600:607	the addition	596:607	the addition of LGG, tagatose, or both	596:633	Initially, body weight, food intake, and clinical features were dramatically decreased after treatment with DSS, and the addition of LGG, tagatose, or both ameliorated these effects.
30799108	6	48	theme	probiotic	1054:1062	arg1	treatments					1090:1099	probiotic, prebiotic, and synbiotic treatments	1054:1099	probiotic, prebiotic, and synbiotic treatments	1054:1099	In particular, the abundance of Bacteroides, Lactobacillus, and Akkermansia was significantly associated with probiotic, prebiotic, and synbiotic treatments.
30799108	2	49	from	interaction	304:314	arg1	model					472:476	a dextran sulfate sodium (DSS)-induced colitis murine model	418:476	a dextran sulfate sodium (DSS)-induced colitis murine model	418:476	Herein, we investigated the synbiotic interaction between the Lactobacillus rhamnosus strain GG (LGG; a probiotic strain) and tagatose (a prebiotic) in a dextran sulfate sodium (DSS)-induced colitis murine model.
29616891	6	0	theme	32.4±3.4 	803:811	arg1	%					812:812	%	812:812	%	812:812	The genomic G+C content was 38.1 mol%, and DNA-DNA hybridization with DSM 4848T revealed 32.4±3.4 % similarity.
29616891	8	1	theme	peptidoglycan	947:959	arg1	A1γ					966:968	peptidoglycan type A1γ	947:968	peptidoglycan type A1γ	947:968	The cell wall contained meso-diaminopimelic acid and was of peptidoglycan type A1γ.
29616891	5	2	dep	relatives	637:645	arg1	similarity					687:696	97.4 and 97.1 % 16S rRNA gene sequence similarity	648:696	97.4 and 97.1 % 16S rRNA gene sequence similarity	648:696	16S rRNA gene and multilocus (pheS, rpoA, and atpA) sequence analysis revealed Carnobacterium mobile DSM 4848T and Carnobacterium iners LMG 26642T as the closest relatives (97.4 and 97.1 % 16S rRNA gene sequence similarity, respectively).
29616891	5	3	theme	16S	664:666	arg1	similarity					687:696	97.4 and 97.1 % 16S rRNA gene sequence similarity	648:696	97.4 and 97.1 % 16S rRNA gene sequence similarity	648:696	16S rRNA gene and multilocus (pheS, rpoA, and atpA) sequence analysis revealed Carnobacterium mobile DSM 4848T and Carnobacterium iners LMG 26642T as the closest relatives (97.4 and 97.1 % 16S rRNA gene sequence similarity, respectively).
29616891	6	4	theme	%	812:812	arg1	similarity					814:823	32.4±3.4 % similarity	803:823	32.4±3.4 % similarity	803:823	The genomic G+C content was 38.1 mol%, and DNA-DNA hybridization with DSM 4848T revealed 32.4±3.4 % similarity.
29616891	5	5	theme	Carnobacterium	554:567	arg1	26642T					615:620	Carnobacterium iners LMG 26642T	590:620	Carnobacterium iners LMG 26642T	590:620	16S rRNA gene and multilocus (pheS, rpoA, and atpA) sequence analysis revealed Carnobacterium mobile DSM 4848T and Carnobacterium iners LMG 26642T as the closest relatives (97.4 and 97.1 % 16S rRNA gene sequence similarity, respectively).
29616891	5	5	theme	Carnobacterium	554:567	arg1	4848T					580:584	Carnobacterium mobile DSM 4848T	554:584	Carnobacterium mobile DSM 4848T	554:584	16S rRNA gene and multilocus (pheS, rpoA, and atpA) sequence analysis revealed Carnobacterium mobile DSM 4848T and Carnobacterium iners LMG 26642T as the closest relatives (97.4 and 97.1 % 16S rRNA gene sequence similarity, respectively).
29616891	5	5	theme	Carnobacterium	554:567	arg1	relatives					637:645	the closest relatives	625:645	the closest relatives (97.4 and 97.1 % 16S rRNA gene sequence similarity, respectively)	625:711	16S rRNA gene and multilocus (pheS, rpoA, and atpA) sequence analysis revealed Carnobacterium mobile DSM 4848T and Carnobacterium iners LMG 26642T as the closest relatives (97.4 and 97.1 % 16S rRNA gene sequence similarity, respectively).
29616891	9	6	theme	name	1119:1122	arg1	Carnobacterium					1124:1137	the name Carnobacterium	1115:1137	the name Carnobacterium antarcticum sp	1115:1152	Based on physiological, genotypic and biochemical characteristics, strain CP1T represents a novel species of the genus Carnobacterium for which the name Carnobacterium antarcticum sp.
29616891	8	7	theme	cell	891:894	arg1	wall					896:899	The cell wall	887:899	The cell wall	887:899	The cell wall contained meso-diaminopimelic acid and was of peptidoglycan type A1γ.
29616891	5	8	theme	closest	629:635	arg1	26642T					615:620	Carnobacterium iners LMG 26642T	590:620	Carnobacterium iners LMG 26642T	590:620	16S rRNA gene and multilocus (pheS, rpoA, and atpA) sequence analysis revealed Carnobacterium mobile DSM 4848T and Carnobacterium iners LMG 26642T as the closest relatives (97.4 and 97.1 % 16S rRNA gene sequence similarity, respectively).
29616891	5	8	theme	closest	629:635	arg1	4848T					580:584	Carnobacterium mobile DSM 4848T	554:584	Carnobacterium mobile DSM 4848T	554:584	16S rRNA gene and multilocus (pheS, rpoA, and atpA) sequence analysis revealed Carnobacterium mobile DSM 4848T and Carnobacterium iners LMG 26642T as the closest relatives (97.4 and 97.1 % 16S rRNA gene sequence similarity, respectively).
29616891	5	8	theme	closest	629:635	arg1	relatives					637:645	the closest relatives	625:645	the closest relatives (97.4 and 97.1 % 16S rRNA gene sequence similarity, respectively)	625:711	16S rRNA gene and multilocus (pheS, rpoA, and atpA) sequence analysis revealed Carnobacterium mobile DSM 4848T and Carnobacterium iners LMG 26642T as the closest relatives (97.4 and 97.1 % 16S rRNA gene sequence similarity, respectively).
29616891	7	9	theme	major	830:834	arg1	C14 					863:866	C14 	863:866	C14 	863:866	The major fatty acid components were C14 : 0 and C16 : 1ω9c.
29616891	7	9	theme	major	830:834	arg1	components					847:856	The major fatty acid components	826:856	The major fatty acid components	826:856	The major fatty acid components were C14 : 0 and C16 : 1ω9c.
29616891	5	10	theme	mobile	569:574	arg1	26642T					615:620	Carnobacterium iners LMG 26642T	590:620	Carnobacterium iners LMG 26642T	590:620	16S rRNA gene and multilocus (pheS, rpoA, and atpA) sequence analysis revealed Carnobacterium mobile DSM 4848T and Carnobacterium iners LMG 26642T as the closest relatives (97.4 and 97.1 % 16S rRNA gene sequence similarity, respectively).
29616891	5	10	theme	mobile	569:574	arg1	4848T					580:584	Carnobacterium mobile DSM 4848T	554:584	Carnobacterium mobile DSM 4848T	554:584	16S rRNA gene and multilocus (pheS, rpoA, and atpA) sequence analysis revealed Carnobacterium mobile DSM 4848T and Carnobacterium iners LMG 26642T as the closest relatives (97.4 and 97.1 % 16S rRNA gene sequence similarity, respectively).
29616891	5	10	theme	mobile	569:574	arg1	relatives					637:645	the closest relatives	625:645	the closest relatives (97.4 and 97.1 % 16S rRNA gene sequence similarity, respectively)	625:711	16S rRNA gene and multilocus (pheS, rpoA, and atpA) sequence analysis revealed Carnobacterium mobile DSM 4848T and Carnobacterium iners LMG 26642T as the closest relatives (97.4 and 97.1 % 16S rRNA gene sequence similarity, respectively).
29616891	9	11	theme	novel	1063:1067	arg1	species					1069:1075	a novel species	1061:1075	a novel species of the genus Carnobacterium for which the name Carnobacterium antarcticum sp	1061:1152	Based on physiological, genotypic and biochemical characteristics, strain CP1T represents a novel species of the genus Carnobacterium for which the name Carnobacterium antarcticum sp.
29616891	5	12	dep	%	662:662	arg1	97.1 					657:661	97.1 	657:661	97.1 	657:661	16S rRNA gene and multilocus (pheS, rpoA, and atpA) sequence analysis revealed Carnobacterium mobile DSM 4848T and Carnobacterium iners LMG 26642T as the closest relatives (97.4 and 97.1 % 16S rRNA gene sequence similarity, respectively).
29616891	5	12	dep	%	662:662	arg1	97.4					648:651	97.4	648:651	97.4	648:651	16S rRNA gene and multilocus (pheS, rpoA, and atpA) sequence analysis revealed Carnobacterium mobile DSM 4848T and Carnobacterium iners LMG 26642T as the closest relatives (97.4 and 97.1 % 16S rRNA gene sequence similarity, respectively).
29616891	2	13	attach	isolated	202:209	arg1	soil					222:225	sandy soil	216:225	sandy soil near the Davis Station in Antarctica	216:262	A novel, alkaliphilic, psychrotolerant, facultative anaerobe, designated CP1T, was isolated from sandy soil near the Davis Station in Antarctica.
29616891	2	13	attach	isolated	202:209	arg2	anaerobe					171:178	A novel, alkaliphilic, psychrotolerant, facultative anaerobe	119:178	A novel, alkaliphilic, psychrotolerant, facultative anaerobe	119:178	A novel, alkaliphilic, psychrotolerant, facultative anaerobe, designated CP1T, was isolated from sandy soil near the Davis Station in Antarctica.
29616891	6	14	theme	DSM	784:786	arg1	4848T					788:792	DSM 4848T	784:792	DSM 4848T	784:792	The genomic G+C content was 38.1 mol%, and DNA-DNA hybridization with DSM 4848T revealed 32.4±3.4 % similarity.
29616891	5	15	theme	DSM	576:578	arg1	26642T					615:620	Carnobacterium iners LMG 26642T	590:620	Carnobacterium iners LMG 26642T	590:620	16S rRNA gene and multilocus (pheS, rpoA, and atpA) sequence analysis revealed Carnobacterium mobile DSM 4848T and Carnobacterium iners LMG 26642T as the closest relatives (97.4 and 97.1 % 16S rRNA gene sequence similarity, respectively).
29616891	5	15	theme	DSM	576:578	arg1	4848T					580:584	Carnobacterium mobile DSM 4848T	554:584	Carnobacterium mobile DSM 4848T	554:584	16S rRNA gene and multilocus (pheS, rpoA, and atpA) sequence analysis revealed Carnobacterium mobile DSM 4848T and Carnobacterium iners LMG 26642T as the closest relatives (97.4 and 97.1 % 16S rRNA gene sequence similarity, respectively).
29616891	5	15	theme	DSM	576:578	arg1	relatives					637:645	the closest relatives	625:645	the closest relatives (97.4 and 97.1 % 16S rRNA gene sequence similarity, respectively)	625:711	16S rRNA gene and multilocus (pheS, rpoA, and atpA) sequence analysis revealed Carnobacterium mobile DSM 4848T and Carnobacterium iners LMG 26642T as the closest relatives (97.4 and 97.1 % 16S rRNA gene sequence similarity, respectively).
29616891	7	16	theme	fatty	836:840	arg1	C14 					863:866	C14 	863:866	C14 	863:866	The major fatty acid components were C14 : 0 and C16 : 1ω9c.
29616891	7	16	theme	fatty	836:840	arg1	components					847:856	The major fatty acid components	826:856	The major fatty acid components	826:856	The major fatty acid components were C14 : 0 and C16 : 1ω9c.
29616891	7	17	theme	acid	842:845	arg1	C14 					863:866	C14 	863:866	C14 	863:866	The major fatty acid components were C14 : 0 and C16 : 1ω9c.
29616891	7	17	theme	acid	842:845	arg1	components					847:856	The major fatty acid components	826:856	The major fatty acid components	826:856	The major fatty acid components were C14 : 0 and C16 : 1ω9c.
29616891	5	18	dep	similarity	687:696	arg1	%					662:662	%	662:662	%	662:662	16S rRNA gene and multilocus (pheS, rpoA, and atpA) sequence analysis revealed Carnobacterium mobile DSM 4848T and Carnobacterium iners LMG 26642T as the closest relatives (97.4 and 97.1 % 16S rRNA gene sequence similarity, respectively).
29616891	11	19	theme	103363T=CGMCC	1203:1215	arg1	CP1T					1192:1195	CP1T	1192:1195	CP1T (=DSM 103363T=CGMCC 1.15643T)	1192:1225	The type strain is CP1T (=DSM 103363T=CGMCC 1.15643T).
29616891	11	19	theme	103363T=CGMCC	1203:1215	arg1	1.15643T					1217:1224	=DSM 103363T=CGMCC 1.15643T	1198:1224	=DSM 103363T=CGMCC 1.15643T	1198:1224	The type strain is CP1T (=DSM 103363T=CGMCC 1.15643T).
29616891	2	20	dep	novel	121:125	arg1	alkaliphilic					128:139	alkaliphilic	128:139	alkaliphilic	128:139	A novel, alkaliphilic, psychrotolerant, facultative anaerobe, designated CP1T, was isolated from sandy soil near the Davis Station in Antarctica.
29616891	2	20	dep	novel	121:125	arg1	facultative					159:169	facultative	159:169	facultative	159:169	A novel, alkaliphilic, psychrotolerant, facultative anaerobe, designated CP1T, was isolated from sandy soil near the Davis Station in Antarctica.
29616891	2	20	dep	novel	121:125	arg1	psychrotolerant					142:156	psychrotolerant	142:156	psychrotolerant	142:156	A novel, alkaliphilic, psychrotolerant, facultative anaerobe, designated CP1T, was isolated from sandy soil near the Davis Station in Antarctica.
29616891	0	21	theme	antarcticum	15:25	arg1	sp					27:28	Carnobacterium antarcticum sp	0:28	Carnobacterium antarcticum sp.	0:29	Carnobacterium antarcticum sp.
29616891	6	22	theme	DNA-DNA	757:763	arg1	hybridization					765:777	DNA-DNA hybridization	757:777	DNA-DNA hybridization with DSM 4848T	757:792	The genomic G+C content was 38.1 mol%, and DNA-DNA hybridization with DSM 4848T revealed 32.4±3.4 % similarity.
29616891	5	23	theme	sequence	527:534	arg1	analysis					536:543	16S rRNA gene and multilocus (pheS, rpoA, and atpA) sequence analysis	475:543	analysis	536:543	16S rRNA gene and multilocus (pheS, rpoA, and atpA) sequence analysis revealed Carnobacterium mobile DSM 4848T and Carnobacterium iners LMG 26642T as the closest relatives (97.4 and 97.1 % 16S rRNA gene sequence similarity, respectively).
29616891	5	24	theme	multilocus	493:502	arg1	analysis					536:543	16S rRNA gene and multilocus (pheS, rpoA, and atpA) sequence analysis	475:543	analysis	536:543	16S rRNA gene and multilocus (pheS, rpoA, and atpA) sequence analysis revealed Carnobacterium mobile DSM 4848T and Carnobacterium iners LMG 26642T as the closest relatives (97.4 and 97.1 % 16S rRNA gene sequence similarity, respectively).
29616891	5	25	theme	16S	475:477	arg1	gene					484:487	16S rRNA gene and multilocus (pheS, rpoA, and atpA) sequence analysis	475:543	gene	484:487	16S rRNA gene and multilocus (pheS, rpoA, and atpA) sequence analysis revealed Carnobacterium mobile DSM 4848T and Carnobacterium iners LMG 26642T as the closest relatives (97.4 and 97.1 % 16S rRNA gene sequence similarity, respectively).
29616891	3	26	theme	Gram-positive	302:314	arg1	staining					316:323	Gram-positive staining	302:323	Gram-positive staining	302:323	The short-rod-shaped cells displayed Gram-positive staining and did not form spores.
29616891	0	27	theme	Carnobacterium	0:13	arg1	sp					27:28	Carnobacterium antarcticum sp	0:28	Carnobacterium antarcticum sp.	0:29	Carnobacterium antarcticum sp.
29616891	11	28	theme	type	1177:1180	arg1	CP1T					1192:1195	CP1T	1192:1195	CP1T (=DSM 103363T=CGMCC 1.15643T)	1192:1225	The type strain is CP1T (=DSM 103363T=CGMCC 1.15643T).
29616891	11	28	theme	type	1177:1180	arg1	strain					1182:1187	The type strain	1173:1187	The type strain	1173:1187	The type strain is CP1T (=DSM 103363T=CGMCC 1.15643T).
29616891	9	29	theme	genus	1084:1088	arg1	Carnobacterium					1090:1103	the genus Carnobacterium	1080:1103	the genus Carnobacterium	1080:1103	Based on physiological, genotypic and biochemical characteristics, strain CP1T represents a novel species of the genus Carnobacterium for which the name Carnobacterium antarcticum sp.
29616891	1	30	theme	sandy	93:97	arg1	soil					99:102	sandy soil	93:102	sandy soil in Antarctica	93:116	nov., a psychrotolerant, alkaliphilic bacterium isolated from sandy soil in Antarctica.
29616891	5	31	theme	LMG	611:613	arg1	26642T					615:620	Carnobacterium iners LMG 26642T	590:620	Carnobacterium iners LMG 26642T	590:620	16S rRNA gene and multilocus (pheS, rpoA, and atpA) sequence analysis revealed Carnobacterium mobile DSM 4848T and Carnobacterium iners LMG 26642T as the closest relatives (97.4 and 97.1 % 16S rRNA gene sequence similarity, respectively).
29616891	5	31	theme	LMG	611:613	arg1	4848T					580:584	Carnobacterium mobile DSM 4848T	554:584	Carnobacterium mobile DSM 4848T	554:584	16S rRNA gene and multilocus (pheS, rpoA, and atpA) sequence analysis revealed Carnobacterium mobile DSM 4848T and Carnobacterium iners LMG 26642T as the closest relatives (97.4 and 97.1 % 16S rRNA gene sequence similarity, respectively).
29616891	5	31	theme	LMG	611:613	arg1	relatives					637:645	the closest relatives	625:645	the closest relatives (97.4 and 97.1 % 16S rRNA gene sequence similarity, respectively)	625:711	16S rRNA gene and multilocus (pheS, rpoA, and atpA) sequence analysis revealed Carnobacterium mobile DSM 4848T and Carnobacterium iners LMG 26642T as the closest relatives (97.4 and 97.1 % 16S rRNA gene sequence similarity, respectively).
29616891	9	32	theme	Carnobacterium	1090:1103	arg1	species					1069:1075	a novel species	1061:1075	a novel species of the genus Carnobacterium for which the name Carnobacterium antarcticum sp	1061:1152	Based on physiological, genotypic and biochemical characteristics, strain CP1T represents a novel species of the genus Carnobacterium for which the name Carnobacterium antarcticum sp.
29616891	5	33	theme	rRNA	479:482	arg1	gene					484:487	16S rRNA gene and multilocus (pheS, rpoA, and atpA) sequence analysis	475:543	gene	484:487	16S rRNA gene and multilocus (pheS, rpoA, and atpA) sequence analysis revealed Carnobacterium mobile DSM 4848T and Carnobacterium iners LMG 26642T as the closest relatives (97.4 and 97.1 % 16S rRNA gene sequence similarity, respectively).
29616891	2	34	from	Station	242:248	arg1	Antarctica					253:262	Antarctica	253:262	Antarctica	253:262	A novel, alkaliphilic, psychrotolerant, facultative anaerobe, designated CP1T, was isolated from sandy soil near the Davis Station in Antarctica.
29616891	1	35	dep	psychrotolerant	39:53	arg1	alkaliphilic					56:67	alkaliphilic	56:67	alkaliphilic	56:67	nov., a psychrotolerant, alkaliphilic bacterium isolated from sandy soil in Antarctica.
29616891	5	36	theme	rRNA	668:671	arg1	similarity					687:696	97.4 and 97.1 % 16S rRNA gene sequence similarity	648:696	97.4 and 97.1 % 16S rRNA gene sequence similarity	648:696	16S rRNA gene and multilocus (pheS, rpoA, and atpA) sequence analysis revealed Carnobacterium mobile DSM 4848T and Carnobacterium iners LMG 26642T as the closest relatives (97.4 and 97.1 % 16S rRNA gene sequence similarity, respectively).
29616891	9	37	theme	physiological	980:992	arg1	characteristics					1021:1035	physiological, genotypic and biochemical characteristics	980:1035	physiological, genotypic and biochemical characteristics	980:1035	Based on physiological, genotypic and biochemical characteristics, strain CP1T represents a novel species of the genus Carnobacterium for which the name Carnobacterium antarcticum sp.
29616891	5	38	theme	gene	673:676	arg1	similarity					687:696	97.4 and 97.1 % 16S rRNA gene sequence similarity	648:696	97.4 and 97.1 % 16S rRNA gene sequence similarity	648:696	16S rRNA gene and multilocus (pheS, rpoA, and atpA) sequence analysis revealed Carnobacterium mobile DSM 4848T and Carnobacterium iners LMG 26642T as the closest relatives (97.4 and 97.1 % 16S rRNA gene sequence similarity, respectively).
29616891	4	39	dep	%	461:461	arg1	5.0 					457:460	5.0 	457:460	5.0 	457:460	Strain CP1T was able to grow at temperatures between 4 and 36 °C, pH 6.0-9.5, and in the presence of up to 5.0 % (w/v) NaCl.
29616891	4	39	dep	%	461:461	arg1	w/v					464:466	w/v	464:466	w/v	464:466	Strain CP1T was able to grow at temperatures between 4 and 36 °C, pH 6.0-9.5, and in the presence of up to 5.0 % (w/v) NaCl.
29616891	5	40	dep	analysis	536:543	arg1	rpoA					511:514	rpoA	511:514	rpoA	511:514	16S rRNA gene and multilocus (pheS, rpoA, and atpA) sequence analysis revealed Carnobacterium mobile DSM 4848T and Carnobacterium iners LMG 26642T as the closest relatives (97.4 and 97.1 % 16S rRNA gene sequence similarity, respectively).
29616891	5	40	dep	analysis	536:543	arg1	atpA					521:524	atpA	521:524	atpA	521:524	16S rRNA gene and multilocus (pheS, rpoA, and atpA) sequence analysis revealed Carnobacterium mobile DSM 4848T and Carnobacterium iners LMG 26642T as the closest relatives (97.4 and 97.1 % 16S rRNA gene sequence similarity, respectively).
29616891	5	40	dep	analysis	536:543	arg1	pheS					505:508	pheS	505:508	pheS	505:508	16S rRNA gene and multilocus (pheS, rpoA, and atpA) sequence analysis revealed Carnobacterium mobile DSM 4848T and Carnobacterium iners LMG 26642T as the closest relatives (97.4 and 97.1 % 16S rRNA gene sequence similarity, respectively).
29616891	2	41	theme	sandy	216:220	arg1	soil					222:225	sandy soil	216:225	sandy soil near the Davis Station in Antarctica	216:262	A novel, alkaliphilic, psychrotolerant, facultative anaerobe, designated CP1T, was isolated from sandy soil near the Davis Station in Antarctica.
29616891	5	42	theme	sequence	678:685	arg1	similarity					687:696	97.4 and 97.1 % 16S rRNA gene sequence similarity	648:696	97.4 and 97.1 % 16S rRNA gene sequence similarity	648:696	16S rRNA gene and multilocus (pheS, rpoA, and atpA) sequence analysis revealed Carnobacterium mobile DSM 4848T and Carnobacterium iners LMG 26642T as the closest relatives (97.4 and 97.1 % 16S rRNA gene sequence similarity, respectively).
29616891	4	43	dep	5.0 	457:460	arg1	to					454:455	to	454:455	to	454:455	Strain CP1T was able to grow at temperatures between 4 and 36 °C, pH 6.0-9.5, and in the presence of up to 5.0 % (w/v) NaCl.
29616891	6	44	theme	38.1 mol	742:749	arg1	G+C content					726:736	The genomic G+C content	714:736	The genomic G+C content	714:736	The genomic G+C content was 38.1 mol%, and DNA-DNA hybridization with DSM 4848T revealed 32.4±3.4 % similarity.
29616891	6	44	theme	38.1 mol	742:749	arg1	%					750:750	38.1 mol%	742:750	38.1 mol%	742:750	The genomic G+C content was 38.1 mol%, and DNA-DNA hybridization with DSM 4848T revealed 32.4±3.4 % similarity.
29616891	5	45	theme	Carnobacterium	590:603	arg1	26642T					615:620	Carnobacterium iners LMG 26642T	590:620	Carnobacterium iners LMG 26642T	590:620	16S rRNA gene and multilocus (pheS, rpoA, and atpA) sequence analysis revealed Carnobacterium mobile DSM 4848T and Carnobacterium iners LMG 26642T as the closest relatives (97.4 and 97.1 % 16S rRNA gene sequence similarity, respectively).
29616891	5	45	theme	Carnobacterium	590:603	arg1	4848T					580:584	Carnobacterium mobile DSM 4848T	554:584	Carnobacterium mobile DSM 4848T	554:584	16S rRNA gene and multilocus (pheS, rpoA, and atpA) sequence analysis revealed Carnobacterium mobile DSM 4848T and Carnobacterium iners LMG 26642T as the closest relatives (97.4 and 97.1 % 16S rRNA gene sequence similarity, respectively).
29616891	5	45	theme	Carnobacterium	590:603	arg1	relatives					637:645	the closest relatives	625:645	the closest relatives (97.4 and 97.1 % 16S rRNA gene sequence similarity, respectively)	625:711	16S rRNA gene and multilocus (pheS, rpoA, and atpA) sequence analysis revealed Carnobacterium mobile DSM 4848T and Carnobacterium iners LMG 26642T as the closest relatives (97.4 and 97.1 % 16S rRNA gene sequence similarity, respectively).
29616891	8	46	contain	contained	901:909	arg1	wall					896:899	The cell wall	887:899	The cell wall	887:899	The cell wall contained meso-diaminopimelic acid and was of peptidoglycan type A1γ.
29616891	8	46	contain	contained	901:909	arg2	acid					931:934	meso-diaminopimelic acid	911:934	meso-diaminopimelic acid	911:934	The cell wall contained meso-diaminopimelic acid and was of peptidoglycan type A1γ.
29616891	8	47	theme	type	961:964	arg1	A1γ					966:968	peptidoglycan type A1γ	947:968	peptidoglycan type A1γ	947:968	The cell wall contained meso-diaminopimelic acid and was of peptidoglycan type A1γ.
29616891	4	48	theme	NaCl	469:472	arg1	presence					439:446	the presence	435:446	the presence of up to 5.0 % (w/v) NaCl	435:472	Strain CP1T was able to grow at temperatures between 4 and 36 °C, pH 6.0-9.5, and in the presence of up to 5.0 % (w/v) NaCl.
29616891	2	49	theme	novel	121:125	arg1	anaerobe					171:178	A novel, alkaliphilic, psychrotolerant, facultative anaerobe	119:178	A novel, alkaliphilic, psychrotolerant, facultative anaerobe	119:178	A novel, alkaliphilic, psychrotolerant, facultative anaerobe, designated CP1T, was isolated from sandy soil near the Davis Station in Antarctica.
29616891	8	50	theme	meso-diaminopimelic	911:929	arg1	acid					931:934	meso-diaminopimelic acid	911:934	meso-diaminopimelic acid	911:934	The cell wall contained meso-diaminopimelic acid and was of peptidoglycan type A1γ.
29616891	4	51	theme	Strain	350:355	arg1	CP1T					357:360	Strain CP1T	350:360	Strain CP1T	350:360	Strain CP1T was able to grow at temperatures between 4 and 36 °C, pH 6.0-9.5, and in the presence of up to 5.0 % (w/v) NaCl.
29616891	1	52	from	soil	99:102	arg1	Antarctica					107:116	Antarctica	107:116	Antarctica	107:116	nov., a psychrotolerant, alkaliphilic bacterium isolated from sandy soil in Antarctica.
29616891	3	53	theme	short-rod-shaped	269:284	arg1	cells					286:290	The short-rod-shaped cells	265:290	The short-rod-shaped cells	265:290	The short-rod-shaped cells displayed Gram-positive staining and did not form spores.
29616891	9	54	theme	antarcticum	1139:1149	arg1	sp					1151:1152	antarcticum sp	1139:1152	the name Carnobacterium antarcticum sp	1115:1152	Based on physiological, genotypic and biochemical characteristics, strain CP1T represents a novel species of the genus Carnobacterium for which the name Carnobacterium antarcticum sp.
29616891	9	55	theme	biochemical	1009:1019	arg1	characteristics					1021:1035	physiological, genotypic and biochemical characteristics	980:1035	physiological, genotypic and biochemical characteristics	980:1035	Based on physiological, genotypic and biochemical characteristics, strain CP1T represents a novel species of the genus Carnobacterium for which the name Carnobacterium antarcticum sp.
29616891	9	56	theme	genotypic	995:1003	arg1	characteristics					1021:1035	physiological, genotypic and biochemical characteristics	980:1035	physiological, genotypic and biochemical characteristics	980:1035	Based on physiological, genotypic and biochemical characteristics, strain CP1T represents a novel species of the genus Carnobacterium for which the name Carnobacterium antarcticum sp.
29616891	5	57	theme	iners	605:609	arg1	26642T					615:620	Carnobacterium iners LMG 26642T	590:620	Carnobacterium iners LMG 26642T	590:620	16S rRNA gene and multilocus (pheS, rpoA, and atpA) sequence analysis revealed Carnobacterium mobile DSM 4848T and Carnobacterium iners LMG 26642T as the closest relatives (97.4 and 97.1 % 16S rRNA gene sequence similarity, respectively).
29616891	5	57	theme	iners	605:609	arg1	4848T					580:584	Carnobacterium mobile DSM 4848T	554:584	Carnobacterium mobile DSM 4848T	554:584	16S rRNA gene and multilocus (pheS, rpoA, and atpA) sequence analysis revealed Carnobacterium mobile DSM 4848T and Carnobacterium iners LMG 26642T as the closest relatives (97.4 and 97.1 % 16S rRNA gene sequence similarity, respectively).
29616891	5	57	theme	iners	605:609	arg1	relatives					637:645	the closest relatives	625:645	the closest relatives (97.4 and 97.1 % 16S rRNA gene sequence similarity, respectively)	625:711	16S rRNA gene and multilocus (pheS, rpoA, and atpA) sequence analysis revealed Carnobacterium mobile DSM 4848T and Carnobacterium iners LMG 26642T as the closest relatives (97.4 and 97.1 % 16S rRNA gene sequence similarity, respectively).
29616891	6	58	theme	genomic	718:724	arg1	G+C content					726:736	The genomic G+C content	714:736	The genomic G+C content	714:736	The genomic G+C content was 38.1 mol%, and DNA-DNA hybridization with DSM 4848T revealed 32.4±3.4 % similarity.
29616891	6	58	theme	genomic	718:724	arg1	%					750:750	38.1 mol%	742:750	38.1 mol%	742:750	The genomic G+C content was 38.1 mol%, and DNA-DNA hybridization with DSM 4848T revealed 32.4±3.4 % similarity.
29616891	2	59	theme	Davis	236:240	arg1	Station					242:248	the Davis Station	232:248	the Davis Station in Antarctica	232:262	A novel, alkaliphilic, psychrotolerant, facultative anaerobe, designated CP1T, was isolated from sandy soil near the Davis Station in Antarctica.
29616891	6	60	with	hybridization	765:777	arg1	4848T					788:792	DSM 4848T	784:792	DSM 4848T	784:792	The genomic G+C content was 38.1 mol%, and DNA-DNA hybridization with DSM 4848T revealed 32.4±3.4 % similarity.
29616891	9	61	dep	Carnobacterium	1124:1137	arg1	sp					1151:1152	antarcticum sp	1139:1152	the name Carnobacterium antarcticum sp	1115:1152	Based on physiological, genotypic and biochemical characteristics, strain CP1T represents a novel species of the genus Carnobacterium for which the name Carnobacterium antarcticum sp.
29616891	4	62	dep	NaCl	469:472	arg1	%					461:461	%	461:461	%	461:461	Strain CP1T was able to grow at temperatures between 4 and 36 °C, pH 6.0-9.5, and in the presence of up to 5.0 % (w/v) NaCl.
29616891	1	63	theme	psychrotolerant	39:53	arg1	bacterium					69:77	a psychrotolerant, alkaliphilic bacterium	37:77	a psychrotolerant, alkaliphilic bacterium	37:77	nov., a psychrotolerant, alkaliphilic bacterium isolated from sandy soil in Antarctica.
29616891	1	63	theme	psychrotolerant	39:53	arg1	nov.					31:34	nov.	31:34	nov.	31:34	nov., a psychrotolerant, alkaliphilic bacterium isolated from sandy soil in Antarctica.
29616891	9	64	theme	strain	1038:1043	arg1	CP1T					1045:1048	strain CP1T	1038:1048	strain CP1T	1038:1048	Based on physiological, genotypic and biochemical characteristics, strain CP1T represents a novel species of the genus Carnobacterium for which the name Carnobacterium antarcticum sp.
29616891	11	65	theme	=DSM	1198:1201	arg1	CP1T					1192:1195	CP1T	1192:1195	CP1T (=DSM 103363T=CGMCC 1.15643T)	1192:1225	The type strain is CP1T (=DSM 103363T=CGMCC 1.15643T).
29616891	11	65	theme	=DSM	1198:1201	arg1	1.15643T					1217:1224	=DSM 103363T=CGMCC 1.15643T	1198:1224	=DSM 103363T=CGMCC 1.15643T	1198:1224	The type strain is CP1T (=DSM 103363T=CGMCC 1.15643T).
29401409	0	0	theme	Chinese	67:73	arg1	landrace					81:88	Chinese wheat landrace	67:88	Chinese wheat landrace	67:88	Alternative splicing results in a lack of starch synthase IIa-D in Chinese wheat landrace.
29401409	3	1	located	found	318:322	arg1	region					340:345	the junction region	327:345	the junction region of the 7th intron and 8th exon, leading to deletion of the intron-exon junction recognition site AG	327:445	An 18-bp deletion and 9-bp variation were found at the junction region of the 7th intron and 8th exon, leading to deletion of the intron-exon junction recognition site AG when aligned the 8261-bp DNA sequence of TaSSIIa-D in Pushanbamai with that of Chinese Spring.
29401409	3	1	located	found	318:322	arg2	variation					303:311	9-bp variation	298:311	9-bp variation	298:311	An 18-bp deletion and 9-bp variation were found at the junction region of the 7th intron and 8th exon, leading to deletion of the intron-exon junction recognition site AG when aligned the 8261-bp DNA sequence of TaSSIIa-D in Pushanbamai with that of Chinese Spring.
29401409	3	1	located	found	318:322	arg2	deletion					285:292	An 18-bp deletion	276:292	An 18-bp deletion	276:292	An 18-bp deletion and 9-bp variation were found at the junction region of the 7th intron and 8th exon, leading to deletion of the intron-exon junction recognition site AG when aligned the 8261-bp DNA sequence of TaSSIIa-D in Pushanbamai with that of Chinese Spring.
29401409	7	2	theme	field	1007:1011	arg1	conditions					1013:1022	field conditions	1007:1022	field conditions	1007:1022	The amylose content in Pushanbamai was significantly higher than that in all control lines under field conditions, which suggested that deletion of SGP-D1 has an efficient impact on amylose content.
29401409	3	3	theme	AG	444:445	arg1	deletion					390:397	deletion	390:397	deletion of the intron-exon junction recognition site AG	390:445	An 18-bp deletion and 9-bp variation were found at the junction region of the 7th intron and 8th exon, leading to deletion of the intron-exon junction recognition site AG when aligned the 8261-bp DNA sequence of TaSSIIa-D in Pushanbamai with that of Chinese Spring.
29401409	6	4	theme	type	833:836	arg1	cDNA					841:844	the type IV cDNA	829:844	the type IV cDNA	829:844	In the type IV cDNA, a 97-bp sequence remains undeleted in the end of the 5th exon.
29401409	1	5	theme	Chinese	141:147	arg1	landraces					155:163	368 Chinese wheat landraces	137:163	368 Chinese wheat landraces	137:163	We evaluated the SGP-1 protein composition of 368 Chinese wheat landraces using SDS-PAGE.
29401409	3	6	from	sequence	476:483	arg1	Pushanbamai					501:511	Pushanbamai	501:511	Pushanbamai	501:511	An 18-bp deletion and 9-bp variation were found at the junction region of the 7th intron and 8th exon, leading to deletion of the intron-exon junction recognition site AG when aligned the 8261-bp DNA sequence of TaSSIIa-D in Pushanbamai with that of Chinese Spring.
29401409	5	7	theme	type	671:674	arg1	cDNAs					679:683	nine type II cDNAs	666:683	nine type II cDNAs with a 16-bp deletion in the 8th exon	666:721	Among these, nine type II cDNAs with a 16-bp deletion in the 8th exon were detected, indicating that the major transcriptional pattern of TaSSIIa in Pushanbamai is type II.
29401409	7	8	theme	amylose	1092:1098	arg1	content					1100:1106	amylose content	1092:1106	amylose content	1092:1106	The amylose content in Pushanbamai was significantly higher than that in all control lines under field conditions, which suggested that deletion of SGP-D1 has an efficient impact on amylose content.
29401409	8	9	theme	TaSSIIa-D	1240:1248	arg1	variants					1228:1235	these natural variants	1214:1235	these natural variants of TaSSIIa-D	1214:1248	As the TaSSIIa gene plays an important role in regulating the content of amylose, it is anticipated that these natural variants of TaSSIIa-D will provide useful resources for quality improvement in wheat.
29401409	0	10	theme	wheat	75:79	arg1	landrace					81:88	Chinese wheat landrace	67:88	Chinese wheat landrace	67:88	Alternative splicing results in a lack of starch synthase IIa-D in Chinese wheat landrace.
29401409	3	11	theme	18-bp	279:283	arg1	deletion					285:292	An 18-bp deletion	276:292	An 18-bp deletion	276:292	An 18-bp deletion and 9-bp variation were found at the junction region of the 7th intron and 8th exon, leading to deletion of the intron-exon junction recognition site AG when aligned the 8261-bp DNA sequence of TaSSIIa-D in Pushanbamai with that of Chinese Spring.
29401409	3	12	theme	junction	418:425	arg1	site					439:442	the intron-exon junction recognition site	402:442	the intron-exon junction recognition site AG	402:445	An 18-bp deletion and 9-bp variation were found at the junction region of the 7th intron and 8th exon, leading to deletion of the intron-exon junction recognition site AG when aligned the 8261-bp DNA sequence of TaSSIIa-D in Pushanbamai with that of Chinese Spring.
29401409	3	13	theme	8261-bp	464:470	arg1	sequence					476:483	the 8261-bp DNA sequence	460:483	the 8261-bp DNA sequence of TaSSIIa-D in Pushanbamai	460:511	An 18-bp deletion and 9-bp variation were found at the junction region of the 7th intron and 8th exon, leading to deletion of the intron-exon junction recognition site AG when aligned the 8261-bp DNA sequence of TaSSIIa-D in Pushanbamai with that of Chinese Spring.
29401409	1	14	theme	wheat	149:153	arg1	landraces					155:163	368 Chinese wheat landraces	137:163	368 Chinese wheat landraces	137:163	We evaluated the SGP-1 protein composition of 368 Chinese wheat landraces using SDS-PAGE.
29401409	7	15	theme	efficient	1072:1080	arg1	impact					1082:1087	an efficient impact	1069:1087	an efficient impact on amylose content	1069:1106	The amylose content in Pushanbamai was significantly higher than that in all control lines under field conditions, which suggested that deletion of SGP-D1 has an efficient impact on amylose content.
29401409	0	16	from	lack	34:37	arg1	landrace					81:88	Chinese wheat landrace	67:88	Chinese wheat landrace	67:88	Alternative splicing results in a lack of starch synthase IIa-D in Chinese wheat landrace.
29401409	7	17	theme	control	987:993	arg1	lines					995:999	all control lines	983:999	all control lines	983:999	The amylose content in Pushanbamai was significantly higher than that in all control lines under field conditions, which suggested that deletion of SGP-D1 has an efficient impact on amylose content.
29401409	3	18	theme	DNA	472:474	arg1	sequence					476:483	the 8261-bp DNA sequence	460:483	the 8261-bp DNA sequence of TaSSIIa-D in Pushanbamai	460:511	An 18-bp deletion and 9-bp variation were found at the junction region of the 7th intron and 8th exon, leading to deletion of the intron-exon junction recognition site AG when aligned the 8261-bp DNA sequence of TaSSIIa-D in Pushanbamai with that of Chinese Spring.
29401409	8	19	theme	useful	1263:1268	arg1	resources					1270:1278	useful resources	1263:1278	useful resources for quality improvement in wheat	1263:1311	As the TaSSIIa gene plays an important role in regulating the content of amylose, it is anticipated that these natural variants of TaSSIIa-D will provide useful resources for quality improvement in wheat.
29401409	1	20	theme	landraces	155:163	arg1	composition					122:132	the SGP-1 protein composition	104:132	the SGP-1 protein composition of 368 Chinese wheat landraces	104:163	We evaluated the SGP-1 protein composition of 368 Chinese wheat landraces using SDS-PAGE.
29401409	7	21	theme	SGP-D1	1058:1063	arg1	deletion					1046:1053	deletion	1046:1053	deletion of SGP-D1	1046:1063	The amylose content in Pushanbamai was significantly higher than that in all control lines under field conditions, which suggested that deletion of SGP-D1 has an efficient impact on amylose content.
29401409	3	22	dep	intron	358:363	arg1	the					350:352	the	350:352	the	350:352	An 18-bp deletion and 9-bp variation were found at the junction region of the 7th intron and 8th exon, leading to deletion of the intron-exon junction recognition site AG when aligned the 8261-bp DNA sequence of TaSSIIa-D in Pushanbamai with that of Chinese Spring.
29401409	3	23	theme	8th	369:371	arg1	exon					373:376	8th exon	369:376	8th exon	369:376	An 18-bp deletion and 9-bp variation were found at the junction region of the 7th intron and 8th exon, leading to deletion of the intron-exon junction recognition site AG when aligned the 8261-bp DNA sequence of TaSSIIa-D in Pushanbamai with that of Chinese Spring.
29401409	0	24	theme	Alternative	0:10	arg1	splicing					12:19	Alternative splicing	0:19	Alternative splicing	0:19	Alternative splicing results in a lack of starch synthase IIa-D in Chinese wheat landrace.
29401409	8	25	theme	TaSSIIa	1116:1122	arg1	gene					1124:1127	the TaSSIIa gene	1112:1127	the TaSSIIa gene	1112:1127	As the TaSSIIa gene plays an important role in regulating the content of amylose, it is anticipated that these natural variants of TaSSIIa-D will provide useful resources for quality improvement in wheat.
29401409	7	26	contain	has	1065:1067	arg1	deletion					1046:1053	deletion	1046:1053	deletion of SGP-D1	1046:1063	The amylose content in Pushanbamai was significantly higher than that in all control lines under field conditions, which suggested that deletion of SGP-D1 has an efficient impact on amylose content.
29401409	7	26	contain	has	1065:1067	arg2	impact					1082:1087	an efficient impact	1069:1087	an efficient impact on amylose content	1069:1106	The amylose content in Pushanbamai was significantly higher than that in all control lines under field conditions, which suggested that deletion of SGP-D1 has an efficient impact on amylose content.
29401409	5	27	from	deletion	698:705	arg1	exon					718:721	the 8th exon	710:721	the 8th exon	710:721	Among these, nine type II cDNAs with a 16-bp deletion in the 8th exon were detected, indicating that the major transcriptional pattern of TaSSIIa in Pushanbamai is type II.
29401409	3	28	theme	exon	373:376	arg1	region					340:345	the junction region	327:345	the junction region of the 7th intron and 8th exon, leading to deletion of the intron-exon junction recognition site AG	327:445	An 18-bp deletion and 9-bp variation were found at the junction region of the 7th intron and 8th exon, leading to deletion of the intron-exon junction recognition site AG when aligned the 8261-bp DNA sequence of TaSSIIa-D in Pushanbamai with that of Chinese Spring.
29401409	5	29	with	cDNAs	679:683	arg1	deletion					698:705	a 16-bp deletion	690:705	a 16-bp deletion in the 8th exon	690:721	Among these, nine type II cDNAs with a 16-bp deletion in the 8th exon were detected, indicating that the major transcriptional pattern of TaSSIIa in Pushanbamai is type II.
29401409	3	30	theme	TaSSIIa-D	488:496	arg1	sequence					476:483	the 8261-bp DNA sequence	460:483	the 8261-bp DNA sequence of TaSSIIa-D in Pushanbamai	460:511	An 18-bp deletion and 9-bp variation were found at the junction region of the 7th intron and 8th exon, leading to deletion of the intron-exon junction recognition site AG when aligned the 8261-bp DNA sequence of TaSSIIa-D in Pushanbamai with that of Chinese Spring.
29401409	4	31	with	types	552:556	arg1	isoforms					575:582	mis-spliced isoforms	563:582	mis-spliced isoforms	563:582	Four cDNA types with mis-spliced isoforms were subsequently detected through amplification of TaSSIIa-D cDNAs.
29401409	4	32	theme	mis-spliced	563:573	arg1	isoforms					575:582	mis-spliced isoforms	563:582	mis-spliced isoforms	563:582	Four cDNA types with mis-spliced isoforms were subsequently detected through amplification of TaSSIIa-D cDNAs.
29401409	3	33	theme	junction	331:338	arg1	region					340:345	the junction region	327:345	the junction region of the 7th intron and 8th exon, leading to deletion of the intron-exon junction recognition site AG	327:445	An 18-bp deletion and 9-bp variation were found at the junction region of the 7th intron and 8th exon, leading to deletion of the intron-exon junction recognition site AG when aligned the 8261-bp DNA sequence of TaSSIIa-D in Pushanbamai with that of Chinese Spring.
29401409	8	34	theme	natural	1220:1226	arg1	variants					1228:1235	these natural variants	1214:1235	these natural variants of TaSSIIa-D	1214:1248	As the TaSSIIa gene plays an important role in regulating the content of amylose, it is anticipated that these natural variants of TaSSIIa-D will provide useful resources for quality improvement in wheat.
29401409	4	35	theme	cDNAs	646:650	arg1	amplification					619:631	amplification	619:631	amplification of TaSSIIa-D cDNAs	619:650	Four cDNA types with mis-spliced isoforms were subsequently detected through amplification of TaSSIIa-D cDNAs.
29401409	8	36	from	improvement	1292:1302	arg1	wheat					1307:1311	wheat	1307:1311	wheat	1307:1311	As the TaSSIIa gene plays an important role in regulating the content of amylose, it is anticipated that these natural variants of TaSSIIa-D will provide useful resources for quality improvement in wheat.
29401409	5	37	theme	major	758:762	arg1	pattern					780:786	the major transcriptional pattern	754:786	the major transcriptional pattern of TaSSIIa in Pushanbamai	754:812	Among these, nine type II cDNAs with a 16-bp deletion in the 8th exon were detected, indicating that the major transcriptional pattern of TaSSIIa in Pushanbamai is type II.
29401409	5	37	theme	major	758:762	arg1	type					817:820	type II	817:823	type II	817:823	Among these, nine type II cDNAs with a 16-bp deletion in the 8th exon were detected, indicating that the major transcriptional pattern of TaSSIIa in Pushanbamai is type II.
29401409	3	38	theme	intron	358:363	arg1	region					340:345	the junction region	327:345	the junction region of the 7th intron and 8th exon, leading to deletion of the intron-exon junction recognition site AG	327:445	An 18-bp deletion and 9-bp variation were found at the junction region of the 7th intron and 8th exon, leading to deletion of the intron-exon junction recognition site AG when aligned the 8261-bp DNA sequence of TaSSIIa-D in Pushanbamai with that of Chinese Spring.
29401409	3	39	theme	Chinese	526:532	arg1	Spring					534:539	Chinese Spring	526:539	Chinese Spring	526:539	An 18-bp deletion and 9-bp variation were found at the junction region of the 7th intron and 8th exon, leading to deletion of the intron-exon junction recognition site AG when aligned the 8261-bp DNA sequence of TaSSIIa-D in Pushanbamai with that of Chinese Spring.
29401409	5	40	theme	transcriptional	764:778	arg1	pattern					780:786	the major transcriptional pattern	754:786	the major transcriptional pattern of TaSSIIa in Pushanbamai	754:812	Among these, nine type II cDNAs with a 16-bp deletion in the 8th exon were detected, indicating that the major transcriptional pattern of TaSSIIa in Pushanbamai is type II.
29401409	5	40	theme	transcriptional	764:778	arg1	type					817:820	type II	817:823	type II	817:823	Among these, nine type II cDNAs with a 16-bp deletion in the 8th exon were detected, indicating that the major transcriptional pattern of TaSSIIa in Pushanbamai is type II.
29401409	2	41	dep	accessions	226:235	arg1	accessions					226:235	three accessions	220:235	three accessions (Xiaoqingmang, Pushanbamai, and P119)	220:273	The SGP-D1 null type was identified in three accessions (Xiaoqingmang, Pushanbamai, and P119).
29401409	2	41	dep	accessions	226:235	arg1	Pushanbamai					252:262	Pushanbamai	252:262	Pushanbamai	252:262	The SGP-D1 null type was identified in three accessions (Xiaoqingmang, Pushanbamai, and P119).
29401409	2	41	dep	accessions	226:235	arg1	P119					269:272	P119	269:272	P119	269:272	The SGP-D1 null type was identified in three accessions (Xiaoqingmang, Pushanbamai, and P119).
29401409	2	41	dep	accessions	226:235	arg1	Xiaoqingmang					238:249	Xiaoqingmang	238:249	Xiaoqingmang	238:249	The SGP-D1 null type was identified in three accessions (Xiaoqingmang, Pushanbamai, and P119).
29401409	6	42	theme	97-bp	849:853	arg1	sequence					855:862	a 97-bp sequence	847:862	a 97-bp sequence	847:862	In the type IV cDNA, a 97-bp sequence remains undeleted in the end of the 5th exon.
29401409	5	43	theme	8th	714:716	arg1	exon					718:721	the 8th exon	710:721	the 8th exon	710:721	Among these, nine type II cDNAs with a 16-bp deletion in the 8th exon were detected, indicating that the major transcriptional pattern of TaSSIIa in Pushanbamai is type II.
29401409	5	44	theme	II	676:677	arg1	cDNAs					679:683	nine type II cDNAs	666:683	nine type II cDNAs with a 16-bp deletion in the 8th exon	666:721	Among these, nine type II cDNAs with a 16-bp deletion in the 8th exon were detected, indicating that the major transcriptional pattern of TaSSIIa in Pushanbamai is type II.
29401409	0	45	theme	starch	42:47	arg1	IIa-D					58:62	starch synthase IIa-D	42:62	starch synthase IIa-D	42:62	Alternative splicing results in a lack of starch synthase IIa-D in Chinese wheat landrace.
29401409	7	46	from	content	922:928	arg1	Pushanbamai					933:943	Pushanbamai	933:943	Pushanbamai	933:943	The amylose content in Pushanbamai was significantly higher than that in all control lines under field conditions, which suggested that deletion of SGP-D1 has an efficient impact on amylose content.
29401409	3	47	theme	9-bp	298:301	arg1	variation					303:311	9-bp variation	298:311	9-bp variation	298:311	An 18-bp deletion and 9-bp variation were found at the junction region of the 7th intron and 8th exon, leading to deletion of the intron-exon junction recognition site AG when aligned the 8261-bp DNA sequence of TaSSIIa-D in Pushanbamai with that of Chinese Spring.
29401409	5	48	from	pattern	780:786	arg1	Pushanbamai					802:812	Pushanbamai	802:812	Pushanbamai	802:812	Among these, nine type II cDNAs with a 16-bp deletion in the 8th exon were detected, indicating that the major transcriptional pattern of TaSSIIa in Pushanbamai is type II.
29401409	3	49	theme	recognition	427:437	arg1	site					439:442	the intron-exon junction recognition site	402:442	the intron-exon junction recognition site AG	402:445	An 18-bp deletion and 9-bp variation were found at the junction region of the 7th intron and 8th exon, leading to deletion of the intron-exon junction recognition site AG when aligned the 8261-bp DNA sequence of TaSSIIa-D in Pushanbamai with that of Chinese Spring.
29401409	3	50	theme	7th	354:356	arg1	intron					358:363	7th intron	354:363	7th intron	354:363	An 18-bp deletion and 9-bp variation were found at the junction region of the 7th intron and 8th exon, leading to deletion of the intron-exon junction recognition site AG when aligned the 8261-bp DNA sequence of TaSSIIa-D in Pushanbamai with that of Chinese Spring.
29401409	2	51	theme	null	192:195	arg1	type					197:200	The SGP-D1 null type	181:200	The SGP-D1 null type	181:200	The SGP-D1 null type was identified in three accessions (Xiaoqingmang, Pushanbamai, and P119).
29401409	3	52	theme	site	439:442	arg1	AG					444:445	the intron-exon junction recognition site AG	402:445	the intron-exon junction recognition site AG	402:445	An 18-bp deletion and 9-bp variation were found at the junction region of the 7th intron and 8th exon, leading to deletion of the intron-exon junction recognition site AG when aligned the 8261-bp DNA sequence of TaSSIIa-D in Pushanbamai with that of Chinese Spring.
29401409	7	53	theme	amylose	914:920	arg1	higher					963:968	higher	963:968	higher	963:968	The amylose content in Pushanbamai was significantly higher than that in all control lines under field conditions, which suggested that deletion of SGP-D1 has an efficient impact on amylose content.
29401409	7	53	theme	amylose	914:920	arg1	content					922:928	The amylose content	910:928	The amylose content in Pushanbamai	910:943	The amylose content in Pushanbamai was significantly higher than that in all control lines under field conditions, which suggested that deletion of SGP-D1 has an efficient impact on amylose content.
29401409	2	54	theme	SGP-D1	185:190	arg1	type					197:200	The SGP-D1 null type	181:200	The SGP-D1 null type	181:200	The SGP-D1 null type was identified in three accessions (Xiaoqingmang, Pushanbamai, and P119).
29401409	5	55	theme	16-bp	692:696	arg1	deletion					698:705	a 16-bp deletion	690:705	a 16-bp deletion in the 8th exon	690:721	Among these, nine type II cDNAs with a 16-bp deletion in the 8th exon were detected, indicating that the major transcriptional pattern of TaSSIIa in Pushanbamai is type II.
29401409	1	56	theme	SGP-1	108:112	arg1	composition					122:132	the SGP-1 protein composition	104:132	the SGP-1 protein composition of 368 Chinese wheat landraces	104:163	We evaluated the SGP-1 protein composition of 368 Chinese wheat landraces using SDS-PAGE.
29401409	7	57	from	impact	1082:1087	arg1	content					1100:1106	amylose content	1092:1106	amylose content	1092:1106	The amylose content in Pushanbamai was significantly higher than that in all control lines under field conditions, which suggested that deletion of SGP-D1 has an efficient impact on amylose content.
29401409	8	58	theme	quality	1284:1290	arg1	improvement					1292:1302	quality improvement	1284:1302	quality improvement in wheat	1284:1311	As the TaSSIIa gene plays an important role in regulating the content of amylose, it is anticipated that these natural variants of TaSSIIa-D will provide useful resources for quality improvement in wheat.
29401409	1	59	theme	protein	114:120	arg1	composition					122:132	the SGP-1 protein composition	104:132	the SGP-1 protein composition of 368 Chinese wheat landraces	104:163	We evaluated the SGP-1 protein composition of 368 Chinese wheat landraces using SDS-PAGE.
29401409	3	60	theme	intron-exon	406:416	arg1	site					439:442	the intron-exon junction recognition site	402:442	the intron-exon junction recognition site AG	402:445	An 18-bp deletion and 9-bp variation were found at the junction region of the 7th intron and 8th exon, leading to deletion of the intron-exon junction recognition site AG when aligned the 8261-bp DNA sequence of TaSSIIa-D in Pushanbamai with that of Chinese Spring.
29401409	0	61	theme	IIa-D	58:62	arg1	lack					34:37	a lack	32:37	a lack of starch synthase IIa-D in Chinese wheat landrace	32:88	Alternative splicing results in a lack of starch synthase IIa-D in Chinese wheat landrace.
29401409	8	62	theme	important	1138:1146	arg1	role					1148:1151	an important role	1135:1151	an important role	1135:1151	As the TaSSIIa gene plays an important role in regulating the content of amylose, it is anticipated that these natural variants of TaSSIIa-D will provide useful resources for quality improvement in wheat.
29401409	4	63	theme	TaSSIIa-D	636:644	arg1	cDNAs					646:650	TaSSIIa-D cDNAs	636:650	TaSSIIa-D cDNAs	636:650	Four cDNA types with mis-spliced isoforms were subsequently detected through amplification of TaSSIIa-D cDNAs.
29401409	5	64	theme	TaSSIIa	791:797	arg1	pattern					780:786	the major transcriptional pattern	754:786	the major transcriptional pattern of TaSSIIa in Pushanbamai	754:812	Among these, nine type II cDNAs with a 16-bp deletion in the 8th exon were detected, indicating that the major transcriptional pattern of TaSSIIa in Pushanbamai is type II.
29401409	5	64	theme	TaSSIIa	791:797	arg1	type					817:820	type II	817:823	type II	817:823	Among these, nine type II cDNAs with a 16-bp deletion in the 8th exon were detected, indicating that the major transcriptional pattern of TaSSIIa in Pushanbamai is type II.
29401409	6	65	theme	exon	904:907	arg1	end					889:891	the end	885:891	the end of the 5th exon	885:907	In the type IV cDNA, a 97-bp sequence remains undeleted in the end of the 5th exon.
29401409	0	66	theme	synthase	49:56	arg1	IIa-D					58:62	starch synthase IIa-D	42:62	starch synthase IIa-D	42:62	Alternative splicing results in a lack of starch synthase IIa-D in Chinese wheat landrace.
29401409	6	67	theme	IV	838:839	arg1	cDNA					841:844	the type IV cDNA	829:844	the type IV cDNA	829:844	In the type IV cDNA, a 97-bp sequence remains undeleted in the end of the 5th exon.
29401409	6	68	theme	5th	900:902	arg1	exon					904:907	the 5th exon	896:907	the 5th exon	896:907	In the type IV cDNA, a 97-bp sequence remains undeleted in the end of the 5th exon.
29401409	8	69	theme	amylose	1182:1188	arg1	content					1171:1177	the content	1167:1177	the content of amylose	1167:1188	As the TaSSIIa gene plays an important role in regulating the content of amylose, it is anticipated that these natural variants of TaSSIIa-D will provide useful resources for quality improvement in wheat.
29401409	4	70	theme	cDNA	547:550	arg1	types					552:556	Four cDNA types	542:556	Four cDNA types with mis-spliced isoforms	542:582	Four cDNA types with mis-spliced isoforms were subsequently detected through amplification of TaSSIIa-D cDNAs.
30940593	0	0	theme	carbon	108:113	arg1	scaffold					150:157	poly 3-hydroxybutyrate-chitosan-multiwalled carbon nanotube/silk nano-micro composite scaffold	64:157	poly 3-hydroxybutyrate-chitosan-multiwalled carbon nanotube/silk nano-micro composite scaffold for cartilage tissue engineering applications	64:203	Evaluation of physical, mechanical and biological properties of poly 3-hydroxybutyrate-chitosan-multiwalled carbon nanotube/silk nano-micro composite scaffold for cartilage tissue engineering applications.
30940593	3	1	theme	tensile	742:748	arg1	strength					750:757	tensile strength	742:757	tensile strength	742:757	Consuming MWNTs lead to a decrease in fiber diameter, while an increase in specific surface area, tensile strength and bioactivity properties.
30940593	1	2	theme	Nano-micro	206:215	arg1	scaffolds					217:225	Nano-micro scaffolds	206:225	Nano-micro scaffolds	206:225	Nano-micro scaffolds are developed for long-term healing tissue engineering like cartilage.
30940593	0	3	theme	3-hydroxybutyrate-chitosan-multiwalled	69:106	arg1	scaffold					150:157	poly 3-hydroxybutyrate-chitosan-multiwalled carbon nanotube/silk nano-micro composite scaffold	64:157	poly 3-hydroxybutyrate-chitosan-multiwalled carbon nanotube/silk nano-micro composite scaffold for cartilage tissue engineering applications	64:203	Evaluation of physical, mechanical and biological properties of poly 3-hydroxybutyrate-chitosan-multiwalled carbon nanotube/silk nano-micro composite scaffold for cartilage tissue engineering applications.
30940593	3	4	theme	surface	728:734	arg1	area					736:739	specific surface area	719:739	specific surface area	719:739	Consuming MWNTs lead to a decrease in fiber diameter, while an increase in specific surface area, tensile strength and bioactivity properties.
30940593	2	5	dep	saturated	536:544	arg1	S					547:547	S	547:547	S	547:547	The poly 3-hydroxybutyrate (P3HB)-chitosan/silk and P3HB-chitosan-1 wt% multi-walled carbon nanotubes functionalized by COOH (MWNTs)/silk nano-micro scaffolds are fabricated through electrospinning the solution on a knitted silk which is saturated (S) or unsaturated (U) with P3HB as a mediator to enhance the interaction at nano/microinterface.
30940593	2	5	dep	saturated	536:544	arg1	silk					522:525	a knitted silk	512:525	a knitted silk which is saturated (S) or unsaturated (U) with P3HB as a mediator to enhance the interaction at nano/microinterface	512:641	The poly 3-hydroxybutyrate (P3HB)-chitosan/silk and P3HB-chitosan-1 wt% multi-walled carbon nanotubes functionalized by COOH (MWNTs)/silk nano-micro scaffolds are fabricated through electrospinning the solution on a knitted silk which is saturated (S) or unsaturated (U) with P3HB as a mediator to enhance the interaction at nano/microinterface.
30940593	2	5	dep	saturated	536:544	arg1	saturated					536:544	saturated	536:544	saturated	536:544	The poly 3-hydroxybutyrate (P3HB)-chitosan/silk and P3HB-chitosan-1 wt% multi-walled carbon nanotubes functionalized by COOH (MWNTs)/silk nano-micro scaffolds are fabricated through electrospinning the solution on a knitted silk which is saturated (S) or unsaturated (U) with P3HB as a mediator to enhance the interaction at nano/microinterface.
30940593	5	6	theme	tensile	942:948	arg1	strength					950:957	tensile strength	942:957	tensile strength in comparison with the pure knitted silk	942:998	The nanolayer in all scaffolds lead to an increase in tensile strength in comparison with the pure knitted silk.
30940593	7	7	theme	appropriate	1109:1119	arg1	environment					1121:1131	an appropriate environment	1106:1131	an appropriate environment for attachment and growth of chondrocytes	1106:1173	MWNTs beside the chitosan and silk provide an appropriate environment for attachment and growth of chondrocytes.
30940593	6	8	theme	degradation	1045:1055	arg1	rate					1057:1060	slower degradation rate	1038:1060	slower degradation rate	1038:1060	The scaffold containing MWNTs showed slower degradation rate.
30940593	0	9	theme	nano-micro	129:138	arg1	scaffold					150:157	poly 3-hydroxybutyrate-chitosan-multiwalled carbon nanotube/silk nano-micro composite scaffold	64:157	poly 3-hydroxybutyrate-chitosan-multiwalled carbon nanotube/silk nano-micro composite scaffold for cartilage tissue engineering applications	64:203	Evaluation of physical, mechanical and biological properties of poly 3-hydroxybutyrate-chitosan-multiwalled carbon nanotube/silk nano-micro composite scaffold for cartilage tissue engineering applications.
30940593	7	10	theme	chondrocytes	1162:1173	arg1	growth					1152:1157	growth	1152:1157	growth	1152:1157	MWNTs beside the chitosan and silk provide an appropriate environment for attachment and growth of chondrocytes.
30940593	7	10	theme	chondrocytes	1162:1173	arg1	attachment					1137:1146	attachment	1137:1146	attachment	1137:1146	MWNTs beside the chitosan and silk provide an appropriate environment for attachment and growth of chondrocytes.
30940593	5	11	from	strength	950:957	arg1	comparison					962:971	comparison	962:971	comparison with the pure knitted silk	962:998	The nanolayer in all scaffolds lead to an increase in tensile strength in comparison with the pure knitted silk.
30940593	4	12	theme	saturation	791:800	arg1	condition					802:810	The saturation condition	787:810	The saturation condition as well as MWNTs	787:827	The saturation condition as well as MWNTs leads to intensification in the hydrophilicity properties.
30940593	2	13	from	nano/microinterface	623:641	arg1	interaction					608:618	the interaction	604:618	the interaction at nano/microinterface	604:641	The poly 3-hydroxybutyrate (P3HB)-chitosan/silk and P3HB-chitosan-1 wt% multi-walled carbon nanotubes functionalized by COOH (MWNTs)/silk nano-micro scaffolds are fabricated through electrospinning the solution on a knitted silk which is saturated (S) or unsaturated (U) with P3HB as a mediator to enhance the interaction at nano/microinterface.
30940593	0	14	theme	nanotube/silk	115:127	arg1	scaffold					150:157	poly 3-hydroxybutyrate-chitosan-multiwalled carbon nanotube/silk nano-micro composite scaffold	64:157	poly 3-hydroxybutyrate-chitosan-multiwalled carbon nanotube/silk nano-micro composite scaffold for cartilage tissue engineering applications	64:203	Evaluation of physical, mechanical and biological properties of poly 3-hydroxybutyrate-chitosan-multiwalled carbon nanotube/silk nano-micro composite scaffold for cartilage tissue engineering applications.
30940593	4	15	from	intensification	838:852	arg1	properties					876:885	the hydrophilicity properties	857:885	the hydrophilicity properties	857:885	The saturation condition as well as MWNTs leads to intensification in the hydrophilicity properties.
30940593	5	16	from	increase	930:937	arg1	strength					950:957	tensile strength	942:957	tensile strength in comparison with the pure knitted silk	942:998	The nanolayer in all scaffolds lead to an increase in tensile strength in comparison with the pure knitted silk.
30940593	2	17	theme	P3HB-chitosan-1 wt	350:367	arg1	%					368:368	P3HB-chitosan-1 wt%	350:368	P3HB-chitosan-1 wt%	350:368	The poly 3-hydroxybutyrate (P3HB)-chitosan/silk and P3HB-chitosan-1 wt% multi-walled carbon nanotubes functionalized by COOH (MWNTs)/silk nano-micro scaffolds are fabricated through electrospinning the solution on a knitted silk which is saturated (S) or unsaturated (U) with P3HB as a mediator to enhance the interaction at nano/microinterface.
30940593	0	18	theme	scaffold	150:157	arg1	properties					50:59	physical, mechanical and biological properties	14:59	physical, mechanical and biological properties of poly 3-hydroxybutyrate-chitosan-multiwalled carbon nanotube/silk nano-micro composite scaffold for cartilage tissue engineering applications	14:203	Evaluation of physical, mechanical and biological properties of poly 3-hydroxybutyrate-chitosan-multiwalled carbon nanotube/silk nano-micro composite scaffold for cartilage tissue engineering applications.
30940593	8	19	theme	tissue	1263:1268	arg1	engineering					1270:1280	a long-term tissue engineering	1251:1280	a long-term tissue engineering application like cartilage	1251:1307	The P3HB-chitosan-MWNTs/silk (S) nano-microscaffold can be appropriate for a long-term tissue engineering application like cartilage.
30940593	0	20	theme	composite	140:148	arg1	scaffold					150:157	poly 3-hydroxybutyrate-chitosan-multiwalled carbon nanotube/silk nano-micro composite scaffold	64:157	poly 3-hydroxybutyrate-chitosan-multiwalled carbon nanotube/silk nano-micro composite scaffold for cartilage tissue engineering applications	64:203	Evaluation of physical, mechanical and biological properties of poly 3-hydroxybutyrate-chitosan-multiwalled carbon nanotube/silk nano-micro composite scaffold for cartilage tissue engineering applications.
30940593	2	21	theme	-chitosan/silk	331:344	arg1	nanotubes					390:398	The poly 3-hydroxybutyrate (P3HB)-chitosan/silk and P3HB-chitosan-1 wt% multi-walled carbon nanotubes	298:398	The poly 3-hydroxybutyrate (P3HB)-chitosan/silk and P3HB-chitosan-1 wt% multi-walled carbon nanotubes functionalized by COOH (MWNTs)/silk nano-micro scaffolds	298:455	The poly 3-hydroxybutyrate (P3HB)-chitosan/silk and P3HB-chitosan-1 wt% multi-walled carbon nanotubes functionalized by COOH (MWNTs)/silk nano-micro scaffolds are fabricated through electrospinning the solution on a knitted silk which is saturated (S) or unsaturated (U) with P3HB as a mediator to enhance the interaction at nano/microinterface.
30940593	3	22	from	decrease	670:677	arg1	diameter					688:695	fiber diameter	682:695	fiber diameter	682:695	Consuming MWNTs lead to a decrease in fiber diameter, while an increase in specific surface area, tensile strength and bioactivity properties.
30940593	8	23	theme	long-term	1253:1261	arg1	engineering					1270:1280	a long-term tissue engineering	1251:1280	a long-term tissue engineering application like cartilage	1251:1307	The P3HB-chitosan-MWNTs/silk (S) nano-microscaffold can be appropriate for a long-term tissue engineering application like cartilage.
30940593	5	24	theme	pure	982:985	arg1	silk					995:998	the pure knitted silk	978:998	the pure knitted silk	978:998	The nanolayer in all scaffolds lead to an increase in tensile strength in comparison with the pure knitted silk.
30940593	5	25	with	comparison	962:971	arg1	silk					995:998	the pure knitted silk	978:998	the pure knitted silk	978:998	The nanolayer in all scaffolds lead to an increase in tensile strength in comparison with the pure knitted silk.
30940593	5	26	theme	knitted	987:993	arg1	silk					995:998	the pure knitted silk	978:998	the pure knitted silk	978:998	The nanolayer in all scaffolds lead to an increase in tensile strength in comparison with the pure knitted silk.
30940593	1	27	theme	long-term	245:253	arg1	engineering					270:280	long-term healing tissue engineering	245:280	long-term healing tissue engineering like cartilage	245:295	Nano-micro scaffolds are developed for long-term healing tissue engineering like cartilage.
30940593	8	28	theme	engineering	1270:1280	arg1	application					1282:1292	a long-term tissue engineering application	1251:1292	a long-term tissue engineering application like cartilage	1251:1307	The P3HB-chitosan-MWNTs/silk (S) nano-microscaffold can be appropriate for a long-term tissue engineering application like cartilage.
30940593	3	29	dep	area	736:739	arg1	properties					775:784	properties	775:784	properties	775:784	Consuming MWNTs lead to a decrease in fiber diameter, while an increase in specific surface area, tensile strength and bioactivity properties.
30940593	0	30	theme	cartilage	163:171	arg1	applications					192:203	cartilage tissue engineering applications	163:203	cartilage tissue engineering applications	163:203	Evaluation of physical, mechanical and biological properties of poly 3-hydroxybutyrate-chitosan-multiwalled carbon nanotube/silk nano-micro composite scaffold for cartilage tissue engineering applications.
30940593	2	31	theme	carbon	383:388	arg1	nanotubes					390:398	The poly 3-hydroxybutyrate (P3HB)-chitosan/silk and P3HB-chitosan-1 wt% multi-walled carbon nanotubes	298:398	The poly 3-hydroxybutyrate (P3HB)-chitosan/silk and P3HB-chitosan-1 wt% multi-walled carbon nanotubes functionalized by COOH (MWNTs)/silk nano-micro scaffolds	298:455	The poly 3-hydroxybutyrate (P3HB)-chitosan/silk and P3HB-chitosan-1 wt% multi-walled carbon nanotubes functionalized by COOH (MWNTs)/silk nano-micro scaffolds are fabricated through electrospinning the solution on a knitted silk which is saturated (S) or unsaturated (U) with P3HB as a mediator to enhance the interaction at nano/microinterface.
30940593	1	32	theme	healing	255:261	arg1	engineering					270:280	long-term healing tissue engineering	245:280	long-term healing tissue engineering like cartilage	245:295	Nano-micro scaffolds are developed for long-term healing tissue engineering like cartilage.
30940593	0	33	theme	physical	14:21	arg1	properties					50:59	physical, mechanical and biological properties	14:59	physical, mechanical and biological properties of poly 3-hydroxybutyrate-chitosan-multiwalled carbon nanotube/silk nano-micro composite scaffold for cartilage tissue engineering applications	14:203	Evaluation of physical, mechanical and biological properties of poly 3-hydroxybutyrate-chitosan-multiwalled carbon nanotube/silk nano-micro composite scaffold for cartilage tissue engineering applications.
30940593	6	34	theme	scaffold	1005:1012	arg1	MWNTs					1025:1029	The scaffold containing MWNTs	1001:1029	The scaffold containing MWNTs	1001:1029	The scaffold containing MWNTs showed slower degradation rate.
30940593	2	35	theme	multi-walled	370:381	arg1	nanotubes					390:398	The poly 3-hydroxybutyrate (P3HB)-chitosan/silk and P3HB-chitosan-1 wt% multi-walled carbon nanotubes	298:398	The poly 3-hydroxybutyrate (P3HB)-chitosan/silk and P3HB-chitosan-1 wt% multi-walled carbon nanotubes functionalized by COOH (MWNTs)/silk nano-micro scaffolds	298:455	The poly 3-hydroxybutyrate (P3HB)-chitosan/silk and P3HB-chitosan-1 wt% multi-walled carbon nanotubes functionalized by COOH (MWNTs)/silk nano-micro scaffolds are fabricated through electrospinning the solution on a knitted silk which is saturated (S) or unsaturated (U) with P3HB as a mediator to enhance the interaction at nano/microinterface.
30940593	1	36	theme	tissue	263:268	arg1	engineering					270:280	long-term healing tissue engineering	245:280	long-term healing tissue engineering like cartilage	245:295	Nano-micro scaffolds are developed for long-term healing tissue engineering like cartilage.
30940593	0	37	theme	engineering	180:190	arg1	applications					192:203	cartilage tissue engineering applications	163:203	cartilage tissue engineering applications	163:203	Evaluation of physical, mechanical and biological properties of poly 3-hydroxybutyrate-chitosan-multiwalled carbon nanotube/silk nano-micro composite scaffold for cartilage tissue engineering applications.
30940593	4	38	theme	hydrophilicity	861:874	arg1	properties					876:885	the hydrophilicity properties	857:885	the hydrophilicity properties	857:885	The saturation condition as well as MWNTs leads to intensification in the hydrophilicity properties.
30940593	0	39	theme	mechanical	24:33	arg1	properties					50:59	physical, mechanical and biological properties	14:59	physical, mechanical and biological properties of poly 3-hydroxybutyrate-chitosan-multiwalled carbon nanotube/silk nano-micro composite scaffold for cartilage tissue engineering applications	14:203	Evaluation of physical, mechanical and biological properties of poly 3-hydroxybutyrate-chitosan-multiwalled carbon nanotube/silk nano-micro composite scaffold for cartilage tissue engineering applications.
30940593	0	40	theme	tissue	173:178	arg1	applications					192:203	cartilage tissue engineering applications	163:203	cartilage tissue engineering applications	163:203	Evaluation of physical, mechanical and biological properties of poly 3-hydroxybutyrate-chitosan-multiwalled carbon nanotube/silk nano-micro composite scaffold for cartilage tissue engineering applications.
30940593	3	41	theme	fiber	682:686	arg1	diameter					688:695	fiber diameter	682:695	fiber diameter	682:695	Consuming MWNTs lead to a decrease in fiber diameter, while an increase in specific surface area, tensile strength and bioactivity properties.
30940593	0	42	theme	properties	50:59	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of physical, mechanical and biological properties of poly 3-hydroxybutyrate-chitosan-multiwalled carbon nanotube/silk nano-micro composite scaffold for cartilage tissue engineering applications.	0:204	Evaluation of physical, mechanical and biological properties of poly 3-hydroxybutyrate-chitosan-multiwalled carbon nanotube/silk nano-micro composite scaffold for cartilage tissue engineering applications.
30940593	2	43	dep	unsaturated	553:563	arg1	U					566:566	U	566:566	U	566:566	The poly 3-hydroxybutyrate (P3HB)-chitosan/silk and P3HB-chitosan-1 wt% multi-walled carbon nanotubes functionalized by COOH (MWNTs)/silk nano-micro scaffolds are fabricated through electrospinning the solution on a knitted silk which is saturated (S) or unsaturated (U) with P3HB as a mediator to enhance the interaction at nano/microinterface.
30940593	6	44	theme	containing	1014:1023	arg1	MWNTs					1025:1029	The scaffold containing MWNTs	1001:1029	The scaffold containing MWNTs	1001:1029	The scaffold containing MWNTs showed slower degradation rate.
30940593	2	45	theme	MWNTs	424:428	arg1	scaffolds					447:455	COOH (MWNTs)/silk nano-micro scaffolds	418:455	COOH (MWNTs)/silk nano-micro scaffolds	418:455	The poly 3-hydroxybutyrate (P3HB)-chitosan/silk and P3HB-chitosan-1 wt% multi-walled carbon nanotubes functionalized by COOH (MWNTs)/silk nano-micro scaffolds are fabricated through electrospinning the solution on a knitted silk which is saturated (S) or unsaturated (U) with P3HB as a mediator to enhance the interaction at nano/microinterface.
30940593	0	46	theme	biological	39:48	arg1	properties					50:59	physical, mechanical and biological properties	14:59	physical, mechanical and biological properties of poly 3-hydroxybutyrate-chitosan-multiwalled carbon nanotube/silk nano-micro composite scaffold for cartilage tissue engineering applications	14:203	Evaluation of physical, mechanical and biological properties of poly 3-hydroxybutyrate-chitosan-multiwalled carbon nanotube/silk nano-micro composite scaffold for cartilage tissue engineering applications.
30940593	3	47	theme	Consuming	644:652	arg1	MWNTs					654:658	Consuming MWNTs	644:658	Consuming MWNTs	644:658	Consuming MWNTs lead to a decrease in fiber diameter, while an increase in specific surface area, tensile strength and bioactivity properties.
30940593	8	48	theme	P3HB-chitosan-MWNTs/silk	1180:1203	arg1	appropriate					1235:1245	appropriate	1235:1245	appropriate	1235:1245	The P3HB-chitosan-MWNTs/silk (S) nano-microscaffold can be appropriate for a long-term tissue engineering application like cartilage.
30940593	8	48	theme	P3HB-chitosan-MWNTs/silk	1180:1203	arg1	S					1206:1206	The P3HB-chitosan-MWNTs/silk (S)	1176:1207	The P3HB-chitosan-MWNTs/silk (S) nano-microscaffold	1176:1226	The P3HB-chitosan-MWNTs/silk (S) nano-microscaffold can be appropriate for a long-term tissue engineering application like cartilage.
30940593	3	49	theme	specific	719:726	arg1	area					736:739	specific surface area	719:739	specific surface area	719:739	Consuming MWNTs lead to a decrease in fiber diameter, while an increase in specific surface area, tensile strength and bioactivity properties.
30940593	5	50	from	nanolayer	892:900	arg1	scaffolds					909:917	all scaffolds	905:917	all scaffolds	905:917	The nanolayer in all scaffolds lead to an increase in tensile strength in comparison with the pure knitted silk.
30940593	2	51	theme	COOH	418:421	arg1	scaffolds					447:455	COOH (MWNTs)/silk nano-micro scaffolds	418:455	COOH (MWNTs)/silk nano-micro scaffolds	418:455	The poly 3-hydroxybutyrate (P3HB)-chitosan/silk and P3HB-chitosan-1 wt% multi-walled carbon nanotubes functionalized by COOH (MWNTs)/silk nano-micro scaffolds are fabricated through electrospinning the solution on a knitted silk which is saturated (S) or unsaturated (U) with P3HB as a mediator to enhance the interaction at nano/microinterface.
30940593	6	52	theme	slower	1038:1043	arg1	rate					1057:1060	slower degradation rate	1038:1060	slower degradation rate	1038:1060	The scaffold containing MWNTs showed slower degradation rate.
30940593	2	53	from	solution	500:507	arg1	S					547:547	S	547:547	S	547:547	The poly 3-hydroxybutyrate (P3HB)-chitosan/silk and P3HB-chitosan-1 wt% multi-walled carbon nanotubes functionalized by COOH (MWNTs)/silk nano-micro scaffolds are fabricated through electrospinning the solution on a knitted silk which is saturated (S) or unsaturated (U) with P3HB as a mediator to enhance the interaction at nano/microinterface.
30940593	2	53	from	solution	500:507	arg1	silk					522:525	a knitted silk	512:525	a knitted silk which is saturated (S) or unsaturated (U) with P3HB as a mediator to enhance the interaction at nano/microinterface	512:641	The poly 3-hydroxybutyrate (P3HB)-chitosan/silk and P3HB-chitosan-1 wt% multi-walled carbon nanotubes functionalized by COOH (MWNTs)/silk nano-micro scaffolds are fabricated through electrospinning the solution on a knitted silk which is saturated (S) or unsaturated (U) with P3HB as a mediator to enhance the interaction at nano/microinterface.
30940593	2	53	from	solution	500:507	arg1	saturated					536:544	saturated	536:544	saturated	536:544	The poly 3-hydroxybutyrate (P3HB)-chitosan/silk and P3HB-chitosan-1 wt% multi-walled carbon nanotubes functionalized by COOH (MWNTs)/silk nano-micro scaffolds are fabricated through electrospinning the solution on a knitted silk which is saturated (S) or unsaturated (U) with P3HB as a mediator to enhance the interaction at nano/microinterface.
30940593	0	54	theme	poly	64:67	arg1	scaffold					150:157	poly 3-hydroxybutyrate-chitosan-multiwalled carbon nanotube/silk nano-micro composite scaffold	64:157	poly 3-hydroxybutyrate-chitosan-multiwalled carbon nanotube/silk nano-micro composite scaffold for cartilage tissue engineering applications	64:203	Evaluation of physical, mechanical and biological properties of poly 3-hydroxybutyrate-chitosan-multiwalled carbon nanotube/silk nano-micro composite scaffold for cartilage tissue engineering applications.
30940593	2	55	with	saturated	536:544	arg1	P3HB					574:577	P3HB	574:577	P3HB	574:577	The poly 3-hydroxybutyrate (P3HB)-chitosan/silk and P3HB-chitosan-1 wt% multi-walled carbon nanotubes functionalized by COOH (MWNTs)/silk nano-micro scaffolds are fabricated through electrospinning the solution on a knitted silk which is saturated (S) or unsaturated (U) with P3HB as a mediator to enhance the interaction at nano/microinterface.
30940593	2	56	theme	/silk	430:434	arg1	scaffolds					447:455	COOH (MWNTs)/silk nano-micro scaffolds	418:455	COOH (MWNTs)/silk nano-micro scaffolds	418:455	The poly 3-hydroxybutyrate (P3HB)-chitosan/silk and P3HB-chitosan-1 wt% multi-walled carbon nanotubes functionalized by COOH (MWNTs)/silk nano-micro scaffolds are fabricated through electrospinning the solution on a knitted silk which is saturated (S) or unsaturated (U) with P3HB as a mediator to enhance the interaction at nano/microinterface.
30940593	3	57	from	increase	707:714	arg1	bioactivity					763:773	bioactivity	763:773	bioactivity	763:773	Consuming MWNTs lead to a decrease in fiber diameter, while an increase in specific surface area, tensile strength and bioactivity properties.
30940593	3	57	from	increase	707:714	arg1	area					736:739	specific surface area	719:739	specific surface area	719:739	Consuming MWNTs lead to a decrease in fiber diameter, while an increase in specific surface area, tensile strength and bioactivity properties.
30940593	3	57	from	increase	707:714	arg1	strength					750:757	tensile strength	742:757	tensile strength	742:757	Consuming MWNTs lead to a decrease in fiber diameter, while an increase in specific surface area, tensile strength and bioactivity properties.
30940593	2	58	theme	knitted	514:520	arg1	S					547:547	S	547:547	S	547:547	The poly 3-hydroxybutyrate (P3HB)-chitosan/silk and P3HB-chitosan-1 wt% multi-walled carbon nanotubes functionalized by COOH (MWNTs)/silk nano-micro scaffolds are fabricated through electrospinning the solution on a knitted silk which is saturated (S) or unsaturated (U) with P3HB as a mediator to enhance the interaction at nano/microinterface.
30940593	2	58	theme	knitted	514:520	arg1	silk					522:525	a knitted silk	512:525	a knitted silk which is saturated (S) or unsaturated (U) with P3HB as a mediator to enhance the interaction at nano/microinterface	512:641	The poly 3-hydroxybutyrate (P3HB)-chitosan/silk and P3HB-chitosan-1 wt% multi-walled carbon nanotubes functionalized by COOH (MWNTs)/silk nano-micro scaffolds are fabricated through electrospinning the solution on a knitted silk which is saturated (S) or unsaturated (U) with P3HB as a mediator to enhance the interaction at nano/microinterface.
30940593	2	58	theme	knitted	514:520	arg1	saturated					536:544	saturated	536:544	saturated	536:544	The poly 3-hydroxybutyrate (P3HB)-chitosan/silk and P3HB-chitosan-1 wt% multi-walled carbon nanotubes functionalized by COOH (MWNTs)/silk nano-micro scaffolds are fabricated through electrospinning the solution on a knitted silk which is saturated (S) or unsaturated (U) with P3HB as a mediator to enhance the interaction at nano/microinterface.
30940593	2	59	theme	nano-micro	436:445	arg1	scaffolds					447:455	COOH (MWNTs)/silk nano-micro scaffolds	418:455	COOH (MWNTs)/silk nano-micro scaffolds	418:455	The poly 3-hydroxybutyrate (P3HB)-chitosan/silk and P3HB-chitosan-1 wt% multi-walled carbon nanotubes functionalized by COOH (MWNTs)/silk nano-micro scaffolds are fabricated through electrospinning the solution on a knitted silk which is saturated (S) or unsaturated (U) with P3HB as a mediator to enhance the interaction at nano/microinterface.
30474707	7	0	theme	length	653:658	arg1	polymorphism					660:671	amplified fragment length polymorphism	634:671	amplified fragment length polymorphism	634:671	However, the analysis results of pheS gene sequencing, amplified fragment length polymorphism, phenotypic profiles, cellular fatty acid, cell-wall monosaccharide determination, and cell morphology revealed that the novel strain was obviously different from the type strain L. futsaii JCM17355T, and had genetic relationship with Weissella cibaria to a certain degree, suggesting that the novel strain represents a novel subspecies of L. futsaii, for which the following names are proposed: L. futsaii subsp.
30474707	7	1	contain	had	878:880	arg2	relationship					890:901	genetic relationship	882:901	genetic relationship with Weissella cibaria	882:924	However, the analysis results of pheS gene sequencing, amplified fragment length polymorphism, phenotypic profiles, cellular fatty acid, cell-wall monosaccharide determination, and cell morphology revealed that the novel strain was obviously different from the type strain L. futsaii JCM17355T, and had genetic relationship with Weissella cibaria to a certain degree, suggesting that the novel strain represents a novel subspecies of L. futsaii, for which the following names are proposed: L. futsaii subsp.
30474707	7	1	contain	had	878:880	arg1	results					601:607	the analysis results	588:607	the analysis results of pheS gene sequencing, amplified fragment length polymorphism, phenotypic profiles, cellular fatty acid, cell-wall monosaccharide determination, and cell morphology	588:774	However, the analysis results of pheS gene sequencing, amplified fragment length polymorphism, phenotypic profiles, cellular fatty acid, cell-wall monosaccharide determination, and cell morphology revealed that the novel strain was obviously different from the type strain L. futsaii JCM17355T, and had genetic relationship with Weissella cibaria to a certain degree, suggesting that the novel strain represents a novel subspecies of L. futsaii, for which the following names are proposed: L. futsaii subsp.
30474707	5	2	theme	G + C	358:362	arg1	content					364:370	the DNA G + C content	350:370	the DNA G + C content	350:370	The optimal temperature for growth was 37 °C and the DNA G + C content was 39.1 mol%.
30474707	5	2	theme	G + C	358:362	arg1	%					384:384	39.1 mol%	376:384	39.1 mol%	376:384	The optimal temperature for growth was 37 °C and the DNA G + C content was 39.1 mol%.
30474707	6	3	theme	sequencing	425:434	arg1	results					391:397	The results	387:397	The results of 16S rRNA and rpoA gene sequencing, DNA-DNA hybridization, and peptidoglycan type analyses	387:490	The results of 16S rRNA and rpoA gene sequencing, DNA-DNA hybridization, and peptidoglycan type analyses indicated that strain CQ16Z1T belongs to the recognized species Lactobacillus futsaii.
30474707	9	4	dep	subsp	1171:1175	arg1	L.					1160:1161	L.	1160:1161	L.	1160:1161	nov. (type strain YM0097T = JCM 17355T = BCRC 80278T) and L. futsaii subsp.
30474707	9	4	dep	subsp	1171:1175	arg1	nov					1102:1104	nov	1102:1104	nov	1102:1104	nov. (type strain YM0097T = JCM 17355T = BCRC 80278T) and L. futsaii subsp.
30474707	9	4	dep	subsp	1171:1175	arg1	80278T					1148:1153	type strain YM0097T = JCM 17355T = BCRC 80278T	1108:1153	type strain YM0097T = JCM 17355T = BCRC 80278T	1108:1153	nov. (type strain YM0097T = JCM 17355T = BCRC 80278T) and L. futsaii subsp.
30474707	7	5	theme	determination	741:753	arg1	results					601:607	the analysis results	588:607	the analysis results of pheS gene sequencing, amplified fragment length polymorphism, phenotypic profiles, cellular fatty acid, cell-wall monosaccharide determination, and cell morphology	588:774	However, the analysis results of pheS gene sequencing, amplified fragment length polymorphism, phenotypic profiles, cellular fatty acid, cell-wall monosaccharide determination, and cell morphology revealed that the novel strain was obviously different from the type strain L. futsaii JCM17355T, and had genetic relationship with Weissella cibaria to a certain degree, suggesting that the novel strain represents a novel subspecies of L. futsaii, for which the following names are proposed: L. futsaii subsp.
30474707	5	6	theme	optimal	305:311	arg1	temperature					313:323	The optimal temperature	301:323	The optimal temperature for growth	301:334	The optimal temperature for growth was 37 °C and the DNA G + C content was 39.1 mol%.
30474707	6	7	theme	gene	420:423	arg1	sequencing					425:434	rpoA gene sequencing	415:434	rpoA gene sequencing	415:434	The results of 16S rRNA and rpoA gene sequencing, DNA-DNA hybridization, and peptidoglycan type analyses indicated that strain CQ16Z1T belongs to the recognized species Lactobacillus futsaii.
30474707	7	8	theme	novel	967:971	arg1	strain					973:978	the novel strain	963:978	the novel strain	963:978	However, the analysis results of pheS gene sequencing, amplified fragment length polymorphism, phenotypic profiles, cellular fatty acid, cell-wall monosaccharide determination, and cell morphology revealed that the novel strain was obviously different from the type strain L. futsaii JCM17355T, and had genetic relationship with Weissella cibaria to a certain degree, suggesting that the novel strain represents a novel subspecies of L. futsaii, for which the following names are proposed: L. futsaii subsp.
30474707	7	9	theme	analysis	592:599	arg1	results					601:607	the analysis results	588:607	the analysis results of pheS gene sequencing, amplified fragment length polymorphism, phenotypic profiles, cellular fatty acid, cell-wall monosaccharide determination, and cell morphology	588:774	However, the analysis results of pheS gene sequencing, amplified fragment length polymorphism, phenotypic profiles, cellular fatty acid, cell-wall monosaccharide determination, and cell morphology revealed that the novel strain was obviously different from the type strain L. futsaii JCM17355T, and had genetic relationship with Weissella cibaria to a certain degree, suggesting that the novel strain represents a novel subspecies of L. futsaii, for which the following names are proposed: L. futsaii subsp.
30474707	10	10	theme	strain	1216:1221	arg1	CQ16Z1T					1223:1229	the type strain CQ16Z1T	1207:1229	the type strain CQ16Z1T (= CCTCC AB2017187T = KCTC 21089T)	1207:1264	chongqingii subsp.nov., with the type strain CQ16Z1T (= CCTCC AB2017187T = KCTC 21089T).
30474707	10	10	theme	strain	1216:1221	arg1	21089T					1258:1263	= CCTCC AB2017187T = KCTC 21089T	1232:1263	= CCTCC AB2017187T = KCTC 21089T	1232:1263	chongqingii subsp.nov., with the type strain CQ16Z1T (= CCTCC AB2017187T = KCTC 21089T).
30474707	7	11	theme	gene	617:620	arg1	sequencing					622:631	pheS gene sequencing	612:631	pheS gene sequencing	612:631	However, the analysis results of pheS gene sequencing, amplified fragment length polymorphism, phenotypic profiles, cellular fatty acid, cell-wall monosaccharide determination, and cell morphology revealed that the novel strain was obviously different from the type strain L. futsaii JCM17355T, and had genetic relationship with Weissella cibaria to a certain degree, suggesting that the novel strain represents a novel subspecies of L. futsaii, for which the following names are proposed: L. futsaii subsp.
30474707	6	12	theme	rpoA	415:418	arg1	sequencing					425:434	rpoA gene sequencing	415:434	rpoA gene sequencing	415:434	The results of 16S rRNA and rpoA gene sequencing, DNA-DNA hybridization, and peptidoglycan type analyses indicated that strain CQ16Z1T belongs to the recognized species Lactobacillus futsaii.
30474707	3	13	theme	traditional	146:156	arg1	pickle					166:171	a traditional Chinese pickle	144:171	a traditional Chinese pickle	144:171	Strain CQ16Z1T was isolated from jamiecosley, a traditional Chinese pickle.
30474707	3	13	theme	traditional	146:156	arg1	jamiecosley					131:141	jamiecosley	131:141	jamiecosley	131:141	Strain CQ16Z1T was isolated from jamiecosley, a traditional Chinese pickle.
30474707	7	14	dep	subspecies	999:1008	arg1	L.					1069:1070	L.	1069:1070	L.	1069:1070	However, the analysis results of pheS gene sequencing, amplified fragment length polymorphism, phenotypic profiles, cellular fatty acid, cell-wall monosaccharide determination, and cell morphology revealed that the novel strain was obviously different from the type strain L. futsaii JCM17355T, and had genetic relationship with Weissella cibaria to a certain degree, suggesting that the novel strain represents a novel subspecies of L. futsaii, for which the following names are proposed: L. futsaii subsp.
30474707	5	15	theme	39.1 mol	376:383	arg1	content					364:370	the DNA G + C content	350:370	the DNA G + C content	350:370	The optimal temperature for growth was 37 °C and the DNA G + C content was 39.1 mol%.
30474707	5	15	theme	39.1 mol	376:383	arg1	%					384:384	39.1 mol%	376:384	39.1 mol%	376:384	The optimal temperature for growth was 37 °C and the DNA G + C content was 39.1 mol%.
30474707	6	16	theme	strain	507:512	arg1	CQ16Z1T					514:520	strain CQ16Z1T	507:520	strain CQ16Z1T	507:520	The results of 16S rRNA and rpoA gene sequencing, DNA-DNA hybridization, and peptidoglycan type analyses indicated that strain CQ16Z1T belongs to the recognized species Lactobacillus futsaii.
30474707	7	17	theme	sequencing	622:631	arg1	results					601:607	the analysis results	588:607	the analysis results of pheS gene sequencing, amplified fragment length polymorphism, phenotypic profiles, cellular fatty acid, cell-wall monosaccharide determination, and cell morphology	588:774	However, the analysis results of pheS gene sequencing, amplified fragment length polymorphism, phenotypic profiles, cellular fatty acid, cell-wall monosaccharide determination, and cell morphology revealed that the novel strain was obviously different from the type strain L. futsaii JCM17355T, and had genetic relationship with Weissella cibaria to a certain degree, suggesting that the novel strain represents a novel subspecies of L. futsaii, for which the following names are proposed: L. futsaii subsp.
30474707	3	18	theme	Chinese	158:164	arg1	pickle					166:171	a traditional Chinese pickle	144:171	a traditional Chinese pickle	144:171	Strain CQ16Z1T was isolated from jamiecosley, a traditional Chinese pickle.
30474707	3	18	theme	Chinese	158:164	arg1	jamiecosley					131:141	jamiecosley	131:141	jamiecosley	131:141	Strain CQ16Z1T was isolated from jamiecosley, a traditional Chinese pickle.
30474707	7	19	theme	cell-wall	716:724	arg1	determination					741:753	cell-wall monosaccharide determination	716:753	cell-wall monosaccharide determination	716:753	However, the analysis results of pheS gene sequencing, amplified fragment length polymorphism, phenotypic profiles, cellular fatty acid, cell-wall monosaccharide determination, and cell morphology revealed that the novel strain was obviously different from the type strain L. futsaii JCM17355T, and had genetic relationship with Weissella cibaria to a certain degree, suggesting that the novel strain represents a novel subspecies of L. futsaii, for which the following names are proposed: L. futsaii subsp.
30474707	6	20	theme	rRNA	406:409	arg1	results					391:397	The results	387:397	The results of 16S rRNA and rpoA gene sequencing, DNA-DNA hybridization, and peptidoglycan type analyses	387:490	The results of 16S rRNA and rpoA gene sequencing, DNA-DNA hybridization, and peptidoglycan type analyses indicated that strain CQ16Z1T belongs to the recognized species Lactobacillus futsaii.
30474707	7	21	dep	different	821:829	arg1	L.					852:853	L.	852:853	L.	852:853	However, the analysis results of pheS gene sequencing, amplified fragment length polymorphism, phenotypic profiles, cellular fatty acid, cell-wall monosaccharide determination, and cell morphology revealed that the novel strain was obviously different from the type strain L. futsaii JCM17355T, and had genetic relationship with Weissella cibaria to a certain degree, suggesting that the novel strain represents a novel subspecies of L. futsaii, for which the following names are proposed: L. futsaii subsp.
30474707	0	22	theme	futsaii	14:20	arg1	subsp					22:26	Lactobacillus futsaii subsp	0:26	Lactobacillus futsaii subsp.	0:27	Lactobacillus futsaii subsp.
30474707	7	23	theme	pheS	612:615	arg1	sequencing					622:631	pheS gene sequencing	612:631	pheS gene sequencing	612:631	However, the analysis results of pheS gene sequencing, amplified fragment length polymorphism, phenotypic profiles, cellular fatty acid, cell-wall monosaccharide determination, and cell morphology revealed that the novel strain was obviously different from the type strain L. futsaii JCM17355T, and had genetic relationship with Weissella cibaria to a certain degree, suggesting that the novel strain represents a novel subspecies of L. futsaii, for which the following names are proposed: L. futsaii subsp.
30474707	6	24	theme	16S	402:404	arg1	rRNA					406:409	16S rRNA	402:409	16S rRNA	402:409	The results of 16S rRNA and rpoA gene sequencing, DNA-DNA hybridization, and peptidoglycan type analyses indicated that strain CQ16Z1T belongs to the recognized species Lactobacillus futsaii.
30474707	10	25	theme	AB2017187T = KCTC	1240:1256	arg1	CQ16Z1T					1223:1229	the type strain CQ16Z1T	1207:1229	the type strain CQ16Z1T (= CCTCC AB2017187T = KCTC 21089T)	1207:1264	chongqingii subsp.nov., with the type strain CQ16Z1T (= CCTCC AB2017187T = KCTC 21089T).
30474707	10	25	theme	AB2017187T = KCTC	1240:1256	arg1	21089T					1258:1263	= CCTCC AB2017187T = KCTC 21089T	1232:1263	= CCTCC AB2017187T = KCTC 21089T	1232:1263	chongqingii subsp.nov., with the type strain CQ16Z1T (= CCTCC AB2017187T = KCTC 21089T).
30474707	0	26	theme	Lactobacillus	0:12	arg1	subsp					22:26	Lactobacillus futsaii subsp	0:26	Lactobacillus futsaii subsp.	0:27	Lactobacillus futsaii subsp.
30474707	10	27	theme	= CCTCC	1232:1238	arg1	CQ16Z1T					1223:1229	the type strain CQ16Z1T	1207:1229	the type strain CQ16Z1T (= CCTCC AB2017187T = KCTC 21089T)	1207:1264	chongqingii subsp.nov., with the type strain CQ16Z1T (= CCTCC AB2017187T = KCTC 21089T).
30474707	10	27	theme	= CCTCC	1232:1238	arg1	21089T					1258:1263	= CCTCC AB2017187T = KCTC 21089T	1232:1263	= CCTCC AB2017187T = KCTC 21089T	1232:1263	chongqingii subsp.nov., with the type strain CQ16Z1T (= CCTCC AB2017187T = KCTC 21089T).
30474707	7	28	theme	type	840:843	arg1	strain					845:850	the type strain	836:850	the type strain	836:850	However, the analysis results of pheS gene sequencing, amplified fragment length polymorphism, phenotypic profiles, cellular fatty acid, cell-wall monosaccharide determination, and cell morphology revealed that the novel strain was obviously different from the type strain L. futsaii JCM17355T, and had genetic relationship with Weissella cibaria to a certain degree, suggesting that the novel strain represents a novel subspecies of L. futsaii, for which the following names are proposed: L. futsaii subsp.
30474707	7	29	theme	different	821:829	arg1	JCM17355T					863:871	obviously different from the type strain L. futsaii JCM17355T	811:871	obviously different from the type strain L. futsaii JCM17355T	811:871	However, the analysis results of pheS gene sequencing, amplified fragment length polymorphism, phenotypic profiles, cellular fatty acid, cell-wall monosaccharide determination, and cell morphology revealed that the novel strain was obviously different from the type strain L. futsaii JCM17355T, and had genetic relationship with Weissella cibaria to a certain degree, suggesting that the novel strain represents a novel subspecies of L. futsaii, for which the following names are proposed: L. futsaii subsp.
30474707	7	29	theme	different	821:829	arg1	strain					800:805	the novel strain	790:805	the novel strain	790:805	However, the analysis results of pheS gene sequencing, amplified fragment length polymorphism, phenotypic profiles, cellular fatty acid, cell-wall monosaccharide determination, and cell morphology revealed that the novel strain was obviously different from the type strain L. futsaii JCM17355T, and had genetic relationship with Weissella cibaria to a certain degree, suggesting that the novel strain represents a novel subspecies of L. futsaii, for which the following names are proposed: L. futsaii subsp.
30474707	7	30	theme	certain	931:937	arg1	degree					939:944	a certain degree	929:944	a certain degree	929:944	However, the analysis results of pheS gene sequencing, amplified fragment length polymorphism, phenotypic profiles, cellular fatty acid, cell-wall monosaccharide determination, and cell morphology revealed that the novel strain was obviously different from the type strain L. futsaii JCM17355T, and had genetic relationship with Weissella cibaria to a certain degree, suggesting that the novel strain represents a novel subspecies of L. futsaii, for which the following names are proposed: L. futsaii subsp.
30474707	9	31	theme	YM0097T = JCM	1120:1132	arg1	nov					1102:1104	nov	1102:1104	nov	1102:1104	nov. (type strain YM0097T = JCM 17355T = BCRC 80278T) and L. futsaii subsp.
30474707	9	31	theme	YM0097T = JCM	1120:1132	arg1	80278T					1148:1153	type strain YM0097T = JCM 17355T = BCRC 80278T	1108:1153	type strain YM0097T = JCM 17355T = BCRC 80278T	1108:1153	nov. (type strain YM0097T = JCM 17355T = BCRC 80278T) and L. futsaii subsp.
30474707	7	32	theme	following	1039:1047	arg1	names					1049:1053	the following names	1035:1053	the following names	1035:1053	However, the analysis results of pheS gene sequencing, amplified fragment length polymorphism, phenotypic profiles, cellular fatty acid, cell-wall monosaccharide determination, and cell morphology revealed that the novel strain was obviously different from the type strain L. futsaii JCM17355T, and had genetic relationship with Weissella cibaria to a certain degree, suggesting that the novel strain represents a novel subspecies of L. futsaii, for which the following names are proposed: L. futsaii subsp.
30474707	7	33	theme	monosaccharide	726:739	arg1	determination					741:753	cell-wall monosaccharide determination	716:753	cell-wall monosaccharide determination	716:753	However, the analysis results of pheS gene sequencing, amplified fragment length polymorphism, phenotypic profiles, cellular fatty acid, cell-wall monosaccharide determination, and cell morphology revealed that the novel strain was obviously different from the type strain L. futsaii JCM17355T, and had genetic relationship with Weissella cibaria to a certain degree, suggesting that the novel strain represents a novel subspecies of L. futsaii, for which the following names are proposed: L. futsaii subsp.
30474707	5	34	theme	DNA	354:356	arg1	content					364:370	the DNA G + C content	350:370	the DNA G + C content	350:370	The optimal temperature for growth was 37 °C and the DNA G + C content was 39.1 mol%.
30474707	5	34	theme	DNA	354:356	arg1	%					384:384	39.1 mol%	376:384	39.1 mol%	376:384	The optimal temperature for growth was 37 °C and the DNA G + C content was 39.1 mol%.
30474707	6	35	theme	type	478:481	arg1	analyses					483:490	peptidoglycan type analyses	464:490	peptidoglycan type analyses	464:490	The results of 16S rRNA and rpoA gene sequencing, DNA-DNA hybridization, and peptidoglycan type analyses indicated that strain CQ16Z1T belongs to the recognized species Lactobacillus futsaii.
30474707	7	36	theme	acid	710:713	arg1	results					601:607	the analysis results	588:607	the analysis results of pheS gene sequencing, amplified fragment length polymorphism, phenotypic profiles, cellular fatty acid, cell-wall monosaccharide determination, and cell morphology	588:774	However, the analysis results of pheS gene sequencing, amplified fragment length polymorphism, phenotypic profiles, cellular fatty acid, cell-wall monosaccharide determination, and cell morphology revealed that the novel strain was obviously different from the type strain L. futsaii JCM17355T, and had genetic relationship with Weissella cibaria to a certain degree, suggesting that the novel strain represents a novel subspecies of L. futsaii, for which the following names are proposed: L. futsaii subsp.
30474707	7	37	dep	L.	1069:1070	arg1	futsaii					1072:1078	L. futsaii subsp	1069:1084	a novel subspecies of L. futsaii, for which the following names are proposed: L. futsaii subsp	991:1084	However, the analysis results of pheS gene sequencing, amplified fragment length polymorphism, phenotypic profiles, cellular fatty acid, cell-wall monosaccharide determination, and cell morphology revealed that the novel strain was obviously different from the type strain L. futsaii JCM17355T, and had genetic relationship with Weissella cibaria to a certain degree, suggesting that the novel strain represents a novel subspecies of L. futsaii, for which the following names are proposed: L. futsaii subsp.
30474707	7	37	dep	L.	1069:1070	arg1	subsp					1080:1084	subsp	1080:1084	a novel subspecies of L. futsaii, for which the following names are proposed: L. futsaii subsp	991:1084	However, the analysis results of pheS gene sequencing, amplified fragment length polymorphism, phenotypic profiles, cellular fatty acid, cell-wall monosaccharide determination, and cell morphology revealed that the novel strain was obviously different from the type strain L. futsaii JCM17355T, and had genetic relationship with Weissella cibaria to a certain degree, suggesting that the novel strain represents a novel subspecies of L. futsaii, for which the following names are proposed: L. futsaii subsp.
30474707	6	38	theme	peptidoglycan	464:476	arg1	analyses					483:490	peptidoglycan type analyses	464:490	peptidoglycan type analyses	464:490	The results of 16S rRNA and rpoA gene sequencing, DNA-DNA hybridization, and peptidoglycan type analyses indicated that strain CQ16Z1T belongs to the recognized species Lactobacillus futsaii.
30474707	7	39	dep	L.	852:853	arg1	futsaii					855:861	futsaii	855:861	futsaii	855:861	However, the analysis results of pheS gene sequencing, amplified fragment length polymorphism, phenotypic profiles, cellular fatty acid, cell-wall monosaccharide determination, and cell morphology revealed that the novel strain was obviously different from the type strain L. futsaii JCM17355T, and had genetic relationship with Weissella cibaria to a certain degree, suggesting that the novel strain represents a novel subspecies of L. futsaii, for which the following names are proposed: L. futsaii subsp.
30474707	7	40	theme	novel	794:798	arg1	JCM17355T					863:871	obviously different from the type strain L. futsaii JCM17355T	811:871	obviously different from the type strain L. futsaii JCM17355T	811:871	However, the analysis results of pheS gene sequencing, amplified fragment length polymorphism, phenotypic profiles, cellular fatty acid, cell-wall monosaccharide determination, and cell morphology revealed that the novel strain was obviously different from the type strain L. futsaii JCM17355T, and had genetic relationship with Weissella cibaria to a certain degree, suggesting that the novel strain represents a novel subspecies of L. futsaii, for which the following names are proposed: L. futsaii subsp.
30474707	7	40	theme	novel	794:798	arg1	strain					800:805	the novel strain	790:805	the novel strain	790:805	However, the analysis results of pheS gene sequencing, amplified fragment length polymorphism, phenotypic profiles, cellular fatty acid, cell-wall monosaccharide determination, and cell morphology revealed that the novel strain was obviously different from the type strain L. futsaii JCM17355T, and had genetic relationship with Weissella cibaria to a certain degree, suggesting that the novel strain represents a novel subspecies of L. futsaii, for which the following names are proposed: L. futsaii subsp.
30474707	7	41	theme	cellular	695:702	arg1	acid					710:713	cellular fatty acid	695:713	cellular fatty acid	695:713	However, the analysis results of pheS gene sequencing, amplified fragment length polymorphism, phenotypic profiles, cellular fatty acid, cell-wall monosaccharide determination, and cell morphology revealed that the novel strain was obviously different from the type strain L. futsaii JCM17355T, and had genetic relationship with Weissella cibaria to a certain degree, suggesting that the novel strain represents a novel subspecies of L. futsaii, for which the following names are proposed: L. futsaii subsp.
30474707	7	42	theme	futsaii	1016:1022	arg1	subspecies					999:1008	a novel subspecies	991:1008	a novel subspecies of L. futsaii, for which the following names are proposed: L. futsaii subsp	991:1084	However, the analysis results of pheS gene sequencing, amplified fragment length polymorphism, phenotypic profiles, cellular fatty acid, cell-wall monosaccharide determination, and cell morphology revealed that the novel strain was obviously different from the type strain L. futsaii JCM17355T, and had genetic relationship with Weissella cibaria to a certain degree, suggesting that the novel strain represents a novel subspecies of L. futsaii, for which the following names are proposed: L. futsaii subsp.
30474707	7	43	theme	amplified	634:642	arg1	polymorphism					660:671	amplified fragment length polymorphism	634:671	amplified fragment length polymorphism	634:671	However, the analysis results of pheS gene sequencing, amplified fragment length polymorphism, phenotypic profiles, cellular fatty acid, cell-wall monosaccharide determination, and cell morphology revealed that the novel strain was obviously different from the type strain L. futsaii JCM17355T, and had genetic relationship with Weissella cibaria to a certain degree, suggesting that the novel strain represents a novel subspecies of L. futsaii, for which the following names are proposed: L. futsaii subsp.
30474707	2	44	theme	Chinese	82:88	arg1	Pickle					90:95	a Traditional Chinese Pickle	68:95	a Traditional Chinese Pickle	68:95	nov., Isolated from a Traditional Chinese Pickle.
30474707	7	45	theme	phenotypic	674:683	arg1	profiles					685:692	phenotypic profiles	674:692	phenotypic profiles	674:692	However, the analysis results of pheS gene sequencing, amplified fragment length polymorphism, phenotypic profiles, cellular fatty acid, cell-wall monosaccharide determination, and cell morphology revealed that the novel strain was obviously different from the type strain L. futsaii JCM17355T, and had genetic relationship with Weissella cibaria to a certain degree, suggesting that the novel strain represents a novel subspecies of L. futsaii, for which the following names are proposed: L. futsaii subsp.
30474707	7	46	from	strain	845:850	arg1	different					821:829	different	821:829	different	821:829	However, the analysis results of pheS gene sequencing, amplified fragment length polymorphism, phenotypic profiles, cellular fatty acid, cell-wall monosaccharide determination, and cell morphology revealed that the novel strain was obviously different from the type strain L. futsaii JCM17355T, and had genetic relationship with Weissella cibaria to a certain degree, suggesting that the novel strain represents a novel subspecies of L. futsaii, for which the following names are proposed: L. futsaii subsp.
30474707	3	47	theme	Strain	98:103	arg1	CQ16Z1T					105:111	Strain CQ16Z1T	98:111	Strain CQ16Z1T	98:111	Strain CQ16Z1T was isolated from jamiecosley, a traditional Chinese pickle.
30474707	10	48	theme	type	1211:1214	arg1	CQ16Z1T					1223:1229	the type strain CQ16Z1T	1207:1229	the type strain CQ16Z1T (= CCTCC AB2017187T = KCTC 21089T)	1207:1264	chongqingii subsp.nov., with the type strain CQ16Z1T (= CCTCC AB2017187T = KCTC 21089T).
30474707	10	48	theme	type	1211:1214	arg1	21089T					1258:1263	= CCTCC AB2017187T = KCTC 21089T	1232:1263	= CCTCC AB2017187T = KCTC 21089T	1232:1263	chongqingii subsp.nov., with the type strain CQ16Z1T (= CCTCC AB2017187T = KCTC 21089T).
30474707	2	49	theme	Traditional	70:80	arg1	Pickle					90:95	a Traditional Chinese Pickle	68:95	a Traditional Chinese Pickle	68:95	nov., Isolated from a Traditional Chinese Pickle.
30474707	7	50	theme	fatty	704:708	arg1	acid					710:713	cellular fatty acid	695:713	cellular fatty acid	695:713	However, the analysis results of pheS gene sequencing, amplified fragment length polymorphism, phenotypic profiles, cellular fatty acid, cell-wall monosaccharide determination, and cell morphology revealed that the novel strain was obviously different from the type strain L. futsaii JCM17355T, and had genetic relationship with Weissella cibaria to a certain degree, suggesting that the novel strain represents a novel subspecies of L. futsaii, for which the following names are proposed: L. futsaii subsp.
30474707	7	51	theme	profiles	685:692	arg1	results					601:607	the analysis results	588:607	the analysis results of pheS gene sequencing, amplified fragment length polymorphism, phenotypic profiles, cellular fatty acid, cell-wall monosaccharide determination, and cell morphology	588:774	However, the analysis results of pheS gene sequencing, amplified fragment length polymorphism, phenotypic profiles, cellular fatty acid, cell-wall monosaccharide determination, and cell morphology revealed that the novel strain was obviously different from the type strain L. futsaii JCM17355T, and had genetic relationship with Weissella cibaria to a certain degree, suggesting that the novel strain represents a novel subspecies of L. futsaii, for which the following names are proposed: L. futsaii subsp.
30474707	9	52	theme	type	1108:1111	arg1	nov					1102:1104	nov	1102:1104	nov	1102:1104	nov. (type strain YM0097T = JCM 17355T = BCRC 80278T) and L. futsaii subsp.
30474707	9	52	theme	type	1108:1111	arg1	80278T					1148:1153	type strain YM0097T = JCM 17355T = BCRC 80278T	1108:1153	type strain YM0097T = JCM 17355T = BCRC 80278T	1108:1153	nov. (type strain YM0097T = JCM 17355T = BCRC 80278T) and L. futsaii subsp.
30474707	7	53	theme	cell	760:763	arg1	morphology					765:774	cell morphology	760:774	cell morphology	760:774	However, the analysis results of pheS gene sequencing, amplified fragment length polymorphism, phenotypic profiles, cellular fatty acid, cell-wall monosaccharide determination, and cell morphology revealed that the novel strain was obviously different from the type strain L. futsaii JCM17355T, and had genetic relationship with Weissella cibaria to a certain degree, suggesting that the novel strain represents a novel subspecies of L. futsaii, for which the following names are proposed: L. futsaii subsp.
30474707	6	54	theme	DNA-DNA	437:443	arg1	hybridization					445:457	DNA-DNA hybridization	437:457	DNA-DNA hybridization	437:457	The results of 16S rRNA and rpoA gene sequencing, DNA-DNA hybridization, and peptidoglycan type analyses indicated that strain CQ16Z1T belongs to the recognized species Lactobacillus futsaii.
30474707	7	55	theme	polymorphism	660:671	arg1	results					601:607	the analysis results	588:607	the analysis results of pheS gene sequencing, amplified fragment length polymorphism, phenotypic profiles, cellular fatty acid, cell-wall monosaccharide determination, and cell morphology	588:774	However, the analysis results of pheS gene sequencing, amplified fragment length polymorphism, phenotypic profiles, cellular fatty acid, cell-wall monosaccharide determination, and cell morphology revealed that the novel strain was obviously different from the type strain L. futsaii JCM17355T, and had genetic relationship with Weissella cibaria to a certain degree, suggesting that the novel strain represents a novel subspecies of L. futsaii, for which the following names are proposed: L. futsaii subsp.
30474707	9	56	theme	strain	1113:1118	arg1	nov					1102:1104	nov	1102:1104	nov	1102:1104	nov. (type strain YM0097T = JCM 17355T = BCRC 80278T) and L. futsaii subsp.
30474707	9	56	theme	strain	1113:1118	arg1	80278T					1148:1153	type strain YM0097T = JCM 17355T = BCRC 80278T	1108:1153	type strain YM0097T = JCM 17355T = BCRC 80278T	1108:1153	nov. (type strain YM0097T = JCM 17355T = BCRC 80278T) and L. futsaii subsp.
30474707	9	57	theme	futsaii	1163:1169	arg1	subsp					1171:1175	futsaii subsp	1163:1175	futsaii subsp	1163:1175	nov. (type strain YM0097T = JCM 17355T = BCRC 80278T) and L. futsaii subsp.
30474707	7	58	theme	morphology	765:774	arg1	results					601:607	the analysis results	588:607	the analysis results of pheS gene sequencing, amplified fragment length polymorphism, phenotypic profiles, cellular fatty acid, cell-wall monosaccharide determination, and cell morphology	588:774	However, the analysis results of pheS gene sequencing, amplified fragment length polymorphism, phenotypic profiles, cellular fatty acid, cell-wall monosaccharide determination, and cell morphology revealed that the novel strain was obviously different from the type strain L. futsaii JCM17355T, and had genetic relationship with Weissella cibaria to a certain degree, suggesting that the novel strain represents a novel subspecies of L. futsaii, for which the following names are proposed: L. futsaii subsp.
30474707	7	59	theme	novel	993:997	arg1	subspecies					999:1008	a novel subspecies	991:1008	a novel subspecies of L. futsaii, for which the following names are proposed: L. futsaii subsp	991:1084	However, the analysis results of pheS gene sequencing, amplified fragment length polymorphism, phenotypic profiles, cellular fatty acid, cell-wall monosaccharide determination, and cell morphology revealed that the novel strain was obviously different from the type strain L. futsaii JCM17355T, and had genetic relationship with Weissella cibaria to a certain degree, suggesting that the novel strain represents a novel subspecies of L. futsaii, for which the following names are proposed: L. futsaii subsp.
30474707	3	60	attach	isolated	117:124	arg1	pickle					166:171	a traditional Chinese pickle	144:171	a traditional Chinese pickle	144:171	Strain CQ16Z1T was isolated from jamiecosley, a traditional Chinese pickle.
30474707	3	60	attach	isolated	117:124	arg1	jamiecosley					131:141	jamiecosley	131:141	jamiecosley	131:141	Strain CQ16Z1T was isolated from jamiecosley, a traditional Chinese pickle.
30474707	3	60	attach	isolated	117:124	arg2	CQ16Z1T					105:111	Strain CQ16Z1T	98:111	Strain CQ16Z1T	98:111	Strain CQ16Z1T was isolated from jamiecosley, a traditional Chinese pickle.
30474707	7	61	theme	Weissella	908:916	arg1	cibaria					918:924	Weissella cibaria	908:924	Weissella cibaria	908:924	However, the analysis results of pheS gene sequencing, amplified fragment length polymorphism, phenotypic profiles, cellular fatty acid, cell-wall monosaccharide determination, and cell morphology revealed that the novel strain was obviously different from the type strain L. futsaii JCM17355T, and had genetic relationship with Weissella cibaria to a certain degree, suggesting that the novel strain represents a novel subspecies of L. futsaii, for which the following names are proposed: L. futsaii subsp.
30474707	7	62	with	relationship	890:901	arg1	cibaria					918:924	Weissella cibaria	908:924	Weissella cibaria	908:924	However, the analysis results of pheS gene sequencing, amplified fragment length polymorphism, phenotypic profiles, cellular fatty acid, cell-wall monosaccharide determination, and cell morphology revealed that the novel strain was obviously different from the type strain L. futsaii JCM17355T, and had genetic relationship with Weissella cibaria to a certain degree, suggesting that the novel strain represents a novel subspecies of L. futsaii, for which the following names are proposed: L. futsaii subsp.
30474707	6	63	theme	recognized	537:546	arg1	species					548:554	the recognized species	533:554	the recognized species	533:554	The results of 16S rRNA and rpoA gene sequencing, DNA-DNA hybridization, and peptidoglycan type analyses indicated that strain CQ16Z1T belongs to the recognized species Lactobacillus futsaii.
30474707	7	64	theme	fragment	644:651	arg1	polymorphism					660:671	amplified fragment length polymorphism	634:671	amplified fragment length polymorphism	634:671	However, the analysis results of pheS gene sequencing, amplified fragment length polymorphism, phenotypic profiles, cellular fatty acid, cell-wall monosaccharide determination, and cell morphology revealed that the novel strain was obviously different from the type strain L. futsaii JCM17355T, and had genetic relationship with Weissella cibaria to a certain degree, suggesting that the novel strain represents a novel subspecies of L. futsaii, for which the following names are proposed: L. futsaii subsp.
30474707	9	65	theme	17355T = BCRC	1134:1146	arg1	nov					1102:1104	nov	1102:1104	nov	1102:1104	nov. (type strain YM0097T = JCM 17355T = BCRC 80278T) and L. futsaii subsp.
30474707	9	65	theme	17355T = BCRC	1134:1146	arg1	80278T					1148:1153	type strain YM0097T = JCM 17355T = BCRC 80278T	1108:1153	type strain YM0097T = JCM 17355T = BCRC 80278T	1108:1153	nov. (type strain YM0097T = JCM 17355T = BCRC 80278T) and L. futsaii subsp.
30474707	7	66	theme	genetic	882:888	arg1	relationship					890:901	genetic relationship	882:901	genetic relationship with Weissella cibaria	882:924	However, the analysis results of pheS gene sequencing, amplified fragment length polymorphism, phenotypic profiles, cellular fatty acid, cell-wall monosaccharide determination, and cell morphology revealed that the novel strain was obviously different from the type strain L. futsaii JCM17355T, and had genetic relationship with Weissella cibaria to a certain degree, suggesting that the novel strain represents a novel subspecies of L. futsaii, for which the following names are proposed: L. futsaii subsp.
31003033	13	0	theme	inflammasome	2355:2366	arg1	role					2343:2346	the different role	2329:2346	the different role of the inflammasome in the biological response to large scale and nanoscale biomaterials	2329:2435	Our studies that were performed in large scale chitosan scaffolds, stress out that the process of phagocytosis is pivotal in inflammasome assembly and activation, are rather important since they clearly illustrate the different role of the inflammasome in the biological response to large scale and nanoscale biomaterials.
31003033	7	1	theme	IL-1β	1082:1086	arg1	release					1088:1094	IL-1β release	1082:1094	IL-1β release upon classical NLRP3 inflammasome stimulation	1082:1140	Furthermore, BMDMs and human macrophages cultured in both Ch scaffolds presented a reduction in the number of apoptosis-associated speck-like protein containing a caspase activating recruitment domain (ASC) specks and in IL-1β release upon classical NLRP3 inflammasome stimulation.
31003033	11	2	theme	porous	1764:1769	arg1	scaffolds					1780:1788	3D porous chitosan scaffolds	1761:1788	3D porous chitosan scaffolds	1761:1788	To our knowledge this is the first work that demonstrates the modulatory capacity of 3D porous chitosan scaffolds in the NLRP3 inflammasome activation, because our results show that Ch scaffolds impair NLRP3 inflammasome assembly in macrophages.
31003033	4	3	dep	biomaterials	454:465	arg1	degree					528:533	4% or 15% degree	518:533	4% or 15% degree of acetylation (DA)	518:553	Here we have studied how macroscopic biomaterials, Ch scaffolds with different chemical composition: 4% or 15% degree of acetylation (DA) modulate the activation of the NLRP3 inflammasome in vitro.
31003033	10	4	theme	SIGNIFICANCE	1492:1503	arg1	STATEMENT					1479:1487	STATEMENT	1479:1487	STATEMENT OF SIGNIFICANCE: In this research work we have assessed the role of the NLRP3 inflammasome in the macrophage response to 3D chitosan scaffolds with different degrees of acetylation (DA).	1479:1674	STATEMENT OF SIGNIFICANCE: In this research work we have assessed the role of the NLRP3 inflammasome in the macrophage response to 3D chitosan scaffolds with different degrees of acetylation (DA).
31003033	5	5	theme	bone	663:666	arg1	marrow					668:673	bone marrow	663:673	bone marrow	663:673	For that, we assessed the NLRP3 inflammasome in bone marrow derived mouse macrophages (BMDM) and human macrophages cultured within 3D Ch scaffolds.
31003033	2	6	theme	different	266:274	arg1	pathways					289:296	different inflammatory pathways	266:296	different inflammatory pathways	266:296	However, tissue injury caused by biomaterial implantation lead to the release of danger signals that engage different inflammatory pathways on the host.
31003033	6	7	from	activation	834:843	arg1	macrophages					848:858	macrophages	848:858	macrophages	848:858	We found that both Ch scaffolds did not trigger the NLRP3 inflammasome activation in macrophages.
31003033	13	8	theme	nanoscale	2414:2422	arg1	biomaterials					2424:2435	nanoscale biomaterials	2414:2435	nanoscale biomaterials	2414:2435	Our studies that were performed in large scale chitosan scaffolds, stress out that the process of phagocytosis is pivotal in inflammasome assembly and activation, are rather important since they clearly illustrate the different role of the inflammasome in the biological response to large scale and nanoscale biomaterials.
31003033	6	9	theme	NLRP3	815:819	arg1	activation					834:843	the NLRP3 inflammasome activation	811:843	the NLRP3 inflammasome activation in macrophages	811:858	We found that both Ch scaffolds did not trigger the NLRP3 inflammasome activation in macrophages.
31003033	12	10	theme	powerful	2038:2045	arg1	activator					2047:2055	a powerful activator	2036:2055	a powerful activator of the NLRP3 inflammasome in nanoscale chitosan products	2036:2112	Interestingly, our results are in contrast with studies reported in the literature that indicate that chitosan is a powerful activator of the NLRP3 inflammasome in nanoscale chitosan products.
31003033	12	10	theme	powerful	2038:2045	arg1	chitosan					2024:2031	chitosan	2024:2031	chitosan	2024:2031	Interestingly, our results are in contrast with studies reported in the literature that indicate that chitosan is a powerful activator of the NLRP3 inflammasome in nanoscale chitosan products.
31003033	10	11	theme	research	1514:1521	arg1	work					1523:1526	this research work	1509:1526	this research work	1509:1526	STATEMENT OF SIGNIFICANCE: In this research work we have assessed the role of the NLRP3 inflammasome in the macrophage response to 3D chitosan scaffolds with different degrees of acetylation (DA).
31003033	13	12	theme	inflammasome	2240:2251	arg1	assembly					2253:2260	inflammasome assembly	2240:2260	inflammasome assembly	2240:2260	Our studies that were performed in large scale chitosan scaffolds, stress out that the process of phagocytosis is pivotal in inflammasome assembly and activation, are rather important since they clearly illustrate the different role of the inflammasome in the biological response to large scale and nanoscale biomaterials.
31003033	7	13	theme	recruitment	1043:1053	arg1	ASC					1063:1065	ASC	1063:1065	ASC	1063:1065	Furthermore, BMDMs and human macrophages cultured in both Ch scaffolds presented a reduction in the number of apoptosis-associated speck-like protein containing a caspase activating recruitment domain (ASC) specks and in IL-1β release upon classical NLRP3 inflammasome stimulation.
31003033	7	13	theme	recruitment	1043:1053	arg1	domain					1055:1060	a caspase activating recruitment domain	1022:1060	a caspase activating recruitment domain (ASC) specks	1022:1073	Furthermore, BMDMs and human macrophages cultured in both Ch scaffolds presented a reduction in the number of apoptosis-associated speck-like protein containing a caspase activating recruitment domain (ASC) specks and in IL-1β release upon classical NLRP3 inflammasome stimulation.
31003033	8	14	theme	cell-culture	1351:1362	arg1	plates					1364:1369	cell-culture plates	1351:1369	cell-culture plates	1351:1369	We also found a decrease in proIL-1β in BMDMs after priming with LPS when cultured in Ch scaffolds with DA 4% DA after priming with LPS when compared to Ch scaffolds with 15% DA or to macrophages cultured in cell-culture plates.
31003033	11	15	theme	scaffolds	1780:1788	arg1	capacity					1749:1756	the modulatory capacity	1734:1756	the modulatory capacity of 3D porous chitosan scaffolds	1734:1788	To our knowledge this is the first work that demonstrates the modulatory capacity of 3D porous chitosan scaffolds in the NLRP3 inflammasome activation, because our results show that Ch scaffolds impair NLRP3 inflammasome assembly in macrophages.
31003033	8	16	theme	Ch	1229:1230	arg1	scaffolds					1232:1240	Ch scaffolds	1229:1240	Ch scaffolds with DA 4% DA after priming with LPS when compared to Ch scaffolds with 15% DA or to macrophages cultured in cell-culture plates	1229:1369	We also found a decrease in proIL-1β in BMDMs after priming with LPS when cultured in Ch scaffolds with DA 4% DA after priming with LPS when compared to Ch scaffolds with 15% DA or to macrophages cultured in cell-culture plates.
31003033	7	17	theme	caspase	1024:1030	arg1	ASC					1063:1065	ASC	1063:1065	ASC	1063:1065	Furthermore, BMDMs and human macrophages cultured in both Ch scaffolds presented a reduction in the number of apoptosis-associated speck-like protein containing a caspase activating recruitment domain (ASC) specks and in IL-1β release upon classical NLRP3 inflammasome stimulation.
31003033	7	17	theme	caspase	1024:1030	arg1	domain					1055:1060	a caspase activating recruitment domain	1022:1060	a caspase activating recruitment domain (ASC) specks	1022:1073	Furthermore, BMDMs and human macrophages cultured in both Ch scaffolds presented a reduction in the number of apoptosis-associated speck-like protein containing a caspase activating recruitment domain (ASC) specks and in IL-1β release upon classical NLRP3 inflammasome stimulation.
31003033	11	18	theme	Ch	1858:1859	arg1	scaffolds					1861:1869	Ch scaffolds	1858:1869	Ch scaffolds	1858:1869	To our knowledge this is the first work that demonstrates the modulatory capacity of 3D porous chitosan scaffolds in the NLRP3 inflammasome activation, because our results show that Ch scaffolds impair NLRP3 inflammasome assembly in macrophages.
31003033	9	19	theme	3D	1395:1396	arg1	scaffolds					1401:1409	3D Ch scaffolds	1395:1409	3D Ch scaffolds with different DA	1395:1427	Our results shows that 3D Ch scaffolds with different DA impair NLRP3 inflammasome priming and activation.
31003033	5	20	theme	NLRP3	641:645	arg1	inflammasome					647:658	the NLRP3 inflammasome	637:658	the NLRP3 inflammasome in bone marrow derived mouse macrophages (BMDM) and human macrophages cultured within 3D Ch scaffolds	637:760	For that, we assessed the NLRP3 inflammasome in bone marrow derived mouse macrophages (BMDM) and human macrophages cultured within 3D Ch scaffolds.
31003033	1	21	theme	different	98:106	arg1	applications					119:130	different biomedical applications	98:130	different biomedical applications	98:130	Chitosan (Ch) is used in different biomedical applications to promote tissue repair.
31003033	11	22	theme	NLRP3	1878:1882	arg1	assembly					1897:1904	NLRP3 inflammasome assembly	1878:1904	NLRP3 inflammasome assembly	1878:1904	To our knowledge this is the first work that demonstrates the modulatory capacity of 3D porous chitosan scaffolds in the NLRP3 inflammasome activation, because our results show that Ch scaffolds impair NLRP3 inflammasome assembly in macrophages.
31003033	7	23	theme	speck-like	992:1001	arg1	protein					1003:1009	apoptosis-associated speck-like protein	971:1009	apoptosis-associated speck-like protein containing a caspase activating recruitment domain (ASC) specks	971:1073	Furthermore, BMDMs and human macrophages cultured in both Ch scaffolds presented a reduction in the number of apoptosis-associated speck-like protein containing a caspase activating recruitment domain (ASC) specks and in IL-1β release upon classical NLRP3 inflammasome stimulation.
31003033	2	24	theme	danger	239:244	arg1	signals					246:252	danger signals	239:252	danger signals that engage different inflammatory pathways on the host	239:308	However, tissue injury caused by biomaterial implantation lead to the release of danger signals that engage different inflammatory pathways on the host.
31003033	9	25	theme	different	1416:1424	arg1	DA					1426:1427	different DA	1416:1427	different DA	1416:1427	Our results shows that 3D Ch scaffolds with different DA impair NLRP3 inflammasome priming and activation.
31003033	3	26	theme	implanted	321:329	arg1	materials					331:339	Different implanted materials	311:339	Different implanted materials	311:339	Different implanted materials activate the inflammasome leading to the modulation of the immune response.
31003033	4	27	dep	%	526:526	arg1	15					524:525	15	524:525	15	524:525	Here we have studied how macroscopic biomaterials, Ch scaffolds with different chemical composition: 4% or 15% degree of acetylation (DA) modulate the activation of the NLRP3 inflammasome in vitro.
31003033	4	27	dep	%	526:526	arg1	%					519:519	%	519:519	%	519:519	Here we have studied how macroscopic biomaterials, Ch scaffolds with different chemical composition: 4% or 15% degree of acetylation (DA) modulate the activation of the NLRP3 inflammasome in vitro.
31003033	13	28	theme	scale	2156:2160	arg1	scaffolds					2171:2179	large scale chitosan scaffolds	2150:2179	large scale chitosan scaffolds	2150:2179	Our studies that were performed in large scale chitosan scaffolds, stress out that the process of phagocytosis is pivotal in inflammasome assembly and activation, are rather important since they clearly illustrate the different role of the inflammasome in the biological response to large scale and nanoscale biomaterials.
31003033	4	29	theme	chemical	496:503	arg1	composition					505:515	different chemical composition	486:515	different chemical composition	486:515	Here we have studied how macroscopic biomaterials, Ch scaffolds with different chemical composition: 4% or 15% degree of acetylation (DA) modulate the activation of the NLRP3 inflammasome in vitro.
31003033	13	30	theme	biological	2375:2384	arg1	response					2386:2393	the biological response	2371:2393	the biological response to large scale and nanoscale biomaterials	2371:2435	Our studies that were performed in large scale chitosan scaffolds, stress out that the process of phagocytosis is pivotal in inflammasome assembly and activation, are rather important since they clearly illustrate the different role of the inflammasome in the biological response to large scale and nanoscale biomaterials.
31003033	9	31	theme	inflammasome	1442:1453	arg1	priming					1455:1461	NLRP3 inflammasome priming	1436:1461	NLRP3 inflammasome priming	1436:1461	Our results shows that 3D Ch scaffolds with different DA impair NLRP3 inflammasome priming and activation.
31003033	11	32	theme	inflammasome	1884:1895	arg1	assembly					1897:1904	NLRP3 inflammasome assembly	1878:1904	NLRP3 inflammasome assembly	1878:1904	To our knowledge this is the first work that demonstrates the modulatory capacity of 3D porous chitosan scaffolds in the NLRP3 inflammasome activation, because our results show that Ch scaffolds impair NLRP3 inflammasome assembly in macrophages.
31003033	13	33	from	role	2343:2346	arg1	response					2386:2393	the biological response	2371:2393	the biological response to large scale and nanoscale biomaterials	2371:2435	Our studies that were performed in large scale chitosan scaffolds, stress out that the process of phagocytosis is pivotal in inflammasome assembly and activation, are rather important since they clearly illustrate the different role of the inflammasome in the biological response to large scale and nanoscale biomaterials.
31003033	11	34	dep	first	1705:1709	arg1	work					1711:1714	work	1711:1714	work	1711:1714	To our knowledge this is the first work that demonstrates the modulatory capacity of 3D porous chitosan scaffolds in the NLRP3 inflammasome activation, because our results show that Ch scaffolds impair NLRP3 inflammasome assembly in macrophages.
31003033	4	35	theme	Ch	468:469	arg1	biomaterials					454:465	macroscopic biomaterials	442:465	macroscopic biomaterials	442:465	Here we have studied how macroscopic biomaterials, Ch scaffolds with different chemical composition: 4% or 15% degree of acetylation (DA) modulate the activation of the NLRP3 inflammasome in vitro.
31003033	4	35	theme	Ch	468:469	arg1	scaffolds					471:479	Ch scaffolds	468:479	Ch scaffolds with different chemical composition	468:515	Here we have studied how macroscopic biomaterials, Ch scaffolds with different chemical composition: 4% or 15% degree of acetylation (DA) modulate the activation of the NLRP3 inflammasome in vitro.
31003033	10	36	theme	NLRP3	1561:1565	arg1	inflammasome					1567:1578	the NLRP3 inflammasome	1557:1578	the NLRP3 inflammasome	1557:1578	STATEMENT OF SIGNIFICANCE: In this research work we have assessed the role of the NLRP3 inflammasome in the macrophage response to 3D chitosan scaffolds with different degrees of acetylation (DA).
31003033	0	37	theme	inflammasome	35:46	arg1	response					48:55	NLRP3 inflammasome response	29:55	NLRP3 inflammasome response in macrophages	29:70	3D chitosan scaffolds impair NLRP3 inflammasome response in macrophages.
31003033	7	38	theme	human	884:888	arg1	macrophages					890:900	human macrophages	884:900	human macrophages	884:900	Furthermore, BMDMs and human macrophages cultured in both Ch scaffolds presented a reduction in the number of apoptosis-associated speck-like protein containing a caspase activating recruitment domain (ASC) specks and in IL-1β release upon classical NLRP3 inflammasome stimulation.
31003033	8	39	theme	Ch	1296:1297	arg1	scaffolds					1299:1307	Ch scaffolds	1296:1307	Ch scaffolds with 15% DA	1296:1319	We also found a decrease in proIL-1β in BMDMs after priming with LPS when cultured in Ch scaffolds with DA 4% DA after priming with LPS when compared to Ch scaffolds with 15% DA or to macrophages cultured in cell-culture plates.
31003033	0	40	theme	3D	0:1	arg1	scaffolds					12:20	3D chitosan scaffolds	0:20	3D chitosan scaffolds	0:20	3D chitosan scaffolds impair NLRP3 inflammasome response in macrophages.
31003033	10	41	theme	macrophage	1587:1596	arg1	response					1598:1605	the macrophage response	1583:1605	the macrophage response to 3D chitosan scaffolds with different degrees of acetylation (DA)	1583:1673	STATEMENT OF SIGNIFICANCE: In this research work we have assessed the role of the NLRP3 inflammasome in the macrophage response to 3D chitosan scaffolds with different degrees of acetylation (DA).
31003033	3	42	theme	response	407:414	arg1	modulation					382:391	the modulation	378:391	the modulation of the immune response	378:414	Different implanted materials activate the inflammasome leading to the modulation of the immune response.
31003033	4	43	theme	acetylation	538:548	arg1	degree					528:533	4% or 15% degree	518:533	4% or 15% degree of acetylation (DA)	518:553	Here we have studied how macroscopic biomaterials, Ch scaffolds with different chemical composition: 4% or 15% degree of acetylation (DA) modulate the activation of the NLRP3 inflammasome in vitro.
31003033	8	44	theme	%	1316:1316	arg1	DA					1318:1319	15% DA	1314:1319	15% DA	1314:1319	We also found a decrease in proIL-1β in BMDMs after priming with LPS when cultured in Ch scaffolds with DA 4% DA after priming with LPS when compared to Ch scaffolds with 15% DA or to macrophages cultured in cell-culture plates.
31003033	7	45	theme	NLRP3	1111:1115	arg1	stimulation					1130:1140	classical NLRP3 inflammasome stimulation	1101:1140	classical NLRP3 inflammasome stimulation	1101:1140	Furthermore, BMDMs and human macrophages cultured in both Ch scaffolds presented a reduction in the number of apoptosis-associated speck-like protein containing a caspase activating recruitment domain (ASC) specks and in IL-1β release upon classical NLRP3 inflammasome stimulation.
31003033	0	46	theme	NLRP3	29:33	arg1	response					48:55	NLRP3 inflammasome response	29:55	NLRP3 inflammasome response in macrophages	29:70	3D chitosan scaffolds impair NLRP3 inflammasome response in macrophages.
31003033	8	47	with	scaffolds	1232:1240	arg1	DA					1253:1254	DA 4% DA	1247:1254	DA 4% DA after priming with LPS when compared to Ch scaffolds with 15% DA or to macrophages cultured in cell-culture plates	1247:1369	We also found a decrease in proIL-1β in BMDMs after priming with LPS when cultured in Ch scaffolds with DA 4% DA after priming with LPS when compared to Ch scaffolds with 15% DA or to macrophages cultured in cell-culture plates.
31003033	12	48	theme	chitosan	2096:2103	arg1	products					2105:2112	nanoscale chitosan products	2086:2112	nanoscale chitosan products	2086:2112	Interestingly, our results are in contrast with studies reported in the literature that indicate that chitosan is a powerful activator of the NLRP3 inflammasome in nanoscale chitosan products.
31003033	10	49	theme	chitosan	1613:1620	arg1	scaffolds					1622:1630	3D chitosan scaffolds	1610:1630	3D chitosan scaffolds with different degrees of acetylation (DA)	1610:1673	STATEMENT OF SIGNIFICANCE: In this research work we have assessed the role of the NLRP3 inflammasome in the macrophage response to 3D chitosan scaffolds with different degrees of acetylation (DA).
31003033	5	50	theme	Ch	749:750	arg1	scaffolds					752:760	3D Ch scaffolds	746:760	3D Ch scaffolds	746:760	For that, we assessed the NLRP3 inflammasome in bone marrow derived mouse macrophages (BMDM) and human macrophages cultured within 3D Ch scaffolds.
31003033	11	51	theme	3D	1761:1762	arg1	scaffolds					1780:1788	3D porous chitosan scaffolds	1761:1788	3D porous chitosan scaffolds	1761:1788	To our knowledge this is the first work that demonstrates the modulatory capacity of 3D porous chitosan scaffolds in the NLRP3 inflammasome activation, because our results show that Ch scaffolds impair NLRP3 inflammasome assembly in macrophages.
31003033	12	52	theme	inflammasome	2070:2081	arg1	activator					2047:2055	a powerful activator	2036:2055	a powerful activator of the NLRP3 inflammasome in nanoscale chitosan products	2036:2112	Interestingly, our results are in contrast with studies reported in the literature that indicate that chitosan is a powerful activator of the NLRP3 inflammasome in nanoscale chitosan products.
31003033	12	52	theme	inflammasome	2070:2081	arg1	chitosan					2024:2031	chitosan	2024:2031	chitosan	2024:2031	Interestingly, our results are in contrast with studies reported in the literature that indicate that chitosan is a powerful activator of the NLRP3 inflammasome in nanoscale chitosan products.
31003033	12	53	theme	NLRP3	2064:2068	arg1	inflammasome					2070:2081	the NLRP3 inflammasome	2060:2081	the NLRP3 inflammasome in nanoscale chitosan products	2060:2112	Interestingly, our results are in contrast with studies reported in the literature that indicate that chitosan is a powerful activator of the NLRP3 inflammasome in nanoscale chitosan products.
31003033	11	54	theme	chitosan	1771:1778	arg1	scaffolds					1780:1788	3D porous chitosan scaffolds	1761:1788	3D porous chitosan scaffolds	1761:1788	To our knowledge this is the first work that demonstrates the modulatory capacity of 3D porous chitosan scaffolds in the NLRP3 inflammasome activation, because our results show that Ch scaffolds impair NLRP3 inflammasome assembly in macrophages.
31003033	12	55	from	inflammasome	2070:2081	arg1	products					2105:2112	nanoscale chitosan products	2086:2112	nanoscale chitosan products	2086:2112	Interestingly, our results are in contrast with studies reported in the literature that indicate that chitosan is a powerful activator of the NLRP3 inflammasome in nanoscale chitosan products.
31003033	8	56	from	decrease	1159:1166	arg1	proIL-1β					1171:1178	proIL-1β	1171:1178	proIL-1β	1171:1178	We also found a decrease in proIL-1β in BMDMs after priming with LPS when cultured in Ch scaffolds with DA 4% DA after priming with LPS when compared to Ch scaffolds with 15% DA or to macrophages cultured in cell-culture plates.
31003033	13	57	from	pivotal	2229:2235	arg1	activation					2266:2275	activation	2266:2275	activation	2266:2275	Our studies that were performed in large scale chitosan scaffolds, stress out that the process of phagocytosis is pivotal in inflammasome assembly and activation, are rather important since they clearly illustrate the different role of the inflammasome in the biological response to large scale and nanoscale biomaterials.
31003033	13	57	from	pivotal	2229:2235	arg1	assembly					2253:2260	inflammasome assembly	2240:2260	inflammasome assembly	2240:2260	Our studies that were performed in large scale chitosan scaffolds, stress out that the process of phagocytosis is pivotal in inflammasome assembly and activation, are rather important since they clearly illustrate the different role of the inflammasome in the biological response to large scale and nanoscale biomaterials.
31003033	4	58	with	scaffolds	471:479	arg1	composition					505:515	different chemical composition	486:515	different chemical composition	486:515	Here we have studied how macroscopic biomaterials, Ch scaffolds with different chemical composition: 4% or 15% degree of acetylation (DA) modulate the activation of the NLRP3 inflammasome in vitro.
31003033	10	59	dep	STATEMENT	1479:1487	arg1	assessed					1536:1543	assessed	1536:1543	have assessed the role of the NLRP3 inflammasome in the macrophage response to 3D chitosan scaffolds with different degrees of acetylation (DA)	1531:1673	STATEMENT OF SIGNIFICANCE: In this research work we have assessed the role of the NLRP3 inflammasome in the macrophage response to 3D chitosan scaffolds with different degrees of acetylation (DA).
31003033	2	60	theme	inflammatory	276:287	arg1	pathways					289:296	different inflammatory pathways	266:296	different inflammatory pathways	266:296	However, tissue injury caused by biomaterial implantation lead to the release of danger signals that engage different inflammatory pathways on the host.
31003033	12	61	from	activator	2047:2055	arg1	products					2105:2112	nanoscale chitosan products	2086:2112	nanoscale chitosan products	2086:2112	Interestingly, our results are in contrast with studies reported in the literature that indicate that chitosan is a powerful activator of the NLRP3 inflammasome in nanoscale chitosan products.
31003033	9	62	theme	NLRP3	1436:1440	arg1	priming					1455:1461	NLRP3 inflammasome priming	1436:1461	NLRP3 inflammasome priming	1436:1461	Our results shows that 3D Ch scaffolds with different DA impair NLRP3 inflammasome priming and activation.
31003033	10	63	theme	acetylation	1658:1668	arg1	degrees					1647:1653	different degrees	1637:1653	different degrees of acetylation (DA)	1637:1673	STATEMENT OF SIGNIFICANCE: In this research work we have assessed the role of the NLRP3 inflammasome in the macrophage response to 3D chitosan scaffolds with different degrees of acetylation (DA).
31003033	3	64	theme	immune	400:405	arg1	response					407:414	the immune response	396:414	the immune response	396:414	Different implanted materials activate the inflammasome leading to the modulation of the immune response.
31003033	6	65	theme	inflammasome	821:832	arg1	activation					834:843	the NLRP3 inflammasome activation	811:843	the NLRP3 inflammasome activation in macrophages	811:858	We found that both Ch scaffolds did not trigger the NLRP3 inflammasome activation in macrophages.
31003033	12	66	with	contrast	1956:1963	arg1	studies					1970:1976	studies	1970:1976	studies reported in the literature that indicate that chitosan is a powerful activator of the NLRP3 inflammasome in nanoscale chitosan products	1970:2112	Interestingly, our results are in contrast with studies reported in the literature that indicate that chitosan is a powerful activator of the NLRP3 inflammasome in nanoscale chitosan products.
31003033	7	67	from	reduction	944:952	arg1	release					1088:1094	IL-1β release	1082:1094	IL-1β release upon classical NLRP3 inflammasome stimulation	1082:1140	Furthermore, BMDMs and human macrophages cultured in both Ch scaffolds presented a reduction in the number of apoptosis-associated speck-like protein containing a caspase activating recruitment domain (ASC) specks and in IL-1β release upon classical NLRP3 inflammasome stimulation.
31003033	7	67	from	reduction	944:952	arg1	number					961:966	the number	957:966	the number of apoptosis-associated speck-like protein containing a caspase activating recruitment domain (ASC) specks	957:1073	Furthermore, BMDMs and human macrophages cultured in both Ch scaffolds presented a reduction in the number of apoptosis-associated speck-like protein containing a caspase activating recruitment domain (ASC) specks and in IL-1β release upon classical NLRP3 inflammasome stimulation.
31003033	7	68	theme	activating	1032:1041	arg1	ASC					1063:1065	ASC	1063:1065	ASC	1063:1065	Furthermore, BMDMs and human macrophages cultured in both Ch scaffolds presented a reduction in the number of apoptosis-associated speck-like protein containing a caspase activating recruitment domain (ASC) specks and in IL-1β release upon classical NLRP3 inflammasome stimulation.
31003033	7	68	theme	activating	1032:1041	arg1	domain					1055:1060	a caspase activating recruitment domain	1022:1060	a caspase activating recruitment domain (ASC) specks	1022:1073	Furthermore, BMDMs and human macrophages cultured in both Ch scaffolds presented a reduction in the number of apoptosis-associated speck-like protein containing a caspase activating recruitment domain (ASC) specks and in IL-1β release upon classical NLRP3 inflammasome stimulation.
31003033	4	69	theme	macroscopic	442:452	arg1	biomaterials					454:465	macroscopic biomaterials	442:465	macroscopic biomaterials	442:465	Here we have studied how macroscopic biomaterials, Ch scaffolds with different chemical composition: 4% or 15% degree of acetylation (DA) modulate the activation of the NLRP3 inflammasome in vitro.
31003033	4	69	theme	macroscopic	442:452	arg1	scaffolds					471:479	Ch scaffolds	468:479	Ch scaffolds with different chemical composition	468:515	Here we have studied how macroscopic biomaterials, Ch scaffolds with different chemical composition: 4% or 15% degree of acetylation (DA) modulate the activation of the NLRP3 inflammasome in vitro.
31003033	4	70	theme	inflammasome	592:603	arg1	activation					568:577	the activation	564:577	the activation of the NLRP3 inflammasome	564:603	Here we have studied how macroscopic biomaterials, Ch scaffolds with different chemical composition: 4% or 15% degree of acetylation (DA) modulate the activation of the NLRP3 inflammasome in vitro.
31003033	7	71	dep	domain	1055:1060	arg1	specks					1068:1073	specks	1068:1073	a caspase activating recruitment domain (ASC) specks	1022:1073	Furthermore, BMDMs and human macrophages cultured in both Ch scaffolds presented a reduction in the number of apoptosis-associated speck-like protein containing a caspase activating recruitment domain (ASC) specks and in IL-1β release upon classical NLRP3 inflammasome stimulation.
31003033	13	72	theme	large	2398:2402	arg1	scale					2404:2408	large scale	2398:2408	large scale	2398:2408	Our studies that were performed in large scale chitosan scaffolds, stress out that the process of phagocytosis is pivotal in inflammasome assembly and activation, are rather important since they clearly illustrate the different role of the inflammasome in the biological response to large scale and nanoscale biomaterials.
31003033	12	73	from	products	2105:2112	arg1	activator					2047:2055	a powerful activator	2036:2055	a powerful activator of the NLRP3 inflammasome in nanoscale chitosan products	2036:2112	Interestingly, our results are in contrast with studies reported in the literature that indicate that chitosan is a powerful activator of the NLRP3 inflammasome in nanoscale chitosan products.
31003033	12	73	from	products	2105:2112	arg1	chitosan					2024:2031	chitosan	2024:2031	chitosan	2024:2031	Interestingly, our results are in contrast with studies reported in the literature that indicate that chitosan is a powerful activator of the NLRP3 inflammasome in nanoscale chitosan products.
31003033	7	74	theme	protein	1003:1009	arg1	number					961:966	the number	957:966	the number of apoptosis-associated speck-like protein containing a caspase activating recruitment domain (ASC) specks	957:1073	Furthermore, BMDMs and human macrophages cultured in both Ch scaffolds presented a reduction in the number of apoptosis-associated speck-like protein containing a caspase activating recruitment domain (ASC) specks and in IL-1β release upon classical NLRP3 inflammasome stimulation.
31003033	9	75	theme	Ch	1398:1399	arg1	scaffolds					1401:1409	3D Ch scaffolds	1395:1409	3D Ch scaffolds with different DA	1395:1427	Our results shows that 3D Ch scaffolds with different DA impair NLRP3 inflammasome priming and activation.
31003033	6	76	theme	Ch	782:783	arg1	scaffolds					785:793	both Ch scaffolds	777:793	both Ch scaffolds	777:793	We found that both Ch scaffolds did not trigger the NLRP3 inflammasome activation in macrophages.
31003033	7	77	theme	apoptosis-associated	971:990	arg1	protein					1003:1009	apoptosis-associated speck-like protein	971:1009	apoptosis-associated speck-like protein containing a caspase activating recruitment domain (ASC) specks	971:1073	Furthermore, BMDMs and human macrophages cultured in both Ch scaffolds presented a reduction in the number of apoptosis-associated speck-like protein containing a caspase activating recruitment domain (ASC) specks and in IL-1β release upon classical NLRP3 inflammasome stimulation.
31003033	2	78	theme	signals	246:252	arg1	release					228:234	the release	224:234	the release of danger signals that engage different inflammatory pathways on the host	224:308	However, tissue injury caused by biomaterial implantation lead to the release of danger signals that engage different inflammatory pathways on the host.
31003033	1	79	theme	biomedical	108:117	arg1	applications					119:130	different biomedical applications	98:130	different biomedical applications	98:130	Chitosan (Ch) is used in different biomedical applications to promote tissue repair.
31003033	8	80	theme	DA	1247:1248	arg1	DA					1253:1254	DA 4% DA	1247:1254	DA 4% DA after priming with LPS when compared to Ch scaffolds with 15% DA or to macrophages cultured in cell-culture plates	1247:1369	We also found a decrease in proIL-1β in BMDMs after priming with LPS when cultured in Ch scaffolds with DA 4% DA after priming with LPS when compared to Ch scaffolds with 15% DA or to macrophages cultured in cell-culture plates.
31003033	13	81	theme	large	2150:2154	arg1	scaffolds					2171:2179	large scale chitosan scaffolds	2150:2179	large scale chitosan scaffolds	2150:2179	Our studies that were performed in large scale chitosan scaffolds, stress out that the process of phagocytosis is pivotal in inflammasome assembly and activation, are rather important since they clearly illustrate the different role of the inflammasome in the biological response to large scale and nanoscale biomaterials.
31003033	5	82	dep	derived	675:681	arg1	marrow					668:673	bone marrow	663:673	bone marrow	663:673	For that, we assessed the NLRP3 inflammasome in bone marrow derived mouse macrophages (BMDM) and human macrophages cultured within 3D Ch scaffolds.
31003033	8	83	theme	%	1251:1251	arg1	DA					1253:1254	DA 4% DA	1247:1254	DA 4% DA after priming with LPS when compared to Ch scaffolds with 15% DA or to macrophages cultured in cell-culture plates	1247:1369	We also found a decrease in proIL-1β in BMDMs after priming with LPS when cultured in Ch scaffolds with DA 4% DA after priming with LPS when compared to Ch scaffolds with 15% DA or to macrophages cultured in cell-culture plates.
31003033	5	84	theme	human	712:716	arg1	macrophages					718:728	human macrophages	712:728	human macrophages	712:728	For that, we assessed the NLRP3 inflammasome in bone marrow derived mouse macrophages (BMDM) and human macrophages cultured within 3D Ch scaffolds.
31003033	13	85	from	assembly	2253:2260	arg1	pivotal					2229:2235	pivotal	2229:2235	pivotal	2229:2235	Our studies that were performed in large scale chitosan scaffolds, stress out that the process of phagocytosis is pivotal in inflammasome assembly and activation, are rather important since they clearly illustrate the different role of the inflammasome in the biological response to large scale and nanoscale biomaterials.
31003033	13	85	from	assembly	2253:2260	arg1	process					2202:2208	the process	2198:2208	the process of phagocytosis	2198:2224	Our studies that were performed in large scale chitosan scaffolds, stress out that the process of phagocytosis is pivotal in inflammasome assembly and activation, are rather important since they clearly illustrate the different role of the inflammasome in the biological response to large scale and nanoscale biomaterials.
31003033	1	86	theme	tissue	143:148	arg1	repair					150:155	tissue repair	143:155	tissue repair	143:155	Chitosan (Ch) is used in different biomedical applications to promote tissue repair.
31003033	3	87	theme	Different	311:319	arg1	materials					331:339	Different implanted materials	311:339	Different implanted materials	311:339	Different implanted materials activate the inflammasome leading to the modulation of the immune response.
31003033	8	88	with	scaffolds	1299:1307	arg1	DA					1318:1319	15% DA	1314:1319	15% DA	1314:1319	We also found a decrease in proIL-1β in BMDMs after priming with LPS when cultured in Ch scaffolds with DA 4% DA after priming with LPS when compared to Ch scaffolds with 15% DA or to macrophages cultured in cell-culture plates.
31003033	5	89	theme	mouse	683:687	arg1	BMDM					702:705	BMDM	702:705	BMDM	702:705	For that, we assessed the NLRP3 inflammasome in bone marrow derived mouse macrophages (BMDM) and human macrophages cultured within 3D Ch scaffolds.
31003033	5	89	theme	mouse	683:687	arg1	macrophages					689:699	bone marrow derived mouse macrophages	663:699	bone marrow derived mouse macrophages (BMDM)	663:706	For that, we assessed the NLRP3 inflammasome in bone marrow derived mouse macrophages (BMDM) and human macrophages cultured within 3D Ch scaffolds.
31003033	7	90	theme	Ch	919:920	arg1	scaffolds					922:930	both Ch scaffolds	914:930	both Ch scaffolds	914:930	Furthermore, BMDMs and human macrophages cultured in both Ch scaffolds presented a reduction in the number of apoptosis-associated speck-like protein containing a caspase activating recruitment domain (ASC) specks and in IL-1β release upon classical NLRP3 inflammasome stimulation.
31003033	13	91	theme	different	2333:2341	arg1	role					2343:2346	the different role	2329:2346	the different role of the inflammasome in the biological response to large scale and nanoscale biomaterials	2329:2435	Our studies that were performed in large scale chitosan scaffolds, stress out that the process of phagocytosis is pivotal in inflammasome assembly and activation, are rather important since they clearly illustrate the different role of the inflammasome in the biological response to large scale and nanoscale biomaterials.
31003033	13	92	theme	chitosan	2162:2169	arg1	scaffolds					2171:2179	large scale chitosan scaffolds	2150:2179	large scale chitosan scaffolds	2150:2179	Our studies that were performed in large scale chitosan scaffolds, stress out that the process of phagocytosis is pivotal in inflammasome assembly and activation, are rather important since they clearly illustrate the different role of the inflammasome in the biological response to large scale and nanoscale biomaterials.
31003033	11	93	theme	modulatory	1738:1747	arg1	capacity					1749:1756	the modulatory capacity	1734:1756	the modulatory capacity of 3D porous chitosan scaffolds	1734:1788	To our knowledge this is the first work that demonstrates the modulatory capacity of 3D porous chitosan scaffolds in the NLRP3 inflammasome activation, because our results show that Ch scaffolds impair NLRP3 inflammasome assembly in macrophages.
31003033	13	94	from	activation	2266:2275	arg1	pivotal					2229:2235	pivotal	2229:2235	pivotal	2229:2235	Our studies that were performed in large scale chitosan scaffolds, stress out that the process of phagocytosis is pivotal in inflammasome assembly and activation, are rather important since they clearly illustrate the different role of the inflammasome in the biological response to large scale and nanoscale biomaterials.
31003033	13	94	from	activation	2266:2275	arg1	process					2202:2208	the process	2198:2208	the process of phagocytosis	2198:2224	Our studies that were performed in large scale chitosan scaffolds, stress out that the process of phagocytosis is pivotal in inflammasome assembly and activation, are rather important since they clearly illustrate the different role of the inflammasome in the biological response to large scale and nanoscale biomaterials.
31003033	4	95	theme	different	486:494	arg1	composition					505:515	different chemical composition	486:515	different chemical composition	486:515	Here we have studied how macroscopic biomaterials, Ch scaffolds with different chemical composition: 4% or 15% degree of acetylation (DA) modulate the activation of the NLRP3 inflammasome in vitro.
31003033	7	96	contain	containing	1011:1020	arg1	protein					1003:1009	apoptosis-associated speck-like protein	971:1009	apoptosis-associated speck-like protein containing a caspase activating recruitment domain (ASC) specks	971:1073	Furthermore, BMDMs and human macrophages cultured in both Ch scaffolds presented a reduction in the number of apoptosis-associated speck-like protein containing a caspase activating recruitment domain (ASC) specks and in IL-1β release upon classical NLRP3 inflammasome stimulation.
31003033	7	96	contain	containing	1011:1020	arg2	domain					1055:1060	a caspase activating recruitment domain	1022:1060	a caspase activating recruitment domain (ASC) specks	1022:1073	Furthermore, BMDMs and human macrophages cultured in both Ch scaffolds presented a reduction in the number of apoptosis-associated speck-like protein containing a caspase activating recruitment domain (ASC) specks and in IL-1β release upon classical NLRP3 inflammasome stimulation.
31003033	7	96	contain	containing	1011:1020	arg2	ASC					1063:1065	ASC	1063:1065	ASC	1063:1065	Furthermore, BMDMs and human macrophages cultured in both Ch scaffolds presented a reduction in the number of apoptosis-associated speck-like protein containing a caspase activating recruitment domain (ASC) specks and in IL-1β release upon classical NLRP3 inflammasome stimulation.
31003033	11	97	theme	inflammasome	1803:1814	arg1	activation					1816:1825	the NLRP3 inflammasome activation	1793:1825	the NLRP3 inflammasome activation	1793:1825	To our knowledge this is the first work that demonstrates the modulatory capacity of 3D porous chitosan scaffolds in the NLRP3 inflammasome activation, because our results show that Ch scaffolds impair NLRP3 inflammasome assembly in macrophages.
31003033	0	98	theme	chitosan	3:10	arg1	scaffolds					12:20	3D chitosan scaffolds	0:20	3D chitosan scaffolds	0:20	3D chitosan scaffolds impair NLRP3 inflammasome response in macrophages.
31003033	4	99	dep	degree	528:533	arg1	%					526:526	%	526:526	%	526:526	Here we have studied how macroscopic biomaterials, Ch scaffolds with different chemical composition: 4% or 15% degree of acetylation (DA) modulate the activation of the NLRP3 inflammasome in vitro.
31003033	0	100	from	response	48:55	arg1	macrophages					60:70	macrophages	60:70	macrophages	60:70	3D chitosan scaffolds impair NLRP3 inflammasome response in macrophages.
31003033	9	101	with	scaffolds	1401:1409	arg1	DA					1426:1427	different DA	1416:1427	different DA	1416:1427	Our results shows that 3D Ch scaffolds with different DA impair NLRP3 inflammasome priming and activation.
31003033	7	102	theme	inflammasome	1117:1128	arg1	stimulation					1130:1140	classical NLRP3 inflammasome stimulation	1101:1140	classical NLRP3 inflammasome stimulation	1101:1140	Furthermore, BMDMs and human macrophages cultured in both Ch scaffolds presented a reduction in the number of apoptosis-associated speck-like protein containing a caspase activating recruitment domain (ASC) specks and in IL-1β release upon classical NLRP3 inflammasome stimulation.
31003033	2	103	theme	tissue	167:172	arg1	injury					174:179	tissue injury	167:179	tissue injury caused by biomaterial implantation	167:214	However, tissue injury caused by biomaterial implantation lead to the release of danger signals that engage different inflammatory pathways on the host.
31003033	7	104	theme	classical	1101:1109	arg1	stimulation					1130:1140	classical NLRP3 inflammasome stimulation	1101:1140	classical NLRP3 inflammasome stimulation	1101:1140	Furthermore, BMDMs and human macrophages cultured in both Ch scaffolds presented a reduction in the number of apoptosis-associated speck-like protein containing a caspase activating recruitment domain (ASC) specks and in IL-1β release upon classical NLRP3 inflammasome stimulation.
31003033	11	105	theme	NLRP3	1797:1801	arg1	activation					1816:1825	the NLRP3 inflammasome activation	1793:1825	the NLRP3 inflammasome activation	1793:1825	To our knowledge this is the first work that demonstrates the modulatory capacity of 3D porous chitosan scaffolds in the NLRP3 inflammasome activation, because our results show that Ch scaffolds impair NLRP3 inflammasome assembly in macrophages.
31003033	8	106	theme	15	1314:1315	arg1	%					1316:1316	%	1316:1316	%	1316:1316	We also found a decrease in proIL-1β in BMDMs after priming with LPS when cultured in Ch scaffolds with DA 4% DA after priming with LPS when compared to Ch scaffolds with 15% DA or to macrophages cultured in cell-culture plates.
31003033	10	107	theme	inflammasome	1567:1578	arg1	role					1549:1552	the role	1545:1552	the role of the NLRP3 inflammasome in the macrophage response to 3D chitosan scaffolds with different degrees of acetylation (DA)	1545:1673	STATEMENT OF SIGNIFICANCE: In this research work we have assessed the role of the NLRP3 inflammasome in the macrophage response to 3D chitosan scaffolds with different degrees of acetylation (DA).
31003033	5	108	theme	3D	746:747	arg1	scaffolds					752:760	3D Ch scaffolds	746:760	3D Ch scaffolds	746:760	For that, we assessed the NLRP3 inflammasome in bone marrow derived mouse macrophages (BMDM) and human macrophages cultured within 3D Ch scaffolds.
31003033	10	109	from	role	1549:1552	arg1	response					1598:1605	the macrophage response	1583:1605	the macrophage response to 3D chitosan scaffolds with different degrees of acetylation (DA)	1583:1673	STATEMENT OF SIGNIFICANCE: In this research work we have assessed the role of the NLRP3 inflammasome in the macrophage response to 3D chitosan scaffolds with different degrees of acetylation (DA).
31003033	1	110	used	used	90:93	arg2	Ch					83:84	Ch	83:84	Ch	83:84	Chitosan (Ch) is used in different biomedical applications to promote tissue repair.
31003033	1	110	used	used	90:93	arg2	Chitosan					73:80	Chitosan	73:80	Chitosan (Ch)	73:85	Chitosan (Ch) is used in different biomedical applications to promote tissue repair.
31003033	5	111	theme	derived	675:681	arg1	BMDM					702:705	BMDM	702:705	BMDM	702:705	For that, we assessed the NLRP3 inflammasome in bone marrow derived mouse macrophages (BMDM) and human macrophages cultured within 3D Ch scaffolds.
31003033	5	111	theme	derived	675:681	arg1	macrophages					689:699	bone marrow derived mouse macrophages	663:699	bone marrow derived mouse macrophages (BMDM)	663:706	For that, we assessed the NLRP3 inflammasome in bone marrow derived mouse macrophages (BMDM) and human macrophages cultured within 3D Ch scaffolds.
31003033	2	112	theme	biomaterial	191:201	arg1	implantation					203:214	biomaterial implantation	191:214	biomaterial implantation	191:214	However, tissue injury caused by biomaterial implantation lead to the release of danger signals that engage different inflammatory pathways on the host.
31003033	12	113	theme	nanoscale	2086:2094	arg1	products					2105:2112	nanoscale chitosan products	2086:2112	nanoscale chitosan products	2086:2112	Interestingly, our results are in contrast with studies reported in the literature that indicate that chitosan is a powerful activator of the NLRP3 inflammasome in nanoscale chitosan products.
31003033	10	114	theme	3D	1610:1611	arg1	scaffolds					1622:1630	3D chitosan scaffolds	1610:1630	3D chitosan scaffolds with different degrees of acetylation (DA)	1610:1673	STATEMENT OF SIGNIFICANCE: In this research work we have assessed the role of the NLRP3 inflammasome in the macrophage response to 3D chitosan scaffolds with different degrees of acetylation (DA).
31003033	10	115	with	scaffolds	1622:1630	arg1	degrees					1647:1653	different degrees	1637:1653	different degrees of acetylation (DA)	1637:1673	STATEMENT OF SIGNIFICANCE: In this research work we have assessed the role of the NLRP3 inflammasome in the macrophage response to 3D chitosan scaffolds with different degrees of acetylation (DA).
31003033	5	116	link	derived	675:681	arg1	BMDM					702:705	BMDM	702:705	BMDM	702:705	For that, we assessed the NLRP3 inflammasome in bone marrow derived mouse macrophages (BMDM) and human macrophages cultured within 3D Ch scaffolds.
31003033	5	116	link	derived	675:681	arg1	macrophages					689:699	bone marrow derived mouse macrophages	663:699	bone marrow derived mouse macrophages (BMDM)	663:706	For that, we assessed the NLRP3 inflammasome in bone marrow derived mouse macrophages (BMDM) and human macrophages cultured within 3D Ch scaffolds.
31003033	5	117	from	inflammasome	647:658	arg1	BMDM					702:705	BMDM	702:705	BMDM	702:705	For that, we assessed the NLRP3 inflammasome in bone marrow derived mouse macrophages (BMDM) and human macrophages cultured within 3D Ch scaffolds.
31003033	5	117	from	inflammasome	647:658	arg1	macrophages					689:699	bone marrow derived mouse macrophages	663:699	bone marrow derived mouse macrophages (BMDM)	663:706	For that, we assessed the NLRP3 inflammasome in bone marrow derived mouse macrophages (BMDM) and human macrophages cultured within 3D Ch scaffolds.
31003033	5	117	from	inflammasome	647:658	arg1	macrophages					718:728	human macrophages	712:728	human macrophages	712:728	For that, we assessed the NLRP3 inflammasome in bone marrow derived mouse macrophages (BMDM) and human macrophages cultured within 3D Ch scaffolds.
31003033	13	118	theme	phagocytosis	2213:2224	arg1	pivotal					2229:2235	pivotal	2229:2235	pivotal	2229:2235	Our studies that were performed in large scale chitosan scaffolds, stress out that the process of phagocytosis is pivotal in inflammasome assembly and activation, are rather important since they clearly illustrate the different role of the inflammasome in the biological response to large scale and nanoscale biomaterials.
31003033	13	118	theme	phagocytosis	2213:2224	arg1	process					2202:2208	the process	2198:2208	the process of phagocytosis	2198:2224	Our studies that were performed in large scale chitosan scaffolds, stress out that the process of phagocytosis is pivotal in inflammasome assembly and activation, are rather important since they clearly illustrate the different role of the inflammasome in the biological response to large scale and nanoscale biomaterials.
31003033	4	119	theme	NLRP3	586:590	arg1	inflammasome					592:603	the NLRP3 inflammasome	582:603	the NLRP3 inflammasome	582:603	Here we have studied how macroscopic biomaterials, Ch scaffolds with different chemical composition: 4% or 15% degree of acetylation (DA) modulate the activation of the NLRP3 inflammasome in vitro.
31003033	10	120	theme	different	1637:1645	arg1	degrees					1647:1653	different degrees	1637:1653	different degrees of acetylation (DA)	1637:1673	STATEMENT OF SIGNIFICANCE: In this research work we have assessed the role of the NLRP3 inflammasome in the macrophage response to 3D chitosan scaffolds with different degrees of acetylation (DA).
29973525	3	0	theme	vehicle	571:577	arg1	mice					413:416	Male ICR mice	404:416	Male ICR mice from four groups (n = 8 per group)	404:451	Male ICR mice from four groups (n = 8 per group) were orally administered KF once daily for four weeks at 0, 2.15, 4.31, and 10.76 g/kg/day and were designated as the vehicle, KF-1X, KF-2X, and KF-5X groups, respectively.
29973525	3	0	theme	vehicle	571:577	arg1	KF-2X					587:591	the vehicle, KF-1X, KF-2X, and KF-5X groups	567:609	KF-2X	587:591	Male ICR mice from four groups (n = 8 per group) were orally administered KF once daily for four weeks at 0, 2.15, 4.31, and 10.76 g/kg/day and were designated as the vehicle, KF-1X, KF-2X, and KF-5X groups, respectively.
29973525	7	1	theme	exercise	956:963	arg1	performance					965:975	exercise performance	956:975	exercise performance	956:975	In addition, anti-fatigue activity and exercise performance were evaluated on the basis of exhaustive swimming time, forelimb grip strength, and levels of serum lactate, ammonia, glucose, blood urea nitrogen (BUN), and creatine kinase (CK) after a swimming exercise.
29973525	3	2	from	groups	428:433	arg1	mice					413:416	Male ICR mice	404:416	Male ICR mice from four groups (n = 8 per group)	404:451	Male ICR mice from four groups (n = 8 per group) were orally administered KF once daily for four weeks at 0, 2.15, 4.31, and 10.76 g/kg/day and were designated as the vehicle, KF-1X, KF-2X, and KF-5X groups, respectively.
29973525	3	2	from	groups	428:433	arg1	groups					604:609	the vehicle, KF-1X, KF-2X, and KF-5X groups	567:609	groups	604:609	Male ICR mice from four groups (n = 8 per group) were orally administered KF once daily for four weeks at 0, 2.15, 4.31, and 10.76 g/kg/day and were designated as the vehicle, KF-1X, KF-2X, and KF-5X groups, respectively.
29973525	3	2	from	groups	428:433	arg1	KF-2X					587:591	the vehicle, KF-1X, KF-2X, and KF-5X groups	567:609	KF-2X	587:591	Male ICR mice from four groups (n = 8 per group) were orally administered KF once daily for four weeks at 0, 2.15, 4.31, and 10.76 g/kg/day and were designated as the vehicle, KF-1X, KF-2X, and KF-5X groups, respectively.
29973525	2	3	theme	gut	255:257	arg1	microbiota					259:268	the gut microbiota	251:268	the gut microbiota	251:268	Furthermore, the composition of the gut microbiota is related to health benefits in the host; therefore, the study also investigated the effect of KF on the gut microbiota composition.
29973525	1	4	theme	kefir	191:195	arg1	effect					181:186	the potential beneficial effect	156:186	the potential beneficial effect of kefir (KF) against fatigue	156:216	The present study evaluated the potential beneficial effect of kefir (KF) against fatigue.
29973525	6	5	theme	KF-2X	811:815	arg1	groups					827:832	The KF-2X and KF-5X groups	807:832	The KF-2X and KF-5X groups	807:832	The KF-2X and KF-5X groups showed a decreased Firmicutes/Bacteroidetes ratio compared with the vehicle group.
29973525	3	6	theme	daily	486:490	arg1	KF					478:479	orally administered KF	458:479	orally administered KF once daily for four weeks at 0, 2.15, 4.31, and 10.76 g/kg/day	458:542	Male ICR mice from four groups (n = 8 per group) were orally administered KF once daily for four weeks at 0, 2.15, 4.31, and 10.76 g/kg/day and were designated as the vehicle, KF-1X, KF-2X, and KF-5X groups, respectively.
29973525	7	7	theme	swimming	1019:1026	arg1	time					1028:1031	exhaustive swimming time	1008:1031	exhaustive swimming time	1008:1031	In addition, anti-fatigue activity and exercise performance were evaluated on the basis of exhaustive swimming time, forelimb grip strength, and levels of serum lactate, ammonia, glucose, blood urea nitrogen (BUN), and creatine kinase (CK) after a swimming exercise.
29973525	11	8	theme	physical	1766:1773	arg1	fatigue					1775:1781	physical fatigue	1766:1781	physical fatigue	1766:1781	Thus, KF supplementation can alter the gut microbiota composition, improve performance, and combat physical fatigue.
29973525	5	9	theme	significant	711:721	arg1	clustering					723:732	a significant clustering	709:732	a significant clustering of cecum	709:741	The results showed a significant clustering of cecum after treatment in the vehicle, KF-1X, KF-2X, and KF-5X groups.
29973525	9	10	theme	lactate	1481:1487	arg1	levels					1511:1516	serum lactate, ammonia, BUN, and CK levels	1475:1516	serum lactate, ammonia, BUN, and CK levels	1475:1516	KF supplementation also decreased serum lactate, ammonia, BUN, and CK levels after the swimming test.
29973525	8	11	theme	KF-5X	1239:1243	arg1	groups					1245:1250	the KF-1X, KF-2X, and KF-5X groups	1217:1250	the KF-1X, KF-2X, and KF-5X groups	1217:1250	The exhaustive swimming time for the KF-1X, KF-2X, and KF-5X groups was significantly longer than that for the vehicle group, and the forelimb grip strength of the KF-1X, KF-2X, and KF-5X groups was also significantly higher than that of the vehicle group.
29973525	7	12	theme	swimming	1165:1172	arg1	exercise					1174:1181	a swimming exercise	1163:1181	a swimming exercise	1163:1181	In addition, anti-fatigue activity and exercise performance were evaluated on the basis of exhaustive swimming time, forelimb grip strength, and levels of serum lactate, ammonia, glucose, blood urea nitrogen (BUN), and creatine kinase (CK) after a swimming exercise.
29973525	5	13	theme	KF-1X	775:779	arg1	groups					799:804	the vehicle, KF-1X, KF-2X, and KF-5X groups	762:804	the vehicle, KF-1X, KF-2X, and KF-5X groups	762:804	The results showed a significant clustering of cecum after treatment in the vehicle, KF-1X, KF-2X, and KF-5X groups.
29973525	9	14	theme	ammonia	1490:1496	arg1	levels					1511:1516	serum lactate, ammonia, BUN, and CK levels	1475:1516	serum lactate, ammonia, BUN, and CK levels	1475:1516	KF supplementation also decreased serum lactate, ammonia, BUN, and CK levels after the swimming test.
29973525	0	15	from	Performance	107:117	arg1	Mice					122:125	Mice	122:125	Mice	122:125	Kefir Supplementation Modifies Gut Microbiota Composition, Reduces Physical Fatigue, and Improves Exercise Performance in Mice.
29973525	5	16	theme	KF-2X	782:786	arg1	groups					799:804	the vehicle, KF-1X, KF-2X, and KF-5X groups	762:804	the vehicle, KF-1X, KF-2X, and KF-5X groups	762:804	The results showed a significant clustering of cecum after treatment in the vehicle, KF-1X, KF-2X, and KF-5X groups.
29973525	9	17	theme	BUN	1499:1501	arg1	levels					1511:1516	serum lactate, ammonia, BUN, and CK levels	1475:1516	serum lactate, ammonia, BUN, and CK levels	1475:1516	KF supplementation also decreased serum lactate, ammonia, BUN, and CK levels after the swimming test.
29973525	7	18	theme	lactate	1078:1084	arg1	time					1028:1031	exhaustive swimming time	1008:1031	exhaustive swimming time	1008:1031	In addition, anti-fatigue activity and exercise performance were evaluated on the basis of exhaustive swimming time, forelimb grip strength, and levels of serum lactate, ammonia, glucose, blood urea nitrogen (BUN), and creatine kinase (CK) after a swimming exercise.
29973525	7	18	theme	lactate	1078:1084	arg1	strength					1048:1055	forelimb grip strength	1034:1055	forelimb grip strength	1034:1055	In addition, anti-fatigue activity and exercise performance were evaluated on the basis of exhaustive swimming time, forelimb grip strength, and levels of serum lactate, ammonia, glucose, blood urea nitrogen (BUN), and creatine kinase (CK) after a swimming exercise.
29973525	7	18	theme	lactate	1078:1084	arg1	levels					1062:1067	levels	1062:1067	levels of serum lactate, ammonia, glucose, blood urea nitrogen (BUN), and creatine kinase (CK)	1062:1155	In addition, anti-fatigue activity and exercise performance were evaluated on the basis of exhaustive swimming time, forelimb grip strength, and levels of serum lactate, ammonia, glucose, blood urea nitrogen (BUN), and creatine kinase (CK) after a swimming exercise.
29973525	10	19	theme	tissue	1552:1557	arg1	source					1597:1602	an important energy source	1577:1602	an important energy source for exercise	1577:1615	However, tissue glycogen content, an important energy source for exercise, increased significantly with KF supplementation.
29973525	10	19	theme	tissue	1552:1557	arg1	content					1568:1574	tissue glycogen content	1552:1574	tissue glycogen content	1552:1574	However, tissue glycogen content, an important energy source for exercise, increased significantly with KF supplementation.
29973525	6	20	theme	decreased	843:851	arg1	ratio					878:882	a decreased Firmicutes/Bacteroidetes ratio	841:882	a decreased Firmicutes/Bacteroidetes ratio	841:882	The KF-2X and KF-5X groups showed a decreased Firmicutes/Bacteroidetes ratio compared with the vehicle group.
29973525	11	21	theme	gut	1706:1708	arg1	composition					1721:1731	the gut microbiota composition	1702:1731	the gut microbiota composition	1702:1731	Thus, KF supplementation can alter the gut microbiota composition, improve performance, and combat physical fatigue.
29973525	1	22	theme	potential	160:168	arg1	effect					181:186	the potential beneficial effect	156:186	the potential beneficial effect of kefir (KF) against fatigue	156:216	The present study evaluated the potential beneficial effect of kefir (KF) against fatigue.
29973525	4	23	theme	gut	630:632	arg1	microbiota					634:643	The gut microbiota	626:643	The gut microbiota	626:643	The gut microbiota was analyzed using 16S rRNA gene sequencing.
29973525	6	24	theme	KF-5X	821:825	arg1	groups					827:832	The KF-2X and KF-5X groups	807:832	The KF-2X and KF-5X groups	807:832	The KF-2X and KF-5X groups showed a decreased Firmicutes/Bacteroidetes ratio compared with the vehicle group.
29973525	7	25	theme	strength	1048:1055	arg1	basis					999:1003	the basis	995:1003	the basis of exhaustive swimming time, forelimb grip strength, and levels of serum lactate, ammonia, glucose, blood urea nitrogen (BUN), and creatine kinase (CK)	995:1155	In addition, anti-fatigue activity and exercise performance were evaluated on the basis of exhaustive swimming time, forelimb grip strength, and levels of serum lactate, ammonia, glucose, blood urea nitrogen (BUN), and creatine kinase (CK) after a swimming exercise.
29973525	1	26	theme	present	132:138	arg1	study					140:144	The present study	128:144	The present study	128:144	The present study evaluated the potential beneficial effect of kefir (KF) against fatigue.
29973525	2	27	dep	host	307:310	arg1	investigated					339:350	investigated	339:350	investigated the effect of KF on the gut microbiota composition	339:401	Furthermore, the composition of the gut microbiota is related to health benefits in the host; therefore, the study also investigated the effect of KF on the gut microbiota composition.
29973525	0	28	theme	Kefir	0:4	arg1	Supplementation					6:20	Kefir Supplementation	0:20	Kefir Supplementation	0:20	Kefir Supplementation Modifies Gut Microbiota Composition, Reduces Physical Fatigue, and Improves Exercise Performance in Mice.
29973525	7	29	theme	creatine	1136:1143	arg1	CK					1153:1154	CK	1153:1154	CK	1153:1154	In addition, anti-fatigue activity and exercise performance were evaluated on the basis of exhaustive swimming time, forelimb grip strength, and levels of serum lactate, ammonia, glucose, blood urea nitrogen (BUN), and creatine kinase (CK) after a swimming exercise.
29973525	7	29	theme	creatine	1136:1143	arg1	kinase					1145:1150	creatine kinase	1136:1150	creatine kinase (CK)	1136:1155	In addition, anti-fatigue activity and exercise performance were evaluated on the basis of exhaustive swimming time, forelimb grip strength, and levels of serum lactate, ammonia, glucose, blood urea nitrogen (BUN), and creatine kinase (CK) after a swimming exercise.
29973525	8	30	theme	forelimb	1318:1325	arg1	strength					1332:1339	the forelimb grip strength	1314:1339	the forelimb grip strength of the KF-1X, KF-2X, and KF-5X groups	1314:1377	The exhaustive swimming time for the KF-1X, KF-2X, and KF-5X groups was significantly longer than that for the vehicle group, and the forelimb grip strength of the KF-1X, KF-2X, and KF-5X groups was also significantly higher than that of the vehicle group.
29973525	8	30	theme	forelimb	1318:1325	arg1	higher					1402:1407	higher	1402:1407	higher	1402:1407	The exhaustive swimming time for the KF-1X, KF-2X, and KF-5X groups was significantly longer than that for the vehicle group, and the forelimb grip strength of the KF-1X, KF-2X, and KF-5X groups was also significantly higher than that of the vehicle group.
29973525	2	31	theme	gut	376:378	arg1	composition					391:401	the gut microbiota composition	372:401	the gut microbiota composition	372:401	Furthermore, the composition of the gut microbiota is related to health benefits in the host; therefore, the study also investigated the effect of KF on the gut microbiota composition.
29973525	7	32	theme	time	1028:1031	arg1	basis					999:1003	the basis	995:1003	the basis of exhaustive swimming time, forelimb grip strength, and levels of serum lactate, ammonia, glucose, blood urea nitrogen (BUN), and creatine kinase (CK)	995:1155	In addition, anti-fatigue activity and exercise performance were evaluated on the basis of exhaustive swimming time, forelimb grip strength, and levels of serum lactate, ammonia, glucose, blood urea nitrogen (BUN), and creatine kinase (CK) after a swimming exercise.
29973525	6	33	theme	vehicle	902:908	arg1	group					910:914	the vehicle group	898:914	the vehicle group	898:914	The KF-2X and KF-5X groups showed a decreased Firmicutes/Bacteroidetes ratio compared with the vehicle group.
29973525	7	34	theme	forelimb	1034:1041	arg1	strength					1048:1055	forelimb grip strength	1034:1055	forelimb grip strength	1034:1055	In addition, anti-fatigue activity and exercise performance were evaluated on the basis of exhaustive swimming time, forelimb grip strength, and levels of serum lactate, ammonia, glucose, blood urea nitrogen (BUN), and creatine kinase (CK) after a swimming exercise.
29973525	8	35	theme	swimming	1199:1206	arg1	time					1208:1211	The exhaustive swimming time	1184:1211	The exhaustive swimming time for the KF-1X, KF-2X, and KF-5X groups	1184:1250	The exhaustive swimming time for the KF-1X, KF-2X, and KF-5X groups was significantly longer than that for the vehicle group, and the forelimb grip strength of the KF-1X, KF-2X, and KF-5X groups was also significantly higher than that of the vehicle group.
29973525	8	35	theme	swimming	1199:1206	arg1	longer					1270:1275	longer	1270:1275	longer	1270:1275	The exhaustive swimming time for the KF-1X, KF-2X, and KF-5X groups was significantly longer than that for the vehicle group, and the forelimb grip strength of the KF-1X, KF-2X, and KF-5X groups was also significantly higher than that of the vehicle group.
29973525	3	36	theme	ICR	409:411	arg1	mice					413:416	Male ICR mice	404:416	Male ICR mice from four groups (n = 8 per group)	404:451	Male ICR mice from four groups (n = 8 per group) were orally administered KF once daily for four weeks at 0, 2.15, 4.31, and 10.76 g/kg/day and were designated as the vehicle, KF-1X, KF-2X, and KF-5X groups, respectively.
29973525	3	36	theme	ICR	409:411	arg1	groups					604:609	the vehicle, KF-1X, KF-2X, and KF-5X groups	567:609	groups	604:609	Male ICR mice from four groups (n = 8 per group) were orally administered KF once daily for four weeks at 0, 2.15, 4.31, and 10.76 g/kg/day and were designated as the vehicle, KF-1X, KF-2X, and KF-5X groups, respectively.
29973525	3	36	theme	ICR	409:411	arg1	KF-2X					587:591	the vehicle, KF-1X, KF-2X, and KF-5X groups	567:609	KF-2X	587:591	Male ICR mice from four groups (n = 8 per group) were orally administered KF once daily for four weeks at 0, 2.15, 4.31, and 10.76 g/kg/day and were designated as the vehicle, KF-1X, KF-2X, and KF-5X groups, respectively.
29973525	0	37	theme	Microbiota	35:44	arg1	Composition					46:56	Gut Microbiota Composition	31:56	Gut Microbiota Composition	31:56	Kefir Supplementation Modifies Gut Microbiota Composition, Reduces Physical Fatigue, and Improves Exercise Performance in Mice.
29973525	3	38	dep	KF-2X	587:591	arg1	KF-1X					580:584	the vehicle, KF-1X, KF-2X, and KF-5X groups	567:609	KF-1X	580:584	Male ICR mice from four groups (n = 8 per group) were orally administered KF once daily for four weeks at 0, 2.15, 4.31, and 10.76 g/kg/day and were designated as the vehicle, KF-1X, KF-2X, and KF-5X groups, respectively.
29973525	7	39	theme	exhaustive	1008:1017	arg1	time					1028:1031	exhaustive swimming time	1008:1031	exhaustive swimming time	1008:1031	In addition, anti-fatigue activity and exercise performance were evaluated on the basis of exhaustive swimming time, forelimb grip strength, and levels of serum lactate, ammonia, glucose, blood urea nitrogen (BUN), and creatine kinase (CK) after a swimming exercise.
29973525	0	40	theme	Physical	67:74	arg1	Fatigue					76:82	Physical Fatigue	67:82	Physical Fatigue	67:82	Kefir Supplementation Modifies Gut Microbiota Composition, Reduces Physical Fatigue, and Improves Exercise Performance in Mice.
29973525	10	41	theme	energy	1590:1595	arg1	source					1597:1602	an important energy source	1577:1602	an important energy source for exercise	1577:1615	However, tissue glycogen content, an important energy source for exercise, increased significantly with KF supplementation.
29973525	10	41	theme	energy	1590:1595	arg1	content					1568:1574	tissue glycogen content	1552:1574	tissue glycogen content	1552:1574	However, tissue glycogen content, an important energy source for exercise, increased significantly with KF supplementation.
29973525	7	42	theme	nitrogen	1116:1123	arg1	time					1028:1031	exhaustive swimming time	1008:1031	exhaustive swimming time	1008:1031	In addition, anti-fatigue activity and exercise performance were evaluated on the basis of exhaustive swimming time, forelimb grip strength, and levels of serum lactate, ammonia, glucose, blood urea nitrogen (BUN), and creatine kinase (CK) after a swimming exercise.
29973525	7	42	theme	nitrogen	1116:1123	arg1	strength					1048:1055	forelimb grip strength	1034:1055	forelimb grip strength	1034:1055	In addition, anti-fatigue activity and exercise performance were evaluated on the basis of exhaustive swimming time, forelimb grip strength, and levels of serum lactate, ammonia, glucose, blood urea nitrogen (BUN), and creatine kinase (CK) after a swimming exercise.
29973525	7	42	theme	nitrogen	1116:1123	arg1	levels					1062:1067	levels	1062:1067	levels of serum lactate, ammonia, glucose, blood urea nitrogen (BUN), and creatine kinase (CK)	1062:1155	In addition, anti-fatigue activity and exercise performance were evaluated on the basis of exhaustive swimming time, forelimb grip strength, and levels of serum lactate, ammonia, glucose, blood urea nitrogen (BUN), and creatine kinase (CK) after a swimming exercise.
29973525	11	43	theme	microbiota	1710:1719	arg1	composition					1721:1731	the gut microbiota composition	1702:1731	the gut microbiota composition	1702:1731	Thus, KF supplementation can alter the gut microbiota composition, improve performance, and combat physical fatigue.
29973525	8	44	theme	KF-1X	1221:1225	arg1	groups					1245:1250	the KF-1X, KF-2X, and KF-5X groups	1217:1250	the KF-1X, KF-2X, and KF-5X groups	1217:1250	The exhaustive swimming time for the KF-1X, KF-2X, and KF-5X groups was significantly longer than that for the vehicle group, and the forelimb grip strength of the KF-1X, KF-2X, and KF-5X groups was also significantly higher than that of the vehicle group.
29973525	4	45	theme	gene	673:676	arg1	sequencing					678:687	16S rRNA gene sequencing	664:687	16S rRNA gene sequencing	664:687	The gut microbiota was analyzed using 16S rRNA gene sequencing.
29973525	7	46	theme	blood	1105:1109	arg1	BUN					1126:1128	BUN	1126:1128	BUN	1126:1128	In addition, anti-fatigue activity and exercise performance were evaluated on the basis of exhaustive swimming time, forelimb grip strength, and levels of serum lactate, ammonia, glucose, blood urea nitrogen (BUN), and creatine kinase (CK) after a swimming exercise.
29973525	7	46	theme	blood	1105:1109	arg1	nitrogen					1116:1123	blood urea nitrogen	1105:1123	blood urea nitrogen (BUN)	1105:1129	In addition, anti-fatigue activity and exercise performance were evaluated on the basis of exhaustive swimming time, forelimb grip strength, and levels of serum lactate, ammonia, glucose, blood urea nitrogen (BUN), and creatine kinase (CK) after a swimming exercise.
29973525	8	47	theme	KF-2X	1228:1232	arg1	groups					1245:1250	the KF-1X, KF-2X, and KF-5X groups	1217:1250	the KF-1X, KF-2X, and KF-5X groups	1217:1250	The exhaustive swimming time for the KF-1X, KF-2X, and KF-5X groups was significantly longer than that for the vehicle group, and the forelimb grip strength of the KF-1X, KF-2X, and KF-5X groups was also significantly higher than that of the vehicle group.
29973525	7	48	theme	glucose	1096:1102	arg1	time					1028:1031	exhaustive swimming time	1008:1031	exhaustive swimming time	1008:1031	In addition, anti-fatigue activity and exercise performance were evaluated on the basis of exhaustive swimming time, forelimb grip strength, and levels of serum lactate, ammonia, glucose, blood urea nitrogen (BUN), and creatine kinase (CK) after a swimming exercise.
29973525	7	48	theme	glucose	1096:1102	arg1	strength					1048:1055	forelimb grip strength	1034:1055	forelimb grip strength	1034:1055	In addition, anti-fatigue activity and exercise performance were evaluated on the basis of exhaustive swimming time, forelimb grip strength, and levels of serum lactate, ammonia, glucose, blood urea nitrogen (BUN), and creatine kinase (CK) after a swimming exercise.
29973525	7	48	theme	glucose	1096:1102	arg1	levels					1062:1067	levels	1062:1067	levels of serum lactate, ammonia, glucose, blood urea nitrogen (BUN), and creatine kinase (CK)	1062:1155	In addition, anti-fatigue activity and exercise performance were evaluated on the basis of exhaustive swimming time, forelimb grip strength, and levels of serum lactate, ammonia, glucose, blood urea nitrogen (BUN), and creatine kinase (CK) after a swimming exercise.
29973525	8	49	theme	KF-5X	1366:1370	arg1	groups					1372:1377	the KF-1X, KF-2X, and KF-5X groups	1344:1377	groups	1372:1377	The exhaustive swimming time for the KF-1X, KF-2X, and KF-5X groups was significantly longer than that for the vehicle group, and the forelimb grip strength of the KF-1X, KF-2X, and KF-5X groups was also significantly higher than that of the vehicle group.
29973525	10	50	theme	KF	1647:1648	arg1	supplementation					1650:1664	KF supplementation	1647:1664	KF supplementation	1647:1664	However, tissue glycogen content, an important energy source for exercise, increased significantly with KF supplementation.
29973525	8	51	theme	vehicle	1426:1432	arg1	group					1434:1438	the vehicle group	1422:1438	the vehicle group	1422:1438	The exhaustive swimming time for the KF-1X, KF-2X, and KF-5X groups was significantly longer than that for the vehicle group, and the forelimb grip strength of the KF-1X, KF-2X, and KF-5X groups was also significantly higher than that of the vehicle group.
29973525	7	52	theme	ammonia	1087:1093	arg1	time					1028:1031	exhaustive swimming time	1008:1031	exhaustive swimming time	1008:1031	In addition, anti-fatigue activity and exercise performance were evaluated on the basis of exhaustive swimming time, forelimb grip strength, and levels of serum lactate, ammonia, glucose, blood urea nitrogen (BUN), and creatine kinase (CK) after a swimming exercise.
29973525	7	52	theme	ammonia	1087:1093	arg1	strength					1048:1055	forelimb grip strength	1034:1055	forelimb grip strength	1034:1055	In addition, anti-fatigue activity and exercise performance were evaluated on the basis of exhaustive swimming time, forelimb grip strength, and levels of serum lactate, ammonia, glucose, blood urea nitrogen (BUN), and creatine kinase (CK) after a swimming exercise.
29973525	7	52	theme	ammonia	1087:1093	arg1	levels					1062:1067	levels	1062:1067	levels of serum lactate, ammonia, glucose, blood urea nitrogen (BUN), and creatine kinase (CK)	1062:1155	In addition, anti-fatigue activity and exercise performance were evaluated on the basis of exhaustive swimming time, forelimb grip strength, and levels of serum lactate, ammonia, glucose, blood urea nitrogen (BUN), and creatine kinase (CK) after a swimming exercise.
29973525	3	53	theme	administered	465:476	arg1	KF					478:479	orally administered KF	458:479	orally administered KF once daily for four weeks at 0, 2.15, 4.31, and 10.76 g/kg/day	458:542	Male ICR mice from four groups (n = 8 per group) were orally administered KF once daily for four weeks at 0, 2.15, 4.31, and 10.76 g/kg/day and were designated as the vehicle, KF-1X, KF-2X, and KF-5X groups, respectively.
29973525	3	54	from	g/kg/day	535:542	arg1	weeks					501:505	four weeks	496:505	four weeks at 0, 2.15, 4.31, and 10.76 g/kg/day	496:542	Male ICR mice from four groups (n = 8 per group) were orally administered KF once daily for four weeks at 0, 2.15, 4.31, and 10.76 g/kg/day and were designated as the vehicle, KF-1X, KF-2X, and KF-5X groups, respectively.
29973525	3	55	theme	KF-5X	598:602	arg1	mice					413:416	Male ICR mice	404:416	Male ICR mice from four groups (n = 8 per group)	404:451	Male ICR mice from four groups (n = 8 per group) were orally administered KF once daily for four weeks at 0, 2.15, 4.31, and 10.76 g/kg/day and were designated as the vehicle, KF-1X, KF-2X, and KF-5X groups, respectively.
29973525	3	55	theme	KF-5X	598:602	arg1	groups					604:609	the vehicle, KF-1X, KF-2X, and KF-5X groups	567:609	groups	604:609	Male ICR mice from four groups (n = 8 per group) were orally administered KF once daily for four weeks at 0, 2.15, 4.31, and 10.76 g/kg/day and were designated as the vehicle, KF-1X, KF-2X, and KF-5X groups, respectively.
29973525	2	56	theme	microbiota	259:268	arg1	composition					236:246	the composition	232:246	the composition of the gut microbiota	232:268	Furthermore, the composition of the gut microbiota is related to health benefits in the host; therefore, the study also investigated the effect of KF on the gut microbiota composition.
29973525	2	56	theme	microbiota	259:268	arg1	related					273:279	related	273:279	related	273:279	Furthermore, the composition of the gut microbiota is related to health benefits in the host; therefore, the study also investigated the effect of KF on the gut microbiota composition.
29973525	1	57	theme	beneficial	170:179	arg1	effect					181:186	the potential beneficial effect	156:186	the potential beneficial effect of kefir (KF) against fatigue	156:216	The present study evaluated the potential beneficial effect of kefir (KF) against fatigue.
29973525	7	58	theme	anti-fatigue	930:941	arg1	activity					943:950	anti-fatigue activity	930:950	anti-fatigue activity	930:950	In addition, anti-fatigue activity and exercise performance were evaluated on the basis of exhaustive swimming time, forelimb grip strength, and levels of serum lactate, ammonia, glucose, blood urea nitrogen (BUN), and creatine kinase (CK) after a swimming exercise.
29973525	5	59	theme	cecum	737:741	arg1	clustering					723:732	a significant clustering	709:732	a significant clustering of cecum	709:741	The results showed a significant clustering of cecum after treatment in the vehicle, KF-1X, KF-2X, and KF-5X groups.
29973525	9	60	theme	KF	1441:1442	arg1	supplementation					1444:1458	KF supplementation	1441:1458	KF supplementation	1441:1458	KF supplementation also decreased serum lactate, ammonia, BUN, and CK levels after the swimming test.
29973525	2	61	theme	health	284:289	arg1	benefits					291:298	health benefits	284:298	health benefits in the host; therefore, the study also investigated the effect of KF on the gut microbiota composition	284:401	Furthermore, the composition of the gut microbiota is related to health benefits in the host; therefore, the study also investigated the effect of KF on the gut microbiota composition.
29973525	5	62	theme	vehicle	766:772	arg1	groups					799:804	the vehicle, KF-1X, KF-2X, and KF-5X groups	762:804	the vehicle, KF-1X, KF-2X, and KF-5X groups	762:804	The results showed a significant clustering of cecum after treatment in the vehicle, KF-1X, KF-2X, and KF-5X groups.
29973525	9	63	theme	serum	1475:1479	arg1	lactate					1481:1487	serum lactate	1475:1487	serum lactate	1475:1487	KF supplementation also decreased serum lactate, ammonia, BUN, and CK levels after the swimming test.
29973525	7	64	theme	serum	1072:1076	arg1	lactate					1078:1084	serum lactate	1072:1084	serum lactate	1072:1084	In addition, anti-fatigue activity and exercise performance were evaluated on the basis of exhaustive swimming time, forelimb grip strength, and levels of serum lactate, ammonia, glucose, blood urea nitrogen (BUN), and creatine kinase (CK) after a swimming exercise.
29973525	10	65	theme	glycogen	1559:1566	arg1	source					1597:1602	an important energy source	1577:1602	an important energy source for exercise	1577:1615	However, tissue glycogen content, an important energy source for exercise, increased significantly with KF supplementation.
29973525	10	65	theme	glycogen	1559:1566	arg1	content					1568:1574	tissue glycogen content	1552:1574	tissue glycogen content	1552:1574	However, tissue glycogen content, an important energy source for exercise, increased significantly with KF supplementation.
29973525	6	66	theme	Firmicutes/Bacteroidetes	853:876	arg1	ratio					878:882	a decreased Firmicutes/Bacteroidetes ratio	841:882	a decreased Firmicutes/Bacteroidetes ratio	841:882	The KF-2X and KF-5X groups showed a decreased Firmicutes/Bacteroidetes ratio compared with the vehicle group.
29973525	7	67	theme	levels	1062:1067	arg1	basis					999:1003	the basis	995:1003	the basis of exhaustive swimming time, forelimb grip strength, and levels of serum lactate, ammonia, glucose, blood urea nitrogen (BUN), and creatine kinase (CK)	995:1155	In addition, anti-fatigue activity and exercise performance were evaluated on the basis of exhaustive swimming time, forelimb grip strength, and levels of serum lactate, ammonia, glucose, blood urea nitrogen (BUN), and creatine kinase (CK) after a swimming exercise.
29973525	9	68	theme	CK	1508:1509	arg1	levels					1511:1516	serum lactate, ammonia, BUN, and CK levels	1475:1516	serum lactate, ammonia, BUN, and CK levels	1475:1516	KF supplementation also decreased serum lactate, ammonia, BUN, and CK levels after the swimming test.
29973525	8	69	theme	vehicle	1295:1301	arg1	group					1303:1307	the vehicle group	1291:1307	the vehicle group	1291:1307	The exhaustive swimming time for the KF-1X, KF-2X, and KF-5X groups was significantly longer than that for the vehicle group, and the forelimb grip strength of the KF-1X, KF-2X, and KF-5X groups was also significantly higher than that of the vehicle group.
29973525	0	70	theme	Exercise	98:105	arg1	Performance					107:117	Exercise Performance	98:117	Exercise Performance in Mice	98:125	Kefir Supplementation Modifies Gut Microbiota Composition, Reduces Physical Fatigue, and Improves Exercise Performance in Mice.
29973525	3	71	dep	groups	428:433	arg1	n					436:436	n = 8	436:440	n = 8 per group	436:450	Male ICR mice from four groups (n = 8 per group) were orally administered KF once daily for four weeks at 0, 2.15, 4.31, and 10.76 g/kg/day and were designated as the vehicle, KF-1X, KF-2X, and KF-5X groups, respectively.
29973525	2	72	from	benefits	291:298	arg1	host					307:310	the host	303:310	the host; therefore, the study also investigated the effect of KF on the gut microbiota composition	303:401	Furthermore, the composition of the gut microbiota is related to health benefits in the host; therefore, the study also investigated the effect of KF on the gut microbiota composition.
29973525	7	73	theme	grip	1043:1046	arg1	strength					1048:1055	forelimb grip strength	1034:1055	forelimb grip strength	1034:1055	In addition, anti-fatigue activity and exercise performance were evaluated on the basis of exhaustive swimming time, forelimb grip strength, and levels of serum lactate, ammonia, glucose, blood urea nitrogen (BUN), and creatine kinase (CK) after a swimming exercise.
29973525	7	74	theme	kinase	1145:1150	arg1	time					1028:1031	exhaustive swimming time	1008:1031	exhaustive swimming time	1008:1031	In addition, anti-fatigue activity and exercise performance were evaluated on the basis of exhaustive swimming time, forelimb grip strength, and levels of serum lactate, ammonia, glucose, blood urea nitrogen (BUN), and creatine kinase (CK) after a swimming exercise.
29973525	7	74	theme	kinase	1145:1150	arg1	strength					1048:1055	forelimb grip strength	1034:1055	forelimb grip strength	1034:1055	In addition, anti-fatigue activity and exercise performance were evaluated on the basis of exhaustive swimming time, forelimb grip strength, and levels of serum lactate, ammonia, glucose, blood urea nitrogen (BUN), and creatine kinase (CK) after a swimming exercise.
29973525	7	74	theme	kinase	1145:1150	arg1	levels					1062:1067	levels	1062:1067	levels of serum lactate, ammonia, glucose, blood urea nitrogen (BUN), and creatine kinase (CK)	1062:1155	In addition, anti-fatigue activity and exercise performance were evaluated on the basis of exhaustive swimming time, forelimb grip strength, and levels of serum lactate, ammonia, glucose, blood urea nitrogen (BUN), and creatine kinase (CK) after a swimming exercise.
29973525	9	75	theme	swimming	1528:1535	arg1	test					1537:1540	the swimming test	1524:1540	the swimming test	1524:1540	KF supplementation also decreased serum lactate, ammonia, BUN, and CK levels after the swimming test.
29973525	3	76	theme	Male	404:407	arg1	mice					413:416	Male ICR mice	404:416	Male ICR mice from four groups (n = 8 per group)	404:451	Male ICR mice from four groups (n = 8 per group) were orally administered KF once daily for four weeks at 0, 2.15, 4.31, and 10.76 g/kg/day and were designated as the vehicle, KF-1X, KF-2X, and KF-5X groups, respectively.
29973525	3	76	theme	Male	404:407	arg1	groups					604:609	the vehicle, KF-1X, KF-2X, and KF-5X groups	567:609	groups	604:609	Male ICR mice from four groups (n = 8 per group) were orally administered KF once daily for four weeks at 0, 2.15, 4.31, and 10.76 g/kg/day and were designated as the vehicle, KF-1X, KF-2X, and KF-5X groups, respectively.
29973525	3	76	theme	Male	404:407	arg1	KF-2X					587:591	the vehicle, KF-1X, KF-2X, and KF-5X groups	567:609	KF-2X	587:591	Male ICR mice from four groups (n = 8 per group) were orally administered KF once daily for four weeks at 0, 2.15, 4.31, and 10.76 g/kg/day and were designated as the vehicle, KF-1X, KF-2X, and KF-5X groups, respectively.
29973525	8	77	theme	grip	1327:1330	arg1	strength					1332:1339	the forelimb grip strength	1314:1339	the forelimb grip strength of the KF-1X, KF-2X, and KF-5X groups	1314:1377	The exhaustive swimming time for the KF-1X, KF-2X, and KF-5X groups was significantly longer than that for the vehicle group, and the forelimb grip strength of the KF-1X, KF-2X, and KF-5X groups was also significantly higher than that of the vehicle group.
29973525	8	77	theme	grip	1327:1330	arg1	higher					1402:1407	higher	1402:1407	higher	1402:1407	The exhaustive swimming time for the KF-1X, KF-2X, and KF-5X groups was significantly longer than that for the vehicle group, and the forelimb grip strength of the KF-1X, KF-2X, and KF-5X groups was also significantly higher than that of the vehicle group.
29973525	0	78	theme	Gut	31:33	arg1	Composition					46:56	Gut Microbiota Composition	31:56	Gut Microbiota Composition	31:56	Kefir Supplementation Modifies Gut Microbiota Composition, Reduces Physical Fatigue, and Improves Exercise Performance in Mice.
29973525	4	79	theme	rRNA	668:671	arg1	sequencing					678:687	16S rRNA gene sequencing	664:687	16S rRNA gene sequencing	664:687	The gut microbiota was analyzed using 16S rRNA gene sequencing.
29973525	5	80	theme	KF-5X	793:797	arg1	groups					799:804	the vehicle, KF-1X, KF-2X, and KF-5X groups	762:804	the vehicle, KF-1X, KF-2X, and KF-5X groups	762:804	The results showed a significant clustering of cecum after treatment in the vehicle, KF-1X, KF-2X, and KF-5X groups.
29973525	2	81	theme	microbiota	380:389	arg1	composition					391:401	the gut microbiota composition	372:401	the gut microbiota composition	372:401	Furthermore, the composition of the gut microbiota is related to health benefits in the host; therefore, the study also investigated the effect of KF on the gut microbiota composition.
29973525	11	82	theme	KF	1673:1674	arg1	supplementation					1676:1690	KF supplementation	1673:1690	KF supplementation	1673:1690	Thus, KF supplementation can alter the gut microbiota composition, improve performance, and combat physical fatigue.
29973525	8	83	theme	KF-1X	1348:1352	arg1	KF-2X					1355:1359	the KF-1X, KF-2X, and KF-5X groups	1344:1377	KF-2X	1355:1359	The exhaustive swimming time for the KF-1X, KF-2X, and KF-5X groups was significantly longer than that for the vehicle group, and the forelimb grip strength of the KF-1X, KF-2X, and KF-5X groups was also significantly higher than that of the vehicle group.
29973525	10	84	theme	important	1580:1588	arg1	source					1597:1602	an important energy source	1577:1602	an important energy source for exercise	1577:1615	However, tissue glycogen content, an important energy source for exercise, increased significantly with KF supplementation.
29973525	10	84	theme	important	1580:1588	arg1	content					1568:1574	tissue glycogen content	1552:1574	tissue glycogen content	1552:1574	However, tissue glycogen content, an important energy source for exercise, increased significantly with KF supplementation.
29973525	8	85	theme	exhaustive	1188:1197	arg1	time					1208:1211	The exhaustive swimming time	1184:1211	The exhaustive swimming time for the KF-1X, KF-2X, and KF-5X groups	1184:1250	The exhaustive swimming time for the KF-1X, KF-2X, and KF-5X groups was significantly longer than that for the vehicle group, and the forelimb grip strength of the KF-1X, KF-2X, and KF-5X groups was also significantly higher than that of the vehicle group.
29973525	8	85	theme	exhaustive	1188:1197	arg1	longer					1270:1275	longer	1270:1275	longer	1270:1275	The exhaustive swimming time for the KF-1X, KF-2X, and KF-5X groups was significantly longer than that for the vehicle group, and the forelimb grip strength of the KF-1X, KF-2X, and KF-5X groups was also significantly higher than that of the vehicle group.
29973525	2	86	theme	KF	366:367	arg1	effect					356:361	the effect	352:361	the effect of KF on the gut microbiota composition	352:401	Furthermore, the composition of the gut microbiota is related to health benefits in the host; therefore, the study also investigated the effect of KF on the gut microbiota composition.
29973525	7	87	theme	urea	1111:1114	arg1	BUN					1126:1128	BUN	1126:1128	BUN	1126:1128	In addition, anti-fatigue activity and exercise performance were evaluated on the basis of exhaustive swimming time, forelimb grip strength, and levels of serum lactate, ammonia, glucose, blood urea nitrogen (BUN), and creatine kinase (CK) after a swimming exercise.
29973525	7	87	theme	urea	1111:1114	arg1	nitrogen					1116:1123	blood urea nitrogen	1105:1123	blood urea nitrogen (BUN)	1105:1129	In addition, anti-fatigue activity and exercise performance were evaluated on the basis of exhaustive swimming time, forelimb grip strength, and levels of serum lactate, ammonia, glucose, blood urea nitrogen (BUN), and creatine kinase (CK) after a swimming exercise.
29973525	8	88	theme	KF-2X	1355:1359	arg1	strength					1332:1339	the forelimb grip strength	1314:1339	the forelimb grip strength of the KF-1X, KF-2X, and KF-5X groups	1314:1377	The exhaustive swimming time for the KF-1X, KF-2X, and KF-5X groups was significantly longer than that for the vehicle group, and the forelimb grip strength of the KF-1X, KF-2X, and KF-5X groups was also significantly higher than that of the vehicle group.
29973525	8	88	theme	KF-2X	1355:1359	arg1	higher					1402:1407	higher	1402:1407	higher	1402:1407	The exhaustive swimming time for the KF-1X, KF-2X, and KF-5X groups was significantly longer than that for the vehicle group, and the forelimb grip strength of the KF-1X, KF-2X, and KF-5X groups was also significantly higher than that of the vehicle group.
29973525	3	89	theme	=	438:438	arg1	n					436:436	n = 8	436:440	n = 8 per group	436:450	Male ICR mice from four groups (n = 8 per group) were orally administered KF once daily for four weeks at 0, 2.15, 4.31, and 10.76 g/kg/day and were designated as the vehicle, KF-1X, KF-2X, and KF-5X groups, respectively.
29973525	2	90	from	effect	356:361	arg1	composition					391:401	the gut microbiota composition	372:401	the gut microbiota composition	372:401	Furthermore, the composition of the gut microbiota is related to health benefits in the host; therefore, the study also investigated the effect of KF on the gut microbiota composition.
29973525	8	91	theme	groups	1372:1377	arg1	strength					1332:1339	the forelimb grip strength	1314:1339	the forelimb grip strength of the KF-1X, KF-2X, and KF-5X groups	1314:1377	The exhaustive swimming time for the KF-1X, KF-2X, and KF-5X groups was significantly longer than that for the vehicle group, and the forelimb grip strength of the KF-1X, KF-2X, and KF-5X groups was also significantly higher than that of the vehicle group.
29973525	8	91	theme	groups	1372:1377	arg1	higher					1402:1407	higher	1402:1407	higher	1402:1407	The exhaustive swimming time for the KF-1X, KF-2X, and KF-5X groups was significantly longer than that for the vehicle group, and the forelimb grip strength of the KF-1X, KF-2X, and KF-5X groups was also significantly higher than that of the vehicle group.
29973525	4	92	theme	16S	664:666	arg1	sequencing					678:687	16S rRNA gene sequencing	664:687	16S rRNA gene sequencing	664:687	The gut microbiota was analyzed using 16S rRNA gene sequencing.
30551447	1	0	theme	human	371:375	arg1	h-DPSCs					401:407	h-DPSCs	401:407	h-DPSCs	401:407	The purpose of this study was to investigate the effects of Fibrin Glue (FG) and activated platelet-rich plasma (a-PRP) on the proliferation and osteogenic differentiation of human dental pulp stem cells (h-DPSCs).
30551447	1	0	theme	human	371:375	arg1	cells					394:398	human dental pulp stem cells	371:398	human dental pulp stem cells (h-DPSCs)	371:408	The purpose of this study was to investigate the effects of Fibrin Glue (FG) and activated platelet-rich plasma (a-PRP) on the proliferation and osteogenic differentiation of human dental pulp stem cells (h-DPSCs).
30551447	0	1	theme	pulp	179:182	arg1	cells					189:193	seeded human dental pulp stem cells	159:193	seeded human dental pulp stem cells	159:193	Nano-hydroxy apatite/chitosan/gelatin scaffolds enriched by a combination of platelet-rich plasma and fibrin glue enhance proliferation and differentiation of seeded human dental pulp stem cells.
30551447	10	2	theme	elevated	1342:1349	arg1	expression					1501:1510	significantly elevated bone gamma-carboxyglutamic acid-containing protein (BGLAP), bone morphogenetic protein 2 (BMP2), and runt-related transcription factor 2 (RUNX2) gene expression	1328:1510	significantly elevated bone gamma-carboxyglutamic acid-containing protein (BGLAP), bone morphogenetic protein 2 (BMP2), and runt-related transcription factor 2 (RUNX2) gene expression	1328:1510	In addition, the a-PRP-FG treated scaffolds exhibited significantly elevated bone gamma-carboxyglutamic acid-containing protein (BGLAP), bone morphogenetic protein 2 (BMP2), and runt-related transcription factor 2 (RUNX2) gene expression.
30551447	1	3	theme	Glue	263:266	arg1	effects					245:251	the effects	241:251	the effects of Fibrin Glue (FG) and activated platelet-rich plasma (a-PRP) on the proliferation and osteogenic differentiation of human dental pulp stem cells (h-DPSCs)	241:408	The purpose of this study was to investigate the effects of Fibrin Glue (FG) and activated platelet-rich plasma (a-PRP) on the proliferation and osteogenic differentiation of human dental pulp stem cells (h-DPSCs).
30551447	7	4	theme	improved	1050:1057	arg1	adhesion					1059:1066	improved adhesion	1050:1066	improved adhesion of seeded h-DPSCs compared to CS-G/nHA	1050:1105	All groups treated with FG and a-PRP, showed improved adhesion of seeded h-DPSCs compared to CS-G/nHA.
30551447	11	5	dep	result	1525:1530	arg1	scaffolds					1546:1554	the composite scaffolds	1532:1554	The present result the composite scaffolds treated with FG and a-PRP	1513:1580	The present result the composite scaffolds treated with FG and a-PRP showed a fibrin network, preferentially on the surface of composite scaffold increasing the mineralization and osteoblastic differentiation of harvested cells.
30551447	10	6	theme	gene	1496:1499	arg1	expression					1501:1510	significantly elevated bone gamma-carboxyglutamic acid-containing protein (BGLAP), bone morphogenetic protein 2 (BMP2), and runt-related transcription factor 2 (RUNX2) gene expression	1328:1510	significantly elevated bone gamma-carboxyglutamic acid-containing protein (BGLAP), bone morphogenetic protein 2 (BMP2), and runt-related transcription factor 2 (RUNX2) gene expression	1328:1510	In addition, the a-PRP-FG treated scaffolds exhibited significantly elevated bone gamma-carboxyglutamic acid-containing protein (BGLAP), bone morphogenetic protein 2 (BMP2), and runt-related transcription factor 2 (RUNX2) gene expression.
30551447	11	7	theme	fibrin	1591:1596	arg1	network					1598:1604	a fibrin network	1589:1604	a fibrin network	1589:1604	The present result the composite scaffolds treated with FG and a-PRP showed a fibrin network, preferentially on the surface of composite scaffold increasing the mineralization and osteoblastic differentiation of harvested cells.
30551447	10	8	theme	protein	1394:1400	arg1	expression					1501:1510	significantly elevated bone gamma-carboxyglutamic acid-containing protein (BGLAP), bone morphogenetic protein 2 (BMP2), and runt-related transcription factor 2 (RUNX2) gene expression	1328:1510	significantly elevated bone gamma-carboxyglutamic acid-containing protein (BGLAP), bone morphogenetic protein 2 (BMP2), and runt-related transcription factor 2 (RUNX2) gene expression	1328:1510	In addition, the a-PRP-FG treated scaffolds exhibited significantly elevated bone gamma-carboxyglutamic acid-containing protein (BGLAP), bone morphogenetic protein 2 (BMP2), and runt-related transcription factor 2 (RUNX2) gene expression.
30551447	8	9	theme	composite	1128:1136	arg1	scaffolds					1138:1146	the composite scaffolds	1124:1146	the composite scaffolds	1124:1146	Cytotoxicity of the composite scaffolds was assessed by MTT.
30551447	10	10	theme	gamma-carboxyglutamic	1356:1376	arg1	BGLAP					1403:1407	BGLAP	1403:1407	BGLAP	1403:1407	In addition, the a-PRP-FG treated scaffolds exhibited significantly elevated bone gamma-carboxyglutamic acid-containing protein (BGLAP), bone morphogenetic protein 2 (BMP2), and runt-related transcription factor 2 (RUNX2) gene expression.
30551447	10	10	theme	gamma-carboxyglutamic	1356:1376	arg1	protein					1394:1400	bone gamma-carboxyglutamic acid-containing protein	1351:1400	bone gamma-carboxyglutamic acid-containing protein (BGLAP)	1351:1408	In addition, the a-PRP-FG treated scaffolds exhibited significantly elevated bone gamma-carboxyglutamic acid-containing protein (BGLAP), bone morphogenetic protein 2 (BMP2), and runt-related transcription factor 2 (RUNX2) gene expression.
30551447	1	11	theme	dental	377:382	arg1	h-DPSCs					401:407	h-DPSCs	401:407	h-DPSCs	401:407	The purpose of this study was to investigate the effects of Fibrin Glue (FG) and activated platelet-rich plasma (a-PRP) on the proliferation and osteogenic differentiation of human dental pulp stem cells (h-DPSCs).
30551447	1	11	theme	dental	377:382	arg1	cells					394:398	human dental pulp stem cells	371:398	human dental pulp stem cells (h-DPSCs)	371:408	The purpose of this study was to investigate the effects of Fibrin Glue (FG) and activated platelet-rich plasma (a-PRP) on the proliferation and osteogenic differentiation of human dental pulp stem cells (h-DPSCs).
30551447	1	12	theme	study	216:220	arg1	purpose					200:206	The purpose	196:206	The purpose of this study	196:220	The purpose of this study was to investigate the effects of Fibrin Glue (FG) and activated platelet-rich plasma (a-PRP) on the proliferation and osteogenic differentiation of human dental pulp stem cells (h-DPSCs).
30551447	0	13	theme	seeded	159:164	arg1	cells					189:193	seeded human dental pulp stem cells	159:193	seeded human dental pulp stem cells	159:193	Nano-hydroxy apatite/chitosan/gelatin scaffolds enriched by a combination of platelet-rich plasma and fibrin glue enhance proliferation and differentiation of seeded human dental pulp stem cells.
30551447	1	14	theme	stem	389:392	arg1	h-DPSCs					401:407	h-DPSCs	401:407	h-DPSCs	401:407	The purpose of this study was to investigate the effects of Fibrin Glue (FG) and activated platelet-rich plasma (a-PRP) on the proliferation and osteogenic differentiation of human dental pulp stem cells (h-DPSCs).
30551447	1	14	theme	stem	389:392	arg1	cells					394:398	human dental pulp stem cells	371:398	human dental pulp stem cells (h-DPSCs)	371:408	The purpose of this study was to investigate the effects of Fibrin Glue (FG) and activated platelet-rich plasma (a-PRP) on the proliferation and osteogenic differentiation of human dental pulp stem cells (h-DPSCs).
30551447	0	15	theme	dental	172:177	arg1	cells					189:193	seeded human dental pulp stem cells	159:193	seeded human dental pulp stem cells	159:193	Nano-hydroxy apatite/chitosan/gelatin scaffolds enriched by a combination of platelet-rich plasma and fibrin glue enhance proliferation and differentiation of seeded human dental pulp stem cells.
30551447	11	16	theme	osteoblastic	1693:1704	arg1	differentiation					1706:1720	osteoblastic differentiation	1693:1720	osteoblastic differentiation	1693:1720	The present result the composite scaffolds treated with FG and a-PRP showed a fibrin network, preferentially on the surface of composite scaffold increasing the mineralization and osteoblastic differentiation of harvested cells.
30551447	0	17	theme	stem	184:187	arg1	cells					189:193	seeded human dental pulp stem cells	159:193	seeded human dental pulp stem cells	159:193	Nano-hydroxy apatite/chitosan/gelatin scaffolds enriched by a combination of platelet-rich plasma and fibrin glue enhance proliferation and differentiation of seeded human dental pulp stem cells.
30551447	6	18	theme	fibrin	968:973	arg1	network					975:981	organized fibrin network	958:981	organized fibrin network	958:981	The 14 days SEM image reveled organized fibrin network on scaffolds surface.
30551447	2	19	theme	composite	476:484	arg1	scaffolds					486:494	porous composite scaffolds	469:494	porous composite scaffolds based on chitosan-gelatin/nanohydroxyapatite (CS-G/nHA) treated with FG and a-PRP	469:576	Therefore, we planned to investigate in vitro behavior of porous composite scaffolds based on chitosan-gelatin/nanohydroxyapatite (CS-G/nHA) treated with FG and a-PRP.
30551447	0	20	theme	platelet-rich	77:89	arg1	plasma					91:96	platelet-rich plasma	77:96	platelet-rich plasma	77:96	Nano-hydroxy apatite/chitosan/gelatin scaffolds enriched by a combination of platelet-rich plasma and fibrin glue enhance proliferation and differentiation of seeded human dental pulp stem cells.
30551447	9	21	theme	minerals	1223:1230	arg1	formation					1205:1213	the formation	1201:1213	the formation of bone minerals by h-DPSCs	1201:1241	Alizarin red staining confirmed the formation of bone minerals by h-DPSCs after 21 days of cell seeding.
30551447	3	22	theme	CS-G/nHA	603:610	arg1	structure					590:598	The porous structure	579:598	The porous structure of CS-G/nHA	579:610	The porous structure of CS-G/nHA was prepared using combination of particle leaching and freeze-drying methods.
30551447	5	23	dep	a-PRP/CS-G/nHA	898:911	arg1	3					895:895	3	895:895	3	895:895	Four groups of composite scaffolds were fabricated to seed h-DPSCs: (1) a-PRP-FG/CS-G/nHA; (2) FG/CS-G/nHA; (3) a-PRP/CS-G/nHA; (4) CS-G/nHA.
30551447	11	24	theme	cells	1735:1739	arg1	mineralization					1674:1687	mineralization	1674:1687	mineralization	1674:1687	The present result the composite scaffolds treated with FG and a-PRP showed a fibrin network, preferentially on the surface of composite scaffold increasing the mineralization and osteoblastic differentiation of harvested cells.
30551447	11	24	theme	cells	1735:1739	arg1	differentiation					1706:1720	osteoblastic differentiation	1693:1720	osteoblastic differentiation	1693:1720	The present result the composite scaffolds treated with FG and a-PRP showed a fibrin network, preferentially on the surface of composite scaffold increasing the mineralization and osteoblastic differentiation of harvested cells.
30551447	6	25	theme	SEM	940:942	arg1	image					944:948	The 14 days SEM image	928:948	The 14 days SEM image	928:948	The 14 days SEM image reveled organized fibrin network on scaffolds surface.
30551447	4	26	theme	calcium	768:774	arg1	chloride					776:783	calcium chloride	768:783	calcium chloride	768:783	The a-PRP was prepared from the centrifugation of whole blood activated with calcium chloride.
30551447	0	27	theme	glue	109:112	arg1	combination					62:72	a combination	60:72	a combination of platelet-rich plasma and fibrin glue	60:112	Nano-hydroxy apatite/chitosan/gelatin scaffolds enriched by a combination of platelet-rich plasma and fibrin glue enhance proliferation and differentiation of seeded human dental pulp stem cells.
30551447	1	28	theme	osteogenic	341:350	arg1	differentiation					352:366	osteogenic differentiation	341:366	osteogenic differentiation	341:366	The purpose of this study was to investigate the effects of Fibrin Glue (FG) and activated platelet-rich plasma (a-PRP) on the proliferation and osteogenic differentiation of human dental pulp stem cells (h-DPSCs).
30551447	2	29	dep	in	448:449	arg1	vitro					451:455	vitro	451:455	vitro	451:455	Therefore, we planned to investigate in vitro behavior of porous composite scaffolds based on chitosan-gelatin/nanohydroxyapatite (CS-G/nHA) treated with FG and a-PRP.
30551447	10	30	theme	morphogenetic	1416:1428	arg1	protein					1430:1436	bone morphogenetic protein 2	1411:1438	bone morphogenetic protein 2 (BMP2)	1411:1445	In addition, the a-PRP-FG treated scaffolds exhibited significantly elevated bone gamma-carboxyglutamic acid-containing protein (BGLAP), bone morphogenetic protein 2 (BMP2), and runt-related transcription factor 2 (RUNX2) gene expression.
30551447	10	30	theme	morphogenetic	1416:1428	arg1	BMP2					1441:1444	BMP2	1441:1444	BMP2	1441:1444	In addition, the a-PRP-FG treated scaffolds exhibited significantly elevated bone gamma-carboxyglutamic acid-containing protein (BGLAP), bone morphogenetic protein 2 (BMP2), and runt-related transcription factor 2 (RUNX2) gene expression.
30551447	4	31	theme	whole	741:745	arg1	blood					747:751	whole blood	741:751	whole blood activated with calcium chloride	741:783	The a-PRP was prepared from the centrifugation of whole blood activated with calcium chloride.
30551447	0	32	theme	Nano-hydroxy	0:11	arg1	scaffolds					38:46	Nano-hydroxy apatite/chitosan/gelatin scaffolds	0:46	Nano-hydroxy apatite/chitosan/gelatin scaffolds enriched by a combination of platelet-rich plasma and fibrin glue	0:112	Nano-hydroxy apatite/chitosan/gelatin scaffolds enriched by a combination of platelet-rich plasma and fibrin glue enhance proliferation and differentiation of seeded human dental pulp stem cells.
30551447	9	33	theme	seeding	1265:1271	arg1	days					1252:1255	21 days	1249:1255	21 days of cell seeding	1249:1271	Alizarin red staining confirmed the formation of bone minerals by h-DPSCs after 21 days of cell seeding.
30551447	1	34	theme	activated	277:285	arg1	a-PRP					309:313	a-PRP	309:313	a-PRP	309:313	The purpose of this study was to investigate the effects of Fibrin Glue (FG) and activated platelet-rich plasma (a-PRP) on the proliferation and osteogenic differentiation of human dental pulp stem cells (h-DPSCs).
30551447	1	34	theme	activated	277:285	arg1	plasma					301:306	activated platelet-rich plasma	277:306	activated platelet-rich plasma (a-PRP)	277:314	The purpose of this study was to investigate the effects of Fibrin Glue (FG) and activated platelet-rich plasma (a-PRP) on the proliferation and osteogenic differentiation of human dental pulp stem cells (h-DPSCs).
30551447	3	35	theme	particle	646:653	arg1	leaching					655:662	particle leaching	646:662	particle leaching	646:662	The porous structure of CS-G/nHA was prepared using combination of particle leaching and freeze-drying methods.
30551447	5	36	dep	a-PRP-FG/CS-G/nHA	858:874	arg1	1					855:855	1	855:855	1	855:855	Four groups of composite scaffolds were fabricated to seed h-DPSCs: (1) a-PRP-FG/CS-G/nHA; (2) FG/CS-G/nHA; (3) a-PRP/CS-G/nHA; (4) CS-G/nHA.
30551447	1	37	theme	plasma	301:306	arg1	effects					245:251	the effects	241:251	the effects of Fibrin Glue (FG) and activated platelet-rich plasma (a-PRP) on the proliferation and osteogenic differentiation of human dental pulp stem cells (h-DPSCs)	241:408	The purpose of this study was to investigate the effects of Fibrin Glue (FG) and activated platelet-rich plasma (a-PRP) on the proliferation and osteogenic differentiation of human dental pulp stem cells (h-DPSCs).
30551447	10	38	theme	factor	1479:1484	arg1	expression					1501:1510	significantly elevated bone gamma-carboxyglutamic acid-containing protein (BGLAP), bone morphogenetic protein 2 (BMP2), and runt-related transcription factor 2 (RUNX2) gene expression	1328:1510	significantly elevated bone gamma-carboxyglutamic acid-containing protein (BGLAP), bone morphogenetic protein 2 (BMP2), and runt-related transcription factor 2 (RUNX2) gene expression	1328:1510	In addition, the a-PRP-FG treated scaffolds exhibited significantly elevated bone gamma-carboxyglutamic acid-containing protein (BGLAP), bone morphogenetic protein 2 (BMP2), and runt-related transcription factor 2 (RUNX2) gene expression.
30551447	11	39	theme	composite	1536:1544	arg1	scaffolds					1546:1554	the composite scaffolds	1532:1554	The present result the composite scaffolds treated with FG and a-PRP	1513:1580	The present result the composite scaffolds treated with FG and a-PRP showed a fibrin network, preferentially on the surface of composite scaffold increasing the mineralization and osteoblastic differentiation of harvested cells.
30551447	5	40	theme	composite	801:809	arg1	scaffolds					811:819	composite scaffolds	801:819	composite scaffolds	801:819	Four groups of composite scaffolds were fabricated to seed h-DPSCs: (1) a-PRP-FG/CS-G/nHA; (2) FG/CS-G/nHA; (3) a-PRP/CS-G/nHA; (4) CS-G/nHA.
30551447	12	41	theme	marker	1798:1803	arg1	expressions					1810:1820	bone marker gene expressions	1793:1820	bone marker gene expressions	1793:1820	In addition, a-PRP-FG/ CS-G/nHA scaffold increased bone marker gene expressions from day 7 to day 21.
30551447	12	42	theme	bone	1793:1796	arg1	expressions					1810:1820	bone marker gene expressions	1793:1820	bone marker gene expressions	1793:1820	In addition, a-PRP-FG/ CS-G/nHA scaffold increased bone marker gene expressions from day 7 to day 21.
30551447	10	43	theme	runt-related	1452:1463	arg1	RUNX2					1489:1493	RUNX2	1489:1493	RUNX2	1489:1493	In addition, the a-PRP-FG treated scaffolds exhibited significantly elevated bone gamma-carboxyglutamic acid-containing protein (BGLAP), bone morphogenetic protein 2 (BMP2), and runt-related transcription factor 2 (RUNX2) gene expression.
30551447	10	43	theme	runt-related	1452:1463	arg1	factor					1479:1484	runt-related transcription factor 2	1452:1486	runt-related transcription factor 2 (RUNX2)	1452:1494	In addition, the a-PRP-FG treated scaffolds exhibited significantly elevated bone gamma-carboxyglutamic acid-containing protein (BGLAP), bone morphogenetic protein 2 (BMP2), and runt-related transcription factor 2 (RUNX2) gene expression.
30551447	3	44	theme	methods	682:688	arg1	combination					631:641	combination	631:641	combination of particle leaching and freeze-drying methods	631:688	The porous structure of CS-G/nHA was prepared using combination of particle leaching and freeze-drying methods.
30551447	11	45	theme	scaffold	1650:1657	arg1	surface					1629:1635	the surface	1625:1635	the surface of composite scaffold increasing the mineralization and osteoblastic differentiation of harvested cells	1625:1739	The present result the composite scaffolds treated with FG and a-PRP showed a fibrin network, preferentially on the surface of composite scaffold increasing the mineralization and osteoblastic differentiation of harvested cells.
30551447	9	46	theme	Alizarin	1169:1176	arg1	staining					1182:1189	Alizarin red staining	1169:1189	Alizarin red staining	1169:1189	Alizarin red staining confirmed the formation of bone minerals by h-DPSCs after 21 days of cell seeding.
30551447	10	47	theme	a-PRP-FG	1291:1298	arg1	scaffolds					1308:1316	the a-PRP-FG treated scaffolds	1287:1316	the a-PRP-FG treated scaffolds	1287:1316	In addition, the a-PRP-FG treated scaffolds exhibited significantly elevated bone gamma-carboxyglutamic acid-containing protein (BGLAP), bone morphogenetic protein 2 (BMP2), and runt-related transcription factor 2 (RUNX2) gene expression.
30551447	12	48	theme	a-PRP-FG/	1755:1763	arg1	scaffold					1774:1781	a-PRP-FG/ CS-G/nHA scaffold	1755:1781	a-PRP-FG/ CS-G/nHA scaffold	1755:1781	In addition, a-PRP-FG/ CS-G/nHA scaffold increased bone marker gene expressions from day 7 to day 21.
30551447	1	49	theme	Fibrin	256:261	arg1	Glue					263:266	Fibrin Glue	256:266	Fibrin Glue (FG)	256:271	The purpose of this study was to investigate the effects of Fibrin Glue (FG) and activated platelet-rich plasma (a-PRP) on the proliferation and osteogenic differentiation of human dental pulp stem cells (h-DPSCs).
30551447	1	49	theme	Fibrin	256:261	arg1	FG					269:270	FG	269:270	FG	269:270	The purpose of this study was to investigate the effects of Fibrin Glue (FG) and activated platelet-rich plasma (a-PRP) on the proliferation and osteogenic differentiation of human dental pulp stem cells (h-DPSCs).
30551447	7	50	theme	h-DPSCs	1078:1084	arg1	adhesion					1059:1066	improved adhesion	1050:1066	improved adhesion of seeded h-DPSCs compared to CS-G/nHA	1050:1105	All groups treated with FG and a-PRP, showed improved adhesion of seeded h-DPSCs compared to CS-G/nHA.
30551447	10	51	theme	acid-containing	1378:1392	arg1	BGLAP					1403:1407	BGLAP	1403:1407	BGLAP	1403:1407	In addition, the a-PRP-FG treated scaffolds exhibited significantly elevated bone gamma-carboxyglutamic acid-containing protein (BGLAP), bone morphogenetic protein 2 (BMP2), and runt-related transcription factor 2 (RUNX2) gene expression.
30551447	10	51	theme	acid-containing	1378:1392	arg1	protein					1394:1400	bone gamma-carboxyglutamic acid-containing protein	1351:1400	bone gamma-carboxyglutamic acid-containing protein (BGLAP)	1351:1408	In addition, the a-PRP-FG treated scaffolds exhibited significantly elevated bone gamma-carboxyglutamic acid-containing protein (BGLAP), bone morphogenetic protein 2 (BMP2), and runt-related transcription factor 2 (RUNX2) gene expression.
30551447	10	52	theme	bone	1351:1354	arg1	BGLAP					1403:1407	BGLAP	1403:1407	BGLAP	1403:1407	In addition, the a-PRP-FG treated scaffolds exhibited significantly elevated bone gamma-carboxyglutamic acid-containing protein (BGLAP), bone morphogenetic protein 2 (BMP2), and runt-related transcription factor 2 (RUNX2) gene expression.
30551447	10	52	theme	bone	1351:1354	arg1	protein					1394:1400	bone gamma-carboxyglutamic acid-containing protein	1351:1400	bone gamma-carboxyglutamic acid-containing protein (BGLAP)	1351:1408	In addition, the a-PRP-FG treated scaffolds exhibited significantly elevated bone gamma-carboxyglutamic acid-containing protein (BGLAP), bone morphogenetic protein 2 (BMP2), and runt-related transcription factor 2 (RUNX2) gene expression.
30551447	8	53	theme	scaffolds	1138:1146	arg1	Cytotoxicity					1108:1119	Cytotoxicity	1108:1119	Cytotoxicity of the composite scaffolds	1108:1146	Cytotoxicity of the composite scaffolds was assessed by MTT.
30551447	0	54	theme	human	166:170	arg1	cells					189:193	seeded human dental pulp stem cells	159:193	seeded human dental pulp stem cells	159:193	Nano-hydroxy apatite/chitosan/gelatin scaffolds enriched by a combination of platelet-rich plasma and fibrin glue enhance proliferation and differentiation of seeded human dental pulp stem cells.
30551447	9	55	theme	red	1178:1180	arg1	staining					1182:1189	Alizarin red staining	1169:1189	Alizarin red staining	1169:1189	Alizarin red staining confirmed the formation of bone minerals by h-DPSCs after 21 days of cell seeding.
30551447	3	56	theme	porous	583:588	arg1	structure					590:598	The porous structure	579:598	The porous structure of CS-G/nHA	579:610	The porous structure of CS-G/nHA was prepared using combination of particle leaching and freeze-drying methods.
30551447	1	57	from	effects	245:251	arg1	proliferation					323:335	proliferation	323:335	proliferation	323:335	The purpose of this study was to investigate the effects of Fibrin Glue (FG) and activated platelet-rich plasma (a-PRP) on the proliferation and osteogenic differentiation of human dental pulp stem cells (h-DPSCs).
30551447	1	57	from	effects	245:251	arg1	differentiation					352:366	osteogenic differentiation	341:366	osteogenic differentiation	341:366	The purpose of this study was to investigate the effects of Fibrin Glue (FG) and activated platelet-rich plasma (a-PRP) on the proliferation and osteogenic differentiation of human dental pulp stem cells (h-DPSCs).
30551447	1	58	theme	cells	394:398	arg1	proliferation					323:335	proliferation	323:335	proliferation	323:335	The purpose of this study was to investigate the effects of Fibrin Glue (FG) and activated platelet-rich plasma (a-PRP) on the proliferation and osteogenic differentiation of human dental pulp stem cells (h-DPSCs).
30551447	1	58	theme	cells	394:398	arg1	differentiation					352:366	osteogenic differentiation	341:366	osteogenic differentiation	341:366	The purpose of this study was to investigate the effects of Fibrin Glue (FG) and activated platelet-rich plasma (a-PRP) on the proliferation and osteogenic differentiation of human dental pulp stem cells (h-DPSCs).
30551447	0	59	theme	cells	189:193	arg1	differentiation					140:154	differentiation	140:154	differentiation	140:154	Nano-hydroxy apatite/chitosan/gelatin scaffolds enriched by a combination of platelet-rich plasma and fibrin glue enhance proliferation and differentiation of seeded human dental pulp stem cells.
30551447	0	59	theme	cells	189:193	arg1	proliferation					122:134	proliferation	122:134	proliferation	122:134	Nano-hydroxy apatite/chitosan/gelatin scaffolds enriched by a combination of platelet-rich plasma and fibrin glue enhance proliferation and differentiation of seeded human dental pulp stem cells.
30551447	5	60	dep	CS-G/nHA	918:925	arg1	4					915:915	4	915:915	4	915:915	Four groups of composite scaffolds were fabricated to seed h-DPSCs: (1) a-PRP-FG/CS-G/nHA; (2) FG/CS-G/nHA; (3) a-PRP/CS-G/nHA; (4) CS-G/nHA.
30551447	0	61	theme	plasma	91:96	arg1	combination					62:72	a combination	60:72	a combination of platelet-rich plasma and fibrin glue	60:112	Nano-hydroxy apatite/chitosan/gelatin scaffolds enriched by a combination of platelet-rich plasma and fibrin glue enhance proliferation and differentiation of seeded human dental pulp stem cells.
30551447	9	62	theme	bone	1218:1221	arg1	minerals					1223:1230	bone minerals	1218:1230	bone minerals	1218:1230	Alizarin red staining confirmed the formation of bone minerals by h-DPSCs after 21 days of cell seeding.
30551447	6	63	theme	organized	958:966	arg1	network					975:981	organized fibrin network	958:981	organized fibrin network	958:981	The 14 days SEM image reveled organized fibrin network on scaffolds surface.
30551447	2	64	theme	porous	469:474	arg1	scaffolds					486:494	porous composite scaffolds	469:494	porous composite scaffolds based on chitosan-gelatin/nanohydroxyapatite (CS-G/nHA) treated with FG and a-PRP	469:576	Therefore, we planned to investigate in vitro behavior of porous composite scaffolds based on chitosan-gelatin/nanohydroxyapatite (CS-G/nHA) treated with FG and a-PRP.
30551447	0	65	theme	fibrin	102:107	arg1	glue					109:112	fibrin glue	102:112	fibrin glue	102:112	Nano-hydroxy apatite/chitosan/gelatin scaffolds enriched by a combination of platelet-rich plasma and fibrin glue enhance proliferation and differentiation of seeded human dental pulp stem cells.
30551447	3	66	theme	leaching	655:662	arg1	combination					631:641	combination	631:641	combination of particle leaching and freeze-drying methods	631:688	The porous structure of CS-G/nHA was prepared using combination of particle leaching and freeze-drying methods.
30551447	11	67	dep	mineralization	1674:1687	arg1	the					1670:1672	the	1670:1672	the	1670:1672	The present result the composite scaffolds treated with FG and a-PRP showed a fibrin network, preferentially on the surface of composite scaffold increasing the mineralization and osteoblastic differentiation of harvested cells.
30551447	6	68	theme	days	935:938	arg1	image					944:948	The 14 days SEM image	928:948	The 14 days SEM image	928:948	The 14 days SEM image reveled organized fibrin network on scaffolds surface.
30551447	11	69	theme	harvested	1725:1733	arg1	cells					1735:1739	harvested cells	1725:1739	harvested cells	1725:1739	The present result the composite scaffolds treated with FG and a-PRP showed a fibrin network, preferentially on the surface of composite scaffold increasing the mineralization and osteoblastic differentiation of harvested cells.
30551447	2	70	theme	scaffolds	486:494	arg1	behavior					457:464	in vitro behavior	448:464	in vitro behavior of porous composite scaffolds based on chitosan-gelatin/nanohydroxyapatite (CS-G/nHA) treated with FG and a-PRP	448:576	Therefore, we planned to investigate in vitro behavior of porous composite scaffolds based on chitosan-gelatin/nanohydroxyapatite (CS-G/nHA) treated with FG and a-PRP.
30551447	10	71	theme	protein	1430:1436	arg1	expression					1501:1510	significantly elevated bone gamma-carboxyglutamic acid-containing protein (BGLAP), bone morphogenetic protein 2 (BMP2), and runt-related transcription factor 2 (RUNX2) gene expression	1328:1510	significantly elevated bone gamma-carboxyglutamic acid-containing protein (BGLAP), bone morphogenetic protein 2 (BMP2), and runt-related transcription factor 2 (RUNX2) gene expression	1328:1510	In addition, the a-PRP-FG treated scaffolds exhibited significantly elevated bone gamma-carboxyglutamic acid-containing protein (BGLAP), bone morphogenetic protein 2 (BMP2), and runt-related transcription factor 2 (RUNX2) gene expression.
30551447	0	72	theme	apatite/chitosan/gelatin	13:36	arg1	scaffolds					38:46	Nano-hydroxy apatite/chitosan/gelatin scaffolds	0:46	Nano-hydroxy apatite/chitosan/gelatin scaffolds enriched by a combination of platelet-rich plasma and fibrin glue	0:112	Nano-hydroxy apatite/chitosan/gelatin scaffolds enriched by a combination of platelet-rich plasma and fibrin glue enhance proliferation and differentiation of seeded human dental pulp stem cells.
30551447	9	73	theme	cell	1260:1263	arg1	seeding					1265:1271	cell seeding	1260:1271	cell seeding	1260:1271	Alizarin red staining confirmed the formation of bone minerals by h-DPSCs after 21 days of cell seeding.
30551447	1	74	dep	proliferation	323:335	arg1	the					319:321	the	319:321	the	319:321	The purpose of this study was to investigate the effects of Fibrin Glue (FG) and activated platelet-rich plasma (a-PRP) on the proliferation and osteogenic differentiation of human dental pulp stem cells (h-DPSCs).
30551447	10	75	theme	bone	1411:1414	arg1	protein					1430:1436	bone morphogenetic protein 2	1411:1438	bone morphogenetic protein 2 (BMP2)	1411:1445	In addition, the a-PRP-FG treated scaffolds exhibited significantly elevated bone gamma-carboxyglutamic acid-containing protein (BGLAP), bone morphogenetic protein 2 (BMP2), and runt-related transcription factor 2 (RUNX2) gene expression.
30551447	10	75	theme	bone	1411:1414	arg1	BMP2					1441:1444	BMP2	1441:1444	BMP2	1441:1444	In addition, the a-PRP-FG treated scaffolds exhibited significantly elevated bone gamma-carboxyglutamic acid-containing protein (BGLAP), bone morphogenetic protein 2 (BMP2), and runt-related transcription factor 2 (RUNX2) gene expression.
30551447	5	76	dep	FG/CS-G/nHA	881:891	arg1	2					878:878	2	878:878	2	878:878	Four groups of composite scaffolds were fabricated to seed h-DPSCs: (1) a-PRP-FG/CS-G/nHA; (2) FG/CS-G/nHA; (3) a-PRP/CS-G/nHA; (4) CS-G/nHA.
30551447	1	77	theme	pulp	384:387	arg1	h-DPSCs					401:407	h-DPSCs	401:407	h-DPSCs	401:407	The purpose of this study was to investigate the effects of Fibrin Glue (FG) and activated platelet-rich plasma (a-PRP) on the proliferation and osteogenic differentiation of human dental pulp stem cells (h-DPSCs).
30551447	1	77	theme	pulp	384:387	arg1	cells					394:398	human dental pulp stem cells	371:398	human dental pulp stem cells (h-DPSCs)	371:408	The purpose of this study was to investigate the effects of Fibrin Glue (FG) and activated platelet-rich plasma (a-PRP) on the proliferation and osteogenic differentiation of human dental pulp stem cells (h-DPSCs).
30551447	4	78	theme	blood	747:751	arg1	centrifugation					723:736	the centrifugation	719:736	the centrifugation of whole blood activated with calcium chloride	719:783	The a-PRP was prepared from the centrifugation of whole blood activated with calcium chloride.
30551447	1	79	theme	platelet-rich	287:299	arg1	a-PRP					309:313	a-PRP	309:313	a-PRP	309:313	The purpose of this study was to investigate the effects of Fibrin Glue (FG) and activated platelet-rich plasma (a-PRP) on the proliferation and osteogenic differentiation of human dental pulp stem cells (h-DPSCs).
30551447	1	79	theme	platelet-rich	287:299	arg1	plasma					301:306	activated platelet-rich plasma	277:306	activated platelet-rich plasma (a-PRP)	277:314	The purpose of this study was to investigate the effects of Fibrin Glue (FG) and activated platelet-rich plasma (a-PRP) on the proliferation and osteogenic differentiation of human dental pulp stem cells (h-DPSCs).
30551447	6	80	theme	scaffolds	986:994	arg1	surface					996:1002	scaffolds surface	986:1002	scaffolds surface	986:1002	The 14 days SEM image reveled organized fibrin network on scaffolds surface.
30551447	2	81	theme	in	448:449	arg1	behavior					457:464	in vitro behavior	448:464	in vitro behavior of porous composite scaffolds based on chitosan-gelatin/nanohydroxyapatite (CS-G/nHA) treated with FG and a-PRP	448:576	Therefore, we planned to investigate in vitro behavior of porous composite scaffolds based on chitosan-gelatin/nanohydroxyapatite (CS-G/nHA) treated with FG and a-PRP.
30551447	7	82	theme	seeded	1071:1076	arg1	h-DPSCs					1078:1084	seeded h-DPSCs	1071:1084	seeded h-DPSCs	1071:1084	All groups treated with FG and a-PRP, showed improved adhesion of seeded h-DPSCs compared to CS-G/nHA.
30551447	12	83	theme	gene	1805:1808	arg1	expressions					1810:1820	bone marker gene expressions	1793:1820	bone marker gene expressions	1793:1820	In addition, a-PRP-FG/ CS-G/nHA scaffold increased bone marker gene expressions from day 7 to day 21.
30551447	12	84	theme	CS-G/nHA	1765:1772	arg1	scaffold					1774:1781	a-PRP-FG/ CS-G/nHA scaffold	1755:1781	a-PRP-FG/ CS-G/nHA scaffold	1755:1781	In addition, a-PRP-FG/ CS-G/nHA scaffold increased bone marker gene expressions from day 7 to day 21.
30551447	10	85	theme	transcription	1465:1477	arg1	RUNX2					1489:1493	RUNX2	1489:1493	RUNX2	1489:1493	In addition, the a-PRP-FG treated scaffolds exhibited significantly elevated bone gamma-carboxyglutamic acid-containing protein (BGLAP), bone morphogenetic protein 2 (BMP2), and runt-related transcription factor 2 (RUNX2) gene expression.
30551447	10	85	theme	transcription	1465:1477	arg1	factor					1479:1484	runt-related transcription factor 2	1452:1486	runt-related transcription factor 2 (RUNX2)	1452:1494	In addition, the a-PRP-FG treated scaffolds exhibited significantly elevated bone gamma-carboxyglutamic acid-containing protein (BGLAP), bone morphogenetic protein 2 (BMP2), and runt-related transcription factor 2 (RUNX2) gene expression.
30551447	3	86	theme	freeze-drying	668:680	arg1	methods					682:688	freeze-drying methods	668:688	freeze-drying methods	668:688	The porous structure of CS-G/nHA was prepared using combination of particle leaching and freeze-drying methods.
30551447	11	87	theme	present	1517:1523	arg1	result					1525:1530	The present result the composite scaffolds treated with FG and a-PRP	1513:1580	The present result the composite scaffolds treated with FG and a-PRP	1513:1580	The present result the composite scaffolds treated with FG and a-PRP showed a fibrin network, preferentially on the surface of composite scaffold increasing the mineralization and osteoblastic differentiation of harvested cells.
30551447	11	88	theme	composite	1640:1648	arg1	scaffold					1650:1657	composite scaffold	1640:1657	composite scaffold increasing the mineralization and osteoblastic differentiation of harvested cells	1640:1739	The present result the composite scaffolds treated with FG and a-PRP showed a fibrin network, preferentially on the surface of composite scaffold increasing the mineralization and osteoblastic differentiation of harvested cells.
30551447	10	89	theme	treated	1300:1306	arg1	scaffolds					1308:1316	the a-PRP-FG treated scaffolds	1287:1316	the a-PRP-FG treated scaffolds	1287:1316	In addition, the a-PRP-FG treated scaffolds exhibited significantly elevated bone gamma-carboxyglutamic acid-containing protein (BGLAP), bone morphogenetic protein 2 (BMP2), and runt-related transcription factor 2 (RUNX2) gene expression.
30551447	5	90	dep	h-DPSCs	845:851	arg1	CS-G/nHA					918:925	(4) CS-G/nHA	914:925	h-DPSCs: (1) a-PRP-FG/CS-G/nHA; (2) FG/CS-G/nHA; (3) a-PRP/CS-G/nHA; (4) CS-G/nHA	845:925	Four groups of composite scaffolds were fabricated to seed h-DPSCs: (1) a-PRP-FG/CS-G/nHA; (2) FG/CS-G/nHA; (3) a-PRP/CS-G/nHA; (4) CS-G/nHA.
30551447	5	90	dep	h-DPSCs	845:851	arg1	a-PRP/CS-G/nHA					898:911	(3) a-PRP/CS-G/nHA	894:911	h-DPSCs: (1) a-PRP-FG/CS-G/nHA; (2) FG/CS-G/nHA; (3) a-PRP/CS-G/nHA; (4) CS-G/nHA	845:925	Four groups of composite scaffolds were fabricated to seed h-DPSCs: (1) a-PRP-FG/CS-G/nHA; (2) FG/CS-G/nHA; (3) a-PRP/CS-G/nHA; (4) CS-G/nHA.
30551447	5	90	dep	h-DPSCs	845:851	arg1	FG/CS-G/nHA					881:891	(2) FG/CS-G/nHA	877:891	h-DPSCs: (1) a-PRP-FG/CS-G/nHA; (2) FG/CS-G/nHA; (3) a-PRP/CS-G/nHA; (4) CS-G/nHA	845:925	Four groups of composite scaffolds were fabricated to seed h-DPSCs: (1) a-PRP-FG/CS-G/nHA; (2) FG/CS-G/nHA; (3) a-PRP/CS-G/nHA; (4) CS-G/nHA.
30551447	5	90	dep	h-DPSCs	845:851	arg1	a-PRP-FG/CS-G/nHA					858:874	(1) a-PRP-FG/CS-G/nHA	854:874	h-DPSCs: (1) a-PRP-FG/CS-G/nHA; (2) FG/CS-G/nHA; (3) a-PRP/CS-G/nHA; (4) CS-G/nHA	845:925	Four groups of composite scaffolds were fabricated to seed h-DPSCs: (1) a-PRP-FG/CS-G/nHA; (2) FG/CS-G/nHA; (3) a-PRP/CS-G/nHA; (4) CS-G/nHA.
30551447	5	91	theme	scaffolds	811:819	arg1	groups					791:796	Four groups	786:796	Four groups of composite scaffolds	786:819	Four groups of composite scaffolds were fabricated to seed h-DPSCs: (1) a-PRP-FG/CS-G/nHA; (2) FG/CS-G/nHA; (3) a-PRP/CS-G/nHA; (4) CS-G/nHA.
30551447	5	91	theme	scaffolds	811:819	arg1	scaffolds					811:819	composite scaffolds	801:819	composite scaffolds	801:819	Four groups of composite scaffolds were fabricated to seed h-DPSCs: (1) a-PRP-FG/CS-G/nHA; (2) FG/CS-G/nHA; (3) a-PRP/CS-G/nHA; (4) CS-G/nHA.
31234423	12	0	theme	cell	1826:1829	arg1	wall					1831:1834	the cell wall	1822:1834	the cell wall of barley root cells under stress conditions	1822:1879	The presented data extend upon the knowledge on the chemical composition of the cell wall of barley root cells under stress conditions.
31234423	9	1	theme	arising	1468:1474	arg1	walls					1481:1485	newly arising cell walls	1462:1485	newly arising cell walls	1462:1485	Based on the presented results, it was concluded that methyl-esterified HG can be a marker for newly arising cell walls.
31234423	7	2	theme	fluorescence	1060:1071	arg1	intensity					1073:1081	a visually lower fluorescence intensity	1043:1081	a visually lower fluorescence intensity than in the control roots	1043:1107	In the Al-treated roots, pectins with galactan sidechains were detected with a visually lower fluorescence intensity than in the control roots while pectins with arabinan sidechains were abundantly present.
31234423	13	3	from	knowledge	2007:2015	arg1	toxicity					2023:2030	Al toxicity	2020:2030	Al toxicity	2020:2030	The response of cells to Al can be expressed by the specific distribution of pectins in the cell wall and, thus, enables the knowledge on Al toxicity to be extended by explaining the mechanism by which Al inhibits root elongation.
31234423	3	4	theme	specific	399:406	arg1	epitopes					415:422	specific pectic epitopes	399:422	specific pectic epitopes	399:422	In this study, we evaluate the possible involvement of specific pectic epitopes in the cells of barley roots in response to aluminum exposure.
31234423	7	5	from	intensity	1073:1081	arg1	roots					1103:1107	the control roots	1091:1107	the control roots	1091:1107	In the Al-treated roots, pectins with galactan sidechains were detected with a visually lower fluorescence intensity than in the control roots while pectins with arabinan sidechains were abundantly present.
31234423	8	6	attach	present	1338:1344	arg2	HGs					1329:1331	methyl-esterified HGs	1311:1331	methyl-esterified HGs	1311:1331	Furthermore, esterified homogalacturonans (HGs) were present with a visually higher fluorescence intensity compared to the control, while methyl-esterified HGs were present in a similar amount.
31234423	8	6	attach	present	1338:1344	arg1	amount					1359:1364	a similar amount	1349:1364	a similar amount	1349:1364	Furthermore, esterified homogalacturonans (HGs) were present with a visually higher fluorescence intensity compared to the control, while methyl-esterified HGs were present in a similar amount.
31234423	12	7	theme	chemical	1798:1805	arg1	composition					1807:1817	the chemical composition	1794:1817	the chemical composition of the cell wall of barley root cells under stress conditions	1794:1879	The presented data extend upon the knowledge on the chemical composition of the cell wall of barley root cells under stress conditions.
31234423	12	8	from	knowledge	1781:1789	arg1	composition					1807:1817	the chemical composition	1794:1817	the chemical composition of the cell wall of barley root cells under stress conditions	1794:1879	The presented data extend upon the knowledge on the chemical composition of the cell wall of barley root cells under stress conditions.
31234423	3	9	theme	pectic	408:413	arg1	epitopes					415:422	specific pectic epitopes	399:422	specific pectic epitopes	399:422	In this study, we evaluate the possible involvement of specific pectic epitopes in the cells of barley roots in response to aluminum exposure.
31234423	2	10	theme	crops	247:251	arg1	sensitive					266:274	sensitive	266:274	sensitive	266:274	Barley (Hordeum vulgare L.) is considered to be one of the crops that is most sensitive to Al, and the root cell wall is the primary target of Al toxicity.
31234423	2	10	theme	crops	247:251	arg1	one					236:238	one	236:238	one	236:238	Barley (Hordeum vulgare L.) is considered to be one of the crops that is most sensitive to Al, and the root cell wall is the primary target of Al toxicity.
31234423	2	10	theme	crops	247:251	arg1	crops					247:251	the crops	243:251	the crops that is most sensitive to Al	243:280	Barley (Hordeum vulgare L.) is considered to be one of the crops that is most sensitive to Al, and the root cell wall is the primary target of Al toxicity.
31234423	9	11	theme	cell	1476:1479	arg1	walls					1481:1485	newly arising cell walls	1462:1485	newly arising cell walls	1462:1485	Based on the presented results, it was concluded that methyl-esterified HG can be a marker for newly arising cell walls.
31234423	7	12	with	pectins	991:997	arg1	sidechains					1013:1022	galactan sidechains	1004:1022	galactan sidechains	1004:1022	In the Al-treated roots, pectins with galactan sidechains were detected with a visually lower fluorescence intensity than in the control roots while pectins with arabinan sidechains were abundantly present.
31234423	11	13	theme	root	1602:1605	arg1	diameter					1607:1614	root diameter	1602:1614	root diameter	1602:1614	Among them, an increase in root diameter, shortening of root cap, and increase in the size of rhizodermal cells and divisions of exodermal and cortex cells were observed.
31234423	13	14	theme	cells	1898:1902	arg1	response					1886:1893	The response	1882:1893	The response of cells to Al	1882:1908	The response of cells to Al can be expressed by the specific distribution of pectins in the cell wall and, thus, enables the knowledge on Al toxicity to be extended by explaining the mechanism by which Al inhibits root elongation.
31234423	3	15	theme	roots	447:451	arg1	cells					431:435	the cells	427:435	the cells of barley roots in response to aluminum exposure	427:484	In this study, we evaluate the possible involvement of specific pectic epitopes in the cells of barley roots in response to aluminum exposure.
31234423	11	16	from	increase	1590:1597	arg1	diameter					1607:1614	root diameter	1602:1614	root diameter	1602:1614	Among them, an increase in root diameter, shortening of root cap, and increase in the size of rhizodermal cells and divisions of exodermal and cortex cells were observed.
31234423	11	16	from	increase	1590:1597	arg1	size					1661:1664	the size	1657:1664	the size of rhizodermal cells	1657:1685	Among them, an increase in root diameter, shortening of root cap, and increase in the size of rhizodermal cells and divisions of exodermal and cortex cells were observed.
31234423	11	16	from	increase	1590:1597	arg1	divisions					1691:1699	divisions	1691:1699	divisions of exodermal and cortex cells	1691:1729	Among them, an increase in root diameter, shortening of root cap, and increase in the size of rhizodermal cells and divisions of exodermal and cortex cells were observed.
31234423	11	17	theme	cap	1636:1638	arg1	shortening					1617:1626	shortening	1617:1626	shortening of root cap	1617:1638	Among them, an increase in root diameter, shortening of root cap, and increase in the size of rhizodermal cells and divisions of exodermal and cortex cells were observed.
31234423	11	17	theme	cap	1636:1638	arg1	increase					1590:1597	an increase	1587:1597	an increase in root diameter	1587:1614	Among them, an increase in root diameter, shortening of root cap, and increase in the size of rhizodermal cells and divisions of exodermal and cortex cells were observed.
31234423	11	17	theme	cap	1636:1638	arg1	increase					1645:1652	increase	1645:1652	increase in the size of rhizodermal cells and divisions of exodermal and cortex cells	1645:1729	Among them, an increase in root diameter, shortening of root cap, and increase in the size of rhizodermal cells and divisions of exodermal and cortex cells were observed.
31234423	5	18	theme	barley	711:716	arg1	roots					718:722	barley roots	711:722	barley roots that had been grown in acidic conditions	711:763	Since Al becomes available and toxic to plants in acidic soils, we performed our analyses on barley roots that had been grown in acidic conditions (pH 4.0) with and without Al and in control conditions (pH 6.0).
31234423	12	19	theme	presented	1750:1758	arg1	data					1760:1763	The presented data	1746:1763	The presented data	1746:1763	The presented data extend upon the knowledge on the chemical composition of the cell wall of barley root cells under stress conditions.
31234423	3	20	theme	aluminum	468:475	arg1	exposure					477:484	aluminum exposure	468:484	aluminum exposure	468:484	In this study, we evaluate the possible involvement of specific pectic epitopes in the cells of barley roots in response to aluminum exposure.
31234423	1	21	from	production	160:169	arg1	soils					181:185	acidic soils	174:185	acidic soils	174:185	Aluminum (Al) is one of the most important crust elements causing reduced plant production in acidic soils.
31234423	12	22	theme	root	1846:1849	arg1	cells					1851:1855	barley root cells	1839:1855	barley root cells under stress conditions	1839:1879	The presented data extend upon the knowledge on the chemical composition of the cell wall of barley root cells under stress conditions.
31234423	4	23	theme	immunocytochemical	589:606	arg1	approach					608:615	an immunocytochemical approach	586:615	an immunocytochemical approach	586:615	We targeted four different pectic epitopes recognized by LM5, LM6, LM19, and LM20 antibodies using an immunocytochemical approach.
31234423	10	24	located	detected	1528:1535	arg2	changes					1515:1521	histological changes	1502:1521	histological changes	1502:1521	Additionally, histological changes were detected in the roots grown under Al exposure.
31234423	10	24	located	detected	1528:1535	arg1	roots					1544:1548	the roots	1540:1548	the roots grown under Al exposure	1540:1572	Additionally, histological changes were detected in the roots grown under Al exposure.
31234423	13	25	dep	expressed	1917:1925	arg1	enables					1995:2001	enables	1995:2001	enables the knowledge on Al toxicity to be extended by explaining the mechanism by which Al inhibits root elongation	1995:2110	The response of cells to Al can be expressed by the specific distribution of pectins in the cell wall and, thus, enables the knowledge on Al toxicity to be extended by explaining the mechanism by which Al inhibits root elongation.
31234423	7	26	theme	lower	1054:1058	arg1	intensity					1073:1081	a visually lower fluorescence intensity	1043:1081	a visually lower fluorescence intensity than in the control roots	1043:1107	In the Al-treated roots, pectins with galactan sidechains were detected with a visually lower fluorescence intensity than in the control roots while pectins with arabinan sidechains were abundantly present.
31234423	4	27	theme	LM20	564:567	arg1	antibodies					569:578	LM20 antibodies	564:578	LM20 antibodies	564:578	We targeted four different pectic epitopes recognized by LM5, LM6, LM19, and LM20 antibodies using an immunocytochemical approach.
31234423	0	28	theme	Barley	66:71	arg1	Roots					73:77	Barley Roots	66:77	Barley Roots	66:77	Aluminum Alters the Histology and Pectin Cell Wall Composition of Barley Roots.
31234423	1	29	theme	crust	123:127	arg1	elements					129:136	the most important crust elements	104:136	the most important crust elements causing reduced plant production in acidic soils	104:185	Aluminum (Al) is one of the most important crust elements causing reduced plant production in acidic soils.
31234423	6	30	dep	presence	861:868	arg1	the					857:859	the	857:859	the	857:859	Differences connected with the presence and distribution of the pectic epitopes between the control and Al-treated roots were observed.
31234423	9	31	theme	presented	1380:1388	arg1	results					1390:1396	the presented results	1376:1396	the presented results	1376:1396	Based on the presented results, it was concluded that methyl-esterified HG can be a marker for newly arising cell walls.
31234423	8	32	located	present	1338:1344	arg2	HGs					1329:1331	methyl-esterified HGs	1311:1331	methyl-esterified HGs	1311:1331	Furthermore, esterified homogalacturonans (HGs) were present with a visually higher fluorescence intensity compared to the control, while methyl-esterified HGs were present in a similar amount.
31234423	8	32	located	present	1338:1344	arg1	amount					1359:1364	a similar amount	1349:1364	a similar amount	1349:1364	Furthermore, esterified homogalacturonans (HGs) were present with a visually higher fluorescence intensity compared to the control, while methyl-esterified HGs were present in a similar amount.
31234423	6	33	theme	pectic	894:899	arg1	epitopes					901:908	the pectic epitopes	890:908	the pectic epitopes between the control and Al-treated roots	890:949	Differences connected with the presence and distribution of the pectic epitopes between the control and Al-treated roots were observed.
31234423	11	34	from	increase	1645:1652	arg1	divisions					1691:1699	divisions	1691:1699	divisions of exodermal and cortex cells	1691:1729	Among them, an increase in root diameter, shortening of root cap, and increase in the size of rhizodermal cells and divisions of exodermal and cortex cells were observed.
31234423	11	34	from	increase	1645:1652	arg1	size					1661:1664	the size	1657:1664	the size of rhizodermal cells	1657:1685	Among them, an increase in root diameter, shortening of root cap, and increase in the size of rhizodermal cells and divisions of exodermal and cortex cells were observed.
31234423	11	34	from	increase	1645:1652	arg1	diameter					1607:1614	root diameter	1602:1614	root diameter	1602:1614	Among them, an increase in root diameter, shortening of root cap, and increase in the size of rhizodermal cells and divisions of exodermal and cortex cells were observed.
31234423	2	35	theme	cell	296:299	arg1	wall					301:304	the root cell wall	287:304	the root cell wall	287:304	Barley (Hordeum vulgare L.) is considered to be one of the crops that is most sensitive to Al, and the root cell wall is the primary target of Al toxicity.
31234423	2	35	theme	cell	296:299	arg1	target					321:326	the primary target	309:326	the primary target of Al toxicity	309:341	Barley (Hordeum vulgare L.) is considered to be one of the crops that is most sensitive to Al, and the root cell wall is the primary target of Al toxicity.
31234423	2	36	theme	toxicity	334:341	arg1	wall					301:304	the root cell wall	287:304	the root cell wall	287:304	Barley (Hordeum vulgare L.) is considered to be one of the crops that is most sensitive to Al, and the root cell wall is the primary target of Al toxicity.
31234423	2	36	theme	toxicity	334:341	arg1	target					321:326	the primary target	309:326	the primary target of Al toxicity	309:341	Barley (Hordeum vulgare L.) is considered to be one of the crops that is most sensitive to Al, and the root cell wall is the primary target of Al toxicity.
31234423	8	37	from	present	1338:1344	arg1	amount					1359:1364	a similar amount	1349:1364	a similar amount	1349:1364	Furthermore, esterified homogalacturonans (HGs) were present with a visually higher fluorescence intensity compared to the control, while methyl-esterified HGs were present in a similar amount.
31234423	10	38	theme	Al	1562:1563	arg1	exposure					1565:1572	Al exposure	1562:1572	Al exposure	1562:1572	Additionally, histological changes were detected in the roots grown under Al exposure.
31234423	2	39	theme	primary	313:319	arg1	wall					301:304	the root cell wall	287:304	the root cell wall	287:304	Barley (Hordeum vulgare L.) is considered to be one of the crops that is most sensitive to Al, and the root cell wall is the primary target of Al toxicity.
31234423	2	39	theme	primary	313:319	arg1	target					321:326	the primary target	309:326	the primary target of Al toxicity	309:341	Barley (Hordeum vulgare L.) is considered to be one of the crops that is most sensitive to Al, and the root cell wall is the primary target of Al toxicity.
31234423	7	40	theme	Al-treated	973:982	arg1	roots					984:988	the Al-treated roots	969:988	the Al-treated roots	969:988	In the Al-treated roots, pectins with galactan sidechains were detected with a visually lower fluorescence intensity than in the control roots while pectins with arabinan sidechains were abundantly present.
31234423	3	41	theme	possible	375:382	arg1	involvement					384:394	the possible involvement	371:394	the possible involvement of specific pectic epitopes in the cells of barley roots in response to aluminum exposure	371:484	In this study, we evaluate the possible involvement of specific pectic epitopes in the cells of barley roots in response to aluminum exposure.
31234423	5	42	theme	acidic	668:673	arg1	soils					675:679	acidic soils	668:679	acidic soils	668:679	Since Al becomes available and toxic to plants in acidic soils, we performed our analyses on barley roots that had been grown in acidic conditions (pH 4.0) with and without Al and in control conditions (pH 6.0).
31234423	13	43	theme	cell	1974:1977	arg1	wall					1979:1982	the cell wall	1970:1982	the cell wall	1970:1982	The response of cells to Al can be expressed by the specific distribution of pectins in the cell wall and, thus, enables the knowledge on Al toxicity to be extended by explaining the mechanism by which Al inhibits root elongation.
31234423	11	44	dep	exodermal	1704:1712	arg1	cells					1725:1729	cells	1725:1729	cells	1725:1729	Among them, an increase in root diameter, shortening of root cap, and increase in the size of rhizodermal cells and divisions of exodermal and cortex cells were observed.
31234423	8	45	theme	fluorescence	1257:1268	arg1	intensity					1270:1278	a visually higher fluorescence intensity	1239:1278	a visually higher fluorescence intensity compared to the control	1239:1302	Furthermore, esterified homogalacturonans (HGs) were present with a visually higher fluorescence intensity compared to the control, while methyl-esterified HGs were present in a similar amount.
31234423	13	46	theme	specific	1934:1941	arg1	distribution					1943:1954	the specific distribution	1930:1954	the specific distribution of pectins in the cell wall	1930:1982	The response of cells to Al can be expressed by the specific distribution of pectins in the cell wall and, thus, enables the knowledge on Al toxicity to be extended by explaining the mechanism by which Al inhibits root elongation.
31234423	13	47	theme	root	2096:2099	arg1	elongation					2101:2110	root elongation	2096:2110	root elongation	2096:2110	The response of cells to Al can be expressed by the specific distribution of pectins in the cell wall and, thus, enables the knowledge on Al toxicity to be extended by explaining the mechanism by which Al inhibits root elongation.
31234423	7	48	theme	control	1095:1101	arg1	roots					1103:1107	the control roots	1091:1107	the control roots	1091:1107	In the Al-treated roots, pectins with galactan sidechains were detected with a visually lower fluorescence intensity than in the control roots while pectins with arabinan sidechains were abundantly present.
31234423	11	49	theme	rhizodermal	1669:1679	arg1	cells					1681:1685	rhizodermal cells	1669:1685	rhizodermal cells	1669:1685	Among them, an increase in root diameter, shortening of root cap, and increase in the size of rhizodermal cells and divisions of exodermal and cortex cells were observed.
31234423	5	50	dep	performed	685:693	arg1	pH					766:767	pH 4.0	766:771	pH 4.0	766:771	Since Al becomes available and toxic to plants in acidic soils, we performed our analyses on barley roots that had been grown in acidic conditions (pH 4.0) with and without Al and in control conditions (pH 6.0).
31234423	5	51	theme	acidic	747:752	arg1	conditions					754:763	acidic conditions	747:763	acidic conditions	747:763	Since Al becomes available and toxic to plants in acidic soils, we performed our analyses on barley roots that had been grown in acidic conditions (pH 4.0) with and without Al and in control conditions (pH 6.0).
31234423	1	52	theme	elements	129:136	arg1	one					97:99	one	97:99	one	97:99	Aluminum (Al) is one of the most important crust elements causing reduced plant production in acidic soils.
31234423	1	52	theme	elements	129:136	arg1	elements					129:136	the most important crust elements	104:136	the most important crust elements causing reduced plant production in acidic soils	104:185	Aluminum (Al) is one of the most important crust elements causing reduced plant production in acidic soils.
31234423	8	53	from	amount	1359:1364	arg1	present					1338:1344	present	1338:1344	present	1338:1344	Furthermore, esterified homogalacturonans (HGs) were present with a visually higher fluorescence intensity compared to the control, while methyl-esterified HGs were present in a similar amount.
31234423	2	54	dep	vulgare	204:210	arg1	L.					212:213	Hordeum vulgare L.	196:213	Hordeum vulgare L.	196:213	Barley (Hordeum vulgare L.) is considered to be one of the crops that is most sensitive to Al, and the root cell wall is the primary target of Al toxicity.
31234423	1	55	theme	reduced	146:152	arg1	production					160:169	reduced plant production	146:169	reduced plant production in acidic soils	146:185	Aluminum (Al) is one of the most important crust elements causing reduced plant production in acidic soils.
31234423	11	56	from	shortening	1617:1626	arg1	diameter					1607:1614	root diameter	1602:1614	root diameter	1602:1614	Among them, an increase in root diameter, shortening of root cap, and increase in the size of rhizodermal cells and divisions of exodermal and cortex cells were observed.
31234423	11	56	from	shortening	1617:1626	arg1	size					1661:1664	the size	1657:1664	the size of rhizodermal cells	1657:1685	Among them, an increase in root diameter, shortening of root cap, and increase in the size of rhizodermal cells and divisions of exodermal and cortex cells were observed.
31234423	11	56	from	shortening	1617:1626	arg1	divisions					1691:1699	divisions	1691:1699	divisions of exodermal and cortex cells	1691:1729	Among them, an increase in root diameter, shortening of root cap, and increase in the size of rhizodermal cells and divisions of exodermal and cortex cells were observed.
31234423	0	57	theme	Cell	41:44	arg1	Composition					51:61	the Histology and Pectin Cell Wall Composition	16:61	the Histology and Pectin Cell Wall Composition of Barley Roots	16:77	Aluminum Alters the Histology and Pectin Cell Wall Composition of Barley Roots.
31234423	7	58	with	pectins	1115:1121	arg1	sidechains					1137:1146	arabinan sidechains	1128:1146	arabinan sidechains	1128:1146	In the Al-treated roots, pectins with galactan sidechains were detected with a visually lower fluorescence intensity than in the control roots while pectins with arabinan sidechains were abundantly present.
31234423	3	59	from	involvement	384:394	arg1	cells					431:435	the cells	427:435	the cells of barley roots in response to aluminum exposure	427:484	In this study, we evaluate the possible involvement of specific pectic epitopes in the cells of barley roots in response to aluminum exposure.
31234423	3	60	theme	barley	440:445	arg1	roots					447:451	barley roots	440:451	barley roots	440:451	In this study, we evaluate the possible involvement of specific pectic epitopes in the cells of barley roots in response to aluminum exposure.
31234423	12	61	theme	barley	1839:1844	arg1	cells					1851:1855	barley root cells	1839:1855	barley root cells under stress conditions	1839:1879	The presented data extend upon the knowledge on the chemical composition of the cell wall of barley root cells under stress conditions.
31234423	4	62	theme	pectic	514:519	arg1	epitopes					521:528	four different pectic epitopes	499:528	four different pectic epitopes recognized by LM5, LM6, LM19, and LM20 antibodies	499:578	We targeted four different pectic epitopes recognized by LM5, LM6, LM19, and LM20 antibodies using an immunocytochemical approach.
31234423	8	63	with	present	1226:1232	arg1	intensity					1270:1278	a visually higher fluorescence intensity	1239:1278	a visually higher fluorescence intensity compared to the control	1239:1302	Furthermore, esterified homogalacturonans (HGs) were present with a visually higher fluorescence intensity compared to the control, while methyl-esterified HGs were present in a similar amount.
31234423	2	64	theme	Hordeum	196:202	arg1	Barley					188:193	Barley	188:193	Barley (Hordeum vulgare L.)	188:214	Barley (Hordeum vulgare L.) is considered to be one of the crops that is most sensitive to Al, and the root cell wall is the primary target of Al toxicity.
31234423	2	64	theme	Hordeum	196:202	arg1	vulgare					204:210	Hordeum vulgare L.	196:213	Hordeum vulgare L.	196:213	Barley (Hordeum vulgare L.) is considered to be one of the crops that is most sensitive to Al, and the root cell wall is the primary target of Al toxicity.
31234423	1	65	theme	plant	154:158	arg1	production					160:169	reduced plant production	146:169	reduced plant production in acidic soils	146:185	Aluminum (Al) is one of the most important crust elements causing reduced plant production in acidic soils.
31234423	1	66	theme	acidic	174:179	arg1	soils					181:185	acidic soils	174:185	acidic soils	174:185	Aluminum (Al) is one of the most important crust elements causing reduced plant production in acidic soils.
31234423	13	67	theme	Al	2020:2021	arg1	toxicity					2023:2030	Al toxicity	2020:2030	Al toxicity	2020:2030	The response of cells to Al can be expressed by the specific distribution of pectins in the cell wall and, thus, enables the knowledge on Al toxicity to be extended by explaining the mechanism by which Al inhibits root elongation.
31234423	8	68	theme	methyl-esterified	1311:1327	arg1	HGs					1329:1331	methyl-esterified HGs	1311:1331	methyl-esterified HGs	1311:1331	Furthermore, esterified homogalacturonans (HGs) were present with a visually higher fluorescence intensity compared to the control, while methyl-esterified HGs were present in a similar amount.
31234423	12	69	theme	cells	1851:1855	arg1	wall					1831:1834	the cell wall	1822:1834	the cell wall of barley root cells under stress conditions	1822:1879	The presented data extend upon the knowledge on the chemical composition of the cell wall of barley root cells under stress conditions.
31234423	11	70	theme	root	1631:1634	arg1	cap					1636:1638	root cap	1631:1638	root cap	1631:1638	Among them, an increase in root diameter, shortening of root cap, and increase in the size of rhizodermal cells and divisions of exodermal and cortex cells were observed.
31234423	12	71	theme	stress	1863:1868	arg1	conditions					1870:1879	stress conditions	1863:1879	stress conditions	1863:1879	The presented data extend upon the knowledge on the chemical composition of the cell wall of barley root cells under stress conditions.
31234423	12	72	theme	wall	1831:1834	arg1	composition					1807:1817	the chemical composition	1794:1817	the chemical composition of the cell wall of barley root cells under stress conditions	1794:1879	The presented data extend upon the knowledge on the chemical composition of the cell wall of barley root cells under stress conditions.
31234423	0	73	theme	Roots	73:77	arg1	Composition					51:61	the Histology and Pectin Cell Wall Composition	16:61	the Histology and Pectin Cell Wall Composition of Barley Roots	16:77	Aluminum Alters the Histology and Pectin Cell Wall Composition of Barley Roots.
31234423	1	74	theme	important	113:121	arg1	elements					129:136	the most important crust elements	104:136	the most important crust elements causing reduced plant production in acidic soils	104:185	Aluminum (Al) is one of the most important crust elements causing reduced plant production in acidic soils.
31234423	8	75	theme	similar	1351:1357	arg1	amount					1359:1364	a similar amount	1349:1364	a similar amount	1349:1364	Furthermore, esterified homogalacturonans (HGs) were present with a visually higher fluorescence intensity compared to the control, while methyl-esterified HGs were present in a similar amount.
31234423	1	76	dep	Aluminum	80:87	arg1	Al					90:91	Al	90:91	Al	90:91	Aluminum (Al) is one of the most important crust elements causing reduced plant production in acidic soils.
31234423	3	77	from	cells	431:435	arg1	response					456:463	response	456:463	response to aluminum exposure	456:484	In this study, we evaluate the possible involvement of specific pectic epitopes in the cells of barley roots in response to aluminum exposure.
31234423	5	78	from	plants	658:663	arg1	soils					675:679	acidic soils	668:679	acidic soils	668:679	Since Al becomes available and toxic to plants in acidic soils, we performed our analyses on barley roots that had been grown in acidic conditions (pH 4.0) with and without Al and in control conditions (pH 6.0).
31234423	6	79	theme	Al-treated	934:943	arg1	roots					945:949	Al-treated roots	934:949	Al-treated roots	934:949	Differences connected with the presence and distribution of the pectic epitopes between the control and Al-treated roots were observed.
31234423	7	80	theme	galactan	1004:1011	arg1	sidechains					1013:1022	galactan sidechains	1004:1022	galactan sidechains	1004:1022	In the Al-treated roots, pectins with galactan sidechains were detected with a visually lower fluorescence intensity than in the control roots while pectins with arabinan sidechains were abundantly present.
31234423	5	81	theme	control	801:807	arg1	pH					821:822	pH 6.0	821:826	pH 6.0	821:826	Since Al becomes available and toxic to plants in acidic soils, we performed our analyses on barley roots that had been grown in acidic conditions (pH 4.0) with and without Al and in control conditions (pH 6.0).
31234423	5	81	theme	control	801:807	arg1	conditions					809:818	control conditions	801:818	control conditions (pH 6.0)	801:827	Since Al becomes available and toxic to plants in acidic soils, we performed our analyses on barley roots that had been grown in acidic conditions (pH 4.0) with and without Al and in control conditions (pH 6.0).
31234423	6	82	theme	epitopes	901:908	arg1	distribution					874:885	distribution	874:885	distribution	874:885	Differences connected with the presence and distribution of the pectic epitopes between the control and Al-treated roots were observed.
31234423	6	82	theme	epitopes	901:908	arg1	presence					861:868	presence	861:868	presence	861:868	Differences connected with the presence and distribution of the pectic epitopes between the control and Al-treated roots were observed.
31234423	8	83	theme	esterified	1186:1195	arg1	HGs					1216:1218	HGs	1216:1218	HGs	1216:1218	Furthermore, esterified homogalacturonans (HGs) were present with a visually higher fluorescence intensity compared to the control, while methyl-esterified HGs were present in a similar amount.
31234423	8	83	theme	esterified	1186:1195	arg1	homogalacturonans					1197:1213	esterified homogalacturonans	1186:1213	esterified homogalacturonans (HGs)	1186:1219	Furthermore, esterified homogalacturonans (HGs) were present with a visually higher fluorescence intensity compared to the control, while methyl-esterified HGs were present in a similar amount.
31234423	11	84	theme	exodermal	1704:1712	arg1	divisions					1691:1699	divisions	1691:1699	divisions of exodermal and cortex cells	1691:1729	Among them, an increase in root diameter, shortening of root cap, and increase in the size of rhizodermal cells and divisions of exodermal and cortex cells were observed.
31234423	11	84	theme	exodermal	1704:1712	arg1	size					1661:1664	the size	1657:1664	the size of rhizodermal cells	1657:1685	Among them, an increase in root diameter, shortening of root cap, and increase in the size of rhizodermal cells and divisions of exodermal and cortex cells were observed.
31234423	2	85	theme	root	291:294	arg1	wall					301:304	the root cell wall	287:304	the root cell wall	287:304	Barley (Hordeum vulgare L.) is considered to be one of the crops that is most sensitive to Al, and the root cell wall is the primary target of Al toxicity.
31234423	2	85	theme	root	291:294	arg1	target					321:326	the primary target	309:326	the primary target of Al toxicity	309:341	Barley (Hordeum vulgare L.) is considered to be one of the crops that is most sensitive to Al, and the root cell wall is the primary target of Al toxicity.
31234423	0	86	theme	Histology	20:28	arg1	Composition					51:61	the Histology and Pectin Cell Wall Composition	16:61	the Histology and Pectin Cell Wall Composition of Barley Roots	16:77	Aluminum Alters the Histology and Pectin Cell Wall Composition of Barley Roots.
31234423	7	87	theme	arabinan	1128:1135	arg1	sidechains					1137:1146	arabinan sidechains	1128:1146	arabinan sidechains	1128:1146	In the Al-treated roots, pectins with galactan sidechains were detected with a visually lower fluorescence intensity than in the control roots while pectins with arabinan sidechains were abundantly present.
31234423	2	88	theme	Al	331:332	arg1	toxicity					334:341	Al toxicity	331:341	Al toxicity	331:341	Barley (Hordeum vulgare L.) is considered to be one of the crops that is most sensitive to Al, and the root cell wall is the primary target of Al toxicity.
31234423	10	89	theme	histological	1502:1513	arg1	changes					1515:1521	histological changes	1502:1521	histological changes	1502:1521	Additionally, histological changes were detected in the roots grown under Al exposure.
31234423	0	90	theme	Pectin	34:39	arg1	Composition					51:61	the Histology and Pectin Cell Wall Composition	16:61	the Histology and Pectin Cell Wall Composition of Barley Roots	16:77	Aluminum Alters the Histology and Pectin Cell Wall Composition of Barley Roots.
31234423	9	91	theme	methyl-esterified	1421:1437	arg1	marker					1451:1456	a marker	1449:1456	a marker for newly arising cell walls	1449:1485	Based on the presented results, it was concluded that methyl-esterified HG can be a marker for newly arising cell walls.
31234423	9	91	theme	methyl-esterified	1421:1437	arg1	HG					1439:1440	methyl-esterified HG	1421:1440	methyl-esterified HG	1421:1440	Based on the presented results, it was concluded that methyl-esterified HG can be a marker for newly arising cell walls.
31234423	7	92	located	detected	1029:1036	arg2	pectins					991:997	pectins	991:997	pectins with galactan sidechains	991:1022	In the Al-treated roots, pectins with galactan sidechains were detected with a visually lower fluorescence intensity than in the control roots while pectins with arabinan sidechains were abundantly present.
31234423	7	92	located	detected	1029:1036	arg1	roots					984:988	the Al-treated roots	969:988	the Al-treated roots	969:988	In the Al-treated roots, pectins with galactan sidechains were detected with a visually lower fluorescence intensity than in the control roots while pectins with arabinan sidechains were abundantly present.
31234423	11	93	theme	cortex	1718:1723	arg1	divisions					1691:1699	divisions	1691:1699	divisions of exodermal and cortex cells	1691:1729	Among them, an increase in root diameter, shortening of root cap, and increase in the size of rhizodermal cells and divisions of exodermal and cortex cells were observed.
31234423	11	93	theme	cortex	1718:1723	arg1	size					1661:1664	the size	1657:1664	the size of rhizodermal cells	1657:1685	Among them, an increase in root diameter, shortening of root cap, and increase in the size of rhizodermal cells and divisions of exodermal and cortex cells were observed.
31234423	0	94	theme	Wall	46:49	arg1	Composition					51:61	the Histology and Pectin Cell Wall Composition	16:61	the Histology and Pectin Cell Wall Composition of Barley Roots	16:77	Aluminum Alters the Histology and Pectin Cell Wall Composition of Barley Roots.
31234423	13	95	from	distribution	1943:1954	arg1	wall					1979:1982	the cell wall	1970:1982	the cell wall	1970:1982	The response of cells to Al can be expressed by the specific distribution of pectins in the cell wall and, thus, enables the knowledge on Al toxicity to be extended by explaining the mechanism by which Al inhibits root elongation.
31234423	8	96	theme	higher	1250:1255	arg1	intensity					1270:1278	a visually higher fluorescence intensity	1239:1278	a visually higher fluorescence intensity compared to the control	1239:1302	Furthermore, esterified homogalacturonans (HGs) were present with a visually higher fluorescence intensity compared to the control, while methyl-esterified HGs were present in a similar amount.
31234423	3	97	theme	epitopes	415:422	arg1	involvement					384:394	the possible involvement	371:394	the possible involvement of specific pectic epitopes in the cells of barley roots in response to aluminum exposure	371:484	In this study, we evaluate the possible involvement of specific pectic epitopes in the cells of barley roots in response to aluminum exposure.
31234423	4	98	theme	different	504:512	arg1	epitopes					521:528	four different pectic epitopes	499:528	four different pectic epitopes recognized by LM5, LM6, LM19, and LM20 antibodies	499:578	We targeted four different pectic epitopes recognized by LM5, LM6, LM19, and LM20 antibodies using an immunocytochemical approach.
31234423	11	99	theme	cells	1681:1685	arg1	divisions					1691:1699	divisions	1691:1699	divisions of exodermal and cortex cells	1691:1729	Among them, an increase in root diameter, shortening of root cap, and increase in the size of rhizodermal cells and divisions of exodermal and cortex cells were observed.
31234423	11	99	theme	cells	1681:1685	arg1	size					1661:1664	the size	1657:1664	the size of rhizodermal cells	1657:1685	Among them, an increase in root diameter, shortening of root cap, and increase in the size of rhizodermal cells and divisions of exodermal and cortex cells were observed.
31234423	13	100	theme	pectins	1959:1965	arg1	distribution					1943:1954	the specific distribution	1930:1954	the specific distribution of pectins in the cell wall	1930:1982	The response of cells to Al can be expressed by the specific distribution of pectins in the cell wall and, thus, enables the knowledge on Al toxicity to be extended by explaining the mechanism by which Al inhibits root elongation.
31126443	2	0	theme	pumpkin	619:625	arg1	S-Oil/α-CD					644:653	S-Oil/α-CD	644:653	S-Oil/α-CD	644:653	Four types of pasta were prepared: (i) control spaghetti (S-CTRL); (ii) spaghetti supplemented with α-CDs (S-α-CD); (iii) spaghetti supplemented with pumpkin oil (S-Oil) and (iv) spaghetti supplemented with the pumpkin oil/α-CD powder (S-Oil/α-CD).
31126443	2	0	theme	pumpkin	619:625	arg1	powder					636:641	the pumpkin oil/α-CD powder	615:641	the pumpkin oil/α-CD powder (S-Oil/α-CD)	615:654	Four types of pasta were prepared: (i) control spaghetti (S-CTRL); (ii) spaghetti supplemented with α-CDs (S-α-CD); (iii) spaghetti supplemented with pumpkin oil (S-Oil) and (iv) spaghetti supplemented with the pumpkin oil/α-CD powder (S-Oil/α-CD).
31126443	7	1	theme	S-Oil/α-CD	1183:1192	arg1	spaghetti					1194:1202	S-Oil/α-CD spaghetti	1183:1202	S-Oil/α-CD spaghetti	1183:1202	S-Oil/α-CD spaghetti might be accepted by customers, if the potential health benefits were also explained.
31126443	1	2	theme	supercritical	269:281	arg1	SC-CO2					299:304	SC-CO2	299:304	SC-CO2	299:304	The feasibility of producing durum wheat pasta enriched with a lipophilic phytocomplex, extracted using supercritical carbon dioxide (SC-CO2), from ripe pumpkin, as free oil or as ready-to-mix oil/α-cyclodextrins (α-CDs) powder, was explored.
31126443	1	2	theme	supercritical	269:281	arg1	dioxide					290:296	supercritical carbon dioxide	269:296	supercritical carbon dioxide (SC-CO2)	269:305	The feasibility of producing durum wheat pasta enriched with a lipophilic phytocomplex, extracted using supercritical carbon dioxide (SC-CO2), from ripe pumpkin, as free oil or as ready-to-mix oil/α-cyclodextrins (α-CDs) powder, was explored.
31126443	0	3	theme	free	87:90	arg1	oil					129:131	free or α-cyclodextrin chlatrated pumpkin oil	87:131	free or α-cyclodextrin chlatrated pumpkin oil	87:131	Bioactive composition and sensory evaluation of innovative spaghetti supplemented with free or α-cyclodextrin chlatrated pumpkin oil extracted by supercritical CO2.
31126443	3	4	theme	pasta	733:737	arg1	attributes					705:714	The chemical, antioxidant, textural and sensory attributes	657:714	The chemical, antioxidant, textural and sensory attributes of the different pasta	657:737	The chemical, antioxidant, textural and sensory attributes of the different pasta were evaluated and compared.
31126443	1	5	theme	carbon	283:288	arg1	SC-CO2					299:304	SC-CO2	299:304	SC-CO2	299:304	The feasibility of producing durum wheat pasta enriched with a lipophilic phytocomplex, extracted using supercritical carbon dioxide (SC-CO2), from ripe pumpkin, as free oil or as ready-to-mix oil/α-cyclodextrins (α-CDs) powder, was explored.
31126443	1	5	theme	carbon	283:288	arg1	dioxide					290:296	supercritical carbon dioxide	269:296	supercritical carbon dioxide (SC-CO2)	269:305	The feasibility of producing durum wheat pasta enriched with a lipophilic phytocomplex, extracted using supercritical carbon dioxide (SC-CO2), from ripe pumpkin, as free oil or as ready-to-mix oil/α-cyclodextrins (α-CDs) powder, was explored.
31126443	0	6	theme	chlatrated	110:119	arg1	oil					129:131	free or α-cyclodextrin chlatrated pumpkin oil	87:131	free or α-cyclodextrin chlatrated pumpkin oil	87:131	Bioactive composition and sensory evaluation of innovative spaghetti supplemented with free or α-cyclodextrin chlatrated pumpkin oil extracted by supercritical CO2.
31126443	6	7	theme	spaghetti	1145:1153	arg1	characteristics					1115:1129	poor textural and sensory characteristics	1089:1129	poor textural and sensory characteristics of the cooked spaghetti	1089:1153	Oil chlatration increased the stability of some bioactives during pasta production and ameliorated poor textural and sensory characteristics of the cooked spaghetti compared with S-Oil sample.
31126443	4	8	theme	S-Oil/α-CD	778:787	arg1	spaghetti					789:797	S-Oil/α-CD spaghetti	778:797	S-Oil/α-CD spaghetti	778:797	S-Oil and S-Oil/α-CD spaghetti were significantly enriched with phytosterols, squalene, carotenoids, tocochromanols and unsaturated fatty acids.
31126443	4	9	theme	fatty	900:904	arg1	acids					906:910	unsaturated fatty acids	888:910	unsaturated fatty acids	888:910	S-Oil and S-Oil/α-CD spaghetti were significantly enriched with phytosterols, squalene, carotenoids, tocochromanols and unsaturated fatty acids.
31126443	0	10	theme	α-cyclodextrin	95:108	arg1	oil					129:131	free or α-cyclodextrin chlatrated pumpkin oil	87:131	free or α-cyclodextrin chlatrated pumpkin oil	87:131	Bioactive composition and sensory evaluation of innovative spaghetti supplemented with free or α-cyclodextrin chlatrated pumpkin oil extracted by supercritical CO2.
31126443	1	11	theme	ready-to-mix	345:356	arg1	powder					386:391	ready-to-mix oil/α-cyclodextrins (α-CDs) powder	345:391	ready-to-mix oil/α-cyclodextrins (α-CDs) powder	345:391	The feasibility of producing durum wheat pasta enriched with a lipophilic phytocomplex, extracted using supercritical carbon dioxide (SC-CO2), from ripe pumpkin, as free oil or as ready-to-mix oil/α-cyclodextrins (α-CDs) powder, was explored.
31126443	3	12	theme	antioxidant	671:681	arg1	attributes					705:714	The chemical, antioxidant, textural and sensory attributes	657:714	The chemical, antioxidant, textural and sensory attributes of the different pasta	657:737	The chemical, antioxidant, textural and sensory attributes of the different pasta were evaluated and compared.
31126443	1	13	theme	oil/α-cyclodextrins	358:376	arg1	powder					386:391	ready-to-mix oil/α-cyclodextrins (α-CDs) powder	345:391	ready-to-mix oil/α-cyclodextrins (α-CDs) powder	345:391	The feasibility of producing durum wheat pasta enriched with a lipophilic phytocomplex, extracted using supercritical carbon dioxide (SC-CO2), from ripe pumpkin, as free oil or as ready-to-mix oil/α-cyclodextrins (α-CDs) powder, was explored.
31126443	2	14	theme	pumpkin	558:564	arg1	S-Oil					571:575	S-Oil	571:575	S-Oil	571:575	Four types of pasta were prepared: (i) control spaghetti (S-CTRL); (ii) spaghetti supplemented with α-CDs (S-α-CD); (iii) spaghetti supplemented with pumpkin oil (S-Oil) and (iv) spaghetti supplemented with the pumpkin oil/α-CD powder (S-Oil/α-CD).
31126443	2	14	theme	pumpkin	558:564	arg1	oil					566:568	pumpkin oil	558:568	pumpkin oil (S-Oil)	558:576	Four types of pasta were prepared: (i) control spaghetti (S-CTRL); (ii) spaghetti supplemented with α-CDs (S-α-CD); (iii) spaghetti supplemented with pumpkin oil (S-Oil) and (iv) spaghetti supplemented with the pumpkin oil/α-CD powder (S-Oil/α-CD).
31126443	6	15	theme	sensory	1107:1113	arg1	characteristics					1115:1129	poor textural and sensory characteristics	1089:1129	poor textural and sensory characteristics of the cooked spaghetti	1089:1153	Oil chlatration increased the stability of some bioactives during pasta production and ameliorated poor textural and sensory characteristics of the cooked spaghetti compared with S-Oil sample.
31126443	6	16	theme	Oil	990:992	arg1	chlatration					994:1004	Oil chlatration	990:1004	Oil chlatration	990:1004	Oil chlatration increased the stability of some bioactives during pasta production and ameliorated poor textural and sensory characteristics of the cooked spaghetti compared with S-Oil sample.
31126443	0	17	theme	pumpkin	121:127	arg1	oil					129:131	free or α-cyclodextrin chlatrated pumpkin oil	87:131	free or α-cyclodextrin chlatrated pumpkin oil	87:131	Bioactive composition and sensory evaluation of innovative spaghetti supplemented with free or α-cyclodextrin chlatrated pumpkin oil extracted by supercritical CO2.
31126443	2	18	theme	control	447:453	arg1	S-CTRL					466:471	S-CTRL	466:471	S-CTRL	466:471	Four types of pasta were prepared: (i) control spaghetti (S-CTRL); (ii) spaghetti supplemented with α-CDs (S-α-CD); (iii) spaghetti supplemented with pumpkin oil (S-Oil) and (iv) spaghetti supplemented with the pumpkin oil/α-CD powder (S-Oil/α-CD).
31126443	2	18	theme	control	447:453	arg1	spaghetti					455:463	spaghetti	455:463	(i) control spaghetti (S-CTRL); (ii) spaghetti supplemented with α-CDs (S-α-CD); (iii) spaghetti supplemented with pumpkin oil (S-Oil)	443:576	Four types of pasta were prepared: (i) control spaghetti (S-CTRL); (ii) spaghetti supplemented with α-CDs (S-α-CD); (iii) spaghetti supplemented with pumpkin oil (S-Oil) and (iv) spaghetti supplemented with the pumpkin oil/α-CD powder (S-Oil/α-CD).
31126443	3	19	theme	different	723:731	arg1	pasta					733:737	the different pasta	719:737	the different pasta	719:737	The chemical, antioxidant, textural and sensory attributes of the different pasta were evaluated and compared.
31126443	2	20	dep	spaghetti	530:538	arg1	iii					525:527	iii	525:527	iii	525:527	Four types of pasta were prepared: (i) control spaghetti (S-CTRL); (ii) spaghetti supplemented with α-CDs (S-α-CD); (iii) spaghetti supplemented with pumpkin oil (S-Oil) and (iv) spaghetti supplemented with the pumpkin oil/α-CD powder (S-Oil/α-CD).
31126443	1	21	theme	durum	194:198	arg1	pasta					206:210	durum wheat pasta	194:210	durum wheat pasta enriched with a lipophilic phytocomplex, extracted using supercritical carbon dioxide (SC-CO2), from ripe pumpkin, as free oil or as ready-to-mix oil/α-cyclodextrins (α-CDs) powder,	194:392	The feasibility of producing durum wheat pasta enriched with a lipophilic phytocomplex, extracted using supercritical carbon dioxide (SC-CO2), from ripe pumpkin, as free oil or as ready-to-mix oil/α-cyclodextrins (α-CDs) powder, was explored.
31126443	1	22	theme	α-CDs	379:383	arg1	powder					386:391	ready-to-mix oil/α-cyclodextrins (α-CDs) powder	345:391	ready-to-mix oil/α-cyclodextrins (α-CDs) powder	345:391	The feasibility of producing durum wheat pasta enriched with a lipophilic phytocomplex, extracted using supercritical carbon dioxide (SC-CO2), from ripe pumpkin, as free oil or as ready-to-mix oil/α-cyclodextrins (α-CDs) powder, was explored.
31126443	6	23	theme	textural	1094:1101	arg1	characteristics					1115:1129	poor textural and sensory characteristics	1089:1129	poor textural and sensory characteristics of the cooked spaghetti	1089:1153	Oil chlatration increased the stability of some bioactives during pasta production and ameliorated poor textural and sensory characteristics of the cooked spaghetti compared with S-Oil sample.
31126443	1	24	theme	wheat	200:204	arg1	pasta					206:210	durum wheat pasta	194:210	durum wheat pasta enriched with a lipophilic phytocomplex, extracted using supercritical carbon dioxide (SC-CO2), from ripe pumpkin, as free oil or as ready-to-mix oil/α-cyclodextrins (α-CDs) powder,	194:392	The feasibility of producing durum wheat pasta enriched with a lipophilic phytocomplex, extracted using supercritical carbon dioxide (SC-CO2), from ripe pumpkin, as free oil or as ready-to-mix oil/α-cyclodextrins (α-CDs) powder, was explored.
31126443	0	25	theme	Bioactive	0:8	arg1	composition					10:20	Bioactive composition	0:20	Bioactive composition	0:20	Bioactive composition and sensory evaluation of innovative spaghetti supplemented with free or α-cyclodextrin chlatrated pumpkin oil extracted by supercritical CO2.
31126443	6	26	theme	poor	1089:1092	arg1	characteristics					1115:1129	poor textural and sensory characteristics	1089:1129	poor textural and sensory characteristics of the cooked spaghetti	1089:1153	Oil chlatration increased the stability of some bioactives during pasta production and ameliorated poor textural and sensory characteristics of the cooked spaghetti compared with S-Oil sample.
31126443	0	27	theme	sensory	26:32	arg1	evaluation					34:43	sensory evaluation	26:43	sensory evaluation	26:43	Bioactive composition and sensory evaluation of innovative spaghetti supplemented with free or α-cyclodextrin chlatrated pumpkin oil extracted by supercritical CO2.
31126443	6	28	theme	cooked	1138:1143	arg1	spaghetti					1145:1153	the cooked spaghetti	1134:1153	the cooked spaghetti	1134:1153	Oil chlatration increased the stability of some bioactives during pasta production and ameliorated poor textural and sensory characteristics of the cooked spaghetti compared with S-Oil sample.
31126443	2	29	dep	spaghetti	587:595	arg1	iv					583:584	iv	583:584	iv	583:584	Four types of pasta were prepared: (i) control spaghetti (S-CTRL); (ii) spaghetti supplemented with α-CDs (S-α-CD); (iii) spaghetti supplemented with pumpkin oil (S-Oil) and (iv) spaghetti supplemented with the pumpkin oil/α-CD powder (S-Oil/α-CD).
31126443	7	30	theme	potential	1243:1251	arg1	benefits					1260:1267	the potential health benefits	1239:1267	the potential health benefits	1239:1267	S-Oil/α-CD spaghetti might be accepted by customers, if the potential health benefits were also explained.
31126443	7	31	theme	health	1253:1258	arg1	benefits					1260:1267	the potential health benefits	1239:1267	the potential health benefits	1239:1267	S-Oil/α-CD spaghetti might be accepted by customers, if the potential health benefits were also explained.
31126443	1	32	theme	ripe	313:316	arg1	pumpkin					318:324	ripe pumpkin	313:324	ripe pumpkin	313:324	The feasibility of producing durum wheat pasta enriched with a lipophilic phytocomplex, extracted using supercritical carbon dioxide (SC-CO2), from ripe pumpkin, as free oil or as ready-to-mix oil/α-cyclodextrins (α-CDs) powder, was explored.
31126443	0	33	theme	supercritical	146:158	arg1	CO2					160:162	supercritical CO2	146:162	supercritical CO2	146:162	Bioactive composition and sensory evaluation of innovative spaghetti supplemented with free or α-cyclodextrin chlatrated pumpkin oil extracted by supercritical CO2.
31126443	2	34	theme	pasta	422:426	arg1	types					413:417	Four types	408:417	Four types of pasta	408:426	Four types of pasta were prepared: (i) control spaghetti (S-CTRL); (ii) spaghetti supplemented with α-CDs (S-α-CD); (iii) spaghetti supplemented with pumpkin oil (S-Oil) and (iv) spaghetti supplemented with the pumpkin oil/α-CD powder (S-Oil/α-CD).
31126443	1	35	theme	lipophilic	228:237	arg1	phytocomplex					239:250	a lipophilic phytocomplex	226:250	a lipophilic phytocomplex	226:250	The feasibility of producing durum wheat pasta enriched with a lipophilic phytocomplex, extracted using supercritical carbon dioxide (SC-CO2), from ripe pumpkin, as free oil or as ready-to-mix oil/α-cyclodextrins (α-CDs) powder, was explored.
31126443	0	36	theme	spaghetti	59:67	arg1	composition					10:20	Bioactive composition	0:20	Bioactive composition	0:20	Bioactive composition and sensory evaluation of innovative spaghetti supplemented with free or α-cyclodextrin chlatrated pumpkin oil extracted by supercritical CO2.
31126443	0	36	theme	spaghetti	59:67	arg1	evaluation					34:43	sensory evaluation	26:43	sensory evaluation	26:43	Bioactive composition and sensory evaluation of innovative spaghetti supplemented with free or α-cyclodextrin chlatrated pumpkin oil extracted by supercritical CO2.
31126443	2	37	dep	spaghetti	480:488	arg1	ii					476:477	ii	476:477	ii	476:477	Four types of pasta were prepared: (i) control spaghetti (S-CTRL); (ii) spaghetti supplemented with α-CDs (S-α-CD); (iii) spaghetti supplemented with pumpkin oil (S-Oil) and (iv) spaghetti supplemented with the pumpkin oil/α-CD powder (S-Oil/α-CD).
31126443	2	38	dep	spaghetti	455:463	arg1	i					444:444	i	444:444	i	444:444	Four types of pasta were prepared: (i) control spaghetti (S-CTRL); (ii) spaghetti supplemented with α-CDs (S-α-CD); (iii) spaghetti supplemented with pumpkin oil (S-Oil) and (iv) spaghetti supplemented with the pumpkin oil/α-CD powder (S-Oil/α-CD).
31126443	2	38	dep	spaghetti	455:463	arg1	spaghetti					480:488	(ii) spaghetti	475:488	(i) control spaghetti (S-CTRL); (ii) spaghetti supplemented with α-CDs (S-α-CD); (iii) spaghetti supplemented with pumpkin oil (S-Oil)	443:576	Four types of pasta were prepared: (i) control spaghetti (S-CTRL); (ii) spaghetti supplemented with α-CDs (S-α-CD); (iii) spaghetti supplemented with pumpkin oil (S-Oil) and (iv) spaghetti supplemented with the pumpkin oil/α-CD powder (S-Oil/α-CD).
31126443	2	38	dep	spaghetti	455:463	arg1	spaghetti					530:538	(iii) spaghetti	524:538	(i) control spaghetti (S-CTRL); (ii) spaghetti supplemented with α-CDs (S-α-CD); (iii) spaghetti supplemented with pumpkin oil (S-Oil)	443:576	Four types of pasta were prepared: (i) control spaghetti (S-CTRL); (ii) spaghetti supplemented with α-CDs (S-α-CD); (iii) spaghetti supplemented with pumpkin oil (S-Oil) and (iv) spaghetti supplemented with the pumpkin oil/α-CD powder (S-Oil/α-CD).
31126443	6	39	theme	S-Oil	1169:1173	arg1	sample					1175:1180	S-Oil sample	1169:1180	S-Oil sample	1169:1180	Oil chlatration increased the stability of some bioactives during pasta production and ameliorated poor textural and sensory characteristics of the cooked spaghetti compared with S-Oil sample.
31126443	0	40	theme	innovative	48:57	arg1	spaghetti					59:67	innovative spaghetti	48:67	innovative spaghetti supplemented with free or α-cyclodextrin chlatrated pumpkin oil	48:131	Bioactive composition and sensory evaluation of innovative spaghetti supplemented with free or α-cyclodextrin chlatrated pumpkin oil extracted by supercritical CO2.
31126443	3	41	theme	chemical	661:668	arg1	attributes					705:714	The chemical, antioxidant, textural and sensory attributes	657:714	The chemical, antioxidant, textural and sensory attributes of the different pasta	657:737	The chemical, antioxidant, textural and sensory attributes of the different pasta were evaluated and compared.
31126443	6	42	theme	pasta	1056:1060	arg1	production					1062:1071	pasta production	1056:1071	pasta production	1056:1071	Oil chlatration increased the stability of some bioactives during pasta production and ameliorated poor textural and sensory characteristics of the cooked spaghetti compared with S-Oil sample.
31126443	3	43	theme	sensory	697:703	arg1	attributes					705:714	The chemical, antioxidant, textural and sensory attributes	657:714	The chemical, antioxidant, textural and sensory attributes of the different pasta	657:737	The chemical, antioxidant, textural and sensory attributes of the different pasta were evaluated and compared.
31126443	5	44	theme	fiber	975:979	arg1	content					981:987	fiber content	975:987	fiber content	975:987	Spaghetti containing α-CDs were slightly improved in terms of fiber content.
31126443	5	45	theme	Spaghetti	913:921	arg1	α-CDs					934:938	Spaghetti containing α-CDs	913:938	Spaghetti containing α-CDs	913:938	Spaghetti containing α-CDs were slightly improved in terms of fiber content.
31126443	6	46	theme	bioactives	1038:1047	arg1	stability					1020:1028	the stability	1016:1028	the stability of some bioactives	1016:1047	Oil chlatration increased the stability of some bioactives during pasta production and ameliorated poor textural and sensory characteristics of the cooked spaghetti compared with S-Oil sample.
31126443	4	47	theme	unsaturated	888:898	arg1	acids					906:910	unsaturated fatty acids	888:910	unsaturated fatty acids	888:910	S-Oil and S-Oil/α-CD spaghetti were significantly enriched with phytosterols, squalene, carotenoids, tocochromanols and unsaturated fatty acids.
31126443	5	48	theme	content	981:987	arg1	terms					966:970	terms	966:970	terms of fiber content	966:987	Spaghetti containing α-CDs were slightly improved in terms of fiber content.
31126443	5	49	theme	containing	923:932	arg1	α-CDs					934:938	Spaghetti containing α-CDs	913:938	Spaghetti containing α-CDs	913:938	Spaghetti containing α-CDs were slightly improved in terms of fiber content.
31126443	3	50	theme	textural	684:691	arg1	attributes					705:714	The chemical, antioxidant, textural and sensory attributes	657:714	The chemical, antioxidant, textural and sensory attributes of the different pasta	657:737	The chemical, antioxidant, textural and sensory attributes of the different pasta were evaluated and compared.
31126443	1	51	theme	free	330:333	arg1	oil					335:337	free oil	330:337	free oil	330:337	The feasibility of producing durum wheat pasta enriched with a lipophilic phytocomplex, extracted using supercritical carbon dioxide (SC-CO2), from ripe pumpkin, as free oil or as ready-to-mix oil/α-cyclodextrins (α-CDs) powder, was explored.
31126443	2	52	theme	oil/α-CD	627:634	arg1	S-Oil/α-CD					644:653	S-Oil/α-CD	644:653	S-Oil/α-CD	644:653	Four types of pasta were prepared: (i) control spaghetti (S-CTRL); (ii) spaghetti supplemented with α-CDs (S-α-CD); (iii) spaghetti supplemented with pumpkin oil (S-Oil) and (iv) spaghetti supplemented with the pumpkin oil/α-CD powder (S-Oil/α-CD).
31126443	2	52	theme	oil/α-CD	627:634	arg1	powder					636:641	the pumpkin oil/α-CD powder	615:641	the pumpkin oil/α-CD powder (S-Oil/α-CD)	615:654	Four types of pasta were prepared: (i) control spaghetti (S-CTRL); (ii) spaghetti supplemented with α-CDs (S-α-CD); (iii) spaghetti supplemented with pumpkin oil (S-Oil) and (iv) spaghetti supplemented with the pumpkin oil/α-CD powder (S-Oil/α-CD).
29748380	0	0	theme	C.	98:99	arg1	epidermis					109:117	the C. elegans epidermis	94:117	the C. elegans epidermis	94:117	CCAR-1 affects hemidesmosome biogenesis by regulating unc-52/perlecan alternative splicing in the C. elegans epidermis.
29748380	4	1	theme	transcription	550:562	arg1	CCAR-1					574:579	the transcription regulator CCAR-1	546:579	the transcription regulator CCAR-1	546:579	Here, we focus on the transcription regulator CCAR-1, identified in a previous genetic screen searching for enhancers of mutations in the conserved hemidesmosome component VAB-10A (known as plectin in mammals).
29748380	5	2	theme	CeHD	803:806	arg1	disruption					808:817	CeHD disruption	803:817	CeHD disruption	803:817	Loss of CCAR-1 function in a vab-10(e698) background results in CeHD disruption and muscle detachment from the epidermis.
29748380	1	3	theme	tissue	195:200	arg1	integrity					202:210	tissue integrity	195:210	tissue integrity	195:210	Hemidesmosomes are epithelial-specific attachment structures that maintain tissue integrity and resist tension.
29748380	7	4	theme	alternative	1217:1227	arg1	splicing					1229:1236	unc-52 alternative splicing	1210:1236	unc-52 alternative splicing	1210:1236	CCAR-1 physically interacts with HRP-2 (hnRNPR in mammals), a splicing factor known to mediate unc-52 alternative splicing to control the proportions of different UNC-52 isoforms and stabilize CeHDs.
29748380	9	5	theme	hemidesmosome	1509:1521	arg1	biogenesis					1523:1532	hemidesmosome biogenesis	1509:1532	hemidesmosome biogenesis	1509:1532	It also uncovers previously unappreciated roles of CCAR-1 in alternative splicing and hemidesmosome biogenesis, shedding new light on the mechanisms through which mammalian CCAR1 functions in tumorigenesis.
29748380	8	6	theme	post-transcriptional	1358:1377	arg1	regulation					1379:1388	post-transcriptional regulation	1358:1388	post-transcriptional regulation	1358:1388	Our discovery underlines the importance of post-transcriptional regulation in hemidesmosome reorganization.
29748380	9	7	theme	CCAR-1	1474:1479	arg1	roles					1465:1469	previously unappreciated roles	1440:1469	previously unappreciated roles of CCAR-1 in alternative splicing and hemidesmosome biogenesis	1440:1532	It also uncovers previously unappreciated roles of CCAR-1 in alternative splicing and hemidesmosome biogenesis, shedding new light on the mechanisms through which mammalian CCAR1 functions in tumorigenesis.
29748380	4	8	from	mutations	649:657	arg1	VAB-10A					700:706	the conserved hemidesmosome component VAB-10A	662:706	the conserved hemidesmosome component VAB-10A (known as plectin in mammals)	662:736	Here, we focus on the transcription regulator CCAR-1, identified in a previous genetic screen searching for enhancers of mutations in the conserved hemidesmosome component VAB-10A (known as plectin in mammals).
29748380	6	9	theme	genes	948:952	arg1	transcription					918:930	the transcription	914:930	the transcription of CeHD-related genes, but by affecting the alternative splicing of unc-52 (known as perlecan or HSPG2 in mammals), the predicted basement extracellular matrix (ECM) ligand of CeHDs	914:1112	CCAR-1 regulates CeHD biogenesis, not by controlling the transcription of CeHD-related genes, but by affecting the alternative splicing of unc-52 (known as perlecan or HSPG2 in mammals), the predicted basement extracellular matrix (ECM) ligand of CeHDs.
29748380	6	10	theme	CeHDs	1108:1112	arg1	unc-52					1000:1005	unc-52	1000:1005	unc-52 (known as perlecan or HSPG2 in mammals)	1000:1045	CCAR-1 regulates CeHD biogenesis, not by controlling the transcription of CeHD-related genes, but by affecting the alternative splicing of unc-52 (known as perlecan or HSPG2 in mammals), the predicted basement extracellular matrix (ECM) ligand of CeHDs.
29748380	6	10	theme	CeHDs	1108:1112	arg1	ligand					1098:1103	the predicted basement extracellular matrix (ECM) ligand	1048:1103	the predicted basement extracellular matrix (ECM) ligand of CeHDs	1048:1112	CCAR-1 regulates CeHD biogenesis, not by controlling the transcription of CeHD-related genes, but by affecting the alternative splicing of unc-52 (known as perlecan or HSPG2 in mammals), the predicted basement extracellular matrix (ECM) ligand of CeHDs.
29748380	4	11	theme	mutations	649:657	arg1	enhancers					636:644	enhancers	636:644	enhancers of mutations in the conserved hemidesmosome component VAB-10A (known as plectin in mammals)	636:736	Here, we focus on the transcription regulator CCAR-1, identified in a previous genetic screen searching for enhancers of mutations in the conserved hemidesmosome component VAB-10A (known as plectin in mammals).
29748380	6	12	theme	CeHD-related	935:946	arg1	genes					948:952	CeHD-related genes	935:952	CeHD-related genes	935:952	CCAR-1 regulates CeHD biogenesis, not by controlling the transcription of CeHD-related genes, but by affecting the alternative splicing of unc-52 (known as perlecan or HSPG2 in mammals), the predicted basement extracellular matrix (ECM) ligand of CeHDs.
29748380	9	13	theme	alternative	1484:1494	arg1	splicing					1496:1503	alternative splicing	1484:1503	alternative splicing	1484:1503	It also uncovers previously unappreciated roles of CCAR-1 in alternative splicing and hemidesmosome biogenesis, shedding new light on the mechanisms through which mammalian CCAR1 functions in tumorigenesis.
29748380	4	14	from	enhancers	636:644	arg1	VAB-10A					700:706	the conserved hemidesmosome component VAB-10A	662:706	the conserved hemidesmosome component VAB-10A (known as plectin in mammals)	662:736	Here, we focus on the transcription regulator CCAR-1, identified in a previous genetic screen searching for enhancers of mutations in the conserved hemidesmosome component VAB-10A (known as plectin in mammals).
29748380	6	15	from	perlecan	1017:1024	arg1	mammals					1038:1044	mammals	1038:1044	mammals	1038:1044	CCAR-1 regulates CeHD biogenesis, not by controlling the transcription of CeHD-related genes, but by affecting the alternative splicing of unc-52 (known as perlecan or HSPG2 in mammals), the predicted basement extracellular matrix (ECM) ligand of CeHDs.
29748380	8	16	from	importance	1344:1353	arg1	reorganization					1407:1420	hemidesmosome reorganization	1393:1420	hemidesmosome reorganization	1393:1420	Our discovery underlines the importance of post-transcriptional regulation in hemidesmosome reorganization.
29748380	8	17	theme	regulation	1379:1388	arg1	importance					1344:1353	the importance	1340:1353	the importance of post-transcriptional regulation in hemidesmosome reorganization	1340:1420	Our discovery underlines the importance of post-transcriptional regulation in hemidesmosome reorganization.
29748380	6	18	theme	alternative	976:986	arg1	splicing					988:995	the alternative splicing	972:995	the alternative splicing of unc-52 (known as perlecan or HSPG2 in mammals), the predicted basement extracellular matrix (ECM) ligand of CeHDs	972:1112	CCAR-1 regulates CeHD biogenesis, not by controlling the transcription of CeHD-related genes, but by affecting the alternative splicing of unc-52 (known as perlecan or HSPG2 in mammals), the predicted basement extracellular matrix (ECM) ligand of CeHDs.
29748380	9	19	theme	mammalian	1586:1594	arg1	CCAR1					1596:1600	mammalian CCAR1	1586:1600	mammalian CCAR1	1586:1600	It also uncovers previously unappreciated roles of CCAR-1 in alternative splicing and hemidesmosome biogenesis, shedding new light on the mechanisms through which mammalian CCAR1 functions in tumorigenesis.
29748380	5	20	from	epidermis	850:858	arg1	detachment					830:839	muscle detachment	823:839	muscle detachment from the epidermis	823:858	Loss of CCAR-1 function in a vab-10(e698) background results in CeHD disruption and muscle detachment from the epidermis.
29748380	5	20	from	epidermis	850:858	arg1	disruption					808:817	CeHD disruption	803:817	CeHD disruption	803:817	Loss of CCAR-1 function in a vab-10(e698) background results in CeHD disruption and muscle detachment from the epidermis.
29748380	0	21	dep	C.	98:99	arg1	elegans					101:107	elegans	101:107	elegans	101:107	CCAR-1 affects hemidesmosome biogenesis by regulating unc-52/perlecan alternative splicing in the C. elegans epidermis.
29748380	4	22	from	plectin	718:724	arg1	mammals					729:735	mammals	729:735	mammals	729:735	Here, we focus on the transcription regulator CCAR-1, identified in a previous genetic screen searching for enhancers of mutations in the conserved hemidesmosome component VAB-10A (known as plectin in mammals).
29748380	3	23	theme	ideal	487:491	arg1	model					493:497	an ideal model	484:497	an ideal model for studying hemidesmosomes	484:525	Caenorhabditiselegans hemidesmosomes (CeHDs) have a similar structure and composition to their mammalian counterparts, making C. elegans an ideal model for studying hemidesmosomes.
29748380	7	24	theme	splicing	1177:1184	arg1	factor					1186:1191	a splicing factor	1175:1191	a splicing factor known to mediate unc-52 alternative splicing to control the proportions of different UNC-52 isoforms and stabilize CeHDs	1175:1312	CCAR-1 physically interacts with HRP-2 (hnRNPR in mammals), a splicing factor known to mediate unc-52 alternative splicing to control the proportions of different UNC-52 isoforms and stabilize CeHDs.
29748380	7	24	theme	splicing	1177:1184	arg1	HRP-2					1148:1152	HRP-2	1148:1152	HRP-2 (hnRNPR in mammals)	1148:1172	CCAR-1 physically interacts with HRP-2 (hnRNPR in mammals), a splicing factor known to mediate unc-52 alternative splicing to control the proportions of different UNC-52 isoforms and stabilize CeHDs.
29748380	6	25	from	HSPG2	1029:1033	arg1	mammals					1038:1044	mammals	1038:1044	mammals	1038:1044	CCAR-1 regulates CeHD biogenesis, not by controlling the transcription of CeHD-related genes, but by affecting the alternative splicing of unc-52 (known as perlecan or HSPG2 in mammals), the predicted basement extracellular matrix (ECM) ligand of CeHDs.
29748380	8	26	theme	hemidesmosome	1393:1405	arg1	reorganization					1407:1420	hemidesmosome reorganization	1393:1420	hemidesmosome reorganization	1393:1420	Our discovery underlines the importance of post-transcriptional regulation in hemidesmosome reorganization.
29748380	5	27	from	Loss	739:742	arg1	background					781:790	a vab-10(e698) background	766:790	a vab-10(e698) background	766:790	Loss of CCAR-1 function in a vab-10(e698) background results in CeHD disruption and muscle detachment from the epidermis.
29748380	4	28	theme	genetic	607:613	arg1	screen					615:620	a previous genetic screen	596:620	a previous genetic screen searching for enhancers of mutations in the conserved hemidesmosome component VAB-10A (known as plectin in mammals)	596:736	Here, we focus on the transcription regulator CCAR-1, identified in a previous genetic screen searching for enhancers of mutations in the conserved hemidesmosome component VAB-10A (known as plectin in mammals).
29748380	5	29	theme	function	754:761	arg1	Loss					739:742	Loss	739:742	Loss of CCAR-1 function in a vab-10(e698) background	739:790	Loss of CCAR-1 function in a vab-10(e698) background results in CeHD disruption and muscle detachment from the epidermis.
29748380	6	30	theme	matrix	1085:1090	arg1	unc-52					1000:1005	unc-52	1000:1005	unc-52 (known as perlecan or HSPG2 in mammals)	1000:1045	CCAR-1 regulates CeHD biogenesis, not by controlling the transcription of CeHD-related genes, but by affecting the alternative splicing of unc-52 (known as perlecan or HSPG2 in mammals), the predicted basement extracellular matrix (ECM) ligand of CeHDs.
29748380	6	30	theme	matrix	1085:1090	arg1	ligand					1098:1103	the predicted basement extracellular matrix (ECM) ligand	1048:1103	the predicted basement extracellular matrix (ECM) ligand of CeHDs	1048:1112	CCAR-1 regulates CeHD biogenesis, not by controlling the transcription of CeHD-related genes, but by affecting the alternative splicing of unc-52 (known as perlecan or HSPG2 in mammals), the predicted basement extracellular matrix (ECM) ligand of CeHDs.
29748380	3	31	theme	mammalian	442:450	arg1	counterparts					452:463	their mammalian counterparts	436:463	their mammalian counterparts	436:463	Caenorhabditiselegans hemidesmosomes (CeHDs) have a similar structure and composition to their mammalian counterparts, making C. elegans an ideal model for studying hemidesmosomes.
29748380	6	32	theme	extracellular	1071:1083	arg1	ECM					1093:1095	ECM	1093:1095	ECM	1093:1095	CCAR-1 regulates CeHD biogenesis, not by controlling the transcription of CeHD-related genes, but by affecting the alternative splicing of unc-52 (known as perlecan or HSPG2 in mammals), the predicted basement extracellular matrix (ECM) ligand of CeHDs.
29748380	6	32	theme	extracellular	1071:1083	arg1	matrix					1085:1090	basement extracellular matrix	1062:1090	the predicted basement extracellular matrix (ECM) ligand of CeHDs	1048:1112	CCAR-1 regulates CeHD biogenesis, not by controlling the transcription of CeHD-related genes, but by affecting the alternative splicing of unc-52 (known as perlecan or HSPG2 in mammals), the predicted basement extracellular matrix (ECM) ligand of CeHDs.
29748380	6	33	theme	unc-52	1000:1005	arg1	splicing					988:995	the alternative splicing	972:995	the alternative splicing of unc-52 (known as perlecan or HSPG2 in mammals), the predicted basement extracellular matrix (ECM) ligand of CeHDs	972:1112	CCAR-1 regulates CeHD biogenesis, not by controlling the transcription of CeHD-related genes, but by affecting the alternative splicing of unc-52 (known as perlecan or HSPG2 in mammals), the predicted basement extracellular matrix (ECM) ligand of CeHDs.
29748380	9	34	from	roles	1465:1469	arg1	splicing					1496:1503	alternative splicing	1484:1503	alternative splicing	1484:1503	It also uncovers previously unappreciated roles of CCAR-1 in alternative splicing and hemidesmosome biogenesis, shedding new light on the mechanisms through which mammalian CCAR1 functions in tumorigenesis.
29748380	9	34	from	roles	1465:1469	arg1	biogenesis					1523:1532	hemidesmosome biogenesis	1509:1532	hemidesmosome biogenesis	1509:1532	It also uncovers previously unappreciated roles of CCAR-1 in alternative splicing and hemidesmosome biogenesis, shedding new light on the mechanisms through which mammalian CCAR1 functions in tumorigenesis.
29748380	4	35	from	VAB-10A	700:706	arg1	enhancers					636:644	enhancers	636:644	enhancers of mutations in the conserved hemidesmosome component VAB-10A (known as plectin in mammals)	636:736	Here, we focus on the transcription regulator CCAR-1, identified in a previous genetic screen searching for enhancers of mutations in the conserved hemidesmosome component VAB-10A (known as plectin in mammals).
29748380	6	36	theme	basement	1062:1069	arg1	ECM					1093:1095	ECM	1093:1095	ECM	1093:1095	CCAR-1 regulates CeHD biogenesis, not by controlling the transcription of CeHD-related genes, but by affecting the alternative splicing of unc-52 (known as perlecan or HSPG2 in mammals), the predicted basement extracellular matrix (ECM) ligand of CeHDs.
29748380	6	36	theme	basement	1062:1069	arg1	matrix					1085:1090	basement extracellular matrix	1062:1090	the predicted basement extracellular matrix (ECM) ligand of CeHDs	1048:1112	CCAR-1 regulates CeHD biogenesis, not by controlling the transcription of CeHD-related genes, but by affecting the alternative splicing of unc-52 (known as perlecan or HSPG2 in mammals), the predicted basement extracellular matrix (ECM) ligand of CeHDs.
29748380	5	37	theme	muscle	823:828	arg1	detachment					830:839	muscle detachment	823:839	muscle detachment from the epidermis	823:858	Loss of CCAR-1 function in a vab-10(e698) background results in CeHD disruption and muscle detachment from the epidermis.
29748380	5	38	theme	vab-10	768:773	arg1	background					781:790	a vab-10(e698) background	766:790	a vab-10(e698) background	766:790	Loss of CCAR-1 function in a vab-10(e698) background results in CeHD disruption and muscle detachment from the epidermis.
29748380	5	39	theme	e698	775:778	arg1	background					781:790	a vab-10(e698) background	766:790	a vab-10(e698) background	766:790	Loss of CCAR-1 function in a vab-10(e698) background results in CeHD disruption and muscle detachment from the epidermis.
29748380	6	40	theme	predicted	1052:1060	arg1	unc-52					1000:1005	unc-52	1000:1005	unc-52 (known as perlecan or HSPG2 in mammals)	1000:1045	CCAR-1 regulates CeHD biogenesis, not by controlling the transcription of CeHD-related genes, but by affecting the alternative splicing of unc-52 (known as perlecan or HSPG2 in mammals), the predicted basement extracellular matrix (ECM) ligand of CeHDs.
29748380	6	40	theme	predicted	1052:1060	arg1	ligand					1098:1103	the predicted basement extracellular matrix (ECM) ligand	1048:1103	the predicted basement extracellular matrix (ECM) ligand of CeHDs	1048:1112	CCAR-1 regulates CeHD biogenesis, not by controlling the transcription of CeHD-related genes, but by affecting the alternative splicing of unc-52 (known as perlecan or HSPG2 in mammals), the predicted basement extracellular matrix (ECM) ligand of CeHDs.
29748380	0	41	theme	alternative	70:80	arg1	splicing					82:89	unc-52/perlecan alternative splicing	54:89	unc-52/perlecan alternative splicing in the C. elegans epidermis	54:117	CCAR-1 affects hemidesmosome biogenesis by regulating unc-52/perlecan alternative splicing in the C. elegans epidermis.
29748380	0	42	from	splicing	82:89	arg1	epidermis					109:117	the C. elegans epidermis	94:117	the C. elegans epidermis	94:117	CCAR-1 affects hemidesmosome biogenesis by regulating unc-52/perlecan alternative splicing in the C. elegans epidermis.
29748380	7	43	dep	HRP-2	1148:1152	arg1	hnRNPR					1155:1160	hnRNPR	1155:1160	hnRNPR in mammals	1155:1171	CCAR-1 physically interacts with HRP-2 (hnRNPR in mammals), a splicing factor known to mediate unc-52 alternative splicing to control the proportions of different UNC-52 isoforms and stabilize CeHDs.
29748380	7	44	theme	isoforms	1285:1292	arg1	proportions					1253:1263	the proportions	1249:1263	the proportions of different UNC-52 isoforms	1249:1292	CCAR-1 physically interacts with HRP-2 (hnRNPR in mammals), a splicing factor known to mediate unc-52 alternative splicing to control the proportions of different UNC-52 isoforms and stabilize CeHDs.
29748380	0	45	theme	unc-52/perlecan	54:68	arg1	splicing					82:89	unc-52/perlecan alternative splicing	54:89	unc-52/perlecan alternative splicing in the C. elegans epidermis	54:117	CCAR-1 affects hemidesmosome biogenesis by regulating unc-52/perlecan alternative splicing in the C. elegans epidermis.
29748380	4	46	theme	previous	598:605	arg1	screen					615:620	a previous genetic screen	596:620	a previous genetic screen searching for enhancers of mutations in the conserved hemidesmosome component VAB-10A (known as plectin in mammals)	596:736	Here, we focus on the transcription regulator CCAR-1, identified in a previous genetic screen searching for enhancers of mutations in the conserved hemidesmosome component VAB-10A (known as plectin in mammals).
29748380	6	47	theme	affecting	962:970	arg1	transcription					918:930	the transcription	914:930	the transcription of CeHD-related genes, but by affecting the alternative splicing of unc-52 (known as perlecan or HSPG2 in mammals), the predicted basement extracellular matrix (ECM) ligand of CeHDs	914:1112	CCAR-1 regulates CeHD biogenesis, not by controlling the transcription of CeHD-related genes, but by affecting the alternative splicing of unc-52 (known as perlecan or HSPG2 in mammals), the predicted basement extracellular matrix (ECM) ligand of CeHDs.
29748380	4	48	dep	VAB-10A	700:706	arg1	known					709:713	known	709:713	known as plectin in mammals	709:735	Here, we focus on the transcription regulator CCAR-1, identified in a previous genetic screen searching for enhancers of mutations in the conserved hemidesmosome component VAB-10A (known as plectin in mammals).
29748380	1	49	theme	epithelial-specific	139:157	arg1	structures					170:179	epithelial-specific attachment structures	139:179	epithelial-specific attachment structures that maintain tissue integrity and resist tension	139:229	Hemidesmosomes are epithelial-specific attachment structures that maintain tissue integrity and resist tension.
29748380	1	49	theme	epithelial-specific	139:157	arg1	Hemidesmosomes					120:133	Hemidesmosomes	120:133	Hemidesmosomes	120:133	Hemidesmosomes are epithelial-specific attachment structures that maintain tissue integrity and resist tension.
29748380	3	50	theme	Caenorhabditiselegans	347:367	arg1	hemidesmosomes					369:382	Caenorhabditiselegans hemidesmosomes	347:382	Caenorhabditiselegans hemidesmosomes (CeHDs)	347:390	Caenorhabditiselegans hemidesmosomes (CeHDs) have a similar structure and composition to their mammalian counterparts, making C. elegans an ideal model for studying hemidesmosomes.
29748380	3	50	theme	Caenorhabditiselegans	347:367	arg1	CeHDs					385:389	CeHDs	385:389	CeHDs	385:389	Caenorhabditiselegans hemidesmosomes (CeHDs) have a similar structure and composition to their mammalian counterparts, making C. elegans an ideal model for studying hemidesmosomes.
29748380	6	51	dep	unc-52	1000:1005	arg1	known					1008:1012	known	1008:1012	known as perlecan or HSPG2 in mammals	1008:1044	CCAR-1 regulates CeHD biogenesis, not by controlling the transcription of CeHD-related genes, but by affecting the alternative splicing of unc-52 (known as perlecan or HSPG2 in mammals), the predicted basement extracellular matrix (ECM) ligand of CeHDs.
29748380	7	52	from	hnRNPR	1155:1160	arg1	mammals					1165:1171	mammals	1165:1171	mammals	1165:1171	CCAR-1 physically interacts with HRP-2 (hnRNPR in mammals), a splicing factor known to mediate unc-52 alternative splicing to control the proportions of different UNC-52 isoforms and stabilize CeHDs.
29748380	1	53	theme	attachment	159:168	arg1	structures					170:179	epithelial-specific attachment structures	139:179	epithelial-specific attachment structures that maintain tissue integrity and resist tension	139:229	Hemidesmosomes are epithelial-specific attachment structures that maintain tissue integrity and resist tension.
29748380	1	53	theme	attachment	159:168	arg1	Hemidesmosomes					120:133	Hemidesmosomes	120:133	Hemidesmosomes	120:133	Hemidesmosomes are epithelial-specific attachment structures that maintain tissue integrity and resist tension.
29748380	4	54	theme	conserved	666:674	arg1	VAB-10A					700:706	the conserved hemidesmosome component VAB-10A	662:706	the conserved hemidesmosome component VAB-10A (known as plectin in mammals)	662:736	Here, we focus on the transcription regulator CCAR-1, identified in a previous genetic screen searching for enhancers of mutations in the conserved hemidesmosome component VAB-10A (known as plectin in mammals).
29748380	5	55	theme	CCAR-1	747:752	arg1	function					754:761	CCAR-1 function	747:761	CCAR-1 function	747:761	Loss of CCAR-1 function in a vab-10(e698) background results in CeHD disruption and muscle detachment from the epidermis.
29748380	6	56	theme	CeHD	878:881	arg1	biogenesis					883:892	CeHD biogenesis	878:892	CeHD biogenesis	878:892	CCAR-1 regulates CeHD biogenesis, not by controlling the transcription of CeHD-related genes, but by affecting the alternative splicing of unc-52 (known as perlecan or HSPG2 in mammals), the predicted basement extracellular matrix (ECM) ligand of CeHDs.
29748380	2	57	theme	post-transcriptional	298:317	arg1	level					319:323	the post-transcriptional level	294:323	the post-transcriptional level	294:323	Despite their importance, how hemidesmosomes are regulated at the post-transcriptional level is poorly understood.
29748380	3	58	contain	have	392:395	arg1	hemidesmosomes					369:382	Caenorhabditiselegans hemidesmosomes	347:382	Caenorhabditiselegans hemidesmosomes (CeHDs)	347:390	Caenorhabditiselegans hemidesmosomes (CeHDs) have a similar structure and composition to their mammalian counterparts, making C. elegans an ideal model for studying hemidesmosomes.
29748380	3	58	contain	have	392:395	arg2	composition					421:431	composition	421:431	composition	421:431	Caenorhabditiselegans hemidesmosomes (CeHDs) have a similar structure and composition to their mammalian counterparts, making C. elegans an ideal model for studying hemidesmosomes.
29748380	3	58	contain	have	392:395	arg2	structure					407:415	a similar structure	397:415	a similar structure	397:415	Caenorhabditiselegans hemidesmosomes (CeHDs) have a similar structure and composition to their mammalian counterparts, making C. elegans an ideal model for studying hemidesmosomes.
29748380	3	58	contain	have	392:395	arg1	CeHDs					385:389	CeHDs	385:389	CeHDs	385:389	Caenorhabditiselegans hemidesmosomes (CeHDs) have a similar structure and composition to their mammalian counterparts, making C. elegans an ideal model for studying hemidesmosomes.
29748380	7	59	theme	UNC-52	1278:1283	arg1	isoforms					1285:1292	different UNC-52 isoforms	1268:1292	different UNC-52 isoforms	1268:1292	CCAR-1 physically interacts with HRP-2 (hnRNPR in mammals), a splicing factor known to mediate unc-52 alternative splicing to control the proportions of different UNC-52 isoforms and stabilize CeHDs.
29748380	7	60	theme	different	1268:1276	arg1	isoforms					1285:1292	different UNC-52 isoforms	1268:1292	different UNC-52 isoforms	1268:1292	CCAR-1 physically interacts with HRP-2 (hnRNPR in mammals), a splicing factor known to mediate unc-52 alternative splicing to control the proportions of different UNC-52 isoforms and stabilize CeHDs.
29748380	9	61	theme	new	1544:1546	arg1	light					1548:1552	new light	1544:1552	new light	1544:1552	It also uncovers previously unappreciated roles of CCAR-1 in alternative splicing and hemidesmosome biogenesis, shedding new light on the mechanisms through which mammalian CCAR1 functions in tumorigenesis.
29748380	4	62	theme	component	690:698	arg1	VAB-10A					700:706	the conserved hemidesmosome component VAB-10A	662:706	the conserved hemidesmosome component VAB-10A (known as plectin in mammals)	662:736	Here, we focus on the transcription regulator CCAR-1, identified in a previous genetic screen searching for enhancers of mutations in the conserved hemidesmosome component VAB-10A (known as plectin in mammals).
29748380	3	63	theme	similar	399:405	arg1	structure					407:415	a similar structure	397:415	a similar structure	397:415	Caenorhabditiselegans hemidesmosomes (CeHDs) have a similar structure and composition to their mammalian counterparts, making C. elegans an ideal model for studying hemidesmosomes.
29748380	4	64	theme	regulator	564:572	arg1	CCAR-1					574:579	the transcription regulator CCAR-1	546:579	the transcription regulator CCAR-1	546:579	Here, we focus on the transcription regulator CCAR-1, identified in a previous genetic screen searching for enhancers of mutations in the conserved hemidesmosome component VAB-10A (known as plectin in mammals).
29748380	0	65	theme	hemidesmosome	15:27	arg1	biogenesis					29:38	hemidesmosome biogenesis	15:38	hemidesmosome biogenesis	15:38	CCAR-1 affects hemidesmosome biogenesis by regulating unc-52/perlecan alternative splicing in the C. elegans epidermis.
29748380	9	66	theme	unappreciated	1451:1463	arg1	roles					1465:1469	previously unappreciated roles	1440:1469	previously unappreciated roles of CCAR-1 in alternative splicing and hemidesmosome biogenesis	1440:1532	It also uncovers previously unappreciated roles of CCAR-1 in alternative splicing and hemidesmosome biogenesis, shedding new light on the mechanisms through which mammalian CCAR1 functions in tumorigenesis.
29748380	4	67	theme	hemidesmosome	676:688	arg1	VAB-10A					700:706	the conserved hemidesmosome component VAB-10A	662:706	the conserved hemidesmosome component VAB-10A (known as plectin in mammals)	662:736	Here, we focus on the transcription regulator CCAR-1, identified in a previous genetic screen searching for enhancers of mutations in the conserved hemidesmosome component VAB-10A (known as plectin in mammals).
30387709	12	0	theme	similarity	1364:1373	arg1	level					1332:1336	the highest level	1320:1336	the highest level of 16S rRNA gene sequence similarity with Frondihabitans australicus E1HC-02T (97.11 %)	1320:1424	Phylogenetic analysis based on 16S rRNA gene sequences indicated that 13S1-3T represented a member of the family Microbacteriaceae and showed the highest level of 16S rRNA gene sequence similarity with Frondihabitans australicus E1HC-02T (97.11 %).
30387709	5	1	theme	cell-wall	581:589	arg1	peptidoglycan					591:603	The cell-wall peptidoglycan	577:603	The cell-wall peptidoglycan	577:603	The cell-wall peptidoglycan was of the B2γ type and contained d-alanine, d-glutamic acid, glycine, d-2,4-diaminobutyric acid and l-2,4-diaminobutyric acid.
30387709	12	2	theme	Frondihabitans	1380:1393	arg1	E1HC-02T					1407:1414	Frondihabitans australicus E1HC-02T	1380:1414	Frondihabitans australicus E1HC-02T (97.11 %)	1380:1424	Phylogenetic analysis based on 16S rRNA gene sequences indicated that 13S1-3T represented a member of the family Microbacteriaceae and showed the highest level of 16S rRNA gene sequence similarity with Frondihabitans australicus E1HC-02T (97.11 %).
30387709	12	2	theme	Frondihabitans	1380:1393	arg1	%					1423:1423	97.11 %	1417:1423	97.11 %	1417:1423	Phylogenetic analysis based on 16S rRNA gene sequences indicated that 13S1-3T represented a member of the family Microbacteriaceae and showed the highest level of 16S rRNA gene sequence similarity with Frondihabitans australicus E1HC-02T (97.11 %).
30387709	12	3	theme	highest	1324:1330	arg1	level					1332:1336	the highest level	1320:1336	the highest level of 16S rRNA gene sequence similarity with Frondihabitans australicus E1HC-02T (97.11 %)	1320:1424	Phylogenetic analysis based on 16S rRNA gene sequences indicated that 13S1-3T represented a member of the family Microbacteriaceae and showed the highest level of 16S rRNA gene sequence similarity with Frondihabitans australicus E1HC-02T (97.11 %).
30387709	12	4	theme	16S	1341:1343	arg1	similarity					1364:1373	16S rRNA gene sequence similarity	1341:1373	16S rRNA gene sequence similarity with Frondihabitans australicus E1HC-02T (97.11 %)	1341:1424	Phylogenetic analysis based on 16S rRNA gene sequences indicated that 13S1-3T represented a member of the family Microbacteriaceae and showed the highest level of 16S rRNA gene sequence similarity with Frondihabitans australicus E1HC-02T (97.11 %).
30387709	2	5	theme	Taklamakan	351:360	arg1	desert					362:367	the Taklamakan desert	347:367	the Taklamakan desert	347:367	A Gram-staining-positive, aerobic, irregular coccoid- to ovoid-shaped, non-spore-forming and motile bacterium, designated strain 13S1-3T, was isolated from a soil sample from the rhizosphere of Tamarix collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, PR China.
30387709	14	6	dep	results	1590:1596	arg1	the					1573:1575	the	1573:1575	the	1573:1575	On the basis of the results of phylogenetic, phenotypic and chemotaxonomic analyses, 13S1-3T is distinguishable from phylogenetically related genera in the family Microbacteriaceae, and represents a novel species of a new genus, for which the name Planctomonas deserti gen. nov., sp.
30387709	14	6	dep	results	1590:1596	arg1	basis					1577:1581	basis	1577:1581	basis	1577:1581	On the basis of the results of phylogenetic, phenotypic and chemotaxonomic analyses, 13S1-3T is distinguishable from phylogenetically related genera in the family Microbacteriaceae, and represents a novel species of a new genus, for which the name Planctomonas deserti gen. nov., sp.
30387709	2	7	theme	strain	254:259	arg1	13S1-3T					261:267	strain 13S1-3T	254:267	strain 13S1-3T	254:267	A Gram-staining-positive, aerobic, irregular coccoid- to ovoid-shaped, non-spore-forming and motile bacterium, designated strain 13S1-3T, was isolated from a soil sample from the rhizosphere of Tamarix collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, PR China.
30387709	12	8	theme	gene	1350:1353	arg1	similarity					1364:1373	16S rRNA gene sequence similarity	1341:1373	16S rRNA gene sequence similarity with Frondihabitans australicus E1HC-02T (97.11 %)	1341:1424	Phylogenetic analysis based on 16S rRNA gene sequences indicated that 13S1-3T represented a member of the family Microbacteriaceae and showed the highest level of 16S rRNA gene sequence similarity with Frondihabitans australicus E1HC-02T (97.11 %).
30387709	12	9	theme	Microbacteriaceae	1291:1307	arg1	member					1270:1275	a member	1268:1275	a member of the family Microbacteriaceae	1268:1307	Phylogenetic analysis based on 16S rRNA gene sequences indicated that 13S1-3T represented a member of the family Microbacteriaceae and showed the highest level of 16S rRNA gene sequence similarity with Frondihabitans australicus E1HC-02T (97.11 %).
30387709	14	10	theme	gen.	1839:1842	arg1	nov.					1844:1847	the name Planctomonas deserti gen. nov.	1809:1847	the name Planctomonas deserti gen. nov.	1809:1847	On the basis of the results of phylogenetic, phenotypic and chemotaxonomic analyses, 13S1-3T is distinguishable from phylogenetically related genera in the family Microbacteriaceae, and represents a novel species of a new genus, for which the name Planctomonas deserti gen. nov., sp.
30387709	13	11	theme	related	1518:1524	arg1	genera					1526:1531	closely related genera	1510:1531	closely related genera within the family Microbacteriaceae	1510:1567	Phylogenetic trees revealed that 13S1-3T formed a distinct lineage with respect to closely related genera within the family Microbacteriaceae.
30387709	14	12	theme	Planctomonas	1818:1829	arg1	nov.					1844:1847	the name Planctomonas deserti gen. nov.	1809:1847	the name Planctomonas deserti gen. nov.	1809:1847	On the basis of the results of phylogenetic, phenotypic and chemotaxonomic analyses, 13S1-3T is distinguishable from phylogenetically related genera in the family Microbacteriaceae, and represents a novel species of a new genus, for which the name Planctomonas deserti gen. nov., sp.
30387709	14	13	theme	chemotaxonomic	1630:1643	arg1	analyses					1645:1652	phylogenetic, phenotypic and chemotaxonomic analyses	1601:1652	phylogenetic, phenotypic and chemotaxonomic analyses	1601:1652	On the basis of the results of phylogenetic, phenotypic and chemotaxonomic analyses, 13S1-3T is distinguishable from phylogenetically related genera in the family Microbacteriaceae, and represents a novel species of a new genus, for which the name Planctomonas deserti gen. nov., sp.
30387709	2	14	theme	non-spore-forming	203:219	arg1	bacterium					232:240	A Gram-staining-positive, aerobic, irregular coccoid- to ovoid-shaped, non-spore-forming and motile bacterium	132:240	A Gram-staining-positive, aerobic, irregular coccoid- to ovoid-shaped, non-spore-forming and motile bacterium	132:240	A Gram-staining-positive, aerobic, irregular coccoid- to ovoid-shaped, non-spore-forming and motile bacterium, designated strain 13S1-3T, was isolated from a soil sample from the rhizosphere of Tamarix collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, PR China.
30387709	9	15	theme	major	921:925	arg1	diphosphatidylglycerol					945:966	diphosphatidylglycerol	945:966	diphosphatidylglycerol	945:966	The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified phospholipid.
30387709	9	15	theme	major	921:925	arg1	lipids					933:938	The major polar lipids	917:938	The major polar lipids	917:938	The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified phospholipid.
30387709	14	16	theme	phenotypic	1615:1624	arg1	analyses					1645:1652	phylogenetic, phenotypic and chemotaxonomic analyses	1601:1652	phylogenetic, phenotypic and chemotaxonomic analyses	1601:1652	On the basis of the results of phylogenetic, phenotypic and chemotaxonomic analyses, 13S1-3T is distinguishable from phylogenetically related genera in the family Microbacteriaceae, and represents a novel species of a new genus, for which the name Planctomonas deserti gen. nov., sp.
30387709	2	17	theme	ovoid-shaped	189:200	arg1	bacterium					232:240	A Gram-staining-positive, aerobic, irregular coccoid- to ovoid-shaped, non-spore-forming and motile bacterium	132:240	A Gram-staining-positive, aerobic, irregular coccoid- to ovoid-shaped, non-spore-forming and motile bacterium	132:240	A Gram-staining-positive, aerobic, irregular coccoid- to ovoid-shaped, non-spore-forming and motile bacterium, designated strain 13S1-3T, was isolated from a soil sample from the rhizosphere of Tamarix collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, PR China.
30387709	14	18	theme	phylogenetic	1601:1612	arg1	analyses					1645:1652	phylogenetic, phenotypic and chemotaxonomic analyses	1601:1652	phylogenetic, phenotypic and chemotaxonomic analyses	1601:1652	On the basis of the results of phylogenetic, phenotypic and chemotaxonomic analyses, 13S1-3T is distinguishable from phylogenetically related genera in the family Microbacteriaceae, and represents a novel species of a new genus, for which the name Planctomonas deserti gen. nov., sp.
30387709	14	19	theme	genus	1792:1796	arg1	species					1775:1781	a novel species	1767:1781	a novel species of a new genus, for which the name Planctomonas deserti gen. nov., sp	1767:1851	On the basis of the results of phylogenetic, phenotypic and chemotaxonomic analyses, 13S1-3T is distinguishable from phylogenetically related genera in the family Microbacteriaceae, and represents a novel species of a new genus, for which the name Planctomonas deserti gen. nov., sp.
30387709	5	20	theme	d-2,4-diaminobutyric	676:695	arg1	d-alanine					639:647	d-alanine	639:647	d-alanine	639:647	The cell-wall peptidoglycan was of the B2γ type and contained d-alanine, d-glutamic acid, glycine, d-2,4-diaminobutyric acid and l-2,4-diaminobutyric acid.
30387709	5	20	theme	d-2,4-diaminobutyric	676:695	arg1	acid					697:700	d-2,4-diaminobutyric acid	676:700	d-2,4-diaminobutyric acid	676:700	The cell-wall peptidoglycan was of the B2γ type and contained d-alanine, d-glutamic acid, glycine, d-2,4-diaminobutyric acid and l-2,4-diaminobutyric acid.
30387709	14	21	theme	results	1590:1596	arg1	distinguishable					1666:1680	distinguishable	1666:1680	distinguishable	1666:1680	On the basis of the results of phylogenetic, phenotypic and chemotaxonomic analyses, 13S1-3T is distinguishable from phylogenetically related genera in the family Microbacteriaceae, and represents a novel species of a new genus, for which the name Planctomonas deserti gen. nov., sp.
30387709	2	22	theme	motile	225:230	arg1	bacterium					232:240	A Gram-staining-positive, aerobic, irregular coccoid- to ovoid-shaped, non-spore-forming and motile bacterium	132:240	A Gram-staining-positive, aerobic, irregular coccoid- to ovoid-shaped, non-spore-forming and motile bacterium	132:240	A Gram-staining-positive, aerobic, irregular coccoid- to ovoid-shaped, non-spore-forming and motile bacterium, designated strain 13S1-3T, was isolated from a soil sample from the rhizosphere of Tamarix collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, PR China.
30387709	1	23	theme	Taklamakan	113:122	arg1	desert					124:129	the Taklamakan desert	109:129	the Taklamakan desert	109:129	nov., a new member of the family Microbacteriaceae isolated from soil of the Taklamakan desert.
30387709	14	24	from	genera	1712:1717	arg1	Microbacteriaceae					1733:1749	the family Microbacteriaceae	1722:1749	the family Microbacteriaceae	1722:1749	On the basis of the results of phylogenetic, phenotypic and chemotaxonomic analyses, 13S1-3T is distinguishable from phylogenetically related genera in the family Microbacteriaceae, and represents a novel species of a new genus, for which the name Planctomonas deserti gen. nov., sp.
30387709	14	24	from	genera	1712:1717	arg1	distinguishable					1666:1680	distinguishable	1666:1680	distinguishable	1666:1680	On the basis of the results of phylogenetic, phenotypic and chemotaxonomic analyses, 13S1-3T is distinguishable from phylogenetically related genera in the family Microbacteriaceae, and represents a novel species of a new genus, for which the name Planctomonas deserti gen. nov., sp.
30387709	12	25	theme	16S	1209:1211	arg1	sequences					1223:1231	16S rRNA gene sequences	1209:1231	16S rRNA gene sequences	1209:1231	Phylogenetic analysis based on 16S rRNA gene sequences indicated that 13S1-3T represented a member of the family Microbacteriaceae and showed the highest level of 16S rRNA gene sequence similarity with Frondihabitans australicus E1HC-02T (97.11 %).
30387709	13	26	theme	Phylogenetic	1427:1438	arg1	trees					1440:1444	Phylogenetic trees	1427:1444	Phylogenetic trees	1427:1444	Phylogenetic trees revealed that 13S1-3T formed a distinct lineage with respect to closely related genera within the family Microbacteriaceae.
30387709	0	27	theme	Planctomonas	0:11	arg1	nov.					26:29	Planctomonas deserti gen. nov.	0:29	Planctomonas deserti gen. nov.	0:29	Planctomonas deserti gen. nov., sp.
30387709	14	28	theme	novel	1769:1773	arg1	species					1775:1781	a novel species	1767:1781	a novel species of a new genus, for which the name Planctomonas deserti gen. nov., sp	1767:1851	On the basis of the results of phylogenetic, phenotypic and chemotaxonomic analyses, 13S1-3T is distinguishable from phylogenetically related genera in the family Microbacteriaceae, and represents a novel species of a new genus, for which the name Planctomonas deserti gen. nov., sp.
30387709	16	29	theme	49115T=CGMCC	1906:1917	arg1	1.16554T					1919:1926	=KCTC 49115T=CGMCC 1.16554T	1900:1926	=KCTC 49115T=CGMCC 1.16554T	1900:1926	The type strain is 13S1-3T (=KCTC 49115T=CGMCC 1.16554T).
30387709	16	29	theme	49115T=CGMCC	1906:1917	arg1	13S1-3T					1891:1897	13S1-3T	1891:1897	13S1-3T (=KCTC 49115T=CGMCC 1.16554T)	1891:1927	The type strain is 13S1-3T (=KCTC 49115T=CGMCC 1.16554T).
30387709	12	30	theme	gene	1218:1221	arg1	sequences					1223:1231	16S rRNA gene sequences	1209:1231	16S rRNA gene sequences	1209:1231	Phylogenetic analysis based on 16S rRNA gene sequences indicated that 13S1-3T represented a member of the family Microbacteriaceae and showed the highest level of 16S rRNA gene sequence similarity with Frondihabitans australicus E1HC-02T (97.11 %).
30387709	2	31	theme	PR	406:407	arg1	China					409:413	PR China	406:413	PR China	406:413	A Gram-staining-positive, aerobic, irregular coccoid- to ovoid-shaped, non-spore-forming and motile bacterium, designated strain 13S1-3T, was isolated from a soil sample from the rhizosphere of Tamarix collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, PR China.
30387709	2	31	theme	PR	406:407	arg1	Region					398:403	Xinjiang Uygur Autonomous Region	372:403	Xinjiang Uygur Autonomous Region	372:403	A Gram-staining-positive, aerobic, irregular coccoid- to ovoid-shaped, non-spore-forming and motile bacterium, designated strain 13S1-3T, was isolated from a soil sample from the rhizosphere of Tamarix collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, PR China.
30387709	11	32	theme	DNA	1147:1149	arg1	G+C content					1151:1161	The DNA G+C content	1143:1161	The DNA G+C content	1143:1161	The DNA G+C content was 70.4 mol%.
30387709	11	32	theme	DNA	1147:1149	arg1	%					1175:1175	70.4 mol%	1167:1175	70.4 mol%	1167:1175	The DNA G+C content was 70.4 mol%.
30387709	0	33	theme	gen.	21:24	arg1	nov.					26:29	Planctomonas deserti gen. nov.	0:29	Planctomonas deserti gen. nov.	0:29	Planctomonas deserti gen. nov., sp.
30387709	10	34	theme	whole-cell	1065:1074	arg1	anteiso-C15 					1093:1104	anteiso-C15 	1093:1104	anteiso-C15 	1093:1104	The major whole-cell fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
30387709	10	34	theme	whole-cell	1065:1074	arg1	acids					1082:1086	The major whole-cell fatty acids	1055:1086	The major whole-cell fatty acids	1055:1086	The major whole-cell fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
30387709	1	35	theme	Microbacteriaceae	69:85	arg1	member					48:53	a new member	42:53	a new member of the family Microbacteriaceae isolated from soil of the Taklamakan desert	42:129	nov., a new member of the family Microbacteriaceae isolated from soil of the Taklamakan desert.
30387709	1	35	theme	Microbacteriaceae	69:85	arg1	nov.					36:39	nov.	36:39	nov.	36:39	nov., a new member of the family Microbacteriaceae isolated from soil of the Taklamakan desert.
30387709	12	36	theme	Phylogenetic	1178:1189	arg1	analysis					1191:1198	Phylogenetic analysis	1178:1198	Phylogenetic analysis based on 16S rRNA gene sequences	1178:1231	Phylogenetic analysis based on 16S rRNA gene sequences indicated that 13S1-3T represented a member of the family Microbacteriaceae and showed the highest level of 16S rRNA gene sequence similarity with Frondihabitans australicus E1HC-02T (97.11 %).
30387709	14	37	dep	sp	1850:1851	arg1	nov.					1844:1847	the name Planctomonas deserti gen. nov.	1809:1847	the name Planctomonas deserti gen. nov.	1809:1847	On the basis of the results of phylogenetic, phenotypic and chemotaxonomic analyses, 13S1-3T is distinguishable from phylogenetically related genera in the family Microbacteriaceae, and represents a novel species of a new genus, for which the name Planctomonas deserti gen. nov., sp.
30387709	2	38	theme	Uygur	381:385	arg1	China					409:413	PR China	406:413	PR China	406:413	A Gram-staining-positive, aerobic, irregular coccoid- to ovoid-shaped, non-spore-forming and motile bacterium, designated strain 13S1-3T, was isolated from a soil sample from the rhizosphere of Tamarix collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, PR China.
30387709	2	38	theme	Uygur	381:385	arg1	Region					398:403	Xinjiang Uygur Autonomous Region	372:403	Xinjiang Uygur Autonomous Region	372:403	A Gram-staining-positive, aerobic, irregular coccoid- to ovoid-shaped, non-spore-forming and motile bacterium, designated strain 13S1-3T, was isolated from a soil sample from the rhizosphere of Tamarix collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, PR China.
30387709	6	39	theme	cell-wall	788:796	arg1	xylose					741:746	xylose	741:746	xylose	741:746	Ribose, xylose, glucose and galactose were detected as cell-wall sugars.
30387709	6	39	theme	cell-wall	788:796	arg1	Ribose					733:738	Ribose	733:738	Ribose	733:738	Ribose, xylose, glucose and galactose were detected as cell-wall sugars.
30387709	6	39	theme	cell-wall	788:796	arg1	sugars					798:803	cell-wall sugars	788:803	cell-wall sugars	788:803	Ribose, xylose, glucose and galactose were detected as cell-wall sugars.
30387709	6	39	theme	cell-wall	788:796	arg1	galactose					761:769	galactose	761:769	galactose	761:769	Ribose, xylose, glucose and galactose were detected as cell-wall sugars.
30387709	6	39	theme	cell-wall	788:796	arg1	glucose					749:755	glucose	749:755	glucose	749:755	Ribose, xylose, glucose and galactose were detected as cell-wall sugars.
30387709	3	40	theme	polyphasic	445:454	arg1	approach					456:463	a polyphasic approach	443:463	a polyphasic approach to clarify its taxonomic position	443:497	The strain was examined by a polyphasic approach to clarify its taxonomic position.
30387709	14	41	theme	family	1726:1731	arg1	Microbacteriaceae					1733:1749	the family Microbacteriaceae	1722:1749	the family Microbacteriaceae	1722:1749	On the basis of the results of phylogenetic, phenotypic and chemotaxonomic analyses, 13S1-3T is distinguishable from phylogenetically related genera in the family Microbacteriaceae, and represents a novel species of a new genus, for which the name Planctomonas deserti gen. nov., sp.
30387709	8	42	theme	predominant	856:866	arg1	MK-12					886:890	MK-12	886:890	MK-12	886:890	The predominant menaquinones were MK-12, MK-11, MK-13 and MK-10.
30387709	8	42	theme	predominant	856:866	arg1	menaquinones					868:879	The predominant menaquinones	852:879	The predominant menaquinones	852:879	The predominant menaquinones were MK-12, MK-11, MK-13 and MK-10.
30387709	13	43	theme	family	1544:1549	arg1	Microbacteriaceae					1551:1567	the family Microbacteriaceae	1540:1567	the family Microbacteriaceae	1540:1567	Phylogenetic trees revealed that 13S1-3T formed a distinct lineage with respect to closely related genera within the family Microbacteriaceae.
30387709	7	44	theme	muramic	827:833	arg1	acid					835:838	the muramic acid	823:838	the muramic acid	823:838	The acyl type of the muramic acid was acetyl.
30387709	14	45	theme	related	1704:1710	arg1	genera					1712:1717	phylogenetically related genera	1687:1717	phylogenetically related genera in the family Microbacteriaceae	1687:1749	On the basis of the results of phylogenetic, phenotypic and chemotaxonomic analyses, 13S1-3T is distinguishable from phylogenetically related genera in the family Microbacteriaceae, and represents a novel species of a new genus, for which the name Planctomonas deserti gen. nov., sp.
30387709	10	46	dep	anteiso-C15 	1093:1104	arg1	 0					1123:1124	 0	1123:1124	 0	1123:1124	The major whole-cell fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
30387709	10	46	dep	anteiso-C15 	1093:1104	arg1	iso-C16 					1130:1137	iso-C16 	1130:1137	iso-C16 	1130:1137	The major whole-cell fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
30387709	10	46	dep	anteiso-C15 	1093:1104	arg1	 0					1106:1107	 0	1106:1107	 0	1106:1107	The major whole-cell fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
30387709	10	46	dep	anteiso-C15 	1093:1104	arg1	 0					1139:1140	 0	1139:1140	 0	1139:1140	The major whole-cell fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
30387709	16	47	theme	type	1876:1879	arg1	13S1-3T					1891:1897	13S1-3T	1891:1897	13S1-3T (=KCTC 49115T=CGMCC 1.16554T)	1891:1927	The type strain is 13S1-3T (=KCTC 49115T=CGMCC 1.16554T).
30387709	16	47	theme	type	1876:1879	arg1	strain					1881:1886	The type strain	1872:1886	The type strain	1872:1886	The type strain is 13S1-3T (=KCTC 49115T=CGMCC 1.16554T).
30387709	4	48	theme	%	563:563	arg1	NaCl					571:574	0-1 % (w/v) NaCl	559:574	0-1 % (w/v) NaCl	559:574	Strain 13S1-3T grew optimally at 28-30 °C, pH 7.0 and with 0-1 % (w/v) NaCl.
30387709	1	49	attach	isolated	87:94	arg2	Microbacteriaceae					69:85	the family Microbacteriaceae	58:85	the family Microbacteriaceae isolated from soil of the Taklamakan desert	58:129	nov., a new member of the family Microbacteriaceae isolated from soil of the Taklamakan desert.
30387709	1	49	attach	isolated	87:94	arg1	soil					101:104	soil	101:104	soil of the Taklamakan desert	101:129	nov., a new member of the family Microbacteriaceae isolated from soil of the Taklamakan desert.
30387709	2	50	theme	Tamarix	326:332	arg1	rhizosphere					311:321	the rhizosphere	307:321	the rhizosphere of Tamarix collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, PR China	307:413	A Gram-staining-positive, aerobic, irregular coccoid- to ovoid-shaped, non-spore-forming and motile bacterium, designated strain 13S1-3T, was isolated from a soil sample from the rhizosphere of Tamarix collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, PR China.
30387709	7	51	theme	acyl	810:813	arg1	type					815:818	The acyl type	806:818	The acyl type of the muramic acid	806:838	The acyl type of the muramic acid was acetyl.
30387709	7	51	theme	acyl	810:813	arg1	acetyl					844:849	acetyl	844:849	acetyl	844:849	The acyl type of the muramic acid was acetyl.
30387709	12	52	theme	rRNA	1345:1348	arg1	similarity					1364:1373	16S rRNA gene sequence similarity	1341:1373	16S rRNA gene sequence similarity with Frondihabitans australicus E1HC-02T (97.11 %)	1341:1424	Phylogenetic analysis based on 16S rRNA gene sequences indicated that 13S1-3T represented a member of the family Microbacteriaceae and showed the highest level of 16S rRNA gene sequence similarity with Frondihabitans australicus E1HC-02T (97.11 %).
30387709	2	53	from	rhizosphere	311:321	arg1	sample					295:300	a soil sample	288:300	a soil sample from the rhizosphere of Tamarix collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, PR China	288:413	A Gram-staining-positive, aerobic, irregular coccoid- to ovoid-shaped, non-spore-forming and motile bacterium, designated strain 13S1-3T, was isolated from a soil sample from the rhizosphere of Tamarix collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, PR China.
30387709	12	54	theme	sequence	1355:1362	arg1	similarity					1364:1373	16S rRNA gene sequence similarity	1341:1373	16S rRNA gene sequence similarity with Frondihabitans australicus E1HC-02T (97.11 %)	1341:1424	Phylogenetic analysis based on 16S rRNA gene sequences indicated that 13S1-3T represented a member of the family Microbacteriaceae and showed the highest level of 16S rRNA gene sequence similarity with Frondihabitans australicus E1HC-02T (97.11 %).
30387709	2	55	theme	soil	290:293	arg1	sample					295:300	a soil sample	288:300	a soil sample from the rhizosphere of Tamarix collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, PR China	288:413	A Gram-staining-positive, aerobic, irregular coccoid- to ovoid-shaped, non-spore-forming and motile bacterium, designated strain 13S1-3T, was isolated from a soil sample from the rhizosphere of Tamarix collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, PR China.
30387709	12	56	theme	family	1284:1289	arg1	Microbacteriaceae					1291:1307	the family Microbacteriaceae	1280:1307	the family Microbacteriaceae	1280:1307	Phylogenetic analysis based on 16S rRNA gene sequences indicated that 13S1-3T represented a member of the family Microbacteriaceae and showed the highest level of 16S rRNA gene sequence similarity with Frondihabitans australicus E1HC-02T (97.11 %).
30387709	14	57	theme	deserti	1831:1837	arg1	nov.					1844:1847	the name Planctomonas deserti gen. nov.	1809:1847	the name Planctomonas deserti gen. nov.	1809:1847	On the basis of the results of phylogenetic, phenotypic and chemotaxonomic analyses, 13S1-3T is distinguishable from phylogenetically related genera in the family Microbacteriaceae, and represents a novel species of a new genus, for which the name Planctomonas deserti gen. nov., sp.
30387709	2	58	attach	isolated	274:281	arg2	bacterium					232:240	A Gram-staining-positive, aerobic, irregular coccoid- to ovoid-shaped, non-spore-forming and motile bacterium	132:240	A Gram-staining-positive, aerobic, irregular coccoid- to ovoid-shaped, non-spore-forming and motile bacterium	132:240	A Gram-staining-positive, aerobic, irregular coccoid- to ovoid-shaped, non-spore-forming and motile bacterium, designated strain 13S1-3T, was isolated from a soil sample from the rhizosphere of Tamarix collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, PR China.
30387709	2	58	attach	isolated	274:281	arg1	sample					295:300	a soil sample	288:300	a soil sample from the rhizosphere of Tamarix collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, PR China	288:413	A Gram-staining-positive, aerobic, irregular coccoid- to ovoid-shaped, non-spore-forming and motile bacterium, designated strain 13S1-3T, was isolated from a soil sample from the rhizosphere of Tamarix collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, PR China.
30387709	2	59	dep	ovoid-shaped	189:200	arg1	to					186:187	to	186:187	to	186:187	A Gram-staining-positive, aerobic, irregular coccoid- to ovoid-shaped, non-spore-forming and motile bacterium, designated strain 13S1-3T, was isolated from a soil sample from the rhizosphere of Tamarix collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, PR China.
30387709	14	60	theme	analyses	1645:1652	arg1	results					1590:1596	the results	1586:1596	the results of phylogenetic, phenotypic and chemotaxonomic analyses	1586:1652	On the basis of the results of phylogenetic, phenotypic and chemotaxonomic analyses, 13S1-3T is distinguishable from phylogenetically related genera in the family Microbacteriaceae, and represents a novel species of a new genus, for which the name Planctomonas deserti gen. nov., sp.
30387709	14	61	theme	name	1813:1816	arg1	nov.					1844:1847	the name Planctomonas deserti gen. nov.	1809:1847	the name Planctomonas deserti gen. nov.	1809:1847	On the basis of the results of phylogenetic, phenotypic and chemotaxonomic analyses, 13S1-3T is distinguishable from phylogenetically related genera in the family Microbacteriaceae, and represents a novel species of a new genus, for which the name Planctomonas deserti gen. nov., sp.
30387709	12	62	dep	Frondihabitans	1380:1393	arg1	australicus					1395:1405	australicus	1395:1405	australicus	1395:1405	Phylogenetic analysis based on 16S rRNA gene sequences indicated that 13S1-3T represented a member of the family Microbacteriaceae and showed the highest level of 16S rRNA gene sequence similarity with Frondihabitans australicus E1HC-02T (97.11 %).
30387709	9	63	theme	polar	927:931	arg1	diphosphatidylglycerol					945:966	diphosphatidylglycerol	945:966	diphosphatidylglycerol	945:966	The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified phospholipid.
30387709	9	63	theme	polar	927:931	arg1	lipids					933:938	The major polar lipids	917:938	The major polar lipids	917:938	The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified phospholipid.
30387709	5	64	theme	B2γ	616:618	arg1	type					620:623	the B2γ type	612:623	the B2γ type	612:623	The cell-wall peptidoglycan was of the B2γ type and contained d-alanine, d-glutamic acid, glycine, d-2,4-diaminobutyric acid and l-2,4-diaminobutyric acid.
30387709	4	65	dep	%	563:563	arg1	w/v					566:568	w/v	566:568	w/v	566:568	Strain 13S1-3T grew optimally at 28-30 °C, pH 7.0 and with 0-1 % (w/v) NaCl.
30387709	14	66	theme	new	1788:1790	arg1	genus					1792:1796	a new genus	1786:1796	a new genus	1786:1796	On the basis of the results of phylogenetic, phenotypic and chemotaxonomic analyses, 13S1-3T is distinguishable from phylogenetically related genera in the family Microbacteriaceae, and represents a novel species of a new genus, for which the name Planctomonas deserti gen. nov., sp.
30387709	1	67	theme	desert	124:129	arg1	soil					101:104	soil	101:104	soil of the Taklamakan desert	101:129	nov., a new member of the family Microbacteriaceae isolated from soil of the Taklamakan desert.
30387709	5	68	theme	d-glutamic	650:659	arg1	d-alanine					639:647	d-alanine	639:647	d-alanine	639:647	The cell-wall peptidoglycan was of the B2γ type and contained d-alanine, d-glutamic acid, glycine, d-2,4-diaminobutyric acid and l-2,4-diaminobutyric acid.
30387709	5	68	theme	d-glutamic	650:659	arg1	acid					661:664	d-glutamic acid	650:664	d-glutamic acid	650:664	The cell-wall peptidoglycan was of the B2γ type and contained d-alanine, d-glutamic acid, glycine, d-2,4-diaminobutyric acid and l-2,4-diaminobutyric acid.
30387709	9	69	theme	unidentified	995:1006	arg1	glycolipids					1008:1018	two unidentified glycolipids	991:1018	two unidentified glycolipids	991:1018	The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified phospholipid.
30387709	1	70	theme	new	44:46	arg1	member					48:53	a new member	42:53	a new member of the family Microbacteriaceae isolated from soil of the Taklamakan desert	42:129	nov., a new member of the family Microbacteriaceae isolated from soil of the Taklamakan desert.
30387709	1	70	theme	new	44:46	arg1	nov.					36:39	nov.	36:39	nov.	36:39	nov., a new member of the family Microbacteriaceae isolated from soil of the Taklamakan desert.
30387709	12	71	theme	rRNA	1213:1216	arg1	sequences					1223:1231	16S rRNA gene sequences	1209:1231	16S rRNA gene sequences	1209:1231	Phylogenetic analysis based on 16S rRNA gene sequences indicated that 13S1-3T represented a member of the family Microbacteriaceae and showed the highest level of 16S rRNA gene sequence similarity with Frondihabitans australicus E1HC-02T (97.11 %).
30387709	0	72	theme	deserti	13:19	arg1	nov.					26:29	Planctomonas deserti gen. nov.	0:29	Planctomonas deserti gen. nov.	0:29	Planctomonas deserti gen. nov., sp.
30387709	9	73	theme	unidentified	1028:1039	arg1	phospholipid					1041:1052	one unidentified phospholipid	1024:1052	one unidentified phospholipid	1024:1052	The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified phospholipid.
30387709	10	74	theme	fatty	1076:1080	arg1	anteiso-C15 					1093:1104	anteiso-C15 	1093:1104	anteiso-C15 	1093:1104	The major whole-cell fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
30387709	10	74	theme	fatty	1076:1080	arg1	acids					1082:1086	The major whole-cell fatty acids	1055:1086	The major whole-cell fatty acids	1055:1086	The major whole-cell fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
30387709	1	75	theme	family	62:67	arg1	Microbacteriaceae					69:85	the family Microbacteriaceae	58:85	the family Microbacteriaceae isolated from soil of the Taklamakan desert	58:129	nov., a new member of the family Microbacteriaceae isolated from soil of the Taklamakan desert.
30387709	12	76	with	similarity	1364:1373	arg1	E1HC-02T					1407:1414	Frondihabitans australicus E1HC-02T	1380:1414	Frondihabitans australicus E1HC-02T (97.11 %)	1380:1424	Phylogenetic analysis based on 16S rRNA gene sequences indicated that 13S1-3T represented a member of the family Microbacteriaceae and showed the highest level of 16S rRNA gene sequence similarity with Frondihabitans australicus E1HC-02T (97.11 %).
30387709	12	76	with	similarity	1364:1373	arg1	%					1423:1423	97.11 %	1417:1423	97.11 %	1417:1423	Phylogenetic analysis based on 16S rRNA gene sequences indicated that 13S1-3T represented a member of the family Microbacteriaceae and showed the highest level of 16S rRNA gene sequence similarity with Frondihabitans australicus E1HC-02T (97.11 %).
30387709	5	77	contain	contained	629:637	arg2	acid					661:664	d-glutamic acid	650:664	d-glutamic acid	650:664	The cell-wall peptidoglycan was of the B2γ type and contained d-alanine, d-glutamic acid, glycine, d-2,4-diaminobutyric acid and l-2,4-diaminobutyric acid.
30387709	5	77	contain	contained	629:637	arg2	glycine					667:673	glycine	667:673	glycine	667:673	The cell-wall peptidoglycan was of the B2γ type and contained d-alanine, d-glutamic acid, glycine, d-2,4-diaminobutyric acid and l-2,4-diaminobutyric acid.
30387709	5	77	contain	contained	629:637	arg2	acid					727:730	l-2,4-diaminobutyric acid	706:730	l-2,4-diaminobutyric acid	706:730	The cell-wall peptidoglycan was of the B2γ type and contained d-alanine, d-glutamic acid, glycine, d-2,4-diaminobutyric acid and l-2,4-diaminobutyric acid.
30387709	5	77	contain	contained	629:637	arg2	d-alanine					639:647	d-alanine	639:647	d-alanine	639:647	The cell-wall peptidoglycan was of the B2γ type and contained d-alanine, d-glutamic acid, glycine, d-2,4-diaminobutyric acid and l-2,4-diaminobutyric acid.
30387709	5	77	contain	contained	629:637	arg2	acid					697:700	d-2,4-diaminobutyric acid	676:700	d-2,4-diaminobutyric acid	676:700	The cell-wall peptidoglycan was of the B2γ type and contained d-alanine, d-glutamic acid, glycine, d-2,4-diaminobutyric acid and l-2,4-diaminobutyric acid.
30387709	5	77	contain	contained	629:637	arg1	peptidoglycan					591:603	The cell-wall peptidoglycan	577:603	The cell-wall peptidoglycan	577:603	The cell-wall peptidoglycan was of the B2γ type and contained d-alanine, d-glutamic acid, glycine, d-2,4-diaminobutyric acid and l-2,4-diaminobutyric acid.
30387709	16	78	theme	=KCTC	1900:1904	arg1	1.16554T					1919:1926	=KCTC 49115T=CGMCC 1.16554T	1900:1926	=KCTC 49115T=CGMCC 1.16554T	1900:1926	The type strain is 13S1-3T (=KCTC 49115T=CGMCC 1.16554T).
30387709	16	78	theme	=KCTC	1900:1904	arg1	13S1-3T					1891:1897	13S1-3T	1891:1897	13S1-3T (=KCTC 49115T=CGMCC 1.16554T)	1891:1927	The type strain is 13S1-3T (=KCTC 49115T=CGMCC 1.16554T).
30387709	2	79	theme	aerobic	158:164	arg1	bacterium					232:240	A Gram-staining-positive, aerobic, irregular coccoid- to ovoid-shaped, non-spore-forming and motile bacterium	132:240	A Gram-staining-positive, aerobic, irregular coccoid- to ovoid-shaped, non-spore-forming and motile bacterium	132:240	A Gram-staining-positive, aerobic, irregular coccoid- to ovoid-shaped, non-spore-forming and motile bacterium, designated strain 13S1-3T, was isolated from a soil sample from the rhizosphere of Tamarix collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, PR China.
30387709	2	80	theme	Autonomous	387:396	arg1	China					409:413	PR China	406:413	PR China	406:413	A Gram-staining-positive, aerobic, irregular coccoid- to ovoid-shaped, non-spore-forming and motile bacterium, designated strain 13S1-3T, was isolated from a soil sample from the rhizosphere of Tamarix collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, PR China.
30387709	2	80	theme	Autonomous	387:396	arg1	Region					398:403	Xinjiang Uygur Autonomous Region	372:403	Xinjiang Uygur Autonomous Region	372:403	A Gram-staining-positive, aerobic, irregular coccoid- to ovoid-shaped, non-spore-forming and motile bacterium, designated strain 13S1-3T, was isolated from a soil sample from the rhizosphere of Tamarix collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, PR China.
30387709	5	81	theme	l-2,4-diaminobutyric	706:725	arg1	d-alanine					639:647	d-alanine	639:647	d-alanine	639:647	The cell-wall peptidoglycan was of the B2γ type and contained d-alanine, d-glutamic acid, glycine, d-2,4-diaminobutyric acid and l-2,4-diaminobutyric acid.
30387709	5	81	theme	l-2,4-diaminobutyric	706:725	arg1	acid					727:730	l-2,4-diaminobutyric acid	706:730	l-2,4-diaminobutyric acid	706:730	The cell-wall peptidoglycan was of the B2γ type and contained d-alanine, d-glutamic acid, glycine, d-2,4-diaminobutyric acid and l-2,4-diaminobutyric acid.
30387709	10	82	theme	major	1059:1063	arg1	anteiso-C15 					1093:1104	anteiso-C15 	1093:1104	anteiso-C15 	1093:1104	The major whole-cell fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
30387709	10	82	theme	major	1059:1063	arg1	acids					1082:1086	The major whole-cell fatty acids	1055:1086	The major whole-cell fatty acids	1055:1086	The major whole-cell fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
30387709	2	83	theme	Gram-staining-positive	134:155	arg1	bacterium					232:240	A Gram-staining-positive, aerobic, irregular coccoid- to ovoid-shaped, non-spore-forming and motile bacterium	132:240	A Gram-staining-positive, aerobic, irregular coccoid- to ovoid-shaped, non-spore-forming and motile bacterium	132:240	A Gram-staining-positive, aerobic, irregular coccoid- to ovoid-shaped, non-spore-forming and motile bacterium, designated strain 13S1-3T, was isolated from a soil sample from the rhizosphere of Tamarix collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, PR China.
30387709	0	84	dep	sp	32:33	arg1	nov.					26:29	Planctomonas deserti gen. nov.	0:29	Planctomonas deserti gen. nov.	0:29	Planctomonas deserti gen. nov., sp.
30387709	7	85	theme	acid	835:838	arg1	type					815:818	The acyl type	806:818	The acyl type of the muramic acid	806:838	The acyl type of the muramic acid was acetyl.
30387709	7	85	theme	acid	835:838	arg1	acetyl					844:849	acetyl	844:849	acetyl	844:849	The acyl type of the muramic acid was acetyl.
30387709	13	86	theme	distinct	1477:1484	arg1	lineage					1486:1492	a distinct lineage	1475:1492	a distinct lineage	1475:1492	Phylogenetic trees revealed that 13S1-3T formed a distinct lineage with respect to closely related genera within the family Microbacteriaceae.
30387709	4	87	theme	0-1 	559:562	arg1	%					563:563	%	563:563	%	563:563	Strain 13S1-3T grew optimally at 28-30 °C, pH 7.0 and with 0-1 % (w/v) NaCl.
30387709	11	88	theme	70.4 mol	1167:1174	arg1	G+C content					1151:1161	The DNA G+C content	1143:1161	The DNA G+C content	1143:1161	The DNA G+C content was 70.4 mol%.
30387709	11	88	theme	70.4 mol	1167:1174	arg1	%					1175:1175	70.4 mol%	1167:1175	70.4 mol%	1167:1175	The DNA G+C content was 70.4 mol%.
30387709	3	89	theme	taxonomic	480:488	arg1	position					490:497	its taxonomic position	476:497	its taxonomic position	476:497	The strain was examined by a polyphasic approach to clarify its taxonomic position.
30065252	5	0	theme	D++	901:903	arg1	group					905:909	the D++ group	897:909	the D++ group	897:909	Serum 25(OH)D3 levels reduced by 63% in the D++ group and 23% in the D+ group after 6 days of DSS treatment.
30065252	4	1	theme	Microbiota	727:736	arg1	analysis					738:745	Microbiota analysis	727:745	Microbiota analysis of faecal DNA	727:759	Microbiota analysis of faecal DNA showed that the microbial composition of D++ control mice was more similar to that of DSS mice.
30065252	2	2	theme	sulphate	393:400	arg1	colitis					408:414	dextran sodium sulphate (DSS) colitis	378:414	dextran sodium sulphate (DSS) colitis in female C57Bl/6 mice	378:437	In this study, the effects of diets containing high (D++, 10,000 IU/kg), moderate (D+, 2,280 IU/kg) or no vitamin D (D-) on the severity of dextran sodium sulphate (DSS) colitis in female C57Bl/6 mice were investigated.
30065252	1	3	theme	possible	140:147	arg1	treatment					160:168	a possible adjunctive treatment	138:168	a possible adjunctive treatment to ameliorate disease severity in human inflammatory bowel disease	138:235	Vitamin D has been suggested as a possible adjunctive treatment to ameliorate disease severity in human inflammatory bowel disease.
30065252	1	3	theme	possible	140:147	arg1	D					114:114	Vitamin D	106:114	Vitamin D	106:114	Vitamin D has been suggested as a possible adjunctive treatment to ameliorate disease severity in human inflammatory bowel disease.
30065252	3	4	theme	TNF-α	709:713	arg1	expression					687:696	increased expression	677:696	increased expression of colonic TNF-α (p < 0.05)	677:724	The group on high dose vitamin D (D++) developed the most severe colitis as measured by blinded endoscopic (p < 0.001) and histologic (p < 0.05) assessment, weight loss (p < 0.001), drop in serum albumin (p = 0.05) and increased expression of colonic TNF-α (p < 0.05).
30065252	3	4	theme	TNF-α	709:713	arg1	p < 0.001					628:636	p < 0.001	628:636	p < 0.001	628:636	The group on high dose vitamin D (D++) developed the most severe colitis as measured by blinded endoscopic (p < 0.001) and histologic (p < 0.05) assessment, weight loss (p < 0.001), drop in serum albumin (p = 0.05) and increased expression of colonic TNF-α (p < 0.05).
30065252	3	4	theme	TNF-α	709:713	arg1	p < 0.001					566:574	blinded endoscopic (p < 0.001) and histologic (p < 0.05) assessment	546:612	p < 0.001	566:574	The group on high dose vitamin D (D++) developed the most severe colitis as measured by blinded endoscopic (p < 0.001) and histologic (p < 0.05) assessment, weight loss (p < 0.001), drop in serum albumin (p = 0.05) and increased expression of colonic TNF-α (p < 0.05).
30065252	3	4	theme	TNF-α	709:713	arg1	assessment					603:612	blinded endoscopic (p < 0.001) and histologic (p < 0.05) assessment	546:612	assessment	603:612	The group on high dose vitamin D (D++) developed the most severe colitis as measured by blinded endoscopic (p < 0.001) and histologic (p < 0.05) assessment, weight loss (p < 0.001), drop in serum albumin (p = 0.05) and increased expression of colonic TNF-α (p < 0.05).
30065252	3	4	theme	TNF-α	709:713	arg1	drop					640:643	drop	640:643	drop in serum albumin (p = 0.05)	640:671	The group on high dose vitamin D (D++) developed the most severe colitis as measured by blinded endoscopic (p < 0.001) and histologic (p < 0.05) assessment, weight loss (p < 0.001), drop in serum albumin (p = 0.05) and increased expression of colonic TNF-α (p < 0.05).
30065252	3	4	theme	TNF-α	709:713	arg1	loss					622:625	weight loss	615:625	weight loss (p < 0.001)	615:637	The group on high dose vitamin D (D++) developed the most severe colitis as measured by blinded endoscopic (p < 0.001) and histologic (p < 0.05) assessment, weight loss (p < 0.001), drop in serum albumin (p = 0.05) and increased expression of colonic TNF-α (p < 0.05).
30065252	0	5	theme	severe	90:95	arg1	colitis					97:103	more severe colitis	85:103	more severe colitis	85:103	High Dose Vitamin D supplementation alters faecal microbiome and predisposes mice to more severe colitis.
30065252	3	6	from	expression	687:696	arg1	p = 0.05					663:670	p = 0.05	663:670	p = 0.05	663:670	The group on high dose vitamin D (D++) developed the most severe colitis as measured by blinded endoscopic (p < 0.001) and histologic (p < 0.05) assessment, weight loss (p < 0.001), drop in serum albumin (p = 0.05) and increased expression of colonic TNF-α (p < 0.05).
30065252	3	6	from	expression	687:696	arg1	albumin					654:660	serum albumin	648:660	serum albumin (p = 0.05)	648:671	The group on high dose vitamin D (D++) developed the most severe colitis as measured by blinded endoscopic (p < 0.001) and histologic (p < 0.05) assessment, weight loss (p < 0.001), drop in serum albumin (p = 0.05) and increased expression of colonic TNF-α (p < 0.05).
30065252	2	7	theme	diets	268:272	arg1	effects					257:263	the effects	253:263	the effects of diets containing high (D++, 10,000 IU/kg), moderate (D+, 2,280 IU/kg) or no vitamin D (D-) on the severity of dextran sodium sulphate (DSS) colitis in female C57Bl/6 mice	253:437	In this study, the effects of diets containing high (D++, 10,000 IU/kg), moderate (D+, 2,280 IU/kg) or no vitamin D (D-) on the severity of dextran sodium sulphate (DSS) colitis in female C57Bl/6 mice were investigated.
30065252	3	8	theme	endoscopic	554:563	arg1	p < 0.001					566:574	blinded endoscopic (p < 0.001) and histologic (p < 0.05) assessment	546:612	p < 0.001	566:574	The group on high dose vitamin D (D++) developed the most severe colitis as measured by blinded endoscopic (p < 0.001) and histologic (p < 0.05) assessment, weight loss (p < 0.001), drop in serum albumin (p = 0.05) and increased expression of colonic TNF-α (p < 0.05).
30065252	5	9	theme	DSS	951:953	arg1	treatment					955:963	DSS treatment	951:963	DSS treatment	951:963	Serum 25(OH)D3 levels reduced by 63% in the D++ group and 23% in the D+ group after 6 days of DSS treatment.
30065252	2	10	theme	female	419:424	arg1	mice					434:437	female C57Bl/6 mice	419:437	female C57Bl/6 mice	419:437	In this study, the effects of diets containing high (D++, 10,000 IU/kg), moderate (D+, 2,280 IU/kg) or no vitamin D (D-) on the severity of dextran sodium sulphate (DSS) colitis in female C57Bl/6 mice were investigated.
30065252	3	11	theme	p < 0.05	593:600	arg1	assessment					603:612	blinded endoscopic (p < 0.001) and histologic (p < 0.05) assessment	546:612	assessment	603:612	The group on high dose vitamin D (D++) developed the most severe colitis as measured by blinded endoscopic (p < 0.001) and histologic (p < 0.05) assessment, weight loss (p < 0.001), drop in serum albumin (p = 0.05) and increased expression of colonic TNF-α (p < 0.05).
30065252	4	12	theme	DSS	847:849	arg1	mice					851:854	DSS mice	847:854	DSS mice	847:854	Microbiota analysis of faecal DNA showed that the microbial composition of D++ control mice was more similar to that of DSS mice.
30065252	5	13	from	%	917:917	arg1	group					929:933	the D+ group	922:933	the D+ group	922:933	Serum 25(OH)D3 levels reduced by 63% in the D++ group and 23% in the D+ group after 6 days of DSS treatment.
30065252	5	13	from	%	917:917	arg1	group					905:909	the D++ group	897:909	the D++ group	897:909	Serum 25(OH)D3 levels reduced by 63% in the D++ group and 23% in the D+ group after 6 days of DSS treatment.
30065252	5	14	theme	treatment	955:963	arg1	days					943:946	6 days	941:946	6 days of DSS treatment	941:963	Serum 25(OH)D3 levels reduced by 63% in the D++ group and 23% in the D+ group after 6 days of DSS treatment.
30065252	2	15	from	severity	366:373	arg1	mice					434:437	female C57Bl/6 mice	419:437	female C57Bl/6 mice	419:437	In this study, the effects of diets containing high (D++, 10,000 IU/kg), moderate (D+, 2,280 IU/kg) or no vitamin D (D-) on the severity of dextran sodium sulphate (DSS) colitis in female C57Bl/6 mice were investigated.
30065252	3	16	from	assessment	603:612	arg1	p = 0.05					663:670	p = 0.05	663:670	p = 0.05	663:670	The group on high dose vitamin D (D++) developed the most severe colitis as measured by blinded endoscopic (p < 0.001) and histologic (p < 0.05) assessment, weight loss (p < 0.001), drop in serum albumin (p = 0.05) and increased expression of colonic TNF-α (p < 0.05).
30065252	3	16	from	assessment	603:612	arg1	albumin					654:660	serum albumin	648:660	serum albumin (p = 0.05)	648:671	The group on high dose vitamin D (D++) developed the most severe colitis as measured by blinded endoscopic (p < 0.001) and histologic (p < 0.05) assessment, weight loss (p < 0.001), drop in serum albumin (p = 0.05) and increased expression of colonic TNF-α (p < 0.05).
30065252	6	17	theme	inflammatory	1204:1215	arg1	process					1217:1223	the inflammatory process	1200:1223	the inflammatory process	1200:1223	Thus, high dose vitamin D supplementation is associated with a shift to a more inflammatory faecal microbiome and increased susceptibility to colitis, with a fall in circulating vitamin D occurring as a secondary event in response to the inflammatory process.
30065252	1	18	theme	adjunctive	149:158	arg1	treatment					160:168	a possible adjunctive treatment	138:168	a possible adjunctive treatment to ameliorate disease severity in human inflammatory bowel disease	138:235	Vitamin D has been suggested as a possible adjunctive treatment to ameliorate disease severity in human inflammatory bowel disease.
30065252	1	18	theme	adjunctive	149:158	arg1	D					114:114	Vitamin D	106:114	Vitamin D	106:114	Vitamin D has been suggested as a possible adjunctive treatment to ameliorate disease severity in human inflammatory bowel disease.
30065252	2	19	theme	colitis	408:414	arg1	severity					366:373	the severity	362:373	the severity of dextran sodium sulphate (DSS) colitis in female C57Bl/6 mice	362:437	In this study, the effects of diets containing high (D++, 10,000 IU/kg), moderate (D+, 2,280 IU/kg) or no vitamin D (D-) on the severity of dextran sodium sulphate (DSS) colitis in female C57Bl/6 mice were investigated.
30065252	3	20	theme	histologic	581:590	arg1	assessment					603:612	blinded endoscopic (p < 0.001) and histologic (p < 0.05) assessment	546:612	assessment	603:612	The group on high dose vitamin D (D++) developed the most severe colitis as measured by blinded endoscopic (p < 0.001) and histologic (p < 0.05) assessment, weight loss (p < 0.001), drop in serum albumin (p = 0.05) and increased expression of colonic TNF-α (p < 0.05).
30065252	6	21	theme	D	990:990	arg1	supplementation					992:1006	high dose vitamin D supplementation	972:1006	high dose vitamin D supplementation	972:1006	Thus, high dose vitamin D supplementation is associated with a shift to a more inflammatory faecal microbiome and increased susceptibility to colitis, with a fall in circulating vitamin D occurring as a secondary event in response to the inflammatory process.
30065252	2	22	from	mice	434:437	arg1	severity					366:373	the severity	362:373	the severity of dextran sodium sulphate (DSS) colitis in female C57Bl/6 mice	362:437	In this study, the effects of diets containing high (D++, 10,000 IU/kg), moderate (D+, 2,280 IU/kg) or no vitamin D (D-) on the severity of dextran sodium sulphate (DSS) colitis in female C57Bl/6 mice were investigated.
30065252	4	23	theme	D++	802:804	arg1	mice					814:817	D++ control mice	802:817	D++ control mice	802:817	Microbiota analysis of faecal DNA showed that the microbial composition of D++ control mice was more similar to that of DSS mice.
30065252	0	24	theme	Dose	5:8	arg1	supplementation					20:34	High Dose Vitamin D supplementation	0:34	High Dose Vitamin D supplementation	0:34	High Dose Vitamin D supplementation alters faecal microbiome and predisposes mice to more severe colitis.
30065252	4	25	theme	mice	814:817	arg1	composition					787:797	the microbial composition	773:797	the microbial composition of D++ control mice	773:817	Microbiota analysis of faecal DNA showed that the microbial composition of D++ control mice was more similar to that of DSS mice.
30065252	4	25	theme	mice	814:817	arg1	similar					828:834	similar	828:834	similar	828:834	Microbiota analysis of faecal DNA showed that the microbial composition of D++ control mice was more similar to that of DSS mice.
30065252	6	26	theme	vitamin	982:988	arg1	supplementation					992:1006	high dose vitamin D supplementation	972:1006	high dose vitamin D supplementation	972:1006	Thus, high dose vitamin D supplementation is associated with a shift to a more inflammatory faecal microbiome and increased susceptibility to colitis, with a fall in circulating vitamin D occurring as a secondary event in response to the inflammatory process.
30065252	5	27	theme	D+	926:927	arg1	group					929:933	the D+ group	922:933	the D+ group	922:933	Serum 25(OH)D3 levels reduced by 63% in the D++ group and 23% in the D+ group after 6 days of DSS treatment.
30065252	4	28	theme	microbial	777:785	arg1	composition					787:797	the microbial composition	773:797	the microbial composition of D++ control mice	773:817	Microbiota analysis of faecal DNA showed that the microbial composition of D++ control mice was more similar to that of DSS mice.
30065252	4	28	theme	microbial	777:785	arg1	similar					828:834	similar	828:834	similar	828:834	Microbiota analysis of faecal DNA showed that the microbial composition of D++ control mice was more similar to that of DSS mice.
30065252	0	29	theme	High	0:3	arg1	supplementation					20:34	High Dose Vitamin D supplementation	0:34	High Dose Vitamin D supplementation	0:34	High Dose Vitamin D supplementation alters faecal microbiome and predisposes mice to more severe colitis.
30065252	3	30	theme	weight	615:620	arg1	p < 0.001					628:636	p < 0.001	628:636	p < 0.001	628:636	The group on high dose vitamin D (D++) developed the most severe colitis as measured by blinded endoscopic (p < 0.001) and histologic (p < 0.05) assessment, weight loss (p < 0.001), drop in serum albumin (p = 0.05) and increased expression of colonic TNF-α (p < 0.05).
30065252	3	30	theme	weight	615:620	arg1	loss					622:625	weight loss	615:625	weight loss (p < 0.001)	615:637	The group on high dose vitamin D (D++) developed the most severe colitis as measured by blinded endoscopic (p < 0.001) and histologic (p < 0.05) assessment, weight loss (p < 0.001), drop in serum albumin (p = 0.05) and increased expression of colonic TNF-α (p < 0.05).
30065252	3	31	from	loss	622:625	arg1	p = 0.05					663:670	p = 0.05	663:670	p = 0.05	663:670	The group on high dose vitamin D (D++) developed the most severe colitis as measured by blinded endoscopic (p < 0.001) and histologic (p < 0.05) assessment, weight loss (p < 0.001), drop in serum albumin (p = 0.05) and increased expression of colonic TNF-α (p < 0.05).
30065252	3	31	from	loss	622:625	arg1	albumin					654:660	serum albumin	648:660	serum albumin (p = 0.05)	648:671	The group on high dose vitamin D (D++) developed the most severe colitis as measured by blinded endoscopic (p < 0.001) and histologic (p < 0.05) assessment, weight loss (p < 0.001), drop in serum albumin (p = 0.05) and increased expression of colonic TNF-α (p < 0.05).
30065252	3	32	theme	high	471:474	arg1	D					489:489	high dose vitamin D	471:489	high dose vitamin D (D++)	471:495	The group on high dose vitamin D (D++) developed the most severe colitis as measured by blinded endoscopic (p < 0.001) and histologic (p < 0.05) assessment, weight loss (p < 0.001), drop in serum albumin (p = 0.05) and increased expression of colonic TNF-α (p < 0.05).
30065252	3	32	theme	high	471:474	arg1	D++					492:494	D++	492:494	D++	492:494	The group on high dose vitamin D (D++) developed the most severe colitis as measured by blinded endoscopic (p < 0.001) and histologic (p < 0.05) assessment, weight loss (p < 0.001), drop in serum albumin (p = 0.05) and increased expression of colonic TNF-α (p < 0.05).
30065252	0	33	theme	D	18:18	arg1	supplementation					20:34	High Dose Vitamin D supplementation	0:34	High Dose Vitamin D supplementation	0:34	High Dose Vitamin D supplementation alters faecal microbiome and predisposes mice to more severe colitis.
30065252	4	34	theme	DNA	757:759	arg1	analysis					738:745	Microbiota analysis	727:745	Microbiota analysis of faecal DNA	727:759	Microbiota analysis of faecal DNA showed that the microbial composition of D++ control mice was more similar to that of DSS mice.
30065252	6	35	theme	increased	1080:1088	arg1	susceptibility					1090:1103	increased susceptibility	1080:1103	increased susceptibility to colitis	1080:1114	Thus, high dose vitamin D supplementation is associated with a shift to a more inflammatory faecal microbiome and increased susceptibility to colitis, with a fall in circulating vitamin D occurring as a secondary event in response to the inflammatory process.
30065252	5	36	theme	Serum	857:861	arg1	levels					872:877	Serum 25(OH)D3 levels	857:877	Serum 25(OH)D3 levels	857:877	Serum 25(OH)D3 levels reduced by 63% in the D++ group and 23% in the D+ group after 6 days of DSS treatment.
30065252	6	37	theme	high	972:975	arg1	supplementation					992:1006	high dose vitamin D supplementation	972:1006	high dose vitamin D supplementation	972:1006	Thus, high dose vitamin D supplementation is associated with a shift to a more inflammatory faecal microbiome and increased susceptibility to colitis, with a fall in circulating vitamin D occurring as a secondary event in response to the inflammatory process.
30065252	1	38	theme	disease	184:190	arg1	severity					192:199	disease severity	184:199	disease severity in human inflammatory bowel disease	184:235	Vitamin D has been suggested as a possible adjunctive treatment to ameliorate disease severity in human inflammatory bowel disease.
30065252	0	39	theme	Vitamin	10:16	arg1	supplementation					20:34	High Dose Vitamin D supplementation	0:34	High Dose Vitamin D supplementation	0:34	High Dose Vitamin D supplementation alters faecal microbiome and predisposes mice to more severe colitis.
30065252	4	40	theme	faecal	750:755	arg1	DNA					757:759	faecal DNA	750:759	faecal DNA	750:759	Microbiota analysis of faecal DNA showed that the microbial composition of D++ control mice was more similar to that of DSS mice.
30065252	6	41	theme	vitamin	1144:1150	arg1	D					1152:1152	vitamin D	1144:1152	vitamin D occurring as a secondary event in response to the inflammatory process	1144:1223	Thus, high dose vitamin D supplementation is associated with a shift to a more inflammatory faecal microbiome and increased susceptibility to colitis, with a fall in circulating vitamin D occurring as a secondary event in response to the inflammatory process.
30065252	2	42	theme	sodium	386:391	arg1	colitis					408:414	dextran sodium sulphate (DSS) colitis	378:414	dextran sodium sulphate (DSS) colitis in female C57Bl/6 mice	378:437	In this study, the effects of diets containing high (D++, 10,000 IU/kg), moderate (D+, 2,280 IU/kg) or no vitamin D (D-) on the severity of dextran sodium sulphate (DSS) colitis in female C57Bl/6 mice were investigated.
30065252	5	43	from	%	892:892	arg1	group					929:933	the D+ group	922:933	the D+ group	922:933	Serum 25(OH)D3 levels reduced by 63% in the D++ group and 23% in the D+ group after 6 days of DSS treatment.
30065252	5	43	from	%	892:892	arg1	group					905:909	the D++ group	897:909	the D++ group	897:909	Serum 25(OH)D3 levels reduced by 63% in the D++ group and 23% in the D+ group after 6 days of DSS treatment.
30065252	5	44	theme	D3	869:870	arg1	levels					872:877	Serum 25(OH)D3 levels	857:877	Serum 25(OH)D3 levels	857:877	Serum 25(OH)D3 levels reduced by 63% in the D++ group and 23% in the D+ group after 6 days of DSS treatment.
30065252	6	45	theme	secondary	1169:1177	arg1	event					1179:1183	a secondary event	1167:1183	a secondary event in response to the inflammatory process	1167:1223	Thus, high dose vitamin D supplementation is associated with a shift to a more inflammatory faecal microbiome and increased susceptibility to colitis, with a fall in circulating vitamin D occurring as a secondary event in response to the inflammatory process.
30065252	2	46	theme	vitamin	344:350	arg1	D-					355:356	D-	355:356	D-	355:356	In this study, the effects of diets containing high (D++, 10,000 IU/kg), moderate (D+, 2,280 IU/kg) or no vitamin D (D-) on the severity of dextran sodium sulphate (DSS) colitis in female C57Bl/6 mice were investigated.
30065252	2	46	theme	vitamin	344:350	arg1	D					352:352	no vitamin D	341:352	no vitamin D (D-)	341:357	In this study, the effects of diets containing high (D++, 10,000 IU/kg), moderate (D+, 2,280 IU/kg) or no vitamin D (D-) on the severity of dextran sodium sulphate (DSS) colitis in female C57Bl/6 mice were investigated.
30065252	2	47	theme	dextran	378:384	arg1	colitis					408:414	dextran sodium sulphate (DSS) colitis	378:414	dextran sodium sulphate (DSS) colitis in female C57Bl/6 mice	378:437	In this study, the effects of diets containing high (D++, 10,000 IU/kg), moderate (D+, 2,280 IU/kg) or no vitamin D (D-) on the severity of dextran sodium sulphate (DSS) colitis in female C57Bl/6 mice were investigated.
30065252	2	48	contain	containing	274:283	arg2	D					352:352	no vitamin D	341:352	no vitamin D (D-)	341:357	In this study, the effects of diets containing high (D++, 10,000 IU/kg), moderate (D+, 2,280 IU/kg) or no vitamin D (D-) on the severity of dextran sodium sulphate (DSS) colitis in female C57Bl/6 mice were investigated.
30065252	2	48	contain	containing	274:283	arg2	high					285:288	high	285:288	high	285:288	In this study, the effects of diets containing high (D++, 10,000 IU/kg), moderate (D+, 2,280 IU/kg) or no vitamin D (D-) on the severity of dextran sodium sulphate (DSS) colitis in female C57Bl/6 mice were investigated.
30065252	2	48	contain	containing	274:283	arg1	diets					268:272	diets	268:272	diets containing high (D++, 10,000 IU/kg), moderate (D+, 2,280 IU/kg) or no vitamin D (D-)	268:357	In this study, the effects of diets containing high (D++, 10,000 IU/kg), moderate (D+, 2,280 IU/kg) or no vitamin D (D-) on the severity of dextran sodium sulphate (DSS) colitis in female C57Bl/6 mice were investigated.
30065252	2	48	contain	containing	274:283	arg2	moderate					311:318	moderate	311:318	moderate	311:318	In this study, the effects of diets containing high (D++, 10,000 IU/kg), moderate (D+, 2,280 IU/kg) or no vitamin D (D-) on the severity of dextran sodium sulphate (DSS) colitis in female C57Bl/6 mice were investigated.
30065252	2	48	contain	containing	274:283	arg2	D-					355:356	D-	355:356	D-	355:356	In this study, the effects of diets containing high (D++, 10,000 IU/kg), moderate (D+, 2,280 IU/kg) or no vitamin D (D-) on the severity of dextran sodium sulphate (DSS) colitis in female C57Bl/6 mice were investigated.
30065252	2	48	contain	containing	274:283	arg2	D+					321:322	D+	321:322	D+	321:322	In this study, the effects of diets containing high (D++, 10,000 IU/kg), moderate (D+, 2,280 IU/kg) or no vitamin D (D-) on the severity of dextran sodium sulphate (DSS) colitis in female C57Bl/6 mice were investigated.
30065252	3	49	from	p < 0.001	566:574	arg1	p = 0.05					663:670	p = 0.05	663:670	p = 0.05	663:670	The group on high dose vitamin D (D++) developed the most severe colitis as measured by blinded endoscopic (p < 0.001) and histologic (p < 0.05) assessment, weight loss (p < 0.001), drop in serum albumin (p = 0.05) and increased expression of colonic TNF-α (p < 0.05).
30065252	3	49	from	p < 0.001	566:574	arg1	albumin					654:660	serum albumin	648:660	serum albumin (p = 0.05)	648:671	The group on high dose vitamin D (D++) developed the most severe colitis as measured by blinded endoscopic (p < 0.001) and histologic (p < 0.05) assessment, weight loss (p < 0.001), drop in serum albumin (p = 0.05) and increased expression of colonic TNF-α (p < 0.05).
30065252	6	50	theme	faecal	1058:1063	arg1	microbiome					1065:1074	a more inflammatory faecal microbiome	1038:1074	a more inflammatory faecal microbiome	1038:1074	Thus, high dose vitamin D supplementation is associated with a shift to a more inflammatory faecal microbiome and increased susceptibility to colitis, with a fall in circulating vitamin D occurring as a secondary event in response to the inflammatory process.
30065252	6	51	dep	a	1122:1122	arg1	fall					1124:1127	fall	1124:1127	fall in circulating vitamin D occurring as a secondary event in response to the inflammatory process	1124:1223	Thus, high dose vitamin D supplementation is associated with a shift to a more inflammatory faecal microbiome and increased susceptibility to colitis, with a fall in circulating vitamin D occurring as a secondary event in response to the inflammatory process.
30065252	1	52	theme	human	204:208	arg1	disease					229:235	human inflammatory bowel disease	204:235	human inflammatory bowel disease	204:235	Vitamin D has been suggested as a possible adjunctive treatment to ameliorate disease severity in human inflammatory bowel disease.
30065252	2	53	theme	C57Bl/6	426:432	arg1	mice					434:437	female C57Bl/6 mice	419:437	female C57Bl/6 mice	419:437	In this study, the effects of diets containing high (D++, 10,000 IU/kg), moderate (D+, 2,280 IU/kg) or no vitamin D (D-) on the severity of dextran sodium sulphate (DSS) colitis in female C57Bl/6 mice were investigated.
30065252	3	54	from	drop	640:643	arg1	p = 0.05					663:670	p = 0.05	663:670	p = 0.05	663:670	The group on high dose vitamin D (D++) developed the most severe colitis as measured by blinded endoscopic (p < 0.001) and histologic (p < 0.05) assessment, weight loss (p < 0.001), drop in serum albumin (p = 0.05) and increased expression of colonic TNF-α (p < 0.05).
30065252	3	54	from	drop	640:643	arg1	albumin					654:660	serum albumin	648:660	serum albumin (p = 0.05)	648:671	The group on high dose vitamin D (D++) developed the most severe colitis as measured by blinded endoscopic (p < 0.001) and histologic (p < 0.05) assessment, weight loss (p < 0.001), drop in serum albumin (p = 0.05) and increased expression of colonic TNF-α (p < 0.05).
30065252	3	55	theme	severe	516:521	arg1	colitis					523:529	the most severe colitis	507:529	the most severe colitis	507:529	The group on high dose vitamin D (D++) developed the most severe colitis as measured by blinded endoscopic (p < 0.001) and histologic (p < 0.05) assessment, weight loss (p < 0.001), drop in serum albumin (p = 0.05) and increased expression of colonic TNF-α (p < 0.05).
30065252	3	56	theme	dose	476:479	arg1	D					489:489	high dose vitamin D	471:489	high dose vitamin D (D++)	471:495	The group on high dose vitamin D (D++) developed the most severe colitis as measured by blinded endoscopic (p < 0.001) and histologic (p < 0.05) assessment, weight loss (p < 0.001), drop in serum albumin (p = 0.05) and increased expression of colonic TNF-α (p < 0.05).
30065252	3	56	theme	dose	476:479	arg1	D++					492:494	D++	492:494	D++	492:494	The group on high dose vitamin D (D++) developed the most severe colitis as measured by blinded endoscopic (p < 0.001) and histologic (p < 0.05) assessment, weight loss (p < 0.001), drop in serum albumin (p = 0.05) and increased expression of colonic TNF-α (p < 0.05).
30065252	1	57	theme	Vitamin	106:112	arg1	D					114:114	Vitamin D	106:114	Vitamin D	106:114	Vitamin D has been suggested as a possible adjunctive treatment to ameliorate disease severity in human inflammatory bowel disease.
30065252	1	57	theme	Vitamin	106:112	arg1	treatment					160:168	a possible adjunctive treatment	138:168	a possible adjunctive treatment to ameliorate disease severity in human inflammatory bowel disease	138:235	Vitamin D has been suggested as a possible adjunctive treatment to ameliorate disease severity in human inflammatory bowel disease.
30065252	0	58	theme	faecal	43:48	arg1	microbiome					50:59	faecal microbiome	43:59	faecal microbiome	43:59	High Dose Vitamin D supplementation alters faecal microbiome and predisposes mice to more severe colitis.
30065252	3	59	theme	blinded	546:552	arg1	p < 0.001					566:574	blinded endoscopic (p < 0.001) and histologic (p < 0.05) assessment	546:612	p < 0.001	566:574	The group on high dose vitamin D (D++) developed the most severe colitis as measured by blinded endoscopic (p < 0.001) and histologic (p < 0.05) assessment, weight loss (p < 0.001), drop in serum albumin (p = 0.05) and increased expression of colonic TNF-α (p < 0.05).
30065252	3	60	theme	vitamin	481:487	arg1	D					489:489	high dose vitamin D	471:489	high dose vitamin D (D++)	471:495	The group on high dose vitamin D (D++) developed the most severe colitis as measured by blinded endoscopic (p < 0.001) and histologic (p < 0.05) assessment, weight loss (p < 0.001), drop in serum albumin (p = 0.05) and increased expression of colonic TNF-α (p < 0.05).
30065252	3	60	theme	vitamin	481:487	arg1	D++					492:494	D++	492:494	D++	492:494	The group on high dose vitamin D (D++) developed the most severe colitis as measured by blinded endoscopic (p < 0.001) and histologic (p < 0.05) assessment, weight loss (p < 0.001), drop in serum albumin (p = 0.05) and increased expression of colonic TNF-α (p < 0.05).
30065252	2	61	from	effects	257:263	arg1	severity					366:373	the severity	362:373	the severity of dextran sodium sulphate (DSS) colitis in female C57Bl/6 mice	362:437	In this study, the effects of diets containing high (D++, 10,000 IU/kg), moderate (D+, 2,280 IU/kg) or no vitamin D (D-) on the severity of dextran sodium sulphate (DSS) colitis in female C57Bl/6 mice were investigated.
30065252	2	62	from	colitis	408:414	arg1	mice					434:437	female C57Bl/6 mice	419:437	female C57Bl/6 mice	419:437	In this study, the effects of diets containing high (D++, 10,000 IU/kg), moderate (D+, 2,280 IU/kg) or no vitamin D (D-) on the severity of dextran sodium sulphate (DSS) colitis in female C57Bl/6 mice were investigated.
30065252	1	63	from	severity	192:199	arg1	disease					229:235	human inflammatory bowel disease	204:235	human inflammatory bowel disease	204:235	Vitamin D has been suggested as a possible adjunctive treatment to ameliorate disease severity in human inflammatory bowel disease.
30065252	6	64	theme	inflammatory	1045:1056	arg1	microbiome					1065:1074	a more inflammatory faecal microbiome	1038:1074	a more inflammatory faecal microbiome	1038:1074	Thus, high dose vitamin D supplementation is associated with a shift to a more inflammatory faecal microbiome and increased susceptibility to colitis, with a fall in circulating vitamin D occurring as a secondary event in response to the inflammatory process.
30065252	3	65	theme	colonic	701:707	arg1	p < 0.05					716:723	p < 0.05	716:723	p < 0.05	716:723	The group on high dose vitamin D (D++) developed the most severe colitis as measured by blinded endoscopic (p < 0.001) and histologic (p < 0.05) assessment, weight loss (p < 0.001), drop in serum albumin (p = 0.05) and increased expression of colonic TNF-α (p < 0.05).
30065252	3	65	theme	colonic	701:707	arg1	TNF-α					709:713	colonic TNF-α	701:713	colonic TNF-α (p < 0.05)	701:724	The group on high dose vitamin D (D++) developed the most severe colitis as measured by blinded endoscopic (p < 0.001) and histologic (p < 0.05) assessment, weight loss (p < 0.001), drop in serum albumin (p = 0.05) and increased expression of colonic TNF-α (p < 0.05).
30065252	1	66	theme	inflammatory	210:221	arg1	disease					229:235	human inflammatory bowel disease	204:235	human inflammatory bowel disease	204:235	Vitamin D has been suggested as a possible adjunctive treatment to ameliorate disease severity in human inflammatory bowel disease.
30065252	2	67	dep	D+	321:322	arg1	IU/kg					331:335	2,280 IU/kg	325:335	2,280 IU/kg	325:335	In this study, the effects of diets containing high (D++, 10,000 IU/kg), moderate (D+, 2,280 IU/kg) or no vitamin D (D-) on the severity of dextran sodium sulphate (DSS) colitis in female C57Bl/6 mice were investigated.
30065252	6	68	from	event	1179:1183	arg1	response					1188:1195	response	1188:1195	response to the inflammatory process	1188:1223	Thus, high dose vitamin D supplementation is associated with a shift to a more inflammatory faecal microbiome and increased susceptibility to colitis, with a fall in circulating vitamin D occurring as a secondary event in response to the inflammatory process.
30065252	3	69	theme	increased	677:685	arg1	expression					687:696	increased expression	677:696	increased expression of colonic TNF-α (p < 0.05)	677:724	The group on high dose vitamin D (D++) developed the most severe colitis as measured by blinded endoscopic (p < 0.001) and histologic (p < 0.05) assessment, weight loss (p < 0.001), drop in serum albumin (p = 0.05) and increased expression of colonic TNF-α (p < 0.05).
30065252	1	70	theme	bowel	223:227	arg1	disease					229:235	human inflammatory bowel disease	204:235	human inflammatory bowel disease	204:235	Vitamin D has been suggested as a possible adjunctive treatment to ameliorate disease severity in human inflammatory bowel disease.
30065252	4	71	theme	control	806:812	arg1	mice					814:817	D++ control mice	802:817	D++ control mice	802:817	Microbiota analysis of faecal DNA showed that the microbial composition of D++ control mice was more similar to that of DSS mice.
30065252	2	72	dep	high	285:288	arg1	10,000 IU/kg					296:307	10,000 IU/kg	296:307	10,000 IU/kg	296:307	In this study, the effects of diets containing high (D++, 10,000 IU/kg), moderate (D+, 2,280 IU/kg) or no vitamin D (D-) on the severity of dextran sodium sulphate (DSS) colitis in female C57Bl/6 mice were investigated.
30065252	2	72	dep	high	285:288	arg1	D++					291:293	D++	291:293	D++	291:293	In this study, the effects of diets containing high (D++, 10,000 IU/kg), moderate (D+, 2,280 IU/kg) or no vitamin D (D-) on the severity of dextran sodium sulphate (DSS) colitis in female C57Bl/6 mice were investigated.
30065252	3	73	from	group	462:466	arg1	D					489:489	high dose vitamin D	471:489	high dose vitamin D (D++)	471:495	The group on high dose vitamin D (D++) developed the most severe colitis as measured by blinded endoscopic (p < 0.001) and histologic (p < 0.05) assessment, weight loss (p < 0.001), drop in serum albumin (p = 0.05) and increased expression of colonic TNF-α (p < 0.05).
30065252	3	73	from	group	462:466	arg1	D++					492:494	D++	492:494	D++	492:494	The group on high dose vitamin D (D++) developed the most severe colitis as measured by blinded endoscopic (p < 0.001) and histologic (p < 0.05) assessment, weight loss (p < 0.001), drop in serum albumin (p = 0.05) and increased expression of colonic TNF-α (p < 0.05).
30065252	2	74	theme	DSS	403:405	arg1	colitis					408:414	dextran sodium sulphate (DSS) colitis	378:414	dextran sodium sulphate (DSS) colitis in female C57Bl/6 mice	378:437	In this study, the effects of diets containing high (D++, 10,000 IU/kg), moderate (D+, 2,280 IU/kg) or no vitamin D (D-) on the severity of dextran sodium sulphate (DSS) colitis in female C57Bl/6 mice were investigated.
30065252	6	75	theme	dose	977:980	arg1	supplementation					992:1006	high dose vitamin D supplementation	972:1006	high dose vitamin D supplementation	972:1006	Thus, high dose vitamin D supplementation is associated with a shift to a more inflammatory faecal microbiome and increased susceptibility to colitis, with a fall in circulating vitamin D occurring as a secondary event in response to the inflammatory process.
30065252	3	76	theme	serum	648:652	arg1	p = 0.05					663:670	p = 0.05	663:670	p = 0.05	663:670	The group on high dose vitamin D (D++) developed the most severe colitis as measured by blinded endoscopic (p < 0.001) and histologic (p < 0.05) assessment, weight loss (p < 0.001), drop in serum albumin (p = 0.05) and increased expression of colonic TNF-α (p < 0.05).
30065252	3	76	theme	serum	648:652	arg1	albumin					654:660	serum albumin	648:660	serum albumin (p = 0.05)	648:671	The group on high dose vitamin D (D++) developed the most severe colitis as measured by blinded endoscopic (p < 0.001) and histologic (p < 0.05) assessment, weight loss (p < 0.001), drop in serum albumin (p = 0.05) and increased expression of colonic TNF-α (p < 0.05).
31859155	5	0	theme	substrate	745:753	arg1	synthesis					704:712	synthesis	704:712	synthesis	704:712	Herein we describe the synthesis and enzymatic study of a model substrate involving 4-nitroaniline as an amine-containing chromogenic reporter.
31859155	5	0	theme	substrate	745:753	arg1	study					728:732	enzymatic study	718:732	enzymatic study	718:732	Herein we describe the synthesis and enzymatic study of a model substrate involving 4-nitroaniline as an amine-containing chromogenic reporter.
31859155	1	1	theme	pathological	191:202	arg1	roles					204:208	the physiological and pathological roles	169:208	the physiological and pathological roles of neuraminidases	169:226	Artificial substrates for probing neuraminidase activity are powerful tools for studying the physiological and pathological roles of neuraminidases.
31859155	4	2	theme	amine-containing	618:633	arg1	compounds					635:643	amine-containing compounds	618:643	amine-containing compounds	618:643	To expand the applicability of reporters to amine-containing compounds, we have focused on prodrug design.
31859155	8	3	theme	intermediate	1164:1175	arg1	methide					1156:1162	quinone methide intermediate	1148:1175	quinone methide intermediate	1148:1175	The elimination process involves generation of quinone methide intermediate, which causes to neutralize neuraminidase.
31859155	8	4	theme	methide	1156:1162	arg1	generation					1134:1143	generation	1134:1143	generation	1134:1143	The elimination process involves generation of quinone methide intermediate, which causes to neutralize neuraminidase.
31859155	1	5	theme	Artificial	80:89	arg1	tools					150:154	powerful tools	141:154	powerful tools for studying the physiological and pathological roles of neuraminidases	141:226	Artificial substrates for probing neuraminidase activity are powerful tools for studying the physiological and pathological roles of neuraminidases.
31859155	1	5	theme	Artificial	80:89	arg1	substrates					91:100	Artificial substrates	80:100	Artificial substrates for probing neuraminidase activity	80:135	Artificial substrates for probing neuraminidase activity are powerful tools for studying the physiological and pathological roles of neuraminidases.
31859155	2	6	theme	residues	393:400	arg1	cleavage					365:372	neuraminidase-catalyzed cleavage	341:372	neuraminidase-catalyzed cleavage of the sialic acid residues	341:400	Most of the substrates are α-O-linked sialosides involving hydroxyl-containing reporters for visualization, and neuraminidase-catalyzed cleavage of the sialic acid residues directly activates the reporters.
31859155	7	7	theme	linker	1020:1025	arg1	moiety					1027:1032	the linker moiety	1016:1032	the linker moiety	1016:1032	Neuraminidase-mediated hydrolysis of the sialic acid moiety of the substrate initiates self-immolative elimination of the linker moiety, leading the liberation of yellow-colored reporter 4-nitroaniline.
31859155	3	8	link	α-N-linked	508:517	arg1	sialosides					519:528	α-N-linked sialosides	508:528	α-N-linked sialosides	508:528	However, the use of amine-containing reporters has been avoided because α-N-linked sialosides are marginal substrates for neuraminidases.
31859155	3	8	link	α-N-linked	508:517	arg1	substrates					543:552	marginal substrates	534:552	marginal substrates for neuraminidases	534:571	However, the use of amine-containing reporters has been avoided because α-N-linked sialosides are marginal substrates for neuraminidases.
31859155	5	9	theme	enzymatic	718:726	arg1	study					728:732	enzymatic study	718:732	enzymatic study	718:732	Herein we describe the synthesis and enzymatic study of a model substrate involving 4-nitroaniline as an amine-containing chromogenic reporter.
31859155	2	10	theme	neuraminidase-catalyzed	341:363	arg1	cleavage					365:372	neuraminidase-catalyzed cleavage	341:372	neuraminidase-catalyzed cleavage of the sialic acid residues	341:400	Most of the substrates are α-O-linked sialosides involving hydroxyl-containing reporters for visualization, and neuraminidase-catalyzed cleavage of the sialic acid residues directly activates the reporters.
31859155	7	11	theme	moiety	1027:1032	arg1	elimination					1001:1011	self-immolative elimination	985:1011	self-immolative elimination of the linker moiety	985:1032	Neuraminidase-mediated hydrolysis of the sialic acid moiety of the substrate initiates self-immolative elimination of the linker moiety, leading the liberation of yellow-colored reporter 4-nitroaniline.
31859155	0	12	theme	Neuraminidase-triggered	0:22	arg1	activation					24:33	Neuraminidase-triggered activation	0:33	Neuraminidase-triggered activation of prodrug-type substrate of 4-nitroaniline.	0:78	Neuraminidase-triggered activation of prodrug-type substrate of 4-nitroaniline.
31859155	1	13	theme	neuraminidases	213:226	arg1	roles					204:208	the physiological and pathological roles	169:208	the physiological and pathological roles of neuraminidases	169:226	Artificial substrates for probing neuraminidase activity are powerful tools for studying the physiological and pathological roles of neuraminidases.
31859155	3	14	theme	amine-containing	456:471	arg1	reporters					473:481	amine-containing reporters	456:481	amine-containing reporters	456:481	However, the use of amine-containing reporters has been avoided because α-N-linked sialosides are marginal substrates for neuraminidases.
31859155	7	15	theme	moiety	951:956	arg1	hydrolysis					921:930	Neuraminidase-mediated hydrolysis	898:930	Neuraminidase-mediated hydrolysis of the sialic acid moiety of the substrate	898:973	Neuraminidase-mediated hydrolysis of the sialic acid moiety of the substrate initiates self-immolative elimination of the linker moiety, leading the liberation of yellow-colored reporter 4-nitroaniline.
31859155	1	16	theme	neuraminidase	114:126	arg1	activity					128:135	neuraminidase activity	114:135	neuraminidase activity	114:135	Artificial substrates for probing neuraminidase activity are powerful tools for studying the physiological and pathological roles of neuraminidases.
31859155	9	17	theme	chromogenic	1261:1271	arg1	substrate					1273:1281	a chromogenic substrate	1259:1281	not only a chromogenic substrate but also a suicide inactivator	1250:1312	The substrate, thus, works as not only a chromogenic substrate but also a suicide inactivator.
31859155	3	18	theme	α-N-linked	508:517	arg1	sialosides					519:528	α-N-linked sialosides	508:528	α-N-linked sialosides	508:528	However, the use of amine-containing reporters has been avoided because α-N-linked sialosides are marginal substrates for neuraminidases.
31859155	3	18	theme	α-N-linked	508:517	arg1	substrates					543:552	marginal substrates	534:552	marginal substrates for neuraminidases	534:571	However, the use of amine-containing reporters has been avoided because α-N-linked sialosides are marginal substrates for neuraminidases.
31859155	3	19	theme	reporters	473:481	arg1	use					449:451	the use	445:451	the use of amine-containing reporters	445:481	However, the use of amine-containing reporters has been avoided because α-N-linked sialosides are marginal substrates for neuraminidases.
31859155	5	20	theme	model	739:743	arg1	substrate					745:753	a model substrate	737:753	a model substrate involving 4-nitroaniline as an amine-containing chromogenic reporter	737:822	Herein we describe the synthesis and enzymatic study of a model substrate involving 4-nitroaniline as an amine-containing chromogenic reporter.
31859155	2	21	theme	acid	388:391	arg1	residues					393:400	the sialic acid residues	377:400	the sialic acid residues	377:400	Most of the substrates are α-O-linked sialosides involving hydroxyl-containing reporters for visualization, and neuraminidase-catalyzed cleavage of the sialic acid residues directly activates the reporters.
31859155	7	22	theme	self-immolative	985:999	arg1	elimination					1001:1011	self-immolative elimination	985:1011	self-immolative elimination of the linker moiety	985:1032	Neuraminidase-mediated hydrolysis of the sialic acid moiety of the substrate initiates self-immolative elimination of the linker moiety, leading the liberation of yellow-colored reporter 4-nitroaniline.
31859155	0	23	theme	prodrug-type	38:49	arg1	substrate					51:59	prodrug-type substrate	38:59	prodrug-type substrate of 4-nitroaniline	38:77	Neuraminidase-triggered activation of prodrug-type substrate of 4-nitroaniline.
31859155	2	24	theme	sialic	381:386	arg1	residues					393:400	the sialic acid residues	377:400	the sialic acid residues	377:400	Most of the substrates are α-O-linked sialosides involving hydroxyl-containing reporters for visualization, and neuraminidase-catalyzed cleavage of the sialic acid residues directly activates the reporters.
31859155	7	25	theme	acid	946:949	arg1	moiety					951:956	the sialic acid moiety	935:956	the sialic acid moiety of the substrate	935:973	Neuraminidase-mediated hydrolysis of the sialic acid moiety of the substrate initiates self-immolative elimination of the linker moiety, leading the liberation of yellow-colored reporter 4-nitroaniline.
31859155	1	26	theme	powerful	141:148	arg1	tools					150:154	powerful tools	141:154	powerful tools for studying the physiological and pathological roles of neuraminidases	141:226	Artificial substrates for probing neuraminidase activity are powerful tools for studying the physiological and pathological roles of neuraminidases.
31859155	1	26	theme	powerful	141:148	arg1	substrates					91:100	Artificial substrates	80:100	Artificial substrates for probing neuraminidase activity	80:135	Artificial substrates for probing neuraminidase activity are powerful tools for studying the physiological and pathological roles of neuraminidases.
31859155	7	27	theme	substrate	965:973	arg1	moiety					951:956	the sialic acid moiety	935:956	the sialic acid moiety of the substrate	935:973	Neuraminidase-mediated hydrolysis of the sialic acid moiety of the substrate initiates self-immolative elimination of the linker moiety, leading the liberation of yellow-colored reporter 4-nitroaniline.
31859155	4	28	theme	prodrug	665:671	arg1	design					673:678	prodrug design	665:678	prodrug design	665:678	To expand the applicability of reporters to amine-containing compounds, we have focused on prodrug design.
31859155	2	29	theme	hydroxyl-containing	288:306	arg1	reporters					308:316	hydroxyl-containing reporters	288:316	hydroxyl-containing reporters	288:316	Most of the substrates are α-O-linked sialosides involving hydroxyl-containing reporters for visualization, and neuraminidase-catalyzed cleavage of the sialic acid residues directly activates the reporters.
31859155	5	30	dep	synthesis	704:712	arg1	the					700:702	the	700:702	the	700:702	Herein we describe the synthesis and enzymatic study of a model substrate involving 4-nitroaniline as an amine-containing chromogenic reporter.
31859155	0	31	theme	substrate	51:59	arg1	activation					24:33	Neuraminidase-triggered activation	0:33	Neuraminidase-triggered activation of prodrug-type substrate of 4-nitroaniline.	0:78	Neuraminidase-triggered activation of prodrug-type substrate of 4-nitroaniline.
31859155	7	32	theme	yellow-colored	1061:1074	arg1	4-nitroaniline					1085:1098	yellow-colored reporter 4-nitroaniline	1061:1098	yellow-colored reporter 4-nitroaniline	1061:1098	Neuraminidase-mediated hydrolysis of the sialic acid moiety of the substrate initiates self-immolative elimination of the linker moiety, leading the liberation of yellow-colored reporter 4-nitroaniline.
31859155	9	33	theme	suicide	1294:1300	arg1	inactivator					1302:1312	a suicide inactivator	1292:1312	not only a chromogenic substrate but also a suicide inactivator	1250:1312	The substrate, thus, works as not only a chromogenic substrate but also a suicide inactivator.
31859155	7	34	theme	sialic	939:944	arg1	moiety					951:956	the sialic acid moiety	935:956	the sialic acid moiety of the substrate	935:973	Neuraminidase-mediated hydrolysis of the sialic acid moiety of the substrate initiates self-immolative elimination of the linker moiety, leading the liberation of yellow-colored reporter 4-nitroaniline.
31859155	6	35	from	perfringens	885:895	arg1	neuraminidase					854:866	neuraminidase	854:866	neuraminidase from Clostridium perfringens	854:895	The substrate can respond to neuraminidase from Clostridium perfringens.
31859155	0	36	theme	4-nitroaniline	64:77	arg1	substrate					51:59	prodrug-type substrate	38:59	prodrug-type substrate of 4-nitroaniline	38:77	Neuraminidase-triggered activation of prodrug-type substrate of 4-nitroaniline.
31859155	5	37	theme	amine-containing	786:801	arg1	reporter					815:822	an amine-containing chromogenic reporter	783:822	an amine-containing chromogenic reporter	783:822	Herein we describe the synthesis and enzymatic study of a model substrate involving 4-nitroaniline as an amine-containing chromogenic reporter.
31859155	5	37	theme	amine-containing	786:801	arg1	4-nitroaniline					765:778	4-nitroaniline	765:778	4-nitroaniline	765:778	Herein we describe the synthesis and enzymatic study of a model substrate involving 4-nitroaniline as an amine-containing chromogenic reporter.
31859155	2	38	theme	α-O-linked	256:265	arg1	Most					229:232	Most	229:232	Most	229:232	Most of the substrates are α-O-linked sialosides involving hydroxyl-containing reporters for visualization, and neuraminidase-catalyzed cleavage of the sialic acid residues directly activates the reporters.
31859155	2	38	theme	α-O-linked	256:265	arg1	substrates					241:250	the substrates	237:250	the substrates	237:250	Most of the substrates are α-O-linked sialosides involving hydroxyl-containing reporters for visualization, and neuraminidase-catalyzed cleavage of the sialic acid residues directly activates the reporters.
31859155	2	38	theme	α-O-linked	256:265	arg1	sialosides					267:276	α-O-linked sialosides	256:276	α-O-linked sialosides involving hydroxyl-containing reporters for visualization	256:334	Most of the substrates are α-O-linked sialosides involving hydroxyl-containing reporters for visualization, and neuraminidase-catalyzed cleavage of the sialic acid residues directly activates the reporters.
31859155	7	39	theme	reporter	1076:1083	arg1	4-nitroaniline					1085:1098	yellow-colored reporter 4-nitroaniline	1061:1098	yellow-colored reporter 4-nitroaniline	1061:1098	Neuraminidase-mediated hydrolysis of the sialic acid moiety of the substrate initiates self-immolative elimination of the linker moiety, leading the liberation of yellow-colored reporter 4-nitroaniline.
31859155	5	40	theme	chromogenic	803:813	arg1	reporter					815:822	an amine-containing chromogenic reporter	783:822	an amine-containing chromogenic reporter	783:822	Herein we describe the synthesis and enzymatic study of a model substrate involving 4-nitroaniline as an amine-containing chromogenic reporter.
31859155	5	40	theme	chromogenic	803:813	arg1	4-nitroaniline					765:778	4-nitroaniline	765:778	4-nitroaniline	765:778	Herein we describe the synthesis and enzymatic study of a model substrate involving 4-nitroaniline as an amine-containing chromogenic reporter.
31859155	6	41	theme	Clostridium	873:883	arg1	perfringens					885:895	Clostridium perfringens	873:895	Clostridium perfringens	873:895	The substrate can respond to neuraminidase from Clostridium perfringens.
31859155	7	42	theme	4-nitroaniline	1085:1098	arg1	liberation					1047:1056	the liberation	1043:1056	the liberation of yellow-colored reporter 4-nitroaniline	1043:1098	Neuraminidase-mediated hydrolysis of the sialic acid moiety of the substrate initiates self-immolative elimination of the linker moiety, leading the liberation of yellow-colored reporter 4-nitroaniline.
31859155	8	43	theme	elimination	1105:1115	arg1	process					1117:1123	The elimination process	1101:1123	The elimination process	1101:1123	The elimination process involves generation of quinone methide intermediate, which causes to neutralize neuraminidase.
31859155	2	44	link	α-O-linked	256:265	arg1	Most					229:232	Most	229:232	Most	229:232	Most of the substrates are α-O-linked sialosides involving hydroxyl-containing reporters for visualization, and neuraminidase-catalyzed cleavage of the sialic acid residues directly activates the reporters.
31859155	2	44	link	α-O-linked	256:265	arg1	substrates					241:250	the substrates	237:250	the substrates	237:250	Most of the substrates are α-O-linked sialosides involving hydroxyl-containing reporters for visualization, and neuraminidase-catalyzed cleavage of the sialic acid residues directly activates the reporters.
31859155	2	44	link	α-O-linked	256:265	arg1	sialosides					267:276	α-O-linked sialosides	256:276	α-O-linked sialosides involving hydroxyl-containing reporters for visualization	256:334	Most of the substrates are α-O-linked sialosides involving hydroxyl-containing reporters for visualization, and neuraminidase-catalyzed cleavage of the sialic acid residues directly activates the reporters.
31859155	7	45	theme	Neuraminidase-mediated	898:919	arg1	hydrolysis					921:930	Neuraminidase-mediated hydrolysis	898:930	Neuraminidase-mediated hydrolysis of the sialic acid moiety of the substrate	898:973	Neuraminidase-mediated hydrolysis of the sialic acid moiety of the substrate initiates self-immolative elimination of the linker moiety, leading the liberation of yellow-colored reporter 4-nitroaniline.
31859155	1	46	theme	physiological	173:185	arg1	roles					204:208	the physiological and pathological roles	169:208	the physiological and pathological roles of neuraminidases	169:226	Artificial substrates for probing neuraminidase activity are powerful tools for studying the physiological and pathological roles of neuraminidases.
31859155	3	47	theme	marginal	534:541	arg1	sialosides					519:528	α-N-linked sialosides	508:528	α-N-linked sialosides	508:528	However, the use of amine-containing reporters has been avoided because α-N-linked sialosides are marginal substrates for neuraminidases.
31859155	3	47	theme	marginal	534:541	arg1	substrates					543:552	marginal substrates	534:552	marginal substrates for neuraminidases	534:571	However, the use of amine-containing reporters has been avoided because α-N-linked sialosides are marginal substrates for neuraminidases.
31859155	4	48	theme	reporters	605:613	arg1	applicability					588:600	the applicability	584:600	the applicability of reporters to amine-containing compounds	584:643	To expand the applicability of reporters to amine-containing compounds, we have focused on prodrug design.
31859155	8	49	theme	quinone	1148:1154	arg1	methide					1156:1162	quinone methide intermediate	1148:1175	quinone methide intermediate	1148:1175	The elimination process involves generation of quinone methide intermediate, which causes to neutralize neuraminidase.
31336403	0	0	theme	Marked	82:87	arg1	Alterations					89:99	Marked Alterations	82:99	Marked Alterations of Gut Microbiome in Rats	82:125	High-Fat Diet Enriched with Bilberry Modifies Colonic Mucus Dynamics and Restores Marked Alterations of Gut Microbiome in Rats.
31336403	4	1	theme	mucus	481:485	arg1	thickness					487:495	colonic mucus thickness	473:495	colonic mucus thickness in vivo	473:503	METHODS AND RESULTS The effects on colonic mucus thickness in vivo and gut microbiota composition (Illumina sequencing, quantitative real-time PCR) are investigated in young rats fed a low-fat diet or HFD with or without bilberries for 8 weeks (n = 8).
31336403	5	2	theme	significant	703:713	arg1	effects					729:735	significant local colonic effects	703:735	significant local colonic effects	703:735	HFD induced significant local colonic effects, despite no observed weight gain or systemic inflammation, as HFD causes epithelial upregulation of inducible nitric oxide synthase, which is counteracted by bilberry.
31336403	5	3	theme	inducible	837:845	arg1	synthase					860:867	inducible nitric oxide synthase	837:867	inducible nitric oxide synthase	837:867	HFD induced significant local colonic effects, despite no observed weight gain or systemic inflammation, as HFD causes epithelial upregulation of inducible nitric oxide synthase, which is counteracted by bilberry.
31336403	1	4	theme	related	234:240	arg1	disorders					242:250	related disorders	234:250	related disorders	234:250	SCOPE Emerging evidence suggests that high-fat diet (HFD) is associated with gut microbiome dysbiosis and related disorders.
31336403	5	5	theme	local	715:719	arg1	effects					729:735	significant local colonic effects	703:735	significant local colonic effects	703:735	HFD induced significant local colonic effects, despite no observed weight gain or systemic inflammation, as HFD causes epithelial upregulation of inducible nitric oxide synthase, which is counteracted by bilberry.
31336403	5	6	theme	nitric	847:852	arg1	synthase					860:867	inducible nitric oxide synthase	837:867	inducible nitric oxide synthase	837:867	HFD induced significant local colonic effects, despite no observed weight gain or systemic inflammation, as HFD causes epithelial upregulation of inducible nitric oxide synthase, which is counteracted by bilberry.
31336403	2	7	with	component	282:290	arg1	benefits					310:317	known health benefits	297:317	known health benefits	297:317	Bilberry is a prebiotic food component with known health benefits.
31336403	9	8	dep	CONCLUSION	1290:1299	arg1	HFD					1301:1303	HFD	1301:1303	HFD	1301:1303	CONCLUSION HFD expand firmly adherent mucus thickness and reduce mucus-foraging bacteria populations in the colon prior to obesity.
31336403	2	9	theme	food	277:280	arg1	component					282:290	a prebiotic food component	265:290	a prebiotic food component with known health benefits	265:317	Bilberry is a prebiotic food component with known health benefits.
31336403	2	9	theme	food	277:280	arg1	Bilberry					253:260	Bilberry	253:260	Bilberry	253:260	Bilberry is a prebiotic food component with known health benefits.
31336403	4	10	dep	METHODS	438:444	arg1	investigated					590:601	investigated	590:601	are investigated in young rats fed a low-fat diet or HFD with or without bilberries for 8 weeks (n = 8)	586:688	METHODS AND RESULTS The effects on colonic mucus thickness in vivo and gut microbiota composition (Illumina sequencing, quantitative real-time PCR) are investigated in young rats fed a low-fat diet or HFD with or without bilberries for 8 weeks (n = 8).
31336403	2	11	theme	prebiotic	267:275	arg1	component					282:290	a prebiotic food component	265:290	a prebiotic food component with known health benefits	265:317	Bilberry is a prebiotic food component with known health benefits.
31336403	2	11	theme	prebiotic	267:275	arg1	Bilberry					253:260	Bilberry	253:260	Bilberry	253:260	Bilberry is a prebiotic food component with known health benefits.
31336403	6	12	theme	mucus	925:929	arg1	layer					931:935	The firmly adherent mucus layer	905:935	The firmly adherent mucus layer	905:935	The firmly adherent mucus layer becomes thicker and the mRNA levels of Muc2 and Tff3 are increased by HFD with or without bilberry.
31336403	0	13	theme	Microbiome	108:117	arg1	Alterations					89:99	Marked Alterations	82:99	Marked Alterations of Gut Microbiome in Rats	82:125	High-Fat Diet Enriched with Bilberry Modifies Colonic Mucus Dynamics and Restores Marked Alterations of Gut Microbiome in Rats.
31336403	9	14	theme	mucus-foraging	1355:1368	arg1	populations					1379:1389	mucus-foraging bacteria populations	1355:1389	mucus-foraging bacteria populations in the colon prior to obesity	1355:1419	CONCLUSION HFD expand firmly adherent mucus thickness and reduce mucus-foraging bacteria populations in the colon prior to obesity.
31336403	8	15	theme	microbiota	1199:1208	arg1	dysbiosis					1210:1218	HFD-induced microbiota dysbiosis	1187:1218	HFD-induced microbiota dysbiosis	1187:1218	Finally, bilberry prevents HFD-induced microbiota dysbiosis, including expansion of pathobionts, for example, Enterobacteriaceae.
31336403	8	16	theme	HFD-induced	1187:1197	arg1	dysbiosis					1210:1218	HFD-induced microbiota dysbiosis	1187:1218	HFD-induced microbiota dysbiosis	1187:1218	Finally, bilberry prevents HFD-induced microbiota dysbiosis, including expansion of pathobionts, for example, Enterobacteriaceae.
31336403	1	17	theme	high-fat	166:173	arg1	HFD					181:183	HFD	181:183	HFD	181:183	SCOPE Emerging evidence suggests that high-fat diet (HFD) is associated with gut microbiome dysbiosis and related disorders.
31336403	1	17	theme	high-fat	166:173	arg1	diet					175:178	high-fat diet	166:178	high-fat diet (HFD)	166:184	SCOPE Emerging evidence suggests that high-fat diet (HFD) is associated with gut microbiome dysbiosis and related disorders.
31336403	0	18	theme	Gut	104:106	arg1	Microbiome					108:117	Gut Microbiome	104:117	Gut Microbiome in Rats	104:125	High-Fat Diet Enriched with Bilberry Modifies Colonic Mucus Dynamics and Restores Marked Alterations of Gut Microbiome in Rats.
31336403	4	19	theme	colonic	473:479	arg1	thickness					487:495	colonic mucus thickness	473:495	colonic mucus thickness in vivo	473:503	METHODS AND RESULTS The effects on colonic mucus thickness in vivo and gut microbiota composition (Illumina sequencing, quantitative real-time PCR) are investigated in young rats fed a low-fat diet or HFD with or without bilberries for 8 weeks (n = 8).
31336403	5	20	theme	observed	749:756	arg1	gain					765:768	no observed weight gain	746:768	no observed weight gain	746:768	HFD induced significant local colonic effects, despite no observed weight gain or systemic inflammation, as HFD causes epithelial upregulation of inducible nitric oxide synthase, which is counteracted by bilberry.
31336403	7	21	theme	species	1107:1113	arg1	abundance					1074:1082	the colonic abundance	1062:1082	the colonic abundance of mucolytic bacterial species	1062:1113	In parallel, HFD reduced the colonic abundance of mucolytic bacterial species Akkermansia muciniphila and Bacteroides spp.
31336403	3	22	theme	layer	362:366	arg1	dynamics					332:339	the dynamics	328:339	the dynamics of the colonic mucus layer and microbiome during HFD and bilberry supplementation	328:421	Herein, the dynamics of the colonic mucus layer and microbiome during HFD and bilberry supplementation are addressed.
31336403	4	23	from	effects	462:468	arg1	thickness					487:495	colonic mucus thickness	473:495	colonic mucus thickness in vivo	473:503	METHODS AND RESULTS The effects on colonic mucus thickness in vivo and gut microbiota composition (Illumina sequencing, quantitative real-time PCR) are investigated in young rats fed a low-fat diet or HFD with or without bilberries for 8 weeks (n = 8).
31336403	4	23	from	effects	462:468	arg1	composition					524:534	gut microbiota composition	509:534	gut microbiota composition	509:534	METHODS AND RESULTS The effects on colonic mucus thickness in vivo and gut microbiota composition (Illumina sequencing, quantitative real-time PCR) are investigated in young rats fed a low-fat diet or HFD with or without bilberries for 8 weeks (n = 8).
31336403	6	24	theme	adherent	916:923	arg1	layer					931:935	The firmly adherent mucus layer	905:935	The firmly adherent mucus layer	905:935	The firmly adherent mucus layer becomes thicker and the mRNA levels of Muc2 and Tff3 are increased by HFD with or without bilberry.
31336403	2	25	theme	health	303:308	arg1	benefits					310:317	known health benefits	297:317	known health benefits	297:317	Bilberry is a prebiotic food component with known health benefits.
31336403	3	26	theme	bilberry	398:405	arg1	supplementation					407:421	bilberry supplementation	398:421	bilberry supplementation	398:421	Herein, the dynamics of the colonic mucus layer and microbiome during HFD and bilberry supplementation are addressed.
31336403	0	27	theme	High-Fat	0:7	arg1	Diet					9:12	High-Fat Diet	0:12	High-Fat Diet Enriched with Bilberry	0:35	High-Fat Diet Enriched with Bilberry Modifies Colonic Mucus Dynamics and Restores Marked Alterations of Gut Microbiome in Rats.
31336403	4	28	theme	Illumina	537:544	arg1	PCR					581:583	quantitative real-time PCR	558:583	quantitative real-time PCR	558:583	METHODS AND RESULTS The effects on colonic mucus thickness in vivo and gut microbiota composition (Illumina sequencing, quantitative real-time PCR) are investigated in young rats fed a low-fat diet or HFD with or without bilberries for 8 weeks (n = 8).
31336403	4	28	theme	Illumina	537:544	arg1	sequencing					546:555	Illumina sequencing	537:555	Illumina sequencing	537:555	METHODS AND RESULTS The effects on colonic mucus thickness in vivo and gut microbiota composition (Illumina sequencing, quantitative real-time PCR) are investigated in young rats fed a low-fat diet or HFD with or without bilberries for 8 weeks (n = 8).
31336403	9	29	theme	mucus	1328:1332	arg1	thickness					1334:1342	firmly adherent mucus thickness	1312:1342	firmly adherent mucus thickness	1312:1342	CONCLUSION HFD expand firmly adherent mucus thickness and reduce mucus-foraging bacteria populations in the colon prior to obesity.
31336403	2	30	theme	known	297:301	arg1	benefits					310:317	known health benefits	297:317	known health benefits	297:317	Bilberry is a prebiotic food component with known health benefits.
31336403	5	31	theme	epithelial	810:819	arg1	upregulation					821:832	epithelial upregulation	810:832	epithelial upregulation of inducible nitric oxide synthase, which is counteracted by bilberry	810:902	HFD induced significant local colonic effects, despite no observed weight gain or systemic inflammation, as HFD causes epithelial upregulation of inducible nitric oxide synthase, which is counteracted by bilberry.
31336403	3	32	theme	colonic	348:354	arg1	layer					362:366	the colonic mucus layer	344:366	the colonic mucus layer	344:366	Herein, the dynamics of the colonic mucus layer and microbiome during HFD and bilberry supplementation are addressed.
31336403	4	33	theme	=	685:685	arg1	weeks					676:680	8 weeks	674:680	8 weeks (n = 8)	674:688	METHODS AND RESULTS The effects on colonic mucus thickness in vivo and gut microbiota composition (Illumina sequencing, quantitative real-time PCR) are investigated in young rats fed a low-fat diet or HFD with or without bilberries for 8 weeks (n = 8).
31336403	4	33	theme	=	685:685	arg1	n					683:683	n = 8	683:687	n = 8	683:687	METHODS AND RESULTS The effects on colonic mucus thickness in vivo and gut microbiota composition (Illumina sequencing, quantitative real-time PCR) are investigated in young rats fed a low-fat diet or HFD with or without bilberries for 8 weeks (n = 8).
31336403	8	34	theme	pathobionts	1244:1254	arg1	expansion					1231:1239	expansion	1231:1239	expansion of pathobionts	1231:1254	Finally, bilberry prevents HFD-induced microbiota dysbiosis, including expansion of pathobionts, for example, Enterobacteriaceae.
31336403	6	35	theme	Tff3	985:988	arg1	levels					966:971	the mRNA levels	957:971	the mRNA levels of Muc2 and Tff3	957:988	The firmly adherent mucus layer becomes thicker and the mRNA levels of Muc2 and Tff3 are increased by HFD with or without bilberry.
31336403	3	36	theme	mucus	356:360	arg1	layer					362:366	the colonic mucus layer	344:366	the colonic mucus layer	344:366	Herein, the dynamics of the colonic mucus layer and microbiome during HFD and bilberry supplementation are addressed.
31336403	10	37	theme	intestinal	1467:1476	arg1	inflammation					1478:1489	intestinal inflammation	1467:1489	intestinal inflammation	1467:1489	Enriching HFD with bilberry protects against intestinal inflammation and marked microbiota encroachment.
31336403	5	38	theme	systemic	773:780	arg1	inflammation					782:793	systemic inflammation	773:793	systemic inflammation	773:793	HFD induced significant local colonic effects, despite no observed weight gain or systemic inflammation, as HFD causes epithelial upregulation of inducible nitric oxide synthase, which is counteracted by bilberry.
31336403	5	39	theme	colonic	721:727	arg1	effects					729:735	significant local colonic effects	703:735	significant local colonic effects	703:735	HFD induced significant local colonic effects, despite no observed weight gain or systemic inflammation, as HFD causes epithelial upregulation of inducible nitric oxide synthase, which is counteracted by bilberry.
31336403	9	40	from	populations	1379:1389	arg1	colon					1398:1402	the colon	1394:1402	the colon	1394:1402	CONCLUSION HFD expand firmly adherent mucus thickness and reduce mucus-foraging bacteria populations in the colon prior to obesity.
31336403	6	41	theme	Muc2	976:979	arg1	levels					966:971	the mRNA levels	957:971	the mRNA levels of Muc2 and Tff3	957:988	The firmly adherent mucus layer becomes thicker and the mRNA levels of Muc2 and Tff3 are increased by HFD with or without bilberry.
31336403	4	42	theme	low-fat	623:629	arg1	diet					631:634	a low-fat diet	621:634	a low-fat diet	621:634	METHODS AND RESULTS The effects on colonic mucus thickness in vivo and gut microbiota composition (Illumina sequencing, quantitative real-time PCR) are investigated in young rats fed a low-fat diet or HFD with or without bilberries for 8 weeks (n = 8).
31336403	7	43	theme	colonic	1066:1072	arg1	abundance					1074:1082	the colonic abundance	1062:1082	the colonic abundance of mucolytic bacterial species	1062:1113	In parallel, HFD reduced the colonic abundance of mucolytic bacterial species Akkermansia muciniphila and Bacteroides spp.
31336403	4	44	theme	gut	509:511	arg1	composition					524:534	gut microbiota composition	509:534	gut microbiota composition	509:534	METHODS AND RESULTS The effects on colonic mucus thickness in vivo and gut microbiota composition (Illumina sequencing, quantitative real-time PCR) are investigated in young rats fed a low-fat diet or HFD with or without bilberries for 8 weeks (n = 8).
31336403	4	45	theme	quantitative	558:569	arg1	PCR					581:583	quantitative real-time PCR	558:583	quantitative real-time PCR	558:583	METHODS AND RESULTS The effects on colonic mucus thickness in vivo and gut microbiota composition (Illumina sequencing, quantitative real-time PCR) are investigated in young rats fed a low-fat diet or HFD with or without bilberries for 8 weeks (n = 8).
31336403	4	45	theme	quantitative	558:569	arg1	sequencing					546:555	Illumina sequencing	537:555	Illumina sequencing	537:555	METHODS AND RESULTS The effects on colonic mucus thickness in vivo and gut microbiota composition (Illumina sequencing, quantitative real-time PCR) are investigated in young rats fed a low-fat diet or HFD with or without bilberries for 8 weeks (n = 8).
31336403	4	46	theme	young	606:610	arg1	rats					612:615	young rats	606:615	young rats fed a low-fat diet or HFD with or without bilberries for 8 weeks (n = 8)	606:688	METHODS AND RESULTS The effects on colonic mucus thickness in vivo and gut microbiota composition (Illumina sequencing, quantitative real-time PCR) are investigated in young rats fed a low-fat diet or HFD with or without bilberries for 8 weeks (n = 8).
31336403	5	47	theme	weight	758:763	arg1	gain					765:768	no observed weight gain	746:768	no observed weight gain	746:768	HFD induced significant local colonic effects, despite no observed weight gain or systemic inflammation, as HFD causes epithelial upregulation of inducible nitric oxide synthase, which is counteracted by bilberry.
31336403	0	48	theme	Mucus	54:58	arg1	Dynamics					60:67	Colonic Mucus Dynamics	46:67	Colonic Mucus Dynamics	46:67	High-Fat Diet Enriched with Bilberry Modifies Colonic Mucus Dynamics and Restores Marked Alterations of Gut Microbiome in Rats.
31336403	9	49	theme	adherent	1319:1326	arg1	thickness					1334:1342	firmly adherent mucus thickness	1312:1342	firmly adherent mucus thickness	1312:1342	CONCLUSION HFD expand firmly adherent mucus thickness and reduce mucus-foraging bacteria populations in the colon prior to obesity.
31336403	4	50	dep	quantitative	558:569	arg1	real-time					571:579	real-time	571:579	real-time	571:579	METHODS AND RESULTS The effects on colonic mucus thickness in vivo and gut microbiota composition (Illumina sequencing, quantitative real-time PCR) are investigated in young rats fed a low-fat diet or HFD with or without bilberries for 8 weeks (n = 8).
31336403	5	51	theme	oxide	854:858	arg1	synthase					860:867	inducible nitric oxide synthase	837:867	inducible nitric oxide synthase	837:867	HFD induced significant local colonic effects, despite no observed weight gain or systemic inflammation, as HFD causes epithelial upregulation of inducible nitric oxide synthase, which is counteracted by bilberry.
31336403	0	52	theme	Colonic	46:52	arg1	Dynamics					60:67	Colonic Mucus Dynamics	46:67	Colonic Mucus Dynamics	46:67	High-Fat Diet Enriched with Bilberry Modifies Colonic Mucus Dynamics and Restores Marked Alterations of Gut Microbiome in Rats.
31336403	4	53	dep	thickness	487:495	arg1	PCR					581:583	quantitative real-time PCR	558:583	quantitative real-time PCR	558:583	METHODS AND RESULTS The effects on colonic mucus thickness in vivo and gut microbiota composition (Illumina sequencing, quantitative real-time PCR) are investigated in young rats fed a low-fat diet or HFD with or without bilberries for 8 weeks (n = 8).
31336403	4	53	dep	thickness	487:495	arg1	sequencing					546:555	Illumina sequencing	537:555	Illumina sequencing	537:555	METHODS AND RESULTS The effects on colonic mucus thickness in vivo and gut microbiota composition (Illumina sequencing, quantitative real-time PCR) are investigated in young rats fed a low-fat diet or HFD with or without bilberries for 8 weeks (n = 8).
31336403	10	54	theme	microbiota	1502:1511	arg1	encroachment					1513:1524	marked microbiota encroachment	1495:1524	marked microbiota encroachment	1495:1524	Enriching HFD with bilberry protects against intestinal inflammation and marked microbiota encroachment.
31336403	0	55	from	Microbiome	108:117	arg1	Rats					122:125	Rats	122:125	Rats	122:125	High-Fat Diet Enriched with Bilberry Modifies Colonic Mucus Dynamics and Restores Marked Alterations of Gut Microbiome in Rats.
31336403	5	56	theme	synthase	860:867	arg1	upregulation					821:832	epithelial upregulation	810:832	epithelial upregulation of inducible nitric oxide synthase, which is counteracted by bilberry	810:902	HFD induced significant local colonic effects, despite no observed weight gain or systemic inflammation, as HFD causes epithelial upregulation of inducible nitric oxide synthase, which is counteracted by bilberry.
31336403	4	57	theme	microbiota	513:522	arg1	composition					524:534	gut microbiota composition	509:534	gut microbiota composition	509:534	METHODS AND RESULTS The effects on colonic mucus thickness in vivo and gut microbiota composition (Illumina sequencing, quantitative real-time PCR) are investigated in young rats fed a low-fat diet or HFD with or without bilberries for 8 weeks (n = 8).
31336403	10	58	theme	marked	1495:1500	arg1	encroachment					1513:1524	marked microbiota encroachment	1495:1524	marked microbiota encroachment	1495:1524	Enriching HFD with bilberry protects against intestinal inflammation and marked microbiota encroachment.
31336403	6	59	theme	mRNA	961:964	arg1	levels					966:971	the mRNA levels	957:971	the mRNA levels of Muc2 and Tff3	957:988	The firmly adherent mucus layer becomes thicker and the mRNA levels of Muc2 and Tff3 are increased by HFD with or without bilberry.
31336403	1	60	theme	gut	205:207	arg1	dysbiosis					220:228	gut microbiome dysbiosis	205:228	gut microbiome dysbiosis	205:228	SCOPE Emerging evidence suggests that high-fat diet (HFD) is associated with gut microbiome dysbiosis and related disorders.
31336403	1	61	theme	SCOPE	128:132	arg1	evidence					143:150	SCOPE Emerging evidence	128:150	SCOPE Emerging evidence	128:150	SCOPE Emerging evidence suggests that high-fat diet (HFD) is associated with gut microbiome dysbiosis and related disorders.
31336403	1	62	theme	microbiome	209:218	arg1	dysbiosis					220:228	gut microbiome dysbiosis	205:228	gut microbiome dysbiosis	205:228	SCOPE Emerging evidence suggests that high-fat diet (HFD) is associated with gut microbiome dysbiosis and related disorders.
31336403	0	63	from	Alterations	89:99	arg1	Rats					122:125	Rats	122:125	Rats	122:125	High-Fat Diet Enriched with Bilberry Modifies Colonic Mucus Dynamics and Restores Marked Alterations of Gut Microbiome in Rats.
31336403	1	64	theme	Emerging	134:141	arg1	evidence					143:150	SCOPE Emerging evidence	128:150	SCOPE Emerging evidence	128:150	SCOPE Emerging evidence suggests that high-fat diet (HFD) is associated with gut microbiome dysbiosis and related disorders.
31336403	0	65	from	Rats	122:125	arg1	Alterations					89:99	Marked Alterations	82:99	Marked Alterations of Gut Microbiome in Rats	82:125	High-Fat Diet Enriched with Bilberry Modifies Colonic Mucus Dynamics and Restores Marked Alterations of Gut Microbiome in Rats.
31336403	3	66	theme	microbiome	372:381	arg1	dynamics					332:339	the dynamics	328:339	the dynamics of the colonic mucus layer and microbiome during HFD and bilberry supplementation	328:421	Herein, the dynamics of the colonic mucus layer and microbiome during HFD and bilberry supplementation are addressed.
31336403	7	67	theme	mucolytic	1087:1095	arg1	species					1107:1113	mucolytic bacterial species	1087:1113	mucolytic bacterial species	1087:1113	In parallel, HFD reduced the colonic abundance of mucolytic bacterial species Akkermansia muciniphila and Bacteroides spp.
31336403	7	68	theme	bacterial	1097:1105	arg1	species					1107:1113	mucolytic bacterial species	1087:1113	mucolytic bacterial species	1087:1113	In parallel, HFD reduced the colonic abundance of mucolytic bacterial species Akkermansia muciniphila and Bacteroides spp.
31336403	9	69	theme	bacteria	1370:1377	arg1	populations					1379:1389	mucus-foraging bacteria populations	1355:1389	mucus-foraging bacteria populations in the colon prior to obesity	1355:1419	CONCLUSION HFD expand firmly adherent mucus thickness and reduce mucus-foraging bacteria populations in the colon prior to obesity.
29544868	9	0	theme	diabetic	1706:1713	arg1	HAECs					1715:1719	diabetic HAECs	1706:1719	diabetic HAECs	1706:1719	However, treatment with BBM restored the levels of GAGs in diabetic HAECs.
29544868	3	1	theme	hydroxyhippuric	683:697	arg1	acid					699:702	hydroxyhippuric acid	683:702	hydroxyhippuric acid	683:702	METHODS ECs isolated from healthy individuals [human aortic ECs (HAECs)] and diabetic patients (diabetic HAECs) were treated with ±BBM (benzoic acid-4-sulfate, hippuric acid, hydroxyhippuric acid, isovanillic acid-3-sulfate, and vanillic acid-4-sulfate at concentrations known to circulate in human plasma following blueberry consumption) for 3 days, and indices for endothelial inflammation were measured.
29544868	6	2	theme	PA1	1345:1347	arg1	column					1349:1354	Dionex CarboPac PA1 column	1329:1354	Dionex CarboPac PA1 column	1329:1354	Heparan sulfate/chondroitin sulfate ratio and disaccharide composition of GAGs from the medium were analyzed using DEAE-3SW column and Dionex CarboPac PA1 column, respectively.
29544868	2	3	theme	blueberry	376:384	arg1	metabolites					386:396	blueberry metabolites	376:396	blueberry metabolites (BBM)	376:402	We tested the hypothesis that blueberry metabolites (BBM) ameliorate endothelial inflammation in diabetic endothelial cells (ECs) by restoring cell surface GAGs.
29544868	2	3	theme	blueberry	376:384	arg1	BBM					399:401	BBM	399:401	BBM	399:401	We tested the hypothesis that blueberry metabolites (BBM) ameliorate endothelial inflammation in diabetic endothelial cells (ECs) by restoring cell surface GAGs.
29544868	6	4	theme	disaccharide	1240:1251	arg1	composition					1253:1263	disaccharide composition	1240:1263	disaccharide composition	1240:1263	Heparan sulfate/chondroitin sulfate ratio and disaccharide composition of GAGs from the medium were analyzed using DEAE-3SW column and Dionex CarboPac PA1 column, respectively.
29544868	6	5	theme	Dionex	1329:1334	arg1	column					1349:1354	Dionex CarboPac PA1 column	1329:1354	Dionex CarboPac PA1 column	1329:1354	Heparan sulfate/chondroitin sulfate ratio and disaccharide composition of GAGs from the medium were analyzed using DEAE-3SW column and Dionex CarboPac PA1 column, respectively.
29544868	1	6	theme	endothelial	287:297	arg1	inflammation					299:310	endothelial inflammation	287:310	endothelial inflammation	287:310	BACKGROUND Glycosaminoglycan (GAG), a major component of the endothelial glycocalyx, is severely perturbed in diabetic vasculature leading to endothelial inflammation and vascular disease in diabetes.
29544868	7	7	theme	diabetic	1490:1497	arg1	HAECs					1499:1503	diabetic HAECs	1490:1503	diabetic HAECs	1490:1503	RESULTS BBM suppressed diabetes-induced monocyte binding to ECs, and reduced the expression of inflammatory markers in diabetic HAECs.
29544868	1	8	from	disease	325:331	arg1	diabetes					336:343	diabetes	336:343	diabetes	336:343	BACKGROUND Glycosaminoglycan (GAG), a major component of the endothelial glycocalyx, is severely perturbed in diabetic vasculature leading to endothelial inflammation and vascular disease in diabetes.
29544868	6	9	theme	sulfate/chondroitin	1202:1220	arg1	sulfate					1222:1228	Heparan sulfate/chondroitin sulfate	1194:1228	Heparan sulfate/chondroitin sulfate ratio	1194:1234	Heparan sulfate/chondroitin sulfate ratio and disaccharide composition of GAGs from the medium were analyzed using DEAE-3SW column and Dionex CarboPac PA1 column, respectively.
29544868	9	10	with	treatment	1656:1664	arg1	BBM					1671:1673	BBM	1671:1673	BBM	1671:1673	However, treatment with BBM restored the levels of GAGs in diabetic HAECs.
29544868	11	11	theme	cell	1883:1886	arg1	GAGs					1896:1899	cell surface GAGs	1883:1899	cell surface GAGs	1883:1899	CONCLUSIONS BBM restored cell surface GAGs and attenuated endothelial inflammation in diabetic HAECs.
29544868	3	12	theme	diabetic	585:592	arg1	patients					594:601	diabetic patients	585:601	diabetic patients	585:601	METHODS ECs isolated from healthy individuals [human aortic ECs (HAECs)] and diabetic patients (diabetic HAECs) were treated with ±BBM (benzoic acid-4-sulfate, hippuric acid, hydroxyhippuric acid, isovanillic acid-3-sulfate, and vanillic acid-4-sulfate at concentrations known to circulate in human plasma following blueberry consumption) for 3 days, and indices for endothelial inflammation were measured.
29544868	10	13	theme	disaccharide	1786:1797	arg1	composition					1799:1809	disaccharide composition	1786:1809	disaccharide composition	1786:1809	The composition, heparan sulfate/chondroitin sulfate ratio, and disaccharide composition of GAGs from medium were similar among groups.
29544868	1	14	theme	glycocalyx	218:227	arg1	Glycosaminoglycan					156:172	BACKGROUND Glycosaminoglycan	145:172	BACKGROUND Glycosaminoglycan (GAG)	145:178	BACKGROUND Glycosaminoglycan (GAG), a major component of the endothelial glycocalyx, is severely perturbed in diabetic vasculature leading to endothelial inflammation and vascular disease in diabetes.
29544868	1	14	theme	glycocalyx	218:227	arg1	component					189:197	a major component	181:197	a major component of the endothelial glycocalyx	181:227	BACKGROUND Glycosaminoglycan (GAG), a major component of the endothelial glycocalyx, is severely perturbed in diabetic vasculature leading to endothelial inflammation and vascular disease in diabetes.
29544868	1	15	theme	BACKGROUND	145:154	arg1	Glycosaminoglycan					156:172	BACKGROUND Glycosaminoglycan	145:172	BACKGROUND Glycosaminoglycan (GAG)	145:178	BACKGROUND Glycosaminoglycan (GAG), a major component of the endothelial glycocalyx, is severely perturbed in diabetic vasculature leading to endothelial inflammation and vascular disease in diabetes.
29544868	1	15	theme	BACKGROUND	145:154	arg1	component					189:197	a major component	181:197	a major component of the endothelial glycocalyx	181:227	BACKGROUND Glycosaminoglycan (GAG), a major component of the endothelial glycocalyx, is severely perturbed in diabetic vasculature leading to endothelial inflammation and vascular disease in diabetes.
29544868	1	15	theme	BACKGROUND	145:154	arg1	GAG					175:177	GAG	175:177	GAG	175:177	BACKGROUND Glycosaminoglycan (GAG), a major component of the endothelial glycocalyx, is severely perturbed in diabetic vasculature leading to endothelial inflammation and vascular disease in diabetes.
29544868	10	16	from	medium	1824:1829	arg1	composition					1799:1809	disaccharide composition	1786:1809	disaccharide composition	1786:1809	The composition, heparan sulfate/chondroitin sulfate ratio, and disaccharide composition of GAGs from medium were similar among groups.
29544868	10	16	from	medium	1824:1829	arg1	similar					1836:1842	similar	1836:1842	similar	1836:1842	The composition, heparan sulfate/chondroitin sulfate ratio, and disaccharide composition of GAGs from medium were similar among groups.
29544868	10	16	from	medium	1824:1829	arg1	ratio					1775:1779	heparan sulfate/chondroitin sulfate ratio	1739:1779	heparan sulfate/chondroitin sulfate ratio	1739:1779	The composition, heparan sulfate/chondroitin sulfate ratio, and disaccharide composition of GAGs from medium were similar among groups.
29544868	10	16	from	medium	1824:1829	arg1	composition					1726:1736	The composition	1722:1736	The composition	1722:1736	The composition, heparan sulfate/chondroitin sulfate ratio, and disaccharide composition of GAGs from medium were similar among groups.
29544868	3	17	theme	blueberry	824:832	arg1	consumption					834:844	blueberry consumption	824:844	blueberry consumption	824:844	METHODS ECs isolated from healthy individuals [human aortic ECs (HAECs)] and diabetic patients (diabetic HAECs) were treated with ±BBM (benzoic acid-4-sulfate, hippuric acid, hydroxyhippuric acid, isovanillic acid-3-sulfate, and vanillic acid-4-sulfate at concentrations known to circulate in human plasma following blueberry consumption) for 3 days, and indices for endothelial inflammation were measured.
29544868	0	18	theme	endothelial	76:86	arg1	inflammation					88:99	endothelial inflammation	76:99	endothelial inflammation in diabetic human aortic endothelial cells	76:142	Blueberry metabolites restore cell surface glycosaminoglycans and attenuate endothelial inflammation in diabetic human aortic endothelial cells.
29544868	5	19	theme	flow	1165:1168	arg1	analyzer					1184:1191	an inline flow scintillation analyzer	1155:1191	an inline flow scintillation analyzer	1155:1191	Total GAGs in ECs and medium were purified using DEAE-Sepharose column and were analyzed with high-pressure liquid chromatography coupled to an inline flow scintillation analyzer.
29544868	12	20	theme	vascular	2021:2028	arg1	complications					2030:2042	vascular complications	2021:2042	vascular complications in diabetes	2021:2054	Blueberry might complement conventional therapies to improve vascular complications in diabetes.
29544868	0	21	theme	human	113:117	arg1	cells					138:142	diabetic human aortic endothelial cells	104:142	diabetic human aortic endothelial cells	104:142	Blueberry metabolites restore cell surface glycosaminoglycans and attenuate endothelial inflammation in diabetic human aortic endothelial cells.
29544868	5	22	theme	liquid	1122:1127	arg1	chromatography					1129:1142	high-pressure liquid chromatography	1108:1142	high-pressure liquid chromatography coupled to an inline flow scintillation analyzer	1108:1191	Total GAGs in ECs and medium were purified using DEAE-Sepharose column and were analyzed with high-pressure liquid chromatography coupled to an inline flow scintillation analyzer.
29544868	3	23	dep	METHODS	508:514	arg1	ECs					516:518	ECs	516:518	METHODS ECs isolated from healthy individuals [human aortic ECs (HAECs)] and diabetic patients (diabetic HAECs)	508:618	METHODS ECs isolated from healthy individuals [human aortic ECs (HAECs)] and diabetic patients (diabetic HAECs) were treated with ±BBM (benzoic acid-4-sulfate, hippuric acid, hydroxyhippuric acid, isovanillic acid-3-sulfate, and vanillic acid-4-sulfate at concentrations known to circulate in human plasma following blueberry consumption) for 3 days, and indices for endothelial inflammation were measured.
29544868	3	23	dep	METHODS	508:514	arg1	patients					594:601	diabetic patients	585:601	diabetic patients	585:601	METHODS ECs isolated from healthy individuals [human aortic ECs (HAECs)] and diabetic patients (diabetic HAECs) were treated with ±BBM (benzoic acid-4-sulfate, hippuric acid, hydroxyhippuric acid, isovanillic acid-3-sulfate, and vanillic acid-4-sulfate at concentrations known to circulate in human plasma following blueberry consumption) for 3 days, and indices for endothelial inflammation were measured.
29544868	3	23	dep	METHODS	508:514	arg1	HAECs					573:577	HAECs	573:577	HAECs	573:577	METHODS ECs isolated from healthy individuals [human aortic ECs (HAECs)] and diabetic patients (diabetic HAECs) were treated with ±BBM (benzoic acid-4-sulfate, hippuric acid, hydroxyhippuric acid, isovanillic acid-3-sulfate, and vanillic acid-4-sulfate at concentrations known to circulate in human plasma following blueberry consumption) for 3 days, and indices for endothelial inflammation were measured.
29544868	3	23	dep	METHODS	508:514	arg1	ECs					568:570	[human aortic ECs	554:570	[human aortic ECs (HAECs)]	554:579	METHODS ECs isolated from healthy individuals [human aortic ECs (HAECs)] and diabetic patients (diabetic HAECs) were treated with ±BBM (benzoic acid-4-sulfate, hippuric acid, hydroxyhippuric acid, isovanillic acid-3-sulfate, and vanillic acid-4-sulfate at concentrations known to circulate in human plasma following blueberry consumption) for 3 days, and indices for endothelial inflammation were measured.
29544868	8	24	theme	surface	1587:1593	arg1	GAGs					1595:1598	the cell surface GAGs	1578:1598	the cell surface GAGs indicating the dysregulation of sulfated GAGs	1578:1644	Diabetic HAECs displayed a decrease in [35S] sulfate incorporation into the cell surface GAGs indicating the dysregulation of sulfated GAGs.
29544868	2	25	theme	endothelial	452:462	arg1	ECs					471:473	ECs	471:473	ECs	471:473	We tested the hypothesis that blueberry metabolites (BBM) ameliorate endothelial inflammation in diabetic endothelial cells (ECs) by restoring cell surface GAGs.
29544868	2	25	theme	endothelial	452:462	arg1	cells					464:468	diabetic endothelial cells	443:468	diabetic endothelial cells (ECs)	443:474	We tested the hypothesis that blueberry metabolites (BBM) ameliorate endothelial inflammation in diabetic endothelial cells (ECs) by restoring cell surface GAGs.
29544868	0	26	theme	endothelial	126:136	arg1	cells					138:142	diabetic human aortic endothelial cells	104:142	diabetic human aortic endothelial cells	104:142	Blueberry metabolites restore cell surface glycosaminoglycans and attenuate endothelial inflammation in diabetic human aortic endothelial cells.
29544868	12	27	theme	conventional	1987:1998	arg1	therapies					2000:2008	conventional therapies	1987:2008	conventional therapies	1987:2008	Blueberry might complement conventional therapies to improve vascular complications in diabetes.
29544868	9	28	from	levels	1688:1693	arg1	HAECs					1715:1719	diabetic HAECs	1706:1719	diabetic HAECs	1706:1719	However, treatment with BBM restored the levels of GAGs in diabetic HAECs.
29544868	7	29	theme	monocyte	1411:1418	arg1	binding					1420:1426	diabetes-induced monocyte binding	1394:1426	diabetes-induced monocyte binding to ECs	1394:1433	RESULTS BBM suppressed diabetes-induced monocyte binding to ECs, and reduced the expression of inflammatory markers in diabetic HAECs.
29544868	3	30	from	concentrations	764:777	arg1	acid-4-sulfate					746:759	vanillic acid-4-sulfate	737:759	vanillic acid-4-sulfate	737:759	METHODS ECs isolated from healthy individuals [human aortic ECs (HAECs)] and diabetic patients (diabetic HAECs) were treated with ±BBM (benzoic acid-4-sulfate, hippuric acid, hydroxyhippuric acid, isovanillic acid-3-sulfate, and vanillic acid-4-sulfate at concentrations known to circulate in human plasma following blueberry consumption) for 3 days, and indices for endothelial inflammation were measured.
29544868	3	30	from	concentrations	764:777	arg1	acid-4-sulfate					652:665	benzoic acid-4-sulfate	644:665	benzoic acid-4-sulfate	644:665	METHODS ECs isolated from healthy individuals [human aortic ECs (HAECs)] and diabetic patients (diabetic HAECs) were treated with ±BBM (benzoic acid-4-sulfate, hippuric acid, hydroxyhippuric acid, isovanillic acid-3-sulfate, and vanillic acid-4-sulfate at concentrations known to circulate in human plasma following blueberry consumption) for 3 days, and indices for endothelial inflammation were measured.
29544868	3	30	from	concentrations	764:777	arg1	acid					677:680	hippuric acid	668:680	hippuric acid	668:680	METHODS ECs isolated from healthy individuals [human aortic ECs (HAECs)] and diabetic patients (diabetic HAECs) were treated with ±BBM (benzoic acid-4-sulfate, hippuric acid, hydroxyhippuric acid, isovanillic acid-3-sulfate, and vanillic acid-4-sulfate at concentrations known to circulate in human plasma following blueberry consumption) for 3 days, and indices for endothelial inflammation were measured.
29544868	3	30	from	concentrations	764:777	arg1	acid					699:702	hydroxyhippuric acid	683:702	hydroxyhippuric acid	683:702	METHODS ECs isolated from healthy individuals [human aortic ECs (HAECs)] and diabetic patients (diabetic HAECs) were treated with ±BBM (benzoic acid-4-sulfate, hippuric acid, hydroxyhippuric acid, isovanillic acid-3-sulfate, and vanillic acid-4-sulfate at concentrations known to circulate in human plasma following blueberry consumption) for 3 days, and indices for endothelial inflammation were measured.
29544868	3	30	from	concentrations	764:777	arg1	acid-3-sulfate					717:730	isovanillic acid-3-sulfate	705:730	isovanillic acid-3-sulfate	705:730	METHODS ECs isolated from healthy individuals [human aortic ECs (HAECs)] and diabetic patients (diabetic HAECs) were treated with ±BBM (benzoic acid-4-sulfate, hippuric acid, hydroxyhippuric acid, isovanillic acid-3-sulfate, and vanillic acid-4-sulfate at concentrations known to circulate in human plasma following blueberry consumption) for 3 days, and indices for endothelial inflammation were measured.
29544868	0	31	theme	Blueberry	0:8	arg1	metabolites					10:20	Blueberry metabolites	0:20	Blueberry metabolites	0:20	Blueberry metabolites restore cell surface glycosaminoglycans and attenuate endothelial inflammation in diabetic human aortic endothelial cells.
29544868	10	32	theme	sulfate/chondroitin	1747:1765	arg1	sulfate					1767:1773	heparan sulfate/chondroitin sulfate	1739:1773	heparan sulfate/chondroitin sulfate ratio	1739:1779	The composition, heparan sulfate/chondroitin sulfate ratio, and disaccharide composition of GAGs from medium were similar among groups.
29544868	1	33	theme	vascular	316:323	arg1	disease					325:331	vascular disease	316:331	vascular disease	316:331	BACKGROUND Glycosaminoglycan (GAG), a major component of the endothelial glycocalyx, is severely perturbed in diabetic vasculature leading to endothelial inflammation and vascular disease in diabetes.
29544868	7	34	theme	RESULTS	1371:1377	arg1	BBM					1379:1381	RESULTS BBM	1371:1381	RESULTS BBM	1371:1381	RESULTS BBM suppressed diabetes-induced monocyte binding to ECs, and reduced the expression of inflammatory markers in diabetic HAECs.
29544868	8	35	theme	sulfated	1632:1639	arg1	GAGs					1641:1644	sulfated GAGs	1632:1644	sulfated GAGs	1632:1644	Diabetic HAECs displayed a decrease in [35S] sulfate incorporation into the cell surface GAGs indicating the dysregulation of sulfated GAGs.
29544868	2	36	theme	diabetic	443:450	arg1	ECs					471:473	ECs	471:473	ECs	471:473	We tested the hypothesis that blueberry metabolites (BBM) ameliorate endothelial inflammation in diabetic endothelial cells (ECs) by restoring cell surface GAGs.
29544868	2	36	theme	diabetic	443:450	arg1	cells					464:468	diabetic endothelial cells	443:468	diabetic endothelial cells (ECs)	443:474	We tested the hypothesis that blueberry metabolites (BBM) ameliorate endothelial inflammation in diabetic endothelial cells (ECs) by restoring cell surface GAGs.
29544868	0	37	theme	surface	35:41	arg1	glycosaminoglycans					43:60	cell surface glycosaminoglycans	30:60	cell surface glycosaminoglycans	30:60	Blueberry metabolites restore cell surface glycosaminoglycans and attenuate endothelial inflammation in diabetic human aortic endothelial cells.
29544868	2	38	theme	cell	489:492	arg1	GAGs					502:505	cell surface GAGs	489:505	cell surface GAGs	489:505	We tested the hypothesis that blueberry metabolites (BBM) ameliorate endothelial inflammation in diabetic endothelial cells (ECs) by restoring cell surface GAGs.
29544868	11	39	theme	diabetic	1944:1951	arg1	HAECs					1953:1957	diabetic HAECs	1944:1957	diabetic HAECs	1944:1957	CONCLUSIONS BBM restored cell surface GAGs and attenuated endothelial inflammation in diabetic HAECs.
29544868	2	40	from	inflammation	427:438	arg1	ECs					471:473	ECs	471:473	ECs	471:473	We tested the hypothesis that blueberry metabolites (BBM) ameliorate endothelial inflammation in diabetic endothelial cells (ECs) by restoring cell surface GAGs.
29544868	2	40	from	inflammation	427:438	arg1	cells					464:468	diabetic endothelial cells	443:468	diabetic endothelial cells (ECs)	443:474	We tested the hypothesis that blueberry metabolites (BBM) ameliorate endothelial inflammation in diabetic endothelial cells (ECs) by restoring cell surface GAGs.
29544868	3	41	dep	±BBM	638:641	arg1	acid-4-sulfate					746:759	vanillic acid-4-sulfate	737:759	vanillic acid-4-sulfate	737:759	METHODS ECs isolated from healthy individuals [human aortic ECs (HAECs)] and diabetic patients (diabetic HAECs) were treated with ±BBM (benzoic acid-4-sulfate, hippuric acid, hydroxyhippuric acid, isovanillic acid-3-sulfate, and vanillic acid-4-sulfate at concentrations known to circulate in human plasma following blueberry consumption) for 3 days, and indices for endothelial inflammation were measured.
29544868	3	41	dep	±BBM	638:641	arg1	acid-4-sulfate					652:665	benzoic acid-4-sulfate	644:665	benzoic acid-4-sulfate	644:665	METHODS ECs isolated from healthy individuals [human aortic ECs (HAECs)] and diabetic patients (diabetic HAECs) were treated with ±BBM (benzoic acid-4-sulfate, hippuric acid, hydroxyhippuric acid, isovanillic acid-3-sulfate, and vanillic acid-4-sulfate at concentrations known to circulate in human plasma following blueberry consumption) for 3 days, and indices for endothelial inflammation were measured.
29544868	3	41	dep	±BBM	638:641	arg1	acid					677:680	hippuric acid	668:680	hippuric acid	668:680	METHODS ECs isolated from healthy individuals [human aortic ECs (HAECs)] and diabetic patients (diabetic HAECs) were treated with ±BBM (benzoic acid-4-sulfate, hippuric acid, hydroxyhippuric acid, isovanillic acid-3-sulfate, and vanillic acid-4-sulfate at concentrations known to circulate in human plasma following blueberry consumption) for 3 days, and indices for endothelial inflammation were measured.
29544868	3	41	dep	±BBM	638:641	arg1	acid					699:702	hydroxyhippuric acid	683:702	hydroxyhippuric acid	683:702	METHODS ECs isolated from healthy individuals [human aortic ECs (HAECs)] and diabetic patients (diabetic HAECs) were treated with ±BBM (benzoic acid-4-sulfate, hippuric acid, hydroxyhippuric acid, isovanillic acid-3-sulfate, and vanillic acid-4-sulfate at concentrations known to circulate in human plasma following blueberry consumption) for 3 days, and indices for endothelial inflammation were measured.
29544868	3	41	dep	±BBM	638:641	arg1	acid-3-sulfate					717:730	isovanillic acid-3-sulfate	705:730	isovanillic acid-3-sulfate	705:730	METHODS ECs isolated from healthy individuals [human aortic ECs (HAECs)] and diabetic patients (diabetic HAECs) were treated with ±BBM (benzoic acid-4-sulfate, hippuric acid, hydroxyhippuric acid, isovanillic acid-3-sulfate, and vanillic acid-4-sulfate at concentrations known to circulate in human plasma following blueberry consumption) for 3 days, and indices for endothelial inflammation were measured.
29544868	3	42	attach	isolated	520:527	arg1	individuals					542:552	healthy individuals	534:552	healthy individuals	534:552	METHODS ECs isolated from healthy individuals [human aortic ECs (HAECs)] and diabetic patients (diabetic HAECs) were treated with ±BBM (benzoic acid-4-sulfate, hippuric acid, hydroxyhippuric acid, isovanillic acid-3-sulfate, and vanillic acid-4-sulfate at concentrations known to circulate in human plasma following blueberry consumption) for 3 days, and indices for endothelial inflammation were measured.
29544868	3	42	attach	isolated	520:527	arg2	ECs					516:518	ECs	516:518	METHODS ECs isolated from healthy individuals [human aortic ECs (HAECs)] and diabetic patients (diabetic HAECs)	508:618	METHODS ECs isolated from healthy individuals [human aortic ECs (HAECs)] and diabetic patients (diabetic HAECs) were treated with ±BBM (benzoic acid-4-sulfate, hippuric acid, hydroxyhippuric acid, isovanillic acid-3-sulfate, and vanillic acid-4-sulfate at concentrations known to circulate in human plasma following blueberry consumption) for 3 days, and indices for endothelial inflammation were measured.
29544868	3	43	theme	hippuric	668:675	arg1	acid					677:680	hippuric acid	668:680	hippuric acid	668:680	METHODS ECs isolated from healthy individuals [human aortic ECs (HAECs)] and diabetic patients (diabetic HAECs) were treated with ±BBM (benzoic acid-4-sulfate, hippuric acid, hydroxyhippuric acid, isovanillic acid-3-sulfate, and vanillic acid-4-sulfate at concentrations known to circulate in human plasma following blueberry consumption) for 3 days, and indices for endothelial inflammation were measured.
29544868	8	44	theme	Diabetic	1506:1513	arg1	HAECs					1515:1519	Diabetic HAECs	1506:1519	Diabetic HAECs	1506:1519	Diabetic HAECs displayed a decrease in [35S] sulfate incorporation into the cell surface GAGs indicating the dysregulation of sulfated GAGs.
29544868	10	45	theme	GAGs	1814:1817	arg1	composition					1799:1809	disaccharide composition	1786:1809	disaccharide composition	1786:1809	The composition, heparan sulfate/chondroitin sulfate ratio, and disaccharide composition of GAGs from medium were similar among groups.
29544868	10	45	theme	GAGs	1814:1817	arg1	similar					1836:1842	similar	1836:1842	similar	1836:1842	The composition, heparan sulfate/chondroitin sulfate ratio, and disaccharide composition of GAGs from medium were similar among groups.
29544868	10	45	theme	GAGs	1814:1817	arg1	ratio					1775:1779	heparan sulfate/chondroitin sulfate ratio	1739:1779	heparan sulfate/chondroitin sulfate ratio	1739:1779	The composition, heparan sulfate/chondroitin sulfate ratio, and disaccharide composition of GAGs from medium were similar among groups.
29544868	10	45	theme	GAGs	1814:1817	arg1	composition					1726:1736	The composition	1722:1736	The composition	1722:1736	The composition, heparan sulfate/chondroitin sulfate ratio, and disaccharide composition of GAGs from medium were similar among groups.
29544868	3	46	theme	isovanillic	705:715	arg1	acid-3-sulfate					717:730	isovanillic acid-3-sulfate	705:730	isovanillic acid-3-sulfate	705:730	METHODS ECs isolated from healthy individuals [human aortic ECs (HAECs)] and diabetic patients (diabetic HAECs) were treated with ±BBM (benzoic acid-4-sulfate, hippuric acid, hydroxyhippuric acid, isovanillic acid-3-sulfate, and vanillic acid-4-sulfate at concentrations known to circulate in human plasma following blueberry consumption) for 3 days, and indices for endothelial inflammation were measured.
29544868	6	47	theme	DEAE-3SW	1309:1316	arg1	column					1318:1323	DEAE-3SW column	1309:1323	DEAE-3SW column	1309:1323	Heparan sulfate/chondroitin sulfate ratio and disaccharide composition of GAGs from the medium were analyzed using DEAE-3SW column and Dionex CarboPac PA1 column, respectively.
29544868	6	48	theme	CarboPac	1336:1343	arg1	column					1349:1354	Dionex CarboPac PA1 column	1329:1354	Dionex CarboPac PA1 column	1329:1354	Heparan sulfate/chondroitin sulfate ratio and disaccharide composition of GAGs from the medium were analyzed using DEAE-3SW column and Dionex CarboPac PA1 column, respectively.
29544868	7	49	theme	markers	1479:1485	arg1	expression					1452:1461	the expression	1448:1461	the expression of inflammatory markers in diabetic HAECs	1448:1503	RESULTS BBM suppressed diabetes-induced monocyte binding to ECs, and reduced the expression of inflammatory markers in diabetic HAECs.
29544868	3	50	theme	vanillic	737:744	arg1	acid-4-sulfate					746:759	vanillic acid-4-sulfate	737:759	vanillic acid-4-sulfate	737:759	METHODS ECs isolated from healthy individuals [human aortic ECs (HAECs)] and diabetic patients (diabetic HAECs) were treated with ±BBM (benzoic acid-4-sulfate, hippuric acid, hydroxyhippuric acid, isovanillic acid-3-sulfate, and vanillic acid-4-sulfate at concentrations known to circulate in human plasma following blueberry consumption) for 3 days, and indices for endothelial inflammation were measured.
29544868	3	51	theme	aortic	561:566	arg1	HAECs					573:577	HAECs	573:577	HAECs	573:577	METHODS ECs isolated from healthy individuals [human aortic ECs (HAECs)] and diabetic patients (diabetic HAECs) were treated with ±BBM (benzoic acid-4-sulfate, hippuric acid, hydroxyhippuric acid, isovanillic acid-3-sulfate, and vanillic acid-4-sulfate at concentrations known to circulate in human plasma following blueberry consumption) for 3 days, and indices for endothelial inflammation were measured.
29544868	3	51	theme	aortic	561:566	arg1	ECs					568:570	[human aortic ECs	554:570	[human aortic ECs (HAECs)]	554:579	METHODS ECs isolated from healthy individuals [human aortic ECs (HAECs)] and diabetic patients (diabetic HAECs) were treated with ±BBM (benzoic acid-4-sulfate, hippuric acid, hydroxyhippuric acid, isovanillic acid-3-sulfate, and vanillic acid-4-sulfate at concentrations known to circulate in human plasma following blueberry consumption) for 3 days, and indices for endothelial inflammation were measured.
29544868	1	52	theme	major	183:187	arg1	Glycosaminoglycan					156:172	BACKGROUND Glycosaminoglycan	145:172	BACKGROUND Glycosaminoglycan (GAG)	145:178	BACKGROUND Glycosaminoglycan (GAG), a major component of the endothelial glycocalyx, is severely perturbed in diabetic vasculature leading to endothelial inflammation and vascular disease in diabetes.
29544868	1	52	theme	major	183:187	arg1	component					189:197	a major component	181:197	a major component of the endothelial glycocalyx	181:227	BACKGROUND Glycosaminoglycan (GAG), a major component of the endothelial glycocalyx, is severely perturbed in diabetic vasculature leading to endothelial inflammation and vascular disease in diabetes.
29544868	6	53	theme	sulfate	1222:1228	arg1	ratio					1230:1234	Heparan sulfate/chondroitin sulfate ratio	1194:1234	Heparan sulfate/chondroitin sulfate ratio	1194:1234	Heparan sulfate/chondroitin sulfate ratio and disaccharide composition of GAGs from the medium were analyzed using DEAE-3SW column and Dionex CarboPac PA1 column, respectively.
29544868	1	54	from	inflammation	299:310	arg1	diabetes					336:343	diabetes	336:343	diabetes	336:343	BACKGROUND Glycosaminoglycan (GAG), a major component of the endothelial glycocalyx, is severely perturbed in diabetic vasculature leading to endothelial inflammation and vascular disease in diabetes.
29544868	5	55	from	GAGs	1020:1023	arg1	medium					1036:1041	medium	1036:1041	medium	1036:1041	Total GAGs in ECs and medium were purified using DEAE-Sepharose column and were analyzed with high-pressure liquid chromatography coupled to an inline flow scintillation analyzer.
29544868	5	55	from	GAGs	1020:1023	arg1	ECs					1028:1030	ECs	1028:1030	ECs	1028:1030	Total GAGs in ECs and medium were purified using DEAE-Sepharose column and were analyzed with high-pressure liquid chromatography coupled to an inline flow scintillation analyzer.
29544868	6	56	theme	Heparan	1194:1200	arg1	sulfate					1222:1228	Heparan sulfate/chondroitin sulfate	1194:1228	Heparan sulfate/chondroitin sulfate ratio	1194:1234	Heparan sulfate/chondroitin sulfate ratio and disaccharide composition of GAGs from the medium were analyzed using DEAE-3SW column and Dionex CarboPac PA1 column, respectively.
29544868	11	57	theme	surface	1888:1894	arg1	GAGs					1896:1899	cell surface GAGs	1883:1899	cell surface GAGs	1883:1899	CONCLUSIONS BBM restored cell surface GAGs and attenuated endothelial inflammation in diabetic HAECs.
29544868	1	58	theme	endothelial	206:216	arg1	glycocalyx					218:227	the endothelial glycocalyx	202:227	the endothelial glycocalyx	202:227	BACKGROUND Glycosaminoglycan (GAG), a major component of the endothelial glycocalyx, is severely perturbed in diabetic vasculature leading to endothelial inflammation and vascular disease in diabetes.
29544868	7	59	theme	inflammatory	1466:1477	arg1	markers					1479:1485	inflammatory markers	1466:1485	inflammatory markers	1466:1485	RESULTS BBM suppressed diabetes-induced monocyte binding to ECs, and reduced the expression of inflammatory markers in diabetic HAECs.
29544868	3	60	theme	diabetic	604:611	arg1	HAECs					613:617	diabetic HAECs	604:617	diabetic HAECs	604:617	METHODS ECs isolated from healthy individuals [human aortic ECs (HAECs)] and diabetic patients (diabetic HAECs) were treated with ±BBM (benzoic acid-4-sulfate, hippuric acid, hydroxyhippuric acid, isovanillic acid-3-sulfate, and vanillic acid-4-sulfate at concentrations known to circulate in human plasma following blueberry consumption) for 3 days, and indices for endothelial inflammation were measured.
29544868	3	60	theme	diabetic	604:611	arg1	METHODS					508:514	METHODS	508:514	METHODS ECs isolated from healthy individuals [human aortic ECs (HAECs)] and diabetic patients (diabetic HAECs)	508:618	METHODS ECs isolated from healthy individuals [human aortic ECs (HAECs)] and diabetic patients (diabetic HAECs) were treated with ±BBM (benzoic acid-4-sulfate, hippuric acid, hydroxyhippuric acid, isovanillic acid-3-sulfate, and vanillic acid-4-sulfate at concentrations known to circulate in human plasma following blueberry consumption) for 3 days, and indices for endothelial inflammation were measured.
29544868	0	61	from	inflammation	88:99	arg1	cells					138:142	diabetic human aortic endothelial cells	104:142	diabetic human aortic endothelial cells	104:142	Blueberry metabolites restore cell surface glycosaminoglycans and attenuate endothelial inflammation in diabetic human aortic endothelial cells.
29544868	3	62	theme	human	801:805	arg1	plasma					807:812	human plasma	801:812	human plasma	801:812	METHODS ECs isolated from healthy individuals [human aortic ECs (HAECs)] and diabetic patients (diabetic HAECs) were treated with ±BBM (benzoic acid-4-sulfate, hippuric acid, hydroxyhippuric acid, isovanillic acid-3-sulfate, and vanillic acid-4-sulfate at concentrations known to circulate in human plasma following blueberry consumption) for 3 days, and indices for endothelial inflammation were measured.
29544868	5	63	theme	high-pressure	1108:1120	arg1	chromatography					1129:1142	high-pressure liquid chromatography	1108:1142	high-pressure liquid chromatography coupled to an inline flow scintillation analyzer	1108:1191	Total GAGs in ECs and medium were purified using DEAE-Sepharose column and were analyzed with high-pressure liquid chromatography coupled to an inline flow scintillation analyzer.
29544868	6	64	from	medium	1282:1287	arg1	ratio					1230:1234	Heparan sulfate/chondroitin sulfate ratio	1194:1234	Heparan sulfate/chondroitin sulfate ratio	1194:1234	Heparan sulfate/chondroitin sulfate ratio and disaccharide composition of GAGs from the medium were analyzed using DEAE-3SW column and Dionex CarboPac PA1 column, respectively.
29544868	6	64	from	medium	1282:1287	arg1	composition					1253:1263	disaccharide composition	1240:1263	disaccharide composition	1240:1263	Heparan sulfate/chondroitin sulfate ratio and disaccharide composition of GAGs from the medium were analyzed using DEAE-3SW column and Dionex CarboPac PA1 column, respectively.
29544868	5	65	theme	DEAE-Sepharose	1063:1076	arg1	column					1078:1083	DEAE-Sepharose column	1063:1083	DEAE-Sepharose column	1063:1083	Total GAGs in ECs and medium were purified using DEAE-Sepharose column and were analyzed with high-pressure liquid chromatography coupled to an inline flow scintillation analyzer.
29544868	3	66	theme	endothelial	875:885	arg1	inflammation					887:898	endothelial inflammation	875:898	endothelial inflammation	875:898	METHODS ECs isolated from healthy individuals [human aortic ECs (HAECs)] and diabetic patients (diabetic HAECs) were treated with ±BBM (benzoic acid-4-sulfate, hippuric acid, hydroxyhippuric acid, isovanillic acid-3-sulfate, and vanillic acid-4-sulfate at concentrations known to circulate in human plasma following blueberry consumption) for 3 days, and indices for endothelial inflammation were measured.
29544868	8	67	theme	sulfate	1551:1557	arg1	incorporation					1559:1571	[35S] sulfate incorporation	1545:1571	[35S] sulfate incorporation into the cell surface GAGs indicating the dysregulation of sulfated GAGs	1545:1644	Diabetic HAECs displayed a decrease in [35S] sulfate incorporation into the cell surface GAGs indicating the dysregulation of sulfated GAGs.
29544868	6	68	theme	GAGs	1268:1271	arg1	ratio					1230:1234	Heparan sulfate/chondroitin sulfate ratio	1194:1234	Heparan sulfate/chondroitin sulfate ratio	1194:1234	Heparan sulfate/chondroitin sulfate ratio and disaccharide composition of GAGs from the medium were analyzed using DEAE-3SW column and Dionex CarboPac PA1 column, respectively.
29544868	6	68	theme	GAGs	1268:1271	arg1	composition					1253:1263	disaccharide composition	1240:1263	disaccharide composition	1240:1263	Heparan sulfate/chondroitin sulfate ratio and disaccharide composition of GAGs from the medium were analyzed using DEAE-3SW column and Dionex CarboPac PA1 column, respectively.
29544868	8	69	from	decrease	1533:1540	arg1	incorporation					1559:1571	[35S] sulfate incorporation	1545:1571	[35S] sulfate incorporation into the cell surface GAGs indicating the dysregulation of sulfated GAGs	1545:1644	Diabetic HAECs displayed a decrease in [35S] sulfate incorporation into the cell surface GAGs indicating the dysregulation of sulfated GAGs.
29544868	12	70	from	complications	2030:2042	arg1	diabetes					2047:2054	diabetes	2047:2054	diabetes	2047:2054	Blueberry might complement conventional therapies to improve vascular complications in diabetes.
29544868	0	71	theme	diabetic	104:111	arg1	cells					138:142	diabetic human aortic endothelial cells	104:142	diabetic human aortic endothelial cells	104:142	Blueberry metabolites restore cell surface glycosaminoglycans and attenuate endothelial inflammation in diabetic human aortic endothelial cells.
29544868	5	72	theme	inline	1158:1163	arg1	analyzer					1184:1191	an inline flow scintillation analyzer	1155:1191	an inline flow scintillation analyzer	1155:1191	Total GAGs in ECs and medium were purified using DEAE-Sepharose column and were analyzed with high-pressure liquid chromatography coupled to an inline flow scintillation analyzer.
29544868	8	73	theme	cell	1582:1585	arg1	GAGs					1595:1598	the cell surface GAGs	1578:1598	the cell surface GAGs indicating the dysregulation of sulfated GAGs	1578:1644	Diabetic HAECs displayed a decrease in [35S] sulfate incorporation into the cell surface GAGs indicating the dysregulation of sulfated GAGs.
29544868	11	74	theme	CONCLUSIONS	1858:1868	arg1	BBM					1870:1872	CONCLUSIONS BBM	1858:1872	CONCLUSIONS BBM	1858:1872	CONCLUSIONS BBM restored cell surface GAGs and attenuated endothelial inflammation in diabetic HAECs.
29544868	0	75	theme	aortic	119:124	arg1	cells					138:142	diabetic human aortic endothelial cells	104:142	diabetic human aortic endothelial cells	104:142	Blueberry metabolites restore cell surface glycosaminoglycans and attenuate endothelial inflammation in diabetic human aortic endothelial cells.
29544868	5	76	theme	scintillation	1170:1182	arg1	analyzer					1184:1191	an inline flow scintillation analyzer	1155:1191	an inline flow scintillation analyzer	1155:1191	Total GAGs in ECs and medium were purified using DEAE-Sepharose column and were analyzed with high-pressure liquid chromatography coupled to an inline flow scintillation analyzer.
29544868	4	77	theme	sulfate-free	956:967	arg1	medium					969:974	sulfate-free medium	956:974	sulfate-free medium supplemented with [35S] Na2SO4 ± BBM	956:1011	To analyze GAGs, ECs were incubated with sulfate-free medium supplemented with [35S] Na2SO4 ± BBM.
29544868	7	78	theme	diabetes-induced	1394:1409	arg1	binding					1420:1426	diabetes-induced monocyte binding	1394:1426	diabetes-induced monocyte binding to ECs	1394:1433	RESULTS BBM suppressed diabetes-induced monocyte binding to ECs, and reduced the expression of inflammatory markers in diabetic HAECs.
29544868	11	79	from	inflammation	1928:1939	arg1	HAECs					1953:1957	diabetic HAECs	1944:1957	diabetic HAECs	1944:1957	CONCLUSIONS BBM restored cell surface GAGs and attenuated endothelial inflammation in diabetic HAECs.
29544868	2	80	theme	endothelial	415:425	arg1	inflammation					427:438	endothelial inflammation	415:438	endothelial inflammation in diabetic endothelial cells (ECs)	415:474	We tested the hypothesis that blueberry metabolites (BBM) ameliorate endothelial inflammation in diabetic endothelial cells (ECs) by restoring cell surface GAGs.
29544868	0	81	theme	cell	30:33	arg1	glycosaminoglycans					43:60	cell surface glycosaminoglycans	30:60	cell surface glycosaminoglycans	30:60	Blueberry metabolites restore cell surface glycosaminoglycans and attenuate endothelial inflammation in diabetic human aortic endothelial cells.
29544868	8	82	theme	GAGs	1641:1644	arg1	dysregulation					1615:1627	the dysregulation	1611:1627	the dysregulation of sulfated GAGs	1611:1644	Diabetic HAECs displayed a decrease in [35S] sulfate incorporation into the cell surface GAGs indicating the dysregulation of sulfated GAGs.
29544868	3	83	theme	benzoic	644:650	arg1	acid-4-sulfate					652:665	benzoic acid-4-sulfate	644:665	benzoic acid-4-sulfate	644:665	METHODS ECs isolated from healthy individuals [human aortic ECs (HAECs)] and diabetic patients (diabetic HAECs) were treated with ±BBM (benzoic acid-4-sulfate, hippuric acid, hydroxyhippuric acid, isovanillic acid-3-sulfate, and vanillic acid-4-sulfate at concentrations known to circulate in human plasma following blueberry consumption) for 3 days, and indices for endothelial inflammation were measured.
29544868	10	84	theme	heparan	1739:1745	arg1	sulfate					1767:1773	heparan sulfate/chondroitin sulfate	1739:1773	heparan sulfate/chondroitin sulfate ratio	1739:1779	The composition, heparan sulfate/chondroitin sulfate ratio, and disaccharide composition of GAGs from medium were similar among groups.
29544868	7	85	from	expression	1452:1461	arg1	HAECs					1499:1503	diabetic HAECs	1490:1503	diabetic HAECs	1490:1503	RESULTS BBM suppressed diabetes-induced monocyte binding to ECs, and reduced the expression of inflammatory markers in diabetic HAECs.
29544868	2	86	theme	surface	494:500	arg1	GAGs					502:505	cell surface GAGs	489:505	cell surface GAGs	489:505	We tested the hypothesis that blueberry metabolites (BBM) ameliorate endothelial inflammation in diabetic endothelial cells (ECs) by restoring cell surface GAGs.
29544868	11	87	theme	endothelial	1916:1926	arg1	inflammation					1928:1939	endothelial inflammation	1916:1939	endothelial inflammation in diabetic HAECs	1916:1957	CONCLUSIONS BBM restored cell surface GAGs and attenuated endothelial inflammation in diabetic HAECs.
29544868	3	88	theme	healthy	534:540	arg1	individuals					542:552	healthy individuals	534:552	healthy individuals	534:552	METHODS ECs isolated from healthy individuals [human aortic ECs (HAECs)] and diabetic patients (diabetic HAECs) were treated with ±BBM (benzoic acid-4-sulfate, hippuric acid, hydroxyhippuric acid, isovanillic acid-3-sulfate, and vanillic acid-4-sulfate at concentrations known to circulate in human plasma following blueberry consumption) for 3 days, and indices for endothelial inflammation were measured.
29544868	5	89	theme	Total	1014:1018	arg1	GAGs					1020:1023	Total GAGs	1014:1023	Total GAGs in ECs and medium	1014:1041	Total GAGs in ECs and medium were purified using DEAE-Sepharose column and were analyzed with high-pressure liquid chromatography coupled to an inline flow scintillation analyzer.
29544868	10	90	theme	sulfate	1767:1773	arg1	ratio					1775:1779	heparan sulfate/chondroitin sulfate ratio	1739:1779	heparan sulfate/chondroitin sulfate ratio	1739:1779	The composition, heparan sulfate/chondroitin sulfate ratio, and disaccharide composition of GAGs from medium were similar among groups.
29544868	9	91	theme	GAGs	1698:1701	arg1	levels					1688:1693	the levels	1684:1693	the levels of GAGs in diabetic HAECs	1684:1719	However, treatment with BBM restored the levels of GAGs in diabetic HAECs.
29544868	1	92	theme	diabetic	255:262	arg1	vasculature					264:274	diabetic vasculature	255:274	diabetic vasculature leading to endothelial inflammation and vascular disease in diabetes	255:343	BACKGROUND Glycosaminoglycan (GAG), a major component of the endothelial glycocalyx, is severely perturbed in diabetic vasculature leading to endothelial inflammation and vascular disease in diabetes.
29544868	3	93	theme	[human	554:559	arg1	HAECs					573:577	HAECs	573:577	HAECs	573:577	METHODS ECs isolated from healthy individuals [human aortic ECs (HAECs)] and diabetic patients (diabetic HAECs) were treated with ±BBM (benzoic acid-4-sulfate, hippuric acid, hydroxyhippuric acid, isovanillic acid-3-sulfate, and vanillic acid-4-sulfate at concentrations known to circulate in human plasma following blueberry consumption) for 3 days, and indices for endothelial inflammation were measured.
29544868	3	93	theme	[human	554:559	arg1	ECs					568:570	[human aortic ECs	554:570	[human aortic ECs (HAECs)]	554:579	METHODS ECs isolated from healthy individuals [human aortic ECs (HAECs)] and diabetic patients (diabetic HAECs) were treated with ±BBM (benzoic acid-4-sulfate, hippuric acid, hydroxyhippuric acid, isovanillic acid-3-sulfate, and vanillic acid-4-sulfate at concentrations known to circulate in human plasma following blueberry consumption) for 3 days, and indices for endothelial inflammation were measured.
29544868	3	94	dep	acid-4-sulfate	652:665	arg1	circulate					788:796	circulate	788:796	to circulate in human plasma following blueberry consumption	785:844	METHODS ECs isolated from healthy individuals [human aortic ECs (HAECs)] and diabetic patients (diabetic HAECs) were treated with ±BBM (benzoic acid-4-sulfate, hippuric acid, hydroxyhippuric acid, isovanillic acid-3-sulfate, and vanillic acid-4-sulfate at concentrations known to circulate in human plasma following blueberry consumption) for 3 days, and indices for endothelial inflammation were measured.
30101726	7	0	theme	significant	1066:1076	arg1	losses					1083:1088	significant milk losses	1066:1088	significant milk losses in multipara	1066:1101	All pathogens, particularly S. aureus and fungi, were associated with significant milk losses in multipara.
30101726	1	1	theme	urea	362:365	arg1	MUN					377:379	MUN	377:379	MUN	377:379	The aim of this study was to estimate the associations of the first occurrence of pathogen-specific clinical mastitis (CM) with milk yield and milk composition (somatic cell count (SCC), lactose, fat, protein content in milk and milk urea nitrogen (MUN)).
30101726	1	1	theme	urea	362:365	arg1	nitrogen					367:374	milk urea nitrogen	357:374	milk urea nitrogen (MUN)	357:380	The aim of this study was to estimate the associations of the first occurrence of pathogen-specific clinical mastitis (CM) with milk yield and milk composition (somatic cell count (SCC), lactose, fat, protein content in milk and milk urea nitrogen (MUN)).
30101726	8	2	from	loss	1190:1193	arg1	primipara					1198:1206	primipara	1198:1206	primipara	1198:1206	In this study, S. aureus and CNS infections were not associated with significant milk loss in primipara.
30101726	5	3	theme	CM	788:789	arg1	effects					777:783	the effects	773:783	the effects of CM and several other control variables (herd, calving season, parity, week of lactation, and other diseases)	773:895	Mixed models with an autoregressive correlation structure were fitted to quantify the effects of CM and several other control variables (herd, calving season, parity, week of lactation, and other diseases).
30101726	12	4	theme	Streptococcus	1538:1550	arg1	spp					1552:1554	Streptococcus spp	1538:1554	Streptococcus spp	1538:1554	Some pathogens such as Streptococcus spp.
30101726	14	5	theme	better	1743:1748	arg1	indicators					1750:1759	better indicators	1743:1759	better indicators of pathogen-specific mastitis	1743:1789	These findings provide estimates that could be used to calculate precise costs of CM, and also provide better indicators of pathogen-specific mastitis.
30101726	0	6	theme	dairy	116:120	arg1	cows					122:125	dairy cows	116:125	dairy cows	116:125	Associations of the first occurrence of pathogen-specific clinical mastitis with milk yield and milk composition in dairy cows.
30101726	3	7	theme	pathogen	453:460	arg1	groups					462:467	Five pathogen groups	448:467	Five pathogen groups	448:467	Five pathogen groups were studied: Streptococcus spp.; Staphylococcus aureus (S. aureus); coagulase-negative staphylococci (CNS); coliforms; and fungi.
30101726	10	8	dep	aureus	1362:1367	arg1	multipara					1373:1381	in multipara	1370:1381	in multipara	1370:1381	All pathogens, especially CNS (in primipara) and S. aureus (in multipara), decreased lactose content.
30101726	1	9	theme	protein	329:335	arg1	content					337:343	protein content	329:343	protein content in milk	329:351	The aim of this study was to estimate the associations of the first occurrence of pathogen-specific clinical mastitis (CM) with milk yield and milk composition (somatic cell count (SCC), lactose, fat, protein content in milk and milk urea nitrogen (MUN)).
30101726	5	10	theme	Mixed	691:695	arg1	models					697:702	Mixed models	691:702	Mixed models with an autoregressive correlation structure	691:747	Mixed models with an autoregressive correlation structure were fitted to quantify the effects of CM and several other control variables (herd, calving season, parity, week of lactation, and other diseases).
30101726	5	11	theme	correlation	727:737	arg1	structure					739:747	an autoregressive correlation structure	709:747	an autoregressive correlation structure	709:747	Mixed models with an autoregressive correlation structure were fitted to quantify the effects of CM and several other control variables (herd, calving season, parity, week of lactation, and other diseases).
30101726	8	12	theme	milk	1185:1188	arg1	loss					1190:1193	significant milk loss	1173:1193	significant milk loss in primipara	1173:1206	In this study, S. aureus and CNS infections were not associated with significant milk loss in primipara.
30101726	0	13	from	composition	101:111	arg1	cows					122:125	dairy cows	116:125	dairy cows	116:125	Associations of the first occurrence of pathogen-specific clinical mastitis with milk yield and milk composition in dairy cows.
30101726	8	14	theme	aureus	1122:1127	arg1	infections					1137:1146	S. aureus and CNS infections	1119:1146	S. aureus and CNS infections	1119:1146	In this study, S. aureus and CNS infections were not associated with significant milk loss in primipara.
30101726	5	15	theme	other	803:807	arg1	week					858:861	week	858:861	week of lactation	858:874	Mixed models with an autoregressive correlation structure were fitted to quantify the effects of CM and several other control variables (herd, calving season, parity, week of lactation, and other diseases).
30101726	5	15	theme	other	803:807	arg1	parity					850:855	parity	850:855	parity	850:855	Mixed models with an autoregressive correlation structure were fitted to quantify the effects of CM and several other control variables (herd, calving season, parity, week of lactation, and other diseases).
30101726	5	15	theme	other	803:807	arg1	herd					828:831	herd	828:831	herd	828:831	Mixed models with an autoregressive correlation structure were fitted to quantify the effects of CM and several other control variables (herd, calving season, parity, week of lactation, and other diseases).
30101726	5	15	theme	other	803:807	arg1	season					842:847	calving season	834:847	calving season	834:847	Mixed models with an autoregressive correlation structure were fitted to quantify the effects of CM and several other control variables (herd, calving season, parity, week of lactation, and other diseases).
30101726	5	15	theme	other	803:807	arg1	variables					817:825	several other control variables	795:825	several other control variables (herd, calving season, parity, week of lactation, and other diseases)	795:895	Mixed models with an autoregressive correlation structure were fitted to quantify the effects of CM and several other control variables (herd, calving season, parity, week of lactation, and other diseases).
30101726	4	16	theme	Test-day	600:607	arg1	data					614:617	Test-day milk data	600:617	Test-day milk data	600:617	Test-day milk data and clinical records were collected from June 2011 until February 2014.
30101726	8	17	theme	CNS	1133:1135	arg1	infections					1137:1146	S. aureus and CNS infections	1119:1146	S. aureus and CNS infections	1119:1146	In this study, S. aureus and CNS infections were not associated with significant milk loss in primipara.
30101726	1	18	from	nitrogen	367:374	arg1	milk					348:351	milk	348:351	milk	348:351	The aim of this study was to estimate the associations of the first occurrence of pathogen-specific clinical mastitis (CM) with milk yield and milk composition (somatic cell count (SCC), lactose, fat, protein content in milk and milk urea nitrogen (MUN)).
30101726	1	19	theme	cell	297:300	arg1	SCC					309:311	SCC	309:311	SCC	309:311	The aim of this study was to estimate the associations of the first occurrence of pathogen-specific clinical mastitis (CM) with milk yield and milk composition (somatic cell count (SCC), lactose, fat, protein content in milk and milk urea nitrogen (MUN)).
30101726	1	19	theme	cell	297:300	arg1	count					302:306	somatic cell count	289:306	somatic cell count (SCC)	289:312	The aim of this study was to estimate the associations of the first occurrence of pathogen-specific clinical mastitis (CM) with milk yield and milk composition (somatic cell count (SCC), lactose, fat, protein content in milk and milk urea nitrogen (MUN)).
30101726	0	20	theme	milk	81:84	arg1	yield					86:90	milk yield	81:90	milk yield	81:90	Associations of the first occurrence of pathogen-specific clinical mastitis with milk yield and milk composition in dairy cows.
30101726	1	21	theme	study	144:148	arg1	aim					132:134	The aim	128:134	The aim of this study	128:148	The aim of this study was to estimate the associations of the first occurrence of pathogen-specific clinical mastitis (CM) with milk yield and milk composition (somatic cell count (SCC), lactose, fat, protein content in milk and milk urea nitrogen (MUN)).
30101726	2	22	theme	dairy	426:430	arg1	farms					432:436	31 Hokkaido dairy farms	414:436	31 Hokkaido dairy farms in Japan	414:445	We studied 3149 dairy cows in 31 Hokkaido dairy farms in Japan.
30101726	1	23	from	content	337:343	arg1	milk					348:351	milk	348:351	milk	348:351	The aim of this study was to estimate the associations of the first occurrence of pathogen-specific clinical mastitis (CM) with milk yield and milk composition (somatic cell count (SCC), lactose, fat, protein content in milk and milk urea nitrogen (MUN)).
30101726	14	24	theme	mastitis	1782:1789	arg1	indicators					1750:1759	better indicators	1743:1759	better indicators of pathogen-specific mastitis	1743:1789	These findings provide estimates that could be used to calculate precise costs of CM, and also provide better indicators of pathogen-specific mastitis.
30101726	11	25	from	changes	1482:1488	arg1	MUN					1510:1512	MUN	1510:1512	MUN	1510:1512	All pathogen groups except for fungi were associated with significant changes in fat, protein and MUN.
30101726	11	25	from	changes	1482:1488	arg1	protein					1498:1504	protein	1498:1504	protein	1498:1504	All pathogen groups except for fungi were associated with significant changes in fat, protein and MUN.
30101726	11	25	from	changes	1482:1488	arg1	fat					1493:1495	fat	1493:1495	fat	1493:1495	All pathogen groups except for fungi were associated with significant changes in fat, protein and MUN.
30101726	8	26	theme	significant	1173:1183	arg1	loss					1190:1193	significant milk loss	1173:1193	significant milk loss in primipara	1173:1206	In this study, S. aureus and CNS infections were not associated with significant milk loss in primipara.
30101726	13	27	theme	MUN	1627:1629	arg1	changes					1631:1637	long-term fat, protein and MUN changes	1600:1637	long-term fat, protein and MUN changes	1600:1637	and coliforms seemed to be associated with long-term fat, protein and MUN changes.
30101726	5	28	theme	variables	817:825	arg1	effects					777:783	the effects	773:783	the effects of CM and several other control variables (herd, calving season, parity, week of lactation, and other diseases)	773:895	Mixed models with an autoregressive correlation structure were fitted to quantify the effects of CM and several other control variables (herd, calving season, parity, week of lactation, and other diseases).
30101726	4	29	theme	clinical	623:630	arg1	records					632:638	clinical records	623:638	clinical records	623:638	Test-day milk data and clinical records were collected from June 2011 until February 2014.
30101726	13	30	theme	fat	1610:1612	arg1	changes					1631:1637	long-term fat, protein and MUN changes	1600:1637	long-term fat, protein and MUN changes	1600:1637	and coliforms seemed to be associated with long-term fat, protein and MUN changes.
30101726	13	31	theme	protein	1615:1621	arg1	changes					1631:1637	long-term fat, protein and MUN changes	1600:1637	long-term fat, protein and MUN changes	1600:1637	and coliforms seemed to be associated with long-term fat, protein and MUN changes.
30101726	14	32	used	used	1687:1690	arg2	estimates					1663:1671	estimates	1663:1671	estimates that could be used to calculate precise costs of CM, and also provide better indicators of pathogen-specific mastitis	1663:1789	These findings provide estimates that could be used to calculate precise costs of CM, and also provide better indicators of pathogen-specific mastitis.
30101726	0	33	theme	occurrence	26:35	arg1	Associations					0:11	Associations	0:11	Associations of the first occurrence of pathogen-specific clinical mastitis with milk yield and milk composition in dairy cows.	0:126	Associations of the first occurrence of pathogen-specific clinical mastitis with milk yield and milk composition in dairy cows.
30101726	1	34	theme	milk	271:274	arg1	composition					276:286	milk composition	271:286	milk composition	271:286	The aim of this study was to estimate the associations of the first occurrence of pathogen-specific clinical mastitis (CM) with milk yield and milk composition (somatic cell count (SCC), lactose, fat, protein content in milk and milk urea nitrogen (MUN)).
30101726	0	35	theme	mastitis	67:74	arg1	occurrence					26:35	the first occurrence	16:35	the first occurrence of pathogen-specific clinical mastitis	16:74	Associations of the first occurrence of pathogen-specific clinical mastitis with milk yield and milk composition in dairy cows.
30101726	1	36	from	count	302:306	arg1	milk					348:351	milk	348:351	milk	348:351	The aim of this study was to estimate the associations of the first occurrence of pathogen-specific clinical mastitis (CM) with milk yield and milk composition (somatic cell count (SCC), lactose, fat, protein content in milk and milk urea nitrogen (MUN)).
30101726	5	37	theme	other	881:885	arg1	diseases					887:894	other diseases	881:894	other diseases	881:894	Mixed models with an autoregressive correlation structure were fitted to quantify the effects of CM and several other control variables (herd, calving season, parity, week of lactation, and other diseases).
30101726	0	38	with	Associations	0:11	arg1	yield					86:90	milk yield	81:90	milk yield	81:90	Associations of the first occurrence of pathogen-specific clinical mastitis with milk yield and milk composition in dairy cows.
30101726	0	38	with	Associations	0:11	arg1	composition					101:111	milk composition	96:111	milk composition	96:111	Associations of the first occurrence of pathogen-specific clinical mastitis with milk yield and milk composition in dairy cows.
30101726	1	39	theme	occurrence	196:205	arg1	associations					170:181	the associations	166:181	the associations of the first occurrence of pathogen-specific clinical mastitis (CM) with milk yield and milk composition (somatic cell count (SCC), lactose, fat, protein content in milk and milk urea nitrogen (MUN))	166:381	The aim of this study was to estimate the associations of the first occurrence of pathogen-specific clinical mastitis (CM) with milk yield and milk composition (somatic cell count (SCC), lactose, fat, protein content in milk and milk urea nitrogen (MUN)).
30101726	14	40	theme	precise	1705:1711	arg1	costs					1713:1717	precise costs	1705:1717	precise costs of CM	1705:1723	These findings provide estimates that could be used to calculate precise costs of CM, and also provide better indicators of pathogen-specific mastitis.
30101726	2	41	from	farms	432:436	arg1	Japan					441:445	Japan	441:445	Japan	441:445	We studied 3149 dairy cows in 31 Hokkaido dairy farms in Japan.
30101726	1	42	theme	pathogen-specific	210:226	arg1	CM					247:248	CM	247:248	CM	247:248	The aim of this study was to estimate the associations of the first occurrence of pathogen-specific clinical mastitis (CM) with milk yield and milk composition (somatic cell count (SCC), lactose, fat, protein content in milk and milk urea nitrogen (MUN)).
30101726	1	42	theme	pathogen-specific	210:226	arg1	mastitis					237:244	pathogen-specific clinical mastitis	210:244	pathogen-specific clinical mastitis (CM)	210:249	The aim of this study was to estimate the associations of the first occurrence of pathogen-specific clinical mastitis (CM) with milk yield and milk composition (somatic cell count (SCC), lactose, fat, protein content in milk and milk urea nitrogen (MUN)).
30101726	11	43	theme	significant	1470:1480	arg1	changes					1482:1488	significant changes	1470:1488	significant changes in fat, protein and MUN	1470:1512	All pathogen groups except for fungi were associated with significant changes in fat, protein and MUN.
30101726	1	44	theme	mastitis	237:244	arg1	occurrence					196:205	the first occurrence	186:205	the first occurrence of pathogen-specific clinical mastitis (CM)	186:249	The aim of this study was to estimate the associations of the first occurrence of pathogen-specific clinical mastitis (CM) with milk yield and milk composition (somatic cell count (SCC), lactose, fat, protein content in milk and milk urea nitrogen (MUN)).
30101726	7	45	theme	milk	1078:1081	arg1	losses					1083:1088	significant milk losses	1066:1088	significant milk losses in multipara	1066:1101	All pathogens, particularly S. aureus and fungi, were associated with significant milk losses in multipara.
30101726	11	46	theme	pathogen	1416:1423	arg1	groups					1425:1430	All pathogen groups	1412:1430	All pathogen groups except for fungi	1412:1447	All pathogen groups except for fungi were associated with significant changes in fat, protein and MUN.
30101726	7	47	from	losses	1083:1088	arg1	multipara					1093:1101	multipara	1093:1101	multipara	1093:1101	All pathogens, particularly S. aureus and fungi, were associated with significant milk losses in multipara.
30101726	3	48	dep	studied	474:480	arg1	Streptococcus					483:495	Streptococcus	483:495	Streptococcus	483:495	Five pathogen groups were studied: Streptococcus spp.; Staphylococcus aureus (S. aureus); coagulase-negative staphylococci (CNS); coliforms; and fungi.
30101726	0	49	theme	pathogen-specific	40:56	arg1	mastitis					67:74	pathogen-specific clinical mastitis	40:74	pathogen-specific clinical mastitis	40:74	Associations of the first occurrence of pathogen-specific clinical mastitis with milk yield and milk composition in dairy cows.
30101726	3	50	dep	Streptococcus	483:495	arg1	fungi					593:597	fungi	593:597	fungi	593:597	Five pathogen groups were studied: Streptococcus spp.; Staphylococcus aureus (S. aureus); coagulase-negative staphylococci (CNS); coliforms; and fungi.
30101726	3	50	dep	Streptococcus	483:495	arg1	spp.					497:500	Streptococcus spp.	483:500	Streptococcus spp.; Staphylococcus aureus (S. aureus); coagulase-negative staphylococci (CNS); coliforms; and fungi	483:597	Five pathogen groups were studied: Streptococcus spp.; Staphylococcus aureus (S. aureus); coagulase-negative staphylococci (CNS); coliforms; and fungi.
30101726	3	50	dep	Streptococcus	483:495	arg1	coliforms					578:586	coliforms	578:586	coliforms	578:586	Five pathogen groups were studied: Streptococcus spp.; Staphylococcus aureus (S. aureus); coagulase-negative staphylococci (CNS); coliforms; and fungi.
30101726	3	50	dep	Streptococcus	483:495	arg1	aureus					518:523	Staphylococcus aureus	503:523	Staphylococcus aureus (S. aureus)	503:535	Five pathogen groups were studied: Streptococcus spp.; Staphylococcus aureus (S. aureus); coagulase-negative staphylococci (CNS); coliforms; and fungi.
30101726	3	50	dep	Streptococcus	483:495	arg1	staphylococci					557:569	coagulase-negative staphylococci	538:569	coagulase-negative staphylococci (CNS)	538:575	Five pathogen groups were studied: Streptococcus spp.; Staphylococcus aureus (S. aureus); coagulase-negative staphylococci (CNS); coliforms; and fungi.
30101726	3	50	dep	Streptococcus	483:495	arg1	CNS					572:574	CNS	572:574	CNS	572:574	Five pathogen groups were studied: Streptococcus spp.; Staphylococcus aureus (S. aureus); coagulase-negative staphylococci (CNS); coliforms; and fungi.
30101726	5	51	with	models	697:702	arg1	structure					739:747	an autoregressive correlation structure	709:747	an autoregressive correlation structure	709:747	Mixed models with an autoregressive correlation structure were fitted to quantify the effects of CM and several other control variables (herd, calving season, parity, week of lactation, and other diseases).
30101726	0	52	from	yield	86:90	arg1	cows					122:125	dairy cows	116:125	dairy cows	116:125	Associations of the first occurrence of pathogen-specific clinical mastitis with milk yield and milk composition in dairy cows.
30101726	5	53	theme	autoregressive	712:725	arg1	structure					739:747	an autoregressive correlation structure	709:747	an autoregressive correlation structure	709:747	Mixed models with an autoregressive correlation structure were fitted to quantify the effects of CM and several other control variables (herd, calving season, parity, week of lactation, and other diseases).
30101726	10	54	theme	lactose	1395:1401	arg1	content					1403:1409	lactose content	1395:1409	lactose content	1395:1409	All pathogens, especially CNS (in primipara) and S. aureus (in multipara), decreased lactose content.
30101726	9	55	theme	parity	1295:1300	arg1	groups					1302:1307	both parity groups	1290:1307	both parity groups	1290:1307	All pathogens, in particular S. aureus and fungi, significantly increased SCC in both parity groups.
30101726	5	56	theme	several	795:801	arg1	week					858:861	week	858:861	week of lactation	858:874	Mixed models with an autoregressive correlation structure were fitted to quantify the effects of CM and several other control variables (herd, calving season, parity, week of lactation, and other diseases).
30101726	5	56	theme	several	795:801	arg1	parity					850:855	parity	850:855	parity	850:855	Mixed models with an autoregressive correlation structure were fitted to quantify the effects of CM and several other control variables (herd, calving season, parity, week of lactation, and other diseases).
30101726	5	56	theme	several	795:801	arg1	herd					828:831	herd	828:831	herd	828:831	Mixed models with an autoregressive correlation structure were fitted to quantify the effects of CM and several other control variables (herd, calving season, parity, week of lactation, and other diseases).
30101726	5	56	theme	several	795:801	arg1	season					842:847	calving season	834:847	calving season	834:847	Mixed models with an autoregressive correlation structure were fitted to quantify the effects of CM and several other control variables (herd, calving season, parity, week of lactation, and other diseases).
30101726	5	56	theme	several	795:801	arg1	variables					817:825	several other control variables	795:825	several other control variables (herd, calving season, parity, week of lactation, and other diseases)	795:895	Mixed models with an autoregressive correlation structure were fitted to quantify the effects of CM and several other control variables (herd, calving season, parity, week of lactation, and other diseases).
30101726	1	57	theme	milk	357:360	arg1	MUN					377:379	MUN	377:379	MUN	377:379	The aim of this study was to estimate the associations of the first occurrence of pathogen-specific clinical mastitis (CM) with milk yield and milk composition (somatic cell count (SCC), lactose, fat, protein content in milk and milk urea nitrogen (MUN)).
30101726	1	57	theme	milk	357:360	arg1	nitrogen					367:374	milk urea nitrogen	357:374	milk urea nitrogen (MUN)	357:380	The aim of this study was to estimate the associations of the first occurrence of pathogen-specific clinical mastitis (CM) with milk yield and milk composition (somatic cell count (SCC), lactose, fat, protein content in milk and milk urea nitrogen (MUN)).
30101726	2	58	theme	dairy	400:404	arg1	cows					406:409	3149 dairy cows	395:409	3149 dairy cows	395:409	We studied 3149 dairy cows in 31 Hokkaido dairy farms in Japan.
30101726	1	59	dep	yield	261:265	arg1	content					337:343	protein content	329:343	protein content in milk	329:351	The aim of this study was to estimate the associations of the first occurrence of pathogen-specific clinical mastitis (CM) with milk yield and milk composition (somatic cell count (SCC), lactose, fat, protein content in milk and milk urea nitrogen (MUN)).
30101726	1	59	dep	yield	261:265	arg1	nitrogen					367:374	milk urea nitrogen	357:374	milk urea nitrogen (MUN)	357:380	The aim of this study was to estimate the associations of the first occurrence of pathogen-specific clinical mastitis (CM) with milk yield and milk composition (somatic cell count (SCC), lactose, fat, protein content in milk and milk urea nitrogen (MUN)).
30101726	1	59	dep	yield	261:265	arg1	MUN					377:379	MUN	377:379	MUN	377:379	The aim of this study was to estimate the associations of the first occurrence of pathogen-specific clinical mastitis (CM) with milk yield and milk composition (somatic cell count (SCC), lactose, fat, protein content in milk and milk urea nitrogen (MUN)).
30101726	1	59	dep	yield	261:265	arg1	lactose					315:321	lactose	315:321	lactose	315:321	The aim of this study was to estimate the associations of the first occurrence of pathogen-specific clinical mastitis (CM) with milk yield and milk composition (somatic cell count (SCC), lactose, fat, protein content in milk and milk urea nitrogen (MUN)).
30101726	1	59	dep	yield	261:265	arg1	count					302:306	somatic cell count	289:306	somatic cell count (SCC)	289:312	The aim of this study was to estimate the associations of the first occurrence of pathogen-specific clinical mastitis (CM) with milk yield and milk composition (somatic cell count (SCC), lactose, fat, protein content in milk and milk urea nitrogen (MUN)).
30101726	1	59	dep	yield	261:265	arg1	fat					324:326	fat	324:326	fat	324:326	The aim of this study was to estimate the associations of the first occurrence of pathogen-specific clinical mastitis (CM) with milk yield and milk composition (somatic cell count (SCC), lactose, fat, protein content in milk and milk urea nitrogen (MUN)).
30101726	1	59	dep	yield	261:265	arg1	SCC					309:311	SCC	309:311	SCC	309:311	The aim of this study was to estimate the associations of the first occurrence of pathogen-specific clinical mastitis (CM) with milk yield and milk composition (somatic cell count (SCC), lactose, fat, protein content in milk and milk urea nitrogen (MUN)).
30101726	10	60	dep	CNS	1336:1338	arg1	primipara					1344:1352	in primipara	1341:1352	in primipara	1341:1352	All pathogens, especially CNS (in primipara) and S. aureus (in multipara), decreased lactose content.
30101726	5	61	dep	variables	817:825	arg1	week					858:861	week	858:861	week of lactation	858:874	Mixed models with an autoregressive correlation structure were fitted to quantify the effects of CM and several other control variables (herd, calving season, parity, week of lactation, and other diseases).
30101726	5	61	dep	variables	817:825	arg1	diseases					887:894	other diseases	881:894	other diseases	881:894	Mixed models with an autoregressive correlation structure were fitted to quantify the effects of CM and several other control variables (herd, calving season, parity, week of lactation, and other diseases).
30101726	5	61	dep	variables	817:825	arg1	season					842:847	calving season	834:847	calving season	834:847	Mixed models with an autoregressive correlation structure were fitted to quantify the effects of CM and several other control variables (herd, calving season, parity, week of lactation, and other diseases).
30101726	5	61	dep	variables	817:825	arg1	variables					817:825	several other control variables	795:825	several other control variables (herd, calving season, parity, week of lactation, and other diseases)	795:895	Mixed models with an autoregressive correlation structure were fitted to quantify the effects of CM and several other control variables (herd, calving season, parity, week of lactation, and other diseases).
30101726	5	61	dep	variables	817:825	arg1	parity					850:855	parity	850:855	parity	850:855	Mixed models with an autoregressive correlation structure were fitted to quantify the effects of CM and several other control variables (herd, calving season, parity, week of lactation, and other diseases).
30101726	5	61	dep	variables	817:825	arg1	herd					828:831	herd	828:831	herd	828:831	Mixed models with an autoregressive correlation structure were fitted to quantify the effects of CM and several other control variables (herd, calving season, parity, week of lactation, and other diseases).
30101726	0	62	theme	milk	96:99	arg1	composition					101:111	milk composition	96:111	milk composition	96:111	Associations of the first occurrence of pathogen-specific clinical mastitis with milk yield and milk composition in dairy cows.
30101726	2	63	theme	Hokkaido	417:424	arg1	farms					432:436	31 Hokkaido dairy farms	414:436	31 Hokkaido dairy farms in Japan	414:445	We studied 3149 dairy cows in 31 Hokkaido dairy farms in Japan.
30101726	5	64	theme	calving	834:840	arg1	season					842:847	calving season	834:847	calving season	834:847	Mixed models with an autoregressive correlation structure were fitted to quantify the effects of CM and several other control variables (herd, calving season, parity, week of lactation, and other diseases).
30101726	5	64	theme	calving	834:840	arg1	variables					817:825	several other control variables	795:825	several other control variables (herd, calving season, parity, week of lactation, and other diseases)	795:895	Mixed models with an autoregressive correlation structure were fitted to quantify the effects of CM and several other control variables (herd, calving season, parity, week of lactation, and other diseases).
30101726	1	65	from	fat	324:326	arg1	milk					348:351	milk	348:351	milk	348:351	The aim of this study was to estimate the associations of the first occurrence of pathogen-specific clinical mastitis (CM) with milk yield and milk composition (somatic cell count (SCC), lactose, fat, protein content in milk and milk urea nitrogen (MUN)).
30101726	14	66	theme	pathogen-specific	1764:1780	arg1	mastitis					1782:1789	pathogen-specific mastitis	1764:1789	pathogen-specific mastitis	1764:1789	These findings provide estimates that could be used to calculate precise costs of CM, and also provide better indicators of pathogen-specific mastitis.
30101726	1	67	from	lactose	315:321	arg1	milk					348:351	milk	348:351	milk	348:351	The aim of this study was to estimate the associations of the first occurrence of pathogen-specific clinical mastitis (CM) with milk yield and milk composition (somatic cell count (SCC), lactose, fat, protein content in milk and milk urea nitrogen (MUN)).
30101726	5	68	theme	control	809:815	arg1	week					858:861	week	858:861	week of lactation	858:874	Mixed models with an autoregressive correlation structure were fitted to quantify the effects of CM and several other control variables (herd, calving season, parity, week of lactation, and other diseases).
30101726	5	68	theme	control	809:815	arg1	parity					850:855	parity	850:855	parity	850:855	Mixed models with an autoregressive correlation structure were fitted to quantify the effects of CM and several other control variables (herd, calving season, parity, week of lactation, and other diseases).
30101726	5	68	theme	control	809:815	arg1	herd					828:831	herd	828:831	herd	828:831	Mixed models with an autoregressive correlation structure were fitted to quantify the effects of CM and several other control variables (herd, calving season, parity, week of lactation, and other diseases).
30101726	5	68	theme	control	809:815	arg1	season					842:847	calving season	834:847	calving season	834:847	Mixed models with an autoregressive correlation structure were fitted to quantify the effects of CM and several other control variables (herd, calving season, parity, week of lactation, and other diseases).
30101726	5	68	theme	control	809:815	arg1	variables					817:825	several other control variables	795:825	several other control variables (herd, calving season, parity, week of lactation, and other diseases)	795:895	Mixed models with an autoregressive correlation structure were fitted to quantify the effects of CM and several other control variables (herd, calving season, parity, week of lactation, and other diseases).
30101726	6	69	theme	first	909:913	arg1	Primipara					898:906	Primipara	898:906	Primipara (first lactation)	898:924	Primipara (first lactation) and multipara (second and later lactations) were analysed separately.
30101726	6	69	theme	first	909:913	arg1	lactation					915:923	first lactation	909:923	first lactation	909:923	Primipara (first lactation) and multipara (second and later lactations) were analysed separately.
30101726	4	70	theme	milk	609:612	arg1	data					614:617	Test-day milk data	600:617	Test-day milk data	600:617	Test-day milk data and clinical records were collected from June 2011 until February 2014.
30101726	13	71	theme	long-term	1600:1608	arg1	changes					1631:1637	long-term fat, protein and MUN changes	1600:1637	long-term fat, protein and MUN changes	1600:1637	and coliforms seemed to be associated with long-term fat, protein and MUN changes.
30101726	0	72	theme	first	20:24	arg1	occurrence					26:35	the first occurrence	16:35	the first occurrence of pathogen-specific clinical mastitis	16:74	Associations of the first occurrence of pathogen-specific clinical mastitis with milk yield and milk composition in dairy cows.
30101726	1	73	theme	milk	256:259	arg1	yield					261:265	milk yield	256:265	milk yield	256:265	The aim of this study was to estimate the associations of the first occurrence of pathogen-specific clinical mastitis (CM) with milk yield and milk composition (somatic cell count (SCC), lactose, fat, protein content in milk and milk urea nitrogen (MUN)).
30101726	1	74	with	associations	170:181	arg1	yield					261:265	milk yield	256:265	milk yield	256:265	The aim of this study was to estimate the associations of the first occurrence of pathogen-specific clinical mastitis (CM) with milk yield and milk composition (somatic cell count (SCC), lactose, fat, protein content in milk and milk urea nitrogen (MUN)).
30101726	1	74	with	associations	170:181	arg1	composition					276:286	milk composition	271:286	milk composition	271:286	The aim of this study was to estimate the associations of the first occurrence of pathogen-specific clinical mastitis (CM) with milk yield and milk composition (somatic cell count (SCC), lactose, fat, protein content in milk and milk urea nitrogen (MUN)).
30101726	3	75	dep	aureus	518:523	arg1	aureus					529:534	S. aureus	526:534	S. aureus	526:534	Five pathogen groups were studied: Streptococcus spp.; Staphylococcus aureus (S. aureus); coagulase-negative staphylococci (CNS); coliforms; and fungi.
30101726	5	76	theme	lactation	866:874	arg1	week					858:861	week	858:861	week of lactation	858:874	Mixed models with an autoregressive correlation structure were fitted to quantify the effects of CM and several other control variables (herd, calving season, parity, week of lactation, and other diseases).
30101726	5	76	theme	lactation	866:874	arg1	diseases					887:894	other diseases	881:894	other diseases	881:894	Mixed models with an autoregressive correlation structure were fitted to quantify the effects of CM and several other control variables (herd, calving season, parity, week of lactation, and other diseases).
30101726	5	76	theme	lactation	866:874	arg1	season					842:847	calving season	834:847	calving season	834:847	Mixed models with an autoregressive correlation structure were fitted to quantify the effects of CM and several other control variables (herd, calving season, parity, week of lactation, and other diseases).
30101726	5	76	theme	lactation	866:874	arg1	variables					817:825	several other control variables	795:825	several other control variables (herd, calving season, parity, week of lactation, and other diseases)	795:895	Mixed models with an autoregressive correlation structure were fitted to quantify the effects of CM and several other control variables (herd, calving season, parity, week of lactation, and other diseases).
30101726	5	76	theme	lactation	866:874	arg1	parity					850:855	parity	850:855	parity	850:855	Mixed models with an autoregressive correlation structure were fitted to quantify the effects of CM and several other control variables (herd, calving season, parity, week of lactation, and other diseases).
30101726	5	76	theme	lactation	866:874	arg1	herd					828:831	herd	828:831	herd	828:831	Mixed models with an autoregressive correlation structure were fitted to quantify the effects of CM and several other control variables (herd, calving season, parity, week of lactation, and other diseases).
30101726	0	77	theme	clinical	58:65	arg1	mastitis					67:74	pathogen-specific clinical mastitis	40:74	pathogen-specific clinical mastitis	40:74	Associations of the first occurrence of pathogen-specific clinical mastitis with milk yield and milk composition in dairy cows.
30101726	9	78	from	pathogens	1213:1221	arg1	fungi					1252:1256	fungi	1252:1256	fungi	1252:1256	All pathogens, in particular S. aureus and fungi, significantly increased SCC in both parity groups.
30101726	9	78	from	pathogens	1213:1221	arg1	aureus					1241:1246	S. aureus	1238:1246	S. aureus	1238:1246	All pathogens, in particular S. aureus and fungi, significantly increased SCC in both parity groups.
30101726	14	79	theme	CM	1722:1723	arg1	costs					1713:1717	precise costs	1705:1717	precise costs of CM	1705:1723	These findings provide estimates that could be used to calculate precise costs of CM, and also provide better indicators of pathogen-specific mastitis.
30101726	1	80	theme	somatic	289:295	arg1	SCC					309:311	SCC	309:311	SCC	309:311	The aim of this study was to estimate the associations of the first occurrence of pathogen-specific clinical mastitis (CM) with milk yield and milk composition (somatic cell count (SCC), lactose, fat, protein content in milk and milk urea nitrogen (MUN)).
30101726	1	80	theme	somatic	289:295	arg1	count					302:306	somatic cell count	289:306	somatic cell count (SCC)	289:312	The aim of this study was to estimate the associations of the first occurrence of pathogen-specific clinical mastitis (CM) with milk yield and milk composition (somatic cell count (SCC), lactose, fat, protein content in milk and milk urea nitrogen (MUN)).
30101726	6	81	theme	later	952:956	arg1	lactations					958:967	second and later lactations	941:967	second and later lactations	941:967	Primipara (first lactation) and multipara (second and later lactations) were analysed separately.
30101726	6	81	theme	later	952:956	arg1	multipara					930:938	multipara	930:938	multipara (second and later lactations)	930:968	Primipara (first lactation) and multipara (second and later lactations) were analysed separately.
30101726	3	82	theme	coagulase-negative	538:555	arg1	staphylococci					557:569	coagulase-negative staphylococci	538:569	coagulase-negative staphylococci (CNS)	538:575	Five pathogen groups were studied: Streptococcus spp.; Staphylococcus aureus (S. aureus); coagulase-negative staphylococci (CNS); coliforms; and fungi.
30101726	3	82	theme	coagulase-negative	538:555	arg1	CNS					572:574	CNS	572:574	CNS	572:574	Five pathogen groups were studied: Streptococcus spp.; Staphylococcus aureus (S. aureus); coagulase-negative staphylococci (CNS); coliforms; and fungi.
30101726	4	83	theme	June	660:663	arg1	2011					665:668	June 2011	660:668	June 2011	660:668	Test-day milk data and clinical records were collected from June 2011 until February 2014.
30101726	1	84	theme	first	190:194	arg1	occurrence					196:205	the first occurrence	186:205	the first occurrence of pathogen-specific clinical mastitis (CM)	186:249	The aim of this study was to estimate the associations of the first occurrence of pathogen-specific clinical mastitis (CM) with milk yield and milk composition (somatic cell count (SCC), lactose, fat, protein content in milk and milk urea nitrogen (MUN)).
30101726	6	85	theme	second	941:946	arg1	lactations					958:967	second and later lactations	941:967	second and later lactations	941:967	Primipara (first lactation) and multipara (second and later lactations) were analysed separately.
30101726	6	85	theme	second	941:946	arg1	multipara					930:938	multipara	930:938	multipara (second and later lactations)	930:968	Primipara (first lactation) and multipara (second and later lactations) were analysed separately.
30101726	1	86	theme	clinical	228:235	arg1	CM					247:248	CM	247:248	CM	247:248	The aim of this study was to estimate the associations of the first occurrence of pathogen-specific clinical mastitis (CM) with milk yield and milk composition (somatic cell count (SCC), lactose, fat, protein content in milk and milk urea nitrogen (MUN)).
30101726	1	86	theme	clinical	228:235	arg1	mastitis					237:244	pathogen-specific clinical mastitis	210:244	pathogen-specific clinical mastitis (CM)	210:249	The aim of this study was to estimate the associations of the first occurrence of pathogen-specific clinical mastitis (CM) with milk yield and milk composition (somatic cell count (SCC), lactose, fat, protein content in milk and milk urea nitrogen (MUN)).
30502425	0	0	theme	carrier	72:78	arg1	delivery					80:87	smart nanocaged carrier delivery	56:87	smart nanocaged carrier delivery of multifunctional PEG-chitosan	56:119	Design, optimization and in vitro-in vivo evaluation of smart nanocaged carrier delivery of multifunctional PEG-chitosan stabilized silybin nanocrystals.
30502425	9	1	theme	nanocaged	1418:1426	arg1	nanocrystals					1428:1439	the nanocaged nanocrystals	1414:1439	the nanocaged nanocrystals	1414:1439	Compared with the silybin solution, the nanocaged nanocrystals and the PEG-CS nanocrystals drug delivery system also exhibited 5.54 and 2.58 fold increasing in the AUC, respectively.
30502425	10	2	theme	nanocrystals	1581:1592	arg1	approach					1735:1742	an efficient and feasible approach	1709:1742	an efficient and feasible approach for water insoluble drugs delivery	1709:1777	Thus, the nanocaged nanocrystals technology with excellent stability, improved dissolution velocity and relative bioavailability was recommended as an efficient and feasible approach for water insoluble drugs delivery.
30502425	10	2	theme	nanocrystals	1581:1592	arg1	technology					1594:1603	the nanocaged nanocrystals technology	1567:1603	the nanocaged nanocrystals technology with excellent stability, improved dissolution velocity and relative bioavailability	1567:1688	Thus, the nanocaged nanocrystals technology with excellent stability, improved dissolution velocity and relative bioavailability was recommended as an efficient and feasible approach for water insoluble drugs delivery.
30502425	9	3	theme	drug	1469:1472	arg1	system					1483:1488	drug delivery system	1469:1488	the PEG-CS nanocrystals drug delivery system	1445:1488	Compared with the silybin solution, the nanocaged nanocrystals and the PEG-CS nanocrystals drug delivery system also exhibited 5.54 and 2.58 fold increasing in the AUC, respectively.
30502425	5	4	theme	scanning	978:985	arg1	microscopy					966:975	The transmission electron microscopy	940:975	The transmission electron microscopy	940:975	The transmission electron microscopy, scanning electron microscopy and atomic force microscope revealed small and uniformed morphology.
30502425	5	4	theme	scanning	978:985	arg1	microscopy					996:1005	scanning electron microscopy	978:1005	scanning electron microscopy	978:1005	The transmission electron microscopy, scanning electron microscopy and atomic force microscope revealed small and uniformed morphology.
30502425	8	5	theme	nanocaged	1318:1326	arg1	nanocrystals					1328:1339	the nanocaged nanocrystals	1314:1339	the nanocaged nanocrystals	1314:1339	During the long term stability study, the nanocaged nanocrystals were presented remarkable stability.
30502425	1	6	theme	mPEG-chitosan	272:284	arg1	nanocrystals					305:316	multifunctional mPEG-chitosan stabilized silybin nanocrystals	256:316	multifunctional mPEG-chitosan stabilized silybin nanocrystals	256:316	This study explored the feasibility of using smart nanocaged carrier technology for the delivering of multifunctional mPEG-chitosan stabilized silybin nanocrystals and to enhancing the long-term stability, dissolution velocity and bioavailability of the water insoluble drugs.
30502425	0	7	theme	nanocaged	62:70	arg1	delivery					80:87	smart nanocaged carrier delivery	56:87	smart nanocaged carrier delivery of multifunctional PEG-chitosan	56:119	Design, optimization and in vitro-in vivo evaluation of smart nanocaged carrier delivery of multifunctional PEG-chitosan stabilized silybin nanocrystals.
30502425	3	8	theme	nanocrystals	707:718	arg1	formulation					720:730	the silybin nanocrystals formulation	695:730	the silybin nanocrystals formulation	695:730	The methoxypolythylene glycol-grafted chitosan (PEG-CS), as multifunctional stabilizer, were applied for the silybin nanocrystals formulation.
30502425	8	9	dep	term	1292:1295	arg1	long					1287:1290	long	1287:1290	long	1287:1290	During the long term stability study, the nanocaged nanocrystals were presented remarkable stability.
30502425	5	10	theme	electron	987:994	arg1	microscopy					966:975	The transmission electron microscopy	940:975	The transmission electron microscopy	940:975	The transmission electron microscopy, scanning electron microscopy and atomic force microscope revealed small and uniformed morphology.
30502425	5	10	theme	electron	987:994	arg1	microscopy					996:1005	scanning electron microscopy	978:1005	scanning electron microscopy	978:1005	The transmission electron microscopy, scanning electron microscopy and atomic force microscope revealed small and uniformed morphology.
30502425	1	11	theme	stabilized	286:295	arg1	nanocrystals					305:316	multifunctional mPEG-chitosan stabilized silybin nanocrystals	256:316	multifunctional mPEG-chitosan stabilized silybin nanocrystals	256:316	This study explored the feasibility of using smart nanocaged carrier technology for the delivering of multifunctional mPEG-chitosan stabilized silybin nanocrystals and to enhancing the long-term stability, dissolution velocity and bioavailability of the water insoluble drugs.
30502425	4	12	theme	central	891:897	arg1	model					933:937	central composite design-response surface model	891:937	central composite design-response surface model	891:937	Two silybin nanocrystals formulations (PEG-CS nanocrystals and nanocaged nanocrystals) were prepared using anti-solvent precipitation method and optimized by central composite design-response surface model.
30502425	5	13	theme	uniformed	1054:1062	arg1	morphology					1064:1073	small and uniformed morphology	1044:1073	small and uniformed morphology	1044:1073	The transmission electron microscopy, scanning electron microscopy and atomic force microscope revealed small and uniformed morphology.
30502425	1	14	theme	silybin	297:303	arg1	nanocrystals					305:316	multifunctional mPEG-chitosan stabilized silybin nanocrystals	256:316	multifunctional mPEG-chitosan stabilized silybin nanocrystals	256:316	This study explored the feasibility of using smart nanocaged carrier technology for the delivering of multifunctional mPEG-chitosan stabilized silybin nanocrystals and to enhancing the long-term stability, dissolution velocity and bioavailability of the water insoluble drugs.
30502425	0	15	theme	delivery	80:87	arg1	optimization					8:19	optimization	8:19	optimization	8:19	Design, optimization and in vitro-in vivo evaluation of smart nanocaged carrier delivery of multifunctional PEG-chitosan stabilized silybin nanocrystals.
30502425	0	15	theme	delivery	80:87	arg1	Design					0:5	Design	0:5	Design	0:5	Design, optimization and in vitro-in vivo evaluation of smart nanocaged carrier delivery of multifunctional PEG-chitosan stabilized silybin nanocrystals.
30502425	0	15	theme	delivery	80:87	arg1	evaluation					42:51	in vitro-in vivo evaluation	25:51	in vitro-in vivo evaluation of smart nanocaged carrier delivery of multifunctional PEG-chitosan	25:119	Design, optimization and in vitro-in vivo evaluation of smart nanocaged carrier delivery of multifunctional PEG-chitosan stabilized silybin nanocrystals.
30502425	6	16	theme	crystalline	1080:1090	arg1	state					1092:1096	The crystalline state	1076:1096	The crystalline state of the nanocrystals	1076:1116	The crystalline state of the nanocrystals was confirmed by X-ray powder diffraction and differential scanning calorimetry.
30502425	0	17	theme	PEG-chitosan	108:119	arg1	delivery					80:87	smart nanocaged carrier delivery	56:87	smart nanocaged carrier delivery of multifunctional PEG-chitosan	56:119	Design, optimization and in vitro-in vivo evaluation of smart nanocaged carrier delivery of multifunctional PEG-chitosan stabilized silybin nanocrystals.
30502425	1	18	theme	water	408:412	arg1	drugs					424:428	the water insoluble drugs	404:428	the water insoluble drugs	404:428	This study explored the feasibility of using smart nanocaged carrier technology for the delivering of multifunctional mPEG-chitosan stabilized silybin nanocrystals and to enhancing the long-term stability, dissolution velocity and bioavailability of the water insoluble drugs.
30502425	4	19	theme	design-response	909:923	arg1	model					933:937	central composite design-response surface model	891:937	central composite design-response surface model	891:937	Two silybin nanocrystals formulations (PEG-CS nanocrystals and nanocaged nanocrystals) were prepared using anti-solvent precipitation method and optimized by central composite design-response surface model.
30502425	10	20	theme	nanocaged	1571:1579	arg1	approach					1735:1742	an efficient and feasible approach	1709:1742	an efficient and feasible approach for water insoluble drugs delivery	1709:1777	Thus, the nanocaged nanocrystals technology with excellent stability, improved dissolution velocity and relative bioavailability was recommended as an efficient and feasible approach for water insoluble drugs delivery.
30502425	10	20	theme	nanocaged	1571:1579	arg1	technology					1594:1603	the nanocaged nanocrystals technology	1567:1603	the nanocaged nanocrystals technology with excellent stability, improved dissolution velocity and relative bioavailability	1567:1688	Thus, the nanocaged nanocrystals technology with excellent stability, improved dissolution velocity and relative bioavailability was recommended as an efficient and feasible approach for water insoluble drugs delivery.
30502425	6	21	theme	scanning	1177:1184	arg1	calorimetry					1186:1196	differential scanning calorimetry	1164:1196	differential scanning calorimetry	1164:1196	The crystalline state of the nanocrystals was confirmed by X-ray powder diffraction and differential scanning calorimetry.
30502425	0	22	theme	multifunctional	92:106	arg1	PEG-chitosan					108:119	multifunctional PEG-chitosan	92:119	multifunctional PEG-chitosan	92:119	Design, optimization and in vitro-in vivo evaluation of smart nanocaged carrier delivery of multifunctional PEG-chitosan stabilized silybin nanocrystals.
30502425	1	23	theme	insoluble	414:422	arg1	drugs					424:428	the water insoluble drugs	404:428	the water insoluble drugs	404:428	This study explored the feasibility of using smart nanocaged carrier technology for the delivering of multifunctional mPEG-chitosan stabilized silybin nanocrystals and to enhancing the long-term stability, dissolution velocity and bioavailability of the water insoluble drugs.
30502425	2	24	mod	modified	527:534	arg3	group					561:565	azide group	555:565	azide group	555:565	The methoxypoly(ethylene glycol)-b-poly(ε-caprolactone) (mPEG-b-PCL), as nanocaged carrier, was modified with propargyl and azide group and confirmed by FTIR.
30502425	2	24	mod	modified	527:534	arg1	ε-caprolactone					471:484	ε-caprolactone	471:484	ε-caprolactone	471:484	The methoxypoly(ethylene glycol)-b-poly(ε-caprolactone) (mPEG-b-PCL), as nanocaged carrier, was modified with propargyl and azide group and confirmed by FTIR.
30502425	2	24	mod	modified	527:534	arg3	propargyl					541:549	propargyl	541:549	propargyl	541:549	The methoxypoly(ethylene glycol)-b-poly(ε-caprolactone) (mPEG-b-PCL), as nanocaged carrier, was modified with propargyl and azide group and confirmed by FTIR.
30502425	2	24	mod	modified	527:534	arg1	glycol					456:461	ethylene glycol	447:461	ethylene glycol	447:461	The methoxypoly(ethylene glycol)-b-poly(ε-caprolactone) (mPEG-b-PCL), as nanocaged carrier, was modified with propargyl and azide group and confirmed by FTIR.
30502425	2	24	mod	modified	527:534	arg1	mPEG-b-PCL					488:497	mPEG-b-PCL	488:497	mPEG-b-PCL	488:497	The methoxypoly(ethylene glycol)-b-poly(ε-caprolactone) (mPEG-b-PCL), as nanocaged carrier, was modified with propargyl and azide group and confirmed by FTIR.
30502425	2	24	mod	modified	527:534	arg1	-b-poly					463:469	The methoxypoly(ethylene glycol)-b-poly	431:469	The methoxypoly(ethylene glycol)-b-poly(ε-caprolactone) (mPEG-b-PCL)	431:498	The methoxypoly(ethylene glycol)-b-poly(ε-caprolactone) (mPEG-b-PCL), as nanocaged carrier, was modified with propargyl and azide group and confirmed by FTIR.
30502425	2	25	theme	methoxypoly	435:445	arg1	glycol					456:461	ethylene glycol	447:461	ethylene glycol	447:461	The methoxypoly(ethylene glycol)-b-poly(ε-caprolactone) (mPEG-b-PCL), as nanocaged carrier, was modified with propargyl and azide group and confirmed by FTIR.
30502425	2	25	theme	methoxypoly	435:445	arg1	ε-caprolactone					471:484	ε-caprolactone	471:484	ε-caprolactone	471:484	The methoxypoly(ethylene glycol)-b-poly(ε-caprolactone) (mPEG-b-PCL), as nanocaged carrier, was modified with propargyl and azide group and confirmed by FTIR.
30502425	2	25	theme	methoxypoly	435:445	arg1	mPEG-b-PCL					488:497	mPEG-b-PCL	488:497	mPEG-b-PCL	488:497	The methoxypoly(ethylene glycol)-b-poly(ε-caprolactone) (mPEG-b-PCL), as nanocaged carrier, was modified with propargyl and azide group and confirmed by FTIR.
30502425	2	25	theme	methoxypoly	435:445	arg1	-b-poly					463:469	The methoxypoly(ethylene glycol)-b-poly	431:469	The methoxypoly(ethylene glycol)-b-poly(ε-caprolactone) (mPEG-b-PCL)	431:498	The methoxypoly(ethylene glycol)-b-poly(ε-caprolactone) (mPEG-b-PCL), as nanocaged carrier, was modified with propargyl and azide group and confirmed by FTIR.
30502425	1	26	theme	drugs	424:428	arg1	bioavailability					385:399	bioavailability	385:399	bioavailability	385:399	This study explored the feasibility of using smart nanocaged carrier technology for the delivering of multifunctional mPEG-chitosan stabilized silybin nanocrystals and to enhancing the long-term stability, dissolution velocity and bioavailability of the water insoluble drugs.
30502425	1	26	theme	drugs	424:428	arg1	stability					349:357	long-term stability	339:357	long-term stability	339:357	This study explored the feasibility of using smart nanocaged carrier technology for the delivering of multifunctional mPEG-chitosan stabilized silybin nanocrystals and to enhancing the long-term stability, dissolution velocity and bioavailability of the water insoluble drugs.
30502425	1	26	theme	drugs	424:428	arg1	velocity					372:379	dissolution velocity	360:379	dissolution velocity	360:379	This study explored the feasibility of using smart nanocaged carrier technology for the delivering of multifunctional mPEG-chitosan stabilized silybin nanocrystals and to enhancing the long-term stability, dissolution velocity and bioavailability of the water insoluble drugs.
30502425	10	27	theme	efficient	1712:1720	arg1	approach					1735:1742	an efficient and feasible approach	1709:1742	an efficient and feasible approach for water insoluble drugs delivery	1709:1777	Thus, the nanocaged nanocrystals technology with excellent stability, improved dissolution velocity and relative bioavailability was recommended as an efficient and feasible approach for water insoluble drugs delivery.
30502425	10	27	theme	efficient	1712:1720	arg1	technology					1594:1603	the nanocaged nanocrystals technology	1567:1603	the nanocaged nanocrystals technology with excellent stability, improved dissolution velocity and relative bioavailability	1567:1688	Thus, the nanocaged nanocrystals technology with excellent stability, improved dissolution velocity and relative bioavailability was recommended as an efficient and feasible approach for water insoluble drugs delivery.
30502425	9	28	theme	PEG-CS	1449:1454	arg1	nanocrystals					1456:1467	the PEG-CS nanocrystals drug delivery system	1445:1488	the PEG-CS nanocrystals drug delivery system	1445:1488	Compared with the silybin solution, the nanocaged nanocrystals and the PEG-CS nanocrystals drug delivery system also exhibited 5.54 and 2.58 fold increasing in the AUC, respectively.
30502425	5	29	theme	transmission	944:955	arg1	microscopy					966:975	The transmission electron microscopy	940:975	The transmission electron microscopy	940:975	The transmission electron microscopy, scanning electron microscopy and atomic force microscope revealed small and uniformed morphology.
30502425	5	29	theme	transmission	944:955	arg1	microscope					1024:1033	atomic force microscope	1011:1033	atomic force microscope	1011:1033	The transmission electron microscopy, scanning electron microscopy and atomic force microscope revealed small and uniformed morphology.
30502425	5	29	theme	transmission	944:955	arg1	microscopy					996:1005	scanning electron microscopy	978:1005	scanning electron microscopy	978:1005	The transmission electron microscopy, scanning electron microscopy and atomic force microscope revealed small and uniformed morphology.
30502425	10	30	theme	drugs	1764:1768	arg1	delivery					1770:1777	water insoluble drugs delivery	1748:1777	water insoluble drugs delivery	1748:1777	Thus, the nanocaged nanocrystals technology with excellent stability, improved dissolution velocity and relative bioavailability was recommended as an efficient and feasible approach for water insoluble drugs delivery.
30502425	9	31	theme	delivery	1474:1481	arg1	system					1483:1488	drug delivery system	1469:1488	the PEG-CS nanocrystals drug delivery system	1445:1488	Compared with the silybin solution, the nanocaged nanocrystals and the PEG-CS nanocrystals drug delivery system also exhibited 5.54 and 2.58 fold increasing in the AUC, respectively.
30502425	4	32	theme	surface	925:931	arg1	model					933:937	central composite design-response surface model	891:937	central composite design-response surface model	891:937	Two silybin nanocrystals formulations (PEG-CS nanocrystals and nanocaged nanocrystals) were prepared using anti-solvent precipitation method and optimized by central composite design-response surface model.
30502425	4	33	theme	PEG-CS	772:777	arg1	nanocrystals					779:790	PEG-CS nanocrystals	772:790	PEG-CS nanocrystals	772:790	Two silybin nanocrystals formulations (PEG-CS nanocrystals and nanocaged nanocrystals) were prepared using anti-solvent precipitation method and optimized by central composite design-response surface model.
30502425	1	34	theme	smart	199:203	arg1	technology					223:232	smart nanocaged carrier technology	199:232	smart nanocaged carrier technology for the delivering of multifunctional mPEG-chitosan stabilized silybin nanocrystals	199:316	This study explored the feasibility of using smart nanocaged carrier technology for the delivering of multifunctional mPEG-chitosan stabilized silybin nanocrystals and to enhancing the long-term stability, dissolution velocity and bioavailability of the water insoluble drugs.
30502425	7	35	dep	Fourier	1235:1241	arg1	transform					1243:1251	transform	1243:1251	transform infrared spectroscopy	1243:1273	The drug structure was confirmed by Fourier transform infrared spectroscopy.
30502425	8	36	theme	term	1292:1295	arg1	study					1307:1311	the long term stability study	1283:1311	the long term stability study	1283:1311	During the long term stability study, the nanocaged nanocrystals were presented remarkable stability.
30502425	0	37	theme	silybin	132:138	arg1	nanocrystals					140:151	silybin nanocrystals	132:151	silybin nanocrystals	132:151	Design, optimization and in vitro-in vivo evaluation of smart nanocaged carrier delivery of multifunctional PEG-chitosan stabilized silybin nanocrystals.
30502425	3	38	theme	glycol-grafted	613:626	arg1	PEG-CS					638:643	The methoxypolythylene glycol-grafted chitosan (PEG-CS)	590:644	The methoxypolythylene glycol-grafted chitosan (PEG-CS)	590:644	The methoxypolythylene glycol-grafted chitosan (PEG-CS), as multifunctional stabilizer, were applied for the silybin nanocrystals formulation.
30502425	4	39	theme	nanocaged	796:804	arg1	nanocrystals					806:817	nanocaged nanocrystals	796:817	nanocaged nanocrystals	796:817	Two silybin nanocrystals formulations (PEG-CS nanocrystals and nanocaged nanocrystals) were prepared using anti-solvent precipitation method and optimized by central composite design-response surface model.
30502425	10	40	with	technology	1594:1603	arg1	velocity					1652:1659	improved dissolution velocity	1631:1659	improved dissolution velocity	1631:1659	Thus, the nanocaged nanocrystals technology with excellent stability, improved dissolution velocity and relative bioavailability was recommended as an efficient and feasible approach for water insoluble drugs delivery.
30502425	10	40	with	technology	1594:1603	arg1	bioavailability					1674:1688	relative bioavailability	1665:1688	relative bioavailability	1665:1688	Thus, the nanocaged nanocrystals technology with excellent stability, improved dissolution velocity and relative bioavailability was recommended as an efficient and feasible approach for water insoluble drugs delivery.
30502425	10	40	with	technology	1594:1603	arg1	stability					1620:1628	excellent stability	1610:1628	excellent stability	1610:1628	Thus, the nanocaged nanocrystals technology with excellent stability, improved dissolution velocity and relative bioavailability was recommended as an efficient and feasible approach for water insoluble drugs delivery.
30502425	1	41	theme	nanocaged	205:213	arg1	technology					223:232	smart nanocaged carrier technology	199:232	smart nanocaged carrier technology for the delivering of multifunctional mPEG-chitosan stabilized silybin nanocrystals	199:316	This study explored the feasibility of using smart nanocaged carrier technology for the delivering of multifunctional mPEG-chitosan stabilized silybin nanocrystals and to enhancing the long-term stability, dissolution velocity and bioavailability of the water insoluble drugs.
30502425	4	42	dep	formulations	758:769	arg1	nanocrystals					779:790	PEG-CS nanocrystals	772:790	PEG-CS nanocrystals	772:790	Two silybin nanocrystals formulations (PEG-CS nanocrystals and nanocaged nanocrystals) were prepared using anti-solvent precipitation method and optimized by central composite design-response surface model.
30502425	4	42	dep	formulations	758:769	arg1	nanocrystals					806:817	nanocaged nanocrystals	796:817	nanocaged nanocrystals	796:817	Two silybin nanocrystals formulations (PEG-CS nanocrystals and nanocaged nanocrystals) were prepared using anti-solvent precipitation method and optimized by central composite design-response surface model.
30502425	10	43	theme	dissolution	1640:1650	arg1	velocity					1652:1659	improved dissolution velocity	1631:1659	improved dissolution velocity	1631:1659	Thus, the nanocaged nanocrystals technology with excellent stability, improved dissolution velocity and relative bioavailability was recommended as an efficient and feasible approach for water insoluble drugs delivery.
30502425	1	44	theme	carrier	215:221	arg1	technology					223:232	smart nanocaged carrier technology	199:232	smart nanocaged carrier technology for the delivering of multifunctional mPEG-chitosan stabilized silybin nanocrystals	199:316	This study explored the feasibility of using smart nanocaged carrier technology for the delivering of multifunctional mPEG-chitosan stabilized silybin nanocrystals and to enhancing the long-term stability, dissolution velocity and bioavailability of the water insoluble drugs.
30502425	10	45	theme	feasible	1726:1733	arg1	approach					1735:1742	an efficient and feasible approach	1709:1742	an efficient and feasible approach for water insoluble drugs delivery	1709:1777	Thus, the nanocaged nanocrystals technology with excellent stability, improved dissolution velocity and relative bioavailability was recommended as an efficient and feasible approach for water insoluble drugs delivery.
30502425	10	45	theme	feasible	1726:1733	arg1	technology					1594:1603	the nanocaged nanocrystals technology	1567:1603	the nanocaged nanocrystals technology with excellent stability, improved dissolution velocity and relative bioavailability	1567:1688	Thus, the nanocaged nanocrystals technology with excellent stability, improved dissolution velocity and relative bioavailability was recommended as an efficient and feasible approach for water insoluble drugs delivery.
30502425	10	46	theme	improved	1631:1638	arg1	velocity					1652:1659	improved dissolution velocity	1631:1659	improved dissolution velocity	1631:1659	Thus, the nanocaged nanocrystals technology with excellent stability, improved dissolution velocity and relative bioavailability was recommended as an efficient and feasible approach for water insoluble drugs delivery.
30502425	10	47	theme	water	1748:1752	arg1	delivery					1770:1777	water insoluble drugs delivery	1748:1777	water insoluble drugs delivery	1748:1777	Thus, the nanocaged nanocrystals technology with excellent stability, improved dissolution velocity and relative bioavailability was recommended as an efficient and feasible approach for water insoluble drugs delivery.
30502425	3	48	theme	chitosan	628:635	arg1	PEG-CS					638:643	The methoxypolythylene glycol-grafted chitosan (PEG-CS)	590:644	The methoxypolythylene glycol-grafted chitosan (PEG-CS)	590:644	The methoxypolythylene glycol-grafted chitosan (PEG-CS), as multifunctional stabilizer, were applied for the silybin nanocrystals formulation.
30502425	2	49	theme	nanocaged	504:512	arg1	carrier					514:520	nanocaged carrier	504:520	nanocaged carrier	504:520	The methoxypoly(ethylene glycol)-b-poly(ε-caprolactone) (mPEG-b-PCL), as nanocaged carrier, was modified with propargyl and azide group and confirmed by FTIR.
30502425	6	50	theme	powder	1141:1146	arg1	diffraction					1148:1158	X-ray powder diffraction	1135:1158	X-ray powder diffraction	1135:1158	The crystalline state of the nanocrystals was confirmed by X-ray powder diffraction and differential scanning calorimetry.
30502425	0	51	theme	in	25:26	arg1	evaluation					42:51	in vitro-in vivo evaluation	25:51	in vitro-in vivo evaluation of smart nanocaged carrier delivery of multifunctional PEG-chitosan	25:119	Design, optimization and in vitro-in vivo evaluation of smart nanocaged carrier delivery of multifunctional PEG-chitosan stabilized silybin nanocrystals.
30502425	1	52	theme	long-term	339:347	arg1	stability					349:357	long-term stability	339:357	long-term stability	339:357	This study explored the feasibility of using smart nanocaged carrier technology for the delivering of multifunctional mPEG-chitosan stabilized silybin nanocrystals and to enhancing the long-term stability, dissolution velocity and bioavailability of the water insoluble drugs.
30502425	2	53	theme	azide	555:559	arg1	group					561:565	azide group	555:565	azide group	555:565	The methoxypoly(ethylene glycol)-b-poly(ε-caprolactone) (mPEG-b-PCL), as nanocaged carrier, was modified with propargyl and azide group and confirmed by FTIR.
30502425	3	54	theme	multifunctional	650:664	arg1	stabilizer					666:675	multifunctional stabilizer	650:675	multifunctional stabilizer	650:675	The methoxypolythylene glycol-grafted chitosan (PEG-CS), as multifunctional stabilizer, were applied for the silybin nanocrystals formulation.
30502425	6	55	theme	nanocrystals	1105:1116	arg1	state					1092:1096	The crystalline state	1076:1096	The crystalline state of the nanocrystals	1076:1116	The crystalline state of the nanocrystals was confirmed by X-ray powder diffraction and differential scanning calorimetry.
30502425	2	56	theme	ethylene	447:454	arg1	glycol					456:461	ethylene glycol	447:461	ethylene glycol	447:461	The methoxypoly(ethylene glycol)-b-poly(ε-caprolactone) (mPEG-b-PCL), as nanocaged carrier, was modified with propargyl and azide group and confirmed by FTIR.
30502425	2	56	theme	ethylene	447:454	arg1	-b-poly					463:469	The methoxypoly(ethylene glycol)-b-poly	431:469	The methoxypoly(ethylene glycol)-b-poly(ε-caprolactone) (mPEG-b-PCL)	431:498	The methoxypoly(ethylene glycol)-b-poly(ε-caprolactone) (mPEG-b-PCL), as nanocaged carrier, was modified with propargyl and azide group and confirmed by FTIR.
30502425	8	57	theme	remarkable	1356:1365	arg1	stability					1367:1375	remarkable stability	1356:1375	remarkable stability	1356:1375	During the long term stability study, the nanocaged nanocrystals were presented remarkable stability.
30502425	9	58	dep	nanocrystals	1456:1467	arg1	system					1483:1488	drug delivery system	1469:1488	the PEG-CS nanocrystals drug delivery system	1445:1488	Compared with the silybin solution, the nanocaged nanocrystals and the PEG-CS nanocrystals drug delivery system also exhibited 5.54 and 2.58 fold increasing in the AUC, respectively.
30502425	3	59	theme	methoxypolythylene	594:611	arg1	PEG-CS					638:643	The methoxypolythylene glycol-grafted chitosan (PEG-CS)	590:644	The methoxypolythylene glycol-grafted chitosan (PEG-CS)	590:644	The methoxypolythylene glycol-grafted chitosan (PEG-CS), as multifunctional stabilizer, were applied for the silybin nanocrystals formulation.
30502425	7	60	dep	transform	1243:1251	arg1	infrared					1253:1260	infrared	1253:1260	transform infrared spectroscopy	1243:1273	The drug structure was confirmed by Fourier transform infrared spectroscopy.
30502425	4	61	theme	nanocrystals	745:756	arg1	formulations					758:769	Two silybin nanocrystals formulations	733:769	Two silybin nanocrystals formulations (PEG-CS nanocrystals and nanocaged nanocrystals)	733:818	Two silybin nanocrystals formulations (PEG-CS nanocrystals and nanocaged nanocrystals) were prepared using anti-solvent precipitation method and optimized by central composite design-response surface model.
30502425	5	62	theme	small	1044:1048	arg1	morphology					1064:1073	small and uniformed morphology	1044:1073	small and uniformed morphology	1044:1073	The transmission electron microscopy, scanning electron microscopy and atomic force microscope revealed small and uniformed morphology.
30502425	4	63	theme	composite	899:907	arg1	model					933:937	central composite design-response surface model	891:937	central composite design-response surface model	891:937	Two silybin nanocrystals formulations (PEG-CS nanocrystals and nanocaged nanocrystals) were prepared using anti-solvent precipitation method and optimized by central composite design-response surface model.
30502425	0	64	dep	in	25:26	arg1	vivo					37:40	vivo	37:40	vivo	37:40	Design, optimization and in vitro-in vivo evaluation of smart nanocaged carrier delivery of multifunctional PEG-chitosan stabilized silybin nanocrystals.
30502425	4	65	theme	silybin	737:743	arg1	formulations					758:769	Two silybin nanocrystals formulations	733:769	Two silybin nanocrystals formulations (PEG-CS nanocrystals and nanocaged nanocrystals)	733:818	Two silybin nanocrystals formulations (PEG-CS nanocrystals and nanocaged nanocrystals) were prepared using anti-solvent precipitation method and optimized by central composite design-response surface model.
30502425	10	66	theme	excellent	1610:1618	arg1	stability					1620:1628	excellent stability	1610:1628	excellent stability	1610:1628	Thus, the nanocaged nanocrystals technology with excellent stability, improved dissolution velocity and relative bioavailability was recommended as an efficient and feasible approach for water insoluble drugs delivery.
30502425	1	67	theme	dissolution	360:370	arg1	velocity					372:379	dissolution velocity	360:379	dissolution velocity	360:379	This study explored the feasibility of using smart nanocaged carrier technology for the delivering of multifunctional mPEG-chitosan stabilized silybin nanocrystals and to enhancing the long-term stability, dissolution velocity and bioavailability of the water insoluble drugs.
30502425	10	68	theme	relative	1665:1672	arg1	bioavailability					1674:1688	relative bioavailability	1665:1688	relative bioavailability	1665:1688	Thus, the nanocaged nanocrystals technology with excellent stability, improved dissolution velocity and relative bioavailability was recommended as an efficient and feasible approach for water insoluble drugs delivery.
30502425	3	69	theme	silybin	699:705	arg1	formulation					720:730	the silybin nanocrystals formulation	695:730	the silybin nanocrystals formulation	695:730	The methoxypolythylene glycol-grafted chitosan (PEG-CS), as multifunctional stabilizer, were applied for the silybin nanocrystals formulation.
30502425	4	70	theme	anti-solvent	840:851	arg1	method					867:872	anti-solvent precipitation method	840:872	anti-solvent precipitation method	840:872	Two silybin nanocrystals formulations (PEG-CS nanocrystals and nanocaged nanocrystals) were prepared using anti-solvent precipitation method and optimized by central composite design-response surface model.
30502425	5	71	theme	atomic	1011:1016	arg1	microscope					1024:1033	atomic force microscope	1011:1033	atomic force microscope	1011:1033	The transmission electron microscopy, scanning electron microscopy and atomic force microscope revealed small and uniformed morphology.
30502425	5	71	theme	atomic	1011:1016	arg1	microscopy					966:975	The transmission electron microscopy	940:975	The transmission electron microscopy	940:975	The transmission electron microscopy, scanning electron microscopy and atomic force microscope revealed small and uniformed morphology.
30502425	5	72	theme	electron	957:964	arg1	microscopy					966:975	The transmission electron microscopy	940:975	The transmission electron microscopy	940:975	The transmission electron microscopy, scanning electron microscopy and atomic force microscope revealed small and uniformed morphology.
30502425	5	72	theme	electron	957:964	arg1	microscope					1024:1033	atomic force microscope	1011:1033	atomic force microscope	1011:1033	The transmission electron microscopy, scanning electron microscopy and atomic force microscope revealed small and uniformed morphology.
30502425	5	72	theme	electron	957:964	arg1	microscopy					996:1005	scanning electron microscopy	978:1005	scanning electron microscopy	978:1005	The transmission electron microscopy, scanning electron microscopy and atomic force microscope revealed small and uniformed morphology.
30502425	6	73	theme	differential	1164:1175	arg1	calorimetry					1186:1196	differential scanning calorimetry	1164:1196	differential scanning calorimetry	1164:1196	The crystalline state of the nanocrystals was confirmed by X-ray powder diffraction and differential scanning calorimetry.
30502425	5	74	theme	force	1018:1022	arg1	microscope					1024:1033	atomic force microscope	1011:1033	atomic force microscope	1011:1033	The transmission electron microscopy, scanning electron microscopy and atomic force microscope revealed small and uniformed morphology.
30502425	5	74	theme	force	1018:1022	arg1	microscopy					966:975	The transmission electron microscopy	940:975	The transmission electron microscopy	940:975	The transmission electron microscopy, scanning electron microscopy and atomic force microscope revealed small and uniformed morphology.
30502425	7	75	theme	drug	1203:1206	arg1	structure					1208:1216	The drug structure	1199:1216	The drug structure	1199:1216	The drug structure was confirmed by Fourier transform infrared spectroscopy.
30502425	0	76	theme	smart	56:60	arg1	delivery					80:87	smart nanocaged carrier delivery	56:87	smart nanocaged carrier delivery of multifunctional PEG-chitosan	56:119	Design, optimization and in vitro-in vivo evaluation of smart nanocaged carrier delivery of multifunctional PEG-chitosan stabilized silybin nanocrystals.
30502425	6	77	theme	X-ray	1135:1139	arg1	diffraction					1148:1158	X-ray powder diffraction	1135:1158	X-ray powder diffraction	1135:1158	The crystalline state of the nanocrystals was confirmed by X-ray powder diffraction and differential scanning calorimetry.
30502425	10	78	theme	insoluble	1754:1762	arg1	delivery					1770:1777	water insoluble drugs delivery	1748:1777	water insoluble drugs delivery	1748:1777	Thus, the nanocaged nanocrystals technology with excellent stability, improved dissolution velocity and relative bioavailability was recommended as an efficient and feasible approach for water insoluble drugs delivery.
30502425	8	79	theme	stability	1297:1305	arg1	study					1307:1311	the long term stability study	1283:1311	the long term stability study	1283:1311	During the long term stability study, the nanocaged nanocrystals were presented remarkable stability.
30502425	9	80	theme	silybin	1396:1402	arg1	solution					1404:1411	the silybin solution	1392:1411	the silybin solution	1392:1411	Compared with the silybin solution, the nanocaged nanocrystals and the PEG-CS nanocrystals drug delivery system also exhibited 5.54 and 2.58 fold increasing in the AUC, respectively.
30502425	4	81	theme	precipitation	853:865	arg1	method					867:872	anti-solvent precipitation method	840:872	anti-solvent precipitation method	840:872	Two silybin nanocrystals formulations (PEG-CS nanocrystals and nanocaged nanocrystals) were prepared using anti-solvent precipitation method and optimized by central composite design-response surface model.
30502425	1	82	theme	multifunctional	256:270	arg1	nanocrystals					305:316	multifunctional mPEG-chitosan stabilized silybin nanocrystals	256:316	multifunctional mPEG-chitosan stabilized silybin nanocrystals	256:316	This study explored the feasibility of using smart nanocaged carrier technology for the delivering of multifunctional mPEG-chitosan stabilized silybin nanocrystals and to enhancing the long-term stability, dissolution velocity and bioavailability of the water insoluble drugs.
30087828	9	0	theme	OS	1469:1470	arg1	concentration					1472:1484	the total OS concentration	1459:1484	the total OS concentration	1459:1484	During the first 7 days of lactation, the percentage of neutral and fucosylated OS increased, whereas acidic OS decreased and the total OS concentration ranged from 217.8 mg·L-1 to 79.8 mg·L-1.
30087828	8	1	theme	mammal	1319:1324	arg1	milks					1326:1330	other mammal milks	1313:1330	other mammal milks	1313:1330	Additionally, there were seven unique OS not previously found in other mammal milks.
30087828	9	2	theme	lactation	1360:1368	arg1	7 days					1350:1355	the first 7 days	1340:1355	the first 7 days of lactation	1340:1368	During the first 7 days of lactation, the percentage of neutral and fucosylated OS increased, whereas acidic OS decreased and the total OS concentration ranged from 217.8 mg·L-1 to 79.8 mg·L-1.
30087828	7	3	theme	mammalian	1048:1056	arg1	milks					1058:1062	other well-characterized mammalian milks	1023:1062	other well-characterized mammalian milks	1023:1062	Comparison with other well-characterized mammalian milks revealed that mare's milk shared 8 OS structures with human, bovine, pig and goat milk (i.e., 2 sialyllactose isomers, 3 hexose, LNH, LNT, and OS with the composition 3 Hex-1 Neu5Ac).
30087828	9	4	theme	acidic	1435:1440	arg1	OS					1442:1443	acidic OS	1435:1443	acidic OS	1435:1443	During the first 7 days of lactation, the percentage of neutral and fucosylated OS increased, whereas acidic OS decreased and the total OS concentration ranged from 217.8 mg·L-1 to 79.8 mg·L-1.
30087828	0	5	theme	oligosaccharide	60:74	arg1	profile					76:82	a unique and diverse free oligosaccharide profile	34:82	a unique and diverse free oligosaccharide profile	34:82	Thoroughbred mare's milk exhibits a unique and diverse free oligosaccharide profile.
30087828	8	6	located	found	1304:1308	arg1	milks					1326:1330	other mammal milks	1313:1330	other mammal milks	1313:1330	Additionally, there were seven unique OS not previously found in other mammal milks.
30087828	8	6	located	found	1304:1308	arg2	OS					1286:1287	seven unique OS	1273:1287	seven unique OS not previously found in other mammal milks	1273:1330	Additionally, there were seven unique OS not previously found in other mammal milks.
30087828	6	7	gly	fucosylated	930:940	arg1	structures					945:954	fucosylated OS structures	930:954	fucosylated OS structures (6.25%)	930:962	Neutral OS were the most abundant glycans (58.3%), followed by acidic OS containing Neu5Ac (33.3%), a minor presence of fucosylated OS structures (6.25%) and one structure containing NeuGc (2.1%).
30087828	6	7	gly	fucosylated	930:940	arg1	%					961:961	6.25%	957:961	6.25%	957:961	Neutral OS were the most abundant glycans (58.3%), followed by acidic OS containing Neu5Ac (33.3%), a minor presence of fucosylated OS structures (6.25%) and one structure containing NeuGc (2.1%).
30087828	6	8	theme	minor	912:916	arg1	presence					918:925	a minor presence	910:925	a minor presence of fucosylated OS structures (6.25%)	910:962	Neutral OS were the most abundant glycans (58.3%), followed by acidic OS containing Neu5Ac (33.3%), a minor presence of fucosylated OS structures (6.25%) and one structure containing NeuGc (2.1%).
30087828	9	9	gly	fucosylated	1401:1411	arg1	OS					1413:1414	neutral and fucosylated OS	1389:1414	neutral and fucosylated OS	1389:1414	During the first 7 days of lactation, the percentage of neutral and fucosylated OS increased, whereas acidic OS decreased and the total OS concentration ranged from 217.8 mg·L-1 to 79.8 mg·L-1.
30087828	2	10	theme	foals	239:243	arg1	health					220:225	the health	216:225	the health of neonatal foals	216:243	Mare's milk plays a major role in the health of neonatal foals.
30087828	6	11	contain	containing	883:892	arg2	%					906:906	33.3%	902:906	33.3%	902:906	Neutral OS were the most abundant glycans (58.3%), followed by acidic OS containing Neu5Ac (33.3%), a minor presence of fucosylated OS structures (6.25%) and one structure containing NeuGc (2.1%).
30087828	6	11	contain	containing	883:892	arg2	%					1003:1003	2.1%	1000:1003	2.1%	1000:1003	Neutral OS were the most abundant glycans (58.3%), followed by acidic OS containing Neu5Ac (33.3%), a minor presence of fucosylated OS structures (6.25%) and one structure containing NeuGc (2.1%).
30087828	6	11	contain	containing	883:892	arg2	Neu5Ac					894:899	Neu5Ac	894:899	Neu5Ac (33.3%)	894:907	Neutral OS were the most abundant glycans (58.3%), followed by acidic OS containing Neu5Ac (33.3%), a minor presence of fucosylated OS structures (6.25%) and one structure containing NeuGc (2.1%).
30087828	6	11	contain	containing	883:892	arg1	OS					880:881	acidic OS	873:881	acidic OS containing Neu5Ac (33.3%), a minor presence of fucosylated OS structures (6.25%) and one structure containing NeuGc (2.1%)	873:1004	Neutral OS were the most abundant glycans (58.3%), followed by acidic OS containing Neu5Ac (33.3%), a minor presence of fucosylated OS structures (6.25%) and one structure containing NeuGc (2.1%).
30087828	6	11	contain	containing	883:892	arg2	NeuGc					993:997	one structure containing NeuGc	968:997	one structure containing NeuGc (2.1%)	968:1004	Neutral OS were the most abundant glycans (58.3%), followed by acidic OS containing Neu5Ac (33.3%), a minor presence of fucosylated OS structures (6.25%) and one structure containing NeuGc (2.1%).
30087828	6	11	contain	containing	883:892	arg2	presence					918:925	a minor presence	910:925	a minor presence of fucosylated OS structures (6.25%)	910:962	Neutral OS were the most abundant glycans (58.3%), followed by acidic OS containing Neu5Ac (33.3%), a minor presence of fucosylated OS structures (6.25%) and one structure containing NeuGc (2.1%).
30087828	2	12	theme	neonatal	230:237	arg1	foals					239:243	neonatal foals	230:243	neonatal foals	230:243	Mare's milk plays a major role in the health of neonatal foals.
30087828	6	13	theme	OS	942:943	arg1	structures					945:954	fucosylated OS structures	930:954	fucosylated OS structures (6.25%)	930:962	Neutral OS were the most abundant glycans (58.3%), followed by acidic OS containing Neu5Ac (33.3%), a minor presence of fucosylated OS structures (6.25%) and one structure containing NeuGc (2.1%).
30087828	6	13	theme	OS	942:943	arg1	%					961:961	6.25%	957:961	6.25%	957:961	Neutral OS were the most abundant glycans (58.3%), followed by acidic OS containing Neu5Ac (33.3%), a minor presence of fucosylated OS structures (6.25%) and one structure containing NeuGc (2.1%).
30087828	3	14	theme	milk	285:288	arg1	components					264:273	the main components	255:273	the main components of mare's milk	255:288	Although the main components of mare's milk are broadly characterized, free oligosaccharides (OS), which possess various bioactivities in many mammalian milks, have not been fully profiled in Thoroughbreds.
30087828	6	15	theme	containing	982:991	arg1	NeuGc					993:997	one structure containing NeuGc	968:997	one structure containing NeuGc (2.1%)	968:1004	Neutral OS were the most abundant glycans (58.3%), followed by acidic OS containing Neu5Ac (33.3%), a minor presence of fucosylated OS structures (6.25%) and one structure containing NeuGc (2.1%).
30087828	6	15	theme	containing	982:991	arg1	%					1003:1003	2.1%	1000:1003	2.1%	1000:1003	Neutral OS were the most abundant glycans (58.3%), followed by acidic OS containing Neu5Ac (33.3%), a minor presence of fucosylated OS structures (6.25%) and one structure containing NeuGc (2.1%).
30087828	4	16	theme	lactation	560:568	arg1	week					552:555	the first week	542:555	the first week of lactation	542:568	The aim of this study was to identify and quantify OS in Thoroughbred mare's milk during the first week of lactation, when foals typically consume mare's milk exclusively.
30087828	9	17	theme	neutral	1389:1395	arg1	OS					1413:1414	neutral and fucosylated OS	1389:1414	neutral and fucosylated OS	1389:1414	During the first 7 days of lactation, the percentage of neutral and fucosylated OS increased, whereas acidic OS decreased and the total OS concentration ranged from 217.8 mg·L-1 to 79.8 mg·L-1.
30087828	6	18	theme	structure	972:980	arg1	NeuGc					993:997	one structure containing NeuGc	968:997	one structure containing NeuGc (2.1%)	968:1004	Neutral OS were the most abundant glycans (58.3%), followed by acidic OS containing Neu5Ac (33.3%), a minor presence of fucosylated OS structures (6.25%) and one structure containing NeuGc (2.1%).
30087828	6	18	theme	structure	972:980	arg1	%					1003:1003	2.1%	1000:1003	2.1%	1000:1003	Neutral OS were the most abundant glycans (58.3%), followed by acidic OS containing Neu5Ac (33.3%), a minor presence of fucosylated OS structures (6.25%) and one structure containing NeuGc (2.1%).
30087828	5	19	theme	OS	639:640	arg1	anomers					676:682	anomers	676:682	anomers	676:682	A total of 48 OS structures (including isomers and anomers), corresponding to 20 unique compositions, were identified by nano LC-Chip QToF-MS and confirmed by tandem mass spectrometry.
30087828	5	19	theme	OS	639:640	arg1	structures					642:651	48 OS structures	636:651	48 OS structures (including isomers and anomers)	636:683	A total of 48 OS structures (including isomers and anomers), corresponding to 20 unique compositions, were identified by nano LC-Chip QToF-MS and confirmed by tandem mass spectrometry.
30087828	5	19	theme	OS	639:640	arg1	isomers					664:670	isomers	664:670	isomers	664:670	A total of 48 OS structures (including isomers and anomers), corresponding to 20 unique compositions, were identified by nano LC-Chip QToF-MS and confirmed by tandem mass spectrometry.
30087828	5	20	theme	nano	746:749	arg1	QToF-MS					759:765	nano LC-Chip QToF-MS	746:765	nano LC-Chip QToF-MS	746:765	A total of 48 OS structures (including isomers and anomers), corresponding to 20 unique compositions, were identified by nano LC-Chip QToF-MS and confirmed by tandem mass spectrometry.
30087828	0	21	theme	Thoroughbred	0:11	arg1	milk					20:23	Thoroughbred mare's milk	0:23	Thoroughbred mare's milk	0:23	Thoroughbred mare's milk exhibits a unique and diverse free oligosaccharide profile.
30087828	8	22	theme	unique	1279:1284	arg1	OS					1286:1287	seven unique OS	1273:1287	seven unique OS not previously found in other mammal milks	1273:1330	Additionally, there were seven unique OS not previously found in other mammal milks.
30087828	9	23	theme	fucosylated	1401:1411	arg1	OS					1413:1414	neutral and fucosylated OS	1389:1414	neutral and fucosylated OS	1389:1414	During the first 7 days of lactation, the percentage of neutral and fucosylated OS increased, whereas acidic OS decreased and the total OS concentration ranged from 217.8 mg·L-1 to 79.8 mg·L-1.
30087828	3	24	theme	various	359:365	arg1	bioactivities					367:379	various bioactivities	359:379	various bioactivities in many mammalian milks	359:403	Although the main components of mare's milk are broadly characterized, free oligosaccharides (OS), which possess various bioactivities in many mammalian milks, have not been fully profiled in Thoroughbreds.
30087828	4	25	theme	study	469:473	arg1	aim					457:459	The aim	453:459	The aim of this study	453:473	The aim of this study was to identify and quantify OS in Thoroughbred mare's milk during the first week of lactation, when foals typically consume mare's milk exclusively.
30087828	9	26	theme	OS	1413:1414	arg1	percentage					1375:1384	the percentage	1371:1384	the percentage of neutral and fucosylated OS	1371:1414	During the first 7 days of lactation, the percentage of neutral and fucosylated OS increased, whereas acidic OS decreased and the total OS concentration ranged from 217.8 mg·L-1 to 79.8 mg·L-1.
30087828	7	27	theme	sialyllactose	1160:1172	arg1	LNT					1198:1200	LNT	1198:1200	LNT	1198:1200	Comparison with other well-characterized mammalian milks revealed that mare's milk shared 8 OS structures with human, bovine, pig and goat milk (i.e., 2 sialyllactose isomers, 3 hexose, LNH, LNT, and OS with the composition 3 Hex-1 Neu5Ac).
30087828	7	27	theme	sialyllactose	1160:1172	arg1	OS					1207:1208	OS	1207:1208	OS	1207:1208	Comparison with other well-characterized mammalian milks revealed that mare's milk shared 8 OS structures with human, bovine, pig and goat milk (i.e., 2 sialyllactose isomers, 3 hexose, LNH, LNT, and OS with the composition 3 Hex-1 Neu5Ac).
30087828	7	27	theme	sialyllactose	1160:1172	arg1	LNH					1193:1195	LNH	1193:1195	LNH	1193:1195	Comparison with other well-characterized mammalian milks revealed that mare's milk shared 8 OS structures with human, bovine, pig and goat milk (i.e., 2 sialyllactose isomers, 3 hexose, LNH, LNT, and OS with the composition 3 Hex-1 Neu5Ac).
30087828	7	27	theme	sialyllactose	1160:1172	arg1	isomers					1174:1180	2 sialyllactose isomers	1158:1180	2 sialyllactose isomers	1158:1180	Comparison with other well-characterized mammalian milks revealed that mare's milk shared 8 OS structures with human, bovine, pig and goat milk (i.e., 2 sialyllactose isomers, 3 hexose, LNH, LNT, and OS with the composition 3 Hex-1 Neu5Ac).
30087828	7	27	theme	sialyllactose	1160:1172	arg1	hexose					1185:1190	3 hexose	1183:1190	3 hexose	1183:1190	Comparison with other well-characterized mammalian milks revealed that mare's milk shared 8 OS structures with human, bovine, pig and goat milk (i.e., 2 sialyllactose isomers, 3 hexose, LNH, LNT, and OS with the composition 3 Hex-1 Neu5Ac).
30087828	1	28	theme	major	166:170	arg1	industry					172:179	a major industry	164:179	a major industry	164:179	The Thoroughbred is among the most valuable horse breeds, and its husbandry is a major industry.
30087828	1	28	theme	major	166:170	arg1	husbandry					151:159	its husbandry	147:159	its husbandry	147:159	The Thoroughbred is among the most valuable horse breeds, and its husbandry is a major industry.
30087828	3	29	theme	free	317:320	arg1	oligosaccharides					322:337	free oligosaccharides	317:337	free oligosaccharides (OS)	317:342	Although the main components of mare's milk are broadly characterized, free oligosaccharides (OS), which possess various bioactivities in many mammalian milks, have not been fully profiled in Thoroughbreds.
30087828	3	29	theme	free	317:320	arg1	OS					340:341	OS	340:341	OS	340:341	Although the main components of mare's milk are broadly characterized, free oligosaccharides (OS), which possess various bioactivities in many mammalian milks, have not been fully profiled in Thoroughbreds.
30087828	6	30	theme	abundant	835:842	arg1	glycans					844:850	the most abundant glycans	826:850	the most abundant glycans (58.3%)	826:858	Neutral OS were the most abundant glycans (58.3%), followed by acidic OS containing Neu5Ac (33.3%), a minor presence of fucosylated OS structures (6.25%) and one structure containing NeuGc (2.1%).
30087828	6	30	theme	abundant	835:842	arg1	%					857:857	58.3%	853:857	58.3%	853:857	Neutral OS were the most abundant glycans (58.3%), followed by acidic OS containing Neu5Ac (33.3%), a minor presence of fucosylated OS structures (6.25%) and one structure containing NeuGc (2.1%).
30087828	6	30	theme	abundant	835:842	arg1	OS					818:819	Neutral OS	810:819	Neutral OS	810:819	Neutral OS were the most abundant glycans (58.3%), followed by acidic OS containing Neu5Ac (33.3%), a minor presence of fucosylated OS structures (6.25%) and one structure containing NeuGc (2.1%).
30087828	6	31	theme	fucosylated	930:940	arg1	structures					945:954	fucosylated OS structures	930:954	fucosylated OS structures (6.25%)	930:962	Neutral OS were the most abundant glycans (58.3%), followed by acidic OS containing Neu5Ac (33.3%), a minor presence of fucosylated OS structures (6.25%) and one structure containing NeuGc (2.1%).
30087828	6	31	theme	fucosylated	930:940	arg1	%					961:961	6.25%	957:961	6.25%	957:961	Neutral OS were the most abundant glycans (58.3%), followed by acidic OS containing Neu5Ac (33.3%), a minor presence of fucosylated OS structures (6.25%) and one structure containing NeuGc (2.1%).
30087828	7	32	theme	OS	1099:1100	arg1	structures					1102:1111	8 OS structures	1097:1111	8 OS structures with human, bovine, pig and goat milk (i.e., 2 sialyllactose isomers, 3 hexose, LNH, LNT, and OS with the composition 3 Hex-1 Neu5Ac)	1097:1245	Comparison with other well-characterized mammalian milks revealed that mare's milk shared 8 OS structures with human, bovine, pig and goat milk (i.e., 2 sialyllactose isomers, 3 hexose, LNH, LNT, and OS with the composition 3 Hex-1 Neu5Ac).
30087828	7	33	with	structures	1102:1111	arg1	goat					1141:1144	goat	1141:1144	goat	1141:1144	Comparison with other well-characterized mammalian milks revealed that mare's milk shared 8 OS structures with human, bovine, pig and goat milk (i.e., 2 sialyllactose isomers, 3 hexose, LNH, LNT, and OS with the composition 3 Hex-1 Neu5Ac).
30087828	7	33	with	structures	1102:1111	arg1	pig					1133:1135	pig	1133:1135	pig	1133:1135	Comparison with other well-characterized mammalian milks revealed that mare's milk shared 8 OS structures with human, bovine, pig and goat milk (i.e., 2 sialyllactose isomers, 3 hexose, LNH, LNT, and OS with the composition 3 Hex-1 Neu5Ac).
30087828	7	33	with	structures	1102:1111	arg1	bovine					1125:1130	bovine	1125:1130	bovine	1125:1130	Comparison with other well-characterized mammalian milks revealed that mare's milk shared 8 OS structures with human, bovine, pig and goat milk (i.e., 2 sialyllactose isomers, 3 hexose, LNH, LNT, and OS with the composition 3 Hex-1 Neu5Ac).
30087828	7	33	with	structures	1102:1111	arg1	human					1118:1122	human	1118:1122	human	1118:1122	Comparison with other well-characterized mammalian milks revealed that mare's milk shared 8 OS structures with human, bovine, pig and goat milk (i.e., 2 sialyllactose isomers, 3 hexose, LNH, LNT, and OS with the composition 3 Hex-1 Neu5Ac).
30087828	7	34	dep	human	1118:1122	arg1	milk					1146:1149	milk	1146:1149	milk	1146:1149	Comparison with other well-characterized mammalian milks revealed that mare's milk shared 8 OS structures with human, bovine, pig and goat milk (i.e., 2 sialyllactose isomers, 3 hexose, LNH, LNT, and OS with the composition 3 Hex-1 Neu5Ac).
30087828	5	35	theme	LC-Chip	751:757	arg1	QToF-MS					759:765	nano LC-Chip QToF-MS	746:765	nano LC-Chip QToF-MS	746:765	A total of 48 OS structures (including isomers and anomers), corresponding to 20 unique compositions, were identified by nano LC-Chip QToF-MS and confirmed by tandem mass spectrometry.
30087828	3	36	theme	many	384:387	arg1	milks					399:403	many mammalian milks	384:403	many mammalian milks	384:403	Although the main components of mare's milk are broadly characterized, free oligosaccharides (OS), which possess various bioactivities in many mammalian milks, have not been fully profiled in Thoroughbreds.
30087828	9	37	theme	total	1463:1467	arg1	concentration					1472:1484	the total OS concentration	1459:1484	the total OS concentration	1459:1484	During the first 7 days of lactation, the percentage of neutral and fucosylated OS increased, whereas acidic OS decreased and the total OS concentration ranged from 217.8 mg·L-1 to 79.8 mg·L-1.
30087828	3	38	theme	mammalian	389:397	arg1	milks					399:403	many mammalian milks	384:403	many mammalian milks	384:403	Although the main components of mare's milk are broadly characterized, free oligosaccharides (OS), which possess various bioactivities in many mammalian milks, have not been fully profiled in Thoroughbreds.
30087828	7	39	theme	other	1023:1027	arg1	milks					1058:1062	other well-characterized mammalian milks	1023:1062	other well-characterized mammalian milks	1023:1062	Comparison with other well-characterized mammalian milks revealed that mare's milk shared 8 OS structures with human, bovine, pig and goat milk (i.e., 2 sialyllactose isomers, 3 hexose, LNH, LNT, and OS with the composition 3 Hex-1 Neu5Ac).
30087828	5	40	theme	structures	642:651	arg1	total					627:631	A total	625:631	A total	625:631	A total of 48 OS structures (including isomers and anomers), corresponding to 20 unique compositions, were identified by nano LC-Chip QToF-MS and confirmed by tandem mass spectrometry.
30087828	7	41	with	isomers	1174:1180	arg1	Neu5Ac					1239:1244	the composition 3 Hex-1 Neu5Ac	1215:1244	the composition 3 Hex-1 Neu5Ac	1215:1244	Comparison with other well-characterized mammalian milks revealed that mare's milk shared 8 OS structures with human, bovine, pig and goat milk (i.e., 2 sialyllactose isomers, 3 hexose, LNH, LNT, and OS with the composition 3 Hex-1 Neu5Ac).
30087828	5	42	theme	tandem	784:789	arg1	spectrometry					796:807	tandem mass spectrometry	784:807	tandem mass spectrometry	784:807	A total of 48 OS structures (including isomers and anomers), corresponding to 20 unique compositions, were identified by nano LC-Chip QToF-MS and confirmed by tandem mass spectrometry.
30087828	0	43	theme	unique	36:41	arg1	profile					76:82	a unique and diverse free oligosaccharide profile	34:82	a unique and diverse free oligosaccharide profile	34:82	Thoroughbred mare's milk exhibits a unique and diverse free oligosaccharide profile.
30087828	8	44	theme	other	1313:1317	arg1	milks					1326:1330	other mammal milks	1313:1330	other mammal milks	1313:1330	Additionally, there were seven unique OS not previously found in other mammal milks.
30087828	3	45	theme	main	259:262	arg1	components					264:273	the main components	255:273	the main components of mare's milk	255:288	Although the main components of mare's milk are broadly characterized, free oligosaccharides (OS), which possess various bioactivities in many mammalian milks, have not been fully profiled in Thoroughbreds.
30087828	5	46	theme	mass	791:794	arg1	spectrometry					796:807	tandem mass spectrometry	784:807	tandem mass spectrometry	784:807	A total of 48 OS structures (including isomers and anomers), corresponding to 20 unique compositions, were identified by nano LC-Chip QToF-MS and confirmed by tandem mass spectrometry.
30087828	6	47	theme	Neutral	810:816	arg1	glycans					844:850	the most abundant glycans	826:850	the most abundant glycans (58.3%)	826:858	Neutral OS were the most abundant glycans (58.3%), followed by acidic OS containing Neu5Ac (33.3%), a minor presence of fucosylated OS structures (6.25%) and one structure containing NeuGc (2.1%).
30087828	6	47	theme	Neutral	810:816	arg1	OS					818:819	Neutral OS	810:819	Neutral OS	810:819	Neutral OS were the most abundant glycans (58.3%), followed by acidic OS containing Neu5Ac (33.3%), a minor presence of fucosylated OS structures (6.25%) and one structure containing NeuGc (2.1%).
30087828	3	48	contain	possess	351:357	arg1	oligosaccharides					322:337	free oligosaccharides	317:337	free oligosaccharides (OS)	317:342	Although the main components of mare's milk are broadly characterized, free oligosaccharides (OS), which possess various bioactivities in many mammalian milks, have not been fully profiled in Thoroughbreds.
30087828	3	48	contain	possess	351:357	arg1	OS					340:341	OS	340:341	OS	340:341	Although the main components of mare's milk are broadly characterized, free oligosaccharides (OS), which possess various bioactivities in many mammalian milks, have not been fully profiled in Thoroughbreds.
30087828	3	48	contain	possess	351:357	arg2	bioactivities					367:379	various bioactivities	359:379	various bioactivities in many mammalian milks	359:403	Although the main components of mare's milk are broadly characterized, free oligosaccharides (OS), which possess various bioactivities in many mammalian milks, have not been fully profiled in Thoroughbreds.
30087828	7	49	with	Comparison	1007:1016	arg1	milks					1058:1062	other well-characterized mammalian milks	1023:1062	other well-characterized mammalian milks	1023:1062	Comparison with other well-characterized mammalian milks revealed that mare's milk shared 8 OS structures with human, bovine, pig and goat milk (i.e., 2 sialyllactose isomers, 3 hexose, LNH, LNT, and OS with the composition 3 Hex-1 Neu5Ac).
30087828	6	50	theme	acidic	873:878	arg1	OS					880:881	acidic OS	873:881	acidic OS containing Neu5Ac (33.3%), a minor presence of fucosylated OS structures (6.25%) and one structure containing NeuGc (2.1%)	873:1004	Neutral OS were the most abundant glycans (58.3%), followed by acidic OS containing Neu5Ac (33.3%), a minor presence of fucosylated OS structures (6.25%) and one structure containing NeuGc (2.1%).
30087828	6	51	theme	structures	945:954	arg1	%					1003:1003	2.1%	1000:1003	2.1%	1000:1003	Neutral OS were the most abundant glycans (58.3%), followed by acidic OS containing Neu5Ac (33.3%), a minor presence of fucosylated OS structures (6.25%) and one structure containing NeuGc (2.1%).
30087828	6	51	theme	structures	945:954	arg1	NeuGc					993:997	one structure containing NeuGc	968:997	one structure containing NeuGc (2.1%)	968:1004	Neutral OS were the most abundant glycans (58.3%), followed by acidic OS containing Neu5Ac (33.3%), a minor presence of fucosylated OS structures (6.25%) and one structure containing NeuGc (2.1%).
30087828	6	51	theme	structures	945:954	arg1	Neu5Ac					894:899	Neu5Ac	894:899	Neu5Ac (33.3%)	894:907	Neutral OS were the most abundant glycans (58.3%), followed by acidic OS containing Neu5Ac (33.3%), a minor presence of fucosylated OS structures (6.25%) and one structure containing NeuGc (2.1%).
30087828	6	51	theme	structures	945:954	arg1	%					906:906	33.3%	902:906	33.3%	902:906	Neutral OS were the most abundant glycans (58.3%), followed by acidic OS containing Neu5Ac (33.3%), a minor presence of fucosylated OS structures (6.25%) and one structure containing NeuGc (2.1%).
30087828	6	51	theme	structures	945:954	arg1	presence					918:925	a minor presence	910:925	a minor presence of fucosylated OS structures (6.25%)	910:962	Neutral OS were the most abundant glycans (58.3%), followed by acidic OS containing Neu5Ac (33.3%), a minor presence of fucosylated OS structures (6.25%) and one structure containing NeuGc (2.1%).
30087828	7	52	theme	Hex-1	1233:1237	arg1	Neu5Ac					1239:1244	the composition 3 Hex-1 Neu5Ac	1215:1244	the composition 3 Hex-1 Neu5Ac	1215:1244	Comparison with other well-characterized mammalian milks revealed that mare's milk shared 8 OS structures with human, bovine, pig and goat milk (i.e., 2 sialyllactose isomers, 3 hexose, LNH, LNT, and OS with the composition 3 Hex-1 Neu5Ac).
30087828	9	53	theme	first	1344:1348	arg1	7 days					1350:1355	the first 7 days	1340:1355	the first 7 days of lactation	1340:1368	During the first 7 days of lactation, the percentage of neutral and fucosylated OS increased, whereas acidic OS decreased and the total OS concentration ranged from 217.8 mg·L-1 to 79.8 mg·L-1.
30087828	1	54	theme	valuable	120:127	arg1	breeds					135:140	the most valuable horse breeds	111:140	the most valuable horse breeds	111:140	The Thoroughbred is among the most valuable horse breeds, and its husbandry is a major industry.
30087828	4	55	theme	Thoroughbred	510:521	arg1	milk					530:533	Thoroughbred mare's milk	510:533	Thoroughbred mare's milk	510:533	The aim of this study was to identify and quantify OS in Thoroughbred mare's milk during the first week of lactation, when foals typically consume mare's milk exclusively.
30087828	0	56	theme	free	55:58	arg1	profile					76:82	a unique and diverse free oligosaccharide profile	34:82	a unique and diverse free oligosaccharide profile	34:82	Thoroughbred mare's milk exhibits a unique and diverse free oligosaccharide profile.
30087828	4	57	theme	first	546:550	arg1	week					552:555	the first week	542:555	the first week of lactation	542:568	The aim of this study was to identify and quantify OS in Thoroughbred mare's milk during the first week of lactation, when foals typically consume mare's milk exclusively.
30087828	7	58	dep	structures	1102:1111	arg1	LNT					1198:1200	LNT	1198:1200	LNT	1198:1200	Comparison with other well-characterized mammalian milks revealed that mare's milk shared 8 OS structures with human, bovine, pig and goat milk (i.e., 2 sialyllactose isomers, 3 hexose, LNH, LNT, and OS with the composition 3 Hex-1 Neu5Ac).
30087828	7	58	dep	structures	1102:1111	arg1	OS					1207:1208	OS	1207:1208	OS	1207:1208	Comparison with other well-characterized mammalian milks revealed that mare's milk shared 8 OS structures with human, bovine, pig and goat milk (i.e., 2 sialyllactose isomers, 3 hexose, LNH, LNT, and OS with the composition 3 Hex-1 Neu5Ac).
30087828	7	58	dep	structures	1102:1111	arg1	LNH					1193:1195	LNH	1193:1195	LNH	1193:1195	Comparison with other well-characterized mammalian milks revealed that mare's milk shared 8 OS structures with human, bovine, pig and goat milk (i.e., 2 sialyllactose isomers, 3 hexose, LNH, LNT, and OS with the composition 3 Hex-1 Neu5Ac).
30087828	7	58	dep	structures	1102:1111	arg1	isomers					1174:1180	2 sialyllactose isomers	1158:1180	2 sialyllactose isomers	1158:1180	Comparison with other well-characterized mammalian milks revealed that mare's milk shared 8 OS structures with human, bovine, pig and goat milk (i.e., 2 sialyllactose isomers, 3 hexose, LNH, LNT, and OS with the composition 3 Hex-1 Neu5Ac).
30087828	7	58	dep	structures	1102:1111	arg1	hexose					1185:1190	3 hexose	1183:1190	3 hexose	1183:1190	Comparison with other well-characterized mammalian milks revealed that mare's milk shared 8 OS structures with human, bovine, pig and goat milk (i.e., 2 sialyllactose isomers, 3 hexose, LNH, LNT, and OS with the composition 3 Hex-1 Neu5Ac).
30087828	5	59	theme	unique	706:711	arg1	compositions					713:724	20 unique compositions	703:724	20 unique compositions	703:724	A total of 48 OS structures (including isomers and anomers), corresponding to 20 unique compositions, were identified by nano LC-Chip QToF-MS and confirmed by tandem mass spectrometry.
30087828	1	60	theme	horse	129:133	arg1	breeds					135:140	the most valuable horse breeds	111:140	the most valuable horse breeds	111:140	The Thoroughbred is among the most valuable horse breeds, and its husbandry is a major industry.
30087828	7	61	theme	well-characterized	1029:1046	arg1	milks					1058:1062	other well-characterized mammalian milks	1023:1062	other well-characterized mammalian milks	1023:1062	Comparison with other well-characterized mammalian milks revealed that mare's milk shared 8 OS structures with human, bovine, pig and goat milk (i.e., 2 sialyllactose isomers, 3 hexose, LNH, LNT, and OS with the composition 3 Hex-1 Neu5Ac).
30087828	0	62	theme	diverse	47:53	arg1	profile					76:82	a unique and diverse free oligosaccharide profile	34:82	a unique and diverse free oligosaccharide profile	34:82	Thoroughbred mare's milk exhibits a unique and diverse free oligosaccharide profile.
30087828	7	63	theme	composition	1219:1229	arg1	Neu5Ac					1239:1244	the composition 3 Hex-1 Neu5Ac	1215:1244	the composition 3 Hex-1 Neu5Ac	1215:1244	Comparison with other well-characterized mammalian milks revealed that mare's milk shared 8 OS structures with human, bovine, pig and goat milk (i.e., 2 sialyllactose isomers, 3 hexose, LNH, LNT, and OS with the composition 3 Hex-1 Neu5Ac).
30087828	2	64	theme	major	202:206	arg1	role					208:211	a major role	200:211	a major role	200:211	Mare's milk plays a major role in the health of neonatal foals.
30087828	3	65	from	bioactivities	367:379	arg1	milks					399:403	many mammalian milks	384:403	many mammalian milks	384:403	Although the main components of mare's milk are broadly characterized, free oligosaccharides (OS), which possess various bioactivities in many mammalian milks, have not been fully profiled in Thoroughbreds.
30087828	7	66	dep	isomers	1174:1180	arg1	i.e.					1152:1155	i.e.	1152:1155	i.e.	1152:1155	Comparison with other well-characterized mammalian milks revealed that mare's milk shared 8 OS structures with human, bovine, pig and goat milk (i.e., 2 sialyllactose isomers, 3 hexose, LNH, LNT, and OS with the composition 3 Hex-1 Neu5Ac).
29736686	7	0	theme	incubation	1209:1218	arg1	time					1220:1223	long incubation time	1204:1223	long incubation time	1204:1223	At long incubation time no significant difference among the biomineralized and BMP-activated scaffolds was observed.
29736686	7	1	located	observed	1308:1315	arg1	time					1220:1223	long incubation time	1204:1223	long incubation time	1204:1223	At long incubation time no significant difference among the biomineralized and BMP-activated scaffolds was observed.
29736686	7	1	located	observed	1308:1315	arg2	difference					1240:1249	no significant difference	1225:1249	no significant difference among the biomineralized and BMP-activated scaffolds	1225:1302	At long incubation time no significant difference among the biomineralized and BMP-activated scaffolds was observed.
29736686	2	2	from	sequence	448:455	arg1	epitope					468:474	knuckle epitope	460:474	knuckle epitope of BMP-2	460:483	The first approach is based on the use of a sequence of 20 amino acids corresponding to a 68-87 sequence in knuckle epitope of BMP-2 that was coupled covalently to the carboxyl group of chitosan scaffold.
29736686	0	3	theme	biological	68:77	arg1	performance					79:89	the biological performance	64:89	the biological performance of chitosan	64:101	Effect of inorganic and organic bioactive signals decoration on the biological performance of chitosan scaffolds for bone tissue engineering.
29736686	2	4	theme	first	356:360	arg1	approach					362:369	The first approach	352:369	The first approach	352:369	The first approach is based on the use of a sequence of 20 amino acids corresponding to a 68-87 sequence in knuckle epitope of BMP-2 that was coupled covalently to the carboxyl group of chitosan scaffold.
29736686	1	5	theme	inorganic	255:263	arg1	signals					265:271	organic and inorganic signals	243:271	organic and inorganic signals	243:271	The present work is focused on the design of a bioactive chitosan-based scaffold functionalized with organic and inorganic signals to provide the biochemical cues for promoting stem cell osteogenic commitment.
29736686	0	6	theme	chitosan	94:101	arg1	performance					79:89	the biological performance	64:89	the biological performance of chitosan	64:101	Effect of inorganic and organic bioactive signals decoration on the biological performance of chitosan scaffolds for bone tissue engineering.
29736686	0	7	from	Effect	0:5	arg1	performance					79:89	the biological performance	64:89	the biological performance of chitosan	64:101	Effect of inorganic and organic bioactive signals decoration on the biological performance of chitosan scaffolds for bone tissue engineering.
29736686	4	8	theme	neat	855:858	arg1	scaffolds					860:868	the neat scaffolds	851:868	the neat scaffolds after 3 days of cell culture	851:897	Both scaffolds bioactivated with organic and inorganic signals induce higher expression of an early marker of osteogenic differentiation (ALP) than the neat scaffolds after 3 days of cell culture.
29736686	6	9	theme	osteogenic	1173:1182	arg1	differentiation					1184:1198	hMSC osteogenic differentiation	1168:1198	hMSC osteogenic differentiation	1168:1198	Nevertheless, the biomineralized scaffolds showed better cellular behaviour than neat scaffolds, demonstrating the good effect of hydroxyapatite deposits on hMSC osteogenic differentiation.
29736686	9	10	from	effect	1557:1562	arg1	hMSC					1567:1570	hMSC	1567:1570	hMSC	1567:1570	The overall results showed that the presence of bioactive signals on the scaffold surface allows an osteoinductive effect on hMSC in a basal medium, making the modified chitosan scaffolds a promising candidate for bone tissue regeneration.
29736686	7	11	theme	long	1204:1207	arg1	time					1220:1223	long incubation time	1204:1223	long incubation time	1204:1223	At long incubation time no significant difference among the biomineralized and BMP-activated scaffolds was observed.
29736686	9	12	theme	signals	1500:1506	arg1	presence					1478:1485	the presence	1474:1485	the presence of bioactive signals on the scaffold surface	1474:1530	The overall results showed that the presence of bioactive signals on the scaffold surface allows an osteoinductive effect on hMSC in a basal medium, making the modified chitosan scaffolds a promising candidate for bone tissue regeneration.
29736686	4	13	theme	organic	736:742	arg1	signals					758:764	organic and inorganic signals	736:764	organic and inorganic signals	736:764	Both scaffolds bioactivated with organic and inorganic signals induce higher expression of an early marker of osteogenic differentiation (ALP) than the neat scaffolds after 3 days of cell culture.
29736686	3	14	theme	scaffold	685:692	arg1	surface					694:700	the scaffold surface	681:700	the scaffold surface	681:700	Meanwhile, the second approach is based on the biomimetic treatment, which allows the formation of hydroxyapatite nuclei on the scaffold surface.
29736686	7	15	theme	BMP-activated	1280:1292	arg1	scaffolds					1294:1302	the biomineralized and BMP-activated scaffolds	1257:1302	the biomineralized and BMP-activated scaffolds	1257:1302	At long incubation time no significant difference among the biomineralized and BMP-activated scaffolds was observed.
29736686	2	16	theme	carboxyl	520:527	arg1	group					529:533	the carboxyl group	516:533	the carboxyl group of chitosan scaffold	516:554	The first approach is based on the use of a sequence of 20 amino acids corresponding to a 68-87 sequence in knuckle epitope of BMP-2 that was coupled covalently to the carboxyl group of chitosan scaffold.
29736686	2	16	theme	carboxyl	520:527	arg1	scaffold					547:554	chitosan scaffold	538:554	chitosan scaffold	538:554	The first approach is based on the use of a sequence of 20 amino acids corresponding to a 68-87 sequence in knuckle epitope of BMP-2 that was coupled covalently to the carboxyl group of chitosan scaffold.
29736686	6	17	theme	better	1061:1066	arg1	behaviour					1077:1085	better cellular behaviour	1061:1085	better cellular behaviour	1061:1085	Nevertheless, the biomineralized scaffolds showed better cellular behaviour than neat scaffolds, demonstrating the good effect of hydroxyapatite deposits on hMSC osteogenic differentiation.
29736686	8	18	theme	highest	1335:1341	arg1	level					1343:1347	the highest level	1331:1347	the highest level of Osteocalcin expression (OCN)	1331:1379	Furthermore, the highest level of Osteocalcin expression (OCN) was observed for scaffold with BMP2 mimic-peptide at day 21.
29736686	4	19	theme	osteogenic	813:822	arg1	ALP					841:843	ALP	841:843	ALP	841:843	Both scaffolds bioactivated with organic and inorganic signals induce higher expression of an early marker of osteogenic differentiation (ALP) than the neat scaffolds after 3 days of cell culture.
29736686	4	19	theme	osteogenic	813:822	arg1	differentiation					824:838	osteogenic differentiation	813:838	osteogenic differentiation (ALP)	813:844	Both scaffolds bioactivated with organic and inorganic signals induce higher expression of an early marker of osteogenic differentiation (ALP) than the neat scaffolds after 3 days of cell culture.
29736686	4	20	theme	inorganic	748:756	arg1	signals					758:764	organic and inorganic signals	736:764	organic and inorganic signals	736:764	Both scaffolds bioactivated with organic and inorganic signals induce higher expression of an early marker of osteogenic differentiation (ALP) than the neat scaffolds after 3 days of cell culture.
29736686	6	21	theme	cellular	1068:1075	arg1	behaviour					1077:1085	better cellular behaviour	1061:1085	better cellular behaviour	1061:1085	Nevertheless, the biomineralized scaffolds showed better cellular behaviour than neat scaffolds, demonstrating the good effect of hydroxyapatite deposits on hMSC osteogenic differentiation.
29736686	9	22	theme	overall	1446:1452	arg1	results					1454:1460	The overall results	1442:1460	The overall results	1442:1460	The overall results showed that the presence of bioactive signals on the scaffold surface allows an osteoinductive effect on hMSC in a basal medium, making the modified chitosan scaffolds a promising candidate for bone tissue regeneration.
29736686	2	23	theme	knuckle	460:466	arg1	epitope					468:474	knuckle epitope	460:474	knuckle epitope of BMP-2	460:483	The first approach is based on the use of a sequence of 20 amino acids corresponding to a 68-87 sequence in knuckle epitope of BMP-2 that was coupled covalently to the carboxyl group of chitosan scaffold.
29736686	6	24	theme	hydroxyapatite	1141:1154	arg1	deposits					1156:1163	hydroxyapatite deposits	1141:1163	hydroxyapatite deposits	1141:1163	Nevertheless, the biomineralized scaffolds showed better cellular behaviour than neat scaffolds, demonstrating the good effect of hydroxyapatite deposits on hMSC osteogenic differentiation.
29736686	3	25	theme	hydroxyapatite	656:669	arg1	nuclei					671:676	hydroxyapatite nuclei	656:676	hydroxyapatite nuclei	656:676	Meanwhile, the second approach is based on the biomimetic treatment, which allows the formation of hydroxyapatite nuclei on the scaffold surface.
29736686	9	26	attach	presence	1478:1485	arg1	surface					1524:1530	the scaffold surface	1511:1530	the scaffold surface	1511:1530	The overall results showed that the presence of bioactive signals on the scaffold surface allows an osteoinductive effect on hMSC in a basal medium, making the modified chitosan scaffolds a promising candidate for bone tissue regeneration.
29736686	9	26	attach	presence	1478:1485	arg2	signals					1500:1506	bioactive signals	1490:1506	bioactive signals	1490:1506	The overall results showed that the presence of bioactive signals on the scaffold surface allows an osteoinductive effect on hMSC in a basal medium, making the modified chitosan scaffolds a promising candidate for bone tissue regeneration.
29736686	4	27	theme	differentiation	824:838	arg1	marker					803:808	an early marker	794:808	an early marker of osteogenic differentiation (ALP)	794:844	Both scaffolds bioactivated with organic and inorganic signals induce higher expression of an early marker of osteogenic differentiation (ALP) than the neat scaffolds after 3 days of cell culture.
29736686	6	28	from	effect	1131:1136	arg1	differentiation					1184:1198	hMSC osteogenic differentiation	1168:1198	hMSC osteogenic differentiation	1168:1198	Nevertheless, the biomineralized scaffolds showed better cellular behaviour than neat scaffolds, demonstrating the good effect of hydroxyapatite deposits on hMSC osteogenic differentiation.
29736686	6	29	theme	biomineralized	1029:1042	arg1	scaffolds					1044:1052	the biomineralized scaffolds	1025:1052	the biomineralized scaffolds	1025:1052	Nevertheless, the biomineralized scaffolds showed better cellular behaviour than neat scaffolds, demonstrating the good effect of hydroxyapatite deposits on hMSC osteogenic differentiation.
29736686	2	30	theme	acids	417:421	arg1	sequence					396:403	a sequence	394:403	a sequence of 20 amino acids corresponding to a 68-87 sequence in knuckle epitope of BMP-2 that was coupled covalently to the carboxyl group of chitosan scaffold	394:554	The first approach is based on the use of a sequence of 20 amino acids corresponding to a 68-87 sequence in knuckle epitope of BMP-2 that was coupled covalently to the carboxyl group of chitosan scaffold.
29736686	0	31	theme	tissue	122:127	arg1	engineering					129:139	bone tissue engineering	117:139	bone tissue engineering	117:139	Effect of inorganic and organic bioactive signals decoration on the biological performance of chitosan scaffolds for bone tissue engineering.
29736686	8	32	theme	BMP2	1412:1415	arg1	mimic-peptide					1417:1429	BMP2 mimic-peptide	1412:1429	BMP2 mimic-peptide at day 21	1412:1439	Furthermore, the highest level of Osteocalcin expression (OCN) was observed for scaffold with BMP2 mimic-peptide at day 21.
29736686	9	33	theme	basal	1577:1581	arg1	medium					1583:1588	a basal medium	1575:1588	a basal medium	1575:1588	The overall results showed that the presence of bioactive signals on the scaffold surface allows an osteoinductive effect on hMSC in a basal medium, making the modified chitosan scaffolds a promising candidate for bone tissue regeneration.
29736686	2	34	theme	amino	411:415	arg1	acids					417:421	20 amino acids	408:421	20 amino acids corresponding to a 68-87 sequence in knuckle epitope of BMP-2 that was coupled covalently to the carboxyl group of chitosan scaffold	408:554	The first approach is based on the use of a sequence of 20 amino acids corresponding to a 68-87 sequence in knuckle epitope of BMP-2 that was coupled covalently to the carboxyl group of chitosan scaffold.
29736686	1	35	theme	biochemical	288:298	arg1	cues					300:303	the biochemical cues	284:303	the biochemical cues for promoting stem cell osteogenic commitment	284:349	The present work is focused on the design of a bioactive chitosan-based scaffold functionalized with organic and inorganic signals to provide the biochemical cues for promoting stem cell osteogenic commitment.
29736686	0	36	theme	bone	117:120	arg1	engineering					129:139	bone tissue engineering	117:139	bone tissue engineering	117:139	Effect of inorganic and organic bioactive signals decoration on the biological performance of chitosan scaffolds for bone tissue engineering.
29736686	6	37	theme	good	1126:1129	arg1	effect					1131:1136	the good effect	1122:1136	the good effect of hydroxyapatite deposits on hMSC osteogenic differentiation	1122:1198	Nevertheless, the biomineralized scaffolds showed better cellular behaviour than neat scaffolds, demonstrating the good effect of hydroxyapatite deposits on hMSC osteogenic differentiation.
29736686	8	38	from	day	1434:1436	arg1	mimic-peptide					1417:1429	BMP2 mimic-peptide	1412:1429	BMP2 mimic-peptide at day 21	1412:1439	Furthermore, the highest level of Osteocalcin expression (OCN) was observed for scaffold with BMP2 mimic-peptide at day 21.
29736686	5	39	theme	BMP-mimicking	934:946	arg1	peptide					948:954	BMP-mimicking peptide	934:954	BMP-mimicking peptide	934:954	However, scaffolds decorated with BMP-mimicking peptide show higher values of ALP than the biomineralized one.
29736686	0	40	theme	bioactive	32:40	arg1	decoration					50:59	inorganic and organic bioactive signals decoration	10:59	inorganic and organic bioactive signals decoration	10:59	Effect of inorganic and organic bioactive signals decoration on the biological performance of chitosan scaffolds for bone tissue engineering.
29736686	5	41	theme	ALP	978:980	arg1	values					968:973	higher values	961:973	higher values of ALP	961:980	However, scaffolds decorated with BMP-mimicking peptide show higher values of ALP than the biomineralized one.
29736686	3	42	from	formation	643:651	arg1	surface					694:700	the scaffold surface	681:700	the scaffold surface	681:700	Meanwhile, the second approach is based on the biomimetic treatment, which allows the formation of hydroxyapatite nuclei on the scaffold surface.
29736686	2	43	theme	sequence	396:403	arg1	use					387:389	the use	383:389	the use of a sequence of 20 amino acids corresponding to a 68-87 sequence in knuckle epitope of BMP-2 that was coupled covalently to the carboxyl group of chitosan scaffold	383:554	The first approach is based on the use of a sequence of 20 amino acids corresponding to a 68-87 sequence in knuckle epitope of BMP-2 that was coupled covalently to the carboxyl group of chitosan scaffold.
29736686	4	44	theme	early	797:801	arg1	marker					803:808	an early marker	794:808	an early marker of osteogenic differentiation (ALP)	794:844	Both scaffolds bioactivated with organic and inorganic signals induce higher expression of an early marker of osteogenic differentiation (ALP) than the neat scaffolds after 3 days of cell culture.
29736686	1	45	theme	bioactive	189:197	arg1	scaffold					214:221	a bioactive chitosan-based scaffold	187:221	a bioactive chitosan-based scaffold functionalized with organic and inorganic signals	187:271	The present work is focused on the design of a bioactive chitosan-based scaffold functionalized with organic and inorganic signals to provide the biochemical cues for promoting stem cell osteogenic commitment.
29736686	0	46	theme	organic	24:30	arg1	decoration					50:59	inorganic and organic bioactive signals decoration	10:59	inorganic and organic bioactive signals decoration	10:59	Effect of inorganic and organic bioactive signals decoration on the biological performance of chitosan scaffolds for bone tissue engineering.
29736686	4	47	theme	culture	891:897	arg1	days					878:881	3 days	876:881	3 days of cell culture	876:897	Both scaffolds bioactivated with organic and inorganic signals induce higher expression of an early marker of osteogenic differentiation (ALP) than the neat scaffolds after 3 days of cell culture.
29736686	6	48	theme	hMSC	1168:1171	arg1	differentiation					1184:1198	hMSC osteogenic differentiation	1168:1198	hMSC osteogenic differentiation	1168:1198	Nevertheless, the biomineralized scaffolds showed better cellular behaviour than neat scaffolds, demonstrating the good effect of hydroxyapatite deposits on hMSC osteogenic differentiation.
29736686	3	49	theme	second	572:577	arg1	Meanwhile					557:565	Meanwhile	557:565	Meanwhile	557:565	Meanwhile, the second approach is based on the biomimetic treatment, which allows the formation of hydroxyapatite nuclei on the scaffold surface.
29736686	3	49	theme	second	572:577	arg1	approach					579:586	the second approach	568:586	the second approach	568:586	Meanwhile, the second approach is based on the biomimetic treatment, which allows the formation of hydroxyapatite nuclei on the scaffold surface.
29736686	9	50	from	presence	1478:1485	arg1	surface					1524:1530	the scaffold surface	1511:1530	the scaffold surface	1511:1530	The overall results showed that the presence of bioactive signals on the scaffold surface allows an osteoinductive effect on hMSC in a basal medium, making the modified chitosan scaffolds a promising candidate for bone tissue regeneration.
29736686	1	51	theme	chitosan-based	199:212	arg1	scaffold					214:221	a bioactive chitosan-based scaffold	187:221	a bioactive chitosan-based scaffold functionalized with organic and inorganic signals	187:271	The present work is focused on the design of a bioactive chitosan-based scaffold functionalized with organic and inorganic signals to provide the biochemical cues for promoting stem cell osteogenic commitment.
29736686	0	52	theme	decoration	50:59	arg1	Effect					0:5	Effect	0:5	Effect of inorganic and organic bioactive signals decoration on the biological performance of chitosan	0:101	Effect of inorganic and organic bioactive signals decoration on the biological performance of chitosan scaffolds for bone tissue engineering.
29736686	3	53	theme	biomimetic	604:613	arg1	treatment					615:623	the biomimetic treatment	600:623	the biomimetic treatment	600:623	Meanwhile, the second approach is based on the biomimetic treatment, which allows the formation of hydroxyapatite nuclei on the scaffold surface.
29736686	5	54	theme	biomineralized	991:1004	arg1	one					1006:1008	the biomineralized one	987:1008	the biomineralized one	987:1008	However, scaffolds decorated with BMP-mimicking peptide show higher values of ALP than the biomineralized one.
29736686	9	55	theme	bioactive	1490:1498	arg1	signals					1500:1506	bioactive signals	1490:1506	bioactive signals	1490:1506	The overall results showed that the presence of bioactive signals on the scaffold surface allows an osteoinductive effect on hMSC in a basal medium, making the modified chitosan scaffolds a promising candidate for bone tissue regeneration.
29736686	0	56	theme	signals	42:48	arg1	decoration					50:59	inorganic and organic bioactive signals decoration	10:59	inorganic and organic bioactive signals decoration	10:59	Effect of inorganic and organic bioactive signals decoration on the biological performance of chitosan scaffolds for bone tissue engineering.
29736686	1	57	theme	stem	319:322	arg1	commitment					340:349	stem cell osteogenic commitment	319:349	stem cell osteogenic commitment	319:349	The present work is focused on the design of a bioactive chitosan-based scaffold functionalized with organic and inorganic signals to provide the biochemical cues for promoting stem cell osteogenic commitment.
29736686	2	58	theme	scaffold	547:554	arg1	group					529:533	the carboxyl group	516:533	the carboxyl group of chitosan scaffold	516:554	The first approach is based on the use of a sequence of 20 amino acids corresponding to a 68-87 sequence in knuckle epitope of BMP-2 that was coupled covalently to the carboxyl group of chitosan scaffold.
29736686	2	58	theme	scaffold	547:554	arg1	scaffold					547:554	chitosan scaffold	538:554	chitosan scaffold	538:554	The first approach is based on the use of a sequence of 20 amino acids corresponding to a 68-87 sequence in knuckle epitope of BMP-2 that was coupled covalently to the carboxyl group of chitosan scaffold.
29736686	9	59	theme	osteoinductive	1542:1555	arg1	effect					1557:1562	an osteoinductive effect	1539:1562	an osteoinductive effect on hMSC	1539:1570	The overall results showed that the presence of bioactive signals on the scaffold surface allows an osteoinductive effect on hMSC in a basal medium, making the modified chitosan scaffolds a promising candidate for bone tissue regeneration.
29736686	4	60	theme	marker	803:808	arg1	expression					780:789	higher expression	773:789	higher expression of an early marker of osteogenic differentiation (ALP)	773:844	Both scaffolds bioactivated with organic and inorganic signals induce higher expression of an early marker of osteogenic differentiation (ALP) than the neat scaffolds after 3 days of cell culture.
29736686	9	61	theme	tissue	1661:1666	arg1	regeneration					1668:1679	bone tissue regeneration	1656:1679	bone tissue regeneration	1656:1679	The overall results showed that the presence of bioactive signals on the scaffold surface allows an osteoinductive effect on hMSC in a basal medium, making the modified chitosan scaffolds a promising candidate for bone tissue regeneration.
29736686	3	62	theme	nuclei	671:676	arg1	formation					643:651	the formation	639:651	the formation of hydroxyapatite nuclei on the scaffold surface	639:700	Meanwhile, the second approach is based on the biomimetic treatment, which allows the formation of hydroxyapatite nuclei on the scaffold surface.
29736686	1	63	theme	cell	324:327	arg1	commitment					340:349	stem cell osteogenic commitment	319:349	stem cell osteogenic commitment	319:349	The present work is focused on the design of a bioactive chitosan-based scaffold functionalized with organic and inorganic signals to provide the biochemical cues for promoting stem cell osteogenic commitment.
29736686	2	64	theme	chitosan	538:545	arg1	scaffold					547:554	chitosan scaffold	538:554	chitosan scaffold	538:554	The first approach is based on the use of a sequence of 20 amino acids corresponding to a 68-87 sequence in knuckle epitope of BMP-2 that was coupled covalently to the carboxyl group of chitosan scaffold.
29736686	5	65	theme	higher	961:966	arg1	values					968:973	higher values	961:973	higher values of ALP	961:980	However, scaffolds decorated with BMP-mimicking peptide show higher values of ALP than the biomineralized one.
29736686	4	66	theme	higher	773:778	arg1	expression					780:789	higher expression	773:789	higher expression of an early marker of osteogenic differentiation (ALP)	773:844	Both scaffolds bioactivated with organic and inorganic signals induce higher expression of an early marker of osteogenic differentiation (ALP) than the neat scaffolds after 3 days of cell culture.
29736686	9	67	theme	promising	1632:1640	arg1	candidate					1642:1650	a promising candidate	1630:1650	a promising candidate for bone tissue regeneration	1630:1679	The overall results showed that the presence of bioactive signals on the scaffold surface allows an osteoinductive effect on hMSC in a basal medium, making the modified chitosan scaffolds a promising candidate for bone tissue regeneration.
29736686	1	68	theme	scaffold	214:221	arg1	design					177:182	the design	173:182	the design of a bioactive chitosan-based scaffold functionalized with organic and inorganic signals to provide the biochemical cues for promoting stem cell osteogenic commitment	173:349	The present work is focused on the design of a bioactive chitosan-based scaffold functionalized with organic and inorganic signals to provide the biochemical cues for promoting stem cell osteogenic commitment.
29736686	6	69	theme	deposits	1156:1163	arg1	effect					1131:1136	the good effect	1122:1136	the good effect of hydroxyapatite deposits on hMSC osteogenic differentiation	1122:1198	Nevertheless, the biomineralized scaffolds showed better cellular behaviour than neat scaffolds, demonstrating the good effect of hydroxyapatite deposits on hMSC osteogenic differentiation.
29736686	8	70	theme	expression	1364:1373	arg1	level					1343:1347	the highest level	1331:1347	the highest level of Osteocalcin expression (OCN)	1331:1379	Furthermore, the highest level of Osteocalcin expression (OCN) was observed for scaffold with BMP2 mimic-peptide at day 21.
29736686	7	71	theme	biomineralized	1261:1274	arg1	scaffolds					1294:1302	the biomineralized and BMP-activated scaffolds	1257:1302	the biomineralized and BMP-activated scaffolds	1257:1302	At long incubation time no significant difference among the biomineralized and BMP-activated scaffolds was observed.
29736686	8	72	theme	Osteocalcin	1352:1362	arg1	OCN					1376:1378	OCN	1376:1378	OCN	1376:1378	Furthermore, the highest level of Osteocalcin expression (OCN) was observed for scaffold with BMP2 mimic-peptide at day 21.
29736686	8	72	theme	Osteocalcin	1352:1362	arg1	expression					1364:1373	Osteocalcin expression	1352:1373	Osteocalcin expression (OCN)	1352:1379	Furthermore, the highest level of Osteocalcin expression (OCN) was observed for scaffold with BMP2 mimic-peptide at day 21.
29736686	2	73	theme	BMP-2	479:483	arg1	epitope					468:474	knuckle epitope	460:474	knuckle epitope of BMP-2	460:483	The first approach is based on the use of a sequence of 20 amino acids corresponding to a 68-87 sequence in knuckle epitope of BMP-2 that was coupled covalently to the carboxyl group of chitosan scaffold.
29736686	4	74	theme	cell	886:889	arg1	culture					891:897	cell culture	886:897	cell culture	886:897	Both scaffolds bioactivated with organic and inorganic signals induce higher expression of an early marker of osteogenic differentiation (ALP) than the neat scaffolds after 3 days of cell culture.
29736686	9	75	theme	scaffold	1515:1522	arg1	surface					1524:1530	the scaffold surface	1511:1530	the scaffold surface	1511:1530	The overall results showed that the presence of bioactive signals on the scaffold surface allows an osteoinductive effect on hMSC in a basal medium, making the modified chitosan scaffolds a promising candidate for bone tissue regeneration.
29736686	6	76	theme	neat	1092:1095	arg1	scaffolds					1097:1105	neat scaffolds	1092:1105	neat scaffolds	1092:1105	Nevertheless, the biomineralized scaffolds showed better cellular behaviour than neat scaffolds, demonstrating the good effect of hydroxyapatite deposits on hMSC osteogenic differentiation.
29736686	9	77	theme	bone	1656:1659	arg1	regeneration					1668:1679	bone tissue regeneration	1656:1679	bone tissue regeneration	1656:1679	The overall results showed that the presence of bioactive signals on the scaffold surface allows an osteoinductive effect on hMSC in a basal medium, making the modified chitosan scaffolds a promising candidate for bone tissue regeneration.
29736686	7	78	theme	significant	1228:1238	arg1	difference					1240:1249	no significant difference	1225:1249	no significant difference among the biomineralized and BMP-activated scaffolds	1225:1302	At long incubation time no significant difference among the biomineralized and BMP-activated scaffolds was observed.
29736686	1	79	theme	osteogenic	329:338	arg1	commitment					340:349	stem cell osteogenic commitment	319:349	stem cell osteogenic commitment	319:349	The present work is focused on the design of a bioactive chitosan-based scaffold functionalized with organic and inorganic signals to provide the biochemical cues for promoting stem cell osteogenic commitment.
29736686	0	80	theme	inorganic	10:18	arg1	decoration					50:59	inorganic and organic bioactive signals decoration	10:59	inorganic and organic bioactive signals decoration	10:59	Effect of inorganic and organic bioactive signals decoration on the biological performance of chitosan scaffolds for bone tissue engineering.
29736686	1	81	theme	present	146:152	arg1	work					154:157	The present work	142:157	The present work	142:157	The present work is focused on the design of a bioactive chitosan-based scaffold functionalized with organic and inorganic signals to provide the biochemical cues for promoting stem cell osteogenic commitment.
29736686	9	82	theme	modified	1602:1609	arg1	chitosan					1611:1618	the modified chitosan	1598:1618	the modified chitosan	1598:1618	The overall results showed that the presence of bioactive signals on the scaffold surface allows an osteoinductive effect on hMSC in a basal medium, making the modified chitosan scaffolds a promising candidate for bone tissue regeneration.
29736686	1	83	theme	organic	243:249	arg1	signals					265:271	organic and inorganic signals	243:271	organic and inorganic signals	243:271	The present work is focused on the design of a bioactive chitosan-based scaffold functionalized with organic and inorganic signals to provide the biochemical cues for promoting stem cell osteogenic commitment.
29525638	0	0	theme	bone	101:104	arg1	cultures					146:153	human bone marrow-derived mesenchymal stromal cell cultures	95:153	human bone marrow-derived mesenchymal stromal cell cultures	95:153	In vitro osteogenic potential of collagen/chitosan-based hydrogels-silica particles hybrids in human bone marrow-derived mesenchymal stromal cell cultures.
29525638	3	1	from	activity	709:716	arg1	hBMSCs					721:726	hBMSCs	721:726	hBMSCs which was particularly pronounced for collagen/chitosan based hybrids	721:796	It was revealed that all tested materials are biocompatible and significantly enhance ALP activity in hBMSCs which was particularly pronounced for collagen/chitosan based hybrids.
29525638	0	2	link	marrow-derived	106:119	arg1	cultures					146:153	human bone marrow-derived mesenchymal stromal cell cultures	95:153	human bone marrow-derived mesenchymal stromal cell cultures	95:153	In vitro osteogenic potential of collagen/chitosan-based hydrogels-silica particles hybrids in human bone marrow-derived mesenchymal stromal cell cultures.
29525638	1	3	theme	study	172:176	arg1	aim					160:162	The aim	156:162	The aim of this study	156:176	The aim of this study was to assess osteogenic potential of three groups of biopolymeric hydrogel-based surfaces made of plain collagen, chitosan or collagen/chitosan, crosslinked with genipin or all three biopolymers modified with silica particles of two sizes (S1=240nm and S2=450nm).
29525638	5	4	theme	ColCh-based	1095:1105	arg1	hydrogels					1107:1115	ColCh-based hydrogels	1095:1115	ColCh-based hydrogels	1095:1115	SEM and EDS analyses of materials carried out after 20days of hBMSCs culturing on ColCh-based hydrogels revealed that the hybrid materials enhanced hBMSCs-mediated mineralization of ECM.
29525638	0	5	theme	human	95:99	arg1	cultures					146:153	human bone marrow-derived mesenchymal stromal cell cultures	95:153	human bone marrow-derived mesenchymal stromal cell cultures	95:153	In vitro osteogenic potential of collagen/chitosan-based hydrogels-silica particles hybrids in human bone marrow-derived mesenchymal stromal cell cultures.
29525638	4	6	theme	collagen/chitosan	890:906	arg1	materials					908:916	collagen/chitosan materials	890:916	collagen/chitosan materials	890:916	Gene expression (RUNX-2, COL-I, OC and VEGF mRNA) analyses performed in hBMSCs cultured at collagen/chitosan materials showed that ColChS1 hybrid the most effectively promotes osteogenic differentiation of hBMSCs.
29525638	1	7	theme	plain	277:281	arg1	collagen					283:290	plain collagen	277:290	plain collagen	277:290	The aim of this study was to assess osteogenic potential of three groups of biopolymeric hydrogel-based surfaces made of plain collagen, chitosan or collagen/chitosan, crosslinked with genipin or all three biopolymers modified with silica particles of two sizes (S1=240nm and S2=450nm).
29525638	0	8	theme	mesenchymal	121:131	arg1	cultures					146:153	human bone marrow-derived mesenchymal stromal cell cultures	95:153	human bone marrow-derived mesenchymal stromal cell cultures	95:153	In vitro osteogenic potential of collagen/chitosan-based hydrogels-silica particles hybrids in human bone marrow-derived mesenchymal stromal cell cultures.
29525638	7	9	theme	small	1596:1600	arg1	losses					1607:1612	small bone losses	1596:1612	small bone losses	1596:1612	That suggests that ColChS1 having the intrinsic osteoinductive activity holds great potential as material of choice for bone regeneration procedures, especially in regeneration of small bone losses.
29525638	5	10	theme	hBMSCs	1075:1080	arg1	culturing					1082:1090	hBMSCs culturing	1075:1090	hBMSCs culturing	1075:1090	SEM and EDS analyses of materials carried out after 20days of hBMSCs culturing on ColCh-based hydrogels revealed that the hybrid materials enhanced hBMSCs-mediated mineralization of ECM.
29525638	7	11	theme	regeneration	1541:1552	arg1	procedures					1554:1563	bone regeneration procedures	1536:1563	bone regeneration procedures	1536:1563	That suggests that ColChS1 having the intrinsic osteoinductive activity holds great potential as material of choice for bone regeneration procedures, especially in regeneration of small bone losses.
29525638	2	12	link	marrow-derived	550:563	arg1	hBMSCs					592:597	hBMSCs	592:597	hBMSCs	592:597	Biocompatibility and osteoinductive properties of the resulting composites were analyzed in the human bone marrow-derived mesenchymal stromal cells (hBMSCs) in vitro cultures.
29525638	2	12	link	marrow-derived	550:563	arg1	cells					585:589	the human bone marrow-derived mesenchymal stromal cells	535:589	the human bone marrow-derived mesenchymal stromal cells (hBMSCs) in vitro cultures	535:616	Biocompatibility and osteoinductive properties of the resulting composites were analyzed in the human bone marrow-derived mesenchymal stromal cells (hBMSCs) in vitro cultures.
29525638	0	13	theme	marrow-derived	106:119	arg1	cultures					146:153	human bone marrow-derived mesenchymal stromal cell cultures	95:153	human bone marrow-derived mesenchymal stromal cell cultures	95:153	In vitro osteogenic potential of collagen/chitosan-based hydrogels-silica particles hybrids in human bone marrow-derived mesenchymal stromal cell cultures.
29525638	3	14	theme	collagen/chitosan	766:782	arg1	hybrids					790:796	collagen/chitosan based hybrids	766:796	collagen/chitosan based hybrids	766:796	It was revealed that all tested materials are biocompatible and significantly enhance ALP activity in hBMSCs which was particularly pronounced for collagen/chitosan based hybrids.
29525638	6	15	theme	silica	1268:1273	arg1	particles					1275:1283	silica particles	1268:1283	silica particles of smaller sizes (ColChS1)	1268:1310	Our studies revealed that collagen/chitosan hydrogels modified with silica particles of smaller sizes (ColChS1) exhibit high pro-osteogenic properties without the need of applying any additional osteogenic inducers.
29525638	5	16	theme	EDS	1021:1023	arg1	analyses					1025:1032	SEM and EDS analyses	1013:1032	SEM and EDS analyses of materials carried out after 20days of hBMSCs culturing on ColCh-based hydrogels	1013:1115	SEM and EDS analyses of materials carried out after 20days of hBMSCs culturing on ColCh-based hydrogels revealed that the hybrid materials enhanced hBMSCs-mediated mineralization of ECM.
29525638	0	17	theme	cell	141:144	arg1	cultures					146:153	human bone marrow-derived mesenchymal stromal cell cultures	95:153	human bone marrow-derived mesenchymal stromal cell cultures	95:153	In vitro osteogenic potential of collagen/chitosan-based hydrogels-silica particles hybrids in human bone marrow-derived mesenchymal stromal cell cultures.
29525638	1	18	theme	silica	388:393	arg1	particles					395:403	silica particles	388:403	silica particles of two sizes (S1=240nm and S2=450nm)	388:440	The aim of this study was to assess osteogenic potential of three groups of biopolymeric hydrogel-based surfaces made of plain collagen, chitosan or collagen/chitosan, crosslinked with genipin or all three biopolymers modified with silica particles of two sizes (S1=240nm and S2=450nm).
29525638	7	19	theme	choice	1525:1530	arg1	material					1513:1520	material	1513:1520	material of choice	1513:1530	That suggests that ColChS1 having the intrinsic osteoinductive activity holds great potential as material of choice for bone regeneration procedures, especially in regeneration of small bone losses.
29525638	0	20	theme	stromal	133:139	arg1	cultures					146:153	human bone marrow-derived mesenchymal stromal cell cultures	95:153	human bone marrow-derived mesenchymal stromal cell cultures	95:153	In vitro osteogenic potential of collagen/chitosan-based hydrogels-silica particles hybrids in human bone marrow-derived mesenchymal stromal cell cultures.
29525638	2	21	theme	composites	507:516	arg1	properties					479:488	Biocompatibility and osteoinductive properties	443:488	Biocompatibility and osteoinductive properties of the resulting composites	443:516	Biocompatibility and osteoinductive properties of the resulting composites were analyzed in the human bone marrow-derived mesenchymal stromal cells (hBMSCs) in vitro cultures.
29525638	2	22	theme	osteoinductive	464:477	arg1	properties					479:488	Biocompatibility and osteoinductive properties	443:488	Biocompatibility and osteoinductive properties of the resulting composites	443:516	Biocompatibility and osteoinductive properties of the resulting composites were analyzed in the human bone marrow-derived mesenchymal stromal cells (hBMSCs) in vitro cultures.
29525638	5	23	theme	hBMSCs-mediated	1161:1175	arg1	mineralization					1177:1190	hBMSCs-mediated mineralization	1161:1190	hBMSCs-mediated mineralization of ECM	1161:1197	SEM and EDS analyses of materials carried out after 20days of hBMSCs culturing on ColCh-based hydrogels revealed that the hybrid materials enhanced hBMSCs-mediated mineralization of ECM.
29525638	2	24	theme	bone	545:548	arg1	hBMSCs					592:597	hBMSCs	592:597	hBMSCs	592:597	Biocompatibility and osteoinductive properties of the resulting composites were analyzed in the human bone marrow-derived mesenchymal stromal cells (hBMSCs) in vitro cultures.
29525638	2	24	theme	bone	545:548	arg1	cells					585:589	the human bone marrow-derived mesenchymal stromal cells	535:589	the human bone marrow-derived mesenchymal stromal cells (hBMSCs) in vitro cultures	535:616	Biocompatibility and osteoinductive properties of the resulting composites were analyzed in the human bone marrow-derived mesenchymal stromal cells (hBMSCs) in vitro cultures.
29525638	7	25	theme	bone	1602:1605	arg1	losses					1607:1612	small bone losses	1596:1612	small bone losses	1596:1612	That suggests that ColChS1 having the intrinsic osteoinductive activity holds great potential as material of choice for bone regeneration procedures, especially in regeneration of small bone losses.
29525638	4	26	theme	hBMSCs	1005:1010	arg1	differentiation					986:1000	osteogenic differentiation	975:1000	osteogenic differentiation of hBMSCs	975:1010	Gene expression (RUNX-2, COL-I, OC and VEGF mRNA) analyses performed in hBMSCs cultured at collagen/chitosan materials showed that ColChS1 hybrid the most effectively promotes osteogenic differentiation of hBMSCs.
29525638	3	27	theme	based	784:788	arg1	hybrids					790:796	collagen/chitosan based hybrids	766:796	collagen/chitosan based hybrids	766:796	It was revealed that all tested materials are biocompatible and significantly enhance ALP activity in hBMSCs which was particularly pronounced for collagen/chitosan based hybrids.
29525638	1	28	theme	osteogenic	192:201	arg1	potential					203:211	osteogenic potential	192:211	osteogenic potential of three groups of biopolymeric hydrogel-based surfaces made of plain collagen, chitosan or collagen/chitosan	192:321	The aim of this study was to assess osteogenic potential of three groups of biopolymeric hydrogel-based surfaces made of plain collagen, chitosan or collagen/chitosan, crosslinked with genipin or all three biopolymers modified with silica particles of two sizes (S1=240nm and S2=450nm).
29525638	0	29	theme	In	0:1	arg1	potential					20:28	In vitro osteogenic potential	0:28	In vitro osteogenic potential of collagen/chitosan-based hydrogels-silica particles	0:82	In vitro osteogenic potential of collagen/chitosan-based hydrogels-silica particles hybrids in human bone marrow-derived mesenchymal stromal cell cultures.
29525638	6	30	theme	pro-osteogenic	1325:1338	arg1	properties					1340:1349	high pro-osteogenic properties	1320:1349	high pro-osteogenic properties	1320:1349	Our studies revealed that collagen/chitosan hydrogels modified with silica particles of smaller sizes (ColChS1) exhibit high pro-osteogenic properties without the need of applying any additional osteogenic inducers.
29525638	7	31	theme	losses	1607:1612	arg1	regeneration					1580:1591	regeneration	1580:1591	regeneration of small bone losses	1580:1612	That suggests that ColChS1 having the intrinsic osteoinductive activity holds great potential as material of choice for bone regeneration procedures, especially in regeneration of small bone losses.
29525638	1	32	dep	was	178:180	arg1	crosslinked					324:334	crosslinked	324:334	crosslinked with genipin or all three biopolymers modified with silica particles of two sizes (S1=240nm and S2=450nm)	324:440	The aim of this study was to assess osteogenic potential of three groups of biopolymeric hydrogel-based surfaces made of plain collagen, chitosan or collagen/chitosan, crosslinked with genipin or all three biopolymers modified with silica particles of two sizes (S1=240nm and S2=450nm).
29525638	7	33	theme	osteoinductive	1464:1477	arg1	activity					1479:1486	the intrinsic osteoinductive activity	1450:1486	the intrinsic osteoinductive activity	1450:1486	That suggests that ColChS1 having the intrinsic osteoinductive activity holds great potential as material of choice for bone regeneration procedures, especially in regeneration of small bone losses.
29525638	7	34	contain	having	1443:1448	arg2	activity					1479:1486	the intrinsic osteoinductive activity	1450:1486	the intrinsic osteoinductive activity	1450:1486	That suggests that ColChS1 having the intrinsic osteoinductive activity holds great potential as material of choice for bone regeneration procedures, especially in regeneration of small bone losses.
29525638	7	34	contain	having	1443:1448	arg1	ColChS1					1435:1441	ColChS1	1435:1441	ColChS1 having the intrinsic osteoinductive activity	1435:1486	That suggests that ColChS1 having the intrinsic osteoinductive activity holds great potential as material of choice for bone regeneration procedures, especially in regeneration of small bone losses.
29525638	6	35	theme	smaller	1288:1294	arg1	sizes					1296:1300	smaller sizes	1288:1300	smaller sizes (ColChS1)	1288:1310	Our studies revealed that collagen/chitosan hydrogels modified with silica particles of smaller sizes (ColChS1) exhibit high pro-osteogenic properties without the need of applying any additional osteogenic inducers.
29525638	6	35	theme	smaller	1288:1294	arg1	ColChS1					1303:1309	ColChS1	1303:1309	ColChS1	1303:1309	Our studies revealed that collagen/chitosan hydrogels modified with silica particles of smaller sizes (ColChS1) exhibit high pro-osteogenic properties without the need of applying any additional osteogenic inducers.
29525638	4	36	theme	expression	804:813	arg1	analyses					849:856	Gene expression (RUNX-2, COL-I, OC and VEGF mRNA) analyses	799:856	Gene expression (RUNX-2, COL-I, OC and VEGF mRNA) analyses performed in hBMSCs cultured at collagen/chitosan materials	799:916	Gene expression (RUNX-2, COL-I, OC and VEGF mRNA) analyses performed in hBMSCs cultured at collagen/chitosan materials showed that ColChS1 hybrid the most effectively promotes osteogenic differentiation of hBMSCs.
29525638	5	37	theme	SEM	1013:1015	arg1	analyses					1025:1032	SEM and EDS analyses	1013:1032	SEM and EDS analyses of materials carried out after 20days of hBMSCs culturing on ColCh-based hydrogels	1013:1115	SEM and EDS analyses of materials carried out after 20days of hBMSCs culturing on ColCh-based hydrogels revealed that the hybrid materials enhanced hBMSCs-mediated mineralization of ECM.
29525638	0	38	theme	osteogenic	9:18	arg1	potential					20:28	In vitro osteogenic potential	0:28	In vitro osteogenic potential of collagen/chitosan-based hydrogels-silica particles	0:82	In vitro osteogenic potential of collagen/chitosan-based hydrogels-silica particles hybrids in human bone marrow-derived mesenchymal stromal cell cultures.
29525638	4	39	theme	osteogenic	975:984	arg1	differentiation					986:1000	osteogenic differentiation	975:1000	osteogenic differentiation of hBMSCs	975:1010	Gene expression (RUNX-2, COL-I, OC and VEGF mRNA) analyses performed in hBMSCs cultured at collagen/chitosan materials showed that ColChS1 hybrid the most effectively promotes osteogenic differentiation of hBMSCs.
29525638	4	40	theme	Gene	799:802	arg1	expression					804:813	Gene expression	799:813	Gene expression (RUNX-2, COL-I, OC and VEGF mRNA) analyses performed in hBMSCs cultured at collagen/chitosan materials	799:916	Gene expression (RUNX-2, COL-I, OC and VEGF mRNA) analyses performed in hBMSCs cultured at collagen/chitosan materials showed that ColChS1 hybrid the most effectively promotes osteogenic differentiation of hBMSCs.
29525638	6	41	theme	collagen/chitosan	1226:1242	arg1	hydrogels					1244:1252	collagen/chitosan hydrogels	1226:1252	collagen/chitosan hydrogels modified with silica particles of smaller sizes (ColChS1)	1226:1310	Our studies revealed that collagen/chitosan hydrogels modified with silica particles of smaller sizes (ColChS1) exhibit high pro-osteogenic properties without the need of applying any additional osteogenic inducers.
29525638	0	42	theme	collagen/chitosan-based	33:55	arg1	particles					74:82	collagen/chitosan-based hydrogels-silica particles	33:82	collagen/chitosan-based hydrogels-silica particles	33:82	In vitro osteogenic potential of collagen/chitosan-based hydrogels-silica particles hybrids in human bone marrow-derived mesenchymal stromal cell cultures.
29525638	1	43	theme	sizes	412:416	arg1	particles					395:403	silica particles	388:403	silica particles of two sizes (S1=240nm and S2=450nm)	388:440	The aim of this study was to assess osteogenic potential of three groups of biopolymeric hydrogel-based surfaces made of plain collagen, chitosan or collagen/chitosan, crosslinked with genipin or all three biopolymers modified with silica particles of two sizes (S1=240nm and S2=450nm).
29525638	6	44	theme	high	1320:1323	arg1	properties					1340:1349	high pro-osteogenic properties	1320:1349	high pro-osteogenic properties	1320:1349	Our studies revealed that collagen/chitosan hydrogels modified with silica particles of smaller sizes (ColChS1) exhibit high pro-osteogenic properties without the need of applying any additional osteogenic inducers.
29525638	1	45	theme	groups	222:227	arg1	potential					203:211	osteogenic potential	192:211	osteogenic potential of three groups of biopolymeric hydrogel-based surfaces made of plain collagen, chitosan or collagen/chitosan	192:321	The aim of this study was to assess osteogenic potential of three groups of biopolymeric hydrogel-based surfaces made of plain collagen, chitosan or collagen/chitosan, crosslinked with genipin or all three biopolymers modified with silica particles of two sizes (S1=240nm and S2=450nm).
29525638	2	46	theme	resulting	497:505	arg1	composites					507:516	the resulting composites	493:516	the resulting composites	493:516	Biocompatibility and osteoinductive properties of the resulting composites were analyzed in the human bone marrow-derived mesenchymal stromal cells (hBMSCs) in vitro cultures.
29525638	2	47	theme	stromal	577:583	arg1	hBMSCs					592:597	hBMSCs	592:597	hBMSCs	592:597	Biocompatibility and osteoinductive properties of the resulting composites were analyzed in the human bone marrow-derived mesenchymal stromal cells (hBMSCs) in vitro cultures.
29525638	2	47	theme	stromal	577:583	arg1	cells					585:589	the human bone marrow-derived mesenchymal stromal cells	535:589	the human bone marrow-derived mesenchymal stromal cells (hBMSCs) in vitro cultures	535:616	Biocompatibility and osteoinductive properties of the resulting composites were analyzed in the human bone marrow-derived mesenchymal stromal cells (hBMSCs) in vitro cultures.
29525638	5	48	theme	hybrid	1135:1140	arg1	materials					1142:1150	the hybrid materials	1131:1150	the hybrid materials	1131:1150	SEM and EDS analyses of materials carried out after 20days of hBMSCs culturing on ColCh-based hydrogels revealed that the hybrid materials enhanced hBMSCs-mediated mineralization of ECM.
29525638	2	49	theme	human	539:543	arg1	hBMSCs					592:597	hBMSCs	592:597	hBMSCs	592:597	Biocompatibility and osteoinductive properties of the resulting composites were analyzed in the human bone marrow-derived mesenchymal stromal cells (hBMSCs) in vitro cultures.
29525638	2	49	theme	human	539:543	arg1	cells					585:589	the human bone marrow-derived mesenchymal stromal cells	535:589	the human bone marrow-derived mesenchymal stromal cells (hBMSCs) in vitro cultures	535:616	Biocompatibility and osteoinductive properties of the resulting composites were analyzed in the human bone marrow-derived mesenchymal stromal cells (hBMSCs) in vitro cultures.
29525638	0	50	theme	particles	74:82	arg1	potential					20:28	In vitro osteogenic potential	0:28	In vitro osteogenic potential of collagen/chitosan-based hydrogels-silica particles	0:82	In vitro osteogenic potential of collagen/chitosan-based hydrogels-silica particles hybrids in human bone marrow-derived mesenchymal stromal cell cultures.
29525638	2	51	theme	mesenchymal	565:575	arg1	hBMSCs					592:597	hBMSCs	592:597	hBMSCs	592:597	Biocompatibility and osteoinductive properties of the resulting composites were analyzed in the human bone marrow-derived mesenchymal stromal cells (hBMSCs) in vitro cultures.
29525638	2	51	theme	mesenchymal	565:575	arg1	cells					585:589	the human bone marrow-derived mesenchymal stromal cells	535:589	the human bone marrow-derived mesenchymal stromal cells (hBMSCs) in vitro cultures	535:616	Biocompatibility and osteoinductive properties of the resulting composites were analyzed in the human bone marrow-derived mesenchymal stromal cells (hBMSCs) in vitro cultures.
29525638	7	52	theme	intrinsic	1454:1462	arg1	activity					1479:1486	the intrinsic osteoinductive activity	1450:1486	the intrinsic osteoinductive activity	1450:1486	That suggests that ColChS1 having the intrinsic osteoinductive activity holds great potential as material of choice for bone regeneration procedures, especially in regeneration of small bone losses.
29525638	0	53	from	hybrids	84:90	arg1	cultures					146:153	human bone marrow-derived mesenchymal stromal cell cultures	95:153	human bone marrow-derived mesenchymal stromal cell cultures	95:153	In vitro osteogenic potential of collagen/chitosan-based hydrogels-silica particles hybrids in human bone marrow-derived mesenchymal stromal cell cultures.
29525638	2	54	theme	marrow-derived	550:563	arg1	hBMSCs					592:597	hBMSCs	592:597	hBMSCs	592:597	Biocompatibility and osteoinductive properties of the resulting composites were analyzed in the human bone marrow-derived mesenchymal stromal cells (hBMSCs) in vitro cultures.
29525638	2	54	theme	marrow-derived	550:563	arg1	cells					585:589	the human bone marrow-derived mesenchymal stromal cells	535:589	the human bone marrow-derived mesenchymal stromal cells (hBMSCs) in vitro cultures	535:616	Biocompatibility and osteoinductive properties of the resulting composites were analyzed in the human bone marrow-derived mesenchymal stromal cells (hBMSCs) in vitro cultures.
29525638	4	55	dep	expression	804:813	arg1	RUNX-2					816:821	RUNX-2	816:821	RUNX-2	816:821	Gene expression (RUNX-2, COL-I, OC and VEGF mRNA) analyses performed in hBMSCs cultured at collagen/chitosan materials showed that ColChS1 hybrid the most effectively promotes osteogenic differentiation of hBMSCs.
29525638	4	55	dep	expression	804:813	arg1	OC					831:832	OC	831:832	OC	831:832	Gene expression (RUNX-2, COL-I, OC and VEGF mRNA) analyses performed in hBMSCs cultured at collagen/chitosan materials showed that ColChS1 hybrid the most effectively promotes osteogenic differentiation of hBMSCs.
29525638	4	55	dep	expression	804:813	arg1	mRNA					843:846	VEGF mRNA	838:846	VEGF mRNA	838:846	Gene expression (RUNX-2, COL-I, OC and VEGF mRNA) analyses performed in hBMSCs cultured at collagen/chitosan materials showed that ColChS1 hybrid the most effectively promotes osteogenic differentiation of hBMSCs.
29525638	4	55	dep	expression	804:813	arg1	COL-I					824:828	COL-I	824:828	COL-I	824:828	Gene expression (RUNX-2, COL-I, OC and VEGF mRNA) analyses performed in hBMSCs cultured at collagen/chitosan materials showed that ColChS1 hybrid the most effectively promotes osteogenic differentiation of hBMSCs.
29525638	0	56	theme	hydrogels-silica	57:72	arg1	particles					74:82	collagen/chitosan-based hydrogels-silica particles	33:82	collagen/chitosan-based hydrogels-silica particles	33:82	In vitro osteogenic potential of collagen/chitosan-based hydrogels-silica particles hybrids in human bone marrow-derived mesenchymal stromal cell cultures.
29525638	2	57	theme	vitro	603:607	arg1	cultures					609:616	vitro cultures	603:616	vitro cultures	603:616	Biocompatibility and osteoinductive properties of the resulting composites were analyzed in the human bone marrow-derived mesenchymal stromal cells (hBMSCs) in vitro cultures.
29525638	5	58	theme	culturing	1082:1090	arg1	20days					1065:1070	20days	1065:1070	20days of hBMSCs culturing	1065:1090	SEM and EDS analyses of materials carried out after 20days of hBMSCs culturing on ColCh-based hydrogels revealed that the hybrid materials enhanced hBMSCs-mediated mineralization of ECM.
29525638	7	59	theme	bone	1536:1539	arg1	procedures					1554:1563	bone regeneration procedures	1536:1563	bone regeneration procedures	1536:1563	That suggests that ColChS1 having the intrinsic osteoinductive activity holds great potential as material of choice for bone regeneration procedures, especially in regeneration of small bone losses.
29525638	6	60	theme	osteogenic	1395:1404	arg1	inducers					1406:1413	any additional osteogenic inducers	1380:1413	any additional osteogenic inducers	1380:1413	Our studies revealed that collagen/chitosan hydrogels modified with silica particles of smaller sizes (ColChS1) exhibit high pro-osteogenic properties without the need of applying any additional osteogenic inducers.
29525638	5	61	theme	materials	1037:1045	arg1	analyses					1025:1032	SEM and EDS analyses	1013:1032	SEM and EDS analyses of materials carried out after 20days of hBMSCs culturing on ColCh-based hydrogels	1013:1115	SEM and EDS analyses of materials carried out after 20days of hBMSCs culturing on ColCh-based hydrogels revealed that the hybrid materials enhanced hBMSCs-mediated mineralization of ECM.
29525638	3	62	theme	tested	644:649	arg1	materials					651:659	all tested materials	640:659	all tested materials	640:659	It was revealed that all tested materials are biocompatible and significantly enhance ALP activity in hBMSCs which was particularly pronounced for collagen/chitosan based hybrids.
29525638	1	63	theme	biopolymeric	232:243	arg1	surfaces					260:267	biopolymeric hydrogel-based surfaces	232:267	biopolymeric hydrogel-based surfaces made of plain collagen, chitosan or collagen/chitosan	232:321	The aim of this study was to assess osteogenic potential of three groups of biopolymeric hydrogel-based surfaces made of plain collagen, chitosan or collagen/chitosan, crosslinked with genipin or all three biopolymers modified with silica particles of two sizes (S1=240nm and S2=450nm).
29525638	6	64	theme	additional	1384:1393	arg1	inducers					1406:1413	any additional osteogenic inducers	1380:1413	any additional osteogenic inducers	1380:1413	Our studies revealed that collagen/chitosan hydrogels modified with silica particles of smaller sizes (ColChS1) exhibit high pro-osteogenic properties without the need of applying any additional osteogenic inducers.
29525638	6	65	theme	sizes	1296:1300	arg1	particles					1275:1283	silica particles	1268:1283	silica particles of smaller sizes (ColChS1)	1268:1310	Our studies revealed that collagen/chitosan hydrogels modified with silica particles of smaller sizes (ColChS1) exhibit high pro-osteogenic properties without the need of applying any additional osteogenic inducers.
29525638	7	66	theme	great	1494:1498	arg1	potential					1500:1508	great potential	1494:1508	great potential as material of choice for bone regeneration procedures	1494:1563	That suggests that ColChS1 having the intrinsic osteoinductive activity holds great potential as material of choice for bone regeneration procedures, especially in regeneration of small bone losses.
29525638	1	67	theme	hydrogel-based	245:258	arg1	surfaces					260:267	biopolymeric hydrogel-based surfaces	232:267	biopolymeric hydrogel-based surfaces made of plain collagen, chitosan or collagen/chitosan	232:321	The aim of this study was to assess osteogenic potential of three groups of biopolymeric hydrogel-based surfaces made of plain collagen, chitosan or collagen/chitosan, crosslinked with genipin or all three biopolymers modified with silica particles of two sizes (S1=240nm and S2=450nm).
29525638	3	68	theme	ALP	705:707	arg1	activity					709:716	ALP activity	705:716	ALP activity in hBMSCs which was particularly pronounced for collagen/chitosan based hybrids	705:796	It was revealed that all tested materials are biocompatible and significantly enhance ALP activity in hBMSCs which was particularly pronounced for collagen/chitosan based hybrids.
29525638	4	69	theme	VEGF	838:841	arg1	mRNA					843:846	VEGF mRNA	838:846	VEGF mRNA	838:846	Gene expression (RUNX-2, COL-I, OC and VEGF mRNA) analyses performed in hBMSCs cultured at collagen/chitosan materials showed that ColChS1 hybrid the most effectively promotes osteogenic differentiation of hBMSCs.
29525638	2	70	theme	Biocompatibility	443:458	arg1	properties					479:488	Biocompatibility and osteoinductive properties	443:488	Biocompatibility and osteoinductive properties of the resulting composites	443:516	Biocompatibility and osteoinductive properties of the resulting composites were analyzed in the human bone marrow-derived mesenchymal stromal cells (hBMSCs) in vitro cultures.
29525638	4	71	theme	ColChS1	930:936	arg1	hybrid					938:943	ColChS1 hybrid	930:943	ColChS1 hybrid	930:943	Gene expression (RUNX-2, COL-I, OC and VEGF mRNA) analyses performed in hBMSCs cultured at collagen/chitosan materials showed that ColChS1 hybrid the most effectively promotes osteogenic differentiation of hBMSCs.
29525638	1	72	theme	surfaces	260:267	arg1	surfaces					260:267	biopolymeric hydrogel-based surfaces	232:267	biopolymeric hydrogel-based surfaces made of plain collagen, chitosan or collagen/chitosan	232:321	The aim of this study was to assess osteogenic potential of three groups of biopolymeric hydrogel-based surfaces made of plain collagen, chitosan or collagen/chitosan, crosslinked with genipin or all three biopolymers modified with silica particles of two sizes (S1=240nm and S2=450nm).
29525638	1	72	theme	surfaces	260:267	arg1	groups					222:227	three groups	216:227	three groups of biopolymeric hydrogel-based surfaces made of plain collagen, chitosan or collagen/chitosan	216:321	The aim of this study was to assess osteogenic potential of three groups of biopolymeric hydrogel-based surfaces made of plain collagen, chitosan or collagen/chitosan, crosslinked with genipin or all three biopolymers modified with silica particles of two sizes (S1=240nm and S2=450nm).
29525638	5	73	theme	ECM	1195:1197	arg1	mineralization					1177:1190	hBMSCs-mediated mineralization	1161:1190	hBMSCs-mediated mineralization of ECM	1161:1197	SEM and EDS analyses of materials carried out after 20days of hBMSCs culturing on ColCh-based hydrogels revealed that the hybrid materials enhanced hBMSCs-mediated mineralization of ECM.
29525638	0	74	dep	In	0:1	arg1	vitro					3:7	vitro	3:7	vitro	3:7	In vitro osteogenic potential of collagen/chitosan-based hydrogels-silica particles hybrids in human bone marrow-derived mesenchymal stromal cell cultures.
29525638	2	75	from	cells	585:589	arg1	cultures					609:616	vitro cultures	603:616	vitro cultures	603:616	Biocompatibility and osteoinductive properties of the resulting composites were analyzed in the human bone marrow-derived mesenchymal stromal cells (hBMSCs) in vitro cultures.
29525638	1	76	dep	sizes	412:416	arg1	S2=450nm					432:439	S2=450nm	432:439	S2=450nm	432:439	The aim of this study was to assess osteogenic potential of three groups of biopolymeric hydrogel-based surfaces made of plain collagen, chitosan or collagen/chitosan, crosslinked with genipin or all three biopolymers modified with silica particles of two sizes (S1=240nm and S2=450nm).
29525638	1	76	dep	sizes	412:416	arg1	S1=240nm					419:426	S1=240nm	419:426	S1=240nm	419:426	The aim of this study was to assess osteogenic potential of three groups of biopolymeric hydrogel-based surfaces made of plain collagen, chitosan or collagen/chitosan, crosslinked with genipin or all three biopolymers modified with silica particles of two sizes (S1=240nm and S2=450nm).
29525638	1	76	dep	sizes	412:416	arg1	sizes					412:416	two sizes	408:416	two sizes (S1=240nm and S2=450nm)	408:440	The aim of this study was to assess osteogenic potential of three groups of biopolymeric hydrogel-based surfaces made of plain collagen, chitosan or collagen/chitosan, crosslinked with genipin or all three biopolymers modified with silica particles of two sizes (S1=240nm and S2=450nm).
31334694	14	0	theme	sequence	1434:1441	arg1	pattern					1544:1550	its unique lipid pattern	1527:1550	its unique lipid pattern	1527:1550	Based on the low 16S rRNA gene sequence similarities, its taxonomic position in the phylogenetic and phylogenomic trees and its unique lipid pattern, we propose that strains 313T and 352 represent members of a novel species in a new genus, for which the name Fudania jinshanensis gen. nov., sp.
31334694	14	0	theme	sequence	1434:1441	arg1	similarities					1443:1454	the low 16S rRNA gene sequence similarities	1412:1454	the low 16S rRNA gene sequence similarities	1412:1454	Based on the low 16S rRNA gene sequence similarities, its taxonomic position in the phylogenetic and phylogenomic trees and its unique lipid pattern, we propose that strains 313T and 352 represent members of a novel species in a new genus, for which the name Fudania jinshanensis gen. nov., sp.
31334694	14	0	theme	sequence	1434:1441	arg1	position					1471:1478	its taxonomic position	1457:1478	its taxonomic position in the phylogenetic and phylogenomic trees	1457:1521	Based on the low 16S rRNA gene sequence similarities, its taxonomic position in the phylogenetic and phylogenomic trees and its unique lipid pattern, we propose that strains 313T and 352 represent members of a novel species in a new genus, for which the name Fudania jinshanensis gen. nov., sp.
31334694	6	1	theme	Phylogenetic	616:627	arg1	member					811:816	a member	809:816	a member of any of these genera	809:839	Phylogenetic analyses showed that strains 313T and 352 clustered independently in the vicinity of the genera Actinotignum, Actinobaculum and Flaviflexus, but could not be classified clearly as a member of any of these genera.
31334694	6	1	theme	Phylogenetic	616:627	arg1	analyses					629:636	Phylogenetic analyses	616:636	Phylogenetic analyses	616:636	Phylogenetic analyses showed that strains 313T and 352 clustered independently in the vicinity of the genera Actinotignum, Actinobaculum and Flaviflexus, but could not be classified clearly as a member of any of these genera.
31334694	13	2	theme	DNA	1357:1359	arg1	G+C content					1361:1371	The DNA G+C content	1353:1371	The DNA G+C content of strain 313T	1353:1386	The DNA G+C content of strain 313T was 60.6 mol%.
31334694	13	2	theme	DNA	1357:1359	arg1	%					1400:1400	60.6 mol%	1392:1400	60.6 mol%	1392:1400	The DNA G+C content of strain 313T was 60.6 mol%.
31334694	1	3	from	faeces	56:61	arg1	China					113:117	China	113:117	China	113:117	nov., isolated from faeces of the Tibetan antelope (Pantholops hodgsonii) in China.
31334694	14	4	theme	new	1632:1634	arg1	genus					1636:1640	a new genus	1630:1640	a new genus	1630:1640	Based on the low 16S rRNA gene sequence similarities, its taxonomic position in the phylogenetic and phylogenomic trees and its unique lipid pattern, we propose that strains 313T and 352 represent members of a novel species in a new genus, for which the name Fudania jinshanensis gen. nov., sp.
31334694	6	5	theme	genera	718:723	arg1	vicinity					702:709	the vicinity	698:709	the vicinity of the genera Actinotignum, Actinobaculum and Flaviflexus	698:767	Phylogenetic analyses showed that strains 313T and 352 clustered independently in the vicinity of the genera Actinotignum, Actinobaculum and Flaviflexus, but could not be classified clearly as a member of any of these genera.
31334694	7	6	from	branch	927:932	arg1	Actinomycetaceae					948:963	the family Actinomycetaceae	937:963	the family Actinomycetaceae	937:963	Phylogenomic analysis also indicated that strains 313T and 352 formed an independent branch in the family Actinomycetaceae.
31334694	2	7	from	China	258:262	arg1	faeces					197:202	the faeces	193:202	the faeces of Tibetan antelopes on the Tibet-Qinghai Plateau, PR China	193:262	Two hitherto unknown bacteria (strains 313T and 352) were recovered from the faeces of Tibetan antelopes on the Tibet-Qinghai Plateau, PR China.
31334694	10	8	contain	contained	1219:1227	arg1	peptidoglycan					1205:1217	The peptidoglycan	1201:1217	The peptidoglycan	1201:1217	The peptidoglycan contained lysine, alanine and glutamic acid.
31334694	10	8	contain	contained	1219:1227	arg2	alanine					1237:1243	alanine	1237:1243	alanine	1237:1243	The peptidoglycan contained lysine, alanine and glutamic acid.
31334694	10	8	contain	contained	1219:1227	arg2	lysine					1229:1234	lysine	1229:1234	lysine	1229:1234	The peptidoglycan contained lysine, alanine and glutamic acid.
31334694	10	8	contain	contained	1219:1227	arg2	acid					1258:1261	glutamic acid	1249:1261	glutamic acid	1249:1261	The peptidoglycan contained lysine, alanine and glutamic acid.
31334694	9	9	theme	polar	1045:1049	arg1	lipids					1051:1056	The polar lipids	1041:1056	The polar lipids	1041:1056	The polar lipids comprised diphosphatidylglycerol, phosphatidylinositol mannoside, phosphatidylglycerol, phosphatidylinositol and five unidentified components.
31334694	2	10	theme	Tibet-Qinghai	232:244	arg1	China					258:262	the Tibet-Qinghai Plateau, PR China	228:262	China	258:262	Two hitherto unknown bacteria (strains 313T and 352) were recovered from the faeces of Tibetan antelopes on the Tibet-Qinghai Plateau, PR China.
31334694	2	11	theme	Plateau	246:252	arg1	China					258:262	the Tibet-Qinghai Plateau, PR China	228:262	China	258:262	Two hitherto unknown bacteria (strains 313T and 352) were recovered from the faeces of Tibetan antelopes on the Tibet-Qinghai Plateau, PR China.
31334694	14	12	theme	taxonomic	1461:1469	arg1	similarities					1443:1454	the low 16S rRNA gene sequence similarities	1412:1454	the low 16S rRNA gene sequence similarities	1412:1454	Based on the low 16S rRNA gene sequence similarities, its taxonomic position in the phylogenetic and phylogenomic trees and its unique lipid pattern, we propose that strains 313T and 352 represent members of a novel species in a new genus, for which the name Fudania jinshanensis gen. nov., sp.
31334694	14	12	theme	taxonomic	1461:1469	arg1	position					1471:1478	its taxonomic position	1457:1478	its taxonomic position in the phylogenetic and phylogenomic trees	1457:1521	Based on the low 16S rRNA gene sequence similarities, its taxonomic position in the phylogenetic and phylogenomic trees and its unique lipid pattern, we propose that strains 313T and 352 represent members of a novel species in a new genus, for which the name Fudania jinshanensis gen. nov., sp.
31334694	7	13	dep	strains	884:890	arg1	352					901:903	352	901:903	352	901:903	Phylogenomic analysis also indicated that strains 313T and 352 formed an independent branch in the family Actinomycetaceae.
31334694	7	13	dep	strains	884:890	arg1	313T					892:895	313T	892:895	313T	892:895	Phylogenomic analysis also indicated that strains 313T and 352 formed an independent branch in the family Actinomycetaceae.
31334694	5	14	theme	%	432:432	arg1	similarity					457:466	92.6 % 16S rRNA gene sequence similarity	427:466	92.6 % 16S rRNA gene sequence similarity	427:466	The isolates were closely related to Actinotignum sanguinis (92.6 % 16S rRNA gene sequence similarity), Arcanobacterium haemolyticum (92.5 %), Actinotignum schaalii (92.4 %), Actinobaculum massiliense (92.2 %) and Flaviflexus huanghaiensis (91.6 %).
31334694	5	14	theme	%	432:432	arg1	sanguinis					416:424	Actinotignum sanguinis	403:424	Actinotignum sanguinis (92.6 % 16S rRNA gene sequence similarity)	403:467	The isolates were closely related to Actinotignum sanguinis (92.6 % 16S rRNA gene sequence similarity), Arcanobacterium haemolyticum (92.5 %), Actinotignum schaalii (92.4 %), Actinobaculum massiliense (92.2 %) and Flaviflexus huanghaiensis (91.6 %).
31334694	14	15	theme	species	1619:1625	arg1	members					1600:1606	members	1600:1606	members of a novel species	1600:1625	Based on the low 16S rRNA gene sequence similarities, its taxonomic position in the phylogenetic and phylogenomic trees and its unique lipid pattern, we propose that strains 313T and 352 represent members of a novel species in a new genus, for which the name Fudania jinshanensis gen. nov., sp.
31334694	1	16	from	China	113:117	arg1	faeces					56:61	faeces	56:61	faeces of the Tibetan antelope (Pantholops hodgsonii) in China	56:117	nov., isolated from faeces of the Tibetan antelope (Pantholops hodgsonii) in China.
31334694	14	17	from	position	1471:1478	arg1	trees					1517:1521	the phylogenetic and phylogenomic trees	1483:1521	trees	1517:1521	Based on the low 16S rRNA gene sequence similarities, its taxonomic position in the phylogenetic and phylogenomic trees and its unique lipid pattern, we propose that strains 313T and 352 represent members of a novel species in a new genus, for which the name Fudania jinshanensis gen. nov., sp.
31334694	5	18	theme	16S	434:436	arg1	similarity					457:466	92.6 % 16S rRNA gene sequence similarity	427:466	92.6 % 16S rRNA gene sequence similarity	427:466	The isolates were closely related to Actinotignum sanguinis (92.6 % 16S rRNA gene sequence similarity), Arcanobacterium haemolyticum (92.5 %), Actinotignum schaalii (92.4 %), Actinobaculum massiliense (92.2 %) and Flaviflexus huanghaiensis (91.6 %).
31334694	5	18	theme	16S	434:436	arg1	sanguinis					416:424	Actinotignum sanguinis	403:424	Actinotignum sanguinis (92.6 % 16S rRNA gene sequence similarity)	403:467	The isolates were closely related to Actinotignum sanguinis (92.6 % 16S rRNA gene sequence similarity), Arcanobacterium haemolyticum (92.5 %), Actinotignum schaalii (92.4 %), Actinobaculum massiliense (92.2 %) and Flaviflexus huanghaiensis (91.6 %).
31334694	14	19	theme	low	1416:1418	arg1	pattern					1544:1550	its unique lipid pattern	1527:1550	its unique lipid pattern	1527:1550	Based on the low 16S rRNA gene sequence similarities, its taxonomic position in the phylogenetic and phylogenomic trees and its unique lipid pattern, we propose that strains 313T and 352 represent members of a novel species in a new genus, for which the name Fudania jinshanensis gen. nov., sp.
31334694	14	19	theme	low	1416:1418	arg1	similarities					1443:1454	the low 16S rRNA gene sequence similarities	1412:1454	the low 16S rRNA gene sequence similarities	1412:1454	Based on the low 16S rRNA gene sequence similarities, its taxonomic position in the phylogenetic and phylogenomic trees and its unique lipid pattern, we propose that strains 313T and 352 represent members of a novel species in a new genus, for which the name Fudania jinshanensis gen. nov., sp.
31334694	14	19	theme	low	1416:1418	arg1	position					1471:1478	its taxonomic position	1457:1478	its taxonomic position in the phylogenetic and phylogenomic trees	1457:1521	Based on the low 16S rRNA gene sequence similarities, its taxonomic position in the phylogenetic and phylogenomic trees and its unique lipid pattern, we propose that strains 313T and 352 represent members of a novel species in a new genus, for which the name Fudania jinshanensis gen. nov., sp.
31334694	14	20	theme	lipid	1538:1542	arg1	pattern					1544:1550	its unique lipid pattern	1527:1550	its unique lipid pattern	1527:1550	Based on the low 16S rRNA gene sequence similarities, its taxonomic position in the phylogenetic and phylogenomic trees and its unique lipid pattern, we propose that strains 313T and 352 represent members of a novel species in a new genus, for which the name Fudania jinshanensis gen. nov., sp.
31334694	14	20	theme	lipid	1538:1542	arg1	similarities					1443:1454	the low 16S rRNA gene sequence similarities	1412:1454	the low 16S rRNA gene sequence similarities	1412:1454	Based on the low 16S rRNA gene sequence similarities, its taxonomic position in the phylogenetic and phylogenomic trees and its unique lipid pattern, we propose that strains 313T and 352 represent members of a novel species in a new genus, for which the name Fudania jinshanensis gen. nov., sp.
31334694	4	21	theme	growth	324:329	arg1	conditions					331:340	The optimal growth conditions	312:340	The optimal growth conditions	312:340	The optimal growth conditions were at 37 °C and pH 7.
31334694	2	22	theme	antelopes	215:223	arg1	faeces					197:202	the faeces	193:202	the faeces of Tibetan antelopes on the Tibet-Qinghai Plateau, PR China	193:262	Two hitherto unknown bacteria (strains 313T and 352) were recovered from the faeces of Tibetan antelopes on the Tibet-Qinghai Plateau, PR China.
31334694	5	23	theme	rRNA	438:441	arg1	similarity					457:466	92.6 % 16S rRNA gene sequence similarity	427:466	92.6 % 16S rRNA gene sequence similarity	427:466	The isolates were closely related to Actinotignum sanguinis (92.6 % 16S rRNA gene sequence similarity), Arcanobacterium haemolyticum (92.5 %), Actinotignum schaalii (92.4 %), Actinobaculum massiliense (92.2 %) and Flaviflexus huanghaiensis (91.6 %).
31334694	5	23	theme	rRNA	438:441	arg1	sanguinis					416:424	Actinotignum sanguinis	403:424	Actinotignum sanguinis (92.6 % 16S rRNA gene sequence similarity)	403:467	The isolates were closely related to Actinotignum sanguinis (92.6 % 16S rRNA gene sequence similarity), Arcanobacterium haemolyticum (92.5 %), Actinotignum schaalii (92.4 %), Actinobaculum massiliense (92.2 %) and Flaviflexus huanghaiensis (91.6 %).
31334694	14	24	theme	name	1657:1660	arg1	nov.					1688:1691	the name Fudania jinshanensis gen. nov.	1653:1691	the name Fudania jinshanensis gen. nov.	1653:1691	Based on the low 16S rRNA gene sequence similarities, its taxonomic position in the phylogenetic and phylogenomic trees and its unique lipid pattern, we propose that strains 313T and 352 represent members of a novel species in a new genus, for which the name Fudania jinshanensis gen. nov., sp.
31334694	0	25	theme	jinshanensis	8:19	arg1	nov.					26:29	Fudania jinshanensis gen. nov.	0:29	Fudania jinshanensis gen. nov.	0:29	Fudania jinshanensis gen. nov., sp.
31334694	14	26	theme	gen.	1683:1686	arg1	nov.					1688:1691	the name Fudania jinshanensis gen. nov.	1653:1691	the name Fudania jinshanensis gen. nov.	1653:1691	Based on the low 16S rRNA gene sequence similarities, its taxonomic position in the phylogenetic and phylogenomic trees and its unique lipid pattern, we propose that strains 313T and 352 represent members of a novel species in a new genus, for which the name Fudania jinshanensis gen. nov., sp.
31334694	5	27	theme	Actinobaculum	541:553	arg1	massiliense					555:565	Actinobaculum massiliense	541:565	Actinobaculum massiliense (92.2 %)	541:574	The isolates were closely related to Actinotignum sanguinis (92.6 % 16S rRNA gene sequence similarity), Arcanobacterium haemolyticum (92.5 %), Actinotignum schaalii (92.4 %), Actinobaculum massiliense (92.2 %) and Flaviflexus huanghaiensis (91.6 %).
31334694	5	27	theme	Actinobaculum	541:553	arg1	%					573:573	92.2 %	568:573	92.2 %	568:573	The isolates were closely related to Actinotignum sanguinis (92.6 % 16S rRNA gene sequence similarity), Arcanobacterium haemolyticum (92.5 %), Actinotignum schaalii (92.4 %), Actinobaculum massiliense (92.2 %) and Flaviflexus huanghaiensis (91.6 %).
31334694	4	28	theme	optimal	316:322	arg1	conditions					331:340	The optimal growth conditions	312:340	The optimal growth conditions	312:340	The optimal growth conditions were at 37 °C and pH 7.
31334694	5	29	theme	gene	443:446	arg1	similarity					457:466	92.6 % 16S rRNA gene sequence similarity	427:466	92.6 % 16S rRNA gene sequence similarity	427:466	The isolates were closely related to Actinotignum sanguinis (92.6 % 16S rRNA gene sequence similarity), Arcanobacterium haemolyticum (92.5 %), Actinotignum schaalii (92.4 %), Actinobaculum massiliense (92.2 %) and Flaviflexus huanghaiensis (91.6 %).
31334694	5	29	theme	gene	443:446	arg1	sanguinis					416:424	Actinotignum sanguinis	403:424	Actinotignum sanguinis (92.6 % 16S rRNA gene sequence similarity)	403:467	The isolates were closely related to Actinotignum sanguinis (92.6 % 16S rRNA gene sequence similarity), Arcanobacterium haemolyticum (92.5 %), Actinotignum schaalii (92.4 %), Actinobaculum massiliense (92.2 %) and Flaviflexus huanghaiensis (91.6 %).
31334694	16	30	theme	=CGMCC	1741:1746	arg1	106216T					1760:1766	=CGMCC 4.7453T=DSM 106216T	1741:1766	=CGMCC 4.7453T=DSM 106216T	1741:1766	The type strain is 313T (=CGMCC 4.7453T=DSM 106216T).
31334694	16	30	theme	=CGMCC	1741:1746	arg1	313T					1735:1738	313T	1735:1738	313T (=CGMCC 4.7453T=DSM 106216T)	1735:1767	The type strain is 313T (=CGMCC 4.7453T=DSM 106216T).
31334694	0	31	theme	Fudania	0:6	arg1	nov.					26:29	Fudania jinshanensis gen. nov.	0:29	Fudania jinshanensis gen. nov.	0:29	Fudania jinshanensis gen. nov., sp.
31334694	14	32	from	pattern	1544:1550	arg1	trees					1517:1521	the phylogenetic and phylogenomic trees	1483:1521	trees	1517:1521	Based on the low 16S rRNA gene sequence similarities, its taxonomic position in the phylogenetic and phylogenomic trees and its unique lipid pattern, we propose that strains 313T and 352 represent members of a novel species in a new genus, for which the name Fudania jinshanensis gen. nov., sp.
31334694	9	33	theme	phosphatidylinositol	1092:1111	arg1	mannoside					1113:1121	phosphatidylinositol mannoside	1092:1121	phosphatidylinositol mannoside	1092:1121	The polar lipids comprised diphosphatidylglycerol, phosphatidylinositol mannoside, phosphatidylglycerol, phosphatidylinositol and five unidentified components.
31334694	14	34	theme	rRNA	1424:1427	arg1	pattern					1544:1550	its unique lipid pattern	1527:1550	its unique lipid pattern	1527:1550	Based on the low 16S rRNA gene sequence similarities, its taxonomic position in the phylogenetic and phylogenomic trees and its unique lipid pattern, we propose that strains 313T and 352 represent members of a novel species in a new genus, for which the name Fudania jinshanensis gen. nov., sp.
31334694	14	34	theme	rRNA	1424:1427	arg1	similarities					1443:1454	the low 16S rRNA gene sequence similarities	1412:1454	the low 16S rRNA gene sequence similarities	1412:1454	Based on the low 16S rRNA gene sequence similarities, its taxonomic position in the phylogenetic and phylogenomic trees and its unique lipid pattern, we propose that strains 313T and 352 represent members of a novel species in a new genus, for which the name Fudania jinshanensis gen. nov., sp.
31334694	14	34	theme	rRNA	1424:1427	arg1	position					1471:1478	its taxonomic position	1457:1478	its taxonomic position in the phylogenetic and phylogenomic trees	1457:1521	Based on the low 16S rRNA gene sequence similarities, its taxonomic position in the phylogenetic and phylogenomic trees and its unique lipid pattern, we propose that strains 313T and 352 represent members of a novel species in a new genus, for which the name Fudania jinshanensis gen. nov., sp.
31334694	2	35	from	faeces	197:202	arg1	China					258:262	the Tibet-Qinghai Plateau, PR China	228:262	China	258:262	Two hitherto unknown bacteria (strains 313T and 352) were recovered from the faeces of Tibetan antelopes on the Tibet-Qinghai Plateau, PR China.
31334694	14	36	theme	gene	1429:1432	arg1	pattern					1544:1550	its unique lipid pattern	1527:1550	its unique lipid pattern	1527:1550	Based on the low 16S rRNA gene sequence similarities, its taxonomic position in the phylogenetic and phylogenomic trees and its unique lipid pattern, we propose that strains 313T and 352 represent members of a novel species in a new genus, for which the name Fudania jinshanensis gen. nov., sp.
31334694	14	36	theme	gene	1429:1432	arg1	similarities					1443:1454	the low 16S rRNA gene sequence similarities	1412:1454	the low 16S rRNA gene sequence similarities	1412:1454	Based on the low 16S rRNA gene sequence similarities, its taxonomic position in the phylogenetic and phylogenomic trees and its unique lipid pattern, we propose that strains 313T and 352 represent members of a novel species in a new genus, for which the name Fudania jinshanensis gen. nov., sp.
31334694	14	36	theme	gene	1429:1432	arg1	position					1471:1478	its taxonomic position	1457:1478	its taxonomic position in the phylogenetic and phylogenomic trees	1457:1521	Based on the low 16S rRNA gene sequence similarities, its taxonomic position in the phylogenetic and phylogenomic trees and its unique lipid pattern, we propose that strains 313T and 352 represent members of a novel species in a new genus, for which the name Fudania jinshanensis gen. nov., sp.
31334694	8	37	theme	cellular	976:983	arg1	C16 					1017:1020	C16 	1017:1020	C16 	1017:1020	The major cellular fatty acids of the strains were C16 : 0 and C18 : 1ω9c.
31334694	8	37	theme	cellular	976:983	arg1	acids					991:995	The major cellular fatty acids	966:995	The major cellular fatty acids of the strains	966:1010	The major cellular fatty acids of the strains were C16 : 0 and C18 : 1ω9c.
31334694	7	38	theme	Phylogenomic	842:853	arg1	analysis					855:862	Phylogenomic analysis	842:862	Phylogenomic analysis	842:862	Phylogenomic analysis also indicated that strains 313T and 352 formed an independent branch in the family Actinomycetaceae.
31334694	1	39	theme	Tibetan	70:76	arg1	hodgsonii					99:107	Pantholops hodgsonii	88:107	Pantholops hodgsonii	88:107	nov., isolated from faeces of the Tibetan antelope (Pantholops hodgsonii) in China.
31334694	1	39	theme	Tibetan	70:76	arg1	antelope					78:85	the Tibetan antelope	66:85	the Tibetan antelope (Pantholops hodgsonii) in China	66:117	nov., isolated from faeces of the Tibetan antelope (Pantholops hodgsonii) in China.
31334694	16	40	theme	type	1720:1723	arg1	313T					1735:1738	313T	1735:1738	313T (=CGMCC 4.7453T=DSM 106216T)	1735:1767	The type strain is 313T (=CGMCC 4.7453T=DSM 106216T).
31334694	16	40	theme	type	1720:1723	arg1	strain					1725:1730	The type strain	1716:1730	The type strain	1716:1730	The type strain is 313T (=CGMCC 4.7453T=DSM 106216T).
31334694	13	41	theme	60.6 mol	1392:1399	arg1	G+C content					1361:1371	The DNA G+C content	1353:1371	The DNA G+C content of strain 313T	1353:1386	The DNA G+C content of strain 313T was 60.6 mol%.
31334694	13	41	theme	60.6 mol	1392:1399	arg1	%					1400:1400	60.6 mol%	1392:1400	60.6 mol%	1392:1400	The DNA G+C content of strain 313T was 60.6 mol%.
31334694	0	42	theme	gen.	21:24	arg1	nov.					26:29	Fudania jinshanensis gen. nov.	0:29	Fudania jinshanensis gen. nov.	0:29	Fudania jinshanensis gen. nov., sp.
31334694	8	43	theme	major	970:974	arg1	C16 					1017:1020	C16 	1017:1020	C16 	1017:1020	The major cellular fatty acids of the strains were C16 : 0 and C18 : 1ω9c.
31334694	8	43	theme	major	970:974	arg1	acids					991:995	The major cellular fatty acids	966:995	The major cellular fatty acids of the strains	966:1010	The major cellular fatty acids of the strains were C16 : 0 and C18 : 1ω9c.
31334694	14	44	theme	novel	1613:1617	arg1	species					1619:1625	a novel species	1611:1625	a novel species	1611:1625	Based on the low 16S rRNA gene sequence similarities, its taxonomic position in the phylogenetic and phylogenomic trees and its unique lipid pattern, we propose that strains 313T and 352 represent members of a novel species in a new genus, for which the name Fudania jinshanensis gen. nov., sp.
31334694	1	45	theme	antelope	78:85	arg1	faeces					56:61	faeces	56:61	faeces of the Tibetan antelope (Pantholops hodgsonii) in China	56:117	nov., isolated from faeces of the Tibetan antelope (Pantholops hodgsonii) in China.
31334694	6	46	dep	genera	718:723	arg1	Actinobaculum					739:751	Actinobaculum	739:751	Actinobaculum	739:751	Phylogenetic analyses showed that strains 313T and 352 clustered independently in the vicinity of the genera Actinotignum, Actinobaculum and Flaviflexus, but could not be classified clearly as a member of any of these genera.
31334694	6	46	dep	genera	718:723	arg1	Flaviflexus					757:767	Flaviflexus	757:767	Flaviflexus	757:767	Phylogenetic analyses showed that strains 313T and 352 clustered independently in the vicinity of the genera Actinotignum, Actinobaculum and Flaviflexus, but could not be classified clearly as a member of any of these genera.
31334694	16	47	theme	4.7453T=DSM	1748:1758	arg1	106216T					1760:1766	=CGMCC 4.7453T=DSM 106216T	1741:1766	=CGMCC 4.7453T=DSM 106216T	1741:1766	The type strain is 313T (=CGMCC 4.7453T=DSM 106216T).
31334694	16	47	theme	4.7453T=DSM	1748:1758	arg1	313T					1735:1738	313T	1735:1738	313T (=CGMCC 4.7453T=DSM 106216T)	1735:1767	The type strain is 313T (=CGMCC 4.7453T=DSM 106216T).
31334694	13	48	theme	strain	1376:1381	arg1	313T					1383:1386	strain 313T	1376:1386	strain 313T	1376:1386	The DNA G+C content of strain 313T was 60.6 mol%.
31334694	7	49	theme	family	941:946	arg1	Actinomycetaceae					948:963	the family Actinomycetaceae	937:963	the family Actinomycetaceae	937:963	Phylogenomic analysis also indicated that strains 313T and 352 formed an independent branch in the family Actinomycetaceae.
31334694	14	50	dep	sp	1694:1695	arg1	nov.					1688:1691	the name Fudania jinshanensis gen. nov.	1653:1691	the name Fudania jinshanensis gen. nov.	1653:1691	Based on the low 16S rRNA gene sequence similarities, its taxonomic position in the phylogenetic and phylogenomic trees and its unique lipid pattern, we propose that strains 313T and 352 represent members of a novel species in a new genus, for which the name Fudania jinshanensis gen. nov., sp.
31334694	14	51	theme	jinshanensis	1670:1681	arg1	nov.					1688:1691	the name Fudania jinshanensis gen. nov.	1653:1691	the name Fudania jinshanensis gen. nov.	1653:1691	Based on the low 16S rRNA gene sequence similarities, its taxonomic position in the phylogenetic and phylogenomic trees and its unique lipid pattern, we propose that strains 313T and 352 represent members of a novel species in a new genus, for which the name Fudania jinshanensis gen. nov., sp.
31334694	8	52	theme	fatty	985:989	arg1	C16 					1017:1020	C16 	1017:1020	C16 	1017:1020	The major cellular fatty acids of the strains were C16 : 0 and C18 : 1ω9c.
31334694	8	52	theme	fatty	985:989	arg1	acids					991:995	The major cellular fatty acids	966:995	The major cellular fatty acids of the strains	966:1010	The major cellular fatty acids of the strains were C16 : 0 and C18 : 1ω9c.
31334694	2	53	theme	unknown	133:139	arg1	bacteria					141:148	Two hitherto unknown bacteria	120:148	Two hitherto unknown bacteria (strains 313T and 352)	120:171	Two hitherto unknown bacteria (strains 313T and 352) were recovered from the faeces of Tibetan antelopes on the Tibet-Qinghai Plateau, PR China.
31334694	2	53	theme	unknown	133:139	arg1	strains					151:157	strains 313T and 352	151:170	strains 313T and 352	151:170	Two hitherto unknown bacteria (strains 313T and 352) were recovered from the faeces of Tibetan antelopes on the Tibet-Qinghai Plateau, PR China.
31334694	5	54	theme	Arcanobacterium	470:484	arg1	haemolyticum					486:497	Arcanobacterium haemolyticum	470:497	Arcanobacterium haemolyticum (92.5 %)	470:506	The isolates were closely related to Actinotignum sanguinis (92.6 % 16S rRNA gene sequence similarity), Arcanobacterium haemolyticum (92.5 %), Actinotignum schaalii (92.4 %), Actinobaculum massiliense (92.2 %) and Flaviflexus huanghaiensis (91.6 %).
31334694	5	54	theme	Arcanobacterium	470:484	arg1	%					505:505	92.5 %	500:505	92.5 %	500:505	The isolates were closely related to Actinotignum sanguinis (92.6 % 16S rRNA gene sequence similarity), Arcanobacterium haemolyticum (92.5 %), Actinotignum schaalii (92.4 %), Actinobaculum massiliense (92.2 %) and Flaviflexus huanghaiensis (91.6 %).
31334694	11	55	theme	respiratory	1268:1278	arg1	quinone					1280:1286	The respiratory quinone	1264:1286	The respiratory quinone	1264:1286	The respiratory quinone was absent.
31334694	11	55	theme	respiratory	1268:1278	arg1	absent					1292:1297	absent	1292:1297	absent	1292:1297	The respiratory quinone was absent.
31334694	14	56	theme	Fudania	1662:1668	arg1	nov.					1688:1691	the name Fudania jinshanensis gen. nov.	1653:1691	the name Fudania jinshanensis gen. nov.	1653:1691	Based on the low 16S rRNA gene sequence similarities, its taxonomic position in the phylogenetic and phylogenomic trees and its unique lipid pattern, we propose that strains 313T and 352 represent members of a novel species in a new genus, for which the name Fudania jinshanensis gen. nov., sp.
31334694	0	57	dep	sp	32:33	arg1	nov.					26:29	Fudania jinshanensis gen. nov.	0:29	Fudania jinshanensis gen. nov.	0:29	Fudania jinshanensis gen. nov., sp.
31334694	14	58	dep	strains	1569:1575	arg1	352					1586:1588	352	1586:1588	352	1586:1588	Based on the low 16S rRNA gene sequence similarities, its taxonomic position in the phylogenetic and phylogenomic trees and its unique lipid pattern, we propose that strains 313T and 352 represent members of a novel species in a new genus, for which the name Fudania jinshanensis gen. nov., sp.
31334694	14	58	dep	strains	1569:1575	arg1	313T					1577:1580	313T	1577:1580	313T	1577:1580	Based on the low 16S rRNA gene sequence similarities, its taxonomic position in the phylogenetic and phylogenomic trees and its unique lipid pattern, we propose that strains 313T and 352 represent members of a novel species in a new genus, for which the name Fudania jinshanensis gen. nov., sp.
31334694	6	59	dep	strains	650:656	arg1	313T					658:661	313T	658:661	313T	658:661	Phylogenetic analyses showed that strains 313T and 352 clustered independently in the vicinity of the genera Actinotignum, Actinobaculum and Flaviflexus, but could not be classified clearly as a member of any of these genera.
31334694	6	59	dep	strains	650:656	arg1	352					667:669	352	667:669	352	667:669	Phylogenetic analyses showed that strains 313T and 352 clustered independently in the vicinity of the genera Actinotignum, Actinobaculum and Flaviflexus, but could not be classified clearly as a member of any of these genera.
31334694	13	60	theme	313T	1383:1386	arg1	G+C content					1361:1371	The DNA G+C content	1353:1371	The DNA G+C content of strain 313T	1353:1386	The DNA G+C content of strain 313T was 60.6 mol%.
31334694	13	60	theme	313T	1383:1386	arg1	%					1400:1400	60.6 mol%	1392:1400	60.6 mol%	1392:1400	The DNA G+C content of strain 313T was 60.6 mol%.
31334694	1	61	theme	Pantholops	88:97	arg1	hodgsonii					99:107	Pantholops hodgsonii	88:107	Pantholops hodgsonii	88:107	nov., isolated from faeces of the Tibetan antelope (Pantholops hodgsonii) in China.
31334694	1	61	theme	Pantholops	88:97	arg1	antelope					78:85	the Tibetan antelope	66:85	the Tibetan antelope (Pantholops hodgsonii) in China	66:117	nov., isolated from faeces of the Tibetan antelope (Pantholops hodgsonii) in China.
31334694	14	62	theme	16S	1420:1422	arg1	pattern					1544:1550	its unique lipid pattern	1527:1550	its unique lipid pattern	1527:1550	Based on the low 16S rRNA gene sequence similarities, its taxonomic position in the phylogenetic and phylogenomic trees and its unique lipid pattern, we propose that strains 313T and 352 represent members of a novel species in a new genus, for which the name Fudania jinshanensis gen. nov., sp.
31334694	14	62	theme	16S	1420:1422	arg1	similarities					1443:1454	the low 16S rRNA gene sequence similarities	1412:1454	the low 16S rRNA gene sequence similarities	1412:1454	Based on the low 16S rRNA gene sequence similarities, its taxonomic position in the phylogenetic and phylogenomic trees and its unique lipid pattern, we propose that strains 313T and 352 represent members of a novel species in a new genus, for which the name Fudania jinshanensis gen. nov., sp.
31334694	14	62	theme	16S	1420:1422	arg1	position					1471:1478	its taxonomic position	1457:1478	its taxonomic position in the phylogenetic and phylogenomic trees	1457:1521	Based on the low 16S rRNA gene sequence similarities, its taxonomic position in the phylogenetic and phylogenomic trees and its unique lipid pattern, we propose that strains 313T and 352 represent members of a novel species in a new genus, for which the name Fudania jinshanensis gen. nov., sp.
31334694	6	63	theme	any	821:823	arg1	member					811:816	a member	809:816	a member of any of these genera	809:839	Phylogenetic analyses showed that strains 313T and 352 clustered independently in the vicinity of the genera Actinotignum, Actinobaculum and Flaviflexus, but could not be classified clearly as a member of any of these genera.
31334694	6	63	theme	any	821:823	arg1	analyses					629:636	Phylogenetic analyses	616:636	Phylogenetic analyses	616:636	Phylogenetic analyses showed that strains 313T and 352 clustered independently in the vicinity of the genera Actinotignum, Actinobaculum and Flaviflexus, but could not be classified clearly as a member of any of these genera.
31334694	7	64	theme	independent	915:925	arg1	branch					927:932	an independent branch	912:932	an independent branch in the family Actinomycetaceae	912:963	Phylogenomic analysis also indicated that strains 313T and 352 formed an independent branch in the family Actinomycetaceae.
31334694	2	65	theme	Tibetan	207:213	arg1	antelopes					215:223	Tibetan antelopes	207:223	Tibetan antelopes on the Tibet-Qinghai Plateau, PR China	207:262	Two hitherto unknown bacteria (strains 313T and 352) were recovered from the faeces of Tibetan antelopes on the Tibet-Qinghai Plateau, PR China.
31334694	10	66	theme	glutamic	1249:1256	arg1	acid					1258:1261	glutamic acid	1249:1261	glutamic acid	1249:1261	The peptidoglycan contained lysine, alanine and glutamic acid.
31334694	2	67	dep	China	258:262	arg1	PR					255:256	the Tibet-Qinghai Plateau, PR China	228:262	PR	255:256	Two hitherto unknown bacteria (strains 313T and 352) were recovered from the faeces of Tibetan antelopes on the Tibet-Qinghai Plateau, PR China.
31334694	1	68	from	antelope	78:85	arg1	China					113:117	China	113:117	China	113:117	nov., isolated from faeces of the Tibetan antelope (Pantholops hodgsonii) in China.
31334694	12	69	theme	whole-cell	1304:1313	arg1	sugars					1315:1320	The whole-cell sugars	1300:1320	The whole-cell sugars	1300:1320	The whole-cell sugars included glucose and rhamnose.
31334694	8	70	theme	strains	1004:1010	arg1	C16 					1017:1020	C16 	1017:1020	C16 	1017:1020	The major cellular fatty acids of the strains were C16 : 0 and C18 : 1ω9c.
31334694	8	70	theme	strains	1004:1010	arg1	acids					991:995	The major cellular fatty acids	966:995	The major cellular fatty acids of the strains	966:1010	The major cellular fatty acids of the strains were C16 : 0 and C18 : 1ω9c.
31334694	9	71	theme	unidentified	1176:1187	arg1	components					1189:1198	five unidentified components	1171:1198	five unidentified components	1171:1198	The polar lipids comprised diphosphatidylglycerol, phosphatidylinositol mannoside, phosphatidylglycerol, phosphatidylinositol and five unidentified components.
31334694	5	72	theme	sequence	448:455	arg1	similarity					457:466	92.6 % 16S rRNA gene sequence similarity	427:466	92.6 % 16S rRNA gene sequence similarity	427:466	The isolates were closely related to Actinotignum sanguinis (92.6 % 16S rRNA gene sequence similarity), Arcanobacterium haemolyticum (92.5 %), Actinotignum schaalii (92.4 %), Actinobaculum massiliense (92.2 %) and Flaviflexus huanghaiensis (91.6 %).
31334694	5	72	theme	sequence	448:455	arg1	sanguinis					416:424	Actinotignum sanguinis	403:424	Actinotignum sanguinis (92.6 % 16S rRNA gene sequence similarity)	403:467	The isolates were closely related to Actinotignum sanguinis (92.6 % 16S rRNA gene sequence similarity), Arcanobacterium haemolyticum (92.5 %), Actinotignum schaalii (92.4 %), Actinobaculum massiliense (92.2 %) and Flaviflexus huanghaiensis (91.6 %).
31334694	2	73	dep	strains	151:157	arg1	313T					159:162	313T	159:162	313T	159:162	Two hitherto unknown bacteria (strains 313T and 352) were recovered from the faeces of Tibetan antelopes on the Tibet-Qinghai Plateau, PR China.
31334694	2	73	dep	strains	151:157	arg1	352					168:170	352	168:170	352	168:170	Two hitherto unknown bacteria (strains 313T and 352) were recovered from the faeces of Tibetan antelopes on the Tibet-Qinghai Plateau, PR China.
31334694	14	74	theme	unique	1531:1536	arg1	pattern					1544:1550	its unique lipid pattern	1527:1550	its unique lipid pattern	1527:1550	Based on the low 16S rRNA gene sequence similarities, its taxonomic position in the phylogenetic and phylogenomic trees and its unique lipid pattern, we propose that strains 313T and 352 represent members of a novel species in a new genus, for which the name Fudania jinshanensis gen. nov., sp.
31334694	14	74	theme	unique	1531:1536	arg1	similarities					1443:1454	the low 16S rRNA gene sequence similarities	1412:1454	the low 16S rRNA gene sequence similarities	1412:1454	Based on the low 16S rRNA gene sequence similarities, its taxonomic position in the phylogenetic and phylogenomic trees and its unique lipid pattern, we propose that strains 313T and 352 represent members of a novel species in a new genus, for which the name Fudania jinshanensis gen. nov., sp.
31334694	14	75	theme	phylogenetic	1487:1498	arg1	trees					1517:1521	the phylogenetic and phylogenomic trees	1483:1521	trees	1517:1521	Based on the low 16S rRNA gene sequence similarities, its taxonomic position in the phylogenetic and phylogenomic trees and its unique lipid pattern, we propose that strains 313T and 352 represent members of a novel species in a new genus, for which the name Fudania jinshanensis gen. nov., sp.
31334694	2	76	from	antelopes	215:223	arg1	China					258:262	the Tibet-Qinghai Plateau, PR China	228:262	China	258:262	Two hitherto unknown bacteria (strains 313T and 352) were recovered from the faeces of Tibetan antelopes on the Tibet-Qinghai Plateau, PR China.
31334694	14	77	theme	phylogenomic	1504:1515	arg1	trees					1517:1521	the phylogenetic and phylogenomic trees	1483:1521	trees	1517:1521	Based on the low 16S rRNA gene sequence similarities, its taxonomic position in the phylogenetic and phylogenomic trees and its unique lipid pattern, we propose that strains 313T and 352 represent members of a novel species in a new genus, for which the name Fudania jinshanensis gen. nov., sp.
30711759	10	0	theme	DMOG	1279:1282	arg1	release					1268:1274	release	1268:1274	release of DMOG from developed hydrogel	1268:1306	Furthermore, in response to release of DMOG from developed hydrogel, cells showed enhanced capillary tube formation in vitro.
30711759	4	1	theme	Developed	568:576	arg1	conjugate					585:593	Developed HA-Cys conjugate	568:593	Developed HA-Cys conjugate	568:593	Developed HA-Cys conjugate was cross-linked using PDA nanoparticles via Michael-type addition reaction.
30711759	11	2	theme	cross-linked	1410:1421	arg1	hydrogel					1444:1451	PDA cross-linked thiol-functionalized hydrogel	1406:1451	PDA cross-linked thiol-functionalized hydrogel	1406:1451	Overall, these results demonstrate that PDA cross-linked thiol-functionalized hydrogel was developed in a facile manner under physiological conditions.
30711759	9	3	theme	cell	1194:1197	arg1	migration					1199:1207	enhanced endothelial cell migration	1173:1207	enhanced endothelial cell migration	1173:1207	Hydrogel microenvironment facilitated enhanced endothelial cell migration, proliferation and attachment.
30711759	3	4	theme	Thiol-functionalized	363:382	arg1	acid					395:398	Thiol-functionalized hyaluronic acid	363:398	Thiol-functionalized hyaluronic acid	363:398	Thiol-functionalized hyaluronic acid was developed using cysteamine and hyaluronic acid (HA-Cys) via 1-Ethyl-3-(3-Dimethylaminopropyl) Carbodiimide - N-hydroxysuccinimide (EDC-NHS) crosslinking chemistry.
30711759	11	5	theme	physiological	1492:1504	arg1	conditions					1506:1515	physiological conditions	1492:1515	physiological conditions	1492:1515	Overall, these results demonstrate that PDA cross-linked thiol-functionalized hydrogel was developed in a facile manner under physiological conditions.
30711759	5	6	contain	had	744:746	arg1	nanoparticles					684:696	Synthesized nanoparticles	672:696	Synthesized nanoparticles	672:696	Synthesized nanoparticles were monodisperse with size of 124 ± 8 nm and had spherical morphology.
30711759	5	6	contain	had	744:746	arg2	morphology					758:767	spherical morphology	748:767	spherical morphology	748:767	Synthesized nanoparticles were monodisperse with size of 124 ± 8 nm and had spherical morphology.
30711759	5	7	theme	Synthesized	672:682	arg1	nanoparticles					684:696	Synthesized nanoparticles	672:696	Synthesized nanoparticles	672:696	Synthesized nanoparticles were monodisperse with size of 124 ± 8 nm and had spherical morphology.
30711759	12	8	theme	drug	1596:1599	arg1	delivery					1601:1608	drug delivery	1596:1608	drug delivery	1596:1608	These developed hydrogels could be potentially used in tissue engineering and drug delivery.
30711759	2	9	theme	dopamine	328:335	arg1	self-polymerization					305:323	oxidative self-polymerization	295:323	oxidative self-polymerization of dopamine in water-ethanol mixture	295:360	Herein, we synthesized Polydopamine (PDA) nanoparticles via oxidative self-polymerization of dopamine in water-ethanol mixture.
30711759	6	10	with	crosslinking	858:869	arg1	nanoparticles					880:892	PDA nanoparticles	876:892	PDA nanoparticles	876:892	FTIR characterization confirmed successful synthesis of HA-Cys conjugate and subsequent crosslinking with PDA nanoparticles.
30711759	7	11	from	injectable	953:962	arg1	nature					967:972	nature	967:972	nature	967:972	Rheological characterization revealed that hydrogels were injectable in nature with good mechanical stability.
30711759	7	12	theme	mechanical	984:993	arg1	stability					995:1003	good mechanical stability	979:1003	good mechanical stability	979:1003	Rheological characterization revealed that hydrogels were injectable in nature with good mechanical stability.
30711759	6	13	theme	subsequent	847:856	arg1	crosslinking					858:869	subsequent crosslinking	847:869	subsequent crosslinking with PDA nanoparticles	847:892	FTIR characterization confirmed successful synthesis of HA-Cys conjugate and subsequent crosslinking with PDA nanoparticles.
30711759	3	14	theme	crosslinking	544:555	arg1	chemistry					557:565	N-hydroxysuccinimide (EDC-NHS) crosslinking chemistry	513:565	N-hydroxysuccinimide (EDC-NHS) crosslinking chemistry	513:565	Thiol-functionalized hyaluronic acid was developed using cysteamine and hyaluronic acid (HA-Cys) via 1-Ethyl-3-(3-Dimethylaminopropyl) Carbodiimide - N-hydroxysuccinimide (EDC-NHS) crosslinking chemistry.
30711759	5	15	theme	124 ± 8 nm	729:738	arg1	size					721:724	size	721:724	size of 124 ± 8 nm	721:738	Synthesized nanoparticles were monodisperse with size of 124 ± 8 nm and had spherical morphology.
30711759	7	16	with	injectable	953:962	arg1	stability					995:1003	good mechanical stability	979:1003	good mechanical stability	979:1003	Rheological characterization revealed that hydrogels were injectable in nature with good mechanical stability.
30711759	2	17	theme	oxidative	295:303	arg1	self-polymerization					305:323	oxidative self-polymerization	295:323	oxidative self-polymerization of dopamine in water-ethanol mixture	295:360	Herein, we synthesized Polydopamine (PDA) nanoparticles via oxidative self-polymerization of dopamine in water-ethanol mixture.
30711759	8	18	theme	PDA	1042:1044	arg1	nanoparticle					1046:1057	PDA nanoparticle	1042:1057	PDA nanoparticle	1042:1057	Dimethyloxalylglycine (DMOG) loaded PDA nanoparticle showed sustained drug release for period of 7 days from composite hydrogel.
30711759	12	19	theme	developed	1524:1532	arg1	hydrogels					1534:1542	These developed hydrogels	1518:1542	These developed hydrogels	1518:1542	These developed hydrogels could be potentially used in tissue engineering and drug delivery.
30711759	9	20	theme	Hydrogel	1135:1142	arg1	microenvironment					1144:1159	Hydrogel microenvironment	1135:1159	Hydrogel microenvironment	1135:1159	Hydrogel microenvironment facilitated enhanced endothelial cell migration, proliferation and attachment.
30711759	7	21	from	nature	967:972	arg1	injectable					953:962	injectable	953:962	injectable	953:962	Rheological characterization revealed that hydrogels were injectable in nature with good mechanical stability.
30711759	6	22	theme	conjugate	833:841	arg1	synthesis					813:821	successful synthesis	802:821	successful synthesis of HA-Cys conjugate	802:841	FTIR characterization confirmed successful synthesis of HA-Cys conjugate and subsequent crosslinking with PDA nanoparticles.
30711759	6	22	theme	conjugate	833:841	arg1	crosslinking					858:869	subsequent crosslinking	847:869	subsequent crosslinking with PDA nanoparticles	847:892	FTIR characterization confirmed successful synthesis of HA-Cys conjugate and subsequent crosslinking with PDA nanoparticles.
30711759	10	23	theme	developed	1289:1297	arg1	hydrogel					1299:1306	developed hydrogel	1289:1306	developed hydrogel	1289:1306	Furthermore, in response to release of DMOG from developed hydrogel, cells showed enhanced capillary tube formation in vitro.
30711759	3	24	theme	hyaluronic	384:393	arg1	acid					395:398	Thiol-functionalized hyaluronic acid	363:398	Thiol-functionalized hyaluronic acid	363:398	Thiol-functionalized hyaluronic acid was developed using cysteamine and hyaluronic acid (HA-Cys) via 1-Ethyl-3-(3-Dimethylaminopropyl) Carbodiimide - N-hydroxysuccinimide (EDC-NHS) crosslinking chemistry.
30711759	11	25	theme	PDA	1406:1408	arg1	hydrogel					1444:1451	PDA cross-linked thiol-functionalized hydrogel	1406:1451	PDA cross-linked thiol-functionalized hydrogel	1406:1451	Overall, these results demonstrate that PDA cross-linked thiol-functionalized hydrogel was developed in a facile manner under physiological conditions.
30711759	6	26	theme	HA-Cys	826:831	arg1	conjugate					833:841	HA-Cys conjugate	826:841	HA-Cys conjugate	826:841	FTIR characterization confirmed successful synthesis of HA-Cys conjugate and subsequent crosslinking with PDA nanoparticles.
30711759	0	27	theme	Nano	0:3	arg1	polydopamine					5:16	Nano polydopamine	0:16	Nano polydopamine	0:16	Nano polydopamine crosslinked thiol-functionalized hyaluronic acid hydrogel for angiogenic drug delivery.
30711759	4	28	theme	Michael-type	640:651	arg1	reaction					662:669	Michael-type addition reaction	640:669	Michael-type addition reaction	640:669	Developed HA-Cys conjugate was cross-linked using PDA nanoparticles via Michael-type addition reaction.
30711759	5	29	theme	spherical	748:756	arg1	morphology					758:767	spherical morphology	748:767	spherical morphology	748:767	Synthesized nanoparticles were monodisperse with size of 124 ± 8 nm and had spherical morphology.
30711759	2	30	theme	water-ethanol	340:352	arg1	mixture					354:360	water-ethanol mixture	340:360	water-ethanol mixture	340:360	Herein, we synthesized Polydopamine (PDA) nanoparticles via oxidative self-polymerization of dopamine in water-ethanol mixture.
30711759	3	31	theme	3-Dimethylaminopropyl	475:495	arg1	Carbodiimide					498:509	1-Ethyl-3-(3-Dimethylaminopropyl) Carbodiimide	464:509	1-Ethyl-3-(3-Dimethylaminopropyl) Carbodiimide	464:509	Thiol-functionalized hyaluronic acid was developed using cysteamine and hyaluronic acid (HA-Cys) via 1-Ethyl-3-(3-Dimethylaminopropyl) Carbodiimide - N-hydroxysuccinimide (EDC-NHS) crosslinking chemistry.
30711759	0	32	theme	thiol-functionalized	30:49	arg1	hydrogel					67:74	thiol-functionalized hyaluronic acid hydrogel	30:74	thiol-functionalized hyaluronic acid hydrogel for angiogenic drug delivery	30:103	Nano polydopamine crosslinked thiol-functionalized hyaluronic acid hydrogel for angiogenic drug delivery.
30711759	7	33	theme	good	979:982	arg1	stability					995:1003	good mechanical stability	979:1003	good mechanical stability	979:1003	Rheological characterization revealed that hydrogels were injectable in nature with good mechanical stability.
30711759	12	34	theme	tissue	1573:1578	arg1	engineering					1580:1590	tissue engineering	1573:1590	tissue engineering	1573:1590	These developed hydrogels could be potentially used in tissue engineering and drug delivery.
30711759	1	35	theme	polymeric	122:130	arg1	nanoparticles					146:158	polymeric network using nanoparticles	122:158	polymeric network using nanoparticles	122:158	Crosslinking of polymeric network using nanoparticles by physical or chemical method to obtain hydrogel is an emerging approach.
30711759	8	36	theme	drug	1076:1079	arg1	release					1081:1087	sustained drug release	1066:1087	sustained drug release	1066:1087	Dimethyloxalylglycine (DMOG) loaded PDA nanoparticle showed sustained drug release for period of 7 days from composite hydrogel.
30711759	1	37	theme	emerging	216:223	arg1	approach					225:232	an emerging approach	213:232	an emerging approach	213:232	Crosslinking of polymeric network using nanoparticles by physical or chemical method to obtain hydrogel is an emerging approach.
30711759	1	37	theme	emerging	216:223	arg1	Crosslinking					106:117	Crosslinking	106:117	Crosslinking of polymeric network using nanoparticles by physical or chemical method to obtain hydrogel	106:208	Crosslinking of polymeric network using nanoparticles by physical or chemical method to obtain hydrogel is an emerging approach.
30711759	3	38	theme	hyaluronic	435:444	arg1	acid					446:449	hyaluronic acid	435:449	hyaluronic acid (HA-Cys)	435:458	Thiol-functionalized hyaluronic acid was developed using cysteamine and hyaluronic acid (HA-Cys) via 1-Ethyl-3-(3-Dimethylaminopropyl) Carbodiimide - N-hydroxysuccinimide (EDC-NHS) crosslinking chemistry.
30711759	3	38	theme	hyaluronic	435:444	arg1	HA-Cys					452:457	HA-Cys	452:457	HA-Cys	452:457	Thiol-functionalized hyaluronic acid was developed using cysteamine and hyaluronic acid (HA-Cys) via 1-Ethyl-3-(3-Dimethylaminopropyl) Carbodiimide - N-hydroxysuccinimide (EDC-NHS) crosslinking chemistry.
30711759	10	39	theme	enhanced	1322:1329	arg1	formation					1346:1354	enhanced capillary tube formation	1322:1354	enhanced capillary tube formation	1322:1354	Furthermore, in response to release of DMOG from developed hydrogel, cells showed enhanced capillary tube formation in vitro.
30711759	1	40	theme	network	132:138	arg1	nanoparticles					146:158	polymeric network using nanoparticles	122:158	polymeric network using nanoparticles	122:158	Crosslinking of polymeric network using nanoparticles by physical or chemical method to obtain hydrogel is an emerging approach.
30711759	6	41	theme	successful	802:811	arg1	synthesis					813:821	successful synthesis	802:821	successful synthesis of HA-Cys conjugate	802:841	FTIR characterization confirmed successful synthesis of HA-Cys conjugate and subsequent crosslinking with PDA nanoparticles.
30711759	3	42	theme	EDC-NHS	535:541	arg1	chemistry					557:565	N-hydroxysuccinimide (EDC-NHS) crosslinking chemistry	513:565	N-hydroxysuccinimide (EDC-NHS) crosslinking chemistry	513:565	Thiol-functionalized hyaluronic acid was developed using cysteamine and hyaluronic acid (HA-Cys) via 1-Ethyl-3-(3-Dimethylaminopropyl) Carbodiimide - N-hydroxysuccinimide (EDC-NHS) crosslinking chemistry.
30711759	0	43	theme	acid	62:65	arg1	hydrogel					67:74	thiol-functionalized hyaluronic acid hydrogel	30:74	thiol-functionalized hyaluronic acid hydrogel for angiogenic drug delivery	30:103	Nano polydopamine crosslinked thiol-functionalized hyaluronic acid hydrogel for angiogenic drug delivery.
30711759	4	44	theme	addition	653:660	arg1	reaction					662:669	Michael-type addition reaction	640:669	Michael-type addition reaction	640:669	Developed HA-Cys conjugate was cross-linked using PDA nanoparticles via Michael-type addition reaction.
30711759	11	45	theme	thiol-functionalized	1423:1442	arg1	hydrogel					1444:1451	PDA cross-linked thiol-functionalized hydrogel	1406:1451	PDA cross-linked thiol-functionalized hydrogel	1406:1451	Overall, these results demonstrate that PDA cross-linked thiol-functionalized hydrogel was developed in a facile manner under physiological conditions.
30711759	2	46	theme	Polydopamine	258:269	arg1	nanoparticles					277:289	Polydopamine (PDA) nanoparticles	258:289	Polydopamine (PDA) nanoparticles	258:289	Herein, we synthesized Polydopamine (PDA) nanoparticles via oxidative self-polymerization of dopamine in water-ethanol mixture.
30711759	8	47	theme	composite	1115:1123	arg1	hydrogel					1125:1132	composite hydrogel	1115:1132	composite hydrogel	1115:1132	Dimethyloxalylglycine (DMOG) loaded PDA nanoparticle showed sustained drug release for period of 7 days from composite hydrogel.
30711759	8	48	from	hydrogel	1125:1132	arg1	period					1093:1098	period	1093:1098	period of 7 days from composite hydrogel	1093:1132	Dimethyloxalylglycine (DMOG) loaded PDA nanoparticle showed sustained drug release for period of 7 days from composite hydrogel.
30711759	0	49	theme	hyaluronic	51:60	arg1	hydrogel					67:74	thiol-functionalized hyaluronic acid hydrogel	30:74	thiol-functionalized hyaluronic acid hydrogel for angiogenic drug delivery	30:103	Nano polydopamine crosslinked thiol-functionalized hyaluronic acid hydrogel for angiogenic drug delivery.
30711759	3	50	theme	1-Ethyl-3-	464:473	arg1	Carbodiimide					498:509	1-Ethyl-3-(3-Dimethylaminopropyl) Carbodiimide	464:509	1-Ethyl-3-(3-Dimethylaminopropyl) Carbodiimide	464:509	Thiol-functionalized hyaluronic acid was developed using cysteamine and hyaluronic acid (HA-Cys) via 1-Ethyl-3-(3-Dimethylaminopropyl) Carbodiimide - N-hydroxysuccinimide (EDC-NHS) crosslinking chemistry.
30711759	9	51	theme	enhanced	1173:1180	arg1	migration					1199:1207	enhanced endothelial cell migration	1173:1207	enhanced endothelial cell migration	1173:1207	Hydrogel microenvironment facilitated enhanced endothelial cell migration, proliferation and attachment.
30711759	3	52	theme	N-hydroxysuccinimide	513:532	arg1	chemistry					557:565	N-hydroxysuccinimide (EDC-NHS) crosslinking chemistry	513:565	N-hydroxysuccinimide (EDC-NHS) crosslinking chemistry	513:565	Thiol-functionalized hyaluronic acid was developed using cysteamine and hyaluronic acid (HA-Cys) via 1-Ethyl-3-(3-Dimethylaminopropyl) Carbodiimide - N-hydroxysuccinimide (EDC-NHS) crosslinking chemistry.
30711759	10	53	from	hydrogel	1299:1306	arg1	release					1268:1274	release	1268:1274	release of DMOG from developed hydrogel	1268:1306	Furthermore, in response to release of DMOG from developed hydrogel, cells showed enhanced capillary tube formation in vitro.
30711759	10	54	dep	release	1268:1274	arg1	response					1256:1263	response	1256:1263	response	1256:1263	Furthermore, in response to release of DMOG from developed hydrogel, cells showed enhanced capillary tube formation in vitro.
30711759	11	55	theme	facile	1472:1477	arg1	manner					1479:1484	a facile manner	1470:1484	a facile manner	1470:1484	Overall, these results demonstrate that PDA cross-linked thiol-functionalized hydrogel was developed in a facile manner under physiological conditions.
30711759	10	56	theme	capillary	1331:1339	arg1	formation					1346:1354	enhanced capillary tube formation	1322:1354	enhanced capillary tube formation	1322:1354	Furthermore, in response to release of DMOG from developed hydrogel, cells showed enhanced capillary tube formation in vitro.
30711759	11	57	link	cross-linked	1410:1421	arg1	hydrogel					1444:1451	PDA cross-linked thiol-functionalized hydrogel	1406:1451	PDA cross-linked thiol-functionalized hydrogel	1406:1451	Overall, these results demonstrate that PDA cross-linked thiol-functionalized hydrogel was developed in a facile manner under physiological conditions.
30711759	1	58	theme	using	140:144	arg1	nanoparticles					146:158	polymeric network using nanoparticles	122:158	polymeric network using nanoparticles	122:158	Crosslinking of polymeric network using nanoparticles by physical or chemical method to obtain hydrogel is an emerging approach.
30711759	5	59	with	monodisperse	703:714	arg1	size					721:724	size	721:724	size of 124 ± 8 nm	721:738	Synthesized nanoparticles were monodisperse with size of 124 ± 8 nm and had spherical morphology.
30711759	1	60	theme	nanoparticles	146:158	arg1	approach					225:232	an emerging approach	213:232	an emerging approach	213:232	Crosslinking of polymeric network using nanoparticles by physical or chemical method to obtain hydrogel is an emerging approach.
30711759	1	60	theme	nanoparticles	146:158	arg1	Crosslinking					106:117	Crosslinking	106:117	Crosslinking of polymeric network using nanoparticles by physical or chemical method to obtain hydrogel	106:208	Crosslinking of polymeric network using nanoparticles by physical or chemical method to obtain hydrogel is an emerging approach.
30711759	4	61	theme	PDA	618:620	arg1	nanoparticles					622:634	PDA nanoparticles	618:634	PDA nanoparticles	618:634	Developed HA-Cys conjugate was cross-linked using PDA nanoparticles via Michael-type addition reaction.
30711759	10	62	theme	tube	1341:1344	arg1	formation					1346:1354	enhanced capillary tube formation	1322:1354	enhanced capillary tube formation	1322:1354	Furthermore, in response to release of DMOG from developed hydrogel, cells showed enhanced capillary tube formation in vitro.
30711759	8	63	theme	days	1105:1108	arg1	period					1093:1098	period	1093:1098	period of 7 days from composite hydrogel	1093:1132	Dimethyloxalylglycine (DMOG) loaded PDA nanoparticle showed sustained drug release for period of 7 days from composite hydrogel.
30711759	6	64	theme	FTIR	770:773	arg1	characterization					775:790	FTIR characterization	770:790	FTIR characterization	770:790	FTIR characterization confirmed successful synthesis of HA-Cys conjugate and subsequent crosslinking with PDA nanoparticles.
30711759	6	65	with	synthesis	813:821	arg1	nanoparticles					880:892	PDA nanoparticles	876:892	PDA nanoparticles	876:892	FTIR characterization confirmed successful synthesis of HA-Cys conjugate and subsequent crosslinking with PDA nanoparticles.
30711759	1	66	theme	physical	163:170	arg1	method					184:189	physical or chemical method	163:189	physical or chemical method to obtain hydrogel	163:208	Crosslinking of polymeric network using nanoparticles by physical or chemical method to obtain hydrogel is an emerging approach.
30711759	8	67	theme	sustained	1066:1074	arg1	release					1081:1087	sustained drug release	1066:1087	sustained drug release	1066:1087	Dimethyloxalylglycine (DMOG) loaded PDA nanoparticle showed sustained drug release for period of 7 days from composite hydrogel.
30711759	0	68	theme	drug	91:94	arg1	delivery					96:103	angiogenic drug delivery	80:103	angiogenic drug delivery	80:103	Nano polydopamine crosslinked thiol-functionalized hyaluronic acid hydrogel for angiogenic drug delivery.
30711759	2	69	theme	PDA	272:274	arg1	nanoparticles					277:289	Polydopamine (PDA) nanoparticles	258:289	Polydopamine (PDA) nanoparticles	258:289	Herein, we synthesized Polydopamine (PDA) nanoparticles via oxidative self-polymerization of dopamine in water-ethanol mixture.
30711759	6	70	theme	PDA	876:878	arg1	nanoparticles					880:892	PDA nanoparticles	876:892	PDA nanoparticles	876:892	FTIR characterization confirmed successful synthesis of HA-Cys conjugate and subsequent crosslinking with PDA nanoparticles.
30711759	12	71	used	used	1565:1568	arg2	hydrogels					1534:1542	These developed hydrogels	1518:1542	These developed hydrogels	1518:1542	These developed hydrogels could be potentially used in tissue engineering and drug delivery.
30711759	2	72	from	self-polymerization	305:323	arg1	mixture					354:360	water-ethanol mixture	340:360	water-ethanol mixture	340:360	Herein, we synthesized Polydopamine (PDA) nanoparticles via oxidative self-polymerization of dopamine in water-ethanol mixture.
30711759	0	73	theme	angiogenic	80:89	arg1	delivery					96:103	angiogenic drug delivery	80:103	angiogenic drug delivery	80:103	Nano polydopamine crosslinked thiol-functionalized hyaluronic acid hydrogel for angiogenic drug delivery.
30711759	4	74	theme	HA-Cys	578:583	arg1	conjugate					585:593	Developed HA-Cys conjugate	568:593	Developed HA-Cys conjugate	568:593	Developed HA-Cys conjugate was cross-linked using PDA nanoparticles via Michael-type addition reaction.
30711759	9	75	theme	endothelial	1182:1192	arg1	migration					1199:1207	enhanced endothelial cell migration	1173:1207	enhanced endothelial cell migration	1173:1207	Hydrogel microenvironment facilitated enhanced endothelial cell migration, proliferation and attachment.
30711759	1	76	theme	chemical	175:182	arg1	method					184:189	physical or chemical method	163:189	physical or chemical method to obtain hydrogel	163:208	Crosslinking of polymeric network using nanoparticles by physical or chemical method to obtain hydrogel is an emerging approach.
30711759	7	77	theme	Rheological	895:905	arg1	characterization					907:922	Rheological characterization	895:922	Rheological characterization	895:922	Rheological characterization revealed that hydrogels were injectable in nature with good mechanical stability.
31287396	5	0	theme	molecular	1303:1311	arg1	phylogeny					1313:1321	molecular phylogeny	1303:1321	molecular phylogeny	1303:1321	Strain S5-TSA-19T had a distinctive chemotaxonomic pattern comprising A4α type peptidoglycan based on l-Lys-d-Asp, iso-C15 : 0 as the major fatty acid, absence of phosphatidylethanolamine as a major lipid and MK-7 and MK-6 as the major menaquinones, differentiating it from the genera Planococcus and Planomicrobium, thus supporting the findings of molecular phylogeny.
31287396	6	1	theme	strain	1333:1338	arg1	S5-TSA-19T					1340:1349	strain S5-TSA-19T	1333:1349	strain S5-TSA-19T	1333:1349	Further, strain S5-TSA-19T was able to biotransform hexahydro-1,3,5,-trinitro-1,2,5-triazine (RDX) into nitrite derivatives under aerobic conditions in 2-4 days, whereas the closest reference strains did not possess this property.
31287396	4	2	theme	rRNA	768:771	arg1	gene					773:776	16S rRNA gene	764:776	16S rRNA gene	764:776	Phylogenetic analysis based on 16S rRNA gene and whole-genome sequences (based on a conserved set of 400 proteins) retrieved the strain in a distinct branch indicating a separate lineage within the family Planococcaceae.
31287396	7	3	theme	novel	1693:1697	arg1	species					1699:1705	a novel species	1691:1705	a novel species in a novel genus for which the name Indiicoccus explosivorum gen. nov., sp	1691:1780	On the basis of polyphasic taxonomic characterization and a phylogenomics approach, strain S5-TSA-19T is proposed as the type strain of a novel species in a novel genus for which the name Indiicoccus explosivorum gen. nov., sp.
31287396	5	4	theme	fatty	1094:1098	arg1	acid					1100:1103	the major fatty acid	1084:1103	the major fatty acid	1084:1103	Strain S5-TSA-19T had a distinctive chemotaxonomic pattern comprising A4α type peptidoglycan based on l-Lys-d-Asp, iso-C15 : 0 as the major fatty acid, absence of phosphatidylethanolamine as a major lipid and MK-7 and MK-6 as the major menaquinones, differentiating it from the genera Planococcus and Planomicrobium, thus supporting the findings of molecular phylogeny.
31287396	7	5	theme	type	1676:1679	arg1	S5-TSA-19T					1646:1655	strain S5-TSA-19T	1639:1655	strain S5-TSA-19T	1639:1655	On the basis of polyphasic taxonomic characterization and a phylogenomics approach, strain S5-TSA-19T is proposed as the type strain of a novel species in a novel genus for which the name Indiicoccus explosivorum gen. nov., sp.
31287396	7	5	theme	type	1676:1679	arg1	strain					1681:1686	the type strain	1672:1686	the type strain of a novel species in a novel genus for which the name Indiicoccus explosivorum gen. nov., sp	1672:1780	On the basis of polyphasic taxonomic characterization and a phylogenomics approach, strain S5-TSA-19T is proposed as the type strain of a novel species in a novel genus for which the name Indiicoccus explosivorum gen. nov., sp.
31287396	5	6	theme	Strain	954:959	arg1	S5-TSA-19T					961:970	Strain S5-TSA-19T	954:970	Strain S5-TSA-19T	954:970	Strain S5-TSA-19T had a distinctive chemotaxonomic pattern comprising A4α type peptidoglycan based on l-Lys-d-Asp, iso-C15 : 0 as the major fatty acid, absence of phosphatidylethanolamine as a major lipid and MK-7 and MK-6 as the major menaquinones, differentiating it from the genera Planococcus and Planomicrobium, thus supporting the findings of molecular phylogeny.
31287396	8	7	theme	=JCM	1801:1804	arg1	12608T					1830:1835	=JCM 31737T=KCTC 33871T=MTCC 12608T	1801:1835	=JCM 31737T=KCTC 33871T=MTCC 12608T	1801:1835	nov. is proposed (=JCM 31737T=KCTC 33871T=MTCC 12608T).
31287396	7	8	dep	characterization	1592:1607	arg1	the					1558:1560	the	1558:1560	the	1558:1560	On the basis of polyphasic taxonomic characterization and a phylogenomics approach, strain S5-TSA-19T is proposed as the type strain of a novel species in a novel genus for which the name Indiicoccus explosivorum gen. nov., sp.
31287396	7	8	dep	characterization	1592:1607	arg1	basis					1562:1566	basis	1562:1566	basis	1562:1566	On the basis of polyphasic taxonomic characterization and a phylogenomics approach, strain S5-TSA-19T is proposed as the type strain of a novel species in a novel genus for which the name Indiicoccus explosivorum gen. nov., sp.
31287396	8	9	theme	33871T=MTCC	1818:1828	arg1	12608T					1830:1835	=JCM 31737T=KCTC 33871T=MTCC 12608T	1801:1835	=JCM 31737T=KCTC 33871T=MTCC 12608T	1801:1835	nov. is proposed (=JCM 31737T=KCTC 33871T=MTCC 12608T).
31287396	3	10	theme	sequencing	304:313	arg1	analysis					321:328	The 16S rRNA gene sequencing blast analysis	286:328	The 16S rRNA gene sequencing blast analysis	286:328	The 16S rRNA gene sequencing blast analysis indicated that the strain is a member of the family Planococcaceae with the highest sequence similarity to Planomicrobium soli XN13T (96.1 %), followed by Planococcus maitriensis S1T (95.6 %), Planococcus plakortidis DSM 23997T (95.6 %), Planomicrobium flavidum ISL-41T (95.6 %), Planococcus rifietoensis M8T (95.5 %), Planococcus salinus LCB217T (95.5 %) and Planococcus maritimus DSM 17275T (95.5 %).
31287396	2	11	theme	explosives	227:236	arg1	site					251:254	an explosives contaminated site	224:254	an explosives contaminated site in Panchkula, Haryana, India	224:283	A pink-pigmented, Gram-stain-positive, aerobic, coccoid-shaped bacterial strain, designated as S5-TSA-19T, was isolated from an explosives contaminated site in Panchkula, Haryana, India.
31287396	6	12	theme	aerobic	1454:1460	arg1	conditions					1462:1471	aerobic conditions	1454:1471	aerobic conditions in 2-4 days	1454:1483	Further, strain S5-TSA-19T was able to biotransform hexahydro-1,3,5,-trinitro-1,2,5-triazine (RDX) into nitrite derivatives under aerobic conditions in 2-4 days, whereas the closest reference strains did not possess this property.
31287396	7	13	theme	phylogenomics	1615:1627	arg1	approach					1629:1636	a phylogenomics approach	1613:1636	a phylogenomics approach	1613:1636	On the basis of polyphasic taxonomic characterization and a phylogenomics approach, strain S5-TSA-19T is proposed as the type strain of a novel species in a novel genus for which the name Indiicoccus explosivorum gen. nov., sp.
31287396	3	14	theme	rRNA	294:297	arg1	analysis					321:328	The 16S rRNA gene sequencing blast analysis	286:328	The 16S rRNA gene sequencing blast analysis	286:328	The 16S rRNA gene sequencing blast analysis indicated that the strain is a member of the family Planococcaceae with the highest sequence similarity to Planomicrobium soli XN13T (96.1 %), followed by Planococcus maitriensis S1T (95.6 %), Planococcus plakortidis DSM 23997T (95.6 %), Planomicrobium flavidum ISL-41T (95.6 %), Planococcus rifietoensis M8T (95.5 %), Planococcus salinus LCB217T (95.5 %) and Planococcus maritimus DSM 17275T (95.5 %).
31287396	5	15	theme	A4α	1024:1026	arg1	peptidoglycan					1033:1045	A4α type peptidoglycan	1024:1045	A4α type peptidoglycan	1024:1045	Strain S5-TSA-19T had a distinctive chemotaxonomic pattern comprising A4α type peptidoglycan based on l-Lys-d-Asp, iso-C15 : 0 as the major fatty acid, absence of phosphatidylethanolamine as a major lipid and MK-7 and MK-6 as the major menaquinones, differentiating it from the genera Planococcus and Planomicrobium, thus supporting the findings of molecular phylogeny.
31287396	6	16	from	conditions	1462:1471	arg1	2-4 days					1476:1483	2-4 days	1476:1483	2-4 days	1476:1483	Further, strain S5-TSA-19T was able to biotransform hexahydro-1,3,5,-trinitro-1,2,5-triazine (RDX) into nitrite derivatives under aerobic conditions in 2-4 days, whereas the closest reference strains did not possess this property.
31287396	7	17	dep	name	1738:1741	arg1	nov.					1773:1776	Indiicoccus explosivorum gen. nov.	1743:1776	the name Indiicoccus explosivorum gen. nov.	1734:1776	On the basis of polyphasic taxonomic characterization and a phylogenomics approach, strain S5-TSA-19T is proposed as the type strain of a novel species in a novel genus for which the name Indiicoccus explosivorum gen. nov., sp.
31287396	5	18	dep	iso-C15 	1069:1076	arg1	 0					1078:1079	 0	1078:1079	 0 as the major fatty acid	1078:1103	Strain S5-TSA-19T had a distinctive chemotaxonomic pattern comprising A4α type peptidoglycan based on l-Lys-d-Asp, iso-C15 : 0 as the major fatty acid, absence of phosphatidylethanolamine as a major lipid and MK-7 and MK-6 as the major menaquinones, differentiating it from the genera Planococcus and Planomicrobium, thus supporting the findings of molecular phylogeny.
31287396	5	19	theme	major	1147:1151	arg1	lipid					1153:1157	a major lipid	1145:1157	a major lipid	1145:1157	Strain S5-TSA-19T had a distinctive chemotaxonomic pattern comprising A4α type peptidoglycan based on l-Lys-d-Asp, iso-C15 : 0 as the major fatty acid, absence of phosphatidylethanolamine as a major lipid and MK-7 and MK-6 as the major menaquinones, differentiating it from the genera Planococcus and Planomicrobium, thus supporting the findings of molecular phylogeny.
31287396	7	20	theme	taxonomic	1582:1590	arg1	characterization					1592:1607	polyphasic taxonomic characterization	1571:1607	polyphasic taxonomic characterization	1571:1607	On the basis of polyphasic taxonomic characterization and a phylogenomics approach, strain S5-TSA-19T is proposed as the type strain of a novel species in a novel genus for which the name Indiicoccus explosivorum gen. nov., sp.
31287396	2	21	attach	isolated	210:217	arg1	site					251:254	an explosives contaminated site	224:254	an explosives contaminated site in Panchkula, Haryana, India	224:283	A pink-pigmented, Gram-stain-positive, aerobic, coccoid-shaped bacterial strain, designated as S5-TSA-19T, was isolated from an explosives contaminated site in Panchkula, Haryana, India.
31287396	2	21	attach	isolated	210:217	arg2	strain					172:177	A pink-pigmented, Gram-stain-positive, aerobic, coccoid-shaped bacterial strain	99:177	A pink-pigmented, Gram-stain-positive, aerobic, coccoid-shaped bacterial strain	99:177	A pink-pigmented, Gram-stain-positive, aerobic, coccoid-shaped bacterial strain, designated as S5-TSA-19T, was isolated from an explosives contaminated site in Panchkula, Haryana, India.
31287396	3	22	theme	highest	406:412	arg1	similarity					423:432	the highest sequence similarity	402:432	the highest sequence similarity to Planomicrobium soli XN13T (96.1 %)	402:470	The 16S rRNA gene sequencing blast analysis indicated that the strain is a member of the family Planococcaceae with the highest sequence similarity to Planomicrobium soli XN13T (96.1 %), followed by Planococcus maitriensis S1T (95.6 %), Planococcus plakortidis DSM 23997T (95.6 %), Planomicrobium flavidum ISL-41T (95.6 %), Planococcus rifietoensis M8T (95.5 %), Planococcus salinus LCB217T (95.5 %) and Planococcus maritimus DSM 17275T (95.5 %).
31287396	5	23	theme	distinctive	978:988	arg1	pattern					1005:1011	a distinctive chemotaxonomic pattern	976:1011	a distinctive chemotaxonomic pattern comprising A4α type peptidoglycan	976:1045	Strain S5-TSA-19T had a distinctive chemotaxonomic pattern comprising A4α type peptidoglycan based on l-Lys-d-Asp, iso-C15 : 0 as the major fatty acid, absence of phosphatidylethanolamine as a major lipid and MK-7 and MK-6 as the major menaquinones, differentiating it from the genera Planococcus and Planomicrobium, thus supporting the findings of molecular phylogeny.
31287396	0	24	theme	Indiicoccus	0:10	arg1	nov.					30:33	Indiicoccus explosivorum gen. nov.	0:33	Indiicoccus explosivorum gen. nov.	0:33	Indiicoccus explosivorum gen. nov., sp.
31287396	6	25	theme	reference	1506:1514	arg1	strains					1516:1522	the closest reference strains	1494:1522	the closest reference strains	1494:1522	Further, strain S5-TSA-19T was able to biotransform hexahydro-1,3,5,-trinitro-1,2,5-triazine (RDX) into nitrite derivatives under aerobic conditions in 2-4 days, whereas the closest reference strains did not possess this property.
31287396	1	26	theme	waste	74:78	arg1	site					93:96	an explosives waste contaminated site	60:96	an explosives waste contaminated site	60:96	nov., isolated from an explosives waste contaminated site.
31287396	4	27	theme	separate	903:910	arg1	lineage					912:918	a separate lineage	901:918	a separate lineage within the family Planococcaceae	901:951	Phylogenetic analysis based on 16S rRNA gene and whole-genome sequences (based on a conserved set of 400 proteins) retrieved the strain in a distinct branch indicating a separate lineage within the family Planococcaceae.
31287396	3	28	theme	Planomicrobium	437:450	arg1	XN13T					457:461	Planomicrobium soli XN13T	437:461	Planomicrobium soli XN13T (96.1 %)	437:470	The 16S rRNA gene sequencing blast analysis indicated that the strain is a member of the family Planococcaceae with the highest sequence similarity to Planomicrobium soli XN13T (96.1 %), followed by Planococcus maitriensis S1T (95.6 %), Planococcus plakortidis DSM 23997T (95.6 %), Planomicrobium flavidum ISL-41T (95.6 %), Planococcus rifietoensis M8T (95.5 %), Planococcus salinus LCB217T (95.5 %) and Planococcus maritimus DSM 17275T (95.5 %).
31287396	3	28	theme	Planomicrobium	437:450	arg1	%					469:469	96.1 %	464:469	96.1 %	464:469	The 16S rRNA gene sequencing blast analysis indicated that the strain is a member of the family Planococcaceae with the highest sequence similarity to Planomicrobium soli XN13T (96.1 %), followed by Planococcus maitriensis S1T (95.6 %), Planococcus plakortidis DSM 23997T (95.6 %), Planomicrobium flavidum ISL-41T (95.6 %), Planococcus rifietoensis M8T (95.5 %), Planococcus salinus LCB217T (95.5 %) and Planococcus maritimus DSM 17275T (95.5 %).
31287396	0	29	theme	gen.	25:28	arg1	nov.					30:33	Indiicoccus explosivorum gen. nov.	0:33	Indiicoccus explosivorum gen. nov.	0:33	Indiicoccus explosivorum gen. nov., sp.
31287396	3	30	theme	family	375:380	arg1	Planococcaceae					382:395	the family Planococcaceae	371:395	the family Planococcaceae	371:395	The 16S rRNA gene sequencing blast analysis indicated that the strain is a member of the family Planococcaceae with the highest sequence similarity to Planomicrobium soli XN13T (96.1 %), followed by Planococcus maitriensis S1T (95.6 %), Planococcus plakortidis DSM 23997T (95.6 %), Planomicrobium flavidum ISL-41T (95.6 %), Planococcus rifietoensis M8T (95.5 %), Planococcus salinus LCB217T (95.5 %) and Planococcus maritimus DSM 17275T (95.5 %).
31287396	5	31	theme	major	1184:1188	arg1	menaquinones					1190:1201	the major menaquinones	1180:1201	the major menaquinones	1180:1201	Strain S5-TSA-19T had a distinctive chemotaxonomic pattern comprising A4α type peptidoglycan based on l-Lys-d-Asp, iso-C15 : 0 as the major fatty acid, absence of phosphatidylethanolamine as a major lipid and MK-7 and MK-6 as the major menaquinones, differentiating it from the genera Planococcus and Planomicrobium, thus supporting the findings of molecular phylogeny.
31287396	8	32	dep	proposed	1791:1798	arg1	12608T					1830:1835	=JCM 31737T=KCTC 33871T=MTCC 12608T	1801:1835	=JCM 31737T=KCTC 33871T=MTCC 12608T	1801:1835	nov. is proposed (=JCM 31737T=KCTC 33871T=MTCC 12608T).
31287396	4	33	theme	distinct	874:881	arg1	branch					883:888	a distinct branch	872:888	a distinct branch	872:888	Phylogenetic analysis based on 16S rRNA gene and whole-genome sequences (based on a conserved set of 400 proteins) retrieved the strain in a distinct branch indicating a separate lineage within the family Planococcaceae.
31287396	3	34	theme	Planomicrobium	568:581	arg1	ISL-41T					592:598	Planomicrobium flavidum ISL-41T	568:598	Planomicrobium flavidum ISL-41T (95.6 %)	568:607	The 16S rRNA gene sequencing blast analysis indicated that the strain is a member of the family Planococcaceae with the highest sequence similarity to Planomicrobium soli XN13T (96.1 %), followed by Planococcus maitriensis S1T (95.6 %), Planococcus plakortidis DSM 23997T (95.6 %), Planomicrobium flavidum ISL-41T (95.6 %), Planococcus rifietoensis M8T (95.5 %), Planococcus salinus LCB217T (95.5 %) and Planococcus maritimus DSM 17275T (95.5 %).
31287396	3	34	theme	Planomicrobium	568:581	arg1	%					606:606	95.6 %	601:606	95.6 %	601:606	The 16S rRNA gene sequencing blast analysis indicated that the strain is a member of the family Planococcaceae with the highest sequence similarity to Planomicrobium soli XN13T (96.1 %), followed by Planococcus maitriensis S1T (95.6 %), Planococcus plakortidis DSM 23997T (95.6 %), Planomicrobium flavidum ISL-41T (95.6 %), Planococcus rifietoensis M8T (95.5 %), Planococcus salinus LCB217T (95.5 %) and Planococcus maritimus DSM 17275T (95.5 %).
31287396	7	35	theme	gen.	1768:1771	arg1	nov.					1773:1776	Indiicoccus explosivorum gen. nov.	1743:1776	the name Indiicoccus explosivorum gen. nov.	1734:1776	On the basis of polyphasic taxonomic characterization and a phylogenomics approach, strain S5-TSA-19T is proposed as the type strain of a novel species in a novel genus for which the name Indiicoccus explosivorum gen. nov., sp.
31287396	5	36	dep	menaquinones	1190:1201	arg1	differentiating					1204:1218	differentiating	1204:1218	differentiating	1204:1218	Strain S5-TSA-19T had a distinctive chemotaxonomic pattern comprising A4α type peptidoglycan based on l-Lys-d-Asp, iso-C15 : 0 as the major fatty acid, absence of phosphatidylethanolamine as a major lipid and MK-7 and MK-6 as the major menaquinones, differentiating it from the genera Planococcus and Planomicrobium, thus supporting the findings of molecular phylogeny.
31287396	2	37	dep	pink-pigmented	101:114	arg1	coccoid-shaped					147:160	coccoid-shaped	147:160	coccoid-shaped	147:160	A pink-pigmented, Gram-stain-positive, aerobic, coccoid-shaped bacterial strain, designated as S5-TSA-19T, was isolated from an explosives contaminated site in Panchkula, Haryana, India.
31287396	2	37	dep	pink-pigmented	101:114	arg1	aerobic					138:144	aerobic	138:144	aerobic	138:144	A pink-pigmented, Gram-stain-positive, aerobic, coccoid-shaped bacterial strain, designated as S5-TSA-19T, was isolated from an explosives contaminated site in Panchkula, Haryana, India.
31287396	2	37	dep	pink-pigmented	101:114	arg1	Gram-stain-positive					117:135	Gram-stain-positive	117:135	Gram-stain-positive	117:135	A pink-pigmented, Gram-stain-positive, aerobic, coccoid-shaped bacterial strain, designated as S5-TSA-19T, was isolated from an explosives contaminated site in Panchkula, Haryana, India.
31287396	7	38	theme	Indiicoccus	1743:1753	arg1	nov.					1773:1776	Indiicoccus explosivorum gen. nov.	1743:1776	the name Indiicoccus explosivorum gen. nov.	1734:1776	On the basis of polyphasic taxonomic characterization and a phylogenomics approach, strain S5-TSA-19T is proposed as the type strain of a novel species in a novel genus for which the name Indiicoccus explosivorum gen. nov., sp.
31287396	6	39	contain	possess	1532:1538	arg1	strains					1516:1522	the closest reference strains	1494:1522	the closest reference strains	1494:1522	Further, strain S5-TSA-19T was able to biotransform hexahydro-1,3,5,-trinitro-1,2,5-triazine (RDX) into nitrite derivatives under aerobic conditions in 2-4 days, whereas the closest reference strains did not possess this property.
31287396	6	39	contain	possess	1532:1538	arg2	property					1545:1552	this property	1540:1552	this property	1540:1552	Further, strain S5-TSA-19T was able to biotransform hexahydro-1,3,5,-trinitro-1,2,5-triazine (RDX) into nitrite derivatives under aerobic conditions in 2-4 days, whereas the closest reference strains did not possess this property.
31287396	7	40	theme	species	1699:1705	arg1	S5-TSA-19T					1646:1655	strain S5-TSA-19T	1639:1655	strain S5-TSA-19T	1639:1655	On the basis of polyphasic taxonomic characterization and a phylogenomics approach, strain S5-TSA-19T is proposed as the type strain of a novel species in a novel genus for which the name Indiicoccus explosivorum gen. nov., sp.
31287396	7	40	theme	species	1699:1705	arg1	strain					1681:1686	the type strain	1672:1686	the type strain of a novel species in a novel genus for which the name Indiicoccus explosivorum gen. nov., sp	1672:1780	On the basis of polyphasic taxonomic characterization and a phylogenomics approach, strain S5-TSA-19T is proposed as the type strain of a novel species in a novel genus for which the name Indiicoccus explosivorum gen. nov., sp.
31287396	2	41	theme	pink-pigmented	101:114	arg1	strain					172:177	A pink-pigmented, Gram-stain-positive, aerobic, coccoid-shaped bacterial strain	99:177	A pink-pigmented, Gram-stain-positive, aerobic, coccoid-shaped bacterial strain	99:177	A pink-pigmented, Gram-stain-positive, aerobic, coccoid-shaped bacterial strain, designated as S5-TSA-19T, was isolated from an explosives contaminated site in Panchkula, Haryana, India.
31287396	4	42	theme	family	931:936	arg1	Planococcaceae					938:951	the family Planococcaceae	927:951	the family Planococcaceae	927:951	Phylogenetic analysis based on 16S rRNA gene and whole-genome sequences (based on a conserved set of 400 proteins) retrieved the strain in a distinct branch indicating a separate lineage within the family Planococcaceae.
31287396	5	43	theme	phylogeny	1313:1321	arg1	findings					1291:1298	the findings	1287:1298	the findings of molecular phylogeny	1287:1321	Strain S5-TSA-19T had a distinctive chemotaxonomic pattern comprising A4α type peptidoglycan based on l-Lys-d-Asp, iso-C15 : 0 as the major fatty acid, absence of phosphatidylethanolamine as a major lipid and MK-7 and MK-6 as the major menaquinones, differentiating it from the genera Planococcus and Planomicrobium, thus supporting the findings of molecular phylogeny.
31287396	5	44	theme	major	1088:1092	arg1	acid					1100:1103	the major fatty acid	1084:1103	the major fatty acid	1084:1103	Strain S5-TSA-19T had a distinctive chemotaxonomic pattern comprising A4α type peptidoglycan based on l-Lys-d-Asp, iso-C15 : 0 as the major fatty acid, absence of phosphatidylethanolamine as a major lipid and MK-7 and MK-6 as the major menaquinones, differentiating it from the genera Planococcus and Planomicrobium, thus supporting the findings of molecular phylogeny.
31287396	4	45	theme	16S	764:766	arg1	rRNA					768:771	16S rRNA	764:771	16S rRNA gene	764:776	Phylogenetic analysis based on 16S rRNA gene and whole-genome sequences (based on a conserved set of 400 proteins) retrieved the strain in a distinct branch indicating a separate lineage within the family Planococcaceae.
31287396	7	46	dep	sp	1779:1780	arg1	name					1738:1741	the name	1734:1741	the name Indiicoccus explosivorum gen. nov.	1734:1776	On the basis of polyphasic taxonomic characterization and a phylogenomics approach, strain S5-TSA-19T is proposed as the type strain of a novel species in a novel genus for which the name Indiicoccus explosivorum gen. nov., sp.
31287396	3	47	theme	Planococcus	485:495	arg1	S1T					509:511	Planococcus maitriensis S1T	485:511	Planococcus maitriensis S1T (95.6 %)	485:520	The 16S rRNA gene sequencing blast analysis indicated that the strain is a member of the family Planococcaceae with the highest sequence similarity to Planomicrobium soli XN13T (96.1 %), followed by Planococcus maitriensis S1T (95.6 %), Planococcus plakortidis DSM 23997T (95.6 %), Planomicrobium flavidum ISL-41T (95.6 %), Planococcus rifietoensis M8T (95.5 %), Planococcus salinus LCB217T (95.5 %) and Planococcus maritimus DSM 17275T (95.5 %).
31287396	3	47	theme	Planococcus	485:495	arg1	%					519:519	95.6 %	514:519	95.6 %	514:519	The 16S rRNA gene sequencing blast analysis indicated that the strain is a member of the family Planococcaceae with the highest sequence similarity to Planomicrobium soli XN13T (96.1 %), followed by Planococcus maitriensis S1T (95.6 %), Planococcus plakortidis DSM 23997T (95.6 %), Planomicrobium flavidum ISL-41T (95.6 %), Planococcus rifietoensis M8T (95.5 %), Planococcus salinus LCB217T (95.5 %) and Planococcus maritimus DSM 17275T (95.5 %).
31287396	6	48	theme	nitrite	1428:1434	arg1	derivatives					1436:1446	nitrite derivatives	1428:1446	nitrite derivatives	1428:1446	Further, strain S5-TSA-19T was able to biotransform hexahydro-1,3,5,-trinitro-1,2,5-triazine (RDX) into nitrite derivatives under aerobic conditions in 2-4 days, whereas the closest reference strains did not possess this property.
31287396	3	49	dep	Planococcus	523:533	arg1	plakortidis					535:545	plakortidis	535:545	plakortidis	535:545	The 16S rRNA gene sequencing blast analysis indicated that the strain is a member of the family Planococcaceae with the highest sequence similarity to Planomicrobium soli XN13T (96.1 %), followed by Planococcus maitriensis S1T (95.6 %), Planococcus plakortidis DSM 23997T (95.6 %), Planomicrobium flavidum ISL-41T (95.6 %), Planococcus rifietoensis M8T (95.5 %), Planococcus salinus LCB217T (95.5 %) and Planococcus maritimus DSM 17275T (95.5 %).
31287396	4	50	theme	Phylogenetic	733:744	arg1	analysis					746:753	Phylogenetic analysis	733:753	Phylogenetic analysis based on 16S rRNA gene and whole-genome sequences (based on a conserved set of 400 proteins)	733:846	Phylogenetic analysis based on 16S rRNA gene and whole-genome sequences (based on a conserved set of 400 proteins) retrieved the strain in a distinct branch indicating a separate lineage within the family Planococcaceae.
31287396	3	51	theme	blast	315:319	arg1	analysis					321:328	The 16S rRNA gene sequencing blast analysis	286:328	The 16S rRNA gene sequencing blast analysis	286:328	The 16S rRNA gene sequencing blast analysis indicated that the strain is a member of the family Planococcaceae with the highest sequence similarity to Planomicrobium soli XN13T (96.1 %), followed by Planococcus maitriensis S1T (95.6 %), Planococcus plakortidis DSM 23997T (95.6 %), Planomicrobium flavidum ISL-41T (95.6 %), Planococcus rifietoensis M8T (95.5 %), Planococcus salinus LCB217T (95.5 %) and Planococcus maritimus DSM 17275T (95.5 %).
31287396	3	52	with	member	361:366	arg1	similarity					423:432	the highest sequence similarity	402:432	the highest sequence similarity to Planomicrobium soli XN13T (96.1 %)	402:470	The 16S rRNA gene sequencing blast analysis indicated that the strain is a member of the family Planococcaceae with the highest sequence similarity to Planomicrobium soli XN13T (96.1 %), followed by Planococcus maitriensis S1T (95.6 %), Planococcus plakortidis DSM 23997T (95.6 %), Planomicrobium flavidum ISL-41T (95.6 %), Planococcus rifietoensis M8T (95.5 %), Planococcus salinus LCB217T (95.5 %) and Planococcus maritimus DSM 17275T (95.5 %).
31287396	3	53	theme	Planococcus	649:659	arg1	LCB217T					669:675	Planococcus salinus LCB217T	649:675	Planococcus salinus LCB217T (95.5 %)	649:684	The 16S rRNA gene sequencing blast analysis indicated that the strain is a member of the family Planococcaceae with the highest sequence similarity to Planomicrobium soli XN13T (96.1 %), followed by Planococcus maitriensis S1T (95.6 %), Planococcus plakortidis DSM 23997T (95.6 %), Planomicrobium flavidum ISL-41T (95.6 %), Planococcus rifietoensis M8T (95.5 %), Planococcus salinus LCB217T (95.5 %) and Planococcus maritimus DSM 17275T (95.5 %).
31287396	3	53	theme	Planococcus	649:659	arg1	%					683:683	95.5 %	678:683	95.5 %	678:683	The 16S rRNA gene sequencing blast analysis indicated that the strain is a member of the family Planococcaceae with the highest sequence similarity to Planomicrobium soli XN13T (96.1 %), followed by Planococcus maitriensis S1T (95.6 %), Planococcus plakortidis DSM 23997T (95.6 %), Planomicrobium flavidum ISL-41T (95.6 %), Planococcus rifietoensis M8T (95.5 %), Planococcus salinus LCB217T (95.5 %) and Planococcus maritimus DSM 17275T (95.5 %).
31287396	3	54	dep	Planococcus	690:700	arg1	maritimus					702:710	maritimus	702:710	maritimus	702:710	The 16S rRNA gene sequencing blast analysis indicated that the strain is a member of the family Planococcaceae with the highest sequence similarity to Planomicrobium soli XN13T (96.1 %), followed by Planococcus maitriensis S1T (95.6 %), Planococcus plakortidis DSM 23997T (95.6 %), Planomicrobium flavidum ISL-41T (95.6 %), Planococcus rifietoensis M8T (95.5 %), Planococcus salinus LCB217T (95.5 %) and Planococcus maritimus DSM 17275T (95.5 %).
31287396	3	55	theme	DSM	547:549	arg1	23997T					551:556	Planococcus plakortidis DSM 23997T	523:556	Planococcus plakortidis DSM 23997T (95.6 %)	523:565	The 16S rRNA gene sequencing blast analysis indicated that the strain is a member of the family Planococcaceae with the highest sequence similarity to Planomicrobium soli XN13T (96.1 %), followed by Planococcus maitriensis S1T (95.6 %), Planococcus plakortidis DSM 23997T (95.6 %), Planomicrobium flavidum ISL-41T (95.6 %), Planococcus rifietoensis M8T (95.5 %), Planococcus salinus LCB217T (95.5 %) and Planococcus maritimus DSM 17275T (95.5 %).
31287396	3	55	theme	DSM	547:549	arg1	%					564:564	95.6 %	559:564	95.6 %	559:564	The 16S rRNA gene sequencing blast analysis indicated that the strain is a member of the family Planococcaceae with the highest sequence similarity to Planomicrobium soli XN13T (96.1 %), followed by Planococcus maitriensis S1T (95.6 %), Planococcus plakortidis DSM 23997T (95.6 %), Planomicrobium flavidum ISL-41T (95.6 %), Planococcus rifietoensis M8T (95.5 %), Planococcus salinus LCB217T (95.5 %) and Planococcus maritimus DSM 17275T (95.5 %).
31287396	8	56	theme	31737T=KCTC	1806:1816	arg1	12608T					1830:1835	=JCM 31737T=KCTC 33871T=MTCC 12608T	1801:1835	=JCM 31737T=KCTC 33871T=MTCC 12608T	1801:1835	nov. is proposed (=JCM 31737T=KCTC 33871T=MTCC 12608T).
31287396	3	57	theme	Planococcus	523:533	arg1	23997T					551:556	Planococcus plakortidis DSM 23997T	523:556	Planococcus plakortidis DSM 23997T (95.6 %)	523:565	The 16S rRNA gene sequencing blast analysis indicated that the strain is a member of the family Planococcaceae with the highest sequence similarity to Planomicrobium soli XN13T (96.1 %), followed by Planococcus maitriensis S1T (95.6 %), Planococcus plakortidis DSM 23997T (95.6 %), Planomicrobium flavidum ISL-41T (95.6 %), Planococcus rifietoensis M8T (95.5 %), Planococcus salinus LCB217T (95.5 %) and Planococcus maritimus DSM 17275T (95.5 %).
31287396	3	57	theme	Planococcus	523:533	arg1	%					564:564	95.6 %	559:564	95.6 %	559:564	The 16S rRNA gene sequencing blast analysis indicated that the strain is a member of the family Planococcaceae with the highest sequence similarity to Planomicrobium soli XN13T (96.1 %), followed by Planococcus maitriensis S1T (95.6 %), Planococcus plakortidis DSM 23997T (95.6 %), Planomicrobium flavidum ISL-41T (95.6 %), Planococcus rifietoensis M8T (95.5 %), Planococcus salinus LCB217T (95.5 %) and Planococcus maritimus DSM 17275T (95.5 %).
31287396	3	58	dep	Planococcus	485:495	arg1	maitriensis					497:507	maitriensis	497:507	maitriensis	497:507	The 16S rRNA gene sequencing blast analysis indicated that the strain is a member of the family Planococcaceae with the highest sequence similarity to Planomicrobium soli XN13T (96.1 %), followed by Planococcus maitriensis S1T (95.6 %), Planococcus plakortidis DSM 23997T (95.6 %), Planomicrobium flavidum ISL-41T (95.6 %), Planococcus rifietoensis M8T (95.5 %), Planococcus salinus LCB217T (95.5 %) and Planococcus maritimus DSM 17275T (95.5 %).
31287396	3	59	theme	16S	290:292	arg1	analysis					321:328	The 16S rRNA gene sequencing blast analysis	286:328	The 16S rRNA gene sequencing blast analysis	286:328	The 16S rRNA gene sequencing blast analysis indicated that the strain is a member of the family Planococcaceae with the highest sequence similarity to Planomicrobium soli XN13T (96.1 %), followed by Planococcus maitriensis S1T (95.6 %), Planococcus plakortidis DSM 23997T (95.6 %), Planomicrobium flavidum ISL-41T (95.6 %), Planococcus rifietoensis M8T (95.5 %), Planococcus salinus LCB217T (95.5 %) and Planococcus maritimus DSM 17275T (95.5 %).
31287396	4	60	theme	whole-genome	782:793	arg1	sequences					795:803	whole-genome sequences	782:803	whole-genome sequences (based on a conserved set of 400 proteins)	782:846	Phylogenetic analysis based on 16S rRNA gene and whole-genome sequences (based on a conserved set of 400 proteins) retrieved the strain in a distinct branch indicating a separate lineage within the family Planococcaceae.
31287396	3	61	theme	DSM	712:714	arg1	17275T					716:721	Planococcus maritimus DSM 17275T	690:721	Planococcus maritimus DSM 17275T (95.5 %)	690:730	The 16S rRNA gene sequencing blast analysis indicated that the strain is a member of the family Planococcaceae with the highest sequence similarity to Planomicrobium soli XN13T (96.1 %), followed by Planococcus maitriensis S1T (95.6 %), Planococcus plakortidis DSM 23997T (95.6 %), Planomicrobium flavidum ISL-41T (95.6 %), Planococcus rifietoensis M8T (95.5 %), Planococcus salinus LCB217T (95.5 %) and Planococcus maritimus DSM 17275T (95.5 %).
31287396	3	61	theme	DSM	712:714	arg1	%					729:729	95.5 %	724:729	95.5 %	724:729	The 16S rRNA gene sequencing blast analysis indicated that the strain is a member of the family Planococcaceae with the highest sequence similarity to Planomicrobium soli XN13T (96.1 %), followed by Planococcus maitriensis S1T (95.6 %), Planococcus plakortidis DSM 23997T (95.6 %), Planomicrobium flavidum ISL-41T (95.6 %), Planococcus rifietoensis M8T (95.5 %), Planococcus salinus LCB217T (95.5 %) and Planococcus maritimus DSM 17275T (95.5 %).
31287396	2	62	theme	contaminated	238:249	arg1	site					251:254	an explosives contaminated site	224:254	an explosives contaminated site in Panchkula, Haryana, India	224:283	A pink-pigmented, Gram-stain-positive, aerobic, coccoid-shaped bacterial strain, designated as S5-TSA-19T, was isolated from an explosives contaminated site in Panchkula, Haryana, India.
31287396	7	63	theme	strain	1639:1644	arg1	S5-TSA-19T					1646:1655	strain S5-TSA-19T	1639:1655	strain S5-TSA-19T	1639:1655	On the basis of polyphasic taxonomic characterization and a phylogenomics approach, strain S5-TSA-19T is proposed as the type strain of a novel species in a novel genus for which the name Indiicoccus explosivorum gen. nov., sp.
31287396	7	63	theme	strain	1639:1644	arg1	strain					1681:1686	the type strain	1672:1686	the type strain of a novel species in a novel genus for which the name Indiicoccus explosivorum gen. nov., sp	1672:1780	On the basis of polyphasic taxonomic characterization and a phylogenomics approach, strain S5-TSA-19T is proposed as the type strain of a novel species in a novel genus for which the name Indiicoccus explosivorum gen. nov., sp.
31287396	4	64	theme	proteins	838:845	arg1	set					827:829	a conserved set	815:829	a conserved set of 400 proteins	815:845	Phylogenetic analysis based on 16S rRNA gene and whole-genome sequences (based on a conserved set of 400 proteins) retrieved the strain in a distinct branch indicating a separate lineage within the family Planococcaceae.
31287396	4	64	theme	proteins	838:845	arg1	proteins					838:845	400 proteins	834:845	400 proteins	834:845	Phylogenetic analysis based on 16S rRNA gene and whole-genome sequences (based on a conserved set of 400 proteins) retrieved the strain in a distinct branch indicating a separate lineage within the family Planococcaceae.
31287396	5	65	theme	phosphatidylethanolamine	1117:1140	arg1	iso-C15 					1069:1076	iso-C15 	1069:1076	iso-C15 	1069:1076	Strain S5-TSA-19T had a distinctive chemotaxonomic pattern comprising A4α type peptidoglycan based on l-Lys-d-Asp, iso-C15 : 0 as the major fatty acid, absence of phosphatidylethanolamine as a major lipid and MK-7 and MK-6 as the major menaquinones, differentiating it from the genera Planococcus and Planomicrobium, thus supporting the findings of molecular phylogeny.
31287396	5	65	theme	phosphatidylethanolamine	1117:1140	arg1	absence					1106:1112	absence	1106:1112	absence of phosphatidylethanolamine as a major lipid and MK-7 and MK-6 as the major menaquinones, differentiating it from the genera Planococcus and Planomicrobium, thus supporting the findings of molecular phylogeny	1106:1321	Strain S5-TSA-19T had a distinctive chemotaxonomic pattern comprising A4α type peptidoglycan based on l-Lys-d-Asp, iso-C15 : 0 as the major fatty acid, absence of phosphatidylethanolamine as a major lipid and MK-7 and MK-6 as the major menaquinones, differentiating it from the genera Planococcus and Planomicrobium, thus supporting the findings of molecular phylogeny.
31287396	3	66	theme	Planococcus	690:700	arg1	17275T					716:721	Planococcus maritimus DSM 17275T	690:721	Planococcus maritimus DSM 17275T (95.5 %)	690:730	The 16S rRNA gene sequencing blast analysis indicated that the strain is a member of the family Planococcaceae with the highest sequence similarity to Planomicrobium soli XN13T (96.1 %), followed by Planococcus maitriensis S1T (95.6 %), Planococcus plakortidis DSM 23997T (95.6 %), Planomicrobium flavidum ISL-41T (95.6 %), Planococcus rifietoensis M8T (95.5 %), Planococcus salinus LCB217T (95.5 %) and Planococcus maritimus DSM 17275T (95.5 %).
31287396	3	66	theme	Planococcus	690:700	arg1	%					729:729	95.5 %	724:729	95.5 %	724:729	The 16S rRNA gene sequencing blast analysis indicated that the strain is a member of the family Planococcaceae with the highest sequence similarity to Planomicrobium soli XN13T (96.1 %), followed by Planococcus maitriensis S1T (95.6 %), Planococcus plakortidis DSM 23997T (95.6 %), Planomicrobium flavidum ISL-41T (95.6 %), Planococcus rifietoensis M8T (95.5 %), Planococcus salinus LCB217T (95.5 %) and Planococcus maritimus DSM 17275T (95.5 %).
31287396	3	67	dep	Planococcus	649:659	arg1	salinus					661:667	salinus	661:667	salinus	661:667	The 16S rRNA gene sequencing blast analysis indicated that the strain is a member of the family Planococcaceae with the highest sequence similarity to Planomicrobium soli XN13T (96.1 %), followed by Planococcus maitriensis S1T (95.6 %), Planococcus plakortidis DSM 23997T (95.6 %), Planomicrobium flavidum ISL-41T (95.6 %), Planococcus rifietoensis M8T (95.5 %), Planococcus salinus LCB217T (95.5 %) and Planococcus maritimus DSM 17275T (95.5 %).
31287396	3	68	theme	gene	299:302	arg1	analysis					321:328	The 16S rRNA gene sequencing blast analysis	286:328	The 16S rRNA gene sequencing blast analysis	286:328	The 16S rRNA gene sequencing blast analysis indicated that the strain is a member of the family Planococcaceae with the highest sequence similarity to Planomicrobium soli XN13T (96.1 %), followed by Planococcus maitriensis S1T (95.6 %), Planococcus plakortidis DSM 23997T (95.6 %), Planomicrobium flavidum ISL-41T (95.6 %), Planococcus rifietoensis M8T (95.5 %), Planococcus salinus LCB217T (95.5 %) and Planococcus maritimus DSM 17275T (95.5 %).
31287396	5	69	theme	type	1028:1031	arg1	peptidoglycan					1033:1045	A4α type peptidoglycan	1024:1045	A4α type peptidoglycan	1024:1045	Strain S5-TSA-19T had a distinctive chemotaxonomic pattern comprising A4α type peptidoglycan based on l-Lys-d-Asp, iso-C15 : 0 as the major fatty acid, absence of phosphatidylethanolamine as a major lipid and MK-7 and MK-6 as the major menaquinones, differentiating it from the genera Planococcus and Planomicrobium, thus supporting the findings of molecular phylogeny.
31287396	4	70	theme	conserved	817:825	arg1	set					827:829	a conserved set	815:829	a conserved set of 400 proteins	815:845	Phylogenetic analysis based on 16S rRNA gene and whole-genome sequences (based on a conserved set of 400 proteins) retrieved the strain in a distinct branch indicating a separate lineage within the family Planococcaceae.
31287396	4	70	theme	conserved	817:825	arg1	proteins					838:845	400 proteins	834:845	400 proteins	834:845	Phylogenetic analysis based on 16S rRNA gene and whole-genome sequences (based on a conserved set of 400 proteins) retrieved the strain in a distinct branch indicating a separate lineage within the family Planococcaceae.
31287396	7	71	from	species	1699:1705	arg1	genus					1718:1722	a novel genus	1710:1722	a novel genus for which the name Indiicoccus explosivorum gen. nov., sp	1710:1780	On the basis of polyphasic taxonomic characterization and a phylogenomics approach, strain S5-TSA-19T is proposed as the type strain of a novel species in a novel genus for which the name Indiicoccus explosivorum gen. nov., sp.
31287396	5	72	contain	had	972:974	arg2	pattern					1005:1011	a distinctive chemotaxonomic pattern	976:1011	a distinctive chemotaxonomic pattern comprising A4α type peptidoglycan	976:1045	Strain S5-TSA-19T had a distinctive chemotaxonomic pattern comprising A4α type peptidoglycan based on l-Lys-d-Asp, iso-C15 : 0 as the major fatty acid, absence of phosphatidylethanolamine as a major lipid and MK-7 and MK-6 as the major menaquinones, differentiating it from the genera Planococcus and Planomicrobium, thus supporting the findings of molecular phylogeny.
31287396	5	72	contain	had	972:974	arg1	S5-TSA-19T					961:970	Strain S5-TSA-19T	954:970	Strain S5-TSA-19T	954:970	Strain S5-TSA-19T had a distinctive chemotaxonomic pattern comprising A4α type peptidoglycan based on l-Lys-d-Asp, iso-C15 : 0 as the major fatty acid, absence of phosphatidylethanolamine as a major lipid and MK-7 and MK-6 as the major menaquinones, differentiating it from the genera Planococcus and Planomicrobium, thus supporting the findings of molecular phylogeny.
31287396	3	73	theme	sequence	414:421	arg1	similarity					423:432	the highest sequence similarity	402:432	the highest sequence similarity to Planomicrobium soli XN13T (96.1 %)	402:470	The 16S rRNA gene sequencing blast analysis indicated that the strain is a member of the family Planococcaceae with the highest sequence similarity to Planomicrobium soli XN13T (96.1 %), followed by Planococcus maitriensis S1T (95.6 %), Planococcus plakortidis DSM 23997T (95.6 %), Planomicrobium flavidum ISL-41T (95.6 %), Planococcus rifietoensis M8T (95.5 %), Planococcus salinus LCB217T (95.5 %) and Planococcus maritimus DSM 17275T (95.5 %).
31287396	0	74	theme	explosivorum	12:23	arg1	nov.					30:33	Indiicoccus explosivorum gen. nov.	0:33	Indiicoccus explosivorum gen. nov.	0:33	Indiicoccus explosivorum gen. nov., sp.
31287396	5	75	theme	chemotaxonomic	990:1003	arg1	pattern					1005:1011	a distinctive chemotaxonomic pattern	976:1011	a distinctive chemotaxonomic pattern comprising A4α type peptidoglycan	976:1045	Strain S5-TSA-19T had a distinctive chemotaxonomic pattern comprising A4α type peptidoglycan based on l-Lys-d-Asp, iso-C15 : 0 as the major fatty acid, absence of phosphatidylethanolamine as a major lipid and MK-7 and MK-6 as the major menaquinones, differentiating it from the genera Planococcus and Planomicrobium, thus supporting the findings of molecular phylogeny.
31287396	1	76	theme	explosives	63:72	arg1	site					93:96	an explosives waste contaminated site	60:96	an explosives waste contaminated site	60:96	nov., isolated from an explosives waste contaminated site.
31287396	7	77	theme	polyphasic	1571:1580	arg1	characterization					1592:1607	polyphasic taxonomic characterization	1571:1607	polyphasic taxonomic characterization	1571:1607	On the basis of polyphasic taxonomic characterization and a phylogenomics approach, strain S5-TSA-19T is proposed as the type strain of a novel species in a novel genus for which the name Indiicoccus explosivorum gen. nov., sp.
31287396	6	78	theme	closest	1498:1504	arg1	strains					1516:1522	the closest reference strains	1494:1522	the closest reference strains	1494:1522	Further, strain S5-TSA-19T was able to biotransform hexahydro-1,3,5,-trinitro-1,2,5-triazine (RDX) into nitrite derivatives under aerobic conditions in 2-4 days, whereas the closest reference strains did not possess this property.
31287396	1	79	theme	contaminated	80:91	arg1	site					93:96	an explosives waste contaminated site	60:96	an explosives waste contaminated site	60:96	nov., isolated from an explosives waste contaminated site.
31287396	7	80	from	genus	1718:1722	arg1	S5-TSA-19T					1646:1655	strain S5-TSA-19T	1639:1655	strain S5-TSA-19T	1639:1655	On the basis of polyphasic taxonomic characterization and a phylogenomics approach, strain S5-TSA-19T is proposed as the type strain of a novel species in a novel genus for which the name Indiicoccus explosivorum gen. nov., sp.
31287396	7	80	from	genus	1718:1722	arg1	strain					1681:1686	the type strain	1672:1686	the type strain of a novel species in a novel genus for which the name Indiicoccus explosivorum gen. nov., sp	1672:1780	On the basis of polyphasic taxonomic characterization and a phylogenomics approach, strain S5-TSA-19T is proposed as the type strain of a novel species in a novel genus for which the name Indiicoccus explosivorum gen. nov., sp.
31287396	3	81	theme	soli	452:455	arg1	XN13T					457:461	Planomicrobium soli XN13T	437:461	Planomicrobium soli XN13T (96.1 %)	437:470	The 16S rRNA gene sequencing blast analysis indicated that the strain is a member of the family Planococcaceae with the highest sequence similarity to Planomicrobium soli XN13T (96.1 %), followed by Planococcus maitriensis S1T (95.6 %), Planococcus plakortidis DSM 23997T (95.6 %), Planomicrobium flavidum ISL-41T (95.6 %), Planococcus rifietoensis M8T (95.5 %), Planococcus salinus LCB217T (95.5 %) and Planococcus maritimus DSM 17275T (95.5 %).
31287396	3	81	theme	soli	452:455	arg1	%					469:469	96.1 %	464:469	96.1 %	464:469	The 16S rRNA gene sequencing blast analysis indicated that the strain is a member of the family Planococcaceae with the highest sequence similarity to Planomicrobium soli XN13T (96.1 %), followed by Planococcus maitriensis S1T (95.6 %), Planococcus plakortidis DSM 23997T (95.6 %), Planomicrobium flavidum ISL-41T (95.6 %), Planococcus rifietoensis M8T (95.5 %), Planococcus salinus LCB217T (95.5 %) and Planococcus maritimus DSM 17275T (95.5 %).
31287396	2	82	from	site	251:254	arg1	Haryana					270:276	Haryana	270:276	Haryana	270:276	A pink-pigmented, Gram-stain-positive, aerobic, coccoid-shaped bacterial strain, designated as S5-TSA-19T, was isolated from an explosives contaminated site in Panchkula, Haryana, India.
31287396	2	82	from	site	251:254	arg1	Panchkula					259:267	Panchkula	259:267	Panchkula	259:267	A pink-pigmented, Gram-stain-positive, aerobic, coccoid-shaped bacterial strain, designated as S5-TSA-19T, was isolated from an explosives contaminated site in Panchkula, Haryana, India.
31287396	2	82	from	site	251:254	arg1	India					279:283	India	279:283	India	279:283	A pink-pigmented, Gram-stain-positive, aerobic, coccoid-shaped bacterial strain, designated as S5-TSA-19T, was isolated from an explosives contaminated site in Panchkula, Haryana, India.
31287396	3	83	theme	Planococcaceae	382:395	arg1	strain					349:354	the strain	345:354	the strain	345:354	The 16S rRNA gene sequencing blast analysis indicated that the strain is a member of the family Planococcaceae with the highest sequence similarity to Planomicrobium soli XN13T (96.1 %), followed by Planococcus maitriensis S1T (95.6 %), Planococcus plakortidis DSM 23997T (95.6 %), Planomicrobium flavidum ISL-41T (95.6 %), Planococcus rifietoensis M8T (95.5 %), Planococcus salinus LCB217T (95.5 %) and Planococcus maritimus DSM 17275T (95.5 %).
31287396	3	83	theme	Planococcaceae	382:395	arg1	member					361:366	a member	359:366	a member of the family Planococcaceae	359:395	The 16S rRNA gene sequencing blast analysis indicated that the strain is a member of the family Planococcaceae with the highest sequence similarity to Planomicrobium soli XN13T (96.1 %), followed by Planococcus maitriensis S1T (95.6 %), Planococcus plakortidis DSM 23997T (95.6 %), Planomicrobium flavidum ISL-41T (95.6 %), Planococcus rifietoensis M8T (95.5 %), Planococcus salinus LCB217T (95.5 %) and Planococcus maritimus DSM 17275T (95.5 %).
31287396	2	84	theme	bacterial	162:170	arg1	strain					172:177	A pink-pigmented, Gram-stain-positive, aerobic, coccoid-shaped bacterial strain	99:177	A pink-pigmented, Gram-stain-positive, aerobic, coccoid-shaped bacterial strain	99:177	A pink-pigmented, Gram-stain-positive, aerobic, coccoid-shaped bacterial strain, designated as S5-TSA-19T, was isolated from an explosives contaminated site in Panchkula, Haryana, India.
31287396	7	85	theme	explosivorum	1755:1766	arg1	nov.					1773:1776	Indiicoccus explosivorum gen. nov.	1743:1776	the name Indiicoccus explosivorum gen. nov.	1734:1776	On the basis of polyphasic taxonomic characterization and a phylogenomics approach, strain S5-TSA-19T is proposed as the type strain of a novel species in a novel genus for which the name Indiicoccus explosivorum gen. nov., sp.
31287396	0	86	dep	sp	36:37	arg1	nov.					30:33	Indiicoccus explosivorum gen. nov.	0:33	Indiicoccus explosivorum gen. nov.	0:33	Indiicoccus explosivorum gen. nov., sp.
31287396	4	87	from	strain	862:867	arg1	branch					883:888	a distinct branch	872:888	a distinct branch	872:888	Phylogenetic analysis based on 16S rRNA gene and whole-genome sequences (based on a conserved set of 400 proteins) retrieved the strain in a distinct branch indicating a separate lineage within the family Planococcaceae.
31287396	3	88	dep	Planomicrobium	568:581	arg1	flavidum					583:590	flavidum	583:590	flavidum	583:590	The 16S rRNA gene sequencing blast analysis indicated that the strain is a member of the family Planococcaceae with the highest sequence similarity to Planomicrobium soli XN13T (96.1 %), followed by Planococcus maitriensis S1T (95.6 %), Planococcus plakortidis DSM 23997T (95.6 %), Planomicrobium flavidum ISL-41T (95.6 %), Planococcus rifietoensis M8T (95.5 %), Planococcus salinus LCB217T (95.5 %) and Planococcus maritimus DSM 17275T (95.5 %).
31287396	7	89	theme	novel	1712:1716	arg1	genus					1718:1722	a novel genus	1710:1722	a novel genus for which the name Indiicoccus explosivorum gen. nov., sp	1710:1780	On the basis of polyphasic taxonomic characterization and a phylogenomics approach, strain S5-TSA-19T is proposed as the type strain of a novel species in a novel genus for which the name Indiicoccus explosivorum gen. nov., sp.
31287396	7	90	from	strain	1681:1686	arg1	genus					1718:1722	a novel genus	1710:1722	a novel genus for which the name Indiicoccus explosivorum gen. nov., sp	1710:1780	On the basis of polyphasic taxonomic characterization and a phylogenomics approach, strain S5-TSA-19T is proposed as the type strain of a novel species in a novel genus for which the name Indiicoccus explosivorum gen. nov., sp.
31287396	5	91	theme	genera	1232:1237	arg1	Planococcus					1239:1249	the genera Planococcus	1228:1249	the genera Planococcus	1228:1249	Strain S5-TSA-19T had a distinctive chemotaxonomic pattern comprising A4α type peptidoglycan based on l-Lys-d-Asp, iso-C15 : 0 as the major fatty acid, absence of phosphatidylethanolamine as a major lipid and MK-7 and MK-6 as the major menaquinones, differentiating it from the genera Planococcus and Planomicrobium, thus supporting the findings of molecular phylogeny.
30022499	4	0	theme	soluble	538:544	arg1	NaCl					596:599	NaCl	596:599	NaCl	596:599	PB verified soluble starch (SS), colloidal chitosan (CC) peptone, and NaCl as most significant variables affecting chitosanase production.
30022499	4	0	theme	soluble	538:544	arg1	starch					546:551	soluble starch	538:551	soluble starch (SS)	538:556	PB verified soluble starch (SS), colloidal chitosan (CC) peptone, and NaCl as most significant variables affecting chitosanase production.
30022499	4	0	theme	soluble	538:544	arg1	peptone					583:589	colloidal chitosan (CC) peptone	559:589	colloidal chitosan (CC) peptone	559:589	PB verified soluble starch (SS), colloidal chitosan (CC) peptone, and NaCl as most significant variables affecting chitosanase production.
30022499	4	0	theme	soluble	538:544	arg1	variables					621:629	most significant variables	604:629	most significant variables affecting chitosanase production	604:662	PB verified soluble starch (SS), colloidal chitosan (CC) peptone, and NaCl as most significant variables affecting chitosanase production.
30022499	4	0	theme	soluble	538:544	arg1	SS					554:555	SS	554:555	SS	554:555	PB verified soluble starch (SS), colloidal chitosan (CC) peptone, and NaCl as most significant variables affecting chitosanase production.
30022499	7	1	theme	18.6 U ml-1	962:972	arg1	activity					986:993	18.6 U ml-1 chitosanase activity	962:993	18.6 U ml-1 chitosanase activity	962:993	Lastly, 18.6 U ml-1 chitosanase activity was achieved in stirred tank bioreactor using optimal medium, which is quite satisfactory to proclaim this strain as a potential candidate to provide commercial chitosanase.
30022499	1	2	theme	indigenous	276:285	arg1	strain					287:292	an indigenous strain	273:292	an indigenous strain identified as Bacillus mojavensis	273:326	The present study was conducted to improve chitosanase activity of an indigenous strain identified as Bacillus mojavensis.
30022499	6	3	theme	chitosanase	897:907	arg1	production					909:918	chitosanase production	897:918	chitosanase production	897:918	Discovery of the novel optimal medium has improved chitosanase production by B. mojavensis up-to 9.5 folds.
30022499	2	4	theme	one-variable-at-a-time	386:407	arg1	approach					409:416	one-variable-at-a-time approach	386:416	one-variable-at-a-time approach	386:416	Initially, carbon and nitrogen sources were optimized by one-variable-at-a-time approach.
30022499	1	5	theme	strain	287:292	arg1	activity					261:268	chitosanase activity	249:268	chitosanase activity of an indigenous strain identified as Bacillus mojavensis	249:326	The present study was conducted to improve chitosanase activity of an indigenous strain identified as Bacillus mojavensis.
30022499	0	6	theme	enzymes	119:125	arg1	production					82:91	Bacillus mojavensis EGE-B-5.2i. Maximum production	42:91	Bacillus mojavensis EGE-B-5.2i. Maximum production of industrially important enzymes such as chitosanases through media optimization	42:173	Optimization of chitosanase production by Bacillus mojavensis EGE-B-5.2i. Maximum production of industrially important enzymes such as chitosanases through media optimization still holds foremost interest.
30022499	0	7	theme	important	109:117	arg1	enzymes					119:125	industrially important enzymes	96:125	industrially important enzymes such as chitosanases	96:146	Optimization of chitosanase production by Bacillus mojavensis EGE-B-5.2i. Maximum production of industrially important enzymes such as chitosanases through media optimization still holds foremost interest.
30022499	0	7	theme	important	109:117	arg1	chitosanases					135:146	chitosanases	135:146	chitosanases	135:146	Optimization of chitosanase production by Bacillus mojavensis EGE-B-5.2i. Maximum production of industrially important enzymes such as chitosanases through media optimization still holds foremost interest.
30022499	6	8	theme	medium	877:882	arg1	Discovery					846:854	Discovery	846:854	Discovery of the novel optimal medium	846:882	Discovery of the novel optimal medium has improved chitosanase production by B. mojavensis up-to 9.5 folds.
30022499	5	9	theme	optimum	691:697	arg1	concentrations					699:712	the optimum concentrations	687:712	the optimum concentrations of SS, CC, peptone, and NaCl	687:741	CCD results predicted the optimum concentrations of SS, CC, peptone, and NaCl as 7.8, 7.0, 6.5, and 2.7 g L-1 , respectively to achieve maximum chitosanase activity (21.1 U ml-1 ).
30022499	6	10	theme	B.	923:924	arg1	up-to					937:941	B. mojavensis up-to 9.5 folds	923:951	B. mojavensis up-to 9.5 folds	923:951	Discovery of the novel optimal medium has improved chitosanase production by B. mojavensis up-to 9.5 folds.
30022499	6	11	theme	optimal	869:875	arg1	medium					877:882	the novel optimal medium	859:882	the novel optimal medium	859:882	Discovery of the novel optimal medium has improved chitosanase production by B. mojavensis up-to 9.5 folds.
30022499	7	12	theme	potential	1114:1122	arg1	strain					1102:1107	this strain	1097:1107	this strain	1097:1107	Lastly, 18.6 U ml-1 chitosanase activity was achieved in stirred tank bioreactor using optimal medium, which is quite satisfactory to proclaim this strain as a potential candidate to provide commercial chitosanase.
30022499	7	12	theme	potential	1114:1122	arg1	candidate					1124:1132	a potential candidate	1112:1132	a potential candidate to provide commercial chitosanase	1112:1166	Lastly, 18.6 U ml-1 chitosanase activity was achieved in stirred tank bioreactor using optimal medium, which is quite satisfactory to proclaim this strain as a potential candidate to provide commercial chitosanase.
30022499	6	13	theme	novel	863:867	arg1	medium					877:882	the novel optimal medium	859:882	the novel optimal medium	859:882	Discovery of the novel optimal medium has improved chitosanase production by B. mojavensis up-to 9.5 folds.
30022499	3	14	theme	central	493:499	arg1	designs					511:517	central composite designs	493:517	central composite designs (CCD)	493:523	Further, fermentation medium was optimized using Plackett-Burman (PB) and central composite designs (CCD).
30022499	3	14	theme	central	493:499	arg1	CCD					520:522	CCD	520:522	CCD	520:522	Further, fermentation medium was optimized using Plackett-Burman (PB) and central composite designs (CCD).
30022499	2	15	theme	nitrogen	351:358	arg1	sources					360:366	carbon and nitrogen sources	340:366	carbon and nitrogen sources	340:366	Initially, carbon and nitrogen sources were optimized by one-variable-at-a-time approach.
30022499	1	16	theme	present	210:216	arg1	study					218:222	The present study	206:222	The present study	206:222	The present study was conducted to improve chitosanase activity of an indigenous strain identified as Bacillus mojavensis.
30022499	5	17	theme	CCD	665:667	arg1	results					669:675	CCD results	665:675	CCD results	665:675	CCD results predicted the optimum concentrations of SS, CC, peptone, and NaCl as 7.8, 7.0, 6.5, and 2.7 g L-1 , respectively to achieve maximum chitosanase activity (21.1 U ml-1 ).
30022499	0	18	theme	production	28:37	arg1	Optimization					0:11	Optimization	0:11	Optimization of chitosanase production by Bacillus mojavensis EGE-B-5.2i. Maximum production of industrially important enzymes such as chitosanases through media optimization	0:173	Optimization of chitosanase production by Bacillus mojavensis EGE-B-5.2i. Maximum production of industrially important enzymes such as chitosanases through media optimization still holds foremost interest.
30022499	5	19	theme	CC	721:722	arg1	concentrations					699:712	the optimum concentrations	687:712	the optimum concentrations of SS, CC, peptone, and NaCl	687:741	CCD results predicted the optimum concentrations of SS, CC, peptone, and NaCl as 7.8, 7.0, 6.5, and 2.7 g L-1 , respectively to achieve maximum chitosanase activity (21.1 U ml-1 ).
30022499	2	20	theme	carbon	340:345	arg1	sources					360:366	carbon and nitrogen sources	340:366	carbon and nitrogen sources	340:366	Initially, carbon and nitrogen sources were optimized by one-variable-at-a-time approach.
30022499	5	21	theme	chitosanase	809:819	arg1	21.1 U ml-1					831:841	21.1 U ml-1	831:841	21.1 U ml-1	831:841	CCD results predicted the optimum concentrations of SS, CC, peptone, and NaCl as 7.8, 7.0, 6.5, and 2.7 g L-1 , respectively to achieve maximum chitosanase activity (21.1 U ml-1 ).
30022499	5	21	theme	chitosanase	809:819	arg1	activity					821:828	maximum chitosanase activity	801:828	maximum chitosanase activity (21.1 U ml-1 )	801:843	CCD results predicted the optimum concentrations of SS, CC, peptone, and NaCl as 7.8, 7.0, 6.5, and 2.7 g L-1 , respectively to achieve maximum chitosanase activity (21.1 U ml-1 ).
30022499	3	22	theme	fermentation	428:439	arg1	medium					441:446	fermentation medium	428:446	fermentation medium	428:446	Further, fermentation medium was optimized using Plackett-Burman (PB) and central composite designs (CCD).
30022499	4	23	theme	chitosanase	641:651	arg1	production					653:662	chitosanase production	641:662	chitosanase production	641:662	PB verified soluble starch (SS), colloidal chitosan (CC) peptone, and NaCl as most significant variables affecting chitosanase production.
30022499	0	24	theme	Bacillus	42:49	arg1	production					82:91	Bacillus mojavensis EGE-B-5.2i. Maximum production	42:91	Bacillus mojavensis EGE-B-5.2i. Maximum production of industrially important enzymes such as chitosanases through media optimization	42:173	Optimization of chitosanase production by Bacillus mojavensis EGE-B-5.2i. Maximum production of industrially important enzymes such as chitosanases through media optimization still holds foremost interest.
30022499	5	25	theme	peptone	725:731	arg1	concentrations					699:712	the optimum concentrations	687:712	the optimum concentrations of SS, CC, peptone, and NaCl	687:741	CCD results predicted the optimum concentrations of SS, CC, peptone, and NaCl as 7.8, 7.0, 6.5, and 2.7 g L-1 , respectively to achieve maximum chitosanase activity (21.1 U ml-1 ).
30022499	3	26	theme	composite	501:509	arg1	designs					511:517	central composite designs	493:517	central composite designs (CCD)	493:523	Further, fermentation medium was optimized using Plackett-Burman (PB) and central composite designs (CCD).
30022499	3	26	theme	composite	501:509	arg1	CCD					520:522	CCD	520:522	CCD	520:522	Further, fermentation medium was optimized using Plackett-Burman (PB) and central composite designs (CCD).
30022499	0	27	theme	media	156:160	arg1	optimization					162:173	media optimization	156:173	media optimization	156:173	Optimization of chitosanase production by Bacillus mojavensis EGE-B-5.2i. Maximum production of industrially important enzymes such as chitosanases through media optimization still holds foremost interest.
30022499	6	28	dep	up-to	937:941	arg1	folds					947:951	folds	947:951	folds	947:951	Discovery of the novel optimal medium has improved chitosanase production by B. mojavensis up-to 9.5 folds.
30022499	7	29	theme	commercial	1145:1154	arg1	chitosanase					1156:1166	commercial chitosanase	1145:1166	commercial chitosanase	1145:1166	Lastly, 18.6 U ml-1 chitosanase activity was achieved in stirred tank bioreactor using optimal medium, which is quite satisfactory to proclaim this strain as a potential candidate to provide commercial chitosanase.
30022499	0	30	theme	EGE-B-5.2i.	62:72	arg1	production					82:91	Bacillus mojavensis EGE-B-5.2i. Maximum production	42:91	Bacillus mojavensis EGE-B-5.2i. Maximum production of industrially important enzymes such as chitosanases through media optimization	42:173	Optimization of chitosanase production by Bacillus mojavensis EGE-B-5.2i. Maximum production of industrially important enzymes such as chitosanases through media optimization still holds foremost interest.
30022499	6	31	theme	mojavensis	926:935	arg1	up-to					937:941	B. mojavensis up-to 9.5 folds	923:951	B. mojavensis up-to 9.5 folds	923:951	Discovery of the novel optimal medium has improved chitosanase production by B. mojavensis up-to 9.5 folds.
30022499	0	32	dep	Bacillus	42:49	arg1	mojavensis					51:60	mojavensis	51:60	mojavensis	51:60	Optimization of chitosanase production by Bacillus mojavensis EGE-B-5.2i. Maximum production of industrially important enzymes such as chitosanases through media optimization still holds foremost interest.
30022499	7	33	theme	tank	1019:1022	arg1	bioreactor					1024:1033	stirred tank bioreactor	1011:1033	stirred tank bioreactor using optimal medium, which is quite satisfactory to proclaim this strain as a potential candidate to provide commercial chitosanase	1011:1166	Lastly, 18.6 U ml-1 chitosanase activity was achieved in stirred tank bioreactor using optimal medium, which is quite satisfactory to proclaim this strain as a potential candidate to provide commercial chitosanase.
30022499	4	34	theme	chitosan	569:576	arg1	NaCl					596:599	NaCl	596:599	NaCl	596:599	PB verified soluble starch (SS), colloidal chitosan (CC) peptone, and NaCl as most significant variables affecting chitosanase production.
30022499	4	34	theme	chitosan	569:576	arg1	starch					546:551	soluble starch	538:551	soluble starch (SS)	538:556	PB verified soluble starch (SS), colloidal chitosan (CC) peptone, and NaCl as most significant variables affecting chitosanase production.
30022499	4	34	theme	chitosan	569:576	arg1	variables					621:629	most significant variables	604:629	most significant variables affecting chitosanase production	604:662	PB verified soluble starch (SS), colloidal chitosan (CC) peptone, and NaCl as most significant variables affecting chitosanase production.
30022499	4	34	theme	chitosan	569:576	arg1	peptone					583:589	colloidal chitosan (CC) peptone	559:589	colloidal chitosan (CC) peptone	559:589	PB verified soluble starch (SS), colloidal chitosan (CC) peptone, and NaCl as most significant variables affecting chitosanase production.
30022499	7	35	theme	optimal	1041:1047	arg1	medium					1049:1054	optimal medium	1041:1054	optimal medium	1041:1054	Lastly, 18.6 U ml-1 chitosanase activity was achieved in stirred tank bioreactor using optimal medium, which is quite satisfactory to proclaim this strain as a potential candidate to provide commercial chitosanase.
30022499	4	36	theme	colloidal	559:567	arg1	CC					579:580	CC	579:580	CC	579:580	PB verified soluble starch (SS), colloidal chitosan (CC) peptone, and NaCl as most significant variables affecting chitosanase production.
30022499	4	36	theme	colloidal	559:567	arg1	chitosan					569:576	colloidal chitosan	559:576	colloidal chitosan (CC) peptone	559:589	PB verified soluble starch (SS), colloidal chitosan (CC) peptone, and NaCl as most significant variables affecting chitosanase production.
30022499	5	37	theme	SS	717:718	arg1	concentrations					699:712	the optimum concentrations	687:712	the optimum concentrations of SS, CC, peptone, and NaCl	687:741	CCD results predicted the optimum concentrations of SS, CC, peptone, and NaCl as 7.8, 7.0, 6.5, and 2.7 g L-1 , respectively to achieve maximum chitosanase activity (21.1 U ml-1 ).
30022499	7	38	theme	stirred	1011:1017	arg1	bioreactor					1024:1033	stirred tank bioreactor	1011:1033	stirred tank bioreactor using optimal medium, which is quite satisfactory to proclaim this strain as a potential candidate to provide commercial chitosanase	1011:1166	Lastly, 18.6 U ml-1 chitosanase activity was achieved in stirred tank bioreactor using optimal medium, which is quite satisfactory to proclaim this strain as a potential candidate to provide commercial chitosanase.
30022499	1	39	theme	chitosanase	249:259	arg1	activity					261:268	chitosanase activity	249:268	chitosanase activity of an indigenous strain identified as Bacillus mojavensis	249:326	The present study was conducted to improve chitosanase activity of an indigenous strain identified as Bacillus mojavensis.
30022499	0	40	theme	foremost	187:194	arg1	interest					196:203	foremost interest	187:203	foremost interest	187:203	Optimization of chitosanase production by Bacillus mojavensis EGE-B-5.2i. Maximum production of industrially important enzymes such as chitosanases through media optimization still holds foremost interest.
30022499	5	41	theme	maximum	801:807	arg1	21.1 U ml-1					831:841	21.1 U ml-1	831:841	21.1 U ml-1	831:841	CCD results predicted the optimum concentrations of SS, CC, peptone, and NaCl as 7.8, 7.0, 6.5, and 2.7 g L-1 , respectively to achieve maximum chitosanase activity (21.1 U ml-1 ).
30022499	5	41	theme	maximum	801:807	arg1	activity					821:828	maximum chitosanase activity	801:828	maximum chitosanase activity (21.1 U ml-1 )	801:843	CCD results predicted the optimum concentrations of SS, CC, peptone, and NaCl as 7.8, 7.0, 6.5, and 2.7 g L-1 , respectively to achieve maximum chitosanase activity (21.1 U ml-1 ).
30022499	7	42	theme	chitosanase	974:984	arg1	activity					986:993	18.6 U ml-1 chitosanase activity	962:993	18.6 U ml-1 chitosanase activity	962:993	Lastly, 18.6 U ml-1 chitosanase activity was achieved in stirred tank bioreactor using optimal medium, which is quite satisfactory to proclaim this strain as a potential candidate to provide commercial chitosanase.
30022499	4	43	theme	most	604:607	arg1	NaCl					596:599	NaCl	596:599	NaCl	596:599	PB verified soluble starch (SS), colloidal chitosan (CC) peptone, and NaCl as most significant variables affecting chitosanase production.
30022499	4	43	theme	most	604:607	arg1	starch					546:551	soluble starch	538:551	soluble starch (SS)	538:556	PB verified soluble starch (SS), colloidal chitosan (CC) peptone, and NaCl as most significant variables affecting chitosanase production.
30022499	4	43	theme	most	604:607	arg1	variables					621:629	most significant variables	604:629	most significant variables affecting chitosanase production	604:662	PB verified soluble starch (SS), colloidal chitosan (CC) peptone, and NaCl as most significant variables affecting chitosanase production.
30022499	4	43	theme	most	604:607	arg1	peptone					583:589	colloidal chitosan (CC) peptone	559:589	colloidal chitosan (CC) peptone	559:589	PB verified soluble starch (SS), colloidal chitosan (CC) peptone, and NaCl as most significant variables affecting chitosanase production.
30022499	0	44	theme	Maximum	74:80	arg1	production					82:91	Bacillus mojavensis EGE-B-5.2i. Maximum production	42:91	Bacillus mojavensis EGE-B-5.2i. Maximum production of industrially important enzymes such as chitosanases through media optimization	42:173	Optimization of chitosanase production by Bacillus mojavensis EGE-B-5.2i. Maximum production of industrially important enzymes such as chitosanases through media optimization still holds foremost interest.
30022499	0	45	theme	chitosanase	16:26	arg1	production					28:37	chitosanase production	16:37	chitosanase production	16:37	Optimization of chitosanase production by Bacillus mojavensis EGE-B-5.2i. Maximum production of industrially important enzymes such as chitosanases through media optimization still holds foremost interest.
30022499	4	46	theme	significant	609:619	arg1	NaCl					596:599	NaCl	596:599	NaCl	596:599	PB verified soluble starch (SS), colloidal chitosan (CC) peptone, and NaCl as most significant variables affecting chitosanase production.
30022499	4	46	theme	significant	609:619	arg1	starch					546:551	soluble starch	538:551	soluble starch (SS)	538:556	PB verified soluble starch (SS), colloidal chitosan (CC) peptone, and NaCl as most significant variables affecting chitosanase production.
30022499	4	46	theme	significant	609:619	arg1	variables					621:629	most significant variables	604:629	most significant variables affecting chitosanase production	604:662	PB verified soluble starch (SS), colloidal chitosan (CC) peptone, and NaCl as most significant variables affecting chitosanase production.
30022499	4	46	theme	significant	609:619	arg1	peptone					583:589	colloidal chitosan (CC) peptone	559:589	colloidal chitosan (CC) peptone	559:589	PB verified soluble starch (SS), colloidal chitosan (CC) peptone, and NaCl as most significant variables affecting chitosanase production.
30022499	5	47	theme	NaCl	738:741	arg1	concentrations					699:712	the optimum concentrations	687:712	the optimum concentrations of SS, CC, peptone, and NaCl	687:741	CCD results predicted the optimum concentrations of SS, CC, peptone, and NaCl as 7.8, 7.0, 6.5, and 2.7 g L-1 , respectively to achieve maximum chitosanase activity (21.1 U ml-1 ).
29557775	2	0	theme	western	78:84	arg1	wheatgrass					86:95	western wheatgrass	78:95	western wheatgrass	78:95	rev., isolated from western wheatgrass (Pascopyrum smithii).
29557775	8	1	theme	Rathayibacter	1291:1303	arg1	species					1280:1286	described species	1270:1286	the six other described species of Rathayibacter	1256:1303	Phylogenetic analysis of 16S rRNA gene sequences placed the strains in the genus Rathayibacter and distinguished them from the six other described species of Rathayibacter.
29557775	1	2	dep	nom	53:55	arg1	nov.					47:50	nov.	47:50	nov.	47:50	nov., nom.
29557775	4	3	theme	Washington	500:509	arg1	State					511:515	the Washington State	496:515	the Washington State Mycological Herbarium	496:537	In the 1980s, bacteria resembling Corynebacterium agropyri were isolated from plant samples stored at the Washington State Mycological Herbarium and from a diseased wheatgrass plant collected in Cardwell, Montana, USA.
29557775	10	4	theme	phenotypic	1397:1406	arg1	characterization					1441:1456	phenotypic, chemotaxonomic and phylogenetic characterization	1397:1456	phenotypic, chemotaxonomic and phylogenetic characterization	1397:1456	Based on phenotypic, chemotaxonomic and phylogenetic characterization, it appears that strains CA-1 to CA-12 represent a novel species, and the name Rathayibacter agropyri (non O'Gara 1916) comb.
29557775	3	5	from	consequently	330:341	arg1	Lists					292:296	the Approved Lists	279:296	the Approved Lists of Bacterial Names in 1980 and, consequently, is not recognized as a validly published species	279:391	Aplanobacter agropyri was first described in 1915 by O'Gara and later transferred to the genus Corynebacterium by Burkholder in 1948 but it was not included in the Approved Lists of Bacterial Names in 1980 and, consequently, is not recognized as a validly published species.
29557775	7	6	dep	 0	1045:1046	arg1	 0					1076:1077	 0	1076:1077	 0	1076:1077	The peptidoglycan is type B2γ containing diaminobutyric acid, alanine, glycine and glutamic acid, the cell-wall sugars are rhamnose and mannose, the major respiratory quinone is MK-10, and the major fatty acids are anteiso-15 : 0, anteiso 17 : 0 and iso-16 : 0, all of which are typical of the genus Rathayibacter.
29557775	7	6	dep	 0	1045:1046	arg1	iso-16 					1068:1074	iso-16 	1068:1074	iso-16 	1068:1074	The peptidoglycan is type B2γ containing diaminobutyric acid, alanine, glycine and glutamic acid, the cell-wall sugars are rhamnose and mannose, the major respiratory quinone is MK-10, and the major fatty acids are anteiso-15 : 0, anteiso 17 : 0 and iso-16 : 0, all of which are typical of the genus Rathayibacter.
29557775	7	6	dep	 0	1045:1046	arg1	 0					1061:1062	 0	1061:1062	 0	1061:1062	The peptidoglycan is type B2γ containing diaminobutyric acid, alanine, glycine and glutamic acid, the cell-wall sugars are rhamnose and mannose, the major respiratory quinone is MK-10, and the major fatty acids are anteiso-15 : 0, anteiso 17 : 0 and iso-16 : 0, all of which are typical of the genus Rathayibacter.
29557775	10	7	theme	Rathayibacter	1537:1549	arg1	agropyri					1551:1558	the name Rathayibacter agropyri	1528:1558	the name Rathayibacter agropyri (non O'Gara 1916) comb	1528:1581	Based on phenotypic, chemotaxonomic and phylogenetic characterization, it appears that strains CA-1 to CA-12 represent a novel species, and the name Rathayibacter agropyri (non O'Gara 1916) comb.
29557775	5	8	theme	study	638:642	arg1	framework					620:628	the framework	616:628	the framework of this study	616:642	In the framework of this study, eight additional isolates were recovered from the same herbarium plant samples in 2011.
29557775	7	9	theme	major	1011:1015	arg1	anteiso-15 					1033:1043	anteiso-15 	1033:1043	anteiso-15 	1033:1043	The peptidoglycan is type B2γ containing diaminobutyric acid, alanine, glycine and glutamic acid, the cell-wall sugars are rhamnose and mannose, the major respiratory quinone is MK-10, and the major fatty acids are anteiso-15 : 0, anteiso 17 : 0 and iso-16 : 0, all of which are typical of the genus Rathayibacter.
29557775	7	9	theme	major	1011:1015	arg1	acids					1023:1027	the major fatty acids	1007:1027	the major fatty acids	1007:1027	The peptidoglycan is type B2γ containing diaminobutyric acid, alanine, glycine and glutamic acid, the cell-wall sugars are rhamnose and mannose, the major respiratory quinone is MK-10, and the major fatty acids are anteiso-15 : 0, anteiso 17 : 0 and iso-16 : 0, all of which are typical of the genus Rathayibacter.
29557775	10	10	dep	agropyri	1551:1558	arg1	Gara					1567:1570	Gara	1567:1570	Gara	1567:1570	Based on phenotypic, chemotaxonomic and phylogenetic characterization, it appears that strains CA-1 to CA-12 represent a novel species, and the name Rathayibacter agropyri (non O'Gara 1916) comb.
29557775	4	11	theme	Mycological	517:527	arg1	Herbarium					529:537	the Washington State Mycological Herbarium	496:537	the Washington State Mycological Herbarium	496:537	In the 1980s, bacteria resembling Corynebacterium agropyri were isolated from plant samples stored at the Washington State Mycological Herbarium and from a diseased wheatgrass plant collected in Cardwell, Montana, USA.
29557775	9	12	theme	novel	1373:1377	arg1	species					1379:1385	a novel species	1371:1385	a novel species	1371:1385	DNA-DNA hybridization confirmed that the strains were members of a novel species.
29557775	7	13	theme	fatty	1017:1021	arg1	anteiso-15 					1033:1043	anteiso-15 	1033:1043	anteiso-15 	1033:1043	The peptidoglycan is type B2γ containing diaminobutyric acid, alanine, glycine and glutamic acid, the cell-wall sugars are rhamnose and mannose, the major respiratory quinone is MK-10, and the major fatty acids are anteiso-15 : 0, anteiso 17 : 0 and iso-16 : 0, all of which are typical of the genus Rathayibacter.
29557775	7	13	theme	fatty	1017:1021	arg1	acids					1023:1027	the major fatty acids	1007:1027	the major fatty acids	1007:1027	The peptidoglycan is type B2γ containing diaminobutyric acid, alanine, glycine and glutamic acid, the cell-wall sugars are rhamnose and mannose, the major respiratory quinone is MK-10, and the major fatty acids are anteiso-15 : 0, anteiso 17 : 0 and iso-16 : 0, all of which are typical of the genus Rathayibacter.
29557775	2	14	attach	isolated	64:71	arg2	Pascopyrum					98:107	Pascopyrum	98:107	Pascopyrum	98:107	rev., isolated from western wheatgrass (Pascopyrum smithii).
29557775	2	14	attach	isolated	64:71	arg2	rev.					58:61	rev.	58:61	rev.	58:61	rev., isolated from western wheatgrass (Pascopyrum smithii).
29557775	2	14	attach	isolated	64:71	arg1	wheatgrass					86:95	western wheatgrass	78:95	western wheatgrass	78:95	rev., isolated from western wheatgrass (Pascopyrum smithii).
29557775	4	15	theme	State	511:515	arg1	Herbarium					529:537	the Washington State Mycological Herbarium	496:537	the Washington State Mycological Herbarium	496:537	In the 1980s, bacteria resembling Corynebacterium agropyri were isolated from plant samples stored at the Washington State Mycological Herbarium and from a diseased wheatgrass plant collected in Cardwell, Montana, USA.
29557775	3	16	from	Names	311:315	arg1	1980					320:323	1980	320:323	1980	320:323	Aplanobacter agropyri was first described in 1915 by O'Gara and later transferred to the genus Corynebacterium by Burkholder in 1948 but it was not included in the Approved Lists of Bacterial Names in 1980 and, consequently, is not recognized as a validly published species.
29557775	3	16	from	Names	311:315	arg1	consequently					330:341	consequently	330:341	consequently	330:341	Aplanobacter agropyri was first described in 1915 by O'Gara and later transferred to the genus Corynebacterium by Burkholder in 1948 but it was not included in the Approved Lists of Bacterial Names in 1980 and, consequently, is not recognized as a validly published species.
29557775	3	17	theme	Aplanobacter	119:130	arg1	agropyri					132:139	Aplanobacter agropyri	119:139	Aplanobacter agropyri	119:139	Aplanobacter agropyri was first described in 1915 by O'Gara and later transferred to the genus Corynebacterium by Burkholder in 1948 but it was not included in the Approved Lists of Bacterial Names in 1980 and, consequently, is not recognized as a validly published species.
29557775	7	18	dep	B2γ	844:846	arg1	 0					1045:1046	 0	1045:1046	 0	1045:1046	The peptidoglycan is type B2γ containing diaminobutyric acid, alanine, glycine and glutamic acid, the cell-wall sugars are rhamnose and mannose, the major respiratory quinone is MK-10, and the major fatty acids are anteiso-15 : 0, anteiso 17 : 0 and iso-16 : 0, all of which are typical of the genus Rathayibacter.
29557775	7	18	dep	B2γ	844:846	arg1	17 					1057:1059	anteiso 17 	1049:1059	anteiso 17 	1049:1059	The peptidoglycan is type B2γ containing diaminobutyric acid, alanine, glycine and glutamic acid, the cell-wall sugars are rhamnose and mannose, the major respiratory quinone is MK-10, and the major fatty acids are anteiso-15 : 0, anteiso 17 : 0 and iso-16 : 0, all of which are typical of the genus Rathayibacter.
29557775	3	19	theme	Approved	283:290	arg1	Lists					292:296	the Approved Lists	279:296	the Approved Lists of Bacterial Names in 1980 and, consequently, is not recognized as a validly published species	279:391	Aplanobacter agropyri was first described in 1915 by O'Gara and later transferred to the genus Corynebacterium by Burkholder in 1948 but it was not included in the Approved Lists of Bacterial Names in 1980 and, consequently, is not recognized as a validly published species.
29557775	5	20	from	samples	716:722	arg1	2011					727:730	2011	727:730	2011	727:730	In the framework of this study, eight additional isolates were recovered from the same herbarium plant samples in 2011.
29557775	9	21	theme	species	1379:1385	arg1	members					1360:1366	members	1360:1366	members of a novel species	1360:1385	DNA-DNA hybridization confirmed that the strains were members of a novel species.
29557775	9	21	theme	species	1379:1385	arg1	strains					1347:1353	the strains	1343:1353	the strains	1343:1353	DNA-DNA hybridization confirmed that the strains were members of a novel species.
29557775	7	22	contain	containing	848:857	arg1	B2γ					844:846	type B2γ	839:846	type B2γ containing diaminobutyric acid, alanine, glycine and glutamic acid	839:913	The peptidoglycan is type B2γ containing diaminobutyric acid, alanine, glycine and glutamic acid, the cell-wall sugars are rhamnose and mannose, the major respiratory quinone is MK-10, and the major fatty acids are anteiso-15 : 0, anteiso 17 : 0 and iso-16 : 0, all of which are typical of the genus Rathayibacter.
29557775	7	22	contain	containing	848:857	arg2	acid					910:913	glutamic acid	901:913	glutamic acid	901:913	The peptidoglycan is type B2γ containing diaminobutyric acid, alanine, glycine and glutamic acid, the cell-wall sugars are rhamnose and mannose, the major respiratory quinone is MK-10, and the major fatty acids are anteiso-15 : 0, anteiso 17 : 0 and iso-16 : 0, all of which are typical of the genus Rathayibacter.
29557775	7	22	contain	containing	848:857	arg2	acid					874:877	diaminobutyric acid	859:877	diaminobutyric acid	859:877	The peptidoglycan is type B2γ containing diaminobutyric acid, alanine, glycine and glutamic acid, the cell-wall sugars are rhamnose and mannose, the major respiratory quinone is MK-10, and the major fatty acids are anteiso-15 : 0, anteiso 17 : 0 and iso-16 : 0, all of which are typical of the genus Rathayibacter.
29557775	7	22	contain	containing	848:857	arg2	alanine					880:886	alanine	880:886	alanine	880:886	The peptidoglycan is type B2γ containing diaminobutyric acid, alanine, glycine and glutamic acid, the cell-wall sugars are rhamnose and mannose, the major respiratory quinone is MK-10, and the major fatty acids are anteiso-15 : 0, anteiso 17 : 0 and iso-16 : 0, all of which are typical of the genus Rathayibacter.
29557775	7	22	contain	containing	848:857	arg2	glycine					889:895	glycine	889:895	glycine	889:895	The peptidoglycan is type B2γ containing diaminobutyric acid, alanine, glycine and glutamic acid, the cell-wall sugars are rhamnose and mannose, the major respiratory quinone is MK-10, and the major fatty acids are anteiso-15 : 0, anteiso 17 : 0 and iso-16 : 0, all of which are typical of the genus Rathayibacter.
29557775	7	22	contain	containing	848:857	arg1	peptidoglycan					822:834	The peptidoglycan	818:834	The peptidoglycan	818:834	The peptidoglycan is type B2γ containing diaminobutyric acid, alanine, glycine and glutamic acid, the cell-wall sugars are rhamnose and mannose, the major respiratory quinone is MK-10, and the major fatty acids are anteiso-15 : 0, anteiso 17 : 0 and iso-16 : 0, all of which are typical of the genus Rathayibacter.
29557775	11	23	dep	nom	1590:1592	arg1	nov.					1584:1587	nov.	1584:1587	nov.	1584:1587	nov., nom.
29557775	9	24	theme	DNA-DNA	1306:1312	arg1	hybridization					1314:1326	DNA-DNA hybridization	1306:1326	DNA-DNA hybridization	1306:1326	DNA-DNA hybridization confirmed that the strains were members of a novel species.
29557775	10	25	theme	CA-1	1483:1486	arg1	strains					1475:1481	strains	1475:1481	strains CA-1 to CA-12	1475:1495	Based on phenotypic, chemotaxonomic and phylogenetic characterization, it appears that strains CA-1 to CA-12 represent a novel species, and the name Rathayibacter agropyri (non O'Gara 1916) comb.
29557775	3	26	from	1980	320:323	arg1	Lists					292:296	the Approved Lists	279:296	the Approved Lists of Bacterial Names in 1980 and, consequently, is not recognized as a validly published species	279:391	Aplanobacter agropyri was first described in 1915 by O'Gara and later transferred to the genus Corynebacterium by Burkholder in 1948 but it was not included in the Approved Lists of Bacterial Names in 1980 and, consequently, is not recognized as a validly published species.
29557775	3	27	dep	consequently	330:341	arg1	recognized					351:360	recognized	351:360	is not recognized as a validly published species	344:391	Aplanobacter agropyri was first described in 1915 by O'Gara and later transferred to the genus Corynebacterium by Burkholder in 1948 but it was not included in the Approved Lists of Bacterial Names in 1980 and, consequently, is not recognized as a validly published species.
29557775	4	28	theme	Corynebacterium	428:442	arg1	agropyri					444:451	Corynebacterium agropyri	428:451	Corynebacterium agropyri	428:451	In the 1980s, bacteria resembling Corynebacterium agropyri were isolated from plant samples stored at the Washington State Mycological Herbarium and from a diseased wheatgrass plant collected in Cardwell, Montana, USA.
29557775	10	29	theme	novel	1509:1513	arg1	species					1515:1521	a novel species	1507:1521	a novel species	1507:1521	Based on phenotypic, chemotaxonomic and phylogenetic characterization, it appears that strains CA-1 to CA-12 represent a novel species, and the name Rathayibacter agropyri (non O'Gara 1916) comb.
29557775	3	30	theme	Bacterial	301:309	arg1	Names					311:315	Bacterial Names	301:315	Bacterial Names in 1980 and, consequently, is not recognized as a validly published species	301:391	Aplanobacter agropyri was first described in 1915 by O'Gara and later transferred to the genus Corynebacterium by Burkholder in 1948 but it was not included in the Approved Lists of Bacterial Names in 1980 and, consequently, is not recognized as a validly published species.
29557775	4	31	theme	plant	472:476	arg1	samples					478:484	plant samples	472:484	plant samples stored at the Washington State Mycological Herbarium	472:537	In the 1980s, bacteria resembling Corynebacterium agropyri were isolated from plant samples stored at the Washington State Mycological Herbarium and from a diseased wheatgrass plant collected in Cardwell, Montana, USA.
29557775	8	32	theme	16S	1158:1160	arg1	sequences					1172:1180	16S rRNA gene sequences	1158:1180	16S rRNA gene sequences	1158:1180	Phylogenetic analysis of 16S rRNA gene sequences placed the strains in the genus Rathayibacter and distinguished them from the six other described species of Rathayibacter.
29557775	7	33	theme	diaminobutyric	859:872	arg1	acid					874:877	diaminobutyric acid	859:877	diaminobutyric acid	859:877	The peptidoglycan is type B2γ containing diaminobutyric acid, alanine, glycine and glutamic acid, the cell-wall sugars are rhamnose and mannose, the major respiratory quinone is MK-10, and the major fatty acids are anteiso-15 : 0, anteiso 17 : 0 and iso-16 : 0, all of which are typical of the genus Rathayibacter.
29557775	4	34	attach	isolated	458:465	arg1	plant					570:574	a diseased wheatgrass plant	548:574	a diseased wheatgrass plant collected in Cardwell, Montana, USA	548:610	In the 1980s, bacteria resembling Corynebacterium agropyri were isolated from plant samples stored at the Washington State Mycological Herbarium and from a diseased wheatgrass plant collected in Cardwell, Montana, USA.
29557775	4	34	attach	isolated	458:465	arg1	samples					478:484	plant samples	472:484	plant samples stored at the Washington State Mycological Herbarium	472:537	In the 1980s, bacteria resembling Corynebacterium agropyri were isolated from plant samples stored at the Washington State Mycological Herbarium and from a diseased wheatgrass plant collected in Cardwell, Montana, USA.
29557775	4	34	attach	isolated	458:465	arg2	bacteria					408:415	bacteria	408:415	bacteria resembling Corynebacterium agropyri	408:451	In the 1980s, bacteria resembling Corynebacterium agropyri were isolated from plant samples stored at the Washington State Mycological Herbarium and from a diseased wheatgrass plant collected in Cardwell, Montana, USA.
29557775	12	35	theme	104101T	1644:1650	arg1	TSD-78T					1658:1664	=DSM 104101T;=ATCC TSD-78T	1639:1664	=DSM 104101T;=ATCC TSD-78T	1639:1664	rev. is proposed; the type strain is CA-4T (=DSM 104101T;=ATCC TSD-78T).
29557775	12	35	theme	104101T	1644:1650	arg1	CA-4T					1632:1636	CA-4T	1632:1636	CA-4T (=DSM 104101T;=ATCC TSD-78T)	1632:1665	rev. is proposed; the type strain is CA-4T (=DSM 104101T;=ATCC TSD-78T).
29557775	4	36	theme	wheatgrass	559:568	arg1	plant					570:574	a diseased wheatgrass plant	548:574	a diseased wheatgrass plant collected in Cardwell, Montana, USA	548:610	In the 1980s, bacteria resembling Corynebacterium agropyri were isolated from plant samples stored at the Washington State Mycological Herbarium and from a diseased wheatgrass plant collected in Cardwell, Montana, USA.
29557775	3	37	theme	Names	311:315	arg1	Lists					292:296	the Approved Lists	279:296	the Approved Lists of Bacterial Names in 1980 and, consequently, is not recognized as a validly published species	279:391	Aplanobacter agropyri was first described in 1915 by O'Gara and later transferred to the genus Corynebacterium by Burkholder in 1948 but it was not included in the Approved Lists of Bacterial Names in 1980 and, consequently, is not recognized as a validly published species.
29557775	7	38	theme	major	967:971	arg1	MK-10					996:1000	MK-10	996:1000	MK-10	996:1000	The peptidoglycan is type B2γ containing diaminobutyric acid, alanine, glycine and glutamic acid, the cell-wall sugars are rhamnose and mannose, the major respiratory quinone is MK-10, and the major fatty acids are anteiso-15 : 0, anteiso 17 : 0 and iso-16 : 0, all of which are typical of the genus Rathayibacter.
29557775	7	38	theme	major	967:971	arg1	quinone					985:991	the major respiratory quinone	963:991	the major respiratory quinone	963:991	The peptidoglycan is type B2γ containing diaminobutyric acid, alanine, glycine and glutamic acid, the cell-wall sugars are rhamnose and mannose, the major respiratory quinone is MK-10, and the major fatty acids are anteiso-15 : 0, anteiso 17 : 0 and iso-16 : 0, all of which are typical of the genus Rathayibacter.
29557775	8	39	theme	genus	1208:1212	arg1	Rathayibacter					1214:1226	the genus Rathayibacter	1204:1226	the genus Rathayibacter	1204:1226	Phylogenetic analysis of 16S rRNA gene sequences placed the strains in the genus Rathayibacter and distinguished them from the six other described species of Rathayibacter.
29557775	0	40	dep	comb	41:44	arg1	agropyri					14:21	agropyri	14:21	agropyri	14:21	Rathayibacter agropyri (non O'Gara 1916) comb.
29557775	4	41	theme	diseased	550:557	arg1	plant					570:574	a diseased wheatgrass plant	548:574	a diseased wheatgrass plant collected in Cardwell, Montana, USA	548:610	In the 1980s, bacteria resembling Corynebacterium agropyri were isolated from plant samples stored at the Washington State Mycological Herbarium and from a diseased wheatgrass plant collected in Cardwell, Montana, USA.
29557775	3	42	theme	genus	208:212	arg1	Corynebacterium					214:228	the genus Corynebacterium	204:228	the genus Corynebacterium	204:228	Aplanobacter agropyri was first described in 1915 by O'Gara and later transferred to the genus Corynebacterium by Burkholder in 1948 but it was not included in the Approved Lists of Bacterial Names in 1980 and, consequently, is not recognized as a validly published species.
29557775	10	43	theme	name	1532:1535	arg1	agropyri					1551:1558	the name Rathayibacter agropyri	1528:1558	the name Rathayibacter agropyri (non O'Gara 1916) comb	1528:1581	Based on phenotypic, chemotaxonomic and phylogenetic characterization, it appears that strains CA-1 to CA-12 represent a novel species, and the name Rathayibacter agropyri (non O'Gara 1916) comb.
29557775	10	44	theme	agropyri	1551:1558	arg1	comb					1578:1581	the name Rathayibacter agropyri (non O'Gara 1916) comb	1528:1581	the name Rathayibacter agropyri (non O'Gara 1916) comb	1528:1581	Based on phenotypic, chemotaxonomic and phylogenetic characterization, it appears that strains CA-1 to CA-12 represent a novel species, and the name Rathayibacter agropyri (non O'Gara 1916) comb.
29557775	8	45	theme	Phylogenetic	1133:1144	arg1	analysis					1146:1153	Phylogenetic analysis	1133:1153	Phylogenetic analysis of 16S rRNA gene sequences	1133:1180	Phylogenetic analysis of 16S rRNA gene sequences placed the strains in the genus Rathayibacter and distinguished them from the six other described species of Rathayibacter.
29557775	5	46	theme	same	695:698	arg1	samples					716:722	the same herbarium plant samples	691:722	the same herbarium plant samples in 2011	691:730	In the framework of this study, eight additional isolates were recovered from the same herbarium plant samples in 2011.
29557775	7	47	theme	respiratory	973:983	arg1	MK-10					996:1000	MK-10	996:1000	MK-10	996:1000	The peptidoglycan is type B2γ containing diaminobutyric acid, alanine, glycine and glutamic acid, the cell-wall sugars are rhamnose and mannose, the major respiratory quinone is MK-10, and the major fatty acids are anteiso-15 : 0, anteiso 17 : 0 and iso-16 : 0, all of which are typical of the genus Rathayibacter.
29557775	7	47	theme	respiratory	973:983	arg1	quinone					985:991	the major respiratory quinone	963:991	the major respiratory quinone	963:991	The peptidoglycan is type B2γ containing diaminobutyric acid, alanine, glycine and glutamic acid, the cell-wall sugars are rhamnose and mannose, the major respiratory quinone is MK-10, and the major fatty acids are anteiso-15 : 0, anteiso 17 : 0 and iso-16 : 0, all of which are typical of the genus Rathayibacter.
29557775	3	48	from	Lists	292:296	arg1	1980					320:323	1980	320:323	1980	320:323	Aplanobacter agropyri was first described in 1915 by O'Gara and later transferred to the genus Corynebacterium by Burkholder in 1948 but it was not included in the Approved Lists of Bacterial Names in 1980 and, consequently, is not recognized as a validly published species.
29557775	3	48	from	Lists	292:296	arg1	consequently					330:341	consequently	330:341	consequently	330:341	Aplanobacter agropyri was first described in 1915 by O'Gara and later transferred to the genus Corynebacterium by Burkholder in 1948 but it was not included in the Approved Lists of Bacterial Names in 1980 and, consequently, is not recognized as a validly published species.
29557775	7	49	theme	anteiso	1049:1055	arg1	 0					1045:1046	 0	1045:1046	 0	1045:1046	The peptidoglycan is type B2γ containing diaminobutyric acid, alanine, glycine and glutamic acid, the cell-wall sugars are rhamnose and mannose, the major respiratory quinone is MK-10, and the major fatty acids are anteiso-15 : 0, anteiso 17 : 0 and iso-16 : 0, all of which are typical of the genus Rathayibacter.
29557775	7	49	theme	anteiso	1049:1055	arg1	17 					1057:1059	anteiso 17 	1049:1059	anteiso 17 	1049:1059	The peptidoglycan is type B2γ containing diaminobutyric acid, alanine, glycine and glutamic acid, the cell-wall sugars are rhamnose and mannose, the major respiratory quinone is MK-10, and the major fatty acids are anteiso-15 : 0, anteiso 17 : 0 and iso-16 : 0, all of which are typical of the genus Rathayibacter.
29557775	5	50	theme	additional	651:660	arg1	isolates					662:669	eight additional isolates	645:669	eight additional isolates	645:669	In the framework of this study, eight additional isolates were recovered from the same herbarium plant samples in 2011.
29557775	5	51	theme	herbarium	700:708	arg1	samples					716:722	the same herbarium plant samples	691:722	the same herbarium plant samples in 2011	691:730	In the framework of this study, eight additional isolates were recovered from the same herbarium plant samples in 2011.
29557775	12	52	theme	=ATCC	1652:1656	arg1	TSD-78T					1658:1664	=DSM 104101T;=ATCC TSD-78T	1639:1664	=DSM 104101T;=ATCC TSD-78T	1639:1664	rev. is proposed; the type strain is CA-4T (=DSM 104101T;=ATCC TSD-78T).
29557775	12	52	theme	=ATCC	1652:1656	arg1	CA-4T					1632:1636	CA-4T	1632:1636	CA-4T (=DSM 104101T;=ATCC TSD-78T)	1632:1665	rev. is proposed; the type strain is CA-4T (=DSM 104101T;=ATCC TSD-78T).
29557775	8	53	theme	other	1264:1268	arg1	species					1280:1286	described species	1270:1286	the six other described species of Rathayibacter	1256:1303	Phylogenetic analysis of 16S rRNA gene sequences placed the strains in the genus Rathayibacter and distinguished them from the six other described species of Rathayibacter.
29557775	5	54	theme	plant	710:714	arg1	samples					716:722	the same herbarium plant samples	691:722	the same herbarium plant samples in 2011	691:730	In the framework of this study, eight additional isolates were recovered from the same herbarium plant samples in 2011.
29557775	7	55	theme	cell-wall	920:928	arg1	sugars					930:935	the cell-wall sugars	916:935	the cell-wall sugars	916:935	The peptidoglycan is type B2γ containing diaminobutyric acid, alanine, glycine and glutamic acid, the cell-wall sugars are rhamnose and mannose, the major respiratory quinone is MK-10, and the major fatty acids are anteiso-15 : 0, anteiso 17 : 0 and iso-16 : 0, all of which are typical of the genus Rathayibacter.
29557775	7	55	theme	cell-wall	920:928	arg1	rhamnose					941:948	rhamnose	941:948	rhamnose	941:948	The peptidoglycan is type B2γ containing diaminobutyric acid, alanine, glycine and glutamic acid, the cell-wall sugars are rhamnose and mannose, the major respiratory quinone is MK-10, and the major fatty acids are anteiso-15 : 0, anteiso 17 : 0 and iso-16 : 0, all of which are typical of the genus Rathayibacter.
29557775	10	56	theme	phylogenetic	1428:1439	arg1	characterization					1441:1456	phenotypic, chemotaxonomic and phylogenetic characterization	1397:1456	phenotypic, chemotaxonomic and phylogenetic characterization	1397:1456	Based on phenotypic, chemotaxonomic and phylogenetic characterization, it appears that strains CA-1 to CA-12 represent a novel species, and the name Rathayibacter agropyri (non O'Gara 1916) comb.
29557775	7	57	theme	type	839:842	arg1	peptidoglycan					822:834	The peptidoglycan	818:834	The peptidoglycan	818:834	The peptidoglycan is type B2γ containing diaminobutyric acid, alanine, glycine and glutamic acid, the cell-wall sugars are rhamnose and mannose, the major respiratory quinone is MK-10, and the major fatty acids are anteiso-15 : 0, anteiso 17 : 0 and iso-16 : 0, all of which are typical of the genus Rathayibacter.
29557775	7	57	theme	type	839:842	arg1	B2γ					844:846	type B2γ	839:846	type B2γ containing diaminobutyric acid, alanine, glycine and glutamic acid	839:913	The peptidoglycan is type B2γ containing diaminobutyric acid, alanine, glycine and glutamic acid, the cell-wall sugars are rhamnose and mannose, the major respiratory quinone is MK-10, and the major fatty acids are anteiso-15 : 0, anteiso 17 : 0 and iso-16 : 0, all of which are typical of the genus Rathayibacter.
29557775	12	58	theme	=DSM	1639:1642	arg1	TSD-78T					1658:1664	=DSM 104101T;=ATCC TSD-78T	1639:1664	=DSM 104101T;=ATCC TSD-78T	1639:1664	rev. is proposed; the type strain is CA-4T (=DSM 104101T;=ATCC TSD-78T).
29557775	12	58	theme	=DSM	1639:1642	arg1	CA-4T					1632:1636	CA-4T	1632:1636	CA-4T (=DSM 104101T;=ATCC TSD-78T)	1632:1665	rev. is proposed; the type strain is CA-4T (=DSM 104101T;=ATCC TSD-78T).
29557775	7	59	theme	genus	1112:1116	arg1	Rathayibacter					1118:1130	the genus Rathayibacter	1108:1130	the genus Rathayibacter	1108:1130	The peptidoglycan is type B2γ containing diaminobutyric acid, alanine, glycine and glutamic acid, the cell-wall sugars are rhamnose and mannose, the major respiratory quinone is MK-10, and the major fatty acids are anteiso-15 : 0, anteiso 17 : 0 and iso-16 : 0, all of which are typical of the genus Rathayibacter.
29557775	8	60	theme	described	1270:1278	arg1	species					1280:1286	described species	1270:1286	the six other described species of Rathayibacter	1256:1303	Phylogenetic analysis of 16S rRNA gene sequences placed the strains in the genus Rathayibacter and distinguished them from the six other described species of Rathayibacter.
29557775	12	61	theme	type	1617:1620	arg1	strain					1622:1627	the type strain	1613:1627	the type strain	1613:1627	rev. is proposed; the type strain is CA-4T (=DSM 104101T;=ATCC TSD-78T).
29557775	12	61	theme	type	1617:1620	arg1	CA-4T					1632:1636	CA-4T	1632:1636	CA-4T (=DSM 104101T;=ATCC TSD-78T)	1632:1665	rev. is proposed; the type strain is CA-4T (=DSM 104101T;=ATCC TSD-78T).
29557775	7	62	theme	Rathayibacter	1118:1130	arg1	typical					1097:1103	typical	1097:1103	typical	1097:1103	The peptidoglycan is type B2γ containing diaminobutyric acid, alanine, glycine and glutamic acid, the cell-wall sugars are rhamnose and mannose, the major respiratory quinone is MK-10, and the major fatty acids are anteiso-15 : 0, anteiso 17 : 0 and iso-16 : 0, all of which are typical of the genus Rathayibacter.
29557775	8	63	theme	rRNA	1162:1165	arg1	sequences					1172:1180	16S rRNA gene sequences	1158:1180	16S rRNA gene sequences	1158:1180	Phylogenetic analysis of 16S rRNA gene sequences placed the strains in the genus Rathayibacter and distinguished them from the six other described species of Rathayibacter.
29557775	0	64	dep	agropyri	14:21	arg1	Gara					30:33	Gara	30:33	Gara	30:33	Rathayibacter agropyri (non O'Gara 1916) comb.
29557775	3	65	theme	published	375:383	arg1	species					385:391	a validly published species	365:391	a validly published species	365:391	Aplanobacter agropyri was first described in 1915 by O'Gara and later transferred to the genus Corynebacterium by Burkholder in 1948 but it was not included in the Approved Lists of Bacterial Names in 1980 and, consequently, is not recognized as a validly published species.
29557775	8	66	theme	sequences	1172:1180	arg1	analysis					1146:1153	Phylogenetic analysis	1133:1153	Phylogenetic analysis of 16S rRNA gene sequences	1133:1180	Phylogenetic analysis of 16S rRNA gene sequences placed the strains in the genus Rathayibacter and distinguished them from the six other described species of Rathayibacter.
29557775	10	67	theme	chemotaxonomic	1409:1422	arg1	characterization					1441:1456	phenotypic, chemotaxonomic and phylogenetic characterization	1397:1456	phenotypic, chemotaxonomic and phylogenetic characterization	1397:1456	Based on phenotypic, chemotaxonomic and phylogenetic characterization, it appears that strains CA-1 to CA-12 represent a novel species, and the name Rathayibacter agropyri (non O'Gara 1916) comb.
29557775	7	68	theme	glutamic	901:908	arg1	acid					910:913	glutamic acid	901:913	glutamic acid	901:913	The peptidoglycan is type B2γ containing diaminobutyric acid, alanine, glycine and glutamic acid, the cell-wall sugars are rhamnose and mannose, the major respiratory quinone is MK-10, and the major fatty acids are anteiso-15 : 0, anteiso 17 : 0 and iso-16 : 0, all of which are typical of the genus Rathayibacter.
29557775	8	69	theme	gene	1167:1170	arg1	sequences					1172:1180	16S rRNA gene sequences	1158:1180	16S rRNA gene sequences	1158:1180	Phylogenetic analysis of 16S rRNA gene sequences placed the strains in the genus Rathayibacter and distinguished them from the six other described species of Rathayibacter.
30154337	0	0	theme	Sugars	73:78	arg1	Intake					41:46	Intake	41:46	Intake of Added Sugars and Free Sugars	41:78	A Disaggregation Methodology to Estimate Intake of Added Sugars and Free Sugars: An Illustration from the UK National Diet and Nutrition Survey.
30154337	2	1	theme	cellular	471:478	arg1	structure					480:488	the cellular structure	467:488	the cellular structure of foods	467:497	Furthermore, analytical methods to identify those sugars which are not naturally incorporated into the cellular structure of foods are lacking, thus free and added sugars are difficult to estimate in an efficient and accurate way.
30154337	2	2	theme	efficient	571:579	arg1	way					594:596	an efficient and accurate way	568:596	an efficient and accurate way	568:596	Furthermore, analytical methods to identify those sugars which are not naturally incorporated into the cellular structure of foods are lacking, thus free and added sugars are difficult to estimate in an efficient and accurate way.
30154337	0	3	theme	Free	68:71	arg1	Sugars					73:78	Free Sugars	68:78	Free Sugars	68:78	A Disaggregation Methodology to Estimate Intake of Added Sugars and Free Sugars: An Illustration from the UK National Diet and Nutrition Survey.
30154337	1	4	theme	nutritional	320:330	arg1	surveillance					332:343	nutritional surveillance	320:343	nutritional surveillance	320:343	Various and inconsistent definitions for free and added sugars are used in the consideration and assessment of dietary intakes across public health, presenting challenges for nutritional surveillance, research, and policy.
30154337	0	5	from	Survey	137:142	arg1	Illustration					84:95	An Illustration	81:95	An Illustration from the UK National Diet and Nutrition Survey	81:142	A Disaggregation Methodology to Estimate Intake of Added Sugars and Free Sugars: An Illustration from the UK National Diet and Nutrition Survey.
30154337	3	6	theme	accurate	636:643	arg1	method					645:650	a feasible and accurate method	621:650	a feasible and accurate method that can be applied flexibly to different definitions	621:704	We aimed to establish a feasible and accurate method that can be applied flexibly to different definitions.
30154337	6	7	theme	efficient	1075:1083	arg1	assessment					1085:1094	a standardized and efficient assessment	1056:1094	a standardized and efficient assessment of added and free sugars	1056:1119	Our approach facilitates a standardized and efficient assessment of added and free sugars, offering benefit and potential for nutrition research and surveillance, and for the food industry, for example to support sugar reduction and reformulation agendas.
30154337	4	8	theme	recipe	716:721	arg1	disaggregation					723:736	recipe disaggregation	716:736	recipe disaggregation	716:736	Based on recipe disaggregation, our method involved five steps and showed good repeatability and validity.
30154337	6	9	theme	nutrition	1157:1165	arg1	research					1167:1174	nutrition research	1157:1174	nutrition research	1157:1174	Our approach facilitates a standardized and efficient assessment of added and free sugars, offering benefit and potential for nutrition research and surveillance, and for the food industry, for example to support sugar reduction and reformulation agendas.
30154337	3	10	theme	different	684:692	arg1	definitions					694:704	different definitions	684:704	different definitions	684:704	We aimed to establish a feasible and accurate method that can be applied flexibly to different definitions.
30154337	5	11	theme	Sugars	833:838	arg1	Database					840:847	The resulting Free Sugars Database	814:847	The resulting Free Sugars Database	814:847	The resulting Free Sugars Database provided data for seven components of sugars; (1) table sugar; (2) other sugars; (3) honey; (4) fruit juice; (5) fruit puree; (6) dried fruit; and (7) stewed fruit, for ~9000 foods.
30154337	1	12	theme	Various	145:151	arg1	definitions					170:180	Various and inconsistent definitions	145:180	Various and inconsistent definitions for free and added sugars	145:206	Various and inconsistent definitions for free and added sugars are used in the consideration and assessment of dietary intakes across public health, presenting challenges for nutritional surveillance, research, and policy.
30154337	2	13	theme	foods	493:497	arg1	structure					480:488	the cellular structure	467:488	the cellular structure of foods	467:497	Furthermore, analytical methods to identify those sugars which are not naturally incorporated into the cellular structure of foods are lacking, thus free and added sugars are difficult to estimate in an efficient and accurate way.
30154337	5	14	theme	fruit	962:966	arg1	puree					968:972	(5) fruit puree	958:972	(5) fruit puree	958:972	The resulting Free Sugars Database provided data for seven components of sugars; (1) table sugar; (2) other sugars; (3) honey; (4) fruit juice; (5) fruit puree; (6) dried fruit; and (7) stewed fruit, for ~9000 foods.
30154337	6	15	theme	standardized	1058:1069	arg1	assessment					1085:1094	a standardized and efficient assessment	1056:1094	a standardized and efficient assessment of added and free sugars	1056:1119	Our approach facilitates a standardized and efficient assessment of added and free sugars, offering benefit and potential for nutrition research and surveillance, and for the food industry, for example to support sugar reduction and reformulation agendas.
30154337	5	16	theme	Free	828:831	arg1	Sugars					833:838	The resulting Free Sugars	814:838	The resulting Free Sugars Database	814:847	The resulting Free Sugars Database provided data for seven components of sugars; (1) table sugar; (2) other sugars; (3) honey; (4) fruit juice; (5) fruit puree; (6) dried fruit; and (7) stewed fruit, for ~9000 foods.
30154337	0	17	theme	Disaggregation	2:15	arg1	Methodology					17:27	A Disaggregation Methodology	0:27	A Disaggregation Methodology	0:27	A Disaggregation Methodology to Estimate Intake of Added Sugars and Free Sugars: An Illustration from the UK National Diet and Nutrition Survey.
30154337	2	18	theme	accurate	585:592	arg1	way					594:596	an efficient and accurate way	568:596	an efficient and accurate way	568:596	Furthermore, analytical methods to identify those sugars which are not naturally incorporated into the cellular structure of foods are lacking, thus free and added sugars are difficult to estimate in an efficient and accurate way.
30154337	6	19	theme	sugars	1114:1119	arg1	assessment					1085:1094	a standardized and efficient assessment	1056:1094	a standardized and efficient assessment of added and free sugars	1056:1119	Our approach facilitates a standardized and efficient assessment of added and free sugars, offering benefit and potential for nutrition research and surveillance, and for the food industry, for example to support sugar reduction and reformulation agendas.
30154337	1	20	theme	inconsistent	157:168	arg1	definitions					170:180	Various and inconsistent definitions	145:180	Various and inconsistent definitions for free and added sugars	145:206	Various and inconsistent definitions for free and added sugars are used in the consideration and assessment of dietary intakes across public health, presenting challenges for nutritional surveillance, research, and policy.
30154337	3	21	theme	feasible	623:630	arg1	method					645:650	a feasible and accurate method	621:650	a feasible and accurate method that can be applied flexibly to different definitions	621:704	We aimed to establish a feasible and accurate method that can be applied flexibly to different definitions.
30154337	1	22	theme	dietary	256:262	arg1	intakes					264:270	dietary intakes	256:270	dietary intakes	256:270	Various and inconsistent definitions for free and added sugars are used in the consideration and assessment of dietary intakes across public health, presenting challenges for nutritional surveillance, research, and policy.
30154337	0	23	theme	UK	106:107	arg1	Diet					118:121	the UK National Diet and Nutrition Survey	102:142	Diet	118:121	A Disaggregation Methodology to Estimate Intake of Added Sugars and Free Sugars: An Illustration from the UK National Diet and Nutrition Survey.
30154337	4	24	theme	good	781:784	arg1	repeatability					786:798	good repeatability	781:798	good repeatability	781:798	Based on recipe disaggregation, our method involved five steps and showed good repeatability and validity.
30154337	2	25	theme	analytical	381:390	arg1	methods					392:398	analytical methods	381:398	analytical methods to identify those sugars which are not naturally incorporated into the cellular structure of foods	381:497	Furthermore, analytical methods to identify those sugars which are not naturally incorporated into the cellular structure of foods are lacking, thus free and added sugars are difficult to estimate in an efficient and accurate way.
30154337	6	26	dep	sugars	1114:1119	arg1	free					1109:1112	free	1109:1112	free	1109:1112	Our approach facilitates a standardized and efficient assessment of added and free sugars, offering benefit and potential for nutrition research and surveillance, and for the food industry, for example to support sugar reduction and reformulation agendas.
30154337	6	26	dep	sugars	1114:1119	arg1	added					1099:1103	added	1099:1103	added	1099:1103	Our approach facilitates a standardized and efficient assessment of added and free sugars, offering benefit and potential for nutrition research and surveillance, and for the food industry, for example to support sugar reduction and reformulation agendas.
30154337	1	27	theme	intakes	264:270	arg1	consideration					224:236	consideration	224:236	consideration	224:236	Various and inconsistent definitions for free and added sugars are used in the consideration and assessment of dietary intakes across public health, presenting challenges for nutritional surveillance, research, and policy.
30154337	1	27	theme	intakes	264:270	arg1	assessment					242:251	assessment	242:251	assessment	242:251	Various and inconsistent definitions for free and added sugars are used in the consideration and assessment of dietary intakes across public health, presenting challenges for nutritional surveillance, research, and policy.
30154337	5	28	theme	fruit	945:949	arg1	juice					951:955	(4) fruit juice	941:955	(4) fruit juice	941:955	The resulting Free Sugars Database provided data for seven components of sugars; (1) table sugar; (2) other sugars; (3) honey; (4) fruit juice; (5) fruit puree; (6) dried fruit; and (7) stewed fruit, for ~9000 foods.
30154337	5	29	theme	table	899:903	arg1	sugar					905:909	(1) table sugar	895:909	(1) table sugar	895:909	The resulting Free Sugars Database provided data for seven components of sugars; (1) table sugar; (2) other sugars; (3) honey; (4) fruit juice; (5) fruit puree; (6) dried fruit; and (7) stewed fruit, for ~9000 foods.
30154337	1	30	theme	free	186:189	arg1	sugars					201:206	free and added sugars	186:206	free and added sugars	186:206	Various and inconsistent definitions for free and added sugars are used in the consideration and assessment of dietary intakes across public health, presenting challenges for nutritional surveillance, research, and policy.
30154337	5	31	theme	stewed	1000:1005	arg1	fruit					1007:1011	(7) stewed fruit	996:1011	(7) stewed fruit	996:1011	The resulting Free Sugars Database provided data for seven components of sugars; (1) table sugar; (2) other sugars; (3) honey; (4) fruit juice; (5) fruit puree; (6) dried fruit; and (7) stewed fruit, for ~9000 foods.
30154337	2	32	theme	free	517:520	arg1	sugars					532:537	thus free and added sugars	512:537	thus free and added sugars	512:537	Furthermore, analytical methods to identify those sugars which are not naturally incorporated into the cellular structure of foods are lacking, thus free and added sugars are difficult to estimate in an efficient and accurate way.
30154337	2	33	dep	methods	392:398	arg1	identify					403:410	identify	403:410	to identify those sugars which are not naturally incorporated into the cellular structure of foods	400:497	Furthermore, analytical methods to identify those sugars which are not naturally incorporated into the cellular structure of foods are lacking, thus free and added sugars are difficult to estimate in an efficient and accurate way.
30154337	5	34	theme	resulting	818:826	arg1	Sugars					833:838	The resulting Free Sugars	814:838	The resulting Free Sugars Database	814:847	The resulting Free Sugars Database provided data for seven components of sugars; (1) table sugar; (2) other sugars; (3) honey; (4) fruit juice; (5) fruit puree; (6) dried fruit; and (7) stewed fruit, for ~9000 foods.
30154337	1	35	theme	public	279:284	arg1	health					286:291	public health	279:291	public health	279:291	Various and inconsistent definitions for free and added sugars are used in the consideration and assessment of dietary intakes across public health, presenting challenges for nutritional surveillance, research, and policy.
30154337	0	36	theme	National	109:116	arg1	Diet					118:121	the UK National Diet and Nutrition Survey	102:142	Diet	118:121	A Disaggregation Methodology to Estimate Intake of Added Sugars and Free Sugars: An Illustration from the UK National Diet and Nutrition Survey.
30154337	1	37	theme	added	195:199	arg1	sugars					201:206	free and added sugars	186:206	free and added sugars	186:206	Various and inconsistent definitions for free and added sugars are used in the consideration and assessment of dietary intakes across public health, presenting challenges for nutritional surveillance, research, and policy.
30154337	0	38	theme	Added	51:55	arg1	Sugars					57:62	Added Sugars	51:62	Added Sugars	51:62	A Disaggregation Methodology to Estimate Intake of Added Sugars and Free Sugars: An Illustration from the UK National Diet and Nutrition Survey.
30154337	5	39	theme	dried	979:983	arg1	fruit					985:989	(6) dried fruit	975:989	(6) dried fruit	975:989	The resulting Free Sugars Database provided data for seven components of sugars; (1) table sugar; (2) other sugars; (3) honey; (4) fruit juice; (5) fruit puree; (6) dried fruit; and (7) stewed fruit, for ~9000 foods.
30154337	0	40	theme	Nutrition	127:135	arg1	Survey					137:142	the UK National Diet and Nutrition Survey	102:142	Survey	137:142	A Disaggregation Methodology to Estimate Intake of Added Sugars and Free Sugars: An Illustration from the UK National Diet and Nutrition Survey.
30154337	6	41	theme	food	1206:1209	arg1	industry					1211:1218	the food industry	1202:1218	the food industry	1202:1218	Our approach facilitates a standardized and efficient assessment of added and free sugars, offering benefit and potential for nutrition research and surveillance, and for the food industry, for example to support sugar reduction and reformulation agendas.
30154337	2	42	theme	added	526:530	arg1	sugars					532:537	thus free and added sugars	512:537	thus free and added sugars	512:537	Furthermore, analytical methods to identify those sugars which are not naturally incorporated into the cellular structure of foods are lacking, thus free and added sugars are difficult to estimate in an efficient and accurate way.
30154337	0	43	dep	Estimate	32:39	arg1	Illustration					84:95	An Illustration	81:95	An Illustration from the UK National Diet and Nutrition Survey	81:142	A Disaggregation Methodology to Estimate Intake of Added Sugars and Free Sugars: An Illustration from the UK National Diet and Nutrition Survey.
30154337	6	44	theme	sugar	1244:1248	arg1	reduction					1250:1258	sugar reduction and reformulation agendas	1244:1284	reduction	1250:1258	Our approach facilitates a standardized and efficient assessment of added and free sugars, offering benefit and potential for nutrition research and surveillance, and for the food industry, for example to support sugar reduction and reformulation agendas.
30154337	1	45	used	used	212:215	arg2	definitions					170:180	Various and inconsistent definitions	145:180	Various and inconsistent definitions for free and added sugars	145:206	Various and inconsistent definitions for free and added sugars are used in the consideration and assessment of dietary intakes across public health, presenting challenges for nutritional surveillance, research, and policy.
30154337	5	46	theme	sugars	887:892	arg1	sugars					922:927	(2) other sugars	912:927	(2) other sugars	912:927	The resulting Free Sugars Database provided data for seven components of sugars; (1) table sugar; (2) other sugars; (3) honey; (4) fruit juice; (5) fruit puree; (6) dried fruit; and (7) stewed fruit, for ~9000 foods.
30154337	5	46	theme	sugars	887:892	arg1	sugar					905:909	(1) table sugar	895:909	(1) table sugar	895:909	The resulting Free Sugars Database provided data for seven components of sugars; (1) table sugar; (2) other sugars; (3) honey; (4) fruit juice; (5) fruit puree; (6) dried fruit; and (7) stewed fruit, for ~9000 foods.
30154337	5	46	theme	sugars	887:892	arg1	puree					968:972	(5) fruit puree	958:972	(5) fruit puree	958:972	The resulting Free Sugars Database provided data for seven components of sugars; (1) table sugar; (2) other sugars; (3) honey; (4) fruit juice; (5) fruit puree; (6) dried fruit; and (7) stewed fruit, for ~9000 foods.
30154337	5	46	theme	sugars	887:892	arg1	fruit					985:989	(6) dried fruit	975:989	(6) dried fruit	975:989	The resulting Free Sugars Database provided data for seven components of sugars; (1) table sugar; (2) other sugars; (3) honey; (4) fruit juice; (5) fruit puree; (6) dried fruit; and (7) stewed fruit, for ~9000 foods.
30154337	5	46	theme	sugars	887:892	arg1	honey					934:938	(3) honey	930:938	(3) honey	930:938	The resulting Free Sugars Database provided data for seven components of sugars; (1) table sugar; (2) other sugars; (3) honey; (4) fruit juice; (5) fruit puree; (6) dried fruit; and (7) stewed fruit, for ~9000 foods.
30154337	5	46	theme	sugars	887:892	arg1	juice					951:955	(4) fruit juice	941:955	(4) fruit juice	941:955	The resulting Free Sugars Database provided data for seven components of sugars; (1) table sugar; (2) other sugars; (3) honey; (4) fruit juice; (5) fruit puree; (6) dried fruit; and (7) stewed fruit, for ~9000 foods.
30154337	5	46	theme	sugars	887:892	arg1	fruit					1007:1011	(7) stewed fruit	996:1011	(7) stewed fruit	996:1011	The resulting Free Sugars Database provided data for seven components of sugars; (1) table sugar; (2) other sugars; (3) honey; (4) fruit juice; (5) fruit puree; (6) dried fruit; and (7) stewed fruit, for ~9000 foods.
30154337	5	46	theme	sugars	887:892	arg1	components					873:882	seven components	867:882	seven components of sugars	867:892	The resulting Free Sugars Database provided data for seven components of sugars; (1) table sugar; (2) other sugars; (3) honey; (4) fruit juice; (5) fruit puree; (6) dried fruit; and (7) stewed fruit, for ~9000 foods.
30154337	0	47	from	Diet	118:121	arg1	Illustration					84:95	An Illustration	81:95	An Illustration from the UK National Diet and Nutrition Survey	81:142	A Disaggregation Methodology to Estimate Intake of Added Sugars and Free Sugars: An Illustration from the UK National Diet and Nutrition Survey.
30154337	5	48	theme	other	916:920	arg1	sugars					922:927	(2) other sugars	912:927	(2) other sugars	912:927	The resulting Free Sugars Database provided data for seven components of sugars; (1) table sugar; (2) other sugars; (3) honey; (4) fruit juice; (5) fruit puree; (6) dried fruit; and (7) stewed fruit, for ~9000 foods.
30154337	5	49	dep	sugar	905:909	arg1	1					896:896	1	896:896	1	896:896	The resulting Free Sugars Database provided data for seven components of sugars; (1) table sugar; (2) other sugars; (3) honey; (4) fruit juice; (5) fruit puree; (6) dried fruit; and (7) stewed fruit, for ~9000 foods.
30154337	1	50	dep	consideration	224:236	arg1	the					220:222	the	220:222	the	220:222	Various and inconsistent definitions for free and added sugars are used in the consideration and assessment of dietary intakes across public health, presenting challenges for nutritional surveillance, research, and policy.
30154337	5	51	dep	sugars	922:927	arg1	2					913:913	2	913:913	2	913:913	The resulting Free Sugars Database provided data for seven components of sugars; (1) table sugar; (2) other sugars; (3) honey; (4) fruit juice; (5) fruit puree; (6) dried fruit; and (7) stewed fruit, for ~9000 foods.
30154337	5	52	dep	honey	934:938	arg1	3					931:931	3	931:931	3	931:931	The resulting Free Sugars Database provided data for seven components of sugars; (1) table sugar; (2) other sugars; (3) honey; (4) fruit juice; (5) fruit puree; (6) dried fruit; and (7) stewed fruit, for ~9000 foods.
30154337	0	53	theme	Sugars	57:62	arg1	Intake					41:46	Intake	41:46	Intake of Added Sugars and Free Sugars	41:78	A Disaggregation Methodology to Estimate Intake of Added Sugars and Free Sugars: An Illustration from the UK National Diet and Nutrition Survey.
30154337	6	54	theme	reformulation	1264:1276	arg1	agendas					1278:1284	sugar reduction and reformulation agendas	1244:1284	agendas	1278:1284	Our approach facilitates a standardized and efficient assessment of added and free sugars, offering benefit and potential for nutrition research and surveillance, and for the food industry, for example to support sugar reduction and reformulation agendas.
30154337	5	55	dep	juice	951:955	arg1	4					942:942	4	942:942	4	942:942	The resulting Free Sugars Database provided data for seven components of sugars; (1) table sugar; (2) other sugars; (3) honey; (4) fruit juice; (5) fruit puree; (6) dried fruit; and (7) stewed fruit, for ~9000 foods.
31835480	4	0	theme	beads	875:879	arg1	sphericity					857:866	sphericity	857:866	sphericity	857:866	The diameter and sphericity of the beads prepared at pH = 6.0 proved more uniform and had a larger pore size with a good interconnectivity framework.
31835480	4	0	theme	beads	875:879	arg1	diameter					844:851	diameter	844:851	diameter	844:851	The diameter and sphericity of the beads prepared at pH = 6.0 proved more uniform and had a larger pore size with a good interconnectivity framework.
31835480	10	1	theme	tissue	1942:1947	arg1	engineering					1949:1959	tissue engineering	1942:1959	tissue engineering	1942:1959	Thus, this composition might have prospective as a functional material for bone and cartilage tissue engineering.
31835480	8	2	theme	polymer	1610:1616	arg1	chains					1618:1623	polymer chains	1610:1623	polymer chains	1610:1623	The in vitro kinetics of the release process was of the Peppas-Sahlin model, controlled by both diffusion and relaxation of polymer chains or swelling (anomalous transport mechanism).
31835480	6	3	theme	in	1273:1274	arg1	data					1295:1298	in vitro drug release data	1273:1298	in vitro drug release data	1273:1298	In in vitro drug release data, all beads presented a sustained release during the studied period (24 h).
31835480	8	4	theme	chains	1618:1623	arg1	swelling					1628:1635	swelling	1628:1635	swelling (anomalous transport mechanism)	1628:1667	The in vitro kinetics of the release process was of the Peppas-Sahlin model, controlled by both diffusion and relaxation of polymer chains or swelling (anomalous transport mechanism).
31835480	8	4	theme	chains	1618:1623	arg1	relaxation					1596:1605	relaxation	1596:1605	relaxation	1596:1605	The in vitro kinetics of the release process was of the Peppas-Sahlin model, controlled by both diffusion and relaxation of polymer chains or swelling (anomalous transport mechanism).
31835480	8	4	theme	chains	1618:1623	arg1	diffusion					1582:1590	diffusion	1582:1590	diffusion	1582:1590	The in vitro kinetics of the release process was of the Peppas-Sahlin model, controlled by both diffusion and relaxation of polymer chains or swelling (anomalous transport mechanism).
31835480	8	4	theme	chains	1618:1623	arg1	mechanism					1658:1666	anomalous transport mechanism	1638:1666	anomalous transport mechanism	1638:1666	The in vitro kinetics of the release process was of the Peppas-Sahlin model, controlled by both diffusion and relaxation of polymer chains or swelling (anomalous transport mechanism).
31835480	5	5	dep	pH	1131:1132	arg1	=					1134:1134	=	1134:1134	=	1134:1134	Attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) suggested a higher crosslinking degree for beads prepared at pH = 6.0, corroborated by X-ray diffraction profiles (XRD) analysis that indicated a decrease in the crystalline structure for such beads.
31835480	6	6	theme	studied	1352:1358	arg1	period					1360:1365	the studied period	1348:1365	the studied period (24 h)	1348:1372	In in vitro drug release data, all beads presented a sustained release during the studied period (24 h).
31835480	6	6	theme	studied	1352:1358	arg1	h					1371:1371	24 h	1368:1371	24 h	1368:1371	In in vitro drug release data, all beads presented a sustained release during the studied period (24 h).
31835480	1	7	theme	in	247:248	arg1	behavior					264:271	in vitro release behavior	247:271	in vitro release behavior	247:271	In this study, the effect of the ionic cross-linking mode on the ability to control physical properties and in vitro release behavior of the dexamethasone (DEX) drug from chitosan (CS) and chitosan/hydroxyapatite (CS/HA) beads was investigated.
31835480	4	8	theme	larger	932:937	arg1	size					944:947	a larger pore size	930:947	a larger pore size	930:947	The diameter and sphericity of the beads prepared at pH = 6.0 proved more uniform and had a larger pore size with a good interconnectivity framework.
31835480	3	9	theme	microscopy	613:622	arg1	results					668:674	Optical microscopy (OM) and scanning electron microscopy (SEM) results	605:674	Optical microscopy (OM) and scanning electron microscopy (SEM) results	605:674	Optical microscopy (OM) and scanning electron microscopy (SEM) results showed changes on the surface topology of the beads, with a reduction of roughness for beads prepared at pH = 6.0 and an increase for the one prepared at pH = 9.0.
31835480	3	10	theme	electron	642:649	arg1	SEM					663:665	SEM	663:665	SEM	663:665	Optical microscopy (OM) and scanning electron microscopy (SEM) results showed changes on the surface topology of the beads, with a reduction of roughness for beads prepared at pH = 6.0 and an increase for the one prepared at pH = 9.0.
31835480	3	10	theme	electron	642:649	arg1	microscopy					651:660	scanning electron microscopy	633:660	scanning electron microscopy (SEM)	633:666	Optical microscopy (OM) and scanning electron microscopy (SEM) results showed changes on the surface topology of the beads, with a reduction of roughness for beads prepared at pH = 6.0 and an increase for the one prepared at pH = 9.0.
31835480	8	11	theme	transport	1648:1656	arg1	swelling					1628:1635	swelling	1628:1635	swelling (anomalous transport mechanism)	1628:1667	The in vitro kinetics of the release process was of the Peppas-Sahlin model, controlled by both diffusion and relaxation of polymer chains or swelling (anomalous transport mechanism).
31835480	8	11	theme	transport	1648:1656	arg1	mechanism					1658:1666	anomalous transport mechanism	1638:1666	anomalous transport mechanism	1638:1666	The in vitro kinetics of the release process was of the Peppas-Sahlin model, controlled by both diffusion and relaxation of polymer chains or swelling (anomalous transport mechanism).
31835480	5	12	theme	crystalline	1232:1242	arg1	structure					1244:1252	the crystalline structure	1228:1252	the crystalline structure for such beads	1228:1267	Attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) suggested a higher crosslinking degree for beads prepared at pH = 6.0, corroborated by X-ray diffraction profiles (XRD) analysis that indicated a decrease in the crystalline structure for such beads.
31835480	6	13	theme	sustained	1323:1331	arg1	release					1333:1339	a sustained release	1321:1339	a sustained release during the studied period (24 h)	1321:1372	In in vitro drug release data, all beads presented a sustained release during the studied period (24 h).
31835480	2	14	theme	ionic	496:500	arg1	TPP					540:542	TPP	540:542	TPP	540:542	CS solutions without and with HA and DEX were dripped into two coagulation solutions, prepared with a non-toxic ionic crosslinker (sodium tripolyphosphate, TPP) and distilled water, one at pH = 9.0 and other at pH = 6.0.
31835480	2	14	theme	ionic	496:500	arg1	crosslinker					502:512	a non-toxic ionic crosslinker	484:512	a non-toxic ionic crosslinker (sodium tripolyphosphate, TPP)	484:543	CS solutions without and with HA and DEX were dripped into two coagulation solutions, prepared with a non-toxic ionic crosslinker (sodium tripolyphosphate, TPP) and distilled water, one at pH = 9.0 and other at pH = 6.0.
31835480	1	15	theme	cross-linking	178:190	arg1	mode					192:195	the ionic cross-linking mode	168:195	the ionic cross-linking mode	168:195	In this study, the effect of the ionic cross-linking mode on the ability to control physical properties and in vitro release behavior of the dexamethasone (DEX) drug from chitosan (CS) and chitosan/hydroxyapatite (CS/HA) beads was investigated.
31835480	9	16	theme	CS/HA	1706:1710	arg1	beads					1712:1716	DEX-loaded CS/HA beads	1695:1716	DEX-loaded CS/HA beads	1695:1716	Our results suggest that DEX-loaded CS/HA beads, crosslinked in TPP coagulation solution at pH = 9.0, led to a decrease in the DEX release rate and prolonged the release period.
31835480	1	17	from	properties	232:241	arg1	CS/HA					353:357	CS/HA	353:357	CS/HA	353:357	In this study, the effect of the ionic cross-linking mode on the ability to control physical properties and in vitro release behavior of the dexamethasone (DEX) drug from chitosan (CS) and chitosan/hydroxyapatite (CS/HA) beads was investigated.
31835480	1	17	from	properties	232:241	arg1	CS					320:321	CS	320:321	CS	320:321	In this study, the effect of the ionic cross-linking mode on the ability to control physical properties and in vitro release behavior of the dexamethasone (DEX) drug from chitosan (CS) and chitosan/hydroxyapatite (CS/HA) beads was investigated.
31835480	1	17	from	properties	232:241	arg1	chitosan/hydroxyapatite					328:350	chitosan/hydroxyapatite	328:350	chitosan/hydroxyapatite (CS/HA)	328:358	In this study, the effect of the ionic cross-linking mode on the ability to control physical properties and in vitro release behavior of the dexamethasone (DEX) drug from chitosan (CS) and chitosan/hydroxyapatite (CS/HA) beads was investigated.
31835480	1	17	from	properties	232:241	arg1	chitosan					310:317	chitosan	310:317	chitosan (CS)	310:322	In this study, the effect of the ionic cross-linking mode on the ability to control physical properties and in vitro release behavior of the dexamethasone (DEX) drug from chitosan (CS) and chitosan/hydroxyapatite (CS/HA) beads was investigated.
31835480	8	18	theme	release	1515:1521	arg1	process					1523:1529	the release process	1511:1529	the release process	1511:1529	The in vitro kinetics of the release process was of the Peppas-Sahlin model, controlled by both diffusion and relaxation of polymer chains or swelling (anomalous transport mechanism).
31835480	1	19	theme	release	256:262	arg1	behavior					264:271	in vitro release behavior	247:271	in vitro release behavior	247:271	In this study, the effect of the ionic cross-linking mode on the ability to control physical properties and in vitro release behavior of the dexamethasone (DEX) drug from chitosan (CS) and chitosan/hydroxyapatite (CS/HA) beads was investigated.
31835480	2	20	from	pH	573:574	arg1	solutions					459:467	two coagulation solutions	443:467	two coagulation solutions	443:467	CS solutions without and with HA and DEX were dripped into two coagulation solutions, prepared with a non-toxic ionic crosslinker (sodium tripolyphosphate, TPP) and distilled water, one at pH = 9.0 and other at pH = 6.0.
31835480	2	20	from	pH	573:574	arg1	one					566:568	one	566:568	one	566:568	CS solutions without and with HA and DEX were dripped into two coagulation solutions, prepared with a non-toxic ionic crosslinker (sodium tripolyphosphate, TPP) and distilled water, one at pH = 9.0 and other at pH = 6.0.
31835480	1	21	dep	in	247:248	arg1	vitro					250:254	vitro	250:254	vitro	250:254	In this study, the effect of the ionic cross-linking mode on the ability to control physical properties and in vitro release behavior of the dexamethasone (DEX) drug from chitosan (CS) and chitosan/hydroxyapatite (CS/HA) beads was investigated.
31835480	2	22	theme	=	598:598	arg1	pH					595:596	pH	595:596	pH = 6.0	595:602	CS solutions without and with HA and DEX were dripped into two coagulation solutions, prepared with a non-toxic ionic crosslinker (sodium tripolyphosphate, TPP) and distilled water, one at pH = 9.0 and other at pH = 6.0.
31835480	3	23	theme	beads	722:726	arg1	topology					706:713	the surface topology	694:713	the surface topology of the beads	694:726	Optical microscopy (OM) and scanning electron microscopy (SEM) results showed changes on the surface topology of the beads, with a reduction of roughness for beads prepared at pH = 6.0 and an increase for the one prepared at pH = 9.0.
31835480	7	24	theme	drug	1379:1382	arg1	rate					1392:1395	The drug release rate	1375:1395	The drug release rate	1375:1395	The drug release rate was affected by the pH of the coagulation solution used in the preparation of the beads.
31835480	9	25	theme	coagulation	1738:1748	arg1	solution					1750:1757	TPP coagulation solution	1734:1757	TPP coagulation solution	1734:1757	Our results suggest that DEX-loaded CS/HA beads, crosslinked in TPP coagulation solution at pH = 9.0, led to a decrease in the DEX release rate and prolonged the release period.
31835480	3	26	theme	roughness	749:757	arg1	increase					797:804	an increase	794:804	an increase for the one prepared at pH = 9.0	794:837	Optical microscopy (OM) and scanning electron microscopy (SEM) results showed changes on the surface topology of the beads, with a reduction of roughness for beads prepared at pH = 6.0 and an increase for the one prepared at pH = 9.0.
31835480	3	26	theme	roughness	749:757	arg1	reduction					736:744	a reduction	734:744	a reduction of roughness for beads prepared at pH = 6.0	734:788	Optical microscopy (OM) and scanning electron microscopy (SEM) results showed changes on the surface topology of the beads, with a reduction of roughness for beads prepared at pH = 6.0 and an increase for the one prepared at pH = 9.0.
31835480	5	27	dep	reflectance-Fourier	1007:1025	arg1	transform					1027:1035	transform	1027:1035	transform infrared spectroscopy (ATR-FTIR)	1027:1068	Attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) suggested a higher crosslinking degree for beads prepared at pH = 6.0, corroborated by X-ray diffraction profiles (XRD) analysis that indicated a decrease in the crystalline structure for such beads.
31835480	4	28	theme	interconnectivity	961:977	arg1	framework					979:987	a good interconnectivity framework	954:987	a good interconnectivity framework	954:987	The diameter and sphericity of the beads prepared at pH = 6.0 proved more uniform and had a larger pore size with a good interconnectivity framework.
31835480	2	29	from	pH	595:596	arg1	other					586:590	other	586:590	other	586:590	CS solutions without and with HA and DEX were dripped into two coagulation solutions, prepared with a non-toxic ionic crosslinker (sodium tripolyphosphate, TPP) and distilled water, one at pH = 9.0 and other at pH = 6.0.
31835480	2	29	from	pH	595:596	arg1	solutions					459:467	two coagulation solutions	443:467	two coagulation solutions	443:467	CS solutions without and with HA and DEX were dripped into two coagulation solutions, prepared with a non-toxic ionic crosslinker (sodium tripolyphosphate, TPP) and distilled water, one at pH = 9.0 and other at pH = 6.0.
31835480	5	30	theme	crosslinking	1089:1100	arg1	degree					1102:1107	a higher crosslinking degree	1080:1107	a higher crosslinking degree for beads prepared at pH = 6.0, corroborated by X-ray diffraction profiles (XRD) analysis that indicated a decrease in the crystalline structure for such beads	1080:1267	Attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) suggested a higher crosslinking degree for beads prepared at pH = 6.0, corroborated by X-ray diffraction profiles (XRD) analysis that indicated a decrease in the crystalline structure for such beads.
31835480	2	31	theme	=	576:576	arg1	pH					573:574	pH	573:574	pH = 9.0	573:580	CS solutions without and with HA and DEX were dripped into two coagulation solutions, prepared with a non-toxic ionic crosslinker (sodium tripolyphosphate, TPP) and distilled water, one at pH = 9.0 and other at pH = 6.0.
31835480	3	32	theme	surface	698:704	arg1	topology					706:713	the surface topology	694:713	the surface topology of the beads	694:726	Optical microscopy (OM) and scanning electron microscopy (SEM) results showed changes on the surface topology of the beads, with a reduction of roughness for beads prepared at pH = 6.0 and an increase for the one prepared at pH = 9.0.
31835480	9	33	theme	TPP	1734:1736	arg1	solution					1750:1757	TPP coagulation solution	1734:1757	TPP coagulation solution	1734:1757	Our results suggest that DEX-loaded CS/HA beads, crosslinked in TPP coagulation solution at pH = 9.0, led to a decrease in the DEX release rate and prolonged the release period.
31835480	8	34	dep	in	1490:1491	arg1	vitro					1493:1497	vitro	1493:1497	vitro	1493:1497	The in vitro kinetics of the release process was of the Peppas-Sahlin model, controlled by both diffusion and relaxation of polymer chains or swelling (anomalous transport mechanism).
31835480	5	35	theme	X-ray	1157:1161	arg1	XRD					1185:1187	XRD	1185:1187	XRD	1185:1187	Attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) suggested a higher crosslinking degree for beads prepared at pH = 6.0, corroborated by X-ray diffraction profiles (XRD) analysis that indicated a decrease in the crystalline structure for such beads.
31835480	5	35	theme	X-ray	1157:1161	arg1	profiles					1175:1182	X-ray diffraction profiles	1157:1182	X-ray diffraction profiles (XRD) analysis that indicated a decrease in the crystalline structure for such beads	1157:1267	Attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) suggested a higher crosslinking degree for beads prepared at pH = 6.0, corroborated by X-ray diffraction profiles (XRD) analysis that indicated a decrease in the crystalline structure for such beads.
31835480	0	36	theme	Release	86:92	arg1	Properties					94:103	the Physical and In Vitro Dexamethasone Release Properties	46:103	the Physical and In Vitro Dexamethasone Release Properties of Chitosan/Hydroxyapatite Beads	46:136	The Impact of the Ionic Cross-Linking Mode on the Physical and In Vitro Dexamethasone Release Properties of Chitosan/Hydroxyapatite Beads.
31835480	10	37	dep	bone	1923:1926	arg1	engineering					1949:1959	tissue engineering	1942:1959	tissue engineering	1942:1959	Thus, this composition might have prospective as a functional material for bone and cartilage tissue engineering.
31835480	5	38	theme	profiles	1175:1182	arg1	analysis					1190:1197	X-ray diffraction profiles (XRD) analysis	1157:1197	X-ray diffraction profiles (XRD) analysis that indicated a decrease in the crystalline structure for such beads	1157:1267	Attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) suggested a higher crosslinking degree for beads prepared at pH = 6.0, corroborated by X-ray diffraction profiles (XRD) analysis that indicated a decrease in the crystalline structure for such beads.
31835480	8	39	theme	anomalous	1638:1646	arg1	swelling					1628:1635	swelling	1628:1635	swelling (anomalous transport mechanism)	1628:1667	The in vitro kinetics of the release process was of the Peppas-Sahlin model, controlled by both diffusion and relaxation of polymer chains or swelling (anomalous transport mechanism).
31835480	8	39	theme	anomalous	1638:1646	arg1	mechanism					1658:1666	anomalous transport mechanism	1638:1666	anomalous transport mechanism	1638:1666	The in vitro kinetics of the release process was of the Peppas-Sahlin model, controlled by both diffusion and relaxation of polymer chains or swelling (anomalous transport mechanism).
31835480	7	40	theme	solution	1439:1446	arg1	pH					1417:1418	the pH	1413:1418	the pH of the coagulation solution used in the preparation of the beads	1413:1483	The drug release rate was affected by the pH of the coagulation solution used in the preparation of the beads.
31835480	1	41	dep	chitosan	310:317	arg1	beads					360:364	beads	360:364	beads	360:364	In this study, the effect of the ionic cross-linking mode on the ability to control physical properties and in vitro release behavior of the dexamethasone (DEX) drug from chitosan (CS) and chitosan/hydroxyapatite (CS/HA) beads was investigated.
31835480	5	42	theme	total	1001:1005	arg1	reflectance-Fourier					1007:1025	Attenuated total reflectance-Fourier	990:1025	Attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR)	990:1068	Attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) suggested a higher crosslinking degree for beads prepared at pH = 6.0, corroborated by X-ray diffraction profiles (XRD) analysis that indicated a decrease in the crystalline structure for such beads.
31835480	5	43	from	decrease	1216:1223	arg1	structure					1244:1252	the crystalline structure	1228:1252	the crystalline structure for such beads	1228:1267	Attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) suggested a higher crosslinking degree for beads prepared at pH = 6.0, corroborated by X-ray diffraction profiles (XRD) analysis that indicated a decrease in the crystalline structure for such beads.
31835480	0	44	theme	Ionic	18:22	arg1	Mode					38:41	the Ionic Cross-Linking Mode	14:41	the Ionic Cross-Linking Mode	14:41	The Impact of the Ionic Cross-Linking Mode on the Physical and In Vitro Dexamethasone Release Properties of Chitosan/Hydroxyapatite Beads.
31835480	1	45	from	chitosan	310:317	arg1	behavior					264:271	in vitro release behavior	247:271	in vitro release behavior	247:271	In this study, the effect of the ionic cross-linking mode on the ability to control physical properties and in vitro release behavior of the dexamethasone (DEX) drug from chitosan (CS) and chitosan/hydroxyapatite (CS/HA) beads was investigated.
31835480	1	45	from	chitosan	310:317	arg1	properties					232:241	physical properties	223:241	physical properties	223:241	In this study, the effect of the ionic cross-linking mode on the ability to control physical properties and in vitro release behavior of the dexamethasone (DEX) drug from chitosan (CS) and chitosan/hydroxyapatite (CS/HA) beads was investigated.
31835480	1	45	from	chitosan	310:317	arg1	drug					300:303	the dexamethasone (DEX) drug	276:303	the dexamethasone (DEX) drug from chitosan (CS) and chitosan/hydroxyapatite (CS/HA) beads	276:364	In this study, the effect of the ionic cross-linking mode on the ability to control physical properties and in vitro release behavior of the dexamethasone (DEX) drug from chitosan (CS) and chitosan/hydroxyapatite (CS/HA) beads was investigated.
31835480	6	46	dep	in	1273:1274	arg1	vitro					1276:1280	vitro	1276:1280	vitro	1276:1280	In in vitro drug release data, all beads presented a sustained release during the studied period (24 h).
31835480	10	47	theme	functional	1899:1908	arg1	material					1910:1917	a functional material	1897:1917	a functional material for bone and cartilage tissue engineering	1897:1959	Thus, this composition might have prospective as a functional material for bone and cartilage tissue engineering.
31835480	9	48	theme	release	1801:1807	arg1	rate					1809:1812	the DEX release rate	1793:1812	the DEX release rate	1793:1812	Our results suggest that DEX-loaded CS/HA beads, crosslinked in TPP coagulation solution at pH = 9.0, led to a decrease in the DEX release rate and prolonged the release period.
31835480	0	49	theme	Mode	38:41	arg1	Impact					4:9	The Impact	0:9	The Impact of the Ionic Cross-Linking Mode on the Physical and In Vitro Dexamethasone Release Properties of Chitosan/Hydroxyapatite Beads.	0:137	The Impact of the Ionic Cross-Linking Mode on the Physical and In Vitro Dexamethasone Release Properties of Chitosan/Hydroxyapatite Beads.
31835480	9	50	from	decrease	1781:1788	arg1	rate					1809:1812	the DEX release rate	1793:1812	the DEX release rate	1793:1812	Our results suggest that DEX-loaded CS/HA beads, crosslinked in TPP coagulation solution at pH = 9.0, led to a decrease in the DEX release rate and prolonged the release period.
31835480	6	51	theme	release	1287:1293	arg1	data					1295:1298	in vitro drug release data	1273:1298	in vitro drug release data	1273:1298	In in vitro drug release data, all beads presented a sustained release during the studied period (24 h).
31835480	9	52	dep	=	1765:1765	arg1	9.0					1767:1769	9.0	1767:1769	9.0	1767:1769	Our results suggest that DEX-loaded CS/HA beads, crosslinked in TPP coagulation solution at pH = 9.0, led to a decrease in the DEX release rate and prolonged the release period.
31835480	3	53	theme	Optical	605:611	arg1	OM					625:626	OM	625:626	OM	625:626	Optical microscopy (OM) and scanning electron microscopy (SEM) results showed changes on the surface topology of the beads, with a reduction of roughness for beads prepared at pH = 6.0 and an increase for the one prepared at pH = 9.0.
31835480	3	53	theme	Optical	605:611	arg1	microscopy					613:622	Optical microscopy	605:622	Optical microscopy (OM)	605:627	Optical microscopy (OM) and scanning electron microscopy (SEM) results showed changes on the surface topology of the beads, with a reduction of roughness for beads prepared at pH = 6.0 and an increase for the one prepared at pH = 9.0.
31835480	4	54	dep	diameter	844:851	arg1	The					840:842	The	840:842	The	840:842	The diameter and sphericity of the beads prepared at pH = 6.0 proved more uniform and had a larger pore size with a good interconnectivity framework.
31835480	1	55	theme	physical	223:230	arg1	properties					232:241	physical properties	223:241	physical properties	223:241	In this study, the effect of the ionic cross-linking mode on the ability to control physical properties and in vitro release behavior of the dexamethasone (DEX) drug from chitosan (CS) and chitosan/hydroxyapatite (CS/HA) beads was investigated.
31835480	5	56	theme	such	1258:1261	arg1	beads					1263:1267	such beads	1258:1267	such beads	1258:1267	Attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) suggested a higher crosslinking degree for beads prepared at pH = 6.0, corroborated by X-ray diffraction profiles (XRD) analysis that indicated a decrease in the crystalline structure for such beads.
31835480	8	57	theme	Peppas-Sahlin	1542:1554	arg1	model					1556:1560	the Peppas-Sahlin model	1538:1560	the Peppas-Sahlin model	1538:1560	The in vitro kinetics of the release process was of the Peppas-Sahlin model, controlled by both diffusion and relaxation of polymer chains or swelling (anomalous transport mechanism).
31835480	6	58	attach	presented	1311:1319	arg2	beads					1305:1309	all beads	1301:1309	all beads	1301:1309	In in vitro drug release data, all beads presented a sustained release during the studied period (24 h).
31835480	6	58	attach	presented	1311:1319	arg1	data					1295:1298	in vitro drug release data	1273:1298	in vitro drug release data	1273:1298	In in vitro drug release data, all beads presented a sustained release during the studied period (24 h).
31835480	4	59	theme	pore	939:942	arg1	size					944:947	a larger pore size	930:947	a larger pore size	930:947	The diameter and sphericity of the beads prepared at pH = 6.0 proved more uniform and had a larger pore size with a good interconnectivity framework.
31835480	1	60	from	behavior	264:271	arg1	CS/HA					353:357	CS/HA	353:357	CS/HA	353:357	In this study, the effect of the ionic cross-linking mode on the ability to control physical properties and in vitro release behavior of the dexamethasone (DEX) drug from chitosan (CS) and chitosan/hydroxyapatite (CS/HA) beads was investigated.
31835480	1	60	from	behavior	264:271	arg1	CS					320:321	CS	320:321	CS	320:321	In this study, the effect of the ionic cross-linking mode on the ability to control physical properties and in vitro release behavior of the dexamethasone (DEX) drug from chitosan (CS) and chitosan/hydroxyapatite (CS/HA) beads was investigated.
31835480	1	60	from	behavior	264:271	arg1	chitosan/hydroxyapatite					328:350	chitosan/hydroxyapatite	328:350	chitosan/hydroxyapatite (CS/HA)	328:358	In this study, the effect of the ionic cross-linking mode on the ability to control physical properties and in vitro release behavior of the dexamethasone (DEX) drug from chitosan (CS) and chitosan/hydroxyapatite (CS/HA) beads was investigated.
31835480	1	60	from	behavior	264:271	arg1	chitosan					310:317	chitosan	310:317	chitosan (CS)	310:322	In this study, the effect of the ionic cross-linking mode on the ability to control physical properties and in vitro release behavior of the dexamethasone (DEX) drug from chitosan (CS) and chitosan/hydroxyapatite (CS/HA) beads was investigated.
31835480	2	61	theme	distilled	549:557	arg1	water					559:563	distilled water	549:563	distilled water	549:563	CS solutions without and with HA and DEX were dripped into two coagulation solutions, prepared with a non-toxic ionic crosslinker (sodium tripolyphosphate, TPP) and distilled water, one at pH = 9.0 and other at pH = 6.0.
31835480	3	62	theme	microscopy	651:660	arg1	results					668:674	Optical microscopy (OM) and scanning electron microscopy (SEM) results	605:674	Optical microscopy (OM) and scanning electron microscopy (SEM) results	605:674	Optical microscopy (OM) and scanning electron microscopy (SEM) results showed changes on the surface topology of the beads, with a reduction of roughness for beads prepared at pH = 6.0 and an increase for the one prepared at pH = 9.0.
31835480	0	63	theme	Chitosan/Hydroxyapatite	108:130	arg1	Beads					132:136	Chitosan/Hydroxyapatite Beads	108:136	Chitosan/Hydroxyapatite Beads	108:136	The Impact of the Ionic Cross-Linking Mode on the Physical and In Vitro Dexamethasone Release Properties of Chitosan/Hydroxyapatite Beads.
31835480	8	64	theme	in	1490:1491	arg1	kinetics					1499:1506	The in vitro kinetics	1486:1506	The in vitro kinetics of the release process	1486:1529	The in vitro kinetics of the release process was of the Peppas-Sahlin model, controlled by both diffusion and relaxation of polymer chains or swelling (anomalous transport mechanism).
31835480	1	65	theme	ionic	172:176	arg1	mode					192:195	the ionic cross-linking mode	168:195	the ionic cross-linking mode	168:195	In this study, the effect of the ionic cross-linking mode on the ability to control physical properties and in vitro release behavior of the dexamethasone (DEX) drug from chitosan (CS) and chitosan/hydroxyapatite (CS/HA) beads was investigated.
31835480	2	66	theme	non-toxic	486:494	arg1	TPP					540:542	TPP	540:542	TPP	540:542	CS solutions without and with HA and DEX were dripped into two coagulation solutions, prepared with a non-toxic ionic crosslinker (sodium tripolyphosphate, TPP) and distilled water, one at pH = 9.0 and other at pH = 6.0.
31835480	2	66	theme	non-toxic	486:494	arg1	crosslinker					502:512	a non-toxic ionic crosslinker	484:512	a non-toxic ionic crosslinker (sodium tripolyphosphate, TPP)	484:543	CS solutions without and with HA and DEX were dripped into two coagulation solutions, prepared with a non-toxic ionic crosslinker (sodium tripolyphosphate, TPP) and distilled water, one at pH = 9.0 and other at pH = 6.0.
31835480	9	67	theme	DEX-loaded	1695:1704	arg1	beads					1712:1716	DEX-loaded CS/HA beads	1695:1716	DEX-loaded CS/HA beads	1695:1716	Our results suggest that DEX-loaded CS/HA beads, crosslinked in TPP coagulation solution at pH = 9.0, led to a decrease in the DEX release rate and prolonged the release period.
31835480	3	68	theme	scanning	633:640	arg1	SEM					663:665	SEM	663:665	SEM	663:665	Optical microscopy (OM) and scanning electron microscopy (SEM) results showed changes on the surface topology of the beads, with a reduction of roughness for beads prepared at pH = 6.0 and an increase for the one prepared at pH = 9.0.
31835480	3	68	theme	scanning	633:640	arg1	microscopy					651:660	scanning electron microscopy	633:660	scanning electron microscopy (SEM)	633:666	Optical microscopy (OM) and scanning electron microscopy (SEM) results showed changes on the surface topology of the beads, with a reduction of roughness for beads prepared at pH = 6.0 and an increase for the one prepared at pH = 9.0.
31835480	3	69	dep	=	833:833	arg1	9.0					835:837	9.0	835:837	9.0	835:837	Optical microscopy (OM) and scanning electron microscopy (SEM) results showed changes on the surface topology of the beads, with a reduction of roughness for beads prepared at pH = 6.0 and an increase for the one prepared at pH = 9.0.
31835480	1	70	theme	mode	192:195	arg1	effect					158:163	the effect	154:163	the effect of the ionic cross-linking mode on the ability to control physical properties and in vitro release behavior of the dexamethasone (DEX) drug from chitosan (CS) and chitosan/hydroxyapatite (CS/HA) beads	154:364	In this study, the effect of the ionic cross-linking mode on the ability to control physical properties and in vitro release behavior of the dexamethasone (DEX) drug from chitosan (CS) and chitosan/hydroxyapatite (CS/HA) beads was investigated.
31835480	7	71	theme	release	1384:1390	arg1	rate					1392:1395	The drug release rate	1375:1395	The drug release rate	1375:1395	The drug release rate was affected by the pH of the coagulation solution used in the preparation of the beads.
31835480	5	72	dep	spectroscopy	1046:1057	arg1	ATR-FTIR					1060:1067	ATR-FTIR	1060:1067	ATR-FTIR	1060:1067	Attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) suggested a higher crosslinking degree for beads prepared at pH = 6.0, corroborated by X-ray diffraction profiles (XRD) analysis that indicated a decrease in the crystalline structure for such beads.
31835480	8	73	theme	process	1523:1529	arg1	kinetics					1499:1506	The in vitro kinetics	1486:1506	The in vitro kinetics of the release process	1486:1529	The in vitro kinetics of the release process was of the Peppas-Sahlin model, controlled by both diffusion and relaxation of polymer chains or swelling (anomalous transport mechanism).
31835480	9	74	theme	=	1765:1765	arg1	pH					1762:1763	pH	1762:1763	pH = 9.0	1762:1769	Our results suggest that DEX-loaded CS/HA beads, crosslinked in TPP coagulation solution at pH = 9.0, led to a decrease in the DEX release rate and prolonged the release period.
31835480	4	75	theme	good	956:959	arg1	framework					979:987	a good interconnectivity framework	954:987	a good interconnectivity framework	954:987	The diameter and sphericity of the beads prepared at pH = 6.0 proved more uniform and had a larger pore size with a good interconnectivity framework.
31835480	0	76	theme	Physical	50:57	arg1	Properties					94:103	the Physical and In Vitro Dexamethasone Release Properties	46:103	the Physical and In Vitro Dexamethasone Release Properties of Chitosan/Hydroxyapatite Beads	46:136	The Impact of the Ionic Cross-Linking Mode on the Physical and In Vitro Dexamethasone Release Properties of Chitosan/Hydroxyapatite Beads.
31835480	7	77	theme	beads	1479:1483	arg1	preparation					1460:1470	the preparation	1456:1470	the preparation of the beads	1456:1483	The drug release rate was affected by the pH of the coagulation solution used in the preparation of the beads.
31835480	5	78	theme	higher	1082:1087	arg1	degree					1102:1107	a higher crosslinking degree	1080:1107	a higher crosslinking degree for beads prepared at pH = 6.0, corroborated by X-ray diffraction profiles (XRD) analysis that indicated a decrease in the crystalline structure for such beads	1080:1267	Attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) suggested a higher crosslinking degree for beads prepared at pH = 6.0, corroborated by X-ray diffraction profiles (XRD) analysis that indicated a decrease in the crystalline structure for such beads.
31835480	0	79	theme	In	63:64	arg1	Properties					94:103	the Physical and In Vitro Dexamethasone Release Properties	46:103	the Physical and In Vitro Dexamethasone Release Properties of Chitosan/Hydroxyapatite Beads	46:136	The Impact of the Ionic Cross-Linking Mode on the Physical and In Vitro Dexamethasone Release Properties of Chitosan/Hydroxyapatite Beads.
31835480	1	80	theme	drug	300:303	arg1	behavior					264:271	in vitro release behavior	247:271	in vitro release behavior	247:271	In this study, the effect of the ionic cross-linking mode on the ability to control physical properties and in vitro release behavior of the dexamethasone (DEX) drug from chitosan (CS) and chitosan/hydroxyapatite (CS/HA) beads was investigated.
31835480	1	80	theme	drug	300:303	arg1	properties					232:241	physical properties	223:241	physical properties	223:241	In this study, the effect of the ionic cross-linking mode on the ability to control physical properties and in vitro release behavior of the dexamethasone (DEX) drug from chitosan (CS) and chitosan/hydroxyapatite (CS/HA) beads was investigated.
31835480	1	81	from	effect	158:163	arg1	ability					204:210	the ability to control physical properties and in vitro release behavior of the dexamethasone (DEX) drug from chitosan (CS) and chitosan/hydroxyapatite (CS/HA) beads	200:364	the ability to control physical properties and in vitro release behavior of the dexamethasone (DEX) drug from chitosan (CS) and chitosan/hydroxyapatite (CS/HA) beads	200:364	In this study, the effect of the ionic cross-linking mode on the ability to control physical properties and in vitro release behavior of the dexamethasone (DEX) drug from chitosan (CS) and chitosan/hydroxyapatite (CS/HA) beads was investigated.
31835480	0	82	from	Impact	4:9	arg1	Properties					94:103	the Physical and In Vitro Dexamethasone Release Properties	46:103	the Physical and In Vitro Dexamethasone Release Properties of Chitosan/Hydroxyapatite Beads	46:136	The Impact of the Ionic Cross-Linking Mode on the Physical and In Vitro Dexamethasone Release Properties of Chitosan/Hydroxyapatite Beads.
31835480	0	83	theme	Dexamethasone	72:84	arg1	Properties					94:103	the Physical and In Vitro Dexamethasone Release Properties	46:103	the Physical and In Vitro Dexamethasone Release Properties of Chitosan/Hydroxyapatite Beads	46:136	The Impact of the Ionic Cross-Linking Mode on the Physical and In Vitro Dexamethasone Release Properties of Chitosan/Hydroxyapatite Beads.
31835480	2	84	dep	=	576:576	arg1	9.0					578:580	9.0	578:580	9.0	578:580	CS solutions without and with HA and DEX were dripped into two coagulation solutions, prepared with a non-toxic ionic crosslinker (sodium tripolyphosphate, TPP) and distilled water, one at pH = 9.0 and other at pH = 6.0.
31835480	1	85	from	chitosan/hydroxyapatite	328:350	arg1	behavior					264:271	in vitro release behavior	247:271	in vitro release behavior	247:271	In this study, the effect of the ionic cross-linking mode on the ability to control physical properties and in vitro release behavior of the dexamethasone (DEX) drug from chitosan (CS) and chitosan/hydroxyapatite (CS/HA) beads was investigated.
31835480	1	85	from	chitosan/hydroxyapatite	328:350	arg1	properties					232:241	physical properties	223:241	physical properties	223:241	In this study, the effect of the ionic cross-linking mode on the ability to control physical properties and in vitro release behavior of the dexamethasone (DEX) drug from chitosan (CS) and chitosan/hydroxyapatite (CS/HA) beads was investigated.
31835480	1	85	from	chitosan/hydroxyapatite	328:350	arg1	drug					300:303	the dexamethasone (DEX) drug	276:303	the dexamethasone (DEX) drug from chitosan (CS) and chitosan/hydroxyapatite (CS/HA) beads	276:364	In this study, the effect of the ionic cross-linking mode on the ability to control physical properties and in vitro release behavior of the dexamethasone (DEX) drug from chitosan (CS) and chitosan/hydroxyapatite (CS/HA) beads was investigated.
31835480	2	86	theme	coagulation	447:457	arg1	solutions					459:467	two coagulation solutions	443:467	two coagulation solutions	443:467	CS solutions without and with HA and DEX were dripped into two coagulation solutions, prepared with a non-toxic ionic crosslinker (sodium tripolyphosphate, TPP) and distilled water, one at pH = 9.0 and other at pH = 6.0.
31835480	2	86	theme	coagulation	447:457	arg1	other					586:590	other	586:590	other	586:590	CS solutions without and with HA and DEX were dripped into two coagulation solutions, prepared with a non-toxic ionic crosslinker (sodium tripolyphosphate, TPP) and distilled water, one at pH = 9.0 and other at pH = 6.0.
31835480	2	86	theme	coagulation	447:457	arg1	one					566:568	one	566:568	one	566:568	CS solutions without and with HA and DEX were dripped into two coagulation solutions, prepared with a non-toxic ionic crosslinker (sodium tripolyphosphate, TPP) and distilled water, one at pH = 9.0 and other at pH = 6.0.
31835480	5	87	theme	diffraction	1163:1173	arg1	XRD					1185:1187	XRD	1185:1187	XRD	1185:1187	Attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) suggested a higher crosslinking degree for beads prepared at pH = 6.0, corroborated by X-ray diffraction profiles (XRD) analysis that indicated a decrease in the crystalline structure for such beads.
31835480	5	87	theme	diffraction	1163:1173	arg1	profiles					1175:1182	X-ray diffraction profiles	1157:1182	X-ray diffraction profiles (XRD) analysis that indicated a decrease in the crystalline structure for such beads	1157:1267	Attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) suggested a higher crosslinking degree for beads prepared at pH = 6.0, corroborated by X-ray diffraction profiles (XRD) analysis that indicated a decrease in the crystalline structure for such beads.
31835480	9	88	attach	crosslinked	1719:1729	arg3	pH					1762:1763	pH	1762:1763	pH = 9.0	1762:1769	Our results suggest that DEX-loaded CS/HA beads, crosslinked in TPP coagulation solution at pH = 9.0, led to a decrease in the DEX release rate and prolonged the release period.
31835480	9	88	attach	crosslinked	1719:1729	arg3	solution					1750:1757	TPP coagulation solution	1734:1757	TPP coagulation solution	1734:1757	Our results suggest that DEX-loaded CS/HA beads, crosslinked in TPP coagulation solution at pH = 9.0, led to a decrease in the DEX release rate and prolonged the release period.
31835480	0	89	dep	In	63:64	arg1	Vitro					66:70	Vitro	66:70	Vitro	66:70	The Impact of the Ionic Cross-Linking Mode on the Physical and In Vitro Dexamethasone Release Properties of Chitosan/Hydroxyapatite Beads.
31835480	5	90	theme	Attenuated	990:999	arg1	reflectance-Fourier					1007:1025	Attenuated total reflectance-Fourier	990:1025	Attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR)	990:1068	Attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) suggested a higher crosslinking degree for beads prepared at pH = 6.0, corroborated by X-ray diffraction profiles (XRD) analysis that indicated a decrease in the crystalline structure for such beads.
31835480	4	91	contain	had	926:928	arg2	size					944:947	a larger pore size	930:947	a larger pore size	930:947	The diameter and sphericity of the beads prepared at pH = 6.0 proved more uniform and had a larger pore size with a good interconnectivity framework.
31835480	4	91	contain	had	926:928	arg1	diameter					844:851	diameter	844:851	diameter	844:851	The diameter and sphericity of the beads prepared at pH = 6.0 proved more uniform and had a larger pore size with a good interconnectivity framework.
31835480	2	92	dep	=	598:598	arg1	6.0					600:602	6.0	600:602	6.0	600:602	CS solutions without and with HA and DEX were dripped into two coagulation solutions, prepared with a non-toxic ionic crosslinker (sodium tripolyphosphate, TPP) and distilled water, one at pH = 9.0 and other at pH = 6.0.
31835480	2	93	theme	CS	384:385	arg1	solutions					387:395	CS solutions	384:395	CS solutions without and with HA and DEX	384:423	CS solutions without and with HA and DEX were dripped into two coagulation solutions, prepared with a non-toxic ionic crosslinker (sodium tripolyphosphate, TPP) and distilled water, one at pH = 9.0 and other at pH = 6.0.
31835480	0	94	theme	Cross-Linking	24:36	arg1	Mode					38:41	the Ionic Cross-Linking Mode	14:41	the Ionic Cross-Linking Mode	14:41	The Impact of the Ionic Cross-Linking Mode on the Physical and In Vitro Dexamethasone Release Properties of Chitosan/Hydroxyapatite Beads.
31835480	7	95	theme	coagulation	1427:1437	arg1	solution					1439:1446	the coagulation solution	1423:1446	the coagulation solution used in the preparation of the beads	1423:1483	The drug release rate was affected by the pH of the coagulation solution used in the preparation of the beads.
31835480	5	96	theme	infrared	1037:1044	arg1	spectroscopy					1046:1057	infrared spectroscopy	1037:1057	infrared spectroscopy (ATR-FTIR)	1037:1068	Attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) suggested a higher crosslinking degree for beads prepared at pH = 6.0, corroborated by X-ray diffraction profiles (XRD) analysis that indicated a decrease in the crystalline structure for such beads.
31835480	9	97	theme	DEX	1797:1799	arg1	rate					1809:1812	the DEX release rate	1793:1812	the DEX release rate	1793:1812	Our results suggest that DEX-loaded CS/HA beads, crosslinked in TPP coagulation solution at pH = 9.0, led to a decrease in the DEX release rate and prolonged the release period.
31835480	1	98	theme	dexamethasone	280:292	arg1	drug					300:303	the dexamethasone (DEX) drug	276:303	the dexamethasone (DEX) drug from chitosan (CS) and chitosan/hydroxyapatite (CS/HA) beads	276:364	In this study, the effect of the ionic cross-linking mode on the ability to control physical properties and in vitro release behavior of the dexamethasone (DEX) drug from chitosan (CS) and chitosan/hydroxyapatite (CS/HA) beads was investigated.
31835480	0	99	theme	Beads	132:136	arg1	Properties					94:103	the Physical and In Vitro Dexamethasone Release Properties	46:103	the Physical and In Vitro Dexamethasone Release Properties of Chitosan/Hydroxyapatite Beads	46:136	The Impact of the Ionic Cross-Linking Mode on the Physical and In Vitro Dexamethasone Release Properties of Chitosan/Hydroxyapatite Beads.
31835480	3	100	from	changes	683:689	arg1	topology					706:713	the surface topology	694:713	the surface topology of the beads	694:726	Optical microscopy (OM) and scanning electron microscopy (SEM) results showed changes on the surface topology of the beads, with a reduction of roughness for beads prepared at pH = 6.0 and an increase for the one prepared at pH = 9.0.
31835480	3	101	theme	=	833:833	arg1	pH					830:831	pH	830:831	pH = 9.0	830:837	Optical microscopy (OM) and scanning electron microscopy (SEM) results showed changes on the surface topology of the beads, with a reduction of roughness for beads prepared at pH = 6.0 and an increase for the one prepared at pH = 9.0.
31835480	9	102	theme	release	1832:1838	arg1	period					1840:1845	the release period	1828:1845	the release period	1828:1845	Our results suggest that DEX-loaded CS/HA beads, crosslinked in TPP coagulation solution at pH = 9.0, led to a decrease in the DEX release rate and prolonged the release period.
31835480	6	103	theme	drug	1282:1285	arg1	data					1295:1298	in vitro drug release data	1273:1298	in vitro drug release data	1273:1298	In in vitro drug release data, all beads presented a sustained release during the studied period (24 h).
30316599	7	0	theme	low-starch	1724:1733	arg1	diets					1735:1739	high-fiber, low-starch diets	1712:1739	diets	1735:1739	Rumination displayed a treatment by time interaction for both NDF and starch concentration, with high-fiber, low-starch diets causing greater rumination overnight but not midday.
30316599	4	1	dep	diets	1145:1149	arg1	%					1161:1161	21 and 32%	1152:1161	21 and 32%	1152:1161	To investigate starch source and concentration in the second experiment, ground high-moisture corn or dry ground corn were fed in low- and high-starch diets (21 and 32%, respectively).
30316599	2	2	theme	acids	504:508	arg1	influence					458:466	the influence	454:466	the influence of dietary starch, fiber, and fatty acids (FA) on daily patterns of intake, rumination, and rumen pH. Dietary neutral detergent fiber (NDF) and starch were investigated in 2 experiments, each with duplicated 4 × 4 Latin square designs with a 2 × 2 factorial arrangement of treatments in cows fed cows 1×/d at 1200 and 1400 h, respectively	454:805	Data from 3 previously published experiments were examined to determine the influence of dietary starch, fiber, and fatty acids (FA) on daily patterns of intake, rumination, and rumen pH. Dietary neutral detergent fiber (NDF) and starch were investigated in 2 experiments, each with duplicated 4 × 4 Latin square designs with a 2 × 2 factorial arrangement of treatments in cows fed cows 1×/d at 1200 and 1400 h, respectively.
30316599	0	3	from	rumination	120:129	arg1	cows					154:157	dairy cows	148:157	dairy cows	148:157	The effects of source and concentration of dietary fiber, starch, and fatty acids on the daily patterns of feed intake, rumination, and rumen pH in dairy cows.
30316599	4	4	theme	low-	1124:1127	arg1	diets					1145:1149	low- and high-starch diets	1124:1149	low- and high-starch diets (21 and 32%, respectively)	1124:1176	To investigate starch source and concentration in the second experiment, ground high-moisture corn or dry ground corn were fed in low- and high-starch diets (21 and 32%, respectively).
30316599	9	5	from	day	2020:2022	arg1	diets					2040:2044	brown midrib diets	2027:2044	brown midrib diets	2027:2044	Type of corn silage did not affect the daily patterns of rumination or rumen pH, but pH was reduced throughout the day in brown midrib diets.
30316599	1	6	theme	intake	187:192	arg1	patterns					170:177	The daily patterns	160:177	The daily patterns of feed intake and rumination	160:207	The daily patterns of feed intake and rumination influence rumen fermentation, rumen pH, and timing of absorbed nutrients in the dairy cow, but the effects of diet composition on these patterns are not well characterized.
30316599	7	7	theme	high-fiber	1712:1721	arg1	diets					1735:1739	high-fiber, low-starch diets	1712:1739	diets	1735:1739	Rumination displayed a treatment by time interaction for both NDF and starch concentration, with high-fiber, low-starch diets causing greater rumination overnight but not midday.
30316599	0	8	from	intake	112:117	arg1	cows					154:157	dairy cows	148:157	dairy cows	148:157	The effects of source and concentration of dietary fiber, starch, and fatty acids on the daily patterns of feed intake, rumination, and rumen pH in dairy cows.
30316599	4	9	theme	dry	1096:1098	arg1	corn					1107:1110	dry ground corn	1096:1110	dry ground corn	1096:1110	To investigate starch source and concentration in the second experiment, ground high-moisture corn or dry ground corn were fed in low- and high-starch diets (21 and 32%, respectively).
30316599	1	10	from	pH	245:246	arg1	cow					295:297	the dairy cow	285:297	the dairy cow	285:297	The daily patterns of feed intake and rumination influence rumen fermentation, rumen pH, and timing of absorbed nutrients in the dairy cow, but the effects of diet composition on these patterns are not well characterized.
30316599	5	11	theme	fat	1189:1191	arg1	concentration					1193:1205	fat concentration	1189:1205	fat concentration	1189:1205	Effect of fat concentration and saturation was investigated in the third experiment using a replicated 4 × 4 Latin square design that fed cows 1×/d at 0900 h; treatments included a control diet with no added fat and 2.5% added saturated FA, unsaturated FA, or a mixture of the saturated and unsaturated FA.
30316599	1	12	theme	rumination	198:207	arg1	patterns					170:177	The daily patterns	160:177	The daily patterns of feed intake and rumination	160:207	The daily patterns of feed intake and rumination influence rumen fermentation, rumen pH, and timing of absorbed nutrients in the dairy cow, but the effects of diet composition on these patterns are not well characterized.
30316599	9	13	theme	daily	1944:1948	arg1	patterns					1950:1957	the daily patterns	1940:1957	the daily patterns of rumination or rumen pH	1940:1983	Type of corn silage did not affect the daily patterns of rumination or rumen pH, but pH was reduced throughout the day in brown midrib diets.
30316599	2	14	theme	factorial	716:724	arg1	arrangement					726:736	a 2 × 2 factorial arrangement	708:736	a 2 × 2 factorial arrangement of treatments in cows fed cows 1×/d at 1200 and 1400 h, respectively	708:805	Data from 3 previously published experiments were examined to determine the influence of dietary starch, fiber, and fatty acids (FA) on daily patterns of intake, rumination, and rumen pH. Dietary neutral detergent fiber (NDF) and starch were investigated in 2 experiments, each with duplicated 4 × 4 Latin square designs with a 2 × 2 factorial arrangement of treatments in cows fed cows 1×/d at 1200 and 1400 h, respectively.
30316599	9	15	theme	midrib	2033:2038	arg1	diets					2040:2044	brown midrib diets	2027:2044	brown midrib diets	2027:2044	Type of corn silage did not affect the daily patterns of rumination or rumen pH, but pH was reduced throughout the day in brown midrib diets.
30316599	7	16	theme	starch	1685:1690	arg1	concentration					1692:1704	starch concentration	1685:1704	starch concentration	1685:1704	Rumination displayed a treatment by time interaction for both NDF and starch concentration, with high-fiber, low-starch diets causing greater rumination overnight but not midday.
30316599	5	17	theme	saturated	1456:1464	arg1	FA					1482:1483	the saturated and unsaturated FA	1452:1483	the saturated and unsaturated FA	1452:1483	Effect of fat concentration and saturation was investigated in the third experiment using a replicated 4 × 4 Latin square design that fed cows 1×/d at 0900 h; treatments included a control diet with no added fat and 2.5% added saturated FA, unsaturated FA, or a mixture of the saturated and unsaturated FA.
30316599	2	18	from	arrangement	726:736	arg1	cows					755:758	cows	755:758	cows fed cows 1×/d at 1200 and 1400 h, respectively	755:805	Data from 3 previously published experiments were examined to determine the influence of dietary starch, fiber, and fatty acids (FA) on daily patterns of intake, rumination, and rumen pH. Dietary neutral detergent fiber (NDF) and starch were investigated in 2 experiments, each with duplicated 4 × 4 Latin square designs with a 2 × 2 factorial arrangement of treatments in cows fed cows 1×/d at 1200 and 1400 h, respectively.
30316599	8	19	theme	daily	1890:1894	arg1	pattern					1896:1902	the daily pattern	1886:1902	the daily pattern	1886:1902	High-starch diets decreased total daily rumen pH equally across the day, but did not change the daily pattern.
30316599	5	20	theme	unsaturated	1470:1480	arg1	FA					1482:1483	the saturated and unsaturated FA	1452:1483	the saturated and unsaturated FA	1452:1483	Effect of fat concentration and saturation was investigated in the third experiment using a replicated 4 × 4 Latin square design that fed cows 1×/d at 0900 h; treatments included a control diet with no added fat and 2.5% added saturated FA, unsaturated FA, or a mixture of the saturated and unsaturated FA.
30316599	11	21	theme	fatty	2579:2583	arg1	profile					2590:2596	fatty acid profile	2579:2596	fatty acid profile	2579:2596	Overall, intake, rumination, and rumen pH follow a daily pattern that was minimally modified by dietary fiber and starch type and level or fat level and fatty acid profile.
30316599	4	22	theme	high-starch	1133:1143	arg1	diets					1145:1149	low- and high-starch diets	1124:1149	low- and high-starch diets (21 and 32%, respectively)	1124:1176	To investigate starch source and concentration in the second experiment, ground high-moisture corn or dry ground corn were fed in low- and high-starch diets (21 and 32%, respectively).
30316599	7	23	theme	time	1651:1654	arg1	interaction					1656:1666	time interaction	1651:1666	time interaction	1651:1666	Rumination displayed a treatment by time interaction for both NDF and starch concentration, with high-fiber, low-starch diets causing greater rumination overnight but not midday.
30316599	2	24	theme	fiber	487:491	arg1	influence					458:466	the influence	454:466	the influence of dietary starch, fiber, and fatty acids (FA) on daily patterns of intake, rumination, and rumen pH. Dietary neutral detergent fiber (NDF) and starch were investigated in 2 experiments, each with duplicated 4 × 4 Latin square designs with a 2 × 2 factorial arrangement of treatments in cows fed cows 1×/d at 1200 and 1400 h, respectively	454:805	Data from 3 previously published experiments were examined to determine the influence of dietary starch, fiber, and fatty acids (FA) on daily patterns of intake, rumination, and rumen pH. Dietary neutral detergent fiber (NDF) and starch were investigated in 2 experiments, each with duplicated 4 × 4 Latin square designs with a 2 × 2 factorial arrangement of treatments in cows fed cows 1×/d at 1200 and 1400 h, respectively.
30316599	11	25	theme	dietary	2522:2528	arg1	fiber					2530:2534	dietary fiber	2522:2534	dietary fiber	2522:2534	Overall, intake, rumination, and rumen pH follow a daily pattern that was minimally modified by dietary fiber and starch type and level or fat level and fatty acid profile.
30316599	2	26	theme	Dietary	570:576	arg1	fiber					596:600	pH. Dietary neutral detergent fiber	566:600	pH. Dietary neutral detergent fiber (NDF)	566:606	Data from 3 previously published experiments were examined to determine the influence of dietary starch, fiber, and fatty acids (FA) on daily patterns of intake, rumination, and rumen pH. Dietary neutral detergent fiber (NDF) and starch were investigated in 2 experiments, each with duplicated 4 × 4 Latin square designs with a 2 × 2 factorial arrangement of treatments in cows fed cows 1×/d at 1200 and 1400 h, respectively.
30316599	2	26	theme	Dietary	570:576	arg1	NDF					603:605	NDF	603:605	NDF	603:605	Data from 3 previously published experiments were examined to determine the influence of dietary starch, fiber, and fatty acids (FA) on daily patterns of intake, rumination, and rumen pH. Dietary neutral detergent fiber (NDF) and starch were investigated in 2 experiments, each with duplicated 4 × 4 Latin square designs with a 2 × 2 factorial arrangement of treatments in cows fed cows 1×/d at 1200 and 1400 h, respectively.
30316599	0	27	theme	feed	107:110	arg1	intake					112:117	feed intake	107:117	feed intake	107:117	The effects of source and concentration of dietary fiber, starch, and fatty acids on the daily patterns of feed intake, rumination, and rumen pH in dairy cows.
30316599	2	28	theme	starch	479:484	arg1	influence					458:466	the influence	454:466	the influence of dietary starch, fiber, and fatty acids (FA) on daily patterns of intake, rumination, and rumen pH. Dietary neutral detergent fiber (NDF) and starch were investigated in 2 experiments, each with duplicated 4 × 4 Latin square designs with a 2 × 2 factorial arrangement of treatments in cows fed cows 1×/d at 1200 and 1400 h, respectively	454:805	Data from 3 previously published experiments were examined to determine the influence of dietary starch, fiber, and fatty acids (FA) on daily patterns of intake, rumination, and rumen pH. Dietary neutral detergent fiber (NDF) and starch were investigated in 2 experiments, each with duplicated 4 × 4 Latin square designs with a 2 × 2 factorial arrangement of treatments in cows fed cows 1×/d at 1200 and 1400 h, respectively.
30316599	2	29	from	experiments	415:425	arg1	Data					382:385	Data	382:385	Data from 3 previously published experiments	382:425	Data from 3 previously published experiments were examined to determine the influence of dietary starch, fiber, and fatty acids (FA) on daily patterns of intake, rumination, and rumen pH. Dietary neutral detergent fiber (NDF) and starch were investigated in 2 experiments, each with duplicated 4 × 4 Latin square designs with a 2 × 2 factorial arrangement of treatments in cows fed cows 1×/d at 1200 and 1400 h, respectively.
30316599	2	30	theme	rumen	560:564	arg1	patterns					524:531	daily patterns	518:531	daily patterns of intake, rumination, and rumen pH. Dietary neutral detergent fiber (NDF) and starch were investigated in 2 experiments, each with duplicated 4 × 4 Latin square designs with a 2 × 2 factorial arrangement of treatments in cows fed cows 1×/d at 1200 and 1400 h, respectively	518:805	Data from 3 previously published experiments were examined to determine the influence of dietary starch, fiber, and fatty acids (FA) on daily patterns of intake, rumination, and rumen pH. Dietary neutral detergent fiber (NDF) and starch were investigated in 2 experiments, each with duplicated 4 × 4 Latin square designs with a 2 × 2 factorial arrangement of treatments in cows fed cows 1×/d at 1200 and 1400 h, respectively.
30316599	1	31	theme	composition	324:334	arg1	effects					308:314	the effects	304:314	the effects of diet composition on these patterns	304:352	The daily patterns of feed intake and rumination influence rumen fermentation, rumen pH, and timing of absorbed nutrients in the dairy cow, but the effects of diet composition on these patterns are not well characterized.
30316599	2	32	theme	published	405:413	arg1	experiments					415:425	3 previously published experiments	392:425	3 previously published experiments	392:425	Data from 3 previously published experiments were examined to determine the influence of dietary starch, fiber, and fatty acids (FA) on daily patterns of intake, rumination, and rumen pH. Dietary neutral detergent fiber (NDF) and starch were investigated in 2 experiments, each with duplicated 4 × 4 Latin square designs with a 2 × 2 factorial arrangement of treatments in cows fed cows 1×/d at 1200 and 1400 h, respectively.
30316599	2	33	theme	×	712:712	arg1	arrangement					726:736	a 2 × 2 factorial arrangement	708:736	a 2 × 2 factorial arrangement of treatments in cows fed cows 1×/d at 1200 and 1400 h, respectively	708:805	Data from 3 previously published experiments were examined to determine the influence of dietary starch, fiber, and fatty acids (FA) on daily patterns of intake, rumination, and rumen pH. Dietary neutral detergent fiber (NDF) and starch were investigated in 2 experiments, each with duplicated 4 × 4 Latin square designs with a 2 × 2 factorial arrangement of treatments in cows fed cows 1×/d at 1200 and 1400 h, respectively.
30316599	2	34	dep	patterns	524:531	arg1	investigated					624:635	investigated	624:635	were investigated in 2 experiments, each with duplicated 4 × 4 Latin square designs with a 2 × 2 factorial arrangement of treatments in cows fed cows 1×/d at 1200 and 1400 h, respectively	619:805	Data from 3 previously published experiments were examined to determine the influence of dietary starch, fiber, and fatty acids (FA) on daily patterns of intake, rumination, and rumen pH. Dietary neutral detergent fiber (NDF) and starch were investigated in 2 experiments, each with duplicated 4 × 4 Latin square designs with a 2 × 2 factorial arrangement of treatments in cows fed cows 1×/d at 1200 and 1400 h, respectively.
30316599	10	35	located	observed	2139:2146	arg1	experiment					2060:2069	the third experiment	2050:2069	the third experiment	2050:2069	In the third experiment, no interactions between fatty acid supplement and time of day were observed for intake, rumination, or rumen pH. Within all experiments, rumination fit or tended to fit a 24-h rhythm regardless of diet, with the amplitude of the rumination being reduced in low-starch diets and diets containing saturated FA or a mixture of saturated and unsaturated FA.
30316599	10	35	located	observed	2139:2146	arg2	interactions					2075:2086	no interactions	2072:2086	no interactions between fatty acid supplement and time of day	2072:2132	In the third experiment, no interactions between fatty acid supplement and time of day were observed for intake, rumination, or rumen pH. Within all experiments, rumination fit or tended to fit a 24-h rhythm regardless of diet, with the amplitude of the rumination being reduced in low-starch diets and diets containing saturated FA or a mixture of saturated and unsaturated FA.
30316599	2	36	theme	detergent	586:594	arg1	fiber					596:600	pH. Dietary neutral detergent fiber	566:600	pH. Dietary neutral detergent fiber (NDF)	566:606	Data from 3 previously published experiments were examined to determine the influence of dietary starch, fiber, and fatty acids (FA) on daily patterns of intake, rumination, and rumen pH. Dietary neutral detergent fiber (NDF) and starch were investigated in 2 experiments, each with duplicated 4 × 4 Latin square designs with a 2 × 2 factorial arrangement of treatments in cows fed cows 1×/d at 1200 and 1400 h, respectively.
30316599	2	36	theme	detergent	586:594	arg1	NDF					603:605	NDF	603:605	NDF	603:605	Data from 3 previously published experiments were examined to determine the influence of dietary starch, fiber, and fatty acids (FA) on daily patterns of intake, rumination, and rumen pH. Dietary neutral detergent fiber (NDF) and starch were investigated in 2 experiments, each with duplicated 4 × 4 Latin square designs with a 2 × 2 factorial arrangement of treatments in cows fed cows 1×/d at 1200 and 1400 h, respectively.
30316599	9	37	theme	corn	1913:1916	arg1	silage					1918:1923	corn silage	1913:1923	corn silage	1913:1923	Type of corn silage did not affect the daily patterns of rumination or rumen pH, but pH was reduced throughout the day in brown midrib diets.
30316599	0	38	theme	starch	58:63	arg1	concentration					26:38	concentration	26:38	concentration	26:38	The effects of source and concentration of dietary fiber, starch, and fatty acids on the daily patterns of feed intake, rumination, and rumen pH in dairy cows.
30316599	0	38	theme	starch	58:63	arg1	source					15:20	source	15:20	source	15:20	The effects of source and concentration of dietary fiber, starch, and fatty acids on the daily patterns of feed intake, rumination, and rumen pH in dairy cows.
30316599	1	39	theme	absorbed	263:270	arg1	nutrients					272:280	absorbed nutrients	263:280	absorbed nutrients	263:280	The daily patterns of feed intake and rumination influence rumen fermentation, rumen pH, and timing of absorbed nutrients in the dairy cow, but the effects of diet composition on these patterns are not well characterized.
30316599	3	40	theme	brown	880:884	arg1	midrib					886:891	brown midrib	880:891	brown midrib	880:891	To investigate fiber content and digestibility in the first experiment, brown midrib or isogenic conventional corn silage were fed in low- and high-NDF diets (29 and 38%, respectively).
30316599	0	41	theme	rumen	136:140	arg1	pH					142:143	rumen pH	136:143	rumen pH	136:143	The effects of source and concentration of dietary fiber, starch, and fatty acids on the daily patterns of feed intake, rumination, and rumen pH in dairy cows.
30316599	5	42	theme	third	1246:1250	arg1	experiment					1252:1261	the third experiment	1242:1261	the third experiment using a replicated 4 × 4 Latin square design that fed cows 1×/d at 0900 h	1242:1335	Effect of fat concentration and saturation was investigated in the third experiment using a replicated 4 × 4 Latin square design that fed cows 1×/d at 0900 h; treatments included a control diet with no added fat and 2.5% added saturated FA, unsaturated FA, or a mixture of the saturated and unsaturated FA.
30316599	4	43	theme	starch	1009:1014	arg1	source					1016:1021	starch source	1009:1021	starch source	1009:1021	To investigate starch source and concentration in the second experiment, ground high-moisture corn or dry ground corn were fed in low- and high-starch diets (21 and 32%, respectively).
30316599	10	44	theme	rumination	2209:2218	arg1	fit					2220:2222	rumination fit	2209:2222	rumination fit	2209:2222	In the third experiment, no interactions between fatty acid supplement and time of day were observed for intake, rumination, or rumen pH. Within all experiments, rumination fit or tended to fit a 24-h rhythm regardless of diet, with the amplitude of the rumination being reduced in low-starch diets and diets containing saturated FA or a mixture of saturated and unsaturated FA.
30316599	1	45	from	effects	308:314	arg1	patterns					345:352	these patterns	339:352	these patterns	339:352	The daily patterns of feed intake and rumination influence rumen fermentation, rumen pH, and timing of absorbed nutrients in the dairy cow, but the effects of diet composition on these patterns are not well characterized.
30316599	10	46	theme	saturated	2367:2375	arg1	FA					2377:2378	saturated FA	2367:2378	saturated FA	2367:2378	In the third experiment, no interactions between fatty acid supplement and time of day were observed for intake, rumination, or rumen pH. Within all experiments, rumination fit or tended to fit a 24-h rhythm regardless of diet, with the amplitude of the rumination being reduced in low-starch diets and diets containing saturated FA or a mixture of saturated and unsaturated FA.
30316599	2	47	theme	daily	518:522	arg1	patterns					524:531	daily patterns	518:531	daily patterns of intake, rumination, and rumen pH. Dietary neutral detergent fiber (NDF) and starch were investigated in 2 experiments, each with duplicated 4 × 4 Latin square designs with a 2 × 2 factorial arrangement of treatments in cows fed cows 1×/d at 1200 and 1400 h, respectively	518:805	Data from 3 previously published experiments were examined to determine the influence of dietary starch, fiber, and fatty acids (FA) on daily patterns of intake, rumination, and rumen pH. Dietary neutral detergent fiber (NDF) and starch were investigated in 2 experiments, each with duplicated 4 × 4 Latin square designs with a 2 × 2 factorial arrangement of treatments in cows fed cows 1×/d at 1200 and 1400 h, respectively.
30316599	0	48	theme	acids	76:80	arg1	concentration					26:38	concentration	26:38	concentration	26:38	The effects of source and concentration of dietary fiber, starch, and fatty acids on the daily patterns of feed intake, rumination, and rumen pH in dairy cows.
30316599	0	48	theme	acids	76:80	arg1	source					15:20	source	15:20	source	15:20	The effects of source and concentration of dietary fiber, starch, and fatty acids on the daily patterns of feed intake, rumination, and rumen pH in dairy cows.
30316599	1	49	theme	dairy	289:293	arg1	cow					295:297	the dairy cow	285:297	the dairy cow	285:297	The daily patterns of feed intake and rumination influence rumen fermentation, rumen pH, and timing of absorbed nutrients in the dairy cow, but the effects of diet composition on these patterns are not well characterized.
30316599	3	50	theme	conventional	905:916	arg1	silage					923:928	isogenic conventional corn silage	896:928	isogenic conventional corn silage	896:928	To investigate fiber content and digestibility in the first experiment, brown midrib or isogenic conventional corn silage were fed in low- and high-NDF diets (29 and 38%, respectively).
30316599	5	51	theme	saturated	1406:1414	arg1	FA					1416:1417	saturated FA	1406:1417	saturated FA	1406:1417	Effect of fat concentration and saturation was investigated in the third experiment using a replicated 4 × 4 Latin square design that fed cows 1×/d at 0900 h; treatments included a control diet with no added fat and 2.5% added saturated FA, unsaturated FA, or a mixture of the saturated and unsaturated FA.
30316599	11	52	theme	rumen	2459:2463	arg1	pH					2465:2466	rumen pH	2459:2466	rumen pH	2459:2466	Overall, intake, rumination, and rumen pH follow a daily pattern that was minimally modified by dietary fiber and starch type and level or fat level and fatty acid profile.
30316599	1	53	from	fermentation	225:236	arg1	cow					295:297	the dairy cow	285:297	the dairy cow	285:297	The daily patterns of feed intake and rumination influence rumen fermentation, rumen pH, and timing of absorbed nutrients in the dairy cow, but the effects of diet composition on these patterns are not well characterized.
30316599	2	54	theme	treatments	741:750	arg1	arrangement					726:736	a 2 × 2 factorial arrangement	708:736	a 2 × 2 factorial arrangement of treatments in cows fed cows 1×/d at 1200 and 1400 h, respectively	708:805	Data from 3 previously published experiments were examined to determine the influence of dietary starch, fiber, and fatty acids (FA) on daily patterns of intake, rumination, and rumen pH. Dietary neutral detergent fiber (NDF) and starch were investigated in 2 experiments, each with duplicated 4 × 4 Latin square designs with a 2 × 2 factorial arrangement of treatments in cows fed cows 1×/d at 1200 and 1400 h, respectively.
30316599	5	55	theme	×	1284:1284	arg1	design					1301:1306	a replicated 4 × 4 Latin square design	1269:1306	a replicated 4 × 4 Latin square design that fed cows 1×/d at 0900 h	1269:1335	Effect of fat concentration and saturation was investigated in the third experiment using a replicated 4 × 4 Latin square design that fed cows 1×/d at 0900 h; treatments included a control diet with no added fat and 2.5% added saturated FA, unsaturated FA, or a mixture of the saturated and unsaturated FA.
30316599	5	56	theme	added	1381:1385	arg1	fat					1387:1389	fat	1387:1389	fat	1387:1389	Effect of fat concentration and saturation was investigated in the third experiment using a replicated 4 × 4 Latin square design that fed cows 1×/d at 0900 h; treatments included a control diet with no added fat and 2.5% added saturated FA, unsaturated FA, or a mixture of the saturated and unsaturated FA.
30316599	10	57	theme	acid	2102:2105	arg1	supplement					2107:2116	fatty acid supplement	2096:2116	fatty acid supplement	2096:2116	In the third experiment, no interactions between fatty acid supplement and time of day were observed for intake, rumination, or rumen pH. Within all experiments, rumination fit or tended to fit a 24-h rhythm regardless of diet, with the amplitude of the rumination being reduced in low-starch diets and diets containing saturated FA or a mixture of saturated and unsaturated FA.
30316599	1	58	theme	rumen	239:243	arg1	pH					245:246	rumen pH	239:246	rumen pH	239:246	The daily patterns of feed intake and rumination influence rumen fermentation, rumen pH, and timing of absorbed nutrients in the dairy cow, but the effects of diet composition on these patterns are not well characterized.
30316599	8	59	theme	High-starch	1794:1804	arg1	diets					1806:1810	High-starch diets	1794:1810	High-starch diets	1794:1810	High-starch diets decreased total daily rumen pH equally across the day, but did not change the daily pattern.
30316599	0	60	from	cows	154:157	arg1	patterns					95:102	the daily patterns	85:102	the daily patterns of feed intake, rumination, and rumen pH in dairy cows	85:157	The effects of source and concentration of dietary fiber, starch, and fatty acids on the daily patterns of feed intake, rumination, and rumen pH in dairy cows.
30316599	0	61	theme	fiber	51:55	arg1	concentration					26:38	concentration	26:38	concentration	26:38	The effects of source and concentration of dietary fiber, starch, and fatty acids on the daily patterns of feed intake, rumination, and rumen pH in dairy cows.
30316599	0	61	theme	fiber	51:55	arg1	source					15:20	source	15:20	source	15:20	The effects of source and concentration of dietary fiber, starch, and fatty acids on the daily patterns of feed intake, rumination, and rumen pH in dairy cows.
30316599	9	62	theme	pH	1982:1983	arg1	patterns					1950:1957	the daily patterns	1940:1957	the daily patterns of rumination or rumen pH	1940:1983	Type of corn silage did not affect the daily patterns of rumination or rumen pH, but pH was reduced throughout the day in brown midrib diets.
30316599	3	63	theme	high-NDF	951:958	arg1	diets					960:964	low- and high-NDF diets	942:964	low- and high-NDF diets (29 and 38%, respectively)	942:991	To investigate fiber content and digestibility in the first experiment, brown midrib or isogenic conventional corn silage were fed in low- and high-NDF diets (29 and 38%, respectively).
30316599	3	64	from	digestibility	841:853	arg1	experiment					868:877	the first experiment	858:877	the first experiment	858:877	To investigate fiber content and digestibility in the first experiment, brown midrib or isogenic conventional corn silage were fed in low- and high-NDF diets (29 and 38%, respectively).
30316599	2	65	theme	duplicated	665:674	arg1	designs					695:701	duplicated 4 × 4 Latin square designs	665:701	duplicated 4 × 4 Latin square designs	665:701	Data from 3 previously published experiments were examined to determine the influence of dietary starch, fiber, and fatty acids (FA) on daily patterns of intake, rumination, and rumen pH. Dietary neutral detergent fiber (NDF) and starch were investigated in 2 experiments, each with duplicated 4 × 4 Latin square designs with a 2 × 2 factorial arrangement of treatments in cows fed cows 1×/d at 1200 and 1400 h, respectively.
30316599	10	66	theme	saturated	2396:2404	arg1	FA					2422:2423	saturated and unsaturated FA	2396:2423	saturated and unsaturated FA	2396:2423	In the third experiment, no interactions between fatty acid supplement and time of day were observed for intake, rumination, or rumen pH. Within all experiments, rumination fit or tended to fit a 24-h rhythm regardless of diet, with the amplitude of the rumination being reduced in low-starch diets and diets containing saturated FA or a mixture of saturated and unsaturated FA.
30316599	5	67	theme	replicated	1271:1280	arg1	design					1301:1306	a replicated 4 × 4 Latin square design	1269:1306	a replicated 4 × 4 Latin square design that fed cows 1×/d at 0900 h	1269:1335	Effect of fat concentration and saturation was investigated in the third experiment using a replicated 4 × 4 Latin square design that fed cows 1×/d at 0900 h; treatments included a control diet with no added fat and 2.5% added saturated FA, unsaturated FA, or a mixture of the saturated and unsaturated FA.
30316599	3	68	theme	low-	942:945	arg1	diets					960:964	low- and high-NDF diets	942:964	low- and high-NDF diets (29 and 38%, respectively)	942:991	To investigate fiber content and digestibility in the first experiment, brown midrib or isogenic conventional corn silage were fed in low- and high-NDF diets (29 and 38%, respectively).
30316599	8	69	theme	daily	1828:1832	arg1	pH					1840:1841	total daily rumen pH	1822:1841	total daily rumen pH	1822:1841	High-starch diets decreased total daily rumen pH equally across the day, but did not change the daily pattern.
30316599	0	70	from	patterns	95:102	arg1	cows					154:157	dairy cows	148:157	dairy cows	148:157	The effects of source and concentration of dietary fiber, starch, and fatty acids on the daily patterns of feed intake, rumination, and rumen pH in dairy cows.
30316599	4	71	theme	second	1048:1053	arg1	experiment					1055:1064	the second experiment	1044:1064	the second experiment	1044:1064	To investigate starch source and concentration in the second experiment, ground high-moisture corn or dry ground corn were fed in low- and high-starch diets (21 and 32%, respectively).
30316599	0	72	theme	dietary	43:49	arg1	fiber					51:55	dietary fiber	43:55	dietary fiber	43:55	The effects of source and concentration of dietary fiber, starch, and fatty acids on the daily patterns of feed intake, rumination, and rumen pH in dairy cows.
30316599	2	73	theme	Latin	682:686	arg1	square					688:693	4 × 4 Latin square	676:693	duplicated 4 × 4 Latin square designs	665:701	Data from 3 previously published experiments were examined to determine the influence of dietary starch, fiber, and fatty acids (FA) on daily patterns of intake, rumination, and rumen pH. Dietary neutral detergent fiber (NDF) and starch were investigated in 2 experiments, each with duplicated 4 × 4 Latin square designs with a 2 × 2 factorial arrangement of treatments in cows fed cows 1×/d at 1200 and 1400 h, respectively.
30316599	2	74	from	influence	458:466	arg1	patterns					524:531	daily patterns	518:531	daily patterns of intake, rumination, and rumen pH. Dietary neutral detergent fiber (NDF) and starch were investigated in 2 experiments, each with duplicated 4 × 4 Latin square designs with a 2 × 2 factorial arrangement of treatments in cows fed cows 1×/d at 1200 and 1400 h, respectively	518:805	Data from 3 previously published experiments were examined to determine the influence of dietary starch, fiber, and fatty acids (FA) on daily patterns of intake, rumination, and rumen pH. Dietary neutral detergent fiber (NDF) and starch were investigated in 2 experiments, each with duplicated 4 × 4 Latin square designs with a 2 × 2 factorial arrangement of treatments in cows fed cows 1×/d at 1200 and 1400 h, respectively.
30316599	1	75	theme	feed	182:185	arg1	intake					187:192	feed intake	182:192	feed intake	182:192	The daily patterns of feed intake and rumination influence rumen fermentation, rumen pH, and timing of absorbed nutrients in the dairy cow, but the effects of diet composition on these patterns are not well characterized.
30316599	2	76	theme	fatty	498:502	arg1	FA					511:512	FA	511:512	FA	511:512	Data from 3 previously published experiments were examined to determine the influence of dietary starch, fiber, and fatty acids (FA) on daily patterns of intake, rumination, and rumen pH. Dietary neutral detergent fiber (NDF) and starch were investigated in 2 experiments, each with duplicated 4 × 4 Latin square designs with a 2 × 2 factorial arrangement of treatments in cows fed cows 1×/d at 1200 and 1400 h, respectively.
30316599	2	76	theme	fatty	498:502	arg1	acids					504:508	fatty acids	498:508	fatty acids (FA)	498:513	Data from 3 previously published experiments were examined to determine the influence of dietary starch, fiber, and fatty acids (FA) on daily patterns of intake, rumination, and rumen pH. Dietary neutral detergent fiber (NDF) and starch were investigated in 2 experiments, each with duplicated 4 × 4 Latin square designs with a 2 × 2 factorial arrangement of treatments in cows fed cows 1×/d at 1200 and 1400 h, respectively.
30316599	3	77	theme	fiber	823:827	arg1	content					829:835	fiber content	823:835	fiber content	823:835	To investigate fiber content and digestibility in the first experiment, brown midrib or isogenic conventional corn silage were fed in low- and high-NDF diets (29 and 38%, respectively).
30316599	3	78	from	content	829:835	arg1	experiment					868:877	the first experiment	858:877	the first experiment	858:877	To investigate fiber content and digestibility in the first experiment, brown midrib or isogenic conventional corn silage were fed in low- and high-NDF diets (29 and 38%, respectively).
30316599	2	79	theme	×	678:678	arg1	square					688:693	4 × 4 Latin square	676:693	duplicated 4 × 4 Latin square designs	665:701	Data from 3 previously published experiments were examined to determine the influence of dietary starch, fiber, and fatty acids (FA) on daily patterns of intake, rumination, and rumen pH. Dietary neutral detergent fiber (NDF) and starch were investigated in 2 experiments, each with duplicated 4 × 4 Latin square designs with a 2 × 2 factorial arrangement of treatments in cows fed cows 1×/d at 1200 and 1400 h, respectively.
30316599	0	80	theme	pH	142:143	arg1	patterns					95:102	the daily patterns	85:102	the daily patterns of feed intake, rumination, and rumen pH in dairy cows	85:157	The effects of source and concentration of dietary fiber, starch, and fatty acids on the daily patterns of feed intake, rumination, and rumen pH in dairy cows.
30316599	5	81	theme	FA	1482:1483	arg1	FA					1432:1433	unsaturated FA	1420:1433	unsaturated FA	1420:1433	Effect of fat concentration and saturation was investigated in the third experiment using a replicated 4 × 4 Latin square design that fed cows 1×/d at 0900 h; treatments included a control diet with no added fat and 2.5% added saturated FA, unsaturated FA, or a mixture of the saturated and unsaturated FA.
30316599	5	81	theme	FA	1482:1483	arg1	mixture					1441:1447	a mixture	1439:1447	a mixture of the saturated and unsaturated FA	1439:1483	Effect of fat concentration and saturation was investigated in the third experiment using a replicated 4 × 4 Latin square design that fed cows 1×/d at 0900 h; treatments included a control diet with no added fat and 2.5% added saturated FA, unsaturated FA, or a mixture of the saturated and unsaturated FA.
30316599	5	81	theme	FA	1482:1483	arg1	FA					1416:1417	saturated FA	1406:1417	saturated FA	1406:1417	Effect of fat concentration and saturation was investigated in the third experiment using a replicated 4 × 4 Latin square design that fed cows 1×/d at 0900 h; treatments included a control diet with no added fat and 2.5% added saturated FA, unsaturated FA, or a mixture of the saturated and unsaturated FA.
30316599	11	82	mod	modified	2510:2517	arg1	pattern					2483:2489	a daily pattern	2475:2489	a daily pattern that was minimally modified by dietary fiber and starch type and level or fat level and fatty acid profile	2475:2596	Overall, intake, rumination, and rumen pH follow a daily pattern that was minimally modified by dietary fiber and starch type and level or fat level and fatty acid profile.
30316599	11	82	mod	modified	2510:2517	arg3	level					2556:2560	level	2556:2560	level	2556:2560	Overall, intake, rumination, and rumen pH follow a daily pattern that was minimally modified by dietary fiber and starch type and level or fat level and fatty acid profile.
30316599	11	82	mod	modified	2510:2517	arg3	fiber					2530:2534	dietary fiber	2522:2534	dietary fiber	2522:2534	Overall, intake, rumination, and rumen pH follow a daily pattern that was minimally modified by dietary fiber and starch type and level or fat level and fatty acid profile.
30316599	11	82	mod	modified	2510:2517	arg3	type					2547:2550	starch type	2540:2550	starch type	2540:2550	Overall, intake, rumination, and rumen pH follow a daily pattern that was minimally modified by dietary fiber and starch type and level or fat level and fatty acid profile.
30316599	6	83	theme	first	1493:1497	arg1	experiments					1501:1511	the first 2 experiments	1489:1511	the first 2 experiments	1489:1511	In the first 2 experiments, intake followed a similar daily pattern regardless of starch and NDF concentration or digestibility.
30316599	0	84	theme	dairy	148:152	arg1	cows					154:157	dairy cows	148:157	dairy cows	148:157	The effects of source and concentration of dietary fiber, starch, and fatty acids on the daily patterns of feed intake, rumination, and rumen pH in dairy cows.
30316599	5	85	theme	concentration	1193:1205	arg1	Effect					1179:1184	Effect	1179:1184	Effect of fat concentration and saturation	1179:1220	Effect of fat concentration and saturation was investigated in the third experiment using a replicated 4 × 4 Latin square design that fed cows 1×/d at 0900 h; treatments included a control diet with no added fat and 2.5% added saturated FA, unsaturated FA, or a mixture of the saturated and unsaturated FA.
30316599	9	86	theme	brown	2027:2031	arg1	diets					2040:2044	brown midrib diets	2027:2044	brown midrib diets	2027:2044	Type of corn silage did not affect the daily patterns of rumination or rumen pH, but pH was reduced throughout the day in brown midrib diets.
30316599	11	87	theme	fat	2565:2567	arg1	level					2569:2573	fat level	2565:2573	fat level	2565:2573	Overall, intake, rumination, and rumen pH follow a daily pattern that was minimally modified by dietary fiber and starch type and level or fat level and fatty acid profile.
30316599	6	88	theme	NDF	1579:1581	arg1	concentration					1583:1595	starch and NDF concentration	1568:1595	starch and NDF concentration	1568:1595	In the first 2 experiments, intake followed a similar daily pattern regardless of starch and NDF concentration or digestibility.
30316599	5	89	theme	control	1360:1366	arg1	diet					1368:1371	a control diet	1358:1371	a control diet	1358:1371	Effect of fat concentration and saturation was investigated in the third experiment using a replicated 4 × 4 Latin square design that fed cows 1×/d at 0900 h; treatments included a control diet with no added fat and 2.5% added saturated FA, unsaturated FA, or a mixture of the saturated and unsaturated FA.
30316599	4	90	theme	ground	1100:1105	arg1	corn					1107:1110	dry ground corn	1096:1110	dry ground corn	1096:1110	To investigate starch source and concentration in the second experiment, ground high-moisture corn or dry ground corn were fed in low- and high-starch diets (21 and 32%, respectively).
30316599	10	91	theme	low-starch	2329:2338	arg1	diets					2340:2344	low-starch diets	2329:2344	low-starch diets	2329:2344	In the third experiment, no interactions between fatty acid supplement and time of day were observed for intake, rumination, or rumen pH. Within all experiments, rumination fit or tended to fit a 24-h rhythm regardless of diet, with the amplitude of the rumination being reduced in low-starch diets and diets containing saturated FA or a mixture of saturated and unsaturated FA.
30316599	6	92	theme	starch	1568:1573	arg1	concentration					1583:1595	starch and NDF concentration	1568:1595	starch and NDF concentration	1568:1595	In the first 2 experiments, intake followed a similar daily pattern regardless of starch and NDF concentration or digestibility.
30316599	0	93	theme	daily	89:93	arg1	patterns					95:102	the daily patterns	85:102	the daily patterns of feed intake, rumination, and rumen pH in dairy cows	85:157	The effects of source and concentration of dietary fiber, starch, and fatty acids on the daily patterns of feed intake, rumination, and rumen pH in dairy cows.
30316599	7	94	dep	overnight	1768:1776	arg1	greater					1749:1755	greater	1749:1755	greater	1749:1755	Rumination displayed a treatment by time interaction for both NDF and starch concentration, with high-fiber, low-starch diets causing greater rumination overnight but not midday.
30316599	11	95	theme	acid	2585:2588	arg1	profile					2590:2596	fatty acid profile	2579:2596	fatty acid profile	2579:2596	Overall, intake, rumination, and rumen pH follow a daily pattern that was minimally modified by dietary fiber and starch type and level or fat level and fatty acid profile.
30316599	2	96	theme	neutral	578:584	arg1	fiber					596:600	pH. Dietary neutral detergent fiber	566:600	pH. Dietary neutral detergent fiber (NDF)	566:606	Data from 3 previously published experiments were examined to determine the influence of dietary starch, fiber, and fatty acids (FA) on daily patterns of intake, rumination, and rumen pH. Dietary neutral detergent fiber (NDF) and starch were investigated in 2 experiments, each with duplicated 4 × 4 Latin square designs with a 2 × 2 factorial arrangement of treatments in cows fed cows 1×/d at 1200 and 1400 h, respectively.
30316599	2	96	theme	neutral	578:584	arg1	NDF					603:605	NDF	603:605	NDF	603:605	Data from 3 previously published experiments were examined to determine the influence of dietary starch, fiber, and fatty acids (FA) on daily patterns of intake, rumination, and rumen pH. Dietary neutral detergent fiber (NDF) and starch were investigated in 2 experiments, each with duplicated 4 × 4 Latin square designs with a 2 × 2 factorial arrangement of treatments in cows fed cows 1×/d at 1200 and 1400 h, respectively.
30316599	6	97	theme	daily	1540:1544	arg1	pattern					1546:1552	a similar daily pattern	1530:1552	a similar daily pattern regardless of starch and NDF concentration or digestibility	1530:1612	In the first 2 experiments, intake followed a similar daily pattern regardless of starch and NDF concentration or digestibility.
30316599	0	98	theme	intake	112:117	arg1	patterns					95:102	the daily patterns	85:102	the daily patterns of feed intake, rumination, and rumen pH in dairy cows	85:157	The effects of source and concentration of dietary fiber, starch, and fatty acids on the daily patterns of feed intake, rumination, and rumen pH in dairy cows.
30316599	3	99	dep	diets	960:964	arg1	%					976:976	29 and 38%	967:976	29 and 38%	967:976	To investigate fiber content and digestibility in the first experiment, brown midrib or isogenic conventional corn silage were fed in low- and high-NDF diets (29 and 38%, respectively).
30316599	2	100	theme	pH.	566:568	arg1	fiber					596:600	pH. Dietary neutral detergent fiber	566:600	pH. Dietary neutral detergent fiber (NDF)	566:606	Data from 3 previously published experiments were examined to determine the influence of dietary starch, fiber, and fatty acids (FA) on daily patterns of intake, rumination, and rumen pH. Dietary neutral detergent fiber (NDF) and starch were investigated in 2 experiments, each with duplicated 4 × 4 Latin square designs with a 2 × 2 factorial arrangement of treatments in cows fed cows 1×/d at 1200 and 1400 h, respectively.
30316599	2	100	theme	pH.	566:568	arg1	NDF					603:605	NDF	603:605	NDF	603:605	Data from 3 previously published experiments were examined to determine the influence of dietary starch, fiber, and fatty acids (FA) on daily patterns of intake, rumination, and rumen pH. Dietary neutral detergent fiber (NDF) and starch were investigated in 2 experiments, each with duplicated 4 × 4 Latin square designs with a 2 × 2 factorial arrangement of treatments in cows fed cows 1×/d at 1200 and 1400 h, respectively.
30316599	10	101	theme	unsaturated	2410:2420	arg1	FA					2422:2423	saturated and unsaturated FA	2396:2423	saturated and unsaturated FA	2396:2423	In the third experiment, no interactions between fatty acid supplement and time of day were observed for intake, rumination, or rumen pH. Within all experiments, rumination fit or tended to fit a 24-h rhythm regardless of diet, with the amplitude of the rumination being reduced in low-starch diets and diets containing saturated FA or a mixture of saturated and unsaturated FA.
30316599	0	102	theme	rumination	120:129	arg1	patterns					95:102	the daily patterns	85:102	the daily patterns of feed intake, rumination, and rumen pH in dairy cows	85:157	The effects of source and concentration of dietary fiber, starch, and fatty acids on the daily patterns of feed intake, rumination, and rumen pH in dairy cows.
30316599	1	103	theme	diet	319:322	arg1	composition					324:334	diet composition	319:334	diet composition	319:334	The daily patterns of feed intake and rumination influence rumen fermentation, rumen pH, and timing of absorbed nutrients in the dairy cow, but the effects of diet composition on these patterns are not well characterized.
30316599	2	104	theme	dietary	471:477	arg1	starch					479:484	dietary starch	471:484	dietary starch	471:484	Data from 3 previously published experiments were examined to determine the influence of dietary starch, fiber, and fatty acids (FA) on daily patterns of intake, rumination, and rumen pH. Dietary neutral detergent fiber (NDF) and starch were investigated in 2 experiments, each with duplicated 4 × 4 Latin square designs with a 2 × 2 factorial arrangement of treatments in cows fed cows 1×/d at 1200 and 1400 h, respectively.
30316599	10	105	theme	rumination	2301:2310	arg1	amplitude					2284:2292	the amplitude	2280:2292	the amplitude of the rumination	2280:2310	In the third experiment, no interactions between fatty acid supplement and time of day were observed for intake, rumination, or rumen pH. Within all experiments, rumination fit or tended to fit a 24-h rhythm regardless of diet, with the amplitude of the rumination being reduced in low-starch diets and diets containing saturated FA or a mixture of saturated and unsaturated FA.
30316599	0	106	from	effects	4:10	arg1	patterns					95:102	the daily patterns	85:102	the daily patterns of feed intake, rumination, and rumen pH in dairy cows	85:157	The effects of source and concentration of dietary fiber, starch, and fatty acids on the daily patterns of feed intake, rumination, and rumen pH in dairy cows.
30316599	2	107	theme	intake	536:541	arg1	patterns					524:531	daily patterns	518:531	daily patterns of intake, rumination, and rumen pH. Dietary neutral detergent fiber (NDF) and starch were investigated in 2 experiments, each with duplicated 4 × 4 Latin square designs with a 2 × 2 factorial arrangement of treatments in cows fed cows 1×/d at 1200 and 1400 h, respectively	518:805	Data from 3 previously published experiments were examined to determine the influence of dietary starch, fiber, and fatty acids (FA) on daily patterns of intake, rumination, and rumen pH. Dietary neutral detergent fiber (NDF) and starch were investigated in 2 experiments, each with duplicated 4 × 4 Latin square designs with a 2 × 2 factorial arrangement of treatments in cows fed cows 1×/d at 1200 and 1400 h, respectively.
30316599	9	108	theme	silage	1918:1923	arg1	Type					1905:1908	Type	1905:1908	Type of corn silage	1905:1923	Type of corn silage did not affect the daily patterns of rumination or rumen pH, but pH was reduced throughout the day in brown midrib diets.
30316599	0	109	theme	fatty	70:74	arg1	acids					76:80	fatty acids	70:80	fatty acids	70:80	The effects of source and concentration of dietary fiber, starch, and fatty acids on the daily patterns of feed intake, rumination, and rumen pH in dairy cows.
30316599	1	110	theme	nutrients	272:280	arg1	fermentation					225:236	rumen fermentation	219:236	rumen fermentation	219:236	The daily patterns of feed intake and rumination influence rumen fermentation, rumen pH, and timing of absorbed nutrients in the dairy cow, but the effects of diet composition on these patterns are not well characterized.
30316599	1	110	theme	nutrients	272:280	arg1	pH					245:246	rumen pH	239:246	rumen pH	239:246	The daily patterns of feed intake and rumination influence rumen fermentation, rumen pH, and timing of absorbed nutrients in the dairy cow, but the effects of diet composition on these patterns are not well characterized.
30316599	1	110	theme	nutrients	272:280	arg1	timing					253:258	timing	253:258	timing	253:258	The daily patterns of feed intake and rumination influence rumen fermentation, rumen pH, and timing of absorbed nutrients in the dairy cow, but the effects of diet composition on these patterns are not well characterized.
30316599	3	111	theme	first	862:866	arg1	experiment					868:877	the first experiment	858:877	the first experiment	858:877	To investigate fiber content and digestibility in the first experiment, brown midrib or isogenic conventional corn silage were fed in low- and high-NDF diets (29 and 38%, respectively).
30316599	3	112	theme	isogenic	896:903	arg1	silage					923:928	isogenic conventional corn silage	896:928	isogenic conventional corn silage	896:928	To investigate fiber content and digestibility in the first experiment, brown midrib or isogenic conventional corn silage were fed in low- and high-NDF diets (29 and 38%, respectively).
30316599	5	113	theme	saturation	1211:1220	arg1	Effect					1179:1184	Effect	1179:1184	Effect of fat concentration and saturation	1179:1220	Effect of fat concentration and saturation was investigated in the third experiment using a replicated 4 × 4 Latin square design that fed cows 1×/d at 0900 h; treatments included a control diet with no added fat and 2.5% added saturated FA, unsaturated FA, or a mixture of the saturated and unsaturated FA.
30316599	0	114	from	pH	142:143	arg1	cows					154:157	dairy cows	148:157	dairy cows	148:157	The effects of source and concentration of dietary fiber, starch, and fatty acids on the daily patterns of feed intake, rumination, and rumen pH in dairy cows.
30316599	2	115	theme	rumination	544:553	arg1	patterns					524:531	daily patterns	518:531	daily patterns of intake, rumination, and rumen pH. Dietary neutral detergent fiber (NDF) and starch were investigated in 2 experiments, each with duplicated 4 × 4 Latin square designs with a 2 × 2 factorial arrangement of treatments in cows fed cows 1×/d at 1200 and 1400 h, respectively	518:805	Data from 3 previously published experiments were examined to determine the influence of dietary starch, fiber, and fatty acids (FA) on daily patterns of intake, rumination, and rumen pH. Dietary neutral detergent fiber (NDF) and starch were investigated in 2 experiments, each with duplicated 4 × 4 Latin square designs with a 2 × 2 factorial arrangement of treatments in cows fed cows 1×/d at 1200 and 1400 h, respectively.
30316599	11	116	theme	starch	2540:2545	arg1	type					2547:2550	starch type	2540:2550	starch type	2540:2550	Overall, intake, rumination, and rumen pH follow a daily pattern that was minimally modified by dietary fiber and starch type and level or fat level and fatty acid profile.
30316599	0	117	theme	source	15:20	arg1	effects					4:10	The effects	0:10	The effects of source and concentration of dietary fiber, starch, and fatty acids on the daily patterns of feed intake, rumination, and rumen pH in dairy cows.	0:158	The effects of source and concentration of dietary fiber, starch, and fatty acids on the daily patterns of feed intake, rumination, and rumen pH in dairy cows.
30316599	1	118	theme	rumen	219:223	arg1	fermentation					225:236	rumen fermentation	219:236	rumen fermentation	219:236	The daily patterns of feed intake and rumination influence rumen fermentation, rumen pH, and timing of absorbed nutrients in the dairy cow, but the effects of diet composition on these patterns are not well characterized.
30316599	10	119	contain	containing	2356:2365	arg1	diets					2340:2344	low-starch diets	2329:2344	low-starch diets	2329:2344	In the third experiment, no interactions between fatty acid supplement and time of day were observed for intake, rumination, or rumen pH. Within all experiments, rumination fit or tended to fit a 24-h rhythm regardless of diet, with the amplitude of the rumination being reduced in low-starch diets and diets containing saturated FA or a mixture of saturated and unsaturated FA.
30316599	10	119	contain	containing	2356:2365	arg2	FA					2377:2378	saturated FA	2367:2378	saturated FA	2367:2378	In the third experiment, no interactions between fatty acid supplement and time of day were observed for intake, rumination, or rumen pH. Within all experiments, rumination fit or tended to fit a 24-h rhythm regardless of diet, with the amplitude of the rumination being reduced in low-starch diets and diets containing saturated FA or a mixture of saturated and unsaturated FA.
30316599	10	119	contain	containing	2356:2365	arg2	mixture					2385:2391	a mixture	2383:2391	a mixture of saturated and unsaturated FA	2383:2423	In the third experiment, no interactions between fatty acid supplement and time of day were observed for intake, rumination, or rumen pH. Within all experiments, rumination fit or tended to fit a 24-h rhythm regardless of diet, with the amplitude of the rumination being reduced in low-starch diets and diets containing saturated FA or a mixture of saturated and unsaturated FA.
30316599	10	120	theme	third	2054:2058	arg1	experiment					2060:2069	the third experiment	2050:2069	the third experiment	2050:2069	In the third experiment, no interactions between fatty acid supplement and time of day were observed for intake, rumination, or rumen pH. Within all experiments, rumination fit or tended to fit a 24-h rhythm regardless of diet, with the amplitude of the rumination being reduced in low-starch diets and diets containing saturated FA or a mixture of saturated and unsaturated FA.
30316599	4	121	theme	ground	1067:1072	arg1	corn					1088:1091	ground high-moisture corn	1067:1091	ground high-moisture corn	1067:1091	To investigate starch source and concentration in the second experiment, ground high-moisture corn or dry ground corn were fed in low- and high-starch diets (21 and 32%, respectively).
30316599	9	122	theme	rumination	1962:1971	arg1	patterns					1950:1957	the daily patterns	1940:1957	the daily patterns of rumination or rumen pH	1940:1983	Type of corn silage did not affect the daily patterns of rumination or rumen pH, but pH was reduced throughout the day in brown midrib diets.
30316599	0	123	theme	concentration	26:38	arg1	effects					4:10	The effects	0:10	The effects of source and concentration of dietary fiber, starch, and fatty acids on the daily patterns of feed intake, rumination, and rumen pH in dairy cows.	0:158	The effects of source and concentration of dietary fiber, starch, and fatty acids on the daily patterns of feed intake, rumination, and rumen pH in dairy cows.
30316599	3	124	theme	corn	918:921	arg1	silage					923:928	isogenic conventional corn silage	896:928	isogenic conventional corn silage	896:928	To investigate fiber content and digestibility in the first experiment, brown midrib or isogenic conventional corn silage were fed in low- and high-NDF diets (29 and 38%, respectively).
30316599	10	125	theme	fatty	2096:2100	arg1	supplement					2107:2116	fatty acid supplement	2096:2116	fatty acid supplement	2096:2116	In the third experiment, no interactions between fatty acid supplement and time of day were observed for intake, rumination, or rumen pH. Within all experiments, rumination fit or tended to fit a 24-h rhythm regardless of diet, with the amplitude of the rumination being reduced in low-starch diets and diets containing saturated FA or a mixture of saturated and unsaturated FA.
30316599	10	126	theme	FA	2422:2423	arg1	mixture					2385:2391	a mixture	2383:2391	a mixture of saturated and unsaturated FA	2383:2423	In the third experiment, no interactions between fatty acid supplement and time of day were observed for intake, rumination, or rumen pH. Within all experiments, rumination fit or tended to fit a 24-h rhythm regardless of diet, with the amplitude of the rumination being reduced in low-starch diets and diets containing saturated FA or a mixture of saturated and unsaturated FA.
30316599	10	126	theme	FA	2422:2423	arg1	FA					2377:2378	saturated FA	2367:2378	saturated FA	2367:2378	In the third experiment, no interactions between fatty acid supplement and time of day were observed for intake, rumination, or rumen pH. Within all experiments, rumination fit or tended to fit a 24-h rhythm regardless of diet, with the amplitude of the rumination being reduced in low-starch diets and diets containing saturated FA or a mixture of saturated and unsaturated FA.
30316599	5	127	theme	square	1294:1299	arg1	design					1301:1306	a replicated 4 × 4 Latin square design	1269:1306	a replicated 4 × 4 Latin square design that fed cows 1×/d at 0900 h	1269:1335	Effect of fat concentration and saturation was investigated in the third experiment using a replicated 4 × 4 Latin square design that fed cows 1×/d at 0900 h; treatments included a control diet with no added fat and 2.5% added saturated FA, unsaturated FA, or a mixture of the saturated and unsaturated FA.
30316599	10	128	theme	24-h	2243:2246	arg1	rhythm					2248:2253	a 24-h rhythm	2241:2253	a 24-h rhythm	2241:2253	In the third experiment, no interactions between fatty acid supplement and time of day were observed for intake, rumination, or rumen pH. Within all experiments, rumination fit or tended to fit a 24-h rhythm regardless of diet, with the amplitude of the rumination being reduced in low-starch diets and diets containing saturated FA or a mixture of saturated and unsaturated FA.
30316599	9	129	theme	rumen	1976:1980	arg1	pH					1982:1983	rumen pH	1976:1983	rumen pH	1976:1983	Type of corn silage did not affect the daily patterns of rumination or rumen pH, but pH was reduced throughout the day in brown midrib diets.
30316599	1	130	from	timing	253:258	arg1	cow					295:297	the dairy cow	285:297	the dairy cow	285:297	The daily patterns of feed intake and rumination influence rumen fermentation, rumen pH, and timing of absorbed nutrients in the dairy cow, but the effects of diet composition on these patterns are not well characterized.
30316599	8	131	theme	total	1822:1826	arg1	pH					1840:1841	total daily rumen pH	1822:1841	total daily rumen pH	1822:1841	High-starch diets decreased total daily rumen pH equally across the day, but did not change the daily pattern.
30316599	11	132	theme	daily	2477:2481	arg1	pattern					2483:2489	a daily pattern	2475:2489	a daily pattern that was minimally modified by dietary fiber and starch type and level or fat level and fatty acid profile	2475:2596	Overall, intake, rumination, and rumen pH follow a daily pattern that was minimally modified by dietary fiber and starch type and level or fat level and fatty acid profile.
30316599	8	133	theme	rumen	1834:1838	arg1	pH					1840:1841	total daily rumen pH	1822:1841	total daily rumen pH	1822:1841	High-starch diets decreased total daily rumen pH equally across the day, but did not change the daily pattern.
30316599	6	134	theme	similar	1532:1538	arg1	pattern					1546:1552	a similar daily pattern	1530:1552	a similar daily pattern regardless of starch and NDF concentration or digestibility	1530:1612	In the first 2 experiments, intake followed a similar daily pattern regardless of starch and NDF concentration or digestibility.
30316599	1	135	theme	daily	164:168	arg1	patterns					170:177	The daily patterns	160:177	The daily patterns of feed intake and rumination	160:207	The daily patterns of feed intake and rumination influence rumen fermentation, rumen pH, and timing of absorbed nutrients in the dairy cow, but the effects of diet composition on these patterns are not well characterized.
30316599	10	136	theme	day	2130:2132	arg1	time					2122:2125	time	2122:2125	time of day	2122:2132	In the third experiment, no interactions between fatty acid supplement and time of day were observed for intake, rumination, or rumen pH. Within all experiments, rumination fit or tended to fit a 24-h rhythm regardless of diet, with the amplitude of the rumination being reduced in low-starch diets and diets containing saturated FA or a mixture of saturated and unsaturated FA.
30316599	10	136	theme	day	2130:2132	arg1	supplement					2107:2116	fatty acid supplement	2096:2116	fatty acid supplement	2096:2116	In the third experiment, no interactions between fatty acid supplement and time of day were observed for intake, rumination, or rumen pH. Within all experiments, rumination fit or tended to fit a 24-h rhythm regardless of diet, with the amplitude of the rumination being reduced in low-starch diets and diets containing saturated FA or a mixture of saturated and unsaturated FA.
30316599	2	137	theme	square	688:693	arg1	designs					695:701	duplicated 4 × 4 Latin square designs	665:701	duplicated 4 × 4 Latin square designs	665:701	Data from 3 previously published experiments were examined to determine the influence of dietary starch, fiber, and fatty acids (FA) on daily patterns of intake, rumination, and rumen pH. Dietary neutral detergent fiber (NDF) and starch were investigated in 2 experiments, each with duplicated 4 × 4 Latin square designs with a 2 × 2 factorial arrangement of treatments in cows fed cows 1×/d at 1200 and 1400 h, respectively.
30316599	5	138	theme	unsaturated	1420:1430	arg1	FA					1432:1433	unsaturated FA	1420:1433	unsaturated FA	1420:1433	Effect of fat concentration and saturation was investigated in the third experiment using a replicated 4 × 4 Latin square design that fed cows 1×/d at 0900 h; treatments included a control diet with no added fat and 2.5% added saturated FA, unsaturated FA, or a mixture of the saturated and unsaturated FA.
30316599	4	139	theme	high-moisture	1074:1086	arg1	corn					1088:1091	ground high-moisture corn	1067:1091	ground high-moisture corn	1067:1091	To investigate starch source and concentration in the second experiment, ground high-moisture corn or dry ground corn were fed in low- and high-starch diets (21 and 32%, respectively).
30854603	4	0	theme	stem	596:599	arg1	cells					601:605	C-type natriuretic peptide (CNP) gene-modified bone marrow-derived mesenchymal stem cells	517:605	C-type natriuretic peptide (CNP) gene-modified bone marrow-derived mesenchymal stem cells (BMSCs)	517:613	In in vitro experiments, we obtained C-type natriuretic peptide (CNP) gene-modified bone marrow-derived mesenchymal stem cells (BMSCs) by transfection with recombinant adenovirus containing the CNP gene and revealed that CNP gene-modified BMSCs had good chondrogenic differentiation ability.
30854603	4	0	theme	stem	596:599	arg1	BMSCs					608:612	BMSCs	608:612	BMSCs	608:612	In in vitro experiments, we obtained C-type natriuretic peptide (CNP) gene-modified bone marrow-derived mesenchymal stem cells (BMSCs) by transfection with recombinant adenovirus containing the CNP gene and revealed that CNP gene-modified BMSCs had good chondrogenic differentiation ability.
30854603	5	1	dep	synthesized	817:827	arg1	had					884:886	had	884:886	had a suitable aperture size for chondrogenesis	884:930	By the freeze-drying method, we successfully synthesized a chitosan/silk fibroin (CS/SF) porous scaffold, which had a suitable aperture size for chondrogenesis.
30854603	4	2	theme	CNP	674:676	arg1	gene					678:681	the CNP gene	670:681	the CNP gene	670:681	In in vitro experiments, we obtained C-type natriuretic peptide (CNP) gene-modified bone marrow-derived mesenchymal stem cells (BMSCs) by transfection with recombinant adenovirus containing the CNP gene and revealed that CNP gene-modified BMSCs had good chondrogenic differentiation ability.
30854603	7	3	from	point	1287:1291	arg1	groups					1267:1272	the other three groups	1251:1272	the other three groups at each time point	1251:1291	The gross morphology and histology examination results showed that the composite of the CNP gene-modified BMSCs and CS/SF scaffolds had better repair effects than those of the other three groups at each time point.
30854603	7	4	theme	examination	1114:1124	arg1	results					1126:1132	The gross morphology and histology examination results	1079:1132	The gross morphology and histology examination results	1079:1132	The gross morphology and histology examination results showed that the composite of the CNP gene-modified BMSCs and CS/SF scaffolds had better repair effects than those of the other three groups at each time point.
30854603	7	5	contain	had	1211:1213	arg1	scaffolds					1201:1209	CS/SF scaffolds	1195:1209	CS/SF scaffolds	1195:1209	The gross morphology and histology examination results showed that the composite of the CNP gene-modified BMSCs and CS/SF scaffolds had better repair effects than those of the other three groups at each time point.
30854603	7	5	contain	had	1211:1213	arg1	BMSCs					1185:1189	the CNP gene-modified BMSCs	1163:1189	the CNP gene-modified BMSCs	1163:1189	The gross morphology and histology examination results showed that the composite of the CNP gene-modified BMSCs and CS/SF scaffolds had better repair effects than those of the other three groups at each time point.
30854603	7	5	contain	had	1211:1213	arg1	composite					1150:1158	the composite	1146:1158	the composite of the CNP gene-modified BMSCs and CS/SF scaffolds	1146:1209	The gross morphology and histology examination results showed that the composite of the CNP gene-modified BMSCs and CS/SF scaffolds had better repair effects than those of the other three groups at each time point.
30854603	7	5	contain	had	1211:1213	arg2	effects					1229:1235	better repair effects	1215:1235	better repair effects	1215:1235	The gross morphology and histology examination results showed that the composite of the CNP gene-modified BMSCs and CS/SF scaffolds had better repair effects than those of the other three groups at each time point.
30854603	1	6	theme	articular	207:215	arg1	defects					227:233	articular cartilage defects	207:233	articular cartilage defects	207:233	The treatment of articular cartilage defects has become a major clinical concern.
30854603	4	7	theme	recombinant	636:646	arg1	adenovirus					648:657	recombinant adenovirus	636:657	recombinant adenovirus containing the CNP gene	636:681	In in vitro experiments, we obtained C-type natriuretic peptide (CNP) gene-modified bone marrow-derived mesenchymal stem cells (BMSCs) by transfection with recombinant adenovirus containing the CNP gene and revealed that CNP gene-modified BMSCs had good chondrogenic differentiation ability.
30854603	5	8	theme	porous	861:866	arg1	scaffold					868:875	a chitosan/silk fibroin (CS/SF) porous scaffold	829:875	a chitosan/silk fibroin (CS/SF) porous scaffold	829:875	By the freeze-drying method, we successfully synthesized a chitosan/silk fibroin (CS/SF) porous scaffold, which had a suitable aperture size for chondrogenesis.
30854603	8	9	theme	CS/SF	1437:1441	arg1	scaffolds					1443:1451	CS/SF scaffolds	1437:1451	CS/SF scaffolds	1437:1451	Additionally, compared to the group with BMSCs and scaffolds, we found that there was more cartilage matrix in the CNP gene-modified BMSCs and CS/SF scaffolds group.
30854603	7	10	theme	gross	1083:1087	arg1	morphology					1089:1098	gross morphology	1083:1098	gross morphology	1083:1098	The gross morphology and histology examination results showed that the composite of the CNP gene-modified BMSCs and CS/SF scaffolds had better repair effects than those of the other three groups at each time point.
30854603	6	11	theme	gene-modified	953:965	arg1	BMSCs					967:971	CNP gene-modified BMSCs	949:971	CNP gene-modified BMSCs	949:971	Then, we loaded CNP gene-modified BMSCs onto CS/SF scaffolds and tested their effect on repairing full-thickness cartilage defects in rat joints.
30854603	0	12	theme	articular	156:164	arg1	cartilage					166:174	articular cartilage	156:174	articular cartilage regeneration	156:187	Integration of C-type natriuretic peptide gene-modified bone marrow mesenchymal stem cells with chitosan/silk fibroin scaffolds as a promising strategy for articular cartilage regeneration.
30854603	4	13	theme	chondrogenic	734:745	arg1	ability					763:769	good chondrogenic differentiation ability	729:769	good chondrogenic differentiation ability	729:769	In in vitro experiments, we obtained C-type natriuretic peptide (CNP) gene-modified bone marrow-derived mesenchymal stem cells (BMSCs) by transfection with recombinant adenovirus containing the CNP gene and revealed that CNP gene-modified BMSCs had good chondrogenic differentiation ability.
30854603	6	14	theme	cartilage	1046:1054	arg1	defects					1056:1062	full-thickness cartilage defects	1031:1062	full-thickness cartilage defects in rat joints	1031:1076	Then, we loaded CNP gene-modified BMSCs onto CS/SF scaffolds and tested their effect on repairing full-thickness cartilage defects in rat joints.
30854603	5	15	theme	freeze-drying	779:791	arg1	method					793:798	the freeze-drying method	775:798	the freeze-drying method	775:798	By the freeze-drying method, we successfully synthesized a chitosan/silk fibroin (CS/SF) porous scaffold, which had a suitable aperture size for chondrogenesis.
30854603	7	16	theme	CS/SF	1195:1199	arg1	scaffolds					1201:1209	CS/SF scaffolds	1195:1209	CS/SF scaffolds	1195:1209	The gross morphology and histology examination results showed that the composite of the CNP gene-modified BMSCs and CS/SF scaffolds had better repair effects than those of the other three groups at each time point.
30854603	4	17	theme	gene-modified	705:717	arg1	BMSCs					719:723	CNP gene-modified BMSCs	701:723	CNP gene-modified BMSCs	701:723	In in vitro experiments, we obtained C-type natriuretic peptide (CNP) gene-modified bone marrow-derived mesenchymal stem cells (BMSCs) by transfection with recombinant adenovirus containing the CNP gene and revealed that CNP gene-modified BMSCs had good chondrogenic differentiation ability.
30854603	0	18	theme	cells	85:89	arg1	Integration					0:10	Integration	0:10	Integration of C-type natriuretic peptide gene-modified bone marrow mesenchymal stem cells with chitosan/silk fibroin	0:116	Integration of C-type natriuretic peptide gene-modified bone marrow mesenchymal stem cells with chitosan/silk fibroin scaffolds as a promising strategy for articular cartilage regeneration.
30854603	9	19	theme	CNP	1525:1527	arg1	BMSCs					1543:1547	CNP gene-modified BMSCs	1525:1547	CNP gene-modified BMSCs	1525:1547	Data obtained in the present study suggest that the composite of CNP gene-modified BMSCs and CS/SF scaffolds represent promising strategies for repairing focal cartilage lesions.
30854603	6	20	from	defects	1056:1062	arg1	joints					1071:1076	rat joints	1067:1076	rat joints	1067:1076	Then, we loaded CNP gene-modified BMSCs onto CS/SF scaffolds and tested their effect on repairing full-thickness cartilage defects in rat joints.
30854603	0	21	theme	chitosan/silk	96:108	arg1	fibroin					110:116	chitosan/silk fibroin	96:116	chitosan/silk fibroin	96:116	Integration of C-type natriuretic peptide gene-modified bone marrow mesenchymal stem cells with chitosan/silk fibroin scaffolds as a promising strategy for articular cartilage regeneration.
30854603	9	22	theme	BMSCs	1543:1547	arg1	composite					1512:1520	the composite	1508:1520	the composite of CNP gene-modified BMSCs	1508:1547	Data obtained in the present study suggest that the composite of CNP gene-modified BMSCs and CS/SF scaffolds represent promising strategies for repairing focal cartilage lesions.
30854603	9	22	theme	BMSCs	1543:1547	arg1	BMSCs					1543:1547	CNP gene-modified BMSCs	1525:1547	CNP gene-modified BMSCs	1525:1547	Data obtained in the present study suggest that the composite of CNP gene-modified BMSCs and CS/SF scaffolds represent promising strategies for repairing focal cartilage lesions.
30854603	9	22	theme	BMSCs	1543:1547	arg1	scaffolds					1559:1567	CS/SF scaffolds	1553:1567	CS/SF scaffolds	1553:1567	Data obtained in the present study suggest that the composite of CNP gene-modified BMSCs and CS/SF scaffolds represent promising strategies for repairing focal cartilage lesions.
30854603	7	23	theme	repair	1222:1227	arg1	effects					1229:1235	better repair effects	1215:1235	better repair effects	1215:1235	The gross morphology and histology examination results showed that the composite of the CNP gene-modified BMSCs and CS/SF scaffolds had better repair effects than those of the other three groups at each time point.
30854603	7	24	theme	gene-modified	1171:1183	arg1	BMSCs					1185:1189	the CNP gene-modified BMSCs	1163:1189	the CNP gene-modified BMSCs	1163:1189	The gross morphology and histology examination results showed that the composite of the CNP gene-modified BMSCs and CS/SF scaffolds had better repair effects than those of the other three groups at each time point.
30854603	9	25	theme	CS/SF	1553:1557	arg1	scaffolds					1559:1567	CS/SF scaffolds	1553:1567	CS/SF scaffolds	1553:1567	Data obtained in the present study suggest that the composite of CNP gene-modified BMSCs and CS/SF scaffolds represent promising strategies for repairing focal cartilage lesions.
30854603	3	26	theme	gene	393:396	arg1	therapy					398:404	gene therapy	393:404	gene therapy with tissue engineering methods	393:436	In this work, we combined gene therapy with tissue engineering methods to test their effect on cartilage repair.
30854603	8	27	theme	more	1380:1383	arg1	matrix					1395:1400	more cartilage matrix	1380:1400	more cartilage matrix	1380:1400	Additionally, compared to the group with BMSCs and scaffolds, we found that there was more cartilage matrix in the CNP gene-modified BMSCs and CS/SF scaffolds group.
30854603	0	28	theme	C-type	15:20	arg1	peptide					34:40	C-type natriuretic peptide	15:40	C-type natriuretic peptide gene-modified bone marrow mesenchymal stem cells	15:89	Integration of C-type natriuretic peptide gene-modified bone marrow mesenchymal stem cells with chitosan/silk fibroin scaffolds as a promising strategy for articular cartilage regeneration.
30854603	4	29	dep	in	483:484	arg1	vitro					486:490	vitro	486:490	vitro	486:490	In in vitro experiments, we obtained C-type natriuretic peptide (CNP) gene-modified bone marrow-derived mesenchymal stem cells (BMSCs) by transfection with recombinant adenovirus containing the CNP gene and revealed that CNP gene-modified BMSCs had good chondrogenic differentiation ability.
30854603	3	30	theme	engineering	418:428	arg1	methods					430:436	tissue engineering methods	411:436	tissue engineering methods	411:436	In this work, we combined gene therapy with tissue engineering methods to test their effect on cartilage repair.
30854603	4	31	theme	bone	564:567	arg1	cells					601:605	C-type natriuretic peptide (CNP) gene-modified bone marrow-derived mesenchymal stem cells	517:605	C-type natriuretic peptide (CNP) gene-modified bone marrow-derived mesenchymal stem cells (BMSCs)	517:613	In in vitro experiments, we obtained C-type natriuretic peptide (CNP) gene-modified bone marrow-derived mesenchymal stem cells (BMSCs) by transfection with recombinant adenovirus containing the CNP gene and revealed that CNP gene-modified BMSCs had good chondrogenic differentiation ability.
30854603	4	31	theme	bone	564:567	arg1	BMSCs					608:612	BMSCs	608:612	BMSCs	608:612	In in vitro experiments, we obtained C-type natriuretic peptide (CNP) gene-modified bone marrow-derived mesenchymal stem cells (BMSCs) by transfection with recombinant adenovirus containing the CNP gene and revealed that CNP gene-modified BMSCs had good chondrogenic differentiation ability.
30854603	4	32	contain	containing	659:668	arg1	adenovirus					648:657	recombinant adenovirus	636:657	recombinant adenovirus containing the CNP gene	636:681	In in vitro experiments, we obtained C-type natriuretic peptide (CNP) gene-modified bone marrow-derived mesenchymal stem cells (BMSCs) by transfection with recombinant adenovirus containing the CNP gene and revealed that CNP gene-modified BMSCs had good chondrogenic differentiation ability.
30854603	4	32	contain	containing	659:668	arg2	gene					678:681	the CNP gene	670:681	the CNP gene	670:681	In in vitro experiments, we obtained C-type natriuretic peptide (CNP) gene-modified bone marrow-derived mesenchymal stem cells (BMSCs) by transfection with recombinant adenovirus containing the CNP gene and revealed that CNP gene-modified BMSCs had good chondrogenic differentiation ability.
30854603	0	33	theme	peptide	34:40	arg1	cells					85:89	C-type natriuretic peptide gene-modified bone marrow mesenchymal stem cells	15:89	C-type natriuretic peptide gene-modified bone marrow mesenchymal stem cells	15:89	Integration of C-type natriuretic peptide gene-modified bone marrow mesenchymal stem cells with chitosan/silk fibroin scaffolds as a promising strategy for articular cartilage regeneration.
30854603	7	34	theme	BMSCs	1185:1189	arg1	scaffolds					1201:1209	CS/SF scaffolds	1195:1209	CS/SF scaffolds	1195:1209	The gross morphology and histology examination results showed that the composite of the CNP gene-modified BMSCs and CS/SF scaffolds had better repair effects than those of the other three groups at each time point.
30854603	7	34	theme	BMSCs	1185:1189	arg1	BMSCs					1185:1189	the CNP gene-modified BMSCs	1163:1189	the CNP gene-modified BMSCs	1163:1189	The gross morphology and histology examination results showed that the composite of the CNP gene-modified BMSCs and CS/SF scaffolds had better repair effects than those of the other three groups at each time point.
30854603	7	34	theme	BMSCs	1185:1189	arg1	composite					1150:1158	the composite	1146:1158	the composite of the CNP gene-modified BMSCs and CS/SF scaffolds	1146:1209	The gross morphology and histology examination results showed that the composite of the CNP gene-modified BMSCs and CS/SF scaffolds had better repair effects than those of the other three groups at each time point.
30854603	4	35	theme	natriuretic	524:534	arg1	CNP					545:547	CNP	545:547	CNP	545:547	In in vitro experiments, we obtained C-type natriuretic peptide (CNP) gene-modified bone marrow-derived mesenchymal stem cells (BMSCs) by transfection with recombinant adenovirus containing the CNP gene and revealed that CNP gene-modified BMSCs had good chondrogenic differentiation ability.
30854603	4	35	theme	natriuretic	524:534	arg1	peptide					536:542	C-type natriuretic peptide	517:542	C-type natriuretic peptide (CNP) gene-modified bone marrow-derived mesenchymal stem cells (BMSCs)	517:613	In in vitro experiments, we obtained C-type natriuretic peptide (CNP) gene-modified bone marrow-derived mesenchymal stem cells (BMSCs) by transfection with recombinant adenovirus containing the CNP gene and revealed that CNP gene-modified BMSCs had good chondrogenic differentiation ability.
30854603	7	36	theme	other	1255:1259	arg1	groups					1267:1272	the other three groups	1251:1272	the other three groups at each time point	1251:1291	The gross morphology and histology examination results showed that the composite of the CNP gene-modified BMSCs and CS/SF scaffolds had better repair effects than those of the other three groups at each time point.
30854603	0	37	theme	bone	56:59	arg1	cells					85:89	C-type natriuretic peptide gene-modified bone marrow mesenchymal stem cells	15:89	C-type natriuretic peptide gene-modified bone marrow mesenchymal stem cells	15:89	Integration of C-type natriuretic peptide gene-modified bone marrow mesenchymal stem cells with chitosan/silk fibroin scaffolds as a promising strategy for articular cartilage regeneration.
30854603	9	38	theme	cartilage	1620:1628	arg1	lesions					1630:1636	focal cartilage lesions	1614:1636	focal cartilage lesions	1614:1636	Data obtained in the present study suggest that the composite of CNP gene-modified BMSCs and CS/SF scaffolds represent promising strategies for repairing focal cartilage lesions.
30854603	8	39	theme	gene-modified	1413:1425	arg1	BMSCs					1427:1431	CNP gene-modified BMSCs	1409:1431	CNP gene-modified BMSCs	1409:1431	Additionally, compared to the group with BMSCs and scaffolds, we found that there was more cartilage matrix in the CNP gene-modified BMSCs and CS/SF scaffolds group.
30854603	0	40	with	Integration	0:10	arg1	fibroin					110:116	chitosan/silk fibroin	96:116	chitosan/silk fibroin	96:116	Integration of C-type natriuretic peptide gene-modified bone marrow mesenchymal stem cells with chitosan/silk fibroin scaffolds as a promising strategy for articular cartilage regeneration.
30854603	8	41	with	group	1324:1328	arg1	scaffolds					1345:1353	scaffolds	1345:1353	scaffolds	1345:1353	Additionally, compared to the group with BMSCs and scaffolds, we found that there was more cartilage matrix in the CNP gene-modified BMSCs and CS/SF scaffolds group.
30854603	8	41	with	group	1324:1328	arg1	BMSCs					1335:1339	BMSCs	1335:1339	BMSCs	1335:1339	Additionally, compared to the group with BMSCs and scaffolds, we found that there was more cartilage matrix in the CNP gene-modified BMSCs and CS/SF scaffolds group.
30854603	1	42	theme	clinical	254:261	arg1	concern					263:269	a major clinical concern	246:269	a major clinical concern	246:269	The treatment of articular cartilage defects has become a major clinical concern.
30854603	4	43	link	marrow-derived	569:582	arg1	cells					601:605	C-type natriuretic peptide (CNP) gene-modified bone marrow-derived mesenchymal stem cells	517:605	C-type natriuretic peptide (CNP) gene-modified bone marrow-derived mesenchymal stem cells (BMSCs)	517:613	In in vitro experiments, we obtained C-type natriuretic peptide (CNP) gene-modified bone marrow-derived mesenchymal stem cells (BMSCs) by transfection with recombinant adenovirus containing the CNP gene and revealed that CNP gene-modified BMSCs had good chondrogenic differentiation ability.
30854603	4	43	link	marrow-derived	569:582	arg1	BMSCs					608:612	BMSCs	608:612	BMSCs	608:612	In in vitro experiments, we obtained C-type natriuretic peptide (CNP) gene-modified bone marrow-derived mesenchymal stem cells (BMSCs) by transfection with recombinant adenovirus containing the CNP gene and revealed that CNP gene-modified BMSCs had good chondrogenic differentiation ability.
30854603	3	44	from	effect	452:457	arg1	repair					472:477	cartilage repair	462:477	cartilage repair	462:477	In this work, we combined gene therapy with tissue engineering methods to test their effect on cartilage repair.
30854603	4	45	theme	mesenchymal	584:594	arg1	cells					601:605	C-type natriuretic peptide (CNP) gene-modified bone marrow-derived mesenchymal stem cells	517:605	C-type natriuretic peptide (CNP) gene-modified bone marrow-derived mesenchymal stem cells (BMSCs)	517:613	In in vitro experiments, we obtained C-type natriuretic peptide (CNP) gene-modified bone marrow-derived mesenchymal stem cells (BMSCs) by transfection with recombinant adenovirus containing the CNP gene and revealed that CNP gene-modified BMSCs had good chondrogenic differentiation ability.
30854603	4	45	theme	mesenchymal	584:594	arg1	BMSCs					608:612	BMSCs	608:612	BMSCs	608:612	In in vitro experiments, we obtained C-type natriuretic peptide (CNP) gene-modified bone marrow-derived mesenchymal stem cells (BMSCs) by transfection with recombinant adenovirus containing the CNP gene and revealed that CNP gene-modified BMSCs had good chondrogenic differentiation ability.
30854603	9	46	theme	focal	1614:1618	arg1	lesions					1630:1636	focal cartilage lesions	1614:1636	focal cartilage lesions	1614:1636	Data obtained in the present study suggest that the composite of CNP gene-modified BMSCs and CS/SF scaffolds represent promising strategies for repairing focal cartilage lesions.
30854603	7	47	theme	CNP	1167:1169	arg1	BMSCs					1185:1189	the CNP gene-modified BMSCs	1163:1189	the CNP gene-modified BMSCs	1163:1189	The gross morphology and histology examination results showed that the composite of the CNP gene-modified BMSCs and CS/SF scaffolds had better repair effects than those of the other three groups at each time point.
30854603	4	48	contain	had	725:727	arg2	ability					763:769	good chondrogenic differentiation ability	729:769	good chondrogenic differentiation ability	729:769	In in vitro experiments, we obtained C-type natriuretic peptide (CNP) gene-modified bone marrow-derived mesenchymal stem cells (BMSCs) by transfection with recombinant adenovirus containing the CNP gene and revealed that CNP gene-modified BMSCs had good chondrogenic differentiation ability.
30854603	4	48	contain	had	725:727	arg1	BMSCs					719:723	CNP gene-modified BMSCs	701:723	CNP gene-modified BMSCs	701:723	In in vitro experiments, we obtained C-type natriuretic peptide (CNP) gene-modified bone marrow-derived mesenchymal stem cells (BMSCs) by transfection with recombinant adenovirus containing the CNP gene and revealed that CNP gene-modified BMSCs had good chondrogenic differentiation ability.
30854603	7	49	theme	histology	1104:1112	arg1	examination					1114:1124	histology examination	1104:1124	histology examination	1104:1124	The gross morphology and histology examination results showed that the composite of the CNP gene-modified BMSCs and CS/SF scaffolds had better repair effects than those of the other three groups at each time point.
30854603	7	50	theme	morphology	1089:1098	arg1	results					1126:1132	The gross morphology and histology examination results	1079:1132	The gross morphology and histology examination results	1079:1132	The gross morphology and histology examination results showed that the composite of the CNP gene-modified BMSCs and CS/SF scaffolds had better repair effects than those of the other three groups at each time point.
30854603	2	51	theme	effective	327:335	arg1	methods					337:343	effective methods	327:343	effective methods	327:343	Currently, additional efforts are necessary to develop effective methods to cure this disease.
30854603	1	52	theme	cartilage	217:225	arg1	defects					227:233	articular cartilage defects	207:233	articular cartilage defects	207:233	The treatment of articular cartilage defects has become a major clinical concern.
30854603	6	53	theme	CS/SF	978:982	arg1	scaffolds					984:992	CS/SF scaffolds	978:992	CS/SF scaffolds	978:992	Then, we loaded CNP gene-modified BMSCs onto CS/SF scaffolds and tested their effect on repairing full-thickness cartilage defects in rat joints.
30854603	8	54	theme	scaffolds	1443:1451	arg1	group					1453:1457	the CNP gene-modified BMSCs and CS/SF scaffolds group	1405:1457	the CNP gene-modified BMSCs and CS/SF scaffolds group	1405:1457	Additionally, compared to the group with BMSCs and scaffolds, we found that there was more cartilage matrix in the CNP gene-modified BMSCs and CS/SF scaffolds group.
30854603	3	55	with	therapy	398:404	arg1	methods					430:436	tissue engineering methods	411:436	tissue engineering methods	411:436	In this work, we combined gene therapy with tissue engineering methods to test their effect on cartilage repair.
30854603	4	56	with	transfection	618:629	arg1	adenovirus					648:657	recombinant adenovirus	636:657	recombinant adenovirus containing the CNP gene	636:681	In in vitro experiments, we obtained C-type natriuretic peptide (CNP) gene-modified bone marrow-derived mesenchymal stem cells (BMSCs) by transfection with recombinant adenovirus containing the CNP gene and revealed that CNP gene-modified BMSCs had good chondrogenic differentiation ability.
30854603	5	57	theme	suitable	890:897	arg1	size					908:911	a suitable aperture size	888:911	a suitable aperture size for chondrogenesis	888:930	By the freeze-drying method, we successfully synthesized a chitosan/silk fibroin (CS/SF) porous scaffold, which had a suitable aperture size for chondrogenesis.
30854603	0	58	theme	cartilage	166:174	arg1	regeneration					176:187	articular cartilage regeneration	156:187	articular cartilage regeneration	156:187	Integration of C-type natriuretic peptide gene-modified bone marrow mesenchymal stem cells with chitosan/silk fibroin scaffolds as a promising strategy for articular cartilage regeneration.
30854603	9	59	theme	present	1481:1487	arg1	study					1489:1493	the present study	1477:1493	the present study	1477:1493	Data obtained in the present study suggest that the composite of CNP gene-modified BMSCs and CS/SF scaffolds represent promising strategies for repairing focal cartilage lesions.
30854603	6	60	theme	CNP	949:951	arg1	BMSCs					967:971	CNP gene-modified BMSCs	949:971	CNP gene-modified BMSCs	949:971	Then, we loaded CNP gene-modified BMSCs onto CS/SF scaffolds and tested their effect on repairing full-thickness cartilage defects in rat joints.
30854603	0	61	theme	promising	133:141	arg1	strategy					143:150	a promising strategy	131:150	a promising strategy for articular cartilage regeneration	131:187	Integration of C-type natriuretic peptide gene-modified bone marrow mesenchymal stem cells with chitosan/silk fibroin scaffolds as a promising strategy for articular cartilage regeneration.
30854603	2	62	theme	additional	283:292	arg1	efforts					294:300	additional efforts	283:300	additional efforts	283:300	Currently, additional efforts are necessary to develop effective methods to cure this disease.
30854603	4	63	theme	good	729:732	arg1	ability					763:769	good chondrogenic differentiation ability	729:769	good chondrogenic differentiation ability	729:769	In in vitro experiments, we obtained C-type natriuretic peptide (CNP) gene-modified bone marrow-derived mesenchymal stem cells (BMSCs) by transfection with recombinant adenovirus containing the CNP gene and revealed that CNP gene-modified BMSCs had good chondrogenic differentiation ability.
30854603	0	64	theme	stem	80:83	arg1	cells					85:89	C-type natriuretic peptide gene-modified bone marrow mesenchymal stem cells	15:89	C-type natriuretic peptide gene-modified bone marrow mesenchymal stem cells	15:89	Integration of C-type natriuretic peptide gene-modified bone marrow mesenchymal stem cells with chitosan/silk fibroin scaffolds as a promising strategy for articular cartilage regeneration.
30854603	5	65	theme	aperture	899:906	arg1	size					908:911	a suitable aperture size	888:911	a suitable aperture size for chondrogenesis	888:930	By the freeze-drying method, we successfully synthesized a chitosan/silk fibroin (CS/SF) porous scaffold, which had a suitable aperture size for chondrogenesis.
30854603	6	66	theme	full-thickness	1031:1044	arg1	defects					1056:1062	full-thickness cartilage defects	1031:1062	full-thickness cartilage defects in rat joints	1031:1076	Then, we loaded CNP gene-modified BMSCs onto CS/SF scaffolds and tested their effect on repairing full-thickness cartilage defects in rat joints.
30854603	4	67	theme	C-type	517:522	arg1	CNP					545:547	CNP	545:547	CNP	545:547	In in vitro experiments, we obtained C-type natriuretic peptide (CNP) gene-modified bone marrow-derived mesenchymal stem cells (BMSCs) by transfection with recombinant adenovirus containing the CNP gene and revealed that CNP gene-modified BMSCs had good chondrogenic differentiation ability.
30854603	4	67	theme	C-type	517:522	arg1	peptide					536:542	C-type natriuretic peptide	517:542	C-type natriuretic peptide (CNP) gene-modified bone marrow-derived mesenchymal stem cells (BMSCs)	517:613	In in vitro experiments, we obtained C-type natriuretic peptide (CNP) gene-modified bone marrow-derived mesenchymal stem cells (BMSCs) by transfection with recombinant adenovirus containing the CNP gene and revealed that CNP gene-modified BMSCs had good chondrogenic differentiation ability.
30854603	4	68	theme	CNP	701:703	arg1	BMSCs					719:723	CNP gene-modified BMSCs	701:723	CNP gene-modified BMSCs	701:723	In in vitro experiments, we obtained C-type natriuretic peptide (CNP) gene-modified bone marrow-derived mesenchymal stem cells (BMSCs) by transfection with recombinant adenovirus containing the CNP gene and revealed that CNP gene-modified BMSCs had good chondrogenic differentiation ability.
30854603	9	69	theme	gene-modified	1529:1541	arg1	BMSCs					1543:1547	CNP gene-modified BMSCs	1525:1547	CNP gene-modified BMSCs	1525:1547	Data obtained in the present study suggest that the composite of CNP gene-modified BMSCs and CS/SF scaffolds represent promising strategies for repairing focal cartilage lesions.
30854603	4	70	theme	in	483:484	arg1	experiments					492:502	in vitro experiments	483:502	in vitro experiments	483:502	In in vitro experiments, we obtained C-type natriuretic peptide (CNP) gene-modified bone marrow-derived mesenchymal stem cells (BMSCs) by transfection with recombinant adenovirus containing the CNP gene and revealed that CNP gene-modified BMSCs had good chondrogenic differentiation ability.
30854603	5	71	contain	had	884:886	arg2	size					908:911	a suitable aperture size	888:911	a suitable aperture size for chondrogenesis	888:930	By the freeze-drying method, we successfully synthesized a chitosan/silk fibroin (CS/SF) porous scaffold, which had a suitable aperture size for chondrogenesis.
30854603	5	71	contain	had	884:886	arg1	which					878:882	which	878:882	which	878:882	By the freeze-drying method, we successfully synthesized a chitosan/silk fibroin (CS/SF) porous scaffold, which had a suitable aperture size for chondrogenesis.
30854603	7	72	theme	better	1215:1220	arg1	effects					1229:1235	better repair effects	1215:1235	better repair effects	1215:1235	The gross morphology and histology examination results showed that the composite of the CNP gene-modified BMSCs and CS/SF scaffolds had better repair effects than those of the other three groups at each time point.
30854603	1	73	theme	defects	227:233	arg1	treatment					194:202	The treatment	190:202	The treatment of articular cartilage defects	190:233	The treatment of articular cartilage defects has become a major clinical concern.
30854603	7	74	theme	scaffolds	1201:1209	arg1	scaffolds					1201:1209	CS/SF scaffolds	1195:1209	CS/SF scaffolds	1195:1209	The gross morphology and histology examination results showed that the composite of the CNP gene-modified BMSCs and CS/SF scaffolds had better repair effects than those of the other three groups at each time point.
30854603	7	74	theme	scaffolds	1201:1209	arg1	BMSCs					1185:1189	the CNP gene-modified BMSCs	1163:1189	the CNP gene-modified BMSCs	1163:1189	The gross morphology and histology examination results showed that the composite of the CNP gene-modified BMSCs and CS/SF scaffolds had better repair effects than those of the other three groups at each time point.
30854603	7	74	theme	scaffolds	1201:1209	arg1	composite					1150:1158	the composite	1146:1158	the composite of the CNP gene-modified BMSCs and CS/SF scaffolds	1146:1209	The gross morphology and histology examination results showed that the composite of the CNP gene-modified BMSCs and CS/SF scaffolds had better repair effects than those of the other three groups at each time point.
30854603	4	75	theme	gene-modified	550:562	arg1	cells					601:605	C-type natriuretic peptide (CNP) gene-modified bone marrow-derived mesenchymal stem cells	517:605	C-type natriuretic peptide (CNP) gene-modified bone marrow-derived mesenchymal stem cells (BMSCs)	517:613	In in vitro experiments, we obtained C-type natriuretic peptide (CNP) gene-modified bone marrow-derived mesenchymal stem cells (BMSCs) by transfection with recombinant adenovirus containing the CNP gene and revealed that CNP gene-modified BMSCs had good chondrogenic differentiation ability.
30854603	4	75	theme	gene-modified	550:562	arg1	BMSCs					608:612	BMSCs	608:612	BMSCs	608:612	In in vitro experiments, we obtained C-type natriuretic peptide (CNP) gene-modified bone marrow-derived mesenchymal stem cells (BMSCs) by transfection with recombinant adenovirus containing the CNP gene and revealed that CNP gene-modified BMSCs had good chondrogenic differentiation ability.
30854603	4	76	theme	differentiation	747:761	arg1	ability					763:769	good chondrogenic differentiation ability	729:769	good chondrogenic differentiation ability	729:769	In in vitro experiments, we obtained C-type natriuretic peptide (CNP) gene-modified bone marrow-derived mesenchymal stem cells (BMSCs) by transfection with recombinant adenovirus containing the CNP gene and revealed that CNP gene-modified BMSCs had good chondrogenic differentiation ability.
30854603	0	77	theme	natriuretic	22:32	arg1	peptide					34:40	C-type natriuretic peptide	15:40	C-type natriuretic peptide gene-modified bone marrow mesenchymal stem cells	15:89	Integration of C-type natriuretic peptide gene-modified bone marrow mesenchymal stem cells with chitosan/silk fibroin scaffolds as a promising strategy for articular cartilage regeneration.
30854603	9	78	theme	promising	1579:1587	arg1	strategies					1589:1598	promising strategies	1579:1598	promising strategies for repairing focal cartilage lesions	1579:1636	Data obtained in the present study suggest that the composite of CNP gene-modified BMSCs and CS/SF scaffolds represent promising strategies for repairing focal cartilage lesions.
30854603	4	79	theme	marrow-derived	569:582	arg1	cells					601:605	C-type natriuretic peptide (CNP) gene-modified bone marrow-derived mesenchymal stem cells	517:605	C-type natriuretic peptide (CNP) gene-modified bone marrow-derived mesenchymal stem cells (BMSCs)	517:613	In in vitro experiments, we obtained C-type natriuretic peptide (CNP) gene-modified bone marrow-derived mesenchymal stem cells (BMSCs) by transfection with recombinant adenovirus containing the CNP gene and revealed that CNP gene-modified BMSCs had good chondrogenic differentiation ability.
30854603	4	79	theme	marrow-derived	569:582	arg1	BMSCs					608:612	BMSCs	608:612	BMSCs	608:612	In in vitro experiments, we obtained C-type natriuretic peptide (CNP) gene-modified bone marrow-derived mesenchymal stem cells (BMSCs) by transfection with recombinant adenovirus containing the CNP gene and revealed that CNP gene-modified BMSCs had good chondrogenic differentiation ability.
30854603	0	80	theme	gene-modified	42:54	arg1	cells					85:89	C-type natriuretic peptide gene-modified bone marrow mesenchymal stem cells	15:89	C-type natriuretic peptide gene-modified bone marrow mesenchymal stem cells	15:89	Integration of C-type natriuretic peptide gene-modified bone marrow mesenchymal stem cells with chitosan/silk fibroin scaffolds as a promising strategy for articular cartilage regeneration.
30854603	6	81	theme	rat	1067:1069	arg1	joints					1071:1076	rat joints	1067:1076	rat joints	1067:1076	Then, we loaded CNP gene-modified BMSCs onto CS/SF scaffolds and tested their effect on repairing full-thickness cartilage defects in rat joints.
30854603	4	82	theme	peptide	536:542	arg1	cells					601:605	C-type natriuretic peptide (CNP) gene-modified bone marrow-derived mesenchymal stem cells	517:605	C-type natriuretic peptide (CNP) gene-modified bone marrow-derived mesenchymal stem cells (BMSCs)	517:613	In in vitro experiments, we obtained C-type natriuretic peptide (CNP) gene-modified bone marrow-derived mesenchymal stem cells (BMSCs) by transfection with recombinant adenovirus containing the CNP gene and revealed that CNP gene-modified BMSCs had good chondrogenic differentiation ability.
30854603	4	82	theme	peptide	536:542	arg1	BMSCs					608:612	BMSCs	608:612	BMSCs	608:612	In in vitro experiments, we obtained C-type natriuretic peptide (CNP) gene-modified bone marrow-derived mesenchymal stem cells (BMSCs) by transfection with recombinant adenovirus containing the CNP gene and revealed that CNP gene-modified BMSCs had good chondrogenic differentiation ability.
30854603	8	83	theme	cartilage	1385:1393	arg1	matrix					1395:1400	more cartilage matrix	1380:1400	more cartilage matrix	1380:1400	Additionally, compared to the group with BMSCs and scaffolds, we found that there was more cartilage matrix in the CNP gene-modified BMSCs and CS/SF scaffolds group.
30854603	3	84	theme	cartilage	462:470	arg1	repair					472:477	cartilage repair	462:477	cartilage repair	462:477	In this work, we combined gene therapy with tissue engineering methods to test their effect on cartilage repair.
30854603	0	85	theme	marrow	61:66	arg1	cells					85:89	C-type natriuretic peptide gene-modified bone marrow mesenchymal stem cells	15:89	C-type natriuretic peptide gene-modified bone marrow mesenchymal stem cells	15:89	Integration of C-type natriuretic peptide gene-modified bone marrow mesenchymal stem cells with chitosan/silk fibroin scaffolds as a promising strategy for articular cartilage regeneration.
30854603	0	86	theme	mesenchymal	68:78	arg1	cells					85:89	C-type natriuretic peptide gene-modified bone marrow mesenchymal stem cells	15:89	C-type natriuretic peptide gene-modified bone marrow mesenchymal stem cells	15:89	Integration of C-type natriuretic peptide gene-modified bone marrow mesenchymal stem cells with chitosan/silk fibroin scaffolds as a promising strategy for articular cartilage regeneration.
30854603	3	87	theme	tissue	411:416	arg1	methods					430:436	tissue engineering methods	411:436	tissue engineering methods	411:436	In this work, we combined gene therapy with tissue engineering methods to test their effect on cartilage repair.
30854603	7	88	theme	time	1282:1285	arg1	point					1287:1291	each time point	1277:1291	each time point	1277:1291	The gross morphology and histology examination results showed that the composite of the CNP gene-modified BMSCs and CS/SF scaffolds had better repair effects than those of the other three groups at each time point.
30854603	8	89	theme	CNP	1409:1411	arg1	BMSCs					1427:1431	CNP gene-modified BMSCs	1409:1431	CNP gene-modified BMSCs	1409:1431	Additionally, compared to the group with BMSCs and scaffolds, we found that there was more cartilage matrix in the CNP gene-modified BMSCs and CS/SF scaffolds group.
30854603	1	90	theme	major	248:252	arg1	concern					263:269	a major clinical concern	246:269	a major clinical concern	246:269	The treatment of articular cartilage defects has become a major clinical concern.
30854603	8	91	theme	BMSCs	1427:1431	arg1	group					1453:1457	the CNP gene-modified BMSCs and CS/SF scaffolds group	1405:1457	the CNP gene-modified BMSCs and CS/SF scaffolds group	1405:1457	Additionally, compared to the group with BMSCs and scaffolds, we found that there was more cartilage matrix in the CNP gene-modified BMSCs and CS/SF scaffolds group.
31870956	8	0	dep	2 days	1179:1184	arg1	up					1173:1174	up	1173:1174	up	1173:1174	In comparison to Pluronic® micelles that released ferulic acid rapidly, micelle-nanogel composites sustained the release up to 2 days.
31870956	7	1	theme	excised	1028:1034	arg1	corneas					1043:1049	excised bovine corneas	1028:1049	excised bovine corneas	1028:1049	The formulations were characterized in terms of rheological behavior, biocompatibility, wound healing properties, ferulic acid release pattern and penetration into excised bovine corneas.
31870956	2	2	theme	low	336:338	arg1	stability					340:348	low stability	336:348	low stability	336:348	However, ocular bioavailability of efficient antioxidants (e.g. ferulic acid) after topical administration is limited by their poor solubility, low stability and short residence time.
31870956	9	3	dep	in	1240:1241	arg1	vitro					1243:1247	vitro	1243:1247	vitro	1243:1247	Furthermore, the micelle-nanogel formulation favored in vitro wound closure promoting fibroblasts growth and ex vivo accumulation of ferulic acid into both healthy and damaged corneas (>100 µg/cm2).
31870956	4	4	theme	ferulic	556:562	arg1	acid					564:567	ferulic acid	556:567	ferulic acid	556:567	Solubility enhancement factor of ferulic acid was found to be equal to 1.9 ± 0.3 and 3.4 ± 0.3 for 50 and 100 mg/ml Pluronic® F68 micellar solutions.
31870956	7	5	theme	wound	952:956	arg1	healing					958:964	wound healing	952:964	wound healing properties	952:975	The formulations were characterized in terms of rheological behavior, biocompatibility, wound healing properties, ferulic acid release pattern and penetration into excised bovine corneas.
31870956	4	6	theme	micellar	653:660	arg1	solutions					662:670	50 and 100 mg/ml Pluronic® F68 micellar solutions	622:670	50 and 100 mg/ml Pluronic® F68 micellar solutions	622:670	Solubility enhancement factor of ferulic acid was found to be equal to 1.9 ± 0.3 and 3.4 ± 0.3 for 50 and 100 mg/ml Pluronic® F68 micellar solutions.
31870956	9	7	dep	ex	1296:1297	arg1	vivo					1299:1302	vivo	1299:1302	vivo	1299:1302	Furthermore, the micelle-nanogel formulation favored in vitro wound closure promoting fibroblasts growth and ex vivo accumulation of ferulic acid into both healthy and damaged corneas (>100 µg/cm2).
31870956	9	8	theme	wound	1249:1253	arg1	closure					1255:1261	in vitro wound closure	1240:1261	in vitro wound closure promoting fibroblasts growth and ex vivo accumulation of ferulic acid into both healthy and damaged corneas (>100 µg/cm2)	1240:1383	Furthermore, the micelle-nanogel formulation favored in vitro wound closure promoting fibroblasts growth and ex vivo accumulation of ferulic acid into both healthy and damaged corneas (>100 µg/cm2).
31870956	9	9	theme	ferulic	1320:1326	arg1	acid					1328:1331	ferulic acid	1320:1331	ferulic acid	1320:1331	Furthermore, the micelle-nanogel formulation favored in vitro wound closure promoting fibroblasts growth and ex vivo accumulation of ferulic acid into both healthy and damaged corneas (>100 µg/cm2).
31870956	6	10	theme	Hyaluronan	778:787	arg1	nanogels					789:796	Hyaluronan nanogels	778:796	Hyaluronan nanogels	778:796	Hyaluronan nanogels showed dimensions of ~300 nm with positive zeta potential values.
31870956	1	11	theme	Corneal	59:65	arg1	healing					73:79	Corneal wound healing	59:79	Corneal wound healing after a trauma or a chemical injury	59:115	Corneal wound healing after a trauma or a chemical injury has been shown to correlate with antioxidant levels at the ocular surface.
31870956	9	12	dep	fibroblasts	1273:1283	arg1	growth					1285:1290	growth	1285:1290	growth	1285:1290	Furthermore, the micelle-nanogel formulation favored in vitro wound closure promoting fibroblasts growth and ex vivo accumulation of ferulic acid into both healthy and damaged corneas (>100 µg/cm2).
31870956	9	12	dep	fibroblasts	1273:1283	arg1	accumulation					1304:1315	ex vivo accumulation	1296:1315	ex vivo accumulation of ferulic acid into both healthy and damaged corneas (>100 µg/cm2)	1296:1383	Furthermore, the micelle-nanogel formulation favored in vitro wound closure promoting fibroblasts growth and ex vivo accumulation of ferulic acid into both healthy and damaged corneas (>100 µg/cm2).
31870956	9	12	dep	fibroblasts	1273:1283	arg1	fibroblasts					1273:1283	fibroblasts growth and ex vivo accumulation of ferulic acid into both healthy and damaged corneas (>100 µg/cm2)	1273:1383	fibroblasts growth and ex vivo accumulation of ferulic acid into both healthy and damaged corneas (>100 µg/cm2)	1273:1383	Furthermore, the micelle-nanogel formulation favored in vitro wound closure promoting fibroblasts growth and ex vivo accumulation of ferulic acid into both healthy and damaged corneas (>100 µg/cm2).
31870956	5	13	theme	blank	697:701	arg1	micelles					727:734	blank and ferulic acid loaded micelles	697:734	blank and ferulic acid loaded micelles	697:734	Hyaluronan was added to blank and ferulic acid loaded micelles, and then cross-linked with ε-polylysine.
31870956	9	14	theme	acid	1328:1331	arg1	growth					1285:1290	growth	1285:1290	growth	1285:1290	Furthermore, the micelle-nanogel formulation favored in vitro wound closure promoting fibroblasts growth and ex vivo accumulation of ferulic acid into both healthy and damaged corneas (>100 µg/cm2).
31870956	9	14	theme	acid	1328:1331	arg1	accumulation					1304:1315	ex vivo accumulation	1296:1315	ex vivo accumulation of ferulic acid into both healthy and damaged corneas (>100 µg/cm2)	1296:1383	Furthermore, the micelle-nanogel formulation favored in vitro wound closure promoting fibroblasts growth and ex vivo accumulation of ferulic acid into both healthy and damaged corneas (>100 µg/cm2).
31870956	9	14	theme	acid	1328:1331	arg1	fibroblasts					1273:1283	fibroblasts growth and ex vivo accumulation of ferulic acid into both healthy and damaged corneas (>100 µg/cm2)	1273:1383	fibroblasts growth and ex vivo accumulation of ferulic acid into both healthy and damaged corneas (>100 µg/cm2)	1273:1383	Furthermore, the micelle-nanogel formulation favored in vitro wound closure promoting fibroblasts growth and ex vivo accumulation of ferulic acid into both healthy and damaged corneas (>100 µg/cm2).
31870956	7	15	theme	penetration	1011:1021	arg1	terms					903:907	terms	903:907	terms of rheological behavior, biocompatibility, wound healing properties, ferulic acid release pattern and penetration into excised bovine corneas	903:1049	The formulations were characterized in terms of rheological behavior, biocompatibility, wound healing properties, ferulic acid release pattern and penetration into excised bovine corneas.
31870956	7	16	theme	biocompatibility	934:949	arg1	terms					903:907	terms	903:907	terms of rheological behavior, biocompatibility, wound healing properties, ferulic acid release pattern and penetration into excised bovine corneas	903:1049	The formulations were characterized in terms of rheological behavior, biocompatibility, wound healing properties, ferulic acid release pattern and penetration into excised bovine corneas.
31870956	1	17	theme	wound	67:71	arg1	healing					73:79	Corneal wound healing	59:79	Corneal wound healing after a trauma or a chemical injury	59:115	Corneal wound healing after a trauma or a chemical injury has been shown to correlate with antioxidant levels at the ocular surface.
31870956	4	18	theme	acid	564:567	arg1	factor					546:551	Solubility enhancement factor	523:551	Solubility enhancement factor of ferulic acid	523:567	Solubility enhancement factor of ferulic acid was found to be equal to 1.9 ± 0.3 and 3.4 ± 0.3 for 50 and 100 mg/ml Pluronic® F68 micellar solutions.
31870956	3	19	theme	efficient	493:501	arg1	delivery					503:510	efficient delivery	493:510	efficient delivery to cornea	493:520	The aim of this work was to formulate ferulic acid in a nanocomposite platform composed of nanogels and micelles for efficient delivery to cornea.
31870956	2	20	theme	residence	360:368	arg1	time					370:373	short residence time	354:373	short residence time	354:373	However, ocular bioavailability of efficient antioxidants (e.g. ferulic acid) after topical administration is limited by their poor solubility, low stability and short residence time.
31870956	1	21	theme	antioxidant	150:160	arg1	levels					162:167	antioxidant levels	150:167	antioxidant levels	150:167	Corneal wound healing after a trauma or a chemical injury has been shown to correlate with antioxidant levels at the ocular surface.
31870956	5	22	theme	ferulic	707:713	arg1	acid					715:718	ferulic acid	707:718	ferulic acid	707:718	Hyaluronan was added to blank and ferulic acid loaded micelles, and then cross-linked with ε-polylysine.
31870956	7	23	theme	behavior	924:931	arg1	terms					903:907	terms	903:907	terms of rheological behavior, biocompatibility, wound healing properties, ferulic acid release pattern and penetration into excised bovine corneas	903:1049	The formulations were characterized in terms of rheological behavior, biocompatibility, wound healing properties, ferulic acid release pattern and penetration into excised bovine corneas.
31870956	2	24	theme	ferulic	256:262	arg1	acid					264:267	ferulic acid	256:267	ferulic acid	256:267	However, ocular bioavailability of efficient antioxidants (e.g. ferulic acid) after topical administration is limited by their poor solubility, low stability and short residence time.
31870956	9	25	theme	healthy	1343:1349	arg1	corneas					1363:1369	both healthy and damaged corneas	1338:1369	both healthy and damaged corneas (>100 µg/cm2)	1338:1383	Furthermore, the micelle-nanogel formulation favored in vitro wound closure promoting fibroblasts growth and ex vivo accumulation of ferulic acid into both healthy and damaged corneas (>100 µg/cm2).
31870956	9	25	theme	healthy	1343:1349	arg1	>100 µg/cm2					1372:1382	>100 µg/cm2	1372:1382	>100 µg/cm2	1372:1382	Furthermore, the micelle-nanogel formulation favored in vitro wound closure promoting fibroblasts growth and ex vivo accumulation of ferulic acid into both healthy and damaged corneas (>100 µg/cm2).
31870956	3	26	theme	work	392:395	arg1	aim					380:382	The aim	376:382	The aim of this work	376:395	The aim of this work was to formulate ferulic acid in a nanocomposite platform composed of nanogels and micelles for efficient delivery to cornea.
31870956	4	27	theme	Solubility	523:532	arg1	factor					546:551	Solubility enhancement factor	523:551	Solubility enhancement factor of ferulic acid	523:567	Solubility enhancement factor of ferulic acid was found to be equal to 1.9 ± 0.3 and 3.4 ± 0.3 for 50 and 100 mg/ml Pluronic® F68 micellar solutions.
31870956	2	28	theme	short	354:358	arg1	time					370:373	short residence time	354:373	short residence time	354:373	However, ocular bioavailability of efficient antioxidants (e.g. ferulic acid) after topical administration is limited by their poor solubility, low stability and short residence time.
31870956	0	29	theme	ferulic	29:35	arg1	delivery					49:56	ferulic acid ocular delivery	29:56	ferulic acid ocular delivery	29:56	Micelle-nanogel platform for ferulic acid ocular delivery.
31870956	9	30	theme	damaged	1355:1361	arg1	corneas					1363:1369	both healthy and damaged corneas	1338:1369	both healthy and damaged corneas (>100 µg/cm2)	1338:1383	Furthermore, the micelle-nanogel formulation favored in vitro wound closure promoting fibroblasts growth and ex vivo accumulation of ferulic acid into both healthy and damaged corneas (>100 µg/cm2).
31870956	9	30	theme	damaged	1355:1361	arg1	>100 µg/cm2					1372:1382	>100 µg/cm2	1372:1382	>100 µg/cm2	1372:1382	Furthermore, the micelle-nanogel formulation favored in vitro wound closure promoting fibroblasts growth and ex vivo accumulation of ferulic acid into both healthy and damaged corneas (>100 µg/cm2).
31870956	7	31	theme	rheological	912:922	arg1	behavior					924:931	rheological behavior	912:931	rheological behavior	912:931	The formulations were characterized in terms of rheological behavior, biocompatibility, wound healing properties, ferulic acid release pattern and penetration into excised bovine corneas.
31870956	1	32	theme	ocular	176:181	arg1	surface					183:189	the ocular surface	172:189	the ocular surface	172:189	Corneal wound healing after a trauma or a chemical injury has been shown to correlate with antioxidant levels at the ocular surface.
31870956	7	33	theme	acid	986:989	arg1	pattern					999:1005	ferulic acid release pattern	978:1005	ferulic acid release pattern	978:1005	The formulations were characterized in terms of rheological behavior, biocompatibility, wound healing properties, ferulic acid release pattern and penetration into excised bovine corneas.
31870956	8	34	theme	Pluronic®	1069:1077	arg1	micelles					1079:1086	Pluronic® micelles	1069:1086	Pluronic® micelles that released ferulic acid rapidly	1069:1121	In comparison to Pluronic® micelles that released ferulic acid rapidly, micelle-nanogel composites sustained the release up to 2 days.
31870956	6	35	theme	potential	846:854	arg1	values					856:861	positive zeta potential values	832:861	positive zeta potential values	832:861	Hyaluronan nanogels showed dimensions of ~300 nm with positive zeta potential values.
31870956	2	36	theme	topical	276:282	arg1	administration					284:297	topical administration	276:297	topical administration	276:297	However, ocular bioavailability of efficient antioxidants (e.g. ferulic acid) after topical administration is limited by their poor solubility, low stability and short residence time.
31870956	4	37	theme	F68	649:651	arg1	solutions					662:670	50 and 100 mg/ml Pluronic® F68 micellar solutions	622:670	50 and 100 mg/ml Pluronic® F68 micellar solutions	622:670	Solubility enhancement factor of ferulic acid was found to be equal to 1.9 ± 0.3 and 3.4 ± 0.3 for 50 and 100 mg/ml Pluronic® F68 micellar solutions.
31870956	0	38	theme	ocular	42:47	arg1	delivery					49:56	ferulic acid ocular delivery	29:56	ferulic acid ocular delivery	29:56	Micelle-nanogel platform for ferulic acid ocular delivery.
31870956	4	39	theme	enhancement	534:544	arg1	factor					546:551	Solubility enhancement factor	523:551	Solubility enhancement factor of ferulic acid	523:567	Solubility enhancement factor of ferulic acid was found to be equal to 1.9 ± 0.3 and 3.4 ± 0.3 for 50 and 100 mg/ml Pluronic® F68 micellar solutions.
31870956	6	40	theme	zeta	841:844	arg1	values					856:861	positive zeta potential values	832:861	positive zeta potential values	832:861	Hyaluronan nanogels showed dimensions of ~300 nm with positive zeta potential values.
31870956	3	41	theme	ferulic	414:420	arg1	acid					422:425	ferulic acid	414:425	ferulic acid in a nanocomposite platform composed of nanogels and micelles for efficient delivery to cornea	414:520	The aim of this work was to formulate ferulic acid in a nanocomposite platform composed of nanogels and micelles for efficient delivery to cornea.
31870956	0	42	theme	acid	37:40	arg1	delivery					49:56	ferulic acid ocular delivery	29:56	ferulic acid ocular delivery	29:56	Micelle-nanogel platform for ferulic acid ocular delivery.
31870956	6	43	theme	positive	832:839	arg1	values					856:861	positive zeta potential values	832:861	positive zeta potential values	832:861	Hyaluronan nanogels showed dimensions of ~300 nm with positive zeta potential values.
31870956	8	44	dep	micelles	1079:1086	arg1	comparison					1055:1064	comparison	1055:1064	comparison	1055:1064	In comparison to Pluronic® micelles that released ferulic acid rapidly, micelle-nanogel composites sustained the release up to 2 days.
31870956	7	45	theme	bovine	1036:1041	arg1	corneas					1043:1049	excised bovine corneas	1028:1049	excised bovine corneas	1028:1049	The formulations were characterized in terms of rheological behavior, biocompatibility, wound healing properties, ferulic acid release pattern and penetration into excised bovine corneas.
31870956	8	46	theme	micelle-nanogel	1124:1138	arg1	composites					1140:1149	micelle-nanogel composites	1124:1149	micelle-nanogel composites	1124:1149	In comparison to Pluronic® micelles that released ferulic acid rapidly, micelle-nanogel composites sustained the release up to 2 days.
31870956	2	47	theme	ocular	201:206	arg1	bioavailability					208:222	ocular bioavailability	201:222	ocular bioavailability of efficient antioxidants (e.g. ferulic acid) after topical administration	201:297	However, ocular bioavailability of efficient antioxidants (e.g. ferulic acid) after topical administration is limited by their poor solubility, low stability and short residence time.
31870956	3	48	from	acid	422:425	arg1	platform					446:453	a nanocomposite platform	430:453	a nanocomposite platform composed of nanogels and micelles for efficient delivery to cornea	430:520	The aim of this work was to formulate ferulic acid in a nanocomposite platform composed of nanogels and micelles for efficient delivery to cornea.
31870956	1	49	theme	chemical	101:108	arg1	injury					110:115	a chemical injury	99:115	a chemical injury	99:115	Corneal wound healing after a trauma or a chemical injury has been shown to correlate with antioxidant levels at the ocular surface.
31870956	8	50	theme	ferulic	1102:1108	arg1	acid					1110:1113	ferulic acid	1102:1113	ferulic acid	1102:1113	In comparison to Pluronic® micelles that released ferulic acid rapidly, micelle-nanogel composites sustained the release up to 2 days.
31870956	6	51	theme	~300 nm	819:825	arg1	dimensions					805:814	dimensions	805:814	dimensions of ~300 nm with positive zeta potential values	805:861	Hyaluronan nanogels showed dimensions of ~300 nm with positive zeta potential values.
31870956	7	52	theme	release	991:997	arg1	pattern					999:1005	ferulic acid release pattern	978:1005	ferulic acid release pattern	978:1005	The formulations were characterized in terms of rheological behavior, biocompatibility, wound healing properties, ferulic acid release pattern and penetration into excised bovine corneas.
31870956	2	53	theme	poor	319:322	arg1	solubility					324:333	their poor solubility	313:333	their poor solubility	313:333	However, ocular bioavailability of efficient antioxidants (e.g. ferulic acid) after topical administration is limited by their poor solubility, low stability and short residence time.
31870956	9	54	theme	in	1240:1241	arg1	closure					1255:1261	in vitro wound closure	1240:1261	in vitro wound closure promoting fibroblasts growth and ex vivo accumulation of ferulic acid into both healthy and damaged corneas (>100 µg/cm2)	1240:1383	Furthermore, the micelle-nanogel formulation favored in vitro wound closure promoting fibroblasts growth and ex vivo accumulation of ferulic acid into both healthy and damaged corneas (>100 µg/cm2).
31870956	9	55	theme	micelle-nanogel	1204:1218	arg1	formulation					1220:1230	the micelle-nanogel formulation	1200:1230	the micelle-nanogel formulation	1200:1230	Furthermore, the micelle-nanogel formulation favored in vitro wound closure promoting fibroblasts growth and ex vivo accumulation of ferulic acid into both healthy and damaged corneas (>100 µg/cm2).
31870956	7	56	theme	healing	958:964	arg1	properties					966:975	wound healing properties	952:975	wound healing properties	952:975	The formulations were characterized in terms of rheological behavior, biocompatibility, wound healing properties, ferulic acid release pattern and penetration into excised bovine corneas.
31870956	3	57	theme	nanocomposite	432:444	arg1	platform					446:453	a nanocomposite platform	430:453	a nanocomposite platform composed of nanogels and micelles for efficient delivery to cornea	430:520	The aim of this work was to formulate ferulic acid in a nanocomposite platform composed of nanogels and micelles for efficient delivery to cornea.
31870956	7	58	theme	pattern	999:1005	arg1	terms					903:907	terms	903:907	terms of rheological behavior, biocompatibility, wound healing properties, ferulic acid release pattern and penetration into excised bovine corneas	903:1049	The formulations were characterized in terms of rheological behavior, biocompatibility, wound healing properties, ferulic acid release pattern and penetration into excised bovine corneas.
31870956	7	59	theme	ferulic	978:984	arg1	pattern					999:1005	ferulic acid release pattern	978:1005	ferulic acid release pattern	978:1005	The formulations were characterized in terms of rheological behavior, biocompatibility, wound healing properties, ferulic acid release pattern and penetration into excised bovine corneas.
31870956	5	60	theme	acid	715:718	arg1	micelles					727:734	blank and ferulic acid loaded micelles	697:734	blank and ferulic acid loaded micelles	697:734	Hyaluronan was added to blank and ferulic acid loaded micelles, and then cross-linked with ε-polylysine.
31870956	9	61	theme	ex	1296:1297	arg1	accumulation					1304:1315	ex vivo accumulation	1296:1315	ex vivo accumulation of ferulic acid into both healthy and damaged corneas (>100 µg/cm2)	1296:1383	Furthermore, the micelle-nanogel formulation favored in vitro wound closure promoting fibroblasts growth and ex vivo accumulation of ferulic acid into both healthy and damaged corneas (>100 µg/cm2).
31870956	9	61	theme	ex	1296:1297	arg1	fibroblasts					1273:1283	fibroblasts growth and ex vivo accumulation of ferulic acid into both healthy and damaged corneas (>100 µg/cm2)	1273:1383	fibroblasts growth and ex vivo accumulation of ferulic acid into both healthy and damaged corneas (>100 µg/cm2)	1273:1383	Furthermore, the micelle-nanogel formulation favored in vitro wound closure promoting fibroblasts growth and ex vivo accumulation of ferulic acid into both healthy and damaged corneas (>100 µg/cm2).
31870956	2	62	theme	antioxidants	237:248	arg1	bioavailability					208:222	ocular bioavailability	201:222	ocular bioavailability of efficient antioxidants (e.g. ferulic acid) after topical administration	201:297	However, ocular bioavailability of efficient antioxidants (e.g. ferulic acid) after topical administration is limited by their poor solubility, low stability and short residence time.
31870956	6	63	with	dimensions	805:814	arg1	values					856:861	positive zeta potential values	832:861	positive zeta potential values	832:861	Hyaluronan nanogels showed dimensions of ~300 nm with positive zeta potential values.
31870956	2	64	dep	acid	264:267	arg1	e.g.					251:254	e.g.	251:254	e.g.	251:254	However, ocular bioavailability of efficient antioxidants (e.g. ferulic acid) after topical administration is limited by their poor solubility, low stability and short residence time.
31870956	5	65	theme	loaded	720:725	arg1	micelles					727:734	blank and ferulic acid loaded micelles	697:734	blank and ferulic acid loaded micelles	697:734	Hyaluronan was added to blank and ferulic acid loaded micelles, and then cross-linked with ε-polylysine.
31870956	2	66	theme	efficient	227:235	arg1	antioxidants					237:248	efficient antioxidants	227:248	efficient antioxidants (e.g. ferulic acid)	227:268	However, ocular bioavailability of efficient antioxidants (e.g. ferulic acid) after topical administration is limited by their poor solubility, low stability and short residence time.
31870956	7	67	theme	properties	966:975	arg1	terms					903:907	terms	903:907	terms of rheological behavior, biocompatibility, wound healing properties, ferulic acid release pattern and penetration into excised bovine corneas	903:1049	The formulations were characterized in terms of rheological behavior, biocompatibility, wound healing properties, ferulic acid release pattern and penetration into excised bovine corneas.
30123482	5	0	theme	homeostatic	1238:1248	arg1	pathways					1250:1257	homeostatic pathways	1238:1257	homeostatic pathways	1238:1257	Beyond imaging techniques, this integrative approach provides unprecedented details about the frequency and structure-specific distribution of cell surface sialylation, a critical feature that regulates cellular interactions and homeostatic pathways.
30123482	4	1	theme	glycoforms	935:944	arg1	number					911:916	a higher number	902:916	a higher number of heterogeneous glycoforms	902:944	To further explore the propensity for sialylation, we subsequently mapped the native glycome of model epithelial cell surfaces and illustrate that while glycosylation sites span broadly across the extracellular region, a higher number of heterogeneous glycoforms occur on sialylated sites closest to the transmembrane domain.
30123482	3	2	theme	acids	522:526	arg1	placement					496:504	the placement	492:504	the placement of azido sialic acids on membrane glycoconjugates, which revealed substantial variations in incorporation efficiencies between N-/O-glycans, glycosites, and glycosphingolipids	492:680	Here we employed a mass spectrometric approach to trace the placement of azido sialic acids on membrane glycoconjugates, which revealed substantial variations in incorporation efficiencies between N-/O-glycans, glycosites, and glycosphingolipids.
30123482	5	3	theme	imaging	1016:1022	arg1	techniques					1024:1033	imaging techniques	1016:1033	imaging techniques	1016:1033	Beyond imaging techniques, this integrative approach provides unprecedented details about the frequency and structure-specific distribution of cell surface sialylation, a critical feature that regulates cellular interactions and homeostatic pathways.
30123482	0	4	theme	sialylation	86:96	arg1	heterogeneity					26:38	heterogeneity	26:38	heterogeneity	26:38	Membrane glycomics reveal heterogeneity and quantitative distribution of cell surface sialylation.
30123482	0	4	theme	sialylation	86:96	arg1	distribution					57:68	quantitative distribution	44:68	quantitative distribution	44:68	Membrane glycomics reveal heterogeneity and quantitative distribution of cell surface sialylation.
30123482	4	5	theme	heterogeneous	921:933	arg1	glycoforms					935:944	heterogeneous glycoforms	921:944	heterogeneous glycoforms	921:944	To further explore the propensity for sialylation, we subsequently mapped the native glycome of model epithelial cell surfaces and illustrate that while glycosylation sites span broadly across the extracellular region, a higher number of heterogeneous glycoforms occur on sialylated sites closest to the transmembrane domain.
30123482	1	6	theme	sialylation	277:287	arg1	preferences					262:272	the temporal and localization preferences	232:272	preferences	262:272	Given that unnatural sugar expression is metabolically achieved, the kinetics and disposition of incorporation can lend insight into the temporal and localization preferences of sialylation across the cell surface.
30123482	4	7	theme	surfaces	801:808	arg1	glycome					768:774	the native glycome	757:774	the native glycome of model epithelial cell surfaces	757:808	To further explore the propensity for sialylation, we subsequently mapped the native glycome of model epithelial cell surfaces and illustrate that while glycosylation sites span broadly across the extracellular region, a higher number of heterogeneous glycoforms occur on sialylated sites closest to the transmembrane domain.
30123482	4	8	theme	cell	796:799	arg1	surfaces					801:808	model epithelial cell surfaces	779:808	model epithelial cell surfaces	779:808	To further explore the propensity for sialylation, we subsequently mapped the native glycome of model epithelial cell surfaces and illustrate that while glycosylation sites span broadly across the extracellular region, a higher number of heterogeneous glycoforms occur on sialylated sites closest to the transmembrane domain.
30123482	5	9	theme	integrative	1041:1051	arg1	approach					1053:1060	this integrative approach	1036:1060	this integrative approach	1036:1060	Beyond imaging techniques, this integrative approach provides unprecedented details about the frequency and structure-specific distribution of cell surface sialylation, a critical feature that regulates cellular interactions and homeostatic pathways.
30123482	3	10	theme	substantial	572:582	arg1	variations					584:593	substantial variations	572:593	substantial variations in incorporation efficiencies between N-/O-glycans, glycosites, and glycosphingolipids	572:680	Here we employed a mass spectrometric approach to trace the placement of azido sialic acids on membrane glycoconjugates, which revealed substantial variations in incorporation efficiencies between N-/O-glycans, glycosites, and glycosphingolipids.
30123482	4	11	theme	higher	904:909	arg1	number					911:916	a higher number	902:916	a higher number of heterogeneous glycoforms	902:944	To further explore the propensity for sialylation, we subsequently mapped the native glycome of model epithelial cell surfaces and illustrate that while glycosylation sites span broadly across the extracellular region, a higher number of heterogeneous glycoforms occur on sialylated sites closest to the transmembrane domain.
30123482	2	12	dep	diversity	389:397	arg1	the					375:377	the	375:377	the	375:377	However, common detection schemes lack the ability to detail the molecular diversity and distribution of target moieties.
30123482	5	13	dep	frequency	1103:1111	arg1	the					1099:1101	the	1099:1101	the	1099:1101	Beyond imaging techniques, this integrative approach provides unprecedented details about the frequency and structure-specific distribution of cell surface sialylation, a critical feature that regulates cellular interactions and homeostatic pathways.
30123482	2	14	theme	target	419:424	arg1	moieties					426:433	target moieties	419:433	target moieties	419:433	However, common detection schemes lack the ability to detail the molecular diversity and distribution of target moieties.
30123482	5	15	theme	cellular	1212:1219	arg1	interactions					1221:1232	cellular interactions	1212:1232	cellular interactions	1212:1232	Beyond imaging techniques, this integrative approach provides unprecedented details about the frequency and structure-specific distribution of cell surface sialylation, a critical feature that regulates cellular interactions and homeostatic pathways.
30123482	3	16	from	placement	496:504	arg1	glycoconjugates					540:554	membrane glycoconjugates	531:554	membrane glycoconjugates	531:554	Here we employed a mass spectrometric approach to trace the placement of azido sialic acids on membrane glycoconjugates, which revealed substantial variations in incorporation efficiencies between N-/O-glycans, glycosites, and glycosphingolipids.
30123482	3	17	theme	spectrometric	460:472	arg1	approach					474:481	a mass spectrometric approach	453:481	a mass spectrometric approach	453:481	Here we employed a mass spectrometric approach to trace the placement of azido sialic acids on membrane glycoconjugates, which revealed substantial variations in incorporation efficiencies between N-/O-glycans, glycosites, and glycosphingolipids.
30123482	4	18	gly	glycosylation	836:848	arg2	sites					850:854	glycosylation sites	836:854	glycosylation sites	836:854	To further explore the propensity for sialylation, we subsequently mapped the native glycome of model epithelial cell surfaces and illustrate that while glycosylation sites span broadly across the extracellular region, a higher number of heterogeneous glycoforms occur on sialylated sites closest to the transmembrane domain.
30123482	3	19	theme	incorporation	598:610	arg1	efficiencies					612:623	incorporation efficiencies	598:623	incorporation efficiencies	598:623	Here we employed a mass spectrometric approach to trace the placement of azido sialic acids on membrane glycoconjugates, which revealed substantial variations in incorporation efficiencies between N-/O-glycans, glycosites, and glycosphingolipids.
30123482	1	20	theme	incorporation	196:208	arg1	disposition					181:191	disposition	181:191	disposition	181:191	Given that unnatural sugar expression is metabolically achieved, the kinetics and disposition of incorporation can lend insight into the temporal and localization preferences of sialylation across the cell surface.
30123482	1	20	theme	incorporation	196:208	arg1	kinetics					168:175	kinetics	168:175	kinetics	168:175	Given that unnatural sugar expression is metabolically achieved, the kinetics and disposition of incorporation can lend insight into the temporal and localization preferences of sialylation across the cell surface.
30123482	0	21	theme	Membrane	0:7	arg1	glycomics					9:17	Membrane glycomics	0:17	Membrane glycomics	0:17	Membrane glycomics reveal heterogeneity and quantitative distribution of cell surface sialylation.
30123482	1	22	theme	cell	300:303	arg1	surface					305:311	the cell surface	296:311	the cell surface	296:311	Given that unnatural sugar expression is metabolically achieved, the kinetics and disposition of incorporation can lend insight into the temporal and localization preferences of sialylation across the cell surface.
30123482	2	23	theme	detection	330:338	arg1	schemes					340:346	common detection schemes	323:346	common detection schemes	323:346	However, common detection schemes lack the ability to detail the molecular diversity and distribution of target moieties.
30123482	4	24	theme	transmembrane	987:999	arg1	domain					1001:1006	the transmembrane domain	983:1006	the transmembrane domain	983:1006	To further explore the propensity for sialylation, we subsequently mapped the native glycome of model epithelial cell surfaces and illustrate that while glycosylation sites span broadly across the extracellular region, a higher number of heterogeneous glycoforms occur on sialylated sites closest to the transmembrane domain.
30123482	2	25	theme	common	323:328	arg1	schemes					340:346	common detection schemes	323:346	common detection schemes	323:346	However, common detection schemes lack the ability to detail the molecular diversity and distribution of target moieties.
30123482	3	26	gly	glycosites	647:656	arg2	glycosites					647:656	glycosites	647:656	glycosites	647:656	Here we employed a mass spectrometric approach to trace the placement of azido sialic acids on membrane glycoconjugates, which revealed substantial variations in incorporation efficiencies between N-/O-glycans, glycosites, and glycosphingolipids.
30123482	4	27	theme	epithelial	785:794	arg1	surfaces					801:808	model epithelial cell surfaces	779:808	model epithelial cell surfaces	779:808	To further explore the propensity for sialylation, we subsequently mapped the native glycome of model epithelial cell surfaces and illustrate that while glycosylation sites span broadly across the extracellular region, a higher number of heterogeneous glycoforms occur on sialylated sites closest to the transmembrane domain.
30123482	4	28	theme	glycosylation	836:848	arg1	sites					850:854	glycosylation sites	836:854	glycosylation sites	836:854	To further explore the propensity for sialylation, we subsequently mapped the native glycome of model epithelial cell surfaces and illustrate that while glycosylation sites span broadly across the extracellular region, a higher number of heterogeneous glycoforms occur on sialylated sites closest to the transmembrane domain.
30123482	1	29	theme	sugar	120:124	arg1	expression					126:135	unnatural sugar expression	110:135	unnatural sugar expression	110:135	Given that unnatural sugar expression is metabolically achieved, the kinetics and disposition of incorporation can lend insight into the temporal and localization preferences of sialylation across the cell surface.
30123482	4	30	gly	sialylated	955:964	arg1	sites					966:970	sialylated sites	955:970	sialylated sites closest to the transmembrane domain	955:1006	To further explore the propensity for sialylation, we subsequently mapped the native glycome of model epithelial cell surfaces and illustrate that while glycosylation sites span broadly across the extracellular region, a higher number of heterogeneous glycoforms occur on sialylated sites closest to the transmembrane domain.
30123482	0	31	theme	quantitative	44:55	arg1	distribution					57:68	quantitative distribution	44:68	quantitative distribution	44:68	Membrane glycomics reveal heterogeneity and quantitative distribution of cell surface sialylation.
30123482	4	32	theme	native	761:766	arg1	glycome					768:774	the native glycome	757:774	the native glycome of model epithelial cell surfaces	757:808	To further explore the propensity for sialylation, we subsequently mapped the native glycome of model epithelial cell surfaces and illustrate that while glycosylation sites span broadly across the extracellular region, a higher number of heterogeneous glycoforms occur on sialylated sites closest to the transmembrane domain.
30123482	4	33	theme	extracellular	880:892	arg1	region					894:899	the extracellular region	876:899	the extracellular region	876:899	To further explore the propensity for sialylation, we subsequently mapped the native glycome of model epithelial cell surfaces and illustrate that while glycosylation sites span broadly across the extracellular region, a higher number of heterogeneous glycoforms occur on sialylated sites closest to the transmembrane domain.
30123482	3	34	theme	mass	455:458	arg1	approach					474:481	a mass spectrometric approach	453:481	a mass spectrometric approach	453:481	Here we employed a mass spectrometric approach to trace the placement of azido sialic acids on membrane glycoconjugates, which revealed substantial variations in incorporation efficiencies between N-/O-glycans, glycosites, and glycosphingolipids.
30123482	5	35	theme	critical	1180:1187	arg1	sialylation					1165:1175	cell surface sialylation	1152:1175	cell surface sialylation	1152:1175	Beyond imaging techniques, this integrative approach provides unprecedented details about the frequency and structure-specific distribution of cell surface sialylation, a critical feature that regulates cellular interactions and homeostatic pathways.
30123482	5	35	theme	critical	1180:1187	arg1	feature					1189:1195	a critical feature	1178:1195	a critical feature that regulates cellular interactions and homeostatic pathways	1178:1257	Beyond imaging techniques, this integrative approach provides unprecedented details about the frequency and structure-specific distribution of cell surface sialylation, a critical feature that regulates cellular interactions and homeostatic pathways.
30123482	2	36	theme	moieties	426:433	arg1	distribution					403:414	distribution	403:414	distribution	403:414	However, common detection schemes lack the ability to detail the molecular diversity and distribution of target moieties.
30123482	2	36	theme	moieties	426:433	arg1	diversity					389:397	diversity	389:397	diversity	389:397	However, common detection schemes lack the ability to detail the molecular diversity and distribution of target moieties.
30123482	5	37	theme	structure-specific	1117:1134	arg1	distribution					1136:1147	structure-specific distribution	1117:1147	structure-specific distribution	1117:1147	Beyond imaging techniques, this integrative approach provides unprecedented details about the frequency and structure-specific distribution of cell surface sialylation, a critical feature that regulates cellular interactions and homeostatic pathways.
30123482	3	38	theme	azido	509:513	arg1	acids					522:526	azido sialic acids	509:526	azido sialic acids	509:526	Here we employed a mass spectrometric approach to trace the placement of azido sialic acids on membrane glycoconjugates, which revealed substantial variations in incorporation efficiencies between N-/O-glycans, glycosites, and glycosphingolipids.
30123482	4	39	theme	sialylated	955:964	arg1	sites					966:970	sialylated sites	955:970	sialylated sites closest to the transmembrane domain	955:1006	To further explore the propensity for sialylation, we subsequently mapped the native glycome of model epithelial cell surfaces and illustrate that while glycosylation sites span broadly across the extracellular region, a higher number of heterogeneous glycoforms occur on sialylated sites closest to the transmembrane domain.
30123482	0	40	gly	heterogeneity	26:38	arg1	sialylation					86:96	cell surface sialylation	73:96	cell surface sialylation	73:96	Membrane glycomics reveal heterogeneity and quantitative distribution of cell surface sialylation.
30123482	3	41	theme	sialic	515:520	arg1	acids					522:526	azido sialic acids	509:526	azido sialic acids	509:526	Here we employed a mass spectrometric approach to trace the placement of azido sialic acids on membrane glycoconjugates, which revealed substantial variations in incorporation efficiencies between N-/O-glycans, glycosites, and glycosphingolipids.
30123482	3	42	from	variations	584:593	arg1	efficiencies					612:623	incorporation efficiencies	598:623	incorporation efficiencies	598:623	Here we employed a mass spectrometric approach to trace the placement of azido sialic acids on membrane glycoconjugates, which revealed substantial variations in incorporation efficiencies between N-/O-glycans, glycosites, and glycosphingolipids.
30123482	5	43	theme	cell	1152:1155	arg1	sialylation					1165:1175	cell surface sialylation	1152:1175	cell surface sialylation	1152:1175	Beyond imaging techniques, this integrative approach provides unprecedented details about the frequency and structure-specific distribution of cell surface sialylation, a critical feature that regulates cellular interactions and homeostatic pathways.
30123482	5	43	theme	cell	1152:1155	arg1	feature					1189:1195	a critical feature	1178:1195	a critical feature that regulates cellular interactions and homeostatic pathways	1178:1257	Beyond imaging techniques, this integrative approach provides unprecedented details about the frequency and structure-specific distribution of cell surface sialylation, a critical feature that regulates cellular interactions and homeostatic pathways.
30123482	5	44	theme	unprecedented	1071:1083	arg1	details					1085:1091	unprecedented details	1071:1091	unprecedented details about the frequency and structure-specific distribution of cell surface sialylation, a critical feature that regulates cellular interactions and homeostatic pathways	1071:1257	Beyond imaging techniques, this integrative approach provides unprecedented details about the frequency and structure-specific distribution of cell surface sialylation, a critical feature that regulates cellular interactions and homeostatic pathways.
30123482	4	45	theme	model	779:783	arg1	surfaces					801:808	model epithelial cell surfaces	779:808	model epithelial cell surfaces	779:808	To further explore the propensity for sialylation, we subsequently mapped the native glycome of model epithelial cell surfaces and illustrate that while glycosylation sites span broadly across the extracellular region, a higher number of heterogeneous glycoforms occur on sialylated sites closest to the transmembrane domain.
30123482	1	46	theme	unnatural	110:118	arg1	expression					126:135	unnatural sugar expression	110:135	unnatural sugar expression	110:135	Given that unnatural sugar expression is metabolically achieved, the kinetics and disposition of incorporation can lend insight into the temporal and localization preferences of sialylation across the cell surface.
30123482	1	47	theme	temporal	236:243	arg1	preferences					262:272	the temporal and localization preferences	232:272	preferences	262:272	Given that unnatural sugar expression is metabolically achieved, the kinetics and disposition of incorporation can lend insight into the temporal and localization preferences of sialylation across the cell surface.
30123482	4	48	theme	closest	972:978	arg1	sites					966:970	sialylated sites	955:970	sialylated sites closest to the transmembrane domain	955:1006	To further explore the propensity for sialylation, we subsequently mapped the native glycome of model epithelial cell surfaces and illustrate that while glycosylation sites span broadly across the extracellular region, a higher number of heterogeneous glycoforms occur on sialylated sites closest to the transmembrane domain.
30123482	5	49	theme	surface	1157:1163	arg1	sialylation					1165:1175	cell surface sialylation	1152:1175	cell surface sialylation	1152:1175	Beyond imaging techniques, this integrative approach provides unprecedented details about the frequency and structure-specific distribution of cell surface sialylation, a critical feature that regulates cellular interactions and homeostatic pathways.
30123482	5	49	theme	surface	1157:1163	arg1	feature					1189:1195	a critical feature	1178:1195	a critical feature that regulates cellular interactions and homeostatic pathways	1178:1257	Beyond imaging techniques, this integrative approach provides unprecedented details about the frequency and structure-specific distribution of cell surface sialylation, a critical feature that regulates cellular interactions and homeostatic pathways.
30123482	0	50	theme	surface	78:84	arg1	sialylation					86:96	cell surface sialylation	73:96	cell surface sialylation	73:96	Membrane glycomics reveal heterogeneity and quantitative distribution of cell surface sialylation.
30123482	5	51	theme	sialylation	1165:1175	arg1	frequency					1103:1111	frequency	1103:1111	frequency	1103:1111	Beyond imaging techniques, this integrative approach provides unprecedented details about the frequency and structure-specific distribution of cell surface sialylation, a critical feature that regulates cellular interactions and homeostatic pathways.
30123482	5	51	theme	sialylation	1165:1175	arg1	distribution					1136:1147	structure-specific distribution	1117:1147	structure-specific distribution	1117:1147	Beyond imaging techniques, this integrative approach provides unprecedented details about the frequency and structure-specific distribution of cell surface sialylation, a critical feature that regulates cellular interactions and homeostatic pathways.
30123482	3	52	theme	membrane	531:538	arg1	glycoconjugates					540:554	membrane glycoconjugates	531:554	membrane glycoconjugates	531:554	Here we employed a mass spectrometric approach to trace the placement of azido sialic acids on membrane glycoconjugates, which revealed substantial variations in incorporation efficiencies between N-/O-glycans, glycosites, and glycosphingolipids.
30123482	1	53	theme	localization	249:260	arg1	preferences					262:272	the temporal and localization preferences	232:272	preferences	262:272	Given that unnatural sugar expression is metabolically achieved, the kinetics and disposition of incorporation can lend insight into the temporal and localization preferences of sialylation across the cell surface.
30123482	0	54	theme	cell	73:76	arg1	sialylation					86:96	cell surface sialylation	73:96	cell surface sialylation	73:96	Membrane glycomics reveal heterogeneity and quantitative distribution of cell surface sialylation.
30123482	2	55	theme	molecular	379:387	arg1	diversity					389:397	diversity	389:397	diversity	389:397	However, common detection schemes lack the ability to detail the molecular diversity and distribution of target moieties.
31722994	6	0	theme	known	944:948	arg1	proteins					963:970	the known glycosylated proteins	940:970	the known glycosylated proteins	940:970	Surprisingly, the abundance of most of the known glycosylated proteins did not significantly change in the glycosylation-defective mutants, except for BCAL1086 and BCAL2974, which were found in reduced amounts, suggesting they could be degraded.
31722994	10	1	dep	pathogen	1929:1936	arg1	cenocepacia					1951:1961	the opportunistic pathogen Burkholderia cenocepacia	1911:1961	the opportunistic pathogen Burkholderia cenocepacia	1911:1961	In this work, we investigated the effect of the disruption of O-linked glycosylation in the opportunistic pathogen Burkholderia cenocepacia using a combination of proteomic, molecular, and phenotypic assays.
31722994	2	2	from	proteins	329:336	arg1	cenocepacia					354:364	Burkholderia cenocepacia	341:364	Burkholderia cenocepacia	341:364	The addition of the trisaccharide β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc to membrane exported proteins in Burkholderia cenocepacia is required for bacterial fitness and resistance to environmental stress.
31722994	8	3	theme	cell	1382:1385	arg1	reprogramming					1387:1399	a global cell reprogramming	1373:1399	a global cell reprogramming	1373:1399	Together, our results show that loss of glycosylation in B. cenocepacia results in a global cell reprogramming via alteration of the transcriptional regulatory systems, which cannot be explained by the abundance changes in known B. cenocepacia glycoproteins.IMPORTANCE Protein glycosylation is increasingly recognized as a common posttranslational protein modification in bacterial species.
31722994	2	4	theme	1,3	294:296	arg1	-β-GalNAc					298:306	the trisaccharide β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc	254:306	the trisaccharide β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc	254:306	The addition of the trisaccharide β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc to membrane exported proteins in Burkholderia cenocepacia is required for bacterial fitness and resistance to environmental stress.
31722994	11	5	theme	most	2221:2224	arg1	glycoproteins					2226:2238	most glycoproteins	2221:2238	most glycoproteins	2221:2238	We find that in contrast to recent findings on the N-linked glycosylation systems of Campylobacter jejuni, O-linked glycosylation does not appear to play a role in proteome stabilization of most glycoproteins.
31722994	4	6	theme	transcriptional	670:684	arg1	regulation					686:695	transcriptional regulation	670:695	transcriptional regulation of multiple proteins, including the repression of the master quorum CepR/I	670:770	Using proteomics, luciferase reporter assays, and DNA cross-linking, we demonstrate the loss of glycosylation leads to changes in transcriptional regulation of multiple proteins, including the repression of the master quorum CepR/I.
31722994	10	7	theme	phenotypic	2012:2021	arg1	assays					2023:2028	proteomic, molecular, and phenotypic assays	1986:2028	proteomic, molecular, and phenotypic assays	1986:2028	In this work, we investigated the effect of the disruption of O-linked glycosylation in the opportunistic pathogen Burkholderia cenocepacia using a combination of proteomic, molecular, and phenotypic assays.
31722994	8	8	from	modification	1646:1657	arg1	species					1672:1678	bacterial species	1662:1678	bacterial species	1662:1678	Together, our results show that loss of glycosylation in B. cenocepacia results in a global cell reprogramming via alteration of the transcriptional regulatory systems, which cannot be explained by the abundance changes in known B. cenocepacia glycoproteins.IMPORTANCE Protein glycosylation is increasingly recognized as a common posttranslational protein modification in bacterial species.
31722994	2	9	theme	-α-GalNAc-	283:292	arg1	-β-GalNAc					298:306	the trisaccharide β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc	254:306	the trisaccharide β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc	254:306	The addition of the trisaccharide β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc to membrane exported proteins in Burkholderia cenocepacia is required for bacterial fitness and resistance to environmental stress.
31722994	9	10	theme	glycosylation	1745:1757	arg1	systems					1759:1765	most glycosylation systems	1740:1765	most glycosylation systems	1740:1765	Despite this commonality, our understanding of the role of most glycosylation systems in bacterial physiology and pathogenesis is incomplete.
31722994	11	11	from	findings	2066:2073	arg1	systems					2105:2111	the N-linked glycosylation systems	2078:2111	the N-linked glycosylation systems of Campylobacter jejuni	2078:2135	We find that in contrast to recent findings on the N-linked glycosylation systems of Campylobacter jejuni, O-linked glycosylation does not appear to play a role in proteome stabilization of most glycoproteins.
31722994	10	12	theme	O-linked	1885:1892	arg1	glycosylation					1894:1906	O-linked glycosylation	1885:1906	O-linked glycosylation	1885:1906	In this work, we investigated the effect of the disruption of O-linked glycosylation in the opportunistic pathogen Burkholderia cenocepacia using a combination of proteomic, molecular, and phenotypic assays.
31722994	2	13	theme	membrane	311:318	arg1	proteins					329:336	membrane exported proteins	311:336	membrane exported proteins in Burkholderia cenocepacia	311:364	The addition of the trisaccharide β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc to membrane exported proteins in Burkholderia cenocepacia is required for bacterial fitness and resistance to environmental stress.
31722994	4	14	from	changes	659:665	arg1	regulation					686:695	transcriptional regulation	670:695	transcriptional regulation of multiple proteins, including the repression of the master quorum CepR/I	670:770	Using proteomics, luciferase reporter assays, and DNA cross-linking, we demonstrate the loss of glycosylation leads to changes in transcriptional regulation of multiple proteins, including the repression of the master quorum CepR/I.
31722994	14	15	theme	glycosylation	2747:2759	arg1	effects					2727:2733	the pleiotropic effects	2711:2733	the pleiotropic effects of O-linked glycosylation in B. cenocepacia	2711:2777	This research unravels the pleiotropic effects of O-linked glycosylation in B. cenocepacia, demonstrating that its loss does not simply affect the stability of the glycoproteome, but also interferes with transcription and the broader proteome.
31722994	8	16	theme	global	1375:1380	arg1	reprogramming					1387:1399	a global cell reprogramming	1373:1399	a global cell reprogramming	1373:1399	Together, our results show that loss of glycosylation in B. cenocepacia results in a global cell reprogramming via alteration of the transcriptional regulatory systems, which cannot be explained by the abundance changes in known B. cenocepacia glycoproteins.IMPORTANCE Protein glycosylation is increasingly recognized as a common posttranslational protein modification in bacterial species.
31722994	0	17	theme	Transcriptional	129:143	arg1	Regulators					145:154	Transcriptional Regulators	129:154	Transcriptional Regulators	129:154	Loss of O-Linked Protein Glycosylation in Burkholderia cenocepacia Impairs Biofilm Formation and Siderophore Activity and Alters Transcriptional Regulators.
31722994	2	18	theme	-β-GalNAc	298:306	arg1	addition					242:249	The addition	238:249	The addition of the trisaccharide β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc to membrane exported proteins in Burkholderia cenocepacia	238:364	The addition of the trisaccharide β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc to membrane exported proteins in Burkholderia cenocepacia is required for bacterial fitness and resistance to environmental stress.
31722994	1	19	theme	genus	231:235	arg1	glycosylation					174:186	O-linked protein glycosylation	157:186	O-linked protein glycosylation	157:186	O-linked protein glycosylation is a conserved feature of the Burkholderia genus.
31722994	1	19	theme	genus	231:235	arg1	feature					203:209	a conserved feature	191:209	a conserved feature of the Burkholderia genus	191:235	O-linked protein glycosylation is a conserved feature of the Burkholderia genus.
31722994	5	20	theme	biofilm	846:852	arg1	formation					854:862	biofilm formation	846:862	biofilm formation	846:862	These proteomic and transcriptional alterations lead to the abolition of biofilm formation and defects in siderophore activity.
31722994	5	21	theme	siderophore	879:889	arg1	activity					891:898	siderophore activity	879:898	siderophore activity	879:898	These proteomic and transcriptional alterations lead to the abolition of biofilm formation and defects in siderophore activity.
31722994	9	22	from	role	1732:1735	arg1	physiology					1780:1789	bacterial physiology	1770:1789	bacterial physiology	1770:1789	Despite this commonality, our understanding of the role of most glycosylation systems in bacterial physiology and pathogenesis is incomplete.
31722994	9	22	from	role	1732:1735	arg1	pathogenesis					1795:1806	pathogenesis	1795:1806	pathogenesis	1795:1806	Despite this commonality, our understanding of the role of most glycosylation systems in bacterial physiology and pathogenesis is incomplete.
31722994	13	23	theme	global	2560:2565	arg1	alterations					2597:2607	both global proteomic and transcriptional alterations	2555:2607	both global proteomic and transcriptional alterations	2555:2607	These alterations lead to dramatic phenotypic changes in glycosylation-null strains, which are paralleled by both global proteomic and transcriptional alterations, which do not appear to directly result from the loss of glycosylation per se.
31722994	7	24	theme	proteomic	1223:1231	arg1	alterations					1233:1243	the proteomic alterations	1219:1243	the proteomic alterations	1219:1243	However, the loss of these two proteins was not responsible for driving the proteomic alterations, biofilm formation, or siderophore activity.
31722994	2	25	theme	1,3	279:281	arg1	-β-GalNAc					298:306	the trisaccharide β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc	254:306	the trisaccharide β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc	254:306	The addition of the trisaccharide β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc to membrane exported proteins in Burkholderia cenocepacia is required for bacterial fitness and resistance to environmental stress.
31722994	1	26	theme	O-linked	157:164	arg1	glycosylation					174:186	O-linked protein glycosylation	157:186	O-linked protein glycosylation	157:186	O-linked protein glycosylation is a conserved feature of the Burkholderia genus.
31722994	1	26	theme	O-linked	157:164	arg1	feature					203:209	a conserved feature	191:209	a conserved feature of the Burkholderia genus	191:235	O-linked protein glycosylation is a conserved feature of the Burkholderia genus.
31722994	12	27	theme	regulator	2414:2422	arg1	gene					2440:2443	the quorum-sensing regulator cepR (BCAM1868) gene	2395:2443	the quorum-sensing regulator cepR (BCAM1868) gene	2395:2443	Our results reveal that loss of glycosylation in B. cenocepacia strains leads to global proteome and transcriptional changes, including the repression of the quorum-sensing regulator cepR (BCAM1868) gene.
31722994	4	28	theme	proteins	709:716	arg1	regulation					686:695	transcriptional regulation	670:695	transcriptional regulation of multiple proteins, including the repression of the master quorum CepR/I	670:770	Using proteomics, luciferase reporter assays, and DNA cross-linking, we demonstrate the loss of glycosylation leads to changes in transcriptional regulation of multiple proteins, including the repression of the master quorum CepR/I.
31722994	5	29	theme	proteomic	779:787	arg1	alterations					809:819	These proteomic and transcriptional alterations	773:819	These proteomic and transcriptional alterations	773:819	These proteomic and transcriptional alterations lead to the abolition of biofilm formation and defects in siderophore activity.
31722994	5	30	theme	formation	854:862	arg1	defects					868:874	defects	868:874	defects in siderophore activity	868:898	These proteomic and transcriptional alterations lead to the abolition of biofilm formation and defects in siderophore activity.
31722994	5	30	theme	formation	854:862	arg1	abolition					833:841	the abolition	829:841	the abolition of biofilm formation	829:862	These proteomic and transcriptional alterations lead to the abolition of biofilm formation and defects in siderophore activity.
31722994	8	31	theme	known	1513:1517	arg1	glycosylation					1567:1579	known B. cenocepacia glycoproteins.IMPORTANCE Protein glycosylation	1513:1579	known B. cenocepacia glycoproteins.IMPORTANCE Protein glycosylation is increasingly recognized as a common posttranslational protein modification in bacterial species	1513:1678	Together, our results show that loss of glycosylation in B. cenocepacia results in a global cell reprogramming via alteration of the transcriptional regulatory systems, which cannot be explained by the abundance changes in known B. cenocepacia glycoproteins.IMPORTANCE Protein glycosylation is increasingly recognized as a common posttranslational protein modification in bacterial species.
31722994	14	32	theme	pleiotropic	2715:2725	arg1	effects					2727:2733	the pleiotropic effects	2711:2733	the pleiotropic effects of O-linked glycosylation in B. cenocepacia	2711:2777	This research unravels the pleiotropic effects of O-linked glycosylation in B. cenocepacia, demonstrating that its loss does not simply affect the stability of the glycoproteome, but also interferes with transcription and the broader proteome.
31722994	6	33	gly	glycosylated	950:961	arg1	proteins					963:970	the known glycosylated proteins	940:970	the known glycosylated proteins	940:970	Surprisingly, the abundance of most of the known glycosylated proteins did not significantly change in the glycosylation-defective mutants, except for BCAL1086 and BCAL2974, which were found in reduced amounts, suggesting they could be degraded.
31722994	5	34	theme	transcriptional	793:807	arg1	alterations					809:819	These proteomic and transcriptional alterations	773:819	These proteomic and transcriptional alterations	773:819	These proteomic and transcriptional alterations lead to the abolition of biofilm formation and defects in siderophore activity.
31722994	8	35	from	loss	1322:1325	arg1	cenocepacia					1350:1360	B. cenocepacia	1347:1360	B. cenocepacia	1347:1360	Together, our results show that loss of glycosylation in B. cenocepacia results in a global cell reprogramming via alteration of the transcriptional regulatory systems, which cannot be explained by the abundance changes in known B. cenocepacia glycoproteins.IMPORTANCE Protein glycosylation is increasingly recognized as a common posttranslational protein modification in bacterial species.
31722994	10	36	from	effect	1857:1862	arg1	pathogen					1929:1936	the opportunistic pathogen Burkholderia cenocepacia	1911:1961	the opportunistic pathogen Burkholderia cenocepacia	1911:1961	In this work, we investigated the effect of the disruption of O-linked glycosylation in the opportunistic pathogen Burkholderia cenocepacia using a combination of proteomic, molecular, and phenotypic assays.
31722994	0	37	from	Loss	0:3	arg1	cenocepacia					55:65	Burkholderia cenocepacia	42:65	Burkholderia cenocepacia	42:65	Loss of O-Linked Protein Glycosylation in Burkholderia cenocepacia Impairs Biofilm Formation and Siderophore Activity and Alters Transcriptional Regulators.
31722994	12	38	theme	cenocepacia	2293:2303	arg1	strains					2305:2311	B. cenocepacia strains	2290:2311	B. cenocepacia strains	2290:2311	Our results reveal that loss of glycosylation in B. cenocepacia strains leads to global proteome and transcriptional changes, including the repression of the quorum-sensing regulator cepR (BCAM1868) gene.
31722994	0	39	theme	Siderophore	97:107	arg1	Activity					109:116	Siderophore Activity	97:116	Siderophore Activity	97:116	Loss of O-Linked Protein Glycosylation in Burkholderia cenocepacia Impairs Biofilm Formation and Siderophore Activity and Alters Transcriptional Regulators.
31722994	11	40	theme	glycosylation	2091:2103	arg1	systems					2105:2111	the N-linked glycosylation systems	2078:2111	the N-linked glycosylation systems of Campylobacter jejuni	2078:2135	We find that in contrast to recent findings on the N-linked glycosylation systems of Campylobacter jejuni, O-linked glycosylation does not appear to play a role in proteome stabilization of most glycoproteins.
31722994	11	41	gly	glycoproteins	2226:2238	arg1	glycoproteins					2226:2238	most glycoproteins	2221:2238	most glycoproteins	2221:2238	We find that in contrast to recent findings on the N-linked glycosylation systems of Campylobacter jejuni, O-linked glycosylation does not appear to play a role in proteome stabilization of most glycoproteins.
31722994	11	42	link	N-linked	2082:2089	arg1	systems					2105:2111	the N-linked glycosylation systems	2078:2111	the N-linked glycosylation systems of Campylobacter jejuni	2078:2135	We find that in contrast to recent findings on the N-linked glycosylation systems of Campylobacter jejuni, O-linked glycosylation does not appear to play a role in proteome stabilization of most glycoproteins.
31722994	8	43	theme	Protein	1559:1565	arg1	glycosylation					1567:1579	known B. cenocepacia glycoproteins.IMPORTANCE Protein glycosylation	1513:1579	known B. cenocepacia glycoproteins.IMPORTANCE Protein glycosylation is increasingly recognized as a common posttranslational protein modification in bacterial species	1513:1678	Together, our results show that loss of glycosylation in B. cenocepacia results in a global cell reprogramming via alteration of the transcriptional regulatory systems, which cannot be explained by the abundance changes in known B. cenocepacia glycoproteins.IMPORTANCE Protein glycosylation is increasingly recognized as a common posttranslational protein modification in bacterial species.
31722994	12	44	theme	proteome	2329:2336	arg1	changes					2358:2364	global proteome and transcriptional changes	2322:2364	global proteome and transcriptional changes	2322:2364	Our results reveal that loss of glycosylation in B. cenocepacia strains leads to global proteome and transcriptional changes, including the repression of the quorum-sensing regulator cepR (BCAM1868) gene.
31722994	12	44	theme	proteome	2329:2336	arg1	repression					2381:2390	the repression	2377:2390	the repression of the quorum-sensing regulator cepR (BCAM1868) gene	2377:2443	Our results reveal that loss of glycosylation in B. cenocepacia strains leads to global proteome and transcriptional changes, including the repression of the quorum-sensing regulator cepR (BCAM1868) gene.
31722994	3	45	theme	underlying	453:462	arg1	causes					464:469	the underlying causes	449:469	the underlying causes of the defects observed in the absence of glycosylation	449:525	However, the underlying causes of the defects observed in the absence of glycosylation are unclear.
31722994	3	45	theme	underlying	453:462	arg1	unclear					531:537	unclear	531:537	unclear	531:537	However, the underlying causes of the defects observed in the absence of glycosylation are unclear.
31722994	5	46	from	abolition	833:841	arg1	activity					891:898	siderophore activity	879:898	siderophore activity	879:898	These proteomic and transcriptional alterations lead to the abolition of biofilm formation and defects in siderophore activity.
31722994	0	47	theme	Biofilm	75:81	arg1	Formation					83:91	Biofilm Formation	75:91	Biofilm Formation	75:91	Loss of O-Linked Protein Glycosylation in Burkholderia cenocepacia Impairs Biofilm Formation and Siderophore Activity and Alters Transcriptional Regulators.
31722994	4	48	theme	quorum	758:763	arg1	CepR/I					765:770	the master quorum CepR/I	747:770	the master quorum CepR/I	747:770	Using proteomics, luciferase reporter assays, and DNA cross-linking, we demonstrate the loss of glycosylation leads to changes in transcriptional regulation of multiple proteins, including the repression of the master quorum CepR/I.
31722994	13	49	theme	glycosylation-null	2503:2520	arg1	strains					2522:2528	glycosylation-null strains	2503:2528	glycosylation-null strains	2503:2528	These alterations lead to dramatic phenotypic changes in glycosylation-null strains, which are paralleled by both global proteomic and transcriptional alterations, which do not appear to directly result from the loss of glycosylation per se.
31722994	6	50	theme	reduced	1095:1101	arg1	amounts					1103:1109	reduced amounts	1095:1109	reduced amounts	1095:1109	Surprisingly, the abundance of most of the known glycosylated proteins did not significantly change in the glycosylation-defective mutants, except for BCAL1086 and BCAL2974, which were found in reduced amounts, suggesting they could be degraded.
31722994	4	51	theme	reporter	569:576	arg1	assays					578:583	luciferase reporter assays	558:583	luciferase reporter assays	558:583	Using proteomics, luciferase reporter assays, and DNA cross-linking, we demonstrate the loss of glycosylation leads to changes in transcriptional regulation of multiple proteins, including the repression of the master quorum CepR/I.
31722994	13	52	from	changes	2492:2498	arg1	strains					2522:2528	glycosylation-null strains	2503:2528	glycosylation-null strains	2503:2528	These alterations lead to dramatic phenotypic changes in glycosylation-null strains, which are paralleled by both global proteomic and transcriptional alterations, which do not appear to directly result from the loss of glycosylation per se.
31722994	3	53	theme	defects	478:484	arg1	causes					464:469	the underlying causes	449:469	the underlying causes of the defects observed in the absence of glycosylation	449:525	However, the underlying causes of the defects observed in the absence of glycosylation are unclear.
31722994	3	53	theme	defects	478:484	arg1	unclear					531:537	unclear	531:537	unclear	531:537	However, the underlying causes of the defects observed in the absence of glycosylation are unclear.
31722994	0	54	theme	O-Linked	8:15	arg1	Glycosylation					25:37	O-Linked Protein Glycosylation	8:37	O-Linked Protein Glycosylation	8:37	Loss of O-Linked Protein Glycosylation in Burkholderia cenocepacia Impairs Biofilm Formation and Siderophore Activity and Alters Transcriptional Regulators.
31722994	8	55	theme	glycosylation	1330:1342	arg1	loss					1322:1325	loss	1322:1325	loss of glycosylation in B. cenocepacia	1322:1360	Together, our results show that loss of glycosylation in B. cenocepacia results in a global cell reprogramming via alteration of the transcriptional regulatory systems, which cannot be explained by the abundance changes in known B. cenocepacia glycoproteins.IMPORTANCE Protein glycosylation is increasingly recognized as a common posttranslational protein modification in bacterial species.
31722994	10	56	theme	Burkholderia	1938:1949	arg1	pathogen					1929:1936	the opportunistic pathogen Burkholderia cenocepacia	1911:1961	the opportunistic pathogen Burkholderia cenocepacia	1911:1961	In this work, we investigated the effect of the disruption of O-linked glycosylation in the opportunistic pathogen Burkholderia cenocepacia using a combination of proteomic, molecular, and phenotypic assays.
31722994	0	57	theme	Glycosylation	25:37	arg1	Loss					0:3	Loss	0:3	Loss of O-Linked Protein Glycosylation in Burkholderia cenocepacia	0:65	Loss of O-Linked Protein Glycosylation in Burkholderia cenocepacia Impairs Biofilm Formation and Siderophore Activity and Alters Transcriptional Regulators.
31722994	13	58	theme	dramatic	2472:2479	arg1	changes					2492:2498	dramatic phenotypic changes	2472:2498	dramatic phenotypic changes in glycosylation-null strains, which are paralleled by both global proteomic and transcriptional alterations, which do not appear to directly result from the loss of glycosylation per se	2472:2685	These alterations lead to dramatic phenotypic changes in glycosylation-null strains, which are paralleled by both global proteomic and transcriptional alterations, which do not appear to directly result from the loss of glycosylation per se.
31722994	8	59	theme	posttranslational	1620:1636	arg1	modification					1646:1657	a common posttranslational protein modification	1611:1657	a common posttranslational protein modification in bacterial species	1611:1678	Together, our results show that loss of glycosylation in B. cenocepacia results in a global cell reprogramming via alteration of the transcriptional regulatory systems, which cannot be explained by the abundance changes in known B. cenocepacia glycoproteins.IMPORTANCE Protein glycosylation is increasingly recognized as a common posttranslational protein modification in bacterial species.
31722994	10	60	theme	opportunistic	1915:1927	arg1	pathogen					1929:1936	the opportunistic pathogen Burkholderia cenocepacia	1911:1961	the opportunistic pathogen Burkholderia cenocepacia	1911:1961	In this work, we investigated the effect of the disruption of O-linked glycosylation in the opportunistic pathogen Burkholderia cenocepacia using a combination of proteomic, molecular, and phenotypic assays.
31722994	8	61	theme	transcriptional	1423:1437	arg1	systems					1450:1456	the transcriptional regulatory systems	1419:1456	the transcriptional regulatory systems	1419:1456	Together, our results show that loss of glycosylation in B. cenocepacia results in a global cell reprogramming via alteration of the transcriptional regulatory systems, which cannot be explained by the abundance changes in known B. cenocepacia glycoproteins.IMPORTANCE Protein glycosylation is increasingly recognized as a common posttranslational protein modification in bacterial species.
31722994	14	62	link	O-linked	2738:2745	arg1	glycosylation					2747:2759	O-linked glycosylation	2738:2759	O-linked glycosylation	2738:2759	This research unravels the pleiotropic effects of O-linked glycosylation in B. cenocepacia, demonstrating that its loss does not simply affect the stability of the glycoproteome, but also interferes with transcription and the broader proteome.
31722994	6	63	located	found	1086:1090	arg1	amounts					1103:1109	reduced amounts	1095:1109	reduced amounts	1095:1109	Surprisingly, the abundance of most of the known glycosylated proteins did not significantly change in the glycosylation-defective mutants, except for BCAL1086 and BCAL2974, which were found in reduced amounts, suggesting they could be degraded.
31722994	6	63	located	found	1086:1090	arg2	BCAL2974					1065:1072	BCAL2974	1065:1072	BCAL2974	1065:1072	Surprisingly, the abundance of most of the known glycosylated proteins did not significantly change in the glycosylation-defective mutants, except for BCAL1086 and BCAL2974, which were found in reduced amounts, suggesting they could be degraded.
31722994	6	63	located	found	1086:1090	arg2	BCAL1086					1052:1059	BCAL1086	1052:1059	BCAL1086	1052:1059	Surprisingly, the abundance of most of the known glycosylated proteins did not significantly change in the glycosylation-defective mutants, except for BCAL1086 and BCAL2974, which were found in reduced amounts, suggesting they could be degraded.
31722994	11	64	theme	glycoproteins	2226:2238	arg1	stabilization					2204:2216	proteome stabilization	2195:2216	proteome stabilization of most glycoproteins	2195:2238	We find that in contrast to recent findings on the N-linked glycosylation systems of Campylobacter jejuni, O-linked glycosylation does not appear to play a role in proteome stabilization of most glycoproteins.
31722994	2	65	theme	β-Gal-	272:277	arg1	-β-GalNAc					298:306	the trisaccharide β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc	254:306	the trisaccharide β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc	254:306	The addition of the trisaccharide β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc to membrane exported proteins in Burkholderia cenocepacia is required for bacterial fitness and resistance to environmental stress.
31722994	8	66	theme	systems	1450:1456	arg1	alteration					1405:1414	alteration	1405:1414	alteration of the transcriptional regulatory systems, which cannot be explained by the abundance changes in known B. cenocepacia glycoproteins.IMPORTANCE Protein glycosylation is increasingly recognized as a common posttranslational protein modification in bacterial species	1405:1678	Together, our results show that loss of glycosylation in B. cenocepacia results in a global cell reprogramming via alteration of the transcriptional regulatory systems, which cannot be explained by the abundance changes in known B. cenocepacia glycoproteins.IMPORTANCE Protein glycosylation is increasingly recognized as a common posttranslational protein modification in bacterial species.
31722994	8	67	theme	bacterial	1662:1670	arg1	species					1672:1678	bacterial species	1662:1678	bacterial species	1662:1678	Together, our results show that loss of glycosylation in B. cenocepacia results in a global cell reprogramming via alteration of the transcriptional regulatory systems, which cannot be explained by the abundance changes in known B. cenocepacia glycoproteins.IMPORTANCE Protein glycosylation is increasingly recognized as a common posttranslational protein modification in bacterial species.
31722994	2	68	theme	bacterial	382:390	arg1	fitness					392:398	bacterial fitness	382:398	bacterial fitness	382:398	The addition of the trisaccharide β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc to membrane exported proteins in Burkholderia cenocepacia is required for bacterial fitness and resistance to environmental stress.
31722994	8	69	dep	glycosylation	1567:1579	arg1	recognized					1597:1606	recognized	1597:1606	is increasingly recognized as a common posttranslational protein modification in bacterial species	1581:1678	Together, our results show that loss of glycosylation in B. cenocepacia results in a global cell reprogramming via alteration of the transcriptional regulatory systems, which cannot be explained by the abundance changes in known B. cenocepacia glycoproteins.IMPORTANCE Protein glycosylation is increasingly recognized as a common posttranslational protein modification in bacterial species.
31722994	6	70	theme	glycosylated	950:961	arg1	proteins					963:970	the known glycosylated proteins	940:970	the known glycosylated proteins	940:970	Surprisingly, the abundance of most of the known glycosylated proteins did not significantly change in the glycosylation-defective mutants, except for BCAL1086 and BCAL2974, which were found in reduced amounts, suggesting they could be degraded.
31722994	10	71	theme	molecular	1997:2005	arg1	assays					2023:2028	proteomic, molecular, and phenotypic assays	1986:2028	proteomic, molecular, and phenotypic assays	1986:2028	In this work, we investigated the effect of the disruption of O-linked glycosylation in the opportunistic pathogen Burkholderia cenocepacia using a combination of proteomic, molecular, and phenotypic assays.
31722994	3	72	theme	glycosylation	513:525	arg1	absence					502:508	the absence	498:508	the absence of glycosylation	498:525	However, the underlying causes of the defects observed in the absence of glycosylation are unclear.
31722994	11	73	theme	proteome	2195:2202	arg1	stabilization					2204:2216	proteome stabilization	2195:2216	proteome stabilization of most glycoproteins	2195:2238	We find that in contrast to recent findings on the N-linked glycosylation systems of Campylobacter jejuni, O-linked glycosylation does not appear to play a role in proteome stabilization of most glycoproteins.
31722994	4	74	theme	DNA	590:592	arg1	cross-linking					594:606	DNA cross-linking	590:606	DNA cross-linking	590:606	Using proteomics, luciferase reporter assays, and DNA cross-linking, we demonstrate the loss of glycosylation leads to changes in transcriptional regulation of multiple proteins, including the repression of the master quorum CepR/I.
31722994	10	75	theme	proteomic	1986:1994	arg1	assays					2023:2028	proteomic, molecular, and phenotypic assays	1986:2028	proteomic, molecular, and phenotypic assays	1986:2028	In this work, we investigated the effect of the disruption of O-linked glycosylation in the opportunistic pathogen Burkholderia cenocepacia using a combination of proteomic, molecular, and phenotypic assays.
31722994	12	76	theme	glycosylation	2273:2285	arg1	loss					2265:2268	loss	2265:2268	loss of glycosylation in B. cenocepacia strains	2265:2311	Our results reveal that loss of glycosylation in B. cenocepacia strains leads to global proteome and transcriptional changes, including the repression of the quorum-sensing regulator cepR (BCAM1868) gene.
31722994	11	77	theme	N-linked	2082:2089	arg1	systems					2105:2111	the N-linked glycosylation systems	2078:2111	the N-linked glycosylation systems of Campylobacter jejuni	2078:2135	We find that in contrast to recent findings on the N-linked glycosylation systems of Campylobacter jejuni, O-linked glycosylation does not appear to play a role in proteome stabilization of most glycoproteins.
31722994	12	78	theme	quorum-sensing	2399:2412	arg1	gene					2440:2443	the quorum-sensing regulator cepR (BCAM1868) gene	2395:2443	the quorum-sensing regulator cepR (BCAM1868) gene	2395:2443	Our results reveal that loss of glycosylation in B. cenocepacia strains leads to global proteome and transcriptional changes, including the repression of the quorum-sensing regulator cepR (BCAM1868) gene.
31722994	11	79	theme	Campylobacter	2116:2128	arg1	jejuni					2130:2135	Campylobacter jejuni	2116:2135	Campylobacter jejuni	2116:2135	We find that in contrast to recent findings on the N-linked glycosylation systems of Campylobacter jejuni, O-linked glycosylation does not appear to play a role in proteome stabilization of most glycoproteins.
31722994	6	80	theme	most	932:935	arg1	abundance					919:927	the abundance	915:927	the abundance of most of the known glycosylated proteins	915:970	Surprisingly, the abundance of most of the known glycosylated proteins did not significantly change in the glycosylation-defective mutants, except for BCAL1086 and BCAL2974, which were found in reduced amounts, suggesting they could be degraded.
31722994	9	81	theme	role	1732:1735	arg1	understanding					1711:1723	our understanding	1707:1723	our understanding of the role of most glycosylation systems in bacterial physiology and pathogenesis	1707:1806	Despite this commonality, our understanding of the role of most glycosylation systems in bacterial physiology and pathogenesis is incomplete.
31722994	10	82	theme	assays	2023:2028	arg1	combination					1971:1981	a combination	1969:1981	a combination of proteomic, molecular, and phenotypic assays	1969:2028	In this work, we investigated the effect of the disruption of O-linked glycosylation in the opportunistic pathogen Burkholderia cenocepacia using a combination of proteomic, molecular, and phenotypic assays.
31722994	1	83	theme	conserved	193:201	arg1	glycosylation					174:186	O-linked protein glycosylation	157:186	O-linked protein glycosylation	157:186	O-linked protein glycosylation is a conserved feature of the Burkholderia genus.
31722994	1	83	theme	conserved	193:201	arg1	feature					203:209	a conserved feature	191:209	a conserved feature of the Burkholderia genus	191:235	O-linked protein glycosylation is a conserved feature of the Burkholderia genus.
31722994	9	84	theme	most	1740:1743	arg1	systems					1759:1765	most glycosylation systems	1740:1765	most glycosylation systems	1740:1765	Despite this commonality, our understanding of the role of most glycosylation systems in bacterial physiology and pathogenesis is incomplete.
31722994	2	85	theme	exported	320:327	arg1	proteins					329:336	membrane exported proteins	311:336	membrane exported proteins in Burkholderia cenocepacia	311:364	The addition of the trisaccharide β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc to membrane exported proteins in Burkholderia cenocepacia is required for bacterial fitness and resistance to environmental stress.
31722994	6	86	theme	glycosylation-defective	1008:1030	arg1	mutants					1032:1038	the glycosylation-defective mutants	1004:1038	the glycosylation-defective mutants	1004:1038	Surprisingly, the abundance of most of the known glycosylated proteins did not significantly change in the glycosylation-defective mutants, except for BCAL1086 and BCAL2974, which were found in reduced amounts, suggesting they could be degraded.
31722994	9	87	theme	systems	1759:1765	arg1	role					1732:1735	the role	1728:1735	the role of most glycosylation systems in bacterial physiology and pathogenesis	1728:1806	Despite this commonality, our understanding of the role of most glycosylation systems in bacterial physiology and pathogenesis is incomplete.
31722994	13	88	theme	transcriptional	2581:2595	arg1	alterations					2597:2607	both global proteomic and transcriptional alterations	2555:2607	both global proteomic and transcriptional alterations	2555:2607	These alterations lead to dramatic phenotypic changes in glycosylation-null strains, which are paralleled by both global proteomic and transcriptional alterations, which do not appear to directly result from the loss of glycosylation per se.
31722994	3	89	located	observed	486:493	arg1	absence					502:508	the absence	498:508	the absence of glycosylation	498:525	However, the underlying causes of the defects observed in the absence of glycosylation are unclear.
31722994	3	89	located	observed	486:493	arg2	defects					478:484	the defects	474:484	the defects observed in the absence of glycosylation	474:525	However, the underlying causes of the defects observed in the absence of glycosylation are unclear.
31722994	1	90	theme	Burkholderia	218:229	arg1	genus					231:235	the Burkholderia genus	214:235	the Burkholderia genus	214:235	O-linked protein glycosylation is a conserved feature of the Burkholderia genus.
31722994	11	91	theme	jejuni	2130:2135	arg1	systems					2105:2111	the N-linked glycosylation systems	2078:2111	the N-linked glycosylation systems of Campylobacter jejuni	2078:2135	We find that in contrast to recent findings on the N-linked glycosylation systems of Campylobacter jejuni, O-linked glycosylation does not appear to play a role in proteome stabilization of most glycoproteins.
31722994	14	92	theme	O-linked	2738:2745	arg1	glycosylation					2747:2759	O-linked glycosylation	2738:2759	O-linked glycosylation	2738:2759	This research unravels the pleiotropic effects of O-linked glycosylation in B. cenocepacia, demonstrating that its loss does not simply affect the stability of the glycoproteome, but also interferes with transcription and the broader proteome.
31722994	9	93	theme	bacterial	1770:1778	arg1	physiology					1780:1789	bacterial physiology	1770:1789	bacterial physiology	1770:1789	Despite this commonality, our understanding of the role of most glycosylation systems in bacterial physiology and pathogenesis is incomplete.
31722994	12	94	theme	BCAM1868	2430:2437	arg1	gene					2440:2443	the quorum-sensing regulator cepR (BCAM1868) gene	2395:2443	the quorum-sensing regulator cepR (BCAM1868) gene	2395:2443	Our results reveal that loss of glycosylation in B. cenocepacia strains leads to global proteome and transcriptional changes, including the repression of the quorum-sensing regulator cepR (BCAM1868) gene.
31722994	14	95	theme	broader	2914:2920	arg1	proteome					2922:2929	the broader proteome	2910:2929	the broader proteome	2910:2929	This research unravels the pleiotropic effects of O-linked glycosylation in B. cenocepacia, demonstrating that its loss does not simply affect the stability of the glycoproteome, but also interferes with transcription and the broader proteome.
31722994	4	96	theme	glycosylation	636:648	arg1	loss					628:631	the loss	624:631	the loss of glycosylation	624:648	Using proteomics, luciferase reporter assays, and DNA cross-linking, we demonstrate the loss of glycosylation leads to changes in transcriptional regulation of multiple proteins, including the repression of the master quorum CepR/I.
31722994	2	97	theme	trisaccharide	258:270	arg1	-β-GalNAc					298:306	the trisaccharide β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc	254:306	the trisaccharide β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc	254:306	The addition of the trisaccharide β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc to membrane exported proteins in Burkholderia cenocepacia is required for bacterial fitness and resistance to environmental stress.
31722994	11	98	theme	O-linked	2138:2145	arg1	glycosylation					2147:2159	O-linked glycosylation	2138:2159	O-linked glycosylation	2138:2159	We find that in contrast to recent findings on the N-linked glycosylation systems of Campylobacter jejuni, O-linked glycosylation does not appear to play a role in proteome stabilization of most glycoproteins.
31722994	12	99	theme	gene	2440:2443	arg1	repression					2381:2390	the repression	2377:2390	the repression of the quorum-sensing regulator cepR (BCAM1868) gene	2377:2443	Our results reveal that loss of glycosylation in B. cenocepacia strains leads to global proteome and transcriptional changes, including the repression of the quorum-sensing regulator cepR (BCAM1868) gene.
31722994	14	100	from	effects	2727:2733	arg1	cenocepacia					2767:2777	B. cenocepacia	2764:2777	B. cenocepacia	2764:2777	This research unravels the pleiotropic effects of O-linked glycosylation in B. cenocepacia, demonstrating that its loss does not simply affect the stability of the glycoproteome, but also interferes with transcription and the broader proteome.
31722994	8	101	theme	cenocepacia	1522:1532	arg1	glycosylation					1567:1579	known B. cenocepacia glycoproteins.IMPORTANCE Protein glycosylation	1513:1579	known B. cenocepacia glycoproteins.IMPORTANCE Protein glycosylation is increasingly recognized as a common posttranslational protein modification in bacterial species	1513:1678	Together, our results show that loss of glycosylation in B. cenocepacia results in a global cell reprogramming via alteration of the transcriptional regulatory systems, which cannot be explained by the abundance changes in known B. cenocepacia glycoproteins.IMPORTANCE Protein glycosylation is increasingly recognized as a common posttranslational protein modification in bacterial species.
31722994	9	102	from	understanding	1711:1723	arg1	physiology					1780:1789	bacterial physiology	1770:1789	bacterial physiology	1770:1789	Despite this commonality, our understanding of the role of most glycosylation systems in bacterial physiology and pathogenesis is incomplete.
31722994	9	102	from	understanding	1711:1723	arg1	pathogenesis					1795:1806	pathogenesis	1795:1806	pathogenesis	1795:1806	Despite this commonality, our understanding of the role of most glycosylation systems in bacterial physiology and pathogenesis is incomplete.
31722994	8	103	theme	abundance	1492:1500	arg1	changes					1502:1508	the abundance changes	1488:1508	the abundance changes in known B. cenocepacia glycoproteins.IMPORTANCE Protein glycosylation is increasingly recognized as a common posttranslational protein modification in bacterial species	1488:1678	Together, our results show that loss of glycosylation in B. cenocepacia results in a global cell reprogramming via alteration of the transcriptional regulatory systems, which cannot be explained by the abundance changes in known B. cenocepacia glycoproteins.IMPORTANCE Protein glycosylation is increasingly recognized as a common posttranslational protein modification in bacterial species.
31722994	7	104	theme	proteins	1178:1185	arg1	loss					1160:1163	the loss	1156:1163	the loss of these two proteins	1156:1185	However, the loss of these two proteins was not responsible for driving the proteomic alterations, biofilm formation, or siderophore activity.
31722994	7	104	theme	proteins	1178:1185	arg1	responsible					1195:1205	responsible	1195:1205	responsible	1195:1205	However, the loss of these two proteins was not responsible for driving the proteomic alterations, biofilm formation, or siderophore activity.
31722994	13	105	theme	proteomic	2567:2575	arg1	alterations					2597:2607	both global proteomic and transcriptional alterations	2555:2607	both global proteomic and transcriptional alterations	2555:2607	These alterations lead to dramatic phenotypic changes in glycosylation-null strains, which are paralleled by both global proteomic and transcriptional alterations, which do not appear to directly result from the loss of glycosylation per se.
31722994	11	106	theme	recent	2059:2064	arg1	findings					2066:2073	recent findings	2059:2073	recent findings on the N-linked glycosylation systems of Campylobacter jejuni	2059:2135	We find that in contrast to recent findings on the N-linked glycosylation systems of Campylobacter jejuni, O-linked glycosylation does not appear to play a role in proteome stabilization of most glycoproteins.
31722994	5	107	from	defects	868:874	arg1	activity					891:898	siderophore activity	879:898	siderophore activity	879:898	These proteomic and transcriptional alterations lead to the abolition of biofilm formation and defects in siderophore activity.
31722994	12	108	theme	cepR	2424:2427	arg1	gene					2440:2443	the quorum-sensing regulator cepR (BCAM1868) gene	2395:2443	the quorum-sensing regulator cepR (BCAM1868) gene	2395:2443	Our results reveal that loss of glycosylation in B. cenocepacia strains leads to global proteome and transcriptional changes, including the repression of the quorum-sensing regulator cepR (BCAM1868) gene.
31722994	7	109	theme	biofilm	1246:1252	arg1	formation					1254:1262	biofilm formation	1246:1262	biofilm formation	1246:1262	However, the loss of these two proteins was not responsible for driving the proteomic alterations, biofilm formation, or siderophore activity.
31722994	10	110	theme	glycosylation	1894:1906	arg1	disruption					1871:1880	the disruption	1867:1880	the disruption of O-linked glycosylation in the opportunistic pathogen Burkholderia cenocepacia	1867:1961	In this work, we investigated the effect of the disruption of O-linked glycosylation in the opportunistic pathogen Burkholderia cenocepacia using a combination of proteomic, molecular, and phenotypic assays.
31722994	1	111	theme	protein	166:172	arg1	glycosylation					174:186	O-linked protein glycosylation	157:186	O-linked protein glycosylation	157:186	O-linked protein glycosylation is a conserved feature of the Burkholderia genus.
31722994	1	111	theme	protein	166:172	arg1	feature					203:209	a conserved feature	191:209	a conserved feature of the Burkholderia genus	191:235	O-linked protein glycosylation is a conserved feature of the Burkholderia genus.
31722994	9	112	from	pathogenesis	1795:1806	arg1	understanding					1711:1723	our understanding	1707:1723	our understanding of the role of most glycosylation systems in bacterial physiology and pathogenesis	1707:1806	Despite this commonality, our understanding of the role of most glycosylation systems in bacterial physiology and pathogenesis is incomplete.
31722994	8	113	theme	B.	1519:1520	arg1	glycosylation					1567:1579	known B. cenocepacia glycoproteins.IMPORTANCE Protein glycosylation	1513:1579	known B. cenocepacia glycoproteins.IMPORTANCE Protein glycosylation is increasingly recognized as a common posttranslational protein modification in bacterial species	1513:1678	Together, our results show that loss of glycosylation in B. cenocepacia results in a global cell reprogramming via alteration of the transcriptional regulatory systems, which cannot be explained by the abundance changes in known B. cenocepacia glycoproteins.IMPORTANCE Protein glycosylation is increasingly recognized as a common posttranslational protein modification in bacterial species.
31722994	4	114	theme	multiple	700:707	arg1	repression					733:742	the repression	729:742	the repression of the master quorum CepR/I	729:770	Using proteomics, luciferase reporter assays, and DNA cross-linking, we demonstrate the loss of glycosylation leads to changes in transcriptional regulation of multiple proteins, including the repression of the master quorum CepR/I.
31722994	4	114	theme	multiple	700:707	arg1	proteins					709:716	multiple proteins	700:716	multiple proteins	700:716	Using proteomics, luciferase reporter assays, and DNA cross-linking, we demonstrate the loss of glycosylation leads to changes in transcriptional regulation of multiple proteins, including the repression of the master quorum CepR/I.
31722994	8	115	from	changes	1502:1508	arg1	glycosylation					1567:1579	known B. cenocepacia glycoproteins.IMPORTANCE Protein glycosylation	1513:1579	known B. cenocepacia glycoproteins.IMPORTANCE Protein glycosylation is increasingly recognized as a common posttranslational protein modification in bacterial species	1513:1678	Together, our results show that loss of glycosylation in B. cenocepacia results in a global cell reprogramming via alteration of the transcriptional regulatory systems, which cannot be explained by the abundance changes in known B. cenocepacia glycoproteins.IMPORTANCE Protein glycosylation is increasingly recognized as a common posttranslational protein modification in bacterial species.
31722994	9	116	from	physiology	1780:1789	arg1	understanding					1711:1723	our understanding	1707:1723	our understanding of the role of most glycosylation systems in bacterial physiology and pathogenesis	1707:1806	Despite this commonality, our understanding of the role of most glycosylation systems in bacterial physiology and pathogenesis is incomplete.
31722994	8	117	theme	glycoproteins.IMPORTANCE	1534:1557	arg1	glycosylation					1567:1579	known B. cenocepacia glycoproteins.IMPORTANCE Protein glycosylation	1513:1579	known B. cenocepacia glycoproteins.IMPORTANCE Protein glycosylation is increasingly recognized as a common posttranslational protein modification in bacterial species	1513:1678	Together, our results show that loss of glycosylation in B. cenocepacia results in a global cell reprogramming via alteration of the transcriptional regulatory systems, which cannot be explained by the abundance changes in known B. cenocepacia glycoproteins.IMPORTANCE Protein glycosylation is increasingly recognized as a common posttranslational protein modification in bacterial species.
31722994	10	118	link	O-linked	1885:1892	arg1	glycosylation					1894:1906	O-linked glycosylation	1885:1906	O-linked glycosylation	1885:1906	In this work, we investigated the effect of the disruption of O-linked glycosylation in the opportunistic pathogen Burkholderia cenocepacia using a combination of proteomic, molecular, and phenotypic assays.
31722994	12	119	theme	transcriptional	2342:2356	arg1	changes					2358:2364	global proteome and transcriptional changes	2322:2364	global proteome and transcriptional changes	2322:2364	Our results reveal that loss of glycosylation in B. cenocepacia strains leads to global proteome and transcriptional changes, including the repression of the quorum-sensing regulator cepR (BCAM1868) gene.
31722994	12	119	theme	transcriptional	2342:2356	arg1	repression					2381:2390	the repression	2377:2390	the repression of the quorum-sensing regulator cepR (BCAM1868) gene	2377:2443	Our results reveal that loss of glycosylation in B. cenocepacia strains leads to global proteome and transcriptional changes, including the repression of the quorum-sensing regulator cepR (BCAM1868) gene.
31722994	14	120	theme	glycoproteome	2852:2864	arg1	stability					2835:2843	the stability	2831:2843	the stability of the glycoproteome	2831:2864	This research unravels the pleiotropic effects of O-linked glycosylation in B. cenocepacia, demonstrating that its loss does not simply affect the stability of the glycoproteome, but also interferes with transcription and the broader proteome.
31722994	12	121	theme	global	2322:2327	arg1	changes					2358:2364	global proteome and transcriptional changes	2322:2364	global proteome and transcriptional changes	2322:2364	Our results reveal that loss of glycosylation in B. cenocepacia strains leads to global proteome and transcriptional changes, including the repression of the quorum-sensing regulator cepR (BCAM1868) gene.
31722994	12	121	theme	global	2322:2327	arg1	repression					2381:2390	the repression	2377:2390	the repression of the quorum-sensing regulator cepR (BCAM1868) gene	2377:2443	Our results reveal that loss of glycosylation in B. cenocepacia strains leads to global proteome and transcriptional changes, including the repression of the quorum-sensing regulator cepR (BCAM1868) gene.
31722994	10	122	from	disruption	1871:1880	arg1	pathogen					1929:1936	the opportunistic pathogen Burkholderia cenocepacia	1911:1961	the opportunistic pathogen Burkholderia cenocepacia	1911:1961	In this work, we investigated the effect of the disruption of O-linked glycosylation in the opportunistic pathogen Burkholderia cenocepacia using a combination of proteomic, molecular, and phenotypic assays.
31722994	4	123	theme	master	751:756	arg1	CepR/I					765:770	the master quorum CepR/I	747:770	the master quorum CepR/I	747:770	Using proteomics, luciferase reporter assays, and DNA cross-linking, we demonstrate the loss of glycosylation leads to changes in transcriptional regulation of multiple proteins, including the repression of the master quorum CepR/I.
31722994	0	124	theme	Protein	17:23	arg1	Glycosylation					25:37	O-Linked Protein Glycosylation	8:37	O-Linked Protein Glycosylation	8:37	Loss of O-Linked Protein Glycosylation in Burkholderia cenocepacia Impairs Biofilm Formation and Siderophore Activity and Alters Transcriptional Regulators.
31722994	8	125	theme	common	1613:1618	arg1	modification					1646:1657	a common posttranslational protein modification	1611:1657	a common posttranslational protein modification in bacterial species	1611:1678	Together, our results show that loss of glycosylation in B. cenocepacia results in a global cell reprogramming via alteration of the transcriptional regulatory systems, which cannot be explained by the abundance changes in known B. cenocepacia glycoproteins.IMPORTANCE Protein glycosylation is increasingly recognized as a common posttranslational protein modification in bacterial species.
31722994	4	126	theme	CepR/I	765:770	arg1	repression					733:742	the repression	729:742	the repression of the master quorum CepR/I	729:770	Using proteomics, luciferase reporter assays, and DNA cross-linking, we demonstrate the loss of glycosylation leads to changes in transcriptional regulation of multiple proteins, including the repression of the master quorum CepR/I.
31722994	8	127	theme	protein	1638:1644	arg1	modification					1646:1657	a common posttranslational protein modification	1611:1657	a common posttranslational protein modification in bacterial species	1611:1678	Together, our results show that loss of glycosylation in B. cenocepacia results in a global cell reprogramming via alteration of the transcriptional regulatory systems, which cannot be explained by the abundance changes in known B. cenocepacia glycoproteins.IMPORTANCE Protein glycosylation is increasingly recognized as a common posttranslational protein modification in bacterial species.
31722994	2	128	theme	environmental	418:430	arg1	stress					432:437	environmental stress	418:437	environmental stress	418:437	The addition of the trisaccharide β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc to membrane exported proteins in Burkholderia cenocepacia is required for bacterial fitness and resistance to environmental stress.
31722994	13	129	theme	glycosylation	2666:2678	arg1	loss					2658:2661	the loss	2654:2661	the loss of glycosylation	2654:2678	These alterations lead to dramatic phenotypic changes in glycosylation-null strains, which are paralleled by both global proteomic and transcriptional alterations, which do not appear to directly result from the loss of glycosylation per se.
31722994	13	130	theme	phenotypic	2481:2490	arg1	changes					2492:2498	dramatic phenotypic changes	2472:2498	dramatic phenotypic changes in glycosylation-null strains, which are paralleled by both global proteomic and transcriptional alterations, which do not appear to directly result from the loss of glycosylation per se	2472:2685	These alterations lead to dramatic phenotypic changes in glycosylation-null strains, which are paralleled by both global proteomic and transcriptional alterations, which do not appear to directly result from the loss of glycosylation per se.
31722994	4	131	theme	luciferase	558:567	arg1	assays					578:583	luciferase reporter assays	558:583	luciferase reporter assays	558:583	Using proteomics, luciferase reporter assays, and DNA cross-linking, we demonstrate the loss of glycosylation leads to changes in transcriptional regulation of multiple proteins, including the repression of the master quorum CepR/I.
31722994	1	132	link	O-linked	157:164	arg1	glycosylation					174:186	O-linked protein glycosylation	157:186	O-linked protein glycosylation	157:186	O-linked protein glycosylation is a conserved feature of the Burkholderia genus.
31722994	1	132	link	O-linked	157:164	arg1	feature					203:209	a conserved feature	191:209	a conserved feature of the Burkholderia genus	191:235	O-linked protein glycosylation is a conserved feature of the Burkholderia genus.
31722994	10	133	theme	disruption	1871:1880	arg1	effect					1857:1862	the effect	1853:1862	the effect of the disruption of O-linked glycosylation in the opportunistic pathogen Burkholderia cenocepacia	1853:1961	In this work, we investigated the effect of the disruption of O-linked glycosylation in the opportunistic pathogen Burkholderia cenocepacia using a combination of proteomic, molecular, and phenotypic assays.
31722994	8	134	theme	regulatory	1439:1448	arg1	systems					1450:1456	the transcriptional regulatory systems	1419:1456	the transcriptional regulatory systems	1419:1456	Together, our results show that loss of glycosylation in B. cenocepacia results in a global cell reprogramming via alteration of the transcriptional regulatory systems, which cannot be explained by the abundance changes in known B. cenocepacia glycoproteins.IMPORTANCE Protein glycosylation is increasingly recognized as a common posttranslational protein modification in bacterial species.
31722994	12	135	from	loss	2265:2268	arg1	strains					2305:2311	B. cenocepacia strains	2290:2311	B. cenocepacia strains	2290:2311	Our results reveal that loss of glycosylation in B. cenocepacia strains leads to global proteome and transcriptional changes, including the repression of the quorum-sensing regulator cepR (BCAM1868) gene.
31722994	10	136	from	pathogen	1929:1936	arg1	effect					1857:1862	the effect	1853:1862	the effect of the disruption of O-linked glycosylation in the opportunistic pathogen Burkholderia cenocepacia	1853:1961	In this work, we investigated the effect of the disruption of O-linked glycosylation in the opportunistic pathogen Burkholderia cenocepacia using a combination of proteomic, molecular, and phenotypic assays.
31722994	7	137	theme	siderophore	1268:1278	arg1	activity					1280:1287	siderophore activity	1268:1287	siderophore activity	1268:1287	However, the loss of these two proteins was not responsible for driving the proteomic alterations, biofilm formation, or siderophore activity.
31722994	11	138	link	O-linked	2138:2145	arg1	glycosylation					2147:2159	O-linked glycosylation	2138:2159	O-linked glycosylation	2138:2159	We find that in contrast to recent findings on the N-linked glycosylation systems of Campylobacter jejuni, O-linked glycosylation does not appear to play a role in proteome stabilization of most glycoproteins.
31722994	12	139	theme	B.	2290:2291	arg1	strains					2305:2311	B. cenocepacia strains	2290:2311	B. cenocepacia strains	2290:2311	Our results reveal that loss of glycosylation in B. cenocepacia strains leads to global proteome and transcriptional changes, including the repression of the quorum-sensing regulator cepR (BCAM1868) gene.
29469653	2	0	theme	extensive	313:321	arg1	assessment					340:349	extensive 3-way similarity assessment	313:349	extensive 3-way similarity assessment	313:349	To demonstrate biosimilarity of CT-P10 with the reference medicinal product (RMP), extensive 3-way similarity assessment has been conducted between CT-P10, EU-Rituximab and US-Rituximab, focusing on the physicochemical and biological quality attributes.
29469653	7	1	from	effect	1258:1263	arg1	affinity					1350:1357	Fc receptor binding affinity	1330:1357	Fc receptor binding affinity	1330:1357	Nevertheless, the effect of the observed level of afucosylation in CT-P10 drug product on Fc receptor binding affinity or antibody-dependent cell-mediated cytotoxicity was found to be negligible based on the spiking study with highly afucosylated sample.
29469653	7	1	from	effect	1258:1263	arg1	cytotoxicity					1395:1406	antibody-dependent cell-mediated cytotoxicity	1362:1406	antibody-dependent cell-mediated cytotoxicity	1362:1406	Nevertheless, the effect of the observed level of afucosylation in CT-P10 drug product on Fc receptor binding affinity or antibody-dependent cell-mediated cytotoxicity was found to be negligible based on the spiking study with highly afucosylated sample.
29469653	8	2	theme	RMPs	1675:1678	arg1	range					1666:1670	the quality range	1654:1670	the quality range of RMPs	1654:1678	Arrays of biological assays representative of known and putative mechanisms of action for rituximab have shown that biological activities of CT-P10 are within the quality range of RMPs.
29469653	2	3	theme	CT-P10	262:267	arg1	biosimilarity					245:257	biosimilarity	245:257	biosimilarity of CT-P10 with the reference medicinal product (RMP)	245:310	To demonstrate biosimilarity of CT-P10 with the reference medicinal product (RMP), extensive 3-way similarity assessment has been conducted between CT-P10, EU-Rituximab and US-Rituximab, focusing on the physicochemical and biological quality attributes.
29469653	6	4	with	proportion	1114:1123	arg1	RMPs					1134:1137	the RMPs	1130:1137	the RMPs	1130:1137	Oligosaccharide profiling has revealed that, although CT-P10 contains the same conserved glycan species and relative proportion with the RMPs, the content of total afucosylated glycan in CT-P10 was slightly higher than in EU- or US-Rituximab.
29469653	9	5	theme	clinical	1699:1706	arg1	studies					1708:1714	clinical studies	1699:1714	clinical studies	1699:1714	Recent results of clinical studies have further confirmed that the CT-P10 exhibits equivalent clinical efficacy and safety profiles compared to EU- and US-Rituximab.
29469653	1	6	theme	biosimilar	167:176	arg1	product					178:184	the world's first rituximab biosimilar product	139:184	the world's first rituximab biosimilar product	139:184	CT-P10 (Truxima™) was recently approved as the world's first rituximab biosimilar product in the European Union (EU) and South Korea.
29469653	10	7	from	similar	1970:1976	arg1	terms					2007:2011	terms	2007:2011	terms of physicochemical properties, biological activities, efficacy, and safety for its final approval as a biosimilar product	2007:2133	The current 3-way similarity assessment together with clinical study results confidently demonstrate that CT-P10 is highly similar with EU- and US-Rituximab in terms of physicochemical properties, biological activities, efficacy, and safety for its final approval as a biosimilar product.
29469653	7	8	theme	afucosylated	1474:1485	arg1	sample					1487:1492	highly afucosylated sample	1467:1492	highly afucosylated sample	1467:1492	Nevertheless, the effect of the observed level of afucosylation in CT-P10 drug product on Fc receptor binding affinity or antibody-dependent cell-mediated cytotoxicity was found to be negligible based on the spiking study with highly afucosylated sample.
29469653	7	9	from	level	1281:1285	arg1	product					1319:1325	CT-P10 drug product	1307:1325	CT-P10 drug product	1307:1325	Nevertheless, the effect of the observed level of afucosylation in CT-P10 drug product on Fc receptor binding affinity or antibody-dependent cell-mediated cytotoxicity was found to be negligible based on the spiking study with highly afucosylated sample.
29469653	4	10	with	comparable	789:798	arg1	those					805:809	those	805:809	those	805:809	Purity/impurity profiles of CT-P10 measured by the levels of aggregates, fragments, non-glycosylated form and process-related impurities were also found to be comparable with those of RMPs.
29469653	2	11	theme	medicinal	288:296	arg1	RMP					307:309	RMP	307:309	RMP	307:309	To demonstrate biosimilarity of CT-P10 with the reference medicinal product (RMP), extensive 3-way similarity assessment has been conducted between CT-P10, EU-Rituximab and US-Rituximab, focusing on the physicochemical and biological quality attributes.
29469653	2	11	theme	medicinal	288:296	arg1	product					298:304	the reference medicinal product	274:304	the reference medicinal product (RMP)	274:310	To demonstrate biosimilarity of CT-P10 with the reference medicinal product (RMP), extensive 3-way similarity assessment has been conducted between CT-P10, EU-Rituximab and US-Rituximab, focusing on the physicochemical and biological quality attributes.
29469653	5	12	theme	less	894:897	arg1	variant					925:931	slightly less N-terminal pyro-glutamate variant	885:931	slightly less N-terminal pyro-glutamate variant	885:931	In terms of the post-translational modification, CT-P10 contains slightly less N-terminal pyro-glutamate variant, which has been known not to affect product efficacy or safety.
29469653	7	13	from	afucosylation	1290:1302	arg1	product					1319:1325	CT-P10 drug product	1307:1325	CT-P10 drug product	1307:1325	Nevertheless, the effect of the observed level of afucosylation in CT-P10 drug product on Fc receptor binding affinity or antibody-dependent cell-mediated cytotoxicity was found to be negligible based on the spiking study with highly afucosylated sample.
29469653	6	14	theme	afucosylated	1161:1172	arg1	glycan					1174:1179	total afucosylated glycan	1155:1179	total afucosylated glycan	1155:1179	Oligosaccharide profiling has revealed that, although CT-P10 contains the same conserved glycan species and relative proportion with the RMPs, the content of total afucosylated glycan in CT-P10 was slightly higher than in EU- or US-Rituximab.
29469653	6	15	gly	afucosylated	1161:1172	arg1	glycan					1174:1179	total afucosylated glycan	1155:1179	total afucosylated glycan	1155:1179	Oligosaccharide profiling has revealed that, although CT-P10 contains the same conserved glycan species and relative proportion with the RMPs, the content of total afucosylated glycan in CT-P10 was slightly higher than in EU- or US-Rituximab.
29469653	10	16	theme	physicochemical	2016:2030	arg1	properties					2032:2041	physicochemical properties	2016:2041	physicochemical properties	2016:2041	The current 3-way similarity assessment together with clinical study results confidently demonstrate that CT-P10 is highly similar with EU- and US-Rituximab in terms of physicochemical properties, biological activities, efficacy, and safety for its final approval as a biosimilar product.
29469653	10	16	theme	physicochemical	2016:2030	arg1	safety					2081:2086	safety	2081:2086	safety	2081:2086	The current 3-way similarity assessment together with clinical study results confidently demonstrate that CT-P10 is highly similar with EU- and US-Rituximab in terms of physicochemical properties, biological activities, efficacy, and safety for its final approval as a biosimilar product.
29469653	10	16	theme	physicochemical	2016:2030	arg1	efficacy					2067:2074	efficacy	2067:2074	efficacy	2067:2074	The current 3-way similarity assessment together with clinical study results confidently demonstrate that CT-P10 is highly similar with EU- and US-Rituximab in terms of physicochemical properties, biological activities, efficacy, and safety for its final approval as a biosimilar product.
29469653	10	16	theme	physicochemical	2016:2030	arg1	activities					2055:2064	biological activities	2044:2064	biological activities	2044:2064	The current 3-way similarity assessment together with clinical study results confidently demonstrate that CT-P10 is highly similar with EU- and US-Rituximab in terms of physicochemical properties, biological activities, efficacy, and safety for its final approval as a biosimilar product.
29469653	4	17	gly	non-glycosylated	714:729	arg1	form					731:734	non-glycosylated form	714:734	non-glycosylated form	714:734	Purity/impurity profiles of CT-P10 measured by the levels of aggregates, fragments, non-glycosylated form and process-related impurities were also found to be comparable with those of RMPs.
29469653	3	18	theme	primary	560:566	arg1	structures					585:594	identical primary and higher order structures	550:594	identical primary and higher order structures	550:594	A multitude of state-of-the-art analyses revealed that CT-P10 has identical primary and higher order structures compared to the original product.
29469653	5	19	theme	post-translational	836:853	arg1	modification					855:866	the post-translational modification	832:866	the post-translational modification	832:866	In terms of the post-translational modification, CT-P10 contains slightly less N-terminal pyro-glutamate variant, which has been known not to affect product efficacy or safety.
29469653	7	20	theme	Fc	1330:1331	arg1	affinity					1350:1357	Fc receptor binding affinity	1330:1357	Fc receptor binding affinity	1330:1357	Nevertheless, the effect of the observed level of afucosylation in CT-P10 drug product on Fc receptor binding affinity or antibody-dependent cell-mediated cytotoxicity was found to be negligible based on the spiking study with highly afucosylated sample.
29469653	4	21	theme	CT-P10	658:663	arg1	profiles					646:653	Purity/impurity profiles	630:653	Purity/impurity profiles of CT-P10 measured by the levels of aggregates, fragments, non-glycosylated form and process-related impurities	630:765	Purity/impurity profiles of CT-P10 measured by the levels of aggregates, fragments, non-glycosylated form and process-related impurities were also found to be comparable with those of RMPs.
29469653	10	22	theme	current	1851:1857	arg1	assessment					1876:1885	The current 3-way similarity assessment	1847:1885	The current 3-way similarity assessment together with clinical study results	1847:1922	The current 3-way similarity assessment together with clinical study results confidently demonstrate that CT-P10 is highly similar with EU- and US-Rituximab in terms of physicochemical properties, biological activities, efficacy, and safety for its final approval as a biosimilar product.
29469653	1	23	from	Union	202:206	arg1	approved					127:134	approved	127:134	approved	127:134	CT-P10 (Truxima™) was recently approved as the world's first rituximab biosimilar product in the European Union (EU) and South Korea.
29469653	10	24	from	terms	2007:2011	arg1	similar					1970:1976	similar	1970:1976	similar	1970:1976	The current 3-way similarity assessment together with clinical study results confidently demonstrate that CT-P10 is highly similar with EU- and US-Rituximab in terms of physicochemical properties, biological activities, efficacy, and safety for its final approval as a biosimilar product.
29469653	4	25	theme	non-glycosylated	714:729	arg1	form					731:734	non-glycosylated form	714:734	non-glycosylated form	714:734	Purity/impurity profiles of CT-P10 measured by the levels of aggregates, fragments, non-glycosylated form and process-related impurities were also found to be comparable with those of RMPs.
29469653	9	26	theme	clinical	1775:1782	arg1	efficacy					1784:1791	equivalent clinical efficacy	1764:1791	equivalent clinical efficacy	1764:1791	Recent results of clinical studies have further confirmed that the CT-P10 exhibits equivalent clinical efficacy and safety profiles compared to EU- and US-Rituximab.
29469653	7	27	theme	antibody-dependent	1362:1379	arg1	cytotoxicity					1395:1406	antibody-dependent cell-mediated cytotoxicity	1362:1406	antibody-dependent cell-mediated cytotoxicity	1362:1406	Nevertheless, the effect of the observed level of afucosylation in CT-P10 drug product on Fc receptor binding affinity or antibody-dependent cell-mediated cytotoxicity was found to be negligible based on the spiking study with highly afucosylated sample.
29469653	2	28	with	biosimilarity	245:257	arg1	RMP					307:309	RMP	307:309	RMP	307:309	To demonstrate biosimilarity of CT-P10 with the reference medicinal product (RMP), extensive 3-way similarity assessment has been conducted between CT-P10, EU-Rituximab and US-Rituximab, focusing on the physicochemical and biological quality attributes.
29469653	2	28	with	biosimilarity	245:257	arg1	product					298:304	the reference medicinal product	274:304	the reference medicinal product (RMP)	274:310	To demonstrate biosimilarity of CT-P10 with the reference medicinal product (RMP), extensive 3-way similarity assessment has been conducted between CT-P10, EU-Rituximab and US-Rituximab, focusing on the physicochemical and biological quality attributes.
29469653	8	29	theme	biological	1505:1514	arg1	assays					1516:1521	biological assays	1505:1521	biological assays representative of known and putative mechanisms of action for rituximab	1505:1593	Arrays of biological assays representative of known and putative mechanisms of action for rituximab have shown that biological activities of CT-P10 are within the quality range of RMPs.
29469653	7	30	theme	drug	1314:1317	arg1	product					1319:1325	CT-P10 drug product	1307:1325	CT-P10 drug product	1307:1325	Nevertheless, the effect of the observed level of afucosylation in CT-P10 drug product on Fc receptor binding affinity or antibody-dependent cell-mediated cytotoxicity was found to be negligible based on the spiking study with highly afucosylated sample.
29469653	4	31	theme	aggregates	691:700	arg1	levels					681:686	the levels	677:686	the levels of aggregates, fragments, non-glycosylated form and process-related impurities	677:765	Purity/impurity profiles of CT-P10 measured by the levels of aggregates, fragments, non-glycosylated form and process-related impurities were also found to be comparable with those of RMPs.
29469653	0	32	theme	reference	67:75	arg1	product					87:93	reference medicinal product	67:93	reference medicinal product	67:93	Analytical similarity assessment of rituximab biosimilar CT-P10 to reference medicinal product.
29469653	3	33	theme	original	612:619	arg1	product					621:627	the original product	608:627	the original product	608:627	A multitude of state-of-the-art analyses revealed that CT-P10 has identical primary and higher order structures compared to the original product.
29469653	5	34	theme	pyro-glutamate	910:923	arg1	variant					925:931	slightly less N-terminal pyro-glutamate variant	885:931	slightly less N-terminal pyro-glutamate variant	885:931	In terms of the post-translational modification, CT-P10 contains slightly less N-terminal pyro-glutamate variant, which has been known not to affect product efficacy or safety.
29469653	8	35	theme	representative	1523:1536	arg1	assays					1516:1521	biological assays	1505:1521	biological assays representative of known and putative mechanisms of action for rituximab	1505:1593	Arrays of biological assays representative of known and putative mechanisms of action for rituximab have shown that biological activities of CT-P10 are within the quality range of RMPs.
29469653	4	36	theme	fragments	703:711	arg1	levels					681:686	the levels	677:686	the levels of aggregates, fragments, non-glycosylated form and process-related impurities	677:765	Purity/impurity profiles of CT-P10 measured by the levels of aggregates, fragments, non-glycosylated form and process-related impurities were also found to be comparable with those of RMPs.
29469653	1	37	theme	first	151:155	arg1	product					178:184	the world's first rituximab biosimilar product	139:184	the world's first rituximab biosimilar product	139:184	CT-P10 (Truxima™) was recently approved as the world's first rituximab biosimilar product in the European Union (EU) and South Korea.
29469653	8	38	theme	known	1541:1545	arg1	mechanisms					1560:1569	known and putative mechanisms	1541:1569	known and putative mechanisms of action for rituximab	1541:1593	Arrays of biological assays representative of known and putative mechanisms of action for rituximab have shown that biological activities of CT-P10 are within the quality range of RMPs.
29469653	10	39	theme	final	2096:2100	arg1	approval					2102:2109	its final approval	2092:2109	its final approval as a biosimilar product	2092:2133	The current 3-way similarity assessment together with clinical study results confidently demonstrate that CT-P10 is highly similar with EU- and US-Rituximab in terms of physicochemical properties, biological activities, efficacy, and safety for its final approval as a biosimilar product.
29469653	6	40	theme	Oligosaccharide	997:1011	arg1	profiling					1013:1021	Oligosaccharide profiling	997:1021	Oligosaccharide profiling	997:1021	Oligosaccharide profiling has revealed that, although CT-P10 contains the same conserved glycan species and relative proportion with the RMPs, the content of total afucosylated glycan in CT-P10 was slightly higher than in EU- or US-Rituximab.
29469653	8	41	theme	putative	1551:1558	arg1	mechanisms					1560:1569	known and putative mechanisms	1541:1569	known and putative mechanisms of action for rituximab	1541:1593	Arrays of biological assays representative of known and putative mechanisms of action for rituximab have shown that biological activities of CT-P10 are within the quality range of RMPs.
29469653	1	42	from	approved	127:134	arg1	EU					209:210	EU	209:210	EU	209:210	CT-P10 (Truxima™) was recently approved as the world's first rituximab biosimilar product in the European Union (EU) and South Korea.
29469653	1	42	from	approved	127:134	arg1	Union					202:206	the European Union	189:206	the European Union (EU)	189:211	CT-P10 (Truxima™) was recently approved as the world's first rituximab biosimilar product in the European Union (EU) and South Korea.
29469653	1	42	from	approved	127:134	arg1	Korea					223:227	Korea	223:227	Korea	223:227	CT-P10 (Truxima™) was recently approved as the world's first rituximab biosimilar product in the European Union (EU) and South Korea.
29469653	7	43	gly	afucosylated	1474:1485	arg1	sample					1487:1492	highly afucosylated sample	1467:1492	highly afucosylated sample	1467:1492	Nevertheless, the effect of the observed level of afucosylation in CT-P10 drug product on Fc receptor binding affinity or antibody-dependent cell-mediated cytotoxicity was found to be negligible based on the spiking study with highly afucosylated sample.
29469653	7	44	theme	binding	1342:1348	arg1	affinity					1350:1357	Fc receptor binding affinity	1330:1357	Fc receptor binding affinity	1330:1357	Nevertheless, the effect of the observed level of afucosylation in CT-P10 drug product on Fc receptor binding affinity or antibody-dependent cell-mediated cytotoxicity was found to be negligible based on the spiking study with highly afucosylated sample.
29469653	4	45	theme	impurities	756:765	arg1	levels					681:686	the levels	677:686	the levels of aggregates, fragments, non-glycosylated form and process-related impurities	677:765	Purity/impurity profiles of CT-P10 measured by the levels of aggregates, fragments, non-glycosylated form and process-related impurities were also found to be comparable with those of RMPs.
29469653	0	46	theme	Analytical	0:9	arg1	assessment					22:31	Analytical similarity assessment	0:31	Analytical similarity assessment of rituximab	0:44	Analytical similarity assessment of rituximab biosimilar CT-P10 to reference medicinal product.
29469653	6	47	theme	glycan	1086:1091	arg1	species					1093:1099	the same conserved glycan species	1067:1099	the same conserved glycan species	1067:1099	Oligosaccharide profiling has revealed that, although CT-P10 contains the same conserved glycan species and relative proportion with the RMPs, the content of total afucosylated glycan in CT-P10 was slightly higher than in EU- or US-Rituximab.
29469653	6	48	with	species	1093:1099	arg1	RMPs					1134:1137	the RMPs	1130:1137	the RMPs	1130:1137	Oligosaccharide profiling has revealed that, although CT-P10 contains the same conserved glycan species and relative proportion with the RMPs, the content of total afucosylated glycan in CT-P10 was slightly higher than in EU- or US-Rituximab.
29469653	5	49	theme	product	969:975	arg1	efficacy					977:984	product efficacy	969:984	product efficacy	969:984	In terms of the post-translational modification, CT-P10 contains slightly less N-terminal pyro-glutamate variant, which has been known not to affect product efficacy or safety.
29469653	6	50	theme	same	1071:1074	arg1	species					1093:1099	the same conserved glycan species	1067:1099	the same conserved glycan species	1067:1099	Oligosaccharide profiling has revealed that, although CT-P10 contains the same conserved glycan species and relative proportion with the RMPs, the content of total afucosylated glycan in CT-P10 was slightly higher than in EU- or US-Rituximab.
29469653	2	51	theme	biological	453:462	arg1	attributes					472:481	the physicochemical and biological quality attributes	429:481	the physicochemical and biological quality attributes	429:481	To demonstrate biosimilarity of CT-P10 with the reference medicinal product (RMP), extensive 3-way similarity assessment has been conducted between CT-P10, EU-Rituximab and US-Rituximab, focusing on the physicochemical and biological quality attributes.
29469653	0	52	theme	rituximab	36:44	arg1	assessment					22:31	Analytical similarity assessment	0:31	Analytical similarity assessment of rituximab	0:44	Analytical similarity assessment of rituximab biosimilar CT-P10 to reference medicinal product.
29469653	2	53	theme	physicochemical	433:447	arg1	attributes					472:481	the physicochemical and biological quality attributes	429:481	the physicochemical and biological quality attributes	429:481	To demonstrate biosimilarity of CT-P10 with the reference medicinal product (RMP), extensive 3-way similarity assessment has been conducted between CT-P10, EU-Rituximab and US-Rituximab, focusing on the physicochemical and biological quality attributes.
29469653	10	54	theme	clinical	1901:1908	arg1	results					1916:1922	clinical study results	1901:1922	clinical study results	1901:1922	The current 3-way similarity assessment together with clinical study results confidently demonstrate that CT-P10 is highly similar with EU- and US-Rituximab in terms of physicochemical properties, biological activities, efficacy, and safety for its final approval as a biosimilar product.
29469653	7	55	theme	afucosylation	1290:1302	arg1	level					1281:1285	the observed level	1268:1285	the observed level of afucosylation in CT-P10 drug product	1268:1325	Nevertheless, the effect of the observed level of afucosylation in CT-P10 drug product on Fc receptor binding affinity or antibody-dependent cell-mediated cytotoxicity was found to be negligible based on the spiking study with highly afucosylated sample.
29469653	10	56	theme	similarity	1865:1874	arg1	assessment					1876:1885	The current 3-way similarity assessment	1847:1885	The current 3-way similarity assessment together with clinical study results	1847:1922	The current 3-way similarity assessment together with clinical study results confidently demonstrate that CT-P10 is highly similar with EU- and US-Rituximab in terms of physicochemical properties, biological activities, efficacy, and safety for its final approval as a biosimilar product.
29469653	6	57	from	content	1144:1150	arg1	CT-P10					1184:1189	CT-P10	1184:1189	CT-P10	1184:1189	Oligosaccharide profiling has revealed that, although CT-P10 contains the same conserved glycan species and relative proportion with the RMPs, the content of total afucosylated glycan in CT-P10 was slightly higher than in EU- or US-Rituximab.
29469653	7	58	theme	level	1281:1285	arg1	effect					1258:1263	the effect	1254:1263	the effect of the observed level of afucosylation in CT-P10 drug product on Fc receptor binding affinity or antibody-dependent cell-mediated cytotoxicity	1254:1406	Nevertheless, the effect of the observed level of afucosylation in CT-P10 drug product on Fc receptor binding affinity or antibody-dependent cell-mediated cytotoxicity was found to be negligible based on the spiking study with highly afucosylated sample.
29469653	7	58	theme	level	1281:1285	arg1	negligible					1424:1433	negligible	1424:1433	negligible	1424:1433	Nevertheless, the effect of the observed level of afucosylation in CT-P10 drug product on Fc receptor binding affinity or antibody-dependent cell-mediated cytotoxicity was found to be negligible based on the spiking study with highly afucosylated sample.
29469653	10	59	theme	biosimilar	2116:2125	arg1	product					2127:2133	a biosimilar product	2114:2133	a biosimilar product	2114:2133	The current 3-way similarity assessment together with clinical study results confidently demonstrate that CT-P10 is highly similar with EU- and US-Rituximab in terms of physicochemical properties, biological activities, efficacy, and safety for its final approval as a biosimilar product.
29469653	8	60	theme	CT-P10	1636:1641	arg1	activities					1622:1631	biological activities	1611:1631	biological activities of CT-P10	1611:1641	Arrays of biological assays representative of known and putative mechanisms of action for rituximab have shown that biological activities of CT-P10 are within the quality range of RMPs.
29469653	3	61	theme	state-of-the-art	499:514	arg1	analyses					516:523	state-of-the-art analyses	499:523	state-of-the-art analyses	499:523	A multitude of state-of-the-art analyses revealed that CT-P10 has identical primary and higher order structures compared to the original product.
29469653	2	62	theme	3-way	323:327	arg1	assessment					340:349	extensive 3-way similarity assessment	313:349	extensive 3-way similarity assessment	313:349	To demonstrate biosimilarity of CT-P10 with the reference medicinal product (RMP), extensive 3-way similarity assessment has been conducted between CT-P10, EU-Rituximab and US-Rituximab, focusing on the physicochemical and biological quality attributes.
29469653	9	63	theme	Recent	1681:1686	arg1	results					1688:1694	Recent results	1681:1694	Recent results of clinical studies	1681:1714	Recent results of clinical studies have further confirmed that the CT-P10 exhibits equivalent clinical efficacy and safety profiles compared to EU- and US-Rituximab.
29469653	8	64	theme	quality	1658:1664	arg1	range					1666:1670	the quality range	1654:1670	the quality range of RMPs	1654:1678	Arrays of biological assays representative of known and putative mechanisms of action for rituximab have shown that biological activities of CT-P10 are within the quality range of RMPs.
29469653	10	65	with	similar	1970:1976	arg1	US-Rituximab					1991:2002	US-Rituximab	1991:2002	US-Rituximab	1991:2002	The current 3-way similarity assessment together with clinical study results confidently demonstrate that CT-P10 is highly similar with EU- and US-Rituximab in terms of physicochemical properties, biological activities, efficacy, and safety for its final approval as a biosimilar product.
29469653	10	65	with	similar	1970:1976	arg1	EU-					1983:1985	EU-	1983:1985	EU-	1983:1985	The current 3-way similarity assessment together with clinical study results confidently demonstrate that CT-P10 is highly similar with EU- and US-Rituximab in terms of physicochemical properties, biological activities, efficacy, and safety for its final approval as a biosimilar product.
29469653	1	66	theme	rituximab	157:165	arg1	product					178:184	the world's first rituximab biosimilar product	139:184	the world's first rituximab biosimilar product	139:184	CT-P10 (Truxima™) was recently approved as the world's first rituximab biosimilar product in the European Union (EU) and South Korea.
29469653	9	67	theme	studies	1708:1714	arg1	results					1688:1694	Recent results	1681:1694	Recent results of clinical studies	1681:1714	Recent results of clinical studies have further confirmed that the CT-P10 exhibits equivalent clinical efficacy and safety profiles compared to EU- and US-Rituximab.
29469653	7	68	theme	spiking	1448:1454	arg1	study					1456:1460	the spiking study	1444:1460	the spiking study with highly afucosylated sample	1444:1492	Nevertheless, the effect of the observed level of afucosylation in CT-P10 drug product on Fc receptor binding affinity or antibody-dependent cell-mediated cytotoxicity was found to be negligible based on the spiking study with highly afucosylated sample.
29469653	6	69	theme	glycan	1174:1179	arg1	content					1144:1150	the content	1140:1150	the content of total afucosylated glycan in CT-P10	1140:1189	Oligosaccharide profiling has revealed that, although CT-P10 contains the same conserved glycan species and relative proportion with the RMPs, the content of total afucosylated glycan in CT-P10 was slightly higher than in EU- or US-Rituximab.
29469653	6	69	theme	glycan	1174:1179	arg1	higher					1204:1209	higher	1204:1209	higher	1204:1209	Oligosaccharide profiling has revealed that, although CT-P10 contains the same conserved glycan species and relative proportion with the RMPs, the content of total afucosylated glycan in CT-P10 was slightly higher than in EU- or US-Rituximab.
29469653	10	70	theme	properties	2032:2041	arg1	terms					2007:2011	terms	2007:2011	terms of physicochemical properties, biological activities, efficacy, and safety for its final approval as a biosimilar product	2007:2133	The current 3-way similarity assessment together with clinical study results confidently demonstrate that CT-P10 is highly similar with EU- and US-Rituximab in terms of physicochemical properties, biological activities, efficacy, and safety for its final approval as a biosimilar product.
29469653	2	71	theme	reference	278:286	arg1	RMP					307:309	RMP	307:309	RMP	307:309	To demonstrate biosimilarity of CT-P10 with the reference medicinal product (RMP), extensive 3-way similarity assessment has been conducted between CT-P10, EU-Rituximab and US-Rituximab, focusing on the physicochemical and biological quality attributes.
29469653	2	71	theme	reference	278:286	arg1	product					298:304	the reference medicinal product	274:304	the reference medicinal product (RMP)	274:310	To demonstrate biosimilarity of CT-P10 with the reference medicinal product (RMP), extensive 3-way similarity assessment has been conducted between CT-P10, EU-Rituximab and US-Rituximab, focusing on the physicochemical and biological quality attributes.
29469653	3	72	theme	higher	572:577	arg1	structures					585:594	identical primary and higher order structures	550:594	identical primary and higher order structures	550:594	A multitude of state-of-the-art analyses revealed that CT-P10 has identical primary and higher order structures compared to the original product.
29469653	6	73	theme	total	1155:1159	arg1	glycan					1174:1179	total afucosylated glycan	1155:1179	total afucosylated glycan	1155:1179	Oligosaccharide profiling has revealed that, although CT-P10 contains the same conserved glycan species and relative proportion with the RMPs, the content of total afucosylated glycan in CT-P10 was slightly higher than in EU- or US-Rituximab.
29469653	4	74	theme	Purity/impurity	630:644	arg1	profiles					646:653	Purity/impurity profiles	630:653	Purity/impurity profiles of CT-P10 measured by the levels of aggregates, fragments, non-glycosylated form and process-related impurities	630:765	Purity/impurity profiles of CT-P10 measured by the levels of aggregates, fragments, non-glycosylated form and process-related impurities were also found to be comparable with those of RMPs.
29469653	1	75	from	Korea	223:227	arg1	approved					127:134	approved	127:134	approved	127:134	CT-P10 (Truxima™) was recently approved as the world's first rituximab biosimilar product in the European Union (EU) and South Korea.
29469653	10	76	theme	3-way	1859:1863	arg1	assessment					1876:1885	The current 3-way similarity assessment	1847:1885	The current 3-way similarity assessment together with clinical study results	1847:1922	The current 3-way similarity assessment together with clinical study results confidently demonstrate that CT-P10 is highly similar with EU- and US-Rituximab in terms of physicochemical properties, biological activities, efficacy, and safety for its final approval as a biosimilar product.
29469653	5	77	theme	modification	855:866	arg1	terms					823:827	terms	823:827	terms of the post-translational modification	823:866	In terms of the post-translational modification, CT-P10 contains slightly less N-terminal pyro-glutamate variant, which has been known not to affect product efficacy or safety.
29469653	3	78	theme	order	579:583	arg1	structures					585:594	identical primary and higher order structures	550:594	identical primary and higher order structures	550:594	A multitude of state-of-the-art analyses revealed that CT-P10 has identical primary and higher order structures compared to the original product.
29469653	9	79	theme	equivalent	1764:1773	arg1	efficacy					1784:1791	equivalent clinical efficacy	1764:1791	equivalent clinical efficacy	1764:1791	Recent results of clinical studies have further confirmed that the CT-P10 exhibits equivalent clinical efficacy and safety profiles compared to EU- and US-Rituximab.
29469653	10	80	theme	biological	2044:2053	arg1	properties					2032:2041	physicochemical properties	2016:2041	physicochemical properties	2016:2041	The current 3-way similarity assessment together with clinical study results confidently demonstrate that CT-P10 is highly similar with EU- and US-Rituximab in terms of physicochemical properties, biological activities, efficacy, and safety for its final approval as a biosimilar product.
29469653	10	80	theme	biological	2044:2053	arg1	activities					2055:2064	biological activities	2044:2064	biological activities	2044:2064	The current 3-way similarity assessment together with clinical study results confidently demonstrate that CT-P10 is highly similar with EU- and US-Rituximab in terms of physicochemical properties, biological activities, efficacy, and safety for its final approval as a biosimilar product.
29469653	3	81	theme	identical	550:558	arg1	structures					585:594	identical primary and higher order structures	550:594	identical primary and higher order structures	550:594	A multitude of state-of-the-art analyses revealed that CT-P10 has identical primary and higher order structures compared to the original product.
29469653	9	82	theme	efficacy	1784:1791	arg1	profiles					1804:1811	equivalent clinical efficacy and safety profiles	1764:1811	equivalent clinical efficacy and safety profiles	1764:1811	Recent results of clinical studies have further confirmed that the CT-P10 exhibits equivalent clinical efficacy and safety profiles compared to EU- and US-Rituximab.
29469653	8	83	theme	assays	1516:1521	arg1	Arrays					1495:1500	Arrays	1495:1500	Arrays of biological assays representative of known and putative mechanisms of action for rituximab	1495:1593	Arrays of biological assays representative of known and putative mechanisms of action for rituximab have shown that biological activities of CT-P10 are within the quality range of RMPs.
29469653	7	84	theme	cell-mediated	1381:1393	arg1	cytotoxicity					1395:1406	antibody-dependent cell-mediated cytotoxicity	1362:1406	antibody-dependent cell-mediated cytotoxicity	1362:1406	Nevertheless, the effect of the observed level of afucosylation in CT-P10 drug product on Fc receptor binding affinity or antibody-dependent cell-mediated cytotoxicity was found to be negligible based on the spiking study with highly afucosylated sample.
29469653	4	85	theme	form	731:734	arg1	levels					681:686	the levels	677:686	the levels of aggregates, fragments, non-glycosylated form and process-related impurities	677:765	Purity/impurity profiles of CT-P10 measured by the levels of aggregates, fragments, non-glycosylated form and process-related impurities were also found to be comparable with those of RMPs.
29469653	0	86	theme	medicinal	77:85	arg1	product					87:93	reference medicinal product	67:93	reference medicinal product	67:93	Analytical similarity assessment of rituximab biosimilar CT-P10 to reference medicinal product.
29469653	5	87	theme	N-terminal	899:908	arg1	variant					925:931	slightly less N-terminal pyro-glutamate variant	885:931	slightly less N-terminal pyro-glutamate variant	885:931	In terms of the post-translational modification, CT-P10 contains slightly less N-terminal pyro-glutamate variant, which has been known not to affect product efficacy or safety.
29469653	9	88	theme	safety	1797:1802	arg1	profiles					1804:1811	equivalent clinical efficacy and safety profiles	1764:1811	equivalent clinical efficacy and safety profiles	1764:1811	Recent results of clinical studies have further confirmed that the CT-P10 exhibits equivalent clinical efficacy and safety profiles compared to EU- and US-Rituximab.
29469653	8	89	theme	mechanisms	1560:1569	arg1	representative					1523:1536	representative	1523:1536	representative	1523:1536	Arrays of biological assays representative of known and putative mechanisms of action for rituximab have shown that biological activities of CT-P10 are within the quality range of RMPs.
29469653	2	90	theme	quality	464:470	arg1	attributes					472:481	the physicochemical and biological quality attributes	429:481	the physicochemical and biological quality attributes	429:481	To demonstrate biosimilarity of CT-P10 with the reference medicinal product (RMP), extensive 3-way similarity assessment has been conducted between CT-P10, EU-Rituximab and US-Rituximab, focusing on the physicochemical and biological quality attributes.
29469653	6	91	contain	contains	1058:1065	arg2	proportion					1114:1123	relative proportion	1105:1123	relative proportion with the RMPs	1105:1137	Oligosaccharide profiling has revealed that, although CT-P10 contains the same conserved glycan species and relative proportion with the RMPs, the content of total afucosylated glycan in CT-P10 was slightly higher than in EU- or US-Rituximab.
29469653	6	91	contain	contains	1058:1065	arg2	species					1093:1099	the same conserved glycan species	1067:1099	the same conserved glycan species	1067:1099	Oligosaccharide profiling has revealed that, although CT-P10 contains the same conserved glycan species and relative proportion with the RMPs, the content of total afucosylated glycan in CT-P10 was slightly higher than in EU- or US-Rituximab.
29469653	6	91	contain	contains	1058:1065	arg1	CT-P10					1051:1056	CT-P10	1051:1056	CT-P10	1051:1056	Oligosaccharide profiling has revealed that, although CT-P10 contains the same conserved glycan species and relative proportion with the RMPs, the content of total afucosylated glycan in CT-P10 was slightly higher than in EU- or US-Rituximab.
29469653	5	92	contain	contains	876:883	arg1	terms					823:827	terms	823:827	terms of the post-translational modification	823:866	In terms of the post-translational modification, CT-P10 contains slightly less N-terminal pyro-glutamate variant, which has been known not to affect product efficacy or safety.
29469653	5	92	contain	contains	876:883	arg2	CT-P10					869:874	CT-P10	869:874	CT-P10	869:874	In terms of the post-translational modification, CT-P10 contains slightly less N-terminal pyro-glutamate variant, which has been known not to affect product efficacy or safety.
29469653	5	92	contain	contains	876:883	arg1	CT-P10					869:874	CT-P10	869:874	CT-P10	869:874	In terms of the post-translational modification, CT-P10 contains slightly less N-terminal pyro-glutamate variant, which has been known not to affect product efficacy or safety.
29469653	5	92	contain	contains	876:883	arg2	variant					925:931	slightly less N-terminal pyro-glutamate variant	885:931	slightly less N-terminal pyro-glutamate variant	885:931	In terms of the post-translational modification, CT-P10 contains slightly less N-terminal pyro-glutamate variant, which has been known not to affect product efficacy or safety.
29469653	1	93	theme	European	193:200	arg1	EU					209:210	EU	209:210	EU	209:210	CT-P10 (Truxima™) was recently approved as the world's first rituximab biosimilar product in the European Union (EU) and South Korea.
29469653	1	93	theme	European	193:200	arg1	Union					202:206	the European Union	189:206	the European Union (EU)	189:211	CT-P10 (Truxima™) was recently approved as the world's first rituximab biosimilar product in the European Union (EU) and South Korea.
29469653	7	94	theme	receptor	1333:1340	arg1	affinity					1350:1357	Fc receptor binding affinity	1330:1357	Fc receptor binding affinity	1330:1357	Nevertheless, the effect of the observed level of afucosylation in CT-P10 drug product on Fc receptor binding affinity or antibody-dependent cell-mediated cytotoxicity was found to be negligible based on the spiking study with highly afucosylated sample.
29469653	8	95	theme	action	1574:1579	arg1	mechanisms					1560:1569	known and putative mechanisms	1541:1569	known and putative mechanisms of action for rituximab	1541:1593	Arrays of biological assays representative of known and putative mechanisms of action for rituximab have shown that biological activities of CT-P10 are within the quality range of RMPs.
29469653	0	96	theme	similarity	11:20	arg1	assessment					22:31	Analytical similarity assessment	0:31	Analytical similarity assessment of rituximab	0:44	Analytical similarity assessment of rituximab biosimilar CT-P10 to reference medicinal product.
29469653	4	97	theme	process-related	740:754	arg1	impurities					756:765	process-related impurities	740:765	process-related impurities	740:765	Purity/impurity profiles of CT-P10 measured by the levels of aggregates, fragments, non-glycosylated form and process-related impurities were also found to be comparable with those of RMPs.
29469653	6	98	theme	conserved	1076:1084	arg1	species					1093:1099	the same conserved glycan species	1067:1099	the same conserved glycan species	1067:1099	Oligosaccharide profiling has revealed that, although CT-P10 contains the same conserved glycan species and relative proportion with the RMPs, the content of total afucosylated glycan in CT-P10 was slightly higher than in EU- or US-Rituximab.
29469653	6	99	theme	relative	1105:1112	arg1	proportion					1114:1123	relative proportion	1105:1123	relative proportion with the RMPs	1105:1137	Oligosaccharide profiling has revealed that, although CT-P10 contains the same conserved glycan species and relative proportion with the RMPs, the content of total afucosylated glycan in CT-P10 was slightly higher than in EU- or US-Rituximab.
29469653	8	100	theme	biological	1611:1620	arg1	activities					1622:1631	biological activities	1611:1631	biological activities of CT-P10	1611:1641	Arrays of biological assays representative of known and putative mechanisms of action for rituximab have shown that biological activities of CT-P10 are within the quality range of RMPs.
29469653	10	101	theme	study	1910:1914	arg1	results					1916:1922	clinical study results	1901:1922	clinical study results	1901:1922	The current 3-way similarity assessment together with clinical study results confidently demonstrate that CT-P10 is highly similar with EU- and US-Rituximab in terms of physicochemical properties, biological activities, efficacy, and safety for its final approval as a biosimilar product.
29469653	7	102	with	study	1456:1460	arg1	sample					1487:1492	highly afucosylated sample	1467:1492	highly afucosylated sample	1467:1492	Nevertheless, the effect of the observed level of afucosylation in CT-P10 drug product on Fc receptor binding affinity or antibody-dependent cell-mediated cytotoxicity was found to be negligible based on the spiking study with highly afucosylated sample.
29469653	7	103	gly	afucosylation	1290:1302	arg1	product					1319:1325	CT-P10 drug product	1307:1325	CT-P10 drug product	1307:1325	Nevertheless, the effect of the observed level of afucosylation in CT-P10 drug product on Fc receptor binding affinity or antibody-dependent cell-mediated cytotoxicity was found to be negligible based on the spiking study with highly afucosylated sample.
29469653	3	104	contain	has	546:548	arg1	CT-P10					539:544	CT-P10	539:544	CT-P10	539:544	A multitude of state-of-the-art analyses revealed that CT-P10 has identical primary and higher order structures compared to the original product.
29469653	3	104	contain	has	546:548	arg2	structures					585:594	identical primary and higher order structures	550:594	identical primary and higher order structures	550:594	A multitude of state-of-the-art analyses revealed that CT-P10 has identical primary and higher order structures compared to the original product.
29469653	7	105	from	product	1319:1325	arg1	level					1281:1285	the observed level	1268:1285	the observed level of afucosylation in CT-P10 drug product	1268:1325	Nevertheless, the effect of the observed level of afucosylation in CT-P10 drug product on Fc receptor binding affinity or antibody-dependent cell-mediated cytotoxicity was found to be negligible based on the spiking study with highly afucosylated sample.
29469653	7	106	theme	observed	1272:1279	arg1	level					1281:1285	the observed level	1268:1285	the observed level of afucosylation in CT-P10 drug product	1268:1325	Nevertheless, the effect of the observed level of afucosylation in CT-P10 drug product on Fc receptor binding affinity or antibody-dependent cell-mediated cytotoxicity was found to be negligible based on the spiking study with highly afucosylated sample.
29469653	2	107	theme	similarity	329:338	arg1	assessment					340:349	extensive 3-way similarity assessment	313:349	extensive 3-way similarity assessment	313:349	To demonstrate biosimilarity of CT-P10 with the reference medicinal product (RMP), extensive 3-way similarity assessment has been conducted between CT-P10, EU-Rituximab and US-Rituximab, focusing on the physicochemical and biological quality attributes.
29469653	3	108	theme	analyses	516:523	arg1	multitude					486:494	A multitude	484:494	A multitude of state-of-the-art analyses	484:523	A multitude of state-of-the-art analyses revealed that CT-P10 has identical primary and higher order structures compared to the original product.
31853743	5	0	theme	solid	526:530	arg1	materials					542:550	solid, flexible materials	526:550	solid, flexible materials	526:550	These devices composed of solid, flexible materials do not absorb menstrual fluid and thus do not trap oxygen.
31853743	7	1	theme	shake	823:827	arg1	tests					738:742	three in vitro tests	723:742	three in vitro tests to evaluate devices for effect on TSST-1 production: (1) stationary flask, (2) shake flask, and (3) tampon sac	723:853	There are three in vitro tests to evaluate devices for effect on TSST-1 production: (1) stationary flask, (2) shake flask, and (3) tampon sac.
31853743	7	1	theme	shake	823:827	arg1	flask					829:833	(2) shake flask	819:833	(2) shake flask	819:833	There are three in vitro tests to evaluate devices for effect on TSST-1 production: (1) stationary flask, (2) shake flask, and (3) tampon sac.
31853743	8	2	theme	S.	1012:1013	arg1	growth					1022:1027	S. aureus growth	1012:1027	S. aureus growth	1012:1027	In this study, 100% rayon and 100% cotton tampons with three absorbencies, contraceptive diaphragms, and menstrual discs and cups were tested for effect on S. aureus growth and TSST-1 production.
31853743	9	3	theme	bacterial	1087:1095	arg1	growth					1097:1102	bacterial growth	1087:1102	bacterial growth	1087:1102	Product composition did not affect bacterial growth or TSST-1 production.
31853743	7	4	dep	flask	829:833	arg1	2					820:820	2	820:820	2	820:820	There are three in vitro tests to evaluate devices for effect on TSST-1 production: (1) stationary flask, (2) shake flask, and (3) tampon sac.
31853743	12	5	with	association	1596:1606	arg1	devices					1635:1641	non-absorbent devices	1621:1641	non-absorbent devices	1621:1641	These studies are consistent with the association of tampons with mTSS as a function of absorbency, but they suggest the occasional association of mTSS with non-absorbent devices may be coincidental as opposed to co-causative.
31853743	8	6	theme	100	886:888	arg1	%					889:889	%	889:889	%	889:889	In this study, 100% rayon and 100% cotton tampons with three absorbencies, contraceptive diaphragms, and menstrual discs and cups were tested for effect on S. aureus growth and TSST-1 production.
31853743	0	7	theme	superantigen	117:128	arg1	TSST-1					130:135	the superantigen TSST-1	113:135	the superantigen TSST-1	113:135	Effect of non-absorbent intravaginal menstrual/contraceptive products on Staphylococcus aureus and production of the superantigen TSST-1.
31853743	0	8	from	Effect	0:5	arg1	production					99:108	production	99:108	production of the superantigen TSST-1	99:135	Effect of non-absorbent intravaginal menstrual/contraceptive products on Staphylococcus aureus and production of the superantigen TSST-1.
31853743	0	8	from	Effect	0:5	arg1	aureus					88:93	Staphylococcus aureus	73:93	Staphylococcus aureus	73:93	Effect of non-absorbent intravaginal menstrual/contraceptive products on Staphylococcus aureus and production of the superantigen TSST-1.
31853743	10	9	theme	tampon	1310:1315	arg1	sacs					1317:1320	tampon sacs	1310:1320	tampon sacs	1310:1320	Tampons showed no effect on S. aureus growth compared with no-tampon controls, but tampons showed enhanced TSST-1 production as a function of trapped oxygen in stationary cultures and tampon sacs but not in shake flasks.
31853743	8	10	theme	rayon	876:880	arg1	tampons					898:904	100% rayon and 100% cotton tampons	871:904	100% rayon and 100% cotton tampons with three absorbencies, contraceptive diaphragms, and menstrual discs and cups	871:984	In this study, 100% rayon and 100% cotton tampons with three absorbencies, contraceptive diaphragms, and menstrual discs and cups were tested for effect on S. aureus growth and TSST-1 production.
31853743	6	11	theme	TSST-1	694:699	arg1	production					701:710	TSST-1 production	694:710	TSST-1 production	694:710	This study evaluates tampons and non-absorbent devices for effect on S. aureus and TSST-1 production.
31853743	7	12	dep	tests	738:742	arg1	tests					738:742	three in vitro tests	723:742	three in vitro tests to evaluate devices for effect on TSST-1 production: (1) stationary flask, (2) shake flask, and (3) tampon sac	723:853	There are three in vitro tests to evaluate devices for effect on TSST-1 production: (1) stationary flask, (2) shake flask, and (3) tampon sac.
31853743	7	12	dep	tests	738:742	arg1	sac					851:853	(3) tampon sac	840:853	(3) tampon sac	840:853	There are three in vitro tests to evaluate devices for effect on TSST-1 production: (1) stationary flask, (2) shake flask, and (3) tampon sac.
31853743	7	12	dep	tests	738:742	arg1	flask					812:816	(1) stationary flask	797:816	(1) stationary flask	797:816	There are three in vitro tests to evaluate devices for effect on TSST-1 production: (1) stationary flask, (2) shake flask, and (3) tampon sac.
31853743	7	12	dep	tests	738:742	arg1	flask					829:833	(2) shake flask	819:833	(2) shake flask	819:833	There are three in vitro tests to evaluate devices for effect on TSST-1 production: (1) stationary flask, (2) shake flask, and (3) tampon sac.
31853743	4	13	theme	medical	404:410	arg1	devices					412:418	medical devices	404:418	medical devices	404:418	There have been changes in use of medical devices to control menstrual flow, including increased use of menstrual discs and cups.
31853743	8	14	theme	100	871:873	arg1	%					874:874	%	874:874	%	874:874	In this study, 100% rayon and 100% cotton tampons with three absorbencies, contraceptive diaphragms, and menstrual discs and cups were tested for effect on S. aureus growth and TSST-1 production.
31853743	8	15	with	tampons	898:904	arg1	discs					971:975	menstrual discs	961:975	menstrual discs	961:975	In this study, 100% rayon and 100% cotton tampons with three absorbencies, contraceptive diaphragms, and menstrual discs and cups were tested for effect on S. aureus growth and TSST-1 production.
31853743	8	15	with	tampons	898:904	arg1	absorbencies					917:928	three absorbencies	911:928	three absorbencies	911:928	In this study, 100% rayon and 100% cotton tampons with three absorbencies, contraceptive diaphragms, and menstrual discs and cups were tested for effect on S. aureus growth and TSST-1 production.
31853743	8	15	with	tampons	898:904	arg1	diaphragms					945:954	contraceptive diaphragms	931:954	contraceptive diaphragms	931:954	In this study, 100% rayon and 100% cotton tampons with three absorbencies, contraceptive diaphragms, and menstrual discs and cups were tested for effect on S. aureus growth and TSST-1 production.
31853743	8	15	with	tampons	898:904	arg1	cups					981:984	cups	981:984	cups	981:984	In this study, 100% rayon and 100% cotton tampons with three absorbencies, contraceptive diaphragms, and menstrual discs and cups were tested for effect on S. aureus growth and TSST-1 production.
31853743	8	16	theme	contraceptive	931:943	arg1	diaphragms					945:954	contraceptive diaphragms	931:954	contraceptive diaphragms	931:954	In this study, 100% rayon and 100% cotton tampons with three absorbencies, contraceptive diaphragms, and menstrual discs and cups were tested for effect on S. aureus growth and TSST-1 production.
31853743	8	17	theme	%	874:874	arg1	rayon					876:880	100% rayon	871:880	100% rayon	871:880	In this study, 100% rayon and 100% cotton tampons with three absorbencies, contraceptive diaphragms, and menstrual discs and cups were tested for effect on S. aureus growth and TSST-1 production.
31853743	12	18	theme	mTSS	1611:1614	arg1	association					1596:1606	the occasional association	1581:1606	the occasional association of mTSS with non-absorbent devices	1581:1641	These studies are consistent with the association of tampons with mTSS as a function of absorbency, but they suggest the occasional association of mTSS with non-absorbent devices may be coincidental as opposed to co-causative.
31853743	0	19	theme	TSST-1	130:135	arg1	production					99:108	production	99:108	production of the superantigen TSST-1	99:135	Effect of non-absorbent intravaginal menstrual/contraceptive products on Staphylococcus aureus and production of the superantigen TSST-1.
31853743	4	20	theme	discs	484:488	arg1	use					467:469	increased use	457:469	increased use of menstrual discs and cups	457:497	There have been changes in use of medical devices to control menstrual flow, including increased use of menstrual discs and cups.
31853743	7	21	from	effect	768:773	arg1	production					785:794	TSST-1 production	778:794	TSST-1 production	778:794	There are three in vitro tests to evaluate devices for effect on TSST-1 production: (1) stationary flask, (2) shake flask, and (3) tampon sac.
31853743	7	22	theme	tampon	844:849	arg1	tests					738:742	three in vitro tests	723:742	three in vitro tests to evaluate devices for effect on TSST-1 production: (1) stationary flask, (2) shake flask, and (3) tampon sac	723:853	There are three in vitro tests to evaluate devices for effect on TSST-1 production: (1) stationary flask, (2) shake flask, and (3) tampon sac.
31853743	7	22	theme	tampon	844:849	arg1	sac					851:853	(3) tampon sac	840:853	(3) tampon sac	840:853	There are three in vitro tests to evaluate devices for effect on TSST-1 production: (1) stationary flask, (2) shake flask, and (3) tampon sac.
31853743	10	23	theme	S.	1154:1155	arg1	growth					1164:1169	S. aureus growth	1154:1169	S. aureus growth	1154:1169	Tampons showed no effect on S. aureus growth compared with no-tampon controls, but tampons showed enhanced TSST-1 production as a function of trapped oxygen in stationary cultures and tampon sacs but not in shake flasks.
31853743	10	24	theme	shake	1333:1337	arg1	flasks					1339:1344	shake flasks	1333:1344	shake flasks	1333:1344	Tampons showed no effect on S. aureus growth compared with no-tampon controls, but tampons showed enhanced TSST-1 production as a function of trapped oxygen in stationary cultures and tampon sacs but not in shake flasks.
31853743	4	25	theme	menstrual	474:482	arg1	discs					484:488	menstrual discs	474:488	menstrual discs	474:488	There have been changes in use of medical devices to control menstrual flow, including increased use of menstrual discs and cups.
31853743	10	26	theme	stationary	1286:1295	arg1	cultures					1297:1304	stationary cultures	1286:1304	stationary cultures	1286:1304	Tampons showed no effect on S. aureus growth compared with no-tampon controls, but tampons showed enhanced TSST-1 production as a function of trapped oxygen in stationary cultures and tampon sacs but not in shake flasks.
31853743	4	27	from	changes	386:392	arg1	use					397:399	use	397:399	use of medical devices to control menstrual flow	397:444	There have been changes in use of medical devices to control menstrual flow, including increased use of menstrual discs and cups.
31853743	7	28	theme	stationary	801:810	arg1	tests					738:742	three in vitro tests	723:742	three in vitro tests to evaluate devices for effect on TSST-1 production: (1) stationary flask, (2) shake flask, and (3) tampon sac	723:853	There are three in vitro tests to evaluate devices for effect on TSST-1 production: (1) stationary flask, (2) shake flask, and (3) tampon sac.
31853743	7	28	theme	stationary	801:810	arg1	flask					812:816	(1) stationary flask	797:816	(1) stationary flask	797:816	There are three in vitro tests to evaluate devices for effect on TSST-1 production: (1) stationary flask, (2) shake flask, and (3) tampon sac.
31853743	5	29	theme	menstrual	566:574	arg1	fluid					576:580	menstrual fluid	566:580	menstrual fluid	566:580	These devices composed of solid, flexible materials do not absorb menstrual fluid and thus do not trap oxygen.
31853743	11	30	theme	no-device	1444:1452	arg1	controls					1454:1461	no-device controls	1444:1461	no-device controls	1444:1461	The non-absorbent devices showed no enhanced S. aureus growth or TSST-1 production compared with no-device controls.
31853743	11	31	dep	S.	1392:1393	arg1	aureus					1395:1400	aureus	1395:1400	aureus	1395:1400	The non-absorbent devices showed no enhanced S. aureus growth or TSST-1 production compared with no-device controls.
31853743	8	32	theme	cotton	891:896	arg1	tampons					898:904	100% rayon and 100% cotton tampons	871:904	100% rayon and 100% cotton tampons with three absorbencies, contraceptive diaphragms, and menstrual discs and cups	871:984	In this study, 100% rayon and 100% cotton tampons with three absorbencies, contraceptive diaphragms, and menstrual discs and cups were tested for effect on S. aureus growth and TSST-1 production.
31853743	0	33	theme	intravaginal	24:35	arg1	products					61:68	non-absorbent intravaginal menstrual/contraceptive products	10:68	non-absorbent intravaginal menstrual/contraceptive products	10:68	Effect of non-absorbent intravaginal menstrual/contraceptive products on Staphylococcus aureus and production of the superantigen TSST-1.
31853743	7	34	theme	TSST-1	778:783	arg1	production					785:794	TSST-1 production	778:794	TSST-1 production	778:794	There are three in vitro tests to evaluate devices for effect on TSST-1 production: (1) stationary flask, (2) shake flask, and (3) tampon sac.
31853743	8	35	theme	menstrual	961:969	arg1	discs					971:975	menstrual discs	961:975	menstrual discs	961:975	In this study, 100% rayon and 100% cotton tampons with three absorbencies, contraceptive diaphragms, and menstrual discs and cups were tested for effect on S. aureus growth and TSST-1 production.
31853743	8	36	theme	%	889:889	arg1	cotton					891:896	100% cotton	886:896	100% cotton	886:896	In this study, 100% rayon and 100% cotton tampons with three absorbencies, contraceptive diaphragms, and menstrual discs and cups were tested for effect on S. aureus growth and TSST-1 production.
31853743	6	37	from	effect	670:675	arg1	aureus					683:688	S. aureus	680:688	S. aureus	680:688	This study evaluates tampons and non-absorbent devices for effect on S. aureus and TSST-1 production.
31853743	6	37	from	effect	670:675	arg1	production					701:710	TSST-1 production	694:710	TSST-1 production	694:710	This study evaluates tampons and non-absorbent devices for effect on S. aureus and TSST-1 production.
31853743	5	38	dep	solid	526:530	arg1	flexible					533:540	flexible	533:540	flexible	533:540	These devices composed of solid, flexible materials do not absorb menstrual fluid and thus do not trap oxygen.
31853743	10	39	theme	enhanced	1224:1231	arg1	production					1240:1249	enhanced TSST-1 production	1224:1249	enhanced TSST-1 production	1224:1249	Tampons showed no effect on S. aureus growth compared with no-tampon controls, but tampons showed enhanced TSST-1 production as a function of trapped oxygen in stationary cultures and tampon sacs but not in shake flasks.
31853743	10	39	theme	enhanced	1224:1231	arg1	function					1256:1263	a function	1254:1263	a function of trapped oxygen	1254:1281	Tampons showed no effect on S. aureus growth compared with no-tampon controls, but tampons showed enhanced TSST-1 production as a function of trapped oxygen in stationary cultures and tampon sacs but not in shake flasks.
31853743	0	40	theme	non-absorbent	10:22	arg1	products					61:68	non-absorbent intravaginal menstrual/contraceptive products	10:68	non-absorbent intravaginal menstrual/contraceptive products	10:68	Effect of non-absorbent intravaginal menstrual/contraceptive products on Staphylococcus aureus and production of the superantigen TSST-1.
31853743	11	41	theme	S.	1392:1393	arg1	growth					1402:1407	no enhanced S. aureus growth or TSST-1 production	1380:1428	growth	1402:1407	The non-absorbent devices showed no enhanced S. aureus growth or TSST-1 production compared with no-device controls.
31853743	10	42	theme	trapped	1268:1274	arg1	oxygen					1276:1281	trapped oxygen	1268:1281	trapped oxygen	1268:1281	Tampons showed no effect on S. aureus growth compared with no-tampon controls, but tampons showed enhanced TSST-1 production as a function of trapped oxygen in stationary cultures and tampon sacs but not in shake flasks.
31853743	12	43	theme	tampons	1517:1523	arg1	association					1502:1512	the association	1498:1512	the association of tampons with mTSS	1498:1533	These studies are consistent with the association of tampons with mTSS as a function of absorbency, but they suggest the occasional association of mTSS with non-absorbent devices may be coincidental as opposed to co-causative.
31853743	0	44	theme	products	61:68	arg1	Effect					0:5	Effect	0:5	Effect of non-absorbent intravaginal menstrual/contraceptive products on Staphylococcus aureus and production of the superantigen TSST-1.	0:136	Effect of non-absorbent intravaginal menstrual/contraceptive products on Staphylococcus aureus and production of the superantigen TSST-1.
31853743	9	45	theme	TSST-1	1107:1112	arg1	production					1114:1123	TSST-1 production	1107:1123	TSST-1 production	1107:1123	Product composition did not affect bacterial growth or TSST-1 production.
31853743	6	46	theme	non-absorbent	644:656	arg1	devices					658:664	non-absorbent devices	644:664	non-absorbent devices	644:664	This study evaluates tampons and non-absorbent devices for effect on S. aureus and TSST-1 production.
31853743	11	47	theme	TSST-1	1412:1417	arg1	production					1419:1428	no enhanced S. aureus growth or TSST-1 production	1380:1428	production	1419:1428	The non-absorbent devices showed no enhanced S. aureus growth or TSST-1 production compared with no-device controls.
31853743	0	48	theme	menstrual/contraceptive	37:59	arg1	products					61:68	non-absorbent intravaginal menstrual/contraceptive products	10:68	non-absorbent intravaginal menstrual/contraceptive products	10:68	Effect of non-absorbent intravaginal menstrual/contraceptive products on Staphylococcus aureus and production of the superantigen TSST-1.
31853743	4	49	theme	increased	457:465	arg1	use					467:469	increased use	457:469	increased use of menstrual discs and cups	457:497	There have been changes in use of medical devices to control menstrual flow, including increased use of menstrual discs and cups.
31853743	8	50	theme	TSST-1	1033:1038	arg1	production					1040:1049	TSST-1 production	1033:1049	TSST-1 production	1033:1049	In this study, 100% rayon and 100% cotton tampons with three absorbencies, contraceptive diaphragms, and menstrual discs and cups were tested for effect on S. aureus growth and TSST-1 production.
31853743	8	51	from	effect	1002:1007	arg1	growth					1022:1027	S. aureus growth	1012:1027	S. aureus growth	1012:1027	In this study, 100% rayon and 100% cotton tampons with three absorbencies, contraceptive diaphragms, and menstrual discs and cups were tested for effect on S. aureus growth and TSST-1 production.
31853743	8	51	from	effect	1002:1007	arg1	production					1040:1049	TSST-1 production	1033:1049	TSST-1 production	1033:1049	In this study, 100% rayon and 100% cotton tampons with three absorbencies, contraceptive diaphragms, and menstrual discs and cups were tested for effect on S. aureus growth and TSST-1 production.
31853743	2	52	theme	oxygen	230:235	arg1	introduction					237:248	oxygen introduction	230:248	oxygen introduction within tampons into the anaerobic vagina	230:289	One reason for this association is oxygen introduction within tampons into the anaerobic vagina.
31853743	12	53	with	consistent	1482:1491	arg1	association					1502:1512	the association	1498:1512	the association of tampons with mTSS	1498:1533	These studies are consistent with the association of tampons with mTSS as a function of absorbency, but they suggest the occasional association of mTSS with non-absorbent devices may be coincidental as opposed to co-causative.
31853743	8	54	dep	S.	1012:1013	arg1	aureus					1015:1020	aureus	1015:1020	aureus	1015:1020	In this study, 100% rayon and 100% cotton tampons with three absorbencies, contraceptive diaphragms, and menstrual discs and cups were tested for effect on S. aureus growth and TSST-1 production.
31853743	7	55	dep	flask	812:816	arg1	1					798:798	1	798:798	1	798:798	There are three in vitro tests to evaluate devices for effect on TSST-1 production: (1) stationary flask, (2) shake flask, and (3) tampon sac.
31853743	7	56	dep	in	729:730	arg1	vitro					732:736	vitro	732:736	vitro	732:736	There are three in vitro tests to evaluate devices for effect on TSST-1 production: (1) stationary flask, (2) shake flask, and (3) tampon sac.
31853743	11	57	theme	non-absorbent	1351:1363	arg1	devices					1365:1371	The non-absorbent devices	1347:1371	The non-absorbent devices	1347:1371	The non-absorbent devices showed no enhanced S. aureus growth or TSST-1 production compared with no-device controls.
31853743	10	58	theme	oxygen	1276:1281	arg1	production					1240:1249	enhanced TSST-1 production	1224:1249	enhanced TSST-1 production	1224:1249	Tampons showed no effect on S. aureus growth compared with no-tampon controls, but tampons showed enhanced TSST-1 production as a function of trapped oxygen in stationary cultures and tampon sacs but not in shake flasks.
31853743	10	58	theme	oxygen	1276:1281	arg1	function					1256:1263	a function	1254:1263	a function of trapped oxygen	1254:1281	Tampons showed no effect on S. aureus growth compared with no-tampon controls, but tampons showed enhanced TSST-1 production as a function of trapped oxygen in stationary cultures and tampon sacs but not in shake flasks.
31853743	4	59	theme	devices	412:418	arg1	use					397:399	use	397:399	use of medical devices to control menstrual flow	397:444	There have been changes in use of medical devices to control menstrual flow, including increased use of menstrual discs and cups.
31853743	2	60	theme	anaerobic	274:282	arg1	vagina					284:289	the anaerobic vagina	270:289	the anaerobic vagina	270:289	One reason for this association is oxygen introduction within tampons into the anaerobic vagina.
31853743	9	61	theme	Product	1052:1058	arg1	composition					1060:1070	Product composition	1052:1070	Product composition	1052:1070	Product composition did not affect bacterial growth or TSST-1 production.
31853743	7	62	theme	in	729:730	arg1	tests					738:742	three in vitro tests	723:742	three in vitro tests to evaluate devices for effect on TSST-1 production: (1) stationary flask, (2) shake flask, and (3) tampon sac	723:853	There are three in vitro tests to evaluate devices for effect on TSST-1 production: (1) stationary flask, (2) shake flask, and (3) tampon sac.
31853743	7	62	theme	in	729:730	arg1	sac					851:853	(3) tampon sac	840:853	(3) tampon sac	840:853	There are three in vitro tests to evaluate devices for effect on TSST-1 production: (1) stationary flask, (2) shake flask, and (3) tampon sac.
31853743	7	62	theme	in	729:730	arg1	flask					812:816	(1) stationary flask	797:816	(1) stationary flask	797:816	There are three in vitro tests to evaluate devices for effect on TSST-1 production: (1) stationary flask, (2) shake flask, and (3) tampon sac.
31853743	7	62	theme	in	729:730	arg1	flask					829:833	(2) shake flask	819:833	(2) shake flask	819:833	There are three in vitro tests to evaluate devices for effect on TSST-1 production: (1) stationary flask, (2) shake flask, and (3) tampon sac.
31853743	4	63	theme	menstrual	431:439	arg1	flow					441:444	menstrual flow	431:444	menstrual flow	431:444	There have been changes in use of medical devices to control menstrual flow, including increased use of menstrual discs and cups.
31853743	10	64	dep	S.	1154:1155	arg1	aureus					1157:1162	aureus	1157:1162	aureus	1157:1162	Tampons showed no effect on S. aureus growth compared with no-tampon controls, but tampons showed enhanced TSST-1 production as a function of trapped oxygen in stationary cultures and tampon sacs but not in shake flasks.
31853743	10	65	theme	no-tampon	1185:1193	arg1	controls					1195:1202	no-tampon controls	1185:1202	no-tampon controls	1185:1202	Tampons showed no effect on S. aureus growth compared with no-tampon controls, but tampons showed enhanced TSST-1 production as a function of trapped oxygen in stationary cultures and tampon sacs but not in shake flasks.
31853743	12	66	theme	occasional	1585:1594	arg1	association					1596:1606	the occasional association	1581:1606	the occasional association of mTSS with non-absorbent devices	1581:1641	These studies are consistent with the association of tampons with mTSS as a function of absorbency, but they suggest the occasional association of mTSS with non-absorbent devices may be coincidental as opposed to co-causative.
31853743	3	67	theme	TSS	348:350	arg1	TSST-1					361:366	TSST-1	361:366	TSST-1	361:366	Oxygen is required for Staphylococcus aureus to produce TSS toxin-1 (TSST-1).
31853743	3	67	theme	TSS	348:350	arg1	toxin-1					352:358	TSS toxin-1	348:358	TSS toxin-1 (TSST-1)	348:367	Oxygen is required for Staphylococcus aureus to produce TSS toxin-1 (TSST-1).
31853743	7	68	dep	sac	851:853	arg1	3					841:841	3	841:841	3	841:841	There are three in vitro tests to evaluate devices for effect on TSST-1 production: (1) stationary flask, (2) shake flask, and (3) tampon sac.
31853743	12	69	theme	non-absorbent	1621:1633	arg1	devices					1635:1641	non-absorbent devices	1621:1641	non-absorbent devices	1621:1641	These studies are consistent with the association of tampons with mTSS as a function of absorbency, but they suggest the occasional association of mTSS with non-absorbent devices may be coincidental as opposed to co-causative.
31853743	12	70	theme	absorbency	1552:1561	arg1	function					1540:1547	a function	1538:1547	a function of absorbency	1538:1561	These studies are consistent with the association of tampons with mTSS as a function of absorbency, but they suggest the occasional association of mTSS with non-absorbent devices may be coincidental as opposed to co-causative.
31853743	1	71	theme	toxic	166:170	arg1	mTSS					188:191	mTSS	188:191	mTSS	188:191	Tampons are associated with toxic shock syndrome (mTSS).
31853743	1	71	theme	toxic	166:170	arg1	syndrome					178:185	toxic shock syndrome	166:185	toxic shock syndrome (mTSS)	166:192	Tampons are associated with toxic shock syndrome (mTSS).
31853743	12	72	dep	suggest	1573:1579	arg1	coincidental					1650:1661	coincidental	1650:1661	coincidental	1650:1661	These studies are consistent with the association of tampons with mTSS as a function of absorbency, but they suggest the occasional association of mTSS with non-absorbent devices may be coincidental as opposed to co-causative.
31853743	4	73	theme	cups	494:497	arg1	use					467:469	increased use	457:469	increased use of menstrual discs and cups	457:497	There have been changes in use of medical devices to control menstrual flow, including increased use of menstrual discs and cups.
31853743	12	74	with	association	1502:1512	arg1	mTSS					1530:1533	mTSS	1530:1533	mTSS	1530:1533	These studies are consistent with the association of tampons with mTSS as a function of absorbency, but they suggest the occasional association of mTSS with non-absorbent devices may be coincidental as opposed to co-causative.
31853743	10	75	theme	TSST-1	1233:1238	arg1	production					1240:1249	enhanced TSST-1 production	1224:1249	enhanced TSST-1 production	1224:1249	Tampons showed no effect on S. aureus growth compared with no-tampon controls, but tampons showed enhanced TSST-1 production as a function of trapped oxygen in stationary cultures and tampon sacs but not in shake flasks.
31853743	10	75	theme	TSST-1	1233:1238	arg1	function					1256:1263	a function	1254:1263	a function of trapped oxygen	1254:1281	Tampons showed no effect on S. aureus growth compared with no-tampon controls, but tampons showed enhanced TSST-1 production as a function of trapped oxygen in stationary cultures and tampon sacs but not in shake flasks.
31853743	1	76	theme	shock	172:176	arg1	mTSS					188:191	mTSS	188:191	mTSS	188:191	Tampons are associated with toxic shock syndrome (mTSS).
31853743	1	76	theme	shock	172:176	arg1	syndrome					178:185	toxic shock syndrome	166:185	toxic shock syndrome (mTSS)	166:192	Tampons are associated with toxic shock syndrome (mTSS).
31853743	11	77	theme	enhanced	1383:1390	arg1	growth					1402:1407	no enhanced S. aureus growth or TSST-1 production	1380:1428	growth	1402:1407	The non-absorbent devices showed no enhanced S. aureus growth or TSST-1 production compared with no-device controls.
31135332	4	0	theme	gene	303:306	arg1	similarity					317:326	16S rRNA gene sequence similarity	294:326	16S rRNA gene sequence similarity	294:326	On the basis of 16S rRNA gene sequence similarity, strain KGMB04489T belonged to the genus Olsenella and was most closely related to Olsenella scatoligenes SK9K4T (94.3 %), Olsenella uli ATCC 49627T (93.5 %), Olsenella umbonata lac31T (93.4 %) and Olsenella profusa D315A-29T (93.3 %).
31135332	10	1	theme	phylogenetic	957:968	arg1	characteristics					1004:1018	phylogenetic, physiological and chemotaxonomic characteristics	957:1018	phylogenetic, physiological and chemotaxonomic characteristics	957:1018	Based on phylogenetic, physiological and chemotaxonomic characteristics, strain KGMB04489T is considered to represent a novel species within the genus Olsenella, for which the name Olsenellafaecalis sp.
31135332	5	2	theme	major	568:572	arg1	acid					597:600	lactic acid	590:600	lactic acid	590:600	The major end product was lactic acid.
31135332	5	2	theme	major	568:572	arg1	product					578:584	The major end product	564:584	The major end product	564:584	The major end product was lactic acid.
31135332	2	3	theme	actinobacterial	94:108	arg1	strain					110:115	A novel actinobacterial strain	86:115	A novel actinobacterial strain	86:115	A novel actinobacterial strain, designated KGMB04489T, was isolated from the faeces of a healthy Korean.
31135332	5	4	theme	end	574:576	arg1	acid					597:600	lactic acid	590:600	lactic acid	590:600	The major end product was lactic acid.
31135332	5	4	theme	end	574:576	arg1	product					578:584	The major end product	564:584	The major end product	564:584	The major end product was lactic acid.
31135332	6	5	theme	65.5 mol	627:634	arg1	G+C content					611:621	The DNA G+C content	603:621	The DNA G+C content	603:621	The DNA G+C content was 65.5 mol%.
31135332	6	5	theme	65.5 mol	627:634	arg1	%					635:635	65.5 mol%	627:635	65.5 mol%	627:635	The DNA G+C content was 65.5 mol%.
31135332	8	6	theme	Strain	737:742	arg1	KGMB04489T					744:753	Strain KGMB04489T	737:753	Strain KGMB04489T	737:753	Strain KGMB04489T contained meso-diaminopimelic acid as the diamino acid in the peptidoglycan.
31135332	8	7	contain	contained	755:763	arg1	KGMB04489T					744:753	Strain KGMB04489T	737:753	Strain KGMB04489T	737:753	Strain KGMB04489T contained meso-diaminopimelic acid as the diamino acid in the peptidoglycan.
31135332	8	7	contain	contained	755:763	arg2	acid					785:788	meso-diaminopimelic acid	765:788	meso-diaminopimelic acid	765:788	Strain KGMB04489T contained meso-diaminopimelic acid as the diamino acid in the peptidoglycan.
31135332	8	7	contain	contained	755:763	arg2	acid					805:808	the diamino acid	793:808	the diamino acid in the peptidoglycan	793:829	Strain KGMB04489T contained meso-diaminopimelic acid as the diamino acid in the peptidoglycan.
31135332	10	8	theme	novel	1068:1072	arg1	species					1074:1080	a novel species	1066:1080	a novel species	1066:1080	Based on phylogenetic, physiological and chemotaxonomic characteristics, strain KGMB04489T is considered to represent a novel species within the genus Olsenella, for which the name Olsenellafaecalis sp.
31135332	12	9	theme	=KCTC	1200:1204	arg1	KGMB04489T					1188:1197	KGMB04489T	1188:1197	KGMB04489T (=KCTC 15699T=CCUG 72345T)	1188:1224	The type strain is KGMB04489T (=KCTC 15699T=CCUG 72345T).
31135332	12	9	theme	=KCTC	1200:1204	arg1	72345T					1218:1223	=KCTC 15699T=CCUG 72345T	1200:1223	=KCTC 15699T=CCUG 72345T	1200:1223	The type strain is KGMB04489T (=KCTC 15699T=CCUG 72345T).
31135332	9	10	theme	polar	836:840	arg1	lipids					842:847	The polar lipids	832:847	The polar lipids	832:847	The polar lipids consisted of an unidentified phospholipid, six unidentified glycolipids and an unidentified lipid.
31135332	10	11	theme	chemotaxonomic	989:1002	arg1	characteristics					1004:1018	phylogenetic, physiological and chemotaxonomic characteristics	957:1018	phylogenetic, physiological and chemotaxonomic characteristics	957:1018	Based on phylogenetic, physiological and chemotaxonomic characteristics, strain KGMB04489T is considered to represent a novel species within the genus Olsenella, for which the name Olsenellafaecalis sp.
31135332	1	12	theme	human	72:76	arg1	faeces					78:83	human faeces	72:83	human faeces	72:83	nov., an anaerobic actinobacterium isolated from human faeces.
31135332	6	13	theme	DNA	607:609	arg1	G+C content					611:621	The DNA G+C content	603:621	The DNA G+C content	603:621	The DNA G+C content was 65.5 mol%.
31135332	6	13	theme	DNA	607:609	arg1	%					635:635	65.5 mol%	627:635	65.5 mol%	627:635	The DNA G+C content was 65.5 mol%.
31135332	0	14	theme	faecalis	10:17	arg1	sp					19:20	Olsenella faecalis sp	0:20	Olsenella faecalis sp.	0:21	Olsenella faecalis sp.
31135332	8	15	from	acid	805:808	arg1	peptidoglycan					817:829	the peptidoglycan	813:829	the peptidoglycan	813:829	Strain KGMB04489T contained meso-diaminopimelic acid as the diamino acid in the peptidoglycan.
31135332	0	16	theme	Olsenella	0:8	arg1	sp					19:20	Olsenella faecalis sp	0:20	Olsenella faecalis sp.	0:21	Olsenella faecalis sp.
31135332	4	17	theme	genus	363:367	arg1	Olsenella					369:377	the genus Olsenella	359:377	the genus Olsenella	359:377	On the basis of 16S rRNA gene sequence similarity, strain KGMB04489T belonged to the genus Olsenella and was most closely related to Olsenella scatoligenes SK9K4T (94.3 %), Olsenella uli ATCC 49627T (93.5 %), Olsenella umbonata lac31T (93.4 %) and Olsenella profusa D315A-29T (93.3 %).
31135332	7	18	theme	 1cis9	713:718	arg1	DMA					720:722	 1cis9 DMA	713:722	 1cis9 DMA	713:722	The major cellular fatty acids of strain KGMB04489T were C18 : 1cis9, C18 : 1cis9 DMA and C16 : 0.
31135332	3	19	theme	strain	204:209	arg1	Cells					191:195	Cells	191:195	Cells of the strain	191:209	Cells of the strain were strictly anaerobic, Gram-stain-positive and short-rod-shaped.
31135332	8	20	theme	meso-diaminopimelic	765:783	arg1	acid					785:788	meso-diaminopimelic acid	765:788	meso-diaminopimelic acid	765:788	Strain KGMB04489T contained meso-diaminopimelic acid as the diamino acid in the peptidoglycan.
31135332	8	20	theme	meso-diaminopimelic	765:783	arg1	acid					805:808	the diamino acid	793:808	the diamino acid in the peptidoglycan	793:829	Strain KGMB04489T contained meso-diaminopimelic acid as the diamino acid in the peptidoglycan.
31135332	9	21	theme	unidentified	865:876	arg1	phospholipid					878:889	an unidentified phospholipid	862:889	an unidentified phospholipid	862:889	The polar lipids consisted of an unidentified phospholipid, six unidentified glycolipids and an unidentified lipid.
31135332	10	22	theme	strain	1021:1026	arg1	KGMB04489T					1028:1037	strain KGMB04489T	1021:1037	strain KGMB04489T	1021:1037	Based on phylogenetic, physiological and chemotaxonomic characteristics, strain KGMB04489T is considered to represent a novel species within the genus Olsenella, for which the name Olsenellafaecalis sp.
31135332	4	23	dep	similarity	317:326	arg1	the					281:283	the	281:283	the	281:283	On the basis of 16S rRNA gene sequence similarity, strain KGMB04489T belonged to the genus Olsenella and was most closely related to Olsenella scatoligenes SK9K4T (94.3 %), Olsenella uli ATCC 49627T (93.5 %), Olsenella umbonata lac31T (93.4 %) and Olsenella profusa D315A-29T (93.3 %).
31135332	4	23	dep	similarity	317:326	arg1	basis					285:289	basis	285:289	basis	285:289	On the basis of 16S rRNA gene sequence similarity, strain KGMB04489T belonged to the genus Olsenella and was most closely related to Olsenella scatoligenes SK9K4T (94.3 %), Olsenella uli ATCC 49627T (93.5 %), Olsenella umbonata lac31T (93.4 %) and Olsenella profusa D315A-29T (93.3 %).
31135332	2	24	theme	novel	88:92	arg1	strain					110:115	A novel actinobacterial strain	86:115	A novel actinobacterial strain	86:115	A novel actinobacterial strain, designated KGMB04489T, was isolated from the faeces of a healthy Korean.
31135332	7	25	theme	major	642:646	arg1	C18 					695:698	C18 	695:698	C18 	695:698	The major cellular fatty acids of strain KGMB04489T were C18 : 1cis9, C18 : 1cis9 DMA and C16 : 0.
31135332	7	25	theme	major	642:646	arg1	acids					663:667	The major cellular fatty acids	638:667	The major cellular fatty acids of strain KGMB04489T	638:688	The major cellular fatty acids of strain KGMB04489T were C18 : 1cis9, C18 : 1cis9 DMA and C16 : 0.
31135332	12	26	theme	type	1173:1176	arg1	KGMB04489T					1188:1197	KGMB04489T	1188:1197	KGMB04489T (=KCTC 15699T=CCUG 72345T)	1188:1224	The type strain is KGMB04489T (=KCTC 15699T=CCUG 72345T).
31135332	12	26	theme	type	1173:1176	arg1	strain					1178:1183	The type strain	1169:1183	The type strain	1169:1183	The type strain is KGMB04489T (=KCTC 15699T=CCUG 72345T).
31135332	7	27	theme	KGMB04489T	679:688	arg1	C18 					695:698	C18 	695:698	C18 	695:698	The major cellular fatty acids of strain KGMB04489T were C18 : 1cis9, C18 : 1cis9 DMA and C16 : 0.
31135332	7	27	theme	KGMB04489T	679:688	arg1	acids					663:667	The major cellular fatty acids	638:667	The major cellular fatty acids of strain KGMB04489T	638:688	The major cellular fatty acids of strain KGMB04489T were C18 : 1cis9, C18 : 1cis9 DMA and C16 : 0.
31135332	8	28	theme	diamino	797:803	arg1	acid					785:788	meso-diaminopimelic acid	765:788	meso-diaminopimelic acid	765:788	Strain KGMB04489T contained meso-diaminopimelic acid as the diamino acid in the peptidoglycan.
31135332	8	28	theme	diamino	797:803	arg1	acid					805:808	the diamino acid	793:808	the diamino acid in the peptidoglycan	793:829	Strain KGMB04489T contained meso-diaminopimelic acid as the diamino acid in the peptidoglycan.
31135332	5	29	theme	lactic	590:595	arg1	acid					597:600	lactic acid	590:600	lactic acid	590:600	The major end product was lactic acid.
31135332	5	29	theme	lactic	590:595	arg1	product					578:584	The major end product	564:584	The major end product	564:584	The major end product was lactic acid.
31135332	2	30	attach	isolated	145:152	arg1	faeces					163:168	the faeces	159:168	the faeces of a healthy Korean	159:188	A novel actinobacterial strain, designated KGMB04489T, was isolated from the faeces of a healthy Korean.
31135332	2	30	attach	isolated	145:152	arg2	strain					110:115	A novel actinobacterial strain	86:115	A novel actinobacterial strain	86:115	A novel actinobacterial strain, designated KGMB04489T, was isolated from the faeces of a healthy Korean.
31135332	9	31	theme	unidentified	896:907	arg1	glycolipids					909:919	six unidentified glycolipids	892:919	six unidentified glycolipids	892:919	The polar lipids consisted of an unidentified phospholipid, six unidentified glycolipids and an unidentified lipid.
31135332	7	32	theme	strain	672:677	arg1	KGMB04489T					679:688	strain KGMB04489T	672:688	strain KGMB04489T	672:688	The major cellular fatty acids of strain KGMB04489T were C18 : 1cis9, C18 : 1cis9 DMA and C16 : 0.
31135332	2	33	theme	Korean	183:188	arg1	faeces					163:168	the faeces	159:168	the faeces of a healthy Korean	159:188	A novel actinobacterial strain, designated KGMB04489T, was isolated from the faeces of a healthy Korean.
31135332	4	34	theme	strain	329:334	arg1	KGMB04489T					336:345	strain KGMB04489T	329:345	strain KGMB04489T	329:345	On the basis of 16S rRNA gene sequence similarity, strain KGMB04489T belonged to the genus Olsenella and was most closely related to Olsenella scatoligenes SK9K4T (94.3 %), Olsenella uli ATCC 49627T (93.5 %), Olsenella umbonata lac31T (93.4 %) and Olsenella profusa D315A-29T (93.3 %).
31135332	10	35	theme	Olsenellafaecalis	1129:1145	arg1	sp					1147:1148	the name Olsenellafaecalis sp	1120:1148	the name Olsenellafaecalis sp	1120:1148	Based on phylogenetic, physiological and chemotaxonomic characteristics, strain KGMB04489T is considered to represent a novel species within the genus Olsenella, for which the name Olsenellafaecalis sp.
31135332	7	36	theme	fatty	657:661	arg1	C18 					695:698	C18 	695:698	C18 	695:698	The major cellular fatty acids of strain KGMB04489T were C18 : 1cis9, C18 : 1cis9 DMA and C16 : 0.
31135332	7	36	theme	fatty	657:661	arg1	acids					663:667	The major cellular fatty acids	638:667	The major cellular fatty acids of strain KGMB04489T	638:688	The major cellular fatty acids of strain KGMB04489T were C18 : 1cis9, C18 : 1cis9 DMA and C16 : 0.
31135332	2	37	theme	healthy	175:181	arg1	Korean					183:188	a healthy Korean	173:188	a healthy Korean	173:188	A novel actinobacterial strain, designated KGMB04489T, was isolated from the faeces of a healthy Korean.
31135332	12	38	theme	15699T=CCUG	1206:1216	arg1	KGMB04489T					1188:1197	KGMB04489T	1188:1197	KGMB04489T (=KCTC 15699T=CCUG 72345T)	1188:1224	The type strain is KGMB04489T (=KCTC 15699T=CCUG 72345T).
31135332	12	38	theme	15699T=CCUG	1206:1216	arg1	72345T					1218:1223	=KCTC 15699T=CCUG 72345T	1200:1223	=KCTC 15699T=CCUG 72345T	1200:1223	The type strain is KGMB04489T (=KCTC 15699T=CCUG 72345T).
31135332	4	39	theme	rRNA	298:301	arg1	similarity					317:326	16S rRNA gene sequence similarity	294:326	16S rRNA gene sequence similarity	294:326	On the basis of 16S rRNA gene sequence similarity, strain KGMB04489T belonged to the genus Olsenella and was most closely related to Olsenella scatoligenes SK9K4T (94.3 %), Olsenella uli ATCC 49627T (93.5 %), Olsenella umbonata lac31T (93.4 %) and Olsenella profusa D315A-29T (93.3 %).
31135332	10	40	theme	name	1124:1127	arg1	sp					1147:1148	the name Olsenellafaecalis sp	1120:1148	the name Olsenellafaecalis sp	1120:1148	Based on phylogenetic, physiological and chemotaxonomic characteristics, strain KGMB04489T is considered to represent a novel species within the genus Olsenella, for which the name Olsenellafaecalis sp.
31135332	4	41	theme	16S	294:296	arg1	similarity					317:326	16S rRNA gene sequence similarity	294:326	16S rRNA gene sequence similarity	294:326	On the basis of 16S rRNA gene sequence similarity, strain KGMB04489T belonged to the genus Olsenella and was most closely related to Olsenella scatoligenes SK9K4T (94.3 %), Olsenella uli ATCC 49627T (93.5 %), Olsenella umbonata lac31T (93.4 %) and Olsenella profusa D315A-29T (93.3 %).
31135332	4	42	dep	related	400:406	arg1	%					560:560	93.3 %	555:560	93.3 %	555:560	On the basis of 16S rRNA gene sequence similarity, strain KGMB04489T belonged to the genus Olsenella and was most closely related to Olsenella scatoligenes SK9K4T (94.3 %), Olsenella uli ATCC 49627T (93.5 %), Olsenella umbonata lac31T (93.4 %) and Olsenella profusa D315A-29T (93.3 %).
31135332	10	43	theme	physiological	971:983	arg1	characteristics					1004:1018	phylogenetic, physiological and chemotaxonomic characteristics	957:1018	phylogenetic, physiological and chemotaxonomic characteristics	957:1018	Based on phylogenetic, physiological and chemotaxonomic characteristics, strain KGMB04489T is considered to represent a novel species within the genus Olsenella, for which the name Olsenellafaecalis sp.
31135332	1	44	theme	anaerobic	32:40	arg1	actinobacterium					42:56	an anaerobic actinobacterium	29:56	an anaerobic actinobacterium	29:56	nov., an anaerobic actinobacterium isolated from human faeces.
31135332	1	44	theme	anaerobic	32:40	arg1	nov.					23:26	nov.	23:26	nov.	23:26	nov., an anaerobic actinobacterium isolated from human faeces.
31135332	4	45	theme	sequence	308:315	arg1	similarity					317:326	16S rRNA gene sequence similarity	294:326	16S rRNA gene sequence similarity	294:326	On the basis of 16S rRNA gene sequence similarity, strain KGMB04489T belonged to the genus Olsenella and was most closely related to Olsenella scatoligenes SK9K4T (94.3 %), Olsenella uli ATCC 49627T (93.5 %), Olsenella umbonata lac31T (93.4 %) and Olsenella profusa D315A-29T (93.3 %).
31135332	10	46	theme	genus	1093:1097	arg1	Olsenella					1099:1107	the genus Olsenella	1089:1107	the genus Olsenella	1089:1107	Based on phylogenetic, physiological and chemotaxonomic characteristics, strain KGMB04489T is considered to represent a novel species within the genus Olsenella, for which the name Olsenellafaecalis sp.
31135332	9	47	theme	unidentified	928:939	arg1	lipid					941:945	an unidentified lipid	925:945	an unidentified lipid	925:945	The polar lipids consisted of an unidentified phospholipid, six unidentified glycolipids and an unidentified lipid.
31135332	7	48	theme	cellular	648:655	arg1	C18 					695:698	C18 	695:698	C18 	695:698	The major cellular fatty acids of strain KGMB04489T were C18 : 1cis9, C18 : 1cis9 DMA and C16 : 0.
31135332	7	48	theme	cellular	648:655	arg1	acids					663:667	The major cellular fatty acids	638:667	The major cellular fatty acids of strain KGMB04489T	638:688	The major cellular fatty acids of strain KGMB04489T were C18 : 1cis9, C18 : 1cis9 DMA and C16 : 0.
30882297	12	0	theme	gen.	1885:1888	arg1	sp					1896:1897	sp	1896:1897	sp	1896:1897	Based on the phylogenetic and phenotypic evidence, we propose that the unknown bacterium be classified as Suicoccus acidiformans gen. nov., sp.
30882297	12	0	theme	gen.	1885:1888	arg1	nov.					1890:1893	Suicoccus acidiformans gen. nov.	1862:1893	Suicoccus acidiformans gen. nov.	1862:1893	Based on the phylogenetic and phenotypic evidence, we propose that the unknown bacterium be classified as Suicoccus acidiformans gen. nov., sp.
30882297	12	0	theme	gen.	1885:1888	arg1	bacterium					1835:1843	the unknown bacterium	1823:1843	the unknown bacterium	1823:1843	Based on the phylogenetic and phenotypic evidence, we propose that the unknown bacterium be classified as Suicoccus acidiformans gen. nov., sp.
30882297	7	1	from	species	1247:1253	arg1	Aerococcaceae					1229:1241	the family Aerococcaceae	1218:1241	the family Aerococcaceae	1218:1241	The genomic OrthoANI values between strain ZY16052T and type species of related genera in the family Aerococcaceae and species in the genus Facklamia were ≤67.77 and ≤68.11 %, respectively.
30882297	4	2	theme	strain	507:512	arg1	ZY16052T					514:521	strain ZY16052T	507:521	strain ZY16052T	507:521	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain ZY16052T formed a separated evolutionary lineage from recognized genera of the family Aerococcaceae and shared low similarity to its closest related species Facklamiasourekii (93.8 %) and Ignavigranum ruoffiae (93.4 %).
30882297	9	3	theme	ante	1476:1479	arg1	 0					1473:1474	 0 ante	1473:1479	 0 ante	1473:1479	The predominant fatty acids (>5 %) were C16 : 0, C18 : 1ω9c, C14 : 0 and summed feature 5 (C18 : 2ω6,9c and/or C18 : 0 ante).
30882297	1	4	theme	sick	60:63	arg1	pig					65:67	a sick pig	58:67	a sick pig	58:67	nov., isolated from a sick pig.
30882297	2	5	theme	anaerobic	148:156	arg1	coccus					158:163	A Gram-stain-positive, non-spore-forming, catalase-positive and facultatively anaerobic coccus	70:163	A Gram-stain-positive, non-spore-forming, catalase-positive and facultatively anaerobic coccus	70:163	A Gram-stain-positive, non-spore-forming, catalase-positive and facultatively anaerobic coccus, designated ZY16052T, was isolated from mesenteric lymph nodes of a sick piglet in Kunming, Yunnan Province, PR China and its taxonomic position was studied by following a polyphasic approach.
30882297	10	6	theme	phosphatidic	1598:1609	arg1	acid					1611:1614	phosphatidic acid	1598:1614	phosphatidic acid	1598:1614	The major polar lipids were digalactosyldiacylglycerol, phosphatidylglycerol, diacylglycerols, triacylglycerol and phosphatidic acid.
30882297	7	7	from	ZY16052T	1171:1178	arg1	Aerococcaceae					1229:1241	the family Aerococcaceae	1218:1241	the family Aerococcaceae	1218:1241	The genomic OrthoANI values between strain ZY16052T and type species of related genera in the family Aerococcaceae and species in the genus Facklamia were ≤67.77 and ≤68.11 %, respectively.
30882297	6	8	theme	low	1086:1088	arg1	similarity					1090:1099	very low similarity	1081:1099	very low similarity ≤77.0 % to related genera	1081:1125	Sequence analysis based on the housekeeping gene rpoA indicated that strain ZY16052T shared very low similarity ≤77.0 % to related genera.
30882297	4	9	theme	family	593:598	arg1	Aerococcaceae					600:612	the family Aerococcaceae	589:612	the family Aerococcaceae	589:612	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain ZY16052T formed a separated evolutionary lineage from recognized genera of the family Aerococcaceae and shared low similarity to its closest related species Facklamiasourekii (93.8 %) and Ignavigranum ruoffiae (93.4 %).
30882297	9	10	theme	predominant	1361:1371	arg1	acids					1379:1383	The predominant fatty acids	1357:1383	The predominant fatty acids (>5 %)	1357:1390	The predominant fatty acids (>5 %) were C16 : 0, C18 : 1ω9c, C14 : 0 and summed feature 5 (C18 : 2ω6,9c and/or C18 : 0 ante).
30882297	9	10	theme	predominant	1361:1371	arg1	%					1389:1389	>5 %	1386:1389	>5 %	1386:1389	The predominant fatty acids (>5 %) were C16 : 0, C18 : 1ω9c, C14 : 0 and summed feature 5 (C18 : 2ω6,9c and/or C18 : 0 ante).
30882297	9	10	theme	predominant	1361:1371	arg1	C16 					1397:1400	C16 	1397:1400	C16 	1397:1400	The predominant fatty acids (>5 %) were C16 : 0, C18 : 1ω9c, C14 : 0 and summed feature 5 (C18 : 2ω6,9c and/or C18 : 0 ante).
30882297	6	11	theme	strain	1058:1063	arg1	ZY16052T					1065:1072	strain ZY16052T	1058:1072	strain ZY16052T	1058:1072	Sequence analysis based on the housekeeping gene rpoA indicated that strain ZY16052T shared very low similarity ≤77.0 % to related genera.
30882297	4	12	theme	recognized	568:577	arg1	genera					579:584	recognized genera	568:584	recognized genera of the family Aerococcaceae	568:612	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain ZY16052T formed a separated evolutionary lineage from recognized genera of the family Aerococcaceae and shared low similarity to its closest related species Facklamiasourekii (93.8 %) and Ignavigranum ruoffiae (93.4 %).
30882297	12	13	theme	phenotypic	1786:1795	arg1	evidence					1797:1804	the phylogenetic and phenotypic evidence	1765:1804	the phylogenetic and phenotypic evidence	1765:1804	Based on the phylogenetic and phenotypic evidence, we propose that the unknown bacterium be classified as Suicoccus acidiformans gen. nov., sp.
30882297	11	14	theme	type	1746:1749	arg1	A1a					1751:1753	peptidoglycan type A1a	1732:1753	peptidoglycan type A1a	1732:1753	The peptidoglycan contained the amino acids lysine, glycine, alanine and glutamic acid, which is characteristic of peptidoglycan type A1a.
30882297	5	15	theme	gene	782:785	arg1	recA					787:790	the housekeeping gene recA	765:790	the housekeeping gene recA	765:790	Phylogenetic analysis based on the housekeeping gene recA indicated that strain ZY16052T represented a deep and distinct evolutionary lineage, and was well separated from all genera in the family Aerococcaceae, with very low sequence similarity(≤73.2 %).
30882297	4	16	theme	closest	647:653	arg1	Facklamiasourekii					671:687	Facklamiasourekii	671:687	Facklamiasourekii (93.8 %)	671:696	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain ZY16052T formed a separated evolutionary lineage from recognized genera of the family Aerococcaceae and shared low similarity to its closest related species Facklamiasourekii (93.8 %) and Ignavigranum ruoffiae (93.4 %).
30882297	4	16	theme	closest	647:653	arg1	ruoffiae					715:722	Ignavigranum ruoffiae	702:722	Ignavigranum ruoffiae (93.4 %)	702:731	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain ZY16052T formed a separated evolutionary lineage from recognized genera of the family Aerococcaceae and shared low similarity to its closest related species Facklamiasourekii (93.8 %) and Ignavigranum ruoffiae (93.4 %).
30882297	4	16	theme	closest	647:653	arg1	species					663:669	its closest related species	643:669	its closest related species Facklamiasourekii (93.8 %) and Ignavigranum ruoffiae (93.4 %)	643:731	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain ZY16052T formed a separated evolutionary lineage from recognized genera of the family Aerococcaceae and shared low similarity to its closest related species Facklamiasourekii (93.8 %) and Ignavigranum ruoffiae (93.4 %).
30882297	14	17	theme	2017017T=DSM	1970:1981	arg1	ZY16052T					1950:1957	ZY16052T	1950:1957	ZY16052T (=CCTCC AB 2017017T=DSM 105755T)	1950:1990	The type strain of Suicoccus acidiformans is ZY16052T (=CCTCC AB 2017017T=DSM 105755T).
30882297	14	17	theme	2017017T=DSM	1970:1981	arg1	105755T					1983:1989	=CCTCC AB 2017017T=DSM 105755T	1960:1989	=CCTCC AB 2017017T=DSM 105755T	1960:1989	The type strain of Suicoccus acidiformans is ZY16052T (=CCTCC AB 2017017T=DSM 105755T).
30882297	5	18	theme	sequence	959:966	arg1	similarity					968:977	very low sequence similarity	950:977	very low sequence similarity(≤73.2 %)	950:986	Phylogenetic analysis based on the housekeeping gene recA indicated that strain ZY16052T represented a deep and distinct evolutionary lineage, and was well separated from all genera in the family Aerococcaceae, with very low sequence similarity(≤73.2 %).
30882297	5	18	theme	sequence	959:966	arg1	%					985:985	≤73.2 %	979:985	≤73.2 %	979:985	Phylogenetic analysis based on the housekeeping gene recA indicated that strain ZY16052T represented a deep and distinct evolutionary lineage, and was well separated from all genera in the family Aerococcaceae, with very low sequence similarity(≤73.2 %).
30882297	2	19	theme	mesenteric	205:214	arg1	nodes					222:226	mesenteric lymph nodes	205:226	mesenteric lymph nodes of a sick piglet in Kunming, Yunnan Province, PR China	205:281	A Gram-stain-positive, non-spore-forming, catalase-positive and facultatively anaerobic coccus, designated ZY16052T, was isolated from mesenteric lymph nodes of a sick piglet in Kunming, Yunnan Province, PR China and its taxonomic position was studied by following a polyphasic approach.
30882297	5	20	theme	distinct	846:853	arg1	lineage					868:874	a deep and distinct evolutionary lineage	835:874	a deep and distinct evolutionary lineage	835:874	Phylogenetic analysis based on the housekeeping gene recA indicated that strain ZY16052T represented a deep and distinct evolutionary lineage, and was well separated from all genera in the family Aerococcaceae, with very low sequence similarity(≤73.2 %).
30882297	7	21	theme	genus	1262:1266	arg1	Facklamia					1268:1276	the genus Facklamia	1258:1276	the genus Facklamia	1258:1276	The genomic OrthoANI values between strain ZY16052T and type species of related genera in the family Aerococcaceae and species in the genus Facklamia were ≤67.77 and ≤68.11 %, respectively.
30882297	2	22	theme	piglet	238:243	arg1	nodes					222:226	mesenteric lymph nodes	205:226	mesenteric lymph nodes of a sick piglet in Kunming, Yunnan Province, PR China	205:281	A Gram-stain-positive, non-spore-forming, catalase-positive and facultatively anaerobic coccus, designated ZY16052T, was isolated from mesenteric lymph nodes of a sick piglet in Kunming, Yunnan Province, PR China and its taxonomic position was studied by following a polyphasic approach.
30882297	10	23	theme	polar	1493:1497	arg1	digalactosyldiacylglycerol					1511:1536	digalactosyldiacylglycerol	1511:1536	digalactosyldiacylglycerol	1511:1536	The major polar lipids were digalactosyldiacylglycerol, phosphatidylglycerol, diacylglycerols, triacylglycerol and phosphatidic acid.
30882297	10	23	theme	polar	1493:1497	arg1	lipids					1499:1504	The major polar lipids	1483:1504	The major polar lipids	1483:1504	The major polar lipids were digalactosyldiacylglycerol, phosphatidylglycerol, diacylglycerols, triacylglycerol and phosphatidic acid.
30882297	4	24	theme	low	625:627	arg1	similarity					629:638	low similarity	625:638	low similarity to its closest related species Facklamiasourekii (93.8 %) and Ignavigranum ruoffiae (93.4 %)	625:731	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain ZY16052T formed a separated evolutionary lineage from recognized genera of the family Aerococcaceae and shared low similarity to its closest related species Facklamiasourekii (93.8 %) and Ignavigranum ruoffiae (93.4 %).
30882297	7	25	theme	OrthoANI	1140:1147	arg1	values					1149:1154	The genomic OrthoANI values	1128:1154	The genomic OrthoANI values between strain ZY16052T and type species of related genera in the family Aerococcaceae and species in the genus Facklamia	1128:1276	The genomic OrthoANI values between strain ZY16052T and type species of related genera in the family Aerococcaceae and species in the genus Facklamia were ≤67.77 and ≤68.11 %, respectively.
30882297	7	25	theme	OrthoANI	1140:1147	arg1	%					1301:1301	≤67.77 and ≤68.11 %	1283:1301	≤67.77 and ≤68.11 %	1283:1301	The genomic OrthoANI values between strain ZY16052T and type species of related genera in the family Aerococcaceae and species in the genus Facklamia were ≤67.77 and ≤68.11 %, respectively.
30882297	14	26	theme	=CCTCC AB	1960:1968	arg1	ZY16052T					1950:1957	ZY16052T	1950:1957	ZY16052T (=CCTCC AB 2017017T=DSM 105755T)	1950:1990	The type strain of Suicoccus acidiformans is ZY16052T (=CCTCC AB 2017017T=DSM 105755T).
30882297	14	26	theme	=CCTCC AB	1960:1968	arg1	105755T					1983:1989	=CCTCC AB 2017017T=DSM 105755T	1960:1989	=CCTCC AB 2017017T=DSM 105755T	1960:1989	The type strain of Suicoccus acidiformans is ZY16052T (=CCTCC AB 2017017T=DSM 105755T).
30882297	2	27	dep	Province	264:271	arg1	PR					274:275	PR	274:275	PR	274:275	A Gram-stain-positive, non-spore-forming, catalase-positive and facultatively anaerobic coccus, designated ZY16052T, was isolated from mesenteric lymph nodes of a sick piglet in Kunming, Yunnan Province, PR China and its taxonomic position was studied by following a polyphasic approach.
30882297	11	28	dep	acids	1655:1659	arg1	acid					1699:1702	glutamic acid	1690:1702	glutamic acid	1690:1702	The peptidoglycan contained the amino acids lysine, glycine, alanine and glutamic acid, which is characteristic of peptidoglycan type A1a.
30882297	11	28	dep	acids	1655:1659	arg1	glycine					1669:1675	glycine	1669:1675	glycine	1669:1675	The peptidoglycan contained the amino acids lysine, glycine, alanine and glutamic acid, which is characteristic of peptidoglycan type A1a.
30882297	11	28	dep	acids	1655:1659	arg1	acids					1655:1659	the amino acids lysine, glycine, alanine and glutamic acid	1645:1702	the amino acids lysine, glycine, alanine and glutamic acid	1645:1702	The peptidoglycan contained the amino acids lysine, glycine, alanine and glutamic acid, which is characteristic of peptidoglycan type A1a.
30882297	11	28	dep	acids	1655:1659	arg1	lysine					1661:1666	lysine	1661:1666	lysine	1661:1666	The peptidoglycan contained the amino acids lysine, glycine, alanine and glutamic acid, which is characteristic of peptidoglycan type A1a.
30882297	11	28	dep	acids	1655:1659	arg1	alanine					1678:1684	alanine	1678:1684	alanine	1678:1684	The peptidoglycan contained the amino acids lysine, glycine, alanine and glutamic acid, which is characteristic of peptidoglycan type A1a.
30882297	5	29	theme	deep	837:840	arg1	lineage					868:874	a deep and distinct evolutionary lineage	835:874	a deep and distinct evolutionary lineage	835:874	Phylogenetic analysis based on the housekeeping gene recA indicated that strain ZY16052T represented a deep and distinct evolutionary lineage, and was well separated from all genera in the family Aerococcaceae, with very low sequence similarity(≤73.2 %).
30882297	14	30	theme	acidiformans	1934:1945	arg1	ZY16052T					1950:1957	ZY16052T	1950:1957	ZY16052T (=CCTCC AB 2017017T=DSM 105755T)	1950:1990	The type strain of Suicoccus acidiformans is ZY16052T (=CCTCC AB 2017017T=DSM 105755T).
30882297	14	30	theme	acidiformans	1934:1945	arg1	strain					1914:1919	The type strain	1905:1919	The type strain of Suicoccus acidiformans	1905:1945	The type strain of Suicoccus acidiformans is ZY16052T (=CCTCC AB 2017017T=DSM 105755T).
30882297	11	31	theme	A1a	1751:1753	arg1	acids					1655:1659	the amino acids lysine, glycine, alanine and glutamic acid	1645:1702	the amino acids lysine, glycine, alanine and glutamic acid	1645:1702	The peptidoglycan contained the amino acids lysine, glycine, alanine and glutamic acid, which is characteristic of peptidoglycan type A1a.
30882297	11	31	theme	A1a	1751:1753	arg1	characteristic					1714:1727	characteristic	1714:1727	characteristic of peptidoglycan type A1a	1714:1753	The peptidoglycan contained the amino acids lysine, glycine, alanine and glutamic acid, which is characteristic of peptidoglycan type A1a.
30882297	11	32	theme	peptidoglycan	1732:1744	arg1	A1a					1751:1753	peptidoglycan type A1a	1732:1753	peptidoglycan type A1a	1732:1753	The peptidoglycan contained the amino acids lysine, glycine, alanine and glutamic acid, which is characteristic of peptidoglycan type A1a.
30882297	5	33	theme	Phylogenetic	734:745	arg1	analysis					747:754	Phylogenetic analysis	734:754	Phylogenetic analysis based on the housekeeping gene recA	734:790	Phylogenetic analysis based on the housekeeping gene recA indicated that strain ZY16052T represented a deep and distinct evolutionary lineage, and was well separated from all genera in the family Aerococcaceae, with very low sequence similarity(≤73.2 %).
30882297	4	34	theme	rRNA	475:478	arg1	sequences					485:493	16S rRNA gene sequences	471:493	16S rRNA gene sequences	471:493	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain ZY16052T formed a separated evolutionary lineage from recognized genera of the family Aerococcaceae and shared low similarity to its closest related species Facklamiasourekii (93.8 %) and Ignavigranum ruoffiae (93.4 %).
30882297	14	35	theme	type	1909:1912	arg1	ZY16052T					1950:1957	ZY16052T	1950:1957	ZY16052T (=CCTCC AB 2017017T=DSM 105755T)	1950:1990	The type strain of Suicoccus acidiformans is ZY16052T (=CCTCC AB 2017017T=DSM 105755T).
30882297	14	35	theme	type	1909:1912	arg1	strain					1914:1919	The type strain	1905:1919	The type strain of Suicoccus acidiformans	1905:1945	The type strain of Suicoccus acidiformans is ZY16052T (=CCTCC AB 2017017T=DSM 105755T).
30882297	3	36	theme	%	409:409	arg1	w/v					417:419	w/v	417:419	w/v	417:419	Optimal growth was observed at 37 °C, pH 8.0 and 2 % NaCl (w/v) on Columbia agar.
30882297	3	36	theme	%	409:409	arg1	NaCl					411:414	2 % NaCl	407:414	2 % NaCl (w/v)	407:420	Optimal growth was observed at 37 °C, pH 8.0 and 2 % NaCl (w/v) on Columbia agar.
30882297	0	37	theme	gen.	23:26	arg1	sp					34:35	sp	34:35	sp	34:35	Suicoccus acidiformans gen. nov., sp.
30882297	0	37	theme	gen.	23:26	arg1	nov.					28:31	gen. nov.	23:31	gen. nov.	23:31	Suicoccus acidiformans gen. nov., sp.
30882297	9	38	theme	summed	1430:1435	arg1	feature					1437:1443	summed feature	1430:1443	summed feature	1430:1443	The predominant fatty acids (>5 %) were C16 : 0, C18 : 1ω9c, C14 : 0 and summed feature 5 (C18 : 2ω6,9c and/or C18 : 0 ante).
30882297	7	39	theme	genera	1208:1213	arg1	ZY16052T					1171:1178	strain ZY16052T and type species	1164:1195	ZY16052T	1171:1178	The genomic OrthoANI values between strain ZY16052T and type species of related genera in the family Aerococcaceae and species in the genus Facklamia were ≤67.77 and ≤68.11 %, respectively.
30882297	7	39	theme	genera	1208:1213	arg1	species					1189:1195	strain ZY16052T and type species	1164:1195	species	1189:1195	The genomic OrthoANI values between strain ZY16052T and type species of related genera in the family Aerococcaceae and species in the genus Facklamia were ≤67.77 and ≤68.11 %, respectively.
30882297	7	39	theme	genera	1208:1213	arg1	species					1247:1253	species	1247:1253	species	1247:1253	The genomic OrthoANI values between strain ZY16052T and type species of related genera in the family Aerococcaceae and species in the genus Facklamia were ≤67.77 and ≤68.11 %, respectively.
30882297	5	40	theme	low	955:957	arg1	similarity					968:977	very low sequence similarity	950:977	very low sequence similarity(≤73.2 %)	950:986	Phylogenetic analysis based on the housekeeping gene recA indicated that strain ZY16052T represented a deep and distinct evolutionary lineage, and was well separated from all genera in the family Aerococcaceae, with very low sequence similarity(≤73.2 %).
30882297	5	40	theme	low	955:957	arg1	%					985:985	≤73.2 %	979:985	≤73.2 %	979:985	Phylogenetic analysis based on the housekeeping gene recA indicated that strain ZY16052T represented a deep and distinct evolutionary lineage, and was well separated from all genera in the family Aerococcaceae, with very low sequence similarity(≤73.2 %).
30882297	2	41	theme	taxonomic	291:299	arg1	position					301:308	its taxonomic position	287:308	its taxonomic position	287:308	A Gram-stain-positive, non-spore-forming, catalase-positive and facultatively anaerobic coccus, designated ZY16052T, was isolated from mesenteric lymph nodes of a sick piglet in Kunming, Yunnan Province, PR China and its taxonomic position was studied by following a polyphasic approach.
30882297	7	42	theme	type	1184:1187	arg1	species					1189:1195	strain ZY16052T and type species	1164:1195	species	1189:1195	The genomic OrthoANI values between strain ZY16052T and type species of related genera in the family Aerococcaceae and species in the genus Facklamia were ≤67.77 and ≤68.11 %, respectively.
30882297	8	43	theme	42.3 mol	1346:1353	arg1	G+C content					1330:1340	The genomic G+C content	1318:1340	The genomic G+C content	1318:1340	The genomic G+C content was 42.3 mol%.
30882297	8	43	theme	42.3 mol	1346:1353	arg1	%					1354:1354	42.3 mol%	1346:1354	42.3 mol%	1346:1354	The genomic G+C content was 42.3 mol%.
30882297	4	44	theme	evolutionary	542:553	arg1	lineage					555:561	a separated evolutionary lineage	530:561	a separated evolutionary lineage from recognized genera of the family Aerococcaceae	530:612	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain ZY16052T formed a separated evolutionary lineage from recognized genera of the family Aerococcaceae and shared low similarity to its closest related species Facklamiasourekii (93.8 %) and Ignavigranum ruoffiae (93.4 %).
30882297	2	45	theme	non-spore-forming	93:109	arg1	coccus					158:163	A Gram-stain-positive, non-spore-forming, catalase-positive and facultatively anaerobic coccus	70:163	A Gram-stain-positive, non-spore-forming, catalase-positive and facultatively anaerobic coccus	70:163	A Gram-stain-positive, non-spore-forming, catalase-positive and facultatively anaerobic coccus, designated ZY16052T, was isolated from mesenteric lymph nodes of a sick piglet in Kunming, Yunnan Province, PR China and its taxonomic position was studied by following a polyphasic approach.
30882297	3	46	theme	Columbia	425:432	arg1	agar					434:437	Columbia agar	425:437	Columbia agar	425:437	Optimal growth was observed at 37 °C, pH 8.0 and 2 % NaCl (w/v) on Columbia agar.
30882297	5	47	from	genera	909:914	arg1	Aerococcaceae					930:942	the family Aerococcaceae	919:942	the family Aerococcaceae	919:942	Phylogenetic analysis based on the housekeeping gene recA indicated that strain ZY16052T represented a deep and distinct evolutionary lineage, and was well separated from all genera in the family Aerococcaceae, with very low sequence similarity(≤73.2 %).
30882297	12	48	theme	acidiformans	1872:1883	arg1	sp					1896:1897	sp	1896:1897	sp	1896:1897	Based on the phylogenetic and phenotypic evidence, we propose that the unknown bacterium be classified as Suicoccus acidiformans gen. nov., sp.
30882297	12	48	theme	acidiformans	1872:1883	arg1	nov.					1890:1893	Suicoccus acidiformans gen. nov.	1862:1893	Suicoccus acidiformans gen. nov.	1862:1893	Based on the phylogenetic and phenotypic evidence, we propose that the unknown bacterium be classified as Suicoccus acidiformans gen. nov., sp.
30882297	12	48	theme	acidiformans	1872:1883	arg1	bacterium					1835:1843	the unknown bacterium	1823:1843	the unknown bacterium	1823:1843	Based on the phylogenetic and phenotypic evidence, we propose that the unknown bacterium be classified as Suicoccus acidiformans gen. nov., sp.
30882297	7	49	from	values	1149:1154	arg1	Facklamia					1268:1276	the genus Facklamia	1258:1276	the genus Facklamia	1258:1276	The genomic OrthoANI values between strain ZY16052T and type species of related genera in the family Aerococcaceae and species in the genus Facklamia were ≤67.77 and ≤68.11 %, respectively.
30882297	2	50	theme	Gram-stain-positive	72:90	arg1	coccus					158:163	A Gram-stain-positive, non-spore-forming, catalase-positive and facultatively anaerobic coccus	70:163	A Gram-stain-positive, non-spore-forming, catalase-positive and facultatively anaerobic coccus	70:163	A Gram-stain-positive, non-spore-forming, catalase-positive and facultatively anaerobic coccus, designated ZY16052T, was isolated from mesenteric lymph nodes of a sick piglet in Kunming, Yunnan Province, PR China and its taxonomic position was studied by following a polyphasic approach.
30882297	12	51	theme	unknown	1827:1833	arg1	nov.					1890:1893	Suicoccus acidiformans gen. nov.	1862:1893	Suicoccus acidiformans gen. nov.	1862:1893	Based on the phylogenetic and phenotypic evidence, we propose that the unknown bacterium be classified as Suicoccus acidiformans gen. nov., sp.
30882297	12	51	theme	unknown	1827:1833	arg1	bacterium					1835:1843	the unknown bacterium	1823:1843	the unknown bacterium	1823:1843	Based on the phylogenetic and phenotypic evidence, we propose that the unknown bacterium be classified as Suicoccus acidiformans gen. nov., sp.
30882297	11	52	contain	contained	1635:1643	arg2	alanine					1678:1684	alanine	1678:1684	alanine	1678:1684	The peptidoglycan contained the amino acids lysine, glycine, alanine and glutamic acid, which is characteristic of peptidoglycan type A1a.
30882297	11	52	contain	contained	1635:1643	arg2	characteristic					1714:1727	characteristic	1714:1727	characteristic of peptidoglycan type A1a	1714:1753	The peptidoglycan contained the amino acids lysine, glycine, alanine and glutamic acid, which is characteristic of peptidoglycan type A1a.
30882297	11	52	contain	contained	1635:1643	arg1	peptidoglycan					1621:1633	The peptidoglycan	1617:1633	The peptidoglycan	1617:1633	The peptidoglycan contained the amino acids lysine, glycine, alanine and glutamic acid, which is characteristic of peptidoglycan type A1a.
30882297	11	52	contain	contained	1635:1643	arg2	lysine					1661:1666	lysine	1661:1666	lysine	1661:1666	The peptidoglycan contained the amino acids lysine, glycine, alanine and glutamic acid, which is characteristic of peptidoglycan type A1a.
30882297	11	52	contain	contained	1635:1643	arg2	acids					1655:1659	the amino acids lysine, glycine, alanine and glutamic acid	1645:1702	the amino acids lysine, glycine, alanine and glutamic acid	1645:1702	The peptidoglycan contained the amino acids lysine, glycine, alanine and glutamic acid, which is characteristic of peptidoglycan type A1a.
30882297	11	52	contain	contained	1635:1643	arg2	glycine					1669:1675	glycine	1669:1675	glycine	1669:1675	The peptidoglycan contained the amino acids lysine, glycine, alanine and glutamic acid, which is characteristic of peptidoglycan type A1a.
30882297	11	52	contain	contained	1635:1643	arg2	acid					1699:1702	glutamic acid	1690:1702	glutamic acid	1690:1702	The peptidoglycan contained the amino acids lysine, glycine, alanine and glutamic acid, which is characteristic of peptidoglycan type A1a.
30882297	9	53	dep	C18 	1448:1451	arg1	 2ω6,9c					1453:1459	 2ω6,9c	1453:1459	 2ω6,9c	1453:1459	The predominant fatty acids (>5 %) were C16 : 0, C18 : 1ω9c, C14 : 0 and summed feature 5 (C18 : 2ω6,9c and/or C18 : 0 ante).
30882297	9	53	dep	C18 	1448:1451	arg1	 0					1473:1474	 0 ante	1473:1479	 0 ante	1473:1479	The predominant fatty acids (>5 %) were C16 : 0, C18 : 1ω9c, C14 : 0 and summed feature 5 (C18 : 2ω6,9c and/or C18 : 0 ante).
30882297	9	53	dep	C18 	1448:1451	arg1	C18 					1468:1471	C18 	1468:1471	C18 	1468:1471	The predominant fatty acids (>5 %) were C16 : 0, C18 : 1ω9c, C14 : 0 and summed feature 5 (C18 : 2ω6,9c and/or C18 : 0 ante).
30882297	2	54	theme	catalase-positive	112:128	arg1	coccus					158:163	A Gram-stain-positive, non-spore-forming, catalase-positive and facultatively anaerobic coccus	70:163	A Gram-stain-positive, non-spore-forming, catalase-positive and facultatively anaerobic coccus	70:163	A Gram-stain-positive, non-spore-forming, catalase-positive and facultatively anaerobic coccus, designated ZY16052T, was isolated from mesenteric lymph nodes of a sick piglet in Kunming, Yunnan Province, PR China and its taxonomic position was studied by following a polyphasic approach.
30882297	7	55	from	species	1189:1195	arg1	Aerococcaceae					1229:1241	the family Aerococcaceae	1218:1241	the family Aerococcaceae	1218:1241	The genomic OrthoANI values between strain ZY16052T and type species of related genera in the family Aerococcaceae and species in the genus Facklamia were ≤67.77 and ≤68.11 %, respectively.
30882297	4	56	theme	Aerococcaceae	600:612	arg1	genera					579:584	recognized genera	568:584	recognized genera of the family Aerococcaceae	568:612	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain ZY16052T formed a separated evolutionary lineage from recognized genera of the family Aerococcaceae and shared low similarity to its closest related species Facklamiasourekii (93.8 %) and Ignavigranum ruoffiae (93.4 %).
30882297	4	57	from	genera	579:584	arg1	lineage					555:561	a separated evolutionary lineage	530:561	a separated evolutionary lineage from recognized genera of the family Aerococcaceae	530:612	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain ZY16052T formed a separated evolutionary lineage from recognized genera of the family Aerococcaceae and shared low similarity to its closest related species Facklamiasourekii (93.8 %) and Ignavigranum ruoffiae (93.4 %).
30882297	9	58	theme	fatty	1373:1377	arg1	acids					1379:1383	The predominant fatty acids	1357:1383	The predominant fatty acids (>5 %)	1357:1390	The predominant fatty acids (>5 %) were C16 : 0, C18 : 1ω9c, C14 : 0 and summed feature 5 (C18 : 2ω6,9c and/or C18 : 0 ante).
30882297	9	58	theme	fatty	1373:1377	arg1	%					1389:1389	>5 %	1386:1389	>5 %	1386:1389	The predominant fatty acids (>5 %) were C16 : 0, C18 : 1ω9c, C14 : 0 and summed feature 5 (C18 : 2ω6,9c and/or C18 : 0 ante).
30882297	9	58	theme	fatty	1373:1377	arg1	C16 					1397:1400	C16 	1397:1400	C16 	1397:1400	The predominant fatty acids (>5 %) were C16 : 0, C18 : 1ω9c, C14 : 0 and summed feature 5 (C18 : 2ω6,9c and/or C18 : 0 ante).
30882297	11	59	theme	amino	1649:1653	arg1	acids					1655:1659	the amino acids lysine, glycine, alanine and glutamic acid	1645:1702	the amino acids lysine, glycine, alanine and glutamic acid	1645:1702	The peptidoglycan contained the amino acids lysine, glycine, alanine and glutamic acid, which is characteristic of peptidoglycan type A1a.
30882297	11	59	theme	amino	1649:1653	arg1	lysine					1661:1666	lysine	1661:1666	lysine	1661:1666	The peptidoglycan contained the amino acids lysine, glycine, alanine and glutamic acid, which is characteristic of peptidoglycan type A1a.
30882297	11	59	theme	amino	1649:1653	arg1	acid					1699:1702	glutamic acid	1690:1702	glutamic acid	1690:1702	The peptidoglycan contained the amino acids lysine, glycine, alanine and glutamic acid, which is characteristic of peptidoglycan type A1a.
30882297	11	59	theme	amino	1649:1653	arg1	glycine					1669:1675	glycine	1669:1675	glycine	1669:1675	The peptidoglycan contained the amino acids lysine, glycine, alanine and glutamic acid, which is characteristic of peptidoglycan type A1a.
30882297	11	59	theme	amino	1649:1653	arg1	alanine					1678:1684	alanine	1678:1684	alanine	1678:1684	The peptidoglycan contained the amino acids lysine, glycine, alanine and glutamic acid, which is characteristic of peptidoglycan type A1a.
30882297	11	59	theme	amino	1649:1653	arg1	characteristic					1714:1727	characteristic	1714:1727	characteristic of peptidoglycan type A1a	1714:1753	The peptidoglycan contained the amino acids lysine, glycine, alanine and glutamic acid, which is characteristic of peptidoglycan type A1a.
30882297	5	60	theme	strain	807:812	arg1	ZY16052T					814:821	strain ZY16052T	807:821	strain ZY16052T	807:821	Phylogenetic analysis based on the housekeeping gene recA indicated that strain ZY16052T represented a deep and distinct evolutionary lineage, and was well separated from all genera in the family Aerococcaceae, with very low sequence similarity(≤73.2 %).
30882297	5	61	theme	housekeeping	769:780	arg1	recA					787:790	the housekeeping gene recA	765:790	the housekeeping gene recA	765:790	Phylogenetic analysis based on the housekeeping gene recA indicated that strain ZY16052T represented a deep and distinct evolutionary lineage, and was well separated from all genera in the family Aerococcaceae, with very low sequence similarity(≤73.2 %).
30882297	2	62	theme	lymph	216:220	arg1	nodes					222:226	mesenteric lymph nodes	205:226	mesenteric lymph nodes of a sick piglet in Kunming, Yunnan Province, PR China	205:281	A Gram-stain-positive, non-spore-forming, catalase-positive and facultatively anaerobic coccus, designated ZY16052T, was isolated from mesenteric lymph nodes of a sick piglet in Kunming, Yunnan Province, PR China and its taxonomic position was studied by following a polyphasic approach.
30882297	7	63	theme	≤67.77 and	1283:1292	arg1	values					1149:1154	The genomic OrthoANI values	1128:1154	The genomic OrthoANI values between strain ZY16052T and type species of related genera in the family Aerococcaceae and species in the genus Facklamia	1128:1276	The genomic OrthoANI values between strain ZY16052T and type species of related genera in the family Aerococcaceae and species in the genus Facklamia were ≤67.77 and ≤68.11 %, respectively.
30882297	7	63	theme	≤67.77 and	1283:1292	arg1	%					1301:1301	≤67.77 and ≤68.11 %	1283:1301	≤67.77 and ≤68.11 %	1283:1301	The genomic OrthoANI values between strain ZY16052T and type species of related genera in the family Aerococcaceae and species in the genus Facklamia were ≤67.77 and ≤68.11 %, respectively.
30882297	5	64	theme	evolutionary	855:866	arg1	lineage					868:874	a deep and distinct evolutionary lineage	835:874	a deep and distinct evolutionary lineage	835:874	Phylogenetic analysis based on the housekeeping gene recA indicated that strain ZY16052T represented a deep and distinct evolutionary lineage, and was well separated from all genera in the family Aerococcaceae, with very low sequence similarity(≤73.2 %).
30882297	6	65	theme	related	1112:1118	arg1	genera					1120:1125	related genera	1112:1125	related genera	1112:1125	Sequence analysis based on the housekeeping gene rpoA indicated that strain ZY16052T shared very low similarity ≤77.0 % to related genera.
30882297	4	66	theme	related	655:661	arg1	Facklamiasourekii					671:687	Facklamiasourekii	671:687	Facklamiasourekii (93.8 %)	671:696	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain ZY16052T formed a separated evolutionary lineage from recognized genera of the family Aerococcaceae and shared low similarity to its closest related species Facklamiasourekii (93.8 %) and Ignavigranum ruoffiae (93.4 %).
30882297	4	66	theme	related	655:661	arg1	ruoffiae					715:722	Ignavigranum ruoffiae	702:722	Ignavigranum ruoffiae (93.4 %)	702:731	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain ZY16052T formed a separated evolutionary lineage from recognized genera of the family Aerococcaceae and shared low similarity to its closest related species Facklamiasourekii (93.8 %) and Ignavigranum ruoffiae (93.4 %).
30882297	4	66	theme	related	655:661	arg1	species					663:669	its closest related species	643:669	its closest related species Facklamiasourekii (93.8 %) and Ignavigranum ruoffiae (93.4 %)	643:731	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain ZY16052T formed a separated evolutionary lineage from recognized genera of the family Aerococcaceae and shared low similarity to its closest related species Facklamiasourekii (93.8 %) and Ignavigranum ruoffiae (93.4 %).
30882297	7	67	theme	genomic	1132:1138	arg1	values					1149:1154	The genomic OrthoANI values	1128:1154	The genomic OrthoANI values between strain ZY16052T and type species of related genera in the family Aerococcaceae and species in the genus Facklamia	1128:1276	The genomic OrthoANI values between strain ZY16052T and type species of related genera in the family Aerococcaceae and species in the genus Facklamia were ≤67.77 and ≤68.11 %, respectively.
30882297	7	67	theme	genomic	1132:1138	arg1	%					1301:1301	≤67.77 and ≤68.11 %	1283:1301	≤67.77 and ≤68.11 %	1283:1301	The genomic OrthoANI values between strain ZY16052T and type species of related genera in the family Aerococcaceae and species in the genus Facklamia were ≤67.77 and ≤68.11 %, respectively.
30882297	2	68	theme	sick	233:236	arg1	piglet					238:243	a sick piglet	231:243	a sick piglet in Kunming, Yunnan Province, PR China	231:281	A Gram-stain-positive, non-spore-forming, catalase-positive and facultatively anaerobic coccus, designated ZY16052T, was isolated from mesenteric lymph nodes of a sick piglet in Kunming, Yunnan Province, PR China and its taxonomic position was studied by following a polyphasic approach.
30882297	4	69	theme	Phylogenetic	440:451	arg1	analysis					453:460	Phylogenetic analysis	440:460	Phylogenetic analysis based on 16S rRNA gene sequences	440:493	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain ZY16052T formed a separated evolutionary lineage from recognized genera of the family Aerococcaceae and shared low similarity to its closest related species Facklamiasourekii (93.8 %) and Ignavigranum ruoffiae (93.4 %).
30882297	9	70	dep	 0	1423:1424	arg1	5					1445:1445	5	1445:1445	5	1445:1445	The predominant fatty acids (>5 %) were C16 : 0, C18 : 1ω9c, C14 : 0 and summed feature 5 (C18 : 2ω6,9c and/or C18 : 0 ante).
30882297	12	71	theme	phylogenetic	1769:1780	arg1	evidence					1797:1804	the phylogenetic and phenotypic evidence	1765:1804	the phylogenetic and phenotypic evidence	1765:1804	Based on the phylogenetic and phenotypic evidence, we propose that the unknown bacterium be classified as Suicoccus acidiformans gen. nov., sp.
30882297	2	72	from	China	277:281	arg1	nodes					222:226	mesenteric lymph nodes	205:226	mesenteric lymph nodes of a sick piglet in Kunming, Yunnan Province, PR China	205:281	A Gram-stain-positive, non-spore-forming, catalase-positive and facultatively anaerobic coccus, designated ZY16052T, was isolated from mesenteric lymph nodes of a sick piglet in Kunming, Yunnan Province, PR China and its taxonomic position was studied by following a polyphasic approach.
30882297	9	73	dep	C16 	1397:1400	arg1	 0					1423:1424	 0	1423:1424	 0	1423:1424	The predominant fatty acids (>5 %) were C16 : 0, C18 : 1ω9c, C14 : 0 and summed feature 5 (C18 : 2ω6,9c and/or C18 : 0 ante).
30882297	9	73	dep	C16 	1397:1400	arg1	feature					1437:1443	summed feature	1430:1443	summed feature	1430:1443	The predominant fatty acids (>5 %) were C16 : 0, C18 : 1ω9c, C14 : 0 and summed feature 5 (C18 : 2ω6,9c and/or C18 : 0 ante).
30882297	9	73	dep	C16 	1397:1400	arg1	C14 					1418:1421	C14 	1418:1421	C14 	1418:1421	The predominant fatty acids (>5 %) were C16 : 0, C18 : 1ω9c, C14 : 0 and summed feature 5 (C18 : 2ω6,9c and/or C18 : 0 ante).
30882297	9	73	dep	C16 	1397:1400	arg1	C18 					1448:1451	C18 	1448:1451	C18 	1448:1451	The predominant fatty acids (>5 %) were C16 : 0, C18 : 1ω9c, C14 : 0 and summed feature 5 (C18 : 2ω6,9c and/or C18 : 0 ante).
30882297	9	73	dep	C16 	1397:1400	arg1	C18 					1406:1409	C18 	1406:1409	C18 	1406:1409	The predominant fatty acids (>5 %) were C16 : 0, C18 : 1ω9c, C14 : 0 and summed feature 5 (C18 : 2ω6,9c and/or C18 : 0 ante).
30882297	6	74	theme	housekeeping	1020:1031	arg1	rpoA					1038:1041	the housekeeping gene rpoA	1016:1041	the housekeeping gene rpoA	1016:1041	Sequence analysis based on the housekeeping gene rpoA indicated that strain ZY16052T shared very low similarity ≤77.0 % to related genera.
30882297	6	75	theme	Sequence	989:996	arg1	analysis					998:1005	Sequence analysis	989:1005	Sequence analysis based on the housekeeping gene rpoA	989:1041	Sequence analysis based on the housekeeping gene rpoA indicated that strain ZY16052T shared very low similarity ≤77.0 % to related genera.
30882297	14	76	theme	Suicoccus	1924:1932	arg1	acidiformans					1934:1945	Suicoccus acidiformans	1924:1945	Suicoccus acidiformans	1924:1945	The type strain of Suicoccus acidiformans is ZY16052T (=CCTCC AB 2017017T=DSM 105755T).
30882297	4	77	theme	Ignavigranum	702:713	arg1	%					730:730	93.4 %	725:730	93.4 %	725:730	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain ZY16052T formed a separated evolutionary lineage from recognized genera of the family Aerococcaceae and shared low similarity to its closest related species Facklamiasourekii (93.8 %) and Ignavigranum ruoffiae (93.4 %).
30882297	4	77	theme	Ignavigranum	702:713	arg1	species					663:669	its closest related species	643:669	its closest related species Facklamiasourekii (93.8 %) and Ignavigranum ruoffiae (93.4 %)	643:731	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain ZY16052T formed a separated evolutionary lineage from recognized genera of the family Aerococcaceae and shared low similarity to its closest related species Facklamiasourekii (93.8 %) and Ignavigranum ruoffiae (93.4 %).
30882297	4	77	theme	Ignavigranum	702:713	arg1	ruoffiae					715:722	Ignavigranum ruoffiae	702:722	Ignavigranum ruoffiae (93.4 %)	702:731	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain ZY16052T formed a separated evolutionary lineage from recognized genera of the family Aerococcaceae and shared low similarity to its closest related species Facklamiasourekii (93.8 %) and Ignavigranum ruoffiae (93.4 %).
30882297	4	78	dep	species	663:669	arg1	%					730:730	93.4 %	725:730	93.4 %	725:730	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain ZY16052T formed a separated evolutionary lineage from recognized genera of the family Aerococcaceae and shared low similarity to its closest related species Facklamiasourekii (93.8 %) and Ignavigranum ruoffiae (93.4 %).
30882297	4	78	dep	species	663:669	arg1	Facklamiasourekii					671:687	Facklamiasourekii	671:687	Facklamiasourekii (93.8 %)	671:696	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain ZY16052T formed a separated evolutionary lineage from recognized genera of the family Aerococcaceae and shared low similarity to its closest related species Facklamiasourekii (93.8 %) and Ignavigranum ruoffiae (93.4 %).
30882297	4	78	dep	species	663:669	arg1	%					695:695	93.8 %	690:695	93.8 %	690:695	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain ZY16052T formed a separated evolutionary lineage from recognized genera of the family Aerococcaceae and shared low similarity to its closest related species Facklamiasourekii (93.8 %) and Ignavigranum ruoffiae (93.4 %).
30882297	4	78	dep	species	663:669	arg1	species					663:669	its closest related species	643:669	its closest related species Facklamiasourekii (93.8 %) and Ignavigranum ruoffiae (93.4 %)	643:731	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain ZY16052T formed a separated evolutionary lineage from recognized genera of the family Aerococcaceae and shared low similarity to its closest related species Facklamiasourekii (93.8 %) and Ignavigranum ruoffiae (93.4 %).
30882297	4	78	dep	species	663:669	arg1	ruoffiae					715:722	Ignavigranum ruoffiae	702:722	Ignavigranum ruoffiae (93.4 %)	702:731	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain ZY16052T formed a separated evolutionary lineage from recognized genera of the family Aerococcaceae and shared low similarity to its closest related species Facklamiasourekii (93.8 %) and Ignavigranum ruoffiae (93.4 %).
30882297	2	79	from	nodes	222:226	arg1	China					277:281	China	277:281	China	277:281	A Gram-stain-positive, non-spore-forming, catalase-positive and facultatively anaerobic coccus, designated ZY16052T, was isolated from mesenteric lymph nodes of a sick piglet in Kunming, Yunnan Province, PR China and its taxonomic position was studied by following a polyphasic approach.
30882297	7	80	theme	family	1222:1227	arg1	Aerococcaceae					1229:1241	the family Aerococcaceae	1218:1241	the family Aerococcaceae	1218:1241	The genomic OrthoANI values between strain ZY16052T and type species of related genera in the family Aerococcaceae and species in the genus Facklamia were ≤67.77 and ≤68.11 %, respectively.
30882297	3	81	theme	Optimal	358:364	arg1	growth					366:371	Optimal growth	358:371	Optimal growth	358:371	Optimal growth was observed at 37 °C, pH 8.0 and 2 % NaCl (w/v) on Columbia agar.
30882297	4	82	theme	gene	480:483	arg1	sequences					485:493	16S rRNA gene sequences	471:493	16S rRNA gene sequences	471:493	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain ZY16052T formed a separated evolutionary lineage from recognized genera of the family Aerococcaceae and shared low similarity to its closest related species Facklamiasourekii (93.8 %) and Ignavigranum ruoffiae (93.4 %).
30882297	2	83	theme	polyphasic	337:346	arg1	approach					348:355	a polyphasic approach	335:355	a polyphasic approach	335:355	A Gram-stain-positive, non-spore-forming, catalase-positive and facultatively anaerobic coccus, designated ZY16052T, was isolated from mesenteric lymph nodes of a sick piglet in Kunming, Yunnan Province, PR China and its taxonomic position was studied by following a polyphasic approach.
30882297	10	84	theme	major	1487:1491	arg1	digalactosyldiacylglycerol					1511:1536	digalactosyldiacylglycerol	1511:1536	digalactosyldiacylglycerol	1511:1536	The major polar lipids were digalactosyldiacylglycerol, phosphatidylglycerol, diacylglycerols, triacylglycerol and phosphatidic acid.
30882297	10	84	theme	major	1487:1491	arg1	lipids					1499:1504	The major polar lipids	1483:1504	The major polar lipids	1483:1504	The major polar lipids were digalactosyldiacylglycerol, phosphatidylglycerol, diacylglycerols, triacylglycerol and phosphatidic acid.
30882297	3	85	located	observed	377:384	arg1	agar					434:437	Columbia agar	425:437	Columbia agar	425:437	Optimal growth was observed at 37 °C, pH 8.0 and 2 % NaCl (w/v) on Columbia agar.
30882297	3	85	located	observed	377:384	arg1	37 °C					389:393	37 °C	389:393	37 °C	389:393	Optimal growth was observed at 37 °C, pH 8.0 and 2 % NaCl (w/v) on Columbia agar.
30882297	3	85	located	observed	377:384	arg1	pH					396:397	pH 8.0	396:401	pH 8.0	396:401	Optimal growth was observed at 37 °C, pH 8.0 and 2 % NaCl (w/v) on Columbia agar.
30882297	3	85	located	observed	377:384	arg1	w/v					417:419	w/v	417:419	w/v	417:419	Optimal growth was observed at 37 °C, pH 8.0 and 2 % NaCl (w/v) on Columbia agar.
30882297	3	85	located	observed	377:384	arg2	growth					366:371	Optimal growth	358:371	Optimal growth	358:371	Optimal growth was observed at 37 °C, pH 8.0 and 2 % NaCl (w/v) on Columbia agar.
30882297	3	85	located	observed	377:384	arg1	NaCl					411:414	2 % NaCl	407:414	2 % NaCl (w/v)	407:420	Optimal growth was observed at 37 °C, pH 8.0 and 2 % NaCl (w/v) on Columbia agar.
30882297	2	86	from	piglet	238:243	arg1	China					277:281	China	277:281	China	277:281	A Gram-stain-positive, non-spore-forming, catalase-positive and facultatively anaerobic coccus, designated ZY16052T, was isolated from mesenteric lymph nodes of a sick piglet in Kunming, Yunnan Province, PR China and its taxonomic position was studied by following a polyphasic approach.
30882297	7	87	theme	related	1200:1206	arg1	genera					1208:1213	related genera	1200:1213	related genera in the family Aerococcaceae	1200:1241	The genomic OrthoANI values between strain ZY16052T and type species of related genera in the family Aerococcaceae and species in the genus Facklamia were ≤67.77 and ≤68.11 %, respectively.
30882297	2	88	attach	isolated	191:198	arg1	nodes					222:226	mesenteric lymph nodes	205:226	mesenteric lymph nodes of a sick piglet in Kunming, Yunnan Province, PR China	205:281	A Gram-stain-positive, non-spore-forming, catalase-positive and facultatively anaerobic coccus, designated ZY16052T, was isolated from mesenteric lymph nodes of a sick piglet in Kunming, Yunnan Province, PR China and its taxonomic position was studied by following a polyphasic approach.
30882297	2	88	attach	isolated	191:198	arg2	coccus					158:163	A Gram-stain-positive, non-spore-forming, catalase-positive and facultatively anaerobic coccus	70:163	A Gram-stain-positive, non-spore-forming, catalase-positive and facultatively anaerobic coccus	70:163	A Gram-stain-positive, non-spore-forming, catalase-positive and facultatively anaerobic coccus, designated ZY16052T, was isolated from mesenteric lymph nodes of a sick piglet in Kunming, Yunnan Province, PR China and its taxonomic position was studied by following a polyphasic approach.
30882297	3	89	theme	2 	407:408	arg1	%					409:409	%	409:409	%	409:409	Optimal growth was observed at 37 °C, pH 8.0 and 2 % NaCl (w/v) on Columbia agar.
30882297	7	90	from	genera	1208:1213	arg1	Aerococcaceae					1229:1241	the family Aerococcaceae	1218:1241	the family Aerococcaceae	1218:1241	The genomic OrthoANI values between strain ZY16052T and type species of related genera in the family Aerococcaceae and species in the genus Facklamia were ≤67.77 and ≤68.11 %, respectively.
30882297	4	91	theme	16S	471:473	arg1	sequences					485:493	16S rRNA gene sequences	471:493	16S rRNA gene sequences	471:493	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain ZY16052T formed a separated evolutionary lineage from recognized genera of the family Aerococcaceae and shared low similarity to its closest related species Facklamiasourekii (93.8 %) and Ignavigranum ruoffiae (93.4 %).
30882297	6	92	theme	gene	1033:1036	arg1	rpoA					1038:1041	the housekeeping gene rpoA	1016:1041	the housekeeping gene rpoA	1016:1041	Sequence analysis based on the housekeeping gene rpoA indicated that strain ZY16052T shared very low similarity ≤77.0 % to related genera.
30882297	4	93	theme	separated	532:540	arg1	lineage					555:561	a separated evolutionary lineage	530:561	a separated evolutionary lineage from recognized genera of the family Aerococcaceae	530:612	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain ZY16052T formed a separated evolutionary lineage from recognized genera of the family Aerococcaceae and shared low similarity to its closest related species Facklamiasourekii (93.8 %) and Ignavigranum ruoffiae (93.4 %).
30882297	8	94	theme	genomic	1322:1328	arg1	G+C content					1330:1340	The genomic G+C content	1318:1340	The genomic G+C content	1318:1340	The genomic G+C content was 42.3 mol%.
30882297	8	94	theme	genomic	1322:1328	arg1	%					1354:1354	42.3 mol%	1346:1354	42.3 mol%	1346:1354	The genomic G+C content was 42.3 mol%.
30882297	11	95	theme	glutamic	1690:1697	arg1	acid					1699:1702	glutamic acid	1690:1702	glutamic acid	1690:1702	The peptidoglycan contained the amino acids lysine, glycine, alanine and glutamic acid, which is characteristic of peptidoglycan type A1a.
30882297	11	95	theme	glutamic	1690:1697	arg1	acids					1655:1659	the amino acids lysine, glycine, alanine and glutamic acid	1645:1702	the amino acids lysine, glycine, alanine and glutamic acid	1645:1702	The peptidoglycan contained the amino acids lysine, glycine, alanine and glutamic acid, which is characteristic of peptidoglycan type A1a.
30882297	12	96	theme	Suicoccus	1862:1870	arg1	sp					1896:1897	sp	1896:1897	sp	1896:1897	Based on the phylogenetic and phenotypic evidence, we propose that the unknown bacterium be classified as Suicoccus acidiformans gen. nov., sp.
30882297	12	96	theme	Suicoccus	1862:1870	arg1	nov.					1890:1893	Suicoccus acidiformans gen. nov.	1862:1893	Suicoccus acidiformans gen. nov.	1862:1893	Based on the phylogenetic and phenotypic evidence, we propose that the unknown bacterium be classified as Suicoccus acidiformans gen. nov., sp.
30882297	12	96	theme	Suicoccus	1862:1870	arg1	bacterium					1835:1843	the unknown bacterium	1823:1843	the unknown bacterium	1823:1843	Based on the phylogenetic and phenotypic evidence, we propose that the unknown bacterium be classified as Suicoccus acidiformans gen. nov., sp.
30882297	5	97	theme	family	923:928	arg1	Aerococcaceae					930:942	the family Aerococcaceae	919:942	the family Aerococcaceae	919:942	Phylogenetic analysis based on the housekeeping gene recA indicated that strain ZY16052T represented a deep and distinct evolutionary lineage, and was well separated from all genera in the family Aerococcaceae, with very low sequence similarity(≤73.2 %).
30882297	7	98	from	Aerococcaceae	1229:1241	arg1	ZY16052T					1171:1178	strain ZY16052T and type species	1164:1195	ZY16052T	1171:1178	The genomic OrthoANI values between strain ZY16052T and type species of related genera in the family Aerococcaceae and species in the genus Facklamia were ≤67.77 and ≤68.11 %, respectively.
30882297	7	98	from	Aerococcaceae	1229:1241	arg1	species					1189:1195	strain ZY16052T and type species	1164:1195	species	1189:1195	The genomic OrthoANI values between strain ZY16052T and type species of related genera in the family Aerococcaceae and species in the genus Facklamia were ≤67.77 and ≤68.11 %, respectively.
30882297	7	98	from	Aerococcaceae	1229:1241	arg1	species					1247:1253	species	1247:1253	species	1247:1253	The genomic OrthoANI values between strain ZY16052T and type species of related genera in the family Aerococcaceae and species in the genus Facklamia were ≤67.77 and ≤68.11 %, respectively.
30882297	7	99	theme	strain	1164:1169	arg1	ZY16052T					1171:1178	strain ZY16052T and type species	1164:1195	ZY16052T	1171:1178	The genomic OrthoANI values between strain ZY16052T and type species of related genera in the family Aerococcaceae and species in the genus Facklamia were ≤67.77 and ≤68.11 %, respectively.
31428526	7	0	theme	proangiogenic	1282:1294	arg1	profile					1296:1302	a proangiogenic profile	1280:1302	a proangiogenic profile	1280:1302	Moreover, experiments performed in vitro using semen fucosylated clusterin and monocyte derived macrophages showed that the interaction of semen clusterin with DC-SIGN promoted a proangiogenic profile, characterized by a high production of VEGF, IL-8 and TNF-α.
31428526	7	1	theme	fucosylated	1156:1166	arg1	clusterin					1168:1176	semen fucosylated clusterin	1150:1176	semen fucosylated clusterin	1150:1176	Moreover, experiments performed in vitro using semen fucosylated clusterin and monocyte derived macrophages showed that the interaction of semen clusterin with DC-SIGN promoted a proangiogenic profile, characterized by a high production of VEGF, IL-8 and TNF-α.
31428526	3	2	from	present	528:534	arg1	serum					563:567	serum	563:567	serum	563:567	We have previously described a novel clusterin glycoform present in human semen, but not in serum, highly enriched in terminal fucose motifs.
31428526	3	2	from	present	528:534	arg1	semen					545:549	human semen	539:549	human semen	539:549	We have previously described a novel clusterin glycoform present in human semen, but not in serum, highly enriched in terminal fucose motifs.
31428526	2	3	theme	cancers	370:376	arg1	types					361:365	many types	356:365	many types of cancers	356:376	Clusterin is overexpressed in many types of cancers and a large body of evidence suggests that it promotes carcinogenesis and tumor progression.
31428526	1	4	theme	protein	275:281	arg1	clearance					283:291	unfolded protein clearance	266:291	unfolded protein clearance	266:291	Clusterin is a glycoprotein able to mediate different physiological functions such as control of complement activation, promotion of unfolded protein clearance and modulation of cell survival.
31428526	1	5	theme	physiological	187:199	arg1	functions					201:209	different physiological functions	177:209	different physiological functions such as control of complement activation, promotion of unfolded protein clearance and modulation of cell survival	177:323	Clusterin is a glycoprotein able to mediate different physiological functions such as control of complement activation, promotion of unfolded protein clearance and modulation of cell survival.
31428526	1	5	theme	physiological	187:199	arg1	promotion					253:261	promotion	253:261	promotion of unfolded protein clearance	253:291	Clusterin is a glycoprotein able to mediate different physiological functions such as control of complement activation, promotion of unfolded protein clearance and modulation of cell survival.
31428526	1	5	theme	physiological	187:199	arg1	modulation					297:306	modulation	297:306	modulation of cell survival	297:323	Clusterin is a glycoprotein able to mediate different physiological functions such as control of complement activation, promotion of unfolded protein clearance and modulation of cell survival.
31428526	1	5	theme	physiological	187:199	arg1	control					219:225	control	219:225	control of complement activation	219:250	Clusterin is a glycoprotein able to mediate different physiological functions such as control of complement activation, promotion of unfolded protein clearance and modulation of cell survival.
31428526	8	6	gly	fucosylated	1498:1508	arg1	clusterin					1510:1518	fucosylated clusterin	1498:1518	fucosylated clusterin produced by luminal breast cancer cells	1498:1558	Our results reveal an unexpected complexity on the structure and function of secretory clusterin produced by tumors and suggest that fucosylated clusterin produced by luminal breast cancer cells might play a role in tumor progression by promoting the release of pro-angiogenic factors by intratumoral macrophages.
31428526	7	7	link	derived	1191:1197	arg1	macrophages					1199:1209	monocyte derived macrophages	1182:1209	monocyte derived macrophages	1182:1209	Moreover, experiments performed in vitro using semen fucosylated clusterin and monocyte derived macrophages showed that the interaction of semen clusterin with DC-SIGN promoted a proangiogenic profile, characterized by a high production of VEGF, IL-8 and TNF-α.
31428526	4	8	theme	human	631:635	arg1	clusterin					665:673	human luminal breast cancer (LBC) clusterin	631:673	human luminal breast cancer (LBC) clusterin	631:673	Here we show that human luminal breast cancer (LBC) clusterin also bears terminal fucosylated glycans, conferring clusterin the ability to interact with DC-SIGN, a C-type lectin receptor expressed by myeloid cells.
31428526	7	9	with	interaction	1227:1237	arg1	DC-SIGN					1263:1269	DC-SIGN	1263:1269	DC-SIGN	1263:1269	Moreover, experiments performed in vitro using semen fucosylated clusterin and monocyte derived macrophages showed that the interaction of semen clusterin with DC-SIGN promoted a proangiogenic profile, characterized by a high production of VEGF, IL-8 and TNF-α.
31428526	6	10	theme	intratumoral	1077:1088	arg1	macrophages					1090:1100	luminal breast cancer intratumoral macrophages	1055:1100	luminal breast cancer intratumoral macrophages	1055:1100	We also found that DC-SIGN is expressed by luminal breast cancer intratumoral macrophages.
31428526	4	11	theme	breast	645:650	arg1	LBC					660:662	LBC	660:662	LBC	660:662	Here we show that human luminal breast cancer (LBC) clusterin also bears terminal fucosylated glycans, conferring clusterin the ability to interact with DC-SIGN, a C-type lectin receptor expressed by myeloid cells.
31428526	4	11	theme	breast	645:650	arg1	cancer					652:657	luminal breast cancer	637:657	human luminal breast cancer (LBC) clusterin	631:673	Here we show that human luminal breast cancer (LBC) clusterin also bears terminal fucosylated glycans, conferring clusterin the ability to interact with DC-SIGN, a C-type lectin receptor expressed by myeloid cells.
31428526	5	12	theme	glycosylation	843:855	arg1	pattern					857:863	This clusterin glycosylation pattern	828:863	This clusterin glycosylation pattern	828:863	This clusterin glycosylation pattern was absent or diminished in non-involved juxtatumoral tissue, suggesting that fucosylated clusterin might represent a cancer associated glycoform.
31428526	1	13	gly	glycoprotein	148:159	arg1	Clusterin					133:141	Clusterin	133:141	Clusterin	133:141	Clusterin is a glycoprotein able to mediate different physiological functions such as control of complement activation, promotion of unfolded protein clearance and modulation of cell survival.
31428526	1	13	gly	glycoprotein	148:159	arg1	glycoprotein					148:159	a glycoprotein	146:159	a glycoprotein able to mediate different physiological functions such as control of complement activation, promotion of unfolded protein clearance and modulation of cell survival	146:323	Clusterin is a glycoprotein able to mediate different physiological functions such as control of complement activation, promotion of unfolded protein clearance and modulation of cell survival.
31428526	3	14	theme	terminal	589:596	arg1	motifs					605:610	terminal fucose motifs	589:610	terminal fucose motifs	589:610	We have previously described a novel clusterin glycoform present in human semen, but not in serum, highly enriched in terminal fucose motifs.
31428526	3	15	from	enriched	577:584	arg1	motifs					605:610	terminal fucose motifs	589:610	terminal fucose motifs	589:610	We have previously described a novel clusterin glycoform present in human semen, but not in serum, highly enriched in terminal fucose motifs.
31428526	8	16	dep	structure	1416:1424	arg1	the					1412:1414	the	1412:1414	the	1412:1414	Our results reveal an unexpected complexity on the structure and function of secretory clusterin produced by tumors and suggest that fucosylated clusterin produced by luminal breast cancer cells might play a role in tumor progression by promoting the release of pro-angiogenic factors by intratumoral macrophages.
31428526	1	17	theme	able	161:164	arg1	Clusterin					133:141	Clusterin	133:141	Clusterin	133:141	Clusterin is a glycoprotein able to mediate different physiological functions such as control of complement activation, promotion of unfolded protein clearance and modulation of cell survival.
31428526	1	17	theme	able	161:164	arg1	glycoprotein					148:159	a glycoprotein	146:159	a glycoprotein able to mediate different physiological functions such as control of complement activation, promotion of unfolded protein clearance and modulation of cell survival	146:323	Clusterin is a glycoprotein able to mediate different physiological functions such as control of complement activation, promotion of unfolded protein clearance and modulation of cell survival.
31428526	5	18	gly	glycoform	1001:1009	arg1	cancer					983:988	a cancer associated glycoform	981:1009	a cancer associated glycoform	981:1009	This clusterin glycosylation pattern was absent or diminished in non-involved juxtatumoral tissue, suggesting that fucosylated clusterin might represent a cancer associated glycoform.
31428526	8	19	theme	breast	1540:1545	arg1	cells					1554:1558	luminal breast cancer cells	1532:1558	luminal breast cancer cells	1532:1558	Our results reveal an unexpected complexity on the structure and function of secretory clusterin produced by tumors and suggest that fucosylated clusterin produced by luminal breast cancer cells might play a role in tumor progression by promoting the release of pro-angiogenic factors by intratumoral macrophages.
31428526	4	20	theme	terminal	686:693	arg1	glycans					707:713	terminal fucosylated glycans	686:713	terminal fucosylated glycans	686:713	Here we show that human luminal breast cancer (LBC) clusterin also bears terminal fucosylated glycans, conferring clusterin the ability to interact with DC-SIGN, a C-type lectin receptor expressed by myeloid cells.
31428526	0	21	theme	cell-specific	81:93	arg1	DC-SIGN					123:129	dendritic cell-specific ICAM-grabbing non-integrin (DC-SIGN)	71:130	dendritic cell-specific ICAM-grabbing non-integrin (DC-SIGN)	71:130	Aberrant fucosylation enables breast cancer clusterin to interact with dendritic cell-specific ICAM-grabbing non-integrin (DC-SIGN).
31428526	5	22	theme	juxtatumoral	906:917	arg1	tissue					919:924	non-involved juxtatumoral tissue	893:924	non-involved juxtatumoral tissue	893:924	This clusterin glycosylation pattern was absent or diminished in non-involved juxtatumoral tissue, suggesting that fucosylated clusterin might represent a cancer associated glycoform.
31428526	0	23	theme	non-integrin	109:120	arg1	DC-SIGN					123:129	dendritic cell-specific ICAM-grabbing non-integrin (DC-SIGN)	71:130	dendritic cell-specific ICAM-grabbing non-integrin (DC-SIGN)	71:130	Aberrant fucosylation enables breast cancer clusterin to interact with dendritic cell-specific ICAM-grabbing non-integrin (DC-SIGN).
31428526	5	24	theme	cancer	983:988	arg1	glycoform					1001:1009	a cancer associated glycoform	981:1009	a cancer associated glycoform	981:1009	This clusterin glycosylation pattern was absent or diminished in non-involved juxtatumoral tissue, suggesting that fucosylated clusterin might represent a cancer associated glycoform.
31428526	7	25	theme	IL-8	1349:1352	arg1	production					1329:1338	a high production	1322:1338	a high production of VEGF, IL-8 and TNF-α	1322:1362	Moreover, experiments performed in vitro using semen fucosylated clusterin and monocyte derived macrophages showed that the interaction of semen clusterin with DC-SIGN promoted a proangiogenic profile, characterized by a high production of VEGF, IL-8 and TNF-α.
31428526	8	26	theme	unexpected	1387:1396	arg1	complexity					1398:1407	an unexpected complexity	1384:1407	an unexpected complexity on the structure and function of secretory clusterin produced by tumors	1384:1479	Our results reveal an unexpected complexity on the structure and function of secretory clusterin produced by tumors and suggest that fucosylated clusterin produced by luminal breast cancer cells might play a role in tumor progression by promoting the release of pro-angiogenic factors by intratumoral macrophages.
31428526	3	27	gly	glycoform	518:526	arg1	clusterin					508:516	a novel clusterin glycoform	500:526	a novel clusterin glycoform present in human semen, but not in serum	500:567	We have previously described a novel clusterin glycoform present in human semen, but not in serum, highly enriched in terminal fucose motifs.
31428526	0	28	theme	Aberrant	0:7	arg1	fucosylation					9:20	Aberrant fucosylation	0:20	Aberrant fucosylation	0:20	Aberrant fucosylation enables breast cancer clusterin to interact with dendritic cell-specific ICAM-grabbing non-integrin (DC-SIGN).
31428526	7	29	theme	TNF-α	1358:1362	arg1	production					1329:1338	a high production	1322:1338	a high production of VEGF, IL-8 and TNF-α	1322:1362	Moreover, experiments performed in vitro using semen fucosylated clusterin and monocyte derived macrophages showed that the interaction of semen clusterin with DC-SIGN promoted a proangiogenic profile, characterized by a high production of VEGF, IL-8 and TNF-α.
31428526	2	30	theme	tumor	452:456	arg1	progression					458:468	tumor progression	452:468	tumor progression	452:468	Clusterin is overexpressed in many types of cancers and a large body of evidence suggests that it promotes carcinogenesis and tumor progression.
31428526	8	31	theme	tumor	1581:1585	arg1	progression					1587:1597	tumor progression	1581:1597	tumor progression	1581:1597	Our results reveal an unexpected complexity on the structure and function of secretory clusterin produced by tumors and suggest that fucosylated clusterin produced by luminal breast cancer cells might play a role in tumor progression by promoting the release of pro-angiogenic factors by intratumoral macrophages.
31428526	1	32	theme	cell	311:314	arg1	survival					316:323	cell survival	311:323	cell survival	311:323	Clusterin is a glycoprotein able to mediate different physiological functions such as control of complement activation, promotion of unfolded protein clearance and modulation of cell survival.
31428526	6	33	theme	breast	1063:1068	arg1	macrophages					1090:1100	luminal breast cancer intratumoral macrophages	1055:1100	luminal breast cancer intratumoral macrophages	1055:1100	We also found that DC-SIGN is expressed by luminal breast cancer intratumoral macrophages.
31428526	3	34	from	motifs	605:610	arg1	enriched					577:584	enriched	577:584	enriched	577:584	We have previously described a novel clusterin glycoform present in human semen, but not in serum, highly enriched in terminal fucose motifs.
31428526	7	35	theme	derived	1191:1197	arg1	macrophages					1199:1209	monocyte derived macrophages	1182:1209	monocyte derived macrophages	1182:1209	Moreover, experiments performed in vitro using semen fucosylated clusterin and monocyte derived macrophages showed that the interaction of semen clusterin with DC-SIGN promoted a proangiogenic profile, characterized by a high production of VEGF, IL-8 and TNF-α.
31428526	7	36	theme	VEGF	1343:1346	arg1	production					1329:1338	a high production	1322:1338	a high production of VEGF, IL-8 and TNF-α	1322:1362	Moreover, experiments performed in vitro using semen fucosylated clusterin and monocyte derived macrophages showed that the interaction of semen clusterin with DC-SIGN promoted a proangiogenic profile, characterized by a high production of VEGF, IL-8 and TNF-α.
31428526	8	37	theme	secretory	1442:1450	arg1	clusterin					1452:1460	secretory clusterin	1442:1460	secretory clusterin produced by tumors	1442:1479	Our results reveal an unexpected complexity on the structure and function of secretory clusterin produced by tumors and suggest that fucosylated clusterin produced by luminal breast cancer cells might play a role in tumor progression by promoting the release of pro-angiogenic factors by intratumoral macrophages.
31428526	3	38	theme	clusterin	508:516	arg1	glycoform					518:526	a novel clusterin glycoform	500:526	a novel clusterin glycoform present in human semen, but not in serum	500:567	We have previously described a novel clusterin glycoform present in human semen, but not in serum, highly enriched in terminal fucose motifs.
31428526	4	39	theme	C-type	777:782	arg1	receptor					791:798	a C-type lectin receptor	775:798	a C-type lectin receptor expressed by myeloid cells	775:825	Here we show that human luminal breast cancer (LBC) clusterin also bears terminal fucosylated glycans, conferring clusterin the ability to interact with DC-SIGN, a C-type lectin receptor expressed by myeloid cells.
31428526	4	39	theme	C-type	777:782	arg1	DC-SIGN					766:772	DC-SIGN	766:772	DC-SIGN	766:772	Here we show that human luminal breast cancer (LBC) clusterin also bears terminal fucosylated glycans, conferring clusterin the ability to interact with DC-SIGN, a C-type lectin receptor expressed by myeloid cells.
31428526	2	40	theme	many	356:359	arg1	types					361:365	many types	356:365	many types of cancers	356:376	Clusterin is overexpressed in many types of cancers and a large body of evidence suggests that it promotes carcinogenesis and tumor progression.
31428526	1	41	theme	activation	241:250	arg1	promotion					253:261	promotion	253:261	promotion of unfolded protein clearance	253:291	Clusterin is a glycoprotein able to mediate different physiological functions such as control of complement activation, promotion of unfolded protein clearance and modulation of cell survival.
31428526	1	41	theme	activation	241:250	arg1	control					219:225	control	219:225	control of complement activation	219:250	Clusterin is a glycoprotein able to mediate different physiological functions such as control of complement activation, promotion of unfolded protein clearance and modulation of cell survival.
31428526	1	41	theme	activation	241:250	arg1	modulation					297:306	modulation	297:306	modulation of cell survival	297:323	Clusterin is a glycoprotein able to mediate different physiological functions such as control of complement activation, promotion of unfolded protein clearance and modulation of cell survival.
31428526	8	42	theme	factors	1642:1648	arg1	release					1616:1622	the release	1612:1622	the release of pro-angiogenic factors by intratumoral macrophages	1612:1676	Our results reveal an unexpected complexity on the structure and function of secretory clusterin produced by tumors and suggest that fucosylated clusterin produced by luminal breast cancer cells might play a role in tumor progression by promoting the release of pro-angiogenic factors by intratumoral macrophages.
31428526	3	43	theme	human	539:543	arg1	semen					545:549	human semen	539:549	human semen	539:549	We have previously described a novel clusterin glycoform present in human semen, but not in serum, highly enriched in terminal fucose motifs.
31428526	8	44	theme	intratumoral	1653:1664	arg1	macrophages					1666:1676	intratumoral macrophages	1653:1676	intratumoral macrophages	1653:1676	Our results reveal an unexpected complexity on the structure and function of secretory clusterin produced by tumors and suggest that fucosylated clusterin produced by luminal breast cancer cells might play a role in tumor progression by promoting the release of pro-angiogenic factors by intratumoral macrophages.
31428526	7	45	theme	semen	1150:1154	arg1	clusterin					1168:1176	semen fucosylated clusterin	1150:1176	semen fucosylated clusterin	1150:1176	Moreover, experiments performed in vitro using semen fucosylated clusterin and monocyte derived macrophages showed that the interaction of semen clusterin with DC-SIGN promoted a proangiogenic profile, characterized by a high production of VEGF, IL-8 and TNF-α.
31428526	3	46	theme	present	528:534	arg1	glycoform					518:526	a novel clusterin glycoform	500:526	a novel clusterin glycoform present in human semen, but not in serum	500:567	We have previously described a novel clusterin glycoform present in human semen, but not in serum, highly enriched in terminal fucose motifs.
31428526	1	47	theme	unfolded	266:273	arg1	clearance					283:291	unfolded protein clearance	266:291	unfolded protein clearance	266:291	Clusterin is a glycoprotein able to mediate different physiological functions such as control of complement activation, promotion of unfolded protein clearance and modulation of cell survival.
31428526	8	48	from	complexity	1398:1407	arg1	function					1430:1437	function	1430:1437	function	1430:1437	Our results reveal an unexpected complexity on the structure and function of secretory clusterin produced by tumors and suggest that fucosylated clusterin produced by luminal breast cancer cells might play a role in tumor progression by promoting the release of pro-angiogenic factors by intratumoral macrophages.
31428526	8	48	from	complexity	1398:1407	arg1	structure					1416:1424	structure	1416:1424	structure	1416:1424	Our results reveal an unexpected complexity on the structure and function of secretory clusterin produced by tumors and suggest that fucosylated clusterin produced by luminal breast cancer cells might play a role in tumor progression by promoting the release of pro-angiogenic factors by intratumoral macrophages.
31428526	1	49	theme	clearance	283:291	arg1	promotion					253:261	promotion	253:261	promotion of unfolded protein clearance	253:291	Clusterin is a glycoprotein able to mediate different physiological functions such as control of complement activation, promotion of unfolded protein clearance and modulation of cell survival.
31428526	1	49	theme	clearance	283:291	arg1	control					219:225	control	219:225	control of complement activation	219:250	Clusterin is a glycoprotein able to mediate different physiological functions such as control of complement activation, promotion of unfolded protein clearance and modulation of cell survival.
31428526	1	49	theme	clearance	283:291	arg1	modulation					297:306	modulation	297:306	modulation of cell survival	297:323	Clusterin is a glycoprotein able to mediate different physiological functions such as control of complement activation, promotion of unfolded protein clearance and modulation of cell survival.
31428526	3	50	from	semen	545:549	arg1	present					528:534	present	528:534	present	528:534	We have previously described a novel clusterin glycoform present in human semen, but not in serum, highly enriched in terminal fucose motifs.
31428526	1	51	theme	different	177:185	arg1	functions					201:209	different physiological functions	177:209	different physiological functions such as control of complement activation, promotion of unfolded protein clearance and modulation of cell survival	177:323	Clusterin is a glycoprotein able to mediate different physiological functions such as control of complement activation, promotion of unfolded protein clearance and modulation of cell survival.
31428526	1	51	theme	different	177:185	arg1	promotion					253:261	promotion	253:261	promotion of unfolded protein clearance	253:291	Clusterin is a glycoprotein able to mediate different physiological functions such as control of complement activation, promotion of unfolded protein clearance and modulation of cell survival.
31428526	1	51	theme	different	177:185	arg1	modulation					297:306	modulation	297:306	modulation of cell survival	297:323	Clusterin is a glycoprotein able to mediate different physiological functions such as control of complement activation, promotion of unfolded protein clearance and modulation of cell survival.
31428526	1	51	theme	different	177:185	arg1	control					219:225	control	219:225	control of complement activation	219:250	Clusterin is a glycoprotein able to mediate different physiological functions such as control of complement activation, promotion of unfolded protein clearance and modulation of cell survival.
31428526	4	52	theme	cancer	652:657	arg1	clusterin					665:673	human luminal breast cancer (LBC) clusterin	631:673	human luminal breast cancer (LBC) clusterin	631:673	Here we show that human luminal breast cancer (LBC) clusterin also bears terminal fucosylated glycans, conferring clusterin the ability to interact with DC-SIGN, a C-type lectin receptor expressed by myeloid cells.
31428526	4	53	gly	fucosylated	695:705	arg1	glycans					707:713	terminal fucosylated glycans	686:713	terminal fucosylated glycans	686:713	Here we show that human luminal breast cancer (LBC) clusterin also bears terminal fucosylated glycans, conferring clusterin the ability to interact with DC-SIGN, a C-type lectin receptor expressed by myeloid cells.
31428526	3	54	theme	fucose	598:603	arg1	motifs					605:610	terminal fucose motifs	589:610	terminal fucose motifs	589:610	We have previously described a novel clusterin glycoform present in human semen, but not in serum, highly enriched in terminal fucose motifs.
31428526	5	55	theme	clusterin	833:841	arg1	pattern					857:863	This clusterin glycosylation pattern	828:863	This clusterin glycosylation pattern	828:863	This clusterin glycosylation pattern was absent or diminished in non-involved juxtatumoral tissue, suggesting that fucosylated clusterin might represent a cancer associated glycoform.
31428526	8	56	theme	fucosylated	1498:1508	arg1	clusterin					1510:1518	fucosylated clusterin	1498:1518	fucosylated clusterin produced by luminal breast cancer cells	1498:1558	Our results reveal an unexpected complexity on the structure and function of secretory clusterin produced by tumors and suggest that fucosylated clusterin produced by luminal breast cancer cells might play a role in tumor progression by promoting the release of pro-angiogenic factors by intratumoral macrophages.
31428526	4	57	theme	luminal	637:643	arg1	LBC					660:662	LBC	660:662	LBC	660:662	Here we show that human luminal breast cancer (LBC) clusterin also bears terminal fucosylated glycans, conferring clusterin the ability to interact with DC-SIGN, a C-type lectin receptor expressed by myeloid cells.
31428526	4	57	theme	luminal	637:643	arg1	cancer					652:657	luminal breast cancer	637:657	human luminal breast cancer (LBC) clusterin	631:673	Here we show that human luminal breast cancer (LBC) clusterin also bears terminal fucosylated glycans, conferring clusterin the ability to interact with DC-SIGN, a C-type lectin receptor expressed by myeloid cells.
31428526	4	58	theme	fucosylated	695:705	arg1	glycans					707:713	terminal fucosylated glycans	686:713	terminal fucosylated glycans	686:713	Here we show that human luminal breast cancer (LBC) clusterin also bears terminal fucosylated glycans, conferring clusterin the ability to interact with DC-SIGN, a C-type lectin receptor expressed by myeloid cells.
31428526	3	59	from	serum	563:567	arg1	present					528:534	present	528:534	present	528:534	We have previously described a novel clusterin glycoform present in human semen, but not in serum, highly enriched in terminal fucose motifs.
31428526	8	60	theme	luminal	1532:1538	arg1	cells					1554:1558	luminal breast cancer cells	1532:1558	luminal breast cancer cells	1532:1558	Our results reveal an unexpected complexity on the structure and function of secretory clusterin produced by tumors and suggest that fucosylated clusterin produced by luminal breast cancer cells might play a role in tumor progression by promoting the release of pro-angiogenic factors by intratumoral macrophages.
31428526	0	61	theme	dendritic	71:79	arg1	DC-SIGN					123:129	dendritic cell-specific ICAM-grabbing non-integrin (DC-SIGN)	71:130	dendritic cell-specific ICAM-grabbing non-integrin (DC-SIGN)	71:130	Aberrant fucosylation enables breast cancer clusterin to interact with dendritic cell-specific ICAM-grabbing non-integrin (DC-SIGN).
31428526	7	62	gly	fucosylated	1156:1166	arg1	clusterin					1168:1176	semen fucosylated clusterin	1150:1176	semen fucosylated clusterin	1150:1176	Moreover, experiments performed in vitro using semen fucosylated clusterin and monocyte derived macrophages showed that the interaction of semen clusterin with DC-SIGN promoted a proangiogenic profile, characterized by a high production of VEGF, IL-8 and TNF-α.
31428526	8	63	theme	cancer	1547:1552	arg1	cells					1554:1558	luminal breast cancer cells	1532:1558	luminal breast cancer cells	1532:1558	Our results reveal an unexpected complexity on the structure and function of secretory clusterin produced by tumors and suggest that fucosylated clusterin produced by luminal breast cancer cells might play a role in tumor progression by promoting the release of pro-angiogenic factors by intratumoral macrophages.
31428526	7	64	theme	clusterin	1248:1256	arg1	interaction					1227:1237	the interaction	1223:1237	the interaction of semen clusterin with DC-SIGN	1223:1269	Moreover, experiments performed in vitro using semen fucosylated clusterin and monocyte derived macrophages showed that the interaction of semen clusterin with DC-SIGN promoted a proangiogenic profile, characterized by a high production of VEGF, IL-8 and TNF-α.
31428526	0	65	theme	ICAM-grabbing	95:107	arg1	DC-SIGN					123:129	dendritic cell-specific ICAM-grabbing non-integrin (DC-SIGN)	71:130	dendritic cell-specific ICAM-grabbing non-integrin (DC-SIGN)	71:130	Aberrant fucosylation enables breast cancer clusterin to interact with dendritic cell-specific ICAM-grabbing non-integrin (DC-SIGN).
31428526	5	66	theme	non-involved	893:904	arg1	tissue					919:924	non-involved juxtatumoral tissue	893:924	non-involved juxtatumoral tissue	893:924	This clusterin glycosylation pattern was absent or diminished in non-involved juxtatumoral tissue, suggesting that fucosylated clusterin might represent a cancer associated glycoform.
31428526	7	67	theme	semen	1242:1246	arg1	clusterin					1248:1256	semen clusterin	1242:1256	semen clusterin	1242:1256	Moreover, experiments performed in vitro using semen fucosylated clusterin and monocyte derived macrophages showed that the interaction of semen clusterin with DC-SIGN promoted a proangiogenic profile, characterized by a high production of VEGF, IL-8 and TNF-α.
31428526	5	68	gly	fucosylated	943:953	arg1	clusterin					955:963	fucosylated clusterin	943:963	fucosylated clusterin	943:963	This clusterin glycosylation pattern was absent or diminished in non-involved juxtatumoral tissue, suggesting that fucosylated clusterin might represent a cancer associated glycoform.
31428526	5	69	theme	associated	990:999	arg1	glycoform					1001:1009	a cancer associated glycoform	981:1009	a cancer associated glycoform	981:1009	This clusterin glycosylation pattern was absent or diminished in non-involved juxtatumoral tissue, suggesting that fucosylated clusterin might represent a cancer associated glycoform.
31428526	2	70	theme	evidence	398:405	arg1	body					390:393	a large body	382:393	a large body of evidence	382:405	Clusterin is overexpressed in many types of cancers and a large body of evidence suggests that it promotes carcinogenesis and tumor progression.
31428526	5	71	theme	fucosylated	943:953	arg1	clusterin					955:963	fucosylated clusterin	943:963	fucosylated clusterin	943:963	This clusterin glycosylation pattern was absent or diminished in non-involved juxtatumoral tissue, suggesting that fucosylated clusterin might represent a cancer associated glycoform.
31428526	7	72	dep	derived	1191:1197	arg1	monocyte					1182:1189	monocyte	1182:1189	monocyte	1182:1189	Moreover, experiments performed in vitro using semen fucosylated clusterin and monocyte derived macrophages showed that the interaction of semen clusterin with DC-SIGN promoted a proangiogenic profile, characterized by a high production of VEGF, IL-8 and TNF-α.
31428526	0	73	theme	breast	30:35	arg1	cancer					37:42	breast cancer	30:42	breast cancer	30:42	Aberrant fucosylation enables breast cancer clusterin to interact with dendritic cell-specific ICAM-grabbing non-integrin (DC-SIGN).
31428526	6	74	theme	cancer	1070:1075	arg1	macrophages					1090:1100	luminal breast cancer intratumoral macrophages	1055:1100	luminal breast cancer intratumoral macrophages	1055:1100	We also found that DC-SIGN is expressed by luminal breast cancer intratumoral macrophages.
31428526	2	75	theme	large	384:388	arg1	body					390:393	a large body	382:393	a large body of evidence	382:405	Clusterin is overexpressed in many types of cancers and a large body of evidence suggests that it promotes carcinogenesis and tumor progression.
31428526	1	76	theme	survival	316:323	arg1	promotion					253:261	promotion	253:261	promotion of unfolded protein clearance	253:291	Clusterin is a glycoprotein able to mediate different physiological functions such as control of complement activation, promotion of unfolded protein clearance and modulation of cell survival.
31428526	1	76	theme	survival	316:323	arg1	control					219:225	control	219:225	control of complement activation	219:250	Clusterin is a glycoprotein able to mediate different physiological functions such as control of complement activation, promotion of unfolded protein clearance and modulation of cell survival.
31428526	1	76	theme	survival	316:323	arg1	modulation					297:306	modulation	297:306	modulation of cell survival	297:323	Clusterin is a glycoprotein able to mediate different physiological functions such as control of complement activation, promotion of unfolded protein clearance and modulation of cell survival.
31428526	6	77	theme	luminal	1055:1061	arg1	macrophages					1090:1100	luminal breast cancer intratumoral macrophages	1055:1100	luminal breast cancer intratumoral macrophages	1055:1100	We also found that DC-SIGN is expressed by luminal breast cancer intratumoral macrophages.
31428526	7	78	theme	high	1324:1327	arg1	production					1329:1338	a high production	1322:1338	a high production of VEGF, IL-8 and TNF-α	1322:1362	Moreover, experiments performed in vitro using semen fucosylated clusterin and monocyte derived macrophages showed that the interaction of semen clusterin with DC-SIGN promoted a proangiogenic profile, characterized by a high production of VEGF, IL-8 and TNF-α.
31428526	8	79	theme	pro-angiogenic	1627:1640	arg1	factors					1642:1648	pro-angiogenic factors	1627:1648	pro-angiogenic factors	1627:1648	Our results reveal an unexpected complexity on the structure and function of secretory clusterin produced by tumors and suggest that fucosylated clusterin produced by luminal breast cancer cells might play a role in tumor progression by promoting the release of pro-angiogenic factors by intratumoral macrophages.
31428526	4	80	theme	myeloid	813:819	arg1	cells					821:825	myeloid cells	813:825	myeloid cells	813:825	Here we show that human luminal breast cancer (LBC) clusterin also bears terminal fucosylated glycans, conferring clusterin the ability to interact with DC-SIGN, a C-type lectin receptor expressed by myeloid cells.
31428526	3	81	attach	present	528:534	arg2	glycoform					518:526	a novel clusterin glycoform	500:526	a novel clusterin glycoform present in human semen, but not in serum	500:567	We have previously described a novel clusterin glycoform present in human semen, but not in serum, highly enriched in terminal fucose motifs.
31428526	3	81	attach	present	528:534	arg1	serum					563:567	serum	563:567	serum	563:567	We have previously described a novel clusterin glycoform present in human semen, but not in serum, highly enriched in terminal fucose motifs.
31428526	3	81	attach	present	528:534	arg1	semen					545:549	human semen	539:549	human semen	539:549	We have previously described a novel clusterin glycoform present in human semen, but not in serum, highly enriched in terminal fucose motifs.
31428526	8	82	theme	clusterin	1452:1460	arg1	function					1430:1437	function	1430:1437	function	1430:1437	Our results reveal an unexpected complexity on the structure and function of secretory clusterin produced by tumors and suggest that fucosylated clusterin produced by luminal breast cancer cells might play a role in tumor progression by promoting the release of pro-angiogenic factors by intratumoral macrophages.
31428526	8	82	theme	clusterin	1452:1460	arg1	structure					1416:1424	structure	1416:1424	structure	1416:1424	Our results reveal an unexpected complexity on the structure and function of secretory clusterin produced by tumors and suggest that fucosylated clusterin produced by luminal breast cancer cells might play a role in tumor progression by promoting the release of pro-angiogenic factors by intratumoral macrophages.
31428526	3	83	theme	novel	502:506	arg1	glycoform					518:526	a novel clusterin glycoform	500:526	a novel clusterin glycoform present in human semen, but not in serum	500:567	We have previously described a novel clusterin glycoform present in human semen, but not in serum, highly enriched in terminal fucose motifs.
31428526	1	84	theme	complement	230:239	arg1	activation					241:250	complement activation	230:250	complement activation	230:250	Clusterin is a glycoprotein able to mediate different physiological functions such as control of complement activation, promotion of unfolded protein clearance and modulation of cell survival.
31428526	4	85	contain	bears	680:684	arg1	clusterin					665:673	human luminal breast cancer (LBC) clusterin	631:673	human luminal breast cancer (LBC) clusterin	631:673	Here we show that human luminal breast cancer (LBC) clusterin also bears terminal fucosylated glycans, conferring clusterin the ability to interact with DC-SIGN, a C-type lectin receptor expressed by myeloid cells.
31428526	4	85	contain	bears	680:684	arg2	glycans					707:713	terminal fucosylated glycans	686:713	terminal fucosylated glycans	686:713	Here we show that human luminal breast cancer (LBC) clusterin also bears terminal fucosylated glycans, conferring clusterin the ability to interact with DC-SIGN, a C-type lectin receptor expressed by myeloid cells.
31428526	4	86	theme	lectin	784:789	arg1	receptor					791:798	a C-type lectin receptor	775:798	a C-type lectin receptor expressed by myeloid cells	775:825	Here we show that human luminal breast cancer (LBC) clusterin also bears terminal fucosylated glycans, conferring clusterin the ability to interact with DC-SIGN, a C-type lectin receptor expressed by myeloid cells.
31428526	4	86	theme	lectin	784:789	arg1	DC-SIGN					766:772	DC-SIGN	766:772	DC-SIGN	766:772	Here we show that human luminal breast cancer (LBC) clusterin also bears terminal fucosylated glycans, conferring clusterin the ability to interact with DC-SIGN, a C-type lectin receptor expressed by myeloid cells.
31759038	0	0	theme	DSS-induced	87:97	arg1	colitis					107:113	DSS-induced chronic colitis	87:113	DSS-induced chronic colitis	87:113	Probiotic mixtures with aerobic constituent promoted the recovery of multi-barriers in DSS-induced chronic colitis.
31759038	4	1	dep	transplantation	752:766	arg1	control					788:794	a positive control	777:794	a positive control	777:794	The probiotic mixtures P-qua or P-tri was gavage administrated respectively, while fecal microbiota transplantation (FMT) as a positive control.
31759038	0	2	from	recovery	57:64	arg1	colitis					107:113	DSS-induced chronic colitis	87:113	DSS-induced chronic colitis	87:113	Probiotic mixtures with aerobic constituent promoted the recovery of multi-barriers in DSS-induced chronic colitis.
31759038	6	3	theme	Altered	984:990	arg1	composition					992:1002	Altered composition	984:1002	Altered composition	984:1002	Altered composition and diversity in gut microbiota were observed via sequencing analysis.
31759038	7	4	theme	mucous	1222:1227	arg1	layer					1229:1233	mucous layer	1222:1233	mucous layer	1222:1233	KEY FINDINGS Both P-qua and P-tri relieved the intestinal inflammation and improved the functions of multiple barriers with increased integrity of mucous layer, enhanced transepithelial electrical resistance, declined epithelial and endothelial permeability to macromolecules in DSS-colitis.
31759038	6	5	from	composition	992:1002	arg1	microbiota					1025:1034	gut microbiota	1021:1034	gut microbiota	1021:1034	Altered composition and diversity in gut microbiota were observed via sequencing analysis.
31759038	5	6	theme	mucus	889:893	arg1	barrier					895:901	the mucus barrier	885:901	the mucus barrier	885:901	The intestinal inflammation and functions of multiple barriers were assessed, including the mucus barrier, epithelial barrier and endothelial barrier known as gut-vascular barrier (GVB).
31759038	7	7	theme	increased	1199:1207	arg1	integrity					1209:1217	increased integrity	1199:1217	increased integrity of mucous layer	1199:1233	KEY FINDINGS Both P-qua and P-tri relieved the intestinal inflammation and improved the functions of multiple barriers with increased integrity of mucous layer, enhanced transepithelial electrical resistance, declined epithelial and endothelial permeability to macromolecules in DSS-colitis.
31759038	2	8	theme	therapeutic	282:292	arg1	roles					294:298	the therapeutic roles	278:298	the therapeutic roles of probiotic mixtures of Bifidobacterium infantis, Lactobacillus acidophilus, Enterococcus faecalis with (quadruple probiotics, P-qua) or without (triple probiotics, P-tri) aerobic	278:479	This study aimed to explore the therapeutic roles of probiotic mixtures of Bifidobacterium infantis, Lactobacillus acidophilus, Enterococcus faecalis with (quadruple probiotics, P-qua) or without (triple probiotics, P-tri) aerobic Bacillus cereus in colitis, focusing on the multiple barrier functions.
31759038	3	9	theme	MATERIALS	553:561	arg1	colitis					583:589	MATERIALS AND METHODS Chronic colitis	553:589	MATERIALS AND METHODS Chronic colitis	553:589	MATERIALS AND METHODS Chronic colitis was induced by dextran sulfate sodium (DSS) in C57BL/6 mice.
31759038	0	10	theme	chronic	99:105	arg1	colitis					107:113	DSS-induced chronic colitis	87:113	DSS-induced chronic colitis	87:113	Probiotic mixtures with aerobic constituent promoted the recovery of multi-barriers in DSS-induced chronic colitis.
31759038	1	11	attach	linked	153:158	arg2	target					214:219	a reliable target	203:219	a reliable target for intestinal inflammation	203:247	AIMS Gut microbiota has been closely linked to the mucosal immune and been regarded as a reliable target for intestinal inflammation.
31759038	1	11	attach	linked	153:158	arg1	immune					175:180	immune	175:180	immune	175:180	AIMS Gut microbiota has been closely linked to the mucosal immune and been regarded as a reliable target for intestinal inflammation.
31759038	1	11	attach	linked	153:158	arg2	microbiota					125:134	AIMS Gut microbiota	116:134	AIMS Gut microbiota	116:134	AIMS Gut microbiota has been closely linked to the mucosal immune and been regarded as a reliable target for intestinal inflammation.
31759038	5	12	theme	gut-vascular	956:967	arg1	GVB					978:980	GVB	978:980	GVB	978:980	The intestinal inflammation and functions of multiple barriers were assessed, including the mucus barrier, epithelial barrier and endothelial barrier known as gut-vascular barrier (GVB).
31759038	5	12	theme	gut-vascular	956:967	arg1	barrier					969:975	gut-vascular barrier	956:975	gut-vascular barrier (GVB)	956:981	The intestinal inflammation and functions of multiple barriers were assessed, including the mucus barrier, epithelial barrier and endothelial barrier known as gut-vascular barrier (GVB).
31759038	7	13	theme	transepithelial	1245:1259	arg1	resistance					1272:1281	transepithelial electrical resistance	1245:1281	transepithelial electrical resistance	1245:1281	KEY FINDINGS Both P-qua and P-tri relieved the intestinal inflammation and improved the functions of multiple barriers with increased integrity of mucous layer, enhanced transepithelial electrical resistance, declined epithelial and endothelial permeability to macromolecules in DSS-colitis.
31759038	4	14	theme	microbiota	741:750	arg1	transplantation					752:766	fecal microbiota transplantation	735:766	fecal microbiota transplantation (FMT)	735:772	The probiotic mixtures P-qua or P-tri was gavage administrated respectively, while fecal microbiota transplantation (FMT) as a positive control.
31759038	4	14	theme	microbiota	741:750	arg1	FMT					769:771	FMT	769:771	FMT	769:771	The probiotic mixtures P-qua or P-tri was gavage administrated respectively, while fecal microbiota transplantation (FMT) as a positive control.
31759038	8	15	theme	Aerobe-contained	1367:1382	arg1	P-qua					1384:1388	Aerobe-contained P-qua	1367:1388	Aerobe-contained P-qua	1367:1388	Aerobe-contained P-qua revealed a more active role in barrier recovering relative to P-tri, while FMT as a positive control seemed to get better results than pure probiotics.
31759038	7	16	theme	barriers	1185:1192	arg1	functions					1163:1171	the functions	1159:1171	the functions of multiple barriers	1159:1192	KEY FINDINGS Both P-qua and P-tri relieved the intestinal inflammation and improved the functions of multiple barriers with increased integrity of mucous layer, enhanced transepithelial electrical resistance, declined epithelial and endothelial permeability to macromolecules in DSS-colitis.
31759038	7	17	theme	epithelial	1293:1302	arg1	permeability					1320:1331	epithelial and endothelial permeability	1293:1331	epithelial and endothelial permeability to macromolecules in DSS-colitis	1293:1364	KEY FINDINGS Both P-qua and P-tri relieved the intestinal inflammation and improved the functions of multiple barriers with increased integrity of mucous layer, enhanced transepithelial electrical resistance, declined epithelial and endothelial permeability to macromolecules in DSS-colitis.
31759038	5	18	theme	endothelial	927:937	arg1	barrier					939:945	endothelial barrier	927:945	endothelial barrier	927:945	The intestinal inflammation and functions of multiple barriers were assessed, including the mucus barrier, epithelial barrier and endothelial barrier known as gut-vascular barrier (GVB).
31759038	4	19	dep	mixtures	666:673	arg1	P-qua					675:679	P-qua	675:679	P-qua	675:679	The probiotic mixtures P-qua or P-tri was gavage administrated respectively, while fecal microbiota transplantation (FMT) as a positive control.
31759038	4	19	dep	mixtures	666:673	arg1	mixtures					666:673	The probiotic mixtures	652:673	The probiotic mixtures P-qua or P-tri	652:688	The probiotic mixtures P-qua or P-tri was gavage administrated respectively, while fecal microbiota transplantation (FMT) as a positive control.
31759038	4	19	dep	mixtures	666:673	arg1	P-tri					684:688	P-tri	684:688	P-tri	684:688	The probiotic mixtures P-qua or P-tri was gavage administrated respectively, while fecal microbiota transplantation (FMT) as a positive control.
31759038	2	20	theme	quadruple	406:414	arg1	P-qua					428:432	P-qua	428:432	P-qua	428:432	This study aimed to explore the therapeutic roles of probiotic mixtures of Bifidobacterium infantis, Lactobacillus acidophilus, Enterococcus faecalis with (quadruple probiotics, P-qua) or without (triple probiotics, P-tri) aerobic Bacillus cereus in colitis, focusing on the multiple barrier functions.
31759038	2	20	theme	quadruple	406:414	arg1	probiotics					416:425	quadruple probiotics	406:425	quadruple probiotics	406:425	This study aimed to explore the therapeutic roles of probiotic mixtures of Bifidobacterium infantis, Lactobacillus acidophilus, Enterococcus faecalis with (quadruple probiotics, P-qua) or without (triple probiotics, P-tri) aerobic Bacillus cereus in colitis, focusing on the multiple barrier functions.
31759038	7	21	theme	multiple	1176:1183	arg1	barriers					1185:1192	multiple barriers	1176:1192	multiple barriers	1176:1192	KEY FINDINGS Both P-qua and P-tri relieved the intestinal inflammation and improved the functions of multiple barriers with increased integrity of mucous layer, enhanced transepithelial electrical resistance, declined epithelial and endothelial permeability to macromolecules in DSS-colitis.
31759038	5	22	theme	barriers	851:858	arg1	functions					829:837	functions	829:837	functions	829:837	The intestinal inflammation and functions of multiple barriers were assessed, including the mucus barrier, epithelial barrier and endothelial barrier known as gut-vascular barrier (GVB).
31759038	5	22	theme	barriers	851:858	arg1	inflammation					812:823	intestinal inflammation	801:823	intestinal inflammation	801:823	The intestinal inflammation and functions of multiple barriers were assessed, including the mucus barrier, epithelial barrier and endothelial barrier known as gut-vascular barrier (GVB).
31759038	2	23	theme	triple	447:452	arg1	P-tri					466:470	P-tri	466:470	P-tri	466:470	This study aimed to explore the therapeutic roles of probiotic mixtures of Bifidobacterium infantis, Lactobacillus acidophilus, Enterococcus faecalis with (quadruple probiotics, P-qua) or without (triple probiotics, P-tri) aerobic Bacillus cereus in colitis, focusing on the multiple barrier functions.
31759038	2	23	theme	triple	447:452	arg1	probiotics					454:463	triple probiotics	447:463	triple probiotics	447:463	This study aimed to explore the therapeutic roles of probiotic mixtures of Bifidobacterium infantis, Lactobacillus acidophilus, Enterococcus faecalis with (quadruple probiotics, P-qua) or without (triple probiotics, P-tri) aerobic Bacillus cereus in colitis, focusing on the multiple barrier functions.
31759038	7	24	theme	KEY	1075:1077	arg1	FINDINGS					1079:1086	KEY FINDINGS	1075:1086	KEY FINDINGS	1075:1086	KEY FINDINGS Both P-qua and P-tri relieved the intestinal inflammation and improved the functions of multiple barriers with increased integrity of mucous layer, enhanced transepithelial electrical resistance, declined epithelial and endothelial permeability to macromolecules in DSS-colitis.
31759038	7	25	theme	layer	1229:1233	arg1	integrity					1209:1217	increased integrity	1199:1217	increased integrity of mucous layer	1199:1233	KEY FINDINGS Both P-qua and P-tri relieved the intestinal inflammation and improved the functions of multiple barriers with increased integrity of mucous layer, enhanced transepithelial electrical resistance, declined epithelial and endothelial permeability to macromolecules in DSS-colitis.
31759038	2	26	theme	mixtures	313:320	arg1	roles					294:298	the therapeutic roles	278:298	the therapeutic roles of probiotic mixtures of Bifidobacterium infantis, Lactobacillus acidophilus, Enterococcus faecalis with (quadruple probiotics, P-qua) or without (triple probiotics, P-tri) aerobic	278:479	This study aimed to explore the therapeutic roles of probiotic mixtures of Bifidobacterium infantis, Lactobacillus acidophilus, Enterococcus faecalis with (quadruple probiotics, P-qua) or without (triple probiotics, P-tri) aerobic Bacillus cereus in colitis, focusing on the multiple barrier functions.
31759038	3	27	from	sodium	622:627	arg1	mice					646:649	C57BL/6 mice	638:649	C57BL/6 mice	638:649	MATERIALS AND METHODS Chronic colitis was induced by dextran sulfate sodium (DSS) in C57BL/6 mice.
31759038	6	28	theme	sequencing	1054:1063	arg1	analysis					1065:1072	sequencing analysis	1054:1072	sequencing analysis	1054:1072	Altered composition and diversity in gut microbiota were observed via sequencing analysis.
31759038	0	29	theme	Probiotic	0:8	arg1	mixtures					10:17	Probiotic mixtures	0:17	Probiotic mixtures with aerobic constituent	0:42	Probiotic mixtures with aerobic constituent promoted the recovery of multi-barriers in DSS-induced chronic colitis.
31759038	8	30	theme	better	1505:1510	arg1	results					1512:1518	better results	1505:1518	better results than pure probiotics	1505:1539	Aerobe-contained P-qua revealed a more active role in barrier recovering relative to P-tri, while FMT as a positive control seemed to get better results than pure probiotics.
31759038	2	31	theme	probiotic	303:311	arg1	mixtures					313:320	probiotic mixtures	303:320	probiotic mixtures of Bifidobacterium infantis, Lactobacillus acidophilus, Enterococcus faecalis with (quadruple probiotics, P-qua)	303:433	This study aimed to explore the therapeutic roles of probiotic mixtures of Bifidobacterium infantis, Lactobacillus acidophilus, Enterococcus faecalis with (quadruple probiotics, P-qua) or without (triple probiotics, P-tri) aerobic Bacillus cereus in colitis, focusing on the multiple barrier functions.
31759038	10	32	from	multi-barriers	1884:1897	arg1	gut					1915:1917	the inflamed gut	1902:1917	the inflamed gut	1902:1917	SIGNIFICANCE Aerobe-contained P-qua was a powerful adjuvant therapy for chronic colitis, via restoring the intestinal microflora and recovering the multi-barriers in the inflamed gut.
31759038	9	33	theme	Bifidobacterium	1675:1689	arg1	DSS-colitis					1628:1638	DSS-colitis	1628:1638	DSS-colitis	1628:1638	Indeed, P-qua was effective in rebuilding the structure and diversity of gut flora in DSS-colitis, especially increased abundance of Bifidobacterium, Akkermansia, Lactobacillus and Bacteroides.
31759038	9	33	theme	Bifidobacterium	1675:1689	arg1	abundance					1662:1670	increased abundance	1652:1670	increased abundance of Bifidobacterium, Akkermansia, Lactobacillus and Bacteroides	1652:1733	Indeed, P-qua was effective in rebuilding the structure and diversity of gut flora in DSS-colitis, especially increased abundance of Bifidobacterium, Akkermansia, Lactobacillus and Bacteroides.
31759038	0	34	theme	aerobic	24:30	arg1	constituent					32:42	aerobic constituent	24:42	aerobic constituent	24:42	Probiotic mixtures with aerobic constituent promoted the recovery of multi-barriers in DSS-induced chronic colitis.
31759038	5	35	dep	inflammation	812:823	arg1	The					797:799	The	797:799	The	797:799	The intestinal inflammation and functions of multiple barriers were assessed, including the mucus barrier, epithelial barrier and endothelial barrier known as gut-vascular barrier (GVB).
31759038	10	36	theme	intestinal	1843:1852	arg1	microflora					1854:1863	the intestinal microflora	1839:1863	the intestinal microflora	1839:1863	SIGNIFICANCE Aerobe-contained P-qua was a powerful adjuvant therapy for chronic colitis, via restoring the intestinal microflora and recovering the multi-barriers in the inflamed gut.
31759038	4	37	theme	fecal	735:739	arg1	transplantation					752:766	fecal microbiota transplantation	735:766	fecal microbiota transplantation (FMT)	735:772	The probiotic mixtures P-qua or P-tri was gavage administrated respectively, while fecal microbiota transplantation (FMT) as a positive control.
31759038	4	37	theme	fecal	735:739	arg1	FMT					769:771	FMT	769:771	FMT	769:771	The probiotic mixtures P-qua or P-tri was gavage administrated respectively, while fecal microbiota transplantation (FMT) as a positive control.
31759038	3	38	theme	METHODS	567:573	arg1	colitis					583:589	MATERIALS AND METHODS Chronic colitis	553:589	MATERIALS AND METHODS Chronic colitis	553:589	MATERIALS AND METHODS Chronic colitis was induced by dextran sulfate sodium (DSS) in C57BL/6 mice.
31759038	2	39	theme	barrier	534:540	arg1	functions					542:550	the multiple barrier functions	521:550	the multiple barrier functions	521:550	This study aimed to explore the therapeutic roles of probiotic mixtures of Bifidobacterium infantis, Lactobacillus acidophilus, Enterococcus faecalis with (quadruple probiotics, P-qua) or without (triple probiotics, P-tri) aerobic Bacillus cereus in colitis, focusing on the multiple barrier functions.
31759038	8	40	theme	positive	1474:1481	arg1	control					1483:1489	a positive control	1472:1489	a positive control	1472:1489	Aerobe-contained P-qua revealed a more active role in barrier recovering relative to P-tri, while FMT as a positive control seemed to get better results than pure probiotics.
31759038	1	41	theme	AIMS	116:119	arg1	target					214:219	a reliable target	203:219	a reliable target for intestinal inflammation	203:247	AIMS Gut microbiota has been closely linked to the mucosal immune and been regarded as a reliable target for intestinal inflammation.
31759038	1	41	theme	AIMS	116:119	arg1	microbiota					125:134	AIMS Gut microbiota	116:134	AIMS Gut microbiota	116:134	AIMS Gut microbiota has been closely linked to the mucosal immune and been regarded as a reliable target for intestinal inflammation.
31759038	10	42	theme	chronic	1808:1814	arg1	colitis					1816:1822	chronic colitis	1808:1822	chronic colitis	1808:1822	SIGNIFICANCE Aerobe-contained P-qua was a powerful adjuvant therapy for chronic colitis, via restoring the intestinal microflora and recovering the multi-barriers in the inflamed gut.
31759038	10	43	theme	adjuvant	1787:1794	arg1	therapy					1796:1802	a powerful adjuvant therapy	1776:1802	a powerful adjuvant therapy for chronic colitis	1776:1822	SIGNIFICANCE Aerobe-contained P-qua was a powerful adjuvant therapy for chronic colitis, via restoring the intestinal microflora and recovering the multi-barriers in the inflamed gut.
31759038	10	43	theme	adjuvant	1787:1794	arg1	P-qua					1766:1770	SIGNIFICANCE Aerobe-contained P-qua	1736:1770	SIGNIFICANCE Aerobe-contained P-qua	1736:1770	SIGNIFICANCE Aerobe-contained P-qua was a powerful adjuvant therapy for chronic colitis, via restoring the intestinal microflora and recovering the multi-barriers in the inflamed gut.
31759038	6	44	from	diversity	1008:1016	arg1	microbiota					1025:1034	gut microbiota	1021:1034	gut microbiota	1021:1034	Altered composition and diversity in gut microbiota were observed via sequencing analysis.
31759038	10	45	theme	Aerobe-contained	1749:1764	arg1	therapy					1796:1802	a powerful adjuvant therapy	1776:1802	a powerful adjuvant therapy for chronic colitis	1776:1822	SIGNIFICANCE Aerobe-contained P-qua was a powerful adjuvant therapy for chronic colitis, via restoring the intestinal microflora and recovering the multi-barriers in the inflamed gut.
31759038	10	45	theme	Aerobe-contained	1749:1764	arg1	P-qua					1766:1770	SIGNIFICANCE Aerobe-contained P-qua	1736:1770	SIGNIFICANCE Aerobe-contained P-qua	1736:1770	SIGNIFICANCE Aerobe-contained P-qua was a powerful adjuvant therapy for chronic colitis, via restoring the intestinal microflora and recovering the multi-barriers in the inflamed gut.
31759038	2	46	theme	multiple	525:532	arg1	functions					542:550	the multiple barrier functions	521:550	the multiple barrier functions	521:550	This study aimed to explore the therapeutic roles of probiotic mixtures of Bifidobacterium infantis, Lactobacillus acidophilus, Enterococcus faecalis with (quadruple probiotics, P-qua) or without (triple probiotics, P-tri) aerobic Bacillus cereus in colitis, focusing on the multiple barrier functions.
31759038	9	47	theme	gut	1615:1617	arg1	flora					1619:1623	gut flora	1615:1623	gut flora	1615:1623	Indeed, P-qua was effective in rebuilding the structure and diversity of gut flora in DSS-colitis, especially increased abundance of Bifidobacterium, Akkermansia, Lactobacillus and Bacteroides.
31759038	7	48	theme	endothelial	1308:1318	arg1	permeability					1320:1331	epithelial and endothelial permeability	1293:1331	epithelial and endothelial permeability to macromolecules in DSS-colitis	1293:1364	KEY FINDINGS Both P-qua and P-tri relieved the intestinal inflammation and improved the functions of multiple barriers with increased integrity of mucous layer, enhanced transepithelial electrical resistance, declined epithelial and endothelial permeability to macromolecules in DSS-colitis.
31759038	5	49	theme	multiple	842:849	arg1	barriers					851:858	multiple barriers	842:858	multiple barriers	842:858	The intestinal inflammation and functions of multiple barriers were assessed, including the mucus barrier, epithelial barrier and endothelial barrier known as gut-vascular barrier (GVB).
31759038	9	50	dep	structure	1588:1596	arg1	the					1584:1586	the	1584:1586	the	1584:1586	Indeed, P-qua was effective in rebuilding the structure and diversity of gut flora in DSS-colitis, especially increased abundance of Bifidobacterium, Akkermansia, Lactobacillus and Bacteroides.
31759038	7	51	dep	relieved	1109:1116	arg1	declined					1284:1291	declined	1284:1291	declined epithelial and endothelial permeability to macromolecules in DSS-colitis	1284:1364	KEY FINDINGS Both P-qua and P-tri relieved the intestinal inflammation and improved the functions of multiple barriers with increased integrity of mucous layer, enhanced transepithelial electrical resistance, declined epithelial and endothelial permeability to macromolecules in DSS-colitis.
31759038	7	51	dep	relieved	1109:1116	arg1	enhanced					1236:1243	enhanced	1236:1243	enhanced transepithelial electrical resistance	1236:1281	KEY FINDINGS Both P-qua and P-tri relieved the intestinal inflammation and improved the functions of multiple barriers with increased integrity of mucous layer, enhanced transepithelial electrical resistance, declined epithelial and endothelial permeability to macromolecules in DSS-colitis.
31759038	1	52	theme	reliable	205:212	arg1	target					214:219	a reliable target	203:219	a reliable target for intestinal inflammation	203:247	AIMS Gut microbiota has been closely linked to the mucosal immune and been regarded as a reliable target for intestinal inflammation.
31759038	1	52	theme	reliable	205:212	arg1	microbiota					125:134	AIMS Gut microbiota	116:134	AIMS Gut microbiota	116:134	AIMS Gut microbiota has been closely linked to the mucosal immune and been regarded as a reliable target for intestinal inflammation.
31759038	7	53	theme	intestinal	1122:1131	arg1	inflammation					1133:1144	the intestinal inflammation	1118:1144	the intestinal inflammation	1118:1144	KEY FINDINGS Both P-qua and P-tri relieved the intestinal inflammation and improved the functions of multiple barriers with increased integrity of mucous layer, enhanced transepithelial electrical resistance, declined epithelial and endothelial permeability to macromolecules in DSS-colitis.
31759038	10	54	theme	inflamed	1906:1913	arg1	gut					1915:1917	the inflamed gut	1902:1917	the inflamed gut	1902:1917	SIGNIFICANCE Aerobe-contained P-qua was a powerful adjuvant therapy for chronic colitis, via restoring the intestinal microflora and recovering the multi-barriers in the inflamed gut.
31759038	3	55	theme	Chronic	575:581	arg1	colitis					583:589	MATERIALS AND METHODS Chronic colitis	553:589	MATERIALS AND METHODS Chronic colitis	553:589	MATERIALS AND METHODS Chronic colitis was induced by dextran sulfate sodium (DSS) in C57BL/6 mice.
31759038	7	56	from	permeability	1320:1331	arg1	DSS-colitis					1354:1364	DSS-colitis	1354:1364	DSS-colitis	1354:1364	KEY FINDINGS Both P-qua and P-tri relieved the intestinal inflammation and improved the functions of multiple barriers with increased integrity of mucous layer, enhanced transepithelial electrical resistance, declined epithelial and endothelial permeability to macromolecules in DSS-colitis.
31759038	1	57	theme	Gut	121:123	arg1	target					214:219	a reliable target	203:219	a reliable target for intestinal inflammation	203:247	AIMS Gut microbiota has been closely linked to the mucosal immune and been regarded as a reliable target for intestinal inflammation.
31759038	1	57	theme	Gut	121:123	arg1	microbiota					125:134	AIMS Gut microbiota	116:134	AIMS Gut microbiota	116:134	AIMS Gut microbiota has been closely linked to the mucosal immune and been regarded as a reliable target for intestinal inflammation.
31759038	2	58	dep	aerobic	473:479	arg1	P-tri					466:470	P-tri	466:470	P-tri	466:470	This study aimed to explore the therapeutic roles of probiotic mixtures of Bifidobacterium infantis, Lactobacillus acidophilus, Enterococcus faecalis with (quadruple probiotics, P-qua) or without (triple probiotics, P-tri) aerobic Bacillus cereus in colitis, focusing on the multiple barrier functions.
31759038	2	58	dep	aerobic	473:479	arg1	probiotics					454:463	triple probiotics	447:463	triple probiotics	447:463	This study aimed to explore the therapeutic roles of probiotic mixtures of Bifidobacterium infantis, Lactobacillus acidophilus, Enterococcus faecalis with (quadruple probiotics, P-qua) or without (triple probiotics, P-tri) aerobic Bacillus cereus in colitis, focusing on the multiple barrier functions.
31759038	9	59	theme	increased	1652:1660	arg1	DSS-colitis					1628:1638	DSS-colitis	1628:1638	DSS-colitis	1628:1638	Indeed, P-qua was effective in rebuilding the structure and diversity of gut flora in DSS-colitis, especially increased abundance of Bifidobacterium, Akkermansia, Lactobacillus and Bacteroides.
31759038	9	59	theme	increased	1652:1660	arg1	abundance					1662:1670	increased abundance	1652:1670	increased abundance of Bifidobacterium, Akkermansia, Lactobacillus and Bacteroides	1652:1733	Indeed, P-qua was effective in rebuilding the structure and diversity of gut flora in DSS-colitis, especially increased abundance of Bifidobacterium, Akkermansia, Lactobacillus and Bacteroides.
31759038	0	60	with	mixtures	10:17	arg1	constituent					32:42	aerobic constituent	24:42	aerobic constituent	24:42	Probiotic mixtures with aerobic constituent promoted the recovery of multi-barriers in DSS-induced chronic colitis.
31759038	2	61	theme	infantis	341:348	arg1	mixtures					313:320	probiotic mixtures	303:320	probiotic mixtures of Bifidobacterium infantis, Lactobacillus acidophilus, Enterococcus faecalis with (quadruple probiotics, P-qua)	303:433	This study aimed to explore the therapeutic roles of probiotic mixtures of Bifidobacterium infantis, Lactobacillus acidophilus, Enterococcus faecalis with (quadruple probiotics, P-qua) or without (triple probiotics, P-tri) aerobic Bacillus cereus in colitis, focusing on the multiple barrier functions.
31759038	3	62	theme	sulfate	614:620	arg1	DSS					630:632	DSS	630:632	DSS	630:632	MATERIALS AND METHODS Chronic colitis was induced by dextran sulfate sodium (DSS) in C57BL/6 mice.
31759038	3	62	theme	sulfate	614:620	arg1	sodium					622:627	dextran sulfate sodium	606:627	dextran sulfate sodium (DSS) in C57BL/6 mice	606:649	MATERIALS AND METHODS Chronic colitis was induced by dextran sulfate sodium (DSS) in C57BL/6 mice.
31759038	9	63	theme	flora	1619:1623	arg1	diversity					1602:1610	diversity	1602:1610	diversity	1602:1610	Indeed, P-qua was effective in rebuilding the structure and diversity of gut flora in DSS-colitis, especially increased abundance of Bifidobacterium, Akkermansia, Lactobacillus and Bacteroides.
31759038	9	63	theme	flora	1619:1623	arg1	structure					1588:1596	structure	1588:1596	structure	1588:1596	Indeed, P-qua was effective in rebuilding the structure and diversity of gut flora in DSS-colitis, especially increased abundance of Bifidobacterium, Akkermansia, Lactobacillus and Bacteroides.
31759038	5	64	theme	epithelial	904:913	arg1	barrier					915:921	epithelial barrier	904:921	epithelial barrier	904:921	The intestinal inflammation and functions of multiple barriers were assessed, including the mucus barrier, epithelial barrier and endothelial barrier known as gut-vascular barrier (GVB).
31759038	3	65	theme	C57BL/6	638:644	arg1	mice					646:649	C57BL/6 mice	638:649	C57BL/6 mice	638:649	MATERIALS AND METHODS Chronic colitis was induced by dextran sulfate sodium (DSS) in C57BL/6 mice.
31759038	5	66	theme	intestinal	801:810	arg1	inflammation					812:823	intestinal inflammation	801:823	intestinal inflammation	801:823	The intestinal inflammation and functions of multiple barriers were assessed, including the mucus barrier, epithelial barrier and endothelial barrier known as gut-vascular barrier (GVB).
31759038	10	67	theme	powerful	1778:1785	arg1	therapy					1796:1802	a powerful adjuvant therapy	1776:1802	a powerful adjuvant therapy for chronic colitis	1776:1822	SIGNIFICANCE Aerobe-contained P-qua was a powerful adjuvant therapy for chronic colitis, via restoring the intestinal microflora and recovering the multi-barriers in the inflamed gut.
31759038	10	67	theme	powerful	1778:1785	arg1	P-qua					1766:1770	SIGNIFICANCE Aerobe-contained P-qua	1736:1770	SIGNIFICANCE Aerobe-contained P-qua	1736:1770	SIGNIFICANCE Aerobe-contained P-qua was a powerful adjuvant therapy for chronic colitis, via restoring the intestinal microflora and recovering the multi-barriers in the inflamed gut.
31759038	8	68	theme	active	1406:1411	arg1	role					1413:1416	a more active role	1399:1416	a more active role	1399:1416	Aerobe-contained P-qua revealed a more active role in barrier recovering relative to P-tri, while FMT as a positive control seemed to get better results than pure probiotics.
31759038	10	69	theme	SIGNIFICANCE	1736:1747	arg1	therapy					1796:1802	a powerful adjuvant therapy	1776:1802	a powerful adjuvant therapy for chronic colitis	1776:1822	SIGNIFICANCE Aerobe-contained P-qua was a powerful adjuvant therapy for chronic colitis, via restoring the intestinal microflora and recovering the multi-barriers in the inflamed gut.
31759038	10	69	theme	SIGNIFICANCE	1736:1747	arg1	P-qua					1766:1770	SIGNIFICANCE Aerobe-contained P-qua	1736:1770	SIGNIFICANCE Aerobe-contained P-qua	1736:1770	SIGNIFICANCE Aerobe-contained P-qua was a powerful adjuvant therapy for chronic colitis, via restoring the intestinal microflora and recovering the multi-barriers in the inflamed gut.
31759038	0	70	theme	multi-barriers	69:82	arg1	recovery					57:64	the recovery	53:64	the recovery of multi-barriers in DSS-induced chronic colitis	53:113	Probiotic mixtures with aerobic constituent promoted the recovery of multi-barriers in DSS-induced chronic colitis.
31759038	6	71	theme	gut	1021:1023	arg1	microbiota					1025:1034	gut microbiota	1021:1034	gut microbiota	1021:1034	Altered composition and diversity in gut microbiota were observed via sequencing analysis.
31759038	4	72	theme	positive	779:786	arg1	control					788:794	a positive control	777:794	a positive control	777:794	The probiotic mixtures P-qua or P-tri was gavage administrated respectively, while fecal microbiota transplantation (FMT) as a positive control.
31759038	7	73	theme	electrical	1261:1270	arg1	resistance					1272:1281	transepithelial electrical resistance	1245:1281	transepithelial electrical resistance	1245:1281	KEY FINDINGS Both P-qua and P-tri relieved the intestinal inflammation and improved the functions of multiple barriers with increased integrity of mucous layer, enhanced transepithelial electrical resistance, declined epithelial and endothelial permeability to macromolecules in DSS-colitis.
31759038	1	74	theme	intestinal	225:234	arg1	inflammation					236:247	intestinal inflammation	225:247	intestinal inflammation	225:247	AIMS Gut microbiota has been closely linked to the mucosal immune and been regarded as a reliable target for intestinal inflammation.
31759038	8	75	theme	pure	1525:1528	arg1	probiotics					1530:1539	pure probiotics	1525:1539	pure probiotics	1525:1539	Aerobe-contained P-qua revealed a more active role in barrier recovering relative to P-tri, while FMT as a positive control seemed to get better results than pure probiotics.
31759038	3	76	theme	dextran	606:612	arg1	DSS					630:632	DSS	630:632	DSS	630:632	MATERIALS AND METHODS Chronic colitis was induced by dextran sulfate sodium (DSS) in C57BL/6 mice.
31759038	3	76	theme	dextran	606:612	arg1	sodium					622:627	dextran sulfate sodium	606:627	dextran sulfate sodium (DSS) in C57BL/6 mice	606:649	MATERIALS AND METHODS Chronic colitis was induced by dextran sulfate sodium (DSS) in C57BL/6 mice.
31759038	4	77	theme	probiotic	656:664	arg1	P-qua					675:679	P-qua	675:679	P-qua	675:679	The probiotic mixtures P-qua or P-tri was gavage administrated respectively, while fecal microbiota transplantation (FMT) as a positive control.
31759038	4	77	theme	probiotic	656:664	arg1	gavage					694:699	gavage	694:699	gavage administrated respectively	694:726	The probiotic mixtures P-qua or P-tri was gavage administrated respectively, while fecal microbiota transplantation (FMT) as a positive control.
31759038	4	77	theme	probiotic	656:664	arg1	mixtures					666:673	The probiotic mixtures	652:673	The probiotic mixtures P-qua or P-tri	652:688	The probiotic mixtures P-qua or P-tri was gavage administrated respectively, while fecal microbiota transplantation (FMT) as a positive control.
31759038	4	77	theme	probiotic	656:664	arg1	P-tri					684:688	P-tri	684:688	P-tri	684:688	The probiotic mixtures P-qua or P-tri was gavage administrated respectively, while fecal microbiota transplantation (FMT) as a positive control.
30009912	7	0	theme	matrix	983:988	arg1	cavities					943:950	the cavities	939:950	the cavities of a continuous polysaccharide matrix	939:988	Indeed, the encapsulation of magadiite was confirmed by the SEM images, which is presented as micron free aggregates included in the cavities of a continuous polysaccharide matrix.
30009912	4	1	theme	copper	511:516	arg1	ions					518:521	loaded copper ions	504:521	loaded copper ions	504:521	These beads were then contacted with a solution of NaBH4 in order to reduce loaded copper ions into copper nanoparticles species.
30009912	5	2	theme	UV-visible	655:664	arg1	DR					666:667	UV-visible DR	655:667	UV-visible DR	655:667	The resulting nanocomposite (Cu-NPs-magadiite/chitosan) was characterized by XRD, FTIR, SEM, TG, UV-visible DR and EDX analysis.
30009912	7	3	theme	continuous	957:966	arg1	matrix					983:988	a continuous polysaccharide matrix	955:988	a continuous polysaccharide matrix	955:988	Indeed, the encapsulation of magadiite was confirmed by the SEM images, which is presented as micron free aggregates included in the cavities of a continuous polysaccharide matrix.
30009912	2	4	from	matrix	334:339	arg1	dispersion					287:296	direct dispersion	280:296	direct dispersion of the Cu-magadiite in the chitosan matrix	280:339	The synthesis was carried out by direct dispersion of the Cu-magadiite in the chitosan matrix.
30009912	2	5	theme	Cu-magadiite	305:316	arg1	dispersion					287:296	direct dispersion	280:296	direct dispersion of the Cu-magadiite in the chitosan matrix	280:339	The synthesis was carried out by direct dispersion of the Cu-magadiite in the chitosan matrix.
30009912	4	6	theme	NaBH4	479:483	arg1	solution					467:474	a solution	465:474	a solution of NaBH4	465:483	These beads were then contacted with a solution of NaBH4 in order to reduce loaded copper ions into copper nanoparticles species.
30009912	9	7	theme	disc	1198:1201	arg1	method					1214:1219	the disc inhibition method	1194:1219	the disc inhibition method	1194:1219	The antibacterial activity against E. coli and S. aureus was highlighted by the disc inhibition method and the minimum inhibitory concentration (MIC) was determined.
30009912	7	8	theme	SEM	870:872	arg1	images					874:879	the SEM images	866:879	the SEM images	866:879	Indeed, the encapsulation of magadiite was confirmed by the SEM images, which is presented as micron free aggregates included in the cavities of a continuous polysaccharide matrix.
30009912	7	8	theme	SEM	870:872	arg1	aggregates					916:925	micron free aggregates	904:925	micron free aggregates included in the cavities of a continuous polysaccharide matrix	904:988	Indeed, the encapsulation of magadiite was confirmed by the SEM images, which is presented as micron free aggregates included in the cavities of a continuous polysaccharide matrix.
30009912	1	9	theme	copper	190:195	arg1	Cu-magadiite					218:229	Cu-magadiite	218:229	Cu-magadiite	218:229	In this work, an inorganic-organic nanocomposite was prepared by combining copper exchanged-magadiite (Cu-magadiite) with chitosan.
30009912	1	9	theme	copper	190:195	arg1	exchanged-magadiite					197:215	copper exchanged-magadiite	190:215	copper exchanged-magadiite (Cu-magadiite)	190:230	In this work, an inorganic-organic nanocomposite was prepared by combining copper exchanged-magadiite (Cu-magadiite) with chitosan.
30009912	6	10	theme	composite	799:807	arg1	formation					764:772	the formation	760:772	the formation of the organic-inorganic composite	760:807	The results show that the magadiite was completely exfoliated confirming the formation of the organic-inorganic composite.
30009912	10	11	theme	pathogen	1384:1391	arg1	bacteria					1393:1400	both pathogen bacteria	1379:1400	both pathogen bacteria	1379:1400	The CuNPs-magadiite/chitosan nanocomposite showed a very efficient bactericidal effect against both pathogen bacteria.
30009912	0	12	theme	nanocomposite	25:37	arg1	beads					39:43	CuNPs-magadiite/chitosan nanocomposite beads	0:43	CuNPs-magadiite/chitosan nanocomposite beads as advanced antibacterial agent: Synthetic path and characterization.	0:113	CuNPs-magadiite/chitosan nanocomposite beads as advanced antibacterial agent: Synthetic path and characterization.
30009912	0	12	theme	nanocomposite	25:37	arg1	characterization					97:112	characterization	97:112	characterization	97:112	CuNPs-magadiite/chitosan nanocomposite beads as advanced antibacterial agent: Synthetic path and characterization.
30009912	0	12	theme	nanocomposite	25:37	arg1	path					88:91	Synthetic path	78:91	Synthetic path	78:91	CuNPs-magadiite/chitosan nanocomposite beads as advanced antibacterial agent: Synthetic path and characterization.
30009912	6	13	theme	organic-inorganic	781:797	arg1	composite					799:807	the organic-inorganic composite	777:807	the organic-inorganic composite	777:807	The results show that the magadiite was completely exfoliated confirming the formation of the organic-inorganic composite.
30009912	0	14	theme	CuNPs-magadiite/chitosan	0:23	arg1	beads					39:43	CuNPs-magadiite/chitosan nanocomposite beads	0:43	CuNPs-magadiite/chitosan nanocomposite beads as advanced antibacterial agent: Synthetic path and characterization.	0:113	CuNPs-magadiite/chitosan nanocomposite beads as advanced antibacterial agent: Synthetic path and characterization.
30009912	0	14	theme	CuNPs-magadiite/chitosan	0:23	arg1	characterization					97:112	characterization	97:112	characterization	97:112	CuNPs-magadiite/chitosan nanocomposite beads as advanced antibacterial agent: Synthetic path and characterization.
30009912	0	14	theme	CuNPs-magadiite/chitosan	0:23	arg1	path					88:91	Synthetic path	78:91	Synthetic path	78:91	CuNPs-magadiite/chitosan nanocomposite beads as advanced antibacterial agent: Synthetic path and characterization.
30009912	7	15	theme	magadiite	839:847	arg1	encapsulation					822:834	the encapsulation	818:834	the encapsulation of magadiite	818:847	Indeed, the encapsulation of magadiite was confirmed by the SEM images, which is presented as micron free aggregates included in the cavities of a continuous polysaccharide matrix.
30009912	2	16	theme	chitosan	325:332	arg1	matrix					334:339	the chitosan matrix	321:339	the chitosan matrix	321:339	The synthesis was carried out by direct dispersion of the Cu-magadiite in the chitosan matrix.
30009912	11	17	theme	0.50 μg/L	1501:1509	arg1	0.25 μg/L					1466:1474	0.25 μg/L	1466:1474	0.25 μg/L	1466:1474	Additionally, the MIC values obtained for nanocomposite are of 0.25 μg/L against S. aureus and of 0.50 μg/L against E. coli.
30009912	3	18	theme	average	392:398	arg1	diameter					400:407	an average diameter	389:407	an average diameter of about 1-1.2 mm	389:425	The mixture obtained is shaped into beads with an average diameter of about 1-1.2 mm.
30009912	9	19	theme	minimum	1229:1235	arg1	concentration					1248:1260	the minimum inhibitory concentration	1225:1260	the minimum inhibitory concentration (MIC)	1225:1266	The antibacterial activity against E. coli and S. aureus was highlighted by the disc inhibition method and the minimum inhibitory concentration (MIC) was determined.
30009912	9	19	theme	minimum	1229:1235	arg1	MIC					1263:1265	MIC	1263:1265	MIC	1263:1265	The antibacterial activity against E. coli and S. aureus was highlighted by the disc inhibition method and the minimum inhibitory concentration (MIC) was determined.
30009912	2	20	from	dispersion	287:296	arg1	matrix					334:339	the chitosan matrix	321:339	the chitosan matrix	321:339	The synthesis was carried out by direct dispersion of the Cu-magadiite in the chitosan matrix.
30009912	10	21	theme	efficient	1341:1349	arg1	effect					1364:1369	a very efficient bactericidal effect	1334:1369	a very efficient bactericidal effect against both pathogen bacteria	1334:1400	The CuNPs-magadiite/chitosan nanocomposite showed a very efficient bactericidal effect against both pathogen bacteria.
30009912	0	22	theme	antibacterial	57:69	arg1	agent					71:75	advanced antibacterial agent	48:75	advanced antibacterial agent	48:75	CuNPs-magadiite/chitosan nanocomposite beads as advanced antibacterial agent: Synthetic path and characterization.
30009912	10	23	theme	CuNPs-magadiite/chitosan	1288:1311	arg1	nanocomposite					1313:1325	The CuNPs-magadiite/chitosan nanocomposite	1284:1325	The CuNPs-magadiite/chitosan nanocomposite	1284:1325	The CuNPs-magadiite/chitosan nanocomposite showed a very efficient bactericidal effect against both pathogen bacteria.
30009912	8	24	theme	solid	1104:1108	arg1	matrix					1110:1115	the magadiite-chitosan solid matrix	1081:1115	the magadiite-chitosan solid matrix	1081:1115	Otherwise, they confirm also the formation of CuNPs which are probably immobilized inside the magadiite-chitosan solid matrix.
30009912	9	25	theme	inhibitory	1237:1246	arg1	concentration					1248:1260	the minimum inhibitory concentration	1225:1260	the minimum inhibitory concentration (MIC)	1225:1266	The antibacterial activity against E. coli and S. aureus was highlighted by the disc inhibition method and the minimum inhibitory concentration (MIC) was determined.
30009912	9	25	theme	inhibitory	1237:1246	arg1	MIC					1263:1265	MIC	1263:1265	MIC	1263:1265	The antibacterial activity against E. coli and S. aureus was highlighted by the disc inhibition method and the minimum inhibitory concentration (MIC) was determined.
30009912	9	26	theme	antibacterial	1122:1134	arg1	activity					1136:1143	The antibacterial activity	1118:1143	The antibacterial activity against E. coli and S. aureus	1118:1173	The antibacterial activity against E. coli and S. aureus was highlighted by the disc inhibition method and the minimum inhibitory concentration (MIC) was determined.
30009912	0	27	theme	advanced	48:55	arg1	agent					71:75	advanced antibacterial agent	48:75	advanced antibacterial agent	48:75	CuNPs-magadiite/chitosan nanocomposite beads as advanced antibacterial agent: Synthetic path and characterization.
30009912	4	28	theme	copper	528:533	arg1	species					549:555	copper nanoparticles species	528:555	copper nanoparticles species	528:555	These beads were then contacted with a solution of NaBH4 in order to reduce loaded copper ions into copper nanoparticles species.
30009912	8	29	theme	magadiite-chitosan	1085:1102	arg1	matrix					1110:1115	the magadiite-chitosan solid matrix	1081:1115	the magadiite-chitosan solid matrix	1081:1115	Otherwise, they confirm also the formation of CuNPs which are probably immobilized inside the magadiite-chitosan solid matrix.
30009912	3	30	with	beads	378:382	arg1	diameter					400:407	an average diameter	389:407	an average diameter of about 1-1.2 mm	389:425	The mixture obtained is shaped into beads with an average diameter of about 1-1.2 mm.
30009912	3	31	theme	1-1.2 mm	418:425	arg1	diameter					400:407	an average diameter	389:407	an average diameter of about 1-1.2 mm	389:425	The mixture obtained is shaped into beads with an average diameter of about 1-1.2 mm.
30009912	5	32	theme	EDX	673:675	arg1	analysis					677:684	EDX analysis	673:684	EDX analysis	673:684	The resulting nanocomposite (Cu-NPs-magadiite/chitosan) was characterized by XRD, FTIR, SEM, TG, UV-visible DR and EDX analysis.
30009912	5	33	theme	resulting	562:570	arg1	Cu-NPs-magadiite/chitosan					587:611	Cu-NPs-magadiite/chitosan	587:611	Cu-NPs-magadiite/chitosan	587:611	The resulting nanocomposite (Cu-NPs-magadiite/chitosan) was characterized by XRD, FTIR, SEM, TG, UV-visible DR and EDX analysis.
30009912	5	33	theme	resulting	562:570	arg1	nanocomposite					572:584	The resulting nanocomposite	558:584	The resulting nanocomposite (Cu-NPs-magadiite/chitosan)	558:612	The resulting nanocomposite (Cu-NPs-magadiite/chitosan) was characterized by XRD, FTIR, SEM, TG, UV-visible DR and EDX analysis.
30009912	8	34	theme	CuNPs	1037:1041	arg1	formation					1024:1032	the formation	1020:1032	the formation of CuNPs which are probably immobilized inside the magadiite-chitosan solid matrix	1020:1115	Otherwise, they confirm also the formation of CuNPs which are probably immobilized inside the magadiite-chitosan solid matrix.
30009912	7	35	theme	micron	904:909	arg1	aggregates					916:925	micron free aggregates	904:925	micron free aggregates included in the cavities of a continuous polysaccharide matrix	904:988	Indeed, the encapsulation of magadiite was confirmed by the SEM images, which is presented as micron free aggregates included in the cavities of a continuous polysaccharide matrix.
30009912	7	35	theme	micron	904:909	arg1	images					874:879	the SEM images	866:879	the SEM images	866:879	Indeed, the encapsulation of magadiite was confirmed by the SEM images, which is presented as micron free aggregates included in the cavities of a continuous polysaccharide matrix.
30009912	4	36	theme	nanoparticles	535:547	arg1	species					549:555	copper nanoparticles species	528:555	copper nanoparticles species	528:555	These beads were then contacted with a solution of NaBH4 in order to reduce loaded copper ions into copper nanoparticles species.
30009912	9	37	theme	inhibition	1203:1212	arg1	method					1214:1219	the disc inhibition method	1194:1219	the disc inhibition method	1194:1219	The antibacterial activity against E. coli and S. aureus was highlighted by the disc inhibition method and the minimum inhibitory concentration (MIC) was determined.
30009912	10	38	theme	bactericidal	1351:1362	arg1	effect					1364:1369	a very efficient bactericidal effect	1334:1369	a very efficient bactericidal effect against both pathogen bacteria	1334:1400	The CuNPs-magadiite/chitosan nanocomposite showed a very efficient bactericidal effect against both pathogen bacteria.
30009912	1	39	theme	inorganic-organic	132:148	arg1	nanocomposite					150:162	an inorganic-organic nanocomposite	129:162	an inorganic-organic nanocomposite	129:162	In this work, an inorganic-organic nanocomposite was prepared by combining copper exchanged-magadiite (Cu-magadiite) with chitosan.
30009912	7	40	theme	free	911:914	arg1	aggregates					916:925	micron free aggregates	904:925	micron free aggregates included in the cavities of a continuous polysaccharide matrix	904:988	Indeed, the encapsulation of magadiite was confirmed by the SEM images, which is presented as micron free aggregates included in the cavities of a continuous polysaccharide matrix.
30009912	7	40	theme	free	911:914	arg1	images					874:879	the SEM images	866:879	the SEM images	866:879	Indeed, the encapsulation of magadiite was confirmed by the SEM images, which is presented as micron free aggregates included in the cavities of a continuous polysaccharide matrix.
30009912	0	41	dep	beads	39:43	arg1	beads					39:43	CuNPs-magadiite/chitosan nanocomposite beads	0:43	CuNPs-magadiite/chitosan nanocomposite beads as advanced antibacterial agent: Synthetic path and characterization.	0:113	CuNPs-magadiite/chitosan nanocomposite beads as advanced antibacterial agent: Synthetic path and characterization.
30009912	0	41	dep	beads	39:43	arg1	characterization					97:112	characterization	97:112	characterization	97:112	CuNPs-magadiite/chitosan nanocomposite beads as advanced antibacterial agent: Synthetic path and characterization.
30009912	0	41	dep	beads	39:43	arg1	path					88:91	Synthetic path	78:91	Synthetic path	78:91	CuNPs-magadiite/chitosan nanocomposite beads as advanced antibacterial agent: Synthetic path and characterization.
30009912	11	42	theme	MIC	1421:1423	arg1	values					1425:1430	the MIC values	1417:1430	the MIC values obtained for nanocomposite	1417:1457	Additionally, the MIC values obtained for nanocomposite are of 0.25 μg/L against S. aureus and of 0.50 μg/L against E. coli.
30009912	2	43	from	Cu-magadiite	305:316	arg1	matrix					334:339	the chitosan matrix	321:339	the chitosan matrix	321:339	The synthesis was carried out by direct dispersion of the Cu-magadiite in the chitosan matrix.
30009912	0	44	theme	Synthetic	78:86	arg1	beads					39:43	CuNPs-magadiite/chitosan nanocomposite beads	0:43	CuNPs-magadiite/chitosan nanocomposite beads as advanced antibacterial agent: Synthetic path and characterization.	0:113	CuNPs-magadiite/chitosan nanocomposite beads as advanced antibacterial agent: Synthetic path and characterization.
30009912	0	44	theme	Synthetic	78:86	arg1	path					88:91	Synthetic path	78:91	Synthetic path	78:91	CuNPs-magadiite/chitosan nanocomposite beads as advanced antibacterial agent: Synthetic path and characterization.
30009912	4	45	theme	loaded	504:509	arg1	ions					518:521	loaded copper ions	504:521	loaded copper ions	504:521	These beads were then contacted with a solution of NaBH4 in order to reduce loaded copper ions into copper nanoparticles species.
30009912	7	46	theme	polysaccharide	968:981	arg1	matrix					983:988	a continuous polysaccharide matrix	955:988	a continuous polysaccharide matrix	955:988	Indeed, the encapsulation of magadiite was confirmed by the SEM images, which is presented as micron free aggregates included in the cavities of a continuous polysaccharide matrix.
30009912	2	47	theme	direct	280:285	arg1	dispersion					287:296	direct dispersion	280:296	direct dispersion of the Cu-magadiite in the chitosan matrix	280:339	The synthesis was carried out by direct dispersion of the Cu-magadiite in the chitosan matrix.
31812896	10	0	theme	illnesses	1985:1993	arg1	development					1963:1973	the development	1959:1973	the development of mental illnesses in adulthood	1959:2006	Therefore, colonizing the gut with beneficial microbes may protect against the development of mental illnesses in adulthood.
31812896	6	1	theme	pubertal	1213:1220	arg1	development					1222:1232	pubertal development	1213:1232	pubertal development	1213:1232	Given the beneficial effects of Lactobacillus strains on stress and behaviour, we next investigated whether replenishment of the gut with the probiotic Lactobacillus reuteri (L. reuteri) throughout pubertal development would modulate LPS-induced sickness and enduring effects on memory dysfunction, anxiety-like behaviour and stress reactivity in adulthood (Experiment 2).
31812896	10	2	theme	beneficial	1919:1928	arg1	microbes					1930:1937	beneficial microbes	1919:1937	beneficial microbes	1919:1937	Therefore, colonizing the gut with beneficial microbes may protect against the development of mental illnesses in adulthood.
31812896	1	3	theme	critical	143:150	arg1	Puberty					130:136	Puberty	130:136	Puberty	130:136	Puberty is a critical period of neural development, and exposure to stress and inflammation during this period is thought to increase vulnerability to mental illness.
31812896	1	3	theme	critical	143:150	arg1	period					152:157	a critical period	141:157	a critical period of neural development	141:179	Puberty is a critical period of neural development, and exposure to stress and inflammation during this period is thought to increase vulnerability to mental illness.
31812896	6	4	with	replenishment	1123:1135	arg1	reuteri					1181:1187	the probiotic Lactobacillus reuteri	1153:1187	the probiotic Lactobacillus reuteri (L. reuteri) throughout pubertal development	1153:1232	Given the beneficial effects of Lactobacillus strains on stress and behaviour, we next investigated whether replenishment of the gut with the probiotic Lactobacillus reuteri (L. reuteri) throughout pubertal development would modulate LPS-induced sickness and enduring effects on memory dysfunction, anxiety-like behaviour and stress reactivity in adulthood (Experiment 2).
31812896	0	5	theme	microbial	83:91	arg1	outcomes					93:100	the associated neurochemical and microbial outcomes	50:100	the associated neurochemical and microbial outcomes	50:100	Pubertal probiotic blocks LPS-induced anxiety and the associated neurochemical and microbial outcomes, in a sex dependent manner.
31812896	9	6	with	gut	1742:1744	arg1	reuteri					1754:1760	L. reuteri	1751:1760	L. reuteri	1751:1760	These findings demonstrate that colonizing the gut with L. reuteri during puberty modulates sickness responses and enduring behavioural and neurochemical outcomes in a sex-specific manner.
31812896	7	7	theme	enduring	1428:1435	arg1	changes					1437:1443	enduring changes	1428:1443	enduring changes in anxiety-like behaviors among males only	1428:1486	LPS treatment at 6 weeks of age created enduring changes in anxiety-like behaviors among males only.
31812896	5	8	theme	adult	806:810	arg1	counterparts					812:823	their adult counterparts	800:823	their adult counterparts	800:823	Compared to their adult counterparts, pubertal males and females showed more significant changes in gut microbial composition following LPS treatment, including the depletion of numerous bacterial genera such as Lactobacillus.
31812896	4	9	theme	age	549:551	arg1	differences					561:571	age and sex differences	549:571	differences	561:571	We first examined age and sex differences in gut microbial changes among CD-1 mice exposed to an immune challenge (lipopolysaccharide; LPS) at 6 weeks of age (during the pubertal stress-sensitive period) or at 10 weeks of age (in adulthood) (Experiment 1).
31812896	4	10	from	differences	561:571	arg1	changes					590:596	gut microbial changes	576:596	gut microbial changes	576:596	We first examined age and sex differences in gut microbial changes among CD-1 mice exposed to an immune challenge (lipopolysaccharide; LPS) at 6 weeks of age (during the pubertal stress-sensitive period) or at 10 weeks of age (in adulthood) (Experiment 1).
31812896	4	11	theme	pubertal	701:708	arg1	period					727:732	the pubertal stress-sensitive period	697:732	the pubertal stress-sensitive period	697:732	We first examined age and sex differences in gut microbial changes among CD-1 mice exposed to an immune challenge (lipopolysaccharide; LPS) at 6 weeks of age (during the pubertal stress-sensitive period) or at 10 weeks of age (in adulthood) (Experiment 1).
31812896	5	12	from	changes	877:883	arg1	composition					902:912	gut microbial composition	888:912	gut microbial composition following LPS treatment	888:936	Compared to their adult counterparts, pubertal males and females showed more significant changes in gut microbial composition following LPS treatment, including the depletion of numerous bacterial genera such as Lactobacillus.
31812896	4	13	theme	age	685:687	arg1	weeks					676:680	6 weeks	674:680	6 weeks of age (during the pubertal stress-sensitive period)	674:733	We first examined age and sex differences in gut microbial changes among CD-1 mice exposed to an immune challenge (lipopolysaccharide; LPS) at 6 weeks of age (during the pubertal stress-sensitive period) or at 10 weeks of age (in adulthood) (Experiment 1).
31812896	10	14	from	development	1963:1973	arg1	adulthood					1998:2006	adulthood	1998:2006	adulthood	1998:2006	Therefore, colonizing the gut with beneficial microbes may protect against the development of mental illnesses in adulthood.
31812896	8	15	theme	brain	1677:1681	arg1	activation					1683:1692	stress-induced brain activation	1662:1692	stress-induced brain activation	1662:1692	Similarly, only males showed the protective effects of L. reuteri supplementation during puberty in preventing longstanding LPS-induced changes in anxiety-like behavior and stress-induced brain activation.
31812896	4	16	theme	sex	557:559	arg1	differences					561:571	age and sex differences	549:571	differences	561:571	We first examined age and sex differences in gut microbial changes among CD-1 mice exposed to an immune challenge (lipopolysaccharide; LPS) at 6 weeks of age (during the pubertal stress-sensitive period) or at 10 weeks of age (in adulthood) (Experiment 1).
31812896	6	17	theme	memory	1294:1299	arg1	dysfunction					1301:1311	memory dysfunction	1294:1311	memory dysfunction	1294:1311	Given the beneficial effects of Lactobacillus strains on stress and behaviour, we next investigated whether replenishment of the gut with the probiotic Lactobacillus reuteri (L. reuteri) throughout pubertal development would modulate LPS-induced sickness and enduring effects on memory dysfunction, anxiety-like behaviour and stress reactivity in adulthood (Experiment 2).
31812896	6	18	theme	strains	1061:1067	arg1	effects					1036:1042	the beneficial effects	1021:1042	the beneficial effects of Lactobacillus strains on stress and behaviour	1021:1091	Given the beneficial effects of Lactobacillus strains on stress and behaviour, we next investigated whether replenishment of the gut with the probiotic Lactobacillus reuteri (L. reuteri) throughout pubertal development would modulate LPS-induced sickness and enduring effects on memory dysfunction, anxiety-like behaviour and stress reactivity in adulthood (Experiment 2).
31812896	4	19	dep	lipopolysaccharide	646:663	arg1	LPS					666:668	LPS	666:668	LPS	666:668	We first examined age and sex differences in gut microbial changes among CD-1 mice exposed to an immune challenge (lipopolysaccharide; LPS) at 6 weeks of age (during the pubertal stress-sensitive period) or at 10 weeks of age (in adulthood) (Experiment 1).
31812896	8	20	theme	stress-induced	1662:1675	arg1	activation					1683:1692	stress-induced brain activation	1662:1692	stress-induced brain activation	1662:1692	Similarly, only males showed the protective effects of L. reuteri supplementation during puberty in preventing longstanding LPS-induced changes in anxiety-like behavior and stress-induced brain activation.
31812896	3	21	theme	microbiome	410:419	arg1	role					394:397	the role	390:397	the role of the gut microbiome during puberty, a period during which mental health conditions tend to onset,	390:497	Yet, the role of the gut microbiome during puberty, a period during which mental health conditions tend to onset, remains largely uninvestigated.
31812896	7	22	theme	LPS	1388:1390	arg1	treatment					1392:1400	LPS treatment	1388:1400	LPS treatment at 6 weeks of age	1388:1418	LPS treatment at 6 weeks of age created enduring changes in anxiety-like behaviors among males only.
31812896	9	23	theme	enduring	1810:1817	arg1	outcomes					1849:1856	enduring behavioural and neurochemical outcomes	1810:1856	enduring behavioural and neurochemical outcomes	1810:1856	These findings demonstrate that colonizing the gut with L. reuteri during puberty modulates sickness responses and enduring behavioural and neurochemical outcomes in a sex-specific manner.
31812896	8	24	from	changes	1625:1631	arg1	activation					1683:1692	stress-induced brain activation	1662:1692	stress-induced brain activation	1662:1692	Similarly, only males showed the protective effects of L. reuteri supplementation during puberty in preventing longstanding LPS-induced changes in anxiety-like behavior and stress-induced brain activation.
31812896	8	24	from	changes	1625:1631	arg1	behavior					1649:1656	anxiety-like behavior	1636:1656	anxiety-like behavior	1636:1656	Similarly, only males showed the protective effects of L. reuteri supplementation during puberty in preventing longstanding LPS-induced changes in anxiety-like behavior and stress-induced brain activation.
31812896	4	25	theme	stress-sensitive	710:725	arg1	period					727:732	the pubertal stress-sensitive period	697:732	the pubertal stress-sensitive period	697:732	We first examined age and sex differences in gut microbial changes among CD-1 mice exposed to an immune challenge (lipopolysaccharide; LPS) at 6 weeks of age (during the pubertal stress-sensitive period) or at 10 weeks of age (in adulthood) (Experiment 1).
31812896	1	26	theme	neural	162:167	arg1	development					169:179	neural development	162:179	neural development	162:179	Puberty is a critical period of neural development, and exposure to stress and inflammation during this period is thought to increase vulnerability to mental illness.
31812896	6	27	theme	anxiety-like	1314:1325	arg1	behaviour					1327:1335	anxiety-like behaviour	1314:1335	anxiety-like behaviour	1314:1335	Given the beneficial effects of Lactobacillus strains on stress and behaviour, we next investigated whether replenishment of the gut with the probiotic Lactobacillus reuteri (L. reuteri) throughout pubertal development would modulate LPS-induced sickness and enduring effects on memory dysfunction, anxiety-like behaviour and stress reactivity in adulthood (Experiment 2).
31812896	6	28	dep	reuteri	1181:1187	arg1	reuteri					1193:1199	L. reuteri	1190:1199	L. reuteri	1190:1199	Given the beneficial effects of Lactobacillus strains on stress and behaviour, we next investigated whether replenishment of the gut with the probiotic Lactobacillus reuteri (L. reuteri) throughout pubertal development would modulate LPS-induced sickness and enduring effects on memory dysfunction, anxiety-like behaviour and stress reactivity in adulthood (Experiment 2).
31812896	2	29	theme	gut	301:303	arg1	microbiome					305:314	The gut microbiome	297:314	The gut microbiome	297:314	The gut microbiome influences brain functioning and behavior and impacts mental health.
31812896	8	30	theme	L.	1544:1545	arg1	supplementation					1555:1569	L. reuteri supplementation	1544:1569	L. reuteri supplementation	1544:1569	Similarly, only males showed the protective effects of L. reuteri supplementation during puberty in preventing longstanding LPS-induced changes in anxiety-like behavior and stress-induced brain activation.
31812896	1	31	theme	development	169:179	arg1	Puberty					130:136	Puberty	130:136	Puberty	130:136	Puberty is a critical period of neural development, and exposure to stress and inflammation during this period is thought to increase vulnerability to mental illness.
31812896	1	31	theme	development	169:179	arg1	period					152:157	a critical period	141:157	a critical period of neural development	141:179	Puberty is a critical period of neural development, and exposure to stress and inflammation during this period is thought to increase vulnerability to mental illness.
31812896	9	32	theme	sickness	1787:1794	arg1	responses					1796:1804	sickness responses	1787:1804	sickness responses	1787:1804	These findings demonstrate that colonizing the gut with L. reuteri during puberty modulates sickness responses and enduring behavioural and neurochemical outcomes in a sex-specific manner.
31812896	6	33	theme	enduring	1274:1281	arg1	effects					1283:1289	enduring effects	1274:1289	enduring effects on memory dysfunction, anxiety-like behaviour and stress reactivity	1274:1357	Given the beneficial effects of Lactobacillus strains on stress and behaviour, we next investigated whether replenishment of the gut with the probiotic Lactobacillus reuteri (L. reuteri) throughout pubertal development would modulate LPS-induced sickness and enduring effects on memory dysfunction, anxiety-like behaviour and stress reactivity in adulthood (Experiment 2).
31812896	3	34	theme	gut	406:408	arg1	microbiome					410:419	the gut microbiome	402:419	the gut microbiome	402:419	Yet, the role of the gut microbiome during puberty, a period during which mental health conditions tend to onset, remains largely uninvestigated.
31812896	0	35	theme	LPS-induced	26:36	arg1	anxiety					38:44	LPS-induced anxiety	26:44	LPS-induced anxiety	26:44	Pubertal probiotic blocks LPS-induced anxiety and the associated neurochemical and microbial outcomes, in a sex dependent manner.
31812896	6	36	from	sickness	1261:1268	arg1	dysfunction					1301:1311	memory dysfunction	1294:1311	memory dysfunction	1294:1311	Given the beneficial effects of Lactobacillus strains on stress and behaviour, we next investigated whether replenishment of the gut with the probiotic Lactobacillus reuteri (L. reuteri) throughout pubertal development would modulate LPS-induced sickness and enduring effects on memory dysfunction, anxiety-like behaviour and stress reactivity in adulthood (Experiment 2).
31812896	6	36	from	sickness	1261:1268	arg1	behaviour					1327:1335	anxiety-like behaviour	1314:1335	anxiety-like behaviour	1314:1335	Given the beneficial effects of Lactobacillus strains on stress and behaviour, we next investigated whether replenishment of the gut with the probiotic Lactobacillus reuteri (L. reuteri) throughout pubertal development would modulate LPS-induced sickness and enduring effects on memory dysfunction, anxiety-like behaviour and stress reactivity in adulthood (Experiment 2).
31812896	6	36	from	sickness	1261:1268	arg1	reactivity					1348:1357	stress reactivity	1341:1357	stress reactivity	1341:1357	Given the beneficial effects of Lactobacillus strains on stress and behaviour, we next investigated whether replenishment of the gut with the probiotic Lactobacillus reuteri (L. reuteri) throughout pubertal development would modulate LPS-induced sickness and enduring effects on memory dysfunction, anxiety-like behaviour and stress reactivity in adulthood (Experiment 2).
31812896	6	37	theme	gut	1144:1146	arg1	replenishment					1123:1135	replenishment	1123:1135	replenishment of the gut with the probiotic Lactobacillus reuteri (L. reuteri) throughout pubertal development	1123:1232	Given the beneficial effects of Lactobacillus strains on stress and behaviour, we next investigated whether replenishment of the gut with the probiotic Lactobacillus reuteri (L. reuteri) throughout pubertal development would modulate LPS-induced sickness and enduring effects on memory dysfunction, anxiety-like behaviour and stress reactivity in adulthood (Experiment 2).
31812896	4	38	theme	CD-1	604:607	arg1	mice					609:612	CD-1 mice	604:612	CD-1 mice exposed to an immune challenge (lipopolysaccharide; LPS) at 6 weeks of age (during the pubertal stress-sensitive period) or at 10 weeks of age (in adulthood) (Experiment 1)	604:785	We first examined age and sex differences in gut microbial changes among CD-1 mice exposed to an immune challenge (lipopolysaccharide; LPS) at 6 weeks of age (during the pubertal stress-sensitive period) or at 10 weeks of age (in adulthood) (Experiment 1).
31812896	6	39	from	effects	1283:1289	arg1	dysfunction					1301:1311	memory dysfunction	1294:1311	memory dysfunction	1294:1311	Given the beneficial effects of Lactobacillus strains on stress and behaviour, we next investigated whether replenishment of the gut with the probiotic Lactobacillus reuteri (L. reuteri) throughout pubertal development would modulate LPS-induced sickness and enduring effects on memory dysfunction, anxiety-like behaviour and stress reactivity in adulthood (Experiment 2).
31812896	6	39	from	effects	1283:1289	arg1	behaviour					1327:1335	anxiety-like behaviour	1314:1335	anxiety-like behaviour	1314:1335	Given the beneficial effects of Lactobacillus strains on stress and behaviour, we next investigated whether replenishment of the gut with the probiotic Lactobacillus reuteri (L. reuteri) throughout pubertal development would modulate LPS-induced sickness and enduring effects on memory dysfunction, anxiety-like behaviour and stress reactivity in adulthood (Experiment 2).
31812896	6	39	from	effects	1283:1289	arg1	reactivity					1348:1357	stress reactivity	1341:1357	stress reactivity	1341:1357	Given the beneficial effects of Lactobacillus strains on stress and behaviour, we next investigated whether replenishment of the gut with the probiotic Lactobacillus reuteri (L. reuteri) throughout pubertal development would modulate LPS-induced sickness and enduring effects on memory dysfunction, anxiety-like behaviour and stress reactivity in adulthood (Experiment 2).
31812896	0	40	theme	dependent	112:120	arg1	manner					122:127	a sex dependent manner	106:127	a sex dependent manner	106:127	Pubertal probiotic blocks LPS-induced anxiety and the associated neurochemical and microbial outcomes, in a sex dependent manner.
31812896	5	41	theme	genera	985:990	arg1	depletion					953:961	the depletion	949:961	the depletion of numerous bacterial genera such as Lactobacillus	949:1012	Compared to their adult counterparts, pubertal males and females showed more significant changes in gut microbial composition following LPS treatment, including the depletion of numerous bacterial genera such as Lactobacillus.
31812896	7	42	from	weeks	1407:1411	arg1	treatment					1392:1400	LPS treatment	1388:1400	LPS treatment at 6 weeks of age	1388:1418	LPS treatment at 6 weeks of age created enduring changes in anxiety-like behaviors among males only.
31812896	1	43	theme	mental	281:286	arg1	illness					288:294	mental illness	281:294	mental illness	281:294	Puberty is a critical period of neural development, and exposure to stress and inflammation during this period is thought to increase vulnerability to mental illness.
31812896	6	44	theme	Lactobacillus	1047:1059	arg1	strains					1061:1067	Lactobacillus strains	1047:1067	Lactobacillus strains	1047:1067	Given the beneficial effects of Lactobacillus strains on stress and behaviour, we next investigated whether replenishment of the gut with the probiotic Lactobacillus reuteri (L. reuteri) throughout pubertal development would modulate LPS-induced sickness and enduring effects on memory dysfunction, anxiety-like behaviour and stress reactivity in adulthood (Experiment 2).
31812896	2	45	theme	mental	370:375	arg1	health					377:382	mental health	370:382	mental health	370:382	The gut microbiome influences brain functioning and behavior and impacts mental health.
31812896	9	46	theme	behavioural	1819:1829	arg1	outcomes					1849:1856	enduring behavioural and neurochemical outcomes	1810:1856	enduring behavioural and neurochemical outcomes	1810:1856	These findings demonstrate that colonizing the gut with L. reuteri during puberty modulates sickness responses and enduring behavioural and neurochemical outcomes in a sex-specific manner.
31812896	8	47	theme	supplementation	1555:1569	arg1	effects					1533:1539	the protective effects	1518:1539	the protective effects of L. reuteri supplementation	1518:1569	Similarly, only males showed the protective effects of L. reuteri supplementation during puberty in preventing longstanding LPS-induced changes in anxiety-like behavior and stress-induced brain activation.
31812896	9	48	theme	sex-specific	1863:1874	arg1	manner					1876:1881	a sex-specific manner	1861:1881	a sex-specific manner	1861:1881	These findings demonstrate that colonizing the gut with L. reuteri during puberty modulates sickness responses and enduring behavioural and neurochemical outcomes in a sex-specific manner.
31812896	0	49	theme	associated	54:63	arg1	outcomes					93:100	the associated neurochemical and microbial outcomes	50:100	the associated neurochemical and microbial outcomes	50:100	Pubertal probiotic blocks LPS-induced anxiety and the associated neurochemical and microbial outcomes, in a sex dependent manner.
31812896	6	50	theme	LPS-induced	1249:1259	arg1	sickness					1261:1268	LPS-induced sickness	1249:1268	LPS-induced sickness	1249:1268	Given the beneficial effects of Lactobacillus strains on stress and behaviour, we next investigated whether replenishment of the gut with the probiotic Lactobacillus reuteri (L. reuteri) throughout pubertal development would modulate LPS-induced sickness and enduring effects on memory dysfunction, anxiety-like behaviour and stress reactivity in adulthood (Experiment 2).
31812896	7	51	from	changes	1437:1443	arg1	behaviors					1461:1469	anxiety-like behaviors	1448:1469	anxiety-like behaviors	1448:1469	LPS treatment at 6 weeks of age created enduring changes in anxiety-like behaviors among males only.
31812896	8	52	dep	L.	1544:1545	arg1	reuteri					1547:1553	reuteri	1547:1553	reuteri	1547:1553	Similarly, only males showed the protective effects of L. reuteri supplementation during puberty in preventing longstanding LPS-induced changes in anxiety-like behavior and stress-induced brain activation.
31812896	6	53	from	effects	1036:1042	arg1	behaviour					1083:1091	behaviour	1083:1091	behaviour	1083:1091	Given the beneficial effects of Lactobacillus strains on stress and behaviour, we next investigated whether replenishment of the gut with the probiotic Lactobacillus reuteri (L. reuteri) throughout pubertal development would modulate LPS-induced sickness and enduring effects on memory dysfunction, anxiety-like behaviour and stress reactivity in adulthood (Experiment 2).
31812896	6	53	from	effects	1036:1042	arg1	stress					1072:1077	stress	1072:1077	stress	1072:1077	Given the beneficial effects of Lactobacillus strains on stress and behaviour, we next investigated whether replenishment of the gut with the probiotic Lactobacillus reuteri (L. reuteri) throughout pubertal development would modulate LPS-induced sickness and enduring effects on memory dysfunction, anxiety-like behaviour and stress reactivity in adulthood (Experiment 2).
31812896	3	54	theme	mental	459:464	arg1	conditions					473:482	mental health conditions	459:482	mental health conditions	459:482	Yet, the role of the gut microbiome during puberty, a period during which mental health conditions tend to onset, remains largely uninvestigated.
31812896	9	55	theme	neurochemical	1835:1847	arg1	outcomes					1849:1856	enduring behavioural and neurochemical outcomes	1810:1856	enduring behavioural and neurochemical outcomes	1810:1856	These findings demonstrate that colonizing the gut with L. reuteri during puberty modulates sickness responses and enduring behavioural and neurochemical outcomes in a sex-specific manner.
31812896	8	56	theme	protective	1522:1531	arg1	effects					1533:1539	the protective effects	1518:1539	the protective effects of L. reuteri supplementation	1518:1569	Similarly, only males showed the protective effects of L. reuteri supplementation during puberty in preventing longstanding LPS-induced changes in anxiety-like behavior and stress-induced brain activation.
31812896	10	57	with	gut	1910:1912	arg1	microbes					1930:1937	beneficial microbes	1919:1937	beneficial microbes	1919:1937	Therefore, colonizing the gut with beneficial microbes may protect against the development of mental illnesses in adulthood.
31812896	4	58	theme	age	753:755	arg1	Experiment					773:782	Experiment 1	773:784	Experiment 1	773:784	We first examined age and sex differences in gut microbial changes among CD-1 mice exposed to an immune challenge (lipopolysaccharide; LPS) at 6 weeks of age (during the pubertal stress-sensitive period) or at 10 weeks of age (in adulthood) (Experiment 1).
31812896	4	58	theme	age	753:755	arg1	weeks					744:748	10 weeks	741:748	10 weeks of age (in adulthood) (Experiment 1)	741:785	We first examined age and sex differences in gut microbial changes among CD-1 mice exposed to an immune challenge (lipopolysaccharide; LPS) at 6 weeks of age (during the pubertal stress-sensitive period) or at 10 weeks of age (in adulthood) (Experiment 1).
31812896	3	59	theme	health	466:471	arg1	conditions					473:482	mental health conditions	459:482	mental health conditions	459:482	Yet, the role of the gut microbiome during puberty, a period during which mental health conditions tend to onset, remains largely uninvestigated.
31812896	8	60	theme	longstanding	1600:1611	arg1	changes					1625:1631	longstanding LPS-induced changes	1600:1631	longstanding LPS-induced changes in anxiety-like behavior and stress-induced brain activation	1600:1692	Similarly, only males showed the protective effects of L. reuteri supplementation during puberty in preventing longstanding LPS-induced changes in anxiety-like behavior and stress-induced brain activation.
31812896	10	61	theme	mental	1978:1983	arg1	illnesses					1985:1993	mental illnesses	1978:1993	mental illnesses	1978:1993	Therefore, colonizing the gut with beneficial microbes may protect against the development of mental illnesses in adulthood.
31812896	4	62	theme	immune	628:633	arg1	lipopolysaccharide					646:663	lipopolysaccharide	646:663	lipopolysaccharide	646:663	We first examined age and sex differences in gut microbial changes among CD-1 mice exposed to an immune challenge (lipopolysaccharide; LPS) at 6 weeks of age (during the pubertal stress-sensitive period) or at 10 weeks of age (in adulthood) (Experiment 1).
31812896	4	62	theme	immune	628:633	arg1	challenge					635:643	an immune challenge	625:643	an immune challenge (lipopolysaccharide; LPS)	625:669	We first examined age and sex differences in gut microbial changes among CD-1 mice exposed to an immune challenge (lipopolysaccharide; LPS) at 6 weeks of age (during the pubertal stress-sensitive period) or at 10 weeks of age (in adulthood) (Experiment 1).
31812896	5	63	theme	microbial	892:900	arg1	composition					902:912	gut microbial composition	888:912	gut microbial composition following LPS treatment	888:936	Compared to their adult counterparts, pubertal males and females showed more significant changes in gut microbial composition following LPS treatment, including the depletion of numerous bacterial genera such as Lactobacillus.
31812896	4	64	from	weeks	744:748	arg1	adulthood					761:769	adulthood	761:769	adulthood	761:769	We first examined age and sex differences in gut microbial changes among CD-1 mice exposed to an immune challenge (lipopolysaccharide; LPS) at 6 weeks of age (during the pubertal stress-sensitive period) or at 10 weeks of age (in adulthood) (Experiment 1).
31812896	5	65	theme	pubertal	826:833	arg1	males					835:839	pubertal males	826:839	pubertal males	826:839	Compared to their adult counterparts, pubertal males and females showed more significant changes in gut microbial composition following LPS treatment, including the depletion of numerous bacterial genera such as Lactobacillus.
31812896	4	66	theme	gut	576:578	arg1	changes					590:596	gut microbial changes	576:596	gut microbial changes	576:596	We first examined age and sex differences in gut microbial changes among CD-1 mice exposed to an immune challenge (lipopolysaccharide; LPS) at 6 weeks of age (during the pubertal stress-sensitive period) or at 10 weeks of age (in adulthood) (Experiment 1).
31812896	5	67	theme	gut	888:890	arg1	composition					902:912	gut microbial composition	888:912	gut microbial composition following LPS treatment	888:936	Compared to their adult counterparts, pubertal males and females showed more significant changes in gut microbial composition following LPS treatment, including the depletion of numerous bacterial genera such as Lactobacillus.
31812896	5	68	theme	numerous	966:973	arg1	genera					985:990	numerous bacterial genera	966:990	numerous bacterial genera such as Lactobacillus	966:1012	Compared to their adult counterparts, pubertal males and females showed more significant changes in gut microbial composition following LPS treatment, including the depletion of numerous bacterial genera such as Lactobacillus.
31812896	5	69	theme	significant	865:875	arg1	changes					877:883	more significant changes	860:883	more significant changes	860:883	Compared to their adult counterparts, pubertal males and females showed more significant changes in gut microbial composition following LPS treatment, including the depletion of numerous bacterial genera such as Lactobacillus.
31812896	5	69	theme	significant	865:875	arg1	depletion					953:961	the depletion	949:961	the depletion of numerous bacterial genera such as Lactobacillus	949:1012	Compared to their adult counterparts, pubertal males and females showed more significant changes in gut microbial composition following LPS treatment, including the depletion of numerous bacterial genera such as Lactobacillus.
31812896	6	70	theme	stress	1341:1346	arg1	reactivity					1348:1357	stress reactivity	1341:1357	stress reactivity	1341:1357	Given the beneficial effects of Lactobacillus strains on stress and behaviour, we next investigated whether replenishment of the gut with the probiotic Lactobacillus reuteri (L. reuteri) throughout pubertal development would modulate LPS-induced sickness and enduring effects on memory dysfunction, anxiety-like behaviour and stress reactivity in adulthood (Experiment 2).
31812896	5	71	theme	bacterial	975:983	arg1	genera					985:990	numerous bacterial genera	966:990	numerous bacterial genera such as Lactobacillus	966:1012	Compared to their adult counterparts, pubertal males and females showed more significant changes in gut microbial composition following LPS treatment, including the depletion of numerous bacterial genera such as Lactobacillus.
31812896	6	72	theme	beneficial	1025:1034	arg1	effects					1036:1042	the beneficial effects	1021:1042	the beneficial effects of Lactobacillus strains on stress and behaviour	1021:1091	Given the beneficial effects of Lactobacillus strains on stress and behaviour, we next investigated whether replenishment of the gut with the probiotic Lactobacillus reuteri (L. reuteri) throughout pubertal development would modulate LPS-induced sickness and enduring effects on memory dysfunction, anxiety-like behaviour and stress reactivity in adulthood (Experiment 2).
31812896	8	73	theme	LPS-induced	1613:1623	arg1	changes					1625:1631	longstanding LPS-induced changes	1600:1631	longstanding LPS-induced changes in anxiety-like behavior and stress-induced brain activation	1600:1692	Similarly, only males showed the protective effects of L. reuteri supplementation during puberty in preventing longstanding LPS-induced changes in anxiety-like behavior and stress-induced brain activation.
31812896	0	74	theme	neurochemical	65:77	arg1	outcomes					93:100	the associated neurochemical and microbial outcomes	50:100	the associated neurochemical and microbial outcomes	50:100	Pubertal probiotic blocks LPS-induced anxiety and the associated neurochemical and microbial outcomes, in a sex dependent manner.
31812896	7	75	theme	age	1416:1418	arg1	weeks					1407:1411	6 weeks	1405:1411	6 weeks of age	1405:1418	LPS treatment at 6 weeks of age created enduring changes in anxiety-like behaviors among males only.
31812896	5	76	theme	LPS	924:926	arg1	treatment					928:936	LPS treatment	924:936	LPS treatment	924:936	Compared to their adult counterparts, pubertal males and females showed more significant changes in gut microbial composition following LPS treatment, including the depletion of numerous bacterial genera such as Lactobacillus.
31812896	7	77	theme	anxiety-like	1448:1459	arg1	behaviors					1461:1469	anxiety-like behaviors	1448:1469	anxiety-like behaviors	1448:1469	LPS treatment at 6 weeks of age created enduring changes in anxiety-like behaviors among males only.
31812896	8	78	theme	anxiety-like	1636:1647	arg1	behavior					1649:1656	anxiety-like behavior	1636:1656	anxiety-like behavior	1636:1656	Similarly, only males showed the protective effects of L. reuteri supplementation during puberty in preventing longstanding LPS-induced changes in anxiety-like behavior and stress-induced brain activation.
31812896	4	79	theme	microbial	580:588	arg1	changes					590:596	gut microbial changes	576:596	gut microbial changes	576:596	We first examined age and sex differences in gut microbial changes among CD-1 mice exposed to an immune challenge (lipopolysaccharide; LPS) at 6 weeks of age (during the pubertal stress-sensitive period) or at 10 weeks of age (in adulthood) (Experiment 1).
31888844	7	0	theme	sourced	1090:1096	arg1	modifier					1134:1141	a potential gel modifier	1118:1141	a potential gel modifier in food industry	1118:1158	Therefore, naturally sourced CNC is suggested as a potential gel modifier in food industry.
31888844	7	0	theme	sourced	1090:1096	arg1	CNC					1098:1100	naturally sourced CNC	1080:1100	naturally sourced CNC	1080:1100	Therefore, naturally sourced CNC is suggested as a potential gel modifier in food industry.
31888844	2	1	theme	water	345:349	arg1	capacity					359:366	the water holding capacity	341:366	the water holding capacity	341:366	The results showed that the water holding capacity, gel strength, viscoelasticity, and thermal stability of the composite gels were improved by increasing the CNC concentration from 0 to 1.0 % (w/v).
31888844	6	2	theme	filling	973:979	arg1	effects					981:987	the active filling effects	962:987	the active filling effects of CNC	962:994	Moreover, the active filling effects of CNC contributed to the formation of a compact and homogeneous gel structure.
31888844	0	3	theme	Gelling	75:81	arg1	properties					83:92	Gelling properties	75:92	Gelling properties	75:92	Heat-induced whey protein isolate gels improved by cellulose nanocrystals: Gelling properties and microstructure.
31888844	5	4	theme	protein	865:871	arg1	matrixes					873:880	the protein matrixes	861:880	the protein matrixes	861:880	At higher CNC concentrations, the agglomerated CNC can serve as an active dehydrating agent to absorb moisture in the protein matrixes, which promotes unfolding and cross-linking of the protein molecules.
31888844	6	5	theme	gel	1054:1056	arg1	structure					1058:1066	a compact and homogeneous gel structure	1028:1066	a compact and homogeneous gel structure	1028:1066	Moreover, the active filling effects of CNC contributed to the formation of a compact and homogeneous gel structure.
31888844	1	6	theme	heat-induced	253:264	arg1	gels					293:296	heat-induced whey protein isolate (WPI) gels	253:296	heat-induced whey protein isolate (WPI) gels	253:296	Cellulose nanocrystals (CNC) were successfully prepared from wheat bran, and their effects on the gelling properties and microstructure of heat-induced whey protein isolate (WPI) gels were investigated.
31888844	6	7	theme	structure	1058:1066	arg1	formation					1015:1023	the formation	1011:1023	the formation of a compact and homogeneous gel structure	1011:1066	Moreover, the active filling effects of CNC contributed to the formation of a compact and homogeneous gel structure.
31888844	4	8	with	compatibility	673:685	arg1	matrixes					704:711	the protein matrixes	692:711	the protein matrixes	692:711	CNC has good compatibility with the protein matrixes at relatively low concentrations.
31888844	5	9	theme	protein	933:939	arg1	molecules					941:949	the protein molecules	929:949	the protein molecules	929:949	At higher CNC concentrations, the agglomerated CNC can serve as an active dehydrating agent to absorb moisture in the protein matrixes, which promotes unfolding and cross-linking of the protein molecules.
31888844	1	10	theme	Cellulose	114:122	arg1	CNC					138:140	CNC	138:140	CNC	138:140	Cellulose nanocrystals (CNC) were successfully prepared from wheat bran, and their effects on the gelling properties and microstructure of heat-induced whey protein isolate (WPI) gels were investigated.
31888844	1	10	theme	Cellulose	114:122	arg1	nanocrystals					124:135	Cellulose nanocrystals	114:135	Cellulose nanocrystals (CNC)	114:141	Cellulose nanocrystals (CNC) were successfully prepared from wheat bran, and their effects on the gelling properties and microstructure of heat-induced whey protein isolate (WPI) gels were investigated.
31888844	1	11	theme	whey	266:269	arg1	gels					293:296	heat-induced whey protein isolate (WPI) gels	253:296	heat-induced whey protein isolate (WPI) gels	253:296	Cellulose nanocrystals (CNC) were successfully prepared from wheat bran, and their effects on the gelling properties and microstructure of heat-induced whey protein isolate (WPI) gels were investigated.
31888844	5	12	theme	molecules	941:949	arg1	cross-linking					912:924	cross-linking	912:924	cross-linking	912:924	At higher CNC concentrations, the agglomerated CNC can serve as an active dehydrating agent to absorb moisture in the protein matrixes, which promotes unfolding and cross-linking of the protein molecules.
31888844	5	12	theme	molecules	941:949	arg1	unfolding					898:906	unfolding	898:906	unfolding	898:906	At higher CNC concentrations, the agglomerated CNC can serve as an active dehydrating agent to absorb moisture in the protein matrixes, which promotes unfolding and cross-linking of the protein molecules.
31888844	4	13	theme	good	668:671	arg1	compatibility					673:685	good compatibility	668:685	good compatibility with the protein matrixes	668:711	CNC has good compatibility with the protein matrixes at relatively low concentrations.
31888844	1	14	theme	protein	271:277	arg1	gels					293:296	heat-induced whey protein isolate (WPI) gels	253:296	heat-induced whey protein isolate (WPI) gels	253:296	Cellulose nanocrystals (CNC) were successfully prepared from wheat bran, and their effects on the gelling properties and microstructure of heat-induced whey protein isolate (WPI) gels were investigated.
31888844	7	15	theme	food	1146:1149	arg1	industry					1151:1158	food industry	1146:1158	food industry	1146:1158	Therefore, naturally sourced CNC is suggested as a potential gel modifier in food industry.
31888844	5	16	theme	agglomerated	781:792	arg1	CNC					794:796	the agglomerated CNC	777:796	the agglomerated CNC	777:796	At higher CNC concentrations, the agglomerated CNC can serve as an active dehydrating agent to absorb moisture in the protein matrixes, which promotes unfolding and cross-linking of the protein molecules.
31888844	5	16	theme	agglomerated	781:792	arg1	agent					833:837	an active dehydrating agent	811:837	an active dehydrating agent to absorb moisture in the protein matrixes, which promotes unfolding and cross-linking of the protein molecules	811:949	At higher CNC concentrations, the agglomerated CNC can serve as an active dehydrating agent to absorb moisture in the protein matrixes, which promotes unfolding and cross-linking of the protein molecules.
31888844	6	17	theme	compact	1030:1036	arg1	structure					1058:1066	a compact and homogeneous gel structure	1028:1066	a compact and homogeneous gel structure	1028:1066	Moreover, the active filling effects of CNC contributed to the formation of a compact and homogeneous gel structure.
31888844	1	18	theme	wheat	175:179	arg1	bran					181:184	wheat bran	175:184	wheat bran	175:184	Cellulose nanocrystals (CNC) were successfully prepared from wheat bran, and their effects on the gelling properties and microstructure of heat-induced whey protein isolate (WPI) gels were investigated.
31888844	7	19	from	modifier	1134:1141	arg1	industry					1151:1158	food industry	1146:1158	food industry	1146:1158	Therefore, naturally sourced CNC is suggested as a potential gel modifier in food industry.
31888844	2	20	theme	composite	429:437	arg1	gels					439:442	the composite gels	425:442	the composite gels	425:442	The results showed that the water holding capacity, gel strength, viscoelasticity, and thermal stability of the composite gels were improved by increasing the CNC concentration from 0 to 1.0 % (w/v).
31888844	5	21	theme	higher	750:755	arg1	concentrations					761:774	higher CNC concentrations	750:774	higher CNC concentrations	750:774	At higher CNC concentrations, the agglomerated CNC can serve as an active dehydrating agent to absorb moisture in the protein matrixes, which promotes unfolding and cross-linking of the protein molecules.
31888844	3	22	from	conversion	597:606	arg1	α-helix					640:646	α-helix	640:646	α-helix	640:646	The incorporation of CNC restricted water mobility and facilitated conformation conversion of the secondary structure from α-helix to β-sheet.
31888844	2	23	theme	gel	369:371	arg1	strength					373:380	gel strength	369:380	gel strength	369:380	The results showed that the water holding capacity, gel strength, viscoelasticity, and thermal stability of the composite gels were improved by increasing the CNC concentration from 0 to 1.0 % (w/v).
31888844	0	24	theme	Heat-induced	0:11	arg1	protein					18:24	Heat-induced whey protein	0:24	Heat-induced whey protein	0:24	Heat-induced whey protein isolate gels improved by cellulose nanocrystals: Gelling properties and microstructure.
31888844	1	25	theme	isolate	279:285	arg1	gels					293:296	heat-induced whey protein isolate (WPI) gels	253:296	heat-induced whey protein isolate (WPI) gels	253:296	Cellulose nanocrystals (CNC) were successfully prepared from wheat bran, and their effects on the gelling properties and microstructure of heat-induced whey protein isolate (WPI) gels were investigated.
31888844	7	26	theme	potential	1120:1128	arg1	modifier					1134:1141	a potential gel modifier	1118:1141	a potential gel modifier in food industry	1118:1158	Therefore, naturally sourced CNC is suggested as a potential gel modifier in food industry.
31888844	7	26	theme	potential	1120:1128	arg1	CNC					1098:1100	naturally sourced CNC	1080:1100	naturally sourced CNC	1080:1100	Therefore, naturally sourced CNC is suggested as a potential gel modifier in food industry.
31888844	4	27	theme	low	727:729	arg1	concentrations					731:744	relatively low concentrations	716:744	relatively low concentrations	716:744	CNC has good compatibility with the protein matrixes at relatively low concentrations.
31888844	2	28	theme	thermal	404:410	arg1	stability					412:420	thermal stability	404:420	thermal stability	404:420	The results showed that the water holding capacity, gel strength, viscoelasticity, and thermal stability of the composite gels were improved by increasing the CNC concentration from 0 to 1.0 % (w/v).
31888844	7	29	theme	gel	1130:1132	arg1	modifier					1134:1141	a potential gel modifier	1118:1141	a potential gel modifier in food industry	1118:1158	Therefore, naturally sourced CNC is suggested as a potential gel modifier in food industry.
31888844	7	29	theme	gel	1130:1132	arg1	CNC					1098:1100	naturally sourced CNC	1080:1100	naturally sourced CNC	1080:1100	Therefore, naturally sourced CNC is suggested as a potential gel modifier in food industry.
31888844	1	30	theme	WPI	288:290	arg1	gels					293:296	heat-induced whey protein isolate (WPI) gels	253:296	heat-induced whey protein isolate (WPI) gels	253:296	Cellulose nanocrystals (CNC) were successfully prepared from wheat bran, and their effects on the gelling properties and microstructure of heat-induced whey protein isolate (WPI) gels were investigated.
31888844	3	31	theme	secondary	615:623	arg1	structure					625:633	the secondary structure	611:633	the secondary structure from α-helix	611:646	The incorporation of CNC restricted water mobility and facilitated conformation conversion of the secondary structure from α-helix to β-sheet.
31888844	0	32	dep	nanocrystals	61:72	arg1	microstructure					98:111	microstructure	98:111	microstructure	98:111	Heat-induced whey protein isolate gels improved by cellulose nanocrystals: Gelling properties and microstructure.
31888844	0	32	dep	nanocrystals	61:72	arg1	properties					83:92	Gelling properties	75:92	Gelling properties	75:92	Heat-induced whey protein isolate gels improved by cellulose nanocrystals: Gelling properties and microstructure.
31888844	5	33	theme	CNC	757:759	arg1	concentrations					761:774	higher CNC concentrations	750:774	higher CNC concentrations	750:774	At higher CNC concentrations, the agglomerated CNC can serve as an active dehydrating agent to absorb moisture in the protein matrixes, which promotes unfolding and cross-linking of the protein molecules.
31888844	2	34	dep	1.0	504:506	arg1	to					501:502	to	501:502	to	501:502	The results showed that the water holding capacity, gel strength, viscoelasticity, and thermal stability of the composite gels were improved by increasing the CNC concentration from 0 to 1.0 % (w/v).
31888844	3	35	theme	structure	625:633	arg1	conversion					597:606	conformation conversion	584:606	conformation conversion of the secondary structure from α-helix to β-sheet	584:657	The incorporation of CNC restricted water mobility and facilitated conformation conversion of the secondary structure from α-helix to β-sheet.
31888844	3	36	theme	water	553:557	arg1	mobility					559:566	CNC restricted water mobility	538:566	CNC restricted water mobility	538:566	The incorporation of CNC restricted water mobility and facilitated conformation conversion of the secondary structure from α-helix to β-sheet.
31888844	5	37	theme	active	814:819	arg1	CNC					794:796	the agglomerated CNC	777:796	the agglomerated CNC	777:796	At higher CNC concentrations, the agglomerated CNC can serve as an active dehydrating agent to absorb moisture in the protein matrixes, which promotes unfolding and cross-linking of the protein molecules.
31888844	5	37	theme	active	814:819	arg1	agent					833:837	an active dehydrating agent	811:837	an active dehydrating agent to absorb moisture in the protein matrixes, which promotes unfolding and cross-linking of the protein molecules	811:949	At higher CNC concentrations, the agglomerated CNC can serve as an active dehydrating agent to absorb moisture in the protein matrixes, which promotes unfolding and cross-linking of the protein molecules.
31888844	6	38	theme	homogeneous	1042:1052	arg1	structure					1058:1066	a compact and homogeneous gel structure	1028:1066	a compact and homogeneous gel structure	1028:1066	Moreover, the active filling effects of CNC contributed to the formation of a compact and homogeneous gel structure.
31888844	1	39	theme	gels	293:296	arg1	microstructure					235:248	microstructure	235:248	microstructure	235:248	Cellulose nanocrystals (CNC) were successfully prepared from wheat bran, and their effects on the gelling properties and microstructure of heat-induced whey protein isolate (WPI) gels were investigated.
31888844	1	39	theme	gels	293:296	arg1	properties					220:229	gelling properties	212:229	gelling properties	212:229	Cellulose nanocrystals (CNC) were successfully prepared from wheat bran, and their effects on the gelling properties and microstructure of heat-induced whey protein isolate (WPI) gels were investigated.
31888844	6	40	theme	active	966:971	arg1	effects					981:987	the active filling effects	962:987	the active filling effects of CNC	962:994	Moreover, the active filling effects of CNC contributed to the formation of a compact and homogeneous gel structure.
31888844	3	41	theme	mobility	559:566	arg1	incorporation					521:533	The incorporation	517:533	The incorporation of CNC restricted water mobility	517:566	The incorporation of CNC restricted water mobility and facilitated conformation conversion of the secondary structure from α-helix to β-sheet.
31888844	5	42	theme	dehydrating	821:831	arg1	CNC					794:796	the agglomerated CNC	777:796	the agglomerated CNC	777:796	At higher CNC concentrations, the agglomerated CNC can serve as an active dehydrating agent to absorb moisture in the protein matrixes, which promotes unfolding and cross-linking of the protein molecules.
31888844	5	42	theme	dehydrating	821:831	arg1	agent					833:837	an active dehydrating agent	811:837	an active dehydrating agent to absorb moisture in the protein matrixes, which promotes unfolding and cross-linking of the protein molecules	811:949	At higher CNC concentrations, the agglomerated CNC can serve as an active dehydrating agent to absorb moisture in the protein matrixes, which promotes unfolding and cross-linking of the protein molecules.
31888844	0	43	theme	cellulose	51:59	arg1	nanocrystals					61:72	cellulose nanocrystals	51:72	cellulose nanocrystals: Gelling properties and microstructure	51:111	Heat-induced whey protein isolate gels improved by cellulose nanocrystals: Gelling properties and microstructure.
31888844	1	44	dep	properties	220:229	arg1	the					208:210	the	208:210	the	208:210	Cellulose nanocrystals (CNC) were successfully prepared from wheat bran, and their effects on the gelling properties and microstructure of heat-induced whey protein isolate (WPI) gels were investigated.
31888844	3	45	theme	CNC	538:540	arg1	mobility					559:566	CNC restricted water mobility	538:566	CNC restricted water mobility	538:566	The incorporation of CNC restricted water mobility and facilitated conformation conversion of the secondary structure from α-helix to β-sheet.
31888844	2	46	theme	gels	439:442	arg1	strength					373:380	gel strength	369:380	gel strength	369:380	The results showed that the water holding capacity, gel strength, viscoelasticity, and thermal stability of the composite gels were improved by increasing the CNC concentration from 0 to 1.0 % (w/v).
31888844	2	46	theme	gels	439:442	arg1	stability					412:420	thermal stability	404:420	thermal stability	404:420	The results showed that the water holding capacity, gel strength, viscoelasticity, and thermal stability of the composite gels were improved by increasing the CNC concentration from 0 to 1.0 % (w/v).
31888844	2	46	theme	gels	439:442	arg1	viscoelasticity					383:397	viscoelasticity	383:397	viscoelasticity	383:397	The results showed that the water holding capacity, gel strength, viscoelasticity, and thermal stability of the composite gels were improved by increasing the CNC concentration from 0 to 1.0 % (w/v).
31888844	2	46	theme	gels	439:442	arg1	capacity					359:366	the water holding capacity	341:366	the water holding capacity	341:366	The results showed that the water holding capacity, gel strength, viscoelasticity, and thermal stability of the composite gels were improved by increasing the CNC concentration from 0 to 1.0 % (w/v).
31888844	3	47	theme	restricted	542:551	arg1	mobility					559:566	CNC restricted water mobility	538:566	CNC restricted water mobility	538:566	The incorporation of CNC restricted water mobility and facilitated conformation conversion of the secondary structure from α-helix to β-sheet.
31888844	3	48	theme	conformation	584:595	arg1	conversion					597:606	conformation conversion	584:606	conformation conversion of the secondary structure from α-helix to β-sheet	584:657	The incorporation of CNC restricted water mobility and facilitated conformation conversion of the secondary structure from α-helix to β-sheet.
31888844	1	49	from	effects	197:203	arg1	microstructure					235:248	microstructure	235:248	microstructure	235:248	Cellulose nanocrystals (CNC) were successfully prepared from wheat bran, and their effects on the gelling properties and microstructure of heat-induced whey protein isolate (WPI) gels were investigated.
31888844	1	49	from	effects	197:203	arg1	properties					220:229	gelling properties	212:229	gelling properties	212:229	Cellulose nanocrystals (CNC) were successfully prepared from wheat bran, and their effects on the gelling properties and microstructure of heat-induced whey protein isolate (WPI) gels were investigated.
31888844	4	50	theme	protein	696:702	arg1	matrixes					704:711	the protein matrixes	692:711	the protein matrixes	692:711	CNC has good compatibility with the protein matrixes at relatively low concentrations.
31888844	1	51	theme	gelling	212:218	arg1	properties					220:229	gelling properties	212:229	gelling properties	212:229	Cellulose nanocrystals (CNC) were successfully prepared from wheat bran, and their effects on the gelling properties and microstructure of heat-induced whey protein isolate (WPI) gels were investigated.
31888844	6	52	theme	CNC	992:994	arg1	effects					981:987	the active filling effects	962:987	the active filling effects of CNC	962:994	Moreover, the active filling effects of CNC contributed to the formation of a compact and homogeneous gel structure.
31888844	3	53	from	α-helix	640:646	arg1	structure					625:633	the secondary structure	611:633	the secondary structure from α-helix	611:646	The incorporation of CNC restricted water mobility and facilitated conformation conversion of the secondary structure from α-helix to β-sheet.
31888844	3	53	from	α-helix	640:646	arg1	conversion					597:606	conformation conversion	584:606	conformation conversion of the secondary structure from α-helix to β-sheet	584:657	The incorporation of CNC restricted water mobility and facilitated conformation conversion of the secondary structure from α-helix to β-sheet.
31888844	2	54	theme	CNC	476:478	arg1	concentration					480:492	the CNC concentration	472:492	the CNC concentration	472:492	The results showed that the water holding capacity, gel strength, viscoelasticity, and thermal stability of the composite gels were improved by increasing the CNC concentration from 0 to 1.0 % (w/v).
31888844	4	55	contain	has	664:666	arg1	CNC					660:662	CNC	660:662	CNC	660:662	CNC has good compatibility with the protein matrixes at relatively low concentrations.
31888844	4	55	contain	has	664:666	arg2	compatibility					673:685	good compatibility	668:685	good compatibility with the protein matrixes	668:711	CNC has good compatibility with the protein matrixes at relatively low concentrations.
31888844	2	56	theme	holding	351:357	arg1	capacity					359:366	the water holding capacity	341:366	the water holding capacity	341:366	The results showed that the water holding capacity, gel strength, viscoelasticity, and thermal stability of the composite gels were improved by increasing the CNC concentration from 0 to 1.0 % (w/v).
31888844	0	57	theme	whey	13:16	arg1	protein					18:24	Heat-induced whey protein	0:24	Heat-induced whey protein	0:24	Heat-induced whey protein isolate gels improved by cellulose nanocrystals: Gelling properties and microstructure.
35515844	4	0	theme	efficient	470:478	arg1	result					489:494	The most efficient swelling result	461:494	The most efficient swelling result (200%)	461:501	The most efficient swelling result (200%) was observed by the sample containing a low fraction of crosslinker.
35515844	4	0	theme	efficient	470:478	arg1	%					500:500	200%	497:500	200%	497:500	The most efficient swelling result (200%) was observed by the sample containing a low fraction of crosslinker.
35515844	10	1	theme	maximum	1364:1370	arg1	amount					1372:1377	its maximum amount	1360:1377	its maximum amount of 85.5%	1360:1386	This release account of the cephradine demonstrated that the release of the drug increased as the time and pH increased, reaching its maximum amount of 85.5% after 7.5 h.
35515844	10	1	theme	maximum	1364:1370	arg1	%					1386:1386	85.5%	1382:1386	85.5%	1382:1386	This release account of the cephradine demonstrated that the release of the drug increased as the time and pH increased, reaching its maximum amount of 85.5% after 7.5 h.
35515844	7	2	theme	hydrogels	850:858	arg1	analysis					834:841	The biodegradation analysis	815:841	The biodegradation analysis of the hydrogels	815:858	The biodegradation analysis of the hydrogels exhibited the break down by various enzymes into small chain polysaccharides that further broke down in the metabolic pathways.
35515844	1	3	theme	crosslinker	228:238	arg1	APTES					272:276	APTES	272:276	APTES	272:276	Kappa carrageenan was used to prepare hydrogels having novel compositions with poly(vinyl alcohol) (PVA) and a crosslinker (3-aminopropyl)triethoxysilane (APTES).
35515844	1	3	theme	crosslinker	228:238	arg1	triethoxysilane					255:269	a crosslinker (3-aminopropyl)triethoxysilane	226:269	a crosslinker (3-aminopropyl)triethoxysilane (APTES)	226:277	Kappa carrageenan was used to prepare hydrogels having novel compositions with poly(vinyl alcohol) (PVA) and a crosslinker (3-aminopropyl)triethoxysilane (APTES).
35515844	0	4	theme	controlled	83:92	arg1	release					94:100	controlled release	83:100	controlled release of cephradine	83:114	Fabrication of novel carrageenan based stimuli responsive injectable hydrogels for controlled release of cephradine.
35515844	1	5	theme	novel	172:176	arg1	compositions					178:189	novel compositions	172:189	novel compositions	172:189	Kappa carrageenan was used to prepare hydrogels having novel compositions with poly(vinyl alcohol) (PVA) and a crosslinker (3-aminopropyl)triethoxysilane (APTES).
35515844	0	6	theme	cephradine	105:114	arg1	release					94:100	controlled release	83:100	controlled release of cephradine	83:114	Fabrication of novel carrageenan based stimuli responsive injectable hydrogels for controlled release of cephradine.
35515844	5	7	theme	different	619:627	arg1	solutions					632:640	different pH solutions	619:640	different pH solutions that made it suitable for drug delivery	619:680	It also showed different swelling responses in different pH solutions that made it suitable for drug delivery.
35515844	9	8	used	used	1134:1137	arg2	Cephradine					1119:1128	Cephradine	1119:1128	Cephradine	1119:1128	Cephradine was used as a model drug and its in vitro release was studied in simulated intestinal fluids (SIF).
35515844	9	8	used	used	1134:1137	arg2	drug					1150:1153	a model drug	1142:1153	a model drug	1142:1153	Cephradine was used as a model drug and its in vitro release was studied in simulated intestinal fluids (SIF).
35515844	1	9	theme	3-aminopropyl	241:253	arg1	APTES					272:276	APTES	272:276	APTES	272:276	Kappa carrageenan was used to prepare hydrogels having novel compositions with poly(vinyl alcohol) (PVA) and a crosslinker (3-aminopropyl)triethoxysilane (APTES).
35515844	1	9	theme	3-aminopropyl	241:253	arg1	triethoxysilane					255:269	a crosslinker (3-aminopropyl)triethoxysilane	226:269	a crosslinker (3-aminopropyl)triethoxysilane (APTES)	226:277	Kappa carrageenan was used to prepare hydrogels having novel compositions with poly(vinyl alcohol) (PVA) and a crosslinker (3-aminopropyl)triethoxysilane (APTES).
35515844	8	10	theme	hydrogel	1017:1024	arg1	samples					1026:1032	all the hydrogel samples	1009:1032	all the hydrogel samples	1009:1032	It was revealed that all the hydrogel samples showed strong antibacterial activity against S. aureus and a little against E. coli.
35515844	5	11	theme	pH	629:630	arg1	solutions					632:640	different pH solutions	619:640	different pH solutions that made it suitable for drug delivery	619:680	It also showed different swelling responses in different pH solutions that made it suitable for drug delivery.
35515844	7	12	theme	small	909:913	arg1	polysaccharides					921:935	small chain polysaccharides	909:935	small chain polysaccharides that further broke down in the metabolic pathways	909:985	The biodegradation analysis of the hydrogels exhibited the break down by various enzymes into small chain polysaccharides that further broke down in the metabolic pathways.
35515844	4	13	theme	low	543:545	arg1	fraction					547:554	a low fraction	541:554	a low fraction of crosslinker	541:569	The most efficient swelling result (200%) was observed by the sample containing a low fraction of crosslinker.
35515844	1	14	contain	having	165:170	arg1	hydrogels					155:163	hydrogels	155:163	hydrogels having novel compositions with poly(vinyl alcohol) (PVA) and a crosslinker (3-aminopropyl)triethoxysilane (APTES)	155:277	Kappa carrageenan was used to prepare hydrogels having novel compositions with poly(vinyl alcohol) (PVA) and a crosslinker (3-aminopropyl)triethoxysilane (APTES).
35515844	1	14	contain	having	165:170	arg2	compositions					178:189	novel compositions	172:189	novel compositions	172:189	Kappa carrageenan was used to prepare hydrogels having novel compositions with poly(vinyl alcohol) (PVA) and a crosslinker (3-aminopropyl)triethoxysilane (APTES).
35515844	10	15	theme	%	1386:1386	arg1	amount					1372:1377	its maximum amount	1360:1377	its maximum amount of 85.5%	1360:1386	This release account of the cephradine demonstrated that the release of the drug increased as the time and pH increased, reaching its maximum amount of 85.5% after 7.5 h.
35515844	10	15	theme	%	1386:1386	arg1	%					1386:1386	85.5%	1382:1386	85.5%	1382:1386	This release account of the cephradine demonstrated that the release of the drug increased as the time and pH increased, reaching its maximum amount of 85.5% after 7.5 h.
35515844	6	16	theme	crosslinker	754:764	arg1	amount					766:771	crosslinker amount	754:771	crosslinker amount	754:771	Thermogravimetric analysis (TGA) illustrated that with the increase in crosslinker amount, the stability of hydrogel was increased.
35515844	7	17	theme	biodegradation	819:832	arg1	analysis					834:841	The biodegradation analysis	815:841	The biodegradation analysis of the hydrogels	815:858	The biodegradation analysis of the hydrogels exhibited the break down by various enzymes into small chain polysaccharides that further broke down in the metabolic pathways.
35515844	0	18	theme	responsive	47:56	arg1	hydrogels					69:77	responsive injectable hydrogels	47:77	responsive injectable hydrogels	47:77	Fabrication of novel carrageenan based stimuli responsive injectable hydrogels for controlled release of cephradine.
35515844	9	19	dep	in	1163:1164	arg1	vitro					1166:1170	vitro	1166:1170	vitro	1166:1170	Cephradine was used as a model drug and its in vitro release was studied in simulated intestinal fluids (SIF).
35515844	3	20	theme	swelling	353:360	arg1	behavior					362:369	The swelling behavior	349:369	The swelling behavior of hydrogels	349:382	The swelling behavior of hydrogels was studied under different conditions of pH and electrolytic aqueous media.
35515844	5	21	theme	different	587:595	arg1	responses					606:614	different swelling responses	587:614	different swelling responses	587:614	It also showed different swelling responses in different pH solutions that made it suitable for drug delivery.
35515844	5	22	theme	swelling	597:604	arg1	responses					606:614	different swelling responses	587:614	different swelling responses	587:614	It also showed different swelling responses in different pH solutions that made it suitable for drug delivery.
35515844	1	23	used	used	139:142	arg2	carrageenan					123:133	Kappa carrageenan	117:133	Kappa carrageenan	117:133	Kappa carrageenan was used to prepare hydrogels having novel compositions with poly(vinyl alcohol) (PVA) and a crosslinker (3-aminopropyl)triethoxysilane (APTES).
35515844	3	24	theme	different	402:410	arg1	conditions					412:421	different conditions	402:421	different conditions of pH and electrolytic aqueous media	402:458	The swelling behavior of hydrogels was studied under different conditions of pH and electrolytic aqueous media.
35515844	0	25	theme	carrageenan	21:31	arg1	stimuli					39:45	novel carrageenan based stimuli	15:45	novel carrageenan based stimuli	15:45	Fabrication of novel carrageenan based stimuli responsive injectable hydrogels for controlled release of cephradine.
35515844	8	26	theme	strong	1041:1046	arg1	activity					1062:1069	strong antibacterial activity	1041:1069	strong antibacterial activity against S. aureus and a little against E. coli	1041:1116	It was revealed that all the hydrogel samples showed strong antibacterial activity against S. aureus and a little against E. coli.
35515844	7	27	theme	chain	915:919	arg1	polysaccharides					921:935	small chain polysaccharides	909:935	small chain polysaccharides that further broke down in the metabolic pathways	909:985	The biodegradation analysis of the hydrogels exhibited the break down by various enzymes into small chain polysaccharides that further broke down in the metabolic pathways.
35515844	2	28	dep	structure	309:317	arg1	the					305:307	the	305:307	the	305:307	FTIR was used to confirm the structure and composition of hydrogels.
35515844	1	29	theme	vinyl	201:205	arg1	poly					196:199	poly	196:199	poly(vinyl alcohol) (PVA)	196:220	Kappa carrageenan was used to prepare hydrogels having novel compositions with poly(vinyl alcohol) (PVA) and a crosslinker (3-aminopropyl)triethoxysilane (APTES).
35515844	1	29	theme	vinyl	201:205	arg1	alcohol					207:213	vinyl alcohol	201:213	vinyl alcohol	201:213	Kappa carrageenan was used to prepare hydrogels having novel compositions with poly(vinyl alcohol) (PVA) and a crosslinker (3-aminopropyl)triethoxysilane (APTES).
35515844	0	30	theme	novel	15:19	arg1	stimuli					39:45	novel carrageenan based stimuli	15:45	novel carrageenan based stimuli	15:45	Fabrication of novel carrageenan based stimuli responsive injectable hydrogels for controlled release of cephradine.
35515844	1	31	theme	Kappa	117:121	arg1	carrageenan					123:133	Kappa carrageenan	117:133	Kappa carrageenan	117:133	Kappa carrageenan was used to prepare hydrogels having novel compositions with poly(vinyl alcohol) (PVA) and a crosslinker (3-aminopropyl)triethoxysilane (APTES).
35515844	10	32	theme	release	1235:1241	arg1	account					1243:1249	This release account	1230:1249	This release account of the cephradine	1230:1267	This release account of the cephradine demonstrated that the release of the drug increased as the time and pH increased, reaching its maximum amount of 85.5% after 7.5 h.
35515844	3	33	theme	aqueous	446:452	arg1	media					454:458	electrolytic aqueous media	433:458	electrolytic aqueous media	433:458	The swelling behavior of hydrogels was studied under different conditions of pH and electrolytic aqueous media.
35515844	0	34	theme	stimuli	39:45	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of novel carrageenan based stimuli	0:45	Fabrication of novel carrageenan based stimuli responsive injectable hydrogels for controlled release of cephradine.
35515844	5	35	theme	drug	668:671	arg1	delivery					673:680	drug delivery	668:680	drug delivery	668:680	It also showed different swelling responses in different pH solutions that made it suitable for drug delivery.
35515844	3	36	theme	pH	426:427	arg1	conditions					412:421	different conditions	402:421	different conditions of pH and electrolytic aqueous media	402:458	The swelling behavior of hydrogels was studied under different conditions of pH and electrolytic aqueous media.
35515844	0	37	theme	based	33:37	arg1	stimuli					39:45	novel carrageenan based stimuli	15:45	novel carrageenan based stimuli	15:45	Fabrication of novel carrageenan based stimuli responsive injectable hydrogels for controlled release of cephradine.
35515844	8	38	theme	antibacterial	1048:1060	arg1	activity					1062:1069	strong antibacterial activity	1041:1069	strong antibacterial activity against S. aureus and a little against E. coli	1041:1116	It was revealed that all the hydrogel samples showed strong antibacterial activity against S. aureus and a little against E. coli.
35515844	6	39	from	increase	742:749	arg1	amount					766:771	crosslinker amount	754:771	crosslinker amount	754:771	Thermogravimetric analysis (TGA) illustrated that with the increase in crosslinker amount, the stability of hydrogel was increased.
35515844	0	40	theme	injectable	58:67	arg1	hydrogels					69:77	responsive injectable hydrogels	47:77	responsive injectable hydrogels	47:77	Fabrication of novel carrageenan based stimuli responsive injectable hydrogels for controlled release of cephradine.
35515844	4	41	contain	containing	530:539	arg2	fraction					547:554	a low fraction	541:554	a low fraction of crosslinker	541:569	The most efficient swelling result (200%) was observed by the sample containing a low fraction of crosslinker.
35515844	4	41	contain	containing	530:539	arg1	sample					523:528	the sample	519:528	the sample containing a low fraction of crosslinker	519:569	The most efficient swelling result (200%) was observed by the sample containing a low fraction of crosslinker.
35515844	9	42	theme	in	1163:1164	arg1	release					1172:1178	its in vitro release	1159:1178	its in vitro release	1159:1178	Cephradine was used as a model drug and its in vitro release was studied in simulated intestinal fluids (SIF).
35515844	3	43	theme	hydrogels	374:382	arg1	behavior					362:369	The swelling behavior	349:369	The swelling behavior of hydrogels	349:382	The swelling behavior of hydrogels was studied under different conditions of pH and electrolytic aqueous media.
35515844	9	44	theme	simulated	1195:1203	arg1	SIF					1224:1226	SIF	1224:1226	SIF	1224:1226	Cephradine was used as a model drug and its in vitro release was studied in simulated intestinal fluids (SIF).
35515844	9	44	theme	simulated	1195:1203	arg1	fluids					1216:1221	simulated intestinal fluids	1195:1221	simulated intestinal fluids (SIF)	1195:1227	Cephradine was used as a model drug and its in vitro release was studied in simulated intestinal fluids (SIF).
35515844	10	45	theme	cephradine	1258:1267	arg1	account					1243:1249	This release account	1230:1249	This release account of the cephradine	1230:1267	This release account of the cephradine demonstrated that the release of the drug increased as the time and pH increased, reaching its maximum amount of 85.5% after 7.5 h.
35515844	7	46	theme	break	874:878	arg1	down					880:883	the break down	870:883	the break down by various enzymes into small chain polysaccharides that further broke down in the metabolic pathways	870:985	The biodegradation analysis of the hydrogels exhibited the break down by various enzymes into small chain polysaccharides that further broke down in the metabolic pathways.
35515844	7	47	theme	various	888:894	arg1	enzymes					896:902	various enzymes	888:902	various enzymes	888:902	The biodegradation analysis of the hydrogels exhibited the break down by various enzymes into small chain polysaccharides that further broke down in the metabolic pathways.
35515844	6	48	theme	hydrogel	791:798	arg1	stability					778:786	the stability	774:786	the stability of hydrogel	774:798	Thermogravimetric analysis (TGA) illustrated that with the increase in crosslinker amount, the stability of hydrogel was increased.
35515844	7	49	theme	metabolic	968:976	arg1	pathways					978:985	the metabolic pathways	964:985	the metabolic pathways	964:985	The biodegradation analysis of the hydrogels exhibited the break down by various enzymes into small chain polysaccharides that further broke down in the metabolic pathways.
35515844	3	50	theme	media	454:458	arg1	conditions					412:421	different conditions	402:421	different conditions of pH and electrolytic aqueous media	402:458	The swelling behavior of hydrogels was studied under different conditions of pH and electrolytic aqueous media.
35515844	4	51	theme	crosslinker	559:569	arg1	fraction					547:554	a low fraction	541:554	a low fraction of crosslinker	541:569	The most efficient swelling result (200%) was observed by the sample containing a low fraction of crosslinker.
35515844	9	52	theme	intestinal	1205:1214	arg1	SIF					1224:1226	SIF	1224:1226	SIF	1224:1226	Cephradine was used as a model drug and its in vitro release was studied in simulated intestinal fluids (SIF).
35515844	9	52	theme	intestinal	1205:1214	arg1	fluids					1216:1221	simulated intestinal fluids	1195:1221	simulated intestinal fluids (SIF)	1195:1227	Cephradine was used as a model drug and its in vitro release was studied in simulated intestinal fluids (SIF).
35515844	9	53	theme	model	1144:1148	arg1	Cephradine					1119:1128	Cephradine	1119:1128	Cephradine	1119:1128	Cephradine was used as a model drug and its in vitro release was studied in simulated intestinal fluids (SIF).
35515844	9	53	theme	model	1144:1148	arg1	drug					1150:1153	a model drug	1142:1153	a model drug	1142:1153	Cephradine was used as a model drug and its in vitro release was studied in simulated intestinal fluids (SIF).
35515844	3	54	theme	electrolytic	433:444	arg1	media					454:458	electrolytic aqueous media	433:458	electrolytic aqueous media	433:458	The swelling behavior of hydrogels was studied under different conditions of pH and electrolytic aqueous media.
35515844	2	55	theme	hydrogels	338:346	arg1	composition					323:333	composition	323:333	composition	323:333	FTIR was used to confirm the structure and composition of hydrogels.
35515844	2	55	theme	hydrogels	338:346	arg1	structure					309:317	structure	309:317	structure	309:317	FTIR was used to confirm the structure and composition of hydrogels.
35515844	2	56	used	used	289:292	arg2	FTIR					280:283	FTIR	280:283	FTIR	280:283	FTIR was used to confirm the structure and composition of hydrogels.
35515844	10	57	theme	drug	1306:1309	arg1	release					1291:1297	the release	1287:1297	the release of the drug	1287:1309	This release account of the cephradine demonstrated that the release of the drug increased as the time and pH increased, reaching its maximum amount of 85.5% after 7.5 h.
35515844	4	58	theme	swelling	480:487	arg1	result					489:494	The most efficient swelling result	461:494	The most efficient swelling result (200%)	461:501	The most efficient swelling result (200%) was observed by the sample containing a low fraction of crosslinker.
35515844	4	58	theme	swelling	480:487	arg1	%					500:500	200%	497:500	200%	497:500	The most efficient swelling result (200%) was observed by the sample containing a low fraction of crosslinker.
35515844	6	59	theme	Thermogravimetric	683:699	arg1	analysis					701:708	Thermogravimetric analysis	683:708	Thermogravimetric analysis (TGA)	683:714	Thermogravimetric analysis (TGA) illustrated that with the increase in crosslinker amount, the stability of hydrogel was increased.
35515844	6	59	theme	Thermogravimetric	683:699	arg1	TGA					711:713	TGA	711:713	TGA	711:713	Thermogravimetric analysis (TGA) illustrated that with the increase in crosslinker amount, the stability of hydrogel was increased.
29355812	3	0	theme	increased	709:717	arg1	permeability					728:739	increased membrane permeability	709:739	increased membrane permeability	709:739	These changes appeared very rapidly after dilution, and were linked to increased membrane permeability and loss in cell viability.
29355812	6	1	theme	fluorescence	1267:1278	arg1	parameters					1280:1289	fluorescence parameters	1267:1289	fluorescence parameters using flow cytometry	1267:1310	Thus, stabilizing the bacterial outer membrane is essential for precise and unbiased measurements of fluorescence parameters using flow cytometry.
29355812	2	2	theme	fluorescent	371:381	arg1	proteins					383:390	constitutively expressed fluorescent proteins	346:390	constitutively expressed fluorescent proteins in Escherichia coli K-12 strain MG1655	346:429	Using a variety of constitutively expressed fluorescent proteins in Escherichia coli K-12 strain MG1655, we found strong artifactual changes in fluorescence levels after dilution into the commonly used flow cytometry buffer phosphate-buffered saline (PBS) and two other buffer solutions, Tris-HCl and M9 salts.
29355812	1	3	theme	bacterial	244:252	arg1	physiology					254:263	bacterial physiology	244:263	bacterial physiology	244:263	Buffers are essential for diluting bacterial cultures for flow cytometry analysis in order to study bacterial physiology and gene expression parameters based on fluorescence signals.
29355812	5	4	theme	fluorescence	1099:1110	arg1	signals					1112:1118	preserved fluorescence signals	1089:1118	preserved fluorescence signals	1089:1118	Supplementing the buffers with divalent cations responsible for outer membrane stability, Mg2+ and Ca2+, preserved fluorescence signals, membrane integrity and viability of E. coli.
29355812	2	5	from	variety	335:341	arg1	strain					417:422	Escherichia coli K-12 strain MG1655	395:429	Escherichia coli K-12 strain MG1655	395:429	Using a variety of constitutively expressed fluorescent proteins in Escherichia coli K-12 strain MG1655, we found strong artifactual changes in fluorescence levels after dilution into the commonly used flow cytometry buffer phosphate-buffered saline (PBS) and two other buffer solutions, Tris-HCl and M9 salts.
29355812	2	6	theme	expressed	361:369	arg1	proteins					383:390	constitutively expressed fluorescent proteins	346:390	constitutively expressed fluorescent proteins in Escherichia coli K-12 strain MG1655	346:429	Using a variety of constitutively expressed fluorescent proteins in Escherichia coli K-12 strain MG1655, we found strong artifactual changes in fluorescence levels after dilution into the commonly used flow cytometry buffer phosphate-buffered saline (PBS) and two other buffer solutions, Tris-HCl and M9 salts.
29355812	1	7	theme	physiology	254:263	arg1	parameters					285:294	bacterial physiology and gene expression parameters	244:294	bacterial physiology and gene expression parameters based on fluorescence signals	244:324	Buffers are essential for diluting bacterial cultures for flow cytometry analysis in order to study bacterial physiology and gene expression parameters based on fluorescence signals.
29355812	2	8	theme	phosphate-buffered	551:568	arg1	PBS					578:580	PBS	578:580	PBS	578:580	Using a variety of constitutively expressed fluorescent proteins in Escherichia coli K-12 strain MG1655, we found strong artifactual changes in fluorescence levels after dilution into the commonly used flow cytometry buffer phosphate-buffered saline (PBS) and two other buffer solutions, Tris-HCl and M9 salts.
29355812	2	8	theme	phosphate-buffered	551:568	arg1	saline					570:575	phosphate-buffered saline	551:575	phosphate-buffered saline (PBS)	551:581	Using a variety of constitutively expressed fluorescent proteins in Escherichia coli K-12 strain MG1655, we found strong artifactual changes in fluorescence levels after dilution into the commonly used flow cytometry buffer phosphate-buffered saline (PBS) and two other buffer solutions, Tris-HCl and M9 salts.
29355812	0	9	from	Lack	0:3	arg1	cytometry					24:32	flow cytometry	19:32	flow cytometry	19:32	Lack of cations in flow cytometry buffers affect fluorescence signals by reducing membrane stability and viability of Escherichia coli strains.
29355812	4	10	theme	E.	865:866	arg1	strains					833:839	several different E. coli strains	807:839	several different E. coli strains	807:839	We observed buffer-related effects in several different E. coli strains, K-12, C and W, but not E. coli B, which can be partially explained by differences in lipopolysaccharide (LPS) and outer membrane composition.
29355812	4	10	theme	E.	865:866	arg1	B					873:873	E. coli B	865:873	E. coli B	865:873	We observed buffer-related effects in several different E. coli strains, K-12, C and W, but not E. coli B, which can be partially explained by differences in lipopolysaccharide (LPS) and outer membrane composition.
29355812	4	11	from	differences	912:922	arg1	composition					971:981	outer membrane composition	956:981	outer membrane composition	956:981	We observed buffer-related effects in several different E. coli strains, K-12, C and W, but not E. coli B, which can be partially explained by differences in lipopolysaccharide (LPS) and outer membrane composition.
29355812	4	11	from	differences	912:922	arg1	LPS					947:949	LPS	947:949	LPS	947:949	We observed buffer-related effects in several different E. coli strains, K-12, C and W, but not E. coli B, which can be partially explained by differences in lipopolysaccharide (LPS) and outer membrane composition.
29355812	4	11	from	differences	912:922	arg1	lipopolysaccharide					927:944	lipopolysaccharide	927:944	lipopolysaccharide (LPS)	927:950	We observed buffer-related effects in several different E. coli strains, K-12, C and W, but not E. coli B, which can be partially explained by differences in lipopolysaccharide (LPS) and outer membrane composition.
29355812	0	12	theme	membrane	82:89	arg1	stability					91:99	membrane stability	82:99	membrane stability	82:99	Lack of cations in flow cytometry buffers affect fluorescence signals by reducing membrane stability and viability of Escherichia coli strains.
29355812	6	13	theme	unbiased	1242:1249	arg1	measurements					1251:1262	precise and unbiased measurements	1230:1262	precise and unbiased measurements of fluorescence parameters using flow cytometry	1230:1310	Thus, stabilizing the bacterial outer membrane is essential for precise and unbiased measurements of fluorescence parameters using flow cytometry.
29355812	3	14	from	loss	745:748	arg1	viability					758:766	cell viability	753:766	cell viability	753:766	These changes appeared very rapidly after dilution, and were linked to increased membrane permeability and loss in cell viability.
29355812	4	15	theme	outer	956:960	arg1	composition					971:981	outer membrane composition	956:981	outer membrane composition	956:981	We observed buffer-related effects in several different E. coli strains, K-12, C and W, but not E. coli B, which can be partially explained by differences in lipopolysaccharide (LPS) and outer membrane composition.
29355812	1	16	theme	gene	269:272	arg1	expression					274:283	gene expression	269:283	gene expression	269:283	Buffers are essential for diluting bacterial cultures for flow cytometry analysis in order to study bacterial physiology and gene expression parameters based on fluorescence signals.
29355812	2	17	theme	cytometry	534:542	arg1	buffer					544:549	the commonly used flow cytometry buffer phosphate-buffered saline (PBS) and two other buffer solutions, Tris-HCl and M9 salts	511:635	buffer	544:549	Using a variety of constitutively expressed fluorescent proteins in Escherichia coli K-12 strain MG1655, we found strong artifactual changes in fluorescence levels after dilution into the commonly used flow cytometry buffer phosphate-buffered saline (PBS) and two other buffer solutions, Tris-HCl and M9 salts.
29355812	6	18	theme	flow	1297:1300	arg1	cytometry					1302:1310	flow cytometry	1297:1310	flow cytometry	1297:1310	Thus, stabilizing the bacterial outer membrane is essential for precise and unbiased measurements of fluorescence parameters using flow cytometry.
29355812	2	19	theme	coli	407:410	arg1	strain					417:422	Escherichia coli K-12 strain MG1655	395:429	Escherichia coli K-12 strain MG1655	395:429	Using a variety of constitutively expressed fluorescent proteins in Escherichia coli K-12 strain MG1655, we found strong artifactual changes in fluorescence levels after dilution into the commonly used flow cytometry buffer phosphate-buffered saline (PBS) and two other buffer solutions, Tris-HCl and M9 salts.
29355812	2	20	theme	flow	529:532	arg1	cytometry					534:542	the commonly used flow cytometry	511:542	the commonly used flow cytometry	511:542	Using a variety of constitutively expressed fluorescent proteins in Escherichia coli K-12 strain MG1655, we found strong artifactual changes in fluorescence levels after dilution into the commonly used flow cytometry buffer phosphate-buffered saline (PBS) and two other buffer solutions, Tris-HCl and M9 salts.
29355812	3	21	theme	membrane	719:726	arg1	permeability					728:739	increased membrane permeability	709:739	increased membrane permeability	709:739	These changes appeared very rapidly after dilution, and were linked to increased membrane permeability and loss in cell viability.
29355812	6	22	theme	precise	1230:1236	arg1	measurements					1251:1262	precise and unbiased measurements	1230:1262	precise and unbiased measurements of fluorescence parameters using flow cytometry	1230:1310	Thus, stabilizing the bacterial outer membrane is essential for precise and unbiased measurements of fluorescence parameters using flow cytometry.
29355812	4	23	theme	buffer-related	781:794	arg1	effects					796:802	buffer-related effects	781:802	buffer-related effects in several different E. coli strains, K-12, C and W, but not E. coli B, which can be partially explained by differences in lipopolysaccharide (LPS) and outer membrane composition	781:981	We observed buffer-related effects in several different E. coli strains, K-12, C and W, but not E. coli B, which can be partially explained by differences in lipopolysaccharide (LPS) and outer membrane composition.
29355812	2	24	theme	K-12	412:415	arg1	strain					417:422	Escherichia coli K-12 strain MG1655	395:429	Escherichia coli K-12 strain MG1655	395:429	Using a variety of constitutively expressed fluorescent proteins in Escherichia coli K-12 strain MG1655, we found strong artifactual changes in fluorescence levels after dilution into the commonly used flow cytometry buffer phosphate-buffered saline (PBS) and two other buffer solutions, Tris-HCl and M9 salts.
29355812	4	25	theme	coli	868:871	arg1	strains					833:839	several different E. coli strains	807:839	several different E. coli strains	807:839	We observed buffer-related effects in several different E. coli strains, K-12, C and W, but not E. coli B, which can be partially explained by differences in lipopolysaccharide (LPS) and outer membrane composition.
29355812	4	25	theme	coli	868:871	arg1	B					873:873	E. coli B	865:873	E. coli B	865:873	We observed buffer-related effects in several different E. coli strains, K-12, C and W, but not E. coli B, which can be partially explained by differences in lipopolysaccharide (LPS) and outer membrane composition.
29355812	1	26	theme	bacterial	179:187	arg1	cultures					189:196	bacterial cultures	179:196	bacterial cultures for flow cytometry analysis in order to study bacterial physiology and gene expression parameters based on fluorescence signals	179:324	Buffers are essential for diluting bacterial cultures for flow cytometry analysis in order to study bacterial physiology and gene expression parameters based on fluorescence signals.
29355812	3	27	from	permeability	728:739	arg1	viability					758:766	cell viability	753:766	cell viability	753:766	These changes appeared very rapidly after dilution, and were linked to increased membrane permeability and loss in cell viability.
29355812	1	28	theme	expression	274:283	arg1	parameters					285:294	bacterial physiology and gene expression parameters	244:294	bacterial physiology and gene expression parameters based on fluorescence signals	244:324	Buffers are essential for diluting bacterial cultures for flow cytometry analysis in order to study bacterial physiology and gene expression parameters based on fluorescence signals.
29355812	2	29	theme	M9	628:629	arg1	salts					631:635	M9 salts	628:635	M9 salts	628:635	Using a variety of constitutively expressed fluorescent proteins in Escherichia coli K-12 strain MG1655, we found strong artifactual changes in fluorescence levels after dilution into the commonly used flow cytometry buffer phosphate-buffered saline (PBS) and two other buffer solutions, Tris-HCl and M9 salts.
29355812	2	29	theme	M9	628:629	arg1	solutions					604:612	two other buffer solutions	587:612	two other buffer solutions	587:612	Using a variety of constitutively expressed fluorescent proteins in Escherichia coli K-12 strain MG1655, we found strong artifactual changes in fluorescence levels after dilution into the commonly used flow cytometry buffer phosphate-buffered saline (PBS) and two other buffer solutions, Tris-HCl and M9 salts.
29355812	2	30	theme	Escherichia	395:405	arg1	strain					417:422	Escherichia coli K-12 strain MG1655	395:429	Escherichia coli K-12 strain MG1655	395:429	Using a variety of constitutively expressed fluorescent proteins in Escherichia coli K-12 strain MG1655, we found strong artifactual changes in fluorescence levels after dilution into the commonly used flow cytometry buffer phosphate-buffered saline (PBS) and two other buffer solutions, Tris-HCl and M9 salts.
29355812	2	31	theme	artifactual	448:458	arg1	changes					460:466	strong artifactual changes	441:466	strong artifactual changes	441:466	Using a variety of constitutively expressed fluorescent proteins in Escherichia coli K-12 strain MG1655, we found strong artifactual changes in fluorescence levels after dilution into the commonly used flow cytometry buffer phosphate-buffered saline (PBS) and two other buffer solutions, Tris-HCl and M9 salts.
29355812	0	32	theme	Escherichia	118:128	arg1	strains					135:141	Escherichia coli strains	118:141	Escherichia coli strains	118:141	Lack of cations in flow cytometry buffers affect fluorescence signals by reducing membrane stability and viability of Escherichia coli strains.
29355812	2	33	from	proteins	383:390	arg1	strain					417:422	Escherichia coli K-12 strain MG1655	395:429	Escherichia coli K-12 strain MG1655	395:429	Using a variety of constitutively expressed fluorescent proteins in Escherichia coli K-12 strain MG1655, we found strong artifactual changes in fluorescence levels after dilution into the commonly used flow cytometry buffer phosphate-buffered saline (PBS) and two other buffer solutions, Tris-HCl and M9 salts.
29355812	4	34	from	effects	796:802	arg1	C					848:848	C	848:848	C	848:848	We observed buffer-related effects in several different E. coli strains, K-12, C and W, but not E. coli B, which can be partially explained by differences in lipopolysaccharide (LPS) and outer membrane composition.
29355812	4	34	from	effects	796:802	arg1	strains					833:839	several different E. coli strains	807:839	several different E. coli strains	807:839	We observed buffer-related effects in several different E. coli strains, K-12, C and W, but not E. coli B, which can be partially explained by differences in lipopolysaccharide (LPS) and outer membrane composition.
29355812	4	34	from	effects	796:802	arg1	B					873:873	E. coli B	865:873	E. coli B	865:873	We observed buffer-related effects in several different E. coli strains, K-12, C and W, but not E. coli B, which can be partially explained by differences in lipopolysaccharide (LPS) and outer membrane composition.
29355812	4	34	from	effects	796:802	arg1	K-12					842:845	K-12	842:845	K-12	842:845	We observed buffer-related effects in several different E. coli strains, K-12, C and W, but not E. coli B, which can be partially explained by differences in lipopolysaccharide (LPS) and outer membrane composition.
29355812	3	35	attach	linked	699:704	arg2	changes					644:650	These changes	638:650	These changes	638:650	These changes appeared very rapidly after dilution, and were linked to increased membrane permeability and loss in cell viability.
29355812	3	35	attach	linked	699:704	arg1	permeability					728:739	increased membrane permeability	709:739	increased membrane permeability	709:739	These changes appeared very rapidly after dilution, and were linked to increased membrane permeability and loss in cell viability.
29355812	3	35	attach	linked	699:704	arg1	loss					745:748	loss	745:748	loss in cell viability	745:766	These changes appeared very rapidly after dilution, and were linked to increased membrane permeability and loss in cell viability.
29355812	0	36	theme	cations	8:14	arg1	Lack					0:3	Lack	0:3	Lack of cations in flow cytometry	0:32	Lack of cations in flow cytometry buffers affect fluorescence signals by reducing membrane stability and viability of Escherichia coli strains.
29355812	5	37	theme	coli	1160:1163	arg1	signals					1112:1118	preserved fluorescence signals	1089:1118	preserved fluorescence signals	1089:1118	Supplementing the buffers with divalent cations responsible for outer membrane stability, Mg2+ and Ca2+, preserved fluorescence signals, membrane integrity and viability of E. coli.
29355812	5	37	theme	coli	1160:1163	arg1	Mg2+					1074:1077	Mg2+	1074:1077	Mg2+	1074:1077	Supplementing the buffers with divalent cations responsible for outer membrane stability, Mg2+ and Ca2+, preserved fluorescence signals, membrane integrity and viability of E. coli.
29355812	5	37	theme	coli	1160:1163	arg1	Ca2+					1083:1086	Ca2+	1083:1086	Ca2+	1083:1086	Supplementing the buffers with divalent cations responsible for outer membrane stability, Mg2+ and Ca2+, preserved fluorescence signals, membrane integrity and viability of E. coli.
29355812	5	37	theme	coli	1160:1163	arg1	integrity					1130:1138	membrane integrity	1121:1138	membrane integrity	1121:1138	Supplementing the buffers with divalent cations responsible for outer membrane stability, Mg2+ and Ca2+, preserved fluorescence signals, membrane integrity and viability of E. coli.
29355812	5	37	theme	coli	1160:1163	arg1	viability					1144:1152	viability	1144:1152	viability of E. coli	1144:1163	Supplementing the buffers with divalent cations responsible for outer membrane stability, Mg2+ and Ca2+, preserved fluorescence signals, membrane integrity and viability of E. coli.
29355812	5	37	theme	coli	1160:1163	arg1	stability					1063:1071	outer membrane stability	1048:1071	outer membrane stability	1048:1071	Supplementing the buffers with divalent cations responsible for outer membrane stability, Mg2+ and Ca2+, preserved fluorescence signals, membrane integrity and viability of E. coli.
29355812	5	38	theme	outer	1048:1052	arg1	stability					1063:1071	outer membrane stability	1048:1071	outer membrane stability	1048:1071	Supplementing the buffers with divalent cations responsible for outer membrane stability, Mg2+ and Ca2+, preserved fluorescence signals, membrane integrity and viability of E. coli.
29355812	1	39	theme	flow	202:205	arg1	analysis					217:224	flow cytometry analysis	202:224	flow cytometry analysis in order to study bacterial physiology and gene expression parameters based on fluorescence signals	202:324	Buffers are essential for diluting bacterial cultures for flow cytometry analysis in order to study bacterial physiology and gene expression parameters based on fluorescence signals.
29355812	4	40	theme	membrane	962:969	arg1	composition					971:981	outer membrane composition	956:981	outer membrane composition	956:981	We observed buffer-related effects in several different E. coli strains, K-12, C and W, but not E. coli B, which can be partially explained by differences in lipopolysaccharide (LPS) and outer membrane composition.
29355812	3	41	theme	cell	753:756	arg1	viability					758:766	cell viability	753:766	cell viability	753:766	These changes appeared very rapidly after dilution, and were linked to increased membrane permeability and loss in cell viability.
29355812	0	42	theme	strains	135:141	arg1	stability					91:99	membrane stability	82:99	membrane stability	82:99	Lack of cations in flow cytometry buffers affect fluorescence signals by reducing membrane stability and viability of Escherichia coli strains.
29355812	0	42	theme	strains	135:141	arg1	viability					105:113	viability	105:113	viability	105:113	Lack of cations in flow cytometry buffers affect fluorescence signals by reducing membrane stability and viability of Escherichia coli strains.
29355812	5	43	theme	membrane	1054:1061	arg1	stability					1063:1071	outer membrane stability	1048:1071	outer membrane stability	1048:1071	Supplementing the buffers with divalent cations responsible for outer membrane stability, Mg2+ and Ca2+, preserved fluorescence signals, membrane integrity and viability of E. coli.
29355812	1	44	theme	cytometry	207:215	arg1	analysis					217:224	flow cytometry analysis	202:224	flow cytometry analysis in order to study bacterial physiology and gene expression parameters based on fluorescence signals	202:324	Buffers are essential for diluting bacterial cultures for flow cytometry analysis in order to study bacterial physiology and gene expression parameters based on fluorescence signals.
29355812	0	45	theme	flow	19:22	arg1	cytometry					24:32	flow cytometry	19:32	flow cytometry	19:32	Lack of cations in flow cytometry buffers affect fluorescence signals by reducing membrane stability and viability of Escherichia coli strains.
29355812	1	46	theme	fluorescence	305:316	arg1	signals					318:324	fluorescence signals	305:324	fluorescence signals	305:324	Buffers are essential for diluting bacterial cultures for flow cytometry analysis in order to study bacterial physiology and gene expression parameters based on fluorescence signals.
29355812	0	47	theme	coli	130:133	arg1	strains					135:141	Escherichia coli strains	118:141	Escherichia coli strains	118:141	Lack of cations in flow cytometry buffers affect fluorescence signals by reducing membrane stability and viability of Escherichia coli strains.
29355812	4	48	theme	coli	828:831	arg1	C					848:848	C	848:848	C	848:848	We observed buffer-related effects in several different E. coli strains, K-12, C and W, but not E. coli B, which can be partially explained by differences in lipopolysaccharide (LPS) and outer membrane composition.
29355812	4	48	theme	coli	828:831	arg1	strains					833:839	several different E. coli strains	807:839	several different E. coli strains	807:839	We observed buffer-related effects in several different E. coli strains, K-12, C and W, but not E. coli B, which can be partially explained by differences in lipopolysaccharide (LPS) and outer membrane composition.
29355812	4	48	theme	coli	828:831	arg1	B					873:873	E. coli B	865:873	E. coli B	865:873	We observed buffer-related effects in several different E. coli strains, K-12, C and W, but not E. coli B, which can be partially explained by differences in lipopolysaccharide (LPS) and outer membrane composition.
29355812	4	48	theme	coli	828:831	arg1	K-12					842:845	K-12	842:845	K-12	842:845	We observed buffer-related effects in several different E. coli strains, K-12, C and W, but not E. coli B, which can be partially explained by differences in lipopolysaccharide (LPS) and outer membrane composition.
29355812	5	49	theme	membrane	1121:1128	arg1	integrity					1130:1138	membrane integrity	1121:1138	membrane integrity	1121:1138	Supplementing the buffers with divalent cations responsible for outer membrane stability, Mg2+ and Ca2+, preserved fluorescence signals, membrane integrity and viability of E. coli.
29355812	2	50	theme	buffer	597:602	arg1	Tris-HCl					615:622	Tris-HCl	615:622	Tris-HCl	615:622	Using a variety of constitutively expressed fluorescent proteins in Escherichia coli K-12 strain MG1655, we found strong artifactual changes in fluorescence levels after dilution into the commonly used flow cytometry buffer phosphate-buffered saline (PBS) and two other buffer solutions, Tris-HCl and M9 salts.
29355812	2	50	theme	buffer	597:602	arg1	salts					631:635	M9 salts	628:635	M9 salts	628:635	Using a variety of constitutively expressed fluorescent proteins in Escherichia coli K-12 strain MG1655, we found strong artifactual changes in fluorescence levels after dilution into the commonly used flow cytometry buffer phosphate-buffered saline (PBS) and two other buffer solutions, Tris-HCl and M9 salts.
29355812	2	50	theme	buffer	597:602	arg1	solutions					604:612	two other buffer solutions	587:612	two other buffer solutions	587:612	Using a variety of constitutively expressed fluorescent proteins in Escherichia coli K-12 strain MG1655, we found strong artifactual changes in fluorescence levels after dilution into the commonly used flow cytometry buffer phosphate-buffered saline (PBS) and two other buffer solutions, Tris-HCl and M9 salts.
29355812	6	51	theme	outer	1198:1202	arg1	essential					1216:1224	essential	1216:1224	essential	1216:1224	Thus, stabilizing the bacterial outer membrane is essential for precise and unbiased measurements of fluorescence parameters using flow cytometry.
29355812	6	51	theme	outer	1198:1202	arg1	membrane					1204:1211	the bacterial outer membrane	1184:1211	stabilizing the bacterial outer membrane	1172:1211	Thus, stabilizing the bacterial outer membrane is essential for precise and unbiased measurements of fluorescence parameters using flow cytometry.
29355812	2	52	theme	fluorescence	471:482	arg1	levels					484:489	fluorescence levels	471:489	fluorescence levels	471:489	Using a variety of constitutively expressed fluorescent proteins in Escherichia coli K-12 strain MG1655, we found strong artifactual changes in fluorescence levels after dilution into the commonly used flow cytometry buffer phosphate-buffered saline (PBS) and two other buffer solutions, Tris-HCl and M9 salts.
29355812	2	53	theme	other	591:595	arg1	Tris-HCl					615:622	Tris-HCl	615:622	Tris-HCl	615:622	Using a variety of constitutively expressed fluorescent proteins in Escherichia coli K-12 strain MG1655, we found strong artifactual changes in fluorescence levels after dilution into the commonly used flow cytometry buffer phosphate-buffered saline (PBS) and two other buffer solutions, Tris-HCl and M9 salts.
29355812	2	53	theme	other	591:595	arg1	salts					631:635	M9 salts	628:635	M9 salts	628:635	Using a variety of constitutively expressed fluorescent proteins in Escherichia coli K-12 strain MG1655, we found strong artifactual changes in fluorescence levels after dilution into the commonly used flow cytometry buffer phosphate-buffered saline (PBS) and two other buffer solutions, Tris-HCl and M9 salts.
29355812	2	53	theme	other	591:595	arg1	solutions					604:612	two other buffer solutions	587:612	two other buffer solutions	587:612	Using a variety of constitutively expressed fluorescent proteins in Escherichia coli K-12 strain MG1655, we found strong artifactual changes in fluorescence levels after dilution into the commonly used flow cytometry buffer phosphate-buffered saline (PBS) and two other buffer solutions, Tris-HCl and M9 salts.
29355812	5	54	theme	divalent	1015:1022	arg1	cations					1024:1030	divalent cations	1015:1030	divalent cations responsible for outer membrane stability, Mg2+ and Ca2+, preserved fluorescence signals, membrane integrity and viability of E. coli	1015:1163	Supplementing the buffers with divalent cations responsible for outer membrane stability, Mg2+ and Ca2+, preserved fluorescence signals, membrane integrity and viability of E. coli.
29355812	6	55	theme	bacterial	1188:1196	arg1	essential					1216:1224	essential	1216:1224	essential	1216:1224	Thus, stabilizing the bacterial outer membrane is essential for precise and unbiased measurements of fluorescence parameters using flow cytometry.
29355812	6	55	theme	bacterial	1188:1196	arg1	membrane					1204:1211	the bacterial outer membrane	1184:1211	stabilizing the bacterial outer membrane	1172:1211	Thus, stabilizing the bacterial outer membrane is essential for precise and unbiased measurements of fluorescence parameters using flow cytometry.
29355812	2	56	from	strain	417:422	arg1	proteins					383:390	constitutively expressed fluorescent proteins	346:390	constitutively expressed fluorescent proteins in Escherichia coli K-12 strain MG1655	346:429	Using a variety of constitutively expressed fluorescent proteins in Escherichia coli K-12 strain MG1655, we found strong artifactual changes in fluorescence levels after dilution into the commonly used flow cytometry buffer phosphate-buffered saline (PBS) and two other buffer solutions, Tris-HCl and M9 salts.
29355812	2	56	from	strain	417:422	arg1	variety					335:341	a variety	333:341	a variety of constitutively expressed fluorescent proteins in Escherichia coli K-12 strain MG1655	333:429	Using a variety of constitutively expressed fluorescent proteins in Escherichia coli K-12 strain MG1655, we found strong artifactual changes in fluorescence levels after dilution into the commonly used flow cytometry buffer phosphate-buffered saline (PBS) and two other buffer solutions, Tris-HCl and M9 salts.
29355812	4	57	theme	different	815:823	arg1	C					848:848	C	848:848	C	848:848	We observed buffer-related effects in several different E. coli strains, K-12, C and W, but not E. coli B, which can be partially explained by differences in lipopolysaccharide (LPS) and outer membrane composition.
29355812	4	57	theme	different	815:823	arg1	strains					833:839	several different E. coli strains	807:839	several different E. coli strains	807:839	We observed buffer-related effects in several different E. coli strains, K-12, C and W, but not E. coli B, which can be partially explained by differences in lipopolysaccharide (LPS) and outer membrane composition.
29355812	4	57	theme	different	815:823	arg1	B					873:873	E. coli B	865:873	E. coli B	865:873	We observed buffer-related effects in several different E. coli strains, K-12, C and W, but not E. coli B, which can be partially explained by differences in lipopolysaccharide (LPS) and outer membrane composition.
29355812	4	57	theme	different	815:823	arg1	K-12					842:845	K-12	842:845	K-12	842:845	We observed buffer-related effects in several different E. coli strains, K-12, C and W, but not E. coli B, which can be partially explained by differences in lipopolysaccharide (LPS) and outer membrane composition.
29355812	4	58	theme	several	807:813	arg1	C					848:848	C	848:848	C	848:848	We observed buffer-related effects in several different E. coli strains, K-12, C and W, but not E. coli B, which can be partially explained by differences in lipopolysaccharide (LPS) and outer membrane composition.
29355812	4	58	theme	several	807:813	arg1	strains					833:839	several different E. coli strains	807:839	several different E. coli strains	807:839	We observed buffer-related effects in several different E. coli strains, K-12, C and W, but not E. coli B, which can be partially explained by differences in lipopolysaccharide (LPS) and outer membrane composition.
29355812	4	58	theme	several	807:813	arg1	B					873:873	E. coli B	865:873	E. coli B	865:873	We observed buffer-related effects in several different E. coli strains, K-12, C and W, but not E. coli B, which can be partially explained by differences in lipopolysaccharide (LPS) and outer membrane composition.
29355812	4	58	theme	several	807:813	arg1	K-12					842:845	K-12	842:845	K-12	842:845	We observed buffer-related effects in several different E. coli strains, K-12, C and W, but not E. coli B, which can be partially explained by differences in lipopolysaccharide (LPS) and outer membrane composition.
29355812	6	59	theme	parameters	1280:1289	arg1	measurements					1251:1262	precise and unbiased measurements	1230:1262	precise and unbiased measurements of fluorescence parameters using flow cytometry	1230:1310	Thus, stabilizing the bacterial outer membrane is essential for precise and unbiased measurements of fluorescence parameters using flow cytometry.
29355812	5	60	theme	responsible	1032:1042	arg1	cations					1024:1030	divalent cations	1015:1030	divalent cations responsible for outer membrane stability, Mg2+ and Ca2+, preserved fluorescence signals, membrane integrity and viability of E. coli	1015:1163	Supplementing the buffers with divalent cations responsible for outer membrane stability, Mg2+ and Ca2+, preserved fluorescence signals, membrane integrity and viability of E. coli.
29355812	6	61	theme	stabilizing	1172:1182	arg1	essential					1216:1224	essential	1216:1224	essential	1216:1224	Thus, stabilizing the bacterial outer membrane is essential for precise and unbiased measurements of fluorescence parameters using flow cytometry.
29355812	6	61	theme	stabilizing	1172:1182	arg1	membrane					1204:1211	the bacterial outer membrane	1184:1211	stabilizing the bacterial outer membrane	1172:1211	Thus, stabilizing the bacterial outer membrane is essential for precise and unbiased measurements of fluorescence parameters using flow cytometry.
29355812	2	62	dep	buffer	544:549	arg1	PBS					578:580	PBS	578:580	PBS	578:580	Using a variety of constitutively expressed fluorescent proteins in Escherichia coli K-12 strain MG1655, we found strong artifactual changes in fluorescence levels after dilution into the commonly used flow cytometry buffer phosphate-buffered saline (PBS) and two other buffer solutions, Tris-HCl and M9 salts.
29355812	2	62	dep	buffer	544:549	arg1	saline					570:575	phosphate-buffered saline	551:575	phosphate-buffered saline (PBS)	551:581	Using a variety of constitutively expressed fluorescent proteins in Escherichia coli K-12 strain MG1655, we found strong artifactual changes in fluorescence levels after dilution into the commonly used flow cytometry buffer phosphate-buffered saline (PBS) and two other buffer solutions, Tris-HCl and M9 salts.
29355812	2	63	theme	used	524:527	arg1	cytometry					534:542	the commonly used flow cytometry	511:542	the commonly used flow cytometry	511:542	Using a variety of constitutively expressed fluorescent proteins in Escherichia coli K-12 strain MG1655, we found strong artifactual changes in fluorescence levels after dilution into the commonly used flow cytometry buffer phosphate-buffered saline (PBS) and two other buffer solutions, Tris-HCl and M9 salts.
29355812	4	64	theme	E.	825:826	arg1	C					848:848	C	848:848	C	848:848	We observed buffer-related effects in several different E. coli strains, K-12, C and W, but not E. coli B, which can be partially explained by differences in lipopolysaccharide (LPS) and outer membrane composition.
29355812	4	64	theme	E.	825:826	arg1	strains					833:839	several different E. coli strains	807:839	several different E. coli strains	807:839	We observed buffer-related effects in several different E. coli strains, K-12, C and W, but not E. coli B, which can be partially explained by differences in lipopolysaccharide (LPS) and outer membrane composition.
29355812	4	64	theme	E.	825:826	arg1	B					873:873	E. coli B	865:873	E. coli B	865:873	We observed buffer-related effects in several different E. coli strains, K-12, C and W, but not E. coli B, which can be partially explained by differences in lipopolysaccharide (LPS) and outer membrane composition.
29355812	4	64	theme	E.	825:826	arg1	K-12					842:845	K-12	842:845	K-12	842:845	We observed buffer-related effects in several different E. coli strains, K-12, C and W, but not E. coli B, which can be partially explained by differences in lipopolysaccharide (LPS) and outer membrane composition.
29355812	0	65	theme	fluorescence	49:60	arg1	signals					62:68	fluorescence signals	49:68	fluorescence signals	49:68	Lack of cations in flow cytometry buffers affect fluorescence signals by reducing membrane stability and viability of Escherichia coli strains.
29355812	5	66	theme	preserved	1089:1097	arg1	signals					1112:1118	preserved fluorescence signals	1089:1118	preserved fluorescence signals	1089:1118	Supplementing the buffers with divalent cations responsible for outer membrane stability, Mg2+ and Ca2+, preserved fluorescence signals, membrane integrity and viability of E. coli.
29355812	2	67	theme	strong	441:446	arg1	changes					460:466	strong artifactual changes	441:466	strong artifactual changes	441:466	Using a variety of constitutively expressed fluorescent proteins in Escherichia coli K-12 strain MG1655, we found strong artifactual changes in fluorescence levels after dilution into the commonly used flow cytometry buffer phosphate-buffered saline (PBS) and two other buffer solutions, Tris-HCl and M9 salts.
29355812	2	68	theme	proteins	383:390	arg1	proteins					383:390	constitutively expressed fluorescent proteins	346:390	constitutively expressed fluorescent proteins in Escherichia coli K-12 strain MG1655	346:429	Using a variety of constitutively expressed fluorescent proteins in Escherichia coli K-12 strain MG1655, we found strong artifactual changes in fluorescence levels after dilution into the commonly used flow cytometry buffer phosphate-buffered saline (PBS) and two other buffer solutions, Tris-HCl and M9 salts.
29355812	2	68	theme	proteins	383:390	arg1	variety					335:341	a variety	333:341	a variety of constitutively expressed fluorescent proteins in Escherichia coli K-12 strain MG1655	333:429	Using a variety of constitutively expressed fluorescent proteins in Escherichia coli K-12 strain MG1655, we found strong artifactual changes in fluorescence levels after dilution into the commonly used flow cytometry buffer phosphate-buffered saline (PBS) and two other buffer solutions, Tris-HCl and M9 salts.
29498616	2	0	theme	flagellated	114:124	arg1	strain					181:186	A Gram-stain-positive, flagellated, catalase- and cytochrome c oxidase-positive bacterial strain	91:186	A Gram-stain-positive, flagellated, catalase- and cytochrome c oxidase-positive bacterial strain	91:186	A Gram-stain-positive, flagellated, catalase- and cytochrome c oxidase-positive bacterial strain, designated S20-100T, was isolated from alpine forest soil.
29498616	3	1	dep	%	336:336	arg1	w/v					339:341	w/v	339:341	w/v	339:341	Growth occurred at a temperature range of 0-30 °C, at pH 6-9 and in the presence of 0-1 % (w/v) NaCl.
29498616	9	2	theme	name	1271:1274	arg1	sp					1299:1300	the name Marmoricola silvestris sp	1267:1300	the name Marmoricola silvestris sp	1267:1300	Combined data of phylogenetic, phenotypic and chemotaxonomic analyses demonstrated that strain S20-100T represents a novel species of the genus Marmoricola, for which the name Marmoricola silvestris sp.
29498616	11	3	theme	type	1325:1328	arg1	S20-100T					1340:1347	S20-100T	1340:1347	S20-100T (=DSM 104694T=LMG 30008T)	1340:1373	The type strain is S20-100T (=DSM 104694T=LMG 30008T).
29498616	11	3	theme	type	1325:1328	arg1	strain					1330:1335	The type strain	1321:1335	The type strain	1321:1335	The type strain is S20-100T (=DSM 104694T=LMG 30008T).
29498616	9	4	theme	phylogenetic	1117:1128	arg1	analyses					1161:1168	phylogenetic, phenotypic and chemotaxonomic analyses	1117:1168	phylogenetic, phenotypic and chemotaxonomic analyses	1117:1168	Combined data of phylogenetic, phenotypic and chemotaxonomic analyses demonstrated that strain S20-100T represents a novel species of the genus Marmoricola, for which the name Marmoricola silvestris sp.
29498616	6	5	from	lipids	948:953	arg1	amounts					964:970	lower amounts	958:970	lower amounts	958:970	The strain contained MK-8(H4) as the predominant isoprenoid quinone and diphosphatidylglycerol, phosphatidylglycerol, four unidentified phospholipids and three unidentified lipids in lower amounts.
29498616	5	6	theme	S20-100T	655:662	arg1	peptidoglycan					631:643	The cell-wall peptidoglycan	617:643	The cell-wall peptidoglycan of strain S20-100T	617:662	The cell-wall peptidoglycan of strain S20-100T contained ll-diaminopimelic acid (ll-Dpm) as the diagnostic diamino acid and was of the type A3γ ll-Dpm - Gly.
29498616	2	7	theme	Gram-stain-positive	93:111	arg1	strain					181:186	A Gram-stain-positive, flagellated, catalase- and cytochrome c oxidase-positive bacterial strain	91:186	A Gram-stain-positive, flagellated, catalase- and cytochrome c oxidase-positive bacterial strain	91:186	A Gram-stain-positive, flagellated, catalase- and cytochrome c oxidase-positive bacterial strain, designated S20-100T, was isolated from alpine forest soil.
29498616	2	8	attach	isolated	214:221	arg1	soil					242:245	alpine forest soil	228:245	alpine forest soil	228:245	A Gram-stain-positive, flagellated, catalase- and cytochrome c oxidase-positive bacterial strain, designated S20-100T, was isolated from alpine forest soil.
29498616	2	8	attach	isolated	214:221	arg2	strain					181:186	A Gram-stain-positive, flagellated, catalase- and cytochrome c oxidase-positive bacterial strain	91:186	A Gram-stain-positive, flagellated, catalase- and cytochrome c oxidase-positive bacterial strain	91:186	A Gram-stain-positive, flagellated, catalase- and cytochrome c oxidase-positive bacterial strain, designated S20-100T, was isolated from alpine forest soil.
29498616	3	9	theme	0-1 	332:335	arg1	%					336:336	%	336:336	%	336:336	Growth occurred at a temperature range of 0-30 °C, at pH 6-9 and in the presence of 0-1 % (w/v) NaCl.
29498616	4	10	theme	Marmoricola	526:536	arg1	097T					557:560	Marmoricola ginsengisoli Gsoil 097T	526:560	Marmoricola ginsengisoli Gsoil 097T (98.4 %)	526:569	Phylogenetic analysis based on 16S rRNA gene sequence showed that strain S20-100T was related to the genus Marmoricola and had the highest 16S rRNA gene sequence similarity to Marmoricola ginsengisoli Gsoil 097T (98.4 %) and Marmoricola solisilvae KIS18-7T (98.3 %).
29498616	4	10	theme	Marmoricola	526:536	arg1	%					568:568	98.4 %	563:568	98.4 %	563:568	Phylogenetic analysis based on 16S rRNA gene sequence showed that strain S20-100T was related to the genus Marmoricola and had the highest 16S rRNA gene sequence similarity to Marmoricola ginsengisoli Gsoil 097T (98.4 %) and Marmoricola solisilvae KIS18-7T (98.3 %).
29498616	5	11	theme	ll-diaminopimelic	674:690	arg1	ll-Dpm					698:703	ll-Dpm	698:703	ll-Dpm	698:703	The cell-wall peptidoglycan of strain S20-100T contained ll-diaminopimelic acid (ll-Dpm) as the diagnostic diamino acid and was of the type A3γ ll-Dpm - Gly.
29498616	5	11	theme	ll-diaminopimelic	674:690	arg1	acid					692:695	ll-diaminopimelic acid	674:695	ll-diaminopimelic acid (ll-Dpm)	674:704	The cell-wall peptidoglycan of strain S20-100T contained ll-diaminopimelic acid (ll-Dpm) as the diagnostic diamino acid and was of the type A3γ ll-Dpm - Gly.
29498616	5	11	theme	ll-diaminopimelic	674:690	arg1	acid					732:735	the diagnostic diamino acid	709:735	the diagnostic diamino acid	709:735	The cell-wall peptidoglycan of strain S20-100T contained ll-diaminopimelic acid (ll-Dpm) as the diagnostic diamino acid and was of the type A3γ ll-Dpm - Gly.
29498616	4	12	theme	strain	416:421	arg1	S20-100T					423:430	strain S20-100T	416:430	strain S20-100T	416:430	Phylogenetic analysis based on 16S rRNA gene sequence showed that strain S20-100T was related to the genus Marmoricola and had the highest 16S rRNA gene sequence similarity to Marmoricola ginsengisoli Gsoil 097T (98.4 %) and Marmoricola solisilvae KIS18-7T (98.3 %).
29498616	6	13	from	phospholipids	911:923	arg1	amounts					964:970	lower amounts	958:970	lower amounts	958:970	The strain contained MK-8(H4) as the predominant isoprenoid quinone and diphosphatidylglycerol, phosphatidylglycerol, four unidentified phospholipids and three unidentified lipids in lower amounts.
29498616	4	14	theme	Phylogenetic	350:361	arg1	analysis					363:370	Phylogenetic analysis	350:370	Phylogenetic analysis based on 16S rRNA gene sequence	350:402	Phylogenetic analysis based on 16S rRNA gene sequence showed that strain S20-100T was related to the genus Marmoricola and had the highest 16S rRNA gene sequence similarity to Marmoricola ginsengisoli Gsoil 097T (98.4 %) and Marmoricola solisilvae KIS18-7T (98.3 %).
29498616	8	15	theme	DNA	1069:1071	arg1	G+C content					1073:1083	The genomic DNA G+C content	1057:1083	The genomic DNA G+C content	1057:1083	The genomic DNA G+C content was 66.2 mol%.
29498616	8	15	theme	DNA	1069:1071	arg1	%					1097:1097	66.2 mol%	1089:1097	66.2 mol%	1089:1097	The genomic DNA G+C content was 66.2 mol%.
29498616	6	16	theme	unidentified	898:909	arg1	phospholipids					911:923	four unidentified phospholipids	893:923	four unidentified phospholipids	893:923	The strain contained MK-8(H4) as the predominant isoprenoid quinone and diphosphatidylglycerol, phosphatidylglycerol, four unidentified phospholipids and three unidentified lipids in lower amounts.
29498616	6	16	theme	unidentified	898:909	arg1	quinone					835:841	the predominant isoprenoid quinone	808:841	the predominant isoprenoid quinone	808:841	The strain contained MK-8(H4) as the predominant isoprenoid quinone and diphosphatidylglycerol, phosphatidylglycerol, four unidentified phospholipids and three unidentified lipids in lower amounts.
29498616	5	17	contain	contained	664:672	arg1	peptidoglycan					631:643	The cell-wall peptidoglycan	617:643	The cell-wall peptidoglycan of strain S20-100T	617:662	The cell-wall peptidoglycan of strain S20-100T contained ll-diaminopimelic acid (ll-Dpm) as the diagnostic diamino acid and was of the type A3γ ll-Dpm - Gly.
29498616	5	17	contain	contained	664:672	arg2	acid					732:735	the diagnostic diamino acid	709:735	the diagnostic diamino acid	709:735	The cell-wall peptidoglycan of strain S20-100T contained ll-diaminopimelic acid (ll-Dpm) as the diagnostic diamino acid and was of the type A3γ ll-Dpm - Gly.
29498616	5	17	contain	contained	664:672	arg2	acid					692:695	ll-diaminopimelic acid	674:695	ll-diaminopimelic acid (ll-Dpm)	674:704	The cell-wall peptidoglycan of strain S20-100T contained ll-diaminopimelic acid (ll-Dpm) as the diagnostic diamino acid and was of the type A3γ ll-Dpm - Gly.
29498616	5	17	contain	contained	664:672	arg2	ll-Dpm					698:703	ll-Dpm	698:703	ll-Dpm	698:703	The cell-wall peptidoglycan of strain S20-100T contained ll-diaminopimelic acid (ll-Dpm) as the diagnostic diamino acid and was of the type A3γ ll-Dpm - Gly.
29498616	1	18	theme	alpine	71:76	arg1	soil					85:88	alpine forest soil	71:88	alpine forest soil	71:88	nov., a novel actinobacterium isolated from alpine forest soil.
29498616	4	19	theme	solisilvae	587:596	arg1	KIS18-7T					598:605	Marmoricola solisilvae KIS18-7T	575:605	Marmoricola solisilvae KIS18-7T (98.3 %)	575:614	Phylogenetic analysis based on 16S rRNA gene sequence showed that strain S20-100T was related to the genus Marmoricola and had the highest 16S rRNA gene sequence similarity to Marmoricola ginsengisoli Gsoil 097T (98.4 %) and Marmoricola solisilvae KIS18-7T (98.3 %).
29498616	4	19	theme	solisilvae	587:596	arg1	%					613:613	98.3 %	608:613	98.3 %	608:613	Phylogenetic analysis based on 16S rRNA gene sequence showed that strain S20-100T was related to the genus Marmoricola and had the highest 16S rRNA gene sequence similarity to Marmoricola ginsengisoli Gsoil 097T (98.4 %) and Marmoricola solisilvae KIS18-7T (98.3 %).
29498616	0	20	theme	silvestris	12:21	arg1	sp					23:24	Marmoricola silvestris sp	0:24	Marmoricola silvestris sp.	0:25	Marmoricola silvestris sp.
29498616	2	21	theme	catalase-	127:135	arg1	strain					181:186	A Gram-stain-positive, flagellated, catalase- and cytochrome c oxidase-positive bacterial strain	91:186	A Gram-stain-positive, flagellated, catalase- and cytochrome c oxidase-positive bacterial strain	91:186	A Gram-stain-positive, flagellated, catalase- and cytochrome c oxidase-positive bacterial strain, designated S20-100T, was isolated from alpine forest soil.
29498616	7	22	theme	major	977:981	arg1	acids					998:1002	The major cellular fatty acids	973:1002	The major cellular fatty acids (>10 %)	973:1010	The major cellular fatty acids (>10 %) were iso-C16 : 0, C17 : 1ω6c and C18 : 1ω9c.
29498616	7	22	theme	major	977:981	arg1	%					1009:1009	>10 %	1005:1009	>10 %	1005:1009	The major cellular fatty acids (>10 %) were iso-C16 : 0, C17 : 1ω6c and C18 : 1ω9c.
29498616	7	22	theme	major	977:981	arg1	iso-C16 					1017:1024	iso-C16 	1017:1024	iso-C16 	1017:1024	The major cellular fatty acids (>10 %) were iso-C16 : 0, C17 : 1ω6c and C18 : 1ω9c.
29498616	9	23	theme	strain	1188:1193	arg1	S20-100T					1195:1202	strain S20-100T	1188:1202	strain S20-100T	1188:1202	Combined data of phylogenetic, phenotypic and chemotaxonomic analyses demonstrated that strain S20-100T represents a novel species of the genus Marmoricola, for which the name Marmoricola silvestris sp.
29498616	1	24	theme	forest	78:83	arg1	soil					85:88	alpine forest soil	71:88	alpine forest soil	71:88	nov., a novel actinobacterium isolated from alpine forest soil.
29498616	2	25	theme	cytochrome	141:150	arg1	c					152:152	cytochrome c	141:152	cytochrome c	141:152	A Gram-stain-positive, flagellated, catalase- and cytochrome c oxidase-positive bacterial strain, designated S20-100T, was isolated from alpine forest soil.
29498616	0	26	theme	Marmoricola	0:10	arg1	sp					23:24	Marmoricola silvestris sp	0:24	Marmoricola silvestris sp.	0:25	Marmoricola silvestris sp.
29498616	4	27	theme	rRNA	493:496	arg1	similarity					512:521	the highest 16S rRNA gene sequence similarity	477:521	the highest 16S rRNA gene sequence similarity to Marmoricola ginsengisoli Gsoil 097T (98.4 %) and Marmoricola solisilvae KIS18-7T (98.3 %)	477:614	Phylogenetic analysis based on 16S rRNA gene sequence showed that strain S20-100T was related to the genus Marmoricola and had the highest 16S rRNA gene sequence similarity to Marmoricola ginsengisoli Gsoil 097T (98.4 %) and Marmoricola solisilvae KIS18-7T (98.3 %).
29498616	9	28	theme	chemotaxonomic	1146:1159	arg1	analyses					1161:1168	phylogenetic, phenotypic and chemotaxonomic analyses	1117:1168	phylogenetic, phenotypic and chemotaxonomic analyses	1117:1168	Combined data of phylogenetic, phenotypic and chemotaxonomic analyses demonstrated that strain S20-100T represents a novel species of the genus Marmoricola, for which the name Marmoricola silvestris sp.
29498616	9	29	theme	analyses	1161:1168	arg1	data					1109:1112	Combined data	1100:1112	Combined data of phylogenetic, phenotypic and chemotaxonomic analyses	1100:1168	Combined data of phylogenetic, phenotypic and chemotaxonomic analyses demonstrated that strain S20-100T represents a novel species of the genus Marmoricola, for which the name Marmoricola silvestris sp.
29498616	9	30	theme	silvestris	1288:1297	arg1	sp					1299:1300	the name Marmoricola silvestris sp	1267:1300	the name Marmoricola silvestris sp	1267:1300	Combined data of phylogenetic, phenotypic and chemotaxonomic analyses demonstrated that strain S20-100T represents a novel species of the genus Marmoricola, for which the name Marmoricola silvestris sp.
29498616	3	31	theme	%	336:336	arg1	NaCl					344:347	0-1 % (w/v) NaCl	332:347	0-1 % (w/v) NaCl	332:347	Growth occurred at a temperature range of 0-30 °C, at pH 6-9 and in the presence of 0-1 % (w/v) NaCl.
29498616	4	32	theme	16S	489:491	arg1	similarity					512:521	the highest 16S rRNA gene sequence similarity	477:521	the highest 16S rRNA gene sequence similarity to Marmoricola ginsengisoli Gsoil 097T (98.4 %) and Marmoricola solisilvae KIS18-7T (98.3 %)	477:614	Phylogenetic analysis based on 16S rRNA gene sequence showed that strain S20-100T was related to the genus Marmoricola and had the highest 16S rRNA gene sequence similarity to Marmoricola ginsengisoli Gsoil 097T (98.4 %) and Marmoricola solisilvae KIS18-7T (98.3 %).
29498616	3	33	theme	temperature	269:279	arg1	range					281:285	a temperature range	267:285	a temperature range of 0-30 °C	267:296	Growth occurred at a temperature range of 0-30 °C, at pH 6-9 and in the presence of 0-1 % (w/v) NaCl.
29498616	5	34	theme	diamino	724:730	arg1	acid					732:735	the diagnostic diamino acid	709:735	the diagnostic diamino acid	709:735	The cell-wall peptidoglycan of strain S20-100T contained ll-diaminopimelic acid (ll-Dpm) as the diagnostic diamino acid and was of the type A3γ ll-Dpm - Gly.
29498616	5	34	theme	diamino	724:730	arg1	acid					692:695	ll-diaminopimelic acid	674:695	ll-diaminopimelic acid (ll-Dpm)	674:704	The cell-wall peptidoglycan of strain S20-100T contained ll-diaminopimelic acid (ll-Dpm) as the diagnostic diamino acid and was of the type A3γ ll-Dpm - Gly.
29498616	5	35	theme	type	752:755	arg1	ll-Dpm					761:766	the type A3γ ll-Dpm	748:766	the type A3γ ll-Dpm	748:766	The cell-wall peptidoglycan of strain S20-100T contained ll-diaminopimelic acid (ll-Dpm) as the diagnostic diamino acid and was of the type A3γ ll-Dpm - Gly.
29498616	7	36	dep	 0	1026:1027	arg1	 1ω6c					1035:1039	 1ω6c	1035:1039	 1ω6c	1035:1039	The major cellular fatty acids (>10 %) were iso-C16 : 0, C17 : 1ω6c and C18 : 1ω9c.
29498616	7	36	dep	 0	1026:1027	arg1	 1ω9c					1050:1054	 1ω9c	1050:1054	 1ω9c	1050:1054	The major cellular fatty acids (>10 %) were iso-C16 : 0, C17 : 1ω6c and C18 : 1ω9c.
29498616	7	36	dep	 0	1026:1027	arg1	C18 					1045:1048	C18 	1045:1048	C18 	1045:1048	The major cellular fatty acids (>10 %) were iso-C16 : 0, C17 : 1ω6c and C18 : 1ω9c.
29498616	4	37	theme	sequence	503:510	arg1	similarity					512:521	the highest 16S rRNA gene sequence similarity	477:521	the highest 16S rRNA gene sequence similarity to Marmoricola ginsengisoli Gsoil 097T (98.4 %) and Marmoricola solisilvae KIS18-7T (98.3 %)	477:614	Phylogenetic analysis based on 16S rRNA gene sequence showed that strain S20-100T was related to the genus Marmoricola and had the highest 16S rRNA gene sequence similarity to Marmoricola ginsengisoli Gsoil 097T (98.4 %) and Marmoricola solisilvae KIS18-7T (98.3 %).
29498616	9	38	theme	Combined	1100:1107	arg1	data					1109:1112	Combined data	1100:1112	Combined data of phylogenetic, phenotypic and chemotaxonomic analyses	1100:1168	Combined data of phylogenetic, phenotypic and chemotaxonomic analyses demonstrated that strain S20-100T represents a novel species of the genus Marmoricola, for which the name Marmoricola silvestris sp.
29498616	5	39	theme	A3γ	757:759	arg1	ll-Dpm					761:766	the type A3γ ll-Dpm	748:766	the type A3γ ll-Dpm	748:766	The cell-wall peptidoglycan of strain S20-100T contained ll-diaminopimelic acid (ll-Dpm) as the diagnostic diamino acid and was of the type A3γ ll-Dpm - Gly.
29498616	8	40	theme	66.2 mol	1089:1096	arg1	G+C content					1073:1083	The genomic DNA G+C content	1057:1083	The genomic DNA G+C content	1057:1083	The genomic DNA G+C content was 66.2 mol%.
29498616	8	40	theme	66.2 mol	1089:1096	arg1	%					1097:1097	66.2 mol%	1089:1097	66.2 mol%	1089:1097	The genomic DNA G+C content was 66.2 mol%.
29498616	4	41	theme	gene	498:501	arg1	similarity					512:521	the highest 16S rRNA gene sequence similarity	477:521	the highest 16S rRNA gene sequence similarity to Marmoricola ginsengisoli Gsoil 097T (98.4 %) and Marmoricola solisilvae KIS18-7T (98.3 %)	477:614	Phylogenetic analysis based on 16S rRNA gene sequence showed that strain S20-100T was related to the genus Marmoricola and had the highest 16S rRNA gene sequence similarity to Marmoricola ginsengisoli Gsoil 097T (98.4 %) and Marmoricola solisilvae KIS18-7T (98.3 %).
29498616	7	42	theme	cellular	983:990	arg1	acids					998:1002	The major cellular fatty acids	973:1002	The major cellular fatty acids (>10 %)	973:1010	The major cellular fatty acids (>10 %) were iso-C16 : 0, C17 : 1ω6c and C18 : 1ω9c.
29498616	7	42	theme	cellular	983:990	arg1	%					1009:1009	>10 %	1005:1009	>10 %	1005:1009	The major cellular fatty acids (>10 %) were iso-C16 : 0, C17 : 1ω6c and C18 : 1ω9c.
29498616	7	42	theme	cellular	983:990	arg1	iso-C16 					1017:1024	iso-C16 	1017:1024	iso-C16 	1017:1024	The major cellular fatty acids (>10 %) were iso-C16 : 0, C17 : 1ω6c and C18 : 1ω9c.
29498616	4	43	theme	gene	390:393	arg1	sequence					395:402	16S rRNA gene sequence	381:402	16S rRNA gene sequence	381:402	Phylogenetic analysis based on 16S rRNA gene sequence showed that strain S20-100T was related to the genus Marmoricola and had the highest 16S rRNA gene sequence similarity to Marmoricola ginsengisoli Gsoil 097T (98.4 %) and Marmoricola solisilvae KIS18-7T (98.3 %).
29498616	2	44	theme	forest	235:240	arg1	soil					242:245	alpine forest soil	228:245	alpine forest soil	228:245	A Gram-stain-positive, flagellated, catalase- and cytochrome c oxidase-positive bacterial strain, designated S20-100T, was isolated from alpine forest soil.
29498616	4	45	theme	Marmoricola	575:585	arg1	KIS18-7T					598:605	Marmoricola solisilvae KIS18-7T	575:605	Marmoricola solisilvae KIS18-7T (98.3 %)	575:614	Phylogenetic analysis based on 16S rRNA gene sequence showed that strain S20-100T was related to the genus Marmoricola and had the highest 16S rRNA gene sequence similarity to Marmoricola ginsengisoli Gsoil 097T (98.4 %) and Marmoricola solisilvae KIS18-7T (98.3 %).
29498616	4	45	theme	Marmoricola	575:585	arg1	%					613:613	98.3 %	608:613	98.3 %	608:613	Phylogenetic analysis based on 16S rRNA gene sequence showed that strain S20-100T was related to the genus Marmoricola and had the highest 16S rRNA gene sequence similarity to Marmoricola ginsengisoli Gsoil 097T (98.4 %) and Marmoricola solisilvae KIS18-7T (98.3 %).
29498616	5	46	theme	diagnostic	713:722	arg1	acid					732:735	the diagnostic diamino acid	709:735	the diagnostic diamino acid	709:735	The cell-wall peptidoglycan of strain S20-100T contained ll-diaminopimelic acid (ll-Dpm) as the diagnostic diamino acid and was of the type A3γ ll-Dpm - Gly.
29498616	5	46	theme	diagnostic	713:722	arg1	acid					692:695	ll-diaminopimelic acid	674:695	ll-diaminopimelic acid (ll-Dpm)	674:704	The cell-wall peptidoglycan of strain S20-100T contained ll-diaminopimelic acid (ll-Dpm) as the diagnostic diamino acid and was of the type A3γ ll-Dpm - Gly.
29498616	9	47	theme	Marmoricola	1244:1254	arg1	species					1223:1229	a novel species	1215:1229	a novel species	1215:1229	Combined data of phylogenetic, phenotypic and chemotaxonomic analyses demonstrated that strain S20-100T represents a novel species of the genus Marmoricola, for which the name Marmoricola silvestris sp.
29498616	5	48	theme	strain	648:653	arg1	S20-100T					655:662	strain S20-100T	648:662	strain S20-100T	648:662	The cell-wall peptidoglycan of strain S20-100T contained ll-diaminopimelic acid (ll-Dpm) as the diagnostic diamino acid and was of the type A3γ ll-Dpm - Gly.
29498616	2	49	theme	bacterial	171:179	arg1	strain					181:186	A Gram-stain-positive, flagellated, catalase- and cytochrome c oxidase-positive bacterial strain	91:186	A Gram-stain-positive, flagellated, catalase- and cytochrome c oxidase-positive bacterial strain	91:186	A Gram-stain-positive, flagellated, catalase- and cytochrome c oxidase-positive bacterial strain, designated S20-100T, was isolated from alpine forest soil.
29498616	2	50	theme	alpine	228:233	arg1	soil					242:245	alpine forest soil	228:245	alpine forest soil	228:245	A Gram-stain-positive, flagellated, catalase- and cytochrome c oxidase-positive bacterial strain, designated S20-100T, was isolated from alpine forest soil.
29498616	8	51	theme	genomic	1061:1067	arg1	G+C content					1073:1083	The genomic DNA G+C content	1057:1083	The genomic DNA G+C content	1057:1083	The genomic DNA G+C content was 66.2 mol%.
29498616	8	51	theme	genomic	1061:1067	arg1	%					1097:1097	66.2 mol%	1089:1097	66.2 mol%	1089:1097	The genomic DNA G+C content was 66.2 mol%.
29498616	3	52	theme	NaCl	344:347	arg1	presence					320:327	the presence	316:327	the presence of 0-1 % (w/v) NaCl	316:347	Growth occurred at a temperature range of 0-30 °C, at pH 6-9 and in the presence of 0-1 % (w/v) NaCl.
29498616	2	53	theme	oxidase-positive	154:169	arg1	strain					181:186	A Gram-stain-positive, flagellated, catalase- and cytochrome c oxidase-positive bacterial strain	91:186	A Gram-stain-positive, flagellated, catalase- and cytochrome c oxidase-positive bacterial strain	91:186	A Gram-stain-positive, flagellated, catalase- and cytochrome c oxidase-positive bacterial strain, designated S20-100T, was isolated from alpine forest soil.
29498616	7	54	theme	fatty	992:996	arg1	acids					998:1002	The major cellular fatty acids	973:1002	The major cellular fatty acids (>10 %)	973:1010	The major cellular fatty acids (>10 %) were iso-C16 : 0, C17 : 1ω6c and C18 : 1ω9c.
29498616	7	54	theme	fatty	992:996	arg1	%					1009:1009	>10 %	1005:1009	>10 %	1005:1009	The major cellular fatty acids (>10 %) were iso-C16 : 0, C17 : 1ω6c and C18 : 1ω9c.
29498616	7	54	theme	fatty	992:996	arg1	iso-C16 					1017:1024	iso-C16 	1017:1024	iso-C16 	1017:1024	The major cellular fatty acids (>10 %) were iso-C16 : 0, C17 : 1ω6c and C18 : 1ω9c.
29498616	4	55	theme	genus	451:455	arg1	Marmoricola					457:467	the genus Marmoricola	447:467	the genus Marmoricola	447:467	Phylogenetic analysis based on 16S rRNA gene sequence showed that strain S20-100T was related to the genus Marmoricola and had the highest 16S rRNA gene sequence similarity to Marmoricola ginsengisoli Gsoil 097T (98.4 %) and Marmoricola solisilvae KIS18-7T (98.3 %).
29498616	4	56	theme	highest	481:487	arg1	similarity					512:521	the highest 16S rRNA gene sequence similarity	477:521	the highest 16S rRNA gene sequence similarity to Marmoricola ginsengisoli Gsoil 097T (98.4 %) and Marmoricola solisilvae KIS18-7T (98.3 %)	477:614	Phylogenetic analysis based on 16S rRNA gene sequence showed that strain S20-100T was related to the genus Marmoricola and had the highest 16S rRNA gene sequence similarity to Marmoricola ginsengisoli Gsoil 097T (98.4 %) and Marmoricola solisilvae KIS18-7T (98.3 %).
29498616	5	57	theme	cell-wall	621:629	arg1	peptidoglycan					631:643	The cell-wall peptidoglycan	617:643	The cell-wall peptidoglycan of strain S20-100T	617:662	The cell-wall peptidoglycan of strain S20-100T contained ll-diaminopimelic acid (ll-Dpm) as the diagnostic diamino acid and was of the type A3γ ll-Dpm - Gly.
29498616	4	58	theme	ginsengisoli	538:549	arg1	097T					557:560	Marmoricola ginsengisoli Gsoil 097T	526:560	Marmoricola ginsengisoli Gsoil 097T (98.4 %)	526:569	Phylogenetic analysis based on 16S rRNA gene sequence showed that strain S20-100T was related to the genus Marmoricola and had the highest 16S rRNA gene sequence similarity to Marmoricola ginsengisoli Gsoil 097T (98.4 %) and Marmoricola solisilvae KIS18-7T (98.3 %).
29498616	4	58	theme	ginsengisoli	538:549	arg1	%					568:568	98.4 %	563:568	98.4 %	563:568	Phylogenetic analysis based on 16S rRNA gene sequence showed that strain S20-100T was related to the genus Marmoricola and had the highest 16S rRNA gene sequence similarity to Marmoricola ginsengisoli Gsoil 097T (98.4 %) and Marmoricola solisilvae KIS18-7T (98.3 %).
29498616	6	59	theme	isoprenoid	824:833	arg1	phosphatidylglycerol					871:890	phosphatidylglycerol	871:890	phosphatidylglycerol	871:890	The strain contained MK-8(H4) as the predominant isoprenoid quinone and diphosphatidylglycerol, phosphatidylglycerol, four unidentified phospholipids and three unidentified lipids in lower amounts.
29498616	6	59	theme	isoprenoid	824:833	arg1	MK-8					796:799	MK-8	796:799	MK-8(H4)	796:803	The strain contained MK-8(H4) as the predominant isoprenoid quinone and diphosphatidylglycerol, phosphatidylglycerol, four unidentified phospholipids and three unidentified lipids in lower amounts.
29498616	6	59	theme	isoprenoid	824:833	arg1	lipids					948:953	three unidentified lipids	929:953	three unidentified lipids	929:953	The strain contained MK-8(H4) as the predominant isoprenoid quinone and diphosphatidylglycerol, phosphatidylglycerol, four unidentified phospholipids and three unidentified lipids in lower amounts.
29498616	6	59	theme	isoprenoid	824:833	arg1	quinone					835:841	the predominant isoprenoid quinone	808:841	the predominant isoprenoid quinone	808:841	The strain contained MK-8(H4) as the predominant isoprenoid quinone and diphosphatidylglycerol, phosphatidylglycerol, four unidentified phospholipids and three unidentified lipids in lower amounts.
29498616	6	59	theme	isoprenoid	824:833	arg1	phospholipids					911:923	four unidentified phospholipids	893:923	four unidentified phospholipids	893:923	The strain contained MK-8(H4) as the predominant isoprenoid quinone and diphosphatidylglycerol, phosphatidylglycerol, four unidentified phospholipids and three unidentified lipids in lower amounts.
29498616	9	60	theme	phenotypic	1131:1140	arg1	analyses					1161:1168	phylogenetic, phenotypic and chemotaxonomic analyses	1117:1168	phylogenetic, phenotypic and chemotaxonomic analyses	1117:1168	Combined data of phylogenetic, phenotypic and chemotaxonomic analyses demonstrated that strain S20-100T represents a novel species of the genus Marmoricola, for which the name Marmoricola silvestris sp.
29498616	9	61	theme	genus	1238:1242	arg1	Marmoricola					1244:1254	the genus Marmoricola	1234:1254	the genus Marmoricola	1234:1254	Combined data of phylogenetic, phenotypic and chemotaxonomic analyses demonstrated that strain S20-100T represents a novel species of the genus Marmoricola, for which the name Marmoricola silvestris sp.
29498616	6	62	theme	lower	958:962	arg1	amounts					964:970	lower amounts	958:970	lower amounts	958:970	The strain contained MK-8(H4) as the predominant isoprenoid quinone and diphosphatidylglycerol, phosphatidylglycerol, four unidentified phospholipids and three unidentified lipids in lower amounts.
29498616	3	63	theme	0-30 °C	290:296	arg1	range					281:285	a temperature range	267:285	a temperature range of 0-30 °C	267:296	Growth occurred at a temperature range of 0-30 °C, at pH 6-9 and in the presence of 0-1 % (w/v) NaCl.
29498616	4	64	theme	rRNA	385:388	arg1	sequence					395:402	16S rRNA gene sequence	381:402	16S rRNA gene sequence	381:402	Phylogenetic analysis based on 16S rRNA gene sequence showed that strain S20-100T was related to the genus Marmoricola and had the highest 16S rRNA gene sequence similarity to Marmoricola ginsengisoli Gsoil 097T (98.4 %) and Marmoricola solisilvae KIS18-7T (98.3 %).
29498616	9	65	theme	novel	1217:1221	arg1	species					1223:1229	a novel species	1215:1229	a novel species	1215:1229	Combined data of phylogenetic, phenotypic and chemotaxonomic analyses demonstrated that strain S20-100T represents a novel species of the genus Marmoricola, for which the name Marmoricola silvestris sp.
29498616	11	66	theme	=DSM	1350:1353	arg1	30008T					1367:1372	=DSM 104694T=LMG 30008T	1350:1372	=DSM 104694T=LMG 30008T	1350:1372	The type strain is S20-100T (=DSM 104694T=LMG 30008T).
29498616	11	66	theme	=DSM	1350:1353	arg1	S20-100T					1340:1347	S20-100T	1340:1347	S20-100T (=DSM 104694T=LMG 30008T)	1340:1373	The type strain is S20-100T (=DSM 104694T=LMG 30008T).
29498616	4	67	theme	Gsoil	551:555	arg1	097T					557:560	Marmoricola ginsengisoli Gsoil 097T	526:560	Marmoricola ginsengisoli Gsoil 097T (98.4 %)	526:569	Phylogenetic analysis based on 16S rRNA gene sequence showed that strain S20-100T was related to the genus Marmoricola and had the highest 16S rRNA gene sequence similarity to Marmoricola ginsengisoli Gsoil 097T (98.4 %) and Marmoricola solisilvae KIS18-7T (98.3 %).
29498616	4	67	theme	Gsoil	551:555	arg1	%					568:568	98.4 %	563:568	98.4 %	563:568	Phylogenetic analysis based on 16S rRNA gene sequence showed that strain S20-100T was related to the genus Marmoricola and had the highest 16S rRNA gene sequence similarity to Marmoricola ginsengisoli Gsoil 097T (98.4 %) and Marmoricola solisilvae KIS18-7T (98.3 %).
29498616	4	68	theme	16S	381:383	arg1	rRNA					385:388	16S rRNA	381:388	16S rRNA gene sequence	381:402	Phylogenetic analysis based on 16S rRNA gene sequence showed that strain S20-100T was related to the genus Marmoricola and had the highest 16S rRNA gene sequence similarity to Marmoricola ginsengisoli Gsoil 097T (98.4 %) and Marmoricola solisilvae KIS18-7T (98.3 %).
29498616	6	69	contain	contained	786:794	arg2	H4					801:802	H4	801:802	H4	801:802	The strain contained MK-8(H4) as the predominant isoprenoid quinone and diphosphatidylglycerol, phosphatidylglycerol, four unidentified phospholipids and three unidentified lipids in lower amounts.
29498616	6	69	contain	contained	786:794	arg2	quinone					835:841	the predominant isoprenoid quinone	808:841	the predominant isoprenoid quinone	808:841	The strain contained MK-8(H4) as the predominant isoprenoid quinone and diphosphatidylglycerol, phosphatidylglycerol, four unidentified phospholipids and three unidentified lipids in lower amounts.
29498616	6	69	contain	contained	786:794	arg2	MK-8					796:799	MK-8	796:799	MK-8(H4)	796:803	The strain contained MK-8(H4) as the predominant isoprenoid quinone and diphosphatidylglycerol, phosphatidylglycerol, four unidentified phospholipids and three unidentified lipids in lower amounts.
29498616	6	69	contain	contained	786:794	arg1	strain					779:784	The strain	775:784	The strain	775:784	The strain contained MK-8(H4) as the predominant isoprenoid quinone and diphosphatidylglycerol, phosphatidylglycerol, four unidentified phospholipids and three unidentified lipids in lower amounts.
29498616	11	70	theme	104694T=LMG	1355:1365	arg1	30008T					1367:1372	=DSM 104694T=LMG 30008T	1350:1372	=DSM 104694T=LMG 30008T	1350:1372	The type strain is S20-100T (=DSM 104694T=LMG 30008T).
29498616	11	70	theme	104694T=LMG	1355:1365	arg1	S20-100T					1340:1347	S20-100T	1340:1347	S20-100T (=DSM 104694T=LMG 30008T)	1340:1373	The type strain is S20-100T (=DSM 104694T=LMG 30008T).
29498616	9	71	theme	Marmoricola	1276:1286	arg1	sp					1299:1300	the name Marmoricola silvestris sp	1267:1300	the name Marmoricola silvestris sp	1267:1300	Combined data of phylogenetic, phenotypic and chemotaxonomic analyses demonstrated that strain S20-100T represents a novel species of the genus Marmoricola, for which the name Marmoricola silvestris sp.
29498616	4	72	contain	had	473:475	arg2	similarity					512:521	the highest 16S rRNA gene sequence similarity	477:521	the highest 16S rRNA gene sequence similarity to Marmoricola ginsengisoli Gsoil 097T (98.4 %) and Marmoricola solisilvae KIS18-7T (98.3 %)	477:614	Phylogenetic analysis based on 16S rRNA gene sequence showed that strain S20-100T was related to the genus Marmoricola and had the highest 16S rRNA gene sequence similarity to Marmoricola ginsengisoli Gsoil 097T (98.4 %) and Marmoricola solisilvae KIS18-7T (98.3 %).
29498616	4	72	contain	had	473:475	arg1	S20-100T					423:430	strain S20-100T	416:430	strain S20-100T	416:430	Phylogenetic analysis based on 16S rRNA gene sequence showed that strain S20-100T was related to the genus Marmoricola and had the highest 16S rRNA gene sequence similarity to Marmoricola ginsengisoli Gsoil 097T (98.4 %) and Marmoricola solisilvae KIS18-7T (98.3 %).
29498616	6	73	theme	predominant	812:822	arg1	phosphatidylglycerol					871:890	phosphatidylglycerol	871:890	phosphatidylglycerol	871:890	The strain contained MK-8(H4) as the predominant isoprenoid quinone and diphosphatidylglycerol, phosphatidylglycerol, four unidentified phospholipids and three unidentified lipids in lower amounts.
29498616	6	73	theme	predominant	812:822	arg1	MK-8					796:799	MK-8	796:799	MK-8(H4)	796:803	The strain contained MK-8(H4) as the predominant isoprenoid quinone and diphosphatidylglycerol, phosphatidylglycerol, four unidentified phospholipids and three unidentified lipids in lower amounts.
29498616	6	73	theme	predominant	812:822	arg1	lipids					948:953	three unidentified lipids	929:953	three unidentified lipids	929:953	The strain contained MK-8(H4) as the predominant isoprenoid quinone and diphosphatidylglycerol, phosphatidylglycerol, four unidentified phospholipids and three unidentified lipids in lower amounts.
29498616	6	73	theme	predominant	812:822	arg1	quinone					835:841	the predominant isoprenoid quinone	808:841	the predominant isoprenoid quinone	808:841	The strain contained MK-8(H4) as the predominant isoprenoid quinone and diphosphatidylglycerol, phosphatidylglycerol, four unidentified phospholipids and three unidentified lipids in lower amounts.
29498616	6	73	theme	predominant	812:822	arg1	phospholipids					911:923	four unidentified phospholipids	893:923	four unidentified phospholipids	893:923	The strain contained MK-8(H4) as the predominant isoprenoid quinone and diphosphatidylglycerol, phosphatidylglycerol, four unidentified phospholipids and three unidentified lipids in lower amounts.
29498616	1	74	theme	novel	35:39	arg1	actinobacterium					41:55	a novel actinobacterium	33:55	a novel actinobacterium	33:55	nov., a novel actinobacterium isolated from alpine forest soil.
29498616	1	74	theme	novel	35:39	arg1	nov.					27:30	nov.	27:30	nov.	27:30	nov., a novel actinobacterium isolated from alpine forest soil.
29498616	7	75	dep	iso-C16 	1017:1024	arg1	C17 					1030:1033	C17 	1030:1033	C17 	1030:1033	The major cellular fatty acids (>10 %) were iso-C16 : 0, C17 : 1ω6c and C18 : 1ω9c.
29498616	7	75	dep	iso-C16 	1017:1024	arg1	 0					1026:1027	 0	1026:1027	 0	1026:1027	The major cellular fatty acids (>10 %) were iso-C16 : 0, C17 : 1ω6c and C18 : 1ω9c.
29498616	6	76	theme	unidentified	935:946	arg1	lipids					948:953	three unidentified lipids	929:953	three unidentified lipids	929:953	The strain contained MK-8(H4) as the predominant isoprenoid quinone and diphosphatidylglycerol, phosphatidylglycerol, four unidentified phospholipids and three unidentified lipids in lower amounts.
29498616	6	76	theme	unidentified	935:946	arg1	quinone					835:841	the predominant isoprenoid quinone	808:841	the predominant isoprenoid quinone	808:841	The strain contained MK-8(H4) as the predominant isoprenoid quinone and diphosphatidylglycerol, phosphatidylglycerol, four unidentified phospholipids and three unidentified lipids in lower amounts.
31434994	3	0	theme	Amycolatopsis	318:330	arg1	cappadoca					332:340	Amycolatopsis cappadoca	318:340	Amycolatopsis cappadoca (95.7%)	318:348	The 16S rRNA gene sequence result showed that strain SYSUP0005T shared highest sequence similarity with the type strain of Amycolatopsis cappadoca (95.7%), Amycolatopsis taiwanensis (95.4%), Amycolatopsis pigmentata (95.4%), Amycolatopsis ruanii (95.1%), and Amycolatopsis helveola (94.8%).
31434994	3	0	theme	Amycolatopsis	318:330	arg1	%					347:347	95.7%	343:347	95.7%	343:347	The 16S rRNA gene sequence result showed that strain SYSUP0005T shared highest sequence similarity with the type strain of Amycolatopsis cappadoca (95.7%), Amycolatopsis taiwanensis (95.4%), Amycolatopsis pigmentata (95.4%), Amycolatopsis ruanii (95.1%), and Amycolatopsis helveola (94.8%).
31434994	5	1	theme	Strain	612:617	arg1	SYSUP0005T					619:628	Strain SYSUP0005T	612:628	Strain SYSUP0005T	612:628	Strain SYSUP0005T had meso-diaminopimelic acid in its peptidoglycan.
31434994	9	2	theme	69.6 mol	1083:1090	arg1	content					1071:1077	The genomic DNA G + C content	1049:1077	The genomic DNA G + C content	1049:1077	The genomic DNA G + C content was 69.6 mol%.
31434994	9	2	theme	69.6 mol	1083:1090	arg1	%					1091:1091	69.6 mol%	1083:1091	69.6 mol%	1083:1091	The genomic DNA G + C content was 69.6 mol%.
31434994	4	3	theme	optimum	513:519	arg1	temperature					521:531	optimum temperature	513:531	optimum temperature	513:531	Growth occurs at 14-37 °C (optimum temperature, 28 °C), at pH 6-9 (optimum, pH 8) and in the presence of up to 6% (w/v) NaCl.
31434994	4	3	theme	optimum	513:519	arg1	28 °C					534:538	28 °C	534:538	28 °C	534:538	Growth occurs at 14-37 °C (optimum temperature, 28 °C), at pH 6-9 (optimum, pH 8) and in the presence of up to 6% (w/v) NaCl.
31434994	3	4	theme	cappadoca	332:340	arg1	strain					308:313	the type strain	299:313	the type strain of Amycolatopsis cappadoca (95.7%), Amycolatopsis taiwanensis (95.4%), Amycolatopsis pigmentata (95.4%), Amycolatopsis ruanii (95.1%), and Amycolatopsis helveola (94.8%)	299:483	The 16S rRNA gene sequence result showed that strain SYSUP0005T shared highest sequence similarity with the type strain of Amycolatopsis cappadoca (95.7%), Amycolatopsis taiwanensis (95.4%), Amycolatopsis pigmentata (95.4%), Amycolatopsis ruanii (95.1%), and Amycolatopsis helveola (94.8%).
31434994	10	5	theme	Summed	1187:1192	arg1	ω6c					1221:1223	ω6c	1221:1223	ω6c	1221:1223	The major fatty acids were iso-C16:0, anteiso-C17:0, C16:0, iso-C14:0, C17:1 ω6c, C17:0, and Summed Feature 3 (C16:1 ω7c/C16:1 ω6c).
31434994	10	5	theme	Summed	1187:1192	arg1	Feature					1194:1200	Summed Feature 3	1187:1202	Summed Feature 3 (C16:1 ω7c/C16:1 ω6c)	1187:1224	The major fatty acids were iso-C16:0, anteiso-C17:0, C16:0, iso-C14:0, C17:1 ω6c, C17:0, and Summed Feature 3 (C16:1 ω7c/C16:1 ω6c).
31434994	11	6	theme	phylogenetic	1259:1270	arg1	characters					1288:1297	phylogenetic, chemotaxonomic characters	1259:1297	phylogenetic, chemotaxonomic characters	1259:1297	On the basis of the phenotypic, phylogenetic, chemotaxonomic characters, and genomic comparison, SYSUP0005T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsis alkalitolerans sp.
31434994	3	7	theme	Amycolatopsis	454:466	arg1	helveola					468:475	Amycolatopsis helveola	454:475	Amycolatopsis helveola (94.8%)	454:483	The 16S rRNA gene sequence result showed that strain SYSUP0005T shared highest sequence similarity with the type strain of Amycolatopsis cappadoca (95.7%), Amycolatopsis taiwanensis (95.4%), Amycolatopsis pigmentata (95.4%), Amycolatopsis ruanii (95.1%), and Amycolatopsis helveola (94.8%).
31434994	3	7	theme	Amycolatopsis	454:466	arg1	%					482:482	94.8%	478:482	94.8%	478:482	The 16S rRNA gene sequence result showed that strain SYSUP0005T shared highest sequence similarity with the type strain of Amycolatopsis cappadoca (95.7%), Amycolatopsis taiwanensis (95.4%), Amycolatopsis pigmentata (95.4%), Amycolatopsis ruanii (95.1%), and Amycolatopsis helveola (94.8%).
31434994	2	8	theme	nonmotile	105:113	arg1	strain					115:120	A Gram-staining positive and nonmotile strain	76:120	A Gram-staining positive and nonmotile strain designated SYSUP0005T	76:142	A Gram-staining positive and nonmotile strain designated SYSUP0005T was isolated from tubers of Gastrodia elata Blume.
31434994	3	9	theme	gene	208:211	arg1	result					222:227	The 16S rRNA gene sequence result	195:227	The 16S rRNA gene sequence result	195:227	The 16S rRNA gene sequence result showed that strain SYSUP0005T shared highest sequence similarity with the type strain of Amycolatopsis cappadoca (95.7%), Amycolatopsis taiwanensis (95.4%), Amycolatopsis pigmentata (95.4%), Amycolatopsis ruanii (95.1%), and Amycolatopsis helveola (94.8%).
31434994	11	10	theme	phenotypic	1247:1256	arg1	characters					1288:1297	phylogenetic, chemotaxonomic characters	1259:1297	phylogenetic, chemotaxonomic characters	1259:1297	On the basis of the phenotypic, phylogenetic, chemotaxonomic characters, and genomic comparison, SYSUP0005T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsis alkalitolerans sp.
31434994	1	11	theme	Gastrodia	53:61	arg1	elata					63:67	Gastrodia elata	53:67	Gastrodia elata Blume	53:73	nov., isolated from Gastrodia elata Blume.
31434994	3	12	theme	Amycolatopsis	351:363	arg1	taiwanensis					365:375	Amycolatopsis taiwanensis	351:375	Amycolatopsis taiwanensis (95.4%)	351:383	The 16S rRNA gene sequence result showed that strain SYSUP0005T shared highest sequence similarity with the type strain of Amycolatopsis cappadoca (95.7%), Amycolatopsis taiwanensis (95.4%), Amycolatopsis pigmentata (95.4%), Amycolatopsis ruanii (95.1%), and Amycolatopsis helveola (94.8%).
31434994	3	12	theme	Amycolatopsis	351:363	arg1	%					382:382	95.4%	378:382	95.4%	378:382	The 16S rRNA gene sequence result showed that strain SYSUP0005T shared highest sequence similarity with the type strain of Amycolatopsis cappadoca (95.7%), Amycolatopsis taiwanensis (95.4%), Amycolatopsis pigmentata (95.4%), Amycolatopsis ruanii (95.1%), and Amycolatopsis helveola (94.8%).
31434994	4	13	dep	14-37 °C	503:510	arg1	temperature					521:531	optimum temperature	513:531	optimum temperature	513:531	Growth occurs at 14-37 °C (optimum temperature, 28 °C), at pH 6-9 (optimum, pH 8) and in the presence of up to 6% (w/v) NaCl.
31434994	4	13	dep	14-37 °C	503:510	arg1	28 °C					534:538	28 °C	534:538	28 °C	534:538	Growth occurs at 14-37 °C (optimum temperature, 28 °C), at pH 6-9 (optimum, pH 8) and in the presence of up to 6% (w/v) NaCl.
31434994	3	14	theme	sequence	213:220	arg1	result					222:227	The 16S rRNA gene sequence result	195:227	The 16S rRNA gene sequence result	195:227	The 16S rRNA gene sequence result showed that strain SYSUP0005T shared highest sequence similarity with the type strain of Amycolatopsis cappadoca (95.7%), Amycolatopsis taiwanensis (95.4%), Amycolatopsis pigmentata (95.4%), Amycolatopsis ruanii (95.1%), and Amycolatopsis helveola (94.8%).
31434994	7	15	theme	minor	784:788	arg1	menaquinones					790:801	minor menaquinones	784:801	minor menaquinones	784:801	The predominant menaquinone was MK-9(H4) and minor menaquinones were MK-9(H2) and MK-9(H8).
31434994	1	16	theme	elata	63:67	arg1	Blume					69:73	Gastrodia elata Blume	53:73	Gastrodia elata Blume	53:73	nov., isolated from Gastrodia elata Blume.
31434994	4	17	dep	%	598:598	arg1	w/v					601:603	w/v	601:603	w/v	601:603	Growth occurs at 14-37 °C (optimum temperature, 28 °C), at pH 6-9 (optimum, pH 8) and in the presence of up to 6% (w/v) NaCl.
31434994	4	17	dep	%	598:598	arg1	6					597:597	6	597:597	6	597:597	Growth occurs at 14-37 °C (optimum temperature, 28 °C), at pH 6-9 (optimum, pH 8) and in the presence of up to 6% (w/v) NaCl.
31434994	11	18	theme	Amycolatopsis	1375:1387	arg1	species					1354:1360	a novel species	1346:1360	a novel species	1346:1360	On the basis of the phenotypic, phylogenetic, chemotaxonomic characters, and genomic comparison, SYSUP0005T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsis alkalitolerans sp.
31434994	11	19	dep	characters	1288:1297	arg1	basis					1234:1238	basis	1234:1238	basis	1234:1238	On the basis of the phenotypic, phylogenetic, chemotaxonomic characters, and genomic comparison, SYSUP0005T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsis alkalitolerans sp.
31434994	11	19	dep	characters	1288:1297	arg1	the					1230:1232	the	1230:1232	the	1230:1232	On the basis of the phenotypic, phylogenetic, chemotaxonomic characters, and genomic comparison, SYSUP0005T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsis alkalitolerans sp.
31434994	11	19	dep	characters	1288:1297	arg1	the					1243:1245	the	1243:1245	the	1243:1245	On the basis of the phenotypic, phylogenetic, chemotaxonomic characters, and genomic comparison, SYSUP0005T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsis alkalitolerans sp.
31434994	11	20	dep	phylogenetic	1259:1270	arg1	chemotaxonomic					1273:1286	chemotaxonomic	1273:1286	chemotaxonomic	1273:1286	On the basis of the phenotypic, phylogenetic, chemotaxonomic characters, and genomic comparison, SYSUP0005T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsis alkalitolerans sp.
31434994	5	21	theme	meso-diaminopimelic	634:652	arg1	acid					654:657	meso-diaminopimelic acid	634:657	meso-diaminopimelic acid	634:657	Strain SYSUP0005T had meso-diaminopimelic acid in its peptidoglycan.
31434994	3	22	theme	16S	199:201	arg1	result					222:227	The 16S rRNA gene sequence result	195:227	The 16S rRNA gene sequence result	195:227	The 16S rRNA gene sequence result showed that strain SYSUP0005T shared highest sequence similarity with the type strain of Amycolatopsis cappadoca (95.7%), Amycolatopsis taiwanensis (95.4%), Amycolatopsis pigmentata (95.4%), Amycolatopsis ruanii (95.1%), and Amycolatopsis helveola (94.8%).
31434994	8	23	theme	unidentified	1017:1028	arg1	PL					1044:1045	PL	1044:1045	PL	1044:1045	The polar lipids were diphosphatidylglycerol (DPG); phosphatidylmonomethylethanolamine (PME), phosphatidylethanolamine (PE), phosphatidylinositol (PI), unidentified glycolipid (GL), and unidentified phospholipid (PL).
31434994	8	23	theme	unidentified	1017:1028	arg1	phospholipid					1030:1041	unidentified phospholipid	1017:1041	unidentified phospholipid (PL)	1017:1046	The polar lipids were diphosphatidylglycerol (DPG); phosphatidylmonomethylethanolamine (PME), phosphatidylethanolamine (PE), phosphatidylinositol (PI), unidentified glycolipid (GL), and unidentified phospholipid (PL).
31434994	8	24	theme	unidentified	983:994	arg1	glycolipid					996:1005	unidentified glycolipid	983:1005	unidentified glycolipid (GL)	983:1010	The polar lipids were diphosphatidylglycerol (DPG); phosphatidylmonomethylethanolamine (PME), phosphatidylethanolamine (PE), phosphatidylinositol (PI), unidentified glycolipid (GL), and unidentified phospholipid (PL).
31434994	8	24	theme	unidentified	983:994	arg1	GL					1008:1009	GL	1008:1009	GL	1008:1009	The polar lipids were diphosphatidylglycerol (DPG); phosphatidylmonomethylethanolamine (PME), phosphatidylethanolamine (PE), phosphatidylinositol (PI), unidentified glycolipid (GL), and unidentified phospholipid (PL).
31434994	9	25	theme	genomic	1053:1059	arg1	content					1071:1077	The genomic DNA G + C content	1049:1077	The genomic DNA G + C content	1049:1077	The genomic DNA G + C content was 69.6 mol%.
31434994	9	25	theme	genomic	1053:1059	arg1	%					1091:1091	69.6 mol%	1083:1091	69.6 mol%	1083:1091	The genomic DNA G + C content was 69.6 mol%.
31434994	8	26	theme	polar	835:839	arg1	diphosphatidylglycerol					853:874	diphosphatidylglycerol	853:874	diphosphatidylglycerol (DPG)	853:880	The polar lipids were diphosphatidylglycerol (DPG); phosphatidylmonomethylethanolamine (PME), phosphatidylethanolamine (PE), phosphatidylinositol (PI), unidentified glycolipid (GL), and unidentified phospholipid (PL).
31434994	8	26	theme	polar	835:839	arg1	lipids					841:846	The polar lipids	831:846	The polar lipids	831:846	The polar lipids were diphosphatidylglycerol (DPG); phosphatidylmonomethylethanolamine (PME), phosphatidylethanolamine (PE), phosphatidylinositol (PI), unidentified glycolipid (GL), and unidentified phospholipid (PL).
31434994	3	27	theme	rRNA	203:206	arg1	result					222:227	The 16S rRNA gene sequence result	195:227	The 16S rRNA gene sequence result	195:227	The 16S rRNA gene sequence result showed that strain SYSUP0005T shared highest sequence similarity with the type strain of Amycolatopsis cappadoca (95.7%), Amycolatopsis taiwanensis (95.4%), Amycolatopsis pigmentata (95.4%), Amycolatopsis ruanii (95.1%), and Amycolatopsis helveola (94.8%).
31434994	4	28	dep	6	597:597	arg1	to					594:595	to	594:595	to	594:595	Growth occurs at 14-37 °C (optimum temperature, 28 °C), at pH 6-9 (optimum, pH 8) and in the presence of up to 6% (w/v) NaCl.
31434994	0	29	theme	Amycolatopsis	0:12	arg1	alkalitolerans					14:27	Amycolatopsis alkalitolerans	0:27	Amycolatopsis alkalitolerans	0:27	Amycolatopsis alkalitolerans sp.
31434994	3	30	theme	strain	241:246	arg1	SYSUP0005T					248:257	strain SYSUP0005T	241:257	strain SYSUP0005T	241:257	The 16S rRNA gene sequence result showed that strain SYSUP0005T shared highest sequence similarity with the type strain of Amycolatopsis cappadoca (95.7%), Amycolatopsis taiwanensis (95.4%), Amycolatopsis pigmentata (95.4%), Amycolatopsis ruanii (95.1%), and Amycolatopsis helveola (94.8%).
31434994	3	31	theme	taiwanensis	365:375	arg1	strain					308:313	the type strain	299:313	the type strain of Amycolatopsis cappadoca (95.7%), Amycolatopsis taiwanensis (95.4%), Amycolatopsis pigmentata (95.4%), Amycolatopsis ruanii (95.1%), and Amycolatopsis helveola (94.8%)	299:483	The 16S rRNA gene sequence result showed that strain SYSUP0005T shared highest sequence similarity with the type strain of Amycolatopsis cappadoca (95.7%), Amycolatopsis taiwanensis (95.4%), Amycolatopsis pigmentata (95.4%), Amycolatopsis ruanii (95.1%), and Amycolatopsis helveola (94.8%).
31434994	3	32	theme	ruanii	434:439	arg1	strain					308:313	the type strain	299:313	the type strain of Amycolatopsis cappadoca (95.7%), Amycolatopsis taiwanensis (95.4%), Amycolatopsis pigmentata (95.4%), Amycolatopsis ruanii (95.1%), and Amycolatopsis helveola (94.8%)	299:483	The 16S rRNA gene sequence result showed that strain SYSUP0005T shared highest sequence similarity with the type strain of Amycolatopsis cappadoca (95.7%), Amycolatopsis taiwanensis (95.4%), Amycolatopsis pigmentata (95.4%), Amycolatopsis ruanii (95.1%), and Amycolatopsis helveola (94.8%).
31434994	13	33	theme	=KCTC	1491:1495	arg1	SYSUP0005T					1479:1488	SYSUP0005T	1479:1488	SYSUP0005T (=KCTC 49024T = CGMCC4.7463T)	1479:1518	The type strain is SYSUP0005T (=KCTC 49024T = CGMCC4.7463T).
31434994	13	33	theme	=KCTC	1491:1495	arg1	49024T = CGMCC4.7463T					1497:1517	=KCTC 49024T = CGMCC4.7463T	1491:1517	=KCTC 49024T = CGMCC4.7463T	1491:1517	The type strain is SYSUP0005T (=KCTC 49024T = CGMCC4.7463T).
31434994	10	34	theme	fatty	1104:1108	arg1	acids					1110:1114	The major fatty acids	1094:1114	The major fatty acids	1094:1114	The major fatty acids were iso-C16:0, anteiso-C17:0, C16:0, iso-C14:0, C17:1 ω6c, C17:0, and Summed Feature 3 (C16:1 ω7c/C16:1 ω6c).
31434994	10	34	theme	fatty	1104:1108	arg1	iso-C16:0					1121:1129	iso-C16:0	1121:1129	iso-C16:0	1121:1129	The major fatty acids were iso-C16:0, anteiso-C17:0, C16:0, iso-C14:0, C17:1 ω6c, C17:0, and Summed Feature 3 (C16:1 ω7c/C16:1 ω6c).
31434994	2	35	theme	Blume	188:192	arg1	tubers					162:167	tubers	162:167	tubers of Gastrodia elata Blume	162:192	A Gram-staining positive and nonmotile strain designated SYSUP0005T was isolated from tubers of Gastrodia elata Blume.
31434994	4	36	dep	NaCl	606:609	arg1	%					598:598	%	598:598	%	598:598	Growth occurs at 14-37 °C (optimum temperature, 28 °C), at pH 6-9 (optimum, pH 8) and in the presence of up to 6% (w/v) NaCl.
31434994	3	37	theme	Amycolatopsis	386:398	arg1	%					416:416	95.4%	412:416	95.4%	412:416	The 16S rRNA gene sequence result showed that strain SYSUP0005T shared highest sequence similarity with the type strain of Amycolatopsis cappadoca (95.7%), Amycolatopsis taiwanensis (95.4%), Amycolatopsis pigmentata (95.4%), Amycolatopsis ruanii (95.1%), and Amycolatopsis helveola (94.8%).
31434994	3	37	theme	Amycolatopsis	386:398	arg1	pigmentata					400:409	Amycolatopsis pigmentata	386:409	Amycolatopsis pigmentata (95.4%)	386:417	The 16S rRNA gene sequence result showed that strain SYSUP0005T shared highest sequence similarity with the type strain of Amycolatopsis cappadoca (95.7%), Amycolatopsis taiwanensis (95.4%), Amycolatopsis pigmentata (95.4%), Amycolatopsis ruanii (95.1%), and Amycolatopsis helveola (94.8%).
31434994	3	38	theme	highest	266:272	arg1	similarity					283:292	highest sequence similarity	266:292	highest sequence similarity	266:292	The 16S rRNA gene sequence result showed that strain SYSUP0005T shared highest sequence similarity with the type strain of Amycolatopsis cappadoca (95.7%), Amycolatopsis taiwanensis (95.4%), Amycolatopsis pigmentata (95.4%), Amycolatopsis ruanii (95.1%), and Amycolatopsis helveola (94.8%).
31434994	2	39	theme	elata	182:186	arg1	Blume					188:192	Gastrodia elata Blume	172:192	Gastrodia elata Blume	172:192	A Gram-staining positive and nonmotile strain designated SYSUP0005T was isolated from tubers of Gastrodia elata Blume.
31434994	4	40	dep	optimum	553:559	arg1	pH					562:563	pH 8	562:565	pH 8	562:565	Growth occurs at 14-37 °C (optimum temperature, 28 °C), at pH 6-9 (optimum, pH 8) and in the presence of up to 6% (w/v) NaCl.
31434994	3	41	theme	pigmentata	400:409	arg1	strain					308:313	the type strain	299:313	the type strain of Amycolatopsis cappadoca (95.7%), Amycolatopsis taiwanensis (95.4%), Amycolatopsis pigmentata (95.4%), Amycolatopsis ruanii (95.1%), and Amycolatopsis helveola (94.8%)	299:483	The 16S rRNA gene sequence result showed that strain SYSUP0005T shared highest sequence similarity with the type strain of Amycolatopsis cappadoca (95.7%), Amycolatopsis taiwanensis (95.4%), Amycolatopsis pigmentata (95.4%), Amycolatopsis ruanii (95.1%), and Amycolatopsis helveola (94.8%).
31434994	3	42	theme	sequence	274:281	arg1	similarity					283:292	highest sequence similarity	266:292	highest sequence similarity	266:292	The 16S rRNA gene sequence result showed that strain SYSUP0005T shared highest sequence similarity with the type strain of Amycolatopsis cappadoca (95.7%), Amycolatopsis taiwanensis (95.4%), Amycolatopsis pigmentata (95.4%), Amycolatopsis ruanii (95.1%), and Amycolatopsis helveola (94.8%).
31434994	2	43	theme	Gastrodia	172:180	arg1	elata					182:186	Gastrodia elata	172:186	Gastrodia elata Blume	172:192	A Gram-staining positive and nonmotile strain designated SYSUP0005T was isolated from tubers of Gastrodia elata Blume.
31434994	3	44	theme	Amycolatopsis	420:432	arg1	ruanii					434:439	Amycolatopsis ruanii	420:439	Amycolatopsis ruanii (95.1%)	420:447	The 16S rRNA gene sequence result showed that strain SYSUP0005T shared highest sequence similarity with the type strain of Amycolatopsis cappadoca (95.7%), Amycolatopsis taiwanensis (95.4%), Amycolatopsis pigmentata (95.4%), Amycolatopsis ruanii (95.1%), and Amycolatopsis helveola (94.8%).
31434994	3	44	theme	Amycolatopsis	420:432	arg1	%					446:446	95.1%	442:446	95.1%	442:446	The 16S rRNA gene sequence result showed that strain SYSUP0005T shared highest sequence similarity with the type strain of Amycolatopsis cappadoca (95.7%), Amycolatopsis taiwanensis (95.4%), Amycolatopsis pigmentata (95.4%), Amycolatopsis ruanii (95.1%), and Amycolatopsis helveola (94.8%).
31434994	13	45	theme	type	1464:1467	arg1	SYSUP0005T					1479:1488	SYSUP0005T	1479:1488	SYSUP0005T (=KCTC 49024T = CGMCC4.7463T)	1479:1518	The type strain is SYSUP0005T (=KCTC 49024T = CGMCC4.7463T).
31434994	13	45	theme	type	1464:1467	arg1	strain					1469:1474	The type strain	1460:1474	The type strain	1460:1474	The type strain is SYSUP0005T (=KCTC 49024T = CGMCC4.7463T).
31434994	2	46	theme	positive	92:99	arg1	strain					115:120	A Gram-staining positive and nonmotile strain	76:120	A Gram-staining positive and nonmotile strain designated SYSUP0005T	76:142	A Gram-staining positive and nonmotile strain designated SYSUP0005T was isolated from tubers of Gastrodia elata Blume.
31434994	4	47	theme	NaCl	606:609	arg1	presence					579:586	the presence	575:586	the presence of up to 6% (w/v) NaCl	575:609	Growth occurs at 14-37 °C (optimum temperature, 28 °C), at pH 6-9 (optimum, pH 8) and in the presence of up to 6% (w/v) NaCl.
31434994	2	48	theme	Gram-staining	78:90	arg1	strain					115:120	A Gram-staining positive and nonmotile strain	76:120	A Gram-staining positive and nonmotile strain designated SYSUP0005T	76:142	A Gram-staining positive and nonmotile strain designated SYSUP0005T was isolated from tubers of Gastrodia elata Blume.
31434994	11	49	theme	genus	1369:1373	arg1	Amycolatopsis					1375:1387	the genus Amycolatopsis	1365:1387	the genus Amycolatopsis	1365:1387	On the basis of the phenotypic, phylogenetic, chemotaxonomic characters, and genomic comparison, SYSUP0005T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsis alkalitolerans sp.
31434994	9	50	theme	G + C	1065:1069	arg1	content					1071:1077	The genomic DNA G + C content	1049:1077	The genomic DNA G + C content	1049:1077	The genomic DNA G + C content was 69.6 mol%.
31434994	9	50	theme	G + C	1065:1069	arg1	%					1091:1091	69.6 mol%	1083:1091	69.6 mol%	1083:1091	The genomic DNA G + C content was 69.6 mol%.
31434994	11	51	theme	name	1404:1407	arg1	alkalitolerans					1423:1436	the name Amycolatopsis alkalitolerans	1400:1436	the name Amycolatopsis alkalitolerans	1400:1436	On the basis of the phenotypic, phylogenetic, chemotaxonomic characters, and genomic comparison, SYSUP0005T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsis alkalitolerans sp.
31434994	10	52	theme	major	1098:1102	arg1	acids					1110:1114	The major fatty acids	1094:1114	The major fatty acids	1094:1114	The major fatty acids were iso-C16:0, anteiso-C17:0, C16:0, iso-C14:0, C17:1 ω6c, C17:0, and Summed Feature 3 (C16:1 ω7c/C16:1 ω6c).
31434994	10	52	theme	major	1098:1102	arg1	iso-C16:0					1121:1129	iso-C16:0	1121:1129	iso-C16:0	1121:1129	The major fatty acids were iso-C16:0, anteiso-C17:0, C16:0, iso-C14:0, C17:1 ω6c, C17:0, and Summed Feature 3 (C16:1 ω7c/C16:1 ω6c).
31434994	11	53	theme	genomic	1304:1310	arg1	comparison					1312:1321	genomic comparison	1304:1321	genomic comparison	1304:1321	On the basis of the phenotypic, phylogenetic, chemotaxonomic characters, and genomic comparison, SYSUP0005T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsis alkalitolerans sp.
31434994	3	54	theme	type	303:306	arg1	strain					308:313	the type strain	299:313	the type strain of Amycolatopsis cappadoca (95.7%), Amycolatopsis taiwanensis (95.4%), Amycolatopsis pigmentata (95.4%), Amycolatopsis ruanii (95.1%), and Amycolatopsis helveola (94.8%)	299:483	The 16S rRNA gene sequence result showed that strain SYSUP0005T shared highest sequence similarity with the type strain of Amycolatopsis cappadoca (95.7%), Amycolatopsis taiwanensis (95.4%), Amycolatopsis pigmentata (95.4%), Amycolatopsis ruanii (95.1%), and Amycolatopsis helveola (94.8%).
31434994	6	55	theme	cell	691:694	arg1	sugars					696:701	The whole cell sugars	681:701	The whole cell sugars	681:701	The whole cell sugars were galactose, ribose, and xylose.
31434994	6	55	theme	cell	691:694	arg1	galactose					708:716	galactose	708:716	galactose	708:716	The whole cell sugars were galactose, ribose, and xylose.
31434994	0	56	dep	sp	29:30	arg1	alkalitolerans					14:27	Amycolatopsis alkalitolerans	0:27	Amycolatopsis alkalitolerans	0:27	Amycolatopsis alkalitolerans sp.
31434994	11	57	theme	novel	1348:1352	arg1	species					1354:1360	a novel species	1346:1360	a novel species	1346:1360	On the basis of the phenotypic, phylogenetic, chemotaxonomic characters, and genomic comparison, SYSUP0005T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsis alkalitolerans sp.
31434994	2	58	attach	isolated	148:155	arg2	strain					115:120	A Gram-staining positive and nonmotile strain	76:120	A Gram-staining positive and nonmotile strain designated SYSUP0005T	76:142	A Gram-staining positive and nonmotile strain designated SYSUP0005T was isolated from tubers of Gastrodia elata Blume.
31434994	2	58	attach	isolated	148:155	arg1	tubers					162:167	tubers	162:167	tubers of Gastrodia elata Blume	162:192	A Gram-staining positive and nonmotile strain designated SYSUP0005T was isolated from tubers of Gastrodia elata Blume.
31434994	7	59	theme	predominant	743:753	arg1	menaquinone					755:765	The predominant menaquinone	739:765	The predominant menaquinone	739:765	The predominant menaquinone was MK-9(H4) and minor menaquinones were MK-9(H2) and MK-9(H8).
31434994	7	59	theme	predominant	743:753	arg1	MK-9					771:774	MK-9	771:774	MK-9(H4)	771:778	The predominant menaquinone was MK-9(H4) and minor menaquinones were MK-9(H2) and MK-9(H8).
31434994	5	60	contain	had	630:632	arg2	acid					654:657	meso-diaminopimelic acid	634:657	meso-diaminopimelic acid	634:657	Strain SYSUP0005T had meso-diaminopimelic acid in its peptidoglycan.
31434994	5	60	contain	had	630:632	arg1	SYSUP0005T					619:628	Strain SYSUP0005T	612:628	Strain SYSUP0005T	612:628	Strain SYSUP0005T had meso-diaminopimelic acid in its peptidoglycan.
31434994	3	61	theme	helveola	468:475	arg1	strain					308:313	the type strain	299:313	the type strain of Amycolatopsis cappadoca (95.7%), Amycolatopsis taiwanensis (95.4%), Amycolatopsis pigmentata (95.4%), Amycolatopsis ruanii (95.1%), and Amycolatopsis helveola (94.8%)	299:483	The 16S rRNA gene sequence result showed that strain SYSUP0005T shared highest sequence similarity with the type strain of Amycolatopsis cappadoca (95.7%), Amycolatopsis taiwanensis (95.4%), Amycolatopsis pigmentata (95.4%), Amycolatopsis ruanii (95.1%), and Amycolatopsis helveola (94.8%).
31434994	6	62	theme	whole	685:689	arg1	sugars					696:701	The whole cell sugars	681:701	The whole cell sugars	681:701	The whole cell sugars were galactose, ribose, and xylose.
31434994	6	62	theme	whole	685:689	arg1	galactose					708:716	galactose	708:716	galactose	708:716	The whole cell sugars were galactose, ribose, and xylose.
31434994	9	63	theme	DNA	1061:1063	arg1	content					1071:1077	The genomic DNA G + C content	1049:1077	The genomic DNA G + C content	1049:1077	The genomic DNA G + C content was 69.6 mol%.
31434994	9	63	theme	DNA	1061:1063	arg1	%					1091:1091	69.6 mol%	1083:1091	69.6 mol%	1083:1091	The genomic DNA G + C content was 69.6 mol%.
31434994	11	64	theme	Amycolatopsis	1409:1421	arg1	alkalitolerans					1423:1436	the name Amycolatopsis alkalitolerans	1400:1436	the name Amycolatopsis alkalitolerans	1400:1436	On the basis of the phenotypic, phylogenetic, chemotaxonomic characters, and genomic comparison, SYSUP0005T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsis alkalitolerans sp.
31434994	11	65	dep	sp	1438:1439	arg1	alkalitolerans					1423:1436	the name Amycolatopsis alkalitolerans	1400:1436	the name Amycolatopsis alkalitolerans	1400:1436	On the basis of the phenotypic, phylogenetic, chemotaxonomic characters, and genomic comparison, SYSUP0005T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsis alkalitolerans sp.
31248418	5	0	theme	RESULTS	835:841	arg1	progress					851:858	RESULTS Disease progress	835:858	RESULTS Disease progress	835:858	RESULTS Disease progress can be halted and blocked at the molecular level.
31248418	4	1	contain	has	770:772	arg1	treatment					760:768	this treatment	755:768	this treatment	755:768	METHODS Here, we used an NPC1 mouse model to study a splenoprotective effect of a treatment with miglustat, 2-hydroxypropyl-ß-cyclodextrin and allopregnanolone and showed that this treatment has a positive effect on spleen morphology and lipid metabolism.
31248418	4	1	contain	has	770:772	arg2	effect					785:790	a positive effect	774:790	a positive effect	774:790	METHODS Here, we used an NPC1 mouse model to study a splenoprotective effect of a treatment with miglustat, 2-hydroxypropyl-ß-cyclodextrin and allopregnanolone and showed that this treatment has a positive effect on spleen morphology and lipid metabolism.
31248418	5	2	theme	molecular	893:901	arg1	level					903:907	the molecular level	889:907	the molecular level	889:907	RESULTS Disease progress can be halted and blocked at the molecular level.
31248418	9	3	theme	subtypes	1451:1458	arg1	composition					1429:1439	the composition	1425:1439	the composition of T cell subtypes	1425:1458	CONCLUSIONS In summary, the treatment promotes normal spleen morphology, stabilization of lipid homeostasis and blocking of inflammation, but alters the composition of T cell subtypes.
31248418	0	4	theme	cholesterol	95:105	arg1	homeostasis					107:117	splenic cholesterol homeostasis	87:117	splenic cholesterol homeostasis	87:117	A therapy with miglustat, 2-hydroxypropyl-ß-cyclodextrin and allopregnanolone restores splenic cholesterol homeostasis in Niemann-pick disease type C1.
31248418	3	5	from	mechanisms	457:466	arg1	disease					542:548	NPC1 disease	537:548	NPC1 disease	537:548	The mechanisms of splenomegaly and alterations of lipid metabolism-related genes in NPC1 disease are still poorly understood.
31248418	7	6	from	cells	1113:1117	arg1	mice					1130:1133	Npc1-/- mice	1122:1133	Npc1-/- mice	1122:1133	Also, FACS analyses showed that the increased number of splenic myeloid cells in Npc1-/- mice was normalized by the treatment.
31248418	0	7	theme	Niemann-pick	122:133	arg1	C1					148:149	Niemann-pick disease type C1	122:149	Niemann-pick disease type C1	122:149	A therapy with miglustat, 2-hydroxypropyl-ß-cyclodextrin and allopregnanolone restores splenic cholesterol homeostasis in Niemann-pick disease type C1.
31248418	2	8	theme	neuronal	324:331	arg1	symptoms					346:353	other neuronal and visceral symptoms	318:353	other neuronal and visceral symptoms	318:353	Besides other neuronal and visceral symptoms, NPC1 patients develop spleen dysfunction, isolated spleno- or hepatosplenomegaly and infections.
31248418	8	9	theme	Npc1-/-	1176:1182	arg1	mice					1184:1187	Treated Npc1-/- mice	1168:1187	Treated Npc1-/- mice	1168:1187	Treated Npc1-/- mice showed decreased numbers of cytotoxic T cells and increased numbers of T helper cells.
31248418	1	10	theme	live	297:300	arg1	births					302:307	1/120,000 live births	287:307	1/120,000 live births	287:307	BACKGROUND Niemann-Pick disease type C1 (NPC1) is an autosomal-recessive lipid-storage disorder with an estimated minimal incidence of 1/120,000 live births.
31248418	6	11	theme	spleen	954:959	arg1	weight					961:966	spleen weight	954:966	spleen weight	954:966	Mutant Npc1 (Npc1-/-) mice showed increased spleen weight and increased lipid accumulation that could be avoided by our treatment.
31248418	2	12	theme	other	318:322	arg1	symptoms					346:353	other neuronal and visceral symptoms	318:353	other neuronal and visceral symptoms	318:353	Besides other neuronal and visceral symptoms, NPC1 patients develop spleen dysfunction, isolated spleno- or hepatosplenomegaly and infections.
31248418	1	13	theme	Niemann-Pick	163:174	arg1	disorder					239:246	an autosomal-recessive lipid-storage disorder	202:246	an autosomal-recessive lipid-storage disorder with an estimated minimal incidence of 1/120,000 live births	202:307	BACKGROUND Niemann-Pick disease type C1 (NPC1) is an autosomal-recessive lipid-storage disorder with an estimated minimal incidence of 1/120,000 live births.
31248418	1	13	theme	Niemann-Pick	163:174	arg1	NPC1					193:196	NPC1	193:196	NPC1	193:196	BACKGROUND Niemann-Pick disease type C1 (NPC1) is an autosomal-recessive lipid-storage disorder with an estimated minimal incidence of 1/120,000 live births.
31248418	1	13	theme	Niemann-Pick	163:174	arg1	C1					189:190	BACKGROUND Niemann-Pick disease type C1	152:190	BACKGROUND Niemann-Pick disease type C1 (NPC1)	152:197	BACKGROUND Niemann-Pick disease type C1 (NPC1) is an autosomal-recessive lipid-storage disorder with an estimated minimal incidence of 1/120,000 live births.
31248418	0	14	theme	type	143:146	arg1	C1					148:149	Niemann-pick disease type C1	122:149	Niemann-pick disease type C1	122:149	A therapy with miglustat, 2-hydroxypropyl-ß-cyclodextrin and allopregnanolone restores splenic cholesterol homeostasis in Niemann-pick disease type C1.
31248418	4	15	theme	positive	776:783	arg1	effect					785:790	a positive effect	774:790	a positive effect	774:790	METHODS Here, we used an NPC1 mouse model to study a splenoprotective effect of a treatment with miglustat, 2-hydroxypropyl-ß-cyclodextrin and allopregnanolone and showed that this treatment has a positive effect on spleen morphology and lipid metabolism.
31248418	7	16	from	mice	1130:1133	arg1	number					1087:1092	the increased number	1073:1092	the increased number of splenic myeloid cells in Npc1-/- mice	1073:1133	Also, FACS analyses showed that the increased number of splenic myeloid cells in Npc1-/- mice was normalized by the treatment.
31248418	3	17	theme	alterations	488:498	arg1	mechanisms					457:466	The mechanisms	453:466	The mechanisms of splenomegaly and alterations of lipid metabolism-related genes in NPC1 disease	453:548	The mechanisms of splenomegaly and alterations of lipid metabolism-related genes in NPC1 disease are still poorly understood.
31248418	0	18	theme	disease	135:141	arg1	C1					148:149	Niemann-pick disease type C1	122:149	Niemann-pick disease type C1	122:149	A therapy with miglustat, 2-hydroxypropyl-ß-cyclodextrin and allopregnanolone restores splenic cholesterol homeostasis in Niemann-pick disease type C1.
31248418	9	19	theme	lipid	1366:1370	arg1	homeostasis					1372:1382	lipid homeostasis	1366:1382	lipid homeostasis	1366:1382	CONCLUSIONS In summary, the treatment promotes normal spleen morphology, stabilization of lipid homeostasis and blocking of inflammation, but alters the composition of T cell subtypes.
31248418	4	20	used	used	596:599	arg2	we					593:594	we	593:594	we	593:594	METHODS Here, we used an NPC1 mouse model to study a splenoprotective effect of a treatment with miglustat, 2-hydroxypropyl-ß-cyclodextrin and allopregnanolone and showed that this treatment has a positive effect on spleen morphology and lipid metabolism.
31248418	7	21	from	number	1087:1092	arg1	mice					1130:1133	Npc1-/- mice	1122:1133	Npc1-/- mice	1122:1133	Also, FACS analyses showed that the increased number of splenic myeloid cells in Npc1-/- mice was normalized by the treatment.
31248418	8	22	theme	T	1227:1227	arg1	cells					1229:1233	cytotoxic T cells	1217:1233	cytotoxic T cells	1217:1233	Treated Npc1-/- mice showed decreased numbers of cytotoxic T cells and increased numbers of T helper cells.
31248418	5	23	theme	Disease	843:849	arg1	progress					851:858	RESULTS Disease progress	835:858	RESULTS Disease progress	835:858	RESULTS Disease progress can be halted and blocked at the molecular level.
31248418	3	24	from	disease	542:548	arg1	mechanisms					457:466	The mechanisms	453:466	The mechanisms of splenomegaly and alterations of lipid metabolism-related genes in NPC1 disease	453:548	The mechanisms of splenomegaly and alterations of lipid metabolism-related genes in NPC1 disease are still poorly understood.
31248418	7	25	theme	splenic	1097:1103	arg1	cells					1113:1117	splenic myeloid cells	1097:1117	splenic myeloid cells in Npc1-/- mice	1097:1133	Also, FACS analyses showed that the increased number of splenic myeloid cells in Npc1-/- mice was normalized by the treatment.
31248418	3	26	theme	splenomegaly	471:482	arg1	mechanisms					457:466	The mechanisms	453:466	The mechanisms of splenomegaly and alterations of lipid metabolism-related genes in NPC1 disease	453:548	The mechanisms of splenomegaly and alterations of lipid metabolism-related genes in NPC1 disease are still poorly understood.
31248418	8	27	theme	cells	1229:1233	arg1	numbers					1249:1255	increased numbers	1239:1255	increased numbers of T helper cells	1239:1273	Treated Npc1-/- mice showed decreased numbers of cytotoxic T cells and increased numbers of T helper cells.
31248418	8	27	theme	cells	1229:1233	arg1	numbers					1206:1212	decreased numbers	1196:1212	decreased numbers of cytotoxic T cells	1196:1233	Treated Npc1-/- mice showed decreased numbers of cytotoxic T cells and increased numbers of T helper cells.
31248418	4	28	theme	NPC1	604:607	arg1	model					615:619	an NPC1 mouse model	601:619	an NPC1 mouse model	601:619	METHODS Here, we used an NPC1 mouse model to study a splenoprotective effect of a treatment with miglustat, 2-hydroxypropyl-ß-cyclodextrin and allopregnanolone and showed that this treatment has a positive effect on spleen morphology and lipid metabolism.
31248418	4	29	theme	spleen	795:800	arg1	morphology					802:811	spleen morphology	795:811	spleen morphology	795:811	METHODS Here, we used an NPC1 mouse model to study a splenoprotective effect of a treatment with miglustat, 2-hydroxypropyl-ß-cyclodextrin and allopregnanolone and showed that this treatment has a positive effect on spleen morphology and lipid metabolism.
31248418	8	30	theme	decreased	1196:1204	arg1	numbers					1206:1212	decreased numbers	1196:1212	decreased numbers of cytotoxic T cells	1196:1233	Treated Npc1-/- mice showed decreased numbers of cytotoxic T cells and increased numbers of T helper cells.
31248418	8	31	theme	cytotoxic	1217:1225	arg1	cells					1229:1233	cytotoxic T cells	1217:1233	cytotoxic T cells	1217:1233	Treated Npc1-/- mice showed decreased numbers of cytotoxic T cells and increased numbers of T helper cells.
31248418	1	32	with	disorder	239:246	arg1	incidence					274:282	an estimated minimal incidence	253:282	an estimated minimal incidence of 1/120,000 live births	253:307	BACKGROUND Niemann-Pick disease type C1 (NPC1) is an autosomal-recessive lipid-storage disorder with an estimated minimal incidence of 1/120,000 live births.
31248418	2	33	theme	visceral	337:344	arg1	symptoms					346:353	other neuronal and visceral symptoms	318:353	other neuronal and visceral symptoms	318:353	Besides other neuronal and visceral symptoms, NPC1 patients develop spleen dysfunction, isolated spleno- or hepatosplenomegaly and infections.
31248418	1	34	theme	autosomal-recessive	205:223	arg1	C1					189:190	BACKGROUND Niemann-Pick disease type C1	152:190	BACKGROUND Niemann-Pick disease type C1 (NPC1)	152:197	BACKGROUND Niemann-Pick disease type C1 (NPC1) is an autosomal-recessive lipid-storage disorder with an estimated minimal incidence of 1/120,000 live births.
31248418	1	34	theme	autosomal-recessive	205:223	arg1	disorder					239:246	an autosomal-recessive lipid-storage disorder	202:246	an autosomal-recessive lipid-storage disorder with an estimated minimal incidence of 1/120,000 live births	202:307	BACKGROUND Niemann-Pick disease type C1 (NPC1) is an autosomal-recessive lipid-storage disorder with an estimated minimal incidence of 1/120,000 live births.
31248418	7	35	theme	FACS	1047:1050	arg1	analyses					1052:1059	FACS analyses	1047:1059	FACS analyses	1047:1059	Also, FACS analyses showed that the increased number of splenic myeloid cells in Npc1-/- mice was normalized by the treatment.
31248418	7	36	theme	Npc1-/-	1122:1128	arg1	mice					1130:1133	Npc1-/- mice	1122:1133	Npc1-/- mice	1122:1133	Also, FACS analyses showed that the increased number of splenic myeloid cells in Npc1-/- mice was normalized by the treatment.
31248418	6	37	theme	increased	972:980	arg1	accumulation					988:999	increased lipid accumulation	972:999	increased lipid accumulation that could be avoided by our treatment	972:1038	Mutant Npc1 (Npc1-/-) mice showed increased spleen weight and increased lipid accumulation that could be avoided by our treatment.
31248418	1	38	theme	lipid-storage	225:237	arg1	C1					189:190	BACKGROUND Niemann-Pick disease type C1	152:190	BACKGROUND Niemann-Pick disease type C1 (NPC1)	152:197	BACKGROUND Niemann-Pick disease type C1 (NPC1) is an autosomal-recessive lipid-storage disorder with an estimated minimal incidence of 1/120,000 live births.
31248418	1	38	theme	lipid-storage	225:237	arg1	disorder					239:246	an autosomal-recessive lipid-storage disorder	202:246	an autosomal-recessive lipid-storage disorder with an estimated minimal incidence of 1/120,000 live births	202:307	BACKGROUND Niemann-Pick disease type C1 (NPC1) is an autosomal-recessive lipid-storage disorder with an estimated minimal incidence of 1/120,000 live births.
31248418	8	39	theme	T	1260:1260	arg1	cells					1269:1273	T helper cells	1260:1273	T helper cells	1260:1273	Treated Npc1-/- mice showed decreased numbers of cytotoxic T cells and increased numbers of T helper cells.
31248418	4	40	theme	mouse	609:613	arg1	model					615:619	an NPC1 mouse model	601:619	an NPC1 mouse model	601:619	METHODS Here, we used an NPC1 mouse model to study a splenoprotective effect of a treatment with miglustat, 2-hydroxypropyl-ß-cyclodextrin and allopregnanolone and showed that this treatment has a positive effect on spleen morphology and lipid metabolism.
31248418	7	41	theme	myeloid	1105:1111	arg1	cells					1113:1117	splenic myeloid cells	1097:1117	splenic myeloid cells in Npc1-/- mice	1097:1133	Also, FACS analyses showed that the increased number of splenic myeloid cells in Npc1-/- mice was normalized by the treatment.
31248418	9	42	dep	CONCLUSIONS	1276:1286	arg1	alters					1418:1423	alters	1418:1423	alters the composition of T cell subtypes	1418:1458	CONCLUSIONS In summary, the treatment promotes normal spleen morphology, stabilization of lipid homeostasis and blocking of inflammation, but alters the composition of T cell subtypes.
31248418	9	42	dep	CONCLUSIONS	1276:1286	arg1	promotes					1314:1321	promotes	1314:1321	promotes normal spleen morphology, stabilization of lipid homeostasis and blocking of inflammation	1314:1411	CONCLUSIONS In summary, the treatment promotes normal spleen morphology, stabilization of lipid homeostasis and blocking of inflammation, but alters the composition of T cell subtypes.
31248418	4	43	with	treatment	661:669	arg1	allopregnanolone					722:737	allopregnanolone	722:737	allopregnanolone	722:737	METHODS Here, we used an NPC1 mouse model to study a splenoprotective effect of a treatment with miglustat, 2-hydroxypropyl-ß-cyclodextrin and allopregnanolone and showed that this treatment has a positive effect on spleen morphology and lipid metabolism.
31248418	4	43	with	treatment	661:669	arg1	2-hydroxypropyl-ß-cyclodextrin					687:716	2-hydroxypropyl-ß-cyclodextrin	687:716	2-hydroxypropyl-ß-cyclodextrin	687:716	METHODS Here, we used an NPC1 mouse model to study a splenoprotective effect of a treatment with miglustat, 2-hydroxypropyl-ß-cyclodextrin and allopregnanolone and showed that this treatment has a positive effect on spleen morphology and lipid metabolism.
31248418	4	43	with	treatment	661:669	arg1	miglustat					676:684	miglustat	676:684	miglustat	676:684	METHODS Here, we used an NPC1 mouse model to study a splenoprotective effect of a treatment with miglustat, 2-hydroxypropyl-ß-cyclodextrin and allopregnanolone and showed that this treatment has a positive effect on spleen morphology and lipid metabolism.
31248418	3	44	theme	lipid	503:507	arg1	genes					528:532	lipid metabolism-related genes	503:532	lipid metabolism-related genes	503:532	The mechanisms of splenomegaly and alterations of lipid metabolism-related genes in NPC1 disease are still poorly understood.
31248418	9	45	theme	T	1444:1444	arg1	subtypes					1451:1458	T cell subtypes	1444:1458	T cell subtypes	1444:1458	CONCLUSIONS In summary, the treatment promotes normal spleen morphology, stabilization of lipid homeostasis and blocking of inflammation, but alters the composition of T cell subtypes.
31248418	4	46	dep	METHODS	579:585	arg1	used					596:599	used	596:599	used an NPC1 mouse model to study a splenoprotective effect of a treatment with miglustat, 2-hydroxypropyl-ß-cyclodextrin and allopregnanolone	596:737	METHODS Here, we used an NPC1 mouse model to study a splenoprotective effect of a treatment with miglustat, 2-hydroxypropyl-ß-cyclodextrin and allopregnanolone and showed that this treatment has a positive effect on spleen morphology and lipid metabolism.
31248418	4	46	dep	METHODS	579:585	arg1	showed					743:748	showed	743:748	showed that this treatment has a positive effect on spleen morphology and lipid metabolism	743:832	METHODS Here, we used an NPC1 mouse model to study a splenoprotective effect of a treatment with miglustat, 2-hydroxypropyl-ß-cyclodextrin and allopregnanolone and showed that this treatment has a positive effect on spleen morphology and lipid metabolism.
31248418	7	47	theme	cells	1113:1117	arg1	number					1087:1092	the increased number	1073:1092	the increased number of splenic myeloid cells in Npc1-/- mice	1073:1133	Also, FACS analyses showed that the increased number of splenic myeloid cells in Npc1-/- mice was normalized by the treatment.
31248418	4	48	theme	treatment	661:669	arg1	effect					649:654	a splenoprotective effect	630:654	a splenoprotective effect of a treatment with miglustat, 2-hydroxypropyl-ß-cyclodextrin and allopregnanolone	630:737	METHODS Here, we used an NPC1 mouse model to study a splenoprotective effect of a treatment with miglustat, 2-hydroxypropyl-ß-cyclodextrin and allopregnanolone and showed that this treatment has a positive effect on spleen morphology and lipid metabolism.
31248418	9	49	theme	spleen	1330:1335	arg1	morphology					1337:1346	normal spleen morphology	1323:1346	normal spleen morphology	1323:1346	CONCLUSIONS In summary, the treatment promotes normal spleen morphology, stabilization of lipid homeostasis and blocking of inflammation, but alters the composition of T cell subtypes.
31248418	3	50	theme	metabolism-related	509:526	arg1	genes					528:532	lipid metabolism-related genes	503:532	lipid metabolism-related genes	503:532	The mechanisms of splenomegaly and alterations of lipid metabolism-related genes in NPC1 disease are still poorly understood.
31248418	8	51	theme	cells	1269:1273	arg1	numbers					1249:1255	increased numbers	1239:1255	increased numbers of T helper cells	1239:1273	Treated Npc1-/- mice showed decreased numbers of cytotoxic T cells and increased numbers of T helper cells.
31248418	8	51	theme	cells	1269:1273	arg1	numbers					1206:1212	decreased numbers	1196:1212	decreased numbers of cytotoxic T cells	1196:1233	Treated Npc1-/- mice showed decreased numbers of cytotoxic T cells and increased numbers of T helper cells.
31248418	6	52	theme	Npc1	917:920	arg1	mice					932:935	Mutant Npc1 (Npc1-/-) mice	910:935	Mutant Npc1 (Npc1-/-) mice	910:935	Mutant Npc1 (Npc1-/-) mice showed increased spleen weight and increased lipid accumulation that could be avoided by our treatment.
31248418	8	53	theme	Treated	1168:1174	arg1	mice					1184:1187	Treated Npc1-/- mice	1168:1187	Treated Npc1-/- mice	1168:1187	Treated Npc1-/- mice showed decreased numbers of cytotoxic T cells and increased numbers of T helper cells.
31248418	2	54	theme	spleen	378:383	arg1	dysfunction					385:395	spleen dysfunction	378:395	spleen dysfunction	378:395	Besides other neuronal and visceral symptoms, NPC1 patients develop spleen dysfunction, isolated spleno- or hepatosplenomegaly and infections.
31248418	8	55	theme	helper	1262:1267	arg1	cells					1269:1273	T helper cells	1260:1273	T helper cells	1260:1273	Treated Npc1-/- mice showed decreased numbers of cytotoxic T cells and increased numbers of T helper cells.
31248418	9	56	theme	normal	1323:1328	arg1	morphology					1337:1346	normal spleen morphology	1323:1346	normal spleen morphology	1323:1346	CONCLUSIONS In summary, the treatment promotes normal spleen morphology, stabilization of lipid homeostasis and blocking of inflammation, but alters the composition of T cell subtypes.
31248418	3	57	theme	NPC1	537:540	arg1	disease					542:548	NPC1 disease	537:548	NPC1 disease	537:548	The mechanisms of splenomegaly and alterations of lipid metabolism-related genes in NPC1 disease are still poorly understood.
31248418	6	58	theme	Mutant	910:915	arg1	mice					932:935	Mutant Npc1 (Npc1-/-) mice	910:935	Mutant Npc1 (Npc1-/-) mice	910:935	Mutant Npc1 (Npc1-/-) mice showed increased spleen weight and increased lipid accumulation that could be avoided by our treatment.
31248418	1	59	theme	type	184:187	arg1	disorder					239:246	an autosomal-recessive lipid-storage disorder	202:246	an autosomal-recessive lipid-storage disorder with an estimated minimal incidence of 1/120,000 live births	202:307	BACKGROUND Niemann-Pick disease type C1 (NPC1) is an autosomal-recessive lipid-storage disorder with an estimated minimal incidence of 1/120,000 live births.
31248418	1	59	theme	type	184:187	arg1	NPC1					193:196	NPC1	193:196	NPC1	193:196	BACKGROUND Niemann-Pick disease type C1 (NPC1) is an autosomal-recessive lipid-storage disorder with an estimated minimal incidence of 1/120,000 live births.
31248418	1	59	theme	type	184:187	arg1	C1					189:190	BACKGROUND Niemann-Pick disease type C1	152:190	BACKGROUND Niemann-Pick disease type C1 (NPC1)	152:197	BACKGROUND Niemann-Pick disease type C1 (NPC1) is an autosomal-recessive lipid-storage disorder with an estimated minimal incidence of 1/120,000 live births.
31248418	0	60	with	therapy	2:8	arg1	2-hydroxypropyl-ß-cyclodextrin					26:55	2-hydroxypropyl-ß-cyclodextrin	26:55	2-hydroxypropyl-ß-cyclodextrin	26:55	A therapy with miglustat, 2-hydroxypropyl-ß-cyclodextrin and allopregnanolone restores splenic cholesterol homeostasis in Niemann-pick disease type C1.
31248418	0	60	with	therapy	2:8	arg1	miglustat					15:23	miglustat	15:23	miglustat	15:23	A therapy with miglustat, 2-hydroxypropyl-ß-cyclodextrin and allopregnanolone restores splenic cholesterol homeostasis in Niemann-pick disease type C1.
31248418	0	60	with	therapy	2:8	arg1	allopregnanolone					61:76	allopregnanolone	61:76	allopregnanolone	61:76	A therapy with miglustat, 2-hydroxypropyl-ß-cyclodextrin and allopregnanolone restores splenic cholesterol homeostasis in Niemann-pick disease type C1.
31248418	7	61	theme	increased	1077:1085	arg1	number					1087:1092	the increased number	1073:1092	the increased number of splenic myeloid cells in Npc1-/- mice	1073:1133	Also, FACS analyses showed that the increased number of splenic myeloid cells in Npc1-/- mice was normalized by the treatment.
31248418	9	62	theme	inflammation	1400:1411	arg1	morphology					1337:1346	normal spleen morphology	1323:1346	normal spleen morphology	1323:1346	CONCLUSIONS In summary, the treatment promotes normal spleen morphology, stabilization of lipid homeostasis and blocking of inflammation, but alters the composition of T cell subtypes.
31248418	9	62	theme	inflammation	1400:1411	arg1	blocking					1388:1395	blocking	1388:1395	blocking of inflammation	1388:1411	CONCLUSIONS In summary, the treatment promotes normal spleen morphology, stabilization of lipid homeostasis and blocking of inflammation, but alters the composition of T cell subtypes.
31248418	9	62	theme	inflammation	1400:1411	arg1	stabilization					1349:1361	stabilization	1349:1361	stabilization of lipid homeostasis	1349:1382	CONCLUSIONS In summary, the treatment promotes normal spleen morphology, stabilization of lipid homeostasis and blocking of inflammation, but alters the composition of T cell subtypes.
31248418	1	63	theme	BACKGROUND	152:161	arg1	disorder					239:246	an autosomal-recessive lipid-storage disorder	202:246	an autosomal-recessive lipid-storage disorder with an estimated minimal incidence of 1/120,000 live births	202:307	BACKGROUND Niemann-Pick disease type C1 (NPC1) is an autosomal-recessive lipid-storage disorder with an estimated minimal incidence of 1/120,000 live births.
31248418	1	63	theme	BACKGROUND	152:161	arg1	NPC1					193:196	NPC1	193:196	NPC1	193:196	BACKGROUND Niemann-Pick disease type C1 (NPC1) is an autosomal-recessive lipid-storage disorder with an estimated minimal incidence of 1/120,000 live births.
31248418	1	63	theme	BACKGROUND	152:161	arg1	C1					189:190	BACKGROUND Niemann-Pick disease type C1	152:190	BACKGROUND Niemann-Pick disease type C1 (NPC1)	152:197	BACKGROUND Niemann-Pick disease type C1 (NPC1) is an autosomal-recessive lipid-storage disorder with an estimated minimal incidence of 1/120,000 live births.
31248418	4	64	theme	lipid	817:821	arg1	metabolism					823:832	lipid metabolism	817:832	lipid metabolism	817:832	METHODS Here, we used an NPC1 mouse model to study a splenoprotective effect of a treatment with miglustat, 2-hydroxypropyl-ß-cyclodextrin and allopregnanolone and showed that this treatment has a positive effect on spleen morphology and lipid metabolism.
31248418	6	65	dep	showed	937:942	arg1	increased					944:952	increased	944:952	showed increased spleen weight and increased lipid accumulation that could be avoided by our treatment	937:1038	Mutant Npc1 (Npc1-/-) mice showed increased spleen weight and increased lipid accumulation that could be avoided by our treatment.
31248418	3	66	from	splenomegaly	471:482	arg1	disease					542:548	NPC1 disease	537:548	NPC1 disease	537:548	The mechanisms of splenomegaly and alterations of lipid metabolism-related genes in NPC1 disease are still poorly understood.
31248418	2	67	theme	NPC1	356:359	arg1	patients					361:368	NPC1 patients	356:368	NPC1 patients	356:368	Besides other neuronal and visceral symptoms, NPC1 patients develop spleen dysfunction, isolated spleno- or hepatosplenomegaly and infections.
31248418	1	68	theme	births	302:307	arg1	incidence					274:282	an estimated minimal incidence	253:282	an estimated minimal incidence of 1/120,000 live births	253:307	BACKGROUND Niemann-Pick disease type C1 (NPC1) is an autosomal-recessive lipid-storage disorder with an estimated minimal incidence of 1/120,000 live births.
31248418	1	69	theme	estimated	256:264	arg1	incidence					274:282	an estimated minimal incidence	253:282	an estimated minimal incidence of 1/120,000 live births	253:307	BACKGROUND Niemann-Pick disease type C1 (NPC1) is an autosomal-recessive lipid-storage disorder with an estimated minimal incidence of 1/120,000 live births.
31248418	0	70	theme	splenic	87:93	arg1	homeostasis					107:117	splenic cholesterol homeostasis	87:117	splenic cholesterol homeostasis	87:117	A therapy with miglustat, 2-hydroxypropyl-ß-cyclodextrin and allopregnanolone restores splenic cholesterol homeostasis in Niemann-pick disease type C1.
31248418	9	71	theme	homeostasis	1372:1382	arg1	morphology					1337:1346	normal spleen morphology	1323:1346	normal spleen morphology	1323:1346	CONCLUSIONS In summary, the treatment promotes normal spleen morphology, stabilization of lipid homeostasis and blocking of inflammation, but alters the composition of T cell subtypes.
31248418	9	71	theme	homeostasis	1372:1382	arg1	blocking					1388:1395	blocking	1388:1395	blocking of inflammation	1388:1411	CONCLUSIONS In summary, the treatment promotes normal spleen morphology, stabilization of lipid homeostasis and blocking of inflammation, but alters the composition of T cell subtypes.
31248418	9	71	theme	homeostasis	1372:1382	arg1	stabilization					1349:1361	stabilization	1349:1361	stabilization of lipid homeostasis	1349:1382	CONCLUSIONS In summary, the treatment promotes normal spleen morphology, stabilization of lipid homeostasis and blocking of inflammation, but alters the composition of T cell subtypes.
31248418	3	72	theme	genes	528:532	arg1	alterations					488:498	alterations	488:498	alterations of lipid metabolism-related genes in NPC1 disease	488:548	The mechanisms of splenomegaly and alterations of lipid metabolism-related genes in NPC1 disease are still poorly understood.
31248418	3	72	theme	genes	528:532	arg1	splenomegaly					471:482	splenomegaly	471:482	splenomegaly	471:482	The mechanisms of splenomegaly and alterations of lipid metabolism-related genes in NPC1 disease are still poorly understood.
31248418	1	73	theme	disease	176:182	arg1	disorder					239:246	an autosomal-recessive lipid-storage disorder	202:246	an autosomal-recessive lipid-storage disorder with an estimated minimal incidence of 1/120,000 live births	202:307	BACKGROUND Niemann-Pick disease type C1 (NPC1) is an autosomal-recessive lipid-storage disorder with an estimated minimal incidence of 1/120,000 live births.
31248418	1	73	theme	disease	176:182	arg1	NPC1					193:196	NPC1	193:196	NPC1	193:196	BACKGROUND Niemann-Pick disease type C1 (NPC1) is an autosomal-recessive lipid-storage disorder with an estimated minimal incidence of 1/120,000 live births.
31248418	1	73	theme	disease	176:182	arg1	C1					189:190	BACKGROUND Niemann-Pick disease type C1	152:190	BACKGROUND Niemann-Pick disease type C1 (NPC1)	152:197	BACKGROUND Niemann-Pick disease type C1 (NPC1) is an autosomal-recessive lipid-storage disorder with an estimated minimal incidence of 1/120,000 live births.
31248418	1	74	theme	minimal	266:272	arg1	incidence					274:282	an estimated minimal incidence	253:282	an estimated minimal incidence of 1/120,000 live births	253:307	BACKGROUND Niemann-Pick disease type C1 (NPC1) is an autosomal-recessive lipid-storage disorder with an estimated minimal incidence of 1/120,000 live births.
31248418	4	75	theme	splenoprotective	632:647	arg1	effect					649:654	a splenoprotective effect	630:654	a splenoprotective effect of a treatment with miglustat, 2-hydroxypropyl-ß-cyclodextrin and allopregnanolone	630:737	METHODS Here, we used an NPC1 mouse model to study a splenoprotective effect of a treatment with miglustat, 2-hydroxypropyl-ß-cyclodextrin and allopregnanolone and showed that this treatment has a positive effect on spleen morphology and lipid metabolism.
31248418	6	76	theme	lipid	982:986	arg1	accumulation					988:999	increased lipid accumulation	972:999	increased lipid accumulation that could be avoided by our treatment	972:1038	Mutant Npc1 (Npc1-/-) mice showed increased spleen weight and increased lipid accumulation that could be avoided by our treatment.
31248418	8	77	theme	increased	1239:1247	arg1	numbers					1249:1255	increased numbers	1239:1255	increased numbers of T helper cells	1239:1273	Treated Npc1-/- mice showed decreased numbers of cytotoxic T cells and increased numbers of T helper cells.
31248418	3	78	from	alterations	488:498	arg1	disease					542:548	NPC1 disease	537:548	NPC1 disease	537:548	The mechanisms of splenomegaly and alterations of lipid metabolism-related genes in NPC1 disease are still poorly understood.
31248418	2	79	theme	isolated	398:405	arg1	spleno-					407:413	spleno-	407:413	spleno-	407:413	Besides other neuronal and visceral symptoms, NPC1 patients develop spleen dysfunction, isolated spleno- or hepatosplenomegaly and infections.
31248418	9	80	theme	cell	1446:1449	arg1	subtypes					1451:1458	T cell subtypes	1444:1458	T cell subtypes	1444:1458	CONCLUSIONS In summary, the treatment promotes normal spleen morphology, stabilization of lipid homeostasis and blocking of inflammation, but alters the composition of T cell subtypes.
31108821	9	0	theme	equivalent	1197:1206	arg1	TEAC					1230:1233	TEAC	1230:1233	TEAC	1230:1233	The soluble and insoluble phenolic fractions from monguba seeds showed high antioxidant activity measured by the oxygen radical absorption capacity (ORAC) and the trolox equivalent antioxidant capacity (TEAC) assays.
31108821	9	0	theme	equivalent	1197:1206	arg1	capacity					1220:1227	the trolox equivalent antioxidant capacity	1186:1227	the trolox equivalent antioxidant capacity (TEAC)	1186:1234	The soluble and insoluble phenolic fractions from monguba seeds showed high antioxidant activity measured by the oxygen radical absorption capacity (ORAC) and the trolox equivalent antioxidant capacity (TEAC) assays.
31108821	8	1	theme	lipid	947:951	arg1	content					953:959	high lipid content	942:959	high lipid content	942:959	Monguba seed showed high lipid content, in which the main compounds were palmitic acid and γ-tocopherol.
31108821	10	2	theme	monguba	1259:1265	arg1	seeds					1267:1271	the monguba seeds	1255:1271	the monguba seeds	1255:1271	Therefore, the monguba seeds have great potential to be explored by food, pharmaceutical and cosmetic industries due to their chemical composition.
31108821	7	3	theme	4-hydroxybenzoic	820:835	arg1	compounds					864:872	the main phenolic compounds	846:872	the main phenolic compounds	846:872	UHPLC-ESI-MS/MS analysis showed caffeic, ferulic and 4-hydroxybenzoic acids as the main phenolic compounds, mainly in the esterified form, in these seeds.
31108821	7	3	theme	4-hydroxybenzoic	820:835	arg1	acids					837:841	caffeic, ferulic and 4-hydroxybenzoic acids	799:841	caffeic, ferulic and 4-hydroxybenzoic acids	799:841	UHPLC-ESI-MS/MS analysis showed caffeic, ferulic and 4-hydroxybenzoic acids as the main phenolic compounds, mainly in the esterified form, in these seeds.
31108821	10	4	theme	due	1357:1359	arg1	industries					1346:1355	food, pharmaceutical and cosmetic industries	1312:1355	food, pharmaceutical and cosmetic industries due to their chemical composition	1312:1389	Therefore, the monguba seeds have great potential to be explored by food, pharmaceutical and cosmetic industries due to their chemical composition.
31108821	9	5	theme	antioxidant	1208:1218	arg1	TEAC					1230:1233	TEAC	1230:1233	TEAC	1230:1233	The soluble and insoluble phenolic fractions from monguba seeds showed high antioxidant activity measured by the oxygen radical absorption capacity (ORAC) and the trolox equivalent antioxidant capacity (TEAC) assays.
31108821	9	5	theme	antioxidant	1208:1218	arg1	capacity					1220:1227	the trolox equivalent antioxidant capacity	1186:1227	the trolox equivalent antioxidant capacity (TEAC)	1186:1234	The soluble and insoluble phenolic fractions from monguba seeds showed high antioxidant activity measured by the oxygen radical absorption capacity (ORAC) and the trolox equivalent antioxidant capacity (TEAC) assays.
31108821	8	6	theme	high	942:945	arg1	content					953:959	high lipid content	942:959	high lipid content	942:959	Monguba seed showed high lipid content, in which the main compounds were palmitic acid and γ-tocopherol.
31108821	7	7	theme	esterified	889:898	arg1	form					900:903	the esterified form	885:903	the esterified form	885:903	UHPLC-ESI-MS/MS analysis showed caffeic, ferulic and 4-hydroxybenzoic acids as the main phenolic compounds, mainly in the esterified form, in these seeds.
31108821	9	8	theme	radical	1147:1153	arg1	capacity					1166:1173	oxygen radical absorption capacity	1140:1173	oxygen radical absorption capacity (ORAC)	1140:1180	The soluble and insoluble phenolic fractions from monguba seeds showed high antioxidant activity measured by the oxygen radical absorption capacity (ORAC) and the trolox equivalent antioxidant capacity (TEAC) assays.
31108821	9	8	theme	radical	1147:1153	arg1	ORAC					1176:1179	ORAC	1176:1179	ORAC	1176:1179	The soluble and insoluble phenolic fractions from monguba seeds showed high antioxidant activity measured by the oxygen radical absorption capacity (ORAC) and the trolox equivalent antioxidant capacity (TEAC) assays.
31108821	9	9	theme	capacity	1220:1227	arg1	assays					1236:1241	the oxygen radical absorption capacity (ORAC) and the trolox equivalent antioxidant capacity (TEAC) assays	1136:1241	the oxygen radical absorption capacity (ORAC) and the trolox equivalent antioxidant capacity (TEAC) assays	1136:1241	The soluble and insoluble phenolic fractions from monguba seeds showed high antioxidant activity measured by the oxygen radical absorption capacity (ORAC) and the trolox equivalent antioxidant capacity (TEAC) assays.
31108821	10	10	theme	cosmetic	1337:1344	arg1	industries					1346:1355	food, pharmaceutical and cosmetic industries	1312:1355	food, pharmaceutical and cosmetic industries due to their chemical composition	1312:1389	Therefore, the monguba seeds have great potential to be explored by food, pharmaceutical and cosmetic industries due to their chemical composition.
31108821	6	11	theme	new	737:739	arg1	seeds					722:726	monguba seeds	714:726	monguba seeds	714:726	The mineral assay showed high amount of minerals, namely potassium, calcium, magnesium and zinc, which indicate that monguba seeds can be a new source of these minerals.
31108821	6	11	theme	new	737:739	arg1	source					741:746	a new source	735:746	a new source of these minerals	735:764	The mineral assay showed high amount of minerals, namely potassium, calcium, magnesium and zinc, which indicate that monguba seeds can be a new source of these minerals.
31108821	7	12	theme	ferulic	808:814	arg1	compounds					864:872	the main phenolic compounds	846:872	the main phenolic compounds	846:872	UHPLC-ESI-MS/MS analysis showed caffeic, ferulic and 4-hydroxybenzoic acids as the main phenolic compounds, mainly in the esterified form, in these seeds.
31108821	7	12	theme	ferulic	808:814	arg1	acids					837:841	caffeic, ferulic and 4-hydroxybenzoic acids	799:841	caffeic, ferulic and 4-hydroxybenzoic acids	799:841	UHPLC-ESI-MS/MS analysis showed caffeic, ferulic and 4-hydroxybenzoic acids as the main phenolic compounds, mainly in the esterified form, in these seeds.
31108821	8	13	from	γ-tocopherol	1013:1024	arg1	content					953:959	high lipid content	942:959	high lipid content	942:959	Monguba seed showed high lipid content, in which the main compounds were palmitic acid and γ-tocopherol.
31108821	10	14	theme	great	1278:1282	arg1	potential					1284:1292	great potential	1278:1292	great potential	1278:1292	Therefore, the monguba seeds have great potential to be explored by food, pharmaceutical and cosmetic industries due to their chemical composition.
31108821	5	15	theme	raffinose	554:562	arg1	oligosaccharides					571:586	the raffinose family oligosaccharides	550:586	the raffinose family oligosaccharides	550:586	These seeds showed high total content of sugars, mainly sucrose, whereas the content of the raffinose family oligosaccharides was low.
31108821	5	16	theme	oligosaccharides	571:586	arg1	low					592:594	low	592:594	low	592:594	These seeds showed high total content of sugars, mainly sucrose, whereas the content of the raffinose family oligosaccharides was low.
31108821	5	16	theme	oligosaccharides	571:586	arg1	content					539:545	the content	535:545	the content of the raffinose family oligosaccharides	535:586	These seeds showed high total content of sugars, mainly sucrose, whereas the content of the raffinose family oligosaccharides was low.
31108821	5	17	theme	family	564:569	arg1	oligosaccharides					571:586	the raffinose family oligosaccharides	550:586	the raffinose family oligosaccharides	550:586	These seeds showed high total content of sugars, mainly sucrose, whereas the content of the raffinose family oligosaccharides was low.
31108821	2	18	theme	popular	231:237	arg1	medicine					239:246	popular medicine	231:246	popular medicine	231:246	These nutritious seeds also have been used in popular medicine to treat several diseases.
31108821	7	19	theme	caffeic	799:805	arg1	compounds					864:872	the main phenolic compounds	846:872	the main phenolic compounds	846:872	UHPLC-ESI-MS/MS analysis showed caffeic, ferulic and 4-hydroxybenzoic acids as the main phenolic compounds, mainly in the esterified form, in these seeds.
31108821	7	19	theme	caffeic	799:805	arg1	acids					837:841	caffeic, ferulic and 4-hydroxybenzoic acids	799:841	caffeic, ferulic and 4-hydroxybenzoic acids	799:841	UHPLC-ESI-MS/MS analysis showed caffeic, ferulic and 4-hydroxybenzoic acids as the main phenolic compounds, mainly in the esterified form, in these seeds.
31108821	3	20	theme	functional	309:318	arg1	potential					320:328	the nutritional and functional potential	289:328	the nutritional and functional potential of monguba seed	289:344	Nevertheless, the nutritional and functional potential of monguba seed is still underexploited.
31108821	4	21	theme	seeds	455:459	arg1	components					433:442	the nutritional and functional components	402:442	the nutritional and functional components of monguba seeds	402:459	In this sense, we investigated the nutritional and functional components of monguba seeds.
31108821	10	22	theme	chemical	1370:1377	arg1	composition					1379:1389	their chemical composition	1364:1389	their chemical composition	1364:1389	Therefore, the monguba seeds have great potential to be explored by food, pharmaceutical and cosmetic industries due to their chemical composition.
31108821	10	23	theme	pharmaceutical	1318:1331	arg1	industries					1346:1355	food, pharmaceutical and cosmetic industries	1312:1355	food, pharmaceutical and cosmetic industries due to their chemical composition	1312:1389	Therefore, the monguba seeds have great potential to be explored by food, pharmaceutical and cosmetic industries due to their chemical composition.
31108821	7	24	theme	UHPLC-ESI-MS/MS	767:781	arg1	analysis					783:790	UHPLC-ESI-MS/MS analysis	767:790	UHPLC-ESI-MS/MS analysis	767:790	UHPLC-ESI-MS/MS analysis showed caffeic, ferulic and 4-hydroxybenzoic acids as the main phenolic compounds, mainly in the esterified form, in these seeds.
31108821	9	25	from	seeds	1085:1089	arg1	fractions					1062:1070	The soluble and insoluble phenolic fractions	1027:1070	The soluble and insoluble phenolic fractions from monguba seeds	1027:1089	The soluble and insoluble phenolic fractions from monguba seeds showed high antioxidant activity measured by the oxygen radical absorption capacity (ORAC) and the trolox equivalent antioxidant capacity (TEAC) assays.
31108821	0	26	theme	Composition	9:19	arg1	Pachira					58:64	Pachira	58:64	Pachira	58:64	Chemical Composition and Antioxidant Activity of Monguba (Pachira aquatica) Seeds.
31108821	0	26	theme	Composition	9:19	arg1	Seeds					76:80	Chemical Composition and Antioxidant Activity of Monguba (Pachira aquatica) Seeds	0:80	Chemical Composition and Antioxidant Activity of Monguba (Pachira aquatica) Seeds.	0:81	Chemical Composition and Antioxidant Activity of Monguba (Pachira aquatica) Seeds.
31108821	4	27	theme	monguba	447:453	arg1	seeds					455:459	monguba seeds	447:459	monguba seeds	447:459	In this sense, we investigated the nutritional and functional components of monguba seeds.
31108821	8	28	theme	main	975:978	arg1	compounds					980:988	the main compounds	971:988	the main compounds	971:988	Monguba seed showed high lipid content, in which the main compounds were palmitic acid and γ-tocopherol.
31108821	8	28	theme	main	975:978	arg1	acid					1004:1007	palmitic acid	995:1007	palmitic acid	995:1007	Monguba seed showed high lipid content, in which the main compounds were palmitic acid and γ-tocopherol.
31108821	10	29	theme	food	1312:1315	arg1	industries					1346:1355	food, pharmaceutical and cosmetic industries	1312:1355	food, pharmaceutical and cosmetic industries due to their chemical composition	1312:1389	Therefore, the monguba seeds have great potential to be explored by food, pharmaceutical and cosmetic industries due to their chemical composition.
31108821	2	30	used	used	223:226	arg2	seeds					202:206	These nutritious seeds	185:206	These nutritious seeds	185:206	These nutritious seeds also have been used in popular medicine to treat several diseases.
31108821	0	31	theme	Chemical	0:7	arg1	Composition					9:19	Chemical Composition	0:19	Chemical Composition	0:19	Chemical Composition and Antioxidant Activity of Monguba (Pachira aquatica) Seeds.
31108821	1	32	with	seed	103:106	arg1	flavor					129:134	a chestnut-like flavor	113:134	a chestnut-like flavor that can be consumed boiled	113:162	Monguba fruit has a seed with a chestnut-like flavor that can be consumed boiled, fried, and roasted.
31108821	1	32	with	seed	103:106	arg1	fried					165:169	fried	165:169	fried	165:169	Monguba fruit has a seed with a chestnut-like flavor that can be consumed boiled, fried, and roasted.
31108821	1	32	with	seed	103:106	arg1	roasted					176:182	roasted	176:182	roasted	176:182	Monguba fruit has a seed with a chestnut-like flavor that can be consumed boiled, fried, and roasted.
31108821	6	33	theme	monguba	714:720	arg1	seeds					722:726	monguba seeds	714:726	monguba seeds	714:726	The mineral assay showed high amount of minerals, namely potassium, calcium, magnesium and zinc, which indicate that monguba seeds can be a new source of these minerals.
31108821	6	33	theme	monguba	714:720	arg1	source					741:746	a new source	735:746	a new source of these minerals	735:764	The mineral assay showed high amount of minerals, namely potassium, calcium, magnesium and zinc, which indicate that monguba seeds can be a new source of these minerals.
31108821	1	34	theme	Monguba	83:89	arg1	fruit					91:95	Monguba fruit	83:95	Monguba fruit	83:95	Monguba fruit has a seed with a chestnut-like flavor that can be consumed boiled, fried, and roasted.
31108821	6	35	theme	minerals	637:644	arg1	potassium					654:662	potassium	654:662	potassium	654:662	The mineral assay showed high amount of minerals, namely potassium, calcium, magnesium and zinc, which indicate that monguba seeds can be a new source of these minerals.
31108821	6	35	theme	minerals	637:644	arg1	zinc					688:691	zinc	688:691	zinc	688:691	The mineral assay showed high amount of minerals, namely potassium, calcium, magnesium and zinc, which indicate that monguba seeds can be a new source of these minerals.
31108821	6	35	theme	minerals	637:644	arg1	amount					627:632	high amount	622:632	high amount	622:632	The mineral assay showed high amount of minerals, namely potassium, calcium, magnesium and zinc, which indicate that monguba seeds can be a new source of these minerals.
31108821	6	35	theme	minerals	637:644	arg1	calcium					665:671	calcium	665:671	calcium	665:671	The mineral assay showed high amount of minerals, namely potassium, calcium, magnesium and zinc, which indicate that monguba seeds can be a new source of these minerals.
31108821	6	35	theme	minerals	637:644	arg1	minerals					637:644	minerals	637:644	minerals	637:644	The mineral assay showed high amount of minerals, namely potassium, calcium, magnesium and zinc, which indicate that monguba seeds can be a new source of these minerals.
31108821	6	35	theme	minerals	637:644	arg1	magnesium					674:682	magnesium	674:682	magnesium	674:682	The mineral assay showed high amount of minerals, namely potassium, calcium, magnesium and zinc, which indicate that monguba seeds can be a new source of these minerals.
31108821	3	36	theme	monguba	333:339	arg1	seed					341:344	monguba seed	333:344	monguba seed	333:344	Nevertheless, the nutritional and functional potential of monguba seed is still underexploited.
31108821	7	37	theme	phenolic	855:862	arg1	compounds					864:872	the main phenolic compounds	846:872	the main phenolic compounds	846:872	UHPLC-ESI-MS/MS analysis showed caffeic, ferulic and 4-hydroxybenzoic acids as the main phenolic compounds, mainly in the esterified form, in these seeds.
31108821	7	37	theme	phenolic	855:862	arg1	acids					837:841	caffeic, ferulic and 4-hydroxybenzoic acids	799:841	caffeic, ferulic and 4-hydroxybenzoic acids	799:841	UHPLC-ESI-MS/MS analysis showed caffeic, ferulic and 4-hydroxybenzoic acids as the main phenolic compounds, mainly in the esterified form, in these seeds.
31108821	9	38	theme	oxygen	1140:1145	arg1	capacity					1166:1173	oxygen radical absorption capacity	1140:1173	oxygen radical absorption capacity (ORAC)	1140:1180	The soluble and insoluble phenolic fractions from monguba seeds showed high antioxidant activity measured by the oxygen radical absorption capacity (ORAC) and the trolox equivalent antioxidant capacity (TEAC) assays.
31108821	9	38	theme	oxygen	1140:1145	arg1	ORAC					1176:1179	ORAC	1176:1179	ORAC	1176:1179	The soluble and insoluble phenolic fractions from monguba seeds showed high antioxidant activity measured by the oxygen radical absorption capacity (ORAC) and the trolox equivalent antioxidant capacity (TEAC) assays.
31108821	2	39	theme	several	257:263	arg1	diseases					265:272	several diseases	257:272	several diseases	257:272	These nutritious seeds also have been used in popular medicine to treat several diseases.
31108821	3	40	theme	seed	341:344	arg1	potential					320:328	the nutritional and functional potential	289:328	the nutritional and functional potential of monguba seed	289:344	Nevertheless, the nutritional and functional potential of monguba seed is still underexploited.
31108821	4	41	theme	nutritional	406:416	arg1	components					433:442	the nutritional and functional components	402:442	the nutritional and functional components of monguba seeds	402:459	In this sense, we investigated the nutritional and functional components of monguba seeds.
31108821	0	42	dep	Pachira	58:64	arg1	aquatica					66:73	Pachira aquatica	58:73	Pachira aquatica	58:73	Chemical Composition and Antioxidant Activity of Monguba (Pachira aquatica) Seeds.
31108821	9	43	theme	absorption	1155:1164	arg1	capacity					1166:1173	oxygen radical absorption capacity	1140:1173	oxygen radical absorption capacity (ORAC)	1140:1180	The soluble and insoluble phenolic fractions from monguba seeds showed high antioxidant activity measured by the oxygen radical absorption capacity (ORAC) and the trolox equivalent antioxidant capacity (TEAC) assays.
31108821	9	43	theme	absorption	1155:1164	arg1	ORAC					1176:1179	ORAC	1176:1179	ORAC	1176:1179	The soluble and insoluble phenolic fractions from monguba seeds showed high antioxidant activity measured by the oxygen radical absorption capacity (ORAC) and the trolox equivalent antioxidant capacity (TEAC) assays.
31108821	6	44	theme	high	622:625	arg1	potassium					654:662	potassium	654:662	potassium	654:662	The mineral assay showed high amount of minerals, namely potassium, calcium, magnesium and zinc, which indicate that monguba seeds can be a new source of these minerals.
31108821	6	44	theme	high	622:625	arg1	zinc					688:691	zinc	688:691	zinc	688:691	The mineral assay showed high amount of minerals, namely potassium, calcium, magnesium and zinc, which indicate that monguba seeds can be a new source of these minerals.
31108821	6	44	theme	high	622:625	arg1	amount					627:632	high amount	622:632	high amount	622:632	The mineral assay showed high amount of minerals, namely potassium, calcium, magnesium and zinc, which indicate that monguba seeds can be a new source of these minerals.
31108821	6	44	theme	high	622:625	arg1	calcium					665:671	calcium	665:671	calcium	665:671	The mineral assay showed high amount of minerals, namely potassium, calcium, magnesium and zinc, which indicate that monguba seeds can be a new source of these minerals.
31108821	6	44	theme	high	622:625	arg1	minerals					637:644	minerals	637:644	minerals	637:644	The mineral assay showed high amount of minerals, namely potassium, calcium, magnesium and zinc, which indicate that monguba seeds can be a new source of these minerals.
31108821	6	44	theme	high	622:625	arg1	magnesium					674:682	magnesium	674:682	magnesium	674:682	The mineral assay showed high amount of minerals, namely potassium, calcium, magnesium and zinc, which indicate that monguba seeds can be a new source of these minerals.
31108821	0	45	theme	Activity	37:44	arg1	Pachira					58:64	Pachira	58:64	Pachira	58:64	Chemical Composition and Antioxidant Activity of Monguba (Pachira aquatica) Seeds.
31108821	0	45	theme	Activity	37:44	arg1	Seeds					76:80	Chemical Composition and Antioxidant Activity of Monguba (Pachira aquatica) Seeds	0:80	Chemical Composition and Antioxidant Activity of Monguba (Pachira aquatica) Seeds.	0:81	Chemical Composition and Antioxidant Activity of Monguba (Pachira aquatica) Seeds.
31108821	1	46	contain	has	97:99	arg1	fruit					91:95	Monguba fruit	83:95	Monguba fruit	83:95	Monguba fruit has a seed with a chestnut-like flavor that can be consumed boiled, fried, and roasted.
31108821	1	46	contain	has	97:99	arg2	seed					103:106	seed	103:106	seed	103:106	Monguba fruit has a seed with a chestnut-like flavor that can be consumed boiled, fried, and roasted.
31108821	8	47	theme	palmitic	995:1002	arg1	compounds					980:988	the main compounds	971:988	the main compounds	971:988	Monguba seed showed high lipid content, in which the main compounds were palmitic acid and γ-tocopherol.
31108821	8	47	theme	palmitic	995:1002	arg1	acid					1004:1007	palmitic acid	995:1007	palmitic acid	995:1007	Monguba seed showed high lipid content, in which the main compounds were palmitic acid and γ-tocopherol.
31108821	4	48	theme	functional	422:431	arg1	components					433:442	the nutritional and functional components	402:442	the nutritional and functional components of monguba seeds	402:459	In this sense, we investigated the nutritional and functional components of monguba seeds.
31108821	2	49	theme	nutritious	191:200	arg1	seeds					202:206	These nutritious seeds	185:206	These nutritious seeds	185:206	These nutritious seeds also have been used in popular medicine to treat several diseases.
31108821	5	50	theme	sugars	503:508	arg1	content					492:498	high total content	481:498	high total content of sugars, mainly sucrose	481:524	These seeds showed high total content of sugars, mainly sucrose, whereas the content of the raffinose family oligosaccharides was low.
31108821	9	51	theme	antioxidant	1103:1113	arg1	activity					1115:1122	high antioxidant activity	1098:1122	high antioxidant activity measured by the oxygen radical absorption capacity (ORAC) and the trolox equivalent antioxidant capacity (TEAC) assays	1098:1241	The soluble and insoluble phenolic fractions from monguba seeds showed high antioxidant activity measured by the oxygen radical absorption capacity (ORAC) and the trolox equivalent antioxidant capacity (TEAC) assays.
31108821	0	52	theme	Monguba	49:55	arg1	Pachira					58:64	Pachira	58:64	Pachira	58:64	Chemical Composition and Antioxidant Activity of Monguba (Pachira aquatica) Seeds.
31108821	0	52	theme	Monguba	49:55	arg1	Seeds					76:80	Chemical Composition and Antioxidant Activity of Monguba (Pachira aquatica) Seeds	0:80	Chemical Composition and Antioxidant Activity of Monguba (Pachira aquatica) Seeds.	0:81	Chemical Composition and Antioxidant Activity of Monguba (Pachira aquatica) Seeds.
31108821	10	53	contain	have	1273:1276	arg1	seeds					1267:1271	the monguba seeds	1255:1271	the monguba seeds	1255:1271	Therefore, the monguba seeds have great potential to be explored by food, pharmaceutical and cosmetic industries due to their chemical composition.
31108821	10	53	contain	have	1273:1276	arg2	potential					1284:1292	great potential	1278:1292	great potential	1278:1292	Therefore, the monguba seeds have great potential to be explored by food, pharmaceutical and cosmetic industries due to their chemical composition.
31108821	1	54	dep	consumed	148:155	arg1	boiled					157:162	boiled	157:162	can be consumed boiled	141:162	Monguba fruit has a seed with a chestnut-like flavor that can be consumed boiled, fried, and roasted.
31108821	9	55	theme	insoluble	1043:1051	arg1	fractions					1062:1070	The soluble and insoluble phenolic fractions	1027:1070	The soluble and insoluble phenolic fractions from monguba seeds	1027:1089	The soluble and insoluble phenolic fractions from monguba seeds showed high antioxidant activity measured by the oxygen radical absorption capacity (ORAC) and the trolox equivalent antioxidant capacity (TEAC) assays.
31108821	9	56	theme	capacity	1166:1173	arg1	assays					1236:1241	the oxygen radical absorption capacity (ORAC) and the trolox equivalent antioxidant capacity (TEAC) assays	1136:1241	the oxygen radical absorption capacity (ORAC) and the trolox equivalent antioxidant capacity (TEAC) assays	1136:1241	The soluble and insoluble phenolic fractions from monguba seeds showed high antioxidant activity measured by the oxygen radical absorption capacity (ORAC) and the trolox equivalent antioxidant capacity (TEAC) assays.
31108821	6	57	theme	minerals	757:764	arg1	seeds					722:726	monguba seeds	714:726	monguba seeds	714:726	The mineral assay showed high amount of minerals, namely potassium, calcium, magnesium and zinc, which indicate that monguba seeds can be a new source of these minerals.
31108821	6	57	theme	minerals	757:764	arg1	source					741:746	a new source	735:746	a new source of these minerals	735:764	The mineral assay showed high amount of minerals, namely potassium, calcium, magnesium and zinc, which indicate that monguba seeds can be a new source of these minerals.
31108821	9	58	theme	high	1098:1101	arg1	activity					1115:1122	high antioxidant activity	1098:1122	high antioxidant activity measured by the oxygen radical absorption capacity (ORAC) and the trolox equivalent antioxidant capacity (TEAC) assays	1098:1241	The soluble and insoluble phenolic fractions from monguba seeds showed high antioxidant activity measured by the oxygen radical absorption capacity (ORAC) and the trolox equivalent antioxidant capacity (TEAC) assays.
31108821	7	59	theme	main	850:853	arg1	compounds					864:872	the main phenolic compounds	846:872	the main phenolic compounds	846:872	UHPLC-ESI-MS/MS analysis showed caffeic, ferulic and 4-hydroxybenzoic acids as the main phenolic compounds, mainly in the esterified form, in these seeds.
31108821	7	59	theme	main	850:853	arg1	acids					837:841	caffeic, ferulic and 4-hydroxybenzoic acids	799:841	caffeic, ferulic and 4-hydroxybenzoic acids	799:841	UHPLC-ESI-MS/MS analysis showed caffeic, ferulic and 4-hydroxybenzoic acids as the main phenolic compounds, mainly in the esterified form, in these seeds.
31108821	8	60	from	acid	1004:1007	arg1	content					953:959	high lipid content	942:959	high lipid content	942:959	Monguba seed showed high lipid content, in which the main compounds were palmitic acid and γ-tocopherol.
31108821	9	61	theme	phenolic	1053:1060	arg1	fractions					1062:1070	The soluble and insoluble phenolic fractions	1027:1070	The soluble and insoluble phenolic fractions from monguba seeds	1027:1089	The soluble and insoluble phenolic fractions from monguba seeds showed high antioxidant activity measured by the oxygen radical absorption capacity (ORAC) and the trolox equivalent antioxidant capacity (TEAC) assays.
31108821	9	62	theme	soluble	1031:1037	arg1	fractions					1062:1070	The soluble and insoluble phenolic fractions	1027:1070	The soluble and insoluble phenolic fractions from monguba seeds	1027:1089	The soluble and insoluble phenolic fractions from monguba seeds showed high antioxidant activity measured by the oxygen radical absorption capacity (ORAC) and the trolox equivalent antioxidant capacity (TEAC) assays.
31108821	5	63	theme	high	481:484	arg1	content					492:498	high total content	481:498	high total content of sugars, mainly sucrose	481:524	These seeds showed high total content of sugars, mainly sucrose, whereas the content of the raffinose family oligosaccharides was low.
31108821	6	64	theme	mineral	601:607	arg1	assay					609:613	The mineral assay	597:613	The mineral assay	597:613	The mineral assay showed high amount of minerals, namely potassium, calcium, magnesium and zinc, which indicate that monguba seeds can be a new source of these minerals.
31108821	0	65	theme	Antioxidant	25:35	arg1	Activity					37:44	Antioxidant Activity	25:44	Antioxidant Activity	25:44	Chemical Composition and Antioxidant Activity of Monguba (Pachira aquatica) Seeds.
31108821	5	66	theme	total	486:490	arg1	content					492:498	high total content	481:498	high total content of sugars, mainly sucrose	481:524	These seeds showed high total content of sugars, mainly sucrose, whereas the content of the raffinose family oligosaccharides was low.
31108821	1	67	theme	chestnut-like	115:127	arg1	flavor					129:134	a chestnut-like flavor	113:134	a chestnut-like flavor that can be consumed boiled	113:162	Monguba fruit has a seed with a chestnut-like flavor that can be consumed boiled, fried, and roasted.
31108821	3	68	theme	nutritional	293:303	arg1	potential					320:328	the nutritional and functional potential	289:328	the nutritional and functional potential of monguba seed	289:344	Nevertheless, the nutritional and functional potential of monguba seed is still underexploited.
31108821	9	69	theme	trolox	1190:1195	arg1	TEAC					1230:1233	TEAC	1230:1233	TEAC	1230:1233	The soluble and insoluble phenolic fractions from monguba seeds showed high antioxidant activity measured by the oxygen radical absorption capacity (ORAC) and the trolox equivalent antioxidant capacity (TEAC) assays.
31108821	9	69	theme	trolox	1190:1195	arg1	capacity					1220:1227	the trolox equivalent antioxidant capacity	1186:1227	the trolox equivalent antioxidant capacity (TEAC)	1186:1234	The soluble and insoluble phenolic fractions from monguba seeds showed high antioxidant activity measured by the oxygen radical absorption capacity (ORAC) and the trolox equivalent antioxidant capacity (TEAC) assays.
31108821	9	70	theme	monguba	1077:1083	arg1	seeds					1085:1089	monguba seeds	1077:1089	monguba seeds	1077:1089	The soluble and insoluble phenolic fractions from monguba seeds showed high antioxidant activity measured by the oxygen radical absorption capacity (ORAC) and the trolox equivalent antioxidant capacity (TEAC) assays.
31472871	7	0	contain	has	1168:1170	arg2	potential					1176:1184	the potential	1172:1184	the potential as a bone repair material	1172:1210	All the results indicated that this hydrogel has the potential as a bone repair material.
31472871	7	0	contain	has	1168:1170	arg1	hydrogel					1159:1166	this hydrogel	1154:1166	this hydrogel	1154:1166	All the results indicated that this hydrogel has the potential as a bone repair material.
31472871	6	1	dep	in	942:943	arg1	vivo					945:948	vivo	945:948	vivo	945:948	The in vivo experiments revealed that the surface mineralized double network hydrogel significantly accelerated simultaneous regeneration of bone defects in a rabbit bone defect model.
31472871	3	2	theme	dissolution	503:513	arg1	system					515:520	KOH/urea dissolution system	494:520	KOH/urea dissolution system	494:520	Here, a new double network (DN) hydrogel was constructed by physical cross-linking of medical grade poly (vinyl alcohol) (PVA) and chitosan in KOH/urea dissolution system.
31472871	0	3	theme	bone	104:107	arg1	repair					109:114	bone repair	104:114	bone repair	104:114	Construction of physical-crosslink chitosan/PVA double-network hydrogel with surface mineralization for bone repair.
31472871	1	4	theme	formidable	195:204	arg1	obstruct					206:213	formidable obstruct	195:213	formidable obstruct	195:213	Weak mechanical properties, lack biocompatibility and relatively bioinert are formidable obstruct in application of bone repair materials.
31472871	1	4	theme	formidable	195:204	arg1	properties					133:142	Weak mechanical properties	117:142	Weak mechanical properties	117:142	Weak mechanical properties, lack biocompatibility and relatively bioinert are formidable obstruct in application of bone repair materials.
31472871	6	5	theme	defect	1109:1114	arg1	model					1116:1120	a rabbit bone defect model	1095:1120	a rabbit bone defect model	1095:1120	The in vivo experiments revealed that the surface mineralized double network hydrogel significantly accelerated simultaneous regeneration of bone defects in a rabbit bone defect model.
31472871	6	6	theme	network	1007:1013	arg1	hydrogel					1015:1022	the surface mineralized double network hydrogel	976:1022	the surface mineralized double network hydrogel	976:1022	The in vivo experiments revealed that the surface mineralized double network hydrogel significantly accelerated simultaneous regeneration of bone defects in a rabbit bone defect model.
31472871	4	7	theme	at	608:609	arg1	break					611:615	elongation at break	597:615	elongation at break (286%)	597:622	The obtained hydrogel demonstrated excellent tensile strength (0.24 MPa), elongation at break (286%), and high compressive strength (0.11 MPa on the strain of 60%).
31472871	4	7	theme	at	608:609	arg1	%					621:621	286%	618:621	286%	618:621	The obtained hydrogel demonstrated excellent tensile strength (0.24 MPa), elongation at break (286%), and high compressive strength (0.11 MPa on the strain of 60%).
31472871	6	8	theme	in	942:943	arg1	experiments					950:960	The in vivo experiments	938:960	The in vivo experiments	938:960	The in vivo experiments revealed that the surface mineralized double network hydrogel significantly accelerated simultaneous regeneration of bone defects in a rabbit bone defect model.
31472871	6	9	theme	defects	1084:1090	arg1	regeneration					1063:1074	simultaneous regeneration	1050:1074	simultaneous regeneration of bone defects	1050:1090	The in vivo experiments revealed that the surface mineralized double network hydrogel significantly accelerated simultaneous regeneration of bone defects in a rabbit bone defect model.
31472871	4	10	from	0.11 MPa	656:663	arg1	strain					672:677	the strain	668:677	the strain of 60%	668:684	The obtained hydrogel demonstrated excellent tensile strength (0.24 MPa), elongation at break (286%), and high compressive strength (0.11 MPa on the strain of 60%).
31472871	4	11	theme	elongation	597:606	arg1	break					611:615	elongation at break	597:615	elongation at break (286%)	597:622	The obtained hydrogel demonstrated excellent tensile strength (0.24 MPa), elongation at break (286%), and high compressive strength (0.11 MPa on the strain of 60%).
31472871	4	11	theme	elongation	597:606	arg1	%					621:621	286%	618:621	286%	618:621	The obtained hydrogel demonstrated excellent tensile strength (0.24 MPa), elongation at break (286%), and high compressive strength (0.11 MPa on the strain of 60%).
31472871	4	12	from	break	611:615	arg1	elongation					597:606	elongation at break	597:615	elongation at break (286%)	597:622	The obtained hydrogel demonstrated excellent tensile strength (0.24 MPa), elongation at break (286%), and high compressive strength (0.11 MPa on the strain of 60%).
31472871	4	12	from	break	611:615	arg1	at					608:609	elongation at break	597:615	elongation at break (286%)	597:622	The obtained hydrogel demonstrated excellent tensile strength (0.24 MPa), elongation at break (286%), and high compressive strength (0.11 MPa on the strain of 60%).
31472871	6	13	theme	simultaneous	1050:1061	arg1	regeneration					1063:1074	simultaneous regeneration	1050:1074	simultaneous regeneration of bone defects	1050:1090	The in vivo experiments revealed that the surface mineralized double network hydrogel significantly accelerated simultaneous regeneration of bone defects in a rabbit bone defect model.
31472871	3	14	theme	vinyl	457:461	arg1	poly					451:454	medical grade poly	437:454	medical grade poly (vinyl alcohol) (PVA)	437:476	Here, a new double network (DN) hydrogel was constructed by physical cross-linking of medical grade poly (vinyl alcohol) (PVA) and chitosan in KOH/urea dissolution system.
31472871	3	14	theme	vinyl	457:461	arg1	alcohol					463:469	vinyl alcohol	457:469	vinyl alcohol	457:469	Here, a new double network (DN) hydrogel was constructed by physical cross-linking of medical grade poly (vinyl alcohol) (PVA) and chitosan in KOH/urea dissolution system.
31472871	3	15	theme	DN	379:380	arg1	hydrogel					383:390	a new double network (DN) hydrogel	357:390	a new double network (DN) hydrogel	357:390	Here, a new double network (DN) hydrogel was constructed by physical cross-linking of medical grade poly (vinyl alcohol) (PVA) and chitosan in KOH/urea dissolution system.
31472871	5	16	theme	excellent	738:746	arg1	biocompatibility					748:763	excellent biocompatibility	738:763	excellent biocompatibility	738:763	Our studies showed that the prepared hydrogel had excellent biocompatibility in vitro and the introduction of hydroxyapatite (HAp) by surface mineralization imparted hydrogel the ability to induce rat bone marrow stem cells (rBMSCs) differentiation.
31472871	2	17	theme	viable	318:323	arg1	materials					282:290	Multifunctional composite materials	256:290	Multifunctional composite materials	256:290	Multifunctional composite materials have been considered as a viable solution to this problem.
31472871	2	17	theme	viable	318:323	arg1	solution					325:332	a viable solution	316:332	a viable solution to this problem	316:348	Multifunctional composite materials have been considered as a viable solution to this problem.
31472871	4	18	theme	compressive	634:644	arg1	strength					646:653	high compressive strength	629:653	high compressive strength (0.11 MPa on the strain of 60%)	629:685	The obtained hydrogel demonstrated excellent tensile strength (0.24 MPa), elongation at break (286%), and high compressive strength (0.11 MPa on the strain of 60%).
31472871	3	19	theme	new	359:361	arg1	hydrogel					383:390	a new double network (DN) hydrogel	357:390	a new double network (DN) hydrogel	357:390	Here, a new double network (DN) hydrogel was constructed by physical cross-linking of medical grade poly (vinyl alcohol) (PVA) and chitosan in KOH/urea dissolution system.
31472871	7	20	theme	bone	1191:1194	arg1	material					1203:1210	a bone repair material	1189:1210	a bone repair material	1189:1210	All the results indicated that this hydrogel has the potential as a bone repair material.
31472871	7	21	theme	repair	1196:1201	arg1	material					1203:1210	a bone repair material	1189:1210	a bone repair material	1189:1210	All the results indicated that this hydrogel has the potential as a bone repair material.
31472871	5	22	theme	stem	901:904	arg1	rBMSCs					913:918	rBMSCs	913:918	rBMSCs	913:918	Our studies showed that the prepared hydrogel had excellent biocompatibility in vitro and the introduction of hydroxyapatite (HAp) by surface mineralization imparted hydrogel the ability to induce rat bone marrow stem cells (rBMSCs) differentiation.
31472871	5	22	theme	stem	901:904	arg1	cells					906:910	rat bone marrow stem cells	885:910	rat bone marrow stem cells (rBMSCs) differentiation	885:935	Our studies showed that the prepared hydrogel had excellent biocompatibility in vitro and the introduction of hydroxyapatite (HAp) by surface mineralization imparted hydrogel the ability to induce rat bone marrow stem cells (rBMSCs) differentiation.
31472871	3	23	theme	poly	451:454	arg1	cross-linking					420:432	physical cross-linking	411:432	physical cross-linking of medical grade poly (vinyl alcohol) (PVA) and chitosan in KOH/urea dissolution system	411:520	Here, a new double network (DN) hydrogel was constructed by physical cross-linking of medical grade poly (vinyl alcohol) (PVA) and chitosan in KOH/urea dissolution system.
31472871	3	24	theme	physical	411:418	arg1	cross-linking					420:432	physical cross-linking	411:432	physical cross-linking of medical grade poly (vinyl alcohol) (PVA) and chitosan in KOH/urea dissolution system	411:520	Here, a new double network (DN) hydrogel was constructed by physical cross-linking of medical grade poly (vinyl alcohol) (PVA) and chitosan in KOH/urea dissolution system.
31472871	1	25	theme	Weak	117:120	arg1	biocompatibility					150:165	lack biocompatibility	145:165	lack biocompatibility	145:165	Weak mechanical properties, lack biocompatibility and relatively bioinert are formidable obstruct in application of bone repair materials.
31472871	1	25	theme	Weak	117:120	arg1	bioinert					182:189	bioinert	182:189	bioinert	182:189	Weak mechanical properties, lack biocompatibility and relatively bioinert are formidable obstruct in application of bone repair materials.
31472871	1	25	theme	Weak	117:120	arg1	obstruct					206:213	formidable obstruct	195:213	formidable obstruct	195:213	Weak mechanical properties, lack biocompatibility and relatively bioinert are formidable obstruct in application of bone repair materials.
31472871	1	25	theme	Weak	117:120	arg1	properties					133:142	Weak mechanical properties	117:142	Weak mechanical properties	117:142	Weak mechanical properties, lack biocompatibility and relatively bioinert are formidable obstruct in application of bone repair materials.
31472871	4	26	theme	tensile	568:574	arg1	strength					576:583	excellent tensile strength	558:583	excellent tensile strength (0.24 MPa)	558:594	The obtained hydrogel demonstrated excellent tensile strength (0.24 MPa), elongation at break (286%), and high compressive strength (0.11 MPa on the strain of 60%).
31472871	4	26	theme	tensile	568:574	arg1	0.24 MPa					586:593	0.24 MPa	586:593	0.24 MPa	586:593	The obtained hydrogel demonstrated excellent tensile strength (0.24 MPa), elongation at break (286%), and high compressive strength (0.11 MPa on the strain of 60%).
31472871	5	27	theme	marrow	894:899	arg1	rBMSCs					913:918	rBMSCs	913:918	rBMSCs	913:918	Our studies showed that the prepared hydrogel had excellent biocompatibility in vitro and the introduction of hydroxyapatite (HAp) by surface mineralization imparted hydrogel the ability to induce rat bone marrow stem cells (rBMSCs) differentiation.
31472871	5	27	theme	marrow	894:899	arg1	cells					906:910	rat bone marrow stem cells	885:910	rat bone marrow stem cells (rBMSCs) differentiation	885:935	Our studies showed that the prepared hydrogel had excellent biocompatibility in vitro and the introduction of hydroxyapatite (HAp) by surface mineralization imparted hydrogel the ability to induce rat bone marrow stem cells (rBMSCs) differentiation.
31472871	1	28	theme	mechanical	122:131	arg1	biocompatibility					150:165	lack biocompatibility	145:165	lack biocompatibility	145:165	Weak mechanical properties, lack biocompatibility and relatively bioinert are formidable obstruct in application of bone repair materials.
31472871	1	28	theme	mechanical	122:131	arg1	bioinert					182:189	bioinert	182:189	bioinert	182:189	Weak mechanical properties, lack biocompatibility and relatively bioinert are formidable obstruct in application of bone repair materials.
31472871	1	28	theme	mechanical	122:131	arg1	obstruct					206:213	formidable obstruct	195:213	formidable obstruct	195:213	Weak mechanical properties, lack biocompatibility and relatively bioinert are formidable obstruct in application of bone repair materials.
31472871	1	28	theme	mechanical	122:131	arg1	properties					133:142	Weak mechanical properties	117:142	Weak mechanical properties	117:142	Weak mechanical properties, lack biocompatibility and relatively bioinert are formidable obstruct in application of bone repair materials.
31472871	1	29	theme	bone	233:236	arg1	materials					245:253	bone repair materials	233:253	bone repair materials	233:253	Weak mechanical properties, lack biocompatibility and relatively bioinert are formidable obstruct in application of bone repair materials.
31472871	0	30	theme	physical-crosslink	16:33	arg1	Construction					0:11	Construction	0:11	Construction of physical-crosslink	0:33	Construction of physical-crosslink chitosan/PVA double-network hydrogel with surface mineralization for bone repair.
31472871	5	31	theme	cells	906:910	arg1	differentiation					921:935	rat bone marrow stem cells (rBMSCs) differentiation	885:935	rat bone marrow stem cells (rBMSCs) differentiation	885:935	Our studies showed that the prepared hydrogel had excellent biocompatibility in vitro and the introduction of hydroxyapatite (HAp) by surface mineralization imparted hydrogel the ability to induce rat bone marrow stem cells (rBMSCs) differentiation.
31472871	6	32	theme	bone	1079:1082	arg1	defects					1084:1090	bone defects	1079:1090	bone defects	1079:1090	The in vivo experiments revealed that the surface mineralized double network hydrogel significantly accelerated simultaneous regeneration of bone defects in a rabbit bone defect model.
31472871	1	33	theme	repair	238:243	arg1	materials					245:253	bone repair materials	233:253	bone repair materials	233:253	Weak mechanical properties, lack biocompatibility and relatively bioinert are formidable obstruct in application of bone repair materials.
31472871	3	34	theme	medical	437:443	arg1	poly					451:454	medical grade poly	437:454	medical grade poly (vinyl alcohol) (PVA)	437:476	Here, a new double network (DN) hydrogel was constructed by physical cross-linking of medical grade poly (vinyl alcohol) (PVA) and chitosan in KOH/urea dissolution system.
31472871	3	34	theme	medical	437:443	arg1	alcohol					463:469	vinyl alcohol	457:469	vinyl alcohol	457:469	Here, a new double network (DN) hydrogel was constructed by physical cross-linking of medical grade poly (vinyl alcohol) (PVA) and chitosan in KOH/urea dissolution system.
31472871	3	34	theme	medical	437:443	arg1	PVA					473:475	PVA	473:475	PVA	473:475	Here, a new double network (DN) hydrogel was constructed by physical cross-linking of medical grade poly (vinyl alcohol) (PVA) and chitosan in KOH/urea dissolution system.
31472871	3	35	theme	KOH/urea	494:501	arg1	system					515:520	KOH/urea dissolution system	494:520	KOH/urea dissolution system	494:520	Here, a new double network (DN) hydrogel was constructed by physical cross-linking of medical grade poly (vinyl alcohol) (PVA) and chitosan in KOH/urea dissolution system.
31472871	4	36	theme	high	629:632	arg1	strength					646:653	high compressive strength	629:653	high compressive strength (0.11 MPa on the strain of 60%)	629:685	The obtained hydrogel demonstrated excellent tensile strength (0.24 MPa), elongation at break (286%), and high compressive strength (0.11 MPa on the strain of 60%).
31472871	1	37	theme	materials	245:253	arg1	application					218:228	application	218:228	application of bone repair materials	218:253	Weak mechanical properties, lack biocompatibility and relatively bioinert are formidable obstruct in application of bone repair materials.
31472871	0	38	theme	double-network	48:61	arg1	hydrogel					63:70	double-network hydrogel	48:70	double-network hydrogel with surface mineralization for bone repair	48:114	Construction of physical-crosslink chitosan/PVA double-network hydrogel with surface mineralization for bone repair.
31472871	3	39	theme	grade	445:449	arg1	poly					451:454	medical grade poly	437:454	medical grade poly (vinyl alcohol) (PVA)	437:476	Here, a new double network (DN) hydrogel was constructed by physical cross-linking of medical grade poly (vinyl alcohol) (PVA) and chitosan in KOH/urea dissolution system.
31472871	3	39	theme	grade	445:449	arg1	alcohol					463:469	vinyl alcohol	457:469	vinyl alcohol	457:469	Here, a new double network (DN) hydrogel was constructed by physical cross-linking of medical grade poly (vinyl alcohol) (PVA) and chitosan in KOH/urea dissolution system.
31472871	3	39	theme	grade	445:449	arg1	PVA					473:475	PVA	473:475	PVA	473:475	Here, a new double network (DN) hydrogel was constructed by physical cross-linking of medical grade poly (vinyl alcohol) (PVA) and chitosan in KOH/urea dissolution system.
31472871	5	40	theme	rat	885:887	arg1	marrow					894:899	rat bone marrow	885:899	rat bone marrow stem cells (rBMSCs) differentiation	885:935	Our studies showed that the prepared hydrogel had excellent biocompatibility in vitro and the introduction of hydroxyapatite (HAp) by surface mineralization imparted hydrogel the ability to induce rat bone marrow stem cells (rBMSCs) differentiation.
31472871	6	41	theme	double	1000:1005	arg1	hydrogel					1015:1022	the surface mineralized double network hydrogel	976:1022	the surface mineralized double network hydrogel	976:1022	The in vivo experiments revealed that the surface mineralized double network hydrogel significantly accelerated simultaneous regeneration of bone defects in a rabbit bone defect model.
31472871	0	42	theme	surface	77:83	arg1	mineralization					85:98	surface mineralization	77:98	surface mineralization	77:98	Construction of physical-crosslink chitosan/PVA double-network hydrogel with surface mineralization for bone repair.
31472871	4	43	theme	obtained	527:534	arg1	hydrogel					536:543	The obtained hydrogel	523:543	The obtained hydrogel	523:543	The obtained hydrogel demonstrated excellent tensile strength (0.24 MPa), elongation at break (286%), and high compressive strength (0.11 MPa on the strain of 60%).
31472871	5	44	theme	prepared	716:723	arg1	hydrogel					725:732	the prepared hydrogel	712:732	the prepared hydrogel	712:732	Our studies showed that the prepared hydrogel had excellent biocompatibility in vitro and the introduction of hydroxyapatite (HAp) by surface mineralization imparted hydrogel the ability to induce rat bone marrow stem cells (rBMSCs) differentiation.
31472871	2	45	theme	composite	272:280	arg1	materials					282:290	Multifunctional composite materials	256:290	Multifunctional composite materials	256:290	Multifunctional composite materials have been considered as a viable solution to this problem.
31472871	2	45	theme	composite	272:280	arg1	solution					325:332	a viable solution	316:332	a viable solution to this problem	316:348	Multifunctional composite materials have been considered as a viable solution to this problem.
31472871	6	46	theme	bone	1104:1107	arg1	model					1116:1120	a rabbit bone defect model	1095:1120	a rabbit bone defect model	1095:1120	The in vivo experiments revealed that the surface mineralized double network hydrogel significantly accelerated simultaneous regeneration of bone defects in a rabbit bone defect model.
31472871	4	47	theme	%	684:684	arg1	strain					672:677	the strain	668:677	the strain of 60%	668:684	The obtained hydrogel demonstrated excellent tensile strength (0.24 MPa), elongation at break (286%), and high compressive strength (0.11 MPa on the strain of 60%).
31472871	4	48	theme	excellent	558:566	arg1	strength					576:583	excellent tensile strength	558:583	excellent tensile strength (0.24 MPa)	558:594	The obtained hydrogel demonstrated excellent tensile strength (0.24 MPa), elongation at break (286%), and high compressive strength (0.11 MPa on the strain of 60%).
31472871	4	48	theme	excellent	558:566	arg1	0.24 MPa					586:593	0.24 MPa	586:593	0.24 MPa	586:593	The obtained hydrogel demonstrated excellent tensile strength (0.24 MPa), elongation at break (286%), and high compressive strength (0.11 MPa on the strain of 60%).
31472871	5	49	contain	had	734:736	arg1	hydrogel					725:732	the prepared hydrogel	712:732	the prepared hydrogel	712:732	Our studies showed that the prepared hydrogel had excellent biocompatibility in vitro and the introduction of hydroxyapatite (HAp) by surface mineralization imparted hydrogel the ability to induce rat bone marrow stem cells (rBMSCs) differentiation.
31472871	5	49	contain	had	734:736	arg2	biocompatibility					748:763	excellent biocompatibility	738:763	excellent biocompatibility	738:763	Our studies showed that the prepared hydrogel had excellent biocompatibility in vitro and the introduction of hydroxyapatite (HAp) by surface mineralization imparted hydrogel the ability to induce rat bone marrow stem cells (rBMSCs) differentiation.
31472871	2	50	theme	Multifunctional	256:270	arg1	materials					282:290	Multifunctional composite materials	256:290	Multifunctional composite materials	256:290	Multifunctional composite materials have been considered as a viable solution to this problem.
31472871	2	50	theme	Multifunctional	256:270	arg1	solution					325:332	a viable solution	316:332	a viable solution to this problem	316:348	Multifunctional composite materials have been considered as a viable solution to this problem.
31472871	1	51	theme	lack	145:148	arg1	biocompatibility					150:165	lack biocompatibility	145:165	lack biocompatibility	145:165	Weak mechanical properties, lack biocompatibility and relatively bioinert are formidable obstruct in application of bone repair materials.
31472871	1	51	theme	lack	145:148	arg1	properties					133:142	Weak mechanical properties	117:142	Weak mechanical properties	117:142	Weak mechanical properties, lack biocompatibility and relatively bioinert are formidable obstruct in application of bone repair materials.
31472871	5	52	theme	bone	889:892	arg1	marrow					894:899	rat bone marrow	885:899	rat bone marrow stem cells (rBMSCs) differentiation	885:935	Our studies showed that the prepared hydrogel had excellent biocompatibility in vitro and the introduction of hydroxyapatite (HAp) by surface mineralization imparted hydrogel the ability to induce rat bone marrow stem cells (rBMSCs) differentiation.
31472871	0	53	with	hydrogel	63:70	arg1	mineralization					85:98	surface mineralization	77:98	surface mineralization	77:98	Construction of physical-crosslink chitosan/PVA double-network hydrogel with surface mineralization for bone repair.
31472871	6	54	theme	mineralized	988:998	arg1	hydrogel					1015:1022	the surface mineralized double network hydrogel	976:1022	the surface mineralized double network hydrogel	976:1022	The in vivo experiments revealed that the surface mineralized double network hydrogel significantly accelerated simultaneous regeneration of bone defects in a rabbit bone defect model.
31472871	3	55	theme	chitosan	482:489	arg1	cross-linking					420:432	physical cross-linking	411:432	physical cross-linking of medical grade poly (vinyl alcohol) (PVA) and chitosan in KOH/urea dissolution system	411:520	Here, a new double network (DN) hydrogel was constructed by physical cross-linking of medical grade poly (vinyl alcohol) (PVA) and chitosan in KOH/urea dissolution system.
31472871	6	56	theme	surface	980:986	arg1	hydrogel					1015:1022	the surface mineralized double network hydrogel	976:1022	the surface mineralized double network hydrogel	976:1022	The in vivo experiments revealed that the surface mineralized double network hydrogel significantly accelerated simultaneous regeneration of bone defects in a rabbit bone defect model.
31472871	3	57	theme	double	363:368	arg1	hydrogel					383:390	a new double network (DN) hydrogel	357:390	a new double network (DN) hydrogel	357:390	Here, a new double network (DN) hydrogel was constructed by physical cross-linking of medical grade poly (vinyl alcohol) (PVA) and chitosan in KOH/urea dissolution system.
31472871	4	58	dep	strength	646:653	arg1	0.11 MPa					656:663	0.11 MPa	656:663	0.11 MPa on the strain of 60%	656:684	The obtained hydrogel demonstrated excellent tensile strength (0.24 MPa), elongation at break (286%), and high compressive strength (0.11 MPa on the strain of 60%).
31472871	5	59	theme	hydroxyapatite	798:811	arg1	introduction					782:793	the introduction	778:793	the introduction of hydroxyapatite (HAp) by surface mineralization	778:843	Our studies showed that the prepared hydrogel had excellent biocompatibility in vitro and the introduction of hydroxyapatite (HAp) by surface mineralization imparted hydrogel the ability to induce rat bone marrow stem cells (rBMSCs) differentiation.
31472871	5	60	theme	surface	822:828	arg1	mineralization					830:843	surface mineralization	822:843	surface mineralization	822:843	Our studies showed that the prepared hydrogel had excellent biocompatibility in vitro and the introduction of hydroxyapatite (HAp) by surface mineralization imparted hydrogel the ability to induce rat bone marrow stem cells (rBMSCs) differentiation.
31472871	3	61	theme	network	370:376	arg1	hydrogel					383:390	a new double network (DN) hydrogel	357:390	a new double network (DN) hydrogel	357:390	Here, a new double network (DN) hydrogel was constructed by physical cross-linking of medical grade poly (vinyl alcohol) (PVA) and chitosan in KOH/urea dissolution system.
31472871	3	62	from	cross-linking	420:432	arg1	system					515:520	KOH/urea dissolution system	494:520	KOH/urea dissolution system	494:520	Here, a new double network (DN) hydrogel was constructed by physical cross-linking of medical grade poly (vinyl alcohol) (PVA) and chitosan in KOH/urea dissolution system.
31472871	6	63	theme	rabbit	1097:1102	arg1	model					1116:1120	a rabbit bone defect model	1095:1120	a rabbit bone defect model	1095:1120	The in vivo experiments revealed that the surface mineralized double network hydrogel significantly accelerated simultaneous regeneration of bone defects in a rabbit bone defect model.
31472871	1	64	from	obstruct	206:213	arg1	application					218:228	application	218:228	application of bone repair materials	218:253	Weak mechanical properties, lack biocompatibility and relatively bioinert are formidable obstruct in application of bone repair materials.
32254789	7	0	from	release	1169:1175	arg1	stages					1202:1207	both initial and late stages	1180:1207	stages	1202:1207	The two-phase delivery matrices display retarded FITC-BSA release in both initial and late stages compared to release from the PLGA microspheres or alginate fibre alone.
32254789	9	1	theme	novel	1585:1589	arg1	scaffolds					1600:1608	novel alginate scaffolds	1585:1608	novel alginate scaffolds with the ability to guide tissue regeneration through tightly-controlled release of one or more proteins in highly defined spatio-temporal configurations	1585:1762	The "programmable" microsphere concentration gradient fibre methodology presented here may enable development of novel alginate scaffolds with the ability to guide tissue regeneration through tightly-controlled release of one or more proteins in highly defined spatio-temporal configurations.
32254789	6	2	theme	fabrication	989:999	arg1	system					1001:1006	The fabrication system	985:1006	The fabrication system devised	985:1014	The fabrication system devised was shown to produce wet-spun fibres with a protein concentration gradient (G-Alg/PLGA fibre).
32254789	8	3	theme	microspheres	1356:1367	arg1	concentrations					1318:1331	higher concentrations	1311:1331	higher concentrations of protein-loaded PLGA microspheres	1311:1367	In addition, incorporation of higher concentrations of protein-loaded PLGA microspheres increased protein release compared to the fibres with lower concentrations of BSA-loaded microspheres.
32254789	2	4	theme	release	277:283	arg1	profiles					285:292	Specific bioactive molecule release profiles	249:292	Specific bioactive molecule release profiles	249:292	Specific bioactive molecule release profiles are achieved often by controlling polymer composition/concentration, which also determines the modulus of hydrogels.
32254789	1	5	theme	controlled	178:187	arg1	delivery					195:202	controlled local delivery	178:202	controlled local delivery of bioactive molecules in vitro and in vivo	178:246	Alginate has been a biologically viable option for controlled local delivery of bioactive molecules in vitro and in vivo.
32254789	9	6	from	release	1683:1689	arg1	configurations					1749:1762	highly defined spatio-temporal configurations	1718:1762	highly defined spatio-temporal configurations	1718:1762	The "programmable" microsphere concentration gradient fibre methodology presented here may enable development of novel alginate scaffolds with the ability to guide tissue regeneration through tightly-controlled release of one or more proteins in highly defined spatio-temporal configurations.
32254789	4	7	theme	poly-d	754:759	arg1	microspheres					794:805	protein-laden poly-d,l-lactic-co-glycolic acid (PLGA) microspheres	740:805	protein-laden poly-d,l-lactic-co-glycolic acid (PLGA) microspheres wet-spun into alginate fibres	740:835	Here we report a two-phase PLGA/alginate delivery system composed of protein-laden poly-d,l-lactic-co-glycolic acid (PLGA) microspheres wet-spun into alginate fibres.
32254789	7	8	theme	late	1197:1200	arg1	stages					1202:1207	both initial and late stages	1180:1207	stages	1202:1207	The two-phase delivery matrices display retarded FITC-BSA release in both initial and late stages compared to release from the PLGA microspheres or alginate fibre alone.
32254789	6	9	theme	concentration	1068:1080	arg1	G-Alg/PLGA					1092:1101	G-Alg/PLGA fibre	1092:1107	G-Alg/PLGA fibre	1092:1107	The fabrication system devised was shown to produce wet-spun fibres with a protein concentration gradient (G-Alg/PLGA fibre).
32254789	6	9	theme	concentration	1068:1080	arg1	gradient					1082:1089	a protein concentration gradient	1058:1089	a protein concentration gradient (G-Alg/PLGA fibre)	1058:1108	The fabrication system devised was shown to produce wet-spun fibres with a protein concentration gradient (G-Alg/PLGA fibre).
32254789	4	10	theme	PLGA/alginate	698:710	arg1	system					721:726	a two-phase PLGA/alginate delivery system	686:726	a two-phase PLGA/alginate delivery system composed of protein-laden poly-d,l-lactic-co-glycolic acid (PLGA) microspheres wet-spun into alginate fibres	686:835	Here we report a two-phase PLGA/alginate delivery system composed of protein-laden poly-d,l-lactic-co-glycolic acid (PLGA) microspheres wet-spun into alginate fibres.
32254789	7	11	theme	initial	1185:1191	arg1	stages					1202:1207	both initial and late stages	1180:1207	stages	1202:1207	The two-phase delivery matrices display retarded FITC-BSA release in both initial and late stages compared to release from the PLGA microspheres or alginate fibre alone.
32254789	1	12	theme	bioactive	207:215	arg1	molecules					217:225	bioactive molecules	207:225	bioactive molecules	207:225	Alginate has been a biologically viable option for controlled local delivery of bioactive molecules in vitro and in vivo.
32254789	8	13	theme	concentrations	1318:1331	arg1	incorporation					1294:1306	incorporation	1294:1306	incorporation of higher concentrations of protein-loaded PLGA microspheres	1294:1367	In addition, incorporation of higher concentrations of protein-loaded PLGA microspheres increased protein release compared to the fibres with lower concentrations of BSA-loaded microspheres.
32254789	7	14	theme	two-phase	1115:1123	arg1	matrices					1134:1141	The two-phase delivery matrices	1111:1141	The two-phase delivery matrices	1111:1141	The two-phase delivery matrices display retarded FITC-BSA release in both initial and late stages compared to release from the PLGA microspheres or alginate fibre alone.
32254789	9	15	theme	tissue	1636:1641	arg1	regeneration					1643:1654	tissue regeneration	1636:1654	tissue regeneration	1636:1654	The "programmable" microsphere concentration gradient fibre methodology presented here may enable development of novel alginate scaffolds with the ability to guide tissue regeneration through tightly-controlled release of one or more proteins in highly defined spatio-temporal configurations.
32254789	8	16	theme	protein-loaded	1336:1349	arg1	microspheres					1356:1367	protein-loaded PLGA microspheres	1336:1367	protein-loaded PLGA microspheres	1336:1367	In addition, incorporation of higher concentrations of protein-loaded PLGA microspheres increased protein release compared to the fibres with lower concentrations of BSA-loaded microspheres.
32254789	7	17	theme	FITC-BSA	1160:1167	arg1	release					1169:1175	retarded FITC-BSA release	1151:1175	retarded FITC-BSA release in both initial and late stages	1151:1207	The two-phase delivery matrices display retarded FITC-BSA release in both initial and late stages compared to release from the PLGA microspheres or alginate fibre alone.
32254789	3	18	theme	chemotactic	649:659	arg1	purposes					661:668	chemotactic purposes	649:668	chemotactic purposes	649:668	This largely limits alginate-mediated bioactive molecule delivery to single-factors of uniform concentration applications, rather than applications that may require (multiple) bioactive molecules delivered at a concentration gradient for chemotactic purposes.
32254789	2	19	theme	bioactive	258:266	arg1	profiles					285:292	Specific bioactive molecule release profiles	249:292	Specific bioactive molecule release profiles	249:292	Specific bioactive molecule release profiles are achieved often by controlling polymer composition/concentration, which also determines the modulus of hydrogels.
32254789	8	20	theme	BSA-loaded	1447:1456	arg1	microspheres					1458:1469	BSA-loaded microspheres	1447:1469	BSA-loaded microspheres	1447:1469	In addition, incorporation of higher concentrations of protein-loaded PLGA microspheres increased protein release compared to the fibres with lower concentrations of BSA-loaded microspheres.
32254789	5	21	theme	isothiocyanate-conjugated	850:874	arg1	FITC-BSA					898:905	FITC-BSA	898:905	FITC-BSA	898:905	Fluorescein isothiocyanate-conjugated bovine serum albumin (FITC-BSA) was used as a model protein and the developed structures were characterized.
32254789	5	21	theme	isothiocyanate-conjugated	850:874	arg1	albumin					889:895	Fluorescein isothiocyanate-conjugated bovine serum albumin	838:895	Fluorescein isothiocyanate-conjugated bovine serum albumin (FITC-BSA)	838:906	Fluorescein isothiocyanate-conjugated bovine serum albumin (FITC-BSA) was used as a model protein and the developed structures were characterized.
32254789	5	21	theme	isothiocyanate-conjugated	850:874	arg1	protein					928:934	a model protein	920:934	a model protein	920:934	Fluorescein isothiocyanate-conjugated bovine serum albumin (FITC-BSA) was used as a model protein and the developed structures were characterized.
32254789	7	22	from	microspheres	1243:1254	arg1	release					1221:1227	release	1221:1227	release from the PLGA microspheres or alginate fibre alone	1221:1278	The two-phase delivery matrices display retarded FITC-BSA release in both initial and late stages compared to release from the PLGA microspheres or alginate fibre alone.
32254789	0	23	theme	concentration	75:87	arg1	gradients					89:97	microsphere concentration gradients	63:97	microsphere concentration gradients for local protein delivery	63:124	A simple technique for development of fibres with programmable microsphere concentration gradients for local protein delivery.
32254789	9	24	from	proteins	1706:1713	arg1	configurations					1749:1762	highly defined spatio-temporal configurations	1718:1762	highly defined spatio-temporal configurations	1718:1762	The "programmable" microsphere concentration gradient fibre methodology presented here may enable development of novel alginate scaffolds with the ability to guide tissue regeneration through tightly-controlled release of one or more proteins in highly defined spatio-temporal configurations.
32254789	6	25	theme	fibre	1103:1107	arg1	G-Alg/PLGA					1092:1101	G-Alg/PLGA fibre	1092:1107	G-Alg/PLGA fibre	1092:1107	The fabrication system devised was shown to produce wet-spun fibres with a protein concentration gradient (G-Alg/PLGA fibre).
32254789	6	25	theme	fibre	1103:1107	arg1	gradient					1082:1089	a protein concentration gradient	1058:1089	a protein concentration gradient (G-Alg/PLGA fibre)	1058:1108	The fabrication system devised was shown to produce wet-spun fibres with a protein concentration gradient (G-Alg/PLGA fibre).
32254789	0	26	theme	protein	109:115	arg1	delivery					117:124	local protein delivery	103:124	local protein delivery	103:124	A simple technique for development of fibres with programmable microsphere concentration gradients for local protein delivery.
32254789	9	27	theme	defined	1725:1731	arg1	configurations					1749:1762	highly defined spatio-temporal configurations	1718:1762	highly defined spatio-temporal configurations	1718:1762	The "programmable" microsphere concentration gradient fibre methodology presented here may enable development of novel alginate scaffolds with the ability to guide tissue regeneration through tightly-controlled release of one or more proteins in highly defined spatio-temporal configurations.
32254789	0	28	theme	microsphere	63:73	arg1	gradients					89:97	microsphere concentration gradients	63:97	microsphere concentration gradients for local protein delivery	63:124	A simple technique for development of fibres with programmable microsphere concentration gradients for local protein delivery.
32254789	4	29	theme	alginate	821:828	arg1	fibres					830:835	alginate fibres	821:835	alginate fibres	821:835	Here we report a two-phase PLGA/alginate delivery system composed of protein-laden poly-d,l-lactic-co-glycolic acid (PLGA) microspheres wet-spun into alginate fibres.
32254789	4	30	dep	microspheres	794:805	arg1	wet-spun					807:814	wet-spun	807:814	wet-spun	807:814	Here we report a two-phase PLGA/alginate delivery system composed of protein-laden poly-d,l-lactic-co-glycolic acid (PLGA) microspheres wet-spun into alginate fibres.
32254789	3	31	theme	applications	520:531	arg1	single-factors					480:493	single-factors	480:493	single-factors of uniform concentration applications, rather than applications that may require (multiple) bioactive molecules delivered at a concentration gradient for chemotactic purposes	480:668	This largely limits alginate-mediated bioactive molecule delivery to single-factors of uniform concentration applications, rather than applications that may require (multiple) bioactive molecules delivered at a concentration gradient for chemotactic purposes.
32254789	9	32	theme	programmable	1477:1488	arg1	gradient					1517:1524	The "programmable" microsphere concentration gradient	1472:1524	The "programmable" microsphere concentration gradient fibre methodology presented here	1472:1557	The "programmable" microsphere concentration gradient fibre methodology presented here may enable development of novel alginate scaffolds with the ability to guide tissue regeneration through tightly-controlled release of one or more proteins in highly defined spatio-temporal configurations.
32254789	3	33	theme	bioactive	449:457	arg1	delivery					468:475	alginate-mediated bioactive molecule delivery	431:475	alginate-mediated bioactive molecule delivery	431:475	This largely limits alginate-mediated bioactive molecule delivery to single-factors of uniform concentration applications, rather than applications that may require (multiple) bioactive molecules delivered at a concentration gradient for chemotactic purposes.
32254789	3	34	theme	uniform	498:504	arg1	applications					520:531	uniform concentration applications	498:531	uniform concentration applications	498:531	This largely limits alginate-mediated bioactive molecule delivery to single-factors of uniform concentration applications, rather than applications that may require (multiple) bioactive molecules delivered at a concentration gradient for chemotactic purposes.
32254789	9	35	theme	alginate	1591:1598	arg1	scaffolds					1600:1608	novel alginate scaffolds	1585:1608	novel alginate scaffolds with the ability to guide tissue regeneration through tightly-controlled release of one or more proteins in highly defined spatio-temporal configurations	1585:1762	The "programmable" microsphere concentration gradient fibre methodology presented here may enable development of novel alginate scaffolds with the ability to guide tissue regeneration through tightly-controlled release of one or more proteins in highly defined spatio-temporal configurations.
32254789	9	36	theme	microsphere	1491:1501	arg1	gradient					1517:1524	The "programmable" microsphere concentration gradient	1472:1524	The "programmable" microsphere concentration gradient fibre methodology presented here	1472:1557	The "programmable" microsphere concentration gradient fibre methodology presented here may enable development of novel alginate scaffolds with the ability to guide tissue regeneration through tightly-controlled release of one or more proteins in highly defined spatio-temporal configurations.
32254789	8	37	with	fibres	1411:1416	arg1	concentrations					1429:1442	lower concentrations	1423:1442	lower concentrations of BSA-loaded microspheres	1423:1469	In addition, incorporation of higher concentrations of protein-loaded PLGA microspheres increased protein release compared to the fibres with lower concentrations of BSA-loaded microspheres.
32254789	3	38	theme	applications	546:557	arg1	single-factors					480:493	single-factors	480:493	single-factors of uniform concentration applications, rather than applications that may require (multiple) bioactive molecules delivered at a concentration gradient for chemotactic purposes	480:668	This largely limits alginate-mediated bioactive molecule delivery to single-factors of uniform concentration applications, rather than applications that may require (multiple) bioactive molecules delivered at a concentration gradient for chemotactic purposes.
32254789	0	39	theme	fibres	38:43	arg1	development					23:33	development	23:33	development of fibres with	23:48	A simple technique for development of fibres with programmable microsphere concentration gradients for local protein delivery.
32254789	9	40	theme	gradient	1517:1524	arg1	methodology					1532:1542	The "programmable" microsphere concentration gradient fibre methodology	1472:1542	The "programmable" microsphere concentration gradient fibre methodology presented here	1472:1557	The "programmable" microsphere concentration gradient fibre methodology presented here may enable development of novel alginate scaffolds with the ability to guide tissue regeneration through tightly-controlled release of one or more proteins in highly defined spatio-temporal configurations.
32254789	3	41	theme	bioactive	587:595	arg1	molecules					597:605	(multiple) bioactive molecules	576:605	(multiple) bioactive molecules delivered at a concentration gradient for chemotactic purposes	576:668	This largely limits alginate-mediated bioactive molecule delivery to single-factors of uniform concentration applications, rather than applications that may require (multiple) bioactive molecules delivered at a concentration gradient for chemotactic purposes.
32254789	9	42	theme	scaffolds	1600:1608	arg1	development					1570:1580	development	1570:1580	development of novel alginate scaffolds with the ability to guide tissue regeneration through tightly-controlled release of one or more proteins in highly defined spatio-temporal configurations	1570:1762	The "programmable" microsphere concentration gradient fibre methodology presented here may enable development of novel alginate scaffolds with the ability to guide tissue regeneration through tightly-controlled release of one or more proteins in highly defined spatio-temporal configurations.
32254789	2	43	theme	polymer	328:334	arg1	composition/concentration					336:360	polymer composition/concentration	328:360	polymer composition/concentration	328:360	Specific bioactive molecule release profiles are achieved often by controlling polymer composition/concentration, which also determines the modulus of hydrogels.
32254789	8	44	theme	protein	1379:1385	arg1	release					1387:1393	protein release	1379:1393	protein release	1379:1393	In addition, incorporation of higher concentrations of protein-loaded PLGA microspheres increased protein release compared to the fibres with lower concentrations of BSA-loaded microspheres.
32254789	5	45	theme	developed	944:952	arg1	structures					954:963	the developed structures	940:963	the developed structures	940:963	Fluorescein isothiocyanate-conjugated bovine serum albumin (FITC-BSA) was used as a model protein and the developed structures were characterized.
32254789	8	46	theme	PLGA	1351:1354	arg1	microspheres					1356:1367	protein-loaded PLGA microspheres	1336:1367	protein-loaded PLGA microspheres	1336:1367	In addition, incorporation of higher concentrations of protein-loaded PLGA microspheres increased protein release compared to the fibres with lower concentrations of BSA-loaded microspheres.
32254789	5	47	theme	serum	883:887	arg1	FITC-BSA					898:905	FITC-BSA	898:905	FITC-BSA	898:905	Fluorescein isothiocyanate-conjugated bovine serum albumin (FITC-BSA) was used as a model protein and the developed structures were characterized.
32254789	5	47	theme	serum	883:887	arg1	albumin					889:895	Fluorescein isothiocyanate-conjugated bovine serum albumin	838:895	Fluorescein isothiocyanate-conjugated bovine serum albumin (FITC-BSA)	838:906	Fluorescein isothiocyanate-conjugated bovine serum albumin (FITC-BSA) was used as a model protein and the developed structures were characterized.
32254789	5	47	theme	serum	883:887	arg1	protein					928:934	a model protein	920:934	a model protein	920:934	Fluorescein isothiocyanate-conjugated bovine serum albumin (FITC-BSA) was used as a model protein and the developed structures were characterized.
32254789	3	48	theme	concentration	622:634	arg1	gradient					636:643	a concentration gradient	620:643	a concentration gradient for chemotactic purposes	620:668	This largely limits alginate-mediated bioactive molecule delivery to single-factors of uniform concentration applications, rather than applications that may require (multiple) bioactive molecules delivered at a concentration gradient for chemotactic purposes.
32254789	1	49	theme	local	189:193	arg1	delivery					195:202	controlled local delivery	178:202	controlled local delivery of bioactive molecules in vitro and in vivo	178:246	Alginate has been a biologically viable option for controlled local delivery of bioactive molecules in vitro and in vivo.
32254789	4	50	theme	protein-laden	740:752	arg1	microspheres					794:805	protein-laden poly-d,l-lactic-co-glycolic acid (PLGA) microspheres	740:805	protein-laden poly-d,l-lactic-co-glycolic acid (PLGA) microspheres wet-spun into alginate fibres	740:835	Here we report a two-phase PLGA/alginate delivery system composed of protein-laden poly-d,l-lactic-co-glycolic acid (PLGA) microspheres wet-spun into alginate fibres.
32254789	5	51	theme	model	922:926	arg1	albumin					889:895	Fluorescein isothiocyanate-conjugated bovine serum albumin	838:895	Fluorescein isothiocyanate-conjugated bovine serum albumin (FITC-BSA)	838:906	Fluorescein isothiocyanate-conjugated bovine serum albumin (FITC-BSA) was used as a model protein and the developed structures were characterized.
32254789	5	51	theme	model	922:926	arg1	protein					928:934	a model protein	920:934	a model protein	920:934	Fluorescein isothiocyanate-conjugated bovine serum albumin (FITC-BSA) was used as a model protein and the developed structures were characterized.
32254789	6	52	theme	protein	1060:1066	arg1	G-Alg/PLGA					1092:1101	G-Alg/PLGA fibre	1092:1107	G-Alg/PLGA fibre	1092:1107	The fabrication system devised was shown to produce wet-spun fibres with a protein concentration gradient (G-Alg/PLGA fibre).
32254789	6	52	theme	protein	1060:1066	arg1	gradient					1082:1089	a protein concentration gradient	1058:1089	a protein concentration gradient (G-Alg/PLGA fibre)	1058:1108	The fabrication system devised was shown to produce wet-spun fibres with a protein concentration gradient (G-Alg/PLGA fibre).
32254789	8	53	theme	microspheres	1458:1469	arg1	concentrations					1429:1442	lower concentrations	1423:1442	lower concentrations of BSA-loaded microspheres	1423:1469	In addition, incorporation of higher concentrations of protein-loaded PLGA microspheres increased protein release compared to the fibres with lower concentrations of BSA-loaded microspheres.
32254789	4	54	theme	two-phase	688:696	arg1	system					721:726	a two-phase PLGA/alginate delivery system	686:726	a two-phase PLGA/alginate delivery system composed of protein-laden poly-d,l-lactic-co-glycolic acid (PLGA) microspheres wet-spun into alginate fibres	686:835	Here we report a two-phase PLGA/alginate delivery system composed of protein-laden poly-d,l-lactic-co-glycolic acid (PLGA) microspheres wet-spun into alginate fibres.
32254789	1	55	theme	molecules	217:225	arg1	delivery					195:202	controlled local delivery	178:202	controlled local delivery of bioactive molecules in vitro and in vivo	178:246	Alginate has been a biologically viable option for controlled local delivery of bioactive molecules in vitro and in vivo.
32254789	8	56	theme	higher	1311:1316	arg1	concentrations					1318:1331	higher concentrations	1311:1331	higher concentrations of protein-loaded PLGA microspheres	1311:1367	In addition, incorporation of higher concentrations of protein-loaded PLGA microspheres increased protein release compared to the fibres with lower concentrations of BSA-loaded microspheres.
32254789	4	57	theme	delivery	712:719	arg1	system					721:726	a two-phase PLGA/alginate delivery system	686:726	a two-phase PLGA/alginate delivery system composed of protein-laden poly-d,l-lactic-co-glycolic acid (PLGA) microspheres wet-spun into alginate fibres	686:835	Here we report a two-phase PLGA/alginate delivery system composed of protein-laden poly-d,l-lactic-co-glycolic acid (PLGA) microspheres wet-spun into alginate fibres.
32254789	7	58	theme	retarded	1151:1158	arg1	release					1169:1175	retarded FITC-BSA release	1151:1175	retarded FITC-BSA release in both initial and late stages	1151:1207	The two-phase delivery matrices display retarded FITC-BSA release in both initial and late stages compared to release from the PLGA microspheres or alginate fibre alone.
32254789	7	59	theme	delivery	1125:1132	arg1	matrices					1134:1141	The two-phase delivery matrices	1111:1141	The two-phase delivery matrices	1111:1141	The two-phase delivery matrices display retarded FITC-BSA release in both initial and late stages compared to release from the PLGA microspheres or alginate fibre alone.
32254789	6	60	theme	wet-spun	1037:1044	arg1	fibres					1046:1051	wet-spun fibres	1037:1051	wet-spun fibres with a protein concentration gradient (G-Alg/PLGA fibre)	1037:1108	The fabrication system devised was shown to produce wet-spun fibres with a protein concentration gradient (G-Alg/PLGA fibre).
32254789	4	61	theme	PLGA	788:791	arg1	microspheres					794:805	protein-laden poly-d,l-lactic-co-glycolic acid (PLGA) microspheres	740:805	protein-laden poly-d,l-lactic-co-glycolic acid (PLGA) microspheres wet-spun into alginate fibres	740:835	Here we report a two-phase PLGA/alginate delivery system composed of protein-laden poly-d,l-lactic-co-glycolic acid (PLGA) microspheres wet-spun into alginate fibres.
32254789	2	62	theme	molecule	268:275	arg1	profiles					285:292	Specific bioactive molecule release profiles	249:292	Specific bioactive molecule release profiles	249:292	Specific bioactive molecule release profiles are achieved often by controlling polymer composition/concentration, which also determines the modulus of hydrogels.
32254789	9	63	theme	tightly-controlled	1664:1681	arg1	release					1683:1689	tightly-controlled release	1664:1689	tightly-controlled release of one or more proteins in highly defined spatio-temporal configurations	1664:1762	The "programmable" microsphere concentration gradient fibre methodology presented here may enable development of novel alginate scaffolds with the ability to guide tissue regeneration through tightly-controlled release of one or more proteins in highly defined spatio-temporal configurations.
32254789	8	64	theme	lower	1423:1427	arg1	concentrations					1429:1442	lower concentrations	1423:1442	lower concentrations of BSA-loaded microspheres	1423:1469	In addition, incorporation of higher concentrations of protein-loaded PLGA microspheres increased protein release compared to the fibres with lower concentrations of BSA-loaded microspheres.
32254789	5	65	theme	Fluorescein	838:848	arg1	FITC-BSA					898:905	FITC-BSA	898:905	FITC-BSA	898:905	Fluorescein isothiocyanate-conjugated bovine serum albumin (FITC-BSA) was used as a model protein and the developed structures were characterized.
32254789	5	65	theme	Fluorescein	838:848	arg1	albumin					889:895	Fluorescein isothiocyanate-conjugated bovine serum albumin	838:895	Fluorescein isothiocyanate-conjugated bovine serum albumin (FITC-BSA)	838:906	Fluorescein isothiocyanate-conjugated bovine serum albumin (FITC-BSA) was used as a model protein and the developed structures were characterized.
32254789	5	65	theme	Fluorescein	838:848	arg1	protein					928:934	a model protein	920:934	a model protein	920:934	Fluorescein isothiocyanate-conjugated bovine serum albumin (FITC-BSA) was used as a model protein and the developed structures were characterized.
32254789	2	66	theme	Specific	249:256	arg1	profiles					285:292	Specific bioactive molecule release profiles	249:292	Specific bioactive molecule release profiles	249:292	Specific bioactive molecule release profiles are achieved often by controlling polymer composition/concentration, which also determines the modulus of hydrogels.
32254789	9	67	from	configurations	1749:1762	arg1	release					1683:1689	tightly-controlled release	1664:1689	tightly-controlled release of one or more proteins in highly defined spatio-temporal configurations	1664:1762	The "programmable" microsphere concentration gradient fibre methodology presented here may enable development of novel alginate scaffolds with the ability to guide tissue regeneration through tightly-controlled release of one or more proteins in highly defined spatio-temporal configurations.
32254789	5	68	theme	bovine	876:881	arg1	FITC-BSA					898:905	FITC-BSA	898:905	FITC-BSA	898:905	Fluorescein isothiocyanate-conjugated bovine serum albumin (FITC-BSA) was used as a model protein and the developed structures were characterized.
32254789	5	68	theme	bovine	876:881	arg1	albumin					889:895	Fluorescein isothiocyanate-conjugated bovine serum albumin	838:895	Fluorescein isothiocyanate-conjugated bovine serum albumin (FITC-BSA)	838:906	Fluorescein isothiocyanate-conjugated bovine serum albumin (FITC-BSA) was used as a model protein and the developed structures were characterized.
32254789	5	68	theme	bovine	876:881	arg1	protein					928:934	a model protein	920:934	a model protein	920:934	Fluorescein isothiocyanate-conjugated bovine serum albumin (FITC-BSA) was used as a model protein and the developed structures were characterized.
32254789	1	69	theme	viable	160:165	arg1	Alginate					127:134	Alginate	127:134	Alginate	127:134	Alginate has been a biologically viable option for controlled local delivery of bioactive molecules in vitro and in vivo.
32254789	1	69	theme	viable	160:165	arg1	option					167:172	a biologically viable option	145:172	a biologically viable option for controlled local delivery of bioactive molecules in vitro and in vivo	145:246	Alginate has been a biologically viable option for controlled local delivery of bioactive molecules in vitro and in vivo.
32254789	4	70	theme	acid	782:785	arg1	microspheres					794:805	protein-laden poly-d,l-lactic-co-glycolic acid (PLGA) microspheres	740:805	protein-laden poly-d,l-lactic-co-glycolic acid (PLGA) microspheres wet-spun into alginate fibres	740:835	Here we report a two-phase PLGA/alginate delivery system composed of protein-laden poly-d,l-lactic-co-glycolic acid (PLGA) microspheres wet-spun into alginate fibres.
32254789	0	71	theme	local	103:107	arg1	delivery					117:124	local protein delivery	103:124	local protein delivery	103:124	A simple technique for development of fibres with programmable microsphere concentration gradients for local protein delivery.
32254789	9	72	theme	proteins	1706:1713	arg1	release					1683:1689	tightly-controlled release	1664:1689	tightly-controlled release of one or more proteins in highly defined spatio-temporal configurations	1664:1762	The "programmable" microsphere concentration gradient fibre methodology presented here may enable development of novel alginate scaffolds with the ability to guide tissue regeneration through tightly-controlled release of one or more proteins in highly defined spatio-temporal configurations.
32254789	6	73	with	fibres	1046:1051	arg1	G-Alg/PLGA					1092:1101	G-Alg/PLGA fibre	1092:1107	G-Alg/PLGA fibre	1092:1107	The fabrication system devised was shown to produce wet-spun fibres with a protein concentration gradient (G-Alg/PLGA fibre).
32254789	6	73	with	fibres	1046:1051	arg1	gradient					1082:1089	a protein concentration gradient	1058:1089	a protein concentration gradient (G-Alg/PLGA fibre)	1058:1108	The fabrication system devised was shown to produce wet-spun fibres with a protein concentration gradient (G-Alg/PLGA fibre).
32254789	7	74	from	fibre	1268:1272	arg1	release					1221:1227	release	1221:1227	release from the PLGA microspheres or alginate fibre alone	1221:1278	The two-phase delivery matrices display retarded FITC-BSA release in both initial and late stages compared to release from the PLGA microspheres or alginate fibre alone.
32254789	0	75	theme	simple	2:7	arg1	technique					9:17	A simple technique	0:17	A simple technique for development of fibres with	0:48	A simple technique for development of fibres with programmable microsphere concentration gradients for local protein delivery.
32254789	9	76	theme	spatio-temporal	1733:1747	arg1	configurations					1749:1762	highly defined spatio-temporal configurations	1718:1762	highly defined spatio-temporal configurations	1718:1762	The "programmable" microsphere concentration gradient fibre methodology presented here may enable development of novel alginate scaffolds with the ability to guide tissue regeneration through tightly-controlled release of one or more proteins in highly defined spatio-temporal configurations.
32254789	2	77	theme	hydrogels	400:408	arg1	modulus					389:395	the modulus	385:395	the modulus of hydrogels	385:408	Specific bioactive molecule release profiles are achieved often by controlling polymer composition/concentration, which also determines the modulus of hydrogels.
32254789	9	78	theme	"	1489:1489	arg1	gradient					1517:1524	The "programmable" microsphere concentration gradient	1472:1524	The "programmable" microsphere concentration gradient fibre methodology presented here	1472:1557	The "programmable" microsphere concentration gradient fibre methodology presented here may enable development of novel alginate scaffolds with the ability to guide tissue regeneration through tightly-controlled release of one or more proteins in highly defined spatio-temporal configurations.
32254789	3	79	theme	molecule	459:466	arg1	delivery					468:475	alginate-mediated bioactive molecule delivery	431:475	alginate-mediated bioactive molecule delivery	431:475	This largely limits alginate-mediated bioactive molecule delivery to single-factors of uniform concentration applications, rather than applications that may require (multiple) bioactive molecules delivered at a concentration gradient for chemotactic purposes.
32254789	3	80	theme	concentration	506:518	arg1	applications					520:531	uniform concentration applications	498:531	uniform concentration applications	498:531	This largely limits alginate-mediated bioactive molecule delivery to single-factors of uniform concentration applications, rather than applications that may require (multiple) bioactive molecules delivered at a concentration gradient for chemotactic purposes.
32254789	7	81	theme	PLGA	1238:1241	arg1	microspheres					1243:1254	the PLGA microspheres	1234:1254	the PLGA microspheres	1234:1254	The two-phase delivery matrices display retarded FITC-BSA release in both initial and late stages compared to release from the PLGA microspheres or alginate fibre alone.
32254789	9	82	theme	concentration	1503:1515	arg1	gradient					1517:1524	The "programmable" microsphere concentration gradient	1472:1524	The "programmable" microsphere concentration gradient fibre methodology presented here	1472:1557	The "programmable" microsphere concentration gradient fibre methodology presented here may enable development of novel alginate scaffolds with the ability to guide tissue regeneration through tightly-controlled release of one or more proteins in highly defined spatio-temporal configurations.
32254789	3	83	theme	alginate-mediated	431:447	arg1	delivery					468:475	alginate-mediated bioactive molecule delivery	431:475	alginate-mediated bioactive molecule delivery	431:475	This largely limits alginate-mediated bioactive molecule delivery to single-factors of uniform concentration applications, rather than applications that may require (multiple) bioactive molecules delivered at a concentration gradient for chemotactic purposes.
32254789	9	84	theme	fibre	1526:1530	arg1	methodology					1532:1542	The "programmable" microsphere concentration gradient fibre methodology	1472:1542	The "programmable" microsphere concentration gradient fibre methodology presented here	1472:1557	The "programmable" microsphere concentration gradient fibre methodology presented here may enable development of novel alginate scaffolds with the ability to guide tissue regeneration through tightly-controlled release of one or more proteins in highly defined spatio-temporal configurations.
32254789	5	85	used	used	912:915	arg2	albumin					889:895	Fluorescein isothiocyanate-conjugated bovine serum albumin	838:895	Fluorescein isothiocyanate-conjugated bovine serum albumin (FITC-BSA)	838:906	Fluorescein isothiocyanate-conjugated bovine serum albumin (FITC-BSA) was used as a model protein and the developed structures were characterized.
32254789	5	85	used	used	912:915	arg2	protein					928:934	a model protein	920:934	a model protein	920:934	Fluorescein isothiocyanate-conjugated bovine serum albumin (FITC-BSA) was used as a model protein and the developed structures were characterized.
32254789	5	85	used	used	912:915	arg2	FITC-BSA					898:905	FITC-BSA	898:905	FITC-BSA	898:905	Fluorescein isothiocyanate-conjugated bovine serum albumin (FITC-BSA) was used as a model protein and the developed structures were characterized.
32254789	4	86	dep	poly-d	754:759	arg1	l-lactic-co-glycolic					761:780	l-lactic-co-glycolic	761:780	l-lactic-co-glycolic	761:780	Here we report a two-phase PLGA/alginate delivery system composed of protein-laden poly-d,l-lactic-co-glycolic acid (PLGA) microspheres wet-spun into alginate fibres.
32254789	9	87	with	scaffolds	1600:1608	arg1	ability					1619:1625	the ability to guide tissue regeneration through tightly-controlled release of one or more proteins in highly defined spatio-temporal configurations	1615:1762	the ability to guide tissue regeneration through tightly-controlled release of one or more proteins in highly defined spatio-temporal configurations	1615:1762	The "programmable" microsphere concentration gradient fibre methodology presented here may enable development of novel alginate scaffolds with the ability to guide tissue regeneration through tightly-controlled release of one or more proteins in highly defined spatio-temporal configurations.
32254789	3	88	theme	multiple	577:584	arg1	molecules					597:605	(multiple) bioactive molecules	576:605	(multiple) bioactive molecules delivered at a concentration gradient for chemotactic purposes	576:668	This largely limits alginate-mediated bioactive molecule delivery to single-factors of uniform concentration applications, rather than applications that may require (multiple) bioactive molecules delivered at a concentration gradient for chemotactic purposes.
30557272	10	0	from	%	1369:1369	arg1	groups					1416:1421	the EJ and EJ-J groups	1400:1421	groups	1416:1421	After islet transplantation into streptozotocin-induced diabetic mice, blood glucose levels reached the normoglycemic range in 61.5% and 7.7% of diabetic mice in the EJ and EJ-J groups, respectively.
30557272	10	1	theme	normoglycemic	1342:1354	arg1	mice					1392:1395	diabetic mice	1383:1395	diabetic mice	1383:1395	After islet transplantation into streptozotocin-induced diabetic mice, blood glucose levels reached the normoglycemic range in 61.5% and 7.7% of diabetic mice in the EJ and EJ-J groups, respectively.
30557272	10	1	theme	normoglycemic	1342:1354	arg1	%					1369:1369	the normoglycemic range in 61.5%	1338:1369	the normoglycemic range in 61.5%	1338:1369	After islet transplantation into streptozotocin-induced diabetic mice, blood glucose levels reached the normoglycemic range in 61.5% and 7.7% of diabetic mice in the EJ and EJ-J groups, respectively.
30557272	8	2	theme	low	1097:1099	arg1	level					1101:1105	a relatively low level	1084:1105	a relatively low level	1084:1105	After pancreas preservation in EJ solution, JNK activity was maintained at a relatively low level during islet isolation.
30557272	1	3	with	yield	319:323	arg1	viability					346:354	poor viability	341:354	poor viability	341:354	BACKGROUND For islet transplantation, pancreas preservation in University of Wisconsin (UW) solution is associated with disadvantages, such as collagenase inhibition, resulting in poor islet yield and islets with poor viability.
30557272	6	4	theme	Islet	785:789	arg1	yield					791:795	Islet yield	785:795	Islet yield before and after purification	785:825	Islet yield before and after purification was significantly higher in the EJ group than in the UW group.
30557272	13	5	theme	UW	1732:1733	arg1	solution					1735:1742	UW solution	1732:1742	UW solution	1732:1742	EJ solution may therefore be more suitable for islet isolation than UW solution.
30557272	3	6	theme	EJ	517:518	arg1	solution					520:527	The EJ solution	513:527	The EJ solution	513:527	METHODS The EJ solution has high sodium-low potassium composition with low viscosity compared to UW solution.
30557272	6	7	theme	EJ	859:860	arg1	group					862:866	the EJ group	855:866	the EJ group	855:866	Islet yield before and after purification was significantly higher in the EJ group than in the UW group.
30557272	4	8	theme	developed	657:665	arg1	inhibitor					671:679	a recently developed JNK inhibitor	646:679	a recently developed JNK inhibitor from our laboratory	646:699	Moreover, EJ solution contains a recently developed JNK inhibitor from our laboratory.
30557272	10	9	theme	in	1362:1363	arg1	mice					1392:1395	diabetic mice	1383:1395	diabetic mice	1383:1395	After islet transplantation into streptozotocin-induced diabetic mice, blood glucose levels reached the normoglycemic range in 61.5% and 7.7% of diabetic mice in the EJ and EJ-J groups, respectively.
30557272	10	9	theme	in	1362:1363	arg1	%					1369:1369	the normoglycemic range in 61.5%	1338:1369	the normoglycemic range in 61.5%	1338:1369	After islet transplantation into streptozotocin-induced diabetic mice, blood glucose levels reached the normoglycemic range in 61.5% and 7.7% of diabetic mice in the EJ and EJ-J groups, respectively.
30557272	3	10	theme	sodium-low	538:547	arg1	composition					559:569	high sodium-low potassium composition	533:569	high sodium-low potassium composition	533:569	METHODS The EJ solution has high sodium-low potassium composition with low viscosity compared to UW solution.
30557272	12	11	theme	CONCLUSIONS	1541:1551	arg1	Advantages					1553:1562	CONCLUSIONS Advantages	1541:1562	CONCLUSIONS Advantages of EJ solution over UW solution	1541:1594	CONCLUSIONS Advantages of EJ solution over UW solution were inhibition of JNK activity and reduced collagenase inhibition.
30557272	7	12	theme	EJ-J	993:996	arg1	inhibitor					982:990	the JNK inhibitor	974:990	the JNK inhibitor (EJ-J solution)	974:1006	Second, we compared the performance of EJ solution with that of EJ solution without the JNK inhibitor (EJ-J solution).
30557272	7	12	theme	EJ-J	993:996	arg1	solution					998:1005	EJ-J solution	993:1005	EJ-J solution	993:1005	Second, we compared the performance of EJ solution with that of EJ solution without the JNK inhibitor (EJ-J solution).
30557272	3	13	theme	low	576:578	arg1	viscosity					580:588	low viscosity	576:588	low viscosity compared to UW solution	576:612	METHODS The EJ solution has high sodium-low potassium composition with low viscosity compared to UW solution.
30557272	7	14	theme	EJ	954:955	arg1	solution					957:964	EJ solution	954:964	EJ solution without the JNK inhibitor (EJ-J solution)	954:1006	Second, we compared the performance of EJ solution with that of EJ solution without the JNK inhibitor (EJ-J solution).
30557272	12	15	theme	collagenase	1640:1650	arg1	inhibition					1652:1661	reduced collagenase inhibition	1632:1661	reduced collagenase inhibition	1632:1661	CONCLUSIONS Advantages of EJ solution over UW solution were inhibition of JNK activity and reduced collagenase inhibition.
30557272	9	16	theme	EJ	1205:1206	arg1	group					1208:1212	the EJ group	1201:1212	the EJ group	1201:1212	Islet yield before and after purification was significantly higher in the EJ group than in the EJ-J group.
30557272	5	17	dep	RESULTS	702:708	arg1	compared					719:726	compared	719:726	compared the performance of EJ solution with that of UW solution	719:782	RESULTS We first compared the performance of EJ solution with that of UW solution.
30557272	5	18	theme	EJ	747:748	arg1	solution					750:757	EJ solution	747:757	EJ solution	747:757	RESULTS We first compared the performance of EJ solution with that of UW solution.
30557272	3	19	theme	UW	602:603	arg1	solution					605:612	UW solution	602:612	UW solution	602:612	METHODS The EJ solution has high sodium-low potassium composition with low viscosity compared to UW solution.
30557272	7	20	theme	solution	932:939	arg1	performance					914:924	the performance	910:924	the performance of EJ solution	910:939	Second, we compared the performance of EJ solution with that of EJ solution without the JNK inhibitor (EJ-J solution).
30557272	12	21	theme	JNK	1615:1617	arg1	activity					1619:1626	JNK activity	1615:1626	JNK activity	1615:1626	CONCLUSIONS Advantages of EJ solution over UW solution were inhibition of JNK activity and reduced collagenase inhibition.
30557272	2	22	theme	inhibitor-containing	469:488	arg1	solution					495:502	the extracellular-type c-Jun N-terminal kinase (JNK) inhibitor-containing (EJ) solution	416:502	the extracellular-type c-Jun N-terminal kinase (JNK) inhibitor-containing (EJ) solution	416:502	In this study, we evaluated a novel preservation solution, the extracellular-type c-Jun N-terminal kinase (JNK) inhibitor-containing (EJ) solution.
30557272	2	22	theme	inhibitor-containing	469:488	arg1	solution					406:413	a novel preservation solution	385:413	a novel preservation solution	385:413	In this study, we evaluated a novel preservation solution, the extracellular-type c-Jun N-terminal kinase (JNK) inhibitor-containing (EJ) solution.
30557272	1	23	theme	pancreas	166:173	arg1	transplantation					149:163	islet transplantation	143:163	islet transplantation	143:163	BACKGROUND For islet transplantation, pancreas preservation in University of Wisconsin (UW) solution is associated with disadvantages, such as collagenase inhibition, resulting in poor islet yield and islets with poor viability.
30557272	1	23	theme	pancreas	166:173	arg1	preservation					175:186	pancreas preservation	166:186	pancreas preservation	166:186	BACKGROUND For islet transplantation, pancreas preservation in University of Wisconsin (UW) solution is associated with disadvantages, such as collagenase inhibition, resulting in poor islet yield and islets with poor viability.
30557272	0	24	theme	Porcine	103:109	arg1	Isolation					117:125	Porcine Islet Isolation	103:125	Porcine Islet Isolation	103:125	A Novel Preservation Solution Containing a JNK Inhibitory Peptide Efficiently Improves Islet Yield for Porcine Islet Isolation.
30557272	1	25	from	BACKGROUND	128:137	arg1	University					191:200	University	191:200	University	191:200	BACKGROUND For islet transplantation, pancreas preservation in University of Wisconsin (UW) solution is associated with disadvantages, such as collagenase inhibition, resulting in poor islet yield and islets with poor viability.
30557272	2	26	theme	c-Jun	439:443	arg1	kinase					456:461	the extracellular-type c-Jun N-terminal kinase	416:461	the extracellular-type c-Jun N-terminal kinase (JNK) inhibitor-containing (EJ) solution	416:502	In this study, we evaluated a novel preservation solution, the extracellular-type c-Jun N-terminal kinase (JNK) inhibitor-containing (EJ) solution.
30557272	10	27	theme	mice	1392:1395	arg1	%					1378:1378	7.7%	1375:1378	7.7% of diabetic mice in the EJ and EJ-J groups	1375:1421	After islet transplantation into streptozotocin-induced diabetic mice, blood glucose levels reached the normoglycemic range in 61.5% and 7.7% of diabetic mice in the EJ and EJ-J groups, respectively.
30557272	10	27	theme	mice	1392:1395	arg1	mice					1392:1395	diabetic mice	1383:1395	diabetic mice	1383:1395	After islet transplantation into streptozotocin-induced diabetic mice, blood glucose levels reached the normoglycemic range in 61.5% and 7.7% of diabetic mice in the EJ and EJ-J groups, respectively.
30557272	10	27	theme	mice	1392:1395	arg1	%					1369:1369	the normoglycemic range in 61.5%	1338:1369	the normoglycemic range in 61.5%	1338:1369	After islet transplantation into streptozotocin-induced diabetic mice, blood glucose levels reached the normoglycemic range in 61.5% and 7.7% of diabetic mice in the EJ and EJ-J groups, respectively.
30557272	2	28	theme	EJ	491:492	arg1	solution					495:502	the extracellular-type c-Jun N-terminal kinase (JNK) inhibitor-containing (EJ) solution	416:502	the extracellular-type c-Jun N-terminal kinase (JNK) inhibitor-containing (EJ) solution	416:502	In this study, we evaluated a novel preservation solution, the extracellular-type c-Jun N-terminal kinase (JNK) inhibitor-containing (EJ) solution.
30557272	2	28	theme	EJ	491:492	arg1	solution					406:413	a novel preservation solution	385:413	a novel preservation solution	385:413	In this study, we evaluated a novel preservation solution, the extracellular-type c-Jun N-terminal kinase (JNK) inhibitor-containing (EJ) solution.
30557272	1	29	theme	collagenase	271:281	arg1	inhibition					283:292	collagenase inhibition	271:292	collagenase inhibition	271:292	BACKGROUND For islet transplantation, pancreas preservation in University of Wisconsin (UW) solution is associated with disadvantages, such as collagenase inhibition, resulting in poor islet yield and islets with poor viability.
30557272	12	30	theme	EJ	1567:1568	arg1	solution					1570:1577	EJ solution	1567:1577	EJ solution	1567:1577	CONCLUSIONS Advantages of EJ solution over UW solution were inhibition of JNK activity and reduced collagenase inhibition.
30557272	11	31	theme	EJ	1448:1449	arg1	solution					1451:1458	EJ solution	1448:1458	EJ solution	1448:1458	Moreover, EJ solution exhibited reduced inhibition of collagenase digestion compared with UW solution.
30557272	0	32	theme	Preservation	8:19	arg1	Solution					21:28	A Novel Preservation Solution	0:28	A Novel Preservation Solution Containing a JNK Inhibitory Peptide	0:64	A Novel Preservation Solution Containing a JNK Inhibitory Peptide Efficiently Improves Islet Yield for Porcine Islet Isolation.
30557272	8	33	theme	pancreas	1015:1022	arg1	preservation					1024:1035	pancreas preservation	1015:1035	pancreas preservation in EJ solution	1015:1050	After pancreas preservation in EJ solution, JNK activity was maintained at a relatively low level during islet isolation.
30557272	3	34	dep	METHODS	505:511	arg1	has					529:531	has	529:531	has high sodium-low potassium composition with low viscosity compared to UW solution	529:612	METHODS The EJ solution has high sodium-low potassium composition with low viscosity compared to UW solution.
30557272	2	35	theme	JNK	464:466	arg1	solution					495:502	the extracellular-type c-Jun N-terminal kinase (JNK) inhibitor-containing (EJ) solution	416:502	the extracellular-type c-Jun N-terminal kinase (JNK) inhibitor-containing (EJ) solution	416:502	In this study, we evaluated a novel preservation solution, the extracellular-type c-Jun N-terminal kinase (JNK) inhibitor-containing (EJ) solution.
30557272	2	35	theme	JNK	464:466	arg1	solution					406:413	a novel preservation solution	385:413	a novel preservation solution	385:413	In this study, we evaluated a novel preservation solution, the extracellular-type c-Jun N-terminal kinase (JNK) inhibitor-containing (EJ) solution.
30557272	2	36	theme	novel	387:391	arg1	solution					495:502	the extracellular-type c-Jun N-terminal kinase (JNK) inhibitor-containing (EJ) solution	416:502	the extracellular-type c-Jun N-terminal kinase (JNK) inhibitor-containing (EJ) solution	416:502	In this study, we evaluated a novel preservation solution, the extracellular-type c-Jun N-terminal kinase (JNK) inhibitor-containing (EJ) solution.
30557272	2	36	theme	novel	387:391	arg1	solution					406:413	a novel preservation solution	385:413	a novel preservation solution	385:413	In this study, we evaluated a novel preservation solution, the extracellular-type c-Jun N-terminal kinase (JNK) inhibitor-containing (EJ) solution.
30557272	2	37	theme	kinase	456:461	arg1	solution					495:502	the extracellular-type c-Jun N-terminal kinase (JNK) inhibitor-containing (EJ) solution	416:502	the extracellular-type c-Jun N-terminal kinase (JNK) inhibitor-containing (EJ) solution	416:502	In this study, we evaluated a novel preservation solution, the extracellular-type c-Jun N-terminal kinase (JNK) inhibitor-containing (EJ) solution.
30557272	2	37	theme	kinase	456:461	arg1	solution					406:413	a novel preservation solution	385:413	a novel preservation solution	385:413	In this study, we evaluated a novel preservation solution, the extracellular-type c-Jun N-terminal kinase (JNK) inhibitor-containing (EJ) solution.
30557272	11	38	theme	UW	1528:1529	arg1	solution					1531:1538	UW solution	1528:1538	UW solution	1528:1538	Moreover, EJ solution exhibited reduced inhibition of collagenase digestion compared with UW solution.
30557272	0	39	theme	JNK	43:45	arg1	Peptide					58:64	a JNK Inhibitory Peptide	41:64	a JNK Inhibitory Peptide	41:64	A Novel Preservation Solution Containing a JNK Inhibitory Peptide Efficiently Improves Islet Yield for Porcine Islet Isolation.
30557272	1	40	theme	islet	313:317	arg1	yield					319:323	poor islet yield	308:323	poor islet yield	308:323	BACKGROUND For islet transplantation, pancreas preservation in University of Wisconsin (UW) solution is associated with disadvantages, such as collagenase inhibition, resulting in poor islet yield and islets with poor viability.
30557272	8	41	theme	JNK	1053:1055	arg1	activity					1057:1064	JNK activity	1053:1064	JNK activity	1053:1064	After pancreas preservation in EJ solution, JNK activity was maintained at a relatively low level during islet isolation.
30557272	10	42	theme	blood	1309:1313	arg1	levels					1323:1328	blood glucose levels	1309:1328	blood glucose levels	1309:1328	After islet transplantation into streptozotocin-induced diabetic mice, blood glucose levels reached the normoglycemic range in 61.5% and 7.7% of diabetic mice in the EJ and EJ-J groups, respectively.
30557272	4	43	from	laboratory	690:699	arg1	inhibitor					671:679	a recently developed JNK inhibitor	646:679	a recently developed JNK inhibitor from our laboratory	646:699	Moreover, EJ solution contains a recently developed JNK inhibitor from our laboratory.
30557272	0	44	theme	Inhibitory	47:56	arg1	Peptide					58:64	a JNK Inhibitory Peptide	41:64	a JNK Inhibitory Peptide	41:64	A Novel Preservation Solution Containing a JNK Inhibitory Peptide Efficiently Improves Islet Yield for Porcine Islet Isolation.
30557272	5	45	theme	solution	750:757	arg1	performance					732:742	the performance	728:742	the performance of EJ solution	728:757	RESULTS We first compared the performance of EJ solution with that of UW solution.
30557272	6	46	theme	UW	880:881	arg1	group					883:887	the UW group	876:887	the UW group	876:887	Islet yield before and after purification was significantly higher in the EJ group than in the UW group.
30557272	13	47	theme	islet	1711:1715	arg1	isolation					1717:1725	islet isolation	1711:1725	islet isolation	1711:1725	EJ solution may therefore be more suitable for islet isolation than UW solution.
30557272	10	48	theme	streptozotocin-induced	1271:1292	arg1	mice					1303:1306	streptozotocin-induced diabetic mice	1271:1306	streptozotocin-induced diabetic mice	1271:1306	After islet transplantation into streptozotocin-induced diabetic mice, blood glucose levels reached the normoglycemic range in 61.5% and 7.7% of diabetic mice in the EJ and EJ-J groups, respectively.
30557272	9	49	theme	Islet	1131:1135	arg1	yield					1137:1141	Islet yield	1131:1141	Islet yield before and after purification	1131:1171	Islet yield before and after purification was significantly higher in the EJ group than in the EJ-J group.
30557272	11	50	theme	collagenase	1492:1502	arg1	digestion					1504:1512	collagenase digestion	1492:1512	collagenase digestion	1492:1512	Moreover, EJ solution exhibited reduced inhibition of collagenase digestion compared with UW solution.
30557272	3	51	theme	potassium	549:557	arg1	composition					559:569	high sodium-low potassium composition	533:569	high sodium-low potassium composition	533:569	METHODS The EJ solution has high sodium-low potassium composition with low viscosity compared to UW solution.
30557272	8	52	theme	islet	1114:1118	arg1	isolation					1120:1128	islet isolation	1114:1128	islet isolation	1114:1128	After pancreas preservation in EJ solution, JNK activity was maintained at a relatively low level during islet isolation.
30557272	11	53	theme	reduced	1470:1476	arg1	inhibition					1478:1487	reduced inhibition	1470:1487	reduced inhibition of collagenase digestion	1470:1512	Moreover, EJ solution exhibited reduced inhibition of collagenase digestion compared with UW solution.
30557272	10	54	theme	range	1356:1360	arg1	mice					1392:1395	diabetic mice	1383:1395	diabetic mice	1383:1395	After islet transplantation into streptozotocin-induced diabetic mice, blood glucose levels reached the normoglycemic range in 61.5% and 7.7% of diabetic mice in the EJ and EJ-J groups, respectively.
30557272	10	54	theme	range	1356:1360	arg1	%					1369:1369	the normoglycemic range in 61.5%	1338:1369	the normoglycemic range in 61.5%	1338:1369	After islet transplantation into streptozotocin-induced diabetic mice, blood glucose levels reached the normoglycemic range in 61.5% and 7.7% of diabetic mice in the EJ and EJ-J groups, respectively.
30557272	3	55	theme	high	533:536	arg1	composition					559:569	high sodium-low potassium composition	533:569	high sodium-low potassium composition	533:569	METHODS The EJ solution has high sodium-low potassium composition with low viscosity compared to UW solution.
30557272	1	56	theme	Wisconsin	205:213	arg1	solution					220:227	Wisconsin (UW) solution	205:227	Wisconsin (UW) solution	205:227	BACKGROUND For islet transplantation, pancreas preservation in University of Wisconsin (UW) solution is associated with disadvantages, such as collagenase inhibition, resulting in poor islet yield and islets with poor viability.
30557272	13	57	theme	EJ	1664:1665	arg1	solution					1667:1674	EJ solution	1664:1674	EJ solution	1664:1674	EJ solution may therefore be more suitable for islet isolation than UW solution.
30557272	1	58	theme	UW	216:217	arg1	solution					220:227	Wisconsin (UW) solution	205:227	Wisconsin (UW) solution	205:227	BACKGROUND For islet transplantation, pancreas preservation in University of Wisconsin (UW) solution is associated with disadvantages, such as collagenase inhibition, resulting in poor islet yield and islets with poor viability.
30557272	4	59	theme	EJ	625:626	arg1	solution					628:635	EJ solution	625:635	EJ solution	625:635	Moreover, EJ solution contains a recently developed JNK inhibitor from our laboratory.
30557272	9	60	theme	EJ-J	1226:1229	arg1	group					1231:1235	the EJ-J group	1222:1235	the EJ-J group	1222:1235	Islet yield before and after purification was significantly higher in the EJ group than in the EJ-J group.
30557272	7	61	theme	JNK	978:980	arg1	inhibitor					982:990	the JNK inhibitor	974:990	the JNK inhibitor (EJ-J solution)	974:1006	Second, we compared the performance of EJ solution with that of EJ solution without the JNK inhibitor (EJ-J solution).
30557272	7	61	theme	JNK	978:980	arg1	solution					998:1005	EJ-J solution	993:1005	EJ-J solution	993:1005	Second, we compared the performance of EJ solution with that of EJ solution without the JNK inhibitor (EJ-J solution).
30557272	12	62	theme	reduced	1632:1638	arg1	inhibition					1652:1661	reduced collagenase inhibition	1632:1661	reduced collagenase inhibition	1632:1661	CONCLUSIONS Advantages of EJ solution over UW solution were inhibition of JNK activity and reduced collagenase inhibition.
30557272	12	63	theme	activity	1619:1626	arg1	inhibition					1652:1661	reduced collagenase inhibition	1632:1661	reduced collagenase inhibition	1632:1661	CONCLUSIONS Advantages of EJ solution over UW solution were inhibition of JNK activity and reduced collagenase inhibition.
30557272	12	63	theme	activity	1619:1626	arg1	inhibition					1601:1610	inhibition	1601:1610	inhibition of JNK activity	1601:1626	CONCLUSIONS Advantages of EJ solution over UW solution were inhibition of JNK activity and reduced collagenase inhibition.
30557272	1	64	theme	islet	143:147	arg1	transplantation					149:163	islet transplantation	143:163	islet transplantation	143:163	BACKGROUND For islet transplantation, pancreas preservation in University of Wisconsin (UW) solution is associated with disadvantages, such as collagenase inhibition, resulting in poor islet yield and islets with poor viability.
30557272	1	64	theme	islet	143:147	arg1	preservation					175:186	pancreas preservation	166:186	pancreas preservation	166:186	BACKGROUND For islet transplantation, pancreas preservation in University of Wisconsin (UW) solution is associated with disadvantages, such as collagenase inhibition, resulting in poor islet yield and islets with poor viability.
30557272	8	65	from	preservation	1024:1035	arg1	solution					1043:1050	EJ solution	1040:1050	EJ solution	1040:1050	After pancreas preservation in EJ solution, JNK activity was maintained at a relatively low level during islet isolation.
30557272	4	66	theme	JNK	667:669	arg1	inhibitor					671:679	a recently developed JNK inhibitor	646:679	a recently developed JNK inhibitor from our laboratory	646:699	Moreover, EJ solution contains a recently developed JNK inhibitor from our laboratory.
30557272	0	67	theme	Islet	87:91	arg1	Yield					93:97	Islet Yield	87:97	Islet Yield for Porcine Islet Isolation	87:125	A Novel Preservation Solution Containing a JNK Inhibitory Peptide Efficiently Improves Islet Yield for Porcine Islet Isolation.
30557272	1	68	theme	poor	341:344	arg1	viability					346:354	poor viability	341:354	poor viability	341:354	BACKGROUND For islet transplantation, pancreas preservation in University of Wisconsin (UW) solution is associated with disadvantages, such as collagenase inhibition, resulting in poor islet yield and islets with poor viability.
30557272	7	69	theme	EJ	929:930	arg1	solution					932:939	EJ solution	929:939	EJ solution	929:939	Second, we compared the performance of EJ solution with that of EJ solution without the JNK inhibitor (EJ-J solution).
30557272	1	70	with	islets	329:334	arg1	viability					346:354	poor viability	341:354	poor viability	341:354	BACKGROUND For islet transplantation, pancreas preservation in University of Wisconsin (UW) solution is associated with disadvantages, such as collagenase inhibition, resulting in poor islet yield and islets with poor viability.
30557272	0	71	theme	Islet	111:115	arg1	Isolation					117:125	Porcine Islet Isolation	103:125	Porcine Islet Isolation	103:125	A Novel Preservation Solution Containing a JNK Inhibitory Peptide Efficiently Improves Islet Yield for Porcine Islet Isolation.
30557272	12	72	theme	UW	1584:1585	arg1	solution					1587:1594	UW solution	1584:1594	UW solution	1584:1594	CONCLUSIONS Advantages of EJ solution over UW solution were inhibition of JNK activity and reduced collagenase inhibition.
30557272	0	73	theme	Novel	2:6	arg1	Solution					21:28	A Novel Preservation Solution	0:28	A Novel Preservation Solution Containing a JNK Inhibitory Peptide	0:64	A Novel Preservation Solution Containing a JNK Inhibitory Peptide Efficiently Improves Islet Yield for Porcine Islet Isolation.
30557272	10	74	from	%	1378:1378	arg1	groups					1416:1421	the EJ and EJ-J groups	1400:1421	groups	1416:1421	After islet transplantation into streptozotocin-induced diabetic mice, blood glucose levels reached the normoglycemic range in 61.5% and 7.7% of diabetic mice in the EJ and EJ-J groups, respectively.
30557272	2	75	theme	extracellular-type	420:437	arg1	kinase					456:461	the extracellular-type c-Jun N-terminal kinase	416:461	the extracellular-type c-Jun N-terminal kinase (JNK) inhibitor-containing (EJ) solution	416:502	In this study, we evaluated a novel preservation solution, the extracellular-type c-Jun N-terminal kinase (JNK) inhibitor-containing (EJ) solution.
30557272	10	76	theme	diabetic	1383:1390	arg1	mice					1392:1395	diabetic mice	1383:1395	diabetic mice	1383:1395	After islet transplantation into streptozotocin-induced diabetic mice, blood glucose levels reached the normoglycemic range in 61.5% and 7.7% of diabetic mice in the EJ and EJ-J groups, respectively.
30557272	10	77	theme	islet	1244:1248	arg1	transplantation					1250:1264	islet transplantation	1244:1264	islet transplantation into streptozotocin-induced diabetic mice	1244:1306	After islet transplantation into streptozotocin-induced diabetic mice, blood glucose levels reached the normoglycemic range in 61.5% and 7.7% of diabetic mice in the EJ and EJ-J groups, respectively.
30557272	10	78	theme	EJ-J	1411:1414	arg1	groups					1416:1421	the EJ and EJ-J groups	1400:1421	groups	1416:1421	After islet transplantation into streptozotocin-induced diabetic mice, blood glucose levels reached the normoglycemic range in 61.5% and 7.7% of diabetic mice in the EJ and EJ-J groups, respectively.
30557272	2	79	theme	preservation	393:404	arg1	solution					495:502	the extracellular-type c-Jun N-terminal kinase (JNK) inhibitor-containing (EJ) solution	416:502	the extracellular-type c-Jun N-terminal kinase (JNK) inhibitor-containing (EJ) solution	416:502	In this study, we evaluated a novel preservation solution, the extracellular-type c-Jun N-terminal kinase (JNK) inhibitor-containing (EJ) solution.
30557272	2	79	theme	preservation	393:404	arg1	solution					406:413	a novel preservation solution	385:413	a novel preservation solution	385:413	In this study, we evaluated a novel preservation solution, the extracellular-type c-Jun N-terminal kinase (JNK) inhibitor-containing (EJ) solution.
30557272	8	80	theme	EJ	1040:1041	arg1	solution					1043:1050	EJ solution	1040:1050	EJ solution	1040:1050	After pancreas preservation in EJ solution, JNK activity was maintained at a relatively low level during islet isolation.
30557272	12	81	theme	solution	1570:1577	arg1	Advantages					1553:1562	CONCLUSIONS Advantages	1541:1562	CONCLUSIONS Advantages of EJ solution over UW solution	1541:1594	CONCLUSIONS Advantages of EJ solution over UW solution were inhibition of JNK activity and reduced collagenase inhibition.
30557272	10	82	theme	EJ	1404:1405	arg1	groups					1416:1421	the EJ and EJ-J groups	1400:1421	groups	1416:1421	After islet transplantation into streptozotocin-induced diabetic mice, blood glucose levels reached the normoglycemic range in 61.5% and 7.7% of diabetic mice in the EJ and EJ-J groups, respectively.
30557272	10	83	theme	diabetic	1294:1301	arg1	mice					1303:1306	streptozotocin-induced diabetic mice	1271:1306	streptozotocin-induced diabetic mice	1271:1306	After islet transplantation into streptozotocin-induced diabetic mice, blood glucose levels reached the normoglycemic range in 61.5% and 7.7% of diabetic mice in the EJ and EJ-J groups, respectively.
30557272	5	84	theme	UW	772:773	arg1	solution					775:782	UW solution	772:782	UW solution	772:782	RESULTS We first compared the performance of EJ solution with that of UW solution.
30557272	1	85	theme	poor	308:311	arg1	yield					319:323	poor islet yield	308:323	poor islet yield	308:323	BACKGROUND For islet transplantation, pancreas preservation in University of Wisconsin (UW) solution is associated with disadvantages, such as collagenase inhibition, resulting in poor islet yield and islets with poor viability.
30557272	2	86	theme	N-terminal	445:454	arg1	kinase					456:461	the extracellular-type c-Jun N-terminal kinase	416:461	the extracellular-type c-Jun N-terminal kinase (JNK) inhibitor-containing (EJ) solution	416:502	In this study, we evaluated a novel preservation solution, the extracellular-type c-Jun N-terminal kinase (JNK) inhibitor-containing (EJ) solution.
30557272	3	87	contain	has	529:531	arg1	solution					520:527	The EJ solution	513:527	The EJ solution	513:527	METHODS The EJ solution has high sodium-low potassium composition with low viscosity compared to UW solution.
30557272	3	87	contain	has	529:531	arg2	composition					559:569	high sodium-low potassium composition	533:569	high sodium-low potassium composition	533:569	METHODS The EJ solution has high sodium-low potassium composition with low viscosity compared to UW solution.
30557272	11	88	theme	digestion	1504:1512	arg1	inhibition					1478:1487	reduced inhibition	1470:1487	reduced inhibition of collagenase digestion	1470:1512	Moreover, EJ solution exhibited reduced inhibition of collagenase digestion compared with UW solution.
30557272	4	89	contain	contains	637:644	arg1	solution					628:635	EJ solution	625:635	EJ solution	625:635	Moreover, EJ solution contains a recently developed JNK inhibitor from our laboratory.
30557272	4	89	contain	contains	637:644	arg2	inhibitor					671:679	a recently developed JNK inhibitor	646:679	a recently developed JNK inhibitor from our laboratory	646:699	Moreover, EJ solution contains a recently developed JNK inhibitor from our laboratory.
30557272	10	90	theme	glucose	1315:1321	arg1	levels					1323:1328	blood glucose levels	1309:1328	blood glucose levels	1309:1328	After islet transplantation into streptozotocin-induced diabetic mice, blood glucose levels reached the normoglycemic range in 61.5% and 7.7% of diabetic mice in the EJ and EJ-J groups, respectively.
31085703	11	0	theme	difficile	1349:1357	arg1	proteins					1359:1366	C. difficile proteins	1346:1366	C. difficile proteins associated with the cell wall	1346:1396	A proteomic analysis showed that the majority of C. difficile proteins associated with the cell wall were less abundant in the ΔprkC mutant than the wild-type strain.
31085703	4	1	theme	PASTA	506:510	arg1	domains					512:518	two PASTA domains	502:518	two PASTA domains	502:518	PrkC of C. difficile is an STK with two PASTA domains.
31085703	8	2	theme	secondary	976:984	arg1	deoxycholate					996:1007	the secondary bile salt deoxycholate	972:1007	the secondary bile salt deoxycholate	972:1007	Moreover, a ΔprkC mutant was more sensitive to antimicrobial compounds that target the cell envelope, such as the secondary bile salt deoxycholate, cephalosporins, cationic antimicrobial peptides, and lysozyme.
31085703	7	3	dep	able	759:762	arg1	sporulate					767:775	sporulate	767:775	sporulate	767:775	A ΔprkC mutant was able to sporulate and germinate but was less motile and formed more biofilm than the wild-type strain.
31085703	7	3	dep	able	759:762	arg1	motile					804:809	motile	804:809	motile	804:809	A ΔprkC mutant was able to sporulate and germinate but was less motile and formed more biofilm than the wild-type strain.
31085703	7	3	dep	able	759:762	arg1	mutant					748:753	A ΔprkC mutant	740:753	A ΔprkC mutant	740:753	A ΔprkC mutant was able to sporulate and germinate but was less motile and formed more biofilm than the wild-type strain.
31085703	7	3	dep	able	759:762	arg1	able					759:762	able	759:762	able	759:762	A ΔprkC mutant was able to sporulate and germinate but was less motile and formed more biofilm than the wild-type strain.
31085703	7	3	dep	able	759:762	arg1	germinate					781:789	germinate	781:789	germinate	781:789	A ΔprkC mutant was able to sporulate and germinate but was less motile and formed more biofilm than the wild-type strain.
31085703	4	4	with	STK	493:495	arg1	domains					512:518	two PASTA domains	502:518	two PASTA domains	502:518	PrkC of C. difficile is an STK with two PASTA domains.
31085703	6	5	theme	prkC	621:624	arg1	deletion					609:616	deletion	609:616	deletion of prkC	609:624	We observed that deletion of prkC affects cell morphology with an increase in mean size, cell length heterogeneity, and presence of abnormal septa.
31085703	0	6	theme	Antimicrobial	66:78	arg1	Resistance					80:89	Antimicrobial Resistance	66:89	Antimicrobial Resistance	66:89	The Ser/Thr Kinase PrkC Participates in Cell Wall Homeostasis and Antimicrobial Resistance in Clostridium difficile.
31085703	1	7	theme	antibiotic-associated	163:183	arg1	diarrhea					185:192	antibiotic-associated diarrhea	163:192	antibiotic-associated diarrhea in adults	163:202	Clostridium difficile is the leading cause of antibiotic-associated diarrhea in adults.
31085703	0	8	theme	Clostridium	94:104	arg1	difficile					106:114	Clostridium difficile	94:114	Clostridium difficile	94:114	The Ser/Thr Kinase PrkC Participates in Cell Wall Homeostasis and Antimicrobial Resistance in Clostridium difficile.
31085703	12	9	contain	had	1523:1525	arg1	mutant					1516:1521	the ΔprkC mutant	1506:1521	the ΔprkC mutant	1506:1521	Finally, in a hamster model of infection, the ΔprkC mutant had a colonization delay that did not significantly affect overall virulence.
31085703	12	9	contain	had	1523:1525	arg2	delay					1542:1546	a colonization delay	1527:1546	a colonization delay that did not significantly affect overall virulence	1527:1598	Finally, in a hamster model of infection, the ΔprkC mutant had a colonization delay that did not significantly affect overall virulence.
31085703	8	10	theme	salt	991:994	arg1	deoxycholate					996:1007	the secondary bile salt deoxycholate	972:1007	the secondary bile salt deoxycholate	972:1007	Moreover, a ΔprkC mutant was more sensitive to antimicrobial compounds that target the cell envelope, such as the secondary bile salt deoxycholate, cephalosporins, cationic antimicrobial peptides, and lysozyme.
31085703	8	11	theme	antimicrobial	909:921	arg1	compounds					923:931	antimicrobial compounds	909:931	antimicrobial compounds that target the cell envelope	909:961	Moreover, a ΔprkC mutant was more sensitive to antimicrobial compounds that target the cell envelope, such as the secondary bile salt deoxycholate, cephalosporins, cationic antimicrobial peptides, and lysozyme.
31085703	3	12	theme	key	390:392	arg1	roles					394:398	key roles	390:398	key roles	390:398	Signal transduction networks involving serine/threonine kinases (STKs) play key roles in adaptation, as they regulate numerous physiological processes.
31085703	8	13	theme	bile	986:989	arg1	deoxycholate					996:1007	the secondary bile salt deoxycholate	972:1007	the secondary bile salt deoxycholate	972:1007	Moreover, a ΔprkC mutant was more sensitive to antimicrobial compounds that target the cell envelope, such as the secondary bile salt deoxycholate, cephalosporins, cationic antimicrobial peptides, and lysozyme.
31085703	8	14	theme	cell	949:952	arg1	envelope					954:961	the cell envelope	945:961	the cell envelope	945:961	Moreover, a ΔprkC mutant was more sensitive to antimicrobial compounds that target the cell envelope, such as the secondary bile salt deoxycholate, cephalosporins, cationic antimicrobial peptides, and lysozyme.
31085703	2	15	theme	survival	295:302	arg1	strategy					304:311	an appropriate survival strategy	280:311	an appropriate survival strategy	280:311	During infection, C. difficile must detect the host environment and induce an appropriate survival strategy.
31085703	12	16	theme	infection	1495:1503	arg1	model					1486:1490	a hamster model	1476:1490	a hamster model of infection	1476:1503	Finally, in a hamster model of infection, the ΔprkC mutant had a colonization delay that did not significantly affect overall virulence.
31085703	1	17	from	cause	154:158	arg1	adults					197:202	adults	197:202	adults	197:202	Clostridium difficile is the leading cause of antibiotic-associated diarrhea in adults.
31085703	2	18	theme	appropriate	283:293	arg1	strategy					304:311	an appropriate survival strategy	280:311	an appropriate survival strategy	280:311	During infection, C. difficile must detect the host environment and induce an appropriate survival strategy.
31085703	6	19	theme	length	686:691	arg1	heterogeneity					693:705	cell length heterogeneity	681:705	cell length heterogeneity	681:705	We observed that deletion of prkC affects cell morphology with an increase in mean size, cell length heterogeneity, and presence of abnormal septa.
31085703	1	20	theme	diarrhea	185:192	arg1	cause					154:158	the leading cause	142:158	the leading cause of antibiotic-associated diarrhea in adults	142:202	Clostridium difficile is the leading cause of antibiotic-associated diarrhea in adults.
31085703	1	20	theme	diarrhea	185:192	arg1	difficile					129:137	Clostridium difficile	117:137	Clostridium difficile	117:137	Clostridium difficile is the leading cause of antibiotic-associated diarrhea in adults.
31085703	11	21	theme	cell	1388:1391	arg1	wall					1393:1396	the cell wall	1384:1396	the cell wall	1384:1396	A proteomic analysis showed that the majority of C. difficile proteins associated with the cell wall were less abundant in the ΔprkC mutant than the wild-type strain.
31085703	6	22	theme	cell	681:684	arg1	heterogeneity					693:705	cell length heterogeneity	681:705	cell length heterogeneity	681:705	We observed that deletion of prkC affects cell morphology with an increase in mean size, cell length heterogeneity, and presence of abnormal septa.
31085703	0	23	theme	Ser/Thr	4:10	arg1	PrkC					19:22	The Ser/Thr Kinase PrkC	0:22	The Ser/Thr Kinase PrkC	0:22	The Ser/Thr Kinase PrkC Participates in Cell Wall Homeostasis and Antimicrobial Resistance in Clostridium difficile.
31085703	3	24	theme	serine/threonine	353:368	arg1	kinases					370:376	serine/threonine kinases	353:376	serine/threonine kinases (STKs)	353:383	Signal transduction networks involving serine/threonine kinases (STKs) play key roles in adaptation, as they regulate numerous physiological processes.
31085703	3	24	theme	serine/threonine	353:368	arg1	STKs					379:382	STKs	379:382	STKs	379:382	Signal transduction networks involving serine/threonine kinases (STKs) play key roles in adaptation, as they regulate numerous physiological processes.
31085703	9	25	from	differences	1127:1137	arg1	polysaccharide					1159:1172	polysaccharide	1159:1172	polysaccharide	1159:1172	This increased susceptibility was not associated with differences in peptidoglycan or polysaccharide II composition.
31085703	9	25	from	differences	1127:1137	arg1	peptidoglycan					1142:1154	peptidoglycan	1142:1154	peptidoglycan	1142:1154	This increased susceptibility was not associated with differences in peptidoglycan or polysaccharide II composition.
31085703	3	26	theme	physiological	441:453	arg1	processes					455:463	numerous physiological processes	432:463	numerous physiological processes	432:463	Signal transduction networks involving serine/threonine kinases (STKs) play key roles in adaptation, as they regulate numerous physiological processes.
31085703	6	27	from	increase	658:665	arg1	presence					712:719	presence	712:719	presence of abnormal septa	712:737	We observed that deletion of prkC affects cell morphology with an increase in mean size, cell length heterogeneity, and presence of abnormal septa.
31085703	6	27	from	increase	658:665	arg1	size					675:678	mean size	670:678	mean size	670:678	We observed that deletion of prkC affects cell morphology with an increase in mean size, cell length heterogeneity, and presence of abnormal septa.
31085703	6	27	from	increase	658:665	arg1	heterogeneity					693:705	cell length heterogeneity	681:705	cell length heterogeneity	681:705	We observed that deletion of prkC affects cell morphology with an increase in mean size, cell length heterogeneity, and presence of abnormal septa.
31085703	11	28	theme	proteomic	1299:1307	arg1	analysis					1309:1316	A proteomic analysis	1297:1316	A proteomic analysis	1297:1316	A proteomic analysis showed that the majority of C. difficile proteins associated with the cell wall were less abundant in the ΔprkC mutant than the wild-type strain.
31085703	8	29	theme	antimicrobial	1035:1047	arg1	peptides					1049:1056	cationic antimicrobial peptides	1026:1056	cationic antimicrobial peptides	1026:1056	Moreover, a ΔprkC mutant was more sensitive to antimicrobial compounds that target the cell envelope, such as the secondary bile salt deoxycholate, cephalosporins, cationic antimicrobial peptides, and lysozyme.
31085703	9	30	theme	II	1174:1175	arg1	composition					1177:1187	II composition	1174:1187	II composition	1174:1187	This increased susceptibility was not associated with differences in peptidoglycan or polysaccharide II composition.
31085703	6	31	theme	mean	670:673	arg1	size					675:678	mean size	670:678	mean size	670:678	We observed that deletion of prkC affects cell morphology with an increase in mean size, cell length heterogeneity, and presence of abnormal septa.
31085703	0	32	theme	Kinase	12:17	arg1	PrkC					19:22	The Ser/Thr Kinase PrkC	0:22	The Ser/Thr Kinase PrkC	0:22	The Ser/Thr Kinase PrkC Participates in Cell Wall Homeostasis and Antimicrobial Resistance in Clostridium difficile.
31085703	8	33	theme	cationic	1026:1033	arg1	peptides					1049:1056	cationic antimicrobial peptides	1026:1056	cationic antimicrobial peptides	1026:1056	Moreover, a ΔprkC mutant was more sensitive to antimicrobial compounds that target the cell envelope, such as the secondary bile salt deoxycholate, cephalosporins, cationic antimicrobial peptides, and lysozyme.
31085703	4	34	theme	C.	474:475	arg1	difficile					477:485	C. difficile	474:485	C. difficile	474:485	PrkC of C. difficile is an STK with two PASTA domains.
31085703	11	35	theme	ΔprkC	1424:1428	arg1	mutant					1430:1435	the ΔprkC mutant	1420:1435	the ΔprkC mutant	1420:1435	A proteomic analysis showed that the majority of C. difficile proteins associated with the cell wall were less abundant in the ΔprkC mutant than the wild-type strain.
31085703	10	36	theme	ΔprkC	1203:1207	arg1	mutant					1209:1214	the ΔprkC mutant	1199:1214	the ΔprkC mutant	1199:1214	However, the ΔprkC mutant had less peptidoglycan and released more polysaccharide II into the supernatant.
31085703	3	37	theme	numerous	432:439	arg1	processes					455:463	numerous physiological processes	432:463	numerous physiological processes	432:463	Signal transduction networks involving serine/threonine kinases (STKs) play key roles in adaptation, as they regulate numerous physiological processes.
31085703	12	38	theme	colonization	1529:1540	arg1	delay					1542:1546	a colonization delay	1527:1546	a colonization delay that did not significantly affect overall virulence	1527:1598	Finally, in a hamster model of infection, the ΔprkC mutant had a colonization delay that did not significantly affect overall virulence.
31085703	5	39	located	found	571:575	arg1	septum					584:589	the septum	580:589	the septum	580:589	We showed that PrkC is membrane associated and is found at the septum.
31085703	5	39	located	found	571:575	arg2	PrkC					536:539	PrkC	536:539	PrkC	536:539	We showed that PrkC is membrane associated and is found at the septum.
31085703	12	40	theme	hamster	1478:1484	arg1	model					1486:1490	a hamster model	1476:1490	a hamster model of infection	1476:1503	Finally, in a hamster model of infection, the ΔprkC mutant had a colonization delay that did not significantly affect overall virulence.
31085703	11	41	from	mutant	1430:1435	arg1	majority					1334:1341	the majority	1330:1341	the majority of C. difficile proteins associated with the cell wall	1330:1396	A proteomic analysis showed that the majority of C. difficile proteins associated with the cell wall were less abundant in the ΔprkC mutant than the wild-type strain.
31085703	11	41	from	mutant	1430:1435	arg1	abundant					1408:1415	abundant	1408:1415	abundant	1408:1415	A proteomic analysis showed that the majority of C. difficile proteins associated with the cell wall were less abundant in the ΔprkC mutant than the wild-type strain.
31085703	9	42	theme	increased	1078:1086	arg1	susceptibility					1088:1101	This increased susceptibility	1073:1101	This increased susceptibility	1073:1101	This increased susceptibility was not associated with differences in peptidoglycan or polysaccharide II composition.
31085703	0	43	theme	Wall	45:48	arg1	Homeostasis					50:60	Cell Wall Homeostasis	40:60	Cell Wall Homeostasis	40:60	The Ser/Thr Kinase PrkC Participates in Cell Wall Homeostasis and Antimicrobial Resistance in Clostridium difficile.
31085703	3	44	theme	transduction	321:332	arg1	networks					334:341	Signal transduction networks	314:341	Signal transduction networks involving serine/threonine kinases (STKs)	314:383	Signal transduction networks involving serine/threonine kinases (STKs) play key roles in adaptation, as they regulate numerous physiological processes.
31085703	4	45	theme	difficile	477:485	arg1	PrkC					466:469	PrkC	466:469	PrkC of C. difficile	466:485	PrkC of C. difficile is an STK with two PASTA domains.
31085703	4	45	theme	difficile	477:485	arg1	STK					493:495	an STK	490:495	an STK with two PASTA domains	490:518	PrkC of C. difficile is an STK with two PASTA domains.
31085703	8	46	theme	ΔprkC	874:878	arg1	mutant					880:885	a ΔprkC mutant	872:885	a ΔprkC mutant	872:885	Moreover, a ΔprkC mutant was more sensitive to antimicrobial compounds that target the cell envelope, such as the secondary bile salt deoxycholate, cephalosporins, cationic antimicrobial peptides, and lysozyme.
31085703	8	46	theme	ΔprkC	874:878	arg1	sensitive					896:904	sensitive	896:904	sensitive	896:904	Moreover, a ΔprkC mutant was more sensitive to antimicrobial compounds that target the cell envelope, such as the secondary bile salt deoxycholate, cephalosporins, cationic antimicrobial peptides, and lysozyme.
31085703	1	47	theme	Clostridium	117:127	arg1	cause					154:158	the leading cause	142:158	the leading cause of antibiotic-associated diarrhea in adults	142:202	Clostridium difficile is the leading cause of antibiotic-associated diarrhea in adults.
31085703	1	47	theme	Clostridium	117:127	arg1	difficile					129:137	Clostridium difficile	117:137	Clostridium difficile	117:137	Clostridium difficile is the leading cause of antibiotic-associated diarrhea in adults.
31085703	0	48	theme	Cell	40:43	arg1	Homeostasis					50:60	Cell Wall Homeostasis	40:60	Cell Wall Homeostasis	40:60	The Ser/Thr Kinase PrkC Participates in Cell Wall Homeostasis and Antimicrobial Resistance in Clostridium difficile.
31085703	7	49	theme	ΔprkC	742:746	arg1	sporulate					767:775	sporulate	767:775	sporulate	767:775	A ΔprkC mutant was able to sporulate and germinate but was less motile and formed more biofilm than the wild-type strain.
31085703	7	49	theme	ΔprkC	742:746	arg1	motile					804:809	motile	804:809	motile	804:809	A ΔprkC mutant was able to sporulate and germinate but was less motile and formed more biofilm than the wild-type strain.
31085703	7	49	theme	ΔprkC	742:746	arg1	able					759:762	able	759:762	able	759:762	A ΔprkC mutant was able to sporulate and germinate but was less motile and formed more biofilm than the wild-type strain.
31085703	7	49	theme	ΔprkC	742:746	arg1	mutant					748:753	A ΔprkC mutant	740:753	A ΔprkC mutant	740:753	A ΔprkC mutant was able to sporulate and germinate but was less motile and formed more biofilm than the wild-type strain.
31085703	7	49	theme	ΔprkC	742:746	arg1	germinate					781:789	germinate	781:789	germinate	781:789	A ΔprkC mutant was able to sporulate and germinate but was less motile and formed more biofilm than the wild-type strain.
31085703	2	50	theme	C.	223:224	arg1	difficile					226:234	C. difficile	223:234	C. difficile	223:234	During infection, C. difficile must detect the host environment and induce an appropriate survival strategy.
31085703	6	51	theme	septa	733:737	arg1	presence					712:719	presence	712:719	presence of abnormal septa	712:737	We observed that deletion of prkC affects cell morphology with an increase in mean size, cell length heterogeneity, and presence of abnormal septa.
31085703	6	51	theme	septa	733:737	arg1	size					675:678	mean size	670:678	mean size	670:678	We observed that deletion of prkC affects cell morphology with an increase in mean size, cell length heterogeneity, and presence of abnormal septa.
31085703	6	51	theme	septa	733:737	arg1	heterogeneity					693:705	cell length heterogeneity	681:705	cell length heterogeneity	681:705	We observed that deletion of prkC affects cell morphology with an increase in mean size, cell length heterogeneity, and presence of abnormal septa.
31085703	6	52	gly	heterogeneity	693:705	arg1	septa					733:737	abnormal septa	724:737	abnormal septa	724:737	We observed that deletion of prkC affects cell morphology with an increase in mean size, cell length heterogeneity, and presence of abnormal septa.
31085703	6	53	theme	abnormal	724:731	arg1	septa					733:737	abnormal septa	724:737	abnormal septa	724:737	We observed that deletion of prkC affects cell morphology with an increase in mean size, cell length heterogeneity, and presence of abnormal septa.
31085703	11	54	theme	proteins	1359:1366	arg1	majority					1334:1341	the majority	1330:1341	the majority of C. difficile proteins associated with the cell wall	1330:1396	A proteomic analysis showed that the majority of C. difficile proteins associated with the cell wall were less abundant in the ΔprkC mutant than the wild-type strain.
31085703	11	54	theme	proteins	1359:1366	arg1	abundant					1408:1415	abundant	1408:1415	abundant	1408:1415	A proteomic analysis showed that the majority of C. difficile proteins associated with the cell wall were less abundant in the ΔprkC mutant than the wild-type strain.
31085703	12	55	theme	ΔprkC	1510:1514	arg1	mutant					1516:1521	the ΔprkC mutant	1506:1521	the ΔprkC mutant	1506:1521	Finally, in a hamster model of infection, the ΔprkC mutant had a colonization delay that did not significantly affect overall virulence.
31085703	3	56	theme	Signal	314:319	arg1	networks					334:341	Signal transduction networks	314:341	Signal transduction networks involving serine/threonine kinases (STKs)	314:383	Signal transduction networks involving serine/threonine kinases (STKs) play key roles in adaptation, as they regulate numerous physiological processes.
31085703	11	57	from	abundant	1408:1415	arg1	mutant					1430:1435	the ΔprkC mutant	1420:1435	the ΔprkC mutant	1420:1435	A proteomic analysis showed that the majority of C. difficile proteins associated with the cell wall were less abundant in the ΔprkC mutant than the wild-type strain.
31085703	1	58	from	adults	197:202	arg1	cause					154:158	the leading cause	142:158	the leading cause of antibiotic-associated diarrhea in adults	142:202	Clostridium difficile is the leading cause of antibiotic-associated diarrhea in adults.
31085703	1	58	from	adults	197:202	arg1	difficile					129:137	Clostridium difficile	117:137	Clostridium difficile	117:137	Clostridium difficile is the leading cause of antibiotic-associated diarrhea in adults.
31085703	12	59	theme	overall	1582:1588	arg1	virulence					1590:1598	overall virulence	1582:1598	overall virulence	1582:1598	Finally, in a hamster model of infection, the ΔprkC mutant had a colonization delay that did not significantly affect overall virulence.
31085703	9	60	dep	peptidoglycan	1142:1154	arg1	composition					1177:1187	II composition	1174:1187	II composition	1174:1187	This increased susceptibility was not associated with differences in peptidoglycan or polysaccharide II composition.
31085703	11	61	theme	wild-type	1446:1454	arg1	strain					1456:1461	the wild-type strain	1442:1461	the wild-type strain	1442:1461	A proteomic analysis showed that the majority of C. difficile proteins associated with the cell wall were less abundant in the ΔprkC mutant than the wild-type strain.
31085703	10	62	contain	had	1216:1218	arg1	mutant					1209:1214	the ΔprkC mutant	1199:1214	the ΔprkC mutant	1199:1214	However, the ΔprkC mutant had less peptidoglycan and released more polysaccharide II into the supernatant.
31085703	10	62	contain	had	1216:1218	arg2	peptidoglycan					1225:1237	peptidoglycan	1225:1237	peptidoglycan	1225:1237	However, the ΔprkC mutant had less peptidoglycan and released more polysaccharide II into the supernatant.
31085703	1	63	theme	leading	146:152	arg1	cause					154:158	the leading cause	142:158	the leading cause of antibiotic-associated diarrhea in adults	142:202	Clostridium difficile is the leading cause of antibiotic-associated diarrhea in adults.
31085703	1	63	theme	leading	146:152	arg1	difficile					129:137	Clostridium difficile	117:137	Clostridium difficile	117:137	Clostridium difficile is the leading cause of antibiotic-associated diarrhea in adults.
31085703	6	64	theme	cell	634:637	arg1	morphology					639:648	cell morphology	634:648	cell morphology	634:648	We observed that deletion of prkC affects cell morphology with an increase in mean size, cell length heterogeneity, and presence of abnormal septa.
31085703	11	65	theme	C.	1346:1347	arg1	difficile					1349:1357	C. difficile	1346:1357	C. difficile proteins associated with the cell wall	1346:1396	A proteomic analysis showed that the majority of C. difficile proteins associated with the cell wall were less abundant in the ΔprkC mutant than the wild-type strain.
31085703	2	66	theme	host	252:255	arg1	environment					257:267	the host environment	248:267	the host environment	248:267	During infection, C. difficile must detect the host environment and induce an appropriate survival strategy.
31085703	1	67	from	diarrhea	185:192	arg1	adults					197:202	adults	197:202	adults	197:202	Clostridium difficile is the leading cause of antibiotic-associated diarrhea in adults.
31085703	7	68	theme	wild-type	844:852	arg1	strain					854:859	the wild-type strain	840:859	the wild-type strain	840:859	A ΔprkC mutant was able to sporulate and germinate but was less motile and formed more biofilm than the wild-type strain.
30663956	2	0	theme	Gram-negative	100:112	arg1	bacterium					157:165	A Gram-negative, aerobic, coccus-shaped, non-spore-forming bacterium	98:165	A Gram-negative, aerobic, coccus-shaped, non-spore-forming bacterium	98:165	A Gram-negative, aerobic, coccus-shaped, non-spore-forming bacterium, designated M2BS4Y-1T, was isolated from a surface-sterilized leaf of Acrostichum aureum collected from Guangxi Zhuang Autonomous Region, China and investigated by a polyphasic approach to determine its taxonomic position.
30663956	11	1	theme	phylogenetic	1603:1614	arg1	data					1648:1651	phylogenetic, chemotaxonomic and phenotyptic data	1603:1651	phylogenetic, chemotaxonomic and phenotyptic data	1603:1651	On the basis of phylogenetic, chemotaxonomic and phenotyptic data, strain M2BS4Y-1T can be characterized to represent a novel species of the genus Aureimonas, for which the name Aureimonas flava sp.
30663956	10	2	dep	C18 	1563:1566	arg1	C16 					1578:1581	C16 	1578:1581	C16 	1578:1581	The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, sulfoquinovosyldiacylglycerol, two unknown aminolipids, an unidentified phospholipid and four unidentified lipids, while the major fatty acids were C18 : 1ω7c and C16 : 0.
30663956	10	2	dep	C18 	1563:1566	arg1	 1ω7c					1568:1572	 1ω7c	1568:1572	 1ω7c	1568:1572	The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, sulfoquinovosyldiacylglycerol, two unknown aminolipids, an unidentified phospholipid and four unidentified lipids, while the major fatty acids were C18 : 1ω7c and C16 : 0.
30663956	10	2	dep	C18 	1563:1566	arg1	 0					1583:1584	 0	1583:1584	C18 : 1ω7c and C16 : 0	1563:1584	The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, sulfoquinovosyldiacylglycerol, two unknown aminolipids, an unidentified phospholipid and four unidentified lipids, while the major fatty acids were C18 : 1ω7c and C16 : 0.
30663956	4	3	theme	diffusible	532:541	arg1	pigments					543:550	no diffusible pigments	529:550	no diffusible pigments	529:550	Substrate mycelia and aerial mycelia were not formed, and no diffusible pigments were observed on the media tested.
30663956	5	4	theme	genus	717:721	arg1	Aureimonas					723:732	the genus Aureimonas	713:732	the genus Aureimonas	713:732	Phylogenetic analysis based on its 16S rRNA gene sequence showed that strain M2BS4Y-1T was most closely related to species of the genus Aureimonas, and shared the highest 16S rRNA gene sequence similarity of 97.79 % to Aureimonas phyllosphaerae DSM 25026T.
30663956	6	5	theme	average	848:854	arg1	ANI					877:879	ANI	877:879	ANI	877:879	The average nucleotide identity (ANI) and in silico DNA-DNA hybridization (DDH) values between strain M2BS4Y-1T and A.phyllosphaerae DSM 25026T were 83.7 % and 26.5 %, respectively.
30663956	6	5	theme	average	848:854	arg1	identity					867:874	The average nucleotide identity	844:874	The average nucleotide identity (ANI)	844:880	The average nucleotide identity (ANI) and in silico DNA-DNA hybridization (DDH) values between strain M2BS4Y-1T and A.phyllosphaerae DSM 25026T were 83.7 % and 26.5 %, respectively.
30663956	7	6	theme	DDH	1038:1040	arg1	values					1042:1047	The ANI and DDH values	1026:1047	The ANI and DDH values	1026:1047	The ANI and DDH values were below the recommended thresholds.
30663956	2	7	dep	Zhuang	279:284	arg1	Region					297:302	Autonomous Region	286:302	Guangxi Zhuang Autonomous Region	271:302	A Gram-negative, aerobic, coccus-shaped, non-spore-forming bacterium, designated M2BS4Y-1T, was isolated from a surface-sterilized leaf of Acrostichum aureum collected from Guangxi Zhuang Autonomous Region, China and investigated by a polyphasic approach to determine its taxonomic position.
30663956	11	8	theme	Aureimonas	1765:1774	arg1	sp					1782:1783	the name Aureimonas flava sp	1756:1783	the name Aureimonas flava sp	1756:1783	On the basis of phylogenetic, chemotaxonomic and phenotyptic data, strain M2BS4Y-1T can be characterized to represent a novel species of the genus Aureimonas, for which the name Aureimonas flava sp.
30663956	7	9	theme	recommended	1064:1074	arg1	thresholds					1076:1085	the recommended thresholds	1060:1085	the recommended thresholds	1060:1085	The ANI and DDH values were below the recommended thresholds.
30663956	4	10	theme	Substrate	471:479	arg1	mycelia					481:487	Substrate mycelia	471:487	Substrate mycelia	471:487	Substrate mycelia and aerial mycelia were not formed, and no diffusible pigments were observed on the media tested.
30663956	11	11	theme	phenotyptic	1636:1646	arg1	data					1648:1651	phylogenetic, chemotaxonomic and phenotyptic data	1603:1651	phylogenetic, chemotaxonomic and phenotyptic data	1603:1651	On the basis of phylogenetic, chemotaxonomic and phenotyptic data, strain M2BS4Y-1T can be characterized to represent a novel species of the genus Aureimonas, for which the name Aureimonas flava sp.
30663956	2	12	theme	Autonomous	286:295	arg1	Region					297:302	Autonomous Region	286:302	Guangxi Zhuang Autonomous Region	271:302	A Gram-negative, aerobic, coccus-shaped, non-spore-forming bacterium, designated M2BS4Y-1T, was isolated from a surface-sterilized leaf of Acrostichum aureum collected from Guangxi Zhuang Autonomous Region, China and investigated by a polyphasic approach to determine its taxonomic position.
30663956	9	13	contain	contained	1171:1179	arg2	acid					1201:1204	meso-diaminobutyric acid	1181:1204	meso-diaminobutyric acid	1181:1204	The cell-wall peptidoglycan contained meso-diaminobutyric acid and ubiquinone Q-10 was the respiratory lipoquinone.
30663956	9	13	contain	contained	1171:1179	arg1	peptidoglycan					1157:1169	The cell-wall peptidoglycan	1143:1169	The cell-wall peptidoglycan	1143:1169	The cell-wall peptidoglycan contained meso-diaminobutyric acid and ubiquinone Q-10 was the respiratory lipoquinone.
30663956	9	14	theme	respiratory	1234:1244	arg1	lipoquinone					1246:1256	the respiratory lipoquinone	1230:1256	the respiratory lipoquinone	1230:1256	The cell-wall peptidoglycan contained meso-diaminobutyric acid and ubiquinone Q-10 was the respiratory lipoquinone.
30663956	9	14	theme	respiratory	1234:1244	arg1	Q-10					1221:1224	ubiquinone Q-10	1210:1224	ubiquinone Q-10	1210:1224	The cell-wall peptidoglycan contained meso-diaminobutyric acid and ubiquinone Q-10 was the respiratory lipoquinone.
30663956	5	15	theme	16S	622:624	arg1	sequence					636:643	its 16S rRNA gene sequence	618:643	its 16S rRNA gene sequence	618:643	Phylogenetic analysis based on its 16S rRNA gene sequence showed that strain M2BS4Y-1T was most closely related to species of the genus Aureimonas, and shared the highest 16S rRNA gene sequence similarity of 97.79 % to Aureimonas phyllosphaerae DSM 25026T.
30663956	13	16	theme	=KCTC	1834:1838	arg1	M2BS4Y-1T					1823:1831	M2BS4Y-1T	1823:1831	M2BS4Y-1T (=KCTC 62837T=CGMCC 1.13747T)	1823:1861	The type strain is M2BS4Y-1T (=KCTC 62837T=CGMCC 1.13747T).
30663956	13	16	theme	=KCTC	1834:1838	arg1	1.13747T					1853:1860	=KCTC 62837T=CGMCC 1.13747T	1834:1860	=KCTC 62837T=CGMCC 1.13747T	1834:1860	The type strain is M2BS4Y-1T (=KCTC 62837T=CGMCC 1.13747T).
30663956	11	17	theme	strain	1654:1659	arg1	M2BS4Y-1T					1661:1669	strain M2BS4Y-1T	1654:1669	strain M2BS4Y-1T	1654:1669	On the basis of phylogenetic, chemotaxonomic and phenotyptic data, strain M2BS4Y-1T can be characterized to represent a novel species of the genus Aureimonas, for which the name Aureimonas flava sp.
30663956	11	18	theme	name	1760:1763	arg1	sp					1782:1783	the name Aureimonas flava sp	1756:1783	the name Aureimonas flava sp	1756:1783	On the basis of phylogenetic, chemotaxonomic and phenotyptic data, strain M2BS4Y-1T can be characterized to represent a novel species of the genus Aureimonas, for which the name Aureimonas flava sp.
30663956	3	19	theme	Strain	390:395	arg1	M2BS4Y-1T					397:405	Strain M2BS4Y-1T	390:405	Strain M2BS4Y-1T	390:405	Strain M2BS4Y-1T grew optimally with 1 % (w/v) NaCl, at 30 °C and at pH 7.0-8.0.
30663956	13	20	theme	62837T=CGMCC	1840:1851	arg1	M2BS4Y-1T					1823:1831	M2BS4Y-1T	1823:1831	M2BS4Y-1T (=KCTC 62837T=CGMCC 1.13747T)	1823:1861	The type strain is M2BS4Y-1T (=KCTC 62837T=CGMCC 1.13747T).
30663956	13	20	theme	62837T=CGMCC	1840:1851	arg1	1.13747T					1853:1860	=KCTC 62837T=CGMCC 1.13747T	1834:1860	=KCTC 62837T=CGMCC 1.13747T	1834:1860	The type strain is M2BS4Y-1T (=KCTC 62837T=CGMCC 1.13747T).
30663956	2	21	attach	isolated	194:201	arg1	leaf					229:232	a surface-sterilized leaf	208:232	a surface-sterilized leaf of Acrostichum aureum collected from Guangxi Zhuang Autonomous Region, China	208:309	A Gram-negative, aerobic, coccus-shaped, non-spore-forming bacterium, designated M2BS4Y-1T, was isolated from a surface-sterilized leaf of Acrostichum aureum collected from Guangxi Zhuang Autonomous Region, China and investigated by a polyphasic approach to determine its taxonomic position.
30663956	2	21	attach	isolated	194:201	arg2	bacterium					157:165	A Gram-negative, aerobic, coccus-shaped, non-spore-forming bacterium	98:165	A Gram-negative, aerobic, coccus-shaped, non-spore-forming bacterium	98:165	A Gram-negative, aerobic, coccus-shaped, non-spore-forming bacterium, designated M2BS4Y-1T, was isolated from a surface-sterilized leaf of Acrostichum aureum collected from Guangxi Zhuang Autonomous Region, China and investigated by a polyphasic approach to determine its taxonomic position.
30663956	0	22	theme	flava	11:15	arg1	sp					17:18	Aureimonas flava sp	0:18	Aureimonas flava sp.	0:19	Aureimonas flava sp.
30663956	8	23	theme	DNA	1092:1094	arg1	G+C content					1096:1106	The DNA G+C content	1088:1106	The DNA G+C content of strain M2BS4Y-1T	1088:1126	The DNA G+C content of strain M2BS4Y-1T was 70.0 mol%.
30663956	8	23	theme	DNA	1092:1094	arg1	%					1140:1140	70.0 mol%	1132:1140	70.0 mol%	1132:1140	The DNA G+C content of strain M2BS4Y-1T was 70.0 mol%.
30663956	5	24	theme	Phylogenetic	587:598	arg1	analysis					600:607	Phylogenetic analysis	587:607	Phylogenetic analysis based on its 16S rRNA gene sequence	587:643	Phylogenetic analysis based on its 16S rRNA gene sequence showed that strain M2BS4Y-1T was most closely related to species of the genus Aureimonas, and shared the highest 16S rRNA gene sequence similarity of 97.79 % to Aureimonas phyllosphaerae DSM 25026T.
30663956	1	25	theme	endophytic	35:44	arg1	bacterium					46:54	a novel endophytic bacterium	27:54	a novel endophytic bacterium	27:54	nov., a novel endophytic bacterium isolated from leaf of Acrostichum aureum.
30663956	1	25	theme	endophytic	35:44	arg1	nov.					21:24	nov.	21:24	nov.	21:24	nov., a novel endophytic bacterium isolated from leaf of Acrostichum aureum.
30663956	10	26	theme	fatty	1546:1550	arg1	C18 					1563:1566	C18 	1563:1566	C18 	1563:1566	The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, sulfoquinovosyldiacylglycerol, two unknown aminolipids, an unidentified phospholipid and four unidentified lipids, while the major fatty acids were C18 : 1ω7c and C16 : 0.
30663956	10	26	theme	fatty	1546:1550	arg1	acids					1552:1556	the major fatty acids	1536:1556	the major fatty acids	1536:1556	The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, sulfoquinovosyldiacylglycerol, two unknown aminolipids, an unidentified phospholipid and four unidentified lipids, while the major fatty acids were C18 : 1ω7c and C16 : 0.
30663956	3	27	theme	1 	427:428	arg1	%					429:429	%	429:429	%	429:429	Strain M2BS4Y-1T grew optimally with 1 % (w/v) NaCl, at 30 °C and at pH 7.0-8.0.
30663956	0	28	theme	Aureimonas	0:9	arg1	sp					17:18	Aureimonas flava sp	0:18	Aureimonas flava sp.	0:19	Aureimonas flava sp.
30663956	6	29	theme	hybridization	904:916	arg1	values					924:929	The average nucleotide identity (ANI) and in silico DNA-DNA hybridization (DDH) values	844:929	The average nucleotide identity (ANI) and in silico DNA-DNA hybridization (DDH) values between strain M2BS4Y-1T and A.phyllosphaerae DSM 25026T	844:986	The average nucleotide identity (ANI) and in silico DNA-DNA hybridization (DDH) values between strain M2BS4Y-1T and A.phyllosphaerae DSM 25026T were 83.7 % and 26.5 %, respectively.
30663956	6	29	theme	hybridization	904:916	arg1	DDH					919:921	DDH	919:921	DDH	919:921	The average nucleotide identity (ANI) and in silico DNA-DNA hybridization (DDH) values between strain M2BS4Y-1T and A.phyllosphaerae DSM 25026T were 83.7 % and 26.5 %, respectively.
30663956	10	30	theme	unidentified	1474:1485	arg1	phospholipid					1487:1498	an unidentified phospholipid	1471:1498	an unidentified phospholipid	1471:1498	The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, sulfoquinovosyldiacylglycerol, two unknown aminolipids, an unidentified phospholipid and four unidentified lipids, while the major fatty acids were C18 : 1ω7c and C16 : 0.
30663956	10	30	theme	unidentified	1474:1485	arg1	phosphatidylcholine					1332:1350	phosphatidylcholine	1332:1350	phosphatidylcholine	1332:1350	The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, sulfoquinovosyldiacylglycerol, two unknown aminolipids, an unidentified phospholipid and four unidentified lipids, while the major fatty acids were C18 : 1ω7c and C16 : 0.
30663956	9	31	theme	ubiquinone	1210:1219	arg1	lipoquinone					1246:1256	the respiratory lipoquinone	1230:1256	the respiratory lipoquinone	1230:1256	The cell-wall peptidoglycan contained meso-diaminobutyric acid and ubiquinone Q-10 was the respiratory lipoquinone.
30663956	9	31	theme	ubiquinone	1210:1219	arg1	Q-10					1221:1224	ubiquinone Q-10	1210:1224	ubiquinone Q-10	1210:1224	The cell-wall peptidoglycan contained meso-diaminobutyric acid and ubiquinone Q-10 was the respiratory lipoquinone.
30663956	13	32	theme	type	1808:1811	arg1	M2BS4Y-1T					1823:1831	M2BS4Y-1T	1823:1831	M2BS4Y-1T (=KCTC 62837T=CGMCC 1.13747T)	1823:1861	The type strain is M2BS4Y-1T (=KCTC 62837T=CGMCC 1.13747T).
30663956	13	32	theme	type	1808:1811	arg1	strain					1813:1818	The type strain	1804:1818	The type strain	1804:1818	The type strain is M2BS4Y-1T (=KCTC 62837T=CGMCC 1.13747T).
30663956	3	33	theme	%	429:429	arg1	NaCl					437:440	1 % (w/v) NaCl	427:440	1 % (w/v) NaCl	427:440	Strain M2BS4Y-1T grew optimally with 1 % (w/v) NaCl, at 30 °C and at pH 7.0-8.0.
30663956	5	34	theme	16S	758:760	arg1	similarity					781:790	the highest 16S rRNA gene sequence similarity	746:790	the highest 16S rRNA gene sequence similarity of 97.79 % to Aureimonas phyllosphaerae DSM 25026T	746:841	Phylogenetic analysis based on its 16S rRNA gene sequence showed that strain M2BS4Y-1T was most closely related to species of the genus Aureimonas, and shared the highest 16S rRNA gene sequence similarity of 97.79 % to Aureimonas phyllosphaerae DSM 25026T.
30663956	5	35	theme	highest	750:756	arg1	similarity					781:790	the highest 16S rRNA gene sequence similarity	746:790	the highest 16S rRNA gene sequence similarity of 97.79 % to Aureimonas phyllosphaerae DSM 25026T	746:841	Phylogenetic analysis based on its 16S rRNA gene sequence showed that strain M2BS4Y-1T was most closely related to species of the genus Aureimonas, and shared the highest 16S rRNA gene sequence similarity of 97.79 % to Aureimonas phyllosphaerae DSM 25026T.
30663956	8	36	theme	strain	1111:1116	arg1	M2BS4Y-1T					1118:1126	strain M2BS4Y-1T	1111:1126	strain M2BS4Y-1T	1111:1126	The DNA G+C content of strain M2BS4Y-1T was 70.0 mol%.
30663956	1	37	theme	Acrostichum	78:88	arg1	aureum					90:95	Acrostichum aureum	78:95	Acrostichum aureum	78:95	nov., a novel endophytic bacterium isolated from leaf of Acrostichum aureum.
30663956	5	38	theme	gene	767:770	arg1	similarity					781:790	the highest 16S rRNA gene sequence similarity	746:790	the highest 16S rRNA gene sequence similarity of 97.79 % to Aureimonas phyllosphaerae DSM 25026T	746:841	Phylogenetic analysis based on its 16S rRNA gene sequence showed that strain M2BS4Y-1T was most closely related to species of the genus Aureimonas, and shared the highest 16S rRNA gene sequence similarity of 97.79 % to Aureimonas phyllosphaerae DSM 25026T.
30663956	5	39	theme	rRNA	762:765	arg1	similarity					781:790	the highest 16S rRNA gene sequence similarity	746:790	the highest 16S rRNA gene sequence similarity of 97.79 % to Aureimonas phyllosphaerae DSM 25026T	746:841	Phylogenetic analysis based on its 16S rRNA gene sequence showed that strain M2BS4Y-1T was most closely related to species of the genus Aureimonas, and shared the highest 16S rRNA gene sequence similarity of 97.79 % to Aureimonas phyllosphaerae DSM 25026T.
30663956	11	40	theme	flava	1776:1780	arg1	sp					1782:1783	the name Aureimonas flava sp	1756:1783	the name Aureimonas flava sp	1756:1783	On the basis of phylogenetic, chemotaxonomic and phenotyptic data, strain M2BS4Y-1T can be characterized to represent a novel species of the genus Aureimonas, for which the name Aureimonas flava sp.
30663956	5	41	theme	strain	657:662	arg1	M2BS4Y-1T					664:672	strain M2BS4Y-1T	657:672	strain M2BS4Y-1T	657:672	Phylogenetic analysis based on its 16S rRNA gene sequence showed that strain M2BS4Y-1T was most closely related to species of the genus Aureimonas, and shared the highest 16S rRNA gene sequence similarity of 97.79 % to Aureimonas phyllosphaerae DSM 25026T.
30663956	1	42	theme	aureum	90:95	arg1	leaf					70:73	leaf	70:73	leaf of Acrostichum aureum	70:95	nov., a novel endophytic bacterium isolated from leaf of Acrostichum aureum.
30663956	6	43	theme	nucleotide	856:865	arg1	ANI					877:879	ANI	877:879	ANI	877:879	The average nucleotide identity (ANI) and in silico DNA-DNA hybridization (DDH) values between strain M2BS4Y-1T and A.phyllosphaerae DSM 25026T were 83.7 % and 26.5 %, respectively.
30663956	6	43	theme	nucleotide	856:865	arg1	identity					867:874	The average nucleotide identity	844:874	The average nucleotide identity (ANI)	844:880	The average nucleotide identity (ANI) and in silico DNA-DNA hybridization (DDH) values between strain M2BS4Y-1T and A.phyllosphaerae DSM 25026T were 83.7 % and 26.5 %, respectively.
30663956	5	44	theme	sequence	772:779	arg1	similarity					781:790	the highest 16S rRNA gene sequence similarity	746:790	the highest 16S rRNA gene sequence similarity of 97.79 % to Aureimonas phyllosphaerae DSM 25026T	746:841	Phylogenetic analysis based on its 16S rRNA gene sequence showed that strain M2BS4Y-1T was most closely related to species of the genus Aureimonas, and shared the highest 16S rRNA gene sequence similarity of 97.79 % to Aureimonas phyllosphaerae DSM 25026T.
30663956	5	45	theme	Aureimonas	723:732	arg1	species					702:708	species	702:708	species of the genus Aureimonas	702:732	Phylogenetic analysis based on its 16S rRNA gene sequence showed that strain M2BS4Y-1T was most closely related to species of the genus Aureimonas, and shared the highest 16S rRNA gene sequence similarity of 97.79 % to Aureimonas phyllosphaerae DSM 25026T.
30663956	6	46	theme	in	886:887	arg1	hybridization					904:916	in silico DNA-DNA hybridization	886:916	in silico DNA-DNA hybridization	886:916	The average nucleotide identity (ANI) and in silico DNA-DNA hybridization (DDH) values between strain M2BS4Y-1T and A.phyllosphaerae DSM 25026T were 83.7 % and 26.5 %, respectively.
30663956	4	47	located	observed	557:564	arg2	pigments					543:550	no diffusible pigments	529:550	no diffusible pigments	529:550	Substrate mycelia and aerial mycelia were not formed, and no diffusible pigments were observed on the media tested.
30663956	4	47	located	observed	557:564	arg1	media					573:577	the media	569:577	the media tested	569:584	Substrate mycelia and aerial mycelia were not formed, and no diffusible pigments were observed on the media tested.
30663956	2	48	theme	taxonomic	370:378	arg1	position					380:387	its taxonomic position	366:387	its taxonomic position	366:387	A Gram-negative, aerobic, coccus-shaped, non-spore-forming bacterium, designated M2BS4Y-1T, was isolated from a surface-sterilized leaf of Acrostichum aureum collected from Guangxi Zhuang Autonomous Region, China and investigated by a polyphasic approach to determine its taxonomic position.
30663956	6	49	theme	DSM	977:979	arg1	25026T					981:986	A.phyllosphaerae DSM 25026T	960:986	A.phyllosphaerae DSM 25026T	960:986	The average nucleotide identity (ANI) and in silico DNA-DNA hybridization (DDH) values between strain M2BS4Y-1T and A.phyllosphaerae DSM 25026T were 83.7 % and 26.5 %, respectively.
30663956	8	50	theme	M2BS4Y-1T	1118:1126	arg1	G+C content					1096:1106	The DNA G+C content	1088:1106	The DNA G+C content of strain M2BS4Y-1T	1088:1126	The DNA G+C content of strain M2BS4Y-1T was 70.0 mol%.
30663956	8	50	theme	M2BS4Y-1T	1118:1126	arg1	%					1140:1140	70.0 mol%	1132:1140	70.0 mol%	1132:1140	The DNA G+C content of strain M2BS4Y-1T was 70.0 mol%.
30663956	4	51	theme	aerial	493:498	arg1	mycelia					500:506	aerial mycelia	493:506	aerial mycelia	493:506	Substrate mycelia and aerial mycelia were not formed, and no diffusible pigments were observed on the media tested.
30663956	10	52	theme	unidentified	1509:1520	arg1	lipids					1522:1527	four unidentified lipids	1504:1527	four unidentified lipids	1504:1527	The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, sulfoquinovosyldiacylglycerol, two unknown aminolipids, an unidentified phospholipid and four unidentified lipids, while the major fatty acids were C18 : 1ω7c and C16 : 0.
30663956	9	53	theme	cell-wall	1147:1155	arg1	peptidoglycan					1157:1169	The cell-wall peptidoglycan	1143:1169	The cell-wall peptidoglycan	1143:1169	The cell-wall peptidoglycan contained meso-diaminobutyric acid and ubiquinone Q-10 was the respiratory lipoquinone.
30663956	2	54	theme	surface-sterilized	210:227	arg1	leaf					229:232	a surface-sterilized leaf	208:232	a surface-sterilized leaf of Acrostichum aureum collected from Guangxi Zhuang Autonomous Region, China	208:309	A Gram-negative, aerobic, coccus-shaped, non-spore-forming bacterium, designated M2BS4Y-1T, was isolated from a surface-sterilized leaf of Acrostichum aureum collected from Guangxi Zhuang Autonomous Region, China and investigated by a polyphasic approach to determine its taxonomic position.
30663956	6	55	theme	A.phyllosphaerae	960:975	arg1	25026T					981:986	A.phyllosphaerae DSM 25026T	960:986	A.phyllosphaerae DSM 25026T	960:986	The average nucleotide identity (ANI) and in silico DNA-DNA hybridization (DDH) values between strain M2BS4Y-1T and A.phyllosphaerae DSM 25026T were 83.7 % and 26.5 %, respectively.
30663956	10	56	theme	unknown	1450:1456	arg1	aminolipids					1458:1468	two unknown aminolipids	1446:1468	two unknown aminolipids	1446:1468	The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, sulfoquinovosyldiacylglycerol, two unknown aminolipids, an unidentified phospholipid and four unidentified lipids, while the major fatty acids were C18 : 1ω7c and C16 : 0.
30663956	10	56	theme	unknown	1450:1456	arg1	phosphatidylcholine					1332:1350	phosphatidylcholine	1332:1350	phosphatidylcholine	1332:1350	The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, sulfoquinovosyldiacylglycerol, two unknown aminolipids, an unidentified phospholipid and four unidentified lipids, while the major fatty acids were C18 : 1ω7c and C16 : 0.
30663956	5	57	theme	rRNA	626:629	arg1	sequence					636:643	its 16S rRNA gene sequence	618:643	its 16S rRNA gene sequence	618:643	Phylogenetic analysis based on its 16S rRNA gene sequence showed that strain M2BS4Y-1T was most closely related to species of the genus Aureimonas, and shared the highest 16S rRNA gene sequence similarity of 97.79 % to Aureimonas phyllosphaerae DSM 25026T.
30663956	6	58	theme	DNA-DNA	896:902	arg1	hybridization					904:916	in silico DNA-DNA hybridization	886:916	in silico DNA-DNA hybridization	886:916	The average nucleotide identity (ANI) and in silico DNA-DNA hybridization (DDH) values between strain M2BS4Y-1T and A.phyllosphaerae DSM 25026T were 83.7 % and 26.5 %, respectively.
30663956	11	59	theme	genus	1728:1732	arg1	Aureimonas					1734:1743	the genus Aureimonas	1724:1743	the genus Aureimonas	1724:1743	On the basis of phylogenetic, chemotaxonomic and phenotyptic data, strain M2BS4Y-1T can be characterized to represent a novel species of the genus Aureimonas, for which the name Aureimonas flava sp.
30663956	2	60	theme	polyphasic	333:342	arg1	approach					344:351	a polyphasic approach	331:351	a polyphasic approach to determine its taxonomic position	331:387	A Gram-negative, aerobic, coccus-shaped, non-spore-forming bacterium, designated M2BS4Y-1T, was isolated from a surface-sterilized leaf of Acrostichum aureum collected from Guangxi Zhuang Autonomous Region, China and investigated by a polyphasic approach to determine its taxonomic position.
30663956	8	61	theme	70.0 mol	1132:1139	arg1	G+C content					1096:1106	The DNA G+C content	1088:1106	The DNA G+C content of strain M2BS4Y-1T	1088:1126	The DNA G+C content of strain M2BS4Y-1T was 70.0 mol%.
30663956	8	61	theme	70.0 mol	1132:1139	arg1	%					1140:1140	70.0 mol%	1132:1140	70.0 mol%	1132:1140	The DNA G+C content of strain M2BS4Y-1T was 70.0 mol%.
30663956	11	62	dep	data	1648:1651	arg1	the					1590:1592	the	1590:1592	the	1590:1592	On the basis of phylogenetic, chemotaxonomic and phenotyptic data, strain M2BS4Y-1T can be characterized to represent a novel species of the genus Aureimonas, for which the name Aureimonas flava sp.
30663956	11	62	dep	data	1648:1651	arg1	basis					1594:1598	basis	1594:1598	basis	1594:1598	On the basis of phylogenetic, chemotaxonomic and phenotyptic data, strain M2BS4Y-1T can be characterized to represent a novel species of the genus Aureimonas, for which the name Aureimonas flava sp.
30663956	5	63	theme	gene	631:634	arg1	sequence					636:643	its 16S rRNA gene sequence	618:643	its 16S rRNA gene sequence	618:643	Phylogenetic analysis based on its 16S rRNA gene sequence showed that strain M2BS4Y-1T was most closely related to species of the genus Aureimonas, and shared the highest 16S rRNA gene sequence similarity of 97.79 % to Aureimonas phyllosphaerae DSM 25026T.
30663956	9	64	theme	meso-diaminobutyric	1181:1199	arg1	acid					1201:1204	meso-diaminobutyric acid	1181:1204	meso-diaminobutyric acid	1181:1204	The cell-wall peptidoglycan contained meso-diaminobutyric acid and ubiquinone Q-10 was the respiratory lipoquinone.
30663956	3	65	theme	pH	459:460	arg1	7.0-8.0					462:468	pH 7.0-8.0	459:468	pH 7.0-8.0	459:468	Strain M2BS4Y-1T grew optimally with 1 % (w/v) NaCl, at 30 °C and at pH 7.0-8.0.
30663956	2	66	dep	Gram-negative	100:112	arg1	non-spore-forming					139:155	non-spore-forming	139:155	non-spore-forming	139:155	A Gram-negative, aerobic, coccus-shaped, non-spore-forming bacterium, designated M2BS4Y-1T, was isolated from a surface-sterilized leaf of Acrostichum aureum collected from Guangxi Zhuang Autonomous Region, China and investigated by a polyphasic approach to determine its taxonomic position.
30663956	2	66	dep	Gram-negative	100:112	arg1	coccus-shaped					124:136	coccus-shaped	124:136	coccus-shaped	124:136	A Gram-negative, aerobic, coccus-shaped, non-spore-forming bacterium, designated M2BS4Y-1T, was isolated from a surface-sterilized leaf of Acrostichum aureum collected from Guangxi Zhuang Autonomous Region, China and investigated by a polyphasic approach to determine its taxonomic position.
30663956	2	66	dep	Gram-negative	100:112	arg1	aerobic					115:121	aerobic	115:121	aerobic	115:121	A Gram-negative, aerobic, coccus-shaped, non-spore-forming bacterium, designated M2BS4Y-1T, was isolated from a surface-sterilized leaf of Acrostichum aureum collected from Guangxi Zhuang Autonomous Region, China and investigated by a polyphasic approach to determine its taxonomic position.
30663956	10	67	theme	major	1540:1544	arg1	C18 					1563:1566	C18 	1563:1566	C18 	1563:1566	The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, sulfoquinovosyldiacylglycerol, two unknown aminolipids, an unidentified phospholipid and four unidentified lipids, while the major fatty acids were C18 : 1ω7c and C16 : 0.
30663956	10	67	theme	major	1540:1544	arg1	acids					1552:1556	the major fatty acids	1536:1556	the major fatty acids	1536:1556	The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, sulfoquinovosyldiacylglycerol, two unknown aminolipids, an unidentified phospholipid and four unidentified lipids, while the major fatty acids were C18 : 1ω7c and C16 : 0.
30663956	6	68	dep	in	886:887	arg1	silico					889:894	silico	889:894	silico	889:894	The average nucleotide identity (ANI) and in silico DNA-DNA hybridization (DDH) values between strain M2BS4Y-1T and A.phyllosphaerae DSM 25026T were 83.7 % and 26.5 %, respectively.
30663956	3	69	dep	%	429:429	arg1	w/v					432:434	w/v	432:434	w/v	432:434	Strain M2BS4Y-1T grew optimally with 1 % (w/v) NaCl, at 30 °C and at pH 7.0-8.0.
30663956	5	70	theme	Aureimonas	806:815	arg1	25026T					836:841	Aureimonas phyllosphaerae DSM 25026T	806:841	Aureimonas phyllosphaerae DSM 25026T	806:841	Phylogenetic analysis based on its 16S rRNA gene sequence showed that strain M2BS4Y-1T was most closely related to species of the genus Aureimonas, and shared the highest 16S rRNA gene sequence similarity of 97.79 % to Aureimonas phyllosphaerae DSM 25026T.
30663956	10	71	theme	polar	1263:1267	arg1	lipids					1269:1274	The polar lipids	1259:1274	The polar lipids	1259:1274	The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, sulfoquinovosyldiacylglycerol, two unknown aminolipids, an unidentified phospholipid and four unidentified lipids, while the major fatty acids were C18 : 1ω7c and C16 : 0.
30663956	11	72	theme	novel	1707:1711	arg1	species					1713:1719	a novel species	1705:1719	a novel species	1705:1719	On the basis of phylogenetic, chemotaxonomic and phenotyptic data, strain M2BS4Y-1T can be characterized to represent a novel species of the genus Aureimonas, for which the name Aureimonas flava sp.
30663956	2	73	theme	aureum	249:254	arg1	leaf					229:232	a surface-sterilized leaf	208:232	a surface-sterilized leaf of Acrostichum aureum collected from Guangxi Zhuang Autonomous Region, China	208:309	A Gram-negative, aerobic, coccus-shaped, non-spore-forming bacterium, designated M2BS4Y-1T, was isolated from a surface-sterilized leaf of Acrostichum aureum collected from Guangxi Zhuang Autonomous Region, China and investigated by a polyphasic approach to determine its taxonomic position.
30663956	11	74	theme	Aureimonas	1734:1743	arg1	species					1713:1719	a novel species	1705:1719	a novel species	1705:1719	On the basis of phylogenetic, chemotaxonomic and phenotyptic data, strain M2BS4Y-1T can be characterized to represent a novel species of the genus Aureimonas, for which the name Aureimonas flava sp.
30663956	11	75	theme	chemotaxonomic	1617:1630	arg1	data					1648:1651	phylogenetic, chemotaxonomic and phenotyptic data	1603:1651	phylogenetic, chemotaxonomic and phenotyptic data	1603:1651	On the basis of phylogenetic, chemotaxonomic and phenotyptic data, strain M2BS4Y-1T can be characterized to represent a novel species of the genus Aureimonas, for which the name Aureimonas flava sp.
30663956	5	76	theme	phyllosphaerae	817:830	arg1	25026T					836:841	Aureimonas phyllosphaerae DSM 25026T	806:841	Aureimonas phyllosphaerae DSM 25026T	806:841	Phylogenetic analysis based on its 16S rRNA gene sequence showed that strain M2BS4Y-1T was most closely related to species of the genus Aureimonas, and shared the highest 16S rRNA gene sequence similarity of 97.79 % to Aureimonas phyllosphaerae DSM 25026T.
30663956	5	77	theme	%	801:801	arg1	similarity					781:790	the highest 16S rRNA gene sequence similarity	746:790	the highest 16S rRNA gene sequence similarity of 97.79 % to Aureimonas phyllosphaerae DSM 25026T	746:841	Phylogenetic analysis based on its 16S rRNA gene sequence showed that strain M2BS4Y-1T was most closely related to species of the genus Aureimonas, and shared the highest 16S rRNA gene sequence similarity of 97.79 % to Aureimonas phyllosphaerae DSM 25026T.
30663956	6	78	theme	strain	939:944	arg1	M2BS4Y-1T					946:954	strain M2BS4Y-1T	939:954	strain M2BS4Y-1T	939:954	The average nucleotide identity (ANI) and in silico DNA-DNA hybridization (DDH) values between strain M2BS4Y-1T and A.phyllosphaerae DSM 25026T were 83.7 % and 26.5 %, respectively.
30663956	6	79	theme	identity	867:874	arg1	values					924:929	The average nucleotide identity (ANI) and in silico DNA-DNA hybridization (DDH) values	844:929	The average nucleotide identity (ANI) and in silico DNA-DNA hybridization (DDH) values between strain M2BS4Y-1T and A.phyllosphaerae DSM 25026T	844:986	The average nucleotide identity (ANI) and in silico DNA-DNA hybridization (DDH) values between strain M2BS4Y-1T and A.phyllosphaerae DSM 25026T were 83.7 % and 26.5 %, respectively.
30663956	6	79	theme	identity	867:874	arg1	DDH					919:921	DDH	919:921	DDH	919:921	The average nucleotide identity (ANI) and in silico DNA-DNA hybridization (DDH) values between strain M2BS4Y-1T and A.phyllosphaerae DSM 25026T were 83.7 % and 26.5 %, respectively.
30663956	1	80	theme	novel	29:33	arg1	bacterium					46:54	a novel endophytic bacterium	27:54	a novel endophytic bacterium	27:54	nov., a novel endophytic bacterium isolated from leaf of Acrostichum aureum.
30663956	1	80	theme	novel	29:33	arg1	nov.					21:24	nov.	21:24	nov.	21:24	nov., a novel endophytic bacterium isolated from leaf of Acrostichum aureum.
30663956	5	81	theme	DSM	832:834	arg1	25026T					836:841	Aureimonas phyllosphaerae DSM 25026T	806:841	Aureimonas phyllosphaerae DSM 25026T	806:841	Phylogenetic analysis based on its 16S rRNA gene sequence showed that strain M2BS4Y-1T was most closely related to species of the genus Aureimonas, and shared the highest 16S rRNA gene sequence similarity of 97.79 % to Aureimonas phyllosphaerae DSM 25026T.
30663956	7	82	theme	ANI	1030:1032	arg1	values					1042:1047	The ANI and DDH values	1026:1047	The ANI and DDH values	1026:1047	The ANI and DDH values were below the recommended thresholds.
29360990	8	0	theme	missense	1214:1221	arg1	mutations					1223:1231	pbp4 promoter and missense mutations	1196:1231	mutations	1223:1231	pbp4 promoter and missense mutations detected in CRB were reconstituted in a WT strain individually and in combination.
29360990	13	1	theme	antibiotic	1840:1849	arg1	treatment					1851:1859	antibiotic treatment	1840:1859	antibiotic treatment	1840:1859	A similar synergistic interaction of pbp4 mutations was observed in enhanced peptidoglycan cross-linking upon antibiotic treatment.
29360990	13	2	theme	pbp4	1767:1770	arg1	mutations					1772:1780	pbp4 mutations	1767:1780	pbp4 mutations	1767:1780	A similar synergistic interaction of pbp4 mutations was observed in enhanced peptidoglycan cross-linking upon antibiotic treatment.
29360990	1	3	theme	β-lactam	179:186	arg1	resistance					188:197	high-level β-lactam resistance	168:197	high-level β-lactam resistance in Staphylococcus aureus	168:222	Background PBP4 is typically considered unimportant for conferring high-level β-lactam resistance in Staphylococcus aureus.
29360990	12	4	theme	β-lactam	1656:1663	arg1	resistance					1665:1674	β-lactam resistance	1656:1674	β-lactam resistance	1656:1674	Reconstitution of PBP4 missense mutations alone did not impart β-lactam resistance, but did so in synergism with pbp4 promoter mutation.
29360990	0	5	theme	β-lactam	80:87	arg1	resistance					89:98	PBP4-mediated high-level β-lactam resistance	55:98	PBP4-mediated high-level β-lactam resistance	55:98	PBP4 activity and its overexpression are necessary for PBP4-mediated high-level β-lactam resistance.
29360990	4	6	theme	active	584:589	arg1	site					591:594	PBP4's active site	577:594	PBP4's active site	577:594	Mutations in the pbp4 promoter that up-regulate its expression and missense mutations that surround PBP4's active site were detected in high frequencies among passaged strains, suggesting PBP4 plays a key role in resistance.
29360990	12	7	theme	missense	1616:1623	arg1	mutations					1625:1633	PBP4 missense mutations	1611:1633	PBP4 missense mutations	1611:1633	Reconstitution of PBP4 missense mutations alone did not impart β-lactam resistance, but did so in synergism with pbp4 promoter mutation.
29360990	6	8	theme	β-lactam	892:899	arg1	resistance					901:910	high-level β-lactam resistance	881:910	high-level β-lactam resistance	881:910	Objectives To determine whether enzymatic activity of PBP4 is required for high-level β-lactam resistance and to investigate how the pbp4-associated mutations provide β-lactam resistance.
29360990	1	9	from	resistance	188:197	arg1	aureus					217:222	Staphylococcus aureus	202:222	Staphylococcus aureus	202:222	Background PBP4 is typically considered unimportant for conferring high-level β-lactam resistance in Staphylococcus aureus.
29360990	4	10	from	Mutations	477:485	arg1	promoter					499:506	the pbp4 promoter	490:506	the pbp4 promoter	490:506	Mutations in the pbp4 promoter that up-regulate its expression and missense mutations that surround PBP4's active site were detected in high frequencies among passaged strains, suggesting PBP4 plays a key role in resistance.
29360990	2	11	from	Mutations	225:233	arg1	PBP4					238:241	PBP4	238:241	PBP4	238:241	Mutations in PBP4 have been associated with β-lactam non-susceptibility among natural strains of S. aureus.
29360990	4	12	theme	missense	544:551	arg1	mutations					553:561	missense mutations	544:561	missense mutations that surround PBP4's active site	544:594	Mutations in the pbp4 promoter that up-regulate its expression and missense mutations that surround PBP4's active site were detected in high frequencies among passaged strains, suggesting PBP4 plays a key role in resistance.
29360990	10	13	theme	pbp4	1442:1445	arg1	mutants					1447:1453	the pbp4 mutants	1438:1453	the pbp4 mutants	1438:1453	Bacterial peptidoglycan composition of the pbp4 mutants was evaluated with and without antibiotic treatment using LC.
29360990	10	14	theme	antibiotic	1486:1495	arg1	treatment					1497:1505	antibiotic treatment	1486:1505	antibiotic treatment using LC	1486:1514	Bacterial peptidoglycan composition of the pbp4 mutants was evaluated with and without antibiotic treatment using LC.
29360990	2	15	theme	aureus	325:330	arg1	strains					311:317	natural strains	303:317	natural strains of S. aureus	303:330	Mutations in PBP4 have been associated with β-lactam non-susceptibility among natural strains of S. aureus.
29360990	11	16	theme	complete	1552:1559	arg1	susceptibility					1570:1583	complete β-lactam susceptibility	1552:1583	complete β-lactam susceptibility of CRB	1552:1590	Results PBP4 inactivation imparted complete β-lactam susceptibility of CRB.
29360990	13	17	located	observed	1786:1793	arg1	cross-linking					1821:1833	enhanced peptidoglycan cross-linking	1798:1833	enhanced peptidoglycan cross-linking	1798:1833	A similar synergistic interaction of pbp4 mutations was observed in enhanced peptidoglycan cross-linking upon antibiotic treatment.
29360990	13	17	located	observed	1786:1793	arg2	interaction					1752:1762	A similar synergistic interaction	1730:1762	A similar synergistic interaction of pbp4 mutations	1730:1780	A similar synergistic interaction of pbp4 mutations was observed in enhanced peptidoglycan cross-linking upon antibiotic treatment.
29360990	3	18	theme	high-level	380:389	arg1	resistance					400:409	high-level β-lactam resistance	380:409	high-level β-lactam resistance in laboratory-generated strains passaged in β-lactam antibiotics	380:474	We have previously shown that PBP4 can mediate high-level β-lactam resistance in laboratory-generated strains passaged in β-lactam antibiotics.
29360990	13	19	theme	enhanced	1798:1805	arg1	cross-linking					1821:1833	enhanced peptidoglycan cross-linking	1798:1833	enhanced peptidoglycan cross-linking	1798:1833	A similar synergistic interaction of pbp4 mutations was observed in enhanced peptidoglycan cross-linking upon antibiotic treatment.
29360990	14	20	dep	Conclusions	1862:1872	arg1	activity					1881:1888	activity	1881:1888	activity	1881:1888	Conclusions PBP4's activity and overexpression both contribute to high-level β-lactam resistance.
29360990	14	20	dep	Conclusions	1862:1872	arg1	overexpression					1894:1907	overexpression	1894:1907	overexpression	1894:1907	Conclusions PBP4's activity and overexpression both contribute to high-level β-lactam resistance.
29360990	14	20	dep	Conclusions	1862:1872	arg1	Conclusions					1862:1872	Conclusions PBP4's	1862:1879	Conclusions PBP4's activity and overexpression	1862:1907	Conclusions PBP4's activity and overexpression both contribute to high-level β-lactam resistance.
29360990	11	21	theme	β-lactam	1561:1568	arg1	susceptibility					1570:1583	complete β-lactam susceptibility	1552:1583	complete β-lactam susceptibility of CRB	1552:1590	Results PBP4 inactivation imparted complete β-lactam susceptibility of CRB.
29360990	3	22	theme	β-lactam	391:398	arg1	resistance					400:409	high-level β-lactam resistance	380:409	high-level β-lactam resistance in laboratory-generated strains passaged in β-lactam antibiotics	380:474	We have previously shown that PBP4 can mediate high-level β-lactam resistance in laboratory-generated strains passaged in β-lactam antibiotics.
29360990	6	23	theme	β-lactam	973:980	arg1	resistance					982:991	β-lactam resistance	973:991	β-lactam resistance	973:991	Objectives To determine whether enzymatic activity of PBP4 is required for high-level β-lactam resistance and to investigate how the pbp4-associated mutations provide β-lactam resistance.
29360990	13	24	theme	peptidoglycan	1807:1819	arg1	cross-linking					1821:1833	enhanced peptidoglycan cross-linking	1798:1833	enhanced peptidoglycan cross-linking	1798:1833	A similar synergistic interaction of pbp4 mutations was observed in enhanced peptidoglycan cross-linking upon antibiotic treatment.
29360990	8	25	located	detected	1233:1240	arg2	promoter					1201:1208	pbp4 promoter and missense mutations	1196:1231	promoter	1201:1208	pbp4 promoter and missense mutations detected in CRB were reconstituted in a WT strain individually and in combination.
29360990	8	25	located	detected	1233:1240	arg1	CRB					1245:1247	CRB	1245:1247	CRB	1245:1247	pbp4 promoter and missense mutations detected in CRB were reconstituted in a WT strain individually and in combination.
29360990	14	26	theme	β-lactam	1939:1946	arg1	resistance					1948:1957	high-level β-lactam resistance	1928:1957	high-level β-lactam resistance	1928:1957	Conclusions PBP4's activity and overexpression both contribute to high-level β-lactam resistance.
29360990	7	27	theme	well-studied	1161:1172	arg1	strain					1183:1188	a representative and well-studied passaged strain	1140:1188	a representative and well-studied passaged strain	1140:1188	Methods The catalytic activity of PBP4 was disabled through introduction of a serine to alanine point mutation in its active site (Ser-75→Ala) in a representative and well-studied passaged strain, CRB.
29360990	7	27	theme	well-studied	1161:1172	arg1	CRB					1191:1193	CRB	1191:1193	CRB	1191:1193	Methods The catalytic activity of PBP4 was disabled through introduction of a serine to alanine point mutation in its active site (Ser-75→Ala) in a representative and well-studied passaged strain, CRB.
29360990	6	28	theme	PBP4	860:863	arg1	activity					848:855	enzymatic activity	838:855	enzymatic activity of PBP4	838:863	Objectives To determine whether enzymatic activity of PBP4 is required for high-level β-lactam resistance and to investigate how the pbp4-associated mutations provide β-lactam resistance.
29360990	0	29	theme	PBP4	0:3	arg1	activity					5:12	PBP4 activity	0:12	PBP4 activity	0:12	PBP4 activity and its overexpression are necessary for PBP4-mediated high-level β-lactam resistance.
29360990	7	30	theme	passaged	1174:1181	arg1	strain					1183:1188	a representative and well-studied passaged strain	1140:1188	a representative and well-studied passaged strain	1140:1188	Methods The catalytic activity of PBP4 was disabled through introduction of a serine to alanine point mutation in its active site (Ser-75→Ala) in a representative and well-studied passaged strain, CRB.
29360990	7	30	theme	passaged	1174:1181	arg1	CRB					1191:1193	CRB	1191:1193	CRB	1191:1193	Methods The catalytic activity of PBP4 was disabled through introduction of a serine to alanine point mutation in its active site (Ser-75→Ala) in a representative and well-studied passaged strain, CRB.
29360990	1	31	theme	Background	101:110	arg1	PBP4					112:115	Background PBP4	101:115	Background PBP4	101:115	Background PBP4 is typically considered unimportant for conferring high-level β-lactam resistance in Staphylococcus aureus.
29360990	11	32	theme	CRB	1588:1590	arg1	susceptibility					1570:1583	complete β-lactam susceptibility	1552:1583	complete β-lactam susceptibility of CRB	1552:1590	Results PBP4 inactivation imparted complete β-lactam susceptibility of CRB.
29360990	9	33	theme	β-Lactam	1316:1323	arg1	resistance					1325:1334	β-Lactam resistance	1316:1334	β-Lactam resistance of the resultant strains	1316:1359	β-Lactam resistance of the resultant strains was evaluated by population analysis.
29360990	3	34	theme	laboratory-generated	414:433	arg1	strains					435:441	laboratory-generated strains	414:441	laboratory-generated strains passaged in β-lactam antibiotics	414:474	We have previously shown that PBP4 can mediate high-level β-lactam resistance in laboratory-generated strains passaged in β-lactam antibiotics.
29360990	4	35	theme	high	613:616	arg1	frequencies					618:628	high frequencies	613:628	high frequencies among passaged strains	613:651	Mutations in the pbp4 promoter that up-regulate its expression and missense mutations that surround PBP4's active site were detected in high frequencies among passaged strains, suggesting PBP4 plays a key role in resistance.
29360990	7	36	theme	representative	1142:1155	arg1	strain					1183:1188	a representative and well-studied passaged strain	1140:1188	a representative and well-studied passaged strain	1140:1188	Methods The catalytic activity of PBP4 was disabled through introduction of a serine to alanine point mutation in its active site (Ser-75→Ala) in a representative and well-studied passaged strain, CRB.
29360990	7	36	theme	representative	1142:1155	arg1	CRB					1191:1193	CRB	1191:1193	CRB	1191:1193	Methods The catalytic activity of PBP4 was disabled through introduction of a serine to alanine point mutation in its active site (Ser-75→Ala) in a representative and well-studied passaged strain, CRB.
29360990	7	37	theme	PBP4	1028:1031	arg1	activity					1016:1023	The catalytic activity	1002:1023	The catalytic activity of PBP4	1002:1031	Methods The catalytic activity of PBP4 was disabled through introduction of a serine to alanine point mutation in its active site (Ser-75→Ala) in a representative and well-studied passaged strain, CRB.
29360990	4	38	located	detected	601:608	arg2	Mutations					477:485	Mutations	477:485	Mutations in the pbp4 promoter that up-regulate its expression and missense mutations that surround PBP4's active site	477:594	Mutations in the pbp4 promoter that up-regulate its expression and missense mutations that surround PBP4's active site were detected in high frequencies among passaged strains, suggesting PBP4 plays a key role in resistance.
29360990	4	38	located	detected	601:608	arg1	frequencies					618:628	high frequencies	613:628	high frequencies among passaged strains	613:651	Mutations in the pbp4 promoter that up-regulate its expression and missense mutations that surround PBP4's active site were detected in high frequencies among passaged strains, suggesting PBP4 plays a key role in resistance.
29360990	7	39	dep	Methods	994:1000	arg1	disabled					1037:1044	disabled	1037:1044	was disabled through introduction of a serine to alanine point mutation in its active site (Ser-75→Ala) in a representative and well-studied passaged strain, CRB	1033:1193	Methods The catalytic activity of PBP4 was disabled through introduction of a serine to alanine point mutation in its active site (Ser-75→Ala) in a representative and well-studied passaged strain, CRB.
29360990	7	40	from	mutation	1096:1103	arg1	Ser-75→Ala					1125:1134	Ser-75→Ala	1125:1134	Ser-75→Ala	1125:1134	Methods The catalytic activity of PBP4 was disabled through introduction of a serine to alanine point mutation in its active site (Ser-75→Ala) in a representative and well-studied passaged strain, CRB.
29360990	7	40	from	mutation	1096:1103	arg1	site					1119:1122	its active site	1108:1122	its active site (Ser-75→Ala) in a representative and well-studied passaged strain, CRB	1108:1193	Methods The catalytic activity of PBP4 was disabled through introduction of a serine to alanine point mutation in its active site (Ser-75→Ala) in a representative and well-studied passaged strain, CRB.
29360990	8	41	theme	WT	1273:1274	arg1	strain					1276:1281	a WT strain	1271:1281	a WT strain individually and in combination	1271:1313	pbp4 promoter and missense mutations detected in CRB were reconstituted in a WT strain individually and in combination.
29360990	4	42	theme	passaged	636:643	arg1	strains					645:651	passaged strains	636:651	passaged strains	636:651	Mutations in the pbp4 promoter that up-regulate its expression and missense mutations that surround PBP4's active site were detected in high frequencies among passaged strains, suggesting PBP4 plays a key role in resistance.
29360990	5	43	theme	high-level	763:772	arg1	resistance					783:792	high-level β-lactam resistance	763:792	high-level β-lactam resistance	763:792	How these mutations participate in PBP4's ability to provide high-level β-lactam resistance is unknown.
29360990	10	44	theme	Bacterial	1399:1407	arg1	composition					1423:1433	Bacterial peptidoglycan composition	1399:1433	Bacterial peptidoglycan composition of the pbp4 mutants	1399:1453	Bacterial peptidoglycan composition of the pbp4 mutants was evaluated with and without antibiotic treatment using LC.
29360990	5	45	theme	β-lactam	774:781	arg1	resistance					783:792	high-level β-lactam resistance	763:792	high-level β-lactam resistance	763:792	How these mutations participate in PBP4's ability to provide high-level β-lactam resistance is unknown.
29360990	6	46	theme	pbp4-associated	939:953	arg1	mutations					955:963	the pbp4-associated mutations	935:963	the pbp4-associated mutations	935:963	Objectives To determine whether enzymatic activity of PBP4 is required for high-level β-lactam resistance and to investigate how the pbp4-associated mutations provide β-lactam resistance.
29360990	2	47	theme	β-lactam	269:276	arg1	non-susceptibility					278:295	β-lactam non-susceptibility	269:295	β-lactam non-susceptibility among natural strains of S. aureus	269:330	Mutations in PBP4 have been associated with β-lactam non-susceptibility among natural strains of S. aureus.
29360990	13	48	theme	similar	1732:1738	arg1	interaction					1752:1762	A similar synergistic interaction	1730:1762	A similar synergistic interaction of pbp4 mutations	1730:1780	A similar synergistic interaction of pbp4 mutations was observed in enhanced peptidoglycan cross-linking upon antibiotic treatment.
29360990	3	49	from	resistance	400:409	arg1	strains					435:441	laboratory-generated strains	414:441	laboratory-generated strains passaged in β-lactam antibiotics	414:474	We have previously shown that PBP4 can mediate high-level β-lactam resistance in laboratory-generated strains passaged in β-lactam antibiotics.
29360990	6	50	theme	enzymatic	838:846	arg1	activity					848:855	enzymatic activity	838:855	enzymatic activity of PBP4	838:863	Objectives To determine whether enzymatic activity of PBP4 is required for high-level β-lactam resistance and to investigate how the pbp4-associated mutations provide β-lactam resistance.
29360990	14	51	theme	high-level	1928:1937	arg1	resistance					1948:1957	high-level β-lactam resistance	1928:1957	high-level β-lactam resistance	1928:1957	Conclusions PBP4's activity and overexpression both contribute to high-level β-lactam resistance.
29360990	3	52	theme	β-lactam	455:462	arg1	antibiotics					464:474	β-lactam antibiotics	455:474	β-lactam antibiotics	455:474	We have previously shown that PBP4 can mediate high-level β-lactam resistance in laboratory-generated strains passaged in β-lactam antibiotics.
29360990	13	53	theme	synergistic	1740:1750	arg1	interaction					1752:1762	A similar synergistic interaction	1730:1762	A similar synergistic interaction of pbp4 mutations	1730:1780	A similar synergistic interaction of pbp4 mutations was observed in enhanced peptidoglycan cross-linking upon antibiotic treatment.
29360990	12	54	theme	PBP4	1611:1614	arg1	mutations					1625:1633	PBP4 missense mutations	1611:1633	PBP4 missense mutations	1611:1633	Reconstitution of PBP4 missense mutations alone did not impart β-lactam resistance, but did so in synergism with pbp4 promoter mutation.
29360990	7	55	theme	active	1112:1117	arg1	Ser-75→Ala					1125:1134	Ser-75→Ala	1125:1134	Ser-75→Ala	1125:1134	Methods The catalytic activity of PBP4 was disabled through introduction of a serine to alanine point mutation in its active site (Ser-75→Ala) in a representative and well-studied passaged strain, CRB.
29360990	7	55	theme	active	1112:1117	arg1	site					1119:1122	its active site	1108:1122	its active site (Ser-75→Ala) in a representative and well-studied passaged strain, CRB	1108:1193	Methods The catalytic activity of PBP4 was disabled through introduction of a serine to alanine point mutation in its active site (Ser-75→Ala) in a representative and well-studied passaged strain, CRB.
29360990	12	56	with	synergism	1691:1699	arg1	mutation					1720:1727	pbp4 promoter mutation	1706:1727	pbp4 promoter mutation	1706:1727	Reconstitution of PBP4 missense mutations alone did not impart β-lactam resistance, but did so in synergism with pbp4 promoter mutation.
29360990	12	57	theme	promoter	1711:1718	arg1	mutation					1720:1727	pbp4 promoter mutation	1706:1727	pbp4 promoter mutation	1706:1727	Reconstitution of PBP4 missense mutations alone did not impart β-lactam resistance, but did so in synergism with pbp4 promoter mutation.
29360990	13	58	theme	mutations	1772:1780	arg1	interaction					1752:1762	A similar synergistic interaction	1730:1762	A similar synergistic interaction of pbp4 mutations	1730:1780	A similar synergistic interaction of pbp4 mutations was observed in enhanced peptidoglycan cross-linking upon antibiotic treatment.
29360990	7	59	theme	catalytic	1006:1014	arg1	activity					1016:1023	The catalytic activity	1002:1023	The catalytic activity of PBP4	1002:1031	Methods The catalytic activity of PBP4 was disabled through introduction of a serine to alanine point mutation in its active site (Ser-75→Ala) in a representative and well-studied passaged strain, CRB.
29360990	12	60	theme	pbp4	1706:1709	arg1	mutation					1720:1727	pbp4 promoter mutation	1706:1727	pbp4 promoter mutation	1706:1727	Reconstitution of PBP4 missense mutations alone did not impart β-lactam resistance, but did so in synergism with pbp4 promoter mutation.
29360990	9	61	theme	resultant	1343:1351	arg1	strains					1353:1359	the resultant strains	1339:1359	the resultant strains	1339:1359	β-Lactam resistance of the resultant strains was evaluated by population analysis.
29360990	6	62	theme	high-level	881:890	arg1	resistance					901:910	high-level β-lactam resistance	881:910	high-level β-lactam resistance	881:910	Objectives To determine whether enzymatic activity of PBP4 is required for high-level β-lactam resistance and to investigate how the pbp4-associated mutations provide β-lactam resistance.
29360990	7	63	from	site	1119:1122	arg1	strain					1183:1188	a representative and well-studied passaged strain	1140:1188	a representative and well-studied passaged strain	1140:1188	Methods The catalytic activity of PBP4 was disabled through introduction of a serine to alanine point mutation in its active site (Ser-75→Ala) in a representative and well-studied passaged strain, CRB.
29360990	7	63	from	site	1119:1122	arg1	CRB					1191:1193	CRB	1191:1193	CRB	1191:1193	Methods The catalytic activity of PBP4 was disabled through introduction of a serine to alanine point mutation in its active site (Ser-75→Ala) in a representative and well-studied passaged strain, CRB.
29360990	8	64	theme	pbp4	1196:1199	arg1	promoter					1201:1208	pbp4 promoter and missense mutations	1196:1231	promoter	1201:1208	pbp4 promoter and missense mutations detected in CRB were reconstituted in a WT strain individually and in combination.
29360990	9	65	theme	strains	1353:1359	arg1	resistance					1325:1334	β-Lactam resistance	1316:1334	β-Lactam resistance of the resultant strains	1316:1359	β-Lactam resistance of the resultant strains was evaluated by population analysis.
29360990	2	66	theme	natural	303:309	arg1	strains					311:317	natural strains	303:317	natural strains of S. aureus	303:330	Mutations in PBP4 have been associated with β-lactam non-susceptibility among natural strains of S. aureus.
29360990	7	67	theme	alanine	1082:1088	arg1	mutation					1096:1103	alanine point mutation	1082:1103	alanine point mutation in its active site (Ser-75→Ala) in a representative and well-studied passaged strain, CRB	1082:1193	Methods The catalytic activity of PBP4 was disabled through introduction of a serine to alanine point mutation in its active site (Ser-75→Ala) in a representative and well-studied passaged strain, CRB.
29360990	10	68	theme	peptidoglycan	1409:1421	arg1	composition					1423:1433	Bacterial peptidoglycan composition	1399:1433	Bacterial peptidoglycan composition of the pbp4 mutants	1399:1453	Bacterial peptidoglycan composition of the pbp4 mutants was evaluated with and without antibiotic treatment using LC.
29360990	10	69	theme	mutants	1447:1453	arg1	composition					1423:1433	Bacterial peptidoglycan composition	1399:1433	Bacterial peptidoglycan composition of the pbp4 mutants	1399:1453	Bacterial peptidoglycan composition of the pbp4 mutants was evaluated with and without antibiotic treatment using LC.
29360990	12	70	theme	mutations	1625:1633	arg1	Reconstitution					1593:1606	Reconstitution	1593:1606	Reconstitution of PBP4 missense mutations	1593:1633	Reconstitution of PBP4 missense mutations alone did not impart β-lactam resistance, but did so in synergism with pbp4 promoter mutation.
29360990	0	71	theme	high-level	69:78	arg1	resistance					89:98	PBP4-mediated high-level β-lactam resistance	55:98	PBP4-mediated high-level β-lactam resistance	55:98	PBP4 activity and its overexpression are necessary for PBP4-mediated high-level β-lactam resistance.
29360990	4	72	theme	pbp4	494:497	arg1	promoter					499:506	the pbp4 promoter	490:506	the pbp4 promoter	490:506	Mutations in the pbp4 promoter that up-regulate its expression and missense mutations that surround PBP4's active site were detected in high frequencies among passaged strains, suggesting PBP4 plays a key role in resistance.
29360990	7	73	theme	point	1090:1094	arg1	mutation					1096:1103	alanine point mutation	1082:1103	alanine point mutation in its active site (Ser-75→Ala) in a representative and well-studied passaged strain, CRB	1082:1193	Methods The catalytic activity of PBP4 was disabled through introduction of a serine to alanine point mutation in its active site (Ser-75→Ala) in a representative and well-studied passaged strain, CRB.
29360990	0	74	theme	PBP4-mediated	55:67	arg1	resistance					89:98	PBP4-mediated high-level β-lactam resistance	55:98	PBP4-mediated high-level β-lactam resistance	55:98	PBP4 activity and its overexpression are necessary for PBP4-mediated high-level β-lactam resistance.
29360990	9	75	theme	population	1378:1387	arg1	analysis					1389:1396	population analysis	1378:1396	population analysis	1378:1396	β-Lactam resistance of the resultant strains was evaluated by population analysis.
29360990	7	76	theme	serine	1072:1077	arg1	introduction					1054:1065	introduction	1054:1065	introduction of a serine to alanine point mutation in its active site (Ser-75→Ala) in a representative and well-studied passaged strain, CRB	1054:1193	Methods The catalytic activity of PBP4 was disabled through introduction of a serine to alanine point mutation in its active site (Ser-75→Ala) in a representative and well-studied passaged strain, CRB.
29360990	1	77	theme	high-level	168:177	arg1	resistance					188:197	high-level β-lactam resistance	168:197	high-level β-lactam resistance in Staphylococcus aureus	168:222	Background PBP4 is typically considered unimportant for conferring high-level β-lactam resistance in Staphylococcus aureus.
29360990	4	78	theme	key	678:680	arg1	role					682:685	a key role	676:685	a key role	676:685	Mutations in the pbp4 promoter that up-regulate its expression and missense mutations that surround PBP4's active site were detected in high frequencies among passaged strains, suggesting PBP4 plays a key role in resistance.
31554129	1	0	from	fibers	211:216	arg1	rich					195:198	rich	195:198	rich	195:198	Potato fiber is a side product in starch manufacturing rich in dietary fibers such as pectin, cellulose, hemicellulose and resistant starch.
31554129	13	1	theme	probiotic	2014:2022	arg1	strains					2038:2044	probiotic Lactobacillus strains	2014:2044	probiotic Lactobacillus strains	2014:2044	The study demonstrated that FiberBind 400 had a potential to protect probiotic Lactobacillus strains during the passage through the gastrointestinal tract and selectively modulate the gut bacterial populations.
31554129	3	2	theme	gastric	511:517	arg1	conditions					519:528	simulated gastric conditions	501:528	simulated gastric conditions	501:528	This study investigated the effect of FiberBind 400, a commercial potato fiber product, on survival of probiotic Lactobacillus strains at simulated gastric conditions and on the composition and metabolic activity of the gut microbiota, using the TIM-2 colon model.
31554129	8	3	theme	16S	1150:1152	arg1	rRNA					1154:1157	16S rRNA	1150:1157	16S rRNA gene amplicon	1150:1171	Composition of the fecal microbiota in TIM-2 fermentations was assessed by high-throughput sequencing of 16S rRNA gene amplicon.
31554129	8	4	theme	gene	1159:1162	arg1	amplicon					1164:1171	16S rRNA gene amplicon	1150:1171	16S rRNA gene amplicon	1150:1171	Composition of the fecal microbiota in TIM-2 fermentations was assessed by high-throughput sequencing of 16S rRNA gene amplicon.
31554129	4	5	theme	Resistant	628:636	arg1	substrates					698:707	reference substrates	688:707	reference substrates	688:707	Resistant starch and native starch from potato were used as reference substrates.
31554129	4	5	theme	Resistant	628:636	arg1	starch					638:643	Resistant starch	628:643	Resistant starch	628:643	Resistant starch and native starch from potato were used as reference substrates.
31554129	4	5	theme	Resistant	628:636	arg1	starch					656:661	native starch	649:661	native starch	649:661	Resistant starch and native starch from potato were used as reference substrates.
31554129	14	6	theme	added	2248:2252	arg1	benefits					2265:2272	added biological benefits	2248:2272	added biological benefits	2248:2272	This knowledge can support application of potato fiber as a functional food ingredient with added biological benefits.
31554129	9	7	theme	FiberBind	1190:1198	arg1	Fermentation					1174:1185	Fermentation	1174:1185	Fermentation of FiberBind 400	1174:1202	Fermentation of FiberBind 400 resulted in more diverse microbial communities compared to starches.
31554129	1	8	theme	starch	174:179	arg1	manufacturing					181:193	starch manufacturing	174:193	starch manufacturing rich in dietary fibers such as pectin, cellulose, hemicellulose and resistant starch	174:278	Potato fiber is a side product in starch manufacturing rich in dietary fibers such as pectin, cellulose, hemicellulose and resistant starch.
31554129	6	9	theme	highest	908:914	arg1	effect					916:921	The highest effect	904:921	The highest effect	904:921	The highest effect was observed for L. fermentum PCC® and L. rhamnosus LGG®.
31554129	14	10	theme	functional	2216:2225	arg1	application					2183:2193	application	2183:2193	application of potato fiber	2183:2209	This knowledge can support application of potato fiber as a functional food ingredient with added biological benefits.
31554129	14	10	theme	functional	2216:2225	arg1	ingredient					2232:2241	a functional food ingredient	2214:2241	a functional food ingredient with added biological benefits	2214:2272	This knowledge can support application of potato fiber as a functional food ingredient with added biological benefits.
31554129	0	11	theme	microbiota	119:128	arg1	composition					96:106	composition	96:106	composition of the gut microbiota in vitro	96:137	Effect of potato fiber on survival of Lactobacillus species at simulated gastric conditions and composition of the gut microbiota in vitro.
31554129	0	11	theme	microbiota	119:128	arg1	conditions					81:90	simulated gastric conditions	63:90	simulated gastric conditions	63:90	Effect of potato fiber on survival of Lactobacillus species at simulated gastric conditions and composition of the gut microbiota in vitro.
31554129	1	12	theme	rich	195:198	arg1	manufacturing					181:193	starch manufacturing	174:193	starch manufacturing rich in dietary fibers such as pectin, cellulose, hemicellulose and resistant starch	174:278	Potato fiber is a side product in starch manufacturing rich in dietary fibers such as pectin, cellulose, hemicellulose and resistant starch.
31554129	9	13	theme	microbial	1229:1237	arg1	communities					1239:1249	more diverse microbial communities	1216:1249	more diverse microbial communities	1216:1249	Fermentation of FiberBind 400 resulted in more diverse microbial communities compared to starches.
31554129	0	14	from	composition	96:106	arg1	survival					26:33	survival	26:33	survival of Lactobacillus species at simulated gastric conditions and composition of the gut microbiota in vitro	26:137	Effect of potato fiber on survival of Lactobacillus species at simulated gastric conditions and composition of the gut microbiota in vitro.
31554129	11	15	theme	Faecalibacterium	1752:1767	arg1	prausnitzii					1769:1779	Faecalibacterium prausnitzii	1752:1779	Faecalibacterium prausnitzii	1752:1779	Shifts in other bacterial populations, such as increased abundances of Oscillospira, Enterococcus, Bacteroidales, Citrobacter, along with reduction of Roseburia, Ruminococcus, and Faecalibacterium prausnitzii were not significantly different between the substrates.
31554129	3	16	theme	microbiota	587:596	arg1	composition					541:551	composition	541:551	composition	541:551	This study investigated the effect of FiberBind 400, a commercial potato fiber product, on survival of probiotic Lactobacillus strains at simulated gastric conditions and on the composition and metabolic activity of the gut microbiota, using the TIM-2 colon model.
31554129	3	16	theme	microbiota	587:596	arg1	activity					567:574	metabolic activity	557:574	metabolic activity	557:574	This study investigated the effect of FiberBind 400, a commercial potato fiber product, on survival of probiotic Lactobacillus strains at simulated gastric conditions and on the composition and metabolic activity of the gut microbiota, using the TIM-2 colon model.
31554129	0	17	from	conditions	81:90	arg1	survival					26:33	survival	26:33	survival of Lactobacillus species at simulated gastric conditions and composition of the gut microbiota in vitro	26:137	Effect of potato fiber on survival of Lactobacillus species at simulated gastric conditions and composition of the gut microbiota in vitro.
31554129	0	18	theme	species	52:58	arg1	survival					26:33	survival	26:33	survival of Lactobacillus species at simulated gastric conditions and composition of the gut microbiota in vitro	26:137	Effect of potato fiber on survival of Lactobacillus species at simulated gastric conditions and composition of the gut microbiota in vitro.
31554129	13	19	theme	gut	2129:2131	arg1	populations					2143:2153	the gut bacterial populations	2125:2153	the gut bacterial populations	2125:2153	The study demonstrated that FiberBind 400 had a potential to protect probiotic Lactobacillus strains during the passage through the gastrointestinal tract and selectively modulate the gut bacterial populations.
31554129	5	20	theme	tested	771:776	arg1	RC-14®					847:852	RC-14®	847:852	RC-14®	847:852	FiberBind 400 had an ability to improve survival of the four tested strains, Lactobacillus fermentum PCC®, L. rhamnosus LGG®, L. reuteri RC-14® and L. paracasei F-19® in a strain-dependent way.
31554129	5	20	theme	tested	771:776	arg1	F-19®					871:875	F-19®	871:875	F-19®	871:875	FiberBind 400 had an ability to improve survival of the four tested strains, Lactobacillus fermentum PCC®, L. rhamnosus LGG®, L. reuteri RC-14® and L. paracasei F-19® in a strain-dependent way.
31554129	5	20	theme	tested	771:776	arg1	strains					778:784	the four tested strains	762:784	the four tested strains	762:784	FiberBind 400 had an ability to improve survival of the four tested strains, Lactobacillus fermentum PCC®, L. rhamnosus LGG®, L. reuteri RC-14® and L. paracasei F-19® in a strain-dependent way.
31554129	5	20	theme	tested	771:776	arg1	LGG®					830:833	LGG®	830:833	LGG®	830:833	FiberBind 400 had an ability to improve survival of the four tested strains, Lactobacillus fermentum PCC®, L. rhamnosus LGG®, L. reuteri RC-14® and L. paracasei F-19® in a strain-dependent way.
31554129	5	21	contain	had	724:726	arg2	ability					731:737	an ability to improve survival of the four tested strains, Lactobacillus fermentum PCC®, L. rhamnosus LGG®, L. reuteri RC-14® and L. paracasei F-19® in a strain-dependent way	728:901	an ability to improve survival of the four tested strains, Lactobacillus fermentum PCC®, L. rhamnosus LGG®, L. reuteri RC-14® and L. paracasei F-19® in a strain-dependent way	728:901	FiberBind 400 had an ability to improve survival of the four tested strains, Lactobacillus fermentum PCC®, L. rhamnosus LGG®, L. reuteri RC-14® and L. paracasei F-19® in a strain-dependent way.
31554129	5	21	contain	had	724:726	arg1	FiberBind					710:718	FiberBind 400	710:722	FiberBind 400	710:722	FiberBind 400 had an ability to improve survival of the four tested strains, Lactobacillus fermentum PCC®, L. rhamnosus LGG®, L. reuteri RC-14® and L. paracasei F-19® in a strain-dependent way.
31554129	14	22	theme	fiber	2205:2209	arg1	ingredient					2232:2241	a functional food ingredient	2214:2241	a functional food ingredient with added biological benefits	2214:2272	This knowledge can support application of potato fiber as a functional food ingredient with added biological benefits.
31554129	14	22	theme	fiber	2205:2209	arg1	application					2183:2193	application	2183:2193	application of potato fiber	2183:2209	This knowledge can support application of potato fiber as a functional food ingredient with added biological benefits.
31554129	0	23	theme	simulated	63:71	arg1	conditions					81:90	simulated gastric conditions	63:90	simulated gastric conditions	63:90	Effect of potato fiber on survival of Lactobacillus species at simulated gastric conditions and composition of the gut microbiota in vitro.
31554129	0	24	from	Effect	0:5	arg1	survival					26:33	survival	26:33	survival of Lactobacillus species at simulated gastric conditions and composition of the gut microbiota in vitro	26:137	Effect of potato fiber on survival of Lactobacillus species at simulated gastric conditions and composition of the gut microbiota in vitro.
31554129	14	25	with	ingredient	2232:2241	arg1	benefits					2265:2272	added biological benefits	2248:2272	added biological benefits	2248:2272	This knowledge can support application of potato fiber as a functional food ingredient with added biological benefits.
31554129	3	26	theme	FiberBind	401:409	arg1	effect					391:396	the effect	387:396	the effect of FiberBind 400, a commercial potato fiber product, on survival of probiotic Lactobacillus strains at simulated gastric conditions and on the composition and metabolic activity of the gut microbiota	387:596	This study investigated the effect of FiberBind 400, a commercial potato fiber product, on survival of probiotic Lactobacillus strains at simulated gastric conditions and on the composition and metabolic activity of the gut microbiota, using the TIM-2 colon model.
31554129	4	27	theme	reference	688:696	arg1	substrates					698:707	reference substrates	688:707	reference substrates	688:707	Resistant starch and native starch from potato were used as reference substrates.
31554129	4	27	theme	reference	688:696	arg1	starch					638:643	Resistant starch	628:643	Resistant starch	628:643	Resistant starch and native starch from potato were used as reference substrates.
31554129	4	27	theme	reference	688:696	arg1	starch					656:661	native starch	649:661	native starch	649:661	Resistant starch and native starch from potato were used as reference substrates.
31554129	3	28	theme	commercial	418:427	arg1	FiberBind					401:409	FiberBind 400	401:413	FiberBind 400	401:413	This study investigated the effect of FiberBind 400, a commercial potato fiber product, on survival of probiotic Lactobacillus strains at simulated gastric conditions and on the composition and metabolic activity of the gut microbiota, using the TIM-2 colon model.
31554129	3	28	theme	commercial	418:427	arg1	product					442:448	a commercial potato fiber product	416:448	a commercial potato fiber product	416:448	This study investigated the effect of FiberBind 400, a commercial potato fiber product, on survival of probiotic Lactobacillus strains at simulated gastric conditions and on the composition and metabolic activity of the gut microbiota, using the TIM-2 colon model.
31554129	0	29	theme	potato	10:15	arg1	fiber					17:21	potato fiber	10:21	potato fiber	10:21	Effect of potato fiber on survival of Lactobacillus species at simulated gastric conditions and composition of the gut microbiota in vitro.
31554129	3	30	theme	TIM-2	609:613	arg1	model					621:625	the TIM-2 colon model	605:625	the TIM-2 colon model	605:625	This study investigated the effect of FiberBind 400, a commercial potato fiber product, on survival of probiotic Lactobacillus strains at simulated gastric conditions and on the composition and metabolic activity of the gut microbiota, using the TIM-2 colon model.
31554129	10	31	theme	microbial	1284:1292	arg1	abundances					1294:1303	microbial abundances	1284:1303	microbial abundances specifically mediated by FiberBind 400	1284:1342	Changes in microbial abundances specifically mediated by FiberBind 400, included increases in the genera Lachnospira, Butyrivibrio, Mogibacterium, Parabacteroides, Prevotella and Desulfovibrio, and the species B. ovatus, as well as decreases in Ruminococcus torques and unassigned Ruminococcus spp.
31554129	12	32	theme	potato	1918:1923	arg1	fiber					1925:1929	potato fiber	1918:1929	potato fiber	1918:1929	Cumulative production of individual short-chain fatty acids was similar between potato fiber and starches.
31554129	10	33	theme	Ruminococcus	1518:1529	arg1	torques					1531:1537	Ruminococcus torques	1518:1537	Ruminococcus torques	1518:1537	Changes in microbial abundances specifically mediated by FiberBind 400, included increases in the genera Lachnospira, Butyrivibrio, Mogibacterium, Parabacteroides, Prevotella and Desulfovibrio, and the species B. ovatus, as well as decreases in Ruminococcus torques and unassigned Ruminococcus spp.
31554129	8	34	theme	fecal	1064:1068	arg1	microbiota					1070:1079	the fecal microbiota	1060:1079	the fecal microbiota	1060:1079	Composition of the fecal microbiota in TIM-2 fermentations was assessed by high-throughput sequencing of 16S rRNA gene amplicon.
31554129	12	35	theme	individual	1863:1872	arg1	acids					1892:1896	individual short-chain fatty acids	1863:1896	individual short-chain fatty acids	1863:1896	Cumulative production of individual short-chain fatty acids was similar between potato fiber and starches.
31554129	11	36	theme	Ruminococcus	1734:1745	arg1	reduction					1710:1718	reduction	1710:1718	reduction of Roseburia, Ruminococcus, and Faecalibacterium prausnitzii	1710:1779	Shifts in other bacterial populations, such as increased abundances of Oscillospira, Enterococcus, Bacteroidales, Citrobacter, along with reduction of Roseburia, Ruminococcus, and Faecalibacterium prausnitzii were not significantly different between the substrates.
31554129	11	37	theme	bacterial	1588:1596	arg1	populations					1598:1608	other bacterial populations	1582:1608	other bacterial populations	1582:1608	Shifts in other bacterial populations, such as increased abundances of Oscillospira, Enterococcus, Bacteroidales, Citrobacter, along with reduction of Roseburia, Ruminococcus, and Faecalibacterium prausnitzii were not significantly different between the substrates.
31554129	11	37	theme	bacterial	1588:1596	arg1	abundances					1629:1638	increased abundances	1619:1638	increased abundances of Oscillospira, Enterococcus, Bacteroidales, Citrobacter	1619:1696	Shifts in other bacterial populations, such as increased abundances of Oscillospira, Enterococcus, Bacteroidales, Citrobacter, along with reduction of Roseburia, Ruminococcus, and Faecalibacterium prausnitzii were not significantly different between the substrates.
31554129	3	38	theme	fiber	436:440	arg1	FiberBind					401:409	FiberBind 400	401:413	FiberBind 400	401:413	This study investigated the effect of FiberBind 400, a commercial potato fiber product, on survival of probiotic Lactobacillus strains at simulated gastric conditions and on the composition and metabolic activity of the gut microbiota, using the TIM-2 colon model.
31554129	3	38	theme	fiber	436:440	arg1	product					442:448	a commercial potato fiber product	416:448	a commercial potato fiber product	416:448	This study investigated the effect of FiberBind 400, a commercial potato fiber product, on survival of probiotic Lactobacillus strains at simulated gastric conditions and on the composition and metabolic activity of the gut microbiota, using the TIM-2 colon model.
31554129	12	39	theme	acids	1892:1896	arg1	production					1849:1858	Cumulative production	1838:1858	Cumulative production of individual short-chain fatty acids	1838:1896	Cumulative production of individual short-chain fatty acids was similar between potato fiber and starches.
31554129	10	40	theme	Ruminococcus	1554:1565	arg1	spp					1567:1569	unassigned Ruminococcus spp	1543:1569	unassigned Ruminococcus spp	1543:1569	Changes in microbial abundances specifically mediated by FiberBind 400, included increases in the genera Lachnospira, Butyrivibrio, Mogibacterium, Parabacteroides, Prevotella and Desulfovibrio, and the species B. ovatus, as well as decreases in Ruminococcus torques and unassigned Ruminococcus spp.
31554129	11	41	theme	Roseburia	1723:1731	arg1	reduction					1710:1718	reduction	1710:1718	reduction of Roseburia, Ruminococcus, and Faecalibacterium prausnitzii	1710:1779	Shifts in other bacterial populations, such as increased abundances of Oscillospira, Enterococcus, Bacteroidales, Citrobacter, along with reduction of Roseburia, Ruminococcus, and Faecalibacterium prausnitzii were not significantly different between the substrates.
31554129	5	42	dep	strains	778:784	arg1	RC-14®					847:852	RC-14®	847:852	RC-14®	847:852	FiberBind 400 had an ability to improve survival of the four tested strains, Lactobacillus fermentum PCC®, L. rhamnosus LGG®, L. reuteri RC-14® and L. paracasei F-19® in a strain-dependent way.
31554129	5	42	dep	strains	778:784	arg1	F-19®					871:875	F-19®	871:875	F-19®	871:875	FiberBind 400 had an ability to improve survival of the four tested strains, Lactobacillus fermentum PCC®, L. rhamnosus LGG®, L. reuteri RC-14® and L. paracasei F-19® in a strain-dependent way.
31554129	5	42	dep	strains	778:784	arg1	strains					778:784	the four tested strains	762:784	the four tested strains	762:784	FiberBind 400 had an ability to improve survival of the four tested strains, Lactobacillus fermentum PCC®, L. rhamnosus LGG®, L. reuteri RC-14® and L. paracasei F-19® in a strain-dependent way.
31554129	5	42	dep	strains	778:784	arg1	L. rhamnosus					817:828	L. rhamnosus	817:828	L. rhamnosus	817:828	FiberBind 400 had an ability to improve survival of the four tested strains, Lactobacillus fermentum PCC®, L. rhamnosus LGG®, L. reuteri RC-14® and L. paracasei F-19® in a strain-dependent way.
31554129	5	42	dep	strains	778:784	arg1	LGG®					830:833	LGG®	830:833	LGG®	830:833	FiberBind 400 had an ability to improve survival of the four tested strains, Lactobacillus fermentum PCC®, L. rhamnosus LGG®, L. reuteri RC-14® and L. paracasei F-19® in a strain-dependent way.
31554129	3	43	theme	probiotic	466:474	arg1	strains					490:496	probiotic Lactobacillus strains	466:496	probiotic Lactobacillus strains	466:496	This study investigated the effect of FiberBind 400, a commercial potato fiber product, on survival of probiotic Lactobacillus strains at simulated gastric conditions and on the composition and metabolic activity of the gut microbiota, using the TIM-2 colon model.
31554129	2	44	theme	potato	318:323	arg1	fiber					325:329	potato fiber	318:329	potato fiber	318:329	So far, the beneficial properties of potato fiber have been poorly characterized.
31554129	10	45	dep	genera	1371:1376	arg1	Mogibacterium					1405:1417	Mogibacterium	1405:1417	Mogibacterium	1405:1417	Changes in microbial abundances specifically mediated by FiberBind 400, included increases in the genera Lachnospira, Butyrivibrio, Mogibacterium, Parabacteroides, Prevotella and Desulfovibrio, and the species B. ovatus, as well as decreases in Ruminococcus torques and unassigned Ruminococcus spp.
31554129	10	45	dep	genera	1371:1376	arg1	B. ovatus					1483:1491	B. ovatus	1483:1491	B. ovatus	1483:1491	Changes in microbial abundances specifically mediated by FiberBind 400, included increases in the genera Lachnospira, Butyrivibrio, Mogibacterium, Parabacteroides, Prevotella and Desulfovibrio, and the species B. ovatus, as well as decreases in Ruminococcus torques and unassigned Ruminococcus spp.
31554129	10	45	dep	genera	1371:1376	arg1	Butyrivibrio					1391:1402	Butyrivibrio	1391:1402	Butyrivibrio	1391:1402	Changes in microbial abundances specifically mediated by FiberBind 400, included increases in the genera Lachnospira, Butyrivibrio, Mogibacterium, Parabacteroides, Prevotella and Desulfovibrio, and the species B. ovatus, as well as decreases in Ruminococcus torques and unassigned Ruminococcus spp.
31554129	10	45	dep	genera	1371:1376	arg1	Prevotella					1437:1446	Prevotella	1437:1446	Prevotella	1437:1446	Changes in microbial abundances specifically mediated by FiberBind 400, included increases in the genera Lachnospira, Butyrivibrio, Mogibacterium, Parabacteroides, Prevotella and Desulfovibrio, and the species B. ovatus, as well as decreases in Ruminococcus torques and unassigned Ruminococcus spp.
31554129	10	45	dep	genera	1371:1376	arg1	Parabacteroides					1420:1434	Parabacteroides	1420:1434	Parabacteroides	1420:1434	Changes in microbial abundances specifically mediated by FiberBind 400, included increases in the genera Lachnospira, Butyrivibrio, Mogibacterium, Parabacteroides, Prevotella and Desulfovibrio, and the species B. ovatus, as well as decreases in Ruminococcus torques and unassigned Ruminococcus spp.
31554129	11	46	theme	Oscillospira	1643:1654	arg1	abundances					1629:1638	increased abundances	1619:1638	increased abundances of Oscillospira, Enterococcus, Bacteroidales, Citrobacter	1619:1696	Shifts in other bacterial populations, such as increased abundances of Oscillospira, Enterococcus, Bacteroidales, Citrobacter, along with reduction of Roseburia, Ruminococcus, and Faecalibacterium prausnitzii were not significantly different between the substrates.
31554129	3	47	theme	simulated	501:509	arg1	conditions					519:528	simulated gastric conditions	501:528	simulated gastric conditions	501:528	This study investigated the effect of FiberBind 400, a commercial potato fiber product, on survival of probiotic Lactobacillus strains at simulated gastric conditions and on the composition and metabolic activity of the gut microbiota, using the TIM-2 colon model.
31554129	8	48	theme	high-throughput	1120:1134	arg1	sequencing					1136:1145	high-throughput sequencing	1120:1145	high-throughput sequencing of 16S rRNA gene amplicon	1120:1171	Composition of the fecal microbiota in TIM-2 fermentations was assessed by high-throughput sequencing of 16S rRNA gene amplicon.
31554129	13	49	theme	Lactobacillus	2024:2036	arg1	strains					2038:2044	probiotic Lactobacillus strains	2014:2044	probiotic Lactobacillus strains	2014:2044	The study demonstrated that FiberBind 400 had a potential to protect probiotic Lactobacillus strains during the passage through the gastrointestinal tract and selectively modulate the gut bacterial populations.
31554129	3	50	theme	strains	490:496	arg1	survival					454:461	survival	454:461	survival of probiotic Lactobacillus strains at simulated gastric conditions	454:528	This study investigated the effect of FiberBind 400, a commercial potato fiber product, on survival of probiotic Lactobacillus strains at simulated gastric conditions and on the composition and metabolic activity of the gut microbiota, using the TIM-2 colon model.
31554129	3	51	dep	composition	541:551	arg1	the					537:539	the	537:539	the	537:539	This study investigated the effect of FiberBind 400, a commercial potato fiber product, on survival of probiotic Lactobacillus strains at simulated gastric conditions and on the composition and metabolic activity of the gut microbiota, using the TIM-2 colon model.
31554129	14	52	theme	biological	2254:2263	arg1	benefits					2265:2272	added biological benefits	2248:2272	added biological benefits	2248:2272	This knowledge can support application of potato fiber as a functional food ingredient with added biological benefits.
31554129	12	53	theme	short-chain	1874:1884	arg1	acids					1892:1896	individual short-chain fatty acids	1863:1896	individual short-chain fatty acids	1863:1896	Cumulative production of individual short-chain fatty acids was similar between potato fiber and starches.
31554129	1	54	theme	side	158:161	arg1	product					163:169	a side product	156:169	a side product in starch manufacturing rich in dietary fibers such as pectin, cellulose, hemicellulose and resistant starch	156:278	Potato fiber is a side product in starch manufacturing rich in dietary fibers such as pectin, cellulose, hemicellulose and resistant starch.
31554129	1	54	theme	side	158:161	arg1	fiber					147:151	Potato fiber	140:151	Potato fiber	140:151	Potato fiber is a side product in starch manufacturing rich in dietary fibers such as pectin, cellulose, hemicellulose and resistant starch.
31554129	8	55	theme	rRNA	1154:1157	arg1	amplicon					1164:1171	16S rRNA gene amplicon	1150:1171	16S rRNA gene amplicon	1150:1171	Composition of the fecal microbiota in TIM-2 fermentations was assessed by high-throughput sequencing of 16S rRNA gene amplicon.
31554129	0	56	theme	gut	115:117	arg1	microbiota					119:128	the gut microbiota	111:128	the gut microbiota	111:128	Effect of potato fiber on survival of Lactobacillus species at simulated gastric conditions and composition of the gut microbiota in vitro.
31554129	5	57	theme	strains	778:784	arg1	survival					750:757	survival	750:757	survival of the four tested strains, Lactobacillus fermentum PCC®, L. rhamnosus LGG®, L. reuteri RC-14® and L. paracasei F-19®	750:875	FiberBind 400 had an ability to improve survival of the four tested strains, Lactobacillus fermentum PCC®, L. rhamnosus LGG®, L. reuteri RC-14® and L. paracasei F-19® in a strain-dependent way.
31554129	8	58	theme	amplicon	1164:1171	arg1	sequencing					1136:1145	high-throughput sequencing	1120:1145	high-throughput sequencing of 16S rRNA gene amplicon	1120:1171	Composition of the fecal microbiota in TIM-2 fermentations was assessed by high-throughput sequencing of 16S rRNA gene amplicon.
31554129	13	59	theme	gastrointestinal	2077:2092	arg1	tract					2094:2098	the gastrointestinal tract	2073:2098	the gastrointestinal tract	2073:2098	The study demonstrated that FiberBind 400 had a potential to protect probiotic Lactobacillus strains during the passage through the gastrointestinal tract and selectively modulate the gut bacterial populations.
31554129	14	60	theme	food	2227:2230	arg1	application					2183:2193	application	2183:2193	application of potato fiber	2183:2209	This knowledge can support application of potato fiber as a functional food ingredient with added biological benefits.
31554129	14	60	theme	food	2227:2230	arg1	ingredient					2232:2241	a functional food ingredient	2214:2241	a functional food ingredient with added biological benefits	2214:2272	This knowledge can support application of potato fiber as a functional food ingredient with added biological benefits.
31554129	7	61	theme	bacterial	1007:1015	arg1	survival					1017:1024	bacterial survival	1007:1024	bacterial survival	1007:1024	The effect of starches on bacterial survival was insignificant.
31554129	3	62	theme	metabolic	557:565	arg1	activity					567:574	metabolic activity	557:574	metabolic activity	557:574	This study investigated the effect of FiberBind 400, a commercial potato fiber product, on survival of probiotic Lactobacillus strains at simulated gastric conditions and on the composition and metabolic activity of the gut microbiota, using the TIM-2 colon model.
31554129	7	63	theme	starches	995:1002	arg1	effect					985:990	The effect	981:990	The effect of starches on bacterial survival	981:1024	The effect of starches on bacterial survival was insignificant.
31554129	7	63	theme	starches	995:1002	arg1	insignificant					1030:1042	insignificant	1030:1042	insignificant	1030:1042	The effect of starches on bacterial survival was insignificant.
31554129	9	64	theme	diverse	1221:1227	arg1	communities					1239:1249	more diverse microbial communities	1216:1249	more diverse microbial communities	1216:1249	Fermentation of FiberBind 400 resulted in more diverse microbial communities compared to starches.
31554129	12	65	theme	fatty	1886:1890	arg1	acids					1892:1896	individual short-chain fatty acids	1863:1896	individual short-chain fatty acids	1863:1896	Cumulative production of individual short-chain fatty acids was similar between potato fiber and starches.
31554129	1	66	from	rich	195:198	arg1	fibers					211:216	dietary fibers	203:216	dietary fibers such as pectin, cellulose, hemicellulose and resistant starch	203:278	Potato fiber is a side product in starch manufacturing rich in dietary fibers such as pectin, cellulose, hemicellulose and resistant starch.
31554129	1	66	from	rich	195:198	arg1	pectin					226:231	pectin	226:231	pectin	226:231	Potato fiber is a side product in starch manufacturing rich in dietary fibers such as pectin, cellulose, hemicellulose and resistant starch.
31554129	1	66	from	rich	195:198	arg1	hemicellulose					245:257	hemicellulose	245:257	hemicellulose	245:257	Potato fiber is a side product in starch manufacturing rich in dietary fibers such as pectin, cellulose, hemicellulose and resistant starch.
31554129	1	66	from	rich	195:198	arg1	starch					273:278	resistant starch	263:278	resistant starch	263:278	Potato fiber is a side product in starch manufacturing rich in dietary fibers such as pectin, cellulose, hemicellulose and resistant starch.
31554129	1	66	from	rich	195:198	arg1	cellulose					234:242	cellulose	234:242	cellulose	234:242	Potato fiber is a side product in starch manufacturing rich in dietary fibers such as pectin, cellulose, hemicellulose and resistant starch.
31554129	10	67	from	Changes	1273:1279	arg1	abundances					1294:1303	microbial abundances	1284:1303	microbial abundances specifically mediated by FiberBind 400	1284:1342	Changes in microbial abundances specifically mediated by FiberBind 400, included increases in the genera Lachnospira, Butyrivibrio, Mogibacterium, Parabacteroides, Prevotella and Desulfovibrio, and the species B. ovatus, as well as decreases in Ruminococcus torques and unassigned Ruminococcus spp.
31554129	3	68	from	conditions	519:528	arg1	survival					454:461	survival	454:461	survival of probiotic Lactobacillus strains at simulated gastric conditions	454:528	This study investigated the effect of FiberBind 400, a commercial potato fiber product, on survival of probiotic Lactobacillus strains at simulated gastric conditions and on the composition and metabolic activity of the gut microbiota, using the TIM-2 colon model.
31554129	3	69	theme	gut	583:585	arg1	microbiota					587:596	the gut microbiota	579:596	the gut microbiota	579:596	This study investigated the effect of FiberBind 400, a commercial potato fiber product, on survival of probiotic Lactobacillus strains at simulated gastric conditions and on the composition and metabolic activity of the gut microbiota, using the TIM-2 colon model.
31554129	11	70	from	Shifts	1572:1577	arg1	populations					1598:1608	other bacterial populations	1582:1608	other bacterial populations	1582:1608	Shifts in other bacterial populations, such as increased abundances of Oscillospira, Enterococcus, Bacteroidales, Citrobacter, along with reduction of Roseburia, Ruminococcus, and Faecalibacterium prausnitzii were not significantly different between the substrates.
31554129	11	70	from	Shifts	1572:1577	arg1	abundances					1629:1638	increased abundances	1619:1638	increased abundances of Oscillospira, Enterococcus, Bacteroidales, Citrobacter	1619:1696	Shifts in other bacterial populations, such as increased abundances of Oscillospira, Enterococcus, Bacteroidales, Citrobacter, along with reduction of Roseburia, Ruminococcus, and Faecalibacterium prausnitzii were not significantly different between the substrates.
31554129	11	71	theme	prausnitzii	1769:1779	arg1	reduction					1710:1718	reduction	1710:1718	reduction of Roseburia, Ruminococcus, and Faecalibacterium prausnitzii	1710:1779	Shifts in other bacterial populations, such as increased abundances of Oscillospira, Enterococcus, Bacteroidales, Citrobacter, along with reduction of Roseburia, Ruminococcus, and Faecalibacterium prausnitzii were not significantly different between the substrates.
31554129	1	72	from	product	163:169	arg1	manufacturing					181:193	starch manufacturing	174:193	starch manufacturing rich in dietary fibers such as pectin, cellulose, hemicellulose and resistant starch	174:278	Potato fiber is a side product in starch manufacturing rich in dietary fibers such as pectin, cellulose, hemicellulose and resistant starch.
31554129	8	73	from	Composition	1045:1055	arg1	fermentations					1090:1102	TIM-2 fermentations	1084:1102	TIM-2 fermentations	1084:1102	Composition of the fecal microbiota in TIM-2 fermentations was assessed by high-throughput sequencing of 16S rRNA gene amplicon.
31554129	0	74	theme	gastric	73:79	arg1	conditions					81:90	simulated gastric conditions	63:90	simulated gastric conditions	63:90	Effect of potato fiber on survival of Lactobacillus species at simulated gastric conditions and composition of the gut microbiota in vitro.
31554129	13	75	theme	bacterial	2133:2141	arg1	populations					2143:2153	the gut bacterial populations	2125:2153	the gut bacterial populations	2125:2153	The study demonstrated that FiberBind 400 had a potential to protect probiotic Lactobacillus strains during the passage through the gastrointestinal tract and selectively modulate the gut bacterial populations.
31554129	1	76	theme	Potato	140:145	arg1	product					163:169	a side product	156:169	a side product in starch manufacturing rich in dietary fibers such as pectin, cellulose, hemicellulose and resistant starch	156:278	Potato fiber is a side product in starch manufacturing rich in dietary fibers such as pectin, cellulose, hemicellulose and resistant starch.
31554129	1	76	theme	Potato	140:145	arg1	fiber					147:151	Potato fiber	140:151	Potato fiber	140:151	Potato fiber is a side product in starch manufacturing rich in dietary fibers such as pectin, cellulose, hemicellulose and resistant starch.
31554129	10	77	from	decreases	1505:1513	arg1	torques					1531:1537	Ruminococcus torques	1518:1537	Ruminococcus torques	1518:1537	Changes in microbial abundances specifically mediated by FiberBind 400, included increases in the genera Lachnospira, Butyrivibrio, Mogibacterium, Parabacteroides, Prevotella and Desulfovibrio, and the species B. ovatus, as well as decreases in Ruminococcus torques and unassigned Ruminococcus spp.
31554129	10	77	from	decreases	1505:1513	arg1	Desulfovibrio					1452:1464	Desulfovibrio	1452:1464	Desulfovibrio	1452:1464	Changes in microbial abundances specifically mediated by FiberBind 400, included increases in the genera Lachnospira, Butyrivibrio, Mogibacterium, Parabacteroides, Prevotella and Desulfovibrio, and the species B. ovatus, as well as decreases in Ruminococcus torques and unassigned Ruminococcus spp.
31554129	10	77	from	decreases	1505:1513	arg1	genera					1371:1376	the genera Lachnospira, Butyrivibrio, Mogibacterium, Parabacteroides, Prevotella and Desulfovibrio, and the species B. ovatus	1367:1491	genera	1371:1376	Changes in microbial abundances specifically mediated by FiberBind 400, included increases in the genera Lachnospira, Butyrivibrio, Mogibacterium, Parabacteroides, Prevotella and Desulfovibrio, and the species B. ovatus, as well as decreases in Ruminococcus torques and unassigned Ruminococcus spp.
31554129	10	77	from	decreases	1505:1513	arg1	spp					1567:1569	unassigned Ruminococcus spp	1543:1569	unassigned Ruminococcus spp	1543:1569	Changes in microbial abundances specifically mediated by FiberBind 400, included increases in the genera Lachnospira, Butyrivibrio, Mogibacterium, Parabacteroides, Prevotella and Desulfovibrio, and the species B. ovatus, as well as decreases in Ruminococcus torques and unassigned Ruminococcus spp.
31554129	10	77	from	decreases	1505:1513	arg1	species					1475:1481	the species	1471:1481	the species	1471:1481	Changes in microbial abundances specifically mediated by FiberBind 400, included increases in the genera Lachnospira, Butyrivibrio, Mogibacterium, Parabacteroides, Prevotella and Desulfovibrio, and the species B. ovatus, as well as decreases in Ruminococcus torques and unassigned Ruminococcus spp.
31554129	14	78	theme	potato	2198:2203	arg1	fiber					2205:2209	potato fiber	2198:2209	potato fiber	2198:2209	This knowledge can support application of potato fiber as a functional food ingredient with added biological benefits.
31554129	4	79	theme	native	649:654	arg1	substrates					698:707	reference substrates	688:707	reference substrates	688:707	Resistant starch and native starch from potato were used as reference substrates.
31554129	4	79	theme	native	649:654	arg1	starch					638:643	Resistant starch	628:643	Resistant starch	628:643	Resistant starch and native starch from potato were used as reference substrates.
31554129	4	79	theme	native	649:654	arg1	starch					656:661	native starch	649:661	native starch	649:661	Resistant starch and native starch from potato were used as reference substrates.
31554129	4	80	used	used	680:683	arg2	starch					638:643	Resistant starch	628:643	Resistant starch	628:643	Resistant starch and native starch from potato were used as reference substrates.
31554129	4	80	used	used	680:683	arg2	starch					656:661	native starch	649:661	native starch	649:661	Resistant starch and native starch from potato were used as reference substrates.
31554129	4	80	used	used	680:683	arg2	substrates					698:707	reference substrates	688:707	reference substrates	688:707	Resistant starch and native starch from potato were used as reference substrates.
31554129	3	81	from	effect	391:396	arg1	composition					541:551	composition	541:551	composition	541:551	This study investigated the effect of FiberBind 400, a commercial potato fiber product, on survival of probiotic Lactobacillus strains at simulated gastric conditions and on the composition and metabolic activity of the gut microbiota, using the TIM-2 colon model.
31554129	3	81	from	effect	391:396	arg1	activity					567:574	metabolic activity	557:574	metabolic activity	557:574	This study investigated the effect of FiberBind 400, a commercial potato fiber product, on survival of probiotic Lactobacillus strains at simulated gastric conditions and on the composition and metabolic activity of the gut microbiota, using the TIM-2 colon model.
31554129	3	81	from	effect	391:396	arg1	survival					454:461	survival	454:461	survival of probiotic Lactobacillus strains at simulated gastric conditions	454:528	This study investigated the effect of FiberBind 400, a commercial potato fiber product, on survival of probiotic Lactobacillus strains at simulated gastric conditions and on the composition and metabolic activity of the gut microbiota, using the TIM-2 colon model.
31554129	5	82	theme	strain-dependent	882:897	arg1	way					899:901	a strain-dependent way	880:901	a strain-dependent way	880:901	FiberBind 400 had an ability to improve survival of the four tested strains, Lactobacillus fermentum PCC®, L. rhamnosus LGG®, L. reuteri RC-14® and L. paracasei F-19® in a strain-dependent way.
31554129	0	83	theme	fiber	17:21	arg1	Effect					0:5	Effect	0:5	Effect of potato fiber on survival of Lactobacillus species at simulated gastric conditions and composition of the gut microbiota in vitro.	0:138	Effect of potato fiber on survival of Lactobacillus species at simulated gastric conditions and composition of the gut microbiota in vitro.
31554129	10	84	from	increases	1354:1362	arg1	torques					1531:1537	Ruminococcus torques	1518:1537	Ruminococcus torques	1518:1537	Changes in microbial abundances specifically mediated by FiberBind 400, included increases in the genera Lachnospira, Butyrivibrio, Mogibacterium, Parabacteroides, Prevotella and Desulfovibrio, and the species B. ovatus, as well as decreases in Ruminococcus torques and unassigned Ruminococcus spp.
31554129	10	84	from	increases	1354:1362	arg1	Desulfovibrio					1452:1464	Desulfovibrio	1452:1464	Desulfovibrio	1452:1464	Changes in microbial abundances specifically mediated by FiberBind 400, included increases in the genera Lachnospira, Butyrivibrio, Mogibacterium, Parabacteroides, Prevotella and Desulfovibrio, and the species B. ovatus, as well as decreases in Ruminococcus torques and unassigned Ruminococcus spp.
31554129	10	84	from	increases	1354:1362	arg1	genera					1371:1376	the genera Lachnospira, Butyrivibrio, Mogibacterium, Parabacteroides, Prevotella and Desulfovibrio, and the species B. ovatus	1367:1491	genera	1371:1376	Changes in microbial abundances specifically mediated by FiberBind 400, included increases in the genera Lachnospira, Butyrivibrio, Mogibacterium, Parabacteroides, Prevotella and Desulfovibrio, and the species B. ovatus, as well as decreases in Ruminococcus torques and unassigned Ruminococcus spp.
31554129	10	84	from	increases	1354:1362	arg1	spp					1567:1569	unassigned Ruminococcus spp	1543:1569	unassigned Ruminococcus spp	1543:1569	Changes in microbial abundances specifically mediated by FiberBind 400, included increases in the genera Lachnospira, Butyrivibrio, Mogibacterium, Parabacteroides, Prevotella and Desulfovibrio, and the species B. ovatus, as well as decreases in Ruminococcus torques and unassigned Ruminococcus spp.
31554129	10	84	from	increases	1354:1362	arg1	species					1475:1481	the species	1471:1481	the species	1471:1481	Changes in microbial abundances specifically mediated by FiberBind 400, included increases in the genera Lachnospira, Butyrivibrio, Mogibacterium, Parabacteroides, Prevotella and Desulfovibrio, and the species B. ovatus, as well as decreases in Ruminococcus torques and unassigned Ruminococcus spp.
31554129	3	85	theme	potato	429:434	arg1	FiberBind					401:409	FiberBind 400	401:413	FiberBind 400	401:413	This study investigated the effect of FiberBind 400, a commercial potato fiber product, on survival of probiotic Lactobacillus strains at simulated gastric conditions and on the composition and metabolic activity of the gut microbiota, using the TIM-2 colon model.
31554129	3	85	theme	potato	429:434	arg1	product					442:448	a commercial potato fiber product	416:448	a commercial potato fiber product	416:448	This study investigated the effect of FiberBind 400, a commercial potato fiber product, on survival of probiotic Lactobacillus strains at simulated gastric conditions and on the composition and metabolic activity of the gut microbiota, using the TIM-2 colon model.
31554129	1	86	theme	resistant	263:271	arg1	starch					273:278	resistant starch	263:278	resistant starch	263:278	Potato fiber is a side product in starch manufacturing rich in dietary fibers such as pectin, cellulose, hemicellulose and resistant starch.
31554129	0	87	theme	Lactobacillus	38:50	arg1	species					52:58	Lactobacillus species	38:58	Lactobacillus species	38:58	Effect of potato fiber on survival of Lactobacillus species at simulated gastric conditions and composition of the gut microbiota in vitro.
31554129	8	88	theme	microbiota	1070:1079	arg1	Composition					1045:1055	Composition	1045:1055	Composition of the fecal microbiota in TIM-2 fermentations	1045:1102	Composition of the fecal microbiota in TIM-2 fermentations was assessed by high-throughput sequencing of 16S rRNA gene amplicon.
31554129	12	89	theme	Cumulative	1838:1847	arg1	production					1849:1858	Cumulative production	1838:1858	Cumulative production of individual short-chain fatty acids	1838:1896	Cumulative production of individual short-chain fatty acids was similar between potato fiber and starches.
31554129	13	90	contain	had	1987:1989	arg2	potential					1993:2001	a potential to protect probiotic Lactobacillus strains during the passage through the gastrointestinal tract and selectively modulate the gut bacterial populations	1991:2153	a potential to protect probiotic Lactobacillus strains during the passage through the gastrointestinal tract and selectively modulate the gut bacterial populations	1991:2153	The study demonstrated that FiberBind 400 had a potential to protect probiotic Lactobacillus strains during the passage through the gastrointestinal tract and selectively modulate the gut bacterial populations.
31554129	13	90	contain	had	1987:1989	arg1	FiberBind					1973:1981	FiberBind 400	1973:1985	FiberBind 400	1973:1985	The study demonstrated that FiberBind 400 had a potential to protect probiotic Lactobacillus strains during the passage through the gastrointestinal tract and selectively modulate the gut bacterial populations.
31554129	11	91	theme	other	1582:1586	arg1	populations					1598:1608	other bacterial populations	1582:1608	other bacterial populations	1582:1608	Shifts in other bacterial populations, such as increased abundances of Oscillospira, Enterococcus, Bacteroidales, Citrobacter, along with reduction of Roseburia, Ruminococcus, and Faecalibacterium prausnitzii were not significantly different between the substrates.
31554129	11	91	theme	other	1582:1586	arg1	abundances					1629:1638	increased abundances	1619:1638	increased abundances of Oscillospira, Enterococcus, Bacteroidales, Citrobacter	1619:1696	Shifts in other bacterial populations, such as increased abundances of Oscillospira, Enterococcus, Bacteroidales, Citrobacter, along with reduction of Roseburia, Ruminococcus, and Faecalibacterium prausnitzii were not significantly different between the substrates.
31554129	2	92	theme	beneficial	293:302	arg1	properties					304:313	the beneficial properties	289:313	the beneficial properties of potato fiber	289:329	So far, the beneficial properties of potato fiber have been poorly characterized.
31554129	8	93	theme	TIM-2	1084:1088	arg1	fermentations					1090:1102	TIM-2 fermentations	1084:1102	TIM-2 fermentations	1084:1102	Composition of the fecal microbiota in TIM-2 fermentations was assessed by high-throughput sequencing of 16S rRNA gene amplicon.
31554129	7	94	from	effect	985:990	arg1	survival					1017:1024	bacterial survival	1007:1024	bacterial survival	1007:1024	The effect of starches on bacterial survival was insignificant.
31554129	3	95	theme	colon	615:619	arg1	model					621:625	the TIM-2 colon model	605:625	the TIM-2 colon model	605:625	This study investigated the effect of FiberBind 400, a commercial potato fiber product, on survival of probiotic Lactobacillus strains at simulated gastric conditions and on the composition and metabolic activity of the gut microbiota, using the TIM-2 colon model.
31554129	11	96	theme	increased	1619:1627	arg1	abundances					1629:1638	increased abundances	1619:1638	increased abundances of Oscillospira, Enterococcus, Bacteroidales, Citrobacter	1619:1696	Shifts in other bacterial populations, such as increased abundances of Oscillospira, Enterococcus, Bacteroidales, Citrobacter, along with reduction of Roseburia, Ruminococcus, and Faecalibacterium prausnitzii were not significantly different between the substrates.
31554129	2	97	theme	fiber	325:329	arg1	properties					304:313	the beneficial properties	289:313	the beneficial properties of potato fiber	289:329	So far, the beneficial properties of potato fiber have been poorly characterized.
31554129	4	98	from	potato	668:673	arg1	substrates					698:707	reference substrates	688:707	reference substrates	688:707	Resistant starch and native starch from potato were used as reference substrates.
31554129	4	98	from	potato	668:673	arg1	starch					638:643	Resistant starch	628:643	Resistant starch	628:643	Resistant starch and native starch from potato were used as reference substrates.
31554129	4	98	from	potato	668:673	arg1	starch					656:661	native starch	649:661	native starch	649:661	Resistant starch and native starch from potato were used as reference substrates.
31554129	1	99	theme	dietary	203:209	arg1	fibers					211:216	dietary fibers	203:216	dietary fibers such as pectin, cellulose, hemicellulose and resistant starch	203:278	Potato fiber is a side product in starch manufacturing rich in dietary fibers such as pectin, cellulose, hemicellulose and resistant starch.
31554129	1	99	theme	dietary	203:209	arg1	pectin					226:231	pectin	226:231	pectin	226:231	Potato fiber is a side product in starch manufacturing rich in dietary fibers such as pectin, cellulose, hemicellulose and resistant starch.
31554129	1	99	theme	dietary	203:209	arg1	hemicellulose					245:257	hemicellulose	245:257	hemicellulose	245:257	Potato fiber is a side product in starch manufacturing rich in dietary fibers such as pectin, cellulose, hemicellulose and resistant starch.
31554129	1	99	theme	dietary	203:209	arg1	starch					273:278	resistant starch	263:278	resistant starch	263:278	Potato fiber is a side product in starch manufacturing rich in dietary fibers such as pectin, cellulose, hemicellulose and resistant starch.
31554129	1	99	theme	dietary	203:209	arg1	cellulose					234:242	cellulose	234:242	cellulose	234:242	Potato fiber is a side product in starch manufacturing rich in dietary fibers such as pectin, cellulose, hemicellulose and resistant starch.
31554129	10	100	theme	unassigned	1543:1552	arg1	spp					1567:1569	unassigned Ruminococcus spp	1543:1569	unassigned Ruminococcus spp	1543:1569	Changes in microbial abundances specifically mediated by FiberBind 400, included increases in the genera Lachnospira, Butyrivibrio, Mogibacterium, Parabacteroides, Prevotella and Desulfovibrio, and the species B. ovatus, as well as decreases in Ruminococcus torques and unassigned Ruminococcus spp.
31554129	3	101	theme	Lactobacillus	476:488	arg1	strains					490:496	probiotic Lactobacillus strains	466:496	probiotic Lactobacillus strains	466:496	This study investigated the effect of FiberBind 400, a commercial potato fiber product, on survival of probiotic Lactobacillus strains at simulated gastric conditions and on the composition and metabolic activity of the gut microbiota, using the TIM-2 colon model.
29927366	3	0	theme	strain	266:271	arg1	position					250:257	The taxonomic position	236:257	The taxonomic position of the strain	236:271	The taxonomic position of the strain was investigated by using a polyphasic approach.
29927366	13	1	theme	phylogenetic	1266:1277	arg1	data					1279:1282	phenotypic, genotypic and phylogenetic data	1240:1282	phenotypic, genotypic and phylogenetic data	1240:1282	On the basis of phenotypic, genotypic and phylogenetic data, strain CFH 30205T merits representation of a novel species of the genus Nocardioides, for which the name Nocardioides allogilvus sp.
29927366	7	2	theme	strain	609:614	arg1	30205T					620:625	strain CFH 30205T	609:625	strain CFH 30205T	609:625	On the basis of 16S rRNA gene sequence analysis, strain CFH 30205T was most closely related to the type strains of Nocardioides terrigena DS-17T (97.6 % sequence similarity) and Nocardioides sediminis MSL-01T (97.0 %).
29927366	13	3	theme	data	1279:1282	arg1	merits					1303:1308	merits	1303:1308	merits	1303:1308	On the basis of phenotypic, genotypic and phylogenetic data, strain CFH 30205T merits representation of a novel species of the genus Nocardioides, for which the name Nocardioides allogilvus sp.
29927366	7	4	theme	Nocardioides	738:749	arg1	MSL-01T					761:767	Nocardioides sediminis MSL-01T	738:767	Nocardioides sediminis MSL-01T (97.0 %)	738:776	On the basis of 16S rRNA gene sequence analysis, strain CFH 30205T was most closely related to the type strains of Nocardioides terrigena DS-17T (97.6 % sequence similarity) and Nocardioides sediminis MSL-01T (97.0 %).
29927366	7	4	theme	Nocardioides	738:749	arg1	%					775:775	97.0 %	770:775	97.0 %	770:775	On the basis of 16S rRNA gene sequence analysis, strain CFH 30205T was most closely related to the type strains of Nocardioides terrigena DS-17T (97.6 % sequence similarity) and Nocardioides sediminis MSL-01T (97.0 %).
29927366	7	5	theme	CFH	616:618	arg1	30205T					620:625	strain CFH 30205T	609:625	strain CFH 30205T	609:625	On the basis of 16S rRNA gene sequence analysis, strain CFH 30205T was most closely related to the type strains of Nocardioides terrigena DS-17T (97.6 % sequence similarity) and Nocardioides sediminis MSL-01T (97.0 %).
29927366	3	6	theme	polyphasic	301:310	arg1	approach					312:319	a polyphasic approach	299:319	a polyphasic approach	299:319	The taxonomic position of the strain was investigated by using a polyphasic approach.
29927366	2	7	theme	novel	88:92	arg1	actinobacterium					94:108	A novel actinobacterium	86:108	A novel actinobacterium	86:108	A novel actinobacterium, designated strain CFH 30205T, was isolated from a soil sample collected from a karst cave in Luoyang, Henan Province, China.
29927366	15	8	theme	type	1440:1443	arg1	strain					1445:1450	The type strain	1436:1450	The type strain	1436:1450	The type strain is CFH 30205T (=KCTC 49020T=CGMCC 4.7457T).
29927366	15	8	theme	type	1440:1443	arg1	30205T					1459:1464	CFH 30205T	1455:1464	CFH 30205T (=KCTC 49020T=CGMCC 4.7457T)	1455:1493	The type strain is CFH 30205T (=KCTC 49020T=CGMCC 4.7457T).
29927366	4	9	theme	strain	335:340	arg1	Cells					322:326	Cells	322:326	Cells of the strain	322:340	Cells of the strain were aerobic, Gram-stain-positive, non-motile and rod-shaped.
29927366	7	10	theme	analysis	599:606	arg1	related					644:650	related	644:650	related	644:650	On the basis of 16S rRNA gene sequence analysis, strain CFH 30205T was most closely related to the type strains of Nocardioides terrigena DS-17T (97.6 % sequence similarity) and Nocardioides sediminis MSL-01T (97.0 %).
29927366	13	11	theme	allogilvus	1403:1412	arg1	sp					1414:1415	the name Nocardioides allogilvus sp	1381:1415	the name Nocardioides allogilvus sp	1381:1415	On the basis of phenotypic, genotypic and phylogenetic data, strain CFH 30205T merits representation of a novel species of the genus Nocardioides, for which the name Nocardioides allogilvus sp.
29927366	6	12	theme	CFH	470:472	arg1	30205T					474:479	Strain CFH 30205T	463:479	Strain CFH 30205T	463:479	Strain CFH 30205T grew optimally at 28 °C, pH 9.0 and in the presence of up to 1.5 % NaCl (w/v).
29927366	11	13	dep	iso-C16 	1063:1070	arg1	 0					1092:1093	 0	1092:1093	 0	1092:1093	The major isoprenoid quinone was MK-8 (H4), and the major fatty acids (>10 %) were iso-C16 : 0 and anteiso-C14 : 0.
29927366	11	13	dep	iso-C16 	1063:1070	arg1	anteiso-C14 					1079:1090	anteiso-C14 	1079:1090	anteiso-C14 	1079:1090	The major isoprenoid quinone was MK-8 (H4), and the major fatty acids (>10 %) were iso-C16 : 0 and anteiso-C14 : 0.
29927366	11	13	dep	iso-C16 	1063:1070	arg1	 0					1072:1073	 0	1072:1073	 0	1072:1073	The major isoprenoid quinone was MK-8 (H4), and the major fatty acids (>10 %) were iso-C16 : 0 and anteiso-C14 : 0.
29927366	12	14	theme	polar	1100:1104	arg1	lipids					1106:1111	The polar lipids	1096:1111	The polar lipids	1096:1111	The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unidentified phospholipid.
29927366	6	15	theme	Strain	463:468	arg1	30205T					474:479	Strain CFH 30205T	463:479	Strain CFH 30205T	463:479	Strain CFH 30205T grew optimally at 28 °C, pH 9.0 and in the presence of up to 1.5 % NaCl (w/v).
29927366	2	16	theme	CFH	129:131	arg1	30205T					133:138	strain CFH 30205T	122:138	strain CFH 30205T	122:138	A novel actinobacterium, designated strain CFH 30205T, was isolated from a soil sample collected from a karst cave in Luoyang, Henan Province, China.
29927366	7	17	dep	Nocardioides	738:749	arg1	sediminis					751:759	sediminis	751:759	sediminis	751:759	On the basis of 16S rRNA gene sequence analysis, strain CFH 30205T was most closely related to the type strains of Nocardioides terrigena DS-17T (97.6 % sequence similarity) and Nocardioides sediminis MSL-01T (97.0 %).
29927366	7	18	theme	sequence	713:720	arg1	similarity					722:731	97.6 % sequence similarity	706:731	97.6 % sequence similarity	706:731	On the basis of 16S rRNA gene sequence analysis, strain CFH 30205T was most closely related to the type strains of Nocardioides terrigena DS-17T (97.6 % sequence similarity) and Nocardioides sediminis MSL-01T (97.0 %).
29927366	7	18	theme	sequence	713:720	arg1	DS-17T					698:703	Nocardioides terrigena DS-17T	675:703	Nocardioides terrigena DS-17T (97.6 % sequence similarity)	675:732	On the basis of 16S rRNA gene sequence analysis, strain CFH 30205T was most closely related to the type strains of Nocardioides terrigena DS-17T (97.6 % sequence similarity) and Nocardioides sediminis MSL-01T (97.0 %).
29927366	13	19	theme	species	1336:1342	arg1	representation					1310:1323	representation	1310:1323	representation of a novel species of the genus Nocardioides, for which the name Nocardioides allogilvus sp	1310:1415	On the basis of phenotypic, genotypic and phylogenetic data, strain CFH 30205T merits representation of a novel species of the genus Nocardioides, for which the name Nocardioides allogilvus sp.
29927366	0	20	theme	allogilvus	13:22	arg1	sp					24:25	Nocardioides allogilvus sp	0:25	Nocardioides allogilvus sp.	0:26	Nocardioides allogilvus sp.
29927366	2	21	theme	strain	122:127	arg1	30205T					133:138	strain CFH 30205T	122:138	strain CFH 30205T	122:138	A novel actinobacterium, designated strain CFH 30205T, was isolated from a soil sample collected from a karst cave in Luoyang, Henan Province, China.
29927366	1	22	theme	karst	74:78	arg1	cave					80:83	a karst cave	72:83	a karst cave	72:83	nov., a novel actinobacterium isolated from a karst cave.
29927366	2	23	from	cave	196:199	arg1	Henan					213:217	Henan	213:217	Henan	213:217	A novel actinobacterium, designated strain CFH 30205T, was isolated from a soil sample collected from a karst cave in Luoyang, Henan Province, China.
29927366	2	23	from	cave	196:199	arg1	China					229:233	China	229:233	China	229:233	A novel actinobacterium, designated strain CFH 30205T, was isolated from a soil sample collected from a karst cave in Luoyang, Henan Province, China.
29927366	15	24	theme	=KCTC	1467:1471	arg1	4.7457T					1486:1492	=KCTC 49020T=CGMCC 4.7457T	1467:1492	=KCTC 49020T=CGMCC 4.7457T	1467:1492	The type strain is CFH 30205T (=KCTC 49020T=CGMCC 4.7457T).
29927366	15	24	theme	=KCTC	1467:1471	arg1	30205T					1459:1464	CFH 30205T	1455:1464	CFH 30205T (=KCTC 49020T=CGMCC 4.7457T)	1455:1493	The type strain is CFH 30205T (=KCTC 49020T=CGMCC 4.7457T).
29927366	0	25	theme	Nocardioides	0:11	arg1	sp					24:25	Nocardioides allogilvus sp	0:25	Nocardioides allogilvus sp.	0:26	Nocardioides allogilvus sp.
29927366	7	26	theme	Nocardioides	675:686	arg1	similarity					722:731	97.6 % sequence similarity	706:731	97.6 % sequence similarity	706:731	On the basis of 16S rRNA gene sequence analysis, strain CFH 30205T was most closely related to the type strains of Nocardioides terrigena DS-17T (97.6 % sequence similarity) and Nocardioides sediminis MSL-01T (97.0 %).
29927366	7	26	theme	Nocardioides	675:686	arg1	DS-17T					698:703	Nocardioides terrigena DS-17T	675:703	Nocardioides terrigena DS-17T (97.6 % sequence similarity)	675:732	On the basis of 16S rRNA gene sequence analysis, strain CFH 30205T was most closely related to the type strains of Nocardioides terrigena DS-17T (97.6 % sequence similarity) and Nocardioides sediminis MSL-01T (97.0 %).
29927366	15	27	theme	CFH	1455:1457	arg1	4.7457T					1486:1492	=KCTC 49020T=CGMCC 4.7457T	1467:1492	=KCTC 49020T=CGMCC 4.7457T	1467:1492	The type strain is CFH 30205T (=KCTC 49020T=CGMCC 4.7457T).
29927366	15	27	theme	CFH	1455:1457	arg1	strain					1445:1450	The type strain	1436:1450	The type strain	1436:1450	The type strain is CFH 30205T (=KCTC 49020T=CGMCC 4.7457T).
29927366	15	27	theme	CFH	1455:1457	arg1	30205T					1459:1464	CFH 30205T	1455:1464	CFH 30205T (=KCTC 49020T=CGMCC 4.7457T)	1455:1493	The type strain is CFH 30205T (=KCTC 49020T=CGMCC 4.7457T).
29927366	10	28	theme	whole-cell	926:935	arg1	mannose					949:955	mannose	949:955	mannose	949:955	The whole-cell sugars were mannose, xylose and galactose.
29927366	10	28	theme	whole-cell	926:935	arg1	sugars					937:942	The whole-cell sugars	922:942	The whole-cell sugars	922:942	The whole-cell sugars were mannose, xylose and galactose.
29927366	7	29	theme	97.6 	706:710	arg1	%					711:711	%	711:711	%	711:711	On the basis of 16S rRNA gene sequence analysis, strain CFH 30205T was most closely related to the type strains of Nocardioides terrigena DS-17T (97.6 % sequence similarity) and Nocardioides sediminis MSL-01T (97.0 %).
29927366	9	30	theme	ll-2,6-Diaminopimelic	831:851	arg1	acid					885:888	the diagnostic diamino acid	862:888	the diagnostic diamino acid in the cell-wall peptidoglycan	862:919	ll-2,6-Diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan.
29927366	9	30	theme	ll-2,6-Diaminopimelic	831:851	arg1	acid					853:856	ll-2,6-Diaminopimelic acid	831:856	ll-2,6-Diaminopimelic acid	831:856	ll-2,6-Diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan.
29927366	7	31	theme	terrigena	688:696	arg1	similarity					722:731	97.6 % sequence similarity	706:731	97.6 % sequence similarity	706:731	On the basis of 16S rRNA gene sequence analysis, strain CFH 30205T was most closely related to the type strains of Nocardioides terrigena DS-17T (97.6 % sequence similarity) and Nocardioides sediminis MSL-01T (97.0 %).
29927366	7	31	theme	terrigena	688:696	arg1	DS-17T					698:703	Nocardioides terrigena DS-17T	675:703	Nocardioides terrigena DS-17T (97.6 % sequence similarity)	675:732	On the basis of 16S rRNA gene sequence analysis, strain CFH 30205T was most closely related to the type strains of Nocardioides terrigena DS-17T (97.6 % sequence similarity) and Nocardioides sediminis MSL-01T (97.0 %).
29927366	13	32	theme	Nocardioides	1390:1401	arg1	sp					1414:1415	the name Nocardioides allogilvus sp	1381:1415	the name Nocardioides allogilvus sp	1381:1415	On the basis of phenotypic, genotypic and phylogenetic data, strain CFH 30205T merits representation of a novel species of the genus Nocardioides, for which the name Nocardioides allogilvus sp.
29927366	9	33	from	acid	885:888	arg1	peptidoglycan					907:919	the cell-wall peptidoglycan	893:919	the cell-wall peptidoglycan	893:919	ll-2,6-Diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan.
29927366	7	34	theme	%	711:711	arg1	similarity					722:731	97.6 % sequence similarity	706:731	97.6 % sequence similarity	706:731	On the basis of 16S rRNA gene sequence analysis, strain CFH 30205T was most closely related to the type strains of Nocardioides terrigena DS-17T (97.6 % sequence similarity) and Nocardioides sediminis MSL-01T (97.0 %).
29927366	7	34	theme	%	711:711	arg1	DS-17T					698:703	Nocardioides terrigena DS-17T	675:703	Nocardioides terrigena DS-17T (97.6 % sequence similarity)	675:732	On the basis of 16S rRNA gene sequence analysis, strain CFH 30205T was most closely related to the type strains of Nocardioides terrigena DS-17T (97.6 % sequence similarity) and Nocardioides sediminis MSL-01T (97.0 %).
29927366	13	35	dep	data	1279:1282	arg1	the					1227:1229	the	1227:1229	the	1227:1229	On the basis of phenotypic, genotypic and phylogenetic data, strain CFH 30205T merits representation of a novel species of the genus Nocardioides, for which the name Nocardioides allogilvus sp.
29927366	13	35	dep	data	1279:1282	arg1	basis					1231:1235	basis	1231:1235	basis	1231:1235	On the basis of phenotypic, genotypic and phylogenetic data, strain CFH 30205T merits representation of a novel species of the genus Nocardioides, for which the name Nocardioides allogilvus sp.
29927366	13	36	theme	phenotypic	1240:1249	arg1	data					1279:1282	phenotypic, genotypic and phylogenetic data	1240:1282	phenotypic, genotypic and phylogenetic data	1240:1282	On the basis of phenotypic, genotypic and phylogenetic data, strain CFH 30205T merits representation of a novel species of the genus Nocardioides, for which the name Nocardioides allogilvus sp.
29927366	7	37	theme	gene	585:588	arg1	analysis					599:606	16S rRNA gene sequence analysis	576:606	16S rRNA gene sequence analysis	576:606	On the basis of 16S rRNA gene sequence analysis, strain CFH 30205T was most closely related to the type strains of Nocardioides terrigena DS-17T (97.6 % sequence similarity) and Nocardioides sediminis MSL-01T (97.0 %).
29927366	11	38	theme	fatty	1038:1042	arg1	acids					1044:1048	the major fatty acids	1028:1048	the major fatty acids (>10 %)	1028:1056	The major isoprenoid quinone was MK-8 (H4), and the major fatty acids (>10 %) were iso-C16 : 0 and anteiso-C14 : 0.
29927366	11	38	theme	fatty	1038:1042	arg1	%					1055:1055	>10 %	1051:1055	>10 %	1051:1055	The major isoprenoid quinone was MK-8 (H4), and the major fatty acids (>10 %) were iso-C16 : 0 and anteiso-C14 : 0.
29927366	11	38	theme	fatty	1038:1042	arg1	iso-C16 					1063:1070	iso-C16 	1063:1070	iso-C16 	1063:1070	The major isoprenoid quinone was MK-8 (H4), and the major fatty acids (>10 %) were iso-C16 : 0 and anteiso-C14 : 0.
29927366	7	39	theme	DS-17T	698:703	arg1	strains					664:670	the type strains	655:670	the type strains of Nocardioides terrigena DS-17T (97.6 % sequence similarity) and Nocardioides sediminis MSL-01T (97.0 %)	655:776	On the basis of 16S rRNA gene sequence analysis, strain CFH 30205T was most closely related to the type strains of Nocardioides terrigena DS-17T (97.6 % sequence similarity) and Nocardioides sediminis MSL-01T (97.0 %).
29927366	7	40	theme	sequence	590:597	arg1	analysis					599:606	16S rRNA gene sequence analysis	576:606	16S rRNA gene sequence analysis	576:606	On the basis of 16S rRNA gene sequence analysis, strain CFH 30205T was most closely related to the type strains of Nocardioides terrigena DS-17T (97.6 % sequence similarity) and Nocardioides sediminis MSL-01T (97.0 %).
29927366	11	41	theme	isoprenoid	990:999	arg1	MK-8					1013:1016	MK-8	1013:1016	MK-8	1013:1016	The major isoprenoid quinone was MK-8 (H4), and the major fatty acids (>10 %) were iso-C16 : 0 and anteiso-C14 : 0.
29927366	11	41	theme	isoprenoid	990:999	arg1	quinone					1001:1007	The major isoprenoid quinone	980:1007	The major isoprenoid quinone	980:1007	The major isoprenoid quinone was MK-8 (H4), and the major fatty acids (>10 %) were iso-C16 : 0 and anteiso-C14 : 0.
29927366	12	42	theme	unidentified	1197:1208	arg1	phospholipid					1210:1221	an unidentified phospholipid	1194:1221	an unidentified phospholipid	1194:1221	The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unidentified phospholipid.
29927366	13	43	theme	novel	1330:1334	arg1	species					1336:1342	a novel species	1328:1342	a novel species	1328:1342	On the basis of phenotypic, genotypic and phylogenetic data, strain CFH 30205T merits representation of a novel species of the genus Nocardioides, for which the name Nocardioides allogilvus sp.
29927366	13	44	theme	Nocardioides	1357:1368	arg1	species					1336:1342	a novel species	1328:1342	a novel species	1328:1342	On the basis of phenotypic, genotypic and phylogenetic data, strain CFH 30205T merits representation of a novel species of the genus Nocardioides, for which the name Nocardioides allogilvus sp.
29927366	7	45	theme	16S	576:578	arg1	analysis					599:606	16S rRNA gene sequence analysis	576:606	16S rRNA gene sequence analysis	576:606	On the basis of 16S rRNA gene sequence analysis, strain CFH 30205T was most closely related to the type strains of Nocardioides terrigena DS-17T (97.6 % sequence similarity) and Nocardioides sediminis MSL-01T (97.0 %).
29927366	6	46	dep	1.5 	542:545	arg1	to					539:540	to	539:540	to	539:540	Strain CFH 30205T grew optimally at 28 °C, pH 9.0 and in the presence of up to 1.5 % NaCl (w/v).
29927366	2	47	attach	isolated	145:152	arg1	sample					166:171	a soil sample	159:171	a soil sample collected from a karst cave in Luoyang, Henan Province, China	159:233	A novel actinobacterium, designated strain CFH 30205T, was isolated from a soil sample collected from a karst cave in Luoyang, Henan Province, China.
29927366	2	47	attach	isolated	145:152	arg2	actinobacterium					94:108	A novel actinobacterium	86:108	A novel actinobacterium	86:108	A novel actinobacterium, designated strain CFH 30205T, was isolated from a soil sample collected from a karst cave in Luoyang, Henan Province, China.
29927366	9	48	theme	diagnostic	866:875	arg1	acid					885:888	the diagnostic diamino acid	862:888	the diagnostic diamino acid in the cell-wall peptidoglycan	862:919	ll-2,6-Diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan.
29927366	9	48	theme	diagnostic	866:875	arg1	acid					853:856	ll-2,6-Diaminopimelic acid	831:856	ll-2,6-Diaminopimelic acid	831:856	ll-2,6-Diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan.
29927366	7	49	theme	type	659:662	arg1	strains					664:670	the type strains	655:670	the type strains of Nocardioides terrigena DS-17T (97.6 % sequence similarity) and Nocardioides sediminis MSL-01T (97.0 %)	655:776	On the basis of 16S rRNA gene sequence analysis, strain CFH 30205T was most closely related to the type strains of Nocardioides terrigena DS-17T (97.6 % sequence similarity) and Nocardioides sediminis MSL-01T (97.0 %).
29927366	7	50	theme	rRNA	580:583	arg1	analysis					599:606	16S rRNA gene sequence analysis	576:606	16S rRNA gene sequence analysis	576:606	On the basis of 16S rRNA gene sequence analysis, strain CFH 30205T was most closely related to the type strains of Nocardioides terrigena DS-17T (97.6 % sequence similarity) and Nocardioides sediminis MSL-01T (97.0 %).
29927366	2	51	theme	karst	190:194	arg1	cave					196:199	a karst cave	188:199	a karst cave in Luoyang, Henan Province, China	188:233	A novel actinobacterium, designated strain CFH 30205T, was isolated from a soil sample collected from a karst cave in Luoyang, Henan Province, China.
29927366	15	52	theme	49020T=CGMCC	1473:1484	arg1	4.7457T					1486:1492	=KCTC 49020T=CGMCC 4.7457T	1467:1492	=KCTC 49020T=CGMCC 4.7457T	1467:1492	The type strain is CFH 30205T (=KCTC 49020T=CGMCC 4.7457T).
29927366	15	52	theme	49020T=CGMCC	1473:1484	arg1	30205T					1459:1464	CFH 30205T	1455:1464	CFH 30205T (=KCTC 49020T=CGMCC 4.7457T)	1455:1493	The type strain is CFH 30205T (=KCTC 49020T=CGMCC 4.7457T).
29927366	13	53	theme	name	1385:1388	arg1	sp					1414:1415	the name Nocardioides allogilvus sp	1381:1415	the name Nocardioides allogilvus sp	1381:1415	On the basis of phenotypic, genotypic and phylogenetic data, strain CFH 30205T merits representation of a novel species of the genus Nocardioides, for which the name Nocardioides allogilvus sp.
29927366	9	54	theme	diamino	877:883	arg1	acid					885:888	the diagnostic diamino acid	862:888	the diagnostic diamino acid in the cell-wall peptidoglycan	862:919	ll-2,6-Diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan.
29927366	9	54	theme	diamino	877:883	arg1	acid					853:856	ll-2,6-Diaminopimelic acid	831:856	ll-2,6-Diaminopimelic acid	831:856	ll-2,6-Diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan.
29927366	7	55	dep	analysis	599:606	arg1	basis					567:571	basis	567:571	basis	567:571	On the basis of 16S rRNA gene sequence analysis, strain CFH 30205T was most closely related to the type strains of Nocardioides terrigena DS-17T (97.6 % sequence similarity) and Nocardioides sediminis MSL-01T (97.0 %).
29927366	7	55	dep	analysis	599:606	arg1	the					563:565	the	563:565	the	563:565	On the basis of 16S rRNA gene sequence analysis, strain CFH 30205T was most closely related to the type strains of Nocardioides terrigena DS-17T (97.6 % sequence similarity) and Nocardioides sediminis MSL-01T (97.0 %).
29927366	11	56	theme	major	984:988	arg1	MK-8					1013:1016	MK-8	1013:1016	MK-8	1013:1016	The major isoprenoid quinone was MK-8 (H4), and the major fatty acids (>10 %) were iso-C16 : 0 and anteiso-C14 : 0.
29927366	11	56	theme	major	984:988	arg1	quinone					1001:1007	The major isoprenoid quinone	980:1007	The major isoprenoid quinone	980:1007	The major isoprenoid quinone was MK-8 (H4), and the major fatty acids (>10 %) were iso-C16 : 0 and anteiso-C14 : 0.
29927366	6	57	dep	NaCl	548:551	arg1	%					546:546	%	546:546	%	546:546	Strain CFH 30205T grew optimally at 28 °C, pH 9.0 and in the presence of up to 1.5 % NaCl (w/v).
29927366	13	58	theme	strain	1285:1290	arg1	30205T					1296:1301	strain CFH 30205T	1285:1301	strain CFH 30205T	1285:1301	On the basis of phenotypic, genotypic and phylogenetic data, strain CFH 30205T merits representation of a novel species of the genus Nocardioides, for which the name Nocardioides allogilvus sp.
29927366	3	59	theme	taxonomic	240:248	arg1	position					250:257	The taxonomic position	236:257	The taxonomic position of the strain	236:271	The taxonomic position of the strain was investigated by using a polyphasic approach.
29927366	13	60	theme	genus	1351:1355	arg1	Nocardioides					1357:1368	the genus Nocardioides	1347:1368	the genus Nocardioides	1347:1368	On the basis of phenotypic, genotypic and phylogenetic data, strain CFH 30205T merits representation of a novel species of the genus Nocardioides, for which the name Nocardioides allogilvus sp.
29927366	6	61	dep	%	546:546	arg1	1.5 					542:545	1.5 	542:545	1.5 	542:545	Strain CFH 30205T grew optimally at 28 °C, pH 9.0 and in the presence of up to 1.5 % NaCl (w/v).
29927366	13	62	theme	CFH	1292:1294	arg1	30205T					1296:1301	strain CFH 30205T	1285:1301	strain CFH 30205T	1285:1301	On the basis of phenotypic, genotypic and phylogenetic data, strain CFH 30205T merits representation of a novel species of the genus Nocardioides, for which the name Nocardioides allogilvus sp.
29927366	11	63	theme	major	1032:1036	arg1	acids					1044:1048	the major fatty acids	1028:1048	the major fatty acids (>10 %)	1028:1056	The major isoprenoid quinone was MK-8 (H4), and the major fatty acids (>10 %) were iso-C16 : 0 and anteiso-C14 : 0.
29927366	11	63	theme	major	1032:1036	arg1	%					1055:1055	>10 %	1051:1055	>10 %	1051:1055	The major isoprenoid quinone was MK-8 (H4), and the major fatty acids (>10 %) were iso-C16 : 0 and anteiso-C14 : 0.
29927366	11	63	theme	major	1032:1036	arg1	iso-C16 					1063:1070	iso-C16 	1063:1070	iso-C16 	1063:1070	The major isoprenoid quinone was MK-8 (H4), and the major fatty acids (>10 %) were iso-C16 : 0 and anteiso-C14 : 0.
29927366	8	64	theme	DNA	783:785	arg1	G+C content					787:797	The DNA G+C content	779:797	The DNA G+C content	779:797	The DNA G+C content was determined to be 69.9 mol%.
29927366	8	64	theme	DNA	783:785	arg1	%					828:828	69.9 mol%	820:828	69.9 mol%	820:828	The DNA G+C content was determined to be 69.9 mol%.
29927366	2	65	theme	soil	161:164	arg1	sample					166:171	a soil sample	159:171	a soil sample collected from a karst cave in Luoyang, Henan Province, China	159:233	A novel actinobacterium, designated strain CFH 30205T, was isolated from a soil sample collected from a karst cave in Luoyang, Henan Province, China.
29927366	7	66	theme	MSL-01T	761:767	arg1	strains					664:670	the type strains	655:670	the type strains of Nocardioides terrigena DS-17T (97.6 % sequence similarity) and Nocardioides sediminis MSL-01T (97.0 %)	655:776	On the basis of 16S rRNA gene sequence analysis, strain CFH 30205T was most closely related to the type strains of Nocardioides terrigena DS-17T (97.6 % sequence similarity) and Nocardioides sediminis MSL-01T (97.0 %).
29927366	13	67	theme	genotypic	1252:1260	arg1	data					1279:1282	phenotypic, genotypic and phylogenetic data	1240:1282	phenotypic, genotypic and phylogenetic data	1240:1282	On the basis of phenotypic, genotypic and phylogenetic data, strain CFH 30205T merits representation of a novel species of the genus Nocardioides, for which the name Nocardioides allogilvus sp.
29927366	9	68	theme	cell-wall	897:905	arg1	peptidoglycan					907:919	the cell-wall peptidoglycan	893:919	the cell-wall peptidoglycan	893:919	ll-2,6-Diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan.
29927366	1	69	theme	novel	36:40	arg1	actinobacterium					42:56	a novel actinobacterium	34:56	a novel actinobacterium	34:56	nov., a novel actinobacterium isolated from a karst cave.
29927366	1	69	theme	novel	36:40	arg1	nov.					28:31	nov.	28:31	nov.	28:31	nov., a novel actinobacterium isolated from a karst cave.
29927366	8	70	theme	69.9 mol	820:827	arg1	G+C content					787:797	The DNA G+C content	779:797	The DNA G+C content	779:797	The DNA G+C content was determined to be 69.9 mol%.
29927366	8	70	theme	69.9 mol	820:827	arg1	%					828:828	69.9 mol%	820:828	69.9 mol%	820:828	The DNA G+C content was determined to be 69.9 mol%.
29927366	6	71	theme	NaCl	548:551	arg1	presence					524:531	the presence	520:531	the presence of up to 1.5 % NaCl (w/v)	520:557	Strain CFH 30205T grew optimally at 28 °C, pH 9.0 and in the presence of up to 1.5 % NaCl (w/v).
29580368	11	0	theme	novel	1646:1650	arg1	species					1652:1658	a novel species	1644:1658	a novel species	1644:1658	On the basis of the consensus of phylogenetic and phenotypic analyses, this strain represents a novel species of the genus Anaerobacillus, for which the name Anaerobacillus isosaccharinicus sp.
29580368	9	1	dep	relatedness	1184:1194	arg1	%					1182:1182	%	1182:1182	%	1182:1182	In silico DNA-DNA hybridization with the four known species of the genus Anaerobacillus showed 21.8, 21.9, 22.4, and 21.5 % relatedness to Anaerobacillusarseniciselenatis DSM 15340T, Anaerobacilus alkalidiazotrophicus DSM 22531T, Anaerobacillusalkalilacustris DSM 18345T, and Anaerobacillus macyae DSM 16346T, respectively.
29580368	9	2	dep	Anaerobacilus	1243:1255	arg1	alkalidiazotrophicus					1257:1276	alkalidiazotrophicus	1257:1276	alkalidiazotrophicus	1257:1276	In silico DNA-DNA hybridization with the four known species of the genus Anaerobacillus showed 21.8, 21.9, 22.4, and 21.5 % relatedness to Anaerobacillusarseniciselenatis DSM 15340T, Anaerobacilus alkalidiazotrophicus DSM 22531T, Anaerobacillusalkalilacustris DSM 18345T, and Anaerobacillus macyae DSM 16346T, respectively.
29580368	7	3	theme	fatty	848:852	arg1	C16 					865:868	C16 	865:868	C16 	865:868	The predominant cellular fatty acids were C16 : 0, C16 : 1ω11c, anteiso-C15 : 0, iso-C15 : 0, C16 : 1ω7c/iso-C15 : 0 2-OH and C14 : 0.
29580368	7	3	theme	fatty	848:852	arg1	acids					854:858	The predominant cellular fatty acids	823:858	The predominant cellular fatty acids	823:858	The predominant cellular fatty acids were C16 : 0, C16 : 1ω11c, anteiso-C15 : 0, iso-C15 : 0, C16 : 1ω7c/iso-C15 : 0 2-OH and C14 : 0.
29580368	7	4	dep	C16 	865:868	arg1	 1ω11c					879:884	 1ω11c	879:884	 1ω11c	879:884	The predominant cellular fatty acids were C16 : 0, C16 : 1ω11c, anteiso-C15 : 0, iso-C15 : 0, C16 : 1ω7c/iso-C15 : 0 2-OH and C14 : 0.
29580368	7	4	dep	C16 	865:868	arg1	 0					913:914	 0	913:914	 0	913:914	The predominant cellular fatty acids were C16 : 0, C16 : 1ω11c, anteiso-C15 : 0, iso-C15 : 0, C16 : 1ω7c/iso-C15 : 0 2-OH and C14 : 0.
29580368	8	5	theme	DNA	1029:1031	arg1	G+C content					1033:1043	the DNA G+C content	1025:1043	the DNA G+C content	1025:1043	The cell wall peptidoglycan contained meso-diaminopimelic acid and the DNA G+C content was 37.7 mol%.
29580368	8	5	theme	DNA	1029:1031	arg1	%					1057:1057	37.7 mol%	1049:1057	37.7 mol%	1049:1057	The cell wall peptidoglycan contained meso-diaminopimelic acid and the DNA G+C content was 37.7 mol%.
29580368	11	6	dep	Anaerobacillus	1708:1721	arg1	isosaccharinicus					1723:1738	isosaccharinicus	1723:1738	isosaccharinicus	1723:1738	On the basis of the consensus of phylogenetic and phenotypic analyses, this strain represents a novel species of the genus Anaerobacillus, for which the name Anaerobacillus isosaccharinicus sp.
29580368	5	7	theme	6 	652:653	arg1	%					654:654	%	654:654	%	654:654	It was an aerotolerant anaerobe and an obligate alkaliphile that grew at pH 8.5-11, could tolerate up to 6 % (w/v) NaCl, and grew at a temperature between 10 and 40 °C.
29580368	4	8	theme	phenotypic	413:422	arg1	characterisation					438:453	phenotypic and metabolic characterisation	413:453	phenotypic and metabolic characterisation that showed that NB2006T was rod-shaped, Gram-stain-positive, motile and formed endospores	413:544	This was supported by phenotypic and metabolic characterisation that showed that NB2006T was rod-shaped, Gram-stain-positive, motile and formed endospores.
29580368	2	9	attach	isolated	123:130	arg2	NB2006T					111:117	Strain NB2006T	104:117	Strain NB2006T	104:117	Strain NB2006T was isolated from an isosaccharinate-degrading, nitrate-reducing enrichment culture in minimal freshwater medium at pH 10.
29580368	2	9	attach	isolated	123:130	arg1	culture					195:201	an isosaccharinate-degrading, nitrate-reducing enrichment culture	137:201	an isosaccharinate-degrading, nitrate-reducing enrichment culture in minimal freshwater medium at pH 10	137:239	Strain NB2006T was isolated from an isosaccharinate-degrading, nitrate-reducing enrichment culture in minimal freshwater medium at pH 10.
29580368	3	10	theme	genus	369:373	arg1	Anaerobacillus					375:388	the newly established genus Anaerobacillus	347:388	the newly established genus Anaerobacillus	347:388	Analysis of the 16S rRNA gene sequence indicated that this strain was most closely related to species of the newly established genus Anaerobacillus.
29580368	10	11	theme	species	1423:1429	arg1	strains					1406:1412	strains	1406:1412	strains of other species of the genus Anaerobacillus	1406:1457	NB2006T differed from strains of other species of the genus Anaerobacillus in its ability to metabolise isosaccharinate, an alkaline hydrolysis product of cellulose.
29580368	6	12	theme	substrates	766:775	arg1	number					748:753	a number	746:753	a number of organic substrates	746:775	In addition, it could utilise a number of organic substrates, and was able to reduce nitrate and arsenate.
29580368	7	13	theme	predominant	827:837	arg1	C16 					865:868	C16 	865:868	C16 	865:868	The predominant cellular fatty acids were C16 : 0, C16 : 1ω11c, anteiso-C15 : 0, iso-C15 : 0, C16 : 1ω7c/iso-C15 : 0 2-OH and C14 : 0.
29580368	7	13	theme	predominant	827:837	arg1	acids					854:858	The predominant cellular fatty acids	823:858	The predominant cellular fatty acids	823:858	The predominant cellular fatty acids were C16 : 0, C16 : 1ω11c, anteiso-C15 : 0, iso-C15 : 0, C16 : 1ω7c/iso-C15 : 0 2-OH and C14 : 0.
29580368	11	14	theme	Anaerobacillus	1673:1686	arg1	species					1652:1658	a novel species	1644:1658	a novel species	1644:1658	On the basis of the consensus of phylogenetic and phenotypic analyses, this strain represents a novel species of the genus Anaerobacillus, for which the name Anaerobacillus isosaccharinicus sp.
29580368	3	15	theme	Anaerobacillus	375:388	arg1	species					336:342	species	336:342	species of the newly established genus Anaerobacillus	336:388	Analysis of the 16S rRNA gene sequence indicated that this strain was most closely related to species of the newly established genus Anaerobacillus.
29580368	2	16	theme	enrichment	184:193	arg1	culture					195:201	an isosaccharinate-degrading, nitrate-reducing enrichment culture	137:201	an isosaccharinate-degrading, nitrate-reducing enrichment culture in minimal freshwater medium at pH 10	137:239	Strain NB2006T was isolated from an isosaccharinate-degrading, nitrate-reducing enrichment culture in minimal freshwater medium at pH 10.
29580368	4	17	theme	metabolic	428:436	arg1	characterisation					438:453	phenotypic and metabolic characterisation	413:453	phenotypic and metabolic characterisation that showed that NB2006T was rod-shaped, Gram-stain-positive, motile and formed endospores	413:544	This was supported by phenotypic and metabolic characterisation that showed that NB2006T was rod-shaped, Gram-stain-positive, motile and formed endospores.
29580368	9	18	theme	Anaerobacilus	1243:1255	arg1	22531T					1282:1287	Anaerobacilus alkalidiazotrophicus DSM 22531T	1243:1287	Anaerobacilus alkalidiazotrophicus DSM 22531T	1243:1287	In silico DNA-DNA hybridization with the four known species of the genus Anaerobacillus showed 21.8, 21.9, 22.4, and 21.5 % relatedness to Anaerobacillusarseniciselenatis DSM 15340T, Anaerobacilus alkalidiazotrophicus DSM 22531T, Anaerobacillusalkalilacustris DSM 18345T, and Anaerobacillus macyae DSM 16346T, respectively.
29580368	9	19	theme	Anaerobacillus	1336:1349	arg1	16346T					1362:1367	Anaerobacillus macyae DSM 16346T	1336:1367	Anaerobacillus macyae DSM 16346T	1336:1367	In silico DNA-DNA hybridization with the four known species of the genus Anaerobacillus showed 21.8, 21.9, 22.4, and 21.5 % relatedness to Anaerobacillusarseniciselenatis DSM 15340T, Anaerobacilus alkalidiazotrophicus DSM 22531T, Anaerobacillusalkalilacustris DSM 18345T, and Anaerobacillus macyae DSM 16346T, respectively.
29580368	3	20	theme	gene	267:270	arg1	sequence					272:279	the 16S rRNA gene sequence	254:279	the 16S rRNA gene sequence	254:279	Analysis of the 16S rRNA gene sequence indicated that this strain was most closely related to species of the newly established genus Anaerobacillus.
29580368	6	21	theme	organic	758:764	arg1	substrates					766:775	organic substrates	758:775	organic substrates	758:775	In addition, it could utilise a number of organic substrates, and was able to reduce nitrate and arsenate.
29580368	13	22	theme	100644T=LMG	1795:1805	arg1	30032T					1807:1812	=DSM 100644T=LMG 30032T	1790:1812	=DSM 100644T=LMG 30032T	1790:1812	The type strain is NB2006T (=DSM 100644T=LMG 30032T).
29580368	13	22	theme	100644T=LMG	1795:1805	arg1	NB2006T					1781:1787	NB2006T	1781:1787	NB2006T (=DSM 100644T=LMG 30032T)	1781:1813	The type strain is NB2006T (=DSM 100644T=LMG 30032T).
29580368	11	23	theme	phylogenetic	1583:1594	arg1	analyses					1611:1618	phylogenetic and phenotypic analyses	1583:1618	phylogenetic and phenotypic analyses	1583:1618	On the basis of the consensus of phylogenetic and phenotypic analyses, this strain represents a novel species of the genus Anaerobacillus, for which the name Anaerobacillus isosaccharinicus sp.
29580368	2	24	from	pH	235:236	arg1	culture					195:201	an isosaccharinate-degrading, nitrate-reducing enrichment culture	137:201	an isosaccharinate-degrading, nitrate-reducing enrichment culture in minimal freshwater medium at pH 10	137:239	Strain NB2006T was isolated from an isosaccharinate-degrading, nitrate-reducing enrichment culture in minimal freshwater medium at pH 10.
29580368	2	24	from	pH	235:236	arg1	medium					225:230	minimal freshwater medium	206:230	minimal freshwater medium at pH 10	206:239	Strain NB2006T was isolated from an isosaccharinate-degrading, nitrate-reducing enrichment culture in minimal freshwater medium at pH 10.
29580368	11	25	theme	consensus	1570:1578	arg1	basis					1557:1561	the basis	1553:1561	the basis of the consensus of phylogenetic and phenotypic analyses	1553:1618	On the basis of the consensus of phylogenetic and phenotypic analyses, this strain represents a novel species of the genus Anaerobacillus, for which the name Anaerobacillus isosaccharinicus sp.
29580368	3	26	theme	sequence	272:279	arg1	Analysis					242:249	Analysis	242:249	Analysis of the 16S rRNA gene sequence	242:279	Analysis of the 16S rRNA gene sequence indicated that this strain was most closely related to species of the newly established genus Anaerobacillus.
29580368	11	27	theme	analyses	1611:1618	arg1	consensus					1570:1578	the consensus	1566:1578	the consensus of phylogenetic and phenotypic analyses	1566:1618	On the basis of the consensus of phylogenetic and phenotypic analyses, this strain represents a novel species of the genus Anaerobacillus, for which the name Anaerobacillus isosaccharinicus sp.
29580368	1	28	theme	isosaccharinic	83:96	arg1	acid					98:101	isosaccharinic acid	83:101	isosaccharinic acid	83:101	nov., an alkaliphilic bacterium which degrades isosaccharinic acid.
29580368	7	29	dep	C16 	917:920	arg1	 1ω7c/iso-C15 					922:935	 1ω7c/iso-C15 	922:935	 1ω7c/iso-C15 	922:935	The predominant cellular fatty acids were C16 : 0, C16 : 1ω11c, anteiso-C15 : 0, iso-C15 : 0, C16 : 1ω7c/iso-C15 : 0 2-OH and C14 : 0.
29580368	7	29	dep	C16 	917:920	arg1	2-OH					940:943	 0 2-OH	937:943	C16 : 1ω7c/iso-C15 : 0 2-OH	917:943	The predominant cellular fatty acids were C16 : 0, C16 : 1ω11c, anteiso-C15 : 0, iso-C15 : 0, C16 : 1ω7c/iso-C15 : 0 2-OH and C14 : 0.
29580368	9	30	theme	DSM	1358:1360	arg1	16346T					1362:1367	Anaerobacillus macyae DSM 16346T	1336:1367	Anaerobacillus macyae DSM 16346T	1336:1367	In silico DNA-DNA hybridization with the four known species of the genus Anaerobacillus showed 21.8, 21.9, 22.4, and 21.5 % relatedness to Anaerobacillusarseniciselenatis DSM 15340T, Anaerobacilus alkalidiazotrophicus DSM 22531T, Anaerobacillusalkalilacustris DSM 18345T, and Anaerobacillus macyae DSM 16346T, respectively.
29580368	0	31	theme	isosaccharinicus	15:30	arg1	sp					32:33	Anaerobacillus isosaccharinicus sp	0:33	Anaerobacillus isosaccharinicus sp.	0:34	Anaerobacillus isosaccharinicus sp.
29580368	2	32	theme	isosaccharinate-degrading	140:164	arg1	culture					195:201	an isosaccharinate-degrading, nitrate-reducing enrichment culture	137:201	an isosaccharinate-degrading, nitrate-reducing enrichment culture in minimal freshwater medium at pH 10	137:239	Strain NB2006T was isolated from an isosaccharinate-degrading, nitrate-reducing enrichment culture in minimal freshwater medium at pH 10.
29580368	3	33	theme	16S	258:260	arg1	rRNA					262:265	16S rRNA	258:265	the 16S rRNA gene sequence	254:279	Analysis of the 16S rRNA gene sequence indicated that this strain was most closely related to species of the newly established genus Anaerobacillus.
29580368	0	34	theme	Anaerobacillus	0:13	arg1	sp					32:33	Anaerobacillus isosaccharinicus sp	0:33	Anaerobacillus isosaccharinicus sp.	0:34	Anaerobacillus isosaccharinicus sp.
29580368	11	35	theme	Anaerobacillus	1708:1721	arg1	sp					1740:1741	the name Anaerobacillus isosaccharinicus sp	1699:1741	the name Anaerobacillus isosaccharinicus sp	1699:1741	On the basis of the consensus of phylogenetic and phenotypic analyses, this strain represents a novel species of the genus Anaerobacillus, for which the name Anaerobacillus isosaccharinicus sp.
29580368	3	36	theme	rRNA	262:265	arg1	sequence					272:279	the 16S rRNA gene sequence	254:279	the 16S rRNA gene sequence	254:279	Analysis of the 16S rRNA gene sequence indicated that this strain was most closely related to species of the newly established genus Anaerobacillus.
29580368	10	37	theme	alkaline	1508:1515	arg1	product					1528:1534	an alkaline hydrolysis product	1505:1534	an alkaline hydrolysis product of cellulose	1505:1547	NB2006T differed from strains of other species of the genus Anaerobacillus in its ability to metabolise isosaccharinate, an alkaline hydrolysis product of cellulose.
29580368	10	37	theme	alkaline	1508:1515	arg1	isosaccharinate					1488:1502	isosaccharinate	1488:1502	isosaccharinate	1488:1502	NB2006T differed from strains of other species of the genus Anaerobacillus in its ability to metabolise isosaccharinate, an alkaline hydrolysis product of cellulose.
29580368	2	38	dep	isosaccharinate-degrading	140:164	arg1	nitrate-reducing					167:182	nitrate-reducing	167:182	nitrate-reducing	167:182	Strain NB2006T was isolated from an isosaccharinate-degrading, nitrate-reducing enrichment culture in minimal freshwater medium at pH 10.
29580368	10	39	theme	other	1417:1421	arg1	species					1423:1429	other species	1417:1429	other species of the genus Anaerobacillus	1417:1457	NB2006T differed from strains of other species of the genus Anaerobacillus in its ability to metabolise isosaccharinate, an alkaline hydrolysis product of cellulose.
29580368	9	40	theme	known	1106:1110	arg1	species					1112:1118	the four known species	1097:1118	the four known species of the genus Anaerobacillus	1097:1146	In silico DNA-DNA hybridization with the four known species of the genus Anaerobacillus showed 21.8, 21.9, 22.4, and 21.5 % relatedness to Anaerobacillusarseniciselenatis DSM 15340T, Anaerobacilus alkalidiazotrophicus DSM 22531T, Anaerobacillusalkalilacustris DSM 18345T, and Anaerobacillus macyae DSM 16346T, respectively.
29580368	9	41	theme	In	1060:1061	arg1	hybridization					1078:1090	In silico DNA-DNA hybridization	1060:1090	In silico DNA-DNA hybridization with the four known species of the genus Anaerobacillus	1060:1146	In silico DNA-DNA hybridization with the four known species of the genus Anaerobacillus showed 21.8, 21.9, 22.4, and 21.5 % relatedness to Anaerobacillusarseniciselenatis DSM 15340T, Anaerobacilus alkalidiazotrophicus DSM 22531T, Anaerobacillusalkalilacustris DSM 18345T, and Anaerobacillus macyae DSM 16346T, respectively.
29580368	9	42	theme	DSM	1231:1233	arg1	15340T					1235:1240	Anaerobacillusarseniciselenatis DSM 15340T	1199:1240	Anaerobacillusarseniciselenatis DSM 15340T	1199:1240	In silico DNA-DNA hybridization with the four known species of the genus Anaerobacillus showed 21.8, 21.9, 22.4, and 21.5 % relatedness to Anaerobacillusarseniciselenatis DSM 15340T, Anaerobacilus alkalidiazotrophicus DSM 22531T, Anaerobacillusalkalilacustris DSM 18345T, and Anaerobacillus macyae DSM 16346T, respectively.
29580368	2	43	theme	freshwater	214:223	arg1	medium					225:230	minimal freshwater medium	206:230	minimal freshwater medium at pH 10	206:239	Strain NB2006T was isolated from an isosaccharinate-degrading, nitrate-reducing enrichment culture in minimal freshwater medium at pH 10.
29580368	10	44	theme	Anaerobacillus	1444:1457	arg1	species					1423:1429	other species	1417:1429	other species of the genus Anaerobacillus	1417:1457	NB2006T differed from strains of other species of the genus Anaerobacillus in its ability to metabolise isosaccharinate, an alkaline hydrolysis product of cellulose.
29580368	11	45	theme	name	1703:1706	arg1	sp					1740:1741	the name Anaerobacillus isosaccharinicus sp	1699:1741	the name Anaerobacillus isosaccharinicus sp	1699:1741	On the basis of the consensus of phylogenetic and phenotypic analyses, this strain represents a novel species of the genus Anaerobacillus, for which the name Anaerobacillus isosaccharinicus sp.
29580368	8	46	theme	wall	967:970	arg1	peptidoglycan					972:984	The cell wall peptidoglycan	958:984	The cell wall peptidoglycan	958:984	The cell wall peptidoglycan contained meso-diaminopimelic acid and the DNA G+C content was 37.7 mol%.
29580368	2	47	theme	minimal	206:212	arg1	medium					225:230	minimal freshwater medium	206:230	minimal freshwater medium at pH 10	206:239	Strain NB2006T was isolated from an isosaccharinate-degrading, nitrate-reducing enrichment culture in minimal freshwater medium at pH 10.
29580368	10	48	theme	genus	1438:1442	arg1	Anaerobacillus					1444:1457	the genus Anaerobacillus	1434:1457	the genus Anaerobacillus	1434:1457	NB2006T differed from strains of other species of the genus Anaerobacillus in its ability to metabolise isosaccharinate, an alkaline hydrolysis product of cellulose.
29580368	13	49	theme	type	1766:1769	arg1	NB2006T					1781:1787	NB2006T	1781:1787	NB2006T (=DSM 100644T=LMG 30032T)	1781:1813	The type strain is NB2006T (=DSM 100644T=LMG 30032T).
29580368	13	49	theme	type	1766:1769	arg1	strain					1771:1776	The type strain	1762:1776	The type strain	1762:1776	The type strain is NB2006T (=DSM 100644T=LMG 30032T).
29580368	5	50	dep	%	654:654	arg1	w/v					657:659	w/v	657:659	w/v	657:659	It was an aerotolerant anaerobe and an obligate alkaliphile that grew at pH 8.5-11, could tolerate up to 6 % (w/v) NaCl, and grew at a temperature between 10 and 40 °C.
29580368	9	51	dep	%	1182:1182	arg1	22.4					1167:1170	22.4	1167:1170	22.4	1167:1170	In silico DNA-DNA hybridization with the four known species of the genus Anaerobacillus showed 21.8, 21.9, 22.4, and 21.5 % relatedness to Anaerobacillusarseniciselenatis DSM 15340T, Anaerobacilus alkalidiazotrophicus DSM 22531T, Anaerobacillusalkalilacustris DSM 18345T, and Anaerobacillus macyae DSM 16346T, respectively.
29580368	9	51	dep	%	1182:1182	arg1	21.5 					1177:1181	21.5 	1177:1181	21.5 	1177:1181	In silico DNA-DNA hybridization with the four known species of the genus Anaerobacillus showed 21.8, 21.9, 22.4, and 21.5 % relatedness to Anaerobacillusarseniciselenatis DSM 15340T, Anaerobacilus alkalidiazotrophicus DSM 22531T, Anaerobacillusalkalilacustris DSM 18345T, and Anaerobacillus macyae DSM 16346T, respectively.
29580368	8	52	theme	cell	962:965	arg1	peptidoglycan					972:984	The cell wall peptidoglycan	958:984	The cell wall peptidoglycan	958:984	The cell wall peptidoglycan contained meso-diaminopimelic acid and the DNA G+C content was 37.7 mol%.
29580368	11	53	theme	phenotypic	1600:1609	arg1	analyses					1611:1618	phylogenetic and phenotypic analyses	1583:1618	phylogenetic and phenotypic analyses	1583:1618	On the basis of the consensus of phylogenetic and phenotypic analyses, this strain represents a novel species of the genus Anaerobacillus, for which the name Anaerobacillus isosaccharinicus sp.
29580368	3	54	theme	established	357:367	arg1	Anaerobacillus					375:388	the newly established genus Anaerobacillus	347:388	the newly established genus Anaerobacillus	347:388	Analysis of the 16S rRNA gene sequence indicated that this strain was most closely related to species of the newly established genus Anaerobacillus.
29580368	10	55	theme	cellulose	1539:1547	arg1	product					1528:1534	an alkaline hydrolysis product	1505:1534	an alkaline hydrolysis product of cellulose	1505:1547	NB2006T differed from strains of other species of the genus Anaerobacillus in its ability to metabolise isosaccharinate, an alkaline hydrolysis product of cellulose.
29580368	10	55	theme	cellulose	1539:1547	arg1	isosaccharinate					1488:1502	isosaccharinate	1488:1502	isosaccharinate	1488:1502	NB2006T differed from strains of other species of the genus Anaerobacillus in its ability to metabolise isosaccharinate, an alkaline hydrolysis product of cellulose.
29580368	2	56	theme	Strain	104:109	arg1	NB2006T					111:117	Strain NB2006T	104:117	Strain NB2006T	104:117	Strain NB2006T was isolated from an isosaccharinate-degrading, nitrate-reducing enrichment culture in minimal freshwater medium at pH 10.
29580368	9	57	theme	DNA-DNA	1070:1076	arg1	hybridization					1078:1090	In silico DNA-DNA hybridization	1060:1090	In silico DNA-DNA hybridization with the four known species of the genus Anaerobacillus	1060:1146	In silico DNA-DNA hybridization with the four known species of the genus Anaerobacillus showed 21.8, 21.9, 22.4, and 21.5 % relatedness to Anaerobacillusarseniciselenatis DSM 15340T, Anaerobacilus alkalidiazotrophicus DSM 22531T, Anaerobacillusalkalilacustris DSM 18345T, and Anaerobacillus macyae DSM 16346T, respectively.
29580368	9	58	theme	Anaerobacillusarseniciselenatis	1199:1229	arg1	15340T					1235:1240	Anaerobacillusarseniciselenatis DSM 15340T	1199:1240	Anaerobacillusarseniciselenatis DSM 15340T	1199:1240	In silico DNA-DNA hybridization with the four known species of the genus Anaerobacillus showed 21.8, 21.9, 22.4, and 21.5 % relatedness to Anaerobacillusarseniciselenatis DSM 15340T, Anaerobacilus alkalidiazotrophicus DSM 22531T, Anaerobacillusalkalilacustris DSM 18345T, and Anaerobacillus macyae DSM 16346T, respectively.
29580368	9	59	theme	Anaerobacillus	1133:1146	arg1	species					1112:1118	the four known species	1097:1118	the four known species of the genus Anaerobacillus	1097:1146	In silico DNA-DNA hybridization with the four known species of the genus Anaerobacillus showed 21.8, 21.9, 22.4, and 21.5 % relatedness to Anaerobacillusarseniciselenatis DSM 15340T, Anaerobacilus alkalidiazotrophicus DSM 22531T, Anaerobacillusalkalilacustris DSM 18345T, and Anaerobacillus macyae DSM 16346T, respectively.
29580368	9	60	theme	genus	1127:1131	arg1	Anaerobacillus					1133:1146	the genus Anaerobacillus	1123:1146	the genus Anaerobacillus	1123:1146	In silico DNA-DNA hybridization with the four known species of the genus Anaerobacillus showed 21.8, 21.9, 22.4, and 21.5 % relatedness to Anaerobacillusarseniciselenatis DSM 15340T, Anaerobacilus alkalidiazotrophicus DSM 22531T, Anaerobacillusalkalilacustris DSM 18345T, and Anaerobacillus macyae DSM 16346T, respectively.
29580368	5	61	theme	obligate	586:593	arg1	alkaliphile					595:605	an obligate alkaliphile	583:605	an obligate alkaliphile	583:605	It was an aerotolerant anaerobe and an obligate alkaliphile that grew at pH 8.5-11, could tolerate up to 6 % (w/v) NaCl, and grew at a temperature between 10 and 40 °C.
29580368	5	62	theme	%	654:654	arg1	NaCl					662:665	6 % (w/v) NaCl	652:665	6 % (w/v) NaCl	652:665	It was an aerotolerant anaerobe and an obligate alkaliphile that grew at pH 8.5-11, could tolerate up to 6 % (w/v) NaCl, and grew at a temperature between 10 and 40 °C.
29580368	10	63	theme	hydrolysis	1517:1526	arg1	product					1528:1534	an alkaline hydrolysis product	1505:1534	an alkaline hydrolysis product of cellulose	1505:1547	NB2006T differed from strains of other species of the genus Anaerobacillus in its ability to metabolise isosaccharinate, an alkaline hydrolysis product of cellulose.
29580368	10	63	theme	hydrolysis	1517:1526	arg1	isosaccharinate					1488:1502	isosaccharinate	1488:1502	isosaccharinate	1488:1502	NB2006T differed from strains of other species of the genus Anaerobacillus in its ability to metabolise isosaccharinate, an alkaline hydrolysis product of cellulose.
29580368	9	64	theme	DSM	1278:1280	arg1	22531T					1282:1287	Anaerobacilus alkalidiazotrophicus DSM 22531T	1243:1287	Anaerobacilus alkalidiazotrophicus DSM 22531T	1243:1287	In silico DNA-DNA hybridization with the four known species of the genus Anaerobacillus showed 21.8, 21.9, 22.4, and 21.5 % relatedness to Anaerobacillusarseniciselenatis DSM 15340T, Anaerobacilus alkalidiazotrophicus DSM 22531T, Anaerobacillusalkalilacustris DSM 18345T, and Anaerobacillus macyae DSM 16346T, respectively.
29580368	9	65	dep	Anaerobacillus	1336:1349	arg1	macyae					1351:1356	macyae	1351:1356	macyae	1351:1356	In silico DNA-DNA hybridization with the four known species of the genus Anaerobacillus showed 21.8, 21.9, 22.4, and 21.5 % relatedness to Anaerobacillusarseniciselenatis DSM 15340T, Anaerobacilus alkalidiazotrophicus DSM 22531T, Anaerobacillusalkalilacustris DSM 18345T, and Anaerobacillus macyae DSM 16346T, respectively.
29580368	8	66	theme	37.7 mol	1049:1056	arg1	G+C content					1033:1043	the DNA G+C content	1025:1043	the DNA G+C content	1025:1043	The cell wall peptidoglycan contained meso-diaminopimelic acid and the DNA G+C content was 37.7 mol%.
29580368	8	66	theme	37.7 mol	1049:1056	arg1	%					1057:1057	37.7 mol%	1049:1057	37.7 mol%	1049:1057	The cell wall peptidoglycan contained meso-diaminopimelic acid and the DNA G+C content was 37.7 mol%.
29580368	8	67	theme	meso-diaminopimelic	996:1014	arg1	acid					1016:1019	meso-diaminopimelic acid	996:1019	meso-diaminopimelic acid	996:1019	The cell wall peptidoglycan contained meso-diaminopimelic acid and the DNA G+C content was 37.7 mol%.
29580368	8	68	contain	contained	986:994	arg2	acid					1016:1019	meso-diaminopimelic acid	996:1019	meso-diaminopimelic acid	996:1019	The cell wall peptidoglycan contained meso-diaminopimelic acid and the DNA G+C content was 37.7 mol%.
29580368	8	68	contain	contained	986:994	arg1	peptidoglycan					972:984	The cell wall peptidoglycan	958:984	The cell wall peptidoglycan	958:984	The cell wall peptidoglycan contained meso-diaminopimelic acid and the DNA G+C content was 37.7 mol%.
29580368	9	69	dep	In	1060:1061	arg1	silico					1063:1068	silico	1063:1068	silico	1063:1068	In silico DNA-DNA hybridization with the four known species of the genus Anaerobacillus showed 21.8, 21.9, 22.4, and 21.5 % relatedness to Anaerobacillusarseniciselenatis DSM 15340T, Anaerobacilus alkalidiazotrophicus DSM 22531T, Anaerobacillusalkalilacustris DSM 18345T, and Anaerobacillus macyae DSM 16346T, respectively.
29580368	11	70	theme	genus	1667:1671	arg1	Anaerobacillus					1673:1686	the genus Anaerobacillus	1663:1686	the genus Anaerobacillus	1663:1686	On the basis of the consensus of phylogenetic and phenotypic analyses, this strain represents a novel species of the genus Anaerobacillus, for which the name Anaerobacillus isosaccharinicus sp.
29580368	13	71	theme	=DSM	1790:1793	arg1	30032T					1807:1812	=DSM 100644T=LMG 30032T	1790:1812	=DSM 100644T=LMG 30032T	1790:1812	The type strain is NB2006T (=DSM 100644T=LMG 30032T).
29580368	13	71	theme	=DSM	1790:1793	arg1	NB2006T					1781:1787	NB2006T	1781:1787	NB2006T (=DSM 100644T=LMG 30032T)	1781:1813	The type strain is NB2006T (=DSM 100644T=LMG 30032T).
29580368	9	72	theme	Anaerobacillusalkalilacustris	1290:1318	arg1	18345T					1324:1329	Anaerobacillusalkalilacustris DSM 18345T	1290:1329	Anaerobacillusalkalilacustris DSM 18345T	1290:1329	In silico DNA-DNA hybridization with the four known species of the genus Anaerobacillus showed 21.8, 21.9, 22.4, and 21.5 % relatedness to Anaerobacillusarseniciselenatis DSM 15340T, Anaerobacilus alkalidiazotrophicus DSM 22531T, Anaerobacillusalkalilacustris DSM 18345T, and Anaerobacillus macyae DSM 16346T, respectively.
29580368	2	73	from	culture	195:201	arg1	pH					235:236	pH 10	235:239	pH 10	235:239	Strain NB2006T was isolated from an isosaccharinate-degrading, nitrate-reducing enrichment culture in minimal freshwater medium at pH 10.
29580368	2	73	from	culture	195:201	arg1	medium					225:230	minimal freshwater medium	206:230	minimal freshwater medium at pH 10	206:239	Strain NB2006T was isolated from an isosaccharinate-degrading, nitrate-reducing enrichment culture in minimal freshwater medium at pH 10.
29580368	9	74	with	hybridization	1078:1090	arg1	species					1112:1118	the four known species	1097:1118	the four known species of the genus Anaerobacillus	1097:1146	In silico DNA-DNA hybridization with the four known species of the genus Anaerobacillus showed 21.8, 21.9, 22.4, and 21.5 % relatedness to Anaerobacillusarseniciselenatis DSM 15340T, Anaerobacilus alkalidiazotrophicus DSM 22531T, Anaerobacillusalkalilacustris DSM 18345T, and Anaerobacillus macyae DSM 16346T, respectively.
29580368	1	75	theme	alkaliphilic	45:56	arg1	bacterium					58:66	an alkaliphilic bacterium	42:66	an alkaliphilic bacterium which degrades isosaccharinic acid	42:101	nov., an alkaliphilic bacterium which degrades isosaccharinic acid.
29580368	1	75	theme	alkaliphilic	45:56	arg1	nov.					36:39	nov.	36:39	nov.	36:39	nov., an alkaliphilic bacterium which degrades isosaccharinic acid.
29580368	5	76	theme	aerotolerant	557:568	arg1	anaerobe					570:577	an aerotolerant anaerobe	554:577	an aerotolerant anaerobe	554:577	It was an aerotolerant anaerobe and an obligate alkaliphile that grew at pH 8.5-11, could tolerate up to 6 % (w/v) NaCl, and grew at a temperature between 10 and 40 °C.
29580368	5	76	theme	aerotolerant	557:568	arg1	It					547:548	It	547:548	It	547:548	It was an aerotolerant anaerobe and an obligate alkaliphile that grew at pH 8.5-11, could tolerate up to 6 % (w/v) NaCl, and grew at a temperature between 10 and 40 °C.
29580368	7	77	theme	cellular	839:846	arg1	C16 					865:868	C16 	865:868	C16 	865:868	The predominant cellular fatty acids were C16 : 0, C16 : 1ω11c, anteiso-C15 : 0, iso-C15 : 0, C16 : 1ω7c/iso-C15 : 0 2-OH and C14 : 0.
29580368	7	77	theme	cellular	839:846	arg1	acids					854:858	The predominant cellular fatty acids	823:858	The predominant cellular fatty acids	823:858	The predominant cellular fatty acids were C16 : 0, C16 : 1ω11c, anteiso-C15 : 0, iso-C15 : 0, C16 : 1ω7c/iso-C15 : 0 2-OH and C14 : 0.
29580368	9	78	theme	DSM	1320:1322	arg1	18345T					1324:1329	Anaerobacillusalkalilacustris DSM 18345T	1290:1329	Anaerobacillusalkalilacustris DSM 18345T	1290:1329	In silico DNA-DNA hybridization with the four known species of the genus Anaerobacillus showed 21.8, 21.9, 22.4, and 21.5 % relatedness to Anaerobacillusarseniciselenatis DSM 15340T, Anaerobacilus alkalidiazotrophicus DSM 22531T, Anaerobacillusalkalilacustris DSM 18345T, and Anaerobacillus macyae DSM 16346T, respectively.
30969170	3	0	theme	crosslinked	759:769	arg1	fragments					794:802	crosslinked Lys-type peptidoglycan fragments	759:802	crosslinked Lys-type peptidoglycan fragments	759:802	Our structural and biochemical studies show that SagA is a NlpC/p60-endopeptidase that preferentially hydrolyzes crosslinked Lys-type peptidoglycan fragments.
30969170	2	1	theme	mammalian	629:637	arg1	cells					639:643	mammalian cells	629:643	mammalian cells	629:643	Here we show E. faecium has unique peptidoglycan composition and remodeling activity through SagA, which generates smaller muropeptides that more effectively activates nucleotide-binding oligomerization domain-containing protein 2 (NOD2) in mammalian cells.
30969170	5	2	theme	hydrolase	1026:1034	arg1	activity					1036:1043	hydrolase activity	1026:1043	hydrolase activity	1026:1043	Our results demonstrate that the peptidoglycan composition and hydrolase activity of specific microbiota species can activate host immune pathways and enhance tolerance to pathogens.
30969170	2	3	from	protein	609:615	arg1	cells					639:643	mammalian cells	629:643	mammalian cells	629:643	Here we show E. faecium has unique peptidoglycan composition and remodeling activity through SagA, which generates smaller muropeptides that more effectively activates nucleotide-binding oligomerization domain-containing protein 2 (NOD2) in mammalian cells.
30969170	0	4	theme	bacterial	98:106	arg1	pathogenesis					108:119	bacterial pathogenesis	98:119	bacterial pathogenesis	98:119	Enterococcus faecium secreted antigen A generates muropeptides to enhance host immunity and limit bacterial pathogenesis.
30969170	5	5	theme	immune	1094:1099	arg1	pathways					1101:1108	host immune pathways	1089:1108	host immune pathways	1089:1108	Our results demonstrate that the peptidoglycan composition and hydrolase activity of specific microbiota species can activate host immune pathways and enhance tolerance to pathogens.
30969170	5	6	dep	composition	1010:1020	arg1	the					992:994	the	992:994	the	992:994	Our results demonstrate that the peptidoglycan composition and hydrolase activity of specific microbiota species can activate host immune pathways and enhance tolerance to pathogens.
30969170	1	7	theme	precise	345:351	arg1	unknown					379:385	unknown	379:385	unknown	379:385	We discovered that Enterococcus faecium (E. faecium), a ubiquitous commensal bacterium, and its secreted peptidoglycan hydrolase (SagA) were sufficient to enhance intestinal barrier function and pathogen tolerance, but the precise biochemical mechanism was unknown.
30969170	1	7	theme	precise	345:351	arg1	mechanism					365:373	the precise biochemical mechanism	341:373	the precise biochemical mechanism	341:373	We discovered that Enterococcus faecium (E. faecium), a ubiquitous commensal bacterium, and its secreted peptidoglycan hydrolase (SagA) were sufficient to enhance intestinal barrier function and pathogen tolerance, but the precise biochemical mechanism was unknown.
30969170	5	8	theme	microbiota	1057:1066	arg1	species					1068:1074	specific microbiota species	1048:1074	specific microbiota species	1048:1074	Our results demonstrate that the peptidoglycan composition and hydrolase activity of specific microbiota species can activate host immune pathways and enhance tolerance to pathogens.
30969170	1	9	theme	biochemical	353:363	arg1	unknown					379:385	unknown	379:385	unknown	379:385	We discovered that Enterococcus faecium (E. faecium), a ubiquitous commensal bacterium, and its secreted peptidoglycan hydrolase (SagA) were sufficient to enhance intestinal barrier function and pathogen tolerance, but the precise biochemical mechanism was unknown.
30969170	1	9	theme	biochemical	353:363	arg1	mechanism					365:373	the precise biochemical mechanism	341:373	the precise biochemical mechanism	341:373	We discovered that Enterococcus faecium (E. faecium), a ubiquitous commensal bacterium, and its secreted peptidoglycan hydrolase (SagA) were sufficient to enhance intestinal barrier function and pathogen tolerance, but the precise biochemical mechanism was unknown.
30969170	3	10	theme	Lys-type	771:778	arg1	fragments					794:802	crosslinked Lys-type peptidoglycan fragments	759:802	crosslinked Lys-type peptidoglycan fragments	759:802	Our structural and biochemical studies show that SagA is a NlpC/p60-endopeptidase that preferentially hydrolyzes crosslinked Lys-type peptidoglycan fragments.
30969170	3	11	theme	peptidoglycan	780:792	arg1	fragments					794:802	crosslinked Lys-type peptidoglycan fragments	759:802	crosslinked Lys-type peptidoglycan fragments	759:802	Our structural and biochemical studies show that SagA is a NlpC/p60-endopeptidase that preferentially hydrolyzes crosslinked Lys-type peptidoglycan fragments.
30969170	0	12	theme	host	74:77	arg1	immunity					79:86	host immunity	74:86	host immunity	74:86	Enterococcus faecium secreted antigen A generates muropeptides to enhance host immunity and limit bacterial pathogenesis.
30969170	5	13	theme	species	1068:1074	arg1	composition					1010:1020	peptidoglycan composition	996:1020	peptidoglycan composition	996:1020	Our results demonstrate that the peptidoglycan composition and hydrolase activity of specific microbiota species can activate host immune pathways and enhance tolerance to pathogens.
30969170	5	13	theme	species	1068:1074	arg1	activity					1036:1043	hydrolase activity	1026:1043	hydrolase activity	1026:1043	Our results demonstrate that the peptidoglycan composition and hydrolase activity of specific microbiota species can activate host immune pathways and enhance tolerance to pathogens.
30969170	0	14	theme	Enterococcus	0:11	arg1	faecium					13:19	Enterococcus faecium	0:19	Enterococcus faecium secreted antigen A	0:38	Enterococcus faecium secreted antigen A generates muropeptides to enhance host immunity and limit bacterial pathogenesis.
30969170	4	15	theme	SagA	805:808	arg1	secretion					810:818	SagA secretion	805:818	SagA secretion	805:818	SagA secretion and NlpC/p60-endopeptidase activity was required for enhancing probiotic bacteria activity against Clostridium difficile pathogenesis in vivo.
30969170	1	16	theme	ubiquitous	178:187	arg1	bacterium					199:207	a ubiquitous commensal bacterium	176:207	a ubiquitous commensal bacterium	176:207	We discovered that Enterococcus faecium (E. faecium), a ubiquitous commensal bacterium, and its secreted peptidoglycan hydrolase (SagA) were sufficient to enhance intestinal barrier function and pathogen tolerance, but the precise biochemical mechanism was unknown.
30969170	1	16	theme	ubiquitous	178:187	arg1	sufficient					263:272	sufficient	263:272	sufficient	263:272	We discovered that Enterococcus faecium (E. faecium), a ubiquitous commensal bacterium, and its secreted peptidoglycan hydrolase (SagA) were sufficient to enhance intestinal barrier function and pathogen tolerance, but the precise biochemical mechanism was unknown.
30969170	0	17	theme	antigen	30:36	arg1	A					38:38	antigen A	30:38	antigen A	30:38	Enterococcus faecium secreted antigen A generates muropeptides to enhance host immunity and limit bacterial pathogenesis.
30969170	2	18	theme	domain-containing	591:607	arg1	protein					609:615	nucleotide-binding oligomerization domain-containing protein 2	556:617	nucleotide-binding oligomerization domain-containing protein 2 (NOD2) in mammalian cells	556:643	Here we show E. faecium has unique peptidoglycan composition and remodeling activity through SagA, which generates smaller muropeptides that more effectively activates nucleotide-binding oligomerization domain-containing protein 2 (NOD2) in mammalian cells.
30969170	2	18	theme	domain-containing	591:607	arg1	NOD2					620:623	NOD2	620:623	NOD2	620:623	Here we show E. faecium has unique peptidoglycan composition and remodeling activity through SagA, which generates smaller muropeptides that more effectively activates nucleotide-binding oligomerization domain-containing protein 2 (NOD2) in mammalian cells.
30969170	1	19	dep	faecium	154:160	arg1	faecium					166:172	E. faecium	163:172	E. faecium	163:172	We discovered that Enterococcus faecium (E. faecium), a ubiquitous commensal bacterium, and its secreted peptidoglycan hydrolase (SagA) were sufficient to enhance intestinal barrier function and pathogen tolerance, but the precise biochemical mechanism was unknown.
30969170	1	20	theme	commensal	189:197	arg1	bacterium					199:207	a ubiquitous commensal bacterium	176:207	a ubiquitous commensal bacterium	176:207	We discovered that Enterococcus faecium (E. faecium), a ubiquitous commensal bacterium, and its secreted peptidoglycan hydrolase (SagA) were sufficient to enhance intestinal barrier function and pathogen tolerance, but the precise biochemical mechanism was unknown.
30969170	1	20	theme	commensal	189:197	arg1	sufficient					263:272	sufficient	263:272	sufficient	263:272	We discovered that Enterococcus faecium (E. faecium), a ubiquitous commensal bacterium, and its secreted peptidoglycan hydrolase (SagA) were sufficient to enhance intestinal barrier function and pathogen tolerance, but the precise biochemical mechanism was unknown.
30969170	2	21	theme	unique	416:421	arg1	composition					437:447	unique peptidoglycan composition	416:447	unique peptidoglycan composition	416:447	Here we show E. faecium has unique peptidoglycan composition and remodeling activity through SagA, which generates smaller muropeptides that more effectively activates nucleotide-binding oligomerization domain-containing protein 2 (NOD2) in mammalian cells.
30969170	2	22	theme	oligomerization	575:589	arg1	protein					609:615	nucleotide-binding oligomerization domain-containing protein 2	556:617	nucleotide-binding oligomerization domain-containing protein 2 (NOD2) in mammalian cells	556:643	Here we show E. faecium has unique peptidoglycan composition and remodeling activity through SagA, which generates smaller muropeptides that more effectively activates nucleotide-binding oligomerization domain-containing protein 2 (NOD2) in mammalian cells.
30969170	2	22	theme	oligomerization	575:589	arg1	NOD2					620:623	NOD2	620:623	NOD2	620:623	Here we show E. faecium has unique peptidoglycan composition and remodeling activity through SagA, which generates smaller muropeptides that more effectively activates nucleotide-binding oligomerization domain-containing protein 2 (NOD2) in mammalian cells.
30969170	5	23	theme	host	1089:1092	arg1	pathways					1101:1108	host immune pathways	1089:1108	host immune pathways	1089:1108	Our results demonstrate that the peptidoglycan composition and hydrolase activity of specific microbiota species can activate host immune pathways and enhance tolerance to pathogens.
30969170	1	24	theme	intestinal	285:294	arg1	function					304:311	intestinal barrier function	285:311	intestinal barrier function	285:311	We discovered that Enterococcus faecium (E. faecium), a ubiquitous commensal bacterium, and its secreted peptidoglycan hydrolase (SagA) were sufficient to enhance intestinal barrier function and pathogen tolerance, but the precise biochemical mechanism was unknown.
30969170	2	25	theme	nucleotide-binding	556:573	arg1	protein					609:615	nucleotide-binding oligomerization domain-containing protein 2	556:617	nucleotide-binding oligomerization domain-containing protein 2 (NOD2) in mammalian cells	556:643	Here we show E. faecium has unique peptidoglycan composition and remodeling activity through SagA, which generates smaller muropeptides that more effectively activates nucleotide-binding oligomerization domain-containing protein 2 (NOD2) in mammalian cells.
30969170	2	25	theme	nucleotide-binding	556:573	arg1	NOD2					620:623	NOD2	620:623	NOD2	620:623	Here we show E. faecium has unique peptidoglycan composition and remodeling activity through SagA, which generates smaller muropeptides that more effectively activates nucleotide-binding oligomerization domain-containing protein 2 (NOD2) in mammalian cells.
30969170	4	26	theme	Clostridium	919:929	arg1	pathogenesis					941:952	Clostridium difficile pathogenesis	919:952	Clostridium difficile pathogenesis in vivo	919:960	SagA secretion and NlpC/p60-endopeptidase activity was required for enhancing probiotic bacteria activity against Clostridium difficile pathogenesis in vivo.
30969170	3	27	link	crosslinked	759:769	arg1	fragments					794:802	crosslinked Lys-type peptidoglycan fragments	759:802	crosslinked Lys-type peptidoglycan fragments	759:802	Our structural and biochemical studies show that SagA is a NlpC/p60-endopeptidase that preferentially hydrolyzes crosslinked Lys-type peptidoglycan fragments.
30969170	1	28	theme	barrier	296:302	arg1	function					304:311	intestinal barrier function	285:311	intestinal barrier function	285:311	We discovered that Enterococcus faecium (E. faecium), a ubiquitous commensal bacterium, and its secreted peptidoglycan hydrolase (SagA) were sufficient to enhance intestinal barrier function and pathogen tolerance, but the precise biochemical mechanism was unknown.
30969170	3	29	theme	biochemical	665:675	arg1	studies					677:683	Our structural and biochemical studies	646:683	Our structural and biochemical studies	646:683	Our structural and biochemical studies show that SagA is a NlpC/p60-endopeptidase that preferentially hydrolyzes crosslinked Lys-type peptidoglycan fragments.
30969170	2	30	theme	peptidoglycan	423:435	arg1	composition					437:447	unique peptidoglycan composition	416:447	unique peptidoglycan composition	416:447	Here we show E. faecium has unique peptidoglycan composition and remodeling activity through SagA, which generates smaller muropeptides that more effectively activates nucleotide-binding oligomerization domain-containing protein 2 (NOD2) in mammalian cells.
30969170	2	31	theme	remodeling	453:462	arg1	activity					464:471	remodeling activity	453:471	remodeling activity	453:471	Here we show E. faecium has unique peptidoglycan composition and remodeling activity through SagA, which generates smaller muropeptides that more effectively activates nucleotide-binding oligomerization domain-containing protein 2 (NOD2) in mammalian cells.
30969170	4	32	theme	difficile	931:939	arg1	pathogenesis					941:952	Clostridium difficile pathogenesis	919:952	Clostridium difficile pathogenesis in vivo	919:960	SagA secretion and NlpC/p60-endopeptidase activity was required for enhancing probiotic bacteria activity against Clostridium difficile pathogenesis in vivo.
30969170	1	33	theme	pathogen	317:324	arg1	tolerance					326:334	pathogen tolerance	317:334	pathogen tolerance	317:334	We discovered that Enterococcus faecium (E. faecium), a ubiquitous commensal bacterium, and its secreted peptidoglycan hydrolase (SagA) were sufficient to enhance intestinal barrier function and pathogen tolerance, but the precise biochemical mechanism was unknown.
30969170	4	34	theme	probiotic	883:891	arg1	bacteria					893:900	probiotic bacteria	883:900	probiotic bacteria activity against Clostridium difficile pathogenesis in vivo	883:960	SagA secretion and NlpC/p60-endopeptidase activity was required for enhancing probiotic bacteria activity against Clostridium difficile pathogenesis in vivo.
30969170	3	35	theme	structural	650:659	arg1	studies					677:683	Our structural and biochemical studies	646:683	Our structural and biochemical studies	646:683	Our structural and biochemical studies show that SagA is a NlpC/p60-endopeptidase that preferentially hydrolyzes crosslinked Lys-type peptidoglycan fragments.
30969170	2	36	dep	show	396:399	arg1	has					412:414	has	412:414	show E. faecium has unique peptidoglycan composition and remodeling activity through SagA, which generates smaller muropeptides that more effectively activates nucleotide-binding oligomerization domain-containing protein 2 (NOD2) in mammalian cells	396:643	Here we show E. faecium has unique peptidoglycan composition and remodeling activity through SagA, which generates smaller muropeptides that more effectively activates nucleotide-binding oligomerization domain-containing protein 2 (NOD2) in mammalian cells.
30969170	5	37	theme	specific	1048:1055	arg1	species					1068:1074	specific microbiota species	1048:1074	specific microbiota species	1048:1074	Our results demonstrate that the peptidoglycan composition and hydrolase activity of specific microbiota species can activate host immune pathways and enhance tolerance to pathogens.
30969170	4	38	theme	NlpC/p60-endopeptidase	824:845	arg1	activity					847:854	NlpC/p60-endopeptidase activity	824:854	NlpC/p60-endopeptidase activity	824:854	SagA secretion and NlpC/p60-endopeptidase activity was required for enhancing probiotic bacteria activity against Clostridium difficile pathogenesis in vivo.
30969170	1	39	theme	Enterococcus	141:152	arg1	faecium					154:160	Enterococcus faecium	141:160	Enterococcus faecium (E. faecium)	141:173	We discovered that Enterococcus faecium (E. faecium), a ubiquitous commensal bacterium, and its secreted peptidoglycan hydrolase (SagA) were sufficient to enhance intestinal barrier function and pathogen tolerance, but the precise biochemical mechanism was unknown.
30969170	1	40	theme	secreted	218:225	arg1	SagA					252:255	SagA	252:255	SagA	252:255	We discovered that Enterococcus faecium (E. faecium), a ubiquitous commensal bacterium, and its secreted peptidoglycan hydrolase (SagA) were sufficient to enhance intestinal barrier function and pathogen tolerance, but the precise biochemical mechanism was unknown.
30969170	1	40	theme	secreted	218:225	arg1	hydrolase					241:249	its secreted peptidoglycan hydrolase	214:249	its secreted peptidoglycan hydrolase (SagA)	214:256	We discovered that Enterococcus faecium (E. faecium), a ubiquitous commensal bacterium, and its secreted peptidoglycan hydrolase (SagA) were sufficient to enhance intestinal barrier function and pathogen tolerance, but the precise biochemical mechanism was unknown.
30969170	2	41	theme	smaller	503:509	arg1	muropeptides					511:522	smaller muropeptides	503:522	smaller muropeptides that more effectively activates nucleotide-binding oligomerization domain-containing protein 2 (NOD2) in mammalian cells	503:643	Here we show E. faecium has unique peptidoglycan composition and remodeling activity through SagA, which generates smaller muropeptides that more effectively activates nucleotide-binding oligomerization domain-containing protein 2 (NOD2) in mammalian cells.
30969170	5	42	theme	peptidoglycan	996:1008	arg1	composition					1010:1020	peptidoglycan composition	996:1020	peptidoglycan composition	996:1020	Our results demonstrate that the peptidoglycan composition and hydrolase activity of specific microbiota species can activate host immune pathways and enhance tolerance to pathogens.
30969170	1	43	theme	peptidoglycan	227:239	arg1	SagA					252:255	SagA	252:255	SagA	252:255	We discovered that Enterococcus faecium (E. faecium), a ubiquitous commensal bacterium, and its secreted peptidoglycan hydrolase (SagA) were sufficient to enhance intestinal barrier function and pathogen tolerance, but the precise biochemical mechanism was unknown.
30969170	1	43	theme	peptidoglycan	227:239	arg1	hydrolase					241:249	its secreted peptidoglycan hydrolase	214:249	its secreted peptidoglycan hydrolase (SagA)	214:256	We discovered that Enterococcus faecium (E. faecium), a ubiquitous commensal bacterium, and its secreted peptidoglycan hydrolase (SagA) were sufficient to enhance intestinal barrier function and pathogen tolerance, but the precise biochemical mechanism was unknown.
30969170	4	44	theme	bacteria	893:900	arg1	activity					902:909	probiotic bacteria activity	883:909	probiotic bacteria activity against Clostridium difficile pathogenesis in vivo	883:960	SagA secretion and NlpC/p60-endopeptidase activity was required for enhancing probiotic bacteria activity against Clostridium difficile pathogenesis in vivo.
30816709	6	0	theme	c-Jun	707:711	arg1	kinase					724:729	c-Jun N-terminal kinase	707:729	c-Jun N-terminal kinase (JNK)	707:735	The levels of synaptic plasticity markers including postsynaptic density protein 95 (PSD-95) and synapsin I, as well as phosphorylation of c-Jun N-terminal kinase (JNK), were determined.
30816709	6	0	theme	c-Jun	707:711	arg1	JNK					732:734	JNK	732:734	JNK	732:734	The levels of synaptic plasticity markers including postsynaptic density protein 95 (PSD-95) and synapsin I, as well as phosphorylation of c-Jun N-terminal kinase (JNK), were determined.
30816709	9	1	theme	cognitive	1005:1013	arg1	deficits					1015:1022	cognitive deficits	1005:1022	cognitive deficits	1005:1022	The results indicated that FOS treatment ameliorated cognitive deficits and pathological changes in the Tg mice.
30816709	10	2	theme	phosphorylated	1164:1177	arg1	level					1179:1183	decreased phosphorylated level	1154:1183	decreased phosphorylated level of JNK	1154:1190	FOS significantly upregulated the expression levels of synapsin I and PSD-95, as well as decreased phosphorylated level of JNK.
30816709	3	3	dep	effect	337:342	arg1	the					333:335	the	333:335	the	333:335	The present study aimed to explore the effect and mechanism of FOS protection against AD via regulating gut microbiota.
30816709	6	4	theme	density	633:639	arg1	protein					641:647	postsynaptic density protein 95	620:650	postsynaptic density protein 95 (PSD-95)	620:659	The levels of synaptic plasticity markers including postsynaptic density protein 95 (PSD-95) and synapsin I, as well as phosphorylation of c-Jun N-terminal kinase (JNK), were determined.
30816709	6	4	theme	density	633:639	arg1	PSD-95					653:658	PSD-95	653:658	PSD-95	653:658	The levels of synaptic plasticity markers including postsynaptic density protein 95 (PSD-95) and synapsin I, as well as phosphorylation of c-Jun N-terminal kinase (JNK), were determined.
30816709	10	5	theme	decreased	1154:1162	arg1	level					1179:1183	decreased phosphorylated level	1154:1183	decreased phosphorylated level of JNK	1154:1190	FOS significantly upregulated the expression levels of synapsin I and PSD-95, as well as decreased phosphorylated level of JNK.
30816709	3	6	theme	protection	365:374	arg1	mechanism					348:356	mechanism	348:356	mechanism	348:356	The present study aimed to explore the effect and mechanism of FOS protection against AD via regulating gut microbiota.
30816709	3	6	theme	protection	365:374	arg1	effect					337:342	effect	337:342	effect	337:342	The present study aimed to explore the effect and mechanism of FOS protection against AD via regulating gut microbiota.
30816709	12	7	theme	GLP-1R	1349:1354	arg1	level					1340:1344	the level	1336:1344	the level of GLP-1R in the Tg mice	1336:1369	Furthermore, FOS increased the level of GLP-1 and decreased the level of GLP-1R in the Tg mice.
30816709	6	8	theme	postsynaptic	620:631	arg1	protein					641:647	postsynaptic density protein 95	620:650	postsynaptic density protein 95 (PSD-95)	620:659	The levels of synaptic plasticity markers including postsynaptic density protein 95 (PSD-95) and synapsin I, as well as phosphorylation of c-Jun N-terminal kinase (JNK), were determined.
30816709	6	8	theme	postsynaptic	620:631	arg1	PSD-95					653:658	PSD-95	653:658	PSD-95	653:658	The levels of synaptic plasticity markers including postsynaptic density protein 95 (PSD-95) and synapsin I, as well as phosphorylation of c-Jun N-terminal kinase (JNK), were determined.
30816709	0	9	theme	Gut	123:125	arg1	Microbiota					127:136	Gut Microbiota	123:136	Gut Microbiota	123:136	Fructooligosaccharides Ameliorating Cognitive Deficits and Neurodegeneration in APP/PS1 Transgenic Mice through Modulating Gut Microbiota.
30816709	9	10	theme	pathological	1028:1039	arg1	changes					1041:1047	pathological changes	1028:1047	pathological changes	1028:1047	The results indicated that FOS treatment ameliorated cognitive deficits and pathological changes in the Tg mice.
30816709	4	11	dep	transgenic	448:457	arg1	Tg					460:461	Tg	460:461	Tg	460:461	Male Apse/PSEN 1dE9 (APP/PS1) transgenic (Tg) mice were administrated with FOS for 6 weeks.
30816709	12	12	theme	Tg	1363:1364	arg1	mice					1366:1369	the Tg mice	1359:1369	the Tg mice	1359:1369	Furthermore, FOS increased the level of GLP-1 and decreased the level of GLP-1R in the Tg mice.
30816709	4	13	theme	Male	418:421	arg1	mice					464:467	Male Apse/PSEN 1dE9 (APP/PS1) transgenic (Tg) mice	418:467	Male Apse/PSEN 1dE9 (APP/PS1) transgenic (Tg) mice	418:467	Male Apse/PSEN 1dE9 (APP/PS1) transgenic (Tg) mice were administrated with FOS for 6 weeks.
30816709	12	14	from	level	1340:1344	arg1	mice					1366:1369	the Tg mice	1359:1369	the Tg mice	1359:1369	Furthermore, FOS increased the level of GLP-1 and decreased the level of GLP-1R in the Tg mice.
30816709	8	15	from	gut	892:894	arg1	levels					843:848	the levels	839:848	the levels of glucagon-like peptide-1 (GLP-1) in the gut and GLP-1 receptor (GLP-1R) in the brain	839:935	Moreover, the levels of glucagon-like peptide-1 (GLP-1) in the gut and GLP-1 receptor (GLP-1R) in the brain were measured.
30816709	3	16	theme	FOS	361:363	arg1	protection					365:374	FOS protection	361:374	FOS protection against AD	361:385	The present study aimed to explore the effect and mechanism of FOS protection against AD via regulating gut microbiota.
30816709	0	17	from	Neurodegeneration	59:75	arg1	Mice					99:102	APP/PS1 Transgenic Mice	80:102	APP/PS1 Transgenic Mice	80:102	Fructooligosaccharides Ameliorating Cognitive Deficits and Neurodegeneration in APP/PS1 Transgenic Mice through Modulating Gut Microbiota.
30816709	6	18	theme	markers	602:608	arg1	levels					572:577	The levels	568:577	The levels of synaptic plasticity markers including postsynaptic density protein 95 (PSD-95) and synapsin I	568:674	The levels of synaptic plasticity markers including postsynaptic density protein 95 (PSD-95) and synapsin I, as well as phosphorylation of c-Jun N-terminal kinase (JNK), were determined.
30816709	6	18	theme	markers	602:608	arg1	phosphorylation					688:702	phosphorylation	688:702	phosphorylation of c-Jun N-terminal kinase (JNK)	688:735	The levels of synaptic plasticity markers including postsynaptic density protein 95 (PSD-95) and synapsin I, as well as phosphorylation of c-Jun N-terminal kinase (JNK), were determined.
30816709	8	19	theme	peptide-1	867:875	arg1	levels					843:848	the levels	839:848	the levels of glucagon-like peptide-1 (GLP-1) in the gut and GLP-1 receptor (GLP-1R) in the brain	839:935	Moreover, the levels of glucagon-like peptide-1 (GLP-1) in the gut and GLP-1 receptor (GLP-1R) in the brain were measured.
30816709	1	20	theme	gut	186:188	arg1	alteration					200:209	gut microbial alteration	186:209	gut microbial alteration	186:209	Alzheimer's disease (AD) is closely related to gut microbial alteration.
30816709	0	21	theme	Ameliorating	23:34	arg1	Deficits					46:53	Ameliorating Cognitive Deficits	23:53	Ameliorating Cognitive Deficits	23:53	Fructooligosaccharides Ameliorating Cognitive Deficits and Neurodegeneration in APP/PS1 Transgenic Mice through Modulating Gut Microbiota.
30816709	11	22	theme	altered	1245:1251	arg1	composition					1263:1273	the altered microbial composition	1241:1273	the altered microbial composition	1241:1273	The sequencing results showed that FOS reversed the altered microbial composition.
30816709	8	23	theme	glucagon-like	853:865	arg1	GLP-1					878:882	GLP-1	878:882	GLP-1	878:882	Moreover, the levels of glucagon-like peptide-1 (GLP-1) in the gut and GLP-1 receptor (GLP-1R) in the brain were measured.
30816709	8	23	theme	glucagon-like	853:865	arg1	peptide-1					867:875	glucagon-like peptide-1	853:875	glucagon-like peptide-1 (GLP-1) in the gut	853:894	Moreover, the levels of glucagon-like peptide-1 (GLP-1) in the gut and GLP-1 receptor (GLP-1R) in the brain were measured.
30816709	1	24	theme	microbial	190:198	arg1	alteration					200:209	gut microbial alteration	186:209	gut microbial alteration	186:209	Alzheimer's disease (AD) is closely related to gut microbial alteration.
30816709	3	25	theme	gut	402:404	arg1	microbiota					406:415	gut microbiota	402:415	gut microbiota	402:415	The present study aimed to explore the effect and mechanism of FOS protection against AD via regulating gut microbiota.
30816709	10	26	theme	expression	1099:1108	arg1	levels					1110:1115	the expression levels	1095:1115	the expression levels of synapsin I and PSD-95	1095:1140	FOS significantly upregulated the expression levels of synapsin I and PSD-95, as well as decreased phosphorylated level of JNK.
30816709	11	27	theme	microbial	1253:1261	arg1	composition					1263:1273	the altered microbial composition	1241:1273	the altered microbial composition	1241:1273	The sequencing results showed that FOS reversed the altered microbial composition.
30816709	7	28	theme	rRNA	812:815	arg1	sequencing					817:826	rRNA sequencing	812:826	rRNA sequencing	812:826	The intestinal microbial constituent was detected by 16S rRNA sequencing.
30816709	6	29	theme	plasticity	591:600	arg1	protein					641:647	postsynaptic density protein 95	620:650	postsynaptic density protein 95 (PSD-95)	620:659	The levels of synaptic plasticity markers including postsynaptic density protein 95 (PSD-95) and synapsin I, as well as phosphorylation of c-Jun N-terminal kinase (JNK), were determined.
30816709	6	29	theme	plasticity	591:600	arg1	synapsin					665:672	synapsin I	665:674	synapsin I	665:674	The levels of synaptic plasticity markers including postsynaptic density protein 95 (PSD-95) and synapsin I, as well as phosphorylation of c-Jun N-terminal kinase (JNK), were determined.
30816709	6	29	theme	plasticity	591:600	arg1	markers					602:608	synaptic plasticity markers	582:608	synaptic plasticity markers including postsynaptic density protein 95 (PSD-95) and synapsin I	582:674	The levels of synaptic plasticity markers including postsynaptic density protein 95 (PSD-95) and synapsin I, as well as phosphorylation of c-Jun N-terminal kinase (JNK), were determined.
30816709	8	30	from	peptide-1	867:875	arg1	gut					892:894	the gut	888:894	the gut	888:894	Moreover, the levels of glucagon-like peptide-1 (GLP-1) in the gut and GLP-1 receptor (GLP-1R) in the brain were measured.
30816709	0	31	theme	Cognitive	36:44	arg1	Deficits					46:53	Ameliorating Cognitive Deficits	23:53	Ameliorating Cognitive Deficits	23:53	Fructooligosaccharides Ameliorating Cognitive Deficits and Neurodegeneration in APP/PS1 Transgenic Mice through Modulating Gut Microbiota.
30816709	7	32	theme	microbial	770:778	arg1	constituent					780:790	The intestinal microbial constituent	755:790	The intestinal microbial constituent	755:790	The intestinal microbial constituent was detected by 16S rRNA sequencing.
30816709	3	33	theme	present	302:308	arg1	study					310:314	The present study	298:314	The present study	298:314	The present study aimed to explore the effect and mechanism of FOS protection against AD via regulating gut microbiota.
30816709	9	34	theme	Tg	1056:1057	arg1	mice					1059:1062	the Tg mice	1052:1062	the Tg mice	1052:1062	The results indicated that FOS treatment ameliorated cognitive deficits and pathological changes in the Tg mice.
30816709	4	35	theme	transgenic	448:457	arg1	mice					464:467	Male Apse/PSEN 1dE9 (APP/PS1) transgenic (Tg) mice	418:467	Male Apse/PSEN 1dE9 (APP/PS1) transgenic (Tg) mice	418:467	Male Apse/PSEN 1dE9 (APP/PS1) transgenic (Tg) mice were administrated with FOS for 6 weeks.
30816709	2	36	theme	major	256:260	arg1	roles					262:266	major roles	256:266	major roles	256:266	Prebiotic fructooligosaccharides (FOS) play major roles by regulating gut microbiota.
30816709	8	37	theme	receptor	906:913	arg1	levels					843:848	the levels	839:848	the levels of glucagon-like peptide-1 (GLP-1) in the gut and GLP-1 receptor (GLP-1R) in the brain	839:935	Moreover, the levels of glucagon-like peptide-1 (GLP-1) in the gut and GLP-1 receptor (GLP-1R) in the brain were measured.
30816709	5	38	theme	amyloid	533:539	arg1	deposition					541:550	amyloid deposition	533:550	amyloid deposition	533:550	Cognitive deficits and amyloid deposition were evaluated.
30816709	6	39	theme	kinase	724:729	arg1	levels					572:577	The levels	568:577	The levels of synaptic plasticity markers including postsynaptic density protein 95 (PSD-95) and synapsin I	568:674	The levels of synaptic plasticity markers including postsynaptic density protein 95 (PSD-95) and synapsin I, as well as phosphorylation of c-Jun N-terminal kinase (JNK), were determined.
30816709	6	39	theme	kinase	724:729	arg1	phosphorylation					688:702	phosphorylation	688:702	phosphorylation of c-Jun N-terminal kinase (JNK)	688:735	The levels of synaptic plasticity markers including postsynaptic density protein 95 (PSD-95) and synapsin I, as well as phosphorylation of c-Jun N-terminal kinase (JNK), were determined.
30816709	0	40	theme	APP/PS1	80:86	arg1	Mice					99:102	APP/PS1 Transgenic Mice	80:102	APP/PS1 Transgenic Mice	80:102	Fructooligosaccharides Ameliorating Cognitive Deficits and Neurodegeneration in APP/PS1 Transgenic Mice through Modulating Gut Microbiota.
30816709	7	41	theme	intestinal	759:768	arg1	constituent					780:790	The intestinal microbial constituent	755:790	The intestinal microbial constituent	755:790	The intestinal microbial constituent was detected by 16S rRNA sequencing.
30816709	12	42	theme	GLP-1	1316:1320	arg1	level					1307:1311	the level	1303:1311	the level of GLP-1	1303:1320	Furthermore, FOS increased the level of GLP-1 and decreased the level of GLP-1R in the Tg mice.
30816709	13	43	theme	beneficial	1414:1423	arg1	effects					1425:1431	beneficial effects	1414:1431	beneficial effects	1414:1431	These findings indicated that FOS exerted beneficial effects against AD via regulating the gut microbiota-GLP-1/GLP-1R pathway.
30816709	8	44	from	levels	843:848	arg1	brain					931:935	the brain	927:935	the brain	927:935	Moreover, the levels of glucagon-like peptide-1 (GLP-1) in the gut and GLP-1 receptor (GLP-1R) in the brain were measured.
30816709	8	44	from	levels	843:848	arg1	gut					892:894	the gut	888:894	the gut	888:894	Moreover, the levels of glucagon-like peptide-1 (GLP-1) in the gut and GLP-1 receptor (GLP-1R) in the brain were measured.
30816709	8	45	theme	GLP-1	900:904	arg1	GLP-1R					916:921	GLP-1R	916:921	GLP-1R	916:921	Moreover, the levels of glucagon-like peptide-1 (GLP-1) in the gut and GLP-1 receptor (GLP-1R) in the brain were measured.
30816709	8	45	theme	GLP-1	900:904	arg1	receptor					906:913	GLP-1 receptor	900:913	GLP-1 receptor (GLP-1R)	900:922	Moreover, the levels of glucagon-like peptide-1 (GLP-1) in the gut and GLP-1 receptor (GLP-1R) in the brain were measured.
30816709	13	46	theme	gut	1463:1465	arg1	pathway					1491:1497	the gut microbiota-GLP-1/GLP-1R pathway	1459:1497	the gut microbiota-GLP-1/GLP-1R pathway	1459:1497	These findings indicated that FOS exerted beneficial effects against AD via regulating the gut microbiota-GLP-1/GLP-1R pathway.
30816709	2	47	theme	gut	282:284	arg1	microbiota					286:295	gut microbiota	282:295	gut microbiota	282:295	Prebiotic fructooligosaccharides (FOS) play major roles by regulating gut microbiota.
30816709	10	48	theme	JNK	1188:1190	arg1	level					1179:1183	decreased phosphorylated level	1154:1183	decreased phosphorylated level of JNK	1154:1190	FOS significantly upregulated the expression levels of synapsin I and PSD-95, as well as decreased phosphorylated level of JNK.
30816709	10	48	theme	JNK	1188:1190	arg1	levels					1110:1115	the expression levels	1095:1115	the expression levels of synapsin I and PSD-95	1095:1140	FOS significantly upregulated the expression levels of synapsin I and PSD-95, as well as decreased phosphorylated level of JNK.
30816709	11	49	theme	sequencing	1197:1206	arg1	results					1208:1214	The sequencing results	1193:1214	The sequencing results	1193:1214	The sequencing results showed that FOS reversed the altered microbial composition.
30816709	2	50	theme	Prebiotic	212:220	arg1	FOS					246:248	FOS	246:248	FOS	246:248	Prebiotic fructooligosaccharides (FOS) play major roles by regulating gut microbiota.
30816709	2	50	theme	Prebiotic	212:220	arg1	fructooligosaccharides					222:243	Prebiotic fructooligosaccharides	212:243	Prebiotic fructooligosaccharides (FOS)	212:249	Prebiotic fructooligosaccharides (FOS) play major roles by regulating gut microbiota.
30816709	13	51	theme	microbiota-GLP-1/GLP-1R	1467:1489	arg1	pathway					1491:1497	the gut microbiota-GLP-1/GLP-1R pathway	1459:1497	the gut microbiota-GLP-1/GLP-1R pathway	1459:1497	These findings indicated that FOS exerted beneficial effects against AD via regulating the gut microbiota-GLP-1/GLP-1R pathway.
30816709	9	52	theme	FOS	979:981	arg1	treatment					983:991	FOS treatment	979:991	FOS treatment	979:991	The results indicated that FOS treatment ameliorated cognitive deficits and pathological changes in the Tg mice.
30816709	8	53	from	receptor	906:913	arg1	gut					892:894	the gut	888:894	the gut	888:894	Moreover, the levels of glucagon-like peptide-1 (GLP-1) in the gut and GLP-1 receptor (GLP-1R) in the brain were measured.
30816709	5	54	theme	Cognitive	510:518	arg1	deficits					520:527	Cognitive deficits	510:527	Cognitive deficits	510:527	Cognitive deficits and amyloid deposition were evaluated.
30816709	10	55	theme	PSD-95	1135:1140	arg1	level					1179:1183	decreased phosphorylated level	1154:1183	decreased phosphorylated level of JNK	1154:1190	FOS significantly upregulated the expression levels of synapsin I and PSD-95, as well as decreased phosphorylated level of JNK.
30816709	10	55	theme	PSD-95	1135:1140	arg1	levels					1110:1115	the expression levels	1095:1115	the expression levels of synapsin I and PSD-95	1095:1140	FOS significantly upregulated the expression levels of synapsin I and PSD-95, as well as decreased phosphorylated level of JNK.
30816709	0	56	theme	Transgenic	88:97	arg1	Mice					99:102	APP/PS1 Transgenic Mice	80:102	APP/PS1 Transgenic Mice	80:102	Fructooligosaccharides Ameliorating Cognitive Deficits and Neurodegeneration in APP/PS1 Transgenic Mice through Modulating Gut Microbiota.
30816709	0	57	from	Deficits	46:53	arg1	Mice					99:102	APP/PS1 Transgenic Mice	80:102	APP/PS1 Transgenic Mice	80:102	Fructooligosaccharides Ameliorating Cognitive Deficits and Neurodegeneration in APP/PS1 Transgenic Mice through Modulating Gut Microbiota.
30816709	6	58	theme	synaptic	582:589	arg1	protein					641:647	postsynaptic density protein 95	620:650	postsynaptic density protein 95 (PSD-95)	620:659	The levels of synaptic plasticity markers including postsynaptic density protein 95 (PSD-95) and synapsin I, as well as phosphorylation of c-Jun N-terminal kinase (JNK), were determined.
30816709	6	58	theme	synaptic	582:589	arg1	synapsin					665:672	synapsin I	665:674	synapsin I	665:674	The levels of synaptic plasticity markers including postsynaptic density protein 95 (PSD-95) and synapsin I, as well as phosphorylation of c-Jun N-terminal kinase (JNK), were determined.
30816709	6	58	theme	synaptic	582:589	arg1	markers					602:608	synaptic plasticity markers	582:608	synaptic plasticity markers including postsynaptic density protein 95 (PSD-95) and synapsin I	582:674	The levels of synaptic plasticity markers including postsynaptic density protein 95 (PSD-95) and synapsin I, as well as phosphorylation of c-Jun N-terminal kinase (JNK), were determined.
30816709	6	59	theme	N-terminal	713:722	arg1	kinase					724:729	c-Jun N-terminal kinase	707:729	c-Jun N-terminal kinase (JNK)	707:735	The levels of synaptic plasticity markers including postsynaptic density protein 95 (PSD-95) and synapsin I, as well as phosphorylation of c-Jun N-terminal kinase (JNK), were determined.
30816709	6	59	theme	N-terminal	713:722	arg1	JNK					732:734	JNK	732:734	JNK	732:734	The levels of synaptic plasticity markers including postsynaptic density protein 95 (PSD-95) and synapsin I, as well as phosphorylation of c-Jun N-terminal kinase (JNK), were determined.
30816709	10	60	theme	synapsin	1120:1127	arg1	level					1179:1183	decreased phosphorylated level	1154:1183	decreased phosphorylated level of JNK	1154:1190	FOS significantly upregulated the expression levels of synapsin I and PSD-95, as well as decreased phosphorylated level of JNK.
30816709	10	60	theme	synapsin	1120:1127	arg1	levels					1110:1115	the expression levels	1095:1115	the expression levels of synapsin I and PSD-95	1095:1140	FOS significantly upregulated the expression levels of synapsin I and PSD-95, as well as decreased phosphorylated level of JNK.
31113503	2	0	theme	green	551:555	arg1	fodder					557:562	a mixed-species green fodder	535:562	lactating dairy goats a mixed-species green fodder (MF, consisting of perennial ryegrass, timothy (Phleum pratense L.), prairie grass (Bromus willdenowii Kunth), white clover, red clover (Trifolium pratense L.), lucerne (Medicago sativa L.), chicory (Cichorium intybus L.) and plantain (Plantago lanceolata L.) improves dietary intake, milk yield and composition compared with a standard ryegrass and white clover green fodder (SF)	513:943	The aim of this experiment was to determine whether feeding lactating dairy goats a mixed-species green fodder (MF, consisting of perennial ryegrass, timothy (Phleum pratense L.), prairie grass (Bromus willdenowii Kunth), white clover, red clover (Trifolium pratense L.), lucerne (Medicago sativa L.), chicory (Cichorium intybus L.) and plantain (Plantago lanceolata L.) improves dietary intake, milk yield and composition compared with a standard ryegrass and white clover green fodder (SF).
31113503	2	1	dep	Plantago	800:807	arg1	L.					820:821	Plantago lanceolata L.	800:821	Plantago lanceolata L.	800:821	The aim of this experiment was to determine whether feeding lactating dairy goats a mixed-species green fodder (MF, consisting of perennial ryegrass, timothy (Phleum pratense L.), prairie grass (Bromus willdenowii Kunth), white clover, red clover (Trifolium pratense L.), lucerne (Medicago sativa L.), chicory (Cichorium intybus L.) and plantain (Plantago lanceolata L.) improves dietary intake, milk yield and composition compared with a standard ryegrass and white clover green fodder (SF).
31113503	9	2	theme	linoleic	1895:1902	arg1	C18:2					1910:1914	C18:2	1910:1914	C18:2	1910:1914	An effect of green fodder type on milk fat profile was demonstrated, with proportions of pentadecylic acid (C15:0), cis-vaccenic acid (C18:1 c11), linoleic acid (C18:2 n6) and α-linolenic acid (C18:3 n3) being increased in response to MF consumption.
31113503	9	2	theme	linoleic	1895:1902	arg1	acid					1904:1907	linoleic acid	1895:1907	linoleic acid (C18:2 n6)	1895:1918	An effect of green fodder type on milk fat profile was demonstrated, with proportions of pentadecylic acid (C15:0), cis-vaccenic acid (C18:1 c11), linoleic acid (C18:2 n6) and α-linolenic acid (C18:3 n3) being increased in response to MF consumption.
31113503	7	3	from	effect	1583:1588	arg1	concentration					1606:1618	milk lactose concentration	1593:1618	milk lactose concentration	1593:1618	In addition, the milk protein and fat concentration of goats fed MF were 4% greater than for those fed SF, whereas there was no effect on milk lactose concentration.
31113503	5	4	theme	group	1296:1300	arg1	B					1302:1302	group B	1296:1302	group B	1296:1302	For P1, group A was fed MF and group B was fed SF, and then the group diets were switched for P2.
31113503	9	5	from	effect	1751:1756	arg1	profile					1791:1797	milk fat profile	1782:1797	milk fat profile	1782:1797	An effect of green fodder type on milk fat profile was demonstrated, with proportions of pentadecylic acid (C15:0), cis-vaccenic acid (C18:1 c11), linoleic acid (C18:2 n6) and α-linolenic acid (C18:3 n3) being increased in response to MF consumption.
31113503	1	6	theme	dairy	151:155	arg1	farmers					162:168	New Zealand dairy goat farmers	139:168	New Zealand dairy goat farmers	139:168	The majority of New Zealand dairy goat farmers utilise cultivated green-fed fodder dominated by perennial ryegrass (Lolium perenne L.) and white clover (Trifolium repens L.), but evidence from other ruminant species suggests that milk production may be improved when using a more diverse array of species within the green fodder.
31113503	7	7	theme	lactose	1598:1604	arg1	concentration					1606:1618	milk lactose concentration	1593:1618	milk lactose concentration	1593:1618	In addition, the milk protein and fat concentration of goats fed MF were 4% greater than for those fed SF, whereas there was no effect on milk lactose concentration.
31113503	12	8	theme	fatty	2224:2228	arg1	profile					2235:2241	the fatty acid profile	2220:2241	the fatty acid profile of goat's milk	2220:2256	The green fodder type affected the fatty acid profile of goat's milk, with MF increasing the levels of beneficial polyunsaturated omega fatty acids (linoleic and α-linolenic acids).
31113503	1	9	theme	species	420:426	arg1	array					411:415	a more diverse array	396:415	a more diverse array of species within the green fodder	396:450	The majority of New Zealand dairy goat farmers utilise cultivated green-fed fodder dominated by perennial ryegrass (Lolium perenne L.) and white clover (Trifolium repens L.), but evidence from other ruminant species suggests that milk production may be improved when using a more diverse array of species within the green fodder.
31113503	1	10	theme	farmers	162:168	arg1	majority					127:134	The majority	123:134	The majority of New Zealand dairy goat farmers	123:168	The majority of New Zealand dairy goat farmers utilise cultivated green-fed fodder dominated by perennial ryegrass (Lolium perenne L.) and white clover (Trifolium repens L.), but evidence from other ruminant species suggests that milk production may be improved when using a more diverse array of species within the green fodder.
31113503	4	11	theme	fodder	1179:1184	arg1	types					1186:1190	both green fodder types	1168:1190	both green fodder types	1168:1190	The trial was split into three periods - firstly a uniformity period of 6 days, in which all goats were fed a combination of both green fodder types, followed by two treatment periods (P1 and P2) of 12 days, respectively.
31113503	0	12	theme	housed	103:108	arg1	goats					116:120	housed dairy goats	103:120	housed dairy goats	103:120	The effect of cultivated mixed-species green fodder on intake, milk production and milk composition of housed dairy goats.
31113503	9	13	theme	acid	1936:1939	arg1	proportions					1822:1832	proportions	1822:1832	proportions of pentadecylic acid (C15:0), cis-vaccenic acid (C18:1 c11), linoleic acid (C18:2 n6) and α-linolenic acid (C18:3 n3)	1822:1950	An effect of green fodder type on milk fat profile was demonstrated, with proportions of pentadecylic acid (C15:0), cis-vaccenic acid (C18:1 c11), linoleic acid (C18:2 n6) and α-linolenic acid (C18:3 n3) being increased in response to MF consumption.
31113503	1	14	theme	cultivated	178:187	arg1	fodder					199:204	cultivated green-fed fodder	178:204	cultivated green-fed fodder dominated by perennial ryegrass (Lolium perenne L.) and white clover (Trifolium repens L.)	178:295	The majority of New Zealand dairy goat farmers utilise cultivated green-fed fodder dominated by perennial ryegrass (Lolium perenne L.) and white clover (Trifolium repens L.), but evidence from other ruminant species suggests that milk production may be improved when using a more diverse array of species within the green fodder.
31113503	12	15	theme	fodder	2199:2204	arg1	type					2206:2209	The green fodder type	2189:2209	The green fodder type	2189:2209	The green fodder type affected the fatty acid profile of goat's milk, with MF increasing the levels of beneficial polyunsaturated omega fatty acids (linoleic and α-linolenic acids).
31113503	2	16	theme	white	675:679	arg1	clover					681:686	white clover	675:686	white clover	675:686	The aim of this experiment was to determine whether feeding lactating dairy goats a mixed-species green fodder (MF, consisting of perennial ryegrass, timothy (Phleum pratense L.), prairie grass (Bromus willdenowii Kunth), white clover, red clover (Trifolium pratense L.), lucerne (Medicago sativa L.), chicory (Cichorium intybus L.) and plantain (Plantago lanceolata L.) improves dietary intake, milk yield and composition compared with a standard ryegrass and white clover green fodder (SF).
31113503	0	17	theme	goats	116:120	arg1	intake					55:60	intake	55:60	intake	55:60	The effect of cultivated mixed-species green fodder on intake, milk production and milk composition of housed dairy goats.
31113503	0	17	theme	goats	116:120	arg1	production					68:77	milk production	63:77	milk production	63:77	The effect of cultivated mixed-species green fodder on intake, milk production and milk composition of housed dairy goats.
31113503	0	17	theme	goats	116:120	arg1	composition					88:98	milk composition	83:98	milk composition	83:98	The effect of cultivated mixed-species green fodder on intake, milk production and milk composition of housed dairy goats.
31113503	1	18	theme	milk	353:356	arg1	production					358:367	milk production	353:367	milk production	353:367	The majority of New Zealand dairy goat farmers utilise cultivated green-fed fodder dominated by perennial ryegrass (Lolium perenne L.) and white clover (Trifolium repens L.), but evidence from other ruminant species suggests that milk production may be improved when using a more diverse array of species within the green fodder.
31113503	9	19	theme	C18:3	1942:1946	arg1	n3					1948:1949	C18:3 n3	1942:1949	C18:3 n3	1942:1949	An effect of green fodder type on milk fat profile was demonstrated, with proportions of pentadecylic acid (C15:0), cis-vaccenic acid (C18:1 c11), linoleic acid (C18:2 n6) and α-linolenic acid (C18:3 n3) being increased in response to MF consumption.
31113503	9	19	theme	C18:3	1942:1946	arg1	acid					1936:1939	α-linolenic acid	1924:1939	α-linolenic acid (C18:3 n3)	1924:1950	An effect of green fodder type on milk fat profile was demonstrated, with proportions of pentadecylic acid (C15:0), cis-vaccenic acid (C18:1 c11), linoleic acid (C18:2 n6) and α-linolenic acid (C18:3 n3) being increased in response to MF consumption.
31113503	2	20	theme	dairy	523:527	arg1	goats					529:533	lactating dairy goats a mixed-species green fodder (MF, consisting of perennial ryegrass, timothy (Phleum pratense L.), prairie grass (Bromus willdenowii Kunth), white clover, red clover (Trifolium pratense L.), lucerne (Medicago sativa L.), chicory (Cichorium intybus L.) and plantain (Plantago lanceolata L.) improves dietary intake, milk yield and composition compared with a standard ryegrass and white clover green fodder (SF)	513:943	lactating dairy goats a mixed-species green fodder (MF, consisting of perennial ryegrass, timothy (Phleum pratense L.), prairie grass (Bromus willdenowii Kunth), white clover, red clover (Trifolium pratense L.), lucerne (Medicago sativa L.), chicory (Cichorium intybus L.) and plantain (Plantago lanceolata L.) improves dietary intake, milk yield and composition compared with a standard ryegrass and white clover green fodder (SF)	513:943	The aim of this experiment was to determine whether feeding lactating dairy goats a mixed-species green fodder (MF, consisting of perennial ryegrass, timothy (Phleum pratense L.), prairie grass (Bromus willdenowii Kunth), white clover, red clover (Trifolium pratense L.), lucerne (Medicago sativa L.), chicory (Cichorium intybus L.) and plantain (Plantago lanceolata L.) improves dietary intake, milk yield and composition compared with a standard ryegrass and white clover green fodder (SF).
31113503	9	21	theme	fodder	1767:1772	arg1	type					1774:1777	green fodder type	1761:1777	green fodder type	1761:1777	An effect of green fodder type on milk fat profile was demonstrated, with proportions of pentadecylic acid (C15:0), cis-vaccenic acid (C18:1 c11), linoleic acid (C18:2 n6) and α-linolenic acid (C18:3 n3) being increased in response to MF consumption.
31113503	3	22	dep	groups	1025:1030	arg1	groups					1025:1030	two groups	1021:1030	two groups (A and B)	1021:1040	Thirty-six mid-lactation goats were housed indoors in pairs and split into two groups (A and B).
31113503	3	22	dep	groups	1025:1030	arg1	B					1039:1039	B	1039:1039	B	1039:1039	Thirty-six mid-lactation goats were housed indoors in pairs and split into two groups (A and B).
31113503	3	22	dep	groups	1025:1030	arg1	A					1033:1033	A	1033:1033	A	1033:1033	Thirty-six mid-lactation goats were housed indoors in pairs and split into two groups (A and B).
31113503	2	23	theme	standard	892:899	arg1	ryegrass					901:908	a standard ryegrass	890:908	a standard ryegrass	890:908	The aim of this experiment was to determine whether feeding lactating dairy goats a mixed-species green fodder (MF, consisting of perennial ryegrass, timothy (Phleum pratense L.), prairie grass (Bromus willdenowii Kunth), white clover, red clover (Trifolium pratense L.), lucerne (Medicago sativa L.), chicory (Cichorium intybus L.) and plantain (Plantago lanceolata L.) improves dietary intake, milk yield and composition compared with a standard ryegrass and white clover green fodder (SF).
31113503	2	24	theme	pratense	619:626	arg1	timothy					603:609	timothy	603:609	timothy (Phleum pratense L.)	603:630	The aim of this experiment was to determine whether feeding lactating dairy goats a mixed-species green fodder (MF, consisting of perennial ryegrass, timothy (Phleum pratense L.), prairie grass (Bromus willdenowii Kunth), white clover, red clover (Trifolium pratense L.), lucerne (Medicago sativa L.), chicory (Cichorium intybus L.) and plantain (Plantago lanceolata L.) improves dietary intake, milk yield and composition compared with a standard ryegrass and white clover green fodder (SF).
31113503	2	24	theme	pratense	619:626	arg1	L.					628:629	Phleum pratense L.	612:629	Phleum pratense L.	612:629	The aim of this experiment was to determine whether feeding lactating dairy goats a mixed-species green fodder (MF, consisting of perennial ryegrass, timothy (Phleum pratense L.), prairie grass (Bromus willdenowii Kunth), white clover, red clover (Trifolium pratense L.), lucerne (Medicago sativa L.), chicory (Cichorium intybus L.) and plantain (Plantago lanceolata L.) improves dietary intake, milk yield and composition compared with a standard ryegrass and white clover green fodder (SF).
31113503	4	25	theme	treatment	1209:1217	arg1	P2					1235:1236	P2	1235:1236	P2	1235:1236	The trial was split into three periods - firstly a uniformity period of 6 days, in which all goats were fed a combination of both green fodder types, followed by two treatment periods (P1 and P2) of 12 days, respectively.
31113503	4	25	theme	treatment	1209:1217	arg1	P1					1228:1229	P1	1228:1229	P1	1228:1229	The trial was split into three periods - firstly a uniformity period of 6 days, in which all goats were fed a combination of both green fodder types, followed by two treatment periods (P1 and P2) of 12 days, respectively.
31113503	4	25	theme	treatment	1209:1217	arg1	periods					1219:1225	two treatment periods	1205:1225	two treatment periods (P1 and P2)	1205:1237	The trial was split into three periods - firstly a uniformity period of 6 days, in which all goats were fed a combination of both green fodder types, followed by two treatment periods (P1 and P2) of 12 days, respectively.
31113503	4	26	dep	periods	1074:1080	arg1	period					1105:1110	firstly a uniformity period	1084:1110	firstly a uniformity period of 6 days	1084:1120	The trial was split into three periods - firstly a uniformity period of 6 days, in which all goats were fed a combination of both green fodder types, followed by two treatment periods (P1 and P2) of 12 days, respectively.
31113503	12	27	theme	acids	2331:2335	arg1	levels					2282:2287	the levels	2278:2287	the levels of beneficial polyunsaturated omega fatty acids (linoleic and α-linolenic acids)	2278:2368	The green fodder type affected the fatty acid profile of goat's milk, with MF increasing the levels of beneficial polyunsaturated omega fatty acids (linoleic and α-linolenic acids).
31113503	9	28	theme	fat	1787:1789	arg1	profile					1791:1797	milk fat profile	1782:1797	milk fat profile	1782:1797	An effect of green fodder type on milk fat profile was demonstrated, with proportions of pentadecylic acid (C15:0), cis-vaccenic acid (C18:1 c11), linoleic acid (C18:2 n6) and α-linolenic acid (C18:3 n3) being increased in response to MF consumption.
31113503	12	29	theme	linoleic	2338:2345	arg1	acids					2363:2367	linoleic and α-linolenic acids	2338:2367	linoleic and α-linolenic acids	2338:2367	The green fodder type affected the fatty acid profile of goat's milk, with MF increasing the levels of beneficial polyunsaturated omega fatty acids (linoleic and α-linolenic acids).
31113503	12	29	theme	linoleic	2338:2345	arg1	acids					2331:2335	beneficial polyunsaturated omega fatty acids	2292:2335	beneficial polyunsaturated omega fatty acids (linoleic and α-linolenic acids)	2292:2368	The green fodder type affected the fatty acid profile of goat's milk, with MF increasing the levels of beneficial polyunsaturated omega fatty acids (linoleic and α-linolenic acids).
31113503	2	30	theme	prairie	633:639	arg1	grass					641:645	prairie grass	633:645	prairie grass (Bromus willdenowii Kunth)	633:672	The aim of this experiment was to determine whether feeding lactating dairy goats a mixed-species green fodder (MF, consisting of perennial ryegrass, timothy (Phleum pratense L.), prairie grass (Bromus willdenowii Kunth), white clover, red clover (Trifolium pratense L.), lucerne (Medicago sativa L.), chicory (Cichorium intybus L.) and plantain (Plantago lanceolata L.) improves dietary intake, milk yield and composition compared with a standard ryegrass and white clover green fodder (SF).
31113503	2	30	theme	prairie	633:639	arg1	Kunth					667:671	Kunth	667:671	Kunth	667:671	The aim of this experiment was to determine whether feeding lactating dairy goats a mixed-species green fodder (MF, consisting of perennial ryegrass, timothy (Phleum pratense L.), prairie grass (Bromus willdenowii Kunth), white clover, red clover (Trifolium pratense L.), lucerne (Medicago sativa L.), chicory (Cichorium intybus L.) and plantain (Plantago lanceolata L.) improves dietary intake, milk yield and composition compared with a standard ryegrass and white clover green fodder (SF).
31113503	0	31	theme	milk	63:66	arg1	production					68:77	milk production	63:77	milk production	63:77	The effect of cultivated mixed-species green fodder on intake, milk production and milk composition of housed dairy goats.
31113503	9	32	theme	MF	1983:1984	arg1	consumption					1986:1996	MF consumption	1983:1996	MF consumption	1983:1996	An effect of green fodder type on milk fat profile was demonstrated, with proportions of pentadecylic acid (C15:0), cis-vaccenic acid (C18:1 c11), linoleic acid (C18:2 n6) and α-linolenic acid (C18:3 n3) being increased in response to MF consumption.
31113503	1	33	theme	New	139:141	arg1	farmers					162:168	New Zealand dairy goat farmers	139:168	New Zealand dairy goat farmers	139:168	The majority of New Zealand dairy goat farmers utilise cultivated green-fed fodder dominated by perennial ryegrass (Lolium perenne L.) and white clover (Trifolium repens L.), but evidence from other ruminant species suggests that milk production may be improved when using a more diverse array of species within the green fodder.
31113503	12	34	theme	beneficial	2292:2301	arg1	acids					2363:2367	linoleic and α-linolenic acids	2338:2367	linoleic and α-linolenic acids	2338:2367	The green fodder type affected the fatty acid profile of goat's milk, with MF increasing the levels of beneficial polyunsaturated omega fatty acids (linoleic and α-linolenic acids).
31113503	12	34	theme	beneficial	2292:2301	arg1	acids					2331:2335	beneficial polyunsaturated omega fatty acids	2292:2335	beneficial polyunsaturated omega fatty acids (linoleic and α-linolenic acids)	2292:2368	The green fodder type affected the fatty acid profile of goat's milk, with MF increasing the levels of beneficial polyunsaturated omega fatty acids (linoleic and α-linolenic acids).
31113503	2	35	theme	perennial	583:591	arg1	ryegrass					593:600	perennial ryegrass	583:600	perennial ryegrass	583:600	The aim of this experiment was to determine whether feeding lactating dairy goats a mixed-species green fodder (MF, consisting of perennial ryegrass, timothy (Phleum pratense L.), prairie grass (Bromus willdenowii Kunth), white clover, red clover (Trifolium pratense L.), lucerne (Medicago sativa L.), chicory (Cichorium intybus L.) and plantain (Plantago lanceolata L.) improves dietary intake, milk yield and composition compared with a standard ryegrass and white clover green fodder (SF).
31113503	12	36	theme	omega	2319:2323	arg1	acids					2363:2367	linoleic and α-linolenic acids	2338:2367	linoleic and α-linolenic acids	2338:2367	The green fodder type affected the fatty acid profile of goat's milk, with MF increasing the levels of beneficial polyunsaturated omega fatty acids (linoleic and α-linolenic acids).
31113503	12	36	theme	omega	2319:2323	arg1	acids					2331:2335	beneficial polyunsaturated omega fatty acids	2292:2335	beneficial polyunsaturated omega fatty acids (linoleic and α-linolenic acids)	2292:2368	The green fodder type affected the fatty acid profile of goat's milk, with MF increasing the levels of beneficial polyunsaturated omega fatty acids (linoleic and α-linolenic acids).
31113503	6	37	theme	dry	1392:1394	arg1	intake					1403:1408	13% greater dry matter intake	1380:1408	13% greater dry matter intake	1380:1408	Goats fed MF had 13% greater dry matter intake and 7% greater milk yield than goats fed SF.
31113503	1	38	theme	ruminant	322:329	arg1	species					331:337	other ruminant species	316:337	other ruminant species	316:337	The majority of New Zealand dairy goat farmers utilise cultivated green-fed fodder dominated by perennial ryegrass (Lolium perenne L.) and white clover (Trifolium repens L.), but evidence from other ruminant species suggests that milk production may be improved when using a more diverse array of species within the green fodder.
31113503	2	39	theme	dietary	833:839	arg1	intake					841:846	dietary intake	833:846	dietary intake	833:846	The aim of this experiment was to determine whether feeding lactating dairy goats a mixed-species green fodder (MF, consisting of perennial ryegrass, timothy (Phleum pratense L.), prairie grass (Bromus willdenowii Kunth), white clover, red clover (Trifolium pratense L.), lucerne (Medicago sativa L.), chicory (Cichorium intybus L.) and plantain (Plantago lanceolata L.) improves dietary intake, milk yield and composition compared with a standard ryegrass and white clover green fodder (SF).
31113503	9	40	theme	pentadecylic	1837:1848	arg1	C15:0					1856:1860	C15:0	1856:1860	C15:0	1856:1860	An effect of green fodder type on milk fat profile was demonstrated, with proportions of pentadecylic acid (C15:0), cis-vaccenic acid (C18:1 c11), linoleic acid (C18:2 n6) and α-linolenic acid (C18:3 n3) being increased in response to MF consumption.
31113503	9	40	theme	pentadecylic	1837:1848	arg1	acid					1850:1853	pentadecylic acid	1837:1853	pentadecylic acid (C15:0)	1837:1861	An effect of green fodder type on milk fat profile was demonstrated, with proportions of pentadecylic acid (C15:0), cis-vaccenic acid (C18:1 c11), linoleic acid (C18:2 n6) and α-linolenic acid (C18:3 n3) being increased in response to MF consumption.
31113503	1	41	from	species	331:337	arg1	evidence					302:309	evidence	302:309	evidence from other ruminant species	302:337	The majority of New Zealand dairy goat farmers utilise cultivated green-fed fodder dominated by perennial ryegrass (Lolium perenne L.) and white clover (Trifolium repens L.), but evidence from other ruminant species suggests that milk production may be improved when using a more diverse array of species within the green fodder.
31113503	9	42	theme	acid	1850:1853	arg1	proportions					1822:1832	proportions	1822:1832	proportions of pentadecylic acid (C15:0), cis-vaccenic acid (C18:1 c11), linoleic acid (C18:2 n6) and α-linolenic acid (C18:3 n3)	1822:1950	An effect of green fodder type on milk fat profile was demonstrated, with proportions of pentadecylic acid (C15:0), cis-vaccenic acid (C18:1 c11), linoleic acid (C18:2 n6) and α-linolenic acid (C18:3 n3) being increased in response to MF consumption.
31113503	8	43	theme	protein	1668:1674	arg1	levels					1658:1663	the levels	1654:1663	the levels of protein, glucose, urea or non-esterified fatty acids	1654:1719	There was no treatment effect on the levels of protein, glucose, urea or non-esterified fatty acids in the blood of the goats.
31113503	1	44	theme	white	262:266	arg1	clover					268:273	white clover	262:273	white clover (Trifolium repens L.)	262:295	The majority of New Zealand dairy goat farmers utilise cultivated green-fed fodder dominated by perennial ryegrass (Lolium perenne L.) and white clover (Trifolium repens L.), but evidence from other ruminant species suggests that milk production may be improved when using a more diverse array of species within the green fodder.
31113503	1	44	theme	white	262:266	arg1	repens					286:291	Trifolium repens L.	276:294	Trifolium repens L.	276:294	The majority of New Zealand dairy goat farmers utilise cultivated green-fed fodder dominated by perennial ryegrass (Lolium perenne L.) and white clover (Trifolium repens L.), but evidence from other ruminant species suggests that milk production may be improved when using a more diverse array of species within the green fodder.
31113503	8	45	theme	glucose	1677:1683	arg1	levels					1658:1663	the levels	1654:1663	the levels of protein, glucose, urea or non-esterified fatty acids	1654:1719	There was no treatment effect on the levels of protein, glucose, urea or non-esterified fatty acids in the blood of the goats.
31113503	12	46	theme	milk	2253:2256	arg1	profile					2235:2241	the fatty acid profile	2220:2241	the fatty acid profile of goat's milk	2220:2256	The green fodder type affected the fatty acid profile of goat's milk, with MF increasing the levels of beneficial polyunsaturated omega fatty acids (linoleic and α-linolenic acids).
31113503	11	47	theme	green	2121:2125	arg1	intake					2134:2139	green fodder intake	2121:2139	green fodder intake	2121:2139	In summary, this study demonstrated that goats fed MF increased green fodder intake and milk production compared with goats fed SF.
31113503	2	48	theme	green	927:931	arg1	SF					941:942	SF	941:942	SF	941:942	The aim of this experiment was to determine whether feeding lactating dairy goats a mixed-species green fodder (MF, consisting of perennial ryegrass, timothy (Phleum pratense L.), prairie grass (Bromus willdenowii Kunth), white clover, red clover (Trifolium pratense L.), lucerne (Medicago sativa L.), chicory (Cichorium intybus L.) and plantain (Plantago lanceolata L.) improves dietary intake, milk yield and composition compared with a standard ryegrass and white clover green fodder (SF).
31113503	2	48	theme	green	927:931	arg1	fodder					933:938	white clover green fodder	914:938	white clover green fodder (SF)	914:943	The aim of this experiment was to determine whether feeding lactating dairy goats a mixed-species green fodder (MF, consisting of perennial ryegrass, timothy (Phleum pratense L.), prairie grass (Bromus willdenowii Kunth), white clover, red clover (Trifolium pratense L.), lucerne (Medicago sativa L.), chicory (Cichorium intybus L.) and plantain (Plantago lanceolata L.) improves dietary intake, milk yield and composition compared with a standard ryegrass and white clover green fodder (SF).
31113503	8	49	theme	treatment	1634:1642	arg1	effect					1644:1649	no treatment effect	1631:1649	no treatment effect on the levels of protein, glucose, urea or non-esterified fatty acids	1631:1719	There was no treatment effect on the levels of protein, glucose, urea or non-esterified fatty acids in the blood of the goats.
31113503	3	50	theme	Thirty-six	946:955	arg1	goats					971:975	Thirty-six mid-lactation goats	946:975	Thirty-six mid-lactation goats	946:975	Thirty-six mid-lactation goats were housed indoors in pairs and split into two groups (A and B).
31113503	8	51	theme	urea	1686:1689	arg1	levels					1658:1663	the levels	1654:1663	the levels of protein, glucose, urea or non-esterified fatty acids	1654:1719	There was no treatment effect on the levels of protein, glucose, urea or non-esterified fatty acids in the blood of the goats.
31113503	4	52	theme	uniformity	1094:1103	arg1	period					1105:1110	firstly a uniformity period	1084:1110	firstly a uniformity period of 6 days	1084:1120	The trial was split into three periods - firstly a uniformity period of 6 days, in which all goats were fed a combination of both green fodder types, followed by two treatment periods (P1 and P2) of 12 days, respectively.
31113503	2	53	theme	white	914:918	arg1	SF					941:942	SF	941:942	SF	941:942	The aim of this experiment was to determine whether feeding lactating dairy goats a mixed-species green fodder (MF, consisting of perennial ryegrass, timothy (Phleum pratense L.), prairie grass (Bromus willdenowii Kunth), white clover, red clover (Trifolium pratense L.), lucerne (Medicago sativa L.), chicory (Cichorium intybus L.) and plantain (Plantago lanceolata L.) improves dietary intake, milk yield and composition compared with a standard ryegrass and white clover green fodder (SF).
31113503	2	53	theme	white	914:918	arg1	fodder					933:938	white clover green fodder	914:938	white clover green fodder (SF)	914:943	The aim of this experiment was to determine whether feeding lactating dairy goats a mixed-species green fodder (MF, consisting of perennial ryegrass, timothy (Phleum pratense L.), prairie grass (Bromus willdenowii Kunth), white clover, red clover (Trifolium pratense L.), lucerne (Medicago sativa L.), chicory (Cichorium intybus L.) and plantain (Plantago lanceolata L.) improves dietary intake, milk yield and composition compared with a standard ryegrass and white clover green fodder (SF).
31113503	1	54	theme	green	439:443	arg1	fodder					445:450	the green fodder	435:450	the green fodder	435:450	The majority of New Zealand dairy goat farmers utilise cultivated green-fed fodder dominated by perennial ryegrass (Lolium perenne L.) and white clover (Trifolium repens L.), but evidence from other ruminant species suggests that milk production may be improved when using a more diverse array of species within the green fodder.
31113503	9	55	theme	cis-vaccenic	1864:1875	arg1	acid					1877:1880	cis-vaccenic acid	1864:1880	cis-vaccenic acid (C18:1 c11)	1864:1892	An effect of green fodder type on milk fat profile was demonstrated, with proportions of pentadecylic acid (C15:0), cis-vaccenic acid (C18:1 c11), linoleic acid (C18:2 n6) and α-linolenic acid (C18:3 n3) being increased in response to MF consumption.
31113503	9	55	theme	cis-vaccenic	1864:1875	arg1	c11					1889:1891	C18:1 c11	1883:1891	C18:1 c11	1883:1891	An effect of green fodder type on milk fat profile was demonstrated, with proportions of pentadecylic acid (C15:0), cis-vaccenic acid (C18:1 c11), linoleic acid (C18:2 n6) and α-linolenic acid (C18:3 n3) being increased in response to MF consumption.
31113503	7	56	theme	milk	1472:1475	arg1	protein					1477:1483	the milk protein	1468:1483	the milk protein	1468:1483	In addition, the milk protein and fat concentration of goats fed MF were 4% greater than for those fed SF, whereas there was no effect on milk lactose concentration.
31113503	8	57	theme	non-esterified	1694:1707	arg1	acids					1715:1719	non-esterified fatty acids	1694:1719	non-esterified fatty acids	1694:1719	There was no treatment effect on the levels of protein, glucose, urea or non-esterified fatty acids in the blood of the goats.
31113503	2	58	theme	pratense	711:718	arg1	clover					693:698	red clover	689:698	red clover (Trifolium pratense L.)	689:722	The aim of this experiment was to determine whether feeding lactating dairy goats a mixed-species green fodder (MF, consisting of perennial ryegrass, timothy (Phleum pratense L.), prairie grass (Bromus willdenowii Kunth), white clover, red clover (Trifolium pratense L.), lucerne (Medicago sativa L.), chicory (Cichorium intybus L.) and plantain (Plantago lanceolata L.) improves dietary intake, milk yield and composition compared with a standard ryegrass and white clover green fodder (SF).
31113503	2	58	theme	pratense	711:718	arg1	L.					720:721	Trifolium pratense L.	701:721	Trifolium pratense L.	701:721	The aim of this experiment was to determine whether feeding lactating dairy goats a mixed-species green fodder (MF, consisting of perennial ryegrass, timothy (Phleum pratense L.), prairie grass (Bromus willdenowii Kunth), white clover, red clover (Trifolium pratense L.), lucerne (Medicago sativa L.), chicory (Cichorium intybus L.) and plantain (Plantago lanceolata L.) improves dietary intake, milk yield and composition compared with a standard ryegrass and white clover green fodder (SF).
31113503	6	59	theme	greater	1417:1423	arg1	yield					1430:1434	7% greater milk yield	1414:1434	7% greater milk yield	1414:1434	Goats fed MF had 13% greater dry matter intake and 7% greater milk yield than goats fed SF.
31113503	8	60	theme	acids	1715:1719	arg1	levels					1658:1663	the levels	1654:1663	the levels of protein, glucose, urea or non-esterified fatty acids	1654:1719	There was no treatment effect on the levels of protein, glucose, urea or non-esterified fatty acids in the blood of the goats.
31113503	12	61	theme	α-linolenic	2351:2361	arg1	acids					2363:2367	linoleic and α-linolenic acids	2338:2367	linoleic and α-linolenic acids	2338:2367	The green fodder type affected the fatty acid profile of goat's milk, with MF increasing the levels of beneficial polyunsaturated omega fatty acids (linoleic and α-linolenic acids).
31113503	12	61	theme	α-linolenic	2351:2361	arg1	acids					2331:2335	beneficial polyunsaturated omega fatty acids	2292:2335	beneficial polyunsaturated omega fatty acids (linoleic and α-linolenic acids)	2292:2368	The green fodder type affected the fatty acid profile of goat's milk, with MF increasing the levels of beneficial polyunsaturated omega fatty acids (linoleic and α-linolenic acids).
31113503	1	62	theme	perennial	219:227	arg1	ryegrass					229:236	perennial ryegrass	219:236	perennial ryegrass (Lolium perenne L.)	219:256	The majority of New Zealand dairy goat farmers utilise cultivated green-fed fodder dominated by perennial ryegrass (Lolium perenne L.) and white clover (Trifolium repens L.), but evidence from other ruminant species suggests that milk production may be improved when using a more diverse array of species within the green fodder.
31113503	1	62	theme	perennial	219:227	arg1	L.					254:255	L.	254:255	L.	254:255	The majority of New Zealand dairy goat farmers utilise cultivated green-fed fodder dominated by perennial ryegrass (Lolium perenne L.) and white clover (Trifolium repens L.), but evidence from other ruminant species suggests that milk production may be improved when using a more diverse array of species within the green fodder.
31113503	2	63	theme	mixed-species	537:549	arg1	fodder					557:562	a mixed-species green fodder	535:562	lactating dairy goats a mixed-species green fodder (MF, consisting of perennial ryegrass, timothy (Phleum pratense L.), prairie grass (Bromus willdenowii Kunth), white clover, red clover (Trifolium pratense L.), lucerne (Medicago sativa L.), chicory (Cichorium intybus L.) and plantain (Plantago lanceolata L.) improves dietary intake, milk yield and composition compared with a standard ryegrass and white clover green fodder (SF)	513:943	The aim of this experiment was to determine whether feeding lactating dairy goats a mixed-species green fodder (MF, consisting of perennial ryegrass, timothy (Phleum pratense L.), prairie grass (Bromus willdenowii Kunth), white clover, red clover (Trifolium pratense L.), lucerne (Medicago sativa L.), chicory (Cichorium intybus L.) and plantain (Plantago lanceolata L.) improves dietary intake, milk yield and composition compared with a standard ryegrass and white clover green fodder (SF).
31113503	9	64	theme	acid	1904:1907	arg1	proportions					1822:1832	proportions	1822:1832	proportions of pentadecylic acid (C15:0), cis-vaccenic acid (C18:1 c11), linoleic acid (C18:2 n6) and α-linolenic acid (C18:3 n3)	1822:1950	An effect of green fodder type on milk fat profile was demonstrated, with proportions of pentadecylic acid (C15:0), cis-vaccenic acid (C18:1 c11), linoleic acid (C18:2 n6) and α-linolenic acid (C18:3 n3) being increased in response to MF consumption.
31113503	2	65	dep	lucerne	725:731	arg1	Medicago					734:741	Medicago	734:741	Medicago	734:741	The aim of this experiment was to determine whether feeding lactating dairy goats a mixed-species green fodder (MF, consisting of perennial ryegrass, timothy (Phleum pratense L.), prairie grass (Bromus willdenowii Kunth), white clover, red clover (Trifolium pratense L.), lucerne (Medicago sativa L.), chicory (Cichorium intybus L.) and plantain (Plantago lanceolata L.) improves dietary intake, milk yield and composition compared with a standard ryegrass and white clover green fodder (SF).
31113503	8	66	theme	goats	1741:1745	arg1	blood					1728:1732	the blood	1724:1732	the blood of the goats	1724:1745	There was no treatment effect on the levels of protein, glucose, urea or non-esterified fatty acids in the blood of the goats.
31113503	2	67	dep	Cichorium	764:772	arg1	L.					782:783	Cichorium intybus L.	764:783	Cichorium intybus L.	764:783	The aim of this experiment was to determine whether feeding lactating dairy goats a mixed-species green fodder (MF, consisting of perennial ryegrass, timothy (Phleum pratense L.), prairie grass (Bromus willdenowii Kunth), white clover, red clover (Trifolium pratense L.), lucerne (Medicago sativa L.), chicory (Cichorium intybus L.) and plantain (Plantago lanceolata L.) improves dietary intake, milk yield and composition compared with a standard ryegrass and white clover green fodder (SF).
31113503	1	68	theme	diverse	403:409	arg1	array					411:415	a more diverse array	396:415	a more diverse array of species within the green fodder	396:450	The majority of New Zealand dairy goat farmers utilise cultivated green-fed fodder dominated by perennial ryegrass (Lolium perenne L.) and white clover (Trifolium repens L.), but evidence from other ruminant species suggests that milk production may be improved when using a more diverse array of species within the green fodder.
31113503	1	69	theme	Zealand	143:149	arg1	farmers					162:168	New Zealand dairy goat farmers	139:168	New Zealand dairy goat farmers	139:168	The majority of New Zealand dairy goat farmers utilise cultivated green-fed fodder dominated by perennial ryegrass (Lolium perenne L.) and white clover (Trifolium repens L.), but evidence from other ruminant species suggests that milk production may be improved when using a more diverse array of species within the green fodder.
31113503	5	70	theme	group	1329:1333	arg1	diets					1335:1339	then the group diets	1320:1339	then the group diets	1320:1339	For P1, group A was fed MF and group B was fed SF, and then the group diets were switched for P2.
31113503	4	71	theme	days	1117:1120	arg1	period					1105:1110	firstly a uniformity period	1084:1110	firstly a uniformity period of 6 days	1084:1120	The trial was split into three periods - firstly a uniformity period of 6 days, in which all goats were fed a combination of both green fodder types, followed by two treatment periods (P1 and P2) of 12 days, respectively.
31113503	1	72	theme	goat	157:160	arg1	farmers					162:168	New Zealand dairy goat farmers	139:168	New Zealand dairy goat farmers	139:168	The majority of New Zealand dairy goat farmers utilise cultivated green-fed fodder dominated by perennial ryegrass (Lolium perenne L.) and white clover (Trifolium repens L.), but evidence from other ruminant species suggests that milk production may be improved when using a more diverse array of species within the green fodder.
31113503	2	73	dep	fodder	557:562	arg1	improves					824:831	improves	824:831	improves dietary intake, milk yield and composition compared with a standard ryegrass and white clover green fodder (SF)	824:943	The aim of this experiment was to determine whether feeding lactating dairy goats a mixed-species green fodder (MF, consisting of perennial ryegrass, timothy (Phleum pratense L.), prairie grass (Bromus willdenowii Kunth), white clover, red clover (Trifolium pratense L.), lucerne (Medicago sativa L.), chicory (Cichorium intybus L.) and plantain (Plantago lanceolata L.) improves dietary intake, milk yield and composition compared with a standard ryegrass and white clover green fodder (SF).
31113503	2	74	theme	red	689:691	arg1	clover					693:698	red clover	689:698	red clover (Trifolium pratense L.)	689:722	The aim of this experiment was to determine whether feeding lactating dairy goats a mixed-species green fodder (MF, consisting of perennial ryegrass, timothy (Phleum pratense L.), prairie grass (Bromus willdenowii Kunth), white clover, red clover (Trifolium pratense L.), lucerne (Medicago sativa L.), chicory (Cichorium intybus L.) and plantain (Plantago lanceolata L.) improves dietary intake, milk yield and composition compared with a standard ryegrass and white clover green fodder (SF).
31113503	2	74	theme	red	689:691	arg1	L.					720:721	Trifolium pratense L.	701:721	Trifolium pratense L.	701:721	The aim of this experiment was to determine whether feeding lactating dairy goats a mixed-species green fodder (MF, consisting of perennial ryegrass, timothy (Phleum pratense L.), prairie grass (Bromus willdenowii Kunth), white clover, red clover (Trifolium pratense L.), lucerne (Medicago sativa L.), chicory (Cichorium intybus L.) and plantain (Plantago lanceolata L.) improves dietary intake, milk yield and composition compared with a standard ryegrass and white clover green fodder (SF).
31113503	0	75	theme	dairy	110:114	arg1	goats					116:120	housed dairy goats	103:120	housed dairy goats	103:120	The effect of cultivated mixed-species green fodder on intake, milk production and milk composition of housed dairy goats.
31113503	9	76	theme	α-linolenic	1924:1934	arg1	n3					1948:1949	C18:3 n3	1942:1949	C18:3 n3	1942:1949	An effect of green fodder type on milk fat profile was demonstrated, with proportions of pentadecylic acid (C15:0), cis-vaccenic acid (C18:1 c11), linoleic acid (C18:2 n6) and α-linolenic acid (C18:3 n3) being increased in response to MF consumption.
31113503	9	76	theme	α-linolenic	1924:1934	arg1	acid					1936:1939	α-linolenic acid	1924:1939	α-linolenic acid (C18:3 n3)	1924:1950	An effect of green fodder type on milk fat profile was demonstrated, with proportions of pentadecylic acid (C15:0), cis-vaccenic acid (C18:1 c11), linoleic acid (C18:2 n6) and α-linolenic acid (C18:3 n3) being increased in response to MF consumption.
31113503	7	77	theme	milk	1593:1596	arg1	concentration					1606:1618	milk lactose concentration	1593:1618	milk lactose concentration	1593:1618	In addition, the milk protein and fat concentration of goats fed MF were 4% greater than for those fed SF, whereas there was no effect on milk lactose concentration.
31113503	1	78	theme	green-fed	189:197	arg1	fodder					199:204	cultivated green-fed fodder	178:204	cultivated green-fed fodder dominated by perennial ryegrass (Lolium perenne L.) and white clover (Trifolium repens L.)	178:295	The majority of New Zealand dairy goat farmers utilise cultivated green-fed fodder dominated by perennial ryegrass (Lolium perenne L.) and white clover (Trifolium repens L.), but evidence from other ruminant species suggests that milk production may be improved when using a more diverse array of species within the green fodder.
31113503	2	79	dep	goats	529:533	arg1	fodder					557:562	a mixed-species green fodder	535:562	lactating dairy goats a mixed-species green fodder (MF, consisting of perennial ryegrass, timothy (Phleum pratense L.), prairie grass (Bromus willdenowii Kunth), white clover, red clover (Trifolium pratense L.), lucerne (Medicago sativa L.), chicory (Cichorium intybus L.) and plantain (Plantago lanceolata L.) improves dietary intake, milk yield and composition compared with a standard ryegrass and white clover green fodder (SF)	513:943	The aim of this experiment was to determine whether feeding lactating dairy goats a mixed-species green fodder (MF, consisting of perennial ryegrass, timothy (Phleum pratense L.), prairie grass (Bromus willdenowii Kunth), white clover, red clover (Trifolium pratense L.), lucerne (Medicago sativa L.), chicory (Cichorium intybus L.) and plantain (Plantago lanceolata L.) improves dietary intake, milk yield and composition compared with a standard ryegrass and white clover green fodder (SF).
31113503	2	80	theme	experiment	469:478	arg1	aim					457:459	The aim	453:459	The aim of this experiment	453:478	The aim of this experiment was to determine whether feeding lactating dairy goats a mixed-species green fodder (MF, consisting of perennial ryegrass, timothy (Phleum pratense L.), prairie grass (Bromus willdenowii Kunth), white clover, red clover (Trifolium pratense L.), lucerne (Medicago sativa L.), chicory (Cichorium intybus L.) and plantain (Plantago lanceolata L.) improves dietary intake, milk yield and composition compared with a standard ryegrass and white clover green fodder (SF).
31113503	11	81	theme	milk	2145:2148	arg1	production					2150:2159	milk production	2145:2159	milk production	2145:2159	In summary, this study demonstrated that goats fed MF increased green fodder intake and milk production compared with goats fed SF.
31113503	9	82	theme	green	1761:1765	arg1	type					1774:1777	green fodder type	1761:1777	green fodder type	1761:1777	An effect of green fodder type on milk fat profile was demonstrated, with proportions of pentadecylic acid (C15:0), cis-vaccenic acid (C18:1 c11), linoleic acid (C18:2 n6) and α-linolenic acid (C18:3 n3) being increased in response to MF consumption.
31113503	2	83	theme	lactating	513:521	arg1	goats					529:533	lactating dairy goats a mixed-species green fodder (MF, consisting of perennial ryegrass, timothy (Phleum pratense L.), prairie grass (Bromus willdenowii Kunth), white clover, red clover (Trifolium pratense L.), lucerne (Medicago sativa L.), chicory (Cichorium intybus L.) and plantain (Plantago lanceolata L.) improves dietary intake, milk yield and composition compared with a standard ryegrass and white clover green fodder (SF)	513:943	lactating dairy goats a mixed-species green fodder (MF, consisting of perennial ryegrass, timothy (Phleum pratense L.), prairie grass (Bromus willdenowii Kunth), white clover, red clover (Trifolium pratense L.), lucerne (Medicago sativa L.), chicory (Cichorium intybus L.) and plantain (Plantago lanceolata L.) improves dietary intake, milk yield and composition compared with a standard ryegrass and white clover green fodder (SF)	513:943	The aim of this experiment was to determine whether feeding lactating dairy goats a mixed-species green fodder (MF, consisting of perennial ryegrass, timothy (Phleum pratense L.), prairie grass (Bromus willdenowii Kunth), white clover, red clover (Trifolium pratense L.), lucerne (Medicago sativa L.), chicory (Cichorium intybus L.) and plantain (Plantago lanceolata L.) improves dietary intake, milk yield and composition compared with a standard ryegrass and white clover green fodder (SF).
31113503	9	84	theme	type	1774:1777	arg1	effect					1751:1756	An effect	1748:1756	An effect of green fodder type on milk fat profile	1748:1797	An effect of green fodder type on milk fat profile was demonstrated, with proportions of pentadecylic acid (C15:0), cis-vaccenic acid (C18:1 c11), linoleic acid (C18:2 n6) and α-linolenic acid (C18:3 n3) being increased in response to MF consumption.
31113503	9	85	theme	milk	1782:1785	arg1	fat					1787:1789	milk fat	1782:1789	milk fat profile	1782:1797	An effect of green fodder type on milk fat profile was demonstrated, with proportions of pentadecylic acid (C15:0), cis-vaccenic acid (C18:1 c11), linoleic acid (C18:2 n6) and α-linolenic acid (C18:3 n3) being increased in response to MF consumption.
31113503	12	86	theme	fatty	2325:2329	arg1	acids					2363:2367	linoleic and α-linolenic acids	2338:2367	linoleic and α-linolenic acids	2338:2367	The green fodder type affected the fatty acid profile of goat's milk, with MF increasing the levels of beneficial polyunsaturated omega fatty acids (linoleic and α-linolenic acids).
31113503	12	86	theme	fatty	2325:2329	arg1	acids					2331:2335	beneficial polyunsaturated omega fatty acids	2292:2335	beneficial polyunsaturated omega fatty acids (linoleic and α-linolenic acids)	2292:2368	The green fodder type affected the fatty acid profile of goat's milk, with MF increasing the levels of beneficial polyunsaturated omega fatty acids (linoleic and α-linolenic acids).
31113503	1	87	dep	repens	286:291	arg1	L.					293:294	L.	293:294	L.	293:294	The majority of New Zealand dairy goat farmers utilise cultivated green-fed fodder dominated by perennial ryegrass (Lolium perenne L.) and white clover (Trifolium repens L.), but evidence from other ruminant species suggests that milk production may be improved when using a more diverse array of species within the green fodder.
31113503	4	88	theme	types	1186:1190	arg1	combination					1153:1163	a combination	1151:1163	a combination	1151:1163	The trial was split into three periods - firstly a uniformity period of 6 days, in which all goats were fed a combination of both green fodder types, followed by two treatment periods (P1 and P2) of 12 days, respectively.
31113503	0	89	theme	milk	83:86	arg1	composition					88:98	milk composition	83:98	milk composition	83:98	The effect of cultivated mixed-species green fodder on intake, milk production and milk composition of housed dairy goats.
31113503	7	90	theme	fed	1554:1556	arg1	SF					1558:1559	those fed SF	1548:1559	those fed SF	1548:1559	In addition, the milk protein and fat concentration of goats fed MF were 4% greater than for those fed SF, whereas there was no effect on milk lactose concentration.
31113503	2	91	theme	milk	849:852	arg1	yield					854:858	milk yield	849:858	milk yield	849:858	The aim of this experiment was to determine whether feeding lactating dairy goats a mixed-species green fodder (MF, consisting of perennial ryegrass, timothy (Phleum pratense L.), prairie grass (Bromus willdenowii Kunth), white clover, red clover (Trifolium pratense L.), lucerne (Medicago sativa L.), chicory (Cichorium intybus L.) and plantain (Plantago lanceolata L.) improves dietary intake, milk yield and composition compared with a standard ryegrass and white clover green fodder (SF).
31113503	12	92	theme	acid	2230:2233	arg1	profile					2235:2241	the fatty acid profile	2220:2241	the fatty acid profile of goat's milk	2220:2256	The green fodder type affected the fatty acid profile of goat's milk, with MF increasing the levels of beneficial polyunsaturated omega fatty acids (linoleic and α-linolenic acids).
31113503	1	93	theme	other	316:320	arg1	species					331:337	other ruminant species	316:337	other ruminant species	316:337	The majority of New Zealand dairy goat farmers utilise cultivated green-fed fodder dominated by perennial ryegrass (Lolium perenne L.) and white clover (Trifolium repens L.), but evidence from other ruminant species suggests that milk production may be improved when using a more diverse array of species within the green fodder.
31113503	12	94	theme	polyunsaturated	2303:2317	arg1	acids					2363:2367	linoleic and α-linolenic acids	2338:2367	linoleic and α-linolenic acids	2338:2367	The green fodder type affected the fatty acid profile of goat's milk, with MF increasing the levels of beneficial polyunsaturated omega fatty acids (linoleic and α-linolenic acids).
31113503	12	94	theme	polyunsaturated	2303:2317	arg1	acids					2331:2335	beneficial polyunsaturated omega fatty acids	2292:2335	beneficial polyunsaturated omega fatty acids (linoleic and α-linolenic acids)	2292:2368	The green fodder type affected the fatty acid profile of goat's milk, with MF increasing the levels of beneficial polyunsaturated omega fatty acids (linoleic and α-linolenic acids).
31113503	6	95	theme	greater	1384:1390	arg1	intake					1403:1408	13% greater dry matter intake	1380:1408	13% greater dry matter intake	1380:1408	Goats fed MF had 13% greater dry matter intake and 7% greater milk yield than goats fed SF.
31113503	2	96	theme	Phleum	612:617	arg1	timothy					603:609	timothy	603:609	timothy (Phleum pratense L.)	603:630	The aim of this experiment was to determine whether feeding lactating dairy goats a mixed-species green fodder (MF, consisting of perennial ryegrass, timothy (Phleum pratense L.), prairie grass (Bromus willdenowii Kunth), white clover, red clover (Trifolium pratense L.), lucerne (Medicago sativa L.), chicory (Cichorium intybus L.) and plantain (Plantago lanceolata L.) improves dietary intake, milk yield and composition compared with a standard ryegrass and white clover green fodder (SF).
31113503	2	96	theme	Phleum	612:617	arg1	L.					628:629	Phleum pratense L.	612:629	Phleum pratense L.	612:629	The aim of this experiment was to determine whether feeding lactating dairy goats a mixed-species green fodder (MF, consisting of perennial ryegrass, timothy (Phleum pratense L.), prairie grass (Bromus willdenowii Kunth), white clover, red clover (Trifolium pratense L.), lucerne (Medicago sativa L.), chicory (Cichorium intybus L.) and plantain (Plantago lanceolata L.) improves dietary intake, milk yield and composition compared with a standard ryegrass and white clover green fodder (SF).
31113503	8	97	from	effect	1644:1649	arg1	levels					1658:1663	the levels	1654:1663	the levels of protein, glucose, urea or non-esterified fatty acids	1654:1719	There was no treatment effect on the levels of protein, glucose, urea or non-esterified fatty acids in the blood of the goats.
31113503	0	98	from	fodder	45:50	arg1	intake					55:60	intake	55:60	intake	55:60	The effect of cultivated mixed-species green fodder on intake, milk production and milk composition of housed dairy goats.
31113503	0	98	from	fodder	45:50	arg1	production					68:77	milk production	63:77	milk production	63:77	The effect of cultivated mixed-species green fodder on intake, milk production and milk composition of housed dairy goats.
31113503	0	98	from	fodder	45:50	arg1	composition					88:98	milk composition	83:98	milk composition	83:98	The effect of cultivated mixed-species green fodder on intake, milk production and milk composition of housed dairy goats.
31113503	11	99	theme	fodder	2127:2132	arg1	intake					2134:2139	green fodder intake	2121:2139	green fodder intake	2121:2139	In summary, this study demonstrated that goats fed MF increased green fodder intake and milk production compared with goats fed SF.
31113503	4	100	theme	days	1245:1248	arg1	P2					1235:1236	P2	1235:1236	P2	1235:1236	The trial was split into three periods - firstly a uniformity period of 6 days, in which all goats were fed a combination of both green fodder types, followed by two treatment periods (P1 and P2) of 12 days, respectively.
31113503	4	100	theme	days	1245:1248	arg1	P1					1228:1229	P1	1228:1229	P1	1228:1229	The trial was split into three periods - firstly a uniformity period of 6 days, in which all goats were fed a combination of both green fodder types, followed by two treatment periods (P1 and P2) of 12 days, respectively.
31113503	4	100	theme	days	1245:1248	arg1	periods					1219:1225	two treatment periods	1205:1225	two treatment periods (P1 and P2)	1205:1237	The trial was split into three periods - firstly a uniformity period of 6 days, in which all goats were fed a combination of both green fodder types, followed by two treatment periods (P1 and P2) of 12 days, respectively.
31113503	0	101	theme	cultivated	14:23	arg1	effect					4:9	The effect	0:9	The effect of cultivated	0:23	The effect of cultivated mixed-species green fodder on intake, milk production and milk composition of housed dairy goats.
31113503	3	102	theme	mid-lactation	957:969	arg1	goats					971:975	Thirty-six mid-lactation goats	946:975	Thirty-six mid-lactation goats	946:975	Thirty-six mid-lactation goats were housed indoors in pairs and split into two groups (A and B).
31113503	5	103	theme	group	1273:1277	arg1	A					1279:1279	group A	1273:1279	group A	1273:1279	For P1, group A was fed MF and group B was fed SF, and then the group diets were switched for P2.
31113503	0	104	theme	green	39:43	arg1	fodder					45:50	green fodder	39:50	green fodder on intake, milk production and milk composition of housed dairy goats	39:120	The effect of cultivated mixed-species green fodder on intake, milk production and milk composition of housed dairy goats.
31113503	2	105	theme	clover	920:925	arg1	SF					941:942	SF	941:942	SF	941:942	The aim of this experiment was to determine whether feeding lactating dairy goats a mixed-species green fodder (MF, consisting of perennial ryegrass, timothy (Phleum pratense L.), prairie grass (Bromus willdenowii Kunth), white clover, red clover (Trifolium pratense L.), lucerne (Medicago sativa L.), chicory (Cichorium intybus L.) and plantain (Plantago lanceolata L.) improves dietary intake, milk yield and composition compared with a standard ryegrass and white clover green fodder (SF).
31113503	2	105	theme	clover	920:925	arg1	fodder					933:938	white clover green fodder	914:938	white clover green fodder (SF)	914:943	The aim of this experiment was to determine whether feeding lactating dairy goats a mixed-species green fodder (MF, consisting of perennial ryegrass, timothy (Phleum pratense L.), prairie grass (Bromus willdenowii Kunth), white clover, red clover (Trifolium pratense L.), lucerne (Medicago sativa L.), chicory (Cichorium intybus L.) and plantain (Plantago lanceolata L.) improves dietary intake, milk yield and composition compared with a standard ryegrass and white clover green fodder (SF).
31113503	1	106	theme	Trifolium	276:284	arg1	clover					268:273	white clover	262:273	white clover (Trifolium repens L.)	262:295	The majority of New Zealand dairy goat farmers utilise cultivated green-fed fodder dominated by perennial ryegrass (Lolium perenne L.) and white clover (Trifolium repens L.), but evidence from other ruminant species suggests that milk production may be improved when using a more diverse array of species within the green fodder.
31113503	1	106	theme	Trifolium	276:284	arg1	repens					286:291	Trifolium repens L.	276:294	Trifolium repens L.	276:294	The majority of New Zealand dairy goat farmers utilise cultivated green-fed fodder dominated by perennial ryegrass (Lolium perenne L.) and white clover (Trifolium repens L.), but evidence from other ruminant species suggests that milk production may be improved when using a more diverse array of species within the green fodder.
31113503	7	107	theme	goats	1510:1514	arg1	concentration					1493:1505	the milk protein and fat concentration	1468:1505	the milk protein and fat concentration of goats fed MF	1468:1521	In addition, the milk protein and fat concentration of goats fed MF were 4% greater than for those fed SF, whereas there was no effect on milk lactose concentration.
31113503	7	108	theme	fat	1489:1491	arg1	concentration					1493:1505	the milk protein and fat concentration	1468:1505	the milk protein and fat concentration of goats fed MF	1468:1521	In addition, the milk protein and fat concentration of goats fed MF were 4% greater than for those fed SF, whereas there was no effect on milk lactose concentration.
31113503	8	109	theme	fatty	1709:1713	arg1	acids					1715:1719	non-esterified fatty acids	1694:1719	non-esterified fatty acids	1694:1719	There was no treatment effect on the levels of protein, glucose, urea or non-esterified fatty acids in the blood of the goats.
31113503	12	110	theme	green	2193:2197	arg1	type					2206:2209	The green fodder type	2189:2209	The green fodder type	2189:2209	The green fodder type affected the fatty acid profile of goat's milk, with MF increasing the levels of beneficial polyunsaturated omega fatty acids (linoleic and α-linolenic acids).
31113503	6	111	theme	milk	1425:1428	arg1	yield					1430:1434	7% greater milk yield	1414:1434	7% greater milk yield	1414:1434	Goats fed MF had 13% greater dry matter intake and 7% greater milk yield than goats fed SF.
31113503	2	112	dep	Medicago	734:741	arg1	L.					750:751	sativa L.	743:751	sativa L.	743:751	The aim of this experiment was to determine whether feeding lactating dairy goats a mixed-species green fodder (MF, consisting of perennial ryegrass, timothy (Phleum pratense L.), prairie grass (Bromus willdenowii Kunth), white clover, red clover (Trifolium pratense L.), lucerne (Medicago sativa L.), chicory (Cichorium intybus L.) and plantain (Plantago lanceolata L.) improves dietary intake, milk yield and composition compared with a standard ryegrass and white clover green fodder (SF).
31113503	9	113	theme	acid	1877:1880	arg1	proportions					1822:1832	proportions	1822:1832	proportions of pentadecylic acid (C15:0), cis-vaccenic acid (C18:1 c11), linoleic acid (C18:2 n6) and α-linolenic acid (C18:3 n3)	1822:1950	An effect of green fodder type on milk fat profile was demonstrated, with proportions of pentadecylic acid (C15:0), cis-vaccenic acid (C18:1 c11), linoleic acid (C18:2 n6) and α-linolenic acid (C18:3 n3) being increased in response to MF consumption.
31113503	4	114	dep	periods	1219:1225	arg1	P2					1235:1236	P2	1235:1236	P2	1235:1236	The trial was split into three periods - firstly a uniformity period of 6 days, in which all goats were fed a combination of both green fodder types, followed by two treatment periods (P1 and P2) of 12 days, respectively.
31113503	4	114	dep	periods	1219:1225	arg1	P1					1228:1229	P1	1228:1229	P1	1228:1229	The trial was split into three periods - firstly a uniformity period of 6 days, in which all goats were fed a combination of both green fodder types, followed by two treatment periods (P1 and P2) of 12 days, respectively.
31113503	4	114	dep	periods	1219:1225	arg1	periods					1219:1225	two treatment periods	1205:1225	two treatment periods (P1 and P2)	1205:1237	The trial was split into three periods - firstly a uniformity period of 6 days, in which all goats were fed a combination of both green fodder types, followed by two treatment periods (P1 and P2) of 12 days, respectively.
31113503	7	115	theme	protein	1477:1483	arg1	concentration					1493:1505	the milk protein and fat concentration	1468:1505	the milk protein and fat concentration of goats fed MF	1468:1521	In addition, the milk protein and fat concentration of goats fed MF were 4% greater than for those fed SF, whereas there was no effect on milk lactose concentration.
31113503	6	116	contain	had	1376:1378	arg1	Goats					1363:1367	Goats	1363:1367	Goats fed MF	1363:1374	Goats fed MF had 13% greater dry matter intake and 7% greater milk yield than goats fed SF.
31113503	6	116	contain	had	1376:1378	arg2	intake					1403:1408	13% greater dry matter intake	1380:1408	13% greater dry matter intake	1380:1408	Goats fed MF had 13% greater dry matter intake and 7% greater milk yield than goats fed SF.
31113503	6	116	contain	had	1376:1378	arg2	yield					1430:1434	7% greater milk yield	1414:1434	7% greater milk yield	1414:1434	Goats fed MF had 13% greater dry matter intake and 7% greater milk yield than goats fed SF.
31113503	2	117	theme	Trifolium	701:709	arg1	clover					693:698	red clover	689:698	red clover (Trifolium pratense L.)	689:722	The aim of this experiment was to determine whether feeding lactating dairy goats a mixed-species green fodder (MF, consisting of perennial ryegrass, timothy (Phleum pratense L.), prairie grass (Bromus willdenowii Kunth), white clover, red clover (Trifolium pratense L.), lucerne (Medicago sativa L.), chicory (Cichorium intybus L.) and plantain (Plantago lanceolata L.) improves dietary intake, milk yield and composition compared with a standard ryegrass and white clover green fodder (SF).
31113503	2	117	theme	Trifolium	701:709	arg1	L.					720:721	Trifolium pratense L.	701:721	Trifolium pratense L.	701:721	The aim of this experiment was to determine whether feeding lactating dairy goats a mixed-species green fodder (MF, consisting of perennial ryegrass, timothy (Phleum pratense L.), prairie grass (Bromus willdenowii Kunth), white clover, red clover (Trifolium pratense L.), lucerne (Medicago sativa L.), chicory (Cichorium intybus L.) and plantain (Plantago lanceolata L.) improves dietary intake, milk yield and composition compared with a standard ryegrass and white clover green fodder (SF).
31113503	9	118	theme	C18:1	1883:1887	arg1	acid					1877:1880	cis-vaccenic acid	1864:1880	cis-vaccenic acid (C18:1 c11)	1864:1892	An effect of green fodder type on milk fat profile was demonstrated, with proportions of pentadecylic acid (C15:0), cis-vaccenic acid (C18:1 c11), linoleic acid (C18:2 n6) and α-linolenic acid (C18:3 n3) being increased in response to MF consumption.
31113503	9	118	theme	C18:1	1883:1887	arg1	c11					1889:1891	C18:1 c11	1883:1891	C18:1 c11	1883:1891	An effect of green fodder type on milk fat profile was demonstrated, with proportions of pentadecylic acid (C15:0), cis-vaccenic acid (C18:1 c11), linoleic acid (C18:2 n6) and α-linolenic acid (C18:3 n3) being increased in response to MF consumption.
31113503	6	119	theme	matter	1396:1401	arg1	intake					1403:1408	13% greater dry matter intake	1380:1408	13% greater dry matter intake	1380:1408	Goats fed MF had 13% greater dry matter intake and 7% greater milk yield than goats fed SF.
31316128	5	0	theme	dietary	1084:1090	arg1	fiber					1092:1096	unknown dietary fiber	1076:1096	unknown dietary fiber	1076:1096	A single 100-g serving of fresh organic kale can provide mineral micronutrients (43-438 mg Ca; 11-60 mg Mg; 28-102 mg P; 0.5-3.3 mg Fe; 0.3-1.3 mg Mn; 1-136 µg Cu; and 0-35 µg Se) as well as 5.7-8.7 g of total prebiotic carbohydrates, including sugar alcohols (0.4-6.6 mg), simple sugars (6-1507 mg), raffinose and fructooligosaccharides (0.8-169 mg), hemicellulose (77-763 mg), lignin (0-90 mg), and unknown dietary fiber (5-6 g).
31316128	7	1	theme	composition	1345:1355	arg1	losses					1357:1362	considerable biomass and nutrient composition losses	1311:1362	considerable biomass and nutrient composition losses	1311:1362	Study results indicate that Starbor and Red Russian are the most suitable kale cultivars for organic production without considerable biomass and nutrient composition losses.
31316128	7	2	theme	organic	1284:1290	arg1	production					1292:1301	organic production	1284:1301	organic production without considerable biomass and nutrient composition losses	1284:1362	Study results indicate that Starbor and Red Russian are the most suitable kale cultivars for organic production without considerable biomass and nutrient composition losses.
31316128	5	3	theme	unknown	1076:1082	arg1	fiber					1092:1096	unknown dietary fiber	1076:1096	unknown dietary fiber	1076:1096	A single 100-g serving of fresh organic kale can provide mineral micronutrients (43-438 mg Ca; 11-60 mg Mg; 28-102 mg P; 0.5-3.3 mg Fe; 0.3-1.3 mg Mn; 1-136 µg Cu; and 0-35 µg Se) as well as 5.7-8.7 g of total prebiotic carbohydrates, including sugar alcohols (0.4-6.6 mg), simple sugars (6-1507 mg), raffinose and fructooligosaccharides (0.8-169 mg), hemicellulose (77-763 mg), lignin (0-90 mg), and unknown dietary fiber (5-6 g).
31316128	3	4	theme	recent	207:212	arg1	years					214:218	recent years	207:218	recent years	207:218	In recent years, organic kale demand has increased at near-doubling rates in the USA due to its perceived nutritional value.
31316128	4	5	theme	essential	613:621	arg1	micronutrients					631:644	daily essential mineral micronutrients	607:644	daily essential mineral micronutrients	607:644	The objective of this study was to determine the effect of organic cover cropping systems on subsequent kale biomass production and nutrient composition (protein, mineral, and prebiotic carbohydrate concentrations) and to assess organic kale as a potential whole food source of daily essential mineral micronutrients and prebiotic carbohydrates.
31316128	6	6	theme	organic	1113:1119	arg1	kale					1121:1124	Fresh organic kale	1107:1124	Fresh organic kale	1107:1124	Fresh organic kale has low to moderate concentrations of protein (1.3-6.0 g/100 g).
31316128	9	7	theme	significant	1582:1592	arg1	interaction					1594:1604	a significant interaction	1580:1604	a significant interaction between kale variety and organic cover crop with respect to biomass and nutrient concentration	1580:1699	Results also demonstrated a significant interaction between kale variety and organic cover crop with respect to biomass and nutrient concentration.
31316128	7	8	theme	suitable	1256:1263	arg1	cultivars					1270:1278	the most suitable kale cultivars	1247:1278	the most suitable kale cultivars for organic production without considerable biomass and nutrient composition losses	1247:1362	Study results indicate that Starbor and Red Russian are the most suitable kale cultivars for organic production without considerable biomass and nutrient composition losses.
31316128	7	8	theme	suitable	1256:1263	arg1	Red					1231:1233	Red	1231:1233	Red	1231:1233	Study results indicate that Starbor and Red Russian are the most suitable kale cultivars for organic production without considerable biomass and nutrient composition losses.
31316128	7	8	theme	suitable	1256:1263	arg1	Starbor					1219:1225	Starbor	1219:1225	Starbor	1219:1225	Study results indicate that Starbor and Red Russian are the most suitable kale cultivars for organic production without considerable biomass and nutrient composition losses.
31316128	4	9	theme	potential	576:584	arg1	kale					566:569	organic kale	558:569	organic kale	558:569	The objective of this study was to determine the effect of organic cover cropping systems on subsequent kale biomass production and nutrient composition (protein, mineral, and prebiotic carbohydrate concentrations) and to assess organic kale as a potential whole food source of daily essential mineral micronutrients and prebiotic carbohydrates.
31316128	4	9	theme	potential	576:584	arg1	source					597:602	a potential whole food source	574:602	a potential whole food source of daily essential mineral micronutrients and prebiotic carbohydrates	574:672	The objective of this study was to determine the effect of organic cover cropping systems on subsequent kale biomass production and nutrient composition (protein, mineral, and prebiotic carbohydrate concentrations) and to assess organic kale as a potential whole food source of daily essential mineral micronutrients and prebiotic carbohydrates.
31316128	7	10	dep	Starbor	1219:1225	arg1	Russian					1235:1241	Russian	1235:1241	Russian	1235:1241	Study results indicate that Starbor and Red Russian are the most suitable kale cultivars for organic production without considerable biomass and nutrient composition losses.
31316128	4	11	theme	carbohydrates	660:672	arg1	kale					566:569	organic kale	558:569	organic kale	558:569	The objective of this study was to determine the effect of organic cover cropping systems on subsequent kale biomass production and nutrient composition (protein, mineral, and prebiotic carbohydrate concentrations) and to assess organic kale as a potential whole food source of daily essential mineral micronutrients and prebiotic carbohydrates.
31316128	4	11	theme	carbohydrates	660:672	arg1	source					597:602	a potential whole food source	574:602	a potential whole food source of daily essential mineral micronutrients and prebiotic carbohydrates	574:672	The objective of this study was to determine the effect of organic cover cropping systems on subsequent kale biomass production and nutrient composition (protein, mineral, and prebiotic carbohydrate concentrations) and to assess organic kale as a potential whole food source of daily essential mineral micronutrients and prebiotic carbohydrates.
31316128	3	12	theme	kale	229:232	arg1	demand					234:239	organic kale demand	221:239	organic kale demand	221:239	In recent years, organic kale demand has increased at near-doubling rates in the USA due to its perceived nutritional value.
31316128	4	13	theme	organic	388:394	arg1	systems					411:417	organic cover cropping systems	388:417	organic cover cropping systems	388:417	The objective of this study was to determine the effect of organic cover cropping systems on subsequent kale biomass production and nutrient composition (protein, mineral, and prebiotic carbohydrate concentrations) and to assess organic kale as a potential whole food source of daily essential mineral micronutrients and prebiotic carbohydrates.
31316128	6	14	theme	Fresh	1107:1111	arg1	kale					1121:1124	Fresh organic kale	1107:1124	Fresh organic kale	1107:1124	Fresh organic kale has low to moderate concentrations of protein (1.3-6.0 g/100 g).
31316128	4	15	theme	food	592:595	arg1	kale					566:569	organic kale	558:569	organic kale	558:569	The objective of this study was to determine the effect of organic cover cropping systems on subsequent kale biomass production and nutrient composition (protein, mineral, and prebiotic carbohydrate concentrations) and to assess organic kale as a potential whole food source of daily essential mineral micronutrients and prebiotic carbohydrates.
31316128	4	15	theme	food	592:595	arg1	source					597:602	a potential whole food source	574:602	a potential whole food source of daily essential mineral micronutrients and prebiotic carbohydrates	574:672	The objective of this study was to determine the effect of organic cover cropping systems on subsequent kale biomass production and nutrient composition (protein, mineral, and prebiotic carbohydrate concentrations) and to assess organic kale as a potential whole food source of daily essential mineral micronutrients and prebiotic carbohydrates.
31316128	5	16	theme	single	677:682	arg1	100-g					684:688	A single 100-g	675:688	A single 100-g serving of fresh organic kale	675:718	A single 100-g serving of fresh organic kale can provide mineral micronutrients (43-438 mg Ca; 11-60 mg Mg; 28-102 mg P; 0.5-3.3 mg Fe; 0.3-1.3 mg Mn; 1-136 µg Cu; and 0-35 µg Se) as well as 5.7-8.7 g of total prebiotic carbohydrates, including sugar alcohols (0.4-6.6 mg), simple sugars (6-1507 mg), raffinose and fructooligosaccharides (0.8-169 mg), hemicellulose (77-763 mg), lignin (0-90 mg), and unknown dietary fiber (5-6 g).
31316128	10	17	theme	organic	1709:1715	arg1	breeding					1729:1736	Future organic nutritional breeding	1702:1736	Future organic nutritional breeding of kale	1702:1744	Future organic nutritional breeding of kale is possible by selecting cultivars that perform well following different cover crops.
31316128	5	18	theme	0.5-3.3 mg	796:805	arg1	micronutrients					740:753	mineral micronutrients	732:753	mineral micronutrients (43-438 mg Ca; 11-60 mg Mg; 28-102 mg P; 0.5-3.3 mg Fe; 0.3-1.3 mg Mn; 1-136 µg Cu; and 0-35 µg Se)	732:853	A single 100-g serving of fresh organic kale can provide mineral micronutrients (43-438 mg Ca; 11-60 mg Mg; 28-102 mg P; 0.5-3.3 mg Fe; 0.3-1.3 mg Mn; 1-136 µg Cu; and 0-35 µg Se) as well as 5.7-8.7 g of total prebiotic carbohydrates, including sugar alcohols (0.4-6.6 mg), simple sugars (6-1507 mg), raffinose and fructooligosaccharides (0.8-169 mg), hemicellulose (77-763 mg), lignin (0-90 mg), and unknown dietary fiber (5-6 g).
31316128	5	18	theme	0.5-3.3 mg	796:805	arg1	Fe					807:808	0.5-3.3 mg Fe	796:808	0.5-3.3 mg Fe	796:808	A single 100-g serving of fresh organic kale can provide mineral micronutrients (43-438 mg Ca; 11-60 mg Mg; 28-102 mg P; 0.5-3.3 mg Fe; 0.3-1.3 mg Mn; 1-136 µg Cu; and 0-35 µg Se) as well as 5.7-8.7 g of total prebiotic carbohydrates, including sugar alcohols (0.4-6.6 mg), simple sugars (6-1507 mg), raffinose and fructooligosaccharides (0.8-169 mg), hemicellulose (77-763 mg), lignin (0-90 mg), and unknown dietary fiber (5-6 g).
31316128	4	19	theme	cropping	402:409	arg1	systems					411:417	organic cover cropping systems	388:417	organic cover cropping systems	388:417	The objective of this study was to determine the effect of organic cover cropping systems on subsequent kale biomass production and nutrient composition (protein, mineral, and prebiotic carbohydrate concentrations) and to assess organic kale as a potential whole food source of daily essential mineral micronutrients and prebiotic carbohydrates.
31316128	4	20	from	effect	378:383	arg1	production					446:455	subsequent kale biomass production	422:455	subsequent kale biomass production	422:455	The objective of this study was to determine the effect of organic cover cropping systems on subsequent kale biomass production and nutrient composition (protein, mineral, and prebiotic carbohydrate concentrations) and to assess organic kale as a potential whole food source of daily essential mineral micronutrients and prebiotic carbohydrates.
31316128	4	20	from	effect	378:383	arg1	composition					470:480	nutrient composition	461:480	nutrient composition (protein, mineral, and prebiotic carbohydrate concentrations)	461:542	The objective of this study was to determine the effect of organic cover cropping systems on subsequent kale biomass production and nutrient composition (protein, mineral, and prebiotic carbohydrate concentrations) and to assess organic kale as a potential whole food source of daily essential mineral micronutrients and prebiotic carbohydrates.
31316128	5	21	theme	simple	949:954	arg1	6-1507 mg					964:972	6-1507 mg	964:972	6-1507 mg	964:972	A single 100-g serving of fresh organic kale can provide mineral micronutrients (43-438 mg Ca; 11-60 mg Mg; 28-102 mg P; 0.5-3.3 mg Fe; 0.3-1.3 mg Mn; 1-136 µg Cu; and 0-35 µg Se) as well as 5.7-8.7 g of total prebiotic carbohydrates, including sugar alcohols (0.4-6.6 mg), simple sugars (6-1507 mg), raffinose and fructooligosaccharides (0.8-169 mg), hemicellulose (77-763 mg), lignin (0-90 mg), and unknown dietary fiber (5-6 g).
31316128	5	21	theme	simple	949:954	arg1	sugars					956:961	simple sugars	949:961	simple sugars (6-1507 mg)	949:973	A single 100-g serving of fresh organic kale can provide mineral micronutrients (43-438 mg Ca; 11-60 mg Mg; 28-102 mg P; 0.5-3.3 mg Fe; 0.3-1.3 mg Mn; 1-136 µg Cu; and 0-35 µg Se) as well as 5.7-8.7 g of total prebiotic carbohydrates, including sugar alcohols (0.4-6.6 mg), simple sugars (6-1507 mg), raffinose and fructooligosaccharides (0.8-169 mg), hemicellulose (77-763 mg), lignin (0-90 mg), and unknown dietary fiber (5-6 g).
31316128	2	22	theme	agricultural	182:193	arg1	systems					195:201	non-organic agricultural systems	170:201	non-organic agricultural systems	170:201	Kale is a leafy green vegetable regularly grown using non-organic agricultural systems.
31316128	5	23	theme	0-35 µg	843:849	arg1	Se					851:852	0-35 µg Se	843:852	0-35 µg Se	843:852	A single 100-g serving of fresh organic kale can provide mineral micronutrients (43-438 mg Ca; 11-60 mg Mg; 28-102 mg P; 0.5-3.3 mg Fe; 0.3-1.3 mg Mn; 1-136 µg Cu; and 0-35 µg Se) as well as 5.7-8.7 g of total prebiotic carbohydrates, including sugar alcohols (0.4-6.6 mg), simple sugars (6-1507 mg), raffinose and fructooligosaccharides (0.8-169 mg), hemicellulose (77-763 mg), lignin (0-90 mg), and unknown dietary fiber (5-6 g).
31316128	5	23	theme	0-35 µg	843:849	arg1	micronutrients					740:753	mineral micronutrients	732:753	mineral micronutrients (43-438 mg Ca; 11-60 mg Mg; 28-102 mg P; 0.5-3.3 mg Fe; 0.3-1.3 mg Mn; 1-136 µg Cu; and 0-35 µg Se)	732:853	A single 100-g serving of fresh organic kale can provide mineral micronutrients (43-438 mg Ca; 11-60 mg Mg; 28-102 mg P; 0.5-3.3 mg Fe; 0.3-1.3 mg Mn; 1-136 µg Cu; and 0-35 µg Se) as well as 5.7-8.7 g of total prebiotic carbohydrates, including sugar alcohols (0.4-6.6 mg), simple sugars (6-1507 mg), raffinose and fructooligosaccharides (0.8-169 mg), hemicellulose (77-763 mg), lignin (0-90 mg), and unknown dietary fiber (5-6 g).
31316128	9	24	theme	organic	1631:1637	arg1	crop					1645:1648	organic cover crop	1631:1648	organic cover crop	1631:1648	Results also demonstrated a significant interaction between kale variety and organic cover crop with respect to biomass and nutrient concentration.
31316128	6	25	theme	protein	1164:1170	arg1	concentrations					1146:1159	moderate concentrations	1137:1159	moderate concentrations of protein (1.3-6.0 g/100 g)	1137:1188	Fresh organic kale has low to moderate concentrations of protein (1.3-6.0 g/100 g).
31316128	10	26	theme	kale	1741:1744	arg1	breeding					1729:1736	Future organic nutritional breeding	1702:1736	Future organic nutritional breeding of kale	1702:1744	Future organic nutritional breeding of kale is possible by selecting cultivars that perform well following different cover crops.
31316128	3	27	theme	due	289:291	arg1	USA					285:287	the USA	281:287	the USA due to its perceived nutritional value	281:326	In recent years, organic kale demand has increased at near-doubling rates in the USA due to its perceived nutritional value.
31316128	4	28	theme	micronutrients	631:644	arg1	kale					566:569	organic kale	558:569	organic kale	558:569	The objective of this study was to determine the effect of organic cover cropping systems on subsequent kale biomass production and nutrient composition (protein, mineral, and prebiotic carbohydrate concentrations) and to assess organic kale as a potential whole food source of daily essential mineral micronutrients and prebiotic carbohydrates.
31316128	4	28	theme	micronutrients	631:644	arg1	source					597:602	a potential whole food source	574:602	a potential whole food source of daily essential mineral micronutrients and prebiotic carbohydrates	574:672	The objective of this study was to determine the effect of organic cover cropping systems on subsequent kale biomass production and nutrient composition (protein, mineral, and prebiotic carbohydrate concentrations) and to assess organic kale as a potential whole food source of daily essential mineral micronutrients and prebiotic carbohydrates.
31316128	0	29	theme	nutrient	39:46	arg1	concentration					48:60	nutrient concentration	39:60	nutrient concentration	39:60	Effect of cover crops on the yield and nutrient concentration of organic kale (Brassica oleracea L. var.
31316128	8	30	theme	cover	1375:1379	arg1	crops					1381:1385	the cover crops	1371:1385	the cover crops	1371:1385	Among the cover crops, faba bean results in the highest mineral, protein, and prebiotic carbohydrate concentrations in subsequent kale crops but ryegrass increases kale biomass production.
31316128	4	31	theme	prebiotic	650:658	arg1	carbohydrates					660:672	prebiotic carbohydrates	650:672	prebiotic carbohydrates	650:672	The objective of this study was to determine the effect of organic cover cropping systems on subsequent kale biomass production and nutrient composition (protein, mineral, and prebiotic carbohydrate concentrations) and to assess organic kale as a potential whole food source of daily essential mineral micronutrients and prebiotic carbohydrates.
31316128	3	32	theme	nutritional	310:320	arg1	value					322:326	its perceived nutritional value	296:326	its perceived nutritional value	296:326	In recent years, organic kale demand has increased at near-doubling rates in the USA due to its perceived nutritional value.
31316128	4	33	theme	nutrient	461:468	arg1	composition					470:480	nutrient composition	461:480	nutrient composition (protein, mineral, and prebiotic carbohydrate concentrations)	461:542	The objective of this study was to determine the effect of organic cover cropping systems on subsequent kale biomass production and nutrient composition (protein, mineral, and prebiotic carbohydrate concentrations) and to assess organic kale as a potential whole food source of daily essential mineral micronutrients and prebiotic carbohydrates.
31316128	0	34	theme	kale	73:76	arg1	yield					29:33	yield	29:33	yield	29:33	Effect of cover crops on the yield and nutrient concentration of organic kale (Brassica oleracea L. var.
31316128	0	34	theme	kale	73:76	arg1	concentration					48:60	nutrient concentration	39:60	nutrient concentration	39:60	Effect of cover crops on the yield and nutrient concentration of organic kale (Brassica oleracea L. var.
31316128	5	35	theme	total	879:883	arg1	carbohydrates					895:907	total prebiotic carbohydrates	879:907	total prebiotic carbohydrates	879:907	A single 100-g serving of fresh organic kale can provide mineral micronutrients (43-438 mg Ca; 11-60 mg Mg; 28-102 mg P; 0.5-3.3 mg Fe; 0.3-1.3 mg Mn; 1-136 µg Cu; and 0-35 µg Se) as well as 5.7-8.7 g of total prebiotic carbohydrates, including sugar alcohols (0.4-6.6 mg), simple sugars (6-1507 mg), raffinose and fructooligosaccharides (0.8-169 mg), hemicellulose (77-763 mg), lignin (0-90 mg), and unknown dietary fiber (5-6 g).
31316128	9	36	theme	nutrient	1678:1685	arg1	concentration					1687:1699	biomass and nutrient concentration	1666:1699	concentration	1687:1699	Results also demonstrated a significant interaction between kale variety and organic cover crop with respect to biomass and nutrient concentration.
31316128	5	37	theme	fresh	701:705	arg1	kale					715:718	fresh organic kale	701:718	fresh organic kale	701:718	A single 100-g serving of fresh organic kale can provide mineral micronutrients (43-438 mg Ca; 11-60 mg Mg; 28-102 mg P; 0.5-3.3 mg Fe; 0.3-1.3 mg Mn; 1-136 µg Cu; and 0-35 µg Se) as well as 5.7-8.7 g of total prebiotic carbohydrates, including sugar alcohols (0.4-6.6 mg), simple sugars (6-1507 mg), raffinose and fructooligosaccharides (0.8-169 mg), hemicellulose (77-763 mg), lignin (0-90 mg), and unknown dietary fiber (5-6 g).
31316128	5	38	theme	carbohydrates	895:907	arg1	5.7-8.7 g					866:874	5.7-8.7 g	866:874	5.7-8.7 g	866:874	A single 100-g serving of fresh organic kale can provide mineral micronutrients (43-438 mg Ca; 11-60 mg Mg; 28-102 mg P; 0.5-3.3 mg Fe; 0.3-1.3 mg Mn; 1-136 µg Cu; and 0-35 µg Se) as well as 5.7-8.7 g of total prebiotic carbohydrates, including sugar alcohols (0.4-6.6 mg), simple sugars (6-1507 mg), raffinose and fructooligosaccharides (0.8-169 mg), hemicellulose (77-763 mg), lignin (0-90 mg), and unknown dietary fiber (5-6 g).
31316128	4	39	theme	kale	433:436	arg1	production					446:455	subsequent kale biomass production	422:455	subsequent kale biomass production	422:455	The objective of this study was to determine the effect of organic cover cropping systems on subsequent kale biomass production and nutrient composition (protein, mineral, and prebiotic carbohydrate concentrations) and to assess organic kale as a potential whole food source of daily essential mineral micronutrients and prebiotic carbohydrates.
31316128	7	40	theme	Study	1191:1195	arg1	results					1197:1203	Study results	1191:1203	Study results	1191:1203	Study results indicate that Starbor and Red Russian are the most suitable kale cultivars for organic production without considerable biomass and nutrient composition losses.
31316128	8	41	theme	highest	1413:1419	arg1	protein					1430:1436	the highest mineral, protein, and prebiotic carbohydrate concentrations	1409:1479	protein	1430:1436	Among the cover crops, faba bean results in the highest mineral, protein, and prebiotic carbohydrate concentrations in subsequent kale crops but ryegrass increases kale biomass production.
31316128	5	42	theme	5.7-8.7 g	866:874	arg1	hemicellulose					1027:1039	well as 5.7-8.7 g of total prebiotic carbohydrates, including sugar alcohols (0.4-6.6 mg), simple sugars (6-1507 mg), raffinose and fructooligosaccharides (0.8-169 mg), hemicellulose	858:1039	well as 5.7-8.7 g of total prebiotic carbohydrates, including sugar alcohols (0.4-6.6 mg), simple sugars (6-1507 mg), raffinose and fructooligosaccharides (0.8-169 mg), hemicellulose (77-763 mg)	858:1051	A single 100-g serving of fresh organic kale can provide mineral micronutrients (43-438 mg Ca; 11-60 mg Mg; 28-102 mg P; 0.5-3.3 mg Fe; 0.3-1.3 mg Mn; 1-136 µg Cu; and 0-35 µg Se) as well as 5.7-8.7 g of total prebiotic carbohydrates, including sugar alcohols (0.4-6.6 mg), simple sugars (6-1507 mg), raffinose and fructooligosaccharides (0.8-169 mg), hemicellulose (77-763 mg), lignin (0-90 mg), and unknown dietary fiber (5-6 g).
31316128	5	42	theme	5.7-8.7 g	866:874	arg1	5-6 g					1099:1103	5-6 g	1099:1103	5-6 g	1099:1103	A single 100-g serving of fresh organic kale can provide mineral micronutrients (43-438 mg Ca; 11-60 mg Mg; 28-102 mg P; 0.5-3.3 mg Fe; 0.3-1.3 mg Mn; 1-136 µg Cu; and 0-35 µg Se) as well as 5.7-8.7 g of total prebiotic carbohydrates, including sugar alcohols (0.4-6.6 mg), simple sugars (6-1507 mg), raffinose and fructooligosaccharides (0.8-169 mg), hemicellulose (77-763 mg), lignin (0-90 mg), and unknown dietary fiber (5-6 g).
31316128	5	42	theme	5.7-8.7 g	866:874	arg1	77-763 mg					1042:1050	77-763 mg	1042:1050	77-763 mg	1042:1050	A single 100-g serving of fresh organic kale can provide mineral micronutrients (43-438 mg Ca; 11-60 mg Mg; 28-102 mg P; 0.5-3.3 mg Fe; 0.3-1.3 mg Mn; 1-136 µg Cu; and 0-35 µg Se) as well as 5.7-8.7 g of total prebiotic carbohydrates, including sugar alcohols (0.4-6.6 mg), simple sugars (6-1507 mg), raffinose and fructooligosaccharides (0.8-169 mg), hemicellulose (77-763 mg), lignin (0-90 mg), and unknown dietary fiber (5-6 g).
31316128	4	43	theme	prebiotic	505:513	arg1	concentrations					528:541	prebiotic carbohydrate concentrations	505:541	prebiotic carbohydrate concentrations	505:541	The objective of this study was to determine the effect of organic cover cropping systems on subsequent kale biomass production and nutrient composition (protein, mineral, and prebiotic carbohydrate concentrations) and to assess organic kale as a potential whole food source of daily essential mineral micronutrients and prebiotic carbohydrates.
31316128	2	44	theme	green	132:136	arg1	vegetable					138:146	a leafy green vegetable	124:146	a leafy green vegetable regularly grown using non-organic agricultural systems	124:201	Kale is a leafy green vegetable regularly grown using non-organic agricultural systems.
31316128	2	44	theme	green	132:136	arg1	Kale					116:119	Kale	116:119	Kale	116:119	Kale is a leafy green vegetable regularly grown using non-organic agricultural systems.
31316128	4	45	dep	composition	470:480	arg1	concentrations					528:541	prebiotic carbohydrate concentrations	505:541	prebiotic carbohydrate concentrations	505:541	The objective of this study was to determine the effect of organic cover cropping systems on subsequent kale biomass production and nutrient composition (protein, mineral, and prebiotic carbohydrate concentrations) and to assess organic kale as a potential whole food source of daily essential mineral micronutrients and prebiotic carbohydrates.
31316128	4	45	dep	composition	470:480	arg1	mineral					492:498	mineral	492:498	mineral	492:498	The objective of this study was to determine the effect of organic cover cropping systems on subsequent kale biomass production and nutrient composition (protein, mineral, and prebiotic carbohydrate concentrations) and to assess organic kale as a potential whole food source of daily essential mineral micronutrients and prebiotic carbohydrates.
31316128	4	45	dep	composition	470:480	arg1	protein					483:489	protein	483:489	protein	483:489	The objective of this study was to determine the effect of organic cover cropping systems on subsequent kale biomass production and nutrient composition (protein, mineral, and prebiotic carbohydrate concentrations) and to assess organic kale as a potential whole food source of daily essential mineral micronutrients and prebiotic carbohydrates.
31316128	0	46	dep	yield	29:33	arg1	the					25:27	the	25:27	the	25:27	Effect of cover crops on the yield and nutrient concentration of organic kale (Brassica oleracea L. var.
31316128	10	47	dep	different	1809:1817	arg1	cover					1819:1823	cover	1819:1823	cover crops	1819:1829	Future organic nutritional breeding of kale is possible by selecting cultivars that perform well following different cover crops.
31316128	5	48	theme	43-438 mg	756:764	arg1	micronutrients					740:753	mineral micronutrients	732:753	mineral micronutrients (43-438 mg Ca; 11-60 mg Mg; 28-102 mg P; 0.5-3.3 mg Fe; 0.3-1.3 mg Mn; 1-136 µg Cu; and 0-35 µg Se)	732:853	A single 100-g serving of fresh organic kale can provide mineral micronutrients (43-438 mg Ca; 11-60 mg Mg; 28-102 mg P; 0.5-3.3 mg Fe; 0.3-1.3 mg Mn; 1-136 µg Cu; and 0-35 µg Se) as well as 5.7-8.7 g of total prebiotic carbohydrates, including sugar alcohols (0.4-6.6 mg), simple sugars (6-1507 mg), raffinose and fructooligosaccharides (0.8-169 mg), hemicellulose (77-763 mg), lignin (0-90 mg), and unknown dietary fiber (5-6 g).
31316128	5	48	theme	43-438 mg	756:764	arg1	Ca					766:767	43-438 mg Ca	756:767	43-438 mg Ca	756:767	A single 100-g serving of fresh organic kale can provide mineral micronutrients (43-438 mg Ca; 11-60 mg Mg; 28-102 mg P; 0.5-3.3 mg Fe; 0.3-1.3 mg Mn; 1-136 µg Cu; and 0-35 µg Se) as well as 5.7-8.7 g of total prebiotic carbohydrates, including sugar alcohols (0.4-6.6 mg), simple sugars (6-1507 mg), raffinose and fructooligosaccharides (0.8-169 mg), hemicellulose (77-763 mg), lignin (0-90 mg), and unknown dietary fiber (5-6 g).
31316128	8	49	theme	prebiotic	1443:1451	arg1	concentrations					1466:1479	the highest mineral, protein, and prebiotic carbohydrate concentrations	1409:1479	concentrations	1466:1479	Among the cover crops, faba bean results in the highest mineral, protein, and prebiotic carbohydrate concentrations in subsequent kale crops but ryegrass increases kale biomass production.
31316128	8	50	theme	kale	1529:1532	arg1	biomass					1534:1540	kale biomass	1529:1540	kale biomass production	1529:1551	Among the cover crops, faba bean results in the highest mineral, protein, and prebiotic carbohydrate concentrations in subsequent kale crops but ryegrass increases kale biomass production.
31316128	0	51	dep	cover	10:14	arg1	Brassica					79:86	Brassica	79:86	Brassica	79:86	Effect of cover crops on the yield and nutrient concentration of organic kale (Brassica oleracea L. var.
31316128	8	52	theme	subsequent	1484:1493	arg1	crops					1500:1504	subsequent kale crops	1484:1504	subsequent kale crops	1484:1504	Among the cover crops, faba bean results in the highest mineral, protein, and prebiotic carbohydrate concentrations in subsequent kale crops but ryegrass increases kale biomass production.
31316128	8	53	from	protein	1430:1436	arg1	crops					1500:1504	subsequent kale crops	1484:1504	subsequent kale crops	1484:1504	Among the cover crops, faba bean results in the highest mineral, protein, and prebiotic carbohydrate concentrations in subsequent kale crops but ryegrass increases kale biomass production.
31316128	4	54	dep	systems	411:417	arg1	cover					396:400	cover	396:400	cover	396:400	The objective of this study was to determine the effect of organic cover cropping systems on subsequent kale biomass production and nutrient composition (protein, mineral, and prebiotic carbohydrate concentrations) and to assess organic kale as a potential whole food source of daily essential mineral micronutrients and prebiotic carbohydrates.
31316128	4	55	theme	study	351:355	arg1	objective					333:341	The objective	329:341	The objective of this study	329:355	The objective of this study was to determine the effect of organic cover cropping systems on subsequent kale biomass production and nutrient composition (protein, mineral, and prebiotic carbohydrate concentrations) and to assess organic kale as a potential whole food source of daily essential mineral micronutrients and prebiotic carbohydrates.
31316128	6	56	theme	moderate	1137:1144	arg1	concentrations					1146:1159	moderate concentrations	1137:1159	moderate concentrations of protein (1.3-6.0 g/100 g)	1137:1188	Fresh organic kale has low to moderate concentrations of protein (1.3-6.0 g/100 g).
31316128	7	57	theme	nutrient	1336:1343	arg1	composition					1345:1355	nutrient composition	1336:1355	nutrient composition	1336:1355	Study results indicate that Starbor and Red Russian are the most suitable kale cultivars for organic production without considerable biomass and nutrient composition losses.
31316128	7	58	theme	considerable	1311:1322	arg1	biomass					1324:1330	considerable biomass	1311:1330	considerable biomass	1311:1330	Study results indicate that Starbor and Red Russian are the most suitable kale cultivars for organic production without considerable biomass and nutrient composition losses.
31316128	7	59	theme	biomass	1324:1330	arg1	losses					1357:1362	considerable biomass and nutrient composition losses	1311:1362	considerable biomass and nutrient composition losses	1311:1362	Study results indicate that Starbor and Red Russian are the most suitable kale cultivars for organic production without considerable biomass and nutrient composition losses.
31316128	3	60	theme	organic	221:227	arg1	demand					234:239	organic kale demand	221:239	organic kale demand	221:239	In recent years, organic kale demand has increased at near-doubling rates in the USA due to its perceived nutritional value.
31316128	5	61	theme	0.3-1.3 mg	811:820	arg1	micronutrients					740:753	mineral micronutrients	732:753	mineral micronutrients (43-438 mg Ca; 11-60 mg Mg; 28-102 mg P; 0.5-3.3 mg Fe; 0.3-1.3 mg Mn; 1-136 µg Cu; and 0-35 µg Se)	732:853	A single 100-g serving of fresh organic kale can provide mineral micronutrients (43-438 mg Ca; 11-60 mg Mg; 28-102 mg P; 0.5-3.3 mg Fe; 0.3-1.3 mg Mn; 1-136 µg Cu; and 0-35 µg Se) as well as 5.7-8.7 g of total prebiotic carbohydrates, including sugar alcohols (0.4-6.6 mg), simple sugars (6-1507 mg), raffinose and fructooligosaccharides (0.8-169 mg), hemicellulose (77-763 mg), lignin (0-90 mg), and unknown dietary fiber (5-6 g).
31316128	5	61	theme	0.3-1.3 mg	811:820	arg1	Mn					822:823	0.3-1.3 mg Mn	811:823	0.3-1.3 mg Mn	811:823	A single 100-g serving of fresh organic kale can provide mineral micronutrients (43-438 mg Ca; 11-60 mg Mg; 28-102 mg P; 0.5-3.3 mg Fe; 0.3-1.3 mg Mn; 1-136 µg Cu; and 0-35 µg Se) as well as 5.7-8.7 g of total prebiotic carbohydrates, including sugar alcohols (0.4-6.6 mg), simple sugars (6-1507 mg), raffinose and fructooligosaccharides (0.8-169 mg), hemicellulose (77-763 mg), lignin (0-90 mg), and unknown dietary fiber (5-6 g).
31316128	5	62	dep	micronutrients	740:753	arg1	Se					851:852	0-35 µg Se	843:852	0-35 µg Se	843:852	A single 100-g serving of fresh organic kale can provide mineral micronutrients (43-438 mg Ca; 11-60 mg Mg; 28-102 mg P; 0.5-3.3 mg Fe; 0.3-1.3 mg Mn; 1-136 µg Cu; and 0-35 µg Se) as well as 5.7-8.7 g of total prebiotic carbohydrates, including sugar alcohols (0.4-6.6 mg), simple sugars (6-1507 mg), raffinose and fructooligosaccharides (0.8-169 mg), hemicellulose (77-763 mg), lignin (0-90 mg), and unknown dietary fiber (5-6 g).
31316128	5	62	dep	micronutrients	740:753	arg1	28-102 mg P					783:793	28-102 mg P	783:793	28-102 mg P	783:793	A single 100-g serving of fresh organic kale can provide mineral micronutrients (43-438 mg Ca; 11-60 mg Mg; 28-102 mg P; 0.5-3.3 mg Fe; 0.3-1.3 mg Mn; 1-136 µg Cu; and 0-35 µg Se) as well as 5.7-8.7 g of total prebiotic carbohydrates, including sugar alcohols (0.4-6.6 mg), simple sugars (6-1507 mg), raffinose and fructooligosaccharides (0.8-169 mg), hemicellulose (77-763 mg), lignin (0-90 mg), and unknown dietary fiber (5-6 g).
31316128	5	62	dep	micronutrients	740:753	arg1	Ca					766:767	43-438 mg Ca	756:767	43-438 mg Ca	756:767	A single 100-g serving of fresh organic kale can provide mineral micronutrients (43-438 mg Ca; 11-60 mg Mg; 28-102 mg P; 0.5-3.3 mg Fe; 0.3-1.3 mg Mn; 1-136 µg Cu; and 0-35 µg Se) as well as 5.7-8.7 g of total prebiotic carbohydrates, including sugar alcohols (0.4-6.6 mg), simple sugars (6-1507 mg), raffinose and fructooligosaccharides (0.8-169 mg), hemicellulose (77-763 mg), lignin (0-90 mg), and unknown dietary fiber (5-6 g).
31316128	5	62	dep	micronutrients	740:753	arg1	Cu					835:836	1-136 µg Cu	826:836	1-136 µg Cu	826:836	A single 100-g serving of fresh organic kale can provide mineral micronutrients (43-438 mg Ca; 11-60 mg Mg; 28-102 mg P; 0.5-3.3 mg Fe; 0.3-1.3 mg Mn; 1-136 µg Cu; and 0-35 µg Se) as well as 5.7-8.7 g of total prebiotic carbohydrates, including sugar alcohols (0.4-6.6 mg), simple sugars (6-1507 mg), raffinose and fructooligosaccharides (0.8-169 mg), hemicellulose (77-763 mg), lignin (0-90 mg), and unknown dietary fiber (5-6 g).
31316128	5	62	dep	micronutrients	740:753	arg1	Fe					807:808	0.5-3.3 mg Fe	796:808	0.5-3.3 mg Fe	796:808	A single 100-g serving of fresh organic kale can provide mineral micronutrients (43-438 mg Ca; 11-60 mg Mg; 28-102 mg P; 0.5-3.3 mg Fe; 0.3-1.3 mg Mn; 1-136 µg Cu; and 0-35 µg Se) as well as 5.7-8.7 g of total prebiotic carbohydrates, including sugar alcohols (0.4-6.6 mg), simple sugars (6-1507 mg), raffinose and fructooligosaccharides (0.8-169 mg), hemicellulose (77-763 mg), lignin (0-90 mg), and unknown dietary fiber (5-6 g).
31316128	5	62	dep	micronutrients	740:753	arg1	Mg					779:780	11-60 mg Mg	770:780	11-60 mg Mg	770:780	A single 100-g serving of fresh organic kale can provide mineral micronutrients (43-438 mg Ca; 11-60 mg Mg; 28-102 mg P; 0.5-3.3 mg Fe; 0.3-1.3 mg Mn; 1-136 µg Cu; and 0-35 µg Se) as well as 5.7-8.7 g of total prebiotic carbohydrates, including sugar alcohols (0.4-6.6 mg), simple sugars (6-1507 mg), raffinose and fructooligosaccharides (0.8-169 mg), hemicellulose (77-763 mg), lignin (0-90 mg), and unknown dietary fiber (5-6 g).
31316128	5	62	dep	micronutrients	740:753	arg1	micronutrients					740:753	mineral micronutrients	732:753	mineral micronutrients (43-438 mg Ca; 11-60 mg Mg; 28-102 mg P; 0.5-3.3 mg Fe; 0.3-1.3 mg Mn; 1-136 µg Cu; and 0-35 µg Se)	732:853	A single 100-g serving of fresh organic kale can provide mineral micronutrients (43-438 mg Ca; 11-60 mg Mg; 28-102 mg P; 0.5-3.3 mg Fe; 0.3-1.3 mg Mn; 1-136 µg Cu; and 0-35 µg Se) as well as 5.7-8.7 g of total prebiotic carbohydrates, including sugar alcohols (0.4-6.6 mg), simple sugars (6-1507 mg), raffinose and fructooligosaccharides (0.8-169 mg), hemicellulose (77-763 mg), lignin (0-90 mg), and unknown dietary fiber (5-6 g).
31316128	5	62	dep	micronutrients	740:753	arg1	Mn					822:823	0.3-1.3 mg Mn	811:823	0.3-1.3 mg Mn	811:823	A single 100-g serving of fresh organic kale can provide mineral micronutrients (43-438 mg Ca; 11-60 mg Mg; 28-102 mg P; 0.5-3.3 mg Fe; 0.3-1.3 mg Mn; 1-136 µg Cu; and 0-35 µg Se) as well as 5.7-8.7 g of total prebiotic carbohydrates, including sugar alcohols (0.4-6.6 mg), simple sugars (6-1507 mg), raffinose and fructooligosaccharides (0.8-169 mg), hemicellulose (77-763 mg), lignin (0-90 mg), and unknown dietary fiber (5-6 g).
31316128	4	63	theme	daily	607:611	arg1	micronutrients					631:644	daily essential mineral micronutrients	607:644	daily essential mineral micronutrients	607:644	The objective of this study was to determine the effect of organic cover cropping systems on subsequent kale biomass production and nutrient composition (protein, mineral, and prebiotic carbohydrate concentrations) and to assess organic kale as a potential whole food source of daily essential mineral micronutrients and prebiotic carbohydrates.
31316128	7	64	theme	kale	1265:1268	arg1	cultivars					1270:1278	the most suitable kale cultivars	1247:1278	the most suitable kale cultivars for organic production without considerable biomass and nutrient composition losses	1247:1362	Study results indicate that Starbor and Red Russian are the most suitable kale cultivars for organic production without considerable biomass and nutrient composition losses.
31316128	7	64	theme	kale	1265:1268	arg1	Red					1231:1233	Red	1231:1233	Red	1231:1233	Study results indicate that Starbor and Red Russian are the most suitable kale cultivars for organic production without considerable biomass and nutrient composition losses.
31316128	7	64	theme	kale	1265:1268	arg1	Starbor					1219:1225	Starbor	1219:1225	Starbor	1219:1225	Study results indicate that Starbor and Red Russian are the most suitable kale cultivars for organic production without considerable biomass and nutrient composition losses.
31316128	9	65	dep	crop	1645:1648	arg1	cover					1639:1643	cover	1639:1643	cover	1639:1643	Results also demonstrated a significant interaction between kale variety and organic cover crop with respect to biomass and nutrient concentration.
31316128	10	66	theme	nutritional	1717:1727	arg1	breeding					1729:1736	Future organic nutritional breeding	1702:1736	Future organic nutritional breeding of kale	1702:1744	Future organic nutritional breeding of kale is possible by selecting cultivars that perform well following different cover crops.
31316128	9	67	theme	kale	1614:1617	arg1	variety					1619:1625	kale variety	1614:1625	kale variety	1614:1625	Results also demonstrated a significant interaction between kale variety and organic cover crop with respect to biomass and nutrient concentration.
31316128	4	68	theme	systems	411:417	arg1	effect					378:383	the effect	374:383	the effect of organic cover cropping systems on subsequent kale biomass production and nutrient composition (protein, mineral, and prebiotic carbohydrate concentrations)	374:542	The objective of this study was to determine the effect of organic cover cropping systems on subsequent kale biomass production and nutrient composition (protein, mineral, and prebiotic carbohydrate concentrations) and to assess organic kale as a potential whole food source of daily essential mineral micronutrients and prebiotic carbohydrates.
31316128	4	69	theme	whole	586:590	arg1	kale					566:569	organic kale	558:569	organic kale	558:569	The objective of this study was to determine the effect of organic cover cropping systems on subsequent kale biomass production and nutrient composition (protein, mineral, and prebiotic carbohydrate concentrations) and to assess organic kale as a potential whole food source of daily essential mineral micronutrients and prebiotic carbohydrates.
31316128	4	69	theme	whole	586:590	arg1	source					597:602	a potential whole food source	574:602	a potential whole food source of daily essential mineral micronutrients and prebiotic carbohydrates	574:672	The objective of this study was to determine the effect of organic cover cropping systems on subsequent kale biomass production and nutrient composition (protein, mineral, and prebiotic carbohydrate concentrations) and to assess organic kale as a potential whole food source of daily essential mineral micronutrients and prebiotic carbohydrates.
31316128	9	70	theme	biomass	1666:1672	arg1	concentration					1687:1699	biomass and nutrient concentration	1666:1699	concentration	1687:1699	Results also demonstrated a significant interaction between kale variety and organic cover crop with respect to biomass and nutrient concentration.
31316128	10	71	theme	Future	1702:1707	arg1	breeding					1729:1736	Future organic nutritional breeding	1702:1736	Future organic nutritional breeding of kale	1702:1744	Future organic nutritional breeding of kale is possible by selecting cultivars that perform well following different cover crops.
31316128	5	72	dep	carbohydrates	895:907	arg1	including					910:918	including	910:918	including sugar alcohols (0.4-6.6 mg), simple sugars (6-1507 mg), raffinose and fructooligosaccharides (0.8-169 mg)	910:1024	A single 100-g serving of fresh organic kale can provide mineral micronutrients (43-438 mg Ca; 11-60 mg Mg; 28-102 mg P; 0.5-3.3 mg Fe; 0.3-1.3 mg Mn; 1-136 µg Cu; and 0-35 µg Se) as well as 5.7-8.7 g of total prebiotic carbohydrates, including sugar alcohols (0.4-6.6 mg), simple sugars (6-1507 mg), raffinose and fructooligosaccharides (0.8-169 mg), hemicellulose (77-763 mg), lignin (0-90 mg), and unknown dietary fiber (5-6 g).
31316128	3	73	theme	near-doubling	258:270	arg1	rates					272:276	near-doubling rates	258:276	near-doubling rates	258:276	In recent years, organic kale demand has increased at near-doubling rates in the USA due to its perceived nutritional value.
31316128	5	74	theme	organic	707:713	arg1	kale					715:718	fresh organic kale	701:718	fresh organic kale	701:718	A single 100-g serving of fresh organic kale can provide mineral micronutrients (43-438 mg Ca; 11-60 mg Mg; 28-102 mg P; 0.5-3.3 mg Fe; 0.3-1.3 mg Mn; 1-136 µg Cu; and 0-35 µg Se) as well as 5.7-8.7 g of total prebiotic carbohydrates, including sugar alcohols (0.4-6.6 mg), simple sugars (6-1507 mg), raffinose and fructooligosaccharides (0.8-169 mg), hemicellulose (77-763 mg), lignin (0-90 mg), and unknown dietary fiber (5-6 g).
31316128	4	75	theme	mineral	623:629	arg1	micronutrients					631:644	daily essential mineral micronutrients	607:644	daily essential mineral micronutrients	607:644	The objective of this study was to determine the effect of organic cover cropping systems on subsequent kale biomass production and nutrient composition (protein, mineral, and prebiotic carbohydrate concentrations) and to assess organic kale as a potential whole food source of daily essential mineral micronutrients and prebiotic carbohydrates.
31316128	0	76	theme	organic	65:71	arg1	kale					73:76	organic kale	65:76	organic kale	65:76	Effect of cover crops on the yield and nutrient concentration of organic kale (Brassica oleracea L. var.
31316128	8	77	theme	faba	1388:1391	arg1	bean					1393:1396	faba bean	1388:1396	faba bean	1388:1396	Among the cover crops, faba bean results in the highest mineral, protein, and prebiotic carbohydrate concentrations in subsequent kale crops but ryegrass increases kale biomass production.
31316128	4	78	theme	subsequent	422:431	arg1	production					446:455	subsequent kale biomass production	422:455	subsequent kale biomass production	422:455	The objective of this study was to determine the effect of organic cover cropping systems on subsequent kale biomass production and nutrient composition (protein, mineral, and prebiotic carbohydrate concentrations) and to assess organic kale as a potential whole food source of daily essential mineral micronutrients and prebiotic carbohydrates.
31316128	0	79	dep	Brassica	79:86	arg1	L.					97:98	Brassica oleracea L.	79:98	Brassica oleracea L. var	79:102	Effect of cover crops on the yield and nutrient concentration of organic kale (Brassica oleracea L. var.
31316128	0	79	dep	Brassica	79:86	arg1	var					100:102	var	100:102	Brassica oleracea L. var	79:102	Effect of cover crops on the yield and nutrient concentration of organic kale (Brassica oleracea L. var.
31316128	5	80	theme	prebiotic	885:893	arg1	carbohydrates					895:907	total prebiotic carbohydrates	879:907	total prebiotic carbohydrates	879:907	A single 100-g serving of fresh organic kale can provide mineral micronutrients (43-438 mg Ca; 11-60 mg Mg; 28-102 mg P; 0.5-3.3 mg Fe; 0.3-1.3 mg Mn; 1-136 µg Cu; and 0-35 µg Se) as well as 5.7-8.7 g of total prebiotic carbohydrates, including sugar alcohols (0.4-6.6 mg), simple sugars (6-1507 mg), raffinose and fructooligosaccharides (0.8-169 mg), hemicellulose (77-763 mg), lignin (0-90 mg), and unknown dietary fiber (5-6 g).
31316128	3	81	theme	perceived	300:308	arg1	value					322:326	its perceived nutritional value	296:326	its perceived nutritional value	296:326	In recent years, organic kale demand has increased at near-doubling rates in the USA due to its perceived nutritional value.
31316128	4	82	theme	biomass	438:444	arg1	production					446:455	subsequent kale biomass production	422:455	subsequent kale biomass production	422:455	The objective of this study was to determine the effect of organic cover cropping systems on subsequent kale biomass production and nutrient composition (protein, mineral, and prebiotic carbohydrate concentrations) and to assess organic kale as a potential whole food source of daily essential mineral micronutrients and prebiotic carbohydrates.
31316128	8	83	theme	mineral	1421:1427	arg1	protein					1430:1436	the highest mineral, protein, and prebiotic carbohydrate concentrations	1409:1479	protein	1430:1436	Among the cover crops, faba bean results in the highest mineral, protein, and prebiotic carbohydrate concentrations in subsequent kale crops but ryegrass increases kale biomass production.
31316128	5	84	theme	11-60 mg	770:777	arg1	micronutrients					740:753	mineral micronutrients	732:753	mineral micronutrients (43-438 mg Ca; 11-60 mg Mg; 28-102 mg P; 0.5-3.3 mg Fe; 0.3-1.3 mg Mn; 1-136 µg Cu; and 0-35 µg Se)	732:853	A single 100-g serving of fresh organic kale can provide mineral micronutrients (43-438 mg Ca; 11-60 mg Mg; 28-102 mg P; 0.5-3.3 mg Fe; 0.3-1.3 mg Mn; 1-136 µg Cu; and 0-35 µg Se) as well as 5.7-8.7 g of total prebiotic carbohydrates, including sugar alcohols (0.4-6.6 mg), simple sugars (6-1507 mg), raffinose and fructooligosaccharides (0.8-169 mg), hemicellulose (77-763 mg), lignin (0-90 mg), and unknown dietary fiber (5-6 g).
31316128	5	84	theme	11-60 mg	770:777	arg1	Mg					779:780	11-60 mg Mg	770:780	11-60 mg Mg	770:780	A single 100-g serving of fresh organic kale can provide mineral micronutrients (43-438 mg Ca; 11-60 mg Mg; 28-102 mg P; 0.5-3.3 mg Fe; 0.3-1.3 mg Mn; 1-136 µg Cu; and 0-35 µg Se) as well as 5.7-8.7 g of total prebiotic carbohydrates, including sugar alcohols (0.4-6.6 mg), simple sugars (6-1507 mg), raffinose and fructooligosaccharides (0.8-169 mg), hemicellulose (77-763 mg), lignin (0-90 mg), and unknown dietary fiber (5-6 g).
31316128	4	85	theme	carbohydrate	515:526	arg1	concentrations					528:541	prebiotic carbohydrate concentrations	505:541	prebiotic carbohydrate concentrations	505:541	The objective of this study was to determine the effect of organic cover cropping systems on subsequent kale biomass production and nutrient composition (protein, mineral, and prebiotic carbohydrate concentrations) and to assess organic kale as a potential whole food source of daily essential mineral micronutrients and prebiotic carbohydrates.
31316128	5	86	theme	mineral	732:738	arg1	Se					851:852	0-35 µg Se	843:852	0-35 µg Se	843:852	A single 100-g serving of fresh organic kale can provide mineral micronutrients (43-438 mg Ca; 11-60 mg Mg; 28-102 mg P; 0.5-3.3 mg Fe; 0.3-1.3 mg Mn; 1-136 µg Cu; and 0-35 µg Se) as well as 5.7-8.7 g of total prebiotic carbohydrates, including sugar alcohols (0.4-6.6 mg), simple sugars (6-1507 mg), raffinose and fructooligosaccharides (0.8-169 mg), hemicellulose (77-763 mg), lignin (0-90 mg), and unknown dietary fiber (5-6 g).
31316128	5	86	theme	mineral	732:738	arg1	28-102 mg P					783:793	28-102 mg P	783:793	28-102 mg P	783:793	A single 100-g serving of fresh organic kale can provide mineral micronutrients (43-438 mg Ca; 11-60 mg Mg; 28-102 mg P; 0.5-3.3 mg Fe; 0.3-1.3 mg Mn; 1-136 µg Cu; and 0-35 µg Se) as well as 5.7-8.7 g of total prebiotic carbohydrates, including sugar alcohols (0.4-6.6 mg), simple sugars (6-1507 mg), raffinose and fructooligosaccharides (0.8-169 mg), hemicellulose (77-763 mg), lignin (0-90 mg), and unknown dietary fiber (5-6 g).
31316128	5	86	theme	mineral	732:738	arg1	Ca					766:767	43-438 mg Ca	756:767	43-438 mg Ca	756:767	A single 100-g serving of fresh organic kale can provide mineral micronutrients (43-438 mg Ca; 11-60 mg Mg; 28-102 mg P; 0.5-3.3 mg Fe; 0.3-1.3 mg Mn; 1-136 µg Cu; and 0-35 µg Se) as well as 5.7-8.7 g of total prebiotic carbohydrates, including sugar alcohols (0.4-6.6 mg), simple sugars (6-1507 mg), raffinose and fructooligosaccharides (0.8-169 mg), hemicellulose (77-763 mg), lignin (0-90 mg), and unknown dietary fiber (5-6 g).
31316128	5	86	theme	mineral	732:738	arg1	Cu					835:836	1-136 µg Cu	826:836	1-136 µg Cu	826:836	A single 100-g serving of fresh organic kale can provide mineral micronutrients (43-438 mg Ca; 11-60 mg Mg; 28-102 mg P; 0.5-3.3 mg Fe; 0.3-1.3 mg Mn; 1-136 µg Cu; and 0-35 µg Se) as well as 5.7-8.7 g of total prebiotic carbohydrates, including sugar alcohols (0.4-6.6 mg), simple sugars (6-1507 mg), raffinose and fructooligosaccharides (0.8-169 mg), hemicellulose (77-763 mg), lignin (0-90 mg), and unknown dietary fiber (5-6 g).
31316128	5	86	theme	mineral	732:738	arg1	Fe					807:808	0.5-3.3 mg Fe	796:808	0.5-3.3 mg Fe	796:808	A single 100-g serving of fresh organic kale can provide mineral micronutrients (43-438 mg Ca; 11-60 mg Mg; 28-102 mg P; 0.5-3.3 mg Fe; 0.3-1.3 mg Mn; 1-136 µg Cu; and 0-35 µg Se) as well as 5.7-8.7 g of total prebiotic carbohydrates, including sugar alcohols (0.4-6.6 mg), simple sugars (6-1507 mg), raffinose and fructooligosaccharides (0.8-169 mg), hemicellulose (77-763 mg), lignin (0-90 mg), and unknown dietary fiber (5-6 g).
31316128	5	86	theme	mineral	732:738	arg1	Mg					779:780	11-60 mg Mg	770:780	11-60 mg Mg	770:780	A single 100-g serving of fresh organic kale can provide mineral micronutrients (43-438 mg Ca; 11-60 mg Mg; 28-102 mg P; 0.5-3.3 mg Fe; 0.3-1.3 mg Mn; 1-136 µg Cu; and 0-35 µg Se) as well as 5.7-8.7 g of total prebiotic carbohydrates, including sugar alcohols (0.4-6.6 mg), simple sugars (6-1507 mg), raffinose and fructooligosaccharides (0.8-169 mg), hemicellulose (77-763 mg), lignin (0-90 mg), and unknown dietary fiber (5-6 g).
31316128	5	86	theme	mineral	732:738	arg1	micronutrients					740:753	mineral micronutrients	732:753	mineral micronutrients (43-438 mg Ca; 11-60 mg Mg; 28-102 mg P; 0.5-3.3 mg Fe; 0.3-1.3 mg Mn; 1-136 µg Cu; and 0-35 µg Se)	732:853	A single 100-g serving of fresh organic kale can provide mineral micronutrients (43-438 mg Ca; 11-60 mg Mg; 28-102 mg P; 0.5-3.3 mg Fe; 0.3-1.3 mg Mn; 1-136 µg Cu; and 0-35 µg Se) as well as 5.7-8.7 g of total prebiotic carbohydrates, including sugar alcohols (0.4-6.6 mg), simple sugars (6-1507 mg), raffinose and fructooligosaccharides (0.8-169 mg), hemicellulose (77-763 mg), lignin (0-90 mg), and unknown dietary fiber (5-6 g).
31316128	5	86	theme	mineral	732:738	arg1	Mn					822:823	0.3-1.3 mg Mn	811:823	0.3-1.3 mg Mn	811:823	A single 100-g serving of fresh organic kale can provide mineral micronutrients (43-438 mg Ca; 11-60 mg Mg; 28-102 mg P; 0.5-3.3 mg Fe; 0.3-1.3 mg Mn; 1-136 µg Cu; and 0-35 µg Se) as well as 5.7-8.7 g of total prebiotic carbohydrates, including sugar alcohols (0.4-6.6 mg), simple sugars (6-1507 mg), raffinose and fructooligosaccharides (0.8-169 mg), hemicellulose (77-763 mg), lignin (0-90 mg), and unknown dietary fiber (5-6 g).
31316128	2	87	theme	leafy	126:130	arg1	vegetable					138:146	a leafy green vegetable	124:146	a leafy green vegetable regularly grown using non-organic agricultural systems	124:201	Kale is a leafy green vegetable regularly grown using non-organic agricultural systems.
31316128	2	87	theme	leafy	126:130	arg1	Kale					116:119	Kale	116:119	Kale	116:119	Kale is a leafy green vegetable regularly grown using non-organic agricultural systems.
31316128	8	88	from	concentrations	1466:1479	arg1	crops					1500:1504	subsequent kale crops	1484:1504	subsequent kale crops	1484:1504	Among the cover crops, faba bean results in the highest mineral, protein, and prebiotic carbohydrate concentrations in subsequent kale crops but ryegrass increases kale biomass production.
31316128	8	89	theme	carbohydrate	1453:1464	arg1	concentrations					1466:1479	the highest mineral, protein, and prebiotic carbohydrate concentrations	1409:1479	concentrations	1466:1479	Among the cover crops, faba bean results in the highest mineral, protein, and prebiotic carbohydrate concentrations in subsequent kale crops but ryegrass increases kale biomass production.
31316128	2	90	theme	non-organic	170:180	arg1	systems					195:201	non-organic agricultural systems	170:201	non-organic agricultural systems	170:201	Kale is a leafy green vegetable regularly grown using non-organic agricultural systems.
31316128	8	91	theme	biomass	1534:1540	arg1	production					1542:1551	kale biomass production	1529:1551	kale biomass production	1529:1551	Among the cover crops, faba bean results in the highest mineral, protein, and prebiotic carbohydrate concentrations in subsequent kale crops but ryegrass increases kale biomass production.
31316128	4	92	theme	organic	558:564	arg1	kale					566:569	organic kale	558:569	organic kale	558:569	The objective of this study was to determine the effect of organic cover cropping systems on subsequent kale biomass production and nutrient composition (protein, mineral, and prebiotic carbohydrate concentrations) and to assess organic kale as a potential whole food source of daily essential mineral micronutrients and prebiotic carbohydrates.
31316128	4	92	theme	organic	558:564	arg1	source					597:602	a potential whole food source	574:602	a potential whole food source of daily essential mineral micronutrients and prebiotic carbohydrates	574:672	The objective of this study was to determine the effect of organic cover cropping systems on subsequent kale biomass production and nutrient composition (protein, mineral, and prebiotic carbohydrate concentrations) and to assess organic kale as a potential whole food source of daily essential mineral micronutrients and prebiotic carbohydrates.
31316128	5	93	theme	sugar	920:924	arg1	0.4-6.6 mg					936:945	0.4-6.6 mg	936:945	0.4-6.6 mg	936:945	A single 100-g serving of fresh organic kale can provide mineral micronutrients (43-438 mg Ca; 11-60 mg Mg; 28-102 mg P; 0.5-3.3 mg Fe; 0.3-1.3 mg Mn; 1-136 µg Cu; and 0-35 µg Se) as well as 5.7-8.7 g of total prebiotic carbohydrates, including sugar alcohols (0.4-6.6 mg), simple sugars (6-1507 mg), raffinose and fructooligosaccharides (0.8-169 mg), hemicellulose (77-763 mg), lignin (0-90 mg), and unknown dietary fiber (5-6 g).
31316128	5	93	theme	sugar	920:924	arg1	alcohols					926:933	sugar alcohols	920:933	sugar alcohols (0.4-6.6 mg)	920:946	A single 100-g serving of fresh organic kale can provide mineral micronutrients (43-438 mg Ca; 11-60 mg Mg; 28-102 mg P; 0.5-3.3 mg Fe; 0.3-1.3 mg Mn; 1-136 µg Cu; and 0-35 µg Se) as well as 5.7-8.7 g of total prebiotic carbohydrates, including sugar alcohols (0.4-6.6 mg), simple sugars (6-1507 mg), raffinose and fructooligosaccharides (0.8-169 mg), hemicellulose (77-763 mg), lignin (0-90 mg), and unknown dietary fiber (5-6 g).
31316128	8	94	theme	kale	1495:1498	arg1	crops					1500:1504	subsequent kale crops	1484:1504	subsequent kale crops	1484:1504	Among the cover crops, faba bean results in the highest mineral, protein, and prebiotic carbohydrate concentrations in subsequent kale crops but ryegrass increases kale biomass production.
31279287	4	0	theme	uniform	1043:1049	arg1	structure					1058:1066	asymmetric and open uniform porous structure	1023:1066	asymmetric and open uniform porous structure	1023:1066	The ultra-small GQDs loaded Dex/PNIPAM and was appeared to be asymmetric and open uniform porous structure, which can be significantly favorable for cell uptake and greatly influenced to be an effective drug carrier into the cellular compartment with good fluid flow.
31279287	4	0	theme	uniform	1043:1049	arg1	GQDs					977:980	The ultra-small GQDs	961:980	The ultra-small GQDs	961:980	The ultra-small GQDs loaded Dex/PNIPAM and was appeared to be asymmetric and open uniform porous structure, which can be significantly favorable for cell uptake and greatly influenced to be an effective drug carrier into the cellular compartment with good fluid flow.
31279287	2	1	theme	dots	693:696	arg1	presence					664:671	presence	664:671	presence of graphene quantum dots to achieve thermal responsive drug delivery (TrDD) with no harm effect in the implantation site	664:792	This research report was aimed to grafting and functionalizing thermo-responsive (Dex/PNIPAM) copolymeric composite with presence of graphene quantum dots to achieve thermal responsive drug delivery (TrDD) with no harm effect in the implantation site.
31279287	2	2	theme	copolymeric	637:647	arg1	composite					649:657	thermo-responsive (Dex/PNIPAM) copolymeric composite	606:657	thermo-responsive (Dex/PNIPAM) copolymeric composite	606:657	This research report was aimed to grafting and functionalizing thermo-responsive (Dex/PNIPAM) copolymeric composite with presence of graphene quantum dots to achieve thermal responsive drug delivery (TrDD) with no harm effect in the implantation site.
31279287	5	3	theme	release	1303:1309	arg1	percentages					1311:1321	drug release percentages	1298:1321	drug release percentages	1298:1321	The PNIPAM polymeric composite were exhibited sustained and enhanced drug release percentages with increasing temperature at above low critical solution temperature (LCST) is 39 °C comparable to the cumulative drug release profile of below LCST (32 °C), which demonstrated that thermo-responsive polymer was played a significant role in the delivery system.
31279287	1	4	theme	dots	311:314	arg1	N-isopropylacrylamide					343:363	N-isopropylacrylamide	343:363	N-isopropylacrylamide	343:363	A novel nano-formulations of biocompatible, biodegradable and thermo-responsive graphene quantum dots (GQDs) loaded dextran/poly(N-isopropylacrylamide) (Dex/PNIPAM) copolymeric matrix was synthesized and analyzed the materials characterization, sustained drug delivery system, tissue feasibility in the tissue implantation site.
31279287	1	4	theme	dots	311:314	arg1	nano-formulations					222:238	A novel nano-formulations	214:238	A novel nano-formulations of biocompatible, biodegradable and thermo-responsive graphene quantum dots (GQDs) loaded dextran/poly(N-isopropylacrylamide)	214:364	A novel nano-formulations of biocompatible, biodegradable and thermo-responsive graphene quantum dots (GQDs) loaded dextran/poly(N-isopropylacrylamide) (Dex/PNIPAM) copolymeric matrix was synthesized and analyzed the materials characterization, sustained drug delivery system, tissue feasibility in the tissue implantation site.
31279287	0	5	theme	dextran/glycol-polymeric	80:103	arg1	hydrogel					105:112	dextran/glycol-polymeric hydrogel	80:112	dextran/glycol-polymeric hydrogel for thermoresponsive triggered delivery of buprenorphine	80:169	Facile design and development of photoluminescent graphene quantum dots grafted dextran/glycol-polymeric hydrogel for thermoresponsive triggered delivery of buprenorphine on pain management in tissue implantation.
31279287	2	6	theme	quantum	685:691	arg1	dots					693:696	graphene quantum dots	676:696	graphene quantum dots	676:696	This research report was aimed to grafting and functionalizing thermo-responsive (Dex/PNIPAM) copolymeric composite with presence of graphene quantum dots to achieve thermal responsive drug delivery (TrDD) with no harm effect in the implantation site.
31279287	5	7	theme	significant	1546:1556	arg1	role					1558:1561	a significant role	1544:1561	a significant role	1544:1561	The PNIPAM polymeric composite were exhibited sustained and enhanced drug release percentages with increasing temperature at above low critical solution temperature (LCST) is 39 °C comparable to the cumulative drug release profile of below LCST (32 °C), which demonstrated that thermo-responsive polymer was played a significant role in the delivery system.
31279287	4	8	theme	cell	1110:1113	arg1	uptake					1115:1120	cell uptake	1110:1120	cell uptake	1110:1120	The ultra-small GQDs loaded Dex/PNIPAM and was appeared to be asymmetric and open uniform porous structure, which can be significantly favorable for cell uptake and greatly influenced to be an effective drug carrier into the cellular compartment with good fluid flow.
31279287	5	9	theme	polymeric	1240:1248	arg1	composite					1250:1258	The PNIPAM polymeric composite	1229:1258	The PNIPAM polymeric composite	1229:1258	The PNIPAM polymeric composite were exhibited sustained and enhanced drug release percentages with increasing temperature at above low critical solution temperature (LCST) is 39 °C comparable to the cumulative drug release profile of below LCST (32 °C), which demonstrated that thermo-responsive polymer was played a significant role in the delivery system.
31279287	0	10	theme	thermoresponsive	118:133	arg1	delivery					145:152	thermoresponsive triggered delivery	118:152	thermoresponsive triggered delivery of buprenorphine	118:169	Facile design and development of photoluminescent graphene quantum dots grafted dextran/glycol-polymeric hydrogel for thermoresponsive triggered delivery of buprenorphine on pain management in tissue implantation.
31279287	2	11	theme	graphene	676:683	arg1	dots					693:696	graphene quantum dots	676:696	graphene quantum dots	676:696	This research report was aimed to grafting and functionalizing thermo-responsive (Dex/PNIPAM) copolymeric composite with presence of graphene quantum dots to achieve thermal responsive drug delivery (TrDD) with no harm effect in the implantation site.
31279287	4	12	theme	asymmetric	1023:1032	arg1	structure					1058:1066	asymmetric and open uniform porous structure	1023:1066	asymmetric and open uniform porous structure	1023:1066	The ultra-small GQDs loaded Dex/PNIPAM and was appeared to be asymmetric and open uniform porous structure, which can be significantly favorable for cell uptake and greatly influenced to be an effective drug carrier into the cellular compartment with good fluid flow.
31279287	4	12	theme	asymmetric	1023:1032	arg1	GQDs					977:980	The ultra-small GQDs	961:980	The ultra-small GQDs	961:980	The ultra-small GQDs loaded Dex/PNIPAM and was appeared to be asymmetric and open uniform porous structure, which can be significantly favorable for cell uptake and greatly influenced to be an effective drug carrier into the cellular compartment with good fluid flow.
31279287	5	13	theme	drug	1439:1442	arg1	profile					1452:1458	the cumulative drug release profile	1424:1458	the cumulative drug release profile of below LCST (32 °C), which demonstrated that thermo-responsive polymer was played a significant role in the delivery system	1424:1584	The PNIPAM polymeric composite were exhibited sustained and enhanced drug release percentages with increasing temperature at above low critical solution temperature (LCST) is 39 °C comparable to the cumulative drug release profile of below LCST (32 °C), which demonstrated that thermo-responsive polymer was played a significant role in the delivery system.
31279287	5	14	theme	solution	1373:1380	arg1	LCST					1395:1398	LCST	1395:1398	LCST	1395:1398	The PNIPAM polymeric composite were exhibited sustained and enhanced drug release percentages with increasing temperature at above low critical solution temperature (LCST) is 39 °C comparable to the cumulative drug release profile of below LCST (32 °C), which demonstrated that thermo-responsive polymer was played a significant role in the delivery system.
31279287	5	14	theme	solution	1373:1380	arg1	temperature					1382:1392	above low critical solution temperature	1354:1392	above low critical solution temperature (LCST)	1354:1399	The PNIPAM polymeric composite were exhibited sustained and enhanced drug release percentages with increasing temperature at above low critical solution temperature (LCST) is 39 °C comparable to the cumulative drug release profile of below LCST (32 °C), which demonstrated that thermo-responsive polymer was played a significant role in the delivery system.
31279287	6	15	theme	treated	1591:1597	arg1	GQDs-Dex/PNIPAM					1608:1622	GQDs-Dex/PNIPAM	1608:1622	GQDs-Dex/PNIPAM	1608:1622	The treated group of GQDs-Dex/PNIPAM was observed that no inflammation and shows noteworthy stromal cell infiltration, demonstrating that the synthesized drug carriers did not harm to the nerves and tissues and only was responsible for the pain management.
31279287	6	15	theme	treated	1591:1597	arg1	group					1599:1603	The treated group	1587:1603	The treated group of GQDs-Dex/PNIPAM	1587:1622	The treated group of GQDs-Dex/PNIPAM was observed that no inflammation and shows noteworthy stromal cell infiltration, demonstrating that the synthesized drug carriers did not harm to the nerves and tissues and only was responsible for the pain management.
31279287	0	16	from	management	179:188	arg1	implantation					200:211	tissue implantation	193:211	tissue implantation	193:211	Facile design and development of photoluminescent graphene quantum dots grafted dextran/glycol-polymeric hydrogel for thermoresponsive triggered delivery of buprenorphine on pain management in tissue implantation.
31279287	4	17	with	compartment	1195:1205	arg1	flow					1223:1226	good fluid flow	1212:1226	good fluid flow	1212:1226	The ultra-small GQDs loaded Dex/PNIPAM and was appeared to be asymmetric and open uniform porous structure, which can be significantly favorable for cell uptake and greatly influenced to be an effective drug carrier into the cellular compartment with good fluid flow.
31279287	3	18	theme	synthesized	799:809	arg1	GQD					811:813	The synthesized GQD	795:813	The synthesized GQD by using ionic liquid	795:835	The synthesized GQD by using ionic liquid were evaluated by spectroscopic (DLS, PL, XRD and Raman spectroscopy) and Transmission electron microscopic analysis (TEM).
31279287	2	19	theme	responsive	717:726	arg1	delivery					733:740	thermal responsive drug delivery	709:740	thermal responsive drug delivery	709:740	This research report was aimed to grafting and functionalizing thermo-responsive (Dex/PNIPAM) copolymeric composite with presence of graphene quantum dots to achieve thermal responsive drug delivery (TrDD) with no harm effect in the implantation site.
31279287	1	20	theme	tissue	517:522	arg1	site					537:540	the tissue implantation site	513:540	the tissue implantation site	513:540	A novel nano-formulations of biocompatible, biodegradable and thermo-responsive graphene quantum dots (GQDs) loaded dextran/poly(N-isopropylacrylamide) (Dex/PNIPAM) copolymeric matrix was synthesized and analyzed the materials characterization, sustained drug delivery system, tissue feasibility in the tissue implantation site.
31279287	4	21	theme	drug	1164:1167	arg1	carrier					1169:1175	an effective drug carrier	1151:1175	an effective drug carrier into the cellular compartment with good fluid flow	1151:1226	The ultra-small GQDs loaded Dex/PNIPAM and was appeared to be asymmetric and open uniform porous structure, which can be significantly favorable for cell uptake and greatly influenced to be an effective drug carrier into the cellular compartment with good fluid flow.
31279287	0	22	theme	triggered	135:143	arg1	delivery					145:152	thermoresponsive triggered delivery	118:152	thermoresponsive triggered delivery of buprenorphine	118:169	Facile design and development of photoluminescent graphene quantum dots grafted dextran/glycol-polymeric hydrogel for thermoresponsive triggered delivery of buprenorphine on pain management in tissue implantation.
31279287	6	23	theme	drug	1741:1744	arg1	responsible					1807:1817	responsible	1807:1817	responsible	1807:1817	The treated group of GQDs-Dex/PNIPAM was observed that no inflammation and shows noteworthy stromal cell infiltration, demonstrating that the synthesized drug carriers did not harm to the nerves and tissues and only was responsible for the pain management.
31279287	6	23	theme	drug	1741:1744	arg1	carriers					1746:1753	the synthesized drug carriers	1725:1753	the synthesized drug carriers	1725:1753	The treated group of GQDs-Dex/PNIPAM was observed that no inflammation and shows noteworthy stromal cell infiltration, demonstrating that the synthesized drug carriers did not harm to the nerves and tissues and only was responsible for the pain management.
31279287	2	24	theme	thermal	709:715	arg1	delivery					733:740	thermal responsive drug delivery	709:740	thermal responsive drug delivery	709:740	This research report was aimed to grafting and functionalizing thermo-responsive (Dex/PNIPAM) copolymeric composite with presence of graphene quantum dots to achieve thermal responsive drug delivery (TrDD) with no harm effect in the implantation site.
31279287	1	25	theme	implantation	524:535	arg1	site					537:540	the tissue implantation site	513:540	the tissue implantation site	513:540	A novel nano-formulations of biocompatible, biodegradable and thermo-responsive graphene quantum dots (GQDs) loaded dextran/poly(N-isopropylacrylamide) (Dex/PNIPAM) copolymeric matrix was synthesized and analyzed the materials characterization, sustained drug delivery system, tissue feasibility in the tissue implantation site.
31279287	1	26	from	materials	431:439	arg1	site					537:540	the tissue implantation site	513:540	the tissue implantation site	513:540	A novel nano-formulations of biocompatible, biodegradable and thermo-responsive graphene quantum dots (GQDs) loaded dextran/poly(N-isopropylacrylamide) (Dex/PNIPAM) copolymeric matrix was synthesized and analyzed the materials characterization, sustained drug delivery system, tissue feasibility in the tissue implantation site.
31279287	2	27	from	effect	762:767	arg1	site					789:792	the implantation site	772:792	the implantation site	772:792	This research report was aimed to grafting and functionalizing thermo-responsive (Dex/PNIPAM) copolymeric composite with presence of graphene quantum dots to achieve thermal responsive drug delivery (TrDD) with no harm effect in the implantation site.
31279287	2	28	dep	achieve	701:707	arg1	TrDD					743:746	TrDD	743:746	TrDD	743:746	This research report was aimed to grafting and functionalizing thermo-responsive (Dex/PNIPAM) copolymeric composite with presence of graphene quantum dots to achieve thermal responsive drug delivery (TrDD) with no harm effect in the implantation site.
31279287	2	29	theme	research	548:555	arg1	report					557:562	This research report	543:562	This research report	543:562	This research report was aimed to grafting and functionalizing thermo-responsive (Dex/PNIPAM) copolymeric composite with presence of graphene quantum dots to achieve thermal responsive drug delivery (TrDD) with no harm effect in the implantation site.
31279287	5	30	theme	thermo-responsive	1507:1523	arg1	polymer					1525:1531	thermo-responsive polymer	1507:1531	thermo-responsive polymer	1507:1531	The PNIPAM polymeric composite were exhibited sustained and enhanced drug release percentages with increasing temperature at above low critical solution temperature (LCST) is 39 °C comparable to the cumulative drug release profile of below LCST (32 °C), which demonstrated that thermo-responsive polymer was played a significant role in the delivery system.
31279287	6	31	theme	pain	1827:1830	arg1	management					1832:1841	the pain management	1823:1841	the pain management	1823:1841	The treated group of GQDs-Dex/PNIPAM was observed that no inflammation and shows noteworthy stromal cell infiltration, demonstrating that the synthesized drug carriers did not harm to the nerves and tissues and only was responsible for the pain management.
31279287	1	32	theme	novel	216:220	arg1	N-isopropylacrylamide					343:363	N-isopropylacrylamide	343:363	N-isopropylacrylamide	343:363	A novel nano-formulations of biocompatible, biodegradable and thermo-responsive graphene quantum dots (GQDs) loaded dextran/poly(N-isopropylacrylamide) (Dex/PNIPAM) copolymeric matrix was synthesized and analyzed the materials characterization, sustained drug delivery system, tissue feasibility in the tissue implantation site.
31279287	1	32	theme	novel	216:220	arg1	nano-formulations					222:238	A novel nano-formulations	214:238	A novel nano-formulations of biocompatible, biodegradable and thermo-responsive graphene quantum dots (GQDs) loaded dextran/poly(N-isopropylacrylamide)	214:364	A novel nano-formulations of biocompatible, biodegradable and thermo-responsive graphene quantum dots (GQDs) loaded dextran/poly(N-isopropylacrylamide) (Dex/PNIPAM) copolymeric matrix was synthesized and analyzed the materials characterization, sustained drug delivery system, tissue feasibility in the tissue implantation site.
31279287	0	33	theme	Facile	0:5	arg1	design					7:12	Facile design	0:12	Facile design	0:12	Facile design and development of photoluminescent graphene quantum dots grafted dextran/glycol-polymeric hydrogel for thermoresponsive triggered delivery of buprenorphine on pain management in tissue implantation.
31279287	5	34	theme	PNIPAM	1233:1238	arg1	composite					1250:1258	The PNIPAM polymeric composite	1229:1258	The PNIPAM polymeric composite	1229:1258	The PNIPAM polymeric composite were exhibited sustained and enhanced drug release percentages with increasing temperature at above low critical solution temperature (LCST) is 39 °C comparable to the cumulative drug release profile of below LCST (32 °C), which demonstrated that thermo-responsive polymer was played a significant role in the delivery system.
31279287	5	35	theme	delivery	1570:1577	arg1	system					1579:1584	the delivery system	1566:1584	the delivery system	1566:1584	The PNIPAM polymeric composite were exhibited sustained and enhanced drug release percentages with increasing temperature at above low critical solution temperature (LCST) is 39 °C comparable to the cumulative drug release profile of below LCST (32 °C), which demonstrated that thermo-responsive polymer was played a significant role in the delivery system.
31279287	5	36	theme	low	1360:1362	arg1	LCST					1395:1398	LCST	1395:1398	LCST	1395:1398	The PNIPAM polymeric composite were exhibited sustained and enhanced drug release percentages with increasing temperature at above low critical solution temperature (LCST) is 39 °C comparable to the cumulative drug release profile of below LCST (32 °C), which demonstrated that thermo-responsive polymer was played a significant role in the delivery system.
31279287	5	36	theme	low	1360:1362	arg1	temperature					1382:1392	above low critical solution temperature	1354:1392	above low critical solution temperature (LCST)	1354:1399	The PNIPAM polymeric composite were exhibited sustained and enhanced drug release percentages with increasing temperature at above low critical solution temperature (LCST) is 39 °C comparable to the cumulative drug release profile of below LCST (32 °C), which demonstrated that thermo-responsive polymer was played a significant role in the delivery system.
31279287	0	37	theme	buprenorphine	157:169	arg1	delivery					145:152	thermoresponsive triggered delivery	118:152	thermoresponsive triggered delivery of buprenorphine	118:169	Facile design and development of photoluminescent graphene quantum dots grafted dextran/glycol-polymeric hydrogel for thermoresponsive triggered delivery of buprenorphine on pain management in tissue implantation.
31279287	2	38	theme	drug	728:731	arg1	delivery					733:740	thermal responsive drug delivery	709:740	thermal responsive drug delivery	709:740	This research report was aimed to grafting and functionalizing thermo-responsive (Dex/PNIPAM) copolymeric composite with presence of graphene quantum dots to achieve thermal responsive drug delivery (TrDD) with no harm effect in the implantation site.
31279287	5	39	theme	critical	1364:1371	arg1	LCST					1395:1398	LCST	1395:1398	LCST	1395:1398	The PNIPAM polymeric composite were exhibited sustained and enhanced drug release percentages with increasing temperature at above low critical solution temperature (LCST) is 39 °C comparable to the cumulative drug release profile of below LCST (32 °C), which demonstrated that thermo-responsive polymer was played a significant role in the delivery system.
31279287	5	39	theme	critical	1364:1371	arg1	temperature					1382:1392	above low critical solution temperature	1354:1392	above low critical solution temperature (LCST)	1354:1399	The PNIPAM polymeric composite were exhibited sustained and enhanced drug release percentages with increasing temperature at above low critical solution temperature (LCST) is 39 °C comparable to the cumulative drug release profile of below LCST (32 °C), which demonstrated that thermo-responsive polymer was played a significant role in the delivery system.
31279287	6	40	theme	GQDs-Dex/PNIPAM	1608:1622	arg1	GQDs-Dex/PNIPAM					1608:1622	GQDs-Dex/PNIPAM	1608:1622	GQDs-Dex/PNIPAM	1608:1622	The treated group of GQDs-Dex/PNIPAM was observed that no inflammation and shows noteworthy stromal cell infiltration, demonstrating that the synthesized drug carriers did not harm to the nerves and tissues and only was responsible for the pain management.
31279287	6	40	theme	GQDs-Dex/PNIPAM	1608:1622	arg1	group					1599:1603	The treated group	1587:1603	The treated group of GQDs-Dex/PNIPAM	1587:1622	The treated group of GQDs-Dex/PNIPAM was observed that no inflammation and shows noteworthy stromal cell infiltration, demonstrating that the synthesized drug carriers did not harm to the nerves and tissues and only was responsible for the pain management.
31279287	1	41	dep	copolymeric	379:389	arg1	N-isopropylacrylamide					343:363	N-isopropylacrylamide	343:363	N-isopropylacrylamide	343:363	A novel nano-formulations of biocompatible, biodegradable and thermo-responsive graphene quantum dots (GQDs) loaded dextran/poly(N-isopropylacrylamide) (Dex/PNIPAM) copolymeric matrix was synthesized and analyzed the materials characterization, sustained drug delivery system, tissue feasibility in the tissue implantation site.
31279287	1	41	dep	copolymeric	379:389	arg1	nano-formulations					222:238	A novel nano-formulations	214:238	A novel nano-formulations of biocompatible, biodegradable and thermo-responsive graphene quantum dots (GQDs) loaded dextran/poly(N-isopropylacrylamide)	214:364	A novel nano-formulations of biocompatible, biodegradable and thermo-responsive graphene quantum dots (GQDs) loaded dextran/poly(N-isopropylacrylamide) (Dex/PNIPAM) copolymeric matrix was synthesized and analyzed the materials characterization, sustained drug delivery system, tissue feasibility in the tissue implantation site.
31279287	2	42	theme	Dex/PNIPAM	625:634	arg1	composite					649:657	thermo-responsive (Dex/PNIPAM) copolymeric composite	606:657	thermo-responsive (Dex/PNIPAM) copolymeric composite	606:657	This research report was aimed to grafting and functionalizing thermo-responsive (Dex/PNIPAM) copolymeric composite with presence of graphene quantum dots to achieve thermal responsive drug delivery (TrDD) with no harm effect in the implantation site.
31279287	3	43	dep	spectroscopic	855:867	arg1	DLS					870:872	DLS	870:872	DLS	870:872	The synthesized GQD by using ionic liquid were evaluated by spectroscopic (DLS, PL, XRD and Raman spectroscopy) and Transmission electron microscopic analysis (TEM).
31279287	3	43	dep	spectroscopic	855:867	arg1	XRD					879:881	XRD	879:881	XRD	879:881	The synthesized GQD by using ionic liquid were evaluated by spectroscopic (DLS, PL, XRD and Raman spectroscopy) and Transmission electron microscopic analysis (TEM).
31279287	3	43	dep	spectroscopic	855:867	arg1	spectroscopy					893:904	Raman spectroscopy	887:904	Raman spectroscopy	887:904	The synthesized GQD by using ionic liquid were evaluated by spectroscopic (DLS, PL, XRD and Raman spectroscopy) and Transmission electron microscopic analysis (TEM).
31279287	3	43	dep	spectroscopic	855:867	arg1	PL					875:876	PL	875:876	PL	875:876	The synthesized GQD by using ionic liquid were evaluated by spectroscopic (DLS, PL, XRD and Raman spectroscopy) and Transmission electron microscopic analysis (TEM).
31279287	4	44	theme	effective	1154:1162	arg1	carrier					1169:1175	an effective drug carrier	1151:1175	an effective drug carrier into the cellular compartment with good fluid flow	1151:1226	The ultra-small GQDs loaded Dex/PNIPAM and was appeared to be asymmetric and open uniform porous structure, which can be significantly favorable for cell uptake and greatly influenced to be an effective drug carrier into the cellular compartment with good fluid flow.
31279287	1	45	theme	biocompatible	243:255	arg1	GQDs					317:320	GQDs	317:320	GQDs	317:320	A novel nano-formulations of biocompatible, biodegradable and thermo-responsive graphene quantum dots (GQDs) loaded dextran/poly(N-isopropylacrylamide) (Dex/PNIPAM) copolymeric matrix was synthesized and analyzed the materials characterization, sustained drug delivery system, tissue feasibility in the tissue implantation site.
31279287	1	45	theme	biocompatible	243:255	arg1	dots					311:314	biocompatible, biodegradable and thermo-responsive graphene quantum dots	243:314	biocompatible, biodegradable and thermo-responsive graphene quantum dots (GQDs) loaded dextran/poly	243:341	A novel nano-formulations of biocompatible, biodegradable and thermo-responsive graphene quantum dots (GQDs) loaded dextran/poly(N-isopropylacrylamide) (Dex/PNIPAM) copolymeric matrix was synthesized and analyzed the materials characterization, sustained drug delivery system, tissue feasibility in the tissue implantation site.
31279287	0	46	theme	photoluminescent	33:48	arg1	dots					67:70	photoluminescent graphene quantum dots	33:70	photoluminescent graphene quantum dots	33:70	Facile design and development of photoluminescent graphene quantum dots grafted dextran/glycol-polymeric hydrogel for thermoresponsive triggered delivery of buprenorphine on pain management in tissue implantation.
31279287	0	47	theme	pain	174:177	arg1	management					179:188	pain management	174:188	pain management in tissue implantation	174:211	Facile design and development of photoluminescent graphene quantum dots grafted dextran/glycol-polymeric hydrogel for thermoresponsive triggered delivery of buprenorphine on pain management in tissue implantation.
31279287	4	48	theme	open	1038:1041	arg1	structure					1058:1066	asymmetric and open uniform porous structure	1023:1066	asymmetric and open uniform porous structure	1023:1066	The ultra-small GQDs loaded Dex/PNIPAM and was appeared to be asymmetric and open uniform porous structure, which can be significantly favorable for cell uptake and greatly influenced to be an effective drug carrier into the cellular compartment with good fluid flow.
31279287	4	48	theme	open	1038:1041	arg1	GQDs					977:980	The ultra-small GQDs	961:980	The ultra-small GQDs	961:980	The ultra-small GQDs loaded Dex/PNIPAM and was appeared to be asymmetric and open uniform porous structure, which can be significantly favorable for cell uptake and greatly influenced to be an effective drug carrier into the cellular compartment with good fluid flow.
31279287	4	49	theme	porous	1051:1056	arg1	structure					1058:1066	asymmetric and open uniform porous structure	1023:1066	asymmetric and open uniform porous structure	1023:1066	The ultra-small GQDs loaded Dex/PNIPAM and was appeared to be asymmetric and open uniform porous structure, which can be significantly favorable for cell uptake and greatly influenced to be an effective drug carrier into the cellular compartment with good fluid flow.
31279287	4	49	theme	porous	1051:1056	arg1	GQDs					977:980	The ultra-small GQDs	961:980	The ultra-small GQDs	961:980	The ultra-small GQDs loaded Dex/PNIPAM and was appeared to be asymmetric and open uniform porous structure, which can be significantly favorable for cell uptake and greatly influenced to be an effective drug carrier into the cellular compartment with good fluid flow.
31279287	2	50	theme	thermo-responsive	606:622	arg1	composite					649:657	thermo-responsive (Dex/PNIPAM) copolymeric composite	606:657	thermo-responsive (Dex/PNIPAM) copolymeric composite	606:657	This research report was aimed to grafting and functionalizing thermo-responsive (Dex/PNIPAM) copolymeric composite with presence of graphene quantum dots to achieve thermal responsive drug delivery (TrDD) with no harm effect in the implantation site.
31279287	1	51	theme	sustained	459:467	arg1	system					483:488	sustained drug delivery system	459:488	sustained drug delivery system	459:488	A novel nano-formulations of biocompatible, biodegradable and thermo-responsive graphene quantum dots (GQDs) loaded dextran/poly(N-isopropylacrylamide) (Dex/PNIPAM) copolymeric matrix was synthesized and analyzed the materials characterization, sustained drug delivery system, tissue feasibility in the tissue implantation site.
31279287	1	51	theme	sustained	459:467	arg1	characterization					441:456	characterization	441:456	characterization	441:456	A novel nano-formulations of biocompatible, biodegradable and thermo-responsive graphene quantum dots (GQDs) loaded dextran/poly(N-isopropylacrylamide) (Dex/PNIPAM) copolymeric matrix was synthesized and analyzed the materials characterization, sustained drug delivery system, tissue feasibility in the tissue implantation site.
31279287	3	52	theme	spectroscopic	855:867	arg1	TEM					955:957	TEM	955:957	TEM	955:957	The synthesized GQD by using ionic liquid were evaluated by spectroscopic (DLS, PL, XRD and Raman spectroscopy) and Transmission electron microscopic analysis (TEM).
31279287	3	52	theme	spectroscopic	855:867	arg1	analysis					945:952	spectroscopic (DLS, PL, XRD and Raman spectroscopy) and Transmission electron microscopic analysis	855:952	spectroscopic (DLS, PL, XRD and Raman spectroscopy) and Transmission electron microscopic analysis (TEM)	855:958	The synthesized GQD by using ionic liquid were evaluated by spectroscopic (DLS, PL, XRD and Raman spectroscopy) and Transmission electron microscopic analysis (TEM).
31279287	2	53	theme	implantation	776:787	arg1	site					789:792	the implantation site	772:792	the implantation site	772:792	This research report was aimed to grafting and functionalizing thermo-responsive (Dex/PNIPAM) copolymeric composite with presence of graphene quantum dots to achieve thermal responsive drug delivery (TrDD) with no harm effect in the implantation site.
31279287	1	54	theme	biodegradable	258:270	arg1	GQDs					317:320	GQDs	317:320	GQDs	317:320	A novel nano-formulations of biocompatible, biodegradable and thermo-responsive graphene quantum dots (GQDs) loaded dextran/poly(N-isopropylacrylamide) (Dex/PNIPAM) copolymeric matrix was synthesized and analyzed the materials characterization, sustained drug delivery system, tissue feasibility in the tissue implantation site.
31279287	1	54	theme	biodegradable	258:270	arg1	dots					311:314	biocompatible, biodegradable and thermo-responsive graphene quantum dots	243:314	biocompatible, biodegradable and thermo-responsive graphene quantum dots (GQDs) loaded dextran/poly	243:341	A novel nano-formulations of biocompatible, biodegradable and thermo-responsive graphene quantum dots (GQDs) loaded dextran/poly(N-isopropylacrylamide) (Dex/PNIPAM) copolymeric matrix was synthesized and analyzed the materials characterization, sustained drug delivery system, tissue feasibility in the tissue implantation site.
31279287	0	55	theme	quantum	59:65	arg1	dots					67:70	photoluminescent graphene quantum dots	33:70	photoluminescent graphene quantum dots	33:70	Facile design and development of photoluminescent graphene quantum dots grafted dextran/glycol-polymeric hydrogel for thermoresponsive triggered delivery of buprenorphine on pain management in tissue implantation.
31279287	1	56	dep	materials	431:439	arg1	system					483:488	sustained drug delivery system	459:488	sustained drug delivery system	459:488	A novel nano-formulations of biocompatible, biodegradable and thermo-responsive graphene quantum dots (GQDs) loaded dextran/poly(N-isopropylacrylamide) (Dex/PNIPAM) copolymeric matrix was synthesized and analyzed the materials characterization, sustained drug delivery system, tissue feasibility in the tissue implantation site.
31279287	1	56	dep	materials	431:439	arg1	characterization					441:456	characterization	441:456	characterization	441:456	A novel nano-formulations of biocompatible, biodegradable and thermo-responsive graphene quantum dots (GQDs) loaded dextran/poly(N-isopropylacrylamide) (Dex/PNIPAM) copolymeric matrix was synthesized and analyzed the materials characterization, sustained drug delivery system, tissue feasibility in the tissue implantation site.
31279287	1	56	dep	materials	431:439	arg1	feasibility					498:508	tissue feasibility	491:508	tissue feasibility	491:508	A novel nano-formulations of biocompatible, biodegradable and thermo-responsive graphene quantum dots (GQDs) loaded dextran/poly(N-isopropylacrylamide) (Dex/PNIPAM) copolymeric matrix was synthesized and analyzed the materials characterization, sustained drug delivery system, tissue feasibility in the tissue implantation site.
31279287	1	57	theme	drug	469:472	arg1	system					483:488	sustained drug delivery system	459:488	sustained drug delivery system	459:488	A novel nano-formulations of biocompatible, biodegradable and thermo-responsive graphene quantum dots (GQDs) loaded dextran/poly(N-isopropylacrylamide) (Dex/PNIPAM) copolymeric matrix was synthesized and analyzed the materials characterization, sustained drug delivery system, tissue feasibility in the tissue implantation site.
31279287	1	57	theme	drug	469:472	arg1	characterization					441:456	characterization	441:456	characterization	441:456	A novel nano-formulations of biocompatible, biodegradable and thermo-responsive graphene quantum dots (GQDs) loaded dextran/poly(N-isopropylacrylamide) (Dex/PNIPAM) copolymeric matrix was synthesized and analyzed the materials characterization, sustained drug delivery system, tissue feasibility in the tissue implantation site.
31279287	4	58	theme	fluid	1217:1221	arg1	flow					1223:1226	good fluid flow	1212:1226	good fluid flow	1212:1226	The ultra-small GQDs loaded Dex/PNIPAM and was appeared to be asymmetric and open uniform porous structure, which can be significantly favorable for cell uptake and greatly influenced to be an effective drug carrier into the cellular compartment with good fluid flow.
31279287	5	59	theme	drug	1298:1301	arg1	percentages					1311:1321	drug release percentages	1298:1321	drug release percentages	1298:1321	The PNIPAM polymeric composite were exhibited sustained and enhanced drug release percentages with increasing temperature at above low critical solution temperature (LCST) is 39 °C comparable to the cumulative drug release profile of below LCST (32 °C), which demonstrated that thermo-responsive polymer was played a significant role in the delivery system.
31279287	0	60	theme	graphene	50:57	arg1	dots					67:70	photoluminescent graphene quantum dots	33:70	photoluminescent graphene quantum dots	33:70	Facile design and development of photoluminescent graphene quantum dots grafted dextran/glycol-polymeric hydrogel for thermoresponsive triggered delivery of buprenorphine on pain management in tissue implantation.
31279287	4	61	theme	ultra-small	965:975	arg1	structure					1058:1066	asymmetric and open uniform porous structure	1023:1066	asymmetric and open uniform porous structure	1023:1066	The ultra-small GQDs loaded Dex/PNIPAM and was appeared to be asymmetric and open uniform porous structure, which can be significantly favorable for cell uptake and greatly influenced to be an effective drug carrier into the cellular compartment with good fluid flow.
31279287	4	61	theme	ultra-small	965:975	arg1	GQDs					977:980	The ultra-small GQDs	961:980	The ultra-small GQDs	961:980	The ultra-small GQDs loaded Dex/PNIPAM and was appeared to be asymmetric and open uniform porous structure, which can be significantly favorable for cell uptake and greatly influenced to be an effective drug carrier into the cellular compartment with good fluid flow.
31279287	1	62	theme	delivery	474:481	arg1	system					483:488	sustained drug delivery system	459:488	sustained drug delivery system	459:488	A novel nano-formulations of biocompatible, biodegradable and thermo-responsive graphene quantum dots (GQDs) loaded dextran/poly(N-isopropylacrylamide) (Dex/PNIPAM) copolymeric matrix was synthesized and analyzed the materials characterization, sustained drug delivery system, tissue feasibility in the tissue implantation site.
31279287	1	62	theme	delivery	474:481	arg1	characterization					441:456	characterization	441:456	characterization	441:456	A novel nano-formulations of biocompatible, biodegradable and thermo-responsive graphene quantum dots (GQDs) loaded dextran/poly(N-isopropylacrylamide) (Dex/PNIPAM) copolymeric matrix was synthesized and analyzed the materials characterization, sustained drug delivery system, tissue feasibility in the tissue implantation site.
31279287	6	63	theme	synthesized	1729:1739	arg1	responsible					1807:1817	responsible	1807:1817	responsible	1807:1817	The treated group of GQDs-Dex/PNIPAM was observed that no inflammation and shows noteworthy stromal cell infiltration, demonstrating that the synthesized drug carriers did not harm to the nerves and tissues and only was responsible for the pain management.
31279287	6	63	theme	synthesized	1729:1739	arg1	carriers					1746:1753	the synthesized drug carriers	1725:1753	the synthesized drug carriers	1725:1753	The treated group of GQDs-Dex/PNIPAM was observed that no inflammation and shows noteworthy stromal cell infiltration, demonstrating that the synthesized drug carriers did not harm to the nerves and tissues and only was responsible for the pain management.
31279287	5	64	theme	release	1444:1450	arg1	profile					1452:1458	the cumulative drug release profile	1424:1458	the cumulative drug release profile of below LCST (32 °C), which demonstrated that thermo-responsive polymer was played a significant role in the delivery system	1424:1584	The PNIPAM polymeric composite were exhibited sustained and enhanced drug release percentages with increasing temperature at above low critical solution temperature (LCST) is 39 °C comparable to the cumulative drug release profile of below LCST (32 °C), which demonstrated that thermo-responsive polymer was played a significant role in the delivery system.
31279287	2	65	theme	harm	757:760	arg1	effect					762:767	no harm effect	754:767	no harm effect in the implantation site	754:792	This research report was aimed to grafting and functionalizing thermo-responsive (Dex/PNIPAM) copolymeric composite with presence of graphene quantum dots to achieve thermal responsive drug delivery (TrDD) with no harm effect in the implantation site.
31279287	0	66	theme	tissue	193:198	arg1	implantation					200:211	tissue implantation	193:211	tissue implantation	193:211	Facile design and development of photoluminescent graphene quantum dots grafted dextran/glycol-polymeric hydrogel for thermoresponsive triggered delivery of buprenorphine on pain management in tissue implantation.
31279287	6	67	theme	cell	1687:1690	arg1	infiltration					1692:1703	stromal cell infiltration	1679:1703	stromal cell infiltration	1679:1703	The treated group of GQDs-Dex/PNIPAM was observed that no inflammation and shows noteworthy stromal cell infiltration, demonstrating that the synthesized drug carriers did not harm to the nerves and tissues and only was responsible for the pain management.
31279287	5	68	theme	cumulative	1428:1437	arg1	profile					1452:1458	the cumulative drug release profile	1424:1458	the cumulative drug release profile of below LCST (32 °C), which demonstrated that thermo-responsive polymer was played a significant role in the delivery system	1424:1584	The PNIPAM polymeric composite were exhibited sustained and enhanced drug release percentages with increasing temperature at above low critical solution temperature (LCST) is 39 °C comparable to the cumulative drug release profile of below LCST (32 °C), which demonstrated that thermo-responsive polymer was played a significant role in the delivery system.
31279287	6	69	theme	stromal	1679:1685	arg1	infiltration					1692:1703	stromal cell infiltration	1679:1703	stromal cell infiltration	1679:1703	The treated group of GQDs-Dex/PNIPAM was observed that no inflammation and shows noteworthy stromal cell infiltration, demonstrating that the synthesized drug carriers did not harm to the nerves and tissues and only was responsible for the pain management.
31279287	3	70	theme	Raman	887:891	arg1	DLS					870:872	DLS	870:872	DLS	870:872	The synthesized GQD by using ionic liquid were evaluated by spectroscopic (DLS, PL, XRD and Raman spectroscopy) and Transmission electron microscopic analysis (TEM).
31279287	3	70	theme	Raman	887:891	arg1	spectroscopy					893:904	Raman spectroscopy	887:904	Raman spectroscopy	887:904	The synthesized GQD by using ionic liquid were evaluated by spectroscopic (DLS, PL, XRD and Raman spectroscopy) and Transmission electron microscopic analysis (TEM).
31279287	1	71	theme	thermo-responsive	276:292	arg1	GQDs					317:320	GQDs	317:320	GQDs	317:320	A novel nano-formulations of biocompatible, biodegradable and thermo-responsive graphene quantum dots (GQDs) loaded dextran/poly(N-isopropylacrylamide) (Dex/PNIPAM) copolymeric matrix was synthesized and analyzed the materials characterization, sustained drug delivery system, tissue feasibility in the tissue implantation site.
31279287	1	71	theme	thermo-responsive	276:292	arg1	dots					311:314	biocompatible, biodegradable and thermo-responsive graphene quantum dots	243:314	biocompatible, biodegradable and thermo-responsive graphene quantum dots (GQDs) loaded dextran/poly	243:341	A novel nano-formulations of biocompatible, biodegradable and thermo-responsive graphene quantum dots (GQDs) loaded dextran/poly(N-isopropylacrylamide) (Dex/PNIPAM) copolymeric matrix was synthesized and analyzed the materials characterization, sustained drug delivery system, tissue feasibility in the tissue implantation site.
31279287	1	72	theme	tissue	491:496	arg1	feasibility					498:508	tissue feasibility	491:508	tissue feasibility	491:508	A novel nano-formulations of biocompatible, biodegradable and thermo-responsive graphene quantum dots (GQDs) loaded dextran/poly(N-isopropylacrylamide) (Dex/PNIPAM) copolymeric matrix was synthesized and analyzed the materials characterization, sustained drug delivery system, tissue feasibility in the tissue implantation site.
31279287	1	72	theme	tissue	491:496	arg1	characterization					441:456	characterization	441:456	characterization	441:456	A novel nano-formulations of biocompatible, biodegradable and thermo-responsive graphene quantum dots (GQDs) loaded dextran/poly(N-isopropylacrylamide) (Dex/PNIPAM) copolymeric matrix was synthesized and analyzed the materials characterization, sustained drug delivery system, tissue feasibility in the tissue implantation site.
31279287	4	73	theme	cellular	1186:1193	arg1	compartment					1195:1205	the cellular compartment	1182:1205	the cellular compartment with good fluid flow	1182:1226	The ultra-small GQDs loaded Dex/PNIPAM and was appeared to be asymmetric and open uniform porous structure, which can be significantly favorable for cell uptake and greatly influenced to be an effective drug carrier into the cellular compartment with good fluid flow.
31279287	5	74	theme	above	1354:1358	arg1	LCST					1395:1398	LCST	1395:1398	LCST	1395:1398	The PNIPAM polymeric composite were exhibited sustained and enhanced drug release percentages with increasing temperature at above low critical solution temperature (LCST) is 39 °C comparable to the cumulative drug release profile of below LCST (32 °C), which demonstrated that thermo-responsive polymer was played a significant role in the delivery system.
31279287	5	74	theme	above	1354:1358	arg1	temperature					1382:1392	above low critical solution temperature	1354:1392	above low critical solution temperature (LCST)	1354:1399	The PNIPAM polymeric composite were exhibited sustained and enhanced drug release percentages with increasing temperature at above low critical solution temperature (LCST) is 39 °C comparable to the cumulative drug release profile of below LCST (32 °C), which demonstrated that thermo-responsive polymer was played a significant role in the delivery system.
31279287	1	75	theme	graphene	294:301	arg1	GQDs					317:320	GQDs	317:320	GQDs	317:320	A novel nano-formulations of biocompatible, biodegradable and thermo-responsive graphene quantum dots (GQDs) loaded dextran/poly(N-isopropylacrylamide) (Dex/PNIPAM) copolymeric matrix was synthesized and analyzed the materials characterization, sustained drug delivery system, tissue feasibility in the tissue implantation site.
31279287	1	75	theme	graphene	294:301	arg1	dots					311:314	biocompatible, biodegradable and thermo-responsive graphene quantum dots	243:314	biocompatible, biodegradable and thermo-responsive graphene quantum dots (GQDs) loaded dextran/poly	243:341	A novel nano-formulations of biocompatible, biodegradable and thermo-responsive graphene quantum dots (GQDs) loaded dextran/poly(N-isopropylacrylamide) (Dex/PNIPAM) copolymeric matrix was synthesized and analyzed the materials characterization, sustained drug delivery system, tissue feasibility in the tissue implantation site.
31279287	0	76	theme	dots	67:70	arg1	development					18:28	development	18:28	development	18:28	Facile design and development of photoluminescent graphene quantum dots grafted dextran/glycol-polymeric hydrogel for thermoresponsive triggered delivery of buprenorphine on pain management in tissue implantation.
31279287	0	76	theme	dots	67:70	arg1	design					7:12	Facile design	0:12	Facile design	0:12	Facile design and development of photoluminescent graphene quantum dots grafted dextran/glycol-polymeric hydrogel for thermoresponsive triggered delivery of buprenorphine on pain management in tissue implantation.
31279287	1	77	theme	copolymeric	379:389	arg1	matrix					391:396	A novel nano-formulations of biocompatible, biodegradable and thermo-responsive graphene quantum dots (GQDs) loaded dextran/poly(N-isopropylacrylamide) (Dex/PNIPAM) copolymeric matrix	214:396	A novel nano-formulations of biocompatible, biodegradable and thermo-responsive graphene quantum dots (GQDs) loaded dextran/poly(N-isopropylacrylamide) (Dex/PNIPAM) copolymeric matrix	214:396	A novel nano-formulations of biocompatible, biodegradable and thermo-responsive graphene quantum dots (GQDs) loaded dextran/poly(N-isopropylacrylamide) (Dex/PNIPAM) copolymeric matrix was synthesized and analyzed the materials characterization, sustained drug delivery system, tissue feasibility in the tissue implantation site.
31279287	4	78	theme	good	1212:1215	arg1	flow					1223:1226	good fluid flow	1212:1226	good fluid flow	1212:1226	The ultra-small GQDs loaded Dex/PNIPAM and was appeared to be asymmetric and open uniform porous structure, which can be significantly favorable for cell uptake and greatly influenced to be an effective drug carrier into the cellular compartment with good fluid flow.
31279287	3	79	theme	microscopic	933:943	arg1	TEM					955:957	TEM	955:957	TEM	955:957	The synthesized GQD by using ionic liquid were evaluated by spectroscopic (DLS, PL, XRD and Raman spectroscopy) and Transmission electron microscopic analysis (TEM).
31279287	3	79	theme	microscopic	933:943	arg1	analysis					945:952	spectroscopic (DLS, PL, XRD and Raman spectroscopy) and Transmission electron microscopic analysis	855:952	spectroscopic (DLS, PL, XRD and Raman spectroscopy) and Transmission electron microscopic analysis (TEM)	855:958	The synthesized GQD by using ionic liquid were evaluated by spectroscopic (DLS, PL, XRD and Raman spectroscopy) and Transmission electron microscopic analysis (TEM).
31279287	1	80	theme	quantum	303:309	arg1	GQDs					317:320	GQDs	317:320	GQDs	317:320	A novel nano-formulations of biocompatible, biodegradable and thermo-responsive graphene quantum dots (GQDs) loaded dextran/poly(N-isopropylacrylamide) (Dex/PNIPAM) copolymeric matrix was synthesized and analyzed the materials characterization, sustained drug delivery system, tissue feasibility in the tissue implantation site.
31279287	1	80	theme	quantum	303:309	arg1	dots					311:314	biocompatible, biodegradable and thermo-responsive graphene quantum dots	243:314	biocompatible, biodegradable and thermo-responsive graphene quantum dots (GQDs) loaded dextran/poly	243:341	A novel nano-formulations of biocompatible, biodegradable and thermo-responsive graphene quantum dots (GQDs) loaded dextran/poly(N-isopropylacrylamide) (Dex/PNIPAM) copolymeric matrix was synthesized and analyzed the materials characterization, sustained drug delivery system, tissue feasibility in the tissue implantation site.
31806967	10	0	theme	cytokines	1939:1947	arg1	score					1881:1885	arthritis index score	1865:1885	arthritis index score	1865:1885	Animal experiments confirmed that subcutaneous injection of NanoIGUR-loaded hydrogel (10 mg/kg every 3 days) and oral raw iguratimod (10mg/kg daily) showed similar efficacy in decreasing arthritis index score, pathological score, and expression of inflammatory cytokines.
31806967	10	0	theme	cytokines	1939:1947	arg1	score					1901:1905	pathological score	1888:1905	pathological score	1888:1905	Animal experiments confirmed that subcutaneous injection of NanoIGUR-loaded hydrogel (10 mg/kg every 3 days) and oral raw iguratimod (10mg/kg daily) showed similar efficacy in decreasing arthritis index score, pathological score, and expression of inflammatory cytokines.
31806967	10	0	theme	cytokines	1939:1947	arg1	expression					1912:1921	expression	1912:1921	expression of inflammatory cytokines	1912:1947	Animal experiments confirmed that subcutaneous injection of NanoIGUR-loaded hydrogel (10 mg/kg every 3 days) and oral raw iguratimod (10mg/kg daily) showed similar efficacy in decreasing arthritis index score, pathological score, and expression of inflammatory cytokines.
31806967	3	1	theme	precipitation	553:565	arg1	technology					573:582	liquid antisolvent precipitation (LAP) technology	534:582	liquid antisolvent precipitation (LAP) technology	534:582	METHODS IGUR was first encapsulated in biodegradable polyvinyl alcohol micelle by liquid antisolvent precipitation (LAP) technology, and then loaded into an in situ injectable hyaluronic acid hydrogel, which was cross-linked by PEG (Thiol)2 (HS-PEG-SH) through Michael addition reaction.
31806967	6	2	theme	average	1182:1188	arg1	size					1190:1193	an average size	1179:1193	an average size	1179:1193	RESULTS By the LAP technique, we acquired the amorphous form nanoiguratimod, with an average size of 458 nm, which had higher dissolution rates and higher stability.
31806967	2	3	theme	gastrointestinal	281:296	arg1	effects					303:309	gastrointestinal side effects	281:309	gastrointestinal side effects	281:309	To improve its bioavailability and to alleviate gastrointestinal side effects, we changed the formulation into nanoiguratimod-loaded hydrogel (NanoIGUR-loaded hydrogel) composites for sustained release of therapeutics.
31806967	3	4	theme	METHODS	452:458	arg1	IGUR					460:463	METHODS IGUR	452:463	METHODS IGUR	452:463	METHODS IGUR was first encapsulated in biodegradable polyvinyl alcohol micelle by liquid antisolvent precipitation (LAP) technology, and then loaded into an in situ injectable hyaluronic acid hydrogel, which was cross-linked by PEG (Thiol)2 (HS-PEG-SH) through Michael addition reaction.
31806967	3	5	theme	Michael	713:719	arg1	addition					721:728	Michael addition reaction	713:737	Michael addition reaction	713:737	METHODS IGUR was first encapsulated in biodegradable polyvinyl alcohol micelle by liquid antisolvent precipitation (LAP) technology, and then loaded into an in situ injectable hyaluronic acid hydrogel, which was cross-linked by PEG (Thiol)2 (HS-PEG-SH) through Michael addition reaction.
31806967	6	6	theme	dissolution	1223:1233	arg1	rates					1235:1239	higher dissolution rates	1216:1239	higher dissolution rates	1216:1239	RESULTS By the LAP technique, we acquired the amorphous form nanoiguratimod, with an average size of 458 nm, which had higher dissolution rates and higher stability.
31806967	6	7	theme	amorphous	1143:1151	arg1	nanoiguratimod					1158:1171	the amorphous form nanoiguratimod	1139:1171	the amorphous form nanoiguratimod	1139:1171	RESULTS By the LAP technique, we acquired the amorphous form nanoiguratimod, with an average size of 458 nm, which had higher dissolution rates and higher stability.
31806967	2	8	theme	hydrogel	366:373	arg1	composites					402:411	nanoiguratimod-loaded hydrogel (NanoIGUR-loaded hydrogel) composites	344:411	nanoiguratimod-loaded hydrogel (NanoIGUR-loaded hydrogel) composites	344:411	To improve its bioavailability and to alleviate gastrointestinal side effects, we changed the formulation into nanoiguratimod-loaded hydrogel (NanoIGUR-loaded hydrogel) composites for sustained release of therapeutics.
31806967	1	9	theme	rheumatoid	206:215	arg1	RA					228:229	RA	228:229	RA	228:229	BACKGROUND Iguratimod (IGUR) is a novel disease-modifying antirheumatic drug used for treating rheumatoid arthritis (RA).
31806967	1	9	theme	rheumatoid	206:215	arg1	arthritis					217:225	rheumatoid arthritis	206:225	rheumatoid arthritis (RA)	206:230	BACKGROUND Iguratimod (IGUR) is a novel disease-modifying antirheumatic drug used for treating rheumatoid arthritis (RA).
31806967	12	10	theme	drug-delivery	2119:2131	arg1	approach					2133:2140	a promising drug-delivery approach	2107:2140	a promising drug-delivery approach	2107:2140	It could be a promising drug-delivery approach in the management of RA.
31806967	12	10	theme	drug-delivery	2119:2131	arg1	It					2095:2096	It	2095:2096	It	2095:2096	It could be a promising drug-delivery approach in the management of RA.
31806967	8	11	theme	hydrogel	1431:1438	arg1	concentrations					1397:1410	Different concentrations	1387:1410	Different concentrations of NanoIGUR-loaded hydrogel	1387:1438	Different concentrations of NanoIGUR-loaded hydrogel inhibited the proliferation, migration, and invasion of RA-FLS.
31806967	6	12	theme	higher	1216:1221	arg1	rates					1235:1239	higher dissolution rates	1216:1239	higher dissolution rates	1216:1239	RESULTS By the LAP technique, we acquired the amorphous form nanoiguratimod, with an average size of 458 nm, which had higher dissolution rates and higher stability.
31806967	3	13	theme	hyaluronic	628:637	arg1	hydrogel					644:651	an in situ injectable hyaluronic acid hydrogel	606:651	an in situ injectable hyaluronic acid hydrogel	606:651	METHODS IGUR was first encapsulated in biodegradable polyvinyl alcohol micelle by liquid antisolvent precipitation (LAP) technology, and then loaded into an in situ injectable hyaluronic acid hydrogel, which was cross-linked by PEG (Thiol)2 (HS-PEG-SH) through Michael addition reaction.
31806967	5	14	theme	arthritis	1061:1069	arg1	rats					1077:1080	collagen-induced arthritis (CIA) rats	1044:1080	collagen-induced arthritis (CIA) rats	1044:1080	In vivo, the pharmacokinetics of NanoIGUR-loaded hydrogel was assessed and the efficacy of NanoIGUR-loaded hydrogel in treating collagen-induced arthritis (CIA) rats was evaluated.
31806967	11	15	theme	NanoIGUR-loaded	1990:2004	arg1	hydrogel					2006:2013	NanoIGUR-loaded hydrogel	1990:2013	NanoIGUR-loaded hydrogel	1990:2013	CONCLUSION Overall, we demonstrate that NanoIGUR-loaded hydrogel provides a new route of administration and extends the administration interval.
31806967	12	16	from	approach	2133:2140	arg1	management					2149:2158	the management	2145:2158	the management of RA	2145:2164	It could be a promising drug-delivery approach in the management of RA.
31806967	6	17	theme	nm	1202:1203	arg1	size					1190:1193	an average size	1179:1193	an average size	1179:1193	RESULTS By the LAP technique, we acquired the amorphous form nanoiguratimod, with an average size of 458 nm, which had higher dissolution rates and higher stability.
31806967	4	18	from	patients	891:898	arg1	RA-FLS					875:880	RA-FLS	875:880	RA-FLS	875:880	In vitro, the biological effects (proliferation, migration, and invasion) of NanoIGUR-loaded hydrogel on fibroblast-like synoviocytes (RA-FLS) from RA patients were evaluated.
31806967	4	18	from	patients	891:898	arg1	synoviocytes					861:872	fibroblast-like synoviocytes	845:872	fibroblast-like synoviocytes (RA-FLS) from RA patients	845:898	In vitro, the biological effects (proliferation, migration, and invasion) of NanoIGUR-loaded hydrogel on fibroblast-like synoviocytes (RA-FLS) from RA patients were evaluated.
31806967	10	19	theme	raw	1796:1798	arg1	iguratimod					1800:1809	oral raw iguratimod	1791:1809	oral raw iguratimod (10mg/kg daily)	1791:1825	Animal experiments confirmed that subcutaneous injection of NanoIGUR-loaded hydrogel (10 mg/kg every 3 days) and oral raw iguratimod (10mg/kg daily) showed similar efficacy in decreasing arthritis index score, pathological score, and expression of inflammatory cytokines.
31806967	0	20	theme	Nanoiguratimod	57:70	arg1	Encapsulation					40:52	Encapsulation	40:52	Encapsulation Of Nanoiguratimod	40:70	In Situ Forming Injectable Hydrogel For Encapsulation Of Nanoiguratimod And Sustained Release Of Therapeutics.
31806967	11	21	theme	new	2026:2028	arg1	route					2030:2034	a new route	2024:2034	a new route of administration	2024:2052	CONCLUSION Overall, we demonstrate that NanoIGUR-loaded hydrogel provides a new route of administration and extends the administration interval.
31806967	9	22	theme	pharmacokinetic	1508:1522	arg1	parameters					1524:1533	The pharmacokinetic parameters	1504:1533	The pharmacokinetic parameters	1504:1533	The pharmacokinetic parameters showed better bioavailability and longer half-life time with NanoIGUR-loaded hydrogel by subcutaneous administration than oral raw iguratimod.
31806967	12	23	theme	promising	2109:2117	arg1	approach					2133:2140	a promising drug-delivery approach	2107:2140	a promising drug-delivery approach	2107:2140	It could be a promising drug-delivery approach in the management of RA.
31806967	12	23	theme	promising	2109:2117	arg1	It					2095:2096	It	2095:2096	It	2095:2096	It could be a promising drug-delivery approach in the management of RA.
31806967	0	24	theme	Sustained	76:84	arg1	Release					86:92	Sustained Release	76:92	Sustained Release Of Therapeutics	76:108	In Situ Forming Injectable Hydrogel For Encapsulation Of Nanoiguratimod And Sustained Release Of Therapeutics.
31806967	1	25	theme	disease-modifying	151:167	arg1	Iguratimod					122:131	BACKGROUND Iguratimod	111:131	BACKGROUND Iguratimod (IGUR)	111:138	BACKGROUND Iguratimod (IGUR) is a novel disease-modifying antirheumatic drug used for treating rheumatoid arthritis (RA).
31806967	1	25	theme	disease-modifying	151:167	arg1	drug					183:186	a novel disease-modifying antirheumatic drug	143:186	a novel disease-modifying antirheumatic drug used for treating rheumatoid arthritis (RA)	143:230	BACKGROUND Iguratimod (IGUR) is a novel disease-modifying antirheumatic drug used for treating rheumatoid arthritis (RA).
31806967	8	26	theme	RA-FLS	1496:1501	arg1	migration					1469:1477	migration	1469:1477	migration	1469:1477	Different concentrations of NanoIGUR-loaded hydrogel inhibited the proliferation, migration, and invasion of RA-FLS.
31806967	8	26	theme	RA-FLS	1496:1501	arg1	invasion					1484:1491	invasion	1484:1491	invasion	1484:1491	Different concentrations of NanoIGUR-loaded hydrogel inhibited the proliferation, migration, and invasion of RA-FLS.
31806967	8	26	theme	RA-FLS	1496:1501	arg1	proliferation					1454:1466	proliferation	1454:1466	proliferation	1454:1466	Different concentrations of NanoIGUR-loaded hydrogel inhibited the proliferation, migration, and invasion of RA-FLS.
31806967	9	27	theme	NanoIGUR-loaded	1596:1610	arg1	hydrogel					1612:1619	NanoIGUR-loaded hydrogel	1596:1619	NanoIGUR-loaded hydrogel by subcutaneous administration	1596:1650	The pharmacokinetic parameters showed better bioavailability and longer half-life time with NanoIGUR-loaded hydrogel by subcutaneous administration than oral raw iguratimod.
31806967	10	28	theme	oral	1791:1794	arg1	iguratimod					1800:1809	oral raw iguratimod	1791:1809	oral raw iguratimod (10mg/kg daily)	1791:1825	Animal experiments confirmed that subcutaneous injection of NanoIGUR-loaded hydrogel (10 mg/kg every 3 days) and oral raw iguratimod (10mg/kg daily) showed similar efficacy in decreasing arthritis index score, pathological score, and expression of inflammatory cytokines.
31806967	4	29	from	effects	765:771	arg1	RA-FLS					875:880	RA-FLS	875:880	RA-FLS	875:880	In vitro, the biological effects (proliferation, migration, and invasion) of NanoIGUR-loaded hydrogel on fibroblast-like synoviocytes (RA-FLS) from RA patients were evaluated.
31806967	4	29	from	effects	765:771	arg1	synoviocytes					861:872	fibroblast-like synoviocytes	845:872	fibroblast-like synoviocytes (RA-FLS) from RA patients	845:898	In vitro, the biological effects (proliferation, migration, and invasion) of NanoIGUR-loaded hydrogel on fibroblast-like synoviocytes (RA-FLS) from RA patients were evaluated.
31806967	3	30	theme	polyvinyl	505:513	arg1	micelle					523:529	biodegradable polyvinyl alcohol micelle	491:529	biodegradable polyvinyl alcohol micelle	491:529	METHODS IGUR was first encapsulated in biodegradable polyvinyl alcohol micelle by liquid antisolvent precipitation (LAP) technology, and then loaded into an in situ injectable hyaluronic acid hydrogel, which was cross-linked by PEG (Thiol)2 (HS-PEG-SH) through Michael addition reaction.
31806967	2	31	theme	sustained	417:425	arg1	release					427:433	sustained release	417:433	sustained release of therapeutics	417:449	To improve its bioavailability and to alleviate gastrointestinal side effects, we changed the formulation into nanoiguratimod-loaded hydrogel (NanoIGUR-loaded hydrogel) composites for sustained release of therapeutics.
31806967	9	32	theme	longer	1569:1574	arg1	time					1586:1589	longer half-life time	1569:1589	longer half-life time	1569:1589	The pharmacokinetic parameters showed better bioavailability and longer half-life time with NanoIGUR-loaded hydrogel by subcutaneous administration than oral raw iguratimod.
31806967	3	33	theme	LAP	568:570	arg1	technology					573:582	liquid antisolvent precipitation (LAP) technology	534:582	liquid antisolvent precipitation (LAP) technology	534:582	METHODS IGUR was first encapsulated in biodegradable polyvinyl alcohol micelle by liquid antisolvent precipitation (LAP) technology, and then loaded into an in situ injectable hyaluronic acid hydrogel, which was cross-linked by PEG (Thiol)2 (HS-PEG-SH) through Michael addition reaction.
31806967	10	34	dep	hydrogel	1754:1761	arg1	mg/kg					1767:1771	10 mg/kg	1764:1771	10 mg/kg every 3 days	1764:1784	Animal experiments confirmed that subcutaneous injection of NanoIGUR-loaded hydrogel (10 mg/kg every 3 days) and oral raw iguratimod (10mg/kg daily) showed similar efficacy in decreasing arthritis index score, pathological score, and expression of inflammatory cytokines.
31806967	10	35	theme	Animal	1678:1683	arg1	experiments					1685:1695	Animal experiments	1678:1695	Animal experiments	1678:1695	Animal experiments confirmed that subcutaneous injection of NanoIGUR-loaded hydrogel (10 mg/kg every 3 days) and oral raw iguratimod (10mg/kg daily) showed similar efficacy in decreasing arthritis index score, pathological score, and expression of inflammatory cytokines.
31806967	3	36	theme	PEG	680:682	arg1	Thiol					685:689	PEG (Thiol)2	680:691	PEG (Thiol)2 (HS-PEG-SH) through Michael addition reaction	680:737	METHODS IGUR was first encapsulated in biodegradable polyvinyl alcohol micelle by liquid antisolvent precipitation (LAP) technology, and then loaded into an in situ injectable hyaluronic acid hydrogel, which was cross-linked by PEG (Thiol)2 (HS-PEG-SH) through Michael addition reaction.
31806967	3	36	theme	PEG	680:682	arg1	HS-PEG-SH					694:702	HS-PEG-SH	694:702	HS-PEG-SH	694:702	METHODS IGUR was first encapsulated in biodegradable polyvinyl alcohol micelle by liquid antisolvent precipitation (LAP) technology, and then loaded into an in situ injectable hyaluronic acid hydrogel, which was cross-linked by PEG (Thiol)2 (HS-PEG-SH) through Michael addition reaction.
31806967	3	37	theme	biodegradable	491:503	arg1	micelle					523:529	biodegradable polyvinyl alcohol micelle	491:529	biodegradable polyvinyl alcohol micelle	491:529	METHODS IGUR was first encapsulated in biodegradable polyvinyl alcohol micelle by liquid antisolvent precipitation (LAP) technology, and then loaded into an in situ injectable hyaluronic acid hydrogel, which was cross-linked by PEG (Thiol)2 (HS-PEG-SH) through Michael addition reaction.
31806967	5	38	theme	NanoIGUR-loaded	949:963	arg1	hydrogel					965:972	NanoIGUR-loaded hydrogel	949:972	NanoIGUR-loaded hydrogel	949:972	In vivo, the pharmacokinetics of NanoIGUR-loaded hydrogel was assessed and the efficacy of NanoIGUR-loaded hydrogel in treating collagen-induced arthritis (CIA) rats was evaluated.
31806967	7	39	theme	hydrogel	1288:1295	arg1	composite					1297:1305	hydrogel composite	1288:1305	hydrogel composite in PBS	1288:1312	The release of IGUR from hydrogel composite in PBS was gradual and sustained for up to 72 hrs compared with nanoiguratimod.
31806967	10	40	theme	pathological	1888:1899	arg1	score					1901:1905	pathological score	1888:1905	pathological score	1888:1905	Animal experiments confirmed that subcutaneous injection of NanoIGUR-loaded hydrogel (10 mg/kg every 3 days) and oral raw iguratimod (10mg/kg daily) showed similar efficacy in decreasing arthritis index score, pathological score, and expression of inflammatory cytokines.
31806967	10	41	theme	subcutaneous	1712:1723	arg1	injection					1725:1733	subcutaneous injection	1712:1733	subcutaneous injection of NanoIGUR-loaded hydrogel (10 mg/kg every 3 days) and oral raw iguratimod (10mg/kg daily)	1712:1825	Animal experiments confirmed that subcutaneous injection of NanoIGUR-loaded hydrogel (10 mg/kg every 3 days) and oral raw iguratimod (10mg/kg daily) showed similar efficacy in decreasing arthritis index score, pathological score, and expression of inflammatory cytokines.
31806967	3	42	dep	in	609:610	arg1	situ					612:615	situ	612:615	situ	612:615	METHODS IGUR was first encapsulated in biodegradable polyvinyl alcohol micelle by liquid antisolvent precipitation (LAP) technology, and then loaded into an in situ injectable hyaluronic acid hydrogel, which was cross-linked by PEG (Thiol)2 (HS-PEG-SH) through Michael addition reaction.
31806967	7	43	theme	IGUR	1278:1281	arg1	gradual					1318:1324	gradual	1318:1324	gradual	1318:1324	The release of IGUR from hydrogel composite in PBS was gradual and sustained for up to 72 hrs compared with nanoiguratimod.
31806967	7	43	theme	IGUR	1278:1281	arg1	release					1267:1273	The release	1263:1273	The release of IGUR from hydrogel composite in PBS	1263:1312	The release of IGUR from hydrogel composite in PBS was gradual and sustained for up to 72 hrs compared with nanoiguratimod.
31806967	4	44	theme	NanoIGUR-loaded	817:831	arg1	hydrogel					833:840	NanoIGUR-loaded hydrogel	817:840	NanoIGUR-loaded hydrogel	817:840	In vitro, the biological effects (proliferation, migration, and invasion) of NanoIGUR-loaded hydrogel on fibroblast-like synoviocytes (RA-FLS) from RA patients were evaluated.
31806967	2	45	theme	nanoiguratimod-loaded	344:364	arg1	hydrogel					366:373	nanoiguratimod-loaded hydrogel	344:373	nanoiguratimod-loaded hydrogel (NanoIGUR-loaded hydrogel) composites	344:411	To improve its bioavailability and to alleviate gastrointestinal side effects, we changed the formulation into nanoiguratimod-loaded hydrogel (NanoIGUR-loaded hydrogel) composites for sustained release of therapeutics.
31806967	2	45	theme	nanoiguratimod-loaded	344:364	arg1	hydrogel					392:399	NanoIGUR-loaded hydrogel	376:399	NanoIGUR-loaded hydrogel	376:399	To improve its bioavailability and to alleviate gastrointestinal side effects, we changed the formulation into nanoiguratimod-loaded hydrogel (NanoIGUR-loaded hydrogel) composites for sustained release of therapeutics.
31806967	5	46	theme	collagen-induced	1044:1059	arg1	CIA					1072:1074	CIA	1072:1074	CIA	1072:1074	In vivo, the pharmacokinetics of NanoIGUR-loaded hydrogel was assessed and the efficacy of NanoIGUR-loaded hydrogel in treating collagen-induced arthritis (CIA) rats was evaluated.
31806967	5	46	theme	collagen-induced	1044:1059	arg1	arthritis					1061:1069	collagen-induced arthritis	1044:1069	collagen-induced arthritis (CIA) rats	1044:1080	In vivo, the pharmacokinetics of NanoIGUR-loaded hydrogel was assessed and the efficacy of NanoIGUR-loaded hydrogel in treating collagen-induced arthritis (CIA) rats was evaluated.
31806967	3	47	theme	alcohol	515:521	arg1	micelle					523:529	biodegradable polyvinyl alcohol micelle	491:529	biodegradable polyvinyl alcohol micelle	491:529	METHODS IGUR was first encapsulated in biodegradable polyvinyl alcohol micelle by liquid antisolvent precipitation (LAP) technology, and then loaded into an in situ injectable hyaluronic acid hydrogel, which was cross-linked by PEG (Thiol)2 (HS-PEG-SH) through Michael addition reaction.
31806967	9	48	theme	oral	1657:1660	arg1	iguratimod					1666:1675	oral raw iguratimod	1657:1675	oral raw iguratimod	1657:1675	The pharmacokinetic parameters showed better bioavailability and longer half-life time with NanoIGUR-loaded hydrogel by subcutaneous administration than oral raw iguratimod.
31806967	7	49	from	composite	1297:1305	arg1	gradual					1318:1324	gradual	1318:1324	gradual	1318:1324	The release of IGUR from hydrogel composite in PBS was gradual and sustained for up to 72 hrs compared with nanoiguratimod.
31806967	7	49	from	composite	1297:1305	arg1	PBS					1310:1312	PBS	1310:1312	PBS	1310:1312	The release of IGUR from hydrogel composite in PBS was gradual and sustained for up to 72 hrs compared with nanoiguratimod.
31806967	7	49	from	composite	1297:1305	arg1	release					1267:1273	The release	1263:1273	The release of IGUR from hydrogel composite in PBS	1263:1312	The release of IGUR from hydrogel composite in PBS was gradual and sustained for up to 72 hrs compared with nanoiguratimod.
31806967	8	50	theme	NanoIGUR-loaded	1415:1429	arg1	hydrogel					1431:1438	NanoIGUR-loaded hydrogel	1415:1438	NanoIGUR-loaded hydrogel	1415:1438	Different concentrations of NanoIGUR-loaded hydrogel inhibited the proliferation, migration, and invasion of RA-FLS.
31806967	5	51	theme	NanoIGUR-loaded	1007:1021	arg1	hydrogel					1023:1030	NanoIGUR-loaded hydrogel	1007:1030	NanoIGUR-loaded hydrogel	1007:1030	In vivo, the pharmacokinetics of NanoIGUR-loaded hydrogel was assessed and the efficacy of NanoIGUR-loaded hydrogel in treating collagen-induced arthritis (CIA) rats was evaluated.
31806967	10	52	theme	arthritis	1865:1873	arg1	score					1881:1885	arthritis index score	1865:1885	arthritis index score	1865:1885	Animal experiments confirmed that subcutaneous injection of NanoIGUR-loaded hydrogel (10 mg/kg every 3 days) and oral raw iguratimod (10mg/kg daily) showed similar efficacy in decreasing arthritis index score, pathological score, and expression of inflammatory cytokines.
31806967	3	53	theme	antisolvent	541:551	arg1	technology					573:582	liquid antisolvent precipitation (LAP) technology	534:582	liquid antisolvent precipitation (LAP) technology	534:582	METHODS IGUR was first encapsulated in biodegradable polyvinyl alcohol micelle by liquid antisolvent precipitation (LAP) technology, and then loaded into an in situ injectable hyaluronic acid hydrogel, which was cross-linked by PEG (Thiol)2 (HS-PEG-SH) through Michael addition reaction.
31806967	10	54	theme	inflammatory	1926:1937	arg1	cytokines					1939:1947	inflammatory cytokines	1926:1947	inflammatory cytokines	1926:1947	Animal experiments confirmed that subcutaneous injection of NanoIGUR-loaded hydrogel (10 mg/kg every 3 days) and oral raw iguratimod (10mg/kg daily) showed similar efficacy in decreasing arthritis index score, pathological score, and expression of inflammatory cytokines.
31806967	2	55	theme	side	298:301	arg1	effects					303:309	gastrointestinal side effects	281:309	gastrointestinal side effects	281:309	To improve its bioavailability and to alleviate gastrointestinal side effects, we changed the formulation into nanoiguratimod-loaded hydrogel (NanoIGUR-loaded hydrogel) composites for sustained release of therapeutics.
31806967	4	56	dep	effects	765:771	arg1	effects					765:771	the biological effects	750:771	the biological effects (proliferation, migration, and invasion) of NanoIGUR-loaded hydrogel on fibroblast-like synoviocytes (RA-FLS) from RA patients	750:898	In vitro, the biological effects (proliferation, migration, and invasion) of NanoIGUR-loaded hydrogel on fibroblast-like synoviocytes (RA-FLS) from RA patients were evaluated.
31806967	4	56	dep	effects	765:771	arg1	migration					789:797	migration	789:797	migration	789:797	In vitro, the biological effects (proliferation, migration, and invasion) of NanoIGUR-loaded hydrogel on fibroblast-like synoviocytes (RA-FLS) from RA patients were evaluated.
31806967	4	56	dep	effects	765:771	arg1	invasion					804:811	invasion	804:811	invasion	804:811	In vitro, the biological effects (proliferation, migration, and invasion) of NanoIGUR-loaded hydrogel on fibroblast-like synoviocytes (RA-FLS) from RA patients were evaluated.
31806967	4	56	dep	effects	765:771	arg1	proliferation					774:786	proliferation	774:786	proliferation	774:786	In vitro, the biological effects (proliferation, migration, and invasion) of NanoIGUR-loaded hydrogel on fibroblast-like synoviocytes (RA-FLS) from RA patients were evaluated.
31806967	6	57	theme	higher	1245:1250	arg1	stability					1252:1260	higher stability	1245:1260	higher stability	1245:1260	RESULTS By the LAP technique, we acquired the amorphous form nanoiguratimod, with an average size of 458 nm, which had higher dissolution rates and higher stability.
31806967	11	58	theme	administration	2039:2052	arg1	route					2030:2034	a new route	2024:2034	a new route of administration	2024:2052	CONCLUSION Overall, we demonstrate that NanoIGUR-loaded hydrogel provides a new route of administration and extends the administration interval.
31806967	1	59	theme	antirheumatic	169:181	arg1	Iguratimod					122:131	BACKGROUND Iguratimod	111:131	BACKGROUND Iguratimod (IGUR)	111:138	BACKGROUND Iguratimod (IGUR) is a novel disease-modifying antirheumatic drug used for treating rheumatoid arthritis (RA).
31806967	1	59	theme	antirheumatic	169:181	arg1	drug					183:186	a novel disease-modifying antirheumatic drug	143:186	a novel disease-modifying antirheumatic drug used for treating rheumatoid arthritis (RA)	143:230	BACKGROUND Iguratimod (IGUR) is a novel disease-modifying antirheumatic drug used for treating rheumatoid arthritis (RA).
31806967	4	60	theme	hydrogel	833:840	arg1	effects					765:771	the biological effects	750:771	the biological effects (proliferation, migration, and invasion) of NanoIGUR-loaded hydrogel on fibroblast-like synoviocytes (RA-FLS) from RA patients	750:898	In vitro, the biological effects (proliferation, migration, and invasion) of NanoIGUR-loaded hydrogel on fibroblast-like synoviocytes (RA-FLS) from RA patients were evaluated.
31806967	4	60	theme	hydrogel	833:840	arg1	migration					789:797	migration	789:797	migration	789:797	In vitro, the biological effects (proliferation, migration, and invasion) of NanoIGUR-loaded hydrogel on fibroblast-like synoviocytes (RA-FLS) from RA patients were evaluated.
31806967	4	60	theme	hydrogel	833:840	arg1	invasion					804:811	invasion	804:811	invasion	804:811	In vitro, the biological effects (proliferation, migration, and invasion) of NanoIGUR-loaded hydrogel on fibroblast-like synoviocytes (RA-FLS) from RA patients were evaluated.
31806967	4	60	theme	hydrogel	833:840	arg1	proliferation					774:786	proliferation	774:786	proliferation	774:786	In vitro, the biological effects (proliferation, migration, and invasion) of NanoIGUR-loaded hydrogel on fibroblast-like synoviocytes (RA-FLS) from RA patients were evaluated.
31806967	7	61	dep	72	1350:1351	arg1	to					1347:1348	to	1347:1348	to	1347:1348	The release of IGUR from hydrogel composite in PBS was gradual and sustained for up to 72 hrs compared with nanoiguratimod.
31806967	4	62	theme	fibroblast-like	845:859	arg1	RA-FLS					875:880	RA-FLS	875:880	RA-FLS	875:880	In vitro, the biological effects (proliferation, migration, and invasion) of NanoIGUR-loaded hydrogel on fibroblast-like synoviocytes (RA-FLS) from RA patients were evaluated.
31806967	4	62	theme	fibroblast-like	845:859	arg1	synoviocytes					861:872	fibroblast-like synoviocytes	845:872	fibroblast-like synoviocytes (RA-FLS) from RA patients	845:898	In vitro, the biological effects (proliferation, migration, and invasion) of NanoIGUR-loaded hydrogel on fibroblast-like synoviocytes (RA-FLS) from RA patients were evaluated.
31806967	6	63	theme	form	1153:1156	arg1	nanoiguratimod					1158:1171	the amorphous form nanoiguratimod	1139:1171	the amorphous form nanoiguratimod	1139:1171	RESULTS By the LAP technique, we acquired the amorphous form nanoiguratimod, with an average size of 458 nm, which had higher dissolution rates and higher stability.
31806967	11	64	dep	CONCLUSION	1950:1959	arg1	demonstrate					1973:1983	demonstrate	1973:1983	demonstrate that NanoIGUR-loaded hydrogel provides a new route of administration and extends the administration interval	1973:2092	CONCLUSION Overall, we demonstrate that NanoIGUR-loaded hydrogel provides a new route of administration and extends the administration interval.
31806967	11	65	theme	administration	2070:2083	arg1	interval					2085:2092	the administration interval	2066:2092	the administration interval	2066:2092	CONCLUSION Overall, we demonstrate that NanoIGUR-loaded hydrogel provides a new route of administration and extends the administration interval.
31806967	3	66	theme	injectable	617:626	arg1	hydrogel					644:651	an in situ injectable hyaluronic acid hydrogel	606:651	an in situ injectable hyaluronic acid hydrogel	606:651	METHODS IGUR was first encapsulated in biodegradable polyvinyl alcohol micelle by liquid antisolvent precipitation (LAP) technology, and then loaded into an in situ injectable hyaluronic acid hydrogel, which was cross-linked by PEG (Thiol)2 (HS-PEG-SH) through Michael addition reaction.
31806967	12	67	theme	RA	2163:2164	arg1	management					2149:2158	the management	2145:2158	the management of RA	2145:2164	It could be a promising drug-delivery approach in the management of RA.
31806967	3	68	theme	in	609:610	arg1	hydrogel					644:651	an in situ injectable hyaluronic acid hydrogel	606:651	an in situ injectable hyaluronic acid hydrogel	606:651	METHODS IGUR was first encapsulated in biodegradable polyvinyl alcohol micelle by liquid antisolvent precipitation (LAP) technology, and then loaded into an in situ injectable hyaluronic acid hydrogel, which was cross-linked by PEG (Thiol)2 (HS-PEG-SH) through Michael addition reaction.
31806967	4	69	theme	RA	888:889	arg1	patients					891:898	RA patients	888:898	RA patients	888:898	In vitro, the biological effects (proliferation, migration, and invasion) of NanoIGUR-loaded hydrogel on fibroblast-like synoviocytes (RA-FLS) from RA patients were evaluated.
31806967	10	70	dep	iguratimod	1800:1809	arg1	10mg/kg					1812:1818	10mg/kg	1812:1818	10mg/kg daily	1812:1824	Animal experiments confirmed that subcutaneous injection of NanoIGUR-loaded hydrogel (10 mg/kg every 3 days) and oral raw iguratimod (10mg/kg daily) showed similar efficacy in decreasing arthritis index score, pathological score, and expression of inflammatory cytokines.
31806967	10	71	theme	index	1875:1879	arg1	score					1881:1885	arthritis index score	1865:1885	arthritis index score	1865:1885	Animal experiments confirmed that subcutaneous injection of NanoIGUR-loaded hydrogel (10 mg/kg every 3 days) and oral raw iguratimod (10mg/kg daily) showed similar efficacy in decreasing arthritis index score, pathological score, and expression of inflammatory cytokines.
31806967	5	72	theme	hydrogel	965:972	arg1	pharmacokinetics					929:944	the pharmacokinetics	925:944	the pharmacokinetics of NanoIGUR-loaded hydrogel	925:972	In vivo, the pharmacokinetics of NanoIGUR-loaded hydrogel was assessed and the efficacy of NanoIGUR-loaded hydrogel in treating collagen-induced arthritis (CIA) rats was evaluated.
31806967	10	73	theme	hydrogel	1754:1761	arg1	injection					1725:1733	subcutaneous injection	1712:1733	subcutaneous injection of NanoIGUR-loaded hydrogel (10 mg/kg every 3 days) and oral raw iguratimod (10mg/kg daily)	1712:1825	Animal experiments confirmed that subcutaneous injection of NanoIGUR-loaded hydrogel (10 mg/kg every 3 days) and oral raw iguratimod (10mg/kg daily) showed similar efficacy in decreasing arthritis index score, pathological score, and expression of inflammatory cytokines.
31806967	3	74	theme	acid	639:642	arg1	hydrogel					644:651	an in situ injectable hyaluronic acid hydrogel	606:651	an in situ injectable hyaluronic acid hydrogel	606:651	METHODS IGUR was first encapsulated in biodegradable polyvinyl alcohol micelle by liquid antisolvent precipitation (LAP) technology, and then loaded into an in situ injectable hyaluronic acid hydrogel, which was cross-linked by PEG (Thiol)2 (HS-PEG-SH) through Michael addition reaction.
31806967	1	75	theme	novel	145:149	arg1	Iguratimod					122:131	BACKGROUND Iguratimod	111:131	BACKGROUND Iguratimod (IGUR)	111:138	BACKGROUND Iguratimod (IGUR) is a novel disease-modifying antirheumatic drug used for treating rheumatoid arthritis (RA).
31806967	1	75	theme	novel	145:149	arg1	drug					183:186	a novel disease-modifying antirheumatic drug	143:186	a novel disease-modifying antirheumatic drug used for treating rheumatoid arthritis (RA)	143:230	BACKGROUND Iguratimod (IGUR) is a novel disease-modifying antirheumatic drug used for treating rheumatoid arthritis (RA).
31806967	0	76	theme	Therapeutics	97:108	arg1	Release					86:92	Sustained Release	76:92	Sustained Release Of Therapeutics	76:108	In Situ Forming Injectable Hydrogel For Encapsulation Of Nanoiguratimod And Sustained Release Of Therapeutics.
31806967	0	76	theme	Therapeutics	97:108	arg1	Hydrogel					27:34	Injectable Hydrogel	16:34	Injectable Hydrogel For Encapsulation Of Nanoiguratimod	16:70	In Situ Forming Injectable Hydrogel For Encapsulation Of Nanoiguratimod And Sustained Release Of Therapeutics.
31806967	9	77	theme	better	1542:1547	arg1	bioavailability					1549:1563	better bioavailability	1542:1563	better bioavailability	1542:1563	The pharmacokinetic parameters showed better bioavailability and longer half-life time with NanoIGUR-loaded hydrogel by subcutaneous administration than oral raw iguratimod.
31806967	10	78	theme	iguratimod	1800:1809	arg1	injection					1725:1733	subcutaneous injection	1712:1733	subcutaneous injection of NanoIGUR-loaded hydrogel (10 mg/kg every 3 days) and oral raw iguratimod (10mg/kg daily)	1712:1825	Animal experiments confirmed that subcutaneous injection of NanoIGUR-loaded hydrogel (10 mg/kg every 3 days) and oral raw iguratimod (10mg/kg daily) showed similar efficacy in decreasing arthritis index score, pathological score, and expression of inflammatory cytokines.
31806967	10	79	dep	mg/kg	1767:1771	arg1	days					1781:1784	every 3 days	1773:1784	10 mg/kg every 3 days	1764:1784	Animal experiments confirmed that subcutaneous injection of NanoIGUR-loaded hydrogel (10 mg/kg every 3 days) and oral raw iguratimod (10mg/kg daily) showed similar efficacy in decreasing arthritis index score, pathological score, and expression of inflammatory cytokines.
31806967	4	80	theme	biological	754:763	arg1	effects					765:771	the biological effects	750:771	the biological effects (proliferation, migration, and invasion) of NanoIGUR-loaded hydrogel on fibroblast-like synoviocytes (RA-FLS) from RA patients	750:898	In vitro, the biological effects (proliferation, migration, and invasion) of NanoIGUR-loaded hydrogel on fibroblast-like synoviocytes (RA-FLS) from RA patients were evaluated.
31806967	4	80	theme	biological	754:763	arg1	migration					789:797	migration	789:797	migration	789:797	In vitro, the biological effects (proliferation, migration, and invasion) of NanoIGUR-loaded hydrogel on fibroblast-like synoviocytes (RA-FLS) from RA patients were evaluated.
31806967	4	80	theme	biological	754:763	arg1	invasion					804:811	invasion	804:811	invasion	804:811	In vitro, the biological effects (proliferation, migration, and invasion) of NanoIGUR-loaded hydrogel on fibroblast-like synoviocytes (RA-FLS) from RA patients were evaluated.
31806967	4	80	theme	biological	754:763	arg1	proliferation					774:786	proliferation	774:786	proliferation	774:786	In vitro, the biological effects (proliferation, migration, and invasion) of NanoIGUR-loaded hydrogel on fibroblast-like synoviocytes (RA-FLS) from RA patients were evaluated.
31806967	9	81	theme	half-life	1576:1584	arg1	time					1586:1589	longer half-life time	1569:1589	longer half-life time	1569:1589	The pharmacokinetic parameters showed better bioavailability and longer half-life time with NanoIGUR-loaded hydrogel by subcutaneous administration than oral raw iguratimod.
31806967	0	82	theme	Injectable	16:25	arg1	Hydrogel					27:34	Injectable Hydrogel	16:34	Injectable Hydrogel For Encapsulation Of Nanoiguratimod	16:70	In Situ Forming Injectable Hydrogel For Encapsulation Of Nanoiguratimod And Sustained Release Of Therapeutics.
31806967	6	83	contain	had	1212:1214	arg1	size					1190:1193	an average size	1179:1193	an average size	1179:1193	RESULTS By the LAP technique, we acquired the amorphous form nanoiguratimod, with an average size of 458 nm, which had higher dissolution rates and higher stability.
31806967	6	83	contain	had	1212:1214	arg2	stability					1252:1260	higher stability	1245:1260	higher stability	1245:1260	RESULTS By the LAP technique, we acquired the amorphous form nanoiguratimod, with an average size of 458 nm, which had higher dissolution rates and higher stability.
31806967	6	83	contain	had	1212:1214	arg2	rates					1235:1239	higher dissolution rates	1216:1239	higher dissolution rates	1216:1239	RESULTS By the LAP technique, we acquired the amorphous form nanoiguratimod, with an average size of 458 nm, which had higher dissolution rates and higher stability.
31806967	2	84	theme	therapeutics	438:449	arg1	release					427:433	sustained release	417:433	sustained release of therapeutics	417:449	To improve its bioavailability and to alleviate gastrointestinal side effects, we changed the formulation into nanoiguratimod-loaded hydrogel (NanoIGUR-loaded hydrogel) composites for sustained release of therapeutics.
31806967	5	85	theme	hydrogel	1023:1030	arg1	efficacy					995:1002	the efficacy	991:1002	the efficacy of NanoIGUR-loaded hydrogel in treating collagen-induced arthritis (CIA) rats	991:1080	In vivo, the pharmacokinetics of NanoIGUR-loaded hydrogel was assessed and the efficacy of NanoIGUR-loaded hydrogel in treating collagen-induced arthritis (CIA) rats was evaluated.
31806967	6	86	dep	RESULTS	1097:1103	arg1	acquired					1130:1137	acquired	1130:1137	acquired	1130:1137	RESULTS By the LAP technique, we acquired the amorphous form nanoiguratimod, with an average size of 458 nm, which had higher dissolution rates and higher stability.
31806967	3	87	dep	addition	721:728	arg1	reaction					730:737	reaction	730:737	Michael addition reaction	713:737	METHODS IGUR was first encapsulated in biodegradable polyvinyl alcohol micelle by liquid antisolvent precipitation (LAP) technology, and then loaded into an in situ injectable hyaluronic acid hydrogel, which was cross-linked by PEG (Thiol)2 (HS-PEG-SH) through Michael addition reaction.
31806967	9	88	theme	subcutaneous	1624:1635	arg1	administration					1637:1650	subcutaneous administration	1624:1650	subcutaneous administration	1624:1650	The pharmacokinetic parameters showed better bioavailability and longer half-life time with NanoIGUR-loaded hydrogel by subcutaneous administration than oral raw iguratimod.
31806967	3	89	theme	liquid	534:539	arg1	technology					573:582	liquid antisolvent precipitation (LAP) technology	534:582	liquid antisolvent precipitation (LAP) technology	534:582	METHODS IGUR was first encapsulated in biodegradable polyvinyl alcohol micelle by liquid antisolvent precipitation (LAP) technology, and then loaded into an in situ injectable hyaluronic acid hydrogel, which was cross-linked by PEG (Thiol)2 (HS-PEG-SH) through Michael addition reaction.
31806967	1	90	theme	BACKGROUND	111:120	arg1	IGUR					134:137	IGUR	134:137	IGUR	134:137	BACKGROUND Iguratimod (IGUR) is a novel disease-modifying antirheumatic drug used for treating rheumatoid arthritis (RA).
31806967	1	90	theme	BACKGROUND	111:120	arg1	Iguratimod					122:131	BACKGROUND Iguratimod	111:131	BACKGROUND Iguratimod (IGUR)	111:138	BACKGROUND Iguratimod (IGUR) is a novel disease-modifying antirheumatic drug used for treating rheumatoid arthritis (RA).
31806967	1	90	theme	BACKGROUND	111:120	arg1	drug					183:186	a novel disease-modifying antirheumatic drug	143:186	a novel disease-modifying antirheumatic drug used for treating rheumatoid arthritis (RA)	143:230	BACKGROUND Iguratimod (IGUR) is a novel disease-modifying antirheumatic drug used for treating rheumatoid arthritis (RA).
31806967	10	91	theme	similar	1834:1840	arg1	efficacy					1842:1849	similar efficacy	1834:1849	similar efficacy	1834:1849	Animal experiments confirmed that subcutaneous injection of NanoIGUR-loaded hydrogel (10 mg/kg every 3 days) and oral raw iguratimod (10mg/kg daily) showed similar efficacy in decreasing arthritis index score, pathological score, and expression of inflammatory cytokines.
31806967	8	92	theme	Different	1387:1395	arg1	concentrations					1397:1410	Different concentrations	1387:1410	Different concentrations of NanoIGUR-loaded hydrogel	1387:1438	Different concentrations of NanoIGUR-loaded hydrogel inhibited the proliferation, migration, and invasion of RA-FLS.
31806967	10	93	theme	NanoIGUR-loaded	1738:1752	arg1	hydrogel					1754:1761	NanoIGUR-loaded hydrogel	1738:1761	NanoIGUR-loaded hydrogel (10 mg/kg every 3 days)	1738:1785	Animal experiments confirmed that subcutaneous injection of NanoIGUR-loaded hydrogel (10 mg/kg every 3 days) and oral raw iguratimod (10mg/kg daily) showed similar efficacy in decreasing arthritis index score, pathological score, and expression of inflammatory cytokines.
31806967	9	94	theme	raw	1662:1664	arg1	iguratimod					1666:1675	oral raw iguratimod	1657:1675	oral raw iguratimod	1657:1675	The pharmacokinetic parameters showed better bioavailability and longer half-life time with NanoIGUR-loaded hydrogel by subcutaneous administration than oral raw iguratimod.
31806967	6	95	theme	LAP	1112:1114	arg1	technique					1116:1124	the LAP technique	1108:1124	the LAP technique	1108:1124	RESULTS By the LAP technique, we acquired the amorphous form nanoiguratimod, with an average size of 458 nm, which had higher dissolution rates and higher stability.
31806967	2	96	theme	NanoIGUR-loaded	376:390	arg1	hydrogel					366:373	nanoiguratimod-loaded hydrogel	344:373	nanoiguratimod-loaded hydrogel (NanoIGUR-loaded hydrogel) composites	344:411	To improve its bioavailability and to alleviate gastrointestinal side effects, we changed the formulation into nanoiguratimod-loaded hydrogel (NanoIGUR-loaded hydrogel) composites for sustained release of therapeutics.
31806967	2	96	theme	NanoIGUR-loaded	376:390	arg1	hydrogel					392:399	NanoIGUR-loaded hydrogel	376:399	NanoIGUR-loaded hydrogel	376:399	To improve its bioavailability and to alleviate gastrointestinal side effects, we changed the formulation into nanoiguratimod-loaded hydrogel (NanoIGUR-loaded hydrogel) composites for sustained release of therapeutics.
31869241	11	0	theme	acid	2156:2159	arg1	production					2161:2170	short-chain fatty acid production	2138:2170	short-chain fatty acid production	2138:2170	This study puts the importance of the (distal) colonic site in microbiota ecology forward, which should be considered in future therapeutic studies targeting, for instance, short-chain fatty acid production to improve metabolic health.
31869241	2	1	from	parameters	402:411	arg1	study					440:444	a human cross-sectional study	416:444	a human cross-sectional study	416:444	Here, we investigate to what extent quantitative measures of (segmental) colonic transit time were related to gut microbiota composition, microbial metabolites, and gut-related parameters in a human cross-sectional study.
31869241	6	2	theme	Long	1116:1119	arg1	transit					1155:1161	Long descending (i.e., distal) colonic transit	1116:1161	Long descending (i.e., distal) colonic transit	1116:1161	Long descending (i.e., distal) colonic transit was associated with increased microbial α-diversity but not with stool consistency.
31869241	3	3	theme	transit	493:499	arg1	CTT					507:509	CTT	507:509	CTT	507:509	Using radiopaque markers, (segmental) colonic transit time (CTT) was measured in 48 lean/overweight participants with long colonic transit but without constipation.
31869241	3	3	theme	transit	493:499	arg1	time					501:504	(segmental) colonic transit time	473:504	(segmental) colonic transit time (CTT)	473:510	Using radiopaque markers, (segmental) colonic transit time (CTT) was measured in 48 lean/overweight participants with long colonic transit but without constipation.
31869241	11	4	theme	short-chain	2138:2148	arg1	acid					2156:2159	short-chain fatty acid	2138:2159	short-chain fatty acid production	2138:2170	This study puts the importance of the (distal) colonic site in microbiota ecology forward, which should be considered in future therapeutic studies targeting, for instance, short-chain fatty acid production to improve metabolic health.
31869241	3	5	theme	colonic	485:491	arg1	CTT					507:509	CTT	507:509	CTT	507:509	Using radiopaque markers, (segmental) colonic transit time (CTT) was measured in 48 lean/overweight participants with long colonic transit but without constipation.
31869241	3	5	theme	colonic	485:491	arg1	time					501:504	(segmental) colonic transit time	473:504	(segmental) colonic transit time (CTT)	473:510	Using radiopaque markers, (segmental) colonic transit time (CTT) was measured in 48 lean/overweight participants with long colonic transit but without constipation.
31869241	11	6	theme	metabolic	2183:2191	arg1	health					2193:2198	metabolic health	2183:2198	metabolic health	2183:2198	This study puts the importance of the (distal) colonic site in microbiota ecology forward, which should be considered in future therapeutic studies targeting, for instance, short-chain fatty acid production to improve metabolic health.
31869241	6	7	theme	increased	1183:1191	arg1	α-diversity					1203:1213	increased microbial α-diversity	1183:1213	increased microbial α-diversity	1183:1213	Long descending (i.e., distal) colonic transit was associated with increased microbial α-diversity but not with stool consistency.
31869241	10	8	theme	microbiota	1666:1675	arg1	diversity					1677:1685	microbiota diversity	1666:1685	not only microbiota diversity but also microbial metabolism.NEW & NOTEWORTHY We extend previous findings showing that long distal colonic transit time influences microbial diversification and fermentation, whereas stool consistency is related to microbiota composition in humans with a long colonic transit	1657:1962	This study suggests that the distal colon transit may affect not only microbiota diversity but also microbial metabolism.NEW & NOTEWORTHY We extend previous findings showing that long distal colonic transit time influences microbial diversification and fermentation, whereas stool consistency is related to microbiota composition in humans with a long colonic transit.
31869241	10	9	theme	distal	1780:1785	arg1	time					1803:1806	long distal colonic transit time	1775:1806	long distal colonic transit time	1775:1806	This study suggests that the distal colon transit may affect not only microbiota diversity but also microbial metabolism.NEW & NOTEWORTHY We extend previous findings showing that long distal colonic transit time influences microbial diversification and fermentation, whereas stool consistency is related to microbiota composition in humans with a long colonic transit.
31869241	4	10	theme	rRNA	666:669	arg1	sequencing					685:694	16S rRNA gene amplicon sequencing	662:694	16S rRNA gene amplicon sequencing	662:694	Fecal microbiota composition was determined using 16S rRNA gene amplicon sequencing.
31869241	10	11	theme	distal	1625:1630	arg1	colon					1632:1636	the distal colon	1621:1636	the distal colon transit	1621:1644	This study suggests that the distal colon transit may affect not only microbiota diversity but also microbial metabolism.NEW & NOTEWORTHY We extend previous findings showing that long distal colonic transit time influences microbial diversification and fermentation, whereas stool consistency is related to microbiota composition in humans with a long colonic transit.
31869241	0	12	with	humans	91:96	arg1	transit					116:122	slow colonic transit	103:122	slow colonic transit	103:122	Distal colonic transit is linked to gut microbiota diversity and microbial fermentation in humans with slow colonic transit.
31869241	4	13	theme	microbiota	618:627	arg1	composition					629:639	Fecal microbiota composition	612:639	Fecal microbiota composition	612:639	Fecal microbiota composition was determined using 16S rRNA gene amplicon sequencing.
31869241	0	14	theme	colonic	108:114	arg1	transit					116:122	slow colonic transit	103:122	slow colonic transit	103:122	Distal colonic transit is linked to gut microbiota diversity and microbial fermentation in humans with slow colonic transit.
31869241	5	15	theme	univariate	1076:1085	arg1	approaches					1104:1113	univariate and multivariate approaches	1076:1113	univariate and multivariate approaches	1076:1113	Associations between gastrointestinal transit (segmental CTT and stool frequency and consistency), microbiota diversity and composition, microbial metabolites [short-chain fatty acids (SCFA), branched-chain fatty acids, and breath hydrogen], habitual diet, and gut-related host parameters [lipopolysaccharide-binding protein (LBP) and fecal calprotectin] were investigated using univariate and multivariate approaches.
31869241	5	16	theme	[short-chain	856:867	arg1	SCFA					882:885	SCFA	882:885	SCFA	882:885	Associations between gastrointestinal transit (segmental CTT and stool frequency and consistency), microbiota diversity and composition, microbial metabolites [short-chain fatty acids (SCFA), branched-chain fatty acids, and breath hydrogen], habitual diet, and gut-related host parameters [lipopolysaccharide-binding protein (LBP) and fecal calprotectin] were investigated using univariate and multivariate approaches.
31869241	5	16	theme	[short-chain	856:867	arg1	acids					875:879	[short-chain fatty acids	856:879	[short-chain fatty acids (SCFA)	856:886	Associations between gastrointestinal transit (segmental CTT and stool frequency and consistency), microbiota diversity and composition, microbial metabolites [short-chain fatty acids (SCFA), branched-chain fatty acids, and breath hydrogen], habitual diet, and gut-related host parameters [lipopolysaccharide-binding protein (LBP) and fecal calprotectin] were investigated using univariate and multivariate approaches.
31869241	11	17	theme	future	2086:2091	arg1	studies					2105:2111	future therapeutic studies	2086:2111	future therapeutic studies targeting, for instance, short-chain fatty acid production to improve metabolic health	2086:2198	This study puts the importance of the (distal) colonic site in microbiota ecology forward, which should be considered in future therapeutic studies targeting, for instance, short-chain fatty acid production to improve metabolic health.
31869241	5	18	theme	multivariate	1091:1102	arg1	approaches					1104:1113	univariate and multivariate approaches	1076:1113	univariate and multivariate approaches	1076:1113	Associations between gastrointestinal transit (segmental CTT and stool frequency and consistency), microbiota diversity and composition, microbial metabolites [short-chain fatty acids (SCFA), branched-chain fatty acids, and breath hydrogen], habitual diet, and gut-related host parameters [lipopolysaccharide-binding protein (LBP) and fecal calprotectin] were investigated using univariate and multivariate approaches.
31869241	1	19	theme	microbiota	203:212	arg1	diversity					214:222	increased gut microbiota diversity	189:222	increased gut microbiota diversity	189:222	Longer colonic transit time and hard stools are associated with increased gut microbiota diversity.
31869241	0	20	from	diversity	51:59	arg1	humans					91:96	humans	91:96	humans with slow colonic transit	91:122	Distal colonic transit is linked to gut microbiota diversity and microbial fermentation in humans with slow colonic transit.
31869241	3	21	theme	long	565:568	arg1	transit					578:584	long colonic transit	565:584	long colonic transit	565:584	Using radiopaque markers, (segmental) colonic transit time (CTT) was measured in 48 lean/overweight participants with long colonic transit but without constipation.
31869241	5	22	theme	breath	921:926	arg1	hydrogen					928:935	breath hydrogen	921:935	breath hydrogen	921:935	Associations between gastrointestinal transit (segmental CTT and stool frequency and consistency), microbiota diversity and composition, microbial metabolites [short-chain fatty acids (SCFA), branched-chain fatty acids, and breath hydrogen], habitual diet, and gut-related host parameters [lipopolysaccharide-binding protein (LBP) and fecal calprotectin] were investigated using univariate and multivariate approaches.
31869241	6	23	theme	stool	1228:1232	arg1	consistency					1234:1244	stool consistency	1228:1244	stool consistency	1228:1244	Long descending (i.e., distal) colonic transit was associated with increased microbial α-diversity but not with stool consistency.
31869241	11	24	theme	microbiota	2028:2037	arg1	ecology					2039:2045	microbiota ecology forward	2028:2053	microbiota ecology forward	2028:2053	This study puts the importance of the (distal) colonic site in microbiota ecology forward, which should be considered in future therapeutic studies targeting, for instance, short-chain fatty acid production to improve metabolic health.
31869241	1	25	theme	Longer	125:130	arg1	time					148:151	Longer colonic transit time	125:151	Longer colonic transit time	125:151	Longer colonic transit time and hard stools are associated with increased gut microbiota diversity.
31869241	1	26	theme	transit	140:146	arg1	time					148:151	Longer colonic transit time	125:151	Longer colonic transit time	125:151	Longer colonic transit time and hard stools are associated with increased gut microbiota diversity.
31869241	4	27	theme	amplicon	676:683	arg1	sequencing					685:694	16S rRNA gene amplicon sequencing	662:694	16S rRNA gene amplicon sequencing	662:694	Fecal microbiota composition was determined using 16S rRNA gene amplicon sequencing.
31869241	7	28	theme	unweighted	1253:1262	arg1	distance					1285:1292	unweighted and weighted UniFrac distance	1253:1292	distance	1285:1292	Using unweighted and weighted UniFrac distance, microbiota variation was not related to (segmental) CTT but to demographics, diet, plasma LBP, and fecal calprotectin.
31869241	5	29	theme	microbial	834:842	arg1	metabolites					844:854	microbial metabolites	834:854	microbial metabolites [short-chain fatty acids (SCFA), branched-chain fatty acids, and breath hydrogen]	834:936	Associations between gastrointestinal transit (segmental CTT and stool frequency and consistency), microbiota diversity and composition, microbial metabolites [short-chain fatty acids (SCFA), branched-chain fatty acids, and breath hydrogen], habitual diet, and gut-related host parameters [lipopolysaccharide-binding protein (LBP) and fecal calprotectin] were investigated using univariate and multivariate approaches.
31869241	9	30	theme	fecal	1551:1555	arg1	SCFA					1557:1560	fecal SCFA	1551:1560	fecal SCFA	1551:1560	Rectosigmoid and descending colonic transit were negatively associated with fecal SCFA and plasma acetate, respectively.
31869241	0	31	theme	microbial	65:73	arg1	fermentation					75:86	microbial fermentation	65:86	microbial fermentation	65:86	Distal colonic transit is linked to gut microbiota diversity and microbial fermentation in humans with slow colonic transit.
31869241	5	32	theme	segmental	744:752	arg1	CTT					754:756	segmental CTT	744:756	segmental CTT	744:756	Associations between gastrointestinal transit (segmental CTT and stool frequency and consistency), microbiota diversity and composition, microbial metabolites [short-chain fatty acids (SCFA), branched-chain fatty acids, and breath hydrogen], habitual diet, and gut-related host parameters [lipopolysaccharide-binding protein (LBP) and fecal calprotectin] were investigated using univariate and multivariate approaches.
31869241	10	33	theme	transit	1795:1801	arg1	time					1803:1806	long distal colonic transit time	1775:1806	long distal colonic transit time	1775:1806	This study suggests that the distal colon transit may affect not only microbiota diversity but also microbial metabolism.NEW & NOTEWORTHY We extend previous findings showing that long distal colonic transit time influences microbial diversification and fermentation, whereas stool consistency is related to microbiota composition in humans with a long colonic transit.
31869241	5	34	theme	gut-related	958:968	arg1	calprotectin					1038:1049	fecal calprotectin	1032:1049	fecal calprotectin	1032:1049	Associations between gastrointestinal transit (segmental CTT and stool frequency and consistency), microbiota diversity and composition, microbial metabolites [short-chain fatty acids (SCFA), branched-chain fatty acids, and breath hydrogen], habitual diet, and gut-related host parameters [lipopolysaccharide-binding protein (LBP) and fecal calprotectin] were investigated using univariate and multivariate approaches.
31869241	5	34	theme	gut-related	958:968	arg1	protein					1014:1020	[lipopolysaccharide-binding protein	986:1020	[lipopolysaccharide-binding protein (LBP)	986:1026	Associations between gastrointestinal transit (segmental CTT and stool frequency and consistency), microbiota diversity and composition, microbial metabolites [short-chain fatty acids (SCFA), branched-chain fatty acids, and breath hydrogen], habitual diet, and gut-related host parameters [lipopolysaccharide-binding protein (LBP) and fecal calprotectin] were investigated using univariate and multivariate approaches.
31869241	5	34	theme	gut-related	958:968	arg1	parameters					975:984	gut-related host parameters [lipopolysaccharide-binding protein (LBP) and fecal calprotectin	958:1049	gut-related host parameters [lipopolysaccharide-binding protein (LBP) and fecal calprotectin	958:1049	Associations between gastrointestinal transit (segmental CTT and stool frequency and consistency), microbiota diversity and composition, microbial metabolites [short-chain fatty acids (SCFA), branched-chain fatty acids, and breath hydrogen], habitual diet, and gut-related host parameters [lipopolysaccharide-binding protein (LBP) and fecal calprotectin] were investigated using univariate and multivariate approaches.
31869241	2	35	theme	microbial	363:371	arg1	metabolites					373:383	microbial metabolites	363:383	microbial metabolites	363:383	Here, we investigate to what extent quantitative measures of (segmental) colonic transit time were related to gut microbiota composition, microbial metabolites, and gut-related parameters in a human cross-sectional study.
31869241	0	36	theme	Distal	0:5	arg1	transit					15:21	Distal colonic transit	0:21	Distal colonic transit	0:21	Distal colonic transit is linked to gut microbiota diversity and microbial fermentation in humans with slow colonic transit.
31869241	5	37	theme	habitual	939:946	arg1	diet					948:951	habitual diet	939:951	habitual diet	939:951	Associations between gastrointestinal transit (segmental CTT and stool frequency and consistency), microbiota diversity and composition, microbial metabolites [short-chain fatty acids (SCFA), branched-chain fatty acids, and breath hydrogen], habitual diet, and gut-related host parameters [lipopolysaccharide-binding protein (LBP) and fecal calprotectin] were investigated using univariate and multivariate approaches.
31869241	2	38	theme	human	418:422	arg1	study					440:444	a human cross-sectional study	416:444	a human cross-sectional study	416:444	Here, we investigate to what extent quantitative measures of (segmental) colonic transit time were related to gut microbiota composition, microbial metabolites, and gut-related parameters in a human cross-sectional study.
31869241	3	39	theme	radiopaque	453:462	arg1	markers					464:470	radiopaque markers	453:470	radiopaque markers	453:470	Using radiopaque markers, (segmental) colonic transit time (CTT) was measured in 48 lean/overweight participants with long colonic transit but without constipation.
31869241	3	40	with	participants	547:558	arg1	transit					578:584	long colonic transit	565:584	long colonic transit	565:584	Using radiopaque markers, (segmental) colonic transit time (CTT) was measured in 48 lean/overweight participants with long colonic transit but without constipation.
31869241	10	41	theme	colonic	1787:1793	arg1	time					1803:1806	long distal colonic transit time	1775:1806	long distal colonic transit time	1775:1806	This study suggests that the distal colon transit may affect not only microbiota diversity but also microbial metabolism.NEW & NOTEWORTHY We extend previous findings showing that long distal colonic transit time influences microbial diversification and fermentation, whereas stool consistency is related to microbiota composition in humans with a long colonic transit.
31869241	1	42	theme	colonic	132:138	arg1	time					148:151	Longer colonic transit time	125:151	Longer colonic transit time	125:151	Longer colonic transit time and hard stools are associated with increased gut microbiota diversity.
31869241	11	43	from	site	2020:2023	arg1	ecology					2039:2045	microbiota ecology forward	2028:2053	microbiota ecology forward	2028:2053	This study puts the importance of the (distal) colonic site in microbiota ecology forward, which should be considered in future therapeutic studies targeting, for instance, short-chain fatty acid production to improve metabolic health.
31869241	10	44	theme	microbial	1696:1704	arg1	NOTEWORTHY					1723:1732	microbial metabolism.NEW & NOTEWORTHY	1696:1732	not only microbiota diversity but also microbial metabolism.NEW & NOTEWORTHY We extend previous findings showing that long distal colonic transit time influences microbial diversification and fermentation, whereas stool consistency is related to microbiota composition in humans with a long colonic transit	1657:1962	This study suggests that the distal colon transit may affect not only microbiota diversity but also microbial metabolism.NEW & NOTEWORTHY We extend previous findings showing that long distal colonic transit time influences microbial diversification and fermentation, whereas stool consistency is related to microbiota composition in humans with a long colonic transit.
31869241	2	45	theme	gut-related	390:400	arg1	parameters					402:411	gut-related parameters	390:411	gut-related parameters	390:411	Here, we investigate to what extent quantitative measures of (segmental) colonic transit time were related to gut microbiota composition, microbial metabolites, and gut-related parameters in a human cross-sectional study.
31869241	10	46	theme	microbiota	1903:1912	arg1	composition					1914:1924	microbiota composition	1903:1924	microbiota composition in humans with a long colonic transit	1903:1962	This study suggests that the distal colon transit may affect not only microbiota diversity but also microbial metabolism.NEW & NOTEWORTHY We extend previous findings showing that long distal colonic transit time influences microbial diversification and fermentation, whereas stool consistency is related to microbiota composition in humans with a long colonic transit.
31869241	3	47	theme	segmental	474:482	arg1	CTT					507:509	CTT	507:509	CTT	507:509	Using radiopaque markers, (segmental) colonic transit time (CTT) was measured in 48 lean/overweight participants with long colonic transit but without constipation.
31869241	3	47	theme	segmental	474:482	arg1	time					501:504	(segmental) colonic transit time	473:504	(segmental) colonic transit time (CTT)	473:510	Using radiopaque markers, (segmental) colonic transit time (CTT) was measured in 48 lean/overweight participants with long colonic transit but without constipation.
31869241	0	48	theme	gut	36:38	arg1	diversity					51:59	gut microbiota diversity	36:59	gut microbiota diversity	36:59	Distal colonic transit is linked to gut microbiota diversity and microbial fermentation in humans with slow colonic transit.
31869241	11	49	theme	site	2020:2023	arg1	importance					1985:1994	the importance	1981:1994	the importance of the (distal) colonic site in microbiota ecology forward, which should be considered in future therapeutic studies targeting, for instance, short-chain fatty acid production to improve metabolic health	1981:2198	This study puts the importance of the (distal) colonic site in microbiota ecology forward, which should be considered in future therapeutic studies targeting, for instance, short-chain fatty acid production to improve metabolic health.
31869241	10	50	theme	long	1943:1946	arg1	transit					1956:1962	a long colonic transit	1941:1962	a long colonic transit	1941:1962	This study suggests that the distal colon transit may affect not only microbiota diversity but also microbial metabolism.NEW & NOTEWORTHY We extend previous findings showing that long distal colonic transit time influences microbial diversification and fermentation, whereas stool consistency is related to microbiota composition in humans with a long colonic transit.
31869241	2	51	theme	time	314:317	arg1	measures					274:281	quantitative measures	261:281	quantitative measures of (segmental) colonic transit time	261:317	Here, we investigate to what extent quantitative measures of (segmental) colonic transit time were related to gut microbiota composition, microbial metabolites, and gut-related parameters in a human cross-sectional study.
31869241	11	52	theme	distal	2004:2009	arg1	site					2020:2023	the (distal) colonic site	1999:2023	the (distal) colonic site	1999:2023	This study puts the importance of the (distal) colonic site in microbiota ecology forward, which should be considered in future therapeutic studies targeting, for instance, short-chain fatty acid production to improve metabolic health.
31869241	5	53	theme	fatty	904:908	arg1	acids					910:914	branched-chain fatty acids	889:914	branched-chain fatty acids	889:914	Associations between gastrointestinal transit (segmental CTT and stool frequency and consistency), microbiota diversity and composition, microbial metabolites [short-chain fatty acids (SCFA), branched-chain fatty acids, and breath hydrogen], habitual diet, and gut-related host parameters [lipopolysaccharide-binding protein (LBP) and fecal calprotectin] were investigated using univariate and multivariate approaches.
31869241	11	54	dep	future	2086:2091	arg1	therapeutic					2093:2103	therapeutic	2093:2103	therapeutic	2093:2103	This study puts the importance of the (distal) colonic site in microbiota ecology forward, which should be considered in future therapeutic studies targeting, for instance, short-chain fatty acid production to improve metabolic health.
31869241	5	55	dep	metabolites	844:854	arg1	acids					910:914	branched-chain fatty acids	889:914	branched-chain fatty acids	889:914	Associations between gastrointestinal transit (segmental CTT and stool frequency and consistency), microbiota diversity and composition, microbial metabolites [short-chain fatty acids (SCFA), branched-chain fatty acids, and breath hydrogen], habitual diet, and gut-related host parameters [lipopolysaccharide-binding protein (LBP) and fecal calprotectin] were investigated using univariate and multivariate approaches.
31869241	5	55	dep	metabolites	844:854	arg1	SCFA					882:885	SCFA	882:885	SCFA	882:885	Associations between gastrointestinal transit (segmental CTT and stool frequency and consistency), microbiota diversity and composition, microbial metabolites [short-chain fatty acids (SCFA), branched-chain fatty acids, and breath hydrogen], habitual diet, and gut-related host parameters [lipopolysaccharide-binding protein (LBP) and fecal calprotectin] were investigated using univariate and multivariate approaches.
31869241	5	55	dep	metabolites	844:854	arg1	acids					875:879	[short-chain fatty acids	856:879	[short-chain fatty acids (SCFA)	856:886	Associations between gastrointestinal transit (segmental CTT and stool frequency and consistency), microbiota diversity and composition, microbial metabolites [short-chain fatty acids (SCFA), branched-chain fatty acids, and breath hydrogen], habitual diet, and gut-related host parameters [lipopolysaccharide-binding protein (LBP) and fecal calprotectin] were investigated using univariate and multivariate approaches.
31869241	5	55	dep	metabolites	844:854	arg1	hydrogen					928:935	breath hydrogen	921:935	breath hydrogen	921:935	Associations between gastrointestinal transit (segmental CTT and stool frequency and consistency), microbiota diversity and composition, microbial metabolites [short-chain fatty acids (SCFA), branched-chain fatty acids, and breath hydrogen], habitual diet, and gut-related host parameters [lipopolysaccharide-binding protein (LBP) and fecal calprotectin] were investigated using univariate and multivariate approaches.
31869241	10	56	theme	long	1775:1778	arg1	time					1803:1806	long distal colonic transit time	1775:1806	long distal colonic transit time	1775:1806	This study suggests that the distal colon transit may affect not only microbiota diversity but also microbial metabolism.NEW & NOTEWORTHY We extend previous findings showing that long distal colonic transit time influences microbial diversification and fermentation, whereas stool consistency is related to microbiota composition in humans with a long colonic transit.
31869241	5	57	theme	microbiota	796:805	arg1	diversity					807:815	microbiota diversity	796:815	microbiota diversity	796:815	Associations between gastrointestinal transit (segmental CTT and stool frequency and consistency), microbiota diversity and composition, microbial metabolites [short-chain fatty acids (SCFA), branched-chain fatty acids, and breath hydrogen], habitual diet, and gut-related host parameters [lipopolysaccharide-binding protein (LBP) and fecal calprotectin] were investigated using univariate and multivariate approaches.
31869241	6	58	theme	descending	1121:1130	arg1	transit					1155:1161	Long descending (i.e., distal) colonic transit	1116:1161	Long descending (i.e., distal) colonic transit	1116:1161	Long descending (i.e., distal) colonic transit was associated with increased microbial α-diversity but not with stool consistency.
31869241	11	59	from	ecology	2039:2045	arg1	importance					1985:1994	the importance	1981:1994	the importance of the (distal) colonic site in microbiota ecology forward, which should be considered in future therapeutic studies targeting, for instance, short-chain fatty acid production to improve metabolic health	1981:2198	This study puts the importance of the (distal) colonic site in microbiota ecology forward, which should be considered in future therapeutic studies targeting, for instance, short-chain fatty acid production to improve metabolic health.
31869241	2	60	theme	gut	335:337	arg1	composition					350:360	gut microbiota composition	335:360	gut microbiota composition	335:360	Here, we investigate to what extent quantitative measures of (segmental) colonic transit time were related to gut microbiota composition, microbial metabolites, and gut-related parameters in a human cross-sectional study.
31869241	7	61	theme	weighted	1268:1275	arg1	distance					1285:1292	unweighted and weighted UniFrac distance	1253:1292	distance	1285:1292	Using unweighted and weighted UniFrac distance, microbiota variation was not related to (segmental) CTT but to demographics, diet, plasma LBP, and fecal calprotectin.
31869241	2	62	theme	colonic	298:304	arg1	time					314:317	(segmental) colonic transit time	286:317	(segmental) colonic transit time	286:317	Here, we investigate to what extent quantitative measures of (segmental) colonic transit time were related to gut microbiota composition, microbial metabolites, and gut-related parameters in a human cross-sectional study.
31869241	11	63	theme	fatty	2150:2154	arg1	acid					2156:2159	short-chain fatty acid	2138:2159	short-chain fatty acid production	2138:2170	This study puts the importance of the (distal) colonic site in microbiota ecology forward, which should be considered in future therapeutic studies targeting, for instance, short-chain fatty acid production to improve metabolic health.
31869241	6	64	theme	microbial	1193:1201	arg1	α-diversity					1203:1213	increased microbial α-diversity	1183:1213	increased microbial α-diversity	1183:1213	Long descending (i.e., distal) colonic transit was associated with increased microbial α-diversity but not with stool consistency.
31869241	10	65	theme	previous	1744:1751	arg1	findings					1753:1760	previous findings	1744:1760	previous findings showing that long distal colonic transit time influences microbial diversification and fermentation, whereas stool consistency is related to microbiota composition in humans with a long colonic transit	1744:1962	This study suggests that the distal colon transit may affect not only microbiota diversity but also microbial metabolism.NEW & NOTEWORTHY We extend previous findings showing that long distal colonic transit time influences microbial diversification and fermentation, whereas stool consistency is related to microbiota composition in humans with a long colonic transit.
31869241	2	66	theme	quantitative	261:272	arg1	measures					274:281	quantitative measures	261:281	quantitative measures of (segmental) colonic transit time	261:317	Here, we investigate to what extent quantitative measures of (segmental) colonic transit time were related to gut microbiota composition, microbial metabolites, and gut-related parameters in a human cross-sectional study.
31869241	1	67	theme	hard	157:160	arg1	stools					162:167	hard stools	157:167	hard stools	157:167	Longer colonic transit time and hard stools are associated with increased gut microbiota diversity.
31869241	0	68	attach	linked	26:31	arg1	diversity					51:59	gut microbiota diversity	36:59	gut microbiota diversity	36:59	Distal colonic transit is linked to gut microbiota diversity and microbial fermentation in humans with slow colonic transit.
31869241	0	68	attach	linked	26:31	arg1	fermentation					75:86	microbial fermentation	65:86	microbial fermentation	65:86	Distal colonic transit is linked to gut microbiota diversity and microbial fermentation in humans with slow colonic transit.
31869241	0	68	attach	linked	26:31	arg2	transit					15:21	Distal colonic transit	0:21	Distal colonic transit	0:21	Distal colonic transit is linked to gut microbiota diversity and microbial fermentation in humans with slow colonic transit.
31869241	10	69	theme	colon	1632:1636	arg1	transit					1638:1644	the distal colon transit	1621:1644	the distal colon transit	1621:1644	This study suggests that the distal colon transit may affect not only microbiota diversity but also microbial metabolism.NEW & NOTEWORTHY We extend previous findings showing that long distal colonic transit time influences microbial diversification and fermentation, whereas stool consistency is related to microbiota composition in humans with a long colonic transit.
31869241	3	70	theme	lean/overweight	531:545	arg1	participants					547:558	48 lean/overweight participants	528:558	48 lean/overweight participants with long colonic transit but without constipation	528:609	Using radiopaque markers, (segmental) colonic transit time (CTT) was measured in 48 lean/overweight participants with long colonic transit but without constipation.
31869241	2	71	from	composition	350:360	arg1	study					440:444	a human cross-sectional study	416:444	a human cross-sectional study	416:444	Here, we investigate to what extent quantitative measures of (segmental) colonic transit time were related to gut microbiota composition, microbial metabolites, and gut-related parameters in a human cross-sectional study.
31869241	4	72	theme	16S	662:664	arg1	sequencing					685:694	16S rRNA gene amplicon sequencing	662:694	16S rRNA gene amplicon sequencing	662:694	Fecal microbiota composition was determined using 16S rRNA gene amplicon sequencing.
31869241	10	73	theme	&	1721:1721	arg1	NOTEWORTHY					1723:1732	microbial metabolism.NEW & NOTEWORTHY	1696:1732	not only microbiota diversity but also microbial metabolism.NEW & NOTEWORTHY We extend previous findings showing that long distal colonic transit time influences microbial diversification and fermentation, whereas stool consistency is related to microbiota composition in humans with a long colonic transit	1657:1962	This study suggests that the distal colon transit may affect not only microbiota diversity but also microbial metabolism.NEW & NOTEWORTHY We extend previous findings showing that long distal colonic transit time influences microbial diversification and fermentation, whereas stool consistency is related to microbiota composition in humans with a long colonic transit.
31869241	2	74	theme	segmental	287:295	arg1	time					314:317	(segmental) colonic transit time	286:317	(segmental) colonic transit time	286:317	Here, we investigate to what extent quantitative measures of (segmental) colonic transit time were related to gut microbiota composition, microbial metabolites, and gut-related parameters in a human cross-sectional study.
31869241	9	75	theme	Rectosigmoid	1475:1486	arg1	transit					1511:1517	Rectosigmoid and descending colonic transit	1475:1517	Rectosigmoid and descending colonic transit	1475:1517	Rectosigmoid and descending colonic transit were negatively associated with fecal SCFA and plasma acetate, respectively.
31869241	0	76	from	fermentation	75:86	arg1	humans					91:96	humans	91:96	humans with slow colonic transit	91:122	Distal colonic transit is linked to gut microbiota diversity and microbial fermentation in humans with slow colonic transit.
31869241	6	77	theme	colonic	1147:1153	arg1	transit					1155:1161	Long descending (i.e., distal) colonic transit	1116:1161	Long descending (i.e., distal) colonic transit	1116:1161	Long descending (i.e., distal) colonic transit was associated with increased microbial α-diversity but not with stool consistency.
31869241	4	78	theme	Fecal	612:616	arg1	composition					629:639	Fecal microbiota composition	612:639	Fecal microbiota composition	612:639	Fecal microbiota composition was determined using 16S rRNA gene amplicon sequencing.
31869241	5	79	theme	gastrointestinal	718:733	arg1	transit					735:741	gastrointestinal transit	718:741	gastrointestinal transit (segmental CTT and stool frequency and consistency)	718:793	Associations between gastrointestinal transit (segmental CTT and stool frequency and consistency), microbiota diversity and composition, microbial metabolites [short-chain fatty acids (SCFA), branched-chain fatty acids, and breath hydrogen], habitual diet, and gut-related host parameters [lipopolysaccharide-binding protein (LBP) and fecal calprotectin] were investigated using univariate and multivariate approaches.
31869241	3	80	theme	colonic	570:576	arg1	transit					578:584	long colonic transit	565:584	long colonic transit	565:584	Using radiopaque markers, (segmental) colonic transit time (CTT) was measured in 48 lean/overweight participants with long colonic transit but without constipation.
31869241	5	81	theme	fatty	869:873	arg1	SCFA					882:885	SCFA	882:885	SCFA	882:885	Associations between gastrointestinal transit (segmental CTT and stool frequency and consistency), microbiota diversity and composition, microbial metabolites [short-chain fatty acids (SCFA), branched-chain fatty acids, and breath hydrogen], habitual diet, and gut-related host parameters [lipopolysaccharide-binding protein (LBP) and fecal calprotectin] were investigated using univariate and multivariate approaches.
31869241	5	81	theme	fatty	869:873	arg1	acids					875:879	[short-chain fatty acids	856:879	[short-chain fatty acids (SCFA)	856:886	Associations between gastrointestinal transit (segmental CTT and stool frequency and consistency), microbiota diversity and composition, microbial metabolites [short-chain fatty acids (SCFA), branched-chain fatty acids, and breath hydrogen], habitual diet, and gut-related host parameters [lipopolysaccharide-binding protein (LBP) and fecal calprotectin] were investigated using univariate and multivariate approaches.
31869241	7	82	theme	plasma	1378:1383	arg1	LBP					1385:1387	plasma LBP	1378:1387	plasma LBP	1378:1387	Using unweighted and weighted UniFrac distance, microbiota variation was not related to (segmental) CTT but to demographics, diet, plasma LBP, and fecal calprotectin.
31869241	11	83	from	importance	1985:1994	arg1	ecology					2039:2045	microbiota ecology forward	2028:2053	microbiota ecology forward	2028:2053	This study puts the importance of the (distal) colonic site in microbiota ecology forward, which should be considered in future therapeutic studies targeting, for instance, short-chain fatty acid production to improve metabolic health.
31869241	5	84	dep	parameters	975:984	arg1	LBP					1023:1025	LBP	1023:1025	LBP	1023:1025	Associations between gastrointestinal transit (segmental CTT and stool frequency and consistency), microbiota diversity and composition, microbial metabolites [short-chain fatty acids (SCFA), branched-chain fatty acids, and breath hydrogen], habitual diet, and gut-related host parameters [lipopolysaccharide-binding protein (LBP) and fecal calprotectin] were investigated using univariate and multivariate approaches.
31869241	5	84	dep	parameters	975:984	arg1	parameters					975:984	gut-related host parameters [lipopolysaccharide-binding protein (LBP) and fecal calprotectin	958:1049	gut-related host parameters [lipopolysaccharide-binding protein (LBP) and fecal calprotectin	958:1049	Associations between gastrointestinal transit (segmental CTT and stool frequency and consistency), microbiota diversity and composition, microbial metabolites [short-chain fatty acids (SCFA), branched-chain fatty acids, and breath hydrogen], habitual diet, and gut-related host parameters [lipopolysaccharide-binding protein (LBP) and fecal calprotectin] were investigated using univariate and multivariate approaches.
31869241	5	84	dep	parameters	975:984	arg1	protein					1014:1020	[lipopolysaccharide-binding protein	986:1020	[lipopolysaccharide-binding protein (LBP)	986:1026	Associations between gastrointestinal transit (segmental CTT and stool frequency and consistency), microbiota diversity and composition, microbial metabolites [short-chain fatty acids (SCFA), branched-chain fatty acids, and breath hydrogen], habitual diet, and gut-related host parameters [lipopolysaccharide-binding protein (LBP) and fecal calprotectin] were investigated using univariate and multivariate approaches.
31869241	5	84	dep	parameters	975:984	arg1	calprotectin					1038:1049	fecal calprotectin	1032:1049	fecal calprotectin	1032:1049	Associations between gastrointestinal transit (segmental CTT and stool frequency and consistency), microbiota diversity and composition, microbial metabolites [short-chain fatty acids (SCFA), branched-chain fatty acids, and breath hydrogen], habitual diet, and gut-related host parameters [lipopolysaccharide-binding protein (LBP) and fecal calprotectin] were investigated using univariate and multivariate approaches.
31869241	10	85	theme	microbial	1819:1827	arg1	diversification					1829:1843	microbial diversification and fermentation	1819:1860	diversification	1829:1843	This study suggests that the distal colon transit may affect not only microbiota diversity but also microbial metabolism.NEW & NOTEWORTHY We extend previous findings showing that long distal colonic transit time influences microbial diversification and fermentation, whereas stool consistency is related to microbiota composition in humans with a long colonic transit.
31869241	5	86	theme	stool	762:766	arg1	frequency					768:776	stool frequency	762:776	stool frequency	762:776	Associations between gastrointestinal transit (segmental CTT and stool frequency and consistency), microbiota diversity and composition, microbial metabolites [short-chain fatty acids (SCFA), branched-chain fatty acids, and breath hydrogen], habitual diet, and gut-related host parameters [lipopolysaccharide-binding protein (LBP) and fecal calprotectin] were investigated using univariate and multivariate approaches.
31869241	11	87	theme	colonic	2012:2018	arg1	site					2020:2023	the (distal) colonic site	1999:2023	the (distal) colonic site	1999:2023	This study puts the importance of the (distal) colonic site in microbiota ecology forward, which should be considered in future therapeutic studies targeting, for instance, short-chain fatty acid production to improve metabolic health.
31869241	5	88	theme	branched-chain	889:902	arg1	acids					910:914	branched-chain fatty acids	889:914	branched-chain fatty acids	889:914	Associations between gastrointestinal transit (segmental CTT and stool frequency and consistency), microbiota diversity and composition, microbial metabolites [short-chain fatty acids (SCFA), branched-chain fatty acids, and breath hydrogen], habitual diet, and gut-related host parameters [lipopolysaccharide-binding protein (LBP) and fecal calprotectin] were investigated using univariate and multivariate approaches.
31869241	5	89	dep	transit	735:741	arg1	CTT					754:756	segmental CTT	744:756	segmental CTT	744:756	Associations between gastrointestinal transit (segmental CTT and stool frequency and consistency), microbiota diversity and composition, microbial metabolites [short-chain fatty acids (SCFA), branched-chain fatty acids, and breath hydrogen], habitual diet, and gut-related host parameters [lipopolysaccharide-binding protein (LBP) and fecal calprotectin] were investigated using univariate and multivariate approaches.
31869241	5	89	dep	transit	735:741	arg1	frequency					768:776	stool frequency	762:776	stool frequency	762:776	Associations between gastrointestinal transit (segmental CTT and stool frequency and consistency), microbiota diversity and composition, microbial metabolites [short-chain fatty acids (SCFA), branched-chain fatty acids, and breath hydrogen], habitual diet, and gut-related host parameters [lipopolysaccharide-binding protein (LBP) and fecal calprotectin] were investigated using univariate and multivariate approaches.
31869241	5	89	dep	transit	735:741	arg1	consistency					782:792	consistency	782:792	consistency	782:792	Associations between gastrointestinal transit (segmental CTT and stool frequency and consistency), microbiota diversity and composition, microbial metabolites [short-chain fatty acids (SCFA), branched-chain fatty acids, and breath hydrogen], habitual diet, and gut-related host parameters [lipopolysaccharide-binding protein (LBP) and fecal calprotectin] were investigated using univariate and multivariate approaches.
31869241	4	90	theme	gene	671:674	arg1	sequencing					685:694	16S rRNA gene amplicon sequencing	662:694	16S rRNA gene amplicon sequencing	662:694	Fecal microbiota composition was determined using 16S rRNA gene amplicon sequencing.
31869241	8	91	theme	stool	1456:1460	arg1	consistency					1462:1472	stool consistency	1456:1472	stool consistency	1456:1472	Bray-Curtis dissimilarity related only to stool consistency.
31869241	9	92	theme	plasma	1566:1571	arg1	acetate					1573:1579	plasma acetate	1566:1579	plasma acetate	1566:1579	Rectosigmoid and descending colonic transit were negatively associated with fecal SCFA and plasma acetate, respectively.
31869241	7	93	theme	fecal	1394:1398	arg1	calprotectin					1400:1411	fecal calprotectin	1394:1411	fecal calprotectin	1394:1411	Using unweighted and weighted UniFrac distance, microbiota variation was not related to (segmental) CTT but to demographics, diet, plasma LBP, and fecal calprotectin.
31869241	7	94	theme	microbiota	1295:1304	arg1	variation					1306:1314	microbiota variation	1295:1314	microbiota variation	1295:1314	Using unweighted and weighted UniFrac distance, microbiota variation was not related to (segmental) CTT but to demographics, diet, plasma LBP, and fecal calprotectin.
31869241	0	95	theme	slow	103:106	arg1	transit					116:122	slow colonic transit	103:122	slow colonic transit	103:122	Distal colonic transit is linked to gut microbiota diversity and microbial fermentation in humans with slow colonic transit.
31869241	1	96	theme	increased	189:197	arg1	diversity					214:222	increased gut microbiota diversity	189:222	increased gut microbiota diversity	189:222	Longer colonic transit time and hard stools are associated with increased gut microbiota diversity.
31869241	5	97	theme	host	970:973	arg1	calprotectin					1038:1049	fecal calprotectin	1032:1049	fecal calprotectin	1032:1049	Associations between gastrointestinal transit (segmental CTT and stool frequency and consistency), microbiota diversity and composition, microbial metabolites [short-chain fatty acids (SCFA), branched-chain fatty acids, and breath hydrogen], habitual diet, and gut-related host parameters [lipopolysaccharide-binding protein (LBP) and fecal calprotectin] were investigated using univariate and multivariate approaches.
31869241	5	97	theme	host	970:973	arg1	protein					1014:1020	[lipopolysaccharide-binding protein	986:1020	[lipopolysaccharide-binding protein (LBP)	986:1026	Associations between gastrointestinal transit (segmental CTT and stool frequency and consistency), microbiota diversity and composition, microbial metabolites [short-chain fatty acids (SCFA), branched-chain fatty acids, and breath hydrogen], habitual diet, and gut-related host parameters [lipopolysaccharide-binding protein (LBP) and fecal calprotectin] were investigated using univariate and multivariate approaches.
31869241	5	97	theme	host	970:973	arg1	parameters					975:984	gut-related host parameters [lipopolysaccharide-binding protein (LBP) and fecal calprotectin	958:1049	gut-related host parameters [lipopolysaccharide-binding protein (LBP) and fecal calprotectin	958:1049	Associations between gastrointestinal transit (segmental CTT and stool frequency and consistency), microbiota diversity and composition, microbial metabolites [short-chain fatty acids (SCFA), branched-chain fatty acids, and breath hydrogen], habitual diet, and gut-related host parameters [lipopolysaccharide-binding protein (LBP) and fecal calprotectin] were investigated using univariate and multivariate approaches.
31869241	1	98	theme	gut	199:201	arg1	diversity					214:222	increased gut microbiota diversity	189:222	increased gut microbiota diversity	189:222	Longer colonic transit time and hard stools are associated with increased gut microbiota diversity.
31869241	0	99	theme	colonic	7:13	arg1	transit					15:21	Distal colonic transit	0:21	Distal colonic transit	0:21	Distal colonic transit is linked to gut microbiota diversity and microbial fermentation in humans with slow colonic transit.
31869241	7	100	theme	segmental	1336:1344	arg1	CTT					1347:1349	(segmental) CTT	1335:1349	(segmental) CTT	1335:1349	Using unweighted and weighted UniFrac distance, microbiota variation was not related to (segmental) CTT but to demographics, diet, plasma LBP, and fecal calprotectin.
31869241	2	101	theme	cross-sectional	424:438	arg1	study					440:444	a human cross-sectional study	416:444	a human cross-sectional study	416:444	Here, we investigate to what extent quantitative measures of (segmental) colonic transit time were related to gut microbiota composition, microbial metabolites, and gut-related parameters in a human cross-sectional study.
31869241	10	102	dep	NOTEWORTHY	1723:1732	arg1	extend					1737:1742	extend	1737:1742	extend previous findings showing that long distal colonic transit time influences microbial diversification and fermentation, whereas stool consistency is related to microbiota composition in humans with a long colonic transit	1737:1962	This study suggests that the distal colon transit may affect not only microbiota diversity but also microbial metabolism.NEW & NOTEWORTHY We extend previous findings showing that long distal colonic transit time influences microbial diversification and fermentation, whereas stool consistency is related to microbiota composition in humans with a long colonic transit.
31869241	10	103	theme	stool	1871:1875	arg1	consistency					1877:1887	stool consistency	1871:1887	stool consistency	1871:1887	This study suggests that the distal colon transit may affect not only microbiota diversity but also microbial metabolism.NEW & NOTEWORTHY We extend previous findings showing that long distal colonic transit time influences microbial diversification and fermentation, whereas stool consistency is related to microbiota composition in humans with a long colonic transit.
31869241	10	104	theme	metabolism.NEW	1706:1719	arg1	NOTEWORTHY					1723:1732	microbial metabolism.NEW & NOTEWORTHY	1696:1732	not only microbiota diversity but also microbial metabolism.NEW & NOTEWORTHY We extend previous findings showing that long distal colonic transit time influences microbial diversification and fermentation, whereas stool consistency is related to microbiota composition in humans with a long colonic transit	1657:1962	This study suggests that the distal colon transit may affect not only microbiota diversity but also microbial metabolism.NEW & NOTEWORTHY We extend previous findings showing that long distal colonic transit time influences microbial diversification and fermentation, whereas stool consistency is related to microbiota composition in humans with a long colonic transit.
31869241	9	105	theme	descending	1492:1501	arg1	transit					1511:1517	Rectosigmoid and descending colonic transit	1475:1517	Rectosigmoid and descending colonic transit	1475:1517	Rectosigmoid and descending colonic transit were negatively associated with fecal SCFA and plasma acetate, respectively.
31869241	0	106	theme	microbiota	40:49	arg1	diversity					51:59	gut microbiota diversity	36:59	gut microbiota diversity	36:59	Distal colonic transit is linked to gut microbiota diversity and microbial fermentation in humans with slow colonic transit.
31869241	5	107	theme	fecal	1032:1036	arg1	parameters					975:984	gut-related host parameters [lipopolysaccharide-binding protein (LBP) and fecal calprotectin	958:1049	gut-related host parameters [lipopolysaccharide-binding protein (LBP) and fecal calprotectin	958:1049	Associations between gastrointestinal transit (segmental CTT and stool frequency and consistency), microbiota diversity and composition, microbial metabolites [short-chain fatty acids (SCFA), branched-chain fatty acids, and breath hydrogen], habitual diet, and gut-related host parameters [lipopolysaccharide-binding protein (LBP) and fecal calprotectin] were investigated using univariate and multivariate approaches.
31869241	5	107	theme	fecal	1032:1036	arg1	calprotectin					1038:1049	fecal calprotectin	1032:1049	fecal calprotectin	1032:1049	Associations between gastrointestinal transit (segmental CTT and stool frequency and consistency), microbiota diversity and composition, microbial metabolites [short-chain fatty acids (SCFA), branched-chain fatty acids, and breath hydrogen], habitual diet, and gut-related host parameters [lipopolysaccharide-binding protein (LBP) and fecal calprotectin] were investigated using univariate and multivariate approaches.
31869241	10	108	theme	colonic	1948:1954	arg1	transit					1956:1962	a long colonic transit	1941:1962	a long colonic transit	1941:1962	This study suggests that the distal colon transit may affect not only microbiota diversity but also microbial metabolism.NEW & NOTEWORTHY We extend previous findings showing that long distal colonic transit time influences microbial diversification and fermentation, whereas stool consistency is related to microbiota composition in humans with a long colonic transit.
31869241	6	109	dep	descending	1121:1130	arg1	distal					1139:1144	distal	1139:1144	distal	1139:1144	Long descending (i.e., distal) colonic transit was associated with increased microbial α-diversity but not with stool consistency.
31869241	5	110	theme	[lipopolysaccharide-binding	986:1012	arg1	LBP					1023:1025	LBP	1023:1025	LBP	1023:1025	Associations between gastrointestinal transit (segmental CTT and stool frequency and consistency), microbiota diversity and composition, microbial metabolites [short-chain fatty acids (SCFA), branched-chain fatty acids, and breath hydrogen], habitual diet, and gut-related host parameters [lipopolysaccharide-binding protein (LBP) and fecal calprotectin] were investigated using univariate and multivariate approaches.
31869241	5	110	theme	[lipopolysaccharide-binding	986:1012	arg1	protein					1014:1020	[lipopolysaccharide-binding protein	986:1020	[lipopolysaccharide-binding protein (LBP)	986:1026	Associations between gastrointestinal transit (segmental CTT and stool frequency and consistency), microbiota diversity and composition, microbial metabolites [short-chain fatty acids (SCFA), branched-chain fatty acids, and breath hydrogen], habitual diet, and gut-related host parameters [lipopolysaccharide-binding protein (LBP) and fecal calprotectin] were investigated using univariate and multivariate approaches.
31869241	5	110	theme	[lipopolysaccharide-binding	986:1012	arg1	parameters					975:984	gut-related host parameters [lipopolysaccharide-binding protein (LBP) and fecal calprotectin	958:1049	gut-related host parameters [lipopolysaccharide-binding protein (LBP) and fecal calprotectin	958:1049	Associations between gastrointestinal transit (segmental CTT and stool frequency and consistency), microbiota diversity and composition, microbial metabolites [short-chain fatty acids (SCFA), branched-chain fatty acids, and breath hydrogen], habitual diet, and gut-related host parameters [lipopolysaccharide-binding protein (LBP) and fecal calprotectin] were investigated using univariate and multivariate approaches.
31869241	2	111	from	metabolites	373:383	arg1	study					440:444	a human cross-sectional study	416:444	a human cross-sectional study	416:444	Here, we investigate to what extent quantitative measures of (segmental) colonic transit time were related to gut microbiota composition, microbial metabolites, and gut-related parameters in a human cross-sectional study.
31869241	10	112	with	humans	1929:1934	arg1	transit					1956:1962	a long colonic transit	1941:1962	a long colonic transit	1941:1962	This study suggests that the distal colon transit may affect not only microbiota diversity but also microbial metabolism.NEW & NOTEWORTHY We extend previous findings showing that long distal colonic transit time influences microbial diversification and fermentation, whereas stool consistency is related to microbiota composition in humans with a long colonic transit.
31869241	2	113	theme	transit	306:312	arg1	time					314:317	(segmental) colonic transit time	286:317	(segmental) colonic transit time	286:317	Here, we investigate to what extent quantitative measures of (segmental) colonic transit time were related to gut microbiota composition, microbial metabolites, and gut-related parameters in a human cross-sectional study.
31869241	10	114	from	composition	1914:1924	arg1	humans					1929:1934	humans	1929:1934	humans with a long colonic transit	1929:1962	This study suggests that the distal colon transit may affect not only microbiota diversity but also microbial metabolism.NEW & NOTEWORTHY We extend previous findings showing that long distal colonic transit time influences microbial diversification and fermentation, whereas stool consistency is related to microbiota composition in humans with a long colonic transit.
31869241	9	115	theme	colonic	1503:1509	arg1	transit					1511:1517	Rectosigmoid and descending colonic transit	1475:1517	Rectosigmoid and descending colonic transit	1475:1517	Rectosigmoid and descending colonic transit were negatively associated with fecal SCFA and plasma acetate, respectively.
31869241	7	116	theme	UniFrac	1277:1283	arg1	distance					1285:1292	unweighted and weighted UniFrac distance	1253:1292	distance	1285:1292	Using unweighted and weighted UniFrac distance, microbiota variation was not related to (segmental) CTT but to demographics, diet, plasma LBP, and fecal calprotectin.
31869241	6	117	dep	distal	1139:1144	arg1	i.e.					1133:1136	i.e.	1133:1136	i.e.	1133:1136	Long descending (i.e., distal) colonic transit was associated with increased microbial α-diversity but not with stool consistency.
31869241	2	118	theme	microbiota	339:348	arg1	composition					350:360	gut microbiota composition	335:360	gut microbiota composition	335:360	Here, we investigate to what extent quantitative measures of (segmental) colonic transit time were related to gut microbiota composition, microbial metabolites, and gut-related parameters in a human cross-sectional study.
30548870	9	0	theme	wide	1069:1072	arg1	range					1074:1078	a wide range	1067:1078	a wide range of pH (6-10) and temperature (40-60 °C) values	1067:1125	It is stable in a wide range of pH (6-10) and temperature (40-60 °C) values.
30548870	4	1	theme	enzyme	330:335	arg1	β-CGTase					337:344	The enzyme β-CGTase	326:344	The enzyme β-CGTase produced from isolate ND1	326:370	The enzyme β-CGTase produced from isolate ND1 catalyzes production of β-cyclodextrin utilizing starch as a substrate which has diverse applications in various fields.
30548870	7	2	theme	specific	883:890	arg1	63.53 U mg-1					940:951	63.53 U mg-1	940:951	63.53 U mg-1	940:951	The specific activity of the purified enzyme was found to be 63.53 U mg-1 .
30548870	7	2	theme	specific	883:890	arg1	activity					892:899	The specific activity	879:899	The specific activity of the purified enzyme	879:922	The specific activity of the purified enzyme was found to be 63.53 U mg-1 .
30548870	3	3	dep	biochemical	192:202	arg1	physiologicaland					205:220	physiologicaland	205:220	physiologicaland	205:220	The biochemical, physiologicaland phylogenetic analyses based on 16S rRNA gene suggest that the isolate belongs to Bacillus cereus group.
30548870	12	4	theme	wide	1373:1376	arg1	value					1389:1393	wide commercial value	1373:1393	wide commercial value	1373:1393	Together; these results suggest that the enzyme may be of wide commercial value in various industrial processes.
30548870	6	5	theme	ammonium	806:813	arg1	precipitation					824:836	ammonium sulphate precipitation	806:836	ammonium sulphate precipitation followed by ion exchange chromatography	806:876	The crude enzyme was partially purified (29-fold) using ammonium sulphate precipitation followed by ion exchange chromatography.
30548870	4	6	theme	various	477:483	arg1	fields					485:490	various fields	477:490	various fields	477:490	The enzyme β-CGTase produced from isolate ND1 catalyzes production of β-cyclodextrin utilizing starch as a substrate which has diverse applications in various fields.
30548870	2	7	theme	bacterium	120:128	arg1	ND1					130:132	A potent β-CGTase producing bacterium ND1	92:132	A potent β-CGTase producing bacterium ND1	92:132	A potent β-CGTase producing bacterium ND1 has been isolated from sugarcane field soil in India.
30548870	3	8	theme	phylogenetic	222:233	arg1	analyses					235:242	The biochemical, physiologicaland phylogenetic analyses	188:242	The biochemical, physiologicaland phylogenetic analyses based on 16S rRNA gene	188:265	The biochemical, physiologicaland phylogenetic analyses based on 16S rRNA gene suggest that the isolate belongs to Bacillus cereus group.
30548870	9	9	theme	values	1120:1125	arg1	range					1074:1078	a wide range	1067:1078	a wide range of pH (6-10) and temperature (40-60 °C) values	1067:1125	It is stable in a wide range of pH (6-10) and temperature (40-60 °C) values.
30548870	10	10	theme	CGTase	1140:1145	arg1	activity					1147:1154	The maximum CGTase activity	1128:1154	The maximum CGTase activity	1128:1154	The maximum CGTase activity was observed at pH 9 and temperature 50 °C.
30548870	2	11	theme	field	167:171	arg1	soil					173:176	sugarcane field soil	157:176	sugarcane field soil in India	157:185	A potent β-CGTase producing bacterium ND1 has been isolated from sugarcane field soil in India.
30548870	8	12	theme	97.4 kD	1016:1022	arg1	weight					1006:1011	a molecular weight	994:1011	a molecular weight of 97.4 kD	994:1022	The enzyme is monomeric in nature with a molecular weight of 97.4 kD as determined by SDS-PAGE.
30548870	5	13	theme	Response	626:633	arg1	RSM					656:658	RSM	656:658	RSM	656:658	The enzyme production parameters pH, temperature, and substrate concentration were optimized using Central Composite Design (CCD) of Response Surface Methodology (RSM) and were found to be 8.9, 30.55 °C, and 1.88%, respectively for optimal enzyme activity.
30548870	5	13	theme	Response	626:633	arg1	Methodology					643:653	Response Surface Methodology	626:653	Response Surface Methodology (RSM)	626:659	The enzyme production parameters pH, temperature, and substrate concentration were optimized using Central Composite Design (CCD) of Response Surface Methodology (RSM) and were found to be 8.9, 30.55 °C, and 1.88%, respectively for optimal enzyme activity.
30548870	10	14	theme	maximum	1132:1138	arg1	activity					1147:1154	The maximum CGTase activity	1128:1154	The maximum CGTase activity	1128:1154	The maximum CGTase activity was observed at pH 9 and temperature 50 °C.
30548870	2	15	theme	sugarcane	157:165	arg1	soil					173:176	sugarcane field soil	157:176	sugarcane field soil in India	157:185	A potent β-CGTase producing bacterium ND1 has been isolated from sugarcane field soil in India.
30548870	5	16	theme	Methodology	643:653	arg1	CCD					618:620	CCD	618:620	CCD	618:620	The enzyme production parameters pH, temperature, and substrate concentration were optimized using Central Composite Design (CCD) of Response Surface Methodology (RSM) and were found to be 8.9, 30.55 °C, and 1.88%, respectively for optimal enzyme activity.
30548870	5	16	theme	Methodology	643:653	arg1	Design					610:615	Central Composite Design	592:615	Central Composite Design (CCD) of Response Surface Methodology (RSM)	592:659	The enzyme production parameters pH, temperature, and substrate concentration were optimized using Central Composite Design (CCD) of Response Surface Methodology (RSM) and were found to be 8.9, 30.55 °C, and 1.88%, respectively for optimal enzyme activity.
30548870	5	17	theme	Surface	635:641	arg1	RSM					656:658	RSM	656:658	RSM	656:658	The enzyme production parameters pH, temperature, and substrate concentration were optimized using Central Composite Design (CCD) of Response Surface Methodology (RSM) and were found to be 8.9, 30.55 °C, and 1.88%, respectively for optimal enzyme activity.
30548870	5	17	theme	Surface	635:641	arg1	Methodology					643:653	Response Surface Methodology	626:653	Response Surface Methodology (RSM)	626:659	The enzyme production parameters pH, temperature, and substrate concentration were optimized using Central Composite Design (CCD) of Response Surface Methodology (RSM) and were found to be 8.9, 30.55 °C, and 1.88%, respectively for optimal enzyme activity.
30548870	4	18	contain	has	449:451	arg1	starch					421:426	starch	421:426	starch	421:426	The enzyme β-CGTase produced from isolate ND1 catalyzes production of β-cyclodextrin utilizing starch as a substrate which has diverse applications in various fields.
30548870	4	18	contain	has	449:451	arg1	substrate					433:441	a substrate	431:441	a substrate which has diverse applications in various fields	431:490	The enzyme β-CGTase produced from isolate ND1 catalyzes production of β-cyclodextrin utilizing starch as a substrate which has diverse applications in various fields.
30548870	4	18	contain	has	449:451	arg2	applications					461:472	diverse applications	453:472	diverse applications	453:472	The enzyme β-CGTase produced from isolate ND1 catalyzes production of β-cyclodextrin utilizing starch as a substrate which has diverse applications in various fields.
30548870	5	19	theme	optimal	725:731	arg1	activity					740:747	optimal enzyme activity	725:747	optimal enzyme activity	725:747	The enzyme production parameters pH, temperature, and substrate concentration were optimized using Central Composite Design (CCD) of Response Surface Methodology (RSM) and were found to be 8.9, 30.55 °C, and 1.88%, respectively for optimal enzyme activity.
30548870	11	20	theme	substrate	1292:1300	arg1	specificity					1302:1312	high substrate specificity	1287:1312	high substrate specificity	1287:1312	The Km value was found to be 2.613 ± 0.5 and Vmax was 0.309 ± 0.05 µg min-1 indicating high substrate specificity.
30548870	5	21	theme	Composite	600:608	arg1	CCD					618:620	CCD	618:620	CCD	618:620	The enzyme production parameters pH, temperature, and substrate concentration were optimized using Central Composite Design (CCD) of Response Surface Methodology (RSM) and were found to be 8.9, 30.55 °C, and 1.88%, respectively for optimal enzyme activity.
30548870	5	21	theme	Composite	600:608	arg1	Design					610:615	Central Composite Design	592:615	Central Composite Design (CCD) of Response Surface Methodology (RSM)	592:659	The enzyme production parameters pH, temperature, and substrate concentration were optimized using Central Composite Design (CCD) of Response Surface Methodology (RSM) and were found to be 8.9, 30.55 °C, and 1.88%, respectively for optimal enzyme activity.
30548870	3	22	theme	16S	253:255	arg1	gene					262:265	16S rRNA gene	253:265	16S rRNA gene	253:265	The biochemical, physiologicaland phylogenetic analyses based on 16S rRNA gene suggest that the isolate belongs to Bacillus cereus group.
30548870	10	23	located	observed	1160:1167	arg1	pH					1172:1173	pH 9	1172:1175	pH 9	1172:1175	The maximum CGTase activity was observed at pH 9 and temperature 50 °C.
30548870	10	23	located	observed	1160:1167	arg1	temperature					1181:1191	temperature 50 °C	1181:1197	temperature 50 °C	1181:1197	The maximum CGTase activity was observed at pH 9 and temperature 50 °C.
30548870	10	23	located	observed	1160:1167	arg2	activity					1147:1154	The maximum CGTase activity	1128:1154	The maximum CGTase activity	1128:1154	The maximum CGTase activity was observed at pH 9 and temperature 50 °C.
30548870	3	24	theme	rRNA	257:260	arg1	gene					262:265	16S rRNA gene	253:265	16S rRNA gene	253:265	The biochemical, physiologicaland phylogenetic analyses based on 16S rRNA gene suggest that the isolate belongs to Bacillus cereus group.
30548870	4	25	theme	β-cyclodextrin	396:409	arg1	production					382:391	production	382:391	production of β-cyclodextrin utilizing starch as a substrate which has diverse applications in various fields	382:490	The enzyme β-CGTase produced from isolate ND1 catalyzes production of β-cyclodextrin utilizing starch as a substrate which has diverse applications in various fields.
30548870	11	26	theme	Km	1204:1205	arg1	value					1207:1211	The Km value	1200:1211	The Km value	1200:1211	The Km value was found to be 2.613 ± 0.5 and Vmax was 0.309 ± 0.05 µg min-1 indicating high substrate specificity.
30548870	11	26	theme	Km	1204:1205	arg1	2.613 ± 0.5					1229:1239	2.613 ± 0.5	1229:1239	2.613 ± 0.5	1229:1239	The Km value was found to be 2.613 ± 0.5 and Vmax was 0.309 ± 0.05 µg min-1 indicating high substrate specificity.
30548870	8	27	theme	molecular	996:1004	arg1	weight					1006:1011	a molecular weight	994:1011	a molecular weight of 97.4 kD	994:1022	The enzyme is monomeric in nature with a molecular weight of 97.4 kD as determined by SDS-PAGE.
30548870	7	28	theme	enzyme	917:922	arg1	63.53 U mg-1					940:951	63.53 U mg-1	940:951	63.53 U mg-1	940:951	The specific activity of the purified enzyme was found to be 63.53 U mg-1 .
30548870	7	28	theme	enzyme	917:922	arg1	activity					892:899	The specific activity	879:899	The specific activity of the purified enzyme	879:922	The specific activity of the purified enzyme was found to be 63.53 U mg-1 .
30548870	3	29	theme	biochemical	192:202	arg1	analyses					235:242	The biochemical, physiologicaland phylogenetic analyses	188:242	The biochemical, physiologicaland phylogenetic analyses based on 16S rRNA gene	188:265	The biochemical, physiologicaland phylogenetic analyses based on 16S rRNA gene suggest that the isolate belongs to Bacillus cereus group.
30548870	6	30	dep	purified	781:788	arg1	29-fold					791:797	29-fold	791:797	29-fold	791:797	The crude enzyme was partially purified (29-fold) using ammonium sulphate precipitation followed by ion exchange chromatography.
30548870	6	31	theme	exchange	854:861	arg1	chromatography					863:876	ion exchange chromatography	850:876	ion exchange chromatography	850:876	The crude enzyme was partially purified (29-fold) using ammonium sulphate precipitation followed by ion exchange chromatography.
30548870	8	32	from	nature	982:987	arg1	monomeric					969:977	monomeric	969:977	monomeric	969:977	The enzyme is monomeric in nature with a molecular weight of 97.4 kD as determined by SDS-PAGE.
30548870	8	32	from	nature	982:987	arg1	enzyme					959:964	The enzyme	955:964	The enzyme	955:964	The enzyme is monomeric in nature with a molecular weight of 97.4 kD as determined by SDS-PAGE.
30548870	5	33	theme	enzyme	497:502	arg1	%					705:705	8.9, 30.55 °C, and 1.88%	682:705	8.9, 30.55 °C, and 1.88%	682:705	The enzyme production parameters pH, temperature, and substrate concentration were optimized using Central Composite Design (CCD) of Response Surface Methodology (RSM) and were found to be 8.9, 30.55 °C, and 1.88%, respectively for optimal enzyme activity.
30548870	5	33	theme	enzyme	497:502	arg1	pH					526:527	pH	526:527	pH	526:527	The enzyme production parameters pH, temperature, and substrate concentration were optimized using Central Composite Design (CCD) of Response Surface Methodology (RSM) and were found to be 8.9, 30.55 °C, and 1.88%, respectively for optimal enzyme activity.
30548870	5	33	theme	enzyme	497:502	arg1	concentration					557:569	substrate concentration	547:569	substrate concentration	547:569	The enzyme production parameters pH, temperature, and substrate concentration were optimized using Central Composite Design (CCD) of Response Surface Methodology (RSM) and were found to be 8.9, 30.55 °C, and 1.88%, respectively for optimal enzyme activity.
30548870	5	33	theme	enzyme	497:502	arg1	parameters					515:524	The enzyme production parameters pH, temperature, and substrate concentration	493:569	The enzyme production parameters pH, temperature, and substrate concentration	493:569	The enzyme production parameters pH, temperature, and substrate concentration were optimized using Central Composite Design (CCD) of Response Surface Methodology (RSM) and were found to be 8.9, 30.55 °C, and 1.88%, respectively for optimal enzyme activity.
30548870	5	33	theme	enzyme	497:502	arg1	temperature					530:540	temperature	530:540	temperature	530:540	The enzyme production parameters pH, temperature, and substrate concentration were optimized using Central Composite Design (CCD) of Response Surface Methodology (RSM) and were found to be 8.9, 30.55 °C, and 1.88%, respectively for optimal enzyme activity.
30548870	0	34	theme	glucanotransferase	50:67	arg1	characterization					15:30	characterization	15:30	characterization	15:30	Production and characterization of β-cyclodextrin glucanotransferase from Bacillus sp.
30548870	0	34	theme	glucanotransferase	50:67	arg1	Production					0:9	Production	0:9	Production	0:9	Production and characterization of β-cyclodextrin glucanotransferase from Bacillus sp.
30548870	6	35	theme	ion	850:852	arg1	chromatography					863:876	ion exchange chromatography	850:876	ion exchange chromatography	850:876	The crude enzyme was partially purified (29-fold) using ammonium sulphate precipitation followed by ion exchange chromatography.
30548870	9	36	from	range	1074:1078	arg1	stable					1057:1062	stable	1057:1062	stable	1057:1062	It is stable in a wide range of pH (6-10) and temperature (40-60 °C) values.
30548870	5	37	theme	production	504:513	arg1	%					705:705	8.9, 30.55 °C, and 1.88%	682:705	8.9, 30.55 °C, and 1.88%	682:705	The enzyme production parameters pH, temperature, and substrate concentration were optimized using Central Composite Design (CCD) of Response Surface Methodology (RSM) and were found to be 8.9, 30.55 °C, and 1.88%, respectively for optimal enzyme activity.
30548870	5	37	theme	production	504:513	arg1	pH					526:527	pH	526:527	pH	526:527	The enzyme production parameters pH, temperature, and substrate concentration were optimized using Central Composite Design (CCD) of Response Surface Methodology (RSM) and were found to be 8.9, 30.55 °C, and 1.88%, respectively for optimal enzyme activity.
30548870	5	37	theme	production	504:513	arg1	concentration					557:569	substrate concentration	547:569	substrate concentration	547:569	The enzyme production parameters pH, temperature, and substrate concentration were optimized using Central Composite Design (CCD) of Response Surface Methodology (RSM) and were found to be 8.9, 30.55 °C, and 1.88%, respectively for optimal enzyme activity.
30548870	5	37	theme	production	504:513	arg1	parameters					515:524	The enzyme production parameters pH, temperature, and substrate concentration	493:569	The enzyme production parameters pH, temperature, and substrate concentration	493:569	The enzyme production parameters pH, temperature, and substrate concentration were optimized using Central Composite Design (CCD) of Response Surface Methodology (RSM) and were found to be 8.9, 30.55 °C, and 1.88%, respectively for optimal enzyme activity.
30548870	5	37	theme	production	504:513	arg1	temperature					530:540	temperature	530:540	temperature	530:540	The enzyme production parameters pH, temperature, and substrate concentration were optimized using Central Composite Design (CCD) of Response Surface Methodology (RSM) and were found to be 8.9, 30.55 °C, and 1.88%, respectively for optimal enzyme activity.
30548870	0	38	theme	β-cyclodextrin	35:48	arg1	glucanotransferase					50:67	β-cyclodextrin glucanotransferase	35:67	β-cyclodextrin glucanotransferase	35:67	Production and characterization of β-cyclodextrin glucanotransferase from Bacillus sp.
30548870	2	39	theme	producing	110:118	arg1	ND1					130:132	A potent β-CGTase producing bacterium ND1	92:132	A potent β-CGTase producing bacterium ND1	92:132	A potent β-CGTase producing bacterium ND1 has been isolated from sugarcane field soil in India.
30548870	9	40	theme	pH	1083:1084	arg1	values					1120:1125	pH (6-10) and temperature (40-60 °C) values	1083:1125	pH (6-10) and temperature (40-60 °C) values	1083:1125	It is stable in a wide range of pH (6-10) and temperature (40-60 °C) values.
30548870	0	41	theme	Bacillus	74:81	arg1	sp					83:84	Bacillus sp	74:84	Bacillus sp	74:84	Production and characterization of β-cyclodextrin glucanotransferase from Bacillus sp.
30548870	2	42	theme	β-CGTase	101:108	arg1	ND1					130:132	A potent β-CGTase producing bacterium ND1	92:132	A potent β-CGTase producing bacterium ND1	92:132	A potent β-CGTase producing bacterium ND1 has been isolated from sugarcane field soil in India.
30548870	3	43	theme	cereus	312:317	arg1	group					319:323	Bacillus cereus group	303:323	Bacillus cereus group	303:323	The biochemical, physiologicaland phylogenetic analyses based on 16S rRNA gene suggest that the isolate belongs to Bacillus cereus group.
30548870	0	44	from	sp	83:84	arg1	characterization					15:30	characterization	15:30	characterization	15:30	Production and characterization of β-cyclodextrin glucanotransferase from Bacillus sp.
30548870	0	44	from	sp	83:84	arg1	Production					0:9	Production	0:9	Production	0:9	Production and characterization of β-cyclodextrin glucanotransferase from Bacillus sp.
30548870	6	45	theme	crude	754:758	arg1	enzyme					760:765	The crude enzyme	750:765	The crude enzyme	750:765	The crude enzyme was partially purified (29-fold) using ammonium sulphate precipitation followed by ion exchange chromatography.
30548870	11	46	theme	high	1287:1290	arg1	specificity					1302:1312	high substrate specificity	1287:1312	high substrate specificity	1287:1312	The Km value was found to be 2.613 ± 0.5 and Vmax was 0.309 ± 0.05 µg min-1 indicating high substrate specificity.
30548870	2	47	theme	potent	94:99	arg1	ND1					130:132	A potent β-CGTase producing bacterium ND1	92:132	A potent β-CGTase producing bacterium ND1	92:132	A potent β-CGTase producing bacterium ND1 has been isolated from sugarcane field soil in India.
30548870	12	48	theme	industrial	1406:1415	arg1	processes					1417:1425	various industrial processes	1398:1425	various industrial processes	1398:1425	Together; these results suggest that the enzyme may be of wide commercial value in various industrial processes.
30548870	12	49	theme	various	1398:1404	arg1	processes					1417:1425	various industrial processes	1398:1425	various industrial processes	1398:1425	Together; these results suggest that the enzyme may be of wide commercial value in various industrial processes.
30548870	5	50	dep	parameters	515:524	arg1	pH					526:527	pH	526:527	pH	526:527	The enzyme production parameters pH, temperature, and substrate concentration were optimized using Central Composite Design (CCD) of Response Surface Methodology (RSM) and were found to be 8.9, 30.55 °C, and 1.88%, respectively for optimal enzyme activity.
30548870	5	50	dep	parameters	515:524	arg1	concentration					557:569	substrate concentration	547:569	substrate concentration	547:569	The enzyme production parameters pH, temperature, and substrate concentration were optimized using Central Composite Design (CCD) of Response Surface Methodology (RSM) and were found to be 8.9, 30.55 °C, and 1.88%, respectively for optimal enzyme activity.
30548870	5	50	dep	parameters	515:524	arg1	parameters					515:524	The enzyme production parameters pH, temperature, and substrate concentration	493:569	The enzyme production parameters pH, temperature, and substrate concentration	493:569	The enzyme production parameters pH, temperature, and substrate concentration were optimized using Central Composite Design (CCD) of Response Surface Methodology (RSM) and were found to be 8.9, 30.55 °C, and 1.88%, respectively for optimal enzyme activity.
30548870	5	50	dep	parameters	515:524	arg1	temperature					530:540	temperature	530:540	temperature	530:540	The enzyme production parameters pH, temperature, and substrate concentration were optimized using Central Composite Design (CCD) of Response Surface Methodology (RSM) and were found to be 8.9, 30.55 °C, and 1.88%, respectively for optimal enzyme activity.
30548870	8	51	from	monomeric	969:977	arg1	nature					982:987	nature	982:987	nature	982:987	The enzyme is monomeric in nature with a molecular weight of 97.4 kD as determined by SDS-PAGE.
30548870	5	52	theme	Central	592:598	arg1	CCD					618:620	CCD	618:620	CCD	618:620	The enzyme production parameters pH, temperature, and substrate concentration were optimized using Central Composite Design (CCD) of Response Surface Methodology (RSM) and were found to be 8.9, 30.55 °C, and 1.88%, respectively for optimal enzyme activity.
30548870	5	52	theme	Central	592:598	arg1	Design					610:615	Central Composite Design	592:615	Central Composite Design (CCD) of Response Surface Methodology (RSM)	592:659	The enzyme production parameters pH, temperature, and substrate concentration were optimized using Central Composite Design (CCD) of Response Surface Methodology (RSM) and were found to be 8.9, 30.55 °C, and 1.88%, respectively for optimal enzyme activity.
30548870	6	53	theme	sulphate	815:822	arg1	precipitation					824:836	ammonium sulphate precipitation	806:836	ammonium sulphate precipitation followed by ion exchange chromatography	806:876	The crude enzyme was partially purified (29-fold) using ammonium sulphate precipitation followed by ion exchange chromatography.
30548870	9	54	from	stable	1057:1062	arg1	range					1074:1078	a wide range	1067:1078	a wide range of pH (6-10) and temperature (40-60 °C) values	1067:1125	It is stable in a wide range of pH (6-10) and temperature (40-60 °C) values.
30548870	4	55	theme	isolate	360:366	arg1	ND1					368:370	isolate ND1	360:370	isolate ND1	360:370	The enzyme β-CGTase produced from isolate ND1 catalyzes production of β-cyclodextrin utilizing starch as a substrate which has diverse applications in various fields.
30548870	2	56	attach	isolated	143:150	arg1	soil					173:176	sugarcane field soil	157:176	sugarcane field soil in India	157:185	A potent β-CGTase producing bacterium ND1 has been isolated from sugarcane field soil in India.
30548870	2	56	attach	isolated	143:150	arg2	ND1					130:132	A potent β-CGTase producing bacterium ND1	92:132	A potent β-CGTase producing bacterium ND1	92:132	A potent β-CGTase producing bacterium ND1 has been isolated from sugarcane field soil in India.
30548870	7	57	theme	purified	908:915	arg1	enzyme					917:922	the purified enzyme	904:922	the purified enzyme	904:922	The specific activity of the purified enzyme was found to be 63.53 U mg-1 .
30548870	3	58	theme	Bacillus	303:310	arg1	group					319:323	Bacillus cereus group	303:323	Bacillus cereus group	303:323	The biochemical, physiologicaland phylogenetic analyses based on 16S rRNA gene suggest that the isolate belongs to Bacillus cereus group.
30548870	4	59	theme	diverse	453:459	arg1	applications					461:472	diverse applications	453:472	diverse applications	453:472	The enzyme β-CGTase produced from isolate ND1 catalyzes production of β-cyclodextrin utilizing starch as a substrate which has diverse applications in various fields.
30548870	8	60	with	monomeric	969:977	arg1	weight					1006:1011	a molecular weight	994:1011	a molecular weight of 97.4 kD	994:1022	The enzyme is monomeric in nature with a molecular weight of 97.4 kD as determined by SDS-PAGE.
30548870	5	61	theme	enzyme	733:738	arg1	activity					740:747	optimal enzyme activity	725:747	optimal enzyme activity	725:747	The enzyme production parameters pH, temperature, and substrate concentration were optimized using Central Composite Design (CCD) of Response Surface Methodology (RSM) and were found to be 8.9, 30.55 °C, and 1.88%, respectively for optimal enzyme activity.
30548870	9	62	theme	temperature	1097:1107	arg1	values					1120:1125	pH (6-10) and temperature (40-60 °C) values	1083:1125	pH (6-10) and temperature (40-60 °C) values	1083:1125	It is stable in a wide range of pH (6-10) and temperature (40-60 °C) values.
30548870	2	63	from	soil	173:176	arg1	India					181:185	India	181:185	India	181:185	A potent β-CGTase producing bacterium ND1 has been isolated from sugarcane field soil in India.
30548870	12	64	theme	commercial	1378:1387	arg1	value					1389:1393	wide commercial value	1373:1393	wide commercial value	1373:1393	Together; these results suggest that the enzyme may be of wide commercial value in various industrial processes.
30548870	5	65	theme	substrate	547:555	arg1	concentration					557:569	substrate concentration	547:569	substrate concentration	547:569	The enzyme production parameters pH, temperature, and substrate concentration were optimized using Central Composite Design (CCD) of Response Surface Methodology (RSM) and were found to be 8.9, 30.55 °C, and 1.88%, respectively for optimal enzyme activity.
30548870	5	65	theme	substrate	547:555	arg1	parameters					515:524	The enzyme production parameters pH, temperature, and substrate concentration	493:569	The enzyme production parameters pH, temperature, and substrate concentration	493:569	The enzyme production parameters pH, temperature, and substrate concentration were optimized using Central Composite Design (CCD) of Response Surface Methodology (RSM) and were found to be 8.9, 30.55 °C, and 1.88%, respectively for optimal enzyme activity.
30548870	12	66	from	value	1389:1393	arg1	processes					1417:1425	various industrial processes	1398:1425	various industrial processes	1398:1425	Together; these results suggest that the enzyme may be of wide commercial value in various industrial processes.
31874680	0	0	theme	steam-treated	81:93	arg1	bagasse					105:111	steam-treated sugarcane bagasse	81:111	steam-treated sugarcane bagasse	81:111	The boosting effect of recombinant hemicellulases on the enzymatic hydrolysis of steam-treated sugarcane bagasse.
31874680	4	1	theme	enzyme	980:985	arg1	mixture					987:993	an efficient enzyme mixture	967:993	an efficient enzyme mixture	967:993	One recombinant endoxylanase (HXYN2) and one recombinant β-xylosidase (HXYLA) from Humicola grisea var thermoidea, together with an α-l-arabinofuranosidase (AFB3) from Penicillium pupurogenum, all produced in Pichia pastoris, were used to formulate an efficient enzyme mixture for SEB hydrolysis using a 23 Central Composite Rotatable Design (CCRD).
31874680	6	2	theme	sequential	1260:1269	arg1	experiments					1271:1281	sequential experiments	1260:1281	sequential experiments	1260:1281	Subsequently, the optimal enzyme mixture was used in combination with commercial cellulases (Accellerase 1500), either simultaneously or in sequential experiments.
31874680	9	3	theme	enzymatic	1723:1731	arg1	hydrolysis					1733:1742	enzymatic hydrolysis	1723:1742	enzymatic hydrolysis of SEB	1723:1749	Our results show the potential of complementary enzyme activities to improve enzymatic hydrolysis of SEB, thus improving the efficiency of the hydrolytic process.
31874680	4	4	theme	Composite	1033:1041	arg1	Design					1053:1058	a 23 Central Composite Rotatable Design	1020:1058	a 23 Central Composite Rotatable Design (CCRD)	1020:1065	One recombinant endoxylanase (HXYN2) and one recombinant β-xylosidase (HXYLA) from Humicola grisea var thermoidea, together with an α-l-arabinofuranosidase (AFB3) from Penicillium pupurogenum, all produced in Pichia pastoris, were used to formulate an efficient enzyme mixture for SEB hydrolysis using a 23 Central Composite Rotatable Design (CCRD).
31874680	4	4	theme	Composite	1033:1041	arg1	CCRD					1061:1064	CCRD	1061:1064	CCRD	1061:1064	One recombinant endoxylanase (HXYN2) and one recombinant β-xylosidase (HXYLA) from Humicola grisea var thermoidea, together with an α-l-arabinofuranosidase (AFB3) from Penicillium pupurogenum, all produced in Pichia pastoris, were used to formulate an efficient enzyme mixture for SEB hydrolysis using a 23 Central Composite Rotatable Design (CCRD).
31874680	6	5	dep	either	1232:1237	arg1	simultaneously					1239:1252	simultaneously	1239:1252	simultaneously	1239:1252	Subsequently, the optimal enzyme mixture was used in combination with commercial cellulases (Accellerase 1500), either simultaneously or in sequential experiments.
31874680	4	6	theme	efficient	970:978	arg1	mixture					987:993	an efficient enzyme mixture	967:993	an efficient enzyme mixture	967:993	One recombinant endoxylanase (HXYN2) and one recombinant β-xylosidase (HXYLA) from Humicola grisea var thermoidea, together with an α-l-arabinofuranosidase (AFB3) from Penicillium pupurogenum, all produced in Pichia pastoris, were used to formulate an efficient enzyme mixture for SEB hydrolysis using a 23 Central Composite Rotatable Design (CCRD).
31874680	3	7	theme	bagasse	703:709	arg1	hydrolysis					664:673	the enzymatic hydrolysis	650:673	the enzymatic hydrolysis of steam-exploded sugarcane bagasse (SEB)	650:715	The current work aimed at evaluating the effect of recombinant hemicellulolytic enzymes on the enzymatic hydrolysis of steam-exploded sugarcane bagasse (SEB).
31874680	9	8	theme	SEB	1747:1749	arg1	hydrolysis					1733:1742	enzymatic hydrolysis	1723:1742	enzymatic hydrolysis of SEB	1723:1749	Our results show the potential of complementary enzyme activities to improve enzymatic hydrolysis of SEB, thus improving the efficiency of the hydrolytic process.
31874680	2	9	theme	crops	360:364	arg1	component					340:348	the main hemicellulose component	317:348	the main hemicellulose component of energy crops	317:364	Xylan is the main hemicellulose component of energy crops and for its complete hydrolysis a system consisting of several enzymes acting cooperatively, including endoxylanases (XYN), β-xylosidases (XYL) and α-l-arabinofuranosidases (ABF) is necessary.
31874680	2	9	theme	crops	360:364	arg1	Xylan					308:312	Xylan	308:312	Xylan	308:312	Xylan is the main hemicellulose component of energy crops and for its complete hydrolysis a system consisting of several enzymes acting cooperatively, including endoxylanases (XYN), β-xylosidases (XYL) and α-l-arabinofuranosidases (ABF) is necessary.
31874680	1	10	theme	enzymes	257:263	arg1	combination					242:252	a combination	240:252	a combination of enzymes with complementary activities	240:293	To increase the efficiency of enzyme cocktails in deconstructing cellulose and hemicelluloses present in the plant cell wall, a combination of enzymes with complementary activities is required.
31874680	0	11	theme	bagasse	105:111	arg1	hydrolysis					67:76	the enzymatic hydrolysis	53:76	the enzymatic hydrolysis of steam-treated sugarcane bagasse	53:111	The boosting effect of recombinant hemicellulases on the enzymatic hydrolysis of steam-treated sugarcane bagasse.
31874680	7	12	theme	glucose	1357:1363	arg1	yield					1365:1369	the glucose yield	1353:1369	the glucose yield from SEB hydrolysis	1353:1389	The supplementation of Accellerase 1500 with hemicellulases enhanced the glucose yield from SEB hydrolysis by 14.6%, but this effect could be raised to 50% when hemicellulases were added prior to hydrolysis with commercial cellulases.
31874680	4	13	theme	recombinant	763:773	arg1	HXYLA					789:793	HXYLA	789:793	HXYLA	789:793	One recombinant endoxylanase (HXYN2) and one recombinant β-xylosidase (HXYLA) from Humicola grisea var thermoidea, together with an α-l-arabinofuranosidase (AFB3) from Penicillium pupurogenum, all produced in Pichia pastoris, were used to formulate an efficient enzyme mixture for SEB hydrolysis using a 23 Central Composite Rotatable Design (CCRD).
31874680	4	13	theme	recombinant	763:773	arg1	β-xylosidase					775:786	one recombinant β-xylosidase	759:786	one recombinant β-xylosidase (HXYLA)	759:794	One recombinant endoxylanase (HXYN2) and one recombinant β-xylosidase (HXYLA) from Humicola grisea var thermoidea, together with an α-l-arabinofuranosidase (AFB3) from Penicillium pupurogenum, all produced in Pichia pastoris, were used to formulate an efficient enzyme mixture for SEB hydrolysis using a 23 Central Composite Rotatable Design (CCRD).
31874680	8	14	theme	hydrolysis	1620:1629	arg1	effect					1600:1605	the effect	1596:1605	the effect of enzymatic hydrolysis on SEB fibers	1596:1643	These results were supported by scanning electron microscopy, which revealed the effect of enzymatic hydrolysis on SEB fibers.
31874680	0	15	theme	sugarcane	95:103	arg1	bagasse					105:111	steam-treated sugarcane bagasse	81:111	steam-treated sugarcane bagasse	81:111	The boosting effect of recombinant hemicellulases on the enzymatic hydrolysis of steam-treated sugarcane bagasse.
31874680	4	16	from	α-l-arabinofuranosidase	850:872	arg1	HXYLA					789:793	HXYLA	789:793	HXYLA	789:793	One recombinant endoxylanase (HXYN2) and one recombinant β-xylosidase (HXYLA) from Humicola grisea var thermoidea, together with an α-l-arabinofuranosidase (AFB3) from Penicillium pupurogenum, all produced in Pichia pastoris, were used to formulate an efficient enzyme mixture for SEB hydrolysis using a 23 Central Composite Rotatable Design (CCRD).
31874680	4	16	from	α-l-arabinofuranosidase	850:872	arg1	β-xylosidase					775:786	one recombinant β-xylosidase	759:786	one recombinant β-xylosidase (HXYLA)	759:794	One recombinant endoxylanase (HXYN2) and one recombinant β-xylosidase (HXYLA) from Humicola grisea var thermoidea, together with an α-l-arabinofuranosidase (AFB3) from Penicillium pupurogenum, all produced in Pichia pastoris, were used to formulate an efficient enzyme mixture for SEB hydrolysis using a 23 Central Composite Rotatable Design (CCRD).
31874680	4	16	from	α-l-arabinofuranosidase	850:872	arg1	HXYN2					748:752	HXYN2	748:752	HXYN2	748:752	One recombinant endoxylanase (HXYN2) and one recombinant β-xylosidase (HXYLA) from Humicola grisea var thermoidea, together with an α-l-arabinofuranosidase (AFB3) from Penicillium pupurogenum, all produced in Pichia pastoris, were used to formulate an efficient enzyme mixture for SEB hydrolysis using a 23 Central Composite Rotatable Design (CCRD).
31874680	4	16	from	α-l-arabinofuranosidase	850:872	arg1	endoxylanase					734:745	One recombinant endoxylanase	718:745	One recombinant endoxylanase (HXYN2)	718:753	One recombinant endoxylanase (HXYN2) and one recombinant β-xylosidase (HXYLA) from Humicola grisea var thermoidea, together with an α-l-arabinofuranosidase (AFB3) from Penicillium pupurogenum, all produced in Pichia pastoris, were used to formulate an efficient enzyme mixture for SEB hydrolysis using a 23 Central Composite Rotatable Design (CCRD).
31874680	8	17	theme	enzymatic	1610:1618	arg1	hydrolysis					1620:1629	enzymatic hydrolysis	1610:1629	enzymatic hydrolysis	1610:1629	These results were supported by scanning electron microscopy, which revealed the effect of enzymatic hydrolysis on SEB fibers.
31874680	3	18	from	effect	600:605	arg1	hydrolysis					664:673	the enzymatic hydrolysis	650:673	the enzymatic hydrolysis of steam-exploded sugarcane bagasse (SEB)	650:715	The current work aimed at evaluating the effect of recombinant hemicellulolytic enzymes on the enzymatic hydrolysis of steam-exploded sugarcane bagasse (SEB).
31874680	1	19	theme	complementary	270:282	arg1	activities					284:293	complementary activities	270:293	complementary activities	270:293	To increase the efficiency of enzyme cocktails in deconstructing cellulose and hemicelluloses present in the plant cell wall, a combination of enzymes with complementary activities is required.
31874680	5	20	theme	potent	1077:1082	arg1	enzyme					1084:1089	The most potent enzyme	1068:1089	The most potent enzyme for SEB hydrolysis	1068:1108	The most potent enzyme for SEB hydrolysis was ABF3.
31874680	5	20	theme	potent	1077:1082	arg1	ABF3					1114:1117	ABF3	1114:1117	ABF3	1114:1117	The most potent enzyme for SEB hydrolysis was ABF3.
31874680	6	21	theme	enzyme	1146:1151	arg1	mixture					1153:1159	the optimal enzyme mixture	1134:1159	the optimal enzyme mixture	1134:1159	Subsequently, the optimal enzyme mixture was used in combination with commercial cellulases (Accellerase 1500), either simultaneously or in sequential experiments.
31874680	6	22	with	combination	1173:1183	arg1	cellulases					1201:1210	commercial cellulases	1190:1210	commercial cellulases (Accellerase 1500)	1190:1229	Subsequently, the optimal enzyme mixture was used in combination with commercial cellulases (Accellerase 1500), either simultaneously or in sequential experiments.
31874680	6	22	with	combination	1173:1183	arg1	Accellerase					1213:1223	Accellerase	1213:1223	Accellerase	1213:1223	Subsequently, the optimal enzyme mixture was used in combination with commercial cellulases (Accellerase 1500), either simultaneously or in sequential experiments.
31874680	6	23	theme	optimal	1138:1144	arg1	mixture					1153:1159	the optimal enzyme mixture	1134:1159	the optimal enzyme mixture	1134:1159	Subsequently, the optimal enzyme mixture was used in combination with commercial cellulases (Accellerase 1500), either simultaneously or in sequential experiments.
31874680	0	24	theme	boosting	4:11	arg1	effect					13:18	The boosting effect	0:18	The boosting effect of recombinant	0:33	The boosting effect of recombinant hemicellulases on the enzymatic hydrolysis of steam-treated sugarcane bagasse.
31874680	2	25	theme	main	321:324	arg1	component					340:348	the main hemicellulose component	317:348	the main hemicellulose component of energy crops	317:364	Xylan is the main hemicellulose component of energy crops and for its complete hydrolysis a system consisting of several enzymes acting cooperatively, including endoxylanases (XYN), β-xylosidases (XYL) and α-l-arabinofuranosidases (ABF) is necessary.
31874680	2	25	theme	main	321:324	arg1	Xylan					308:312	Xylan	308:312	Xylan	308:312	Xylan is the main hemicellulose component of energy crops and for its complete hydrolysis a system consisting of several enzymes acting cooperatively, including endoxylanases (XYN), β-xylosidases (XYL) and α-l-arabinofuranosidases (ABF) is necessary.
31874680	7	26	with	hydrolysis	1480:1489	arg1	cellulases					1507:1516	commercial cellulases	1496:1516	commercial cellulases	1496:1516	The supplementation of Accellerase 1500 with hemicellulases enhanced the glucose yield from SEB hydrolysis by 14.6%, but this effect could be raised to 50% when hemicellulases were added prior to hydrolysis with commercial cellulases.
31874680	9	27	theme	enzyme	1694:1699	arg1	activities					1701:1710	complementary enzyme activities	1680:1710	complementary enzyme activities	1680:1710	Our results show the potential of complementary enzyme activities to improve enzymatic hydrolysis of SEB, thus improving the efficiency of the hydrolytic process.
31874680	4	28	theme	recombinant	722:732	arg1	HXYN2					748:752	HXYN2	748:752	HXYN2	748:752	One recombinant endoxylanase (HXYN2) and one recombinant β-xylosidase (HXYLA) from Humicola grisea var thermoidea, together with an α-l-arabinofuranosidase (AFB3) from Penicillium pupurogenum, all produced in Pichia pastoris, were used to formulate an efficient enzyme mixture for SEB hydrolysis using a 23 Central Composite Rotatable Design (CCRD).
31874680	4	28	theme	recombinant	722:732	arg1	endoxylanase					734:745	One recombinant endoxylanase	718:745	One recombinant endoxylanase (HXYN2)	718:753	One recombinant endoxylanase (HXYN2) and one recombinant β-xylosidase (HXYLA) from Humicola grisea var thermoidea, together with an α-l-arabinofuranosidase (AFB3) from Penicillium pupurogenum, all produced in Pichia pastoris, were used to formulate an efficient enzyme mixture for SEB hydrolysis using a 23 Central Composite Rotatable Design (CCRD).
31874680	4	29	used	used	949:952	arg2	endoxylanase					734:745	One recombinant endoxylanase	718:745	One recombinant endoxylanase (HXYN2)	718:753	One recombinant endoxylanase (HXYN2) and one recombinant β-xylosidase (HXYLA) from Humicola grisea var thermoidea, together with an α-l-arabinofuranosidase (AFB3) from Penicillium pupurogenum, all produced in Pichia pastoris, were used to formulate an efficient enzyme mixture for SEB hydrolysis using a 23 Central Composite Rotatable Design (CCRD).
31874680	4	29	used	used	949:952	arg2	HXYLA					789:793	HXYLA	789:793	HXYLA	789:793	One recombinant endoxylanase (HXYN2) and one recombinant β-xylosidase (HXYLA) from Humicola grisea var thermoidea, together with an α-l-arabinofuranosidase (AFB3) from Penicillium pupurogenum, all produced in Pichia pastoris, were used to formulate an efficient enzyme mixture for SEB hydrolysis using a 23 Central Composite Rotatable Design (CCRD).
31874680	4	29	used	used	949:952	arg2	β-xylosidase					775:786	one recombinant β-xylosidase	759:786	one recombinant β-xylosidase (HXYLA)	759:794	One recombinant endoxylanase (HXYN2) and one recombinant β-xylosidase (HXYLA) from Humicola grisea var thermoidea, together with an α-l-arabinofuranosidase (AFB3) from Penicillium pupurogenum, all produced in Pichia pastoris, were used to formulate an efficient enzyme mixture for SEB hydrolysis using a 23 Central Composite Rotatable Design (CCRD).
31874680	4	29	used	used	949:952	arg2	HXYN2					748:752	HXYN2	748:752	HXYN2	748:752	One recombinant endoxylanase (HXYN2) and one recombinant β-xylosidase (HXYLA) from Humicola grisea var thermoidea, together with an α-l-arabinofuranosidase (AFB3) from Penicillium pupurogenum, all produced in Pichia pastoris, were used to formulate an efficient enzyme mixture for SEB hydrolysis using a 23 Central Composite Rotatable Design (CCRD).
31874680	2	30	theme	complete	378:385	arg1	hydrolysis					387:396	its complete hydrolysis	374:396	its complete hydrolysis	374:396	Xylan is the main hemicellulose component of energy crops and for its complete hydrolysis a system consisting of several enzymes acting cooperatively, including endoxylanases (XYN), β-xylosidases (XYL) and α-l-arabinofuranosidases (ABF) is necessary.
31874680	4	31	theme	Central	1025:1031	arg1	Design					1053:1058	a 23 Central Composite Rotatable Design	1020:1058	a 23 Central Composite Rotatable Design (CCRD)	1020:1065	One recombinant endoxylanase (HXYN2) and one recombinant β-xylosidase (HXYLA) from Humicola grisea var thermoidea, together with an α-l-arabinofuranosidase (AFB3) from Penicillium pupurogenum, all produced in Pichia pastoris, were used to formulate an efficient enzyme mixture for SEB hydrolysis using a 23 Central Composite Rotatable Design (CCRD).
31874680	4	31	theme	Central	1025:1031	arg1	CCRD					1061:1064	CCRD	1061:1064	CCRD	1061:1064	One recombinant endoxylanase (HXYN2) and one recombinant β-xylosidase (HXYLA) from Humicola grisea var thermoidea, together with an α-l-arabinofuranosidase (AFB3) from Penicillium pupurogenum, all produced in Pichia pastoris, were used to formulate an efficient enzyme mixture for SEB hydrolysis using a 23 Central Composite Rotatable Design (CCRD).
31874680	9	32	theme	hydrolytic	1789:1798	arg1	process					1800:1806	the hydrolytic process	1785:1806	the hydrolytic process	1785:1806	Our results show the potential of complementary enzyme activities to improve enzymatic hydrolysis of SEB, thus improving the efficiency of the hydrolytic process.
31874680	7	33	theme	SEB	1376:1378	arg1	hydrolysis					1380:1389	SEB hydrolysis	1376:1389	SEB hydrolysis	1376:1389	The supplementation of Accellerase 1500 with hemicellulases enhanced the glucose yield from SEB hydrolysis by 14.6%, but this effect could be raised to 50% when hemicellulases were added prior to hydrolysis with commercial cellulases.
31874680	1	34	from	present	208:214	arg1	wall					234:237	the plant cell wall	219:237	the plant cell wall	219:237	To increase the efficiency of enzyme cocktails in deconstructing cellulose and hemicelluloses present in the plant cell wall, a combination of enzymes with complementary activities is required.
31874680	8	35	theme	SEB	1634:1636	arg1	fibers					1638:1643	SEB fibers	1634:1643	SEB fibers	1634:1643	These results were supported by scanning electron microscopy, which revealed the effect of enzymatic hydrolysis on SEB fibers.
31874680	7	36	from	hydrolysis	1380:1389	arg1	yield					1365:1369	the glucose yield	1353:1369	the glucose yield from SEB hydrolysis	1353:1389	The supplementation of Accellerase 1500 with hemicellulases enhanced the glucose yield from SEB hydrolysis by 14.6%, but this effect could be raised to 50% when hemicellulases were added prior to hydrolysis with commercial cellulases.
31874680	2	37	theme	several	421:427	arg1	enzymes					429:435	several enzymes	421:435	several enzymes acting cooperatively, including endoxylanases (XYN), β-xylosidases (XYL) and α-l-arabinofuranosidases (ABF)	421:543	Xylan is the main hemicellulose component of energy crops and for its complete hydrolysis a system consisting of several enzymes acting cooperatively, including endoxylanases (XYN), β-xylosidases (XYL) and α-l-arabinofuranosidases (ABF) is necessary.
31874680	8	38	theme	electron	1560:1567	arg1	microscopy					1569:1578	scanning electron microscopy	1551:1578	scanning electron microscopy	1551:1578	These results were supported by scanning electron microscopy, which revealed the effect of enzymatic hydrolysis on SEB fibers.
31874680	9	39	theme	complementary	1680:1692	arg1	activities					1701:1710	complementary enzyme activities	1680:1710	complementary enzyme activities	1680:1710	Our results show the potential of complementary enzyme activities to improve enzymatic hydrolysis of SEB, thus improving the efficiency of the hydrolytic process.
31874680	3	40	theme	enzymatic	654:662	arg1	hydrolysis					664:673	the enzymatic hydrolysis	650:673	the enzymatic hydrolysis of steam-exploded sugarcane bagasse (SEB)	650:715	The current work aimed at evaluating the effect of recombinant hemicellulolytic enzymes on the enzymatic hydrolysis of steam-exploded sugarcane bagasse (SEB).
31874680	4	41	theme	var	817:819	arg1	thermoidea					821:830	Humicola grisea var thermoidea	801:830	Humicola grisea var thermoidea	801:830	One recombinant endoxylanase (HXYN2) and one recombinant β-xylosidase (HXYLA) from Humicola grisea var thermoidea, together with an α-l-arabinofuranosidase (AFB3) from Penicillium pupurogenum, all produced in Pichia pastoris, were used to formulate an efficient enzyme mixture for SEB hydrolysis using a 23 Central Composite Rotatable Design (CCRD).
31874680	7	42	theme	Accellerase	1307:1317	arg1	supplementation					1288:1302	The supplementation	1284:1302	The supplementation of Accellerase 1500 with hemicellulases	1284:1342	The supplementation of Accellerase 1500 with hemicellulases enhanced the glucose yield from SEB hydrolysis by 14.6%, but this effect could be raised to 50% when hemicellulases were added prior to hydrolysis with commercial cellulases.
31874680	1	43	theme	plant	223:227	arg1	wall					234:237	the plant cell wall	219:237	the plant cell wall	219:237	To increase the efficiency of enzyme cocktails in deconstructing cellulose and hemicelluloses present in the plant cell wall, a combination of enzymes with complementary activities is required.
31874680	0	44	theme	recombinant	23:33	arg1	effect					13:18	The boosting effect	0:18	The boosting effect of recombinant	0:33	The boosting effect of recombinant hemicellulases on the enzymatic hydrolysis of steam-treated sugarcane bagasse.
31874680	8	45	theme	scanning	1551:1558	arg1	microscopy					1569:1578	scanning electron microscopy	1551:1578	scanning electron microscopy	1551:1578	These results were supported by scanning electron microscopy, which revealed the effect of enzymatic hydrolysis on SEB fibers.
31874680	4	46	theme	Pichia	927:932	arg1	pastoris					934:941	Pichia pastoris	927:941	Pichia pastoris	927:941	One recombinant endoxylanase (HXYN2) and one recombinant β-xylosidase (HXYLA) from Humicola grisea var thermoidea, together with an α-l-arabinofuranosidase (AFB3) from Penicillium pupurogenum, all produced in Pichia pastoris, were used to formulate an efficient enzyme mixture for SEB hydrolysis using a 23 Central Composite Rotatable Design (CCRD).
31874680	3	47	theme	enzymes	639:645	arg1	effect					600:605	the effect	596:605	the effect of recombinant hemicellulolytic enzymes on the enzymatic hydrolysis of steam-exploded sugarcane bagasse (SEB)	596:715	The current work aimed at evaluating the effect of recombinant hemicellulolytic enzymes on the enzymatic hydrolysis of steam-exploded sugarcane bagasse (SEB).
31874680	4	48	theme	grisea	810:815	arg1	thermoidea					821:830	Humicola grisea var thermoidea	801:830	Humicola grisea var thermoidea	801:830	One recombinant endoxylanase (HXYN2) and one recombinant β-xylosidase (HXYLA) from Humicola grisea var thermoidea, together with an α-l-arabinofuranosidase (AFB3) from Penicillium pupurogenum, all produced in Pichia pastoris, were used to formulate an efficient enzyme mixture for SEB hydrolysis using a 23 Central Composite Rotatable Design (CCRD).
31874680	1	49	theme	cell	229:232	arg1	wall					234:237	the plant cell wall	219:237	the plant cell wall	219:237	To increase the efficiency of enzyme cocktails in deconstructing cellulose and hemicelluloses present in the plant cell wall, a combination of enzymes with complementary activities is required.
31874680	9	50	theme	process	1800:1806	arg1	efficiency					1771:1780	the efficiency	1767:1780	the efficiency of the hydrolytic process	1767:1806	Our results show the potential of complementary enzyme activities to improve enzymatic hydrolysis of SEB, thus improving the efficiency of the hydrolytic process.
31874680	9	51	theme	activities	1701:1710	arg1	potential					1667:1675	the potential	1663:1675	the potential of complementary enzyme activities to improve enzymatic hydrolysis of SEB	1663:1749	Our results show the potential of complementary enzyme activities to improve enzymatic hydrolysis of SEB, thus improving the efficiency of the hydrolytic process.
31874680	7	52	theme	commercial	1496:1505	arg1	cellulases					1507:1516	commercial cellulases	1496:1516	commercial cellulases	1496:1516	The supplementation of Accellerase 1500 with hemicellulases enhanced the glucose yield from SEB hydrolysis by 14.6%, but this effect could be raised to 50% when hemicellulases were added prior to hydrolysis with commercial cellulases.
31874680	4	53	from	thermoidea	821:830	arg1	HXYLA					789:793	HXYLA	789:793	HXYLA	789:793	One recombinant endoxylanase (HXYN2) and one recombinant β-xylosidase (HXYLA) from Humicola grisea var thermoidea, together with an α-l-arabinofuranosidase (AFB3) from Penicillium pupurogenum, all produced in Pichia pastoris, were used to formulate an efficient enzyme mixture for SEB hydrolysis using a 23 Central Composite Rotatable Design (CCRD).
31874680	4	53	from	thermoidea	821:830	arg1	β-xylosidase					775:786	one recombinant β-xylosidase	759:786	one recombinant β-xylosidase (HXYLA)	759:794	One recombinant endoxylanase (HXYN2) and one recombinant β-xylosidase (HXYLA) from Humicola grisea var thermoidea, together with an α-l-arabinofuranosidase (AFB3) from Penicillium pupurogenum, all produced in Pichia pastoris, were used to formulate an efficient enzyme mixture for SEB hydrolysis using a 23 Central Composite Rotatable Design (CCRD).
31874680	4	53	from	thermoidea	821:830	arg1	HXYN2					748:752	HXYN2	748:752	HXYN2	748:752	One recombinant endoxylanase (HXYN2) and one recombinant β-xylosidase (HXYLA) from Humicola grisea var thermoidea, together with an α-l-arabinofuranosidase (AFB3) from Penicillium pupurogenum, all produced in Pichia pastoris, were used to formulate an efficient enzyme mixture for SEB hydrolysis using a 23 Central Composite Rotatable Design (CCRD).
31874680	4	53	from	thermoidea	821:830	arg1	endoxylanase					734:745	One recombinant endoxylanase	718:745	One recombinant endoxylanase (HXYN2)	718:753	One recombinant endoxylanase (HXYN2) and one recombinant β-xylosidase (HXYLA) from Humicola grisea var thermoidea, together with an α-l-arabinofuranosidase (AFB3) from Penicillium pupurogenum, all produced in Pichia pastoris, were used to formulate an efficient enzyme mixture for SEB hydrolysis using a 23 Central Composite Rotatable Design (CCRD).
31874680	2	54	theme	energy	353:358	arg1	crops					360:364	energy crops	353:364	energy crops	353:364	Xylan is the main hemicellulose component of energy crops and for its complete hydrolysis a system consisting of several enzymes acting cooperatively, including endoxylanases (XYN), β-xylosidases (XYL) and α-l-arabinofuranosidases (ABF) is necessary.
31874680	4	55	theme	SEB	999:1001	arg1	hydrolysis					1003:1012	SEB hydrolysis	999:1012	SEB hydrolysis using a 23 Central Composite Rotatable Design (CCRD)	999:1065	One recombinant endoxylanase (HXYN2) and one recombinant β-xylosidase (HXYLA) from Humicola grisea var thermoidea, together with an α-l-arabinofuranosidase (AFB3) from Penicillium pupurogenum, all produced in Pichia pastoris, were used to formulate an efficient enzyme mixture for SEB hydrolysis using a 23 Central Composite Rotatable Design (CCRD).
31874680	3	56	theme	steam-exploded	678:691	arg1	SEB					712:714	SEB	712:714	SEB	712:714	The current work aimed at evaluating the effect of recombinant hemicellulolytic enzymes on the enzymatic hydrolysis of steam-exploded sugarcane bagasse (SEB).
31874680	3	56	theme	steam-exploded	678:691	arg1	bagasse					703:709	steam-exploded sugarcane bagasse	678:709	steam-exploded sugarcane bagasse (SEB)	678:715	The current work aimed at evaluating the effect of recombinant hemicellulolytic enzymes on the enzymatic hydrolysis of steam-exploded sugarcane bagasse (SEB).
31874680	6	57	theme	commercial	1190:1199	arg1	cellulases					1201:1210	commercial cellulases	1190:1210	commercial cellulases (Accellerase 1500)	1190:1229	Subsequently, the optimal enzyme mixture was used in combination with commercial cellulases (Accellerase 1500), either simultaneously or in sequential experiments.
31874680	6	57	theme	commercial	1190:1199	arg1	Accellerase					1213:1223	Accellerase	1213:1223	Accellerase	1213:1223	Subsequently, the optimal enzyme mixture was used in combination with commercial cellulases (Accellerase 1500), either simultaneously or in sequential experiments.
31874680	6	58	dep	used	1165:1168	arg1	experiments					1271:1281	sequential experiments	1260:1281	sequential experiments	1260:1281	Subsequently, the optimal enzyme mixture was used in combination with commercial cellulases (Accellerase 1500), either simultaneously or in sequential experiments.
31874680	6	58	dep	used	1165:1168	arg1	either					1232:1237	either	1232:1237	either	1232:1237	Subsequently, the optimal enzyme mixture was used in combination with commercial cellulases (Accellerase 1500), either simultaneously or in sequential experiments.
31874680	6	59	used	used	1165:1168	arg2	mixture					1153:1159	the optimal enzyme mixture	1134:1159	the optimal enzyme mixture	1134:1159	Subsequently, the optimal enzyme mixture was used in combination with commercial cellulases (Accellerase 1500), either simultaneously or in sequential experiments.
31874680	3	60	theme	current	563:569	arg1	work					571:574	The current work	559:574	The current work	559:574	The current work aimed at evaluating the effect of recombinant hemicellulolytic enzymes on the enzymatic hydrolysis of steam-exploded sugarcane bagasse (SEB).
31874680	1	61	from	wall	234:237	arg1	present					208:214	present	208:214	present	208:214	To increase the efficiency of enzyme cocktails in deconstructing cellulose and hemicelluloses present in the plant cell wall, a combination of enzymes with complementary activities is required.
31874680	3	62	theme	sugarcane	693:701	arg1	SEB					712:714	SEB	712:714	SEB	712:714	The current work aimed at evaluating the effect of recombinant hemicellulolytic enzymes on the enzymatic hydrolysis of steam-exploded sugarcane bagasse (SEB).
31874680	3	62	theme	sugarcane	693:701	arg1	bagasse					703:709	steam-exploded sugarcane bagasse	678:709	steam-exploded sugarcane bagasse (SEB)	678:715	The current work aimed at evaluating the effect of recombinant hemicellulolytic enzymes on the enzymatic hydrolysis of steam-exploded sugarcane bagasse (SEB).
31874680	3	63	theme	recombinant	610:620	arg1	enzymes					639:645	recombinant hemicellulolytic enzymes	610:645	recombinant hemicellulolytic enzymes	610:645	The current work aimed at evaluating the effect of recombinant hemicellulolytic enzymes on the enzymatic hydrolysis of steam-exploded sugarcane bagasse (SEB).
31874680	7	64	with	supplementation	1288:1302	arg1	hemicellulases					1329:1342	hemicellulases	1329:1342	hemicellulases	1329:1342	The supplementation of Accellerase 1500 with hemicellulases enhanced the glucose yield from SEB hydrolysis by 14.6%, but this effect could be raised to 50% when hemicellulases were added prior to hydrolysis with commercial cellulases.
31874680	8	65	from	effect	1600:1605	arg1	fibers					1638:1643	SEB fibers	1634:1643	SEB fibers	1634:1643	These results were supported by scanning electron microscopy, which revealed the effect of enzymatic hydrolysis on SEB fibers.
31874680	4	66	from	pupurogenum	898:908	arg1	HXYLA					789:793	HXYLA	789:793	HXYLA	789:793	One recombinant endoxylanase (HXYN2) and one recombinant β-xylosidase (HXYLA) from Humicola grisea var thermoidea, together with an α-l-arabinofuranosidase (AFB3) from Penicillium pupurogenum, all produced in Pichia pastoris, were used to formulate an efficient enzyme mixture for SEB hydrolysis using a 23 Central Composite Rotatable Design (CCRD).
31874680	4	66	from	pupurogenum	898:908	arg1	β-xylosidase					775:786	one recombinant β-xylosidase	759:786	one recombinant β-xylosidase (HXYLA)	759:794	One recombinant endoxylanase (HXYN2) and one recombinant β-xylosidase (HXYLA) from Humicola grisea var thermoidea, together with an α-l-arabinofuranosidase (AFB3) from Penicillium pupurogenum, all produced in Pichia pastoris, were used to formulate an efficient enzyme mixture for SEB hydrolysis using a 23 Central Composite Rotatable Design (CCRD).
31874680	4	66	from	pupurogenum	898:908	arg1	HXYN2					748:752	HXYN2	748:752	HXYN2	748:752	One recombinant endoxylanase (HXYN2) and one recombinant β-xylosidase (HXYLA) from Humicola grisea var thermoidea, together with an α-l-arabinofuranosidase (AFB3) from Penicillium pupurogenum, all produced in Pichia pastoris, were used to formulate an efficient enzyme mixture for SEB hydrolysis using a 23 Central Composite Rotatable Design (CCRD).
31874680	4	66	from	pupurogenum	898:908	arg1	endoxylanase					734:745	One recombinant endoxylanase	718:745	One recombinant endoxylanase (HXYN2)	718:753	One recombinant endoxylanase (HXYN2) and one recombinant β-xylosidase (HXYLA) from Humicola grisea var thermoidea, together with an α-l-arabinofuranosidase (AFB3) from Penicillium pupurogenum, all produced in Pichia pastoris, were used to formulate an efficient enzyme mixture for SEB hydrolysis using a 23 Central Composite Rotatable Design (CCRD).
31874680	3	67	theme	hemicellulolytic	622:637	arg1	enzymes					639:645	recombinant hemicellulolytic enzymes	610:645	recombinant hemicellulolytic enzymes	610:645	The current work aimed at evaluating the effect of recombinant hemicellulolytic enzymes on the enzymatic hydrolysis of steam-exploded sugarcane bagasse (SEB).
31874680	2	68	theme	hemicellulose	326:338	arg1	component					340:348	the main hemicellulose component	317:348	the main hemicellulose component of energy crops	317:364	Xylan is the main hemicellulose component of energy crops and for its complete hydrolysis a system consisting of several enzymes acting cooperatively, including endoxylanases (XYN), β-xylosidases (XYL) and α-l-arabinofuranosidases (ABF) is necessary.
31874680	2	68	theme	hemicellulose	326:338	arg1	Xylan					308:312	Xylan	308:312	Xylan	308:312	Xylan is the main hemicellulose component of energy crops and for its complete hydrolysis a system consisting of several enzymes acting cooperatively, including endoxylanases (XYN), β-xylosidases (XYL) and α-l-arabinofuranosidases (ABF) is necessary.
31874680	4	69	theme	Humicola	801:808	arg1	thermoidea					821:830	Humicola grisea var thermoidea	801:830	Humicola grisea var thermoidea	801:830	One recombinant endoxylanase (HXYN2) and one recombinant β-xylosidase (HXYLA) from Humicola grisea var thermoidea, together with an α-l-arabinofuranosidase (AFB3) from Penicillium pupurogenum, all produced in Pichia pastoris, were used to formulate an efficient enzyme mixture for SEB hydrolysis using a 23 Central Composite Rotatable Design (CCRD).
31874680	1	70	with	combination	242:252	arg1	activities					284:293	complementary activities	270:293	complementary activities	270:293	To increase the efficiency of enzyme cocktails in deconstructing cellulose and hemicelluloses present in the plant cell wall, a combination of enzymes with complementary activities is required.
31874680	1	71	theme	enzyme	144:149	arg1	cocktails					151:159	enzyme cocktails	144:159	enzyme cocktails	144:159	To increase the efficiency of enzyme cocktails in deconstructing cellulose and hemicelluloses present in the plant cell wall, a combination of enzymes with complementary activities is required.
31874680	0	72	theme	enzymatic	57:65	arg1	hydrolysis					67:76	the enzymatic hydrolysis	53:76	the enzymatic hydrolysis of steam-treated sugarcane bagasse	53:111	The boosting effect of recombinant hemicellulases on the enzymatic hydrolysis of steam-treated sugarcane bagasse.
31874680	5	73	theme	SEB	1095:1097	arg1	hydrolysis					1099:1108	SEB hydrolysis	1095:1108	SEB hydrolysis	1095:1108	The most potent enzyme for SEB hydrolysis was ABF3.
31874680	4	74	dep	together	833:840	arg1	with					842:845	with	842:845	with	842:845	One recombinant endoxylanase (HXYN2) and one recombinant β-xylosidase (HXYLA) from Humicola grisea var thermoidea, together with an α-l-arabinofuranosidase (AFB3) from Penicillium pupurogenum, all produced in Pichia pastoris, were used to formulate an efficient enzyme mixture for SEB hydrolysis using a 23 Central Composite Rotatable Design (CCRD).
31874680	1	75	theme	cocktails	151:159	arg1	efficiency					130:139	the efficiency	126:139	the efficiency of enzyme cocktails	126:159	To increase the efficiency of enzyme cocktails in deconstructing cellulose and hemicelluloses present in the plant cell wall, a combination of enzymes with complementary activities is required.
31874680	4	76	theme	Rotatable	1043:1051	arg1	Design					1053:1058	a 23 Central Composite Rotatable Design	1020:1058	a 23 Central Composite Rotatable Design (CCRD)	1020:1065	One recombinant endoxylanase (HXYN2) and one recombinant β-xylosidase (HXYLA) from Humicola grisea var thermoidea, together with an α-l-arabinofuranosidase (AFB3) from Penicillium pupurogenum, all produced in Pichia pastoris, were used to formulate an efficient enzyme mixture for SEB hydrolysis using a 23 Central Composite Rotatable Design (CCRD).
31874680	4	76	theme	Rotatable	1043:1051	arg1	CCRD					1061:1064	CCRD	1061:1064	CCRD	1061:1064	One recombinant endoxylanase (HXYN2) and one recombinant β-xylosidase (HXYLA) from Humicola grisea var thermoidea, together with an α-l-arabinofuranosidase (AFB3) from Penicillium pupurogenum, all produced in Pichia pastoris, were used to formulate an efficient enzyme mixture for SEB hydrolysis using a 23 Central Composite Rotatable Design (CCRD).
32255048	2	0	from	emergencies	380:390	arg1	present					369:375	present	369:375	present	369:375	Chitosan (CS) has been widely used as a popular hemostatic dressing; however, irregularly shaped wounds present in emergencies limit the performance of CS powder.
32255048	11	1	with	performance	1768:1778	arg1	tissues					1869:1875	decreased granulation tissues	1847:1875	decreased granulation tissues	1847:1875	Furthermore, in vivo evaluation of the PVA-CS sponges revealed excellent hemostatic performance and enhanced wound healing with increased re-epithelialization and decreased granulation tissues.
32255048	11	1	with	performance	1768:1778	arg1	re-epithelialization					1822:1841	increased re-epithelialization	1812:1841	increased re-epithelialization	1812:1841	Furthermore, in vivo evaluation of the PVA-CS sponges revealed excellent hemostatic performance and enhanced wound healing with increased re-epithelialization and decreased granulation tissues.
32255048	2	2	theme	hemostatic	313:322	arg1	dressing					324:331	a popular hemostatic dressing	303:331	a popular hemostatic dressing	303:331	Chitosan (CS) has been widely used as a popular hemostatic dressing; however, irregularly shaped wounds present in emergencies limit the performance of CS powder.
32255048	2	2	theme	hemostatic	313:322	arg1	Chitosan					265:272	Chitosan	265:272	Chitosan (CS)	265:277	Chitosan (CS) has been widely used as a popular hemostatic dressing; however, irregularly shaped wounds present in emergencies limit the performance of CS powder.
32255048	9	3	theme	platelet	1528:1535	arg1	adhesion					1537:1544	blood cell and platelet adhesion	1513:1544	adhesion	1537:1544	As compared with gauze and PVA, which are widely used in first aid, PVA-CS sponges showed an improved blood clotting ability and increased blood cell and platelet adhesion and activation.
32255048	10	4	theme	cell	1634:1637	arg1	proliferation					1639:1651	cell proliferation	1634:1651	cell proliferation	1634:1651	The PVA-CS sponges also showed high biocompatibility in cell viability, cell proliferation and cell attachment bioassays.
32255048	6	5	theme	dermal	1072:1077	arg1	testing					1101:1107	human dermal fibroblast (HDF) cell testing	1066:1107	human dermal fibroblast (HDF) cell testing	1066:1107	In vitro, the swelling ratio and blood clotting ability were evaluated in different groups with various weight ratios or degrees of deacetylation of the CS, and the cytocompatibility and cell attachment on the material were analyzed by human dermal fibroblast (HDF) cell testing.
32255048	10	6	theme	viability	1623:1631	arg1	bioassays					1673:1681	cell viability, cell proliferation and cell attachment bioassays	1618:1681	cell viability, cell proliferation and cell attachment bioassays	1618:1681	The PVA-CS sponges also showed high biocompatibility in cell viability, cell proliferation and cell attachment bioassays.
32255048	1	7	theme	effective	158:166	arg1	hemostasis					168:177	Rapid and effective hemostasis	148:177	Rapid and effective hemostasis for a noncompressible hemorrhage	148:210	Rapid and effective hemostasis for a noncompressible hemorrhage is the key to control bleeding and reduce mortality.
32255048	1	7	theme	effective	158:166	arg1	key					219:221	key	219:221	key	219:221	Rapid and effective hemostasis for a noncompressible hemorrhage is the key to control bleeding and reduce mortality.
32255048	11	8	with	healing	1799:1805	arg1	tissues					1869:1875	decreased granulation tissues	1847:1875	decreased granulation tissues	1847:1875	Furthermore, in vivo evaluation of the PVA-CS sponges revealed excellent hemostatic performance and enhanced wound healing with increased re-epithelialization and decreased granulation tissues.
32255048	11	8	with	healing	1799:1805	arg1	re-epithelialization					1822:1841	increased re-epithelialization	1812:1841	increased re-epithelialization	1812:1841	Furthermore, in vivo evaluation of the PVA-CS sponges revealed excellent hemostatic performance and enhanced wound healing with increased re-epithelialization and decreased granulation tissues.
32255048	10	9	theme	attachment	1662:1671	arg1	bioassays					1673:1681	cell viability, cell proliferation and cell attachment bioassays	1618:1681	cell viability, cell proliferation and cell attachment bioassays	1618:1681	The PVA-CS sponges also showed high biocompatibility in cell viability, cell proliferation and cell attachment bioassays.
32255048	9	10	used	used	1423:1426	arg2	PVA					1401:1403	PVA	1401:1403	PVA	1401:1403	As compared with gauze and PVA, which are widely used in first aid, PVA-CS sponges showed an improved blood clotting ability and increased blood cell and platelet adhesion and activation.
32255048	9	10	used	used	1423:1426	arg2	gauze					1391:1395	gauze	1391:1395	gauze	1391:1395	As compared with gauze and PVA, which are widely used in first aid, PVA-CS sponges showed an improved blood clotting ability and increased blood cell and platelet adhesion and activation.
32255048	10	11	theme	high	1593:1596	arg1	biocompatibility					1598:1613	high biocompatibility	1593:1613	high biocompatibility	1593:1613	The PVA-CS sponges also showed high biocompatibility in cell viability, cell proliferation and cell attachment bioassays.
32255048	1	12	theme	noncompressible	185:199	arg1	hemorrhage					201:210	a noncompressible hemorrhage	183:210	a noncompressible hemorrhage	183:210	Rapid and effective hemostasis for a noncompressible hemorrhage is the key to control bleeding and reduce mortality.
32255048	6	13	theme	blood	863:867	arg1	ability					878:884	blood clotting ability	863:884	blood clotting ability	863:884	In vitro, the swelling ratio and blood clotting ability were evaluated in different groups with various weight ratios or degrees of deacetylation of the CS, and the cytocompatibility and cell attachment on the material were analyzed by human dermal fibroblast (HDF) cell testing.
32255048	4	14	theme	novel	562:566	arg1	PVA-CS					578:583	The novel synthetic PVA-CS	558:583	The novel synthetic PVA-CS	558:583	The novel synthetic PVA-CS was prepared by cross-linking PVA and CS during foaming and crosslinking reactions.
32255048	8	15	theme	fast	1351:1354	arg1	speed					1367:1371	a fast absorption speed	1349:1371	a fast absorption speed	1349:1371	PVA-CS presents robust mechanical strength, rapid water-triggered swelling and a fast absorption speed.
32255048	7	16	theme	wound	1252:1256	arg1	experiment					1258:1267	gunshot wound experiment	1244:1267	gunshot wound experiment	1244:1267	In vivo, the hemostatic effects were evaluated in Sprague-Dawley rats and Bama miniature pigs in a femoral artery hemorrhage model or gunshot wound experiment.
32255048	9	17	dep	first	1431:1435	arg1	aid					1437:1439	aid	1437:1439	aid	1437:1439	As compared with gauze and PVA, which are widely used in first aid, PVA-CS sponges showed an improved blood clotting ability and increased blood cell and platelet adhesion and activation.
32255048	0	18	theme	high	125:128	arg1	biocompatibility					130:145	high biocompatibility	125:145	high biocompatibility	125:145	Synthetic poly(vinyl alcohol)-chitosan as a new type of highly efficient hemostatic sponge with blood-triggered swelling and high biocompatibility.
32255048	6	19	theme	weight	934:939	arg1	ratios					941:946	various weight ratios	926:946	various weight ratios	926:946	In vitro, the swelling ratio and blood clotting ability were evaluated in different groups with various weight ratios or degrees of deacetylation of the CS, and the cytocompatibility and cell attachment on the material were analyzed by human dermal fibroblast (HDF) cell testing.
32255048	6	20	with	groups	914:919	arg1	degrees					951:957	degrees	951:957	degrees of deacetylation of the CS	951:984	In vitro, the swelling ratio and blood clotting ability were evaluated in different groups with various weight ratios or degrees of deacetylation of the CS, and the cytocompatibility and cell attachment on the material were analyzed by human dermal fibroblast (HDF) cell testing.
32255048	6	20	with	groups	914:919	arg1	ratios					941:946	various weight ratios	926:946	various weight ratios	926:946	In vitro, the swelling ratio and blood clotting ability were evaluated in different groups with various weight ratios or degrees of deacetylation of the CS, and the cytocompatibility and cell attachment on the material were analyzed by human dermal fibroblast (HDF) cell testing.
32255048	6	21	theme	deacetylation	962:974	arg1	degrees					951:957	degrees	951:957	degrees of deacetylation of the CS	951:984	In vitro, the swelling ratio and blood clotting ability were evaluated in different groups with various weight ratios or degrees of deacetylation of the CS, and the cytocompatibility and cell attachment on the material were analyzed by human dermal fibroblast (HDF) cell testing.
32255048	6	21	theme	deacetylation	962:974	arg1	ratios					941:946	various weight ratios	926:946	various weight ratios	926:946	In vitro, the swelling ratio and blood clotting ability were evaluated in different groups with various weight ratios or degrees of deacetylation of the CS, and the cytocompatibility and cell attachment on the material were analyzed by human dermal fibroblast (HDF) cell testing.
32255048	5	22	theme	infrared	687:694	arg1	FTIR					710:713	FTIR	710:713	FTIR	710:713	Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and X-ray diffraction were utilized to analyze the characteristics of PVA-CS.
32255048	5	22	theme	infrared	687:694	arg1	spectroscopy					696:707	infrared spectroscopy	687:707	infrared spectroscopy (FTIR)	687:714	Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and X-ray diffraction were utilized to analyze the characteristics of PVA-CS.
32255048	12	23	from	hemorrhage	1976:1985	arg1	trauma					1996:2001	acute trauma	1990:2001	acute trauma	1990:2001	The results of this study strongly support the use of these composite sponges for noncompressible hemorrhage in acute trauma and ballistic injuries.
32255048	12	23	from	hemorrhage	1976:1985	arg1	injuries					2017:2024	ballistic injuries	2007:2024	ballistic injuries	2007:2024	The results of this study strongly support the use of these composite sponges for noncompressible hemorrhage in acute trauma and ballistic injuries.
32255048	12	24	theme	acute	1990:1994	arg1	trauma					1996:2001	acute trauma	1990:2001	acute trauma	1990:2001	The results of this study strongly support the use of these composite sponges for noncompressible hemorrhage in acute trauma and ballistic injuries.
32255048	1	25	theme	Rapid	148:152	arg1	hemostasis					168:177	Rapid and effective hemostasis	148:177	Rapid and effective hemostasis for a noncompressible hemorrhage	148:210	Rapid and effective hemostasis for a noncompressible hemorrhage is the key to control bleeding and reduce mortality.
32255048	1	25	theme	Rapid	148:152	arg1	key					219:221	key	219:221	key	219:221	Rapid and effective hemostasis for a noncompressible hemorrhage is the key to control bleeding and reduce mortality.
32255048	6	26	theme	different	904:912	arg1	groups					914:919	different groups	904:919	different groups with various weight ratios or degrees of deacetylation of the CS	904:984	In vitro, the swelling ratio and blood clotting ability were evaluated in different groups with various weight ratios or degrees of deacetylation of the CS, and the cytocompatibility and cell attachment on the material were analyzed by human dermal fibroblast (HDF) cell testing.
32255048	6	27	theme	cell	1096:1099	arg1	testing					1101:1107	human dermal fibroblast (HDF) cell testing	1066:1107	human dermal fibroblast (HDF) cell testing	1066:1107	In vitro, the swelling ratio and blood clotting ability were evaluated in different groups with various weight ratios or degrees of deacetylation of the CS, and the cytocompatibility and cell attachment on the material were analyzed by human dermal fibroblast (HDF) cell testing.
32255048	0	28	theme	hemostatic	73:82	arg1	sponge					84:89	highly efficient hemostatic sponge	56:89	highly efficient hemostatic sponge	56:89	Synthetic poly(vinyl alcohol)-chitosan as a new type of highly efficient hemostatic sponge with blood-triggered swelling and high biocompatibility.
32255048	6	29	theme	CS	983:984	arg1	deacetylation					962:974	deacetylation	962:974	deacetylation of the CS	962:984	In vitro, the swelling ratio and blood clotting ability were evaluated in different groups with various weight ratios or degrees of deacetylation of the CS, and the cytocompatibility and cell attachment on the material were analyzed by human dermal fibroblast (HDF) cell testing.
32255048	2	30	theme	CS	417:418	arg1	powder					420:425	CS powder	417:425	CS powder	417:425	Chitosan (CS) has been widely used as a popular hemostatic dressing; however, irregularly shaped wounds present in emergencies limit the performance of CS powder.
32255048	11	31	theme	wound	1793:1797	arg1	healing					1799:1805	enhanced wound healing	1784:1805	enhanced wound healing	1784:1805	Furthermore, in vivo evaluation of the PVA-CS sponges revealed excellent hemostatic performance and enhanced wound healing with increased re-epithelialization and decreased granulation tissues.
32255048	0	32	theme	Synthetic	0:8	arg1	poly					10:13	Synthetic poly	0:13	Synthetic poly(vinyl alcohol)	0:28	Synthetic poly(vinyl alcohol)-chitosan as a new type of highly efficient hemostatic sponge with blood-triggered swelling and high biocompatibility.
32255048	0	32	theme	Synthetic	0:8	arg1	alcohol					21:27	vinyl alcohol	15:27	vinyl alcohol	15:27	Synthetic poly(vinyl alcohol)-chitosan as a new type of highly efficient hemostatic sponge with blood-triggered swelling and high biocompatibility.
32255048	9	33	theme	PVA-CS	1442:1447	arg1	sponges					1449:1455	PVA-CS sponges	1442:1455	PVA-CS sponges	1442:1455	As compared with gauze and PVA, which are widely used in first aid, PVA-CS sponges showed an improved blood clotting ability and increased blood cell and platelet adhesion and activation.
32255048	7	34	theme	hemorrhage	1224:1233	arg1	model					1235:1239	a femoral artery hemorrhage model	1207:1239	a femoral artery hemorrhage model	1207:1239	In vivo, the hemostatic effects were evaluated in Sprague-Dawley rats and Bama miniature pigs in a femoral artery hemorrhage model or gunshot wound experiment.
32255048	11	35	theme	decreased	1847:1855	arg1	tissues					1869:1875	decreased granulation tissues	1847:1875	decreased granulation tissues	1847:1875	Furthermore, in vivo evaluation of the PVA-CS sponges revealed excellent hemostatic performance and enhanced wound healing with increased re-epithelialization and decreased granulation tissues.
32255048	8	36	theme	robust	1286:1291	arg1	strength					1304:1311	robust mechanical strength	1286:1311	robust mechanical strength	1286:1311	PVA-CS presents robust mechanical strength, rapid water-triggered swelling and a fast absorption speed.
32255048	2	37	attach	present	369:375	arg1	emergencies					380:390	emergencies	380:390	emergencies	380:390	Chitosan (CS) has been widely used as a popular hemostatic dressing; however, irregularly shaped wounds present in emergencies limit the performance of CS powder.
32255048	2	37	attach	present	369:375	arg2	wounds					362:367	irregularly shaped wounds	343:367	irregularly shaped wounds present in emergencies	343:390	Chitosan (CS) has been widely used as a popular hemostatic dressing; however, irregularly shaped wounds present in emergencies limit the performance of CS powder.
32255048	9	38	theme	improved	1467:1474	arg1	ability					1491:1497	an improved blood clotting ability	1464:1497	an improved blood clotting ability	1464:1497	As compared with gauze and PVA, which are widely used in first aid, PVA-CS sponges showed an improved blood clotting ability and increased blood cell and platelet adhesion and activation.
32255048	7	39	theme	Bama	1184:1187	arg1	pigs					1199:1202	Bama miniature pigs	1184:1202	Bama miniature pigs	1184:1202	In vivo, the hemostatic effects were evaluated in Sprague-Dawley rats and Bama miniature pigs in a femoral artery hemorrhage model or gunshot wound experiment.
32255048	2	40	theme	popular	305:311	arg1	dressing					324:331	a popular hemostatic dressing	303:331	a popular hemostatic dressing	303:331	Chitosan (CS) has been widely used as a popular hemostatic dressing; however, irregularly shaped wounds present in emergencies limit the performance of CS powder.
32255048	2	40	theme	popular	305:311	arg1	Chitosan					265:272	Chitosan	265:272	Chitosan (CS)	265:277	Chitosan (CS) has been widely used as a popular hemostatic dressing; however, irregularly shaped wounds present in emergencies limit the performance of CS powder.
32255048	5	41	theme	scanning	717:724	arg1	microscopy					735:744	scanning electron microscopy	717:744	scanning electron microscopy (SEM)	717:750	Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and X-ray diffraction were utilized to analyze the characteristics of PVA-CS.
32255048	5	41	theme	scanning	717:724	arg1	SEM					747:749	SEM	747:749	SEM	747:749	Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and X-ray diffraction were utilized to analyze the characteristics of PVA-CS.
32255048	9	42	theme	clotting	1482:1489	arg1	ability					1491:1497	an improved blood clotting ability	1464:1497	an improved blood clotting ability	1464:1497	As compared with gauze and PVA, which are widely used in first aid, PVA-CS sponges showed an improved blood clotting ability and increased blood cell and platelet adhesion and activation.
32255048	8	43	theme	rapid	1314:1318	arg1	swelling					1336:1343	rapid water-triggered swelling	1314:1343	rapid water-triggered swelling	1314:1343	PVA-CS presents robust mechanical strength, rapid water-triggered swelling and a fast absorption speed.
32255048	11	44	theme	PVA-CS	1723:1728	arg1	sponges					1730:1736	the PVA-CS sponges	1719:1736	the PVA-CS sponges	1719:1736	Furthermore, in vivo evaluation of the PVA-CS sponges revealed excellent hemostatic performance and enhanced wound healing with increased re-epithelialization and decreased granulation tissues.
32255048	6	45	theme	cell	1017:1020	arg1	attachment					1022:1031	cell attachment	1017:1031	cell attachment	1017:1031	In vitro, the swelling ratio and blood clotting ability were evaluated in different groups with various weight ratios or degrees of deacetylation of the CS, and the cytocompatibility and cell attachment on the material were analyzed by human dermal fibroblast (HDF) cell testing.
32255048	11	46	theme	hemostatic	1757:1766	arg1	performance					1768:1778	excellent hemostatic performance	1747:1778	excellent hemostatic performance	1747:1778	Furthermore, in vivo evaluation of the PVA-CS sponges revealed excellent hemostatic performance and enhanced wound healing with increased re-epithelialization and decreased granulation tissues.
32255048	10	47	theme	cell	1618:1621	arg1	viability					1623:1631	cell viability	1618:1631	cell viability	1618:1631	The PVA-CS sponges also showed high biocompatibility in cell viability, cell proliferation and cell attachment bioassays.
32255048	9	48	theme	blood	1513:1517	arg1	cell					1519:1522	blood cell and platelet adhesion	1513:1544	cell	1519:1522	As compared with gauze and PVA, which are widely used in first aid, PVA-CS sponges showed an improved blood clotting ability and increased blood cell and platelet adhesion and activation.
32255048	6	49	from	cytocompatibility	995:1011	arg1	material					1040:1047	the material	1036:1047	the material	1036:1047	In vitro, the swelling ratio and blood clotting ability were evaluated in different groups with various weight ratios or degrees of deacetylation of the CS, and the cytocompatibility and cell attachment on the material were analyzed by human dermal fibroblast (HDF) cell testing.
32255048	3	50	theme	CS	464:465	arg1	effect					454:459	the hemostatic effect	439:459	the hemostatic effect of CS	439:465	To improve the hemostatic effect of CS, we modified it with poly(vinyl alcohol) (PVA), a fast-swelling sponge triggered by water.
32255048	12	51	theme	sponges	1948:1954	arg1	use					1925:1927	the use	1921:1927	the use of these composite sponges for noncompressible hemorrhage in acute trauma and ballistic injuries	1921:2024	The results of this study strongly support the use of these composite sponges for noncompressible hemorrhage in acute trauma and ballistic injuries.
32255048	7	52	theme	hemostatic	1123:1132	arg1	effects					1134:1140	the hemostatic effects	1119:1140	the hemostatic effects	1119:1140	In vivo, the hemostatic effects were evaluated in Sprague-Dawley rats and Bama miniature pigs in a femoral artery hemorrhage model or gunshot wound experiment.
32255048	11	53	theme	increased	1812:1820	arg1	re-epithelialization					1822:1841	increased re-epithelialization	1812:1841	increased re-epithelialization	1812:1841	Furthermore, in vivo evaluation of the PVA-CS sponges revealed excellent hemostatic performance and enhanced wound healing with increased re-epithelialization and decreased granulation tissues.
32255048	10	54	theme	proliferation	1639:1651	arg1	bioassays					1673:1681	cell viability, cell proliferation and cell attachment bioassays	1618:1681	cell viability, cell proliferation and cell attachment bioassays	1618:1681	The PVA-CS sponges also showed high biocompatibility in cell viability, cell proliferation and cell attachment bioassays.
32255048	11	55	theme	sponges	1730:1736	arg1	evaluation					1705:1714	in vivo evaluation	1697:1714	in vivo evaluation of the PVA-CS sponges	1697:1736	Furthermore, in vivo evaluation of the PVA-CS sponges revealed excellent hemostatic performance and enhanced wound healing with increased re-epithelialization and decreased granulation tissues.
32255048	6	56	theme	fibroblast	1079:1088	arg1	testing					1101:1107	human dermal fibroblast (HDF) cell testing	1066:1107	human dermal fibroblast (HDF) cell testing	1066:1107	In vitro, the swelling ratio and blood clotting ability were evaluated in different groups with various weight ratios or degrees of deacetylation of the CS, and the cytocompatibility and cell attachment on the material were analyzed by human dermal fibroblast (HDF) cell testing.
32255048	6	57	theme	human	1066:1070	arg1	testing					1101:1107	human dermal fibroblast (HDF) cell testing	1066:1107	human dermal fibroblast (HDF) cell testing	1066:1107	In vitro, the swelling ratio and blood clotting ability were evaluated in different groups with various weight ratios or degrees of deacetylation of the CS, and the cytocompatibility and cell attachment on the material were analyzed by human dermal fibroblast (HDF) cell testing.
32255048	4	58	theme	synthetic	568:576	arg1	PVA-CS					578:583	The novel synthetic PVA-CS	558:583	The novel synthetic PVA-CS	558:583	The novel synthetic PVA-CS was prepared by cross-linking PVA and CS during foaming and crosslinking reactions.
32255048	11	59	theme	in	1697:1698	arg1	evaluation					1705:1714	in vivo evaluation	1697:1714	in vivo evaluation of the PVA-CS sponges	1697:1736	Furthermore, in vivo evaluation of the PVA-CS sponges revealed excellent hemostatic performance and enhanced wound healing with increased re-epithelialization and decreased granulation tissues.
32255048	0	60	theme	blood-triggered	96:110	arg1	swelling					112:119	blood-triggered swelling	96:119	blood-triggered swelling	96:119	Synthetic poly(vinyl alcohol)-chitosan as a new type of highly efficient hemostatic sponge with blood-triggered swelling and high biocompatibility.
32255048	6	61	dep	cytocompatibility	995:1011	arg1	the					991:993	the	991:993	the	991:993	In vitro, the swelling ratio and blood clotting ability were evaluated in different groups with various weight ratios or degrees of deacetylation of the CS, and the cytocompatibility and cell attachment on the material were analyzed by human dermal fibroblast (HDF) cell testing.
32255048	3	62	theme	vinyl	493:497	arg1	poly					488:491	poly	488:491	poly(vinyl alcohol) (PVA)	488:512	To improve the hemostatic effect of CS, we modified it with poly(vinyl alcohol) (PVA), a fast-swelling sponge triggered by water.
32255048	3	62	theme	vinyl	493:497	arg1	alcohol					499:505	vinyl alcohol	493:505	vinyl alcohol	493:505	To improve the hemostatic effect of CS, we modified it with poly(vinyl alcohol) (PVA), a fast-swelling sponge triggered by water.
32255048	12	63	theme	study	1898:1902	arg1	results					1882:1888	The results	1878:1888	The results of this study	1878:1902	The results of this study strongly support the use of these composite sponges for noncompressible hemorrhage in acute trauma and ballistic injuries.
32255048	6	64	theme	clotting	869:876	arg1	ability					878:884	blood clotting ability	863:884	blood clotting ability	863:884	In vitro, the swelling ratio and blood clotting ability were evaluated in different groups with various weight ratios or degrees of deacetylation of the CS, and the cytocompatibility and cell attachment on the material were analyzed by human dermal fibroblast (HDF) cell testing.
32255048	5	65	theme	PVA-CS	822:827	arg1	characteristics					803:817	the characteristics	799:817	the characteristics of PVA-CS	799:827	Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and X-ray diffraction were utilized to analyze the characteristics of PVA-CS.
32255048	5	66	theme	X-ray	756:760	arg1	diffraction					762:772	X-ray diffraction	756:772	X-ray diffraction	756:772	Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and X-ray diffraction were utilized to analyze the characteristics of PVA-CS.
32255048	8	67	theme	absorption	1356:1365	arg1	speed					1367:1371	a fast absorption speed	1349:1371	a fast absorption speed	1349:1371	PVA-CS presents robust mechanical strength, rapid water-triggered swelling and a fast absorption speed.
32255048	6	68	theme	swelling	844:851	arg1	ratio					853:857	the swelling ratio	840:857	the swelling ratio	840:857	In vitro, the swelling ratio and blood clotting ability were evaluated in different groups with various weight ratios or degrees of deacetylation of the CS, and the cytocompatibility and cell attachment on the material were analyzed by human dermal fibroblast (HDF) cell testing.
32255048	5	69	dep	Fourier	669:675	arg1	transform					677:685	transform	677:685	transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and X-ray diffraction	677:772	Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and X-ray diffraction were utilized to analyze the characteristics of PVA-CS.
32255048	10	70	theme	cell	1657:1660	arg1	attachment					1662:1671	cell attachment	1657:1671	cell attachment	1657:1671	The PVA-CS sponges also showed high biocompatibility in cell viability, cell proliferation and cell attachment bioassays.
32255048	7	71	theme	gunshot	1244:1250	arg1	experiment					1258:1267	gunshot wound experiment	1244:1267	gunshot wound experiment	1244:1267	In vivo, the hemostatic effects were evaluated in Sprague-Dawley rats and Bama miniature pigs in a femoral artery hemorrhage model or gunshot wound experiment.
32255048	12	72	theme	ballistic	2007:2015	arg1	injuries					2017:2024	ballistic injuries	2007:2024	ballistic injuries	2007:2024	The results of this study strongly support the use of these composite sponges for noncompressible hemorrhage in acute trauma and ballistic injuries.
32255048	0	73	theme	new	44:46	arg1	type					48:51	a new type	42:51	a new type of highly efficient hemostatic sponge	42:89	Synthetic poly(vinyl alcohol)-chitosan as a new type of highly efficient hemostatic sponge with blood-triggered swelling and high biocompatibility.
32255048	3	74	theme	fast-swelling	517:529	arg1	poly					488:491	poly	488:491	poly(vinyl alcohol) (PVA)	488:512	To improve the hemostatic effect of CS, we modified it with poly(vinyl alcohol) (PVA), a fast-swelling sponge triggered by water.
32255048	3	74	theme	fast-swelling	517:529	arg1	sponge					531:536	a fast-swelling sponge	515:536	a fast-swelling sponge triggered by water	515:555	To improve the hemostatic effect of CS, we modified it with poly(vinyl alcohol) (PVA), a fast-swelling sponge triggered by water.
32255048	6	75	theme	various	926:932	arg1	ratios					941:946	various weight ratios	926:946	various weight ratios	926:946	In vitro, the swelling ratio and blood clotting ability were evaluated in different groups with various weight ratios or degrees of deacetylation of the CS, and the cytocompatibility and cell attachment on the material were analyzed by human dermal fibroblast (HDF) cell testing.
32255048	6	76	theme	HDF	1091:1093	arg1	testing					1101:1107	human dermal fibroblast (HDF) cell testing	1066:1107	human dermal fibroblast (HDF) cell testing	1066:1107	In vitro, the swelling ratio and blood clotting ability were evaluated in different groups with various weight ratios or degrees of deacetylation of the CS, and the cytocompatibility and cell attachment on the material were analyzed by human dermal fibroblast (HDF) cell testing.
32255048	12	77	theme	noncompressible	1960:1974	arg1	hemorrhage					1976:1985	noncompressible hemorrhage	1960:1985	noncompressible hemorrhage in acute trauma and ballistic injuries	1960:2024	The results of this study strongly support the use of these composite sponges for noncompressible hemorrhage in acute trauma and ballistic injuries.
32255048	5	78	used	utilized	779:786	arg2	Fourier					669:675	Fourier	669:675	Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and X-ray diffraction	669:772	Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and X-ray diffraction were utilized to analyze the characteristics of PVA-CS.
32255048	0	79	theme	efficient	63:71	arg1	sponge					84:89	highly efficient hemostatic sponge	56:89	highly efficient hemostatic sponge	56:89	Synthetic poly(vinyl alcohol)-chitosan as a new type of highly efficient hemostatic sponge with blood-triggered swelling and high biocompatibility.
32255048	11	80	dep	in	1697:1698	arg1	vivo					1700:1703	vivo	1700:1703	vivo	1700:1703	Furthermore, in vivo evaluation of the PVA-CS sponges revealed excellent hemostatic performance and enhanced wound healing with increased re-epithelialization and decreased granulation tissues.
32255048	3	81	theme	hemostatic	443:452	arg1	effect					454:459	the hemostatic effect	439:459	the hemostatic effect of CS	439:465	To improve the hemostatic effect of CS, we modified it with poly(vinyl alcohol) (PVA), a fast-swelling sponge triggered by water.
32255048	0	82	theme	sponge	84:89	arg1	type					48:51	a new type	42:51	a new type of highly efficient hemostatic sponge	42:89	Synthetic poly(vinyl alcohol)-chitosan as a new type of highly efficient hemostatic sponge with blood-triggered swelling and high biocompatibility.
32255048	7	83	theme	femoral	1209:1215	arg1	model					1235:1239	a femoral artery hemorrhage model	1207:1239	a femoral artery hemorrhage model	1207:1239	In vivo, the hemostatic effects were evaluated in Sprague-Dawley rats and Bama miniature pigs in a femoral artery hemorrhage model or gunshot wound experiment.
32255048	2	84	used	used	295:298	arg2	dressing					324:331	a popular hemostatic dressing	303:331	a popular hemostatic dressing	303:331	Chitosan (CS) has been widely used as a popular hemostatic dressing; however, irregularly shaped wounds present in emergencies limit the performance of CS powder.
32255048	2	84	used	used	295:298	arg2	CS					275:276	CS	275:276	CS	275:276	Chitosan (CS) has been widely used as a popular hemostatic dressing; however, irregularly shaped wounds present in emergencies limit the performance of CS powder.
32255048	2	84	used	used	295:298	arg2	Chitosan					265:272	Chitosan	265:272	Chitosan (CS)	265:277	Chitosan (CS) has been widely used as a popular hemostatic dressing; however, irregularly shaped wounds present in emergencies limit the performance of CS powder.
32255048	2	85	theme	shaped	355:360	arg1	wounds					362:367	irregularly shaped wounds	343:367	irregularly shaped wounds present in emergencies	343:390	Chitosan (CS) has been widely used as a popular hemostatic dressing; however, irregularly shaped wounds present in emergencies limit the performance of CS powder.
32255048	5	86	theme	electron	726:733	arg1	microscopy					735:744	scanning electron microscopy	717:744	scanning electron microscopy (SEM)	717:750	Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and X-ray diffraction were utilized to analyze the characteristics of PVA-CS.
32255048	5	86	theme	electron	726:733	arg1	SEM					747:749	SEM	747:749	SEM	747:749	Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and X-ray diffraction were utilized to analyze the characteristics of PVA-CS.
32255048	4	87	theme	crosslinking	645:656	arg1	reactions					658:666	crosslinking reactions	645:666	crosslinking reactions	645:666	The novel synthetic PVA-CS was prepared by cross-linking PVA and CS during foaming and crosslinking reactions.
32255048	0	88	theme	vinyl	15:19	arg1	poly					10:13	Synthetic poly	0:13	Synthetic poly(vinyl alcohol)	0:28	Synthetic poly(vinyl alcohol)-chitosan as a new type of highly efficient hemostatic sponge with blood-triggered swelling and high biocompatibility.
32255048	0	88	theme	vinyl	15:19	arg1	alcohol					21:27	vinyl alcohol	15:27	vinyl alcohol	15:27	Synthetic poly(vinyl alcohol)-chitosan as a new type of highly efficient hemostatic sponge with blood-triggered swelling and high biocompatibility.
32255048	7	89	theme	miniature	1189:1197	arg1	pigs					1199:1202	Bama miniature pigs	1184:1202	Bama miniature pigs	1184:1202	In vivo, the hemostatic effects were evaluated in Sprague-Dawley rats and Bama miniature pigs in a femoral artery hemorrhage model or gunshot wound experiment.
32255048	11	90	theme	granulation	1857:1867	arg1	tissues					1869:1875	decreased granulation tissues	1847:1875	decreased granulation tissues	1847:1875	Furthermore, in vivo evaluation of the PVA-CS sponges revealed excellent hemostatic performance and enhanced wound healing with increased re-epithelialization and decreased granulation tissues.
32255048	6	91	from	attachment	1022:1031	arg1	material					1040:1047	the material	1036:1047	the material	1036:1047	In vitro, the swelling ratio and blood clotting ability were evaluated in different groups with various weight ratios or degrees of deacetylation of the CS, and the cytocompatibility and cell attachment on the material were analyzed by human dermal fibroblast (HDF) cell testing.
32255048	2	92	theme	present	369:375	arg1	wounds					362:367	irregularly shaped wounds	343:367	irregularly shaped wounds present in emergencies	343:390	Chitosan (CS) has been widely used as a popular hemostatic dressing; however, irregularly shaped wounds present in emergencies limit the performance of CS powder.
32255048	9	93	theme	blood	1476:1480	arg1	ability					1491:1497	an improved blood clotting ability	1464:1497	an improved blood clotting ability	1464:1497	As compared with gauze and PVA, which are widely used in first aid, PVA-CS sponges showed an improved blood clotting ability and increased blood cell and platelet adhesion and activation.
32255048	7	94	theme	Sprague-Dawley	1160:1173	arg1	rats					1175:1178	Sprague-Dawley rats	1160:1178	Sprague-Dawley rats	1160:1178	In vivo, the hemostatic effects were evaluated in Sprague-Dawley rats and Bama miniature pigs in a femoral artery hemorrhage model or gunshot wound experiment.
32255048	2	95	theme	powder	420:425	arg1	performance					402:412	the performance	398:412	the performance of CS powder	398:425	Chitosan (CS) has been widely used as a popular hemostatic dressing; however, irregularly shaped wounds present in emergencies limit the performance of CS powder.
32255048	8	96	theme	mechanical	1293:1302	arg1	strength					1304:1311	robust mechanical strength	1286:1311	robust mechanical strength	1286:1311	PVA-CS presents robust mechanical strength, rapid water-triggered swelling and a fast absorption speed.
32255048	11	97	theme	excellent	1747:1755	arg1	performance					1768:1778	excellent hemostatic performance	1747:1778	excellent hemostatic performance	1747:1778	Furthermore, in vivo evaluation of the PVA-CS sponges revealed excellent hemostatic performance and enhanced wound healing with increased re-epithelialization and decreased granulation tissues.
32255048	10	98	theme	PVA-CS	1566:1571	arg1	sponges					1573:1579	The PVA-CS sponges	1562:1579	The PVA-CS sponges	1562:1579	The PVA-CS sponges also showed high biocompatibility in cell viability, cell proliferation and cell attachment bioassays.
32255048	12	99	theme	composite	1938:1946	arg1	sponges					1948:1954	these composite sponges	1932:1954	these composite sponges	1932:1954	The results of this study strongly support the use of these composite sponges for noncompressible hemorrhage in acute trauma and ballistic injuries.
32255048	2	100	from	present	369:375	arg1	emergencies					380:390	emergencies	380:390	emergencies	380:390	Chitosan (CS) has been widely used as a popular hemostatic dressing; however, irregularly shaped wounds present in emergencies limit the performance of CS powder.
32255048	7	101	theme	artery	1217:1222	arg1	model					1235:1239	a femoral artery hemorrhage model	1207:1239	a femoral artery hemorrhage model	1207:1239	In vivo, the hemostatic effects were evaluated in Sprague-Dawley rats and Bama miniature pigs in a femoral artery hemorrhage model or gunshot wound experiment.
32255048	8	102	theme	water-triggered	1320:1334	arg1	swelling					1336:1343	rapid water-triggered swelling	1314:1343	rapid water-triggered swelling	1314:1343	PVA-CS presents robust mechanical strength, rapid water-triggered swelling and a fast absorption speed.
32255048	11	103	theme	enhanced	1784:1791	arg1	healing					1799:1805	enhanced wound healing	1784:1805	enhanced wound healing	1784:1805	Furthermore, in vivo evaluation of the PVA-CS sponges revealed excellent hemostatic performance and enhanced wound healing with increased re-epithelialization and decreased granulation tissues.
31054011	1	0	with	modification	220:231	arg1	chitosan					274:281	chitosan	274:281	chitosan (CTS)	274:287	This study aims to investigate whether modification of solid lipid nanoparticles (SLNs) with chitosan (CTS) and polyethylene glycol (PEG) coatings enhances corneal retention time and transcorneal bioavailability.
31054011	1	0	with	modification	220:231	arg1	PEG					314:316	PEG	314:316	PEG	314:316	This study aims to investigate whether modification of solid lipid nanoparticles (SLNs) with chitosan (CTS) and polyethylene glycol (PEG) coatings enhances corneal retention time and transcorneal bioavailability.
31054011	1	0	with	modification	220:231	arg1	glycol					306:311	polyethylene glycol	293:311	polyethylene glycol (PEG)	293:317	This study aims to investigate whether modification of solid lipid nanoparticles (SLNs) with chitosan (CTS) and polyethylene glycol (PEG) coatings enhances corneal retention time and transcorneal bioavailability.
31054011	1	0	with	modification	220:231	arg1	CTS					284:286	CTS	284:286	CTS	284:286	This study aims to investigate whether modification of solid lipid nanoparticles (SLNs) with chitosan (CTS) and polyethylene glycol (PEG) coatings enhances corneal retention time and transcorneal bioavailability.
31054011	0	1	theme	Chitosan	163:170	arg1	Influence					135:143	the Influence	131:143	Development, Optimization, and In Vitro/In Vivo Characterization of Enhanced Lipid Nanoparticles for Ocular Delivery of Ofloxacin: the Influence of Pegylation and Chitosan Coating.	0:179	Development, Optimization, and In Vitro/In Vivo Characterization of Enhanced Lipid Nanoparticles for Ocular Delivery of Ofloxacin: the Influence of Pegylation and Chitosan Coating.
31054011	1	2	theme	nanoparticles	248:260	arg1	modification					220:231	modification	220:231	modification of solid lipid nanoparticles (SLNs) with chitosan (CTS) and polyethylene glycol (PEG) coatings	220:326	This study aims to investigate whether modification of solid lipid nanoparticles (SLNs) with chitosan (CTS) and polyethylene glycol (PEG) coatings enhances corneal retention time and transcorneal bioavailability.
31054011	9	3	from	concentrations	1751:1764	arg1	eyes					1773:1776	the eyes	1769:1776	the eyes of rabbits	1769:1787	The %OFLOX released from the optimized nanoparticles was 63.3, and 66% of the drug permeated after 24 h. Compared to Oflox® drops, the optimized OFLOX-CTS-PEG-SLN exhibited similar tolerability but two- to threefold higher concentrations in the eyes of rabbits.
31054011	9	4	dep	two-	1726:1729	arg1	to					1731:1732	to	1731:1732	to	1731:1732	The %OFLOX released from the optimized nanoparticles was 63.3, and 66% of the drug permeated after 24 h. Compared to Oflox® drops, the optimized OFLOX-CTS-PEG-SLN exhibited similar tolerability but two- to threefold higher concentrations in the eyes of rabbits.
31054011	4	5	theme	transcorneal	826:837	arg1	permeation					839:848	transcorneal permeation	826:848	transcorneal permeation	826:848	A central composite design was implemented to investigate the influence of total lipid/drug ratio, surfactant concentration, PEG stearate concentration in the lipid mixture, and CTS concentration on size, entrapment, transcorneal permeation, and adhesion to the corneal mucosal membrane.
31054011	6	6	theme	nanoparticle	1080:1091	arg1	morphology					1093:1102	nanoparticle morphology	1080:1102	nanoparticle morphology	1080:1102	Moreover, nanoparticle morphology, eye irritation via histopathological analysis, and OFLOX concentration in the ocular fluids and tissues were determined.
31054011	10	7	theme	ocular	1840:1845	arg1	bioavailability					1847:1861	the ocular bioavailability	1836:1861	the ocular bioavailability of OFLOX	1836:1870	Coating of SLN with chitosan and PEG augments the ocular bioavailability of OFLOX by increasing transcorneal permeation and enhancing mucoadhesion strength.
31054011	7	8	theme	132.9 nm	1389:1396	arg1	particles					1398:1406	132.9 nm particles	1389:1406	132.9 nm particles entrapping 74.8% of the total drug added	1389:1447	A total lipid/drug ratio of 19:1, Tween 80 of 2%, PEG stearate concentration in the lipid mixture (% w/w) of 2.6%, and CTS concentration (% w/v) of 0.23% produced 132.9 nm particles entrapping 74.8% of the total drug added.
31054011	7	9	theme	%	1364:1364	arg1	concentration					1349:1361	CTS concentration	1345:1361	CTS concentration (% w/v) of 0.23%	1345:1378	A total lipid/drug ratio of 19:1, Tween 80 of 2%, PEG stearate concentration in the lipid mixture (% w/w) of 2.6%, and CTS concentration (% w/v) of 0.23% produced 132.9 nm particles entrapping 74.8% of the total drug added.
31054011	7	9	theme	%	1364:1364	arg1	w/v					1366:1368	% w/v	1364:1368	% w/v	1364:1368	A total lipid/drug ratio of 19:1, Tween 80 of 2%, PEG stearate concentration in the lipid mixture (% w/w) of 2.6%, and CTS concentration (% w/v) of 0.23% produced 132.9 nm particles entrapping 74.8% of the total drug added.
31054011	10	10	theme	transcorneal	1886:1897	arg1	permeation					1899:1908	transcorneal permeation	1886:1908	transcorneal permeation	1886:1908	Coating of SLN with chitosan and PEG augments the ocular bioavailability of OFLOX by increasing transcorneal permeation and enhancing mucoadhesion strength.
31054011	4	11	theme	composite	619:627	arg1	design					629:634	A central composite design	609:634	A central composite design	609:634	A central composite design was implemented to investigate the influence of total lipid/drug ratio, surfactant concentration, PEG stearate concentration in the lipid mixture, and CTS concentration on size, entrapment, transcorneal permeation, and adhesion to the corneal mucosal membrane.
31054011	5	12	theme	optimized	901:909	arg1	OFLOX-CTS-PEG-SLN					911:927	The optimized OFLOX-CTS-PEG-SLN	897:927	The optimized OFLOX-CTS-PEG-SLN	897:927	The optimized OFLOX-CTS-PEG-SLN was characterized for OFLOX cumulative percentage released in simulated tear fluid and permeated across the excised bovine corneal membrane.
31054011	2	13	theme	eye	504:506	arg1	infections					508:517	local eye infections	498:517	local eye infections	498:517	Ofloxacin (OFLOX) was selected as the model drug because of its potential benefits for the treatment of local eye infections.
31054011	9	14	attach	released	1539:1546	arg1	nanoparticles					1567:1579	the optimized nanoparticles	1553:1579	the optimized nanoparticles	1553:1579	The %OFLOX released from the optimized nanoparticles was 63.3, and 66% of the drug permeated after 24 h. Compared to Oflox® drops, the optimized OFLOX-CTS-PEG-SLN exhibited similar tolerability but two- to threefold higher concentrations in the eyes of rabbits.
31054011	9	14	attach	released	1539:1546	arg2	OFLOX					1533:1537	The %OFLOX	1528:1537	The %OFLOX released from the optimized nanoparticles	1528:1579	The %OFLOX released from the optimized nanoparticles was 63.3, and 66% of the drug permeated after 24 h. Compared to Oflox® drops, the optimized OFLOX-CTS-PEG-SLN exhibited similar tolerability but two- to threefold higher concentrations in the eyes of rabbits.
31054011	9	14	attach	released	1539:1546	arg2	63.3					1585:1588	63.3	1585:1588	63.3	1585:1588	The %OFLOX released from the optimized nanoparticles was 63.3, and 66% of the drug permeated after 24 h. Compared to Oflox® drops, the optimized OFLOX-CTS-PEG-SLN exhibited similar tolerability but two- to threefold higher concentrations in the eyes of rabbits.
31054011	10	15	theme	mucoadhesion	1924:1935	arg1	strength					1937:1944	mucoadhesion strength	1924:1944	mucoadhesion strength	1924:1944	Coating of SLN with chitosan and PEG augments the ocular bioavailability of OFLOX by increasing transcorneal permeation and enhancing mucoadhesion strength.
31054011	1	16	theme	transcorneal	364:375	arg1	bioavailability					377:391	transcorneal bioavailability	364:391	transcorneal bioavailability	364:391	This study aims to investigate whether modification of solid lipid nanoparticles (SLNs) with chitosan (CTS) and polyethylene glycol (PEG) coatings enhances corneal retention time and transcorneal bioavailability.
31054011	3	17	theme	emulsion/solvent	569:584	arg1	technique					598:606	a modified emulsion/solvent evaporation technique	558:606	a modified emulsion/solvent evaporation technique	558:606	The OFLOX-CTS-PEG-SLN was prepared by a modified emulsion/solvent evaporation technique.
31054011	4	18	theme	mucosal	879:885	arg1	membrane					887:894	the corneal mucosal membrane	867:894	the corneal mucosal membrane	867:894	A central composite design was implemented to investigate the influence of total lipid/drug ratio, surfactant concentration, PEG stearate concentration in the lipid mixture, and CTS concentration on size, entrapment, transcorneal permeation, and adhesion to the corneal mucosal membrane.
31054011	7	19	theme	%	1338:1338	arg1	mixture					1316:1322	the lipid mixture	1306:1322	the lipid mixture (% w/w) of 2.6%	1306:1338	A total lipid/drug ratio of 19:1, Tween 80 of 2%, PEG stearate concentration in the lipid mixture (% w/w) of 2.6%, and CTS concentration (% w/v) of 0.23% produced 132.9 nm particles entrapping 74.8% of the total drug added.
31054011	7	19	theme	%	1338:1338	arg1	w/w					1327:1329	% w/w	1325:1329	% w/w	1325:1329	A total lipid/drug ratio of 19:1, Tween 80 of 2%, PEG stearate concentration in the lipid mixture (% w/w) of 2.6%, and CTS concentration (% w/v) of 0.23% produced 132.9 nm particles entrapping 74.8% of the total drug added.
31054011	5	20	theme	corneal	1052:1058	arg1	membrane					1060:1067	the excised bovine corneal membrane	1033:1067	the excised bovine corneal membrane	1033:1067	The optimized OFLOX-CTS-PEG-SLN was characterized for OFLOX cumulative percentage released in simulated tear fluid and permeated across the excised bovine corneal membrane.
31054011	0	21	dep	Pegylation	148:157	arg1	Coating					172:178	Coating	172:178	Coating	172:178	Development, Optimization, and In Vitro/In Vivo Characterization of Enhanced Lipid Nanoparticles for Ocular Delivery of Ofloxacin: the Influence of Pegylation and Chitosan Coating.
31054011	7	22	from	concentration	1349:1361	arg1	mixture					1316:1322	the lipid mixture	1306:1322	the lipid mixture (% w/w) of 2.6%	1306:1338	A total lipid/drug ratio of 19:1, Tween 80 of 2%, PEG stearate concentration in the lipid mixture (% w/w) of 2.6%, and CTS concentration (% w/v) of 0.23% produced 132.9 nm particles entrapping 74.8% of the total drug added.
31054011	7	22	from	concentration	1349:1361	arg1	w/w					1327:1329	% w/w	1325:1329	% w/w	1325:1329	A total lipid/drug ratio of 19:1, Tween 80 of 2%, PEG stearate concentration in the lipid mixture (% w/w) of 2.6%, and CTS concentration (% w/v) of 0.23% produced 132.9 nm particles entrapping 74.8% of the total drug added.
31054011	7	23	from	ratio	1245:1249	arg1	mixture					1316:1322	the lipid mixture	1306:1322	the lipid mixture (% w/w) of 2.6%	1306:1338	A total lipid/drug ratio of 19:1, Tween 80 of 2%, PEG stearate concentration in the lipid mixture (% w/w) of 2.6%, and CTS concentration (% w/v) of 0.23% produced 132.9 nm particles entrapping 74.8% of the total drug added.
31054011	7	23	from	ratio	1245:1249	arg1	w/w					1327:1329	% w/w	1325:1329	% w/w	1325:1329	A total lipid/drug ratio of 19:1, Tween 80 of 2%, PEG stearate concentration in the lipid mixture (% w/w) of 2.6%, and CTS concentration (% w/v) of 0.23% produced 132.9 nm particles entrapping 74.8% of the total drug added.
31054011	4	24	theme	concentration	719:731	arg1	influence					671:679	the influence	667:679	the influence of total lipid/drug ratio, surfactant concentration, PEG stearate concentration in the lipid mixture, and CTS concentration on size, entrapment, transcorneal permeation, and adhesion to the corneal mucosal membrane	667:894	A central composite design was implemented to investigate the influence of total lipid/drug ratio, surfactant concentration, PEG stearate concentration in the lipid mixture, and CTS concentration on size, entrapment, transcorneal permeation, and adhesion to the corneal mucosal membrane.
31054011	0	25	theme	Pegylation	148:157	arg1	Influence					135:143	the Influence	131:143	Development, Optimization, and In Vitro/In Vivo Characterization of Enhanced Lipid Nanoparticles for Ocular Delivery of Ofloxacin: the Influence of Pegylation and Chitosan Coating.	0:179	Development, Optimization, and In Vitro/In Vivo Characterization of Enhanced Lipid Nanoparticles for Ocular Delivery of Ofloxacin: the Influence of Pegylation and Chitosan Coating.
31054011	4	26	from	concentration	791:803	arg1	mixture					774:780	the lipid mixture	764:780	the lipid mixture	764:780	A central composite design was implemented to investigate the influence of total lipid/drug ratio, surfactant concentration, PEG stearate concentration in the lipid mixture, and CTS concentration on size, entrapment, transcorneal permeation, and adhesion to the corneal mucosal membrane.
31054011	7	27	theme	19:1	1254:1257	arg1	ratio					1245:1249	A total lipid/drug ratio	1226:1249	A total lipid/drug ratio of 19:1	1226:1257	A total lipid/drug ratio of 19:1, Tween 80 of 2%, PEG stearate concentration in the lipid mixture (% w/w) of 2.6%, and CTS concentration (% w/v) of 0.23% produced 132.9 nm particles entrapping 74.8% of the total drug added.
31054011	7	27	theme	19:1	1254:1257	arg1	concentration					1349:1361	CTS concentration	1345:1361	CTS concentration (% w/v) of 0.23%	1345:1378	A total lipid/drug ratio of 19:1, Tween 80 of 2%, PEG stearate concentration in the lipid mixture (% w/w) of 2.6%, and CTS concentration (% w/v) of 0.23% produced 132.9 nm particles entrapping 74.8% of the total drug added.
31054011	7	27	theme	19:1	1254:1257	arg1	w/v					1366:1368	% w/v	1364:1368	% w/v	1364:1368	A total lipid/drug ratio of 19:1, Tween 80 of 2%, PEG stearate concentration in the lipid mixture (% w/w) of 2.6%, and CTS concentration (% w/v) of 0.23% produced 132.9 nm particles entrapping 74.8% of the total drug added.
31054011	7	27	theme	19:1	1254:1257	arg1	concentration					1289:1301	PEG stearate concentration	1276:1301	PEG stearate concentration in the lipid mixture (% w/w) of 2.6%	1276:1338	A total lipid/drug ratio of 19:1, Tween 80 of 2%, PEG stearate concentration in the lipid mixture (% w/w) of 2.6%, and CTS concentration (% w/v) of 0.23% produced 132.9 nm particles entrapping 74.8% of the total drug added.
31054011	4	28	theme	lipid/drug	690:699	arg1	ratio					701:705	total lipid/drug ratio	684:705	total lipid/drug ratio	684:705	A central composite design was implemented to investigate the influence of total lipid/drug ratio, surfactant concentration, PEG stearate concentration in the lipid mixture, and CTS concentration on size, entrapment, transcorneal permeation, and adhesion to the corneal mucosal membrane.
31054011	0	29	theme	Enhanced	68:75	arg1	Nanoparticles					83:95	Enhanced Lipid Nanoparticles	68:95	Enhanced Lipid Nanoparticles for Ocular Delivery of Ofloxacin	68:128	Development, Optimization, and In Vitro/In Vivo Characterization of Enhanced Lipid Nanoparticles for Ocular Delivery of Ofloxacin: the Influence of Pegylation and Chitosan Coating.
31054011	4	30	from	mixture	774:780	arg1	influence					671:679	the influence	667:679	the influence of total lipid/drug ratio, surfactant concentration, PEG stearate concentration in the lipid mixture, and CTS concentration on size, entrapment, transcorneal permeation, and adhesion to the corneal mucosal membrane	667:894	A central composite design was implemented to investigate the influence of total lipid/drug ratio, surfactant concentration, PEG stearate concentration in the lipid mixture, and CTS concentration on size, entrapment, transcorneal permeation, and adhesion to the corneal mucosal membrane.
31054011	0	31	theme	Nanoparticles	83:95	arg1	Optimization					13:24	Optimization	13:24	Optimization	13:24	Development, Optimization, and In Vitro/In Vivo Characterization of Enhanced Lipid Nanoparticles for Ocular Delivery of Ofloxacin: the Influence of Pegylation and Chitosan Coating.
31054011	0	31	theme	Nanoparticles	83:95	arg1	Development					0:10	Development	0:10	Development	0:10	Development, Optimization, and In Vitro/In Vivo Characterization of Enhanced Lipid Nanoparticles for Ocular Delivery of Ofloxacin: the Influence of Pegylation and Chitosan Coating.
31054011	0	31	theme	Nanoparticles	83:95	arg1	Characterization					48:63	In Vitro/In Vivo Characterization	31:63	In Vitro/In Vivo Characterization	31:63	Development, Optimization, and In Vitro/In Vivo Characterization of Enhanced Lipid Nanoparticles for Ocular Delivery of Ofloxacin: the Influence of Pegylation and Chitosan Coating.
31054011	9	32	theme	similar	1701:1707	arg1	tolerability					1709:1720	similar tolerability	1701:1720	similar tolerability	1701:1720	The %OFLOX released from the optimized nanoparticles was 63.3, and 66% of the drug permeated after 24 h. Compared to Oflox® drops, the optimized OFLOX-CTS-PEG-SLN exhibited similar tolerability but two- to threefold higher concentrations in the eyes of rabbits.
31054011	0	33	theme	Ocular	101:106	arg1	Delivery					108:115	Ocular Delivery	101:115	Ocular Delivery of Ofloxacin	101:128	Development, Optimization, and In Vitro/In Vivo Characterization of Enhanced Lipid Nanoparticles for Ocular Delivery of Ofloxacin: the Influence of Pegylation and Chitosan Coating.
31054011	1	34	theme	corneal	337:343	arg1	time					355:358	corneal retention time	337:358	corneal retention time	337:358	This study aims to investigate whether modification of solid lipid nanoparticles (SLNs) with chitosan (CTS) and polyethylene glycol (PEG) coatings enhances corneal retention time and transcorneal bioavailability.
31054011	4	35	from	concentration	747:759	arg1	mixture					774:780	the lipid mixture	764:780	the lipid mixture	764:780	A central composite design was implemented to investigate the influence of total lipid/drug ratio, surfactant concentration, PEG stearate concentration in the lipid mixture, and CTS concentration on size, entrapment, transcorneal permeation, and adhesion to the corneal mucosal membrane.
31054011	5	36	theme	simulated	991:999	arg1	fluid					1006:1010	simulated tear fluid	991:1010	simulated tear fluid	991:1010	The optimized OFLOX-CTS-PEG-SLN was characterized for OFLOX cumulative percentage released in simulated tear fluid and permeated across the excised bovine corneal membrane.
31054011	7	37	theme	%	1325:1325	arg1	mixture					1316:1322	the lipid mixture	1306:1322	the lipid mixture (% w/w) of 2.6%	1306:1338	A total lipid/drug ratio of 19:1, Tween 80 of 2%, PEG stearate concentration in the lipid mixture (% w/w) of 2.6%, and CTS concentration (% w/v) of 0.23% produced 132.9 nm particles entrapping 74.8% of the total drug added.
31054011	7	37	theme	%	1325:1325	arg1	w/w					1327:1329	% w/w	1325:1329	% w/w	1325:1329	A total lipid/drug ratio of 19:1, Tween 80 of 2%, PEG stearate concentration in the lipid mixture (% w/w) of 2.6%, and CTS concentration (% w/v) of 0.23% produced 132.9 nm particles entrapping 74.8% of the total drug added.
31054011	4	38	theme	PEG	734:736	arg1	concentration					747:759	PEG stearate concentration	734:759	PEG stearate concentration in the lipid mixture	734:780	A central composite design was implemented to investigate the influence of total lipid/drug ratio, surfactant concentration, PEG stearate concentration in the lipid mixture, and CTS concentration on size, entrapment, transcorneal permeation, and adhesion to the corneal mucosal membrane.
31054011	4	39	theme	concentration	747:759	arg1	influence					671:679	the influence	667:679	the influence of total lipid/drug ratio, surfactant concentration, PEG stearate concentration in the lipid mixture, and CTS concentration on size, entrapment, transcorneal permeation, and adhesion to the corneal mucosal membrane	667:894	A central composite design was implemented to investigate the influence of total lipid/drug ratio, surfactant concentration, PEG stearate concentration in the lipid mixture, and CTS concentration on size, entrapment, transcorneal permeation, and adhesion to the corneal mucosal membrane.
31054011	6	40	from	concentration	1162:1174	arg1	tissues					1201:1207	tissues	1201:1207	tissues	1201:1207	Moreover, nanoparticle morphology, eye irritation via histopathological analysis, and OFLOX concentration in the ocular fluids and tissues were determined.
31054011	6	40	from	concentration	1162:1174	arg1	fluids					1190:1195	the ocular fluids	1179:1195	the ocular fluids	1179:1195	Moreover, nanoparticle morphology, eye irritation via histopathological analysis, and OFLOX concentration in the ocular fluids and tissues were determined.
31054011	5	41	theme	cumulative	957:966	arg1	percentage					968:977	OFLOX cumulative percentage	951:977	OFLOX cumulative percentage released in simulated tear fluid	951:1010	The optimized OFLOX-CTS-PEG-SLN was characterized for OFLOX cumulative percentage released in simulated tear fluid and permeated across the excised bovine corneal membrane.
31054011	9	42	theme	higher	1744:1749	arg1	concentrations					1751:1764	two- to threefold higher concentrations	1726:1764	two- to threefold higher concentrations in the eyes of rabbits	1726:1787	The %OFLOX released from the optimized nanoparticles was 63.3, and 66% of the drug permeated after 24 h. Compared to Oflox® drops, the optimized OFLOX-CTS-PEG-SLN exhibited similar tolerability but two- to threefold higher concentrations in the eyes of rabbits.
31054011	2	43	theme	model	432:436	arg1	Ofloxacin					394:402	Ofloxacin	394:402	Ofloxacin (OFLOX)	394:410	Ofloxacin (OFLOX) was selected as the model drug because of its potential benefits for the treatment of local eye infections.
31054011	2	43	theme	model	432:436	arg1	drug					438:441	the model drug	428:441	the model drug	428:441	Ofloxacin (OFLOX) was selected as the model drug because of its potential benefits for the treatment of local eye infections.
31054011	5	44	theme	bovine	1045:1050	arg1	membrane					1060:1067	the excised bovine corneal membrane	1033:1067	the excised bovine corneal membrane	1033:1067	The optimized OFLOX-CTS-PEG-SLN was characterized for OFLOX cumulative percentage released in simulated tear fluid and permeated across the excised bovine corneal membrane.
31054011	7	45	theme	PEG	1276:1278	arg1	concentration					1289:1301	PEG stearate concentration	1276:1301	PEG stearate concentration in the lipid mixture (% w/w) of 2.6%	1276:1338	A total lipid/drug ratio of 19:1, Tween 80 of 2%, PEG stearate concentration in the lipid mixture (% w/w) of 2.6%, and CTS concentration (% w/v) of 0.23% produced 132.9 nm particles entrapping 74.8% of the total drug added.
31054011	10	46	theme	SLN	1801:1803	arg1	Coating					1790:1796	Coating	1790:1796	Coating of SLN with chitosan and PEG	1790:1825	Coating of SLN with chitosan and PEG augments the ocular bioavailability of OFLOX by increasing transcorneal permeation and enhancing mucoadhesion strength.
31054011	9	47	theme	optimized	1557:1565	arg1	nanoparticles					1567:1579	the optimized nanoparticles	1553:1579	the optimized nanoparticles	1553:1579	The %OFLOX released from the optimized nanoparticles was 63.3, and 66% of the drug permeated after 24 h. Compared to Oflox® drops, the optimized OFLOX-CTS-PEG-SLN exhibited similar tolerability but two- to threefold higher concentrations in the eyes of rabbits.
31054011	7	48	theme	total	1432:1436	arg1	drug					1438:1441	the total drug	1428:1441	the total drug added	1428:1447	A total lipid/drug ratio of 19:1, Tween 80 of 2%, PEG stearate concentration in the lipid mixture (% w/w) of 2.6%, and CTS concentration (% w/v) of 0.23% produced 132.9 nm particles entrapping 74.8% of the total drug added.
31054011	7	49	theme	%	1273:1273	arg1	ratio					1245:1249	A total lipid/drug ratio	1226:1249	A total lipid/drug ratio of 19:1	1226:1257	A total lipid/drug ratio of 19:1, Tween 80 of 2%, PEG stearate concentration in the lipid mixture (% w/w) of 2.6%, and CTS concentration (% w/v) of 0.23% produced 132.9 nm particles entrapping 74.8% of the total drug added.
31054011	7	49	theme	%	1273:1273	arg1	concentration					1349:1361	CTS concentration	1345:1361	CTS concentration (% w/v) of 0.23%	1345:1378	A total lipid/drug ratio of 19:1, Tween 80 of 2%, PEG stearate concentration in the lipid mixture (% w/w) of 2.6%, and CTS concentration (% w/v) of 0.23% produced 132.9 nm particles entrapping 74.8% of the total drug added.
31054011	7	49	theme	%	1273:1273	arg1	w/v					1366:1368	% w/v	1364:1368	% w/v	1364:1368	A total lipid/drug ratio of 19:1, Tween 80 of 2%, PEG stearate concentration in the lipid mixture (% w/w) of 2.6%, and CTS concentration (% w/v) of 0.23% produced 132.9 nm particles entrapping 74.8% of the total drug added.
31054011	7	49	theme	%	1273:1273	arg1	concentration					1289:1301	PEG stearate concentration	1276:1301	PEG stearate concentration in the lipid mixture (% w/w) of 2.6%	1276:1338	A total lipid/drug ratio of 19:1, Tween 80 of 2%, PEG stearate concentration in the lipid mixture (% w/w) of 2.6%, and CTS concentration (% w/v) of 0.23% produced 132.9 nm particles entrapping 74.8% of the total drug added.
31054011	9	50	theme	rabbits	1781:1787	arg1	eyes					1773:1776	the eyes	1769:1776	the eyes of rabbits	1769:1787	The %OFLOX released from the optimized nanoparticles was 63.3, and 66% of the drug permeated after 24 h. Compared to Oflox® drops, the optimized OFLOX-CTS-PEG-SLN exhibited similar tolerability but two- to threefold higher concentrations in the eyes of rabbits.
31054011	4	51	theme	concentration	791:803	arg1	influence					671:679	the influence	667:679	the influence of total lipid/drug ratio, surfactant concentration, PEG stearate concentration in the lipid mixture, and CTS concentration on size, entrapment, transcorneal permeation, and adhesion to the corneal mucosal membrane	667:894	A central composite design was implemented to investigate the influence of total lipid/drug ratio, surfactant concentration, PEG stearate concentration in the lipid mixture, and CTS concentration on size, entrapment, transcorneal permeation, and adhesion to the corneal mucosal membrane.
31054011	4	52	from	ratio	701:705	arg1	mixture					774:780	the lipid mixture	764:780	the lipid mixture	764:780	A central composite design was implemented to investigate the influence of total lipid/drug ratio, surfactant concentration, PEG stearate concentration in the lipid mixture, and CTS concentration on size, entrapment, transcorneal permeation, and adhesion to the corneal mucosal membrane.
31054011	0	53	dep	Development	0:10	arg1	Influence					135:143	the Influence	131:143	Development, Optimization, and In Vitro/In Vivo Characterization of Enhanced Lipid Nanoparticles for Ocular Delivery of Ofloxacin: the Influence of Pegylation and Chitosan Coating.	0:179	Development, Optimization, and In Vitro/In Vivo Characterization of Enhanced Lipid Nanoparticles for Ocular Delivery of Ofloxacin: the Influence of Pegylation and Chitosan Coating.
31054011	10	54	with	Coating	1790:1796	arg1	PEG					1823:1825	PEG	1823:1825	PEG	1823:1825	Coating of SLN with chitosan and PEG augments the ocular bioavailability of OFLOX by increasing transcorneal permeation and enhancing mucoadhesion strength.
31054011	10	54	with	Coating	1790:1796	arg1	chitosan					1810:1817	chitosan	1810:1817	chitosan	1810:1817	Coating of SLN with chitosan and PEG augments the ocular bioavailability of OFLOX by increasing transcorneal permeation and enhancing mucoadhesion strength.
31054011	7	55	theme	lipid/drug	1234:1243	arg1	ratio					1245:1249	A total lipid/drug ratio	1226:1249	A total lipid/drug ratio of 19:1	1226:1257	A total lipid/drug ratio of 19:1, Tween 80 of 2%, PEG stearate concentration in the lipid mixture (% w/w) of 2.6%, and CTS concentration (% w/v) of 0.23% produced 132.9 nm particles entrapping 74.8% of the total drug added.
31054011	1	56	theme	lipid	242:246	arg1	nanoparticles					248:260	solid lipid nanoparticles	236:260	solid lipid nanoparticles (SLNs)	236:267	This study aims to investigate whether modification of solid lipid nanoparticles (SLNs) with chitosan (CTS) and polyethylene glycol (PEG) coatings enhances corneal retention time and transcorneal bioavailability.
31054011	1	56	theme	lipid	242:246	arg1	SLNs					263:266	SLNs	263:266	SLNs	263:266	This study aims to investigate whether modification of solid lipid nanoparticles (SLNs) with chitosan (CTS) and polyethylene glycol (PEG) coatings enhances corneal retention time and transcorneal bioavailability.
31054011	6	57	theme	eye	1105:1107	arg1	irritation					1109:1118	eye irritation	1105:1118	eye irritation via histopathological analysis	1105:1149	Moreover, nanoparticle morphology, eye irritation via histopathological analysis, and OFLOX concentration in the ocular fluids and tissues were determined.
31054011	0	58	dep	In	31:32	arg1	Vivo					43:46	Vivo	43:46	Vivo	43:46	Development, Optimization, and In Vitro/In Vivo Characterization of Enhanced Lipid Nanoparticles for Ocular Delivery of Ofloxacin: the Influence of Pegylation and Chitosan Coating.
31054011	4	59	from	influence	671:679	arg1	permeation					839:848	transcorneal permeation	826:848	transcorneal permeation	826:848	A central composite design was implemented to investigate the influence of total lipid/drug ratio, surfactant concentration, PEG stearate concentration in the lipid mixture, and CTS concentration on size, entrapment, transcorneal permeation, and adhesion to the corneal mucosal membrane.
31054011	4	59	from	influence	671:679	arg1	adhesion					855:862	adhesion	855:862	adhesion to the corneal mucosal membrane	855:894	A central composite design was implemented to investigate the influence of total lipid/drug ratio, surfactant concentration, PEG stearate concentration in the lipid mixture, and CTS concentration on size, entrapment, transcorneal permeation, and adhesion to the corneal mucosal membrane.
31054011	4	59	from	influence	671:679	arg1	size					808:811	size	808:811	size	808:811	A central composite design was implemented to investigate the influence of total lipid/drug ratio, surfactant concentration, PEG stearate concentration in the lipid mixture, and CTS concentration on size, entrapment, transcorneal permeation, and adhesion to the corneal mucosal membrane.
31054011	4	59	from	influence	671:679	arg1	entrapment					814:823	entrapment	814:823	entrapment	814:823	A central composite design was implemented to investigate the influence of total lipid/drug ratio, surfactant concentration, PEG stearate concentration in the lipid mixture, and CTS concentration on size, entrapment, transcorneal permeation, and adhesion to the corneal mucosal membrane.
31054011	4	59	from	influence	671:679	arg1	mixture					774:780	the lipid mixture	764:780	the lipid mixture	764:780	A central composite design was implemented to investigate the influence of total lipid/drug ratio, surfactant concentration, PEG stearate concentration in the lipid mixture, and CTS concentration on size, entrapment, transcorneal permeation, and adhesion to the corneal mucosal membrane.
31054011	6	60	theme	ocular	1183:1188	arg1	fluids					1190:1195	the ocular fluids	1179:1195	the ocular fluids	1179:1195	Moreover, nanoparticle morphology, eye irritation via histopathological analysis, and OFLOX concentration in the ocular fluids and tissues were determined.
31054011	7	61	theme	%	1378:1378	arg1	ratio					1245:1249	A total lipid/drug ratio	1226:1249	A total lipid/drug ratio of 19:1	1226:1257	A total lipid/drug ratio of 19:1, Tween 80 of 2%, PEG stearate concentration in the lipid mixture (% w/w) of 2.6%, and CTS concentration (% w/v) of 0.23% produced 132.9 nm particles entrapping 74.8% of the total drug added.
31054011	7	61	theme	%	1378:1378	arg1	concentration					1349:1361	CTS concentration	1345:1361	CTS concentration (% w/v) of 0.23%	1345:1378	A total lipid/drug ratio of 19:1, Tween 80 of 2%, PEG stearate concentration in the lipid mixture (% w/w) of 2.6%, and CTS concentration (% w/v) of 0.23% produced 132.9 nm particles entrapping 74.8% of the total drug added.
31054011	7	61	theme	%	1378:1378	arg1	w/v					1366:1368	% w/v	1364:1368	% w/v	1364:1368	A total lipid/drug ratio of 19:1, Tween 80 of 2%, PEG stearate concentration in the lipid mixture (% w/w) of 2.6%, and CTS concentration (% w/v) of 0.23% produced 132.9 nm particles entrapping 74.8% of the total drug added.
31054011	7	61	theme	%	1378:1378	arg1	concentration					1289:1301	PEG stearate concentration	1276:1301	PEG stearate concentration in the lipid mixture (% w/w) of 2.6%	1276:1338	A total lipid/drug ratio of 19:1, Tween 80 of 2%, PEG stearate concentration in the lipid mixture (% w/w) of 2.6%, and CTS concentration (% w/v) of 0.23% produced 132.9 nm particles entrapping 74.8% of the total drug added.
31054011	6	62	from	morphology	1093:1102	arg1	tissues					1201:1207	tissues	1201:1207	tissues	1201:1207	Moreover, nanoparticle morphology, eye irritation via histopathological analysis, and OFLOX concentration in the ocular fluids and tissues were determined.
31054011	6	62	from	morphology	1093:1102	arg1	fluids					1190:1195	the ocular fluids	1179:1195	the ocular fluids	1179:1195	Moreover, nanoparticle morphology, eye irritation via histopathological analysis, and OFLOX concentration in the ocular fluids and tissues were determined.
31054011	9	63	theme	optimized	1663:1671	arg1	OFLOX-CTS-PEG-SLN					1673:1689	the optimized OFLOX-CTS-PEG-SLN	1659:1689	the optimized OFLOX-CTS-PEG-SLN	1659:1689	The %OFLOX released from the optimized nanoparticles was 63.3, and 66% of the drug permeated after 24 h. Compared to Oflox® drops, the optimized OFLOX-CTS-PEG-SLN exhibited similar tolerability but two- to threefold higher concentrations in the eyes of rabbits.
31054011	0	64	theme	Ofloxacin	120:128	arg1	Delivery					108:115	Ocular Delivery	101:115	Ocular Delivery of Ofloxacin	101:128	Development, Optimization, and In Vitro/In Vivo Characterization of Enhanced Lipid Nanoparticles for Ocular Delivery of Ofloxacin: the Influence of Pegylation and Chitosan Coating.
31054011	2	65	theme	infections	508:517	arg1	treatment					485:493	the treatment	481:493	the treatment of local eye infections	481:517	Ofloxacin (OFLOX) was selected as the model drug because of its potential benefits for the treatment of local eye infections.
31054011	4	66	theme	central	611:617	arg1	design					629:634	A central composite design	609:634	A central composite design	609:634	A central composite design was implemented to investigate the influence of total lipid/drug ratio, surfactant concentration, PEG stearate concentration in the lipid mixture, and CTS concentration on size, entrapment, transcorneal permeation, and adhesion to the corneal mucosal membrane.
31054011	10	67	theme	OFLOX	1866:1870	arg1	bioavailability					1847:1861	the ocular bioavailability	1836:1861	the ocular bioavailability of OFLOX	1836:1870	Coating of SLN with chitosan and PEG augments the ocular bioavailability of OFLOX by increasing transcorneal permeation and enhancing mucoadhesion strength.
31054011	3	68	theme	modified	560:567	arg1	technique					598:606	a modified emulsion/solvent evaporation technique	558:606	a modified emulsion/solvent evaporation technique	558:606	The OFLOX-CTS-PEG-SLN was prepared by a modified emulsion/solvent evaporation technique.
31054011	2	69	theme	local	498:502	arg1	infections					508:517	local eye infections	498:517	local eye infections	498:517	Ofloxacin (OFLOX) was selected as the model drug because of its potential benefits for the treatment of local eye infections.
31054011	7	70	theme	CTS	1345:1347	arg1	concentration					1349:1361	CTS concentration	1345:1361	CTS concentration (% w/v) of 0.23%	1345:1378	A total lipid/drug ratio of 19:1, Tween 80 of 2%, PEG stearate concentration in the lipid mixture (% w/w) of 2.6%, and CTS concentration (% w/v) of 0.23% produced 132.9 nm particles entrapping 74.8% of the total drug added.
31054011	7	70	theme	CTS	1345:1347	arg1	w/v					1366:1368	% w/v	1364:1368	% w/v	1364:1368	A total lipid/drug ratio of 19:1, Tween 80 of 2%, PEG stearate concentration in the lipid mixture (% w/w) of 2.6%, and CTS concentration (% w/v) of 0.23% produced 132.9 nm particles entrapping 74.8% of the total drug added.
31054011	4	71	theme	corneal	871:877	arg1	membrane					887:894	the corneal mucosal membrane	867:894	the corneal mucosal membrane	867:894	A central composite design was implemented to investigate the influence of total lipid/drug ratio, surfactant concentration, PEG stearate concentration in the lipid mixture, and CTS concentration on size, entrapment, transcorneal permeation, and adhesion to the corneal mucosal membrane.
31054011	4	72	theme	ratio	701:705	arg1	influence					671:679	the influence	667:679	the influence of total lipid/drug ratio, surfactant concentration, PEG stearate concentration in the lipid mixture, and CTS concentration on size, entrapment, transcorneal permeation, and adhesion to the corneal mucosal membrane	667:894	A central composite design was implemented to investigate the influence of total lipid/drug ratio, surfactant concentration, PEG stearate concentration in the lipid mixture, and CTS concentration on size, entrapment, transcorneal permeation, and adhesion to the corneal mucosal membrane.
31054011	9	73	theme	Oflox®	1645:1650	arg1	drops					1652:1656	Oflox® drops	1645:1656	Oflox® drops	1645:1656	The %OFLOX released from the optimized nanoparticles was 63.3, and 66% of the drug permeated after 24 h. Compared to Oflox® drops, the optimized OFLOX-CTS-PEG-SLN exhibited similar tolerability but two- to threefold higher concentrations in the eyes of rabbits.
31054011	4	74	theme	surfactant	708:717	arg1	concentration					719:731	surfactant concentration	708:731	surfactant concentration	708:731	A central composite design was implemented to investigate the influence of total lipid/drug ratio, surfactant concentration, PEG stearate concentration in the lipid mixture, and CTS concentration on size, entrapment, transcorneal permeation, and adhesion to the corneal mucosal membrane.
31054011	3	75	theme	evaporation	586:596	arg1	technique					598:606	a modified emulsion/solvent evaporation technique	558:606	a modified emulsion/solvent evaporation technique	558:606	The OFLOX-CTS-PEG-SLN was prepared by a modified emulsion/solvent evaporation technique.
31054011	2	76	theme	potential	458:466	arg1	benefits					468:475	its potential benefits	454:475	its potential benefits for the treatment of local eye infections	454:517	Ofloxacin (OFLOX) was selected as the model drug because of its potential benefits for the treatment of local eye infections.
31054011	1	77	dep	chitosan	274:281	arg1	coatings					319:326	coatings	319:326	coatings	319:326	This study aims to investigate whether modification of solid lipid nanoparticles (SLNs) with chitosan (CTS) and polyethylene glycol (PEG) coatings enhances corneal retention time and transcorneal bioavailability.
31054011	7	78	theme	total	1228:1232	arg1	ratio					1245:1249	A total lipid/drug ratio	1226:1249	A total lipid/drug ratio of 19:1	1226:1257	A total lipid/drug ratio of 19:1, Tween 80 of 2%, PEG stearate concentration in the lipid mixture (% w/w) of 2.6%, and CTS concentration (% w/v) of 0.23% produced 132.9 nm particles entrapping 74.8% of the total drug added.
31054011	0	79	theme	Lipid	77:81	arg1	Nanoparticles					83:95	Enhanced Lipid Nanoparticles	68:95	Enhanced Lipid Nanoparticles for Ocular Delivery of Ofloxacin	68:128	Development, Optimization, and In Vitro/In Vivo Characterization of Enhanced Lipid Nanoparticles for Ocular Delivery of Ofloxacin: the Influence of Pegylation and Chitosan Coating.
31054011	9	80	theme	drug	1606:1609	arg1	%					1597:1597	66%	1595:1597	66% of the drug	1595:1609	The %OFLOX released from the optimized nanoparticles was 63.3, and 66% of the drug permeated after 24 h. Compared to Oflox® drops, the optimized OFLOX-CTS-PEG-SLN exhibited similar tolerability but two- to threefold higher concentrations in the eyes of rabbits.
31054011	9	80	theme	drug	1606:1609	arg1	drug					1606:1609	the drug	1602:1609	the drug	1602:1609	The %OFLOX released from the optimized nanoparticles was 63.3, and 66% of the drug permeated after 24 h. Compared to Oflox® drops, the optimized OFLOX-CTS-PEG-SLN exhibited similar tolerability but two- to threefold higher concentrations in the eyes of rabbits.
31054011	4	81	theme	total	684:688	arg1	ratio					701:705	total lipid/drug ratio	684:705	total lipid/drug ratio	684:705	A central composite design was implemented to investigate the influence of total lipid/drug ratio, surfactant concentration, PEG stearate concentration in the lipid mixture, and CTS concentration on size, entrapment, transcorneal permeation, and adhesion to the corneal mucosal membrane.
31054011	4	82	theme	lipid	768:772	arg1	mixture					774:780	the lipid mixture	764:780	the lipid mixture	764:780	A central composite design was implemented to investigate the influence of total lipid/drug ratio, surfactant concentration, PEG stearate concentration in the lipid mixture, and CTS concentration on size, entrapment, transcorneal permeation, and adhesion to the corneal mucosal membrane.
31054011	1	83	theme	retention	345:353	arg1	time					355:358	corneal retention time	337:358	corneal retention time	337:358	This study aims to investigate whether modification of solid lipid nanoparticles (SLNs) with chitosan (CTS) and polyethylene glycol (PEG) coatings enhances corneal retention time and transcorneal bioavailability.
31054011	7	84	theme	lipid	1310:1314	arg1	mixture					1316:1322	the lipid mixture	1306:1322	the lipid mixture (% w/w) of 2.6%	1306:1338	A total lipid/drug ratio of 19:1, Tween 80 of 2%, PEG stearate concentration in the lipid mixture (% w/w) of 2.6%, and CTS concentration (% w/v) of 0.23% produced 132.9 nm particles entrapping 74.8% of the total drug added.
31054011	7	84	theme	lipid	1310:1314	arg1	w/w					1327:1329	% w/w	1325:1329	% w/w	1325:1329	A total lipid/drug ratio of 19:1, Tween 80 of 2%, PEG stearate concentration in the lipid mixture (% w/w) of 2.6%, and CTS concentration (% w/v) of 0.23% produced 132.9 nm particles entrapping 74.8% of the total drug added.
31054011	8	85	theme	corneal	1482:1488	arg1	membrane					1490:1497	the corneal membrane	1478:1497	the corneal membrane	1478:1497	The particles detached from the corneal membrane at a force of 3700 dyne/cm2.
31054011	5	86	theme	OFLOX	951:955	arg1	percentage					968:977	OFLOX cumulative percentage	951:977	OFLOX cumulative percentage released in simulated tear fluid	951:1010	The optimized OFLOX-CTS-PEG-SLN was characterized for OFLOX cumulative percentage released in simulated tear fluid and permeated across the excised bovine corneal membrane.
31054011	4	87	theme	stearate	738:745	arg1	concentration					747:759	PEG stearate concentration	734:759	PEG stearate concentration in the lipid mixture	734:780	A central composite design was implemented to investigate the influence of total lipid/drug ratio, surfactant concentration, PEG stearate concentration in the lipid mixture, and CTS concentration on size, entrapment, transcorneal permeation, and adhesion to the corneal mucosal membrane.
31054011	0	88	theme	In	31:32	arg1	Characterization					48:63	In Vitro/In Vivo Characterization	31:63	In Vitro/In Vivo Characterization	31:63	Development, Optimization, and In Vitro/In Vivo Characterization of Enhanced Lipid Nanoparticles for Ocular Delivery of Ofloxacin: the Influence of Pegylation and Chitosan Coating.
31054011	7	89	theme	stearate	1280:1287	arg1	concentration					1289:1301	PEG stearate concentration	1276:1301	PEG stearate concentration in the lipid mixture (% w/w) of 2.6%	1276:1338	A total lipid/drug ratio of 19:1, Tween 80 of 2%, PEG stearate concentration in the lipid mixture (% w/w) of 2.6%, and CTS concentration (% w/v) of 0.23% produced 132.9 nm particles entrapping 74.8% of the total drug added.
31054011	5	90	theme	excised	1037:1043	arg1	membrane					1060:1067	the excised bovine corneal membrane	1033:1067	the excised bovine corneal membrane	1033:1067	The optimized OFLOX-CTS-PEG-SLN was characterized for OFLOX cumulative percentage released in simulated tear fluid and permeated across the excised bovine corneal membrane.
31054011	7	91	from	concentration	1289:1301	arg1	mixture					1316:1322	the lipid mixture	1306:1322	the lipid mixture (% w/w) of 2.6%	1306:1338	A total lipid/drug ratio of 19:1, Tween 80 of 2%, PEG stearate concentration in the lipid mixture (% w/w) of 2.6%, and CTS concentration (% w/v) of 0.23% produced 132.9 nm particles entrapping 74.8% of the total drug added.
31054011	7	91	from	concentration	1289:1301	arg1	w/w					1327:1329	% w/w	1325:1329	% w/w	1325:1329	A total lipid/drug ratio of 19:1, Tween 80 of 2%, PEG stearate concentration in the lipid mixture (% w/w) of 2.6%, and CTS concentration (% w/v) of 0.23% produced 132.9 nm particles entrapping 74.8% of the total drug added.
31054011	6	92	theme	OFLOX	1156:1160	arg1	concentration					1162:1174	OFLOX concentration	1156:1174	OFLOX concentration in the ocular fluids and tissues	1156:1207	Moreover, nanoparticle morphology, eye irritation via histopathological analysis, and OFLOX concentration in the ocular fluids and tissues were determined.
31054011	8	93	theme	3700 dyne/cm2	1513:1525	arg1	force					1504:1508	a force	1502:1508	a force of 3700 dyne/cm2	1502:1525	The particles detached from the corneal membrane at a force of 3700 dyne/cm2.
31054011	4	94	from	concentration	719:731	arg1	mixture					774:780	the lipid mixture	764:780	the lipid mixture	764:780	A central composite design was implemented to investigate the influence of total lipid/drug ratio, surfactant concentration, PEG stearate concentration in the lipid mixture, and CTS concentration on size, entrapment, transcorneal permeation, and adhesion to the corneal mucosal membrane.
31054011	1	95	theme	polyethylene	293:304	arg1	PEG					314:316	PEG	314:316	PEG	314:316	This study aims to investigate whether modification of solid lipid nanoparticles (SLNs) with chitosan (CTS) and polyethylene glycol (PEG) coatings enhances corneal retention time and transcorneal bioavailability.
31054011	1	95	theme	polyethylene	293:304	arg1	glycol					306:311	polyethylene glycol	293:311	polyethylene glycol (PEG)	293:317	This study aims to investigate whether modification of solid lipid nanoparticles (SLNs) with chitosan (CTS) and polyethylene glycol (PEG) coatings enhances corneal retention time and transcorneal bioavailability.
31054011	9	96	theme	%	1532:1532	arg1	63.3					1585:1588	63.3	1585:1588	63.3	1585:1588	The %OFLOX released from the optimized nanoparticles was 63.3, and 66% of the drug permeated after 24 h. Compared to Oflox® drops, the optimized OFLOX-CTS-PEG-SLN exhibited similar tolerability but two- to threefold higher concentrations in the eyes of rabbits.
31054011	9	96	theme	%	1532:1532	arg1	OFLOX					1533:1537	The %OFLOX	1528:1537	The %OFLOX released from the optimized nanoparticles	1528:1579	The %OFLOX released from the optimized nanoparticles was 63.3, and 66% of the drug permeated after 24 h. Compared to Oflox® drops, the optimized OFLOX-CTS-PEG-SLN exhibited similar tolerability but two- to threefold higher concentrations in the eyes of rabbits.
31054011	5	97	theme	tear	1001:1004	arg1	fluid					1006:1010	simulated tear fluid	991:1010	simulated tear fluid	991:1010	The optimized OFLOX-CTS-PEG-SLN was characterized for OFLOX cumulative percentage released in simulated tear fluid and permeated across the excised bovine corneal membrane.
31054011	6	98	from	irritation	1109:1118	arg1	tissues					1201:1207	tissues	1201:1207	tissues	1201:1207	Moreover, nanoparticle morphology, eye irritation via histopathological analysis, and OFLOX concentration in the ocular fluids and tissues were determined.
31054011	6	98	from	irritation	1109:1118	arg1	fluids					1190:1195	the ocular fluids	1179:1195	the ocular fluids	1179:1195	Moreover, nanoparticle morphology, eye irritation via histopathological analysis, and OFLOX concentration in the ocular fluids and tissues were determined.
31054011	7	99	theme	drug	1438:1441	arg1	%					1423:1423	74.8%	1419:1423	74.8% of the total drug added	1419:1447	A total lipid/drug ratio of 19:1, Tween 80 of 2%, PEG stearate concentration in the lipid mixture (% w/w) of 2.6%, and CTS concentration (% w/v) of 0.23% produced 132.9 nm particles entrapping 74.8% of the total drug added.
31054011	7	99	theme	drug	1438:1441	arg1	drug					1438:1441	the total drug	1428:1441	the total drug added	1428:1447	A total lipid/drug ratio of 19:1, Tween 80 of 2%, PEG stearate concentration in the lipid mixture (% w/w) of 2.6%, and CTS concentration (% w/v) of 0.23% produced 132.9 nm particles entrapping 74.8% of the total drug added.
31054011	6	100	theme	histopathological	1124:1140	arg1	analysis					1142:1149	histopathological analysis	1124:1149	histopathological analysis	1124:1149	Moreover, nanoparticle morphology, eye irritation via histopathological analysis, and OFLOX concentration in the ocular fluids and tissues were determined.
31054011	4	101	theme	CTS	787:789	arg1	concentration					791:803	CTS concentration	787:803	CTS concentration	787:803	A central composite design was implemented to investigate the influence of total lipid/drug ratio, surfactant concentration, PEG stearate concentration in the lipid mixture, and CTS concentration on size, entrapment, transcorneal permeation, and adhesion to the corneal mucosal membrane.
31054011	1	102	theme	solid	236:240	arg1	nanoparticles					248:260	solid lipid nanoparticles	236:260	solid lipid nanoparticles (SLNs)	236:267	This study aims to investigate whether modification of solid lipid nanoparticles (SLNs) with chitosan (CTS) and polyethylene glycol (PEG) coatings enhances corneal retention time and transcorneal bioavailability.
31054011	1	102	theme	solid	236:240	arg1	SLNs					263:266	SLNs	263:266	SLNs	263:266	This study aims to investigate whether modification of solid lipid nanoparticles (SLNs) with chitosan (CTS) and polyethylene glycol (PEG) coatings enhances corneal retention time and transcorneal bioavailability.
30166109	17	0	with	pre-treatment	2268:2280	arg1	Pc-EtOH					2287:2293	Pc-EtOH	2287:2293	Pc-EtOH	2287:2293	In the peritonitis test, acute pre-treatment with Pc-EtOH inhibited leukocyte migration, with a reduction in the number of neutrophils and concentration of total proteins and nitric oxide.
30166109	9	1	theme	rota-rod	1140:1147	arg1	test					1149:1152	The rota-rod test	1136:1152	The rota-rod test	1136:1152	The rota-rod test was used to verify the possible interference of the extract treatment in motor performance.
30166109	7	2	theme	abdominal	865:873	arg1	constriction					875:886	acetic acid-induced abdominal constriction	845:886	acetic acid-induced abdominal constriction	845:886	The antinociceptive profile of the extract (given orally: 100, 200 and 400 mg/kg) was established using the in vivo chemical models (acetic acid-induced abdominal constriction and formalin-induced paw licking test) and thermal (hot plate test) of nociception.
30166109	7	2	theme	abdominal	865:873	arg1	models					837:842	the in vivo chemical models	816:842	the in vivo chemical models (acetic acid-induced abdominal constriction and formalin-induced paw licking test)	816:925	The antinociceptive profile of the extract (given orally: 100, 200 and 400 mg/kg) was established using the in vivo chemical models (acetic acid-induced abdominal constriction and formalin-induced paw licking test) and thermal (hot plate test) of nociception.
30166109	14	3	theme	oxide	1953:1957	arg1	pathway					1959:1965	the nitric oxide pathway	1942:1965	the nitric oxide pathway	1942:1965	The antinociceptive mechanism probably involves central and peripheral pathways, involving the pathway of opioid and muscarinic receptors with influence of potassium channels and the nitric oxide pathway.
30166109	2	4	theme	Passiflora	150:159	arg1	Mast					172:175	BACKGROUND Passiflora cincinnata Mast	139:175	BACKGROUND Passiflora cincinnata Mast.	139:176	BACKGROUND Passiflora cincinnata Mast.
30166109	7	5	theme	acetic	845:850	arg1	constriction					875:886	acetic acid-induced abdominal constriction	845:886	acetic acid-induced abdominal constriction	845:886	The antinociceptive profile of the extract (given orally: 100, 200 and 400 mg/kg) was established using the in vivo chemical models (acetic acid-induced abdominal constriction and formalin-induced paw licking test) and thermal (hot plate test) of nociception.
30166109	7	5	theme	acetic	845:850	arg1	models					837:842	the in vivo chemical models	816:842	the in vivo chemical models (acetic acid-induced abdominal constriction and formalin-induced paw licking test)	816:925	The antinociceptive profile of the extract (given orally: 100, 200 and 400 mg/kg) was established using the in vivo chemical models (acetic acid-induced abdominal constriction and formalin-induced paw licking test) and thermal (hot plate test) of nociception.
30166109	9	6	from	interference	1186:1197	arg1	performance					1233:1243	motor performance	1227:1243	motor performance	1227:1243	The rota-rod test was used to verify the possible interference of the extract treatment in motor performance.
30166109	18	7	theme	antinociceptive	2510:2524	arg1	action					2526:2531	peripheral and central antinociceptive action	2487:2531	peripheral and central antinociceptive action	2487:2531	CONCLUSION The present study suggests that Pc-EtOH possesses peripheral and central antinociceptive action, and showed potential in inhibition of release of mediators of the inflammatory process.
30166109	5	8	theme	parts	519:523	arg1	activities					494:503	the antinociceptive and anti-inflammatory activities	452:503	the antinociceptive and anti-inflammatory activities of the aerial parts of Passiflora cincinnata (Pc-EtOH) in mice	452:566	HYPOTHESIS/PURPOSE This paper aims to evaluate the antinociceptive and anti-inflammatory activities of the aerial parts of Passiflora cincinnata (Pc-EtOH) in mice.
30166109	17	9	theme	total	2393:2397	arg1	proteins					2399:2406	total proteins	2393:2406	total proteins	2393:2406	In the peritonitis test, acute pre-treatment with Pc-EtOH inhibited leukocyte migration, with a reduction in the number of neutrophils and concentration of total proteins and nitric oxide.
30166109	16	10	theme	histamine	2226:2234	arg1	administration					2192:2205	administration	2192:2205	administration of carrageenan and histamine	2192:2234	Pc-EtOH inhibited significantly (p < 0.05) the increase of the edema volume after administration of carrageenan and histamine.
30166109	7	11	theme	antinociceptive	716:730	arg1	profile					732:738	The antinociceptive profile	712:738	The antinociceptive profile of the extract (given orally: 100, 200 and 400 mg/kg)	712:792	The antinociceptive profile of the extract (given orally: 100, 200 and 400 mg/kg) was established using the in vivo chemical models (acetic acid-induced abdominal constriction and formalin-induced paw licking test) and thermal (hot plate test) of nociception.
30166109	15	12	theme	animal	2086:2091	arg1	time					2074:2077	stay time	2069:2077	the stay time of the animal in the rota-rod	2065:2107	However, the motor coordination test indicated that in the time of 120 min the extract decreases the stay time of the animal in the rota-rod.
30166109	18	13	dep	CONCLUSION	2426:2435	arg1	suggests					2455:2462	suggests	2455:2462	suggests that Pc-EtOH possesses peripheral and central antinociceptive action, and showed potential in inhibition of release of mediators of the inflammatory process	2455:2619	CONCLUSION The present study suggests that Pc-EtOH possesses peripheral and central antinociceptive action, and showed potential in inhibition of release of mediators of the inflammatory process.
30166109	12	14	theme	writhing	1580:1587	arg1	number					1570:1575	the number	1566:1575	the number of writhing	1566:1587	At all doses tested the Pc-EtOH significantly reduced the number of writhing and decreased the paw licking time in both phases of the formalin test (p < 0.05).
30166109	17	15	theme	oxide	2419:2423	arg1	number					2350:2355	the number	2346:2355	the number of neutrophils	2346:2370	In the peritonitis test, acute pre-treatment with Pc-EtOH inhibited leukocyte migration, with a reduction in the number of neutrophils and concentration of total proteins and nitric oxide.
30166109	17	15	theme	oxide	2419:2423	arg1	concentration					2376:2388	concentration	2376:2388	concentration	2376:2388	In the peritonitis test, acute pre-treatment with Pc-EtOH inhibited leukocyte migration, with a reduction in the number of neutrophils and concentration of total proteins and nitric oxide.
30166109	6	16	theme	chemical	581:588	arg1	composition					590:600	The chemical composition	577:600	METHODS The chemical composition of Pc-EtOH	569:611	METHODS The chemical composition of Pc-EtOH was assessed by high-performance liquid chromatography coupled to diode array detector (HPLC-DAD).
30166109	14	17	theme	peripheral	1823:1832	arg1	pathways					1834:1841	central and peripheral pathways	1811:1841	central and peripheral pathways	1811:1841	The antinociceptive mechanism probably involves central and peripheral pathways, involving the pathway of opioid and muscarinic receptors with influence of potassium channels and the nitric oxide pathway.
30166109	18	18	theme	present	2441:2447	arg1	study					2449:2453	The present study	2437:2453	The present study	2437:2453	CONCLUSION The present study suggests that Pc-EtOH possesses peripheral and central antinociceptive action, and showed potential in inhibition of release of mediators of the inflammatory process.
30166109	13	19	theme	plate	1683:1687	arg1	test					1689:1692	the hot plate test	1675:1692	the hot plate test	1675:1692	In the hot plate test, the extract increased the reaction time, reducing painful behavior.
30166109	9	20	theme	treatment	1214:1222	arg1	interference					1186:1197	the possible interference	1173:1197	the possible interference of the extract treatment in motor performance	1173:1243	The rota-rod test was used to verify the possible interference of the extract treatment in motor performance.
30166109	1	21	theme	inflammatory	110:121	arg1	events					123:128	nociceptive and inflammatory events	94:128	nociceptive and inflammatory events	94:128	(Passifloraceae) reduces nociceptive and inflammatory events in mice.
30166109	3	22	theme	several	273:279	arg1	disorders					328:336	inflammatory disorders	315:336	inflammatory disorders	315:336	is described as a native species from the Caatinga biome, and used by traditional medicine for several pharmacological purposes, such as inflammatory disorders.
30166109	3	22	theme	several	273:279	arg1	purposes					297:304	several pharmacological purposes	273:304	several pharmacological purposes	273:304	is described as a native species from the Caatinga biome, and used by traditional medicine for several pharmacological purposes, such as inflammatory disorders.
30166109	17	23	theme	peritonitis	2244:2254	arg1	test					2256:2259	the peritonitis test	2240:2259	the peritonitis test	2240:2259	In the peritonitis test, acute pre-treatment with Pc-EtOH inhibited leukocyte migration, with a reduction in the number of neutrophils and concentration of total proteins and nitric oxide.
30166109	15	24	dep	time	2074:2077	arg1	stay					2069:2072	stay	2069:2072	stay	2069:2072	However, the motor coordination test indicated that in the time of 120 min the extract decreases the stay time of the animal in the rota-rod.
30166109	15	25	from	animal	2086:2091	arg1	rota-rod					2100:2107	the rota-rod	2096:2107	the rota-rod	2096:2107	However, the motor coordination test indicated that in the time of 120 min the extract decreases the stay time of the animal in the rota-rod.
30166109	6	26	theme	diode	679:683	arg1	HPLC-DAD					701:708	HPLC-DAD	701:708	HPLC-DAD	701:708	METHODS The chemical composition of Pc-EtOH was assessed by high-performance liquid chromatography coupled to diode array detector (HPLC-DAD).
30166109	6	26	theme	diode	679:683	arg1	detector					691:698	diode array detector	679:698	diode array detector (HPLC-DAD)	679:709	METHODS The chemical composition of Pc-EtOH was assessed by high-performance liquid chromatography coupled to diode array detector (HPLC-DAD).
30166109	9	27	theme	motor	1227:1231	arg1	performance					1233:1243	motor performance	1227:1243	motor performance	1227:1243	The rota-rod test was used to verify the possible interference of the extract treatment in motor performance.
30166109	5	28	theme	Passiflora	528:537	arg1	Pc-EtOH					551:557	Pc-EtOH	551:557	Pc-EtOH	551:557	HYPOTHESIS/PURPOSE This paper aims to evaluate the antinociceptive and anti-inflammatory activities of the aerial parts of Passiflora cincinnata (Pc-EtOH) in mice.
30166109	5	28	theme	Passiflora	528:537	arg1	cincinnata					539:548	Passiflora cincinnata	528:548	Passiflora cincinnata (Pc-EtOH)	528:558	HYPOTHESIS/PURPOSE This paper aims to evaluate the antinociceptive and anti-inflammatory activities of the aerial parts of Passiflora cincinnata (Pc-EtOH) in mice.
30166109	3	29	theme	inflammatory	315:326	arg1	disorders					328:336	inflammatory disorders	315:336	inflammatory disorders	315:336	is described as a native species from the Caatinga biome, and used by traditional medicine for several pharmacological purposes, such as inflammatory disorders.
30166109	1	30	dep	reduces	86:92	arg1	Passifloraceae					70:83	Passifloraceae	70:83	Passifloraceae	70:83	(Passifloraceae) reduces nociceptive and inflammatory events in mice.
30166109	0	31	theme	cincinnata	52:61	arg1	Mast					63:66	Passiflora cincinnata Mast	41:66	Passiflora cincinnata Mast	41:66	Ethanolic extract of the aerial parts of Passiflora cincinnata Mast.
30166109	7	32	theme	nociception	959:969	arg1	test					950:953	hot plate test	940:953	hot plate test	940:953	The antinociceptive profile of the extract (given orally: 100, 200 and 400 mg/kg) was established using the in vivo chemical models (acetic acid-induced abdominal constriction and formalin-induced paw licking test) and thermal (hot plate test) of nociception.
30166109	7	32	theme	nociception	959:969	arg1	thermal					931:937	thermal	931:937	thermal	931:937	The antinociceptive profile of the extract (given orally: 100, 200 and 400 mg/kg) was established using the in vivo chemical models (acetic acid-induced abdominal constriction and formalin-induced paw licking test) and thermal (hot plate test) of nociception.
30166109	14	33	theme	antinociceptive	1767:1781	arg1	mechanism					1783:1791	The antinociceptive mechanism	1763:1791	The antinociceptive mechanism	1763:1791	The antinociceptive mechanism probably involves central and peripheral pathways, involving the pathway of opioid and muscarinic receptors with influence of potassium channels and the nitric oxide pathway.
30166109	10	34	theme	anti-inflammatory	1405:1421	arg1	profile					1423:1429	anti-inflammatory profile	1405:1429	anti-inflammatory profile	1405:1429	Paw edema induced by carrageenan or histamine, and leukocyte migration, determination of total protein and nitric oxide to the peritoneal cavity were used for anti-inflammatory profile.
30166109	6	35	theme	array	685:689	arg1	HPLC-DAD					701:708	HPLC-DAD	701:708	HPLC-DAD	701:708	METHODS The chemical composition of Pc-EtOH was assessed by high-performance liquid chromatography coupled to diode array detector (HPLC-DAD).
30166109	6	35	theme	array	685:689	arg1	detector					691:698	diode array detector	679:698	diode array detector (HPLC-DAD)	679:709	METHODS The chemical composition of Pc-EtOH was assessed by high-performance liquid chromatography coupled to diode array detector (HPLC-DAD).
30166109	6	36	theme	liquid	646:651	arg1	chromatography					653:666	high-performance liquid chromatography	629:666	high-performance liquid chromatography coupled to diode array detector (HPLC-DAD)	629:709	METHODS The chemical composition of Pc-EtOH was assessed by high-performance liquid chromatography coupled to diode array detector (HPLC-DAD).
30166109	8	37	theme	channels	1002:1009	arg1	role					976:979	The role	972:979	The role of opioid, potassium channels, TRPV-1, muscarinic, serotoninergic (5-HT3) receptors and the participation of the nitric oxide pathway	972:1113	The role of opioid, potassium channels, TRPV-1, muscarinic, serotoninergic (5-HT3) receptors and the participation of the nitric oxide pathway also was determined.
30166109	12	38	theme	test	1655:1658	arg1	phases					1632:1637	both phases	1627:1637	both phases of the formalin test (p < 0.05)	1627:1669	At all doses tested the Pc-EtOH significantly reduced the number of writhing and decreased the paw licking time in both phases of the formalin test (p < 0.05).
30166109	15	39	theme	motor	1981:1985	arg1	test					2000:2003	the motor coordination test	1977:2003	the motor coordination test	1977:2003	However, the motor coordination test indicated that in the time of 120 min the extract decreases the stay time of the animal in the rota-rod.
30166109	16	40	dep	significantly	2128:2140	arg1	p < 0.05					2143:2150	p < 0.05	2143:2150	p < 0.05	2143:2150	Pc-EtOH inhibited significantly (p < 0.05) the increase of the edema volume after administration of carrageenan and histamine.
30166109	8	41	theme	TRPV-1	1012:1017	arg1	role					976:979	The role	972:979	The role of opioid, potassium channels, TRPV-1, muscarinic, serotoninergic (5-HT3) receptors and the participation of the nitric oxide pathway	972:1113	The role of opioid, potassium channels, TRPV-1, muscarinic, serotoninergic (5-HT3) receptors and the participation of the nitric oxide pathway also was determined.
30166109	7	42	theme	plate	944:948	arg1	test					950:953	hot plate test	940:953	hot plate test	940:953	The antinociceptive profile of the extract (given orally: 100, 200 and 400 mg/kg) was established using the in vivo chemical models (acetic acid-induced abdominal constriction and formalin-induced paw licking test) and thermal (hot plate test) of nociception.
30166109	7	42	theme	plate	944:948	arg1	thermal					931:937	thermal	931:937	thermal	931:937	The antinociceptive profile of the extract (given orally: 100, 200 and 400 mg/kg) was established using the in vivo chemical models (acetic acid-induced abdominal constriction and formalin-induced paw licking test) and thermal (hot plate test) of nociception.
30166109	17	43	from	reduction	2333:2341	arg1	number					2350:2355	the number	2346:2355	the number of neutrophils	2346:2370	In the peritonitis test, acute pre-treatment with Pc-EtOH inhibited leukocyte migration, with a reduction in the number of neutrophils and concentration of total proteins and nitric oxide.
30166109	17	43	from	reduction	2333:2341	arg1	concentration					2376:2388	concentration	2376:2388	concentration	2376:2388	In the peritonitis test, acute pre-treatment with Pc-EtOH inhibited leukocyte migration, with a reduction in the number of neutrophils and concentration of total proteins and nitric oxide.
30166109	18	44	theme	inflammatory	2600:2611	arg1	process					2613:2619	the inflammatory process	2596:2619	the inflammatory process	2596:2619	CONCLUSION The present study suggests that Pc-EtOH possesses peripheral and central antinociceptive action, and showed potential in inhibition of release of mediators of the inflammatory process.
30166109	8	45	theme	muscarinic	1020:1029	arg1	role					976:979	The role	972:979	The role of opioid, potassium channels, TRPV-1, muscarinic, serotoninergic (5-HT3) receptors and the participation of the nitric oxide pathway	972:1113	The role of opioid, potassium channels, TRPV-1, muscarinic, serotoninergic (5-HT3) receptors and the participation of the nitric oxide pathway also was determined.
30166109	18	46	theme	release	2572:2578	arg1	inhibition					2558:2567	inhibition	2558:2567	inhibition of release of mediators of the inflammatory process	2558:2619	CONCLUSION The present study suggests that Pc-EtOH possesses peripheral and central antinociceptive action, and showed potential in inhibition of release of mediators of the inflammatory process.
30166109	11	47	attach	presence	1444:1451	arg1	extract					1474:1480	the extract	1470:1480	the extract	1470:1480	RESULTS The presence of flavonoids in the extract was confirmed using HPLC-DAD.
30166109	11	47	attach	presence	1444:1451	arg2	flavonoids					1456:1465	flavonoids	1456:1465	flavonoids	1456:1465	RESULTS The presence of flavonoids in the extract was confirmed using HPLC-DAD.
30166109	3	48	theme	native	196:201	arg1	species					203:209	a native species	194:209	a native species from the Caatinga biome	194:233	is described as a native species from the Caatinga biome, and used by traditional medicine for several pharmacological purposes, such as inflammatory disorders.
30166109	9	49	used	used	1158:1161	arg2	test					1149:1152	The rota-rod test	1136:1152	The rota-rod test	1136:1152	The rota-rod test was used to verify the possible interference of the extract treatment in motor performance.
30166109	16	50	theme	edema	2173:2177	arg1	volume					2179:2184	the edema volume	2169:2184	the edema volume	2169:2184	Pc-EtOH inhibited significantly (p < 0.05) the increase of the edema volume after administration of carrageenan and histamine.
30166109	0	51	theme	Ethanolic	0:8	arg1	extract					10:16	Ethanolic extract	0:16	Ethanolic extract of the aerial parts of Passiflora cincinnata Mast.	0:67	Ethanolic extract of the aerial parts of Passiflora cincinnata Mast.
30166109	3	52	from	biome	229:233	arg1	species					203:209	a native species	194:209	a native species from the Caatinga biome	194:233	is described as a native species from the Caatinga biome, and used by traditional medicine for several pharmacological purposes, such as inflammatory disorders.
30166109	8	53	theme	serotoninergic	1032:1045	arg1	receptors					1055:1063	serotoninergic (5-HT3) receptors	1032:1063	serotoninergic (5-HT3) receptors	1032:1063	The role of opioid, potassium channels, TRPV-1, muscarinic, serotoninergic (5-HT3) receptors and the participation of the nitric oxide pathway also was determined.
30166109	14	54	theme	nitric	1946:1951	arg1	oxide					1953:1957	nitric oxide	1946:1957	the nitric oxide pathway	1942:1965	The antinociceptive mechanism probably involves central and peripheral pathways, involving the pathway of opioid and muscarinic receptors with influence of potassium channels and the nitric oxide pathway.
30166109	14	55	theme	channels	1929:1936	arg1	influence					1906:1914	influence	1906:1914	influence of potassium channels	1906:1936	The antinociceptive mechanism probably involves central and peripheral pathways, involving the pathway of opioid and muscarinic receptors with influence of potassium channels and the nitric oxide pathway.
30166109	8	56	theme	5-HT3	1048:1052	arg1	receptors					1055:1063	serotoninergic (5-HT3) receptors	1032:1063	serotoninergic (5-HT3) receptors	1032:1063	The role of opioid, potassium channels, TRPV-1, muscarinic, serotoninergic (5-HT3) receptors and the participation of the nitric oxide pathway also was determined.
30166109	7	57	dep	given	756:760	arg1	400 mg/kg					783:791	400 mg/kg	783:791	400 mg/kg	783:791	The antinociceptive profile of the extract (given orally: 100, 200 and 400 mg/kg) was established using the in vivo chemical models (acetic acid-induced abdominal constriction and formalin-induced paw licking test) and thermal (hot plate test) of nociception.
30166109	7	57	dep	given	756:760	arg1	200					775:777	200	775:777	200	775:777	The antinociceptive profile of the extract (given orally: 100, 200 and 400 mg/kg) was established using the in vivo chemical models (acetic acid-induced abdominal constriction and formalin-induced paw licking test) and thermal (hot plate test) of nociception.
30166109	7	57	dep	given	756:760	arg1	100					770:772	100	770:772	100	770:772	The antinociceptive profile of the extract (given orally: 100, 200 and 400 mg/kg) was established using the in vivo chemical models (acetic acid-induced abdominal constriction and formalin-induced paw licking test) and thermal (hot plate test) of nociception.
30166109	1	58	theme	nociceptive	94:104	arg1	events					123:128	nociceptive and inflammatory events	94:128	nociceptive and inflammatory events	94:128	(Passifloraceae) reduces nociceptive and inflammatory events in mice.
30166109	12	59	theme	licking	1611:1617	arg1	time					1619:1622	the paw licking time	1603:1622	the paw licking time	1603:1622	At all doses tested the Pc-EtOH significantly reduced the number of writhing and decreased the paw licking time in both phases of the formalin test (p < 0.05).
30166109	7	60	theme	acid-induced	852:863	arg1	constriction					875:886	acetic acid-induced abdominal constriction	845:886	acetic acid-induced abdominal constriction	845:886	The antinociceptive profile of the extract (given orally: 100, 200 and 400 mg/kg) was established using the in vivo chemical models (acetic acid-induced abdominal constriction and formalin-induced paw licking test) and thermal (hot plate test) of nociception.
30166109	7	60	theme	acid-induced	852:863	arg1	models					837:842	the in vivo chemical models	816:842	the in vivo chemical models (acetic acid-induced abdominal constriction and formalin-induced paw licking test)	816:925	The antinociceptive profile of the extract (given orally: 100, 200 and 400 mg/kg) was established using the in vivo chemical models (acetic acid-induced abdominal constriction and formalin-induced paw licking test) and thermal (hot plate test) of nociception.
30166109	0	61	theme	aerial	25:30	arg1	parts					32:36	the aerial parts	21:36	the aerial parts of Passiflora cincinnata Mast	21:66	Ethanolic extract of the aerial parts of Passiflora cincinnata Mast.
30166109	8	62	theme	receptors	1055:1063	arg1	role					976:979	The role	972:979	The role of opioid, potassium channels, TRPV-1, muscarinic, serotoninergic (5-HT3) receptors and the participation of the nitric oxide pathway	972:1113	The role of opioid, potassium channels, TRPV-1, muscarinic, serotoninergic (5-HT3) receptors and the participation of the nitric oxide pathway also was determined.
30166109	14	63	theme	potassium	1919:1927	arg1	channels					1929:1936	potassium channels	1919:1936	potassium channels	1919:1936	The antinociceptive mechanism probably involves central and peripheral pathways, involving the pathway of opioid and muscarinic receptors with influence of potassium channels and the nitric oxide pathway.
30166109	2	64	theme	cincinnata	161:170	arg1	Mast					172:175	BACKGROUND Passiflora cincinnata Mast	139:175	BACKGROUND Passiflora cincinnata Mast.	139:176	BACKGROUND Passiflora cincinnata Mast.
30166109	10	65	theme	leukocyte	1297:1305	arg1	migration					1307:1315	leukocyte migration	1297:1315	leukocyte migration	1297:1315	Paw edema induced by carrageenan or histamine, and leukocyte migration, determination of total protein and nitric oxide to the peritoneal cavity were used for anti-inflammatory profile.
30166109	17	66	theme	neutrophils	2360:2370	arg1	number					2350:2355	the number	2346:2355	the number of neutrophils	2346:2370	In the peritonitis test, acute pre-treatment with Pc-EtOH inhibited leukocyte migration, with a reduction in the number of neutrophils and concentration of total proteins and nitric oxide.
30166109	17	66	theme	neutrophils	2360:2370	arg1	concentration					2376:2388	concentration	2376:2388	concentration	2376:2388	In the peritonitis test, acute pre-treatment with Pc-EtOH inhibited leukocyte migration, with a reduction in the number of neutrophils and concentration of total proteins and nitric oxide.
30166109	15	67	from	rota-rod	2100:2107	arg1	time					2074:2077	stay time	2069:2077	the stay time of the animal in the rota-rod	2065:2107	However, the motor coordination test indicated that in the time of 120 min the extract decreases the stay time of the animal in the rota-rod.
30166109	7	68	dep	extract	747:753	arg1	given					756:760	given	756:760	given orally	756:767	The antinociceptive profile of the extract (given orally: 100, 200 and 400 mg/kg) was established using the in vivo chemical models (acetic acid-induced abdominal constriction and formalin-induced paw licking test) and thermal (hot plate test) of nociception.
30166109	14	69	theme	receptors	1891:1899	arg1	pathway					1858:1864	the pathway	1854:1864	the pathway of opioid and muscarinic receptors with influence of potassium channels	1854:1936	The antinociceptive mechanism probably involves central and peripheral pathways, involving the pathway of opioid and muscarinic receptors with influence of potassium channels and the nitric oxide pathway.
30166109	14	69	theme	receptors	1891:1899	arg1	pathway					1959:1965	the nitric oxide pathway	1942:1965	the nitric oxide pathway	1942:1965	The antinociceptive mechanism probably involves central and peripheral pathways, involving the pathway of opioid and muscarinic receptors with influence of potassium channels and the nitric oxide pathway.
30166109	7	70	dep	in	820:821	arg1	vivo					823:826	vivo	823:826	vivo	823:826	The antinociceptive profile of the extract (given orally: 100, 200 and 400 mg/kg) was established using the in vivo chemical models (acetic acid-induced abdominal constriction and formalin-induced paw licking test) and thermal (hot plate test) of nociception.
30166109	7	71	theme	licking	913:919	arg1	test					921:924	formalin-induced paw licking test	892:924	formalin-induced paw licking test	892:924	The antinociceptive profile of the extract (given orally: 100, 200 and 400 mg/kg) was established using the in vivo chemical models (acetic acid-induced abdominal constriction and formalin-induced paw licking test) and thermal (hot plate test) of nociception.
30166109	7	71	theme	licking	913:919	arg1	models					837:842	the in vivo chemical models	816:842	the in vivo chemical models (acetic acid-induced abdominal constriction and formalin-induced paw licking test)	816:925	The antinociceptive profile of the extract (given orally: 100, 200 and 400 mg/kg) was established using the in vivo chemical models (acetic acid-induced abdominal constriction and formalin-induced paw licking test) and thermal (hot plate test) of nociception.
30166109	8	72	theme	nitric	1094:1099	arg1	oxide					1101:1105	the nitric oxide	1090:1105	the nitric oxide pathway	1090:1113	The role of opioid, potassium channels, TRPV-1, muscarinic, serotoninergic (5-HT3) receptors and the participation of the nitric oxide pathway also was determined.
30166109	13	73	theme	painful	1745:1751	arg1	behavior					1753:1760	painful behavior	1745:1760	painful behavior	1745:1760	In the hot plate test, the extract increased the reaction time, reducing painful behavior.
30166109	12	74	dep	reduced	1558:1564	arg1	tested					1525:1530	tested	1525:1530	tested	1525:1530	At all doses tested the Pc-EtOH significantly reduced the number of writhing and decreased the paw licking time in both phases of the formalin test (p < 0.05).
30166109	7	75	theme	formalin-induced	892:907	arg1	test					921:924	formalin-induced paw licking test	892:924	formalin-induced paw licking test	892:924	The antinociceptive profile of the extract (given orally: 100, 200 and 400 mg/kg) was established using the in vivo chemical models (acetic acid-induced abdominal constriction and formalin-induced paw licking test) and thermal (hot plate test) of nociception.
30166109	7	75	theme	formalin-induced	892:907	arg1	models					837:842	the in vivo chemical models	816:842	the in vivo chemical models (acetic acid-induced abdominal constriction and formalin-induced paw licking test)	816:925	The antinociceptive profile of the extract (given orally: 100, 200 and 400 mg/kg) was established using the in vivo chemical models (acetic acid-induced abdominal constriction and formalin-induced paw licking test) and thermal (hot plate test) of nociception.
30166109	7	76	theme	extract	747:753	arg1	profile					732:738	The antinociceptive profile	712:738	The antinociceptive profile of the extract (given orally: 100, 200 and 400 mg/kg)	712:792	The antinociceptive profile of the extract (given orally: 100, 200 and 400 mg/kg) was established using the in vivo chemical models (acetic acid-induced abdominal constriction and formalin-induced paw licking test) and thermal (hot plate test) of nociception.
30166109	8	77	theme	pathway	1107:1113	arg1	participation					1073:1085	the participation	1069:1085	the participation of the nitric oxide pathway	1069:1113	The role of opioid, potassium channels, TRPV-1, muscarinic, serotoninergic (5-HT3) receptors and the participation of the nitric oxide pathway also was determined.
30166109	8	77	theme	pathway	1107:1113	arg1	channels					1002:1009	potassium channels	992:1009	potassium channels	992:1009	The role of opioid, potassium channels, TRPV-1, muscarinic, serotoninergic (5-HT3) receptors and the participation of the nitric oxide pathway also was determined.
30166109	8	77	theme	pathway	1107:1113	arg1	TRPV-1					1012:1017	TRPV-1	1012:1017	TRPV-1	1012:1017	The role of opioid, potassium channels, TRPV-1, muscarinic, serotoninergic (5-HT3) receptors and the participation of the nitric oxide pathway also was determined.
30166109	8	77	theme	pathway	1107:1113	arg1	receptors					1055:1063	serotoninergic (5-HT3) receptors	1032:1063	serotoninergic (5-HT3) receptors	1032:1063	The role of opioid, potassium channels, TRPV-1, muscarinic, serotoninergic (5-HT3) receptors and the participation of the nitric oxide pathway also was determined.
30166109	8	77	theme	pathway	1107:1113	arg1	opioid					984:989	opioid	984:989	opioid	984:989	The role of opioid, potassium channels, TRPV-1, muscarinic, serotoninergic (5-HT3) receptors and the participation of the nitric oxide pathway also was determined.
30166109	8	77	theme	pathway	1107:1113	arg1	muscarinic					1020:1029	muscarinic	1020:1029	muscarinic	1020:1029	The role of opioid, potassium channels, TRPV-1, muscarinic, serotoninergic (5-HT3) receptors and the participation of the nitric oxide pathway also was determined.
30166109	3	78	theme	Caatinga	220:227	arg1	biome					229:233	the Caatinga biome	216:233	the Caatinga biome	216:233	is described as a native species from the Caatinga biome, and used by traditional medicine for several pharmacological purposes, such as inflammatory disorders.
30166109	17	79	theme	leukocyte	2305:2313	arg1	migration					2315:2323	leukocyte migration	2305:2323	leukocyte migration	2305:2323	In the peritonitis test, acute pre-treatment with Pc-EtOH inhibited leukocyte migration, with a reduction in the number of neutrophils and concentration of total proteins and nitric oxide.
30166109	18	80	theme	mediators	2583:2591	arg1	release					2572:2578	release	2572:2578	release of mediators of the inflammatory process	2572:2619	CONCLUSION The present study suggests that Pc-EtOH possesses peripheral and central antinociceptive action, and showed potential in inhibition of release of mediators of the inflammatory process.
30166109	10	81	theme	nitric	1353:1358	arg1	oxide					1360:1364	nitric oxide	1353:1364	nitric oxide to the peritoneal cavity	1353:1389	Paw edema induced by carrageenan or histamine, and leukocyte migration, determination of total protein and nitric oxide to the peritoneal cavity were used for anti-inflammatory profile.
30166109	13	82	theme	reaction	1721:1728	arg1	time					1730:1733	the reaction time	1717:1733	the reaction time	1717:1733	In the hot plate test, the extract increased the reaction time, reducing painful behavior.
30166109	2	83	theme	BACKGROUND	139:148	arg1	Mast					172:175	BACKGROUND Passiflora cincinnata Mast	139:175	BACKGROUND Passiflora cincinnata Mast.	139:176	BACKGROUND Passiflora cincinnata Mast.
30166109	10	84	theme	protein	1341:1347	arg1	determination					1318:1330	determination	1318:1330	determination of total protein	1318:1347	Paw edema induced by carrageenan or histamine, and leukocyte migration, determination of total protein and nitric oxide to the peritoneal cavity were used for anti-inflammatory profile.
30166109	10	84	theme	protein	1341:1347	arg1	migration					1307:1315	leukocyte migration	1297:1315	leukocyte migration	1297:1315	Paw edema induced by carrageenan or histamine, and leukocyte migration, determination of total protein and nitric oxide to the peritoneal cavity were used for anti-inflammatory profile.
30166109	10	84	theme	protein	1341:1347	arg1	oxide					1360:1364	nitric oxide	1353:1364	nitric oxide to the peritoneal cavity	1353:1389	Paw edema induced by carrageenan or histamine, and leukocyte migration, determination of total protein and nitric oxide to the peritoneal cavity were used for anti-inflammatory profile.
30166109	5	85	theme	aerial	512:517	arg1	parts					519:523	the aerial parts	508:523	the aerial parts of Passiflora cincinnata (Pc-EtOH)	508:558	HYPOTHESIS/PURPOSE This paper aims to evaluate the antinociceptive and anti-inflammatory activities of the aerial parts of Passiflora cincinnata (Pc-EtOH) in mice.
30166109	14	86	theme	opioid	1869:1874	arg1	receptors					1891:1899	opioid and muscarinic receptors	1869:1899	receptors	1891:1899	The antinociceptive mechanism probably involves central and peripheral pathways, involving the pathway of opioid and muscarinic receptors with influence of potassium channels and the nitric oxide pathway.
30166109	8	87	theme	opioid	984:989	arg1	role					976:979	The role	972:979	The role of opioid, potassium channels, TRPV-1, muscarinic, serotoninergic (5-HT3) receptors and the participation of the nitric oxide pathway	972:1113	The role of opioid, potassium channels, TRPV-1, muscarinic, serotoninergic (5-HT3) receptors and the participation of the nitric oxide pathway also was determined.
30166109	15	88	theme	coordination	1987:1998	arg1	test					2000:2003	the motor coordination test	1977:2003	the motor coordination test	1977:2003	However, the motor coordination test indicated that in the time of 120 min the extract decreases the stay time of the animal in the rota-rod.
30166109	18	89	theme	central	2502:2508	arg1	action					2526:2531	peripheral and central antinociceptive action	2487:2531	peripheral and central antinociceptive action	2487:2531	CONCLUSION The present study suggests that Pc-EtOH possesses peripheral and central antinociceptive action, and showed potential in inhibition of release of mediators of the inflammatory process.
30166109	10	90	theme	peritoneal	1373:1382	arg1	cavity					1384:1389	the peritoneal cavity	1369:1389	the peritoneal cavity	1369:1389	Paw edema induced by carrageenan or histamine, and leukocyte migration, determination of total protein and nitric oxide to the peritoneal cavity were used for anti-inflammatory profile.
30166109	4	91	theme	biological	371:380	arg1	activities					382:391	its biological activities	367:391	its biological activities	367:391	However, studies that prove its biological activities are scarce.
30166109	17	92	theme	proteins	2399:2406	arg1	number					2350:2355	the number	2346:2355	the number of neutrophils	2346:2370	In the peritonitis test, acute pre-treatment with Pc-EtOH inhibited leukocyte migration, with a reduction in the number of neutrophils and concentration of total proteins and nitric oxide.
30166109	17	92	theme	proteins	2399:2406	arg1	concentration					2376:2388	concentration	2376:2388	concentration	2376:2388	In the peritonitis test, acute pre-treatment with Pc-EtOH inhibited leukocyte migration, with a reduction in the number of neutrophils and concentration of total proteins and nitric oxide.
30166109	6	93	theme	Pc-EtOH	605:611	arg1	composition					590:600	The chemical composition	577:600	METHODS The chemical composition of Pc-EtOH	569:611	METHODS The chemical composition of Pc-EtOH was assessed by high-performance liquid chromatography coupled to diode array detector (HPLC-DAD).
30166109	8	94	theme	potassium	992:1000	arg1	channels					1002:1009	potassium channels	992:1009	potassium channels	992:1009	The role of opioid, potassium channels, TRPV-1, muscarinic, serotoninergic (5-HT3) receptors and the participation of the nitric oxide pathway also was determined.
30166109	9	95	theme	possible	1177:1184	arg1	interference					1186:1197	the possible interference	1173:1197	the possible interference of the extract treatment in motor performance	1173:1243	The rota-rod test was used to verify the possible interference of the extract treatment in motor performance.
30166109	5	96	theme	anti-inflammatory	476:492	arg1	activities					494:503	the antinociceptive and anti-inflammatory activities	452:503	the antinociceptive and anti-inflammatory activities of the aerial parts of Passiflora cincinnata (Pc-EtOH) in mice	452:566	HYPOTHESIS/PURPOSE This paper aims to evaluate the antinociceptive and anti-inflammatory activities of the aerial parts of Passiflora cincinnata (Pc-EtOH) in mice.
30166109	6	97	dep	METHODS	569:575	arg1	composition					590:600	The chemical composition	577:600	METHODS The chemical composition of Pc-EtOH	569:611	METHODS The chemical composition of Pc-EtOH was assessed by high-performance liquid chromatography coupled to diode array detector (HPLC-DAD).
30166109	17	98	theme	nitric	2412:2417	arg1	oxide					2419:2423	nitric oxide	2412:2423	nitric oxide	2412:2423	In the peritonitis test, acute pre-treatment with Pc-EtOH inhibited leukocyte migration, with a reduction in the number of neutrophils and concentration of total proteins and nitric oxide.
30166109	7	99	theme	in	820:821	arg1	test					921:924	formalin-induced paw licking test	892:924	formalin-induced paw licking test	892:924	The antinociceptive profile of the extract (given orally: 100, 200 and 400 mg/kg) was established using the in vivo chemical models (acetic acid-induced abdominal constriction and formalin-induced paw licking test) and thermal (hot plate test) of nociception.
30166109	7	99	theme	in	820:821	arg1	constriction					875:886	acetic acid-induced abdominal constriction	845:886	acetic acid-induced abdominal constriction	845:886	The antinociceptive profile of the extract (given orally: 100, 200 and 400 mg/kg) was established using the in vivo chemical models (acetic acid-induced abdominal constriction and formalin-induced paw licking test) and thermal (hot plate test) of nociception.
30166109	7	99	theme	in	820:821	arg1	models					837:842	the in vivo chemical models	816:842	the in vivo chemical models (acetic acid-induced abdominal constriction and formalin-induced paw licking test)	816:925	The antinociceptive profile of the extract (given orally: 100, 200 and 400 mg/kg) was established using the in vivo chemical models (acetic acid-induced abdominal constriction and formalin-induced paw licking test) and thermal (hot plate test) of nociception.
30166109	3	100	theme	traditional	248:258	arg1	medicine					260:267	traditional medicine	248:267	traditional medicine for several pharmacological purposes, such as inflammatory disorders	248:336	is described as a native species from the Caatinga biome, and used by traditional medicine for several pharmacological purposes, such as inflammatory disorders.
30166109	18	101	theme	peripheral	2487:2496	arg1	action					2526:2531	peripheral and central antinociceptive action	2487:2531	peripheral and central antinociceptive action	2487:2531	CONCLUSION The present study suggests that Pc-EtOH possesses peripheral and central antinociceptive action, and showed potential in inhibition of release of mediators of the inflammatory process.
30166109	15	102	from	time	2074:2077	arg1	rota-rod					2100:2107	the rota-rod	2096:2107	the rota-rod	2096:2107	However, the motor coordination test indicated that in the time of 120 min the extract decreases the stay time of the animal in the rota-rod.
30166109	13	103	theme	hot	1679:1681	arg1	test					1689:1692	the hot plate test	1675:1692	the hot plate test	1675:1692	In the hot plate test, the extract increased the reaction time, reducing painful behavior.
30166109	9	104	theme	extract	1206:1212	arg1	treatment					1214:1222	the extract treatment	1202:1222	the extract treatment	1202:1222	The rota-rod test was used to verify the possible interference of the extract treatment in motor performance.
30166109	11	105	theme	flavonoids	1456:1465	arg1	presence					1444:1451	The presence	1440:1451	The presence of flavonoids in the extract	1440:1480	RESULTS The presence of flavonoids in the extract was confirmed using HPLC-DAD.
30166109	3	106	theme	pharmacological	281:295	arg1	disorders					328:336	inflammatory disorders	315:336	inflammatory disorders	315:336	is described as a native species from the Caatinga biome, and used by traditional medicine for several pharmacological purposes, such as inflammatory disorders.
30166109	3	106	theme	pharmacological	281:295	arg1	purposes					297:304	several pharmacological purposes	273:304	several pharmacological purposes	273:304	is described as a native species from the Caatinga biome, and used by traditional medicine for several pharmacological purposes, such as inflammatory disorders.
30166109	7	107	theme	chemical	828:835	arg1	test					921:924	formalin-induced paw licking test	892:924	formalin-induced paw licking test	892:924	The antinociceptive profile of the extract (given orally: 100, 200 and 400 mg/kg) was established using the in vivo chemical models (acetic acid-induced abdominal constriction and formalin-induced paw licking test) and thermal (hot plate test) of nociception.
30166109	7	107	theme	chemical	828:835	arg1	constriction					875:886	acetic acid-induced abdominal constriction	845:886	acetic acid-induced abdominal constriction	845:886	The antinociceptive profile of the extract (given orally: 100, 200 and 400 mg/kg) was established using the in vivo chemical models (acetic acid-induced abdominal constriction and formalin-induced paw licking test) and thermal (hot plate test) of nociception.
30166109	7	107	theme	chemical	828:835	arg1	models					837:842	the in vivo chemical models	816:842	the in vivo chemical models (acetic acid-induced abdominal constriction and formalin-induced paw licking test)	816:925	The antinociceptive profile of the extract (given orally: 100, 200 and 400 mg/kg) was established using the in vivo chemical models (acetic acid-induced abdominal constriction and formalin-induced paw licking test) and thermal (hot plate test) of nociception.
30166109	0	108	theme	Mast	63:66	arg1	parts					32:36	the aerial parts	21:36	the aerial parts of Passiflora cincinnata Mast	21:66	Ethanolic extract of the aerial parts of Passiflora cincinnata Mast.
30166109	5	109	theme	cincinnata	539:548	arg1	parts					519:523	the aerial parts	508:523	the aerial parts of Passiflora cincinnata (Pc-EtOH)	508:558	HYPOTHESIS/PURPOSE This paper aims to evaluate the antinociceptive and anti-inflammatory activities of the aerial parts of Passiflora cincinnata (Pc-EtOH) in mice.
30166109	11	110	dep	RESULTS	1432:1438	arg1	confirmed					1486:1494	confirmed	1486:1494	was confirmed using HPLC-DAD	1482:1509	RESULTS The presence of flavonoids in the extract was confirmed using HPLC-DAD.
30166109	6	111	theme	high-performance	629:644	arg1	chromatography					653:666	high-performance liquid chromatography	629:666	high-performance liquid chromatography coupled to diode array detector (HPLC-DAD)	629:709	METHODS The chemical composition of Pc-EtOH was assessed by high-performance liquid chromatography coupled to diode array detector (HPLC-DAD).
30166109	12	112	theme	formalin	1646:1653	arg1	p < 0.05					1661:1668	p < 0.05	1661:1668	p < 0.05	1661:1668	At all doses tested the Pc-EtOH significantly reduced the number of writhing and decreased the paw licking time in both phases of the formalin test (p < 0.05).
30166109	12	112	theme	formalin	1646:1653	arg1	test					1655:1658	the formalin test	1642:1658	the formalin test (p < 0.05)	1642:1669	At all doses tested the Pc-EtOH significantly reduced the number of writhing and decreased the paw licking time in both phases of the formalin test (p < 0.05).
30166109	10	113	used	used	1396:1399	arg2	migration					1307:1315	leukocyte migration	1297:1315	leukocyte migration	1297:1315	Paw edema induced by carrageenan or histamine, and leukocyte migration, determination of total protein and nitric oxide to the peritoneal cavity were used for anti-inflammatory profile.
30166109	10	113	used	used	1396:1399	arg2	determination					1318:1330	determination	1318:1330	determination of total protein	1318:1347	Paw edema induced by carrageenan or histamine, and leukocyte migration, determination of total protein and nitric oxide to the peritoneal cavity were used for anti-inflammatory profile.
30166109	10	113	used	used	1396:1399	arg2	oxide					1360:1364	nitric oxide	1353:1364	nitric oxide to the peritoneal cavity	1353:1389	Paw edema induced by carrageenan or histamine, and leukocyte migration, determination of total protein and nitric oxide to the peritoneal cavity were used for anti-inflammatory profile.
30166109	18	114	theme	process	2613:2619	arg1	mediators					2583:2591	mediators	2583:2591	mediators of the inflammatory process	2583:2619	CONCLUSION The present study suggests that Pc-EtOH possesses peripheral and central antinociceptive action, and showed potential in inhibition of release of mediators of the inflammatory process.
30166109	18	115	contain	possesses	2477:2485	arg2	action					2526:2531	peripheral and central antinociceptive action	2487:2531	peripheral and central antinociceptive action	2487:2531	CONCLUSION The present study suggests that Pc-EtOH possesses peripheral and central antinociceptive action, and showed potential in inhibition of release of mediators of the inflammatory process.
30166109	18	115	contain	possesses	2477:2485	arg1	Pc-EtOH					2469:2475	Pc-EtOH	2469:2475	Pc-EtOH	2469:2475	CONCLUSION The present study suggests that Pc-EtOH possesses peripheral and central antinociceptive action, and showed potential in inhibition of release of mediators of the inflammatory process.
30166109	10	116	theme	Paw	1246:1248	arg1	edema					1250:1254	Paw edema	1246:1254	Paw edema	1246:1254	Paw edema induced by carrageenan or histamine, and leukocyte migration, determination of total protein and nitric oxide to the peritoneal cavity were used for anti-inflammatory profile.
30166109	14	117	with	receptors	1891:1899	arg1	influence					1906:1914	influence	1906:1914	influence of potassium channels	1906:1936	The antinociceptive mechanism probably involves central and peripheral pathways, involving the pathway of opioid and muscarinic receptors with influence of potassium channels and the nitric oxide pathway.
30166109	7	118	theme	hot	940:942	arg1	test					950:953	hot plate test	940:953	hot plate test	940:953	The antinociceptive profile of the extract (given orally: 100, 200 and 400 mg/kg) was established using the in vivo chemical models (acetic acid-induced abdominal constriction and formalin-induced paw licking test) and thermal (hot plate test) of nociception.
30166109	7	118	theme	hot	940:942	arg1	thermal					931:937	thermal	931:937	thermal	931:937	The antinociceptive profile of the extract (given orally: 100, 200 and 400 mg/kg) was established using the in vivo chemical models (acetic acid-induced abdominal constriction and formalin-induced paw licking test) and thermal (hot plate test) of nociception.
30166109	11	119	from	presence	1444:1451	arg1	extract					1474:1480	the extract	1470:1480	the extract	1470:1480	RESULTS The presence of flavonoids in the extract was confirmed using HPLC-DAD.
30166109	5	120	dep	HYPOTHESIS/PURPOSE	405:422	arg1	aims					435:438	aims	435:438	aims to evaluate the antinociceptive and anti-inflammatory activities of the aerial parts of Passiflora cincinnata (Pc-EtOH) in mice	435:566	HYPOTHESIS/PURPOSE This paper aims to evaluate the antinociceptive and anti-inflammatory activities of the aerial parts of Passiflora cincinnata (Pc-EtOH) in mice.
30166109	5	121	theme	antinociceptive	456:470	arg1	activities					494:503	the antinociceptive and anti-inflammatory activities	452:503	the antinociceptive and anti-inflammatory activities of the aerial parts of Passiflora cincinnata (Pc-EtOH) in mice	452:566	HYPOTHESIS/PURPOSE This paper aims to evaluate the antinociceptive and anti-inflammatory activities of the aerial parts of Passiflora cincinnata (Pc-EtOH) in mice.
30166109	16	122	theme	carrageenan	2210:2220	arg1	administration					2192:2205	administration	2192:2205	administration of carrageenan and histamine	2192:2234	Pc-EtOH inhibited significantly (p < 0.05) the increase of the edema volume after administration of carrageenan and histamine.
30166109	0	123	theme	parts	32:36	arg1	extract					10:16	Ethanolic extract	0:16	Ethanolic extract of the aerial parts of Passiflora cincinnata Mast.	0:67	Ethanolic extract of the aerial parts of Passiflora cincinnata Mast.
30166109	5	124	from	activities	494:503	arg1	mice					563:566	mice	563:566	mice	563:566	HYPOTHESIS/PURPOSE This paper aims to evaluate the antinociceptive and anti-inflammatory activities of the aerial parts of Passiflora cincinnata (Pc-EtOH) in mice.
30166109	12	125	theme	paw	1607:1609	arg1	time					1619:1622	the paw licking time	1603:1622	the paw licking time	1603:1622	At all doses tested the Pc-EtOH significantly reduced the number of writhing and decreased the paw licking time in both phases of the formalin test (p < 0.05).
30166109	7	126	dep	models	837:842	arg1	test					921:924	formalin-induced paw licking test	892:924	formalin-induced paw licking test	892:924	The antinociceptive profile of the extract (given orally: 100, 200 and 400 mg/kg) was established using the in vivo chemical models (acetic acid-induced abdominal constriction and formalin-induced paw licking test) and thermal (hot plate test) of nociception.
30166109	7	126	dep	models	837:842	arg1	constriction					875:886	acetic acid-induced abdominal constriction	845:886	acetic acid-induced abdominal constriction	845:886	The antinociceptive profile of the extract (given orally: 100, 200 and 400 mg/kg) was established using the in vivo chemical models (acetic acid-induced abdominal constriction and formalin-induced paw licking test) and thermal (hot plate test) of nociception.
30166109	7	126	dep	models	837:842	arg1	models					837:842	the in vivo chemical models	816:842	the in vivo chemical models (acetic acid-induced abdominal constriction and formalin-induced paw licking test)	816:925	The antinociceptive profile of the extract (given orally: 100, 200 and 400 mg/kg) was established using the in vivo chemical models (acetic acid-induced abdominal constriction and formalin-induced paw licking test) and thermal (hot plate test) of nociception.
30166109	0	127	theme	Passiflora	41:50	arg1	Mast					63:66	Passiflora cincinnata Mast	41:66	Passiflora cincinnata Mast	41:66	Ethanolic extract of the aerial parts of Passiflora cincinnata Mast.
30166109	8	128	theme	participation	1073:1085	arg1	role					976:979	The role	972:979	The role of opioid, potassium channels, TRPV-1, muscarinic, serotoninergic (5-HT3) receptors and the participation of the nitric oxide pathway	972:1113	The role of opioid, potassium channels, TRPV-1, muscarinic, serotoninergic (5-HT3) receptors and the participation of the nitric oxide pathway also was determined.
30166109	14	129	theme	central	1811:1817	arg1	pathways					1834:1841	central and peripheral pathways	1811:1841	central and peripheral pathways	1811:1841	The antinociceptive mechanism probably involves central and peripheral pathways, involving the pathway of opioid and muscarinic receptors with influence of potassium channels and the nitric oxide pathway.
30166109	15	130	theme	120 min	2035:2041	arg1	time					2027:2030	the time	2023:2030	the time of 120 min	2023:2041	However, the motor coordination test indicated that in the time of 120 min the extract decreases the stay time of the animal in the rota-rod.
30166109	17	131	theme	acute	2262:2266	arg1	pre-treatment					2268:2280	acute pre-treatment	2262:2280	acute pre-treatment with Pc-EtOH	2262:2293	In the peritonitis test, acute pre-treatment with Pc-EtOH inhibited leukocyte migration, with a reduction in the number of neutrophils and concentration of total proteins and nitric oxide.
30166109	10	132	theme	total	1335:1339	arg1	protein					1341:1347	total protein	1335:1347	total protein	1335:1347	Paw edema induced by carrageenan or histamine, and leukocyte migration, determination of total protein and nitric oxide to the peritoneal cavity were used for anti-inflammatory profile.
30166109	7	133	theme	paw	909:911	arg1	test					921:924	formalin-induced paw licking test	892:924	formalin-induced paw licking test	892:924	The antinociceptive profile of the extract (given orally: 100, 200 and 400 mg/kg) was established using the in vivo chemical models (acetic acid-induced abdominal constriction and formalin-induced paw licking test) and thermal (hot plate test) of nociception.
30166109	7	133	theme	paw	909:911	arg1	models					837:842	the in vivo chemical models	816:842	the in vivo chemical models (acetic acid-induced abdominal constriction and formalin-induced paw licking test)	816:925	The antinociceptive profile of the extract (given orally: 100, 200 and 400 mg/kg) was established using the in vivo chemical models (acetic acid-induced abdominal constriction and formalin-induced paw licking test) and thermal (hot plate test) of nociception.
30166109	16	134	theme	volume	2179:2184	arg1	increase					2157:2164	the increase	2153:2164	the increase of the edema volume	2153:2184	Pc-EtOH inhibited significantly (p < 0.05) the increase of the edema volume after administration of carrageenan and histamine.
30166109	8	135	theme	oxide	1101:1105	arg1	pathway					1107:1113	the nitric oxide pathway	1090:1113	the nitric oxide pathway	1090:1113	The role of opioid, potassium channels, TRPV-1, muscarinic, serotoninergic (5-HT3) receptors and the participation of the nitric oxide pathway also was determined.
30166109	14	136	theme	muscarinic	1880:1889	arg1	receptors					1891:1899	opioid and muscarinic receptors	1869:1899	receptors	1891:1899	The antinociceptive mechanism probably involves central and peripheral pathways, involving the pathway of opioid and muscarinic receptors with influence of potassium channels and the nitric oxide pathway.
31075346	7	0	theme	reporter	1483:1490	arg1	expression					1502:1511	reporter transgene expression	1483:1511	reporter transgene expression	1483:1511	Furthermore, we identify a star-PLL composition with 64 poly(˪-lysine) arms and 5 (˪-lysine) subunits per arm as a particularly effective vector, capable of facilitating a 2-fold increase in reporter transgene expression compared to the widely used vector polyethylenimine (PEI), a 44-fold increase compared to a 32 poly(˪-lysine) armed star-PLL and a 130-fold increase compared to its linear analogue, linear poly(˪-lysine) (L-PLL) from a collagen-chondroitin sulfate gene activated scaffold.
31075346	8	1	theme	subcutaneous	1800:1811	arg1	model					1821:1825	an in vivo subcutaneous implant model	1789:1825	an in vivo subcutaneous implant model	1789:1825	In an in vivo subcutaneous implant model, star-PLL-pDNA gene activated scaffolds which were implanted cell-free exhibited extensive infiltration of autologous host cells, nanomedicine retention within the implanted construct and successful host cell transfection at the very early time point of just seven days.
31075346	3	2	theme	scaffold	525:532	arg1	platform					534:541	a versatile, collagen based, gene-activated scaffold platform	481:541	a versatile, collagen based, gene-activated scaffold platform which can transfect autologous host cells in vivo via incorporation of star-shaped poly(˪-lysine) polypeptides (star-PLLs) and a plasmid DNA (pDNA) cargo	481:695	Herein, we describe a versatile, collagen based, gene-activated scaffold platform which can transfect autologous host cells in vivo via incorporation of star-shaped poly(˪-lysine) polypeptides (star-PLLs) and a plasmid DNA (pDNA) cargo.
31075346	7	3	theme	sulfate	1753:1759	arg1	gene					1761:1764	a collagen-chondroitin sulfate gene	1730:1764	a collagen-chondroitin sulfate gene activated scaffold	1730:1783	Furthermore, we identify a star-PLL composition with 64 poly(˪-lysine) arms and 5 (˪-lysine) subunits per arm as a particularly effective vector, capable of facilitating a 2-fold increase in reporter transgene expression compared to the widely used vector polyethylenimine (PEI), a 44-fold increase compared to a 32 poly(˪-lysine) armed star-PLL and a 130-fold increase compared to its linear analogue, linear poly(˪-lysine) (L-PLL) from a collagen-chondroitin sulfate gene activated scaffold.
31075346	6	4	theme	release	1209:1215	arg1	control					1185:1191	enhanced spatiotemporal control	1161:1191	enhanced spatiotemporal control of nanomedicine release from functionalized scaffolds	1161:1245	We demonstrate that the star-PLL structure confers enhanced spatiotemporal control of nanomedicine release from functionalized scaffolds over a 28-day period compared to naked pDNA.
31075346	3	5	theme	host	574:577	arg1	cells					579:583	autologous host cells	563:583	autologous host cells	563:583	Herein, we describe a versatile, collagen based, gene-activated scaffold platform which can transfect autologous host cells in vivo via incorporation of star-shaped poly(˪-lysine) polypeptides (star-PLLs) and a plasmid DNA (pDNA) cargo.
31075346	8	6	dep	in	1792:1793	arg1	vivo					1795:1798	vivo	1795:1798	vivo	1795:1798	In an in vivo subcutaneous implant model, star-PLL-pDNA gene activated scaffolds which were implanted cell-free exhibited extensive infiltration of autologous host cells, nanomedicine retention within the implanted construct and successful host cell transfection at the very early time point of just seven days.
31075346	5	7	dep	prolonged	1034:1042	arg1	non-toxic					1045:1053	non-toxic	1045:1053	non-toxic	1045:1053	In vitro, the functionalization of a range of collagen based scaffolds containing either glycosaminoglycans (chondroitin sulfate or hyaluronic acid) or ceramics (hydroxyapatite or nano-hydroxyapatite) with star-PLL-pDNA nanomedicines facilitated prolonged, non-toxic transgene expression by mesenchymal stem cells (MSCs).
31075346	8	8	theme	star-PLL-pDNA	1828:1840	arg1	scaffolds					1857:1865	star-PLL-pDNA gene activated scaffolds	1828:1865	star-PLL-pDNA gene activated scaffolds which were implanted cell-free	1828:1896	In an in vivo subcutaneous implant model, star-PLL-pDNA gene activated scaffolds which were implanted cell-free exhibited extensive infiltration of autologous host cells, nanomedicine retention within the implanted construct and successful host cell transfection at the very early time point of just seven days.
31075346	1	9	theme	engineering	182:192	arg1	field					166:170	the field	162:170	the field of tissue engineering	162:192	It is increasingly being recognised within the field of tissue engineering that the regenerative capacity of biomaterial scaffolds can be augmented via the incorporation of gene therapeutics.
31075346	6	10	theme	spatiotemporal	1170:1183	arg1	control					1185:1191	enhanced spatiotemporal control	1161:1191	enhanced spatiotemporal control of nanomedicine release from functionalized scaffolds	1161:1245	We demonstrate that the star-PLL structure confers enhanced spatiotemporal control of nanomedicine release from functionalized scaffolds over a 28-day period compared to naked pDNA.
31075346	8	11	theme	activated	1847:1855	arg1	scaffolds					1857:1865	star-PLL-pDNA gene activated scaffolds	1828:1865	star-PLL-pDNA gene activated scaffolds which were implanted cell-free	1828:1896	In an in vivo subcutaneous implant model, star-PLL-pDNA gene activated scaffolds which were implanted cell-free exhibited extensive infiltration of autologous host cells, nanomedicine retention within the implanted construct and successful host cell transfection at the very early time point of just seven days.
31075346	5	12	theme	based	843:847	arg1	scaffolds					849:857	collagen based scaffolds	834:857	collagen based scaffolds containing either glycosaminoglycans (chondroitin sulfate or hyaluronic acid) or ceramics (hydroxyapatite or nano-hydroxyapatite)	834:987	In vitro, the functionalization of a range of collagen based scaffolds containing either glycosaminoglycans (chondroitin sulfate or hyaluronic acid) or ceramics (hydroxyapatite or nano-hydroxyapatite) with star-PLL-pDNA nanomedicines facilitated prolonged, non-toxic transgene expression by mesenchymal stem cells (MSCs).
31075346	5	13	theme	mesenchymal	1079:1089	arg1	MSCs					1103:1106	MSCs	1103:1106	MSCs	1103:1106	In vitro, the functionalization of a range of collagen based scaffolds containing either glycosaminoglycans (chondroitin sulfate or hyaluronic acid) or ceramics (hydroxyapatite or nano-hydroxyapatite) with star-PLL-pDNA nanomedicines facilitated prolonged, non-toxic transgene expression by mesenchymal stem cells (MSCs).
31075346	5	13	theme	mesenchymal	1079:1089	arg1	cells					1096:1100	mesenchymal stem cells	1079:1100	mesenchymal stem cells (MSCs)	1079:1107	In vitro, the functionalization of a range of collagen based scaffolds containing either glycosaminoglycans (chondroitin sulfate or hyaluronic acid) or ceramics (hydroxyapatite or nano-hydroxyapatite) with star-PLL-pDNA nanomedicines facilitated prolonged, non-toxic transgene expression by mesenchymal stem cells (MSCs).
31075346	2	14	theme	delivery	363:370	arg1	vector					372:377	a biocompatible gene delivery vector	342:377	a biocompatible gene delivery vector which is capable of functionalizing scaffolds for tailored nucleic acid delivery	342:458	However, the field still lacks a biocompatible gene delivery vector which is capable of functionalizing scaffolds for tailored nucleic acid delivery.
31075346	2	14	theme	delivery	363:370	arg1	capable					388:394	capable	388:394	capable	388:394	However, the field still lacks a biocompatible gene delivery vector which is capable of functionalizing scaffolds for tailored nucleic acid delivery.
31075346	8	15	theme	early	2061:2065	arg1	point					2072:2076	the very early time point	2052:2076	the very early time point of just seven days	2052:2095	In an in vivo subcutaneous implant model, star-PLL-pDNA gene activated scaffolds which were implanted cell-free exhibited extensive infiltration of autologous host cells, nanomedicine retention within the implanted construct and successful host cell transfection at the very early time point of just seven days.
31075346	9	16	theme	significant	2155:2165	arg1	ability					2167:2173	the significant ability	2151:2173	the significant ability of the star-PLL polymeric structure to transfect autologous host cells in vivo from an implanted biomaterial scaffold	2151:2291	Overall, this article illustrates for the first time the significant ability of the star-PLL polymeric structure to transfect autologous host cells in vivo from an implanted biomaterial scaffold thereby forming a versatile platform with potential in numerous tissue engineering applications.
31075346	3	17	theme	poly	626:629	arg1	star-PLLs					655:663	star-PLLs	655:663	star-PLLs	655:663	Herein, we describe a versatile, collagen based, gene-activated scaffold platform which can transfect autologous host cells in vivo via incorporation of star-shaped poly(˪-lysine) polypeptides (star-PLLs) and a plasmid DNA (pDNA) cargo.
31075346	3	17	theme	poly	626:629	arg1	polypeptides					641:652	star-shaped poly(˪-lysine) polypeptides	614:652	star-shaped poly(˪-lysine) polypeptides (star-PLLs)	614:664	Herein, we describe a versatile, collagen based, gene-activated scaffold platform which can transfect autologous host cells in vivo via incorporation of star-shaped poly(˪-lysine) polypeptides (star-PLLs) and a plasmid DNA (pDNA) cargo.
31075346	1	18	theme	biomaterial	228:238	arg1	scaffolds					240:248	biomaterial scaffolds	228:248	biomaterial scaffolds	228:248	It is increasingly being recognised within the field of tissue engineering that the regenerative capacity of biomaterial scaffolds can be augmented via the incorporation of gene therapeutics.
31075346	6	19	theme	28-day	1254:1259	arg1	period					1261:1266	a 28-day period	1252:1266	a 28-day period compared to naked pDNA	1252:1289	We demonstrate that the star-PLL structure confers enhanced spatiotemporal control of nanomedicine release from functionalized scaffolds over a 28-day period compared to naked pDNA.
31075346	4	20	theme	star-PLL	702:709	arg1	vectors					711:717	Two star-PLL vectors	698:717	Two star-PLL vectors with varying number and length of poly(˪-lysine) arms	698:771	Two star-PLL vectors with varying number and length of poly(˪-lysine) arms were assessed.
31075346	5	21	theme	chondroitin	897:907	arg1	glycosaminoglycans					877:894	glycosaminoglycans	877:894	glycosaminoglycans (chondroitin sulfate or hyaluronic acid)	877:935	In vitro, the functionalization of a range of collagen based scaffolds containing either glycosaminoglycans (chondroitin sulfate or hyaluronic acid) or ceramics (hydroxyapatite or nano-hydroxyapatite) with star-PLL-pDNA nanomedicines facilitated prolonged, non-toxic transgene expression by mesenchymal stem cells (MSCs).
31075346	5	21	theme	chondroitin	897:907	arg1	sulfate					909:915	chondroitin sulfate	897:915	chondroitin sulfate	897:915	In vitro, the functionalization of a range of collagen based scaffolds containing either glycosaminoglycans (chondroitin sulfate or hyaluronic acid) or ceramics (hydroxyapatite or nano-hydroxyapatite) with star-PLL-pDNA nanomedicines facilitated prolonged, non-toxic transgene expression by mesenchymal stem cells (MSCs).
31075346	5	22	dep	ceramics	940:947	arg1	nano-hydroxyapatite					968:986	nano-hydroxyapatite	968:986	nano-hydroxyapatite	968:986	In vitro, the functionalization of a range of collagen based scaffolds containing either glycosaminoglycans (chondroitin sulfate or hyaluronic acid) or ceramics (hydroxyapatite or nano-hydroxyapatite) with star-PLL-pDNA nanomedicines facilitated prolonged, non-toxic transgene expression by mesenchymal stem cells (MSCs).
31075346	5	22	dep	ceramics	940:947	arg1	hydroxyapatite					950:963	hydroxyapatite	950:963	hydroxyapatite	950:963	In vitro, the functionalization of a range of collagen based scaffolds containing either glycosaminoglycans (chondroitin sulfate or hyaluronic acid) or ceramics (hydroxyapatite or nano-hydroxyapatite) with star-PLL-pDNA nanomedicines facilitated prolonged, non-toxic transgene expression by mesenchymal stem cells (MSCs).
31075346	5	22	dep	ceramics	940:947	arg1	ceramics					940:947	ceramics	940:947	ceramics (hydroxyapatite or nano-hydroxyapatite)	940:987	In vitro, the functionalization of a range of collagen based scaffolds containing either glycosaminoglycans (chondroitin sulfate or hyaluronic acid) or ceramics (hydroxyapatite or nano-hydroxyapatite) with star-PLL-pDNA nanomedicines facilitated prolonged, non-toxic transgene expression by mesenchymal stem cells (MSCs).
31075346	9	23	theme	star-PLL	2182:2189	arg1	structure					2201:2209	the star-PLL polymeric structure	2178:2209	the star-PLL polymeric structure	2178:2209	Overall, this article illustrates for the first time the significant ability of the star-PLL polymeric structure to transfect autologous host cells in vivo from an implanted biomaterial scaffold thereby forming a versatile platform with potential in numerous tissue engineering applications.
31075346	3	24	theme	polypeptides	641:652	arg1	cargo					691:695	a plasmid DNA (pDNA) cargo	670:695	a plasmid DNA (pDNA) cargo	670:695	Herein, we describe a versatile, collagen based, gene-activated scaffold platform which can transfect autologous host cells in vivo via incorporation of star-shaped poly(˪-lysine) polypeptides (star-PLLs) and a plasmid DNA (pDNA) cargo.
31075346	3	24	theme	polypeptides	641:652	arg1	incorporation					597:609	incorporation	597:609	incorporation of star-shaped poly(˪-lysine) polypeptides (star-PLLs)	597:664	Herein, we describe a versatile, collagen based, gene-activated scaffold platform which can transfect autologous host cells in vivo via incorporation of star-shaped poly(˪-lysine) polypeptides (star-PLLs) and a plasmid DNA (pDNA) cargo.
31075346	7	25	theme	linear	1695:1700	arg1	L-PLL					1718:1722	L-PLL	1718:1722	L-PLL	1718:1722	Furthermore, we identify a star-PLL composition with 64 poly(˪-lysine) arms and 5 (˪-lysine) subunits per arm as a particularly effective vector, capable of facilitating a 2-fold increase in reporter transgene expression compared to the widely used vector polyethylenimine (PEI), a 44-fold increase compared to a 32 poly(˪-lysine) armed star-PLL and a 130-fold increase compared to its linear analogue, linear poly(˪-lysine) (L-PLL) from a collagen-chondroitin sulfate gene activated scaffold.
31075346	7	25	theme	linear	1695:1700	arg1	poly					1702:1705	linear poly	1695:1705	linear poly(˪-lysine) (L-PLL) from a collagen-chondroitin sulfate gene activated scaffold	1695:1783	Furthermore, we identify a star-PLL composition with 64 poly(˪-lysine) arms and 5 (˪-lysine) subunits per arm as a particularly effective vector, capable of facilitating a 2-fold increase in reporter transgene expression compared to the widely used vector polyethylenimine (PEI), a 44-fold increase compared to a 32 poly(˪-lysine) armed star-PLL and a 130-fold increase compared to its linear analogue, linear poly(˪-lysine) (L-PLL) from a collagen-chondroitin sulfate gene activated scaffold.
31075346	7	25	theme	linear	1695:1700	arg1	increase					1653:1660	a 130-fold increase	1642:1660	a 130-fold increase compared to its linear analogue	1642:1692	Furthermore, we identify a star-PLL composition with 64 poly(˪-lysine) arms and 5 (˪-lysine) subunits per arm as a particularly effective vector, capable of facilitating a 2-fold increase in reporter transgene expression compared to the widely used vector polyethylenimine (PEI), a 44-fold increase compared to a 32 poly(˪-lysine) armed star-PLL and a 130-fold increase compared to its linear analogue, linear poly(˪-lysine) (L-PLL) from a collagen-chondroitin sulfate gene activated scaffold.
31075346	7	25	theme	linear	1695:1700	arg1	˪-lysine					1707:1714	˪-lysine	1707:1714	˪-lysine	1707:1714	Furthermore, we identify a star-PLL composition with 64 poly(˪-lysine) arms and 5 (˪-lysine) subunits per arm as a particularly effective vector, capable of facilitating a 2-fold increase in reporter transgene expression compared to the widely used vector polyethylenimine (PEI), a 44-fold increase compared to a 32 poly(˪-lysine) armed star-PLL and a 130-fold increase compared to its linear analogue, linear poly(˪-lysine) (L-PLL) from a collagen-chondroitin sulfate gene activated scaffold.
31075346	8	26	theme	host	2026:2029	arg1	transfection					2036:2047	successful host cell transfection	2015:2047	successful host cell transfection	2015:2047	In an in vivo subcutaneous implant model, star-PLL-pDNA gene activated scaffolds which were implanted cell-free exhibited extensive infiltration of autologous host cells, nanomedicine retention within the implanted construct and successful host cell transfection at the very early time point of just seven days.
31075346	6	27	theme	functionalized	1222:1235	arg1	scaffolds					1237:1245	functionalized scaffolds	1222:1245	functionalized scaffolds	1222:1245	We demonstrate that the star-PLL structure confers enhanced spatiotemporal control of nanomedicine release from functionalized scaffolds over a 28-day period compared to naked pDNA.
31075346	9	28	theme	implanted	2262:2270	arg1	biomaterial					2272:2282	an implanted biomaterial	2259:2282	an implanted biomaterial scaffold	2259:2291	Overall, this article illustrates for the first time the significant ability of the star-PLL polymeric structure to transfect autologous host cells in vivo from an implanted biomaterial scaffold thereby forming a versatile platform with potential in numerous tissue engineering applications.
31075346	9	29	theme	structure	2201:2209	arg1	ability					2167:2173	the significant ability	2151:2173	the significant ability of the star-PLL polymeric structure to transfect autologous host cells in vivo from an implanted biomaterial scaffold	2151:2291	Overall, this article illustrates for the first time the significant ability of the star-PLL polymeric structure to transfect autologous host cells in vivo from an implanted biomaterial scaffold thereby forming a versatile platform with potential in numerous tissue engineering applications.
31075346	8	30	theme	autologous	1934:1943	arg1	cells					1950:1954	autologous host cells	1934:1954	autologous host cells	1934:1954	In an in vivo subcutaneous implant model, star-PLL-pDNA gene activated scaffolds which were implanted cell-free exhibited extensive infiltration of autologous host cells, nanomedicine retention within the implanted construct and successful host cell transfection at the very early time point of just seven days.
31075346	0	31	theme	collagen	67:74	arg1	scaffolds					76:84	collagen scaffolds	67:84	collagen scaffolds incorporating star-polypeptides	67:116	Transfection of autologous host cells in vivo using gene activated collagen scaffolds incorporating star-polypeptides.
31075346	9	32	theme	tissue	2357:2362	arg1	applications					2376:2387	numerous tissue engineering applications	2348:2387	numerous tissue engineering applications	2348:2387	Overall, this article illustrates for the first time the significant ability of the star-PLL polymeric structure to transfect autologous host cells in vivo from an implanted biomaterial scaffold thereby forming a versatile platform with potential in numerous tissue engineering applications.
31075346	4	33	theme	poly	753:756	arg1	arms					768:771	poly(˪-lysine) arms	753:771	poly(˪-lysine) arms	753:771	Two star-PLL vectors with varying number and length of poly(˪-lysine) arms were assessed.
31075346	6	34	from	scaffolds	1237:1245	arg1	control					1185:1191	enhanced spatiotemporal control	1161:1191	enhanced spatiotemporal control of nanomedicine release from functionalized scaffolds	1161:1245	We demonstrate that the star-PLL structure confers enhanced spatiotemporal control of nanomedicine release from functionalized scaffolds over a 28-day period compared to naked pDNA.
31075346	6	34	from	scaffolds	1237:1245	arg1	release					1209:1215	nanomedicine release	1196:1215	nanomedicine release from functionalized scaffolds	1196:1245	We demonstrate that the star-PLL structure confers enhanced spatiotemporal control of nanomedicine release from functionalized scaffolds over a 28-day period compared to naked pDNA.
31075346	9	35	theme	host	2235:2238	arg1	cells					2240:2244	autologous host cells	2224:2244	autologous host cells	2224:2244	Overall, this article illustrates for the first time the significant ability of the star-PLL polymeric structure to transfect autologous host cells in vivo from an implanted biomaterial scaffold thereby forming a versatile platform with potential in numerous tissue engineering applications.
31075346	8	36	theme	nanomedicine	1957:1968	arg1	retention					1970:1978	nanomedicine retention	1957:1978	nanomedicine retention within the implanted construct	1957:2009	In an in vivo subcutaneous implant model, star-PLL-pDNA gene activated scaffolds which were implanted cell-free exhibited extensive infiltration of autologous host cells, nanomedicine retention within the implanted construct and successful host cell transfection at the very early time point of just seven days.
31075346	9	37	theme	engineering	2364:2374	arg1	applications					2376:2387	numerous tissue engineering applications	2348:2387	numerous tissue engineering applications	2348:2387	Overall, this article illustrates for the first time the significant ability of the star-PLL polymeric structure to transfect autologous host cells in vivo from an implanted biomaterial scaffold thereby forming a versatile platform with potential in numerous tissue engineering applications.
31075346	2	38	theme	nucleic	438:444	arg1	delivery					451:458	tailored nucleic acid delivery	429:458	tailored nucleic acid delivery	429:458	However, the field still lacks a biocompatible gene delivery vector which is capable of functionalizing scaffolds for tailored nucleic acid delivery.
31075346	6	39	from	control	1185:1191	arg1	scaffolds					1237:1245	functionalized scaffolds	1222:1245	functionalized scaffolds	1222:1245	We demonstrate that the star-PLL structure confers enhanced spatiotemporal control of nanomedicine release from functionalized scaffolds over a 28-day period compared to naked pDNA.
31075346	7	40	with	subunits	1385:1392	arg1	arms					1363:1366	64 poly(˪-lysine) arms	1345:1366	64 poly(˪-lysine) arms	1345:1366	Furthermore, we identify a star-PLL composition with 64 poly(˪-lysine) arms and 5 (˪-lysine) subunits per arm as a particularly effective vector, capable of facilitating a 2-fold increase in reporter transgene expression compared to the widely used vector polyethylenimine (PEI), a 44-fold increase compared to a 32 poly(˪-lysine) armed star-PLL and a 130-fold increase compared to its linear analogue, linear poly(˪-lysine) (L-PLL) from a collagen-chondroitin sulfate gene activated scaffold.
31075346	7	41	theme	130-fold	1644:1651	arg1	increase					1653:1660	a 130-fold increase	1642:1660	a 130-fold increase compared to its linear analogue	1642:1692	Furthermore, we identify a star-PLL composition with 64 poly(˪-lysine) arms and 5 (˪-lysine) subunits per arm as a particularly effective vector, capable of facilitating a 2-fold increase in reporter transgene expression compared to the widely used vector polyethylenimine (PEI), a 44-fold increase compared to a 32 poly(˪-lysine) armed star-PLL and a 130-fold increase compared to its linear analogue, linear poly(˪-lysine) (L-PLL) from a collagen-chondroitin sulfate gene activated scaffold.
31075346	7	41	theme	130-fold	1644:1651	arg1	poly					1702:1705	linear poly	1695:1705	linear poly(˪-lysine) (L-PLL) from a collagen-chondroitin sulfate gene activated scaffold	1695:1783	Furthermore, we identify a star-PLL composition with 64 poly(˪-lysine) arms and 5 (˪-lysine) subunits per arm as a particularly effective vector, capable of facilitating a 2-fold increase in reporter transgene expression compared to the widely used vector polyethylenimine (PEI), a 44-fold increase compared to a 32 poly(˪-lysine) armed star-PLL and a 130-fold increase compared to its linear analogue, linear poly(˪-lysine) (L-PLL) from a collagen-chondroitin sulfate gene activated scaffold.
31075346	8	42	theme	implanted	1991:1999	arg1	construct					2001:2009	the implanted construct	1987:2009	the implanted construct	1987:2009	In an in vivo subcutaneous implant model, star-PLL-pDNA gene activated scaffolds which were implanted cell-free exhibited extensive infiltration of autologous host cells, nanomedicine retention within the implanted construct and successful host cell transfection at the very early time point of just seven days.
31075346	1	43	theme	gene	292:295	arg1	therapeutics					297:308	gene therapeutics	292:308	gene therapeutics	292:308	It is increasingly being recognised within the field of tissue engineering that the regenerative capacity of biomaterial scaffolds can be augmented via the incorporation of gene therapeutics.
31075346	7	44	theme	poly	1348:1351	arg1	arms					1363:1366	64 poly(˪-lysine) arms	1345:1366	64 poly(˪-lysine) arms	1345:1366	Furthermore, we identify a star-PLL composition with 64 poly(˪-lysine) arms and 5 (˪-lysine) subunits per arm as a particularly effective vector, capable of facilitating a 2-fold increase in reporter transgene expression compared to the widely used vector polyethylenimine (PEI), a 44-fold increase compared to a 32 poly(˪-lysine) armed star-PLL and a 130-fold increase compared to its linear analogue, linear poly(˪-lysine) (L-PLL) from a collagen-chondroitin sulfate gene activated scaffold.
31075346	2	45	theme	acid	446:449	arg1	delivery					451:458	tailored nucleic acid delivery	429:458	tailored nucleic acid delivery	429:458	However, the field still lacks a biocompatible gene delivery vector which is capable of functionalizing scaffolds for tailored nucleic acid delivery.
31075346	0	46	theme	autologous	16:25	arg1	cells					32:36	autologous host cells	16:36	autologous host cells	16:36	Transfection of autologous host cells in vivo using gene activated collagen scaffolds incorporating star-polypeptides.
31075346	7	47	theme	used	1536:1539	arg1	PEI					1566:1568	PEI	1566:1568	PEI	1566:1568	Furthermore, we identify a star-PLL composition with 64 poly(˪-lysine) arms and 5 (˪-lysine) subunits per arm as a particularly effective vector, capable of facilitating a 2-fold increase in reporter transgene expression compared to the widely used vector polyethylenimine (PEI), a 44-fold increase compared to a 32 poly(˪-lysine) armed star-PLL and a 130-fold increase compared to its linear analogue, linear poly(˪-lysine) (L-PLL) from a collagen-chondroitin sulfate gene activated scaffold.
31075346	7	47	theme	used	1536:1539	arg1	polyethylenimine					1548:1563	the widely used vector polyethylenimine	1525:1563	the widely used vector polyethylenimine (PEI)	1525:1569	Furthermore, we identify a star-PLL composition with 64 poly(˪-lysine) arms and 5 (˪-lysine) subunits per arm as a particularly effective vector, capable of facilitating a 2-fold increase in reporter transgene expression compared to the widely used vector polyethylenimine (PEI), a 44-fold increase compared to a 32 poly(˪-lysine) armed star-PLL and a 130-fold increase compared to its linear analogue, linear poly(˪-lysine) (L-PLL) from a collagen-chondroitin sulfate gene activated scaffold.
31075346	7	47	theme	used	1536:1539	arg1	increase					1582:1589	a 44-fold increase	1572:1589	a 44-fold increase compared to a 32 poly(˪-lysine) armed star-PLL	1572:1636	Furthermore, we identify a star-PLL composition with 64 poly(˪-lysine) arms and 5 (˪-lysine) subunits per arm as a particularly effective vector, capable of facilitating a 2-fold increase in reporter transgene expression compared to the widely used vector polyethylenimine (PEI), a 44-fold increase compared to a 32 poly(˪-lysine) armed star-PLL and a 130-fold increase compared to its linear analogue, linear poly(˪-lysine) (L-PLL) from a collagen-chondroitin sulfate gene activated scaffold.
31075346	7	48	theme	armed	1623:1627	arg1	star-PLL					1629:1636	a 32 poly(˪-lysine) armed star-PLL	1603:1636	a 32 poly(˪-lysine) armed star-PLL	1603:1636	Furthermore, we identify a star-PLL composition with 64 poly(˪-lysine) arms and 5 (˪-lysine) subunits per arm as a particularly effective vector, capable of facilitating a 2-fold increase in reporter transgene expression compared to the widely used vector polyethylenimine (PEI), a 44-fold increase compared to a 32 poly(˪-lysine) armed star-PLL and a 130-fold increase compared to its linear analogue, linear poly(˪-lysine) (L-PLL) from a collagen-chondroitin sulfate gene activated scaffold.
31075346	2	49	theme	tailored	429:436	arg1	delivery					451:458	tailored nucleic acid delivery	429:458	tailored nucleic acid delivery	429:458	However, the field still lacks a biocompatible gene delivery vector which is capable of functionalizing scaffolds for tailored nucleic acid delivery.
31075346	1	50	theme	tissue	175:180	arg1	engineering					182:192	tissue engineering	175:192	tissue engineering	175:192	It is increasingly being recognised within the field of tissue engineering that the regenerative capacity of biomaterial scaffolds can be augmented via the incorporation of gene therapeutics.
31075346	0	51	theme	cells	32:36	arg1	Transfection					0:11	Transfection	0:11	Transfection of autologous host cells in vivo using gene	0:55	Transfection of autologous host cells in vivo using gene activated collagen scaffolds incorporating star-polypeptides.
31075346	7	52	theme	star-PLL	1319:1326	arg1	composition					1328:1338	a star-PLL composition	1317:1338	a star-PLL composition with 64 poly(˪-lysine) arms	1317:1366	Furthermore, we identify a star-PLL composition with 64 poly(˪-lysine) arms and 5 (˪-lysine) subunits per arm as a particularly effective vector, capable of facilitating a 2-fold increase in reporter transgene expression compared to the widely used vector polyethylenimine (PEI), a 44-fold increase compared to a 32 poly(˪-lysine) armed star-PLL and a 130-fold increase compared to its linear analogue, linear poly(˪-lysine) (L-PLL) from a collagen-chondroitin sulfate gene activated scaffold.
31075346	7	52	theme	star-PLL	1319:1326	arg1	vector					1430:1435	a particularly effective vector	1405:1435	a particularly effective vector	1405:1435	Furthermore, we identify a star-PLL composition with 64 poly(˪-lysine) arms and 5 (˪-lysine) subunits per arm as a particularly effective vector, capable of facilitating a 2-fold increase in reporter transgene expression compared to the widely used vector polyethylenimine (PEI), a 44-fold increase compared to a 32 poly(˪-lysine) armed star-PLL and a 130-fold increase compared to its linear analogue, linear poly(˪-lysine) (L-PLL) from a collagen-chondroitin sulfate gene activated scaffold.
31075346	7	52	theme	star-PLL	1319:1326	arg1	subunits					1385:1392	5 (˪-lysine) subunits	1372:1392	5 (˪-lysine) subunits per arm	1372:1400	Furthermore, we identify a star-PLL composition with 64 poly(˪-lysine) arms and 5 (˪-lysine) subunits per arm as a particularly effective vector, capable of facilitating a 2-fold increase in reporter transgene expression compared to the widely used vector polyethylenimine (PEI), a 44-fold increase compared to a 32 poly(˪-lysine) armed star-PLL and a 130-fold increase compared to its linear analogue, linear poly(˪-lysine) (L-PLL) from a collagen-chondroitin sulfate gene activated scaffold.
31075346	2	53	theme	gene	358:361	arg1	vector					372:377	a biocompatible gene delivery vector	342:377	a biocompatible gene delivery vector which is capable of functionalizing scaffolds for tailored nucleic acid delivery	342:458	However, the field still lacks a biocompatible gene delivery vector which is capable of functionalizing scaffolds for tailored nucleic acid delivery.
31075346	2	53	theme	gene	358:361	arg1	capable					388:394	capable	388:394	capable	388:394	However, the field still lacks a biocompatible gene delivery vector which is capable of functionalizing scaffolds for tailored nucleic acid delivery.
31075346	8	54	theme	days	2092:2095	arg1	point					2072:2076	the very early time point	2052:2076	the very early time point of just seven days	2052:2095	In an in vivo subcutaneous implant model, star-PLL-pDNA gene activated scaffolds which were implanted cell-free exhibited extensive infiltration of autologous host cells, nanomedicine retention within the implanted construct and successful host cell transfection at the very early time point of just seven days.
31075346	4	55	theme	arms	768:771	arg1	length					743:748	length	743:748	length	743:748	Two star-PLL vectors with varying number and length of poly(˪-lysine) arms were assessed.
31075346	4	55	theme	arms	768:771	arg1	number					732:737	number	732:737	number	732:737	Two star-PLL vectors with varying number and length of poly(˪-lysine) arms were assessed.
31075346	8	56	theme	successful	2015:2024	arg1	transfection					2036:2047	successful host cell transfection	2015:2047	successful host cell transfection	2015:2047	In an in vivo subcutaneous implant model, star-PLL-pDNA gene activated scaffolds which were implanted cell-free exhibited extensive infiltration of autologous host cells, nanomedicine retention within the implanted construct and successful host cell transfection at the very early time point of just seven days.
31075346	1	57	theme	scaffolds	240:248	arg1	capacity					216:223	the regenerative capacity	199:223	the regenerative capacity of biomaterial scaffolds	199:248	It is increasingly being recognised within the field of tissue engineering that the regenerative capacity of biomaterial scaffolds can be augmented via the incorporation of gene therapeutics.
31075346	7	58	theme	transgene	1492:1500	arg1	expression					1502:1511	reporter transgene expression	1483:1511	reporter transgene expression	1483:1511	Furthermore, we identify a star-PLL composition with 64 poly(˪-lysine) arms and 5 (˪-lysine) subunits per arm as a particularly effective vector, capable of facilitating a 2-fold increase in reporter transgene expression compared to the widely used vector polyethylenimine (PEI), a 44-fold increase compared to a 32 poly(˪-lysine) armed star-PLL and a 130-fold increase compared to its linear analogue, linear poly(˪-lysine) (L-PLL) from a collagen-chondroitin sulfate gene activated scaffold.
31075346	9	59	theme	versatile	2311:2319	arg1	platform					2321:2328	a versatile platform	2309:2328	a versatile platform with potential	2309:2343	Overall, this article illustrates for the first time the significant ability of the star-PLL polymeric structure to transfect autologous host cells in vivo from an implanted biomaterial scaffold thereby forming a versatile platform with potential in numerous tissue engineering applications.
31075346	3	60	theme	plasmid	672:678	arg1	pDNA					685:688	pDNA	685:688	pDNA	685:688	Herein, we describe a versatile, collagen based, gene-activated scaffold platform which can transfect autologous host cells in vivo via incorporation of star-shaped poly(˪-lysine) polypeptides (star-PLLs) and a plasmid DNA (pDNA) cargo.
31075346	3	60	theme	plasmid	672:678	arg1	DNA					680:682	plasmid DNA	672:682	a plasmid DNA (pDNA) cargo	670:695	Herein, we describe a versatile, collagen based, gene-activated scaffold platform which can transfect autologous host cells in vivo via incorporation of star-shaped poly(˪-lysine) polypeptides (star-PLLs) and a plasmid DNA (pDNA) cargo.
31075346	5	61	theme	star-PLL-pDNA	994:1006	arg1	nanomedicines					1008:1020	star-PLL-pDNA nanomedicines	994:1020	star-PLL-pDNA nanomedicines	994:1020	In vitro, the functionalization of a range of collagen based scaffolds containing either glycosaminoglycans (chondroitin sulfate or hyaluronic acid) or ceramics (hydroxyapatite or nano-hydroxyapatite) with star-PLL-pDNA nanomedicines facilitated prolonged, non-toxic transgene expression by mesenchymal stem cells (MSCs).
31075346	8	62	theme	implant	1813:1819	arg1	model					1821:1825	an in vivo subcutaneous implant model	1789:1825	an in vivo subcutaneous implant model	1789:1825	In an in vivo subcutaneous implant model, star-PLL-pDNA gene activated scaffolds which were implanted cell-free exhibited extensive infiltration of autologous host cells, nanomedicine retention within the implanted construct and successful host cell transfection at the very early time point of just seven days.
31075346	7	63	theme	2-fold	1464:1469	arg1	increase					1471:1478	a 2-fold increase	1462:1478	a 2-fold increase in reporter transgene expression compared to the widely used vector polyethylenimine (PEI), a 44-fold increase compared to a 32 poly(˪-lysine) armed star-PLL	1462:1636	Furthermore, we identify a star-PLL composition with 64 poly(˪-lysine) arms and 5 (˪-lysine) subunits per arm as a particularly effective vector, capable of facilitating a 2-fold increase in reporter transgene expression compared to the widely used vector polyethylenimine (PEI), a 44-fold increase compared to a 32 poly(˪-lysine) armed star-PLL and a 130-fold increase compared to its linear analogue, linear poly(˪-lysine) (L-PLL) from a collagen-chondroitin sulfate gene activated scaffold.
31075346	7	64	theme	collagen-chondroitin	1732:1751	arg1	gene					1761:1764	a collagen-chondroitin sulfate gene	1730:1764	a collagen-chondroitin sulfate gene activated scaffold	1730:1783	Furthermore, we identify a star-PLL composition with 64 poly(˪-lysine) arms and 5 (˪-lysine) subunits per arm as a particularly effective vector, capable of facilitating a 2-fold increase in reporter transgene expression compared to the widely used vector polyethylenimine (PEI), a 44-fold increase compared to a 32 poly(˪-lysine) armed star-PLL and a 130-fold increase compared to its linear analogue, linear poly(˪-lysine) (L-PLL) from a collagen-chondroitin sulfate gene activated scaffold.
31075346	3	65	theme	autologous	563:572	arg1	cells					579:583	autologous host cells	563:583	autologous host cells	563:583	Herein, we describe a versatile, collagen based, gene-activated scaffold platform which can transfect autologous host cells in vivo via incorporation of star-shaped poly(˪-lysine) polypeptides (star-PLLs) and a plasmid DNA (pDNA) cargo.
31075346	6	66	theme	nanomedicine	1196:1207	arg1	release					1209:1215	nanomedicine release	1196:1215	nanomedicine release from functionalized scaffolds	1196:1245	We demonstrate that the star-PLL structure confers enhanced spatiotemporal control of nanomedicine release from functionalized scaffolds over a 28-day period compared to naked pDNA.
31075346	8	67	theme	gene	1842:1845	arg1	scaffolds					1857:1865	star-PLL-pDNA gene activated scaffolds	1828:1865	star-PLL-pDNA gene activated scaffolds which were implanted cell-free	1828:1896	In an in vivo subcutaneous implant model, star-PLL-pDNA gene activated scaffolds which were implanted cell-free exhibited extensive infiltration of autologous host cells, nanomedicine retention within the implanted construct and successful host cell transfection at the very early time point of just seven days.
31075346	5	68	theme	range	825:829	arg1	functionalization					802:818	the functionalization	798:818	the functionalization of a range of collagen based scaffolds containing either glycosaminoglycans (chondroitin sulfate or hyaluronic acid) or ceramics (hydroxyapatite or nano-hydroxyapatite) with star-PLL-pDNA nanomedicines	798:1020	In vitro, the functionalization of a range of collagen based scaffolds containing either glycosaminoglycans (chondroitin sulfate or hyaluronic acid) or ceramics (hydroxyapatite or nano-hydroxyapatite) with star-PLL-pDNA nanomedicines facilitated prolonged, non-toxic transgene expression by mesenchymal stem cells (MSCs).
31075346	7	69	theme	capable	1438:1444	arg1	composition					1328:1338	a star-PLL composition	1317:1338	a star-PLL composition with 64 poly(˪-lysine) arms	1317:1366	Furthermore, we identify a star-PLL composition with 64 poly(˪-lysine) arms and 5 (˪-lysine) subunits per arm as a particularly effective vector, capable of facilitating a 2-fold increase in reporter transgene expression compared to the widely used vector polyethylenimine (PEI), a 44-fold increase compared to a 32 poly(˪-lysine) armed star-PLL and a 130-fold increase compared to its linear analogue, linear poly(˪-lysine) (L-PLL) from a collagen-chondroitin sulfate gene activated scaffold.
31075346	7	69	theme	capable	1438:1444	arg1	vector					1430:1435	a particularly effective vector	1405:1435	a particularly effective vector	1405:1435	Furthermore, we identify a star-PLL composition with 64 poly(˪-lysine) arms and 5 (˪-lysine) subunits per arm as a particularly effective vector, capable of facilitating a 2-fold increase in reporter transgene expression compared to the widely used vector polyethylenimine (PEI), a 44-fold increase compared to a 32 poly(˪-lysine) armed star-PLL and a 130-fold increase compared to its linear analogue, linear poly(˪-lysine) (L-PLL) from a collagen-chondroitin sulfate gene activated scaffold.
31075346	7	69	theme	capable	1438:1444	arg1	subunits					1385:1392	5 (˪-lysine) subunits	1372:1392	5 (˪-lysine) subunits per arm	1372:1400	Furthermore, we identify a star-PLL composition with 64 poly(˪-lysine) arms and 5 (˪-lysine) subunits per arm as a particularly effective vector, capable of facilitating a 2-fold increase in reporter transgene expression compared to the widely used vector polyethylenimine (PEI), a 44-fold increase compared to a 32 poly(˪-lysine) armed star-PLL and a 130-fold increase compared to its linear analogue, linear poly(˪-lysine) (L-PLL) from a collagen-chondroitin sulfate gene activated scaffold.
31075346	9	70	with	platform	2321:2328	arg1	potential					2335:2343	potential	2335:2343	potential	2335:2343	Overall, this article illustrates for the first time the significant ability of the star-PLL polymeric structure to transfect autologous host cells in vivo from an implanted biomaterial scaffold thereby forming a versatile platform with potential in numerous tissue engineering applications.
31075346	7	71	theme	effective	1420:1428	arg1	composition					1328:1338	a star-PLL composition	1317:1338	a star-PLL composition with 64 poly(˪-lysine) arms	1317:1366	Furthermore, we identify a star-PLL composition with 64 poly(˪-lysine) arms and 5 (˪-lysine) subunits per arm as a particularly effective vector, capable of facilitating a 2-fold increase in reporter transgene expression compared to the widely used vector polyethylenimine (PEI), a 44-fold increase compared to a 32 poly(˪-lysine) armed star-PLL and a 130-fold increase compared to its linear analogue, linear poly(˪-lysine) (L-PLL) from a collagen-chondroitin sulfate gene activated scaffold.
31075346	7	71	theme	effective	1420:1428	arg1	vector					1430:1435	a particularly effective vector	1405:1435	a particularly effective vector	1405:1435	Furthermore, we identify a star-PLL composition with 64 poly(˪-lysine) arms and 5 (˪-lysine) subunits per arm as a particularly effective vector, capable of facilitating a 2-fold increase in reporter transgene expression compared to the widely used vector polyethylenimine (PEI), a 44-fold increase compared to a 32 poly(˪-lysine) armed star-PLL and a 130-fold increase compared to its linear analogue, linear poly(˪-lysine) (L-PLL) from a collagen-chondroitin sulfate gene activated scaffold.
31075346	7	71	theme	effective	1420:1428	arg1	subunits					1385:1392	5 (˪-lysine) subunits	1372:1392	5 (˪-lysine) subunits per arm	1372:1400	Furthermore, we identify a star-PLL composition with 64 poly(˪-lysine) arms and 5 (˪-lysine) subunits per arm as a particularly effective vector, capable of facilitating a 2-fold increase in reporter transgene expression compared to the widely used vector polyethylenimine (PEI), a 44-fold increase compared to a 32 poly(˪-lysine) armed star-PLL and a 130-fold increase compared to its linear analogue, linear poly(˪-lysine) (L-PLL) from a collagen-chondroitin sulfate gene activated scaffold.
31075346	8	72	theme	cells	1950:1954	arg1	retention					1970:1978	nanomedicine retention	1957:1978	nanomedicine retention within the implanted construct	1957:2009	In an in vivo subcutaneous implant model, star-PLL-pDNA gene activated scaffolds which were implanted cell-free exhibited extensive infiltration of autologous host cells, nanomedicine retention within the implanted construct and successful host cell transfection at the very early time point of just seven days.
31075346	8	72	theme	cells	1950:1954	arg1	transfection					2036:2047	successful host cell transfection	2015:2047	successful host cell transfection	2015:2047	In an in vivo subcutaneous implant model, star-PLL-pDNA gene activated scaffolds which were implanted cell-free exhibited extensive infiltration of autologous host cells, nanomedicine retention within the implanted construct and successful host cell transfection at the very early time point of just seven days.
31075346	8	72	theme	cells	1950:1954	arg1	infiltration					1918:1929	extensive infiltration	1908:1929	extensive infiltration of autologous host cells	1908:1954	In an in vivo subcutaneous implant model, star-PLL-pDNA gene activated scaffolds which were implanted cell-free exhibited extensive infiltration of autologous host cells, nanomedicine retention within the implanted construct and successful host cell transfection at the very early time point of just seven days.
31075346	6	73	theme	naked	1280:1284	arg1	pDNA					1286:1289	naked pDNA	1280:1289	naked pDNA	1280:1289	We demonstrate that the star-PLL structure confers enhanced spatiotemporal control of nanomedicine release from functionalized scaffolds over a 28-day period compared to naked pDNA.
31075346	5	74	theme	collagen	834:841	arg1	scaffolds					849:857	collagen based scaffolds	834:857	collagen based scaffolds containing either glycosaminoglycans (chondroitin sulfate or hyaluronic acid) or ceramics (hydroxyapatite or nano-hydroxyapatite)	834:987	In vitro, the functionalization of a range of collagen based scaffolds containing either glycosaminoglycans (chondroitin sulfate or hyaluronic acid) or ceramics (hydroxyapatite or nano-hydroxyapatite) with star-PLL-pDNA nanomedicines facilitated prolonged, non-toxic transgene expression by mesenchymal stem cells (MSCs).
31075346	7	75	theme	˪-lysine	1613:1620	arg1	star-PLL					1629:1636	a 32 poly(˪-lysine) armed star-PLL	1603:1636	a 32 poly(˪-lysine) armed star-PLL	1603:1636	Furthermore, we identify a star-PLL composition with 64 poly(˪-lysine) arms and 5 (˪-lysine) subunits per arm as a particularly effective vector, capable of facilitating a 2-fold increase in reporter transgene expression compared to the widely used vector polyethylenimine (PEI), a 44-fold increase compared to a 32 poly(˪-lysine) armed star-PLL and a 130-fold increase compared to its linear analogue, linear poly(˪-lysine) (L-PLL) from a collagen-chondroitin sulfate gene activated scaffold.
31075346	6	76	theme	enhanced	1161:1168	arg1	control					1185:1191	enhanced spatiotemporal control	1161:1191	enhanced spatiotemporal control of nanomedicine release from functionalized scaffolds	1161:1245	We demonstrate that the star-PLL structure confers enhanced spatiotemporal control of nanomedicine release from functionalized scaffolds over a 28-day period compared to naked pDNA.
31075346	9	77	theme	first	2140:2144	arg1	time					2146:2149	the first time	2136:2149	the first time	2136:2149	Overall, this article illustrates for the first time the significant ability of the star-PLL polymeric structure to transfect autologous host cells in vivo from an implanted biomaterial scaffold thereby forming a versatile platform with potential in numerous tissue engineering applications.
31075346	1	78	theme	regenerative	203:214	arg1	capacity					216:223	the regenerative capacity	199:223	the regenerative capacity of biomaterial scaffolds	199:248	It is increasingly being recognised within the field of tissue engineering that the regenerative capacity of biomaterial scaffolds can be augmented via the incorporation of gene therapeutics.
31075346	7	79	theme	poly	1608:1611	arg1	star-PLL					1629:1636	a 32 poly(˪-lysine) armed star-PLL	1603:1636	a 32 poly(˪-lysine) armed star-PLL	1603:1636	Furthermore, we identify a star-PLL composition with 64 poly(˪-lysine) arms and 5 (˪-lysine) subunits per arm as a particularly effective vector, capable of facilitating a 2-fold increase in reporter transgene expression compared to the widely used vector polyethylenimine (PEI), a 44-fold increase compared to a 32 poly(˪-lysine) armed star-PLL and a 130-fold increase compared to its linear analogue, linear poly(˪-lysine) (L-PLL) from a collagen-chondroitin sulfate gene activated scaffold.
31075346	7	80	with	composition	1328:1338	arg1	arms					1363:1366	64 poly(˪-lysine) arms	1345:1366	64 poly(˪-lysine) arms	1345:1366	Furthermore, we identify a star-PLL composition with 64 poly(˪-lysine) arms and 5 (˪-lysine) subunits per arm as a particularly effective vector, capable of facilitating a 2-fold increase in reporter transgene expression compared to the widely used vector polyethylenimine (PEI), a 44-fold increase compared to a 32 poly(˪-lysine) armed star-PLL and a 130-fold increase compared to its linear analogue, linear poly(˪-lysine) (L-PLL) from a collagen-chondroitin sulfate gene activated scaffold.
31075346	5	81	theme	stem	1091:1094	arg1	MSCs					1103:1106	MSCs	1103:1106	MSCs	1103:1106	In vitro, the functionalization of a range of collagen based scaffolds containing either glycosaminoglycans (chondroitin sulfate or hyaluronic acid) or ceramics (hydroxyapatite or nano-hydroxyapatite) with star-PLL-pDNA nanomedicines facilitated prolonged, non-toxic transgene expression by mesenchymal stem cells (MSCs).
31075346	5	81	theme	stem	1091:1094	arg1	cells					1096:1100	mesenchymal stem cells	1079:1100	mesenchymal stem cells (MSCs)	1079:1107	In vitro, the functionalization of a range of collagen based scaffolds containing either glycosaminoglycans (chondroitin sulfate or hyaluronic acid) or ceramics (hydroxyapatite or nano-hydroxyapatite) with star-PLL-pDNA nanomedicines facilitated prolonged, non-toxic transgene expression by mesenchymal stem cells (MSCs).
31075346	3	82	theme	star-shaped	614:624	arg1	˪-lysine					631:638	˪-lysine	631:638	˪-lysine	631:638	Herein, we describe a versatile, collagen based, gene-activated scaffold platform which can transfect autologous host cells in vivo via incorporation of star-shaped poly(˪-lysine) polypeptides (star-PLLs) and a plasmid DNA (pDNA) cargo.
31075346	3	82	theme	star-shaped	614:624	arg1	poly					626:629	star-shaped poly	614:629	star-shaped poly(˪-lysine) polypeptides (star-PLLs)	614:664	Herein, we describe a versatile, collagen based, gene-activated scaffold platform which can transfect autologous host cells in vivo via incorporation of star-shaped poly(˪-lysine) polypeptides (star-PLLs) and a plasmid DNA (pDNA) cargo.
31075346	7	83	from	increase	1471:1478	arg1	expression					1502:1511	reporter transgene expression	1483:1511	reporter transgene expression	1483:1511	Furthermore, we identify a star-PLL composition with 64 poly(˪-lysine) arms and 5 (˪-lysine) subunits per arm as a particularly effective vector, capable of facilitating a 2-fold increase in reporter transgene expression compared to the widely used vector polyethylenimine (PEI), a 44-fold increase compared to a 32 poly(˪-lysine) armed star-PLL and a 130-fold increase compared to its linear analogue, linear poly(˪-lysine) (L-PLL) from a collagen-chondroitin sulfate gene activated scaffold.
31075346	9	84	dep	illustrates	2120:2130	arg1	forming					2301:2307	forming	2301:2307	forming a versatile platform with potential in numerous tissue engineering applications	2301:2387	Overall, this article illustrates for the first time the significant ability of the star-PLL polymeric structure to transfect autologous host cells in vivo from an implanted biomaterial scaffold thereby forming a versatile platform with potential in numerous tissue engineering applications.
31075346	8	85	theme	extensive	1908:1916	arg1	infiltration					1918:1929	extensive infiltration	1908:1929	extensive infiltration of autologous host cells	1908:1954	In an in vivo subcutaneous implant model, star-PLL-pDNA gene activated scaffolds which were implanted cell-free exhibited extensive infiltration of autologous host cells, nanomedicine retention within the implanted construct and successful host cell transfection at the very early time point of just seven days.
31075346	5	86	theme	transgene	1055:1063	arg1	expression					1065:1074	prolonged, non-toxic transgene expression	1034:1074	prolonged, non-toxic transgene expression by mesenchymal stem cells (MSCs)	1034:1107	In vitro, the functionalization of a range of collagen based scaffolds containing either glycosaminoglycans (chondroitin sulfate or hyaluronic acid) or ceramics (hydroxyapatite or nano-hydroxyapatite) with star-PLL-pDNA nanomedicines facilitated prolonged, non-toxic transgene expression by mesenchymal stem cells (MSCs).
31075346	7	87	theme	44-fold	1574:1580	arg1	polyethylenimine					1548:1563	the widely used vector polyethylenimine	1525:1563	the widely used vector polyethylenimine (PEI)	1525:1569	Furthermore, we identify a star-PLL composition with 64 poly(˪-lysine) arms and 5 (˪-lysine) subunits per arm as a particularly effective vector, capable of facilitating a 2-fold increase in reporter transgene expression compared to the widely used vector polyethylenimine (PEI), a 44-fold increase compared to a 32 poly(˪-lysine) armed star-PLL and a 130-fold increase compared to its linear analogue, linear poly(˪-lysine) (L-PLL) from a collagen-chondroitin sulfate gene activated scaffold.
31075346	7	87	theme	44-fold	1574:1580	arg1	increase					1582:1589	a 44-fold increase	1572:1589	a 44-fold increase compared to a 32 poly(˪-lysine) armed star-PLL	1572:1636	Furthermore, we identify a star-PLL composition with 64 poly(˪-lysine) arms and 5 (˪-lysine) subunits per arm as a particularly effective vector, capable of facilitating a 2-fold increase in reporter transgene expression compared to the widely used vector polyethylenimine (PEI), a 44-fold increase compared to a 32 poly(˪-lysine) armed star-PLL and a 130-fold increase compared to its linear analogue, linear poly(˪-lysine) (L-PLL) from a collagen-chondroitin sulfate gene activated scaffold.
31075346	7	88	from	increase	1653:1660	arg1	expression					1502:1511	reporter transgene expression	1483:1511	reporter transgene expression	1483:1511	Furthermore, we identify a star-PLL composition with 64 poly(˪-lysine) arms and 5 (˪-lysine) subunits per arm as a particularly effective vector, capable of facilitating a 2-fold increase in reporter transgene expression compared to the widely used vector polyethylenimine (PEI), a 44-fold increase compared to a 32 poly(˪-lysine) armed star-PLL and a 130-fold increase compared to its linear analogue, linear poly(˪-lysine) (L-PLL) from a collagen-chondroitin sulfate gene activated scaffold.
31075346	9	89	theme	polymeric	2191:2199	arg1	structure					2201:2209	the star-PLL polymeric structure	2178:2209	the star-PLL polymeric structure	2178:2209	Overall, this article illustrates for the first time the significant ability of the star-PLL polymeric structure to transfect autologous host cells in vivo from an implanted biomaterial scaffold thereby forming a versatile platform with potential in numerous tissue engineering applications.
31075346	8	90	theme	host	1945:1948	arg1	cells					1950:1954	autologous host cells	1934:1954	autologous host cells	1934:1954	In an in vivo subcutaneous implant model, star-PLL-pDNA gene activated scaffolds which were implanted cell-free exhibited extensive infiltration of autologous host cells, nanomedicine retention within the implanted construct and successful host cell transfection at the very early time point of just seven days.
31075346	5	91	theme	scaffolds	849:857	arg1	range					825:829	a range	823:829	a range of collagen based scaffolds containing either glycosaminoglycans (chondroitin sulfate or hyaluronic acid) or ceramics (hydroxyapatite or nano-hydroxyapatite)	823:987	In vitro, the functionalization of a range of collagen based scaffolds containing either glycosaminoglycans (chondroitin sulfate or hyaluronic acid) or ceramics (hydroxyapatite or nano-hydroxyapatite) with star-PLL-pDNA nanomedicines facilitated prolonged, non-toxic transgene expression by mesenchymal stem cells (MSCs).
31075346	5	92	contain	containing	859:868	arg2	glycosaminoglycans					877:894	glycosaminoglycans	877:894	glycosaminoglycans (chondroitin sulfate or hyaluronic acid)	877:935	In vitro, the functionalization of a range of collagen based scaffolds containing either glycosaminoglycans (chondroitin sulfate or hyaluronic acid) or ceramics (hydroxyapatite or nano-hydroxyapatite) with star-PLL-pDNA nanomedicines facilitated prolonged, non-toxic transgene expression by mesenchymal stem cells (MSCs).
31075346	5	92	contain	containing	859:868	arg2	acid					931:934	hyaluronic acid	920:934	hyaluronic acid	920:934	In vitro, the functionalization of a range of collagen based scaffolds containing either glycosaminoglycans (chondroitin sulfate or hyaluronic acid) or ceramics (hydroxyapatite or nano-hydroxyapatite) with star-PLL-pDNA nanomedicines facilitated prolonged, non-toxic transgene expression by mesenchymal stem cells (MSCs).
31075346	5	92	contain	containing	859:868	arg1	scaffolds					849:857	collagen based scaffolds	834:857	collagen based scaffolds containing either glycosaminoglycans (chondroitin sulfate or hyaluronic acid) or ceramics (hydroxyapatite or nano-hydroxyapatite)	834:987	In vitro, the functionalization of a range of collagen based scaffolds containing either glycosaminoglycans (chondroitin sulfate or hyaluronic acid) or ceramics (hydroxyapatite or nano-hydroxyapatite) with star-PLL-pDNA nanomedicines facilitated prolonged, non-toxic transgene expression by mesenchymal stem cells (MSCs).
31075346	5	92	contain	containing	859:868	arg2	nano-hydroxyapatite					968:986	nano-hydroxyapatite	968:986	nano-hydroxyapatite	968:986	In vitro, the functionalization of a range of collagen based scaffolds containing either glycosaminoglycans (chondroitin sulfate or hyaluronic acid) or ceramics (hydroxyapatite or nano-hydroxyapatite) with star-PLL-pDNA nanomedicines facilitated prolonged, non-toxic transgene expression by mesenchymal stem cells (MSCs).
31075346	5	92	contain	containing	859:868	arg2	sulfate					909:915	chondroitin sulfate	897:915	chondroitin sulfate	897:915	In vitro, the functionalization of a range of collagen based scaffolds containing either glycosaminoglycans (chondroitin sulfate or hyaluronic acid) or ceramics (hydroxyapatite or nano-hydroxyapatite) with star-PLL-pDNA nanomedicines facilitated prolonged, non-toxic transgene expression by mesenchymal stem cells (MSCs).
31075346	5	92	contain	containing	859:868	arg2	ceramics					940:947	ceramics	940:947	ceramics (hydroxyapatite or nano-hydroxyapatite)	940:987	In vitro, the functionalization of a range of collagen based scaffolds containing either glycosaminoglycans (chondroitin sulfate or hyaluronic acid) or ceramics (hydroxyapatite or nano-hydroxyapatite) with star-PLL-pDNA nanomedicines facilitated prolonged, non-toxic transgene expression by mesenchymal stem cells (MSCs).
31075346	5	92	contain	containing	859:868	arg2	hydroxyapatite					950:963	hydroxyapatite	950:963	hydroxyapatite	950:963	In vitro, the functionalization of a range of collagen based scaffolds containing either glycosaminoglycans (chondroitin sulfate or hyaluronic acid) or ceramics (hydroxyapatite or nano-hydroxyapatite) with star-PLL-pDNA nanomedicines facilitated prolonged, non-toxic transgene expression by mesenchymal stem cells (MSCs).
31075346	9	93	theme	autologous	2224:2233	arg1	cells					2240:2244	autologous host cells	2224:2244	autologous host cells	2224:2244	Overall, this article illustrates for the first time the significant ability of the star-PLL polymeric structure to transfect autologous host cells in vivo from an implanted biomaterial scaffold thereby forming a versatile platform with potential in numerous tissue engineering applications.
31075346	7	94	theme	linear	1678:1683	arg1	analogue					1685:1692	its linear analogue	1674:1692	its linear analogue	1674:1692	Furthermore, we identify a star-PLL composition with 64 poly(˪-lysine) arms and 5 (˪-lysine) subunits per arm as a particularly effective vector, capable of facilitating a 2-fold increase in reporter transgene expression compared to the widely used vector polyethylenimine (PEI), a 44-fold increase compared to a 32 poly(˪-lysine) armed star-PLL and a 130-fold increase compared to its linear analogue, linear poly(˪-lysine) (L-PLL) from a collagen-chondroitin sulfate gene activated scaffold.
31075346	7	95	from	gene	1761:1764	arg1	L-PLL					1718:1722	L-PLL	1718:1722	L-PLL	1718:1722	Furthermore, we identify a star-PLL composition with 64 poly(˪-lysine) arms and 5 (˪-lysine) subunits per arm as a particularly effective vector, capable of facilitating a 2-fold increase in reporter transgene expression compared to the widely used vector polyethylenimine (PEI), a 44-fold increase compared to a 32 poly(˪-lysine) armed star-PLL and a 130-fold increase compared to its linear analogue, linear poly(˪-lysine) (L-PLL) from a collagen-chondroitin sulfate gene activated scaffold.
31075346	7	95	from	gene	1761:1764	arg1	poly					1702:1705	linear poly	1695:1705	linear poly(˪-lysine) (L-PLL) from a collagen-chondroitin sulfate gene activated scaffold	1695:1783	Furthermore, we identify a star-PLL composition with 64 poly(˪-lysine) arms and 5 (˪-lysine) subunits per arm as a particularly effective vector, capable of facilitating a 2-fold increase in reporter transgene expression compared to the widely used vector polyethylenimine (PEI), a 44-fold increase compared to a 32 poly(˪-lysine) armed star-PLL and a 130-fold increase compared to its linear analogue, linear poly(˪-lysine) (L-PLL) from a collagen-chondroitin sulfate gene activated scaffold.
31075346	7	95	from	gene	1761:1764	arg1	increase					1653:1660	a 130-fold increase	1642:1660	a 130-fold increase compared to its linear analogue	1642:1692	Furthermore, we identify a star-PLL composition with 64 poly(˪-lysine) arms and 5 (˪-lysine) subunits per arm as a particularly effective vector, capable of facilitating a 2-fold increase in reporter transgene expression compared to the widely used vector polyethylenimine (PEI), a 44-fold increase compared to a 32 poly(˪-lysine) armed star-PLL and a 130-fold increase compared to its linear analogue, linear poly(˪-lysine) (L-PLL) from a collagen-chondroitin sulfate gene activated scaffold.
31075346	7	95	from	gene	1761:1764	arg1	˪-lysine					1707:1714	˪-lysine	1707:1714	˪-lysine	1707:1714	Furthermore, we identify a star-PLL composition with 64 poly(˪-lysine) arms and 5 (˪-lysine) subunits per arm as a particularly effective vector, capable of facilitating a 2-fold increase in reporter transgene expression compared to the widely used vector polyethylenimine (PEI), a 44-fold increase compared to a 32 poly(˪-lysine) armed star-PLL and a 130-fold increase compared to its linear analogue, linear poly(˪-lysine) (L-PLL) from a collagen-chondroitin sulfate gene activated scaffold.
31075346	5	96	theme	hyaluronic	920:929	arg1	glycosaminoglycans					877:894	glycosaminoglycans	877:894	glycosaminoglycans (chondroitin sulfate or hyaluronic acid)	877:935	In vitro, the functionalization of a range of collagen based scaffolds containing either glycosaminoglycans (chondroitin sulfate or hyaluronic acid) or ceramics (hydroxyapatite or nano-hydroxyapatite) with star-PLL-pDNA nanomedicines facilitated prolonged, non-toxic transgene expression by mesenchymal stem cells (MSCs).
31075346	5	96	theme	hyaluronic	920:929	arg1	acid					931:934	hyaluronic acid	920:934	hyaluronic acid	920:934	In vitro, the functionalization of a range of collagen based scaffolds containing either glycosaminoglycans (chondroitin sulfate or hyaluronic acid) or ceramics (hydroxyapatite or nano-hydroxyapatite) with star-PLL-pDNA nanomedicines facilitated prolonged, non-toxic transgene expression by mesenchymal stem cells (MSCs).
31075346	8	97	theme	time	2067:2070	arg1	point					2072:2076	the very early time point	2052:2076	the very early time point of just seven days	2052:2095	In an in vivo subcutaneous implant model, star-PLL-pDNA gene activated scaffolds which were implanted cell-free exhibited extensive infiltration of autologous host cells, nanomedicine retention within the implanted construct and successful host cell transfection at the very early time point of just seven days.
31075346	5	98	dep	glycosaminoglycans	877:894	arg1	glycosaminoglycans					877:894	glycosaminoglycans	877:894	glycosaminoglycans (chondroitin sulfate or hyaluronic acid)	877:935	In vitro, the functionalization of a range of collagen based scaffolds containing either glycosaminoglycans (chondroitin sulfate or hyaluronic acid) or ceramics (hydroxyapatite or nano-hydroxyapatite) with star-PLL-pDNA nanomedicines facilitated prolonged, non-toxic transgene expression by mesenchymal stem cells (MSCs).
31075346	5	98	dep	glycosaminoglycans	877:894	arg1	sulfate					909:915	chondroitin sulfate	897:915	chondroitin sulfate	897:915	In vitro, the functionalization of a range of collagen based scaffolds containing either glycosaminoglycans (chondroitin sulfate or hyaluronic acid) or ceramics (hydroxyapatite or nano-hydroxyapatite) with star-PLL-pDNA nanomedicines facilitated prolonged, non-toxic transgene expression by mesenchymal stem cells (MSCs).
31075346	5	98	dep	glycosaminoglycans	877:894	arg1	acid					931:934	hyaluronic acid	920:934	hyaluronic acid	920:934	In vitro, the functionalization of a range of collagen based scaffolds containing either glycosaminoglycans (chondroitin sulfate or hyaluronic acid) or ceramics (hydroxyapatite or nano-hydroxyapatite) with star-PLL-pDNA nanomedicines facilitated prolonged, non-toxic transgene expression by mesenchymal stem cells (MSCs).
31075346	0	99	theme	host	27:30	arg1	cells					32:36	autologous host cells	16:36	autologous host cells	16:36	Transfection of autologous host cells in vivo using gene activated collagen scaffolds incorporating star-polypeptides.
31075346	1	100	theme	therapeutics	297:308	arg1	incorporation					275:287	the incorporation	271:287	the incorporation of gene therapeutics	271:308	It is increasingly being recognised within the field of tissue engineering that the regenerative capacity of biomaterial scaffolds can be augmented via the incorporation of gene therapeutics.
31075346	8	101	theme	in	1792:1793	arg1	model					1821:1825	an in vivo subcutaneous implant model	1789:1825	an in vivo subcutaneous implant model	1789:1825	In an in vivo subcutaneous implant model, star-PLL-pDNA gene activated scaffolds which were implanted cell-free exhibited extensive infiltration of autologous host cells, nanomedicine retention within the implanted construct and successful host cell transfection at the very early time point of just seven days.
31075346	3	102	theme	versatile	483:491	arg1	platform					534:541	a versatile, collagen based, gene-activated scaffold platform	481:541	a versatile, collagen based, gene-activated scaffold platform which can transfect autologous host cells in vivo via incorporation of star-shaped poly(˪-lysine) polypeptides (star-PLLs) and a plasmid DNA (pDNA) cargo	481:695	Herein, we describe a versatile, collagen based, gene-activated scaffold platform which can transfect autologous host cells in vivo via incorporation of star-shaped poly(˪-lysine) polypeptides (star-PLLs) and a plasmid DNA (pDNA) cargo.
31075346	8	103	theme	cell	2031:2034	arg1	transfection					2036:2047	successful host cell transfection	2015:2047	successful host cell transfection	2015:2047	In an in vivo subcutaneous implant model, star-PLL-pDNA gene activated scaffolds which were implanted cell-free exhibited extensive infiltration of autologous host cells, nanomedicine retention within the implanted construct and successful host cell transfection at the very early time point of just seven days.
31075346	7	104	theme	vector	1541:1546	arg1	PEI					1566:1568	PEI	1566:1568	PEI	1566:1568	Furthermore, we identify a star-PLL composition with 64 poly(˪-lysine) arms and 5 (˪-lysine) subunits per arm as a particularly effective vector, capable of facilitating a 2-fold increase in reporter transgene expression compared to the widely used vector polyethylenimine (PEI), a 44-fold increase compared to a 32 poly(˪-lysine) armed star-PLL and a 130-fold increase compared to its linear analogue, linear poly(˪-lysine) (L-PLL) from a collagen-chondroitin sulfate gene activated scaffold.
31075346	7	104	theme	vector	1541:1546	arg1	polyethylenimine					1548:1563	the widely used vector polyethylenimine	1525:1563	the widely used vector polyethylenimine (PEI)	1525:1569	Furthermore, we identify a star-PLL composition with 64 poly(˪-lysine) arms and 5 (˪-lysine) subunits per arm as a particularly effective vector, capable of facilitating a 2-fold increase in reporter transgene expression compared to the widely used vector polyethylenimine (PEI), a 44-fold increase compared to a 32 poly(˪-lysine) armed star-PLL and a 130-fold increase compared to its linear analogue, linear poly(˪-lysine) (L-PLL) from a collagen-chondroitin sulfate gene activated scaffold.
31075346	7	104	theme	vector	1541:1546	arg1	increase					1582:1589	a 44-fold increase	1572:1589	a 44-fold increase compared to a 32 poly(˪-lysine) armed star-PLL	1572:1636	Furthermore, we identify a star-PLL composition with 64 poly(˪-lysine) arms and 5 (˪-lysine) subunits per arm as a particularly effective vector, capable of facilitating a 2-fold increase in reporter transgene expression compared to the widely used vector polyethylenimine (PEI), a 44-fold increase compared to a 32 poly(˪-lysine) armed star-PLL and a 130-fold increase compared to its linear analogue, linear poly(˪-lysine) (L-PLL) from a collagen-chondroitin sulfate gene activated scaffold.
31075346	5	105	with	functionalization	802:818	arg1	nanomedicines					1008:1020	star-PLL-pDNA nanomedicines	994:1020	star-PLL-pDNA nanomedicines	994:1020	In vitro, the functionalization of a range of collagen based scaffolds containing either glycosaminoglycans (chondroitin sulfate or hyaluronic acid) or ceramics (hydroxyapatite or nano-hydroxyapatite) with star-PLL-pDNA nanomedicines facilitated prolonged, non-toxic transgene expression by mesenchymal stem cells (MSCs).
31075346	5	106	theme	prolonged	1034:1042	arg1	expression					1065:1074	prolonged, non-toxic transgene expression	1034:1074	prolonged, non-toxic transgene expression by mesenchymal stem cells (MSCs)	1034:1107	In vitro, the functionalization of a range of collagen based scaffolds containing either glycosaminoglycans (chondroitin sulfate or hyaluronic acid) or ceramics (hydroxyapatite or nano-hydroxyapatite) with star-PLL-pDNA nanomedicines facilitated prolonged, non-toxic transgene expression by mesenchymal stem cells (MSCs).
31075346	6	107	theme	star-PLL	1134:1141	arg1	structure					1143:1151	the star-PLL structure	1130:1151	the star-PLL structure	1130:1151	We demonstrate that the star-PLL structure confers enhanced spatiotemporal control of nanomedicine release from functionalized scaffolds over a 28-day period compared to naked pDNA.
31075346	3	108	theme	DNA	680:682	arg1	cargo					691:695	a plasmid DNA (pDNA) cargo	670:695	a plasmid DNA (pDNA) cargo	670:695	Herein, we describe a versatile, collagen based, gene-activated scaffold platform which can transfect autologous host cells in vivo via incorporation of star-shaped poly(˪-lysine) polypeptides (star-PLLs) and a plasmid DNA (pDNA) cargo.
31075346	2	109	theme	biocompatible	344:356	arg1	vector					372:377	a biocompatible gene delivery vector	342:377	a biocompatible gene delivery vector which is capable of functionalizing scaffolds for tailored nucleic acid delivery	342:458	However, the field still lacks a biocompatible gene delivery vector which is capable of functionalizing scaffolds for tailored nucleic acid delivery.
31075346	2	109	theme	biocompatible	344:356	arg1	capable					388:394	capable	388:394	capable	388:394	However, the field still lacks a biocompatible gene delivery vector which is capable of functionalizing scaffolds for tailored nucleic acid delivery.
31075346	3	110	dep	versatile	483:491	arg1	based					503:507	based	503:507	based	503:507	Herein, we describe a versatile, collagen based, gene-activated scaffold platform which can transfect autologous host cells in vivo via incorporation of star-shaped poly(˪-lysine) polypeptides (star-PLLs) and a plasmid DNA (pDNA) cargo.
31075346	3	110	dep	versatile	483:491	arg1	gene-activated					510:523	gene-activated	510:523	gene-activated	510:523	Herein, we describe a versatile, collagen based, gene-activated scaffold platform which can transfect autologous host cells in vivo via incorporation of star-shaped poly(˪-lysine) polypeptides (star-PLLs) and a plasmid DNA (pDNA) cargo.
31075346	9	111	theme	biomaterial	2272:2282	arg1	scaffold					2284:2291	an implanted biomaterial scaffold	2259:2291	an implanted biomaterial scaffold	2259:2291	Overall, this article illustrates for the first time the significant ability of the star-PLL polymeric structure to transfect autologous host cells in vivo from an implanted biomaterial scaffold thereby forming a versatile platform with potential in numerous tissue engineering applications.
31075346	9	112	theme	numerous	2348:2355	arg1	applications					2376:2387	numerous tissue engineering applications	2348:2387	numerous tissue engineering applications	2348:2387	Overall, this article illustrates for the first time the significant ability of the star-PLL polymeric structure to transfect autologous host cells in vivo from an implanted biomaterial scaffold thereby forming a versatile platform with potential in numerous tissue engineering applications.
31745651	0	0	theme	acid	102:105	arg1	sensing					65:71	electrochemical sensing	49:71	electrochemical sensing of 2,4-dichlorophenoxyacetic acid	49:105	An imprinted polymeric matrix containing DNA for electrochemical sensing of 2,4-dichlorophenoxyacetic acid.
31745651	8	1	dep	pM	1059:1060	arg1	10					1056:1057	10	1056:1057	10	1056:1057	The current, typically measured at around 0.5 V, increases linearly in the 0.01 to 10 pM 2,4-D concentration range, and the detection limit is 4.0 fM.
31745651	6	2	theme	DNA	674:676	arg1	interaction					659:669	The interaction	655:669	The interaction of DNA with 2,4-D	655:687	The interaction of DNA with 2,4-D leads to its adsorption on the electrode, and this increases the sensitivity and selectivity of the method.
31745651	11	3	theme	sensor	1408:1413	arg1	fabrication					1374:1384	the fabrication	1370:1384	the fabrication of an electrochemical sensor for determination of 2,4-dichlorophenoxyacetic acid (2,4-D)	1370:1473	Graphical abstractSchematic representation of the fabrication of an electrochemical sensor for determination of 2,4-dichlorophenoxyacetic acid (2,4-D).
31745651	12	4	mod	modified	1505:1512	arg3	chitosan					1519:1526	chitosan	1519:1526	chitosan	1519:1526	Initially, the electrode was modified with chitosan and MWCNTs and then a composite was formed on it consisting of ortho-phenylenediamine (oPD), DNA and 2,4-D.
31745651	12	4	mod	modified	1505:1512	arg3	MWCNTs					1532:1537	MWCNTs	1532:1537	MWCNTs	1532:1537	Initially, the electrode was modified with chitosan and MWCNTs and then a composite was formed on it consisting of ortho-phenylenediamine (oPD), DNA and 2,4-D.
31745651	12	4	mod	modified	1505:1512	arg1	electrode					1491:1499	the electrode	1487:1499	the electrode	1487:1499	Initially, the electrode was modified with chitosan and MWCNTs and then a composite was formed on it consisting of ortho-phenylenediamine (oPD), DNA and 2,4-D.
31745651	0	5	theme	2,4-dichlorophenoxyacetic	76:100	arg1	acid					102:105	2,4-dichlorophenoxyacetic acid	76:105	2,4-dichlorophenoxyacetic acid	76:105	An imprinted polymeric matrix containing DNA for electrochemical sensing of 2,4-dichlorophenoxyacetic acid.
31745651	8	6	dep	10	1056:1057	arg1	to					1053:1054	to	1053:1054	to	1053:1054	The current, typically measured at around 0.5 V, increases linearly in the 0.01 to 10 pM 2,4-D concentration range, and the detection limit is 4.0 fM.
31745651	1	7	theme	herbicide	180:188	arg1	2,4-D					222:226	2,4-D	222:226	2,4-D	222:226	The authors describe an electrochemical method for the determination of herbicide 2,4-dichlorophenoxyacetic acid (2,4-D).
31745651	1	7	theme	herbicide	180:188	arg1	acid					216:219	herbicide 2,4-dichlorophenoxyacetic acid	180:219	herbicide 2,4-dichlorophenoxyacetic acid (2,4-D)	180:227	The authors describe an electrochemical method for the determination of herbicide 2,4-dichlorophenoxyacetic acid (2,4-D).
31745651	4	8	theme	imprinted	485:493	arg1	MIP					495:497	The imprinted MIP	481:497	The imprinted MIP	481:497	The imprinted MIP was placed on a pencil graphite electrode (PGE) modified with chitosan and multiwalled carbon nanotubes (MWCNTs).
31745651	11	9	theme	2,4-dichlorophenoxyacetic	1436:1460	arg1	2,4-D					1468:1472	2,4-D	1468:1472	2,4-D	1468:1472	Graphical abstractSchematic representation of the fabrication of an electrochemical sensor for determination of 2,4-dichlorophenoxyacetic acid (2,4-D).
31745651	11	9	theme	2,4-dichlorophenoxyacetic	1436:1460	arg1	acid					1462:1465	2,4-dichlorophenoxyacetic acid	1436:1465	2,4-dichlorophenoxyacetic acid (2,4-D)	1436:1473	Graphical abstractSchematic representation of the fabrication of an electrochemical sensor for determination of 2,4-dichlorophenoxyacetic acid (2,4-D).
31745651	10	10	theme	soil	1253:1256	arg1	samples					1258:1264	spiked environmental water and soil samples	1222:1264	samples	1258:1264	The modified electrode was applied to quantify 2,4-D in spiked environmental water and soil samples and gave absolute recoveries varying from 91.5 to 109.0%.
31745651	10	11	theme	modified	1170:1177	arg1	electrode					1179:1187	The modified electrode	1166:1187	The modified electrode	1166:1187	The modified electrode was applied to quantify 2,4-D in spiked environmental water and soil samples and gave absolute recoveries varying from 91.5 to 109.0%.
31745651	11	12	theme	acid	1462:1465	arg1	determination					1419:1431	determination	1419:1431	determination of 2,4-dichlorophenoxyacetic acid (2,4-D)	1419:1473	Graphical abstractSchematic representation of the fabrication of an electrochemical sensor for determination of 2,4-dichlorophenoxyacetic acid (2,4-D).
31745651	1	13	theme	2,4-dichlorophenoxyacetic	190:214	arg1	2,4-D					222:226	2,4-D	222:226	2,4-D	222:226	The authors describe an electrochemical method for the determination of herbicide 2,4-dichlorophenoxyacetic acid (2,4-D).
31745651	1	13	theme	2,4-dichlorophenoxyacetic	190:214	arg1	acid					216:219	herbicide 2,4-dichlorophenoxyacetic acid	180:219	herbicide 2,4-dichlorophenoxyacetic acid (2,4-D)	180:227	The authors describe an electrochemical method for the determination of herbicide 2,4-dichlorophenoxyacetic acid (2,4-D).
31745651	3	14	theme	modified	340:347	arg1	electrode					349:357	The modified electrode	336:357	The modified electrode	336:357	The modified electrode was prepared by electropolymerization of ortho-phenylenediamine (oPD) in the presence of DNA and of 2,4-D (the template).
31745651	3	15	from	electropolymerization	375:395	arg1	presence					436:443	the presence	432:443	the presence of DNA	432:450	The modified electrode was prepared by electropolymerization of ortho-phenylenediamine (oPD) in the presence of DNA and of 2,4-D (the template).
31745651	2	16	theme	polymer	280:286	arg1	use					249:251	the use	245:251	the use of a molecularly imprinted polymer (MIP) and of dsDNA as a bio-specific substance	245:333	It is based on the use of a molecularly imprinted polymer (MIP) and of dsDNA as a bio-specific substance.
31745651	1	17	theme	acid	216:219	arg1	determination					163:175	the determination	159:175	the determination of herbicide 2,4-dichlorophenoxyacetic acid (2,4-D)	159:227	The authors describe an electrochemical method for the determination of herbicide 2,4-dichlorophenoxyacetic acid (2,4-D).
31745651	0	18	theme	imprinted	3:11	arg1	matrix					23:28	An imprinted polymeric matrix	0:28	An imprinted polymeric matrix	0:28	An imprinted polymeric matrix containing DNA for electrochemical sensing of 2,4-dichlorophenoxyacetic acid.
31745651	10	19	dep	109.0	1316:1320	arg1	to					1313:1314	to	1313:1314	to	1313:1314	The modified electrode was applied to quantify 2,4-D in spiked environmental water and soil samples and gave absolute recoveries varying from 91.5 to 109.0%.
31745651	7	20	theme	iron	869:872	arg1	oxidation					856:864	oxidation	856:864	oxidation of iron(II) acting as an electrochemical redox probe	856:917	After rebinding 2,4-D, the decrease in the peak current of oxidation of iron(II) acting as an electrochemical redox probe was measured by differential pulse voltammetry (DPV).
31745651	10	21	from	2,4-D	1213:1217	arg1	samples					1258:1264	spiked environmental water and soil samples	1222:1264	samples	1258:1264	The modified electrode was applied to quantify 2,4-D in spiked environmental water and soil samples and gave absolute recoveries varying from 91.5 to 109.0%.
31745651	10	21	from	2,4-D	1213:1217	arg1	water					1243:1247	spiked environmental water and soil samples	1222:1264	water	1243:1247	The modified electrode was applied to quantify 2,4-D in spiked environmental water and soil samples and gave absolute recoveries varying from 91.5 to 109.0%.
31745651	8	22	theme	2,4-D	1062:1066	arg1	range					1082:1086	the 0.01 to 10 pM 2,4-D concentration range	1044:1086	the 0.01 to 10 pM 2,4-D concentration range	1044:1086	The current, typically measured at around 0.5 V, increases linearly in the 0.01 to 10 pM 2,4-D concentration range, and the detection limit is 4.0 fM.
31745651	4	23	theme	graphite	522:529	arg1	PGE					542:544	PGE	542:544	PGE	542:544	The imprinted MIP was placed on a pencil graphite electrode (PGE) modified with chitosan and multiwalled carbon nanotubes (MWCNTs).
31745651	4	23	theme	graphite	522:529	arg1	electrode					531:539	a pencil graphite electrode	513:539	a pencil graphite electrode (PGE) modified with chitosan and multiwalled carbon nanotubes (MWCNTs)	513:610	The imprinted MIP was placed on a pencil graphite electrode (PGE) modified with chitosan and multiwalled carbon nanotubes (MWCNTs).
31745651	10	24	theme	absolute	1275:1282	arg1	recoveries					1284:1293	absolute recoveries	1275:1293	absolute recoveries varying from 91.5 to 109.0%	1275:1321	The modified electrode was applied to quantify 2,4-D in spiked environmental water and soil samples and gave absolute recoveries varying from 91.5 to 109.0%.
31745651	2	25	theme	dsDNA	301:305	arg1	use					249:251	the use	245:251	the use of a molecularly imprinted polymer (MIP) and of dsDNA as a bio-specific substance	245:333	It is based on the use of a molecularly imprinted polymer (MIP) and of dsDNA as a bio-specific substance.
31745651	11	26	theme	Graphical	1324:1332	arg1	representation					1352:1365	Graphical abstractSchematic representation	1324:1365	Graphical abstractSchematic representation of the fabrication of an electrochemical sensor for determination of 2,4-dichlorophenoxyacetic acid (2,4-D).	1324:1474	Graphical abstractSchematic representation of the fabrication of an electrochemical sensor for determination of 2,4-dichlorophenoxyacetic acid (2,4-D).
31745651	4	27	theme	pencil	515:520	arg1	PGE					542:544	PGE	542:544	PGE	542:544	The imprinted MIP was placed on a pencil graphite electrode (PGE) modified with chitosan and multiwalled carbon nanotubes (MWCNTs).
31745651	4	27	theme	pencil	515:520	arg1	electrode					531:539	a pencil graphite electrode	513:539	a pencil graphite electrode (PGE) modified with chitosan and multiwalled carbon nanotubes (MWCNTs)	513:610	The imprinted MIP was placed on a pencil graphite electrode (PGE) modified with chitosan and multiwalled carbon nanotubes (MWCNTs).
31745651	7	28	theme	oxidation	856:864	arg1	current					845:851	the peak current	836:851	the peak current of oxidation of iron(II) acting as an electrochemical redox probe	836:917	After rebinding 2,4-D, the decrease in the peak current of oxidation of iron(II) acting as an electrochemical redox probe was measured by differential pulse voltammetry (DPV).
31745651	11	29	theme	abstractSchematic	1334:1350	arg1	representation					1352:1365	Graphical abstractSchematic representation	1324:1365	Graphical abstractSchematic representation of the fabrication of an electrochemical sensor for determination of 2,4-dichlorophenoxyacetic acid (2,4-D).	1324:1474	Graphical abstractSchematic representation of the fabrication of an electrochemical sensor for determination of 2,4-dichlorophenoxyacetic acid (2,4-D).
31745651	7	30	from	decrease	824:831	arg1	current					845:851	the peak current	836:851	the peak current of oxidation of iron(II) acting as an electrochemical redox probe	836:917	After rebinding 2,4-D, the decrease in the peak current of oxidation of iron(II) acting as an electrochemical redox probe was measured by differential pulse voltammetry (DPV).
31745651	0	31	theme	polymeric	13:21	arg1	matrix					23:28	An imprinted polymeric matrix	0:28	An imprinted polymeric matrix	0:28	An imprinted polymeric matrix containing DNA for electrochemical sensing of 2,4-dichlorophenoxyacetic acid.
31745651	6	32	dep	sensitivity	754:764	arg1	the					750:752	the	750:752	the	750:752	The interaction of DNA with 2,4-D leads to its adsorption on the electrode, and this increases the sensitivity and selectivity of the method.
31745651	3	33	theme	2,4-D	459:463	arg1	electropolymerization					375:395	electropolymerization	375:395	electropolymerization of ortho-phenylenediamine (oPD) in the presence of DNA and of 2,4-D (the template)	375:478	The modified electrode was prepared by electropolymerization of ortho-phenylenediamine (oPD) in the presence of DNA and of 2,4-D (the template).
31745651	3	34	theme	DNA	448:450	arg1	presence					436:443	the presence	432:443	the presence of DNA	432:450	The modified electrode was prepared by electropolymerization of ortho-phenylenediamine (oPD) in the presence of DNA and of 2,4-D (the template).
31745651	6	35	with	interaction	659:669	arg1	2,4-D					683:687	2,4-D	683:687	2,4-D	683:687	The interaction of DNA with 2,4-D leads to its adsorption on the electrode, and this increases the sensitivity and selectivity of the method.
31745651	3	36	theme	ortho-phenylenediamine	400:421	arg1	electropolymerization					375:395	electropolymerization	375:395	electropolymerization of ortho-phenylenediamine (oPD) in the presence of DNA and of 2,4-D (the template)	375:478	The modified electrode was prepared by electropolymerization of ortho-phenylenediamine (oPD) in the presence of DNA and of 2,4-D (the template).
31745651	6	37	theme	method	789:794	arg1	selectivity					770:780	selectivity	770:780	selectivity	770:780	The interaction of DNA with 2,4-D leads to its adsorption on the electrode, and this increases the sensitivity and selectivity of the method.
31745651	6	37	theme	method	789:794	arg1	sensitivity					754:764	sensitivity	754:764	sensitivity	754:764	The interaction of DNA with 2,4-D leads to its adsorption on the electrode, and this increases the sensitivity and selectivity of the method.
31745651	7	38	theme	differential	935:946	arg1	voltammetry					954:964	differential pulse voltammetry	935:964	differential pulse voltammetry (DPV)	935:970	After rebinding 2,4-D, the decrease in the peak current of oxidation of iron(II) acting as an electrochemical redox probe was measured by differential pulse voltammetry (DPV).
31745651	7	38	theme	differential	935:946	arg1	DPV					967:969	DPV	967:969	DPV	967:969	After rebinding 2,4-D, the decrease in the peak current of oxidation of iron(II) acting as an electrochemical redox probe was measured by differential pulse voltammetry (DPV).
31745651	8	39	theme	detection	1097:1105	arg1	fM					1120:1121	4.0 fM	1116:1121	4.0 fM	1116:1121	The current, typically measured at around 0.5 V, increases linearly in the 0.01 to 10 pM 2,4-D concentration range, and the detection limit is 4.0 fM.
31745651	8	39	theme	detection	1097:1105	arg1	limit					1107:1111	the detection limit	1093:1111	the detection limit	1093:1111	The current, typically measured at around 0.5 V, increases linearly in the 0.01 to 10 pM 2,4-D concentration range, and the detection limit is 4.0 fM.
31745651	8	40	dep	range	1082:1086	arg1	pM					1059:1060	pM	1059:1060	pM	1059:1060	The current, typically measured at around 0.5 V, increases linearly in the 0.01 to 10 pM 2,4-D concentration range, and the detection limit is 4.0 fM.
31745651	1	41	theme	electrochemical	132:146	arg1	method					148:153	an electrochemical method	129:153	an electrochemical method for the determination of herbicide 2,4-dichlorophenoxyacetic acid (2,4-D)	129:227	The authors describe an electrochemical method for the determination of herbicide 2,4-dichlorophenoxyacetic acid (2,4-D).
31745651	7	42	theme	peak	840:843	arg1	current					845:851	the peak current	836:851	the peak current of oxidation of iron(II) acting as an electrochemical redox probe	836:917	After rebinding 2,4-D, the decrease in the peak current of oxidation of iron(II) acting as an electrochemical redox probe was measured by differential pulse voltammetry (DPV).
31745651	5	43	theme	0.4 M	643:647	arg1	NaOH					649:652	0.4 M NaOH	643:652	0.4 M NaOH	643:652	The template was removed with 0.4 M NaOH.
31745651	2	44	theme	imprinted	270:278	arg1	MIP					289:291	MIP	289:291	MIP	289:291	It is based on the use of a molecularly imprinted polymer (MIP) and of dsDNA as a bio-specific substance.
31745651	2	44	theme	imprinted	270:278	arg1	polymer					280:286	a molecularly imprinted polymer	256:286	a molecularly imprinted polymer (MIP)	256:292	It is based on the use of a molecularly imprinted polymer (MIP) and of dsDNA as a bio-specific substance.
31745651	2	45	theme	bio-specific	312:323	arg1	substance					325:333	a bio-specific substance	310:333	a bio-specific substance	310:333	It is based on the use of a molecularly imprinted polymer (MIP) and of dsDNA as a bio-specific substance.
31745651	11	46	theme	fabrication	1374:1384	arg1	representation					1352:1365	Graphical abstractSchematic representation	1324:1365	Graphical abstractSchematic representation of the fabrication of an electrochemical sensor for determination of 2,4-dichlorophenoxyacetic acid (2,4-D).	1324:1474	Graphical abstractSchematic representation of the fabrication of an electrochemical sensor for determination of 2,4-dichlorophenoxyacetic acid (2,4-D).
31745651	7	47	theme	pulse	948:952	arg1	voltammetry					954:964	differential pulse voltammetry	935:964	differential pulse voltammetry (DPV)	935:970	After rebinding 2,4-D, the decrease in the peak current of oxidation of iron(II) acting as an electrochemical redox probe was measured by differential pulse voltammetry (DPV).
31745651	7	47	theme	pulse	948:952	arg1	DPV					967:969	DPV	967:969	DPV	967:969	After rebinding 2,4-D, the decrease in the peak current of oxidation of iron(II) acting as an electrochemical redox probe was measured by differential pulse voltammetry (DPV).
31745651	7	48	theme	electrochemical	891:905	arg1	probe					913:917	an electrochemical redox probe	888:917	an electrochemical redox probe	888:917	After rebinding 2,4-D, the decrease in the peak current of oxidation of iron(II) acting as an electrochemical redox probe was measured by differential pulse voltammetry (DPV).
31745651	6	49	from	adsorption	702:711	arg1	electrode					720:728	the electrode	716:728	the electrode	716:728	The interaction of DNA with 2,4-D leads to its adsorption on the electrode, and this increases the sensitivity and selectivity of the method.
31745651	10	50	theme	environmental	1229:1241	arg1	water					1243:1247	spiked environmental water and soil samples	1222:1264	water	1243:1247	The modified electrode was applied to quantify 2,4-D in spiked environmental water and soil samples and gave absolute recoveries varying from 91.5 to 109.0%.
31745651	7	51	theme	redox	907:911	arg1	probe					913:917	an electrochemical redox probe	888:917	an electrochemical redox probe	888:917	After rebinding 2,4-D, the decrease in the peak current of oxidation of iron(II) acting as an electrochemical redox probe was measured by differential pulse voltammetry (DPV).
31745651	4	52	theme	carbon	586:591	arg1	MWCNTs					604:609	MWCNTs	604:609	MWCNTs	604:609	The imprinted MIP was placed on a pencil graphite electrode (PGE) modified with chitosan and multiwalled carbon nanotubes (MWCNTs).
31745651	4	52	theme	carbon	586:591	arg1	nanotubes					593:601	multiwalled carbon nanotubes	574:601	multiwalled carbon nanotubes (MWCNTs)	574:610	The imprinted MIP was placed on a pencil graphite electrode (PGE) modified with chitosan and multiwalled carbon nanotubes (MWCNTs).
31745651	0	53	theme	electrochemical	49:63	arg1	sensing					65:71	electrochemical sensing	49:71	electrochemical sensing of 2,4-dichlorophenoxyacetic acid	49:105	An imprinted polymeric matrix containing DNA for electrochemical sensing of 2,4-dichlorophenoxyacetic acid.
31745651	10	54	theme	spiked	1222:1227	arg1	water					1243:1247	spiked environmental water and soil samples	1222:1264	water	1243:1247	The modified electrode was applied to quantify 2,4-D in spiked environmental water and soil samples and gave absolute recoveries varying from 91.5 to 109.0%.
31745651	8	55	theme	concentration	1068:1080	arg1	range					1082:1086	the 0.01 to 10 pM 2,4-D concentration range	1044:1086	the 0.01 to 10 pM 2,4-D concentration range	1044:1086	The current, typically measured at around 0.5 V, increases linearly in the 0.01 to 10 pM 2,4-D concentration range, and the detection limit is 4.0 fM.
31745651	11	56	theme	electrochemical	1392:1406	arg1	sensor					1408:1413	an electrochemical sensor	1389:1413	an electrochemical sensor for determination of 2,4-dichlorophenoxyacetic acid (2,4-D)	1389:1473	Graphical abstractSchematic representation of the fabrication of an electrochemical sensor for determination of 2,4-dichlorophenoxyacetic acid (2,4-D).
31745651	4	57	theme	multiwalled	574:584	arg1	MWCNTs					604:609	MWCNTs	604:609	MWCNTs	604:609	The imprinted MIP was placed on a pencil graphite electrode (PGE) modified with chitosan and multiwalled carbon nanotubes (MWCNTs).
31745651	4	57	theme	multiwalled	574:584	arg1	nanotubes					593:601	multiwalled carbon nanotubes	574:601	multiwalled carbon nanotubes (MWCNTs)	574:610	The imprinted MIP was placed on a pencil graphite electrode (PGE) modified with chitosan and multiwalled carbon nanotubes (MWCNTs).
30698577	3	0	attach	isolated	397:404	arg1	grains					425:430	Tibetan Kefir grains	411:430	Tibetan Kefir grains	411:430	In this study, the antidepressant effect of Lactobacillus kefiranofaciens CGMCC2809 (ZW3) isolated from Tibetan Kefir grains was investigated using a mouse model of chronic unpredictable mild stress (CUMS).
30698577	3	0	attach	isolated	397:404	arg2	ZW3					392:394	ZW3	392:394	ZW3	392:394	In this study, the antidepressant effect of Lactobacillus kefiranofaciens CGMCC2809 (ZW3) isolated from Tibetan Kefir grains was investigated using a mouse model of chronic unpredictable mild stress (CUMS).
30698577	3	0	attach	isolated	397:404	arg2	kefiranofaciens					365:379	kefiranofaciens	365:379	kefiranofaciens	365:379	In this study, the antidepressant effect of Lactobacillus kefiranofaciens CGMCC2809 (ZW3) isolated from Tibetan Kefir grains was investigated using a mouse model of chronic unpredictable mild stress (CUMS).
30698577	7	1	attach	present	947:953	arg2	present					947:953	present	947:953	present	947:953	We found that the probiotic strain was present in the whole intestine, even 7 days after its administration was stopped.
30698577	7	1	attach	present	947:953	arg2	strain					936:941	the probiotic strain	922:941	the probiotic strain	922:941	We found that the probiotic strain was present in the whole intestine, even 7 days after its administration was stopped.
30698577	7	1	attach	present	947:953	arg1	intestine					968:976	the whole intestine	958:976	the whole intestine	958:976	We found that the probiotic strain was present in the whole intestine, even 7 days after its administration was stopped.
30698577	5	2	from	disorders	643:651	arg1	axis					691:694	the hypothalamic-pituitary-adrenal axis	656:694	the hypothalamic-pituitary-adrenal axis	656:694	Moreover, ZW3 regulated biochemical disorders in the hypothalamic-pituitary-adrenal axis, immune system and tryptophan metabolism caused by stress.
30698577	4	3	theme	depression-like	527:541	arg1	behavior					543:550	depression-like behavior	527:550	depression-like behavior	527:550	ZW3 improved depression-like behavior and independent exploration ability in the CUMS group.
30698577	4	4	theme	independent	556:566	arg1	ability					580:586	independent exploration ability	556:586	independent exploration ability	556:586	ZW3 improved depression-like behavior and independent exploration ability in the CUMS group.
30698577	5	5	theme	tryptophan	715:724	arg1	metabolism					726:735	tryptophan metabolism	715:735	tryptophan metabolism	715:735	Moreover, ZW3 regulated biochemical disorders in the hypothalamic-pituitary-adrenal axis, immune system and tryptophan metabolism caused by stress.
30698577	7	6	theme	probiotic	926:934	arg1	present					947:953	present	947:953	present	947:953	We found that the probiotic strain was present in the whole intestine, even 7 days after its administration was stopped.
30698577	7	6	theme	probiotic	926:934	arg1	strain					936:941	the probiotic strain	922:941	the probiotic strain	922:941	We found that the probiotic strain was present in the whole intestine, even 7 days after its administration was stopped.
30698577	7	7	located	present	947:953	arg2	present					947:953	present	947:953	present	947:953	We found that the probiotic strain was present in the whole intestine, even 7 days after its administration was stopped.
30698577	7	7	located	present	947:953	arg2	strain					936:941	the probiotic strain	922:941	the probiotic strain	922:941	We found that the probiotic strain was present in the whole intestine, even 7 days after its administration was stopped.
30698577	7	7	located	present	947:953	arg1	intestine					968:976	the whole intestine	958:976	the whole intestine	958:976	We found that the probiotic strain was present in the whole intestine, even 7 days after its administration was stopped.
30698577	6	8	theme	water	876:880	arg1	content					882:888	the fecal water content	866:888	the fecal water content in stressed mice	866:905	Furthermore, ZW3 could modulate the composition of the gut microbiota, and alleviate constipation by improving the fecal water content in stressed mice.
30698577	0	9	theme	stressed	111:118	arg1	mice					120:123	stressed mice	111:123	stressed mice	111:123	Supplementation with Lactobacillus kefiranofaciens ZW3 from Tibetan Kefir improves depression-like behavior in stressed mice by modulating the gut microbiota.
30698577	6	10	theme	fecal	870:874	arg1	content					882:888	the fecal water content	866:888	the fecal water content in stressed mice	866:905	Furthermore, ZW3 could modulate the composition of the gut microbiota, and alleviate constipation by improving the fecal water content in stressed mice.
30698577	5	11	theme	hypothalamic-pituitary-adrenal	660:689	arg1	axis					691:694	the hypothalamic-pituitary-adrenal axis	656:694	the hypothalamic-pituitary-adrenal axis	656:694	Moreover, ZW3 regulated biochemical disorders in the hypothalamic-pituitary-adrenal axis, immune system and tryptophan metabolism caused by stress.
30698577	3	12	theme	chronic	472:478	arg1	stress					499:504	chronic unpredictable mild stress	472:504	chronic unpredictable mild stress (CUMS)	472:511	In this study, the antidepressant effect of Lactobacillus kefiranofaciens CGMCC2809 (ZW3) isolated from Tibetan Kefir grains was investigated using a mouse model of chronic unpredictable mild stress (CUMS).
30698577	3	12	theme	chronic	472:478	arg1	CUMS					507:510	CUMS	507:510	CUMS	507:510	In this study, the antidepressant effect of Lactobacillus kefiranofaciens CGMCC2809 (ZW3) isolated from Tibetan Kefir grains was investigated using a mouse model of chronic unpredictable mild stress (CUMS).
30698577	7	13	from	present	947:953	arg1	intestine					968:976	the whole intestine	958:976	the whole intestine	958:976	We found that the probiotic strain was present in the whole intestine, even 7 days after its administration was stopped.
30698577	3	14	theme	unpredictable	480:492	arg1	stress					499:504	chronic unpredictable mild stress	472:504	chronic unpredictable mild stress (CUMS)	472:511	In this study, the antidepressant effect of Lactobacillus kefiranofaciens CGMCC2809 (ZW3) isolated from Tibetan Kefir grains was investigated using a mouse model of chronic unpredictable mild stress (CUMS).
30698577	3	14	theme	unpredictable	480:492	arg1	CUMS					507:510	CUMS	507:510	CUMS	507:510	In this study, the antidepressant effect of Lactobacillus kefiranofaciens CGMCC2809 (ZW3) isolated from Tibetan Kefir grains was investigated using a mouse model of chronic unpredictable mild stress (CUMS).
30698577	3	15	theme	kefiranofaciens	365:379	arg1	effect					341:346	the antidepressant effect	322:346	the antidepressant effect of Lactobacillus kefiranofaciens CGMCC2809 (ZW3) isolated from Tibetan Kefir grains	322:430	In this study, the antidepressant effect of Lactobacillus kefiranofaciens CGMCC2809 (ZW3) isolated from Tibetan Kefir grains was investigated using a mouse model of chronic unpredictable mild stress (CUMS).
30698577	5	16	theme	immune	697:702	arg1	system					704:709	immune system	697:709	immune system	697:709	Moreover, ZW3 regulated biochemical disorders in the hypothalamic-pituitary-adrenal axis, immune system and tryptophan metabolism caused by stress.
30698577	6	17	from	content	882:888	arg1	mice					902:905	stressed mice	893:905	stressed mice	893:905	Furthermore, ZW3 could modulate the composition of the gut microbiota, and alleviate constipation by improving the fecal water content in stressed mice.
30698577	8	18	theme	kefiranofaciens	1059:1073	arg1	ZW3					1075:1077	L. kefiranofaciens ZW3	1056:1077	L. kefiranofaciens ZW3	1056:1077	These results suggest that L. kefiranofaciens ZW3 might improve depression by regulating the gut microbiota as a probiotic food.
30698577	3	19	theme	Tibetan	411:417	arg1	grains					425:430	Tibetan Kefir grains	411:430	Tibetan Kefir grains	411:430	In this study, the antidepressant effect of Lactobacillus kefiranofaciens CGMCC2809 (ZW3) isolated from Tibetan Kefir grains was investigated using a mouse model of chronic unpredictable mild stress (CUMS).
30698577	4	20	theme	CUMS	595:598	arg1	group					600:604	the CUMS group	591:604	the CUMS group	591:604	ZW3 improved depression-like behavior and independent exploration ability in the CUMS group.
30698577	0	21	theme	gut	143:145	arg1	microbiota					147:156	the gut microbiota	139:156	the gut microbiota	139:156	Supplementation with Lactobacillus kefiranofaciens ZW3 from Tibetan Kefir improves depression-like behavior in stressed mice by modulating the gut microbiota.
30698577	5	22	theme	biochemical	631:641	arg1	disorders					643:651	biochemical disorders	631:651	biochemical disorders in the hypothalamic-pituitary-adrenal axis	631:694	Moreover, ZW3 regulated biochemical disorders in the hypothalamic-pituitary-adrenal axis, immune system and tryptophan metabolism caused by stress.
30698577	1	23	theme	depression-like	229:243	arg1	behavior					245:252	depression-like behavior	229:252	depression-like behavior	229:252	Increasing evidence indicates that probiotics can effectively improve depression-like behavior.
30698577	3	24	theme	Kefir	419:423	arg1	grains					425:430	Tibetan Kefir grains	411:430	Tibetan Kefir grains	411:430	In this study, the antidepressant effect of Lactobacillus kefiranofaciens CGMCC2809 (ZW3) isolated from Tibetan Kefir grains was investigated using a mouse model of chronic unpredictable mild stress (CUMS).
30698577	7	25	from	intestine	968:976	arg1	present					947:953	present	947:953	present	947:953	We found that the probiotic strain was present in the whole intestine, even 7 days after its administration was stopped.
30698577	7	25	from	intestine	968:976	arg1	strain					936:941	the probiotic strain	922:941	the probiotic strain	922:941	We found that the probiotic strain was present in the whole intestine, even 7 days after its administration was stopped.
30698577	8	26	theme	gut	1122:1124	arg1	microbiota					1126:1135	the gut microbiota	1118:1135	the gut microbiota	1118:1135	These results suggest that L. kefiranofaciens ZW3 might improve depression by regulating the gut microbiota as a probiotic food.
30698577	8	26	theme	gut	1122:1124	arg1	food					1152:1155	a probiotic food	1140:1155	a probiotic food	1140:1155	These results suggest that L. kefiranofaciens ZW3 might improve depression by regulating the gut microbiota as a probiotic food.
30698577	8	27	theme	probiotic	1142:1150	arg1	microbiota					1126:1135	the gut microbiota	1118:1135	the gut microbiota	1118:1135	These results suggest that L. kefiranofaciens ZW3 might improve depression by regulating the gut microbiota as a probiotic food.
30698577	8	27	theme	probiotic	1142:1150	arg1	food					1152:1155	a probiotic food	1140:1155	a probiotic food	1140:1155	These results suggest that L. kefiranofaciens ZW3 might improve depression by regulating the gut microbiota as a probiotic food.
30698577	0	28	from	behavior	99:106	arg1	mice					120:123	stressed mice	111:123	stressed mice	111:123	Supplementation with Lactobacillus kefiranofaciens ZW3 from Tibetan Kefir improves depression-like behavior in stressed mice by modulating the gut microbiota.
30698577	3	29	theme	mild	494:497	arg1	stress					499:504	chronic unpredictable mild stress	472:504	chronic unpredictable mild stress (CUMS)	472:511	In this study, the antidepressant effect of Lactobacillus kefiranofaciens CGMCC2809 (ZW3) isolated from Tibetan Kefir grains was investigated using a mouse model of chronic unpredictable mild stress (CUMS).
30698577	3	29	theme	mild	494:497	arg1	CUMS					507:510	CUMS	507:510	CUMS	507:510	In this study, the antidepressant effect of Lactobacillus kefiranofaciens CGMCC2809 (ZW3) isolated from Tibetan Kefir grains was investigated using a mouse model of chronic unpredictable mild stress (CUMS).
30698577	3	30	theme	mouse	457:461	arg1	model					463:467	a mouse model	455:467	a mouse model of chronic unpredictable mild stress (CUMS)	455:511	In this study, the antidepressant effect of Lactobacillus kefiranofaciens CGMCC2809 (ZW3) isolated from Tibetan Kefir grains was investigated using a mouse model of chronic unpredictable mild stress (CUMS).
30698577	5	31	from	metabolism	726:735	arg1	axis					691:694	the hypothalamic-pituitary-adrenal axis	656:694	the hypothalamic-pituitary-adrenal axis	656:694	Moreover, ZW3 regulated biochemical disorders in the hypothalamic-pituitary-adrenal axis, immune system and tryptophan metabolism caused by stress.
30698577	2	32	theme	underlying	268:277	arg1	mechanism					279:287	the underlying mechanism	264:287	the underlying mechanism	264:287	However, the underlying mechanism is still unclear.
30698577	2	32	theme	underlying	268:277	arg1	unclear					298:304	unclear	298:304	unclear	298:304	However, the underlying mechanism is still unclear.
30698577	3	33	theme	stress	499:504	arg1	model					463:467	a mouse model	455:467	a mouse model of chronic unpredictable mild stress (CUMS)	455:511	In this study, the antidepressant effect of Lactobacillus kefiranofaciens CGMCC2809 (ZW3) isolated from Tibetan Kefir grains was investigated using a mouse model of chronic unpredictable mild stress (CUMS).
30698577	4	34	theme	exploration	568:578	arg1	ability					580:586	independent exploration ability	556:586	independent exploration ability	556:586	ZW3 improved depression-like behavior and independent exploration ability in the CUMS group.
30698577	5	35	from	system	704:709	arg1	axis					691:694	the hypothalamic-pituitary-adrenal axis	656:694	the hypothalamic-pituitary-adrenal axis	656:694	Moreover, ZW3 regulated biochemical disorders in the hypothalamic-pituitary-adrenal axis, immune system and tryptophan metabolism caused by stress.
30698577	6	36	theme	microbiota	814:823	arg1	composition					791:801	the composition	787:801	the composition of the gut microbiota	787:823	Furthermore, ZW3 could modulate the composition of the gut microbiota, and alleviate constipation by improving the fecal water content in stressed mice.
30698577	7	37	theme	whole	962:966	arg1	intestine					968:976	the whole intestine	958:976	the whole intestine	958:976	We found that the probiotic strain was present in the whole intestine, even 7 days after its administration was stopped.
30698577	8	38	theme	L.	1056:1057	arg1	ZW3					1075:1077	L. kefiranofaciens ZW3	1056:1077	L. kefiranofaciens ZW3	1056:1077	These results suggest that L. kefiranofaciens ZW3 might improve depression by regulating the gut microbiota as a probiotic food.
30698577	0	39	with	Supplementation	0:14	arg1	kefiranofaciens					35:49	Lactobacillus kefiranofaciens	21:49	Lactobacillus kefiranofaciens ZW3 from Tibetan Kefir	21:72	Supplementation with Lactobacillus kefiranofaciens ZW3 from Tibetan Kefir improves depression-like behavior in stressed mice by modulating the gut microbiota.
30698577	6	40	theme	gut	810:812	arg1	microbiota					814:823	the gut microbiota	806:823	the gut microbiota	806:823	Furthermore, ZW3 could modulate the composition of the gut microbiota, and alleviate constipation by improving the fecal water content in stressed mice.
30698577	3	41	theme	antidepressant	326:339	arg1	effect					341:346	the antidepressant effect	322:346	the antidepressant effect of Lactobacillus kefiranofaciens CGMCC2809 (ZW3) isolated from Tibetan Kefir grains	322:430	In this study, the antidepressant effect of Lactobacillus kefiranofaciens CGMCC2809 (ZW3) isolated from Tibetan Kefir grains was investigated using a mouse model of chronic unpredictable mild stress (CUMS).
30698577	6	42	theme	stressed	893:900	arg1	mice					902:905	stressed mice	893:905	stressed mice	893:905	Furthermore, ZW3 could modulate the composition of the gut microbiota, and alleviate constipation by improving the fecal water content in stressed mice.
30698577	0	43	theme	depression-like	83:97	arg1	behavior					99:106	depression-like behavior	83:106	depression-like behavior in stressed mice	83:123	Supplementation with Lactobacillus kefiranofaciens ZW3 from Tibetan Kefir improves depression-like behavior in stressed mice by modulating the gut microbiota.
30698577	1	44	theme	Increasing	159:168	arg1	evidence					170:177	Increasing evidence	159:177	Increasing evidence	159:177	Increasing evidence indicates that probiotics can effectively improve depression-like behavior.
30698577	0	45	theme	Lactobacillus	21:33	arg1	kefiranofaciens					35:49	Lactobacillus kefiranofaciens	21:49	Lactobacillus kefiranofaciens ZW3 from Tibetan Kefir	21:72	Supplementation with Lactobacillus kefiranofaciens ZW3 from Tibetan Kefir improves depression-like behavior in stressed mice by modulating the gut microbiota.
30914712	6	0	with	SOX10	961:965	arg1	markers					1048:1054	melanocyte-specific markers	1028:1054	melanocyte-specific markers	1028:1054	By analyzing mRNA expression of TYR, SOX10, and MITF, melanosome structure, and immunofluorescence with melanocyte-specific markers, we revealed that the SP-fraction contained MelSCs with an admixture of differentiated melanocytes.
30914712	3	1	theme	human	624:628	arg1	HuHF					646:649	HuHF	646:649	HuHF	646:649	It would be beneficial to isolate MelSCs in order to further study their function in pigmentary disorders; however, due to the lack of specific molecular surface markers, this has not yet been successfully accomplished in human hair follicles (HuHF).
30914712	3	1	theme	human	624:628	arg1	follicles					635:643	human hair follicles	624:643	human hair follicles (HuHF)	624:650	It would be beneficial to isolate MelSCs in order to further study their function in pigmentary disorders; however, due to the lack of specific molecular surface markers, this has not yet been successfully accomplished in human hair follicles (HuHF).
30914712	3	2	theme	pigmentary	487:496	arg1	disorders					498:506	pigmentary disorders	487:506	pigmentary disorders	487:506	It would be beneficial to isolate MelSCs in order to further study their function in pigmentary disorders; however, due to the lack of specific molecular surface markers, this has not yet been successfully accomplished in human hair follicles (HuHF).
30914712	6	3	theme	differentiated	1128:1141	arg1	melanocytes					1143:1153	differentiated melanocytes	1128:1153	differentiated melanocytes	1128:1153	By analyzing mRNA expression of TYR, SOX10, and MITF, melanosome structure, and immunofluorescence with melanocyte-specific markers, we revealed that the SP-fraction contained MelSCs with an admixture of differentiated melanocytes.
30914712	4	4	theme	hematopoietic	767:779	arg1	cells					796:800	hematopoietic and tumor stem cells	767:800	cells	796:800	One potential method for MelSCs isolation is the "side population" technique, which is frequently used to isolate hematopoietic and tumor stem cells.
30914712	4	5	theme	population	708:717	arg1	method					667:672	One potential method	653:672	One potential method for MelSCs isolation	653:693	One potential method for MelSCs isolation is the "side population" technique, which is frequently used to isolate hematopoietic and tumor stem cells.
30914712	4	5	theme	population	708:717	arg1	technique					720:728	the "side population" technique	698:728	the "side population" technique	698:728	One potential method for MelSCs isolation is the "side population" technique, which is frequently used to isolate hematopoietic and tumor stem cells.
30914712	5	6	theme	population	871:880	arg1	"					881:881	"side population"	865:881	"side population" to investigate their melanotic function	865:921	In the present study, we decided to isolate HuHF MelSCs using "side population" to investigate their melanotic function.
30914712	8	7	used	used	1474:1477	arg2	MelSCs					1443:1448	HuHF MelSCs	1438:1448	HuHF MelSCs that can potentially be used to repopulate skin for pigmentation	1438:1513	In summary, the SP technique is capable of isolating HuHF MelSCs that can potentially be used to repopulate skin for pigmentation.
30914712	7	8	theme	in	1173:1174	arg1	studies					1181:1187	our in vivo studies	1169:1187	our in vivo studies	1169:1187	Furthermore, our in vivo studies indicated that differentiated SP-fraction cells, when fabricated into a cell-chitosan/gelatin composite, could transiently repopulate immunologically compromised mice skin to regain pigmentation.
30914712	4	9	theme	side	703:706	arg1	method					667:672	One potential method	653:672	One potential method for MelSCs isolation	653:693	One potential method for MelSCs isolation is the "side population" technique, which is frequently used to isolate hematopoietic and tumor stem cells.
30914712	4	9	theme	side	703:706	arg1	technique					720:728	the "side population" technique	698:728	the "side population" technique	698:728	One potential method for MelSCs isolation is the "side population" technique, which is frequently used to isolate hematopoietic and tumor stem cells.
30914712	6	10	with	structure	989:997	arg1	markers					1048:1054	melanocyte-specific markers	1028:1054	melanocyte-specific markers	1028:1054	By analyzing mRNA expression of TYR, SOX10, and MITF, melanosome structure, and immunofluorescence with melanocyte-specific markers, we revealed that the SP-fraction contained MelSCs with an admixture of differentiated melanocytes.
30914712	4	11	theme	tumor	785:789	arg1	cells					796:800	hematopoietic and tumor stem cells	767:800	cells	796:800	One potential method for MelSCs isolation is the "side population" technique, which is frequently used to isolate hematopoietic and tumor stem cells.
30914712	6	12	theme	SOX10	961:965	arg1	expression					942:951	mRNA expression	937:951	mRNA expression of TYR, SOX10, and MITF, melanosome structure, and immunofluorescence with melanocyte-specific markers	937:1054	By analyzing mRNA expression of TYR, SOX10, and MITF, melanosome structure, and immunofluorescence with melanocyte-specific markers, we revealed that the SP-fraction contained MelSCs with an admixture of differentiated melanocytes.
30914712	2	13	theme	bulge/sub-bulge	273:287	arg1	region					289:294	the bulge/sub-bulge region	269:294	the bulge/sub-bulge region of the lower permanent portion of the hair follicle	269:346	Melanocyte stem cells (MelSCs) and melanocyte progenitors reside in the bulge/sub-bulge region of the lower permanent portion of the hair follicle and play a vital role for repigmentation in vitiligo.
30914712	6	14	theme	MITF	972:975	arg1	expression					942:951	mRNA expression	937:951	mRNA expression of TYR, SOX10, and MITF, melanosome structure, and immunofluorescence with melanocyte-specific markers	937:1054	By analyzing mRNA expression of TYR, SOX10, and MITF, melanosome structure, and immunofluorescence with melanocyte-specific markers, we revealed that the SP-fraction contained MelSCs with an admixture of differentiated melanocytes.
30914712	6	15	theme	melanosome	978:987	arg1	structure					989:997	melanosome structure	978:997	melanosome structure	978:997	By analyzing mRNA expression of TYR, SOX10, and MITF, melanosome structure, and immunofluorescence with melanocyte-specific markers, we revealed that the SP-fraction contained MelSCs with an admixture of differentiated melanocytes.
30914712	8	16	theme	HuHF	1438:1441	arg1	MelSCs					1443:1448	HuHF MelSCs	1438:1448	HuHF MelSCs that can potentially be used to repopulate skin for pigmentation	1438:1513	In summary, the SP technique is capable of isolating HuHF MelSCs that can potentially be used to repopulate skin for pigmentation.
30914712	6	17	with	immunofluorescence	1004:1021	arg1	markers					1048:1054	melanocyte-specific markers	1028:1054	melanocyte-specific markers	1028:1054	By analyzing mRNA expression of TYR, SOX10, and MITF, melanosome structure, and immunofluorescence with melanocyte-specific markers, we revealed that the SP-fraction contained MelSCs with an admixture of differentiated melanocytes.
30914712	6	18	theme	melanocyte-specific	1028:1046	arg1	markers					1048:1054	melanocyte-specific markers	1028:1054	melanocyte-specific markers	1028:1054	By analyzing mRNA expression of TYR, SOX10, and MITF, melanosome structure, and immunofluorescence with melanocyte-specific markers, we revealed that the SP-fraction contained MelSCs with an admixture of differentiated melanocytes.
30914712	4	19	theme	"	718:718	arg1	method					667:672	One potential method	653:672	One potential method for MelSCs isolation	653:693	One potential method for MelSCs isolation is the "side population" technique, which is frequently used to isolate hematopoietic and tumor stem cells.
30914712	4	19	theme	"	718:718	arg1	technique					720:728	the "side population" technique	698:728	the "side population" technique	698:728	One potential method for MelSCs isolation is the "side population" technique, which is frequently used to isolate hematopoietic and tumor stem cells.
30914712	4	20	used	used	751:754	arg2	technique					720:728	the "side population" technique	698:728	the "side population" technique	698:728	One potential method for MelSCs isolation is the "side population" technique, which is frequently used to isolate hematopoietic and tumor stem cells.
30914712	4	20	used	used	751:754	arg2	method					667:672	One potential method	653:672	One potential method for MelSCs isolation	653:693	One potential method for MelSCs isolation is the "side population" technique, which is frequently used to isolate hematopoietic and tumor stem cells.
30914712	0	21	theme	Chitosan/Gelatin	11:26	arg1	Fabrication					38:48	Melanocyte Chitosan/Gelatin Composite Fabrication	0:48	Melanocyte Chitosan/Gelatin Composite Fabrication with Human Outer Root Sheath-Derived Cells	0:91	Melanocyte Chitosan/Gelatin Composite Fabrication with Human Outer Root Sheath-Derived Cells to Produce Pigment.
30914712	8	22	from	capable	1417:1423	arg1	summary					1388:1394	summary	1388:1394	summary	1388:1394	In summary, the SP technique is capable of isolating HuHF MelSCs that can potentially be used to repopulate skin for pigmentation.
30914712	2	23	theme	portion	319:325	arg1	region					289:294	the bulge/sub-bulge region	269:294	the bulge/sub-bulge region of the lower permanent portion of the hair follicle	269:346	Melanocyte stem cells (MelSCs) and melanocyte progenitors reside in the bulge/sub-bulge region of the lower permanent portion of the hair follicle and play a vital role for repigmentation in vitiligo.
30914712	6	24	theme	structure	989:997	arg1	expression					942:951	mRNA expression	937:951	mRNA expression of TYR, SOX10, and MITF, melanosome structure, and immunofluorescence with melanocyte-specific markers	937:1054	By analyzing mRNA expression of TYR, SOX10, and MITF, melanosome structure, and immunofluorescence with melanocyte-specific markers, we revealed that the SP-fraction contained MelSCs with an admixture of differentiated melanocytes.
30914712	1	25	theme	skin	182:185	arg1	pigmentation					187:198	skin pigmentation	182:198	skin pigmentation	182:198	The hair follicle serves as a melanocyte reservoir for both hair and skin pigmentation.
30914712	0	26	theme	Melanocyte	0:9	arg1	Fabrication					38:48	Melanocyte Chitosan/Gelatin Composite Fabrication	0:48	Melanocyte Chitosan/Gelatin Composite Fabrication with Human Outer Root Sheath-Derived Cells	0:91	Melanocyte Chitosan/Gelatin Composite Fabrication with Human Outer Root Sheath-Derived Cells to Produce Pigment.
30914712	7	27	theme	compromised	1339:1349	arg1	mice					1351:1354	immunologically compromised mice	1323:1354	immunologically compromised mice	1323:1354	Furthermore, our in vivo studies indicated that differentiated SP-fraction cells, when fabricated into a cell-chitosan/gelatin composite, could transiently repopulate immunologically compromised mice skin to regain pigmentation.
30914712	4	28	theme	stem	791:794	arg1	cells					796:800	hematopoietic and tumor stem cells	767:800	cells	796:800	One potential method for MelSCs isolation is the "side population" technique, which is frequently used to isolate hematopoietic and tumor stem cells.
30914712	2	29	theme	permanent	309:317	arg1	portion					319:325	the lower permanent portion	299:325	the lower permanent portion of the hair follicle	299:346	Melanocyte stem cells (MelSCs) and melanocyte progenitors reside in the bulge/sub-bulge region of the lower permanent portion of the hair follicle and play a vital role for repigmentation in vitiligo.
30914712	5	30	theme	present	810:816	arg1	study					818:822	the present study	806:822	the present study	806:822	In the present study, we decided to isolate HuHF MelSCs using "side population" to investigate their melanotic function.
30914712	3	31	from	function	475:482	arg1	disorders					498:506	pigmentary disorders	487:506	pigmentary disorders	487:506	It would be beneficial to isolate MelSCs in order to further study their function in pigmentary disorders; however, due to the lack of specific molecular surface markers, this has not yet been successfully accomplished in human hair follicles (HuHF).
30914712	2	32	theme	lower	303:307	arg1	portion					319:325	the lower permanent portion	299:325	the lower permanent portion of the hair follicle	299:346	Melanocyte stem cells (MelSCs) and melanocyte progenitors reside in the bulge/sub-bulge region of the lower permanent portion of the hair follicle and play a vital role for repigmentation in vitiligo.
30914712	0	33	theme	Composite	28:36	arg1	Fabrication					38:48	Melanocyte Chitosan/Gelatin Composite Fabrication	0:48	Melanocyte Chitosan/Gelatin Composite Fabrication with Human Outer Root Sheath-Derived Cells	0:91	Melanocyte Chitosan/Gelatin Composite Fabrication with Human Outer Root Sheath-Derived Cells to Produce Pigment.
30914712	6	34	with	MITF	972:975	arg1	markers					1048:1054	melanocyte-specific markers	1028:1054	melanocyte-specific markers	1028:1054	By analyzing mRNA expression of TYR, SOX10, and MITF, melanosome structure, and immunofluorescence with melanocyte-specific markers, we revealed that the SP-fraction contained MelSCs with an admixture of differentiated melanocytes.
30914712	1	35	theme	hair	117:120	arg1	reservoir					154:162	a melanocyte reservoir	141:162	a melanocyte reservoir for both hair and skin pigmentation	141:198	The hair follicle serves as a melanocyte reservoir for both hair and skin pigmentation.
30914712	1	35	theme	hair	117:120	arg1	follicle					122:129	The hair follicle	113:129	The hair follicle	113:129	The hair follicle serves as a melanocyte reservoir for both hair and skin pigmentation.
30914712	8	36	from	summary	1388:1394	arg1	capable					1417:1423	capable	1417:1423	capable	1417:1423	In summary, the SP technique is capable of isolating HuHF MelSCs that can potentially be used to repopulate skin for pigmentation.
30914712	8	36	from	summary	1388:1394	arg1	technique					1404:1412	the SP technique	1397:1412	the SP technique	1397:1412	In summary, the SP technique is capable of isolating HuHF MelSCs that can potentially be used to repopulate skin for pigmentation.
30914712	1	37	theme	melanocyte	143:152	arg1	reservoir					154:162	a melanocyte reservoir	141:162	a melanocyte reservoir for both hair and skin pigmentation	141:198	The hair follicle serves as a melanocyte reservoir for both hair and skin pigmentation.
30914712	1	37	theme	melanocyte	143:152	arg1	follicle					122:129	The hair follicle	113:129	The hair follicle	113:129	The hair follicle serves as a melanocyte reservoir for both hair and skin pigmentation.
30914712	0	38	theme	Human	55:59	arg1	Cells					87:91	Human Outer Root Sheath-Derived Cells	55:91	Human Outer Root Sheath-Derived Cells	55:91	Melanocyte Chitosan/Gelatin Composite Fabrication with Human Outer Root Sheath-Derived Cells to Produce Pigment.
30914712	4	39	theme	potential	657:665	arg1	method					667:672	One potential method	653:672	One potential method for MelSCs isolation	653:693	One potential method for MelSCs isolation is the "side population" technique, which is frequently used to isolate hematopoietic and tumor stem cells.
30914712	4	39	theme	potential	657:665	arg1	technique					720:728	the "side population" technique	698:728	the "side population" technique	698:728	One potential method for MelSCs isolation is the "side population" technique, which is frequently used to isolate hematopoietic and tumor stem cells.
30914712	8	40	theme	SP	1401:1402	arg1	capable					1417:1423	capable	1417:1423	capable	1417:1423	In summary, the SP technique is capable of isolating HuHF MelSCs that can potentially be used to repopulate skin for pigmentation.
30914712	8	40	theme	SP	1401:1402	arg1	technique					1404:1412	the SP technique	1397:1412	the SP technique	1397:1412	In summary, the SP technique is capable of isolating HuHF MelSCs that can potentially be used to repopulate skin for pigmentation.
30914712	3	41	theme	markers	564:570	arg1	lack					529:532	the lack	525:532	the lack of specific molecular surface markers	525:570	It would be beneficial to isolate MelSCs in order to further study their function in pigmentary disorders; however, due to the lack of specific molecular surface markers, this has not yet been successfully accomplished in human hair follicles (HuHF).
30914712	7	42	dep	in	1173:1174	arg1	vivo					1176:1179	vivo	1176:1179	vivo	1176:1179	Furthermore, our in vivo studies indicated that differentiated SP-fraction cells, when fabricated into a cell-chitosan/gelatin composite, could transiently repopulate immunologically compromised mice skin to regain pigmentation.
30914712	7	43	theme	cell-chitosan/gelatin	1261:1281	arg1	composite					1283:1291	a cell-chitosan/gelatin composite	1259:1291	a cell-chitosan/gelatin composite	1259:1291	Furthermore, our in vivo studies indicated that differentiated SP-fraction cells, when fabricated into a cell-chitosan/gelatin composite, could transiently repopulate immunologically compromised mice skin to regain pigmentation.
30914712	0	44	with	Fabrication	38:48	arg1	Cells					87:91	Human Outer Root Sheath-Derived Cells	55:91	Human Outer Root Sheath-Derived Cells	55:91	Melanocyte Chitosan/Gelatin Composite Fabrication with Human Outer Root Sheath-Derived Cells to Produce Pigment.
30914712	6	45	theme	TYR	956:958	arg1	expression					942:951	mRNA expression	937:951	mRNA expression of TYR, SOX10, and MITF, melanosome structure, and immunofluorescence with melanocyte-specific markers	937:1054	By analyzing mRNA expression of TYR, SOX10, and MITF, melanosome structure, and immunofluorescence with melanocyte-specific markers, we revealed that the SP-fraction contained MelSCs with an admixture of differentiated melanocytes.
30914712	0	46	theme	Root	67:70	arg1	Cells					87:91	Human Outer Root Sheath-Derived Cells	55:91	Human Outer Root Sheath-Derived Cells	55:91	Melanocyte Chitosan/Gelatin Composite Fabrication with Human Outer Root Sheath-Derived Cells to Produce Pigment.
30914712	3	47	theme	molecular	546:554	arg1	markers					564:570	specific molecular surface markers	537:570	specific molecular surface markers	537:570	It would be beneficial to isolate MelSCs in order to further study their function in pigmentary disorders; however, due to the lack of specific molecular surface markers, this has not yet been successfully accomplished in human hair follicles (HuHF).
30914712	2	48	from	role	365:368	arg1	vitiligo					392:399	vitiligo	392:399	vitiligo	392:399	Melanocyte stem cells (MelSCs) and melanocyte progenitors reside in the bulge/sub-bulge region of the lower permanent portion of the hair follicle and play a vital role for repigmentation in vitiligo.
30914712	2	49	theme	follicle	339:346	arg1	portion					319:325	the lower permanent portion	299:325	the lower permanent portion of the hair follicle	299:346	Melanocyte stem cells (MelSCs) and melanocyte progenitors reside in the bulge/sub-bulge region of the lower permanent portion of the hair follicle and play a vital role for repigmentation in vitiligo.
30914712	0	50	theme	Outer	61:65	arg1	Cells					87:91	Human Outer Root Sheath-Derived Cells	55:91	Human Outer Root Sheath-Derived Cells	55:91	Melanocyte Chitosan/Gelatin Composite Fabrication with Human Outer Root Sheath-Derived Cells to Produce Pigment.
30914712	3	51	theme	surface	556:562	arg1	markers					564:570	specific molecular surface markers	537:570	specific molecular surface markers	537:570	It would be beneficial to isolate MelSCs in order to further study their function in pigmentary disorders; however, due to the lack of specific molecular surface markers, this has not yet been successfully accomplished in human hair follicles (HuHF).
30914712	7	52	theme	differentiated	1204:1217	arg1	cells					1231:1235	differentiated SP-fraction cells	1204:1235	differentiated SP-fraction cells	1204:1235	Furthermore, our in vivo studies indicated that differentiated SP-fraction cells, when fabricated into a cell-chitosan/gelatin composite, could transiently repopulate immunologically compromised mice skin to regain pigmentation.
30914712	2	53	theme	hair	334:337	arg1	follicle					339:346	the hair follicle	330:346	the hair follicle	330:346	Melanocyte stem cells (MelSCs) and melanocyte progenitors reside in the bulge/sub-bulge region of the lower permanent portion of the hair follicle and play a vital role for repigmentation in vitiligo.
30914712	6	54	theme	melanocytes	1143:1153	arg1	admixture					1115:1123	an admixture	1112:1123	an admixture of differentiated melanocytes	1112:1153	By analyzing mRNA expression of TYR, SOX10, and MITF, melanosome structure, and immunofluorescence with melanocyte-specific markers, we revealed that the SP-fraction contained MelSCs with an admixture of differentiated melanocytes.
30914712	2	55	theme	stem	212:215	arg1	MelSCs					224:229	MelSCs	224:229	MelSCs	224:229	Melanocyte stem cells (MelSCs) and melanocyte progenitors reside in the bulge/sub-bulge region of the lower permanent portion of the hair follicle and play a vital role for repigmentation in vitiligo.
30914712	2	55	theme	stem	212:215	arg1	cells					217:221	Melanocyte stem cells	201:221	Melanocyte stem cells (MelSCs)	201:230	Melanocyte stem cells (MelSCs) and melanocyte progenitors reside in the bulge/sub-bulge region of the lower permanent portion of the hair follicle and play a vital role for repigmentation in vitiligo.
30914712	5	56	theme	melanotic	904:912	arg1	function					914:921	their melanotic function	898:921	their melanotic function	898:921	In the present study, we decided to isolate HuHF MelSCs using "side population" to investigate their melanotic function.
30914712	4	57	theme	MelSCs	678:683	arg1	isolation					685:693	MelSCs isolation	678:693	MelSCs isolation	678:693	One potential method for MelSCs isolation is the "side population" technique, which is frequently used to isolate hematopoietic and tumor stem cells.
30914712	5	58	theme	side	866:869	arg1	"					881:881	"side population"	865:881	"side population" to investigate their melanotic function	865:921	In the present study, we decided to isolate HuHF MelSCs using "side population" to investigate their melanotic function.
30914712	2	59	theme	Melanocyte	201:210	arg1	MelSCs					224:229	MelSCs	224:229	MelSCs	224:229	Melanocyte stem cells (MelSCs) and melanocyte progenitors reside in the bulge/sub-bulge region of the lower permanent portion of the hair follicle and play a vital role for repigmentation in vitiligo.
30914712	2	59	theme	Melanocyte	201:210	arg1	cells					217:221	Melanocyte stem cells	201:221	Melanocyte stem cells (MelSCs)	201:230	Melanocyte stem cells (MelSCs) and melanocyte progenitors reside in the bulge/sub-bulge region of the lower permanent portion of the hair follicle and play a vital role for repigmentation in vitiligo.
30914712	6	60	theme	immunofluorescence	1004:1021	arg1	expression					942:951	mRNA expression	937:951	mRNA expression of TYR, SOX10, and MITF, melanosome structure, and immunofluorescence with melanocyte-specific markers	937:1054	By analyzing mRNA expression of TYR, SOX10, and MITF, melanosome structure, and immunofluorescence with melanocyte-specific markers, we revealed that the SP-fraction contained MelSCs with an admixture of differentiated melanocytes.
30914712	6	61	contain	contained	1090:1098	arg2	MelSCs					1100:1105	MelSCs	1100:1105	MelSCs	1100:1105	By analyzing mRNA expression of TYR, SOX10, and MITF, melanosome structure, and immunofluorescence with melanocyte-specific markers, we revealed that the SP-fraction contained MelSCs with an admixture of differentiated melanocytes.
30914712	6	61	contain	contained	1090:1098	arg1	SP-fraction					1078:1088	the SP-fraction	1074:1088	the SP-fraction	1074:1088	By analyzing mRNA expression of TYR, SOX10, and MITF, melanosome structure, and immunofluorescence with melanocyte-specific markers, we revealed that the SP-fraction contained MelSCs with an admixture of differentiated melanocytes.
30914712	6	62	with	TYR	956:958	arg1	markers					1048:1054	melanocyte-specific markers	1028:1054	melanocyte-specific markers	1028:1054	By analyzing mRNA expression of TYR, SOX10, and MITF, melanosome structure, and immunofluorescence with melanocyte-specific markers, we revealed that the SP-fraction contained MelSCs with an admixture of differentiated melanocytes.
30914712	3	63	theme	hair	630:633	arg1	HuHF					646:649	HuHF	646:649	HuHF	646:649	It would be beneficial to isolate MelSCs in order to further study their function in pigmentary disorders; however, due to the lack of specific molecular surface markers, this has not yet been successfully accomplished in human hair follicles (HuHF).
30914712	3	63	theme	hair	630:633	arg1	follicles					635:643	human hair follicles	624:643	human hair follicles (HuHF)	624:650	It would be beneficial to isolate MelSCs in order to further study their function in pigmentary disorders; however, due to the lack of specific molecular surface markers, this has not yet been successfully accomplished in human hair follicles (HuHF).
30914712	2	64	theme	vital	359:363	arg1	role					365:368	a vital role	357:368	a vital role for repigmentation in vitiligo	357:399	Melanocyte stem cells (MelSCs) and melanocyte progenitors reside in the bulge/sub-bulge region of the lower permanent portion of the hair follicle and play a vital role for repigmentation in vitiligo.
30914712	6	65	theme	mRNA	937:940	arg1	expression					942:951	mRNA expression	937:951	mRNA expression of TYR, SOX10, and MITF, melanosome structure, and immunofluorescence with melanocyte-specific markers	937:1054	By analyzing mRNA expression of TYR, SOX10, and MITF, melanosome structure, and immunofluorescence with melanocyte-specific markers, we revealed that the SP-fraction contained MelSCs with an admixture of differentiated melanocytes.
30914712	0	66	theme	Sheath-Derived	72:85	arg1	Cells					87:91	Human Outer Root Sheath-Derived Cells	55:91	Human Outer Root Sheath-Derived Cells	55:91	Melanocyte Chitosan/Gelatin Composite Fabrication with Human Outer Root Sheath-Derived Cells to Produce Pigment.
30914712	2	67	theme	melanocyte	236:245	arg1	progenitors					247:257	melanocyte progenitors	236:257	melanocyte progenitors	236:257	Melanocyte stem cells (MelSCs) and melanocyte progenitors reside in the bulge/sub-bulge region of the lower permanent portion of the hair follicle and play a vital role for repigmentation in vitiligo.
30914712	3	68	theme	specific	537:544	arg1	markers					564:570	specific molecular surface markers	537:570	specific molecular surface markers	537:570	It would be beneficial to isolate MelSCs in order to further study their function in pigmentary disorders; however, due to the lack of specific molecular surface markers, this has not yet been successfully accomplished in human hair follicles (HuHF).
30914712	7	69	theme	SP-fraction	1219:1229	arg1	cells					1231:1235	differentiated SP-fraction cells	1204:1235	differentiated SP-fraction cells	1204:1235	Furthermore, our in vivo studies indicated that differentiated SP-fraction cells, when fabricated into a cell-chitosan/gelatin composite, could transiently repopulate immunologically compromised mice skin to regain pigmentation.
31253615	0	0	from	databases	21:29	arg1	age					38:40	the age	34:40	the age of big data	34:52	Nutrient composition databases in the age of big data: foodDB, a comprehensive, real-time database infrastructure.
31253615	9	1	theme	branded	1571:1577	arg1	foods					1579:1583	branded foods	1571:1583	branded foods	1571:1583	CONCLUSIONS foodDB is a powerful new tool for monitoring the food and drink marketplace, the comprehensive sampling and granularity of collection provides power for revealing analyses of the relationship between nutritional quality and marketing of branded foods, timely observation of product reformulation and other changes to the food marketplace.
31253615	7	2	theme	total	970:974	arg1	products					976:983	97 368 total products	963:983	97 368 total products	963:983	RESULTS Analyses using a single weekly timepoint of 97 368 total products in March 2018 identified 2699 ready meals and pizzas, and showed that lower price ready meals had significantly lower levels of fat, saturates, sugar and salt (p<0.001).
31253615	5	3	theme	supermarket	827:837	arg1	websites					839:846	the supermarket websites	823:846	the supermarket websites	823:846	METHODS Weekly extraction of nutrition and availability data of products was extracted from the webpages of the supermarket websites.
31253615	9	4	theme	other	1634:1638	arg1	changes					1640:1646	other changes	1634:1646	other changes to the food marketplace	1634:1670	CONCLUSIONS foodDB is a powerful new tool for monitoring the food and drink marketplace, the comprehensive sampling and granularity of collection provides power for revealing analyses of the relationship between nutritional quality and marketing of branded foods, timely observation of product reformulation and other changes to the food marketplace.
31253615	1	5	from	pace	248:251	arg1	marketplace					285:295	the food and drink marketplace	266:295	marketplace	285:295	OBJECTIVES Traditional methods for creating food composition tables struggle to cope with the large number of products and the rapid pace of change in the food and drink marketplace.
31253615	4	6	contain	has	611:613	arg2	observations					625:636	3 193 171 observations	615:636	3 193 171 observations of 128 283 distinct food and drink products measured at multiple timepoints	615:712	As of June 2018, foodDB has 3 193 171 observations of 128 283 distinct food and drink products measured at multiple timepoints.
31253615	4	6	contain	has	611:613	arg1	foodDB					604:609	foodDB	604:609	foodDB	604:609	As of June 2018, foodDB has 3 193 171 observations of 128 283 distinct food and drink products measured at multiple timepoints.
31253615	1	7	from	marketplace	285:295	arg1	number					215:220	the large number	205:220	the large number of products	205:232	OBJECTIVES Traditional methods for creating food composition tables struggle to cope with the large number of products and the rapid pace of change in the food and drink marketplace.
31253615	1	7	from	marketplace	285:295	arg1	pace					248:251	the rapid pace	238:251	the rapid pace of change in the food and drink marketplace	238:295	OBJECTIVES Traditional methods for creating food composition tables struggle to cope with the large number of products and the rapid pace of change in the food and drink marketplace.
31253615	4	8	theme	193	617:619	arg1	observations					625:636	3 193 171 observations	615:636	3 193 171 observations of 128 283 distinct food and drink products measured at multiple timepoints	615:712	As of June 2018, foodDB has 3 193 171 observations of 128 283 distinct food and drink products measured at multiple timepoints.
31253615	0	9	theme	comprehensive	65:77	arg1	foodDB					55:60	foodDB	55:60	foodDB	55:60	Nutrient composition databases in the age of big data: foodDB, a comprehensive, real-time database infrastructure.
31253615	0	9	theme	comprehensive	65:77	arg1	infrastructure					99:112	a comprehensive, real-time database infrastructure	63:112	a comprehensive, real-time database infrastructure	63:112	Nutrient composition databases in the age of big data: foodDB, a comprehensive, real-time database infrastructure.
31253615	8	10	theme	market	1306:1311	arg1	entries					1313:1319	new market entries	1302:1319	new market entries	1302:1319	Longitudinal analyses of 903 pizzas revealed that 10.8% changed their nutritional formulation over 6 months, and 29.9% were either discontinued or new market entries.
31253615	5	11	theme	Weekly	723:728	arg1	extraction					730:739	METHODS Weekly extraction	715:739	METHODS Weekly extraction of nutrition and availability data of products	715:786	METHODS Weekly extraction of nutrition and availability data of products was extracted from the webpages of the supermarket websites.
31253615	1	12	theme	rapid	242:246	arg1	pace					248:251	the rapid pace	238:251	the rapid pace of change in the food and drink marketplace	238:295	OBJECTIVES Traditional methods for creating food composition tables struggle to cope with the large number of products and the rapid pace of change in the food and drink marketplace.
31253615	5	13	theme	nutrition	744:752	arg1	extraction					730:739	METHODS Weekly extraction	715:739	METHODS Weekly extraction of nutrition and availability data of products	715:786	METHODS Weekly extraction of nutrition and availability data of products was extracted from the webpages of the supermarket websites.
31253615	5	14	dep	nutrition	744:752	arg1	data					771:774	data	771:774	data	771:774	METHODS Weekly extraction of nutrition and availability data of products was extracted from the webpages of the supermarket websites.
31253615	8	15	theme	nutritional	1225:1235	arg1	formulation					1237:1247	their nutritional formulation	1219:1247	their nutritional formulation	1219:1247	Longitudinal analyses of 903 pizzas revealed that 10.8% changed their nutritional formulation over 6 months, and 29.9% were either discontinued or new market entries.
31253615	9	16	theme	new	1355:1357	arg1	tool					1359:1362	a powerful new tool	1344:1362	a powerful new tool for monitoring the food and drink marketplace	1344:1408	CONCLUSIONS foodDB is a powerful new tool for monitoring the food and drink marketplace, the comprehensive sampling and granularity of collection provides power for revealing analyses of the relationship between nutritional quality and marketing of branded foods, timely observation of product reformulation and other changes to the food marketplace.
31253615	9	16	theme	new	1355:1357	arg1	foodDB					1334:1339	CONCLUSIONS foodDB	1322:1339	CONCLUSIONS foodDB	1322:1339	CONCLUSIONS foodDB is a powerful new tool for monitoring the food and drink marketplace, the comprehensive sampling and granularity of collection provides power for revealing analyses of the relationship between nutritional quality and marketing of branded foods, timely observation of product reformulation and other changes to the food marketplace.
31253615	9	17	theme	drink	1392:1396	arg1	marketplace					1398:1408	the food and drink marketplace	1379:1408	marketplace	1398:1408	CONCLUSIONS foodDB is a powerful new tool for monitoring the food and drink marketplace, the comprehensive sampling and granularity of collection provides power for revealing analyses of the relationship between nutritional quality and marketing of branded foods, timely observation of product reformulation and other changes to the food marketplace.
31253615	9	18	theme	foods	1579:1583	arg1	quality					1546:1552	nutritional quality	1534:1552	nutritional quality	1534:1552	CONCLUSIONS foodDB is a powerful new tool for monitoring the food and drink marketplace, the comprehensive sampling and granularity of collection provides power for revealing analyses of the relationship between nutritional quality and marketing of branded foods, timely observation of product reformulation and other changes to the food marketplace.
31253615	8	19	theme	Longitudinal	1155:1166	arg1	analyses					1168:1175	Longitudinal analyses	1155:1175	Longitudinal analyses of 903 pizzas	1155:1189	Longitudinal analyses of 903 pizzas revealed that 10.8% changed their nutritional formulation over 6 months, and 29.9% were either discontinued or new market entries.
31253615	0	20	dep	comprehensive	65:77	arg1	real-time					80:88	real-time	80:88	real-time	80:88	Nutrient composition databases in the age of big data: foodDB, a comprehensive, real-time database infrastructure.
31253615	1	21	theme	food	159:162	arg1	tables					176:181	food composition tables	159:181	food composition tables	159:181	OBJECTIVES Traditional methods for creating food composition tables struggle to cope with the large number of products and the rapid pace of change in the food and drink marketplace.
31253615	7	22	theme	fat	1113:1115	arg1	levels					1103:1108	significantly lower levels	1083:1108	significantly lower levels of fat, saturates, sugar and salt (p<0.001)	1083:1152	RESULTS Analyses using a single weekly timepoint of 97 368 total products in March 2018 identified 2699 ready meals and pizzas, and showed that lower price ready meals had significantly lower levels of fat, saturates, sugar and salt (p<0.001).
31253615	5	23	theme	websites	839:846	arg1	webpages					811:818	the webpages	807:818	the webpages of the supermarket websites	807:846	METHODS Weekly extraction of nutrition and availability data of products was extracted from the webpages of the supermarket websites.
31253615	7	24	theme	lower	1055:1059	arg1	meals					1073:1077	lower price ready meals	1055:1077	lower price ready meals	1055:1077	RESULTS Analyses using a single weekly timepoint of 97 368 total products in March 2018 identified 2699 ready meals and pizzas, and showed that lower price ready meals had significantly lower levels of fat, saturates, sugar and salt (p<0.001).
31253615	4	25	theme	multiple	694:701	arg1	timepoints					703:712	multiple timepoints	694:712	multiple timepoints	694:712	As of June 2018, foodDB has 3 193 171 observations of 128 283 distinct food and drink products measured at multiple timepoints.
31253615	1	26	theme	composition	164:174	arg1	tables					176:181	food composition tables	159:181	food composition tables	159:181	OBJECTIVES Traditional methods for creating food composition tables struggle to cope with the large number of products and the rapid pace of change in the food and drink marketplace.
31253615	0	27	theme	composition	9:19	arg1	databases					21:29	Nutrient composition databases	0:29	Nutrient composition databases in the age of big data: foodDB, a comprehensive, real-time database infrastructure.	0:113	Nutrient composition databases in the age of big data: foodDB, a comprehensive, real-time database infrastructure.
31253615	7	28	theme	ready	1015:1019	arg1	meals					1021:1025	2699 ready meals	1010:1025	2699 ready meals	1010:1025	RESULTS Analyses using a single weekly timepoint of 97 368 total products in March 2018 identified 2699 ready meals and pizzas, and showed that lower price ready meals had significantly lower levels of fat, saturates, sugar and salt (p<0.001).
31253615	7	29	contain	had	1079:1081	arg2	levels					1103:1108	significantly lower levels	1083:1108	significantly lower levels of fat, saturates, sugar and salt (p<0.001)	1083:1152	RESULTS Analyses using a single weekly timepoint of 97 368 total products in March 2018 identified 2699 ready meals and pizzas, and showed that lower price ready meals had significantly lower levels of fat, saturates, sugar and salt (p<0.001).
31253615	7	29	contain	had	1079:1081	arg1	meals					1073:1077	lower price ready meals	1055:1077	lower price ready meals	1055:1077	RESULTS Analyses using a single weekly timepoint of 97 368 total products in March 2018 identified 2699 ready meals and pizzas, and showed that lower price ready meals had significantly lower levels of fat, saturates, sugar and salt (p<0.001).
31253615	9	30	theme	collection	1457:1466	arg1	granularity					1442:1452	granularity	1442:1452	granularity	1442:1452	CONCLUSIONS foodDB is a powerful new tool for monitoring the food and drink marketplace, the comprehensive sampling and granularity of collection provides power for revealing analyses of the relationship between nutritional quality and marketing of branded foods, timely observation of product reformulation and other changes to the food marketplace.
31253615	9	30	theme	collection	1457:1466	arg1	sampling					1429:1436	comprehensive sampling	1415:1436	comprehensive sampling	1415:1436	CONCLUSIONS foodDB is a powerful new tool for monitoring the food and drink marketplace, the comprehensive sampling and granularity of collection provides power for revealing analyses of the relationship between nutritional quality and marketing of branded foods, timely observation of product reformulation and other changes to the food marketplace.
31253615	9	31	theme	timely	1586:1591	arg1	observation					1593:1603	timely observation	1586:1603	timely observation of product reformulation	1586:1628	CONCLUSIONS foodDB is a powerful new tool for monitoring the food and drink marketplace, the comprehensive sampling and granularity of collection provides power for revealing analyses of the relationship between nutritional quality and marketing of branded foods, timely observation of product reformulation and other changes to the food marketplace.
31253615	0	32	theme	Nutrient	0:7	arg1	databases					21:29	Nutrient composition databases	0:29	Nutrient composition databases in the age of big data: foodDB, a comprehensive, real-time database infrastructure.	0:113	Nutrient composition databases in the age of big data: foodDB, a comprehensive, real-time database infrastructure.
31253615	1	33	from	change	256:261	arg1	marketplace					285:295	the food and drink marketplace	266:295	marketplace	285:295	OBJECTIVES Traditional methods for creating food composition tables struggle to cope with the large number of products and the rapid pace of change in the food and drink marketplace.
31253615	3	34	theme	available	519:527	arg1	data					483:486	data	483:486	data weekly on all foods and drinks available on six major UK supermarket websites since November 2017	483:584	DESIGN foodDB has been used to collect data weekly on all foods and drinks available on six major UK supermarket websites since November 2017.
31253615	7	35	dep	RESULTS	911:917	arg1	identified					999:1008	identified	999:1008	identified 2699 ready meals and pizzas	999:1036	RESULTS Analyses using a single weekly timepoint of 97 368 total products in March 2018 identified 2699 ready meals and pizzas, and showed that lower price ready meals had significantly lower levels of fat, saturates, sugar and salt (p<0.001).
31253615	7	35	dep	RESULTS	911:917	arg1	showed					1043:1048	showed	1043:1048	showed that lower price ready meals had significantly lower levels of fat, saturates, sugar and salt (p<0.001)	1043:1152	RESULTS Analyses using a single weekly timepoint of 97 368 total products in March 2018 identified 2699 ready meals and pizzas, and showed that lower price ready meals had significantly lower levels of fat, saturates, sugar and salt (p<0.001).
31253615	2	36	theme	data	334:337	arg1	approach					339:346	a big data approach	328:346	a big data approach to the analysis of this marketplace	328:382	This paper introduces foodDB, a big data approach to the analysis of this marketplace, and presents analyses illustrating its research potential.
31253615	2	36	theme	data	334:337	arg1	foodDB					320:325	foodDB	320:325	foodDB	320:325	This paper introduces foodDB, a big data approach to the analysis of this marketplace, and presents analyses illustrating its research potential.
31253615	1	37	theme	change	256:261	arg1	number					215:220	the large number	205:220	the large number of products	205:232	OBJECTIVES Traditional methods for creating food composition tables struggle to cope with the large number of products and the rapid pace of change in the food and drink marketplace.
31253615	1	37	theme	change	256:261	arg1	pace					248:251	the rapid pace	238:251	the rapid pace of change in the food and drink marketplace	238:295	OBJECTIVES Traditional methods for creating food composition tables struggle to cope with the large number of products and the rapid pace of change in the food and drink marketplace.
31253615	4	38	theme	products	673:680	arg1	observations					625:636	3 193 171 observations	615:636	3 193 171 observations of 128 283 distinct food and drink products measured at multiple timepoints	615:712	As of June 2018, foodDB has 3 193 171 observations of 128 283 distinct food and drink products measured at multiple timepoints.
31253615	2	39	theme	marketplace	372:382	arg1	analysis					355:362	the analysis	351:362	the analysis of this marketplace	351:382	This paper introduces foodDB, a big data approach to the analysis of this marketplace, and presents analyses illustrating its research potential.
31253615	4	40	theme	food	658:661	arg1	products					673:680	128 283 distinct food and drink products	641:680	128 283 distinct food and drink products measured at multiple timepoints	641:712	As of June 2018, foodDB has 3 193 171 observations of 128 283 distinct food and drink products measured at multiple timepoints.
31253615	0	41	theme	database	90:97	arg1	foodDB					55:60	foodDB	55:60	foodDB	55:60	Nutrient composition databases in the age of big data: foodDB, a comprehensive, real-time database infrastructure.
31253615	0	41	theme	database	90:97	arg1	infrastructure					99:112	a comprehensive, real-time database infrastructure	63:112	a comprehensive, real-time database infrastructure	63:112	Nutrient composition databases in the age of big data: foodDB, a comprehensive, real-time database infrastructure.
31253615	4	42	theme	drink	667:671	arg1	products					673:680	128 283 distinct food and drink products	641:680	128 283 distinct food and drink products measured at multiple timepoints	641:712	As of June 2018, foodDB has 3 193 171 observations of 128 283 distinct food and drink products measured at multiple timepoints.
31253615	9	43	theme	CONCLUSIONS	1322:1332	arg1	tool					1359:1362	a powerful new tool	1344:1362	a powerful new tool for monitoring the food and drink marketplace	1344:1408	CONCLUSIONS foodDB is a powerful new tool for monitoring the food and drink marketplace, the comprehensive sampling and granularity of collection provides power for revealing analyses of the relationship between nutritional quality and marketing of branded foods, timely observation of product reformulation and other changes to the food marketplace.
31253615	9	43	theme	CONCLUSIONS	1322:1332	arg1	foodDB					1334:1339	CONCLUSIONS foodDB	1322:1339	CONCLUSIONS foodDB	1322:1339	CONCLUSIONS foodDB is a powerful new tool for monitoring the food and drink marketplace, the comprehensive sampling and granularity of collection provides power for revealing analyses of the relationship between nutritional quality and marketing of branded foods, timely observation of product reformulation and other changes to the food marketplace.
31253615	2	44	theme	big	330:332	arg1	approach					339:346	a big data approach	328:346	a big data approach to the analysis of this marketplace	328:382	This paper introduces foodDB, a big data approach to the analysis of this marketplace, and presents analyses illustrating its research potential.
31253615	2	44	theme	big	330:332	arg1	foodDB					320:325	foodDB	320:325	foodDB	320:325	This paper introduces foodDB, a big data approach to the analysis of this marketplace, and presents analyses illustrating its research potential.
31253615	7	45	theme	ready	1067:1071	arg1	meals					1073:1077	lower price ready meals	1055:1077	lower price ready meals	1055:1077	RESULTS Analyses using a single weekly timepoint of 97 368 total products in March 2018 identified 2699 ready meals and pizzas, and showed that lower price ready meals had significantly lower levels of fat, saturates, sugar and salt (p<0.001).
31253615	9	46	theme	food	1383:1386	arg1	marketplace					1398:1408	the food and drink marketplace	1379:1408	marketplace	1398:1408	CONCLUSIONS foodDB is a powerful new tool for monitoring the food and drink marketplace, the comprehensive sampling and granularity of collection provides power for revealing analyses of the relationship between nutritional quality and marketing of branded foods, timely observation of product reformulation and other changes to the food marketplace.
31253615	9	47	theme	reformulation	1616:1628	arg1	changes					1640:1646	other changes	1634:1646	other changes to the food marketplace	1634:1670	CONCLUSIONS foodDB is a powerful new tool for monitoring the food and drink marketplace, the comprehensive sampling and granularity of collection provides power for revealing analyses of the relationship between nutritional quality and marketing of branded foods, timely observation of product reformulation and other changes to the food marketplace.
31253615	9	47	theme	reformulation	1616:1628	arg1	analyses					1497:1504	analyses	1497:1504	analyses of the relationship between nutritional quality and marketing of branded foods	1497:1583	CONCLUSIONS foodDB is a powerful new tool for monitoring the food and drink marketplace, the comprehensive sampling and granularity of collection provides power for revealing analyses of the relationship between nutritional quality and marketing of branded foods, timely observation of product reformulation and other changes to the food marketplace.
31253615	9	47	theme	reformulation	1616:1628	arg1	observation					1593:1603	timely observation	1586:1603	timely observation of product reformulation	1586:1628	CONCLUSIONS foodDB is a powerful new tool for monitoring the food and drink marketplace, the comprehensive sampling and granularity of collection provides power for revealing analyses of the relationship between nutritional quality and marketing of branded foods, timely observation of product reformulation and other changes to the food marketplace.
31253615	7	48	from	timepoint	950:958	arg1	March					988:992	March	988:992	March	988:992	RESULTS Analyses using a single weekly timepoint of 97 368 total products in March 2018 identified 2699 ready meals and pizzas, and showed that lower price ready meals had significantly lower levels of fat, saturates, sugar and salt (p<0.001).
31253615	3	49	theme	major	536:540	arg1	websites					557:564	six major UK supermarket websites	532:564	six major UK supermarket websites since November 2017	532:584	DESIGN foodDB has been used to collect data weekly on all foods and drinks available on six major UK supermarket websites since November 2017.
31253615	9	50	theme	powerful	1346:1353	arg1	tool					1359:1362	a powerful new tool	1344:1362	a powerful new tool for monitoring the food and drink marketplace	1344:1408	CONCLUSIONS foodDB is a powerful new tool for monitoring the food and drink marketplace, the comprehensive sampling and granularity of collection provides power for revealing analyses of the relationship between nutritional quality and marketing of branded foods, timely observation of product reformulation and other changes to the food marketplace.
31253615	9	50	theme	powerful	1346:1353	arg1	foodDB					1334:1339	CONCLUSIONS foodDB	1322:1339	CONCLUSIONS foodDB	1322:1339	CONCLUSIONS foodDB is a powerful new tool for monitoring the food and drink marketplace, the comprehensive sampling and granularity of collection provides power for revealing analyses of the relationship between nutritional quality and marketing of branded foods, timely observation of product reformulation and other changes to the food marketplace.
31253615	5	51	theme	products	779:786	arg1	extraction					730:739	METHODS Weekly extraction	715:739	METHODS Weekly extraction of nutrition and availability data of products	715:786	METHODS Weekly extraction of nutrition and availability data of products was extracted from the webpages of the supermarket websites.
31253615	2	52	theme	research	424:431	arg1	potential					433:441	its research potential	420:441	its research potential	420:441	This paper introduces foodDB, a big data approach to the analysis of this marketplace, and presents analyses illustrating its research potential.
31253615	1	53	theme	food	270:273	arg1	marketplace					285:295	the food and drink marketplace	266:295	marketplace	285:295	OBJECTIVES Traditional methods for creating food composition tables struggle to cope with the large number of products and the rapid pace of change in the food and drink marketplace.
31253615	5	54	theme	METHODS	715:721	arg1	extraction					730:739	METHODS Weekly extraction	715:739	METHODS Weekly extraction of nutrition and availability data of products	715:786	METHODS Weekly extraction of nutrition and availability data of products was extracted from the webpages of the supermarket websites.
31253615	3	55	theme	UK	542:543	arg1	websites					557:564	six major UK supermarket websites	532:564	six major UK supermarket websites since November 2017	532:584	DESIGN foodDB has been used to collect data weekly on all foods and drinks available on six major UK supermarket websites since November 2017.
31253615	0	56	theme	big	45:47	arg1	data					49:52	big data	45:52	big data	45:52	Nutrient composition databases in the age of big data: foodDB, a comprehensive, real-time database infrastructure.
31253615	7	57	theme	products	976:983	arg1	timepoint					950:958	a single weekly timepoint	934:958	a single weekly timepoint of 97 368 total products in March 2018	934:997	RESULTS Analyses using a single weekly timepoint of 97 368 total products in March 2018 identified 2699 ready meals and pizzas, and showed that lower price ready meals had significantly lower levels of fat, saturates, sugar and salt (p<0.001).
31253615	8	58	theme	new	1302:1304	arg1	entries					1313:1319	new market entries	1302:1319	new market entries	1302:1319	Longitudinal analyses of 903 pizzas revealed that 10.8% changed their nutritional formulation over 6 months, and 29.9% were either discontinued or new market entries.
31253615	9	59	theme	comprehensive	1415:1427	arg1	sampling					1429:1436	comprehensive sampling	1415:1436	comprehensive sampling	1415:1436	CONCLUSIONS foodDB is a powerful new tool for monitoring the food and drink marketplace, the comprehensive sampling and granularity of collection provides power for revealing analyses of the relationship between nutritional quality and marketing of branded foods, timely observation of product reformulation and other changes to the food marketplace.
31253615	7	60	theme	price	1061:1065	arg1	meals					1073:1077	lower price ready meals	1055:1077	lower price ready meals	1055:1077	RESULTS Analyses using a single weekly timepoint of 97 368 total products in March 2018 identified 2699 ready meals and pizzas, and showed that lower price ready meals had significantly lower levels of fat, saturates, sugar and salt (p<0.001).
31253615	1	61	theme	drink	279:283	arg1	marketplace					285:295	the food and drink marketplace	266:295	marketplace	285:295	OBJECTIVES Traditional methods for creating food composition tables struggle to cope with the large number of products and the rapid pace of change in the food and drink marketplace.
31253615	9	62	theme	nutritional	1534:1544	arg1	quality					1546:1552	nutritional quality	1534:1552	nutritional quality	1534:1552	CONCLUSIONS foodDB is a powerful new tool for monitoring the food and drink marketplace, the comprehensive sampling and granularity of collection provides power for revealing analyses of the relationship between nutritional quality and marketing of branded foods, timely observation of product reformulation and other changes to the food marketplace.
31253615	7	63	theme	single	936:941	arg1	timepoint					950:958	a single weekly timepoint	934:958	a single weekly timepoint of 97 368 total products in March 2018	934:997	RESULTS Analyses using a single weekly timepoint of 97 368 total products in March 2018 identified 2699 ready meals and pizzas, and showed that lower price ready meals had significantly lower levels of fat, saturates, sugar and salt (p<0.001).
31253615	8	64	theme	pizzas	1184:1189	arg1	analyses					1168:1175	Longitudinal analyses	1155:1175	Longitudinal analyses of 903 pizzas	1155:1189	Longitudinal analyses of 903 pizzas revealed that 10.8% changed their nutritional formulation over 6 months, and 29.9% were either discontinued or new market entries.
31253615	9	65	theme	relationship	1513:1524	arg1	changes					1640:1646	other changes	1634:1646	other changes to the food marketplace	1634:1670	CONCLUSIONS foodDB is a powerful new tool for monitoring the food and drink marketplace, the comprehensive sampling and granularity of collection provides power for revealing analyses of the relationship between nutritional quality and marketing of branded foods, timely observation of product reformulation and other changes to the food marketplace.
31253615	9	65	theme	relationship	1513:1524	arg1	analyses					1497:1504	analyses	1497:1504	analyses of the relationship between nutritional quality and marketing of branded foods	1497:1583	CONCLUSIONS foodDB is a powerful new tool for monitoring the food and drink marketplace, the comprehensive sampling and granularity of collection provides power for revealing analyses of the relationship between nutritional quality and marketing of branded foods, timely observation of product reformulation and other changes to the food marketplace.
31253615	9	65	theme	relationship	1513:1524	arg1	observation					1593:1603	timely observation	1586:1603	timely observation of product reformulation	1586:1628	CONCLUSIONS foodDB is a powerful new tool for monitoring the food and drink marketplace, the comprehensive sampling and granularity of collection provides power for revealing analyses of the relationship between nutritional quality and marketing of branded foods, timely observation of product reformulation and other changes to the food marketplace.
31253615	3	66	used	used	467:470	arg2	foodDB					451:456	DESIGN foodDB	444:456	DESIGN foodDB	444:456	DESIGN foodDB has been used to collect data weekly on all foods and drinks available on six major UK supermarket websites since November 2017.
31253615	7	67	theme	weekly	943:948	arg1	timepoint					950:958	a single weekly timepoint	934:958	a single weekly timepoint of 97 368 total products in March 2018	934:997	RESULTS Analyses using a single weekly timepoint of 97 368 total products in March 2018 identified 2699 ready meals and pizzas, and showed that lower price ready meals had significantly lower levels of fat, saturates, sugar and salt (p<0.001).
31253615	4	68	theme	distinct	649:656	arg1	products					673:680	128 283 distinct food and drink products	641:680	128 283 distinct food and drink products measured at multiple timepoints	641:712	As of June 2018, foodDB has 3 193 171 observations of 128 283 distinct food and drink products measured at multiple timepoints.
31253615	1	69	theme	large	209:213	arg1	number					215:220	the large number	205:220	the large number of products	205:232	OBJECTIVES Traditional methods for creating food composition tables struggle to cope with the large number of products and the rapid pace of change in the food and drink marketplace.
31253615	1	70	from	number	215:220	arg1	marketplace					285:295	the food and drink marketplace	266:295	marketplace	285:295	OBJECTIVES Traditional methods for creating food composition tables struggle to cope with the large number of products and the rapid pace of change in the food and drink marketplace.
31253615	0	71	dep	databases	21:29	arg1	foodDB					55:60	foodDB	55:60	foodDB	55:60	Nutrient composition databases in the age of big data: foodDB, a comprehensive, real-time database infrastructure.
31253615	0	71	dep	databases	21:29	arg1	infrastructure					99:112	a comprehensive, real-time database infrastructure	63:112	a comprehensive, real-time database infrastructure	63:112	Nutrient composition databases in the age of big data: foodDB, a comprehensive, real-time database infrastructure.
31253615	3	72	theme	DESIGN	444:449	arg1	foodDB					451:456	DESIGN foodDB	444:456	DESIGN foodDB	444:456	DESIGN foodDB has been used to collect data weekly on all foods and drinks available on six major UK supermarket websites since November 2017.
31253615	9	73	dep	sampling	1429:1436	arg1	the					1411:1413	the	1411:1413	the	1411:1413	CONCLUSIONS foodDB is a powerful new tool for monitoring the food and drink marketplace, the comprehensive sampling and granularity of collection provides power for revealing analyses of the relationship between nutritional quality and marketing of branded foods, timely observation of product reformulation and other changes to the food marketplace.
31253615	9	74	theme	food	1655:1658	arg1	marketplace					1660:1670	the food marketplace	1651:1670	the food marketplace	1651:1670	CONCLUSIONS foodDB is a powerful new tool for monitoring the food and drink marketplace, the comprehensive sampling and granularity of collection provides power for revealing analyses of the relationship between nutritional quality and marketing of branded foods, timely observation of product reformulation and other changes to the food marketplace.
31253615	3	75	theme	supermarket	545:555	arg1	websites					557:564	six major UK supermarket websites	532:564	six major UK supermarket websites since November 2017	532:584	DESIGN foodDB has been used to collect data weekly on all foods and drinks available on six major UK supermarket websites since November 2017.
31253615	3	76	dep	foods	502:506	arg1	all					498:500	all	498:500	all	498:500	DESIGN foodDB has been used to collect data weekly on all foods and drinks available on six major UK supermarket websites since November 2017.
31253615	1	77	theme	Traditional	126:136	arg1	methods					138:144	Traditional methods	126:144	Traditional methods	126:144	OBJECTIVES Traditional methods for creating food composition tables struggle to cope with the large number of products and the rapid pace of change in the food and drink marketplace.
31253615	0	78	theme	data	49:52	arg1	age					38:40	the age	34:40	the age of big data	34:52	Nutrient composition databases in the age of big data: foodDB, a comprehensive, real-time database infrastructure.
31253615	5	79	theme	availability	758:769	arg1	extraction					730:739	METHODS Weekly extraction	715:739	METHODS Weekly extraction of nutrition and availability data of products	715:786	METHODS Weekly extraction of nutrition and availability data of products was extracted from the webpages of the supermarket websites.
31253615	7	80	theme	lower	1097:1101	arg1	levels					1103:1108	significantly lower levels	1083:1108	significantly lower levels of fat, saturates, sugar and salt (p<0.001)	1083:1152	RESULTS Analyses using a single weekly timepoint of 97 368 total products in March 2018 identified 2699 ready meals and pizzas, and showed that lower price ready meals had significantly lower levels of fat, saturates, sugar and salt (p<0.001).
31253615	9	81	theme	product	1608:1614	arg1	reformulation					1616:1628	product reformulation	1608:1628	product reformulation	1608:1628	CONCLUSIONS foodDB is a powerful new tool for monitoring the food and drink marketplace, the comprehensive sampling and granularity of collection provides power for revealing analyses of the relationship between nutritional quality and marketing of branded foods, timely observation of product reformulation and other changes to the food marketplace.
31253615	1	82	theme	products	225:232	arg1	number					215:220	the large number	205:220	the large number of products	205:232	OBJECTIVES Traditional methods for creating food composition tables struggle to cope with the large number of products and the rapid pace of change in the food and drink marketplace.
31253615	1	82	theme	products	225:232	arg1	pace					248:251	the rapid pace	238:251	the rapid pace of change in the food and drink marketplace	238:295	OBJECTIVES Traditional methods for creating food composition tables struggle to cope with the large number of products and the rapid pace of change in the food and drink marketplace.
30016228	3	0	theme	Cohnella	305:312	arg1	members					284:290	members	284:290	members of the genus Cohnella	284:312	The permafrost isolate clustered with members of the genus Cohnella on the basis of 16S rRNA gene sequence analysis and showed the highest sequence similarity to Cohnella saccharovorans CJ22T (96.3 %), followed by Cohnella cellulosilytica FCN3-3T (96.0 %) and Cohnella panacarvi KCTC 13060T (96.0 %).
30016228	2	1	theme	rod-shaped	107:116	arg1	bacterium					152:160	A facultative anaerobic, rod-shaped, endospore-forming and non-motile bacterium	82:160	A facultative anaerobic, rod-shaped, endospore-forming and non-motile bacterium	82:160	A facultative anaerobic, rod-shaped, endospore-forming and non-motile bacterium was isolated from permafrost sediment cores in the Kolyma lowland, Siberia, Russia.
30016228	7	2	theme	Cohnella	1056:1063	arg1	species					1065:1071	other Cohnella species	1050:1071	other Cohnella species	1050:1071	The estimated genome size of strain 20.16T is 4.34 Mb (lower than those in other Cohnella species).
30016228	7	3	theme	genome	989:994	arg1	size					996:999	The estimated genome size	975:999	The estimated genome size of strain 20.16T	975:1016	The estimated genome size of strain 20.16T is 4.34 Mb (lower than those in other Cohnella species).
30016228	7	3	theme	genome	989:994	arg1	4.34 Mb					1021:1027	4.34 Mb	1021:1027	4.34 Mb (lower than those in other Cohnella species)	1021:1072	The estimated genome size of strain 20.16T is 4.34 Mb (lower than those in other Cohnella species).
30016228	9	4	theme	physiological	1221:1233	arg1	characterization					1251:1266	chemotaxonomic, physiological and biochemical characterization	1205:1266	chemotaxonomic, physiological and biochemical characterization	1205:1266	The results of chemotaxonomic, physiological and biochemical characterization allowed clear differentiation of strain 20.16T from the closest Cohnella species.
30016228	6	5	theme	peptydoglycan	951:963	arg1	A1γ					970:972	the peptydoglycan type A1γ	947:972	the peptydoglycan type A1γ	947:972	The molar ratio and composition of major amino acids (meso-diaminopimelic acid, alanine, and glutamic acid) correspond to the peptydoglycan type A1γ.
30016228	7	6	dep	4.34 Mb	1021:1027	arg1	lower					1030:1034	lower	1030:1034	lower	1030:1034	The estimated genome size of strain 20.16T is 4.34 Mb (lower than those in other Cohnella species).
30016228	6	7	theme	acids	872:876	arg1	composition					845:855	composition	845:855	composition	845:855	The molar ratio and composition of major amino acids (meso-diaminopimelic acid, alanine, and glutamic acid) correspond to the peptydoglycan type A1γ.
30016228	6	7	theme	acids	872:876	arg1	ratio					835:839	The molar ratio	825:839	The molar ratio	825:839	The molar ratio and composition of major amino acids (meso-diaminopimelic acid, alanine, and glutamic acid) correspond to the peptydoglycan type A1γ.
30016228	3	8	dep	analysis	353:360	arg1	the					317:319	the	317:319	the	317:319	The permafrost isolate clustered with members of the genus Cohnella on the basis of 16S rRNA gene sequence analysis and showed the highest sequence similarity to Cohnella saccharovorans CJ22T (96.3 %), followed by Cohnella cellulosilytica FCN3-3T (96.0 %) and Cohnella panacarvi KCTC 13060T (96.0 %).
30016228	3	8	dep	analysis	353:360	arg1	basis					321:325	basis	321:325	basis	321:325	The permafrost isolate clustered with members of the genus Cohnella on the basis of 16S rRNA gene sequence analysis and showed the highest sequence similarity to Cohnella saccharovorans CJ22T (96.3 %), followed by Cohnella cellulosilytica FCN3-3T (96.0 %) and Cohnella panacarvi KCTC 13060T (96.0 %).
30016228	7	9	theme	other	1050:1054	arg1	species					1065:1071	other Cohnella species	1050:1071	other Cohnella species	1050:1071	The estimated genome size of strain 20.16T is 4.34 Mb (lower than those in other Cohnella species).
30016228	10	10	theme	new	1376:1378	arg1	species					1380:1386	a new species	1374:1386	a new species	1374:1386	Based on data provided, a new species Cohnella kolymensis sp.
30016228	2	11	from	cores	200:204	arg1	Russia					238:243	Russia	238:243	Russia	238:243	A facultative anaerobic, rod-shaped, endospore-forming and non-motile bacterium was isolated from permafrost sediment cores in the Kolyma lowland, Siberia, Russia.
30016228	2	11	from	cores	200:204	arg1	Siberia					229:235	Siberia	229:235	Siberia	229:235	A facultative anaerobic, rod-shaped, endospore-forming and non-motile bacterium was isolated from permafrost sediment cores in the Kolyma lowland, Siberia, Russia.
30016228	2	11	from	cores	200:204	arg1	lowland					220:226	lowland	220:226	lowland	220:226	A facultative anaerobic, rod-shaped, endospore-forming and non-motile bacterium was isolated from permafrost sediment cores in the Kolyma lowland, Siberia, Russia.
30016228	2	12	theme	anaerobic	96:104	arg1	bacterium					152:160	A facultative anaerobic, rod-shaped, endospore-forming and non-motile bacterium	82:160	A facultative anaerobic, rod-shaped, endospore-forming and non-motile bacterium	82:160	A facultative anaerobic, rod-shaped, endospore-forming and non-motile bacterium was isolated from permafrost sediment cores in the Kolyma lowland, Siberia, Russia.
30016228	4	13	theme	Cohnella	704:711	arg1	members					683:689	members	683:689	members of the genus Cohnella	683:711	The chemotaxonomic characteristics (quinone system, cellular fatty acids and polar lipid profile) of strain 20.16T were consistent with members of the genus Cohnella.
30016228	3	14	theme	gene	339:342	arg1	analysis					353:360	16S rRNA gene sequence analysis	330:360	16S rRNA gene sequence analysis	330:360	The permafrost isolate clustered with members of the genus Cohnella on the basis of 16S rRNA gene sequence analysis and showed the highest sequence similarity to Cohnella saccharovorans CJ22T (96.3 %), followed by Cohnella cellulosilytica FCN3-3T (96.0 %) and Cohnella panacarvi KCTC 13060T (96.0 %).
30016228	6	15	theme	major	860:864	arg1	alanine					905:911	alanine	905:911	alanine	905:911	The molar ratio and composition of major amino acids (meso-diaminopimelic acid, alanine, and glutamic acid) correspond to the peptydoglycan type A1γ.
30016228	6	15	theme	major	860:864	arg1	acids					872:876	major amino acids	860:876	major amino acids (meso-diaminopimelic acid, alanine, and glutamic acid)	860:931	The molar ratio and composition of major amino acids (meso-diaminopimelic acid, alanine, and glutamic acid) correspond to the peptydoglycan type A1γ.
30016228	6	15	theme	major	860:864	arg1	acid					899:902	meso-diaminopimelic acid	879:902	meso-diaminopimelic acid	879:902	The molar ratio and composition of major amino acids (meso-diaminopimelic acid, alanine, and glutamic acid) correspond to the peptydoglycan type A1γ.
30016228	6	15	theme	major	860:864	arg1	acid					927:930	glutamic acid	918:930	glutamic acid	918:930	The molar ratio and composition of major amino acids (meso-diaminopimelic acid, alanine, and glutamic acid) correspond to the peptydoglycan type A1γ.
30016228	3	16	dep	showed	366:371	arg1	%					444:444	96.3 %	439:444	96.3 %	439:444	The permafrost isolate clustered with members of the genus Cohnella on the basis of 16S rRNA gene sequence analysis and showed the highest sequence similarity to Cohnella saccharovorans CJ22T (96.3 %), followed by Cohnella cellulosilytica FCN3-3T (96.0 %) and Cohnella panacarvi KCTC 13060T (96.0 %).
30016228	11	17	theme	B-2846T=DSM	1448:1458	arg1	20.16T					1435:1440	20.16T	1435:1440	20.16T (=VKM B-2846T=DSM 104983T) as the type strain	1435:1486	nov. is proposed, with 20.16T (=VKM B-2846T=DSM 104983T) as the type strain.
30016228	11	17	theme	B-2846T=DSM	1448:1458	arg1	104983T					1460:1466	=VKM B-2846T=DSM 104983T	1443:1466	=VKM B-2846T=DSM 104983T	1443:1466	nov. is proposed, with 20.16T (=VKM B-2846T=DSM 104983T) as the type strain.
30016228	3	18	theme	Cohnella	506:513	arg1	13060T					530:535	Cohnella panacarvi KCTC 13060T	506:535	Cohnella panacarvi KCTC 13060T (96.0 %)	506:544	The permafrost isolate clustered with members of the genus Cohnella on the basis of 16S rRNA gene sequence analysis and showed the highest sequence similarity to Cohnella saccharovorans CJ22T (96.3 %), followed by Cohnella cellulosilytica FCN3-3T (96.0 %) and Cohnella panacarvi KCTC 13060T (96.0 %).
30016228	3	18	theme	Cohnella	506:513	arg1	%					543:543	96.0 %	538:543	96.0 %	538:543	The permafrost isolate clustered with members of the genus Cohnella on the basis of 16S rRNA gene sequence analysis and showed the highest sequence similarity to Cohnella saccharovorans CJ22T (96.3 %), followed by Cohnella cellulosilytica FCN3-3T (96.0 %) and Cohnella panacarvi KCTC 13060T (96.0 %).
30016228	6	19	dep	acids	872:876	arg1	alanine					905:911	alanine	905:911	alanine	905:911	The molar ratio and composition of major amino acids (meso-diaminopimelic acid, alanine, and glutamic acid) correspond to the peptydoglycan type A1γ.
30016228	6	19	dep	acids	872:876	arg1	acids					872:876	major amino acids	860:876	major amino acids (meso-diaminopimelic acid, alanine, and glutamic acid)	860:931	The molar ratio and composition of major amino acids (meso-diaminopimelic acid, alanine, and glutamic acid) correspond to the peptydoglycan type A1γ.
30016228	6	19	dep	acids	872:876	arg1	acid					899:902	meso-diaminopimelic acid	879:902	meso-diaminopimelic acid	879:902	The molar ratio and composition of major amino acids (meso-diaminopimelic acid, alanine, and glutamic acid) correspond to the peptydoglycan type A1γ.
30016228	6	19	dep	acids	872:876	arg1	acid					927:930	glutamic acid	918:930	glutamic acid	918:930	The molar ratio and composition of major amino acids (meso-diaminopimelic acid, alanine, and glutamic acid) correspond to the peptydoglycan type A1γ.
30016228	8	20	theme	%	1115:1115	arg1	G+C content					1092:1102	a G+C content	1090:1102	a G+C content of 50.5 mol%	1090:1115	The genome has a G+C content of 50.5 mol% and encodes 4843 predicted genes, of these 4740 are protein-coding ones.
30016228	9	21	theme	20.16T	1308:1313	arg1	differentiation					1282:1296	clear differentiation	1276:1296	clear differentiation of strain 20.16T from the closest Cohnella species	1276:1347	The results of chemotaxonomic, physiological and biochemical characterization allowed clear differentiation of strain 20.16T from the closest Cohnella species.
30016228	2	22	theme	non-motile	141:150	arg1	bacterium					152:160	A facultative anaerobic, rod-shaped, endospore-forming and non-motile bacterium	82:160	A facultative anaerobic, rod-shaped, endospore-forming and non-motile bacterium	82:160	A facultative anaerobic, rod-shaped, endospore-forming and non-motile bacterium was isolated from permafrost sediment cores in the Kolyma lowland, Siberia, Russia.
30016228	8	23	theme	protein-coding	1169:1182	arg1	4740					1160:1163	4740	1160:1163	4740	1160:1163	The genome has a G+C content of 50.5 mol% and encodes 4843 predicted genes, of these 4740 are protein-coding ones.
30016228	8	23	theme	protein-coding	1169:1182	arg1	ones					1184:1187	protein-coding ones	1169:1187	protein-coding ones	1169:1187	The genome has a G+C content of 50.5 mol% and encodes 4843 predicted genes, of these 4740 are protein-coding ones.
30016228	8	24	contain	has	1086:1088	arg2	G+C content					1092:1102	a G+C content	1090:1102	a G+C content of 50.5 mol%	1090:1115	The genome has a G+C content of 50.5 mol% and encodes 4843 predicted genes, of these 4740 are protein-coding ones.
30016228	8	24	contain	has	1086:1088	arg1	genome					1079:1084	The genome	1075:1084	The genome	1075:1084	The genome has a G+C content of 50.5 mol% and encodes 4843 predicted genes, of these 4740 are protein-coding ones.
30016228	8	24	contain	has	1086:1088	arg1	genes					1144:1148	4843 predicted genes	1129:1148	4843 predicted genes	1129:1148	The genome has a G+C content of 50.5 mol% and encodes 4843 predicted genes, of these 4740 are protein-coding ones.
30016228	3	25	theme	16S	330:332	arg1	analysis					353:360	16S rRNA gene sequence analysis	330:360	16S rRNA gene sequence analysis	330:360	The permafrost isolate clustered with members of the genus Cohnella on the basis of 16S rRNA gene sequence analysis and showed the highest sequence similarity to Cohnella saccharovorans CJ22T (96.3 %), followed by Cohnella cellulosilytica FCN3-3T (96.0 %) and Cohnella panacarvi KCTC 13060T (96.0 %).
30016228	9	26	theme	biochemical	1239:1249	arg1	characterization					1251:1266	chemotaxonomic, physiological and biochemical characterization	1205:1266	chemotaxonomic, physiological and biochemical characterization	1205:1266	The results of chemotaxonomic, physiological and biochemical characterization allowed clear differentiation of strain 20.16T from the closest Cohnella species.
30016228	5	27	theme	peptidoglycan	718:730	arg1	diaminoacids					732:743	The peptidoglycan diaminoacids	714:743	The peptidoglycan diaminoacids	714:743	The peptidoglycan diaminoacids included meso-diaminopimelic acid and a small amount of ll-diaminopimelic acid.
30016228	1	28	theme	Siberian	61:68	arg1	permafrost					70:79	Siberian permafrost	61:79	Siberian permafrost	61:79	nov., a novel bacillus isolated from Siberian permafrost.
30016228	4	29	theme	fatty	608:612	arg1	acids					614:618	cellular fatty acids	599:618	cellular fatty acids	599:618	The chemotaxonomic characteristics (quinone system, cellular fatty acids and polar lipid profile) of strain 20.16T were consistent with members of the genus Cohnella.
30016228	0	30	theme	kolymensis	9:18	arg1	sp					20:21	Cohnella kolymensis sp	0:21	Cohnella kolymensis sp.	0:22	Cohnella kolymensis sp.
30016228	2	31	theme	endospore-forming	119:135	arg1	bacterium					152:160	A facultative anaerobic, rod-shaped, endospore-forming and non-motile bacterium	82:160	A facultative anaerobic, rod-shaped, endospore-forming and non-motile bacterium	82:160	A facultative anaerobic, rod-shaped, endospore-forming and non-motile bacterium was isolated from permafrost sediment cores in the Kolyma lowland, Siberia, Russia.
30016228	10	32	theme	kolymensis	1397:1406	arg1	sp					1408:1409	Cohnella kolymensis sp	1388:1409	Cohnella kolymensis sp	1388:1409	Based on data provided, a new species Cohnella kolymensis sp.
30016228	4	33	theme	polar	624:628	arg1	profile					636:642	polar lipid profile	624:642	polar lipid profile	624:642	The chemotaxonomic characteristics (quinone system, cellular fatty acids and polar lipid profile) of strain 20.16T were consistent with members of the genus Cohnella.
30016228	0	34	theme	Cohnella	0:7	arg1	sp					20:21	Cohnella kolymensis sp	0:21	Cohnella kolymensis sp.	0:22	Cohnella kolymensis sp.
30016228	8	35	theme	predicted	1134:1142	arg1	genome					1079:1084	The genome	1075:1084	The genome	1075:1084	The genome has a G+C content of 50.5 mol% and encodes 4843 predicted genes, of these 4740 are protein-coding ones.
30016228	8	35	theme	predicted	1134:1142	arg1	genes					1144:1148	4843 predicted genes	1129:1148	4843 predicted genes	1129:1148	The genome has a G+C content of 50.5 mol% and encodes 4843 predicted genes, of these 4740 are protein-coding ones.
30016228	9	36	from	species	1341:1347	arg1	differentiation					1282:1296	clear differentiation	1276:1296	clear differentiation of strain 20.16T from the closest Cohnella species	1276:1347	The results of chemotaxonomic, physiological and biochemical characterization allowed clear differentiation of strain 20.16T from the closest Cohnella species.
30016228	6	37	theme	molar	829:833	arg1	ratio					835:839	The molar ratio	825:839	The molar ratio	825:839	The molar ratio and composition of major amino acids (meso-diaminopimelic acid, alanine, and glutamic acid) correspond to the peptydoglycan type A1γ.
30016228	3	38	theme	sequence	344:351	arg1	analysis					353:360	16S rRNA gene sequence analysis	330:360	16S rRNA gene sequence analysis	330:360	The permafrost isolate clustered with members of the genus Cohnella on the basis of 16S rRNA gene sequence analysis and showed the highest sequence similarity to Cohnella saccharovorans CJ22T (96.3 %), followed by Cohnella cellulosilytica FCN3-3T (96.0 %) and Cohnella panacarvi KCTC 13060T (96.0 %).
30016228	9	39	theme	clear	1276:1280	arg1	differentiation					1282:1296	clear differentiation	1276:1296	clear differentiation of strain 20.16T from the closest Cohnella species	1276:1347	The results of chemotaxonomic, physiological and biochemical characterization allowed clear differentiation of strain 20.16T from the closest Cohnella species.
30016228	11	40	theme	type	1476:1479	arg1	strain					1481:1486	the type strain	1472:1486	the type strain	1472:1486	nov. is proposed, with 20.16T (=VKM B-2846T=DSM 104983T) as the type strain.
30016228	3	41	theme	panacarvi	515:523	arg1	13060T					530:535	Cohnella panacarvi KCTC 13060T	506:535	Cohnella panacarvi KCTC 13060T (96.0 %)	506:544	The permafrost isolate clustered with members of the genus Cohnella on the basis of 16S rRNA gene sequence analysis and showed the highest sequence similarity to Cohnella saccharovorans CJ22T (96.3 %), followed by Cohnella cellulosilytica FCN3-3T (96.0 %) and Cohnella panacarvi KCTC 13060T (96.0 %).
30016228	3	41	theme	panacarvi	515:523	arg1	%					543:543	96.0 %	538:543	96.0 %	538:543	The permafrost isolate clustered with members of the genus Cohnella on the basis of 16S rRNA gene sequence analysis and showed the highest sequence similarity to Cohnella saccharovorans CJ22T (96.3 %), followed by Cohnella cellulosilytica FCN3-3T (96.0 %) and Cohnella panacarvi KCTC 13060T (96.0 %).
30016228	10	42	theme	Cohnella	1388:1395	arg1	sp					1408:1409	Cohnella kolymensis sp	1388:1409	Cohnella kolymensis sp	1388:1409	Based on data provided, a new species Cohnella kolymensis sp.
30016228	5	43	theme	small	785:789	arg1	amount					791:796	a small amount	783:796	a small amount of ll-diaminopimelic acid	783:822	The peptidoglycan diaminoacids included meso-diaminopimelic acid and a small amount of ll-diaminopimelic acid.
30016228	5	43	theme	small	785:789	arg1	acid					819:822	ll-diaminopimelic acid	801:822	ll-diaminopimelic acid	801:822	The peptidoglycan diaminoacids included meso-diaminopimelic acid and a small amount of ll-diaminopimelic acid.
30016228	6	44	theme	glutamic	918:925	arg1	acids					872:876	major amino acids	860:876	major amino acids (meso-diaminopimelic acid, alanine, and glutamic acid)	860:931	The molar ratio and composition of major amino acids (meso-diaminopimelic acid, alanine, and glutamic acid) correspond to the peptydoglycan type A1γ.
30016228	6	44	theme	glutamic	918:925	arg1	acid					927:930	glutamic acid	918:930	glutamic acid	918:930	The molar ratio and composition of major amino acids (meso-diaminopimelic acid, alanine, and glutamic acid) correspond to the peptydoglycan type A1γ.
30016228	8	45	dep	genes	1144:1148	arg1	4740					1160:1163	4740	1160:1163	4740	1160:1163	The genome has a G+C content of 50.5 mol% and encodes 4843 predicted genes, of these 4740 are protein-coding ones.
30016228	8	45	dep	genes	1144:1148	arg1	ones					1184:1187	protein-coding ones	1169:1187	protein-coding ones	1169:1187	The genome has a G+C content of 50.5 mol% and encodes 4843 predicted genes, of these 4740 are protein-coding ones.
30016228	2	46	theme	permafrost	180:189	arg1	cores					200:204	permafrost sediment cores	180:204	permafrost sediment cores in the Kolyma lowland, Siberia, Russia	180:243	A facultative anaerobic, rod-shaped, endospore-forming and non-motile bacterium was isolated from permafrost sediment cores in the Kolyma lowland, Siberia, Russia.
30016228	3	47	theme	KCTC	525:528	arg1	13060T					530:535	Cohnella panacarvi KCTC 13060T	506:535	Cohnella panacarvi KCTC 13060T (96.0 %)	506:544	The permafrost isolate clustered with members of the genus Cohnella on the basis of 16S rRNA gene sequence analysis and showed the highest sequence similarity to Cohnella saccharovorans CJ22T (96.3 %), followed by Cohnella cellulosilytica FCN3-3T (96.0 %) and Cohnella panacarvi KCTC 13060T (96.0 %).
30016228	3	47	theme	KCTC	525:528	arg1	%					543:543	96.0 %	538:543	96.0 %	538:543	The permafrost isolate clustered with members of the genus Cohnella on the basis of 16S rRNA gene sequence analysis and showed the highest sequence similarity to Cohnella saccharovorans CJ22T (96.3 %), followed by Cohnella cellulosilytica FCN3-3T (96.0 %) and Cohnella panacarvi KCTC 13060T (96.0 %).
30016228	4	48	theme	quinone	583:589	arg1	system					591:596	quinone system	583:596	quinone system	583:596	The chemotaxonomic characteristics (quinone system, cellular fatty acids and polar lipid profile) of strain 20.16T were consistent with members of the genus Cohnella.
30016228	3	49	theme	sequence	385:392	arg1	similarity					394:403	the highest sequence similarity	373:403	the highest sequence similarity	373:403	The permafrost isolate clustered with members of the genus Cohnella on the basis of 16S rRNA gene sequence analysis and showed the highest sequence similarity to Cohnella saccharovorans CJ22T (96.3 %), followed by Cohnella cellulosilytica FCN3-3T (96.0 %) and Cohnella panacarvi KCTC 13060T (96.0 %).
30016228	4	50	theme	strain	648:653	arg1	20.16T					655:660	strain 20.16T	648:660	strain 20.16T	648:660	The chemotaxonomic characteristics (quinone system, cellular fatty acids and polar lipid profile) of strain 20.16T were consistent with members of the genus Cohnella.
30016228	3	51	theme	permafrost	250:259	arg1	isolate					261:267	The permafrost isolate	246:267	The permafrost isolate	246:267	The permafrost isolate clustered with members of the genus Cohnella on the basis of 16S rRNA gene sequence analysis and showed the highest sequence similarity to Cohnella saccharovorans CJ22T (96.3 %), followed by Cohnella cellulosilytica FCN3-3T (96.0 %) and Cohnella panacarvi KCTC 13060T (96.0 %).
30016228	5	52	theme	ll-diaminopimelic	801:817	arg1	acid					819:822	ll-diaminopimelic acid	801:822	ll-diaminopimelic acid	801:822	The peptidoglycan diaminoacids included meso-diaminopimelic acid and a small amount of ll-diaminopimelic acid.
30016228	4	53	theme	cellular	599:606	arg1	acids					614:618	cellular fatty acids	599:618	cellular fatty acids	599:618	The chemotaxonomic characteristics (quinone system, cellular fatty acids and polar lipid profile) of strain 20.16T were consistent with members of the genus Cohnella.
30016228	2	54	theme	facultative	84:94	arg1	bacterium					152:160	A facultative anaerobic, rod-shaped, endospore-forming and non-motile bacterium	82:160	A facultative anaerobic, rod-shaped, endospore-forming and non-motile bacterium	82:160	A facultative anaerobic, rod-shaped, endospore-forming and non-motile bacterium was isolated from permafrost sediment cores in the Kolyma lowland, Siberia, Russia.
30016228	7	55	theme	20.16T	1011:1016	arg1	size					996:999	The estimated genome size	975:999	The estimated genome size of strain 20.16T	975:1016	The estimated genome size of strain 20.16T is 4.34 Mb (lower than those in other Cohnella species).
30016228	7	55	theme	20.16T	1011:1016	arg1	4.34 Mb					1021:1027	4.34 Mb	1021:1027	4.34 Mb (lower than those in other Cohnella species)	1021:1072	The estimated genome size of strain 20.16T is 4.34 Mb (lower than those in other Cohnella species).
30016228	4	56	theme	genus	698:702	arg1	Cohnella					704:711	the genus Cohnella	694:711	the genus Cohnella	694:711	The chemotaxonomic characteristics (quinone system, cellular fatty acids and polar lipid profile) of strain 20.16T were consistent with members of the genus Cohnella.
30016228	11	57	theme	=VKM	1443:1446	arg1	20.16T					1435:1440	20.16T	1435:1440	20.16T (=VKM B-2846T=DSM 104983T) as the type strain	1435:1486	nov. is proposed, with 20.16T (=VKM B-2846T=DSM 104983T) as the type strain.
30016228	11	57	theme	=VKM	1443:1446	arg1	104983T					1460:1466	=VKM B-2846T=DSM 104983T	1443:1466	=VKM B-2846T=DSM 104983T	1443:1466	nov. is proposed, with 20.16T (=VKM B-2846T=DSM 104983T) as the type strain.
30016228	5	58	theme	acid	819:822	arg1	amount					791:796	a small amount	783:796	a small amount of ll-diaminopimelic acid	783:822	The peptidoglycan diaminoacids included meso-diaminopimelic acid and a small amount of ll-diaminopimelic acid.
30016228	5	58	theme	acid	819:822	arg1	acid					819:822	ll-diaminopimelic acid	801:822	ll-diaminopimelic acid	801:822	The peptidoglycan diaminoacids included meso-diaminopimelic acid and a small amount of ll-diaminopimelic acid.
30016228	5	58	theme	acid	819:822	arg1	acid					774:777	meso-diaminopimelic acid	754:777	meso-diaminopimelic acid	754:777	The peptidoglycan diaminoacids included meso-diaminopimelic acid and a small amount of ll-diaminopimelic acid.
30016228	2	59	attach	isolated	166:173	arg2	bacterium					152:160	A facultative anaerobic, rod-shaped, endospore-forming and non-motile bacterium	82:160	A facultative anaerobic, rod-shaped, endospore-forming and non-motile bacterium	82:160	A facultative anaerobic, rod-shaped, endospore-forming and non-motile bacterium was isolated from permafrost sediment cores in the Kolyma lowland, Siberia, Russia.
30016228	2	59	attach	isolated	166:173	arg1	cores					200:204	permafrost sediment cores	180:204	permafrost sediment cores in the Kolyma lowland, Siberia, Russia	180:243	A facultative anaerobic, rod-shaped, endospore-forming and non-motile bacterium was isolated from permafrost sediment cores in the Kolyma lowland, Siberia, Russia.
30016228	3	60	theme	rRNA	334:337	arg1	analysis					353:360	16S rRNA gene sequence analysis	330:360	16S rRNA gene sequence analysis	330:360	The permafrost isolate clustered with members of the genus Cohnella on the basis of 16S rRNA gene sequence analysis and showed the highest sequence similarity to Cohnella saccharovorans CJ22T (96.3 %), followed by Cohnella cellulosilytica FCN3-3T (96.0 %) and Cohnella panacarvi KCTC 13060T (96.0 %).
30016228	5	61	theme	meso-diaminopimelic	754:772	arg1	acid					774:777	meso-diaminopimelic acid	754:777	meso-diaminopimelic acid	754:777	The peptidoglycan diaminoacids included meso-diaminopimelic acid and a small amount of ll-diaminopimelic acid.
30016228	4	62	dep	system	591:596	arg1	acids					614:618	cellular fatty acids	599:618	cellular fatty acids	599:618	The chemotaxonomic characteristics (quinone system, cellular fatty acids and polar lipid profile) of strain 20.16T were consistent with members of the genus Cohnella.
30016228	4	62	dep	system	591:596	arg1	profile					636:642	polar lipid profile	624:642	polar lipid profile	624:642	The chemotaxonomic characteristics (quinone system, cellular fatty acids and polar lipid profile) of strain 20.16T were consistent with members of the genus Cohnella.
30016228	4	63	theme	20.16T	655:660	arg1	characteristics					566:580	The chemotaxonomic characteristics	547:580	The chemotaxonomic characteristics (quinone system, cellular fatty acids and polar lipid profile) of strain 20.16T	547:660	The chemotaxonomic characteristics (quinone system, cellular fatty acids and polar lipid profile) of strain 20.16T were consistent with members of the genus Cohnella.
30016228	4	63	theme	20.16T	655:660	arg1	consistent					667:676	consistent	667:676	consistent	667:676	The chemotaxonomic characteristics (quinone system, cellular fatty acids and polar lipid profile) of strain 20.16T were consistent with members of the genus Cohnella.
30016228	3	64	theme	highest	377:383	arg1	similarity					394:403	the highest sequence similarity	373:403	the highest sequence similarity	373:403	The permafrost isolate clustered with members of the genus Cohnella on the basis of 16S rRNA gene sequence analysis and showed the highest sequence similarity to Cohnella saccharovorans CJ22T (96.3 %), followed by Cohnella cellulosilytica FCN3-3T (96.0 %) and Cohnella panacarvi KCTC 13060T (96.0 %).
30016228	3	65	theme	genus	299:303	arg1	Cohnella					305:312	the genus Cohnella	295:312	the genus Cohnella	295:312	The permafrost isolate clustered with members of the genus Cohnella on the basis of 16S rRNA gene sequence analysis and showed the highest sequence similarity to Cohnella saccharovorans CJ22T (96.3 %), followed by Cohnella cellulosilytica FCN3-3T (96.0 %) and Cohnella panacarvi KCTC 13060T (96.0 %).
30016228	3	66	theme	Cohnella	460:467	arg1	FCN3-3T					485:491	Cohnella cellulosilytica FCN3-3T	460:491	Cohnella cellulosilytica FCN3-3T (96.0 %)	460:500	The permafrost isolate clustered with members of the genus Cohnella on the basis of 16S rRNA gene sequence analysis and showed the highest sequence similarity to Cohnella saccharovorans CJ22T (96.3 %), followed by Cohnella cellulosilytica FCN3-3T (96.0 %) and Cohnella panacarvi KCTC 13060T (96.0 %).
30016228	3	66	theme	Cohnella	460:467	arg1	%					499:499	96.0 %	494:499	96.0 %	494:499	The permafrost isolate clustered with members of the genus Cohnella on the basis of 16S rRNA gene sequence analysis and showed the highest sequence similarity to Cohnella saccharovorans CJ22T (96.3 %), followed by Cohnella cellulosilytica FCN3-3T (96.0 %) and Cohnella panacarvi KCTC 13060T (96.0 %).
30016228	10	67	dep	sp	1408:1409	arg1	species					1380:1386	a new species	1374:1386	a new species	1374:1386	Based on data provided, a new species Cohnella kolymensis sp.
30016228	7	68	theme	estimated	979:987	arg1	size					996:999	The estimated genome size	975:999	The estimated genome size of strain 20.16T	975:1016	The estimated genome size of strain 20.16T is 4.34 Mb (lower than those in other Cohnella species).
30016228	7	68	theme	estimated	979:987	arg1	4.34 Mb					1021:1027	4.34 Mb	1021:1027	4.34 Mb (lower than those in other Cohnella species)	1021:1072	The estimated genome size of strain 20.16T is 4.34 Mb (lower than those in other Cohnella species).
30016228	4	69	dep	characteristics	566:580	arg1	system					591:596	quinone system	583:596	quinone system	583:596	The chemotaxonomic characteristics (quinone system, cellular fatty acids and polar lipid profile) of strain 20.16T were consistent with members of the genus Cohnella.
30016228	9	70	theme	closest	1324:1330	arg1	species					1341:1347	the closest Cohnella species	1320:1347	the closest Cohnella species	1320:1347	The results of chemotaxonomic, physiological and biochemical characterization allowed clear differentiation of strain 20.16T from the closest Cohnella species.
30016228	4	71	theme	chemotaxonomic	551:564	arg1	characteristics					566:580	The chemotaxonomic characteristics	547:580	The chemotaxonomic characteristics (quinone system, cellular fatty acids and polar lipid profile) of strain 20.16T	547:660	The chemotaxonomic characteristics (quinone system, cellular fatty acids and polar lipid profile) of strain 20.16T were consistent with members of the genus Cohnella.
30016228	4	71	theme	chemotaxonomic	551:564	arg1	consistent					667:676	consistent	667:676	consistent	667:676	The chemotaxonomic characteristics (quinone system, cellular fatty acids and polar lipid profile) of strain 20.16T were consistent with members of the genus Cohnella.
30016228	3	72	dep	Cohnella	460:467	arg1	cellulosilytica					469:483	cellulosilytica	469:483	cellulosilytica	469:483	The permafrost isolate clustered with members of the genus Cohnella on the basis of 16S rRNA gene sequence analysis and showed the highest sequence similarity to Cohnella saccharovorans CJ22T (96.3 %), followed by Cohnella cellulosilytica FCN3-3T (96.0 %) and Cohnella panacarvi KCTC 13060T (96.0 %).
30016228	8	73	theme	50.5 mol	1107:1114	arg1	%					1115:1115	50.5 mol%	1107:1115	50.5 mol%	1107:1115	The genome has a G+C content of 50.5 mol% and encodes 4843 predicted genes, of these 4740 are protein-coding ones.
30016228	9	74	theme	characterization	1251:1266	arg1	results					1194:1200	The results	1190:1200	The results of chemotaxonomic, physiological and biochemical characterization	1190:1266	The results of chemotaxonomic, physiological and biochemical characterization allowed clear differentiation of strain 20.16T from the closest Cohnella species.
30016228	9	75	theme	strain	1301:1306	arg1	20.16T					1308:1313	strain 20.16T	1301:1313	strain 20.16T	1301:1313	The results of chemotaxonomic, physiological and biochemical characterization allowed clear differentiation of strain 20.16T from the closest Cohnella species.
30016228	9	76	theme	Cohnella	1332:1339	arg1	species					1341:1347	the closest Cohnella species	1320:1347	the closest Cohnella species	1320:1347	The results of chemotaxonomic, physiological and biochemical characterization allowed clear differentiation of strain 20.16T from the closest Cohnella species.
30016228	6	77	theme	meso-diaminopimelic	879:897	arg1	acids					872:876	major amino acids	860:876	major amino acids (meso-diaminopimelic acid, alanine, and glutamic acid)	860:931	The molar ratio and composition of major amino acids (meso-diaminopimelic acid, alanine, and glutamic acid) correspond to the peptydoglycan type A1γ.
30016228	6	77	theme	meso-diaminopimelic	879:897	arg1	acid					899:902	meso-diaminopimelic acid	879:902	meso-diaminopimelic acid	879:902	The molar ratio and composition of major amino acids (meso-diaminopimelic acid, alanine, and glutamic acid) correspond to the peptydoglycan type A1γ.
30016228	2	78	theme	sediment	191:198	arg1	cores					200:204	permafrost sediment cores	180:204	permafrost sediment cores in the Kolyma lowland, Siberia, Russia	180:243	A facultative anaerobic, rod-shaped, endospore-forming and non-motile bacterium was isolated from permafrost sediment cores in the Kolyma lowland, Siberia, Russia.
30016228	9	79	theme	chemotaxonomic	1205:1218	arg1	characterization					1251:1266	chemotaxonomic, physiological and biochemical characterization	1205:1266	chemotaxonomic, physiological and biochemical characterization	1205:1266	The results of chemotaxonomic, physiological and biochemical characterization allowed clear differentiation of strain 20.16T from the closest Cohnella species.
30016228	6	80	theme	amino	866:870	arg1	alanine					905:911	alanine	905:911	alanine	905:911	The molar ratio and composition of major amino acids (meso-diaminopimelic acid, alanine, and glutamic acid) correspond to the peptydoglycan type A1γ.
30016228	6	80	theme	amino	866:870	arg1	acids					872:876	major amino acids	860:876	major amino acids (meso-diaminopimelic acid, alanine, and glutamic acid)	860:931	The molar ratio and composition of major amino acids (meso-diaminopimelic acid, alanine, and glutamic acid) correspond to the peptydoglycan type A1γ.
30016228	6	80	theme	amino	866:870	arg1	acid					899:902	meso-diaminopimelic acid	879:902	meso-diaminopimelic acid	879:902	The molar ratio and composition of major amino acids (meso-diaminopimelic acid, alanine, and glutamic acid) correspond to the peptydoglycan type A1γ.
30016228	6	80	theme	amino	866:870	arg1	acid					927:930	glutamic acid	918:930	glutamic acid	918:930	The molar ratio and composition of major amino acids (meso-diaminopimelic acid, alanine, and glutamic acid) correspond to the peptydoglycan type A1γ.
30016228	4	81	with	consistent	667:676	arg1	members					683:689	members	683:689	members of the genus Cohnella	683:711	The chemotaxonomic characteristics (quinone system, cellular fatty acids and polar lipid profile) of strain 20.16T were consistent with members of the genus Cohnella.
30016228	7	82	theme	strain	1004:1009	arg1	20.16T					1011:1016	strain 20.16T	1004:1016	strain 20.16T	1004:1016	The estimated genome size of strain 20.16T is 4.34 Mb (lower than those in other Cohnella species).
30016228	4	83	theme	lipid	630:634	arg1	profile					636:642	polar lipid profile	624:642	polar lipid profile	624:642	The chemotaxonomic characteristics (quinone system, cellular fatty acids and polar lipid profile) of strain 20.16T were consistent with members of the genus Cohnella.
30016228	6	84	theme	type	965:968	arg1	A1γ					970:972	the peptydoglycan type A1γ	947:972	the peptydoglycan type A1γ	947:972	The molar ratio and composition of major amino acids (meso-diaminopimelic acid, alanine, and glutamic acid) correspond to the peptydoglycan type A1γ.
31070848	0	0	theme	birth	77:81	arg1	prevention					83:92	preterm birth prevention	69:92	preterm birth prevention	69:92	Ex vivo pregnant-like tissue model to assess injectable hydrogel for preterm birth prevention.
31070848	5	1	from	decrease	770:777	arg1	organization					791:802	collagen organization	782:802	collagen organization	782:802	Enzymatic treatment led to an 86 ± 7.9% decrease in modulus, which correlated to a decrease in collagen organization as observed by differences in collagen birefringence.
31070848	7	2	theme	tissue	1122:1127	arg1	properties					1140:1149	tissue mechanical properties	1122:1149	tissue mechanical properties	1122:1149	After injection, the mechanical properties and volume increase of the hydrogel-treated tissue were measured resulting in a 54 ± 16% volume increase with minimal effect on tissue mechanical properties.
31070848	2	3	theme	alternative	314:324	arg1	treatments					326:335	alternative treatments	314:335	alternative treatments	314:335	Standard treatment for CI is cerclage, which has variable success rates, resulting in a clinical need for alternative treatments.
31070848	0	4	theme	preterm	69:75	arg1	prevention					83:92	preterm birth prevention	69:92	preterm birth prevention	69:92	Ex vivo pregnant-like tissue model to assess injectable hydrogel for preterm birth prevention.
31070848	7	5	theme	volume	1083:1088	arg1	increase					1090:1097	a 54 ± 16% volume increase	1072:1097	a 54 ± 16% volume increase with minimal effect on tissue mechanical properties	1072:1149	After injection, the mechanical properties and volume increase of the hydrogel-treated tissue were measured resulting in a 54 ± 16% volume increase with minimal effect on tissue mechanical properties.
31070848	3	6	theme	novel	460:464	arg1	hydrogel					446:453	an injectable silk-based hydrogel	421:453	an injectable silk-based hydrogel	421:453	Our objective was to develop an ex vivo model of softened cervical tissue to study an injectable silk-based hydrogel as a novel alternative treatment for CI.
31070848	3	6	theme	novel	460:464	arg1	treatment					478:486	a novel alternative treatment	458:486	a novel alternative treatment for CI	458:493	Our objective was to develop an ex vivo model of softened cervical tissue to study an injectable silk-based hydrogel as a novel alternative treatment for CI.
31070848	9	7	theme	silk-based	1444:1453	arg1	hydrogels					1455:1463	silk-based hydrogels	1444:1463	silk-based hydrogels	1444:1463	Overall, this study developed an ex vivo pregnant-like human tissue model to assess cervical augmentation and showed the potential of silk-based hydrogels as an alternative treatment for cervical insufficiency.
31070848	5	8	from	decrease	727:734	arg1	modulus					739:745	modulus	739:745	modulus	739:745	Enzymatic treatment led to an 86 ± 7.9% decrease in modulus, which correlated to a decrease in collagen organization as observed by differences in collagen birefringence.
31070848	9	9	dep	ex	1343:1344	arg1	vivo					1346:1349	vivo	1346:1349	vivo	1346:1349	Overall, this study developed an ex vivo pregnant-like human tissue model to assess cervical augmentation and showed the potential of silk-based hydrogels as an alternative treatment for cervical insufficiency.
31070848	2	10	theme	variable	257:264	arg1	rates					274:278	variable success rates	257:278	variable success rates	257:278	Standard treatment for CI is cerclage, which has variable success rates, resulting in a clinical need for alternative treatments.
31070848	9	11	theme	hydrogels	1455:1463	arg1	potential					1431:1439	the potential	1427:1439	the potential of silk-based hydrogels as an alternative treatment for cervical insufficiency	1427:1518	Overall, this study developed an ex vivo pregnant-like human tissue model to assess cervical augmentation and showed the potential of silk-based hydrogels as an alternative treatment for cervical insufficiency.
31070848	5	12	theme	86 ± 7.9	717:724	arg1	%					725:725	%	725:725	%	725:725	Enzymatic treatment led to an 86 ± 7.9% decrease in modulus, which correlated to a decrease in collagen organization as observed by differences in collagen birefringence.
31070848	6	13	theme	composite	931:939	arg1	hydrogel					941:948	a crosslinked silk-hyaluronic acid composite hydrogel	896:948	a crosslinked silk-hyaluronic acid composite hydrogel	896:948	The softened tissue was injected with a crosslinked silk-hyaluronic acid composite hydrogel.
31070848	5	14	theme	collagen	834:841	arg1	birefringence					843:855	collagen birefringence	834:855	collagen birefringence	834:855	Enzymatic treatment led to an 86 ± 7.9% decrease in modulus, which correlated to a decrease in collagen organization as observed by differences in collagen birefringence.
31070848	5	15	theme	%	725:725	arg1	decrease					727:734	an 86 ± 7.9% decrease	714:734	an 86 ± 7.9% decrease	714:734	Enzymatic treatment led to an 86 ± 7.9% decrease in modulus, which correlated to a decrease in collagen organization as observed by differences in collagen birefringence.
31070848	1	16	theme	preterm	148:154	arg1	birth					156:160	preterm birth	148:160	preterm birth	148:160	Cervical insufficiency (CI) is an important cause of preterm birth, which leads to severe newborn complications.
31070848	9	17	theme	ex	1343:1344	arg1	model					1378:1382	an ex vivo pregnant-like human tissue model	1340:1382	an ex vivo pregnant-like human tissue model to assess cervical augmentation	1340:1414	Overall, this study developed an ex vivo pregnant-like human tissue model to assess cervical augmentation and showed the potential of silk-based hydrogels as an alternative treatment for cervical insufficiency.
31070848	7	18	theme	minimal	1104:1110	arg1	effect					1112:1117	minimal effect	1104:1117	minimal effect on tissue mechanical properties	1104:1149	After injection, the mechanical properties and volume increase of the hydrogel-treated tissue were measured resulting in a 54 ± 16% volume increase with minimal effect on tissue mechanical properties.
31070848	5	19	theme	collagen	782:789	arg1	organization					791:802	collagen organization	782:802	collagen organization	782:802	Enzymatic treatment led to an 86 ± 7.9% decrease in modulus, which correlated to a decrease in collagen organization as observed by differences in collagen birefringence.
31070848	1	20	theme	birth	156:160	arg1	cause					139:143	an important cause	126:143	an important cause	126:143	Cervical insufficiency (CI) is an important cause of preterm birth, which leads to severe newborn complications.
31070848	1	20	theme	birth	156:160	arg1	insufficiency					104:116	Cervical insufficiency	95:116	Cervical insufficiency (CI)	95:121	Cervical insufficiency (CI) is an important cause of preterm birth, which leads to severe newborn complications.
31070848	4	21	from	women	529:533	arg1	tissue					505:510	Cervical tissue	496:510	Cervical tissue from nonpregnant women	496:533	Cervical tissue from nonpregnant women was enzymatically treated and characterized to determine tissue hydration, collagen organization, and mechanical properties via unconfined compression.
31070848	6	22	theme	softened	862:869	arg1	tissue					871:876	The softened tissue	858:876	The softened tissue	858:876	The softened tissue was injected with a crosslinked silk-hyaluronic acid composite hydrogel.
31070848	3	23	dep	ex	370:371	arg1	vivo					373:376	vivo	373:376	vivo	373:376	Our objective was to develop an ex vivo model of softened cervical tissue to study an injectable silk-based hydrogel as a novel alternative treatment for CI.
31070848	8	24	theme	metabolic	1278:1286	arg1	activity					1288:1295	metabolic activity	1278:1295	metabolic activity	1278:1295	In addition, cervical fibroblasts on silk-hyaluronic acid hydrogels remained viable and exhibited increased proliferation and metabolic activity over 5 days.
31070848	0	25	theme	Ex	0:1	arg1	model					29:33	Ex vivo pregnant-like tissue model	0:33	Ex vivo pregnant-like tissue model	0:33	Ex vivo pregnant-like tissue model to assess injectable hydrogel for preterm birth prevention.
31070848	9	26	theme	pregnant-like	1351:1363	arg1	model					1378:1382	an ex vivo pregnant-like human tissue model	1340:1382	an ex vivo pregnant-like human tissue model to assess cervical augmentation	1340:1414	Overall, this study developed an ex vivo pregnant-like human tissue model to assess cervical augmentation and showed the potential of silk-based hydrogels as an alternative treatment for cervical insufficiency.
31070848	9	27	theme	cervical	1394:1401	arg1	augmentation					1403:1414	cervical augmentation	1394:1414	cervical augmentation	1394:1414	Overall, this study developed an ex vivo pregnant-like human tissue model to assess cervical augmentation and showed the potential of silk-based hydrogels as an alternative treatment for cervical insufficiency.
31070848	4	28	theme	mechanical	637:646	arg1	properties					648:657	mechanical properties	637:657	mechanical properties	637:657	Cervical tissue from nonpregnant women was enzymatically treated and characterized to determine tissue hydration, collagen organization, and mechanical properties via unconfined compression.
31070848	0	29	theme	tissue	22:27	arg1	model					29:33	Ex vivo pregnant-like tissue model	0:33	Ex vivo pregnant-like tissue model	0:33	Ex vivo pregnant-like tissue model to assess injectable hydrogel for preterm birth prevention.
31070848	9	30	theme	human	1365:1369	arg1	model					1378:1382	an ex vivo pregnant-like human tissue model	1340:1382	an ex vivo pregnant-like human tissue model to assess cervical augmentation	1340:1414	Overall, this study developed an ex vivo pregnant-like human tissue model to assess cervical augmentation and showed the potential of silk-based hydrogels as an alternative treatment for cervical insufficiency.
31070848	5	31	theme	Enzymatic	687:695	arg1	treatment					697:705	Enzymatic treatment	687:705	Enzymatic treatment	687:705	Enzymatic treatment led to an 86 ± 7.9% decrease in modulus, which correlated to a decrease in collagen organization as observed by differences in collagen birefringence.
31070848	1	32	theme	Cervical	95:102	arg1	cause					139:143	an important cause	126:143	an important cause	126:143	Cervical insufficiency (CI) is an important cause of preterm birth, which leads to severe newborn complications.
31070848	1	32	theme	Cervical	95:102	arg1	CI					119:120	CI	119:120	CI	119:120	Cervical insufficiency (CI) is an important cause of preterm birth, which leads to severe newborn complications.
31070848	1	32	theme	Cervical	95:102	arg1	insufficiency					104:116	Cervical insufficiency	95:116	Cervical insufficiency (CI)	95:121	Cervical insufficiency (CI) is an important cause of preterm birth, which leads to severe newborn complications.
31070848	7	33	with	increase	1090:1097	arg1	effect					1112:1117	minimal effect	1104:1117	minimal effect on tissue mechanical properties	1104:1149	After injection, the mechanical properties and volume increase of the hydrogel-treated tissue were measured resulting in a 54 ± 16% volume increase with minimal effect on tissue mechanical properties.
31070848	9	34	theme	cervical	1497:1504	arg1	insufficiency					1506:1518	cervical insufficiency	1497:1518	cervical insufficiency	1497:1518	Overall, this study developed an ex vivo pregnant-like human tissue model to assess cervical augmentation and showed the potential of silk-based hydrogels as an alternative treatment for cervical insufficiency.
31070848	0	35	theme	pregnant-like	8:20	arg1	model					29:33	Ex vivo pregnant-like tissue model	0:33	Ex vivo pregnant-like tissue model	0:33	Ex vivo pregnant-like tissue model to assess injectable hydrogel for preterm birth prevention.
31070848	2	36	contain	has	253:255	arg2	rates					274:278	variable success rates	257:278	variable success rates	257:278	Standard treatment for CI is cerclage, which has variable success rates, resulting in a clinical need for alternative treatments.
31070848	2	36	contain	has	253:255	arg1	cerclage					237:244	cerclage	237:244	cerclage	237:244	Standard treatment for CI is cerclage, which has variable success rates, resulting in a clinical need for alternative treatments.
31070848	7	37	theme	%	1081:1081	arg1	increase					1090:1097	a 54 ± 16% volume increase	1072:1097	a 54 ± 16% volume increase with minimal effect on tissue mechanical properties	1072:1149	After injection, the mechanical properties and volume increase of the hydrogel-treated tissue were measured resulting in a 54 ± 16% volume increase with minimal effect on tissue mechanical properties.
31070848	2	38	theme	success	266:272	arg1	rates					274:278	variable success rates	257:278	variable success rates	257:278	Standard treatment for CI is cerclage, which has variable success rates, resulting in a clinical need for alternative treatments.
31070848	6	39	theme	crosslinked	898:908	arg1	hydrogel					941:948	a crosslinked silk-hyaluronic acid composite hydrogel	896:948	a crosslinked silk-hyaluronic acid composite hydrogel	896:948	The softened tissue was injected with a crosslinked silk-hyaluronic acid composite hydrogel.
31070848	4	40	theme	unconfined	663:672	arg1	compression					674:684	unconfined compression	663:684	unconfined compression	663:684	Cervical tissue from nonpregnant women was enzymatically treated and characterized to determine tissue hydration, collagen organization, and mechanical properties via unconfined compression.
31070848	3	41	theme	ex	370:371	arg1	model					378:382	an ex vivo model	367:382	an ex vivo model of softened cervical tissue to study an injectable silk-based hydrogel as a novel alternative treatment for CI	367:493	Our objective was to develop an ex vivo model of softened cervical tissue to study an injectable silk-based hydrogel as a novel alternative treatment for CI.
31070848	4	42	theme	nonpregnant	517:527	arg1	women					529:533	nonpregnant women	517:533	nonpregnant women	517:533	Cervical tissue from nonpregnant women was enzymatically treated and characterized to determine tissue hydration, collagen organization, and mechanical properties via unconfined compression.
31070848	3	43	theme	tissue	405:410	arg1	model					378:382	an ex vivo model	367:382	an ex vivo model of softened cervical tissue to study an injectable silk-based hydrogel as a novel alternative treatment for CI	367:493	Our objective was to develop an ex vivo model of softened cervical tissue to study an injectable silk-based hydrogel as a novel alternative treatment for CI.
31070848	8	44	theme	acid	1205:1208	arg1	hydrogels					1210:1218	silk-hyaluronic acid hydrogels	1189:1218	silk-hyaluronic acid hydrogels	1189:1218	In addition, cervical fibroblasts on silk-hyaluronic acid hydrogels remained viable and exhibited increased proliferation and metabolic activity over 5 days.
31070848	6	45	link	crosslinked	898:908	arg1	hydrogel					941:948	a crosslinked silk-hyaluronic acid composite hydrogel	896:948	a crosslinked silk-hyaluronic acid composite hydrogel	896:948	The softened tissue was injected with a crosslinked silk-hyaluronic acid composite hydrogel.
31070848	1	46	theme	severe	178:183	arg1	complications					193:205	severe newborn complications	178:205	severe newborn complications	178:205	Cervical insufficiency (CI) is an important cause of preterm birth, which leads to severe newborn complications.
31070848	3	47	theme	softened	387:394	arg1	tissue					405:410	softened cervical tissue	387:410	softened cervical tissue	387:410	Our objective was to develop an ex vivo model of softened cervical tissue to study an injectable silk-based hydrogel as a novel alternative treatment for CI.
31070848	7	48	from	effect	1112:1117	arg1	properties					1140:1149	tissue mechanical properties	1122:1149	tissue mechanical properties	1122:1149	After injection, the mechanical properties and volume increase of the hydrogel-treated tissue were measured resulting in a 54 ± 16% volume increase with minimal effect on tissue mechanical properties.
31070848	2	49	theme	Standard	208:215	arg1	treatment					217:225	Standard treatment	208:225	Standard treatment for CI	208:232	Standard treatment for CI is cerclage, which has variable success rates, resulting in a clinical need for alternative treatments.
31070848	5	50	from	differences	819:829	arg1	birefringence					843:855	collagen birefringence	834:855	collagen birefringence	834:855	Enzymatic treatment led to an 86 ± 7.9% decrease in modulus, which correlated to a decrease in collagen organization as observed by differences in collagen birefringence.
31070848	1	51	theme	newborn	185:191	arg1	complications					193:205	severe newborn complications	178:205	severe newborn complications	178:205	Cervical insufficiency (CI) is an important cause of preterm birth, which leads to severe newborn complications.
31070848	0	52	theme	injectable	45:54	arg1	hydrogel					56:63	injectable hydrogel	45:63	injectable hydrogel for preterm birth prevention	45:92	Ex vivo pregnant-like tissue model to assess injectable hydrogel for preterm birth prevention.
31070848	8	53	theme	cervical	1165:1172	arg1	fibroblasts					1174:1184	cervical fibroblasts	1165:1184	cervical fibroblasts on silk-hyaluronic acid hydrogels	1165:1218	In addition, cervical fibroblasts on silk-hyaluronic acid hydrogels remained viable and exhibited increased proliferation and metabolic activity over 5 days.
31070848	7	54	theme	hydrogel-treated	1021:1036	arg1	tissue					1038:1043	the hydrogel-treated tissue	1017:1043	the hydrogel-treated tissue	1017:1043	After injection, the mechanical properties and volume increase of the hydrogel-treated tissue were measured resulting in a 54 ± 16% volume increase with minimal effect on tissue mechanical properties.
31070848	4	55	theme	Cervical	496:503	arg1	tissue					505:510	Cervical tissue	496:510	Cervical tissue from nonpregnant women	496:533	Cervical tissue from nonpregnant women was enzymatically treated and characterized to determine tissue hydration, collagen organization, and mechanical properties via unconfined compression.
31070848	7	56	theme	mechanical	1129:1138	arg1	properties					1140:1149	tissue mechanical properties	1122:1149	tissue mechanical properties	1122:1149	After injection, the mechanical properties and volume increase of the hydrogel-treated tissue were measured resulting in a 54 ± 16% volume increase with minimal effect on tissue mechanical properties.
31070848	2	57	theme	clinical	296:303	arg1	need					305:308	a clinical need	294:308	a clinical need for alternative treatments	294:335	Standard treatment for CI is cerclage, which has variable success rates, resulting in a clinical need for alternative treatments.
31070848	6	58	theme	acid	926:929	arg1	hydrogel					941:948	a crosslinked silk-hyaluronic acid composite hydrogel	896:948	a crosslinked silk-hyaluronic acid composite hydrogel	896:948	The softened tissue was injected with a crosslinked silk-hyaluronic acid composite hydrogel.
31070848	7	59	theme	tissue	1038:1043	arg1	properties					983:992	the mechanical properties	968:992	the mechanical properties	968:992	After injection, the mechanical properties and volume increase of the hydrogel-treated tissue were measured resulting in a 54 ± 16% volume increase with minimal effect on tissue mechanical properties.
31070848	7	59	theme	tissue	1038:1043	arg1	increase					1005:1012	volume increase	998:1012	volume increase	998:1012	After injection, the mechanical properties and volume increase of the hydrogel-treated tissue were measured resulting in a 54 ± 16% volume increase with minimal effect on tissue mechanical properties.
31070848	8	60	from	fibroblasts	1174:1184	arg1	hydrogels					1210:1218	silk-hyaluronic acid hydrogels	1189:1218	silk-hyaluronic acid hydrogels	1189:1218	In addition, cervical fibroblasts on silk-hyaluronic acid hydrogels remained viable and exhibited increased proliferation and metabolic activity over 5 days.
31070848	9	61	theme	tissue	1371:1376	arg1	model					1378:1382	an ex vivo pregnant-like human tissue model	1340:1382	an ex vivo pregnant-like human tissue model to assess cervical augmentation	1340:1414	Overall, this study developed an ex vivo pregnant-like human tissue model to assess cervical augmentation and showed the potential of silk-based hydrogels as an alternative treatment for cervical insufficiency.
31070848	3	62	theme	injectable	424:433	arg1	hydrogel					446:453	an injectable silk-based hydrogel	421:453	an injectable silk-based hydrogel	421:453	Our objective was to develop an ex vivo model of softened cervical tissue to study an injectable silk-based hydrogel as a novel alternative treatment for CI.
31070848	3	62	theme	injectable	424:433	arg1	treatment					478:486	a novel alternative treatment	458:486	a novel alternative treatment for CI	458:493	Our objective was to develop an ex vivo model of softened cervical tissue to study an injectable silk-based hydrogel as a novel alternative treatment for CI.
31070848	9	63	theme	alternative	1471:1481	arg1	treatment					1483:1491	an alternative treatment	1468:1491	an alternative treatment for cervical insufficiency	1468:1518	Overall, this study developed an ex vivo pregnant-like human tissue model to assess cervical augmentation and showed the potential of silk-based hydrogels as an alternative treatment for cervical insufficiency.
31070848	8	64	theme	silk-hyaluronic	1189:1203	arg1	hydrogels					1210:1218	silk-hyaluronic acid hydrogels	1189:1218	silk-hyaluronic acid hydrogels	1189:1218	In addition, cervical fibroblasts on silk-hyaluronic acid hydrogels remained viable and exhibited increased proliferation and metabolic activity over 5 days.
31070848	3	65	theme	silk-based	435:444	arg1	hydrogel					446:453	an injectable silk-based hydrogel	421:453	an injectable silk-based hydrogel	421:453	Our objective was to develop an ex vivo model of softened cervical tissue to study an injectable silk-based hydrogel as a novel alternative treatment for CI.
31070848	3	65	theme	silk-based	435:444	arg1	treatment					478:486	a novel alternative treatment	458:486	a novel alternative treatment for CI	458:493	Our objective was to develop an ex vivo model of softened cervical tissue to study an injectable silk-based hydrogel as a novel alternative treatment for CI.
31070848	4	66	theme	collagen	610:617	arg1	organization					619:630	collagen organization	610:630	collagen organization	610:630	Cervical tissue from nonpregnant women was enzymatically treated and characterized to determine tissue hydration, collagen organization, and mechanical properties via unconfined compression.
31070848	7	67	theme	volume	998:1003	arg1	increase					1005:1012	volume increase	998:1012	volume increase	998:1012	After injection, the mechanical properties and volume increase of the hydrogel-treated tissue were measured resulting in a 54 ± 16% volume increase with minimal effect on tissue mechanical properties.
31070848	3	68	theme	alternative	466:476	arg1	hydrogel					446:453	an injectable silk-based hydrogel	421:453	an injectable silk-based hydrogel	421:453	Our objective was to develop an ex vivo model of softened cervical tissue to study an injectable silk-based hydrogel as a novel alternative treatment for CI.
31070848	3	68	theme	alternative	466:476	arg1	treatment					478:486	a novel alternative treatment	458:486	a novel alternative treatment for CI	458:493	Our objective was to develop an ex vivo model of softened cervical tissue to study an injectable silk-based hydrogel as a novel alternative treatment for CI.
31070848	3	69	theme	cervical	396:403	arg1	tissue					405:410	softened cervical tissue	387:410	softened cervical tissue	387:410	Our objective was to develop an ex vivo model of softened cervical tissue to study an injectable silk-based hydrogel as a novel alternative treatment for CI.
31070848	4	70	theme	tissue	592:597	arg1	hydration					599:607	tissue hydration	592:607	tissue hydration	592:607	Cervical tissue from nonpregnant women was enzymatically treated and characterized to determine tissue hydration, collagen organization, and mechanical properties via unconfined compression.
31070848	8	71	theme	increased	1250:1258	arg1	proliferation					1260:1272	increased proliferation	1250:1272	increased proliferation	1250:1272	In addition, cervical fibroblasts on silk-hyaluronic acid hydrogels remained viable and exhibited increased proliferation and metabolic activity over 5 days.
31070848	6	72	theme	silk-hyaluronic	910:924	arg1	hydrogel					941:948	a crosslinked silk-hyaluronic acid composite hydrogel	896:948	a crosslinked silk-hyaluronic acid composite hydrogel	896:948	The softened tissue was injected with a crosslinked silk-hyaluronic acid composite hydrogel.
31070848	0	73	dep	Ex	0:1	arg1	vivo					3:6	vivo	3:6	vivo	3:6	Ex vivo pregnant-like tissue model to assess injectable hydrogel for preterm birth prevention.
31070848	7	74	theme	mechanical	972:981	arg1	properties					983:992	the mechanical properties	968:992	the mechanical properties	968:992	After injection, the mechanical properties and volume increase of the hydrogel-treated tissue were measured resulting in a 54 ± 16% volume increase with minimal effect on tissue mechanical properties.
31070848	1	75	theme	important	129:137	arg1	cause					139:143	an important cause	126:143	an important cause	126:143	Cervical insufficiency (CI) is an important cause of preterm birth, which leads to severe newborn complications.
31070848	1	75	theme	important	129:137	arg1	insufficiency					104:116	Cervical insufficiency	95:116	Cervical insufficiency (CI)	95:121	Cervical insufficiency (CI) is an important cause of preterm birth, which leads to severe newborn complications.
31628537	6	0	theme	predominant	919:929	arg1	abundance					931:939	the predominant abundance	915:939	the predominant abundance of microplastics, such as polyamides (up to 53.3%), polyethylene and polypropylene (up to 17.1%), and natural fragments such as cellulose (up to 45.8%) and wool (up 18.8%) in the same size range	915:1134	FTIR results showed the predominant abundance of microplastics, such as polyamides (up to 53.3%), polyethylene and polypropylene (up to 17.1%), and natural fragments such as cellulose (up to 45.8%) and wool (up 18.8%) in the same size range.
31628537	4	1	dep	transform	688:696	arg1	infrared					698:705	infrared	698:705	transform infrared spectroscopy (FTIR) 2D imaging	688:736	MPs were analysed and identified by a non-destructive method using Fourier transform infrared spectroscopy (FTIR) 2D imaging.
31628537	6	2	dep	45.8	1086:1089	arg1	to					1083:1084	to	1083:1084	to	1083:1084	FTIR results showed the predominant abundance of microplastics, such as polyamides (up to 53.3%), polyethylene and polypropylene (up to 17.1%), and natural fragments such as cellulose (up to 45.8%) and wool (up 18.8%) in the same size range.
31628537	0	3	from	characterisation	53:68	arg1	lake					83:86	lake	83:86	lake	83:86	Assessment of microplastic pollution: occurrence and characterisation in Vesijärvi lake and Pikku Vesijärvi pond, Finland.
31628537	0	3	from	characterisation	53:68	arg1	Vesijärvi					98:106	Vesijärvi	98:106	Vesijärvi	98:106	Assessment of microplastic pollution: occurrence and characterisation in Vesijärvi lake and Pikku Vesijärvi pond, Finland.
31628537	7	4	theme	recreational	1205:1216	arg1	activities					1218:1227	recreational activities	1205:1227	recreational activities	1205:1227	The potential release of MPs arising from stormwaters and sport and recreational activities was evidenced.
31628537	6	5	theme	size	1125:1128	arg1	range					1130:1134	the same size range	1116:1134	the same size range	1116:1134	FTIR results showed the predominant abundance of microplastics, such as polyamides (up to 53.3%), polyethylene and polypropylene (up to 17.1%), and natural fragments such as cellulose (up to 45.8%) and wool (up 18.8%) in the same size range.
31628537	4	6	dep	Fourier	680:686	arg1	transform					688:696	transform	688:696	transform infrared spectroscopy (FTIR) 2D imaging	688:736	MPs were analysed and identified by a non-destructive method using Fourier transform infrared spectroscopy (FTIR) 2D imaging.
31628537	1	7	from	occurrence	260:269	arg1	environments					285:296	freshwater environments	274:296	freshwater environments	274:296	In the last few years, several studies have investigated microplastics (MPs) in marine ecosystems, but data monitoring and assessing the occurrence in freshwater environments are still scarce.
31628537	5	8	theme	MPs	766:768	arg1	MPs/kg					832:837	395.5 ± 90.7 MPs/kg	819:837	395.5 ± 90.7 MPs/kg	819:837	The mean concentrations of MPs detected in sediment, snow, and ice samples were 395.5 ± 90.7 MPs/kg, 117.1 ± 18.4 MPs/L, and 7.8 ± 1.2 MPs/L, respectively.
31628537	5	8	theme	MPs	766:768	arg1	concentrations					748:761	The mean concentrations	739:761	The mean concentrations of MPs detected in sediment, snow, and ice samples	739:812	The mean concentrations of MPs detected in sediment, snow, and ice samples were 395.5 ± 90.7 MPs/kg, 117.1 ± 18.4 MPs/L, and 7.8 ± 1.2 MPs/L, respectively.
31628537	1	9	theme	several	146:152	arg1	studies					154:160	several studies	146:160	several studies	146:160	In the last few years, several studies have investigated microplastics (MPs) in marine ecosystems, but data monitoring and assessing the occurrence in freshwater environments are still scarce.
31628537	6	10	theme	same	1120:1123	arg1	range					1130:1134	the same size range	1116:1134	the same size range	1116:1134	FTIR results showed the predominant abundance of microplastics, such as polyamides (up to 53.3%), polyethylene and polypropylene (up to 17.1%), and natural fragments such as cellulose (up to 45.8%) and wool (up 18.8%) in the same size range.
31628537	0	11	from	occurrence	38:47	arg1	lake					83:86	lake	83:86	lake	83:86	Assessment of microplastic pollution: occurrence and characterisation in Vesijärvi lake and Pikku Vesijärvi pond, Finland.
31628537	0	11	from	occurrence	38:47	arg1	Vesijärvi					98:106	Vesijärvi	98:106	Vesijärvi	98:106	Assessment of microplastic pollution: occurrence and characterisation in Vesijärvi lake and Pikku Vesijärvi pond, Finland.
31628537	6	12	theme	FTIR	895:898	arg1	results					900:906	FTIR results	895:906	FTIR results	895:906	FTIR results showed the predominant abundance of microplastics, such as polyamides (up to 53.3%), polyethylene and polypropylene (up to 17.1%), and natural fragments such as cellulose (up to 45.8%) and wool (up 18.8%) in the same size range.
31628537	3	13	theme	Sediment	516:523	arg1	samples					545:551	Sediment, snow, and ice core samples	516:551	Sediment, snow, and ice core samples	516:551	Sediment, snow, and ice core samples were collected near the shore of these two aquatic systems.
31628537	6	14	theme	microplastics	944:956	arg1	abundance					931:939	the predominant abundance	915:939	the predominant abundance of microplastics, such as polyamides (up to 53.3%), polyethylene and polypropylene (up to 17.1%), and natural fragments such as cellulose (up to 45.8%) and wool (up 18.8%) in the same size range	915:1134	FTIR results showed the predominant abundance of microplastics, such as polyamides (up to 53.3%), polyethylene and polypropylene (up to 17.1%), and natural fragments such as cellulose (up to 45.8%) and wool (up 18.8%) in the same size range.
31628537	2	15	theme	pollution	415:423	arg1	distribution					370:381	distribution	370:381	distribution	370:381	The present study aims to investigate the occurrence, distribution, and chemical composition of MP pollution in Vesijärvi lake and Pikku Vesijärvi pond close to the city of Lahti (Finland) in winter.
31628537	2	15	theme	pollution	415:423	arg1	occurrence					358:367	occurrence	358:367	occurrence	358:367	The present study aims to investigate the occurrence, distribution, and chemical composition of MP pollution in Vesijärvi lake and Pikku Vesijärvi pond close to the city of Lahti (Finland) in winter.
31628537	2	15	theme	pollution	415:423	arg1	composition					397:407	chemical composition	388:407	chemical composition	388:407	The present study aims to investigate the occurrence, distribution, and chemical composition of MP pollution in Vesijärvi lake and Pikku Vesijärvi pond close to the city of Lahti (Finland) in winter.
31628537	5	16	located	detected	770:777	arg1	samples					806:812	ice samples	802:812	ice samples	802:812	The mean concentrations of MPs detected in sediment, snow, and ice samples were 395.5 ± 90.7 MPs/kg, 117.1 ± 18.4 MPs/L, and 7.8 ± 1.2 MPs/L, respectively.
31628537	5	16	located	detected	770:777	arg1	snow					792:795	snow	792:795	snow	792:795	The mean concentrations of MPs detected in sediment, snow, and ice samples were 395.5 ± 90.7 MPs/kg, 117.1 ± 18.4 MPs/L, and 7.8 ± 1.2 MPs/L, respectively.
31628537	5	16	located	detected	770:777	arg2	MPs					766:768	MPs	766:768	MPs detected in sediment, snow, and ice samples	766:812	The mean concentrations of MPs detected in sediment, snow, and ice samples were 395.5 ± 90.7 MPs/kg, 117.1 ± 18.4 MPs/L, and 7.8 ± 1.2 MPs/L, respectively.
31628537	5	16	located	detected	770:777	arg1	sediment					782:789	sediment	782:789	sediment	782:789	The mean concentrations of MPs detected in sediment, snow, and ice samples were 395.5 ± 90.7 MPs/kg, 117.1 ± 18.4 MPs/L, and 7.8 ± 1.2 MPs/L, respectively.
31628537	6	17	from	abundance	931:939	arg1	range					1130:1134	the same size range	1116:1134	the same size range	1116:1134	FTIR results showed the predominant abundance of microplastics, such as polyamides (up to 53.3%), polyethylene and polypropylene (up to 17.1%), and natural fragments such as cellulose (up to 45.8%) and wool (up 18.8%) in the same size range.
31628537	0	18	dep	lake	83:86	arg1	Finland					114:120	Finland	114:120	Finland	114:120	Assessment of microplastic pollution: occurrence and characterisation in Vesijärvi lake and Pikku Vesijärvi pond, Finland.
31628537	0	19	theme	pollution	27:35	arg1	Assessment					0:9	Assessment	0:9	Assessment of microplastic pollution: occurrence and characterisation in Vesijärvi lake and Pikku Vesijärvi pond, Finland.	0:121	Assessment of microplastic pollution: occurrence and characterisation in Vesijärvi lake and Pikku Vesijärvi pond, Finland.
31628537	4	20	theme	non-destructive	651:665	arg1	method					667:672	a non-destructive method	649:672	a non-destructive method using Fourier transform infrared spectroscopy (FTIR) 2D imaging	649:736	MPs were analysed and identified by a non-destructive method using Fourier transform infrared spectroscopy (FTIR) 2D imaging.
31628537	7	21	theme	potential	1141:1149	arg1	release					1151:1157	The potential release	1137:1157	The potential release of MPs arising from stormwaters and sport and recreational activities	1137:1227	The potential release of MPs arising from stormwaters and sport and recreational activities was evidenced.
31628537	5	22	theme	±	846:846	arg1	MPs/L					853:857	117.1 ± 18.4 MPs/L	840:857	117.1 ± 18.4 MPs/L	840:857	The mean concentrations of MPs detected in sediment, snow, and ice samples were 395.5 ± 90.7 MPs/kg, 117.1 ± 18.4 MPs/L, and 7.8 ± 1.2 MPs/L, respectively.
31628537	0	23	theme	microplastic	14:25	arg1	pollution					27:35	microplastic pollution	14:35	microplastic pollution	14:35	Assessment of microplastic pollution: occurrence and characterisation in Vesijärvi lake and Pikku Vesijärvi pond, Finland.
31628537	6	24	dep	53.3	985:988	arg1	to					982:983	to	982:983	to	982:983	FTIR results showed the predominant abundance of microplastics, such as polyamides (up to 53.3%), polyethylene and polypropylene (up to 17.1%), and natural fragments such as cellulose (up to 45.8%) and wool (up 18.8%) in the same size range.
31628537	2	25	theme	chemical	388:395	arg1	composition					397:407	chemical composition	388:407	chemical composition	388:407	The present study aims to investigate the occurrence, distribution, and chemical composition of MP pollution in Vesijärvi lake and Pikku Vesijärvi pond close to the city of Lahti (Finland) in winter.
31628537	2	26	theme	Finland	496:502	arg1	city					481:484	the city	477:484	the city of Lahti (Finland) in winter	477:513	The present study aims to investigate the occurrence, distribution, and chemical composition of MP pollution in Vesijärvi lake and Pikku Vesijärvi pond close to the city of Lahti (Finland) in winter.
31628537	6	27	dep	17.1	1031:1034	arg1	to					1028:1029	to	1028:1029	to	1028:1029	FTIR results showed the predominant abundance of microplastics, such as polyamides (up to 53.3%), polyethylene and polypropylene (up to 17.1%), and natural fragments such as cellulose (up to 45.8%) and wool (up 18.8%) in the same size range.
31628537	1	28	theme	freshwater	274:283	arg1	environments					285:296	freshwater environments	274:296	freshwater environments	274:296	In the last few years, several studies have investigated microplastics (MPs) in marine ecosystems, but data monitoring and assessing the occurrence in freshwater environments are still scarce.
31628537	2	29	theme	MP	412:413	arg1	pollution					415:423	MP pollution	412:423	MP pollution	412:423	The present study aims to investigate the occurrence, distribution, and chemical composition of MP pollution in Vesijärvi lake and Pikku Vesijärvi pond close to the city of Lahti (Finland) in winter.
31628537	2	30	from	occurrence	358:367	arg1	lake					438:441	lake	438:441	lake	438:441	The present study aims to investigate the occurrence, distribution, and chemical composition of MP pollution in Vesijärvi lake and Pikku Vesijärvi pond close to the city of Lahti (Finland) in winter.
31628537	2	30	from	occurrence	358:367	arg1	Vesijärvi					453:461	Vesijärvi	453:461	Vesijärvi	453:461	The present study aims to investigate the occurrence, distribution, and chemical composition of MP pollution in Vesijärvi lake and Pikku Vesijärvi pond close to the city of Lahti (Finland) in winter.
31628537	5	31	theme	mean	743:746	arg1	MPs/kg					832:837	395.5 ± 90.7 MPs/kg	819:837	395.5 ± 90.7 MPs/kg	819:837	The mean concentrations of MPs detected in sediment, snow, and ice samples were 395.5 ± 90.7 MPs/kg, 117.1 ± 18.4 MPs/L, and 7.8 ± 1.2 MPs/L, respectively.
31628537	5	31	theme	mean	743:746	arg1	concentrations					748:761	The mean concentrations	739:761	The mean concentrations of MPs detected in sediment, snow, and ice samples	739:812	The mean concentrations of MPs detected in sediment, snow, and ice samples were 395.5 ± 90.7 MPs/kg, 117.1 ± 18.4 MPs/L, and 7.8 ± 1.2 MPs/L, respectively.
31628537	3	32	theme	snow	526:529	arg1	samples					545:551	Sediment, snow, and ice core samples	516:551	Sediment, snow, and ice core samples	516:551	Sediment, snow, and ice core samples were collected near the shore of these two aquatic systems.
31628537	2	33	theme	present	320:326	arg1	study					328:332	The present study	316:332	The present study	316:332	The present study aims to investigate the occurrence, distribution, and chemical composition of MP pollution in Vesijärvi lake and Pikku Vesijärvi pond close to the city of Lahti (Finland) in winter.
31628537	2	34	from	city	481:484	arg1	winter					508:513	winter	508:513	winter	508:513	The present study aims to investigate the occurrence, distribution, and chemical composition of MP pollution in Vesijärvi lake and Pikku Vesijärvi pond close to the city of Lahti (Finland) in winter.
31628537	6	35	theme	natural	1043:1049	arg1	fragments					1051:1059	natural fragments	1043:1059	natural fragments	1043:1059	FTIR results showed the predominant abundance of microplastics, such as polyamides (up to 53.3%), polyethylene and polypropylene (up to 17.1%), and natural fragments such as cellulose (up to 45.8%) and wool (up 18.8%) in the same size range.
31628537	6	35	theme	natural	1043:1049	arg1	cellulose					1069:1077	cellulose	1069:1077	cellulose (up to 45.8%)	1069:1091	FTIR results showed the predominant abundance of microplastics, such as polyamides (up to 53.3%), polyethylene and polypropylene (up to 17.1%), and natural fragments such as cellulose (up to 45.8%) and wool (up 18.8%) in the same size range.
31628537	6	35	theme	natural	1043:1049	arg1	wool					1097:1100	wool	1097:1100	wool (up 18.8%)	1097:1111	FTIR results showed the predominant abundance of microplastics, such as polyamides (up to 53.3%), polyethylene and polypropylene (up to 17.1%), and natural fragments such as cellulose (up to 45.8%) and wool (up 18.8%) in the same size range.
31628537	2	36	from	distribution	370:381	arg1	lake					438:441	lake	438:441	lake	438:441	The present study aims to investigate the occurrence, distribution, and chemical composition of MP pollution in Vesijärvi lake and Pikku Vesijärvi pond close to the city of Lahti (Finland) in winter.
31628537	2	36	from	distribution	370:381	arg1	Vesijärvi					453:461	Vesijärvi	453:461	Vesijärvi	453:461	The present study aims to investigate the occurrence, distribution, and chemical composition of MP pollution in Vesijärvi lake and Pikku Vesijärvi pond close to the city of Lahti (Finland) in winter.
31628537	5	37	theme	±	825:825	arg1	concentrations					748:761	The mean concentrations	739:761	The mean concentrations of MPs detected in sediment, snow, and ice samples	739:812	The mean concentrations of MPs detected in sediment, snow, and ice samples were 395.5 ± 90.7 MPs/kg, 117.1 ± 18.4 MPs/L, and 7.8 ± 1.2 MPs/L, respectively.
31628537	5	37	theme	±	825:825	arg1	MPs/kg					832:837	395.5 ± 90.7 MPs/kg	819:837	395.5 ± 90.7 MPs/kg	819:837	The mean concentrations of MPs detected in sediment, snow, and ice samples were 395.5 ± 90.7 MPs/kg, 117.1 ± 18.4 MPs/L, and 7.8 ± 1.2 MPs/L, respectively.
31628537	3	38	theme	systems	604:610	arg1	shore					577:581	the shore	573:581	the shore of these two aquatic systems	573:610	Sediment, snow, and ice core samples were collected near the shore of these two aquatic systems.
31628537	5	39	theme	±	868:868	arg1	MPs/L					874:878	7.8 ± 1.2 MPs/L	864:878	7.8 ± 1.2 MPs/L	864:878	The mean concentrations of MPs detected in sediment, snow, and ice samples were 395.5 ± 90.7 MPs/kg, 117.1 ± 18.4 MPs/L, and 7.8 ± 1.2 MPs/L, respectively.
31628537	3	40	theme	ice	536:538	arg1	samples					545:551	Sediment, snow, and ice core samples	516:551	Sediment, snow, and ice core samples	516:551	Sediment, snow, and ice core samples were collected near the shore of these two aquatic systems.
31628537	7	41	theme	MPs	1162:1164	arg1	release					1151:1157	The potential release	1137:1157	The potential release of MPs arising from stormwaters and sport and recreational activities	1137:1227	The potential release of MPs arising from stormwaters and sport and recreational activities was evidenced.
31628537	5	42	theme	ice	802:804	arg1	samples					806:812	ice samples	802:812	ice samples	802:812	The mean concentrations of MPs detected in sediment, snow, and ice samples were 395.5 ± 90.7 MPs/kg, 117.1 ± 18.4 MPs/L, and 7.8 ± 1.2 MPs/L, respectively.
31628537	1	43	theme	marine	203:208	arg1	ecosystems					210:219	marine ecosystems	203:219	marine ecosystems	203:219	In the last few years, several studies have investigated microplastics (MPs) in marine ecosystems, but data monitoring and assessing the occurrence in freshwater environments are still scarce.
31628537	1	44	dep	data	226:229	arg1	assessing					246:254	assessing	246:254	assessing the occurrence in freshwater environments	246:296	In the last few years, several studies have investigated microplastics (MPs) in marine ecosystems, but data monitoring and assessing the occurrence in freshwater environments are still scarce.
31628537	1	44	dep	data	226:229	arg1	monitoring					231:240	monitoring	231:240	monitoring	231:240	In the last few years, several studies have investigated microplastics (MPs) in marine ecosystems, but data monitoring and assessing the occurrence in freshwater environments are still scarce.
31628537	3	45	theme	core	540:543	arg1	samples					545:551	Sediment, snow, and ice core samples	516:551	Sediment, snow, and ice core samples	516:551	Sediment, snow, and ice core samples were collected near the shore of these two aquatic systems.
31628537	1	46	theme	last	130:133	arg1	years					139:143	the last few years	126:143	the last few years	126:143	In the last few years, several studies have investigated microplastics (MPs) in marine ecosystems, but data monitoring and assessing the occurrence in freshwater environments are still scarce.
31628537	3	47	theme	aquatic	596:602	arg1	systems					604:610	these two aquatic systems	586:610	these two aquatic systems	586:610	Sediment, snow, and ice core samples were collected near the shore of these two aquatic systems.
31628537	0	48	dep	Assessment	0:9	arg1	occurrence					38:47	occurrence	38:47	occurrence	38:47	Assessment of microplastic pollution: occurrence and characterisation in Vesijärvi lake and Pikku Vesijärvi pond, Finland.
31628537	0	48	dep	Assessment	0:9	arg1	characterisation					53:68	characterisation	53:68	characterisation	53:68	Assessment of microplastic pollution: occurrence and characterisation in Vesijärvi lake and Pikku Vesijärvi pond, Finland.
31628537	2	49	from	composition	397:407	arg1	lake					438:441	lake	438:441	lake	438:441	The present study aims to investigate the occurrence, distribution, and chemical composition of MP pollution in Vesijärvi lake and Pikku Vesijärvi pond close to the city of Lahti (Finland) in winter.
31628537	2	49	from	composition	397:407	arg1	Vesijärvi					453:461	Vesijärvi	453:461	Vesijärvi	453:461	The present study aims to investigate the occurrence, distribution, and chemical composition of MP pollution in Vesijärvi lake and Pikku Vesijärvi pond close to the city of Lahti (Finland) in winter.
31628537	1	50	theme	few	135:137	arg1	years					139:143	the last few years	126:143	the last few years	126:143	In the last few years, several studies have investigated microplastics (MPs) in marine ecosystems, but data monitoring and assessing the occurrence in freshwater environments are still scarce.
30424538	0	0	theme	Independent	98:108	arg1	Fatigue					90:96	Post-Exercise Fatigue	76:96	Post-Exercise Fatigue Independent of Gut Microbiota	76:126	Supplementation with Beef Extract Improves Exercise Performance and Reduces Post-Exercise Fatigue Independent of Gut Microbiota.
30424538	6	1	theme	dose-dependent	947:960	arg1	manner					962:967	a dose-dependent manner	945:967	a dose-dependent manner	945:967	BE supplementation elevated endurance and grip strength in a dose-dependent manner; significantly decreased lactate and blood urea nitrogen levels after physical challenge; and significantly increased muscle glycogen content.
30424538	3	2	theme	exercise	448:455	arg1	performance					457:467	exercise performance	448:467	exercise performance	448:467	In this study, we evaluated the potential beneficial effects of BE on exercise performance and the related role of the gut microbiota.
30424538	3	3	from	role	485:488	arg1	performance					457:467	exercise performance	448:467	exercise performance	448:467	In this study, we evaluated the potential beneficial effects of BE on exercise performance and the related role of the gut microbiota.
30424538	4	4	theme	BALB/c	532:537	arg1	mice					539:542	Pathogen-free male BALB/c mice	513:542	Pathogen-free male BALB/c mice	513:542	Pathogen-free male BALB/c mice were divided into three groups to receive vehicle or BE (0, 12.3, or 24.6 mL/kg) by oral gavage for 28 days.
30424538	8	5	with	correlation	1431:1441	arg1	microbiota					1456:1465	the gut microbiota	1448:1465	the gut microbiota	1448:1465	Therefore, BE supplementation improved endurance and reduced fatigue, which might be related to BE composition, but had no correlation with the gut microbiota.
30424538	1	6	theme	nutritional	152:162	arg1	supplement					164:173	a nutritional supplement	150:173	a nutritional supplement obtained by cooking beef meat	150:203	Beef extract (BE) is a nutritional supplement obtained by cooking beef meat.
30424538	1	6	theme	nutritional	152:162	arg1	extract					134:140	Beef extract	129:140	Beef extract (BE)	129:145	Beef extract (BE) is a nutritional supplement obtained by cooking beef meat.
30424538	4	7	theme	male	527:530	arg1	mice					539:542	Pathogen-free male BALB/c mice	513:542	Pathogen-free male BALB/c mice	513:542	Pathogen-free male BALB/c mice were divided into three groups to receive vehicle or BE (0, 12.3, or 24.6 mL/kg) by oral gavage for 28 days.
30424538	0	8	theme	Gut	113:115	arg1	Microbiota					117:126	Gut Microbiota	113:126	Gut Microbiota	113:126	Supplementation with Beef Extract Improves Exercise Performance and Reduces Post-Exercise Fatigue Independent of Gut Microbiota.
30424538	6	9	dep	supplementation	889:903	arg1	BE					886:887	BE	886:887	BE	886:887	BE supplementation elevated endurance and grip strength in a dose-dependent manner; significantly decreased lactate and blood urea nitrogen levels after physical challenge; and significantly increased muscle glycogen content.
30424538	7	10	theme	biomarkers	1296:1305	arg1	levels					1278:1283	levels	1278:1283	levels of related biomarkers	1278:1305	The germ-free mice supplemented with BE or an equal-calorie portion of albumin did not show significant differences from the other groups in exercise performance and levels of related biomarkers.
30424538	7	10	theme	biomarkers	1296:1305	arg1	performance					1262:1272	exercise performance	1253:1272	exercise performance	1253:1272	The germ-free mice supplemented with BE or an equal-calorie portion of albumin did not show significant differences from the other groups in exercise performance and levels of related biomarkers.
30424538	7	11	theme	other	1237:1241	arg1	groups					1243:1248	the other groups	1233:1248	the other groups	1233:1248	The germ-free mice supplemented with BE or an equal-calorie portion of albumin did not show significant differences from the other groups in exercise performance and levels of related biomarkers.
30424538	3	12	theme	microbiota	501:510	arg1	effects					431:437	the potential beneficial effects	406:437	the potential beneficial effects of BE on exercise performance	406:467	In this study, we evaluated the potential beneficial effects of BE on exercise performance and the related role of the gut microbiota.
30424538	3	12	theme	microbiota	501:510	arg1	role					485:488	the related role	473:488	the related role of the gut microbiota	473:510	In this study, we evaluated the potential beneficial effects of BE on exercise performance and the related role of the gut microbiota.
30424538	5	13	theme	serum	803:807	arg1	lactate					809:815	serum lactate	803:815	serum lactate	803:815	Exercise performance was evaluated using forelimb grip strength, swimming time to exhaustion, and physiological levels of fatigue-related biomarkers (serum lactate, blood urea nitrogen, and glucose levels) after physical challenges.
30424538	3	14	theme	BE	442:443	arg1	effects					431:437	the potential beneficial effects	406:437	the potential beneficial effects of BE on exercise performance	406:467	In this study, we evaluated the potential beneficial effects of BE on exercise performance and the related role of the gut microbiota.
30424538	3	14	theme	BE	442:443	arg1	role					485:488	the related role	473:488	the related role of the gut microbiota	473:510	In this study, we evaluated the potential beneficial effects of BE on exercise performance and the related role of the gut microbiota.
30424538	6	15	theme	blood	1006:1010	arg1	nitrogen					1017:1024	blood urea nitrogen	1006:1024	blood urea nitrogen	1006:1024	BE supplementation elevated endurance and grip strength in a dose-dependent manner; significantly decreased lactate and blood urea nitrogen levels after physical challenge; and significantly increased muscle glycogen content.
30424538	3	16	theme	related	477:483	arg1	role					485:488	the related role	473:488	the related role of the gut microbiota	473:510	In this study, we evaluated the potential beneficial effects of BE on exercise performance and the related role of the gut microbiota.
30424538	5	17	theme	physiological	751:763	arg1	levels					765:770	physiological levels	751:770	physiological levels of fatigue-related biomarkers (serum lactate, blood urea nitrogen, and glucose levels)	751:857	Exercise performance was evaluated using forelimb grip strength, swimming time to exhaustion, and physiological levels of fatigue-related biomarkers (serum lactate, blood urea nitrogen, and glucose levels) after physical challenges.
30424538	0	18	theme	Microbiota	117:126	arg1	Independent					98:108	Independent	98:108	Independent	98:108	Supplementation with Beef Extract Improves Exercise Performance and Reduces Post-Exercise Fatigue Independent of Gut Microbiota.
30424538	5	19	theme	lactate	809:815	arg1	biomarkers					791:800	fatigue-related biomarkers	775:800	fatigue-related biomarkers (serum lactate, blood urea nitrogen, and glucose levels)	775:857	Exercise performance was evaluated using forelimb grip strength, swimming time to exhaustion, and physiological levels of fatigue-related biomarkers (serum lactate, blood urea nitrogen, and glucose levels) after physical challenges.
30424538	5	19	theme	lactate	809:815	arg1	levels					851:856	serum lactate, blood urea nitrogen, and glucose levels	803:856	serum lactate, blood urea nitrogen, and glucose levels	803:856	Exercise performance was evaluated using forelimb grip strength, swimming time to exhaustion, and physiological levels of fatigue-related biomarkers (serum lactate, blood urea nitrogen, and glucose levels) after physical challenges.
30424538	2	20	used	used	301:304	arg2	BE					265:266	BE	265:266	BE	265:266	Compared with traditional chicken essence or clam extract, BE is cheaper to produce and may be used for wound healing, as a chemotherapy supplement, or to prevent fatigue.
30424538	1	21	dep	extract	134:140	arg1	BE					143:144	BE	143:144	BE	143:144	Beef extract (BE) is a nutritional supplement obtained by cooking beef meat.
30424538	8	22	theme	gut	1452:1454	arg1	microbiota					1456:1465	the gut microbiota	1448:1465	the gut microbiota	1448:1465	Therefore, BE supplementation improved endurance and reduced fatigue, which might be related to BE composition, but had no correlation with the gut microbiota.
30424538	6	23	theme	increased	1077:1085	arg1	content					1103:1109	significantly increased muscle glycogen content	1063:1109	significantly increased muscle glycogen content	1063:1109	BE supplementation elevated endurance and grip strength in a dose-dependent manner; significantly decreased lactate and blood urea nitrogen levels after physical challenge; and significantly increased muscle glycogen content.
30424538	6	24	theme	grip	928:931	arg1	strength					933:940	grip strength	928:940	grip strength	928:940	BE supplementation elevated endurance and grip strength in a dose-dependent manner; significantly decreased lactate and blood urea nitrogen levels after physical challenge; and significantly increased muscle glycogen content.
30424538	5	25	theme	fatigue-related	775:789	arg1	biomarkers					791:800	fatigue-related biomarkers	775:800	fatigue-related biomarkers (serum lactate, blood urea nitrogen, and glucose levels)	775:857	Exercise performance was evaluated using forelimb grip strength, swimming time to exhaustion, and physiological levels of fatigue-related biomarkers (serum lactate, blood urea nitrogen, and glucose levels) after physical challenges.
30424538	5	25	theme	fatigue-related	775:789	arg1	levels					851:856	serum lactate, blood urea nitrogen, and glucose levels	803:856	serum lactate, blood urea nitrogen, and glucose levels	803:856	Exercise performance was evaluated using forelimb grip strength, swimming time to exhaustion, and physiological levels of fatigue-related biomarkers (serum lactate, blood urea nitrogen, and glucose levels) after physical challenges.
30424538	5	26	theme	swimming	718:725	arg1	time					727:730	swimming time	718:730	swimming time to exhaustion	718:744	Exercise performance was evaluated using forelimb grip strength, swimming time to exhaustion, and physiological levels of fatigue-related biomarkers (serum lactate, blood urea nitrogen, and glucose levels) after physical challenges.
30424538	8	27	dep	supplementation	1322:1336	arg1	BE					1319:1320	BE	1319:1320	BE	1319:1320	Therefore, BE supplementation improved endurance and reduced fatigue, which might be related to BE composition, but had no correlation with the gut microbiota.
30424538	3	28	theme	gut	497:499	arg1	microbiota					501:510	the gut microbiota	493:510	the gut microbiota	493:510	In this study, we evaluated the potential beneficial effects of BE on exercise performance and the related role of the gut microbiota.
30424538	1	29	theme	beef	195:198	arg1	meat					200:203	beef meat	195:203	beef meat	195:203	Beef extract (BE) is a nutritional supplement obtained by cooking beef meat.
30424538	0	30	theme	Beef	21:24	arg1	Extract					26:32	Beef Extract	21:32	Beef Extract	21:32	Supplementation with Beef Extract Improves Exercise Performance and Reduces Post-Exercise Fatigue Independent of Gut Microbiota.
30424538	4	31	theme	Pathogen-free	513:525	arg1	mice					539:542	Pathogen-free male BALB/c mice	513:542	Pathogen-free male BALB/c mice	513:542	Pathogen-free male BALB/c mice were divided into three groups to receive vehicle or BE (0, 12.3, or 24.6 mL/kg) by oral gavage for 28 days.
30424538	5	32	theme	physical	865:872	arg1	challenges					874:883	physical challenges	865:883	physical challenges	865:883	Exercise performance was evaluated using forelimb grip strength, swimming time to exhaustion, and physiological levels of fatigue-related biomarkers (serum lactate, blood urea nitrogen, and glucose levels) after physical challenges.
30424538	2	33	dep	cheaper	271:277	arg1	used					301:304	used	301:304	used	301:304	Compared with traditional chicken essence or clam extract, BE is cheaper to produce and may be used for wound healing, as a chemotherapy supplement, or to prevent fatigue.
30424538	2	33	dep	cheaper	271:277	arg1	prevent					361:367	prevent	361:367	to prevent fatigue	358:375	Compared with traditional chicken essence or clam extract, BE is cheaper to produce and may be used for wound healing, as a chemotherapy supplement, or to prevent fatigue.
30424538	2	33	dep	cheaper	271:277	arg1	produce					282:288	produce	282:288	produce	282:288	Compared with traditional chicken essence or clam extract, BE is cheaper to produce and may be used for wound healing, as a chemotherapy supplement, or to prevent fatigue.
30424538	3	34	theme	beneficial	420:429	arg1	effects					431:437	the potential beneficial effects	406:437	the potential beneficial effects of BE on exercise performance	406:467	In this study, we evaluated the potential beneficial effects of BE on exercise performance and the related role of the gut microbiota.
30424538	0	35	theme	Exercise	43:50	arg1	Performance					52:62	Exercise Performance	43:62	Exercise Performance	43:62	Supplementation with Beef Extract Improves Exercise Performance and Reduces Post-Exercise Fatigue Independent of Gut Microbiota.
30424538	5	36	theme	blood	818:822	arg1	nitrogen					829:836	blood urea nitrogen	818:836	blood urea nitrogen	818:836	Exercise performance was evaluated using forelimb grip strength, swimming time to exhaustion, and physiological levels of fatigue-related biomarkers (serum lactate, blood urea nitrogen, and glucose levels) after physical challenges.
30424538	6	37	theme	physical	1039:1046	arg1	challenge					1048:1056	physical challenge	1039:1056	physical challenge	1039:1056	BE supplementation elevated endurance and grip strength in a dose-dependent manner; significantly decreased lactate and blood urea nitrogen levels after physical challenge; and significantly increased muscle glycogen content.
30424538	8	38	contain	had	1424:1426	arg2	correlation					1431:1441	no correlation	1428:1441	no correlation with the gut microbiota	1428:1465	Therefore, BE supplementation improved endurance and reduced fatigue, which might be related to BE composition, but had no correlation with the gut microbiota.
30424538	8	38	contain	had	1424:1426	arg1	supplementation					1322:1336	BE supplementation	1319:1336	BE supplementation	1319:1336	Therefore, BE supplementation improved endurance and reduced fatigue, which might be related to BE composition, but had no correlation with the gut microbiota.
30424538	6	39	theme	lactate	994:1000	arg1	levels					1026:1031	lactate and blood urea nitrogen levels	994:1031	lactate and blood urea nitrogen levels	994:1031	BE supplementation elevated endurance and grip strength in a dose-dependent manner; significantly decreased lactate and blood urea nitrogen levels after physical challenge; and significantly increased muscle glycogen content.
30424538	7	40	theme	exercise	1253:1260	arg1	performance					1262:1272	exercise performance	1253:1272	exercise performance	1253:1272	The germ-free mice supplemented with BE or an equal-calorie portion of albumin did not show significant differences from the other groups in exercise performance and levels of related biomarkers.
30424538	2	41	theme	chicken	232:238	arg1	essence					240:246	traditional chicken essence	220:246	traditional chicken essence	220:246	Compared with traditional chicken essence or clam extract, BE is cheaper to produce and may be used for wound healing, as a chemotherapy supplement, or to prevent fatigue.
30424538	7	42	from	differences	1216:1226	arg1	levels					1278:1283	levels	1278:1283	levels of related biomarkers	1278:1305	The germ-free mice supplemented with BE or an equal-calorie portion of albumin did not show significant differences from the other groups in exercise performance and levels of related biomarkers.
30424538	7	42	from	differences	1216:1226	arg1	performance					1262:1272	exercise performance	1253:1272	exercise performance	1253:1272	The germ-free mice supplemented with BE or an equal-calorie portion of albumin did not show significant differences from the other groups in exercise performance and levels of related biomarkers.
30424538	7	43	theme	germ-free	1116:1124	arg1	mice					1126:1129	The germ-free mice	1112:1129	The germ-free mice supplemented with BE or an equal-calorie portion of albumin	1112:1189	The germ-free mice supplemented with BE or an equal-calorie portion of albumin did not show significant differences from the other groups in exercise performance and levels of related biomarkers.
30424538	5	44	theme	forelimb	694:701	arg1	strength					708:715	forelimb grip strength	694:715	forelimb grip strength	694:715	Exercise performance was evaluated using forelimb grip strength, swimming time to exhaustion, and physiological levels of fatigue-related biomarkers (serum lactate, blood urea nitrogen, and glucose levels) after physical challenges.
30424538	5	45	theme	biomarkers	791:800	arg1	levels					765:770	physiological levels	751:770	physiological levels of fatigue-related biomarkers (serum lactate, blood urea nitrogen, and glucose levels)	751:857	Exercise performance was evaluated using forelimb grip strength, swimming time to exhaustion, and physiological levels of fatigue-related biomarkers (serum lactate, blood urea nitrogen, and glucose levels) after physical challenges.
30424538	5	45	theme	biomarkers	791:800	arg1	strength					708:715	forelimb grip strength	694:715	forelimb grip strength	694:715	Exercise performance was evaluated using forelimb grip strength, swimming time to exhaustion, and physiological levels of fatigue-related biomarkers (serum lactate, blood urea nitrogen, and glucose levels) after physical challenges.
30424538	5	45	theme	biomarkers	791:800	arg1	time					727:730	swimming time	718:730	swimming time to exhaustion	718:744	Exercise performance was evaluated using forelimb grip strength, swimming time to exhaustion, and physiological levels of fatigue-related biomarkers (serum lactate, blood urea nitrogen, and glucose levels) after physical challenges.
30424538	2	46	theme	traditional	220:230	arg1	essence					240:246	traditional chicken essence	220:246	traditional chicken essence	220:246	Compared with traditional chicken essence or clam extract, BE is cheaper to produce and may be used for wound healing, as a chemotherapy supplement, or to prevent fatigue.
30424538	6	47	theme	nitrogen	1017:1024	arg1	levels					1026:1031	lactate and blood urea nitrogen levels	994:1031	lactate and blood urea nitrogen levels	994:1031	BE supplementation elevated endurance and grip strength in a dose-dependent manner; significantly decreased lactate and blood urea nitrogen levels after physical challenge; and significantly increased muscle glycogen content.
30424538	1	48	theme	Beef	129:132	arg1	supplement					164:173	a nutritional supplement	150:173	a nutritional supplement obtained by cooking beef meat	150:203	Beef extract (BE) is a nutritional supplement obtained by cooking beef meat.
30424538	1	48	theme	Beef	129:132	arg1	extract					134:140	Beef extract	129:140	Beef extract (BE)	129:145	Beef extract (BE) is a nutritional supplement obtained by cooking beef meat.
30424538	5	49	theme	grip	703:706	arg1	strength					708:715	forelimb grip strength	694:715	forelimb grip strength	694:715	Exercise performance was evaluated using forelimb grip strength, swimming time to exhaustion, and physiological levels of fatigue-related biomarkers (serum lactate, blood urea nitrogen, and glucose levels) after physical challenges.
30424538	2	50	theme	wound	310:314	arg1	healing					316:322	wound healing	310:322	wound healing	310:322	Compared with traditional chicken essence or clam extract, BE is cheaper to produce and may be used for wound healing, as a chemotherapy supplement, or to prevent fatigue.
30424538	7	51	theme	albumin	1183:1189	arg1	portion					1172:1178	an equal-calorie portion	1155:1178	an equal-calorie portion of albumin	1155:1189	The germ-free mice supplemented with BE or an equal-calorie portion of albumin did not show significant differences from the other groups in exercise performance and levels of related biomarkers.
30424538	3	52	from	effects	431:437	arg1	performance					457:467	exercise performance	448:467	exercise performance	448:467	In this study, we evaluated the potential beneficial effects of BE on exercise performance and the related role of the gut microbiota.
30424538	0	53	with	Supplementation	0:14	arg1	Extract					26:32	Beef Extract	21:32	Beef Extract	21:32	Supplementation with Beef Extract Improves Exercise Performance and Reduces Post-Exercise Fatigue Independent of Gut Microbiota.
30424538	4	54	theme	oral	628:631	arg1	gavage					633:638	oral gavage	628:638	oral gavage for 28 days	628:650	Pathogen-free male BALB/c mice were divided into three groups to receive vehicle or BE (0, 12.3, or 24.6 mL/kg) by oral gavage for 28 days.
30424538	5	55	theme	glucose	843:849	arg1	biomarkers					791:800	fatigue-related biomarkers	775:800	fatigue-related biomarkers (serum lactate, blood urea nitrogen, and glucose levels)	775:857	Exercise performance was evaluated using forelimb grip strength, swimming time to exhaustion, and physiological levels of fatigue-related biomarkers (serum lactate, blood urea nitrogen, and glucose levels) after physical challenges.
30424538	5	55	theme	glucose	843:849	arg1	levels					851:856	serum lactate, blood urea nitrogen, and glucose levels	803:856	serum lactate, blood urea nitrogen, and glucose levels	803:856	Exercise performance was evaluated using forelimb grip strength, swimming time to exhaustion, and physiological levels of fatigue-related biomarkers (serum lactate, blood urea nitrogen, and glucose levels) after physical challenges.
30424538	6	56	theme	muscle	1087:1092	arg1	content					1103:1109	significantly increased muscle glycogen content	1063:1109	significantly increased muscle glycogen content	1063:1109	BE supplementation elevated endurance and grip strength in a dose-dependent manner; significantly decreased lactate and blood urea nitrogen levels after physical challenge; and significantly increased muscle glycogen content.
30424538	7	57	theme	related	1288:1294	arg1	biomarkers					1296:1305	related biomarkers	1288:1305	related biomarkers	1288:1305	The germ-free mice supplemented with BE or an equal-calorie portion of albumin did not show significant differences from the other groups in exercise performance and levels of related biomarkers.
30424538	7	58	from	groups	1243:1248	arg1	differences					1216:1226	significant differences	1204:1226	significant differences from the other groups in exercise performance and levels of related biomarkers	1204:1305	The germ-free mice supplemented with BE or an equal-calorie portion of albumin did not show significant differences from the other groups in exercise performance and levels of related biomarkers.
30424538	5	59	theme	nitrogen	829:836	arg1	biomarkers					791:800	fatigue-related biomarkers	775:800	fatigue-related biomarkers (serum lactate, blood urea nitrogen, and glucose levels)	775:857	Exercise performance was evaluated using forelimb grip strength, swimming time to exhaustion, and physiological levels of fatigue-related biomarkers (serum lactate, blood urea nitrogen, and glucose levels) after physical challenges.
30424538	5	59	theme	nitrogen	829:836	arg1	levels					851:856	serum lactate, blood urea nitrogen, and glucose levels	803:856	serum lactate, blood urea nitrogen, and glucose levels	803:856	Exercise performance was evaluated using forelimb grip strength, swimming time to exhaustion, and physiological levels of fatigue-related biomarkers (serum lactate, blood urea nitrogen, and glucose levels) after physical challenges.
30424538	5	60	theme	urea	824:827	arg1	nitrogen					829:836	blood urea nitrogen	818:836	blood urea nitrogen	818:836	Exercise performance was evaluated using forelimb grip strength, swimming time to exhaustion, and physiological levels of fatigue-related biomarkers (serum lactate, blood urea nitrogen, and glucose levels) after physical challenges.
30424538	0	61	theme	Post-Exercise	76:88	arg1	Fatigue					90:96	Post-Exercise Fatigue	76:96	Post-Exercise Fatigue Independent of Gut Microbiota	76:126	Supplementation with Beef Extract Improves Exercise Performance and Reduces Post-Exercise Fatigue Independent of Gut Microbiota.
30424538	2	62	theme	clam	251:254	arg1	extract					256:262	clam extract	251:262	clam extract	251:262	Compared with traditional chicken essence or clam extract, BE is cheaper to produce and may be used for wound healing, as a chemotherapy supplement, or to prevent fatigue.
30424538	3	63	theme	potential	410:418	arg1	effects					431:437	the potential beneficial effects	406:437	the potential beneficial effects of BE on exercise performance	406:467	In this study, we evaluated the potential beneficial effects of BE on exercise performance and the related role of the gut microbiota.
30424538	2	64	theme	chemotherapy	330:341	arg1	supplement					343:352	a chemotherapy supplement	328:352	a chemotherapy supplement	328:352	Compared with traditional chicken essence or clam extract, BE is cheaper to produce and may be used for wound healing, as a chemotherapy supplement, or to prevent fatigue.
30424538	6	65	theme	glycogen	1094:1101	arg1	content					1103:1109	significantly increased muscle glycogen content	1063:1109	significantly increased muscle glycogen content	1063:1109	BE supplementation elevated endurance and grip strength in a dose-dependent manner; significantly decreased lactate and blood urea nitrogen levels after physical challenge; and significantly increased muscle glycogen content.
30424538	5	66	theme	Exercise	653:660	arg1	performance					662:672	Exercise performance	653:672	Exercise performance	653:672	Exercise performance was evaluated using forelimb grip strength, swimming time to exhaustion, and physiological levels of fatigue-related biomarkers (serum lactate, blood urea nitrogen, and glucose levels) after physical challenges.
30424538	7	67	theme	significant	1204:1214	arg1	differences					1216:1226	significant differences	1204:1226	significant differences from the other groups in exercise performance and levels of related biomarkers	1204:1305	The germ-free mice supplemented with BE or an equal-calorie portion of albumin did not show significant differences from the other groups in exercise performance and levels of related biomarkers.
30424538	6	68	theme	urea	1012:1015	arg1	nitrogen					1017:1024	blood urea nitrogen	1006:1024	blood urea nitrogen	1006:1024	BE supplementation elevated endurance and grip strength in a dose-dependent manner; significantly decreased lactate and blood urea nitrogen levels after physical challenge; and significantly increased muscle glycogen content.
30424538	7	69	theme	equal-calorie	1158:1170	arg1	portion					1172:1178	an equal-calorie portion	1155:1178	an equal-calorie portion of albumin	1155:1189	The germ-free mice supplemented with BE or an equal-calorie portion of albumin did not show significant differences from the other groups in exercise performance and levels of related biomarkers.
30245270	0	0	theme	blaTEM-2	113:120	arg1	gene					122:125	blaTEM-2 gene	113:125	blaTEM-2 gene	113:125	Evaluation of antibiotic resistant lactose fermentative opportunistic pathogenic Enterobacteriaceae bacteria and blaTEM-2 gene in cephalosporin wastewater and its discharge receiving river.
30245270	4	1	theme	CPWWin	1220:1225	arg1	influent					1186:1193	the influent	1182:1193	the influent of CPWW treatment plant (CPWWin), CPWWeff and RWdown	1182:1246	The antibiotic resistant phenotypes of isolates in the influent of CPWW treatment plant (CPWWin), CPWWeff and RWdown appeared to be seriously affected by the cephalosporin residues, which suggested that main antibiotic resistance phenotypes in antibiotic contaminated water were closely associated with its antibiotic composition.
30245270	3	2	theme	LFOPEB	972:977	arg1	percentages					927:937	the percentages	923:937	the percentages of β-lactam antibiotic-resistant LFOPEB	923:977	Furthermore, the discharge of the effluent of CPWW treatment plant (CPWWeff) led to an obvious increase in the percentages of β-lactam antibiotic-resistant LFOPEB and relative abundance of the blaTEM-2 gene in the downstream receiving river (RWdown) in comparison with those in the upstream receiving river (RWup).
30245270	1	3	theme	opportunistic	295:307	arg1	LFOPEB					349:354	LFOPEB	349:354	LFOPEB	349:354	This study investigated the concentration of cephalosporin, the resistant levels of lactose fermentative opportunistic pathogenic Enterobacteriaceae bacteria (LFOPEB) against seven antibiotics and one cephalosporin-resistant gene in cephalosporin wastewater (CPWW) treatment plant and its discharge receiving river.
30245270	1	3	theme	opportunistic	295:307	arg1	bacteria					339:346	opportunistic pathogenic Enterobacteriaceae bacteria	295:346	opportunistic pathogenic Enterobacteriaceae bacteria (LFOPEB)	295:355	This study investigated the concentration of cephalosporin, the resistant levels of lactose fermentative opportunistic pathogenic Enterobacteriaceae bacteria (LFOPEB) against seven antibiotics and one cephalosporin-resistant gene in cephalosporin wastewater (CPWW) treatment plant and its discharge receiving river.
30245270	3	4	theme	obvious	903:909	arg1	increase					911:918	an obvious increase	900:918	an obvious increase in the percentages of β-lactam antibiotic-resistant LFOPEB	900:977	Furthermore, the discharge of the effluent of CPWW treatment plant (CPWWeff) led to an obvious increase in the percentages of β-lactam antibiotic-resistant LFOPEB and relative abundance of the blaTEM-2 gene in the downstream receiving river (RWdown) in comparison with those in the upstream receiving river (RWup).
30245270	4	5	theme	antibiotic	1438:1447	arg1	composition					1449:1459	its antibiotic composition	1434:1459	its antibiotic composition	1434:1459	The antibiotic resistant phenotypes of isolates in the influent of CPWW treatment plant (CPWWin), CPWWeff and RWdown appeared to be seriously affected by the cephalosporin residues, which suggested that main antibiotic resistance phenotypes in antibiotic contaminated water were closely associated with its antibiotic composition.
30245270	4	6	theme	antibiotic	1339:1348	arg1	phenotypes					1361:1370	main antibiotic resistance phenotypes	1334:1370	main antibiotic resistance phenotypes in antibiotic contaminated water	1334:1403	The antibiotic resistant phenotypes of isolates in the influent of CPWW treatment plant (CPWWin), CPWWeff and RWdown appeared to be seriously affected by the cephalosporin residues, which suggested that main antibiotic resistance phenotypes in antibiotic contaminated water were closely associated with its antibiotic composition.
30245270	1	7	theme	pathogenic	309:318	arg1	LFOPEB					349:354	LFOPEB	349:354	LFOPEB	349:354	This study investigated the concentration of cephalosporin, the resistant levels of lactose fermentative opportunistic pathogenic Enterobacteriaceae bacteria (LFOPEB) against seven antibiotics and one cephalosporin-resistant gene in cephalosporin wastewater (CPWW) treatment plant and its discharge receiving river.
30245270	1	7	theme	pathogenic	309:318	arg1	bacteria					339:346	opportunistic pathogenic Enterobacteriaceae bacteria	295:346	opportunistic pathogenic Enterobacteriaceae bacteria (LFOPEB)	295:355	This study investigated the concentration of cephalosporin, the resistant levels of lactose fermentative opportunistic pathogenic Enterobacteriaceae bacteria (LFOPEB) against seven antibiotics and one cephalosporin-resistant gene in cephalosporin wastewater (CPWW) treatment plant and its discharge receiving river.
30245270	2	8	theme	antibiotic	598:607	arg1	rates					619:623	the antibiotic resistant rates	594:623	the antibiotic resistant rates of the isolates to β-lactam antibiotics	594:663	Although large numbers of bacteria have been removed during the CPWW treatment process, the antibiotic resistant rates of the isolates to β-lactam antibiotics significantly increased (p = 0.032) after treatment, while the percentage of resistant LFOPEB to non-β-lactam antibiotics did not change dramatically.
30245270	1	9	theme	cephalosporin	423:435	arg1	plant					465:469	cephalosporin wastewater (CPWW) treatment plant	423:469	cephalosporin wastewater (CPWW) treatment plant	423:469	This study investigated the concentration of cephalosporin, the resistant levels of lactose fermentative opportunistic pathogenic Enterobacteriaceae bacteria (LFOPEB) against seven antibiotics and one cephalosporin-resistant gene in cephalosporin wastewater (CPWW) treatment plant and its discharge receiving river.
30245270	3	10	theme	β-lactam	942:949	arg1	LFOPEB					972:977	β-lactam antibiotic-resistant LFOPEB	942:977	β-lactam antibiotic-resistant LFOPEB	942:977	Furthermore, the discharge of the effluent of CPWW treatment plant (CPWWeff) led to an obvious increase in the percentages of β-lactam antibiotic-resistant LFOPEB and relative abundance of the blaTEM-2 gene in the downstream receiving river (RWdown) in comparison with those in the upstream receiving river (RWup).
30245270	1	11	theme	Enterobacteriaceae	320:337	arg1	LFOPEB					349:354	LFOPEB	349:354	LFOPEB	349:354	This study investigated the concentration of cephalosporin, the resistant levels of lactose fermentative opportunistic pathogenic Enterobacteriaceae bacteria (LFOPEB) against seven antibiotics and one cephalosporin-resistant gene in cephalosporin wastewater (CPWW) treatment plant and its discharge receiving river.
30245270	1	11	theme	Enterobacteriaceae	320:337	arg1	bacteria					339:346	opportunistic pathogenic Enterobacteriaceae bacteria	295:346	opportunistic pathogenic Enterobacteriaceae bacteria (LFOPEB)	295:355	This study investigated the concentration of cephalosporin, the resistant levels of lactose fermentative opportunistic pathogenic Enterobacteriaceae bacteria (LFOPEB) against seven antibiotics and one cephalosporin-resistant gene in cephalosporin wastewater (CPWW) treatment plant and its discharge receiving river.
30245270	1	12	theme	wastewater	437:446	arg1	plant					465:469	cephalosporin wastewater (CPWW) treatment plant	423:469	cephalosporin wastewater (CPWW) treatment plant	423:469	This study investigated the concentration of cephalosporin, the resistant levels of lactose fermentative opportunistic pathogenic Enterobacteriaceae bacteria (LFOPEB) against seven antibiotics and one cephalosporin-resistant gene in cephalosporin wastewater (CPWW) treatment plant and its discharge receiving river.
30245270	1	13	from	antibiotics	371:381	arg1	plant					465:469	cephalosporin wastewater (CPWW) treatment plant	423:469	cephalosporin wastewater (CPWW) treatment plant	423:469	This study investigated the concentration of cephalosporin, the resistant levels of lactose fermentative opportunistic pathogenic Enterobacteriaceae bacteria (LFOPEB) against seven antibiotics and one cephalosporin-resistant gene in cephalosporin wastewater (CPWW) treatment plant and its discharge receiving river.
30245270	1	13	from	antibiotics	371:381	arg1	discharge					479:487	its discharge	475:487	its discharge receiving river	475:503	This study investigated the concentration of cephalosporin, the resistant levels of lactose fermentative opportunistic pathogenic Enterobacteriaceae bacteria (LFOPEB) against seven antibiotics and one cephalosporin-resistant gene in cephalosporin wastewater (CPWW) treatment plant and its discharge receiving river.
30245270	4	14	theme	cephalosporin	1289:1301	arg1	residues					1303:1310	the cephalosporin residues	1285:1310	the cephalosporin residues	1285:1310	The antibiotic resistant phenotypes of isolates in the influent of CPWW treatment plant (CPWWin), CPWWeff and RWdown appeared to be seriously affected by the cephalosporin residues, which suggested that main antibiotic resistance phenotypes in antibiotic contaminated water were closely associated with its antibiotic composition.
30245270	5	15	theme	selective	1525:1533	arg1	growth					1535:1540	selective growth	1525:1540	selective growth of ARB	1525:1547	Therefore, CPWW treatment process has been proved to result in selective growth of ARB and proliferation of ARG.
30245270	0	16	theme	cephalosporin	130:142	arg1	wastewater					144:153	cephalosporin wastewater	130:153	cephalosporin wastewater	130:153	Evaluation of antibiotic resistant lactose fermentative opportunistic pathogenic Enterobacteriaceae bacteria and blaTEM-2 gene in cephalosporin wastewater and its discharge receiving river.
30245270	1	17	theme	CPWW	449:452	arg1	plant					465:469	cephalosporin wastewater (CPWW) treatment plant	423:469	cephalosporin wastewater (CPWW) treatment plant	423:469	This study investigated the concentration of cephalosporin, the resistant levels of lactose fermentative opportunistic pathogenic Enterobacteriaceae bacteria (LFOPEB) against seven antibiotics and one cephalosporin-resistant gene in cephalosporin wastewater (CPWW) treatment plant and its discharge receiving river.
30245270	2	18	theme	non-β-lactam	762:773	arg1	antibiotics					775:785	non-β-lactam antibiotics	762:785	non-β-lactam antibiotics	762:785	Although large numbers of bacteria have been removed during the CPWW treatment process, the antibiotic resistant rates of the isolates to β-lactam antibiotics significantly increased (p = 0.032) after treatment, while the percentage of resistant LFOPEB to non-β-lactam antibiotics did not change dramatically.
30245270	4	19	theme	antibiotic	1375:1384	arg1	water					1399:1403	antibiotic contaminated water	1375:1403	antibiotic contaminated water	1375:1403	The antibiotic resistant phenotypes of isolates in the influent of CPWW treatment plant (CPWWin), CPWWeff and RWdown appeared to be seriously affected by the cephalosporin residues, which suggested that main antibiotic resistance phenotypes in antibiotic contaminated water were closely associated with its antibiotic composition.
30245270	5	20	theme	ARG	1570:1572	arg1	proliferation					1553:1565	proliferation	1553:1565	proliferation of ARG	1553:1572	Therefore, CPWW treatment process has been proved to result in selective growth of ARB and proliferation of ARG.
30245270	5	20	theme	ARG	1570:1572	arg1	growth					1535:1540	selective growth	1525:1540	selective growth of ARB	1525:1547	Therefore, CPWW treatment process has been proved to result in selective growth of ARB and proliferation of ARG.
30245270	3	21	from	increase	911:918	arg1	river					1051:1055	the downstream receiving river	1026:1055	the downstream receiving river (RWdown) in comparison with those in the upstream receiving river (RWup)	1026:1128	Furthermore, the discharge of the effluent of CPWW treatment plant (CPWWeff) led to an obvious increase in the percentages of β-lactam antibiotic-resistant LFOPEB and relative abundance of the blaTEM-2 gene in the downstream receiving river (RWdown) in comparison with those in the upstream receiving river (RWup).
30245270	3	21	from	increase	911:918	arg1	RWdown					1058:1063	RWdown	1058:1063	RWdown	1058:1063	Furthermore, the discharge of the effluent of CPWW treatment plant (CPWWeff) led to an obvious increase in the percentages of β-lactam antibiotic-resistant LFOPEB and relative abundance of the blaTEM-2 gene in the downstream receiving river (RWdown) in comparison with those in the upstream receiving river (RWup).
30245270	3	21	from	increase	911:918	arg1	percentages					927:937	the percentages	923:937	the percentages of β-lactam antibiotic-resistant LFOPEB	923:977	Furthermore, the discharge of the effluent of CPWW treatment plant (CPWWeff) led to an obvious increase in the percentages of β-lactam antibiotic-resistant LFOPEB and relative abundance of the blaTEM-2 gene in the downstream receiving river (RWdown) in comparison with those in the upstream receiving river (RWup).
30245270	3	22	theme	relative	983:990	arg1	abundance					992:1000	relative abundance	983:1000	relative abundance of the blaTEM-2 gene	983:1021	Furthermore, the discharge of the effluent of CPWW treatment plant (CPWWeff) led to an obvious increase in the percentages of β-lactam antibiotic-resistant LFOPEB and relative abundance of the blaTEM-2 gene in the downstream receiving river (RWdown) in comparison with those in the upstream receiving river (RWup).
30245270	4	23	theme	main	1334:1337	arg1	phenotypes					1361:1370	main antibiotic resistance phenotypes	1334:1370	main antibiotic resistance phenotypes in antibiotic contaminated water	1334:1403	The antibiotic resistant phenotypes of isolates in the influent of CPWW treatment plant (CPWWin), CPWWeff and RWdown appeared to be seriously affected by the cephalosporin residues, which suggested that main antibiotic resistance phenotypes in antibiotic contaminated water were closely associated with its antibiotic composition.
30245270	4	24	from	phenotypes	1361:1370	arg1	water					1399:1403	antibiotic contaminated water	1375:1403	antibiotic contaminated water	1375:1403	The antibiotic resistant phenotypes of isolates in the influent of CPWW treatment plant (CPWWin), CPWWeff and RWdown appeared to be seriously affected by the cephalosporin residues, which suggested that main antibiotic resistance phenotypes in antibiotic contaminated water were closely associated with its antibiotic composition.
30245270	2	25	theme	LFOPEB	752:757	arg1	percentage					728:737	the percentage	724:737	the percentage of resistant LFOPEB to non-β-lactam antibiotics	724:785	Although large numbers of bacteria have been removed during the CPWW treatment process, the antibiotic resistant rates of the isolates to β-lactam antibiotics significantly increased (p = 0.032) after treatment, while the percentage of resistant LFOPEB to non-β-lactam antibiotics did not change dramatically.
30245270	0	26	theme	opportunistic	56:68	arg1	bacteria					100:107	opportunistic pathogenic Enterobacteriaceae bacteria	56:107	opportunistic pathogenic Enterobacteriaceae bacteria	56:107	Evaluation of antibiotic resistant lactose fermentative opportunistic pathogenic Enterobacteriaceae bacteria and blaTEM-2 gene in cephalosporin wastewater and its discharge receiving river.
30245270	4	27	from	influent	1186:1193	arg1	phenotypes					1156:1165	The antibiotic resistant phenotypes	1131:1165	The antibiotic resistant phenotypes of isolates in the influent of CPWW treatment plant (CPWWin), CPWWeff and RWdown	1131:1246	The antibiotic resistant phenotypes of isolates in the influent of CPWW treatment plant (CPWWin), CPWWeff and RWdown appeared to be seriously affected by the cephalosporin residues, which suggested that main antibiotic resistance phenotypes in antibiotic contaminated water were closely associated with its antibiotic composition.
30245270	0	28	theme	resistant	25:33	arg1	lactose					35:41	antibiotic resistant lactose	14:41	antibiotic resistant lactose	14:41	Evaluation of antibiotic resistant lactose fermentative opportunistic pathogenic Enterobacteriaceae bacteria and blaTEM-2 gene in cephalosporin wastewater and its discharge receiving river.
30245270	3	29	theme	plant	877:881	arg1	CPWWeff					884:890	CPWW treatment plant (CPWWeff)	862:891	CPWW treatment plant (CPWWeff)	862:891	Furthermore, the discharge of the effluent of CPWW treatment plant (CPWWeff) led to an obvious increase in the percentages of β-lactam antibiotic-resistant LFOPEB and relative abundance of the blaTEM-2 gene in the downstream receiving river (RWdown) in comparison with those in the upstream receiving river (RWup).
30245270	2	30	theme	resistant	742:750	arg1	LFOPEB					752:757	resistant LFOPEB	742:757	resistant LFOPEB to non-β-lactam antibiotics	742:785	Although large numbers of bacteria have been removed during the CPWW treatment process, the antibiotic resistant rates of the isolates to β-lactam antibiotics significantly increased (p = 0.032) after treatment, while the percentage of resistant LFOPEB to non-β-lactam antibiotics did not change dramatically.
30245270	1	31	theme	cephalosporin	235:247	arg1	concentration					218:230	the concentration	214:230	the concentration of cephalosporin	214:247	This study investigated the concentration of cephalosporin, the resistant levels of lactose fermentative opportunistic pathogenic Enterobacteriaceae bacteria (LFOPEB) against seven antibiotics and one cephalosporin-resistant gene in cephalosporin wastewater (CPWW) treatment plant and its discharge receiving river.
30245270	0	32	theme	antibiotic	14:23	arg1	lactose					35:41	antibiotic resistant lactose	14:41	antibiotic resistant lactose	14:41	Evaluation of antibiotic resistant lactose fermentative opportunistic pathogenic Enterobacteriaceae bacteria and blaTEM-2 gene in cephalosporin wastewater and its discharge receiving river.
30245270	6	33	theme	ARB	1639:1641	arg1	CPWWeff					1584:1590	CPWWeff	1584:1590	CPWWeff	1584:1590	Besides, CPWWeff was also proved to be an important supplier of ARB and ARG to the receiving river.
30245270	6	33	theme	ARB	1639:1641	arg1	supplier					1627:1634	an important supplier	1614:1634	an important supplier of ARB and ARG to the receiving river	1614:1672	Besides, CPWWeff was also proved to be an important supplier of ARB and ARG to the receiving river.
30245270	1	34	theme	treatment	455:463	arg1	plant					465:469	cephalosporin wastewater (CPWW) treatment plant	423:469	cephalosporin wastewater (CPWW) treatment plant	423:469	This study investigated the concentration of cephalosporin, the resistant levels of lactose fermentative opportunistic pathogenic Enterobacteriaceae bacteria (LFOPEB) against seven antibiotics and one cephalosporin-resistant gene in cephalosporin wastewater (CPWW) treatment plant and its discharge receiving river.
30245270	5	35	theme	treatment	1478:1486	arg1	process					1488:1494	CPWW treatment process	1473:1494	CPWW treatment process	1473:1494	Therefore, CPWW treatment process has been proved to result in selective growth of ARB and proliferation of ARG.
30245270	3	36	theme	gene	1018:1021	arg1	abundance					992:1000	relative abundance	983:1000	relative abundance of the blaTEM-2 gene	983:1021	Furthermore, the discharge of the effluent of CPWW treatment plant (CPWWeff) led to an obvious increase in the percentages of β-lactam antibiotic-resistant LFOPEB and relative abundance of the blaTEM-2 gene in the downstream receiving river (RWdown) in comparison with those in the upstream receiving river (RWup).
30245270	3	36	theme	gene	1018:1021	arg1	increase					911:918	an obvious increase	900:918	an obvious increase in the percentages of β-lactam antibiotic-resistant LFOPEB	900:977	Furthermore, the discharge of the effluent of CPWW treatment plant (CPWWeff) led to an obvious increase in the percentages of β-lactam antibiotic-resistant LFOPEB and relative abundance of the blaTEM-2 gene in the downstream receiving river (RWdown) in comparison with those in the upstream receiving river (RWup).
30245270	2	37	dep	increased	679:687	arg1	p = 0.032					690:698	p = 0.032	690:698	p = 0.032	690:698	Although large numbers of bacteria have been removed during the CPWW treatment process, the antibiotic resistant rates of the isolates to β-lactam antibiotics significantly increased (p = 0.032) after treatment, while the percentage of resistant LFOPEB to non-β-lactam antibiotics did not change dramatically.
30245270	6	38	theme	receiving	1658:1666	arg1	river					1668:1672	the receiving river	1654:1672	the receiving river	1654:1672	Besides, CPWWeff was also proved to be an important supplier of ARB and ARG to the receiving river.
30245270	3	39	theme	blaTEM-2	1009:1016	arg1	gene					1018:1021	the blaTEM-2 gene	1005:1021	the blaTEM-2 gene	1005:1021	Furthermore, the discharge of the effluent of CPWW treatment plant (CPWWeff) led to an obvious increase in the percentages of β-lactam antibiotic-resistant LFOPEB and relative abundance of the blaTEM-2 gene in the downstream receiving river (RWdown) in comparison with those in the upstream receiving river (RWup).
30245270	4	40	from	isolates	1170:1177	arg1	influent					1186:1193	the influent	1182:1193	the influent of CPWW treatment plant (CPWWin), CPWWeff and RWdown	1182:1246	The antibiotic resistant phenotypes of isolates in the influent of CPWW treatment plant (CPWWin), CPWWeff and RWdown appeared to be seriously affected by the cephalosporin residues, which suggested that main antibiotic resistance phenotypes in antibiotic contaminated water were closely associated with its antibiotic composition.
30245270	0	41	theme	lactose	35:41	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of antibiotic resistant lactose	0:41	Evaluation of antibiotic resistant lactose fermentative opportunistic pathogenic Enterobacteriaceae bacteria and blaTEM-2 gene in cephalosporin wastewater and its discharge receiving river.
30245270	3	42	theme	CPWW	862:865	arg1	CPWWeff					884:890	CPWW treatment plant (CPWWeff)	862:891	CPWW treatment plant (CPWWeff)	862:891	Furthermore, the discharge of the effluent of CPWW treatment plant (CPWWeff) led to an obvious increase in the percentages of β-lactam antibiotic-resistant LFOPEB and relative abundance of the blaTEM-2 gene in the downstream receiving river (RWdown) in comparison with those in the upstream receiving river (RWup).
30245270	2	43	theme	resistant	609:617	arg1	rates					619:623	the antibiotic resistant rates	594:623	the antibiotic resistant rates of the isolates to β-lactam antibiotics	594:663	Although large numbers of bacteria have been removed during the CPWW treatment process, the antibiotic resistant rates of the isolates to β-lactam antibiotics significantly increased (p = 0.032) after treatment, while the percentage of resistant LFOPEB to non-β-lactam antibiotics did not change dramatically.
30245270	4	44	theme	resistance	1350:1359	arg1	phenotypes					1361:1370	main antibiotic resistance phenotypes	1334:1370	main antibiotic resistance phenotypes in antibiotic contaminated water	1334:1403	The antibiotic resistant phenotypes of isolates in the influent of CPWW treatment plant (CPWWin), CPWWeff and RWdown appeared to be seriously affected by the cephalosporin residues, which suggested that main antibiotic resistance phenotypes in antibiotic contaminated water were closely associated with its antibiotic composition.
30245270	2	45	theme	large	515:519	arg1	numbers					521:527	large numbers	515:527	large numbers of bacteria	515:539	Although large numbers of bacteria have been removed during the CPWW treatment process, the antibiotic resistant rates of the isolates to β-lactam antibiotics significantly increased (p = 0.032) after treatment, while the percentage of resistant LFOPEB to non-β-lactam antibiotics did not change dramatically.
30245270	3	46	theme	receiving	1041:1049	arg1	RWdown					1058:1063	RWdown	1058:1063	RWdown	1058:1063	Furthermore, the discharge of the effluent of CPWW treatment plant (CPWWeff) led to an obvious increase in the percentages of β-lactam antibiotic-resistant LFOPEB and relative abundance of the blaTEM-2 gene in the downstream receiving river (RWdown) in comparison with those in the upstream receiving river (RWup).
30245270	3	46	theme	receiving	1041:1049	arg1	river					1051:1055	the downstream receiving river	1026:1055	the downstream receiving river (RWdown) in comparison with those in the upstream receiving river (RWup)	1026:1128	Furthermore, the discharge of the effluent of CPWW treatment plant (CPWWeff) led to an obvious increase in the percentages of β-lactam antibiotic-resistant LFOPEB and relative abundance of the blaTEM-2 gene in the downstream receiving river (RWdown) in comparison with those in the upstream receiving river (RWup).
30245270	1	47	theme	resistant	254:262	arg1	levels					264:269	the resistant levels	250:269	the resistant levels of lactose	250:280	This study investigated the concentration of cephalosporin, the resistant levels of lactose fermentative opportunistic pathogenic Enterobacteriaceae bacteria (LFOPEB) against seven antibiotics and one cephalosporin-resistant gene in cephalosporin wastewater (CPWW) treatment plant and its discharge receiving river.
30245270	0	48	theme	pathogenic	70:79	arg1	bacteria					100:107	opportunistic pathogenic Enterobacteriaceae bacteria	56:107	opportunistic pathogenic Enterobacteriaceae bacteria	56:107	Evaluation of antibiotic resistant lactose fermentative opportunistic pathogenic Enterobacteriaceae bacteria and blaTEM-2 gene in cephalosporin wastewater and its discharge receiving river.
30245270	3	49	theme	treatment	867:875	arg1	CPWWeff					884:890	CPWW treatment plant (CPWWeff)	862:891	CPWW treatment plant (CPWWeff)	862:891	Furthermore, the discharge of the effluent of CPWW treatment plant (CPWWeff) led to an obvious increase in the percentages of β-lactam antibiotic-resistant LFOPEB and relative abundance of the blaTEM-2 gene in the downstream receiving river (RWdown) in comparison with those in the upstream receiving river (RWup).
30245270	2	50	theme	CPWW	570:573	arg1	process					585:591	the CPWW treatment process	566:591	the CPWW treatment process	566:591	Although large numbers of bacteria have been removed during the CPWW treatment process, the antibiotic resistant rates of the isolates to β-lactam antibiotics significantly increased (p = 0.032) after treatment, while the percentage of resistant LFOPEB to non-β-lactam antibiotics did not change dramatically.
30245270	4	51	theme	contaminated	1386:1397	arg1	water					1399:1403	antibiotic contaminated water	1375:1403	antibiotic contaminated water	1375:1403	The antibiotic resistant phenotypes of isolates in the influent of CPWW treatment plant (CPWWin), CPWWeff and RWdown appeared to be seriously affected by the cephalosporin residues, which suggested that main antibiotic resistance phenotypes in antibiotic contaminated water were closely associated with its antibiotic composition.
30245270	4	52	from	phenotypes	1156:1165	arg1	influent					1186:1193	the influent	1182:1193	the influent of CPWW treatment plant (CPWWin), CPWWeff and RWdown	1182:1246	The antibiotic resistant phenotypes of isolates in the influent of CPWW treatment plant (CPWWin), CPWWeff and RWdown appeared to be seriously affected by the cephalosporin residues, which suggested that main antibiotic resistance phenotypes in antibiotic contaminated water were closely associated with its antibiotic composition.
30245270	1	53	from	gene	415:418	arg1	plant					465:469	cephalosporin wastewater (CPWW) treatment plant	423:469	cephalosporin wastewater (CPWW) treatment plant	423:469	This study investigated the concentration of cephalosporin, the resistant levels of lactose fermentative opportunistic pathogenic Enterobacteriaceae bacteria (LFOPEB) against seven antibiotics and one cephalosporin-resistant gene in cephalosporin wastewater (CPWW) treatment plant and its discharge receiving river.
30245270	1	53	from	gene	415:418	arg1	discharge					479:487	its discharge	475:487	its discharge receiving river	475:503	This study investigated the concentration of cephalosporin, the resistant levels of lactose fermentative opportunistic pathogenic Enterobacteriaceae bacteria (LFOPEB) against seven antibiotics and one cephalosporin-resistant gene in cephalosporin wastewater (CPWW) treatment plant and its discharge receiving river.
30245270	3	54	theme	effluent	850:857	arg1	discharge					833:841	the discharge	829:841	the discharge of the effluent of CPWW treatment plant (CPWWeff)	829:891	Furthermore, the discharge of the effluent of CPWW treatment plant (CPWWeff) led to an obvious increase in the percentages of β-lactam antibiotic-resistant LFOPEB and relative abundance of the blaTEM-2 gene in the downstream receiving river (RWdown) in comparison with those in the upstream receiving river (RWup).
30245270	2	55	theme	β-lactam	644:651	arg1	antibiotics					653:663	β-lactam antibiotics	644:663	β-lactam antibiotics	644:663	Although large numbers of bacteria have been removed during the CPWW treatment process, the antibiotic resistant rates of the isolates to β-lactam antibiotics significantly increased (p = 0.032) after treatment, while the percentage of resistant LFOPEB to non-β-lactam antibiotics did not change dramatically.
30245270	2	56	theme	bacteria	532:539	arg1	numbers					521:527	large numbers	515:527	large numbers of bacteria	515:539	Although large numbers of bacteria have been removed during the CPWW treatment process, the antibiotic resistant rates of the isolates to β-lactam antibiotics significantly increased (p = 0.032) after treatment, while the percentage of resistant LFOPEB to non-β-lactam antibiotics did not change dramatically.
30245270	4	57	theme	RWdown	1241:1246	arg1	influent					1186:1193	the influent	1182:1193	the influent of CPWW treatment plant (CPWWin), CPWWeff and RWdown	1182:1246	The antibiotic resistant phenotypes of isolates in the influent of CPWW treatment plant (CPWWin), CPWWeff and RWdown appeared to be seriously affected by the cephalosporin residues, which suggested that main antibiotic resistance phenotypes in antibiotic contaminated water were closely associated with its antibiotic composition.
30245270	4	58	theme	isolates	1170:1177	arg1	phenotypes					1156:1165	The antibiotic resistant phenotypes	1131:1165	The antibiotic resistant phenotypes of isolates in the influent of CPWW treatment plant (CPWWin), CPWWeff and RWdown	1131:1246	The antibiotic resistant phenotypes of isolates in the influent of CPWW treatment plant (CPWWin), CPWWeff and RWdown appeared to be seriously affected by the cephalosporin residues, which suggested that main antibiotic resistance phenotypes in antibiotic contaminated water were closely associated with its antibiotic composition.
30245270	4	59	theme	antibiotic	1135:1144	arg1	phenotypes					1156:1165	The antibiotic resistant phenotypes	1131:1165	The antibiotic resistant phenotypes of isolates in the influent of CPWW treatment plant (CPWWin), CPWWeff and RWdown	1131:1246	The antibiotic resistant phenotypes of isolates in the influent of CPWW treatment plant (CPWWin), CPWWeff and RWdown appeared to be seriously affected by the cephalosporin residues, which suggested that main antibiotic resistance phenotypes in antibiotic contaminated water were closely associated with its antibiotic composition.
30245270	3	60	theme	upstream	1098:1105	arg1	RWup					1124:1127	RWup	1124:1127	RWup	1124:1127	Furthermore, the discharge of the effluent of CPWW treatment plant (CPWWeff) led to an obvious increase in the percentages of β-lactam antibiotic-resistant LFOPEB and relative abundance of the blaTEM-2 gene in the downstream receiving river (RWdown) in comparison with those in the upstream receiving river (RWup).
30245270	3	60	theme	upstream	1098:1105	arg1	river					1117:1121	the upstream receiving river	1094:1121	the upstream receiving river (RWup)	1094:1128	Furthermore, the discharge of the effluent of CPWW treatment plant (CPWWeff) led to an obvious increase in the percentages of β-lactam antibiotic-resistant LFOPEB and relative abundance of the blaTEM-2 gene in the downstream receiving river (RWdown) in comparison with those in the upstream receiving river (RWup).
30245270	3	61	theme	receiving	1107:1115	arg1	RWup					1124:1127	RWup	1124:1127	RWup	1124:1127	Furthermore, the discharge of the effluent of CPWW treatment plant (CPWWeff) led to an obvious increase in the percentages of β-lactam antibiotic-resistant LFOPEB and relative abundance of the blaTEM-2 gene in the downstream receiving river (RWdown) in comparison with those in the upstream receiving river (RWup).
30245270	3	61	theme	receiving	1107:1115	arg1	river					1117:1121	the upstream receiving river	1094:1121	the upstream receiving river (RWup)	1094:1128	Furthermore, the discharge of the effluent of CPWW treatment plant (CPWWeff) led to an obvious increase in the percentages of β-lactam antibiotic-resistant LFOPEB and relative abundance of the blaTEM-2 gene in the downstream receiving river (RWdown) in comparison with those in the upstream receiving river (RWup).
30245270	5	62	theme	CPWW	1473:1476	arg1	process					1488:1494	CPWW treatment process	1473:1494	CPWW treatment process	1473:1494	Therefore, CPWW treatment process has been proved to result in selective growth of ARB and proliferation of ARG.
30245270	6	63	theme	ARG	1647:1649	arg1	CPWWeff					1584:1590	CPWWeff	1584:1590	CPWWeff	1584:1590	Besides, CPWWeff was also proved to be an important supplier of ARB and ARG to the receiving river.
30245270	6	63	theme	ARG	1647:1649	arg1	supplier					1627:1634	an important supplier	1614:1634	an important supplier of ARB and ARG to the receiving river	1614:1672	Besides, CPWWeff was also proved to be an important supplier of ARB and ARG to the receiving river.
30245270	3	64	theme	CPWWeff	884:890	arg1	effluent					850:857	the effluent	846:857	the effluent of CPWW treatment plant (CPWWeff)	846:891	Furthermore, the discharge of the effluent of CPWW treatment plant (CPWWeff) led to an obvious increase in the percentages of β-lactam antibiotic-resistant LFOPEB and relative abundance of the blaTEM-2 gene in the downstream receiving river (RWdown) in comparison with those in the upstream receiving river (RWup).
30245270	2	65	theme	isolates	632:639	arg1	rates					619:623	the antibiotic resistant rates	594:623	the antibiotic resistant rates of the isolates to β-lactam antibiotics	594:663	Although large numbers of bacteria have been removed during the CPWW treatment process, the antibiotic resistant rates of the isolates to β-lactam antibiotics significantly increased (p = 0.032) after treatment, while the percentage of resistant LFOPEB to non-β-lactam antibiotics did not change dramatically.
30245270	4	66	theme	CPWWeff	1229:1235	arg1	influent					1186:1193	the influent	1182:1193	the influent of CPWW treatment plant (CPWWin), CPWWeff and RWdown	1182:1246	The antibiotic resistant phenotypes of isolates in the influent of CPWW treatment plant (CPWWin), CPWWeff and RWdown appeared to be seriously affected by the cephalosporin residues, which suggested that main antibiotic resistance phenotypes in antibiotic contaminated water were closely associated with its antibiotic composition.
30245270	3	67	with	comparison	1069:1078	arg1	those					1085:1089	those	1085:1089	those	1085:1089	Furthermore, the discharge of the effluent of CPWW treatment plant (CPWWeff) led to an obvious increase in the percentages of β-lactam antibiotic-resistant LFOPEB and relative abundance of the blaTEM-2 gene in the downstream receiving river (RWdown) in comparison with those in the upstream receiving river (RWup).
30245270	2	68	theme	treatment	575:583	arg1	process					585:591	the CPWW treatment process	566:591	the CPWW treatment process	566:591	Although large numbers of bacteria have been removed during the CPWW treatment process, the antibiotic resistant rates of the isolates to β-lactam antibiotics significantly increased (p = 0.032) after treatment, while the percentage of resistant LFOPEB to non-β-lactam antibiotics did not change dramatically.
30245270	3	69	from	river	1051:1055	arg1	comparison					1069:1078	comparison	1069:1078	comparison with those in the upstream receiving river (RWup)	1069:1128	Furthermore, the discharge of the effluent of CPWW treatment plant (CPWWeff) led to an obvious increase in the percentages of β-lactam antibiotic-resistant LFOPEB and relative abundance of the blaTEM-2 gene in the downstream receiving river (RWdown) in comparison with those in the upstream receiving river (RWup).
30245270	3	70	from	abundance	992:1000	arg1	river					1051:1055	the downstream receiving river	1026:1055	the downstream receiving river (RWdown) in comparison with those in the upstream receiving river (RWup)	1026:1128	Furthermore, the discharge of the effluent of CPWW treatment plant (CPWWeff) led to an obvious increase in the percentages of β-lactam antibiotic-resistant LFOPEB and relative abundance of the blaTEM-2 gene in the downstream receiving river (RWdown) in comparison with those in the upstream receiving river (RWup).
30245270	3	70	from	abundance	992:1000	arg1	RWdown					1058:1063	RWdown	1058:1063	RWdown	1058:1063	Furthermore, the discharge of the effluent of CPWW treatment plant (CPWWeff) led to an obvious increase in the percentages of β-lactam antibiotic-resistant LFOPEB and relative abundance of the blaTEM-2 gene in the downstream receiving river (RWdown) in comparison with those in the upstream receiving river (RWup).
30245270	3	70	from	abundance	992:1000	arg1	percentages					927:937	the percentages	923:937	the percentages of β-lactam antibiotic-resistant LFOPEB	923:977	Furthermore, the discharge of the effluent of CPWW treatment plant (CPWWeff) led to an obvious increase in the percentages of β-lactam antibiotic-resistant LFOPEB and relative abundance of the blaTEM-2 gene in the downstream receiving river (RWdown) in comparison with those in the upstream receiving river (RWup).
30245270	6	71	theme	important	1617:1625	arg1	CPWWeff					1584:1590	CPWWeff	1584:1590	CPWWeff	1584:1590	Besides, CPWWeff was also proved to be an important supplier of ARB and ARG to the receiving river.
30245270	6	71	theme	important	1617:1625	arg1	supplier					1627:1634	an important supplier	1614:1634	an important supplier of ARB and ARG to the receiving river	1614:1672	Besides, CPWWeff was also proved to be an important supplier of ARB and ARG to the receiving river.
30245270	4	72	theme	resistant	1146:1154	arg1	phenotypes					1156:1165	The antibiotic resistant phenotypes	1131:1165	The antibiotic resistant phenotypes of isolates in the influent of CPWW treatment plant (CPWWin), CPWWeff and RWdown	1131:1246	The antibiotic resistant phenotypes of isolates in the influent of CPWW treatment plant (CPWWin), CPWWeff and RWdown appeared to be seriously affected by the cephalosporin residues, which suggested that main antibiotic resistance phenotypes in antibiotic contaminated water were closely associated with its antibiotic composition.
30245270	1	73	theme	lactose	274:280	arg1	levels					264:269	the resistant levels	250:269	the resistant levels of lactose	250:280	This study investigated the concentration of cephalosporin, the resistant levels of lactose fermentative opportunistic pathogenic Enterobacteriaceae bacteria (LFOPEB) against seven antibiotics and one cephalosporin-resistant gene in cephalosporin wastewater (CPWW) treatment plant and its discharge receiving river.
30245270	0	74	theme	Enterobacteriaceae	81:98	arg1	bacteria					100:107	opportunistic pathogenic Enterobacteriaceae bacteria	56:107	opportunistic pathogenic Enterobacteriaceae bacteria	56:107	Evaluation of antibiotic resistant lactose fermentative opportunistic pathogenic Enterobacteriaceae bacteria and blaTEM-2 gene in cephalosporin wastewater and its discharge receiving river.
30245270	1	75	theme	cephalosporin-resistant	391:413	arg1	gene					415:418	one cephalosporin-resistant gene	387:418	one cephalosporin-resistant gene	387:418	This study investigated the concentration of cephalosporin, the resistant levels of lactose fermentative opportunistic pathogenic Enterobacteriaceae bacteria (LFOPEB) against seven antibiotics and one cephalosporin-resistant gene in cephalosporin wastewater (CPWW) treatment plant and its discharge receiving river.
30245270	5	76	theme	ARB	1545:1547	arg1	proliferation					1553:1565	proliferation	1553:1565	proliferation of ARG	1553:1572	Therefore, CPWW treatment process has been proved to result in selective growth of ARB and proliferation of ARG.
30245270	5	76	theme	ARB	1545:1547	arg1	growth					1535:1540	selective growth	1525:1540	selective growth of ARB	1525:1547	Therefore, CPWW treatment process has been proved to result in selective growth of ARB and proliferation of ARG.
30245270	3	77	theme	antibiotic-resistant	951:970	arg1	LFOPEB					972:977	β-lactam antibiotic-resistant LFOPEB	942:977	β-lactam antibiotic-resistant LFOPEB	942:977	Furthermore, the discharge of the effluent of CPWW treatment plant (CPWWeff) led to an obvious increase in the percentages of β-lactam antibiotic-resistant LFOPEB and relative abundance of the blaTEM-2 gene in the downstream receiving river (RWdown) in comparison with those in the upstream receiving river (RWup).
30654210	7	0	theme	reduced	1484:1490	arg1	effects					1497:1503	reduced side effects	1484:1503	dose reduction as well as reduced side effects	1458:1503	STATEMENT OF SIGNIFICANCE: The localized delivery of bioactive molecules has attracted significant attention due to the potential for dose reduction as well as reduced side effects compared to systemic delivery.
30654210	4	1	theme	aureus	892:897	arg1	growth					909:914	Staphylococcus aureus bacterial growth	877:914	Staphylococcus aureus bacterial growth	877:914	The dual release of both molecules showed a marked increase in the number of blood vessels on embryonic day 14 in chicken embryos grown ex ovo, while, at the same time providing an antibiotic local concentration capable of inhibiting Staphylococcus aureus bacterial growth.
30654210	3	2	theme	mesoporous	535:544	arg1	nanoparticles					553:565	mesoporous silica nanoparticles	535:565	mesoporous silica nanoparticles	535:565	An angiogenic protein, Vascular Endothelial Growth Factor, and an antibiotic, cephalexin, loaded in mesoporous silica nanoparticles, were included to design multifunctional scaffolds for bone reconstruction.
30654210	4	3	theme	capable	855:861	arg1	concentration					841:853	an antibiotic local concentration	821:853	an antibiotic local concentration capable of inhibiting Staphylococcus aureus bacterial growth	821:914	The dual release of both molecules showed a marked increase in the number of blood vessels on embryonic day 14 in chicken embryos grown ex ovo, while, at the same time providing an antibiotic local concentration capable of inhibiting Staphylococcus aureus bacterial growth.
30654210	2	4	theme	fabrication	412:422	arg1	procedure					424:432	the fabrication procedure	408:432	the fabrication procedure	408:432	This shaping technology allows the introduction of thermally-labile molecules within the scaffolds during the fabrication procedure.
30654210	1	5	theme	structural	184:193	arg1	components					195:204	two structural components	180:204	two structural components that act as a temporary mineralized extracellular matrix	180:261	3D porous scaffolds based on agarose and nanocrystalline apatite, two structural components that act as a temporary mineralized extracellular matrix, were prepared by the GELPOR3D method.
30654210	1	5	theme	structural	184:193	arg1	matrix					256:261	a temporary mineralized extracellular matrix	218:261	a temporary mineralized extracellular matrix	218:261	3D porous scaffolds based on agarose and nanocrystalline apatite, two structural components that act as a temporary mineralized extracellular matrix, were prepared by the GELPOR3D method.
30654210	1	5	theme	structural	184:193	arg1	agarose					143:149	agarose	143:149	agarose	143:149	3D porous scaffolds based on agarose and nanocrystalline apatite, two structural components that act as a temporary mineralized extracellular matrix, were prepared by the GELPOR3D method.
30654210	9	6	theme	drug	1699:1702	arg1	loading					1674:1680	the controlled loading	1659:1680	the controlled loading of an antibiotic drug and an angiogenic protein into the scaffold	1659:1746	The method also enables the controlled loading of an antibiotic drug and an angiogenic protein into the scaffold.
30654210	7	7	theme	molecules	1387:1395	arg1	delivery					1365:1372	The localized delivery	1351:1372	The localized delivery of bioactive molecules	1351:1395	STATEMENT OF SIGNIFICANCE: The localized delivery of bioactive molecules has attracted significant attention due to the potential for dose reduction as well as reduced side effects compared to systemic delivery.
30654210	10	8	dep	matrix	1812:1817	arg1	suitable					1823:1830	suitable	1823:1830	suitable	1823:1830	These scaffolds, whose composition resembles the extracellular matrix are suitable for the adhesion of preosteoblast cells, exhibit a sustained cephalexin delivery adequate for inhibiting bacterial growth as well as release the proangiogenic molecule which induces blood vessel formation in chicken embryos grown ex ovo.
30654210	3	9	theme	multifunctional	592:606	arg1	scaffolds					608:616	multifunctional scaffolds	592:616	multifunctional scaffolds for bone reconstruction	592:640	An angiogenic protein, Vascular Endothelial Growth Factor, and an antibiotic, cephalexin, loaded in mesoporous silica nanoparticles, were included to design multifunctional scaffolds for bone reconstruction.
30654210	5	10	dep	tailored	999:1006	arg1	either					1058:1063	either	1058:1063	either	1058:1063	In this sense, different release patterns, monitored by UV-spectroscopy, could be tailored as a function of the cephalexin loading strategy, either releasing all the loaded cephalexin in the first 4 h or less than 50% after 24 h.
30654210	4	11	theme	marked	687:692	arg1	increase					694:701	a marked increase	685:701	a marked increase in the number of blood vessels	685:732	The dual release of both molecules showed a marked increase in the number of blood vessels on embryonic day 14 in chicken embryos grown ex ovo, while, at the same time providing an antibiotic local concentration capable of inhibiting Staphylococcus aureus bacterial growth.
30654210	10	12	theme	vessel	2020:2025	arg1	formation					2027:2035	blood vessel formation	2014:2035	blood vessel formation	2014:2035	These scaffolds, whose composition resembles the extracellular matrix are suitable for the adhesion of preosteoblast cells, exhibit a sustained cephalexin delivery adequate for inhibiting bacterial growth as well as release the proangiogenic molecule which induces blood vessel formation in chicken embryos grown ex ovo.
30654210	6	13	theme	preosteoblastic	1301:1315	arg1	cells					1317:1321	preosteoblastic cells	1301:1321	preosteoblastic cells	1301:1321	The scaffold surface was characterized by a high hydrophilicity, with contact angles between 50° and 63°, which enabled the adhesion and proliferation of preosteoblastic cells.
30654210	1	14	theme	temporary	220:228	arg1	matrix					256:261	a temporary mineralized extracellular matrix	218:261	a temporary mineralized extracellular matrix	218:261	3D porous scaffolds based on agarose and nanocrystalline apatite, two structural components that act as a temporary mineralized extracellular matrix, were prepared by the GELPOR3D method.
30654210	1	14	theme	temporary	220:228	arg1	components					195:204	two structural components	180:204	two structural components that act as a temporary mineralized extracellular matrix	180:261	3D porous scaffolds based on agarose and nanocrystalline apatite, two structural components that act as a temporary mineralized extracellular matrix, were prepared by the GELPOR3D method.
30654210	9	15	theme	protein	1722:1728	arg1	loading					1674:1680	the controlled loading	1659:1680	the controlled loading of an antibiotic drug and an angiogenic protein into the scaffold	1659:1746	The method also enables the controlled loading of an antibiotic drug and an angiogenic protein into the scaffold.
30654210	7	16	theme	localized	1355:1363	arg1	delivery					1365:1372	The localized delivery	1351:1372	The localized delivery of bioactive molecules	1351:1395	STATEMENT OF SIGNIFICANCE: The localized delivery of bioactive molecules has attracted significant attention due to the potential for dose reduction as well as reduced side effects compared to systemic delivery.
30654210	10	17	theme	extracellular	1798:1810	arg1	matrix					1812:1817	the extracellular matrix	1794:1817	the extracellular matrix are suitable for the adhesion of preosteoblast cells	1794:1870	These scaffolds, whose composition resembles the extracellular matrix are suitable for the adhesion of preosteoblast cells, exhibit a sustained cephalexin delivery adequate for inhibiting bacterial growth as well as release the proangiogenic molecule which induces blood vessel formation in chicken embryos grown ex ovo.
30654210	5	18	theme	different	932:940	arg1	function					1013:1020	a function	1011:1020	a function of the cephalexin loading strategy	1011:1055	In this sense, different release patterns, monitored by UV-spectroscopy, could be tailored as a function of the cephalexin loading strategy, either releasing all the loaded cephalexin in the first 4 h or less than 50% after 24 h.
30654210	5	18	theme	different	932:940	arg1	patterns					950:957	different release patterns	932:957	different release patterns	932:957	In this sense, different release patterns, monitored by UV-spectroscopy, could be tailored as a function of the cephalexin loading strategy, either releasing all the loaded cephalexin in the first 4 h or less than 50% after 24 h.
30654210	4	19	theme	blood	720:724	arg1	vessels					726:732	blood vessels	720:732	blood vessels	720:732	The dual release of both molecules showed a marked increase in the number of blood vessels on embryonic day 14 in chicken embryos grown ex ovo, while, at the same time providing an antibiotic local concentration capable of inhibiting Staphylococcus aureus bacterial growth.
30654210	1	20	theme	nanocrystalline	155:169	arg1	apatite					171:177	nanocrystalline apatite	155:177	nanocrystalline apatite	155:177	3D porous scaffolds based on agarose and nanocrystalline apatite, two structural components that act as a temporary mineralized extracellular matrix, were prepared by the GELPOR3D method.
30654210	9	21	theme	controlled	1663:1672	arg1	loading					1674:1680	the controlled loading	1659:1680	the controlled loading of an antibiotic drug and an angiogenic protein into the scaffold	1659:1746	The method also enables the controlled loading of an antibiotic drug and an angiogenic protein into the scaffold.
30654210	10	22	dep	release	1965:1971	arg1	molecule					1991:1998	the proangiogenic molecule	1973:1998	bacterial growth as well as release the proangiogenic molecule which induces blood vessel formation in chicken embryos grown ex ovo	1937:2067	These scaffolds, whose composition resembles the extracellular matrix are suitable for the adhesion of preosteoblast cells, exhibit a sustained cephalexin delivery adequate for inhibiting bacterial growth as well as release the proangiogenic molecule which induces blood vessel formation in chicken embryos grown ex ovo.
30654210	3	23	theme	Growth	479:484	arg1	Factor					486:491	Vascular Endothelial Growth Factor	458:491	Vascular Endothelial Growth Factor	458:491	An angiogenic protein, Vascular Endothelial Growth Factor, and an antibiotic, cephalexin, loaded in mesoporous silica nanoparticles, were included to design multifunctional scaffolds for bone reconstruction.
30654210	3	24	theme	Vascular	458:465	arg1	Factor					486:491	Vascular Endothelial Growth Factor	458:491	Vascular Endothelial Growth Factor	458:491	An angiogenic protein, Vascular Endothelial Growth Factor, and an antibiotic, cephalexin, loaded in mesoporous silica nanoparticles, were included to design multifunctional scaffolds for bone reconstruction.
30654210	1	25	theme	GELPOR3D	285:292	arg1	method					294:299	the GELPOR3D method	281:299	the GELPOR3D method	281:299	3D porous scaffolds based on agarose and nanocrystalline apatite, two structural components that act as a temporary mineralized extracellular matrix, were prepared by the GELPOR3D method.
30654210	1	26	theme	porous	117:122	arg1	scaffolds					124:132	3D porous scaffolds	114:132	3D porous scaffolds based on agarose and nanocrystalline apatite, two structural components that act as a temporary mineralized extracellular matrix,	114:262	3D porous scaffolds based on agarose and nanocrystalline apatite, two structural components that act as a temporary mineralized extracellular matrix, were prepared by the GELPOR3D method.
30654210	8	27	theme	multifunctional	1552:1566	arg1	scaffolds					1578:1586	multifunctional 3D porous scaffolds	1552:1586	multifunctional 3D porous scaffolds with a designed porosity	1552:1611	In this article multifunctional 3D porous scaffolds with a designed porosity have been fabricated.
30654210	0	28	theme	3D	41:42	arg1	scaffold					56:63	a nanoparticle-containing 3D porous bone scaffold	15:63	a nanoparticle-containing 3D porous bone scaffold	15:63	Fabrication of a nanoparticle-containing 3D porous bone scaffold with proangiogenic and antibacterial properties.
30654210	5	29	from	%	1133:1133	arg1	4 h					1114:1116	the first 4 h	1104:1116	the first 4 h	1104:1116	In this sense, different release patterns, monitored by UV-spectroscopy, could be tailored as a function of the cephalexin loading strategy, either releasing all the loaded cephalexin in the first 4 h or less than 50% after 24 h.
30654210	7	30	theme	systemic	1517:1524	arg1	delivery					1526:1533	systemic delivery	1517:1533	systemic delivery	1517:1533	STATEMENT OF SIGNIFICANCE: The localized delivery of bioactive molecules has attracted significant attention due to the potential for dose reduction as well as reduced side effects compared to systemic delivery.
30654210	5	31	theme	cephalexin	1029:1038	arg1	strategy					1048:1055	the cephalexin loading strategy	1025:1055	the cephalexin loading strategy	1025:1055	In this sense, different release patterns, monitored by UV-spectroscopy, could be tailored as a function of the cephalexin loading strategy, either releasing all the loaded cephalexin in the first 4 h or less than 50% after 24 h.
30654210	8	32	theme	porous	1571:1576	arg1	scaffolds					1578:1586	multifunctional 3D porous scaffolds	1552:1586	multifunctional 3D porous scaffolds with a designed porosity	1552:1611	In this article multifunctional 3D porous scaffolds with a designed porosity have been fabricated.
30654210	10	33	theme	preosteoblast	1852:1864	arg1	cells					1866:1870	preosteoblast cells	1852:1870	preosteoblast cells	1852:1870	These scaffolds, whose composition resembles the extracellular matrix are suitable for the adhesion of preosteoblast cells, exhibit a sustained cephalexin delivery adequate for inhibiting bacterial growth as well as release the proangiogenic molecule which induces blood vessel formation in chicken embryos grown ex ovo.
30654210	4	34	theme	local	835:839	arg1	concentration					841:853	an antibiotic local concentration	821:853	an antibiotic local concentration capable of inhibiting Staphylococcus aureus bacterial growth	821:914	The dual release of both molecules showed a marked increase in the number of blood vessels on embryonic day 14 in chicken embryos grown ex ovo, while, at the same time providing an antibiotic local concentration capable of inhibiting Staphylococcus aureus bacterial growth.
30654210	2	35	theme	molecules	370:378	arg1	introduction					337:348	the introduction	333:348	the introduction of thermally-labile molecules	333:378	This shaping technology allows the introduction of thermally-labile molecules within the scaffolds during the fabrication procedure.
30654210	0	36	theme	bone	51:54	arg1	scaffold					56:63	a nanoparticle-containing 3D porous bone scaffold	15:63	a nanoparticle-containing 3D porous bone scaffold	15:63	Fabrication of a nanoparticle-containing 3D porous bone scaffold with proangiogenic and antibacterial properties.
30654210	7	37	dep	STATEMENT	1324:1332	arg1	attracted					1401:1409	attracted	1401:1409	has attracted significant attention due to the potential for dose reduction as well as reduced side effects compared to systemic delivery	1397:1533	STATEMENT OF SIGNIFICANCE: The localized delivery of bioactive molecules has attracted significant attention due to the potential for dose reduction as well as reduced side effects compared to systemic delivery.
30654210	10	38	theme	cephalexin	1893:1902	arg1	delivery					1904:1911	a sustained cephalexin delivery	1881:1911	a sustained cephalexin delivery adequate for inhibiting bacterial growth as well as release the proangiogenic molecule which induces blood vessel formation in chicken embryos grown ex ovo	1881:2067	These scaffolds, whose composition resembles the extracellular matrix are suitable for the adhesion of preosteoblast cells, exhibit a sustained cephalexin delivery adequate for inhibiting bacterial growth as well as release the proangiogenic molecule which induces blood vessel formation in chicken embryos grown ex ovo.
30654210	0	39	with	Fabrication	0:10	arg1	properties					102:111	proangiogenic and antibacterial properties	70:111	proangiogenic and antibacterial properties	70:111	Fabrication of a nanoparticle-containing 3D porous bone scaffold with proangiogenic and antibacterial properties.
30654210	1	40	theme	mineralized	230:240	arg1	matrix					256:261	a temporary mineralized extracellular matrix	218:261	a temporary mineralized extracellular matrix	218:261	3D porous scaffolds based on agarose and nanocrystalline apatite, two structural components that act as a temporary mineralized extracellular matrix, were prepared by the GELPOR3D method.
30654210	1	40	theme	mineralized	230:240	arg1	components					195:204	two structural components	180:204	two structural components that act as a temporary mineralized extracellular matrix	180:261	3D porous scaffolds based on agarose and nanocrystalline apatite, two structural components that act as a temporary mineralized extracellular matrix, were prepared by the GELPOR3D method.
30654210	6	41	theme	scaffold	1151:1158	arg1	surface					1160:1166	The scaffold surface	1147:1166	The scaffold surface	1147:1166	The scaffold surface was characterized by a high hydrophilicity, with contact angles between 50° and 63°, which enabled the adhesion and proliferation of preosteoblastic cells.
30654210	7	42	theme	side	1492:1495	arg1	effects					1497:1503	reduced side effects	1484:1503	dose reduction as well as reduced side effects	1458:1503	STATEMENT OF SIGNIFICANCE: The localized delivery of bioactive molecules has attracted significant attention due to the potential for dose reduction as well as reduced side effects compared to systemic delivery.
30654210	8	43	theme	designed	1595:1602	arg1	porosity					1604:1611	a designed porosity	1593:1611	a designed porosity	1593:1611	In this article multifunctional 3D porous scaffolds with a designed porosity have been fabricated.
30654210	3	44	theme	silica	546:551	arg1	nanoparticles					553:565	mesoporous silica nanoparticles	535:565	mesoporous silica nanoparticles	535:565	An angiogenic protein, Vascular Endothelial Growth Factor, and an antibiotic, cephalexin, loaded in mesoporous silica nanoparticles, were included to design multifunctional scaffolds for bone reconstruction.
30654210	10	45	theme	adequate	1913:1920	arg1	delivery					1904:1911	a sustained cephalexin delivery	1881:1911	a sustained cephalexin delivery adequate for inhibiting bacterial growth as well as release the proangiogenic molecule which induces blood vessel formation in chicken embryos grown ex ovo	1881:2067	These scaffolds, whose composition resembles the extracellular matrix are suitable for the adhesion of preosteoblast cells, exhibit a sustained cephalexin delivery adequate for inhibiting bacterial growth as well as release the proangiogenic molecule which induces blood vessel formation in chicken embryos grown ex ovo.
30654210	7	46	theme	dose	1458:1461	arg1	reduction					1463:1471	dose reduction	1458:1471	dose reduction as well as reduced side effects	1458:1503	STATEMENT OF SIGNIFICANCE: The localized delivery of bioactive molecules has attracted significant attention due to the potential for dose reduction as well as reduced side effects compared to systemic delivery.
30654210	4	47	theme	bacterial	899:907	arg1	growth					909:914	Staphylococcus aureus bacterial growth	877:914	Staphylococcus aureus bacterial growth	877:914	The dual release of both molecules showed a marked increase in the number of blood vessels on embryonic day 14 in chicken embryos grown ex ovo, while, at the same time providing an antibiotic local concentration capable of inhibiting Staphylococcus aureus bacterial growth.
30654210	7	48	theme	due	1433:1435	arg1	attention					1423:1431	significant attention	1411:1431	significant attention due to the potential for dose reduction as well as reduced side effects	1411:1503	STATEMENT OF SIGNIFICANCE: The localized delivery of bioactive molecules has attracted significant attention due to the potential for dose reduction as well as reduced side effects compared to systemic delivery.
30654210	4	49	from	increase	694:701	arg1	number					710:715	the number	706:715	the number of blood vessels	706:732	The dual release of both molecules showed a marked increase in the number of blood vessels on embryonic day 14 in chicken embryos grown ex ovo, while, at the same time providing an antibiotic local concentration capable of inhibiting Staphylococcus aureus bacterial growth.
30654210	6	50	dep	adhesion	1271:1278	arg1	the					1267:1269	the	1267:1269	the	1267:1269	The scaffold surface was characterized by a high hydrophilicity, with contact angles between 50° and 63°, which enabled the adhesion and proliferation of preosteoblastic cells.
30654210	9	51	theme	antibiotic	1688:1697	arg1	drug					1699:1702	an antibiotic drug	1685:1702	an antibiotic drug	1685:1702	The method also enables the controlled loading of an antibiotic drug and an angiogenic protein into the scaffold.
30654210	7	52	theme	significant	1411:1421	arg1	attention					1423:1431	significant attention	1411:1431	significant attention due to the potential for dose reduction as well as reduced side effects	1411:1503	STATEMENT OF SIGNIFICANCE: The localized delivery of bioactive molecules has attracted significant attention due to the potential for dose reduction as well as reduced side effects compared to systemic delivery.
30654210	10	53	theme	proangiogenic	1977:1989	arg1	molecule					1991:1998	the proangiogenic molecule	1973:1998	bacterial growth as well as release the proangiogenic molecule which induces blood vessel formation in chicken embryos grown ex ovo	1937:2067	These scaffolds, whose composition resembles the extracellular matrix are suitable for the adhesion of preosteoblast cells, exhibit a sustained cephalexin delivery adequate for inhibiting bacterial growth as well as release the proangiogenic molecule which induces blood vessel formation in chicken embryos grown ex ovo.
30654210	5	54	theme	strategy	1048:1055	arg1	function					1013:1020	a function	1011:1020	a function of the cephalexin loading strategy	1011:1055	In this sense, different release patterns, monitored by UV-spectroscopy, could be tailored as a function of the cephalexin loading strategy, either releasing all the loaded cephalexin in the first 4 h or less than 50% after 24 h.
30654210	5	54	theme	strategy	1048:1055	arg1	patterns					950:957	different release patterns	932:957	different release patterns	932:957	In this sense, different release patterns, monitored by UV-spectroscopy, could be tailored as a function of the cephalexin loading strategy, either releasing all the loaded cephalexin in the first 4 h or less than 50% after 24 h.
30654210	4	55	theme	molecules	668:676	arg1	release					652:658	The dual release	643:658	The dual release of both molecules	643:676	The dual release of both molecules showed a marked increase in the number of blood vessels on embryonic day 14 in chicken embryos grown ex ovo, while, at the same time providing an antibiotic local concentration capable of inhibiting Staphylococcus aureus bacterial growth.
30654210	6	56	theme	cells	1317:1321	arg1	proliferation					1284:1296	proliferation	1284:1296	proliferation	1284:1296	The scaffold surface was characterized by a high hydrophilicity, with contact angles between 50° and 63°, which enabled the adhesion and proliferation of preosteoblastic cells.
30654210	6	56	theme	cells	1317:1321	arg1	adhesion					1271:1278	adhesion	1271:1278	adhesion	1271:1278	The scaffold surface was characterized by a high hydrophilicity, with contact angles between 50° and 63°, which enabled the adhesion and proliferation of preosteoblastic cells.
30654210	5	57	theme	loaded	1083:1088	arg1	cephalexin					1090:1099	all the loaded cephalexin	1075:1099	all the loaded cephalexin in the first 4 h	1075:1116	In this sense, different release patterns, monitored by UV-spectroscopy, could be tailored as a function of the cephalexin loading strategy, either releasing all the loaded cephalexin in the first 4 h or less than 50% after 24 h.
30654210	9	58	theme	angiogenic	1711:1720	arg1	protein					1722:1728	an angiogenic protein	1708:1728	an angiogenic protein	1708:1728	The method also enables the controlled loading of an antibiotic drug and an angiogenic protein into the scaffold.
30654210	6	59	theme	contact	1217:1223	arg1	angles					1225:1230	contact angles	1217:1230	contact angles between 50° and 63°, which enabled the adhesion and proliferation of preosteoblastic cells	1217:1321	The scaffold surface was characterized by a high hydrophilicity, with contact angles between 50° and 63°, which enabled the adhesion and proliferation of preosteoblastic cells.
30654210	10	60	theme	blood	2014:2018	arg1	formation					2027:2035	blood vessel formation	2014:2035	blood vessel formation	2014:2035	These scaffolds, whose composition resembles the extracellular matrix are suitable for the adhesion of preosteoblast cells, exhibit a sustained cephalexin delivery adequate for inhibiting bacterial growth as well as release the proangiogenic molecule which induces blood vessel formation in chicken embryos grown ex ovo.
30654210	3	61	theme	bone	622:625	arg1	reconstruction					627:640	bone reconstruction	622:640	bone reconstruction	622:640	An angiogenic protein, Vascular Endothelial Growth Factor, and an antibiotic, cephalexin, loaded in mesoporous silica nanoparticles, were included to design multifunctional scaffolds for bone reconstruction.
30654210	4	62	theme	embryonic	737:745	arg1	day					747:749	embryonic day 14	737:752	embryonic day 14 in chicken embryos grown ex ovo	737:784	The dual release of both molecules showed a marked increase in the number of blood vessels on embryonic day 14 in chicken embryos grown ex ovo, while, at the same time providing an antibiotic local concentration capable of inhibiting Staphylococcus aureus bacterial growth.
30654210	5	63	theme	release	942:948	arg1	function					1013:1020	a function	1011:1020	a function of the cephalexin loading strategy	1011:1055	In this sense, different release patterns, monitored by UV-spectroscopy, could be tailored as a function of the cephalexin loading strategy, either releasing all the loaded cephalexin in the first 4 h or less than 50% after 24 h.
30654210	5	63	theme	release	942:948	arg1	patterns					950:957	different release patterns	932:957	different release patterns	932:957	In this sense, different release patterns, monitored by UV-spectroscopy, could be tailored as a function of the cephalexin loading strategy, either releasing all the loaded cephalexin in the first 4 h or less than 50% after 24 h.
30654210	6	64	theme	high	1191:1194	arg1	hydrophilicity					1196:1209	a high hydrophilicity	1189:1209	a high hydrophilicity	1189:1209	The scaffold surface was characterized by a high hydrophilicity, with contact angles between 50° and 63°, which enabled the adhesion and proliferation of preosteoblastic cells.
30654210	10	65	theme	chicken	2040:2046	arg1	embryos					2048:2054	chicken embryos	2040:2054	chicken embryos grown ex ovo	2040:2067	These scaffolds, whose composition resembles the extracellular matrix are suitable for the adhesion of preosteoblast cells, exhibit a sustained cephalexin delivery adequate for inhibiting bacterial growth as well as release the proangiogenic molecule which induces blood vessel formation in chicken embryos grown ex ovo.
30654210	0	66	theme	proangiogenic	70:82	arg1	properties					102:111	proangiogenic and antibacterial properties	70:111	proangiogenic and antibacterial properties	70:111	Fabrication of a nanoparticle-containing 3D porous bone scaffold with proangiogenic and antibacterial properties.
30654210	8	67	with	scaffolds	1578:1586	arg1	porosity					1604:1611	a designed porosity	1593:1611	a designed porosity	1593:1611	In this article multifunctional 3D porous scaffolds with a designed porosity have been fabricated.
30654210	7	68	theme	SIGNIFICANCE	1337:1348	arg1	STATEMENT					1324:1332	STATEMENT	1324:1332	STATEMENT OF SIGNIFICANCE: The localized delivery of bioactive molecules has attracted significant attention due to the potential for dose reduction as well as reduced side effects compared to systemic delivery.	1324:1534	STATEMENT OF SIGNIFICANCE: The localized delivery of bioactive molecules has attracted significant attention due to the potential for dose reduction as well as reduced side effects compared to systemic delivery.
30654210	0	69	theme	antibacterial	88:100	arg1	properties					102:111	proangiogenic and antibacterial properties	70:111	proangiogenic and antibacterial properties	70:111	Fabrication of a nanoparticle-containing 3D porous bone scaffold with proangiogenic and antibacterial properties.
30654210	4	70	theme	vessels	726:732	arg1	number					710:715	the number	706:715	the number of blood vessels	706:732	The dual release of both molecules showed a marked increase in the number of blood vessels on embryonic day 14 in chicken embryos grown ex ovo, while, at the same time providing an antibiotic local concentration capable of inhibiting Staphylococcus aureus bacterial growth.
30654210	5	71	from	cephalexin	1090:1099	arg1	4 h					1114:1116	the first 4 h	1104:1116	the first 4 h	1104:1116	In this sense, different release patterns, monitored by UV-spectroscopy, could be tailored as a function of the cephalexin loading strategy, either releasing all the loaded cephalexin in the first 4 h or less than 50% after 24 h.
30654210	2	72	theme	shaping	307:313	arg1	technology					315:324	This shaping technology	302:324	This shaping technology	302:324	This shaping technology allows the introduction of thermally-labile molecules within the scaffolds during the fabrication procedure.
30654210	5	73	theme	first	1108:1112	arg1	4 h					1114:1116	the first 4 h	1104:1116	the first 4 h	1104:1116	In this sense, different release patterns, monitored by UV-spectroscopy, could be tailored as a function of the cephalexin loading strategy, either releasing all the loaded cephalexin in the first 4 h or less than 50% after 24 h.
30654210	4	74	theme	dual	647:650	arg1	release					652:658	The dual release	643:658	The dual release of both molecules	643:676	The dual release of both molecules showed a marked increase in the number of blood vessels on embryonic day 14 in chicken embryos grown ex ovo, while, at the same time providing an antibiotic local concentration capable of inhibiting Staphylococcus aureus bacterial growth.
30654210	4	75	theme	chicken	757:763	arg1	embryos					765:771	chicken embryos	757:771	chicken embryos grown ex ovo	757:784	The dual release of both molecules showed a marked increase in the number of blood vessels on embryonic day 14 in chicken embryos grown ex ovo, while, at the same time providing an antibiotic local concentration capable of inhibiting Staphylococcus aureus bacterial growth.
30654210	3	76	theme	Endothelial	467:477	arg1	Factor					486:491	Vascular Endothelial Growth Factor	458:491	Vascular Endothelial Growth Factor	458:491	An angiogenic protein, Vascular Endothelial Growth Factor, and an antibiotic, cephalexin, loaded in mesoporous silica nanoparticles, were included to design multifunctional scaffolds for bone reconstruction.
30654210	0	77	theme	nanoparticle-containing	17:39	arg1	scaffold					56:63	a nanoparticle-containing 3D porous bone scaffold	15:63	a nanoparticle-containing 3D porous bone scaffold	15:63	Fabrication of a nanoparticle-containing 3D porous bone scaffold with proangiogenic and antibacterial properties.
30654210	1	78	theme	3D	114:115	arg1	scaffolds					124:132	3D porous scaffolds	114:132	3D porous scaffolds based on agarose and nanocrystalline apatite, two structural components that act as a temporary mineralized extracellular matrix,	114:262	3D porous scaffolds based on agarose and nanocrystalline apatite, two structural components that act as a temporary mineralized extracellular matrix, were prepared by the GELPOR3D method.
30654210	8	79	theme	3D	1568:1569	arg1	scaffolds					1578:1586	multifunctional 3D porous scaffolds	1552:1586	multifunctional 3D porous scaffolds with a designed porosity	1552:1611	In this article multifunctional 3D porous scaffolds with a designed porosity have been fabricated.
30654210	0	80	theme	porous	44:49	arg1	scaffold					56:63	a nanoparticle-containing 3D porous bone scaffold	15:63	a nanoparticle-containing 3D porous bone scaffold	15:63	Fabrication of a nanoparticle-containing 3D porous bone scaffold with proangiogenic and antibacterial properties.
30654210	10	81	theme	cells	1866:1870	arg1	adhesion					1840:1847	the adhesion	1836:1847	the adhesion of preosteoblast cells	1836:1870	These scaffolds, whose composition resembles the extracellular matrix are suitable for the adhesion of preosteoblast cells, exhibit a sustained cephalexin delivery adequate for inhibiting bacterial growth as well as release the proangiogenic molecule which induces blood vessel formation in chicken embryos grown ex ovo.
30654210	0	82	theme	scaffold	56:63	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of a nanoparticle-containing 3D porous bone scaffold with proangiogenic and antibacterial properties.	0:112	Fabrication of a nanoparticle-containing 3D porous bone scaffold with proangiogenic and antibacterial properties.
30654210	5	83	theme	loading	1040:1046	arg1	strategy					1048:1055	the cephalexin loading strategy	1025:1055	the cephalexin loading strategy	1025:1055	In this sense, different release patterns, monitored by UV-spectroscopy, could be tailored as a function of the cephalexin loading strategy, either releasing all the loaded cephalexin in the first 4 h or less than 50% after 24 h.
30654210	3	84	theme	angiogenic	438:447	arg1	protein					449:455	An angiogenic protein	435:455	An angiogenic protein	435:455	An angiogenic protein, Vascular Endothelial Growth Factor, and an antibiotic, cephalexin, loaded in mesoporous silica nanoparticles, were included to design multifunctional scaffolds for bone reconstruction.
30654210	4	85	theme	antibiotic	824:833	arg1	concentration					841:853	an antibiotic local concentration	821:853	an antibiotic local concentration capable of inhibiting Staphylococcus aureus bacterial growth	821:914	The dual release of both molecules showed a marked increase in the number of blood vessels on embryonic day 14 in chicken embryos grown ex ovo, while, at the same time providing an antibiotic local concentration capable of inhibiting Staphylococcus aureus bacterial growth.
30654210	2	86	theme	thermally-labile	353:368	arg1	molecules					370:378	thermally-labile molecules	353:378	thermally-labile molecules	353:378	This shaping technology allows the introduction of thermally-labile molecules within the scaffolds during the fabrication procedure.
30654210	7	87	theme	bioactive	1377:1385	arg1	molecules					1387:1395	bioactive molecules	1377:1395	bioactive molecules	1377:1395	STATEMENT OF SIGNIFICANCE: The localized delivery of bioactive molecules has attracted significant attention due to the potential for dose reduction as well as reduced side effects compared to systemic delivery.
30654210	10	88	theme	sustained	1883:1891	arg1	delivery					1904:1911	a sustained cephalexin delivery	1881:1911	a sustained cephalexin delivery adequate for inhibiting bacterial growth as well as release the proangiogenic molecule which induces blood vessel formation in chicken embryos grown ex ovo	1881:2067	These scaffolds, whose composition resembles the extracellular matrix are suitable for the adhesion of preosteoblast cells, exhibit a sustained cephalexin delivery adequate for inhibiting bacterial growth as well as release the proangiogenic molecule which induces blood vessel formation in chicken embryos grown ex ovo.
30654210	4	89	from	day	747:749	arg1	embryos					765:771	chicken embryos	757:771	chicken embryos grown ex ovo	757:784	The dual release of both molecules showed a marked increase in the number of blood vessels on embryonic day 14 in chicken embryos grown ex ovo, while, at the same time providing an antibiotic local concentration capable of inhibiting Staphylococcus aureus bacterial growth.
30654210	1	90	theme	extracellular	242:254	arg1	matrix					256:261	a temporary mineralized extracellular matrix	218:261	a temporary mineralized extracellular matrix	218:261	3D porous scaffolds based on agarose and nanocrystalline apatite, two structural components that act as a temporary mineralized extracellular matrix, were prepared by the GELPOR3D method.
30654210	1	90	theme	extracellular	242:254	arg1	components					195:204	two structural components	180:204	two structural components that act as a temporary mineralized extracellular matrix	180:261	3D porous scaffolds based on agarose and nanocrystalline apatite, two structural components that act as a temporary mineralized extracellular matrix, were prepared by the GELPOR3D method.
30654210	10	91	theme	bacterial	1937:1945	arg1	growth					1947:1952	bacterial growth	1937:1952	bacterial growth as well as release the proangiogenic molecule which induces blood vessel formation in chicken embryos grown ex ovo	1937:2067	These scaffolds, whose composition resembles the extracellular matrix are suitable for the adhesion of preosteoblast cells, exhibit a sustained cephalexin delivery adequate for inhibiting bacterial growth as well as release the proangiogenic molecule which induces blood vessel formation in chicken embryos grown ex ovo.
30654210	4	92	theme	same	801:804	arg1	time					806:809	the same time	797:809	the same time	797:809	The dual release of both molecules showed a marked increase in the number of blood vessels on embryonic day 14 in chicken embryos grown ex ovo, while, at the same time providing an antibiotic local concentration capable of inhibiting Staphylococcus aureus bacterial growth.
30206169	7	0	from	biosynthesis	1458:1469	arg1	expression					1416:1425	the expression	1412:1425	the expression of genes in peptidoglycan (PG) biosynthesis, including the peptidoglycan-cross-linking transpeptidase genes ponA and dacC Consistent with this observation	1412:1580	An increase in the concentration of d-Ala in cells downregulated the expression of genes in peptidoglycan (PG) biosynthesis, including the peptidoglycan-cross-linking transpeptidase genes ponA and dacC Consistent with this observation, ultraperformance liquid chromatography-mass spectrometry analysis of murein from P. aeruginosa cells revealed that dadA deletion mutants contained PG with reduced cross-linking and altered composition compared to wild-type cells.IMPORTANCE The mechanical properties of bacteria are important for protecting cells against physical stress.
30206169	9	1	dep	pathogen	2263:2270	arg1	aeruginosa					2284:2293	Pseudomonas aeruginosa	2272:2293	the opportunistic human pathogen Pseudomonas aeruginosa This approach enabled us to discover proteins with roles in a diverse range of biochemical pathways that influence the stiffness of P. aeruginosa cells	2239:2445	Using a unique high-throughput biophysical assay, we identified genes coding proteins that modulate cell stiffness in the opportunistic human pathogen Pseudomonas aeruginosa This approach enabled us to discover proteins with roles in a diverse range of biochemical pathways that influence the stiffness of P. aeruginosa cells.
30206169	9	1	dep	pathogen	2263:2270	arg1	enabled					2309:2315	enabled	2309:2315	enabled us to discover proteins with roles in a diverse range of biochemical pathways that influence the stiffness of P. aeruginosa cells	2309:2445	Using a unique high-throughput biophysical assay, we identified genes coding proteins that modulate cell stiffness in the opportunistic human pathogen Pseudomonas aeruginosa This approach enabled us to discover proteins with roles in a diverse range of biochemical pathways that influence the stiffness of P. aeruginosa cells.
30206169	4	2	theme	individual	887:896	arg1	genes					898:902	P. aeruginosa individual genes	873:902	P. aeruginosa individual genes	873:902	We used a high-throughput technique to study a library of 5,790 loss-of-function mutants covering ~80% of the nonessential genes and correlated P. aeruginosa individual genes with cell stiffness.
30206169	7	3	dep	cells.IMPORTANCE	1806:1821	arg1	important					1865:1873	important	1865:1873	important	1865:1873	An increase in the concentration of d-Ala in cells downregulated the expression of genes in peptidoglycan (PG) biosynthesis, including the peptidoglycan-cross-linking transpeptidase genes ponA and dacC Consistent with this observation, ultraperformance liquid chromatography-mass spectrometry analysis of murein from P. aeruginosa cells revealed that dadA deletion mutants contained PG with reduced cross-linking and altered composition compared to wild-type cells.IMPORTANCE The mechanical properties of bacteria are important for protecting cells against physical stress.
30206169	7	3	dep	cells.IMPORTANCE	1806:1821	arg1	properties					1838:1847	The mechanical properties	1823:1847	The mechanical properties of bacteria	1823:1859	An increase in the concentration of d-Ala in cells downregulated the expression of genes in peptidoglycan (PG) biosynthesis, including the peptidoglycan-cross-linking transpeptidase genes ponA and dacC Consistent with this observation, ultraperformance liquid chromatography-mass spectrometry analysis of murein from P. aeruginosa cells revealed that dadA deletion mutants contained PG with reduced cross-linking and altered composition compared to wild-type cells.IMPORTANCE The mechanical properties of bacteria are important for protecting cells against physical stress.
30206169	2	4	theme	mechanical	289:298	arg1	properties					300:309	mechanical properties	289:309	mechanical properties of bacteria	289:321	Many successful antibiotic chemotherapies target elements that alter mechanical properties of bacteria, and yet a global view of the biochemistry underlying the regulation of bacterial cell stiffness is still emerging.
30206169	4	5	theme	genes	852:856	arg1	%					830:830	~80%	827:830	~80% of the nonessential genes	827:856	We used a high-throughput technique to study a library of 5,790 loss-of-function mutants covering ~80% of the nonessential genes and correlated P. aeruginosa individual genes with cell stiffness.
30206169	4	5	theme	genes	852:856	arg1	genes					852:856	the nonessential genes	835:856	the nonessential genes	835:856	We used a high-throughput technique to study a library of 5,790 loss-of-function mutants covering ~80% of the nonessential genes and correlated P. aeruginosa individual genes with cell stiffness.
30206169	7	6	theme	mechanical	1827:1836	arg1	important					1865:1873	important	1865:1873	important	1865:1873	An increase in the concentration of d-Ala in cells downregulated the expression of genes in peptidoglycan (PG) biosynthesis, including the peptidoglycan-cross-linking transpeptidase genes ponA and dacC Consistent with this observation, ultraperformance liquid chromatography-mass spectrometry analysis of murein from P. aeruginosa cells revealed that dadA deletion mutants contained PG with reduced cross-linking and altered composition compared to wild-type cells.IMPORTANCE The mechanical properties of bacteria are important for protecting cells against physical stress.
30206169	7	6	theme	mechanical	1827:1836	arg1	properties					1838:1847	The mechanical properties	1823:1847	The mechanical properties of bacteria	1823:1859	An increase in the concentration of d-Ala in cells downregulated the expression of genes in peptidoglycan (PG) biosynthesis, including the peptidoglycan-cross-linking transpeptidase genes ponA and dacC Consistent with this observation, ultraperformance liquid chromatography-mass spectrometry analysis of murein from P. aeruginosa cells revealed that dadA deletion mutants contained PG with reduced cross-linking and altered composition compared to wild-type cells.IMPORTANCE The mechanical properties of bacteria are important for protecting cells against physical stress.
30206169	11	7	theme	new	2875:2877	arg1	approaches					2879:2888	new approaches	2875:2888	new approaches	2875:2888	This research demonstrates that there is much to learn about mechanical regulation in bacteria, and these studies revealed new nonessential P. aeruginosa targets that may enhance antibacterial chemotherapies or lead to new approaches.
30206169	10	8	theme	machinery	2585:2593	arg1	expression					2571:2580	expression	2571:2580	expression of machinery that cross-links this material and decreases cell stiffness	2571:2653	We demonstrate that d-Ala-a component of the peptidoglycan-is tightly regulated in cells and that its accumulation reduces expression of machinery that cross-links this material and decreases cell stiffness.
30206169	3	9	from	pathogens	506:514	arg1	interesting					471:481	interesting	471:481	interesting	471:481	This connection is particularly interesting in opportunistic human pathogens such as Pseudomonas aeruginosa that have a large (80%) proportion of genes of unknown function and low susceptibility to different families of antibiotics, including beta-lactams, aminoglycosides, and quinolones.
30206169	1	10	theme	osmotic	181:187	arg1	pressure					189:196	the large osmotic pressure	171:196	the large osmotic pressure across the cell wall	171:217	The stiffness of bacteria prevents cells from bursting due to the large osmotic pressure across the cell wall.
30206169	7	11	from	expression	1416:1425	arg1	biosynthesis					1458:1469	peptidoglycan (PG) biosynthesis	1439:1469	peptidoglycan (PG) biosynthesis	1439:1469	An increase in the concentration of d-Ala in cells downregulated the expression of genes in peptidoglycan (PG) biosynthesis, including the peptidoglycan-cross-linking transpeptidase genes ponA and dacC Consistent with this observation, ultraperformance liquid chromatography-mass spectrometry analysis of murein from P. aeruginosa cells revealed that dadA deletion mutants contained PG with reduced cross-linking and altered composition compared to wild-type cells.IMPORTANCE The mechanical properties of bacteria are important for protecting cells against physical stress.
30206169	7	12	theme	murein	1652:1657	arg1	analysis					1640:1647	ultraperformance liquid chromatography-mass spectrometry analysis	1583:1647	ultraperformance liquid chromatography-mass spectrometry analysis of murein from P. aeruginosa cells	1583:1682	An increase in the concentration of d-Ala in cells downregulated the expression of genes in peptidoglycan (PG) biosynthesis, including the peptidoglycan-cross-linking transpeptidase genes ponA and dacC Consistent with this observation, ultraperformance liquid chromatography-mass spectrometry analysis of murein from P. aeruginosa cells revealed that dadA deletion mutants contained PG with reduced cross-linking and altered composition compared to wild-type cells.IMPORTANCE The mechanical properties of bacteria are important for protecting cells against physical stress.
30206169	5	13	with	proteins	959:966	arg1	functions					981:989	diverse functions	973:989	diverse functions that, when deleted individually, decreased cell stiffness by >20%	973:1055	We identified 42 genes coding for proteins with diverse functions that, when deleted individually, decreased cell stiffness by >20%.
30206169	9	14	theme	human	2257:2261	arg1	pathogen					2263:2270	the opportunistic human pathogen	2239:2270	the opportunistic human pathogen Pseudomonas aeruginosa This approach enabled us to discover proteins with roles in a diverse range of biochemical pathways that influence the stiffness of P. aeruginosa cells	2239:2445	Using a unique high-throughput biophysical assay, we identified genes coding proteins that modulate cell stiffness in the opportunistic human pathogen Pseudomonas aeruginosa This approach enabled us to discover proteins with roles in a diverse range of biochemical pathways that influence the stiffness of P. aeruginosa cells.
30206169	8	15	theme	cellular	1961:1968	arg1	element					1970:1976	the best-characterized cellular element	1938:1976	the best-characterized cellular element contributing to bacterial cell mechanics	1938:2017	The cell wall is the best-characterized cellular element contributing to bacterial cell mechanics; however, the biochemistry underlying its regulation and assembly is still not completely understood.
30206169	8	15	theme	cellular	1961:1968	arg1	wall					1930:1933	The cell wall	1921:1933	The cell wall	1921:1933	The cell wall is the best-characterized cellular element contributing to bacterial cell mechanics; however, the biochemistry underlying its regulation and assembly is still not completely understood.
30206169	7	16	from	genes	1430:1434	arg1	biosynthesis					1458:1469	peptidoglycan (PG) biosynthesis	1439:1469	peptidoglycan (PG) biosynthesis	1439:1469	An increase in the concentration of d-Ala in cells downregulated the expression of genes in peptidoglycan (PG) biosynthesis, including the peptidoglycan-cross-linking transpeptidase genes ponA and dacC Consistent with this observation, ultraperformance liquid chromatography-mass spectrometry analysis of murein from P. aeruginosa cells revealed that dadA deletion mutants contained PG with reduced cross-linking and altered composition compared to wild-type cells.IMPORTANCE The mechanical properties of bacteria are important for protecting cells against physical stress.
30206169	7	17	theme	altered	1764:1770	arg1	composition					1772:1782	altered composition	1764:1782	altered composition	1764:1782	An increase in the concentration of d-Ala in cells downregulated the expression of genes in peptidoglycan (PG) biosynthesis, including the peptidoglycan-cross-linking transpeptidase genes ponA and dacC Consistent with this observation, ultraperformance liquid chromatography-mass spectrometry analysis of murein from P. aeruginosa cells revealed that dadA deletion mutants contained PG with reduced cross-linking and altered composition compared to wild-type cells.IMPORTANCE The mechanical properties of bacteria are important for protecting cells against physical stress.
30206169	2	18	theme	Many	220:223	arg1	chemotherapies					247:260	Many successful antibiotic chemotherapies	220:260	Many successful antibiotic chemotherapies	220:260	Many successful antibiotic chemotherapies target elements that alter mechanical properties of bacteria, and yet a global view of the biochemistry underlying the regulation of bacterial cell stiffness is still emerging.
30206169	1	19	theme	cell	209:212	arg1	wall					214:217	the cell wall	205:217	the cell wall	205:217	The stiffness of bacteria prevents cells from bursting due to the large osmotic pressure across the cell wall.
30206169	7	20	theme	chromatography-mass	1607:1625	arg1	spectrometry					1627:1638	ultraperformance liquid chromatography-mass spectrometry	1583:1638	ultraperformance liquid chromatography-mass spectrometry analysis of murein from P. aeruginosa cells	1583:1682	An increase in the concentration of d-Ala in cells downregulated the expression of genes in peptidoglycan (PG) biosynthesis, including the peptidoglycan-cross-linking transpeptidase genes ponA and dacC Consistent with this observation, ultraperformance liquid chromatography-mass spectrometry analysis of murein from P. aeruginosa cells revealed that dadA deletion mutants contained PG with reduced cross-linking and altered composition compared to wild-type cells.IMPORTANCE The mechanical properties of bacteria are important for protecting cells against physical stress.
30206169	7	21	from	cells	1678:1682	arg1	analysis					1640:1647	ultraperformance liquid chromatography-mass spectrometry analysis	1583:1647	ultraperformance liquid chromatography-mass spectrometry analysis of murein from P. aeruginosa cells	1583:1682	An increase in the concentration of d-Ala in cells downregulated the expression of genes in peptidoglycan (PG) biosynthesis, including the peptidoglycan-cross-linking transpeptidase genes ponA and dacC Consistent with this observation, ultraperformance liquid chromatography-mass spectrometry analysis of murein from P. aeruginosa cells revealed that dadA deletion mutants contained PG with reduced cross-linking and altered composition compared to wild-type cells.IMPORTANCE The mechanical properties of bacteria are important for protecting cells against physical stress.
30206169	7	21	from	cells	1678:1682	arg1	murein					1652:1657	murein	1652:1657	murein from P. aeruginosa cells	1652:1682	An increase in the concentration of d-Ala in cells downregulated the expression of genes in peptidoglycan (PG) biosynthesis, including the peptidoglycan-cross-linking transpeptidase genes ponA and dacC Consistent with this observation, ultraperformance liquid chromatography-mass spectrometry analysis of murein from P. aeruginosa cells revealed that dadA deletion mutants contained PG with reduced cross-linking and altered composition compared to wild-type cells.IMPORTANCE The mechanical properties of bacteria are important for protecting cells against physical stress.
30206169	8	22	theme	bacterial	1994:2002	arg1	mechanics					2009:2017	bacterial cell mechanics	1994:2017	bacterial cell mechanics	1994:2017	The cell wall is the best-characterized cellular element contributing to bacterial cell mechanics; however, the biochemistry underlying its regulation and assembly is still not completely understood.
30206169	7	23	dep	genes	1529:1533	arg1	genes					1529:1533	the peptidoglycan-cross-linking transpeptidase genes	1482:1533	the peptidoglycan-cross-linking transpeptidase genes ponA and dacC Consistent with this observation	1482:1580	An increase in the concentration of d-Ala in cells downregulated the expression of genes in peptidoglycan (PG) biosynthesis, including the peptidoglycan-cross-linking transpeptidase genes ponA and dacC Consistent with this observation, ultraperformance liquid chromatography-mass spectrometry analysis of murein from P. aeruginosa cells revealed that dadA deletion mutants contained PG with reduced cross-linking and altered composition compared to wild-type cells.IMPORTANCE The mechanical properties of bacteria are important for protecting cells against physical stress.
30206169	7	23	dep	genes	1529:1533	arg1	dacC					1544:1547	dacC	1544:1547	dacC	1544:1547	An increase in the concentration of d-Ala in cells downregulated the expression of genes in peptidoglycan (PG) biosynthesis, including the peptidoglycan-cross-linking transpeptidase genes ponA and dacC Consistent with this observation, ultraperformance liquid chromatography-mass spectrometry analysis of murein from P. aeruginosa cells revealed that dadA deletion mutants contained PG with reduced cross-linking and altered composition compared to wild-type cells.IMPORTANCE The mechanical properties of bacteria are important for protecting cells against physical stress.
30206169	7	23	dep	genes	1529:1533	arg1	ponA					1535:1538	ponA	1535:1538	ponA	1535:1538	An increase in the concentration of d-Ala in cells downregulated the expression of genes in peptidoglycan (PG) biosynthesis, including the peptidoglycan-cross-linking transpeptidase genes ponA and dacC Consistent with this observation, ultraperformance liquid chromatography-mass spectrometry analysis of murein from P. aeruginosa cells revealed that dadA deletion mutants contained PG with reduced cross-linking and altered composition compared to wild-type cells.IMPORTANCE The mechanical properties of bacteria are important for protecting cells against physical stress.
30206169	3	24	theme	genes	585:589	arg1	%					568:568	80%	566:568	80%	566:568	This connection is particularly interesting in opportunistic human pathogens such as Pseudomonas aeruginosa that have a large (80%) proportion of genes of unknown function and low susceptibility to different families of antibiotics, including beta-lactams, aminoglycosides, and quinolones.
30206169	3	24	theme	genes	585:589	arg1	proportion					571:580	a large (80%) proportion	557:580	a large (80%) proportion of genes of unknown function and low susceptibility to different families of antibiotics, including beta-lactams, aminoglycosides, and quinolones	557:726	This connection is particularly interesting in opportunistic human pathogens such as Pseudomonas aeruginosa that have a large (80%) proportion of genes of unknown function and low susceptibility to different families of antibiotics, including beta-lactams, aminoglycosides, and quinolones.
30206169	7	25	from	increase	1350:1357	arg1	concentration					1366:1378	the concentration	1362:1378	the concentration of d-Ala in cells	1362:1396	An increase in the concentration of d-Ala in cells downregulated the expression of genes in peptidoglycan (PG) biosynthesis, including the peptidoglycan-cross-linking transpeptidase genes ponA and dacC Consistent with this observation, ultraperformance liquid chromatography-mass spectrometry analysis of murein from P. aeruginosa cells revealed that dadA deletion mutants contained PG with reduced cross-linking and altered composition compared to wild-type cells.IMPORTANCE The mechanical properties of bacteria are important for protecting cells against physical stress.
30206169	7	26	theme	ultraperformance	1583:1598	arg1	spectrometry					1627:1638	ultraperformance liquid chromatography-mass spectrometry	1583:1638	ultraperformance liquid chromatography-mass spectrometry analysis of murein from P. aeruginosa cells	1583:1682	An increase in the concentration of d-Ala in cells downregulated the expression of genes in peptidoglycan (PG) biosynthesis, including the peptidoglycan-cross-linking transpeptidase genes ponA and dacC Consistent with this observation, ultraperformance liquid chromatography-mass spectrometry analysis of murein from P. aeruginosa cells revealed that dadA deletion mutants contained PG with reduced cross-linking and altered composition compared to wild-type cells.IMPORTANCE The mechanical properties of bacteria are important for protecting cells against physical stress.
30206169	11	27	theme	P. aeruginosa	2796:2808	arg1	targets					2810:2816	new nonessential P. aeruginosa targets	2779:2816	new nonessential P. aeruginosa targets that may enhance antibacterial chemotherapies or lead to new approaches	2779:2888	This research demonstrates that there is much to learn about mechanical regulation in bacteria, and these studies revealed new nonessential P. aeruginosa targets that may enhance antibacterial chemotherapies or lead to new approaches.
30206169	6	28	theme	cell	1269:1272	arg1	stiffness					1274:1282	cell stiffness	1269:1282	cell stiffness	1269:1282	This approach enabled us to construct a "mechanical genome" for P. aeruginosa d-Alanine dehydrogenase (DadA) is an enzyme that converts d-Ala to pyruvate that was included among the hits; when DadA was deleted, cell stiffness decreased by 18% (using multiple assays to measure mechanics).
30206169	3	29	theme	susceptibility	619:632	arg1	aminoglycosides					696:710	aminoglycosides	696:710	aminoglycosides	696:710	This connection is particularly interesting in opportunistic human pathogens such as Pseudomonas aeruginosa that have a large (80%) proportion of genes of unknown function and low susceptibility to different families of antibiotics, including beta-lactams, aminoglycosides, and quinolones.
30206169	3	29	theme	susceptibility	619:632	arg1	genes					585:589	genes	585:589	genes	585:589	This connection is particularly interesting in opportunistic human pathogens such as Pseudomonas aeruginosa that have a large (80%) proportion of genes of unknown function and low susceptibility to different families of antibiotics, including beta-lactams, aminoglycosides, and quinolones.
30206169	3	29	theme	susceptibility	619:632	arg1	quinolones					717:726	quinolones	717:726	quinolones	717:726	This connection is particularly interesting in opportunistic human pathogens such as Pseudomonas aeruginosa that have a large (80%) proportion of genes of unknown function and low susceptibility to different families of antibiotics, including beta-lactams, aminoglycosides, and quinolones.
30206169	3	29	theme	susceptibility	619:632	arg1	beta-lactams					682:693	beta-lactams	682:693	beta-lactams	682:693	This connection is particularly interesting in opportunistic human pathogens such as Pseudomonas aeruginosa that have a large (80%) proportion of genes of unknown function and low susceptibility to different families of antibiotics, including beta-lactams, aminoglycosides, and quinolones.
30206169	7	30	with	Consistent	1549:1558	arg1	observation					1570:1580	this observation	1565:1580	this observation	1565:1580	An increase in the concentration of d-Ala in cells downregulated the expression of genes in peptidoglycan (PG) biosynthesis, including the peptidoglycan-cross-linking transpeptidase genes ponA and dacC Consistent with this observation, ultraperformance liquid chromatography-mass spectrometry analysis of murein from P. aeruginosa cells revealed that dadA deletion mutants contained PG with reduced cross-linking and altered composition compared to wild-type cells.IMPORTANCE The mechanical properties of bacteria are important for protecting cells against physical stress.
30206169	0	31	theme	Metabolism	56:65	arg1	Role					38:41	the Role	34:41	the Role of d-Alanine Metabolism in Pseudomonas aeruginosa Cell Stiffness	34:106	Mechanical Genomic Studies Reveal the Role of d-Alanine Metabolism in Pseudomonas aeruginosa Cell Stiffness.
30206169	2	32	theme	antibiotic	236:245	arg1	chemotherapies					247:260	Many successful antibiotic chemotherapies	220:260	Many successful antibiotic chemotherapies	220:260	Many successful antibiotic chemotherapies target elements that alter mechanical properties of bacteria, and yet a global view of the biochemistry underlying the regulation of bacterial cell stiffness is still emerging.
30206169	10	33	theme	d-Ala-a	2468:2474	arg1	component					2476:2484	d-Ala-a component	2468:2484	d-Ala-a component of the peptidoglycan-is	2468:2508	We demonstrate that d-Ala-a component of the peptidoglycan-is tightly regulated in cells and that its accumulation reduces expression of machinery that cross-links this material and decreases cell stiffness.
30206169	0	34	theme	Pseudomonas	70:80	arg1	Stiffness					98:106	Pseudomonas aeruginosa Cell Stiffness	70:106	Pseudomonas aeruginosa Cell Stiffness	70:106	Mechanical Genomic Studies Reveal the Role of d-Alanine Metabolism in Pseudomonas aeruginosa Cell Stiffness.
30206169	0	35	theme	Cell	93:96	arg1	Stiffness					98:106	Pseudomonas aeruginosa Cell Stiffness	70:106	Pseudomonas aeruginosa Cell Stiffness	70:106	Mechanical Genomic Studies Reveal the Role of d-Alanine Metabolism in Pseudomonas aeruginosa Cell Stiffness.
30206169	7	36	theme	deletion	1703:1710	arg1	mutants					1712:1718	dadA deletion mutants	1698:1718	dadA deletion mutants	1698:1718	An increase in the concentration of d-Ala in cells downregulated the expression of genes in peptidoglycan (PG) biosynthesis, including the peptidoglycan-cross-linking transpeptidase genes ponA and dacC Consistent with this observation, ultraperformance liquid chromatography-mass spectrometry analysis of murein from P. aeruginosa cells revealed that dadA deletion mutants contained PG with reduced cross-linking and altered composition compared to wild-type cells.IMPORTANCE The mechanical properties of bacteria are important for protecting cells against physical stress.
30206169	10	37	theme	peptidoglycan-is	2493:2508	arg1	component					2476:2484	d-Ala-a component	2468:2484	d-Ala-a component of the peptidoglycan-is	2468:2508	We demonstrate that d-Ala-a component of the peptidoglycan-is tightly regulated in cells and that its accumulation reduces expression of machinery that cross-links this material and decreases cell stiffness.
30206169	2	38	theme	bacterial	395:403	arg1	stiffness					410:418	bacterial cell stiffness	395:418	bacterial cell stiffness	395:418	Many successful antibiotic chemotherapies target elements that alter mechanical properties of bacteria, and yet a global view of the biochemistry underlying the regulation of bacterial cell stiffness is still emerging.
30206169	6	39	theme	multiple	1308:1315	arg1	assays					1317:1322	multiple assays	1308:1322	multiple assays to measure mechanics	1308:1343	This approach enabled us to construct a "mechanical genome" for P. aeruginosa d-Alanine dehydrogenase (DadA) is an enzyme that converts d-Ala to pyruvate that was included among the hits; when DadA was deleted, cell stiffness decreased by 18% (using multiple assays to measure mechanics).
30206169	7	40	from	analysis	1640:1647	arg1	cells					1678:1682	P. aeruginosa cells	1664:1682	P. aeruginosa cells	1664:1682	An increase in the concentration of d-Ala in cells downregulated the expression of genes in peptidoglycan (PG) biosynthesis, including the peptidoglycan-cross-linking transpeptidase genes ponA and dacC Consistent with this observation, ultraperformance liquid chromatography-mass spectrometry analysis of murein from P. aeruginosa cells revealed that dadA deletion mutants contained PG with reduced cross-linking and altered composition compared to wild-type cells.IMPORTANCE The mechanical properties of bacteria are important for protecting cells against physical stress.
30206169	4	41	theme	high-throughput	739:753	arg1	technique					755:763	a high-throughput technique	737:763	a high-throughput technique	737:763	We used a high-throughput technique to study a library of 5,790 loss-of-function mutants covering ~80% of the nonessential genes and correlated P. aeruginosa individual genes with cell stiffness.
30206169	0	42	theme	Mechanical	0:9	arg1	Studies					19:25	Mechanical Genomic Studies	0:25	Mechanical Genomic Studies	0:25	Mechanical Genomic Studies Reveal the Role of d-Alanine Metabolism in Pseudomonas aeruginosa Cell Stiffness.
30206169	0	43	dep	Pseudomonas	70:80	arg1	aeruginosa					82:91	aeruginosa	82:91	aeruginosa	82:91	Mechanical Genomic Studies Reveal the Role of d-Alanine Metabolism in Pseudomonas aeruginosa Cell Stiffness.
30206169	9	44	theme	biochemical	2374:2384	arg1	pathways					2386:2393	biochemical pathways	2374:2393	biochemical pathways that influence the stiffness of P. aeruginosa cells	2374:2445	Using a unique high-throughput biophysical assay, we identified genes coding proteins that modulate cell stiffness in the opportunistic human pathogen Pseudomonas aeruginosa This approach enabled us to discover proteins with roles in a diverse range of biochemical pathways that influence the stiffness of P. aeruginosa cells.
30206169	7	45	theme	peptidoglycan-cross-linking	1486:1512	arg1	genes					1529:1533	the peptidoglycan-cross-linking transpeptidase genes	1482:1533	the peptidoglycan-cross-linking transpeptidase genes ponA and dacC Consistent with this observation	1482:1580	An increase in the concentration of d-Ala in cells downregulated the expression of genes in peptidoglycan (PG) biosynthesis, including the peptidoglycan-cross-linking transpeptidase genes ponA and dacC Consistent with this observation, ultraperformance liquid chromatography-mass spectrometry analysis of murein from P. aeruginosa cells revealed that dadA deletion mutants contained PG with reduced cross-linking and altered composition compared to wild-type cells.IMPORTANCE The mechanical properties of bacteria are important for protecting cells against physical stress.
30206169	7	45	theme	peptidoglycan-cross-linking	1486:1512	arg1	dacC					1544:1547	dacC	1544:1547	dacC	1544:1547	An increase in the concentration of d-Ala in cells downregulated the expression of genes in peptidoglycan (PG) biosynthesis, including the peptidoglycan-cross-linking transpeptidase genes ponA and dacC Consistent with this observation, ultraperformance liquid chromatography-mass spectrometry analysis of murein from P. aeruginosa cells revealed that dadA deletion mutants contained PG with reduced cross-linking and altered composition compared to wild-type cells.IMPORTANCE The mechanical properties of bacteria are important for protecting cells against physical stress.
30206169	7	45	theme	peptidoglycan-cross-linking	1486:1512	arg1	ponA					1535:1538	ponA	1535:1538	ponA	1535:1538	An increase in the concentration of d-Ala in cells downregulated the expression of genes in peptidoglycan (PG) biosynthesis, including the peptidoglycan-cross-linking transpeptidase genes ponA and dacC Consistent with this observation, ultraperformance liquid chromatography-mass spectrometry analysis of murein from P. aeruginosa cells revealed that dadA deletion mutants contained PG with reduced cross-linking and altered composition compared to wild-type cells.IMPORTANCE The mechanical properties of bacteria are important for protecting cells against physical stress.
30206169	5	46	theme	diverse	973:979	arg1	functions					981:989	diverse functions	973:989	diverse functions that, when deleted individually, decreased cell stiffness by >20%	973:1055	We identified 42 genes coding for proteins with diverse functions that, when deleted individually, decreased cell stiffness by >20%.
30206169	9	47	theme	unique	2129:2134	arg1	assay					2164:2168	a unique high-throughput biophysical assay	2127:2168	a unique high-throughput biophysical assay	2127:2168	Using a unique high-throughput biophysical assay, we identified genes coding proteins that modulate cell stiffness in the opportunistic human pathogen Pseudomonas aeruginosa This approach enabled us to discover proteins with roles in a diverse range of biochemical pathways that influence the stiffness of P. aeruginosa cells.
30206169	2	48	theme	stiffness	410:418	arg1	regulation					381:390	the regulation	377:390	the regulation of bacterial cell stiffness	377:418	Many successful antibiotic chemotherapies target elements that alter mechanical properties of bacteria, and yet a global view of the biochemistry underlying the regulation of bacterial cell stiffness is still emerging.
30206169	3	49	contain	have	552:555	arg2	proportion					571:580	a large (80%) proportion	557:580	a large (80%) proportion of genes of unknown function and low susceptibility to different families of antibiotics, including beta-lactams, aminoglycosides, and quinolones	557:726	This connection is particularly interesting in opportunistic human pathogens such as Pseudomonas aeruginosa that have a large (80%) proportion of genes of unknown function and low susceptibility to different families of antibiotics, including beta-lactams, aminoglycosides, and quinolones.
30206169	3	49	contain	have	552:555	arg2	%					568:568	80%	566:568	80%	566:568	This connection is particularly interesting in opportunistic human pathogens such as Pseudomonas aeruginosa that have a large (80%) proportion of genes of unknown function and low susceptibility to different families of antibiotics, including beta-lactams, aminoglycosides, and quinolones.
30206169	3	49	contain	have	552:555	arg1	aeruginosa					536:545	Pseudomonas aeruginosa	524:545	Pseudomonas aeruginosa that have a large (80%) proportion of genes of unknown function and low susceptibility to different families of antibiotics, including beta-lactams, aminoglycosides, and quinolones	524:726	This connection is particularly interesting in opportunistic human pathogens such as Pseudomonas aeruginosa that have a large (80%) proportion of genes of unknown function and low susceptibility to different families of antibiotics, including beta-lactams, aminoglycosides, and quinolones.
30206169	3	50	theme	different	637:645	arg1	families					647:654	different families	637:654	different families of antibiotics	637:669	This connection is particularly interesting in opportunistic human pathogens such as Pseudomonas aeruginosa that have a large (80%) proportion of genes of unknown function and low susceptibility to different families of antibiotics, including beta-lactams, aminoglycosides, and quinolones.
30206169	6	51	theme	P. aeruginosa	1122:1134	arg1	DadA					1161:1164	DadA	1161:1164	DadA	1161:1164	This approach enabled us to construct a "mechanical genome" for P. aeruginosa d-Alanine dehydrogenase (DadA) is an enzyme that converts d-Ala to pyruvate that was included among the hits; when DadA was deleted, cell stiffness decreased by 18% (using multiple assays to measure mechanics).
30206169	6	51	theme	P. aeruginosa	1122:1134	arg1	dehydrogenase					1146:1158	P. aeruginosa d-Alanine dehydrogenase	1122:1158	P. aeruginosa d-Alanine dehydrogenase (DadA) is an enzyme that converts d-Ala to pyruvate that was included among the hits	1122:1243	This approach enabled us to construct a "mechanical genome" for P. aeruginosa d-Alanine dehydrogenase (DadA) is an enzyme that converts d-Ala to pyruvate that was included among the hits; when DadA was deleted, cell stiffness decreased by 18% (using multiple assays to measure mechanics).
30206169	4	52	theme	loss-of-function	793:808	arg1	mutants					810:816	5,790 loss-of-function mutants	787:816	5,790 loss-of-function mutants covering ~80% of the nonessential genes	787:856	We used a high-throughput technique to study a library of 5,790 loss-of-function mutants covering ~80% of the nonessential genes and correlated P. aeruginosa individual genes with cell stiffness.
30206169	3	53	theme	opportunistic	486:498	arg1	pathogens					506:514	opportunistic human pathogens	486:514	opportunistic human pathogens such as Pseudomonas aeruginosa that have a large (80%) proportion of genes of unknown function and low susceptibility to different families of antibiotics, including beta-lactams, aminoglycosides, and quinolones	486:726	This connection is particularly interesting in opportunistic human pathogens such as Pseudomonas aeruginosa that have a large (80%) proportion of genes of unknown function and low susceptibility to different families of antibiotics, including beta-lactams, aminoglycosides, and quinolones.
30206169	3	53	theme	opportunistic	486:498	arg1	aeruginosa					536:545	Pseudomonas aeruginosa	524:545	Pseudomonas aeruginosa that have a large (80%) proportion of genes of unknown function and low susceptibility to different families of antibiotics, including beta-lactams, aminoglycosides, and quinolones	524:726	This connection is particularly interesting in opportunistic human pathogens such as Pseudomonas aeruginosa that have a large (80%) proportion of genes of unknown function and low susceptibility to different families of antibiotics, including beta-lactams, aminoglycosides, and quinolones.
30206169	7	54	theme	PG	1454:1455	arg1	biosynthesis					1458:1469	peptidoglycan (PG) biosynthesis	1439:1469	peptidoglycan (PG) biosynthesis	1439:1469	An increase in the concentration of d-Ala in cells downregulated the expression of genes in peptidoglycan (PG) biosynthesis, including the peptidoglycan-cross-linking transpeptidase genes ponA and dacC Consistent with this observation, ultraperformance liquid chromatography-mass spectrometry analysis of murein from P. aeruginosa cells revealed that dadA deletion mutants contained PG with reduced cross-linking and altered composition compared to wild-type cells.IMPORTANCE The mechanical properties of bacteria are important for protecting cells against physical stress.
30206169	9	55	theme	cells	2441:2445	arg1	stiffness					2414:2422	the stiffness	2410:2422	the stiffness of P. aeruginosa cells	2410:2445	Using a unique high-throughput biophysical assay, we identified genes coding proteins that modulate cell stiffness in the opportunistic human pathogen Pseudomonas aeruginosa This approach enabled us to discover proteins with roles in a diverse range of biochemical pathways that influence the stiffness of P. aeruginosa cells.
30206169	7	56	theme	peptidoglycan	1439:1451	arg1	biosynthesis					1458:1469	peptidoglycan (PG) biosynthesis	1439:1469	peptidoglycan (PG) biosynthesis	1439:1469	An increase in the concentration of d-Ala in cells downregulated the expression of genes in peptidoglycan (PG) biosynthesis, including the peptidoglycan-cross-linking transpeptidase genes ponA and dacC Consistent with this observation, ultraperformance liquid chromatography-mass spectrometry analysis of murein from P. aeruginosa cells revealed that dadA deletion mutants contained PG with reduced cross-linking and altered composition compared to wild-type cells.IMPORTANCE The mechanical properties of bacteria are important for protecting cells against physical stress.
30206169	7	57	theme	bacteria	1852:1859	arg1	important					1865:1873	important	1865:1873	important	1865:1873	An increase in the concentration of d-Ala in cells downregulated the expression of genes in peptidoglycan (PG) biosynthesis, including the peptidoglycan-cross-linking transpeptidase genes ponA and dacC Consistent with this observation, ultraperformance liquid chromatography-mass spectrometry analysis of murein from P. aeruginosa cells revealed that dadA deletion mutants contained PG with reduced cross-linking and altered composition compared to wild-type cells.IMPORTANCE The mechanical properties of bacteria are important for protecting cells against physical stress.
30206169	7	57	theme	bacteria	1852:1859	arg1	properties					1838:1847	The mechanical properties	1823:1847	The mechanical properties of bacteria	1823:1859	An increase in the concentration of d-Ala in cells downregulated the expression of genes in peptidoglycan (PG) biosynthesis, including the peptidoglycan-cross-linking transpeptidase genes ponA and dacC Consistent with this observation, ultraperformance liquid chromatography-mass spectrometry analysis of murein from P. aeruginosa cells revealed that dadA deletion mutants contained PG with reduced cross-linking and altered composition compared to wild-type cells.IMPORTANCE The mechanical properties of bacteria are important for protecting cells against physical stress.
30206169	6	58	theme	mechanical	1099:1108	arg1	"					1116:1116	a "mechanical genome"	1096:1116	a "mechanical genome" for P. aeruginosa d-Alanine dehydrogenase (DadA) is an enzyme that converts d-Ala to pyruvate that was included among the hits	1096:1243	This approach enabled us to construct a "mechanical genome" for P. aeruginosa d-Alanine dehydrogenase (DadA) is an enzyme that converts d-Ala to pyruvate that was included among the hits; when DadA was deleted, cell stiffness decreased by 18% (using multiple assays to measure mechanics).
30206169	11	59	theme	nonessential	2783:2794	arg1	targets					2810:2816	new nonessential P. aeruginosa targets	2779:2816	new nonessential P. aeruginosa targets that may enhance antibacterial chemotherapies or lead to new approaches	2779:2888	This research demonstrates that there is much to learn about mechanical regulation in bacteria, and these studies revealed new nonessential P. aeruginosa targets that may enhance antibacterial chemotherapies or lead to new approaches.
30206169	9	60	theme	coding	2191:2196	arg1	proteins					2198:2205	genes coding proteins	2185:2205	genes coding proteins that modulate cell stiffness in the opportunistic human pathogen Pseudomonas aeruginosa This approach enabled us to discover proteins with roles in a diverse range of biochemical pathways that influence the stiffness of P. aeruginosa cells	2185:2445	Using a unique high-throughput biophysical assay, we identified genes coding proteins that modulate cell stiffness in the opportunistic human pathogen Pseudomonas aeruginosa This approach enabled us to discover proteins with roles in a diverse range of biochemical pathways that influence the stiffness of P. aeruginosa cells.
30206169	7	61	theme	genes	1430:1434	arg1	expression					1416:1425	the expression	1412:1425	the expression of genes in peptidoglycan (PG) biosynthesis, including the peptidoglycan-cross-linking transpeptidase genes ponA and dacC Consistent with this observation	1412:1580	An increase in the concentration of d-Ala in cells downregulated the expression of genes in peptidoglycan (PG) biosynthesis, including the peptidoglycan-cross-linking transpeptidase genes ponA and dacC Consistent with this observation, ultraperformance liquid chromatography-mass spectrometry analysis of murein from P. aeruginosa cells revealed that dadA deletion mutants contained PG with reduced cross-linking and altered composition compared to wild-type cells.IMPORTANCE The mechanical properties of bacteria are important for protecting cells against physical stress.
30206169	4	62	theme	P. aeruginosa	873:885	arg1	genes					898:902	P. aeruginosa individual genes	873:902	P. aeruginosa individual genes	873:902	We used a high-throughput technique to study a library of 5,790 loss-of-function mutants covering ~80% of the nonessential genes and correlated P. aeruginosa individual genes with cell stiffness.
30206169	9	63	theme	biophysical	2152:2162	arg1	assay					2164:2168	a unique high-throughput biophysical assay	2127:2168	a unique high-throughput biophysical assay	2127:2168	Using a unique high-throughput biophysical assay, we identified genes coding proteins that modulate cell stiffness in the opportunistic human pathogen Pseudomonas aeruginosa This approach enabled us to discover proteins with roles in a diverse range of biochemical pathways that influence the stiffness of P. aeruginosa cells.
30206169	8	64	theme	cell	1925:1928	arg1	element					1970:1976	the best-characterized cellular element	1938:1976	the best-characterized cellular element contributing to bacterial cell mechanics	1938:2017	The cell wall is the best-characterized cellular element contributing to bacterial cell mechanics; however, the biochemistry underlying its regulation and assembly is still not completely understood.
30206169	8	64	theme	cell	1925:1928	arg1	wall					1930:1933	The cell wall	1921:1933	The cell wall	1921:1933	The cell wall is the best-characterized cellular element contributing to bacterial cell mechanics; however, the biochemistry underlying its regulation and assembly is still not completely understood.
30206169	3	65	theme	large	559:563	arg1	%					568:568	80%	566:568	80%	566:568	This connection is particularly interesting in opportunistic human pathogens such as Pseudomonas aeruginosa that have a large (80%) proportion of genes of unknown function and low susceptibility to different families of antibiotics, including beta-lactams, aminoglycosides, and quinolones.
30206169	3	65	theme	large	559:563	arg1	proportion					571:580	a large (80%) proportion	557:580	a large (80%) proportion of genes of unknown function and low susceptibility to different families of antibiotics, including beta-lactams, aminoglycosides, and quinolones	557:726	This connection is particularly interesting in opportunistic human pathogens such as Pseudomonas aeruginosa that have a large (80%) proportion of genes of unknown function and low susceptibility to different families of antibiotics, including beta-lactams, aminoglycosides, and quinolones.
30206169	11	66	theme	mechanical	2717:2726	arg1	regulation					2728:2737	mechanical regulation	2717:2737	mechanical regulation in bacteria	2717:2749	This research demonstrates that there is much to learn about mechanical regulation in bacteria, and these studies revealed new nonessential P. aeruginosa targets that may enhance antibacterial chemotherapies or lead to new approaches.
30206169	9	67	theme	cell	2221:2224	arg1	stiffness					2226:2234	cell stiffness	2221:2234	cell stiffness	2221:2234	Using a unique high-throughput biophysical assay, we identified genes coding proteins that modulate cell stiffness in the opportunistic human pathogen Pseudomonas aeruginosa This approach enabled us to discover proteins with roles in a diverse range of biochemical pathways that influence the stiffness of P. aeruginosa cells.
30206169	4	68	theme	nonessential	839:850	arg1	genes					852:856	the nonessential genes	835:856	the nonessential genes	835:856	We used a high-throughput technique to study a library of 5,790 loss-of-function mutants covering ~80% of the nonessential genes and correlated P. aeruginosa individual genes with cell stiffness.
30206169	7	69	theme	wild-type	1796:1804	arg1	cells.IMPORTANCE					1806:1821	wild-type cells.IMPORTANCE	1796:1821	wild-type cells.IMPORTANCE The mechanical properties of bacteria are important for protecting cells against physical stress	1796:1918	An increase in the concentration of d-Ala in cells downregulated the expression of genes in peptidoglycan (PG) biosynthesis, including the peptidoglycan-cross-linking transpeptidase genes ponA and dacC Consistent with this observation, ultraperformance liquid chromatography-mass spectrometry analysis of murein from P. aeruginosa cells revealed that dadA deletion mutants contained PG with reduced cross-linking and altered composition compared to wild-type cells.IMPORTANCE The mechanical properties of bacteria are important for protecting cells against physical stress.
30206169	7	70	theme	d-Ala	1383:1387	arg1	concentration					1366:1378	the concentration	1362:1378	the concentration of d-Ala in cells	1362:1396	An increase in the concentration of d-Ala in cells downregulated the expression of genes in peptidoglycan (PG) biosynthesis, including the peptidoglycan-cross-linking transpeptidase genes ponA and dacC Consistent with this observation, ultraperformance liquid chromatography-mass spectrometry analysis of murein from P. aeruginosa cells revealed that dadA deletion mutants contained PG with reduced cross-linking and altered composition compared to wild-type cells.IMPORTANCE The mechanical properties of bacteria are important for protecting cells against physical stress.
30206169	6	71	dep	dehydrogenase	1146:1158	arg1	enzyme					1173:1178	an enzyme	1170:1178	an enzyme that converts d-Ala to pyruvate that was included among the hits	1170:1243	This approach enabled us to construct a "mechanical genome" for P. aeruginosa d-Alanine dehydrogenase (DadA) is an enzyme that converts d-Ala to pyruvate that was included among the hits; when DadA was deleted, cell stiffness decreased by 18% (using multiple assays to measure mechanics).
30206169	8	72	theme	best-characterized	1942:1959	arg1	element					1970:1976	the best-characterized cellular element	1938:1976	the best-characterized cellular element contributing to bacterial cell mechanics	1938:2017	The cell wall is the best-characterized cellular element contributing to bacterial cell mechanics; however, the biochemistry underlying its regulation and assembly is still not completely understood.
30206169	8	72	theme	best-characterized	1942:1959	arg1	wall					1930:1933	The cell wall	1921:1933	The cell wall	1921:1933	The cell wall is the best-characterized cellular element contributing to bacterial cell mechanics; however, the biochemistry underlying its regulation and assembly is still not completely understood.
30206169	1	73	theme	large	175:179	arg1	pressure					189:196	the large osmotic pressure	171:196	the large osmotic pressure across the cell wall	171:217	The stiffness of bacteria prevents cells from bursting due to the large osmotic pressure across the cell wall.
30206169	9	74	theme	opportunistic	2243:2255	arg1	pathogen					2263:2270	the opportunistic human pathogen	2239:2270	the opportunistic human pathogen Pseudomonas aeruginosa This approach enabled us to discover proteins with roles in a diverse range of biochemical pathways that influence the stiffness of P. aeruginosa cells	2239:2445	Using a unique high-throughput biophysical assay, we identified genes coding proteins that modulate cell stiffness in the opportunistic human pathogen Pseudomonas aeruginosa This approach enabled us to discover proteins with roles in a diverse range of biochemical pathways that influence the stiffness of P. aeruginosa cells.
30206169	2	75	theme	bacteria	314:321	arg1	properties					300:309	mechanical properties	289:309	mechanical properties of bacteria	289:321	Many successful antibiotic chemotherapies target elements that alter mechanical properties of bacteria, and yet a global view of the biochemistry underlying the regulation of bacterial cell stiffness is still emerging.
30206169	7	76	theme	spectrometry	1627:1638	arg1	analysis					1640:1647	ultraperformance liquid chromatography-mass spectrometry analysis	1583:1647	ultraperformance liquid chromatography-mass spectrometry analysis of murein from P. aeruginosa cells	1583:1682	An increase in the concentration of d-Ala in cells downregulated the expression of genes in peptidoglycan (PG) biosynthesis, including the peptidoglycan-cross-linking transpeptidase genes ponA and dacC Consistent with this observation, ultraperformance liquid chromatography-mass spectrometry analysis of murein from P. aeruginosa cells revealed that dadA deletion mutants contained PG with reduced cross-linking and altered composition compared to wild-type cells.IMPORTANCE The mechanical properties of bacteria are important for protecting cells against physical stress.
30206169	5	77	dep	decreased	1024:1032	arg1	functions					981:989	diverse functions	973:989	diverse functions that, when deleted individually, decreased cell stiffness by >20%	973:1055	We identified 42 genes coding for proteins with diverse functions that, when deleted individually, decreased cell stiffness by >20%.
30206169	2	78	theme	successful	225:234	arg1	chemotherapies					247:260	Many successful antibiotic chemotherapies	220:260	Many successful antibiotic chemotherapies	220:260	Many successful antibiotic chemotherapies target elements that alter mechanical properties of bacteria, and yet a global view of the biochemistry underlying the regulation of bacterial cell stiffness is still emerging.
30206169	7	79	theme	liquid	1600:1605	arg1	spectrometry					1627:1638	ultraperformance liquid chromatography-mass spectrometry	1583:1638	ultraperformance liquid chromatography-mass spectrometry analysis of murein from P. aeruginosa cells	1583:1682	An increase in the concentration of d-Ala in cells downregulated the expression of genes in peptidoglycan (PG) biosynthesis, including the peptidoglycan-cross-linking transpeptidase genes ponA and dacC Consistent with this observation, ultraperformance liquid chromatography-mass spectrometry analysis of murein from P. aeruginosa cells revealed that dadA deletion mutants contained PG with reduced cross-linking and altered composition compared to wild-type cells.IMPORTANCE The mechanical properties of bacteria are important for protecting cells against physical stress.
30206169	8	80	theme	cell	2004:2007	arg1	mechanics					2009:2017	bacterial cell mechanics	1994:2017	bacterial cell mechanics	1994:2017	The cell wall is the best-characterized cellular element contributing to bacterial cell mechanics; however, the biochemistry underlying its regulation and assembly is still not completely understood.
30206169	4	81	theme	cell	909:912	arg1	stiffness					914:922	cell stiffness	909:922	cell stiffness	909:922	We used a high-throughput technique to study a library of 5,790 loss-of-function mutants covering ~80% of the nonessential genes and correlated P. aeruginosa individual genes with cell stiffness.
30206169	9	82	with	proteins	2332:2339	arg1	roles					2346:2350	roles	2346:2350	roles	2346:2350	Using a unique high-throughput biophysical assay, we identified genes coding proteins that modulate cell stiffness in the opportunistic human pathogen Pseudomonas aeruginosa This approach enabled us to discover proteins with roles in a diverse range of biochemical pathways that influence the stiffness of P. aeruginosa cells.
30206169	3	83	theme	low	615:617	arg1	susceptibility					619:632	low susceptibility	615:632	low susceptibility to different families of antibiotics	615:669	This connection is particularly interesting in opportunistic human pathogens such as Pseudomonas aeruginosa that have a large (80%) proportion of genes of unknown function and low susceptibility to different families of antibiotics, including beta-lactams, aminoglycosides, and quinolones.
30206169	1	84	theme	bacteria	126:133	arg1	stiffness					113:121	The stiffness	109:121	The stiffness of bacteria	109:133	The stiffness of bacteria prevents cells from bursting due to the large osmotic pressure across the cell wall.
30206169	10	85	theme	cell	2640:2643	arg1	stiffness					2645:2653	cell stiffness	2640:2653	cell stiffness	2640:2653	We demonstrate that d-Ala-a component of the peptidoglycan-is tightly regulated in cells and that its accumulation reduces expression of machinery that cross-links this material and decreases cell stiffness.
30206169	3	86	theme	function	602:609	arg1	aminoglycosides					696:710	aminoglycosides	696:710	aminoglycosides	696:710	This connection is particularly interesting in opportunistic human pathogens such as Pseudomonas aeruginosa that have a large (80%) proportion of genes of unknown function and low susceptibility to different families of antibiotics, including beta-lactams, aminoglycosides, and quinolones.
30206169	3	86	theme	function	602:609	arg1	genes					585:589	genes	585:589	genes	585:589	This connection is particularly interesting in opportunistic human pathogens such as Pseudomonas aeruginosa that have a large (80%) proportion of genes of unknown function and low susceptibility to different families of antibiotics, including beta-lactams, aminoglycosides, and quinolones.
30206169	3	86	theme	function	602:609	arg1	quinolones					717:726	quinolones	717:726	quinolones	717:726	This connection is particularly interesting in opportunistic human pathogens such as Pseudomonas aeruginosa that have a large (80%) proportion of genes of unknown function and low susceptibility to different families of antibiotics, including beta-lactams, aminoglycosides, and quinolones.
30206169	3	86	theme	function	602:609	arg1	beta-lactams					682:693	beta-lactams	682:693	beta-lactams	682:693	This connection is particularly interesting in opportunistic human pathogens such as Pseudomonas aeruginosa that have a large (80%) proportion of genes of unknown function and low susceptibility to different families of antibiotics, including beta-lactams, aminoglycosides, and quinolones.
30206169	7	87	from	concentration	1366:1378	arg1	cells					1392:1396	cells	1392:1396	cells	1392:1396	An increase in the concentration of d-Ala in cells downregulated the expression of genes in peptidoglycan (PG) biosynthesis, including the peptidoglycan-cross-linking transpeptidase genes ponA and dacC Consistent with this observation, ultraperformance liquid chromatography-mass spectrometry analysis of murein from P. aeruginosa cells revealed that dadA deletion mutants contained PG with reduced cross-linking and altered composition compared to wild-type cells.IMPORTANCE The mechanical properties of bacteria are important for protecting cells against physical stress.
30206169	7	88	theme	Consistent	1549:1558	arg1	genes					1529:1533	the peptidoglycan-cross-linking transpeptidase genes	1482:1533	the peptidoglycan-cross-linking transpeptidase genes ponA and dacC Consistent with this observation	1482:1580	An increase in the concentration of d-Ala in cells downregulated the expression of genes in peptidoglycan (PG) biosynthesis, including the peptidoglycan-cross-linking transpeptidase genes ponA and dacC Consistent with this observation, ultraperformance liquid chromatography-mass spectrometry analysis of murein from P. aeruginosa cells revealed that dadA deletion mutants contained PG with reduced cross-linking and altered composition compared to wild-type cells.IMPORTANCE The mechanical properties of bacteria are important for protecting cells against physical stress.
30206169	7	88	theme	Consistent	1549:1558	arg1	ponA					1535:1538	ponA	1535:1538	ponA	1535:1538	An increase in the concentration of d-Ala in cells downregulated the expression of genes in peptidoglycan (PG) biosynthesis, including the peptidoglycan-cross-linking transpeptidase genes ponA and dacC Consistent with this observation, ultraperformance liquid chromatography-mass spectrometry analysis of murein from P. aeruginosa cells revealed that dadA deletion mutants contained PG with reduced cross-linking and altered composition compared to wild-type cells.IMPORTANCE The mechanical properties of bacteria are important for protecting cells against physical stress.
30206169	7	89	theme	reduced	1738:1744	arg1	cross-linking					1746:1758	reduced cross-linking	1738:1758	reduced cross-linking	1738:1758	An increase in the concentration of d-Ala in cells downregulated the expression of genes in peptidoglycan (PG) biosynthesis, including the peptidoglycan-cross-linking transpeptidase genes ponA and dacC Consistent with this observation, ultraperformance liquid chromatography-mass spectrometry analysis of murein from P. aeruginosa cells revealed that dadA deletion mutants contained PG with reduced cross-linking and altered composition compared to wild-type cells.IMPORTANCE The mechanical properties of bacteria are important for protecting cells against physical stress.
30206169	11	90	theme	antibacterial	2835:2847	arg1	chemotherapies					2849:2862	antibacterial chemotherapies	2835:2862	antibacterial chemotherapies	2835:2862	This research demonstrates that there is much to learn about mechanical regulation in bacteria, and these studies revealed new nonessential P. aeruginosa targets that may enhance antibacterial chemotherapies or lead to new approaches.
30206169	4	91	used	used	732:735	arg2	We					729:730	We	729:730	We	729:730	We used a high-throughput technique to study a library of 5,790 loss-of-function mutants covering ~80% of the nonessential genes and correlated P. aeruginosa individual genes with cell stiffness.
30206169	9	92	theme	P. aeruginosa	2427:2439	arg1	cells					2441:2445	P. aeruginosa cells	2427:2445	P. aeruginosa cells	2427:2445	Using a unique high-throughput biophysical assay, we identified genes coding proteins that modulate cell stiffness in the opportunistic human pathogen Pseudomonas aeruginosa This approach enabled us to discover proteins with roles in a diverse range of biochemical pathways that influence the stiffness of P. aeruginosa cells.
30206169	0	93	from	Role	38:41	arg1	Stiffness					98:106	Pseudomonas aeruginosa Cell Stiffness	70:106	Pseudomonas aeruginosa Cell Stiffness	70:106	Mechanical Genomic Studies Reveal the Role of d-Alanine Metabolism in Pseudomonas aeruginosa Cell Stiffness.
30206169	7	94	dep	contained	1720:1728	arg1	PG					1730:1731	PG	1730:1731	contained PG with reduced cross-linking and altered composition compared to wild-type cells.IMPORTANCE The mechanical properties of bacteria are important for protecting cells against physical stress	1720:1918	An increase in the concentration of d-Ala in cells downregulated the expression of genes in peptidoglycan (PG) biosynthesis, including the peptidoglycan-cross-linking transpeptidase genes ponA and dacC Consistent with this observation, ultraperformance liquid chromatography-mass spectrometry analysis of murein from P. aeruginosa cells revealed that dadA deletion mutants contained PG with reduced cross-linking and altered composition compared to wild-type cells.IMPORTANCE The mechanical properties of bacteria are important for protecting cells against physical stress.
30206169	9	95	theme	diverse	2357:2363	arg1	range					2365:2369	a diverse range	2355:2369	a diverse range of biochemical pathways that influence the stiffness of P. aeruginosa cells	2355:2445	Using a unique high-throughput biophysical assay, we identified genes coding proteins that modulate cell stiffness in the opportunistic human pathogen Pseudomonas aeruginosa This approach enabled us to discover proteins with roles in a diverse range of biochemical pathways that influence the stiffness of P. aeruginosa cells.
30206169	0	96	theme	Genomic	11:17	arg1	Studies					19:25	Mechanical Genomic Studies	0:25	Mechanical Genomic Studies	0:25	Mechanical Genomic Studies Reveal the Role of d-Alanine Metabolism in Pseudomonas aeruginosa Cell Stiffness.
30206169	7	97	theme	dadA	1698:1701	arg1	mutants					1712:1718	dadA deletion mutants	1698:1718	dadA deletion mutants	1698:1718	An increase in the concentration of d-Ala in cells downregulated the expression of genes in peptidoglycan (PG) biosynthesis, including the peptidoglycan-cross-linking transpeptidase genes ponA and dacC Consistent with this observation, ultraperformance liquid chromatography-mass spectrometry analysis of murein from P. aeruginosa cells revealed that dadA deletion mutants contained PG with reduced cross-linking and altered composition compared to wild-type cells.IMPORTANCE The mechanical properties of bacteria are important for protecting cells against physical stress.
30206169	9	98	theme	pathways	2386:2393	arg1	range					2365:2369	a diverse range	2355:2369	a diverse range of biochemical pathways that influence the stiffness of P. aeruginosa cells	2355:2445	Using a unique high-throughput biophysical assay, we identified genes coding proteins that modulate cell stiffness in the opportunistic human pathogen Pseudomonas aeruginosa This approach enabled us to discover proteins with roles in a diverse range of biochemical pathways that influence the stiffness of P. aeruginosa cells.
30206169	11	99	from	regulation	2728:2737	arg1	bacteria					2742:2749	bacteria	2742:2749	bacteria	2742:2749	This research demonstrates that there is much to learn about mechanical regulation in bacteria, and these studies revealed new nonessential P. aeruginosa targets that may enhance antibacterial chemotherapies or lead to new approaches.
30206169	7	100	theme	transpeptidase	1514:1527	arg1	genes					1529:1533	the peptidoglycan-cross-linking transpeptidase genes	1482:1533	the peptidoglycan-cross-linking transpeptidase genes ponA and dacC Consistent with this observation	1482:1580	An increase in the concentration of d-Ala in cells downregulated the expression of genes in peptidoglycan (PG) biosynthesis, including the peptidoglycan-cross-linking transpeptidase genes ponA and dacC Consistent with this observation, ultraperformance liquid chromatography-mass spectrometry analysis of murein from P. aeruginosa cells revealed that dadA deletion mutants contained PG with reduced cross-linking and altered composition compared to wild-type cells.IMPORTANCE The mechanical properties of bacteria are important for protecting cells against physical stress.
30206169	7	100	theme	transpeptidase	1514:1527	arg1	dacC					1544:1547	dacC	1544:1547	dacC	1544:1547	An increase in the concentration of d-Ala in cells downregulated the expression of genes in peptidoglycan (PG) biosynthesis, including the peptidoglycan-cross-linking transpeptidase genes ponA and dacC Consistent with this observation, ultraperformance liquid chromatography-mass spectrometry analysis of murein from P. aeruginosa cells revealed that dadA deletion mutants contained PG with reduced cross-linking and altered composition compared to wild-type cells.IMPORTANCE The mechanical properties of bacteria are important for protecting cells against physical stress.
30206169	7	100	theme	transpeptidase	1514:1527	arg1	ponA					1535:1538	ponA	1535:1538	ponA	1535:1538	An increase in the concentration of d-Ala in cells downregulated the expression of genes in peptidoglycan (PG) biosynthesis, including the peptidoglycan-cross-linking transpeptidase genes ponA and dacC Consistent with this observation, ultraperformance liquid chromatography-mass spectrometry analysis of murein from P. aeruginosa cells revealed that dadA deletion mutants contained PG with reduced cross-linking and altered composition compared to wild-type cells.IMPORTANCE The mechanical properties of bacteria are important for protecting cells against physical stress.
30206169	5	101	theme	cell	1034:1037	arg1	stiffness					1039:1047	cell stiffness	1034:1047	cell stiffness	1034:1047	We identified 42 genes coding for proteins with diverse functions that, when deleted individually, decreased cell stiffness by >20%.
30206169	7	102	theme	P. aeruginosa	1664:1676	arg1	cells					1678:1682	P. aeruginosa cells	1664:1682	P. aeruginosa cells	1664:1682	An increase in the concentration of d-Ala in cells downregulated the expression of genes in peptidoglycan (PG) biosynthesis, including the peptidoglycan-cross-linking transpeptidase genes ponA and dacC Consistent with this observation, ultraperformance liquid chromatography-mass spectrometry analysis of murein from P. aeruginosa cells revealed that dadA deletion mutants contained PG with reduced cross-linking and altered composition compared to wild-type cells.IMPORTANCE The mechanical properties of bacteria are important for protecting cells against physical stress.
30206169	11	103	theme	new	2779:2781	arg1	targets					2810:2816	new nonessential P. aeruginosa targets	2779:2816	new nonessential P. aeruginosa targets that may enhance antibacterial chemotherapies or lead to new approaches	2779:2888	This research demonstrates that there is much to learn about mechanical regulation in bacteria, and these studies revealed new nonessential P. aeruginosa targets that may enhance antibacterial chemotherapies or lead to new approaches.
30206169	9	104	theme	high-throughput	2136:2150	arg1	assay					2164:2168	a unique high-throughput biophysical assay	2127:2168	a unique high-throughput biophysical assay	2127:2168	Using a unique high-throughput biophysical assay, we identified genes coding proteins that modulate cell stiffness in the opportunistic human pathogen Pseudomonas aeruginosa This approach enabled us to discover proteins with roles in a diverse range of biochemical pathways that influence the stiffness of P. aeruginosa cells.
30206169	2	105	theme	cell	405:408	arg1	stiffness					410:418	bacterial cell stiffness	395:418	bacterial cell stiffness	395:418	Many successful antibiotic chemotherapies target elements that alter mechanical properties of bacteria, and yet a global view of the biochemistry underlying the regulation of bacterial cell stiffness is still emerging.
30206169	0	106	theme	d-Alanine	46:54	arg1	Metabolism					56:65	d-Alanine Metabolism	46:65	d-Alanine Metabolism	46:65	Mechanical Genomic Studies Reveal the Role of d-Alanine Metabolism in Pseudomonas aeruginosa Cell Stiffness.
30206169	7	107	theme	physical	1904:1911	arg1	stress					1913:1918	physical stress	1904:1918	physical stress	1904:1918	An increase in the concentration of d-Ala in cells downregulated the expression of genes in peptidoglycan (PG) biosynthesis, including the peptidoglycan-cross-linking transpeptidase genes ponA and dacC Consistent with this observation, ultraperformance liquid chromatography-mass spectrometry analysis of murein from P. aeruginosa cells revealed that dadA deletion mutants contained PG with reduced cross-linking and altered composition compared to wild-type cells.IMPORTANCE The mechanical properties of bacteria are important for protecting cells against physical stress.
30206169	6	108	theme	d-Alanine	1136:1144	arg1	DadA					1161:1164	DadA	1161:1164	DadA	1161:1164	This approach enabled us to construct a "mechanical genome" for P. aeruginosa d-Alanine dehydrogenase (DadA) is an enzyme that converts d-Ala to pyruvate that was included among the hits; when DadA was deleted, cell stiffness decreased by 18% (using multiple assays to measure mechanics).
30206169	6	108	theme	d-Alanine	1136:1144	arg1	dehydrogenase					1146:1158	P. aeruginosa d-Alanine dehydrogenase	1122:1158	P. aeruginosa d-Alanine dehydrogenase (DadA) is an enzyme that converts d-Ala to pyruvate that was included among the hits	1122:1243	This approach enabled us to construct a "mechanical genome" for P. aeruginosa d-Alanine dehydrogenase (DadA) is an enzyme that converts d-Ala to pyruvate that was included among the hits; when DadA was deleted, cell stiffness decreased by 18% (using multiple assays to measure mechanics).
30206169	3	109	from	interesting	471:481	arg1	pathogens					506:514	opportunistic human pathogens	486:514	opportunistic human pathogens such as Pseudomonas aeruginosa that have a large (80%) proportion of genes of unknown function and low susceptibility to different families of antibiotics, including beta-lactams, aminoglycosides, and quinolones	486:726	This connection is particularly interesting in opportunistic human pathogens such as Pseudomonas aeruginosa that have a large (80%) proportion of genes of unknown function and low susceptibility to different families of antibiotics, including beta-lactams, aminoglycosides, and quinolones.
30206169	3	109	from	interesting	471:481	arg1	aeruginosa					536:545	Pseudomonas aeruginosa	524:545	Pseudomonas aeruginosa that have a large (80%) proportion of genes of unknown function and low susceptibility to different families of antibiotics, including beta-lactams, aminoglycosides, and quinolones	524:726	This connection is particularly interesting in opportunistic human pathogens such as Pseudomonas aeruginosa that have a large (80%) proportion of genes of unknown function and low susceptibility to different families of antibiotics, including beta-lactams, aminoglycosides, and quinolones.
30206169	2	110	theme	global	334:339	arg1	view					341:344	a global view	332:344	a global view of the biochemistry underlying the regulation of bacterial cell stiffness	332:418	Many successful antibiotic chemotherapies target elements that alter mechanical properties of bacteria, and yet a global view of the biochemistry underlying the regulation of bacterial cell stiffness is still emerging.
30206169	3	111	theme	unknown	594:600	arg1	function					602:609	unknown function	594:609	unknown function	594:609	This connection is particularly interesting in opportunistic human pathogens such as Pseudomonas aeruginosa that have a large (80%) proportion of genes of unknown function and low susceptibility to different families of antibiotics, including beta-lactams, aminoglycosides, and quinolones.
30206169	3	112	theme	antibiotics	659:669	arg1	families					647:654	different families	637:654	different families of antibiotics	637:669	This connection is particularly interesting in opportunistic human pathogens such as Pseudomonas aeruginosa that have a large (80%) proportion of genes of unknown function and low susceptibility to different families of antibiotics, including beta-lactams, aminoglycosides, and quinolones.
30206169	3	113	theme	human	500:504	arg1	pathogens					506:514	opportunistic human pathogens	486:514	opportunistic human pathogens such as Pseudomonas aeruginosa that have a large (80%) proportion of genes of unknown function and low susceptibility to different families of antibiotics, including beta-lactams, aminoglycosides, and quinolones	486:726	This connection is particularly interesting in opportunistic human pathogens such as Pseudomonas aeruginosa that have a large (80%) proportion of genes of unknown function and low susceptibility to different families of antibiotics, including beta-lactams, aminoglycosides, and quinolones.
30206169	3	113	theme	human	500:504	arg1	aeruginosa					536:545	Pseudomonas aeruginosa	524:545	Pseudomonas aeruginosa that have a large (80%) proportion of genes of unknown function and low susceptibility to different families of antibiotics, including beta-lactams, aminoglycosides, and quinolones	524:726	This connection is particularly interesting in opportunistic human pathogens such as Pseudomonas aeruginosa that have a large (80%) proportion of genes of unknown function and low susceptibility to different families of antibiotics, including beta-lactams, aminoglycosides, and quinolones.
30206169	6	114	theme	genome	1110:1115	arg1	"					1116:1116	a "mechanical genome"	1096:1116	a "mechanical genome" for P. aeruginosa d-Alanine dehydrogenase (DadA) is an enzyme that converts d-Ala to pyruvate that was included among the hits	1096:1243	This approach enabled us to construct a "mechanical genome" for P. aeruginosa d-Alanine dehydrogenase (DadA) is an enzyme that converts d-Ala to pyruvate that was included among the hits; when DadA was deleted, cell stiffness decreased by 18% (using multiple assays to measure mechanics).
30206169	9	115	theme	genes	2185:2189	arg1	proteins					2198:2205	genes coding proteins	2185:2205	genes coding proteins that modulate cell stiffness in the opportunistic human pathogen Pseudomonas aeruginosa This approach enabled us to discover proteins with roles in a diverse range of biochemical pathways that influence the stiffness of P. aeruginosa cells	2185:2445	Using a unique high-throughput biophysical assay, we identified genes coding proteins that modulate cell stiffness in the opportunistic human pathogen Pseudomonas aeruginosa This approach enabled us to discover proteins with roles in a diverse range of biochemical pathways that influence the stiffness of P. aeruginosa cells.
30206169	4	116	theme	mutants	810:816	arg1	library					776:782	a library	774:782	a library of 5,790 loss-of-function mutants covering ~80% of the nonessential genes	774:856	We used a high-throughput technique to study a library of 5,790 loss-of-function mutants covering ~80% of the nonessential genes and correlated P. aeruginosa individual genes with cell stiffness.
30206169	2	117	theme	biochemistry	353:364	arg1	view					341:344	a global view	332:344	a global view of the biochemistry underlying the regulation of bacterial cell stiffness	332:418	Many successful antibiotic chemotherapies target elements that alter mechanical properties of bacteria, and yet a global view of the biochemistry underlying the regulation of bacterial cell stiffness is still emerging.
30102377	9	0	theme	P	1311:1311	arg1	<					1313:1313	P < 0.05	1311:1318	P < 0.05	1311:1318	Overall, the ileum had the greatest expression of farnesoid X receptor (FXR) and apical sodium-coupled bile acid transporter (ASBT) than the duodenum, jejunum, cecum, and colon (P < 0.05), whereas organic solute transporters α/β (OSTα/β) gene expression was peaked in the ileum and jejunum (P < 0.05).
30102377	9	0	theme	P	1311:1311	arg1	duodenum					1274:1281	the duodenum	1270:1281	the duodenum	1270:1281	Overall, the ileum had the greatest expression of farnesoid X receptor (FXR) and apical sodium-coupled bile acid transporter (ASBT) than the duodenum, jejunum, cecum, and colon (P < 0.05), whereas organic solute transporters α/β (OSTα/β) gene expression was peaked in the ileum and jejunum (P < 0.05).
30102377	7	1	theme	P	1017:1017	arg1	colon					1010:1014	the colon	1006:1014	the colon (P < 0.05)	1006:1025	The ratio of primary, secondary, taurine-conjugated, and glycine-conjugated BAs in the ileal pool was about 46:15:9:30, whereas it was 28:61:1:11 in the cecum and 22:65:3:9 in the colon (P < 0.05).
30102377	7	1	theme	P	1017:1017	arg1	<					1019:1019	P < 0.05	1017:1024	P < 0.05	1017:1024	The ratio of primary, secondary, taurine-conjugated, and glycine-conjugated BAs in the ileal pool was about 46:15:9:30, whereas it was 28:61:1:11 in the cecum and 22:65:3:9 in the colon (P < 0.05).
30102377	15	2	theme	greater	2180:2186	arg1	size					2196:2199	the greater BA pool size	2176:2199	the greater BA pool size	2176:2199	In conclusion, the abundant distribution of BA transporters and the greater BA pool size suggests the ileum as the major site for intestinal BA reabsorption in pigs.
30102377	3	3	theme	%	420:420	arg1	pectin					422:427	5% pectin	419:427	5% pectin	419:427	Twelve young pigs (11.05 ± 0.11 kg) were randomly divided into 2 groups and fed corn-soybean meal diets with either 5% pectin or cornstarch for 72 d.
30102377	11	4	theme	P	1684:1684	arg1	<					1686:1686	P < 0.05	1684:1691	P < 0.05	1684:1691	Greater expression of G protein-coupled bile acid receptor and multidrug resistance protein 3 (MRP3) was found in the cecum and colon (P < 0.05).
30102377	11	4	theme	P	1684:1684	arg1	cecum					1667:1671	cecum	1667:1671	cecum	1667:1671	Greater expression of G protein-coupled bile acid receptor and multidrug resistance protein 3 (MRP3) was found in the cecum and colon (P < 0.05).
30102377	4	5	theme	total	478:482	arg1	cholesterol					484:494	total cholesterol	478:494	total cholesterol	478:494	In pigs fed with pectin, total cholesterol and low-density lipoprotein cholesterol (LDL-C) were lowered but high-density lipoprotein (HDL-C) was increased (P < 0.05).
30102377	7	6	theme	ileal	917:921	arg1	pool					923:926	the ileal pool	913:926	the ileal pool	913:926	The ratio of primary, secondary, taurine-conjugated, and glycine-conjugated BAs in the ileal pool was about 46:15:9:30, whereas it was 28:61:1:11 in the cecum and 22:65:3:9 in the colon (P < 0.05).
30102377	2	7	theme	intestinal	240:249	arg1	transport					254:262	intestinal BA transport	240:262	intestinal BA transport	240:262	The current study was aimed to investigate intestinal BA transport at the molecular level in a pig model.
30102377	16	8	theme	BA	2414:2415	arg1	transport					2417:2425	the overall BA transport	2402:2425	the overall BA transport in the cecum	2402:2438	In the ileum, pectin increased in-and-out BA transport on the apical membrane by increasing ASBT and MRP2, but it increased the overall BA transport in the cecum by increasing OSTα/β and MRP3.
30102377	5	9	theme	bile	741:744	arg1	acid					746:749	total bile acid	735:749	serum total bile acid	729:749	Serum triglycerides tended to be lower in the pectin-fed animals (P = 0.093), whereas no change was noted in serum total bile acid.
30102377	15	10	theme	BA	2253:2254	arg1	reabsorption					2256:2267	intestinal BA reabsorption	2242:2267	intestinal BA reabsorption in pigs	2242:2275	In conclusion, the abundant distribution of BA transporters and the greater BA pool size suggests the ileum as the major site for intestinal BA reabsorption in pigs.
30102377	10	11	theme	P	1538:1538	arg1	<					1540:1540	P < 0.05	1538:1545	P < 0.05	1538:1545	Expression multidrug resistance protein 2 (MRP2) gradually decreased towards the end of the intestine (P < 0.05).
30102377	3	12	with	diets	401:405	arg1	cornstarch					432:441	cornstarch	432:441	cornstarch	432:441	Twelve young pigs (11.05 ± 0.11 kg) were randomly divided into 2 groups and fed corn-soybean meal diets with either 5% pectin or cornstarch for 72 d.
30102377	3	12	with	diets	401:405	arg1	pectin					422:427	5% pectin	419:427	5% pectin	419:427	Twelve young pigs (11.05 ± 0.11 kg) were randomly divided into 2 groups and fed corn-soybean meal diets with either 5% pectin or cornstarch for 72 d.
30102377	11	13	theme	receptor	1599:1606	arg1	expression					1557:1566	Greater expression	1549:1566	Greater expression of G protein-coupled bile acid receptor and multidrug resistance protein 3 (MRP3)	1549:1648	Greater expression of G protein-coupled bile acid receptor and multidrug resistance protein 3 (MRP3) was found in the cecum and colon (P < 0.05).
30102377	9	14	theme	organic	1330:1336	arg1	transporters					1345:1356	organic solute transporters	1330:1356	organic solute transporters α/β (OSTα/β) gene expression	1330:1385	Overall, the ileum had the greatest expression of farnesoid X receptor (FXR) and apical sodium-coupled bile acid transporter (ASBT) than the duodenum, jejunum, cecum, and colon (P < 0.05), whereas organic solute transporters α/β (OSTα/β) gene expression was peaked in the ileum and jejunum (P < 0.05).
30102377	4	15	theme	high-density	561:572	arg1	HDL-C					587:591	HDL-C	587:591	HDL-C	587:591	In pigs fed with pectin, total cholesterol and low-density lipoprotein cholesterol (LDL-C) were lowered but high-density lipoprotein (HDL-C) was increased (P < 0.05).
30102377	4	15	theme	high-density	561:572	arg1	lipoprotein					574:584	high-density lipoprotein	561:584	high-density lipoprotein (HDL-C)	561:592	In pigs fed with pectin, total cholesterol and low-density lipoprotein cholesterol (LDL-C) were lowered but high-density lipoprotein (HDL-C) was increased (P < 0.05).
30102377	7	16	theme	glycine-conjugated	887:904	arg1	BAs					906:908	primary, secondary, taurine-conjugated, and glycine-conjugated BAs	843:908	primary, secondary, taurine-conjugated, and glycine-conjugated BAs in the ileal pool	843:926	The ratio of primary, secondary, taurine-conjugated, and glycine-conjugated BAs in the ileal pool was about 46:15:9:30, whereas it was 28:61:1:11 in the cecum and 22:65:3:9 in the colon (P < 0.05).
30102377	9	17	theme	greatest	1160:1167	arg1	expression					1169:1178	the greatest expression	1156:1178	the greatest expression of farnesoid X receptor (FXR) and apical sodium-coupled bile acid transporter (ASBT)	1156:1263	Overall, the ileum had the greatest expression of farnesoid X receptor (FXR) and apical sodium-coupled bile acid transporter (ASBT) than the duodenum, jejunum, cecum, and colon (P < 0.05), whereas organic solute transporters α/β (OSTα/β) gene expression was peaked in the ileum and jejunum (P < 0.05).
30102377	9	18	theme	transporters	1345:1356	arg1	expression					1376:1385	organic solute transporters α/β (OSTα/β) gene expression	1330:1385	organic solute transporters α/β (OSTα/β) gene expression	1330:1385	Overall, the ileum had the greatest expression of farnesoid X receptor (FXR) and apical sodium-coupled bile acid transporter (ASBT) than the duodenum, jejunum, cecum, and colon (P < 0.05), whereas organic solute transporters α/β (OSTα/β) gene expression was peaked in the ileum and jejunum (P < 0.05).
30102377	15	19	theme	major	2227:2231	arg1	ileum					2214:2218	the ileum	2210:2218	the ileum	2210:2218	In conclusion, the abundant distribution of BA transporters and the greater BA pool size suggests the ileum as the major site for intestinal BA reabsorption in pigs.
30102377	15	19	theme	major	2227:2231	arg1	site					2233:2236	the major site	2223:2236	the major site for intestinal BA reabsorption in pigs	2223:2275	In conclusion, the abundant distribution of BA transporters and the greater BA pool size suggests the ileum as the major site for intestinal BA reabsorption in pigs.
30102377	13	20	theme	PEC-fed	1914:1920	arg1	pigs					1922:1925	PEC-fed pigs	1914:1925	PEC-fed pigs (P < 0.05)	1914:1936	But FXR, ASBT, and MRP2 were upregulated in the ileum and FXR, OSTα/β, MRP2, and MRP3 in the cecum of PEC-fed pigs (P < 0.05).
30102377	13	20	theme	PEC-fed	1914:1920	arg1	<					1930:1930	P < 0.05	1928:1935	P < 0.05	1928:1935	But FXR, ASBT, and MRP2 were upregulated in the ileum and FXR, OSTα/β, MRP2, and MRP3 in the cecum of PEC-fed pigs (P < 0.05).
30102377	11	21	theme	protein	1633:1639	arg1	expression					1557:1566	Greater expression	1549:1566	Greater expression of G protein-coupled bile acid receptor and multidrug resistance protein 3 (MRP3)	1549:1648	Greater expression of G protein-coupled bile acid receptor and multidrug resistance protein 3 (MRP3) was found in the cecum and colon (P < 0.05).
30102377	1	22	theme	acid	175:178	arg1	metabolism					185:194	bile acid (BA) metabolism	170:194	bile acid (BA) metabolism	170:194	Pectin has been known to lower circulating cholesterol by interacting with bile acid (BA) metabolism.
30102377	6	23	theme	intestine	776:784	arg1	length					762:767	the length	758:767	the length of the intestine	758:784	Along the length of the intestine, the size and composition of BA pools vary.
30102377	7	24	from	cecum	983:987	arg1	colon					1010:1014	the colon	1006:1014	the colon (P < 0.05)	1006:1025	The ratio of primary, secondary, taurine-conjugated, and glycine-conjugated BAs in the ileal pool was about 46:15:9:30, whereas it was 28:61:1:11 in the cecum and 22:65:3:9 in the colon (P < 0.05).
30102377	7	24	from	cecum	983:987	arg1	<					1019:1019	P < 0.05	1017:1024	P < 0.05	1017:1024	The ratio of primary, secondary, taurine-conjugated, and glycine-conjugated BAs in the ileal pool was about 46:15:9:30, whereas it was 28:61:1:11 in the cecum and 22:65:3:9 in the colon (P < 0.05).
30102377	9	25	theme	X	1193:1193	arg1	FXR					1205:1207	FXR	1205:1207	FXR	1205:1207	Overall, the ileum had the greatest expression of farnesoid X receptor (FXR) and apical sodium-coupled bile acid transporter (ASBT) than the duodenum, jejunum, cecum, and colon (P < 0.05), whereas organic solute transporters α/β (OSTα/β) gene expression was peaked in the ileum and jejunum (P < 0.05).
30102377	9	25	theme	X	1193:1193	arg1	receptor					1195:1202	farnesoid X receptor	1183:1202	farnesoid X receptor (FXR)	1183:1208	Overall, the ileum had the greatest expression of farnesoid X receptor (FXR) and apical sodium-coupled bile acid transporter (ASBT) than the duodenum, jejunum, cecum, and colon (P < 0.05), whereas organic solute transporters α/β (OSTα/β) gene expression was peaked in the ileum and jejunum (P < 0.05).
30102377	7	26	from	22:65:3:9	993:1001	arg1	colon					1010:1014	the colon	1006:1014	the colon (P < 0.05)	1006:1025	The ratio of primary, secondary, taurine-conjugated, and glycine-conjugated BAs in the ileal pool was about 46:15:9:30, whereas it was 28:61:1:11 in the cecum and 22:65:3:9 in the colon (P < 0.05).
30102377	7	26	from	22:65:3:9	993:1001	arg1	<					1019:1019	P < 0.05	1017:1024	P < 0.05	1017:1024	The ratio of primary, secondary, taurine-conjugated, and glycine-conjugated BAs in the ileal pool was about 46:15:9:30, whereas it was 28:61:1:11 in the cecum and 22:65:3:9 in the colon (P < 0.05).
30102377	8	27	theme	lithocholic	1042:1052	arg1	acid					1054:1057	lithocholic acid	1042:1057	lithocholic acid	1042:1057	In the feces, lithocholic acid and ursodeoxycholic acid (UDCA) made up of over 97% of the total BA pool.
30102377	14	28	theme	bile	1999:2002	arg1	synthase					2013:2020	bile acid-CoA synthase	1999:2020	bile acid-CoA synthase	1999:2020	Liver enzymes involved in BA biosynthesis (CYP7A1, CYP27A1, bile acid-CoA synthase, and bile acid-CoA:amino acid N acyltransferase) were not affected by pectin consumption.
30102377	4	29	theme	P	609:609	arg1	<					611:611	P < 0.05	609:616	P < 0.05	609:616	In pigs fed with pectin, total cholesterol and low-density lipoprotein cholesterol (LDL-C) were lowered but high-density lipoprotein (HDL-C) was increased (P < 0.05).
30102377	15	30	theme	pool	2191:2194	arg1	size					2196:2199	the greater BA pool size	2176:2199	the greater BA pool size	2176:2199	In conclusion, the abundant distribution of BA transporters and the greater BA pool size suggests the ileum as the major site for intestinal BA reabsorption in pigs.
30102377	10	31	theme	resistance	1456:1465	arg1	MRP2					1478:1481	MRP2	1478:1481	MRP2	1478:1481	Expression multidrug resistance protein 2 (MRP2) gradually decreased towards the end of the intestine (P < 0.05).
30102377	10	31	theme	resistance	1456:1465	arg1	protein					1467:1473	multidrug resistance protein 2	1446:1475	Expression multidrug resistance protein 2 (MRP2)	1435:1482	Expression multidrug resistance protein 2 (MRP2) gradually decreased towards the end of the intestine (P < 0.05).
30102377	5	32	dep	lower	653:657	arg1	=					688:688	=	688:688	=	688:688	Serum triglycerides tended to be lower in the pectin-fed animals (P = 0.093), whereas no change was noted in serum total bile acid.
30102377	2	33	theme	current	201:207	arg1	study					209:213	The current study	197:213	The current study	197:213	The current study was aimed to investigate intestinal BA transport at the molecular level in a pig model.
30102377	9	34	theme	apical	1214:1219	arg1	ASBT					1259:1262	ASBT	1259:1262	ASBT	1259:1262	Overall, the ileum had the greatest expression of farnesoid X receptor (FXR) and apical sodium-coupled bile acid transporter (ASBT) than the duodenum, jejunum, cecum, and colon (P < 0.05), whereas organic solute transporters α/β (OSTα/β) gene expression was peaked in the ileum and jejunum (P < 0.05).
30102377	9	34	theme	apical	1214:1219	arg1	transporter					1246:1256	apical sodium-coupled bile acid transporter	1214:1256	apical sodium-coupled bile acid transporter (ASBT)	1214:1263	Overall, the ileum had the greatest expression of farnesoid X receptor (FXR) and apical sodium-coupled bile acid transporter (ASBT) than the duodenum, jejunum, cecum, and colon (P < 0.05), whereas organic solute transporters α/β (OSTα/β) gene expression was peaked in the ileum and jejunum (P < 0.05).
30102377	6	35	theme	pools	818:822	arg1	composition					800:810	composition	800:810	composition	800:810	Along the length of the intestine, the size and composition of BA pools vary.
30102377	6	35	theme	pools	818:822	arg1	size					791:794	size	791:794	size	791:794	Along the length of the intestine, the size and composition of BA pools vary.
30102377	12	36	theme	=	1773:1773	arg1	P					1771:1771	P = 0.088	1771:1779	P = 0.088	1771:1779	In pigs fed with 5% pectin, only cecal UDCA (P = 0.097) and hyocholic acid (P = 0.088) showed a decreasing tendency.
30102377	12	36	theme	=	1773:1773	arg1	acid					1765:1768	hyocholic acid	1755:1768	hyocholic acid (P = 0.088)	1755:1780	In pigs fed with 5% pectin, only cecal UDCA (P = 0.097) and hyocholic acid (P = 0.088) showed a decreasing tendency.
30102377	0	37	theme	intestinal	58:67	arg1	acids					74:78	intestinal bile acids	58:78	intestinal bile acids	58:78	Effects of dietary pectin on the profile and transport of intestinal bile acids in young pigs.
30102377	1	38	theme	circulating	126:136	arg1	cholesterol					138:148	circulating cholesterol	126:148	circulating cholesterol	126:148	Pectin has been known to lower circulating cholesterol by interacting with bile acid (BA) metabolism.
30102377	0	39	from	Effects	0:6	arg1	transport					45:53	transport	45:53	transport	45:53	Effects of dietary pectin on the profile and transport of intestinal bile acids in young pigs.
30102377	0	39	from	Effects	0:6	arg1	profile					33:39	profile	33:39	profile	33:39	Effects of dietary pectin on the profile and transport of intestinal bile acids in young pigs.
30102377	9	40	contain	had	1152:1154	arg1	ileum					1146:1150	the ileum	1142:1150	the ileum	1142:1150	Overall, the ileum had the greatest expression of farnesoid X receptor (FXR) and apical sodium-coupled bile acid transporter (ASBT) than the duodenum, jejunum, cecum, and colon (P < 0.05), whereas organic solute transporters α/β (OSTα/β) gene expression was peaked in the ileum and jejunum (P < 0.05).
30102377	9	40	contain	had	1152:1154	arg2	expression					1169:1178	the greatest expression	1156:1178	the greatest expression of farnesoid X receptor (FXR) and apical sodium-coupled bile acid transporter (ASBT)	1156:1263	Overall, the ileum had the greatest expression of farnesoid X receptor (FXR) and apical sodium-coupled bile acid transporter (ASBT) than the duodenum, jejunum, cecum, and colon (P < 0.05), whereas organic solute transporters α/β (OSTα/β) gene expression was peaked in the ileum and jejunum (P < 0.05).
30102377	0	41	theme	acids	74:78	arg1	transport					45:53	transport	45:53	transport	45:53	Effects of dietary pectin on the profile and transport of intestinal bile acids in young pigs.
30102377	0	41	theme	acids	74:78	arg1	profile					33:39	profile	33:39	profile	33:39	Effects of dietary pectin on the profile and transport of intestinal bile acids in young pigs.
30102377	11	42	theme	multidrug	1612:1620	arg1	protein					1633:1639	multidrug resistance protein 3	1612:1641	multidrug resistance protein 3 (MRP3)	1612:1648	Greater expression of G protein-coupled bile acid receptor and multidrug resistance protein 3 (MRP3) was found in the cecum and colon (P < 0.05).
30102377	11	42	theme	multidrug	1612:1620	arg1	MRP3					1644:1647	MRP3	1644:1647	MRP3	1644:1647	Greater expression of G protein-coupled bile acid receptor and multidrug resistance protein 3 (MRP3) was found in the cecum and colon (P < 0.05).
30102377	9	43	theme	transporter	1246:1256	arg1	expression					1169:1178	the greatest expression	1156:1178	the greatest expression of farnesoid X receptor (FXR) and apical sodium-coupled bile acid transporter (ASBT)	1156:1263	Overall, the ileum had the greatest expression of farnesoid X receptor (FXR) and apical sodium-coupled bile acid transporter (ASBT) than the duodenum, jejunum, cecum, and colon (P < 0.05), whereas organic solute transporters α/β (OSTα/β) gene expression was peaked in the ileum and jejunum (P < 0.05).
30102377	0	44	from	profile	33:39	arg1	pigs					89:92	young pigs	83:92	young pigs	83:92	Effects of dietary pectin on the profile and transport of intestinal bile acids in young pigs.
30102377	0	45	theme	young	83:87	arg1	pigs					89:92	young pigs	83:92	young pigs	83:92	Effects of dietary pectin on the profile and transport of intestinal bile acids in young pigs.
30102377	3	46	theme	11.05	322:326	arg1	±					328:328	±	328:328	±	328:328	Twelve young pigs (11.05 ± 0.11 kg) were randomly divided into 2 groups and fed corn-soybean meal diets with either 5% pectin or cornstarch for 72 d.
30102377	5	47	dep	serum	729:733	arg1	acid					746:749	total bile acid	735:749	serum total bile acid	729:749	Serum triglycerides tended to be lower in the pectin-fed animals (P = 0.093), whereas no change was noted in serum total bile acid.
30102377	16	48	theme	BA	2320:2321	arg1	transport					2323:2331	in-and-out BA transport	2309:2331	in-and-out BA transport on the apical membrane	2309:2354	In the ileum, pectin increased in-and-out BA transport on the apical membrane by increasing ASBT and MRP2, but it increased the overall BA transport in the cecum by increasing OSTα/β and MRP3.
30102377	13	49	theme	P	1928:1928	arg1	pigs					1922:1925	PEC-fed pigs	1914:1925	PEC-fed pigs (P < 0.05)	1914:1936	But FXR, ASBT, and MRP2 were upregulated in the ileum and FXR, OSTα/β, MRP2, and MRP3 in the cecum of PEC-fed pigs (P < 0.05).
30102377	13	49	theme	P	1928:1928	arg1	<					1930:1930	P < 0.05	1928:1935	P < 0.05	1928:1935	But FXR, ASBT, and MRP2 were upregulated in the ileum and FXR, OSTα/β, MRP2, and MRP3 in the cecum of PEC-fed pigs (P < 0.05).
30102377	0	50	theme	dietary	11:17	arg1	pectin					19:24	dietary pectin	11:24	dietary pectin	11:24	Effects of dietary pectin on the profile and transport of intestinal bile acids in young pigs.
30102377	8	51	theme	total	1118:1122	arg1	pool					1127:1130	the total BA pool	1114:1130	the total BA pool	1114:1130	In the feces, lithocholic acid and ursodeoxycholic acid (UDCA) made up of over 97% of the total BA pool.
30102377	16	52	theme	apical	2340:2345	arg1	membrane					2347:2354	the apical membrane	2336:2354	the apical membrane	2336:2354	In the ileum, pectin increased in-and-out BA transport on the apical membrane by increasing ASBT and MRP2, but it increased the overall BA transport in the cecum by increasing OSTα/β and MRP3.
30102377	14	53	theme	Liver	1939:1943	arg1	enzymes					1945:1951	Liver enzymes	1939:1951	Liver enzymes involved in BA biosynthesis (CYP7A1, CYP27A1, bile acid-CoA synthase, and bile acid-CoA:amino acid N acyltransferase)	1939:2069	Liver enzymes involved in BA biosynthesis (CYP7A1, CYP27A1, bile acid-CoA synthase, and bile acid-CoA:amino acid N acyltransferase) were not affected by pectin consumption.
30102377	0	54	dep	profile	33:39	arg1	the					29:31	the	29:31	the	29:31	Effects of dietary pectin on the profile and transport of intestinal bile acids in young pigs.
30102377	3	55	theme	0.11	330:333	arg1	±					328:328	±	328:328	±	328:328	Twelve young pigs (11.05 ± 0.11 kg) were randomly divided into 2 groups and fed corn-soybean meal diets with either 5% pectin or cornstarch for 72 d.
30102377	8	56	theme	pool	1127:1130	arg1	pool					1127:1130	the total BA pool	1114:1130	the total BA pool	1114:1130	In the feces, lithocholic acid and ursodeoxycholic acid (UDCA) made up of over 97% of the total BA pool.
30102377	8	56	theme	pool	1127:1130	arg1	%					1109:1109	up of over 97%	1096:1109	up of over 97% of the total BA pool	1096:1130	In the feces, lithocholic acid and ursodeoxycholic acid (UDCA) made up of over 97% of the total BA pool.
30102377	2	57	theme	pig	292:294	arg1	model					296:300	a pig model	290:300	a pig model	290:300	The current study was aimed to investigate intestinal BA transport at the molecular level in a pig model.
30102377	15	58	theme	BA	2156:2157	arg1	transporters					2159:2170	BA transporters	2156:2170	BA transporters	2156:2170	In conclusion, the abundant distribution of BA transporters and the greater BA pool size suggests the ileum as the major site for intestinal BA reabsorption in pigs.
30102377	3	59	theme	meal	396:399	arg1	diets					401:405	corn-soybean meal diets	383:405	corn-soybean meal diets with either 5% pectin or cornstarch for 72 d	383:450	Twelve young pigs (11.05 ± 0.11 kg) were randomly divided into 2 groups and fed corn-soybean meal diets with either 5% pectin or cornstarch for 72 d.
30102377	8	60	theme	ursodeoxycholic	1063:1077	arg1	acid					1079:1082	ursodeoxycholic acid	1063:1082	ursodeoxycholic acid (UDCA)	1063:1089	In the feces, lithocholic acid and ursodeoxycholic acid (UDCA) made up of over 97% of the total BA pool.
30102377	8	60	theme	ursodeoxycholic	1063:1077	arg1	UDCA					1085:1088	UDCA	1085:1088	UDCA	1085:1088	In the feces, lithocholic acid and ursodeoxycholic acid (UDCA) made up of over 97% of the total BA pool.
30102377	12	61	theme	5	1712:1712	arg1	%					1713:1713	%	1713:1713	%	1713:1713	In pigs fed with 5% pectin, only cecal UDCA (P = 0.097) and hyocholic acid (P = 0.088) showed a decreasing tendency.
30102377	14	62	theme	N	2052:2052	arg1	acyltransferase					2054:2068	amino acid N acyltransferase	2041:2068	CYP7A1, CYP27A1, bile acid-CoA synthase, and bile acid-CoA:amino acid N acyltransferase	1982:2068	Liver enzymes involved in BA biosynthesis (CYP7A1, CYP27A1, bile acid-CoA synthase, and bile acid-CoA:amino acid N acyltransferase) were not affected by pectin consumption.
30102377	4	63	theme	low-density	500:510	arg1	LDL-C					537:541	LDL-C	537:541	LDL-C	537:541	In pigs fed with pectin, total cholesterol and low-density lipoprotein cholesterol (LDL-C) were lowered but high-density lipoprotein (HDL-C) was increased (P < 0.05).
30102377	4	63	theme	low-density	500:510	arg1	cholesterol					524:534	low-density lipoprotein cholesterol	500:534	low-density lipoprotein cholesterol (LDL-C)	500:542	In pigs fed with pectin, total cholesterol and low-density lipoprotein cholesterol (LDL-C) were lowered but high-density lipoprotein (HDL-C) was increased (P < 0.05).
30102377	3	64	theme	5	419:419	arg1	%					420:420	%	420:420	%	420:420	Twelve young pigs (11.05 ± 0.11 kg) were randomly divided into 2 groups and fed corn-soybean meal diets with either 5% pectin or cornstarch for 72 d.
30102377	16	65	from	transport	2323:2331	arg1	membrane					2347:2354	the apical membrane	2336:2354	the apical membrane	2336:2354	In the ileum, pectin increased in-and-out BA transport on the apical membrane by increasing ASBT and MRP2, but it increased the overall BA transport in the cecum by increasing OSTα/β and MRP3.
30102377	16	66	theme	in-and-out	2309:2318	arg1	transport					2323:2331	in-and-out BA transport	2309:2331	in-and-out BA transport on the apical membrane	2309:2354	In the ileum, pectin increased in-and-out BA transport on the apical membrane by increasing ASBT and MRP2, but it increased the overall BA transport in the cecum by increasing OSTα/β and MRP3.
30102377	14	67	theme	amino	2041:2045	arg1	acyltransferase					2054:2068	amino acid N acyltransferase	2041:2068	CYP7A1, CYP27A1, bile acid-CoA synthase, and bile acid-CoA:amino acid N acyltransferase	1982:2068	Liver enzymes involved in BA biosynthesis (CYP7A1, CYP27A1, bile acid-CoA synthase, and bile acid-CoA:amino acid N acyltransferase) were not affected by pectin consumption.
30102377	14	68	dep	CYP7A1	1982:1987	arg1	acyltransferase					2054:2068	amino acid N acyltransferase	2041:2068	CYP7A1, CYP27A1, bile acid-CoA synthase, and bile acid-CoA:amino acid N acyltransferase	1982:2068	Liver enzymes involved in BA biosynthesis (CYP7A1, CYP27A1, bile acid-CoA synthase, and bile acid-CoA:amino acid N acyltransferase) were not affected by pectin consumption.
30102377	15	69	theme	transporters	2159:2170	arg1	size					2196:2199	the greater BA pool size	2176:2199	the greater BA pool size	2176:2199	In conclusion, the abundant distribution of BA transporters and the greater BA pool size suggests the ileum as the major site for intestinal BA reabsorption in pigs.
30102377	15	69	theme	transporters	2159:2170	arg1	distribution					2140:2151	the abundant distribution	2127:2151	the abundant distribution of BA transporters	2127:2170	In conclusion, the abundant distribution of BA transporters and the greater BA pool size suggests the ileum as the major site for intestinal BA reabsorption in pigs.
30102377	1	70	dep	cholesterol	138:148	arg1	lower					120:124	lower	120:124	lower	120:124	Pectin has been known to lower circulating cholesterol by interacting with bile acid (BA) metabolism.
30102377	2	71	theme	BA	251:252	arg1	transport					254:262	intestinal BA transport	240:262	intestinal BA transport	240:262	The current study was aimed to investigate intestinal BA transport at the molecular level in a pig model.
30102377	11	72	dep	cecum	1667:1671	arg1	the					1663:1665	the	1663:1665	the	1663:1665	Greater expression of G protein-coupled bile acid receptor and multidrug resistance protein 3 (MRP3) was found in the cecum and colon (P < 0.05).
30102377	7	73	theme	BAs	906:908	arg1	ratio					834:838	The ratio	830:838	The ratio of primary, secondary, taurine-conjugated, and glycine-conjugated BAs in the ileal pool	830:926	The ratio of primary, secondary, taurine-conjugated, and glycine-conjugated BAs in the ileal pool was about 46:15:9:30, whereas it was 28:61:1:11 in the cecum and 22:65:3:9 in the colon (P < 0.05).
30102377	7	73	theme	BAs	906:908	arg1	46:15:9:30					938:947	46:15:9:30	938:947	46:15:9:30	938:947	The ratio of primary, secondary, taurine-conjugated, and glycine-conjugated BAs in the ileal pool was about 46:15:9:30, whereas it was 28:61:1:11 in the cecum and 22:65:3:9 in the colon (P < 0.05).
30102377	11	74	theme	acid	1594:1597	arg1	receptor					1599:1606	G protein-coupled bile acid receptor	1571:1606	G protein-coupled bile acid receptor	1571:1606	Greater expression of G protein-coupled bile acid receptor and multidrug resistance protein 3 (MRP3) was found in the cecum and colon (P < 0.05).
30102377	16	75	theme	overall	2406:2412	arg1	transport					2417:2425	the overall BA transport	2402:2425	the overall BA transport in the cecum	2402:2438	In the ileum, pectin increased in-and-out BA transport on the apical membrane by increasing ASBT and MRP2, but it increased the overall BA transport in the cecum by increasing OSTα/β and MRP3.
30102377	10	76	dep	Expression	1435:1444	arg1	MRP2					1478:1481	MRP2	1478:1481	MRP2	1478:1481	Expression multidrug resistance protein 2 (MRP2) gradually decreased towards the end of the intestine (P < 0.05).
30102377	10	76	dep	Expression	1435:1444	arg1	protein					1467:1473	multidrug resistance protein 2	1446:1475	Expression multidrug resistance protein 2 (MRP2)	1435:1482	Expression multidrug resistance protein 2 (MRP2) gradually decreased towards the end of the intestine (P < 0.05).
30102377	0	77	from	transport	45:53	arg1	pigs					89:92	young pigs	83:92	young pigs	83:92	Effects of dietary pectin on the profile and transport of intestinal bile acids in young pigs.
30102377	15	78	theme	abundant	2131:2138	arg1	distribution					2140:2151	the abundant distribution	2127:2151	the abundant distribution of BA transporters	2127:2170	In conclusion, the abundant distribution of BA transporters and the greater BA pool size suggests the ileum as the major site for intestinal BA reabsorption in pigs.
30102377	2	79	theme	molecular	271:279	arg1	level					281:285	the molecular level	267:285	the molecular level	267:285	The current study was aimed to investigate intestinal BA transport at the molecular level in a pig model.
30102377	9	80	theme	solute	1338:1343	arg1	transporters					1345:1356	organic solute transporters	1330:1356	organic solute transporters α/β (OSTα/β) gene expression	1330:1385	Overall, the ileum had the greatest expression of farnesoid X receptor (FXR) and apical sodium-coupled bile acid transporter (ASBT) than the duodenum, jejunum, cecum, and colon (P < 0.05), whereas organic solute transporters α/β (OSTα/β) gene expression was peaked in the ileum and jejunum (P < 0.05).
30102377	15	81	theme	intestinal	2242:2251	arg1	reabsorption					2256:2267	intestinal BA reabsorption	2242:2267	intestinal BA reabsorption in pigs	2242:2275	In conclusion, the abundant distribution of BA transporters and the greater BA pool size suggests the ileum as the major site for intestinal BA reabsorption in pigs.
30102377	7	82	theme	taurine-conjugated	863:880	arg1	BAs					906:908	primary, secondary, taurine-conjugated, and glycine-conjugated BAs	843:908	primary, secondary, taurine-conjugated, and glycine-conjugated BAs in the ileal pool	843:926	The ratio of primary, secondary, taurine-conjugated, and glycine-conjugated BAs in the ileal pool was about 46:15:9:30, whereas it was 28:61:1:11 in the cecum and 22:65:3:9 in the colon (P < 0.05).
30102377	11	83	theme	resistance	1622:1631	arg1	protein					1633:1639	multidrug resistance protein 3	1612:1641	multidrug resistance protein 3 (MRP3)	1612:1648	Greater expression of G protein-coupled bile acid receptor and multidrug resistance protein 3 (MRP3) was found in the cecum and colon (P < 0.05).
30102377	11	83	theme	resistance	1622:1631	arg1	MRP3					1644:1647	MRP3	1644:1647	MRP3	1644:1647	Greater expression of G protein-coupled bile acid receptor and multidrug resistance protein 3 (MRP3) was found in the cecum and colon (P < 0.05).
30102377	1	84	theme	bile	170:173	arg1	acid					175:178	bile acid	170:178	bile acid (BA) metabolism	170:194	Pectin has been known to lower circulating cholesterol by interacting with bile acid (BA) metabolism.
30102377	1	84	theme	bile	170:173	arg1	BA					181:182	BA	181:182	BA	181:182	Pectin has been known to lower circulating cholesterol by interacting with bile acid (BA) metabolism.
30102377	14	85	theme	bile	2027:2030	arg1	acid-CoA					2032:2039	bile acid-CoA	2027:2039	bile acid-CoA	2027:2039	Liver enzymes involved in BA biosynthesis (CYP7A1, CYP27A1, bile acid-CoA synthase, and bile acid-CoA:amino acid N acyltransferase) were not affected by pectin consumption.
30102377	9	86	theme	α/β	1358:1360	arg1	expression					1376:1385	organic solute transporters α/β (OSTα/β) gene expression	1330:1385	organic solute transporters α/β (OSTα/β) gene expression	1330:1385	Overall, the ileum had the greatest expression of farnesoid X receptor (FXR) and apical sodium-coupled bile acid transporter (ASBT) than the duodenum, jejunum, cecum, and colon (P < 0.05), whereas organic solute transporters α/β (OSTα/β) gene expression was peaked in the ileum and jejunum (P < 0.05).
30102377	5	87	theme	total	735:739	arg1	acid					746:749	total bile acid	735:749	serum total bile acid	729:749	Serum triglycerides tended to be lower in the pectin-fed animals (P = 0.093), whereas no change was noted in serum total bile acid.
30102377	9	88	theme	bile	1236:1239	arg1	ASBT					1259:1262	ASBT	1259:1262	ASBT	1259:1262	Overall, the ileum had the greatest expression of farnesoid X receptor (FXR) and apical sodium-coupled bile acid transporter (ASBT) than the duodenum, jejunum, cecum, and colon (P < 0.05), whereas organic solute transporters α/β (OSTα/β) gene expression was peaked in the ileum and jejunum (P < 0.05).
30102377	9	88	theme	bile	1236:1239	arg1	transporter					1246:1256	apical sodium-coupled bile acid transporter	1214:1256	apical sodium-coupled bile acid transporter (ASBT)	1214:1263	Overall, the ileum had the greatest expression of farnesoid X receptor (FXR) and apical sodium-coupled bile acid transporter (ASBT) than the duodenum, jejunum, cecum, and colon (P < 0.05), whereas organic solute transporters α/β (OSTα/β) gene expression was peaked in the ileum and jejunum (P < 0.05).
30102377	7	89	theme	secondary	852:860	arg1	BAs					906:908	primary, secondary, taurine-conjugated, and glycine-conjugated BAs	843:908	primary, secondary, taurine-conjugated, and glycine-conjugated BAs in the ileal pool	843:926	The ratio of primary, secondary, taurine-conjugated, and glycine-conjugated BAs in the ileal pool was about 46:15:9:30, whereas it was 28:61:1:11 in the cecum and 22:65:3:9 in the colon (P < 0.05).
30102377	11	90	theme	Greater	1549:1555	arg1	expression					1557:1566	Greater expression	1549:1566	Greater expression of G protein-coupled bile acid receptor and multidrug resistance protein 3 (MRP3)	1549:1648	Greater expression of G protein-coupled bile acid receptor and multidrug resistance protein 3 (MRP3) was found in the cecum and colon (P < 0.05).
30102377	9	91	theme	farnesoid	1183:1191	arg1	FXR					1205:1207	FXR	1205:1207	FXR	1205:1207	Overall, the ileum had the greatest expression of farnesoid X receptor (FXR) and apical sodium-coupled bile acid transporter (ASBT) than the duodenum, jejunum, cecum, and colon (P < 0.05), whereas organic solute transporters α/β (OSTα/β) gene expression was peaked in the ileum and jejunum (P < 0.05).
30102377	9	91	theme	farnesoid	1183:1191	arg1	receptor					1195:1202	farnesoid X receptor	1183:1202	farnesoid X receptor (FXR)	1183:1208	Overall, the ileum had the greatest expression of farnesoid X receptor (FXR) and apical sodium-coupled bile acid transporter (ASBT) than the duodenum, jejunum, cecum, and colon (P < 0.05), whereas organic solute transporters α/β (OSTα/β) gene expression was peaked in the ileum and jejunum (P < 0.05).
30102377	16	92	from	transport	2417:2425	arg1	cecum					2434:2438	the cecum	2430:2438	the cecum	2430:2438	In the ileum, pectin increased in-and-out BA transport on the apical membrane by increasing ASBT and MRP2, but it increased the overall BA transport in the cecum by increasing OSTα/β and MRP3.
30102377	7	93	theme	primary	843:849	arg1	BAs					906:908	primary, secondary, taurine-conjugated, and glycine-conjugated BAs	843:908	primary, secondary, taurine-conjugated, and glycine-conjugated BAs in the ileal pool	843:926	The ratio of primary, secondary, taurine-conjugated, and glycine-conjugated BAs in the ileal pool was about 46:15:9:30, whereas it was 28:61:1:11 in the cecum and 22:65:3:9 in the colon (P < 0.05).
30102377	9	94	theme	receptor	1195:1202	arg1	expression					1169:1178	the greatest expression	1156:1178	the greatest expression of farnesoid X receptor (FXR) and apical sodium-coupled bile acid transporter (ASBT)	1156:1263	Overall, the ileum had the greatest expression of farnesoid X receptor (FXR) and apical sodium-coupled bile acid transporter (ASBT) than the duodenum, jejunum, cecum, and colon (P < 0.05), whereas organic solute transporters α/β (OSTα/β) gene expression was peaked in the ileum and jejunum (P < 0.05).
30102377	14	95	theme	acid-CoA	2004:2011	arg1	synthase					2013:2020	bile acid-CoA synthase	1999:2020	bile acid-CoA synthase	1999:2020	Liver enzymes involved in BA biosynthesis (CYP7A1, CYP27A1, bile acid-CoA synthase, and bile acid-CoA:amino acid N acyltransferase) were not affected by pectin consumption.
30102377	9	96	theme	gene	1371:1374	arg1	expression					1376:1385	organic solute transporters α/β (OSTα/β) gene expression	1330:1385	organic solute transporters α/β (OSTα/β) gene expression	1330:1385	Overall, the ileum had the greatest expression of farnesoid X receptor (FXR) and apical sodium-coupled bile acid transporter (ASBT) than the duodenum, jejunum, cecum, and colon (P < 0.05), whereas organic solute transporters α/β (OSTα/β) gene expression was peaked in the ileum and jejunum (P < 0.05).
30102377	12	97	theme	decreasing	1791:1800	arg1	tendency					1802:1809	a decreasing tendency	1789:1809	a decreasing tendency	1789:1809	In pigs fed with 5% pectin, only cecal UDCA (P = 0.097) and hyocholic acid (P = 0.088) showed a decreasing tendency.
30102377	15	98	theme	BA	2188:2189	arg1	size					2196:2199	the greater BA pool size	2176:2199	the greater BA pool size	2176:2199	In conclusion, the abundant distribution of BA transporters and the greater BA pool size suggests the ileum as the major site for intestinal BA reabsorption in pigs.
30102377	11	99	theme	protein-coupled	1573:1587	arg1	receptor					1599:1606	G protein-coupled bile acid receptor	1571:1606	G protein-coupled bile acid receptor	1571:1606	Greater expression of G protein-coupled bile acid receptor and multidrug resistance protein 3 (MRP3) was found in the cecum and colon (P < 0.05).
30102377	6	100	dep	size	791:794	arg1	the					787:789	the	787:789	the	787:789	Along the length of the intestine, the size and composition of BA pools vary.
30102377	5	101	theme	Serum	620:624	arg1	triglycerides					626:638	Serum triglycerides	620:638	Serum triglycerides	620:638	Serum triglycerides tended to be lower in the pectin-fed animals (P = 0.093), whereas no change was noted in serum total bile acid.
30102377	10	102	theme	multidrug	1446:1454	arg1	MRP2					1478:1481	MRP2	1478:1481	MRP2	1478:1481	Expression multidrug resistance protein 2 (MRP2) gradually decreased towards the end of the intestine (P < 0.05).
30102377	10	102	theme	multidrug	1446:1454	arg1	protein					1467:1473	multidrug resistance protein 2	1446:1475	Expression multidrug resistance protein 2 (MRP2)	1435:1482	Expression multidrug resistance protein 2 (MRP2) gradually decreased towards the end of the intestine (P < 0.05).
30102377	11	103	located	found	1654:1658	arg2	expression					1557:1566	Greater expression	1549:1566	Greater expression of G protein-coupled bile acid receptor and multidrug resistance protein 3 (MRP3)	1549:1648	Greater expression of G protein-coupled bile acid receptor and multidrug resistance protein 3 (MRP3) was found in the cecum and colon (P < 0.05).
30102377	11	103	located	found	1654:1658	arg1	colon					1677:1681	colon	1677:1681	colon	1677:1681	Greater expression of G protein-coupled bile acid receptor and multidrug resistance protein 3 (MRP3) was found in the cecum and colon (P < 0.05).
30102377	11	103	located	found	1654:1658	arg1	<					1686:1686	P < 0.05	1684:1691	P < 0.05	1684:1691	Greater expression of G protein-coupled bile acid receptor and multidrug resistance protein 3 (MRP3) was found in the cecum and colon (P < 0.05).
30102377	11	103	located	found	1654:1658	arg1	cecum					1667:1671	cecum	1667:1671	cecum	1667:1671	Greater expression of G protein-coupled bile acid receptor and multidrug resistance protein 3 (MRP3) was found in the cecum and colon (P < 0.05).
30102377	7	104	from	ratio	834:838	arg1	pool					923:926	the ileal pool	913:926	the ileal pool	913:926	The ratio of primary, secondary, taurine-conjugated, and glycine-conjugated BAs in the ileal pool was about 46:15:9:30, whereas it was 28:61:1:11 in the cecum and 22:65:3:9 in the colon (P < 0.05).
30102377	0	105	theme	bile	69:72	arg1	acids					74:78	intestinal bile acids	58:78	intestinal bile acids	58:78	Effects of dietary pectin on the profile and transport of intestinal bile acids in young pigs.
30102377	9	106	theme	sodium-coupled	1221:1234	arg1	ASBT					1259:1262	ASBT	1259:1262	ASBT	1259:1262	Overall, the ileum had the greatest expression of farnesoid X receptor (FXR) and apical sodium-coupled bile acid transporter (ASBT) than the duodenum, jejunum, cecum, and colon (P < 0.05), whereas organic solute transporters α/β (OSTα/β) gene expression was peaked in the ileum and jejunum (P < 0.05).
30102377	9	106	theme	sodium-coupled	1221:1234	arg1	transporter					1246:1256	apical sodium-coupled bile acid transporter	1214:1256	apical sodium-coupled bile acid transporter (ASBT)	1214:1263	Overall, the ileum had the greatest expression of farnesoid X receptor (FXR) and apical sodium-coupled bile acid transporter (ASBT) than the duodenum, jejunum, cecum, and colon (P < 0.05), whereas organic solute transporters α/β (OSTα/β) gene expression was peaked in the ileum and jejunum (P < 0.05).
30102377	15	107	from	reabsorption	2256:2267	arg1	pigs					2272:2275	pigs	2272:2275	pigs	2272:2275	In conclusion, the abundant distribution of BA transporters and the greater BA pool size suggests the ileum as the major site for intestinal BA reabsorption in pigs.
30102377	6	108	theme	BA	815:816	arg1	pools					818:822	BA pools	815:822	BA pools	815:822	Along the length of the intestine, the size and composition of BA pools vary.
30102377	9	109	theme	acid	1241:1244	arg1	ASBT					1259:1262	ASBT	1259:1262	ASBT	1259:1262	Overall, the ileum had the greatest expression of farnesoid X receptor (FXR) and apical sodium-coupled bile acid transporter (ASBT) than the duodenum, jejunum, cecum, and colon (P < 0.05), whereas organic solute transporters α/β (OSTα/β) gene expression was peaked in the ileum and jejunum (P < 0.05).
30102377	9	109	theme	acid	1241:1244	arg1	transporter					1246:1256	apical sodium-coupled bile acid transporter	1214:1256	apical sodium-coupled bile acid transporter (ASBT)	1214:1263	Overall, the ileum had the greatest expression of farnesoid X receptor (FXR) and apical sodium-coupled bile acid transporter (ASBT) than the duodenum, jejunum, cecum, and colon (P < 0.05), whereas organic solute transporters α/β (OSTα/β) gene expression was peaked in the ileum and jejunum (P < 0.05).
30102377	14	110	theme	BA	1965:1966	arg1	biosynthesis					1968:1979	BA biosynthesis	1965:1979	BA biosynthesis (CYP7A1, CYP27A1, bile acid-CoA synthase, and bile acid-CoA:amino acid N acyltransferase)	1965:2069	Liver enzymes involved in BA biosynthesis (CYP7A1, CYP27A1, bile acid-CoA synthase, and bile acid-CoA:amino acid N acyltransferase) were not affected by pectin consumption.
30102377	3	111	theme	young	310:314	arg1	kg					335:336	11.05 ± 0.11 kg	322:336	11.05 ± 0.11 kg	322:336	Twelve young pigs (11.05 ± 0.11 kg) were randomly divided into 2 groups and fed corn-soybean meal diets with either 5% pectin or cornstarch for 72 d.
30102377	3	111	theme	young	310:314	arg1	pigs					316:319	Twelve young pigs	303:319	Twelve young pigs (11.05 ± 0.11 kg)	303:337	Twelve young pigs (11.05 ± 0.11 kg) were randomly divided into 2 groups and fed corn-soybean meal diets with either 5% pectin or cornstarch for 72 d.
30102377	12	112	theme	=	1742:1742	arg1	P					1740:1740	P = 0.097	1740:1748	P = 0.097	1740:1748	In pigs fed with 5% pectin, only cecal UDCA (P = 0.097) and hyocholic acid (P = 0.088) showed a decreasing tendency.
30102377	12	112	theme	=	1742:1742	arg1	UDCA					1734:1737	only cecal UDCA	1723:1737	only cecal UDCA (P = 0.097)	1723:1749	In pigs fed with 5% pectin, only cecal UDCA (P = 0.097) and hyocholic acid (P = 0.088) showed a decreasing tendency.
30102377	12	113	theme	only	1723:1726	arg1	P					1740:1740	P = 0.097	1740:1748	P = 0.097	1740:1748	In pigs fed with 5% pectin, only cecal UDCA (P = 0.097) and hyocholic acid (P = 0.088) showed a decreasing tendency.
30102377	12	113	theme	only	1723:1726	arg1	UDCA					1734:1737	only cecal UDCA	1723:1737	only cecal UDCA (P = 0.097)	1723:1749	In pigs fed with 5% pectin, only cecal UDCA (P = 0.097) and hyocholic acid (P = 0.088) showed a decreasing tendency.
30102377	9	114	theme	P	1424:1424	arg1	<					1426:1426	P < 0.05	1424:1431	P < 0.05	1424:1431	Overall, the ileum had the greatest expression of farnesoid X receptor (FXR) and apical sodium-coupled bile acid transporter (ASBT) than the duodenum, jejunum, cecum, and colon (P < 0.05), whereas organic solute transporters α/β (OSTα/β) gene expression was peaked in the ileum and jejunum (P < 0.05).
30102377	9	114	theme	P	1424:1424	arg1	ileum					1405:1409	the ileum	1401:1409	the ileum	1401:1409	Overall, the ileum had the greatest expression of farnesoid X receptor (FXR) and apical sodium-coupled bile acid transporter (ASBT) than the duodenum, jejunum, cecum, and colon (P < 0.05), whereas organic solute transporters α/β (OSTα/β) gene expression was peaked in the ileum and jejunum (P < 0.05).
30102377	7	115	from	28:61:1:11	965:974	arg1	22:65:3:9					993:1001	22:65:3:9	993:1001	22:65:3:9	993:1001	The ratio of primary, secondary, taurine-conjugated, and glycine-conjugated BAs in the ileal pool was about 46:15:9:30, whereas it was 28:61:1:11 in the cecum and 22:65:3:9 in the colon (P < 0.05).
30102377	7	115	from	28:61:1:11	965:974	arg1	cecum					983:987	cecum	983:987	cecum	983:987	The ratio of primary, secondary, taurine-conjugated, and glycine-conjugated BAs in the ileal pool was about 46:15:9:30, whereas it was 28:61:1:11 in the cecum and 22:65:3:9 in the colon (P < 0.05).
30102377	7	116	dep	46:15:9:30	938:947	arg1	whereas					950:956	whereas	950:956	whereas	950:956	The ratio of primary, secondary, taurine-conjugated, and glycine-conjugated BAs in the ileal pool was about 46:15:9:30, whereas it was 28:61:1:11 in the cecum and 22:65:3:9 in the colon (P < 0.05).
30102377	5	117	theme	pectin-fed	666:675	arg1	animals					677:683	the pectin-fed animals	662:683	the pectin-fed animals	662:683	Serum triglycerides tended to be lower in the pectin-fed animals (P = 0.093), whereas no change was noted in serum total bile acid.
30102377	12	118	theme	hyocholic	1755:1763	arg1	P					1771:1771	P = 0.088	1771:1779	P = 0.088	1771:1779	In pigs fed with 5% pectin, only cecal UDCA (P = 0.097) and hyocholic acid (P = 0.088) showed a decreasing tendency.
30102377	12	118	theme	hyocholic	1755:1763	arg1	acid					1765:1768	hyocholic acid	1755:1768	hyocholic acid (P = 0.088)	1755:1780	In pigs fed with 5% pectin, only cecal UDCA (P = 0.097) and hyocholic acid (P = 0.088) showed a decreasing tendency.
30102377	13	119	theme	pigs	1922:1925	arg1	cecum					1905:1909	the cecum	1901:1909	the cecum of PEC-fed pigs (P < 0.05)	1901:1936	But FXR, ASBT, and MRP2 were upregulated in the ileum and FXR, OSTα/β, MRP2, and MRP3 in the cecum of PEC-fed pigs (P < 0.05).
30102377	12	120	theme	cecal	1728:1732	arg1	P					1740:1740	P = 0.097	1740:1748	P = 0.097	1740:1748	In pigs fed with 5% pectin, only cecal UDCA (P = 0.097) and hyocholic acid (P = 0.088) showed a decreasing tendency.
30102377	12	120	theme	cecal	1728:1732	arg1	UDCA					1734:1737	only cecal UDCA	1723:1737	only cecal UDCA (P = 0.097)	1723:1749	In pigs fed with 5% pectin, only cecal UDCA (P = 0.097) and hyocholic acid (P = 0.088) showed a decreasing tendency.
30102377	0	121	theme	pectin	19:24	arg1	Effects					0:6	Effects	0:6	Effects of dietary pectin on the profile and transport of intestinal bile acids in young pigs.	0:93	Effects of dietary pectin on the profile and transport of intestinal bile acids in young pigs.
30102377	4	122	dep	increased	598:606	arg1	<					611:611	P < 0.05	609:616	P < 0.05	609:616	In pigs fed with pectin, total cholesterol and low-density lipoprotein cholesterol (LDL-C) were lowered but high-density lipoprotein (HDL-C) was increased (P < 0.05).
30102377	14	123	theme	acid	2047:2050	arg1	acyltransferase					2054:2068	amino acid N acyltransferase	2041:2068	CYP7A1, CYP27A1, bile acid-CoA synthase, and bile acid-CoA:amino acid N acyltransferase	1982:2068	Liver enzymes involved in BA biosynthesis (CYP7A1, CYP27A1, bile acid-CoA synthase, and bile acid-CoA:amino acid N acyltransferase) were not affected by pectin consumption.
30102377	7	124	from	BAs	906:908	arg1	pool					923:926	the ileal pool	913:926	the ileal pool	913:926	The ratio of primary, secondary, taurine-conjugated, and glycine-conjugated BAs in the ileal pool was about 46:15:9:30, whereas it was 28:61:1:11 in the cecum and 22:65:3:9 in the colon (P < 0.05).
30102377	3	125	theme	corn-soybean	383:394	arg1	diets					401:405	corn-soybean meal diets	383:405	corn-soybean meal diets with either 5% pectin or cornstarch for 72 d	383:450	Twelve young pigs (11.05 ± 0.11 kg) were randomly divided into 2 groups and fed corn-soybean meal diets with either 5% pectin or cornstarch for 72 d.
30102377	14	126	theme	pectin	2092:2097	arg1	consumption					2099:2109	pectin consumption	2092:2109	pectin consumption	2092:2109	Liver enzymes involved in BA biosynthesis (CYP7A1, CYP27A1, bile acid-CoA synthase, and bile acid-CoA:amino acid N acyltransferase) were not affected by pectin consumption.
30102377	12	127	theme	%	1713:1713	arg1	pectin					1715:1720	5% pectin	1712:1720	5% pectin	1712:1720	In pigs fed with 5% pectin, only cecal UDCA (P = 0.097) and hyocholic acid (P = 0.088) showed a decreasing tendency.
30102377	7	128	from	pool	923:926	arg1	ratio					834:838	The ratio	830:838	The ratio of primary, secondary, taurine-conjugated, and glycine-conjugated BAs in the ileal pool	830:926	The ratio of primary, secondary, taurine-conjugated, and glycine-conjugated BAs in the ileal pool was about 46:15:9:30, whereas it was 28:61:1:11 in the cecum and 22:65:3:9 in the colon (P < 0.05).
30102377	7	128	from	pool	923:926	arg1	46:15:9:30					938:947	46:15:9:30	938:947	46:15:9:30	938:947	The ratio of primary, secondary, taurine-conjugated, and glycine-conjugated BAs in the ileal pool was about 46:15:9:30, whereas it was 28:61:1:11 in the cecum and 22:65:3:9 in the colon (P < 0.05).
30102377	7	129	dep	cecum	983:987	arg1	the					979:981	the	979:981	the	979:981	The ratio of primary, secondary, taurine-conjugated, and glycine-conjugated BAs in the ileal pool was about 46:15:9:30, whereas it was 28:61:1:11 in the cecum and 22:65:3:9 in the colon (P < 0.05).
30102377	8	130	theme	BA	1124:1125	arg1	pool					1127:1130	the total BA pool	1114:1130	the total BA pool	1114:1130	In the feces, lithocholic acid and ursodeoxycholic acid (UDCA) made up of over 97% of the total BA pool.
30102377	10	131	dep	decreased	1494:1502	arg1	<					1540:1540	P < 0.05	1538:1545	P < 0.05	1538:1545	Expression multidrug resistance protein 2 (MRP2) gradually decreased towards the end of the intestine (P < 0.05).
30102377	11	132	theme	bile	1589:1592	arg1	receptor					1599:1606	G protein-coupled bile acid receptor	1571:1606	G protein-coupled bile acid receptor	1571:1606	Greater expression of G protein-coupled bile acid receptor and multidrug resistance protein 3 (MRP3) was found in the cecum and colon (P < 0.05).
30102377	4	133	theme	lipoprotein	512:522	arg1	LDL-C					537:541	LDL-C	537:541	LDL-C	537:541	In pigs fed with pectin, total cholesterol and low-density lipoprotein cholesterol (LDL-C) were lowered but high-density lipoprotein (HDL-C) was increased (P < 0.05).
30102377	4	133	theme	lipoprotein	512:522	arg1	cholesterol					524:534	low-density lipoprotein cholesterol	500:534	low-density lipoprotein cholesterol (LDL-C)	500:542	In pigs fed with pectin, total cholesterol and low-density lipoprotein cholesterol (LDL-C) were lowered but high-density lipoprotein (HDL-C) was increased (P < 0.05).
30102377	10	134	theme	intestine	1527:1535	arg1	end					1516:1518	the end	1512:1518	the end of the intestine	1512:1535	Expression multidrug resistance protein 2 (MRP2) gradually decreased towards the end of the intestine (P < 0.05).
30102377	14	135	dep	biosynthesis	1968:1979	arg1	synthase					2013:2020	bile acid-CoA synthase	1999:2020	bile acid-CoA synthase	1999:2020	Liver enzymes involved in BA biosynthesis (CYP7A1, CYP27A1, bile acid-CoA synthase, and bile acid-CoA:amino acid N acyltransferase) were not affected by pectin consumption.
30102377	14	135	dep	biosynthesis	1968:1979	arg1	CYP27A1					1990:1996	CYP27A1	1990:1996	CYP27A1	1990:1996	Liver enzymes involved in BA biosynthesis (CYP7A1, CYP27A1, bile acid-CoA synthase, and bile acid-CoA:amino acid N acyltransferase) were not affected by pectin consumption.
30102377	14	135	dep	biosynthesis	1968:1979	arg1	acid-CoA					2032:2039	bile acid-CoA	2027:2039	bile acid-CoA	2027:2039	Liver enzymes involved in BA biosynthesis (CYP7A1, CYP27A1, bile acid-CoA synthase, and bile acid-CoA:amino acid N acyltransferase) were not affected by pectin consumption.
30102377	14	135	dep	biosynthesis	1968:1979	arg1	CYP7A1					1982:1987	CYP7A1	1982:1987	CYP7A1	1982:1987	Liver enzymes involved in BA biosynthesis (CYP7A1, CYP27A1, bile acid-CoA synthase, and bile acid-CoA:amino acid N acyltransferase) were not affected by pectin consumption.
31046875	2	0	theme	Gram-positive	100:112	arg1	actinobacterium					135:149	A Gram-positive, aerobic, non-motile actinobacterium	98:149	A Gram-positive, aerobic, non-motile actinobacterium	98:149	A Gram-positive, aerobic, non-motile actinobacterium, designated YIM 75507T, that was isolated from a soil sample collected from a dry-hot valley, was subjected to a polyphasic taxonomic study.
31046875	11	1	theme	95-96 	1215:1220	arg1	species					1223:1229	95-96 % species	1215:1229	the 95-96 % species circumscription threshold	1211:1255	The draft genome sequence of strain YIM 75507T exhibited low average nucleotide identity to the closest related strain, Sinosporangium album CPCC 201354T (83.97 %), well below the 95-96 % species circumscription threshold.
31046875	10	2	theme	16S	866:868	arg1	sequences					880:888	16S rRNA gene sequences	866:888	16S rRNA gene sequences	866:888	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain YIM 75507T formed a stable and distinct lineage clustered with the genus Sinosporangium in the family Streptosporangiaceae.
31046875	13	3	dep	evidence	1371:1378	arg1	basis					1315:1319	basis	1315:1319	basis	1315:1319	On the basis of morphological, chemotaxonomic and phylogenetic evidence, strain YIM 75507T is assigned to a novel species of a new genus, for which the name Bailinhaonella thermotolerans gen. nov., sp.
31046875	13	3	dep	evidence	1371:1378	arg1	the					1311:1313	the	1311:1313	the	1311:1313	On the basis of morphological, chemotaxonomic and phylogenetic evidence, strain YIM 75507T is assigned to a novel species of a new genus, for which the name Bailinhaonella thermotolerans gen. nov., sp.
31046875	4	4	theme	Clustered	359:367	arg1	chains					375:380	Clustered spore chains	359:380	Clustered spore chains	359:380	Clustered spore chains were borne from aerial mycelium.
31046875	12	5	theme	genomic	1281:1287	arg1	DNA					1289:1291	the genomic DNA	1277:1291	the genomic DNA	1277:1291	The G+C content of the genomic DNA was 73.8 mol%.
31046875	11	6	theme	circumscription	1231:1245	arg1	threshold					1247:1255	the 95-96 % species circumscription threshold	1211:1255	the 95-96 % species circumscription threshold	1211:1255	The draft genome sequence of strain YIM 75507T exhibited low average nucleotide identity to the closest related strain, Sinosporangium album CPCC 201354T (83.97 %), well below the 95-96 % species circumscription threshold.
31046875	13	7	theme	name	1460:1463	arg1	Bailinhaonella					1465:1478	the name Bailinhaonella	1456:1478	the name Bailinhaonella	1456:1478	On the basis of morphological, chemotaxonomic and phylogenetic evidence, strain YIM 75507T is assigned to a novel species of a new genus, for which the name Bailinhaonella thermotolerans gen. nov., sp.
31046875	10	8	theme	strain	904:909	arg1	75507T					915:920	strain YIM 75507T	904:920	strain YIM 75507T	904:920	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain YIM 75507T formed a stable and distinct lineage clustered with the genus Sinosporangium in the family Streptosporangiaceae.
31046875	10	9	theme	YIM	911:913	arg1	75507T					915:920	strain YIM 75507T	904:920	strain YIM 75507T	904:920	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain YIM 75507T formed a stable and distinct lineage clustered with the genus Sinosporangium in the family Streptosporangiaceae.
31046875	6	10	theme	Whole-cell	506:515	arg1	sugars					517:522	Whole-cell sugars	506:522	Whole-cell sugars	506:522	Whole-cell sugars were galactose, mannose, glucosamine, glucose and ribose.
31046875	11	11	theme	strain	1064:1069	arg1	75507T					1075:1080	strain YIM 75507T	1064:1080	strain YIM 75507T	1064:1080	The draft genome sequence of strain YIM 75507T exhibited low average nucleotide identity to the closest related strain, Sinosporangium album CPCC 201354T (83.97 %), well below the 95-96 % species circumscription threshold.
31046875	11	12	theme	CPCC	1176:1179	arg1	strain					1147:1152	the closest related strain	1127:1152	the closest related strain	1127:1152	The draft genome sequence of strain YIM 75507T exhibited low average nucleotide identity to the closest related strain, Sinosporangium album CPCC 201354T (83.97 %), well below the 95-96 % species circumscription threshold.
31046875	11	12	theme	CPCC	1176:1179	arg1	201354T					1181:1187	Sinosporangium album CPCC 201354T	1155:1187	Sinosporangium album CPCC 201354T (83.97 %)	1155:1197	The draft genome sequence of strain YIM 75507T exhibited low average nucleotide identity to the closest related strain, Sinosporangium album CPCC 201354T (83.97 %), well below the 95-96 % species circumscription threshold.
31046875	11	12	theme	CPCC	1176:1179	arg1	%					1196:1196	83.97 %	1190:1196	83.97 %	1190:1196	The draft genome sequence of strain YIM 75507T exhibited low average nucleotide identity to the closest related strain, Sinosporangium album CPCC 201354T (83.97 %), well below the 95-96 % species circumscription threshold.
31046875	15	13	theme	type	1532:1535	arg1	strain					1537:1542	The type strain	1528:1542	The type strain of Bailinhaonella thermotolerans	1528:1575	The type strain of Bailinhaonella thermotolerans is YIM 75507T (=KCTC 49229T=CGMCC 4.7547T).
31046875	15	13	theme	type	1532:1535	arg1	75507T					1584:1589	YIM 75507T	1580:1589	YIM 75507T (=KCTC 49229T=CGMCC 4.7547T)	1580:1618	The type strain of Bailinhaonella thermotolerans is YIM 75507T (=KCTC 49229T=CGMCC 4.7547T).
31046875	11	14	theme	closest	1131:1137	arg1	201354T					1181:1187	Sinosporangium album CPCC 201354T	1155:1187	Sinosporangium album CPCC 201354T (83.97 %)	1155:1197	The draft genome sequence of strain YIM 75507T exhibited low average nucleotide identity to the closest related strain, Sinosporangium album CPCC 201354T (83.97 %), well below the 95-96 % species circumscription threshold.
31046875	11	14	theme	closest	1131:1137	arg1	strain					1147:1152	the closest related strain	1127:1152	the closest related strain	1127:1152	The draft genome sequence of strain YIM 75507T exhibited low average nucleotide identity to the closest related strain, Sinosporangium album CPCC 201354T (83.97 %), well below the 95-96 % species circumscription threshold.
31046875	15	15	theme	YIM	1580:1582	arg1	4.7547T					1611:1617	=KCTC 49229T=CGMCC 4.7547T	1592:1617	=KCTC 49229T=CGMCC 4.7547T	1592:1617	The type strain of Bailinhaonella thermotolerans is YIM 75507T (=KCTC 49229T=CGMCC 4.7547T).
31046875	15	15	theme	YIM	1580:1582	arg1	strain					1537:1542	The type strain	1528:1542	The type strain of Bailinhaonella thermotolerans	1528:1575	The type strain of Bailinhaonella thermotolerans is YIM 75507T (=KCTC 49229T=CGMCC 4.7547T).
31046875	15	15	theme	YIM	1580:1582	arg1	75507T					1584:1589	YIM 75507T	1580:1589	YIM 75507T (=KCTC 49229T=CGMCC 4.7547T)	1580:1618	The type strain of Bailinhaonella thermotolerans is YIM 75507T (=KCTC 49229T=CGMCC 4.7547T).
31046875	4	16	theme	spore	369:373	arg1	chains					375:380	Clustered spore chains	359:380	Clustered spore chains	359:380	Clustered spore chains were borne from aerial mycelium.
31046875	1	17	theme	new	53:55	arg1	member					57:62	a new member	51:62	a new member of the order Streptosporangiales	51:95	nov., a new member of the order Streptosporangiales.
31046875	1	17	theme	new	53:55	arg1	nov.					45:48	nov.	45:48	nov.	45:48	nov., a new member of the order Streptosporangiales.
31046875	13	18	theme	new	1435:1437	arg1	genus					1439:1443	a new genus	1433:1443	a new genus	1433:1443	On the basis of morphological, chemotaxonomic and phylogenetic evidence, strain YIM 75507T is assigned to a novel species of a new genus, for which the name Bailinhaonella thermotolerans gen. nov., sp.
31046875	3	19	theme	branched	311:318	arg1	hyphae					320:325	branched hyphae	311:325	branched hyphae	311:325	The isolate formed branched hyphae and no fragmentation was found.
31046875	7	20	theme	major	586:590	arg1	MK-9					610:613	MK-9	610:613	MK-9	610:613	The major menaquinones were MK-9(H6), MK-9(H8) and MK-10(H6).
31046875	7	20	theme	major	586:590	arg1	menaquinones					592:603	The major menaquinones	582:603	The major menaquinones	582:603	The major menaquinones were MK-9(H6), MK-9(H8) and MK-10(H6).
31046875	11	21	theme	Sinosporangium	1155:1168	arg1	strain					1147:1152	the closest related strain	1127:1152	the closest related strain	1127:1152	The draft genome sequence of strain YIM 75507T exhibited low average nucleotide identity to the closest related strain, Sinosporangium album CPCC 201354T (83.97 %), well below the 95-96 % species circumscription threshold.
31046875	11	21	theme	Sinosporangium	1155:1168	arg1	201354T					1181:1187	Sinosporangium album CPCC 201354T	1155:1187	Sinosporangium album CPCC 201354T (83.97 %)	1155:1197	The draft genome sequence of strain YIM 75507T exhibited low average nucleotide identity to the closest related strain, Sinosporangium album CPCC 201354T (83.97 %), well below the 95-96 % species circumscription threshold.
31046875	11	21	theme	Sinosporangium	1155:1168	arg1	%					1196:1196	83.97 %	1190:1196	83.97 %	1190:1196	The draft genome sequence of strain YIM 75507T exhibited low average nucleotide identity to the closest related strain, Sinosporangium album CPCC 201354T (83.97 %), well below the 95-96 % species circumscription threshold.
31046875	13	22	theme	phylogenetic	1358:1369	arg1	evidence					1371:1378	morphological, chemotaxonomic and phylogenetic evidence	1324:1378	morphological, chemotaxonomic and phylogenetic evidence	1324:1378	On the basis of morphological, chemotaxonomic and phylogenetic evidence, strain YIM 75507T is assigned to a novel species of a new genus, for which the name Bailinhaonella thermotolerans gen. nov., sp.
31046875	2	23	attach	isolated	184:191	arg2	actinobacterium					135:149	A Gram-positive, aerobic, non-motile actinobacterium	98:149	A Gram-positive, aerobic, non-motile actinobacterium	98:149	A Gram-positive, aerobic, non-motile actinobacterium, designated YIM 75507T, that was isolated from a soil sample collected from a dry-hot valley, was subjected to a polyphasic taxonomic study.
31046875	2	23	attach	isolated	184:191	arg1	sample					205:210	a soil sample	198:210	a soil sample collected from a dry-hot valley	198:242	A Gram-positive, aerobic, non-motile actinobacterium, designated YIM 75507T, that was isolated from a soil sample collected from a dry-hot valley, was subjected to a polyphasic taxonomic study.
31046875	13	24	theme	genus	1439:1443	arg1	species					1422:1428	a novel species	1414:1428	a novel species of a new genus, for which the name Bailinhaonella thermotolerans gen. nov., sp	1414:1507	On the basis of morphological, chemotaxonomic and phylogenetic evidence, strain YIM 75507T is assigned to a novel species of a new genus, for which the name Bailinhaonella thermotolerans gen. nov., sp.
31046875	13	25	theme	strain	1381:1386	arg1	75507T					1392:1397	strain YIM 75507T	1381:1397	strain YIM 75507T	1381:1397	On the basis of morphological, chemotaxonomic and phylogenetic evidence, strain YIM 75507T is assigned to a novel species of a new genus, for which the name Bailinhaonella thermotolerans gen. nov., sp.
31046875	2	26	theme	taxonomic	275:283	arg1	study					285:289	a polyphasic taxonomic study	262:289	a polyphasic taxonomic study	262:289	A Gram-positive, aerobic, non-motile actinobacterium, designated YIM 75507T, that was isolated from a soil sample collected from a dry-hot valley, was subjected to a polyphasic taxonomic study.
31046875	5	27	theme	meso-diaminopimelic	480:498	arg1	acid					500:503	meso-diaminopimelic acid	480:503	meso-diaminopimelic acid	480:503	The cell-wall peptidoglycan contained glutamic acid, alanine and meso-diaminopimelic acid.
31046875	11	28	theme	genome	1045:1050	arg1	sequence					1052:1059	The draft genome sequence	1035:1059	The draft genome sequence of strain YIM 75507T	1035:1080	The draft genome sequence of strain YIM 75507T exhibited low average nucleotide identity to the closest related strain, Sinosporangium album CPCC 201354T (83.97 %), well below the 95-96 % species circumscription threshold.
31046875	13	29	theme	chemotaxonomic	1339:1352	arg1	evidence					1371:1378	morphological, chemotaxonomic and phylogenetic evidence	1324:1378	morphological, chemotaxonomic and phylogenetic evidence	1324:1378	On the basis of morphological, chemotaxonomic and phylogenetic evidence, strain YIM 75507T is assigned to a novel species of a new genus, for which the name Bailinhaonella thermotolerans gen. nov., sp.
31046875	15	30	theme	Bailinhaonella	1547:1560	arg1	thermotolerans					1562:1575	Bailinhaonella thermotolerans	1547:1575	Bailinhaonella thermotolerans	1547:1575	The type strain of Bailinhaonella thermotolerans is YIM 75507T (=KCTC 49229T=CGMCC 4.7547T).
31046875	15	31	theme	=KCTC	1592:1596	arg1	4.7547T					1611:1617	=KCTC 49229T=CGMCC 4.7547T	1592:1617	=KCTC 49229T=CGMCC 4.7547T	1592:1617	The type strain of Bailinhaonella thermotolerans is YIM 75507T (=KCTC 49229T=CGMCC 4.7547T).
31046875	15	31	theme	=KCTC	1592:1596	arg1	75507T					1584:1589	YIM 75507T	1580:1589	YIM 75507T (=KCTC 49229T=CGMCC 4.7547T)	1580:1618	The type strain of Bailinhaonella thermotolerans is YIM 75507T (=KCTC 49229T=CGMCC 4.7547T).
31046875	2	32	theme	polyphasic	264:273	arg1	study					285:289	a polyphasic taxonomic study	262:289	a polyphasic taxonomic study	262:289	A Gram-positive, aerobic, non-motile actinobacterium, designated YIM 75507T, that was isolated from a soil sample collected from a dry-hot valley, was subjected to a polyphasic taxonomic study.
31046875	12	33	theme	73.8 mol	1297:1304	arg1	content					1266:1272	The G+C content	1258:1272	The G+C content of the genomic DNA	1258:1291	The G+C content of the genomic DNA was 73.8 mol%.
31046875	12	33	theme	73.8 mol	1297:1304	arg1	%					1305:1305	73.8 mol%	1297:1305	73.8 mol%	1297:1305	The G+C content of the genomic DNA was 73.8 mol%.
31046875	11	34	theme	nucleotide	1104:1113	arg1	identity					1115:1122	low average nucleotide identity	1092:1122	low average nucleotide identity to the closest related strain, Sinosporangium album CPCC 201354T (83.97 %),	1092:1198	The draft genome sequence of strain YIM 75507T exhibited low average nucleotide identity to the closest related strain, Sinosporangium album CPCC 201354T (83.97 %), well below the 95-96 % species circumscription threshold.
31046875	5	35	contain	contained	443:451	arg2	acid					500:503	meso-diaminopimelic acid	480:503	meso-diaminopimelic acid	480:503	The cell-wall peptidoglycan contained glutamic acid, alanine and meso-diaminopimelic acid.
31046875	5	35	contain	contained	443:451	arg1	peptidoglycan					429:441	The cell-wall peptidoglycan	415:441	The cell-wall peptidoglycan	415:441	The cell-wall peptidoglycan contained glutamic acid, alanine and meso-diaminopimelic acid.
31046875	5	35	contain	contained	443:451	arg2	acid					462:465	glutamic acid	453:465	glutamic acid	453:465	The cell-wall peptidoglycan contained glutamic acid, alanine and meso-diaminopimelic acid.
31046875	5	35	contain	contained	443:451	arg2	alanine					468:474	alanine	468:474	alanine	468:474	The cell-wall peptidoglycan contained glutamic acid, alanine and meso-diaminopimelic acid.
31046875	13	36	theme	novel	1416:1420	arg1	species					1422:1428	a novel species	1414:1428	a novel species of a new genus, for which the name Bailinhaonella thermotolerans gen. nov., sp	1414:1507	On the basis of morphological, chemotaxonomic and phylogenetic evidence, strain YIM 75507T is assigned to a novel species of a new genus, for which the name Bailinhaonella thermotolerans gen. nov., sp.
31046875	0	37	theme	gen.	30:33	arg1	sp					41:42	sp	41:42	sp	41:42	Bailinhaonella thermotolerans gen. nov., sp.
31046875	0	37	theme	gen.	30:33	arg1	nov.					35:38	gen. nov.	30:38	gen. nov.	30:38	Bailinhaonella thermotolerans gen. nov., sp.
31046875	8	38	theme	polar	648:652	arg1	phospholipids					654:666	The polar phospholipids	644:666	The polar phospholipids	644:666	The polar phospholipids contained phosphatidylmethylethanolamine, phosphatidylethanolamine and ninhydrin-positive phosphoglycolipid.
31046875	10	39	theme	family	1006:1011	arg1	Streptosporangiaceae					1013:1032	the family Streptosporangiaceae	1002:1032	the family Streptosporangiaceae	1002:1032	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain YIM 75507T formed a stable and distinct lineage clustered with the genus Sinosporangium in the family Streptosporangiaceae.
31046875	1	40	theme	Streptosporangiales	77:95	arg1	member					57:62	a new member	51:62	a new member of the order Streptosporangiales	51:95	nov., a new member of the order Streptosporangiales.
31046875	1	40	theme	Streptosporangiales	77:95	arg1	nov.					45:48	nov.	45:48	nov.	45:48	nov., a new member of the order Streptosporangiales.
31046875	5	41	theme	glutamic	453:460	arg1	acid					462:465	glutamic acid	453:465	glutamic acid	453:465	The cell-wall peptidoglycan contained glutamic acid, alanine and meso-diaminopimelic acid.
31046875	11	42	theme	low	1092:1094	arg1	identity					1115:1122	low average nucleotide identity	1092:1122	low average nucleotide identity to the closest related strain, Sinosporangium album CPCC 201354T (83.97 %),	1092:1198	The draft genome sequence of strain YIM 75507T exhibited low average nucleotide identity to the closest related strain, Sinosporangium album CPCC 201354T (83.97 %), well below the 95-96 % species circumscription threshold.
31046875	13	43	theme	YIM	1388:1390	arg1	75507T					1392:1397	strain YIM 75507T	1381:1397	strain YIM 75507T	1381:1397	On the basis of morphological, chemotaxonomic and phylogenetic evidence, strain YIM 75507T is assigned to a novel species of a new genus, for which the name Bailinhaonella thermotolerans gen. nov., sp.
31046875	2	44	theme	YIM	163:165	arg1	75507T					167:172	YIM 75507T	163:172	YIM 75507T	163:172	A Gram-positive, aerobic, non-motile actinobacterium, designated YIM 75507T, that was isolated from a soil sample collected from a dry-hot valley, was subjected to a polyphasic taxonomic study.
31046875	13	45	theme	gen.	1495:1498	arg1	sp					1506:1507	sp	1506:1507	sp	1506:1507	On the basis of morphological, chemotaxonomic and phylogenetic evidence, strain YIM 75507T is assigned to a novel species of a new genus, for which the name Bailinhaonella thermotolerans gen. nov., sp.
31046875	13	45	theme	gen.	1495:1498	arg1	nov.					1500:1503	gen. nov.	1495:1503	gen. nov.	1495:1503	On the basis of morphological, chemotaxonomic and phylogenetic evidence, strain YIM 75507T is assigned to a novel species of a new genus, for which the name Bailinhaonella thermotolerans gen. nov., sp.
31046875	11	46	theme	YIM	1071:1073	arg1	75507T					1075:1080	strain YIM 75507T	1064:1080	strain YIM 75507T	1064:1080	The draft genome sequence of strain YIM 75507T exhibited low average nucleotide identity to the closest related strain, Sinosporangium album CPCC 201354T (83.97 %), well below the 95-96 % species circumscription threshold.
31046875	13	47	theme	morphological	1324:1336	arg1	evidence					1371:1378	morphological, chemotaxonomic and phylogenetic evidence	1324:1378	morphological, chemotaxonomic and phylogenetic evidence	1324:1378	On the basis of morphological, chemotaxonomic and phylogenetic evidence, strain YIM 75507T is assigned to a novel species of a new genus, for which the name Bailinhaonella thermotolerans gen. nov., sp.
31046875	2	48	theme	soil	200:203	arg1	sample					205:210	a soil sample	198:210	a soil sample collected from a dry-hot valley	198:242	A Gram-positive, aerobic, non-motile actinobacterium, designated YIM 75507T, that was isolated from a soil sample collected from a dry-hot valley, was subjected to a polyphasic taxonomic study.
31046875	11	49	theme	75507T	1075:1080	arg1	sequence					1052:1059	The draft genome sequence	1035:1059	The draft genome sequence of strain YIM 75507T	1035:1080	The draft genome sequence of strain YIM 75507T exhibited low average nucleotide identity to the closest related strain, Sinosporangium album CPCC 201354T (83.97 %), well below the 95-96 % species circumscription threshold.
31046875	11	50	theme	%	1221:1221	arg1	species					1223:1229	95-96 % species	1215:1229	the 95-96 % species circumscription threshold	1211:1255	The draft genome sequence of strain YIM 75507T exhibited low average nucleotide identity to the closest related strain, Sinosporangium album CPCC 201354T (83.97 %), well below the 95-96 % species circumscription threshold.
31046875	11	51	theme	average	1096:1102	arg1	identity					1115:1122	low average nucleotide identity	1092:1122	low average nucleotide identity to the closest related strain, Sinosporangium album CPCC 201354T (83.97 %),	1092:1198	The draft genome sequence of strain YIM 75507T exhibited low average nucleotide identity to the closest related strain, Sinosporangium album CPCC 201354T (83.97 %), well below the 95-96 % species circumscription threshold.
31046875	12	52	theme	G+C	1262:1264	arg1	content					1266:1272	The G+C content	1258:1272	The G+C content of the genomic DNA	1258:1291	The G+C content of the genomic DNA was 73.8 mol%.
31046875	12	52	theme	G+C	1262:1264	arg1	%					1305:1305	73.8 mol%	1297:1305	73.8 mol%	1297:1305	The G+C content of the genomic DNA was 73.8 mol%.
31046875	11	53	theme	species	1223:1229	arg1	threshold					1247:1255	the 95-96 % species circumscription threshold	1211:1255	the 95-96 % species circumscription threshold	1211:1255	The draft genome sequence of strain YIM 75507T exhibited low average nucleotide identity to the closest related strain, Sinosporangium album CPCC 201354T (83.97 %), well below the 95-96 % species circumscription threshold.
31046875	10	54	theme	stable	931:936	arg1	lineage					951:957	a stable and distinct lineage	929:957	a stable and distinct lineage clustered with the genus Sinosporangium in the family Streptosporangiaceae	929:1032	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain YIM 75507T formed a stable and distinct lineage clustered with the genus Sinosporangium in the family Streptosporangiaceae.
31046875	2	55	dep	Gram-positive	100:112	arg1	non-motile					124:133	non-motile	124:133	non-motile	124:133	A Gram-positive, aerobic, non-motile actinobacterium, designated YIM 75507T, that was isolated from a soil sample collected from a dry-hot valley, was subjected to a polyphasic taxonomic study.
31046875	2	55	dep	Gram-positive	100:112	arg1	aerobic					115:121	aerobic	115:121	aerobic	115:121	A Gram-positive, aerobic, non-motile actinobacterium, designated YIM 75507T, that was isolated from a soil sample collected from a dry-hot valley, was subjected to a polyphasic taxonomic study.
31046875	10	56	theme	genus	978:982	arg1	Sinosporangium					984:997	the genus Sinosporangium	974:997	the genus Sinosporangium in the family Streptosporangiaceae	974:1032	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain YIM 75507T formed a stable and distinct lineage clustered with the genus Sinosporangium in the family Streptosporangiaceae.
31046875	2	57	theme	dry-hot	229:235	arg1	valley					237:242	a dry-hot valley	227:242	a dry-hot valley	227:242	A Gram-positive, aerobic, non-motile actinobacterium, designated YIM 75507T, that was isolated from a soil sample collected from a dry-hot valley, was subjected to a polyphasic taxonomic study.
31046875	8	58	contain	contained	668:676	arg2	phosphatidylethanolamine					710:733	phosphatidylethanolamine	710:733	phosphatidylethanolamine	710:733	The polar phospholipids contained phosphatidylmethylethanolamine, phosphatidylethanolamine and ninhydrin-positive phosphoglycolipid.
31046875	8	58	contain	contained	668:676	arg2	phosphatidylmethylethanolamine					678:707	phosphatidylmethylethanolamine	678:707	phosphatidylmethylethanolamine	678:707	The polar phospholipids contained phosphatidylmethylethanolamine, phosphatidylethanolamine and ninhydrin-positive phosphoglycolipid.
31046875	8	58	contain	contained	668:676	arg2	phosphoglycolipid					758:774	ninhydrin-positive phosphoglycolipid	739:774	ninhydrin-positive phosphoglycolipid	739:774	The polar phospholipids contained phosphatidylmethylethanolamine, phosphatidylethanolamine and ninhydrin-positive phosphoglycolipid.
31046875	8	58	contain	contained	668:676	arg1	phospholipids					654:666	The polar phospholipids	644:666	The polar phospholipids	644:666	The polar phospholipids contained phosphatidylmethylethanolamine, phosphatidylethanolamine and ninhydrin-positive phosphoglycolipid.
31046875	10	59	theme	Phylogenetic	835:846	arg1	analysis					848:855	Phylogenetic analysis	835:855	Phylogenetic analysis based on 16S rRNA gene sequences	835:888	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain YIM 75507T formed a stable and distinct lineage clustered with the genus Sinosporangium in the family Streptosporangiaceae.
31046875	1	60	theme	order	71:75	arg1	Streptosporangiales					77:95	the order Streptosporangiales	67:95	the order Streptosporangiales	67:95	nov., a new member of the order Streptosporangiales.
31046875	10	61	theme	distinct	942:949	arg1	lineage					951:957	a stable and distinct lineage	929:957	a stable and distinct lineage clustered with the genus Sinosporangium in the family Streptosporangiaceae	929:1032	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain YIM 75507T formed a stable and distinct lineage clustered with the genus Sinosporangium in the family Streptosporangiaceae.
31046875	11	62	theme	related	1139:1145	arg1	201354T					1181:1187	Sinosporangium album CPCC 201354T	1155:1187	Sinosporangium album CPCC 201354T (83.97 %)	1155:1197	The draft genome sequence of strain YIM 75507T exhibited low average nucleotide identity to the closest related strain, Sinosporangium album CPCC 201354T (83.97 %), well below the 95-96 % species circumscription threshold.
31046875	11	62	theme	related	1139:1145	arg1	strain					1147:1152	the closest related strain	1127:1152	the closest related strain	1127:1152	The draft genome sequence of strain YIM 75507T exhibited low average nucleotide identity to the closest related strain, Sinosporangium album CPCC 201354T (83.97 %), well below the 95-96 % species circumscription threshold.
31046875	4	63	theme	aerial	398:403	arg1	mycelium					405:412	aerial mycelium	398:412	aerial mycelium	398:412	Clustered spore chains were borne from aerial mycelium.
31046875	9	64	theme	Major	777:781	arg1	acids					789:793	Major fatty acids	777:793	Major fatty acids	777:793	Major fatty acids were iso-C16 : 0 and 10-methyl-C17 : 0.
31046875	11	65	theme	album	1170:1174	arg1	strain					1147:1152	the closest related strain	1127:1152	the closest related strain	1127:1152	The draft genome sequence of strain YIM 75507T exhibited low average nucleotide identity to the closest related strain, Sinosporangium album CPCC 201354T (83.97 %), well below the 95-96 % species circumscription threshold.
31046875	11	65	theme	album	1170:1174	arg1	201354T					1181:1187	Sinosporangium album CPCC 201354T	1155:1187	Sinosporangium album CPCC 201354T (83.97 %)	1155:1197	The draft genome sequence of strain YIM 75507T exhibited low average nucleotide identity to the closest related strain, Sinosporangium album CPCC 201354T (83.97 %), well below the 95-96 % species circumscription threshold.
31046875	11	65	theme	album	1170:1174	arg1	%					1196:1196	83.97 %	1190:1196	83.97 %	1190:1196	The draft genome sequence of strain YIM 75507T exhibited low average nucleotide identity to the closest related strain, Sinosporangium album CPCC 201354T (83.97 %), well below the 95-96 % species circumscription threshold.
31046875	11	66	theme	draft	1039:1043	arg1	sequence					1052:1059	The draft genome sequence	1035:1059	The draft genome sequence of strain YIM 75507T	1035:1080	The draft genome sequence of strain YIM 75507T exhibited low average nucleotide identity to the closest related strain, Sinosporangium album CPCC 201354T (83.97 %), well below the 95-96 % species circumscription threshold.
31046875	15	67	theme	49229T=CGMCC	1598:1609	arg1	4.7547T					1611:1617	=KCTC 49229T=CGMCC 4.7547T	1592:1617	=KCTC 49229T=CGMCC 4.7547T	1592:1617	The type strain of Bailinhaonella thermotolerans is YIM 75507T (=KCTC 49229T=CGMCC 4.7547T).
31046875	15	67	theme	49229T=CGMCC	1598:1609	arg1	75507T					1584:1589	YIM 75507T	1580:1589	YIM 75507T (=KCTC 49229T=CGMCC 4.7547T)	1580:1618	The type strain of Bailinhaonella thermotolerans is YIM 75507T (=KCTC 49229T=CGMCC 4.7547T).
31046875	12	68	theme	DNA	1289:1291	arg1	content					1266:1272	The G+C content	1258:1272	The G+C content of the genomic DNA	1258:1291	The G+C content of the genomic DNA was 73.8 mol%.
31046875	12	68	theme	DNA	1289:1291	arg1	%					1305:1305	73.8 mol%	1297:1305	73.8 mol%	1297:1305	The G+C content of the genomic DNA was 73.8 mol%.
31046875	9	69	theme	fatty	783:787	arg1	acids					789:793	Major fatty acids	777:793	Major fatty acids	777:793	Major fatty acids were iso-C16 : 0 and 10-methyl-C17 : 0.
31046875	15	70	theme	thermotolerans	1562:1575	arg1	strain					1537:1542	The type strain	1528:1542	The type strain of Bailinhaonella thermotolerans	1528:1575	The type strain of Bailinhaonella thermotolerans is YIM 75507T (=KCTC 49229T=CGMCC 4.7547T).
31046875	15	70	theme	thermotolerans	1562:1575	arg1	75507T					1584:1589	YIM 75507T	1580:1589	YIM 75507T (=KCTC 49229T=CGMCC 4.7547T)	1580:1618	The type strain of Bailinhaonella thermotolerans is YIM 75507T (=KCTC 49229T=CGMCC 4.7547T).
31046875	10	71	theme	gene	875:878	arg1	sequences					880:888	16S rRNA gene sequences	866:888	16S rRNA gene sequences	866:888	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain YIM 75507T formed a stable and distinct lineage clustered with the genus Sinosporangium in the family Streptosporangiaceae.
31046875	5	72	theme	cell-wall	419:427	arg1	peptidoglycan					429:441	The cell-wall peptidoglycan	415:441	The cell-wall peptidoglycan	415:441	The cell-wall peptidoglycan contained glutamic acid, alanine and meso-diaminopimelic acid.
31046875	10	73	from	Sinosporangium	984:997	arg1	Streptosporangiaceae					1013:1032	the family Streptosporangiaceae	1002:1032	the family Streptosporangiaceae	1002:1032	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain YIM 75507T formed a stable and distinct lineage clustered with the genus Sinosporangium in the family Streptosporangiaceae.
31046875	9	74	dep	iso-C16 	800:807	arg1	10-methyl-C17 					816:829	10-methyl-C17 	816:829	10-methyl-C17 	816:829	Major fatty acids were iso-C16 : 0 and 10-methyl-C17 : 0.
31046875	9	74	dep	iso-C16 	800:807	arg1	 0					809:810	 0	809:810	 0	809:810	Major fatty acids were iso-C16 : 0 and 10-methyl-C17 : 0.
31046875	9	74	dep	iso-C16 	800:807	arg1	 0					831:832	 0	831:832	 0	831:832	Major fatty acids were iso-C16 : 0 and 10-methyl-C17 : 0.
31046875	10	75	theme	rRNA	870:873	arg1	sequences					880:888	16S rRNA gene sequences	866:888	16S rRNA gene sequences	866:888	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain YIM 75507T formed a stable and distinct lineage clustered with the genus Sinosporangium in the family Streptosporangiaceae.
31046875	8	76	theme	ninhydrin-positive	739:756	arg1	phosphoglycolipid					758:774	ninhydrin-positive phosphoglycolipid	739:774	ninhydrin-positive phosphoglycolipid	739:774	The polar phospholipids contained phosphatidylmethylethanolamine, phosphatidylethanolamine and ninhydrin-positive phosphoglycolipid.
30124399	7	0	dep	Acetivibrio	1010:1020	arg1	cellulolyticus					1022:1035	Acetivibrio cellulolyticus	1010:1035	Acetivibrio cellulolyticus	1010:1035	Phylogenetic analysis of 16S rRNA gene sequences showed that strain SK-Y3T belongs to clostridial cluster III, exhibiting 91-92% sequence similarity to the most closely related species, namely Clostridium clariflavum, Clostridium straminisolvens and Acetivibrio cellulolyticus.
30124399	8	1	from	taxa	1133:1136	arg1	differences					1087:1097	distinct physiological and phylogenetic differences	1047:1097	distinct physiological and phylogenetic differences from the aforementioned described taxa	1047:1136	Based on distinct physiological and phylogenetic differences from the aforementioned described taxa, strain SK-Y3T (=DSM 103557T=ACCC 19952T) is proposed as the type strain of a novel species of a new genus, Petroclostridium xylanilyticum gen. nov., sp.
30124399	10	2	theme	ribosomal	1342:1350	arg1	protein					1352:1358	ribosomal protein	1342:1358	ribosomal protein	1342:1358	Furthermore, analysis through 16S rRNA gene, ribosomal protein and whole genome sequences indicated that clostridial cluster III members should be reclassified into four novel genera for which the names Hungateiclostridium gen. nov., Thermoclostridium gen. nov., Ruminiclostridium gen. nov. and Pseudoclostridium gen. nov. are proposed.
30124399	10	3	theme	cluster	1414:1420	arg1	members					1426:1432	clostridial cluster III members	1402:1432	clostridial cluster III members	1402:1432	Furthermore, analysis through 16S rRNA gene, ribosomal protein and whole genome sequences indicated that clostridial cluster III members should be reclassified into four novel genera for which the names Hungateiclostridium gen. nov., Thermoclostridium gen. nov., Ruminiclostridium gen. nov. and Pseudoclostridium gen. nov. are proposed.
30124399	8	4	theme	new	1235:1237	arg1	nov.					1282:1285	Petroclostridium xylanilyticum gen. nov.	1246:1285	Petroclostridium xylanilyticum gen. nov.	1246:1285	Based on distinct physiological and phylogenetic differences from the aforementioned described taxa, strain SK-Y3T (=DSM 103557T=ACCC 19952T) is proposed as the type strain of a novel species of a new genus, Petroclostridium xylanilyticum gen. nov., sp.
30124399	8	4	theme	new	1235:1237	arg1	genus					1239:1243	a new genus	1233:1243	a new genus	1233:1243	Based on distinct physiological and phylogenetic differences from the aforementioned described taxa, strain SK-Y3T (=DSM 103557T=ACCC 19952T) is proposed as the type strain of a novel species of a new genus, Petroclostridium xylanilyticum gen. nov., sp.
30124399	7	5	theme	16S	785:787	arg1	sequences					799:807	16S rRNA gene sequences	785:807	16S rRNA gene sequences	785:807	Phylogenetic analysis of 16S rRNA gene sequences showed that strain SK-Y3T belongs to clostridial cluster III, exhibiting 91-92% sequence similarity to the most closely related species, namely Clostridium clariflavum, Clostridium straminisolvens and Acetivibrio cellulolyticus.
30124399	8	6	theme	physiological	1056:1068	arg1	differences					1087:1097	distinct physiological and phylogenetic differences	1047:1097	distinct physiological and phylogenetic differences from the aforementioned described taxa	1047:1136	Based on distinct physiological and phylogenetic differences from the aforementioned described taxa, strain SK-Y3T (=DSM 103557T=ACCC 19952T) is proposed as the type strain of a novel species of a new genus, Petroclostridium xylanilyticum gen. nov., sp.
30124399	1	7	theme	members	155:161	arg1	reclassification					111:126	reclassification	111:126	reclassification	111:126	nov., a xylan-degrading bacterium isolated from an oilfield, and reclassification of clostridial cluster III members into four novel genera in a new Hungateiclostridiaceae fam.
30124399	1	7	theme	members	155:161	arg1	bacterium					70:78	a xylan-degrading bacterium	52:78	a xylan-degrading bacterium isolated from an oilfield	52:104	nov., a xylan-degrading bacterium isolated from an oilfield, and reclassification of clostridial cluster III members into four novel genera in a new Hungateiclostridiaceae fam.
30124399	1	7	theme	members	155:161	arg1	nov.					46:49	nov.	46:49	nov.	46:49	nov., a xylan-degrading bacterium isolated from an oilfield, and reclassification of clostridial cluster III members into four novel genera in a new Hungateiclostridiaceae fam.
30124399	7	8	theme	Phylogenetic	760:771	arg1	analysis					773:780	Phylogenetic analysis	760:780	Phylogenetic analysis of 16S rRNA gene sequences	760:807	Phylogenetic analysis of 16S rRNA gene sequences showed that strain SK-Y3T belongs to clostridial cluster III, exhibiting 91-92% sequence similarity to the most closely related species, namely Clostridium clariflavum, Clostridium straminisolvens and Acetivibrio cellulolyticus.
30124399	11	9	theme	order	1871:1875	arg1	Clostridiales					1877:1889	the order Clostridiales	1867:1889	the order Clostridiales but that was clearly distinguished from other Ruminococcaceae members, which is proposed as a novel family, Hungateiclostridiaceae fam	1867:2024	In combination with the genera Anaerobacterium, Cellulosibacter, Ercella, Fastidiosipila, Mageeibacillus, Pseudobacteroides, Petroclostridium and Saccharofermentans, clostridial cluster III members formed a monophyletic clade within the order Clostridiales but that was clearly distinguished from other Ruminococcaceae members, which is proposed as a novel family, Hungateiclostridiaceae fam.
30124399	7	10	theme	91-92	882:886	arg1	%					887:887	%	887:887	%	887:887	Phylogenetic analysis of 16S rRNA gene sequences showed that strain SK-Y3T belongs to clostridial cluster III, exhibiting 91-92% sequence similarity to the most closely related species, namely Clostridium clariflavum, Clostridium straminisolvens and Acetivibrio cellulolyticus.
30124399	5	11	theme	SK-Y3T	499:504	arg1	iso-C15 					511:518	iso-C15 	511:518	iso-C15 	511:518	The predominant cellular fatty acids of strain SK-Y3T were iso-C15 : 0, anteiso-C15 : 0 and iso-C17 : 0, and the main polar lipids were glycolipids (GL), lipids (L), phosphatidylglycerol (PG) and diphosphatidylglycerol (DPG); no respiratory quinones were detected.
30124399	5	11	theme	SK-Y3T	499:504	arg1	acids					483:487	The predominant cellular fatty acids	452:487	The predominant cellular fatty acids of strain SK-Y3T	452:504	The predominant cellular fatty acids of strain SK-Y3T were iso-C15 : 0, anteiso-C15 : 0 and iso-C17 : 0, and the main polar lipids were glycolipids (GL), lipids (L), phosphatidylglycerol (PG) and diphosphatidylglycerol (DPG); no respiratory quinones were detected.
30124399	8	12	theme	genus	1239:1243	arg1	species					1222:1228	a novel species	1214:1228	a novel species of a new genus, Petroclostridium xylanilyticum gen. nov., sp	1214:1289	Based on distinct physiological and phylogenetic differences from the aforementioned described taxa, strain SK-Y3T (=DSM 103557T=ACCC 19952T) is proposed as the type strain of a novel species of a new genus, Petroclostridium xylanilyticum gen. nov., sp.
30124399	5	13	theme	cellular	468:475	arg1	iso-C15 					511:518	iso-C15 	511:518	iso-C15 	511:518	The predominant cellular fatty acids of strain SK-Y3T were iso-C15 : 0, anteiso-C15 : 0 and iso-C17 : 0, and the main polar lipids were glycolipids (GL), lipids (L), phosphatidylglycerol (PG) and diphosphatidylglycerol (DPG); no respiratory quinones were detected.
30124399	5	13	theme	cellular	468:475	arg1	acids					483:487	The predominant cellular fatty acids	452:487	The predominant cellular fatty acids of strain SK-Y3T	452:504	The predominant cellular fatty acids of strain SK-Y3T were iso-C15 : 0, anteiso-C15 : 0 and iso-C17 : 0, and the main polar lipids were glycolipids (GL), lipids (L), phosphatidylglycerol (PG) and diphosphatidylglycerol (DPG); no respiratory quinones were detected.
30124399	8	14	theme	described	1123:1131	arg1	taxa					1133:1136	the aforementioned described taxa	1104:1136	the aforementioned described taxa	1104:1136	Based on distinct physiological and phylogenetic differences from the aforementioned described taxa, strain SK-Y3T (=DSM 103557T=ACCC 19952T) is proposed as the type strain of a novel species of a new genus, Petroclostridium xylanilyticum gen. nov., sp.
30124399	4	15	theme	NaCl	446:449	arg1	addition					434:441	addition	434:441	addition of NaCl	434:449	Optimum growth occurred at 50 °C, at pH 7.5 and without addition of NaCl.
30124399	10	16	theme	genome	1370:1375	arg1	sequences					1377:1385	whole genome sequences	1364:1385	whole genome sequences	1364:1385	Furthermore, analysis through 16S rRNA gene, ribosomal protein and whole genome sequences indicated that clostridial cluster III members should be reclassified into four novel genera for which the names Hungateiclostridium gen. nov., Thermoclostridium gen. nov., Ruminiclostridium gen. nov. and Pseudoclostridium gen. nov. are proposed.
30124399	7	17	theme	clostridial	846:856	arg1	cluster					858:864	clostridial cluster III	846:868	clostridial cluster III	846:868	Phylogenetic analysis of 16S rRNA gene sequences showed that strain SK-Y3T belongs to clostridial cluster III, exhibiting 91-92% sequence similarity to the most closely related species, namely Clostridium clariflavum, Clostridium straminisolvens and Acetivibrio cellulolyticus.
30124399	1	18	theme	xylan-degrading	54:68	arg1	bacterium					70:78	a xylan-degrading bacterium	52:78	a xylan-degrading bacterium isolated from an oilfield	52:104	nov., a xylan-degrading bacterium isolated from an oilfield, and reclassification of clostridial cluster III members into four novel genera in a new Hungateiclostridiaceae fam.
30124399	1	18	theme	xylan-degrading	54:68	arg1	nov.					46:49	nov.	46:49	nov.	46:49	nov., a xylan-degrading bacterium isolated from an oilfield, and reclassification of clostridial cluster III members into four novel genera in a new Hungateiclostridiaceae fam.
30124399	5	19	theme	fatty	477:481	arg1	iso-C15 					511:518	iso-C15 	511:518	iso-C15 	511:518	The predominant cellular fatty acids of strain SK-Y3T were iso-C15 : 0, anteiso-C15 : 0 and iso-C17 : 0, and the main polar lipids were glycolipids (GL), lipids (L), phosphatidylglycerol (PG) and diphosphatidylglycerol (DPG); no respiratory quinones were detected.
30124399	5	19	theme	fatty	477:481	arg1	acids					483:487	The predominant cellular fatty acids	452:487	The predominant cellular fatty acids of strain SK-Y3T	452:504	The predominant cellular fatty acids of strain SK-Y3T were iso-C15 : 0, anteiso-C15 : 0 and iso-C17 : 0, and the main polar lipids were glycolipids (GL), lipids (L), phosphatidylglycerol (PG) and diphosphatidylglycerol (DPG); no respiratory quinones were detected.
30124399	10	20	theme	novel	1467:1471	arg1	genera					1473:1478	four novel genera	1462:1478	four novel genera for which the names Hungateiclostridium gen. nov., Thermoclostridium gen. nov., Ruminiclostridium gen. nov. and Pseudoclostridium gen. nov. are proposed	1462:1631	Furthermore, analysis through 16S rRNA gene, ribosomal protein and whole genome sequences indicated that clostridial cluster III members should be reclassified into four novel genera for which the names Hungateiclostridium gen. nov., Thermoclostridium gen. nov., Ruminiclostridium gen. nov. and Pseudoclostridium gen. nov. are proposed.
30124399	5	21	theme	main	565:568	arg1	glycolipids					588:598	glycolipids	588:598	glycolipids (GL)	588:603	The predominant cellular fatty acids of strain SK-Y3T were iso-C15 : 0, anteiso-C15 : 0 and iso-C17 : 0, and the main polar lipids were glycolipids (GL), lipids (L), phosphatidylglycerol (PG) and diphosphatidylglycerol (DPG); no respiratory quinones were detected.
30124399	5	21	theme	main	565:568	arg1	lipids					576:581	the main polar lipids	561:581	the main polar lipids	561:581	The predominant cellular fatty acids of strain SK-Y3T were iso-C15 : 0, anteiso-C15 : 0 and iso-C17 : 0, and the main polar lipids were glycolipids (GL), lipids (L), phosphatidylglycerol (PG) and diphosphatidylglycerol (DPG); no respiratory quinones were detected.
30124399	10	22	theme	16S	1327:1329	arg1	rRNA					1331:1334	16S rRNA	1327:1334	16S rRNA gene	1327:1339	Furthermore, analysis through 16S rRNA gene, ribosomal protein and whole genome sequences indicated that clostridial cluster III members should be reclassified into four novel genera for which the names Hungateiclostridium gen. nov., Thermoclostridium gen. nov., Ruminiclostridium gen. nov. and Pseudoclostridium gen. nov. are proposed.
30124399	7	23	theme	%	887:887	arg1	similarity					898:907	91-92% sequence similarity	882:907	91-92% sequence similarity to the most closely related species, namely Clostridium clariflavum, Clostridium straminisolvens and Acetivibrio cellulolyticus	882:1035	Phylogenetic analysis of 16S rRNA gene sequences showed that strain SK-Y3T belongs to clostridial cluster III, exhibiting 91-92% sequence similarity to the most closely related species, namely Clostridium clariflavum, Clostridium straminisolvens and Acetivibrio cellulolyticus.
30124399	8	24	theme	103557T=ACCC	1159:1170	arg1	SK-Y3T					1146:1151	strain SK-Y3T	1139:1151	strain SK-Y3T (=DSM 103557T=ACCC 19952T)	1139:1178	Based on distinct physiological and phylogenetic differences from the aforementioned described taxa, strain SK-Y3T (=DSM 103557T=ACCC 19952T) is proposed as the type strain of a novel species of a new genus, Petroclostridium xylanilyticum gen. nov., sp.
30124399	8	24	theme	103557T=ACCC	1159:1170	arg1	19952T					1172:1177	=DSM 103557T=ACCC 19952T	1154:1177	=DSM 103557T=ACCC 19952T	1154:1177	Based on distinct physiological and phylogenetic differences from the aforementioned described taxa, strain SK-Y3T (=DSM 103557T=ACCC 19952T) is proposed as the type strain of a novel species of a new genus, Petroclostridium xylanilyticum gen. nov., sp.
30124399	0	25	theme	xylanilyticum	17:29	arg1	nov.					36:39	Petroclostridium xylanilyticum gen. nov.	0:39	Petroclostridium xylanilyticum gen. nov.	0:39	Petroclostridium xylanilyticum gen. nov., sp.
30124399	5	26	theme	polar	570:574	arg1	glycolipids					588:598	glycolipids	588:598	glycolipids (GL)	588:603	The predominant cellular fatty acids of strain SK-Y3T were iso-C15 : 0, anteiso-C15 : 0 and iso-C17 : 0, and the main polar lipids were glycolipids (GL), lipids (L), phosphatidylglycerol (PG) and diphosphatidylglycerol (DPG); no respiratory quinones were detected.
30124399	5	26	theme	polar	570:574	arg1	lipids					576:581	the main polar lipids	561:581	the main polar lipids	561:581	The predominant cellular fatty acids of strain SK-Y3T were iso-C15 : 0, anteiso-C15 : 0 and iso-C17 : 0, and the main polar lipids were glycolipids (GL), lipids (L), phosphatidylglycerol (PG) and diphosphatidylglycerol (DPG); no respiratory quinones were detected.
30124399	10	27	theme	rRNA	1331:1334	arg1	gene					1336:1339	16S rRNA gene	1327:1339	16S rRNA gene	1327:1339	Furthermore, analysis through 16S rRNA gene, ribosomal protein and whole genome sequences indicated that clostridial cluster III members should be reclassified into four novel genera for which the names Hungateiclostridium gen. nov., Thermoclostridium gen. nov., Ruminiclostridium gen. nov. and Pseudoclostridium gen. nov. are proposed.
30124399	3	28	attach	isolated	324:331	arg2	SK-Y3T					312:317	SK-Y3T	312:317	SK-Y3T	312:317	A rod-shaped, Gram-stain-positive, obligately anaerobic, xylan-degrading bacterium, SK-Y3T, was isolated from oily-sludge of Shengli oilfield, China.
30124399	3	28	attach	isolated	324:331	arg2	bacterium					301:309	A rod-shaped, Gram-stain-positive, obligately anaerobic, xylan-degrading bacterium	228:309	bacterium	301:309	A rod-shaped, Gram-stain-positive, obligately anaerobic, xylan-degrading bacterium, SK-Y3T, was isolated from oily-sludge of Shengli oilfield, China.
30124399	3	28	attach	isolated	324:331	arg1	oily-sludge					338:348	oily-sludge	338:348	oily-sludge of Shengli oilfield, China	338:375	A rod-shaped, Gram-stain-positive, obligately anaerobic, xylan-degrading bacterium, SK-Y3T, was isolated from oily-sludge of Shengli oilfield, China.
30124399	1	29	theme	novel	173:177	arg1	genera					179:184	four novel genera	168:184	four novel genera in a new Hungateiclostridiaceae fam	168:220	nov., a xylan-degrading bacterium isolated from an oilfield, and reclassification of clostridial cluster III members into four novel genera in a new Hungateiclostridiaceae fam.
30124399	0	30	theme	Petroclostridium	0:15	arg1	nov.					36:39	Petroclostridium xylanilyticum gen. nov.	0:39	Petroclostridium xylanilyticum gen. nov.	0:39	Petroclostridium xylanilyticum gen. nov., sp.
30124399	3	31	theme	rod-shaped	230:239	arg1	bacterium					301:309	A rod-shaped, Gram-stain-positive, obligately anaerobic, xylan-degrading bacterium	228:309	bacterium	301:309	A rod-shaped, Gram-stain-positive, obligately anaerobic, xylan-degrading bacterium, SK-Y3T, was isolated from oily-sludge of Shengli oilfield, China.
30124399	3	31	theme	rod-shaped	230:239	arg1	SK-Y3T					312:317	SK-Y3T	312:317	SK-Y3T	312:317	A rod-shaped, Gram-stain-positive, obligately anaerobic, xylan-degrading bacterium, SK-Y3T, was isolated from oily-sludge of Shengli oilfield, China.
30124399	4	32	theme	Optimum	378:384	arg1	growth					386:391	Optimum growth	378:391	Optimum growth	378:391	Optimum growth occurred at 50 °C, at pH 7.5 and without addition of NaCl.
30124399	8	33	theme	gen.	1277:1280	arg1	nov.					1282:1285	Petroclostridium xylanilyticum gen. nov.	1246:1285	Petroclostridium xylanilyticum gen. nov.	1246:1285	Based on distinct physiological and phylogenetic differences from the aforementioned described taxa, strain SK-Y3T (=DSM 103557T=ACCC 19952T) is proposed as the type strain of a novel species of a new genus, Petroclostridium xylanilyticum gen. nov., sp.
30124399	8	33	theme	gen.	1277:1280	arg1	genus					1239:1243	a new genus	1233:1243	a new genus	1233:1243	Based on distinct physiological and phylogenetic differences from the aforementioned described taxa, strain SK-Y3T (=DSM 103557T=ACCC 19952T) is proposed as the type strain of a novel species of a new genus, Petroclostridium xylanilyticum gen. nov., sp.
30124399	8	33	theme	gen.	1277:1280	arg1	sp					1288:1289	sp	1288:1289	sp	1288:1289	Based on distinct physiological and phylogenetic differences from the aforementioned described taxa, strain SK-Y3T (=DSM 103557T=ACCC 19952T) is proposed as the type strain of a novel species of a new genus, Petroclostridium xylanilyticum gen. nov., sp.
30124399	11	34	theme	novel	1985:1989	arg1	family					1991:1996	a novel family	1983:1996	a novel family	1983:1996	In combination with the genera Anaerobacterium, Cellulosibacter, Ercella, Fastidiosipila, Mageeibacillus, Pseudobacteroides, Petroclostridium and Saccharofermentans, clostridial cluster III members formed a monophyletic clade within the order Clostridiales but that was clearly distinguished from other Ruminococcaceae members, which is proposed as a novel family, Hungateiclostridiaceae fam.
30124399	11	34	theme	novel	1985:1989	arg1	fam					2022:2024	Hungateiclostridiaceae fam	1999:2024	Hungateiclostridiaceae fam	1999:2024	In combination with the genera Anaerobacterium, Cellulosibacter, Ercella, Fastidiosipila, Mageeibacillus, Pseudobacteroides, Petroclostridium and Saccharofermentans, clostridial cluster III members formed a monophyletic clade within the order Clostridiales but that was clearly distinguished from other Ruminococcaceae members, which is proposed as a novel family, Hungateiclostridiaceae fam.
30124399	11	34	theme	novel	1985:1989	arg1	members					1953:1959	other Ruminococcaceae members	1931:1959	other Ruminococcaceae members	1931:1959	In combination with the genera Anaerobacterium, Cellulosibacter, Ercella, Fastidiosipila, Mageeibacillus, Pseudobacteroides, Petroclostridium and Saccharofermentans, clostridial cluster III members formed a monophyletic clade within the order Clostridiales but that was clearly distinguished from other Ruminococcaceae members, which is proposed as a novel family, Hungateiclostridiaceae fam.
30124399	3	35	theme	anaerobic	274:282	arg1	bacterium					301:309	A rod-shaped, Gram-stain-positive, obligately anaerobic, xylan-degrading bacterium	228:309	bacterium	301:309	A rod-shaped, Gram-stain-positive, obligately anaerobic, xylan-degrading bacterium, SK-Y3T, was isolated from oily-sludge of Shengli oilfield, China.
30124399	3	35	theme	anaerobic	274:282	arg1	SK-Y3T					312:317	SK-Y3T	312:317	SK-Y3T	312:317	A rod-shaped, Gram-stain-positive, obligately anaerobic, xylan-degrading bacterium, SK-Y3T, was isolated from oily-sludge of Shengli oilfield, China.
30124399	7	36	theme	strain	821:826	arg1	SK-Y3T					828:833	strain SK-Y3T	821:833	strain SK-Y3T	821:833	Phylogenetic analysis of 16S rRNA gene sequences showed that strain SK-Y3T belongs to clostridial cluster III, exhibiting 91-92% sequence similarity to the most closely related species, namely Clostridium clariflavum, Clostridium straminisolvens and Acetivibrio cellulolyticus.
30124399	11	37	theme	clostridial	1800:1810	arg1	members					1824:1830	clostridial cluster III members	1800:1830	clostridial cluster III members	1800:1830	In combination with the genera Anaerobacterium, Cellulosibacter, Ercella, Fastidiosipila, Mageeibacillus, Pseudobacteroides, Petroclostridium and Saccharofermentans, clostridial cluster III members formed a monophyletic clade within the order Clostridiales but that was clearly distinguished from other Ruminococcaceae members, which is proposed as a novel family, Hungateiclostridiaceae fam.
30124399	10	38	theme	gen.	1610:1613	arg1	nov.					1615:1618	Pseudoclostridium gen. nov.	1592:1618	Pseudoclostridium gen. nov.	1592:1618	Furthermore, analysis through 16S rRNA gene, ribosomal protein and whole genome sequences indicated that clostridial cluster III members should be reclassified into four novel genera for which the names Hungateiclostridium gen. nov., Thermoclostridium gen. nov., Ruminiclostridium gen. nov. and Pseudoclostridium gen. nov. are proposed.
30124399	3	39	theme	China	371:375	arg1	oily-sludge					338:348	oily-sludge	338:348	oily-sludge of Shengli oilfield, China	338:375	A rod-shaped, Gram-stain-positive, obligately anaerobic, xylan-degrading bacterium, SK-Y3T, was isolated from oily-sludge of Shengli oilfield, China.
30124399	8	40	theme	xylanilyticum	1263:1275	arg1	nov.					1282:1285	Petroclostridium xylanilyticum gen. nov.	1246:1285	Petroclostridium xylanilyticum gen. nov.	1246:1285	Based on distinct physiological and phylogenetic differences from the aforementioned described taxa, strain SK-Y3T (=DSM 103557T=ACCC 19952T) is proposed as the type strain of a novel species of a new genus, Petroclostridium xylanilyticum gen. nov., sp.
30124399	8	40	theme	xylanilyticum	1263:1275	arg1	genus					1239:1243	a new genus	1233:1243	a new genus	1233:1243	Based on distinct physiological and phylogenetic differences from the aforementioned described taxa, strain SK-Y3T (=DSM 103557T=ACCC 19952T) is proposed as the type strain of a novel species of a new genus, Petroclostridium xylanilyticum gen. nov., sp.
30124399	8	40	theme	xylanilyticum	1263:1275	arg1	sp					1288:1289	sp	1288:1289	sp	1288:1289	Based on distinct physiological and phylogenetic differences from the aforementioned described taxa, strain SK-Y3T (=DSM 103557T=ACCC 19952T) is proposed as the type strain of a novel species of a new genus, Petroclostridium xylanilyticum gen. nov., sp.
30124399	11	41	dep	genera	1658:1663	arg1	Petroclostridium					1759:1774	Petroclostridium	1759:1774	Petroclostridium	1759:1774	In combination with the genera Anaerobacterium, Cellulosibacter, Ercella, Fastidiosipila, Mageeibacillus, Pseudobacteroides, Petroclostridium and Saccharofermentans, clostridial cluster III members formed a monophyletic clade within the order Clostridiales but that was clearly distinguished from other Ruminococcaceae members, which is proposed as a novel family, Hungateiclostridiaceae fam.
30124399	11	41	dep	genera	1658:1663	arg1	Pseudobacteroides					1740:1756	Pseudobacteroides	1740:1756	Pseudobacteroides	1740:1756	In combination with the genera Anaerobacterium, Cellulosibacter, Ercella, Fastidiosipila, Mageeibacillus, Pseudobacteroides, Petroclostridium and Saccharofermentans, clostridial cluster III members formed a monophyletic clade within the order Clostridiales but that was clearly distinguished from other Ruminococcaceae members, which is proposed as a novel family, Hungateiclostridiaceae fam.
30124399	11	41	dep	genera	1658:1663	arg1	Cellulosibacter					1682:1696	Cellulosibacter	1682:1696	Cellulosibacter	1682:1696	In combination with the genera Anaerobacterium, Cellulosibacter, Ercella, Fastidiosipila, Mageeibacillus, Pseudobacteroides, Petroclostridium and Saccharofermentans, clostridial cluster III members formed a monophyletic clade within the order Clostridiales but that was clearly distinguished from other Ruminococcaceae members, which is proposed as a novel family, Hungateiclostridiaceae fam.
30124399	11	41	dep	genera	1658:1663	arg1	Fastidiosipila					1708:1721	Fastidiosipila	1708:1721	Fastidiosipila	1708:1721	In combination with the genera Anaerobacterium, Cellulosibacter, Ercella, Fastidiosipila, Mageeibacillus, Pseudobacteroides, Petroclostridium and Saccharofermentans, clostridial cluster III members formed a monophyletic clade within the order Clostridiales but that was clearly distinguished from other Ruminococcaceae members, which is proposed as a novel family, Hungateiclostridiaceae fam.
30124399	11	41	dep	genera	1658:1663	arg1	Saccharofermentans					1780:1797	Saccharofermentans	1780:1797	Saccharofermentans	1780:1797	In combination with the genera Anaerobacterium, Cellulosibacter, Ercella, Fastidiosipila, Mageeibacillus, Pseudobacteroides, Petroclostridium and Saccharofermentans, clostridial cluster III members formed a monophyletic clade within the order Clostridiales but that was clearly distinguished from other Ruminococcaceae members, which is proposed as a novel family, Hungateiclostridiaceae fam.
30124399	10	42	theme	Pseudoclostridium	1592:1608	arg1	nov.					1615:1618	Pseudoclostridium gen. nov.	1592:1618	Pseudoclostridium gen. nov.	1592:1618	Furthermore, analysis through 16S rRNA gene, ribosomal protein and whole genome sequences indicated that clostridial cluster III members should be reclassified into four novel genera for which the names Hungateiclostridium gen. nov., Thermoclostridium gen. nov., Ruminiclostridium gen. nov. and Pseudoclostridium gen. nov. are proposed.
30124399	11	43	with	combination	1637:1647	arg1	genera					1658:1663	the genera Anaerobacterium, Cellulosibacter, Ercella, Fastidiosipila, Mageeibacillus, Pseudobacteroides, Petroclostridium and Saccharofermentans	1654:1797	the genera Anaerobacterium, Cellulosibacter, Ercella, Fastidiosipila, Mageeibacillus, Pseudobacteroides, Petroclostridium and Saccharofermentans	1654:1797	In combination with the genera Anaerobacterium, Cellulosibacter, Ercella, Fastidiosipila, Mageeibacillus, Pseudobacteroides, Petroclostridium and Saccharofermentans, clostridial cluster III members formed a monophyletic clade within the order Clostridiales but that was clearly distinguished from other Ruminococcaceae members, which is proposed as a novel family, Hungateiclostridiaceae fam.
30124399	8	44	theme	type	1199:1202	arg1	SK-Y3T					1146:1151	strain SK-Y3T	1139:1151	strain SK-Y3T (=DSM 103557T=ACCC 19952T)	1139:1178	Based on distinct physiological and phylogenetic differences from the aforementioned described taxa, strain SK-Y3T (=DSM 103557T=ACCC 19952T) is proposed as the type strain of a novel species of a new genus, Petroclostridium xylanilyticum gen. nov., sp.
30124399	8	44	theme	type	1199:1202	arg1	strain					1204:1209	the type strain	1195:1209	the type strain of a novel species of a new genus, Petroclostridium xylanilyticum gen. nov., sp	1195:1289	Based on distinct physiological and phylogenetic differences from the aforementioned described taxa, strain SK-Y3T (=DSM 103557T=ACCC 19952T) is proposed as the type strain of a novel species of a new genus, Petroclostridium xylanilyticum gen. nov., sp.
30124399	5	45	theme	predominant	456:466	arg1	iso-C15 					511:518	iso-C15 	511:518	iso-C15 	511:518	The predominant cellular fatty acids of strain SK-Y3T were iso-C15 : 0, anteiso-C15 : 0 and iso-C17 : 0, and the main polar lipids were glycolipids (GL), lipids (L), phosphatidylglycerol (PG) and diphosphatidylglycerol (DPG); no respiratory quinones were detected.
30124399	5	45	theme	predominant	456:466	arg1	acids					483:487	The predominant cellular fatty acids	452:487	The predominant cellular fatty acids of strain SK-Y3T	452:504	The predominant cellular fatty acids of strain SK-Y3T were iso-C15 : 0, anteiso-C15 : 0 and iso-C17 : 0, and the main polar lipids were glycolipids (GL), lipids (L), phosphatidylglycerol (PG) and diphosphatidylglycerol (DPG); no respiratory quinones were detected.
30124399	1	46	theme	new	191:193	arg1	fam					218:220	a new Hungateiclostridiaceae fam	189:220	a new Hungateiclostridiaceae fam	189:220	nov., a xylan-degrading bacterium isolated from an oilfield, and reclassification of clostridial cluster III members into four novel genera in a new Hungateiclostridiaceae fam.
30124399	3	47	dep	rod-shaped	230:239	arg1	Gram-stain-positive					242:260	Gram-stain-positive	242:260	Gram-stain-positive	242:260	A rod-shaped, Gram-stain-positive, obligately anaerobic, xylan-degrading bacterium, SK-Y3T, was isolated from oily-sludge of Shengli oilfield, China.
30124399	8	48	theme	strain	1139:1144	arg1	SK-Y3T					1146:1151	strain SK-Y3T	1139:1151	strain SK-Y3T (=DSM 103557T=ACCC 19952T)	1139:1178	Based on distinct physiological and phylogenetic differences from the aforementioned described taxa, strain SK-Y3T (=DSM 103557T=ACCC 19952T) is proposed as the type strain of a novel species of a new genus, Petroclostridium xylanilyticum gen. nov., sp.
30124399	8	48	theme	strain	1139:1144	arg1	strain					1204:1209	the type strain	1195:1209	the type strain of a novel species of a new genus, Petroclostridium xylanilyticum gen. nov., sp	1195:1289	Based on distinct physiological and phylogenetic differences from the aforementioned described taxa, strain SK-Y3T (=DSM 103557T=ACCC 19952T) is proposed as the type strain of a novel species of a new genus, Petroclostridium xylanilyticum gen. nov., sp.
30124399	8	48	theme	strain	1139:1144	arg1	19952T					1172:1177	=DSM 103557T=ACCC 19952T	1154:1177	=DSM 103557T=ACCC 19952T	1154:1177	Based on distinct physiological and phylogenetic differences from the aforementioned described taxa, strain SK-Y3T (=DSM 103557T=ACCC 19952T) is proposed as the type strain of a novel species of a new genus, Petroclostridium xylanilyticum gen. nov., sp.
30124399	6	49	theme	37.2 mol	749:756	arg1	content					737:743	The genomic DNA G+C content	717:743	The genomic DNA G+C content	717:743	The genomic DNA G+C content was 37.2 mol%.
30124399	6	49	theme	37.2 mol	749:756	arg1	%					757:757	37.2 mol%	749:757	37.2 mol%	749:757	The genomic DNA G+C content was 37.2 mol%.
30124399	11	50	theme	other	1931:1935	arg1	members					1953:1959	other Ruminococcaceae members	1931:1959	other Ruminococcaceae members	1931:1959	In combination with the genera Anaerobacterium, Cellulosibacter, Ercella, Fastidiosipila, Mageeibacillus, Pseudobacteroides, Petroclostridium and Saccharofermentans, clostridial cluster III members formed a monophyletic clade within the order Clostridiales but that was clearly distinguished from other Ruminococcaceae members, which is proposed as a novel family, Hungateiclostridiaceae fam.
30124399	11	50	theme	other	1931:1935	arg1	family					1991:1996	a novel family	1983:1996	a novel family	1983:1996	In combination with the genera Anaerobacterium, Cellulosibacter, Ercella, Fastidiosipila, Mageeibacillus, Pseudobacteroides, Petroclostridium and Saccharofermentans, clostridial cluster III members formed a monophyletic clade within the order Clostridiales but that was clearly distinguished from other Ruminococcaceae members, which is proposed as a novel family, Hungateiclostridiaceae fam.
30124399	1	51	theme	Hungateiclostridiaceae	195:216	arg1	fam					218:220	a new Hungateiclostridiaceae fam	189:220	a new Hungateiclostridiaceae fam	189:220	nov., a xylan-degrading bacterium isolated from an oilfield, and reclassification of clostridial cluster III members into four novel genera in a new Hungateiclostridiaceae fam.
30124399	5	52	theme	respiratory	681:691	arg1	quinones					693:700	no respiratory quinones	678:700	no respiratory quinones	678:700	The predominant cellular fatty acids of strain SK-Y3T were iso-C15 : 0, anteiso-C15 : 0 and iso-C17 : 0, and the main polar lipids were glycolipids (GL), lipids (L), phosphatidylglycerol (PG) and diphosphatidylglycerol (DPG); no respiratory quinones were detected.
30124399	1	53	attach	isolated	80:87	arg2	nov.					46:49	nov.	46:49	nov.	46:49	nov., a xylan-degrading bacterium isolated from an oilfield, and reclassification of clostridial cluster III members into four novel genera in a new Hungateiclostridiaceae fam.
30124399	1	53	attach	isolated	80:87	arg2	bacterium					70:78	a xylan-degrading bacterium	52:78	a xylan-degrading bacterium isolated from an oilfield	52:104	nov., a xylan-degrading bacterium isolated from an oilfield, and reclassification of clostridial cluster III members into four novel genera in a new Hungateiclostridiaceae fam.
30124399	1	53	attach	isolated	80:87	arg1	oilfield					97:104	an oilfield	94:104	an oilfield	94:104	nov., a xylan-degrading bacterium isolated from an oilfield, and reclassification of clostridial cluster III members into four novel genera in a new Hungateiclostridiaceae fam.
30124399	7	54	theme	related	929:935	arg1	straminisolvens					990:1004	Clostridium straminisolvens	978:1004	Clostridium straminisolvens	978:1004	Phylogenetic analysis of 16S rRNA gene sequences showed that strain SK-Y3T belongs to clostridial cluster III, exhibiting 91-92% sequence similarity to the most closely related species, namely Clostridium clariflavum, Clostridium straminisolvens and Acetivibrio cellulolyticus.
30124399	7	54	theme	related	929:935	arg1	species					937:943	the most closely related species	912:943	the most closely related species	912:943	Phylogenetic analysis of 16S rRNA gene sequences showed that strain SK-Y3T belongs to clostridial cluster III, exhibiting 91-92% sequence similarity to the most closely related species, namely Clostridium clariflavum, Clostridium straminisolvens and Acetivibrio cellulolyticus.
30124399	7	54	theme	related	929:935	arg1	Acetivibrio					1010:1020	Acetivibrio	1010:1020	Acetivibrio	1010:1020	Phylogenetic analysis of 16S rRNA gene sequences showed that strain SK-Y3T belongs to clostridial cluster III, exhibiting 91-92% sequence similarity to the most closely related species, namely Clostridium clariflavum, Clostridium straminisolvens and Acetivibrio cellulolyticus.
30124399	7	54	theme	related	929:935	arg1	clariflavum					965:975	Clostridium clariflavum	953:975	Clostridium clariflavum	953:975	Phylogenetic analysis of 16S rRNA gene sequences showed that strain SK-Y3T belongs to clostridial cluster III, exhibiting 91-92% sequence similarity to the most closely related species, namely Clostridium clariflavum, Clostridium straminisolvens and Acetivibrio cellulolyticus.
30124399	0	55	dep	sp	42:43	arg1	nov.					36:39	Petroclostridium xylanilyticum gen. nov.	0:39	Petroclostridium xylanilyticum gen. nov.	0:39	Petroclostridium xylanilyticum gen. nov., sp.
30124399	11	56	theme	cluster	1812:1818	arg1	members					1824:1830	clostridial cluster III members	1800:1830	clostridial cluster III members	1800:1830	In combination with the genera Anaerobacterium, Cellulosibacter, Ercella, Fastidiosipila, Mageeibacillus, Pseudobacteroides, Petroclostridium and Saccharofermentans, clostridial cluster III members formed a monophyletic clade within the order Clostridiales but that was clearly distinguished from other Ruminococcaceae members, which is proposed as a novel family, Hungateiclostridiaceae fam.
30124399	11	57	theme	monophyletic	1841:1852	arg1	clade					1854:1858	a monophyletic clade	1839:1858	a monophyletic clade within the order Clostridiales but that was clearly distinguished from other Ruminococcaceae members, which is proposed as a novel family, Hungateiclostridiaceae fam	1839:2024	In combination with the genera Anaerobacterium, Cellulosibacter, Ercella, Fastidiosipila, Mageeibacillus, Pseudobacteroides, Petroclostridium and Saccharofermentans, clostridial cluster III members formed a monophyletic clade within the order Clostridiales but that was clearly distinguished from other Ruminococcaceae members, which is proposed as a novel family, Hungateiclostridiaceae fam.
30124399	10	58	theme	clostridial	1402:1412	arg1	members					1426:1432	clostridial cluster III members	1402:1432	clostridial cluster III members	1402:1432	Furthermore, analysis through 16S rRNA gene, ribosomal protein and whole genome sequences indicated that clostridial cluster III members should be reclassified into four novel genera for which the names Hungateiclostridium gen. nov., Thermoclostridium gen. nov., Ruminiclostridium gen. nov. and Pseudoclostridium gen. nov. are proposed.
30124399	10	59	theme	whole	1364:1368	arg1	sequences					1377:1385	whole genome sequences	1364:1385	whole genome sequences	1364:1385	Furthermore, analysis through 16S rRNA gene, ribosomal protein and whole genome sequences indicated that clostridial cluster III members should be reclassified into four novel genera for which the names Hungateiclostridium gen. nov., Thermoclostridium gen. nov., Ruminiclostridium gen. nov. and Pseudoclostridium gen. nov. are proposed.
30124399	7	60	theme	sequence	889:896	arg1	similarity					898:907	91-92% sequence similarity	882:907	91-92% sequence similarity to the most closely related species, namely Clostridium clariflavum, Clostridium straminisolvens and Acetivibrio cellulolyticus	882:1035	Phylogenetic analysis of 16S rRNA gene sequences showed that strain SK-Y3T belongs to clostridial cluster III, exhibiting 91-92% sequence similarity to the most closely related species, namely Clostridium clariflavum, Clostridium straminisolvens and Acetivibrio cellulolyticus.
30124399	7	61	theme	sequences	799:807	arg1	analysis					773:780	Phylogenetic analysis	760:780	Phylogenetic analysis of 16S rRNA gene sequences	760:807	Phylogenetic analysis of 16S rRNA gene sequences showed that strain SK-Y3T belongs to clostridial cluster III, exhibiting 91-92% sequence similarity to the most closely related species, namely Clostridium clariflavum, Clostridium straminisolvens and Acetivibrio cellulolyticus.
30124399	3	62	theme	xylan-degrading	285:299	arg1	bacterium					301:309	A rod-shaped, Gram-stain-positive, obligately anaerobic, xylan-degrading bacterium	228:309	bacterium	301:309	A rod-shaped, Gram-stain-positive, obligately anaerobic, xylan-degrading bacterium, SK-Y3T, was isolated from oily-sludge of Shengli oilfield, China.
30124399	3	62	theme	xylan-degrading	285:299	arg1	SK-Y3T					312:317	SK-Y3T	312:317	SK-Y3T	312:317	A rod-shaped, Gram-stain-positive, obligately anaerobic, xylan-degrading bacterium, SK-Y3T, was isolated from oily-sludge of Shengli oilfield, China.
30124399	6	63	theme	G+C	733:735	arg1	content					737:743	The genomic DNA G+C content	717:743	The genomic DNA G+C content	717:743	The genomic DNA G+C content was 37.2 mol%.
30124399	6	63	theme	G+C	733:735	arg1	%					757:757	37.2 mol%	749:757	37.2 mol%	749:757	The genomic DNA G+C content was 37.2 mol%.
30124399	8	64	theme	novel	1216:1220	arg1	species					1222:1228	a novel species	1214:1228	a novel species of a new genus, Petroclostridium xylanilyticum gen. nov., sp	1214:1289	Based on distinct physiological and phylogenetic differences from the aforementioned described taxa, strain SK-Y3T (=DSM 103557T=ACCC 19952T) is proposed as the type strain of a novel species of a new genus, Petroclostridium xylanilyticum gen. nov., sp.
30124399	11	65	theme	Hungateiclostridiaceae	1999:2020	arg1	family					1991:1996	a novel family	1983:1996	a novel family	1983:1996	In combination with the genera Anaerobacterium, Cellulosibacter, Ercella, Fastidiosipila, Mageeibacillus, Pseudobacteroides, Petroclostridium and Saccharofermentans, clostridial cluster III members formed a monophyletic clade within the order Clostridiales but that was clearly distinguished from other Ruminococcaceae members, which is proposed as a novel family, Hungateiclostridiaceae fam.
30124399	11	65	theme	Hungateiclostridiaceae	1999:2020	arg1	fam					2022:2024	Hungateiclostridiaceae fam	1999:2024	Hungateiclostridiaceae fam	1999:2024	In combination with the genera Anaerobacterium, Cellulosibacter, Ercella, Fastidiosipila, Mageeibacillus, Pseudobacteroides, Petroclostridium and Saccharofermentans, clostridial cluster III members formed a monophyletic clade within the order Clostridiales but that was clearly distinguished from other Ruminococcaceae members, which is proposed as a novel family, Hungateiclostridiaceae fam.
30124399	8	66	theme	Petroclostridium	1246:1261	arg1	nov.					1282:1285	Petroclostridium xylanilyticum gen. nov.	1246:1285	Petroclostridium xylanilyticum gen. nov.	1246:1285	Based on distinct physiological and phylogenetic differences from the aforementioned described taxa, strain SK-Y3T (=DSM 103557T=ACCC 19952T) is proposed as the type strain of a novel species of a new genus, Petroclostridium xylanilyticum gen. nov., sp.
30124399	8	66	theme	Petroclostridium	1246:1261	arg1	genus					1239:1243	a new genus	1233:1243	a new genus	1233:1243	Based on distinct physiological and phylogenetic differences from the aforementioned described taxa, strain SK-Y3T (=DSM 103557T=ACCC 19952T) is proposed as the type strain of a novel species of a new genus, Petroclostridium xylanilyticum gen. nov., sp.
30124399	8	66	theme	Petroclostridium	1246:1261	arg1	sp					1288:1289	sp	1288:1289	sp	1288:1289	Based on distinct physiological and phylogenetic differences from the aforementioned described taxa, strain SK-Y3T (=DSM 103557T=ACCC 19952T) is proposed as the type strain of a novel species of a new genus, Petroclostridium xylanilyticum gen. nov., sp.
30124399	6	67	theme	DNA	729:731	arg1	content					737:743	The genomic DNA G+C content	717:743	The genomic DNA G+C content	717:743	The genomic DNA G+C content was 37.2 mol%.
30124399	6	67	theme	DNA	729:731	arg1	%					757:757	37.2 mol%	749:757	37.2 mol%	749:757	The genomic DNA G+C content was 37.2 mol%.
30124399	8	68	theme	aforementioned	1108:1121	arg1	taxa					1133:1136	the aforementioned described taxa	1104:1136	the aforementioned described taxa	1104:1136	Based on distinct physiological and phylogenetic differences from the aforementioned described taxa, strain SK-Y3T (=DSM 103557T=ACCC 19952T) is proposed as the type strain of a novel species of a new genus, Petroclostridium xylanilyticum gen. nov., sp.
30124399	5	69	theme	strain	492:497	arg1	SK-Y3T					499:504	strain SK-Y3T	492:504	strain SK-Y3T	492:504	The predominant cellular fatty acids of strain SK-Y3T were iso-C15 : 0, anteiso-C15 : 0 and iso-C17 : 0, and the main polar lipids were glycolipids (GL), lipids (L), phosphatidylglycerol (PG) and diphosphatidylglycerol (DPG); no respiratory quinones were detected.
30124399	8	70	theme	species	1222:1228	arg1	SK-Y3T					1146:1151	strain SK-Y3T	1139:1151	strain SK-Y3T (=DSM 103557T=ACCC 19952T)	1139:1178	Based on distinct physiological and phylogenetic differences from the aforementioned described taxa, strain SK-Y3T (=DSM 103557T=ACCC 19952T) is proposed as the type strain of a novel species of a new genus, Petroclostridium xylanilyticum gen. nov., sp.
30124399	8	70	theme	species	1222:1228	arg1	strain					1204:1209	the type strain	1195:1209	the type strain of a novel species of a new genus, Petroclostridium xylanilyticum gen. nov., sp	1195:1289	Based on distinct physiological and phylogenetic differences from the aforementioned described taxa, strain SK-Y3T (=DSM 103557T=ACCC 19952T) is proposed as the type strain of a novel species of a new genus, Petroclostridium xylanilyticum gen. nov., sp.
30124399	7	71	theme	rRNA	789:792	arg1	sequences					799:807	16S rRNA gene sequences	785:807	16S rRNA gene sequences	785:807	Phylogenetic analysis of 16S rRNA gene sequences showed that strain SK-Y3T belongs to clostridial cluster III, exhibiting 91-92% sequence similarity to the most closely related species, namely Clostridium clariflavum, Clostridium straminisolvens and Acetivibrio cellulolyticus.
30124399	8	72	theme	distinct	1047:1054	arg1	differences					1087:1097	distinct physiological and phylogenetic differences	1047:1097	distinct physiological and phylogenetic differences from the aforementioned described taxa	1047:1136	Based on distinct physiological and phylogenetic differences from the aforementioned described taxa, strain SK-Y3T (=DSM 103557T=ACCC 19952T) is proposed as the type strain of a novel species of a new genus, Petroclostridium xylanilyticum gen. nov., sp.
30124399	6	73	theme	genomic	721:727	arg1	content					737:743	The genomic DNA G+C content	717:743	The genomic DNA G+C content	717:743	The genomic DNA G+C content was 37.2 mol%.
30124399	6	73	theme	genomic	721:727	arg1	%					757:757	37.2 mol%	749:757	37.2 mol%	749:757	The genomic DNA G+C content was 37.2 mol%.
30124399	11	74	theme	Ruminococcaceae	1937:1951	arg1	members					1953:1959	other Ruminococcaceae members	1931:1959	other Ruminococcaceae members	1931:1959	In combination with the genera Anaerobacterium, Cellulosibacter, Ercella, Fastidiosipila, Mageeibacillus, Pseudobacteroides, Petroclostridium and Saccharofermentans, clostridial cluster III members formed a monophyletic clade within the order Clostridiales but that was clearly distinguished from other Ruminococcaceae members, which is proposed as a novel family, Hungateiclostridiaceae fam.
30124399	11	74	theme	Ruminococcaceae	1937:1951	arg1	family					1991:1996	a novel family	1983:1996	a novel family	1983:1996	In combination with the genera Anaerobacterium, Cellulosibacter, Ercella, Fastidiosipila, Mageeibacillus, Pseudobacteroides, Petroclostridium and Saccharofermentans, clostridial cluster III members formed a monophyletic clade within the order Clostridiales but that was clearly distinguished from other Ruminococcaceae members, which is proposed as a novel family, Hungateiclostridiaceae fam.
30124399	1	75	from	genera	179:184	arg1	fam					218:220	a new Hungateiclostridiaceae fam	189:220	a new Hungateiclostridiaceae fam	189:220	nov., a xylan-degrading bacterium isolated from an oilfield, and reclassification of clostridial cluster III members into four novel genera in a new Hungateiclostridiaceae fam.
30124399	1	76	theme	clostridial	131:141	arg1	members					155:161	clostridial cluster III members	131:161	clostridial cluster III members	131:161	nov., a xylan-degrading bacterium isolated from an oilfield, and reclassification of clostridial cluster III members into four novel genera in a new Hungateiclostridiaceae fam.
30124399	7	77	theme	gene	794:797	arg1	sequences					799:807	16S rRNA gene sequences	785:807	16S rRNA gene sequences	785:807	Phylogenetic analysis of 16S rRNA gene sequences showed that strain SK-Y3T belongs to clostridial cluster III, exhibiting 91-92% sequence similarity to the most closely related species, namely Clostridium clariflavum, Clostridium straminisolvens and Acetivibrio cellulolyticus.
30124399	8	78	theme	=DSM	1154:1157	arg1	SK-Y3T					1146:1151	strain SK-Y3T	1139:1151	strain SK-Y3T (=DSM 103557T=ACCC 19952T)	1139:1178	Based on distinct physiological and phylogenetic differences from the aforementioned described taxa, strain SK-Y3T (=DSM 103557T=ACCC 19952T) is proposed as the type strain of a novel species of a new genus, Petroclostridium xylanilyticum gen. nov., sp.
30124399	8	78	theme	=DSM	1154:1157	arg1	19952T					1172:1177	=DSM 103557T=ACCC 19952T	1154:1177	=DSM 103557T=ACCC 19952T	1154:1177	Based on distinct physiological and phylogenetic differences from the aforementioned described taxa, strain SK-Y3T (=DSM 103557T=ACCC 19952T) is proposed as the type strain of a novel species of a new genus, Petroclostridium xylanilyticum gen. nov., sp.
30124399	0	79	theme	gen.	31:34	arg1	nov.					36:39	Petroclostridium xylanilyticum gen. nov.	0:39	Petroclostridium xylanilyticum gen. nov.	0:39	Petroclostridium xylanilyticum gen. nov., sp.
30124399	10	80	dep	names	1494:1498	arg1	Hungateiclostridium					1500:1518	Hungateiclostridium	1500:1518	Hungateiclostridium	1500:1518	Furthermore, analysis through 16S rRNA gene, ribosomal protein and whole genome sequences indicated that clostridial cluster III members should be reclassified into four novel genera for which the names Hungateiclostridium gen. nov., Thermoclostridium gen. nov., Ruminiclostridium gen. nov. and Pseudoclostridium gen. nov. are proposed.
30124399	10	80	dep	names	1494:1498	arg1	nov.					1554:1557	nov.	1554:1557	nov.	1554:1557	Furthermore, analysis through 16S rRNA gene, ribosomal protein and whole genome sequences indicated that clostridial cluster III members should be reclassified into four novel genera for which the names Hungateiclostridium gen. nov., Thermoclostridium gen. nov., Ruminiclostridium gen. nov. and Pseudoclostridium gen. nov. are proposed.
30124399	10	80	dep	names	1494:1498	arg1	gen.					1520:1523	gen.	1520:1523	gen.	1520:1523	Furthermore, analysis through 16S rRNA gene, ribosomal protein and whole genome sequences indicated that clostridial cluster III members should be reclassified into four novel genera for which the names Hungateiclostridium gen. nov., Thermoclostridium gen. nov., Ruminiclostridium gen. nov. and Pseudoclostridium gen. nov. are proposed.
30124399	10	80	dep	names	1494:1498	arg1	Ruminiclostridium					1560:1576	Ruminiclostridium	1560:1576	Ruminiclostridium	1560:1576	Furthermore, analysis through 16S rRNA gene, ribosomal protein and whole genome sequences indicated that clostridial cluster III members should be reclassified into four novel genera for which the names Hungateiclostridium gen. nov., Thermoclostridium gen. nov., Ruminiclostridium gen. nov. and Pseudoclostridium gen. nov. are proposed.
30124399	10	80	dep	names	1494:1498	arg1	gen.					1578:1581	gen.	1578:1581	gen.	1578:1581	Furthermore, analysis through 16S rRNA gene, ribosomal protein and whole genome sequences indicated that clostridial cluster III members should be reclassified into four novel genera for which the names Hungateiclostridium gen. nov., Thermoclostridium gen. nov., Ruminiclostridium gen. nov. and Pseudoclostridium gen. nov. are proposed.
30124399	10	80	dep	names	1494:1498	arg1	nov.					1525:1528	nov.	1525:1528	nov.	1525:1528	Furthermore, analysis through 16S rRNA gene, ribosomal protein and whole genome sequences indicated that clostridial cluster III members should be reclassified into four novel genera for which the names Hungateiclostridium gen. nov., Thermoclostridium gen. nov., Ruminiclostridium gen. nov. and Pseudoclostridium gen. nov. are proposed.
30124399	10	80	dep	names	1494:1498	arg1	Thermoclostridium					1531:1547	Thermoclostridium	1531:1547	Thermoclostridium	1531:1547	Furthermore, analysis through 16S rRNA gene, ribosomal protein and whole genome sequences indicated that clostridial cluster III members should be reclassified into four novel genera for which the names Hungateiclostridium gen. nov., Thermoclostridium gen. nov., Ruminiclostridium gen. nov. and Pseudoclostridium gen. nov. are proposed.
30124399	10	80	dep	names	1494:1498	arg1	nov.					1583:1586	nov.	1583:1586	nov.	1583:1586	Furthermore, analysis through 16S rRNA gene, ribosomal protein and whole genome sequences indicated that clostridial cluster III members should be reclassified into four novel genera for which the names Hungateiclostridium gen. nov., Thermoclostridium gen. nov., Ruminiclostridium gen. nov. and Pseudoclostridium gen. nov. are proposed.
30124399	10	80	dep	names	1494:1498	arg1	gen.					1549:1552	gen.	1549:1552	gen.	1549:1552	Furthermore, analysis through 16S rRNA gene, ribosomal protein and whole genome sequences indicated that clostridial cluster III members should be reclassified into four novel genera for which the names Hungateiclostridium gen. nov., Thermoclostridium gen. nov., Ruminiclostridium gen. nov. and Pseudoclostridium gen. nov. are proposed.
30124399	1	81	theme	cluster	143:149	arg1	members					155:161	clostridial cluster III members	131:161	clostridial cluster III members	131:161	nov., a xylan-degrading bacterium isolated from an oilfield, and reclassification of clostridial cluster III members into four novel genera in a new Hungateiclostridiaceae fam.
30124399	8	82	theme	phylogenetic	1074:1085	arg1	differences					1087:1097	distinct physiological and phylogenetic differences	1047:1097	distinct physiological and phylogenetic differences from the aforementioned described taxa	1047:1136	Based on distinct physiological and phylogenetic differences from the aforementioned described taxa, strain SK-Y3T (=DSM 103557T=ACCC 19952T) is proposed as the type strain of a novel species of a new genus, Petroclostridium xylanilyticum gen. nov., sp.
31259325	9	0	theme	reduction	1615:1623	arg1	approach					1625:1632	this salt reduction approach	1605:1632	this salt reduction approach for a real food	1605:1648	Consumer tests on a tomato soup, to validate this salt reduction approach for a real food, revealed a possible 25% salt reduction, compared to current UK products.
31259325	9	1	dep	tests	1569:1573	arg1	validate					1596:1603	validate	1596:1603	to validate this salt reduction approach for a real food	1593:1648	Consumer tests on a tomato soup, to validate this salt reduction approach for a real food, revealed a possible 25% salt reduction, compared to current UK products.
31259325	2	2	theme	Salt	358:361	arg1	levels					363:368	Salt levels	358:368	Salt levels	358:368	Salt levels were 1.5 g salt and 0.47 g salt per 100 g external and internal aqueous phases, respectively.
31259325	6	3	theme	PGPR	1199:1202	arg1	content					1204:1210	the same PGPR content	1190:1210	the same PGPR content	1190:1210	One day after processing, encapsulation efficiency (EE) was as predicted from the composition for OSA emulsions, while at the same PGPR content an external aqueous phase was incorporated into the oil droplets of the WRS emulsion increasing EE.
31259325	5	4	theme	lower	967:971	arg1	temperature					988:998	lower level PGPR low temperature	967:998	lower level PGPR low temperature	967:998	Emulsions were stable for three months, except OSA and lower level PGPR low temperature processed WRS emulsions lost salt into the external emulsion phase.
31259325	4	5	theme	g	873:873	arg1	PGPR					875:878	0.57 g PGPR	868:878	0.57 g PGPR per 100 g emulsion	868:897	OSA starch was used under previously applied conditions (2, 3, 4 g starch, 0.57 g PGPR per 100 g emulsion, 25 ± 5 °C).
31259325	4	5	theme	g	873:873	arg1	g					858:858	2, 3, 4 g	850:858	2, 3, 4 g starch	850:865	OSA starch was used under previously applied conditions (2, 3, 4 g starch, 0.57 g PGPR per 100 g emulsion, 25 ± 5 °C).
31259325	6	6	theme	WRS	1284:1286	arg1	EE					1308:1309	the WRS emulsion increasing EE	1280:1309	the WRS emulsion increasing EE	1280:1309	One day after processing, encapsulation efficiency (EE) was as predicted from the composition for OSA emulsions, while at the same PGPR content an external aqueous phase was incorporated into the oil droplets of the WRS emulsion increasing EE.
31259325	5	7	theme	PGPR	979:982	arg1	temperature					988:998	lower level PGPR low temperature	967:998	lower level PGPR low temperature	967:998	Emulsions were stable for three months, except OSA and lower level PGPR low temperature processed WRS emulsions lost salt into the external emulsion phase.
31259325	9	8	from	tests	1569:1573	arg1	soup					1587:1590	a tomato soup	1578:1590	a tomato soup	1578:1590	Consumer tests on a tomato soup, to validate this salt reduction approach for a real food, revealed a possible 25% salt reduction, compared to current UK products.
31259325	9	9	theme	real	1640:1643	arg1	food					1645:1648	a real food	1638:1648	a real food	1638:1648	Consumer tests on a tomato soup, to validate this salt reduction approach for a real food, revealed a possible 25% salt reduction, compared to current UK products.
31259325	3	10	theme	lipophilic	609:618	arg1	emulsifier					620:629	a lipophilic emulsifier	607:629	a lipophilic emulsifier (0.29, 0.57 g per 100 g emulsion)	607:663	Variables were the starch content (2, 3, 4 g per 100 g emulsion; 20 g oil per 100 g emulsion), level of polyglycerol polyricinoleate (PGPR) as a lipophilic emulsifier (0.29, 0.57 g per 100 g emulsion) and ambient-pressure processing temperature for WRS gelatinisation, the non-chemical modification process, (75 ± 3, 88 ± 5 °C).
31259325	4	11	dep	g	858:858	arg1	starch					860:865	starch	860:865	2, 3, 4 g starch	850:865	OSA starch was used under previously applied conditions (2, 3, 4 g starch, 0.57 g PGPR per 100 g emulsion, 25 ± 5 °C).
31259325	8	12	theme	salt	1458:1461	arg1	approach					1473:1480	this salt reduction approach	1453:1480	this salt reduction approach	1453:1480	The results revealed that the efficacy of this salt reduction approach was enhanced for gelatinised WRS compared to OSA starch stabilised emulsions.
31259325	1	13	theme	rice	209:212	arg1	starch					214:219	non-chemically modified waxy rice starch	180:219	non-chemically modified waxy rice starch (WRS)	180:225	Water-in-oil-in-water emulsions containing an internalised salt solution were stabilised with non-chemically modified waxy rice starch (WRS), and octinyl succinic anhydride (OSA) as reference, to release salt during oral processing due to amylase-induced destabilisation.
31259325	1	13	theme	rice	209:212	arg1	WRS					222:224	WRS	222:224	WRS	222:224	Water-in-oil-in-water emulsions containing an internalised salt solution were stabilised with non-chemically modified waxy rice starch (WRS), and octinyl succinic anhydride (OSA) as reference, to release salt during oral processing due to amylase-induced destabilisation.
31259325	3	14	theme	g	517:517	arg1	oil					534:536	100 g emulsion; 20 g oil	513:536	100 g emulsion; 20 g oil	513:536	Variables were the starch content (2, 3, 4 g per 100 g emulsion; 20 g oil per 100 g emulsion), level of polyglycerol polyricinoleate (PGPR) as a lipophilic emulsifier (0.29, 0.57 g per 100 g emulsion) and ambient-pressure processing temperature for WRS gelatinisation, the non-chemical modification process, (75 ± 3, 88 ± 5 °C).
31259325	6	15	theme	oil	1264:1266	arg1	droplets					1268:1275	the oil droplets	1260:1275	the oil droplets of the WRS emulsion increasing EE	1260:1309	One day after processing, encapsulation efficiency (EE) was as predicted from the composition for OSA emulsions, while at the same PGPR content an external aqueous phase was incorporated into the oil droplets of the WRS emulsion increasing EE.
31259325	7	16	theme	paired	1384:1389	arg1	testing					1402:1408	paired comparison testing	1384:1408	paired comparison testing	1384:1408	Salt release was assessed in vitro and through sensory evaluation using paired comparison testing.
31259325	6	17	theme	encapsulation	1094:1106	arg1	EE					1120:1121	EE	1120:1121	EE	1120:1121	One day after processing, encapsulation efficiency (EE) was as predicted from the composition for OSA emulsions, while at the same PGPR content an external aqueous phase was incorporated into the oil droplets of the WRS emulsion increasing EE.
31259325	6	17	theme	encapsulation	1094:1106	arg1	efficiency					1108:1117	encapsulation efficiency	1094:1117	encapsulation efficiency (EE)	1094:1122	One day after processing, encapsulation efficiency (EE) was as predicted from the composition for OSA emulsions, while at the same PGPR content an external aqueous phase was incorporated into the oil droplets of the WRS emulsion increasing EE.
31259325	9	18	theme	25	1671:1672	arg1	%					1673:1673	%	1673:1673	%	1673:1673	Consumer tests on a tomato soup, to validate this salt reduction approach for a real food, revealed a possible 25% salt reduction, compared to current UK products.
31259325	9	19	theme	Consumer	1560:1567	arg1	tests					1569:1573	Consumer tests	1560:1573	Consumer tests	1560:1573	Consumer tests on a tomato soup, to validate this salt reduction approach for a real food, revealed a possible 25% salt reduction, compared to current UK products.
31259325	8	20	theme	gelatinised	1499:1509	arg1	WRS					1511:1513	gelatinised WRS	1499:1513	gelatinised WRS	1499:1513	The results revealed that the efficacy of this salt reduction approach was enhanced for gelatinised WRS compared to OSA starch stabilised emulsions.
31259325	9	21	theme	salt	1675:1678	arg1	reduction					1680:1688	a possible 25% salt reduction	1660:1688	a possible 25% salt reduction	1660:1688	Consumer tests on a tomato soup, to validate this salt reduction approach for a real food, revealed a possible 25% salt reduction, compared to current UK products.
31259325	3	22	dep	WRS	713:715	arg1	process					763:769	the non-chemical modification process	733:769	the non-chemical modification process	733:769	Variables were the starch content (2, 3, 4 g per 100 g emulsion; 20 g oil per 100 g emulsion), level of polyglycerol polyricinoleate (PGPR) as a lipophilic emulsifier (0.29, 0.57 g per 100 g emulsion) and ambient-pressure processing temperature for WRS gelatinisation, the non-chemical modification process, (75 ± 3, 88 ± 5 °C).
31259325	3	22	dep	WRS	713:715	arg1	gelatinisation					717:730	gelatinisation	717:730	gelatinisation	717:730	Variables were the starch content (2, 3, 4 g per 100 g emulsion; 20 g oil per 100 g emulsion), level of polyglycerol polyricinoleate (PGPR) as a lipophilic emulsifier (0.29, 0.57 g per 100 g emulsion) and ambient-pressure processing temperature for WRS gelatinisation, the non-chemical modification process, (75 ± 3, 88 ± 5 °C).
31259325	1	23	theme	salt	145:148	arg1	solution					150:157	an internalised salt solution	129:157	an internalised salt solution	129:157	Water-in-oil-in-water emulsions containing an internalised salt solution were stabilised with non-chemically modified waxy rice starch (WRS), and octinyl succinic anhydride (OSA) as reference, to release salt during oral processing due to amylase-induced destabilisation.
31259325	3	24	theme	modification	750:761	arg1	process					763:769	the non-chemical modification process	733:769	the non-chemical modification process	733:769	Variables were the starch content (2, 3, 4 g per 100 g emulsion; 20 g oil per 100 g emulsion), level of polyglycerol polyricinoleate (PGPR) as a lipophilic emulsifier (0.29, 0.57 g per 100 g emulsion) and ambient-pressure processing temperature for WRS gelatinisation, the non-chemical modification process, (75 ± 3, 88 ± 5 °C).
31259325	3	24	theme	modification	750:761	arg1	gelatinisation					717:730	gelatinisation	717:730	gelatinisation	717:730	Variables were the starch content (2, 3, 4 g per 100 g emulsion; 20 g oil per 100 g emulsion), level of polyglycerol polyricinoleate (PGPR) as a lipophilic emulsifier (0.29, 0.57 g per 100 g emulsion) and ambient-pressure processing temperature for WRS gelatinisation, the non-chemical modification process, (75 ± 3, 88 ± 5 °C).
31259325	5	25	theme	external	1043:1050	arg1	phase					1061:1065	the external emulsion phase	1039:1065	the external emulsion phase	1039:1065	Emulsions were stable for three months, except OSA and lower level PGPR low temperature processed WRS emulsions lost salt into the external emulsion phase.
31259325	3	26	theme	processing	686:695	arg1	temperature					697:707	ambient-pressure processing temperature	669:707	ambient-pressure processing temperature for WRS gelatinisation, the non-chemical modification process, (75 ± 3, 88 ± 5 °C)	669:790	Variables were the starch content (2, 3, 4 g per 100 g emulsion; 20 g oil per 100 g emulsion), level of polyglycerol polyricinoleate (PGPR) as a lipophilic emulsifier (0.29, 0.57 g per 100 g emulsion) and ambient-pressure processing temperature for WRS gelatinisation, the non-chemical modification process, (75 ± 3, 88 ± 5 °C).
31259325	3	27	dep	gelatinisation	717:730	arg1	°C					788:789	88 ± 5 °C	781:789	88 ± 5 °C	781:789	Variables were the starch content (2, 3, 4 g per 100 g emulsion; 20 g oil per 100 g emulsion), level of polyglycerol polyricinoleate (PGPR) as a lipophilic emulsifier (0.29, 0.57 g per 100 g emulsion) and ambient-pressure processing temperature for WRS gelatinisation, the non-chemical modification process, (75 ± 3, 88 ± 5 °C).
31259325	3	27	dep	gelatinisation	717:730	arg1	±					776:776	75 ± 3	773:778	75 ± 3	773:778	Variables were the starch content (2, 3, 4 g per 100 g emulsion; 20 g oil per 100 g emulsion), level of polyglycerol polyricinoleate (PGPR) as a lipophilic emulsifier (0.29, 0.57 g per 100 g emulsion) and ambient-pressure processing temperature for WRS gelatinisation, the non-chemical modification process, (75 ± 3, 88 ± 5 °C).
31259325	3	28	theme	g	546:546	arg1	emulsion					548:555	100 g emulsion	542:555	100 g emulsion	542:555	Variables were the starch content (2, 3, 4 g per 100 g emulsion; 20 g oil per 100 g emulsion), level of polyglycerol polyricinoleate (PGPR) as a lipophilic emulsifier (0.29, 0.57 g per 100 g emulsion) and ambient-pressure processing temperature for WRS gelatinisation, the non-chemical modification process, (75 ± 3, 88 ± 5 °C).
31259325	6	29	theme	same	1194:1197	arg1	content					1204:1210	the same PGPR content	1190:1210	the same PGPR content	1190:1210	One day after processing, encapsulation efficiency (EE) was as predicted from the composition for OSA emulsions, while at the same PGPR content an external aqueous phase was incorporated into the oil droplets of the WRS emulsion increasing EE.
31259325	6	30	theme	increasing	1297:1306	arg1	EE					1308:1309	the WRS emulsion increasing EE	1280:1309	the WRS emulsion increasing EE	1280:1309	One day after processing, encapsulation efficiency (EE) was as predicted from the composition for OSA emulsions, while at the same PGPR content an external aqueous phase was incorporated into the oil droplets of the WRS emulsion increasing EE.
31259325	9	31	theme	UK	1711:1712	arg1	products					1714:1721	current UK products	1703:1721	current UK products	1703:1721	Consumer tests on a tomato soup, to validate this salt reduction approach for a real food, revealed a possible 25% salt reduction, compared to current UK products.
31259325	1	32	theme	modified	195:202	arg1	starch					214:219	non-chemically modified waxy rice starch	180:219	non-chemically modified waxy rice starch (WRS)	180:225	Water-in-oil-in-water emulsions containing an internalised salt solution were stabilised with non-chemically modified waxy rice starch (WRS), and octinyl succinic anhydride (OSA) as reference, to release salt during oral processing due to amylase-induced destabilisation.
31259325	1	32	theme	modified	195:202	arg1	WRS					222:224	WRS	222:224	WRS	222:224	Water-in-oil-in-water emulsions containing an internalised salt solution were stabilised with non-chemically modified waxy rice starch (WRS), and octinyl succinic anhydride (OSA) as reference, to release salt during oral processing due to amylase-induced destabilisation.
31259325	3	33	theme	5	786:786	arg1	±					784:784	±	784:784	±	784:784	Variables were the starch content (2, 3, 4 g per 100 g emulsion; 20 g oil per 100 g emulsion), level of polyglycerol polyricinoleate (PGPR) as a lipophilic emulsifier (0.29, 0.57 g per 100 g emulsion) and ambient-pressure processing temperature for WRS gelatinisation, the non-chemical modification process, (75 ± 3, 88 ± 5 °C).
31259325	3	34	theme	88	781:782	arg1	±					784:784	±	784:784	±	784:784	Variables were the starch content (2, 3, 4 g per 100 g emulsion; 20 g oil per 100 g emulsion), level of polyglycerol polyricinoleate (PGPR) as a lipophilic emulsifier (0.29, 0.57 g per 100 g emulsion) and ambient-pressure processing temperature for WRS gelatinisation, the non-chemical modification process, (75 ± 3, 88 ± 5 °C).
31259325	2	35	theme	internal	425:432	arg1	phases					442:447	external and internal aqueous phases	412:447	external and internal aqueous phases	412:447	Salt levels were 1.5 g salt and 0.47 g salt per 100 g external and internal aqueous phases, respectively.
31259325	3	36	dep	content	490:496	arg1	g					507:507	2, 3, 4 g	499:507	2, 3, 4 g per 100 g emulsion; 20 g oil per 100 g emulsion	499:555	Variables were the starch content (2, 3, 4 g per 100 g emulsion; 20 g oil per 100 g emulsion), level of polyglycerol polyricinoleate (PGPR) as a lipophilic emulsifier (0.29, 0.57 g per 100 g emulsion) and ambient-pressure processing temperature for WRS gelatinisation, the non-chemical modification process, (75 ± 3, 88 ± 5 °C).
31259325	2	37	theme	external	412:419	arg1	phases					442:447	external and internal aqueous phases	412:447	external and internal aqueous phases	412:447	Salt levels were 1.5 g salt and 0.47 g salt per 100 g external and internal aqueous phases, respectively.
31259325	0	38	theme	waxy	24:27	arg1	starch					34:39	Non-chemically modified waxy rice starch	0:39	Non-chemically modified waxy rice starch	0:39	Non-chemically modified waxy rice starch stabilised wow emulsions for salt reduction.
31259325	7	39	theme	comparison	1391:1400	arg1	testing					1402:1408	paired comparison testing	1384:1408	paired comparison testing	1384:1408	Salt release was assessed in vitro and through sensory evaluation using paired comparison testing.
31259325	6	40	theme	OSA	1166:1168	arg1	emulsions					1170:1178	OSA emulsions	1166:1178	OSA emulsions	1166:1178	One day after processing, encapsulation efficiency (EE) was as predicted from the composition for OSA emulsions, while at the same PGPR content an external aqueous phase was incorporated into the oil droplets of the WRS emulsion increasing EE.
31259325	9	41	theme	tomato	1580:1585	arg1	soup					1587:1590	a tomato soup	1578:1590	a tomato soup	1578:1590	Consumer tests on a tomato soup, to validate this salt reduction approach for a real food, revealed a possible 25% salt reduction, compared to current UK products.
31259325	0	42	theme	wow	52:54	arg1	emulsions					56:64	wow emulsions	52:64	wow emulsions for salt reduction	52:83	Non-chemically modified waxy rice starch stabilised wow emulsions for salt reduction.
31259325	5	43	theme	low	984:986	arg1	temperature					988:998	lower level PGPR low temperature	967:998	lower level PGPR low temperature	967:998	Emulsions were stable for three months, except OSA and lower level PGPR low temperature processed WRS emulsions lost salt into the external emulsion phase.
31259325	3	44	theme	polyglycerol	568:579	arg1	PGPR					598:601	PGPR	598:601	PGPR	598:601	Variables were the starch content (2, 3, 4 g per 100 g emulsion; 20 g oil per 100 g emulsion), level of polyglycerol polyricinoleate (PGPR) as a lipophilic emulsifier (0.29, 0.57 g per 100 g emulsion) and ambient-pressure processing temperature for WRS gelatinisation, the non-chemical modification process, (75 ± 3, 88 ± 5 °C).
31259325	3	44	theme	polyglycerol	568:579	arg1	polyricinoleate					581:595	polyglycerol polyricinoleate	568:595	polyglycerol polyricinoleate (PGPR) as a lipophilic emulsifier (0.29, 0.57 g per 100 g emulsion)	568:663	Variables were the starch content (2, 3, 4 g per 100 g emulsion; 20 g oil per 100 g emulsion), level of polyglycerol polyricinoleate (PGPR) as a lipophilic emulsifier (0.29, 0.57 g per 100 g emulsion) and ambient-pressure processing temperature for WRS gelatinisation, the non-chemical modification process, (75 ± 3, 88 ± 5 °C).
31259325	6	45	theme	external	1215:1222	arg1	phase					1232:1236	an external aqueous phase	1212:1236	an external aqueous phase	1212:1236	One day after processing, encapsulation efficiency (EE) was as predicted from the composition for OSA emulsions, while at the same PGPR content an external aqueous phase was incorporated into the oil droplets of the WRS emulsion increasing EE.
31259325	5	46	theme	processed	1000:1008	arg1	emulsions					1014:1022	OSA and lower level PGPR low temperature processed WRS emulsions	959:1022	OSA and lower level PGPR low temperature processed WRS emulsions	959:1022	Emulsions were stable for three months, except OSA and lower level PGPR low temperature processed WRS emulsions lost salt into the external emulsion phase.
31259325	9	47	theme	salt	1610:1613	arg1	approach					1625:1632	this salt reduction approach	1605:1632	this salt reduction approach for a real food	1605:1648	Consumer tests on a tomato soup, to validate this salt reduction approach for a real food, revealed a possible 25% salt reduction, compared to current UK products.
31259325	1	48	theme	oral	302:305	arg1	processing					307:316	oral processing	302:316	oral processing due to amylase-induced destabilisation	302:355	Water-in-oil-in-water emulsions containing an internalised salt solution were stabilised with non-chemically modified waxy rice starch (WRS), and octinyl succinic anhydride (OSA) as reference, to release salt during oral processing due to amylase-induced destabilisation.
31259325	3	49	theme	starch	483:488	arg1	content					490:496	the starch content	479:496	the starch content (2, 3, 4 g per 100 g emulsion; 20 g oil per 100 g emulsion)	479:556	Variables were the starch content (2, 3, 4 g per 100 g emulsion; 20 g oil per 100 g emulsion), level of polyglycerol polyricinoleate (PGPR) as a lipophilic emulsifier (0.29, 0.57 g per 100 g emulsion) and ambient-pressure processing temperature for WRS gelatinisation, the non-chemical modification process, (75 ± 3, 88 ± 5 °C).
31259325	3	49	theme	starch	483:488	arg1	Variables					464:472	Variables	464:472	Variables	464:472	Variables were the starch content (2, 3, 4 g per 100 g emulsion; 20 g oil per 100 g emulsion), level of polyglycerol polyricinoleate (PGPR) as a lipophilic emulsifier (0.29, 0.57 g per 100 g emulsion) and ambient-pressure processing temperature for WRS gelatinisation, the non-chemical modification process, (75 ± 3, 88 ± 5 °C).
31259325	8	50	theme	starch	1531:1536	arg1	emulsions					1549:1557	OSA starch stabilised emulsions	1527:1557	OSA starch stabilised emulsions	1527:1557	The results revealed that the efficacy of this salt reduction approach was enhanced for gelatinised WRS compared to OSA starch stabilised emulsions.
31259325	5	51	theme	level	973:977	arg1	temperature					988:998	lower level PGPR low temperature	967:998	lower level PGPR low temperature	967:998	Emulsions were stable for three months, except OSA and lower level PGPR low temperature processed WRS emulsions lost salt into the external emulsion phase.
31259325	1	52	theme	waxy	204:207	arg1	starch					214:219	non-chemically modified waxy rice starch	180:219	non-chemically modified waxy rice starch (WRS)	180:225	Water-in-oil-in-water emulsions containing an internalised salt solution were stabilised with non-chemically modified waxy rice starch (WRS), and octinyl succinic anhydride (OSA) as reference, to release salt during oral processing due to amylase-induced destabilisation.
31259325	1	52	theme	waxy	204:207	arg1	WRS					222:224	WRS	222:224	WRS	222:224	Water-in-oil-in-water emulsions containing an internalised salt solution were stabilised with non-chemically modified waxy rice starch (WRS), and octinyl succinic anhydride (OSA) as reference, to release salt during oral processing due to amylase-induced destabilisation.
31259325	8	53	theme	reduction	1463:1471	arg1	approach					1473:1480	this salt reduction approach	1453:1480	this salt reduction approach	1453:1480	The results revealed that the efficacy of this salt reduction approach was enhanced for gelatinised WRS compared to OSA starch stabilised emulsions.
31259325	6	54	dep	day	1072:1074	arg1	processing					1082:1091	processing	1082:1091	processing	1082:1091	One day after processing, encapsulation efficiency (EE) was as predicted from the composition for OSA emulsions, while at the same PGPR content an external aqueous phase was incorporated into the oil droplets of the WRS emulsion increasing EE.
31259325	7	55	theme	sensory	1359:1365	arg1	evaluation					1367:1376	sensory evaluation	1359:1376	sensory evaluation using paired comparison testing	1359:1408	Salt release was assessed in vitro and through sensory evaluation using paired comparison testing.
31259325	9	56	theme	possible	1662:1669	arg1	reduction					1680:1688	a possible 25% salt reduction	1660:1688	a possible 25% salt reduction	1660:1688	Consumer tests on a tomato soup, to validate this salt reduction approach for a real food, revealed a possible 25% salt reduction, compared to current UK products.
31259325	8	57	theme	approach	1473:1480	arg1	efficacy					1441:1448	the efficacy	1437:1448	the efficacy of this salt reduction approach	1437:1480	The results revealed that the efficacy of this salt reduction approach was enhanced for gelatinised WRS compared to OSA starch stabilised emulsions.
31259325	9	58	theme	%	1673:1673	arg1	reduction					1680:1688	a possible 25% salt reduction	1660:1688	a possible 25% salt reduction	1660:1688	Consumer tests on a tomato soup, to validate this salt reduction approach for a real food, revealed a possible 25% salt reduction, compared to current UK products.
31259325	1	59	contain	containing	118:127	arg1	emulsions					108:116	Water-in-oil-in-water emulsions	86:116	Water-in-oil-in-water emulsions containing an internalised salt solution	86:157	Water-in-oil-in-water emulsions containing an internalised salt solution were stabilised with non-chemically modified waxy rice starch (WRS), and octinyl succinic anhydride (OSA) as reference, to release salt during oral processing due to amylase-induced destabilisation.
31259325	1	59	contain	containing	118:127	arg2	solution					150:157	an internalised salt solution	129:157	an internalised salt solution	129:157	Water-in-oil-in-water emulsions containing an internalised salt solution were stabilised with non-chemically modified waxy rice starch (WRS), and octinyl succinic anhydride (OSA) as reference, to release salt during oral processing due to amylase-induced destabilisation.
31259325	4	60	dep	conditions	838:847	arg1	PGPR					875:878	0.57 g PGPR	868:878	0.57 g PGPR per 100 g emulsion	868:897	OSA starch was used under previously applied conditions (2, 3, 4 g starch, 0.57 g PGPR per 100 g emulsion, 25 ± 5 °C).
31259325	4	60	dep	conditions	838:847	arg1	g					858:858	2, 3, 4 g	850:858	2, 3, 4 g starch	850:865	OSA starch was used under previously applied conditions (2, 3, 4 g starch, 0.57 g PGPR per 100 g emulsion, 25 ± 5 °C).
31259325	4	60	dep	conditions	838:847	arg1	°C					907:908	25 ± 5 °C	900:908	25 ± 5 °C	900:908	OSA starch was used under previously applied conditions (2, 3, 4 g starch, 0.57 g PGPR per 100 g emulsion, 25 ± 5 °C).
31259325	3	61	theme	emulsion	519:526	arg1	oil					534:536	100 g emulsion; 20 g oil	513:536	100 g emulsion; 20 g oil	513:536	Variables were the starch content (2, 3, 4 g per 100 g emulsion; 20 g oil per 100 g emulsion), level of polyglycerol polyricinoleate (PGPR) as a lipophilic emulsifier (0.29, 0.57 g per 100 g emulsion) and ambient-pressure processing temperature for WRS gelatinisation, the non-chemical modification process, (75 ± 3, 88 ± 5 °C).
31259325	1	62	theme	internalised	132:143	arg1	solution					150:157	an internalised salt solution	129:157	an internalised salt solution	129:157	Water-in-oil-in-water emulsions containing an internalised salt solution were stabilised with non-chemically modified waxy rice starch (WRS), and octinyl succinic anhydride (OSA) as reference, to release salt during oral processing due to amylase-induced destabilisation.
31259325	3	63	theme	g	653:653	arg1	emulsion					655:662	100 g emulsion	649:662	100 g emulsion	649:662	Variables were the starch content (2, 3, 4 g per 100 g emulsion; 20 g oil per 100 g emulsion), level of polyglycerol polyricinoleate (PGPR) as a lipophilic emulsifier (0.29, 0.57 g per 100 g emulsion) and ambient-pressure processing temperature for WRS gelatinisation, the non-chemical modification process, (75 ± 3, 88 ± 5 °C).
31259325	4	64	used	used	808:811	arg2	starch					797:802	OSA starch	793:802	OSA starch	793:802	OSA starch was used under previously applied conditions (2, 3, 4 g starch, 0.57 g PGPR per 100 g emulsion, 25 ± 5 °C).
31259325	5	65	theme	emulsion	1052:1059	arg1	phase					1061:1065	the external emulsion phase	1039:1065	the external emulsion phase	1039:1065	Emulsions were stable for three months, except OSA and lower level PGPR low temperature processed WRS emulsions lost salt into the external emulsion phase.
31259325	0	66	theme	salt	70:73	arg1	reduction					75:83	salt reduction	70:83	salt reduction	70:83	Non-chemically modified waxy rice starch stabilised wow emulsions for salt reduction.
31259325	6	67	theme	EE	1308:1309	arg1	droplets					1268:1275	the oil droplets	1260:1275	the oil droplets of the WRS emulsion increasing EE	1260:1309	One day after processing, encapsulation efficiency (EE) was as predicted from the composition for OSA emulsions, while at the same PGPR content an external aqueous phase was incorporated into the oil droplets of the WRS emulsion increasing EE.
31259325	3	68	theme	non-chemical	737:748	arg1	process					763:769	the non-chemical modification process	733:769	the non-chemical modification process	733:769	Variables were the starch content (2, 3, 4 g per 100 g emulsion; 20 g oil per 100 g emulsion), level of polyglycerol polyricinoleate (PGPR) as a lipophilic emulsifier (0.29, 0.57 g per 100 g emulsion) and ambient-pressure processing temperature for WRS gelatinisation, the non-chemical modification process, (75 ± 3, 88 ± 5 °C).
31259325	3	68	theme	non-chemical	737:748	arg1	gelatinisation					717:730	gelatinisation	717:730	gelatinisation	717:730	Variables were the starch content (2, 3, 4 g per 100 g emulsion; 20 g oil per 100 g emulsion), level of polyglycerol polyricinoleate (PGPR) as a lipophilic emulsifier (0.29, 0.57 g per 100 g emulsion) and ambient-pressure processing temperature for WRS gelatinisation, the non-chemical modification process, (75 ± 3, 88 ± 5 °C).
31259325	7	69	theme	Salt	1312:1315	arg1	release					1317:1323	Salt release	1312:1323	Salt release	1312:1323	Salt release was assessed in vitro and through sensory evaluation using paired comparison testing.
31259325	9	70	theme	current	1703:1709	arg1	products					1714:1721	current UK products	1703:1721	current UK products	1703:1721	Consumer tests on a tomato soup, to validate this salt reduction approach for a real food, revealed a possible 25% salt reduction, compared to current UK products.
31259325	1	71	theme	octinyl	232:238	arg1	anhydride					249:257	octinyl succinic anhydride	232:257	octinyl succinic anhydride (OSA)	232:263	Water-in-oil-in-water emulsions containing an internalised salt solution were stabilised with non-chemically modified waxy rice starch (WRS), and octinyl succinic anhydride (OSA) as reference, to release salt during oral processing due to amylase-induced destabilisation.
31259325	1	71	theme	octinyl	232:238	arg1	OSA					260:262	OSA	260:262	OSA	260:262	Water-in-oil-in-water emulsions containing an internalised salt solution were stabilised with non-chemically modified waxy rice starch (WRS), and octinyl succinic anhydride (OSA) as reference, to release salt during oral processing due to amylase-induced destabilisation.
31259325	3	72	theme	ambient-pressure	669:684	arg1	temperature					697:707	ambient-pressure processing temperature	669:707	ambient-pressure processing temperature for WRS gelatinisation, the non-chemical modification process, (75 ± 3, 88 ± 5 °C)	669:790	Variables were the starch content (2, 3, 4 g per 100 g emulsion; 20 g oil per 100 g emulsion), level of polyglycerol polyricinoleate (PGPR) as a lipophilic emulsifier (0.29, 0.57 g per 100 g emulsion) and ambient-pressure processing temperature for WRS gelatinisation, the non-chemical modification process, (75 ± 3, 88 ± 5 °C).
31259325	1	73	theme	amylase-induced	325:339	arg1	destabilisation					341:355	amylase-induced destabilisation	325:355	amylase-induced destabilisation	325:355	Water-in-oil-in-water emulsions containing an internalised salt solution were stabilised with non-chemically modified waxy rice starch (WRS), and octinyl succinic anhydride (OSA) as reference, to release salt during oral processing due to amylase-induced destabilisation.
31259325	6	74	theme	emulsion	1288:1295	arg1	EE					1308:1309	the WRS emulsion increasing EE	1280:1309	the WRS emulsion increasing EE	1280:1309	One day after processing, encapsulation efficiency (EE) was as predicted from the composition for OSA emulsions, while at the same PGPR content an external aqueous phase was incorporated into the oil droplets of the WRS emulsion increasing EE.
31259325	4	75	theme	applied	830:836	arg1	conditions					838:847	previously applied conditions	819:847	previously applied conditions (2, 3, 4 g starch, 0.57 g PGPR per 100 g emulsion, 25 ± 5 °C)	819:909	OSA starch was used under previously applied conditions (2, 3, 4 g starch, 0.57 g PGPR per 100 g emulsion, 25 ± 5 °C).
31259325	4	76	theme	OSA	793:795	arg1	starch					797:802	OSA starch	793:802	OSA starch	793:802	OSA starch was used under previously applied conditions (2, 3, 4 g starch, 0.57 g PGPR per 100 g emulsion, 25 ± 5 °C).
31259325	1	77	theme	succinic	240:247	arg1	anhydride					249:257	octinyl succinic anhydride	232:257	octinyl succinic anhydride (OSA)	232:263	Water-in-oil-in-water emulsions containing an internalised salt solution were stabilised with non-chemically modified waxy rice starch (WRS), and octinyl succinic anhydride (OSA) as reference, to release salt during oral processing due to amylase-induced destabilisation.
31259325	1	77	theme	succinic	240:247	arg1	OSA					260:262	OSA	260:262	OSA	260:262	Water-in-oil-in-water emulsions containing an internalised salt solution were stabilised with non-chemically modified waxy rice starch (WRS), and octinyl succinic anhydride (OSA) as reference, to release salt during oral processing due to amylase-induced destabilisation.
31259325	0	78	theme	modified	15:22	arg1	starch					34:39	Non-chemically modified waxy rice starch	0:39	Non-chemically modified waxy rice starch	0:39	Non-chemically modified waxy rice starch stabilised wow emulsions for salt reduction.
31259325	4	79	dep	g	888:888	arg1	emulsion					890:897	emulsion	890:897	100 g emulsion	884:897	OSA starch was used under previously applied conditions (2, 3, 4 g starch, 0.57 g PGPR per 100 g emulsion, 25 ± 5 °C).
31259325	1	80	theme	Water-in-oil-in-water	86:106	arg1	emulsions					108:116	Water-in-oil-in-water emulsions	86:116	Water-in-oil-in-water emulsions containing an internalised salt solution	86:157	Water-in-oil-in-water emulsions containing an internalised salt solution were stabilised with non-chemically modified waxy rice starch (WRS), and octinyl succinic anhydride (OSA) as reference, to release salt during oral processing due to amylase-induced destabilisation.
31259325	0	81	theme	rice	29:32	arg1	starch					34:39	Non-chemically modified waxy rice starch	0:39	Non-chemically modified waxy rice starch	0:39	Non-chemically modified waxy rice starch stabilised wow emulsions for salt reduction.
31259325	4	82	theme	±	903:903	arg1	g					858:858	2, 3, 4 g	850:858	2, 3, 4 g starch	850:865	OSA starch was used under previously applied conditions (2, 3, 4 g starch, 0.57 g PGPR per 100 g emulsion, 25 ± 5 °C).
31259325	4	82	theme	±	903:903	arg1	°C					907:908	25 ± 5 °C	900:908	25 ± 5 °C	900:908	OSA starch was used under previously applied conditions (2, 3, 4 g starch, 0.57 g PGPR per 100 g emulsion, 25 ± 5 °C).
31259325	5	83	theme	OSA	959:961	arg1	emulsions					1014:1022	OSA and lower level PGPR low temperature processed WRS emulsions	959:1022	OSA and lower level PGPR low temperature processed WRS emulsions	959:1022	Emulsions were stable for three months, except OSA and lower level PGPR low temperature processed WRS emulsions lost salt into the external emulsion phase.
31259325	2	84	theme	g	395:395	arg1	salt					397:400	0.47 g salt	390:400	0.47 g salt per 100 g	390:410	Salt levels were 1.5 g salt and 0.47 g salt per 100 g external and internal aqueous phases, respectively.
31259325	3	85	dep	emulsifier	620:629	arg1	g					643:643	0.29, 0.57 g	632:643	0.29, 0.57 g per 100 g emulsion	632:662	Variables were the starch content (2, 3, 4 g per 100 g emulsion; 20 g oil per 100 g emulsion), level of polyglycerol polyricinoleate (PGPR) as a lipophilic emulsifier (0.29, 0.57 g per 100 g emulsion) and ambient-pressure processing temperature for WRS gelatinisation, the non-chemical modification process, (75 ± 3, 88 ± 5 °C).
31259325	2	86	theme	aqueous	434:440	arg1	phases					442:447	external and internal aqueous phases	412:447	external and internal aqueous phases	412:447	Salt levels were 1.5 g salt and 0.47 g salt per 100 g external and internal aqueous phases, respectively.
31259325	3	87	theme	polyricinoleate	581:595	arg1	content					490:496	the starch content	479:496	the starch content (2, 3, 4 g per 100 g emulsion; 20 g oil per 100 g emulsion)	479:556	Variables were the starch content (2, 3, 4 g per 100 g emulsion; 20 g oil per 100 g emulsion), level of polyglycerol polyricinoleate (PGPR) as a lipophilic emulsifier (0.29, 0.57 g per 100 g emulsion) and ambient-pressure processing temperature for WRS gelatinisation, the non-chemical modification process, (75 ± 3, 88 ± 5 °C).
31259325	3	87	theme	polyricinoleate	581:595	arg1	level					559:563	level	559:563	level of polyglycerol polyricinoleate (PGPR) as a lipophilic emulsifier (0.29, 0.57 g per 100 g emulsion)	559:663	Variables were the starch content (2, 3, 4 g per 100 g emulsion; 20 g oil per 100 g emulsion), level of polyglycerol polyricinoleate (PGPR) as a lipophilic emulsifier (0.29, 0.57 g per 100 g emulsion) and ambient-pressure processing temperature for WRS gelatinisation, the non-chemical modification process, (75 ± 3, 88 ± 5 °C).
31259325	3	87	theme	polyricinoleate	581:595	arg1	Variables					464:472	Variables	464:472	Variables	464:472	Variables were the starch content (2, 3, 4 g per 100 g emulsion; 20 g oil per 100 g emulsion), level of polyglycerol polyricinoleate (PGPR) as a lipophilic emulsifier (0.29, 0.57 g per 100 g emulsion) and ambient-pressure processing temperature for WRS gelatinisation, the non-chemical modification process, (75 ± 3, 88 ± 5 °C).
31259325	3	87	theme	polyricinoleate	581:595	arg1	temperature					697:707	ambient-pressure processing temperature	669:707	ambient-pressure processing temperature for WRS gelatinisation, the non-chemical modification process, (75 ± 3, 88 ± 5 °C)	669:790	Variables were the starch content (2, 3, 4 g per 100 g emulsion; 20 g oil per 100 g emulsion), level of polyglycerol polyricinoleate (PGPR) as a lipophilic emulsifier (0.29, 0.57 g per 100 g emulsion) and ambient-pressure processing temperature for WRS gelatinisation, the non-chemical modification process, (75 ± 3, 88 ± 5 °C).
31259325	3	88	theme	g	532:532	arg1	oil					534:536	100 g emulsion; 20 g oil	513:536	100 g emulsion; 20 g oil	513:536	Variables were the starch content (2, 3, 4 g per 100 g emulsion; 20 g oil per 100 g emulsion), level of polyglycerol polyricinoleate (PGPR) as a lipophilic emulsifier (0.29, 0.57 g per 100 g emulsion) and ambient-pressure processing temperature for WRS gelatinisation, the non-chemical modification process, (75 ± 3, 88 ± 5 °C).
31259325	8	89	dep	starch	1531:1536	arg1	OSA					1527:1529	OSA	1527:1529	OSA	1527:1529	The results revealed that the efficacy of this salt reduction approach was enhanced for gelatinised WRS compared to OSA starch stabilised emulsions.
31259325	8	89	dep	starch	1531:1536	arg1	stabilised					1538:1547	stabilised	1538:1547	stabilised	1538:1547	The results revealed that the efficacy of this salt reduction approach was enhanced for gelatinised WRS compared to OSA starch stabilised emulsions.
31259325	2	90	dep	g	379:379	arg1	phases					442:447	external and internal aqueous phases	412:447	external and internal aqueous phases	412:447	Salt levels were 1.5 g salt and 0.47 g salt per 100 g external and internal aqueous phases, respectively.
31259325	2	90	dep	g	379:379	arg1	salt					381:384	salt	381:384	1.5 g salt	375:384	Salt levels were 1.5 g salt and 0.47 g salt per 100 g external and internal aqueous phases, respectively.
31259325	6	91	theme	aqueous	1224:1230	arg1	phase					1232:1236	an external aqueous phase	1212:1236	an external aqueous phase	1212:1236	One day after processing, encapsulation efficiency (EE) was as predicted from the composition for OSA emulsions, while at the same PGPR content an external aqueous phase was incorporated into the oil droplets of the WRS emulsion increasing EE.
31259325	5	92	theme	temperature	988:998	arg1	emulsions					1014:1022	OSA and lower level PGPR low temperature processed WRS emulsions	959:1022	OSA and lower level PGPR low temperature processed WRS emulsions	959:1022	Emulsions were stable for three months, except OSA and lower level PGPR low temperature processed WRS emulsions lost salt into the external emulsion phase.
31259325	4	93	theme	0.57	868:871	arg1	PGPR					875:878	0.57 g PGPR	868:878	0.57 g PGPR per 100 g emulsion	868:897	OSA starch was used under previously applied conditions (2, 3, 4 g starch, 0.57 g PGPR per 100 g emulsion, 25 ± 5 °C).
31259325	4	93	theme	0.57	868:871	arg1	g					858:858	2, 3, 4 g	850:858	2, 3, 4 g starch	850:865	OSA starch was used under previously applied conditions (2, 3, 4 g starch, 0.57 g PGPR per 100 g emulsion, 25 ± 5 °C).
31259325	5	94	theme	WRS	1010:1012	arg1	emulsions					1014:1022	OSA and lower level PGPR low temperature processed WRS emulsions	959:1022	OSA and lower level PGPR low temperature processed WRS emulsions	959:1022	Emulsions were stable for three months, except OSA and lower level PGPR low temperature processed WRS emulsions lost salt into the external emulsion phase.
31279124	3	0	theme	Ca	780:781	arg1	rate					826:829	the hydroxyl radical scavenging rate	794:829	the hydroxyl radical scavenging rate	794:829	A chemical component analysis showed that in sprouted black barley, the HRW treatment could change the distribution of phytochemicals (e.g., the ionic strength of guanosine), increase the concentrations of free vanillic acid, coumaric acid, sinapic acid, conjugated sinapic acid, Ca and Fe and the hydroxyl radical scavenging rate, and decrease the protein, fat, starch and dietary fibre contents compared with the results obtained after treatment with ultra-pure water (P < 0.05).
31279124	3	0	theme	Ca	780:781	arg1	concentrations					688:701	the concentrations	684:701	the concentrations of free vanillic acid, coumaric acid, sinapic acid, conjugated sinapic acid, Ca and Fe	684:788	A chemical component analysis showed that in sprouted black barley, the HRW treatment could change the distribution of phytochemicals (e.g., the ionic strength of guanosine), increase the concentrations of free vanillic acid, coumaric acid, sinapic acid, conjugated sinapic acid, Ca and Fe and the hydroxyl radical scavenging rate, and decrease the protein, fat, starch and dietary fibre contents compared with the results obtained after treatment with ultra-pure water (P < 0.05).
31279124	3	1	theme	acid	735:738	arg1	rate					826:829	the hydroxyl radical scavenging rate	794:829	the hydroxyl radical scavenging rate	794:829	A chemical component analysis showed that in sprouted black barley, the HRW treatment could change the distribution of phytochemicals (e.g., the ionic strength of guanosine), increase the concentrations of free vanillic acid, coumaric acid, sinapic acid, conjugated sinapic acid, Ca and Fe and the hydroxyl radical scavenging rate, and decrease the protein, fat, starch and dietary fibre contents compared with the results obtained after treatment with ultra-pure water (P < 0.05).
31279124	3	1	theme	acid	735:738	arg1	concentrations					688:701	the concentrations	684:701	the concentrations of free vanillic acid, coumaric acid, sinapic acid, conjugated sinapic acid, Ca and Fe	684:788	A chemical component analysis showed that in sprouted black barley, the HRW treatment could change the distribution of phytochemicals (e.g., the ionic strength of guanosine), increase the concentrations of free vanillic acid, coumaric acid, sinapic acid, conjugated sinapic acid, Ca and Fe and the hydroxyl radical scavenging rate, and decrease the protein, fat, starch and dietary fibre contents compared with the results obtained after treatment with ultra-pure water (P < 0.05).
31279124	2	2	dep	rate	451:454	arg1	the					435:437	the	435:437	the	435:437	In the present study, hydrogen-rich water (HRW) (2 ppm) was used for the processing of sprouted black barley (Hordeum distichum L.), and the results showed that the HRW treatment could significantly increase the germination rate and growth rate of black barley (P < 0.05).
31279124	3	3	theme	dietary	874:880	arg1	contents					888:895	dietary fibre contents	874:895	dietary fibre contents	874:895	A chemical component analysis showed that in sprouted black barley, the HRW treatment could change the distribution of phytochemicals (e.g., the ionic strength of guanosine), increase the concentrations of free vanillic acid, coumaric acid, sinapic acid, conjugated sinapic acid, Ca and Fe and the hydroxyl radical scavenging rate, and decrease the protein, fat, starch and dietary fibre contents compared with the results obtained after treatment with ultra-pure water (P < 0.05).
31279124	1	4	theme	multifunctional	140:154	arg1	molecule					166:173	a multifunctional signaling molecule	138:173	a multifunctional signaling molecule	138:173	Hydrogen gas (H2), a multifunctional signaling molecule, has received increasing attention in recent years.
31279124	1	4	theme	multifunctional	140:154	arg1	gas					128:130	Hydrogen gas	119:130	Hydrogen gas (H2)	119:135	Hydrogen gas (H2), a multifunctional signaling molecule, has received increasing attention in recent years.
31279124	3	5	theme	sinapic	766:772	arg1	acid					774:777	conjugated sinapic acid	755:777	conjugated sinapic acid	755:777	A chemical component analysis showed that in sprouted black barley, the HRW treatment could change the distribution of phytochemicals (e.g., the ionic strength of guanosine), increase the concentrations of free vanillic acid, coumaric acid, sinapic acid, conjugated sinapic acid, Ca and Fe and the hydroxyl radical scavenging rate, and decrease the protein, fat, starch and dietary fibre contents compared with the results obtained after treatment with ultra-pure water (P < 0.05).
31279124	0	6	theme	black	105:109	arg1	barley					111:116	sprouted black barley	96:116	sprouted black barley	96:116	Effects of hydrogen-rich water on the nutrient composition and antioxidative characteristics of sprouted black barley.
31279124	0	7	from	Effects	0:6	arg1	characteristics					77:91	antioxidative characteristics	63:91	antioxidative characteristics	63:91	Effects of hydrogen-rich water on the nutrient composition and antioxidative characteristics of sprouted black barley.
31279124	0	7	from	Effects	0:6	arg1	composition					47:57	nutrient composition	38:57	nutrient composition	38:57	Effects of hydrogen-rich water on the nutrient composition and antioxidative characteristics of sprouted black barley.
31279124	3	8	theme	fibre	882:886	arg1	contents					888:895	dietary fibre contents	874:895	dietary fibre contents	874:895	A chemical component analysis showed that in sprouted black barley, the HRW treatment could change the distribution of phytochemicals (e.g., the ionic strength of guanosine), increase the concentrations of free vanillic acid, coumaric acid, sinapic acid, conjugated sinapic acid, Ca and Fe and the hydroxyl radical scavenging rate, and decrease the protein, fat, starch and dietary fibre contents compared with the results obtained after treatment with ultra-pure water (P < 0.05).
31279124	3	9	theme	acid	774:777	arg1	rate					826:829	the hydroxyl radical scavenging rate	794:829	the hydroxyl radical scavenging rate	794:829	A chemical component analysis showed that in sprouted black barley, the HRW treatment could change the distribution of phytochemicals (e.g., the ionic strength of guanosine), increase the concentrations of free vanillic acid, coumaric acid, sinapic acid, conjugated sinapic acid, Ca and Fe and the hydroxyl radical scavenging rate, and decrease the protein, fat, starch and dietary fibre contents compared with the results obtained after treatment with ultra-pure water (P < 0.05).
31279124	3	9	theme	acid	774:777	arg1	concentrations					688:701	the concentrations	684:701	the concentrations of free vanillic acid, coumaric acid, sinapic acid, conjugated sinapic acid, Ca and Fe	684:788	A chemical component analysis showed that in sprouted black barley, the HRW treatment could change the distribution of phytochemicals (e.g., the ionic strength of guanosine), increase the concentrations of free vanillic acid, coumaric acid, sinapic acid, conjugated sinapic acid, Ca and Fe and the hydroxyl radical scavenging rate, and decrease the protein, fat, starch and dietary fibre contents compared with the results obtained after treatment with ultra-pure water (P < 0.05).
31279124	3	10	dep	change	592:597	arg1	decrease					836:843	decrease	836:843	decrease the protein, fat, starch and dietary fibre contents compared with the results obtained after treatment with ultra-pure water (P < 0.05)	836:979	A chemical component analysis showed that in sprouted black barley, the HRW treatment could change the distribution of phytochemicals (e.g., the ionic strength of guanosine), increase the concentrations of free vanillic acid, coumaric acid, sinapic acid, conjugated sinapic acid, Ca and Fe and the hydroxyl radical scavenging rate, and decrease the protein, fat, starch and dietary fibre contents compared with the results obtained after treatment with ultra-pure water (P < 0.05).
31279124	3	10	dep	change	592:597	arg1	increase					675:682	increase	675:682	increase the concentrations of free vanillic acid, coumaric acid, sinapic acid, conjugated sinapic acid, Ca and Fe and the hydroxyl radical scavenging rate	675:829	A chemical component analysis showed that in sprouted black barley, the HRW treatment could change the distribution of phytochemicals (e.g., the ionic strength of guanosine), increase the concentrations of free vanillic acid, coumaric acid, sinapic acid, conjugated sinapic acid, Ca and Fe and the hydroxyl radical scavenging rate, and decrease the protein, fat, starch and dietary fibre contents compared with the results obtained after treatment with ultra-pure water (P < 0.05).
31279124	2	11	theme	growth	460:465	arg1	rate					467:470	growth rate	460:470	growth rate	460:470	In the present study, hydrogen-rich water (HRW) (2 ppm) was used for the processing of sprouted black barley (Hordeum distichum L.), and the results showed that the HRW treatment could significantly increase the germination rate and growth rate of black barley (P < 0.05).
31279124	4	12	theme	phytochemicals	1121:1134	arg1	efficiency					1078:1087	their germination efficiency	1060:1087	their germination efficiency	1060:1087	HRW can be used for the processing of sprouted grains to effectively increase their germination efficiency and concentrations of bioactive phytochemicals.
31279124	4	12	theme	phytochemicals	1121:1134	arg1	concentrations					1093:1106	concentrations	1093:1106	concentrations	1093:1106	HRW can be used for the processing of sprouted grains to effectively increase their germination efficiency and concentrations of bioactive phytochemicals.
31279124	3	13	theme	coumaric	726:733	arg1	acid					735:738	coumaric acid	726:738	coumaric acid	726:738	A chemical component analysis showed that in sprouted black barley, the HRW treatment could change the distribution of phytochemicals (e.g., the ionic strength of guanosine), increase the concentrations of free vanillic acid, coumaric acid, sinapic acid, conjugated sinapic acid, Ca and Fe and the hydroxyl radical scavenging rate, and decrease the protein, fat, starch and dietary fibre contents compared with the results obtained after treatment with ultra-pure water (P < 0.05).
31279124	3	14	theme	ionic	645:649	arg1	strength					651:658	the ionic strength	641:658	the ionic strength of guanosine	641:671	A chemical component analysis showed that in sprouted black barley, the HRW treatment could change the distribution of phytochemicals (e.g., the ionic strength of guanosine), increase the concentrations of free vanillic acid, coumaric acid, sinapic acid, conjugated sinapic acid, Ca and Fe and the hydroxyl radical scavenging rate, and decrease the protein, fat, starch and dietary fibre contents compared with the results obtained after treatment with ultra-pure water (P < 0.05).
31279124	4	15	theme	bioactive	1111:1119	arg1	phytochemicals					1121:1134	bioactive phytochemicals	1111:1134	bioactive phytochemicals	1111:1134	HRW can be used for the processing of sprouted grains to effectively increase their germination efficiency and concentrations of bioactive phytochemicals.
31279124	1	16	theme	signaling	156:164	arg1	molecule					166:173	a multifunctional signaling molecule	138:173	a multifunctional signaling molecule	138:173	Hydrogen gas (H2), a multifunctional signaling molecule, has received increasing attention in recent years.
31279124	1	16	theme	signaling	156:164	arg1	gas					128:130	Hydrogen gas	119:130	Hydrogen gas (H2)	119:135	Hydrogen gas (H2), a multifunctional signaling molecule, has received increasing attention in recent years.
31279124	0	17	theme	barley	111:116	arg1	characteristics					77:91	antioxidative characteristics	63:91	antioxidative characteristics	63:91	Effects of hydrogen-rich water on the nutrient composition and antioxidative characteristics of sprouted black barley.
31279124	0	17	theme	barley	111:116	arg1	composition					47:57	nutrient composition	38:57	nutrient composition	38:57	Effects of hydrogen-rich water on the nutrient composition and antioxidative characteristics of sprouted black barley.
31279124	4	18	theme	germination	1066:1076	arg1	efficiency					1078:1087	their germination efficiency	1060:1087	their germination efficiency	1060:1087	HRW can be used for the processing of sprouted grains to effectively increase their germination efficiency and concentrations of bioactive phytochemicals.
31279124	2	19	theme	hydrogen-rich	249:261	arg1	HRW					270:272	HRW	270:272	HRW	270:272	In the present study, hydrogen-rich water (HRW) (2 ppm) was used for the processing of sprouted black barley (Hordeum distichum L.), and the results showed that the HRW treatment could significantly increase the germination rate and growth rate of black barley (P < 0.05).
31279124	2	19	theme	hydrogen-rich	249:261	arg1	2 ppm					276:280	2 ppm	276:280	2 ppm	276:280	In the present study, hydrogen-rich water (HRW) (2 ppm) was used for the processing of sprouted black barley (Hordeum distichum L.), and the results showed that the HRW treatment could significantly increase the germination rate and growth rate of black barley (P < 0.05).
31279124	2	19	theme	hydrogen-rich	249:261	arg1	water					263:267	hydrogen-rich water	249:267	hydrogen-rich water (HRW) (2 ppm)	249:281	In the present study, hydrogen-rich water (HRW) (2 ppm) was used for the processing of sprouted black barley (Hordeum distichum L.), and the results showed that the HRW treatment could significantly increase the germination rate and growth rate of black barley (P < 0.05).
31279124	2	20	used	used	287:290	arg2	2 ppm					276:280	2 ppm	276:280	2 ppm	276:280	In the present study, hydrogen-rich water (HRW) (2 ppm) was used for the processing of sprouted black barley (Hordeum distichum L.), and the results showed that the HRW treatment could significantly increase the germination rate and growth rate of black barley (P < 0.05).
31279124	2	20	used	used	287:290	arg2	HRW					270:272	HRW	270:272	HRW	270:272	In the present study, hydrogen-rich water (HRW) (2 ppm) was used for the processing of sprouted black barley (Hordeum distichum L.), and the results showed that the HRW treatment could significantly increase the germination rate and growth rate of black barley (P < 0.05).
31279124	2	20	used	used	287:290	arg2	water					263:267	hydrogen-rich water	249:267	hydrogen-rich water (HRW) (2 ppm)	249:281	In the present study, hydrogen-rich water (HRW) (2 ppm) was used for the processing of sprouted black barley (Hordeum distichum L.), and the results showed that the HRW treatment could significantly increase the germination rate and growth rate of black barley (P < 0.05).
31279124	3	21	theme	Fe	787:788	arg1	rate					826:829	the hydroxyl radical scavenging rate	794:829	the hydroxyl radical scavenging rate	794:829	A chemical component analysis showed that in sprouted black barley, the HRW treatment could change the distribution of phytochemicals (e.g., the ionic strength of guanosine), increase the concentrations of free vanillic acid, coumaric acid, sinapic acid, conjugated sinapic acid, Ca and Fe and the hydroxyl radical scavenging rate, and decrease the protein, fat, starch and dietary fibre contents compared with the results obtained after treatment with ultra-pure water (P < 0.05).
31279124	3	21	theme	Fe	787:788	arg1	concentrations					688:701	the concentrations	684:701	the concentrations of free vanillic acid, coumaric acid, sinapic acid, conjugated sinapic acid, Ca and Fe	684:788	A chemical component analysis showed that in sprouted black barley, the HRW treatment could change the distribution of phytochemicals (e.g., the ionic strength of guanosine), increase the concentrations of free vanillic acid, coumaric acid, sinapic acid, conjugated sinapic acid, Ca and Fe and the hydroxyl radical scavenging rate, and decrease the protein, fat, starch and dietary fibre contents compared with the results obtained after treatment with ultra-pure water (P < 0.05).
31279124	2	22	theme	black	475:479	arg1	barley					481:486	black barley	475:486	black barley (P < 0.05)	475:497	In the present study, hydrogen-rich water (HRW) (2 ppm) was used for the processing of sprouted black barley (Hordeum distichum L.), and the results showed that the HRW treatment could significantly increase the germination rate and growth rate of black barley (P < 0.05).
31279124	2	22	theme	black	475:479	arg1	P < 0.05					489:496	P < 0.05	489:496	P < 0.05	489:496	In the present study, hydrogen-rich water (HRW) (2 ppm) was used for the processing of sprouted black barley (Hordeum distichum L.), and the results showed that the HRW treatment could significantly increase the germination rate and growth rate of black barley (P < 0.05).
31279124	3	23	theme	guanosine	663:671	arg1	strength					651:658	the ionic strength	641:658	the ionic strength of guanosine	641:671	A chemical component analysis showed that in sprouted black barley, the HRW treatment could change the distribution of phytochemicals (e.g., the ionic strength of guanosine), increase the concentrations of free vanillic acid, coumaric acid, sinapic acid, conjugated sinapic acid, Ca and Fe and the hydroxyl radical scavenging rate, and decrease the protein, fat, starch and dietary fibre contents compared with the results obtained after treatment with ultra-pure water (P < 0.05).
31279124	3	24	theme	ultra-pure	953:962	arg1	P < 0.05					971:978	P < 0.05	971:978	P < 0.05	971:978	A chemical component analysis showed that in sprouted black barley, the HRW treatment could change the distribution of phytochemicals (e.g., the ionic strength of guanosine), increase the concentrations of free vanillic acid, coumaric acid, sinapic acid, conjugated sinapic acid, Ca and Fe and the hydroxyl radical scavenging rate, and decrease the protein, fat, starch and dietary fibre contents compared with the results obtained after treatment with ultra-pure water (P < 0.05).
31279124	3	24	theme	ultra-pure	953:962	arg1	water					964:968	ultra-pure water	953:968	ultra-pure water (P < 0.05)	953:979	A chemical component analysis showed that in sprouted black barley, the HRW treatment could change the distribution of phytochemicals (e.g., the ionic strength of guanosine), increase the concentrations of free vanillic acid, coumaric acid, sinapic acid, conjugated sinapic acid, Ca and Fe and the hydroxyl radical scavenging rate, and decrease the protein, fat, starch and dietary fibre contents compared with the results obtained after treatment with ultra-pure water (P < 0.05).
31279124	3	25	theme	scavenging	815:824	arg1	rate					826:829	the hydroxyl radical scavenging rate	794:829	the hydroxyl radical scavenging rate	794:829	A chemical component analysis showed that in sprouted black barley, the HRW treatment could change the distribution of phytochemicals (e.g., the ionic strength of guanosine), increase the concentrations of free vanillic acid, coumaric acid, sinapic acid, conjugated sinapic acid, Ca and Fe and the hydroxyl radical scavenging rate, and decrease the protein, fat, starch and dietary fibre contents compared with the results obtained after treatment with ultra-pure water (P < 0.05).
31279124	2	26	theme	barley	329:334	arg1	processing					300:309	the processing	296:309	the processing of sprouted black barley (Hordeum distichum L.)	296:357	In the present study, hydrogen-rich water (HRW) (2 ppm) was used for the processing of sprouted black barley (Hordeum distichum L.), and the results showed that the HRW treatment could significantly increase the germination rate and growth rate of black barley (P < 0.05).
31279124	0	27	theme	water	25:29	arg1	Effects					0:6	Effects	0:6	Effects of hydrogen-rich water on the nutrient composition and antioxidative characteristics of sprouted black barley	0:116	Effects of hydrogen-rich water on the nutrient composition and antioxidative characteristics of sprouted black barley.
31279124	4	28	used	used	993:996	arg2	HRW					982:984	HRW	982:984	HRW	982:984	HRW can be used for the processing of sprouted grains to effectively increase their germination efficiency and concentrations of bioactive phytochemicals.
31279124	0	29	theme	hydrogen-rich	11:23	arg1	water					25:29	hydrogen-rich water	11:29	hydrogen-rich water	11:29	Effects of hydrogen-rich water on the nutrient composition and antioxidative characteristics of sprouted black barley.
31279124	2	30	theme	barley	481:486	arg1	rate					451:454	germination rate	439:454	germination rate	439:454	In the present study, hydrogen-rich water (HRW) (2 ppm) was used for the processing of sprouted black barley (Hordeum distichum L.), and the results showed that the HRW treatment could significantly increase the germination rate and growth rate of black barley (P < 0.05).
31279124	2	30	theme	barley	481:486	arg1	rate					467:470	growth rate	460:470	growth rate	460:470	In the present study, hydrogen-rich water (HRW) (2 ppm) was used for the processing of sprouted black barley (Hordeum distichum L.), and the results showed that the HRW treatment could significantly increase the germination rate and growth rate of black barley (P < 0.05).
31279124	3	31	with	treatment	938:946	arg1	P < 0.05					971:978	P < 0.05	971:978	P < 0.05	971:978	A chemical component analysis showed that in sprouted black barley, the HRW treatment could change the distribution of phytochemicals (e.g., the ionic strength of guanosine), increase the concentrations of free vanillic acid, coumaric acid, sinapic acid, conjugated sinapic acid, Ca and Fe and the hydroxyl radical scavenging rate, and decrease the protein, fat, starch and dietary fibre contents compared with the results obtained after treatment with ultra-pure water (P < 0.05).
31279124	3	31	with	treatment	938:946	arg1	water					964:968	ultra-pure water	953:968	ultra-pure water (P < 0.05)	953:979	A chemical component analysis showed that in sprouted black barley, the HRW treatment could change the distribution of phytochemicals (e.g., the ionic strength of guanosine), increase the concentrations of free vanillic acid, coumaric acid, sinapic acid, conjugated sinapic acid, Ca and Fe and the hydroxyl radical scavenging rate, and decrease the protein, fat, starch and dietary fibre contents compared with the results obtained after treatment with ultra-pure water (P < 0.05).
31279124	3	32	theme	hydroxyl	798:805	arg1	rate					826:829	the hydroxyl radical scavenging rate	794:829	the hydroxyl radical scavenging rate	794:829	A chemical component analysis showed that in sprouted black barley, the HRW treatment could change the distribution of phytochemicals (e.g., the ionic strength of guanosine), increase the concentrations of free vanillic acid, coumaric acid, sinapic acid, conjugated sinapic acid, Ca and Fe and the hydroxyl radical scavenging rate, and decrease the protein, fat, starch and dietary fibre contents compared with the results obtained after treatment with ultra-pure water (P < 0.05).
31279124	1	33	theme	increasing	189:198	arg1	attention					200:208	increasing attention	189:208	increasing attention	189:208	Hydrogen gas (H2), a multifunctional signaling molecule, has received increasing attention in recent years.
31279124	0	34	theme	antioxidative	63:75	arg1	characteristics					77:91	antioxidative characteristics	63:91	antioxidative characteristics	63:91	Effects of hydrogen-rich water on the nutrient composition and antioxidative characteristics of sprouted black barley.
31279124	3	35	theme	HRW	572:574	arg1	treatment					576:584	the HRW treatment	568:584	the HRW treatment	568:584	A chemical component analysis showed that in sprouted black barley, the HRW treatment could change the distribution of phytochemicals (e.g., the ionic strength of guanosine), increase the concentrations of free vanillic acid, coumaric acid, sinapic acid, conjugated sinapic acid, Ca and Fe and the hydroxyl radical scavenging rate, and decrease the protein, fat, starch and dietary fibre contents compared with the results obtained after treatment with ultra-pure water (P < 0.05).
31279124	0	36	dep	composition	47:57	arg1	the					34:36	the	34:36	the	34:36	Effects of hydrogen-rich water on the nutrient composition and antioxidative characteristics of sprouted black barley.
31279124	3	37	theme	radical	807:813	arg1	rate					826:829	the hydroxyl radical scavenging rate	794:829	the hydroxyl radical scavenging rate	794:829	A chemical component analysis showed that in sprouted black barley, the HRW treatment could change the distribution of phytochemicals (e.g., the ionic strength of guanosine), increase the concentrations of free vanillic acid, coumaric acid, sinapic acid, conjugated sinapic acid, Ca and Fe and the hydroxyl radical scavenging rate, and decrease the protein, fat, starch and dietary fibre contents compared with the results obtained after treatment with ultra-pure water (P < 0.05).
31279124	2	38	theme	HRW	392:394	arg1	treatment					396:404	the HRW treatment	388:404	the HRW treatment	388:404	In the present study, hydrogen-rich water (HRW) (2 ppm) was used for the processing of sprouted black barley (Hordeum distichum L.), and the results showed that the HRW treatment could significantly increase the germination rate and growth rate of black barley (P < 0.05).
31279124	3	39	theme	vanillic	711:718	arg1	acid					720:723	free vanillic acid	706:723	free vanillic acid	706:723	A chemical component analysis showed that in sprouted black barley, the HRW treatment could change the distribution of phytochemicals (e.g., the ionic strength of guanosine), increase the concentrations of free vanillic acid, coumaric acid, sinapic acid, conjugated sinapic acid, Ca and Fe and the hydroxyl radical scavenging rate, and decrease the protein, fat, starch and dietary fibre contents compared with the results obtained after treatment with ultra-pure water (P < 0.05).
31279124	3	40	theme	chemical	502:509	arg1	analysis					521:528	A chemical component analysis	500:528	A chemical component analysis	500:528	A chemical component analysis showed that in sprouted black barley, the HRW treatment could change the distribution of phytochemicals (e.g., the ionic strength of guanosine), increase the concentrations of free vanillic acid, coumaric acid, sinapic acid, conjugated sinapic acid, Ca and Fe and the hydroxyl radical scavenging rate, and decrease the protein, fat, starch and dietary fibre contents compared with the results obtained after treatment with ultra-pure water (P < 0.05).
31279124	4	41	theme	grains	1029:1034	arg1	processing					1006:1015	the processing	1002:1015	the processing of sprouted grains	1002:1034	HRW can be used for the processing of sprouted grains to effectively increase their germination efficiency and concentrations of bioactive phytochemicals.
31279124	1	42	theme	Hydrogen	119:126	arg1	H2					133:134	H2	133:134	H2	133:134	Hydrogen gas (H2), a multifunctional signaling molecule, has received increasing attention in recent years.
31279124	1	42	theme	Hydrogen	119:126	arg1	gas					128:130	Hydrogen gas	119:130	Hydrogen gas (H2)	119:135	Hydrogen gas (H2), a multifunctional signaling molecule, has received increasing attention in recent years.
31279124	1	42	theme	Hydrogen	119:126	arg1	molecule					166:173	a multifunctional signaling molecule	138:173	a multifunctional signaling molecule	138:173	Hydrogen gas (H2), a multifunctional signaling molecule, has received increasing attention in recent years.
31279124	0	43	theme	nutrient	38:45	arg1	composition					47:57	nutrient composition	38:57	nutrient composition	38:57	Effects of hydrogen-rich water on the nutrient composition and antioxidative characteristics of sprouted black barley.
31279124	3	44	theme	component	511:519	arg1	analysis					521:528	A chemical component analysis	500:528	A chemical component analysis	500:528	A chemical component analysis showed that in sprouted black barley, the HRW treatment could change the distribution of phytochemicals (e.g., the ionic strength of guanosine), increase the concentrations of free vanillic acid, coumaric acid, sinapic acid, conjugated sinapic acid, Ca and Fe and the hydroxyl radical scavenging rate, and decrease the protein, fat, starch and dietary fibre contents compared with the results obtained after treatment with ultra-pure water (P < 0.05).
31279124	3	45	theme	sprouted	545:552	arg1	barley					560:565	sprouted black barley	545:565	sprouted black barley	545:565	A chemical component analysis showed that in sprouted black barley, the HRW treatment could change the distribution of phytochemicals (e.g., the ionic strength of guanosine), increase the concentrations of free vanillic acid, coumaric acid, sinapic acid, conjugated sinapic acid, Ca and Fe and the hydroxyl radical scavenging rate, and decrease the protein, fat, starch and dietary fibre contents compared with the results obtained after treatment with ultra-pure water (P < 0.05).
31279124	3	46	theme	conjugated	755:764	arg1	acid					774:777	conjugated sinapic acid	755:777	conjugated sinapic acid	755:777	A chemical component analysis showed that in sprouted black barley, the HRW treatment could change the distribution of phytochemicals (e.g., the ionic strength of guanosine), increase the concentrations of free vanillic acid, coumaric acid, sinapic acid, conjugated sinapic acid, Ca and Fe and the hydroxyl radical scavenging rate, and decrease the protein, fat, starch and dietary fibre contents compared with the results obtained after treatment with ultra-pure water (P < 0.05).
31279124	1	47	theme	recent	213:218	arg1	years					220:224	recent years	213:224	recent years	213:224	Hydrogen gas (H2), a multifunctional signaling molecule, has received increasing attention in recent years.
31279124	3	48	theme	black	554:558	arg1	barley					560:565	sprouted black barley	545:565	sprouted black barley	545:565	A chemical component analysis showed that in sprouted black barley, the HRW treatment could change the distribution of phytochemicals (e.g., the ionic strength of guanosine), increase the concentrations of free vanillic acid, coumaric acid, sinapic acid, conjugated sinapic acid, Ca and Fe and the hydroxyl radical scavenging rate, and decrease the protein, fat, starch and dietary fibre contents compared with the results obtained after treatment with ultra-pure water (P < 0.05).
31279124	3	49	theme	sinapic	741:747	arg1	acid					749:752	sinapic acid	741:752	sinapic acid	741:752	A chemical component analysis showed that in sprouted black barley, the HRW treatment could change the distribution of phytochemicals (e.g., the ionic strength of guanosine), increase the concentrations of free vanillic acid, coumaric acid, sinapic acid, conjugated sinapic acid, Ca and Fe and the hydroxyl radical scavenging rate, and decrease the protein, fat, starch and dietary fibre contents compared with the results obtained after treatment with ultra-pure water (P < 0.05).
31279124	3	50	theme	acid	720:723	arg1	rate					826:829	the hydroxyl radical scavenging rate	794:829	the hydroxyl radical scavenging rate	794:829	A chemical component analysis showed that in sprouted black barley, the HRW treatment could change the distribution of phytochemicals (e.g., the ionic strength of guanosine), increase the concentrations of free vanillic acid, coumaric acid, sinapic acid, conjugated sinapic acid, Ca and Fe and the hydroxyl radical scavenging rate, and decrease the protein, fat, starch and dietary fibre contents compared with the results obtained after treatment with ultra-pure water (P < 0.05).
31279124	3	50	theme	acid	720:723	arg1	concentrations					688:701	the concentrations	684:701	the concentrations of free vanillic acid, coumaric acid, sinapic acid, conjugated sinapic acid, Ca and Fe	684:788	A chemical component analysis showed that in sprouted black barley, the HRW treatment could change the distribution of phytochemicals (e.g., the ionic strength of guanosine), increase the concentrations of free vanillic acid, coumaric acid, sinapic acid, conjugated sinapic acid, Ca and Fe and the hydroxyl radical scavenging rate, and decrease the protein, fat, starch and dietary fibre contents compared with the results obtained after treatment with ultra-pure water (P < 0.05).
31279124	2	51	theme	germination	439:449	arg1	rate					451:454	germination rate	439:454	germination rate	439:454	In the present study, hydrogen-rich water (HRW) (2 ppm) was used for the processing of sprouted black barley (Hordeum distichum L.), and the results showed that the HRW treatment could significantly increase the germination rate and growth rate of black barley (P < 0.05).
31279124	2	52	theme	present	234:240	arg1	study					242:246	the present study	230:246	the present study	230:246	In the present study, hydrogen-rich water (HRW) (2 ppm) was used for the processing of sprouted black barley (Hordeum distichum L.), and the results showed that the HRW treatment could significantly increase the germination rate and growth rate of black barley (P < 0.05).
31279124	2	53	theme	black	323:327	arg1	barley					329:334	sprouted black barley	314:334	sprouted black barley (Hordeum distichum L.)	314:357	In the present study, hydrogen-rich water (HRW) (2 ppm) was used for the processing of sprouted black barley (Hordeum distichum L.), and the results showed that the HRW treatment could significantly increase the germination rate and growth rate of black barley (P < 0.05).
31279124	2	53	theme	black	323:327	arg1	L.					355:356	L.	355:356	L.	355:356	In the present study, hydrogen-rich water (HRW) (2 ppm) was used for the processing of sprouted black barley (Hordeum distichum L.), and the results showed that the HRW treatment could significantly increase the germination rate and growth rate of black barley (P < 0.05).
31279124	3	54	theme	acid	749:752	arg1	rate					826:829	the hydroxyl radical scavenging rate	794:829	the hydroxyl radical scavenging rate	794:829	A chemical component analysis showed that in sprouted black barley, the HRW treatment could change the distribution of phytochemicals (e.g., the ionic strength of guanosine), increase the concentrations of free vanillic acid, coumaric acid, sinapic acid, conjugated sinapic acid, Ca and Fe and the hydroxyl radical scavenging rate, and decrease the protein, fat, starch and dietary fibre contents compared with the results obtained after treatment with ultra-pure water (P < 0.05).
31279124	3	54	theme	acid	749:752	arg1	concentrations					688:701	the concentrations	684:701	the concentrations of free vanillic acid, coumaric acid, sinapic acid, conjugated sinapic acid, Ca and Fe	684:788	A chemical component analysis showed that in sprouted black barley, the HRW treatment could change the distribution of phytochemicals (e.g., the ionic strength of guanosine), increase the concentrations of free vanillic acid, coumaric acid, sinapic acid, conjugated sinapic acid, Ca and Fe and the hydroxyl radical scavenging rate, and decrease the protein, fat, starch and dietary fibre contents compared with the results obtained after treatment with ultra-pure water (P < 0.05).
31279124	3	55	dep	strength	651:658	arg1	e.g.					635:638	e.g.	635:638	e.g.	635:638	A chemical component analysis showed that in sprouted black barley, the HRW treatment could change the distribution of phytochemicals (e.g., the ionic strength of guanosine), increase the concentrations of free vanillic acid, coumaric acid, sinapic acid, conjugated sinapic acid, Ca and Fe and the hydroxyl radical scavenging rate, and decrease the protein, fat, starch and dietary fibre contents compared with the results obtained after treatment with ultra-pure water (P < 0.05).
31279124	3	56	theme	free	706:709	arg1	acid					720:723	free vanillic acid	706:723	free vanillic acid	706:723	A chemical component analysis showed that in sprouted black barley, the HRW treatment could change the distribution of phytochemicals (e.g., the ionic strength of guanosine), increase the concentrations of free vanillic acid, coumaric acid, sinapic acid, conjugated sinapic acid, Ca and Fe and the hydroxyl radical scavenging rate, and decrease the protein, fat, starch and dietary fibre contents compared with the results obtained after treatment with ultra-pure water (P < 0.05).
31279124	4	57	theme	sprouted	1020:1027	arg1	grains					1029:1034	sprouted grains	1020:1034	sprouted grains	1020:1034	HRW can be used for the processing of sprouted grains to effectively increase their germination efficiency and concentrations of bioactive phytochemicals.
31279124	2	58	theme	sprouted	314:321	arg1	barley					329:334	sprouted black barley	314:334	sprouted black barley (Hordeum distichum L.)	314:357	In the present study, hydrogen-rich water (HRW) (2 ppm) was used for the processing of sprouted black barley (Hordeum distichum L.), and the results showed that the HRW treatment could significantly increase the germination rate and growth rate of black barley (P < 0.05).
31279124	2	58	theme	sprouted	314:321	arg1	L.					355:356	L.	355:356	L.	355:356	In the present study, hydrogen-rich water (HRW) (2 ppm) was used for the processing of sprouted black barley (Hordeum distichum L.), and the results showed that the HRW treatment could significantly increase the germination rate and growth rate of black barley (P < 0.05).
31279124	0	59	theme	sprouted	96:103	arg1	barley					111:116	sprouted black barley	96:116	sprouted black barley	96:116	Effects of hydrogen-rich water on the nutrient composition and antioxidative characteristics of sprouted black barley.
31176647	2	0	theme	material	366:373	arg1	SDS					399:401	SDS	399:401	SDS	399:401	To solve the problem, virus-mimicking nanosized polyelectrolyte complex (PEC) was designed and their capacity in enhancing peroral insulin absorption in combination with bifunctional material sodium dodecyl sulfate (SDS) coating was investigated.
31176647	2	0	theme	material	366:373	arg1	sulfate					390:396	material sodium dodecyl sulfate	366:396	bifunctional material sodium dodecyl sulfate (SDS) coating	353:410	To solve the problem, virus-mimicking nanosized polyelectrolyte complex (PEC) was designed and their capacity in enhancing peroral insulin absorption in combination with bifunctional material sodium dodecyl sulfate (SDS) coating was investigated.
31176647	5	1	from	coating	1083:1089	arg1	surface					1098:1104	the surface	1094:1104	the surface of virus-mimicking PECs	1094:1128	Thereafter, SDS solution was applied as outer layer coating on the surface of virus-mimicking PECs.
31176647	3	2	theme	envelope	702:709	arg1	structure					656:664	structure	656:664	structure	656:664	Inspired by nature, virus-mimicking chitosan (CS)-modified L-Phe derivatives were synthesized to simulate the components of viral envelopes and then PECs between CS-g-N-Phe copolymers and insulin were prepared to achieve both structure and composition simulation of virus envelope.
31176647	3	2	theme	envelope	702:709	arg1	simulation					682:691	composition simulation	670:691	composition simulation of virus envelope	670:709	Inspired by nature, virus-mimicking chitosan (CS)-modified L-Phe derivatives were synthesized to simulate the components of viral envelopes and then PECs between CS-g-N-Phe copolymers and insulin were prepared to achieve both structure and composition simulation of virus envelope.
31176647	1	3	theme	oral	160:163	arg1	delivery					173:180	oral insulin delivery	160:180	oral insulin delivery	160:180	The viscous and elastic mucus layer is still an undesirable barrier for oral insulin delivery.
31176647	4	4	theme	pharmacological	965:979	arg1	bioavailability					981:995	2.2-fold higher relative pharmacological bioavailability	940:995	2.2-fold higher relative pharmacological bioavailability than nonmodified CS/insulin PECs	940:1028	Based on the results from both in vitro and in vivo studies, it was concluded that in vitro mucodiffusion and in vivo hypoglycemic effect were dependent on L-Phe graft ratio, with CS-g-N-Phe20.2%/insulin PECs presenting 2.0- to 2.2-fold higher relative pharmacological bioavailability than nonmodified CS/insulin PECs.
31176647	2	5	theme	bifunctional	353:364	arg1	coating					404:410	bifunctional material sodium dodecyl sulfate (SDS) coating	353:410	bifunctional material sodium dodecyl sulfate (SDS) coating	353:410	To solve the problem, virus-mimicking nanosized polyelectrolyte complex (PEC) was designed and their capacity in enhancing peroral insulin absorption in combination with bifunctional material sodium dodecyl sulfate (SDS) coating was investigated.
31176647	6	6	theme	SDS	1261:1263	arg1	manner					1289:1294	an SDS concentration-dependent manner	1258:1294	an SDS concentration-dependent manner	1258:1294	The coated PECs showed improved enzymatic stability, enhanced transport across mucus layer as well as intestinal epithelium in an SDS concentration-dependent manner, with 0.6% SDS coating presenting the best effect, with further enhanced relative pharmacological bioavailability in healthy rats and prolonged therapeutic effect up to 9 h.
31176647	4	7	with	dependent	855:863	arg1	PECs					916:919	CS-g-N-Phe20.2%/insulin PECs	892:919	CS-g-N-Phe20.2%/insulin PECs presenting 2.0- to 2.2-fold higher relative pharmacological bioavailability than nonmodified CS/insulin PECs	892:1028	Based on the results from both in vitro and in vivo studies, it was concluded that in vitro mucodiffusion and in vivo hypoglycemic effect were dependent on L-Phe graft ratio, with CS-g-N-Phe20.2%/insulin PECs presenting 2.0- to 2.2-fold higher relative pharmacological bioavailability than nonmodified CS/insulin PECs.
31176647	2	8	theme	insulin	314:320	arg1	absorption					322:331	peroral insulin absorption	306:331	peroral insulin absorption	306:331	To solve the problem, virus-mimicking nanosized polyelectrolyte complex (PEC) was designed and their capacity in enhancing peroral insulin absorption in combination with bifunctional material sodium dodecyl sulfate (SDS) coating was investigated.
31176647	6	9	theme	SDS	1307:1309	arg1	coating					1311:1317	0.6% SDS coating	1302:1317	0.6% SDS coating presenting the best effect	1302:1344	The coated PECs showed improved enzymatic stability, enhanced transport across mucus layer as well as intestinal epithelium in an SDS concentration-dependent manner, with 0.6% SDS coating presenting the best effect, with further enhanced relative pharmacological bioavailability in healthy rats and prolonged therapeutic effect up to 9 h.
31176647	3	10	theme	viral	554:558	arg1	envelopes					560:568	viral envelopes	554:568	viral envelopes	554:568	Inspired by nature, virus-mimicking chitosan (CS)-modified L-Phe derivatives were synthesized to simulate the components of viral envelopes and then PECs between CS-g-N-Phe copolymers and insulin were prepared to achieve both structure and composition simulation of virus envelope.
31176647	6	11	theme	pharmacological	1378:1392	arg1	bioavailability					1394:1408	relative pharmacological bioavailability	1369:1408	relative pharmacological bioavailability in healthy rats	1369:1424	The coated PECs showed improved enzymatic stability, enhanced transport across mucus layer as well as intestinal epithelium in an SDS concentration-dependent manner, with 0.6% SDS coating presenting the best effect, with further enhanced relative pharmacological bioavailability in healthy rats and prolonged therapeutic effect up to 9 h.
31176647	4	12	dep	studies	764:770	arg1	in vivo					756:762	in vivo	756:762	in vivo	756:762	Based on the results from both in vitro and in vivo studies, it was concluded that in vitro mucodiffusion and in vivo hypoglycemic effect were dependent on L-Phe graft ratio, with CS-g-N-Phe20.2%/insulin PECs presenting 2.0- to 2.2-fold higher relative pharmacological bioavailability than nonmodified CS/insulin PECs.
31176647	4	12	dep	studies	764:770	arg1	both					738:741	both	738:741	both	738:741	Based on the results from both in vitro and in vivo studies, it was concluded that in vitro mucodiffusion and in vivo hypoglycemic effect were dependent on L-Phe graft ratio, with CS-g-N-Phe20.2%/insulin PECs presenting 2.0- to 2.2-fold higher relative pharmacological bioavailability than nonmodified CS/insulin PECs.
31176647	6	13	theme	%	1305:1305	arg1	coating					1311:1317	0.6% SDS coating	1302:1317	0.6% SDS coating presenting the best effect	1302:1344	The coated PECs showed improved enzymatic stability, enhanced transport across mucus layer as well as intestinal epithelium in an SDS concentration-dependent manner, with 0.6% SDS coating presenting the best effect, with further enhanced relative pharmacological bioavailability in healthy rats and prolonged therapeutic effect up to 9 h.
31176647	1	14	theme	insulin	165:171	arg1	delivery					173:180	oral insulin delivery	160:180	oral insulin delivery	160:180	The viscous and elastic mucus layer is still an undesirable barrier for oral insulin delivery.
31176647	6	15	theme	0.6	1302:1304	arg1	%					1305:1305	%	1305:1305	%	1305:1305	The coated PECs showed improved enzymatic stability, enhanced transport across mucus layer as well as intestinal epithelium in an SDS concentration-dependent manner, with 0.6% SDS coating presenting the best effect, with further enhanced relative pharmacological bioavailability in healthy rats and prolonged therapeutic effect up to 9 h.
31176647	4	16	from	studies	764:770	arg1	results					725:731	the results	721:731	the results from both in vitro and in vivo studies	721:770	Based on the results from both in vitro and in vivo studies, it was concluded that in vitro mucodiffusion and in vivo hypoglycemic effect were dependent on L-Phe graft ratio, with CS-g-N-Phe20.2%/insulin PECs presenting 2.0- to 2.2-fold higher relative pharmacological bioavailability than nonmodified CS/insulin PECs.
31176647	6	17	theme	mucus	1210:1214	arg1	layer					1216:1220	mucus layer	1210:1220	mucus layer as well as intestinal epithelium	1210:1253	The coated PECs showed improved enzymatic stability, enhanced transport across mucus layer as well as intestinal epithelium in an SDS concentration-dependent manner, with 0.6% SDS coating presenting the best effect, with further enhanced relative pharmacological bioavailability in healthy rats and prolonged therapeutic effect up to 9 h.
31176647	4	18	theme	nonmodified	1002:1012	arg1	PECs					1025:1028	nonmodified CS/insulin PECs	1002:1028	nonmodified CS/insulin PECs	1002:1028	Based on the results from both in vitro and in vivo studies, it was concluded that in vitro mucodiffusion and in vivo hypoglycemic effect were dependent on L-Phe graft ratio, with CS-g-N-Phe20.2%/insulin PECs presenting 2.0- to 2.2-fold higher relative pharmacological bioavailability than nonmodified CS/insulin PECs.
31176647	4	19	theme	graft	874:878	arg1	ratio					880:884	L-Phe graft ratio	868:884	L-Phe graft ratio	868:884	Based on the results from both in vitro and in vivo studies, it was concluded that in vitro mucodiffusion and in vivo hypoglycemic effect were dependent on L-Phe graft ratio, with CS-g-N-Phe20.2%/insulin PECs presenting 2.0- to 2.2-fold higher relative pharmacological bioavailability than nonmodified CS/insulin PECs.
31176647	1	20	theme	viscous	92:98	arg1	barrier					148:154	an undesirable barrier	133:154	an undesirable barrier for oral insulin delivery	133:180	The viscous and elastic mucus layer is still an undesirable barrier for oral insulin delivery.
31176647	1	20	theme	viscous	92:98	arg1	layer					118:122	The viscous and elastic mucus layer	88:122	The viscous and elastic mucus layer	88:122	The viscous and elastic mucus layer is still an undesirable barrier for oral insulin delivery.
31176647	6	21	from	bioavailability	1394:1408	arg1	rats					1421:1424	healthy rats	1413:1424	healthy rats	1413:1424	The coated PECs showed improved enzymatic stability, enhanced transport across mucus layer as well as intestinal epithelium in an SDS concentration-dependent manner, with 0.6% SDS coating presenting the best effect, with further enhanced relative pharmacological bioavailability in healthy rats and prolonged therapeutic effect up to 9 h.
31176647	6	22	dep	h	1467:1467	arg1	up					1459:1460	up	1459:1460	up	1459:1460	The coated PECs showed improved enzymatic stability, enhanced transport across mucus layer as well as intestinal epithelium in an SDS concentration-dependent manner, with 0.6% SDS coating presenting the best effect, with further enhanced relative pharmacological bioavailability in healthy rats and prolonged therapeutic effect up to 9 h.
31176647	4	23	theme	L-Phe	868:872	arg1	ratio					880:884	L-Phe graft ratio	868:884	L-Phe graft ratio	868:884	Based on the results from both in vitro and in vivo studies, it was concluded that in vitro mucodiffusion and in vivo hypoglycemic effect were dependent on L-Phe graft ratio, with CS-g-N-Phe20.2%/insulin PECs presenting 2.0- to 2.2-fold higher relative pharmacological bioavailability than nonmodified CS/insulin PECs.
31176647	2	24	theme	peroral	306:312	arg1	absorption					322:331	peroral insulin absorption	306:331	peroral insulin absorption	306:331	To solve the problem, virus-mimicking nanosized polyelectrolyte complex (PEC) was designed and their capacity in enhancing peroral insulin absorption in combination with bifunctional material sodium dodecyl sulfate (SDS) coating was investigated.
31176647	2	25	theme	sulfate	390:396	arg1	coating					404:410	bifunctional material sodium dodecyl sulfate (SDS) coating	353:410	bifunctional material sodium dodecyl sulfate (SDS) coating	353:410	To solve the problem, virus-mimicking nanosized polyelectrolyte complex (PEC) was designed and their capacity in enhancing peroral insulin absorption in combination with bifunctional material sodium dodecyl sulfate (SDS) coating was investigated.
31176647	0	26	theme	Polyelectrolyte	26:40	arg1	Complexes					42:50	Virus-Mimicking Polyelectrolyte Complexes	10:50	Virus-Mimicking Polyelectrolyte Complexes	10:50	Design of Virus-Mimicking Polyelectrolyte Complexes for Enhanced Oral Insulin Delivery.
31176647	6	27	theme	intestinal	1233:1242	arg1	epithelium					1244:1253	intestinal epithelium	1233:1253	mucus layer as well as intestinal epithelium	1210:1253	The coated PECs showed improved enzymatic stability, enhanced transport across mucus layer as well as intestinal epithelium in an SDS concentration-dependent manner, with 0.6% SDS coating presenting the best effect, with further enhanced relative pharmacological bioavailability in healthy rats and prolonged therapeutic effect up to 9 h.
31176647	5	28	theme	PECs	1125:1128	arg1	surface					1098:1104	the surface	1094:1104	the surface of virus-mimicking PECs	1094:1128	Thereafter, SDS solution was applied as outer layer coating on the surface of virus-mimicking PECs.
31176647	3	29	theme	CS-g-N-Phe	592:601	arg1	copolymers					603:612	CS-g-N-Phe copolymers	592:612	CS-g-N-Phe copolymers	592:612	Inspired by nature, virus-mimicking chitosan (CS)-modified L-Phe derivatives were synthesized to simulate the components of viral envelopes and then PECs between CS-g-N-Phe copolymers and insulin were prepared to achieve both structure and composition simulation of virus envelope.
31176647	1	30	theme	elastic	104:110	arg1	barrier					148:154	an undesirable barrier	133:154	an undesirable barrier for oral insulin delivery	133:180	The viscous and elastic mucus layer is still an undesirable barrier for oral insulin delivery.
31176647	1	30	theme	elastic	104:110	arg1	layer					118:122	The viscous and elastic mucus layer	88:122	The viscous and elastic mucus layer	88:122	The viscous and elastic mucus layer is still an undesirable barrier for oral insulin delivery.
31176647	5	31	theme	outer	1071:1075	arg1	solution					1047:1054	SDS solution	1043:1054	SDS solution	1043:1054	Thereafter, SDS solution was applied as outer layer coating on the surface of virus-mimicking PECs.
31176647	5	31	theme	outer	1071:1075	arg1	coating					1083:1089	outer layer coating	1071:1089	outer layer coating on the surface of virus-mimicking PECs	1071:1128	Thereafter, SDS solution was applied as outer layer coating on the surface of virus-mimicking PECs.
31176647	0	32	theme	Virus-Mimicking	10:24	arg1	Complexes					42:50	Virus-Mimicking Polyelectrolyte Complexes	10:50	Virus-Mimicking Polyelectrolyte Complexes	10:50	Design of Virus-Mimicking Polyelectrolyte Complexes for Enhanced Oral Insulin Delivery.
31176647	6	33	theme	relative	1369:1376	arg1	bioavailability					1394:1408	relative pharmacological bioavailability	1369:1408	relative pharmacological bioavailability in healthy rats	1369:1424	The coated PECs showed improved enzymatic stability, enhanced transport across mucus layer as well as intestinal epithelium in an SDS concentration-dependent manner, with 0.6% SDS coating presenting the best effect, with further enhanced relative pharmacological bioavailability in healthy rats and prolonged therapeutic effect up to 9 h.
31176647	1	34	theme	mucus	112:116	arg1	barrier					148:154	an undesirable barrier	133:154	an undesirable barrier for oral insulin delivery	133:180	The viscous and elastic mucus layer is still an undesirable barrier for oral insulin delivery.
31176647	1	34	theme	mucus	112:116	arg1	layer					118:122	The viscous and elastic mucus layer	88:122	The viscous and elastic mucus layer	88:122	The viscous and elastic mucus layer is still an undesirable barrier for oral insulin delivery.
31176647	1	35	theme	undesirable	136:146	arg1	barrier					148:154	an undesirable barrier	133:154	an undesirable barrier for oral insulin delivery	133:180	The viscous and elastic mucus layer is still an undesirable barrier for oral insulin delivery.
31176647	1	35	theme	undesirable	136:146	arg1	layer					118:122	The viscous and elastic mucus layer	88:122	The viscous and elastic mucus layer	88:122	The viscous and elastic mucus layer is still an undesirable barrier for oral insulin delivery.
31176647	6	36	theme	enzymatic	1163:1171	arg1	stability					1173:1181	improved enzymatic stability	1154:1181	improved enzymatic stability	1154:1181	The coated PECs showed improved enzymatic stability, enhanced transport across mucus layer as well as intestinal epithelium in an SDS concentration-dependent manner, with 0.6% SDS coating presenting the best effect, with further enhanced relative pharmacological bioavailability in healthy rats and prolonged therapeutic effect up to 9 h.
31176647	4	37	theme	higher	949:954	arg1	bioavailability					981:995	2.2-fold higher relative pharmacological bioavailability	940:995	2.2-fold higher relative pharmacological bioavailability than nonmodified CS/insulin PECs	940:1028	Based on the results from both in vitro and in vivo studies, it was concluded that in vitro mucodiffusion and in vivo hypoglycemic effect were dependent on L-Phe graft ratio, with CS-g-N-Phe20.2%/insulin PECs presenting 2.0- to 2.2-fold higher relative pharmacological bioavailability than nonmodified CS/insulin PECs.
31176647	4	38	theme	hypoglycemic	830:841	arg1	effect					843:848	in vivo hypoglycemic effect	822:848	in vivo hypoglycemic effect	822:848	Based on the results from both in vitro and in vivo studies, it was concluded that in vitro mucodiffusion and in vivo hypoglycemic effect were dependent on L-Phe graft ratio, with CS-g-N-Phe20.2%/insulin PECs presenting 2.0- to 2.2-fold higher relative pharmacological bioavailability than nonmodified CS/insulin PECs.
31176647	3	39	theme	virus-mimicking	450:464	arg1	chitosan					466:473	virus-mimicking chitosan	450:473	virus-mimicking chitosan (CS)-modified L-Phe derivatives	450:505	Inspired by nature, virus-mimicking chitosan (CS)-modified L-Phe derivatives were synthesized to simulate the components of viral envelopes and then PECs between CS-g-N-Phe copolymers and insulin were prepared to achieve both structure and composition simulation of virus envelope.
31176647	3	39	theme	virus-mimicking	450:464	arg1	CS					476:477	CS	476:477	CS	476:477	Inspired by nature, virus-mimicking chitosan (CS)-modified L-Phe derivatives were synthesized to simulate the components of viral envelopes and then PECs between CS-g-N-Phe copolymers and insulin were prepared to achieve both structure and composition simulation of virus envelope.
31176647	0	40	theme	Complexes	42:50	arg1	Design					0:5	Design	0:5	Design of Virus-Mimicking Polyelectrolyte Complexes for Enhanced Oral Insulin Delivery.	0:86	Design of Virus-Mimicking Polyelectrolyte Complexes for Enhanced Oral Insulin Delivery.
31176647	6	41	theme	therapeutic	1440:1450	arg1	effect					1452:1457	therapeutic effect	1440:1457	therapeutic effect	1440:1457	The coated PECs showed improved enzymatic stability, enhanced transport across mucus layer as well as intestinal epithelium in an SDS concentration-dependent manner, with 0.6% SDS coating presenting the best effect, with further enhanced relative pharmacological bioavailability in healthy rats and prolonged therapeutic effect up to 9 h.
31176647	5	42	theme	virus-mimicking	1109:1123	arg1	PECs					1125:1128	virus-mimicking PECs	1109:1128	virus-mimicking PECs	1109:1128	Thereafter, SDS solution was applied as outer layer coating on the surface of virus-mimicking PECs.
31176647	2	43	theme	polyelectrolyte	231:245	arg1	PEC					256:258	PEC	256:258	PEC	256:258	To solve the problem, virus-mimicking nanosized polyelectrolyte complex (PEC) was designed and their capacity in enhancing peroral insulin absorption in combination with bifunctional material sodium dodecyl sulfate (SDS) coating was investigated.
31176647	2	43	theme	polyelectrolyte	231:245	arg1	complex					247:253	virus-mimicking nanosized polyelectrolyte complex	205:253	virus-mimicking nanosized polyelectrolyte complex (PEC)	205:259	To solve the problem, virus-mimicking nanosized polyelectrolyte complex (PEC) was designed and their capacity in enhancing peroral insulin absorption in combination with bifunctional material sodium dodecyl sulfate (SDS) coating was investigated.
31176647	3	44	theme	chitosan	466:473	arg1	derivatives					495:505	virus-mimicking chitosan (CS)-modified L-Phe derivatives	450:505	virus-mimicking chitosan (CS)-modified L-Phe derivatives	450:505	Inspired by nature, virus-mimicking chitosan (CS)-modified L-Phe derivatives were synthesized to simulate the components of viral envelopes and then PECs between CS-g-N-Phe copolymers and insulin were prepared to achieve both structure and composition simulation of virus envelope.
31176647	0	45	theme	Oral	65:68	arg1	Delivery					78:85	Enhanced Oral Insulin Delivery	56:85	Enhanced Oral Insulin Delivery	56:85	Design of Virus-Mimicking Polyelectrolyte Complexes for Enhanced Oral Insulin Delivery.
31176647	4	46	theme	/insulin	907:914	arg1	PECs					916:919	CS-g-N-Phe20.2%/insulin PECs	892:919	CS-g-N-Phe20.2%/insulin PECs presenting 2.0- to 2.2-fold higher relative pharmacological bioavailability than nonmodified CS/insulin PECs	892:1028	Based on the results from both in vitro and in vivo studies, it was concluded that in vitro mucodiffusion and in vivo hypoglycemic effect were dependent on L-Phe graft ratio, with CS-g-N-Phe20.2%/insulin PECs presenting 2.0- to 2.2-fold higher relative pharmacological bioavailability than nonmodified CS/insulin PECs.
31176647	2	47	with	combination	336:346	arg1	coating					404:410	bifunctional material sodium dodecyl sulfate (SDS) coating	353:410	bifunctional material sodium dodecyl sulfate (SDS) coating	353:410	To solve the problem, virus-mimicking nanosized polyelectrolyte complex (PEC) was designed and their capacity in enhancing peroral insulin absorption in combination with bifunctional material sodium dodecyl sulfate (SDS) coating was investigated.
31176647	2	48	theme	nanosized	221:229	arg1	PEC					256:258	PEC	256:258	PEC	256:258	To solve the problem, virus-mimicking nanosized polyelectrolyte complex (PEC) was designed and their capacity in enhancing peroral insulin absorption in combination with bifunctional material sodium dodecyl sulfate (SDS) coating was investigated.
31176647	2	48	theme	nanosized	221:229	arg1	complex					247:253	virus-mimicking nanosized polyelectrolyte complex	205:253	virus-mimicking nanosized polyelectrolyte complex (PEC)	205:259	To solve the problem, virus-mimicking nanosized polyelectrolyte complex (PEC) was designed and their capacity in enhancing peroral insulin absorption in combination with bifunctional material sodium dodecyl sulfate (SDS) coating was investigated.
31176647	3	49	theme	L-Phe	489:493	arg1	derivatives					495:505	virus-mimicking chitosan (CS)-modified L-Phe derivatives	450:505	virus-mimicking chitosan (CS)-modified L-Phe derivatives	450:505	Inspired by nature, virus-mimicking chitosan (CS)-modified L-Phe derivatives were synthesized to simulate the components of viral envelopes and then PECs between CS-g-N-Phe copolymers and insulin were prepared to achieve both structure and composition simulation of virus envelope.
31176647	0	50	theme	Enhanced	56:63	arg1	Delivery					78:85	Enhanced Oral Insulin Delivery	56:85	Enhanced Oral Insulin Delivery	56:85	Design of Virus-Mimicking Polyelectrolyte Complexes for Enhanced Oral Insulin Delivery.
31176647	6	51	theme	concentration-dependent	1265:1287	arg1	manner					1289:1294	an SDS concentration-dependent manner	1258:1294	an SDS concentration-dependent manner	1258:1294	The coated PECs showed improved enzymatic stability, enhanced transport across mucus layer as well as intestinal epithelium in an SDS concentration-dependent manner, with 0.6% SDS coating presenting the best effect, with further enhanced relative pharmacological bioavailability in healthy rats and prolonged therapeutic effect up to 9 h.
31176647	4	52	theme	%	906:906	arg1	PECs					916:919	CS-g-N-Phe20.2%/insulin PECs	892:919	CS-g-N-Phe20.2%/insulin PECs presenting 2.0- to 2.2-fold higher relative pharmacological bioavailability than nonmodified CS/insulin PECs	892:1028	Based on the results from both in vitro and in vivo studies, it was concluded that in vitro mucodiffusion and in vivo hypoglycemic effect were dependent on L-Phe graft ratio, with CS-g-N-Phe20.2%/insulin PECs presenting 2.0- to 2.2-fold higher relative pharmacological bioavailability than nonmodified CS/insulin PECs.
31176647	5	53	theme	layer	1077:1081	arg1	solution					1047:1054	SDS solution	1043:1054	SDS solution	1043:1054	Thereafter, SDS solution was applied as outer layer coating on the surface of virus-mimicking PECs.
31176647	5	53	theme	layer	1077:1081	arg1	coating					1083:1089	outer layer coating	1071:1089	outer layer coating on the surface of virus-mimicking PECs	1071:1128	Thereafter, SDS solution was applied as outer layer coating on the surface of virus-mimicking PECs.
31176647	2	54	theme	virus-mimicking	205:219	arg1	PEC					256:258	PEC	256:258	PEC	256:258	To solve the problem, virus-mimicking nanosized polyelectrolyte complex (PEC) was designed and their capacity in enhancing peroral insulin absorption in combination with bifunctional material sodium dodecyl sulfate (SDS) coating was investigated.
31176647	2	54	theme	virus-mimicking	205:219	arg1	complex					247:253	virus-mimicking nanosized polyelectrolyte complex	205:253	virus-mimicking nanosized polyelectrolyte complex (PEC)	205:259	To solve the problem, virus-mimicking nanosized polyelectrolyte complex (PEC) was designed and their capacity in enhancing peroral insulin absorption in combination with bifunctional material sodium dodecyl sulfate (SDS) coating was investigated.
31176647	4	55	theme	CS-g-N-Phe20.2	892:905	arg1	%					906:906	%	906:906	%	906:906	Based on the results from both in vitro and in vivo studies, it was concluded that in vitro mucodiffusion and in vivo hypoglycemic effect were dependent on L-Phe graft ratio, with CS-g-N-Phe20.2%/insulin PECs presenting 2.0- to 2.2-fold higher relative pharmacological bioavailability than nonmodified CS/insulin PECs.
31176647	3	56	theme	virus	696:700	arg1	envelope					702:709	virus envelope	696:709	virus envelope	696:709	Inspired by nature, virus-mimicking chitosan (CS)-modified L-Phe derivatives were synthesized to simulate the components of viral envelopes and then PECs between CS-g-N-Phe copolymers and insulin were prepared to achieve both structure and composition simulation of virus envelope.
31176647	4	57	theme	in vitro	795:802	arg1	mucodiffusion					804:816	in vitro mucodiffusion	795:816	in vitro mucodiffusion	795:816	Based on the results from both in vitro and in vivo studies, it was concluded that in vitro mucodiffusion and in vivo hypoglycemic effect were dependent on L-Phe graft ratio, with CS-g-N-Phe20.2%/insulin PECs presenting 2.0- to 2.2-fold higher relative pharmacological bioavailability than nonmodified CS/insulin PECs.
31176647	4	58	theme	in vivo	822:828	arg1	effect					843:848	in vivo hypoglycemic effect	822:848	in vivo hypoglycemic effect	822:848	Based on the results from both in vitro and in vivo studies, it was concluded that in vitro mucodiffusion and in vivo hypoglycemic effect were dependent on L-Phe graft ratio, with CS-g-N-Phe20.2%/insulin PECs presenting 2.0- to 2.2-fold higher relative pharmacological bioavailability than nonmodified CS/insulin PECs.
31176647	6	59	theme	improved	1154:1161	arg1	stability					1173:1181	improved enzymatic stability	1154:1181	improved enzymatic stability	1154:1181	The coated PECs showed improved enzymatic stability, enhanced transport across mucus layer as well as intestinal epithelium in an SDS concentration-dependent manner, with 0.6% SDS coating presenting the best effect, with further enhanced relative pharmacological bioavailability in healthy rats and prolonged therapeutic effect up to 9 h.
31176647	3	60	theme	envelopes	560:568	arg1	components					540:549	the components	536:549	the components of viral envelopes	536:568	Inspired by nature, virus-mimicking chitosan (CS)-modified L-Phe derivatives were synthesized to simulate the components of viral envelopes and then PECs between CS-g-N-Phe copolymers and insulin were prepared to achieve both structure and composition simulation of virus envelope.
31176647	4	61	dep	both	738:741	arg1	in vitro					743:750	in vitro	743:750	in vitro	743:750	Based on the results from both in vitro and in vivo studies, it was concluded that in vitro mucodiffusion and in vivo hypoglycemic effect were dependent on L-Phe graft ratio, with CS-g-N-Phe20.2%/insulin PECs presenting 2.0- to 2.2-fold higher relative pharmacological bioavailability than nonmodified CS/insulin PECs.
31176647	6	62	theme	best	1334:1337	arg1	effect					1339:1344	the best effect	1330:1344	the best effect	1330:1344	The coated PECs showed improved enzymatic stability, enhanced transport across mucus layer as well as intestinal epithelium in an SDS concentration-dependent manner, with 0.6% SDS coating presenting the best effect, with further enhanced relative pharmacological bioavailability in healthy rats and prolonged therapeutic effect up to 9 h.
31176647	3	63	theme	composition	670:680	arg1	simulation					682:691	composition simulation	670:691	composition simulation of virus envelope	670:709	Inspired by nature, virus-mimicking chitosan (CS)-modified L-Phe derivatives were synthesized to simulate the components of viral envelopes and then PECs between CS-g-N-Phe copolymers and insulin were prepared to achieve both structure and composition simulation of virus envelope.
31176647	3	64	theme	-modified	479:487	arg1	derivatives					495:505	virus-mimicking chitosan (CS)-modified L-Phe derivatives	450:505	virus-mimicking chitosan (CS)-modified L-Phe derivatives	450:505	Inspired by nature, virus-mimicking chitosan (CS)-modified L-Phe derivatives were synthesized to simulate the components of viral envelopes and then PECs between CS-g-N-Phe copolymers and insulin were prepared to achieve both structure and composition simulation of virus envelope.
31176647	6	65	theme	healthy	1413:1419	arg1	rats					1421:1424	healthy rats	1413:1424	healthy rats	1413:1424	The coated PECs showed improved enzymatic stability, enhanced transport across mucus layer as well as intestinal epithelium in an SDS concentration-dependent manner, with 0.6% SDS coating presenting the best effect, with further enhanced relative pharmacological bioavailability in healthy rats and prolonged therapeutic effect up to 9 h.
31176647	4	66	theme	CS/insulin	1014:1023	arg1	PECs					1025:1028	nonmodified CS/insulin PECs	1002:1028	nonmodified CS/insulin PECs	1002:1028	Based on the results from both in vitro and in vivo studies, it was concluded that in vitro mucodiffusion and in vivo hypoglycemic effect were dependent on L-Phe graft ratio, with CS-g-N-Phe20.2%/insulin PECs presenting 2.0- to 2.2-fold higher relative pharmacological bioavailability than nonmodified CS/insulin PECs.
31176647	6	67	theme	coated	1135:1140	arg1	PECs					1142:1145	The coated PECs	1131:1145	The coated PECs	1131:1145	The coated PECs showed improved enzymatic stability, enhanced transport across mucus layer as well as intestinal epithelium in an SDS concentration-dependent manner, with 0.6% SDS coating presenting the best effect, with further enhanced relative pharmacological bioavailability in healthy rats and prolonged therapeutic effect up to 9 h.
31176647	5	68	theme	SDS	1043:1045	arg1	solution					1047:1054	SDS solution	1043:1054	SDS solution	1043:1054	Thereafter, SDS solution was applied as outer layer coating on the surface of virus-mimicking PECs.
31176647	5	68	theme	SDS	1043:1045	arg1	coating					1083:1089	outer layer coating	1071:1089	outer layer coating on the surface of virus-mimicking PECs	1071:1128	Thereafter, SDS solution was applied as outer layer coating on the surface of virus-mimicking PECs.
31176647	2	69	theme	dodecyl	382:388	arg1	SDS					399:401	SDS	399:401	SDS	399:401	To solve the problem, virus-mimicking nanosized polyelectrolyte complex (PEC) was designed and their capacity in enhancing peroral insulin absorption in combination with bifunctional material sodium dodecyl sulfate (SDS) coating was investigated.
31176647	2	69	theme	dodecyl	382:388	arg1	sulfate					390:396	material sodium dodecyl sulfate	366:396	bifunctional material sodium dodecyl sulfate (SDS) coating	353:410	To solve the problem, virus-mimicking nanosized polyelectrolyte complex (PEC) was designed and their capacity in enhancing peroral insulin absorption in combination with bifunctional material sodium dodecyl sulfate (SDS) coating was investigated.
31176647	0	70	theme	Insulin	70:76	arg1	Delivery					78:85	Enhanced Oral Insulin Delivery	56:85	Enhanced Oral Insulin Delivery	56:85	Design of Virus-Mimicking Polyelectrolyte Complexes for Enhanced Oral Insulin Delivery.
31176647	2	71	theme	sodium	375:380	arg1	SDS					399:401	SDS	399:401	SDS	399:401	To solve the problem, virus-mimicking nanosized polyelectrolyte complex (PEC) was designed and their capacity in enhancing peroral insulin absorption in combination with bifunctional material sodium dodecyl sulfate (SDS) coating was investigated.
31176647	2	71	theme	sodium	375:380	arg1	sulfate					390:396	material sodium dodecyl sulfate	366:396	bifunctional material sodium dodecyl sulfate (SDS) coating	353:410	To solve the problem, virus-mimicking nanosized polyelectrolyte complex (PEC) was designed and their capacity in enhancing peroral insulin absorption in combination with bifunctional material sodium dodecyl sulfate (SDS) coating was investigated.
31176647	4	72	theme	relative	956:963	arg1	bioavailability					981:995	2.2-fold higher relative pharmacological bioavailability	940:995	2.2-fold higher relative pharmacological bioavailability than nonmodified CS/insulin PECs	940:1028	Based on the results from both in vitro and in vivo studies, it was concluded that in vitro mucodiffusion and in vivo hypoglycemic effect were dependent on L-Phe graft ratio, with CS-g-N-Phe20.2%/insulin PECs presenting 2.0- to 2.2-fold higher relative pharmacological bioavailability than nonmodified CS/insulin PECs.
30010859	0	0	theme	carbohydrates	84:96	arg1	exudation					71:79	the root exudation	62:79	the root exudation of carbohydrates, which stimulates fungal growth and the biocontrol of the phytopathogen Phytophthora cinnamomi in a tripartite interaction system	62:226	Trichoderma atroviride promotes tomato development and alters the root exudation of carbohydrates, which stimulates fungal growth and the biocontrol of the phytopathogen Phytophthora cinnamomi in a tripartite interaction system.
30010859	5	1	theme	biocontrol	673:682	arg1	capacity					684:691	the biocontrol capacity	669:691	the biocontrol capacity of T. atroviride against the phytopathogen Phytophthora cinnamomi in a tripartite interaction system	669:792	In addition, the biocontrol capacity of T. atroviride against the phytopathogen Phytophthora cinnamomi in a tripartite interaction system was examined.
30010859	1	2	theme	plant	268:272	arg1	growth					274:279	plant growth	268:279	plant growth	268:279	Several species of Trichoderma promote plant growth and help in defense against root pathogens.
30010859	8	3	theme	root	1194:1197	arg1	colonization					1199:1210	root colonization	1194:1210	root colonization by the fungus	1194:1224	Particularly, sucrose was found only during root colonization by the fungus.
30010859	9	4	theme	tripartite	1325:1334	arg1	system					1348:1353	the tripartite interaction system	1321:1353	the tripartite interaction system with P. cinnamomi	1321:1371	Our data show that root-derived sugars enabled a higher Trichoderma growth rate, and that, in the tripartite interaction system with P. cinnamomi, the fungus competes for space and available soil nutrients more efficiently than P. cinnamomi, thereby antagonizing the growth of the phytopathogen.
30010859	0	5	theme	cinnamomi	183:191	arg1	growth					123:128	fungal growth	116:128	fungal growth	116:128	Trichoderma atroviride promotes tomato development and alters the root exudation of carbohydrates, which stimulates fungal growth and the biocontrol of the phytopathogen Phytophthora cinnamomi in a tripartite interaction system.
30010859	0	5	theme	cinnamomi	183:191	arg1	biocontrol					138:147	the biocontrol	134:147	the biocontrol	134:147	Trichoderma atroviride promotes tomato development and alters the root exudation of carbohydrates, which stimulates fungal growth and the biocontrol of the phytopathogen Phytophthora cinnamomi in a tripartite interaction system.
30010859	2	6	theme	chemo-attractive	366:381	arg1	stimuli					383:389	chemo-attractive stimuli	366:389	chemo-attractive stimuli for Trichoderma colonization	366:418	The role of root-exuded carbohydrates as chemo-attractive stimuli for Trichoderma colonization is attracting considerable interest.
30010859	5	7	theme	tripartite	764:773	arg1	system					787:792	a tripartite interaction system	762:792	a tripartite interaction system	762:792	In addition, the biocontrol capacity of T. atroviride against the phytopathogen Phytophthora cinnamomi in a tripartite interaction system was examined.
30010859	9	8	theme	interaction	1336:1346	arg1	system					1348:1353	the tripartite interaction system	1321:1353	the tripartite interaction system with P. cinnamomi	1321:1371	Our data show that root-derived sugars enabled a higher Trichoderma growth rate, and that, in the tripartite interaction system with P. cinnamomi, the fungus competes for space and available soil nutrients more efficiently than P. cinnamomi, thereby antagonizing the growth of the phytopathogen.
30010859	0	9	theme	tripartite	198:207	arg1	system					221:226	a tripartite interaction system	196:226	a tripartite interaction system	196:226	Trichoderma atroviride promotes tomato development and alters the root exudation of carbohydrates, which stimulates fungal growth and the biocontrol of the phytopathogen Phytophthora cinnamomi in a tripartite interaction system.
30010859	6	10	theme	atroviride	851:860	arg1	effects					837:843	the beneficial effects	822:843	the beneficial effects of T. atroviride on plant growth	822:876	We found that the beneficial effects of T. atroviride on plant growth were fine-tuned depending on the progress of interaction.
30010859	5	11	theme	interaction	775:785	arg1	system					787:792	a tripartite interaction system	762:792	a tripartite interaction system	762:792	In addition, the biocontrol capacity of T. atroviride against the phytopathogen Phytophthora cinnamomi in a tripartite interaction system was examined.
30010859	5	12	theme	T.	696:697	arg1	atroviride					699:708	T. atroviride	696:708	T. atroviride	696:708	In addition, the biocontrol capacity of T. atroviride against the phytopathogen Phytophthora cinnamomi in a tripartite interaction system was examined.
30010859	6	13	theme	T.	848:849	arg1	atroviride					851:860	T. atroviride	848:860	T. atroviride	848:860	We found that the beneficial effects of T. atroviride on plant growth were fine-tuned depending on the progress of interaction.
30010859	5	14	from	cinnamomi	749:757	arg1	system					787:792	a tripartite interaction system	762:792	a tripartite interaction system	762:792	In addition, the biocontrol capacity of T. atroviride against the phytopathogen Phytophthora cinnamomi in a tripartite interaction system was examined.
30010859	0	15	theme	fungal	116:121	arg1	growth					123:128	fungal growth	116:128	fungal growth	116:128	Trichoderma atroviride promotes tomato development and alters the root exudation of carbohydrates, which stimulates fungal growth and the biocontrol of the phytopathogen Phytophthora cinnamomi in a tripartite interaction system.
30010859	2	16	theme	considerable	434:445	arg1	interest					447:454	attracting considerable interest	423:454	attracting considerable interest	423:454	The role of root-exuded carbohydrates as chemo-attractive stimuli for Trichoderma colonization is attracting considerable interest.
30010859	2	16	theme	considerable	434:445	arg1	role					329:332	The role	325:332	The role of root-exuded carbohydrates as chemo-attractive stimuli for Trichoderma colonization	325:418	The role of root-exuded carbohydrates as chemo-attractive stimuli for Trichoderma colonization is attracting considerable interest.
30010859	6	17	theme	interaction	923:933	arg1	progress					911:918	the progress	907:918	the progress of interaction	907:933	We found that the beneficial effects of T. atroviride on plant growth were fine-tuned depending on the progress of interaction.
30010859	0	18	theme	interaction	209:219	arg1	system					221:226	a tripartite interaction system	196:226	a tripartite interaction system	196:226	Trichoderma atroviride promotes tomato development and alters the root exudation of carbohydrates, which stimulates fungal growth and the biocontrol of the phytopathogen Phytophthora cinnamomi in a tripartite interaction system.
30010859	7	19	theme	exudate	987:993	arg1	different					1042:1050	different	1042:1050	different	1042:1050	Interestingly, the composition of the carbohydrate exudate from plants interacting with T. atroviride was different from that produced by other treatments and probably provided a nutritional source for the fungus.
30010859	7	19	theme	exudate	987:993	arg1	composition					955:965	the composition	951:965	the composition of the carbohydrate exudate from plants interacting with T. atroviride	951:1036	Interestingly, the composition of the carbohydrate exudate from plants interacting with T. atroviride was different from that produced by other treatments and probably provided a nutritional source for the fungus.
30010859	2	20	theme	attracting	423:432	arg1	interest					447:454	attracting considerable interest	423:454	attracting considerable interest	423:454	The role of root-exuded carbohydrates as chemo-attractive stimuli for Trichoderma colonization is attracting considerable interest.
30010859	2	20	theme	attracting	423:432	arg1	role					329:332	The role	325:332	The role of root-exuded carbohydrates as chemo-attractive stimuli for Trichoderma colonization	325:418	The role of root-exuded carbohydrates as chemo-attractive stimuli for Trichoderma colonization is attracting considerable interest.
30010859	6	21	theme	beneficial	826:835	arg1	effects					837:843	the beneficial effects	822:843	the beneficial effects of T. atroviride on plant growth	822:876	We found that the beneficial effects of T. atroviride on plant growth were fine-tuned depending on the progress of interaction.
30010859	0	22	theme	Trichoderma	0:10	arg1	atroviride					12:21	Trichoderma atroviride	0:21	Trichoderma atroviride	0:21	Trichoderma atroviride promotes tomato development and alters the root exudation of carbohydrates, which stimulates fungal growth and the biocontrol of the phytopathogen Phytophthora cinnamomi in a tripartite interaction system.
30010859	0	23	theme	tomato	32:37	arg1	development					39:49	tomato development	32:49	tomato development	32:49	Trichoderma atroviride promotes tomato development and alters the root exudation of carbohydrates, which stimulates fungal growth and the biocontrol of the phytopathogen Phytophthora cinnamomi in a tripartite interaction system.
30010859	5	24	theme	Phytophthora	736:747	arg1	cinnamomi					749:757	the phytopathogen Phytophthora cinnamomi	718:757	the phytopathogen Phytophthora cinnamomi in a tripartite interaction system	718:792	In addition, the biocontrol capacity of T. atroviride against the phytopathogen Phytophthora cinnamomi in a tripartite interaction system was examined.
30010859	7	25	theme	carbohydrate	974:985	arg1	exudate					987:993	the carbohydrate exudate	970:993	the carbohydrate exudate from plants interacting with T. atroviride	970:1036	Interestingly, the composition of the carbohydrate exudate from plants interacting with T. atroviride was different from that produced by other treatments and probably provided a nutritional source for the fungus.
30010859	7	26	from	composition	955:965	arg1	plants					1000:1005	plants	1000:1005	plants interacting with T. atroviride	1000:1036	Interestingly, the composition of the carbohydrate exudate from plants interacting with T. atroviride was different from that produced by other treatments and probably provided a nutritional source for the fungus.
30010859	9	27	theme	available	1408:1416	arg1	nutrients					1423:1431	available soil nutrients	1408:1431	available soil nutrients	1408:1431	Our data show that root-derived sugars enabled a higher Trichoderma growth rate, and that, in the tripartite interaction system with P. cinnamomi, the fungus competes for space and available soil nutrients more efficiently than P. cinnamomi, thereby antagonizing the growth of the phytopathogen.
30010859	7	28	from	that	1057:1060	arg1	different					1042:1050	different	1042:1050	different	1042:1050	Interestingly, the composition of the carbohydrate exudate from plants interacting with T. atroviride was different from that produced by other treatments and probably provided a nutritional source for the fungus.
30010859	7	28	from	that	1057:1060	arg1	composition					955:965	the composition	951:965	the composition of the carbohydrate exudate from plants interacting with T. atroviride	951:1036	Interestingly, the composition of the carbohydrate exudate from plants interacting with T. atroviride was different from that produced by other treatments and probably provided a nutritional source for the fungus.
30010859	4	29	theme	different	601:609	arg1	stages					611:616	two different stages	597:616	two different stages	597:616	Río Grande) plants in two different stages, before and during root colonization.
30010859	9	30	theme	higher	1276:1281	arg1	rate					1302:1305	a higher Trichoderma growth rate	1274:1305	a higher Trichoderma growth rate	1274:1305	Our data show that root-derived sugars enabled a higher Trichoderma growth rate, and that, in the tripartite interaction system with P. cinnamomi, the fungus competes for space and available soil nutrients more efficiently than P. cinnamomi, thereby antagonizing the growth of the phytopathogen.
30010859	3	31	theme	Trichoderma	509:519	arg1	atroviride					521:530	Trichoderma atroviride	509:530	Trichoderma atroviride	509:530	In this project, we studied the interaction between Trichoderma atroviride and tomato (Lycopersicon esculentum L. cv.
30010859	1	32	theme	root	309:312	arg1	pathogens					314:322	root pathogens	309:322	root pathogens	309:322	Several species of Trichoderma promote plant growth and help in defense against root pathogens.
30010859	9	33	theme	Trichoderma	1283:1293	arg1	rate					1302:1305	a higher Trichoderma growth rate	1274:1305	a higher Trichoderma growth rate	1274:1305	Our data show that root-derived sugars enabled a higher Trichoderma growth rate, and that, in the tripartite interaction system with P. cinnamomi, the fungus competes for space and available soil nutrients more efficiently than P. cinnamomi, thereby antagonizing the growth of the phytopathogen.
30010859	6	34	from	effects	837:843	arg1	growth					871:876	plant growth	865:876	plant growth	865:876	We found that the beneficial effects of T. atroviride on plant growth were fine-tuned depending on the progress of interaction.
30010859	7	35	theme	nutritional	1115:1125	arg1	source					1127:1132	a nutritional source	1113:1132	a nutritional source for the fungus	1113:1147	Interestingly, the composition of the carbohydrate exudate from plants interacting with T. atroviride was different from that produced by other treatments and probably provided a nutritional source for the fungus.
30010859	9	36	theme	growth	1295:1300	arg1	rate					1302:1305	a higher Trichoderma growth rate	1274:1305	a higher Trichoderma growth rate	1274:1305	Our data show that root-derived sugars enabled a higher Trichoderma growth rate, and that, in the tripartite interaction system with P. cinnamomi, the fungus competes for space and available soil nutrients more efficiently than P. cinnamomi, thereby antagonizing the growth of the phytopathogen.
30010859	9	37	link	root-derived	1246:1257	arg1	sugars					1259:1264	root-derived sugars	1246:1264	root-derived sugars	1246:1264	Our data show that root-derived sugars enabled a higher Trichoderma growth rate, and that, in the tripartite interaction system with P. cinnamomi, the fungus competes for space and available soil nutrients more efficiently than P. cinnamomi, thereby antagonizing the growth of the phytopathogen.
30010859	1	38	theme	Several	229:235	arg1	species					237:243	Several species	229:243	Several species of Trichoderma	229:258	Several species of Trichoderma promote plant growth and help in defense against root pathogens.
30010859	5	39	theme	atroviride	699:708	arg1	capacity					684:691	the biocontrol capacity	669:691	the biocontrol capacity of T. atroviride against the phytopathogen Phytophthora cinnamomi in a tripartite interaction system	669:792	In addition, the biocontrol capacity of T. atroviride against the phytopathogen Phytophthora cinnamomi in a tripartite interaction system was examined.
30010859	9	40	theme	phytopathogen	1508:1520	arg1	growth					1494:1499	the growth	1490:1499	the growth of the phytopathogen	1490:1520	Our data show that root-derived sugars enabled a higher Trichoderma growth rate, and that, in the tripartite interaction system with P. cinnamomi, the fungus competes for space and available soil nutrients more efficiently than P. cinnamomi, thereby antagonizing the growth of the phytopathogen.
30010859	7	41	theme	other	1074:1078	arg1	treatments					1080:1089	other treatments	1074:1089	other treatments	1074:1089	Interestingly, the composition of the carbohydrate exudate from plants interacting with T. atroviride was different from that produced by other treatments and probably provided a nutritional source for the fungus.
30010859	3	42	dep	studied	477:483	arg1	L.					568:569	L.	568:569	L.	568:569	In this project, we studied the interaction between Trichoderma atroviride and tomato (Lycopersicon esculentum L. cv.
30010859	2	43	theme	carbohydrates	349:361	arg1	interest					447:454	attracting considerable interest	423:454	attracting considerable interest	423:454	The role of root-exuded carbohydrates as chemo-attractive stimuli for Trichoderma colonization is attracting considerable interest.
30010859	2	43	theme	carbohydrates	349:361	arg1	role					329:332	The role	325:332	The role of root-exuded carbohydrates as chemo-attractive stimuli for Trichoderma colonization	325:418	The role of root-exuded carbohydrates as chemo-attractive stimuli for Trichoderma colonization is attracting considerable interest.
30010859	4	44	theme	Grande	579:584	arg1	plants					587:592	Grande) plants	579:592	Grande) plants	579:592	Río Grande) plants in two different stages, before and during root colonization.
30010859	0	45	theme	Phytophthora	170:181	arg1	cinnamomi					183:191	the phytopathogen Phytophthora cinnamomi	152:191	the phytopathogen Phytophthora cinnamomi	152:191	Trichoderma atroviride promotes tomato development and alters the root exudation of carbohydrates, which stimulates fungal growth and the biocontrol of the phytopathogen Phytophthora cinnamomi in a tripartite interaction system.
30010859	9	46	theme	root-derived	1246:1257	arg1	sugars					1259:1264	root-derived sugars	1246:1264	root-derived sugars	1246:1264	Our data show that root-derived sugars enabled a higher Trichoderma growth rate, and that, in the tripartite interaction system with P. cinnamomi, the fungus competes for space and available soil nutrients more efficiently than P. cinnamomi, thereby antagonizing the growth of the phytopathogen.
30010859	7	47	theme	T.	1024:1025	arg1	atroviride					1027:1036	T. atroviride	1024:1036	T. atroviride	1024:1036	Interestingly, the composition of the carbohydrate exudate from plants interacting with T. atroviride was different from that produced by other treatments and probably provided a nutritional source for the fungus.
30010859	2	48	theme	root-exuded	337:347	arg1	carbohydrates					349:361	root-exuded carbohydrates	337:361	root-exuded carbohydrates	337:361	The role of root-exuded carbohydrates as chemo-attractive stimuli for Trichoderma colonization is attracting considerable interest.
30010859	9	49	theme	soil	1418:1421	arg1	nutrients					1423:1431	available soil nutrients	1408:1431	available soil nutrients	1408:1431	Our data show that root-derived sugars enabled a higher Trichoderma growth rate, and that, in the tripartite interaction system with P. cinnamomi, the fungus competes for space and available soil nutrients more efficiently than P. cinnamomi, thereby antagonizing the growth of the phytopathogen.
30010859	4	50	theme	root	637:640	arg1	colonization					642:653	root colonization	637:653	root colonization	637:653	Río Grande) plants in two different stages, before and during root colonization.
30010859	9	51	with	system	1348:1353	arg1	cinnamomi					1363:1371	cinnamomi	1363:1371	cinnamomi	1363:1371	Our data show that root-derived sugars enabled a higher Trichoderma growth rate, and that, in the tripartite interaction system with P. cinnamomi, the fungus competes for space and available soil nutrients more efficiently than P. cinnamomi, thereby antagonizing the growth of the phytopathogen.
30010859	6	52	theme	plant	865:869	arg1	growth					871:876	plant growth	865:876	plant growth	865:876	We found that the beneficial effects of T. atroviride on plant growth were fine-tuned depending on the progress of interaction.
30010859	2	53	theme	Trichoderma	395:405	arg1	colonization					407:418	Trichoderma colonization	395:418	Trichoderma colonization	395:418	The role of root-exuded carbohydrates as chemo-attractive stimuli for Trichoderma colonization is attracting considerable interest.
30010859	1	54	theme	Trichoderma	248:258	arg1	species					237:243	Several species	229:243	Several species of Trichoderma	229:258	Several species of Trichoderma promote plant growth and help in defense against root pathogens.
30010859	0	55	theme	root	66:69	arg1	exudation					71:79	the root exudation	62:79	the root exudation of carbohydrates, which stimulates fungal growth and the biocontrol of the phytopathogen Phytophthora cinnamomi in a tripartite interaction system	62:226	Trichoderma atroviride promotes tomato development and alters the root exudation of carbohydrates, which stimulates fungal growth and the biocontrol of the phytopathogen Phytophthora cinnamomi in a tripartite interaction system.
30010859	7	56	from	plants	1000:1005	arg1	exudate					987:993	the carbohydrate exudate	970:993	the carbohydrate exudate from plants interacting with T. atroviride	970:1036	Interestingly, the composition of the carbohydrate exudate from plants interacting with T. atroviride was different from that produced by other treatments and probably provided a nutritional source for the fungus.
30010859	7	56	from	plants	1000:1005	arg1	different					1042:1050	different	1042:1050	different	1042:1050	Interestingly, the composition of the carbohydrate exudate from plants interacting with T. atroviride was different from that produced by other treatments and probably provided a nutritional source for the fungus.
30010859	7	56	from	plants	1000:1005	arg1	composition					955:965	the composition	951:965	the composition of the carbohydrate exudate from plants interacting with T. atroviride	951:1036	Interestingly, the composition of the carbohydrate exudate from plants interacting with T. atroviride was different from that produced by other treatments and probably provided a nutritional source for the fungus.
29723621	8	0	theme	Coated	1160:1165	arg1	cheese					1174:1179	Coated Karish cheese	1160:1179	Coated Karish cheese	1160:1179	Coated Karish cheese retained acceptable quality until the end of the storage period, while uncoated developed surface fungi growth and deteriorated quality after 15 days.
29723621	4	1	theme	morphological	695:707	arg1	features					709:716	homogeneous, compact morphological features	674:716	homogeneous, compact morphological features	674:716	The developed CS/PVA/Gy/TiO2-NPs bionanocomposites exhibited homogeneous, compact morphological features and enhanced mechanical and barrier properties.
29723621	9	2	theme	highest	1413:1419	arg1	acceptability					1421:1433	the highest acceptability	1409:1433	the highest acceptability	1409:1433	Karish cheese coated with the bionanocomposite containing 3% TiO2-NPs ranked the highest acceptability at the end of storage period.
29723621	1	3	theme	consideration	153:165	arg1	alternatives					170:181	alternatives	170:181	alternatives for a broad group of commercial materials based on petroleum-derived compounds and used for coating cheese	170:288	Bionanocomposites have attracted a tremendous level of consideration as alternatives for a broad group of commercial materials based on petroleum-derived compounds and used for coating cheese.
29723621	1	3	theme	consideration	153:165	arg1	level					144:148	a tremendous level	131:148	a tremendous level of consideration	131:165	Bionanocomposites have attracted a tremendous level of consideration as alternatives for a broad group of commercial materials based on petroleum-derived compounds and used for coating cheese.
29723621	7	4	from	quality	1065:1071	arg1	losses					1021:1026	the weight losses	1010:1026	the weight losses	1010:1026	Changes in the weight losses, cheese composition, microbiological quality, textural parameters, and sensory properties were followed during storage for 25 days.
29723621	5	5	theme	prepared	776:783	arg1	bionanocomposite					785:800	the prepared bionanocomposite	772:800	the prepared bionanocomposite	772:800	Also, the prepared bionanocomposite exhibited variable inhibitory effects against several pathogenic bacteria and fungi.
29723621	8	6	theme	storage	1230:1236	arg1	period					1238:1243	the storage period	1226:1243	the storage period	1226:1243	Coated Karish cheese retained acceptable quality until the end of the storage period, while uncoated developed surface fungi growth and deteriorated quality after 15 days.
29723621	4	7	theme	barrier	746:752	arg1	properties					754:763	mechanical and barrier properties	731:763	properties	754:763	The developed CS/PVA/Gy/TiO2-NPs bionanocomposites exhibited homogeneous, compact morphological features and enhanced mechanical and barrier properties.
29723621	6	8	theme	prepared	923:930	arg1	bionanocomposite					932:947	the prepared bionanocomposite	919:947	the prepared bionanocomposite containing 1, 2 and 3% TiO2-NPs	919:979	Karish was made and coated with the prepared bionanocomposite containing 1, 2 and 3% TiO2-NPs, and cold stored.
29723621	2	9	theme	Sustainable	291:301	arg1	materials					346:354	Sustainable, economical and environmentally compatible materials	291:354	Sustainable, economical and environmentally compatible materials based on chitosan (CS), poly vinyl alcohol (PVA), glycerol (Gy) and titanium dioxide nanoparticles (TiO2-NPs)	291:464	Sustainable, economical and environmentally compatible materials based on chitosan (CS), poly vinyl alcohol (PVA), glycerol (Gy) and titanium dioxide nanoparticles (TiO2-NPs) were prepared.
29723621	1	10	theme	coating	275:281	arg1	cheese					283:288	coating cheese	275:288	coating cheese	275:288	Bionanocomposites have attracted a tremendous level of consideration as alternatives for a broad group of commercial materials based on petroleum-derived compounds and used for coating cheese.
29723621	8	11	theme	acceptable	1190:1199	arg1	quality					1201:1207	acceptable quality	1190:1207	acceptable quality	1190:1207	Coated Karish cheese retained acceptable quality until the end of the storage period, while uncoated developed surface fungi growth and deteriorated quality after 15 days.
29723621	9	12	theme	Karish	1332:1337	arg1	cheese					1339:1344	Karish cheese	1332:1344	Karish cheese coated with the bionanocomposite containing 3% TiO2-NPs	1332:1400	Karish cheese coated with the bionanocomposite containing 3% TiO2-NPs ranked the highest acceptability at the end of storage period.
29723621	7	13	theme	sensory	1099:1105	arg1	properties					1107:1116	sensory properties	1099:1116	sensory properties	1099:1116	Changes in the weight losses, cheese composition, microbiological quality, textural parameters, and sensory properties were followed during storage for 25 days.
29723621	8	14	theme	deteriorated	1296:1307	arg1	quality					1309:1315	deteriorated quality	1296:1315	deteriorated quality	1296:1315	Coated Karish cheese retained acceptable quality until the end of the storage period, while uncoated developed surface fungi growth and deteriorated quality after 15 days.
29723621	3	15	theme	prepared	495:502	arg1	CS/PVA/Gy/TiO2-NPs					523:540	CS/PVA/Gy/TiO2-NPs	523:540	CS/PVA/Gy/TiO2-NPs	523:540	Moreover, the prepared bionanocomposites (CS/PVA/Gy/TiO2-NPs) were characterized using XRD, SEM, TEM, WVTR and mechanical strength.
29723621	3	15	theme	prepared	495:502	arg1	bionanocomposites					504:520	the prepared bionanocomposites	491:520	the prepared bionanocomposites (CS/PVA/Gy/TiO2-NPs)	491:541	Moreover, the prepared bionanocomposites (CS/PVA/Gy/TiO2-NPs) were characterized using XRD, SEM, TEM, WVTR and mechanical strength.
29723621	7	16	from	Changes	999:1005	arg1	losses					1021:1026	the weight losses	1010:1026	the weight losses	1010:1026	Changes in the weight losses, cheese composition, microbiological quality, textural parameters, and sensory properties were followed during storage for 25 days.
29723621	4	17	theme	CS/PVA/Gy/TiO2-NPs	627:644	arg1	bionanocomposites					646:662	The developed CS/PVA/Gy/TiO2-NPs bionanocomposites	613:662	The developed CS/PVA/Gy/TiO2-NPs bionanocomposites	613:662	The developed CS/PVA/Gy/TiO2-NPs bionanocomposites exhibited homogeneous, compact morphological features and enhanced mechanical and barrier properties.
29723621	5	18	theme	inhibitory	821:830	arg1	effects					832:838	variable inhibitory effects	812:838	variable inhibitory effects	812:838	Also, the prepared bionanocomposite exhibited variable inhibitory effects against several pathogenic bacteria and fungi.
29723621	1	19	link	petroleum-derived	234:250	arg1	compounds					252:260	petroleum-derived compounds	234:260	petroleum-derived compounds	234:260	Bionanocomposites have attracted a tremendous level of consideration as alternatives for a broad group of commercial materials based on petroleum-derived compounds and used for coating cheese.
29723621	4	20	dep	homogeneous	674:684	arg1	compact					687:693	compact	687:693	compact	687:693	The developed CS/PVA/Gy/TiO2-NPs bionanocomposites exhibited homogeneous, compact morphological features and enhanced mechanical and barrier properties.
29723621	7	21	from	parameters	1083:1092	arg1	losses					1021:1026	the weight losses	1010:1026	the weight losses	1010:1026	Changes in the weight losses, cheese composition, microbiological quality, textural parameters, and sensory properties were followed during storage for 25 days.
29723621	2	22	theme	alcohol	391:397	arg1	TiO2-NPs					456:463	TiO2-NPs	456:463	TiO2-NPs	456:463	Sustainable, economical and environmentally compatible materials based on chitosan (CS), poly vinyl alcohol (PVA), glycerol (Gy) and titanium dioxide nanoparticles (TiO2-NPs) were prepared.
29723621	2	22	theme	alcohol	391:397	arg1	nanoparticles					441:453	chitosan (CS), poly vinyl alcohol (PVA), glycerol (Gy) and titanium dioxide nanoparticles	365:453	chitosan (CS), poly vinyl alcohol (PVA), glycerol (Gy) and titanium dioxide nanoparticles (TiO2-NPs)	365:464	Sustainable, economical and environmentally compatible materials based on chitosan (CS), poly vinyl alcohol (PVA), glycerol (Gy) and titanium dioxide nanoparticles (TiO2-NPs) were prepared.
29723621	0	23	theme	bionanocomposite	6:21	arg1	materials					23:31	Novel bionanocomposite materials	0:31	Novel bionanocomposite materials used for packaging	0:50	Novel bionanocomposite materials used for packaging skimmed milk acid coagulated cheese (Karish).
29723621	2	24	theme	compatible	335:344	arg1	materials					346:354	Sustainable, economical and environmentally compatible materials	291:354	Sustainable, economical and environmentally compatible materials based on chitosan (CS), poly vinyl alcohol (PVA), glycerol (Gy) and titanium dioxide nanoparticles (TiO2-NPs)	291:464	Sustainable, economical and environmentally compatible materials based on chitosan (CS), poly vinyl alcohol (PVA), glycerol (Gy) and titanium dioxide nanoparticles (TiO2-NPs) were prepared.
29723621	2	25	theme	vinyl	385:389	arg1	alcohol					391:397	poly vinyl alcohol	380:397	poly vinyl alcohol (PVA)	380:403	Sustainable, economical and environmentally compatible materials based on chitosan (CS), poly vinyl alcohol (PVA), glycerol (Gy) and titanium dioxide nanoparticles (TiO2-NPs) were prepared.
29723621	2	25	theme	vinyl	385:389	arg1	PVA					400:402	PVA	400:402	PVA	400:402	Sustainable, economical and environmentally compatible materials based on chitosan (CS), poly vinyl alcohol (PVA), glycerol (Gy) and titanium dioxide nanoparticles (TiO2-NPs) were prepared.
29723621	0	26	theme	Novel	0:4	arg1	materials					23:31	Novel bionanocomposite materials	0:31	Novel bionanocomposite materials used for packaging	0:50	Novel bionanocomposite materials used for packaging skimmed milk acid coagulated cheese (Karish).
29723621	2	27	theme	poly	380:383	arg1	alcohol					391:397	poly vinyl alcohol	380:397	poly vinyl alcohol (PVA)	380:403	Sustainable, economical and environmentally compatible materials based on chitosan (CS), poly vinyl alcohol (PVA), glycerol (Gy) and titanium dioxide nanoparticles (TiO2-NPs) were prepared.
29723621	2	27	theme	poly	380:383	arg1	PVA					400:402	PVA	400:402	PVA	400:402	Sustainable, economical and environmentally compatible materials based on chitosan (CS), poly vinyl alcohol (PVA), glycerol (Gy) and titanium dioxide nanoparticles (TiO2-NPs) were prepared.
29723621	5	28	theme	several	848:854	arg1	bacteria					867:874	several pathogenic bacteria	848:874	several pathogenic bacteria	848:874	Also, the prepared bionanocomposite exhibited variable inhibitory effects against several pathogenic bacteria and fungi.
29723621	7	29	theme	textural	1074:1081	arg1	parameters					1083:1092	textural parameters	1074:1092	textural parameters	1074:1092	Changes in the weight losses, cheese composition, microbiological quality, textural parameters, and sensory properties were followed during storage for 25 days.
29723621	9	30	theme	period	1457:1462	arg1	end					1442:1444	the end	1438:1444	the end of storage period	1438:1462	Karish cheese coated with the bionanocomposite containing 3% TiO2-NPs ranked the highest acceptability at the end of storage period.
29723621	5	31	theme	variable	812:819	arg1	effects					832:838	variable inhibitory effects	812:838	variable inhibitory effects	812:838	Also, the prepared bionanocomposite exhibited variable inhibitory effects against several pathogenic bacteria and fungi.
29723621	2	32	theme	glycerol	406:413	arg1	TiO2-NPs					456:463	TiO2-NPs	456:463	TiO2-NPs	456:463	Sustainable, economical and environmentally compatible materials based on chitosan (CS), poly vinyl alcohol (PVA), glycerol (Gy) and titanium dioxide nanoparticles (TiO2-NPs) were prepared.
29723621	2	32	theme	glycerol	406:413	arg1	nanoparticles					441:453	chitosan (CS), poly vinyl alcohol (PVA), glycerol (Gy) and titanium dioxide nanoparticles	365:453	chitosan (CS), poly vinyl alcohol (PVA), glycerol (Gy) and titanium dioxide nanoparticles (TiO2-NPs)	365:464	Sustainable, economical and environmentally compatible materials based on chitosan (CS), poly vinyl alcohol (PVA), glycerol (Gy) and titanium dioxide nanoparticles (TiO2-NPs) were prepared.
29723621	5	33	theme	pathogenic	856:865	arg1	bacteria					867:874	several pathogenic bacteria	848:874	several pathogenic bacteria	848:874	Also, the prepared bionanocomposite exhibited variable inhibitory effects against several pathogenic bacteria and fungi.
29723621	2	34	theme	economical	304:313	arg1	materials					346:354	Sustainable, economical and environmentally compatible materials	291:354	Sustainable, economical and environmentally compatible materials based on chitosan (CS), poly vinyl alcohol (PVA), glycerol (Gy) and titanium dioxide nanoparticles (TiO2-NPs)	291:464	Sustainable, economical and environmentally compatible materials based on chitosan (CS), poly vinyl alcohol (PVA), glycerol (Gy) and titanium dioxide nanoparticles (TiO2-NPs) were prepared.
29723621	9	35	theme	storage	1449:1455	arg1	period					1457:1462	storage period	1449:1462	storage period	1449:1462	Karish cheese coated with the bionanocomposite containing 3% TiO2-NPs ranked the highest acceptability at the end of storage period.
29723621	8	36	theme	surface	1271:1277	arg1	growth					1285:1290	surface fungi growth	1271:1290	surface fungi growth	1271:1290	Coated Karish cheese retained acceptable quality until the end of the storage period, while uncoated developed surface fungi growth and deteriorated quality after 15 days.
29723621	7	37	from	properties	1107:1116	arg1	losses					1021:1026	the weight losses	1010:1026	the weight losses	1010:1026	Changes in the weight losses, cheese composition, microbiological quality, textural parameters, and sensory properties were followed during storage for 25 days.
29723621	1	38	theme	broad	189:193	arg1	group					195:199	a broad group	187:199	a broad group of commercial materials based on petroleum-derived compounds and used for coating cheese	187:288	Bionanocomposites have attracted a tremendous level of consideration as alternatives for a broad group of commercial materials based on petroleum-derived compounds and used for coating cheese.
29723621	1	38	theme	broad	189:193	arg1	materials					215:223	commercial materials	204:223	commercial materials based on petroleum-derived compounds and used for coating cheese	204:288	Bionanocomposites have attracted a tremendous level of consideration as alternatives for a broad group of commercial materials based on petroleum-derived compounds and used for coating cheese.
29723621	1	39	theme	commercial	204:213	arg1	materials					215:223	commercial materials	204:223	commercial materials based on petroleum-derived compounds and used for coating cheese	204:288	Bionanocomposites have attracted a tremendous level of consideration as alternatives for a broad group of commercial materials based on petroleum-derived compounds and used for coating cheese.
29723621	6	40	contain	containing	949:958	arg2	TiO2-NPs					972:979	1, 2 and 3% TiO2-NPs	960:979	1, 2 and 3% TiO2-NPs	960:979	Karish was made and coated with the prepared bionanocomposite containing 1, 2 and 3% TiO2-NPs, and cold stored.
29723621	6	40	contain	containing	949:958	arg1	bionanocomposite					932:947	the prepared bionanocomposite	919:947	the prepared bionanocomposite containing 1, 2 and 3% TiO2-NPs	919:979	Karish was made and coated with the prepared bionanocomposite containing 1, 2 and 3% TiO2-NPs, and cold stored.
29723621	9	41	theme	3	1390:1390	arg1	%					1391:1391	%	1391:1391	%	1391:1391	Karish cheese coated with the bionanocomposite containing 3% TiO2-NPs ranked the highest acceptability at the end of storage period.
29723621	3	42	theme	mechanical	592:601	arg1	XRD					568:570	XRD	568:570	XRD	568:570	Moreover, the prepared bionanocomposites (CS/PVA/Gy/TiO2-NPs) were characterized using XRD, SEM, TEM, WVTR and mechanical strength.
29723621	3	42	theme	mechanical	592:601	arg1	strength					603:610	mechanical strength	592:610	mechanical strength	592:610	Moreover, the prepared bionanocomposites (CS/PVA/Gy/TiO2-NPs) were characterized using XRD, SEM, TEM, WVTR and mechanical strength.
29723621	9	43	contain	containing	1379:1388	arg1	bionanocomposite					1362:1377	the bionanocomposite	1358:1377	the bionanocomposite containing 3% TiO2-NPs	1358:1400	Karish cheese coated with the bionanocomposite containing 3% TiO2-NPs ranked the highest acceptability at the end of storage period.
29723621	9	43	contain	containing	1379:1388	arg2	TiO2-NPs					1393:1400	3% TiO2-NPs	1390:1400	3% TiO2-NPs	1390:1400	Karish cheese coated with the bionanocomposite containing 3% TiO2-NPs ranked the highest acceptability at the end of storage period.
29723621	1	44	theme	materials	215:223	arg1	group					195:199	a broad group	187:199	a broad group of commercial materials based on petroleum-derived compounds and used for coating cheese	187:288	Bionanocomposites have attracted a tremendous level of consideration as alternatives for a broad group of commercial materials based on petroleum-derived compounds and used for coating cheese.
29723621	1	44	theme	materials	215:223	arg1	materials					215:223	commercial materials	204:223	commercial materials based on petroleum-derived compounds and used for coating cheese	204:288	Bionanocomposites have attracted a tremendous level of consideration as alternatives for a broad group of commercial materials based on petroleum-derived compounds and used for coating cheese.
29723621	2	45	theme	dioxide	433:439	arg1	TiO2-NPs					456:463	TiO2-NPs	456:463	TiO2-NPs	456:463	Sustainable, economical and environmentally compatible materials based on chitosan (CS), poly vinyl alcohol (PVA), glycerol (Gy) and titanium dioxide nanoparticles (TiO2-NPs) were prepared.
29723621	2	45	theme	dioxide	433:439	arg1	nanoparticles					441:453	chitosan (CS), poly vinyl alcohol (PVA), glycerol (Gy) and titanium dioxide nanoparticles	365:453	chitosan (CS), poly vinyl alcohol (PVA), glycerol (Gy) and titanium dioxide nanoparticles (TiO2-NPs)	365:464	Sustainable, economical and environmentally compatible materials based on chitosan (CS), poly vinyl alcohol (PVA), glycerol (Gy) and titanium dioxide nanoparticles (TiO2-NPs) were prepared.
29723621	9	46	theme	%	1391:1391	arg1	TiO2-NPs					1393:1400	3% TiO2-NPs	1390:1400	3% TiO2-NPs	1390:1400	Karish cheese coated with the bionanocomposite containing 3% TiO2-NPs ranked the highest acceptability at the end of storage period.
29723621	0	47	theme	acid	65:68	arg1	cheese					81:86	milk acid coagulated cheese	60:86	milk acid coagulated cheese (Karish)	60:95	Novel bionanocomposite materials used for packaging skimmed milk acid coagulated cheese (Karish).
29723621	0	47	theme	acid	65:68	arg1	Karish					89:94	Karish	89:94	Karish	89:94	Novel bionanocomposite materials used for packaging skimmed milk acid coagulated cheese (Karish).
29723621	2	48	theme	chitosan	365:372	arg1	TiO2-NPs					456:463	TiO2-NPs	456:463	TiO2-NPs	456:463	Sustainable, economical and environmentally compatible materials based on chitosan (CS), poly vinyl alcohol (PVA), glycerol (Gy) and titanium dioxide nanoparticles (TiO2-NPs) were prepared.
29723621	2	48	theme	chitosan	365:372	arg1	nanoparticles					441:453	chitosan (CS), poly vinyl alcohol (PVA), glycerol (Gy) and titanium dioxide nanoparticles	365:453	chitosan (CS), poly vinyl alcohol (PVA), glycerol (Gy) and titanium dioxide nanoparticles (TiO2-NPs)	365:464	Sustainable, economical and environmentally compatible materials based on chitosan (CS), poly vinyl alcohol (PVA), glycerol (Gy) and titanium dioxide nanoparticles (TiO2-NPs) were prepared.
29723621	2	49	theme	titanium	424:431	arg1	dioxide					433:439	titanium dioxide	424:439	titanium dioxide	424:439	Sustainable, economical and environmentally compatible materials based on chitosan (CS), poly vinyl alcohol (PVA), glycerol (Gy) and titanium dioxide nanoparticles (TiO2-NPs) were prepared.
29723621	7	50	theme	microbiological	1049:1063	arg1	quality					1065:1071	microbiological quality	1049:1071	microbiological quality	1049:1071	Changes in the weight losses, cheese composition, microbiological quality, textural parameters, and sensory properties were followed during storage for 25 days.
29723621	6	51	dep	%	970:970	arg1	3					969:969	3	969:969	3	969:969	Karish was made and coated with the prepared bionanocomposite containing 1, 2 and 3% TiO2-NPs, and cold stored.
29723621	6	51	dep	%	970:970	arg1	2					963:963	2	963:963	2	963:963	Karish was made and coated with the prepared bionanocomposite containing 1, 2 and 3% TiO2-NPs, and cold stored.
29723621	7	52	theme	cheese	1029:1034	arg1	composition					1036:1046	cheese composition	1029:1046	cheese composition	1029:1046	Changes in the weight losses, cheese composition, microbiological quality, textural parameters, and sensory properties were followed during storage for 25 days.
29723621	6	53	dep	TiO2-NPs	972:979	arg1	%					970:970	%	970:970	%	970:970	Karish was made and coated with the prepared bionanocomposite containing 1, 2 and 3% TiO2-NPs, and cold stored.
29723621	7	54	theme	weight	1014:1019	arg1	losses					1021:1026	the weight losses	1010:1026	the weight losses	1010:1026	Changes in the weight losses, cheese composition, microbiological quality, textural parameters, and sensory properties were followed during storage for 25 days.
29723621	4	55	theme	mechanical	731:740	arg1	properties					754:763	mechanical and barrier properties	731:763	properties	754:763	The developed CS/PVA/Gy/TiO2-NPs bionanocomposites exhibited homogeneous, compact morphological features and enhanced mechanical and barrier properties.
29723621	1	56	dep	materials	215:223	arg1	used					266:269	used	266:269	used for coating cheese	266:288	Bionanocomposites have attracted a tremendous level of consideration as alternatives for a broad group of commercial materials based on petroleum-derived compounds and used for coating cheese.
29723621	1	56	dep	materials	215:223	arg1	based					225:229	based	225:229	based on petroleum-derived compounds	225:260	Bionanocomposites have attracted a tremendous level of consideration as alternatives for a broad group of commercial materials based on petroleum-derived compounds and used for coating cheese.
29723621	1	57	theme	tremendous	133:142	arg1	alternatives					170:181	alternatives	170:181	alternatives for a broad group of commercial materials based on petroleum-derived compounds and used for coating cheese	170:288	Bionanocomposites have attracted a tremendous level of consideration as alternatives for a broad group of commercial materials based on petroleum-derived compounds and used for coating cheese.
29723621	1	57	theme	tremendous	133:142	arg1	level					144:148	a tremendous level	131:148	a tremendous level of consideration	131:165	Bionanocomposites have attracted a tremendous level of consideration as alternatives for a broad group of commercial materials based on petroleum-derived compounds and used for coating cheese.
29723621	8	58	theme	period	1238:1243	arg1	end					1219:1221	the end	1215:1221	the end of the storage period	1215:1243	Coated Karish cheese retained acceptable quality until the end of the storage period, while uncoated developed surface fungi growth and deteriorated quality after 15 days.
29723621	7	59	from	composition	1036:1046	arg1	losses					1021:1026	the weight losses	1010:1026	the weight losses	1010:1026	Changes in the weight losses, cheese composition, microbiological quality, textural parameters, and sensory properties were followed during storage for 25 days.
29723621	0	60	theme	coagulated	70:79	arg1	cheese					81:86	milk acid coagulated cheese	60:86	milk acid coagulated cheese (Karish)	60:95	Novel bionanocomposite materials used for packaging skimmed milk acid coagulated cheese (Karish).
29723621	0	60	theme	coagulated	70:79	arg1	Karish					89:94	Karish	89:94	Karish	89:94	Novel bionanocomposite materials used for packaging skimmed milk acid coagulated cheese (Karish).
29723621	8	61	theme	fungi	1279:1283	arg1	growth					1285:1290	surface fungi growth	1271:1290	surface fungi growth	1271:1290	Coated Karish cheese retained acceptable quality until the end of the storage period, while uncoated developed surface fungi growth and deteriorated quality after 15 days.
29723621	4	62	theme	developed	617:625	arg1	bionanocomposites					646:662	The developed CS/PVA/Gy/TiO2-NPs bionanocomposites	613:662	The developed CS/PVA/Gy/TiO2-NPs bionanocomposites	613:662	The developed CS/PVA/Gy/TiO2-NPs bionanocomposites exhibited homogeneous, compact morphological features and enhanced mechanical and barrier properties.
29723621	1	63	theme	petroleum-derived	234:250	arg1	compounds					252:260	petroleum-derived compounds	234:260	petroleum-derived compounds	234:260	Bionanocomposites have attracted a tremendous level of consideration as alternatives for a broad group of commercial materials based on petroleum-derived compounds and used for coating cheese.
29723621	0	64	theme	milk	60:63	arg1	cheese					81:86	milk acid coagulated cheese	60:86	milk acid coagulated cheese (Karish)	60:95	Novel bionanocomposite materials used for packaging skimmed milk acid coagulated cheese (Karish).
29723621	0	64	theme	milk	60:63	arg1	Karish					89:94	Karish	89:94	Karish	89:94	Novel bionanocomposite materials used for packaging skimmed milk acid coagulated cheese (Karish).
29723621	4	65	theme	homogeneous	674:684	arg1	features					709:716	homogeneous, compact morphological features	674:716	homogeneous, compact morphological features	674:716	The developed CS/PVA/Gy/TiO2-NPs bionanocomposites exhibited homogeneous, compact morphological features and enhanced mechanical and barrier properties.
29723621	8	66	theme	Karish	1167:1172	arg1	cheese					1174:1179	Coated Karish cheese	1160:1179	Coated Karish cheese	1160:1179	Coated Karish cheese retained acceptable quality until the end of the storage period, while uncoated developed surface fungi growth and deteriorated quality after 15 days.
29641895	4	0	theme	HA	931:932	arg1	density					942:948	higher HA coating density	924:948	higher HA coating density on NCs, termed as NPs-3,	924:973	The targeting mechanism was studied on the established injured endothelium-macrophage coculture system, which revealed that modification of higher molecular weight HA and higher HA coating density on NCs, termed as NPs-3, improved the intracellular uptake of nanoparticles by macrophages.
29641895	9	1	theme	efficient	1780:1788	arg1	delivery					1815:1822	efficient macrophage-targeted gene delivery	1780:1822	efficient macrophage-targeted gene delivery	1780:1822	Collectively, the HA-coated CPP NCs were promising nanocarriers for efficient macrophage-targeted gene delivery and antiatherogenic therapy.
29641895	4	2	theme	density	942:948	arg1	modification					877:888	modification	877:888	modification of higher molecular weight HA and higher HA coating density on NCs, termed as NPs-3,	877:973	The targeting mechanism was studied on the established injured endothelium-macrophage coculture system, which revealed that modification of higher molecular weight HA and higher HA coating density on NCs, termed as NPs-3, improved the intracellular uptake of nanoparticles by macrophages.
29641895	1	3	theme	targeted	304:311	arg1	delivery					318:325	targeted gene delivery	304:325	targeted gene delivery to macrophages	304:340	Hyaluronic-acid (HA)-coated LOX-1-specific siRNA-condensed cell-penetrating peptide (CPP) nanocomplexes (NCs) were developed for targeted gene delivery to macrophages and suppression of lipid accumulation.
29641895	4	4	theme	weight	910:915	arg1	HA					917:918	higher molecular weight HA	893:918	higher molecular weight HA	893:918	The targeting mechanism was studied on the established injured endothelium-macrophage coculture system, which revealed that modification of higher molecular weight HA and higher HA coating density on NCs, termed as NPs-3, improved the intracellular uptake of nanoparticles by macrophages.
29641895	7	5	from	imaging	1373:1379	arg1	results					1352:1358	results	1352:1358	results from ex vivo imaging	1352:1379	Compared with NCs, HA decoration showed enhanced atherosclerotic-lesion-targeting efficiency, proven by results from ex vivo imaging.
29641895	1	6	theme	LOX-1-specific	203:216	arg1	CPP					260:262	CPP	260:262	CPP	260:262	Hyaluronic-acid (HA)-coated LOX-1-specific siRNA-condensed cell-penetrating peptide (CPP) nanocomplexes (NCs) were developed for targeted gene delivery to macrophages and suppression of lipid accumulation.
29641895	1	6	theme	LOX-1-specific	203:216	arg1	peptide					251:257	LOX-1-specific siRNA-condensed cell-penetrating peptide	203:257	Hyaluronic-acid (HA)-coated LOX-1-specific siRNA-condensed cell-penetrating peptide (CPP) nanocomplexes (NCs)	175:283	Hyaluronic-acid (HA)-coated LOX-1-specific siRNA-condensed cell-penetrating peptide (CPP) nanocomplexes (NCs) were developed for targeted gene delivery to macrophages and suppression of lipid accumulation.
29641895	0	7	from	Macrophages	131:141	arg1	Therapy					166:172	Antiatherosclerotic Therapy	146:172	Antiatherosclerotic Therapy	146:172	Fine Tuning of Core-Shell Structure of Hyaluronic Acid/Cell-Penetrating Peptides/siRNA Nanoparticles for Enhanced Gene Delivery to Macrophages in Antiatherosclerotic Therapy.
29641895	1	8	theme	cell-penetrating	234:249	arg1	CPP					260:262	CPP	260:262	CPP	260:262	Hyaluronic-acid (HA)-coated LOX-1-specific siRNA-condensed cell-penetrating peptide (CPP) nanocomplexes (NCs) were developed for targeted gene delivery to macrophages and suppression of lipid accumulation.
29641895	1	8	theme	cell-penetrating	234:249	arg1	peptide					251:257	LOX-1-specific siRNA-condensed cell-penetrating peptide	203:257	Hyaluronic-acid (HA)-coated LOX-1-specific siRNA-condensed cell-penetrating peptide (CPP) nanocomplexes (NCs)	175:283	Hyaluronic-acid (HA)-coated LOX-1-specific siRNA-condensed cell-penetrating peptide (CPP) nanocomplexes (NCs) were developed for targeted gene delivery to macrophages and suppression of lipid accumulation.
29641895	2	9	theme	ultimate	605:612	arg1	location					614:621	the ultimate location	601:621	the ultimate location into macrophages	601:638	The HA coating facilitated the accumulation of nanoparticles at leaky endothelium overexpressing CD44 receptors and was further degraded by hyaluronidase (HAase) intraplaques for exposing the naked CPP NCs and achieving the ultimate location into macrophages.
29641895	8	10	theme	lipid	1569:1573	arg1	accumulation					1575:1586	lipid accumulation	1569:1586	lipid accumulation	1569:1586	Furthermore, atheroprotective efficacy study in apoE-deficient mice showed that NPs-3 had the best potent efficacy, which was demonstrated by the fewest atherosclerotic lesions sizes and lipid accumulation, the lowest macrophage infiltration, and the lowest expression of monocyte chemoattractant protein-1 (MCP-1), respectively.
29641895	8	11	theme	atheroprotective	1395:1410	arg1	study					1421:1425	atheroprotective efficacy study	1395:1425	atheroprotective efficacy study in apoE-deficient mice	1395:1448	Furthermore, atheroprotective efficacy study in apoE-deficient mice showed that NPs-3 had the best potent efficacy, which was demonstrated by the fewest atherosclerotic lesions sizes and lipid accumulation, the lowest macrophage infiltration, and the lowest expression of monocyte chemoattractant protein-1 (MCP-1), respectively.
29641895	3	12	theme	surface	645:651	arg1	coating					653:659	The surface coating	641:659	The surface coating of HA	641:665	The surface coating of HA was verified by the increased particle size, inverted zeta potential, and TEM images.
29641895	4	13	from	modification	877:888	arg1	NCs					953:955	NCs	953:955	NCs	953:955	The targeting mechanism was studied on the established injured endothelium-macrophage coculture system, which revealed that modification of higher molecular weight HA and higher HA coating density on NCs, termed as NPs-3, improved the intracellular uptake of nanoparticles by macrophages.
29641895	9	14	theme	gene	1810:1813	arg1	delivery					1815:1822	efficient macrophage-targeted gene delivery	1780:1822	efficient macrophage-targeted gene delivery	1780:1822	Collectively, the HA-coated CPP NCs were promising nanocarriers for efficient macrophage-targeted gene delivery and antiatherogenic therapy.
29641895	8	15	theme	lowest	1593:1598	arg1	infiltration					1611:1622	the lowest macrophage infiltration	1589:1622	the lowest macrophage infiltration	1589:1622	Furthermore, atheroprotective efficacy study in apoE-deficient mice showed that NPs-3 had the best potent efficacy, which was demonstrated by the fewest atherosclerotic lesions sizes and lipid accumulation, the lowest macrophage infiltration, and the lowest expression of monocyte chemoattractant protein-1 (MCP-1), respectively.
29641895	8	16	theme	apoE-deficient	1430:1443	arg1	mice					1445:1448	apoE-deficient mice	1430:1448	apoE-deficient mice	1430:1448	Furthermore, atheroprotective efficacy study in apoE-deficient mice showed that NPs-3 had the best potent efficacy, which was demonstrated by the fewest atherosclerotic lesions sizes and lipid accumulation, the lowest macrophage infiltration, and the lowest expression of monocyte chemoattractant protein-1 (MCP-1), respectively.
29641895	4	17	from	NCs	953:955	arg1	modification					877:888	modification	877:888	modification of higher molecular weight HA and higher HA coating density on NCs, termed as NPs-3,	877:973	The targeting mechanism was studied on the established injured endothelium-macrophage coculture system, which revealed that modification of higher molecular weight HA and higher HA coating density on NCs, termed as NPs-3, improved the intracellular uptake of nanoparticles by macrophages.
29641895	0	18	theme	Peptides/siRNA	72:85	arg1	Structure					26:34	Core-Shell Structure	15:34	Core-Shell Structure of Hyaluronic Acid/Cell-Penetrating Peptides/siRNA	15:85	Fine Tuning of Core-Shell Structure of Hyaluronic Acid/Cell-Penetrating Peptides/siRNA Nanoparticles for Enhanced Gene Delivery to Macrophages in Antiatherosclerotic Therapy.
29641895	4	19	from	density	942:948	arg1	NCs					953:955	NCs	953:955	NCs	953:955	The targeting mechanism was studied on the established injured endothelium-macrophage coculture system, which revealed that modification of higher molecular weight HA and higher HA coating density on NCs, termed as NPs-3, improved the intracellular uptake of nanoparticles by macrophages.
29641895	4	20	theme	intracellular	988:1000	arg1	uptake					1002:1007	the intracellular uptake	984:1007	the intracellular uptake of nanoparticles by macrophages	984:1039	The targeting mechanism was studied on the established injured endothelium-macrophage coculture system, which revealed that modification of higher molecular weight HA and higher HA coating density on NCs, termed as NPs-3, improved the intracellular uptake of nanoparticles by macrophages.
29641895	8	21	theme	lowest	1633:1638	arg1	expression					1640:1649	the lowest expression	1629:1649	the lowest expression of monocyte chemoattractant protein-1 (MCP-1)	1629:1695	Furthermore, atheroprotective efficacy study in apoE-deficient mice showed that NPs-3 had the best potent efficacy, which was demonstrated by the fewest atherosclerotic lesions sizes and lipid accumulation, the lowest macrophage infiltration, and the lowest expression of monocyte chemoattractant protein-1 (MCP-1), respectively.
29641895	4	22	theme	injured	808:814	arg1	system					849:854	the established injured endothelium-macrophage coculture system	792:854	the established injured endothelium-macrophage coculture system	792:854	The targeting mechanism was studied on the established injured endothelium-macrophage coculture system, which revealed that modification of higher molecular weight HA and higher HA coating density on NCs, termed as NPs-3, improved the intracellular uptake of nanoparticles by macrophages.
29641895	0	23	theme	Gene	114:117	arg1	Delivery					119:126	Enhanced Gene Delivery	105:126	Enhanced Gene Delivery to Macrophages in Antiatherosclerotic Therapy	105:172	Fine Tuning of Core-Shell Structure of Hyaluronic Acid/Cell-Penetrating Peptides/siRNA Nanoparticles for Enhanced Gene Delivery to Macrophages in Antiatherosclerotic Therapy.
29641895	8	24	from	study	1421:1425	arg1	mice					1445:1448	apoE-deficient mice	1430:1448	apoE-deficient mice	1430:1448	Furthermore, atheroprotective efficacy study in apoE-deficient mice showed that NPs-3 had the best potent efficacy, which was demonstrated by the fewest atherosclerotic lesions sizes and lipid accumulation, the lowest macrophage infiltration, and the lowest expression of monocyte chemoattractant protein-1 (MCP-1), respectively.
29641895	8	25	contain	had	1468:1470	arg2	efficacy					1488:1495	the best potent efficacy	1472:1495	the best potent efficacy	1472:1495	Furthermore, atheroprotective efficacy study in apoE-deficient mice showed that NPs-3 had the best potent efficacy, which was demonstrated by the fewest atherosclerotic lesions sizes and lipid accumulation, the lowest macrophage infiltration, and the lowest expression of monocyte chemoattractant protein-1 (MCP-1), respectively.
29641895	8	25	contain	had	1468:1470	arg1	NPs-3					1462:1466	NPs-3	1462:1466	NPs-3	1462:1466	Furthermore, atheroprotective efficacy study in apoE-deficient mice showed that NPs-3 had the best potent efficacy, which was demonstrated by the fewest atherosclerotic lesions sizes and lipid accumulation, the lowest macrophage infiltration, and the lowest expression of monocyte chemoattractant protein-1 (MCP-1), respectively.
29641895	4	26	theme	coculture	839:847	arg1	system					849:854	the established injured endothelium-macrophage coculture system	792:854	the established injured endothelium-macrophage coculture system	792:854	The targeting mechanism was studied on the established injured endothelium-macrophage coculture system, which revealed that modification of higher molecular weight HA and higher HA coating density on NCs, termed as NPs-3, improved the intracellular uptake of nanoparticles by macrophages.
29641895	2	27	theme	leaky	445:449	arg1	endothelium					451:461	leaky endothelium	445:461	leaky endothelium overexpressing CD44 receptors	445:491	The HA coating facilitated the accumulation of nanoparticles at leaky endothelium overexpressing CD44 receptors and was further degraded by hyaluronidase (HAase) intraplaques for exposing the naked CPP NCs and achieving the ultimate location into macrophages.
29641895	7	28	theme	enhanced	1288:1295	arg1	efficiency					1330:1339	enhanced atherosclerotic-lesion-targeting efficiency	1288:1339	enhanced atherosclerotic-lesion-targeting efficiency	1288:1339	Compared with NCs, HA decoration showed enhanced atherosclerotic-lesion-targeting efficiency, proven by results from ex vivo imaging.
29641895	6	29	theme	drug	1156:1159	arg1	efficacy					1161:1168	better cellular drug efficacy	1140:1168	better cellular drug efficacy	1140:1168	Moreover, NPs-3 exhibited better cellular drug efficacy in preventing macrophage-derived foam cell formation than other preparations.
29641895	8	30	theme	chemoattractant	1663:1677	arg1	MCP-1					1690:1694	MCP-1	1690:1694	MCP-1	1690:1694	Furthermore, atheroprotective efficacy study in apoE-deficient mice showed that NPs-3 had the best potent efficacy, which was demonstrated by the fewest atherosclerotic lesions sizes and lipid accumulation, the lowest macrophage infiltration, and the lowest expression of monocyte chemoattractant protein-1 (MCP-1), respectively.
29641895	8	30	theme	chemoattractant	1663:1677	arg1	protein-1					1679:1687	monocyte chemoattractant protein-1	1654:1687	monocyte chemoattractant protein-1 (MCP-1)	1654:1695	Furthermore, atheroprotective efficacy study in apoE-deficient mice showed that NPs-3 had the best potent efficacy, which was demonstrated by the fewest atherosclerotic lesions sizes and lipid accumulation, the lowest macrophage infiltration, and the lowest expression of monocyte chemoattractant protein-1 (MCP-1), respectively.
29641895	2	31	theme	nanoparticles	428:440	arg1	accumulation					412:423	the accumulation	408:423	the accumulation of nanoparticles	408:440	The HA coating facilitated the accumulation of nanoparticles at leaky endothelium overexpressing CD44 receptors and was further degraded by hyaluronidase (HAase) intraplaques for exposing the naked CPP NCs and achieving the ultimate location into macrophages.
29641895	0	32	theme	Fine	0:3	arg1	Tuning					5:10	Fine Tuning	0:10	Fine Tuning of Core-Shell Structure of Hyaluronic Acid/Cell-Penetrating Peptides/siRNA	0:85	Fine Tuning of Core-Shell Structure of Hyaluronic Acid/Cell-Penetrating Peptides/siRNA Nanoparticles for Enhanced Gene Delivery to Macrophages in Antiatherosclerotic Therapy.
29641895	1	33	theme	gene	313:316	arg1	delivery					318:325	targeted gene delivery	304:325	targeted gene delivery to macrophages	304:340	Hyaluronic-acid (HA)-coated LOX-1-specific siRNA-condensed cell-penetrating peptide (CPP) nanocomplexes (NCs) were developed for targeted gene delivery to macrophages and suppression of lipid accumulation.
29641895	7	34	dep	ex	1365:1366	arg1	vivo					1368:1371	vivo	1368:1371	vivo	1368:1371	Compared with NCs, HA decoration showed enhanced atherosclerotic-lesion-targeting efficiency, proven by results from ex vivo imaging.
29641895	6	35	theme	better	1140:1145	arg1	efficacy					1161:1168	better cellular drug efficacy	1140:1168	better cellular drug efficacy	1140:1168	Moreover, NPs-3 exhibited better cellular drug efficacy in preventing macrophage-derived foam cell formation than other preparations.
29641895	2	36	theme	HAase	536:540	arg1	intraplaques					543:554	hyaluronidase (HAase) intraplaques	521:554	hyaluronidase (HAase) intraplaques for exposing the naked CPP NCs and achieving the ultimate location into macrophages	521:638	The HA coating facilitated the accumulation of nanoparticles at leaky endothelium overexpressing CD44 receptors and was further degraded by hyaluronidase (HAase) intraplaques for exposing the naked CPP NCs and achieving the ultimate location into macrophages.
29641895	0	37	theme	Structure	26:34	arg1	Tuning					5:10	Fine Tuning	0:10	Fine Tuning of Core-Shell Structure of Hyaluronic Acid/Cell-Penetrating Peptides/siRNA	0:85	Fine Tuning of Core-Shell Structure of Hyaluronic Acid/Cell-Penetrating Peptides/siRNA Nanoparticles for Enhanced Gene Delivery to Macrophages in Antiatherosclerotic Therapy.
29641895	5	38	theme	endocytosis	1093:1103	arg1	pathway					1105:1111	caveolae-mediated endocytosis pathway	1075:1111	caveolae-mediated endocytosis pathway	1075:1111	Macrophages internalized NCs via caveolae-mediated endocytosis pathway.
29641895	2	39	theme	hyaluronidase	521:533	arg1	intraplaques					543:554	hyaluronidase (HAase) intraplaques	521:554	hyaluronidase (HAase) intraplaques for exposing the naked CPP NCs and achieving the ultimate location into macrophages	521:638	The HA coating facilitated the accumulation of nanoparticles at leaky endothelium overexpressing CD44 receptors and was further degraded by hyaluronidase (HAase) intraplaques for exposing the naked CPP NCs and achieving the ultimate location into macrophages.
29641895	3	40	theme	increased	687:695	arg1	size					706:709	the increased particle size	683:709	the increased particle size	683:709	The surface coating of HA was verified by the increased particle size, inverted zeta potential, and TEM images.
29641895	4	41	theme	higher	893:898	arg1	weight					910:915	higher molecular weight	893:915	higher molecular weight HA	893:918	The targeting mechanism was studied on the established injured endothelium-macrophage coculture system, which revealed that modification of higher molecular weight HA and higher HA coating density on NCs, termed as NPs-3, improved the intracellular uptake of nanoparticles by macrophages.
29641895	2	42	theme	HA	385:386	arg1	coating					388:394	The HA coating	381:394	The HA coating	381:394	The HA coating facilitated the accumulation of nanoparticles at leaky endothelium overexpressing CD44 receptors and was further degraded by hyaluronidase (HAase) intraplaques for exposing the naked CPP NCs and achieving the ultimate location into macrophages.
29641895	0	43	theme	Hyaluronic	39:48	arg1	Peptides/siRNA					72:85	Hyaluronic Acid/Cell-Penetrating Peptides/siRNA	39:85	Hyaluronic Acid/Cell-Penetrating Peptides/siRNA	39:85	Fine Tuning of Core-Shell Structure of Hyaluronic Acid/Cell-Penetrating Peptides/siRNA Nanoparticles for Enhanced Gene Delivery to Macrophages in Antiatherosclerotic Therapy.
29641895	1	44	theme	accumulation	367:378	arg1	suppression					346:356	suppression	346:356	suppression of lipid accumulation	346:378	Hyaluronic-acid (HA)-coated LOX-1-specific siRNA-condensed cell-penetrating peptide (CPP) nanocomplexes (NCs) were developed for targeted gene delivery to macrophages and suppression of lipid accumulation.
29641895	1	44	theme	accumulation	367:378	arg1	delivery					318:325	targeted gene delivery	304:325	targeted gene delivery to macrophages	304:340	Hyaluronic-acid (HA)-coated LOX-1-specific siRNA-condensed cell-penetrating peptide (CPP) nanocomplexes (NCs) were developed for targeted gene delivery to macrophages and suppression of lipid accumulation.
29641895	9	45	theme	antiatherogenic	1828:1842	arg1	therapy					1844:1850	antiatherogenic therapy	1828:1850	antiatherogenic therapy	1828:1850	Collectively, the HA-coated CPP NCs were promising nanocarriers for efficient macrophage-targeted gene delivery and antiatherogenic therapy.
29641895	9	46	theme	HA-coated	1730:1738	arg1	NCs					1744:1746	the HA-coated CPP NCs	1726:1746	the HA-coated CPP NCs	1726:1746	Collectively, the HA-coated CPP NCs were promising nanocarriers for efficient macrophage-targeted gene delivery and antiatherogenic therapy.
29641895	9	46	theme	HA-coated	1730:1738	arg1	nanocarriers					1763:1774	promising nanocarriers	1753:1774	promising nanocarriers for efficient macrophage-targeted gene delivery and antiatherogenic therapy	1753:1850	Collectively, the HA-coated CPP NCs were promising nanocarriers for efficient macrophage-targeted gene delivery and antiatherogenic therapy.
29641895	9	47	theme	promising	1753:1761	arg1	NCs					1744:1746	the HA-coated CPP NCs	1726:1746	the HA-coated CPP NCs	1726:1746	Collectively, the HA-coated CPP NCs were promising nanocarriers for efficient macrophage-targeted gene delivery and antiatherogenic therapy.
29641895	9	47	theme	promising	1753:1761	arg1	nanocarriers					1763:1774	promising nanocarriers	1753:1774	promising nanocarriers for efficient macrophage-targeted gene delivery and antiatherogenic therapy	1753:1850	Collectively, the HA-coated CPP NCs were promising nanocarriers for efficient macrophage-targeted gene delivery and antiatherogenic therapy.
29641895	6	48	theme	cell	1208:1211	arg1	formation					1213:1221	macrophage-derived foam cell formation	1184:1221	macrophage-derived foam cell formation	1184:1221	Moreover, NPs-3 exhibited better cellular drug efficacy in preventing macrophage-derived foam cell formation than other preparations.
29641895	6	49	link	macrophage-derived	1184:1201	arg1	formation					1213:1221	macrophage-derived foam cell formation	1184:1221	macrophage-derived foam cell formation	1184:1221	Moreover, NPs-3 exhibited better cellular drug efficacy in preventing macrophage-derived foam cell formation than other preparations.
29641895	6	50	theme	macrophage-derived	1184:1201	arg1	formation					1213:1221	macrophage-derived foam cell formation	1184:1221	macrophage-derived foam cell formation	1184:1221	Moreover, NPs-3 exhibited better cellular drug efficacy in preventing macrophage-derived foam cell formation than other preparations.
29641895	4	51	theme	higher	924:929	arg1	density					942:948	higher HA coating density	924:948	higher HA coating density on NCs, termed as NPs-3,	924:973	The targeting mechanism was studied on the established injured endothelium-macrophage coculture system, which revealed that modification of higher molecular weight HA and higher HA coating density on NCs, termed as NPs-3, improved the intracellular uptake of nanoparticles by macrophages.
29641895	7	52	theme	ex	1365:1366	arg1	imaging					1373:1379	ex vivo imaging	1365:1379	ex vivo imaging	1365:1379	Compared with NCs, HA decoration showed enhanced atherosclerotic-lesion-targeting efficiency, proven by results from ex vivo imaging.
29641895	8	53	theme	fewest	1528:1533	arg1	lesions					1551:1557	the fewest atherosclerotic lesions sizes and lipid accumulation, the lowest macrophage infiltration, and the lowest expression of monocyte chemoattractant protein-1 (MCP-1)	1524:1695	lesions	1551:1557	Furthermore, atheroprotective efficacy study in apoE-deficient mice showed that NPs-3 had the best potent efficacy, which was demonstrated by the fewest atherosclerotic lesions sizes and lipid accumulation, the lowest macrophage infiltration, and the lowest expression of monocyte chemoattractant protein-1 (MCP-1), respectively.
29641895	4	54	theme	coating	934:940	arg1	density					942:948	higher HA coating density	924:948	higher HA coating density on NCs, termed as NPs-3,	924:973	The targeting mechanism was studied on the established injured endothelium-macrophage coculture system, which revealed that modification of higher molecular weight HA and higher HA coating density on NCs, termed as NPs-3, improved the intracellular uptake of nanoparticles by macrophages.
29641895	1	55	theme	-coated	195:201	arg1	NCs					280:282	NCs	280:282	NCs	280:282	Hyaluronic-acid (HA)-coated LOX-1-specific siRNA-condensed cell-penetrating peptide (CPP) nanocomplexes (NCs) were developed for targeted gene delivery to macrophages and suppression of lipid accumulation.
29641895	1	55	theme	-coated	195:201	arg1	nanocomplexes					265:277	Hyaluronic-acid (HA)-coated LOX-1-specific siRNA-condensed cell-penetrating peptide (CPP) nanocomplexes	175:277	Hyaluronic-acid (HA)-coated LOX-1-specific siRNA-condensed cell-penetrating peptide (CPP) nanocomplexes (NCs)	175:283	Hyaluronic-acid (HA)-coated LOX-1-specific siRNA-condensed cell-penetrating peptide (CPP) nanocomplexes (NCs) were developed for targeted gene delivery to macrophages and suppression of lipid accumulation.
29641895	4	56	theme	molecular	900:908	arg1	weight					910:915	higher molecular weight	893:915	higher molecular weight HA	893:918	The targeting mechanism was studied on the established injured endothelium-macrophage coculture system, which revealed that modification of higher molecular weight HA and higher HA coating density on NCs, termed as NPs-3, improved the intracellular uptake of nanoparticles by macrophages.
29641895	1	57	theme	siRNA-condensed	218:232	arg1	CPP					260:262	CPP	260:262	CPP	260:262	Hyaluronic-acid (HA)-coated LOX-1-specific siRNA-condensed cell-penetrating peptide (CPP) nanocomplexes (NCs) were developed for targeted gene delivery to macrophages and suppression of lipid accumulation.
29641895	1	57	theme	siRNA-condensed	218:232	arg1	peptide					251:257	LOX-1-specific siRNA-condensed cell-penetrating peptide	203:257	Hyaluronic-acid (HA)-coated LOX-1-specific siRNA-condensed cell-penetrating peptide (CPP) nanocomplexes (NCs)	175:283	Hyaluronic-acid (HA)-coated LOX-1-specific siRNA-condensed cell-penetrating peptide (CPP) nanocomplexes (NCs) were developed for targeted gene delivery to macrophages and suppression of lipid accumulation.
29641895	4	58	theme	HA	917:918	arg1	modification					877:888	modification	877:888	modification of higher molecular weight HA and higher HA coating density on NCs, termed as NPs-3,	877:973	The targeting mechanism was studied on the established injured endothelium-macrophage coculture system, which revealed that modification of higher molecular weight HA and higher HA coating density on NCs, termed as NPs-3, improved the intracellular uptake of nanoparticles by macrophages.
29641895	0	59	theme	Antiatherosclerotic	146:164	arg1	Therapy					166:172	Antiatherosclerotic Therapy	146:172	Antiatherosclerotic Therapy	146:172	Fine Tuning of Core-Shell Structure of Hyaluronic Acid/Cell-Penetrating Peptides/siRNA Nanoparticles for Enhanced Gene Delivery to Macrophages in Antiatherosclerotic Therapy.
29641895	1	60	theme	peptide	251:257	arg1	NCs					280:282	NCs	280:282	NCs	280:282	Hyaluronic-acid (HA)-coated LOX-1-specific siRNA-condensed cell-penetrating peptide (CPP) nanocomplexes (NCs) were developed for targeted gene delivery to macrophages and suppression of lipid accumulation.
29641895	1	60	theme	peptide	251:257	arg1	nanocomplexes					265:277	Hyaluronic-acid (HA)-coated LOX-1-specific siRNA-condensed cell-penetrating peptide (CPP) nanocomplexes	175:277	Hyaluronic-acid (HA)-coated LOX-1-specific siRNA-condensed cell-penetrating peptide (CPP) nanocomplexes (NCs)	175:283	Hyaluronic-acid (HA)-coated LOX-1-specific siRNA-condensed cell-penetrating peptide (CPP) nanocomplexes (NCs) were developed for targeted gene delivery to macrophages and suppression of lipid accumulation.
29641895	8	61	theme	macrophage	1600:1609	arg1	infiltration					1611:1622	the lowest macrophage infiltration	1589:1622	the lowest macrophage infiltration	1589:1622	Furthermore, atheroprotective efficacy study in apoE-deficient mice showed that NPs-3 had the best potent efficacy, which was demonstrated by the fewest atherosclerotic lesions sizes and lipid accumulation, the lowest macrophage infiltration, and the lowest expression of monocyte chemoattractant protein-1 (MCP-1), respectively.
29641895	8	62	theme	efficacy	1412:1419	arg1	study					1421:1425	atheroprotective efficacy study	1395:1425	atheroprotective efficacy study in apoE-deficient mice	1395:1448	Furthermore, atheroprotective efficacy study in apoE-deficient mice showed that NPs-3 had the best potent efficacy, which was demonstrated by the fewest atherosclerotic lesions sizes and lipid accumulation, the lowest macrophage infiltration, and the lowest expression of monocyte chemoattractant protein-1 (MCP-1), respectively.
29641895	8	63	dep	lesions	1551:1557	arg1	sizes					1559:1563	sizes	1559:1563	sizes	1559:1563	Furthermore, atheroprotective efficacy study in apoE-deficient mice showed that NPs-3 had the best potent efficacy, which was demonstrated by the fewest atherosclerotic lesions sizes and lipid accumulation, the lowest macrophage infiltration, and the lowest expression of monocyte chemoattractant protein-1 (MCP-1), respectively.
29641895	4	64	theme	targeting	757:765	arg1	mechanism					767:775	The targeting mechanism	753:775	The targeting mechanism	753:775	The targeting mechanism was studied on the established injured endothelium-macrophage coculture system, which revealed that modification of higher molecular weight HA and higher HA coating density on NCs, termed as NPs-3, improved the intracellular uptake of nanoparticles by macrophages.
29641895	3	65	theme	HA	664:665	arg1	coating					653:659	The surface coating	641:659	The surface coating of HA	641:665	The surface coating of HA was verified by the increased particle size, inverted zeta potential, and TEM images.
29641895	4	66	theme	nanoparticles	1012:1024	arg1	uptake					1002:1007	the intracellular uptake	984:1007	the intracellular uptake of nanoparticles by macrophages	984:1039	The targeting mechanism was studied on the established injured endothelium-macrophage coculture system, which revealed that modification of higher molecular weight HA and higher HA coating density on NCs, termed as NPs-3, improved the intracellular uptake of nanoparticles by macrophages.
29641895	0	67	theme	Enhanced	105:112	arg1	Delivery					119:126	Enhanced Gene Delivery	105:126	Enhanced Gene Delivery to Macrophages in Antiatherosclerotic Therapy	105:172	Fine Tuning of Core-Shell Structure of Hyaluronic Acid/Cell-Penetrating Peptides/siRNA Nanoparticles for Enhanced Gene Delivery to Macrophages in Antiatherosclerotic Therapy.
29641895	7	68	theme	atherosclerotic-lesion-targeting	1297:1328	arg1	efficiency					1330:1339	enhanced atherosclerotic-lesion-targeting efficiency	1288:1339	enhanced atherosclerotic-lesion-targeting efficiency	1288:1339	Compared with NCs, HA decoration showed enhanced atherosclerotic-lesion-targeting efficiency, proven by results from ex vivo imaging.
29641895	2	69	theme	CPP	579:581	arg1	NCs					583:585	the naked CPP NCs	569:585	the naked CPP NCs	569:585	The HA coating facilitated the accumulation of nanoparticles at leaky endothelium overexpressing CD44 receptors and was further degraded by hyaluronidase (HAase) intraplaques for exposing the naked CPP NCs and achieving the ultimate location into macrophages.
29641895	8	70	theme	monocyte	1654:1661	arg1	MCP-1					1690:1694	MCP-1	1690:1694	MCP-1	1690:1694	Furthermore, atheroprotective efficacy study in apoE-deficient mice showed that NPs-3 had the best potent efficacy, which was demonstrated by the fewest atherosclerotic lesions sizes and lipid accumulation, the lowest macrophage infiltration, and the lowest expression of monocyte chemoattractant protein-1 (MCP-1), respectively.
29641895	8	70	theme	monocyte	1654:1661	arg1	protein-1					1679:1687	monocyte chemoattractant protein-1	1654:1687	monocyte chemoattractant protein-1 (MCP-1)	1654:1695	Furthermore, atheroprotective efficacy study in apoE-deficient mice showed that NPs-3 had the best potent efficacy, which was demonstrated by the fewest atherosclerotic lesions sizes and lipid accumulation, the lowest macrophage infiltration, and the lowest expression of monocyte chemoattractant protein-1 (MCP-1), respectively.
29641895	4	71	theme	established	796:806	arg1	system					849:854	the established injured endothelium-macrophage coculture system	792:854	the established injured endothelium-macrophage coculture system	792:854	The targeting mechanism was studied on the established injured endothelium-macrophage coculture system, which revealed that modification of higher molecular weight HA and higher HA coating density on NCs, termed as NPs-3, improved the intracellular uptake of nanoparticles by macrophages.
29641895	4	72	from	HA	917:918	arg1	NCs					953:955	NCs	953:955	NCs	953:955	The targeting mechanism was studied on the established injured endothelium-macrophage coculture system, which revealed that modification of higher molecular weight HA and higher HA coating density on NCs, termed as NPs-3, improved the intracellular uptake of nanoparticles by macrophages.
29641895	8	73	theme	protein-1	1679:1687	arg1	lesions					1551:1557	the fewest atherosclerotic lesions sizes and lipid accumulation, the lowest macrophage infiltration, and the lowest expression of monocyte chemoattractant protein-1 (MCP-1)	1524:1695	lesions	1551:1557	Furthermore, atheroprotective efficacy study in apoE-deficient mice showed that NPs-3 had the best potent efficacy, which was demonstrated by the fewest atherosclerotic lesions sizes and lipid accumulation, the lowest macrophage infiltration, and the lowest expression of monocyte chemoattractant protein-1 (MCP-1), respectively.
29641895	8	73	theme	protein-1	1679:1687	arg1	expression					1640:1649	the lowest expression	1629:1649	the lowest expression of monocyte chemoattractant protein-1 (MCP-1)	1629:1695	Furthermore, atheroprotective efficacy study in apoE-deficient mice showed that NPs-3 had the best potent efficacy, which was demonstrated by the fewest atherosclerotic lesions sizes and lipid accumulation, the lowest macrophage infiltration, and the lowest expression of monocyte chemoattractant protein-1 (MCP-1), respectively.
29641895	8	73	theme	protein-1	1679:1687	arg1	infiltration					1611:1622	the lowest macrophage infiltration	1589:1622	the lowest macrophage infiltration	1589:1622	Furthermore, atheroprotective efficacy study in apoE-deficient mice showed that NPs-3 had the best potent efficacy, which was demonstrated by the fewest atherosclerotic lesions sizes and lipid accumulation, the lowest macrophage infiltration, and the lowest expression of monocyte chemoattractant protein-1 (MCP-1), respectively.
29641895	8	73	theme	protein-1	1679:1687	arg1	accumulation					1575:1586	lipid accumulation	1569:1586	lipid accumulation	1569:1586	Furthermore, atheroprotective efficacy study in apoE-deficient mice showed that NPs-3 had the best potent efficacy, which was demonstrated by the fewest atherosclerotic lesions sizes and lipid accumulation, the lowest macrophage infiltration, and the lowest expression of monocyte chemoattractant protein-1 (MCP-1), respectively.
29641895	4	74	theme	endothelium-macrophage	816:837	arg1	system					849:854	the established injured endothelium-macrophage coculture system	792:854	the established injured endothelium-macrophage coculture system	792:854	The targeting mechanism was studied on the established injured endothelium-macrophage coculture system, which revealed that modification of higher molecular weight HA and higher HA coating density on NCs, termed as NPs-3, improved the intracellular uptake of nanoparticles by macrophages.
29641895	2	75	theme	naked	573:577	arg1	NCs					583:585	the naked CPP NCs	569:585	the naked CPP NCs	569:585	The HA coating facilitated the accumulation of nanoparticles at leaky endothelium overexpressing CD44 receptors and was further degraded by hyaluronidase (HAase) intraplaques for exposing the naked CPP NCs and achieving the ultimate location into macrophages.
29641895	7	76	theme	HA	1267:1268	arg1	decoration					1270:1279	HA decoration	1267:1279	HA decoration	1267:1279	Compared with NCs, HA decoration showed enhanced atherosclerotic-lesion-targeting efficiency, proven by results from ex vivo imaging.
29641895	9	77	theme	macrophage-targeted	1790:1808	arg1	delivery					1815:1822	efficient macrophage-targeted gene delivery	1780:1822	efficient macrophage-targeted gene delivery	1780:1822	Collectively, the HA-coated CPP NCs were promising nanocarriers for efficient macrophage-targeted gene delivery and antiatherogenic therapy.
29641895	6	78	theme	cellular	1147:1154	arg1	efficacy					1161:1168	better cellular drug efficacy	1140:1168	better cellular drug efficacy	1140:1168	Moreover, NPs-3 exhibited better cellular drug efficacy in preventing macrophage-derived foam cell formation than other preparations.
29641895	3	79	theme	inverted	712:719	arg1	potential					726:734	inverted zeta potential	712:734	inverted zeta potential	712:734	The surface coating of HA was verified by the increased particle size, inverted zeta potential, and TEM images.
29641895	0	80	theme	Core-Shell	15:24	arg1	Structure					26:34	Core-Shell Structure	15:34	Core-Shell Structure of Hyaluronic Acid/Cell-Penetrating Peptides/siRNA	15:85	Fine Tuning of Core-Shell Structure of Hyaluronic Acid/Cell-Penetrating Peptides/siRNA Nanoparticles for Enhanced Gene Delivery to Macrophages in Antiatherosclerotic Therapy.
29641895	8	81	theme	potent	1481:1486	arg1	efficacy					1488:1495	the best potent efficacy	1472:1495	the best potent efficacy	1472:1495	Furthermore, atheroprotective efficacy study in apoE-deficient mice showed that NPs-3 had the best potent efficacy, which was demonstrated by the fewest atherosclerotic lesions sizes and lipid accumulation, the lowest macrophage infiltration, and the lowest expression of monocyte chemoattractant protein-1 (MCP-1), respectively.
29641895	3	82	theme	particle	697:704	arg1	size					706:709	the increased particle size	683:709	the increased particle size	683:709	The surface coating of HA was verified by the increased particle size, inverted zeta potential, and TEM images.
29641895	2	83	theme	CD44	478:481	arg1	receptors					483:491	CD44 receptors	478:491	CD44 receptors	478:491	The HA coating facilitated the accumulation of nanoparticles at leaky endothelium overexpressing CD44 receptors and was further degraded by hyaluronidase (HAase) intraplaques for exposing the naked CPP NCs and achieving the ultimate location into macrophages.
29641895	5	84	theme	caveolae-mediated	1075:1091	arg1	pathway					1105:1111	caveolae-mediated endocytosis pathway	1075:1111	caveolae-mediated endocytosis pathway	1075:1111	Macrophages internalized NCs via caveolae-mediated endocytosis pathway.
29641895	0	85	theme	Acid/Cell-Penetrating	50:70	arg1	Peptides/siRNA					72:85	Hyaluronic Acid/Cell-Penetrating Peptides/siRNA	39:85	Hyaluronic Acid/Cell-Penetrating Peptides/siRNA	39:85	Fine Tuning of Core-Shell Structure of Hyaluronic Acid/Cell-Penetrating Peptides/siRNA Nanoparticles for Enhanced Gene Delivery to Macrophages in Antiatherosclerotic Therapy.
29641895	9	86	theme	CPP	1740:1742	arg1	NCs					1744:1746	the HA-coated CPP NCs	1726:1746	the HA-coated CPP NCs	1726:1746	Collectively, the HA-coated CPP NCs were promising nanocarriers for efficient macrophage-targeted gene delivery and antiatherogenic therapy.
29641895	9	86	theme	CPP	1740:1742	arg1	nanocarriers					1763:1774	promising nanocarriers	1753:1774	promising nanocarriers for efficient macrophage-targeted gene delivery and antiatherogenic therapy	1753:1850	Collectively, the HA-coated CPP NCs were promising nanocarriers for efficient macrophage-targeted gene delivery and antiatherogenic therapy.
29641895	6	87	theme	other	1228:1232	arg1	preparations					1234:1245	other preparations	1228:1245	other preparations	1228:1245	Moreover, NPs-3 exhibited better cellular drug efficacy in preventing macrophage-derived foam cell formation than other preparations.
29641895	3	88	theme	zeta	721:724	arg1	potential					726:734	inverted zeta potential	712:734	inverted zeta potential	712:734	The surface coating of HA was verified by the increased particle size, inverted zeta potential, and TEM images.
29641895	1	89	theme	lipid	361:365	arg1	accumulation					367:378	lipid accumulation	361:378	lipid accumulation	361:378	Hyaluronic-acid (HA)-coated LOX-1-specific siRNA-condensed cell-penetrating peptide (CPP) nanocomplexes (NCs) were developed for targeted gene delivery to macrophages and suppression of lipid accumulation.
29641895	8	90	theme	atherosclerotic	1535:1549	arg1	lesions					1551:1557	the fewest atherosclerotic lesions sizes and lipid accumulation, the lowest macrophage infiltration, and the lowest expression of monocyte chemoattractant protein-1 (MCP-1)	1524:1695	lesions	1551:1557	Furthermore, atheroprotective efficacy study in apoE-deficient mice showed that NPs-3 had the best potent efficacy, which was demonstrated by the fewest atherosclerotic lesions sizes and lipid accumulation, the lowest macrophage infiltration, and the lowest expression of monocyte chemoattractant protein-1 (MCP-1), respectively.
29641895	6	91	theme	foam	1203:1206	arg1	formation					1213:1221	macrophage-derived foam cell formation	1184:1221	macrophage-derived foam cell formation	1184:1221	Moreover, NPs-3 exhibited better cellular drug efficacy in preventing macrophage-derived foam cell formation than other preparations.
29641895	3	92	theme	TEM	741:743	arg1	images					745:750	TEM images	741:750	TEM images	741:750	The surface coating of HA was verified by the increased particle size, inverted zeta potential, and TEM images.
31186501	7	0	theme	molecular	1316:1324	arg1	weight					1326:1331	polymer molecular weight	1308:1331	polymer molecular weight	1308:1331	Conversely, cell-substrate adhesion was enhanced by polymers increasing the bulk viscosity of the culture medium independently of polymer molecular weight.
31186501	3	1	from	role	472:475	arg1	TME					516:518	the TME	512:518	the TME affecting endothelial cells	512:546	To study the role of extracellular macromolecules in the TME affecting endothelial cells we exposed normal and cancer-derived endothelial cells to inert polymer solutions with different physicochemical characteristics.
31186501	8	2	theme	cell	1471:1474	arg1	behaviour					1476:1484	cancer-derived endothelial cell behaviour	1444:1484	cancer-derived endothelial cell behaviour	1444:1484	These results show that the complex macromolecular composition of the extracellular fluid strongly influences cancer-derived endothelial cell behaviour, which may be crucial to understanding the role of the TME in cancer progression.
31186501	0	3	theme	cell	102:105	arg1	morphology					107:116	cell morphology	102:116	cell morphology	102:116	The extracellular fluid macromolecular composition differentially affects cell-substrate adhesion and cell morphology.
31186501	8	4	theme	endothelial	1459:1469	arg1	behaviour					1476:1484	cancer-derived endothelial cell behaviour	1444:1484	cancer-derived endothelial cell behaviour	1444:1484	These results show that the complex macromolecular composition of the extracellular fluid strongly influences cancer-derived endothelial cell behaviour, which may be crucial to understanding the role of the TME in cancer progression.
31186501	4	5	theme	cancer	682:687	arg1	SK-HEP-1					699:706	The cancer cell line SK-HEP-1	678:706	The cancer cell line SK-HEP-1	678:706	The cancer cell line SK-HEP-1, but not normal human umbilical vein endothelial cells, responded to high-macromolecular-content solutions by elongating and aligning with other cells, an effect that was molecular weight-dependent.
31186501	3	6	theme	endothelial	585:595	arg1	cells					597:601	normal and cancer-derived endothelial cells	559:601	normal and cancer-derived endothelial cells	559:601	To study the role of extracellular macromolecules in the TME affecting endothelial cells we exposed normal and cancer-derived endothelial cells to inert polymer solutions with different physicochemical characteristics.
31186501	3	7	theme	inert	606:610	arg1	solutions					620:628	inert polymer solutions	606:628	inert polymer solutions with different physicochemical characteristics	606:675	To study the role of extracellular macromolecules in the TME affecting endothelial cells we exposed normal and cancer-derived endothelial cells to inert polymer solutions with different physicochemical characteristics.
31186501	3	8	link	cancer-derived	570:583	arg1	cells					597:601	normal and cancer-derived endothelial cells	559:601	normal and cancer-derived endothelial cells	559:601	To study the role of extracellular macromolecules in the TME affecting endothelial cells we exposed normal and cancer-derived endothelial cells to inert polymer solutions with different physicochemical characteristics.
31186501	2	9	theme	cancer	309:314	arg1	progression					316:326	cancer progression	309:326	cancer progression	309:326	A fundamental step in cancer progression is the formation of a new vascular network which may originate from both pre-existing normal endothelium and cancer-derived cells.
31186501	2	10	from	step	301:304	arg1	progression					316:326	cancer progression	309:326	cancer progression	309:326	A fundamental step in cancer progression is the formation of a new vascular network which may originate from both pre-existing normal endothelium and cancer-derived cells.
31186501	7	11	theme	medium	1284:1289	arg1	viscosity					1259:1267	the bulk viscosity	1250:1267	the bulk viscosity of the culture medium independently of polymer molecular weight	1250:1331	Conversely, cell-substrate adhesion was enhanced by polymers increasing the bulk viscosity of the culture medium independently of polymer molecular weight.
31186501	5	12	theme	effects	1062:1068	arg1	induction					1043:1051	the induction	1039:1051	the induction of these effects	1039:1068	Moreover, we found that neither bulk viscosity, osmotic pressure, nor the fractional volume occupancy of polymers alone account for the induction of these effects.
31186501	4	13	theme	line	694:697	arg1	SK-HEP-1					699:706	The cancer cell line SK-HEP-1	678:706	The cancer cell line SK-HEP-1	678:706	The cancer cell line SK-HEP-1, but not normal human umbilical vein endothelial cells, responded to high-macromolecular-content solutions by elongating and aligning with other cells, an effect that was molecular weight-dependent.
31186501	4	14	theme	umbilical	730:738	arg1	cells					757:761	normal human umbilical vein endothelial cells	717:761	normal human umbilical vein endothelial cells	717:761	The cancer cell line SK-HEP-1, but not normal human umbilical vein endothelial cells, responded to high-macromolecular-content solutions by elongating and aligning with other cells, an effect that was molecular weight-dependent.
31186501	4	15	theme	normal	717:722	arg1	cells					757:761	normal human umbilical vein endothelial cells	717:761	normal human umbilical vein endothelial cells	717:761	The cancer cell line SK-HEP-1, but not normal human umbilical vein endothelial cells, responded to high-macromolecular-content solutions by elongating and aligning with other cells, an effect that was molecular weight-dependent.
31186501	8	16	link	cancer-derived	1444:1457	arg1	behaviour					1476:1484	cancer-derived endothelial cell behaviour	1444:1484	cancer-derived endothelial cell behaviour	1444:1484	These results show that the complex macromolecular composition of the extracellular fluid strongly influences cancer-derived endothelial cell behaviour, which may be crucial to understanding the role of the TME in cancer progression.
31186501	7	17	theme	cell-substrate	1190:1203	arg1	adhesion					1205:1212	cell-substrate adhesion	1190:1212	cell-substrate adhesion	1190:1212	Conversely, cell-substrate adhesion was enhanced by polymers increasing the bulk viscosity of the culture medium independently of polymer molecular weight.
31186501	8	18	theme	extracellular	1404:1416	arg1	fluid					1418:1422	the extracellular fluid	1400:1422	the extracellular fluid	1400:1422	These results show that the complex macromolecular composition of the extracellular fluid strongly influences cancer-derived endothelial cell behaviour, which may be crucial to understanding the role of the TME in cancer progression.
31186501	3	19	theme	cancer-derived	570:583	arg1	cells					597:601	normal and cancer-derived endothelial cells	559:601	normal and cancer-derived endothelial cells	559:601	To study the role of extracellular macromolecules in the TME affecting endothelial cells we exposed normal and cancer-derived endothelial cells to inert polymer solutions with different physicochemical characteristics.
31186501	8	20	from	role	1529:1532	arg1	progression					1555:1565	cancer progression	1548:1565	cancer progression	1548:1565	These results show that the complex macromolecular composition of the extracellular fluid strongly influences cancer-derived endothelial cell behaviour, which may be crucial to understanding the role of the TME in cancer progression.
31186501	1	21	theme	physical	197:204	arg1	characteristics					206:220	the physical characteristics	193:220	the physical characteristics of the extracellular fluid	193:247	Soluble macromolecules present in the tumour microenvironment (TME) alter the physical characteristics of the extracellular fluid and can affect cancer cell behaviour.
31186501	0	22	theme	extracellular	4:16	arg1	composition					39:49	The extracellular fluid macromolecular composition	0:49	The extracellular fluid macromolecular composition	0:49	The extracellular fluid macromolecular composition differentially affects cell-substrate adhesion and cell morphology.
31186501	7	23	theme	weight	1326:1331	arg1	viscosity					1259:1267	the bulk viscosity	1250:1267	the bulk viscosity of the culture medium independently of polymer molecular weight	1250:1331	Conversely, cell-substrate adhesion was enhanced by polymers increasing the bulk viscosity of the culture medium independently of polymer molecular weight.
31186501	1	24	from	microenvironment	164:179	arg1	present					142:148	present	142:148	present	142:148	Soluble macromolecules present in the tumour microenvironment (TME) alter the physical characteristics of the extracellular fluid and can affect cancer cell behaviour.
31186501	3	25	theme	polymer	612:618	arg1	solutions					620:628	inert polymer solutions	606:628	inert polymer solutions with different physicochemical characteristics	606:675	To study the role of extracellular macromolecules in the TME affecting endothelial cells we exposed normal and cancer-derived endothelial cells to inert polymer solutions with different physicochemical characteristics.
31186501	2	26	theme	pre-existing	401:412	arg1	endothelium					421:431	pre-existing normal endothelium	401:431	pre-existing normal endothelium	401:431	A fundamental step in cancer progression is the formation of a new vascular network which may originate from both pre-existing normal endothelium and cancer-derived cells.
31186501	6	27	theme	morphological	1090:1102	arg1	changes					1104:1110	these morphological changes	1084:1110	these morphological changes	1084:1110	Furthermore, these morphological changes were accompanied by an increased extracellular matrix deposition.
31186501	8	28	theme	cancer-derived	1444:1457	arg1	behaviour					1476:1484	cancer-derived endothelial cell behaviour	1444:1484	cancer-derived endothelial cell behaviour	1444:1484	These results show that the complex macromolecular composition of the extracellular fluid strongly influences cancer-derived endothelial cell behaviour, which may be crucial to understanding the role of the TME in cancer progression.
31186501	4	29	theme	high-macromolecular-content	777:803	arg1	solutions					805:813	high-macromolecular-content solutions	777:813	high-macromolecular-content solutions	777:813	The cancer cell line SK-HEP-1, but not normal human umbilical vein endothelial cells, responded to high-macromolecular-content solutions by elongating and aligning with other cells, an effect that was molecular weight-dependent.
31186501	1	30	theme	Soluble	119:125	arg1	macromolecules					127:140	Soluble macromolecules	119:140	Soluble macromolecules present in the tumour microenvironment (TME)	119:185	Soluble macromolecules present in the tumour microenvironment (TME) alter the physical characteristics of the extracellular fluid and can affect cancer cell behaviour.
31186501	8	31	theme	complex	1362:1368	arg1	composition					1385:1395	the complex macromolecular composition	1358:1395	the complex macromolecular composition of the extracellular fluid	1358:1422	These results show that the complex macromolecular composition of the extracellular fluid strongly influences cancer-derived endothelial cell behaviour, which may be crucial to understanding the role of the TME in cancer progression.
31186501	4	32	theme	human	724:728	arg1	cells					757:761	normal human umbilical vein endothelial cells	717:761	normal human umbilical vein endothelial cells	717:761	The cancer cell line SK-HEP-1, but not normal human umbilical vein endothelial cells, responded to high-macromolecular-content solutions by elongating and aligning with other cells, an effect that was molecular weight-dependent.
31186501	0	33	theme	macromolecular	24:37	arg1	composition					39:49	The extracellular fluid macromolecular composition	0:49	The extracellular fluid macromolecular composition	0:49	The extracellular fluid macromolecular composition differentially affects cell-substrate adhesion and cell morphology.
31186501	6	34	theme	matrix	1159:1164	arg1	deposition					1166:1175	an increased extracellular matrix deposition	1132:1175	an increased extracellular matrix deposition	1132:1175	Furthermore, these morphological changes were accompanied by an increased extracellular matrix deposition.
31186501	8	35	theme	fluid	1418:1422	arg1	composition					1385:1395	the complex macromolecular composition	1358:1395	the complex macromolecular composition of the extracellular fluid	1358:1422	These results show that the complex macromolecular composition of the extracellular fluid strongly influences cancer-derived endothelial cell behaviour, which may be crucial to understanding the role of the TME in cancer progression.
31186501	3	36	dep	exposed	551:557	arg1	study					462:466	study	462:466	To study the role of extracellular macromolecules in the TME affecting endothelial cells	459:546	To study the role of extracellular macromolecules in the TME affecting endothelial cells we exposed normal and cancer-derived endothelial cells to inert polymer solutions with different physicochemical characteristics.
31186501	3	37	theme	macromolecules	494:507	arg1	role					472:475	the role	468:475	the role of extracellular macromolecules in the TME affecting endothelial cells	468:546	To study the role of extracellular macromolecules in the TME affecting endothelial cells we exposed normal and cancer-derived endothelial cells to inert polymer solutions with different physicochemical characteristics.
31186501	0	38	theme	fluid	18:22	arg1	composition					39:49	The extracellular fluid macromolecular composition	0:49	The extracellular fluid macromolecular composition	0:49	The extracellular fluid macromolecular composition differentially affects cell-substrate adhesion and cell morphology.
31186501	5	39	theme	polymers	1012:1019	arg1	occupancy					999:1007	the fractional volume occupancy	977:1007	the fractional volume occupancy of polymers alone	977:1025	Moreover, we found that neither bulk viscosity, osmotic pressure, nor the fractional volume occupancy of polymers alone account for the induction of these effects.
31186501	1	40	theme	present	142:148	arg1	macromolecules					127:140	Soluble macromolecules	119:140	Soluble macromolecules present in the tumour microenvironment (TME)	119:185	Soluble macromolecules present in the tumour microenvironment (TME) alter the physical characteristics of the extracellular fluid and can affect cancer cell behaviour.
31186501	1	41	theme	extracellular	229:241	arg1	fluid					243:247	the extracellular fluid	225:247	the extracellular fluid	225:247	Soluble macromolecules present in the tumour microenvironment (TME) alter the physical characteristics of the extracellular fluid and can affect cancer cell behaviour.
31186501	3	42	theme	endothelial	530:540	arg1	cells					542:546	endothelial cells	530:546	endothelial cells	530:546	To study the role of extracellular macromolecules in the TME affecting endothelial cells we exposed normal and cancer-derived endothelial cells to inert polymer solutions with different physicochemical characteristics.
31186501	5	43	gly	occupancy	999:1007	arg2	polymers					1012:1019	polymers	1012:1019	polymers alone	1012:1025	Moreover, we found that neither bulk viscosity, osmotic pressure, nor the fractional volume occupancy of polymers alone account for the induction of these effects.
31186501	2	44	theme	cancer-derived	437:450	arg1	cells					452:456	cancer-derived cells	437:456	cancer-derived cells	437:456	A fundamental step in cancer progression is the formation of a new vascular network which may originate from both pre-existing normal endothelium and cancer-derived cells.
31186501	3	45	theme	physicochemical	645:659	arg1	characteristics					661:675	different physicochemical characteristics	635:675	different physicochemical characteristics	635:675	To study the role of extracellular macromolecules in the TME affecting endothelial cells we exposed normal and cancer-derived endothelial cells to inert polymer solutions with different physicochemical characteristics.
31186501	6	46	theme	increased	1135:1143	arg1	deposition					1166:1175	an increased extracellular matrix deposition	1132:1175	an increased extracellular matrix deposition	1132:1175	Furthermore, these morphological changes were accompanied by an increased extracellular matrix deposition.
31186501	1	47	theme	fluid	243:247	arg1	characteristics					206:220	the physical characteristics	193:220	the physical characteristics of the extracellular fluid	193:247	Soluble macromolecules present in the tumour microenvironment (TME) alter the physical characteristics of the extracellular fluid and can affect cancer cell behaviour.
31186501	6	48	theme	extracellular	1145:1157	arg1	deposition					1166:1175	an increased extracellular matrix deposition	1132:1175	an increased extracellular matrix deposition	1132:1175	Furthermore, these morphological changes were accompanied by an increased extracellular matrix deposition.
31186501	5	49	theme	bulk	939:942	arg1	viscosity					944:952	bulk viscosity	939:952	bulk viscosity	939:952	Moreover, we found that neither bulk viscosity, osmotic pressure, nor the fractional volume occupancy of polymers alone account for the induction of these effects.
31186501	5	49	theme	bulk	939:942	arg1	pressure					963:970	osmotic pressure	955:970	osmotic pressure	955:970	Moreover, we found that neither bulk viscosity, osmotic pressure, nor the fractional volume occupancy of polymers alone account for the induction of these effects.
31186501	7	50	theme	bulk	1254:1257	arg1	viscosity					1259:1267	the bulk viscosity	1250:1267	the bulk viscosity of the culture medium independently of polymer molecular weight	1250:1331	Conversely, cell-substrate adhesion was enhanced by polymers increasing the bulk viscosity of the culture medium independently of polymer molecular weight.
31186501	4	51	theme	cell	689:692	arg1	SK-HEP-1					699:706	The cancer cell line SK-HEP-1	678:706	The cancer cell line SK-HEP-1	678:706	The cancer cell line SK-HEP-1, but not normal human umbilical vein endothelial cells, responded to high-macromolecular-content solutions by elongating and aligning with other cells, an effect that was molecular weight-dependent.
31186501	3	52	theme	extracellular	480:492	arg1	macromolecules					494:507	extracellular macromolecules	480:507	extracellular macromolecules	480:507	To study the role of extracellular macromolecules in the TME affecting endothelial cells we exposed normal and cancer-derived endothelial cells to inert polymer solutions with different physicochemical characteristics.
31186501	0	53	theme	cell-substrate	74:87	arg1	adhesion					89:96	cell-substrate adhesion	74:96	cell-substrate adhesion	74:96	The extracellular fluid macromolecular composition differentially affects cell-substrate adhesion and cell morphology.
31186501	5	54	theme	osmotic	955:961	arg1	viscosity					944:952	bulk viscosity	939:952	bulk viscosity	939:952	Moreover, we found that neither bulk viscosity, osmotic pressure, nor the fractional volume occupancy of polymers alone account for the induction of these effects.
31186501	5	54	theme	osmotic	955:961	arg1	pressure					963:970	osmotic pressure	955:970	osmotic pressure	955:970	Moreover, we found that neither bulk viscosity, osmotic pressure, nor the fractional volume occupancy of polymers alone account for the induction of these effects.
31186501	2	55	theme	network	363:369	arg1	step					301:304	A fundamental step	287:304	A fundamental step in cancer progression	287:326	A fundamental step in cancer progression is the formation of a new vascular network which may originate from both pre-existing normal endothelium and cancer-derived cells.
31186501	2	55	theme	network	363:369	arg1	formation					335:343	the formation	331:343	the formation of a new vascular network which may originate from both pre-existing normal endothelium and cancer-derived cells	331:456	A fundamental step in cancer progression is the formation of a new vascular network which may originate from both pre-existing normal endothelium and cancer-derived cells.
31186501	1	56	attach	present	142:148	arg1	TME					182:184	TME	182:184	TME	182:184	Soluble macromolecules present in the tumour microenvironment (TME) alter the physical characteristics of the extracellular fluid and can affect cancer cell behaviour.
31186501	1	56	attach	present	142:148	arg2	macromolecules					127:140	Soluble macromolecules	119:140	Soluble macromolecules present in the tumour microenvironment (TME)	119:185	Soluble macromolecules present in the tumour microenvironment (TME) alter the physical characteristics of the extracellular fluid and can affect cancer cell behaviour.
31186501	1	56	attach	present	142:148	arg1	microenvironment					164:179	the tumour microenvironment	153:179	the tumour microenvironment (TME)	153:185	Soluble macromolecules present in the tumour microenvironment (TME) alter the physical characteristics of the extracellular fluid and can affect cancer cell behaviour.
31186501	2	57	link	cancer-derived	437:450	arg1	cells					452:456	cancer-derived cells	437:456	cancer-derived cells	437:456	A fundamental step in cancer progression is the formation of a new vascular network which may originate from both pre-existing normal endothelium and cancer-derived cells.
31186501	3	58	theme	different	635:643	arg1	characteristics					661:675	different physicochemical characteristics	635:675	different physicochemical characteristics	635:675	To study the role of extracellular macromolecules in the TME affecting endothelial cells we exposed normal and cancer-derived endothelial cells to inert polymer solutions with different physicochemical characteristics.
31186501	7	59	theme	polymer	1308:1314	arg1	weight					1326:1331	polymer molecular weight	1308:1331	polymer molecular weight	1308:1331	Conversely, cell-substrate adhesion was enhanced by polymers increasing the bulk viscosity of the culture medium independently of polymer molecular weight.
31186501	2	60	theme	vascular	354:361	arg1	network					363:369	a new vascular network	348:369	a new vascular network which may originate from both pre-existing normal endothelium and cancer-derived cells	348:456	A fundamental step in cancer progression is the formation of a new vascular network which may originate from both pre-existing normal endothelium and cancer-derived cells.
31186501	8	61	theme	cancer	1548:1553	arg1	progression					1555:1565	cancer progression	1548:1565	cancer progression	1548:1565	These results show that the complex macromolecular composition of the extracellular fluid strongly influences cancer-derived endothelial cell behaviour, which may be crucial to understanding the role of the TME in cancer progression.
31186501	5	62	theme	fractional	981:990	arg1	occupancy					999:1007	the fractional volume occupancy	977:1007	the fractional volume occupancy of polymers alone	977:1025	Moreover, we found that neither bulk viscosity, osmotic pressure, nor the fractional volume occupancy of polymers alone account for the induction of these effects.
31186501	2	63	theme	fundamental	289:299	arg1	step					301:304	A fundamental step	287:304	A fundamental step in cancer progression	287:326	A fundamental step in cancer progression is the formation of a new vascular network which may originate from both pre-existing normal endothelium and cancer-derived cells.
31186501	2	63	theme	fundamental	289:299	arg1	formation					335:343	the formation	331:343	the formation of a new vascular network which may originate from both pre-existing normal endothelium and cancer-derived cells	331:456	A fundamental step in cancer progression is the formation of a new vascular network which may originate from both pre-existing normal endothelium and cancer-derived cells.
31186501	4	64	theme	molecular	879:887	arg1	weight-dependent					889:904	molecular weight-dependent	879:904	molecular weight-dependent	879:904	The cancer cell line SK-HEP-1, but not normal human umbilical vein endothelial cells, responded to high-macromolecular-content solutions by elongating and aligning with other cells, an effect that was molecular weight-dependent.
31186501	4	64	theme	molecular	879:887	arg1	cells					853:857	other cells	847:857	other cells	847:857	The cancer cell line SK-HEP-1, but not normal human umbilical vein endothelial cells, responded to high-macromolecular-content solutions by elongating and aligning with other cells, an effect that was molecular weight-dependent.
31186501	4	64	theme	molecular	879:887	arg1	effect					863:868	an effect	860:868	an effect that was molecular weight-dependent	860:904	The cancer cell line SK-HEP-1, but not normal human umbilical vein endothelial cells, responded to high-macromolecular-content solutions by elongating and aligning with other cells, an effect that was molecular weight-dependent.
31186501	1	65	theme	tumour	157:162	arg1	TME					182:184	TME	182:184	TME	182:184	Soluble macromolecules present in the tumour microenvironment (TME) alter the physical characteristics of the extracellular fluid and can affect cancer cell behaviour.
31186501	1	65	theme	tumour	157:162	arg1	microenvironment					164:179	the tumour microenvironment	153:179	the tumour microenvironment (TME)	153:185	Soluble macromolecules present in the tumour microenvironment (TME) alter the physical characteristics of the extracellular fluid and can affect cancer cell behaviour.
31186501	1	66	from	present	142:148	arg1	TME					182:184	TME	182:184	TME	182:184	Soluble macromolecules present in the tumour microenvironment (TME) alter the physical characteristics of the extracellular fluid and can affect cancer cell behaviour.
31186501	1	66	from	present	142:148	arg1	microenvironment					164:179	the tumour microenvironment	153:179	the tumour microenvironment (TME)	153:185	Soluble macromolecules present in the tumour microenvironment (TME) alter the physical characteristics of the extracellular fluid and can affect cancer cell behaviour.
31186501	5	67	theme	volume	992:997	arg1	occupancy					999:1007	the fractional volume occupancy	977:1007	the fractional volume occupancy of polymers alone	977:1025	Moreover, we found that neither bulk viscosity, osmotic pressure, nor the fractional volume occupancy of polymers alone account for the induction of these effects.
31186501	4	68	theme	other	847:851	arg1	weight-dependent					889:904	molecular weight-dependent	879:904	molecular weight-dependent	879:904	The cancer cell line SK-HEP-1, but not normal human umbilical vein endothelial cells, responded to high-macromolecular-content solutions by elongating and aligning with other cells, an effect that was molecular weight-dependent.
31186501	4	68	theme	other	847:851	arg1	cells					853:857	other cells	847:857	other cells	847:857	The cancer cell line SK-HEP-1, but not normal human umbilical vein endothelial cells, responded to high-macromolecular-content solutions by elongating and aligning with other cells, an effect that was molecular weight-dependent.
31186501	4	68	theme	other	847:851	arg1	effect					863:868	an effect	860:868	an effect that was molecular weight-dependent	860:904	The cancer cell line SK-HEP-1, but not normal human umbilical vein endothelial cells, responded to high-macromolecular-content solutions by elongating and aligning with other cells, an effect that was molecular weight-dependent.
31186501	4	69	theme	vein	740:743	arg1	cells					757:761	normal human umbilical vein endothelial cells	717:761	normal human umbilical vein endothelial cells	717:761	The cancer cell line SK-HEP-1, but not normal human umbilical vein endothelial cells, responded to high-macromolecular-content solutions by elongating and aligning with other cells, an effect that was molecular weight-dependent.
31186501	2	70	theme	new	350:352	arg1	network					363:369	a new vascular network	348:369	a new vascular network which may originate from both pre-existing normal endothelium and cancer-derived cells	348:456	A fundamental step in cancer progression is the formation of a new vascular network which may originate from both pre-existing normal endothelium and cancer-derived cells.
31186501	8	71	theme	TME	1541:1543	arg1	role					1529:1532	the role	1525:1532	the role of the TME in cancer progression	1525:1565	These results show that the complex macromolecular composition of the extracellular fluid strongly influences cancer-derived endothelial cell behaviour, which may be crucial to understanding the role of the TME in cancer progression.
31186501	8	72	theme	macromolecular	1370:1383	arg1	composition					1385:1395	the complex macromolecular composition	1358:1395	the complex macromolecular composition of the extracellular fluid	1358:1422	These results show that the complex macromolecular composition of the extracellular fluid strongly influences cancer-derived endothelial cell behaviour, which may be crucial to understanding the role of the TME in cancer progression.
31186501	4	73	theme	endothelial	745:755	arg1	cells					757:761	normal human umbilical vein endothelial cells	717:761	normal human umbilical vein endothelial cells	717:761	The cancer cell line SK-HEP-1, but not normal human umbilical vein endothelial cells, responded to high-macromolecular-content solutions by elongating and aligning with other cells, an effect that was molecular weight-dependent.
31186501	2	74	theme	normal	414:419	arg1	endothelium					421:431	pre-existing normal endothelium	401:431	pre-existing normal endothelium	401:431	A fundamental step in cancer progression is the formation of a new vascular network which may originate from both pre-existing normal endothelium and cancer-derived cells.
31186501	1	75	theme	cancer	264:269	arg1	behaviour					276:284	cancer cell behaviour	264:284	cancer cell behaviour	264:284	Soluble macromolecules present in the tumour microenvironment (TME) alter the physical characteristics of the extracellular fluid and can affect cancer cell behaviour.
31186501	3	76	with	solutions	620:628	arg1	characteristics					661:675	different physicochemical characteristics	635:675	different physicochemical characteristics	635:675	To study the role of extracellular macromolecules in the TME affecting endothelial cells we exposed normal and cancer-derived endothelial cells to inert polymer solutions with different physicochemical characteristics.
31186501	3	77	theme	normal	559:564	arg1	cells					597:601	normal and cancer-derived endothelial cells	559:601	normal and cancer-derived endothelial cells	559:601	To study the role of extracellular macromolecules in the TME affecting endothelial cells we exposed normal and cancer-derived endothelial cells to inert polymer solutions with different physicochemical characteristics.
31186501	7	78	theme	culture	1276:1282	arg1	medium					1284:1289	the culture medium	1272:1289	the culture medium	1272:1289	Conversely, cell-substrate adhesion was enhanced by polymers increasing the bulk viscosity of the culture medium independently of polymer molecular weight.
31186501	1	79	theme	cell	271:274	arg1	behaviour					276:284	cancer cell behaviour	264:284	cancer cell behaviour	264:284	Soluble macromolecules present in the tumour microenvironment (TME) alter the physical characteristics of the extracellular fluid and can affect cancer cell behaviour.
30153842	6	0	theme	same	857:860	arg1	trend					862:866	the same trend	853:866	the same trend of CL species shift	853:886	Inhibition of delta 6 desaturase also resulted in the same trend of CL species shift.
30153842	10	1	theme	22-carbon	1419:1427	arg1	DHA					1429:1431	22-carbon DHA	1419:1431	22-carbon DHA (22:6)	1419:1438	CONCLUSIONS Comparisons of AA, EPA and DHA supplementation revealed that the 20-carbon EPA (20:5) and AA (20:4) triggered higher incorporation and CL remodeling efficiency than 22-carbon DHA (22:6).
30153842	10	1	theme	22-carbon	1419:1427	arg1	22:6					1434:1437	22:6	1434:1437	22:6	1434:1437	CONCLUSIONS Comparisons of AA, EPA and DHA supplementation revealed that the 20-carbon EPA (20:5) and AA (20:4) triggered higher incorporation and CL remodeling efficiency than 22-carbon DHA (22:6).
30153842	3	2	dep	line	559:562	arg1	RAW264.7					564:571	RAW264.7	564:571	the activated the macrophage cell line RAW264.7	525:571	METHODS We examined the effects of omega-6 arachidonic acid (AA), omega-3 eicosapentaenoic acid (EPA), and omega-3 docosahexaenoic acid (DHA) supplementation on the activated the macrophage cell line RAW264.7 via KdO2-lipid A (KLA).
30153842	7	3	theme	fatty	971:975	arg1	acid					977:980	polyunsaturated fatty acid	955:980	polyunsaturated fatty acid supplementation	955:996	We further examined the changes in CL and MLCL species induced by polyunsaturated fatty acid supplementation during inflammation.
30153842	7	4	from	changes	913:919	arg1	species					936:942	CL and MLCL species	924:942	CL and MLCL species induced by polyunsaturated fatty acid supplementation during inflammation	924:1016	We further examined the changes in CL and MLCL species induced by polyunsaturated fatty acid supplementation during inflammation.
30153842	0	5	theme	activated	79:87	arg1	macrophage					89:98	activated macrophage	79:98	activated macrophage	79:98	Omega-3 and omega-6 fatty acid differentially impact cardiolipin remodeling in activated macrophage.
30153842	11	6	theme	EPA	1441:1443	arg1	supplementation					1445:1459	EPA supplementation	1441:1459	EPA supplementation	1441:1459	EPA supplementation not only efficiently extended the chain length of CL but also increased the unsaturation of CL.
30153842	1	7	dep	BACKGROUND	101:110	arg1	plays					127:131	plays	127:131	plays an important role in innate immunity to induce immune responses	127:195	BACKGROUND The macrophage plays an important role in innate immunity to induce immune responses.
30153842	10	8	theme	remodeling	1392:1401	arg1	efficiency					1403:1412	CL remodeling efficiency	1389:1412	CL remodeling efficiency	1389:1412	CONCLUSIONS Comparisons of AA, EPA and DHA supplementation revealed that the 20-carbon EPA (20:5) and AA (20:4) triggered higher incorporation and CL remodeling efficiency than 22-carbon DHA (22:6).
30153842	10	9	theme	20-carbon	1319:1327	arg1	20:5					1334:1337	20:5	1334:1337	20:5	1334:1337	CONCLUSIONS Comparisons of AA, EPA and DHA supplementation revealed that the 20-carbon EPA (20:5) and AA (20:4) triggered higher incorporation and CL remodeling efficiency than 22-carbon DHA (22:6).
30153842	10	9	theme	20-carbon	1319:1327	arg1	EPA					1329:1331	the 20-carbon EPA	1315:1331	the 20-carbon EPA (20:5)	1315:1338	CONCLUSIONS Comparisons of AA, EPA and DHA supplementation revealed that the 20-carbon EPA (20:5) and AA (20:4) triggered higher incorporation and CL remodeling efficiency than 22-carbon DHA (22:6).
30153842	3	10	theme	activated	529:537	arg1	line					559:562	the macrophage cell line	539:562	the activated the macrophage cell line RAW264.7	525:571	METHODS We examined the effects of omega-6 arachidonic acid (AA), omega-3 eicosapentaenoic acid (EPA), and omega-3 docosahexaenoic acid (DHA) supplementation on the activated the macrophage cell line RAW264.7 via KdO2-lipid A (KLA).
30153842	5	11	theme	macrophage	701:710	arg1	activation					712:721	macrophage activation	701:721	macrophage activation by KLA	701:728	RESULTS After macrophage activation by KLA, CL shifted to saturated species, but did not affect the quantity of CL.
30153842	3	12	from	effects	388:394	arg1	line					559:562	the macrophage cell line	539:562	the activated the macrophage cell line RAW264.7	525:571	METHODS We examined the effects of omega-6 arachidonic acid (AA), omega-3 eicosapentaenoic acid (EPA), and omega-3 docosahexaenoic acid (DHA) supplementation on the activated the macrophage cell line RAW264.7 via KdO2-lipid A (KLA).
30153842	8	13	theme	EPA	1048:1050	arg1	supplementation					1025:1039	supplementation	1025:1039	supplementation of AA, EPA and DHA	1025:1058	After supplementation of AA, EPA and DHA, the MLCL/CL ratio increased significantly in all treatments.
30153842	11	14	theme	CL	1553:1554	arg1	unsaturation					1537:1548	the unsaturation	1533:1548	the unsaturation of CL	1533:1554	EPA supplementation not only efficiently extended the chain length of CL but also increased the unsaturation of CL.
30153842	0	15	from	impact	46:51	arg1	macrophage					89:98	activated macrophage	79:98	activated macrophage	79:98	Omega-3 and omega-6 fatty acid differentially impact cardiolipin remodeling in activated macrophage.
30153842	3	16	theme	eicosapentaenoic	438:453	arg1	EPA					461:463	EPA	461:463	EPA	461:463	METHODS We examined the effects of omega-6 arachidonic acid (AA), omega-3 eicosapentaenoic acid (EPA), and omega-3 docosahexaenoic acid (DHA) supplementation on the activated the macrophage cell line RAW264.7 via KdO2-lipid A (KLA).
30153842	3	16	theme	eicosapentaenoic	438:453	arg1	acid					455:458	omega-3 eicosapentaenoic acid	430:458	omega-3 eicosapentaenoic acid (EPA)	430:464	METHODS We examined the effects of omega-6 arachidonic acid (AA), omega-3 eicosapentaenoic acid (EPA), and omega-3 docosahexaenoic acid (DHA) supplementation on the activated the macrophage cell line RAW264.7 via KdO2-lipid A (KLA).
30153842	6	17	theme	desaturase	825:834	arg1	Inhibition					803:812	Inhibition	803:812	Inhibition of delta 6 desaturase	803:834	Inhibition of delta 6 desaturase also resulted in the same trend of CL species shift.
30153842	2	18	theme	mitochondria	275:286	arg1	compositions					259:270	the lipid compositions	249:270	the lipid compositions of mitochondria	249:286	Lipid replacement therapy has been shown to change the lipid compositions of mitochondria and potentially becomes an alternative to reduce the inflammatory response.
30153842	1	19	theme	immune	180:185	arg1	responses					187:195	immune responses	180:195	immune responses	180:195	BACKGROUND The macrophage plays an important role in innate immunity to induce immune responses.
30153842	8	20	theme	MLCL/CL	1065:1071	arg1	ratio					1073:1077	the MLCL/CL ratio	1061:1077	the MLCL/CL ratio	1061:1077	After supplementation of AA, EPA and DHA, the MLCL/CL ratio increased significantly in all treatments.
30153842	10	21	theme	higher	1364:1369	arg1	incorporation					1371:1383	higher incorporation	1364:1383	higher incorporation	1364:1383	CONCLUSIONS Comparisons of AA, EPA and DHA supplementation revealed that the 20-carbon EPA (20:5) and AA (20:4) triggered higher incorporation and CL remodeling efficiency than 22-carbon DHA (22:6).
30153842	11	22	theme	chain	1495:1499	arg1	length					1501:1506	the chain length	1491:1506	the chain length of CL	1491:1512	EPA supplementation not only efficiently extended the chain length of CL but also increased the unsaturation of CL.
30153842	3	23	theme	arachidonic	407:417	arg1	AA					425:426	AA	425:426	AA	425:426	METHODS We examined the effects of omega-6 arachidonic acid (AA), omega-3 eicosapentaenoic acid (EPA), and omega-3 docosahexaenoic acid (DHA) supplementation on the activated the macrophage cell line RAW264.7 via KdO2-lipid A (KLA).
30153842	3	23	theme	arachidonic	407:417	arg1	acid					419:422	omega-6 arachidonic acid	399:422	omega-6 arachidonic acid (AA)	399:427	METHODS We examined the effects of omega-6 arachidonic acid (AA), omega-3 eicosapentaenoic acid (EPA), and omega-3 docosahexaenoic acid (DHA) supplementation on the activated the macrophage cell line RAW264.7 via KdO2-lipid A (KLA).
30153842	9	24	theme	long-chain	1145:1154	arg1	species					1156:1162	the long-chain species	1141:1162	the long-chain species highly elevated	1141:1178	The percentages of the long-chain species highly elevated and those of short-chain species reduced in both CL and MLCL.
30153842	10	25	dep	AA	1269:1270	arg1	supplementation					1285:1299	supplementation	1285:1299	supplementation	1285:1299	CONCLUSIONS Comparisons of AA, EPA and DHA supplementation revealed that the 20-carbon EPA (20:5) and AA (20:4) triggered higher incorporation and CL remodeling efficiency than 22-carbon DHA (22:6).
30153842	3	26	theme	omega-3	430:436	arg1	EPA					461:463	EPA	461:463	EPA	461:463	METHODS We examined the effects of omega-6 arachidonic acid (AA), omega-3 eicosapentaenoic acid (EPA), and omega-3 docosahexaenoic acid (DHA) supplementation on the activated the macrophage cell line RAW264.7 via KdO2-lipid A (KLA).
30153842	3	26	theme	omega-3	430:436	arg1	acid					455:458	omega-3 eicosapentaenoic acid	430:458	omega-3 eicosapentaenoic acid (EPA)	430:464	METHODS We examined the effects of omega-6 arachidonic acid (AA), omega-3 eicosapentaenoic acid (EPA), and omega-3 docosahexaenoic acid (DHA) supplementation on the activated the macrophage cell line RAW264.7 via KdO2-lipid A (KLA).
30153842	0	27	theme	fatty	20:24	arg1	acid					26:29	omega-6 fatty acid	12:29	omega-6 fatty acid	12:29	Omega-3 and omega-6 fatty acid differentially impact cardiolipin remodeling in activated macrophage.
30153842	3	28	theme	KdO2-lipid	577:586	arg1	A					588:588	KdO2-lipid A	577:588	KdO2-lipid A (KLA)	577:594	METHODS We examined the effects of omega-6 arachidonic acid (AA), omega-3 eicosapentaenoic acid (EPA), and omega-3 docosahexaenoic acid (DHA) supplementation on the activated the macrophage cell line RAW264.7 via KdO2-lipid A (KLA).
30153842	3	28	theme	KdO2-lipid	577:586	arg1	KLA					591:593	KLA	591:593	KLA	591:593	METHODS We examined the effects of omega-6 arachidonic acid (AA), omega-3 eicosapentaenoic acid (EPA), and omega-3 docosahexaenoic acid (DHA) supplementation on the activated the macrophage cell line RAW264.7 via KdO2-lipid A (KLA).
30153842	3	29	theme	macrophage	543:552	arg1	line					559:562	the macrophage cell line	539:562	the activated the macrophage cell line RAW264.7	525:571	METHODS We examined the effects of omega-6 arachidonic acid (AA), omega-3 eicosapentaenoic acid (EPA), and omega-3 docosahexaenoic acid (DHA) supplementation on the activated the macrophage cell line RAW264.7 via KdO2-lipid A (KLA).
30153842	10	30	theme	CONCLUSIONS	1242:1252	arg1	Comparisons					1254:1264	CONCLUSIONS Comparisons	1242:1264	CONCLUSIONS Comparisons of AA, EPA and DHA supplementation	1242:1299	CONCLUSIONS Comparisons of AA, EPA and DHA supplementation revealed that the 20-carbon EPA (20:5) and AA (20:4) triggered higher incorporation and CL remodeling efficiency than 22-carbon DHA (22:6).
30153842	6	31	theme	delta	817:821	arg1	desaturase					825:834	delta 6 desaturase	817:834	delta 6 desaturase	817:834	Inhibition of delta 6 desaturase also resulted in the same trend of CL species shift.
30153842	3	32	theme	cell	554:557	arg1	line					559:562	the macrophage cell line	539:562	the activated the macrophage cell line RAW264.7	525:571	METHODS We examined the effects of omega-6 arachidonic acid (AA), omega-3 eicosapentaenoic acid (EPA), and omega-3 docosahexaenoic acid (DHA) supplementation on the activated the macrophage cell line RAW264.7 via KdO2-lipid A (KLA).
30153842	4	33	theme	mitochondrial	601:613	arg1	CL					628:629	CL	628:629	CL	628:629	The mitochondrial cardiolipin (CL) and monolysocardiolipin (MLCL) were analyzed by LC-MS.
30153842	4	33	theme	mitochondrial	601:613	arg1	cardiolipin					615:625	The mitochondrial cardiolipin	597:625	The mitochondrial cardiolipin (CL)	597:630	The mitochondrial cardiolipin (CL) and monolysocardiolipin (MLCL) were analyzed by LC-MS.
30153842	8	34	theme	AA	1044:1045	arg1	supplementation					1025:1039	supplementation	1025:1039	supplementation of AA, EPA and DHA	1025:1058	After supplementation of AA, EPA and DHA, the MLCL/CL ratio increased significantly in all treatments.
30153842	9	35	theme	elevated	1171:1178	arg1	species					1156:1162	the long-chain species	1141:1162	the long-chain species highly elevated	1141:1178	The percentages of the long-chain species highly elevated and those of short-chain species reduced in both CL and MLCL.
30153842	5	36	theme	saturated	745:753	arg1	species					755:761	saturated species	745:761	saturated species	745:761	RESULTS After macrophage activation by KLA, CL shifted to saturated species, but did not affect the quantity of CL.
30153842	6	37	theme	shift	882:886	arg1	trend					862:866	the same trend	853:866	the same trend of CL species shift	853:886	Inhibition of delta 6 desaturase also resulted in the same trend of CL species shift.
30153842	10	38	theme	CL	1389:1390	arg1	efficiency					1403:1412	CL remodeling efficiency	1389:1412	CL remodeling efficiency	1389:1412	CONCLUSIONS Comparisons of AA, EPA and DHA supplementation revealed that the 20-carbon EPA (20:5) and AA (20:4) triggered higher incorporation and CL remodeling efficiency than 22-carbon DHA (22:6).
30153842	10	39	theme	EPA	1273:1275	arg1	Comparisons					1254:1264	CONCLUSIONS Comparisons	1242:1264	CONCLUSIONS Comparisons of AA, EPA and DHA supplementation	1242:1299	CONCLUSIONS Comparisons of AA, EPA and DHA supplementation revealed that the 20-carbon EPA (20:5) and AA (20:4) triggered higher incorporation and CL remodeling efficiency than 22-carbon DHA (22:6).
30153842	5	40	theme	CL	799:800	arg1	quantity					787:794	the quantity	783:794	the quantity of CL	783:800	RESULTS After macrophage activation by KLA, CL shifted to saturated species, but did not affect the quantity of CL.
30153842	3	41	theme	acid	419:422	arg1	supplementation					506:520	omega-6 arachidonic acid (AA), omega-3 eicosapentaenoic acid (EPA), and omega-3 docosahexaenoic acid (DHA) supplementation	399:520	omega-6 arachidonic acid (AA), omega-3 eicosapentaenoic acid (EPA), and omega-3 docosahexaenoic acid (DHA) supplementation	399:520	METHODS We examined the effects of omega-6 arachidonic acid (AA), omega-3 eicosapentaenoic acid (EPA), and omega-3 docosahexaenoic acid (DHA) supplementation on the activated the macrophage cell line RAW264.7 via KdO2-lipid A (KLA).
30153842	6	42	theme	species	874:880	arg1	shift					882:886	CL species shift	871:886	CL species shift	871:886	Inhibition of delta 6 desaturase also resulted in the same trend of CL species shift.
30153842	3	43	dep	METHODS	364:370	arg1	examined					375:382	examined	375:382	examined the effects of omega-6 arachidonic acid (AA), omega-3 eicosapentaenoic acid (EPA), and omega-3 docosahexaenoic acid (DHA) supplementation on the activated the macrophage cell line RAW264.7 via KdO2-lipid A (KLA)	375:594	METHODS We examined the effects of omega-6 arachidonic acid (AA), omega-3 eicosapentaenoic acid (EPA), and omega-3 docosahexaenoic acid (DHA) supplementation on the activated the macrophage cell line RAW264.7 via KdO2-lipid A (KLA).
30153842	3	44	theme	docosahexaenoic	479:493	arg1	DHA					501:503	DHA	501:503	DHA	501:503	METHODS We examined the effects of omega-6 arachidonic acid (AA), omega-3 eicosapentaenoic acid (EPA), and omega-3 docosahexaenoic acid (DHA) supplementation on the activated the macrophage cell line RAW264.7 via KdO2-lipid A (KLA).
30153842	3	44	theme	docosahexaenoic	479:493	arg1	acid					495:498	omega-3 docosahexaenoic acid	471:498	omega-3 docosahexaenoic acid (DHA)	471:504	METHODS We examined the effects of omega-6 arachidonic acid (AA), omega-3 eicosapentaenoic acid (EPA), and omega-3 docosahexaenoic acid (DHA) supplementation on the activated the macrophage cell line RAW264.7 via KdO2-lipid A (KLA).
30153842	6	45	theme	CL	871:872	arg1	shift					882:886	CL species shift	871:886	CL species shift	871:886	Inhibition of delta 6 desaturase also resulted in the same trend of CL species shift.
30153842	7	46	theme	acid	977:980	arg1	supplementation					982:996	polyunsaturated fatty acid supplementation	955:996	polyunsaturated fatty acid supplementation	955:996	We further examined the changes in CL and MLCL species induced by polyunsaturated fatty acid supplementation during inflammation.
30153842	1	47	theme	innate	154:159	arg1	immunity					161:168	innate immunity	154:168	innate immunity to induce immune responses	154:195	BACKGROUND The macrophage plays an important role in innate immunity to induce immune responses.
30153842	10	48	theme	AA	1269:1270	arg1	Comparisons					1254:1264	CONCLUSIONS Comparisons	1242:1264	CONCLUSIONS Comparisons of AA, EPA and DHA supplementation	1242:1299	CONCLUSIONS Comparisons of AA, EPA and DHA supplementation revealed that the 20-carbon EPA (20:5) and AA (20:4) triggered higher incorporation and CL remodeling efficiency than 22-carbon DHA (22:6).
30153842	3	49	theme	acid	495:498	arg1	supplementation					506:520	omega-6 arachidonic acid (AA), omega-3 eicosapentaenoic acid (EPA), and omega-3 docosahexaenoic acid (DHA) supplementation	399:520	omega-6 arachidonic acid (AA), omega-3 eicosapentaenoic acid (EPA), and omega-3 docosahexaenoic acid (DHA) supplementation	399:520	METHODS We examined the effects of omega-6 arachidonic acid (AA), omega-3 eicosapentaenoic acid (EPA), and omega-3 docosahexaenoic acid (DHA) supplementation on the activated the macrophage cell line RAW264.7 via KdO2-lipid A (KLA).
30153842	9	50	theme	species	1156:1162	arg1	percentages					1126:1136	The percentages	1122:1136	The percentages of the long-chain species highly elevated	1122:1178	The percentages of the long-chain species highly elevated and those of short-chain species reduced in both CL and MLCL.
30153842	8	51	theme	DHA	1056:1058	arg1	supplementation					1025:1039	supplementation	1025:1039	supplementation of AA, EPA and DHA	1025:1058	After supplementation of AA, EPA and DHA, the MLCL/CL ratio increased significantly in all treatments.
30153842	9	52	theme	short-chain	1193:1203	arg1	species					1205:1211	short-chain species	1193:1211	short-chain species	1193:1211	The percentages of the long-chain species highly elevated and those of short-chain species reduced in both CL and MLCL.
30153842	7	53	theme	MLCL	931:934	arg1	species					936:942	CL and MLCL species	924:942	CL and MLCL species induced by polyunsaturated fatty acid supplementation during inflammation	924:1016	We further examined the changes in CL and MLCL species induced by polyunsaturated fatty acid supplementation during inflammation.
30153842	1	54	theme	important	136:144	arg1	role					146:149	an important role	133:149	an important role	133:149	BACKGROUND The macrophage plays an important role in innate immunity to induce immune responses.
30153842	11	55	theme	CL	1511:1512	arg1	length					1501:1506	the chain length	1491:1506	the chain length of CL	1491:1512	EPA supplementation not only efficiently extended the chain length of CL but also increased the unsaturation of CL.
30153842	7	56	theme	polyunsaturated	955:969	arg1	acid					977:980	polyunsaturated fatty acid	955:980	polyunsaturated fatty acid supplementation	955:996	We further examined the changes in CL and MLCL species induced by polyunsaturated fatty acid supplementation during inflammation.
30153842	2	57	theme	replacement	204:214	arg1	therapy					216:222	Lipid replacement therapy	198:222	Lipid replacement therapy	198:222	Lipid replacement therapy has been shown to change the lipid compositions of mitochondria and potentially becomes an alternative to reduce the inflammatory response.
30153842	3	58	theme	omega-3	471:477	arg1	DHA					501:503	DHA	501:503	DHA	501:503	METHODS We examined the effects of omega-6 arachidonic acid (AA), omega-3 eicosapentaenoic acid (EPA), and omega-3 docosahexaenoic acid (DHA) supplementation on the activated the macrophage cell line RAW264.7 via KdO2-lipid A (KLA).
30153842	3	58	theme	omega-3	471:477	arg1	acid					495:498	omega-3 docosahexaenoic acid	471:498	omega-3 docosahexaenoic acid (DHA)	471:504	METHODS We examined the effects of omega-6 arachidonic acid (AA), omega-3 eicosapentaenoic acid (EPA), and omega-3 docosahexaenoic acid (DHA) supplementation on the activated the macrophage cell line RAW264.7 via KdO2-lipid A (KLA).
30153842	2	59	theme	inflammatory	341:352	arg1	response					354:361	the inflammatory response	337:361	the inflammatory response	337:361	Lipid replacement therapy has been shown to change the lipid compositions of mitochondria and potentially becomes an alternative to reduce the inflammatory response.
30153842	2	60	theme	Lipid	198:202	arg1	therapy					216:222	Lipid replacement therapy	198:222	Lipid replacement therapy	198:222	Lipid replacement therapy has been shown to change the lipid compositions of mitochondria and potentially becomes an alternative to reduce the inflammatory response.
30153842	2	61	theme	lipid	253:257	arg1	compositions					259:270	the lipid compositions	249:270	the lipid compositions of mitochondria	249:286	Lipid replacement therapy has been shown to change the lipid compositions of mitochondria and potentially becomes an alternative to reduce the inflammatory response.
30153842	7	62	theme	CL	924:925	arg1	species					936:942	CL and MLCL species	924:942	CL and MLCL species induced by polyunsaturated fatty acid supplementation during inflammation	924:1016	We further examined the changes in CL and MLCL species induced by polyunsaturated fatty acid supplementation during inflammation.
30153842	3	63	theme	acid	455:458	arg1	supplementation					506:520	omega-6 arachidonic acid (AA), omega-3 eicosapentaenoic acid (EPA), and omega-3 docosahexaenoic acid (DHA) supplementation	399:520	omega-6 arachidonic acid (AA), omega-3 eicosapentaenoic acid (EPA), and omega-3 docosahexaenoic acid (DHA) supplementation	399:520	METHODS We examined the effects of omega-6 arachidonic acid (AA), omega-3 eicosapentaenoic acid (EPA), and omega-3 docosahexaenoic acid (DHA) supplementation on the activated the macrophage cell line RAW264.7 via KdO2-lipid A (KLA).
30153842	0	64	theme	cardiolipin	53:63	arg1	remodeling					65:74	cardiolipin remodeling	53:74	cardiolipin remodeling	53:74	Omega-3 and omega-6 fatty acid differentially impact cardiolipin remodeling in activated macrophage.
30153842	10	65	theme	DHA	1281:1283	arg1	Comparisons					1254:1264	CONCLUSIONS Comparisons	1242:1264	CONCLUSIONS Comparisons of AA, EPA and DHA supplementation	1242:1299	CONCLUSIONS Comparisons of AA, EPA and DHA supplementation revealed that the 20-carbon EPA (20:5) and AA (20:4) triggered higher incorporation and CL remodeling efficiency than 22-carbon DHA (22:6).
30153842	3	66	theme	supplementation	506:520	arg1	effects					388:394	the effects	384:394	the effects of omega-6 arachidonic acid (AA), omega-3 eicosapentaenoic acid (EPA), and omega-3 docosahexaenoic acid (DHA) supplementation on the activated the macrophage cell line RAW264.7	384:571	METHODS We examined the effects of omega-6 arachidonic acid (AA), omega-3 eicosapentaenoic acid (EPA), and omega-3 docosahexaenoic acid (DHA) supplementation on the activated the macrophage cell line RAW264.7 via KdO2-lipid A (KLA).
29414875	5	0	dep	250	864:866	arg1	to					861:862	to	861:862	to	861:862	Starting from optimized 3D structures, we evaluated both qualitatively and quantitatively the effects of static magnetic fields of up to 250 mT on cell proliferation and differentiation, by ALP (alkaline phosphatase) production, Alizarin Red and osteocalcin secretion measurements.
29414875	1	1	from	osteogenesis	180:191	arg1	fields					212:217	static magnetic fields	196:217	static magnetic fields	196:217	We designed, fabricated and optimized 3D biomimetic magnetic structures that stimulate the osteogenesis in static magnetic fields.
29414875	3	2	theme	composite	558:566	arg1	layer					493:497	a thin layer	486:497	a thin layer of collagen-chitosan-hydroxyapatite-magnetic nanoparticles composite	486:566	The magnetic activity of the structures was assured by coating with a thin layer of collagen-chitosan-hydroxyapatite-magnetic nanoparticles composite.
29414875	0	3	theme	Osteogenesis	75:86	arg1	Stimulation					60:70	Static Magnetic Field Stimulation	38:70	Static Magnetic Field Stimulation of Osteogenesis	38:86	3D Biomimetic Magnetic Structures for Static Magnetic Field Stimulation of Osteogenesis.
29414875	5	4	theme	fields	848:853	arg1	effects					821:827	the effects	817:827	the effects of static magnetic fields of up to 250 mT on cell proliferation and differentiation	817:911	Starting from optimized 3D structures, we evaluated both qualitatively and quantitatively the effects of static magnetic fields of up to 250 mT on cell proliferation and differentiation, by ALP (alkaline phosphatase) production, Alizarin Red and osteocalcin secretion measurements.
29414875	4	5	theme	In	569:570	arg1	experiments					578:588	In vitro experiments	569:588	In vitro experiments using MG-63 osteoblast-like cells for 3D structures with gradients of pore size	569:668	In vitro experiments using MG-63 osteoblast-like cells for 3D structures with gradients of pore size helped us to find an optimum pore size between 20-40 µm.
29414875	4	6	with	structures	631:640	arg1	gradients					647:655	gradients	647:655	gradients of pore size	647:668	In vitro experiments using MG-63 osteoblast-like cells for 3D structures with gradients of pore size helped us to find an optimum pore size between 20-40 µm.
29414875	4	7	theme	pore	660:663	arg1	size					665:668	pore size	660:668	pore size	660:668	In vitro experiments using MG-63 osteoblast-like cells for 3D structures with gradients of pore size helped us to find an optimum pore size between 20-40 µm.
29414875	5	8	theme	Red	965:967	arg1	Alizarin					956:963	Alizarin Red	956:967	Alizarin Red	956:967	Starting from optimized 3D structures, we evaluated both qualitatively and quantitatively the effects of static magnetic fields of up to 250 mT on cell proliferation and differentiation, by ALP (alkaline phosphatase) production, Alizarin Red and osteocalcin secretion measurements.
29414875	5	9	theme	cell	874:877	arg1	proliferation					879:891	cell proliferation	874:891	cell proliferation	874:891	Starting from optimized 3D structures, we evaluated both qualitatively and quantitatively the effects of static magnetic fields of up to 250 mT on cell proliferation and differentiation, by ALP (alkaline phosphatase) production, Alizarin Red and osteocalcin secretion measurements.
29414875	6	10	theme	bone	1124:1127	arg1	regeneration					1129:1140	the bone regeneration	1120:1140	the bone regeneration	1120:1140	We demonstrated that the synergic effect of 3D structure optimization and static magnetic stimulation enhances the bone regeneration by a factor greater than 2 as compared with the same structure in the absence of a magnetic field.
29414875	6	11	theme	stimulation	1099:1109	arg1	effect					1043:1048	the synergic effect	1030:1048	the synergic effect of 3D structure optimization and static magnetic stimulation	1030:1109	We demonstrated that the synergic effect of 3D structure optimization and static magnetic stimulation enhances the bone regeneration by a factor greater than 2 as compared with the same structure in the absence of a magnetic field.
29414875	5	12	theme	static	832:837	arg1	fields					848:853	static magnetic fields	832:853	static magnetic fields of up to 250 mT	832:869	Starting from optimized 3D structures, we evaluated both qualitatively and quantitatively the effects of static magnetic fields of up to 250 mT on cell proliferation and differentiation, by ALP (alkaline phosphatase) production, Alizarin Red and osteocalcin secretion measurements.
29414875	2	13	theme	two-photon	279:288	arg1	polymerization					290:303	two-photon polymerization	279:303	two-photon polymerization of IP-L780 photopolymer	279:327	The structures were fabricated by direct laser writing via two-photon polymerization of IP-L780 photopolymer and were based on ellipsoidal, hexagonal units organized in a multilayered architecture.
29414875	0	14	theme	Biomimetic	3:12	arg1	Structures					23:32	3D Biomimetic Magnetic Structures	0:32	3D Biomimetic Magnetic Structures	0:32	3D Biomimetic Magnetic Structures for Static Magnetic Field Stimulation of Osteogenesis.
29414875	2	15	dep	ellipsoidal	347:357	arg1	hexagonal					360:368	hexagonal	360:368	hexagonal	360:368	The structures were fabricated by direct laser writing via two-photon polymerization of IP-L780 photopolymer and were based on ellipsoidal, hexagonal units organized in a multilayered architecture.
29414875	3	16	theme	structures	447:456	arg1	activity					431:438	The magnetic activity	418:438	The magnetic activity of the structures	418:456	The magnetic activity of the structures was assured by coating with a thin layer of collagen-chitosan-hydroxyapatite-magnetic nanoparticles composite.
29414875	0	17	theme	3D	0:1	arg1	Structures					23:32	3D Biomimetic Magnetic Structures	0:32	3D Biomimetic Magnetic Structures	0:32	3D Biomimetic Magnetic Structures for Static Magnetic Field Stimulation of Osteogenesis.
29414875	2	18	theme	multilayered	391:402	arg1	architecture					404:415	a multilayered architecture	389:415	a multilayered architecture	389:415	The structures were fabricated by direct laser writing via two-photon polymerization of IP-L780 photopolymer and were based on ellipsoidal, hexagonal units organized in a multilayered architecture.
29414875	4	19	theme	size	665:668	arg1	gradients					647:655	gradients	647:655	gradients of pore size	647:668	In vitro experiments using MG-63 osteoblast-like cells for 3D structures with gradients of pore size helped us to find an optimum pore size between 20-40 µm.
29414875	5	20	theme	3D	751:752	arg1	structures					754:763	optimized 3D structures	741:763	optimized 3D structures	741:763	Starting from optimized 3D structures, we evaluated both qualitatively and quantitatively the effects of static magnetic fields of up to 250 mT on cell proliferation and differentiation, by ALP (alkaline phosphatase) production, Alizarin Red and osteocalcin secretion measurements.
29414875	5	21	theme	secretion	985:993	arg1	measurements					995:1006	secretion measurements	985:1006	secretion measurements	985:1006	Starting from optimized 3D structures, we evaluated both qualitatively and quantitatively the effects of static magnetic fields of up to 250 mT on cell proliferation and differentiation, by ALP (alkaline phosphatase) production, Alizarin Red and osteocalcin secretion measurements.
29414875	1	22	theme	magnetic	203:210	arg1	fields					212:217	static magnetic fields	196:217	static magnetic fields	196:217	We designed, fabricated and optimized 3D biomimetic magnetic structures that stimulate the osteogenesis in static magnetic fields.
29414875	0	23	theme	Magnetic	14:21	arg1	Structures					23:32	3D Biomimetic Magnetic Structures	0:32	3D Biomimetic Magnetic Structures	0:32	3D Biomimetic Magnetic Structures for Static Magnetic Field Stimulation of Osteogenesis.
29414875	6	24	theme	magnetic	1225:1232	arg1	field					1234:1238	a magnetic field	1223:1238	a magnetic field	1223:1238	We demonstrated that the synergic effect of 3D structure optimization and static magnetic stimulation enhances the bone regeneration by a factor greater than 2 as compared with the same structure in the absence of a magnetic field.
29414875	2	25	theme	photopolymer	316:327	arg1	polymerization					290:303	two-photon polymerization	279:303	two-photon polymerization of IP-L780 photopolymer	279:327	The structures were fabricated by direct laser writing via two-photon polymerization of IP-L780 photopolymer and were based on ellipsoidal, hexagonal units organized in a multilayered architecture.
29414875	6	26	from	structure	1195:1203	arg1	absence					1212:1218	the absence	1208:1218	the absence of a magnetic field	1208:1238	We demonstrated that the synergic effect of 3D structure optimization and static magnetic stimulation enhances the bone regeneration by a factor greater than 2 as compared with the same structure in the absence of a magnetic field.
29414875	3	27	theme	thin	488:491	arg1	layer					493:497	a thin layer	486:497	a thin layer of collagen-chitosan-hydroxyapatite-magnetic nanoparticles composite	486:566	The magnetic activity of the structures was assured by coating with a thin layer of collagen-chitosan-hydroxyapatite-magnetic nanoparticles composite.
29414875	0	28	theme	Static	38:43	arg1	Stimulation					60:70	Static Magnetic Field Stimulation	38:70	Static Magnetic Field Stimulation of Osteogenesis	38:86	3D Biomimetic Magnetic Structures for Static Magnetic Field Stimulation of Osteogenesis.
29414875	4	29	theme	3D	628:629	arg1	structures					631:640	3D structures	628:640	3D structures with gradients of pore size	628:668	In vitro experiments using MG-63 osteoblast-like cells for 3D structures with gradients of pore size helped us to find an optimum pore size between 20-40 µm.
29414875	6	30	theme	magnetic	1090:1097	arg1	stimulation					1099:1109	static magnetic stimulation	1083:1109	static magnetic stimulation	1083:1109	We demonstrated that the synergic effect of 3D structure optimization and static magnetic stimulation enhances the bone regeneration by a factor greater than 2 as compared with the same structure in the absence of a magnetic field.
29414875	1	31	theme	static	196:201	arg1	fields					212:217	static magnetic fields	196:217	static magnetic fields	196:217	We designed, fabricated and optimized 3D biomimetic magnetic structures that stimulate the osteogenesis in static magnetic fields.
29414875	4	32	theme	pore	699:702	arg1	size					704:707	an optimum pore size	688:707	an optimum pore size between 20-40 µm	688:724	In vitro experiments using MG-63 osteoblast-like cells for 3D structures with gradients of pore size helped us to find an optimum pore size between 20-40 µm.
29414875	5	33	theme	magnetic	839:846	arg1	fields					848:853	static magnetic fields	832:853	static magnetic fields of up to 250 mT	832:869	Starting from optimized 3D structures, we evaluated both qualitatively and quantitatively the effects of static magnetic fields of up to 250 mT on cell proliferation and differentiation, by ALP (alkaline phosphatase) production, Alizarin Red and osteocalcin secretion measurements.
29414875	5	34	dep	ALP	917:919	arg1	measurements					995:1006	secretion measurements	985:1006	secretion measurements	985:1006	Starting from optimized 3D structures, we evaluated both qualitatively and quantitatively the effects of static magnetic fields of up to 250 mT on cell proliferation and differentiation, by ALP (alkaline phosphatase) production, Alizarin Red and osteocalcin secretion measurements.
29414875	4	35	theme	optimum	691:697	arg1	size					704:707	an optimum pore size	688:707	an optimum pore size between 20-40 µm	688:724	In vitro experiments using MG-63 osteoblast-like cells for 3D structures with gradients of pore size helped us to find an optimum pore size between 20-40 µm.
29414875	0	36	theme	Field	54:58	arg1	Stimulation					60:70	Static Magnetic Field Stimulation	38:70	Static Magnetic Field Stimulation of Osteogenesis	38:86	3D Biomimetic Magnetic Structures for Static Magnetic Field Stimulation of Osteogenesis.
29414875	4	37	dep	In	569:570	arg1	vitro					572:576	vitro	572:576	vitro	572:576	In vitro experiments using MG-63 osteoblast-like cells for 3D structures with gradients of pore size helped us to find an optimum pore size between 20-40 µm.
29414875	3	38	theme	collagen-chitosan-hydroxyapatite-magnetic	502:542	arg1	composite					558:566	collagen-chitosan-hydroxyapatite-magnetic nanoparticles composite	502:566	collagen-chitosan-hydroxyapatite-magnetic nanoparticles composite	502:566	The magnetic activity of the structures was assured by coating with a thin layer of collagen-chitosan-hydroxyapatite-magnetic nanoparticles composite.
29414875	0	39	theme	Magnetic	45:52	arg1	Stimulation					60:70	Static Magnetic Field Stimulation	38:70	Static Magnetic Field Stimulation of Osteogenesis	38:86	3D Biomimetic Magnetic Structures for Static Magnetic Field Stimulation of Osteogenesis.
29414875	4	40	theme	osteoblast-like	602:616	arg1	cells					618:622	MG-63 osteoblast-like cells	596:622	MG-63 osteoblast-like cells for 3D structures with gradients of pore size	596:668	In vitro experiments using MG-63 osteoblast-like cells for 3D structures with gradients of pore size helped us to find an optimum pore size between 20-40 µm.
29414875	5	41	from	effects	821:827	arg1	proliferation					879:891	cell proliferation	874:891	cell proliferation	874:891	Starting from optimized 3D structures, we evaluated both qualitatively and quantitatively the effects of static magnetic fields of up to 250 mT on cell proliferation and differentiation, by ALP (alkaline phosphatase) production, Alizarin Red and osteocalcin secretion measurements.
29414875	5	41	from	effects	821:827	arg1	differentiation					897:911	differentiation	897:911	differentiation	897:911	Starting from optimized 3D structures, we evaluated both qualitatively and quantitatively the effects of static magnetic fields of up to 250 mT on cell proliferation and differentiation, by ALP (alkaline phosphatase) production, Alizarin Red and osteocalcin secretion measurements.
29414875	3	42	theme	nanoparticles	544:556	arg1	composite					558:566	collagen-chitosan-hydroxyapatite-magnetic nanoparticles composite	502:566	collagen-chitosan-hydroxyapatite-magnetic nanoparticles composite	502:566	The magnetic activity of the structures was assured by coating with a thin layer of collagen-chitosan-hydroxyapatite-magnetic nanoparticles composite.
29414875	4	43	theme	MG-63	596:600	arg1	cells					618:622	MG-63 osteoblast-like cells	596:622	MG-63 osteoblast-like cells for 3D structures with gradients of pore size	596:668	In vitro experiments using MG-63 osteoblast-like cells for 3D structures with gradients of pore size helped us to find an optimum pore size between 20-40 µm.
29414875	0	44	dep	Structures	23:32	arg1	Stimulation					60:70	Static Magnetic Field Stimulation	38:70	Static Magnetic Field Stimulation of Osteogenesis	38:86	3D Biomimetic Magnetic Structures for Static Magnetic Field Stimulation of Osteogenesis.
29414875	6	45	theme	optimization	1066:1077	arg1	effect					1043:1048	the synergic effect	1030:1048	the synergic effect of 3D structure optimization and static magnetic stimulation	1030:1109	We demonstrated that the synergic effect of 3D structure optimization and static magnetic stimulation enhances the bone regeneration by a factor greater than 2 as compared with the same structure in the absence of a magnetic field.
29414875	1	46	theme	3D	127:128	arg1	structures					150:159	3D biomimetic magnetic structures	127:159	3D biomimetic magnetic structures that stimulate the osteogenesis in static magnetic fields	127:217	We designed, fabricated and optimized 3D biomimetic magnetic structures that stimulate the osteogenesis in static magnetic fields.
29414875	6	47	theme	same	1190:1193	arg1	structure					1195:1203	the same structure	1186:1203	the same structure in the absence of a magnetic field	1186:1238	We demonstrated that the synergic effect of 3D structure optimization and static magnetic stimulation enhances the bone regeneration by a factor greater than 2 as compared with the same structure in the absence of a magnetic field.
29414875	6	48	theme	synergic	1034:1041	arg1	effect					1043:1048	the synergic effect	1030:1048	the synergic effect of 3D structure optimization and static magnetic stimulation	1030:1109	We demonstrated that the synergic effect of 3D structure optimization and static magnetic stimulation enhances the bone regeneration by a factor greater than 2 as compared with the same structure in the absence of a magnetic field.
29414875	5	49	theme	optimized	741:749	arg1	structures					754:763	optimized 3D structures	741:763	optimized 3D structures	741:763	Starting from optimized 3D structures, we evaluated both qualitatively and quantitatively the effects of static magnetic fields of up to 250 mT on cell proliferation and differentiation, by ALP (alkaline phosphatase) production, Alizarin Red and osteocalcin secretion measurements.
29414875	5	50	theme	mT	868:869	arg1	fields					848:853	static magnetic fields	832:853	static magnetic fields of up to 250 mT	832:869	Starting from optimized 3D structures, we evaluated both qualitatively and quantitatively the effects of static magnetic fields of up to 250 mT on cell proliferation and differentiation, by ALP (alkaline phosphatase) production, Alizarin Red and osteocalcin secretion measurements.
29414875	1	51	theme	biomimetic	130:139	arg1	structures					150:159	3D biomimetic magnetic structures	127:159	3D biomimetic magnetic structures that stimulate the osteogenesis in static magnetic fields	127:217	We designed, fabricated and optimized 3D biomimetic magnetic structures that stimulate the osteogenesis in static magnetic fields.
29414875	2	52	theme	ellipsoidal	347:357	arg1	units					370:374	ellipsoidal, hexagonal units	347:374	ellipsoidal, hexagonal units organized in a multilayered architecture	347:415	The structures were fabricated by direct laser writing via two-photon polymerization of IP-L780 photopolymer and were based on ellipsoidal, hexagonal units organized in a multilayered architecture.
29414875	6	53	theme	static	1083:1088	arg1	stimulation					1099:1109	static magnetic stimulation	1083:1109	static magnetic stimulation	1083:1109	We demonstrated that the synergic effect of 3D structure optimization and static magnetic stimulation enhances the bone regeneration by a factor greater than 2 as compared with the same structure in the absence of a magnetic field.
29414875	6	54	theme	field	1234:1238	arg1	absence					1212:1218	the absence	1208:1218	the absence of a magnetic field	1208:1238	We demonstrated that the synergic effect of 3D structure optimization and static magnetic stimulation enhances the bone regeneration by a factor greater than 2 as compared with the same structure in the absence of a magnetic field.
29414875	1	55	theme	magnetic	141:148	arg1	structures					150:159	3D biomimetic magnetic structures	127:159	3D biomimetic magnetic structures that stimulate the osteogenesis in static magnetic fields	127:217	We designed, fabricated and optimized 3D biomimetic magnetic structures that stimulate the osteogenesis in static magnetic fields.
29414875	6	56	theme	structure	1056:1064	arg1	optimization					1066:1077	3D structure optimization	1053:1077	3D structure optimization	1053:1077	We demonstrated that the synergic effect of 3D structure optimization and static magnetic stimulation enhances the bone regeneration by a factor greater than 2 as compared with the same structure in the absence of a magnetic field.
29414875	3	57	theme	magnetic	422:429	arg1	activity					431:438	The magnetic activity	418:438	The magnetic activity of the structures	418:456	The magnetic activity of the structures was assured by coating with a thin layer of collagen-chitosan-hydroxyapatite-magnetic nanoparticles composite.
29414875	2	58	theme	direct	254:259	arg1	laser					261:265	direct laser	254:265	direct laser writing via two-photon polymerization of IP-L780 photopolymer	254:327	The structures were fabricated by direct laser writing via two-photon polymerization of IP-L780 photopolymer and were based on ellipsoidal, hexagonal units organized in a multilayered architecture.
29414875	5	59	theme	alkaline	922:929	arg1	phosphatase					931:941	alkaline phosphatase	922:941	alkaline phosphatase	922:941	Starting from optimized 3D structures, we evaluated both qualitatively and quantitatively the effects of static magnetic fields of up to 250 mT on cell proliferation and differentiation, by ALP (alkaline phosphatase) production, Alizarin Red and osteocalcin secretion measurements.
29414875	5	59	theme	alkaline	922:929	arg1	ALP					917:919	ALP	917:919	ALP	917:919	Starting from optimized 3D structures, we evaluated both qualitatively and quantitatively the effects of static magnetic fields of up to 250 mT on cell proliferation and differentiation, by ALP (alkaline phosphatase) production, Alizarin Red and osteocalcin secretion measurements.
29414875	6	60	theme	3D	1053:1054	arg1	optimization					1066:1077	3D structure optimization	1053:1077	3D structure optimization	1053:1077	We demonstrated that the synergic effect of 3D structure optimization and static magnetic stimulation enhances the bone regeneration by a factor greater than 2 as compared with the same structure in the absence of a magnetic field.
30715842	0	0	theme	Bone	88:91	arg1	Repair					93:98	Bone Repair	88:98	Bone Repair	88:98	Electrophoretic Deposition of Bioadaptive Drug Delivery Coatings on Magnesium Alloy for Bone Repair.
30715842	6	1	theme	genes	964:968	arg1	mineralization					1054:1067	extracellular matrix mineralization	1033:1067	extracellular matrix mineralization	1033:1067	Additionally, the osteogenic differentiation of MC3T3-E1 cells on SIM-containing coatings was assessed by measuring the expression of osteogenic genes and related proteins, alkaline phosphatase (ALP) activity, and extracellular matrix mineralization, showing that the SIM-loaded composite coating could upregulate the expression of osteogenic genes and related proteins, promote ALP activity, and enhance extracellular matrix mineralization.
30715842	6	1	theme	genes	964:968	arg1	activity					1019:1026	alkaline phosphatase (ALP) activity	992:1026	alkaline phosphatase (ALP) activity	992:1026	Additionally, the osteogenic differentiation of MC3T3-E1 cells on SIM-containing coatings was assessed by measuring the expression of osteogenic genes and related proteins, alkaline phosphatase (ALP) activity, and extracellular matrix mineralization, showing that the SIM-loaded composite coating could upregulate the expression of osteogenic genes and related proteins, promote ALP activity, and enhance extracellular matrix mineralization.
30715842	6	1	theme	genes	964:968	arg1	expression					939:948	the expression	935:948	the expression of osteogenic genes and related proteins	935:989	Additionally, the osteogenic differentiation of MC3T3-E1 cells on SIM-containing coatings was assessed by measuring the expression of osteogenic genes and related proteins, alkaline phosphatase (ALP) activity, and extracellular matrix mineralization, showing that the SIM-loaded composite coating could upregulate the expression of osteogenic genes and related proteins, promote ALP activity, and enhance extracellular matrix mineralization.
30715842	6	2	theme	SIM-loaded	1087:1096	arg1	coating					1108:1114	the SIM-loaded composite coating	1083:1114	the SIM-loaded composite coating	1083:1114	Additionally, the osteogenic differentiation of MC3T3-E1 cells on SIM-containing coatings was assessed by measuring the expression of osteogenic genes and related proteins, alkaline phosphatase (ALP) activity, and extracellular matrix mineralization, showing that the SIM-loaded composite coating could upregulate the expression of osteogenic genes and related proteins, promote ALP activity, and enhance extracellular matrix mineralization.
30715842	4	3	theme	bare	712:715	arg1	WE43					717:720	contrast to bare WE43	700:720	contrast to bare WE43	700:720	Both the degradation and corrosion rates of the coated substrate were significantly minimized in contrast to bare WE43.
30715842	6	4	theme	extracellular	1033:1045	arg1	mineralization					1054:1067	extracellular matrix mineralization	1033:1067	extracellular matrix mineralization	1033:1067	Additionally, the osteogenic differentiation of MC3T3-E1 cells on SIM-containing coatings was assessed by measuring the expression of osteogenic genes and related proteins, alkaline phosphatase (ALP) activity, and extracellular matrix mineralization, showing that the SIM-loaded composite coating could upregulate the expression of osteogenic genes and related proteins, promote ALP activity, and enhance extracellular matrix mineralization.
30715842	6	5	theme	osteogenic	953:962	arg1	genes					964:968	osteogenic genes	953:968	osteogenic genes	953:968	Additionally, the osteogenic differentiation of MC3T3-E1 cells on SIM-containing coatings was assessed by measuring the expression of osteogenic genes and related proteins, alkaline phosphatase (ALP) activity, and extracellular matrix mineralization, showing that the SIM-loaded composite coating could upregulate the expression of osteogenic genes and related proteins, promote ALP activity, and enhance extracellular matrix mineralization.
30715842	6	6	theme	osteogenic	1151:1160	arg1	genes					1162:1166	osteogenic genes	1151:1166	osteogenic genes	1151:1166	Additionally, the osteogenic differentiation of MC3T3-E1 cells on SIM-containing coatings was assessed by measuring the expression of osteogenic genes and related proteins, alkaline phosphatase (ALP) activity, and extracellular matrix mineralization, showing that the SIM-loaded composite coating could upregulate the expression of osteogenic genes and related proteins, promote ALP activity, and enhance extracellular matrix mineralization.
30715842	2	7	theme	WE43	353:356	arg1	substrate					358:366	WE43 substrate	353:366	WE43 substrate	353:366	A gelatin nanospheres/chitosan (GNs/CTS) composite coating on WE43 substrate was fabricated by electrophoretic deposition with simvastatin (SIM) loaded into the GNs.
30715842	3	8	theme	WE43	547:550	arg1	substrates					552:561	the coated WE43 substrates	536:561	the coated WE43 substrates	536:561	Apart from a sustained drug release over 28 days, an anticorrosion behavior of the coated WE43 substrates was confirmed by electrochemical tests.
30715842	7	9	theme	composite	1296:1304	arg1	able					1320:1323	able	1320:1323	able	1320:1323	In summary, the SIM-loaded GNs/CTS composite coatings were able to enhance the corrosion resistance of the WE43 substrate and promote osteogenic activity, thus demonstrating a promising coating system for modifying the surface of magnesium alloys targeted for orthopedic applications.
30715842	7	9	theme	composite	1296:1304	arg1	coatings					1306:1313	the SIM-loaded GNs/CTS composite coatings	1273:1313	the SIM-loaded GNs/CTS composite coatings	1273:1313	In summary, the SIM-loaded GNs/CTS composite coatings were able to enhance the corrosion resistance of the WE43 substrate and promote osteogenic activity, thus demonstrating a promising coating system for modifying the surface of magnesium alloys targeted for orthopedic applications.
30715842	1	10	dep	delivery	281:288	arg1	e.g.					270:273	e.g.	270:273	e.g.	270:273	Biodegradable polymer coatings on magnesium alloys are attractive, as they can provide corrosion resistance as well as additional functions for biomedical applications, e.g., drug delivery.
30715842	6	11	theme	osteogenic	837:846	arg1	differentiation					848:862	the osteogenic differentiation	833:862	the osteogenic differentiation of MC3T3-E1 cells on SIM-containing coatings	833:907	Additionally, the osteogenic differentiation of MC3T3-E1 cells on SIM-containing coatings was assessed by measuring the expression of osteogenic genes and related proteins, alkaline phosphatase (ALP) activity, and extracellular matrix mineralization, showing that the SIM-loaded composite coating could upregulate the expression of osteogenic genes and related proteins, promote ALP activity, and enhance extracellular matrix mineralization.
30715842	6	12	theme	matrix	1238:1243	arg1	mineralization					1245:1258	extracellular matrix mineralization	1224:1258	extracellular matrix mineralization	1224:1258	Additionally, the osteogenic differentiation of MC3T3-E1 cells on SIM-containing coatings was assessed by measuring the expression of osteogenic genes and related proteins, alkaline phosphatase (ALP) activity, and extracellular matrix mineralization, showing that the SIM-loaded composite coating could upregulate the expression of osteogenic genes and related proteins, promote ALP activity, and enhance extracellular matrix mineralization.
30715842	2	13	theme	gelatin	293:299	arg1	coating					342:348	A gelatin nanospheres/chitosan (GNs/CTS) composite coating	291:348	A gelatin nanospheres/chitosan (GNs/CTS) composite coating on WE43 substrate	291:366	A gelatin nanospheres/chitosan (GNs/CTS) composite coating on WE43 substrate was fabricated by electrophoretic deposition with simvastatin (SIM) loaded into the GNs.
30715842	3	14	theme	substrates	552:561	arg1	behavior					524:531	 an anticorrosion behavior	506:531	 an anticorrosion behavior of the coated WE43 substrates	506:561	Apart from a sustained drug release over 28 days, an anticorrosion behavior of the coated WE43 substrates was confirmed by electrochemical tests.
30715842	3	15	theme	coated	540:545	arg1	substrates					552:561	the coated WE43 substrates	536:561	the coated WE43 substrates	536:561	Apart from a sustained drug release over 28 days, an anticorrosion behavior of the coated WE43 substrates was confirmed by electrochemical tests.
30715842	6	16	theme	extracellular	1224:1236	arg1	mineralization					1245:1258	extracellular matrix mineralization	1224:1258	extracellular matrix mineralization	1224:1258	Additionally, the osteogenic differentiation of MC3T3-E1 cells on SIM-containing coatings was assessed by measuring the expression of osteogenic genes and related proteins, alkaline phosphatase (ALP) activity, and extracellular matrix mineralization, showing that the SIM-loaded composite coating could upregulate the expression of osteogenic genes and related proteins, promote ALP activity, and enhance extracellular matrix mineralization.
30715842	4	17	theme	contrast to	700:710	arg1	WE43					717:720	contrast to bare WE43	700:720	contrast to bare WE43	700:720	Both the degradation and corrosion rates of the coated substrate were significantly minimized in contrast to bare WE43.
30715842	0	18	from	Coatings	56:63	arg1	Alloy					78:82	Magnesium Alloy	68:82	Magnesium Alloy for Bone Repair	68:98	Electrophoretic Deposition of Bioadaptive Drug Delivery Coatings on Magnesium Alloy for Bone Repair.
30715842	2	19	from	coating	342:348	arg1	substrate					358:366	WE43 substrate	353:366	WE43 substrate	353:366	A gelatin nanospheres/chitosan (GNs/CTS) composite coating on WE43 substrate was fabricated by electrophoretic deposition with simvastatin (SIM) loaded into the GNs.
30715842	3	20	theme	anticorrosion	510:522	arg1	behavior					524:531	 an anticorrosion behavior	506:531	 an anticorrosion behavior of the coated WE43 substrates	506:561	Apart from a sustained drug release over 28 days, an anticorrosion behavior of the coated WE43 substrates was confirmed by electrochemical tests.
30715842	6	21	from	differentiation	848:862	arg1	coatings					900:907	SIM-containing coatings	885:907	SIM-containing coatings	885:907	Additionally, the osteogenic differentiation of MC3T3-E1 cells on SIM-containing coatings was assessed by measuring the expression of osteogenic genes and related proteins, alkaline phosphatase (ALP) activity, and extracellular matrix mineralization, showing that the SIM-loaded composite coating could upregulate the expression of osteogenic genes and related proteins, promote ALP activity, and enhance extracellular matrix mineralization.
30715842	4	22	theme	substrate	658:666	arg1	degradation					612:622	Both the degradation and corrosion rates	603:642	degradation	612:622	Both the degradation and corrosion rates of the coated substrate were significantly minimized in contrast to bare WE43.
30715842	4	22	theme	substrate	658:666	arg1	rates					638:642	Both the degradation and corrosion rates	603:642	rates	638:642	Both the degradation and corrosion rates of the coated substrate were significantly minimized in contrast to bare WE43.
30715842	5	23	theme	samples	759:765	arg1	cytocompatibility					727:743	The cytocompatibility	723:743	The cytocompatibility of the coated samples	723:765	The cytocompatibility of the coated samples was analyzed both quantitatively and qualitatively.
30715842	6	24	theme	related	1172:1178	arg1	proteins					1180:1187	related proteins	1172:1187	related proteins	1172:1187	Additionally, the osteogenic differentiation of MC3T3-E1 cells on SIM-containing coatings was assessed by measuring the expression of osteogenic genes and related proteins, alkaline phosphatase (ALP) activity, and extracellular matrix mineralization, showing that the SIM-loaded composite coating could upregulate the expression of osteogenic genes and related proteins, promote ALP activity, and enhance extracellular matrix mineralization.
30715842	6	25	theme	ALP	1198:1200	arg1	activity					1202:1209	ALP activity	1198:1209	ALP activity	1198:1209	Additionally, the osteogenic differentiation of MC3T3-E1 cells on SIM-containing coatings was assessed by measuring the expression of osteogenic genes and related proteins, alkaline phosphatase (ALP) activity, and extracellular matrix mineralization, showing that the SIM-loaded composite coating could upregulate the expression of osteogenic genes and related proteins, promote ALP activity, and enhance extracellular matrix mineralization.
30715842	7	26	theme	magnesium	1491:1499	arg1	alloys					1501:1506	magnesium alloys	1491:1506	magnesium alloys targeted for orthopedic applications	1491:1543	In summary, the SIM-loaded GNs/CTS composite coatings were able to enhance the corrosion resistance of the WE43 substrate and promote osteogenic activity, thus demonstrating a promising coating system for modifying the surface of magnesium alloys targeted for orthopedic applications.
30715842	4	27	theme	coated	651:656	arg1	substrate					658:666	the coated substrate	647:666	the coated substrate	647:666	Both the degradation and corrosion rates of the coated substrate were significantly minimized in contrast to bare WE43.
30715842	7	28	theme	orthopedic	1521:1530	arg1	applications					1532:1543	orthopedic applications	1521:1543	orthopedic applications	1521:1543	In summary, the SIM-loaded GNs/CTS composite coatings were able to enhance the corrosion resistance of the WE43 substrate and promote osteogenic activity, thus demonstrating a promising coating system for modifying the surface of magnesium alloys targeted for orthopedic applications.
30715842	7	29	theme	WE43	1368:1371	arg1	substrate					1373:1381	the WE43 substrate	1364:1381	the WE43 substrate	1364:1381	In summary, the SIM-loaded GNs/CTS composite coatings were able to enhance the corrosion resistance of the WE43 substrate and promote osteogenic activity, thus demonstrating a promising coating system for modifying the surface of magnesium alloys targeted for orthopedic applications.
30715842	2	30	theme	GNs/CTS	323:329	arg1	coating					342:348	A gelatin nanospheres/chitosan (GNs/CTS) composite coating	291:348	A gelatin nanospheres/chitosan (GNs/CTS) composite coating on WE43 substrate	291:366	A gelatin nanospheres/chitosan (GNs/CTS) composite coating on WE43 substrate was fabricated by electrophoretic deposition with simvastatin (SIM) loaded into the GNs.
30715842	2	31	theme	electrophoretic	386:400	arg1	deposition					402:411	electrophoretic deposition	386:411	electrophoretic deposition with simvastatin (SIM) loaded into the GNs	386:454	A gelatin nanospheres/chitosan (GNs/CTS) composite coating on WE43 substrate was fabricated by electrophoretic deposition with simvastatin (SIM) loaded into the GNs.
30715842	0	32	theme	Electrophoretic	0:14	arg1	Deposition					16:25	Electrophoretic Deposition	0:25	Electrophoretic Deposition of Bioadaptive Drug Delivery	0:54	Electrophoretic Deposition of Bioadaptive Drug Delivery Coatings on Magnesium Alloy for Bone Repair.
30715842	1	33	theme	drug	276:279	arg1	delivery					281:288	drug delivery	276:288	drug delivery	276:288	Biodegradable polymer coatings on magnesium alloys are attractive, as they can provide corrosion resistance as well as additional functions for biomedical applications, e.g., drug delivery.
30715842	1	34	from	coatings	123:130	arg1	alloys					145:150	magnesium alloys	135:150	magnesium alloys	135:150	Biodegradable polymer coatings on magnesium alloys are attractive, as they can provide corrosion resistance as well as additional functions for biomedical applications, e.g., drug delivery.
30715842	1	35	theme	corrosion	188:196	arg1	resistance					198:207	corrosion resistance	188:207	corrosion resistance as well as additional functions for biomedical applications, e.g., drug delivery	188:288	Biodegradable polymer coatings on magnesium alloys are attractive, as they can provide corrosion resistance as well as additional functions for biomedical applications, e.g., drug delivery.
30715842	0	36	theme	Bioadaptive	30:40	arg1	Delivery					47:54	Bioadaptive Drug Delivery	30:54	Bioadaptive Drug Delivery	30:54	Electrophoretic Deposition of Bioadaptive Drug Delivery Coatings on Magnesium Alloy for Bone Repair.
30715842	7	37	theme	promising	1437:1445	arg1	system					1455:1460	a promising coating system	1435:1460	a promising coating system for modifying the surface of magnesium alloys targeted for orthopedic applications	1435:1543	In summary, the SIM-loaded GNs/CTS composite coatings were able to enhance the corrosion resistance of the WE43 substrate and promote osteogenic activity, thus demonstrating a promising coating system for modifying the surface of magnesium alloys targeted for orthopedic applications.
30715842	2	38	theme	nanospheres/chitosan	301:320	arg1	coating					342:348	A gelatin nanospheres/chitosan (GNs/CTS) composite coating	291:348	A gelatin nanospheres/chitosan (GNs/CTS) composite coating on WE43 substrate	291:366	A gelatin nanospheres/chitosan (GNs/CTS) composite coating on WE43 substrate was fabricated by electrophoretic deposition with simvastatin (SIM) loaded into the GNs.
30715842	6	39	theme	composite	1098:1106	arg1	coating					1108:1114	the SIM-loaded composite coating	1083:1114	the SIM-loaded composite coating	1083:1114	Additionally, the osteogenic differentiation of MC3T3-E1 cells on SIM-containing coatings was assessed by measuring the expression of osteogenic genes and related proteins, alkaline phosphatase (ALP) activity, and extracellular matrix mineralization, showing that the SIM-loaded composite coating could upregulate the expression of osteogenic genes and related proteins, promote ALP activity, and enhance extracellular matrix mineralization.
30715842	1	40	theme	polymer	115:121	arg1	coatings					123:130	Biodegradable polymer coatings	101:130	Biodegradable polymer coatings on magnesium alloys	101:150	Biodegradable polymer coatings on magnesium alloys are attractive, as they can provide corrosion resistance as well as additional functions for biomedical applications, e.g., drug delivery.
30715842	4	41	theme	corrosion	628:636	arg1	rates					638:642	Both the degradation and corrosion rates	603:642	rates	638:642	Both the degradation and corrosion rates of the coated substrate were significantly minimized in contrast to bare WE43.
30715842	0	42	theme	Delivery	47:54	arg1	Deposition					16:25	Electrophoretic Deposition	0:25	Electrophoretic Deposition of Bioadaptive Drug Delivery	0:54	Electrophoretic Deposition of Bioadaptive Drug Delivery Coatings on Magnesium Alloy for Bone Repair.
30715842	6	43	theme	phosphatase	1001:1011	arg1	activity					1019:1026	alkaline phosphatase (ALP) activity	992:1026	alkaline phosphatase (ALP) activity	992:1026	Additionally, the osteogenic differentiation of MC3T3-E1 cells on SIM-containing coatings was assessed by measuring the expression of osteogenic genes and related proteins, alkaline phosphatase (ALP) activity, and extracellular matrix mineralization, showing that the SIM-loaded composite coating could upregulate the expression of osteogenic genes and related proteins, promote ALP activity, and enhance extracellular matrix mineralization.
30715842	7	44	theme	coating	1447:1453	arg1	system					1455:1460	a promising coating system	1435:1460	a promising coating system for modifying the surface of magnesium alloys targeted for orthopedic applications	1435:1543	In summary, the SIM-loaded GNs/CTS composite coatings were able to enhance the corrosion resistance of the WE43 substrate and promote osteogenic activity, thus demonstrating a promising coating system for modifying the surface of magnesium alloys targeted for orthopedic applications.
30715842	0	45	theme	Drug	42:45	arg1	Delivery					47:54	Bioadaptive Drug Delivery	30:54	Bioadaptive Drug Delivery	30:54	Electrophoretic Deposition of Bioadaptive Drug Delivery Coatings on Magnesium Alloy for Bone Repair.
30715842	6	46	theme	alkaline	992:999	arg1	ALP					1014:1016	ALP	1014:1016	ALP	1014:1016	Additionally, the osteogenic differentiation of MC3T3-E1 cells on SIM-containing coatings was assessed by measuring the expression of osteogenic genes and related proteins, alkaline phosphatase (ALP) activity, and extracellular matrix mineralization, showing that the SIM-loaded composite coating could upregulate the expression of osteogenic genes and related proteins, promote ALP activity, and enhance extracellular matrix mineralization.
30715842	6	46	theme	alkaline	992:999	arg1	phosphatase					1001:1011	alkaline phosphatase	992:1011	alkaline phosphatase (ALP) activity	992:1026	Additionally, the osteogenic differentiation of MC3T3-E1 cells on SIM-containing coatings was assessed by measuring the expression of osteogenic genes and related proteins, alkaline phosphatase (ALP) activity, and extracellular matrix mineralization, showing that the SIM-loaded composite coating could upregulate the expression of osteogenic genes and related proteins, promote ALP activity, and enhance extracellular matrix mineralization.
30715842	6	47	theme	SIM-containing	885:898	arg1	coatings					900:907	SIM-containing coatings	885:907	SIM-containing coatings	885:907	Additionally, the osteogenic differentiation of MC3T3-E1 cells on SIM-containing coatings was assessed by measuring the expression of osteogenic genes and related proteins, alkaline phosphatase (ALP) activity, and extracellular matrix mineralization, showing that the SIM-loaded composite coating could upregulate the expression of osteogenic genes and related proteins, promote ALP activity, and enhance extracellular matrix mineralization.
30715842	2	48	with	deposition	402:411	arg1	SIM					431:433	SIM	431:433	SIM	431:433	A gelatin nanospheres/chitosan (GNs/CTS) composite coating on WE43 substrate was fabricated by electrophoretic deposition with simvastatin (SIM) loaded into the GNs.
30715842	2	48	with	deposition	402:411	arg1	simvastatin					418:428	simvastatin	418:428	simvastatin (SIM) loaded into the GNs	418:454	A gelatin nanospheres/chitosan (GNs/CTS) composite coating on WE43 substrate was fabricated by electrophoretic deposition with simvastatin (SIM) loaded into the GNs.
30715842	6	49	theme	proteins	982:989	arg1	mineralization					1054:1067	extracellular matrix mineralization	1033:1067	extracellular matrix mineralization	1033:1067	Additionally, the osteogenic differentiation of MC3T3-E1 cells on SIM-containing coatings was assessed by measuring the expression of osteogenic genes and related proteins, alkaline phosphatase (ALP) activity, and extracellular matrix mineralization, showing that the SIM-loaded composite coating could upregulate the expression of osteogenic genes and related proteins, promote ALP activity, and enhance extracellular matrix mineralization.
30715842	6	49	theme	proteins	982:989	arg1	activity					1019:1026	alkaline phosphatase (ALP) activity	992:1026	alkaline phosphatase (ALP) activity	992:1026	Additionally, the osteogenic differentiation of MC3T3-E1 cells on SIM-containing coatings was assessed by measuring the expression of osteogenic genes and related proteins, alkaline phosphatase (ALP) activity, and extracellular matrix mineralization, showing that the SIM-loaded composite coating could upregulate the expression of osteogenic genes and related proteins, promote ALP activity, and enhance extracellular matrix mineralization.
30715842	6	49	theme	proteins	982:989	arg1	expression					939:948	the expression	935:948	the expression of osteogenic genes and related proteins	935:989	Additionally, the osteogenic differentiation of MC3T3-E1 cells on SIM-containing coatings was assessed by measuring the expression of osteogenic genes and related proteins, alkaline phosphatase (ALP) activity, and extracellular matrix mineralization, showing that the SIM-loaded composite coating could upregulate the expression of osteogenic genes and related proteins, promote ALP activity, and enhance extracellular matrix mineralization.
30715842	3	50	theme	sustained	470:478	arg1	release					485:491	a sustained drug release	468:491	a sustained drug release over 28 days	468:504	Apart from a sustained drug release over 28 days, an anticorrosion behavior of the coated WE43 substrates was confirmed by electrochemical tests.
30715842	3	51	theme	electrochemical	580:594	arg1	tests					596:600	electrochemical tests	580:600	electrochemical tests	580:600	Apart from a sustained drug release over 28 days, an anticorrosion behavior of the coated WE43 substrates was confirmed by electrochemical tests.
30715842	7	52	theme	osteogenic	1395:1404	arg1	activity					1406:1413	osteogenic activity	1395:1413	osteogenic activity	1395:1413	In summary, the SIM-loaded GNs/CTS composite coatings were able to enhance the corrosion resistance of the WE43 substrate and promote osteogenic activity, thus demonstrating a promising coating system for modifying the surface of magnesium alloys targeted for orthopedic applications.
30715842	6	53	theme	proteins	1180:1187	arg1	expression					1137:1146	the expression	1133:1146	the expression of osteogenic genes and related proteins	1133:1187	Additionally, the osteogenic differentiation of MC3T3-E1 cells on SIM-containing coatings was assessed by measuring the expression of osteogenic genes and related proteins, alkaline phosphatase (ALP) activity, and extracellular matrix mineralization, showing that the SIM-loaded composite coating could upregulate the expression of osteogenic genes and related proteins, promote ALP activity, and enhance extracellular matrix mineralization.
30715842	6	54	theme	related	974:980	arg1	proteins					982:989	related proteins	974:989	related proteins	974:989	Additionally, the osteogenic differentiation of MC3T3-E1 cells on SIM-containing coatings was assessed by measuring the expression of osteogenic genes and related proteins, alkaline phosphatase (ALP) activity, and extracellular matrix mineralization, showing that the SIM-loaded composite coating could upregulate the expression of osteogenic genes and related proteins, promote ALP activity, and enhance extracellular matrix mineralization.
30715842	6	55	theme	cells	876:880	arg1	differentiation					848:862	the osteogenic differentiation	833:862	the osteogenic differentiation of MC3T3-E1 cells on SIM-containing coatings	833:907	Additionally, the osteogenic differentiation of MC3T3-E1 cells on SIM-containing coatings was assessed by measuring the expression of osteogenic genes and related proteins, alkaline phosphatase (ALP) activity, and extracellular matrix mineralization, showing that the SIM-loaded composite coating could upregulate the expression of osteogenic genes and related proteins, promote ALP activity, and enhance extracellular matrix mineralization.
30715842	7	56	from	summary	1264:1270	arg1	able					1320:1323	able	1320:1323	able	1320:1323	In summary, the SIM-loaded GNs/CTS composite coatings were able to enhance the corrosion resistance of the WE43 substrate and promote osteogenic activity, thus demonstrating a promising coating system for modifying the surface of magnesium alloys targeted for orthopedic applications.
30715842	7	56	from	summary	1264:1270	arg1	coatings					1306:1313	the SIM-loaded GNs/CTS composite coatings	1273:1313	the SIM-loaded GNs/CTS composite coatings	1273:1313	In summary, the SIM-loaded GNs/CTS composite coatings were able to enhance the corrosion resistance of the WE43 substrate and promote osteogenic activity, thus demonstrating a promising coating system for modifying the surface of magnesium alloys targeted for orthopedic applications.
30715842	1	57	theme	magnesium	135:143	arg1	alloys					145:150	magnesium alloys	135:150	magnesium alloys	135:150	Biodegradable polymer coatings on magnesium alloys are attractive, as they can provide corrosion resistance as well as additional functions for biomedical applications, e.g., drug delivery.
30715842	1	58	theme	Biodegradable	101:113	arg1	coatings					123:130	Biodegradable polymer coatings	101:130	Biodegradable polymer coatings on magnesium alloys	101:150	Biodegradable polymer coatings on magnesium alloys are attractive, as they can provide corrosion resistance as well as additional functions for biomedical applications, e.g., drug delivery.
30715842	1	59	theme	additional	220:229	arg1	functions					231:239	additional functions	220:239	additional functions	220:239	Biodegradable polymer coatings on magnesium alloys are attractive, as they can provide corrosion resistance as well as additional functions for biomedical applications, e.g., drug delivery.
30715842	6	60	theme	MC3T3-E1	867:874	arg1	cells					876:880	MC3T3-E1 cells	867:880	MC3T3-E1 cells	867:880	Additionally, the osteogenic differentiation of MC3T3-E1 cells on SIM-containing coatings was assessed by measuring the expression of osteogenic genes and related proteins, alkaline phosphatase (ALP) activity, and extracellular matrix mineralization, showing that the SIM-loaded composite coating could upregulate the expression of osteogenic genes and related proteins, promote ALP activity, and enhance extracellular matrix mineralization.
30715842	7	61	theme	alloys	1501:1506	arg1	surface					1480:1486	the surface	1476:1486	the surface of magnesium alloys targeted for orthopedic applications	1476:1543	In summary, the SIM-loaded GNs/CTS composite coatings were able to enhance the corrosion resistance of the WE43 substrate and promote osteogenic activity, thus demonstrating a promising coating system for modifying the surface of magnesium alloys targeted for orthopedic applications.
30715842	3	62	theme	drug	480:483	arg1	release					485:491	a sustained drug release	468:491	a sustained drug release over 28 days	468:504	Apart from a sustained drug release over 28 days, an anticorrosion behavior of the coated WE43 substrates was confirmed by electrochemical tests.
30715842	7	63	theme	substrate	1373:1381	arg1	resistance					1350:1359	the corrosion resistance	1336:1359	the corrosion resistance of the WE43 substrate	1336:1381	In summary, the SIM-loaded GNs/CTS composite coatings were able to enhance the corrosion resistance of the WE43 substrate and promote osteogenic activity, thus demonstrating a promising coating system for modifying the surface of magnesium alloys targeted for orthopedic applications.
30715842	2	64	theme	composite	332:340	arg1	coating					342:348	A gelatin nanospheres/chitosan (GNs/CTS) composite coating	291:348	A gelatin nanospheres/chitosan (GNs/CTS) composite coating on WE43 substrate	291:366	A gelatin nanospheres/chitosan (GNs/CTS) composite coating on WE43 substrate was fabricated by electrophoretic deposition with simvastatin (SIM) loaded into the GNs.
30715842	7	65	theme	SIM-loaded	1277:1286	arg1	able					1320:1323	able	1320:1323	able	1320:1323	In summary, the SIM-loaded GNs/CTS composite coatings were able to enhance the corrosion resistance of the WE43 substrate and promote osteogenic activity, thus demonstrating a promising coating system for modifying the surface of magnesium alloys targeted for orthopedic applications.
30715842	7	65	theme	SIM-loaded	1277:1286	arg1	coatings					1306:1313	the SIM-loaded GNs/CTS composite coatings	1273:1313	the SIM-loaded GNs/CTS composite coatings	1273:1313	In summary, the SIM-loaded GNs/CTS composite coatings were able to enhance the corrosion resistance of the WE43 substrate and promote osteogenic activity, thus demonstrating a promising coating system for modifying the surface of magnesium alloys targeted for orthopedic applications.
30715842	5	66	theme	coated	752:757	arg1	samples					759:765	the coated samples	748:765	the coated samples	748:765	The cytocompatibility of the coated samples was analyzed both quantitatively and qualitatively.
30715842	6	67	theme	genes	1162:1166	arg1	expression					1137:1146	the expression	1133:1146	the expression of osteogenic genes and related proteins	1133:1187	Additionally, the osteogenic differentiation of MC3T3-E1 cells on SIM-containing coatings was assessed by measuring the expression of osteogenic genes and related proteins, alkaline phosphatase (ALP) activity, and extracellular matrix mineralization, showing that the SIM-loaded composite coating could upregulate the expression of osteogenic genes and related proteins, promote ALP activity, and enhance extracellular matrix mineralization.
30715842	7	68	theme	GNs/CTS	1288:1294	arg1	able					1320:1323	able	1320:1323	able	1320:1323	In summary, the SIM-loaded GNs/CTS composite coatings were able to enhance the corrosion resistance of the WE43 substrate and promote osteogenic activity, thus demonstrating a promising coating system for modifying the surface of magnesium alloys targeted for orthopedic applications.
30715842	7	68	theme	GNs/CTS	1288:1294	arg1	coatings					1306:1313	the SIM-loaded GNs/CTS composite coatings	1273:1313	the SIM-loaded GNs/CTS composite coatings	1273:1313	In summary, the SIM-loaded GNs/CTS composite coatings were able to enhance the corrosion resistance of the WE43 substrate and promote osteogenic activity, thus demonstrating a promising coating system for modifying the surface of magnesium alloys targeted for orthopedic applications.
30715842	7	69	from	able	1320:1323	arg1	summary					1264:1270	summary	1264:1270	summary	1264:1270	In summary, the SIM-loaded GNs/CTS composite coatings were able to enhance the corrosion resistance of the WE43 substrate and promote osteogenic activity, thus demonstrating a promising coating system for modifying the surface of magnesium alloys targeted for orthopedic applications.
30715842	0	70	theme	Magnesium	68:76	arg1	Alloy					78:82	Magnesium Alloy	68:82	Magnesium Alloy for Bone Repair	68:98	Electrophoretic Deposition of Bioadaptive Drug Delivery Coatings on Magnesium Alloy for Bone Repair.
30715842	6	71	theme	matrix	1047:1052	arg1	mineralization					1054:1067	extracellular matrix mineralization	1033:1067	extracellular matrix mineralization	1033:1067	Additionally, the osteogenic differentiation of MC3T3-E1 cells on SIM-containing coatings was assessed by measuring the expression of osteogenic genes and related proteins, alkaline phosphatase (ALP) activity, and extracellular matrix mineralization, showing that the SIM-loaded composite coating could upregulate the expression of osteogenic genes and related proteins, promote ALP activity, and enhance extracellular matrix mineralization.
30715842	3	72	theme	 an	506:508	arg1	behavior					524:531	 an anticorrosion behavior	506:531	 an anticorrosion behavior of the coated WE43 substrates	506:561	Apart from a sustained drug release over 28 days, an anticorrosion behavior of the coated WE43 substrates was confirmed by electrochemical tests.
30715842	7	73	theme	corrosion	1340:1348	arg1	resistance					1350:1359	the corrosion resistance	1336:1359	the corrosion resistance of the WE43 substrate	1336:1381	In summary, the SIM-loaded GNs/CTS composite coatings were able to enhance the corrosion resistance of the WE43 substrate and promote osteogenic activity, thus demonstrating a promising coating system for modifying the surface of magnesium alloys targeted for orthopedic applications.
30715842	1	74	theme	biomedical	245:254	arg1	applications					256:267	biomedical applications	245:267	biomedical applications	245:267	Biodegradable polymer coatings on magnesium alloys are attractive, as they can provide corrosion resistance as well as additional functions for biomedical applications, e.g., drug delivery.
30381626	11	0	theme	Increased	1654:1662	arg1	permeability					1668:1679	Increased gut permeability	1654:1679	Increased gut permeability in SP+FOS	1654:1689	Increased gut permeability in SP+FOS could be evoked by the disruption of the mucus layer due to stasis of the very acidic luminal contents.
30381626	4	1	from	responses	587:595	arg1	experiment					611:620	experiment 1	611:622	experiment 1	611:622	We assessed microbial fermentation, gut permeability, and inflammatory responses in the cecum (experiment 1), and mucus layer in the cecum, intestinal transit time and microbiota composition (experiment 2).
30381626	4	1	from	responses	587:595	arg1	cecum					604:608	the cecum	600:608	the cecum (experiment 1)	600:623	We assessed microbial fermentation, gut permeability, and inflammatory responses in the cecum (experiment 1), and mucus layer in the cecum, intestinal transit time and microbiota composition (experiment 2).
30381626	9	2	theme	microbiota	1456:1465	arg1	richness					1438:1445	Lower species richness	1424:1445	Lower species richness of cecal microbiota	1424:1465	Lower species richness of cecal microbiota was manifest solely in SP+FOS (experiment 2).
30381626	6	3	theme	cytokine	1069:1076	arg1	genes					1078:1082	the inflammatory cytokine genes	1052:1082	the inflammatory cytokine genes in the cecal mucosa	1052:1102	Gut permeability estimated by urinary chromium-EDTA excretion, bacterial translocation into mesenteric lymph nodes, myeloperoxidase activity, and expressions of the inflammatory cytokine genes in the cecal mucosa were greater in SP+FOS than in SP, but these alterations were not observed between NP and NP+FOS (experiment 1).
30381626	11	4	theme	luminal	1777:1783	arg1	contents					1785:1792	the very acidic luminal contents	1761:1792	the very acidic luminal contents	1761:1792	Increased gut permeability in SP+FOS could be evoked by the disruption of the mucus layer due to stasis of the very acidic luminal contents.
30381626	11	5	theme	acidic	1770:1775	arg1	contents					1785:1792	the very acidic luminal contents	1761:1792	the very acidic luminal contents	1761:1792	Increased gut permeability in SP+FOS could be evoked by the disruption of the mucus layer due to stasis of the very acidic luminal contents.
30381626	7	6	theme	epithelial	1270:1279	arg1	surface					1281:1287	the epithelial surface	1266:1287	the epithelial surface	1266:1287	FOS supplementation destroyed the mucus layer on the epithelial surface in SP, but not in NP.
30381626	4	7	from	permeability	556:567	arg1	experiment					611:620	experiment 1	611:622	experiment 1	611:622	We assessed microbial fermentation, gut permeability, and inflammatory responses in the cecum (experiment 1), and mucus layer in the cecum, intestinal transit time and microbiota composition (experiment 2).
30381626	4	7	from	permeability	556:567	arg1	cecum					604:608	the cecum	600:608	the cecum (experiment 1)	600:623	We assessed microbial fermentation, gut permeability, and inflammatory responses in the cecum (experiment 1), and mucus layer in the cecum, intestinal transit time and microbiota composition (experiment 2).
30381626	1	8	theme	fructo-oligosaccharides	189:211	arg1	effects					178:184	The effects	174:184	The effects of fructo-oligosaccharides (FOS) on gut-barrier function	174:241	The effects of fructo-oligosaccharides (FOS) on gut-barrier function are still controversial in human and animal studies.
30381626	1	8	theme	fructo-oligosaccharides	189:211	arg1	controversial					253:265	controversial	253:265	controversial	253:265	The effects of fructo-oligosaccharides (FOS) on gut-barrier function are still controversial in human and animal studies.
30381626	7	9	theme	mucus	1251:1255	arg1	layer					1257:1261	the mucus layer	1247:1261	the mucus layer on the epithelial surface	1247:1287	FOS supplementation destroyed the mucus layer on the epithelial surface in SP, but not in NP.
30381626	4	10	theme	microbial	528:536	arg1	fermentation					538:549	microbial fermentation	528:549	microbial fermentation	528:549	We assessed microbial fermentation, gut permeability, and inflammatory responses in the cecum (experiment 1), and mucus layer in the cecum, intestinal transit time and microbiota composition (experiment 2).
30381626	5	11	from	products	875:882	arg1	NP					887:888	NP	887:888	NP	887:888	FOS supplementation induced a very acidic fermentation due to the accumulation of lactate and succinate in SP, while short-chain fatty acids were major products in NP.
30381626	11	12	theme	layer	1738:1742	arg1	disruption					1714:1723	the disruption	1710:1723	the disruption of the mucus layer due to stasis of the very acidic luminal contents	1710:1792	Increased gut permeability in SP+FOS could be evoked by the disruption of the mucus layer due to stasis of the very acidic luminal contents.
30381626	7	13	theme	FOS	1217:1219	arg1	supplementation					1221:1235	FOS supplementation	1217:1235	FOS supplementation	1217:1235	FOS supplementation destroyed the mucus layer on the epithelial surface in SP, but not in NP.
30381626	10	14	theme	inflammatory	1578:1589	arg1	responses					1591:1599	inflammatory responses	1578:1599	inflammatory responses in the cecal mucosa	1578:1619	These factors suggest that impact of FOS on gut permeability and inflammatory responses in the cecal mucosa quite differs between SP and NP.
30381626	10	15	from	impact	1540:1545	arg1	permeability					1561:1572	gut permeability	1557:1572	gut permeability	1557:1572	These factors suggest that impact of FOS on gut permeability and inflammatory responses in the cecal mucosa quite differs between SP and NP.
30381626	10	15	from	impact	1540:1545	arg1	responses					1591:1599	inflammatory responses	1578:1599	inflammatory responses in the cecal mucosa	1578:1619	These factors suggest that impact of FOS on gut permeability and inflammatory responses in the cecal mucosa quite differs between SP and NP.
30381626	6	16	theme	cecal	1091:1095	arg1	mucosa					1097:1102	the cecal mucosa	1087:1102	the cecal mucosa	1087:1102	Gut permeability estimated by urinary chromium-EDTA excretion, bacterial translocation into mesenteric lymph nodes, myeloperoxidase activity, and expressions of the inflammatory cytokine genes in the cecal mucosa were greater in SP+FOS than in SP, but these alterations were not observed between NP and NP+FOS (experiment 1).
30381626	6	17	from	mucosa	1097:1102	arg1	activity					1023:1030	myeloperoxidase activity	1007:1030	myeloperoxidase activity	1007:1030	Gut permeability estimated by urinary chromium-EDTA excretion, bacterial translocation into mesenteric lymph nodes, myeloperoxidase activity, and expressions of the inflammatory cytokine genes in the cecal mucosa were greater in SP+FOS than in SP, but these alterations were not observed between NP and NP+FOS (experiment 1).
30381626	6	17	from	mucosa	1097:1102	arg1	expressions					1037:1047	expressions	1037:1047	expressions of the inflammatory cytokine genes in the cecal mucosa	1037:1102	Gut permeability estimated by urinary chromium-EDTA excretion, bacterial translocation into mesenteric lymph nodes, myeloperoxidase activity, and expressions of the inflammatory cytokine genes in the cecal mucosa were greater in SP+FOS than in SP, but these alterations were not observed between NP and NP+FOS (experiment 1).
30381626	6	17	from	mucosa	1097:1102	arg1	excretion					943:951	urinary chromium-EDTA excretion	921:951	urinary chromium-EDTA excretion	921:951	Gut permeability estimated by urinary chromium-EDTA excretion, bacterial translocation into mesenteric lymph nodes, myeloperoxidase activity, and expressions of the inflammatory cytokine genes in the cecal mucosa were greater in SP+FOS than in SP, but these alterations were not observed between NP and NP+FOS (experiment 1).
30381626	6	17	from	mucosa	1097:1102	arg1	translocation					964:976	bacterial translocation	954:976	bacterial translocation into mesenteric lymph nodes	954:1004	Gut permeability estimated by urinary chromium-EDTA excretion, bacterial translocation into mesenteric lymph nodes, myeloperoxidase activity, and expressions of the inflammatory cytokine genes in the cecal mucosa were greater in SP+FOS than in SP, but these alterations were not observed between NP and NP+FOS (experiment 1).
30381626	10	18	theme	FOS	1550:1552	arg1	impact					1540:1545	impact	1540:1545	impact of FOS on gut permeability and inflammatory responses in the cecal mucosa	1540:1619	These factors suggest that impact of FOS on gut permeability and inflammatory responses in the cecal mucosa quite differs between SP and NP.
30381626	8	19	theme	Intestinal	1311:1320	arg1	time					1330:1333	Intestinal transit time	1311:1333	Intestinal transit time	1311:1333	Intestinal transit time was 3-fold longer in SP+FOS than in SP, but this was not the case between NP and NP+FOS.
30381626	0	20	theme	Inflammatory	62:73	arg1	Responses					75:83	Inflammatory Responses	62:83	Inflammatory Responses in the Cecal Mucosa	62:103	The Impact of Fructo-Oligosaccharides on Gut Permeability and Inflammatory Responses in the Cecal Mucosa Quite Differs between Rats Fed Semi-Purified and Non-Purified Diets.
30381626	10	21	theme	cecal	1608:1612	arg1	mucosa					1614:1619	the cecal mucosa	1604:1619	the cecal mucosa	1604:1619	These factors suggest that impact of FOS on gut permeability and inflammatory responses in the cecal mucosa quite differs between SP and NP.
30381626	5	22	theme	acidic	758:763	arg1	fermentation					765:776	a very acidic fermentation	751:776	a very acidic fermentation due to the accumulation of lactate and succinate in SP	751:831	FOS supplementation induced a very acidic fermentation due to the accumulation of lactate and succinate in SP, while short-chain fatty acids were major products in NP.
30381626	6	23	from	genes	1078:1082	arg1	mucosa					1097:1102	the cecal mucosa	1087:1102	the cecal mucosa	1087:1102	Gut permeability estimated by urinary chromium-EDTA excretion, bacterial translocation into mesenteric lymph nodes, myeloperoxidase activity, and expressions of the inflammatory cytokine genes in the cecal mucosa were greater in SP+FOS than in SP, but these alterations were not observed between NP and NP+FOS (experiment 1).
30381626	5	24	theme	due	778:780	arg1	fermentation					765:776	a very acidic fermentation	751:776	a very acidic fermentation due to the accumulation of lactate and succinate in SP	751:831	FOS supplementation induced a very acidic fermentation due to the accumulation of lactate and succinate in SP, while short-chain fatty acids were major products in NP.
30381626	0	25	theme	Cecal	92:96	arg1	Mucosa					98:103	the Cecal Mucosa	88:103	the Cecal Mucosa	88:103	The Impact of Fructo-Oligosaccharides on Gut Permeability and Inflammatory Responses in the Cecal Mucosa Quite Differs between Rats Fed Semi-Purified and Non-Purified Diets.
30381626	10	26	from	responses	1591:1599	arg1	mucosa					1614:1619	the cecal mucosa	1604:1619	the cecal mucosa	1604:1619	These factors suggest that impact of FOS on gut permeability and inflammatory responses in the cecal mucosa quite differs between SP and NP.
30381626	0	27	from	Permeability	45:56	arg1	Mucosa					98:103	the Cecal Mucosa	88:103	the Cecal Mucosa	88:103	The Impact of Fructo-Oligosaccharides on Gut Permeability and Inflammatory Responses in the Cecal Mucosa Quite Differs between Rats Fed Semi-Purified and Non-Purified Diets.
30381626	11	28	from	permeability	1668:1679	arg1	SP+FOS					1684:1689	SP+FOS	1684:1689	SP+FOS	1684:1689	Increased gut permeability in SP+FOS could be evoked by the disruption of the mucus layer due to stasis of the very acidic luminal contents.
30381626	5	29	from	accumulation	789:800	arg1	SP					830:831	SP	830:831	SP	830:831	FOS supplementation induced a very acidic fermentation due to the accumulation of lactate and succinate in SP, while short-chain fatty acids were major products in NP.
30381626	5	30	theme	short-chain	840:850	arg1	acids					858:862	short-chain fatty acids	840:862	short-chain fatty acids	840:862	FOS supplementation induced a very acidic fermentation due to the accumulation of lactate and succinate in SP, while short-chain fatty acids were major products in NP.
30381626	6	31	theme	urinary	921:927	arg1	excretion					943:951	urinary chromium-EDTA excretion	921:951	urinary chromium-EDTA excretion	921:951	Gut permeability estimated by urinary chromium-EDTA excretion, bacterial translocation into mesenteric lymph nodes, myeloperoxidase activity, and expressions of the inflammatory cytokine genes in the cecal mucosa were greater in SP+FOS than in SP, but these alterations were not observed between NP and NP+FOS (experiment 1).
30381626	4	32	theme	mucus	630:634	arg1	layer					636:640	mucus layer	630:640	mucus layer	630:640	We assessed microbial fermentation, gut permeability, and inflammatory responses in the cecum (experiment 1), and mucus layer in the cecum, intestinal transit time and microbiota composition (experiment 2).
30381626	6	33	from	activity	1023:1030	arg1	mucosa					1097:1102	the cecal mucosa	1087:1102	the cecal mucosa	1087:1102	Gut permeability estimated by urinary chromium-EDTA excretion, bacterial translocation into mesenteric lymph nodes, myeloperoxidase activity, and expressions of the inflammatory cytokine genes in the cecal mucosa were greater in SP+FOS than in SP, but these alterations were not observed between NP and NP+FOS (experiment 1).
30381626	4	34	theme	microbiota	684:693	arg1	composition					695:705	microbiota composition	684:705	microbiota composition	684:705	We assessed microbial fermentation, gut permeability, and inflammatory responses in the cecum (experiment 1), and mucus layer in the cecum, intestinal transit time and microbiota composition (experiment 2).
30381626	6	35	theme	Gut	891:893	arg1	permeability					895:906	Gut permeability	891:906	Gut permeability estimated by urinary chromium-EDTA excretion, bacterial translocation into mesenteric lymph nodes, myeloperoxidase activity, and expressions of the inflammatory cytokine genes in the cecal mucosa	891:1102	Gut permeability estimated by urinary chromium-EDTA excretion, bacterial translocation into mesenteric lymph nodes, myeloperoxidase activity, and expressions of the inflammatory cytokine genes in the cecal mucosa were greater in SP+FOS than in SP, but these alterations were not observed between NP and NP+FOS (experiment 1).
30381626	4	36	theme	intestinal	656:665	arg1	time					675:678	intestinal transit time	656:678	intestinal transit time	656:678	We assessed microbial fermentation, gut permeability, and inflammatory responses in the cecum (experiment 1), and mucus layer in the cecum, intestinal transit time and microbiota composition (experiment 2).
30381626	1	37	theme	gut-barrier	222:232	arg1	function					234:241	gut-barrier function	222:241	gut-barrier function	222:241	The effects of fructo-oligosaccharides (FOS) on gut-barrier function are still controversial in human and animal studies.
30381626	9	38	theme	Lower	1424:1428	arg1	richness					1438:1445	Lower species richness	1424:1445	Lower species richness of cecal microbiota	1424:1465	Lower species richness of cecal microbiota was manifest solely in SP+FOS (experiment 2).
30381626	6	39	theme	lymph	994:998	arg1	nodes					1000:1004	mesenteric lymph nodes	983:1004	mesenteric lymph nodes	983:1004	Gut permeability estimated by urinary chromium-EDTA excretion, bacterial translocation into mesenteric lymph nodes, myeloperoxidase activity, and expressions of the inflammatory cytokine genes in the cecal mucosa were greater in SP+FOS than in SP, but these alterations were not observed between NP and NP+FOS (experiment 1).
30381626	11	40	theme	gut	1664:1666	arg1	permeability					1668:1679	Increased gut permeability	1654:1679	Increased gut permeability in SP+FOS	1654:1689	Increased gut permeability in SP+FOS could be evoked by the disruption of the mucus layer due to stasis of the very acidic luminal contents.
30381626	5	41	theme	major	869:873	arg1	products					875:882	major products	869:882	major products in NP	869:888	FOS supplementation induced a very acidic fermentation due to the accumulation of lactate and succinate in SP, while short-chain fatty acids were major products in NP.
30381626	9	42	theme	cecal	1450:1454	arg1	microbiota					1456:1465	cecal microbiota	1450:1465	cecal microbiota	1450:1465	Lower species richness of cecal microbiota was manifest solely in SP+FOS (experiment 2).
30381626	2	43	from	factor	329:334	arg1	studies					366:372	animal studies	359:372	animal studies	359:372	Diet conditions would be a major factor for the controversy in animal studies.
30381626	6	44	theme	bacterial	954:962	arg1	translocation					964:976	bacterial translocation	954:976	bacterial translocation into mesenteric lymph nodes	954:1004	Gut permeability estimated by urinary chromium-EDTA excretion, bacterial translocation into mesenteric lymph nodes, myeloperoxidase activity, and expressions of the inflammatory cytokine genes in the cecal mucosa were greater in SP+FOS than in SP, but these alterations were not observed between NP and NP+FOS (experiment 1).
30381626	2	45	theme	Diet	296:299	arg1	factor					329:334	a major factor	321:334	a major factor for the controversy in animal studies	321:372	Diet conditions would be a major factor for the controversy in animal studies.
30381626	2	45	theme	Diet	296:299	arg1	conditions					301:310	Diet conditions	296:310	Diet conditions	296:310	Diet conditions would be a major factor for the controversy in animal studies.
30381626	6	46	theme	inflammatory	1056:1067	arg1	genes					1078:1082	the inflammatory cytokine genes	1052:1082	the inflammatory cytokine genes in the cecal mucosa	1052:1102	Gut permeability estimated by urinary chromium-EDTA excretion, bacterial translocation into mesenteric lymph nodes, myeloperoxidase activity, and expressions of the inflammatory cytokine genes in the cecal mucosa were greater in SP+FOS than in SP, but these alterations were not observed between NP and NP+FOS (experiment 1).
30381626	6	47	from	excretion	943:951	arg1	mucosa					1097:1102	the cecal mucosa	1087:1102	the cecal mucosa	1087:1102	Gut permeability estimated by urinary chromium-EDTA excretion, bacterial translocation into mesenteric lymph nodes, myeloperoxidase activity, and expressions of the inflammatory cytokine genes in the cecal mucosa were greater in SP+FOS than in SP, but these alterations were not observed between NP and NP+FOS (experiment 1).
30381626	5	48	theme	FOS	723:725	arg1	supplementation					727:741	FOS supplementation	723:741	FOS supplementation	723:741	FOS supplementation induced a very acidic fermentation due to the accumulation of lactate and succinate in SP, while short-chain fatty acids were major products in NP.
30381626	4	49	theme	gut	552:554	arg1	permeability					556:567	gut permeability	552:567	gut permeability	552:567	We assessed microbial fermentation, gut permeability, and inflammatory responses in the cecum (experiment 1), and mucus layer in the cecum, intestinal transit time and microbiota composition (experiment 2).
30381626	1	50	from	animal	280:285	arg1	effects					178:184	The effects	174:184	The effects of fructo-oligosaccharides (FOS) on gut-barrier function	174:241	The effects of fructo-oligosaccharides (FOS) on gut-barrier function are still controversial in human and animal studies.
30381626	1	50	from	animal	280:285	arg1	controversial					253:265	controversial	253:265	controversial	253:265	The effects of fructo-oligosaccharides (FOS) on gut-barrier function are still controversial in human and animal studies.
30381626	1	51	from	human	270:274	arg1	effects					178:184	The effects	174:184	The effects of fructo-oligosaccharides (FOS) on gut-barrier function	174:241	The effects of fructo-oligosaccharides (FOS) on gut-barrier function are still controversial in human and animal studies.
30381626	1	51	from	human	270:274	arg1	controversial					253:265	controversial	253:265	controversial	253:265	The effects of fructo-oligosaccharides (FOS) on gut-barrier function are still controversial in human and animal studies.
30381626	10	52	theme	gut	1557:1559	arg1	permeability					1561:1572	gut permeability	1557:1572	gut permeability	1557:1572	These factors suggest that impact of FOS on gut permeability and inflammatory responses in the cecal mucosa quite differs between SP and NP.
30381626	4	53	from	fermentation	538:549	arg1	experiment					611:620	experiment 1	611:622	experiment 1	611:622	We assessed microbial fermentation, gut permeability, and inflammatory responses in the cecum (experiment 1), and mucus layer in the cecum, intestinal transit time and microbiota composition (experiment 2).
30381626	4	53	from	fermentation	538:549	arg1	cecum					604:608	the cecum	600:608	the cecum (experiment 1)	600:623	We assessed microbial fermentation, gut permeability, and inflammatory responses in the cecum (experiment 1), and mucus layer in the cecum, intestinal transit time and microbiota composition (experiment 2).
30381626	0	54	theme	Semi-Purified	136:148	arg1	Diets					167:171	Semi-Purified and Non-Purified Diets	136:171	Semi-Purified and Non-Purified Diets	136:171	The Impact of Fructo-Oligosaccharides on Gut Permeability and Inflammatory Responses in the Cecal Mucosa Quite Differs between Rats Fed Semi-Purified and Non-Purified Diets.
30381626	11	55	theme	mucus	1732:1736	arg1	layer					1738:1742	the mucus layer	1728:1742	the mucus layer due to stasis of the very acidic luminal contents	1728:1792	Increased gut permeability in SP+FOS could be evoked by the disruption of the mucus layer due to stasis of the very acidic luminal contents.
30381626	0	56	from	Responses	75:83	arg1	Mucosa					98:103	the Cecal Mucosa	88:103	the Cecal Mucosa	88:103	The Impact of Fructo-Oligosaccharides on Gut Permeability and Inflammatory Responses in the Cecal Mucosa Quite Differs between Rats Fed Semi-Purified and Non-Purified Diets.
30381626	0	57	theme	Non-Purified	154:165	arg1	Diets					167:171	Semi-Purified and Non-Purified Diets	136:171	Semi-Purified and Non-Purified Diets	136:171	The Impact of Fructo-Oligosaccharides on Gut Permeability and Inflammatory Responses in the Cecal Mucosa Quite Differs between Rats Fed Semi-Purified and Non-Purified Diets.
30381626	1	58	dep	human	270:274	arg1	studies					287:293	studies	287:293	studies	287:293	The effects of fructo-oligosaccharides (FOS) on gut-barrier function are still controversial in human and animal studies.
30381626	7	59	from	layer	1257:1261	arg1	surface					1281:1287	the epithelial surface	1266:1287	the epithelial surface	1266:1287	FOS supplementation destroyed the mucus layer on the epithelial surface in SP, but not in NP.
30381626	8	60	theme	transit	1322:1328	arg1	time					1330:1333	Intestinal transit time	1311:1333	Intestinal transit time	1311:1333	Intestinal transit time was 3-fold longer in SP+FOS than in SP, but this was not the case between NP and NP+FOS.
30381626	11	61	theme	due	1744:1746	arg1	layer					1738:1742	the mucus layer	1728:1742	the mucus layer due to stasis of the very acidic luminal contents	1728:1792	Increased gut permeability in SP+FOS could be evoked by the disruption of the mucus layer due to stasis of the very acidic luminal contents.
30381626	4	62	dep	cecum	649:653	arg1	the					645:647	the	645:647	the	645:647	We assessed microbial fermentation, gut permeability, and inflammatory responses in the cecum (experiment 1), and mucus layer in the cecum, intestinal transit time and microbiota composition (experiment 2).
30381626	6	63	theme	genes	1078:1082	arg1	activity					1023:1030	myeloperoxidase activity	1007:1030	myeloperoxidase activity	1007:1030	Gut permeability estimated by urinary chromium-EDTA excretion, bacterial translocation into mesenteric lymph nodes, myeloperoxidase activity, and expressions of the inflammatory cytokine genes in the cecal mucosa were greater in SP+FOS than in SP, but these alterations were not observed between NP and NP+FOS (experiment 1).
30381626	6	63	theme	genes	1078:1082	arg1	expressions					1037:1047	expressions	1037:1047	expressions of the inflammatory cytokine genes in the cecal mucosa	1037:1102	Gut permeability estimated by urinary chromium-EDTA excretion, bacterial translocation into mesenteric lymph nodes, myeloperoxidase activity, and expressions of the inflammatory cytokine genes in the cecal mucosa were greater in SP+FOS than in SP, but these alterations were not observed between NP and NP+FOS (experiment 1).
30381626	6	63	theme	genes	1078:1082	arg1	excretion					943:951	urinary chromium-EDTA excretion	921:951	urinary chromium-EDTA excretion	921:951	Gut permeability estimated by urinary chromium-EDTA excretion, bacterial translocation into mesenteric lymph nodes, myeloperoxidase activity, and expressions of the inflammatory cytokine genes in the cecal mucosa were greater in SP+FOS than in SP, but these alterations were not observed between NP and NP+FOS (experiment 1).
30381626	6	63	theme	genes	1078:1082	arg1	translocation					964:976	bacterial translocation	954:976	bacterial translocation into mesenteric lymph nodes	954:1004	Gut permeability estimated by urinary chromium-EDTA excretion, bacterial translocation into mesenteric lymph nodes, myeloperoxidase activity, and expressions of the inflammatory cytokine genes in the cecal mucosa were greater in SP+FOS than in SP, but these alterations were not observed between NP and NP+FOS (experiment 1).
30381626	4	64	theme	inflammatory	574:585	arg1	responses					587:595	inflammatory responses	574:595	inflammatory responses in the cecum (experiment 1)	574:623	We assessed microbial fermentation, gut permeability, and inflammatory responses in the cecum (experiment 1), and mucus layer in the cecum, intestinal transit time and microbiota composition (experiment 2).
30381626	1	65	from	effects	178:184	arg1	function					234:241	gut-barrier function	222:241	gut-barrier function	222:241	The effects of fructo-oligosaccharides (FOS) on gut-barrier function are still controversial in human and animal studies.
30381626	0	66	from	Impact	4:9	arg1	Permeability					45:56	Gut Permeability	41:56	Gut Permeability	41:56	The Impact of Fructo-Oligosaccharides on Gut Permeability and Inflammatory Responses in the Cecal Mucosa Quite Differs between Rats Fed Semi-Purified and Non-Purified Diets.
30381626	0	66	from	Impact	4:9	arg1	Responses					75:83	Inflammatory Responses	62:83	Inflammatory Responses in the Cecal Mucosa	62:103	The Impact of Fructo-Oligosaccharides on Gut Permeability and Inflammatory Responses in the Cecal Mucosa Quite Differs between Rats Fed Semi-Purified and Non-Purified Diets.
30381626	10	67	from	permeability	1561:1572	arg1	mucosa					1614:1619	the cecal mucosa	1604:1619	the cecal mucosa	1604:1619	These factors suggest that impact of FOS on gut permeability and inflammatory responses in the cecal mucosa quite differs between SP and NP.
30381626	3	68	dep	rats	382:385	arg1	experiment					501:510	experiment 2	501:512	experiment 2	501:512	We fed rats a semi-purified (SP) or a non-purified diet (NP) with or without FOS (60 g/kg diet) for 9 (experiment 1) or 10 d (experiment 2).
30381626	3	68	dep	rats	382:385	arg1	experiment					478:487	experiment 1	478:489	experiment 1	478:489	We fed rats a semi-purified (SP) or a non-purified diet (NP) with or without FOS (60 g/kg diet) for 9 (experiment 1) or 10 d (experiment 2).
30381626	3	68	dep	rats	382:385	arg1	diet					465:468	60 g/kg diet	457:468	60 g/kg diet	457:468	We fed rats a semi-purified (SP) or a non-purified diet (NP) with or without FOS (60 g/kg diet) for 9 (experiment 1) or 10 d (experiment 2).
30381626	3	68	dep	rats	382:385	arg1	rats					382:385	rats a semi-purified (SP) or a non-purified diet (NP) with or without FOS (60 g/kg diet) for 9 (experiment 1) or 10 d (experiment 2)	382:513	rats a semi-purified (SP) or a non-purified diet (NP) with or without FOS (60 g/kg diet) for 9 (experiment 1) or 10 d (experiment 2)	382:513	We fed rats a semi-purified (SP) or a non-purified diet (NP) with or without FOS (60 g/kg diet) for 9 (experiment 1) or 10 d (experiment 2).
30381626	3	68	dep	rats	382:385	arg1	diet					426:429	a non-purified diet	411:429	a non-purified diet (NP) with or without FOS (60 g/kg diet) for 9 (experiment 1)	411:490	We fed rats a semi-purified (SP) or a non-purified diet (NP) with or without FOS (60 g/kg diet) for 9 (experiment 1) or 10 d (experiment 2).
30381626	3	68	dep	rats	382:385	arg1	SP					404:405	SP	404:405	SP	404:405	We fed rats a semi-purified (SP) or a non-purified diet (NP) with or without FOS (60 g/kg diet) for 9 (experiment 1) or 10 d (experiment 2).
30381626	3	68	dep	rats	382:385	arg1	NP					432:433	NP	432:433	NP	432:433	We fed rats a semi-purified (SP) or a non-purified diet (NP) with or without FOS (60 g/kg diet) for 9 (experiment 1) or 10 d (experiment 2).
30381626	4	69	from	layer	636:640	arg1	experiment					611:620	experiment 1	611:622	experiment 1	611:622	We assessed microbial fermentation, gut permeability, and inflammatory responses in the cecum (experiment 1), and mucus layer in the cecum, intestinal transit time and microbiota composition (experiment 2).
30381626	4	69	from	layer	636:640	arg1	cecum					604:608	the cecum	600:608	the cecum (experiment 1)	600:623	We assessed microbial fermentation, gut permeability, and inflammatory responses in the cecum (experiment 1), and mucus layer in the cecum, intestinal transit time and microbiota composition (experiment 2).
30381626	3	70	theme	non-purified	413:424	arg1	rats					382:385	rats a semi-purified (SP) or a non-purified diet (NP) with or without FOS (60 g/kg diet) for 9 (experiment 1) or 10 d (experiment 2)	382:513	rats a semi-purified (SP) or a non-purified diet (NP) with or without FOS (60 g/kg diet) for 9 (experiment 1) or 10 d (experiment 2)	382:513	We fed rats a semi-purified (SP) or a non-purified diet (NP) with or without FOS (60 g/kg diet) for 9 (experiment 1) or 10 d (experiment 2).
30381626	3	70	theme	non-purified	413:424	arg1	experiment					478:487	experiment 1	478:489	experiment 1	478:489	We fed rats a semi-purified (SP) or a non-purified diet (NP) with or without FOS (60 g/kg diet) for 9 (experiment 1) or 10 d (experiment 2).
30381626	3	70	theme	non-purified	413:424	arg1	diet					465:468	60 g/kg diet	457:468	60 g/kg diet	457:468	We fed rats a semi-purified (SP) or a non-purified diet (NP) with or without FOS (60 g/kg diet) for 9 (experiment 1) or 10 d (experiment 2).
30381626	3	70	theme	non-purified	413:424	arg1	experiment					501:510	experiment 2	501:512	experiment 2	501:512	We fed rats a semi-purified (SP) or a non-purified diet (NP) with or without FOS (60 g/kg diet) for 9 (experiment 1) or 10 d (experiment 2).
30381626	3	70	theme	non-purified	413:424	arg1	diet					426:429	a non-purified diet	411:429	a non-purified diet (NP) with or without FOS (60 g/kg diet) for 9 (experiment 1)	411:490	We fed rats a semi-purified (SP) or a non-purified diet (NP) with or without FOS (60 g/kg diet) for 9 (experiment 1) or 10 d (experiment 2).
30381626	3	70	theme	non-purified	413:424	arg1	NP					432:433	NP	432:433	NP	432:433	We fed rats a semi-purified (SP) or a non-purified diet (NP) with or without FOS (60 g/kg diet) for 9 (experiment 1) or 10 d (experiment 2).
30381626	5	71	theme	lactate	805:811	arg1	accumulation					789:800	the accumulation	785:800	the accumulation of lactate and succinate in SP	785:831	FOS supplementation induced a very acidic fermentation due to the accumulation of lactate and succinate in SP, while short-chain fatty acids were major products in NP.
30381626	6	72	theme	chromium-EDTA	929:941	arg1	excretion					943:951	urinary chromium-EDTA excretion	921:951	urinary chromium-EDTA excretion	921:951	Gut permeability estimated by urinary chromium-EDTA excretion, bacterial translocation into mesenteric lymph nodes, myeloperoxidase activity, and expressions of the inflammatory cytokine genes in the cecal mucosa were greater in SP+FOS than in SP, but these alterations were not observed between NP and NP+FOS (experiment 1).
30381626	1	73	from	controversial	253:265	arg1	animal					280:285	animal	280:285	animal	280:285	The effects of fructo-oligosaccharides (FOS) on gut-barrier function are still controversial in human and animal studies.
30381626	1	73	from	controversial	253:265	arg1	human					270:274	human	270:274	human	270:274	The effects of fructo-oligosaccharides (FOS) on gut-barrier function are still controversial in human and animal studies.
30381626	3	74	dep	SP	404:405	arg1	a					387:387	a	387:387	a	387:387	We fed rats a semi-purified (SP) or a non-purified diet (NP) with or without FOS (60 g/kg diet) for 9 (experiment 1) or 10 d (experiment 2).
30381626	11	75	theme	contents	1785:1792	arg1	stasis					1751:1756	stasis	1751:1756	stasis of the very acidic luminal contents	1751:1792	Increased gut permeability in SP+FOS could be evoked by the disruption of the mucus layer due to stasis of the very acidic luminal contents.
30381626	0	76	theme	Fructo-Oligosaccharides	14:36	arg1	Impact					4:9	The Impact	0:9	The Impact of Fructo-Oligosaccharides on Gut Permeability and Inflammatory Responses in the Cecal Mucosa	0:103	The Impact of Fructo-Oligosaccharides on Gut Permeability and Inflammatory Responses in the Cecal Mucosa Quite Differs between Rats Fed Semi-Purified and Non-Purified Diets.
30381626	5	77	theme	succinate	817:825	arg1	accumulation					789:800	the accumulation	785:800	the accumulation of lactate and succinate in SP	785:831	FOS supplementation induced a very acidic fermentation due to the accumulation of lactate and succinate in SP, while short-chain fatty acids were major products in NP.
30381626	6	78	from	expressions	1037:1047	arg1	mucosa					1097:1102	the cecal mucosa	1087:1102	the cecal mucosa	1087:1102	Gut permeability estimated by urinary chromium-EDTA excretion, bacterial translocation into mesenteric lymph nodes, myeloperoxidase activity, and expressions of the inflammatory cytokine genes in the cecal mucosa were greater in SP+FOS than in SP, but these alterations were not observed between NP and NP+FOS (experiment 1).
30381626	3	79	theme	g/kg	460:463	arg1	diet					426:429	a non-purified diet	411:429	a non-purified diet (NP) with or without FOS (60 g/kg diet) for 9 (experiment 1)	411:490	We fed rats a semi-purified (SP) or a non-purified diet (NP) with or without FOS (60 g/kg diet) for 9 (experiment 1) or 10 d (experiment 2).
30381626	3	79	theme	g/kg	460:463	arg1	diet					465:468	60 g/kg diet	457:468	60 g/kg diet	457:468	We fed rats a semi-purified (SP) or a non-purified diet (NP) with or without FOS (60 g/kg diet) for 9 (experiment 1) or 10 d (experiment 2).
30381626	0	80	theme	Gut	41:43	arg1	Permeability					45:56	Gut Permeability	41:56	Gut Permeability	41:56	The Impact of Fructo-Oligosaccharides on Gut Permeability and Inflammatory Responses in the Cecal Mucosa Quite Differs between Rats Fed Semi-Purified and Non-Purified Diets.
30381626	6	81	theme	myeloperoxidase	1007:1021	arg1	activity					1023:1030	myeloperoxidase activity	1007:1030	myeloperoxidase activity	1007:1030	Gut permeability estimated by urinary chromium-EDTA excretion, bacterial translocation into mesenteric lymph nodes, myeloperoxidase activity, and expressions of the inflammatory cytokine genes in the cecal mucosa were greater in SP+FOS than in SP, but these alterations were not observed between NP and NP+FOS (experiment 1).
30381626	3	82	theme	semi-purified	389:401	arg1	rats					382:385	rats a semi-purified (SP) or a non-purified diet (NP) with or without FOS (60 g/kg diet) for 9 (experiment 1) or 10 d (experiment 2)	382:513	rats a semi-purified (SP) or a non-purified diet (NP) with or without FOS (60 g/kg diet) for 9 (experiment 1) or 10 d (experiment 2)	382:513	We fed rats a semi-purified (SP) or a non-purified diet (NP) with or without FOS (60 g/kg diet) for 9 (experiment 1) or 10 d (experiment 2).
30381626	3	82	theme	semi-purified	389:401	arg1	SP					404:405	SP	404:405	SP	404:405	We fed rats a semi-purified (SP) or a non-purified diet (NP) with or without FOS (60 g/kg diet) for 9 (experiment 1) or 10 d (experiment 2).
30381626	5	83	theme	fatty	852:856	arg1	acids					858:862	short-chain fatty acids	840:862	short-chain fatty acids	840:862	FOS supplementation induced a very acidic fermentation due to the accumulation of lactate and succinate in SP, while short-chain fatty acids were major products in NP.
30381626	6	84	from	translocation	964:976	arg1	mucosa					1097:1102	the cecal mucosa	1087:1102	the cecal mucosa	1087:1102	Gut permeability estimated by urinary chromium-EDTA excretion, bacterial translocation into mesenteric lymph nodes, myeloperoxidase activity, and expressions of the inflammatory cytokine genes in the cecal mucosa were greater in SP+FOS than in SP, but these alterations were not observed between NP and NP+FOS (experiment 1).
30381626	2	85	theme	major	323:327	arg1	factor					329:334	a major factor	321:334	a major factor for the controversy in animal studies	321:372	Diet conditions would be a major factor for the controversy in animal studies.
30381626	2	85	theme	major	323:327	arg1	conditions					301:310	Diet conditions	296:310	Diet conditions	296:310	Diet conditions would be a major factor for the controversy in animal studies.
30381626	9	86	theme	species	1430:1436	arg1	richness					1438:1445	Lower species richness	1424:1445	Lower species richness of cecal microbiota	1424:1465	Lower species richness of cecal microbiota was manifest solely in SP+FOS (experiment 2).
30381626	6	87	theme	mesenteric	983:992	arg1	nodes					1000:1004	mesenteric lymph nodes	983:1004	mesenteric lymph nodes	983:1004	Gut permeability estimated by urinary chromium-EDTA excretion, bacterial translocation into mesenteric lymph nodes, myeloperoxidase activity, and expressions of the inflammatory cytokine genes in the cecal mucosa were greater in SP+FOS than in SP, but these alterations were not observed between NP and NP+FOS (experiment 1).
30381626	4	88	theme	transit	667:673	arg1	time					675:678	intestinal transit time	656:678	intestinal transit time	656:678	We assessed microbial fermentation, gut permeability, and inflammatory responses in the cecum (experiment 1), and mucus layer in the cecum, intestinal transit time and microbiota composition (experiment 2).
30381626	2	89	theme	animal	359:364	arg1	studies					366:372	animal studies	359:372	animal studies	359:372	Diet conditions would be a major factor for the controversy in animal studies.
29873488	2	0	theme	gut	430:432	arg1	microbiota					434:443	gut microbiota	430:443	gut microbiota	430:443	We compared nine chicory genotypes in order to determine if variations in the content of metabolites in the roasted roots would lead to modifications in release of satiety hormones and in composition of gut microbiota.
29873488	5	1	theme	chicory	727:733	arg1	digests					746:752	chicory intestinal digests	727:752	chicory intestinal digests	727:752	In vitro gastrointestinal digestions were performed in order to study the effect of chicory intestinal digests on gut hormone regulation in enteroendocrine cells.
29873488	1	2	theme	prebiotic	124:132	arg1	effects					134:140	the prebiotic effects	120:140	the prebiotic effects of chicory ( Cichorium intybus) together with its possible role in appetite control	120:224	The objectives of this work are to address the prebiotic effects of chicory ( Cichorium intybus) together with its possible role in appetite control.
29873488	1	3	theme	appetite	209:216	arg1	control					218:224	appetite control	209:224	appetite control	209:224	The objectives of this work are to address the prebiotic effects of chicory ( Cichorium intybus) together with its possible role in appetite control.
29873488	5	4	dep	In	643:644	arg1	vitro					646:650	vitro	646:650	vitro	646:650	In vitro gastrointestinal digestions were performed in order to study the effect of chicory intestinal digests on gut hormone regulation in enteroendocrine cells.
29873488	6	5	theme	bacterial	845:853	arg1	Oscillibacter					911:923	Oscillibacter	911:923	Oscillibacter	911:923	Firmicutes/Bacteroidetes ratio and gut bacterial groups, such as Alloprevotella, Blautia, Alistipes, and Oscillibacter, were found to be modulated by chicory.
29873488	6	5	theme	bacterial	845:853	arg1	Blautia					887:893	Blautia	887:893	Blautia	887:893	Firmicutes/Bacteroidetes ratio and gut bacterial groups, such as Alloprevotella, Blautia, Alistipes, and Oscillibacter, were found to be modulated by chicory.
29873488	6	5	theme	bacterial	845:853	arg1	Alistipes					896:904	Alistipes	896:904	Alistipes	896:904	Firmicutes/Bacteroidetes ratio and gut bacterial groups, such as Alloprevotella, Blautia, Alistipes, and Oscillibacter, were found to be modulated by chicory.
29873488	6	5	theme	bacterial	845:853	arg1	groups					855:860	Firmicutes/Bacteroidetes ratio and gut bacterial groups	806:860	Firmicutes/Bacteroidetes ratio and gut bacterial groups	806:860	Firmicutes/Bacteroidetes ratio and gut bacterial groups, such as Alloprevotella, Blautia, Alistipes, and Oscillibacter, were found to be modulated by chicory.
29873488	2	6	theme	chicory	244:250	arg1	genotypes					252:260	nine chicory genotypes	239:260	nine chicory genotypes in order to determine if variations in the content of metabolites in the roasted roots would lead to modifications in release of satiety hormones and in composition of gut microbiota	239:443	We compared nine chicory genotypes in order to determine if variations in the content of metabolites in the roasted roots would lead to modifications in release of satiety hormones and in composition of gut microbiota.
29873488	2	7	from	variations	287:296	arg1	content					305:311	the content	301:311	the content of metabolites in the roasted roots	301:347	We compared nine chicory genotypes in order to determine if variations in the content of metabolites in the roasted roots would lead to modifications in release of satiety hormones and in composition of gut microbiota.
29873488	4	8	theme	fecal	611:615	arg1	microbiota					617:626	fecal microbiota	611:626	fecal microbiota	611:626	A 16S rRNA gene-based metagenetic analysis of fecal microbiota was performed.
29873488	5	9	theme	gut	757:759	arg1	regulation					769:778	gut hormone regulation	757:778	gut hormone regulation in enteroendocrine cells	757:803	In vitro gastrointestinal digestions were performed in order to study the effect of chicory intestinal digests on gut hormone regulation in enteroendocrine cells.
29873488	2	10	from	content	305:311	arg1	roots					343:347	the roasted roots	331:347	the roasted roots	331:347	We compared nine chicory genotypes in order to determine if variations in the content of metabolites in the roasted roots would lead to modifications in release of satiety hormones and in composition of gut microbiota.
29873488	3	11	theme	5-week	461:466	arg1	study					489:493	a 5-week dietary-intervention study	459:493	a 5-week dietary-intervention study	459:493	To this aim, a 5-week dietary-intervention study was achieved using mice fed with distinct chicory-based preparations.
29873488	2	12	theme	roasted	335:341	arg1	roots					343:347	the roasted roots	331:347	the roasted roots	331:347	We compared nine chicory genotypes in order to determine if variations in the content of metabolites in the roasted roots would lead to modifications in release of satiety hormones and in composition of gut microbiota.
29873488	2	13	theme	satiety	391:397	arg1	hormones					399:406	satiety hormones	391:406	satiety hormones	391:406	We compared nine chicory genotypes in order to determine if variations in the content of metabolites in the roasted roots would lead to modifications in release of satiety hormones and in composition of gut microbiota.
29873488	5	14	from	regulation	769:778	arg1	cells					799:803	enteroendocrine cells	783:803	enteroendocrine cells	783:803	In vitro gastrointestinal digestions were performed in order to study the effect of chicory intestinal digests on gut hormone regulation in enteroendocrine cells.
29873488	7	15	theme	GLP-1	992:996	arg1	hormones					1006:1013	CCK and GLP-1 satiety hormones	984:1013	CCK and GLP-1 satiety hormones	984:1013	On the other hand, CCK and GLP-1 satiety hormones were demonstrated to be significantly increased by chicory in vitro.
29873488	1	16	theme	chicory	145:151	arg1	effects					134:140	the prebiotic effects	120:140	the prebiotic effects of chicory ( Cichorium intybus) together with its possible role in appetite control	120:224	The objectives of this work are to address the prebiotic effects of chicory ( Cichorium intybus) together with its possible role in appetite control.
29873488	0	17	theme	Chicory	0:6	arg1	Roots					8:12	Chicory Roots	0:12	Chicory Roots for Prebiotics and Appetite Regulation: A Pilot Study in Mice.	0:75	Chicory Roots for Prebiotics and Appetite Regulation: A Pilot Study in Mice.
29873488	4	18	theme	microbiota	617:626	arg1	analysis					599:606	A 16S rRNA gene-based metagenetic analysis	565:606	A 16S rRNA gene-based metagenetic analysis of fecal microbiota	565:626	A 16S rRNA gene-based metagenetic analysis of fecal microbiota was performed.
29873488	5	19	theme	gastrointestinal	652:667	arg1	digestions					669:678	In vitro gastrointestinal digestions	643:678	In vitro gastrointestinal digestions	643:678	In vitro gastrointestinal digestions were performed in order to study the effect of chicory intestinal digests on gut hormone regulation in enteroendocrine cells.
29873488	3	20	theme	distinct	528:535	arg1	preparations					551:562	distinct chicory-based preparations	528:562	distinct chicory-based preparations	528:562	To this aim, a 5-week dietary-intervention study was achieved using mice fed with distinct chicory-based preparations.
29873488	4	21	theme	gene-based	576:585	arg1	analysis					599:606	A 16S rRNA gene-based metagenetic analysis	565:606	A 16S rRNA gene-based metagenetic analysis of fecal microbiota	565:626	A 16S rRNA gene-based metagenetic analysis of fecal microbiota was performed.
29873488	5	22	theme	enteroendocrine	783:797	arg1	cells					799:803	enteroendocrine cells	783:803	enteroendocrine cells	783:803	In vitro gastrointestinal digestions were performed in order to study the effect of chicory intestinal digests on gut hormone regulation in enteroendocrine cells.
29873488	4	23	theme	rRNA	571:574	arg1	analysis					599:606	A 16S rRNA gene-based metagenetic analysis	565:606	A 16S rRNA gene-based metagenetic analysis of fecal microbiota	565:626	A 16S rRNA gene-based metagenetic analysis of fecal microbiota was performed.
29873488	5	24	theme	intestinal	735:744	arg1	digests					746:752	chicory intestinal digests	727:752	chicory intestinal digests	727:752	In vitro gastrointestinal digestions were performed in order to study the effect of chicory intestinal digests on gut hormone regulation in enteroendocrine cells.
29873488	7	25	theme	satiety	998:1004	arg1	hormones					1006:1013	CCK and GLP-1 satiety hormones	984:1013	CCK and GLP-1 satiety hormones	984:1013	On the other hand, CCK and GLP-1 satiety hormones were demonstrated to be significantly increased by chicory in vitro.
29873488	0	26	from	Study	62:66	arg1	Mice					71:74	Mice	71:74	Mice	71:74	Chicory Roots for Prebiotics and Appetite Regulation: A Pilot Study in Mice.
29873488	1	27	dep	chicory	145:151	arg1	intybus					165:171	Cichorium intybus	155:171	Cichorium intybus	155:171	The objectives of this work are to address the prebiotic effects of chicory ( Cichorium intybus) together with its possible role in appetite control.
29873488	5	28	theme	digests	746:752	arg1	effect					717:722	the effect	713:722	the effect of chicory intestinal digests on gut hormone regulation in enteroendocrine cells	713:803	In vitro gastrointestinal digestions were performed in order to study the effect of chicory intestinal digests on gut hormone regulation in enteroendocrine cells.
29873488	6	29	theme	gut	841:843	arg1	Oscillibacter					911:923	Oscillibacter	911:923	Oscillibacter	911:923	Firmicutes/Bacteroidetes ratio and gut bacterial groups, such as Alloprevotella, Blautia, Alistipes, and Oscillibacter, were found to be modulated by chicory.
29873488	6	29	theme	gut	841:843	arg1	Blautia					887:893	Blautia	887:893	Blautia	887:893	Firmicutes/Bacteroidetes ratio and gut bacterial groups, such as Alloprevotella, Blautia, Alistipes, and Oscillibacter, were found to be modulated by chicory.
29873488	6	29	theme	gut	841:843	arg1	Alistipes					896:904	Alistipes	896:904	Alistipes	896:904	Firmicutes/Bacteroidetes ratio and gut bacterial groups, such as Alloprevotella, Blautia, Alistipes, and Oscillibacter, were found to be modulated by chicory.
29873488	6	29	theme	gut	841:843	arg1	groups					855:860	Firmicutes/Bacteroidetes ratio and gut bacterial groups	806:860	Firmicutes/Bacteroidetes ratio and gut bacterial groups	806:860	Firmicutes/Bacteroidetes ratio and gut bacterial groups, such as Alloprevotella, Blautia, Alistipes, and Oscillibacter, were found to be modulated by chicory.
29873488	3	30	theme	chicory-based	537:549	arg1	preparations					551:562	distinct chicory-based preparations	528:562	distinct chicory-based preparations	528:562	To this aim, a 5-week dietary-intervention study was achieved using mice fed with distinct chicory-based preparations.
29873488	7	31	theme	other	972:976	arg1	hand					978:981	the other hand	968:981	the other hand	968:981	On the other hand, CCK and GLP-1 satiety hormones were demonstrated to be significantly increased by chicory in vitro.
29873488	4	32	theme	metagenetic	587:597	arg1	analysis					599:606	A 16S rRNA gene-based metagenetic analysis	565:606	A 16S rRNA gene-based metagenetic analysis of fecal microbiota	565:626	A 16S rRNA gene-based metagenetic analysis of fecal microbiota was performed.
29873488	2	33	theme	hormones	399:406	arg1	release					380:386	release	380:386	release of satiety hormones	380:406	We compared nine chicory genotypes in order to determine if variations in the content of metabolites in the roasted roots would lead to modifications in release of satiety hormones and in composition of gut microbiota.
29873488	1	34	from	role	201:204	arg1	control					218:224	appetite control	209:224	appetite control	209:224	The objectives of this work are to address the prebiotic effects of chicory ( Cichorium intybus) together with its possible role in appetite control.
29873488	6	35	theme	ratio	831:835	arg1	Oscillibacter					911:923	Oscillibacter	911:923	Oscillibacter	911:923	Firmicutes/Bacteroidetes ratio and gut bacterial groups, such as Alloprevotella, Blautia, Alistipes, and Oscillibacter, were found to be modulated by chicory.
29873488	6	35	theme	ratio	831:835	arg1	Blautia					887:893	Blautia	887:893	Blautia	887:893	Firmicutes/Bacteroidetes ratio and gut bacterial groups, such as Alloprevotella, Blautia, Alistipes, and Oscillibacter, were found to be modulated by chicory.
29873488	6	35	theme	ratio	831:835	arg1	Alistipes					896:904	Alistipes	896:904	Alistipes	896:904	Firmicutes/Bacteroidetes ratio and gut bacterial groups, such as Alloprevotella, Blautia, Alistipes, and Oscillibacter, were found to be modulated by chicory.
29873488	6	35	theme	ratio	831:835	arg1	groups					855:860	Firmicutes/Bacteroidetes ratio and gut bacterial groups	806:860	Firmicutes/Bacteroidetes ratio and gut bacterial groups	806:860	Firmicutes/Bacteroidetes ratio and gut bacterial groups, such as Alloprevotella, Blautia, Alistipes, and Oscillibacter, were found to be modulated by chicory.
29873488	5	36	theme	In	643:644	arg1	digestions					669:678	In vitro gastrointestinal digestions	643:678	In vitro gastrointestinal digestions	643:678	In vitro gastrointestinal digestions were performed in order to study the effect of chicory intestinal digests on gut hormone regulation in enteroendocrine cells.
29873488	6	37	theme	Firmicutes/Bacteroidetes	806:829	arg1	ratio					831:835	Firmicutes/Bacteroidetes ratio	806:835	Firmicutes/Bacteroidetes ratio	806:835	Firmicutes/Bacteroidetes ratio and gut bacterial groups, such as Alloprevotella, Blautia, Alistipes, and Oscillibacter, were found to be modulated by chicory.
29873488	0	38	dep	Prebiotics	18:27	arg1	Regulation					42:51	Regulation	42:51	Regulation	42:51	Chicory Roots for Prebiotics and Appetite Regulation: A Pilot Study in Mice.
29873488	1	39	theme	work	100:103	arg1	objectives					81:90	The objectives	77:90	The objectives of this work	77:103	The objectives of this work are to address the prebiotic effects of chicory ( Cichorium intybus) together with its possible role in appetite control.
29873488	7	40	theme	CCK	984:986	arg1	hormones					1006:1013	CCK and GLP-1 satiety hormones	984:1013	CCK and GLP-1 satiety hormones	984:1013	On the other hand, CCK and GLP-1 satiety hormones were demonstrated to be significantly increased by chicory in vitro.
29873488	3	41	theme	dietary-intervention	468:487	arg1	study					489:493	a 5-week dietary-intervention study	459:493	a 5-week dietary-intervention study	459:493	To this aim, a 5-week dietary-intervention study was achieved using mice fed with distinct chicory-based preparations.
29873488	0	42	theme	Pilot	56:60	arg1	Study					62:66	A Pilot Study	54:66	Chicory Roots for Prebiotics and Appetite Regulation: A Pilot Study in Mice.	0:75	Chicory Roots for Prebiotics and Appetite Regulation: A Pilot Study in Mice.
29873488	5	43	from	effect	717:722	arg1	regulation					769:778	gut hormone regulation	757:778	gut hormone regulation in enteroendocrine cells	757:803	In vitro gastrointestinal digestions were performed in order to study the effect of chicory intestinal digests on gut hormone regulation in enteroendocrine cells.
29873488	0	44	dep	Roots	8:12	arg1	Study					62:66	A Pilot Study	54:66	Chicory Roots for Prebiotics and Appetite Regulation: A Pilot Study in Mice.	0:75	Chicory Roots for Prebiotics and Appetite Regulation: A Pilot Study in Mice.
29873488	4	45	theme	16S	567:569	arg1	analysis					599:606	A 16S rRNA gene-based metagenetic analysis	565:606	A 16S rRNA gene-based metagenetic analysis of fecal microbiota	565:626	A 16S rRNA gene-based metagenetic analysis of fecal microbiota was performed.
29873488	2	46	theme	metabolites	316:326	arg1	content					305:311	the content	301:311	the content of metabolites in the roasted roots	301:347	We compared nine chicory genotypes in order to determine if variations in the content of metabolites in the roasted roots would lead to modifications in release of satiety hormones and in composition of gut microbiota.
29873488	1	47	theme	possible	192:199	arg1	role					201:204	its possible role	188:204	its possible role in appetite control	188:224	The objectives of this work are to address the prebiotic effects of chicory ( Cichorium intybus) together with its possible role in appetite control.
29873488	2	48	theme	microbiota	434:443	arg1	composition					415:425	composition	415:425	composition of gut microbiota	415:443	We compared nine chicory genotypes in order to determine if variations in the content of metabolites in the roasted roots would lead to modifications in release of satiety hormones and in composition of gut microbiota.
29873488	2	49	from	modifications	363:375	arg1	release					380:386	release	380:386	release of satiety hormones	380:406	We compared nine chicory genotypes in order to determine if variations in the content of metabolites in the roasted roots would lead to modifications in release of satiety hormones and in composition of gut microbiota.
29873488	2	49	from	modifications	363:375	arg1	composition					415:425	composition	415:425	composition of gut microbiota	415:443	We compared nine chicory genotypes in order to determine if variations in the content of metabolites in the roasted roots would lead to modifications in release of satiety hormones and in composition of gut microbiota.
29873488	5	50	theme	hormone	761:767	arg1	regulation					769:778	gut hormone regulation	757:778	gut hormone regulation in enteroendocrine cells	757:803	In vitro gastrointestinal digestions were performed in order to study the effect of chicory intestinal digests on gut hormone regulation in enteroendocrine cells.
30429436	4	0	theme	cell	812:815	arg1	wall					817:820	the yeast cell wall	802:820	the yeast cell wall	802:820	HI Candida and the mannan exhibited different effects on BMDC activation and functions, which could be due to other carbohydrate compositions in the yeast cell wall.
30429436	6	1	theme	helper	1006:1011	arg1	responses					1018:1026	T helper (Th) responses	1004:1026	T helper (Th) responses	1004:1026	This suggests the possibility that C. albicans mannan may skew T helper (Th) responses from protective Th17 toward Th1.
30429436	3	2	from	effects	467:473	arg1	responses					632:640	bone marrow-derived DC (BMDC) responses	602:640	bone marrow-derived DC (BMDC) responses	602:640	The effects of heat-inactivated (HI) yeast cells and soluble mannan of Candida albicans, Candida parapsilosis, and Candida dubliniensis on bone marrow-derived DC (BMDC) responses were compared.
30429436	3	3	link	marrow-derived	607:620	arg1	BMDC					626:629	BMDC	626:629	BMDC	626:629	The effects of heat-inactivated (HI) yeast cells and soluble mannan of Candida albicans, Candida parapsilosis, and Candida dubliniensis on bone marrow-derived DC (BMDC) responses were compared.
30429436	3	3	link	marrow-derived	607:620	arg1	DC					622:623	bone marrow-derived DC	602:623	bone marrow-derived DC (BMDC) responses	602:640	The effects of heat-inactivated (HI) yeast cells and soluble mannan of Candida albicans, Candida parapsilosis, and Candida dubliniensis on bone marrow-derived DC (BMDC) responses were compared.
30429436	3	4	theme	cells	506:510	arg1	mannan					524:529	soluble mannan	516:529	soluble mannan	516:529	The effects of heat-inactivated (HI) yeast cells and soluble mannan of Candida albicans, Candida parapsilosis, and Candida dubliniensis on bone marrow-derived DC (BMDC) responses were compared.
30429436	3	4	theme	cells	506:510	arg1	effects					467:473	The effects	463:473	The effects of heat-inactivated (HI) yeast cells	463:510	The effects of heat-inactivated (HI) yeast cells and soluble mannan of Candida albicans, Candida parapsilosis, and Candida dubliniensis on bone marrow-derived DC (BMDC) responses were compared.
30429436	7	5	theme	C.	1074:1075	arg1	mannan					1090:1095	C. parapsilosis mannan	1074:1095	C. parapsilosis mannan	1074:1095	In contrast, C. parapsilosis mannan caused strong BMDC activation and high production of several proinflammatory cytokines which possibly promote hyperinflammation.
30429436	9	6	theme	Candida	1369:1375	arg1	species					1377:1383	each Candida species	1364:1383	each Candida species	1364:1383	Therefore, mannan of each Candida species play distinct roles in DC responses and may be involved in the immunopathogenesis and disease severity of oral candidiasis as well as other Candida infection.
30429436	0	7	theme	Candida	105:111	arg1	dubliniensis					113:124	Candida dubliniensis	105:124	Candida dubliniensis	105:124	Differential dendritic cell responses to cell wall mannan of Candida albicans, Candida parapsilosis, and Candida dubliniensis.
30429436	3	8	theme	soluble	516:522	arg1	mannan					524:529	soluble mannan	516:529	soluble mannan	516:529	The effects of heat-inactivated (HI) yeast cells and soluble mannan of Candida albicans, Candida parapsilosis, and Candida dubliniensis on bone marrow-derived DC (BMDC) responses were compared.
30429436	3	9	theme	Candida	552:558	arg1	parapsilosis					560:571	Candida parapsilosis	552:571	Candida parapsilosis	552:571	The effects of heat-inactivated (HI) yeast cells and soluble mannan of Candida albicans, Candida parapsilosis, and Candida dubliniensis on bone marrow-derived DC (BMDC) responses were compared.
30429436	1	10	theme	cell	201:204	arg1	wall					206:209	the Candida cell wall	189:209	the Candida cell wall	189:209	Mannan (mannosylated glycoproteins) in the outermost layer of the Candida cell wall may be the first molecules that interact with host dendritic cells (DCs) and activate immune responses that determine disease outcomes.
30429436	3	11	theme	albicans	542:549	arg1	mannan					524:529	soluble mannan	516:529	soluble mannan	516:529	The effects of heat-inactivated (HI) yeast cells and soluble mannan of Candida albicans, Candida parapsilosis, and Candida dubliniensis on bone marrow-derived DC (BMDC) responses were compared.
30429436	3	11	theme	albicans	542:549	arg1	effects					467:473	The effects	463:473	The effects of heat-inactivated (HI) yeast cells	463:510	The effects of heat-inactivated (HI) yeast cells and soluble mannan of Candida albicans, Candida parapsilosis, and Candida dubliniensis on bone marrow-derived DC (BMDC) responses were compared.
30429436	5	12	theme	C.	849:850	arg1	stimulus					884:891	the weakest stimulus	872:891	the weakest stimulus	872:891	Among Candida mannan, the C. albicans mannan was the weakest stimulus and induced only interferon (IFN)-γ production.
30429436	5	12	theme	C.	849:850	arg1	mannan					861:866	the C. albicans mannan	845:866	the C. albicans mannan	845:866	Among Candida mannan, the C. albicans mannan was the weakest stimulus and induced only interferon (IFN)-γ production.
30429436	5	13	theme	weakest	876:882	arg1	stimulus					884:891	the weakest stimulus	872:891	the weakest stimulus	872:891	Among Candida mannan, the C. albicans mannan was the weakest stimulus and induced only interferon (IFN)-γ production.
30429436	5	13	theme	weakest	876:882	arg1	mannan					861:866	the C. albicans mannan	845:866	the C. albicans mannan	845:866	Among Candida mannan, the C. albicans mannan was the weakest stimulus and induced only interferon (IFN)-γ production.
30429436	1	14	gly	glycoproteins	148:160	arg1	Mannan					127:132	Mannan	127:132	Mannan (mannosylated glycoproteins) in the outermost layer of the Candida cell wall	127:209	Mannan (mannosylated glycoproteins) in the outermost layer of the Candida cell wall may be the first molecules that interact with host dendritic cells (DCs) and activate immune responses that determine disease outcomes.
30429436	1	14	gly	glycoproteins	148:160	arg1	glycoproteins					148:160	mannosylated glycoproteins	135:160	mannosylated glycoproteins	135:160	Mannan (mannosylated glycoproteins) in the outermost layer of the Candida cell wall may be the first molecules that interact with host dendritic cells (DCs) and activate immune responses that determine disease outcomes.
30429436	3	15	theme	Candida	578:584	arg1	dubliniensis					586:597	Candida dubliniensis	578:597	Candida dubliniensis	578:597	The effects of heat-inactivated (HI) yeast cells and soluble mannan of Candida albicans, Candida parapsilosis, and Candida dubliniensis on bone marrow-derived DC (BMDC) responses were compared.
30429436	7	16	theme	high	1131:1134	arg1	production					1136:1145	high production	1131:1145	high production of several proinflammatory cytokines which possibly promote hyperinflammation	1131:1223	In contrast, C. parapsilosis mannan caused strong BMDC activation and high production of several proinflammatory cytokines which possibly promote hyperinflammation.
30429436	5	17	theme	Candida	829:835	arg1	mannan					837:842	Candida mannan	829:842	Candida mannan	829:842	Among Candida mannan, the C. albicans mannan was the weakest stimulus and induced only interferon (IFN)-γ production.
30429436	0	18	theme	albicans	69:76	arg1	mannan					51:56	cell wall mannan	41:56	cell wall mannan of Candida albicans, Candida parapsilosis, and Candida dubliniensis	41:124	Differential dendritic cell responses to cell wall mannan of Candida albicans, Candida parapsilosis, and Candida dubliniensis.
30429436	8	19	theme	BMDC	1277:1280	arg1	responses					1282:1290	moderate BMDC responses	1268:1290	moderate BMDC responses	1268:1290	Meanwhile, C. dubliniensis mannan induced moderate BMDC responses, which may correlate with its lower pathogenicity.
30429436	0	20	theme	Candida	79:85	arg1	parapsilosis					87:98	Candida parapsilosis	79:98	Candida parapsilosis	79:98	Differential dendritic cell responses to cell wall mannan of Candida albicans, Candida parapsilosis, and Candida dubliniensis.
30429436	9	21	theme	disease	1471:1477	arg1	severity					1479:1486	disease severity	1471:1486	disease severity	1471:1486	Therefore, mannan of each Candida species play distinct roles in DC responses and may be involved in the immunopathogenesis and disease severity of oral candidiasis as well as other Candida infection.
30429436	6	22	dep	C.	976:977	arg1	albicans					979:986	albicans	979:986	albicans	979:986	This suggests the possibility that C. albicans mannan may skew T helper (Th) responses from protective Th17 toward Th1.
30429436	4	23	from	compositions	786:797	arg1	wall					817:820	the yeast cell wall	802:820	the yeast cell wall	802:820	HI Candida and the mannan exhibited different effects on BMDC activation and functions, which could be due to other carbohydrate compositions in the yeast cell wall.
30429436	4	24	from	effects	703:709	arg1	functions					734:742	functions	734:742	functions	734:742	HI Candida and the mannan exhibited different effects on BMDC activation and functions, which could be due to other carbohydrate compositions in the yeast cell wall.
30429436	4	24	from	effects	703:709	arg1	activation					719:728	BMDC activation	714:728	BMDC activation	714:728	HI Candida and the mannan exhibited different effects on BMDC activation and functions, which could be due to other carbohydrate compositions in the yeast cell wall.
30429436	5	25	theme	interferon	910:919	arg1	production					929:938	only interferon (IFN)-γ production	905:938	only interferon (IFN)-γ production	905:938	Among Candida mannan, the C. albicans mannan was the weakest stimulus and induced only interferon (IFN)-γ production.
30429436	2	26	theme	common	413:418	arg1	species					433:439	common oral Candida species	413:439	common oral Candida species	413:439	However, little is known about how different mannan structures of common oral Candida species affect DC activation.
30429436	0	27	theme	Differential	0:11	arg1	responses					28:36	Differential dendritic cell responses	0:36	Differential dendritic cell responses to cell wall mannan of Candida albicans, Candida parapsilosis, and Candida dubliniensis	0:124	Differential dendritic cell responses to cell wall mannan of Candida albicans, Candida parapsilosis, and Candida dubliniensis.
30429436	9	28	theme	candidiasis	1496:1506	arg1	severity					1479:1486	disease severity	1471:1486	disease severity	1471:1486	Therefore, mannan of each Candida species play distinct roles in DC responses and may be involved in the immunopathogenesis and disease severity of oral candidiasis as well as other Candida infection.
30429436	9	28	theme	candidiasis	1496:1506	arg1	immunopathogenesis					1448:1465	immunopathogenesis	1448:1465	immunopathogenesis	1448:1465	Therefore, mannan of each Candida species play distinct roles in DC responses and may be involved in the immunopathogenesis and disease severity of oral candidiasis as well as other Candida infection.
30429436	2	29	theme	different	382:390	arg1	structures					399:408	different mannan structures	382:408	different mannan structures of common oral Candida species	382:439	However, little is known about how different mannan structures of common oral Candida species affect DC activation.
30429436	0	30	theme	cell	23:26	arg1	responses					28:36	Differential dendritic cell responses	0:36	Differential dendritic cell responses to cell wall mannan of Candida albicans, Candida parapsilosis, and Candida dubliniensis	0:124	Differential dendritic cell responses to cell wall mannan of Candida albicans, Candida parapsilosis, and Candida dubliniensis.
30429436	1	31	theme	dendritic	262:270	arg1	DCs					279:281	DCs	279:281	DCs	279:281	Mannan (mannosylated glycoproteins) in the outermost layer of the Candida cell wall may be the first molecules that interact with host dendritic cells (DCs) and activate immune responses that determine disease outcomes.
30429436	1	31	theme	dendritic	262:270	arg1	cells					272:276	host dendritic cells	257:276	host dendritic cells (DCs)	257:282	Mannan (mannosylated glycoproteins) in the outermost layer of the Candida cell wall may be the first molecules that interact with host dendritic cells (DCs) and activate immune responses that determine disease outcomes.
30429436	3	32	theme	marrow-derived	607:620	arg1	BMDC					626:629	BMDC	626:629	BMDC	626:629	The effects of heat-inactivated (HI) yeast cells and soluble mannan of Candida albicans, Candida parapsilosis, and Candida dubliniensis on bone marrow-derived DC (BMDC) responses were compared.
30429436	3	32	theme	marrow-derived	607:620	arg1	DC					622:623	bone marrow-derived DC	602:623	bone marrow-derived DC (BMDC) responses	602:640	The effects of heat-inactivated (HI) yeast cells and soluble mannan of Candida albicans, Candida parapsilosis, and Candida dubliniensis on bone marrow-derived DC (BMDC) responses were compared.
30429436	8	33	theme	lower	1322:1326	arg1	pathogenicity					1328:1340	its lower pathogenicity	1318:1340	its lower pathogenicity	1318:1340	Meanwhile, C. dubliniensis mannan induced moderate BMDC responses, which may correlate with its lower pathogenicity.
30429436	9	34	theme	other	1519:1523	arg1	infection					1533:1541	other Candida infection	1519:1541	oral candidiasis as well as other Candida infection	1491:1541	Therefore, mannan of each Candida species play distinct roles in DC responses and may be involved in the immunopathogenesis and disease severity of oral candidiasis as well as other Candida infection.
30429436	2	35	theme	Candida	425:431	arg1	species					433:439	common oral Candida species	413:439	common oral Candida species	413:439	However, little is known about how different mannan structures of common oral Candida species affect DC activation.
30429436	4	36	theme	other	767:771	arg1	compositions					786:797	other carbohydrate compositions	767:797	other carbohydrate compositions in the yeast cell wall	767:820	HI Candida and the mannan exhibited different effects on BMDC activation and functions, which could be due to other carbohydrate compositions in the yeast cell wall.
30429436	0	37	theme	wall	46:49	arg1	mannan					51:56	cell wall mannan	41:56	cell wall mannan of Candida albicans, Candida parapsilosis, and Candida dubliniensis	41:124	Differential dendritic cell responses to cell wall mannan of Candida albicans, Candida parapsilosis, and Candida dubliniensis.
30429436	9	38	theme	infection	1533:1541	arg1	severity					1479:1486	disease severity	1471:1486	disease severity	1471:1486	Therefore, mannan of each Candida species play distinct roles in DC responses and may be involved in the immunopathogenesis and disease severity of oral candidiasis as well as other Candida infection.
30429436	9	38	theme	infection	1533:1541	arg1	immunopathogenesis					1448:1465	immunopathogenesis	1448:1465	immunopathogenesis	1448:1465	Therefore, mannan of each Candida species play distinct roles in DC responses and may be involved in the immunopathogenesis and disease severity of oral candidiasis as well as other Candida infection.
30429436	1	39	theme	wall	206:209	arg1	layer					180:184	the outermost layer	166:184	the outermost layer of the Candida cell wall	166:209	Mannan (mannosylated glycoproteins) in the outermost layer of the Candida cell wall may be the first molecules that interact with host dendritic cells (DCs) and activate immune responses that determine disease outcomes.
30429436	1	40	theme	mannosylated	135:146	arg1	Mannan					127:132	Mannan	127:132	Mannan (mannosylated glycoproteins) in the outermost layer of the Candida cell wall	127:209	Mannan (mannosylated glycoproteins) in the outermost layer of the Candida cell wall may be the first molecules that interact with host dendritic cells (DCs) and activate immune responses that determine disease outcomes.
30429436	1	40	theme	mannosylated	135:146	arg1	glycoproteins					148:160	mannosylated glycoproteins	135:160	mannosylated glycoproteins	135:160	Mannan (mannosylated glycoproteins) in the outermost layer of the Candida cell wall may be the first molecules that interact with host dendritic cells (DCs) and activate immune responses that determine disease outcomes.
30429436	3	41	theme	heat-inactivated	478:493	arg1	cells					506:510	heat-inactivated (HI) yeast cells	478:510	heat-inactivated (HI) yeast cells	478:510	The effects of heat-inactivated (HI) yeast cells and soluble mannan of Candida albicans, Candida parapsilosis, and Candida dubliniensis on bone marrow-derived DC (BMDC) responses were compared.
30429436	7	42	theme	proinflammatory	1158:1172	arg1	cytokines					1174:1182	several proinflammatory cytokines	1150:1182	several proinflammatory cytokines which possibly promote hyperinflammation	1150:1223	In contrast, C. parapsilosis mannan caused strong BMDC activation and high production of several proinflammatory cytokines which possibly promote hyperinflammation.
30429436	1	43	theme	first	222:226	arg1	molecules					228:236	the first molecules	218:236	the first molecules that interact with host dendritic cells (DCs) and activate immune responses that determine disease outcomes	218:344	Mannan (mannosylated glycoproteins) in the outermost layer of the Candida cell wall may be the first molecules that interact with host dendritic cells (DCs) and activate immune responses that determine disease outcomes.
30429436	1	43	theme	first	222:226	arg1	Mannan					127:132	Mannan	127:132	Mannan (mannosylated glycoproteins) in the outermost layer of the Candida cell wall	127:209	Mannan (mannosylated glycoproteins) in the outermost layer of the Candida cell wall may be the first molecules that interact with host dendritic cells (DCs) and activate immune responses that determine disease outcomes.
30429436	4	44	theme	yeast	806:810	arg1	wall					817:820	the yeast cell wall	802:820	the yeast cell wall	802:820	HI Candida and the mannan exhibited different effects on BMDC activation and functions, which could be due to other carbohydrate compositions in the yeast cell wall.
30429436	6	45	theme	T	1004:1004	arg1	Th					1014:1015	Th	1014:1015	Th	1014:1015	This suggests the possibility that C. albicans mannan may skew T helper (Th) responses from protective Th17 toward Th1.
30429436	6	45	theme	T	1004:1004	arg1	helper					1006:1011	T helper	1004:1011	T helper (Th) responses	1004:1026	This suggests the possibility that C. albicans mannan may skew T helper (Th) responses from protective Th17 toward Th1.
30429436	3	46	theme	yeast	500:504	arg1	cells					506:510	heat-inactivated (HI) yeast cells	478:510	heat-inactivated (HI) yeast cells	478:510	The effects of heat-inactivated (HI) yeast cells and soluble mannan of Candida albicans, Candida parapsilosis, and Candida dubliniensis on bone marrow-derived DC (BMDC) responses were compared.
30429436	9	47	theme	species	1377:1383	arg1	mannan					1354:1359	mannan	1354:1359	mannan of each Candida species	1354:1383	Therefore, mannan of each Candida species play distinct roles in DC responses and may be involved in the immunopathogenesis and disease severity of oral candidiasis as well as other Candida infection.
30429436	9	48	theme	each	1364:1367	arg1	species					1377:1383	each Candida species	1364:1383	each Candida species	1364:1383	Therefore, mannan of each Candida species play distinct roles in DC responses and may be involved in the immunopathogenesis and disease severity of oral candidiasis as well as other Candida infection.
30429436	4	49	theme	carbohydrate	773:784	arg1	compositions					786:797	other carbohydrate compositions	767:797	other carbohydrate compositions in the yeast cell wall	767:820	HI Candida and the mannan exhibited different effects on BMDC activation and functions, which could be due to other carbohydrate compositions in the yeast cell wall.
30429436	1	50	theme	outermost	170:178	arg1	layer					180:184	the outermost layer	166:184	the outermost layer of the Candida cell wall	166:209	Mannan (mannosylated glycoproteins) in the outermost layer of the Candida cell wall may be the first molecules that interact with host dendritic cells (DCs) and activate immune responses that determine disease outcomes.
30429436	0	51	theme	dubliniensis	113:124	arg1	mannan					51:56	cell wall mannan	41:56	cell wall mannan of Candida albicans, Candida parapsilosis, and Candida dubliniensis	41:124	Differential dendritic cell responses to cell wall mannan of Candida albicans, Candida parapsilosis, and Candida dubliniensis.
30429436	8	52	dep	C.	1237:1238	arg1	dubliniensis					1240:1251	dubliniensis	1240:1251	dubliniensis	1240:1251	Meanwhile, C. dubliniensis mannan induced moderate BMDC responses, which may correlate with its lower pathogenicity.
30429436	9	53	theme	distinct	1390:1397	arg1	roles					1399:1403	distinct roles	1390:1403	distinct roles	1390:1403	Therefore, mannan of each Candida species play distinct roles in DC responses and may be involved in the immunopathogenesis and disease severity of oral candidiasis as well as other Candida infection.
30429436	1	54	theme	Candida	193:199	arg1	wall					206:209	the Candida cell wall	189:209	the Candida cell wall	189:209	Mannan (mannosylated glycoproteins) in the outermost layer of the Candida cell wall may be the first molecules that interact with host dendritic cells (DCs) and activate immune responses that determine disease outcomes.
30429436	1	55	gly	mannosylated	135:146	arg1	Mannan					127:132	Mannan	127:132	Mannan (mannosylated glycoproteins) in the outermost layer of the Candida cell wall	127:209	Mannan (mannosylated glycoproteins) in the outermost layer of the Candida cell wall may be the first molecules that interact with host dendritic cells (DCs) and activate immune responses that determine disease outcomes.
30429436	1	55	gly	mannosylated	135:146	arg1	glycoproteins					148:160	mannosylated glycoproteins	135:160	mannosylated glycoproteins	135:160	Mannan (mannosylated glycoproteins) in the outermost layer of the Candida cell wall may be the first molecules that interact with host dendritic cells (DCs) and activate immune responses that determine disease outcomes.
30429436	3	56	theme	Candida	534:540	arg1	albicans					542:549	Candida albicans	534:549	Candida albicans	534:549	The effects of heat-inactivated (HI) yeast cells and soluble mannan of Candida albicans, Candida parapsilosis, and Candida dubliniensis on bone marrow-derived DC (BMDC) responses were compared.
30429436	4	57	theme	HI	657:658	arg1	Candida					660:666	HI Candida	657:666	HI Candida	657:666	HI Candida and the mannan exhibited different effects on BMDC activation and functions, which could be due to other carbohydrate compositions in the yeast cell wall.
30429436	4	58	theme	BMDC	714:717	arg1	activation					719:728	BMDC activation	714:728	BMDC activation	714:728	HI Candida and the mannan exhibited different effects on BMDC activation and functions, which could be due to other carbohydrate compositions in the yeast cell wall.
30429436	3	59	from	mannan	524:529	arg1	responses					632:640	bone marrow-derived DC (BMDC) responses	602:640	bone marrow-derived DC (BMDC) responses	602:640	The effects of heat-inactivated (HI) yeast cells and soluble mannan of Candida albicans, Candida parapsilosis, and Candida dubliniensis on bone marrow-derived DC (BMDC) responses were compared.
30429436	0	60	theme	Candida	61:67	arg1	albicans					69:76	Candida albicans	61:76	Candida albicans	61:76	Differential dendritic cell responses to cell wall mannan of Candida albicans, Candida parapsilosis, and Candida dubliniensis.
30429436	3	61	theme	parapsilosis	560:571	arg1	mannan					524:529	soluble mannan	516:529	soluble mannan	516:529	The effects of heat-inactivated (HI) yeast cells and soluble mannan of Candida albicans, Candida parapsilosis, and Candida dubliniensis on bone marrow-derived DC (BMDC) responses were compared.
30429436	3	61	theme	parapsilosis	560:571	arg1	effects					467:473	The effects	463:473	The effects of heat-inactivated (HI) yeast cells	463:510	The effects of heat-inactivated (HI) yeast cells and soluble mannan of Candida albicans, Candida parapsilosis, and Candida dubliniensis on bone marrow-derived DC (BMDC) responses were compared.
30429436	8	62	theme	C.	1237:1238	arg1	Meanwhile					1226:1234	Meanwhile	1226:1234	Meanwhile	1226:1234	Meanwhile, C. dubliniensis mannan induced moderate BMDC responses, which may correlate with its lower pathogenicity.
30429436	8	62	theme	C.	1237:1238	arg1	mannan					1253:1258	C. dubliniensis mannan	1237:1258	C. dubliniensis mannan	1237:1258	Meanwhile, C. dubliniensis mannan induced moderate BMDC responses, which may correlate with its lower pathogenicity.
30429436	7	63	theme	strong	1104:1109	arg1	activation					1116:1125	strong BMDC activation	1104:1125	strong BMDC activation	1104:1125	In contrast, C. parapsilosis mannan caused strong BMDC activation and high production of several proinflammatory cytokines which possibly promote hyperinflammation.
30429436	7	64	theme	BMDC	1111:1114	arg1	activation					1116:1125	strong BMDC activation	1104:1125	strong BMDC activation	1104:1125	In contrast, C. parapsilosis mannan caused strong BMDC activation and high production of several proinflammatory cytokines which possibly promote hyperinflammation.
30429436	1	65	theme	immune	297:302	arg1	responses					304:312	immune responses	297:312	immune responses that determine disease outcomes	297:344	Mannan (mannosylated glycoproteins) in the outermost layer of the Candida cell wall may be the first molecules that interact with host dendritic cells (DCs) and activate immune responses that determine disease outcomes.
30429436	2	66	theme	DC	448:449	arg1	activation					451:460	DC activation	448:460	DC activation	448:460	However, little is known about how different mannan structures of common oral Candida species affect DC activation.
30429436	0	67	theme	parapsilosis	87:98	arg1	mannan					51:56	cell wall mannan	41:56	cell wall mannan of Candida albicans, Candida parapsilosis, and Candida dubliniensis	41:124	Differential dendritic cell responses to cell wall mannan of Candida albicans, Candida parapsilosis, and Candida dubliniensis.
30429436	8	68	theme	moderate	1268:1275	arg1	responses					1282:1290	moderate BMDC responses	1268:1290	moderate BMDC responses	1268:1290	Meanwhile, C. dubliniensis mannan induced moderate BMDC responses, which may correlate with its lower pathogenicity.
30429436	7	69	theme	parapsilosis	1077:1088	arg1	mannan					1090:1095	C. parapsilosis mannan	1074:1095	C. parapsilosis mannan	1074:1095	In contrast, C. parapsilosis mannan caused strong BMDC activation and high production of several proinflammatory cytokines which possibly promote hyperinflammation.
30429436	1	70	theme	disease	329:335	arg1	outcomes					337:344	disease outcomes	329:344	disease outcomes	329:344	Mannan (mannosylated glycoproteins) in the outermost layer of the Candida cell wall may be the first molecules that interact with host dendritic cells (DCs) and activate immune responses that determine disease outcomes.
30429436	5	71	dep	C.	849:850	arg1	albicans					852:859	albicans	852:859	albicans	852:859	Among Candida mannan, the C. albicans mannan was the weakest stimulus and induced only interferon (IFN)-γ production.
30429436	0	72	theme	dendritic	13:21	arg1	responses					28:36	Differential dendritic cell responses	0:36	Differential dendritic cell responses to cell wall mannan of Candida albicans, Candida parapsilosis, and Candida dubliniensis	0:124	Differential dendritic cell responses to cell wall mannan of Candida albicans, Candida parapsilosis, and Candida dubliniensis.
30429436	1	73	from	Mannan	127:132	arg1	layer					180:184	the outermost layer	166:184	the outermost layer of the Candida cell wall	166:209	Mannan (mannosylated glycoproteins) in the outermost layer of the Candida cell wall may be the first molecules that interact with host dendritic cells (DCs) and activate immune responses that determine disease outcomes.
30429436	9	74	theme	oral	1491:1494	arg1	candidiasis					1496:1506	oral candidiasis	1491:1506	oral candidiasis as well as other Candida infection	1491:1541	Therefore, mannan of each Candida species play distinct roles in DC responses and may be involved in the immunopathogenesis and disease severity of oral candidiasis as well as other Candida infection.
30429436	9	75	dep	immunopathogenesis	1448:1465	arg1	the					1444:1446	the	1444:1446	the	1444:1446	Therefore, mannan of each Candida species play distinct roles in DC responses and may be involved in the immunopathogenesis and disease severity of oral candidiasis as well as other Candida infection.
30429436	1	76	theme	host	257:260	arg1	DCs					279:281	DCs	279:281	DCs	279:281	Mannan (mannosylated glycoproteins) in the outermost layer of the Candida cell wall may be the first molecules that interact with host dendritic cells (DCs) and activate immune responses that determine disease outcomes.
30429436	1	76	theme	host	257:260	arg1	cells					272:276	host dendritic cells	257:276	host dendritic cells (DCs)	257:282	Mannan (mannosylated glycoproteins) in the outermost layer of the Candida cell wall may be the first molecules that interact with host dendritic cells (DCs) and activate immune responses that determine disease outcomes.
30429436	3	77	dep	heat-inactivated	478:493	arg1	HI					496:497	HI	496:497	HI	496:497	The effects of heat-inactivated (HI) yeast cells and soluble mannan of Candida albicans, Candida parapsilosis, and Candida dubliniensis on bone marrow-derived DC (BMDC) responses were compared.
30429436	6	78	theme	C.	976:977	arg1	mannan					988:993	C. albicans mannan	976:993	C. albicans mannan	976:993	This suggests the possibility that C. albicans mannan may skew T helper (Th) responses from protective Th17 toward Th1.
30429436	2	79	theme	mannan	392:397	arg1	structures					399:408	different mannan structures	382:408	different mannan structures of common oral Candida species	382:439	However, little is known about how different mannan structures of common oral Candida species affect DC activation.
30429436	4	80	theme	different	693:701	arg1	effects					703:709	different effects	693:709	different effects	693:709	HI Candida and the mannan exhibited different effects on BMDC activation and functions, which could be due to other carbohydrate compositions in the yeast cell wall.
30429436	0	81	theme	cell	41:44	arg1	mannan					51:56	cell wall mannan	41:56	cell wall mannan of Candida albicans, Candida parapsilosis, and Candida dubliniensis	41:124	Differential dendritic cell responses to cell wall mannan of Candida albicans, Candida parapsilosis, and Candida dubliniensis.
30429436	8	82	dep	induced	1260:1266	arg1	correlate					1303:1311	correlate	1303:1311	may correlate with its lower pathogenicity	1299:1340	Meanwhile, C. dubliniensis mannan induced moderate BMDC responses, which may correlate with its lower pathogenicity.
30429436	2	83	theme	species	433:439	arg1	structures					399:408	different mannan structures	382:408	different mannan structures of common oral Candida species	382:439	However, little is known about how different mannan structures of common oral Candida species affect DC activation.
30429436	3	84	theme	dubliniensis	586:597	arg1	mannan					524:529	soluble mannan	516:529	soluble mannan	516:529	The effects of heat-inactivated (HI) yeast cells and soluble mannan of Candida albicans, Candida parapsilosis, and Candida dubliniensis on bone marrow-derived DC (BMDC) responses were compared.
30429436	3	84	theme	dubliniensis	586:597	arg1	effects					467:473	The effects	463:473	The effects of heat-inactivated (HI) yeast cells	463:510	The effects of heat-inactivated (HI) yeast cells and soluble mannan of Candida albicans, Candida parapsilosis, and Candida dubliniensis on bone marrow-derived DC (BMDC) responses were compared.
30429436	9	85	theme	Candida	1525:1531	arg1	infection					1533:1541	other Candida infection	1519:1541	oral candidiasis as well as other Candida infection	1491:1541	Therefore, mannan of each Candida species play distinct roles in DC responses and may be involved in the immunopathogenesis and disease severity of oral candidiasis as well as other Candida infection.
30429436	6	86	theme	protective	1033:1042	arg1	Th17					1044:1047	protective Th17	1033:1047	protective Th17 toward Th1	1033:1058	This suggests the possibility that C. albicans mannan may skew T helper (Th) responses from protective Th17 toward Th1.
30429436	2	87	theme	oral	420:423	arg1	species					433:439	common oral Candida species	413:439	common oral Candida species	413:439	However, little is known about how different mannan structures of common oral Candida species affect DC activation.
30429436	7	88	theme	cytokines	1174:1182	arg1	activation					1116:1125	strong BMDC activation	1104:1125	strong BMDC activation	1104:1125	In contrast, C. parapsilosis mannan caused strong BMDC activation and high production of several proinflammatory cytokines which possibly promote hyperinflammation.
30429436	7	88	theme	cytokines	1174:1182	arg1	production					1136:1145	high production	1131:1145	high production of several proinflammatory cytokines which possibly promote hyperinflammation	1131:1223	In contrast, C. parapsilosis mannan caused strong BMDC activation and high production of several proinflammatory cytokines which possibly promote hyperinflammation.
30429436	3	89	theme	DC	622:623	arg1	responses					632:640	bone marrow-derived DC (BMDC) responses	602:640	bone marrow-derived DC (BMDC) responses	602:640	The effects of heat-inactivated (HI) yeast cells and soluble mannan of Candida albicans, Candida parapsilosis, and Candida dubliniensis on bone marrow-derived DC (BMDC) responses were compared.
30429436	9	90	theme	DC	1408:1409	arg1	responses					1411:1419	DC responses	1408:1419	DC responses	1408:1419	Therefore, mannan of each Candida species play distinct roles in DC responses and may be involved in the immunopathogenesis and disease severity of oral candidiasis as well as other Candida infection.
30429436	7	91	theme	several	1150:1156	arg1	cytokines					1174:1182	several proinflammatory cytokines	1150:1182	several proinflammatory cytokines which possibly promote hyperinflammation	1150:1223	In contrast, C. parapsilosis mannan caused strong BMDC activation and high production of several proinflammatory cytokines which possibly promote hyperinflammation.
30247892	8	0	theme	polymer	1338:1344	arg1	spray					1346:1350	the CNT/PMMA conductive polymer spray	1314:1350	the CNT/PMMA conductive polymer spray	1314:1350	These results suggest that the CNT/PMMA conductive polymer spray has great potential in the analysis of hydrophilic drugs, saccharides, peptides, and proteins in biofluids.
30247892	1	1	theme	proteins	232:239	arg1	analysis					174:181	the direct mass spectrometric analysis	144:181	the direct mass spectrometric analysis of hydrophilic drugs, saccharides, peptides, and proteins	144:239	We present a conductive polymer spray ionization (CPSI) method for the direct mass spectrometric analysis of hydrophilic drugs, saccharides, peptides, and proteins in biofluids.
30247892	2	2	theme	wide	421:424	arg1	mm					418:419	8 mm wide	416:424	8 mm wide	416:424	Carbon nanotubes (CNTs) were introduced into poly(methyl methacrylate) (PMMA) to fabricate a conductive composite substrate CNT/PMMA in the shape of a triangle (8 mm wide and 10 mm long) with its apex pointed toward the inlet of a mass spectrometer.
30247892	3	3	theme	hydrophilic	597:607	arg1	compounds					609:617	less hydrophilic compounds	592:617	less hydrophilic compounds owing to its hydrophobic nature	592:649	In comparison with a traditional paper spray substrate, the conductive polymer absorbs less hydrophilic compounds owing to its hydrophobic nature.
30247892	2	4	dep	triangle	406:413	arg1	the					391:393	the	391:393	the	391:393	Carbon nanotubes (CNTs) were introduced into poly(methyl methacrylate) (PMMA) to fabricate a conductive composite substrate CNT/PMMA in the shape of a triangle (8 mm wide and 10 mm long) with its apex pointed toward the inlet of a mass spectrometer.
30247892	2	4	dep	triangle	406:413	arg1	mm					433:434	10 mm long	430:439	10 mm long	430:439	Carbon nanotubes (CNTs) were introduced into poly(methyl methacrylate) (PMMA) to fabricate a conductive composite substrate CNT/PMMA in the shape of a triangle (8 mm wide and 10 mm long) with its apex pointed toward the inlet of a mass spectrometer.
30247892	2	4	dep	triangle	406:413	arg1	shape					395:399	shape	395:399	shape	395:399	Carbon nanotubes (CNTs) were introduced into poly(methyl methacrylate) (PMMA) to fabricate a conductive composite substrate CNT/PMMA in the shape of a triangle (8 mm wide and 10 mm long) with its apex pointed toward the inlet of a mass spectrometer.
30247892	2	4	dep	triangle	406:413	arg1	mm					418:419	8 mm wide	416:424	8 mm wide	416:424	Carbon nanotubes (CNTs) were introduced into poly(methyl methacrylate) (PMMA) to fabricate a conductive composite substrate CNT/PMMA in the shape of a triangle (8 mm wide and 10 mm long) with its apex pointed toward the inlet of a mass spectrometer.
30247892	1	5	theme	direct	148:153	arg1	analysis					174:181	the direct mass spectrometric analysis	144:181	the direct mass spectrometric analysis of hydrophilic drugs, saccharides, peptides, and proteins	144:239	We present a conductive polymer spray ionization (CPSI) method for the direct mass spectrometric analysis of hydrophilic drugs, saccharides, peptides, and proteins in biofluids.
30247892	5	6	theme	applied	893:899	arg1	kV					877:878	4.5 kV high	873:883	4.5 kV high voltage applied	873:899	Only 1.0-2.0 μL solvent suffices to extract the components in a dried biofluid spot and to form charged microdroplets (4.5 kV high voltage applied).
30247892	6	7	theme	typical	1048:1054	arg1	duration					1056:1063	a typical duration	1046:1063	a typical duration of 7.5 min	1046:1074	Furthermore, the hydrophobic polymer surface only needs to overcome weak surface tension to emit charged microdroplets, so that the signal has a typical duration of 7.5 min.
30247892	7	8	theme	compound	1164:1171	arg1	higher					1226:1231	higher	1226:1231	higher	1226:1231	For sunitinib, acarbose, melamine, and angiotensin II, the ion intensity of the target compound from the conductive polymer support is significantly higher than paper spray, typically by a factor of 20 to 100.
30247892	7	8	theme	compound	1164:1171	arg1	intensity					1140:1148	the ion intensity	1132:1148	the ion intensity of the target compound from the conductive polymer support	1132:1207	For sunitinib, acarbose, melamine, and angiotensin II, the ion intensity of the target compound from the conductive polymer support is significantly higher than paper spray, typically by a factor of 20 to 100.
30247892	4	9	theme	biofluid	665:672	arg1	samples					674:680	aqueous biofluid samples	657:680	aqueous biofluid samples	657:680	When aqueous biofluid samples are loaded, they also exhibit less diffusion on this nonporous surface.
30247892	6	10	contain	has	1042:1044	arg2	duration					1056:1063	a typical duration	1046:1063	a typical duration of 7.5 min	1046:1074	Furthermore, the hydrophobic polymer surface only needs to overcome weak surface tension to emit charged microdroplets, so that the signal has a typical duration of 7.5 min.
30247892	6	10	contain	has	1042:1044	arg1	signal					1035:1040	the signal	1031:1040	the signal	1031:1040	Furthermore, the hydrophobic polymer surface only needs to overcome weak surface tension to emit charged microdroplets, so that the signal has a typical duration of 7.5 min.
30247892	3	11	with	comparison	508:517	arg1	substrate					550:558	a traditional paper spray substrate	524:558	a traditional paper spray substrate	524:558	In comparison with a traditional paper spray substrate, the conductive polymer absorbs less hydrophilic compounds owing to its hydrophobic nature.
30247892	2	12	theme	spectrometer	491:502	arg1	inlet					475:479	the inlet	471:479	the inlet of a mass spectrometer	471:502	Carbon nanotubes (CNTs) were introduced into poly(methyl methacrylate) (PMMA) to fabricate a conductive composite substrate CNT/PMMA in the shape of a triangle (8 mm wide and 10 mm long) with its apex pointed toward the inlet of a mass spectrometer.
30247892	1	13	from	method	133:138	arg1	biofluids					244:252	biofluids	244:252	biofluids	244:252	We present a conductive polymer spray ionization (CPSI) method for the direct mass spectrometric analysis of hydrophilic drugs, saccharides, peptides, and proteins in biofluids.
30247892	7	14	theme	100	1282:1284	arg1	factor					1266:1271	a factor	1264:1271	a factor of 20 to 100	1264:1284	For sunitinib, acarbose, melamine, and angiotensin II, the ion intensity of the target compound from the conductive polymer support is significantly higher than paper spray, typically by a factor of 20 to 100.
30247892	7	15	from	support	1201:1207	arg1	higher					1226:1231	higher	1226:1231	higher	1226:1231	For sunitinib, acarbose, melamine, and angiotensin II, the ion intensity of the target compound from the conductive polymer support is significantly higher than paper spray, typically by a factor of 20 to 100.
30247892	7	15	from	support	1201:1207	arg1	intensity					1140:1148	the ion intensity	1132:1148	the ion intensity of the target compound from the conductive polymer support	1132:1207	For sunitinib, acarbose, melamine, and angiotensin II, the ion intensity of the target compound from the conductive polymer support is significantly higher than paper spray, typically by a factor of 20 to 100.
30247892	7	16	theme	target	1157:1162	arg1	compound					1164:1171	the target compound	1153:1171	the target compound	1153:1171	For sunitinib, acarbose, melamine, and angiotensin II, the ion intensity of the target compound from the conductive polymer support is significantly higher than paper spray, typically by a factor of 20 to 100.
30247892	5	17	theme	dried	818:822	arg1	spot					833:836	a dried biofluid spot	816:836	a dried biofluid spot	816:836	Only 1.0-2.0 μL solvent suffices to extract the components in a dried biofluid spot and to form charged microdroplets (4.5 kV high voltage applied).
30247892	5	18	from	components	802:811	arg1	spot					833:836	a dried biofluid spot	816:836	a dried biofluid spot	816:836	Only 1.0-2.0 μL solvent suffices to extract the components in a dried biofluid spot and to form charged microdroplets (4.5 kV high voltage applied).
30247892	6	19	theme	hydrophobic	920:930	arg1	surface					940:946	the hydrophobic polymer surface	916:946	the hydrophobic polymer surface	916:946	Furthermore, the hydrophobic polymer surface only needs to overcome weak surface tension to emit charged microdroplets, so that the signal has a typical duration of 7.5 min.
30247892	1	20	theme	mass	155:158	arg1	analysis					174:181	the direct mass spectrometric analysis	144:181	the direct mass spectrometric analysis of hydrophilic drugs, saccharides, peptides, and proteins	144:239	We present a conductive polymer spray ionization (CPSI) method for the direct mass spectrometric analysis of hydrophilic drugs, saccharides, peptides, and proteins in biofluids.
30247892	2	21	theme	long	436:439	arg1	mm					433:434	10 mm long	430:439	10 mm long	430:439	Carbon nanotubes (CNTs) were introduced into poly(methyl methacrylate) (PMMA) to fabricate a conductive composite substrate CNT/PMMA in the shape of a triangle (8 mm wide and 10 mm long) with its apex pointed toward the inlet of a mass spectrometer.
30247892	2	22	theme	Carbon	255:260	arg1	CNTs					273:276	CNTs	273:276	CNTs	273:276	Carbon nanotubes (CNTs) were introduced into poly(methyl methacrylate) (PMMA) to fabricate a conductive composite substrate CNT/PMMA in the shape of a triangle (8 mm wide and 10 mm long) with its apex pointed toward the inlet of a mass spectrometer.
30247892	2	22	theme	Carbon	255:260	arg1	nanotubes					262:270	Carbon nanotubes	255:270	Carbon nanotubes (CNTs)	255:277	Carbon nanotubes (CNTs) were introduced into poly(methyl methacrylate) (PMMA) to fabricate a conductive composite substrate CNT/PMMA in the shape of a triangle (8 mm wide and 10 mm long) with its apex pointed toward the inlet of a mass spectrometer.
30247892	1	23	theme	spectrometric	160:172	arg1	analysis					174:181	the direct mass spectrometric analysis	144:181	the direct mass spectrometric analysis of hydrophilic drugs, saccharides, peptides, and proteins	144:239	We present a conductive polymer spray ionization (CPSI) method for the direct mass spectrometric analysis of hydrophilic drugs, saccharides, peptides, and proteins in biofluids.
30247892	0	24	theme	Polymer	11:17	arg1	Spectrometry					41:52	Conductive Polymer Spray Ionization Mass Spectrometry	0:52	Conductive Polymer Spray Ionization Mass Spectrometry for Biofluid Analysis	0:74	Conductive Polymer Spray Ionization Mass Spectrometry for Biofluid Analysis.
30247892	3	25	theme	hydrophobic	632:642	arg1	nature					644:649	its hydrophobic nature	628:649	its hydrophobic nature	628:649	In comparison with a traditional paper spray substrate, the conductive polymer absorbs less hydrophilic compounds owing to its hydrophobic nature.
30247892	6	26	theme	surface	976:982	arg1	tension					984:990	weak surface tension	971:990	weak surface tension	971:990	Furthermore, the hydrophobic polymer surface only needs to overcome weak surface tension to emit charged microdroplets, so that the signal has a typical duration of 7.5 min.
30247892	4	27	theme	aqueous	657:663	arg1	samples					674:680	aqueous biofluid samples	657:680	aqueous biofluid samples	657:680	When aqueous biofluid samples are loaded, they also exhibit less diffusion on this nonporous surface.
30247892	0	28	theme	Conductive	0:9	arg1	Spectrometry					41:52	Conductive Polymer Spray Ionization Mass Spectrometry	0:52	Conductive Polymer Spray Ionization Mass Spectrometry for Biofluid Analysis	0:74	Conductive Polymer Spray Ionization Mass Spectrometry for Biofluid Analysis.
30247892	8	29	theme	hydrophilic	1391:1401	arg1	drugs					1403:1407	hydrophilic drugs	1391:1407	hydrophilic drugs	1391:1407	These results suggest that the CNT/PMMA conductive polymer spray has great potential in the analysis of hydrophilic drugs, saccharides, peptides, and proteins in biofluids.
30247892	8	30	theme	CNT/PMMA	1318:1325	arg1	spray					1346:1350	the CNT/PMMA conductive polymer spray	1314:1350	the CNT/PMMA conductive polymer spray	1314:1350	These results suggest that the CNT/PMMA conductive polymer spray has great potential in the analysis of hydrophilic drugs, saccharides, peptides, and proteins in biofluids.
30247892	1	31	theme	conductive	90:99	arg1	method					133:138	a conductive polymer spray ionization (CPSI) method	88:138	a conductive polymer spray ionization (CPSI) method for the direct mass spectrometric analysis of hydrophilic drugs, saccharides, peptides, and proteins in biofluids	88:252	We present a conductive polymer spray ionization (CPSI) method for the direct mass spectrometric analysis of hydrophilic drugs, saccharides, peptides, and proteins in biofluids.
30247892	8	32	contain	has	1352:1354	arg1	spray					1346:1350	the CNT/PMMA conductive polymer spray	1314:1350	the CNT/PMMA conductive polymer spray	1314:1350	These results suggest that the CNT/PMMA conductive polymer spray has great potential in the analysis of hydrophilic drugs, saccharides, peptides, and proteins in biofluids.
30247892	8	32	contain	has	1352:1354	arg2	potential					1362:1370	great potential	1356:1370	great potential	1356:1370	These results suggest that the CNT/PMMA conductive polymer spray has great potential in the analysis of hydrophilic drugs, saccharides, peptides, and proteins in biofluids.
30247892	8	33	theme	conductive	1327:1336	arg1	spray					1346:1350	the CNT/PMMA conductive polymer spray	1314:1350	the CNT/PMMA conductive polymer spray	1314:1350	These results suggest that the CNT/PMMA conductive polymer spray has great potential in the analysis of hydrophilic drugs, saccharides, peptides, and proteins in biofluids.
30247892	0	34	theme	Ionization	25:34	arg1	Spectrometry					41:52	Conductive Polymer Spray Ionization Mass Spectrometry	0:52	Conductive Polymer Spray Ionization Mass Spectrometry for Biofluid Analysis	0:74	Conductive Polymer Spray Ionization Mass Spectrometry for Biofluid Analysis.
30247892	7	35	theme	paper	1238:1242	arg1	spray					1244:1248	paper spray	1238:1248	paper spray	1238:1248	For sunitinib, acarbose, melamine, and angiotensin II, the ion intensity of the target compound from the conductive polymer support is significantly higher than paper spray, typically by a factor of 20 to 100.
30247892	1	36	theme	polymer	101:107	arg1	method					133:138	a conductive polymer spray ionization (CPSI) method	88:138	a conductive polymer spray ionization (CPSI) method for the direct mass spectrometric analysis of hydrophilic drugs, saccharides, peptides, and proteins in biofluids	88:252	We present a conductive polymer spray ionization (CPSI) method for the direct mass spectrometric analysis of hydrophilic drugs, saccharides, peptides, and proteins in biofluids.
30247892	1	37	theme	hydrophilic	186:196	arg1	drugs					198:202	hydrophilic drugs	186:202	hydrophilic drugs	186:202	We present a conductive polymer spray ionization (CPSI) method for the direct mass spectrometric analysis of hydrophilic drugs, saccharides, peptides, and proteins in biofluids.
30247892	0	38	theme	Spray	19:23	arg1	Spectrometry					41:52	Conductive Polymer Spray Ionization Mass Spectrometry	0:52	Conductive Polymer Spray Ionization Mass Spectrometry for Biofluid Analysis	0:74	Conductive Polymer Spray Ionization Mass Spectrometry for Biofluid Analysis.
30247892	1	39	theme	spray	109:113	arg1	method					133:138	a conductive polymer spray ionization (CPSI) method	88:138	a conductive polymer spray ionization (CPSI) method for the direct mass spectrometric analysis of hydrophilic drugs, saccharides, peptides, and proteins in biofluids	88:252	We present a conductive polymer spray ionization (CPSI) method for the direct mass spectrometric analysis of hydrophilic drugs, saccharides, peptides, and proteins in biofluids.
30247892	6	40	theme	polymer	932:938	arg1	surface					940:946	the hydrophobic polymer surface	916:946	the hydrophobic polymer surface	916:946	Furthermore, the hydrophobic polymer surface only needs to overcome weak surface tension to emit charged microdroplets, so that the signal has a typical duration of 7.5 min.
30247892	1	41	theme	drugs	198:202	arg1	analysis					174:181	the direct mass spectrometric analysis	144:181	the direct mass spectrometric analysis of hydrophilic drugs, saccharides, peptides, and proteins	144:239	We present a conductive polymer spray ionization (CPSI) method for the direct mass spectrometric analysis of hydrophilic drugs, saccharides, peptides, and proteins in biofluids.
30247892	8	42	from	analysis	1379:1386	arg1	biofluids					1449:1457	biofluids	1449:1457	biofluids	1449:1457	These results suggest that the CNT/PMMA conductive polymer spray has great potential in the analysis of hydrophilic drugs, saccharides, peptides, and proteins in biofluids.
30247892	5	43	theme	biofluid	824:831	arg1	spot					833:836	a dried biofluid spot	816:836	a dried biofluid spot	816:836	Only 1.0-2.0 μL solvent suffices to extract the components in a dried biofluid spot and to form charged microdroplets (4.5 kV high voltage applied).
30247892	2	44	theme	substrate	369:377	arg1	CNT/PMMA					379:386	a conductive composite substrate CNT/PMMA	346:386	a conductive composite substrate CNT/PMMA	346:386	Carbon nanotubes (CNTs) were introduced into poly(methyl methacrylate) (PMMA) to fabricate a conductive composite substrate CNT/PMMA in the shape of a triangle (8 mm wide and 10 mm long) with its apex pointed toward the inlet of a mass spectrometer.
30247892	0	45	theme	Mass	36:39	arg1	Spectrometry					41:52	Conductive Polymer Spray Ionization Mass Spectrometry	0:52	Conductive Polymer Spray Ionization Mass Spectrometry for Biofluid Analysis	0:74	Conductive Polymer Spray Ionization Mass Spectrometry for Biofluid Analysis.
30247892	3	46	theme	spray	544:548	arg1	substrate					550:558	a traditional paper spray substrate	524:558	a traditional paper spray substrate	524:558	In comparison with a traditional paper spray substrate, the conductive polymer absorbs less hydrophilic compounds owing to its hydrophobic nature.
30247892	5	47	theme	μL	767:768	arg1	solvent					770:776	Only 1.0-2.0 μL solvent	754:776	Only 1.0-2.0 μL solvent	754:776	Only 1.0-2.0 μL solvent suffices to extract the components in a dried biofluid spot and to form charged microdroplets (4.5 kV high voltage applied).
30247892	1	48	theme	saccharides	205:215	arg1	analysis					174:181	the direct mass spectrometric analysis	144:181	the direct mass spectrometric analysis of hydrophilic drugs, saccharides, peptides, and proteins	144:239	We present a conductive polymer spray ionization (CPSI) method for the direct mass spectrometric analysis of hydrophilic drugs, saccharides, peptides, and proteins in biofluids.
30247892	0	49	theme	Biofluid	58:65	arg1	Analysis					67:74	Biofluid Analysis	58:74	Biofluid Analysis	58:74	Conductive Polymer Spray Ionization Mass Spectrometry for Biofluid Analysis.
30247892	8	50	theme	saccharides	1410:1420	arg1	analysis					1379:1386	the analysis	1375:1386	the analysis of hydrophilic drugs, saccharides, peptides, and proteins in biofluids	1375:1457	These results suggest that the CNT/PMMA conductive polymer spray has great potential in the analysis of hydrophilic drugs, saccharides, peptides, and proteins in biofluids.
30247892	2	51	theme	mass	486:489	arg1	spectrometer					491:502	a mass spectrometer	484:502	a mass spectrometer	484:502	Carbon nanotubes (CNTs) were introduced into poly(methyl methacrylate) (PMMA) to fabricate a conductive composite substrate CNT/PMMA in the shape of a triangle (8 mm wide and 10 mm long) with its apex pointed toward the inlet of a mass spectrometer.
30247892	1	52	theme	ionization	115:124	arg1	method					133:138	a conductive polymer spray ionization (CPSI) method	88:138	a conductive polymer spray ionization (CPSI) method for the direct mass spectrometric analysis of hydrophilic drugs, saccharides, peptides, and proteins in biofluids	88:252	We present a conductive polymer spray ionization (CPSI) method for the direct mass spectrometric analysis of hydrophilic drugs, saccharides, peptides, and proteins in biofluids.
30247892	2	53	theme	conductive	348:357	arg1	CNT/PMMA					379:386	a conductive composite substrate CNT/PMMA	346:386	a conductive composite substrate CNT/PMMA	346:386	Carbon nanotubes (CNTs) were introduced into poly(methyl methacrylate) (PMMA) to fabricate a conductive composite substrate CNT/PMMA in the shape of a triangle (8 mm wide and 10 mm long) with its apex pointed toward the inlet of a mass spectrometer.
30247892	8	54	theme	drugs	1403:1407	arg1	analysis					1379:1386	the analysis	1375:1386	the analysis of hydrophilic drugs, saccharides, peptides, and proteins in biofluids	1375:1457	These results suggest that the CNT/PMMA conductive polymer spray has great potential in the analysis of hydrophilic drugs, saccharides, peptides, and proteins in biofluids.
30247892	4	55	theme	nonporous	735:743	arg1	surface					745:751	this nonporous surface	730:751	this nonporous surface	730:751	When aqueous biofluid samples are loaded, they also exhibit less diffusion on this nonporous surface.
30247892	7	56	theme	ion	1136:1138	arg1	higher					1226:1231	higher	1226:1231	higher	1226:1231	For sunitinib, acarbose, melamine, and angiotensin II, the ion intensity of the target compound from the conductive polymer support is significantly higher than paper spray, typically by a factor of 20 to 100.
30247892	7	56	theme	ion	1136:1138	arg1	intensity					1140:1148	the ion intensity	1132:1148	the ion intensity of the target compound from the conductive polymer support	1132:1207	For sunitinib, acarbose, melamine, and angiotensin II, the ion intensity of the target compound from the conductive polymer support is significantly higher than paper spray, typically by a factor of 20 to 100.
30247892	2	57	theme	methyl	305:310	arg1	poly					300:303	poly	300:303	poly(methyl methacrylate) (PMMA)	300:331	Carbon nanotubes (CNTs) were introduced into poly(methyl methacrylate) (PMMA) to fabricate a conductive composite substrate CNT/PMMA in the shape of a triangle (8 mm wide and 10 mm long) with its apex pointed toward the inlet of a mass spectrometer.
30247892	2	57	theme	methyl	305:310	arg1	methacrylate					312:323	methyl methacrylate	305:323	methyl methacrylate	305:323	Carbon nanotubes (CNTs) were introduced into poly(methyl methacrylate) (PMMA) to fabricate a conductive composite substrate CNT/PMMA in the shape of a triangle (8 mm wide and 10 mm long) with its apex pointed toward the inlet of a mass spectrometer.
30247892	8	58	theme	peptides	1423:1430	arg1	analysis					1379:1386	the analysis	1375:1386	the analysis of hydrophilic drugs, saccharides, peptides, and proteins in biofluids	1375:1457	These results suggest that the CNT/PMMA conductive polymer spray has great potential in the analysis of hydrophilic drugs, saccharides, peptides, and proteins in biofluids.
30247892	7	59	theme	conductive	1182:1191	arg1	support					1201:1207	the conductive polymer support	1178:1207	the conductive polymer support	1178:1207	For sunitinib, acarbose, melamine, and angiotensin II, the ion intensity of the target compound from the conductive polymer support is significantly higher than paper spray, typically by a factor of 20 to 100.
30247892	3	60	theme	traditional	526:536	arg1	substrate					550:558	a traditional paper spray substrate	524:558	a traditional paper spray substrate	524:558	In comparison with a traditional paper spray substrate, the conductive polymer absorbs less hydrophilic compounds owing to its hydrophobic nature.
30247892	7	61	theme	polymer	1193:1199	arg1	support					1201:1207	the conductive polymer support	1178:1207	the conductive polymer support	1178:1207	For sunitinib, acarbose, melamine, and angiotensin II, the ion intensity of the target compound from the conductive polymer support is significantly higher than paper spray, typically by a factor of 20 to 100.
30247892	1	62	theme	CPSI	127:130	arg1	method					133:138	a conductive polymer spray ionization (CPSI) method	88:138	a conductive polymer spray ionization (CPSI) method for the direct mass spectrometric analysis of hydrophilic drugs, saccharides, peptides, and proteins in biofluids	88:252	We present a conductive polymer spray ionization (CPSI) method for the direct mass spectrometric analysis of hydrophilic drugs, saccharides, peptides, and proteins in biofluids.
30247892	5	63	theme	charged	850:856	arg1	microdroplets					858:870	charged microdroplets	850:870	charged microdroplets (4.5 kV high voltage applied)	850:900	Only 1.0-2.0 μL solvent suffices to extract the components in a dried biofluid spot and to form charged microdroplets (4.5 kV high voltage applied).
30247892	5	64	dep	applied	893:899	arg1	voltage					885:891	voltage	885:891	voltage	885:891	Only 1.0-2.0 μL solvent suffices to extract the components in a dried biofluid spot and to form charged microdroplets (4.5 kV high voltage applied).
30247892	5	65	theme	high	880:883	arg1	kV					877:878	4.5 kV high	873:883	4.5 kV high voltage applied	873:899	Only 1.0-2.0 μL solvent suffices to extract the components in a dried biofluid spot and to form charged microdroplets (4.5 kV high voltage applied).
30247892	3	66	theme	conductive	565:574	arg1	polymer					576:582	the conductive polymer	561:582	the conductive polymer	561:582	In comparison with a traditional paper spray substrate, the conductive polymer absorbs less hydrophilic compounds owing to its hydrophobic nature.
30247892	3	67	theme	paper	538:542	arg1	substrate					550:558	a traditional paper spray substrate	524:558	a traditional paper spray substrate	524:558	In comparison with a traditional paper spray substrate, the conductive polymer absorbs less hydrophilic compounds owing to its hydrophobic nature.
30247892	7	68	dep	100	1282:1284	arg1	to					1279:1280	to	1279:1280	to	1279:1280	For sunitinib, acarbose, melamine, and angiotensin II, the ion intensity of the target compound from the conductive polymer support is significantly higher than paper spray, typically by a factor of 20 to 100.
30247892	1	69	theme	peptides	218:225	arg1	analysis					174:181	the direct mass spectrometric analysis	144:181	the direct mass spectrometric analysis of hydrophilic drugs, saccharides, peptides, and proteins	144:239	We present a conductive polymer spray ionization (CPSI) method for the direct mass spectrometric analysis of hydrophilic drugs, saccharides, peptides, and proteins in biofluids.
30247892	8	70	theme	great	1356:1360	arg1	potential					1362:1370	great potential	1356:1370	great potential	1356:1370	These results suggest that the CNT/PMMA conductive polymer spray has great potential in the analysis of hydrophilic drugs, saccharides, peptides, and proteins in biofluids.
30247892	2	71	theme	composite	359:367	arg1	CNT/PMMA					379:386	a conductive composite substrate CNT/PMMA	346:386	a conductive composite substrate CNT/PMMA	346:386	Carbon nanotubes (CNTs) were introduced into poly(methyl methacrylate) (PMMA) to fabricate a conductive composite substrate CNT/PMMA in the shape of a triangle (8 mm wide and 10 mm long) with its apex pointed toward the inlet of a mass spectrometer.
30247892	2	72	with	triangle	406:413	arg1	apex					451:454	its apex	447:454	its apex pointed toward the inlet of a mass spectrometer	447:502	Carbon nanotubes (CNTs) were introduced into poly(methyl methacrylate) (PMMA) to fabricate a conductive composite substrate CNT/PMMA in the shape of a triangle (8 mm wide and 10 mm long) with its apex pointed toward the inlet of a mass spectrometer.
30247892	6	73	theme	charged	1000:1006	arg1	microdroplets					1008:1020	charged microdroplets	1000:1020	charged microdroplets	1000:1020	Furthermore, the hydrophobic polymer surface only needs to overcome weak surface tension to emit charged microdroplets, so that the signal has a typical duration of 7.5 min.
30247892	6	74	theme	weak	971:974	arg1	tension					984:990	weak surface tension	971:990	weak surface tension	971:990	Furthermore, the hydrophobic polymer surface only needs to overcome weak surface tension to emit charged microdroplets, so that the signal has a typical duration of 7.5 min.
30247892	8	75	theme	proteins	1437:1444	arg1	analysis					1379:1386	the analysis	1375:1386	the analysis of hydrophilic drugs, saccharides, peptides, and proteins in biofluids	1375:1457	These results suggest that the CNT/PMMA conductive polymer spray has great potential in the analysis of hydrophilic drugs, saccharides, peptides, and proteins in biofluids.
30247892	6	76	theme	min	1072:1074	arg1	duration					1056:1063	a typical duration	1046:1063	a typical duration of 7.5 min	1046:1074	Furthermore, the hydrophobic polymer surface only needs to overcome weak surface tension to emit charged microdroplets, so that the signal has a typical duration of 7.5 min.
30247892	5	77	dep	microdroplets	858:870	arg1	kV					877:878	4.5 kV high	873:883	4.5 kV high voltage applied	873:899	Only 1.0-2.0 μL solvent suffices to extract the components in a dried biofluid spot and to form charged microdroplets (4.5 kV high voltage applied).
30914498	3	0	theme	Zea	734:736	arg1	maize					727:731	maize	727:731	maize (Zea mays)	727:742	However, immunosorbence-based screens and carbohydrate linkage analysis of polysaccharides in Golgi membranes, enriched by flotation centrifugation from etiolated coleoptiles of maize (Zea mays) and leaves of Arabidopsis (Arabidopsis thaliana), showed that arabinogalactan-proteins and arabinans represent substantial portions of the Golgi-resident polysaccharides not typically found in high abundance in cell walls of either species.
30914498	3	0	theme	Zea	734:736	arg1	mays					738:741	Zea mays	734:741	Zea mays	734:741	However, immunosorbence-based screens and carbohydrate linkage analysis of polysaccharides in Golgi membranes, enriched by flotation centrifugation from etiolated coleoptiles of maize (Zea mays) and leaves of Arabidopsis (Arabidopsis thaliana), showed that arabinogalactan-proteins and arabinans represent substantial portions of the Golgi-resident polysaccharides not typically found in high abundance in cell walls of either species.
30914498	5	1	theme	cell-wall	1485:1493	arg1	type					1495:1498	cell-wall type	1485:1498	cell-wall type	1485:1498	Consistent with this finding, maize Golgi membranes isolated by flotation centrifugation and enriched further by free-flow electrophoresis, yielded >200 proteins known to function in the biosynthesis and metabolism of cell-wall polysaccharides common to all angiosperms, and not just those specific to cell-wall type.
30914498	6	2	theme	synthase	1797:1804	arg1	complexes					1806:1814	specific synthase complexes	1788:1814	specific synthase complexes	1788:1814	We propose that the distinctive compositions of grass primary cell walls compared with other angiosperms result from differential gating or metabolism of secreted polysaccharides post-Golgi by an as-yet unknown mechanism, and not necessarily by differential expression of genes encoding specific synthase complexes.
30914498	2	3	theme	distinct	349:356	arg1	walls					343:347	cell walls	338:347	cell walls distinct from those of dicots and noncommelinid monocots	338:404	As grass species make cell walls distinct from those of dicots and noncommelinid monocots, it has been assumed that the differences in cell-wall composition stem from differences in biosynthetic capacities of their respective Golgi.
30914498	3	4	theme	high	937:940	arg1	abundance					942:950	high abundance	937:950	high abundance in cell walls of either species	937:982	However, immunosorbence-based screens and carbohydrate linkage analysis of polysaccharides in Golgi membranes, enriched by flotation centrifugation from etiolated coleoptiles of maize (Zea mays) and leaves of Arabidopsis (Arabidopsis thaliana), showed that arabinogalactan-proteins and arabinans represent substantial portions of the Golgi-resident polysaccharides not typically found in high abundance in cell walls of either species.
30914498	5	5	dep	membranes	1225:1233	arg1	enriched					1276:1283	enriched	1276:1283	enriched further by free-flow electrophoresis	1276:1320	Consistent with this finding, maize Golgi membranes isolated by flotation centrifugation and enriched further by free-flow electrophoresis, yielded >200 proteins known to function in the biosynthesis and metabolism of cell-wall polysaccharides common to all angiosperms, and not just those specific to cell-wall type.
30914498	5	5	dep	membranes	1225:1233	arg1	isolated					1235:1242	isolated	1235:1242	isolated by flotation centrifugation	1235:1270	Consistent with this finding, maize Golgi membranes isolated by flotation centrifugation and enriched further by free-flow electrophoresis, yielded >200 proteins known to function in the biosynthesis and metabolism of cell-wall polysaccharides common to all angiosperms, and not just those specific to cell-wall type.
30914498	6	6	theme	as-yet	1697:1702	arg1	mechanism					1712:1720	an as-yet unknown mechanism	1694:1720	an as-yet unknown mechanism	1694:1720	We propose that the distinctive compositions of grass primary cell walls compared with other angiosperms result from differential gating or metabolism of secreted polysaccharides post-Golgi by an as-yet unknown mechanism, and not necessarily by differential expression of genes encoding specific synthase complexes.
30914498	0	7	with	Angiosperms	74:84	arg1	Structures					110:119	Distinct Cell-Wall Structures	91:119	Distinct Cell-Wall Structures	91:119	Glycome and Proteome Components of Golgi Membranes Are Common between Two Angiosperms with Distinct Cell-Wall Structures.
30914498	1	8	theme	proteoglycans	260:272	arg1	synthesis					185:193	synthesis	185:193	synthesis	185:193	The plant endoplasmic reticulum-Golgi apparatus is the site of synthesis, assembly, and trafficking of all noncellulosic polysaccharides, proteoglycans, and proteins destined for the cell wall.
30914498	1	8	theme	proteoglycans	260:272	arg1	assembly					196:203	assembly	196:203	assembly	196:203	The plant endoplasmic reticulum-Golgi apparatus is the site of synthesis, assembly, and trafficking of all noncellulosic polysaccharides, proteoglycans, and proteins destined for the cell wall.
30914498	1	8	theme	proteoglycans	260:272	arg1	trafficking					210:220	trafficking	210:220	trafficking	210:220	The plant endoplasmic reticulum-Golgi apparatus is the site of synthesis, assembly, and trafficking of all noncellulosic polysaccharides, proteoglycans, and proteins destined for the cell wall.
30914498	0	9	theme	Distinct	91:98	arg1	Structures					110:119	Distinct Cell-Wall Structures	91:119	Distinct Cell-Wall Structures	91:119	Glycome and Proteome Components of Golgi Membranes Are Common between Two Angiosperms with Distinct Cell-Wall Structures.
30914498	3	10	theme	maize	727:731	arg1	coleoptiles					712:722	etiolated coleoptiles	702:722	etiolated coleoptiles of maize (Zea mays)	702:742	However, immunosorbence-based screens and carbohydrate linkage analysis of polysaccharides in Golgi membranes, enriched by flotation centrifugation from etiolated coleoptiles of maize (Zea mays) and leaves of Arabidopsis (Arabidopsis thaliana), showed that arabinogalactan-proteins and arabinans represent substantial portions of the Golgi-resident polysaccharides not typically found in high abundance in cell walls of either species.
30914498	3	11	theme	carbohydrate	591:602	arg1	analysis					612:619	carbohydrate linkage analysis	591:619	carbohydrate linkage analysis of polysaccharides in Golgi membranes, enriched by flotation centrifugation from etiolated coleoptiles of maize (Zea mays) and leaves of Arabidopsis (Arabidopsis thaliana),	591:792	However, immunosorbence-based screens and carbohydrate linkage analysis of polysaccharides in Golgi membranes, enriched by flotation centrifugation from etiolated coleoptiles of maize (Zea mays) and leaves of Arabidopsis (Arabidopsis thaliana), showed that arabinogalactan-proteins and arabinans represent substantial portions of the Golgi-resident polysaccharides not typically found in high abundance in cell walls of either species.
30914498	6	12	theme	other	1588:1592	arg1	angiosperms					1594:1604	other angiosperms	1588:1604	other angiosperms	1588:1604	We propose that the distinctive compositions of grass primary cell walls compared with other angiosperms result from differential gating or metabolism of secreted polysaccharides post-Golgi by an as-yet unknown mechanism, and not necessarily by differential expression of genes encoding specific synthase complexes.
30914498	5	13	theme	flotation	1247:1255	arg1	centrifugation					1257:1270	flotation centrifugation	1247:1270	flotation centrifugation	1247:1270	Consistent with this finding, maize Golgi membranes isolated by flotation centrifugation and enriched further by free-flow electrophoresis, yielded >200 proteins known to function in the biosynthesis and metabolism of cell-wall polysaccharides common to all angiosperms, and not just those specific to cell-wall type.
30914498	5	14	theme	polysaccharides	1411:1425	arg1	metabolism					1387:1396	metabolism	1387:1396	metabolism	1387:1396	Consistent with this finding, maize Golgi membranes isolated by flotation centrifugation and enriched further by free-flow electrophoresis, yielded >200 proteins known to function in the biosynthesis and metabolism of cell-wall polysaccharides common to all angiosperms, and not just those specific to cell-wall type.
30914498	5	14	theme	polysaccharides	1411:1425	arg1	biosynthesis					1370:1381	biosynthesis	1370:1381	biosynthesis	1370:1381	Consistent with this finding, maize Golgi membranes isolated by flotation centrifugation and enriched further by free-flow electrophoresis, yielded >200 proteins known to function in the biosynthesis and metabolism of cell-wall polysaccharides common to all angiosperms, and not just those specific to cell-wall type.
30914498	6	15	theme	polysaccharides	1664:1678	arg1	metabolism					1641:1650	metabolism	1641:1650	metabolism	1641:1650	We propose that the distinctive compositions of grass primary cell walls compared with other angiosperms result from differential gating or metabolism of secreted polysaccharides post-Golgi by an as-yet unknown mechanism, and not necessarily by differential expression of genes encoding specific synthase complexes.
30914498	6	15	theme	polysaccharides	1664:1678	arg1	gating					1631:1636	differential gating	1618:1636	differential gating	1618:1636	We propose that the distinctive compositions of grass primary cell walls compared with other angiosperms result from differential gating or metabolism of secreted polysaccharides post-Golgi by an as-yet unknown mechanism, and not necessarily by differential expression of genes encoding specific synthase complexes.
30914498	5	16	with	Consistent	1183:1192	arg1	finding					1204:1210	this finding	1199:1210	this finding	1199:1210	Consistent with this finding, maize Golgi membranes isolated by flotation centrifugation and enriched further by free-flow electrophoresis, yielded >200 proteins known to function in the biosynthesis and metabolism of cell-wall polysaccharides common to all angiosperms, and not just those specific to cell-wall type.
30914498	6	17	theme	walls	1568:1572	arg1	compositions					1533:1544	the distinctive compositions	1517:1544	the distinctive compositions of grass primary cell walls compared with other angiosperms	1517:1604	We propose that the distinctive compositions of grass primary cell walls compared with other angiosperms result from differential gating or metabolism of secreted polysaccharides post-Golgi by an as-yet unknown mechanism, and not necessarily by differential expression of genes encoding specific synthase complexes.
30914498	3	18	theme	cell	955:958	arg1	walls					960:964	cell walls	955:964	cell walls of either species	955:982	However, immunosorbence-based screens and carbohydrate linkage analysis of polysaccharides in Golgi membranes, enriched by flotation centrifugation from etiolated coleoptiles of maize (Zea mays) and leaves of Arabidopsis (Arabidopsis thaliana), showed that arabinogalactan-proteins and arabinans represent substantial portions of the Golgi-resident polysaccharides not typically found in high abundance in cell walls of either species.
30914498	1	19	theme	endoplasmic	132:142	arg1	synthesis					185:193	synthesis	185:193	synthesis	185:193	The plant endoplasmic reticulum-Golgi apparatus is the site of synthesis, assembly, and trafficking of all noncellulosic polysaccharides, proteoglycans, and proteins destined for the cell wall.
30914498	1	19	theme	endoplasmic	132:142	arg1	apparatus					160:168	The plant endoplasmic reticulum-Golgi apparatus	122:168	The plant endoplasmic reticulum-Golgi apparatus	122:168	The plant endoplasmic reticulum-Golgi apparatus is the site of synthesis, assembly, and trafficking of all noncellulosic polysaccharides, proteoglycans, and proteins destined for the cell wall.
30914498	1	19	theme	endoplasmic	132:142	arg1	site					177:180	the site	173:180	the site of synthesis, assembly, and trafficking of all noncellulosic polysaccharides, proteoglycans, and proteins destined for the cell wall	173:313	The plant endoplasmic reticulum-Golgi apparatus is the site of synthesis, assembly, and trafficking of all noncellulosic polysaccharides, proteoglycans, and proteins destined for the cell wall.
30914498	1	19	theme	endoplasmic	132:142	arg1	assembly					196:203	assembly	196:203	assembly	196:203	The plant endoplasmic reticulum-Golgi apparatus is the site of synthesis, assembly, and trafficking of all noncellulosic polysaccharides, proteoglycans, and proteins destined for the cell wall.
30914498	1	19	theme	endoplasmic	132:142	arg1	trafficking					210:220	trafficking	210:220	trafficking	210:220	The plant endoplasmic reticulum-Golgi apparatus is the site of synthesis, assembly, and trafficking of all noncellulosic polysaccharides, proteoglycans, and proteins destined for the cell wall.
30914498	2	20	from	differences	436:446	arg1	composition					461:471	cell-wall composition	451:471	cell-wall composition	451:471	As grass species make cell walls distinct from those of dicots and noncommelinid monocots, it has been assumed that the differences in cell-wall composition stem from differences in biosynthetic capacities of their respective Golgi.
30914498	4	21	theme	cell	1093:1096	arg1	walls					1098:1102	their respective cell walls	1076:1102	their respective cell walls	1076:1102	Further, hemicelluloses accumulated in Golgi at levels that contrasted with those found in their respective cell walls, with xyloglucans enriched in maize Golgi, and xylans enriched in Arabidopsis.
30914498	4	22	theme	maize	1134:1138	arg1	Golgi					1140:1144	maize Golgi	1134:1144	maize Golgi	1134:1144	Further, hemicelluloses accumulated in Golgi at levels that contrasted with those found in their respective cell walls, with xyloglucans enriched in maize Golgi, and xylans enriched in Arabidopsis.
30914498	2	23	theme	noncommelinid	383:395	arg1	monocots					397:404	noncommelinid monocots	383:404	noncommelinid monocots	383:404	As grass species make cell walls distinct from those of dicots and noncommelinid monocots, it has been assumed that the differences in cell-wall composition stem from differences in biosynthetic capacities of their respective Golgi.
30914498	0	24	theme	Cell-Wall	100:108	arg1	Structures					110:119	Distinct Cell-Wall Structures	91:119	Distinct Cell-Wall Structures	91:119	Glycome and Proteome Components of Golgi Membranes Are Common between Two Angiosperms with Distinct Cell-Wall Structures.
30914498	6	25	theme	unknown	1704:1710	arg1	mechanism					1712:1720	an as-yet unknown mechanism	1694:1720	an as-yet unknown mechanism	1694:1720	We propose that the distinctive compositions of grass primary cell walls compared with other angiosperms result from differential gating or metabolism of secreted polysaccharides post-Golgi by an as-yet unknown mechanism, and not necessarily by differential expression of genes encoding specific synthase complexes.
30914498	6	26	theme	grass	1549:1553	arg1	walls					1568:1572	grass primary cell walls	1549:1572	grass primary cell walls	1549:1572	We propose that the distinctive compositions of grass primary cell walls compared with other angiosperms result from differential gating or metabolism of secreted polysaccharides post-Golgi by an as-yet unknown mechanism, and not necessarily by differential expression of genes encoding specific synthase complexes.
30914498	5	27	theme	maize	1213:1217	arg1	membranes					1225:1233	maize Golgi membranes	1213:1233	maize Golgi membranes	1213:1233	Consistent with this finding, maize Golgi membranes isolated by flotation centrifugation and enriched further by free-flow electrophoresis, yielded >200 proteins known to function in the biosynthesis and metabolism of cell-wall polysaccharides common to all angiosperms, and not just those specific to cell-wall type.
30914498	5	28	theme	cell-wall	1401:1409	arg1	polysaccharides					1411:1425	cell-wall polysaccharides	1401:1425	cell-wall polysaccharides common to all angiosperms	1401:1451	Consistent with this finding, maize Golgi membranes isolated by flotation centrifugation and enriched further by free-flow electrophoresis, yielded >200 proteins known to function in the biosynthesis and metabolism of cell-wall polysaccharides common to all angiosperms, and not just those specific to cell-wall type.
30914498	3	29	from	membranes	649:657	arg1	analysis					612:619	carbohydrate linkage analysis	591:619	carbohydrate linkage analysis of polysaccharides in Golgi membranes, enriched by flotation centrifugation from etiolated coleoptiles of maize (Zea mays) and leaves of Arabidopsis (Arabidopsis thaliana),	591:792	However, immunosorbence-based screens and carbohydrate linkage analysis of polysaccharides in Golgi membranes, enriched by flotation centrifugation from etiolated coleoptiles of maize (Zea mays) and leaves of Arabidopsis (Arabidopsis thaliana), showed that arabinogalactan-proteins and arabinans represent substantial portions of the Golgi-resident polysaccharides not typically found in high abundance in cell walls of either species.
30914498	3	29	from	membranes	649:657	arg1	screens					579:585	immunosorbence-based screens	558:585	immunosorbence-based screens	558:585	However, immunosorbence-based screens and carbohydrate linkage analysis of polysaccharides in Golgi membranes, enriched by flotation centrifugation from etiolated coleoptiles of maize (Zea mays) and leaves of Arabidopsis (Arabidopsis thaliana), showed that arabinogalactan-proteins and arabinans represent substantial portions of the Golgi-resident polysaccharides not typically found in high abundance in cell walls of either species.
30914498	1	30	theme	synthesis	185:193	arg1	synthesis					185:193	synthesis	185:193	synthesis	185:193	The plant endoplasmic reticulum-Golgi apparatus is the site of synthesis, assembly, and trafficking of all noncellulosic polysaccharides, proteoglycans, and proteins destined for the cell wall.
30914498	1	30	theme	synthesis	185:193	arg1	apparatus					160:168	The plant endoplasmic reticulum-Golgi apparatus	122:168	The plant endoplasmic reticulum-Golgi apparatus	122:168	The plant endoplasmic reticulum-Golgi apparatus is the site of synthesis, assembly, and trafficking of all noncellulosic polysaccharides, proteoglycans, and proteins destined for the cell wall.
30914498	1	30	theme	synthesis	185:193	arg1	site					177:180	the site	173:180	the site of synthesis, assembly, and trafficking of all noncellulosic polysaccharides, proteoglycans, and proteins destined for the cell wall	173:313	The plant endoplasmic reticulum-Golgi apparatus is the site of synthesis, assembly, and trafficking of all noncellulosic polysaccharides, proteoglycans, and proteins destined for the cell wall.
30914498	1	30	theme	synthesis	185:193	arg1	assembly					196:203	assembly	196:203	assembly	196:203	The plant endoplasmic reticulum-Golgi apparatus is the site of synthesis, assembly, and trafficking of all noncellulosic polysaccharides, proteoglycans, and proteins destined for the cell wall.
30914498	1	30	theme	synthesis	185:193	arg1	trafficking					210:220	trafficking	210:220	trafficking	210:220	The plant endoplasmic reticulum-Golgi apparatus is the site of synthesis, assembly, and trafficking of all noncellulosic polysaccharides, proteoglycans, and proteins destined for the cell wall.
30914498	0	31	theme	Glycome	0:6	arg1	Components					21:30	Glycome and Proteome Components	0:30	Glycome and Proteome Components of Golgi Membranes	0:49	Glycome and Proteome Components of Golgi Membranes Are Common between Two Angiosperms with Distinct Cell-Wall Structures.
30914498	1	32	theme	proteins	279:286	arg1	synthesis					185:193	synthesis	185:193	synthesis	185:193	The plant endoplasmic reticulum-Golgi apparatus is the site of synthesis, assembly, and trafficking of all noncellulosic polysaccharides, proteoglycans, and proteins destined for the cell wall.
30914498	1	32	theme	proteins	279:286	arg1	assembly					196:203	assembly	196:203	assembly	196:203	The plant endoplasmic reticulum-Golgi apparatus is the site of synthesis, assembly, and trafficking of all noncellulosic polysaccharides, proteoglycans, and proteins destined for the cell wall.
30914498	1	32	theme	proteins	279:286	arg1	trafficking					210:220	trafficking	210:220	trafficking	210:220	The plant endoplasmic reticulum-Golgi apparatus is the site of synthesis, assembly, and trafficking of all noncellulosic polysaccharides, proteoglycans, and proteins destined for the cell wall.
30914498	2	33	from	those	363:367	arg1	distinct					349:356	distinct	349:356	distinct	349:356	As grass species make cell walls distinct from those of dicots and noncommelinid monocots, it has been assumed that the differences in cell-wall composition stem from differences in biosynthetic capacities of their respective Golgi.
30914498	6	34	theme	differential	1746:1757	arg1	expression					1759:1768	differential expression	1746:1768	differential expression of genes encoding specific synthase complexes	1746:1814	We propose that the distinctive compositions of grass primary cell walls compared with other angiosperms result from differential gating or metabolism of secreted polysaccharides post-Golgi by an as-yet unknown mechanism, and not necessarily by differential expression of genes encoding specific synthase complexes.
30914498	3	35	theme	species	976:982	arg1	walls					960:964	cell walls	955:964	cell walls of either species	955:982	However, immunosorbence-based screens and carbohydrate linkage analysis of polysaccharides in Golgi membranes, enriched by flotation centrifugation from etiolated coleoptiles of maize (Zea mays) and leaves of Arabidopsis (Arabidopsis thaliana), showed that arabinogalactan-proteins and arabinans represent substantial portions of the Golgi-resident polysaccharides not typically found in high abundance in cell walls of either species.
30914498	4	36	theme	respective	1082:1091	arg1	walls					1098:1102	their respective cell walls	1076:1102	their respective cell walls	1076:1102	Further, hemicelluloses accumulated in Golgi at levels that contrasted with those found in their respective cell walls, with xyloglucans enriched in maize Golgi, and xylans enriched in Arabidopsis.
30914498	3	37	theme	Arabidopsis	771:781	arg1	Arabidopsis					758:768	Arabidopsis	758:768	Arabidopsis	758:768	However, immunosorbence-based screens and carbohydrate linkage analysis of polysaccharides in Golgi membranes, enriched by flotation centrifugation from etiolated coleoptiles of maize (Zea mays) and leaves of Arabidopsis (Arabidopsis thaliana), showed that arabinogalactan-proteins and arabinans represent substantial portions of the Golgi-resident polysaccharides not typically found in high abundance in cell walls of either species.
30914498	3	37	theme	Arabidopsis	771:781	arg1	thaliana					783:790	Arabidopsis thaliana	771:790	Arabidopsis thaliana	771:790	However, immunosorbence-based screens and carbohydrate linkage analysis of polysaccharides in Golgi membranes, enriched by flotation centrifugation from etiolated coleoptiles of maize (Zea mays) and leaves of Arabidopsis (Arabidopsis thaliana), showed that arabinogalactan-proteins and arabinans represent substantial portions of the Golgi-resident polysaccharides not typically found in high abundance in cell walls of either species.
30914498	3	38	theme	substantial	855:865	arg1	portions					867:874	substantial portions	855:874	substantial portions of the Golgi-resident polysaccharides not typically found in high abundance in cell walls of either species	855:982	However, immunosorbence-based screens and carbohydrate linkage analysis of polysaccharides in Golgi membranes, enriched by flotation centrifugation from etiolated coleoptiles of maize (Zea mays) and leaves of Arabidopsis (Arabidopsis thaliana), showed that arabinogalactan-proteins and arabinans represent substantial portions of the Golgi-resident polysaccharides not typically found in high abundance in cell walls of either species.
30914498	4	39	located	found	1067:1071	arg2	those					1061:1065	those	1061:1065	those	1061:1065	Further, hemicelluloses accumulated in Golgi at levels that contrasted with those found in their respective cell walls, with xyloglucans enriched in maize Golgi, and xylans enriched in Arabidopsis.
30914498	4	39	located	found	1067:1071	arg1	walls					1098:1102	their respective cell walls	1076:1102	their respective cell walls	1076:1102	Further, hemicelluloses accumulated in Golgi at levels that contrasted with those found in their respective cell walls, with xyloglucans enriched in maize Golgi, and xylans enriched in Arabidopsis.
30914498	1	40	theme	assembly	196:203	arg1	synthesis					185:193	synthesis	185:193	synthesis	185:193	The plant endoplasmic reticulum-Golgi apparatus is the site of synthesis, assembly, and trafficking of all noncellulosic polysaccharides, proteoglycans, and proteins destined for the cell wall.
30914498	1	40	theme	assembly	196:203	arg1	apparatus					160:168	The plant endoplasmic reticulum-Golgi apparatus	122:168	The plant endoplasmic reticulum-Golgi apparatus	122:168	The plant endoplasmic reticulum-Golgi apparatus is the site of synthesis, assembly, and trafficking of all noncellulosic polysaccharides, proteoglycans, and proteins destined for the cell wall.
30914498	1	40	theme	assembly	196:203	arg1	site					177:180	the site	173:180	the site of synthesis, assembly, and trafficking of all noncellulosic polysaccharides, proteoglycans, and proteins destined for the cell wall	173:313	The plant endoplasmic reticulum-Golgi apparatus is the site of synthesis, assembly, and trafficking of all noncellulosic polysaccharides, proteoglycans, and proteins destined for the cell wall.
30914498	1	40	theme	assembly	196:203	arg1	assembly					196:203	assembly	196:203	assembly	196:203	The plant endoplasmic reticulum-Golgi apparatus is the site of synthesis, assembly, and trafficking of all noncellulosic polysaccharides, proteoglycans, and proteins destined for the cell wall.
30914498	1	40	theme	assembly	196:203	arg1	trafficking					210:220	trafficking	210:220	trafficking	210:220	The plant endoplasmic reticulum-Golgi apparatus is the site of synthesis, assembly, and trafficking of all noncellulosic polysaccharides, proteoglycans, and proteins destined for the cell wall.
30914498	0	41	theme	Proteome	12:19	arg1	Components					21:30	Glycome and Proteome Components	0:30	Glycome and Proteome Components of Golgi Membranes	0:49	Glycome and Proteome Components of Golgi Membranes Are Common between Two Angiosperms with Distinct Cell-Wall Structures.
30914498	5	42	theme	Golgi	1219:1223	arg1	membranes					1225:1233	maize Golgi membranes	1213:1233	maize Golgi membranes	1213:1233	Consistent with this finding, maize Golgi membranes isolated by flotation centrifugation and enriched further by free-flow electrophoresis, yielded >200 proteins known to function in the biosynthesis and metabolism of cell-wall polysaccharides common to all angiosperms, and not just those specific to cell-wall type.
30914498	6	43	theme	specific	1788:1795	arg1	complexes					1806:1814	specific synthase complexes	1788:1814	specific synthase complexes	1788:1814	We propose that the distinctive compositions of grass primary cell walls compared with other angiosperms result from differential gating or metabolism of secreted polysaccharides post-Golgi by an as-yet unknown mechanism, and not necessarily by differential expression of genes encoding specific synthase complexes.
30914498	3	44	theme	immunosorbence-based	558:577	arg1	screens					579:585	immunosorbence-based screens	558:585	immunosorbence-based screens	558:585	However, immunosorbence-based screens and carbohydrate linkage analysis of polysaccharides in Golgi membranes, enriched by flotation centrifugation from etiolated coleoptiles of maize (Zea mays) and leaves of Arabidopsis (Arabidopsis thaliana), showed that arabinogalactan-proteins and arabinans represent substantial portions of the Golgi-resident polysaccharides not typically found in high abundance in cell walls of either species.
30914498	3	45	from	analysis	612:619	arg1	membranes					649:657	Golgi membranes	643:657	Golgi membranes	643:657	However, immunosorbence-based screens and carbohydrate linkage analysis of polysaccharides in Golgi membranes, enriched by flotation centrifugation from etiolated coleoptiles of maize (Zea mays) and leaves of Arabidopsis (Arabidopsis thaliana), showed that arabinogalactan-proteins and arabinans represent substantial portions of the Golgi-resident polysaccharides not typically found in high abundance in cell walls of either species.
30914498	0	46	theme	Golgi	35:39	arg1	Membranes					41:49	Golgi Membranes	35:49	Golgi Membranes	35:49	Glycome and Proteome Components of Golgi Membranes Are Common between Two Angiosperms with Distinct Cell-Wall Structures.
30914498	2	47	theme	Golgi	542:546	arg1	capacities					511:520	biosynthetic capacities	498:520	biosynthetic capacities of their respective Golgi	498:546	As grass species make cell walls distinct from those of dicots and noncommelinid monocots, it has been assumed that the differences in cell-wall composition stem from differences in biosynthetic capacities of their respective Golgi.
30914498	3	48	theme	flotation	672:680	arg1	centrifugation					682:695	flotation centrifugation	672:695	flotation centrifugation	672:695	However, immunosorbence-based screens and carbohydrate linkage analysis of polysaccharides in Golgi membranes, enriched by flotation centrifugation from etiolated coleoptiles of maize (Zea mays) and leaves of Arabidopsis (Arabidopsis thaliana), showed that arabinogalactan-proteins and arabinans represent substantial portions of the Golgi-resident polysaccharides not typically found in high abundance in cell walls of either species.
30914498	5	49	dep	biosynthesis	1370:1381	arg1	the					1366:1368	the	1366:1368	the	1366:1368	Consistent with this finding, maize Golgi membranes isolated by flotation centrifugation and enriched further by free-flow electrophoresis, yielded >200 proteins known to function in the biosynthesis and metabolism of cell-wall polysaccharides common to all angiosperms, and not just those specific to cell-wall type.
30914498	2	50	theme	cell-wall	451:459	arg1	composition					461:471	cell-wall composition	451:471	cell-wall composition	451:471	As grass species make cell walls distinct from those of dicots and noncommelinid monocots, it has been assumed that the differences in cell-wall composition stem from differences in biosynthetic capacities of their respective Golgi.
30914498	1	51	theme	cell	305:308	arg1	wall					310:313	the cell wall	301:313	the cell wall	301:313	The plant endoplasmic reticulum-Golgi apparatus is the site of synthesis, assembly, and trafficking of all noncellulosic polysaccharides, proteoglycans, and proteins destined for the cell wall.
30914498	2	52	theme	respective	531:540	arg1	Golgi					542:546	their respective Golgi	525:546	their respective Golgi	525:546	As grass species make cell walls distinct from those of dicots and noncommelinid monocots, it has been assumed that the differences in cell-wall composition stem from differences in biosynthetic capacities of their respective Golgi.
30914498	6	53	theme	genes	1773:1777	arg1	expression					1759:1768	differential expression	1746:1768	differential expression of genes encoding specific synthase complexes	1746:1814	We propose that the distinctive compositions of grass primary cell walls compared with other angiosperms result from differential gating or metabolism of secreted polysaccharides post-Golgi by an as-yet unknown mechanism, and not necessarily by differential expression of genes encoding specific synthase complexes.
30914498	6	54	dep	necessarily	1731:1741	arg1	not					1727:1729	not	1727:1729	not	1727:1729	We propose that the distinctive compositions of grass primary cell walls compared with other angiosperms result from differential gating or metabolism of secreted polysaccharides post-Golgi by an as-yet unknown mechanism, and not necessarily by differential expression of genes encoding specific synthase complexes.
30914498	1	55	theme	trafficking	210:220	arg1	synthesis					185:193	synthesis	185:193	synthesis	185:193	The plant endoplasmic reticulum-Golgi apparatus is the site of synthesis, assembly, and trafficking of all noncellulosic polysaccharides, proteoglycans, and proteins destined for the cell wall.
30914498	1	55	theme	trafficking	210:220	arg1	apparatus					160:168	The plant endoplasmic reticulum-Golgi apparatus	122:168	The plant endoplasmic reticulum-Golgi apparatus	122:168	The plant endoplasmic reticulum-Golgi apparatus is the site of synthesis, assembly, and trafficking of all noncellulosic polysaccharides, proteoglycans, and proteins destined for the cell wall.
30914498	1	55	theme	trafficking	210:220	arg1	site					177:180	the site	173:180	the site of synthesis, assembly, and trafficking of all noncellulosic polysaccharides, proteoglycans, and proteins destined for the cell wall	173:313	The plant endoplasmic reticulum-Golgi apparatus is the site of synthesis, assembly, and trafficking of all noncellulosic polysaccharides, proteoglycans, and proteins destined for the cell wall.
30914498	1	55	theme	trafficking	210:220	arg1	assembly					196:203	assembly	196:203	assembly	196:203	The plant endoplasmic reticulum-Golgi apparatus is the site of synthesis, assembly, and trafficking of all noncellulosic polysaccharides, proteoglycans, and proteins destined for the cell wall.
30914498	1	55	theme	trafficking	210:220	arg1	trafficking					210:220	trafficking	210:220	trafficking	210:220	The plant endoplasmic reticulum-Golgi apparatus is the site of synthesis, assembly, and trafficking of all noncellulosic polysaccharides, proteoglycans, and proteins destined for the cell wall.
30914498	6	56	theme	primary	1555:1561	arg1	walls					1568:1572	grass primary cell walls	1549:1572	grass primary cell walls	1549:1572	We propose that the distinctive compositions of grass primary cell walls compared with other angiosperms result from differential gating or metabolism of secreted polysaccharides post-Golgi by an as-yet unknown mechanism, and not necessarily by differential expression of genes encoding specific synthase complexes.
30914498	2	57	from	differences	483:493	arg1	capacities					511:520	biosynthetic capacities	498:520	biosynthetic capacities of their respective Golgi	498:546	As grass species make cell walls distinct from those of dicots and noncommelinid monocots, it has been assumed that the differences in cell-wall composition stem from differences in biosynthetic capacities of their respective Golgi.
30914498	3	58	located	found	928:932	arg1	abundance					942:950	high abundance	937:950	high abundance in cell walls of either species	937:982	However, immunosorbence-based screens and carbohydrate linkage analysis of polysaccharides in Golgi membranes, enriched by flotation centrifugation from etiolated coleoptiles of maize (Zea mays) and leaves of Arabidopsis (Arabidopsis thaliana), showed that arabinogalactan-proteins and arabinans represent substantial portions of the Golgi-resident polysaccharides not typically found in high abundance in cell walls of either species.
30914498	3	58	located	found	928:932	arg2	polysaccharides					898:912	the Golgi-resident polysaccharides	879:912	the Golgi-resident polysaccharides not typically found in high abundance in cell walls of either species	879:982	However, immunosorbence-based screens and carbohydrate linkage analysis of polysaccharides in Golgi membranes, enriched by flotation centrifugation from etiolated coleoptiles of maize (Zea mays) and leaves of Arabidopsis (Arabidopsis thaliana), showed that arabinogalactan-proteins and arabinans represent substantial portions of the Golgi-resident polysaccharides not typically found in high abundance in cell walls of either species.
30914498	6	59	theme	cell	1563:1566	arg1	walls					1568:1572	grass primary cell walls	1549:1572	grass primary cell walls	1549:1572	We propose that the distinctive compositions of grass primary cell walls compared with other angiosperms result from differential gating or metabolism of secreted polysaccharides post-Golgi by an as-yet unknown mechanism, and not necessarily by differential expression of genes encoding specific synthase complexes.
30914498	2	60	theme	cell	338:341	arg1	walls					343:347	cell walls	338:347	cell walls distinct from those of dicots and noncommelinid monocots	338:404	As grass species make cell walls distinct from those of dicots and noncommelinid monocots, it has been assumed that the differences in cell-wall composition stem from differences in biosynthetic capacities of their respective Golgi.
30914498	0	61	theme	Membranes	41:49	arg1	Components					21:30	Glycome and Proteome Components	0:30	Glycome and Proteome Components of Golgi Membranes	0:49	Glycome and Proteome Components of Golgi Membranes Are Common between Two Angiosperms with Distinct Cell-Wall Structures.
30914498	3	62	theme	etiolated	702:710	arg1	coleoptiles					712:722	etiolated coleoptiles	702:722	etiolated coleoptiles of maize (Zea mays)	702:742	However, immunosorbence-based screens and carbohydrate linkage analysis of polysaccharides in Golgi membranes, enriched by flotation centrifugation from etiolated coleoptiles of maize (Zea mays) and leaves of Arabidopsis (Arabidopsis thaliana), showed that arabinogalactan-proteins and arabinans represent substantial portions of the Golgi-resident polysaccharides not typically found in high abundance in cell walls of either species.
30914498	3	63	theme	linkage	604:610	arg1	analysis					612:619	carbohydrate linkage analysis	591:619	carbohydrate linkage analysis of polysaccharides in Golgi membranes, enriched by flotation centrifugation from etiolated coleoptiles of maize (Zea mays) and leaves of Arabidopsis (Arabidopsis thaliana),	591:792	However, immunosorbence-based screens and carbohydrate linkage analysis of polysaccharides in Golgi membranes, enriched by flotation centrifugation from etiolated coleoptiles of maize (Zea mays) and leaves of Arabidopsis (Arabidopsis thaliana), showed that arabinogalactan-proteins and arabinans represent substantial portions of the Golgi-resident polysaccharides not typically found in high abundance in cell walls of either species.
30914498	6	64	theme	secreted	1655:1662	arg1	polysaccharides					1664:1678	secreted polysaccharides	1655:1678	secreted polysaccharides post-Golgi by an as-yet unknown mechanism, and not necessarily by differential expression of genes encoding specific synthase complexes	1655:1814	We propose that the distinctive compositions of grass primary cell walls compared with other angiosperms result from differential gating or metabolism of secreted polysaccharides post-Golgi by an as-yet unknown mechanism, and not necessarily by differential expression of genes encoding specific synthase complexes.
30914498	3	65	theme	polysaccharides	898:912	arg1	portions					867:874	substantial portions	855:874	substantial portions of the Golgi-resident polysaccharides not typically found in high abundance in cell walls of either species	855:982	However, immunosorbence-based screens and carbohydrate linkage analysis of polysaccharides in Golgi membranes, enriched by flotation centrifugation from etiolated coleoptiles of maize (Zea mays) and leaves of Arabidopsis (Arabidopsis thaliana), showed that arabinogalactan-proteins and arabinans represent substantial portions of the Golgi-resident polysaccharides not typically found in high abundance in cell walls of either species.
30914498	3	66	from	abundance	942:950	arg1	walls					960:964	cell walls	955:964	cell walls of either species	955:982	However, immunosorbence-based screens and carbohydrate linkage analysis of polysaccharides in Golgi membranes, enriched by flotation centrifugation from etiolated coleoptiles of maize (Zea mays) and leaves of Arabidopsis (Arabidopsis thaliana), showed that arabinogalactan-proteins and arabinans represent substantial portions of the Golgi-resident polysaccharides not typically found in high abundance in cell walls of either species.
30914498	3	67	theme	Golgi	643:647	arg1	membranes					649:657	Golgi membranes	643:657	Golgi membranes	643:657	However, immunosorbence-based screens and carbohydrate linkage analysis of polysaccharides in Golgi membranes, enriched by flotation centrifugation from etiolated coleoptiles of maize (Zea mays) and leaves of Arabidopsis (Arabidopsis thaliana), showed that arabinogalactan-proteins and arabinans represent substantial portions of the Golgi-resident polysaccharides not typically found in high abundance in cell walls of either species.
30914498	1	68	theme	plant	126:130	arg1	synthesis					185:193	synthesis	185:193	synthesis	185:193	The plant endoplasmic reticulum-Golgi apparatus is the site of synthesis, assembly, and trafficking of all noncellulosic polysaccharides, proteoglycans, and proteins destined for the cell wall.
30914498	1	68	theme	plant	126:130	arg1	apparatus					160:168	The plant endoplasmic reticulum-Golgi apparatus	122:168	The plant endoplasmic reticulum-Golgi apparatus	122:168	The plant endoplasmic reticulum-Golgi apparatus is the site of synthesis, assembly, and trafficking of all noncellulosic polysaccharides, proteoglycans, and proteins destined for the cell wall.
30914498	1	68	theme	plant	126:130	arg1	site					177:180	the site	173:180	the site of synthesis, assembly, and trafficking of all noncellulosic polysaccharides, proteoglycans, and proteins destined for the cell wall	173:313	The plant endoplasmic reticulum-Golgi apparatus is the site of synthesis, assembly, and trafficking of all noncellulosic polysaccharides, proteoglycans, and proteins destined for the cell wall.
30914498	1	68	theme	plant	126:130	arg1	assembly					196:203	assembly	196:203	assembly	196:203	The plant endoplasmic reticulum-Golgi apparatus is the site of synthesis, assembly, and trafficking of all noncellulosic polysaccharides, proteoglycans, and proteins destined for the cell wall.
30914498	1	68	theme	plant	126:130	arg1	trafficking					210:220	trafficking	210:220	trafficking	210:220	The plant endoplasmic reticulum-Golgi apparatus is the site of synthesis, assembly, and trafficking of all noncellulosic polysaccharides, proteoglycans, and proteins destined for the cell wall.
30914498	2	69	theme	biosynthetic	498:509	arg1	capacities					511:520	biosynthetic capacities	498:520	biosynthetic capacities of their respective Golgi	498:546	As grass species make cell walls distinct from those of dicots and noncommelinid monocots, it has been assumed that the differences in cell-wall composition stem from differences in biosynthetic capacities of their respective Golgi.
30914498	6	70	theme	differential	1618:1629	arg1	gating					1631:1636	differential gating	1618:1636	differential gating	1618:1636	We propose that the distinctive compositions of grass primary cell walls compared with other angiosperms result from differential gating or metabolism of secreted polysaccharides post-Golgi by an as-yet unknown mechanism, and not necessarily by differential expression of genes encoding specific synthase complexes.
30914498	3	71	from	polysaccharides	624:638	arg1	membranes					649:657	Golgi membranes	643:657	Golgi membranes	643:657	However, immunosorbence-based screens and carbohydrate linkage analysis of polysaccharides in Golgi membranes, enriched by flotation centrifugation from etiolated coleoptiles of maize (Zea mays) and leaves of Arabidopsis (Arabidopsis thaliana), showed that arabinogalactan-proteins and arabinans represent substantial portions of the Golgi-resident polysaccharides not typically found in high abundance in cell walls of either species.
30914498	2	72	theme	grass	319:323	arg1	species					325:331	grass species	319:331	grass species	319:331	As grass species make cell walls distinct from those of dicots and noncommelinid monocots, it has been assumed that the differences in cell-wall composition stem from differences in biosynthetic capacities of their respective Golgi.
30914498	3	73	from	screens	579:585	arg1	membranes					649:657	Golgi membranes	643:657	Golgi membranes	643:657	However, immunosorbence-based screens and carbohydrate linkage analysis of polysaccharides in Golgi membranes, enriched by flotation centrifugation from etiolated coleoptiles of maize (Zea mays) and leaves of Arabidopsis (Arabidopsis thaliana), showed that arabinogalactan-proteins and arabinans represent substantial portions of the Golgi-resident polysaccharides not typically found in high abundance in cell walls of either species.
30914498	3	74	dep	membranes	649:657	arg1	enriched					660:667	enriched	660:667	enriched by flotation centrifugation from etiolated coleoptiles of maize (Zea mays)	660:742	However, immunosorbence-based screens and carbohydrate linkage analysis of polysaccharides in Golgi membranes, enriched by flotation centrifugation from etiolated coleoptiles of maize (Zea mays) and leaves of Arabidopsis (Arabidopsis thaliana), showed that arabinogalactan-proteins and arabinans represent substantial portions of the Golgi-resident polysaccharides not typically found in high abundance in cell walls of either species.
30914498	3	74	dep	membranes	649:657	arg1	leaves					748:753	leaves	748:753	leaves of Arabidopsis (Arabidopsis thaliana)	748:791	However, immunosorbence-based screens and carbohydrate linkage analysis of polysaccharides in Golgi membranes, enriched by flotation centrifugation from etiolated coleoptiles of maize (Zea mays) and leaves of Arabidopsis (Arabidopsis thaliana), showed that arabinogalactan-proteins and arabinans represent substantial portions of the Golgi-resident polysaccharides not typically found in high abundance in cell walls of either species.
30914498	3	75	theme	polysaccharides	624:638	arg1	analysis					612:619	carbohydrate linkage analysis	591:619	carbohydrate linkage analysis of polysaccharides in Golgi membranes, enriched by flotation centrifugation from etiolated coleoptiles of maize (Zea mays) and leaves of Arabidopsis (Arabidopsis thaliana),	591:792	However, immunosorbence-based screens and carbohydrate linkage analysis of polysaccharides in Golgi membranes, enriched by flotation centrifugation from etiolated coleoptiles of maize (Zea mays) and leaves of Arabidopsis (Arabidopsis thaliana), showed that arabinogalactan-proteins and arabinans represent substantial portions of the Golgi-resident polysaccharides not typically found in high abundance in cell walls of either species.
30914498	3	75	theme	polysaccharides	624:638	arg1	screens					579:585	immunosorbence-based screens	558:585	immunosorbence-based screens	558:585	However, immunosorbence-based screens and carbohydrate linkage analysis of polysaccharides in Golgi membranes, enriched by flotation centrifugation from etiolated coleoptiles of maize (Zea mays) and leaves of Arabidopsis (Arabidopsis thaliana), showed that arabinogalactan-proteins and arabinans represent substantial portions of the Golgi-resident polysaccharides not typically found in high abundance in cell walls of either species.
30914498	5	76	theme	common	1427:1432	arg1	polysaccharides					1411:1425	cell-wall polysaccharides	1401:1425	cell-wall polysaccharides common to all angiosperms	1401:1451	Consistent with this finding, maize Golgi membranes isolated by flotation centrifugation and enriched further by free-flow electrophoresis, yielded >200 proteins known to function in the biosynthesis and metabolism of cell-wall polysaccharides common to all angiosperms, and not just those specific to cell-wall type.
30914498	1	77	theme	reticulum-Golgi	144:158	arg1	synthesis					185:193	synthesis	185:193	synthesis	185:193	The plant endoplasmic reticulum-Golgi apparatus is the site of synthesis, assembly, and trafficking of all noncellulosic polysaccharides, proteoglycans, and proteins destined for the cell wall.
30914498	1	77	theme	reticulum-Golgi	144:158	arg1	apparatus					160:168	The plant endoplasmic reticulum-Golgi apparatus	122:168	The plant endoplasmic reticulum-Golgi apparatus	122:168	The plant endoplasmic reticulum-Golgi apparatus is the site of synthesis, assembly, and trafficking of all noncellulosic polysaccharides, proteoglycans, and proteins destined for the cell wall.
30914498	1	77	theme	reticulum-Golgi	144:158	arg1	site					177:180	the site	173:180	the site of synthesis, assembly, and trafficking of all noncellulosic polysaccharides, proteoglycans, and proteins destined for the cell wall	173:313	The plant endoplasmic reticulum-Golgi apparatus is the site of synthesis, assembly, and trafficking of all noncellulosic polysaccharides, proteoglycans, and proteins destined for the cell wall.
30914498	1	77	theme	reticulum-Golgi	144:158	arg1	assembly					196:203	assembly	196:203	assembly	196:203	The plant endoplasmic reticulum-Golgi apparatus is the site of synthesis, assembly, and trafficking of all noncellulosic polysaccharides, proteoglycans, and proteins destined for the cell wall.
30914498	1	77	theme	reticulum-Golgi	144:158	arg1	trafficking					210:220	trafficking	210:220	trafficking	210:220	The plant endoplasmic reticulum-Golgi apparatus is the site of synthesis, assembly, and trafficking of all noncellulosic polysaccharides, proteoglycans, and proteins destined for the cell wall.
30914498	3	78	theme	Golgi-resident	883:896	arg1	polysaccharides					898:912	the Golgi-resident polysaccharides	879:912	the Golgi-resident polysaccharides not typically found in high abundance in cell walls of either species	879:982	However, immunosorbence-based screens and carbohydrate linkage analysis of polysaccharides in Golgi membranes, enriched by flotation centrifugation from etiolated coleoptiles of maize (Zea mays) and leaves of Arabidopsis (Arabidopsis thaliana), showed that arabinogalactan-proteins and arabinans represent substantial portions of the Golgi-resident polysaccharides not typically found in high abundance in cell walls of either species.
30914498	1	79	theme	noncellulosic	229:241	arg1	polysaccharides					243:257	all noncellulosic polysaccharides	225:257	all noncellulosic polysaccharides	225:257	The plant endoplasmic reticulum-Golgi apparatus is the site of synthesis, assembly, and trafficking of all noncellulosic polysaccharides, proteoglycans, and proteins destined for the cell wall.
30914498	6	80	theme	distinctive	1521:1531	arg1	compositions					1533:1544	the distinctive compositions	1517:1544	the distinctive compositions of grass primary cell walls compared with other angiosperms	1517:1604	We propose that the distinctive compositions of grass primary cell walls compared with other angiosperms result from differential gating or metabolism of secreted polysaccharides post-Golgi by an as-yet unknown mechanism, and not necessarily by differential expression of genes encoding specific synthase complexes.
30914498	6	81	dep	polysaccharides	1664:1678	arg1	post-Golgi					1680:1689	post-Golgi	1680:1689	post-Golgi	1680:1689	We propose that the distinctive compositions of grass primary cell walls compared with other angiosperms result from differential gating or metabolism of secreted polysaccharides post-Golgi by an as-yet unknown mechanism, and not necessarily by differential expression of genes encoding specific synthase complexes.
30914498	5	82	theme	free-flow	1296:1304	arg1	electrophoresis					1306:1320	free-flow electrophoresis	1296:1320	free-flow electrophoresis	1296:1320	Consistent with this finding, maize Golgi membranes isolated by flotation centrifugation and enriched further by free-flow electrophoresis, yielded >200 proteins known to function in the biosynthesis and metabolism of cell-wall polysaccharides common to all angiosperms, and not just those specific to cell-wall type.
30914498	1	83	theme	polysaccharides	243:257	arg1	synthesis					185:193	synthesis	185:193	synthesis	185:193	The plant endoplasmic reticulum-Golgi apparatus is the site of synthesis, assembly, and trafficking of all noncellulosic polysaccharides, proteoglycans, and proteins destined for the cell wall.
30914498	1	83	theme	polysaccharides	243:257	arg1	assembly					196:203	assembly	196:203	assembly	196:203	The plant endoplasmic reticulum-Golgi apparatus is the site of synthesis, assembly, and trafficking of all noncellulosic polysaccharides, proteoglycans, and proteins destined for the cell wall.
30914498	1	83	theme	polysaccharides	243:257	arg1	trafficking					210:220	trafficking	210:220	trafficking	210:220	The plant endoplasmic reticulum-Golgi apparatus is the site of synthesis, assembly, and trafficking of all noncellulosic polysaccharides, proteoglycans, and proteins destined for the cell wall.
31472351	11	0	theme	Vitamin	1456:1462	arg1	synthesis					1467:1475	Vitamin B2 synthesis	1456:1475	Vitamin B2 synthesis	1456:1475	The up-regulation of carotenoid and Vitamin B2 synthesis at 10 °C might also be involved in the resistance to oxidative stress.
31472351	3	1	theme	seasonal	254:261	arg1	changes					263:269	seasonal changes	254:269	seasonal changes in ambient temperature, especially rapid temperature drops	254:328	However, seasonal changes in ambient temperature, especially rapid temperature drops exert an adverse effect on in situ bioremediation.
31472351	11	2	theme	B2	1464:1465	arg1	synthesis					1467:1475	Vitamin B2 synthesis	1456:1475	Vitamin B2 synthesis	1456:1475	The up-regulation of carotenoid and Vitamin B2 synthesis at 10 °C might also be involved in the resistance to oxidative stress.
31472351	4	3	theme	cell	411:414	arg1	morphology					416:425	cell morphology	411:425	cell morphology	411:425	In this paper, we studied the cell morphology and fatty acid composition of an aniline-degrading strain Rhodococcus sp.
31472351	9	4	theme	fatty	1243:1247	arg1	acids					1249:1253	fatty acids	1243:1253	fatty acids	1243:1253	The inhibition of peptidoglycan synthase expression and up-regulation of Filamentous Temperature Sensitive as well as unsaturated fatty acid synthesis genes at suboptimal temperatures might be closely related to corresponding changes in cell morphology and fatty acids composition.
31472351	7	5	theme	Transcriptomic	730:743	arg1	technologies					745:756	Transcriptomic technologies	730:756	Transcriptomic technologies	730:756	Transcriptomic technologies were then utilized to gain detailed insights into the adaptive mechanisms of CNS16 subjected to suboptimal temperatures.
31472351	9	6	theme	up-regulation	1042:1054	arg1	related					1187:1193	related	1187:1193	related	1187:1193	The inhibition of peptidoglycan synthase expression and up-regulation of Filamentous Temperature Sensitive as well as unsaturated fatty acid synthesis genes at suboptimal temperatures might be closely related to corresponding changes in cell morphology and fatty acids composition.
31472351	9	6	theme	up-regulation	1042:1054	arg1	inhibition					990:999	The inhibition	986:999	The inhibition of peptidoglycan synthase expression and up-regulation of Filamentous Temperature Sensitive as well as unsaturated fatty acid synthesis genes at suboptimal temperatures	986:1168	The inhibition of peptidoglycan synthase expression and up-regulation of Filamentous Temperature Sensitive as well as unsaturated fatty acid synthesis genes at suboptimal temperatures might be closely related to corresponding changes in cell morphology and fatty acids composition.
31472351	2	7	theme	environmental	163:175	arg1	stressors					177:185	environmental stressors	163:185	environmental stressors	163:185	Rhodococcus exhibits strong adaptability to environmental stressors and plays a crucial role in environmental bioremediation.
31472351	14	8	from	temperatures	1895:1906	arg1	CNS16					1882:1886	CNS16	1882:1886	CNS16 at low temperatures.	1882:1907	CNS16 at low temperatures.
31472351	7	9	theme	suboptimal	854:863	arg1	temperatures					865:876	suboptimal temperatures	854:876	suboptimal temperatures	854:876	Transcriptomic technologies were then utilized to gain detailed insights into the adaptive mechanisms of CNS16 subjected to suboptimal temperatures.
31472351	3	10	theme	temperature	312:322	arg1	drops					324:328	especially rapid temperature drops	295:328	especially rapid temperature drops	295:328	However, seasonal changes in ambient temperature, especially rapid temperature drops exert an adverse effect on in situ bioremediation.
31472351	3	10	theme	temperature	312:322	arg1	temperature					282:292	ambient temperature	274:292	ambient temperature	274:292	However, seasonal changes in ambient temperature, especially rapid temperature drops exert an adverse effect on in situ bioremediation.
31472351	1	11	dep	CNS16	41:45	arg1	gradients					75:83	different temperature gradients	53:83	different temperature gradients: Physiological and transcriptome	53:116	CNS16 under different temperature gradients: Physiological and transcriptome.
31472351	9	12	theme	peptidoglycan	1004:1016	arg1	expression					1027:1036	peptidoglycan synthase expression	1004:1036	peptidoglycan synthase expression	1004:1036	The inhibition of peptidoglycan synthase expression and up-regulation of Filamentous Temperature Sensitive as well as unsaturated fatty acid synthesis genes at suboptimal temperatures might be closely related to corresponding changes in cell morphology and fatty acids composition.
31472351	11	13	from	10 °C	1480:1484	arg1	up-regulation					1424:1436	The up-regulation	1420:1436	The up-regulation of carotenoid and Vitamin B2 synthesis at 10 °C	1420:1484	The up-regulation of carotenoid and Vitamin B2 synthesis at 10 °C might also be involved in the resistance to oxidative stress.
31472351	4	14	theme	aniline-degrading	460:476	arg1	sp					497:498	an aniline-degrading strain Rhodococcus sp	457:498	an aniline-degrading strain Rhodococcus sp	457:498	In this paper, we studied the cell morphology and fatty acid composition of an aniline-degrading strain Rhodococcus sp.
31472351	9	15	theme	expression	1027:1036	arg1	related					1187:1193	related	1187:1193	related	1187:1193	The inhibition of peptidoglycan synthase expression and up-regulation of Filamentous Temperature Sensitive as well as unsaturated fatty acid synthesis genes at suboptimal temperatures might be closely related to corresponding changes in cell morphology and fatty acids composition.
31472351	9	15	theme	expression	1027:1036	arg1	inhibition					990:999	The inhibition	986:999	The inhibition of peptidoglycan synthase expression and up-regulation of Filamentous Temperature Sensitive as well as unsaturated fatty acid synthesis genes at suboptimal temperatures	986:1168	The inhibition of peptidoglycan synthase expression and up-regulation of Filamentous Temperature Sensitive as well as unsaturated fatty acid synthesis genes at suboptimal temperatures might be closely related to corresponding changes in cell morphology and fatty acids composition.
31472351	3	16	theme	adverse	339:345	arg1	effect					347:352	an adverse effect	336:352	an adverse effect	336:352	However, seasonal changes in ambient temperature, especially rapid temperature drops exert an adverse effect on in situ bioremediation.
31472351	4	17	theme	Rhodococcus	485:495	arg1	sp					497:498	an aniline-degrading strain Rhodococcus sp	457:498	an aniline-degrading strain Rhodococcus sp	457:498	In this paper, we studied the cell morphology and fatty acid composition of an aniline-degrading strain Rhodococcus sp.
31472351	6	18	theme	cell	579:582	arg1	morphology					584:593	cell morphology	579:593	cell morphology of CNS16	579:602	At suboptimal temperatures, cell morphology of CNS16 changed from short rod-shaped to long rod or irregular shaped, and the proportion of unsaturated fatty acids was upregulated.
31472351	12	19	theme	peptidoglycan	1659:1671	arg1	synthesis					1673:1681	peptidoglycan synthesis	1659:1681	peptidoglycan synthesis	1659:1681	Amino acid metabolism, coenzyme and vitamin metabolism, ABC transport, and energy metabolism are essential for peptidoglycan synthesis and regulation of cellular metabolism; therefore, synergistically resisting environmental stress.
31472351	4	20	theme	acid	437:440	arg1	composition					442:452	fatty acid composition	431:452	fatty acid composition	431:452	In this paper, we studied the cell morphology and fatty acid composition of an aniline-degrading strain Rhodococcus sp.
31472351	12	21	theme	metabolism	1710:1719	arg1	regulation					1687:1696	regulation	1687:1696	regulation of cellular metabolism	1687:1719	Amino acid metabolism, coenzyme and vitamin metabolism, ABC transport, and energy metabolism are essential for peptidoglycan synthesis and regulation of cellular metabolism; therefore, synergistically resisting environmental stress.
31472351	12	21	theme	metabolism	1710:1719	arg1	synthesis					1673:1681	peptidoglycan synthesis	1659:1681	peptidoglycan synthesis	1659:1681	Amino acid metabolism, coenzyme and vitamin metabolism, ABC transport, and energy metabolism are essential for peptidoglycan synthesis and regulation of cellular metabolism; therefore, synergistically resisting environmental stress.
31472351	9	22	dep	morphology	1228:1237	arg1	composition					1255:1265	composition	1255:1265	composition	1255:1265	The inhibition of peptidoglycan synthase expression and up-regulation of Filamentous Temperature Sensitive as well as unsaturated fatty acid synthesis genes at suboptimal temperatures might be closely related to corresponding changes in cell morphology and fatty acids composition.
31472351	9	23	theme	Sensitive	1083:1091	arg1	acid					1122:1125	Filamentous Temperature Sensitive as well as unsaturated fatty acid	1059:1125	Filamentous Temperature Sensitive as well as unsaturated fatty acid synthesis	1059:1135	The inhibition of peptidoglycan synthase expression and up-regulation of Filamentous Temperature Sensitive as well as unsaturated fatty acid synthesis genes at suboptimal temperatures might be closely related to corresponding changes in cell morphology and fatty acids composition.
31472351	5	24	theme	10 °C	544:548	arg1	temperatures					510:521	temperatures	510:521	temperatures of 30 °C, 20 °C, and 10 °C	510:548	CNS16 at temperatures of 30 °C, 20 °C, and 10 °C.
31472351	5	25	dep	CNS16	501:505	arg1	temperatures					510:521	temperatures	510:521	temperatures of 30 °C, 20 °C, and 10 °C	510:548	CNS16 at temperatures of 30 °C, 20 °C, and 10 °C.
31472351	10	26	theme	dismutase	1320:1328	arg1	activity					1330:1337	catalase and superoxide dismutase activity	1296:1337	catalase and superoxide dismutase activity	1296:1337	Strain CNS16 showed loss of catalase and superoxide dismutase activity, and utilized thioredoxin-dependent thiol peroxidase to resist oxidative stress.
31472351	9	27	theme	unsaturated	1104:1114	arg1	acid					1122:1125	Filamentous Temperature Sensitive as well as unsaturated fatty acid	1059:1125	Filamentous Temperature Sensitive as well as unsaturated fatty acid synthesis	1059:1135	The inhibition of peptidoglycan synthase expression and up-regulation of Filamentous Temperature Sensitive as well as unsaturated fatty acid synthesis genes at suboptimal temperatures might be closely related to corresponding changes in cell morphology and fatty acids composition.
31472351	1	28	theme	temperature	63:73	arg1	gradients					75:83	different temperature gradients	53:83	different temperature gradients: Physiological and transcriptome	53:116	CNS16 under different temperature gradients: Physiological and transcriptome.
31472351	9	29	theme	acid	1122:1125	arg1	synthesis					1127:1135	Filamentous Temperature Sensitive as well as unsaturated fatty acid synthesis	1059:1135	Filamentous Temperature Sensitive as well as unsaturated fatty acid synthesis	1059:1135	The inhibition of peptidoglycan synthase expression and up-regulation of Filamentous Temperature Sensitive as well as unsaturated fatty acid synthesis genes at suboptimal temperatures might be closely related to corresponding changes in cell morphology and fatty acids composition.
31472351	6	30	theme	fatty	701:705	arg1	acids					707:711	unsaturated fatty acids	689:711	unsaturated fatty acids	689:711	At suboptimal temperatures, cell morphology of CNS16 changed from short rod-shaped to long rod or irregular shaped, and the proportion of unsaturated fatty acids was upregulated.
31472351	0	31	theme	Adaptation	0:9	arg1	mechanisms					11:20	Adaptation mechanisms	0:20	Adaptation mechanisms of Rhodococcus sp	0:38	Adaptation mechanisms of Rhodococcus sp.
31472351	9	32	dep	expression	1027:1036	arg1	genes					1137:1141	genes	1137:1141	genes	1137:1141	The inhibition of peptidoglycan synthase expression and up-regulation of Filamentous Temperature Sensitive as well as unsaturated fatty acid synthesis genes at suboptimal temperatures might be closely related to corresponding changes in cell morphology and fatty acids composition.
31472351	13	33	theme	aniline	1843:1849	arg1	degradation					1851:1861	aniline degradation	1843:1861	aniline degradation	1843:1861	This study provides a mechanistic basis for the regulation of aniline degradation in Rhodococcus sp.
31472351	8	34	theme	responses	922:930	arg1	number					907:912	the number	903:912	the number of gene responses	903:930	The results showed that the number of gene responses was significantly higher at 10 °C than that at 20 °C.
31472351	8	34	theme	responses	922:930	arg1	higher					950:955	higher	950:955	higher	950:955	The results showed that the number of gene responses was significantly higher at 10 °C than that at 20 °C.
31472351	9	35	theme	suboptimal	1146:1155	arg1	temperatures					1157:1168	suboptimal temperatures	1146:1168	suboptimal temperatures	1146:1168	The inhibition of peptidoglycan synthase expression and up-regulation of Filamentous Temperature Sensitive as well as unsaturated fatty acid synthesis genes at suboptimal temperatures might be closely related to corresponding changes in cell morphology and fatty acids composition.
31472351	7	36	theme	adaptive	812:819	arg1	mechanisms					821:830	the adaptive mechanisms	808:830	the adaptive mechanisms of CNS16 subjected to suboptimal temperatures	808:876	Transcriptomic technologies were then utilized to gain detailed insights into the adaptive mechanisms of CNS16 subjected to suboptimal temperatures.
31472351	11	37	theme	oxidative	1530:1538	arg1	stress					1540:1545	oxidative stress	1530:1545	oxidative stress	1530:1545	The up-regulation of carotenoid and Vitamin B2 synthesis at 10 °C might also be involved in the resistance to oxidative stress.
31472351	2	38	theme	crucial	199:205	arg1	role					207:210	a crucial role	197:210	a crucial role	197:210	Rhodococcus exhibits strong adaptability to environmental stressors and plays a crucial role in environmental bioremediation.
31472351	9	39	from	temperatures	1157:1168	arg1	related					1187:1193	related	1187:1193	related	1187:1193	The inhibition of peptidoglycan synthase expression and up-regulation of Filamentous Temperature Sensitive as well as unsaturated fatty acid synthesis genes at suboptimal temperatures might be closely related to corresponding changes in cell morphology and fatty acids composition.
31472351	9	39	from	temperatures	1157:1168	arg1	inhibition					990:999	The inhibition	986:999	The inhibition of peptidoglycan synthase expression and up-regulation of Filamentous Temperature Sensitive as well as unsaturated fatty acid synthesis genes at suboptimal temperatures	986:1168	The inhibition of peptidoglycan synthase expression and up-regulation of Filamentous Temperature Sensitive as well as unsaturated fatty acid synthesis genes at suboptimal temperatures might be closely related to corresponding changes in cell morphology and fatty acids composition.
31472351	10	40	theme	thiol	1375:1379	arg1	peroxidase					1381:1390	thioredoxin-dependent thiol peroxidase	1353:1390	thioredoxin-dependent thiol peroxidase	1353:1390	Strain CNS16 showed loss of catalase and superoxide dismutase activity, and utilized thioredoxin-dependent thiol peroxidase to resist oxidative stress.
31472351	0	41	theme	sp	37:38	arg1	mechanisms					11:20	Adaptation mechanisms	0:20	Adaptation mechanisms of Rhodococcus sp	0:38	Adaptation mechanisms of Rhodococcus sp.
31472351	14	42	theme	low	1891:1893	arg1	temperatures					1895:1906	low temperatures	1891:1906	low temperatures	1891:1906	CNS16 at low temperatures.
31472351	11	43	theme	carotenoid	1441:1450	arg1	up-regulation					1424:1436	The up-regulation	1420:1436	The up-regulation of carotenoid and Vitamin B2 synthesis at 10 °C	1420:1484	The up-regulation of carotenoid and Vitamin B2 synthesis at 10 °C might also be involved in the resistance to oxidative stress.
31472351	7	44	theme	detailed	785:792	arg1	insights					794:801	detailed insights	785:801	detailed insights into the adaptive mechanisms of CNS16 subjected to suboptimal temperatures	785:876	Transcriptomic technologies were then utilized to gain detailed insights into the adaptive mechanisms of CNS16 subjected to suboptimal temperatures.
31472351	12	45	theme	acid	1554:1557	arg1	metabolism					1559:1568	Amino acid metabolism	1548:1568	Amino acid metabolism	1548:1568	Amino acid metabolism, coenzyme and vitamin metabolism, ABC transport, and energy metabolism are essential for peptidoglycan synthesis and regulation of cellular metabolism; therefore, synergistically resisting environmental stress.
31472351	10	46	theme	oxidative	1402:1410	arg1	stress					1412:1417	oxidative stress	1402:1417	oxidative stress	1402:1417	Strain CNS16 showed loss of catalase and superoxide dismutase activity, and utilized thioredoxin-dependent thiol peroxidase to resist oxidative stress.
31472351	11	47	theme	synthesis	1467:1475	arg1	up-regulation					1424:1436	The up-regulation	1420:1436	The up-regulation of carotenoid and Vitamin B2 synthesis at 10 °C	1420:1484	The up-regulation of carotenoid and Vitamin B2 synthesis at 10 °C might also be involved in the resistance to oxidative stress.
31472351	6	48	theme	suboptimal	554:563	arg1	temperatures					565:576	suboptimal temperatures	554:576	suboptimal temperatures	554:576	At suboptimal temperatures, cell morphology of CNS16 changed from short rod-shaped to long rod or irregular shaped, and the proportion of unsaturated fatty acids was upregulated.
31472351	2	49	theme	strong	140:145	arg1	adaptability					147:158	strong adaptability	140:158	strong adaptability	140:158	Rhodococcus exhibits strong adaptability to environmental stressors and plays a crucial role in environmental bioremediation.
31472351	9	50	theme	cell	1223:1226	arg1	morphology					1228:1237	cell morphology	1223:1237	cell morphology	1223:1237	The inhibition of peptidoglycan synthase expression and up-regulation of Filamentous Temperature Sensitive as well as unsaturated fatty acid synthesis genes at suboptimal temperatures might be closely related to corresponding changes in cell morphology and fatty acids composition.
31472351	4	51	theme	fatty	431:435	arg1	composition					442:452	fatty acid composition	431:452	fatty acid composition	431:452	In this paper, we studied the cell morphology and fatty acid composition of an aniline-degrading strain Rhodococcus sp.
31472351	5	52	theme	30 °C	526:530	arg1	temperatures					510:521	temperatures	510:521	temperatures of 30 °C, 20 °C, and 10 °C	510:548	CNS16 at temperatures of 30 °C, 20 °C, and 10 °C.
31472351	13	53	theme	degradation	1851:1861	arg1	regulation					1829:1838	the regulation	1825:1838	the regulation of aniline degradation in Rhodococcus sp	1825:1879	This study provides a mechanistic basis for the regulation of aniline degradation in Rhodococcus sp.
31472351	5	54	theme	20 °C	533:537	arg1	temperatures					510:521	temperatures	510:521	temperatures of 30 °C, 20 °C, and 10 °C	510:548	CNS16 at temperatures of 30 °C, 20 °C, and 10 °C.
31472351	3	55	theme	ambient	274:280	arg1	drops					324:328	especially rapid temperature drops	295:328	especially rapid temperature drops	295:328	However, seasonal changes in ambient temperature, especially rapid temperature drops exert an adverse effect on in situ bioremediation.
31472351	3	55	theme	ambient	274:280	arg1	temperature					282:292	ambient temperature	274:292	ambient temperature	274:292	However, seasonal changes in ambient temperature, especially rapid temperature drops exert an adverse effect on in situ bioremediation.
31472351	9	56	from	changes	1212:1218	arg1	morphology					1228:1237	cell morphology	1223:1237	cell morphology	1223:1237	The inhibition of peptidoglycan synthase expression and up-regulation of Filamentous Temperature Sensitive as well as unsaturated fatty acid synthesis genes at suboptimal temperatures might be closely related to corresponding changes in cell morphology and fatty acids composition.
31472351	9	56	from	changes	1212:1218	arg1	acids					1249:1253	fatty acids	1243:1253	fatty acids	1243:1253	The inhibition of peptidoglycan synthase expression and up-regulation of Filamentous Temperature Sensitive as well as unsaturated fatty acid synthesis genes at suboptimal temperatures might be closely related to corresponding changes in cell morphology and fatty acids composition.
31472351	13	57	theme	Rhodococcus	1866:1876	arg1	sp					1878:1879	Rhodococcus sp	1866:1879	Rhodococcus sp	1866:1879	This study provides a mechanistic basis for the regulation of aniline degradation in Rhodococcus sp.
31472351	12	58	theme	cellular	1701:1708	arg1	metabolism					1710:1719	cellular metabolism	1701:1719	cellular metabolism	1701:1719	Amino acid metabolism, coenzyme and vitamin metabolism, ABC transport, and energy metabolism are essential for peptidoglycan synthesis and regulation of cellular metabolism; therefore, synergistically resisting environmental stress.
31472351	4	59	theme	strain	478:483	arg1	sp					497:498	an aniline-degrading strain Rhodococcus sp	457:498	an aniline-degrading strain Rhodococcus sp	457:498	In this paper, we studied the cell morphology and fatty acid composition of an aniline-degrading strain Rhodococcus sp.
31472351	9	60	theme	synthase	1018:1025	arg1	expression					1027:1036	peptidoglycan synthase expression	1004:1036	peptidoglycan synthase expression	1004:1036	The inhibition of peptidoglycan synthase expression and up-regulation of Filamentous Temperature Sensitive as well as unsaturated fatty acid synthesis genes at suboptimal temperatures might be closely related to corresponding changes in cell morphology and fatty acids composition.
31472351	6	61	theme	long	637:640	arg1	rod					642:644	long rod	637:644	long rod	637:644	At suboptimal temperatures, cell morphology of CNS16 changed from short rod-shaped to long rod or irregular shaped, and the proportion of unsaturated fatty acids was upregulated.
31472351	3	62	theme	rapid	306:310	arg1	drops					324:328	especially rapid temperature drops	295:328	especially rapid temperature drops	295:328	However, seasonal changes in ambient temperature, especially rapid temperature drops exert an adverse effect on in situ bioremediation.
31472351	3	62	theme	rapid	306:310	arg1	temperature					282:292	ambient temperature	274:292	ambient temperature	274:292	However, seasonal changes in ambient temperature, especially rapid temperature drops exert an adverse effect on in situ bioremediation.
31472351	4	63	theme	sp	497:498	arg1	morphology					416:425	cell morphology	411:425	cell morphology	411:425	In this paper, we studied the cell morphology and fatty acid composition of an aniline-degrading strain Rhodococcus sp.
31472351	4	63	theme	sp	497:498	arg1	composition					442:452	fatty acid composition	431:452	fatty acid composition	431:452	In this paper, we studied the cell morphology and fatty acid composition of an aniline-degrading strain Rhodococcus sp.
31472351	10	64	theme	catalase	1296:1303	arg1	activity					1330:1337	catalase and superoxide dismutase activity	1296:1337	catalase and superoxide dismutase activity	1296:1337	Strain CNS16 showed loss of catalase and superoxide dismutase activity, and utilized thioredoxin-dependent thiol peroxidase to resist oxidative stress.
31472351	12	65	theme	energy	1623:1628	arg1	metabolism					1630:1639	energy metabolism	1623:1639	energy metabolism	1623:1639	Amino acid metabolism, coenzyme and vitamin metabolism, ABC transport, and energy metabolism are essential for peptidoglycan synthesis and regulation of cellular metabolism; therefore, synergistically resisting environmental stress.
31472351	10	66	theme	activity	1330:1337	arg1	loss					1288:1291	loss	1288:1291	loss of catalase and superoxide dismutase activity	1288:1337	Strain CNS16 showed loss of catalase and superoxide dismutase activity, and utilized thioredoxin-dependent thiol peroxidase to resist oxidative stress.
31472351	12	67	theme	ABC	1604:1606	arg1	transport					1608:1616	ABC transport	1604:1616	ABC transport	1604:1616	Amino acid metabolism, coenzyme and vitamin metabolism, ABC transport, and energy metabolism are essential for peptidoglycan synthesis and regulation of cellular metabolism; therefore, synergistically resisting environmental stress.
31472351	3	68	theme	in	357:358	arg1	bioremediation					365:378	in situ bioremediation	357:378	in situ bioremediation	357:378	However, seasonal changes in ambient temperature, especially rapid temperature drops exert an adverse effect on in situ bioremediation.
31472351	13	69	theme	mechanistic	1803:1813	arg1	basis					1815:1819	a mechanistic basis	1801:1819	a mechanistic basis for the regulation of aniline degradation in Rhodococcus sp	1801:1879	This study provides a mechanistic basis for the regulation of aniline degradation in Rhodococcus sp.
31472351	4	70	dep	morphology	416:425	arg1	the					407:409	the	407:409	the	407:409	In this paper, we studied the cell morphology and fatty acid composition of an aniline-degrading strain Rhodococcus sp.
31472351	13	71	from	regulation	1829:1838	arg1	sp					1878:1879	Rhodococcus sp	1866:1879	Rhodococcus sp	1866:1879	This study provides a mechanistic basis for the regulation of aniline degradation in Rhodococcus sp.
31472351	1	72	theme	different	53:61	arg1	gradients					75:83	different temperature gradients	53:83	different temperature gradients: Physiological and transcriptome	53:116	CNS16 under different temperature gradients: Physiological and transcriptome.
31472351	9	73	theme	fatty	1116:1120	arg1	acid					1122:1125	Filamentous Temperature Sensitive as well as unsaturated fatty acid	1059:1125	Filamentous Temperature Sensitive as well as unsaturated fatty acid synthesis	1059:1135	The inhibition of peptidoglycan synthase expression and up-regulation of Filamentous Temperature Sensitive as well as unsaturated fatty acid synthesis genes at suboptimal temperatures might be closely related to corresponding changes in cell morphology and fatty acids composition.
31472351	10	74	theme	thioredoxin-dependent	1353:1373	arg1	peroxidase					1381:1390	thioredoxin-dependent thiol peroxidase	1353:1390	thioredoxin-dependent thiol peroxidase	1353:1390	Strain CNS16 showed loss of catalase and superoxide dismutase activity, and utilized thioredoxin-dependent thiol peroxidase to resist oxidative stress.
31472351	6	75	theme	CNS16	598:602	arg1	morphology					584:593	cell morphology	579:593	cell morphology of CNS16	579:602	At suboptimal temperatures, cell morphology of CNS16 changed from short rod-shaped to long rod or irregular shaped, and the proportion of unsaturated fatty acids was upregulated.
31472351	6	76	theme	acids	707:711	arg1	proportion					675:684	the proportion	671:684	the proportion of unsaturated fatty acids	671:711	At suboptimal temperatures, cell morphology of CNS16 changed from short rod-shaped to long rod or irregular shaped, and the proportion of unsaturated fatty acids was upregulated.
31472351	1	77	theme	Physiological	86:98	arg1	gradients					75:83	different temperature gradients	53:83	different temperature gradients: Physiological and transcriptome	53:116	CNS16 under different temperature gradients: Physiological and transcriptome.
31472351	12	78	theme	environmental	1759:1771	arg1	stress					1773:1778	environmental stress	1759:1778	environmental stress	1759:1778	Amino acid metabolism, coenzyme and vitamin metabolism, ABC transport, and energy metabolism are essential for peptidoglycan synthesis and regulation of cellular metabolism; therefore, synergistically resisting environmental stress.
31472351	10	79	used	utilized	1344:1351	arg2	Strain					1268:1273	Strain CNS16	1268:1279	Strain CNS16	1268:1279	Strain CNS16 showed loss of catalase and superoxide dismutase activity, and utilized thioredoxin-dependent thiol peroxidase to resist oxidative stress.
31472351	6	80	theme	unsaturated	689:699	arg1	acids					707:711	unsaturated fatty acids	689:711	unsaturated fatty acids	689:711	At suboptimal temperatures, cell morphology of CNS16 changed from short rod-shaped to long rod or irregular shaped, and the proportion of unsaturated fatty acids was upregulated.
31472351	0	81	theme	Rhodococcus	25:35	arg1	sp					37:38	Rhodococcus sp	25:38	Rhodococcus sp	25:38	Adaptation mechanisms of Rhodococcus sp.
31472351	7	82	theme	CNS16	835:839	arg1	mechanisms					821:830	the adaptive mechanisms	808:830	the adaptive mechanisms of CNS16 subjected to suboptimal temperatures	808:876	Transcriptomic technologies were then utilized to gain detailed insights into the adaptive mechanisms of CNS16 subjected to suboptimal temperatures.
31472351	1	83	theme	transcriptome	104:116	arg1	gradients					75:83	different temperature gradients	53:83	different temperature gradients: Physiological and transcriptome	53:116	CNS16 under different temperature gradients: Physiological and transcriptome.
31472351	8	84	theme	gene	917:920	arg1	responses					922:930	gene responses	917:930	gene responses	917:930	The results showed that the number of gene responses was significantly higher at 10 °C than that at 20 °C.
31472351	3	85	dep	in	357:358	arg1	situ					360:363	situ	360:363	situ	360:363	However, seasonal changes in ambient temperature, especially rapid temperature drops exert an adverse effect on in situ bioremediation.
31472351	12	86	theme	vitamin	1584:1590	arg1	metabolism					1592:1601	vitamin metabolism	1584:1601	vitamin metabolism	1584:1601	Amino acid metabolism, coenzyme and vitamin metabolism, ABC transport, and energy metabolism are essential for peptidoglycan synthesis and regulation of cellular metabolism; therefore, synergistically resisting environmental stress.
31472351	3	87	from	changes	263:269	arg1	drops					324:328	especially rapid temperature drops	295:328	especially rapid temperature drops	295:328	However, seasonal changes in ambient temperature, especially rapid temperature drops exert an adverse effect on in situ bioremediation.
31472351	3	87	from	changes	263:269	arg1	temperature					282:292	ambient temperature	274:292	ambient temperature	274:292	However, seasonal changes in ambient temperature, especially rapid temperature drops exert an adverse effect on in situ bioremediation.
31472351	12	88	theme	Amino	1548:1552	arg1	metabolism					1559:1568	Amino acid metabolism	1548:1568	Amino acid metabolism	1548:1568	Amino acid metabolism, coenzyme and vitamin metabolism, ABC transport, and energy metabolism are essential for peptidoglycan synthesis and regulation of cellular metabolism; therefore, synergistically resisting environmental stress.
31472351	10	89	theme	superoxide	1309:1318	arg1	dismutase					1320:1328	superoxide dismutase	1309:1328	superoxide dismutase	1309:1328	Strain CNS16 showed loss of catalase and superoxide dismutase activity, and utilized thioredoxin-dependent thiol peroxidase to resist oxidative stress.
31472351	2	90	theme	environmental	215:227	arg1	bioremediation					229:242	environmental bioremediation	215:242	environmental bioremediation	215:242	Rhodococcus exhibits strong adaptability to environmental stressors and plays a crucial role in environmental bioremediation.
31472351	9	91	theme	corresponding	1198:1210	arg1	changes					1212:1218	corresponding changes	1198:1218	corresponding changes in cell morphology and fatty acids composition	1198:1265	The inhibition of peptidoglycan synthase expression and up-regulation of Filamentous Temperature Sensitive as well as unsaturated fatty acid synthesis genes at suboptimal temperatures might be closely related to corresponding changes in cell morphology and fatty acids composition.
31472351	9	92	theme	synthesis	1127:1135	arg1	expression					1027:1036	peptidoglycan synthase expression	1004:1036	peptidoglycan synthase expression	1004:1036	The inhibition of peptidoglycan synthase expression and up-regulation of Filamentous Temperature Sensitive as well as unsaturated fatty acid synthesis genes at suboptimal temperatures might be closely related to corresponding changes in cell morphology and fatty acids composition.
31472351	9	92	theme	synthesis	1127:1135	arg1	up-regulation					1042:1054	up-regulation	1042:1054	up-regulation of Filamentous Temperature Sensitive as well as unsaturated fatty acid synthesis	1042:1135	The inhibition of peptidoglycan synthase expression and up-regulation of Filamentous Temperature Sensitive as well as unsaturated fatty acid synthesis genes at suboptimal temperatures might be closely related to corresponding changes in cell morphology and fatty acids composition.
31472351	7	93	used	utilized	768:775	arg2	technologies					745:756	Transcriptomic technologies	730:756	Transcriptomic technologies	730:756	Transcriptomic technologies were then utilized to gain detailed insights into the adaptive mechanisms of CNS16 subjected to suboptimal temperatures.
30084254	6	0	theme	enzymes	1347:1353	arg1	activities					1308:1317	the low but persistent activities	1285:1317	the low but persistent activities of several starch degrading enzymes present in Cascade hops including amyloglucosidase, α-amylase, β-amylase, and limit dextrinase	1285:1448	The refermentation of beer driven by dry-hopping was attributed to the low but persistent activities of several starch degrading enzymes present in Cascade hops including amyloglucosidase, α-amylase, β-amylase, and limit dextrinase.
30084254	6	1	attach	present	1355:1361	arg1	hops					1374:1377	Cascade hops	1366:1377	Cascade hops including amyloglucosidase, α-amylase, β-amylase, and limit dextrinase	1366:1448	The refermentation of beer driven by dry-hopping was attributed to the low but persistent activities of several starch degrading enzymes present in Cascade hops including amyloglucosidase, α-amylase, β-amylase, and limit dextrinase.
30084254	6	1	attach	present	1355:1361	arg2	enzymes					1347:1353	several starch degrading enzymes	1322:1353	several starch degrading enzymes present in Cascade hops including amyloglucosidase, α-amylase, β-amylase, and limit dextrinase	1322:1448	The refermentation of beer driven by dry-hopping was attributed to the low but persistent activities of several starch degrading enzymes present in Cascade hops including amyloglucosidase, α-amylase, β-amylase, and limit dextrinase.
30084254	4	2	theme	°P	1063:1064	arg1	reduction					1034:1042	a total reduction	1026:1042	a total reduction of approximately 2 °P after 40 days	1026:1078	Fully attenuated and packaged commercial lager beer was dry-hopped at a rate of 10 g hops/L beer with pelletized Cascade hops, dosed with 106 cells/mL of ale yeast, and incubated at 20 °C. Real extract of the treated beer declined significantly within several days with a reduction of 1 °P (% w/w) after 5 days and then slowly to a total reduction of approximately 2 °P after 40 days.
30084254	2	3	theme	active	312:317	arg1	compounds					319:327	biologically active compounds	299:327	biologically active compounds that may also extract into beer and complicate the brewing process by altering the final composition of beer	299:436	Previously assumed to be inert ingredients, recent evidence suggests that hops contain biologically active compounds that may also extract into beer and complicate the brewing process by altering the final composition of beer.
30084254	4	4	theme	°P	983:984	arg1	reduction					968:976	a reduction	966:976	a reduction of 1 °P (% w/w)	966:992	Fully attenuated and packaged commercial lager beer was dry-hopped at a rate of 10 g hops/L beer with pelletized Cascade hops, dosed with 106 cells/mL of ale yeast, and incubated at 20 °C. Real extract of the treated beer declined significantly within several days with a reduction of 1 °P (% w/w) after 5 days and then slowly to a total reduction of approximately 2 °P after 40 days.
30084254	2	5	theme	beer	433:436	arg1	composition					418:428	the final composition	408:428	the final composition of beer	408:436	Previously assumed to be inert ingredients, recent evidence suggests that hops contain biologically active compounds that may also extract into beer and complicate the brewing process by altering the final composition of beer.
30084254	3	6	theme	carbohydrates	662:674	arg1	composition					616:626	the composition	612:626	the composition of fermentable and nonfermentable carbohydrates in dry-hopped beer	612:693	Experiments described herein provide evidence of microbial and/or plant-derived enzymes associated with hops ( Humulus lupulus) which can impact beer quality by influencing the composition of fermentable and nonfermentable carbohydrates in dry-hopped beer.
30084254	8	7	theme	safety	1651:1656	arg1	issues					1658:1663	the unexpected quality and safety issues	1624:1663	issues	1658:1663	Characterizing bioactive enzymes in hops will help hop suppliers and brewers to address the unexpected quality and safety issues surrounding hopping practices in beer.
30084254	6	8	theme	low	1289:1291	arg1	activities					1308:1317	the low but persistent activities	1285:1317	the low but persistent activities of several starch degrading enzymes present in Cascade hops including amyloglucosidase, α-amylase, β-amylase, and limit dextrinase	1285:1448	The refermentation of beer driven by dry-hopping was attributed to the low but persistent activities of several starch degrading enzymes present in Cascade hops including amyloglucosidase, α-amylase, β-amylase, and limit dextrinase.
30084254	0	9	from	Evidence	0:7	arg1	Hops					51:54	Cascade Hops	43:54	Cascade Hops ( Humulus lupulus)	43:73	Evidence of Dextrin Hydrolyzing Enzymes in Cascade Hops ( Humulus lupulus).
30084254	7	10	from	effect	1455:1460	arg1	beer					1488:1491	beer	1488:1491	beer	1488:1491	The effect of hop-derived enzymes on beer was time, temperature, and dose-dependent.
30084254	2	11	theme	inert	237:241	arg1	ingredients					243:253	inert ingredients	237:253	inert ingredients	237:253	Previously assumed to be inert ingredients, recent evidence suggests that hops contain biologically active compounds that may also extract into beer and complicate the brewing process by altering the final composition of beer.
30084254	4	12	theme	%	987:987	arg1	°P					983:984	1 °P	981:984	1 °P (% w/w)	981:992	Fully attenuated and packaged commercial lager beer was dry-hopped at a rate of 10 g hops/L beer with pelletized Cascade hops, dosed with 106 cells/mL of ale yeast, and incubated at 20 °C. Real extract of the treated beer declined significantly within several days with a reduction of 1 °P (% w/w) after 5 days and then slowly to a total reduction of approximately 2 °P after 40 days.
30084254	4	12	theme	%	987:987	arg1	w/w					989:991	% w/w	987:991	% w/w	987:991	Fully attenuated and packaged commercial lager beer was dry-hopped at a rate of 10 g hops/L beer with pelletized Cascade hops, dosed with 106 cells/mL of ale yeast, and incubated at 20 °C. Real extract of the treated beer declined significantly within several days with a reduction of 1 °P (% w/w) after 5 days and then slowly to a total reduction of approximately 2 °P after 40 days.
30084254	4	13	theme	Real	885:888	arg1	extract					890:896	20 °C. Real extract	878:896	20 °C. Real extract of the treated beer declined significantly within several days with a reduction of 1 °P (% w/w) after 5 days and then slowly to a total reduction of approximately 2 °P after 40 days	878:1078	Fully attenuated and packaged commercial lager beer was dry-hopped at a rate of 10 g hops/L beer with pelletized Cascade hops, dosed with 106 cells/mL of ale yeast, and incubated at 20 °C. Real extract of the treated beer declined significantly within several days with a reduction of 1 °P (% w/w) after 5 days and then slowly to a total reduction of approximately 2 °P after 40 days.
30084254	0	14	from	Hops	51:54	arg1	Evidence					0:7	Evidence	0:7	Evidence of Dextrin Hydrolyzing Enzymes in Cascade Hops ( Humulus lupulus).	0:74	Evidence of Dextrin Hydrolyzing Enzymes in Cascade Hops ( Humulus lupulus).
30084254	3	15	theme	enzymes	519:525	arg1	evidence					476:483	evidence	476:483	evidence of microbial and/or plant-derived enzymes associated with hops ( Humulus lupulus) which can impact beer quality by influencing the composition of fermentable and nonfermentable carbohydrates in dry-hopped beer	476:693	Experiments described herein provide evidence of microbial and/or plant-derived enzymes associated with hops ( Humulus lupulus) which can impact beer quality by influencing the composition of fermentable and nonfermentable carbohydrates in dry-hopped beer.
30084254	4	16	theme	total	1028:1032	arg1	reduction					1034:1042	a total reduction	1026:1042	a total reduction of approximately 2 °P after 40 days	1026:1078	Fully attenuated and packaged commercial lager beer was dry-hopped at a rate of 10 g hops/L beer with pelletized Cascade hops, dosed with 106 cells/mL of ale yeast, and incubated at 20 °C. Real extract of the treated beer declined significantly within several days with a reduction of 1 °P (% w/w) after 5 days and then slowly to a total reduction of approximately 2 °P after 40 days.
30084254	6	17	theme	Cascade	1366:1372	arg1	hops					1374:1377	Cascade hops	1366:1377	Cascade hops including amyloglucosidase, α-amylase, β-amylase, and limit dextrinase	1366:1448	The refermentation of beer driven by dry-hopping was attributed to the low but persistent activities of several starch degrading enzymes present in Cascade hops including amyloglucosidase, α-amylase, β-amylase, and limit dextrinase.
30084254	3	18	link	plant-derived	505:517	arg1	enzymes					519:525	microbial and/or plant-derived enzymes	488:525	enzymes	519:525	Experiments described herein provide evidence of microbial and/or plant-derived enzymes associated with hops ( Humulus lupulus) which can impact beer quality by influencing the composition of fermentable and nonfermentable carbohydrates in dry-hopped beer.
30084254	8	19	theme	bioactive	1551:1559	arg1	enzymes					1561:1567	bioactive enzymes	1551:1567	bioactive enzymes	1551:1567	Characterizing bioactive enzymes in hops will help hop suppliers and brewers to address the unexpected quality and safety issues surrounding hopping practices in beer.
30084254	6	20	from	hops	1374:1377	arg1	present					1355:1361	present	1355:1361	present	1355:1361	The refermentation of beer driven by dry-hopping was attributed to the low but persistent activities of several starch degrading enzymes present in Cascade hops including amyloglucosidase, α-amylase, β-amylase, and limit dextrinase.
30084254	3	21	theme	microbial	488:496	arg1	enzymes					519:525	microbial and/or plant-derived enzymes	488:525	enzymes	519:525	Experiments described herein provide evidence of microbial and/or plant-derived enzymes associated with hops ( Humulus lupulus) which can impact beer quality by influencing the composition of fermentable and nonfermentable carbohydrates in dry-hopped beer.
30084254	5	22	theme	additional	1184:1193	arg1	CO2					1173:1175	CO2	1173:1175	CO2	1173:1175	When fully fermented, this was equivalent to the production of an additional 4.75% (v/v) of CO2 and an additional 1.3% (v/v) of alcohol.
30084254	5	22	theme	additional	1184:1193	arg1	v/v					1201:1203	v/v	1201:1203	v/v	1201:1203	When fully fermented, this was equivalent to the production of an additional 4.75% (v/v) of CO2 and an additional 1.3% (v/v) of alcohol.
30084254	5	22	theme	additional	1184:1193	arg1	alcohol					1209:1215	alcohol	1209:1215	alcohol	1209:1215	When fully fermented, this was equivalent to the production of an additional 4.75% (v/v) of CO2 and an additional 1.3% (v/v) of alcohol.
30084254	5	22	theme	additional	1184:1193	arg1	%					1198:1198	an additional 1.3%	1181:1198	an additional 1.3% (v/v) of alcohol	1181:1215	When fully fermented, this was equivalent to the production of an additional 4.75% (v/v) of CO2 and an additional 1.3% (v/v) of alcohol.
30084254	0	23	theme	Cascade	43:49	arg1	Hops					51:54	Cascade Hops	43:54	Cascade Hops ( Humulus lupulus)	43:73	Evidence of Dextrin Hydrolyzing Enzymes in Cascade Hops ( Humulus lupulus).
30084254	4	24	theme	Cascade	809:815	arg1	hops					817:820	pelletized Cascade hops	798:820	pelletized Cascade hops	798:820	Fully attenuated and packaged commercial lager beer was dry-hopped at a rate of 10 g hops/L beer with pelletized Cascade hops, dosed with 106 cells/mL of ale yeast, and incubated at 20 °C. Real extract of the treated beer declined significantly within several days with a reduction of 1 °P (% w/w) after 5 days and then slowly to a total reduction of approximately 2 °P after 40 days.
30084254	6	25	from	present	1355:1361	arg1	hops					1374:1377	Cascade hops	1366:1377	Cascade hops including amyloglucosidase, α-amylase, β-amylase, and limit dextrinase	1366:1448	The refermentation of beer driven by dry-hopping was attributed to the low but persistent activities of several starch degrading enzymes present in Cascade hops including amyloglucosidase, α-amylase, β-amylase, and limit dextrinase.
30084254	3	26	dep	hops	543:546	arg1	lupulus					558:564	Humulus lupulus	550:564	Humulus lupulus	550:564	Experiments described herein provide evidence of microbial and/or plant-derived enzymes associated with hops ( Humulus lupulus) which can impact beer quality by influencing the composition of fermentable and nonfermentable carbohydrates in dry-hopped beer.
30084254	0	27	from	Enzymes	32:38	arg1	Hops					51:54	Cascade Hops	43:54	Cascade Hops ( Humulus lupulus)	43:73	Evidence of Dextrin Hydrolyzing Enzymes in Cascade Hops ( Humulus lupulus).
30084254	4	28	with	days	956:959	arg1	reduction					968:976	a reduction	966:976	a reduction of 1 °P (% w/w)	966:992	Fully attenuated and packaged commercial lager beer was dry-hopped at a rate of 10 g hops/L beer with pelletized Cascade hops, dosed with 106 cells/mL of ale yeast, and incubated at 20 °C. Real extract of the treated beer declined significantly within several days with a reduction of 1 °P (% w/w) after 5 days and then slowly to a total reduction of approximately 2 °P after 40 days.
30084254	4	29	theme	commercial	726:735	arg1	beer					743:746	Fully attenuated and packaged commercial lager beer	696:746	Fully attenuated and packaged commercial lager beer	696:746	Fully attenuated and packaged commercial lager beer was dry-hopped at a rate of 10 g hops/L beer with pelletized Cascade hops, dosed with 106 cells/mL of ale yeast, and incubated at 20 °C. Real extract of the treated beer declined significantly within several days with a reduction of 1 °P (% w/w) after 5 days and then slowly to a total reduction of approximately 2 °P after 40 days.
30084254	5	30	theme	%	1198:1198	arg1	production					1130:1139	the production	1126:1139	the production of an additional 4.75% (v/v) of CO2 and an additional 1.3% (v/v) of alcohol	1126:1215	When fully fermented, this was equivalent to the production of an additional 4.75% (v/v) of CO2 and an additional 1.3% (v/v) of alcohol.
30084254	8	31	theme	hop	1587:1589	arg1	suppliers					1591:1599	hop suppliers	1587:1599	hop suppliers	1587:1599	Characterizing bioactive enzymes in hops will help hop suppliers and brewers to address the unexpected quality and safety issues surrounding hopping practices in beer.
30084254	8	32	theme	unexpected	1628:1637	arg1	quality					1639:1645	the unexpected quality and safety issues	1624:1663	quality	1639:1645	Characterizing bioactive enzymes in hops will help hop suppliers and brewers to address the unexpected quality and safety issues surrounding hopping practices in beer.
30084254	6	33	theme	present	1355:1361	arg1	enzymes					1347:1353	several starch degrading enzymes	1322:1353	several starch degrading enzymes present in Cascade hops including amyloglucosidase, α-amylase, β-amylase, and limit dextrinase	1322:1448	The refermentation of beer driven by dry-hopping was attributed to the low but persistent activities of several starch degrading enzymes present in Cascade hops including amyloglucosidase, α-amylase, β-amylase, and limit dextrinase.
30084254	2	34	theme	recent	256:261	arg1	evidence					263:270	recent evidence	256:270	recent evidence	256:270	Previously assumed to be inert ingredients, recent evidence suggests that hops contain biologically active compounds that may also extract into beer and complicate the brewing process by altering the final composition of beer.
30084254	0	35	theme	Hydrolyzing	20:30	arg1	Enzymes					32:38	Dextrin Hydrolyzing Enzymes	12:38	Dextrin Hydrolyzing Enzymes in Cascade Hops ( Humulus lupulus)	12:73	Evidence of Dextrin Hydrolyzing Enzymes in Cascade Hops ( Humulus lupulus).
30084254	4	36	theme	packaged	717:724	arg1	beer					743:746	Fully attenuated and packaged commercial lager beer	696:746	Fully attenuated and packaged commercial lager beer	696:746	Fully attenuated and packaged commercial lager beer was dry-hopped at a rate of 10 g hops/L beer with pelletized Cascade hops, dosed with 106 cells/mL of ale yeast, and incubated at 20 °C. Real extract of the treated beer declined significantly within several days with a reduction of 1 °P (% w/w) after 5 days and then slowly to a total reduction of approximately 2 °P after 40 days.
30084254	4	37	theme	beer	788:791	arg1	rate					768:771	a rate	766:771	a rate of 10 g hops/L beer	766:791	Fully attenuated and packaged commercial lager beer was dry-hopped at a rate of 10 g hops/L beer with pelletized Cascade hops, dosed with 106 cells/mL of ale yeast, and incubated at 20 °C. Real extract of the treated beer declined significantly within several days with a reduction of 1 °P (% w/w) after 5 days and then slowly to a total reduction of approximately 2 °P after 40 days.
30084254	1	38	theme	common	153:158	arg1	practice					160:167	a common practice	151:167	a common practice in brewing to impart hoppy flavor to beer	151:209	Dry-hopping, the addition of hops to beer during or after fermentation, is a common practice in brewing to impart hoppy flavor to beer.
30084254	1	38	theme	common	153:158	arg1	Dry-hopping					76:86	Dry-hopping	76:86	Dry-hopping	76:86	Dry-hopping, the addition of hops to beer during or after fermentation, is a common practice in brewing to impart hoppy flavor to beer.
30084254	2	39	theme	brewing	380:386	arg1	process					388:394	the brewing process	376:394	the brewing process	376:394	Previously assumed to be inert ingredients, recent evidence suggests that hops contain biologically active compounds that may also extract into beer and complicate the brewing process by altering the final composition of beer.
30084254	7	40	theme	hop-derived	1465:1475	arg1	enzymes					1477:1483	hop-derived enzymes	1465:1483	hop-derived enzymes	1465:1483	The effect of hop-derived enzymes on beer was time, temperature, and dose-dependent.
30084254	4	41	theme	lager	737:741	arg1	beer					743:746	Fully attenuated and packaged commercial lager beer	696:746	Fully attenuated and packaged commercial lager beer	696:746	Fully attenuated and packaged commercial lager beer was dry-hopped at a rate of 10 g hops/L beer with pelletized Cascade hops, dosed with 106 cells/mL of ale yeast, and incubated at 20 °C. Real extract of the treated beer declined significantly within several days with a reduction of 1 °P (% w/w) after 5 days and then slowly to a total reduction of approximately 2 °P after 40 days.
30084254	5	42	theme	CO2	1173:1175	arg1	alcohol					1209:1215	alcohol	1209:1215	alcohol	1209:1215	When fully fermented, this was equivalent to the production of an additional 4.75% (v/v) of CO2 and an additional 1.3% (v/v) of alcohol.
30084254	5	42	theme	CO2	1173:1175	arg1	%					1198:1198	an additional 1.3%	1181:1198	an additional 1.3% (v/v) of alcohol	1181:1215	When fully fermented, this was equivalent to the production of an additional 4.75% (v/v) of CO2 and an additional 1.3% (v/v) of alcohol.
30084254	5	42	theme	CO2	1173:1175	arg1	CO2					1173:1175	CO2	1173:1175	CO2	1173:1175	When fully fermented, this was equivalent to the production of an additional 4.75% (v/v) of CO2 and an additional 1.3% (v/v) of alcohol.
30084254	5	42	theme	CO2	1173:1175	arg1	v/v					1201:1203	v/v	1201:1203	v/v	1201:1203	When fully fermented, this was equivalent to the production of an additional 4.75% (v/v) of CO2 and an additional 1.3% (v/v) of alcohol.
30084254	5	42	theme	CO2	1173:1175	arg1	%					1162:1162	an additional 4.75%	1144:1162	an additional 4.75% (v/v) of CO2	1144:1175	When fully fermented, this was equivalent to the production of an additional 4.75% (v/v) of CO2 and an additional 1.3% (v/v) of alcohol.
30084254	5	42	theme	CO2	1173:1175	arg1	v/v					1165:1167	v/v	1165:1167	v/v	1165:1167	When fully fermented, this was equivalent to the production of an additional 4.75% (v/v) of CO2 and an additional 1.3% (v/v) of alcohol.
30084254	4	43	theme	several	948:954	arg1	days					956:959	several days	948:959	several days with a reduction of 1 °P (% w/w)	948:992	Fully attenuated and packaged commercial lager beer was dry-hopped at a rate of 10 g hops/L beer with pelletized Cascade hops, dosed with 106 cells/mL of ale yeast, and incubated at 20 °C. Real extract of the treated beer declined significantly within several days with a reduction of 1 °P (% w/w) after 5 days and then slowly to a total reduction of approximately 2 °P after 40 days.
30084254	7	44	theme	enzymes	1477:1483	arg1	effect					1455:1460	The effect	1451:1460	The effect of hop-derived enzymes on beer	1451:1491	The effect of hop-derived enzymes on beer was time, temperature, and dose-dependent.
30084254	7	44	theme	enzymes	1477:1483	arg1	time					1497:1500	time	1497:1500	time	1497:1500	The effect of hop-derived enzymes on beer was time, temperature, and dose-dependent.
30084254	4	45	theme	°C.	881:883	arg1	extract					890:896	20 °C. Real extract	878:896	20 °C. Real extract of the treated beer declined significantly within several days with a reduction of 1 °P (% w/w) after 5 days and then slowly to a total reduction of approximately 2 °P after 40 days	878:1078	Fully attenuated and packaged commercial lager beer was dry-hopped at a rate of 10 g hops/L beer with pelletized Cascade hops, dosed with 106 cells/mL of ale yeast, and incubated at 20 °C. Real extract of the treated beer declined significantly within several days with a reduction of 1 °P (% w/w) after 5 days and then slowly to a total reduction of approximately 2 °P after 40 days.
30084254	8	46	theme	hopping	1677:1683	arg1	practices					1685:1693	hopping practices	1677:1693	hopping practices	1677:1693	Characterizing bioactive enzymes in hops will help hop suppliers and brewers to address the unexpected quality and safety issues surrounding hopping practices in beer.
30084254	0	47	dep	Hops	51:54	arg1	lupulus					66:72	Humulus lupulus	58:72	Humulus lupulus	58:72	Evidence of Dextrin Hydrolyzing Enzymes in Cascade Hops ( Humulus lupulus).
30084254	6	48	theme	degrading	1337:1345	arg1	enzymes					1347:1353	several starch degrading enzymes	1322:1353	several starch degrading enzymes present in Cascade hops including amyloglucosidase, α-amylase, β-amylase, and limit dextrinase	1322:1448	The refermentation of beer driven by dry-hopping was attributed to the low but persistent activities of several starch degrading enzymes present in Cascade hops including amyloglucosidase, α-amylase, β-amylase, and limit dextrinase.
30084254	0	49	theme	Enzymes	32:38	arg1	Evidence					0:7	Evidence	0:7	Evidence of Dextrin Hydrolyzing Enzymes in Cascade Hops ( Humulus lupulus).	0:74	Evidence of Dextrin Hydrolyzing Enzymes in Cascade Hops ( Humulus lupulus).
30084254	4	50	theme	20	878:879	arg1	°C.					881:883	°C.	881:883	°C.	881:883	Fully attenuated and packaged commercial lager beer was dry-hopped at a rate of 10 g hops/L beer with pelletized Cascade hops, dosed with 106 cells/mL of ale yeast, and incubated at 20 °C. Real extract of the treated beer declined significantly within several days with a reduction of 1 °P (% w/w) after 5 days and then slowly to a total reduction of approximately 2 °P after 40 days.
30084254	4	51	theme	hops/L	781:786	arg1	beer					788:791	10 g hops/L beer	776:791	10 g hops/L beer	776:791	Fully attenuated and packaged commercial lager beer was dry-hopped at a rate of 10 g hops/L beer with pelletized Cascade hops, dosed with 106 cells/mL of ale yeast, and incubated at 20 °C. Real extract of the treated beer declined significantly within several days with a reduction of 1 °P (% w/w) after 5 days and then slowly to a total reduction of approximately 2 °P after 40 days.
30084254	5	52	theme	alcohol	1209:1215	arg1	alcohol					1209:1215	alcohol	1209:1215	alcohol	1209:1215	When fully fermented, this was equivalent to the production of an additional 4.75% (v/v) of CO2 and an additional 1.3% (v/v) of alcohol.
30084254	5	52	theme	alcohol	1209:1215	arg1	%					1198:1198	an additional 1.3%	1181:1198	an additional 1.3% (v/v) of alcohol	1181:1215	When fully fermented, this was equivalent to the production of an additional 4.75% (v/v) of CO2 and an additional 1.3% (v/v) of alcohol.
30084254	5	52	theme	alcohol	1209:1215	arg1	CO2					1173:1175	CO2	1173:1175	CO2	1173:1175	When fully fermented, this was equivalent to the production of an additional 4.75% (v/v) of CO2 and an additional 1.3% (v/v) of alcohol.
30084254	5	52	theme	alcohol	1209:1215	arg1	v/v					1201:1203	v/v	1201:1203	v/v	1201:1203	When fully fermented, this was equivalent to the production of an additional 4.75% (v/v) of CO2 and an additional 1.3% (v/v) of alcohol.
30084254	5	52	theme	alcohol	1209:1215	arg1	%					1162:1162	an additional 4.75%	1144:1162	an additional 4.75% (v/v) of CO2	1144:1175	When fully fermented, this was equivalent to the production of an additional 4.75% (v/v) of CO2 and an additional 1.3% (v/v) of alcohol.
30084254	5	52	theme	alcohol	1209:1215	arg1	v/v					1165:1167	v/v	1165:1167	v/v	1165:1167	When fully fermented, this was equivalent to the production of an additional 4.75% (v/v) of CO2 and an additional 1.3% (v/v) of alcohol.
30084254	3	53	from	composition	616:626	arg1	beer					690:693	dry-hopped beer	679:693	dry-hopped beer	679:693	Experiments described herein provide evidence of microbial and/or plant-derived enzymes associated with hops ( Humulus lupulus) which can impact beer quality by influencing the composition of fermentable and nonfermentable carbohydrates in dry-hopped beer.
30084254	2	54	theme	final	412:416	arg1	composition					418:428	the final composition	408:428	the final composition of beer	408:436	Previously assumed to be inert ingredients, recent evidence suggests that hops contain biologically active compounds that may also extract into beer and complicate the brewing process by altering the final composition of beer.
30084254	5	55	theme	additional	1147:1156	arg1	CO2					1173:1175	CO2	1173:1175	CO2	1173:1175	When fully fermented, this was equivalent to the production of an additional 4.75% (v/v) of CO2 and an additional 1.3% (v/v) of alcohol.
30084254	5	55	theme	additional	1147:1156	arg1	%					1162:1162	an additional 4.75%	1144:1162	an additional 4.75% (v/v) of CO2	1144:1175	When fully fermented, this was equivalent to the production of an additional 4.75% (v/v) of CO2 and an additional 1.3% (v/v) of alcohol.
30084254	5	55	theme	additional	1147:1156	arg1	alcohol					1209:1215	alcohol	1209:1215	alcohol	1209:1215	When fully fermented, this was equivalent to the production of an additional 4.75% (v/v) of CO2 and an additional 1.3% (v/v) of alcohol.
30084254	5	55	theme	additional	1147:1156	arg1	v/v					1165:1167	v/v	1165:1167	v/v	1165:1167	When fully fermented, this was equivalent to the production of an additional 4.75% (v/v) of CO2 and an additional 1.3% (v/v) of alcohol.
30084254	4	56	theme	yeast	854:858	arg1	cells/mL					838:845	106 cells/mL	834:845	106 cells/mL of ale yeast	834:858	Fully attenuated and packaged commercial lager beer was dry-hopped at a rate of 10 g hops/L beer with pelletized Cascade hops, dosed with 106 cells/mL of ale yeast, and incubated at 20 °C. Real extract of the treated beer declined significantly within several days with a reduction of 1 °P (% w/w) after 5 days and then slowly to a total reduction of approximately 2 °P after 40 days.
30084254	4	57	theme	pelletized	798:807	arg1	hops					817:820	pelletized Cascade hops	798:820	pelletized Cascade hops	798:820	Fully attenuated and packaged commercial lager beer was dry-hopped at a rate of 10 g hops/L beer with pelletized Cascade hops, dosed with 106 cells/mL of ale yeast, and incubated at 20 °C. Real extract of the treated beer declined significantly within several days with a reduction of 1 °P (% w/w) after 5 days and then slowly to a total reduction of approximately 2 °P after 40 days.
30084254	6	58	theme	limit	1433:1437	arg1	dextrinase					1439:1448	limit dextrinase	1433:1448	limit dextrinase	1433:1448	The refermentation of beer driven by dry-hopping was attributed to the low but persistent activities of several starch degrading enzymes present in Cascade hops including amyloglucosidase, α-amylase, β-amylase, and limit dextrinase.
30084254	2	59	contain	contain	291:297	arg1	hops					286:289	hops	286:289	hops	286:289	Previously assumed to be inert ingredients, recent evidence suggests that hops contain biologically active compounds that may also extract into beer and complicate the brewing process by altering the final composition of beer.
30084254	2	59	contain	contain	291:297	arg2	compounds					319:327	biologically active compounds	299:327	biologically active compounds that may also extract into beer and complicate the brewing process by altering the final composition of beer	299:436	Previously assumed to be inert ingredients, recent evidence suggests that hops contain biologically active compounds that may also extract into beer and complicate the brewing process by altering the final composition of beer.
30084254	6	60	theme	beer	1240:1243	arg1	refermentation					1222:1235	The refermentation	1218:1235	The refermentation of beer driven by dry-hopping	1218:1265	The refermentation of beer driven by dry-hopping was attributed to the low but persistent activities of several starch degrading enzymes present in Cascade hops including amyloglucosidase, α-amylase, β-amylase, and limit dextrinase.
30084254	4	61	theme	ale	850:852	arg1	yeast					854:858	ale yeast	850:858	ale yeast	850:858	Fully attenuated and packaged commercial lager beer was dry-hopped at a rate of 10 g hops/L beer with pelletized Cascade hops, dosed with 106 cells/mL of ale yeast, and incubated at 20 °C. Real extract of the treated beer declined significantly within several days with a reduction of 1 °P (% w/w) after 5 days and then slowly to a total reduction of approximately 2 °P after 40 days.
30084254	6	62	theme	starch	1330:1335	arg1	enzymes					1347:1353	several starch degrading enzymes	1322:1353	several starch degrading enzymes present in Cascade hops including amyloglucosidase, α-amylase, β-amylase, and limit dextrinase	1322:1448	The refermentation of beer driven by dry-hopping was attributed to the low but persistent activities of several starch degrading enzymes present in Cascade hops including amyloglucosidase, α-amylase, β-amylase, and limit dextrinase.
30084254	4	63	dep	beer	913:916	arg1	declined					918:925	declined	918:925	declined significantly within several days with a reduction of 1 °P (% w/w) after 5 days	918:1005	Fully attenuated and packaged commercial lager beer was dry-hopped at a rate of 10 g hops/L beer with pelletized Cascade hops, dosed with 106 cells/mL of ale yeast, and incubated at 20 °C. Real extract of the treated beer declined significantly within several days with a reduction of 1 °P (% w/w) after 5 days and then slowly to a total reduction of approximately 2 °P after 40 days.
30084254	3	64	theme	nonfermentable	647:660	arg1	carbohydrates					662:674	fermentable and nonfermentable carbohydrates	631:674	fermentable and nonfermentable carbohydrates	631:674	Experiments described herein provide evidence of microbial and/or plant-derived enzymes associated with hops ( Humulus lupulus) which can impact beer quality by influencing the composition of fermentable and nonfermentable carbohydrates in dry-hopped beer.
30084254	3	65	theme	beer	584:587	arg1	quality					589:595	beer quality	584:595	beer quality	584:595	Experiments described herein provide evidence of microbial and/or plant-derived enzymes associated with hops ( Humulus lupulus) which can impact beer quality by influencing the composition of fermentable and nonfermentable carbohydrates in dry-hopped beer.
30084254	1	66	theme	hops	105:108	arg1	Dry-hopping					76:86	Dry-hopping	76:86	Dry-hopping	76:86	Dry-hopping, the addition of hops to beer during or after fermentation, is a common practice in brewing to impart hoppy flavor to beer.
30084254	1	66	theme	hops	105:108	arg1	addition					93:100	the addition	89:100	the addition of hops to beer during or after fermentation	89:145	Dry-hopping, the addition of hops to beer during or after fermentation, is a common practice in brewing to impart hoppy flavor to beer.
30084254	5	67	theme	%	1162:1162	arg1	production					1130:1139	the production	1126:1139	the production of an additional 4.75% (v/v) of CO2 and an additional 1.3% (v/v) of alcohol	1126:1215	When fully fermented, this was equivalent to the production of an additional 4.75% (v/v) of CO2 and an additional 1.3% (v/v) of alcohol.
30084254	3	68	theme	plant-derived	505:517	arg1	enzymes					519:525	microbial and/or plant-derived enzymes	488:525	enzymes	519:525	Experiments described herein provide evidence of microbial and/or plant-derived enzymes associated with hops ( Humulus lupulus) which can impact beer quality by influencing the composition of fermentable and nonfermentable carbohydrates in dry-hopped beer.
30084254	7	69	link	hop-derived	1465:1475	arg1	enzymes					1477:1483	hop-derived enzymes	1465:1483	hop-derived enzymes	1465:1483	The effect of hop-derived enzymes on beer was time, temperature, and dose-dependent.
30084254	4	70	theme	attenuated	702:711	arg1	beer					743:746	Fully attenuated and packaged commercial lager beer	696:746	Fully attenuated and packaged commercial lager beer	696:746	Fully attenuated and packaged commercial lager beer was dry-hopped at a rate of 10 g hops/L beer with pelletized Cascade hops, dosed with 106 cells/mL of ale yeast, and incubated at 20 °C. Real extract of the treated beer declined significantly within several days with a reduction of 1 °P (% w/w) after 5 days and then slowly to a total reduction of approximately 2 °P after 40 days.
30084254	4	71	theme	beer	913:916	arg1	extract					890:896	20 °C. Real extract	878:896	20 °C. Real extract of the treated beer declined significantly within several days with a reduction of 1 °P (% w/w) after 5 days and then slowly to a total reduction of approximately 2 °P after 40 days	878:1078	Fully attenuated and packaged commercial lager beer was dry-hopped at a rate of 10 g hops/L beer with pelletized Cascade hops, dosed with 106 cells/mL of ale yeast, and incubated at 20 °C. Real extract of the treated beer declined significantly within several days with a reduction of 1 °P (% w/w) after 5 days and then slowly to a total reduction of approximately 2 °P after 40 days.
30084254	3	72	theme	fermentable	631:641	arg1	carbohydrates					662:674	fermentable and nonfermentable carbohydrates	631:674	fermentable and nonfermentable carbohydrates	631:674	Experiments described herein provide evidence of microbial and/or plant-derived enzymes associated with hops ( Humulus lupulus) which can impact beer quality by influencing the composition of fermentable and nonfermentable carbohydrates in dry-hopped beer.
30084254	4	73	theme	g	779:779	arg1	beer					788:791	10 g hops/L beer	776:791	10 g hops/L beer	776:791	Fully attenuated and packaged commercial lager beer was dry-hopped at a rate of 10 g hops/L beer with pelletized Cascade hops, dosed with 106 cells/mL of ale yeast, and incubated at 20 °C. Real extract of the treated beer declined significantly within several days with a reduction of 1 °P (% w/w) after 5 days and then slowly to a total reduction of approximately 2 °P after 40 days.
30084254	6	74	theme	several	1322:1328	arg1	enzymes					1347:1353	several starch degrading enzymes	1322:1353	several starch degrading enzymes present in Cascade hops including amyloglucosidase, α-amylase, β-amylase, and limit dextrinase	1322:1448	The refermentation of beer driven by dry-hopping was attributed to the low but persistent activities of several starch degrading enzymes present in Cascade hops including amyloglucosidase, α-amylase, β-amylase, and limit dextrinase.
30084254	1	75	theme	hoppy	190:194	arg1	flavor					196:201	hoppy flavor	190:201	hoppy flavor to beer	190:209	Dry-hopping, the addition of hops to beer during or after fermentation, is a common practice in brewing to impart hoppy flavor to beer.
30084254	4	76	theme	treated	905:911	arg1	beer					913:916	the treated beer	901:916	the treated beer declined significantly within several days with a reduction of 1 °P (% w/w) after 5 days and then slowly to a total reduction of approximately 2 °P after 40 days	901:1078	Fully attenuated and packaged commercial lager beer was dry-hopped at a rate of 10 g hops/L beer with pelletized Cascade hops, dosed with 106 cells/mL of ale yeast, and incubated at 20 °C. Real extract of the treated beer declined significantly within several days with a reduction of 1 °P (% w/w) after 5 days and then slowly to a total reduction of approximately 2 °P after 40 days.
30084254	3	77	theme	dry-hopped	679:688	arg1	beer					690:693	dry-hopped beer	679:693	dry-hopped beer	679:693	Experiments described herein provide evidence of microbial and/or plant-derived enzymes associated with hops ( Humulus lupulus) which can impact beer quality by influencing the composition of fermentable and nonfermentable carbohydrates in dry-hopped beer.
30084254	0	78	theme	Dextrin	12:18	arg1	Enzymes					32:38	Dextrin Hydrolyzing Enzymes	12:38	Dextrin Hydrolyzing Enzymes in Cascade Hops ( Humulus lupulus)	12:73	Evidence of Dextrin Hydrolyzing Enzymes in Cascade Hops ( Humulus lupulus).
30084254	6	79	theme	persistent	1297:1306	arg1	activities					1308:1317	the low but persistent activities	1285:1317	the low but persistent activities of several starch degrading enzymes present in Cascade hops including amyloglucosidase, α-amylase, β-amylase, and limit dextrinase	1285:1448	The refermentation of beer driven by dry-hopping was attributed to the low but persistent activities of several starch degrading enzymes present in Cascade hops including amyloglucosidase, α-amylase, β-amylase, and limit dextrinase.
30946907	8	0	theme	Micro	1134:1138	arg1	Tomography					1149:1158	Micro Computed Tomography	1134:1158	Micro Computed Tomography analysis	1134:1167	Micro Computed Tomography analysis suggested that more new tissue was formed in the defects treated with the hydrogels.
30946907	6	1	theme	gel-forming	989:999	arg1	system					1001:1006	a safe in situ gel-forming system	974:1006	a safe in situ gel-forming system	974:1006	The results indicated that the prepared hydrogels were nontoxic upon subcutaneous injection, and could be candidates for a safe in situ gel-forming system.
30946907	4	2	from	increase	676:683	arg1	viability					693:701	cell viability	688:701	cell viability	688:701	Crystal violet assays showed a 27% increase in cell viability when these cells were seeded in hydrogels containing nBG.
30946907	6	3	theme	in	981:982	arg1	system					1001:1006	a safe in situ gel-forming system	974:1006	a safe in situ gel-forming system	974:1006	The results indicated that the prepared hydrogels were nontoxic upon subcutaneous injection, and could be candidates for a safe in situ gel-forming system.
30946907	0	4	theme	In	101:102	arg1	analyses					122:129	In vitro and in vivo analyses	101:129	Injectable chitosan/gelatin/bioactive glass nanocomposite hydrogels for potential bone regeneration: In vitro and in vivo analyses.	0:130	Injectable chitosan/gelatin/bioactive glass nanocomposite hydrogels for potential bone regeneration: In vitro and in vivo analyses.
30946907	6	5	dep	in	981:982	arg1	situ					984:987	situ	984:987	situ	984:987	The results indicated that the prepared hydrogels were nontoxic upon subcutaneous injection, and could be candidates for a safe in situ gel-forming system.
30946907	7	6	theme	regenerative	1110:1121	arg1	potential					1123:1131	the hydrogels' regenerative potential	1095:1131	the hydrogels' regenerative potential	1095:1131	Injection of the hydrogels into a rat tibial defect allowed preliminary evaluation of the hydrogels' regenerative potential.
30946907	6	7	theme	safe	976:979	arg1	system					1001:1006	a safe in situ gel-forming system	974:1006	a safe in situ gel-forming system	974:1006	The results indicated that the prepared hydrogels were nontoxic upon subcutaneous injection, and could be candidates for a safe in situ gel-forming system.
30946907	0	8	theme	in	114:115	arg1	analyses					122:129	In vitro and in vivo analyses	101:129	Injectable chitosan/gelatin/bioactive glass nanocomposite hydrogels for potential bone regeneration: In vitro and in vivo analyses.	0:130	Injectable chitosan/gelatin/bioactive glass nanocomposite hydrogels for potential bone regeneration: In vitro and in vivo analyses.
30946907	6	9	theme	prepared	884:891	arg1	hydrogels					893:901	the prepared hydrogels	880:901	the prepared hydrogels	880:901	The results indicated that the prepared hydrogels were nontoxic upon subcutaneous injection, and could be candidates for a safe in situ gel-forming system.
30946907	6	9	theme	prepared	884:891	arg1	nontoxic					908:915	nontoxic	908:915	nontoxic	908:915	The results indicated that the prepared hydrogels were nontoxic upon subcutaneous injection, and could be candidates for a safe in situ gel-forming system.
30946907	3	10	theme	encapsulated	581:592	arg1	BMSC					634:637	BMSC	634:637	BMSC	634:637	In vitro assays demonstrated the cytocompatibility of the hydrogels with encapsulated rat bone marrow mesenchymal stem cells (BMSC).
30946907	3	10	theme	encapsulated	581:592	arg1	cells					627:631	encapsulated rat bone marrow mesenchymal stem cells	581:631	encapsulated rat bone marrow mesenchymal stem cells (BMSC)	581:638	In vitro assays demonstrated the cytocompatibility of the hydrogels with encapsulated rat bone marrow mesenchymal stem cells (BMSC).
30946907	8	11	theme	Computed	1140:1147	arg1	Tomography					1149:1158	Micro Computed Tomography	1134:1158	Micro Computed Tomography analysis	1134:1167	Micro Computed Tomography analysis suggested that more new tissue was formed in the defects treated with the hydrogels.
30946907	2	12	theme	body	303:306	arg1	fluid					308:312	simulated body fluid	293:312	simulated body fluid	293:312	Assays in SBF (simulated body fluid) solution showed that loss of hydrogel mass in vitro was decreased by 4.3% when bioactive glass nanoparticles (nBG) were incorporated, and confirmed the bioactivity of nBG containing hydrogels.
30946907	2	12	theme	body	303:306	arg1	SBF					288:290	SBF	288:290	SBF (simulated body fluid) solution	288:322	Assays in SBF (simulated body fluid) solution showed that loss of hydrogel mass in vitro was decreased by 4.3% when bioactive glass nanoparticles (nBG) were incorporated, and confirmed the bioactivity of nBG containing hydrogels.
30946907	5	13	theme	Swiss	841:845	arg1	rats					847:850	Swiss rats	841:850	Swiss rats	841:850	In vivo biocompatibility was examined by injecting hydrogels into the dorsum of Swiss rats.
30946907	7	14	theme	preliminary	1069:1079	arg1	evaluation					1081:1090	preliminary evaluation	1069:1090	preliminary evaluation of the hydrogels' regenerative potential	1069:1131	Injection of the hydrogels into a rat tibial defect allowed preliminary evaluation of the hydrogels' regenerative potential.
30946907	0	15	dep	in	114:115	arg1	vivo					117:120	vivo	117:120	vivo	117:120	Injectable chitosan/gelatin/bioactive glass nanocomposite hydrogels for potential bone regeneration: In vitro and in vivo analyses.
30946907	8	16	theme	new	1189:1191	arg1	tissue					1193:1198	more new tissue	1184:1198	more new tissue	1184:1198	Micro Computed Tomography analysis suggested that more new tissue was formed in the defects treated with the hydrogels.
30946907	1	17	theme	in	172:173	arg1	behaviors					180:188	in vitro and in vivo behaviors	159:188	in vitro and in vivo behaviors of thermosensitive composite hydrogels based on polymers/bioactive glass nanoparticles	159:275	The present work describes in vitro and in vivo behaviors of thermosensitive composite hydrogels based on polymers/bioactive glass nanoparticles.
30946907	3	18	with	cytocompatibility	541:557	arg1	BMSC					634:637	BMSC	634:637	BMSC	634:637	In vitro assays demonstrated the cytocompatibility of the hydrogels with encapsulated rat bone marrow mesenchymal stem cells (BMSC).
30946907	3	18	with	cytocompatibility	541:557	arg1	cells					627:631	encapsulated rat bone marrow mesenchymal stem cells	581:631	encapsulated rat bone marrow mesenchymal stem cells (BMSC)	581:638	In vitro assays demonstrated the cytocompatibility of the hydrogels with encapsulated rat bone marrow mesenchymal stem cells (BMSC).
30946907	9	19	theme	developed	1296:1304	arg1	hydrogels					1327:1335	the developed injectable composite hydrogels	1292:1335	the developed injectable composite hydrogels	1292:1335	Taken together, our data suggest that the developed injectable composite hydrogels possess properties which make them suitable candidates for use as temporary injectable matrices for bone regeneration.
30946907	9	20	theme	temporary	1403:1411	arg1	matrices					1424:1431	temporary injectable matrices	1403:1431	temporary injectable matrices for bone regeneration	1403:1453	Taken together, our data suggest that the developed injectable composite hydrogels possess properties which make them suitable candidates for use as temporary injectable matrices for bone regeneration.
30946907	7	21	theme	tibial	1047:1052	arg1	defect					1054:1059	a rat tibial defect	1041:1059	a rat tibial defect	1041:1059	Injection of the hydrogels into a rat tibial defect allowed preliminary evaluation of the hydrogels' regenerative potential.
30946907	4	22	theme	violet	649:654	arg1	assays					656:661	Crystal violet assays	641:661	Crystal violet assays	641:661	Crystal violet assays showed a 27% increase in cell viability when these cells were seeded in hydrogels containing nBG.
30946907	0	23	theme	chitosan/gelatin/bioactive	11:36	arg1	hydrogels					58:66	Injectable chitosan/gelatin/bioactive glass nanocomposite hydrogels	0:66	Injectable chitosan/gelatin/bioactive glass nanocomposite hydrogels for potential bone regeneration: In vitro and in vivo analyses.	0:130	Injectable chitosan/gelatin/bioactive glass nanocomposite hydrogels for potential bone regeneration: In vitro and in vivo analyses.
30946907	9	24	theme	injectable	1306:1315	arg1	hydrogels					1327:1335	the developed injectable composite hydrogels	1292:1335	the developed injectable composite hydrogels	1292:1335	Taken together, our data suggest that the developed injectable composite hydrogels possess properties which make them suitable candidates for use as temporary injectable matrices for bone regeneration.
30946907	5	25	dep	In	761:762	arg1	vivo					764:767	vivo	764:767	vivo	764:767	In vivo biocompatibility was examined by injecting hydrogels into the dorsum of Swiss rats.
30946907	3	26	theme	hydrogels	566:574	arg1	cytocompatibility					541:557	the cytocompatibility	537:557	the cytocompatibility of the hydrogels with encapsulated rat bone marrow mesenchymal stem cells (BMSC)	537:638	In vitro assays demonstrated the cytocompatibility of the hydrogels with encapsulated rat bone marrow mesenchymal stem cells (BMSC).
30946907	4	27	theme	Crystal	641:647	arg1	assays					656:661	Crystal violet assays	641:661	Crystal violet assays	641:661	Crystal violet assays showed a 27% increase in cell viability when these cells were seeded in hydrogels containing nBG.
30946907	0	28	theme	Injectable	0:9	arg1	hydrogels					58:66	Injectable chitosan/gelatin/bioactive glass nanocomposite hydrogels	0:66	Injectable chitosan/gelatin/bioactive glass nanocomposite hydrogels for potential bone regeneration: In vitro and in vivo analyses.	0:130	Injectable chitosan/gelatin/bioactive glass nanocomposite hydrogels for potential bone regeneration: In vitro and in vivo analyses.
30946907	9	29	theme	composite	1317:1325	arg1	hydrogels					1327:1335	the developed injectable composite hydrogels	1292:1335	the developed injectable composite hydrogels	1292:1335	Taken together, our data suggest that the developed injectable composite hydrogels possess properties which make them suitable candidates for use as temporary injectable matrices for bone regeneration.
30946907	0	30	dep	In	101:102	arg1	vitro					104:108	vitro	104:108	vitro	104:108	Injectable chitosan/gelatin/bioactive glass nanocomposite hydrogels for potential bone regeneration: In vitro and in vivo analyses.
30946907	2	31	from	Assays	278:283	arg1	solution					315:322	SBF (simulated body fluid) solution	288:322	SBF (simulated body fluid) solution	288:322	Assays in SBF (simulated body fluid) solution showed that loss of hydrogel mass in vitro was decreased by 4.3% when bioactive glass nanoparticles (nBG) were incorporated, and confirmed the bioactivity of nBG containing hydrogels.
30946907	0	32	theme	nanocomposite	44:56	arg1	hydrogels					58:66	Injectable chitosan/gelatin/bioactive glass nanocomposite hydrogels	0:66	Injectable chitosan/gelatin/bioactive glass nanocomposite hydrogels for potential bone regeneration: In vitro and in vivo analyses.	0:130	Injectable chitosan/gelatin/bioactive glass nanocomposite hydrogels for potential bone regeneration: In vitro and in vivo analyses.
30946907	7	33	theme	rat	1043:1045	arg1	defect					1054:1059	a rat tibial defect	1041:1059	a rat tibial defect	1041:1059	Injection of the hydrogels into a rat tibial defect allowed preliminary evaluation of the hydrogels' regenerative potential.
30946907	3	34	theme	rat	594:596	arg1	BMSC					634:637	BMSC	634:637	BMSC	634:637	In vitro assays demonstrated the cytocompatibility of the hydrogels with encapsulated rat bone marrow mesenchymal stem cells (BMSC).
30946907	3	34	theme	rat	594:596	arg1	cells					627:631	encapsulated rat bone marrow mesenchymal stem cells	581:631	encapsulated rat bone marrow mesenchymal stem cells (BMSC)	581:638	In vitro assays demonstrated the cytocompatibility of the hydrogels with encapsulated rat bone marrow mesenchymal stem cells (BMSC).
30946907	3	35	dep	In	508:509	arg1	vitro					511:515	vitro	511:515	vitro	511:515	In vitro assays demonstrated the cytocompatibility of the hydrogels with encapsulated rat bone marrow mesenchymal stem cells (BMSC).
30946907	9	36	theme	bone	1437:1440	arg1	regeneration					1442:1453	bone regeneration	1437:1453	bone regeneration	1437:1453	Taken together, our data suggest that the developed injectable composite hydrogels possess properties which make them suitable candidates for use as temporary injectable matrices for bone regeneration.
30946907	1	37	theme	thermosensitive	193:207	arg1	hydrogels					219:227	thermosensitive composite hydrogels	193:227	thermosensitive composite hydrogels based on polymers/bioactive glass nanoparticles	193:275	The present work describes in vitro and in vivo behaviors of thermosensitive composite hydrogels based on polymers/bioactive glass nanoparticles.
30946907	0	38	theme	glass	38:42	arg1	hydrogels					58:66	Injectable chitosan/gelatin/bioactive glass nanocomposite hydrogels	0:66	Injectable chitosan/gelatin/bioactive glass nanocomposite hydrogels for potential bone regeneration: In vitro and in vivo analyses.	0:130	Injectable chitosan/gelatin/bioactive glass nanocomposite hydrogels for potential bone regeneration: In vitro and in vivo analyses.
30946907	2	39	theme	simulated	293:301	arg1	fluid					308:312	simulated body fluid	293:312	simulated body fluid	293:312	Assays in SBF (simulated body fluid) solution showed that loss of hydrogel mass in vitro was decreased by 4.3% when bioactive glass nanoparticles (nBG) were incorporated, and confirmed the bioactivity of nBG containing hydrogels.
30946907	2	39	theme	simulated	293:301	arg1	SBF					288:290	SBF	288:290	SBF (simulated body fluid) solution	288:322	Assays in SBF (simulated body fluid) solution showed that loss of hydrogel mass in vitro was decreased by 4.3% when bioactive glass nanoparticles (nBG) were incorporated, and confirmed the bioactivity of nBG containing hydrogels.
30946907	1	40	dep	in	159:160	arg1	vitro					162:166	vitro	162:166	vitro	162:166	The present work describes in vitro and in vivo behaviors of thermosensitive composite hydrogels based on polymers/bioactive glass nanoparticles.
30946907	1	41	theme	composite	209:217	arg1	hydrogels					219:227	thermosensitive composite hydrogels	193:227	thermosensitive composite hydrogels based on polymers/bioactive glass nanoparticles	193:275	The present work describes in vitro and in vivo behaviors of thermosensitive composite hydrogels based on polymers/bioactive glass nanoparticles.
30946907	5	42	theme	In	761:762	arg1	biocompatibility					769:784	In vivo biocompatibility	761:784	In vivo biocompatibility	761:784	In vivo biocompatibility was examined by injecting hydrogels into the dorsum of Swiss rats.
30946907	4	43	theme	cell	688:691	arg1	viability					693:701	cell viability	688:701	cell viability	688:701	Crystal violet assays showed a 27% increase in cell viability when these cells were seeded in hydrogels containing nBG.
30946907	2	44	theme	glass	404:408	arg1	nBG					425:427	nBG	425:427	nBG	425:427	Assays in SBF (simulated body fluid) solution showed that loss of hydrogel mass in vitro was decreased by 4.3% when bioactive glass nanoparticles (nBG) were incorporated, and confirmed the bioactivity of nBG containing hydrogels.
30946907	2	44	theme	glass	404:408	arg1	nanoparticles					410:422	bioactive glass nanoparticles	394:422	bioactive glass nanoparticles (nBG)	394:428	Assays in SBF (simulated body fluid) solution showed that loss of hydrogel mass in vitro was decreased by 4.3% when bioactive glass nanoparticles (nBG) were incorporated, and confirmed the bioactivity of nBG containing hydrogels.
30946907	3	45	theme	bone	598:601	arg1	BMSC					634:637	BMSC	634:637	BMSC	634:637	In vitro assays demonstrated the cytocompatibility of the hydrogels with encapsulated rat bone marrow mesenchymal stem cells (BMSC).
30946907	3	45	theme	bone	598:601	arg1	cells					627:631	encapsulated rat bone marrow mesenchymal stem cells	581:631	encapsulated rat bone marrow mesenchymal stem cells (BMSC)	581:638	In vitro assays demonstrated the cytocompatibility of the hydrogels with encapsulated rat bone marrow mesenchymal stem cells (BMSC).
30946907	1	46	theme	hydrogels	219:227	arg1	behaviors					180:188	in vitro and in vivo behaviors	159:188	in vitro and in vivo behaviors of thermosensitive composite hydrogels based on polymers/bioactive glass nanoparticles	159:275	The present work describes in vitro and in vivo behaviors of thermosensitive composite hydrogels based on polymers/bioactive glass nanoparticles.
30946907	4	47	theme	%	674:674	arg1	increase					676:683	a 27% increase	670:683	a 27% increase in cell viability	670:701	Crystal violet assays showed a 27% increase in cell viability when these cells were seeded in hydrogels containing nBG.
30946907	9	48	theme	suitable	1372:1379	arg1	candidates					1381:1390	suitable candidates	1372:1390	suitable candidates for use as temporary injectable matrices for bone regeneration	1372:1453	Taken together, our data suggest that the developed injectable composite hydrogels possess properties which make them suitable candidates for use as temporary injectable matrices for bone regeneration.
30946907	3	49	theme	marrow	603:608	arg1	BMSC					634:637	BMSC	634:637	BMSC	634:637	In vitro assays demonstrated the cytocompatibility of the hydrogels with encapsulated rat bone marrow mesenchymal stem cells (BMSC).
30946907	3	49	theme	marrow	603:608	arg1	cells					627:631	encapsulated rat bone marrow mesenchymal stem cells	581:631	encapsulated rat bone marrow mesenchymal stem cells (BMSC)	581:638	In vitro assays demonstrated the cytocompatibility of the hydrogels with encapsulated rat bone marrow mesenchymal stem cells (BMSC).
30946907	2	50	theme	bioactive	394:402	arg1	nBG					425:427	nBG	425:427	nBG	425:427	Assays in SBF (simulated body fluid) solution showed that loss of hydrogel mass in vitro was decreased by 4.3% when bioactive glass nanoparticles (nBG) were incorporated, and confirmed the bioactivity of nBG containing hydrogels.
30946907	2	50	theme	bioactive	394:402	arg1	nanoparticles					410:422	bioactive glass nanoparticles	394:422	bioactive glass nanoparticles (nBG)	394:428	Assays in SBF (simulated body fluid) solution showed that loss of hydrogel mass in vitro was decreased by 4.3% when bioactive glass nanoparticles (nBG) were incorporated, and confirmed the bioactivity of nBG containing hydrogels.
30946907	0	51	theme	bone	82:85	arg1	regeneration					87:98	potential bone regeneration	72:98	potential bone regeneration	72:98	Injectable chitosan/gelatin/bioactive glass nanocomposite hydrogels for potential bone regeneration: In vitro and in vivo analyses.
30946907	8	52	theme	Tomography	1149:1158	arg1	analysis					1160:1167	Micro Computed Tomography analysis	1134:1167	Micro Computed Tomography analysis	1134:1167	Micro Computed Tomography analysis suggested that more new tissue was formed in the defects treated with the hydrogels.
30946907	1	53	dep	in	172:173	arg1	vivo					175:178	vivo	175:178	vivo	175:178	The present work describes in vitro and in vivo behaviors of thermosensitive composite hydrogels based on polymers/bioactive glass nanoparticles.
30946907	9	54	contain	possess	1337:1343	arg2	properties					1345:1354	properties	1345:1354	properties which make them suitable candidates for use as temporary injectable matrices for bone regeneration	1345:1453	Taken together, our data suggest that the developed injectable composite hydrogels possess properties which make them suitable candidates for use as temporary injectable matrices for bone regeneration.
30946907	9	54	contain	possess	1337:1343	arg1	hydrogels					1327:1335	the developed injectable composite hydrogels	1292:1335	the developed injectable composite hydrogels	1292:1335	Taken together, our data suggest that the developed injectable composite hydrogels possess properties which make them suitable candidates for use as temporary injectable matrices for bone regeneration.
30946907	2	55	theme	mass	353:356	arg1	loss					336:339	loss	336:339	loss of hydrogel mass in vitro	336:365	Assays in SBF (simulated body fluid) solution showed that loss of hydrogel mass in vitro was decreased by 4.3% when bioactive glass nanoparticles (nBG) were incorporated, and confirmed the bioactivity of nBG containing hydrogels.
30946907	0	56	theme	potential	72:80	arg1	regeneration					87:98	potential bone regeneration	72:98	potential bone regeneration	72:98	Injectable chitosan/gelatin/bioactive glass nanocomposite hydrogels for potential bone regeneration: In vitro and in vivo analyses.
30946907	6	57	theme	subcutaneous	922:933	arg1	injection					935:943	subcutaneous injection	922:943	subcutaneous injection	922:943	The results indicated that the prepared hydrogels were nontoxic upon subcutaneous injection, and could be candidates for a safe in situ gel-forming system.
30946907	7	58	theme	hydrogels	1026:1034	arg1	Injection					1009:1017	Injection	1009:1017	Injection of the hydrogels into a rat tibial defect	1009:1059	Injection of the hydrogels into a rat tibial defect allowed preliminary evaluation of the hydrogels' regenerative potential.
30946907	0	59	dep	hydrogels	58:66	arg1	analyses					122:129	In vitro and in vivo analyses	101:129	Injectable chitosan/gelatin/bioactive glass nanocomposite hydrogels for potential bone regeneration: In vitro and in vivo analyses.	0:130	Injectable chitosan/gelatin/bioactive glass nanocomposite hydrogels for potential bone regeneration: In vitro and in vivo analyses.
30946907	2	60	theme	hydrogel	344:351	arg1	mass					353:356	hydrogel mass	344:356	hydrogel mass	344:356	Assays in SBF (simulated body fluid) solution showed that loss of hydrogel mass in vitro was decreased by 4.3% when bioactive glass nanoparticles (nBG) were incorporated, and confirmed the bioactivity of nBG containing hydrogels.
30946907	4	61	contain	containing	745:754	arg2	nBG					756:758	nBG	756:758	nBG	756:758	Crystal violet assays showed a 27% increase in cell viability when these cells were seeded in hydrogels containing nBG.
30946907	4	61	contain	containing	745:754	arg1	hydrogels					735:743	hydrogels	735:743	hydrogels containing nBG	735:758	Crystal violet assays showed a 27% increase in cell viability when these cells were seeded in hydrogels containing nBG.
30946907	7	62	theme	potential	1123:1131	arg1	evaluation					1081:1090	preliminary evaluation	1069:1090	preliminary evaluation of the hydrogels' regenerative potential	1069:1131	Injection of the hydrogels into a rat tibial defect allowed preliminary evaluation of the hydrogels' regenerative potential.
30946907	2	63	theme	SBF	288:290	arg1	solution					315:322	SBF (simulated body fluid) solution	288:322	SBF (simulated body fluid) solution	288:322	Assays in SBF (simulated body fluid) solution showed that loss of hydrogel mass in vitro was decreased by 4.3% when bioactive glass nanoparticles (nBG) were incorporated, and confirmed the bioactivity of nBG containing hydrogels.
30946907	2	64	contain	containing	486:495	arg1	nBG					482:484	nBG	482:484	nBG containing hydrogels	482:505	Assays in SBF (simulated body fluid) solution showed that loss of hydrogel mass in vitro was decreased by 4.3% when bioactive glass nanoparticles (nBG) were incorporated, and confirmed the bioactivity of nBG containing hydrogels.
30946907	2	64	contain	containing	486:495	arg2	hydrogels					497:505	hydrogels	497:505	hydrogels	497:505	Assays in SBF (simulated body fluid) solution showed that loss of hydrogel mass in vitro was decreased by 4.3% when bioactive glass nanoparticles (nBG) were incorporated, and confirmed the bioactivity of nBG containing hydrogels.
30946907	1	65	theme	present	136:142	arg1	work					144:147	The present work	132:147	The present work	132:147	The present work describes in vitro and in vivo behaviors of thermosensitive composite hydrogels based on polymers/bioactive glass nanoparticles.
30946907	9	66	theme	injectable	1413:1422	arg1	matrices					1424:1431	temporary injectable matrices	1403:1431	temporary injectable matrices for bone regeneration	1403:1453	Taken together, our data suggest that the developed injectable composite hydrogels possess properties which make them suitable candidates for use as temporary injectable matrices for bone regeneration.
30946907	4	67	theme	27	672:673	arg1	%					674:674	%	674:674	%	674:674	Crystal violet assays showed a 27% increase in cell viability when these cells were seeded in hydrogels containing nBG.
30946907	1	68	theme	polymers/bioactive	238:255	arg1	nanoparticles					263:275	polymers/bioactive glass nanoparticles	238:275	polymers/bioactive glass nanoparticles	238:275	The present work describes in vitro and in vivo behaviors of thermosensitive composite hydrogels based on polymers/bioactive glass nanoparticles.
30946907	3	69	theme	mesenchymal	610:620	arg1	BMSC					634:637	BMSC	634:637	BMSC	634:637	In vitro assays demonstrated the cytocompatibility of the hydrogels with encapsulated rat bone marrow mesenchymal stem cells (BMSC).
30946907	3	69	theme	mesenchymal	610:620	arg1	cells					627:631	encapsulated rat bone marrow mesenchymal stem cells	581:631	encapsulated rat bone marrow mesenchymal stem cells (BMSC)	581:638	In vitro assays demonstrated the cytocompatibility of the hydrogels with encapsulated rat bone marrow mesenchymal stem cells (BMSC).
30946907	3	70	theme	In	508:509	arg1	assays					517:522	In vitro assays	508:522	In vitro assays	508:522	In vitro assays demonstrated the cytocompatibility of the hydrogels with encapsulated rat bone marrow mesenchymal stem cells (BMSC).
30946907	5	71	theme	rats	847:850	arg1	dorsum					831:836	the dorsum	827:836	the dorsum of Swiss rats	827:850	In vivo biocompatibility was examined by injecting hydrogels into the dorsum of Swiss rats.
30946907	1	72	theme	glass	257:261	arg1	nanoparticles					263:275	polymers/bioactive glass nanoparticles	238:275	polymers/bioactive glass nanoparticles	238:275	The present work describes in vitro and in vivo behaviors of thermosensitive composite hydrogels based on polymers/bioactive glass nanoparticles.
30946907	2	73	theme	nBG	482:484	arg1	bioactivity					467:477	the bioactivity	463:477	the bioactivity of nBG containing hydrogels	463:505	Assays in SBF (simulated body fluid) solution showed that loss of hydrogel mass in vitro was decreased by 4.3% when bioactive glass nanoparticles (nBG) were incorporated, and confirmed the bioactivity of nBG containing hydrogels.
30946907	3	74	theme	stem	622:625	arg1	BMSC					634:637	BMSC	634:637	BMSC	634:637	In vitro assays demonstrated the cytocompatibility of the hydrogels with encapsulated rat bone marrow mesenchymal stem cells (BMSC).
30946907	3	74	theme	stem	622:625	arg1	cells					627:631	encapsulated rat bone marrow mesenchymal stem cells	581:631	encapsulated rat bone marrow mesenchymal stem cells (BMSC)	581:638	In vitro assays demonstrated the cytocompatibility of the hydrogels with encapsulated rat bone marrow mesenchymal stem cells (BMSC).
30946907	1	75	theme	in	159:160	arg1	behaviors					180:188	in vitro and in vivo behaviors	159:188	in vitro and in vivo behaviors of thermosensitive composite hydrogels based on polymers/bioactive glass nanoparticles	159:275	The present work describes in vitro and in vivo behaviors of thermosensitive composite hydrogels based on polymers/bioactive glass nanoparticles.
30314665	3	0	theme	multi-scale	308:318	arg1	problem					320:326	This multi-scale problem	303:326	This multi-scale problem	303:326	This multi-scale problem is framed by the creation of diffusion models of signal decay based on physical laws, histological structure, and biophysical constraints.
30314665	6	1	theme	functional	826:835	arg1	important					863:871	important	863:871	important	863:871	Since this is a heuristic model, the exact functional form of this decay is not important, so here we examine a simple exponential function, D(b) = D0exp(-bD1), where D0 and D1 capture aspects of hindered and restricted diffusion, respectively.
30314665	6	1	theme	functional	826:835	arg1	so					874:875	so	874:875	so	874:875	Since this is a heuristic model, the exact functional form of this decay is not important, so here we examine a simple exponential function, D(b) = D0exp(-bD1), where D0 and D1 capture aspects of hindered and restricted diffusion, respectively.
30314665	6	1	theme	functional	826:835	arg1	form					837:840	the exact functional form	816:840	the exact functional form of this decay	816:854	Since this is a heuristic model, the exact functional form of this decay is not important, so here we examine a simple exponential function, D(b) = D0exp(-bD1), where D0 and D1 capture aspects of hindered and restricted diffusion, respectively.
30314665	9	2	theme	fiber	1458:1462	arg1	phantoms					1464:1471	nested muscle fiber phantoms	1444:1471	nested muscle fiber phantoms whose permeability, atrophy, and fiber size distribution could be changed	1444:1545	Finally, we applied the VDC model to dMRI simulations of nested muscle fiber phantoms whose permeability, atrophy, and fiber size distribution could be changed.
30314665	7	3	theme	model	1070:1074	arg1	potential					1049:1057	the potential	1045:1057	the potential of the VDC model	1045:1074	As an example of the potential of the VDC model, we applied it to dMRI data collected from normal and diseased human brain tissue using Stejskal-Tanner diffusion gradient pulses.
30314665	10	4	theme	tissue	1619:1624	arg1	structure					1626:1634	sub-voxel tissue structure	1609:1634	sub-voxel tissue structure	1609:1634	These results demonstrate that the VDC model is sensitive to sub-voxel tissue structure and composition (porosity, tortuosity, and permeability), hence can capture tissue complexity in a manner that could be easily applied in clinical dMRI.
30314665	4	5	theme	observed	554:561	arg1	decay					563:567	the observed decay	550:567	the observed decay in the effective diffusion coefficient as a function of diffusion weighting (b-value), D(b), that is often observed in complex biological tissues	550:713	The purpose of this paper is to simplify the model building process by focusing on the observed decay in the effective diffusion coefficient as a function of diffusion weighting (b-value), D(b), that is often observed in complex biological tissues.
30314665	7	6	theme	potential	1049:1057	arg1	example					1034:1040	an example	1031:1040	an example of the potential of the VDC model	1031:1074	As an example of the potential of the VDC model, we applied it to dMRI data collected from normal and diseased human brain tissue using Stejskal-Tanner diffusion gradient pulses.
30314665	7	6	theme	potential	1049:1057	arg1	it					1088:1089	it	1088:1089	it	1088:1089	As an example of the potential of the VDC model, we applied it to dMRI data collected from normal and diseased human brain tissue using Stejskal-Tanner diffusion gradient pulses.
30314665	6	7	theme	exact	820:824	arg1	important					863:871	important	863:871	important	863:871	Since this is a heuristic model, the exact functional form of this decay is not important, so here we examine a simple exponential function, D(b) = D0exp(-bD1), where D0 and D1 capture aspects of hindered and restricted diffusion, respectively.
30314665	6	7	theme	exact	820:824	arg1	so					874:875	so	874:875	so	874:875	Since this is a heuristic model, the exact functional form of this decay is not important, so here we examine a simple exponential function, D(b) = D0exp(-bD1), where D0 and D1 capture aspects of hindered and restricted diffusion, respectively.
30314665	6	7	theme	exact	820:824	arg1	form					837:840	the exact functional form	816:840	the exact functional form of this decay	816:854	Since this is a heuristic model, the exact functional form of this decay is not important, so here we examine a simple exponential function, D(b) = D0exp(-bD1), where D0 and D1 capture aspects of hindered and restricted diffusion, respectively.
30314665	9	8	theme	phantoms	1464:1471	arg1	simulations					1429:1439	dMRI simulations	1424:1439	dMRI simulations of nested muscle fiber phantoms whose permeability, atrophy, and fiber size distribution could be changed	1424:1545	Finally, we applied the VDC model to dMRI simulations of nested muscle fiber phantoms whose permeability, atrophy, and fiber size distribution could be changed.
30314665	8	9	theme	sub-voxel	1271:1279	arg1	structure					1281:1289	the sub-voxel structure	1267:1289	the sub-voxel structure	1267:1289	In order to illustrate the connection between D0 and D1 and the sub-voxel structure we also analyzed dMRI data from families of Sephadex beads selected with increasing tortuosity.
30314665	7	10	theme	brain	1145:1149	arg1	tissue					1151:1156	normal and diseased human brain tissue	1119:1156	normal and diseased human brain tissue	1119:1156	As an example of the potential of the VDC model, we applied it to dMRI data collected from normal and diseased human brain tissue using Stejskal-Tanner diffusion gradient pulses.
30314665	6	11	dep	 = D0exp	928:935	arg1	-bD1					937:940	-bD1	937:940	-bD1	937:940	Since this is a heuristic model, the exact functional form of this decay is not important, so here we examine a simple exponential function, D(b) = D0exp(-bD1), where D0 and D1 capture aspects of hindered and restricted diffusion, respectively.
30314665	4	12	theme	biological	696:705	arg1	tissues					707:713	complex biological tissues	688:713	complex biological tissues	688:713	The purpose of this paper is to simplify the model building process by focusing on the observed decay in the effective diffusion coefficient as a function of diffusion weighting (b-value), D(b), that is often observed in complex biological tissues.
30314665	4	13	theme	weighting	635:643	arg1	function					613:620	a function	611:620	a function of diffusion weighting (b-value)	611:653	The purpose of this paper is to simplify the model building process by focusing on the observed decay in the effective diffusion coefficient as a function of diffusion weighting (b-value), D(b), that is often observed in complex biological tissues.
30314665	4	13	theme	weighting	635:643	arg1	D					656:656	D	656:656	D(b)	656:659	The purpose of this paper is to simplify the model building process by focusing on the observed decay in the effective diffusion coefficient as a function of diffusion weighting (b-value), D(b), that is often observed in complex biological tissues.
30314665	4	14	from	decay	563:567	arg1	coefficient					596:606	the effective diffusion coefficient	572:606	the effective diffusion coefficient as a function of diffusion weighting (b-value), D(b), that is often observed in complex biological tissues	572:713	The purpose of this paper is to simplify the model building process by focusing on the observed decay in the effective diffusion coefficient as a function of diffusion weighting (b-value), D(b), that is often observed in complex biological tissues.
30314665	8	15	theme	beads	1344:1348	arg1	families					1323:1330	families	1323:1330	families of Sephadex beads selected with increasing tortuosity	1323:1384	In order to illustrate the connection between D0 and D1 and the sub-voxel structure we also analyzed dMRI data from families of Sephadex beads selected with increasing tortuosity.
30314665	4	16	theme	complex	688:694	arg1	tissues					707:713	complex biological tissues	688:713	complex biological tissues	688:713	The purpose of this paper is to simplify the model building process by focusing on the observed decay in the effective diffusion coefficient as a function of diffusion weighting (b-value), D(b), that is often observed in complex biological tissues.
30314665	1	17	theme	Diffusion-weighted	64:81	arg1	MRI					83:85	Diffusion-weighted MRI	64:85	Diffusion-weighted MRI (dMRI)	64:92	Diffusion-weighted MRI (dMRI) is a key component of clinical radiology.
30314665	1	17	theme	Diffusion-weighted	64:81	arg1	component					103:111	a key component	97:111	a key component of clinical radiology	97:133	Diffusion-weighted MRI (dMRI) is a key component of clinical radiology.
30314665	1	17	theme	Diffusion-weighted	64:81	arg1	dMRI					88:91	dMRI	88:91	dMRI	88:91	Diffusion-weighted MRI (dMRI) is a key component of clinical radiology.
30314665	6	18	theme	simple	895:900	arg1	function					914:921	a simple exponential function	893:921	a simple exponential function	893:921	Since this is a heuristic model, the exact functional form of this decay is not important, so here we examine a simple exponential function, D(b) = D0exp(-bD1), where D0 and D1 capture aspects of hindered and restricted diffusion, respectively.
30314665	7	19	theme	dMRI	1094:1097	arg1	data					1099:1102	dMRI data	1094:1102	dMRI data collected from normal and diseased human brain tissue using Stejskal-Tanner diffusion gradient pulses	1094:1204	As an example of the potential of the VDC model, we applied it to dMRI data collected from normal and diseased human brain tissue using Stejskal-Tanner diffusion gradient pulses.
30314665	3	20	theme	histological	414:425	arg1	structure					427:435	histological structure	414:435	histological structure	414:435	This multi-scale problem is framed by the creation of diffusion models of signal decay based on physical laws, histological structure, and biophysical constraints.
30314665	6	21	theme	heuristic	799:807	arg1	model					809:813	a heuristic model	797:813	a heuristic model	797:813	Since this is a heuristic model, the exact functional form of this decay is not important, so here we examine a simple exponential function, D(b) = D0exp(-bD1), where D0 and D1 capture aspects of hindered and restricted diffusion, respectively.
30314665	6	21	theme	heuristic	799:807	arg1	this					789:792	this	789:792	this	789:792	Since this is a heuristic model, the exact functional form of this decay is not important, so here we examine a simple exponential function, D(b) = D0exp(-bD1), where D0 and D1 capture aspects of hindered and restricted diffusion, respectively.
30314665	2	22	theme	coefficient	278:288	arg1	measurements					248:259	measurements	248:259	measurements of the diffusion coefficient, D0 (mm2/s)	248:300	When analyzing diffusion-weighted images, radiologists often seek to infer microscopic tissue structure through measurements of the diffusion coefficient, D0 (mm2/s).
30314665	4	23	theme	building	518:525	arg1	process					527:533	the model building process	508:533	the model building process	508:533	The purpose of this paper is to simplify the model building process by focusing on the observed decay in the effective diffusion coefficient as a function of diffusion weighting (b-value), D(b), that is often observed in complex biological tissues.
30314665	10	24	theme	tissue	1712:1717	arg1	complexity					1719:1728	tissue complexity	1712:1728	tissue complexity	1712:1728	These results demonstrate that the VDC model is sensitive to sub-voxel tissue structure and composition (porosity, tortuosity, and permeability), hence can capture tissue complexity in a manner that could be easily applied in clinical dMRI.
30314665	6	25	theme	diffusion	1003:1011	arg1	aspects					968:974	aspects	968:974	aspects of hindered and restricted diffusion	968:1011	Since this is a heuristic model, the exact functional form of this decay is not important, so here we examine a simple exponential function, D(b) = D0exp(-bD1), where D0 and D1 capture aspects of hindered and restricted diffusion, respectively.
30314665	8	26	theme	Sephadex	1335:1342	arg1	beads					1344:1348	Sephadex beads	1335:1348	Sephadex beads	1335:1348	In order to illustrate the connection between D0 and D1 and the sub-voxel structure we also analyzed dMRI data from families of Sephadex beads selected with increasing tortuosity.
30314665	7	27	theme	diseased	1130:1137	arg1	tissue					1151:1156	normal and diseased human brain tissue	1119:1156	normal and diseased human brain tissue	1119:1156	As an example of the potential of the VDC model, we applied it to dMRI data collected from normal and diseased human brain tissue using Stejskal-Tanner diffusion gradient pulses.
30314665	3	28	theme	signal	377:382	arg1	decay					384:388	signal decay	377:388	signal decay	377:388	This multi-scale problem is framed by the creation of diffusion models of signal decay based on physical laws, histological structure, and biophysical constraints.
30314665	10	29	theme	sub-voxel	1609:1617	arg1	structure					1626:1634	sub-voxel tissue structure	1609:1634	sub-voxel tissue structure	1609:1634	These results demonstrate that the VDC model is sensitive to sub-voxel tissue structure and composition (porosity, tortuosity, and permeability), hence can capture tissue complexity in a manner that could be easily applied in clinical dMRI.
30314665	6	30	theme	restricted	992:1001	arg1	diffusion					1003:1011	hindered and restricted diffusion	979:1011	hindered and restricted diffusion	979:1011	Since this is a heuristic model, the exact functional form of this decay is not important, so here we examine a simple exponential function, D(b) = D0exp(-bD1), where D0 and D1 capture aspects of hindered and restricted diffusion, respectively.
30314665	6	31	theme	 = D0exp	928:935	arg1	function					914:921	a simple exponential function	893:921	a simple exponential function	893:921	Since this is a heuristic model, the exact functional form of this decay is not important, so here we examine a simple exponential function, D(b) = D0exp(-bD1), where D0 and D1 capture aspects of hindered and restricted diffusion, respectively.
30314665	3	32	theme	diffusion	357:365	arg1	models					367:372	diffusion models	357:372	diffusion models of signal decay based on physical laws, histological structure, and biophysical constraints	357:464	This multi-scale problem is framed by the creation of diffusion models of signal decay based on physical laws, histological structure, and biophysical constraints.
30314665	5	33	theme	varying	742:748	arg1	model					776:780	the varying diffusion curvature (VDC) model	738:780	the varying diffusion curvature (VDC) model	738:780	We call this approach the varying diffusion curvature (VDC) model.
30314665	9	34	theme	fiber	1506:1510	arg1	distribution					1517:1528	fiber size distribution	1506:1528	fiber size distribution	1506:1528	Finally, we applied the VDC model to dMRI simulations of nested muscle fiber phantoms whose permeability, atrophy, and fiber size distribution could be changed.
30314665	7	35	theme	Stejskal-Tanner	1164:1178	arg1	pulses					1199:1204	Stejskal-Tanner diffusion gradient pulses	1164:1204	Stejskal-Tanner diffusion gradient pulses	1164:1204	As an example of the potential of the VDC model, we applied it to dMRI data collected from normal and diseased human brain tissue using Stejskal-Tanner diffusion gradient pulses.
30314665	3	36	theme	biophysical	442:452	arg1	constraints					454:464	biophysical constraints	442:464	biophysical constraints	442:464	This multi-scale problem is framed by the creation of diffusion models of signal decay based on physical laws, histological structure, and biophysical constraints.
30314665	3	37	theme	models	367:372	arg1	creation					345:352	the creation	341:352	the creation of diffusion models of signal decay based on physical laws, histological structure, and biophysical constraints	341:464	This multi-scale problem is framed by the creation of diffusion models of signal decay based on physical laws, histological structure, and biophysical constraints.
30314665	9	38	theme	size	1512:1515	arg1	distribution					1517:1528	fiber size distribution	1506:1528	fiber size distribution	1506:1528	Finally, we applied the VDC model to dMRI simulations of nested muscle fiber phantoms whose permeability, atrophy, and fiber size distribution could be changed.
30314665	6	39	theme	hindered	979:986	arg1	diffusion					1003:1011	hindered and restricted diffusion	979:1011	hindered and restricted diffusion	979:1011	Since this is a heuristic model, the exact functional form of this decay is not important, so here we examine a simple exponential function, D(b) = D0exp(-bD1), where D0 and D1 capture aspects of hindered and restricted diffusion, respectively.
30314665	5	40	theme	VDC	771:773	arg1	model					776:780	the varying diffusion curvature (VDC) model	738:780	the varying diffusion curvature (VDC) model	738:780	We call this approach the varying diffusion curvature (VDC) model.
30314665	0	41	theme	MR	47:48	arg1	decay					57:61	the diffusion-weighted MR signal decay	24:61	the diffusion-weighted MR signal decay	24:61	Capturing complexity of the diffusion-weighted MR signal decay.
30314665	9	42	theme	VDC	1411:1413	arg1	model					1415:1419	the VDC model	1407:1419	the VDC model	1407:1419	Finally, we applied the VDC model to dMRI simulations of nested muscle fiber phantoms whose permeability, atrophy, and fiber size distribution could be changed.
30314665	7	43	theme	human	1139:1143	arg1	tissue					1151:1156	normal and diseased human brain tissue	1119:1156	normal and diseased human brain tissue	1119:1156	As an example of the potential of the VDC model, we applied it to dMRI data collected from normal and diseased human brain tissue using Stejskal-Tanner diffusion gradient pulses.
30314665	3	44	theme	physical	399:406	arg1	laws					408:411	physical laws	399:411	physical laws	399:411	This multi-scale problem is framed by the creation of diffusion models of signal decay based on physical laws, histological structure, and biophysical constraints.
30314665	0	45	theme	diffusion-weighted	28:45	arg1	decay					57:61	the diffusion-weighted MR signal decay	24:61	the diffusion-weighted MR signal decay	24:61	Capturing complexity of the diffusion-weighted MR signal decay.
30314665	5	46	theme	diffusion	750:758	arg1	model					776:780	the varying diffusion curvature (VDC) model	738:780	the varying diffusion curvature (VDC) model	738:780	We call this approach the varying diffusion curvature (VDC) model.
30314665	10	47	dep	composition	1640:1650	arg1	tortuosity					1663:1672	tortuosity	1663:1672	tortuosity	1663:1672	These results demonstrate that the VDC model is sensitive to sub-voxel tissue structure and composition (porosity, tortuosity, and permeability), hence can capture tissue complexity in a manner that could be easily applied in clinical dMRI.
30314665	10	47	dep	composition	1640:1650	arg1	permeability					1679:1690	permeability	1679:1690	permeability	1679:1690	These results demonstrate that the VDC model is sensitive to sub-voxel tissue structure and composition (porosity, tortuosity, and permeability), hence can capture tissue complexity in a manner that could be easily applied in clinical dMRI.
30314665	10	47	dep	composition	1640:1650	arg1	porosity					1653:1660	porosity	1653:1660	porosity	1653:1660	These results demonstrate that the VDC model is sensitive to sub-voxel tissue structure and composition (porosity, tortuosity, and permeability), hence can capture tissue complexity in a manner that could be easily applied in clinical dMRI.
30314665	3	48	theme	decay	384:388	arg1	models					367:372	diffusion models	357:372	diffusion models of signal decay based on physical laws, histological structure, and biophysical constraints	357:464	This multi-scale problem is framed by the creation of diffusion models of signal decay based on physical laws, histological structure, and biophysical constraints.
30314665	6	49	theme	decay	850:854	arg1	important					863:871	important	863:871	important	863:871	Since this is a heuristic model, the exact functional form of this decay is not important, so here we examine a simple exponential function, D(b) = D0exp(-bD1), where D0 and D1 capture aspects of hindered and restricted diffusion, respectively.
30314665	6	49	theme	decay	850:854	arg1	so					874:875	so	874:875	so	874:875	Since this is a heuristic model, the exact functional form of this decay is not important, so here we examine a simple exponential function, D(b) = D0exp(-bD1), where D0 and D1 capture aspects of hindered and restricted diffusion, respectively.
30314665	6	49	theme	decay	850:854	arg1	form					837:840	the exact functional form	816:840	the exact functional form of this decay	816:854	Since this is a heuristic model, the exact functional form of this decay is not important, so here we examine a simple exponential function, D(b) = D0exp(-bD1), where D0 and D1 capture aspects of hindered and restricted diffusion, respectively.
30314665	2	50	theme	diffusion-weighted	151:168	arg1	images					170:175	diffusion-weighted images	151:175	diffusion-weighted images	151:175	When analyzing diffusion-weighted images, radiologists often seek to infer microscopic tissue structure through measurements of the diffusion coefficient, D0 (mm2/s).
30314665	2	51	theme	tissue	223:228	arg1	structure					230:238	microscopic tissue structure	211:238	microscopic tissue structure	211:238	When analyzing diffusion-weighted images, radiologists often seek to infer microscopic tissue structure through measurements of the diffusion coefficient, D0 (mm2/s).
30314665	8	52	from	families	1323:1330	arg1	data					1313:1316	dMRI data	1308:1316	dMRI data from families of Sephadex beads selected with increasing tortuosity	1308:1384	In order to illustrate the connection between D0 and D1 and the sub-voxel structure we also analyzed dMRI data from families of Sephadex beads selected with increasing tortuosity.
30314665	1	53	theme	key	99:101	arg1	MRI					83:85	Diffusion-weighted MRI	64:85	Diffusion-weighted MRI (dMRI)	64:92	Diffusion-weighted MRI (dMRI) is a key component of clinical radiology.
30314665	1	53	theme	key	99:101	arg1	component					103:111	a key component	97:111	a key component of clinical radiology	97:133	Diffusion-weighted MRI (dMRI) is a key component of clinical radiology.
30314665	0	54	theme	signal	50:55	arg1	decay					57:61	the diffusion-weighted MR signal decay	24:61	the diffusion-weighted MR signal decay	24:61	Capturing complexity of the diffusion-weighted MR signal decay.
30314665	4	55	located	observed	676:683	arg2	D					656:656	D	656:656	D(b)	656:659	The purpose of this paper is to simplify the model building process by focusing on the observed decay in the effective diffusion coefficient as a function of diffusion weighting (b-value), D(b), that is often observed in complex biological tissues.
30314665	4	55	located	observed	676:683	arg1	tissues					707:713	complex biological tissues	688:713	complex biological tissues	688:713	The purpose of this paper is to simplify the model building process by focusing on the observed decay in the effective diffusion coefficient as a function of diffusion weighting (b-value), D(b), that is often observed in complex biological tissues.
30314665	4	55	located	observed	676:683	arg2	function					613:620	a function	611:620	a function of diffusion weighting (b-value)	611:653	The purpose of this paper is to simplify the model building process by focusing on the observed decay in the effective diffusion coefficient as a function of diffusion weighting (b-value), D(b), that is often observed in complex biological tissues.
30314665	6	56	dep	important	863:871	arg1	important					863:871	important	863:871	important	863:871	Since this is a heuristic model, the exact functional form of this decay is not important, so here we examine a simple exponential function, D(b) = D0exp(-bD1), where D0 and D1 capture aspects of hindered and restricted diffusion, respectively.
30314665	6	56	dep	important	863:871	arg1	form					837:840	the exact functional form	816:840	the exact functional form of this decay	816:854	Since this is a heuristic model, the exact functional form of this decay is not important, so here we examine a simple exponential function, D(b) = D0exp(-bD1), where D0 and D1 capture aspects of hindered and restricted diffusion, respectively.
30314665	6	56	dep	important	863:871	arg1	so					874:875	so	874:875	so	874:875	Since this is a heuristic model, the exact functional form of this decay is not important, so here we examine a simple exponential function, D(b) = D0exp(-bD1), where D0 and D1 capture aspects of hindered and restricted diffusion, respectively.
30314665	2	57	theme	microscopic	211:221	arg1	structure					230:238	microscopic tissue structure	211:238	microscopic tissue structure	211:238	When analyzing diffusion-weighted images, radiologists often seek to infer microscopic tissue structure through measurements of the diffusion coefficient, D0 (mm2/s).
30314665	10	58	theme	VDC	1583:1585	arg1	model					1587:1591	the VDC model	1579:1591	the VDC model	1579:1591	These results demonstrate that the VDC model is sensitive to sub-voxel tissue structure and composition (porosity, tortuosity, and permeability), hence can capture tissue complexity in a manner that could be easily applied in clinical dMRI.
30314665	10	58	theme	VDC	1583:1585	arg1	sensitive					1596:1604	sensitive	1596:1604	sensitive	1596:1604	These results demonstrate that the VDC model is sensitive to sub-voxel tissue structure and composition (porosity, tortuosity, and permeability), hence can capture tissue complexity in a manner that could be easily applied in clinical dMRI.
30314665	4	59	theme	model	512:516	arg1	building					518:525	the model building	508:525	the model building process	508:533	The purpose of this paper is to simplify the model building process by focusing on the observed decay in the effective diffusion coefficient as a function of diffusion weighting (b-value), D(b), that is often observed in complex biological tissues.
30314665	7	60	theme	diffusion	1180:1188	arg1	pulses					1199:1204	Stejskal-Tanner diffusion gradient pulses	1164:1204	Stejskal-Tanner diffusion gradient pulses	1164:1204	As an example of the potential of the VDC model, we applied it to dMRI data collected from normal and diseased human brain tissue using Stejskal-Tanner diffusion gradient pulses.
30314665	2	61	theme	diffusion	268:276	arg1	D0					291:292	D0	291:292	D0 (mm2/s)	291:300	When analyzing diffusion-weighted images, radiologists often seek to infer microscopic tissue structure through measurements of the diffusion coefficient, D0 (mm2/s).
30314665	2	61	theme	diffusion	268:276	arg1	coefficient					278:288	the diffusion coefficient	264:288	the diffusion coefficient	264:288	When analyzing diffusion-weighted images, radiologists often seek to infer microscopic tissue structure through measurements of the diffusion coefficient, D0 (mm2/s).
30314665	9	62	theme	muscle	1451:1456	arg1	phantoms					1464:1471	nested muscle fiber phantoms	1444:1471	nested muscle fiber phantoms whose permeability, atrophy, and fiber size distribution could be changed	1444:1545	Finally, we applied the VDC model to dMRI simulations of nested muscle fiber phantoms whose permeability, atrophy, and fiber size distribution could be changed.
30314665	4	63	theme	effective	576:584	arg1	coefficient					596:606	the effective diffusion coefficient	572:606	the effective diffusion coefficient as a function of diffusion weighting (b-value), D(b), that is often observed in complex biological tissues	572:713	The purpose of this paper is to simplify the model building process by focusing on the observed decay in the effective diffusion coefficient as a function of diffusion weighting (b-value), D(b), that is often observed in complex biological tissues.
30314665	8	64	theme	dMRI	1308:1311	arg1	data					1313:1316	dMRI data	1308:1316	dMRI data from families of Sephadex beads selected with increasing tortuosity	1308:1384	In order to illustrate the connection between D0 and D1 and the sub-voxel structure we also analyzed dMRI data from families of Sephadex beads selected with increasing tortuosity.
30314665	7	65	theme	gradient	1190:1197	arg1	pulses					1199:1204	Stejskal-Tanner diffusion gradient pulses	1164:1204	Stejskal-Tanner diffusion gradient pulses	1164:1204	As an example of the potential of the VDC model, we applied it to dMRI data collected from normal and diseased human brain tissue using Stejskal-Tanner diffusion gradient pulses.
30314665	1	66	theme	clinical	116:123	arg1	radiology					125:133	clinical radiology	116:133	clinical radiology	116:133	Diffusion-weighted MRI (dMRI) is a key component of clinical radiology.
30314665	7	67	theme	VDC	1066:1068	arg1	model					1070:1074	the VDC model	1062:1074	the VDC model	1062:1074	As an example of the potential of the VDC model, we applied it to dMRI data collected from normal and diseased human brain tissue using Stejskal-Tanner diffusion gradient pulses.
30314665	9	68	theme	nested	1444:1449	arg1	phantoms					1464:1471	nested muscle fiber phantoms	1444:1471	nested muscle fiber phantoms whose permeability, atrophy, and fiber size distribution could be changed	1444:1545	Finally, we applied the VDC model to dMRI simulations of nested muscle fiber phantoms whose permeability, atrophy, and fiber size distribution could be changed.
30314665	7	69	theme	normal	1119:1124	arg1	tissue					1151:1156	normal and diseased human brain tissue	1119:1156	normal and diseased human brain tissue	1119:1156	As an example of the potential of the VDC model, we applied it to dMRI data collected from normal and diseased human brain tissue using Stejskal-Tanner diffusion gradient pulses.
30314665	1	70	theme	radiology	125:133	arg1	MRI					83:85	Diffusion-weighted MRI	64:85	Diffusion-weighted MRI (dMRI)	64:92	Diffusion-weighted MRI (dMRI) is a key component of clinical radiology.
30314665	1	70	theme	radiology	125:133	arg1	component					103:111	a key component	97:111	a key component of clinical radiology	97:133	Diffusion-weighted MRI (dMRI) is a key component of clinical radiology.
30314665	4	71	theme	diffusion	625:633	arg1	weighting					635:643	diffusion weighting	625:643	diffusion weighting (b-value)	625:653	The purpose of this paper is to simplify the model building process by focusing on the observed decay in the effective diffusion coefficient as a function of diffusion weighting (b-value), D(b), that is often observed in complex biological tissues.
30314665	4	71	theme	diffusion	625:633	arg1	b-value					646:652	b-value	646:652	b-value	646:652	The purpose of this paper is to simplify the model building process by focusing on the observed decay in the effective diffusion coefficient as a function of diffusion weighting (b-value), D(b), that is often observed in complex biological tissues.
30314665	6	72	theme	exponential	902:912	arg1	function					914:921	a simple exponential function	893:921	a simple exponential function	893:921	Since this is a heuristic model, the exact functional form of this decay is not important, so here we examine a simple exponential function, D(b) = D0exp(-bD1), where D0 and D1 capture aspects of hindered and restricted diffusion, respectively.
30314665	0	73	theme	decay	57:61	arg1	complexity					10:19	complexity	10:19	complexity of the diffusion-weighted MR signal decay	10:61	Capturing complexity of the diffusion-weighted MR signal decay.
30314665	4	74	theme	paper	487:491	arg1	purpose					471:477	The purpose	467:477	The purpose of this paper	467:491	The purpose of this paper is to simplify the model building process by focusing on the observed decay in the effective diffusion coefficient as a function of diffusion weighting (b-value), D(b), that is often observed in complex biological tissues.
30314665	5	75	theme	curvature	760:768	arg1	model					776:780	the varying diffusion curvature (VDC) model	738:780	the varying diffusion curvature (VDC) model	738:780	We call this approach the varying diffusion curvature (VDC) model.
30314665	10	76	theme	clinical	1774:1781	arg1	dMRI					1783:1786	clinical dMRI	1774:1786	clinical dMRI	1774:1786	These results demonstrate that the VDC model is sensitive to sub-voxel tissue structure and composition (porosity, tortuosity, and permeability), hence can capture tissue complexity in a manner that could be easily applied in clinical dMRI.
30314665	9	77	theme	dMRI	1424:1427	arg1	simulations					1429:1439	dMRI simulations	1424:1439	dMRI simulations of nested muscle fiber phantoms whose permeability, atrophy, and fiber size distribution could be changed	1424:1545	Finally, we applied the VDC model to dMRI simulations of nested muscle fiber phantoms whose permeability, atrophy, and fiber size distribution could be changed.
30314665	4	78	theme	diffusion	586:594	arg1	coefficient					596:606	the effective diffusion coefficient	572:606	the effective diffusion coefficient as a function of diffusion weighting (b-value), D(b), that is often observed in complex biological tissues	572:713	The purpose of this paper is to simplify the model building process by focusing on the observed decay in the effective diffusion coefficient as a function of diffusion weighting (b-value), D(b), that is often observed in complex biological tissues.
31640819	2	0	theme	intestinal	633:642	arg1	dysfunction					644:654	weaning associated intestinal dysfunction	614:654	weaning associated intestinal dysfunction	614:654	The objective of this experiment was to determine the effects of increasing dietary inclusion levels of laminarin in the first 14 d post-weaning on pig growth performance and weaning associated intestinal dysfunction.
31640819	3	1	theme	ppm	874:876	arg1	laminarin					879:887	100 parts per million (ppm) laminarin	851:887	100 parts per million (ppm) laminarin	851:887	At weaning, ninety-six pigs (8·4 (sd 1·09) kg) (meatline boars × (large white × landrace sows)) were blocked by live weight, litter and sex and randomly assigned to: (1) basal diet; (2) basal + 100 parts per million (ppm) laminarin; (3) basal + 200 ppm laminarin and (4) basal + 300 ppm laminarin (three pigs/pen).
31640819	9	2	theme	molar	1876:1880	arg1	proportions					1882:1892	the molar proportions	1872:1892	the molar proportions of butyrate	1872:1904	Laminarin supplementation reduced Enterobacteriaceae numbers in the caecum (P < 0·05) and increased lactobacilli numbers (P < 0·05), total volatile fatty acid concentrations and the molar proportions of butyrate (P < 0·01) in the colon.
31640819	2	3	theme	weaning	614:620	arg1	dysfunction					644:654	weaning associated intestinal dysfunction	614:654	weaning associated intestinal dysfunction	614:654	The objective of this experiment was to determine the effects of increasing dietary inclusion levels of laminarin in the first 14 d post-weaning on pig growth performance and weaning associated intestinal dysfunction.
31640819	4	4	theme	laminarin	1119:1127	arg1	levels					1109:1114	the above dietary inclusion levels	1081:1114	the above dietary inclusion levels of laminarin	1081:1127	The appropriate quantity of a laminarin-rich extract (65 % laminarin) was added to the basal diet to achieve the above dietary inclusion levels of laminarin.
31640819	1	5	from	health	348:353	arg1	absence					362:368	the absence	358:368	the absence of in-feed medications	358:391	The identification of natural bioactive compounds which can prevent the post-weaning growth check and enhance gastrointestinal health in the absence of in-feed medications is an urgent priority for the swine industry.
31640819	1	6	theme	post-weaning	293:304	arg1	check					313:317	the post-weaning growth check	289:317	the post-weaning growth check	289:317	The identification of natural bioactive compounds which can prevent the post-weaning growth check and enhance gastrointestinal health in the absence of in-feed medications is an urgent priority for the swine industry.
31640819	3	7	theme	meatline	705:712	arg1	boars					714:718	meatline boars ×	705:720	meatline boars × (large white × landrace sows)	705:750	At weaning, ninety-six pigs (8·4 (sd 1·09) kg) (meatline boars × (large white × landrace sows)) were blocked by live weight, litter and sex and randomly assigned to: (1) basal diet; (2) basal + 100 parts per million (ppm) laminarin; (3) basal + 200 ppm laminarin and (4) basal + 300 ppm laminarin (three pigs/pen).
31640819	3	7	theme	meatline	705:712	arg1	sows					746:749	large white × landrace sows	723:749	large white × landrace sows	723:749	At weaning, ninety-six pigs (8·4 (sd 1·09) kg) (meatline boars × (large white × landrace sows)) were blocked by live weight, litter and sex and randomly assigned to: (1) basal diet; (2) basal + 100 parts per million (ppm) laminarin; (3) basal + 200 ppm laminarin and (4) basal + 300 ppm laminarin (three pigs/pen).
31640819	6	8	theme	higher	1331:1336	arg1	ADFI					1338:1341	higher ADFI	1331:1341	higher ADFI	1331:1341	The 300 ppm laminarin group was selected as this group had higher ADFI compared with all other groups and higher ADG than the basal group (P < 0·05).
31640819	3	9	theme	ppm	906:908	arg1	laminarin					910:918	200 ppm laminarin	902:918	200 ppm laminarin	902:918	At weaning, ninety-six pigs (8·4 (sd 1·09) kg) (meatline boars × (large white × landrace sows)) were blocked by live weight, litter and sex and randomly assigned to: (1) basal diet; (2) basal + 100 parts per million (ppm) laminarin; (3) basal + 200 ppm laminarin and (4) basal + 300 ppm laminarin (three pigs/pen).
31640819	3	9	theme	ppm	906:908	arg1	pigs/pen					961:968	three pigs/pen	955:968	three pigs/pen	955:968	At weaning, ninety-six pigs (8·4 (sd 1·09) kg) (meatline boars × (large white × landrace sows)) were blocked by live weight, litter and sex and randomly assigned to: (1) basal diet; (2) basal + 100 parts per million (ppm) laminarin; (3) basal + 200 ppm laminarin and (4) basal + 300 ppm laminarin (three pigs/pen).
31640819	10	10	theme	post-weaning	2069:2080	arg1	dysfunction					2093:2103	post-weaning intestinal dysfunction	2069:2103	post-weaning intestinal dysfunction	2069:2103	In conclusion, 300 ppm laminarin from a laminarin-rich extract has potential, as a dietary supplement, to improve performance and prevent post-weaning intestinal dysfunction.
31640819	3	11	theme	×	735:735	arg1	boars					714:718	meatline boars ×	705:720	meatline boars × (large white × landrace sows)	705:750	At weaning, ninety-six pigs (8·4 (sd 1·09) kg) (meatline boars × (large white × landrace sows)) were blocked by live weight, litter and sex and randomly assigned to: (1) basal diet; (2) basal + 100 parts per million (ppm) laminarin; (3) basal + 200 ppm laminarin and (4) basal + 300 ppm laminarin (three pigs/pen).
31640819	3	11	theme	×	735:735	arg1	sows					746:749	large white × landrace sows	723:749	large white × landrace sows	723:749	At weaning, ninety-six pigs (8·4 (sd 1·09) kg) (meatline boars × (large white × landrace sows)) were blocked by live weight, litter and sex and randomly assigned to: (1) basal diet; (2) basal + 100 parts per million (ppm) laminarin; (3) basal + 200 ppm laminarin and (4) basal + 300 ppm laminarin (three pigs/pen).
31640819	5	12	theme	laminarin	1217:1225	arg1	group					1227:1231	the best-performing laminarin group	1197:1231	the best-performing laminarin group	1197:1231	After 14 d of supplementation, eight pigs from the basal group and the best-performing laminarin group were euthanised for sample collection.
31640819	3	13	theme	large	723:727	arg1	boars					714:718	meatline boars ×	705:720	meatline boars × (large white × landrace sows)	705:750	At weaning, ninety-six pigs (8·4 (sd 1·09) kg) (meatline boars × (large white × landrace sows)) were blocked by live weight, litter and sex and randomly assigned to: (1) basal diet; (2) basal + 100 parts per million (ppm) laminarin; (3) basal + 200 ppm laminarin and (4) basal + 300 ppm laminarin (three pigs/pen).
31640819	3	13	theme	large	723:727	arg1	sows					746:749	large white × landrace sows	723:749	large white × landrace sows	723:749	At weaning, ninety-six pigs (8·4 (sd 1·09) kg) (meatline boars × (large white × landrace sows)) were blocked by live weight, litter and sex and randomly assigned to: (1) basal diet; (2) basal + 100 parts per million (ppm) laminarin; (3) basal + 200 ppm laminarin and (4) basal + 300 ppm laminarin (three pigs/pen).
31640819	3	14	dep	blocked	758:764	arg1	laminarin					910:918	200 ppm laminarin	902:918	200 ppm laminarin	902:918	At weaning, ninety-six pigs (8·4 (sd 1·09) kg) (meatline boars × (large white × landrace sows)) were blocked by live weight, litter and sex and randomly assigned to: (1) basal diet; (2) basal + 100 parts per million (ppm) laminarin; (3) basal + 200 ppm laminarin and (4) basal + 300 ppm laminarin (three pigs/pen).
31640819	3	14	dep	blocked	758:764	arg1	diet					833:836	(1) basal diet	823:836	(1) basal diet	823:836	At weaning, ninety-six pigs (8·4 (sd 1·09) kg) (meatline boars × (large white × landrace sows)) were blocked by live weight, litter and sex and randomly assigned to: (1) basal diet; (2) basal + 100 parts per million (ppm) laminarin; (3) basal + 200 ppm laminarin and (4) basal + 300 ppm laminarin (three pigs/pen).
31640819	3	14	dep	blocked	758:764	arg1	pigs/pen					961:968	three pigs/pen	955:968	three pigs/pen	955:968	At weaning, ninety-six pigs (8·4 (sd 1·09) kg) (meatline boars × (large white × landrace sows)) were blocked by live weight, litter and sex and randomly assigned to: (1) basal diet; (2) basal + 100 parts per million (ppm) laminarin; (3) basal + 200 ppm laminarin and (4) basal + 300 ppm laminarin (three pigs/pen).
31640819	3	14	dep	blocked	758:764	arg1	laminarin					879:887	100 parts per million (ppm) laminarin	851:887	100 parts per million (ppm) laminarin	851:887	At weaning, ninety-six pigs (8·4 (sd 1·09) kg) (meatline boars × (large white × landrace sows)) were blocked by live weight, litter and sex and randomly assigned to: (1) basal diet; (2) basal + 100 parts per million (ppm) laminarin; (3) basal + 200 ppm laminarin and (4) basal + 300 ppm laminarin (three pigs/pen).
31640819	9	15	theme	Enterobacteriaceae	1728:1745	arg1	numbers					1747:1753	Enterobacteriaceae numbers	1728:1753	Enterobacteriaceae numbers in the caecum (P < 0·05)	1728:1778	Laminarin supplementation reduced Enterobacteriaceae numbers in the caecum (P < 0·05) and increased lactobacilli numbers (P < 0·05), total volatile fatty acid concentrations and the molar proportions of butyrate (P < 0·01) in the colon.
31640819	8	16	from	expression	1552:1561	arg1	FABP2					1642:1646	FABP2	1642:1646	FABP2	1642:1646	Laminarin supplementation increased the expression of SLC2A8/GLUT8 in the duodenum, SLC2A2/GLUT2, SLC2A7/GLUT7, SLC15A1/PEPT1 and FABP2 in the jejunum and SLC16A1/MCT1 in the colon.
31640819	8	16	from	expression	1552:1561	arg1	duodenum					1586:1593	duodenum	1586:1593	duodenum	1586:1593	Laminarin supplementation increased the expression of SLC2A8/GLUT8 in the duodenum, SLC2A2/GLUT2, SLC2A7/GLUT7, SLC15A1/PEPT1 and FABP2 in the jejunum and SLC16A1/MCT1 in the colon.
31640819	8	16	from	expression	1552:1561	arg1	SLC15A1/PEPT1					1624:1636	SLC15A1/PEPT1	1624:1636	SLC15A1/PEPT1	1624:1636	Laminarin supplementation increased the expression of SLC2A8/GLUT8 in the duodenum, SLC2A2/GLUT2, SLC2A7/GLUT7, SLC15A1/PEPT1 and FABP2 in the jejunum and SLC16A1/MCT1 in the colon.
31640819	8	16	from	expression	1552:1561	arg1	SLC2A7/GLUT7					1610:1621	SLC2A7/GLUT7	1610:1621	SLC2A7/GLUT7	1610:1621	Laminarin supplementation increased the expression of SLC2A8/GLUT8 in the duodenum, SLC2A2/GLUT2, SLC2A7/GLUT7, SLC15A1/PEPT1 and FABP2 in the jejunum and SLC16A1/MCT1 in the colon.
31640819	8	16	from	expression	1552:1561	arg1	SLC2A2/GLUT2					1596:1607	SLC2A2/GLUT2	1596:1607	SLC2A2/GLUT2	1596:1607	Laminarin supplementation increased the expression of SLC2A8/GLUT8 in the duodenum, SLC2A2/GLUT2, SLC2A7/GLUT7, SLC15A1/PEPT1 and FABP2 in the jejunum and SLC16A1/MCT1 in the colon.
31640819	0	17	theme	intestinal	136:145	arg1	composition					157:167	the large intestinal microbial composition	126:167	the large intestinal microbial composition of piglets	126:178	Laminarin-rich extract improves growth performance, small intestinal morphology, gene expression of nutrient transporters and the large intestinal microbial composition of piglets during the critical post-weaning period.
31640819	1	18	theme	swine	423:427	arg1	industry					429:436	the swine industry	419:436	the swine industry	419:436	The identification of natural bioactive compounds which can prevent the post-weaning growth check and enhance gastrointestinal health in the absence of in-feed medications is an urgent priority for the swine industry.
31640819	8	19	theme	Laminarin	1512:1520	arg1	supplementation					1522:1536	Laminarin supplementation	1512:1536	Laminarin supplementation	1512:1536	Laminarin supplementation increased the expression of SLC2A8/GLUT8 in the duodenum, SLC2A2/GLUT2, SLC2A7/GLUT7, SLC15A1/PEPT1 and FABP2 in the jejunum and SLC16A1/MCT1 in the colon.
31640819	10	20	theme	intestinal	2082:2091	arg1	dysfunction					2093:2103	post-weaning intestinal dysfunction	2069:2103	post-weaning intestinal dysfunction	2069:2103	In conclusion, 300 ppm laminarin from a laminarin-rich extract has potential, as a dietary supplement, to improve performance and prevent post-weaning intestinal dysfunction.
31640819	4	21	theme	laminarin-rich	1002:1015	arg1	extract					1017:1023	a laminarin-rich extract	1000:1023	a laminarin-rich extract (65 % laminarin)	1000:1040	The appropriate quantity of a laminarin-rich extract (65 % laminarin) was added to the basal diet to achieve the above dietary inclusion levels of laminarin.
31640819	4	21	theme	laminarin-rich	1002:1015	arg1	laminarin					1031:1039	65 % laminarin	1026:1039	65 % laminarin	1026:1039	The appropriate quantity of a laminarin-rich extract (65 % laminarin) was added to the basal diet to achieve the above dietary inclusion levels of laminarin.
31640819	6	22	theme	higher	1378:1383	arg1	ADG					1385:1387	higher ADG	1378:1387	higher ADG	1378:1387	The 300 ppm laminarin group was selected as this group had higher ADFI compared with all other groups and higher ADG than the basal group (P < 0·05).
31640819	2	23	theme	pig	587:589	arg1	performance					598:608	pig growth performance	587:608	pig growth performance	587:608	The objective of this experiment was to determine the effects of increasing dietary inclusion levels of laminarin in the first 14 d post-weaning on pig growth performance and weaning associated intestinal dysfunction.
31640819	3	24	theme	live	769:772	arg1	weight					774:779	live weight	769:779	live weight	769:779	At weaning, ninety-six pigs (8·4 (sd 1·09) kg) (meatline boars × (large white × landrace sows)) were blocked by live weight, litter and sex and randomly assigned to: (1) basal diet; (2) basal + 100 parts per million (ppm) laminarin; (3) basal + 200 ppm laminarin and (4) basal + 300 ppm laminarin (three pigs/pen).
31640819	0	25	theme	piglets	172:178	arg1	morphology					69:78	small intestinal morphology	52:78	small intestinal morphology	52:78	Laminarin-rich extract improves growth performance, small intestinal morphology, gene expression of nutrient transporters and the large intestinal microbial composition of piglets during the critical post-weaning period.
31640819	0	25	theme	piglets	172:178	arg1	expression					86:95	gene expression	81:95	gene expression of nutrient transporters	81:120	Laminarin-rich extract improves growth performance, small intestinal morphology, gene expression of nutrient transporters and the large intestinal microbial composition of piglets during the critical post-weaning period.
31640819	0	25	theme	piglets	172:178	arg1	composition					157:167	the large intestinal microbial composition	126:167	the large intestinal microbial composition of piglets	126:178	Laminarin-rich extract improves growth performance, small intestinal morphology, gene expression of nutrient transporters and the large intestinal microbial composition of piglets during the critical post-weaning period.
31640819	0	25	theme	piglets	172:178	arg1	performance					39:49	growth performance	32:49	growth performance	32:49	Laminarin-rich extract improves growth performance, small intestinal morphology, gene expression of nutrient transporters and the large intestinal microbial composition of piglets during the critical post-weaning period.
31640819	7	26	theme	P	1501:1501	arg1	duodenum					1479:1486	duodenum	1479:1486	duodenum	1479:1486	Laminarin supplementation increased villus height in the duodenum and jejunum (P < 0·05).
31640819	7	26	theme	P	1501:1501	arg1	0·05					1505:1508	P < 0·05	1501:1508	P < 0·05	1501:1508	Laminarin supplementation increased villus height in the duodenum and jejunum (P < 0·05).
31640819	4	27	theme	appropriate	976:986	arg1	quantity					988:995	The appropriate quantity	972:995	The appropriate quantity of a laminarin-rich extract (65 % laminarin)	972:1040	The appropriate quantity of a laminarin-rich extract (65 % laminarin) was added to the basal diet to achieve the above dietary inclusion levels of laminarin.
31640819	9	28	from	concentrations	1853:1866	arg1	caecum					1762:1767	the caecum	1758:1767	the caecum (P < 0·05)	1758:1778	Laminarin supplementation reduced Enterobacteriaceae numbers in the caecum (P < 0·05) and increased lactobacilli numbers (P < 0·05), total volatile fatty acid concentrations and the molar proportions of butyrate (P < 0·01) in the colon.
31640819	9	28	from	concentrations	1853:1866	arg1	0·05					1774:1777	P < 0·05	1770:1777	P < 0·05	1770:1777	Laminarin supplementation reduced Enterobacteriaceae numbers in the caecum (P < 0·05) and increased lactobacilli numbers (P < 0·05), total volatile fatty acid concentrations and the molar proportions of butyrate (P < 0·01) in the colon.
31640819	8	29	theme	SLC2A8/GLUT8	1566:1577	arg1	expression					1552:1561	the expression	1548:1561	the expression of SLC2A8/GLUT8 in the duodenum, SLC2A2/GLUT2, SLC2A7/GLUT7, SLC15A1/PEPT1 and FABP2	1548:1646	Laminarin supplementation increased the expression of SLC2A8/GLUT8 in the duodenum, SLC2A2/GLUT2, SLC2A7/GLUT7, SLC15A1/PEPT1 and FABP2 in the jejunum and SLC16A1/MCT1 in the colon.
31640819	2	30	theme	dietary	515:521	arg1	levels					533:538	dietary inclusion levels	515:538	dietary inclusion levels of laminarin	515:551	The objective of this experiment was to determine the effects of increasing dietary inclusion levels of laminarin in the first 14 d post-weaning on pig growth performance and weaning associated intestinal dysfunction.
31640819	0	31	theme	gene	81:84	arg1	expression					86:95	gene expression	81:95	gene expression of nutrient transporters	81:120	Laminarin-rich extract improves growth performance, small intestinal morphology, gene expression of nutrient transporters and the large intestinal microbial composition of piglets during the critical post-weaning period.
31640819	1	32	theme	in-feed	373:379	arg1	medications					381:391	in-feed medications	373:391	in-feed medications	373:391	The identification of natural bioactive compounds which can prevent the post-weaning growth check and enhance gastrointestinal health in the absence of in-feed medications is an urgent priority for the swine industry.
31640819	0	33	theme	post-weaning	200:211	arg1	period					213:218	the critical post-weaning period	187:218	the critical post-weaning period	187:218	Laminarin-rich extract improves growth performance, small intestinal morphology, gene expression of nutrient transporters and the large intestinal microbial composition of piglets during the critical post-weaning period.
31640819	10	34	theme	300	1946:1948	arg1	ppm					1950:1952	ppm	1950:1952	ppm	1950:1952	In conclusion, 300 ppm laminarin from a laminarin-rich extract has potential, as a dietary supplement, to improve performance and prevent post-weaning intestinal dysfunction.
31640819	6	35	theme	<	1413:1413	arg1	group					1404:1408	the basal group	1394:1408	the basal group (P < 0·05)	1394:1419	The 300 ppm laminarin group was selected as this group had higher ADFI compared with all other groups and higher ADG than the basal group (P < 0·05).
31640819	6	35	theme	<	1413:1413	arg1	0·05					1415:1418	P < 0·05	1411:1418	P < 0·05	1411:1418	The 300 ppm laminarin group was selected as this group had higher ADFI compared with all other groups and higher ADG than the basal group (P < 0·05).
31640819	2	36	theme	laminarin	543:551	arg1	levels					533:538	dietary inclusion levels	515:538	dietary inclusion levels of laminarin	515:551	The objective of this experiment was to determine the effects of increasing dietary inclusion levels of laminarin in the first 14 d post-weaning on pig growth performance and weaning associated intestinal dysfunction.
31640819	9	37	theme	lactobacilli	1794:1805	arg1	0·05					1820:1823	P < 0·05	1816:1823	P < 0·05	1816:1823	Laminarin supplementation reduced Enterobacteriaceae numbers in the caecum (P < 0·05) and increased lactobacilli numbers (P < 0·05), total volatile fatty acid concentrations and the molar proportions of butyrate (P < 0·01) in the colon.
31640819	9	37	theme	lactobacilli	1794:1805	arg1	0·01					1911:1914	P < 0·01	1907:1914	P < 0·01	1907:1914	Laminarin supplementation reduced Enterobacteriaceae numbers in the caecum (P < 0·05) and increased lactobacilli numbers (P < 0·05), total volatile fatty acid concentrations and the molar proportions of butyrate (P < 0·01) in the colon.
31640819	9	37	theme	lactobacilli	1794:1805	arg1	numbers					1807:1813	increased lactobacilli numbers	1784:1813	increased lactobacilli numbers (P < 0·05)	1784:1824	Laminarin supplementation reduced Enterobacteriaceae numbers in the caecum (P < 0·05) and increased lactobacilli numbers (P < 0·05), total volatile fatty acid concentrations and the molar proportions of butyrate (P < 0·01) in the colon.
31640819	0	38	theme	transporters	109:120	arg1	morphology					69:78	small intestinal morphology	52:78	small intestinal morphology	52:78	Laminarin-rich extract improves growth performance, small intestinal morphology, gene expression of nutrient transporters and the large intestinal microbial composition of piglets during the critical post-weaning period.
31640819	0	38	theme	transporters	109:120	arg1	expression					86:95	gene expression	81:95	gene expression of nutrient transporters	81:120	Laminarin-rich extract improves growth performance, small intestinal morphology, gene expression of nutrient transporters and the large intestinal microbial composition of piglets during the critical post-weaning period.
31640819	0	38	theme	transporters	109:120	arg1	composition					157:167	the large intestinal microbial composition	126:167	the large intestinal microbial composition of piglets	126:178	Laminarin-rich extract improves growth performance, small intestinal morphology, gene expression of nutrient transporters and the large intestinal microbial composition of piglets during the critical post-weaning period.
31640819	0	38	theme	transporters	109:120	arg1	performance					39:49	growth performance	32:49	growth performance	32:49	Laminarin-rich extract improves growth performance, small intestinal morphology, gene expression of nutrient transporters and the large intestinal microbial composition of piglets during the critical post-weaning period.
31640819	1	39	theme	urgent	399:404	arg1	identification					225:238	The identification	221:238	The identification of natural bioactive compounds which can prevent the post-weaning growth check and enhance gastrointestinal health in the absence of in-feed medications	221:391	The identification of natural bioactive compounds which can prevent the post-weaning growth check and enhance gastrointestinal health in the absence of in-feed medications is an urgent priority for the swine industry.
31640819	1	39	theme	urgent	399:404	arg1	priority					406:413	an urgent priority	396:413	an urgent priority for the swine industry	396:436	The identification of natural bioactive compounds which can prevent the post-weaning growth check and enhance gastrointestinal health in the absence of in-feed medications is an urgent priority for the swine industry.
31640819	3	40	theme	300	936:938	arg1	ppm					940:942	ppm	940:942	ppm	940:942	At weaning, ninety-six pigs (8·4 (sd 1·09) kg) (meatline boars × (large white × landrace sows)) were blocked by live weight, litter and sex and randomly assigned to: (1) basal diet; (2) basal + 100 parts per million (ppm) laminarin; (3) basal + 200 ppm laminarin and (4) basal + 300 ppm laminarin (three pigs/pen).
31640819	3	41	theme	basal	827:831	arg1	diet					833:836	(1) basal diet	823:836	(1) basal diet	823:836	At weaning, ninety-six pigs (8·4 (sd 1·09) kg) (meatline boars × (large white × landrace sows)) were blocked by live weight, litter and sex and randomly assigned to: (1) basal diet; (2) basal + 100 parts per million (ppm) laminarin; (3) basal + 200 ppm laminarin and (4) basal + 300 ppm laminarin (three pigs/pen).
31640819	2	42	from	effects	493:499	arg1	dysfunction					644:654	weaning associated intestinal dysfunction	614:654	weaning associated intestinal dysfunction	614:654	The objective of this experiment was to determine the effects of increasing dietary inclusion levels of laminarin in the first 14 d post-weaning on pig growth performance and weaning associated intestinal dysfunction.
31640819	2	42	from	effects	493:499	arg1	performance					598:608	pig growth performance	587:608	pig growth performance	587:608	The objective of this experiment was to determine the effects of increasing dietary inclusion levels of laminarin in the first 14 d post-weaning on pig growth performance and weaning associated intestinal dysfunction.
31640819	4	43	theme	%	1029:1029	arg1	extract					1017:1023	a laminarin-rich extract	1000:1023	a laminarin-rich extract (65 % laminarin)	1000:1040	The appropriate quantity of a laminarin-rich extract (65 % laminarin) was added to the basal diet to achieve the above dietary inclusion levels of laminarin.
31640819	4	43	theme	%	1029:1029	arg1	laminarin					1031:1039	65 % laminarin	1026:1039	65 % laminarin	1026:1039	The appropriate quantity of a laminarin-rich extract (65 % laminarin) was added to the basal diet to achieve the above dietary inclusion levels of laminarin.
31640819	5	44	theme	supplementation	1144:1158	arg1	d					1139:1139	14 d	1136:1139	14 d of supplementation	1136:1158	After 14 d of supplementation, eight pigs from the basal group and the best-performing laminarin group were euthanised for sample collection.
31640819	0	45	theme	Laminarin-rich	0:13	arg1	extract					15:21	Laminarin-rich extract	0:21	Laminarin-rich extract	0:21	Laminarin-rich extract improves growth performance, small intestinal morphology, gene expression of nutrient transporters and the large intestinal microbial composition of piglets during the critical post-weaning period.
31640819	1	46	theme	growth	306:311	arg1	check					313:317	the post-weaning growth check	289:317	the post-weaning growth check	289:317	The identification of natural bioactive compounds which can prevent the post-weaning growth check and enhance gastrointestinal health in the absence of in-feed medications is an urgent priority for the swine industry.
31640819	3	47	theme	basal	928:932	arg1	laminarin					944:952	(4) basal + 300 ppm laminarin	924:952	(4) basal + 300 ppm laminarin	924:952	At weaning, ninety-six pigs (8·4 (sd 1·09) kg) (meatline boars × (large white × landrace sows)) were blocked by live weight, litter and sex and randomly assigned to: (1) basal diet; (2) basal + 100 parts per million (ppm) laminarin; (3) basal + 200 ppm laminarin and (4) basal + 300 ppm laminarin (three pigs/pen).
31640819	6	48	theme	basal	1398:1402	arg1	group					1404:1408	the basal group	1394:1408	the basal group (P < 0·05)	1394:1419	The 300 ppm laminarin group was selected as this group had higher ADFI compared with all other groups and higher ADG than the basal group (P < 0·05).
31640819	6	48	theme	basal	1398:1402	arg1	0·05					1415:1418	P < 0·05	1411:1418	P < 0·05	1411:1418	The 300 ppm laminarin group was selected as this group had higher ADFI compared with all other groups and higher ADG than the basal group (P < 0·05).
31640819	3	49	theme	basal	843:847	arg1	diet					833:836	(1) basal diet	823:836	(1) basal diet	823:836	At weaning, ninety-six pigs (8·4 (sd 1·09) kg) (meatline boars × (large white × landrace sows)) were blocked by live weight, litter and sex and randomly assigned to: (1) basal diet; (2) basal + 100 parts per million (ppm) laminarin; (3) basal + 200 ppm laminarin and (4) basal + 300 ppm laminarin (three pigs/pen).
31640819	2	50	theme	experiment	461:470	arg1	objective					443:451	The objective	439:451	The objective of this experiment	439:470	The objective of this experiment was to determine the effects of increasing dietary inclusion levels of laminarin in the first 14 d post-weaning on pig growth performance and weaning associated intestinal dysfunction.
31640819	10	51	from	extract	1986:1992	arg1	laminarin					1954:1962	300 ppm laminarin	1946:1962	300 ppm laminarin from a laminarin-rich extract	1946:1992	In conclusion, 300 ppm laminarin from a laminarin-rich extract has potential, as a dietary supplement, to improve performance and prevent post-weaning intestinal dysfunction.
31640819	9	52	theme	<	1818:1818	arg1	0·05					1820:1823	P < 0·05	1816:1823	P < 0·05	1816:1823	Laminarin supplementation reduced Enterobacteriaceae numbers in the caecum (P < 0·05) and increased lactobacilli numbers (P < 0·05), total volatile fatty acid concentrations and the molar proportions of butyrate (P < 0·01) in the colon.
31640819	9	52	theme	<	1818:1818	arg1	numbers					1807:1813	increased lactobacilli numbers	1784:1813	increased lactobacilli numbers (P < 0·05)	1784:1824	Laminarin supplementation reduced Enterobacteriaceae numbers in the caecum (P < 0·05) and increased lactobacilli numbers (P < 0·05), total volatile fatty acid concentrations and the molar proportions of butyrate (P < 0·01) in the colon.
31640819	9	53	theme	P	1770:1770	arg1	caecum					1762:1767	the caecum	1758:1767	the caecum (P < 0·05)	1758:1778	Laminarin supplementation reduced Enterobacteriaceae numbers in the caecum (P < 0·05) and increased lactobacilli numbers (P < 0·05), total volatile fatty acid concentrations and the molar proportions of butyrate (P < 0·01) in the colon.
31640819	9	53	theme	P	1770:1770	arg1	0·05					1774:1777	P < 0·05	1770:1777	P < 0·05	1770:1777	Laminarin supplementation reduced Enterobacteriaceae numbers in the caecum (P < 0·05) and increased lactobacilli numbers (P < 0·05), total volatile fatty acid concentrations and the molar proportions of butyrate (P < 0·01) in the colon.
31640819	4	54	theme	above	1085:1089	arg1	levels					1109:1114	the above dietary inclusion levels	1081:1114	the above dietary inclusion levels of laminarin	1081:1127	The appropriate quantity of a laminarin-rich extract (65 % laminarin) was added to the basal diet to achieve the above dietary inclusion levels of laminarin.
31640819	9	55	from	proportions	1882:1892	arg1	caecum					1762:1767	the caecum	1758:1767	the caecum (P < 0·05)	1758:1778	Laminarin supplementation reduced Enterobacteriaceae numbers in the caecum (P < 0·05) and increased lactobacilli numbers (P < 0·05), total volatile fatty acid concentrations and the molar proportions of butyrate (P < 0·01) in the colon.
31640819	9	55	from	proportions	1882:1892	arg1	0·05					1774:1777	P < 0·05	1770:1777	P < 0·05	1770:1777	Laminarin supplementation reduced Enterobacteriaceae numbers in the caecum (P < 0·05) and increased lactobacilli numbers (P < 0·05), total volatile fatty acid concentrations and the molar proportions of butyrate (P < 0·01) in the colon.
31640819	1	56	theme	gastrointestinal	331:346	arg1	health					348:353	gastrointestinal health	331:353	gastrointestinal health in the absence of in-feed medications	331:391	The identification of natural bioactive compounds which can prevent the post-weaning growth check and enhance gastrointestinal health in the absence of in-feed medications is an urgent priority for the swine industry.
31640819	3	57	theme	per	861:863	arg1	laminarin					879:887	100 parts per million (ppm) laminarin	851:887	100 parts per million (ppm) laminarin	851:887	At weaning, ninety-six pigs (8·4 (sd 1·09) kg) (meatline boars × (large white × landrace sows)) were blocked by live weight, litter and sex and randomly assigned to: (1) basal diet; (2) basal + 100 parts per million (ppm) laminarin; (3) basal + 200 ppm laminarin and (4) basal + 300 ppm laminarin (three pigs/pen).
31640819	3	58	theme	ninety-six	669:678	arg1	pigs					680:683	ninety-six pigs	669:683	ninety-six pigs (8·4 (sd 1·09) kg) (meatline boars × (large white × landrace sows))	669:751	At weaning, ninety-six pigs (8·4 (sd 1·09) kg) (meatline boars × (large white × landrace sows)) were blocked by live weight, litter and sex and randomly assigned to: (1) basal diet; (2) basal + 100 parts per million (ppm) laminarin; (3) basal + 200 ppm laminarin and (4) basal + 300 ppm laminarin (three pigs/pen).
31640819	3	58	theme	ninety-six	669:678	arg1	8·4					686:688	8·4 (sd 1·09) kg	686:701	8·4 (sd 1·09) kg	686:701	At weaning, ninety-six pigs (8·4 (sd 1·09) kg) (meatline boars × (large white × landrace sows)) were blocked by live weight, litter and sex and randomly assigned to: (1) basal diet; (2) basal + 100 parts per million (ppm) laminarin; (3) basal + 200 ppm laminarin and (4) basal + 300 ppm laminarin (three pigs/pen).
31640819	5	59	theme	basal	1181:1185	arg1	group					1187:1191	the basal group	1177:1191	the basal group	1177:1191	After 14 d of supplementation, eight pigs from the basal group and the best-performing laminarin group were euthanised for sample collection.
31640819	0	60	theme	small	52:56	arg1	morphology					69:78	small intestinal morphology	52:78	small intestinal morphology	52:78	Laminarin-rich extract improves growth performance, small intestinal morphology, gene expression of nutrient transporters and the large intestinal microbial composition of piglets during the critical post-weaning period.
31640819	5	61	from	group	1227:1231	arg1	pigs					1167:1170	eight pigs	1161:1170	eight pigs from the basal group and the best-performing laminarin group	1161:1231	After 14 d of supplementation, eight pigs from the basal group and the best-performing laminarin group were euthanised for sample collection.
31640819	9	62	theme	fatty	1842:1846	arg1	concentrations					1853:1866	total volatile fatty acid concentrations	1827:1866	total volatile fatty acid concentrations	1827:1866	Laminarin supplementation reduced Enterobacteriaceae numbers in the caecum (P < 0·05) and increased lactobacilli numbers (P < 0·05), total volatile fatty acid concentrations and the molar proportions of butyrate (P < 0·01) in the colon.
31640819	1	63	theme	natural	243:249	arg1	compounds					261:269	natural bioactive compounds	243:269	natural bioactive compounds which can prevent the post-weaning growth check and enhance gastrointestinal health in the absence of in-feed medications	243:391	The identification of natural bioactive compounds which can prevent the post-weaning growth check and enhance gastrointestinal health in the absence of in-feed medications is an urgent priority for the swine industry.
31640819	6	64	theme	ppm	1280:1282	arg1	group					1294:1298	The 300 ppm laminarin group	1272:1298	The 300 ppm laminarin group	1272:1298	The 300 ppm laminarin group was selected as this group had higher ADFI compared with all other groups and higher ADG than the basal group (P < 0·05).
31640819	3	65	dep	pigs	680:683	arg1	boars					714:718	meatline boars ×	705:720	meatline boars × (large white × landrace sows)	705:750	At weaning, ninety-six pigs (8·4 (sd 1·09) kg) (meatline boars × (large white × landrace sows)) were blocked by live weight, litter and sex and randomly assigned to: (1) basal diet; (2) basal + 100 parts per million (ppm) laminarin; (3) basal + 200 ppm laminarin and (4) basal + 300 ppm laminarin (three pigs/pen).
31640819	3	65	dep	pigs	680:683	arg1	sows					746:749	large white × landrace sows	723:749	large white × landrace sows	723:749	At weaning, ninety-six pigs (8·4 (sd 1·09) kg) (meatline boars × (large white × landrace sows)) were blocked by live weight, litter and sex and randomly assigned to: (1) basal diet; (2) basal + 100 parts per million (ppm) laminarin; (3) basal + 200 ppm laminarin and (4) basal + 300 ppm laminarin (three pigs/pen).
31640819	8	66	dep	jejunum	1655:1661	arg1	the					1651:1653	the	1651:1653	the	1651:1653	Laminarin supplementation increased the expression of SLC2A8/GLUT8 in the duodenum, SLC2A2/GLUT2, SLC2A7/GLUT7, SLC15A1/PEPT1 and FABP2 in the jejunum and SLC16A1/MCT1 in the colon.
31640819	0	67	theme	critical	191:198	arg1	period					213:218	the critical post-weaning period	187:218	the critical post-weaning period	187:218	Laminarin-rich extract improves growth performance, small intestinal morphology, gene expression of nutrient transporters and the large intestinal microbial composition of piglets during the critical post-weaning period.
31640819	2	68	theme	associated	622:631	arg1	dysfunction					644:654	weaning associated intestinal dysfunction	614:654	weaning associated intestinal dysfunction	614:654	The objective of this experiment was to determine the effects of increasing dietary inclusion levels of laminarin in the first 14 d post-weaning on pig growth performance and weaning associated intestinal dysfunction.
31640819	4	69	theme	inclusion	1099:1107	arg1	levels					1109:1114	the above dietary inclusion levels	1081:1114	the above dietary inclusion levels of laminarin	1081:1127	The appropriate quantity of a laminarin-rich extract (65 % laminarin) was added to the basal diet to achieve the above dietary inclusion levels of laminarin.
31640819	9	70	theme	butyrate	1897:1904	arg1	0·05					1820:1823	P < 0·05	1816:1823	P < 0·05	1816:1823	Laminarin supplementation reduced Enterobacteriaceae numbers in the caecum (P < 0·05) and increased lactobacilli numbers (P < 0·05), total volatile fatty acid concentrations and the molar proportions of butyrate (P < 0·01) in the colon.
31640819	9	70	theme	butyrate	1897:1904	arg1	numbers					1807:1813	increased lactobacilli numbers	1784:1813	increased lactobacilli numbers (P < 0·05)	1784:1824	Laminarin supplementation reduced Enterobacteriaceae numbers in the caecum (P < 0·05) and increased lactobacilli numbers (P < 0·05), total volatile fatty acid concentrations and the molar proportions of butyrate (P < 0·01) in the colon.
31640819	9	70	theme	butyrate	1897:1904	arg1	numbers					1747:1753	Enterobacteriaceae numbers	1728:1753	Enterobacteriaceae numbers in the caecum (P < 0·05)	1728:1778	Laminarin supplementation reduced Enterobacteriaceae numbers in the caecum (P < 0·05) and increased lactobacilli numbers (P < 0·05), total volatile fatty acid concentrations and the molar proportions of butyrate (P < 0·01) in the colon.
31640819	9	70	theme	butyrate	1897:1904	arg1	proportions					1882:1892	the molar proportions	1872:1892	the molar proportions of butyrate	1872:1904	Laminarin supplementation reduced Enterobacteriaceae numbers in the caecum (P < 0·05) and increased lactobacilli numbers (P < 0·05), total volatile fatty acid concentrations and the molar proportions of butyrate (P < 0·01) in the colon.
31640819	9	70	theme	butyrate	1897:1904	arg1	0·01					1911:1914	P < 0·01	1907:1914	P < 0·01	1907:1914	Laminarin supplementation reduced Enterobacteriaceae numbers in the caecum (P < 0·05) and increased lactobacilli numbers (P < 0·05), total volatile fatty acid concentrations and the molar proportions of butyrate (P < 0·01) in the colon.
31640819	9	70	theme	butyrate	1897:1904	arg1	concentrations					1853:1866	total volatile fatty acid concentrations	1827:1866	total volatile fatty acid concentrations	1827:1866	Laminarin supplementation reduced Enterobacteriaceae numbers in the caecum (P < 0·05) and increased lactobacilli numbers (P < 0·05), total volatile fatty acid concentrations and the molar proportions of butyrate (P < 0·01) in the colon.
31640819	5	71	theme	sample	1253:1258	arg1	collection					1260:1269	sample collection	1253:1269	sample collection	1253:1269	After 14 d of supplementation, eight pigs from the basal group and the best-performing laminarin group were euthanised for sample collection.
31640819	10	72	theme	dietary	2014:2020	arg1	potential					1998:2006	potential	1998:2006	potential	1998:2006	In conclusion, 300 ppm laminarin from a laminarin-rich extract has potential, as a dietary supplement, to improve performance and prevent post-weaning intestinal dysfunction.
31640819	10	72	theme	dietary	2014:2020	arg1	supplement					2022:2031	a dietary supplement	2012:2031	a dietary supplement	2012:2031	In conclusion, 300 ppm laminarin from a laminarin-rich extract has potential, as a dietary supplement, to improve performance and prevent post-weaning intestinal dysfunction.
31640819	3	73	theme	200	902:904	arg1	ppm					906:908	ppm	906:908	ppm	906:908	At weaning, ninety-six pigs (8·4 (sd 1·09) kg) (meatline boars × (large white × landrace sows)) were blocked by live weight, litter and sex and randomly assigned to: (1) basal diet; (2) basal + 100 parts per million (ppm) laminarin; (3) basal + 200 ppm laminarin and (4) basal + 300 ppm laminarin (three pigs/pen).
31640819	9	74	theme	P	1907:1907	arg1	0·01					1911:1914	P < 0·01	1907:1914	P < 0·01	1907:1914	Laminarin supplementation reduced Enterobacteriaceae numbers in the caecum (P < 0·05) and increased lactobacilli numbers (P < 0·05), total volatile fatty acid concentrations and the molar proportions of butyrate (P < 0·01) in the colon.
31640819	9	74	theme	P	1907:1907	arg1	numbers					1807:1813	increased lactobacilli numbers	1784:1813	increased lactobacilli numbers (P < 0·05)	1784:1824	Laminarin supplementation reduced Enterobacteriaceae numbers in the caecum (P < 0·05) and increased lactobacilli numbers (P < 0·05), total volatile fatty acid concentrations and the molar proportions of butyrate (P < 0·01) in the colon.
31640819	5	75	theme	best-performing	1201:1215	arg1	group					1227:1231	the best-performing laminarin group	1197:1231	the best-performing laminarin group	1197:1231	After 14 d of supplementation, eight pigs from the basal group and the best-performing laminarin group were euthanised for sample collection.
31640819	3	76	theme	basal	894:898	arg1	diet					833:836	(1) basal diet	823:836	(1) basal diet	823:836	At weaning, ninety-six pigs (8·4 (sd 1·09) kg) (meatline boars × (large white × landrace sows)) were blocked by live weight, litter and sex and randomly assigned to: (1) basal diet; (2) basal + 100 parts per million (ppm) laminarin; (3) basal + 200 ppm laminarin and (4) basal + 300 ppm laminarin (three pigs/pen).
31640819	9	77	theme	Laminarin	1694:1702	arg1	supplementation					1704:1718	Laminarin supplementation	1694:1718	Laminarin supplementation	1694:1718	Laminarin supplementation reduced Enterobacteriaceae numbers in the caecum (P < 0·05) and increased lactobacilli numbers (P < 0·05), total volatile fatty acid concentrations and the molar proportions of butyrate (P < 0·01) in the colon.
31640819	3	78	theme	landrace	737:744	arg1	boars					714:718	meatline boars ×	705:720	meatline boars × (large white × landrace sows)	705:750	At weaning, ninety-six pigs (8·4 (sd 1·09) kg) (meatline boars × (large white × landrace sows)) were blocked by live weight, litter and sex and randomly assigned to: (1) basal diet; (2) basal + 100 parts per million (ppm) laminarin; (3) basal + 200 ppm laminarin and (4) basal + 300 ppm laminarin (three pigs/pen).
31640819	3	78	theme	landrace	737:744	arg1	sows					746:749	large white × landrace sows	723:749	large white × landrace sows	723:749	At weaning, ninety-six pigs (8·4 (sd 1·09) kg) (meatline boars × (large white × landrace sows)) were blocked by live weight, litter and sex and randomly assigned to: (1) basal diet; (2) basal + 100 parts per million (ppm) laminarin; (3) basal + 200 ppm laminarin and (4) basal + 300 ppm laminarin (three pigs/pen).
31640819	0	79	theme	large	130:134	arg1	composition					157:167	the large intestinal microbial composition	126:167	the large intestinal microbial composition of piglets	126:178	Laminarin-rich extract improves growth performance, small intestinal morphology, gene expression of nutrient transporters and the large intestinal microbial composition of piglets during the critical post-weaning period.
31640819	9	80	theme	<	1909:1909	arg1	0·01					1911:1914	P < 0·01	1907:1914	P < 0·01	1907:1914	Laminarin supplementation reduced Enterobacteriaceae numbers in the caecum (P < 0·05) and increased lactobacilli numbers (P < 0·05), total volatile fatty acid concentrations and the molar proportions of butyrate (P < 0·01) in the colon.
31640819	9	80	theme	<	1909:1909	arg1	numbers					1807:1813	increased lactobacilli numbers	1784:1813	increased lactobacilli numbers (P < 0·05)	1784:1824	Laminarin supplementation reduced Enterobacteriaceae numbers in the caecum (P < 0·05) and increased lactobacilli numbers (P < 0·05), total volatile fatty acid concentrations and the molar proportions of butyrate (P < 0·01) in the colon.
31640819	3	81	theme	white	729:733	arg1	boars					714:718	meatline boars ×	705:720	meatline boars × (large white × landrace sows)	705:750	At weaning, ninety-six pigs (8·4 (sd 1·09) kg) (meatline boars × (large white × landrace sows)) were blocked by live weight, litter and sex and randomly assigned to: (1) basal diet; (2) basal + 100 parts per million (ppm) laminarin; (3) basal + 200 ppm laminarin and (4) basal + 300 ppm laminarin (three pigs/pen).
31640819	3	81	theme	white	729:733	arg1	sows					746:749	large white × landrace sows	723:749	large white × landrace sows	723:749	At weaning, ninety-six pigs (8·4 (sd 1·09) kg) (meatline boars × (large white × landrace sows)) were blocked by live weight, litter and sex and randomly assigned to: (1) basal diet; (2) basal + 100 parts per million (ppm) laminarin; (3) basal + 200 ppm laminarin and (4) basal + 300 ppm laminarin (three pigs/pen).
31640819	0	82	theme	microbial	147:155	arg1	composition					157:167	the large intestinal microbial composition	126:167	the large intestinal microbial composition of piglets	126:178	Laminarin-rich extract improves growth performance, small intestinal morphology, gene expression of nutrient transporters and the large intestinal microbial composition of piglets during the critical post-weaning period.
31640819	2	83	theme	first	560:564	arg1	d					569:569	the first 14 d	556:569	the first 14 d	556:569	The objective of this experiment was to determine the effects of increasing dietary inclusion levels of laminarin in the first 14 d post-weaning on pig growth performance and weaning associated intestinal dysfunction.
31640819	4	84	theme	extract	1017:1023	arg1	quantity					988:995	The appropriate quantity	972:995	The appropriate quantity of a laminarin-rich extract (65 % laminarin)	972:1040	The appropriate quantity of a laminarin-rich extract (65 % laminarin) was added to the basal diet to achieve the above dietary inclusion levels of laminarin.
31640819	3	85	dep	diet	833:836	arg1	1					824:824	1	824:824	1	824:824	At weaning, ninety-six pigs (8·4 (sd 1·09) kg) (meatline boars × (large white × landrace sows)) were blocked by live weight, litter and sex and randomly assigned to: (1) basal diet; (2) basal + 100 parts per million (ppm) laminarin; (3) basal + 200 ppm laminarin and (4) basal + 300 ppm laminarin (three pigs/pen).
31640819	2	86	theme	growth	591:596	arg1	performance					598:608	pig growth performance	587:608	pig growth performance	587:608	The objective of this experiment was to determine the effects of increasing dietary inclusion levels of laminarin in the first 14 d post-weaning on pig growth performance and weaning associated intestinal dysfunction.
31640819	4	87	theme	65	1026:1027	arg1	%					1029:1029	%	1029:1029	%	1029:1029	The appropriate quantity of a laminarin-rich extract (65 % laminarin) was added to the basal diet to achieve the above dietary inclusion levels of laminarin.
31640819	9	88	theme	total	1827:1831	arg1	concentrations					1853:1866	total volatile fatty acid concentrations	1827:1866	total volatile fatty acid concentrations	1827:1866	Laminarin supplementation reduced Enterobacteriaceae numbers in the caecum (P < 0·05) and increased lactobacilli numbers (P < 0·05), total volatile fatty acid concentrations and the molar proportions of butyrate (P < 0·01) in the colon.
31640819	6	89	theme	other	1361:1365	arg1	groups					1367:1372	all other groups	1357:1372	all other groups	1357:1372	The 300 ppm laminarin group was selected as this group had higher ADFI compared with all other groups and higher ADG than the basal group (P < 0·05).
31640819	7	90	theme	<	1503:1503	arg1	duodenum					1479:1486	duodenum	1479:1486	duodenum	1479:1486	Laminarin supplementation increased villus height in the duodenum and jejunum (P < 0·05).
31640819	7	90	theme	<	1503:1503	arg1	0·05					1505:1508	P < 0·05	1501:1508	P < 0·05	1501:1508	Laminarin supplementation increased villus height in the duodenum and jejunum (P < 0·05).
31640819	2	91	theme	inclusion	523:531	arg1	levels					533:538	dietary inclusion levels	515:538	dietary inclusion levels of laminarin	515:551	The objective of this experiment was to determine the effects of increasing dietary inclusion levels of laminarin in the first 14 d post-weaning on pig growth performance and weaning associated intestinal dysfunction.
31640819	9	92	theme	<	1772:1772	arg1	caecum					1762:1767	the caecum	1758:1767	the caecum (P < 0·05)	1758:1778	Laminarin supplementation reduced Enterobacteriaceae numbers in the caecum (P < 0·05) and increased lactobacilli numbers (P < 0·05), total volatile fatty acid concentrations and the molar proportions of butyrate (P < 0·01) in the colon.
31640819	9	92	theme	<	1772:1772	arg1	0·05					1774:1777	P < 0·05	1770:1777	P < 0·05	1770:1777	Laminarin supplementation reduced Enterobacteriaceae numbers in the caecum (P < 0·05) and increased lactobacilli numbers (P < 0·05), total volatile fatty acid concentrations and the molar proportions of butyrate (P < 0·01) in the colon.
31640819	6	93	contain	had	1327:1329	arg1	group					1321:1325	this group	1316:1325	this group	1316:1325	The 300 ppm laminarin group was selected as this group had higher ADFI compared with all other groups and higher ADG than the basal group (P < 0·05).
31640819	6	93	contain	had	1327:1329	arg2	ADFI					1338:1341	higher ADFI	1331:1341	higher ADFI	1331:1341	The 300 ppm laminarin group was selected as this group had higher ADFI compared with all other groups and higher ADG than the basal group (P < 0·05).
31640819	10	94	theme	ppm	1950:1952	arg1	laminarin					1954:1962	300 ppm laminarin	1946:1962	300 ppm laminarin from a laminarin-rich extract	1946:1992	In conclusion, 300 ppm laminarin from a laminarin-rich extract has potential, as a dietary supplement, to improve performance and prevent post-weaning intestinal dysfunction.
31640819	0	95	theme	nutrient	100:107	arg1	transporters					109:120	nutrient transporters	100:120	nutrient transporters	100:120	Laminarin-rich extract improves growth performance, small intestinal morphology, gene expression of nutrient transporters and the large intestinal microbial composition of piglets during the critical post-weaning period.
31640819	1	96	theme	medications	381:391	arg1	absence					362:368	the absence	358:368	the absence of in-feed medications	358:391	The identification of natural bioactive compounds which can prevent the post-weaning growth check and enhance gastrointestinal health in the absence of in-feed medications is an urgent priority for the swine industry.
31640819	9	97	theme	increased	1784:1792	arg1	0·05					1820:1823	P < 0·05	1816:1823	P < 0·05	1816:1823	Laminarin supplementation reduced Enterobacteriaceae numbers in the caecum (P < 0·05) and increased lactobacilli numbers (P < 0·05), total volatile fatty acid concentrations and the molar proportions of butyrate (P < 0·01) in the colon.
31640819	9	97	theme	increased	1784:1792	arg1	0·01					1911:1914	P < 0·01	1907:1914	P < 0·01	1907:1914	Laminarin supplementation reduced Enterobacteriaceae numbers in the caecum (P < 0·05) and increased lactobacilli numbers (P < 0·05), total volatile fatty acid concentrations and the molar proportions of butyrate (P < 0·01) in the colon.
31640819	9	97	theme	increased	1784:1792	arg1	numbers					1807:1813	increased lactobacilli numbers	1784:1813	increased lactobacilli numbers (P < 0·05)	1784:1824	Laminarin supplementation reduced Enterobacteriaceae numbers in the caecum (P < 0·05) and increased lactobacilli numbers (P < 0·05), total volatile fatty acid concentrations and the molar proportions of butyrate (P < 0·01) in the colon.
31640819	4	98	theme	basal	1059:1063	arg1	diet					1065:1068	the basal diet to achieve the above dietary inclusion levels of laminarin	1055:1127	the basal diet to achieve the above dietary inclusion levels of laminarin	1055:1127	The appropriate quantity of a laminarin-rich extract (65 % laminarin) was added to the basal diet to achieve the above dietary inclusion levels of laminarin.
31640819	6	99	theme	P	1411:1411	arg1	group					1404:1408	the basal group	1394:1408	the basal group (P < 0·05)	1394:1419	The 300 ppm laminarin group was selected as this group had higher ADFI compared with all other groups and higher ADG than the basal group (P < 0·05).
31640819	6	99	theme	P	1411:1411	arg1	0·05					1415:1418	P < 0·05	1411:1418	P < 0·05	1411:1418	The 300 ppm laminarin group was selected as this group had higher ADFI compared with all other groups and higher ADG than the basal group (P < 0·05).
31640819	10	100	theme	laminarin-rich	1971:1984	arg1	extract					1986:1992	a laminarin-rich extract	1969:1992	a laminarin-rich extract	1969:1992	In conclusion, 300 ppm laminarin from a laminarin-rich extract has potential, as a dietary supplement, to improve performance and prevent post-weaning intestinal dysfunction.
31640819	10	101	contain	has	1994:1996	arg2	supplement					2022:2031	a dietary supplement	2012:2031	a dietary supplement	2012:2031	In conclusion, 300 ppm laminarin from a laminarin-rich extract has potential, as a dietary supplement, to improve performance and prevent post-weaning intestinal dysfunction.
31640819	10	101	contain	has	1994:1996	arg1	laminarin					1954:1962	300 ppm laminarin	1946:1962	300 ppm laminarin from a laminarin-rich extract	1946:1992	In conclusion, 300 ppm laminarin from a laminarin-rich extract has potential, as a dietary supplement, to improve performance and prevent post-weaning intestinal dysfunction.
31640819	10	101	contain	has	1994:1996	arg2	potential					1998:2006	potential	1998:2006	potential	1998:2006	In conclusion, 300 ppm laminarin from a laminarin-rich extract has potential, as a dietary supplement, to improve performance and prevent post-weaning intestinal dysfunction.
31640819	9	102	theme	P	1816:1816	arg1	0·05					1820:1823	P < 0·05	1816:1823	P < 0·05	1816:1823	Laminarin supplementation reduced Enterobacteriaceae numbers in the caecum (P < 0·05) and increased lactobacilli numbers (P < 0·05), total volatile fatty acid concentrations and the molar proportions of butyrate (P < 0·01) in the colon.
31640819	9	102	theme	P	1816:1816	arg1	numbers					1807:1813	increased lactobacilli numbers	1784:1813	increased lactobacilli numbers (P < 0·05)	1784:1824	Laminarin supplementation reduced Enterobacteriaceae numbers in the caecum (P < 0·05) and increased lactobacilli numbers (P < 0·05), total volatile fatty acid concentrations and the molar proportions of butyrate (P < 0·01) in the colon.
31640819	0	103	theme	growth	32:37	arg1	performance					39:49	growth performance	32:49	growth performance	32:49	Laminarin-rich extract improves growth performance, small intestinal morphology, gene expression of nutrient transporters and the large intestinal microbial composition of piglets during the critical post-weaning period.
31640819	5	104	from	group	1187:1191	arg1	pigs					1167:1170	eight pigs	1161:1170	eight pigs from the basal group and the best-performing laminarin group	1161:1231	After 14 d of supplementation, eight pigs from the basal group and the best-performing laminarin group were euthanised for sample collection.
31640819	4	105	theme	dietary	1091:1097	arg1	levels					1109:1114	the above dietary inclusion levels	1081:1114	the above dietary inclusion levels of laminarin	1081:1127	The appropriate quantity of a laminarin-rich extract (65 % laminarin) was added to the basal diet to achieve the above dietary inclusion levels of laminarin.
31640819	3	106	theme	ppm	940:942	arg1	laminarin					944:952	(4) basal + 300 ppm laminarin	924:952	(4) basal + 300 ppm laminarin	924:952	At weaning, ninety-six pigs (8·4 (sd 1·09) kg) (meatline boars × (large white × landrace sows)) were blocked by live weight, litter and sex and randomly assigned to: (1) basal diet; (2) basal + 100 parts per million (ppm) laminarin; (3) basal + 200 ppm laminarin and (4) basal + 300 ppm laminarin (three pigs/pen).
31640819	7	107	theme	Laminarin	1422:1430	arg1	supplementation					1432:1446	Laminarin supplementation	1422:1446	Laminarin supplementation	1422:1446	Laminarin supplementation increased villus height in the duodenum and jejunum (P < 0·05).
31640819	9	108	from	numbers	1747:1753	arg1	caecum					1762:1767	the caecum	1758:1767	the caecum (P < 0·05)	1758:1778	Laminarin supplementation reduced Enterobacteriaceae numbers in the caecum (P < 0·05) and increased lactobacilli numbers (P < 0·05), total volatile fatty acid concentrations and the molar proportions of butyrate (P < 0·01) in the colon.
31640819	9	108	from	numbers	1747:1753	arg1	0·05					1774:1777	P < 0·05	1770:1777	P < 0·05	1770:1777	Laminarin supplementation reduced Enterobacteriaceae numbers in the caecum (P < 0·05) and increased lactobacilli numbers (P < 0·05), total volatile fatty acid concentrations and the molar proportions of butyrate (P < 0·01) in the colon.
31640819	8	109	from	SLC16A1/MCT1	1667:1678	arg1	colon					1687:1691	the colon	1683:1691	the colon	1683:1691	Laminarin supplementation increased the expression of SLC2A8/GLUT8 in the duodenum, SLC2A2/GLUT2, SLC2A7/GLUT7, SLC15A1/PEPT1 and FABP2 in the jejunum and SLC16A1/MCT1 in the colon.
31640819	1	110	theme	compounds	261:269	arg1	identification					225:238	The identification	221:238	The identification of natural bioactive compounds which can prevent the post-weaning growth check and enhance gastrointestinal health in the absence of in-feed medications	221:391	The identification of natural bioactive compounds which can prevent the post-weaning growth check and enhance gastrointestinal health in the absence of in-feed medications is an urgent priority for the swine industry.
31640819	1	110	theme	compounds	261:269	arg1	priority					406:413	an urgent priority	396:413	an urgent priority for the swine industry	396:436	The identification of natural bioactive compounds which can prevent the post-weaning growth check and enhance gastrointestinal health in the absence of in-feed medications is an urgent priority for the swine industry.
31640819	0	111	theme	intestinal	58:67	arg1	morphology					69:78	small intestinal morphology	52:78	small intestinal morphology	52:78	Laminarin-rich extract improves growth performance, small intestinal morphology, gene expression of nutrient transporters and the large intestinal microbial composition of piglets during the critical post-weaning period.
31640819	3	112	theme	million	865:871	arg1	laminarin					879:887	100 parts per million (ppm) laminarin	851:887	100 parts per million (ppm) laminarin	851:887	At weaning, ninety-six pigs (8·4 (sd 1·09) kg) (meatline boars × (large white × landrace sows)) were blocked by live weight, litter and sex and randomly assigned to: (1) basal diet; (2) basal + 100 parts per million (ppm) laminarin; (3) basal + 200 ppm laminarin and (4) basal + 300 ppm laminarin (three pigs/pen).
31640819	8	113	from	jejunum	1655:1661	arg1	colon					1687:1691	the colon	1683:1691	the colon	1683:1691	Laminarin supplementation increased the expression of SLC2A8/GLUT8 in the duodenum, SLC2A2/GLUT2, SLC2A7/GLUT7, SLC15A1/PEPT1 and FABP2 in the jejunum and SLC16A1/MCT1 in the colon.
31640819	9	114	theme	volatile	1833:1840	arg1	concentrations					1853:1866	total volatile fatty acid concentrations	1827:1866	total volatile fatty acid concentrations	1827:1866	Laminarin supplementation reduced Enterobacteriaceae numbers in the caecum (P < 0·05) and increased lactobacilli numbers (P < 0·05), total volatile fatty acid concentrations and the molar proportions of butyrate (P < 0·01) in the colon.
31640819	3	115	theme	parts	855:859	arg1	laminarin					879:887	100 parts per million (ppm) laminarin	851:887	100 parts per million (ppm) laminarin	851:887	At weaning, ninety-six pigs (8·4 (sd 1·09) kg) (meatline boars × (large white × landrace sows)) were blocked by live weight, litter and sex and randomly assigned to: (1) basal diet; (2) basal + 100 parts per million (ppm) laminarin; (3) basal + 200 ppm laminarin and (4) basal + 300 ppm laminarin (three pigs/pen).
31640819	9	116	from	numbers	1807:1813	arg1	caecum					1762:1767	the caecum	1758:1767	the caecum (P < 0·05)	1758:1778	Laminarin supplementation reduced Enterobacteriaceae numbers in the caecum (P < 0·05) and increased lactobacilli numbers (P < 0·05), total volatile fatty acid concentrations and the molar proportions of butyrate (P < 0·01) in the colon.
31640819	9	116	from	numbers	1807:1813	arg1	0·05					1774:1777	P < 0·05	1770:1777	P < 0·05	1770:1777	Laminarin supplementation reduced Enterobacteriaceae numbers in the caecum (P < 0·05) and increased lactobacilli numbers (P < 0·05), total volatile fatty acid concentrations and the molar proportions of butyrate (P < 0·01) in the colon.
31640819	7	117	theme	villus	1458:1463	arg1	height					1465:1470	villus height	1458:1470	villus height	1458:1470	Laminarin supplementation increased villus height in the duodenum and jejunum (P < 0·05).
31640819	9	118	theme	acid	1848:1851	arg1	concentrations					1853:1866	total volatile fatty acid concentrations	1827:1866	total volatile fatty acid concentrations	1827:1866	Laminarin supplementation reduced Enterobacteriaceae numbers in the caecum (P < 0·05) and increased lactobacilli numbers (P < 0·05), total volatile fatty acid concentrations and the molar proportions of butyrate (P < 0·01) in the colon.
31640819	6	119	theme	laminarin	1284:1292	arg1	group					1294:1298	The 300 ppm laminarin group	1272:1298	The 300 ppm laminarin group	1272:1298	The 300 ppm laminarin group was selected as this group had higher ADFI compared with all other groups and higher ADG than the basal group (P < 0·05).
31640819	1	120	theme	bioactive	251:259	arg1	compounds					261:269	natural bioactive compounds	243:269	natural bioactive compounds which can prevent the post-weaning growth check and enhance gastrointestinal health in the absence of in-feed medications	243:391	The identification of natural bioactive compounds which can prevent the post-weaning growth check and enhance gastrointestinal health in the absence of in-feed medications is an urgent priority for the swine industry.
31640819	6	121	theme	300	1276:1278	arg1	ppm					1280:1282	ppm	1280:1282	ppm	1280:1282	The 300 ppm laminarin group was selected as this group had higher ADFI compared with all other groups and higher ADG than the basal group (P < 0·05).
29298541	8	0	theme	fragmenting	979:989	arg1	DNA					991:993	fragmenting DNA	979:993	fragmenting DNA	979:993	Furthermore, cells treated with CS/PLA-PIC NPs showed fragmenting DNA and also demonstrated for apoptotic cells percentage by flow cytometry.
29298541	8	1	theme	apoptotic	1021:1029	arg1	cells					1031:1035	apoptotic cells	1021:1035	apoptotic cells	1021:1035	Furthermore, cells treated with CS/PLA-PIC NPs showed fragmenting DNA and also demonstrated for apoptotic cells percentage by flow cytometry.
29298541	0	2	theme	in	70:71	arg1	activity					89:96	an in vitro apoptosis activity	67:96	an in vitro apoptosis activity	67:96	Chitosan/poly (lactic acid)-coated piceatannol nanoparticles exert an in vitro apoptosis activity on liver, lung and breast cancer cell lines.
29298541	4	3	theme	continuous	598:607	arg1	release					609:615	a continuous release	596:615	a continuous release of the entrapped PIC	596:636	The in vitro drug release study showed that NPs provided a continuous release of the entrapped PIC.
29298541	6	4	theme	cytotoxic	742:750	arg1	efficacy					752:759	higher cytotoxic efficacy	735:759	higher cytotoxic efficacy of CS/PLA-PIC NPs in HepG2, A549 and MCF7 cells	735:807	MTT and LDH assays revealed higher cytotoxic efficacy of CS/PLA-PIC NPs in HepG2, A549 and MCF7 cells compared to CS-PLA NPs and PIC.
29298541	3	5	theme	stability	434:442	arg1	studies					444:450	The preliminary and stability studies	414:450	studies	444:450	The preliminary and stability studies were determined for the polymers drug-loading capacity and encapsulation efficiencies.
29298541	0	6	theme	apoptosis	79:87	arg1	activity					89:96	an in vitro apoptosis activity	67:96	an in vitro apoptosis activity	67:96	Chitosan/poly (lactic acid)-coated piceatannol nanoparticles exert an in vitro apoptosis activity on liver, lung and breast cancer cell lines.
29298541	6	7	theme	NPs	775:777	arg1	efficacy					752:759	higher cytotoxic efficacy	735:759	higher cytotoxic efficacy of CS/PLA-PIC NPs in HepG2, A549 and MCF7 cells	735:807	MTT and LDH assays revealed higher cytotoxic efficacy of CS/PLA-PIC NPs in HepG2, A549 and MCF7 cells compared to CS-PLA NPs and PIC.
29298541	0	8	theme	liver	101:105	arg1	lines					136:140	liver, lung and breast cancer cell lines	101:140	liver, lung and breast cancer cell lines	101:140	Chitosan/poly (lactic acid)-coated piceatannol nanoparticles exert an in vitro apoptosis activity on liver, lung and breast cancer cell lines.
29298541	6	9	theme	CS/PLA-PIC	764:773	arg1	NPs					775:777	CS/PLA-PIC NPs	764:777	CS/PLA-PIC NPs	764:777	MTT and LDH assays revealed higher cytotoxic efficacy of CS/PLA-PIC NPs in HepG2, A549 and MCF7 cells compared to CS-PLA NPs and PIC.
29298541	3	10	theme	preliminary	418:428	arg1	studies					444:450	The preliminary and stability studies	414:450	studies	444:450	The preliminary and stability studies were determined for the polymers drug-loading capacity and encapsulation efficiencies.
29298541	7	11	theme	staining	846:853	arg1	results					855:861	Dual staining results	841:861	Dual staining results	841:861	Dual staining results showed the early/late-stages of apoptotic and necrotic cells.
29298541	2	12	theme	CS/PLA-PIC	378:387	arg1	nanoparticles					363:375	polymeric-drug nanoparticles	348:375	polymeric-drug nanoparticles (CS/PLA-PIC NPs)	348:392	The synthesized nanoparticles form the composite of polymeric-drug nanoparticles (CS/PLA-PIC NPs) by dropping method.
29298541	2	12	theme	CS/PLA-PIC	378:387	arg1	NPs					389:391	CS/PLA-PIC NPs	378:391	CS/PLA-PIC NPs	378:391	The synthesized nanoparticles form the composite of polymeric-drug nanoparticles (CS/PLA-PIC NPs) by dropping method.
29298541	9	13	theme	CS/PLA-PIC	1101:1110	arg1	exposure					1116:1123	CS/PLA-PIC NPs exposure	1101:1123	CS/PLA-PIC NPs exposure	1101:1123	These results suggested that upon CS/PLA-PIC NPs exposure leads to decrease in cancer cell viability due to apoptosis.
29298541	7	14	theme	necrotic	909:916	arg1	cells					918:922	apoptotic and necrotic cells	895:922	apoptotic and necrotic cells	895:922	Dual staining results showed the early/late-stages of apoptotic and necrotic cells.
29298541	9	15	from	decrease	1134:1141	arg1	viability					1158:1166	cancer cell viability	1146:1166	cancer cell viability	1146:1166	These results suggested that upon CS/PLA-PIC NPs exposure leads to decrease in cancer cell viability due to apoptosis.
29298541	0	16	theme	Chitosan/poly	0:12	arg1	nanoparticles					47:59	Chitosan/poly (lactic acid)-coated piceatannol nanoparticles	0:59	Chitosan/poly (lactic acid)-coated piceatannol nanoparticles	0:59	Chitosan/poly (lactic acid)-coated piceatannol nanoparticles exert an in vitro apoptosis activity on liver, lung and breast cancer cell lines.
29298541	0	17	dep	in	70:71	arg1	vitro					73:77	vitro	73:77	vitro	73:77	Chitosan/poly (lactic acid)-coated piceatannol nanoparticles exert an in vitro apoptosis activity on liver, lung and breast cancer cell lines.
29298541	9	18	theme	NPs	1112:1114	arg1	exposure					1116:1123	CS/PLA-PIC NPs exposure	1101:1123	CS/PLA-PIC NPs exposure	1101:1123	These results suggested that upon CS/PLA-PIC NPs exposure leads to decrease in cancer cell viability due to apoptosis.
29298541	5	19	theme	NO	694:695	arg1	radicals					697:704	NO radicals	694:704	NO radicals	694:704	The NPs found to be a good scavenger for DPPH, SOD and NO radicals.
29298541	0	20	theme	lung	108:111	arg1	lines					136:140	liver, lung and breast cancer cell lines	101:140	liver, lung and breast cancer cell lines	101:140	Chitosan/poly (lactic acid)-coated piceatannol nanoparticles exert an in vitro apoptosis activity on liver, lung and breast cancer cell lines.
29298541	2	21	theme	synthesized	300:310	arg1	nanoparticles					312:324	The synthesized nanoparticles	296:324	The synthesized nanoparticles	296:324	The synthesized nanoparticles form the composite of polymeric-drug nanoparticles (CS/PLA-PIC NPs) by dropping method.
29298541	0	22	theme	lactic	15:20	arg1	Chitosan/poly					0:12	Chitosan/poly	0:12	Chitosan/poly (lactic acid)-coated piceatannol nanoparticles	0:59	Chitosan/poly (lactic acid)-coated piceatannol nanoparticles exert an in vitro apoptosis activity on liver, lung and breast cancer cell lines.
29298541	0	22	theme	lactic	15:20	arg1	acid					22:25	lactic acid	15:25	lactic acid	15:25	Chitosan/poly (lactic acid)-coated piceatannol nanoparticles exert an in vitro apoptosis activity on liver, lung and breast cancer cell lines.
29298541	6	23	theme	CS-PLA	821:826	arg1	NPs					828:830	CS-PLA NPs	821:830	CS-PLA NPs	821:830	MTT and LDH assays revealed higher cytotoxic efficacy of CS/PLA-PIC NPs in HepG2, A549 and MCF7 cells compared to CS-PLA NPs and PIC.
29298541	1	24	theme	drug	272:275	arg1	PIC					290:292	PIC	290:292	PIC	290:292	The aim of the study was to synthesize nanoparticles (NPs) with chitosan (CS), and poly (lactic acid) (PLA) as a carrier for the drug piceatannol (PIC).
29298541	1	24	theme	drug	272:275	arg1	piceatannol					277:287	the drug piceatannol	268:287	the drug piceatannol (PIC)	268:293	The aim of the study was to synthesize nanoparticles (NPs) with chitosan (CS), and poly (lactic acid) (PLA) as a carrier for the drug piceatannol (PIC).
29298541	4	25	theme	in	543:544	arg1	study					565:569	The in vitro drug release study	539:569	The in vitro drug release study	539:569	The in vitro drug release study showed that NPs provided a continuous release of the entrapped PIC.
29298541	0	26	theme	-coated	27:33	arg1	nanoparticles					47:59	Chitosan/poly (lactic acid)-coated piceatannol nanoparticles	0:59	Chitosan/poly (lactic acid)-coated piceatannol nanoparticles	0:59	Chitosan/poly (lactic acid)-coated piceatannol nanoparticles exert an in vitro apoptosis activity on liver, lung and breast cancer cell lines.
29298541	0	27	theme	breast	117:122	arg1	lines					136:140	liver, lung and breast cancer cell lines	101:140	liver, lung and breast cancer cell lines	101:140	Chitosan/poly (lactic acid)-coated piceatannol nanoparticles exert an in vitro apoptosis activity on liver, lung and breast cancer cell lines.
29298541	6	28	theme	higher	735:740	arg1	efficacy					752:759	higher cytotoxic efficacy	735:759	higher cytotoxic efficacy of CS/PLA-PIC NPs in HepG2, A549 and MCF7 cells	735:807	MTT and LDH assays revealed higher cytotoxic efficacy of CS/PLA-PIC NPs in HepG2, A549 and MCF7 cells compared to CS-PLA NPs and PIC.
29298541	4	29	theme	release	557:563	arg1	study					565:569	The in vitro drug release study	539:569	The in vitro drug release study	539:569	The in vitro drug release study showed that NPs provided a continuous release of the entrapped PIC.
29298541	2	30	theme	nanoparticles	363:375	arg1	composite					335:343	the composite	331:343	the composite of polymeric-drug nanoparticles (CS/PLA-PIC NPs)	331:392	The synthesized nanoparticles form the composite of polymeric-drug nanoparticles (CS/PLA-PIC NPs) by dropping method.
29298541	2	30	theme	nanoparticles	363:375	arg1	nanoparticles					363:375	polymeric-drug nanoparticles	348:375	polymeric-drug nanoparticles (CS/PLA-PIC NPs)	348:392	The synthesized nanoparticles form the composite of polymeric-drug nanoparticles (CS/PLA-PIC NPs) by dropping method.
29298541	5	31	theme	good	661:664	arg1	NPs					643:645	The NPs	639:645	The NPs	639:645	The NPs found to be a good scavenger for DPPH, SOD and NO radicals.
29298541	5	31	theme	good	661:664	arg1	scavenger					666:674	a good scavenger	659:674	a good scavenger for DPPH, SOD and NO radicals	659:704	The NPs found to be a good scavenger for DPPH, SOD and NO radicals.
29298541	4	32	theme	drug	552:555	arg1	study					565:569	The in vitro drug release study	539:569	The in vitro drug release study	539:569	The in vitro drug release study showed that NPs provided a continuous release of the entrapped PIC.
29298541	2	33	theme	polymeric-drug	348:361	arg1	nanoparticles					363:375	polymeric-drug nanoparticles	348:375	polymeric-drug nanoparticles (CS/PLA-PIC NPs)	348:392	The synthesized nanoparticles form the composite of polymeric-drug nanoparticles (CS/PLA-PIC NPs) by dropping method.
29298541	2	33	theme	polymeric-drug	348:361	arg1	NPs					389:391	CS/PLA-PIC NPs	378:391	CS/PLA-PIC NPs	378:391	The synthesized nanoparticles form the composite of polymeric-drug nanoparticles (CS/PLA-PIC NPs) by dropping method.
29298541	1	34	theme	lactic	232:237	arg1	poly					226:229	poly	226:229	poly (lactic acid) (PLA)	226:249	The aim of the study was to synthesize nanoparticles (NPs) with chitosan (CS), and poly (lactic acid) (PLA) as a carrier for the drug piceatannol (PIC).
29298541	1	34	theme	lactic	232:237	arg1	acid					239:242	lactic acid	232:242	lactic acid	232:242	The aim of the study was to synthesize nanoparticles (NPs) with chitosan (CS), and poly (lactic acid) (PLA) as a carrier for the drug piceatannol (PIC).
29298541	0	35	theme	cell	131:134	arg1	lines					136:140	liver, lung and breast cancer cell lines	101:140	liver, lung and breast cancer cell lines	101:140	Chitosan/poly (lactic acid)-coated piceatannol nanoparticles exert an in vitro apoptosis activity on liver, lung and breast cancer cell lines.
29298541	0	36	theme	piceatannol	35:45	arg1	nanoparticles					47:59	Chitosan/poly (lactic acid)-coated piceatannol nanoparticles	0:59	Chitosan/poly (lactic acid)-coated piceatannol nanoparticles	0:59	Chitosan/poly (lactic acid)-coated piceatannol nanoparticles exert an in vitro apoptosis activity on liver, lung and breast cancer cell lines.
29298541	6	37	theme	MCF7	798:801	arg1	cells					803:807	MCF7 cells	798:807	MCF7 cells	798:807	MTT and LDH assays revealed higher cytotoxic efficacy of CS/PLA-PIC NPs in HepG2, A549 and MCF7 cells compared to CS-PLA NPs and PIC.
29298541	0	38	theme	cancer	124:129	arg1	lines					136:140	liver, lung and breast cancer cell lines	101:140	liver, lung and breast cancer cell lines	101:140	Chitosan/poly (lactic acid)-coated piceatannol nanoparticles exert an in vitro apoptosis activity on liver, lung and breast cancer cell lines.
29298541	6	39	theme	LDH	715:717	arg1	assays					719:724	MTT and LDH assays	707:724	MTT and LDH assays	707:724	MTT and LDH assays revealed higher cytotoxic efficacy of CS/PLA-PIC NPs in HepG2, A549 and MCF7 cells compared to CS-PLA NPs and PIC.
29298541	4	40	dep	in	543:544	arg1	vitro					546:550	vitro	546:550	vitro	546:550	The in vitro drug release study showed that NPs provided a continuous release of the entrapped PIC.
29298541	8	41	theme	CS/PLA-PIC	957:966	arg1	NPs					968:970	CS/PLA-PIC NPs	957:970	CS/PLA-PIC NPs	957:970	Furthermore, cells treated with CS/PLA-PIC NPs showed fragmenting DNA and also demonstrated for apoptotic cells percentage by flow cytometry.
29298541	4	42	theme	PIC	634:636	arg1	release					609:615	a continuous release	596:615	a continuous release of the entrapped PIC	596:636	The in vitro drug release study showed that NPs provided a continuous release of the entrapped PIC.
29298541	7	43	theme	apoptotic	895:903	arg1	cells					918:922	apoptotic and necrotic cells	895:922	apoptotic and necrotic cells	895:922	Dual staining results showed the early/late-stages of apoptotic and necrotic cells.
29298541	6	44	theme	MTT	707:709	arg1	assays					719:724	MTT and LDH assays	707:724	MTT and LDH assays	707:724	MTT and LDH assays revealed higher cytotoxic efficacy of CS/PLA-PIC NPs in HepG2, A549 and MCF7 cells compared to CS-PLA NPs and PIC.
29298541	8	45	theme	flow	1051:1054	arg1	cytometry					1056:1064	flow cytometry	1051:1064	flow cytometry	1051:1064	Furthermore, cells treated with CS/PLA-PIC NPs showed fragmenting DNA and also demonstrated for apoptotic cells percentage by flow cytometry.
29298541	7	46	theme	Dual	841:844	arg1	results					855:861	Dual staining results	841:861	Dual staining results	841:861	Dual staining results showed the early/late-stages of apoptotic and necrotic cells.
29298541	4	47	theme	entrapped	624:632	arg1	PIC					634:636	the entrapped PIC	620:636	the entrapped PIC	620:636	The in vitro drug release study showed that NPs provided a continuous release of the entrapped PIC.
29298541	9	48	theme	cancer	1146:1151	arg1	viability					1158:1166	cancer cell viability	1146:1166	cancer cell viability	1146:1166	These results suggested that upon CS/PLA-PIC NPs exposure leads to decrease in cancer cell viability due to apoptosis.
29298541	3	49	theme	encapsulation	511:523	arg1	efficiencies					525:536	encapsulation efficiencies	511:536	encapsulation efficiencies	511:536	The preliminary and stability studies were determined for the polymers drug-loading capacity and encapsulation efficiencies.
29298541	1	50	theme	study	158:162	arg1	aim					147:149	The aim	143:149	The aim of the study	143:162	The aim of the study was to synthesize nanoparticles (NPs) with chitosan (CS), and poly (lactic acid) (PLA) as a carrier for the drug piceatannol (PIC).
29298541	9	51	theme	cell	1153:1156	arg1	viability					1158:1166	cancer cell viability	1146:1166	cancer cell viability	1146:1166	These results suggested that upon CS/PLA-PIC NPs exposure leads to decrease in cancer cell viability due to apoptosis.
29298541	7	52	theme	cells	918:922	arg1	early/late-stages					874:890	the early/late-stages	870:890	the early/late-stages of apoptotic and necrotic cells	870:922	Dual staining results showed the early/late-stages of apoptotic and necrotic cells.
29298541	1	53	with	nanoparticles	182:194	arg1	chitosan					207:214	chitosan	207:214	chitosan (CS)	207:219	The aim of the study was to synthesize nanoparticles (NPs) with chitosan (CS), and poly (lactic acid) (PLA) as a carrier for the drug piceatannol (PIC).
29298541	1	53	with	nanoparticles	182:194	arg1	poly					226:229	poly	226:229	poly (lactic acid) (PLA)	226:249	The aim of the study was to synthesize nanoparticles (NPs) with chitosan (CS), and poly (lactic acid) (PLA) as a carrier for the drug piceatannol (PIC).
29298541	1	53	with	nanoparticles	182:194	arg1	acid					239:242	lactic acid	232:242	lactic acid	232:242	The aim of the study was to synthesize nanoparticles (NPs) with chitosan (CS), and poly (lactic acid) (PLA) as a carrier for the drug piceatannol (PIC).
29298541	1	53	with	nanoparticles	182:194	arg1	PLA					246:248	PLA	246:248	PLA	246:248	The aim of the study was to synthesize nanoparticles (NPs) with chitosan (CS), and poly (lactic acid) (PLA) as a carrier for the drug piceatannol (PIC).
29298541	1	53	with	nanoparticles	182:194	arg1	CS					217:218	CS	217:218	CS	217:218	The aim of the study was to synthesize nanoparticles (NPs) with chitosan (CS), and poly (lactic acid) (PLA) as a carrier for the drug piceatannol (PIC).
29298541	6	54	from	efficacy	752:759	arg1	cells					803:807	MCF7 cells	798:807	MCF7 cells	798:807	MTT and LDH assays revealed higher cytotoxic efficacy of CS/PLA-PIC NPs in HepG2, A549 and MCF7 cells compared to CS-PLA NPs and PIC.
29298541	6	54	from	efficacy	752:759	arg1	HepG2					782:786	HepG2	782:786	HepG2	782:786	MTT and LDH assays revealed higher cytotoxic efficacy of CS/PLA-PIC NPs in HepG2, A549 and MCF7 cells compared to CS-PLA NPs and PIC.
29298541	6	54	from	efficacy	752:759	arg1	A549					789:792	A549	789:792	A549	789:792	MTT and LDH assays revealed higher cytotoxic efficacy of CS/PLA-PIC NPs in HepG2, A549 and MCF7 cells compared to CS-PLA NPs and PIC.
30165149	5	0	theme	/Alg	759:762	arg1	beads					764:768	sericin-derived activated carbon (S-AC)/Alg beads	720:768	sericin-derived activated carbon (S-AC)/Alg beads	720:768	After that, methylene blue (MB) removal behavior of sericin-derived activated carbon (S-AC)/Alg beads was investigated.
30165149	7	1	theme	process	1193:1199	arg1	5 cycles					1146:1153	5 cycles	1146:1153	5 cycles of the repeated adsorption-desorption process	1146:1199	S-AC/Alg beads had an excellent MB adsorption capacity (502.5 mg/g) with stable regeneration stability, and 90.1% of the original removal efficiency was maintained after 5 cycles of the repeated adsorption-desorption process.
30165149	8	2	theme	contaminants	1308:1319	arg1	removal					1297:1303	the removal	1293:1303	the removal of contaminants in aqueous solutions	1293:1340	These findings reveal that S-AC/Alg composite beads can be used as low-cost adsorbents for the removal of contaminants in aqueous solutions.
30165149	7	3	theme	adsorption	1011:1020	arg1	502.5 mg/g					1032:1041	502.5 mg/g	1032:1041	502.5 mg/g	1032:1041	S-AC/Alg beads had an excellent MB adsorption capacity (502.5 mg/g) with stable regeneration stability, and 90.1% of the original removal efficiency was maintained after 5 cycles of the repeated adsorption-desorption process.
30165149	7	3	theme	adsorption	1011:1020	arg1	capacity					1022:1029	an excellent MB adsorption capacity	995:1029	an excellent MB adsorption capacity (502.5 mg/g)	995:1042	S-AC/Alg beads had an excellent MB adsorption capacity (502.5 mg/g) with stable regeneration stability, and 90.1% of the original removal efficiency was maintained after 5 cycles of the repeated adsorption-desorption process.
30165149	0	4	theme	natural	83:89	arg1	polymer-based					91:103	An effective and recyclable natural polymer-based	55:103	Sericin-derived activated carbon-loaded alginate bead: An effective and recyclable natural polymer-based adsorbent for methylene blue removal.	0:141	Sericin-derived activated carbon-loaded alginate bead: An effective and recyclable natural polymer-based adsorbent for methylene blue removal.
30165149	6	5	theme	porous	884:889	arg1	structure					891:899	this porous structure	879:899	this porous structure of S-AC	879:907	S-AC obtained by NaOH chemical activation had a larger BET surface area (2150.1 m2/g), and this porous structure of S-AC was well maintained after S-AC/Alg bead preparation (1215.4 m2/g).
30165149	6	6	theme	chemical	810:817	arg1	activation					819:828	NaOH chemical activation	805:828	NaOH chemical activation	805:828	S-AC obtained by NaOH chemical activation had a larger BET surface area (2150.1 m2/g), and this porous structure of S-AC was well maintained after S-AC/Alg bead preparation (1215.4 m2/g).
30165149	2	7	theme	virtuous	381:388	arg1	cycle					390:394	the virtuous cycle	377:394	the virtuous cycle of natural polymers	377:414	Preparation of activated carbon using agricultural by-products is environmentally friendly and can greatly contribute to the virtuous cycle of natural polymers.
30165149	5	8	theme	sericin-derived	720:734	arg1	beads					764:768	sericin-derived activated carbon (S-AC)/Alg beads	720:768	sericin-derived activated carbon (S-AC)/Alg beads	720:768	After that, methylene blue (MB) removal behavior of sericin-derived activated carbon (S-AC)/Alg beads was investigated.
30165149	8	9	theme	aqueous	1324:1330	arg1	solutions					1332:1340	aqueous solutions	1324:1340	aqueous solutions	1324:1340	These findings reveal that S-AC/Alg composite beads can be used as low-cost adsorbents for the removal of contaminants in aqueous solutions.
30165149	6	10	theme	NaOH	805:808	arg1	activation					819:828	NaOH chemical activation	805:828	NaOH chemical activation	805:828	S-AC obtained by NaOH chemical activation had a larger BET surface area (2150.1 m2/g), and this porous structure of S-AC was well maintained after S-AC/Alg bead preparation (1215.4 m2/g).
30165149	1	11	used	used	176:179	arg2	carbon					153:158	Activated carbon	143:158	Activated carbon	143:158	Activated carbon has been widely used as an effective adsorbent for removing contaminants from the water stream.
30165149	1	11	used	used	176:179	arg2	effective					187:195	effective	187:195	effective	187:195	Activated carbon has been widely used as an effective adsorbent for removing contaminants from the water stream.
30165149	0	12	theme	adsorbent	105:113	arg1	polymer-based					91:103	An effective and recyclable natural polymer-based	55:103	Sericin-derived activated carbon-loaded alginate bead: An effective and recyclable natural polymer-based adsorbent for methylene blue removal.	0:141	Sericin-derived activated carbon-loaded alginate bead: An effective and recyclable natural polymer-based adsorbent for methylene blue removal.
30165149	5	13	theme	removal	700:706	arg1	behavior					708:715	methylene blue (MB) removal behavior	680:715	methylene blue (MB) removal behavior of sericin-derived activated carbon (S-AC)/Alg beads	680:768	After that, methylene blue (MB) removal behavior of sericin-derived activated carbon (S-AC)/Alg beads was investigated.
30165149	0	14	theme	blue	129:132	arg1	removal					134:140	methylene blue removal	119:140	methylene blue removal	119:140	Sericin-derived activated carbon-loaded alginate bead: An effective and recyclable natural polymer-based adsorbent for methylene blue removal.
30165149	0	15	link	Sericin-derived	0:14	arg1	bead					49:52	Sericin-derived activated carbon-loaded alginate bead	0:52	Sericin-derived activated carbon-loaded alginate bead: An effective and recyclable natural polymer-based adsorbent for methylene blue removal.	0:141	Sericin-derived activated carbon-loaded alginate bead: An effective and recyclable natural polymer-based adsorbent for methylene blue removal.
30165149	2	16	theme	agricultural	294:305	arg1	by-products					307:317	agricultural by-products	294:317	agricultural by-products	294:317	Preparation of activated carbon using agricultural by-products is environmentally friendly and can greatly contribute to the virtuous cycle of natural polymers.
30165149	8	17	theme	composite	1238:1246	arg1	adsorbents					1278:1287	low-cost adsorbents	1269:1287	low-cost adsorbents	1269:1287	These findings reveal that S-AC/Alg composite beads can be used as low-cost adsorbents for the removal of contaminants in aqueous solutions.
30165149	8	17	theme	composite	1238:1246	arg1	beads					1248:1252	S-AC/Alg composite beads	1229:1252	S-AC/Alg composite beads	1229:1252	These findings reveal that S-AC/Alg composite beads can be used as low-cost adsorbents for the removal of contaminants in aqueous solutions.
30165149	5	18	dep	blue	690:693	arg1	MB					696:697	MB	696:697	MB	696:697	After that, methylene blue (MB) removal behavior of sericin-derived activated carbon (S-AC)/Alg beads was investigated.
30165149	6	19	theme	bead	944:947	arg1	1215.4 m2/g					962:972	1215.4 m2/g	962:972	1215.4 m2/g	962:972	S-AC obtained by NaOH chemical activation had a larger BET surface area (2150.1 m2/g), and this porous structure of S-AC was well maintained after S-AC/Alg bead preparation (1215.4 m2/g).
30165149	6	19	theme	bead	944:947	arg1	preparation					949:959	S-AC/Alg bead preparation	935:959	S-AC/Alg bead preparation (1215.4 m2/g)	935:973	S-AC obtained by NaOH chemical activation had a larger BET surface area (2150.1 m2/g), and this porous structure of S-AC was well maintained after S-AC/Alg bead preparation (1215.4 m2/g).
30165149	8	20	from	contaminants	1308:1319	arg1	solutions					1332:1340	aqueous solutions	1324:1340	aqueous solutions	1324:1340	These findings reveal that S-AC/Alg composite beads can be used as low-cost adsorbents for the removal of contaminants in aqueous solutions.
30165149	7	21	theme	regeneration	1056:1067	arg1	stability					1069:1077	stable regeneration stability	1049:1077	stable regeneration stability	1049:1077	S-AC/Alg beads had an excellent MB adsorption capacity (502.5 mg/g) with stable regeneration stability, and 90.1% of the original removal efficiency was maintained after 5 cycles of the repeated adsorption-desorption process.
30165149	5	22	theme	carbon	746:751	arg1	beads					764:768	sericin-derived activated carbon (S-AC)/Alg beads	720:768	sericin-derived activated carbon (S-AC)/Alg beads	720:768	After that, methylene blue (MB) removal behavior of sericin-derived activated carbon (S-AC)/Alg beads was investigated.
30165149	0	23	theme	activated	16:24	arg1	bead					49:52	Sericin-derived activated carbon-loaded alginate bead	0:52	Sericin-derived activated carbon-loaded alginate bead: An effective and recyclable natural polymer-based adsorbent for methylene blue removal.	0:141	Sericin-derived activated carbon-loaded alginate bead: An effective and recyclable natural polymer-based adsorbent for methylene blue removal.
30165149	5	24	theme	beads	764:768	arg1	behavior					708:715	methylene blue (MB) removal behavior	680:715	methylene blue (MB) removal behavior of sericin-derived activated carbon (S-AC)/Alg beads	680:768	After that, methylene blue (MB) removal behavior of sericin-derived activated carbon (S-AC)/Alg beads was investigated.
30165149	7	25	theme	stable	1049:1054	arg1	stability					1069:1077	stable regeneration stability	1049:1077	stable regeneration stability	1049:1077	S-AC/Alg beads had an excellent MB adsorption capacity (502.5 mg/g) with stable regeneration stability, and 90.1% of the original removal efficiency was maintained after 5 cycles of the repeated adsorption-desorption process.
30165149	5	26	theme	S-AC	754:757	arg1	beads					764:768	sericin-derived activated carbon (S-AC)/Alg beads	720:768	sericin-derived activated carbon (S-AC)/Alg beads	720:768	After that, methylene blue (MB) removal behavior of sericin-derived activated carbon (S-AC)/Alg beads was investigated.
30165149	3	27	theme	activated	446:454	arg1	carbon					456:461	highly porous activated carbon	432:461	highly porous activated carbon	432:461	In this study, highly porous activated carbon was prepared using silk sericin, a secondary protein of the sericulture industry.
30165149	0	28	theme	Sericin-derived	0:14	arg1	bead					49:52	Sericin-derived activated carbon-loaded alginate bead	0:52	Sericin-derived activated carbon-loaded alginate bead: An effective and recyclable natural polymer-based adsorbent for methylene blue removal.	0:141	Sericin-derived activated carbon-loaded alginate bead: An effective and recyclable natural polymer-based adsorbent for methylene blue removal.
30165149	7	29	contain	had	991:993	arg1	beads					985:989	S-AC/Alg beads	976:989	S-AC/Alg beads	976:989	S-AC/Alg beads had an excellent MB adsorption capacity (502.5 mg/g) with stable regeneration stability, and 90.1% of the original removal efficiency was maintained after 5 cycles of the repeated adsorption-desorption process.
30165149	7	29	contain	had	991:993	arg2	502.5 mg/g					1032:1041	502.5 mg/g	1032:1041	502.5 mg/g	1032:1041	S-AC/Alg beads had an excellent MB adsorption capacity (502.5 mg/g) with stable regeneration stability, and 90.1% of the original removal efficiency was maintained after 5 cycles of the repeated adsorption-desorption process.
30165149	7	29	contain	had	991:993	arg2	capacity					1022:1029	an excellent MB adsorption capacity	995:1029	an excellent MB adsorption capacity (502.5 mg/g)	995:1042	S-AC/Alg beads had an excellent MB adsorption capacity (502.5 mg/g) with stable regeneration stability, and 90.1% of the original removal efficiency was maintained after 5 cycles of the repeated adsorption-desorption process.
30165149	8	30	from	removal	1297:1303	arg1	solutions					1332:1340	aqueous solutions	1324:1340	aqueous solutions	1324:1340	These findings reveal that S-AC/Alg composite beads can be used as low-cost adsorbents for the removal of contaminants in aqueous solutions.
30165149	8	31	used	used	1261:1264	arg2	beads					1248:1252	S-AC/Alg composite beads	1229:1252	S-AC/Alg composite beads	1229:1252	These findings reveal that S-AC/Alg composite beads can be used as low-cost adsorbents for the removal of contaminants in aqueous solutions.
30165149	8	31	used	used	1261:1264	arg2	adsorbents					1278:1287	low-cost adsorbents	1269:1287	low-cost adsorbents	1269:1287	These findings reveal that S-AC/Alg composite beads can be used as low-cost adsorbents for the removal of contaminants in aqueous solutions.
30165149	2	32	theme	polymers	407:414	arg1	cycle					390:394	the virtuous cycle	377:394	the virtuous cycle of natural polymers	377:414	Preparation of activated carbon using agricultural by-products is environmentally friendly and can greatly contribute to the virtuous cycle of natural polymers.
30165149	0	33	theme	alginate	40:47	arg1	bead					49:52	Sericin-derived activated carbon-loaded alginate bead	0:52	Sericin-derived activated carbon-loaded alginate bead: An effective and recyclable natural polymer-based adsorbent for methylene blue removal.	0:141	Sericin-derived activated carbon-loaded alginate bead: An effective and recyclable natural polymer-based adsorbent for methylene blue removal.
30165149	4	34	theme	regeneration	573:584	arg1	stability					586:594	regeneration stability	573:594	regeneration stability	573:594	For easy processability and regeneration stability, the bead-type adsorbent was prepared using alginate (Alg) as a matrix.
30165149	0	35	theme	carbon-loaded	26:38	arg1	bead					49:52	Sericin-derived activated carbon-loaded alginate bead	0:52	Sericin-derived activated carbon-loaded alginate bead: An effective and recyclable natural polymer-based adsorbent for methylene blue removal.	0:141	Sericin-derived activated carbon-loaded alginate bead: An effective and recyclable natural polymer-based adsorbent for methylene blue removal.
30165149	6	36	theme	S-AC/Alg	935:942	arg1	1215.4 m2/g					962:972	1215.4 m2/g	962:972	1215.4 m2/g	962:972	S-AC obtained by NaOH chemical activation had a larger BET surface area (2150.1 m2/g), and this porous structure of S-AC was well maintained after S-AC/Alg bead preparation (1215.4 m2/g).
30165149	6	36	theme	S-AC/Alg	935:942	arg1	preparation					949:959	S-AC/Alg bead preparation	935:959	S-AC/Alg bead preparation (1215.4 m2/g)	935:973	S-AC obtained by NaOH chemical activation had a larger BET surface area (2150.1 m2/g), and this porous structure of S-AC was well maintained after S-AC/Alg bead preparation (1215.4 m2/g).
30165149	5	37	link	sericin-derived	720:734	arg1	beads					764:768	sericin-derived activated carbon (S-AC)/Alg beads	720:768	sericin-derived activated carbon (S-AC)/Alg beads	720:768	After that, methylene blue (MB) removal behavior of sericin-derived activated carbon (S-AC)/Alg beads was investigated.
30165149	3	38	theme	sericulture	523:533	arg1	industry					535:542	the sericulture industry	519:542	the sericulture industry	519:542	In this study, highly porous activated carbon was prepared using silk sericin, a secondary protein of the sericulture industry.
30165149	3	39	theme	industry	535:542	arg1	protein					508:514	a secondary protein	496:514	a secondary protein of the sericulture industry	496:542	In this study, highly porous activated carbon was prepared using silk sericin, a secondary protein of the sericulture industry.
30165149	3	39	theme	industry	535:542	arg1	sericin					487:493	silk sericin	482:493	silk sericin	482:493	In this study, highly porous activated carbon was prepared using silk sericin, a secondary protein of the sericulture industry.
30165149	7	40	theme	removal	1106:1112	arg1	efficiency					1114:1123	the original removal efficiency	1093:1123	the original removal efficiency	1093:1123	S-AC/Alg beads had an excellent MB adsorption capacity (502.5 mg/g) with stable regeneration stability, and 90.1% of the original removal efficiency was maintained after 5 cycles of the repeated adsorption-desorption process.
30165149	8	41	theme	S-AC/Alg	1229:1236	arg1	adsorbents					1278:1287	low-cost adsorbents	1269:1287	low-cost adsorbents	1269:1287	These findings reveal that S-AC/Alg composite beads can be used as low-cost adsorbents for the removal of contaminants in aqueous solutions.
30165149	8	41	theme	S-AC/Alg	1229:1236	arg1	beads					1248:1252	S-AC/Alg composite beads	1229:1252	S-AC/Alg composite beads	1229:1252	These findings reveal that S-AC/Alg composite beads can be used as low-cost adsorbents for the removal of contaminants in aqueous solutions.
30165149	3	42	theme	porous	439:444	arg1	carbon					456:461	highly porous activated carbon	432:461	highly porous activated carbon	432:461	In this study, highly porous activated carbon was prepared using silk sericin, a secondary protein of the sericulture industry.
30165149	4	43	theme	easy	549:552	arg1	processability					554:567	easy processability	549:567	easy processability	549:567	For easy processability and regeneration stability, the bead-type adsorbent was prepared using alginate (Alg) as a matrix.
30165149	7	44	theme	efficiency	1114:1123	arg1	efficiency					1114:1123	the original removal efficiency	1093:1123	the original removal efficiency	1093:1123	S-AC/Alg beads had an excellent MB adsorption capacity (502.5 mg/g) with stable regeneration stability, and 90.1% of the original removal efficiency was maintained after 5 cycles of the repeated adsorption-desorption process.
30165149	7	44	theme	efficiency	1114:1123	arg1	%					1088:1088	90.1%	1084:1088	90.1% of the original removal efficiency	1084:1123	S-AC/Alg beads had an excellent MB adsorption capacity (502.5 mg/g) with stable regeneration stability, and 90.1% of the original removal efficiency was maintained after 5 cycles of the repeated adsorption-desorption process.
30165149	6	45	theme	surface	847:853	arg1	2150.1 m2/g					861:871	2150.1 m2/g	861:871	2150.1 m2/g	861:871	S-AC obtained by NaOH chemical activation had a larger BET surface area (2150.1 m2/g), and this porous structure of S-AC was well maintained after S-AC/Alg bead preparation (1215.4 m2/g).
30165149	6	45	theme	surface	847:853	arg1	area					855:858	a larger BET surface area	834:858	a larger BET surface area (2150.1 m2/g)	834:872	S-AC obtained by NaOH chemical activation had a larger BET surface area (2150.1 m2/g), and this porous structure of S-AC was well maintained after S-AC/Alg bead preparation (1215.4 m2/g).
30165149	3	46	theme	silk	482:485	arg1	protein					508:514	a secondary protein	496:514	a secondary protein of the sericulture industry	496:542	In this study, highly porous activated carbon was prepared using silk sericin, a secondary protein of the sericulture industry.
30165149	3	46	theme	silk	482:485	arg1	sericin					487:493	silk sericin	482:493	silk sericin	482:493	In this study, highly porous activated carbon was prepared using silk sericin, a secondary protein of the sericulture industry.
30165149	0	47	theme	effective	58:66	arg1	polymer-based					91:103	An effective and recyclable natural polymer-based	55:103	Sericin-derived activated carbon-loaded alginate bead: An effective and recyclable natural polymer-based adsorbent for methylene blue removal.	0:141	Sericin-derived activated carbon-loaded alginate bead: An effective and recyclable natural polymer-based adsorbent for methylene blue removal.
30165149	7	48	theme	repeated	1162:1169	arg1	process					1193:1199	the repeated adsorption-desorption process	1158:1199	the repeated adsorption-desorption process	1158:1199	S-AC/Alg beads had an excellent MB adsorption capacity (502.5 mg/g) with stable regeneration stability, and 90.1% of the original removal efficiency was maintained after 5 cycles of the repeated adsorption-desorption process.
30165149	1	49	from	stream	248:253	arg1	contaminants					220:231	contaminants	220:231	contaminants from the water stream	220:253	Activated carbon has been widely used as an effective adsorbent for removing contaminants from the water stream.
30165149	6	50	theme	BET	843:845	arg1	2150.1 m2/g					861:871	2150.1 m2/g	861:871	2150.1 m2/g	861:871	S-AC obtained by NaOH chemical activation had a larger BET surface area (2150.1 m2/g), and this porous structure of S-AC was well maintained after S-AC/Alg bead preparation (1215.4 m2/g).
30165149	6	50	theme	BET	843:845	arg1	area					855:858	a larger BET surface area	834:858	a larger BET surface area (2150.1 m2/g)	834:872	S-AC obtained by NaOH chemical activation had a larger BET surface area (2150.1 m2/g), and this porous structure of S-AC was well maintained after S-AC/Alg bead preparation (1215.4 m2/g).
30165149	7	51	theme	S-AC/Alg	976:983	arg1	beads					985:989	S-AC/Alg beads	976:989	S-AC/Alg beads	976:989	S-AC/Alg beads had an excellent MB adsorption capacity (502.5 mg/g) with stable regeneration stability, and 90.1% of the original removal efficiency was maintained after 5 cycles of the repeated adsorption-desorption process.
30165149	8	52	theme	low-cost	1269:1276	arg1	adsorbents					1278:1287	low-cost adsorbents	1269:1287	low-cost adsorbents	1269:1287	These findings reveal that S-AC/Alg composite beads can be used as low-cost adsorbents for the removal of contaminants in aqueous solutions.
30165149	8	52	theme	low-cost	1269:1276	arg1	beads					1248:1252	S-AC/Alg composite beads	1229:1252	S-AC/Alg composite beads	1229:1252	These findings reveal that S-AC/Alg composite beads can be used as low-cost adsorbents for the removal of contaminants in aqueous solutions.
30165149	7	53	theme	original	1097:1104	arg1	efficiency					1114:1123	the original removal efficiency	1093:1123	the original removal efficiency	1093:1123	S-AC/Alg beads had an excellent MB adsorption capacity (502.5 mg/g) with stable regeneration stability, and 90.1% of the original removal efficiency was maintained after 5 cycles of the repeated adsorption-desorption process.
30165149	5	54	theme	blue	690:693	arg1	behavior					708:715	methylene blue (MB) removal behavior	680:715	methylene blue (MB) removal behavior of sericin-derived activated carbon (S-AC)/Alg beads	680:768	After that, methylene blue (MB) removal behavior of sericin-derived activated carbon (S-AC)/Alg beads was investigated.
30165149	6	55	theme	larger	836:841	arg1	2150.1 m2/g					861:871	2150.1 m2/g	861:871	2150.1 m2/g	861:871	S-AC obtained by NaOH chemical activation had a larger BET surface area (2150.1 m2/g), and this porous structure of S-AC was well maintained after S-AC/Alg bead preparation (1215.4 m2/g).
30165149	6	55	theme	larger	836:841	arg1	area					855:858	a larger BET surface area	834:858	a larger BET surface area (2150.1 m2/g)	834:872	S-AC obtained by NaOH chemical activation had a larger BET surface area (2150.1 m2/g), and this porous structure of S-AC was well maintained after S-AC/Alg bead preparation (1215.4 m2/g).
30165149	0	56	dep	bead	49:52	arg1	polymer-based					91:103	An effective and recyclable natural polymer-based	55:103	Sericin-derived activated carbon-loaded alginate bead: An effective and recyclable natural polymer-based adsorbent for methylene blue removal.	0:141	Sericin-derived activated carbon-loaded alginate bead: An effective and recyclable natural polymer-based adsorbent for methylene blue removal.
30165149	7	57	theme	adsorption-desorption	1171:1191	arg1	process					1193:1199	the repeated adsorption-desorption process	1158:1199	the repeated adsorption-desorption process	1158:1199	S-AC/Alg beads had an excellent MB adsorption capacity (502.5 mg/g) with stable regeneration stability, and 90.1% of the original removal efficiency was maintained after 5 cycles of the repeated adsorption-desorption process.
30165149	6	58	theme	S-AC	904:907	arg1	structure					891:899	this porous structure	879:899	this porous structure of S-AC	879:907	S-AC obtained by NaOH chemical activation had a larger BET surface area (2150.1 m2/g), and this porous structure of S-AC was well maintained after S-AC/Alg bead preparation (1215.4 m2/g).
30165149	7	59	theme	excellent	998:1006	arg1	502.5 mg/g					1032:1041	502.5 mg/g	1032:1041	502.5 mg/g	1032:1041	S-AC/Alg beads had an excellent MB adsorption capacity (502.5 mg/g) with stable regeneration stability, and 90.1% of the original removal efficiency was maintained after 5 cycles of the repeated adsorption-desorption process.
30165149	7	59	theme	excellent	998:1006	arg1	capacity					1022:1029	an excellent MB adsorption capacity	995:1029	an excellent MB adsorption capacity (502.5 mg/g)	995:1042	S-AC/Alg beads had an excellent MB adsorption capacity (502.5 mg/g) with stable regeneration stability, and 90.1% of the original removal efficiency was maintained after 5 cycles of the repeated adsorption-desorption process.
30165149	2	60	theme	carbon	281:286	arg1	Preparation					256:266	Preparation	256:266	Preparation of activated carbon using agricultural by-products	256:317	Preparation of activated carbon using agricultural by-products is environmentally friendly and can greatly contribute to the virtuous cycle of natural polymers.
30165149	1	61	theme	Activated	143:151	arg1	effective					187:195	effective	187:195	effective	187:195	Activated carbon has been widely used as an effective adsorbent for removing contaminants from the water stream.
30165149	1	61	theme	Activated	143:151	arg1	carbon					153:158	Activated carbon	143:158	Activated carbon	143:158	Activated carbon has been widely used as an effective adsorbent for removing contaminants from the water stream.
30165149	8	62	from	solutions	1332:1340	arg1	removal					1297:1303	the removal	1293:1303	the removal of contaminants in aqueous solutions	1293:1340	These findings reveal that S-AC/Alg composite beads can be used as low-cost adsorbents for the removal of contaminants in aqueous solutions.
30165149	7	63	theme	MB	1008:1009	arg1	502.5 mg/g					1032:1041	502.5 mg/g	1032:1041	502.5 mg/g	1032:1041	S-AC/Alg beads had an excellent MB adsorption capacity (502.5 mg/g) with stable regeneration stability, and 90.1% of the original removal efficiency was maintained after 5 cycles of the repeated adsorption-desorption process.
30165149	7	63	theme	MB	1008:1009	arg1	capacity					1022:1029	an excellent MB adsorption capacity	995:1029	an excellent MB adsorption capacity (502.5 mg/g)	995:1042	S-AC/Alg beads had an excellent MB adsorption capacity (502.5 mg/g) with stable regeneration stability, and 90.1% of the original removal efficiency was maintained after 5 cycles of the repeated adsorption-desorption process.
30165149	2	64	theme	activated	271:279	arg1	carbon					281:286	activated carbon	271:286	activated carbon using agricultural by-products	271:317	Preparation of activated carbon using agricultural by-products is environmentally friendly and can greatly contribute to the virtuous cycle of natural polymers.
30165149	3	65	theme	secondary	498:506	arg1	protein					508:514	a secondary protein	496:514	a secondary protein of the sericulture industry	496:542	In this study, highly porous activated carbon was prepared using silk sericin, a secondary protein of the sericulture industry.
30165149	3	65	theme	secondary	498:506	arg1	sericin					487:493	silk sericin	482:493	silk sericin	482:493	In this study, highly porous activated carbon was prepared using silk sericin, a secondary protein of the sericulture industry.
30165149	1	66	theme	water	242:246	arg1	stream					248:253	the water stream	238:253	the water stream	238:253	Activated carbon has been widely used as an effective adsorbent for removing contaminants from the water stream.
30165149	0	67	theme	recyclable	72:81	arg1	polymer-based					91:103	An effective and recyclable natural polymer-based	55:103	Sericin-derived activated carbon-loaded alginate bead: An effective and recyclable natural polymer-based adsorbent for methylene blue removal.	0:141	Sericin-derived activated carbon-loaded alginate bead: An effective and recyclable natural polymer-based adsorbent for methylene blue removal.
30165149	2	68	theme	natural	399:405	arg1	polymers					407:414	natural polymers	399:414	natural polymers	399:414	Preparation of activated carbon using agricultural by-products is environmentally friendly and can greatly contribute to the virtuous cycle of natural polymers.
30165149	6	69	contain	had	830:832	arg2	2150.1 m2/g					861:871	2150.1 m2/g	861:871	2150.1 m2/g	861:871	S-AC obtained by NaOH chemical activation had a larger BET surface area (2150.1 m2/g), and this porous structure of S-AC was well maintained after S-AC/Alg bead preparation (1215.4 m2/g).
30165149	6	69	contain	had	830:832	arg1	S-AC					788:791	S-AC	788:791	S-AC obtained by NaOH chemical activation	788:828	S-AC obtained by NaOH chemical activation had a larger BET surface area (2150.1 m2/g), and this porous structure of S-AC was well maintained after S-AC/Alg bead preparation (1215.4 m2/g).
30165149	6	69	contain	had	830:832	arg2	area					855:858	a larger BET surface area	834:858	a larger BET surface area (2150.1 m2/g)	834:872	S-AC obtained by NaOH chemical activation had a larger BET surface area (2150.1 m2/g), and this porous structure of S-AC was well maintained after S-AC/Alg bead preparation (1215.4 m2/g).
30165149	5	70	theme	activated	736:744	arg1	beads					764:768	sericin-derived activated carbon (S-AC)/Alg beads	720:768	sericin-derived activated carbon (S-AC)/Alg beads	720:768	After that, methylene blue (MB) removal behavior of sericin-derived activated carbon (S-AC)/Alg beads was investigated.
30827547	1	0	theme	udder	221:225	arg1	health					227:232	udder health	221:232	udder health	221:232	The aim of the present study was to assess genetic variation and heritability of a novel indicator of udder health, milk differential somatic cell count (DSCC), which represents the percentage of neutrophils plus lymphocytes in the total somatic cell count (SCC).
30827547	4	1	theme	cows	753:756	arg1	records					736:742	10,709 test-day records	720:742	10,709 test-day records of 5,142 cows in 299 herds	720:769	After editing, the final data set included 10,709 test-day records of 5,142 cows in 299 herds.
30827547	11	2	dep	weight	2041:2046	arg1	receive					2072:2078	receive	2072:2078	should receive in a selection program aimed to reduce udder health problems	2065:2139	However, further research is needed to determine the weight this novel trait should receive in a selection program aimed to reduce udder health problems.
30827547	6	3	theme	types	1019:1023	arg1	proportion					986:995	the proportion	982:995	the proportion of the different cell types in milk	982:1031	Comparing our results with the literature offered compelling evidence of the importance of acquiring information about the proportion of the different cell types in milk to better define the udder health status.
30827547	10	4	theme	background	1860:1869	arg1	understanding					1831:1843	the understanding	1827:1843	the understanding of the genetic background of DSCC	1827:1877	From a genetic point of view, the DSCC was positively weakly associated with milk yield, lactose percentage, and milk urea nitrogen, and negatively associated with pH. Our findings contributed to the understanding of the genetic background of DSCC and are a precursor to the potential use of DSCC in breeding programs to enhance cow resistance to mastitis.
30827547	7	5	theme	cell	1238:1241	arg1	score					1243:1247	traditional somatic cell score	1218:1247	traditional somatic cell score (0.04 ± 0.02)	1218:1261	In addition, our analysis revealed, for the first time, that DSCC is a heritable trait, and heritability (0.08 ± 0.02) was higher than that of traditional somatic cell score (0.04 ± 0.02).
30827547	7	5	theme	cell	1238:1241	arg1	±					1255:1255	0.04 ± 0.02	1250:1260	0.04 ± 0.02	1250:1260	In addition, our analysis revealed, for the first time, that DSCC is a heritable trait, and heritability (0.08 ± 0.02) was higher than that of traditional somatic cell score (0.04 ± 0.02).
30827547	4	6	theme	test-day	727:734	arg1	records					736:742	10,709 test-day records	720:742	10,709 test-day records of 5,142 cows in 299 herds	720:769	After editing, the final data set included 10,709 test-day records of 5,142 cows in 299 herds.
30827547	6	7	theme	different	1004:1012	arg1	types					1019:1023	the different cell types	1000:1023	the different cell types	1000:1023	Comparing our results with the literature offered compelling evidence of the importance of acquiring information about the proportion of the different cell types in milk to better define the udder health status.
30827547	10	8	theme	milk	1708:1711	arg1	yield					1713:1717	milk yield	1708:1717	milk yield	1708:1717	From a genetic point of view, the DSCC was positively weakly associated with milk yield, lactose percentage, and milk urea nitrogen, and negatively associated with pH. Our findings contributed to the understanding of the genetic background of DSCC and are a precursor to the potential use of DSCC in breeding programs to enhance cow resistance to mastitis.
30827547	0	9	theme	preliminary	97:107	arg1	analysis					109:116	A preliminary analysis	95:116	Short communication: Genetic aspects of milk differential somatic cell count in Holstein cows: A preliminary analysis.	0:117	Short communication: Genetic aspects of milk differential somatic cell count in Holstein cows: A preliminary analysis.
30827547	1	10	theme	lymphocytes	332:342	arg1	percentage					301:310	the percentage	297:310	the percentage of neutrophils plus lymphocytes	297:342	The aim of the present study was to assess genetic variation and heritability of a novel indicator of udder health, milk differential somatic cell count (DSCC), which represents the percentage of neutrophils plus lymphocytes in the total somatic cell count (SCC).
30827547	10	11	theme	DSCC	1874:1877	arg1	background					1860:1869	the genetic background	1848:1869	the genetic background of DSCC	1848:1877	From a genetic point of view, the DSCC was positively weakly associated with milk yield, lactose percentage, and milk urea nitrogen, and negatively associated with pH. Our findings contributed to the understanding of the genetic background of DSCC and are a precursor to the potential use of DSCC in breeding programs to enhance cow resistance to mastitis.
30827547	3	12	theme	test-day	535:542	arg1	data					544:547	test-day data	535:547	test-day data	535:547	Besides DSCC, test-day data included milk yield, composition traits (i.e., fat, protein, casein, and lactose percentages), pH, milk urea nitrogen, and SCC.
30827547	6	13	theme	health	1060:1065	arg1	status					1067:1072	the udder health status	1050:1072	the udder health status	1050:1072	Comparing our results with the literature offered compelling evidence of the importance of acquiring information about the proportion of the different cell types in milk to better define the udder health status.
30827547	3	14	theme	urea	653:656	arg1	nitrogen					658:665	milk urea nitrogen	648:665	milk urea nitrogen	648:665	Besides DSCC, test-day data included milk yield, composition traits (i.e., fat, protein, casein, and lactose percentages), pH, milk urea nitrogen, and SCC.
30827547	1	15	theme	somatic	357:363	arg1	SCC					377:379	SCC	377:379	SCC	377:379	The aim of the present study was to assess genetic variation and heritability of a novel indicator of udder health, milk differential somatic cell count (DSCC), which represents the percentage of neutrophils plus lymphocytes in the total somatic cell count (SCC).
30827547	1	15	theme	somatic	357:363	arg1	count					370:374	the total somatic cell count	347:374	the total somatic cell count (SCC)	347:380	The aim of the present study was to assess genetic variation and heritability of a novel indicator of udder health, milk differential somatic cell count (DSCC), which represents the percentage of neutrophils plus lymphocytes in the total somatic cell count (SCC).
30827547	2	16	theme	Holstein	506:513	arg1	cows					515:518	Italian Holstein cows	498:518	Italian Holstein cows	498:518	Furthermore, we estimated genetic and phenotypic correlations of DSCC with other milk traits routinely measured in Italian Holstein cows.
30827547	7	17	theme	heritable	1146:1154	arg1	DSCC					1136:1139	DSCC	1136:1139	DSCC	1136:1139	In addition, our analysis revealed, for the first time, that DSCC is a heritable trait, and heritability (0.08 ± 0.02) was higher than that of traditional somatic cell score (0.04 ± 0.02).
30827547	7	17	theme	heritable	1146:1154	arg1	trait					1156:1160	a heritable trait	1144:1160	a heritable trait	1144:1160	In addition, our analysis revealed, for the first time, that DSCC is a heritable trait, and heritability (0.08 ± 0.02) was higher than that of traditional somatic cell score (0.04 ± 0.02).
30827547	10	18	theme	DSCC	1923:1926	arg1	use					1916:1918	the potential use	1902:1918	the potential use of DSCC in breeding programs	1902:1947	From a genetic point of view, the DSCC was positively weakly associated with milk yield, lactose percentage, and milk urea nitrogen, and negatively associated with pH. Our findings contributed to the understanding of the genetic background of DSCC and are a precursor to the potential use of DSCC in breeding programs to enhance cow resistance to mastitis.
30827547	10	19	theme	urea	1749:1752	arg1	nitrogen					1754:1761	milk urea nitrogen	1744:1761	milk urea nitrogen	1744:1761	From a genetic point of view, the DSCC was positively weakly associated with milk yield, lactose percentage, and milk urea nitrogen, and negatively associated with pH. Our findings contributed to the understanding of the genetic background of DSCC and are a precursor to the potential use of DSCC in breeding programs to enhance cow resistance to mastitis.
30827547	1	20	theme	health	227:232	arg1	indicator					208:216	a novel indicator	200:216	a novel indicator of udder health	200:232	The aim of the present study was to assess genetic variation and heritability of a novel indicator of udder health, milk differential somatic cell count (DSCC), which represents the percentage of neutrophils plus lymphocytes in the total somatic cell count (SCC).
30827547	1	20	theme	health	227:232	arg1	count					266:270	milk differential somatic cell count	235:270	milk differential somatic cell count (DSCC)	235:277	The aim of the present study was to assess genetic variation and heritability of a novel indicator of udder health, milk differential somatic cell count (DSCC), which represents the percentage of neutrophils plus lymphocytes in the total somatic cell count (SCC).
30827547	2	21	theme	phenotypic	421:430	arg1	correlations					432:443	genetic and phenotypic correlations	409:443	genetic and phenotypic correlations of DSCC with other milk traits routinely measured in Italian Holstein cows	409:518	Furthermore, we estimated genetic and phenotypic correlations of DSCC with other milk traits routinely measured in Italian Holstein cows.
30827547	0	22	theme	count	71:75	arg1	aspects					29:35	Genetic aspects	21:35	Short communication: Genetic aspects of milk differential somatic cell count in Holstein cows: A preliminary analysis.	0:117	Short communication: Genetic aspects of milk differential somatic cell count in Holstein cows: A preliminary analysis.
30827547	1	23	theme	present	134:140	arg1	study					142:146	the present study	130:146	the present study	130:146	The aim of the present study was to assess genetic variation and heritability of a novel indicator of udder health, milk differential somatic cell count (DSCC), which represents the percentage of neutrophils plus lymphocytes in the total somatic cell count (SCC).
30827547	2	24	theme	milk	464:467	arg1	traits					469:474	other milk traits	458:474	other milk traits routinely measured in Italian Holstein cows	458:518	Furthermore, we estimated genetic and phenotypic correlations of DSCC with other milk traits routinely measured in Italian Holstein cows.
30827547	0	25	theme	Holstein	80:87	arg1	cows					89:92	Holstein cows	80:92	Holstein cows	80:92	Short communication: Genetic aspects of milk differential somatic cell count in Holstein cows: A preliminary analysis.
30827547	9	26	theme	different	1547:1555	arg1	traits					1557:1562	2 different traits	1545:1562	2 different traits	1545:1562	Single-trait analysis within parity showed that DSCC was less heritable in primiparous than in multiparous cows, whereas bivariate analysis confirmed that DSCC and somatic cell score were 2 different traits, as their genetic and phenotypic correlations differed from unity.
30827547	10	27	theme	breeding	1931:1938	arg1	programs					1940:1947	breeding programs	1931:1947	breeding programs	1931:1947	From a genetic point of view, the DSCC was positively weakly associated with milk yield, lactose percentage, and milk urea nitrogen, and negatively associated with pH. Our findings contributed to the understanding of the genetic background of DSCC and are a precursor to the potential use of DSCC in breeding programs to enhance cow resistance to mastitis.
30827547	1	28	theme	neutrophils	315:325	arg1	percentage					301:310	the percentage	297:310	the percentage of neutrophils plus lymphocytes	297:342	The aim of the present study was to assess genetic variation and heritability of a novel indicator of udder health, milk differential somatic cell count (DSCC), which represents the percentage of neutrophils plus lymphocytes in the total somatic cell count (SCC).
30827547	6	29	theme	compelling	913:922	arg1	evidence					924:931	compelling evidence	913:931	compelling evidence of the importance of acquiring information about the proportion of the different cell types in milk to better define the udder health status	913:1072	Comparing our results with the literature offered compelling evidence of the importance of acquiring information about the proportion of the different cell types in milk to better define the udder health status.
30827547	0	30	from	aspects	29:35	arg1	cows					89:92	Holstein cows	80:92	Holstein cows	80:92	Short communication: Genetic aspects of milk differential somatic cell count in Holstein cows: A preliminary analysis.
30827547	11	31	theme	udder	2119:2123	arg1	problems					2132:2139	udder health problems	2119:2139	udder health problems	2119:2139	However, further research is needed to determine the weight this novel trait should receive in a selection program aimed to reduce udder health problems.
30827547	0	32	theme	Short	0:4	arg1	communication					6:18	Short communication	0:18	Short communication: Genetic aspects of milk differential somatic cell count in Holstein cows: A preliminary analysis.	0:117	Short communication: Genetic aspects of milk differential somatic cell count in Holstein cows: A preliminary analysis.
30827547	1	33	theme	milk	235:238	arg1	indicator					208:216	a novel indicator	200:216	a novel indicator of udder health	200:232	The aim of the present study was to assess genetic variation and heritability of a novel indicator of udder health, milk differential somatic cell count (DSCC), which represents the percentage of neutrophils plus lymphocytes in the total somatic cell count (SCC).
30827547	1	33	theme	milk	235:238	arg1	DSCC					273:276	DSCC	273:276	DSCC	273:276	The aim of the present study was to assess genetic variation and heritability of a novel indicator of udder health, milk differential somatic cell count (DSCC), which represents the percentage of neutrophils plus lymphocytes in the total somatic cell count (SCC).
30827547	1	33	theme	milk	235:238	arg1	count					266:270	milk differential somatic cell count	235:270	milk differential somatic cell count (DSCC)	235:277	The aim of the present study was to assess genetic variation and heritability of a novel indicator of udder health, milk differential somatic cell count (DSCC), which represents the percentage of neutrophils plus lymphocytes in the total somatic cell count (SCC).
30827547	10	34	theme	cow	1960:1962	arg1	resistance					1964:1973	cow resistance	1960:1973	cow resistance to mastitis	1960:1985	From a genetic point of view, the DSCC was positively weakly associated with milk yield, lactose percentage, and milk urea nitrogen, and negatively associated with pH. Our findings contributed to the understanding of the genetic background of DSCC and are a precursor to the potential use of DSCC in breeding programs to enhance cow resistance to mastitis.
30827547	6	35	from	proportion	986:995	arg1	milk					1028:1031	milk	1028:1031	milk	1028:1031	Comparing our results with the literature offered compelling evidence of the importance of acquiring information about the proportion of the different cell types in milk to better define the udder health status.
30827547	1	36	theme	somatic	253:259	arg1	indicator					208:216	a novel indicator	200:216	a novel indicator of udder health	200:232	The aim of the present study was to assess genetic variation and heritability of a novel indicator of udder health, milk differential somatic cell count (DSCC), which represents the percentage of neutrophils plus lymphocytes in the total somatic cell count (SCC).
30827547	1	36	theme	somatic	253:259	arg1	DSCC					273:276	DSCC	273:276	DSCC	273:276	The aim of the present study was to assess genetic variation and heritability of a novel indicator of udder health, milk differential somatic cell count (DSCC), which represents the percentage of neutrophils plus lymphocytes in the total somatic cell count (SCC).
30827547	1	36	theme	somatic	253:259	arg1	count					266:270	milk differential somatic cell count	235:270	milk differential somatic cell count (DSCC)	235:277	The aim of the present study was to assess genetic variation and heritability of a novel indicator of udder health, milk differential somatic cell count (DSCC), which represents the percentage of neutrophils plus lymphocytes in the total somatic cell count (SCC).
30827547	2	37	theme	genetic	409:415	arg1	correlations					432:443	genetic and phenotypic correlations	409:443	genetic and phenotypic correlations of DSCC with other milk traits routinely measured in Italian Holstein cows	409:518	Furthermore, we estimated genetic and phenotypic correlations of DSCC with other milk traits routinely measured in Italian Holstein cows.
30827547	0	38	theme	somatic	58:64	arg1	count					71:75	milk differential somatic cell count	40:75	milk differential somatic cell count	40:75	Short communication: Genetic aspects of milk differential somatic cell count in Holstein cows: A preliminary analysis.
30827547	9	39	theme	Single-trait	1357:1368	arg1	analysis					1370:1377	Single-trait analysis	1357:1377	Single-trait analysis within parity	1357:1391	Single-trait analysis within parity showed that DSCC was less heritable in primiparous than in multiparous cows, whereas bivariate analysis confirmed that DSCC and somatic cell score were 2 different traits, as their genetic and phenotypic correlations differed from unity.
30827547	7	40	theme	first	1119:1123	arg1	time					1125:1128	the first time	1115:1128	the first time	1115:1128	In addition, our analysis revealed, for the first time, that DSCC is a heritable trait, and heritability (0.08 ± 0.02) was higher than that of traditional somatic cell score (0.04 ± 0.02).
30827547	3	41	theme	composition	570:580	arg1	traits					582:587	composition traits	570:587	composition traits (i.e., fat, protein, casein, and lactose percentages)	570:641	Besides DSCC, test-day data included milk yield, composition traits (i.e., fat, protein, casein, and lactose percentages), pH, milk urea nitrogen, and SCC.
30827547	4	42	theme	data	702:705	arg1	set					707:709	the final data set	692:709	the final data set	692:709	After editing, the final data set included 10,709 test-day records of 5,142 cows in 299 herds.
30827547	0	43	theme	milk	40:43	arg1	count					71:75	milk differential somatic cell count	40:75	milk differential somatic cell count	40:75	Short communication: Genetic aspects of milk differential somatic cell count in Holstein cows: A preliminary analysis.
30827547	8	44	theme	quality	1341:1347	arg1	traits					1349:1354	quality traits	1341:1354	quality traits	1341:1354	Nevertheless, heritability of DSCC is still low compared with milk yield and quality traits.
30827547	5	45	theme	SCC	858:860	arg1	%					847:847	approximately 38%	831:847	approximately 38% of total SCC	831:860	Mean of DSCC was 62.07%, which means that macrophages were approximately 38% of total SCC.
30827547	5	45	theme	SCC	858:860	arg1	SCC					858:860	total SCC	852:860	total SCC	852:860	Mean of DSCC was 62.07%, which means that macrophages were approximately 38% of total SCC.
30827547	1	46	theme	novel	202:206	arg1	indicator					208:216	a novel indicator	200:216	a novel indicator of udder health	200:232	The aim of the present study was to assess genetic variation and heritability of a novel indicator of udder health, milk differential somatic cell count (DSCC), which represents the percentage of neutrophils plus lymphocytes in the total somatic cell count (SCC).
30827547	1	46	theme	novel	202:206	arg1	count					266:270	milk differential somatic cell count	235:270	milk differential somatic cell count (DSCC)	235:277	The aim of the present study was to assess genetic variation and heritability of a novel indicator of udder health, milk differential somatic cell count (DSCC), which represents the percentage of neutrophils plus lymphocytes in the total somatic cell count (SCC).
30827547	6	47	theme	importance	940:949	arg1	evidence					924:931	compelling evidence	913:931	compelling evidence of the importance of acquiring information about the proportion of the different cell types in milk to better define the udder health status	913:1072	Comparing our results with the literature offered compelling evidence of the importance of acquiring information about the proportion of the different cell types in milk to better define the udder health status.
30827547	9	48	theme	cell	1529:1532	arg1	score					1534:1538	somatic cell score	1521:1538	somatic cell score	1521:1538	Single-trait analysis within parity showed that DSCC was less heritable in primiparous than in multiparous cows, whereas bivariate analysis confirmed that DSCC and somatic cell score were 2 different traits, as their genetic and phenotypic correlations differed from unity.
30827547	11	49	theme	novel	2053:2057	arg1	trait					2059:2063	this novel trait	2048:2063	this novel trait	2048:2063	However, further research is needed to determine the weight this novel trait should receive in a selection program aimed to reduce udder health problems.
30827547	10	50	theme	view	1655:1658	arg1	point					1646:1650	a genetic point	1636:1650	a genetic point of view	1636:1658	From a genetic point of view, the DSCC was positively weakly associated with milk yield, lactose percentage, and milk urea nitrogen, and negatively associated with pH. Our findings contributed to the understanding of the genetic background of DSCC and are a precursor to the potential use of DSCC in breeding programs to enhance cow resistance to mastitis.
30827547	3	51	dep	fat	596:598	arg1	percentages					630:640	percentages	630:640	percentages	630:640	Besides DSCC, test-day data included milk yield, composition traits (i.e., fat, protein, casein, and lactose percentages), pH, milk urea nitrogen, and SCC.
30827547	3	51	dep	fat	596:598	arg1	i.e.					590:593	i.e.	590:593	i.e.	590:593	Besides DSCC, test-day data included milk yield, composition traits (i.e., fat, protein, casein, and lactose percentages), pH, milk urea nitrogen, and SCC.
30827547	7	52	theme	traditional	1218:1228	arg1	score					1243:1247	traditional somatic cell score	1218:1247	traditional somatic cell score (0.04 ± 0.02)	1218:1261	In addition, our analysis revealed, for the first time, that DSCC is a heritable trait, and heritability (0.08 ± 0.02) was higher than that of traditional somatic cell score (0.04 ± 0.02).
30827547	7	52	theme	traditional	1218:1228	arg1	±					1255:1255	0.04 ± 0.02	1250:1260	0.04 ± 0.02	1250:1260	In addition, our analysis revealed, for the first time, that DSCC is a heritable trait, and heritability (0.08 ± 0.02) was higher than that of traditional somatic cell score (0.04 ± 0.02).
30827547	1	53	theme	study	142:146	arg1	aim					123:125	The aim	119:125	The aim of the present study	119:146	The aim of the present study was to assess genetic variation and heritability of a novel indicator of udder health, milk differential somatic cell count (DSCC), which represents the percentage of neutrophils plus lymphocytes in the total somatic cell count (SCC).
30827547	7	54	theme	somatic	1230:1236	arg1	score					1243:1247	traditional somatic cell score	1218:1247	traditional somatic cell score (0.04 ± 0.02)	1218:1261	In addition, our analysis revealed, for the first time, that DSCC is a heritable trait, and heritability (0.08 ± 0.02) was higher than that of traditional somatic cell score (0.04 ± 0.02).
30827547	7	54	theme	somatic	1230:1236	arg1	±					1255:1255	0.04 ± 0.02	1250:1260	0.04 ± 0.02	1250:1260	In addition, our analysis revealed, for the first time, that DSCC is a heritable trait, and heritability (0.08 ± 0.02) was higher than that of traditional somatic cell score (0.04 ± 0.02).
30827547	6	55	theme	cell	1014:1017	arg1	types					1019:1023	the different cell types	1000:1023	the different cell types	1000:1023	Comparing our results with the literature offered compelling evidence of the importance of acquiring information about the proportion of the different cell types in milk to better define the udder health status.
30827547	10	56	theme	genetic	1852:1858	arg1	background					1860:1869	the genetic background	1848:1869	the genetic background of DSCC	1848:1877	From a genetic point of view, the DSCC was positively weakly associated with milk yield, lactose percentage, and milk urea nitrogen, and negatively associated with pH. Our findings contributed to the understanding of the genetic background of DSCC and are a precursor to the potential use of DSCC in breeding programs to enhance cow resistance to mastitis.
30827547	9	57	theme	multiparous	1452:1462	arg1	cows					1464:1467	multiparous cows	1452:1467	multiparous cows	1452:1467	Single-trait analysis within parity showed that DSCC was less heritable in primiparous than in multiparous cows, whereas bivariate analysis confirmed that DSCC and somatic cell score were 2 different traits, as their genetic and phenotypic correlations differed from unity.
30827547	3	58	theme	milk	648:651	arg1	nitrogen					658:665	milk urea nitrogen	648:665	milk urea nitrogen	648:665	Besides DSCC, test-day data included milk yield, composition traits (i.e., fat, protein, casein, and lactose percentages), pH, milk urea nitrogen, and SCC.
30827547	1	59	theme	genetic	162:168	arg1	variation					170:178	genetic variation	162:178	genetic variation	162:178	The aim of the present study was to assess genetic variation and heritability of a novel indicator of udder health, milk differential somatic cell count (DSCC), which represents the percentage of neutrophils plus lymphocytes in the total somatic cell count (SCC).
30827547	9	60	from	primiparous	1432:1442	arg1	heritable					1419:1427	heritable	1419:1427	heritable	1419:1427	Single-trait analysis within parity showed that DSCC was less heritable in primiparous than in multiparous cows, whereas bivariate analysis confirmed that DSCC and somatic cell score were 2 different traits, as their genetic and phenotypic correlations differed from unity.
30827547	10	61	from	use	1916:1918	arg1	programs					1940:1947	breeding programs	1931:1947	breeding programs	1931:1947	From a genetic point of view, the DSCC was positively weakly associated with milk yield, lactose percentage, and milk urea nitrogen, and negatively associated with pH. Our findings contributed to the understanding of the genetic background of DSCC and are a precursor to the potential use of DSCC in breeding programs to enhance cow resistance to mastitis.
30827547	9	62	from	heritable	1419:1427	arg1	cows					1464:1467	multiparous cows	1452:1467	multiparous cows	1452:1467	Single-trait analysis within parity showed that DSCC was less heritable in primiparous than in multiparous cows, whereas bivariate analysis confirmed that DSCC and somatic cell score were 2 different traits, as their genetic and phenotypic correlations differed from unity.
30827547	9	62	from	heritable	1419:1427	arg1	primiparous					1432:1442	primiparous	1432:1442	primiparous	1432:1442	Single-trait analysis within parity showed that DSCC was less heritable in primiparous than in multiparous cows, whereas bivariate analysis confirmed that DSCC and somatic cell score were 2 different traits, as their genetic and phenotypic correlations differed from unity.
30827547	9	63	theme	bivariate	1478:1486	arg1	analysis					1488:1495	bivariate analysis	1478:1495	bivariate analysis	1478:1495	Single-trait analysis within parity showed that DSCC was less heritable in primiparous than in multiparous cows, whereas bivariate analysis confirmed that DSCC and somatic cell score were 2 different traits, as their genetic and phenotypic correlations differed from unity.
30827547	10	64	theme	potential	1906:1914	arg1	use					1916:1918	the potential use	1902:1918	the potential use of DSCC in breeding programs	1902:1947	From a genetic point of view, the DSCC was positively weakly associated with milk yield, lactose percentage, and milk urea nitrogen, and negatively associated with pH. Our findings contributed to the understanding of the genetic background of DSCC and are a precursor to the potential use of DSCC in breeding programs to enhance cow resistance to mastitis.
30827547	9	65	dep	showed	1393:1398	arg1	whereas					1470:1476	whereas	1470:1476	whereas	1470:1476	Single-trait analysis within parity showed that DSCC was less heritable in primiparous than in multiparous cows, whereas bivariate analysis confirmed that DSCC and somatic cell score were 2 different traits, as their genetic and phenotypic correlations differed from unity.
30827547	11	66	theme	further	1997:2003	arg1	research					2005:2012	further research	1997:2012	further research	1997:2012	However, further research is needed to determine the weight this novel trait should receive in a selection program aimed to reduce udder health problems.
30827547	1	67	theme	total	351:355	arg1	SCC					377:379	SCC	377:379	SCC	377:379	The aim of the present study was to assess genetic variation and heritability of a novel indicator of udder health, milk differential somatic cell count (DSCC), which represents the percentage of neutrophils plus lymphocytes in the total somatic cell count (SCC).
30827547	1	67	theme	total	351:355	arg1	count					370:374	the total somatic cell count	347:374	the total somatic cell count (SCC)	347:380	The aim of the present study was to assess genetic variation and heritability of a novel indicator of udder health, milk differential somatic cell count (DSCC), which represents the percentage of neutrophils plus lymphocytes in the total somatic cell count (SCC).
30827547	6	68	theme	udder	1054:1058	arg1	status					1067:1072	the udder health status	1050:1072	the udder health status	1050:1072	Comparing our results with the literature offered compelling evidence of the importance of acquiring information about the proportion of the different cell types in milk to better define the udder health status.
30827547	11	69	theme	health	2125:2130	arg1	problems					2132:2139	udder health problems	2119:2139	udder health problems	2119:2139	However, further research is needed to determine the weight this novel trait should receive in a selection program aimed to reduce udder health problems.
30827547	10	70	theme	lactose	1720:1726	arg1	percentage					1728:1737	lactose percentage	1720:1737	lactose percentage	1720:1737	From a genetic point of view, the DSCC was positively weakly associated with milk yield, lactose percentage, and milk urea nitrogen, and negatively associated with pH. Our findings contributed to the understanding of the genetic background of DSCC and are a precursor to the potential use of DSCC in breeding programs to enhance cow resistance to mastitis.
30827547	1	71	theme	cell	365:368	arg1	SCC					377:379	SCC	377:379	SCC	377:379	The aim of the present study was to assess genetic variation and heritability of a novel indicator of udder health, milk differential somatic cell count (DSCC), which represents the percentage of neutrophils plus lymphocytes in the total somatic cell count (SCC).
30827547	1	71	theme	cell	365:368	arg1	count					370:374	the total somatic cell count	347:374	the total somatic cell count (SCC)	347:380	The aim of the present study was to assess genetic variation and heritability of a novel indicator of udder health, milk differential somatic cell count (DSCC), which represents the percentage of neutrophils plus lymphocytes in the total somatic cell count (SCC).
30827547	3	72	theme	milk	558:561	arg1	yield					563:567	milk yield	558:567	milk yield	558:567	Besides DSCC, test-day data included milk yield, composition traits (i.e., fat, protein, casein, and lactose percentages), pH, milk urea nitrogen, and SCC.
30827547	5	73	theme	DSCC	780:783	arg1	Mean					772:775	Mean	772:775	Mean of DSCC	772:783	Mean of DSCC was 62.07%, which means that macrophages were approximately 38% of total SCC.
30827547	2	74	theme	Italian	498:504	arg1	cows					515:518	Italian Holstein cows	498:518	Italian Holstein cows	498:518	Furthermore, we estimated genetic and phenotypic correlations of DSCC with other milk traits routinely measured in Italian Holstein cows.
30827547	0	75	theme	cell	66:69	arg1	count					71:75	milk differential somatic cell count	40:75	milk differential somatic cell count	40:75	Short communication: Genetic aspects of milk differential somatic cell count in Holstein cows: A preliminary analysis.
30827547	9	76	theme	somatic	1521:1527	arg1	score					1534:1538	somatic cell score	1521:1538	somatic cell score	1521:1538	Single-trait analysis within parity showed that DSCC was less heritable in primiparous than in multiparous cows, whereas bivariate analysis confirmed that DSCC and somatic cell score were 2 different traits, as their genetic and phenotypic correlations differed from unity.
30827547	10	77	theme	milk	1744:1747	arg1	nitrogen					1754:1761	milk urea nitrogen	1744:1761	milk urea nitrogen	1744:1761	From a genetic point of view, the DSCC was positively weakly associated with milk yield, lactose percentage, and milk urea nitrogen, and negatively associated with pH. Our findings contributed to the understanding of the genetic background of DSCC and are a precursor to the potential use of DSCC in breeding programs to enhance cow resistance to mastitis.
30827547	11	78	theme	selection	2085:2093	arg1	program					2095:2101	a selection program	2083:2101	a selection program aimed to reduce udder health problems	2083:2139	However, further research is needed to determine the weight this novel trait should receive in a selection program aimed to reduce udder health problems.
30827547	4	79	from	records	736:742	arg1	herds					765:769	299 herds	761:769	299 herds	761:769	After editing, the final data set included 10,709 test-day records of 5,142 cows in 299 herds.
30827547	9	80	from	cows	1464:1467	arg1	heritable					1419:1427	heritable	1419:1427	heritable	1419:1427	Single-trait analysis within parity showed that DSCC was less heritable in primiparous than in multiparous cows, whereas bivariate analysis confirmed that DSCC and somatic cell score were 2 different traits, as their genetic and phenotypic correlations differed from unity.
30827547	2	81	theme	other	458:462	arg1	traits					469:474	other milk traits	458:474	other milk traits routinely measured in Italian Holstein cows	458:518	Furthermore, we estimated genetic and phenotypic correlations of DSCC with other milk traits routinely measured in Italian Holstein cows.
30827547	8	82	theme	DSCC	1294:1297	arg1	heritability					1278:1289	heritability	1278:1289	heritability of DSCC	1278:1297	Nevertheless, heritability of DSCC is still low compared with milk yield and quality traits.
30827547	2	83	with	correlations	432:443	arg1	traits					469:474	other milk traits	458:474	other milk traits routinely measured in Italian Holstein cows	458:518	Furthermore, we estimated genetic and phenotypic correlations of DSCC with other milk traits routinely measured in Italian Holstein cows.
30827547	2	84	theme	DSCC	448:451	arg1	correlations					432:443	genetic and phenotypic correlations	409:443	genetic and phenotypic correlations of DSCC with other milk traits routinely measured in Italian Holstein cows	409:518	Furthermore, we estimated genetic and phenotypic correlations of DSCC with other milk traits routinely measured in Italian Holstein cows.
30827547	0	85	theme	Genetic	21:27	arg1	aspects					29:35	Genetic aspects	21:35	Short communication: Genetic aspects of milk differential somatic cell count in Holstein cows: A preliminary analysis.	0:117	Short communication: Genetic aspects of milk differential somatic cell count in Holstein cows: A preliminary analysis.
30827547	1	86	theme	differential	240:251	arg1	indicator					208:216	a novel indicator	200:216	a novel indicator of udder health	200:232	The aim of the present study was to assess genetic variation and heritability of a novel indicator of udder health, milk differential somatic cell count (DSCC), which represents the percentage of neutrophils plus lymphocytes in the total somatic cell count (SCC).
30827547	1	86	theme	differential	240:251	arg1	DSCC					273:276	DSCC	273:276	DSCC	273:276	The aim of the present study was to assess genetic variation and heritability of a novel indicator of udder health, milk differential somatic cell count (DSCC), which represents the percentage of neutrophils plus lymphocytes in the total somatic cell count (SCC).
30827547	1	86	theme	differential	240:251	arg1	count					266:270	milk differential somatic cell count	235:270	milk differential somatic cell count (DSCC)	235:277	The aim of the present study was to assess genetic variation and heritability of a novel indicator of udder health, milk differential somatic cell count (DSCC), which represents the percentage of neutrophils plus lymphocytes in the total somatic cell count (SCC).
30827547	9	87	theme	genetic	1574:1580	arg1	correlations					1597:1608	their genetic and phenotypic correlations	1568:1608	their genetic and phenotypic correlations	1568:1608	Single-trait analysis within parity showed that DSCC was less heritable in primiparous than in multiparous cows, whereas bivariate analysis confirmed that DSCC and somatic cell score were 2 different traits, as their genetic and phenotypic correlations differed from unity.
30827547	8	88	theme	milk	1326:1329	arg1	yield					1331:1335	milk yield	1326:1335	milk yield	1326:1335	Nevertheless, heritability of DSCC is still low compared with milk yield and quality traits.
30827547	1	89	theme	cell	261:264	arg1	indicator					208:216	a novel indicator	200:216	a novel indicator of udder health	200:232	The aim of the present study was to assess genetic variation and heritability of a novel indicator of udder health, milk differential somatic cell count (DSCC), which represents the percentage of neutrophils plus lymphocytes in the total somatic cell count (SCC).
30827547	1	89	theme	cell	261:264	arg1	DSCC					273:276	DSCC	273:276	DSCC	273:276	The aim of the present study was to assess genetic variation and heritability of a novel indicator of udder health, milk differential somatic cell count (DSCC), which represents the percentage of neutrophils plus lymphocytes in the total somatic cell count (SCC).
30827547	1	89	theme	cell	261:264	arg1	count					266:270	milk differential somatic cell count	235:270	milk differential somatic cell count (DSCC)	235:277	The aim of the present study was to assess genetic variation and heritability of a novel indicator of udder health, milk differential somatic cell count (DSCC), which represents the percentage of neutrophils plus lymphocytes in the total somatic cell count (SCC).
30827547	5	90	theme	total	852:856	arg1	SCC					858:860	total SCC	852:860	total SCC	852:860	Mean of DSCC was 62.07%, which means that macrophages were approximately 38% of total SCC.
30827547	9	91	theme	phenotypic	1586:1595	arg1	correlations					1597:1608	their genetic and phenotypic correlations	1568:1608	their genetic and phenotypic correlations	1568:1608	Single-trait analysis within parity showed that DSCC was less heritable in primiparous than in multiparous cows, whereas bivariate analysis confirmed that DSCC and somatic cell score were 2 different traits, as their genetic and phenotypic correlations differed from unity.
30827547	0	92	theme	differential	45:56	arg1	count					71:75	milk differential somatic cell count	40:75	milk differential somatic cell count	40:75	Short communication: Genetic aspects of milk differential somatic cell count in Holstein cows: A preliminary analysis.
30827547	4	93	theme	final	696:700	arg1	set					707:709	the final data set	692:709	the final data set	692:709	After editing, the final data set included 10,709 test-day records of 5,142 cows in 299 herds.
30827547	10	94	theme	genetic	1638:1644	arg1	point					1646:1650	a genetic point	1636:1650	a genetic point of view	1636:1658	From a genetic point of view, the DSCC was positively weakly associated with milk yield, lactose percentage, and milk urea nitrogen, and negatively associated with pH. Our findings contributed to the understanding of the genetic background of DSCC and are a precursor to the potential use of DSCC in breeding programs to enhance cow resistance to mastitis.
30827547	0	95	dep	communication	6:18	arg1	analysis					109:116	A preliminary analysis	95:116	Short communication: Genetic aspects of milk differential somatic cell count in Holstein cows: A preliminary analysis.	0:117	Short communication: Genetic aspects of milk differential somatic cell count in Holstein cows: A preliminary analysis.
30827547	0	95	dep	communication	6:18	arg1	aspects					29:35	Genetic aspects	21:35	Short communication: Genetic aspects of milk differential somatic cell count in Holstein cows: A preliminary analysis.	0:117	Short communication: Genetic aspects of milk differential somatic cell count in Holstein cows: A preliminary analysis.
30827547	10	96	theme	pH.	1795:1797	arg1	findings					1803:1810	pH. Our findings	1795:1810	pH. Our findings	1795:1810	From a genetic point of view, the DSCC was positively weakly associated with milk yield, lactose percentage, and milk urea nitrogen, and negatively associated with pH. Our findings contributed to the understanding of the genetic background of DSCC and are a precursor to the potential use of DSCC in breeding programs to enhance cow resistance to mastitis.
30827547	1	97	theme	indicator	208:216	arg1	variation					170:178	genetic variation	162:178	genetic variation	162:178	The aim of the present study was to assess genetic variation and heritability of a novel indicator of udder health, milk differential somatic cell count (DSCC), which represents the percentage of neutrophils plus lymphocytes in the total somatic cell count (SCC).
30827547	1	97	theme	indicator	208:216	arg1	heritability					184:195	heritability	184:195	heritability of a novel indicator of udder health, milk differential somatic cell count (DSCC)	184:277	The aim of the present study was to assess genetic variation and heritability of a novel indicator of udder health, milk differential somatic cell count (DSCC), which represents the percentage of neutrophils plus lymphocytes in the total somatic cell count (SCC).
30678955	0	0	theme	tissue	108:113	arg1	engineering					115:125	modular bone tissue engineering	95:125	modular bone tissue engineering	95:125	Nano-hydroxyapatite-alginate-gelatin microcapsule as a potential osteogenic building block for modular bone tissue engineering.
30678955	1	1	theme	osteoblast-like	395:409	arg1	behavior					416:423	microencapsulated osteoblast-like cell behavior	377:423	microencapsulated osteoblast-like cell behavior	377:423	To develop osteogenic building blocks for modular bone tissue engineering applications, influence of gelatin as cell adhesive molecule and nano-hydroxyapatite (nHA) as osteoconductive component was examined on alginate-based hydrogel properties and microencapsulated osteoblast-like cell behavior by using factorial experimental design technique.
30678955	1	2	theme	tissue	183:188	arg1	applications					202:213	modular bone tissue engineering applications	170:213	modular bone tissue engineering applications	170:213	To develop osteogenic building blocks for modular bone tissue engineering applications, influence of gelatin as cell adhesive molecule and nano-hydroxyapatite (nHA) as osteoconductive component was examined on alginate-based hydrogel properties and microencapsulated osteoblast-like cell behavior by using factorial experimental design technique.
30678955	6	3	theme	gelatin	1116:1122	arg1	concentration					1124:1136	gelatin concentration	1116:1136	gelatin concentration of microcapsules	1116:1153	Proliferation and osteogenic function of the cells fluctuated with increasing gelatin concentration of microcapsules in the presence of nHA, demonstrating that hydrogel properties should be balanced to provide an efficient 3D osteoconductive microcapsule.
30678955	0	4	theme	bone	103:106	arg1	engineering					115:125	modular bone tissue engineering	95:125	modular bone tissue engineering	95:125	Nano-hydroxyapatite-alginate-gelatin microcapsule as a potential osteogenic building block for modular bone tissue engineering.
30678955	1	5	theme	cell	411:414	arg1	behavior					416:423	microencapsulated osteoblast-like cell behavior	377:423	microencapsulated osteoblast-like cell behavior	377:423	To develop osteogenic building blocks for modular bone tissue engineering applications, influence of gelatin as cell adhesive molecule and nano-hydroxyapatite (nHA) as osteoconductive component was examined on alginate-based hydrogel properties and microencapsulated osteoblast-like cell behavior by using factorial experimental design technique.
30678955	5	6	theme	interaction	999:1009	arg1	effect					1011:1016	interaction effect	999:1016	interaction effect of gelatin and nHA	999:1035	The results and their statistical analysis also revealed the importance of interaction effect of gelatin and nHA.
30678955	1	7	theme	engineering	190:200	arg1	applications					202:213	modular bone tissue engineering applications	170:213	modular bone tissue engineering applications	170:213	To develop osteogenic building blocks for modular bone tissue engineering applications, influence of gelatin as cell adhesive molecule and nano-hydroxyapatite (nHA) as osteoconductive component was examined on alginate-based hydrogel properties and microencapsulated osteoblast-like cell behavior by using factorial experimental design technique.
30678955	2	8	theme	mechanical	590:599	arg1	strength					601:608	mechanical strength	590:608	mechanical strength	590:608	nHA and alginate showed a statistically significant impact on swelling reduction, and improvement of stability and mechanical strength of hydrogels, respectively.
30678955	2	9	theme	hydrogels	613:621	arg1	stability					576:584	stability	576:584	stability	576:584	nHA and alginate showed a statistically significant impact on swelling reduction, and improvement of stability and mechanical strength of hydrogels, respectively.
30678955	2	9	theme	hydrogels	613:621	arg1	strength					601:608	mechanical strength	590:608	mechanical strength	590:608	nHA and alginate showed a statistically significant impact on swelling reduction, and improvement of stability and mechanical strength of hydrogels, respectively.
30678955	2	10	from	impact	527:532	arg1	reduction					546:554	swelling reduction	537:554	swelling reduction	537:554	nHA and alginate showed a statistically significant impact on swelling reduction, and improvement of stability and mechanical strength of hydrogels, respectively.
30678955	7	11	theme	ratio	1401:1405	arg1	modulus					1364:1370	compressive modulus	1352:1370	compressive modulus of 0.19 MPa ± 0.02, swelling ratio of 52% ± 8 (24 h) and degradation rate of 12% ± 4 (96 h)	1352:1462	Alginate (1%)-gelatin (2.5%)-nHA (0.5%) microcapsule with compressive modulus of 0.19 MPa ± 0.02, swelling ratio of 52% ± 8 (24 h) and degradation rate of 12% ± 4 (96 h) revealed a maximum performance for the cell proliferation and function, indicating a potential microcapsule composition to prepare building blocks for modular bone tissue engineering.
30678955	6	12	theme	3D	1261:1262	arg1	microcapsule					1280:1291	an efficient 3D osteoconductive microcapsule	1248:1291	an efficient 3D osteoconductive microcapsule	1248:1291	Proliferation and osteogenic function of the cells fluctuated with increasing gelatin concentration of microcapsules in the presence of nHA, demonstrating that hydrogel properties should be balanced to provide an efficient 3D osteoconductive microcapsule.
30678955	7	13	theme	-gelatin	1307:1314	arg1	microcapsule					1334:1345	Alginate (1%)-gelatin (2.5%)-nHA (0.5%) microcapsule	1294:1345	Alginate (1%)-gelatin (2.5%)-nHA (0.5%) microcapsule with compressive modulus of 0.19 MPa ± 0.02, swelling ratio of 52% ± 8 (24 h) and degradation rate of 12% ± 4 (96 h)	1294:1462	Alginate (1%)-gelatin (2.5%)-nHA (0.5%) microcapsule with compressive modulus of 0.19 MPa ± 0.02, swelling ratio of 52% ± 8 (24 h) and degradation rate of 12% ± 4 (96 h) revealed a maximum performance for the cell proliferation and function, indicating a potential microcapsule composition to prepare building blocks for modular bone tissue engineering.
30678955	7	14	theme	maximum	1475:1481	arg1	performance					1483:1493	a maximum performance	1473:1493	a maximum performance for the cell proliferation	1473:1520	Alginate (1%)-gelatin (2.5%)-nHA (0.5%) microcapsule with compressive modulus of 0.19 MPa ± 0.02, swelling ratio of 52% ± 8 (24 h) and degradation rate of 12% ± 4 (96 h) revealed a maximum performance for the cell proliferation and function, indicating a potential microcapsule composition to prepare building blocks for modular bone tissue engineering.
30678955	7	15	with	microcapsule	1334:1345	arg1	modulus					1364:1370	compressive modulus	1352:1370	compressive modulus of 0.19 MPa ± 0.02, swelling ratio of 52% ± 8 (24 h) and degradation rate of 12% ± 4 (96 h)	1352:1462	Alginate (1%)-gelatin (2.5%)-nHA (0.5%) microcapsule with compressive modulus of 0.19 MPa ± 0.02, swelling ratio of 52% ± 8 (24 h) and degradation rate of 12% ± 4 (96 h) revealed a maximum performance for the cell proliferation and function, indicating a potential microcapsule composition to prepare building blocks for modular bone tissue engineering.
30678955	3	16	theme	Gelatin	638:644	arg1	influence					646:654	Gelatin influence	638:654	Gelatin influence	638:654	Gelatin influence, however, was in a reverse manner.
30678955	1	17	theme	osteoconductive	296:310	arg1	component					312:320	osteoconductive component	296:320	osteoconductive component	296:320	To develop osteogenic building blocks for modular bone tissue engineering applications, influence of gelatin as cell adhesive molecule and nano-hydroxyapatite (nHA) as osteoconductive component was examined on alginate-based hydrogel properties and microencapsulated osteoblast-like cell behavior by using factorial experimental design technique.
30678955	2	18	theme	strength	601:608	arg1	improvement					561:571	improvement	561:571	improvement of stability and mechanical strength of hydrogels	561:621	nHA and alginate showed a statistically significant impact on swelling reduction, and improvement of stability and mechanical strength of hydrogels, respectively.
30678955	4	19	theme	bioactivity	806:816	arg1	improvement					842:852	its bioactivity and mechanical strength improvement	802:852	its bioactivity and mechanical strength improvement of hydrogels to the modulus range of mineralized bone tissue	802:913	nHA played imperative roles in promoting microencapsulated osteoblastic cell proliferation and function due to its bioactivity and mechanical strength improvement of hydrogels to the modulus range of mineralized bone tissue in vivo.
30678955	6	20	theme	hydrogel	1198:1205	arg1	properties					1207:1216	hydrogel properties	1198:1216	hydrogel properties	1198:1216	Proliferation and osteogenic function of the cells fluctuated with increasing gelatin concentration of microcapsules in the presence of nHA, demonstrating that hydrogel properties should be balanced to provide an efficient 3D osteoconductive microcapsule.
30678955	4	21	theme	osteoblastic	750:761	arg1	proliferation					768:780	microencapsulated osteoblastic cell proliferation	732:780	microencapsulated osteoblastic cell proliferation	732:780	nHA played imperative roles in promoting microencapsulated osteoblastic cell proliferation and function due to its bioactivity and mechanical strength improvement of hydrogels to the modulus range of mineralized bone tissue in vivo.
30678955	2	22	theme	swelling	537:544	arg1	reduction					546:554	swelling reduction	537:554	swelling reduction	537:554	nHA and alginate showed a statistically significant impact on swelling reduction, and improvement of stability and mechanical strength of hydrogels, respectively.
30678955	1	23	theme	factorial	434:442	arg1	design					457:462	factorial experimental design	434:462	factorial experimental design technique	434:472	To develop osteogenic building blocks for modular bone tissue engineering applications, influence of gelatin as cell adhesive molecule and nano-hydroxyapatite (nHA) as osteoconductive component was examined on alginate-based hydrogel properties and microencapsulated osteoblast-like cell behavior by using factorial experimental design technique.
30678955	7	24	theme	modular	1615:1621	arg1	engineering					1635:1645	modular bone tissue engineering	1615:1645	modular bone tissue engineering	1615:1645	Alginate (1%)-gelatin (2.5%)-nHA (0.5%) microcapsule with compressive modulus of 0.19 MPa ± 0.02, swelling ratio of 52% ± 8 (24 h) and degradation rate of 12% ± 4 (96 h) revealed a maximum performance for the cell proliferation and function, indicating a potential microcapsule composition to prepare building blocks for modular bone tissue engineering.
30678955	4	25	theme	modulus	874:880	arg1	range					882:886	the modulus range	870:886	the modulus range of mineralized bone tissue	870:913	nHA played imperative roles in promoting microencapsulated osteoblastic cell proliferation and function due to its bioactivity and mechanical strength improvement of hydrogels to the modulus range of mineralized bone tissue in vivo.
30678955	4	26	theme	microencapsulated	732:748	arg1	proliferation					768:780	microencapsulated osteoblastic cell proliferation	732:780	microencapsulated osteoblastic cell proliferation	732:780	nHA played imperative roles in promoting microencapsulated osteoblastic cell proliferation and function due to its bioactivity and mechanical strength improvement of hydrogels to the modulus range of mineralized bone tissue in vivo.
30678955	1	27	theme	experimental	444:455	arg1	design					457:462	factorial experimental design	434:462	factorial experimental design technique	434:472	To develop osteogenic building blocks for modular bone tissue engineering applications, influence of gelatin as cell adhesive molecule and nano-hydroxyapatite (nHA) as osteoconductive component was examined on alginate-based hydrogel properties and microencapsulated osteoblast-like cell behavior by using factorial experimental design technique.
30678955	7	28	theme	-nHA	1322:1325	arg1	microcapsule					1334:1345	Alginate (1%)-gelatin (2.5%)-nHA (0.5%) microcapsule	1294:1345	Alginate (1%)-gelatin (2.5%)-nHA (0.5%) microcapsule with compressive modulus of 0.19 MPa ± 0.02, swelling ratio of 52% ± 8 (24 h) and degradation rate of 12% ± 4 (96 h)	1294:1462	Alginate (1%)-gelatin (2.5%)-nHA (0.5%) microcapsule with compressive modulus of 0.19 MPa ± 0.02, swelling ratio of 52% ± 8 (24 h) and degradation rate of 12% ± 4 (96 h) revealed a maximum performance for the cell proliferation and function, indicating a potential microcapsule composition to prepare building blocks for modular bone tissue engineering.
30678955	7	29	theme	cell	1503:1506	arg1	proliferation					1508:1520	the cell proliferation	1499:1520	the cell proliferation	1499:1520	Alginate (1%)-gelatin (2.5%)-nHA (0.5%) microcapsule with compressive modulus of 0.19 MPa ± 0.02, swelling ratio of 52% ± 8 (24 h) and degradation rate of 12% ± 4 (96 h) revealed a maximum performance for the cell proliferation and function, indicating a potential microcapsule composition to prepare building blocks for modular bone tissue engineering.
30678955	6	30	theme	cells	1083:1087	arg1	Proliferation					1038:1050	Proliferation	1038:1050	Proliferation	1038:1050	Proliferation and osteogenic function of the cells fluctuated with increasing gelatin concentration of microcapsules in the presence of nHA, demonstrating that hydrogel properties should be balanced to provide an efficient 3D osteoconductive microcapsule.
30678955	6	30	theme	cells	1083:1087	arg1	function					1067:1074	osteogenic function	1056:1074	osteogenic function	1056:1074	Proliferation and osteogenic function of the cells fluctuated with increasing gelatin concentration of microcapsules in the presence of nHA, demonstrating that hydrogel properties should be balanced to provide an efficient 3D osteoconductive microcapsule.
30678955	4	31	theme	strength	833:840	arg1	improvement					842:852	its bioactivity and mechanical strength improvement	802:852	its bioactivity and mechanical strength improvement of hydrogels to the modulus range of mineralized bone tissue	802:913	nHA played imperative roles in promoting microencapsulated osteoblastic cell proliferation and function due to its bioactivity and mechanical strength improvement of hydrogels to the modulus range of mineralized bone tissue in vivo.
30678955	4	32	theme	cell	763:766	arg1	proliferation					768:780	microencapsulated osteoblastic cell proliferation	732:780	microencapsulated osteoblastic cell proliferation	732:780	nHA played imperative roles in promoting microencapsulated osteoblastic cell proliferation and function due to its bioactivity and mechanical strength improvement of hydrogels to the modulus range of mineralized bone tissue in vivo.
30678955	2	33	theme	significant	515:525	arg1	impact					527:532	a statistically significant impact	499:532	a statistically significant impact on swelling reduction	499:554	nHA and alginate showed a statistically significant impact on swelling reduction, and improvement of stability and mechanical strength of hydrogels, respectively.
30678955	4	34	theme	hydrogels	857:865	arg1	improvement					842:852	its bioactivity and mechanical strength improvement	802:852	its bioactivity and mechanical strength improvement of hydrogels to the modulus range of mineralized bone tissue	802:913	nHA played imperative roles in promoting microencapsulated osteoblastic cell proliferation and function due to its bioactivity and mechanical strength improvement of hydrogels to the modulus range of mineralized bone tissue in vivo.
30678955	1	35	theme	gelatin	229:235	arg1	influence					216:224	influence	216:224	influence of gelatin as cell adhesive molecule and nano-hydroxyapatite (nHA) as osteoconductive component	216:320	To develop osteogenic building blocks for modular bone tissue engineering applications, influence of gelatin as cell adhesive molecule and nano-hydroxyapatite (nHA) as osteoconductive component was examined on alginate-based hydrogel properties and microencapsulated osteoblast-like cell behavior by using factorial experimental design technique.
30678955	1	36	theme	design	457:462	arg1	technique					464:472	factorial experimental design technique	434:472	factorial experimental design technique	434:472	To develop osteogenic building blocks for modular bone tissue engineering applications, influence of gelatin as cell adhesive molecule and nano-hydroxyapatite (nHA) as osteoconductive component was examined on alginate-based hydrogel properties and microencapsulated osteoblast-like cell behavior by using factorial experimental design technique.
30678955	5	37	theme	effect	1011:1016	arg1	importance					985:994	the importance	981:994	the importance of interaction effect of gelatin and nHA	981:1035	The results and their statistical analysis also revealed the importance of interaction effect of gelatin and nHA.
30678955	4	38	theme	imperative	702:711	arg1	roles					713:717	imperative roles	702:717	imperative roles	702:717	nHA played imperative roles in promoting microencapsulated osteoblastic cell proliferation and function due to its bioactivity and mechanical strength improvement of hydrogels to the modulus range of mineralized bone tissue in vivo.
30678955	0	39	theme	osteogenic	65:74	arg1	block					85:89	a potential osteogenic building block	53:89	a potential osteogenic building block for modular bone tissue engineering	53:125	Nano-hydroxyapatite-alginate-gelatin microcapsule as a potential osteogenic building block for modular bone tissue engineering.
30678955	5	40	theme	gelatin	1021:1027	arg1	effect					1011:1016	interaction effect	999:1016	interaction effect of gelatin and nHA	999:1035	The results and their statistical analysis also revealed the importance of interaction effect of gelatin and nHA.
30678955	7	41	theme	0.19 MPa ± 0.02	1375:1389	arg1	modulus					1364:1370	compressive modulus	1352:1370	compressive modulus of 0.19 MPa ± 0.02, swelling ratio of 52% ± 8 (24 h) and degradation rate of 12% ± 4 (96 h)	1352:1462	Alginate (1%)-gelatin (2.5%)-nHA (0.5%) microcapsule with compressive modulus of 0.19 MPa ± 0.02, swelling ratio of 52% ± 8 (24 h) and degradation rate of 12% ± 4 (96 h) revealed a maximum performance for the cell proliferation and function, indicating a potential microcapsule composition to prepare building blocks for modular bone tissue engineering.
30678955	7	42	theme	%	1451:1451	arg1	96 h					1458:1461	96 h	1458:1461	96 h	1458:1461	Alginate (1%)-gelatin (2.5%)-nHA (0.5%) microcapsule with compressive modulus of 0.19 MPa ± 0.02, swelling ratio of 52% ± 8 (24 h) and degradation rate of 12% ± 4 (96 h) revealed a maximum performance for the cell proliferation and function, indicating a potential microcapsule composition to prepare building blocks for modular bone tissue engineering.
30678955	7	42	theme	%	1451:1451	arg1	 ± 4					1452:1455	12% ± 4	1449:1455	12% ± 4 (96 h)	1449:1462	Alginate (1%)-gelatin (2.5%)-nHA (0.5%) microcapsule with compressive modulus of 0.19 MPa ± 0.02, swelling ratio of 52% ± 8 (24 h) and degradation rate of 12% ± 4 (96 h) revealed a maximum performance for the cell proliferation and function, indicating a potential microcapsule composition to prepare building blocks for modular bone tissue engineering.
30678955	7	43	theme	52	1410:1411	arg1	%					1412:1412	%	1412:1412	%	1412:1412	Alginate (1%)-gelatin (2.5%)-nHA (0.5%) microcapsule with compressive modulus of 0.19 MPa ± 0.02, swelling ratio of 52% ± 8 (24 h) and degradation rate of 12% ± 4 (96 h) revealed a maximum performance for the cell proliferation and function, indicating a potential microcapsule composition to prepare building blocks for modular bone tissue engineering.
30678955	1	44	theme	cell	240:243	arg1	molecule					254:261	cell adhesive molecule	240:261	cell adhesive molecule	240:261	To develop osteogenic building blocks for modular bone tissue engineering applications, influence of gelatin as cell adhesive molecule and nano-hydroxyapatite (nHA) as osteoconductive component was examined on alginate-based hydrogel properties and microencapsulated osteoblast-like cell behavior by using factorial experimental design technique.
30678955	0	45	theme	potential	55:63	arg1	block					85:89	a potential osteogenic building block	53:89	a potential osteogenic building block for modular bone tissue engineering	53:125	Nano-hydroxyapatite-alginate-gelatin microcapsule as a potential osteogenic building block for modular bone tissue engineering.
30678955	1	46	theme	alginate-based	338:351	arg1	properties					362:371	alginate-based hydrogel properties	338:371	alginate-based hydrogel properties	338:371	To develop osteogenic building blocks for modular bone tissue engineering applications, influence of gelatin as cell adhesive molecule and nano-hydroxyapatite (nHA) as osteoconductive component was examined on alginate-based hydrogel properties and microencapsulated osteoblast-like cell behavior by using factorial experimental design technique.
30678955	7	47	theme	bone	1623:1626	arg1	engineering					1635:1645	modular bone tissue engineering	1615:1645	modular bone tissue engineering	1615:1645	Alginate (1%)-gelatin (2.5%)-nHA (0.5%) microcapsule with compressive modulus of 0.19 MPa ± 0.02, swelling ratio of 52% ± 8 (24 h) and degradation rate of 12% ± 4 (96 h) revealed a maximum performance for the cell proliferation and function, indicating a potential microcapsule composition to prepare building blocks for modular bone tissue engineering.
30678955	7	48	theme	%	1412:1412	arg1	24 h					1419:1422	24 h	1419:1422	24 h	1419:1422	Alginate (1%)-gelatin (2.5%)-nHA (0.5%) microcapsule with compressive modulus of 0.19 MPa ± 0.02, swelling ratio of 52% ± 8 (24 h) and degradation rate of 12% ± 4 (96 h) revealed a maximum performance for the cell proliferation and function, indicating a potential microcapsule composition to prepare building blocks for modular bone tissue engineering.
30678955	7	48	theme	%	1412:1412	arg1	 ± 8					1413:1416	52% ± 8	1410:1416	52% ± 8 (24 h)	1410:1423	Alginate (1%)-gelatin (2.5%)-nHA (0.5%) microcapsule with compressive modulus of 0.19 MPa ± 0.02, swelling ratio of 52% ± 8 (24 h) and degradation rate of 12% ± 4 (96 h) revealed a maximum performance for the cell proliferation and function, indicating a potential microcapsule composition to prepare building blocks for modular bone tissue engineering.
30678955	3	49	theme	reverse	675:681	arg1	manner					683:688	a reverse manner	673:688	a reverse manner	673:688	Gelatin influence, however, was in a reverse manner.
30678955	7	50	theme	compressive	1352:1362	arg1	modulus					1364:1370	compressive modulus	1352:1370	compressive modulus of 0.19 MPa ± 0.02, swelling ratio of 52% ± 8 (24 h) and degradation rate of 12% ± 4 (96 h)	1352:1462	Alginate (1%)-gelatin (2.5%)-nHA (0.5%) microcapsule with compressive modulus of 0.19 MPa ± 0.02, swelling ratio of 52% ± 8 (24 h) and degradation rate of 12% ± 4 (96 h) revealed a maximum performance for the cell proliferation and function, indicating a potential microcapsule composition to prepare building blocks for modular bone tissue engineering.
30678955	1	51	theme	adhesive	245:252	arg1	molecule					254:261	cell adhesive molecule	240:261	cell adhesive molecule	240:261	To develop osteogenic building blocks for modular bone tissue engineering applications, influence of gelatin as cell adhesive molecule and nano-hydroxyapatite (nHA) as osteoconductive component was examined on alginate-based hydrogel properties and microencapsulated osteoblast-like cell behavior by using factorial experimental design technique.
30678955	1	52	theme	hydrogel	353:360	arg1	properties					362:371	alginate-based hydrogel properties	338:371	alginate-based hydrogel properties	338:371	To develop osteogenic building blocks for modular bone tissue engineering applications, influence of gelatin as cell adhesive molecule and nano-hydroxyapatite (nHA) as osteoconductive component was examined on alginate-based hydrogel properties and microencapsulated osteoblast-like cell behavior by using factorial experimental design technique.
30678955	7	53	theme	degradation	1429:1439	arg1	rate					1441:1444	degradation rate	1429:1444	degradation rate of 12% ± 4 (96 h)	1429:1462	Alginate (1%)-gelatin (2.5%)-nHA (0.5%) microcapsule with compressive modulus of 0.19 MPa ± 0.02, swelling ratio of 52% ± 8 (24 h) and degradation rate of 12% ± 4 (96 h) revealed a maximum performance for the cell proliferation and function, indicating a potential microcapsule composition to prepare building blocks for modular bone tissue engineering.
30678955	1	54	theme	osteogenic	139:148	arg1	blocks					159:164	osteogenic building blocks	139:164	osteogenic building blocks for modular bone tissue engineering applications	139:213	To develop osteogenic building blocks for modular bone tissue engineering applications, influence of gelatin as cell adhesive molecule and nano-hydroxyapatite (nHA) as osteoconductive component was examined on alginate-based hydrogel properties and microencapsulated osteoblast-like cell behavior by using factorial experimental design technique.
30678955	0	55	theme	building	76:83	arg1	block					85:89	a potential osteogenic building block	53:89	a potential osteogenic building block for modular bone tissue engineering	53:125	Nano-hydroxyapatite-alginate-gelatin microcapsule as a potential osteogenic building block for modular bone tissue engineering.
30678955	7	56	dep	-gelatin	1307:1314	arg1	%					1320:1320	2.5%	1317:1320	2.5%	1317:1320	Alginate (1%)-gelatin (2.5%)-nHA (0.5%) microcapsule with compressive modulus of 0.19 MPa ± 0.02, swelling ratio of 52% ± 8 (24 h) and degradation rate of 12% ± 4 (96 h) revealed a maximum performance for the cell proliferation and function, indicating a potential microcapsule composition to prepare building blocks for modular bone tissue engineering.
30678955	7	56	dep	-gelatin	1307:1314	arg1	%					1305:1305	1%	1304:1305	1%	1304:1305	Alginate (1%)-gelatin (2.5%)-nHA (0.5%) microcapsule with compressive modulus of 0.19 MPa ± 0.02, swelling ratio of 52% ± 8 (24 h) and degradation rate of 12% ± 4 (96 h) revealed a maximum performance for the cell proliferation and function, indicating a potential microcapsule composition to prepare building blocks for modular bone tissue engineering.
30678955	7	57	theme	rate	1441:1444	arg1	modulus					1364:1370	compressive modulus	1352:1370	compressive modulus of 0.19 MPa ± 0.02, swelling ratio of 52% ± 8 (24 h) and degradation rate of 12% ± 4 (96 h)	1352:1462	Alginate (1%)-gelatin (2.5%)-nHA (0.5%) microcapsule with compressive modulus of 0.19 MPa ± 0.02, swelling ratio of 52% ± 8 (24 h) and degradation rate of 12% ± 4 (96 h) revealed a maximum performance for the cell proliferation and function, indicating a potential microcapsule composition to prepare building blocks for modular bone tissue engineering.
30678955	7	58	theme	 ± 8	1413:1416	arg1	rate					1441:1444	degradation rate	1429:1444	degradation rate of 12% ± 4 (96 h)	1429:1462	Alginate (1%)-gelatin (2.5%)-nHA (0.5%) microcapsule with compressive modulus of 0.19 MPa ± 0.02, swelling ratio of 52% ± 8 (24 h) and degradation rate of 12% ± 4 (96 h) revealed a maximum performance for the cell proliferation and function, indicating a potential microcapsule composition to prepare building blocks for modular bone tissue engineering.
30678955	7	58	theme	 ± 8	1413:1416	arg1	0.19 MPa ± 0.02					1375:1389	0.19 MPa ± 0.02	1375:1389	0.19 MPa ± 0.02	1375:1389	Alginate (1%)-gelatin (2.5%)-nHA (0.5%) microcapsule with compressive modulus of 0.19 MPa ± 0.02, swelling ratio of 52% ± 8 (24 h) and degradation rate of 12% ± 4 (96 h) revealed a maximum performance for the cell proliferation and function, indicating a potential microcapsule composition to prepare building blocks for modular bone tissue engineering.
30678955	7	58	theme	 ± 8	1413:1416	arg1	ratio					1401:1405	swelling ratio	1392:1405	swelling ratio of 52% ± 8 (24 h)	1392:1423	Alginate (1%)-gelatin (2.5%)-nHA (0.5%) microcapsule with compressive modulus of 0.19 MPa ± 0.02, swelling ratio of 52% ± 8 (24 h) and degradation rate of 12% ± 4 (96 h) revealed a maximum performance for the cell proliferation and function, indicating a potential microcapsule composition to prepare building blocks for modular bone tissue engineering.
30678955	1	59	theme	building	150:157	arg1	blocks					159:164	osteogenic building blocks	139:164	osteogenic building blocks for modular bone tissue engineering applications	139:213	To develop osteogenic building blocks for modular bone tissue engineering applications, influence of gelatin as cell adhesive molecule and nano-hydroxyapatite (nHA) as osteoconductive component was examined on alginate-based hydrogel properties and microencapsulated osteoblast-like cell behavior by using factorial experimental design technique.
30678955	7	60	theme	building	1595:1602	arg1	blocks					1604:1609	building blocks	1595:1609	building blocks for modular bone tissue engineering	1595:1645	Alginate (1%)-gelatin (2.5%)-nHA (0.5%) microcapsule with compressive modulus of 0.19 MPa ± 0.02, swelling ratio of 52% ± 8 (24 h) and degradation rate of 12% ± 4 (96 h) revealed a maximum performance for the cell proliferation and function, indicating a potential microcapsule composition to prepare building blocks for modular bone tissue engineering.
30678955	6	61	theme	osteogenic	1056:1065	arg1	function					1067:1074	osteogenic function	1056:1074	osteogenic function	1056:1074	Proliferation and osteogenic function of the cells fluctuated with increasing gelatin concentration of microcapsules in the presence of nHA, demonstrating that hydrogel properties should be balanced to provide an efficient 3D osteoconductive microcapsule.
30678955	7	62	theme	microcapsule	1559:1570	arg1	composition					1572:1582	microcapsule composition	1559:1582	microcapsule composition	1559:1582	Alginate (1%)-gelatin (2.5%)-nHA (0.5%) microcapsule with compressive modulus of 0.19 MPa ± 0.02, swelling ratio of 52% ± 8 (24 h) and degradation rate of 12% ± 4 (96 h) revealed a maximum performance for the cell proliferation and function, indicating a potential microcapsule composition to prepare building blocks for modular bone tissue engineering.
30678955	4	63	theme	mineralized	891:901	arg1	tissue					908:913	mineralized bone tissue	891:913	mineralized bone tissue	891:913	nHA played imperative roles in promoting microencapsulated osteoblastic cell proliferation and function due to its bioactivity and mechanical strength improvement of hydrogels to the modulus range of mineralized bone tissue in vivo.
30678955	7	64	theme	12	1449:1450	arg1	%					1451:1451	%	1451:1451	%	1451:1451	Alginate (1%)-gelatin (2.5%)-nHA (0.5%) microcapsule with compressive modulus of 0.19 MPa ± 0.02, swelling ratio of 52% ± 8 (24 h) and degradation rate of 12% ± 4 (96 h) revealed a maximum performance for the cell proliferation and function, indicating a potential microcapsule composition to prepare building blocks for modular bone tissue engineering.
30678955	4	65	theme	mechanical	822:831	arg1	improvement					842:852	its bioactivity and mechanical strength improvement	802:852	its bioactivity and mechanical strength improvement of hydrogels to the modulus range of mineralized bone tissue	802:913	nHA played imperative roles in promoting microencapsulated osteoblastic cell proliferation and function due to its bioactivity and mechanical strength improvement of hydrogels to the modulus range of mineralized bone tissue in vivo.
30678955	6	66	theme	osteoconductive	1264:1278	arg1	microcapsule					1280:1291	an efficient 3D osteoconductive microcapsule	1248:1291	an efficient 3D osteoconductive microcapsule	1248:1291	Proliferation and osteogenic function of the cells fluctuated with increasing gelatin concentration of microcapsules in the presence of nHA, demonstrating that hydrogel properties should be balanced to provide an efficient 3D osteoconductive microcapsule.
30678955	7	67	theme	 ± 4	1452:1455	arg1	rate					1441:1444	degradation rate	1429:1444	degradation rate of 12% ± 4 (96 h)	1429:1462	Alginate (1%)-gelatin (2.5%)-nHA (0.5%) microcapsule with compressive modulus of 0.19 MPa ± 0.02, swelling ratio of 52% ± 8 (24 h) and degradation rate of 12% ± 4 (96 h) revealed a maximum performance for the cell proliferation and function, indicating a potential microcapsule composition to prepare building blocks for modular bone tissue engineering.
30678955	7	67	theme	 ± 4	1452:1455	arg1	0.19 MPa ± 0.02					1375:1389	0.19 MPa ± 0.02	1375:1389	0.19 MPa ± 0.02	1375:1389	Alginate (1%)-gelatin (2.5%)-nHA (0.5%) microcapsule with compressive modulus of 0.19 MPa ± 0.02, swelling ratio of 52% ± 8 (24 h) and degradation rate of 12% ± 4 (96 h) revealed a maximum performance for the cell proliferation and function, indicating a potential microcapsule composition to prepare building blocks for modular bone tissue engineering.
30678955	7	67	theme	 ± 4	1452:1455	arg1	ratio					1401:1405	swelling ratio	1392:1405	swelling ratio of 52% ± 8 (24 h)	1392:1423	Alginate (1%)-gelatin (2.5%)-nHA (0.5%) microcapsule with compressive modulus of 0.19 MPa ± 0.02, swelling ratio of 52% ± 8 (24 h) and degradation rate of 12% ± 4 (96 h) revealed a maximum performance for the cell proliferation and function, indicating a potential microcapsule composition to prepare building blocks for modular bone tissue engineering.
30678955	5	68	theme	nHA	1033:1035	arg1	effect					1011:1016	interaction effect	999:1016	interaction effect of gelatin and nHA	999:1035	The results and their statistical analysis also revealed the importance of interaction effect of gelatin and nHA.
30678955	6	69	theme	efficient	1251:1259	arg1	microcapsule					1280:1291	an efficient 3D osteoconductive microcapsule	1248:1291	an efficient 3D osteoconductive microcapsule	1248:1291	Proliferation and osteogenic function of the cells fluctuated with increasing gelatin concentration of microcapsules in the presence of nHA, demonstrating that hydrogel properties should be balanced to provide an efficient 3D osteoconductive microcapsule.
30678955	6	70	theme	microcapsules	1141:1153	arg1	concentration					1124:1136	gelatin concentration	1116:1136	gelatin concentration of microcapsules	1116:1153	Proliferation and osteogenic function of the cells fluctuated with increasing gelatin concentration of microcapsules in the presence of nHA, demonstrating that hydrogel properties should be balanced to provide an efficient 3D osteoconductive microcapsule.
30678955	0	71	theme	modular	95:101	arg1	engineering					115:125	modular bone tissue engineering	95:125	modular bone tissue engineering	95:125	Nano-hydroxyapatite-alginate-gelatin microcapsule as a potential osteogenic building block for modular bone tissue engineering.
30678955	5	72	theme	statistical	946:956	arg1	analysis					958:965	their statistical analysis	940:965	their statistical analysis	940:965	The results and their statistical analysis also revealed the importance of interaction effect of gelatin and nHA.
30678955	4	73	theme	tissue	908:913	arg1	range					882:886	the modulus range	870:886	the modulus range of mineralized bone tissue	870:913	nHA played imperative roles in promoting microencapsulated osteoblastic cell proliferation and function due to its bioactivity and mechanical strength improvement of hydrogels to the modulus range of mineralized bone tissue in vivo.
30678955	6	74	theme	nHA	1174:1176	arg1	presence					1162:1169	the presence	1158:1169	the presence of nHA	1158:1176	Proliferation and osteogenic function of the cells fluctuated with increasing gelatin concentration of microcapsules in the presence of nHA, demonstrating that hydrogel properties should be balanced to provide an efficient 3D osteoconductive microcapsule.
30678955	1	75	theme	modular	170:176	arg1	applications					202:213	modular bone tissue engineering applications	170:213	modular bone tissue engineering applications	170:213	To develop osteogenic building blocks for modular bone tissue engineering applications, influence of gelatin as cell adhesive molecule and nano-hydroxyapatite (nHA) as osteoconductive component was examined on alginate-based hydrogel properties and microencapsulated osteoblast-like cell behavior by using factorial experimental design technique.
30678955	7	76	theme	swelling	1392:1399	arg1	ratio					1401:1405	swelling ratio	1392:1405	swelling ratio of 52% ± 8 (24 h)	1392:1423	Alginate (1%)-gelatin (2.5%)-nHA (0.5%) microcapsule with compressive modulus of 0.19 MPa ± 0.02, swelling ratio of 52% ± 8 (24 h) and degradation rate of 12% ± 4 (96 h) revealed a maximum performance for the cell proliferation and function, indicating a potential microcapsule composition to prepare building blocks for modular bone tissue engineering.
30678955	7	77	theme	tissue	1628:1633	arg1	engineering					1635:1645	modular bone tissue engineering	1615:1645	modular bone tissue engineering	1615:1645	Alginate (1%)-gelatin (2.5%)-nHA (0.5%) microcapsule with compressive modulus of 0.19 MPa ± 0.02, swelling ratio of 52% ± 8 (24 h) and degradation rate of 12% ± 4 (96 h) revealed a maximum performance for the cell proliferation and function, indicating a potential microcapsule composition to prepare building blocks for modular bone tissue engineering.
30678955	2	78	theme	stability	576:584	arg1	improvement					561:571	improvement	561:571	improvement of stability and mechanical strength of hydrogels	561:621	nHA and alginate showed a statistically significant impact on swelling reduction, and improvement of stability and mechanical strength of hydrogels, respectively.
30678955	1	79	theme	microencapsulated	377:393	arg1	behavior					416:423	microencapsulated osteoblast-like cell behavior	377:423	microencapsulated osteoblast-like cell behavior	377:423	To develop osteogenic building blocks for modular bone tissue engineering applications, influence of gelatin as cell adhesive molecule and nano-hydroxyapatite (nHA) as osteoconductive component was examined on alginate-based hydrogel properties and microencapsulated osteoblast-like cell behavior by using factorial experimental design technique.
30678955	4	80	theme	bone	903:906	arg1	tissue					908:913	mineralized bone tissue	891:913	mineralized bone tissue	891:913	nHA played imperative roles in promoting microencapsulated osteoblastic cell proliferation and function due to its bioactivity and mechanical strength improvement of hydrogels to the modulus range of mineralized bone tissue in vivo.
30678955	1	81	theme	bone	178:181	arg1	applications					202:213	modular bone tissue engineering applications	170:213	modular bone tissue engineering applications	170:213	To develop osteogenic building blocks for modular bone tissue engineering applications, influence of gelatin as cell adhesive molecule and nano-hydroxyapatite (nHA) as osteoconductive component was examined on alginate-based hydrogel properties and microencapsulated osteoblast-like cell behavior by using factorial experimental design technique.
29735048	3	0	theme	yellow	513:518	arg1	YM					527:528	YM	527:528	YM	527:528	Flours from three different pigmented maize varieties were evaluated under cooking conditions and compared to common yellow maize (YM).
29735048	3	0	theme	yellow	513:518	arg1	maize					520:524	common yellow maize	506:524	common yellow maize (YM)	506:529	Flours from three different pigmented maize varieties were evaluated under cooking conditions and compared to common yellow maize (YM).
29735048	6	1	theme	following	1108:1116	arg1	metabolomics					1118:1129	multivariate statistics following metabolomics	1084:1129	multivariate statistics following metabolomics	1084:1129	Coherently, multivariate statistics following metabolomics showed the discrimination potential of anthocyanins' profile after cooking, characterising the "Rostrato Rosso" during in vitro digestion.
29735048	4	2	theme	phenolic	612:619	arg1	compounds					621:629	300 phenolic compounds	608:629	300 phenolic compounds	608:629	The untargeted metabolomics-based approach comprehensively annotated around 300 phenolic compounds, with a high distribution of anthocyanins and phenolic acids (in free and bound fractions of maize samples) and significant differences across genotypes.
29735048	6	3	theme	multivariate	1084:1095	arg1	metabolomics					1118:1129	multivariate statistics following metabolomics	1084:1129	multivariate statistics following metabolomics	1084:1129	Coherently, multivariate statistics following metabolomics showed the discrimination potential of anthocyanins' profile after cooking, characterising the "Rostrato Rosso" during in vitro digestion.
29735048	4	4	theme	phenolic	677:684	arg1	acids					686:690	phenolic acids	677:690	phenolic acids	677:690	The untargeted metabolomics-based approach comprehensively annotated around 300 phenolic compounds, with a high distribution of anthocyanins and phenolic acids (in free and bound fractions of maize samples) and significant differences across genotypes.
29735048	5	5	theme	starch	880:885	arg1	content					887:893	higher resistant starch content	863:893	higher resistant starch content (from 5.1 to 6.9 g /100 g dry matter)	863:931	Following in vitro starch digestion, the cooked pigmented maize flours showed higher resistant starch content (from 5.1 to 6.9 g /100 g dry matter), as well as lower starch hydrolysis index (HI) when compared to YM flour, with the "Rostrato Rosso" maize having the lowest HI (i.e., 61).
29735048	5	5	theme	starch	880:885	arg1	matter					925:930	from 5.1 to 6.9 g /100 g dry matter	896:930	from 5.1 to 6.9 g /100 g dry matter	896:930	Following in vitro starch digestion, the cooked pigmented maize flours showed higher resistant starch content (from 5.1 to 6.9 g /100 g dry matter), as well as lower starch hydrolysis index (HI) when compared to YM flour, with the "Rostrato Rosso" maize having the lowest HI (i.e., 61).
29735048	5	6	dep	in	795:796	arg1	vitro					798:802	vitro	798:802	vitro	798:802	Following in vitro starch digestion, the cooked pigmented maize flours showed higher resistant starch content (from 5.1 to 6.9 g /100 g dry matter), as well as lower starch hydrolysis index (HI) when compared to YM flour, with the "Rostrato Rosso" maize having the lowest HI (i.e., 61).
29735048	4	7	theme	metabolomics-based	547:564	arg1	approach					566:573	The untargeted metabolomics-based approach	532:573	The untargeted metabolomics-based approach	532:573	The untargeted metabolomics-based approach comprehensively annotated around 300 phenolic compounds, with a high distribution of anthocyanins and phenolic acids (in free and bound fractions of maize samples) and significant differences across genotypes.
29735048	2	8	theme	phenolic	287:294	arg1	compounds					296:304	phenolic compounds	287:304	phenolic compounds characterising pigmented maize flours	287:342	The main purpose of this study was to investigate the role of phenolic compounds characterising pigmented maize flours in the modulation of in vitro starch digestibility.
29735048	4	9	from	anthocyanins	660:671	arg1	fractions					711:719	free and bound fractions	696:719	free and bound fractions of maize samples	696:736	The untargeted metabolomics-based approach comprehensively annotated around 300 phenolic compounds, with a high distribution of anthocyanins and phenolic acids (in free and bound fractions of maize samples) and significant differences across genotypes.
29735048	6	10	dep	in	1250:1251	arg1	vitro					1253:1257	vitro	1253:1257	vitro	1253:1257	Coherently, multivariate statistics following metabolomics showed the discrimination potential of anthocyanins' profile after cooking, characterising the "Rostrato Rosso" during in vitro digestion.
29735048	7	11	theme	enzyme	1323:1328	arg1	activity					1330:1337	enzyme activity	1323:1337	enzyme activity	1323:1337	These findings might be related to the modulation of enzyme activity by phenolic compounds during in vitro digestion.
29735048	4	12	from	distribution	644:655	arg1	fractions					711:719	free and bound fractions	696:719	free and bound fractions of maize samples	696:736	The untargeted metabolomics-based approach comprehensively annotated around 300 phenolic compounds, with a high distribution of anthocyanins and phenolic acids (in free and bound fractions of maize samples) and significant differences across genotypes.
29735048	1	13	theme	digestive	192:200	arg1	enzymes					202:208	starch digestive enzymes	185:208	starch digestive enzymes	185:208	Interest in using polyphenols as modulators of the activity of starch digestive enzymes is increasing.
29735048	3	14	theme	pigmented	424:432	arg1	varieties					440:448	three different pigmented maize varieties	408:448	three different pigmented maize varieties	408:448	Flours from three different pigmented maize varieties were evaluated under cooking conditions and compared to common yellow maize (YM).
29735048	4	15	theme	anthocyanins	660:671	arg1	distribution					644:655	a high distribution	637:655	a high distribution of anthocyanins and phenolic acids (in free and bound fractions of maize samples) and significant differences across genotypes	637:782	The untargeted metabolomics-based approach comprehensively annotated around 300 phenolic compounds, with a high distribution of anthocyanins and phenolic acids (in free and bound fractions of maize samples) and significant differences across genotypes.
29735048	5	16	theme	Rosso	1026:1030	arg1	maize					1033:1037	the "Rostrato Rosso" maize	1012:1037	the "Rostrato Rosso" maize having the lowest HI (i.e., 61)	1012:1069	Following in vitro starch digestion, the cooked pigmented maize flours showed higher resistant starch content (from 5.1 to 6.9 g /100 g dry matter), as well as lower starch hydrolysis index (HI) when compared to YM flour, with the "Rostrato Rosso" maize having the lowest HI (i.e., 61).
29735048	5	17	theme	cooked	826:831	arg1	flours					849:854	the cooked pigmented maize flours	822:854	the cooked pigmented maize flours	822:854	Following in vitro starch digestion, the cooked pigmented maize flours showed higher resistant starch content (from 5.1 to 6.9 g /100 g dry matter), as well as lower starch hydrolysis index (HI) when compared to YM flour, with the "Rostrato Rosso" maize having the lowest HI (i.e., 61).
29735048	5	18	theme	higher	863:868	arg1	content					887:893	higher resistant starch content	863:893	higher resistant starch content (from 5.1 to 6.9 g /100 g dry matter)	863:931	Following in vitro starch digestion, the cooked pigmented maize flours showed higher resistant starch content (from 5.1 to 6.9 g /100 g dry matter), as well as lower starch hydrolysis index (HI) when compared to YM flour, with the "Rostrato Rosso" maize having the lowest HI (i.e., 61).
29735048	5	18	theme	higher	863:868	arg1	matter					925:930	from 5.1 to 6.9 g /100 g dry matter	896:930	from 5.1 to 6.9 g /100 g dry matter	896:930	Following in vitro starch digestion, the cooked pigmented maize flours showed higher resistant starch content (from 5.1 to 6.9 g /100 g dry matter), as well as lower starch hydrolysis index (HI) when compared to YM flour, with the "Rostrato Rosso" maize having the lowest HI (i.e., 61).
29735048	8	19	theme	gluten-free	1466:1476	arg1	foods					1478:1482	gluten-free foods	1466:1482	gluten-free foods with slowly digestible starches	1466:1514	Therefore, the use of pigmented maize flours might help in the formulation of gluten-free foods with slowly digestible starches by exploiting the wide phenolic composition of these matrices.
29735048	4	20	with	compounds	621:629	arg1	distribution					644:655	a high distribution	637:655	a high distribution of anthocyanins and phenolic acids (in free and bound fractions of maize samples) and significant differences across genotypes	637:782	The untargeted metabolomics-based approach comprehensively annotated around 300 phenolic compounds, with a high distribution of anthocyanins and phenolic acids (in free and bound fractions of maize samples) and significant differences across genotypes.
29735048	5	21	theme	g	919:919	arg1	content					887:893	higher resistant starch content	863:893	higher resistant starch content (from 5.1 to 6.9 g /100 g dry matter)	863:931	Following in vitro starch digestion, the cooked pigmented maize flours showed higher resistant starch content (from 5.1 to 6.9 g /100 g dry matter), as well as lower starch hydrolysis index (HI) when compared to YM flour, with the "Rostrato Rosso" maize having the lowest HI (i.e., 61).
29735048	5	21	theme	g	919:919	arg1	matter					925:930	from 5.1 to 6.9 g /100 g dry matter	896:930	from 5.1 to 6.9 g /100 g dry matter	896:930	Following in vitro starch digestion, the cooked pigmented maize flours showed higher resistant starch content (from 5.1 to 6.9 g /100 g dry matter), as well as lower starch hydrolysis index (HI) when compared to YM flour, with the "Rostrato Rosso" maize having the lowest HI (i.e., 61).
29735048	6	22	theme	discrimination	1142:1155	arg1	potential					1157:1165	the discrimination potential	1138:1165	the discrimination potential of anthocyanins' profile	1138:1190	Coherently, multivariate statistics following metabolomics showed the discrimination potential of anthocyanins' profile after cooking, characterising the "Rostrato Rosso" during in vitro digestion.
29735048	4	23	theme	samples	730:736	arg1	fractions					711:719	free and bound fractions	696:719	free and bound fractions of maize samples	696:736	The untargeted metabolomics-based approach comprehensively annotated around 300 phenolic compounds, with a high distribution of anthocyanins and phenolic acids (in free and bound fractions of maize samples) and significant differences across genotypes.
29735048	6	24	theme	Rosso	1236:1240	arg1	"					1241:1241	the "Rostrato Rosso"	1222:1241	the "Rostrato Rosso"	1222:1241	Coherently, multivariate statistics following metabolomics showed the discrimination potential of anthocyanins' profile after cooking, characterising the "Rostrato Rosso" during in vitro digestion.
29735048	7	25	theme	in	1368:1369	arg1	digestion					1377:1385	in vitro digestion	1368:1385	in vitro digestion	1368:1385	These findings might be related to the modulation of enzyme activity by phenolic compounds during in vitro digestion.
29735048	5	26	theme	dry	921:923	arg1	content					887:893	higher resistant starch content	863:893	higher resistant starch content (from 5.1 to 6.9 g /100 g dry matter)	863:931	Following in vitro starch digestion, the cooked pigmented maize flours showed higher resistant starch content (from 5.1 to 6.9 g /100 g dry matter), as well as lower starch hydrolysis index (HI) when compared to YM flour, with the "Rostrato Rosso" maize having the lowest HI (i.e., 61).
29735048	5	26	theme	dry	921:923	arg1	matter					925:930	from 5.1 to 6.9 g /100 g dry matter	896:930	from 5.1 to 6.9 g /100 g dry matter	896:930	Following in vitro starch digestion, the cooked pigmented maize flours showed higher resistant starch content (from 5.1 to 6.9 g /100 g dry matter), as well as lower starch hydrolysis index (HI) when compared to YM flour, with the "Rostrato Rosso" maize having the lowest HI (i.e., 61).
29735048	4	27	theme	free	696:699	arg1	fractions					711:719	free and bound fractions	696:719	free and bound fractions of maize samples	696:736	The untargeted metabolomics-based approach comprehensively annotated around 300 phenolic compounds, with a high distribution of anthocyanins and phenolic acids (in free and bound fractions of maize samples) and significant differences across genotypes.
29735048	0	28	theme	starch	46:51	arg1	digestibility					53:65	the in vitro starch digestibility	33:65	the in vitro starch digestibility of pigmented maize	33:84	Effect of dietary polyphenols on the in vitro starch digestibility of pigmented maize varieties under cooking conditions.
29735048	5	29	theme	pigmented	833:841	arg1	flours					849:854	the cooked pigmented maize flours	822:854	the cooked pigmented maize flours	822:854	Following in vitro starch digestion, the cooked pigmented maize flours showed higher resistant starch content (from 5.1 to 6.9 g /100 g dry matter), as well as lower starch hydrolysis index (HI) when compared to YM flour, with the "Rostrato Rosso" maize having the lowest HI (i.e., 61).
29735048	8	30	theme	maize	1420:1424	arg1	flours					1426:1431	pigmented maize flours	1410:1431	pigmented maize flours	1410:1431	Therefore, the use of pigmented maize flours might help in the formulation of gluten-free foods with slowly digestible starches by exploiting the wide phenolic composition of these matrices.
29735048	4	31	theme	bound	705:709	arg1	fractions					711:719	free and bound fractions	696:719	free and bound fractions of maize samples	696:736	The untargeted metabolomics-based approach comprehensively annotated around 300 phenolic compounds, with a high distribution of anthocyanins and phenolic acids (in free and bound fractions of maize samples) and significant differences across genotypes.
29735048	0	32	from	Effect	0:5	arg1	digestibility					53:65	the in vitro starch digestibility	33:65	the in vitro starch digestibility of pigmented maize	33:84	Effect of dietary polyphenols on the in vitro starch digestibility of pigmented maize varieties under cooking conditions.
29735048	0	33	theme	maize	80:84	arg1	digestibility					53:65	the in vitro starch digestibility	33:65	the in vitro starch digestibility of pigmented maize	33:84	Effect of dietary polyphenols on the in vitro starch digestibility of pigmented maize varieties under cooking conditions.
29735048	5	34	theme	lower	945:949	arg1	HI					976:977	HI	976:977	HI	976:977	Following in vitro starch digestion, the cooked pigmented maize flours showed higher resistant starch content (from 5.1 to 6.9 g /100 g dry matter), as well as lower starch hydrolysis index (HI) when compared to YM flour, with the "Rostrato Rosso" maize having the lowest HI (i.e., 61).
29735048	5	34	theme	lower	945:949	arg1	index					969:973	lower starch hydrolysis index	945:973	lower starch hydrolysis index (HI)	945:978	Following in vitro starch digestion, the cooked pigmented maize flours showed higher resistant starch content (from 5.1 to 6.9 g /100 g dry matter), as well as lower starch hydrolysis index (HI) when compared to YM flour, with the "Rostrato Rosso" maize having the lowest HI (i.e., 61).
29735048	5	35	contain	having	1039:1044	arg2	61					1067:1068	61	1067:1068	61	1067:1068	Following in vitro starch digestion, the cooked pigmented maize flours showed higher resistant starch content (from 5.1 to 6.9 g /100 g dry matter), as well as lower starch hydrolysis index (HI) when compared to YM flour, with the "Rostrato Rosso" maize having the lowest HI (i.e., 61).
29735048	5	35	contain	having	1039:1044	arg1	maize					1033:1037	the "Rostrato Rosso" maize	1012:1037	the "Rostrato Rosso" maize having the lowest HI (i.e., 61)	1012:1069	Following in vitro starch digestion, the cooked pigmented maize flours showed higher resistant starch content (from 5.1 to 6.9 g /100 g dry matter), as well as lower starch hydrolysis index (HI) when compared to YM flour, with the "Rostrato Rosso" maize having the lowest HI (i.e., 61).
29735048	5	35	contain	having	1039:1044	arg2	HI					1057:1058	the lowest HI	1046:1058	the lowest HI (i.e., 61)	1046:1069	Following in vitro starch digestion, the cooked pigmented maize flours showed higher resistant starch content (from 5.1 to 6.9 g /100 g dry matter), as well as lower starch hydrolysis index (HI) when compared to YM flour, with the "Rostrato Rosso" maize having the lowest HI (i.e., 61).
29735048	2	36	theme	digestibility	381:393	arg1	modulation					351:360	the modulation	347:360	the modulation of in vitro starch digestibility	347:393	The main purpose of this study was to investigate the role of phenolic compounds characterising pigmented maize flours in the modulation of in vitro starch digestibility.
29735048	4	37	from	differences	755:765	arg1	fractions					711:719	free and bound fractions	696:719	free and bound fractions of maize samples	696:736	The untargeted metabolomics-based approach comprehensively annotated around 300 phenolic compounds, with a high distribution of anthocyanins and phenolic acids (in free and bound fractions of maize samples) and significant differences across genotypes.
29735048	5	38	theme	hydrolysis	958:967	arg1	HI					976:977	HI	976:977	HI	976:977	Following in vitro starch digestion, the cooked pigmented maize flours showed higher resistant starch content (from 5.1 to 6.9 g /100 g dry matter), as well as lower starch hydrolysis index (HI) when compared to YM flour, with the "Rostrato Rosso" maize having the lowest HI (i.e., 61).
29735048	5	38	theme	hydrolysis	958:967	arg1	index					969:973	lower starch hydrolysis index	945:973	lower starch hydrolysis index (HI)	945:978	Following in vitro starch digestion, the cooked pigmented maize flours showed higher resistant starch content (from 5.1 to 6.9 g /100 g dry matter), as well as lower starch hydrolysis index (HI) when compared to YM flour, with the "Rostrato Rosso" maize having the lowest HI (i.e., 61).
29735048	3	39	theme	different	414:422	arg1	varieties					440:448	three different pigmented maize varieties	408:448	three different pigmented maize varieties	408:448	Flours from three different pigmented maize varieties were evaluated under cooking conditions and compared to common yellow maize (YM).
29735048	4	40	theme	untargeted	536:545	arg1	approach					566:573	The untargeted metabolomics-based approach	532:573	The untargeted metabolomics-based approach	532:573	The untargeted metabolomics-based approach comprehensively annotated around 300 phenolic compounds, with a high distribution of anthocyanins and phenolic acids (in free and bound fractions of maize samples) and significant differences across genotypes.
29735048	4	41	theme	differences	755:765	arg1	distribution					644:655	a high distribution	637:655	a high distribution of anthocyanins and phenolic acids (in free and bound fractions of maize samples) and significant differences across genotypes	637:782	The untargeted metabolomics-based approach comprehensively annotated around 300 phenolic compounds, with a high distribution of anthocyanins and phenolic acids (in free and bound fractions of maize samples) and significant differences across genotypes.
29735048	0	42	theme	dietary	10:16	arg1	polyphenols					18:28	dietary polyphenols	10:28	dietary polyphenols	10:28	Effect of dietary polyphenols on the in vitro starch digestibility of pigmented maize varieties under cooking conditions.
29735048	0	43	dep	in	37:38	arg1	vitro					40:44	vitro	40:44	vitro	40:44	Effect of dietary polyphenols on the in vitro starch digestibility of pigmented maize varieties under cooking conditions.
29735048	8	44	theme	digestible	1496:1505	arg1	starches					1507:1514	slowly digestible starches	1489:1514	slowly digestible starches	1489:1514	Therefore, the use of pigmented maize flours might help in the formulation of gluten-free foods with slowly digestible starches by exploiting the wide phenolic composition of these matrices.
29735048	0	45	theme	in	37:38	arg1	digestibility					53:65	the in vitro starch digestibility	33:65	the in vitro starch digestibility of pigmented maize	33:84	Effect of dietary polyphenols on the in vitro starch digestibility of pigmented maize varieties under cooking conditions.
29735048	5	46	theme	YM	997:998	arg1	flour					1000:1004	YM flour	997:1004	YM flour	997:1004	Following in vitro starch digestion, the cooked pigmented maize flours showed higher resistant starch content (from 5.1 to 6.9 g /100 g dry matter), as well as lower starch hydrolysis index (HI) when compared to YM flour, with the "Rostrato Rosso" maize having the lowest HI (i.e., 61).
29735048	2	47	theme	pigmented	321:329	arg1	flours					337:342	pigmented maize flours	321:342	pigmented maize flours	321:342	The main purpose of this study was to investigate the role of phenolic compounds characterising pigmented maize flours in the modulation of in vitro starch digestibility.
29735048	4	48	from	fractions	711:719	arg1	distribution					644:655	a high distribution	637:655	a high distribution of anthocyanins and phenolic acids (in free and bound fractions of maize samples) and significant differences across genotypes	637:782	The untargeted metabolomics-based approach comprehensively annotated around 300 phenolic compounds, with a high distribution of anthocyanins and phenolic acids (in free and bound fractions of maize samples) and significant differences across genotypes.
29735048	8	49	theme	phenolic	1539:1546	arg1	composition					1548:1558	the wide phenolic composition	1530:1558	the wide phenolic composition of these matrices	1530:1576	Therefore, the use of pigmented maize flours might help in the formulation of gluten-free foods with slowly digestible starches by exploiting the wide phenolic composition of these matrices.
29735048	6	50	theme	Rostrato	1227:1234	arg1	"					1241:1241	the "Rostrato Rosso"	1222:1241	the "Rostrato Rosso"	1222:1241	Coherently, multivariate statistics following metabolomics showed the discrimination potential of anthocyanins' profile after cooking, characterising the "Rostrato Rosso" during in vitro digestion.
29735048	3	51	from	varieties	440:448	arg1	Flours					396:401	Flours	396:401	Flours from three different pigmented maize varieties	396:448	Flours from three different pigmented maize varieties were evaluated under cooking conditions and compared to common yellow maize (YM).
29735048	3	52	theme	common	506:511	arg1	YM					527:528	YM	527:528	YM	527:528	Flours from three different pigmented maize varieties were evaluated under cooking conditions and compared to common yellow maize (YM).
29735048	3	52	theme	common	506:511	arg1	maize					520:524	common yellow maize	506:524	common yellow maize (YM)	506:529	Flours from three different pigmented maize varieties were evaluated under cooking conditions and compared to common yellow maize (YM).
29735048	6	53	theme	statistics	1097:1106	arg1	metabolomics					1118:1129	multivariate statistics following metabolomics	1084:1129	multivariate statistics following metabolomics	1084:1129	Coherently, multivariate statistics following metabolomics showed the discrimination potential of anthocyanins' profile after cooking, characterising the "Rostrato Rosso" during in vitro digestion.
29735048	8	54	theme	matrices	1569:1576	arg1	composition					1548:1558	the wide phenolic composition	1530:1558	the wide phenolic composition of these matrices	1530:1576	Therefore, the use of pigmented maize flours might help in the formulation of gluten-free foods with slowly digestible starches by exploiting the wide phenolic composition of these matrices.
29735048	4	55	from	acids	686:690	arg1	fractions					711:719	free and bound fractions	696:719	free and bound fractions of maize samples	696:736	The untargeted metabolomics-based approach comprehensively annotated around 300 phenolic compounds, with a high distribution of anthocyanins and phenolic acids (in free and bound fractions of maize samples) and significant differences across genotypes.
29735048	5	56	theme	resistant	870:878	arg1	content					887:893	higher resistant starch content	863:893	higher resistant starch content (from 5.1 to 6.9 g /100 g dry matter)	863:931	Following in vitro starch digestion, the cooked pigmented maize flours showed higher resistant starch content (from 5.1 to 6.9 g /100 g dry matter), as well as lower starch hydrolysis index (HI) when compared to YM flour, with the "Rostrato Rosso" maize having the lowest HI (i.e., 61).
29735048	5	56	theme	resistant	870:878	arg1	matter					925:930	from 5.1 to 6.9 g /100 g dry matter	896:930	from 5.1 to 6.9 g /100 g dry matter	896:930	Following in vitro starch digestion, the cooked pigmented maize flours showed higher resistant starch content (from 5.1 to 6.9 g /100 g dry matter), as well as lower starch hydrolysis index (HI) when compared to YM flour, with the "Rostrato Rosso" maize having the lowest HI (i.e., 61).
29735048	2	57	theme	compounds	296:304	arg1	role					279:282	the role	275:282	the role of phenolic compounds characterising pigmented maize flours in the modulation of in vitro starch digestibility	275:393	The main purpose of this study was to investigate the role of phenolic compounds characterising pigmented maize flours in the modulation of in vitro starch digestibility.
29735048	1	58	theme	activity	173:180	arg1	polyphenols					140:150	polyphenols	140:150	polyphenols	140:150	Interest in using polyphenols as modulators of the activity of starch digestive enzymes is increasing.
29735048	1	58	theme	activity	173:180	arg1	modulators					155:164	modulators	155:164	modulators of the activity of starch digestive enzymes	155:208	Interest in using polyphenols as modulators of the activity of starch digestive enzymes is increasing.
29735048	7	59	theme	phenolic	1342:1349	arg1	compounds					1351:1359	phenolic compounds	1342:1359	phenolic compounds	1342:1359	These findings might be related to the modulation of enzyme activity by phenolic compounds during in vitro digestion.
29735048	4	60	theme	acids	686:690	arg1	distribution					644:655	a high distribution	637:655	a high distribution of anthocyanins and phenolic acids (in free and bound fractions of maize samples) and significant differences across genotypes	637:782	The untargeted metabolomics-based approach comprehensively annotated around 300 phenolic compounds, with a high distribution of anthocyanins and phenolic acids (in free and bound fractions of maize samples) and significant differences across genotypes.
29735048	1	61	theme	starch	185:190	arg1	enzymes					202:208	starch digestive enzymes	185:208	starch digestive enzymes	185:208	Interest in using polyphenols as modulators of the activity of starch digestive enzymes is increasing.
29735048	7	62	theme	activity	1330:1337	arg1	modulation					1309:1318	the modulation	1305:1318	the modulation of enzyme activity by phenolic compounds	1305:1359	These findings might be related to the modulation of enzyme activity by phenolic compounds during in vitro digestion.
29735048	4	63	theme	high	639:642	arg1	distribution					644:655	a high distribution	637:655	a high distribution of anthocyanins and phenolic acids (in free and bound fractions of maize samples) and significant differences across genotypes	637:782	The untargeted metabolomics-based approach comprehensively annotated around 300 phenolic compounds, with a high distribution of anthocyanins and phenolic acids (in free and bound fractions of maize samples) and significant differences across genotypes.
29735048	2	64	theme	main	229:232	arg1	purpose					234:240	The main purpose	225:240	The main purpose of this study	225:254	The main purpose of this study was to investigate the role of phenolic compounds characterising pigmented maize flours in the modulation of in vitro starch digestibility.
29735048	1	65	theme	enzymes	202:208	arg1	activity					173:180	the activity	169:180	the activity of starch digestive enzymes	169:208	Interest in using polyphenols as modulators of the activity of starch digestive enzymes is increasing.
29735048	6	66	theme	in	1250:1251	arg1	digestion					1259:1267	in vitro digestion	1250:1267	in vitro digestion	1250:1267	Coherently, multivariate statistics following metabolomics showed the discrimination potential of anthocyanins' profile after cooking, characterising the "Rostrato Rosso" during in vitro digestion.
29735048	5	67	theme	"	1031:1031	arg1	maize					1033:1037	the "Rostrato Rosso" maize	1012:1037	the "Rostrato Rosso" maize having the lowest HI (i.e., 61)	1012:1069	Following in vitro starch digestion, the cooked pigmented maize flours showed higher resistant starch content (from 5.1 to 6.9 g /100 g dry matter), as well as lower starch hydrolysis index (HI) when compared to YM flour, with the "Rostrato Rosso" maize having the lowest HI (i.e., 61).
29735048	8	68	with	foods	1478:1482	arg1	starches					1507:1514	slowly digestible starches	1489:1514	slowly digestible starches	1489:1514	Therefore, the use of pigmented maize flours might help in the formulation of gluten-free foods with slowly digestible starches by exploiting the wide phenolic composition of these matrices.
29735048	2	69	theme	study	250:254	arg1	purpose					234:240	The main purpose	225:240	The main purpose of this study	225:254	The main purpose of this study was to investigate the role of phenolic compounds characterising pigmented maize flours in the modulation of in vitro starch digestibility.
29735048	7	70	dep	in	1368:1369	arg1	vitro					1371:1375	vitro	1371:1375	vitro	1371:1375	These findings might be related to the modulation of enzyme activity by phenolic compounds during in vitro digestion.
29735048	8	71	theme	pigmented	1410:1418	arg1	flours					1426:1431	pigmented maize flours	1410:1431	pigmented maize flours	1410:1431	Therefore, the use of pigmented maize flours might help in the formulation of gluten-free foods with slowly digestible starches by exploiting the wide phenolic composition of these matrices.
29735048	4	72	theme	maize	724:728	arg1	samples					730:736	maize samples	724:736	maize samples	724:736	The untargeted metabolomics-based approach comprehensively annotated around 300 phenolic compounds, with a high distribution of anthocyanins and phenolic acids (in free and bound fractions of maize samples) and significant differences across genotypes.
29735048	5	73	theme	maize	843:847	arg1	flours					849:854	the cooked pigmented maize flours	822:854	the cooked pigmented maize flours	822:854	Following in vitro starch digestion, the cooked pigmented maize flours showed higher resistant starch content (from 5.1 to 6.9 g /100 g dry matter), as well as lower starch hydrolysis index (HI) when compared to YM flour, with the "Rostrato Rosso" maize having the lowest HI (i.e., 61).
29735048	8	74	theme	flours	1426:1431	arg1	use					1403:1405	the use	1399:1405	the use of pigmented maize flours	1399:1431	Therefore, the use of pigmented maize flours might help in the formulation of gluten-free foods with slowly digestible starches by exploiting the wide phenolic composition of these matrices.
29735048	0	75	theme	pigmented	70:78	arg1	maize					80:84	pigmented maize	70:84	pigmented maize	70:84	Effect of dietary polyphenols on the in vitro starch digestibility of pigmented maize varieties under cooking conditions.
29735048	2	76	from	role	279:282	arg1	modulation					351:360	the modulation	347:360	the modulation of in vitro starch digestibility	347:393	The main purpose of this study was to investigate the role of phenolic compounds characterising pigmented maize flours in the modulation of in vitro starch digestibility.
29735048	0	77	theme	cooking	102:108	arg1	conditions					110:119	cooking conditions	102:119	cooking conditions	102:119	Effect of dietary polyphenols on the in vitro starch digestibility of pigmented maize varieties under cooking conditions.
29735048	5	78	theme	starch	951:956	arg1	HI					976:977	HI	976:977	HI	976:977	Following in vitro starch digestion, the cooked pigmented maize flours showed higher resistant starch content (from 5.1 to 6.9 g /100 g dry matter), as well as lower starch hydrolysis index (HI) when compared to YM flour, with the "Rostrato Rosso" maize having the lowest HI (i.e., 61).
29735048	5	78	theme	starch	951:956	arg1	index					969:973	lower starch hydrolysis index	945:973	lower starch hydrolysis index (HI)	945:978	Following in vitro starch digestion, the cooked pigmented maize flours showed higher resistant starch content (from 5.1 to 6.9 g /100 g dry matter), as well as lower starch hydrolysis index (HI) when compared to YM flour, with the "Rostrato Rosso" maize having the lowest HI (i.e., 61).
29735048	5	79	dep	61	1067:1068	arg1	i.e.					1061:1064	i.e.	1061:1064	i.e.	1061:1064	Following in vitro starch digestion, the cooked pigmented maize flours showed higher resistant starch content (from 5.1 to 6.9 g /100 g dry matter), as well as lower starch hydrolysis index (HI) when compared to YM flour, with the "Rostrato Rosso" maize having the lowest HI (i.e., 61).
29735048	2	80	theme	starch	374:379	arg1	digestibility					381:393	in vitro starch digestibility	365:393	in vitro starch digestibility	365:393	The main purpose of this study was to investigate the role of phenolic compounds characterising pigmented maize flours in the modulation of in vitro starch digestibility.
29735048	5	81	theme	lowest	1050:1055	arg1	61					1067:1068	61	1067:1068	61	1067:1068	Following in vitro starch digestion, the cooked pigmented maize flours showed higher resistant starch content (from 5.1 to 6.9 g /100 g dry matter), as well as lower starch hydrolysis index (HI) when compared to YM flour, with the "Rostrato Rosso" maize having the lowest HI (i.e., 61).
29735048	5	81	theme	lowest	1050:1055	arg1	HI					1057:1058	the lowest HI	1046:1058	the lowest HI (i.e., 61)	1046:1069	Following in vitro starch digestion, the cooked pigmented maize flours showed higher resistant starch content (from 5.1 to 6.9 g /100 g dry matter), as well as lower starch hydrolysis index (HI) when compared to YM flour, with the "Rostrato Rosso" maize having the lowest HI (i.e., 61).
29735048	8	82	theme	foods	1478:1482	arg1	formulation					1451:1461	the formulation	1447:1461	the formulation of gluten-free foods with slowly digestible starches	1447:1514	Therefore, the use of pigmented maize flours might help in the formulation of gluten-free foods with slowly digestible starches by exploiting the wide phenolic composition of these matrices.
29735048	5	83	dep	6.9 g	908:912	arg1	to					905:906	to	905:906	to	905:906	Following in vitro starch digestion, the cooked pigmented maize flours showed higher resistant starch content (from 5.1 to 6.9 g /100 g dry matter), as well as lower starch hydrolysis index (HI) when compared to YM flour, with the "Rostrato Rosso" maize having the lowest HI (i.e., 61).
29735048	2	84	theme	in	365:366	arg1	digestibility					381:393	in vitro starch digestibility	365:393	in vitro starch digestibility	365:393	The main purpose of this study was to investigate the role of phenolic compounds characterising pigmented maize flours in the modulation of in vitro starch digestibility.
29735048	0	85	theme	polyphenols	18:28	arg1	Effect					0:5	Effect	0:5	Effect of dietary polyphenols on the in vitro starch digestibility of pigmented maize	0:84	Effect of dietary polyphenols on the in vitro starch digestibility of pigmented maize varieties under cooking conditions.
29735048	4	86	theme	significant	743:753	arg1	differences					755:765	significant differences	743:765	significant differences across genotypes	743:782	The untargeted metabolomics-based approach comprehensively annotated around 300 phenolic compounds, with a high distribution of anthocyanins and phenolic acids (in free and bound fractions of maize samples) and significant differences across genotypes.
29735048	3	87	theme	cooking	471:477	arg1	conditions					479:488	cooking conditions	471:488	cooking conditions	471:488	Flours from three different pigmented maize varieties were evaluated under cooking conditions and compared to common yellow maize (YM).
29735048	5	88	theme	in	795:796	arg1	digestion					811:819	in vitro starch digestion	795:819	in vitro starch digestion	795:819	Following in vitro starch digestion, the cooked pigmented maize flours showed higher resistant starch content (from 5.1 to 6.9 g /100 g dry matter), as well as lower starch hydrolysis index (HI) when compared to YM flour, with the "Rostrato Rosso" maize having the lowest HI (i.e., 61).
29735048	3	89	theme	maize	434:438	arg1	varieties					440:448	three different pigmented maize varieties	408:448	three different pigmented maize varieties	408:448	Flours from three different pigmented maize varieties were evaluated under cooking conditions and compared to common yellow maize (YM).
29735048	5	90	theme	starch	804:809	arg1	digestion					811:819	in vitro starch digestion	795:819	in vitro starch digestion	795:819	Following in vitro starch digestion, the cooked pigmented maize flours showed higher resistant starch content (from 5.1 to 6.9 g /100 g dry matter), as well as lower starch hydrolysis index (HI) when compared to YM flour, with the "Rostrato Rosso" maize having the lowest HI (i.e., 61).
29735048	2	91	theme	maize	331:335	arg1	flours					337:342	pigmented maize flours	321:342	pigmented maize flours	321:342	The main purpose of this study was to investigate the role of phenolic compounds characterising pigmented maize flours in the modulation of in vitro starch digestibility.
29735048	8	92	theme	wide	1534:1537	arg1	composition					1548:1558	the wide phenolic composition	1530:1558	the wide phenolic composition of these matrices	1530:1576	Therefore, the use of pigmented maize flours might help in the formulation of gluten-free foods with slowly digestible starches by exploiting the wide phenolic composition of these matrices.
29735048	2	93	dep	in	365:366	arg1	vitro					368:372	vitro	368:372	vitro	368:372	The main purpose of this study was to investigate the role of phenolic compounds characterising pigmented maize flours in the modulation of in vitro starch digestibility.
29354845	3	0	from	chains	533:538	arg1	solution					571:578	aqueous solution	563:578	aqueous solution	563:578	AgNP hybrid supramolecular hydrogels were thus prepared through host-guest inclusion between PEGMA side chains and α-cyclodextrins in aqueous solution.
29354845	2	1	theme	polyacrylic	351:361	arg1	acid					363:366	polyacrylic acid	351:366	polyacrylic acid	351:366	Herein, PEGylated AgNPs were prepared in situ by complexing AgNO3 with the random copolymer of poly(ethylene glycol) methyl ether methacrylate (PEGMA) and polyacrylic acid via electrostatic interaction followed by in situ reduction.
29354845	2	2	theme	glycol	305:310	arg1	PEGMA					340:344	PEGMA	340:344	PEGMA	340:344	Herein, PEGylated AgNPs were prepared in situ by complexing AgNO3 with the random copolymer of poly(ethylene glycol) methyl ether methacrylate (PEGMA) and polyacrylic acid via electrostatic interaction followed by in situ reduction.
29354845	2	2	theme	glycol	305:310	arg1	methacrylate					326:337	poly(ethylene glycol) methyl ether methacrylate	291:337	poly(ethylene glycol) methyl ether methacrylate (PEGMA)	291:345	Herein, PEGylated AgNPs were prepared in situ by complexing AgNO3 with the random copolymer of poly(ethylene glycol) methyl ether methacrylate (PEGMA) and polyacrylic acid via electrostatic interaction followed by in situ reduction.
29354845	2	3	theme	methacrylate	326:337	arg1	copolymer					278:286	the random copolymer	267:286	the random copolymer of poly(ethylene glycol) methyl ether methacrylate (PEGMA)	267:345	Herein, PEGylated AgNPs were prepared in situ by complexing AgNO3 with the random copolymer of poly(ethylene glycol) methyl ether methacrylate (PEGMA) and polyacrylic acid via electrostatic interaction followed by in situ reduction.
29354845	3	4	from	α-cyclodextrins	544:558	arg1	solution					571:578	aqueous solution	563:578	aqueous solution	563:578	AgNP hybrid supramolecular hydrogels were thus prepared through host-guest inclusion between PEGMA side chains and α-cyclodextrins in aqueous solution.
29354845	2	5	theme	ethylene	296:303	arg1	PEGMA					340:344	PEGMA	340:344	PEGMA	340:344	Herein, PEGylated AgNPs were prepared in situ by complexing AgNO3 with the random copolymer of poly(ethylene glycol) methyl ether methacrylate (PEGMA) and polyacrylic acid via electrostatic interaction followed by in situ reduction.
29354845	2	5	theme	ethylene	296:303	arg1	methacrylate					326:337	poly(ethylene glycol) methyl ether methacrylate	291:337	poly(ethylene glycol) methyl ether methacrylate (PEGMA)	291:345	Herein, PEGylated AgNPs were prepared in situ by complexing AgNO3 with the random copolymer of poly(ethylene glycol) methyl ether methacrylate (PEGMA) and polyacrylic acid via electrostatic interaction followed by in situ reduction.
29354845	4	6	theme	pseudopolyrotaxane	632:649	arg1	crystallization					651:665	pseudopolyrotaxane crystallization	632:665	pseudopolyrotaxane crystallization	632:665	The hydrogels were physically cross-linked by both pseudopolyrotaxane crystallization and AgNPs, which showed temperature responsiveness and self-healing properties.
29354845	3	7	theme	host-guest	493:502	arg1	inclusion					504:512	host-guest inclusion	493:512	host-guest inclusion between PEGMA side chains and α-cyclodextrins in aqueous solution	493:578	AgNP hybrid supramolecular hydrogels were thus prepared through host-guest inclusion between PEGMA side chains and α-cyclodextrins in aqueous solution.
29354845	3	8	theme	aqueous	563:569	arg1	solution					571:578	aqueous solution	563:578	aqueous solution	563:578	AgNP hybrid supramolecular hydrogels were thus prepared through host-guest inclusion between PEGMA side chains and α-cyclodextrins in aqueous solution.
29354845	2	9	theme	in	410:411	arg1	reduction					418:426	in situ reduction	410:426	in situ reduction	410:426	Herein, PEGylated AgNPs were prepared in situ by complexing AgNO3 with the random copolymer of poly(ethylene glycol) methyl ether methacrylate (PEGMA) and polyacrylic acid via electrostatic interaction followed by in situ reduction.
29354845	2	10	theme	poly	291:294	arg1	PEGMA					340:344	PEGMA	340:344	PEGMA	340:344	Herein, PEGylated AgNPs were prepared in situ by complexing AgNO3 with the random copolymer of poly(ethylene glycol) methyl ether methacrylate (PEGMA) and polyacrylic acid via electrostatic interaction followed by in situ reduction.
29354845	2	10	theme	poly	291:294	arg1	methacrylate					326:337	poly(ethylene glycol) methyl ether methacrylate	291:337	poly(ethylene glycol) methyl ether methacrylate (PEGMA)	291:345	Herein, PEGylated AgNPs were prepared in situ by complexing AgNO3 with the random copolymer of poly(ethylene glycol) methyl ether methacrylate (PEGMA) and polyacrylic acid via electrostatic interaction followed by in situ reduction.
29354845	4	11	theme	self-healing	722:733	arg1	properties					735:744	self-healing properties	722:744	self-healing properties	722:744	The hydrogels were physically cross-linked by both pseudopolyrotaxane crystallization and AgNPs, which showed temperature responsiveness and self-healing properties.
29354845	0	12	theme	injectable	3:12	arg1	hydrogel					29:36	An injectable supramolecular hydrogel	0:36	An injectable supramolecular hydrogel hybridized with silver	0:59	An injectable supramolecular hydrogel hybridized with silver nanoparticles for antibacterial application.
29354845	1	13	theme	Silver	106:111	arg1	nanoparticles					113:125	Silver nanoparticles	106:125	Silver nanoparticles (AgNPs)	106:133	Silver nanoparticles (AgNPs) show long-lasting and broad-spectrum antibacterial activity.
29354845	1	13	theme	Silver	106:111	arg1	AgNPs					128:132	AgNPs	128:132	AgNPs	128:132	Silver nanoparticles (AgNPs) show long-lasting and broad-spectrum antibacterial activity.
29354845	3	14	theme	hybrid	434:439	arg1	hydrogels					456:464	AgNP hybrid supramolecular hydrogels	429:464	AgNP hybrid supramolecular hydrogels	429:464	AgNP hybrid supramolecular hydrogels were thus prepared through host-guest inclusion between PEGMA side chains and α-cyclodextrins in aqueous solution.
29354845	2	15	theme	ether	320:324	arg1	PEGMA					340:344	PEGMA	340:344	PEGMA	340:344	Herein, PEGylated AgNPs were prepared in situ by complexing AgNO3 with the random copolymer of poly(ethylene glycol) methyl ether methacrylate (PEGMA) and polyacrylic acid via electrostatic interaction followed by in situ reduction.
29354845	2	15	theme	ether	320:324	arg1	methacrylate					326:337	poly(ethylene glycol) methyl ether methacrylate	291:337	poly(ethylene glycol) methyl ether methacrylate (PEGMA)	291:345	Herein, PEGylated AgNPs were prepared in situ by complexing AgNO3 with the random copolymer of poly(ethylene glycol) methyl ether methacrylate (PEGMA) and polyacrylic acid via electrostatic interaction followed by in situ reduction.
29354845	3	16	theme	supramolecular	441:454	arg1	hydrogels					456:464	AgNP hybrid supramolecular hydrogels	429:464	AgNP hybrid supramolecular hydrogels	429:464	AgNP hybrid supramolecular hydrogels were thus prepared through host-guest inclusion between PEGMA side chains and α-cyclodextrins in aqueous solution.
29354845	2	17	theme	random	271:276	arg1	copolymer					278:286	the random copolymer	267:286	the random copolymer of poly(ethylene glycol) methyl ether methacrylate (PEGMA)	267:345	Herein, PEGylated AgNPs were prepared in situ by complexing AgNO3 with the random copolymer of poly(ethylene glycol) methyl ether methacrylate (PEGMA) and polyacrylic acid via electrostatic interaction followed by in situ reduction.
29354845	2	18	theme	methyl	313:318	arg1	PEGMA					340:344	PEGMA	340:344	PEGMA	340:344	Herein, PEGylated AgNPs were prepared in situ by complexing AgNO3 with the random copolymer of poly(ethylene glycol) methyl ether methacrylate (PEGMA) and polyacrylic acid via electrostatic interaction followed by in situ reduction.
29354845	2	18	theme	methyl	313:318	arg1	methacrylate					326:337	poly(ethylene glycol) methyl ether methacrylate	291:337	poly(ethylene glycol) methyl ether methacrylate (PEGMA)	291:345	Herein, PEGylated AgNPs were prepared in situ by complexing AgNO3 with the random copolymer of poly(ethylene glycol) methyl ether methacrylate (PEGMA) and polyacrylic acid via electrostatic interaction followed by in situ reduction.
29354845	4	19	theme	temperature	691:701	arg1	responsiveness					703:716	temperature responsiveness	691:716	temperature responsiveness	691:716	The hydrogels were physically cross-linked by both pseudopolyrotaxane crystallization and AgNPs, which showed temperature responsiveness and self-healing properties.
29354845	0	20	theme	supramolecular	14:27	arg1	hydrogel					29:36	An injectable supramolecular hydrogel	0:36	An injectable supramolecular hydrogel hybridized with silver	0:59	An injectable supramolecular hydrogel hybridized with silver nanoparticles for antibacterial application.
29354845	1	21	theme	broad-spectrum	157:170	arg1	activity					186:193	long-lasting and broad-spectrum antibacterial activity	140:193	long-lasting and broad-spectrum antibacterial activity	140:193	Silver nanoparticles (AgNPs) show long-lasting and broad-spectrum antibacterial activity.
29354845	2	22	with	AgNO3	256:260	arg1	copolymer					278:286	the random copolymer	267:286	the random copolymer of poly(ethylene glycol) methyl ether methacrylate (PEGMA)	267:345	Herein, PEGylated AgNPs were prepared in situ by complexing AgNO3 with the random copolymer of poly(ethylene glycol) methyl ether methacrylate (PEGMA) and polyacrylic acid via electrostatic interaction followed by in situ reduction.
29354845	5	23	theme	excellent	790:798	arg1	properties					814:823	excellent antibacterial properties	790:823	excellent antibacterial properties	790:823	By hybridizing AgNPs, the hydrogels showed excellent antibacterial properties against S. aureus and E. coli bacteria as well as low cytotoxicity and have potential applications as injectable antibacterial materials.
29354845	2	24	theme	PEGylated	204:212	arg1	AgNPs					214:218	PEGylated AgNPs	204:218	PEGylated AgNPs	204:218	Herein, PEGylated AgNPs were prepared in situ by complexing AgNO3 with the random copolymer of poly(ethylene glycol) methyl ether methacrylate (PEGMA) and polyacrylic acid via electrostatic interaction followed by in situ reduction.
29354845	5	25	theme	injectable	927:936	arg1	applications					911:922	potential applications	901:922	potential applications	901:922	By hybridizing AgNPs, the hydrogels showed excellent antibacterial properties against S. aureus and E. coli bacteria as well as low cytotoxicity and have potential applications as injectable antibacterial materials.
29354845	5	25	theme	injectable	927:936	arg1	materials					952:960	injectable antibacterial materials	927:960	injectable antibacterial materials	927:960	By hybridizing AgNPs, the hydrogels showed excellent antibacterial properties against S. aureus and E. coli bacteria as well as low cytotoxicity and have potential applications as injectable antibacterial materials.
29354845	3	26	theme	AgNP	429:432	arg1	hydrogels					456:464	AgNP hybrid supramolecular hydrogels	429:464	AgNP hybrid supramolecular hydrogels	429:464	AgNP hybrid supramolecular hydrogels were thus prepared through host-guest inclusion between PEGMA side chains and α-cyclodextrins in aqueous solution.
29354845	5	27	theme	antibacterial	800:812	arg1	properties					814:823	excellent antibacterial properties	790:823	excellent antibacterial properties	790:823	By hybridizing AgNPs, the hydrogels showed excellent antibacterial properties against S. aureus and E. coli bacteria as well as low cytotoxicity and have potential applications as injectable antibacterial materials.
29354845	5	28	theme	antibacterial	938:950	arg1	applications					911:922	potential applications	901:922	potential applications	901:922	By hybridizing AgNPs, the hydrogels showed excellent antibacterial properties against S. aureus and E. coli bacteria as well as low cytotoxicity and have potential applications as injectable antibacterial materials.
29354845	5	28	theme	antibacterial	938:950	arg1	materials					952:960	injectable antibacterial materials	927:960	injectable antibacterial materials	927:960	By hybridizing AgNPs, the hydrogels showed excellent antibacterial properties against S. aureus and E. coli bacteria as well as low cytotoxicity and have potential applications as injectable antibacterial materials.
29354845	3	29	theme	PEGMA	522:526	arg1	chains					533:538	PEGMA side chains	522:538	PEGMA side chains	522:538	AgNP hybrid supramolecular hydrogels were thus prepared through host-guest inclusion between PEGMA side chains and α-cyclodextrins in aqueous solution.
29354845	5	30	theme	low	875:877	arg1	cytotoxicity					879:890	low cytotoxicity	875:890	low cytotoxicity	875:890	By hybridizing AgNPs, the hydrogels showed excellent antibacterial properties against S. aureus and E. coli bacteria as well as low cytotoxicity and have potential applications as injectable antibacterial materials.
29354845	5	31	dep	aureus	836:841	arg1	bacteria					855:862	bacteria	855:862	bacteria	855:862	By hybridizing AgNPs, the hydrogels showed excellent antibacterial properties against S. aureus and E. coli bacteria as well as low cytotoxicity and have potential applications as injectable antibacterial materials.
29354845	2	32	with	acid	363:366	arg1	copolymer					278:286	the random copolymer	267:286	the random copolymer of poly(ethylene glycol) methyl ether methacrylate (PEGMA)	267:345	Herein, PEGylated AgNPs were prepared in situ by complexing AgNO3 with the random copolymer of poly(ethylene glycol) methyl ether methacrylate (PEGMA) and polyacrylic acid via electrostatic interaction followed by in situ reduction.
29354845	3	33	theme	side	528:531	arg1	chains					533:538	PEGMA side chains	522:538	PEGMA side chains	522:538	AgNP hybrid supramolecular hydrogels were thus prepared through host-guest inclusion between PEGMA side chains and α-cyclodextrins in aqueous solution.
29354845	1	34	theme	long-lasting	140:151	arg1	activity					186:193	long-lasting and broad-spectrum antibacterial activity	140:193	long-lasting and broad-spectrum antibacterial activity	140:193	Silver nanoparticles (AgNPs) show long-lasting and broad-spectrum antibacterial activity.
29354845	5	35	theme	potential	901:909	arg1	applications					911:922	potential applications	901:922	potential applications	901:922	By hybridizing AgNPs, the hydrogels showed excellent antibacterial properties against S. aureus and E. coli bacteria as well as low cytotoxicity and have potential applications as injectable antibacterial materials.
29354845	5	35	theme	potential	901:909	arg1	materials					952:960	injectable antibacterial materials	927:960	injectable antibacterial materials	927:960	By hybridizing AgNPs, the hydrogels showed excellent antibacterial properties against S. aureus and E. coli bacteria as well as low cytotoxicity and have potential applications as injectable antibacterial materials.
29354845	0	36	theme	antibacterial	79:91	arg1	application					93:103	antibacterial application	79:103	antibacterial application	79:103	An injectable supramolecular hydrogel hybridized with silver nanoparticles for antibacterial application.
29354845	2	37	dep	in	410:411	arg1	situ					413:416	situ	413:416	situ	413:416	Herein, PEGylated AgNPs were prepared in situ by complexing AgNO3 with the random copolymer of poly(ethylene glycol) methyl ether methacrylate (PEGMA) and polyacrylic acid via electrostatic interaction followed by in situ reduction.
29354845	2	38	theme	electrostatic	372:384	arg1	interaction					386:396	electrostatic interaction	372:396	electrostatic interaction followed by in situ reduction	372:426	Herein, PEGylated AgNPs were prepared in situ by complexing AgNO3 with the random copolymer of poly(ethylene glycol) methyl ether methacrylate (PEGMA) and polyacrylic acid via electrostatic interaction followed by in situ reduction.
29354845	5	39	contain	have	896:899	arg2	applications					911:922	potential applications	901:922	potential applications	901:922	By hybridizing AgNPs, the hydrogels showed excellent antibacterial properties against S. aureus and E. coli bacteria as well as low cytotoxicity and have potential applications as injectable antibacterial materials.
29354845	5	39	contain	have	896:899	arg2	materials					952:960	injectable antibacterial materials	927:960	injectable antibacterial materials	927:960	By hybridizing AgNPs, the hydrogels showed excellent antibacterial properties against S. aureus and E. coli bacteria as well as low cytotoxicity and have potential applications as injectable antibacterial materials.
29354845	5	39	contain	have	896:899	arg1	hydrogels					773:781	the hydrogels	769:781	the hydrogels	769:781	By hybridizing AgNPs, the hydrogels showed excellent antibacterial properties against S. aureus and E. coli bacteria as well as low cytotoxicity and have potential applications as injectable antibacterial materials.
29354845	1	40	theme	antibacterial	172:184	arg1	activity					186:193	long-lasting and broad-spectrum antibacterial activity	140:193	long-lasting and broad-spectrum antibacterial activity	140:193	Silver nanoparticles (AgNPs) show long-lasting and broad-spectrum antibacterial activity.
30775747	4	0	theme	particles	773:781	arg1	changes					787:793	changes	787:793	changes in energy gap of NCs	787:814	The PL properties were mainly discussed in terms of the colloidal stability of the particles and changes in energy gap of NCs.
30775747	4	0	theme	particles	773:781	arg1	stability					756:764	the colloidal stability	742:764	the colloidal stability of the particles	742:781	The PL properties were mainly discussed in terms of the colloidal stability of the particles and changes in energy gap of NCs.
30775747	1	1	theme	semiconductor	201:213	arg1	NCs					229:231	NCs	229:231	NCs	229:231	The photoluminescence (PL) properties of composites obtained by embedding green-emitting semiconductor nanocrystals (NCs) of two different types (thiol-capped CdTe and CdSe/ZnS) into chitosan-based biopolymer particles were investigated.
30775747	1	1	theme	semiconductor	201:213	arg1	nanocrystals					215:226	green-emitting semiconductor nanocrystals	186:226	green-emitting semiconductor nanocrystals (NCs) of two different types (thiol-capped CdTe and CdSe/ZnS)	186:288	The photoluminescence (PL) properties of composites obtained by embedding green-emitting semiconductor nanocrystals (NCs) of two different types (thiol-capped CdTe and CdSe/ZnS) into chitosan-based biopolymer particles were investigated.
30775747	5	2	theme	obtained	832:839	arg1	composites					855:864	the obtained biocompatible composites	828:864	the obtained biocompatible composites with NCs randomly distributed within a biopolymer particle	828:923	Generally, the obtained biocompatible composites with NCs randomly distributed within a biopolymer particle demonstrated a higher PL resistance to the solution acidity that expands the applicability range of thiol-capped NCs.
30775747	5	3	theme	biocompatible	841:853	arg1	composites					855:864	the obtained biocompatible composites	828:864	the obtained biocompatible composites with NCs randomly distributed within a biopolymer particle	828:923	Generally, the obtained biocompatible composites with NCs randomly distributed within a biopolymer particle demonstrated a higher PL resistance to the solution acidity that expands the applicability range of thiol-capped NCs.
30775747	3	4	theme	solution	623:630	arg1	amount					586:591	The amount	582:591	The amount of NCs	582:598	The amount of NCs and the acidity of the solution were found to be important parameters influencing the PL.
30775747	3	4	theme	solution	623:630	arg1	solution					623:630	the solution	619:630	the solution	619:630	The amount of NCs and the acidity of the solution were found to be important parameters influencing the PL.
30775747	3	4	theme	solution	623:630	arg1	parameters					659:668	important parameters	649:668	important parameters influencing the PL	649:687	The amount of NCs and the acidity of the solution were found to be important parameters influencing the PL.
30775747	3	4	theme	solution	623:630	arg1	NCs					596:598	NCs	596:598	NCs	596:598	The amount of NCs and the acidity of the solution were found to be important parameters influencing the PL.
30775747	3	4	theme	solution	623:630	arg1	acidity					608:614	the acidity	604:614	the acidity of the solution	604:630	The amount of NCs and the acidity of the solution were found to be important parameters influencing the PL.
30775747	4	5	from	stability	756:764	arg1	gap					805:807	energy gap	798:807	energy gap of NCs	798:814	The PL properties were mainly discussed in terms of the colloidal stability of the particles and changes in energy gap of NCs.
30775747	1	6	theme	chitosan-based	295:308	arg1	particles					321:329	chitosan-based biopolymer particles	295:329	chitosan-based biopolymer particles	295:329	The photoluminescence (PL) properties of composites obtained by embedding green-emitting semiconductor nanocrystals (NCs) of two different types (thiol-capped CdTe and CdSe/ZnS) into chitosan-based biopolymer particles were investigated.
30775747	3	7	theme	NCs	596:598	arg1	amount					586:591	The amount	582:591	The amount of NCs	582:598	The amount of NCs and the acidity of the solution were found to be important parameters influencing the PL.
30775747	3	7	theme	NCs	596:598	arg1	solution					623:630	the solution	619:630	the solution	619:630	The amount of NCs and the acidity of the solution were found to be important parameters influencing the PL.
30775747	3	7	theme	NCs	596:598	arg1	parameters					659:668	important parameters	649:668	important parameters influencing the PL	649:687	The amount of NCs and the acidity of the solution were found to be important parameters influencing the PL.
30775747	3	7	theme	NCs	596:598	arg1	NCs					596:598	NCs	596:598	NCs	596:598	The amount of NCs and the acidity of the solution were found to be important parameters influencing the PL.
30775747	3	7	theme	NCs	596:598	arg1	acidity					608:614	the acidity	604:614	the acidity of the solution	604:630	The amount of NCs and the acidity of the solution were found to be important parameters influencing the PL.
30775747	1	8	theme	biopolymer	310:319	arg1	particles					321:329	chitosan-based biopolymer particles	295:329	chitosan-based biopolymer particles	295:329	The photoluminescence (PL) properties of composites obtained by embedding green-emitting semiconductor nanocrystals (NCs) of two different types (thiol-capped CdTe and CdSe/ZnS) into chitosan-based biopolymer particles were investigated.
30775747	4	9	theme	changes	787:793	arg1	terms					733:737	terms	733:737	terms of the colloidal stability of the particles and changes in energy gap of NCs	733:814	The PL properties were mainly discussed in terms of the colloidal stability of the particles and changes in energy gap of NCs.
30775747	5	10	theme	thiol-capped	1025:1036	arg1	NCs					1038:1040	thiol-capped NCs	1025:1040	thiol-capped NCs	1025:1040	Generally, the obtained biocompatible composites with NCs randomly distributed within a biopolymer particle demonstrated a higher PL resistance to the solution acidity that expands the applicability range of thiol-capped NCs.
30775747	2	11	theme	chains	508:513	arg1	binding					469:475	a preliminary electrostatic binding	441:475	a preliminary electrostatic binding of positively charged chitosan chains by negatively charged functional groups of NC stabilizing ligands	441:579	The synthesis of self-assembled particles from oppositely charged polysaccharides involved a preliminary electrostatic binding of positively charged chitosan chains by negatively charged functional groups of NC stabilizing ligands.
30775747	5	12	theme	higher	940:945	arg1	resistance					950:959	a higher PL resistance	938:959	a higher PL resistance to the solution acidity that expands the applicability range of thiol-capped NCs	938:1040	Generally, the obtained biocompatible composites with NCs randomly distributed within a biopolymer particle demonstrated a higher PL resistance to the solution acidity that expands the applicability range of thiol-capped NCs.
30775747	2	13	theme	ligands	573:579	arg1	groups					548:553	negatively charged functional groups	518:553	negatively charged functional groups of NC stabilizing ligands	518:579	The synthesis of self-assembled particles from oppositely charged polysaccharides involved a preliminary electrostatic binding of positively charged chitosan chains by negatively charged functional groups of NC stabilizing ligands.
30775747	2	13	theme	ligands	573:579	arg1	ligands					573:579	NC stabilizing ligands	558:579	NC stabilizing ligands	558:579	The synthesis of self-assembled particles from oppositely charged polysaccharides involved a preliminary electrostatic binding of positively charged chitosan chains by negatively charged functional groups of NC stabilizing ligands.
30775747	1	14	theme	photoluminescence	116:132	arg1	properties					139:148	The photoluminescence (PL) properties	112:148	The photoluminescence (PL) properties of composites obtained by embedding green-emitting semiconductor nanocrystals (NCs) of two different types (thiol-capped CdTe and CdSe/ZnS) into chitosan-based biopolymer particles	112:329	The photoluminescence (PL) properties of composites obtained by embedding green-emitting semiconductor nanocrystals (NCs) of two different types (thiol-capped CdTe and CdSe/ZnS) into chitosan-based biopolymer particles were investigated.
30775747	5	15	theme	PL	947:948	arg1	resistance					950:959	a higher PL resistance	938:959	a higher PL resistance to the solution acidity that expands the applicability range of thiol-capped NCs	938:1040	Generally, the obtained biocompatible composites with NCs randomly distributed within a biopolymer particle demonstrated a higher PL resistance to the solution acidity that expands the applicability range of thiol-capped NCs.
30775747	2	16	from	polysaccharides	416:430	arg1	synthesis					354:362	The synthesis	350:362	The synthesis of self-assembled particles from oppositely charged polysaccharides	350:430	The synthesis of self-assembled particles from oppositely charged polysaccharides involved a preliminary electrostatic binding of positively charged chitosan chains by negatively charged functional groups of NC stabilizing ligands.
30775747	2	16	from	polysaccharides	416:430	arg1	particles					382:390	self-assembled particles	367:390	self-assembled particles from oppositely charged polysaccharides	367:430	The synthesis of self-assembled particles from oppositely charged polysaccharides involved a preliminary electrostatic binding of positively charged chitosan chains by negatively charged functional groups of NC stabilizing ligands.
30775747	2	17	theme	self-assembled	367:380	arg1	particles					382:390	self-assembled particles	367:390	self-assembled particles from oppositely charged polysaccharides	367:430	The synthesis of self-assembled particles from oppositely charged polysaccharides involved a preliminary electrostatic binding of positively charged chitosan chains by negatively charged functional groups of NC stabilizing ligands.
30775747	4	18	from	terms	733:737	arg1	gap					805:807	energy gap	798:807	energy gap of NCs	798:814	The PL properties were mainly discussed in terms of the colloidal stability of the particles and changes in energy gap of NCs.
30775747	4	19	theme	colloidal	746:754	arg1	stability					756:764	the colloidal stability	742:764	the colloidal stability of the particles	742:781	The PL properties were mainly discussed in terms of the colloidal stability of the particles and changes in energy gap of NCs.
30775747	0	20	theme	Photoluminescence	0:16	arg1	properties					18:27	Photoluminescence properties	0:27	Photoluminescence properties of self-assembled chitosan-based composites	0:71	Photoluminescence properties of self-assembled chitosan-based composites containing semiconductor nanocrystals.
30775747	1	21	theme	PL	135:136	arg1	properties					139:148	The photoluminescence (PL) properties	112:148	The photoluminescence (PL) properties of composites obtained by embedding green-emitting semiconductor nanocrystals (NCs) of two different types (thiol-capped CdTe and CdSe/ZnS) into chitosan-based biopolymer particles	112:329	The photoluminescence (PL) properties of composites obtained by embedding green-emitting semiconductor nanocrystals (NCs) of two different types (thiol-capped CdTe and CdSe/ZnS) into chitosan-based biopolymer particles were investigated.
30775747	2	22	theme	electrostatic	455:467	arg1	binding					469:475	a preliminary electrostatic binding	441:475	a preliminary electrostatic binding of positively charged chitosan chains by negatively charged functional groups of NC stabilizing ligands	441:579	The synthesis of self-assembled particles from oppositely charged polysaccharides involved a preliminary electrostatic binding of positively charged chitosan chains by negatively charged functional groups of NC stabilizing ligands.
30775747	0	23	theme	self-assembled	32:45	arg1	composites					62:71	self-assembled chitosan-based composites	32:71	self-assembled chitosan-based composites	32:71	Photoluminescence properties of self-assembled chitosan-based composites containing semiconductor nanocrystals.
30775747	4	24	from	gap	805:807	arg1	terms					733:737	terms	733:737	terms of the colloidal stability of the particles and changes in energy gap of NCs	733:814	The PL properties were mainly discussed in terms of the colloidal stability of the particles and changes in energy gap of NCs.
30775747	5	25	theme	NCs	1038:1040	arg1	range					1016:1020	the applicability range	998:1020	the applicability range of thiol-capped NCs	998:1040	Generally, the obtained biocompatible composites with NCs randomly distributed within a biopolymer particle demonstrated a higher PL resistance to the solution acidity that expands the applicability range of thiol-capped NCs.
30775747	5	26	theme	biopolymer	905:914	arg1	particle					916:923	a biopolymer particle	903:923	a biopolymer particle	903:923	Generally, the obtained biocompatible composites with NCs randomly distributed within a biopolymer particle demonstrated a higher PL resistance to the solution acidity that expands the applicability range of thiol-capped NCs.
30775747	3	27	theme	important	649:657	arg1	amount					586:591	The amount	582:591	The amount of NCs	582:598	The amount of NCs and the acidity of the solution were found to be important parameters influencing the PL.
30775747	3	27	theme	important	649:657	arg1	solution					623:630	the solution	619:630	the solution	619:630	The amount of NCs and the acidity of the solution were found to be important parameters influencing the PL.
30775747	3	27	theme	important	649:657	arg1	parameters					659:668	important parameters	649:668	important parameters influencing the PL	649:687	The amount of NCs and the acidity of the solution were found to be important parameters influencing the PL.
30775747	3	27	theme	important	649:657	arg1	NCs					596:598	NCs	596:598	NCs	596:598	The amount of NCs and the acidity of the solution were found to be important parameters influencing the PL.
30775747	3	27	theme	important	649:657	arg1	acidity					608:614	the acidity	604:614	the acidity of the solution	604:630	The amount of NCs and the acidity of the solution were found to be important parameters influencing the PL.
30775747	2	28	theme	functional	537:546	arg1	groups					548:553	negatively charged functional groups	518:553	negatively charged functional groups of NC stabilizing ligands	518:579	The synthesis of self-assembled particles from oppositely charged polysaccharides involved a preliminary electrostatic binding of positively charged chitosan chains by negatively charged functional groups of NC stabilizing ligands.
30775747	2	28	theme	functional	537:546	arg1	ligands					573:579	NC stabilizing ligands	558:579	NC stabilizing ligands	558:579	The synthesis of self-assembled particles from oppositely charged polysaccharides involved a preliminary electrostatic binding of positively charged chitosan chains by negatively charged functional groups of NC stabilizing ligands.
30775747	4	29	theme	NCs	812:814	arg1	gap					805:807	energy gap	798:807	energy gap of NCs	798:814	The PL properties were mainly discussed in terms of the colloidal stability of the particles and changes in energy gap of NCs.
30775747	1	30	theme	different	241:249	arg1	CdSe/ZnS					280:287	CdSe/ZnS	280:287	CdSe/ZnS	280:287	The photoluminescence (PL) properties of composites obtained by embedding green-emitting semiconductor nanocrystals (NCs) of two different types (thiol-capped CdTe and CdSe/ZnS) into chitosan-based biopolymer particles were investigated.
30775747	1	30	theme	different	241:249	arg1	types					251:255	two different types	237:255	two different types (thiol-capped CdTe and CdSe/ZnS)	237:288	The photoluminescence (PL) properties of composites obtained by embedding green-emitting semiconductor nanocrystals (NCs) of two different types (thiol-capped CdTe and CdSe/ZnS) into chitosan-based biopolymer particles were investigated.
30775747	1	30	theme	different	241:249	arg1	CdTe					271:274	thiol-capped CdTe	258:274	thiol-capped CdTe	258:274	The photoluminescence (PL) properties of composites obtained by embedding green-emitting semiconductor nanocrystals (NCs) of two different types (thiol-capped CdTe and CdSe/ZnS) into chitosan-based biopolymer particles were investigated.
30775747	0	31	theme	composites	62:71	arg1	properties					18:27	Photoluminescence properties	0:27	Photoluminescence properties of self-assembled chitosan-based composites	0:71	Photoluminescence properties of self-assembled chitosan-based composites containing semiconductor nanocrystals.
30775747	1	32	theme	types	251:255	arg1	NCs					229:231	NCs	229:231	NCs	229:231	The photoluminescence (PL) properties of composites obtained by embedding green-emitting semiconductor nanocrystals (NCs) of two different types (thiol-capped CdTe and CdSe/ZnS) into chitosan-based biopolymer particles were investigated.
30775747	1	32	theme	types	251:255	arg1	nanocrystals					215:226	green-emitting semiconductor nanocrystals	186:226	green-emitting semiconductor nanocrystals (NCs) of two different types (thiol-capped CdTe and CdSe/ZnS)	186:288	The photoluminescence (PL) properties of composites obtained by embedding green-emitting semiconductor nanocrystals (NCs) of two different types (thiol-capped CdTe and CdSe/ZnS) into chitosan-based biopolymer particles were investigated.
30775747	2	33	theme	chitosan	499:506	arg1	chains					508:513	positively charged chitosan chains	480:513	positively charged chitosan chains	480:513	The synthesis of self-assembled particles from oppositely charged polysaccharides involved a preliminary electrostatic binding of positively charged chitosan chains by negatively charged functional groups of NC stabilizing ligands.
30775747	0	34	theme	chitosan-based	47:60	arg1	composites					62:71	self-assembled chitosan-based composites	32:71	self-assembled chitosan-based composites	32:71	Photoluminescence properties of self-assembled chitosan-based composites containing semiconductor nanocrystals.
30775747	2	35	theme	stabilizing	561:571	arg1	ligands					573:579	NC stabilizing ligands	558:579	NC stabilizing ligands	558:579	The synthesis of self-assembled particles from oppositely charged polysaccharides involved a preliminary electrostatic binding of positively charged chitosan chains by negatively charged functional groups of NC stabilizing ligands.
30775747	2	36	theme	charged	529:535	arg1	groups					548:553	negatively charged functional groups	518:553	negatively charged functional groups of NC stabilizing ligands	518:579	The synthesis of self-assembled particles from oppositely charged polysaccharides involved a preliminary electrostatic binding of positively charged chitosan chains by negatively charged functional groups of NC stabilizing ligands.
30775747	2	36	theme	charged	529:535	arg1	ligands					573:579	NC stabilizing ligands	558:579	NC stabilizing ligands	558:579	The synthesis of self-assembled particles from oppositely charged polysaccharides involved a preliminary electrostatic binding of positively charged chitosan chains by negatively charged functional groups of NC stabilizing ligands.
30775747	2	37	theme	charged	491:497	arg1	chains					508:513	positively charged chitosan chains	480:513	positively charged chitosan chains	480:513	The synthesis of self-assembled particles from oppositely charged polysaccharides involved a preliminary electrostatic binding of positively charged chitosan chains by negatively charged functional groups of NC stabilizing ligands.
30775747	0	38	theme	semiconductor	84:96	arg1	nanocrystals					98:109	semiconductor nanocrystals	84:109	semiconductor nanocrystals	84:109	Photoluminescence properties of self-assembled chitosan-based composites containing semiconductor nanocrystals.
30775747	2	39	theme	charged	408:414	arg1	polysaccharides					416:430	oppositely charged polysaccharides	397:430	oppositely charged polysaccharides	397:430	The synthesis of self-assembled particles from oppositely charged polysaccharides involved a preliminary electrostatic binding of positively charged chitosan chains by negatively charged functional groups of NC stabilizing ligands.
30775747	1	40	theme	composites	153:162	arg1	properties					139:148	The photoluminescence (PL) properties	112:148	The photoluminescence (PL) properties of composites obtained by embedding green-emitting semiconductor nanocrystals (NCs) of two different types (thiol-capped CdTe and CdSe/ZnS) into chitosan-based biopolymer particles	112:329	The photoluminescence (PL) properties of composites obtained by embedding green-emitting semiconductor nanocrystals (NCs) of two different types (thiol-capped CdTe and CdSe/ZnS) into chitosan-based biopolymer particles were investigated.
30775747	1	41	dep	types	251:255	arg1	CdSe/ZnS					280:287	CdSe/ZnS	280:287	CdSe/ZnS	280:287	The photoluminescence (PL) properties of composites obtained by embedding green-emitting semiconductor nanocrystals (NCs) of two different types (thiol-capped CdTe and CdSe/ZnS) into chitosan-based biopolymer particles were investigated.
30775747	1	41	dep	types	251:255	arg1	types					251:255	two different types	237:255	two different types (thiol-capped CdTe and CdSe/ZnS)	237:288	The photoluminescence (PL) properties of composites obtained by embedding green-emitting semiconductor nanocrystals (NCs) of two different types (thiol-capped CdTe and CdSe/ZnS) into chitosan-based biopolymer particles were investigated.
30775747	1	41	dep	types	251:255	arg1	CdTe					271:274	thiol-capped CdTe	258:274	thiol-capped CdTe	258:274	The photoluminescence (PL) properties of composites obtained by embedding green-emitting semiconductor nanocrystals (NCs) of two different types (thiol-capped CdTe and CdSe/ZnS) into chitosan-based biopolymer particles were investigated.
30775747	4	42	theme	stability	756:764	arg1	terms					733:737	terms	733:737	terms of the colloidal stability of the particles and changes in energy gap of NCs	733:814	The PL properties were mainly discussed in terms of the colloidal stability of the particles and changes in energy gap of NCs.
30775747	4	43	theme	energy	798:803	arg1	gap					805:807	energy gap	798:807	energy gap of NCs	798:814	The PL properties were mainly discussed in terms of the colloidal stability of the particles and changes in energy gap of NCs.
30775747	1	44	theme	thiol-capped	258:269	arg1	types					251:255	two different types	237:255	two different types (thiol-capped CdTe and CdSe/ZnS)	237:288	The photoluminescence (PL) properties of composites obtained by embedding green-emitting semiconductor nanocrystals (NCs) of two different types (thiol-capped CdTe and CdSe/ZnS) into chitosan-based biopolymer particles were investigated.
30775747	1	44	theme	thiol-capped	258:269	arg1	CdTe					271:274	thiol-capped CdTe	258:274	thiol-capped CdTe	258:274	The photoluminescence (PL) properties of composites obtained by embedding green-emitting semiconductor nanocrystals (NCs) of two different types (thiol-capped CdTe and CdSe/ZnS) into chitosan-based biopolymer particles were investigated.
30775747	5	45	with	composites	855:864	arg1	NCs					871:873	NCs	871:873	NCs randomly distributed within a biopolymer particle	871:923	Generally, the obtained biocompatible composites with NCs randomly distributed within a biopolymer particle demonstrated a higher PL resistance to the solution acidity that expands the applicability range of thiol-capped NCs.
30775747	2	46	theme	particles	382:390	arg1	synthesis					354:362	The synthesis	350:362	The synthesis of self-assembled particles from oppositely charged polysaccharides	350:430	The synthesis of self-assembled particles from oppositely charged polysaccharides involved a preliminary electrostatic binding of positively charged chitosan chains by negatively charged functional groups of NC stabilizing ligands.
30775747	4	47	from	changes	787:793	arg1	gap					805:807	energy gap	798:807	energy gap of NCs	798:814	The PL properties were mainly discussed in terms of the colloidal stability of the particles and changes in energy gap of NCs.
30775747	5	48	theme	applicability	1002:1014	arg1	range					1016:1020	the applicability range	998:1020	the applicability range of thiol-capped NCs	998:1040	Generally, the obtained biocompatible composites with NCs randomly distributed within a biopolymer particle demonstrated a higher PL resistance to the solution acidity that expands the applicability range of thiol-capped NCs.
30775747	5	49	theme	solution	968:975	arg1	acidity					977:983	the solution acidity	964:983	the solution acidity that expands the applicability range of thiol-capped NCs	964:1040	Generally, the obtained biocompatible composites with NCs randomly distributed within a biopolymer particle demonstrated a higher PL resistance to the solution acidity that expands the applicability range of thiol-capped NCs.
30775747	2	50	theme	preliminary	443:453	arg1	binding					469:475	a preliminary electrostatic binding	441:475	a preliminary electrostatic binding of positively charged chitosan chains by negatively charged functional groups of NC stabilizing ligands	441:579	The synthesis of self-assembled particles from oppositely charged polysaccharides involved a preliminary electrostatic binding of positively charged chitosan chains by negatively charged functional groups of NC stabilizing ligands.
30775747	2	51	theme	NC	558:559	arg1	ligands					573:579	NC stabilizing ligands	558:579	NC stabilizing ligands	558:579	The synthesis of self-assembled particles from oppositely charged polysaccharides involved a preliminary electrostatic binding of positively charged chitosan chains by negatively charged functional groups of NC stabilizing ligands.
30775747	1	52	theme	green-emitting	186:199	arg1	NCs					229:231	NCs	229:231	NCs	229:231	The photoluminescence (PL) properties of composites obtained by embedding green-emitting semiconductor nanocrystals (NCs) of two different types (thiol-capped CdTe and CdSe/ZnS) into chitosan-based biopolymer particles were investigated.
30775747	1	52	theme	green-emitting	186:199	arg1	nanocrystals					215:226	green-emitting semiconductor nanocrystals	186:226	green-emitting semiconductor nanocrystals (NCs) of two different types (thiol-capped CdTe and CdSe/ZnS)	186:288	The photoluminescence (PL) properties of composites obtained by embedding green-emitting semiconductor nanocrystals (NCs) of two different types (thiol-capped CdTe and CdSe/ZnS) into chitosan-based biopolymer particles were investigated.
30775747	2	53	from	synthesis	354:362	arg1	polysaccharides					416:430	oppositely charged polysaccharides	397:430	oppositely charged polysaccharides	397:430	The synthesis of self-assembled particles from oppositely charged polysaccharides involved a preliminary electrostatic binding of positively charged chitosan chains by negatively charged functional groups of NC stabilizing ligands.
30775747	4	54	theme	PL	694:695	arg1	properties					697:706	The PL properties	690:706	The PL properties	690:706	The PL properties were mainly discussed in terms of the colloidal stability of the particles and changes in energy gap of NCs.
31733247	0	0	theme	colonic	118:124	arg1	infections					136:145	colonic bacterial infections	118:145	colonic bacterial infections	118:145	Carboxymethylcellulose/layered double hydroxides bio-nanocomposite hydrogel: A controlled amoxicillin nanocarrier for colonic bacterial infections treatment.
31733247	5	1	theme	antibiotic	724:733	arg1	drug					735:738	the antibiotic drug	720:738	the antibiotic drug	720:738	Amoxicillin (AMX) as a model of the antibiotic drug was selected to study the ability of the prepared systems as an oral drug delivery vehicle.
31733247	5	2	theme	prepared	781:788	arg1	systems					790:796	the prepared systems	777:796	the prepared systems	777:796	Amoxicillin (AMX) as a model of the antibiotic drug was selected to study the ability of the prepared systems as an oral drug delivery vehicle.
31733247	8	3	theme	AMX	1219:1221	arg1	delivery					1228:1235	AMX oral delivery	1219:1235	AMX oral delivery	1219:1235	Consistent with the obtained results, the prepared hydrogel beads could be potentially proposed as an efficient safe drug carrier for AMX oral delivery.
31733247	7	4	theme	MTT	994:996	arg1	assay					998:1002	MTT assay	994:1002	MTT assay	994:1002	MTT assay approved the safety of this bio-nanocomposite hydrogel bead against HUVEC cells.
31733247	5	5	theme	drug	735:738	arg1	model					711:715	a model	709:715	a model of the antibiotic drug	709:738	Amoxicillin (AMX) as a model of the antibiotic drug was selected to study the ability of the prepared systems as an oral drug delivery vehicle.
31733247	2	6	from	presence	452:459	arg1	matrix					477:482	the hydrogel matrix	464:482	the hydrogel matrix	464:482	EDX spectroscopy was used to confirm the successful composition of LDH(Cu/Al) with CMC and its presence in the hydrogel matrix.
31733247	2	7	used	used	378:381	arg2	spectroscopy					361:372	EDX spectroscopy	357:372	EDX spectroscopy	357:372	EDX spectroscopy was used to confirm the successful composition of LDH(Cu/Al) with CMC and its presence in the hydrogel matrix.
31733247	3	8	theme	Cu/Al	506:510	arg1	bead					540:543	The prepared CMC/LDH(Cu/Al) bio-nanocomposite hydrogel bead	485:543	The prepared CMC/LDH(Cu/Al) bio-nanocomposite hydrogel bead	485:543	The prepared CMC/LDH(Cu/Al) bio-nanocomposite hydrogel bead characterized by XRD, FT-IR, and SEM analysis.
31733247	8	9	theme	safe	1197:1200	arg1	carrier					1207:1213	an efficient safe drug carrier	1184:1213	an efficient safe drug carrier for AMX oral delivery	1184:1235	Consistent with the obtained results, the prepared hydrogel beads could be potentially proposed as an efficient safe drug carrier for AMX oral delivery.
31733247	8	9	theme	safe	1197:1200	arg1	beads					1145:1149	the prepared hydrogel beads	1123:1149	the prepared hydrogel beads	1123:1149	Consistent with the obtained results, the prepared hydrogel beads could be potentially proposed as an efficient safe drug carrier for AMX oral delivery.
31733247	8	10	theme	efficient	1187:1195	arg1	carrier					1207:1213	an efficient safe drug carrier	1184:1213	an efficient safe drug carrier for AMX oral delivery	1184:1235	Consistent with the obtained results, the prepared hydrogel beads could be potentially proposed as an efficient safe drug carrier for AMX oral delivery.
31733247	8	10	theme	efficient	1187:1195	arg1	beads					1145:1149	the prepared hydrogel beads	1123:1149	the prepared hydrogel beads	1123:1149	Consistent with the obtained results, the prepared hydrogel beads could be potentially proposed as an efficient safe drug carrier for AMX oral delivery.
31733247	6	11	theme	prepared	965:972	arg1	bio-nanocomposites					974:991	the other prepared bio-nanocomposites	955:991	the other prepared bio-nanocomposites	955:991	The obtained results showed that the CMC/LDH(Cu/Al 7.5) bio-nanocomposite hydrogel bead has a good performance compared to the other prepared bio-nanocomposites.
31733247	1	12	dep	prepared	330:337	arg1	Cu/Al					348:352	Cu/Al	348:352	Cu/Al	348:352	In this work, a pH-sensitive carboxymethylcellulose based bio-nanocomposite hydrogel beads with different content of layered double hydroxides (LDHs) as a nanoparticle was prepared (CMC/LDH(Cu/Al)).
31733247	1	12	dep	prepared	330:337	arg1	CMC/LDH					340:346	CMC/LDH	340:346	CMC/LDH(Cu/Al)	340:353	In this work, a pH-sensitive carboxymethylcellulose based bio-nanocomposite hydrogel beads with different content of layered double hydroxides (LDHs) as a nanoparticle was prepared (CMC/LDH(Cu/Al)).
31733247	6	13	theme	good	926:929	arg1	performance					931:941	a good performance	924:941	a good performance	924:941	The obtained results showed that the CMC/LDH(Cu/Al 7.5) bio-nanocomposite hydrogel bead has a good performance compared to the other prepared bio-nanocomposites.
31733247	0	14	theme	bacterial	126:134	arg1	infections					136:145	colonic bacterial infections	118:145	colonic bacterial infections	118:145	Carboxymethylcellulose/layered double hydroxides bio-nanocomposite hydrogel: A controlled amoxicillin nanocarrier for colonic bacterial infections treatment.
31733247	7	15	theme	HUVEC	1072:1076	arg1	cells					1078:1082	HUVEC cells	1072:1082	HUVEC cells	1072:1082	MTT assay approved the safety of this bio-nanocomposite hydrogel bead against HUVEC cells.
31733247	5	16	theme	oral	804:807	arg1	ability					766:772	the ability	762:772	the ability of the prepared systems	762:796	Amoxicillin (AMX) as a model of the antibiotic drug was selected to study the ability of the prepared systems as an oral drug delivery vehicle.
31733247	5	16	theme	oral	804:807	arg1	vehicle					823:829	an oral drug delivery vehicle	801:829	an oral drug delivery vehicle	801:829	Amoxicillin (AMX) as a model of the antibiotic drug was selected to study the ability of the prepared systems as an oral drug delivery vehicle.
31733247	8	17	theme	prepared	1127:1134	arg1	carrier					1207:1213	an efficient safe drug carrier	1184:1213	an efficient safe drug carrier for AMX oral delivery	1184:1235	Consistent with the obtained results, the prepared hydrogel beads could be potentially proposed as an efficient safe drug carrier for AMX oral delivery.
31733247	8	17	theme	prepared	1127:1134	arg1	beads					1145:1149	the prepared hydrogel beads	1123:1149	the prepared hydrogel beads	1123:1149	Consistent with the obtained results, the prepared hydrogel beads could be potentially proposed as an efficient safe drug carrier for AMX oral delivery.
31733247	6	18	theme	obtained	836:843	arg1	results					845:851	The obtained results	832:851	The obtained results	832:851	The obtained results showed that the CMC/LDH(Cu/Al 7.5) bio-nanocomposite hydrogel bead has a good performance compared to the other prepared bio-nanocomposites.
31733247	7	19	theme	hydrogel	1050:1057	arg1	bead					1059:1062	this bio-nanocomposite hydrogel bead	1027:1062	this bio-nanocomposite hydrogel bead	1027:1062	MTT assay approved the safety of this bio-nanocomposite hydrogel bead against HUVEC cells.
31733247	8	20	with	Consistent	1085:1094	arg1	results					1114:1120	the obtained results	1101:1120	the obtained results	1101:1120	Consistent with the obtained results, the prepared hydrogel beads could be potentially proposed as an efficient safe drug carrier for AMX oral delivery.
31733247	3	21	theme	hydrogel	531:538	arg1	bead					540:543	The prepared CMC/LDH(Cu/Al) bio-nanocomposite hydrogel bead	485:543	The prepared CMC/LDH(Cu/Al) bio-nanocomposite hydrogel bead	485:543	The prepared CMC/LDH(Cu/Al) bio-nanocomposite hydrogel bead characterized by XRD, FT-IR, and SEM analysis.
31733247	5	22	theme	delivery	814:821	arg1	ability					766:772	the ability	762:772	the ability of the prepared systems	762:796	Amoxicillin (AMX) as a model of the antibiotic drug was selected to study the ability of the prepared systems as an oral drug delivery vehicle.
31733247	5	22	theme	delivery	814:821	arg1	vehicle					823:829	an oral drug delivery vehicle	801:829	an oral drug delivery vehicle	801:829	Amoxicillin (AMX) as a model of the antibiotic drug was selected to study the ability of the prepared systems as an oral drug delivery vehicle.
31733247	1	23	theme	bio-nanocomposite	216:232	arg1	beads					243:247	bio-nanocomposite hydrogel beads	216:247	bio-nanocomposite hydrogel beads with different content of layered double hydroxides (LDHs)	216:306	In this work, a pH-sensitive carboxymethylcellulose based bio-nanocomposite hydrogel beads with different content of layered double hydroxides (LDHs) as a nanoparticle was prepared (CMC/LDH(Cu/Al)).
31733247	1	23	theme	bio-nanocomposite	216:232	arg1	nanoparticle					313:324	a nanoparticle	311:324	a nanoparticle	311:324	In this work, a pH-sensitive carboxymethylcellulose based bio-nanocomposite hydrogel beads with different content of layered double hydroxides (LDHs) as a nanoparticle was prepared (CMC/LDH(Cu/Al)).
31733247	2	24	from	matrix	477:482	arg1	composition					409:419	the successful composition	394:419	the successful composition of LDH(Cu/Al) with CMC and its presence in the hydrogel matrix	394:482	EDX spectroscopy was used to confirm the successful composition of LDH(Cu/Al) with CMC and its presence in the hydrogel matrix.
31733247	7	25	theme	bead	1059:1062	arg1	safety					1017:1022	the safety	1013:1022	the safety of this bio-nanocomposite hydrogel bead against HUVEC cells	1013:1082	MTT assay approved the safety of this bio-nanocomposite hydrogel bead against HUVEC cells.
31733247	6	26	theme	other	959:963	arg1	bio-nanocomposites					974:991	the other prepared bio-nanocomposites	955:991	the other prepared bio-nanocomposites	955:991	The obtained results showed that the CMC/LDH(Cu/Al 7.5) bio-nanocomposite hydrogel bead has a good performance compared to the other prepared bio-nanocomposites.
31733247	4	27	theme	swelling	596:603	arg1	results					605:611	The swelling results	592:611	The swelling results	592:611	The swelling results showed the pH-sensitive properties for all of the prepared CMC/LDH(Cu/Al).
31733247	1	28	theme	hydrogel	234:241	arg1	beads					243:247	bio-nanocomposite hydrogel beads	216:247	bio-nanocomposite hydrogel beads with different content of layered double hydroxides (LDHs)	216:306	In this work, a pH-sensitive carboxymethylcellulose based bio-nanocomposite hydrogel beads with different content of layered double hydroxides (LDHs) as a nanoparticle was prepared (CMC/LDH(Cu/Al)).
31733247	1	28	theme	hydrogel	234:241	arg1	nanoparticle					313:324	a nanoparticle	311:324	a nanoparticle	311:324	In this work, a pH-sensitive carboxymethylcellulose based bio-nanocomposite hydrogel beads with different content of layered double hydroxides (LDHs) as a nanoparticle was prepared (CMC/LDH(Cu/Al)).
31733247	2	29	theme	presence	452:459	arg1	composition					409:419	the successful composition	394:419	the successful composition of LDH(Cu/Al) with CMC and its presence in the hydrogel matrix	394:482	EDX spectroscopy was used to confirm the successful composition of LDH(Cu/Al) with CMC and its presence in the hydrogel matrix.
31733247	3	30	theme	bio-nanocomposite	513:529	arg1	bead					540:543	The prepared CMC/LDH(Cu/Al) bio-nanocomposite hydrogel bead	485:543	The prepared CMC/LDH(Cu/Al) bio-nanocomposite hydrogel bead	485:543	The prepared CMC/LDH(Cu/Al) bio-nanocomposite hydrogel bead characterized by XRD, FT-IR, and SEM analysis.
31733247	2	31	with	LDH	424:426	arg1	CMC					440:442	CMC	440:442	CMC	440:442	EDX spectroscopy was used to confirm the successful composition of LDH(Cu/Al) with CMC and its presence in the hydrogel matrix.
31733247	4	32	theme	prepared	663:670	arg1	Cu/Al					680:684	Cu/Al	680:684	Cu/Al	680:684	The swelling results showed the pH-sensitive properties for all of the prepared CMC/LDH(Cu/Al).
31733247	4	32	theme	prepared	663:670	arg1	CMC/LDH					672:678	the prepared CMC/LDH	659:678	the prepared CMC/LDH(Cu/Al)	659:685	The swelling results showed the pH-sensitive properties for all of the prepared CMC/LDH(Cu/Al).
31733247	0	33	theme	Carboxymethylcellulose/layered	0:29	arg1	hydroxides					38:47	Carboxymethylcellulose/layered double hydroxides	0:47	Carboxymethylcellulose/layered double hydroxides	0:47	Carboxymethylcellulose/layered double hydroxides bio-nanocomposite hydrogel: A controlled amoxicillin nanocarrier for colonic bacterial infections treatment.
31733247	7	34	theme	bio-nanocomposite	1032:1048	arg1	bead					1059:1062	this bio-nanocomposite hydrogel bead	1027:1062	this bio-nanocomposite hydrogel bead	1027:1062	MTT assay approved the safety of this bio-nanocomposite hydrogel bead against HUVEC cells.
31733247	8	35	theme	obtained	1105:1112	arg1	results					1114:1120	the obtained results	1101:1120	the obtained results	1101:1120	Consistent with the obtained results, the prepared hydrogel beads could be potentially proposed as an efficient safe drug carrier for AMX oral delivery.
31733247	2	36	theme	successful	398:407	arg1	composition					409:419	the successful composition	394:419	the successful composition of LDH(Cu/Al) with CMC and its presence in the hydrogel matrix	394:482	EDX spectroscopy was used to confirm the successful composition of LDH(Cu/Al) with CMC and its presence in the hydrogel matrix.
31733247	3	37	theme	SEM	578:580	arg1	analysis					582:589	SEM analysis	578:589	SEM analysis	578:589	The prepared CMC/LDH(Cu/Al) bio-nanocomposite hydrogel bead characterized by XRD, FT-IR, and SEM analysis.
31733247	0	38	dep	nanocarrier	102:112	arg1	treatment					147:155	treatment	147:155	hydrogel: A controlled amoxicillin nanocarrier for colonic bacterial infections treatment	67:155	Carboxymethylcellulose/layered double hydroxides bio-nanocomposite hydrogel: A controlled amoxicillin nanocarrier for colonic bacterial infections treatment.
31733247	1	39	theme	different	254:262	arg1	content					264:270	different content	254:270	different content of layered double hydroxides (LDHs)	254:306	In this work, a pH-sensitive carboxymethylcellulose based bio-nanocomposite hydrogel beads with different content of layered double hydroxides (LDHs) as a nanoparticle was prepared (CMC/LDH(Cu/Al)).
31733247	6	40	theme	hydrogel	906:913	arg1	bead					915:918	the CMC/LDH(Cu/Al 7.5) bio-nanocomposite hydrogel bead	865:918	the CMC/LDH(Cu/Al 7.5) bio-nanocomposite hydrogel bead	865:918	The obtained results showed that the CMC/LDH(Cu/Al 7.5) bio-nanocomposite hydrogel bead has a good performance compared to the other prepared bio-nanocomposites.
31733247	2	41	with	presence	452:459	arg1	CMC					440:442	CMC	440:442	CMC	440:442	EDX spectroscopy was used to confirm the successful composition of LDH(Cu/Al) with CMC and its presence in the hydrogel matrix.
31733247	5	42	theme	systems	790:796	arg1	ability					766:772	the ability	762:772	the ability of the prepared systems	762:796	Amoxicillin (AMX) as a model of the antibiotic drug was selected to study the ability of the prepared systems as an oral drug delivery vehicle.
31733247	5	42	theme	systems	790:796	arg1	vehicle					823:829	an oral drug delivery vehicle	801:829	an oral drug delivery vehicle	801:829	Amoxicillin (AMX) as a model of the antibiotic drug was selected to study the ability of the prepared systems as an oral drug delivery vehicle.
31733247	6	43	theme	bio-nanocomposite	888:904	arg1	bead					915:918	the CMC/LDH(Cu/Al 7.5) bio-nanocomposite hydrogel bead	865:918	the CMC/LDH(Cu/Al 7.5) bio-nanocomposite hydrogel bead	865:918	The obtained results showed that the CMC/LDH(Cu/Al 7.5) bio-nanocomposite hydrogel bead has a good performance compared to the other prepared bio-nanocomposites.
31733247	8	44	theme	drug	1202:1205	arg1	carrier					1207:1213	an efficient safe drug carrier	1184:1213	an efficient safe drug carrier for AMX oral delivery	1184:1235	Consistent with the obtained results, the prepared hydrogel beads could be potentially proposed as an efficient safe drug carrier for AMX oral delivery.
31733247	8	44	theme	drug	1202:1205	arg1	beads					1145:1149	the prepared hydrogel beads	1123:1149	the prepared hydrogel beads	1123:1149	Consistent with the obtained results, the prepared hydrogel beads could be potentially proposed as an efficient safe drug carrier for AMX oral delivery.
31733247	2	45	theme	LDH	424:426	arg1	composition					409:419	the successful composition	394:419	the successful composition of LDH(Cu/Al) with CMC and its presence in the hydrogel matrix	394:482	EDX spectroscopy was used to confirm the successful composition of LDH(Cu/Al) with CMC and its presence in the hydrogel matrix.
31733247	2	46	theme	hydrogel	468:475	arg1	matrix					477:482	the hydrogel matrix	464:482	the hydrogel matrix	464:482	EDX spectroscopy was used to confirm the successful composition of LDH(Cu/Al) with CMC and its presence in the hydrogel matrix.
31733247	2	47	from	composition	409:419	arg1	matrix					477:482	the hydrogel matrix	464:482	the hydrogel matrix	464:482	EDX spectroscopy was used to confirm the successful composition of LDH(Cu/Al) with CMC and its presence in the hydrogel matrix.
31733247	1	48	theme	layered	275:281	arg1	LDHs					302:305	LDHs	302:305	LDHs	302:305	In this work, a pH-sensitive carboxymethylcellulose based bio-nanocomposite hydrogel beads with different content of layered double hydroxides (LDHs) as a nanoparticle was prepared (CMC/LDH(Cu/Al)).
31733247	1	48	theme	layered	275:281	arg1	hydroxides					290:299	layered double hydroxides	275:299	layered double hydroxides (LDHs)	275:306	In this work, a pH-sensitive carboxymethylcellulose based bio-nanocomposite hydrogel beads with different content of layered double hydroxides (LDHs) as a nanoparticle was prepared (CMC/LDH(Cu/Al)).
31733247	0	49	theme	controlled	79:88	arg1	nanocarrier					102:112	A controlled amoxicillin nanocarrier	77:112	hydrogel: A controlled amoxicillin nanocarrier for colonic bacterial infections treatment	67:155	Carboxymethylcellulose/layered double hydroxides bio-nanocomposite hydrogel: A controlled amoxicillin nanocarrier for colonic bacterial infections treatment.
31733247	1	50	with	beads	243:247	arg1	content					264:270	different content	254:270	different content of layered double hydroxides (LDHs)	254:306	In this work, a pH-sensitive carboxymethylcellulose based bio-nanocomposite hydrogel beads with different content of layered double hydroxides (LDHs) as a nanoparticle was prepared (CMC/LDH(Cu/Al)).
31733247	3	51	theme	prepared	489:496	arg1	bead					540:543	The prepared CMC/LDH(Cu/Al) bio-nanocomposite hydrogel bead	485:543	The prepared CMC/LDH(Cu/Al) bio-nanocomposite hydrogel bead	485:543	The prepared CMC/LDH(Cu/Al) bio-nanocomposite hydrogel bead characterized by XRD, FT-IR, and SEM analysis.
31733247	6	52	theme	Cu/Al	877:881	arg1	bead					915:918	the CMC/LDH(Cu/Al 7.5) bio-nanocomposite hydrogel bead	865:918	the CMC/LDH(Cu/Al 7.5) bio-nanocomposite hydrogel bead	865:918	The obtained results showed that the CMC/LDH(Cu/Al 7.5) bio-nanocomposite hydrogel bead has a good performance compared to the other prepared bio-nanocomposites.
31733247	0	53	dep	hydrogel	67:74	arg1	nanocarrier					102:112	A controlled amoxicillin nanocarrier	77:112	hydrogel: A controlled amoxicillin nanocarrier for colonic bacterial infections treatment	67:155	Carboxymethylcellulose/layered double hydroxides bio-nanocomposite hydrogel: A controlled amoxicillin nanocarrier for colonic bacterial infections treatment.
31733247	2	54	from	LDH	424:426	arg1	matrix					477:482	the hydrogel matrix	464:482	the hydrogel matrix	464:482	EDX spectroscopy was used to confirm the successful composition of LDH(Cu/Al) with CMC and its presence in the hydrogel matrix.
31733247	3	55	theme	CMC/LDH	498:504	arg1	bead					540:543	The prepared CMC/LDH(Cu/Al) bio-nanocomposite hydrogel bead	485:543	The prepared CMC/LDH(Cu/Al) bio-nanocomposite hydrogel bead	485:543	The prepared CMC/LDH(Cu/Al) bio-nanocomposite hydrogel bead characterized by XRD, FT-IR, and SEM analysis.
31733247	2	56	theme	EDX	357:359	arg1	spectroscopy					361:372	EDX spectroscopy	357:372	EDX spectroscopy	357:372	EDX spectroscopy was used to confirm the successful composition of LDH(Cu/Al) with CMC and its presence in the hydrogel matrix.
31733247	1	57	theme	double	283:288	arg1	LDHs					302:305	LDHs	302:305	LDHs	302:305	In this work, a pH-sensitive carboxymethylcellulose based bio-nanocomposite hydrogel beads with different content of layered double hydroxides (LDHs) as a nanoparticle was prepared (CMC/LDH(Cu/Al)).
31733247	1	57	theme	double	283:288	arg1	hydroxides					290:299	layered double hydroxides	275:299	layered double hydroxides (LDHs)	275:306	In this work, a pH-sensitive carboxymethylcellulose based bio-nanocomposite hydrogel beads with different content of layered double hydroxides (LDHs) as a nanoparticle was prepared (CMC/LDH(Cu/Al)).
31733247	8	58	theme	hydrogel	1136:1143	arg1	carrier					1207:1213	an efficient safe drug carrier	1184:1213	an efficient safe drug carrier for AMX oral delivery	1184:1235	Consistent with the obtained results, the prepared hydrogel beads could be potentially proposed as an efficient safe drug carrier for AMX oral delivery.
31733247	8	58	theme	hydrogel	1136:1143	arg1	beads					1145:1149	the prepared hydrogel beads	1123:1149	the prepared hydrogel beads	1123:1149	Consistent with the obtained results, the prepared hydrogel beads could be potentially proposed as an efficient safe drug carrier for AMX oral delivery.
31733247	1	59	theme	pH-sensitive	174:185	arg1	carboxymethylcellulose					187:208	a pH-sensitive carboxymethylcellulose	172:208	a pH-sensitive carboxymethylcellulose based bio-nanocomposite hydrogel beads with different content of layered double hydroxides (LDHs) as a nanoparticle	172:324	In this work, a pH-sensitive carboxymethylcellulose based bio-nanocomposite hydrogel beads with different content of layered double hydroxides (LDHs) as a nanoparticle was prepared (CMC/LDH(Cu/Al)).
31733247	1	60	theme	hydroxides	290:299	arg1	content					264:270	different content	254:270	different content of layered double hydroxides (LDHs)	254:306	In this work, a pH-sensitive carboxymethylcellulose based bio-nanocomposite hydrogel beads with different content of layered double hydroxides (LDHs) as a nanoparticle was prepared (CMC/LDH(Cu/Al)).
31733247	8	61	theme	oral	1223:1226	arg1	delivery					1228:1235	AMX oral delivery	1219:1235	AMX oral delivery	1219:1235	Consistent with the obtained results, the prepared hydrogel beads could be potentially proposed as an efficient safe drug carrier for AMX oral delivery.
31733247	4	62	theme	pH-sensitive	624:635	arg1	properties					637:646	the pH-sensitive properties	620:646	the pH-sensitive properties for all of the prepared CMC/LDH(Cu/Al)	620:685	The swelling results showed the pH-sensitive properties for all of the prepared CMC/LDH(Cu/Al).
31733247	6	63	contain	has	920:922	arg1	bead					915:918	the CMC/LDH(Cu/Al 7.5) bio-nanocomposite hydrogel bead	865:918	the CMC/LDH(Cu/Al 7.5) bio-nanocomposite hydrogel bead	865:918	The obtained results showed that the CMC/LDH(Cu/Al 7.5) bio-nanocomposite hydrogel bead has a good performance compared to the other prepared bio-nanocomposites.
31733247	6	63	contain	has	920:922	arg2	performance					931:941	a good performance	924:941	a good performance	924:941	The obtained results showed that the CMC/LDH(Cu/Al 7.5) bio-nanocomposite hydrogel bead has a good performance compared to the other prepared bio-nanocomposites.
31733247	5	64	theme	drug	809:812	arg1	ability					766:772	the ability	762:772	the ability of the prepared systems	762:796	Amoxicillin (AMX) as a model of the antibiotic drug was selected to study the ability of the prepared systems as an oral drug delivery vehicle.
31733247	5	64	theme	drug	809:812	arg1	vehicle					823:829	an oral drug delivery vehicle	801:829	an oral drug delivery vehicle	801:829	Amoxicillin (AMX) as a model of the antibiotic drug was selected to study the ability of the prepared systems as an oral drug delivery vehicle.
31733247	0	65	theme	amoxicillin	90:100	arg1	nanocarrier					102:112	A controlled amoxicillin nanocarrier	77:112	hydrogel: A controlled amoxicillin nanocarrier for colonic bacterial infections treatment	67:155	Carboxymethylcellulose/layered double hydroxides bio-nanocomposite hydrogel: A controlled amoxicillin nanocarrier for colonic bacterial infections treatment.
31733247	6	66	theme	CMC/LDH	869:875	arg1	bead					915:918	the CMC/LDH(Cu/Al 7.5) bio-nanocomposite hydrogel bead	865:918	the CMC/LDH(Cu/Al 7.5) bio-nanocomposite hydrogel bead	865:918	The obtained results showed that the CMC/LDH(Cu/Al 7.5) bio-nanocomposite hydrogel bead has a good performance compared to the other prepared bio-nanocomposites.
31733247	0	67	theme	double	31:36	arg1	hydroxides					38:47	Carboxymethylcellulose/layered double hydroxides	0:47	Carboxymethylcellulose/layered double hydroxides	0:47	Carboxymethylcellulose/layered double hydroxides bio-nanocomposite hydrogel: A controlled amoxicillin nanocarrier for colonic bacterial infections treatment.
31233555	7	0	theme	sialoglycans	1136:1147	arg1	recognition					1112:1122	the recognition	1108:1122	the recognition of specific sialoglycans	1108:1147	The findings indicate that the recognition of specific sialoglycans can either enhance or diminish pathogenicity.
31233555	8	1	theme	valve	1405:1409	arg1	tissue					1411:1416	valve tissue	1405:1416	valve tissue 72 hours later	1405:1431	Binding to sialyllactosamine reduces the initial colonization of mechanically-damaged aortic valves, whereas binding to the closely-related trisaccharide sialyl T-antigen promotes higher bacterial densities in valve tissue 72 hours later.
31233555	10	2	theme	modified	1991:1998	arg1	acids					2007:2011	modified sialic acids	1991:2011	modified sialic acids	1991:2011	Importantly, we found that human and rat platelet GPIbα (the major receptor for S. gordonii and S. sanguinis on platelets) display similar O-glycan structures, comprised mainly of a di-sialylated core 2 hexasaccharide, although the rat GPIbα has a more heterogenous composition of modified sialic acids.
31233555	4	3	theme	valve	639:643	arg1	infections					645:654	aortic valve infections	632:654	aortic valve infections caused by some S. gordonii	632:681	Previous studies have shown that sialoglycan binding is clearly important for aortic valve infections caused by some S. gordonii, but this process did not contribute to the virulence of a strain of S. sanguinis.
31233555	3	4	gly	glycoproteins	456:468	arg1	glycoproteins					456:468	mucin-like glycoproteins	445:468	mucin-like glycoproteins	445:468	Both streptococcal species express a serine-rich repeat surface adhesin that mediates attachment to sialylated glycans on mucin-like glycoproteins, but the specific sialoglycan structures recognized can vary from strain to strain.
31233555	8	5	theme	mechanically-damaged	1260:1279	arg1	valves					1288:1293	mechanically-damaged aortic valves	1260:1293	mechanically-damaged aortic valves	1260:1293	Binding to sialyllactosamine reduces the initial colonization of mechanically-damaged aortic valves, whereas binding to the closely-related trisaccharide sialyl T-antigen promotes higher bacterial densities in valve tissue 72 hours later.
31233555	10	6	theme	major	1771:1775	arg1	receptor					1777:1784	the major receptor	1767:1784	the major receptor for S. gordonii and S. sanguinis on platelets	1767:1830	Importantly, we found that human and rat platelet GPIbα (the major receptor for S. gordonii and S. sanguinis on platelets) display similar O-glycan structures, comprised mainly of a di-sialylated core 2 hexasaccharide, although the rat GPIbα has a more heterogenous composition of modified sialic acids.
31233555	4	7	theme	aortic	632:637	arg1	infections					645:654	aortic valve infections	632:654	aortic valve infections caused by some S. gordonii	632:681	Previous studies have shown that sialoglycan binding is clearly important for aortic valve infections caused by some S. gordonii, but this process did not contribute to the virulence of a strain of S. sanguinis.
31233555	0	8	theme	infection	104:112	arg1	severity					80:87	the severity	76:87	the severity of endocardial infection	76:112	Recognition of specific sialoglycan structures by oral streptococci impacts the severity of endocardial infection.
31233555	10	9	theme	O-glycan	1849:1856	arg1	structures					1858:1867	similar O-glycan structures	1841:1867	similar O-glycan structures	1841:1867	Importantly, we found that human and rat platelet GPIbα (the major receptor for S. gordonii and S. sanguinis on platelets) display similar O-glycan structures, comprised mainly of a di-sialylated core 2 hexasaccharide, although the rat GPIbα has a more heterogenous composition of modified sialic acids.
31233555	2	10	theme	endocarditis	309:320	arg1	cause					290:294	a frequent cause	279:294	a frequent cause of infective endocarditis	279:320	Although generally non-pathogenic in the oral environment, they are a frequent cause of infective endocarditis.
31233555	2	10	theme	endocarditis	309:320	arg1	they					270:273	they	270:273	they	270:273	Although generally non-pathogenic in the oral environment, they are a frequent cause of infective endocarditis.
31233555	10	11	dep	GPIbα	1760:1764	arg1	receptor					1777:1784	the major receptor	1767:1784	the major receptor for S. gordonii and S. sanguinis on platelets	1767:1830	Importantly, we found that human and rat platelet GPIbα (the major receptor for S. gordonii and S. sanguinis on platelets) display similar O-glycan structures, comprised mainly of a di-sialylated core 2 hexasaccharide, although the rat GPIbα has a more heterogenous composition of modified sialic acids.
31233555	9	12	theme	streptococci	1490:1501	arg1	proportional					1534:1545	proportional	1534:1545	proportional	1534:1545	A surprising finding was that the initial attachment of streptococci to aortic valves was inversely proportional to the affinity of each strain for platelets, suggesting that binding to platelets circulating in the blood may divert bacteria away from the endocardial surface.
31233555	9	12	theme	streptococci	1490:1501	arg1	attachment					1476:1485	the initial attachment	1464:1485	the initial attachment of streptococci to aortic valves	1464:1518	A surprising finding was that the initial attachment of streptococci to aortic valves was inversely proportional to the affinity of each strain for platelets, suggesting that binding to platelets circulating in the blood may divert bacteria away from the endocardial surface.
31233555	0	13	theme	endocardial	92:102	arg1	infection					104:112	endocardial infection	92:112	endocardial infection	92:112	Recognition of specific sialoglycan structures by oral streptococci impacts the severity of endocardial infection.
31233555	3	14	from	attachment	409:418	arg1	glycoproteins					456:468	mucin-like glycoproteins	445:468	mucin-like glycoproteins	445:468	Both streptococcal species express a serine-rich repeat surface adhesin that mediates attachment to sialylated glycans on mucin-like glycoproteins, but the specific sialoglycan structures recognized can vary from strain to strain.
31233555	7	15	theme	specific	1127:1134	arg1	sialoglycans					1136:1147	specific sialoglycans	1127:1147	specific sialoglycans	1127:1147	The findings indicate that the recognition of specific sialoglycans can either enhance or diminish pathogenicity.
31233555	2	16	theme	infective	299:307	arg1	endocarditis					309:320	infective endocarditis	299:320	infective endocarditis	299:320	Although generally non-pathogenic in the oral environment, they are a frequent cause of infective endocarditis.
31233555	10	17	theme	platelet	1751:1758	arg1	GPIbα					1760:1764	rat platelet GPIbα	1747:1764	rat platelet GPIbα (the major receptor for S. gordonii and S. sanguinis on platelets)	1747:1831	Importantly, we found that human and rat platelet GPIbα (the major receptor for S. gordonii and S. sanguinis on platelets) display similar O-glycan structures, comprised mainly of a di-sialylated core 2 hexasaccharide, although the rat GPIbα has a more heterogenous composition of modified sialic acids.
31233555	3	18	theme	surface	379:385	arg1	adhesin					387:393	a serine-rich repeat surface adhesin	358:393	a serine-rich repeat surface adhesin that mediates attachment to sialylated glycans on mucin-like glycoproteins	358:468	Both streptococcal species express a serine-rich repeat surface adhesin that mediates attachment to sialylated glycans on mucin-like glycoproteins, but the specific sialoglycan structures recognized can vary from strain to strain.
31233555	5	19	theme	structures	839:848	arg1	subsets					816:822	different subsets	806:822	different subsets of sialoglycan structures	806:848	However, these streptococci can bind to different subsets of sialoglycan structures.
31233555	11	20	theme	streptococcal	2048:2060	arg1	interaction					2062:2072	streptococcal interaction	2048:2072	streptococcal interaction with a minor O-glycan on GPIbα	2048:2103	The combined results suggest that streptococcal interaction with a minor O-glycan on GPIbα may be more important than the over-all affinity for GPIbα for pathogenic effects.
31233555	10	21	contain	has	1952:1954	arg1	GPIbα					1946:1950	the rat GPIbα	1938:1950	the rat GPIbα	1938:1950	Importantly, we found that human and rat platelet GPIbα (the major receptor for S. gordonii and S. sanguinis on platelets) display similar O-glycan structures, comprised mainly of a di-sialylated core 2 hexasaccharide, although the rat GPIbα has a more heterogenous composition of modified sialic acids.
31233555	10	21	contain	has	1952:1954	arg2	composition					1976:1986	a more heterogenous composition	1956:1986	a more heterogenous composition of modified sialic acids	1956:2011	Importantly, we found that human and rat platelet GPIbα (the major receptor for S. gordonii and S. sanguinis on platelets) display similar O-glycan structures, comprised mainly of a di-sialylated core 2 hexasaccharide, although the rat GPIbα has a more heterogenous composition of modified sialic acids.
31233555	3	22	theme	specific	479:486	arg1	structures					500:509	the specific sialoglycan structures	475:509	the specific sialoglycan structures recognized	475:520	Both streptococcal species express a serine-rich repeat surface adhesin that mediates attachment to sialylated glycans on mucin-like glycoproteins, but the specific sialoglycan structures recognized can vary from strain to strain.
31233555	2	23	from	non-pathogenic	230:243	arg1	environment					257:267	the oral environment	248:267	the oral environment	248:267	Although generally non-pathogenic in the oral environment, they are a frequent cause of infective endocarditis.
31233555	3	24	gly	sialylated	423:432	arg1	glycans					434:440	sialylated glycans	423:440	sialylated glycans	423:440	Both streptococcal species express a serine-rich repeat surface adhesin that mediates attachment to sialylated glycans on mucin-like glycoproteins, but the specific sialoglycan structures recognized can vary from strain to strain.
31233555	10	25	from	receptor	1777:1784	arg1	platelets					1822:1830	platelets	1822:1830	platelets	1822:1830	Importantly, we found that human and rat platelet GPIbα (the major receptor for S. gordonii and S. sanguinis on platelets) display similar O-glycan structures, comprised mainly of a di-sialylated core 2 hexasaccharide, although the rat GPIbα has a more heterogenous composition of modified sialic acids.
31233555	3	26	theme	sialoglycan	488:498	arg1	structures					500:509	the specific sialoglycan structures	475:509	the specific sialoglycan structures recognized	475:520	Both streptococcal species express a serine-rich repeat surface adhesin that mediates attachment to sialylated glycans on mucin-like glycoproteins, but the specific sialoglycan structures recognized can vary from strain to strain.
31233555	3	27	theme	sialylated	423:432	arg1	glycans					434:440	sialylated glycans	423:440	sialylated glycans	423:440	Both streptococcal species express a serine-rich repeat surface adhesin that mediates attachment to sialylated glycans on mucin-like glycoproteins, but the specific sialoglycan structures recognized can vary from strain to strain.
31233555	10	28	theme	heterogenous	1963:1974	arg1	composition					1976:1986	a more heterogenous composition	1956:1986	a more heterogenous composition of modified sialic acids	1956:2011	Importantly, we found that human and rat platelet GPIbα (the major receptor for S. gordonii and S. sanguinis on platelets) display similar O-glycan structures, comprised mainly of a di-sialylated core 2 hexasaccharide, although the rat GPIbα has a more heterogenous composition of modified sialic acids.
31233555	9	29	attach	attachment	1476:1485	arg2	streptococci					1490:1501	streptococci	1490:1501	streptococci	1490:1501	A surprising finding was that the initial attachment of streptococci to aortic valves was inversely proportional to the affinity of each strain for platelets, suggesting that binding to platelets circulating in the blood may divert bacteria away from the endocardial surface.
31233555	9	29	attach	attachment	1476:1485	arg1	valves					1513:1518	aortic valves	1506:1518	aortic valves	1506:1518	A surprising finding was that the initial attachment of streptococci to aortic valves was inversely proportional to the affinity of each strain for platelets, suggesting that binding to platelets circulating in the blood may divert bacteria away from the endocardial surface.
31233555	3	30	theme	mucin-like	445:454	arg1	glycoproteins					456:468	mucin-like glycoproteins	445:468	mucin-like glycoproteins	445:468	Both streptococcal species express a serine-rich repeat surface adhesin that mediates attachment to sialylated glycans on mucin-like glycoproteins, but the specific sialoglycan structures recognized can vary from strain to strain.
31233555	2	31	from	environment	257:267	arg1	non-pathogenic					230:243	non-pathogenic	230:243	non-pathogenic	230:243	Although generally non-pathogenic in the oral environment, they are a frequent cause of infective endocarditis.
31233555	1	32	theme	surface	202:208	arg1	gordonii					129:136	Streptococcus gordonii	115:136	Streptococcus gordonii	115:136	Streptococcus gordonii and Streptococcus sanguinis are primary colonizers of the tooth surface.
31233555	1	32	theme	surface	202:208	arg1	sanguinis					156:164	Streptococcus sanguinis	142:164	Streptococcus sanguinis	142:164	Streptococcus gordonii and Streptococcus sanguinis are primary colonizers of the tooth surface.
31233555	1	32	theme	surface	202:208	arg1	colonizers					178:187	primary colonizers	170:187	primary colonizers of the tooth surface	170:208	Streptococcus gordonii and Streptococcus sanguinis are primary colonizers of the tooth surface.
31233555	10	33	theme	sialic	2000:2005	arg1	acids					2007:2011	modified sialic acids	1991:2011	modified sialic acids	1991:2011	Importantly, we found that human and rat platelet GPIbα (the major receptor for S. gordonii and S. sanguinis on platelets) display similar O-glycan structures, comprised mainly of a di-sialylated core 2 hexasaccharide, although the rat GPIbα has a more heterogenous composition of modified sialic acids.
31233555	6	34	theme	endocarditis	1067:1078	arg1	model					1058:1062	a rat model	1052:1062	a rat model of endocarditis	1052:1078	Here we generated isogenic strains of S. gordonii that differ only in the type and range of sialoglycan structures to which they adhere and examined whether this rendered them more or less virulent in a rat model of endocarditis.
31233555	0	35	theme	sialoglycan	24:34	arg1	structures					36:45	specific sialoglycan structures	15:45	specific sialoglycan structures	15:45	Recognition of specific sialoglycan structures by oral streptococci impacts the severity of endocardial infection.
31233555	11	36	theme	combined	2018:2025	arg1	results					2027:2033	The combined results	2014:2033	The combined results	2014:2033	The combined results suggest that streptococcal interaction with a minor O-glycan on GPIbα may be more important than the over-all affinity for GPIbα for pathogenic effects.
31233555	9	37	theme	aortic	1506:1511	arg1	valves					1513:1518	aortic valves	1506:1518	aortic valves	1506:1518	A surprising finding was that the initial attachment of streptococci to aortic valves was inversely proportional to the affinity of each strain for platelets, suggesting that binding to platelets circulating in the blood may divert bacteria away from the endocardial surface.
31233555	11	38	theme	minor	2081:2085	arg1	O-glycan					2087:2094	a minor O-glycan	2079:2094	a minor O-glycan on GPIbα	2079:2103	The combined results suggest that streptococcal interaction with a minor O-glycan on GPIbα may be more important than the over-all affinity for GPIbα for pathogenic effects.
31233555	10	39	theme	rat	1747:1749	arg1	GPIbα					1760:1764	rat platelet GPIbα	1747:1764	rat platelet GPIbα (the major receptor for S. gordonii and S. sanguinis on platelets)	1747:1831	Importantly, we found that human and rat platelet GPIbα (the major receptor for S. gordonii and S. sanguinis on platelets) display similar O-glycan structures, comprised mainly of a di-sialylated core 2 hexasaccharide, although the rat GPIbα has a more heterogenous composition of modified sialic acids.
31233555	0	40	theme	specific	15:22	arg1	structures					36:45	specific sialoglycan structures	15:45	specific sialoglycan structures	15:45	Recognition of specific sialoglycan structures by oral streptococci impacts the severity of endocardial infection.
31233555	10	41	theme	acids	2007:2011	arg1	composition					1976:1986	a more heterogenous composition	1956:1986	a more heterogenous composition of modified sialic acids	1956:2011	Importantly, we found that human and rat platelet GPIbα (the major receptor for S. gordonii and S. sanguinis on platelets) display similar O-glycan structures, comprised mainly of a di-sialylated core 2 hexasaccharide, although the rat GPIbα has a more heterogenous composition of modified sialic acids.
31233555	9	42	theme	strain	1571:1576	arg1	affinity					1554:1561	the affinity	1550:1561	the affinity of each strain for platelets	1550:1590	A surprising finding was that the initial attachment of streptococci to aortic valves was inversely proportional to the affinity of each strain for platelets, suggesting that binding to platelets circulating in the blood may divert bacteria away from the endocardial surface.
31233555	5	43	theme	sialoglycan	827:837	arg1	structures					839:848	sialoglycan structures	827:848	sialoglycan structures	827:848	However, these streptococci can bind to different subsets of sialoglycan structures.
31233555	5	44	theme	different	806:814	arg1	subsets					816:822	different subsets	806:822	different subsets of sialoglycan structures	806:848	However, these streptococci can bind to different subsets of sialoglycan structures.
31233555	8	45	theme	sialyl	1349:1354	arg1	T-antigen					1356:1364	the closely-related trisaccharide sialyl T-antigen	1315:1364	the closely-related trisaccharide sialyl T-antigen	1315:1364	Binding to sialyllactosamine reduces the initial colonization of mechanically-damaged aortic valves, whereas binding to the closely-related trisaccharide sialyl T-antigen promotes higher bacterial densities in valve tissue 72 hours later.
31233555	10	46	gly	di-sialylated	1892:1904	arg1	hexasaccharide					1913:1926	a di-sialylated core 2 hexasaccharide	1890:1926	a di-sialylated core 2 hexasaccharide	1890:1926	Importantly, we found that human and rat platelet GPIbα (the major receptor for S. gordonii and S. sanguinis on platelets) display similar O-glycan structures, comprised mainly of a di-sialylated core 2 hexasaccharide, although the rat GPIbα has a more heterogenous composition of modified sialic acids.
31233555	8	47	theme	trisaccharide	1335:1347	arg1	T-antigen					1356:1364	the closely-related trisaccharide sialyl T-antigen	1315:1364	the closely-related trisaccharide sialyl T-antigen	1315:1364	Binding to sialyllactosamine reduces the initial colonization of mechanically-damaged aortic valves, whereas binding to the closely-related trisaccharide sialyl T-antigen promotes higher bacterial densities in valve tissue 72 hours later.
31233555	11	48	theme	over-all	2136:2143	arg1	affinity					2145:2152	the over-all affinity	2132:2152	the over-all affinity for GPIbα for pathogenic effects	2132:2185	The combined results suggest that streptococcal interaction with a minor O-glycan on GPIbα may be more important than the over-all affinity for GPIbα for pathogenic effects.
31233555	6	49	theme	rat	1054:1056	arg1	model					1058:1062	a rat model	1052:1062	a rat model of endocarditis	1052:1078	Here we generated isogenic strains of S. gordonii that differ only in the type and range of sialoglycan structures to which they adhere and examined whether this rendered them more or less virulent in a rat model of endocarditis.
31233555	0	50	theme	structures	36:45	arg1	Recognition					0:10	Recognition	0:10	Recognition of specific sialoglycan structures by oral streptococci	0:66	Recognition of specific sialoglycan structures by oral streptococci impacts the severity of endocardial infection.
31233555	8	51	theme	valves	1288:1293	arg1	colonization					1244:1255	the initial colonization	1232:1255	the initial colonization of mechanically-damaged aortic valves	1232:1293	Binding to sialyllactosamine reduces the initial colonization of mechanically-damaged aortic valves, whereas binding to the closely-related trisaccharide sialyl T-antigen promotes higher bacterial densities in valve tissue 72 hours later.
31233555	11	52	with	interaction	2062:2072	arg1	O-glycan					2087:2094	a minor O-glycan	2079:2094	a minor O-glycan on GPIbα	2079:2103	The combined results suggest that streptococcal interaction with a minor O-glycan on GPIbα may be more important than the over-all affinity for GPIbα for pathogenic effects.
31233555	6	53	theme	gordonii	892:899	arg1	strains					878:884	isogenic strains	869:884	isogenic strains of S. gordonii that differ only in the type and range of sialoglycan structures to which they adhere and examined whether this rendered them more or less virulent in a rat model of endocarditis	869:1078	Here we generated isogenic strains of S. gordonii that differ only in the type and range of sialoglycan structures to which they adhere and examined whether this rendered them more or less virulent in a rat model of endocarditis.
31233555	8	54	theme	initial	1236:1242	arg1	colonization					1244:1255	the initial colonization	1232:1255	the initial colonization of mechanically-damaged aortic valves	1232:1293	Binding to sialyllactosamine reduces the initial colonization of mechanically-damaged aortic valves, whereas binding to the closely-related trisaccharide sialyl T-antigen promotes higher bacterial densities in valve tissue 72 hours later.
31233555	6	55	theme	structures	955:964	arg1	range					934:938	range	934:938	range	934:938	Here we generated isogenic strains of S. gordonii that differ only in the type and range of sialoglycan structures to which they adhere and examined whether this rendered them more or less virulent in a rat model of endocarditis.
31233555	6	55	theme	structures	955:964	arg1	type					925:928	type	925:928	type	925:928	Here we generated isogenic strains of S. gordonii that differ only in the type and range of sialoglycan structures to which they adhere and examined whether this rendered them more or less virulent in a rat model of endocarditis.
31233555	8	56	theme	aortic	1281:1286	arg1	valves					1288:1293	mechanically-damaged aortic valves	1260:1293	mechanically-damaged aortic valves	1260:1293	Binding to sialyllactosamine reduces the initial colonization of mechanically-damaged aortic valves, whereas binding to the closely-related trisaccharide sialyl T-antigen promotes higher bacterial densities in valve tissue 72 hours later.
31233555	3	57	theme	streptococcal	328:340	arg1	species					342:348	Both streptococcal species	323:348	Both streptococcal species	323:348	Both streptococcal species express a serine-rich repeat surface adhesin that mediates attachment to sialylated glycans on mucin-like glycoproteins, but the specific sialoglycan structures recognized can vary from strain to strain.
31233555	10	58	theme	di-sialylated	1892:1904	arg1	hexasaccharide					1913:1926	a di-sialylated core 2 hexasaccharide	1890:1926	a di-sialylated core 2 hexasaccharide	1890:1926	Importantly, we found that human and rat platelet GPIbα (the major receptor for S. gordonii and S. sanguinis on platelets) display similar O-glycan structures, comprised mainly of a di-sialylated core 2 hexasaccharide, although the rat GPIbα has a more heterogenous composition of modified sialic acids.
31233555	2	59	theme	oral	252:255	arg1	environment					257:267	the oral environment	248:267	the oral environment	248:267	Although generally non-pathogenic in the oral environment, they are a frequent cause of infective endocarditis.
31233555	0	60	theme	oral	50:53	arg1	streptococci					55:66	oral streptococci	50:66	oral streptococci	50:66	Recognition of specific sialoglycan structures by oral streptococci impacts the severity of endocardial infection.
31233555	9	61	theme	surprising	1436:1445	arg1	finding					1447:1453	A surprising finding	1434:1453	A surprising finding	1434:1453	A surprising finding was that the initial attachment of streptococci to aortic valves was inversely proportional to the affinity of each strain for platelets, suggesting that binding to platelets circulating in the blood may divert bacteria away from the endocardial surface.
31233555	6	62	theme	sialoglycan	943:953	arg1	structures					955:964	sialoglycan structures	943:964	sialoglycan structures to which they adhere and examined whether this rendered them more or less virulent in a rat model of endocarditis	943:1078	Here we generated isogenic strains of S. gordonii that differ only in the type and range of sialoglycan structures to which they adhere and examined whether this rendered them more or less virulent in a rat model of endocarditis.
31233555	10	63	theme	rat	1942:1944	arg1	GPIbα					1946:1950	the rat GPIbα	1938:1950	the rat GPIbα	1938:1950	Importantly, we found that human and rat platelet GPIbα (the major receptor for S. gordonii and S. sanguinis on platelets) display similar O-glycan structures, comprised mainly of a di-sialylated core 2 hexasaccharide, although the rat GPIbα has a more heterogenous composition of modified sialic acids.
31233555	10	64	theme	similar	1841:1847	arg1	structures					1858:1867	similar O-glycan structures	1841:1867	similar O-glycan structures	1841:1867	Importantly, we found that human and rat platelet GPIbα (the major receptor for S. gordonii and S. sanguinis on platelets) display similar O-glycan structures, comprised mainly of a di-sialylated core 2 hexasaccharide, although the rat GPIbα has a more heterogenous composition of modified sialic acids.
31233555	4	65	theme	strain	742:747	arg1	virulence					727:735	the virulence	723:735	the virulence of a strain of S. sanguinis	723:763	Previous studies have shown that sialoglycan binding is clearly important for aortic valve infections caused by some S. gordonii, but this process did not contribute to the virulence of a strain of S. sanguinis.
31233555	4	66	theme	sialoglycan	587:597	arg1	binding					599:605	sialoglycan binding	587:605	sialoglycan binding	587:605	Previous studies have shown that sialoglycan binding is clearly important for aortic valve infections caused by some S. gordonii, but this process did not contribute to the virulence of a strain of S. sanguinis.
31233555	2	67	theme	frequent	281:288	arg1	cause					290:294	a frequent cause	279:294	a frequent cause of infective endocarditis	279:320	Although generally non-pathogenic in the oral environment, they are a frequent cause of infective endocarditis.
31233555	2	67	theme	frequent	281:288	arg1	they					270:273	they	270:273	they	270:273	Although generally non-pathogenic in the oral environment, they are a frequent cause of infective endocarditis.
31233555	4	68	theme	sanguinis	755:763	arg1	strain					742:747	a strain	740:747	a strain of S. sanguinis	740:763	Previous studies have shown that sialoglycan binding is clearly important for aortic valve infections caused by some S. gordonii, but this process did not contribute to the virulence of a strain of S. sanguinis.
31233555	11	69	from	O-glycan	2087:2094	arg1	GPIbα					2099:2103	GPIbα	2099:2103	GPIbα	2099:2103	The combined results suggest that streptococcal interaction with a minor O-glycan on GPIbα may be more important than the over-all affinity for GPIbα for pathogenic effects.
31233555	6	70	theme	isogenic	869:876	arg1	strains					878:884	isogenic strains	869:884	isogenic strains of S. gordonii that differ only in the type and range of sialoglycan structures to which they adhere and examined whether this rendered them more or less virulent in a rat model of endocarditis	869:1078	Here we generated isogenic strains of S. gordonii that differ only in the type and range of sialoglycan structures to which they adhere and examined whether this rendered them more or less virulent in a rat model of endocarditis.
31233555	9	71	theme	endocardial	1689:1699	arg1	surface					1701:1707	the endocardial surface	1685:1707	the endocardial surface	1685:1707	A surprising finding was that the initial attachment of streptococci to aortic valves was inversely proportional to the affinity of each strain for platelets, suggesting that binding to platelets circulating in the blood may divert bacteria away from the endocardial surface.
31233555	8	72	theme	bacterial	1382:1390	arg1	densities					1392:1400	higher bacterial densities	1375:1400	higher bacterial densities	1375:1400	Binding to sialyllactosamine reduces the initial colonization of mechanically-damaged aortic valves, whereas binding to the closely-related trisaccharide sialyl T-antigen promotes higher bacterial densities in valve tissue 72 hours later.
31233555	8	73	theme	higher	1375:1380	arg1	densities					1392:1400	higher bacterial densities	1375:1400	higher bacterial densities	1375:1400	Binding to sialyllactosamine reduces the initial colonization of mechanically-damaged aortic valves, whereas binding to the closely-related trisaccharide sialyl T-antigen promotes higher bacterial densities in valve tissue 72 hours later.
31233555	3	74	theme	serine-rich	360:370	arg1	adhesin					387:393	a serine-rich repeat surface adhesin	358:393	a serine-rich repeat surface adhesin that mediates attachment to sialylated glycans on mucin-like glycoproteins	358:468	Both streptococcal species express a serine-rich repeat surface adhesin that mediates attachment to sialylated glycans on mucin-like glycoproteins, but the specific sialoglycan structures recognized can vary from strain to strain.
31233555	8	75	theme	closely-related	1319:1333	arg1	T-antigen					1356:1364	the closely-related trisaccharide sialyl T-antigen	1315:1364	the closely-related trisaccharide sialyl T-antigen	1315:1364	Binding to sialyllactosamine reduces the initial colonization of mechanically-damaged aortic valves, whereas binding to the closely-related trisaccharide sialyl T-antigen promotes higher bacterial densities in valve tissue 72 hours later.
31233555	10	76	theme	core	1906:1909	arg1	hexasaccharide					1913:1926	a di-sialylated core 2 hexasaccharide	1890:1926	a di-sialylated core 2 hexasaccharide	1890:1926	Importantly, we found that human and rat platelet GPIbα (the major receptor for S. gordonii and S. sanguinis on platelets) display similar O-glycan structures, comprised mainly of a di-sialylated core 2 hexasaccharide, although the rat GPIbα has a more heterogenous composition of modified sialic acids.
31233555	1	77	theme	tooth	196:200	arg1	surface					202:208	the tooth surface	192:208	the tooth surface	192:208	Streptococcus gordonii and Streptococcus sanguinis are primary colonizers of the tooth surface.
31233555	6	78	dep	type	925:928	arg1	the					921:923	the	921:923	the	921:923	Here we generated isogenic strains of S. gordonii that differ only in the type and range of sialoglycan structures to which they adhere and examined whether this rendered them more or less virulent in a rat model of endocarditis.
31233555	3	79	theme	repeat	372:377	arg1	adhesin					387:393	a serine-rich repeat surface adhesin	358:393	a serine-rich repeat surface adhesin that mediates attachment to sialylated glycans on mucin-like glycoproteins	358:468	Both streptococcal species express a serine-rich repeat surface adhesin that mediates attachment to sialylated glycans on mucin-like glycoproteins, but the specific sialoglycan structures recognized can vary from strain to strain.
31233555	4	80	theme	Previous	554:561	arg1	studies					563:569	Previous studies	554:569	Previous studies	554:569	Previous studies have shown that sialoglycan binding is clearly important for aortic valve infections caused by some S. gordonii, but this process did not contribute to the virulence of a strain of S. sanguinis.
31233555	1	81	theme	primary	170:176	arg1	gordonii					129:136	Streptococcus gordonii	115:136	Streptococcus gordonii	115:136	Streptococcus gordonii and Streptococcus sanguinis are primary colonizers of the tooth surface.
31233555	1	81	theme	primary	170:176	arg1	sanguinis					156:164	Streptococcus sanguinis	142:164	Streptococcus sanguinis	142:164	Streptococcus gordonii and Streptococcus sanguinis are primary colonizers of the tooth surface.
31233555	1	81	theme	primary	170:176	arg1	colonizers					178:187	primary colonizers	170:187	primary colonizers of the tooth surface	170:208	Streptococcus gordonii and Streptococcus sanguinis are primary colonizers of the tooth surface.
31233555	11	82	theme	pathogenic	2168:2177	arg1	effects					2179:2185	pathogenic effects	2168:2185	pathogenic effects	2168:2185	The combined results suggest that streptococcal interaction with a minor O-glycan on GPIbα may be more important than the over-all affinity for GPIbα for pathogenic effects.
31233555	9	83	theme	initial	1468:1474	arg1	proportional					1534:1545	proportional	1534:1545	proportional	1534:1545	A surprising finding was that the initial attachment of streptococci to aortic valves was inversely proportional to the affinity of each strain for platelets, suggesting that binding to platelets circulating in the blood may divert bacteria away from the endocardial surface.
31233555	9	83	theme	initial	1468:1474	arg1	attachment					1476:1485	the initial attachment	1464:1485	the initial attachment of streptococci to aortic valves	1464:1518	A surprising finding was that the initial attachment of streptococci to aortic valves was inversely proportional to the affinity of each strain for platelets, suggesting that binding to platelets circulating in the blood may divert bacteria away from the endocardial surface.
31099733	8	0	theme	polar	1045:1049	arg1	lipids					1051:1056	The polar lipids	1041:1056	The polar lipids	1041:1056	The polar lipids consisted of diphosphatidylglycerol, an unidentified glycolipid and an unidentified lipid.
31099733	4	1	theme	acids	817:821	arg1	acids					817:821	the total fatty acids	801:821	the total fatty acids	801:821	The predominant fatty acids (>10 % of the total fatty acids) were anteiso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0.
31099733	4	1	theme	acids	817:821	arg1	%					796:796	>10 %	792:796	>10 % of the total fatty acids	792:821	The predominant fatty acids (>10 % of the total fatty acids) were anteiso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0.
31099733	7	2	theme	murein	1020:1025	arg1	glycolyl					1031:1038	glycolyl	1031:1038	glycolyl	1031:1038	The N-acyl type of the murein was glycolyl.
31099733	7	2	theme	murein	1020:1025	arg1	type					1008:1011	The N-acyl type	997:1011	The N-acyl type of the murein	997:1025	The N-acyl type of the murein was glycolyl.
31099733	10	3	theme	novel	1320:1324	arg1	genus					1326:1330	a novel genus	1318:1330	a novel genus	1318:1330	Based on its phylogenetic distinctiveness and distinguishing phenotypic characteristics, we conclude that strain 2DFWR-13T represents a novel genus and species of the family Microbacteriaceae, for which the name Protaetiibacter intestinalis gen. nov., sp.
31099733	0	4	theme	kribbensis	208:217	arg1	comb					230:233	comb	230:233	comb	230:233	Protaetiibacter intestinalis gen. nov., of the family Microbacteriaceae, isolated from gut of Protaetia brevitarsis seulensis, reclassification of Lysinimonas kribbensis Jang et al. 2013 as Pseudolysinimonas kribbensis gen. nov., comb.
31099733	0	4	theme	kribbensis	208:217	arg1	nov.					224:227	Pseudolysinimonas kribbensis gen. nov.	190:227	Pseudolysinimonas kribbensis gen. nov.	190:227	Protaetiibacter intestinalis gen. nov., of the family Microbacteriaceae, isolated from gut of Protaetia brevitarsis seulensis, reclassification of Lysinimonas kribbensis Jang et al. 2013 as Pseudolysinimonas kribbensis gen. nov., comb.
31099733	10	5	theme	family	1351:1356	arg1	Microbacteriaceae					1358:1374	the family Microbacteriaceae	1347:1374	the family Microbacteriaceae	1347:1374	Based on its phylogenetic distinctiveness and distinguishing phenotypic characteristics, we conclude that strain 2DFWR-13T represents a novel genus and species of the family Microbacteriaceae, for which the name Protaetiibacter intestinalis gen. nov., sp.
31099733	4	6	theme	total	805:809	arg1	acids					817:821	the total fatty acids	801:821	the total fatty acids	801:821	The predominant fatty acids (>10 % of the total fatty acids) were anteiso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0.
31099733	3	7	theme	ginsengi	730:737	arg1	4.3491T					745:751	Herbiconiux ginsengi CGMCC 4.3491T	718:751	Herbiconiux ginsengi CGMCC 4.3491T (97.1 %)	718:760	Strain 2DFWR-13T showed high sequence similarities to Lysinimonas kribbensis MSL-13T (97.7 %), Homoserinibacter gongjuensis 5GH26-15T (97.2 %), Microbacterium deminutum KV-483T (97.1 %) and Herbiconiux ginsengi CGMCC 4.3491T (97.1 %).
31099733	3	7	theme	ginsengi	730:737	arg1	%					759:759	97.1 %	754:759	97.1 %	754:759	Strain 2DFWR-13T showed high sequence similarities to Lysinimonas kribbensis MSL-13T (97.7 %), Homoserinibacter gongjuensis 5GH26-15T (97.2 %), Microbacterium deminutum KV-483T (97.1 %) and Herbiconiux ginsengi CGMCC 4.3491T (97.1 %).
31099733	13	8	theme	kribbensis	1713:1722	arg1	comb					1735:1738	comb	1735:1738	comb	1735:1738	In addition, an emended description of the genus LysinimonasJang et al. 2013 and the reclassification of Lysinimonas kribbensisJang et al. 2013 as Pseudolysinimonas kribbensis gen. nov., comb.
31099733	13	8	theme	kribbensis	1713:1722	arg1	nov.					1729:1732	Pseudolysinimonas kribbensis gen. nov.	1695:1732	Pseudolysinimonas kribbensis gen. nov.	1695:1732	In addition, an emended description of the genus LysinimonasJang et al. 2013 and the reclassification of Lysinimonas kribbensisJang et al. 2013 as Pseudolysinimonas kribbensis gen. nov., comb.
31099733	3	9	theme	high	552:555	arg1	similarities					566:577	high sequence similarities	552:577	high sequence similarities to Lysinimonas kribbensis MSL-13T (97.7 %), Homoserinibacter gongjuensis 5GH26-15T (97.2 %), Microbacterium deminutum KV-483T (97.1 %) and Herbiconiux ginsengi CGMCC 4.3491T (97.1 %)	552:760	Strain 2DFWR-13T showed high sequence similarities to Lysinimonas kribbensis MSL-13T (97.7 %), Homoserinibacter gongjuensis 5GH26-15T (97.2 %), Microbacterium deminutum KV-483T (97.1 %) and Herbiconiux ginsengi CGMCC 4.3491T (97.1 %).
31099733	9	10	theme	DNA	1153:1155	arg1	G+C content					1157:1167	The DNA G+C content	1149:1167	The DNA G+C content	1149:1167	The DNA G+C content was 71.5 mol%.
31099733	9	10	theme	DNA	1153:1155	arg1	%					1181:1181	71.5 mol%	1173:1181	71.5 mol%	1173:1181	The DNA G+C content was 71.5 mol%.
31099733	13	11	dep	LysinimonasJang	1597:1611	arg1	al.					1616:1618	LysinimonasJang et al.	1597:1618	the genus LysinimonasJang et al. 2013	1587:1623	In addition, an emended description of the genus LysinimonasJang et al. 2013 and the reclassification of Lysinimonas kribbensisJang et al. 2013 as Pseudolysinimonas kribbensis gen. nov., comb.
31099733	3	12	theme	Lysinimonas	582:592	arg1	MSL-13T					605:611	Lysinimonas kribbensis MSL-13T	582:611	Lysinimonas kribbensis MSL-13T (97.7 %)	582:620	Strain 2DFWR-13T showed high sequence similarities to Lysinimonas kribbensis MSL-13T (97.7 %), Homoserinibacter gongjuensis 5GH26-15T (97.2 %), Microbacterium deminutum KV-483T (97.1 %) and Herbiconiux ginsengi CGMCC 4.3491T (97.1 %).
31099733	3	12	theme	Lysinimonas	582:592	arg1	%					619:619	97.7 %	614:619	97.7 %	614:619	Strain 2DFWR-13T showed high sequence similarities to Lysinimonas kribbensis MSL-13T (97.7 %), Homoserinibacter gongjuensis 5GH26-15T (97.2 %), Microbacterium deminutum KV-483T (97.1 %) and Herbiconiux ginsengi CGMCC 4.3491T (97.1 %).
31099733	0	13	dep	Jang	170:173	arg1	al.					178:180	Lysinimonas kribbensis Jang et al.	147:180	Lysinimonas kribbensis Jang et al. 2013	147:185	Protaetiibacter intestinalis gen. nov., of the family Microbacteriaceae, isolated from gut of Protaetia brevitarsis seulensis, reclassification of Lysinimonas kribbensis Jang et al. 2013 as Pseudolysinimonas kribbensis gen. nov., comb.
31099733	12	14	theme	=KACC	1520:1524	arg1	2DFWR-13T					1509:1517	2DFWR-13T	1509:1517	2DFWR-13T (=KACC 19321T=NBRC 113050T)	1509:1545	The type strain of Protaetiibacter intestinalis is 2DFWR-13T (=KACC 19321T=NBRC 113050T).
31099733	12	14	theme	=KACC	1520:1524	arg1	113050T					1538:1544	=KACC 19321T=NBRC 113050T	1520:1544	=KACC 19321T=NBRC 113050T	1520:1544	The type strain of Protaetiibacter intestinalis is 2DFWR-13T (=KACC 19321T=NBRC 113050T).
31099733	0	15	theme	Jang	170:173	arg1	2013					182:185	Lysinimonas kribbensis Jang et al. 2013	147:185	Lysinimonas kribbensis Jang et al. 2013	147:185	Protaetiibacter intestinalis gen. nov., of the family Microbacteriaceae, isolated from gut of Protaetia brevitarsis seulensis, reclassification of Lysinimonas kribbensis Jang et al. 2013 as Pseudolysinimonas kribbensis gen. nov., comb.
31099733	12	16	theme	intestinalis	1493:1504	arg1	2DFWR-13T					1509:1517	2DFWR-13T	1509:1517	2DFWR-13T (=KACC 19321T=NBRC 113050T)	1509:1545	The type strain of Protaetiibacter intestinalis is 2DFWR-13T (=KACC 19321T=NBRC 113050T).
31099733	12	16	theme	intestinalis	1493:1504	arg1	strain					1467:1472	The type strain	1458:1472	The type strain of Protaetiibacter intestinalis	1458:1504	The type strain of Protaetiibacter intestinalis is 2DFWR-13T (=KACC 19321T=NBRC 113050T).
31099733	4	17	dep	anteiso-C15 	829:840	arg1	 0					842:843	 0	842:843	 0	842:843	The predominant fatty acids (>10 % of the total fatty acids) were anteiso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0.
31099733	4	17	dep	anteiso-C15 	829:840	arg1	 0					875:876	 0	875:876	 0	875:876	The predominant fatty acids (>10 % of the total fatty acids) were anteiso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0.
31099733	4	17	dep	anteiso-C15 	829:840	arg1	anteiso-C17 					862:873	anteiso-C17 	862:873	anteiso-C17 	862:873	The predominant fatty acids (>10 % of the total fatty acids) were anteiso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0.
31099733	4	17	dep	anteiso-C15 	829:840	arg1	 0					855:856	 0	855:856	 0	855:856	The predominant fatty acids (>10 % of the total fatty acids) were anteiso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0.
31099733	5	18	theme	major	883:887	arg1	MK-13					907:911	MK-13	907:911	MK-13	907:911	The major menaquinones were MK-13 and MK-12.
31099733	5	18	theme	major	883:887	arg1	menaquinones					889:900	The major menaquinones	879:900	The major menaquinones	879:900	The major menaquinones were MK-13 and MK-12.
31099733	12	19	theme	type	1462:1465	arg1	2DFWR-13T					1509:1517	2DFWR-13T	1509:1517	2DFWR-13T (=KACC 19321T=NBRC 113050T)	1509:1545	The type strain of Protaetiibacter intestinalis is 2DFWR-13T (=KACC 19321T=NBRC 113050T).
31099733	12	19	theme	type	1462:1465	arg1	strain					1467:1472	The type strain	1458:1472	The type strain of Protaetiibacter intestinalis	1458:1504	The type strain of Protaetiibacter intestinalis is 2DFWR-13T (=KACC 19321T=NBRC 113050T).
31099733	13	20	theme	LysinimonasJang	1597:1611	arg1	2013					1620:1623	the genus LysinimonasJang et al. 2013	1587:1623	the genus LysinimonasJang et al. 2013	1587:1623	In addition, an emended description of the genus LysinimonasJang et al. 2013 and the reclassification of Lysinimonas kribbensisJang et al. 2013 as Pseudolysinimonas kribbensis gen. nov., comb.
31099733	0	21	theme	Protaetia	94:102	arg1	gut					87:89	gut	87:89	gut of Protaetia brevitarsis seulensis, reclassification of Lysinimonas kribbensis Jang et al. 2013	87:185	Protaetiibacter intestinalis gen. nov., of the family Microbacteriaceae, isolated from gut of Protaetia brevitarsis seulensis, reclassification of Lysinimonas kribbensis Jang et al. 2013 as Pseudolysinimonas kribbensis gen. nov., comb.
31099733	6	22	theme	acid	985:988	arg1	d-DAB					990:994	the diagnostic amino acid d-DAB	964:994	the diagnostic amino acid d-DAB	964:994	The peptidoglycan type was type B2 with the diagnostic amino acid d-DAB.
31099733	1	23	dep	2013	302:305	arg1	Jang					290:293	Jang	290:293	Jang	290:293	nov. and emended description of the genus Lysinimonas Jang et al. 2013.
31099733	10	24	theme	name	1391:1394	arg1	nov.					1430:1433	the name Protaetiibacter intestinalis gen. nov.	1387:1433	the name Protaetiibacter intestinalis gen. nov.	1387:1433	Based on its phylogenetic distinctiveness and distinguishing phenotypic characteristics, we conclude that strain 2DFWR-13T represents a novel genus and species of the family Microbacteriaceae, for which the name Protaetiibacter intestinalis gen. nov., sp.
31099733	0	25	theme	Protaetiibacter	0:14	arg1	nov.					34:37	Protaetiibacter intestinalis gen. nov.	0:37	Protaetiibacter intestinalis gen. nov.	0:37	Protaetiibacter intestinalis gen. nov., of the family Microbacteriaceae, isolated from gut of Protaetia brevitarsis seulensis, reclassification of Lysinimonas kribbensis Jang et al. 2013 as Pseudolysinimonas kribbensis gen. nov., comb.
31099733	1	26	theme	genus	272:276	arg1	nov.					236:239	nov.	236:239	nov.	236:239	nov. and emended description of the genus Lysinimonas Jang et al. 2013.
31099733	1	26	theme	genus	272:276	arg1	description					253:263	emended description	245:263	emended description of the genus	245:276	nov. and emended description of the genus Lysinimonas Jang et al. 2013.
31099733	7	27	theme	N-acyl	1001:1006	arg1	glycolyl					1031:1038	glycolyl	1031:1038	glycolyl	1031:1038	The N-acyl type of the murein was glycolyl.
31099733	7	27	theme	N-acyl	1001:1006	arg1	type					1008:1011	The N-acyl type	997:1011	The N-acyl type of the murein	997:1025	The N-acyl type of the murein was glycolyl.
31099733	6	28	theme	diagnostic	968:977	arg1	d-DAB					990:994	the diagnostic amino acid d-DAB	964:994	the diagnostic amino acid d-DAB	964:994	The peptidoglycan type was type B2 with the diagnostic amino acid d-DAB.
31099733	10	29	theme	distinguishing	1230:1243	arg1	characteristics					1256:1270	distinguishing phenotypic characteristics	1230:1270	distinguishing phenotypic characteristics	1230:1270	Based on its phylogenetic distinctiveness and distinguishing phenotypic characteristics, we conclude that strain 2DFWR-13T represents a novel genus and species of the family Microbacteriaceae, for which the name Protaetiibacter intestinalis gen. nov., sp.
31099733	0	30	theme	gen.	29:32	arg1	nov.					34:37	Protaetiibacter intestinalis gen. nov.	0:37	Protaetiibacter intestinalis gen. nov.	0:37	Protaetiibacter intestinalis gen. nov., of the family Microbacteriaceae, isolated from gut of Protaetia brevitarsis seulensis, reclassification of Lysinimonas kribbensis Jang et al. 2013 as Pseudolysinimonas kribbensis gen. nov., comb.
31099733	2	31	theme	strain	413:418	arg1	2DFWR-13T					420:428	strain 2DFWR-13T	413:428	strain 2DFWR-13T	413:428	A Gram-stain-positive, strictly aerobic, rod-shaped, non-spore-forming, non-motile bacterium, designated strain 2DFWR-13T, was isolated from gut of the larva of Protaetia brevitarsis seulensis, in the Republic of Korea.
31099733	6	32	theme	type	951:954	arg1	type					942:945	The peptidoglycan type	924:945	The peptidoglycan type	924:945	The peptidoglycan type was type B2 with the diagnostic amino acid d-DAB.
31099733	6	32	theme	type	951:954	arg1	B2					956:957	type B2	951:957	type B2	951:957	The peptidoglycan type was type B2 with the diagnostic amino acid d-DAB.
31099733	3	33	theme	Homoserinibacter	623:638	arg1	5GH26-15T					652:660	Homoserinibacter gongjuensis 5GH26-15T	623:660	Homoserinibacter gongjuensis 5GH26-15T (97.2 %)	623:669	Strain 2DFWR-13T showed high sequence similarities to Lysinimonas kribbensis MSL-13T (97.7 %), Homoserinibacter gongjuensis 5GH26-15T (97.2 %), Microbacterium deminutum KV-483T (97.1 %) and Herbiconiux ginsengi CGMCC 4.3491T (97.1 %).
31099733	3	33	theme	Homoserinibacter	623:638	arg1	%					668:668	97.2 %	663:668	97.2 %	663:668	Strain 2DFWR-13T showed high sequence similarities to Lysinimonas kribbensis MSL-13T (97.7 %), Homoserinibacter gongjuensis 5GH26-15T (97.2 %), Microbacterium deminutum KV-483T (97.1 %) and Herbiconiux ginsengi CGMCC 4.3491T (97.1 %).
31099733	13	34	theme	genus	1591:1595	arg1	2013					1620:1623	the genus LysinimonasJang et al. 2013	1587:1623	the genus LysinimonasJang et al. 2013	1587:1623	In addition, an emended description of the genus LysinimonasJang et al. 2013 and the reclassification of Lysinimonas kribbensisJang et al. 2013 as Pseudolysinimonas kribbensis gen. nov., comb.
31099733	2	35	dep	Protaetia	469:477	arg1	seulensis					491:499	Protaetia brevitarsis seulensis	469:499	Protaetia brevitarsis seulensis	469:499	A Gram-stain-positive, strictly aerobic, rod-shaped, non-spore-forming, non-motile bacterium, designated strain 2DFWR-13T, was isolated from gut of the larva of Protaetia brevitarsis seulensis, in the Republic of Korea.
31099733	6	36	with	B2	956:957	arg1	d-DAB					990:994	the diagnostic amino acid d-DAB	964:994	the diagnostic amino acid d-DAB	964:994	The peptidoglycan type was type B2 with the diagnostic amino acid d-DAB.
31099733	0	37	dep	Protaetia	94:102	arg1	reclassification					127:142	reclassification	127:142	reclassification	127:142	Protaetiibacter intestinalis gen. nov., of the family Microbacteriaceae, isolated from gut of Protaetia brevitarsis seulensis, reclassification of Lysinimonas kribbensis Jang et al. 2013 as Pseudolysinimonas kribbensis gen. nov., comb.
31099733	0	37	dep	Protaetia	94:102	arg1	seulensis					116:124	Protaetia brevitarsis seulensis, reclassification	94:142	seulensis	116:124	Protaetiibacter intestinalis gen. nov., of the family Microbacteriaceae, isolated from gut of Protaetia brevitarsis seulensis, reclassification of Lysinimonas kribbensis Jang et al. 2013 as Pseudolysinimonas kribbensis gen. nov., comb.
31099733	4	38	theme	predominant	767:777	arg1	acids					785:789	The predominant fatty acids	763:789	The predominant fatty acids (>10 % of the total fatty acids)	763:822	The predominant fatty acids (>10 % of the total fatty acids) were anteiso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0.
31099733	4	38	theme	predominant	767:777	arg1	anteiso-C15 					829:840	anteiso-C15 	829:840	anteiso-C15 	829:840	The predominant fatty acids (>10 % of the total fatty acids) were anteiso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0.
31099733	0	39	theme	2013	182:185	arg1	gut					87:89	gut	87:89	gut of Protaetia brevitarsis seulensis, reclassification of Lysinimonas kribbensis Jang et al. 2013	87:185	Protaetiibacter intestinalis gen. nov., of the family Microbacteriaceae, isolated from gut of Protaetia brevitarsis seulensis, reclassification of Lysinimonas kribbensis Jang et al. 2013 as Pseudolysinimonas kribbensis gen. nov., comb.
31099733	1	40	theme	emended	245:251	arg1	description					253:263	emended description	245:263	emended description of the genus	245:276	nov. and emended description of the genus Lysinimonas Jang et al. 2013.
31099733	4	41	theme	fatty	811:815	arg1	acids					817:821	the total fatty acids	801:821	the total fatty acids	801:821	The predominant fatty acids (>10 % of the total fatty acids) were anteiso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0.
31099733	13	42	theme	gen.	1724:1727	arg1	comb					1735:1738	comb	1735:1738	comb	1735:1738	In addition, an emended description of the genus LysinimonasJang et al. 2013 and the reclassification of Lysinimonas kribbensisJang et al. 2013 as Pseudolysinimonas kribbensis gen. nov., comb.
31099733	13	42	theme	gen.	1724:1727	arg1	nov.					1729:1732	Pseudolysinimonas kribbensis gen. nov.	1695:1732	Pseudolysinimonas kribbensis gen. nov.	1695:1732	In addition, an emended description of the genus LysinimonasJang et al. 2013 and the reclassification of Lysinimonas kribbensisJang et al. 2013 as Pseudolysinimonas kribbensis gen. nov., comb.
31099733	3	43	theme	Microbacterium	672:685	arg1	KV-483T					697:703	Microbacterium deminutum KV-483T	672:703	Microbacterium deminutum KV-483T (97.1 %)	672:712	Strain 2DFWR-13T showed high sequence similarities to Lysinimonas kribbensis MSL-13T (97.7 %), Homoserinibacter gongjuensis 5GH26-15T (97.2 %), Microbacterium deminutum KV-483T (97.1 %) and Herbiconiux ginsengi CGMCC 4.3491T (97.1 %).
31099733	3	43	theme	Microbacterium	672:685	arg1	%					711:711	97.1 %	706:711	97.1 %	706:711	Strain 2DFWR-13T showed high sequence similarities to Lysinimonas kribbensis MSL-13T (97.7 %), Homoserinibacter gongjuensis 5GH26-15T (97.2 %), Microbacterium deminutum KV-483T (97.1 %) and Herbiconiux ginsengi CGMCC 4.3491T (97.1 %).
31099733	0	44	theme	Pseudolysinimonas	190:206	arg1	comb					230:233	comb	230:233	comb	230:233	Protaetiibacter intestinalis gen. nov., of the family Microbacteriaceae, isolated from gut of Protaetia brevitarsis seulensis, reclassification of Lysinimonas kribbensis Jang et al. 2013 as Pseudolysinimonas kribbensis gen. nov., comb.
31099733	0	44	theme	Pseudolysinimonas	190:206	arg1	nov.					224:227	Pseudolysinimonas kribbensis gen. nov.	190:227	Pseudolysinimonas kribbensis gen. nov.	190:227	Protaetiibacter intestinalis gen. nov., of the family Microbacteriaceae, isolated from gut of Protaetia brevitarsis seulensis, reclassification of Lysinimonas kribbensis Jang et al. 2013 as Pseudolysinimonas kribbensis gen. nov., comb.
31099733	3	45	theme	Strain	528:533	arg1	2DFWR-13T					535:543	Strain 2DFWR-13T	528:543	Strain 2DFWR-13T	528:543	Strain 2DFWR-13T showed high sequence similarities to Lysinimonas kribbensis MSL-13T (97.7 %), Homoserinibacter gongjuensis 5GH26-15T (97.2 %), Microbacterium deminutum KV-483T (97.1 %) and Herbiconiux ginsengi CGMCC 4.3491T (97.1 %).
31099733	0	46	theme	gen.	219:222	arg1	comb					230:233	comb	230:233	comb	230:233	Protaetiibacter intestinalis gen. nov., of the family Microbacteriaceae, isolated from gut of Protaetia brevitarsis seulensis, reclassification of Lysinimonas kribbensis Jang et al. 2013 as Pseudolysinimonas kribbensis gen. nov., comb.
31099733	0	46	theme	gen.	219:222	arg1	nov.					224:227	Pseudolysinimonas kribbensis gen. nov.	190:227	Pseudolysinimonas kribbensis gen. nov.	190:227	Protaetiibacter intestinalis gen. nov., of the family Microbacteriaceae, isolated from gut of Protaetia brevitarsis seulensis, reclassification of Lysinimonas kribbensis Jang et al. 2013 as Pseudolysinimonas kribbensis gen. nov., comb.
31099733	3	47	theme	sequence	557:564	arg1	similarities					566:577	high sequence similarities	552:577	high sequence similarities to Lysinimonas kribbensis MSL-13T (97.7 %), Homoserinibacter gongjuensis 5GH26-15T (97.2 %), Microbacterium deminutum KV-483T (97.1 %) and Herbiconiux ginsengi CGMCC 4.3491T (97.1 %)	552:760	Strain 2DFWR-13T showed high sequence similarities to Lysinimonas kribbensis MSL-13T (97.7 %), Homoserinibacter gongjuensis 5GH26-15T (97.2 %), Microbacterium deminutum KV-483T (97.1 %) and Herbiconiux ginsengi CGMCC 4.3491T (97.1 %).
31099733	13	48	theme	Pseudolysinimonas	1695:1711	arg1	comb					1735:1738	comb	1735:1738	comb	1735:1738	In addition, an emended description of the genus LysinimonasJang et al. 2013 and the reclassification of Lysinimonas kribbensisJang et al. 2013 as Pseudolysinimonas kribbensis gen. nov., comb.
31099733	13	48	theme	Pseudolysinimonas	1695:1711	arg1	nov.					1729:1732	Pseudolysinimonas kribbensis gen. nov.	1695:1732	Pseudolysinimonas kribbensis gen. nov.	1695:1732	In addition, an emended description of the genus LysinimonasJang et al. 2013 and the reclassification of Lysinimonas kribbensisJang et al. 2013 as Pseudolysinimonas kribbensis gen. nov., comb.
31099733	3	49	theme	CGMCC	739:743	arg1	4.3491T					745:751	Herbiconiux ginsengi CGMCC 4.3491T	718:751	Herbiconiux ginsengi CGMCC 4.3491T (97.1 %)	718:760	Strain 2DFWR-13T showed high sequence similarities to Lysinimonas kribbensis MSL-13T (97.7 %), Homoserinibacter gongjuensis 5GH26-15T (97.2 %), Microbacterium deminutum KV-483T (97.1 %) and Herbiconiux ginsengi CGMCC 4.3491T (97.1 %).
31099733	3	49	theme	CGMCC	739:743	arg1	%					759:759	97.1 %	754:759	97.1 %	754:759	Strain 2DFWR-13T showed high sequence similarities to Lysinimonas kribbensis MSL-13T (97.7 %), Homoserinibacter gongjuensis 5GH26-15T (97.2 %), Microbacterium deminutum KV-483T (97.1 %) and Herbiconiux ginsengi CGMCC 4.3491T (97.1 %).
31099733	8	50	theme	unidentified	1098:1109	arg1	glycolipid					1111:1120	an unidentified glycolipid	1095:1120	an unidentified glycolipid	1095:1120	The polar lipids consisted of diphosphatidylglycerol, an unidentified glycolipid and an unidentified lipid.
31099733	13	51	theme	Lysinimonas	1653:1663	arg1	reclassification					1633:1648	the reclassification	1629:1648	the reclassification of Lysinimonas	1629:1663	In addition, an emended description of the genus LysinimonasJang et al. 2013 and the reclassification of Lysinimonas kribbensisJang et al. 2013 as Pseudolysinimonas kribbensis gen. nov., comb.
31099733	13	51	theme	Lysinimonas	1653:1663	arg1	description					1572:1582	an emended description	1561:1582	an emended description of the genus LysinimonasJang et al. 2013	1561:1623	In addition, an emended description of the genus LysinimonasJang et al. 2013 and the reclassification of Lysinimonas kribbensisJang et al. 2013 as Pseudolysinimonas kribbensis gen. nov., comb.
31099733	3	52	theme	Herbiconiux	718:728	arg1	4.3491T					745:751	Herbiconiux ginsengi CGMCC 4.3491T	718:751	Herbiconiux ginsengi CGMCC 4.3491T (97.1 %)	718:760	Strain 2DFWR-13T showed high sequence similarities to Lysinimonas kribbensis MSL-13T (97.7 %), Homoserinibacter gongjuensis 5GH26-15T (97.2 %), Microbacterium deminutum KV-483T (97.1 %) and Herbiconiux ginsengi CGMCC 4.3491T (97.1 %).
31099733	3	52	theme	Herbiconiux	718:728	arg1	%					759:759	97.1 %	754:759	97.1 %	754:759	Strain 2DFWR-13T showed high sequence similarities to Lysinimonas kribbensis MSL-13T (97.7 %), Homoserinibacter gongjuensis 5GH26-15T (97.2 %), Microbacterium deminutum KV-483T (97.1 %) and Herbiconiux ginsengi CGMCC 4.3491T (97.1 %).
31099733	3	53	theme	kribbensis	594:603	arg1	MSL-13T					605:611	Lysinimonas kribbensis MSL-13T	582:611	Lysinimonas kribbensis MSL-13T (97.7 %)	582:620	Strain 2DFWR-13T showed high sequence similarities to Lysinimonas kribbensis MSL-13T (97.7 %), Homoserinibacter gongjuensis 5GH26-15T (97.2 %), Microbacterium deminutum KV-483T (97.1 %) and Herbiconiux ginsengi CGMCC 4.3491T (97.1 %).
31099733	3	53	theme	kribbensis	594:603	arg1	%					619:619	97.7 %	614:619	97.7 %	614:619	Strain 2DFWR-13T showed high sequence similarities to Lysinimonas kribbensis MSL-13T (97.7 %), Homoserinibacter gongjuensis 5GH26-15T (97.2 %), Microbacterium deminutum KV-483T (97.1 %) and Herbiconiux ginsengi CGMCC 4.3491T (97.1 %).
31099733	4	54	dep	acids	785:789	arg1	acids					817:821	the total fatty acids	801:821	the total fatty acids	801:821	The predominant fatty acids (>10 % of the total fatty acids) were anteiso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0.
31099733	4	54	dep	acids	785:789	arg1	%					796:796	>10 %	792:796	>10 % of the total fatty acids	792:821	The predominant fatty acids (>10 % of the total fatty acids) were anteiso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0.
31099733	2	55	theme	Korea	521:525	arg1	Republic					509:516	the Republic	505:516	the Republic of Korea	505:525	A Gram-stain-positive, strictly aerobic, rod-shaped, non-spore-forming, non-motile bacterium, designated strain 2DFWR-13T, was isolated from gut of the larva of Protaetia brevitarsis seulensis, in the Republic of Korea.
31099733	13	56	theme	et	1680:1681	arg1	2013					1687:1690	et al. 2013	1680:1690	et al. 2013	1680:1690	In addition, an emended description of the genus LysinimonasJang et al. 2013 and the reclassification of Lysinimonas kribbensisJang et al. 2013 as Pseudolysinimonas kribbensis gen. nov., comb.
31099733	8	57	theme	unidentified	1129:1140	arg1	lipid					1142:1146	an unidentified lipid	1126:1146	an unidentified lipid	1126:1146	The polar lipids consisted of diphosphatidylglycerol, an unidentified glycolipid and an unidentified lipid.
31099733	12	58	theme	19321T=NBRC	1526:1536	arg1	2DFWR-13T					1509:1517	2DFWR-13T	1509:1517	2DFWR-13T (=KACC 19321T=NBRC 113050T)	1509:1545	The type strain of Protaetiibacter intestinalis is 2DFWR-13T (=KACC 19321T=NBRC 113050T).
31099733	12	58	theme	19321T=NBRC	1526:1536	arg1	113050T					1538:1544	=KACC 19321T=NBRC 113050T	1520:1544	=KACC 19321T=NBRC 113050T	1520:1544	The type strain of Protaetiibacter intestinalis is 2DFWR-13T (=KACC 19321T=NBRC 113050T).
31099733	9	59	theme	71.5 mol	1173:1180	arg1	G+C content					1157:1167	The DNA G+C content	1149:1167	The DNA G+C content	1149:1167	The DNA G+C content was 71.5 mol%.
31099733	9	59	theme	71.5 mol	1173:1180	arg1	%					1181:1181	71.5 mol%	1173:1181	71.5 mol%	1173:1181	The DNA G+C content was 71.5 mol%.
31099733	12	60	theme	Protaetiibacter	1477:1491	arg1	intestinalis					1493:1504	Protaetiibacter intestinalis	1477:1504	Protaetiibacter intestinalis	1477:1504	The type strain of Protaetiibacter intestinalis is 2DFWR-13T (=KACC 19321T=NBRC 113050T).
31099733	10	61	theme	intestinalis	1412:1423	arg1	nov.					1430:1433	the name Protaetiibacter intestinalis gen. nov.	1387:1433	the name Protaetiibacter intestinalis gen. nov.	1387:1433	Based on its phylogenetic distinctiveness and distinguishing phenotypic characteristics, we conclude that strain 2DFWR-13T represents a novel genus and species of the family Microbacteriaceae, for which the name Protaetiibacter intestinalis gen. nov., sp.
31099733	6	62	theme	peptidoglycan	928:940	arg1	type					942:945	The peptidoglycan type	924:945	The peptidoglycan type	924:945	The peptidoglycan type was type B2 with the diagnostic amino acid d-DAB.
31099733	6	62	theme	peptidoglycan	928:940	arg1	B2					956:957	type B2	951:957	type B2	951:957	The peptidoglycan type was type B2 with the diagnostic amino acid d-DAB.
31099733	1	63	dep	Jang	290:293	arg1	al.					298:300	nov. and emended description of the genus Lysinimonas Jang et al.	236:300	nov. and emended description of the genus Lysinimonas Jang et al.	236:300	nov. and emended description of the genus Lysinimonas Jang et al. 2013.
31099733	2	64	theme	Protaetia	469:477	arg1	larva					460:464	the larva	456:464	the larva of Protaetia brevitarsis seulensis	456:499	A Gram-stain-positive, strictly aerobic, rod-shaped, non-spore-forming, non-motile bacterium, designated strain 2DFWR-13T, was isolated from gut of the larva of Protaetia brevitarsis seulensis, in the Republic of Korea.
31099733	10	65	theme	Microbacteriaceae	1358:1374	arg1	genus					1326:1330	a novel genus	1318:1330	a novel genus	1318:1330	Based on its phylogenetic distinctiveness and distinguishing phenotypic characteristics, we conclude that strain 2DFWR-13T represents a novel genus and species of the family Microbacteriaceae, for which the name Protaetiibacter intestinalis gen. nov., sp.
31099733	10	65	theme	Microbacteriaceae	1358:1374	arg1	species					1336:1342	species	1336:1342	species	1336:1342	Based on its phylogenetic distinctiveness and distinguishing phenotypic characteristics, we conclude that strain 2DFWR-13T represents a novel genus and species of the family Microbacteriaceae, for which the name Protaetiibacter intestinalis gen. nov., sp.
31099733	10	66	theme	phylogenetic	1197:1208	arg1	distinctiveness					1210:1224	its phylogenetic distinctiveness	1193:1224	its phylogenetic distinctiveness	1193:1224	Based on its phylogenetic distinctiveness and distinguishing phenotypic characteristics, we conclude that strain 2DFWR-13T represents a novel genus and species of the family Microbacteriaceae, for which the name Protaetiibacter intestinalis gen. nov., sp.
31099733	10	67	dep	sp	1436:1437	arg1	nov.					1430:1433	the name Protaetiibacter intestinalis gen. nov.	1387:1433	the name Protaetiibacter intestinalis gen. nov.	1387:1433	Based on its phylogenetic distinctiveness and distinguishing phenotypic characteristics, we conclude that strain 2DFWR-13T represents a novel genus and species of the family Microbacteriaceae, for which the name Protaetiibacter intestinalis gen. nov., sp.
31099733	13	68	dep	et	1680:1681	arg1	al.					1683:1685	al.	1683:1685	al.	1683:1685	In addition, an emended description of the genus LysinimonasJang et al. 2013 and the reclassification of Lysinimonas kribbensisJang et al. 2013 as Pseudolysinimonas kribbensis gen. nov., comb.
31099733	10	69	theme	Protaetiibacter	1396:1410	arg1	nov.					1430:1433	the name Protaetiibacter intestinalis gen. nov.	1387:1433	the name Protaetiibacter intestinalis gen. nov.	1387:1433	Based on its phylogenetic distinctiveness and distinguishing phenotypic characteristics, we conclude that strain 2DFWR-13T represents a novel genus and species of the family Microbacteriaceae, for which the name Protaetiibacter intestinalis gen. nov., sp.
31099733	0	70	theme	intestinalis	16:27	arg1	nov.					34:37	Protaetiibacter intestinalis gen. nov.	0:37	Protaetiibacter intestinalis gen. nov.	0:37	Protaetiibacter intestinalis gen. nov., of the family Microbacteriaceae, isolated from gut of Protaetia brevitarsis seulensis, reclassification of Lysinimonas kribbensis Jang et al. 2013 as Pseudolysinimonas kribbensis gen. nov., comb.
31099733	6	71	theme	amino	979:983	arg1	d-DAB					990:994	the diagnostic amino acid d-DAB	964:994	the diagnostic amino acid d-DAB	964:994	The peptidoglycan type was type B2 with the diagnostic amino acid d-DAB.
31099733	13	72	theme	2013	1620:1623	arg1	reclassification					1633:1648	the reclassification	1629:1648	the reclassification of Lysinimonas	1629:1663	In addition, an emended description of the genus LysinimonasJang et al. 2013 and the reclassification of Lysinimonas kribbensisJang et al. 2013 as Pseudolysinimonas kribbensis gen. nov., comb.
31099733	13	72	theme	2013	1620:1623	arg1	description					1572:1582	an emended description	1561:1582	an emended description of the genus LysinimonasJang et al. 2013	1561:1623	In addition, an emended description of the genus LysinimonasJang et al. 2013 and the reclassification of Lysinimonas kribbensisJang et al. 2013 as Pseudolysinimonas kribbensis gen. nov., comb.
31099733	2	73	dep	Gram-stain-positive	310:328	arg1	rod-shaped					349:358	rod-shaped	349:358	rod-shaped	349:358	A Gram-stain-positive, strictly aerobic, rod-shaped, non-spore-forming, non-motile bacterium, designated strain 2DFWR-13T, was isolated from gut of the larva of Protaetia brevitarsis seulensis, in the Republic of Korea.
31099733	2	73	dep	Gram-stain-positive	310:328	arg1	aerobic					340:346	aerobic	340:346	aerobic	340:346	A Gram-stain-positive, strictly aerobic, rod-shaped, non-spore-forming, non-motile bacterium, designated strain 2DFWR-13T, was isolated from gut of the larva of Protaetia brevitarsis seulensis, in the Republic of Korea.
31099733	2	73	dep	Gram-stain-positive	310:328	arg1	non-motile					380:389	non-motile	380:389	non-motile	380:389	A Gram-stain-positive, strictly aerobic, rod-shaped, non-spore-forming, non-motile bacterium, designated strain 2DFWR-13T, was isolated from gut of the larva of Protaetia brevitarsis seulensis, in the Republic of Korea.
31099733	2	73	dep	Gram-stain-positive	310:328	arg1	non-spore-forming					361:377	non-spore-forming	361:377	non-spore-forming	361:377	A Gram-stain-positive, strictly aerobic, rod-shaped, non-spore-forming, non-motile bacterium, designated strain 2DFWR-13T, was isolated from gut of the larva of Protaetia brevitarsis seulensis, in the Republic of Korea.
31099733	10	74	theme	phenotypic	1245:1254	arg1	characteristics					1256:1270	distinguishing phenotypic characteristics	1230:1270	distinguishing phenotypic characteristics	1230:1270	Based on its phylogenetic distinctiveness and distinguishing phenotypic characteristics, we conclude that strain 2DFWR-13T represents a novel genus and species of the family Microbacteriaceae, for which the name Protaetiibacter intestinalis gen. nov., sp.
31099733	3	75	dep	Microbacterium	672:685	arg1	deminutum					687:695	deminutum	687:695	deminutum	687:695	Strain 2DFWR-13T showed high sequence similarities to Lysinimonas kribbensis MSL-13T (97.7 %), Homoserinibacter gongjuensis 5GH26-15T (97.2 %), Microbacterium deminutum KV-483T (97.1 %) and Herbiconiux ginsengi CGMCC 4.3491T (97.1 %).
31099733	2	76	attach	isolated	435:442	arg1	gut					449:451	gut	449:451	gut of the larva of Protaetia brevitarsis seulensis	449:499	A Gram-stain-positive, strictly aerobic, rod-shaped, non-spore-forming, non-motile bacterium, designated strain 2DFWR-13T, was isolated from gut of the larva of Protaetia brevitarsis seulensis, in the Republic of Korea.
31099733	2	76	attach	isolated	435:442	arg2	bacterium					391:399	A Gram-stain-positive, strictly aerobic, rod-shaped, non-spore-forming, non-motile bacterium	308:399	A Gram-stain-positive, strictly aerobic, rod-shaped, non-spore-forming, non-motile bacterium	308:399	A Gram-stain-positive, strictly aerobic, rod-shaped, non-spore-forming, non-motile bacterium, designated strain 2DFWR-13T, was isolated from gut of the larva of Protaetia brevitarsis seulensis, in the Republic of Korea.
31099733	3	77	theme	gongjuensis	640:650	arg1	5GH26-15T					652:660	Homoserinibacter gongjuensis 5GH26-15T	623:660	Homoserinibacter gongjuensis 5GH26-15T (97.2 %)	623:669	Strain 2DFWR-13T showed high sequence similarities to Lysinimonas kribbensis MSL-13T (97.7 %), Homoserinibacter gongjuensis 5GH26-15T (97.2 %), Microbacterium deminutum KV-483T (97.1 %) and Herbiconiux ginsengi CGMCC 4.3491T (97.1 %).
31099733	3	77	theme	gongjuensis	640:650	arg1	%					668:668	97.2 %	663:668	97.2 %	663:668	Strain 2DFWR-13T showed high sequence similarities to Lysinimonas kribbensis MSL-13T (97.7 %), Homoserinibacter gongjuensis 5GH26-15T (97.2 %), Microbacterium deminutum KV-483T (97.1 %) and Herbiconiux ginsengi CGMCC 4.3491T (97.1 %).
31099733	2	78	theme	larva	460:464	arg1	gut					449:451	gut	449:451	gut of the larva of Protaetia brevitarsis seulensis	449:499	A Gram-stain-positive, strictly aerobic, rod-shaped, non-spore-forming, non-motile bacterium, designated strain 2DFWR-13T, was isolated from gut of the larva of Protaetia brevitarsis seulensis, in the Republic of Korea.
31099733	10	79	theme	gen.	1425:1428	arg1	nov.					1430:1433	the name Protaetiibacter intestinalis gen. nov.	1387:1433	the name Protaetiibacter intestinalis gen. nov.	1387:1433	Based on its phylogenetic distinctiveness and distinguishing phenotypic characteristics, we conclude that strain 2DFWR-13T represents a novel genus and species of the family Microbacteriaceae, for which the name Protaetiibacter intestinalis gen. nov., sp.
31099733	4	80	theme	fatty	779:783	arg1	acids					785:789	The predominant fatty acids	763:789	The predominant fatty acids (>10 % of the total fatty acids)	763:822	The predominant fatty acids (>10 % of the total fatty acids) were anteiso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0.
31099733	4	80	theme	fatty	779:783	arg1	anteiso-C15 					829:840	anteiso-C15 	829:840	anteiso-C15 	829:840	The predominant fatty acids (>10 % of the total fatty acids) were anteiso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0.
31099733	0	81	theme	family	47:52	arg1	nov.					34:37	Protaetiibacter intestinalis gen. nov.	0:37	Protaetiibacter intestinalis gen. nov.	0:37	Protaetiibacter intestinalis gen. nov., of the family Microbacteriaceae, isolated from gut of Protaetia brevitarsis seulensis, reclassification of Lysinimonas kribbensis Jang et al. 2013 as Pseudolysinimonas kribbensis gen. nov., comb.
31099733	0	82	dep	nov.	34:37	arg1	Microbacteriaceae					54:70	Microbacteriaceae	54:70	Microbacteriaceae	54:70	Protaetiibacter intestinalis gen. nov., of the family Microbacteriaceae, isolated from gut of Protaetia brevitarsis seulensis, reclassification of Lysinimonas kribbensis Jang et al. 2013 as Pseudolysinimonas kribbensis gen. nov., comb.
31099733	13	83	theme	emended	1564:1570	arg1	description					1572:1582	an emended description	1561:1582	an emended description of the genus LysinimonasJang et al. 2013	1561:1623	In addition, an emended description of the genus LysinimonasJang et al. 2013 and the reclassification of Lysinimonas kribbensisJang et al. 2013 as Pseudolysinimonas kribbensis gen. nov., comb.
31099733	2	84	theme	Gram-stain-positive	310:328	arg1	bacterium					391:399	A Gram-stain-positive, strictly aerobic, rod-shaped, non-spore-forming, non-motile bacterium	308:399	A Gram-stain-positive, strictly aerobic, rod-shaped, non-spore-forming, non-motile bacterium	308:399	A Gram-stain-positive, strictly aerobic, rod-shaped, non-spore-forming, non-motile bacterium, designated strain 2DFWR-13T, was isolated from gut of the larva of Protaetia brevitarsis seulensis, in the Republic of Korea.
31099733	10	85	theme	strain	1290:1295	arg1	2DFWR-13T					1297:1305	strain 2DFWR-13T	1290:1305	strain 2DFWR-13T	1290:1305	Based on its phylogenetic distinctiveness and distinguishing phenotypic characteristics, we conclude that strain 2DFWR-13T represents a novel genus and species of the family Microbacteriaceae, for which the name Protaetiibacter intestinalis gen. nov., sp.
29652435	9	0	from	formation	1281:1289	arg1	structures					1333:1342	their structures	1327:1342	their structures	1327:1342	Furthermore, the scanning electron microscopy analysis showed the growth of fungi over solid wastes, the formation of conidiophores and the changes in their structures.
29652435	5	1	theme	biggest	652:658	arg1	increase					660:667	the biggest increase	648:667	the biggest increase	648:667	Aspergillus ibericus was the fungus with the biggest increase of protein, which ranged from 1.4 times to 6.2 times with respect to unfermented wastes.
29652435	9	2	theme	electron	1202:1209	arg1	microscopy					1211:1220	the scanning electron microscopy	1189:1220	the scanning electron microscopy analysis	1189:1229	Furthermore, the scanning electron microscopy analysis showed the growth of fungi over solid wastes, the formation of conidiophores and the changes in their structures.
29652435	2	3	theme	animal	361:366	arg1	feed					368:371	animal feed	361:371	animal feed	361:371	This generates environmental problems, and the waste has a low nutritional value for use as animal feed.
29652435	7	4	theme	SSF	899:901	arg1	substrates					860:869	substrates composition, fungi used and SSF performance	860:913	substrates composition, fungi used and SSF performance	860:913	The relationships among substrates composition, fungi used and SSF performance were evaluated by principal components analysis.
29652435	7	4	theme	SSF	899:901	arg1	performance					903:913	SSF performance	899:913	SSF performance	899:913	The relationships among substrates composition, fungi used and SSF performance were evaluated by principal components analysis.
29652435	0	5	theme	protein	94:100	arg1	production					102:111	protein production	94:111	protein production	94:111	Mediterranean agro-industrial wastes as valuable substrates for lignocellulolytic enzymes and protein production by solid-state fermentation.
29652435	6	6	theme	xylanase	815:822	arg1	activities					824:833	xylanase activities	815:833	xylanase activities	815:833	Likewise, A. ibericus achieved the maximum cellulase and xylanase activities.
29652435	5	7	theme	protein	672:678	arg1	increase					660:667	the biggest increase	648:667	the biggest increase	648:667	Aspergillus ibericus was the fungus with the biggest increase of protein, which ranged from 1.4 times to 6.2 times with respect to unfermented wastes.
29652435	10	8	theme	same	1501:1504	arg1	process					1506:1512	the same process	1497:1512	the same process	1497:1512	CONCLUSION The nutritional value of Mediterranean wastes was improved and other value-added products such as lignocellulolytic enzymes were produced in the same process, which could facilitate the efficient reuse of these wastes.
29652435	0	9	theme	solid-state	116:126	arg1	fermentation					128:139	solid-state fermentation	116:139	solid-state fermentation	116:139	Mediterranean agro-industrial wastes as valuable substrates for lignocellulolytic enzymes and protein production by solid-state fermentation.
29652435	10	10	theme	other	1419:1423	arg1	products					1437:1444	other value-added products	1419:1444	other value-added products such as lignocellulolytic enzymes	1419:1478	CONCLUSION The nutritional value of Mediterranean wastes was improved and other value-added products such as lignocellulolytic enzymes were produced in the same process, which could facilitate the efficient reuse of these wastes.
29652435	10	10	theme	other	1419:1423	arg1	enzymes					1472:1478	lignocellulolytic enzymes	1454:1478	lignocellulolytic enzymes	1454:1478	CONCLUSION The nutritional value of Mediterranean wastes was improved and other value-added products such as lignocellulolytic enzymes were produced in the same process, which could facilitate the efficient reuse of these wastes.
29652435	8	11	theme	cellulose	984:992	arg1	content					973:979	The high content	964:979	The high content of cellulose and hemicellulose	964:1010	The high content of cellulose and hemicellulose favoured lignocellulolytic enzymes production, and the phenolics content was negatively correlated with enzymes production and with the increase of protein by SSF.
29652435	10	12	theme	wastes	1395:1400	arg1	value					1372:1376	The nutritional value	1356:1376	The nutritional value of Mediterranean wastes	1356:1400	CONCLUSION The nutritional value of Mediterranean wastes was improved and other value-added products such as lignocellulolytic enzymes were produced in the same process, which could facilitate the efficient reuse of these wastes.
29652435	1	13	dep	industries	177:186	arg1	such					189:192	such	189:192	such	189:192	BACKGROUND Mediterranean agro-food industries (such as wineries, breweries and olive mills) dispose of great amounts of waste.
29652435	10	14	theme	value-added	1425:1435	arg1	products					1437:1444	other value-added products	1419:1444	other value-added products such as lignocellulolytic enzymes	1419:1478	CONCLUSION The nutritional value of Mediterranean wastes was improved and other value-added products such as lignocellulolytic enzymes were produced in the same process, which could facilitate the efficient reuse of these wastes.
29652435	10	14	theme	value-added	1425:1435	arg1	enzymes					1472:1478	lignocellulolytic enzymes	1454:1478	lignocellulolytic enzymes	1454:1478	CONCLUSION The nutritional value of Mediterranean wastes was improved and other value-added products such as lignocellulolytic enzymes were produced in the same process, which could facilitate the efficient reuse of these wastes.
29652435	3	15	theme	lignocellulolytic	498:514	arg1	enzymes					516:522	lignocellulolytic enzymes	498:522	lignocellulolytic enzymes	498:522	In this sense, solid-state fermentation (SSF) can increase the nutritional value of these wastes and simultaneously produce lignocellulolytic enzymes.
29652435	5	16	with	fungus	636:641	arg1	increase					660:667	the biggest increase	648:667	the biggest increase	648:667	Aspergillus ibericus was the fungus with the biggest increase of protein, which ranged from 1.4 times to 6.2 times with respect to unfermented wastes.
29652435	6	17	theme	maximum	793:799	arg1	cellulase					801:809	the maximum cellulase	789:809	the maximum cellulase	789:809	Likewise, A. ibericus achieved the maximum cellulase and xylanase activities.
29652435	4	18	dep	RESULTS	525:531	arg1	wastes					547:552	All fermented wastes	533:552	RESULTS All fermented wastes	525:552	RESULTS All fermented wastes were enriched in protein by the three fungi studied.
29652435	8	19	theme	enzymes	1039:1045	arg1	production					1047:1056	lignocellulolytic enzymes production	1021:1056	lignocellulolytic enzymes production	1021:1056	The high content of cellulose and hemicellulose favoured lignocellulolytic enzymes production, and the phenolics content was negatively correlated with enzymes production and with the increase of protein by SSF.
29652435	8	20	theme	phenolics	1067:1075	arg1	content					1077:1083	the phenolics content	1063:1083	the phenolics content	1063:1083	The high content of cellulose and hemicellulose favoured lignocellulolytic enzymes production, and the phenolics content was negatively correlated with enzymes production and with the increase of protein by SSF.
29652435	8	21	theme	lignocellulolytic	1021:1037	arg1	production					1047:1056	lignocellulolytic enzymes production	1021:1056	lignocellulolytic enzymes production	1021:1056	The high content of cellulose and hemicellulose favoured lignocellulolytic enzymes production, and the phenolics content was negatively correlated with enzymes production and with the increase of protein by SSF.
29652435	2	22	theme	low	328:330	arg1	value					344:348	a low nutritional value	326:348	a low nutritional value for use as animal feed	326:371	This generates environmental problems, and the waste has a low nutritional value for use as animal feed.
29652435	0	23	theme	Mediterranean	0:12	arg1	agro-industrial					14:28	Mediterranean agro-industrial	0:28	Mediterranean agro-industrial	0:28	Mediterranean agro-industrial wastes as valuable substrates for lignocellulolytic enzymes and protein production by solid-state fermentation.
29652435	7	24	theme	principal	933:941	arg1	analysis					954:961	principal components analysis	933:961	principal components analysis	933:961	The relationships among substrates composition, fungi used and SSF performance were evaluated by principal components analysis.
29652435	1	25	theme	olive	221:225	arg1	mills					227:231	olive mills	221:231	olive mills	221:231	BACKGROUND Mediterranean agro-food industries (such as wineries, breweries and olive mills) dispose of great amounts of waste.
29652435	9	26	theme	fungi	1252:1256	arg1	growth					1242:1247	the growth	1238:1247	the growth of fungi	1238:1256	Furthermore, the scanning electron microscopy analysis showed the growth of fungi over solid wastes, the formation of conidiophores and the changes in their structures.
29652435	7	27	theme	components	943:952	arg1	analysis					954:961	principal components analysis	933:961	principal components analysis	933:961	The relationships among substrates composition, fungi used and SSF performance were evaluated by principal components analysis.
29652435	9	28	theme	microscopy	1211:1220	arg1	analysis					1222:1229	the scanning electron microscopy analysis	1189:1229	the scanning electron microscopy analysis	1189:1229	Furthermore, the scanning electron microscopy analysis showed the growth of fungi over solid wastes, the formation of conidiophores and the changes in their structures.
29652435	8	29	theme	enzymes	1116:1122	arg1	production					1124:1133	enzymes production	1116:1133	enzymes production	1116:1133	The high content of cellulose and hemicellulose favoured lignocellulolytic enzymes production, and the phenolics content was negatively correlated with enzymes production and with the increase of protein by SSF.
29652435	9	30	theme	solid	1263:1267	arg1	wastes					1269:1274	solid wastes	1263:1274	solid wastes	1263:1274	Furthermore, the scanning electron microscopy analysis showed the growth of fungi over solid wastes, the formation of conidiophores and the changes in their structures.
29652435	3	31	theme	nutritional	437:447	arg1	value					449:453	the nutritional value	433:453	the nutritional value of these wastes	433:469	In this sense, solid-state fermentation (SSF) can increase the nutritional value of these wastes and simultaneously produce lignocellulolytic enzymes.
29652435	0	32	theme	valuable	40:47	arg1	substrates					49:58	valuable substrates	40:58	valuable substrates for lignocellulolytic enzymes and protein production	40:111	Mediterranean agro-industrial wastes as valuable substrates for lignocellulolytic enzymes and protein production by solid-state fermentation.
29652435	4	33	theme	fermented	537:545	arg1	wastes					547:552	All fermented wastes	533:552	RESULTS All fermented wastes	525:552	RESULTS All fermented wastes were enriched in protein by the three fungi studied.
29652435	11	34	theme	Chemical	1593:1600	arg1	Industry					1602:1609	Chemical Industry	1593:1609	Chemical Industry	1593:1609	© 2018 Society of Chemical Industry.
29652435	10	35	theme	Mediterranean	1381:1393	arg1	wastes					1395:1400	Mediterranean wastes	1381:1400	Mediterranean wastes	1381:1400	CONCLUSION The nutritional value of Mediterranean wastes was improved and other value-added products such as lignocellulolytic enzymes were produced in the same process, which could facilitate the efficient reuse of these wastes.
29652435	3	36	theme	solid-state	389:399	arg1	fermentation					401:412	solid-state fermentation	389:412	solid-state fermentation (SSF)	389:418	In this sense, solid-state fermentation (SSF) can increase the nutritional value of these wastes and simultaneously produce lignocellulolytic enzymes.
29652435	3	36	theme	solid-state	389:399	arg1	SSF					415:417	SSF	415:417	SSF	415:417	In this sense, solid-state fermentation (SSF) can increase the nutritional value of these wastes and simultaneously produce lignocellulolytic enzymes.
29652435	2	37	contain	has	322:324	arg2	value					344:348	a low nutritional value	326:348	a low nutritional value for use as animal feed	326:371	This generates environmental problems, and the waste has a low nutritional value for use as animal feed.
29652435	2	37	contain	has	322:324	arg1	waste					316:320	the waste	312:320	the waste	312:320	This generates environmental problems, and the waste has a low nutritional value for use as animal feed.
29652435	7	38	dep	substrates	860:869	arg1	substrates					860:869	substrates composition, fungi used and SSF performance	860:913	substrates composition, fungi used and SSF performance	860:913	The relationships among substrates composition, fungi used and SSF performance were evaluated by principal components analysis.
29652435	7	38	dep	substrates	860:869	arg1	fungi					884:888	fungi	884:888	fungi used	884:893	The relationships among substrates composition, fungi used and SSF performance were evaluated by principal components analysis.
29652435	7	38	dep	substrates	860:869	arg1	composition					871:881	composition	871:881	composition	871:881	The relationships among substrates composition, fungi used and SSF performance were evaluated by principal components analysis.
29652435	7	38	dep	substrates	860:869	arg1	performance					903:913	SSF performance	899:913	SSF performance	899:913	The relationships among substrates composition, fungi used and SSF performance were evaluated by principal components analysis.
29652435	1	39	theme	BACKGROUND	142:151	arg1	industries					177:186	BACKGROUND Mediterranean agro-food industries	142:186	BACKGROUND Mediterranean agro-food industries (such as wineries, breweries and olive mills)	142:232	BACKGROUND Mediterranean agro-food industries (such as wineries, breweries and olive mills) dispose of great amounts of waste.
29652435	9	40	from	changes	1316:1322	arg1	structures					1333:1342	their structures	1327:1342	their structures	1327:1342	Furthermore, the scanning electron microscopy analysis showed the growth of fungi over solid wastes, the formation of conidiophores and the changes in their structures.
29652435	10	41	theme	wastes	1567:1572	arg1	reuse					1552:1556	the efficient reuse	1538:1556	the efficient reuse of these wastes	1538:1572	CONCLUSION The nutritional value of Mediterranean wastes was improved and other value-added products such as lignocellulolytic enzymes were produced in the same process, which could facilitate the efficient reuse of these wastes.
29652435	10	42	theme	lignocellulolytic	1454:1470	arg1	enzymes					1472:1478	lignocellulolytic enzymes	1454:1478	lignocellulolytic enzymes	1454:1478	CONCLUSION The nutritional value of Mediterranean wastes was improved and other value-added products such as lignocellulolytic enzymes were produced in the same process, which could facilitate the efficient reuse of these wastes.
29652435	1	43	theme	Mediterranean	153:165	arg1	industries					177:186	BACKGROUND Mediterranean agro-food industries	142:186	BACKGROUND Mediterranean agro-food industries (such as wineries, breweries and olive mills)	142:232	BACKGROUND Mediterranean agro-food industries (such as wineries, breweries and olive mills) dispose of great amounts of waste.
29652435	8	44	theme	protein	1160:1166	arg1	increase					1148:1155	the increase	1144:1155	the increase of protein by SSF	1144:1173	The high content of cellulose and hemicellulose favoured lignocellulolytic enzymes production, and the phenolics content was negatively correlated with enzymes production and with the increase of protein by SSF.
29652435	2	45	theme	environmental	284:296	arg1	problems					298:305	environmental problems	284:305	environmental problems	284:305	This generates environmental problems, and the waste has a low nutritional value for use as animal feed.
29652435	10	46	theme	nutritional	1360:1370	arg1	value					1372:1376	The nutritional value	1356:1376	The nutritional value of Mediterranean wastes	1356:1400	CONCLUSION The nutritional value of Mediterranean wastes was improved and other value-added products such as lignocellulolytic enzymes were produced in the same process, which could facilitate the efficient reuse of these wastes.
29652435	1	47	theme	agro-food	167:175	arg1	industries					177:186	BACKGROUND Mediterranean agro-food industries	142:186	BACKGROUND Mediterranean agro-food industries (such as wineries, breweries and olive mills)	142:232	BACKGROUND Mediterranean agro-food industries (such as wineries, breweries and olive mills) dispose of great amounts of waste.
29652435	1	48	theme	great	245:249	arg1	waste					262:266	waste	262:266	waste	262:266	BACKGROUND Mediterranean agro-food industries (such as wineries, breweries and olive mills) dispose of great amounts of waste.
29652435	1	48	theme	great	245:249	arg1	amounts					251:257	great amounts	245:257	great amounts of waste	245:266	BACKGROUND Mediterranean agro-food industries (such as wineries, breweries and olive mills) dispose of great amounts of waste.
29652435	10	49	dep	CONCLUSION	1345:1354	arg1	produced					1485:1492	produced	1485:1492	were produced in the same process, which could facilitate the efficient reuse of these wastes	1480:1572	CONCLUSION The nutritional value of Mediterranean wastes was improved and other value-added products such as lignocellulolytic enzymes were produced in the same process, which could facilitate the efficient reuse of these wastes.
29652435	10	49	dep	CONCLUSION	1345:1354	arg1	improved					1406:1413	improved	1406:1413	was improved	1402:1413	CONCLUSION The nutritional value of Mediterranean wastes was improved and other value-added products such as lignocellulolytic enzymes were produced in the same process, which could facilitate the efficient reuse of these wastes.
29652435	9	50	theme	conidiophores	1294:1306	arg1	changes					1316:1322	the changes	1312:1322	the changes in their structures	1312:1342	Furthermore, the scanning electron microscopy analysis showed the growth of fungi over solid wastes, the formation of conidiophores and the changes in their structures.
29652435	9	50	theme	conidiophores	1294:1306	arg1	formation					1281:1289	the formation	1277:1289	the formation of conidiophores	1277:1306	Furthermore, the scanning electron microscopy analysis showed the growth of fungi over solid wastes, the formation of conidiophores and the changes in their structures.
29652435	8	51	theme	high	968:971	arg1	content					973:979	The high content	964:979	The high content of cellulose and hemicellulose	964:1010	The high content of cellulose and hemicellulose favoured lignocellulolytic enzymes production, and the phenolics content was negatively correlated with enzymes production and with the increase of protein by SSF.
29652435	2	52	theme	nutritional	332:342	arg1	value					344:348	a low nutritional value	326:348	a low nutritional value for use as animal feed	326:371	This generates environmental problems, and the waste has a low nutritional value for use as animal feed.
29652435	3	53	theme	wastes	464:469	arg1	value					449:453	the nutritional value	433:453	the nutritional value of these wastes	433:469	In this sense, solid-state fermentation (SSF) can increase the nutritional value of these wastes and simultaneously produce lignocellulolytic enzymes.
29652435	5	54	theme	unfermented	738:748	arg1	wastes					750:755	unfermented wastes	738:755	unfermented wastes	738:755	Aspergillus ibericus was the fungus with the biggest increase of protein, which ranged from 1.4 times to 6.2 times with respect to unfermented wastes.
29652435	8	55	theme	hemicellulose	998:1010	arg1	content					973:979	The high content	964:979	The high content of cellulose and hemicellulose	964:1010	The high content of cellulose and hemicellulose favoured lignocellulolytic enzymes production, and the phenolics content was negatively correlated with enzymes production and with the increase of protein by SSF.
29652435	10	56	theme	efficient	1542:1550	arg1	reuse					1552:1556	the efficient reuse	1538:1556	the efficient reuse of these wastes	1538:1572	CONCLUSION The nutritional value of Mediterranean wastes was improved and other value-added products such as lignocellulolytic enzymes were produced in the same process, which could facilitate the efficient reuse of these wastes.
29652435	0	57	theme	lignocellulolytic	64:80	arg1	enzymes					82:88	lignocellulolytic enzymes	64:88	lignocellulolytic enzymes	64:88	Mediterranean agro-industrial wastes as valuable substrates for lignocellulolytic enzymes and protein production by solid-state fermentation.
29652435	9	58	theme	scanning	1193:1200	arg1	microscopy					1211:1220	the scanning electron microscopy	1189:1220	the scanning electron microscopy analysis	1189:1229	Furthermore, the scanning electron microscopy analysis showed the growth of fungi over solid wastes, the formation of conidiophores and the changes in their structures.
29652435	1	59	theme	waste	262:266	arg1	waste					262:266	waste	262:266	waste	262:266	BACKGROUND Mediterranean agro-food industries (such as wineries, breweries and olive mills) dispose of great amounts of waste.
29652435	1	59	theme	waste	262:266	arg1	amounts					251:257	great amounts	245:257	great amounts of waste	245:266	BACKGROUND Mediterranean agro-food industries (such as wineries, breweries and olive mills) dispose of great amounts of waste.
30146298	11	0	theme	casein	1851:1856	arg1	number					1858:1863	casein number	1851:1863	casein number	1851:1863	Consequently, given their strong association, we also tested the effect of casein-to-protein ratio (i.e., casein number).
30146298	10	1	theme	computing	1656:1664	arg1	problems					1666:1673	computing problems	1656:1673	computing problems	1656:1673	The contemporary inclusion of protein and casein in the statistical model did not generate computing problems and allowed us to better characterize the role of protein and casein.
30146298	6	2	theme	milk	1067:1070	arg1	ability					1051:1057	syneresis ability	1041:1057	syneresis ability	1041:1057	The results allowed us to clarify the effect of the major milk nutrients on coagulation, curd firming, and syneresis ability of goat milk.
30146298	6	2	theme	milk	1067:1070	arg1	coagulation					1010:1020	coagulation	1010:1020	coagulation	1010:1020	The results allowed us to clarify the effect of the major milk nutrients on coagulation, curd firming, and syneresis ability of goat milk.
30146298	6	2	theme	milk	1067:1070	arg1	firming					1028:1034	curd firming	1023:1034	curd firming	1023:1034	The results allowed us to clarify the effect of the major milk nutrients on coagulation, curd firming, and syneresis ability of goat milk.
30146298	1	3	theme	coagulation	194:204	arg1	properties					206:215	(1) traditional milk coagulation properties	173:215	(1) traditional milk coagulation properties	173:215	The present study investigated the effect of different levels of fat, protein, and casein on (1) traditional milk coagulation properties, and (2) curd firming over time parameters of 1,272 goat milk samples.
30146298	7	4	theme	delayed	1205:1211	arg1	coagulation					1213:1223	delayed coagulation	1205:1223	delayed coagulation	1205:1223	In particular, milk rich in fat was associated with better coagulation properties, whereas milk rich in protein was associated with delayed coagulation.
30146298	12	5	theme	making	2086:2091	arg1	cheese					2079:2084	cheese making	2079:2091	cheese making	2079:2091	Higher values of casein number led to a general improvement in the coagulation ability of milk, suggesting that casein-to-protein ratio, not just protein or casein, should be considered when milk is destined for cheese making.
30146298	6	6	theme	syneresis	1041:1049	arg1	ability					1051:1057	syneresis ability	1041:1057	syneresis ability	1041:1057	The results allowed us to clarify the effect of the major milk nutrients on coagulation, curd firming, and syneresis ability of goat milk.
30146298	1	7	theme	fat	145:147	arg1	levels					135:140	different levels	125:140	different levels of fat, protein, and casein	125:168	The present study investigated the effect of different levels of fat, protein, and casein on (1) traditional milk coagulation properties, and (2) curd firming over time parameters of 1,272 goat milk samples.
30146298	1	8	dep	properties	206:215	arg1	1					174:174	1	174:174	1	174:174	The present study investigated the effect of different levels of fat, protein, and casein on (1) traditional milk coagulation properties, and (2) curd firming over time parameters of 1,272 goat milk samples.
30146298	1	9	theme	protein	150:156	arg1	levels					135:140	different levels	125:140	different levels of fat, protein, and casein	125:168	The present study investigated the effect of different levels of fat, protein, and casein on (1) traditional milk coagulation properties, and (2) curd firming over time parameters of 1,272 goat milk samples.
30146298	7	10	theme	rich	1169:1172	arg1	milk					1164:1167	milk	1164:1167	milk rich in protein	1164:1183	In particular, milk rich in fat was associated with better coagulation properties, whereas milk rich in protein was associated with delayed coagulation.
30146298	11	11	dep	number	1858:1863	arg1	i.e.					1845:1848	i.e.	1845:1848	i.e.	1845:1848	Consequently, given their strong association, we also tested the effect of casein-to-protein ratio (i.e., casein number).
30146298	9	12	theme	statistical	1460:1470	arg1	model					1472:1476	the statistical model	1456:1476	the statistical model	1456:1476	When only protein or only casein was included in the statistical model, the pattern of coagulation, curd firming, and syneresis was almost indistinguishable.
30146298	3	13	from	effect	588:593	arg1	milk					606:609	milk	606:609	milk	606:609	Traditional milk coagulation properties and modeled curd-firming parameters were analyzed using a mixed model that considered the effect of days in milk, parity, farm, breed, the pendulum of the instrument, and different levels of fat, protein, and casein.
30146298	3	13	from	effect	588:593	arg1	protein					694:700	protein	694:700	protein	694:700	Traditional milk coagulation properties and modeled curd-firming parameters were analyzed using a mixed model that considered the effect of days in milk, parity, farm, breed, the pendulum of the instrument, and different levels of fat, protein, and casein.
30146298	3	13	from	effect	588:593	arg1	parity					612:617	parity	612:617	parity	612:617	Traditional milk coagulation properties and modeled curd-firming parameters were analyzed using a mixed model that considered the effect of days in milk, parity, farm, breed, the pendulum of the instrument, and different levels of fat, protein, and casein.
30146298	3	13	from	effect	588:593	arg1	pendulum					637:644	the pendulum	633:644	the pendulum of the instrument	633:662	Traditional milk coagulation properties and modeled curd-firming parameters were analyzed using a mixed model that considered the effect of days in milk, parity, farm, breed, the pendulum of the instrument, and different levels of fat, protein, and casein.
30146298	3	13	from	effect	588:593	arg1	levels					679:684	different levels	669:684	different levels of fat, protein, and casein	669:712	Traditional milk coagulation properties and modeled curd-firming parameters were analyzed using a mixed model that considered the effect of days in milk, parity, farm, breed, the pendulum of the instrument, and different levels of fat, protein, and casein.
30146298	3	13	from	effect	588:593	arg1	farm					620:623	farm	620:623	farm	620:623	Traditional milk coagulation properties and modeled curd-firming parameters were analyzed using a mixed model that considered the effect of days in milk, parity, farm, breed, the pendulum of the instrument, and different levels of fat, protein, and casein.
30146298	3	13	from	effect	588:593	arg1	casein					707:712	casein	707:712	casein	707:712	Traditional milk coagulation properties and modeled curd-firming parameters were analyzed using a mixed model that considered the effect of days in milk, parity, farm, breed, the pendulum of the instrument, and different levels of fat, protein, and casein.
30146298	3	13	from	effect	588:593	arg1	fat					689:691	fat	689:691	fat	689:691	Traditional milk coagulation properties and modeled curd-firming parameters were analyzed using a mixed model that considered the effect of days in milk, parity, farm, breed, the pendulum of the instrument, and different levels of fat, protein, and casein.
30146298	3	13	from	effect	588:593	arg1	breed					626:630	breed	626:630	breed	626:630	Traditional milk coagulation properties and modeled curd-firming parameters were analyzed using a mixed model that considered the effect of days in milk, parity, farm, breed, the pendulum of the instrument, and different levels of fat, protein, and casein.
30146298	3	13	from	effect	588:593	arg1	instrument					653:662	the instrument	649:662	the instrument	649:662	Traditional milk coagulation properties and modeled curd-firming parameters were analyzed using a mixed model that considered the effect of days in milk, parity, farm, breed, the pendulum of the instrument, and different levels of fat, protein, and casein.
30146298	11	14	theme	ratio	1838:1842	arg1	effect					1810:1815	the effect	1806:1815	the effect of casein-to-protein ratio (i.e., casein number)	1806:1864	Consequently, given their strong association, we also tested the effect of casein-to-protein ratio (i.e., casein number).
30146298	8	15	theme	high	1230:1233	arg1	correlation					1235:1245	The high correlation	1226:1245	The high correlation of fat with protein and casein contents	1226:1285	The high correlation of fat with protein and casein contents suggests that the effect of fat on the cheese-making process is also attributable to the effects of protein and casein.
30146298	3	16	theme	milk	470:473	arg1	properties					487:496	Traditional milk coagulation properties	458:496	Traditional milk coagulation properties	458:496	Traditional milk coagulation properties and modeled curd-firming parameters were analyzed using a mixed model that considered the effect of days in milk, parity, farm, breed, the pendulum of the instrument, and different levels of fat, protein, and casein.
30146298	7	17	from	rich	1169:1172	arg1	protein					1177:1183	protein	1177:1183	protein	1177:1183	In particular, milk rich in fat was associated with better coagulation properties, whereas milk rich in protein was associated with delayed coagulation.
30146298	3	18	theme	curd-firming	510:521	arg1	parameters					523:532	modeled curd-firming parameters	502:532	modeled curd-firming parameters	502:532	Traditional milk coagulation properties and modeled curd-firming parameters were analyzed using a mixed model that considered the effect of days in milk, parity, farm, breed, the pendulum of the instrument, and different levels of fat, protein, and casein.
30146298	7	19	theme	better	1125:1130	arg1	properties					1144:1153	better coagulation properties	1125:1153	better coagulation properties	1125:1153	In particular, milk rich in fat was associated with better coagulation properties, whereas milk rich in protein was associated with delayed coagulation.
30146298	7	20	from	protein	1177:1183	arg1	rich					1169:1172	rich	1169:1172	rich	1169:1172	In particular, milk rich in fat was associated with better coagulation properties, whereas milk rich in protein was associated with delayed coagulation.
30146298	9	21	theme	coagulation	1494:1504	arg1	pattern					1483:1489	the pattern	1479:1489	the pattern of coagulation, curd firming, and syneresis	1479:1533	When only protein or only casein was included in the statistical model, the pattern of coagulation, curd firming, and syneresis was almost indistinguishable.
30146298	9	21	theme	coagulation	1494:1504	arg1	indistinguishable					1546:1562	indistinguishable	1546:1562	indistinguishable	1546:1562	When only protein or only casein was included in the statistical model, the pattern of coagulation, curd firming, and syneresis was almost indistinguishable.
30146298	6	22	theme	milk	992:995	arg1	nutrients					997:1005	the major milk nutrients	982:1005	the major milk nutrients	982:1005	The results allowed us to clarify the effect of the major milk nutrients on coagulation, curd firming, and syneresis ability of goat milk.
30146298	3	23	theme	modeled	502:508	arg1	parameters					523:532	modeled curd-firming parameters	502:532	modeled curd-firming parameters	502:532	Traditional milk coagulation properties and modeled curd-firming parameters were analyzed using a mixed model that considered the effect of days in milk, parity, farm, breed, the pendulum of the instrument, and different levels of fat, protein, and casein.
30146298	9	24	theme	curd	1507:1510	arg1	firming					1512:1518	curd firming	1507:1518	curd firming	1507:1518	When only protein or only casein was included in the statistical model, the pattern of coagulation, curd firming, and syneresis was almost indistinguishable.
30146298	1	25	theme	1,272	263:267	arg1	samples					279:285	1,272 goat milk samples	263:285	1,272 goat milk samples	263:285	The present study investigated the effect of different levels of fat, protein, and casein on (1) traditional milk coagulation properties, and (2) curd firming over time parameters of 1,272 goat milk samples.
30146298	1	26	theme	levels	135:140	arg1	effect					115:120	the effect	111:120	the effect of different levels of fat, protein, and casein on (1) traditional milk coagulation properties, and (2) curd firming	111:237	The present study investigated the effect of different levels of fat, protein, and casein on (1) traditional milk coagulation properties, and (2) curd firming over time parameters of 1,272 goat milk samples.
30146298	3	27	theme	casein	707:712	arg1	milk					606:609	milk	606:609	milk	606:609	Traditional milk coagulation properties and modeled curd-firming parameters were analyzed using a mixed model that considered the effect of days in milk, parity, farm, breed, the pendulum of the instrument, and different levels of fat, protein, and casein.
30146298	3	27	theme	casein	707:712	arg1	protein					694:700	protein	694:700	protein	694:700	Traditional milk coagulation properties and modeled curd-firming parameters were analyzed using a mixed model that considered the effect of days in milk, parity, farm, breed, the pendulum of the instrument, and different levels of fat, protein, and casein.
30146298	3	27	theme	casein	707:712	arg1	parity					612:617	parity	612:617	parity	612:617	Traditional milk coagulation properties and modeled curd-firming parameters were analyzed using a mixed model that considered the effect of days in milk, parity, farm, breed, the pendulum of the instrument, and different levels of fat, protein, and casein.
30146298	3	27	theme	casein	707:712	arg1	pendulum					637:644	the pendulum	633:644	the pendulum of the instrument	633:662	Traditional milk coagulation properties and modeled curd-firming parameters were analyzed using a mixed model that considered the effect of days in milk, parity, farm, breed, the pendulum of the instrument, and different levels of fat, protein, and casein.
30146298	3	27	theme	casein	707:712	arg1	levels					679:684	different levels	669:684	different levels of fat, protein, and casein	669:712	Traditional milk coagulation properties and modeled curd-firming parameters were analyzed using a mixed model that considered the effect of days in milk, parity, farm, breed, the pendulum of the instrument, and different levels of fat, protein, and casein.
30146298	3	27	theme	casein	707:712	arg1	farm					620:623	farm	620:623	farm	620:623	Traditional milk coagulation properties and modeled curd-firming parameters were analyzed using a mixed model that considered the effect of days in milk, parity, farm, breed, the pendulum of the instrument, and different levels of fat, protein, and casein.
30146298	3	27	theme	casein	707:712	arg1	casein					707:712	casein	707:712	casein	707:712	Traditional milk coagulation properties and modeled curd-firming parameters were analyzed using a mixed model that considered the effect of days in milk, parity, farm, breed, the pendulum of the instrument, and different levels of fat, protein, and casein.
30146298	3	27	theme	casein	707:712	arg1	fat					689:691	fat	689:691	fat	689:691	Traditional milk coagulation properties and modeled curd-firming parameters were analyzed using a mixed model that considered the effect of days in milk, parity, farm, breed, the pendulum of the instrument, and different levels of fat, protein, and casein.
30146298	3	27	theme	casein	707:712	arg1	breed					626:630	breed	626:630	breed	626:630	Traditional milk coagulation properties and modeled curd-firming parameters were analyzed using a mixed model that considered the effect of days in milk, parity, farm, breed, the pendulum of the instrument, and different levels of fat, protein, and casein.
30146298	3	27	theme	casein	707:712	arg1	instrument					653:662	the instrument	649:662	the instrument	649:662	Traditional milk coagulation properties and modeled curd-firming parameters were analyzed using a mixed model that considered the effect of days in milk, parity, farm, breed, the pendulum of the instrument, and different levels of fat, protein, and casein.
30146298	4	28	theme	same	766:769	arg1	model					771:775	the same model	762:775	the same model	762:775	Fat, protein, and casein were also tested with the same model but one at a time.
30146298	0	29	from	Effect	0:5	arg1	syneresis					56:64	syneresis	56:64	syneresis of goat milk	56:77	Effect of composition on coagulation, curd firming, and syneresis of goat milk.
30146298	0	29	from	Effect	0:5	arg1	coagulation					25:35	coagulation	25:35	coagulation	25:35	Effect of composition on coagulation, curd firming, and syneresis of goat milk.
30146298	0	29	from	Effect	0:5	arg1	firming					43:49	curd firming	38:49	curd firming	38:49	Effect of composition on coagulation, curd firming, and syneresis of goat milk.
30146298	12	30	theme	milk	1957:1960	arg1	ability					1946:1952	the coagulation ability	1930:1952	the coagulation ability of milk	1930:1960	Higher values of casein number led to a general improvement in the coagulation ability of milk, suggesting that casein-to-protein ratio, not just protein or casein, should be considered when milk is destined for cheese making.
30146298	3	31	theme	different	669:677	arg1	levels					679:684	different levels	669:684	different levels of fat, protein, and casein	669:712	Traditional milk coagulation properties and modeled curd-firming parameters were analyzed using a mixed model that considered the effect of days in milk, parity, farm, breed, the pendulum of the instrument, and different levels of fat, protein, and casein.
30146298	0	32	theme	goat	69:72	arg1	milk					74:77	goat milk	69:77	goat milk	69:77	Effect of composition on coagulation, curd firming, and syneresis of goat milk.
30146298	1	33	theme	milk	274:277	arg1	samples					279:285	1,272 goat milk samples	263:285	1,272 goat milk samples	263:285	The present study investigated the effect of different levels of fat, protein, and casein on (1) traditional milk coagulation properties, and (2) curd firming over time parameters of 1,272 goat milk samples.
30146298	6	34	theme	nutrients	997:1005	arg1	effect					972:977	the effect	968:977	the effect of the major milk nutrients on coagulation, curd firming, and syneresis ability of goat milk	968:1070	The results allowed us to clarify the effect of the major milk nutrients on coagulation, curd firming, and syneresis ability of goat milk.
30146298	1	35	from	effect	115:120	arg1	firming					231:237	(2) curd firming	222:237	(2) curd firming	222:237	The present study investigated the effect of different levels of fat, protein, and casein on (1) traditional milk coagulation properties, and (2) curd firming over time parameters of 1,272 goat milk samples.
30146298	1	35	from	effect	115:120	arg1	properties					206:215	(1) traditional milk coagulation properties	173:215	(1) traditional milk coagulation properties	173:215	The present study investigated the effect of different levels of fat, protein, and casein on (1) traditional milk coagulation properties, and (2) curd firming over time parameters of 1,272 goat milk samples.
30146298	9	36	theme	syneresis	1525:1533	arg1	pattern					1483:1489	the pattern	1479:1489	the pattern of coagulation, curd firming, and syneresis	1479:1533	When only protein or only casein was included in the statistical model, the pattern of coagulation, curd firming, and syneresis was almost indistinguishable.
30146298	9	36	theme	syneresis	1525:1533	arg1	indistinguishable					1546:1562	indistinguishable	1546:1562	indistinguishable	1546:1562	When only protein or only casein was included in the statistical model, the pattern of coagulation, curd firming, and syneresis was almost indistinguishable.
30146298	2	37	theme	udder	358:362	arg1	status					371:376	udder health status	358:376	udder health status (lactose, pH, somatic cells, bacterial count, and NaCl)	358:432	Relationships between fat, protein, and casein and some indicators of udder health status (lactose, pH, somatic cells, bacterial count, and NaCl) were also investigated.
30146298	1	38	dep	firming	231:237	arg1	2					223:223	2	223:223	2	223:223	The present study investigated the effect of different levels of fat, protein, and casein on (1) traditional milk coagulation properties, and (2) curd firming over time parameters of 1,272 goat milk samples.
30146298	2	39	theme	somatic	392:398	arg1	cells					400:404	somatic cells	392:404	somatic cells	392:404	Relationships between fat, protein, and casein and some indicators of udder health status (lactose, pH, somatic cells, bacterial count, and NaCl) were also investigated.
30146298	10	40	from	inclusion	1582:1590	arg1	model					1633:1637	the statistical model	1617:1637	the statistical model	1617:1637	The contemporary inclusion of protein and casein in the statistical model did not generate computing problems and allowed us to better characterize the role of protein and casein.
30146298	8	41	theme	fat	1315:1317	arg1	effect					1305:1310	the effect	1301:1310	the effect of fat on the cheese-making process	1301:1346	The high correlation of fat with protein and casein contents suggests that the effect of fat on the cheese-making process is also attributable to the effects of protein and casein.
30146298	8	41	theme	fat	1315:1317	arg1	attributable					1356:1367	attributable	1356:1367	attributable	1356:1367	The high correlation of fat with protein and casein contents suggests that the effect of fat on the cheese-making process is also attributable to the effects of protein and casein.
30146298	0	42	theme	composition	10:20	arg1	Effect					0:5	Effect	0:5	Effect of composition on coagulation, curd firming, and syneresis of goat milk.	0:78	Effect of composition on coagulation, curd firming, and syneresis of goat milk.
30146298	13	43	theme	dairy	2134:2138	arg1	farmers					2140:2146	dairy farmers	2134:2146	dairy farmers who want to increase their profits by improving the technological quality of the milk produced	2134:2241	These results are especially useful for dairy farmers who want to increase their profits by improving the technological quality of the milk produced.
30146298	8	44	from	effect	1305:1310	arg1	process					1340:1346	the cheese-making process	1322:1346	the cheese-making process	1322:1346	The high correlation of fat with protein and casein contents suggests that the effect of fat on the cheese-making process is also attributable to the effects of protein and casein.
30146298	1	45	theme	curd	226:229	arg1	firming					231:237	(2) curd firming	222:237	(2) curd firming	222:237	The present study investigated the effect of different levels of fat, protein, and casein on (1) traditional milk coagulation properties, and (2) curd firming over time parameters of 1,272 goat milk samples.
30146298	2	46	theme	status	371:376	arg1	indicators					344:353	some indicators	339:353	some indicators of udder health status (lactose, pH, somatic cells, bacterial count, and NaCl)	339:432	Relationships between fat, protein, and casein and some indicators of udder health status (lactose, pH, somatic cells, bacterial count, and NaCl) were also investigated.
30146298	2	46	theme	status	371:376	arg1	Relationships					288:300	Relationships	288:300	Relationships between fat, protein, and casein	288:333	Relationships between fat, protein, and casein and some indicators of udder health status (lactose, pH, somatic cells, bacterial count, and NaCl) were also investigated.
30146298	8	47	with	correlation	1235:1245	arg1	contents					1278:1285	protein and casein contents	1259:1285	protein and casein contents	1259:1285	The high correlation of fat with protein and casein contents suggests that the effect of fat on the cheese-making process is also attributable to the effects of protein and casein.
30146298	0	48	theme	curd	38:41	arg1	firming					43:49	curd firming	38:49	curd firming	38:49	Effect of composition on coagulation, curd firming, and syneresis of goat milk.
30146298	10	49	theme	contemporary	1569:1580	arg1	inclusion					1582:1590	The contemporary inclusion	1565:1590	The contemporary inclusion of protein and casein in the statistical model	1565:1637	The contemporary inclusion of protein and casein in the statistical model did not generate computing problems and allowed us to better characterize the role of protein and casein.
30146298	12	50	theme	casein	1884:1889	arg1	number					1891:1896	casein number	1884:1896	casein number	1884:1896	Higher values of casein number led to a general improvement in the coagulation ability of milk, suggesting that casein-to-protein ratio, not just protein or casein, should be considered when milk is destined for cheese making.
30146298	4	51	from	time	790:793	arg1	one					781:783	one	781:783	one	781:783	Fat, protein, and casein were also tested with the same model but one at a time.
30146298	7	52	from	rich	1093:1096	arg1	fat					1101:1103	fat	1101:1103	fat	1101:1103	In particular, milk rich in fat was associated with better coagulation properties, whereas milk rich in protein was associated with delayed coagulation.
30146298	10	53	theme	statistical	1621:1631	arg1	model					1633:1637	the statistical model	1617:1637	the statistical model	1617:1637	The contemporary inclusion of protein and casein in the statistical model did not generate computing problems and allowed us to better characterize the role of protein and casein.
30146298	7	54	from	fat	1101:1103	arg1	rich					1093:1096	rich	1093:1096	rich	1093:1096	In particular, milk rich in fat was associated with better coagulation properties, whereas milk rich in protein was associated with delayed coagulation.
30146298	3	55	theme	days	598:601	arg1	effect					588:593	the effect	584:593	the effect of days in milk, parity, farm, breed, the pendulum of the instrument, and different levels of fat, protein, and casein	584:712	Traditional milk coagulation properties and modeled curd-firming parameters were analyzed using a mixed model that considered the effect of days in milk, parity, farm, breed, the pendulum of the instrument, and different levels of fat, protein, and casein.
30146298	1	56	theme	milk	189:192	arg1	properties					206:215	(1) traditional milk coagulation properties	173:215	(1) traditional milk coagulation properties	173:215	The present study investigated the effect of different levels of fat, protein, and casein on (1) traditional milk coagulation properties, and (2) curd firming over time parameters of 1,272 goat milk samples.
30146298	8	57	theme	protein	1387:1393	arg1	effects					1376:1382	the effects	1372:1382	the effects of protein and casein	1372:1404	The high correlation of fat with protein and casein contents suggests that the effect of fat on the cheese-making process is also attributable to the effects of protein and casein.
30146298	6	58	theme	goat	1062:1065	arg1	milk					1067:1070	goat milk	1062:1070	goat milk	1062:1070	The results allowed us to clarify the effect of the major milk nutrients on coagulation, curd firming, and syneresis ability of goat milk.
30146298	8	59	theme	casein	1399:1404	arg1	effects					1376:1382	the effects	1372:1382	the effects of protein and casein	1372:1404	The high correlation of fat with protein and casein contents suggests that the effect of fat on the cheese-making process is also attributable to the effects of protein and casein.
30146298	13	60	theme	milk	2229:2232	arg1	quality					2214:2220	the technological quality	2196:2220	the technological quality of the milk produced	2196:2241	These results are especially useful for dairy farmers who want to increase their profits by improving the technological quality of the milk produced.
30146298	12	61	theme	Higher	1867:1872	arg1	values					1874:1879	Higher values	1867:1879	Higher values of casein number	1867:1896	Higher values of casein number led to a general improvement in the coagulation ability of milk, suggesting that casein-to-protein ratio, not just protein or casein, should be considered when milk is destined for cheese making.
30146298	11	62	theme	strong	1771:1776	arg1	association					1778:1788	their strong association	1765:1788	their strong association	1765:1788	Consequently, given their strong association, we also tested the effect of casein-to-protein ratio (i.e., casein number).
30146298	7	63	dep	associated	1109:1118	arg1	whereas					1156:1162	whereas	1156:1162	whereas	1156:1162	In particular, milk rich in fat was associated with better coagulation properties, whereas milk rich in protein was associated with delayed coagulation.
30146298	13	64	theme	technological	2200:2212	arg1	quality					2214:2220	the technological quality	2196:2220	the technological quality of the milk produced	2196:2241	These results are especially useful for dairy farmers who want to increase their profits by improving the technological quality of the milk produced.
30146298	3	65	theme	coagulation	475:485	arg1	properties					487:496	Traditional milk coagulation properties	458:496	Traditional milk coagulation properties	458:496	Traditional milk coagulation properties and modeled curd-firming parameters were analyzed using a mixed model that considered the effect of days in milk, parity, farm, breed, the pendulum of the instrument, and different levels of fat, protein, and casein.
30146298	1	66	theme	casein	163:168	arg1	levels					135:140	different levels	125:140	different levels of fat, protein, and casein	125:168	The present study investigated the effect of different levels of fat, protein, and casein on (1) traditional milk coagulation properties, and (2) curd firming over time parameters of 1,272 goat milk samples.
30146298	6	67	theme	curd	1023:1026	arg1	firming					1028:1034	curd firming	1023:1034	curd firming	1023:1034	The results allowed us to clarify the effect of the major milk nutrients on coagulation, curd firming, and syneresis ability of goat milk.
30146298	10	68	theme	casein	1737:1742	arg1	role					1717:1720	the role	1713:1720	the role of protein and casein	1713:1742	The contemporary inclusion of protein and casein in the statistical model did not generate computing problems and allowed us to better characterize the role of protein and casein.
30146298	3	69	theme	Traditional	458:468	arg1	properties					487:496	Traditional milk coagulation properties	458:496	Traditional milk coagulation properties	458:496	Traditional milk coagulation properties and modeled curd-firming parameters were analyzed using a mixed model that considered the effect of days in milk, parity, farm, breed, the pendulum of the instrument, and different levels of fat, protein, and casein.
30146298	1	70	theme	present	84:90	arg1	study					92:96	The present study	80:96	The present study	80:96	The present study investigated the effect of different levels of fat, protein, and casein on (1) traditional milk coagulation properties, and (2) curd firming over time parameters of 1,272 goat milk samples.
30146298	8	71	theme	fat	1250:1252	arg1	correlation					1235:1245	The high correlation	1226:1245	The high correlation of fat with protein and casein contents	1226:1285	The high correlation of fat with protein and casein contents suggests that the effect of fat on the cheese-making process is also attributable to the effects of protein and casein.
30146298	10	72	theme	protein	1725:1731	arg1	role					1717:1720	the role	1713:1720	the role of protein and casein	1713:1742	The contemporary inclusion of protein and casein in the statistical model did not generate computing problems and allowed us to better characterize the role of protein and casein.
30146298	10	73	theme	protein	1595:1601	arg1	inclusion					1582:1590	The contemporary inclusion	1565:1590	The contemporary inclusion of protein and casein in the statistical model	1565:1637	The contemporary inclusion of protein and casein in the statistical model did not generate computing problems and allowed us to better characterize the role of protein and casein.
30146298	11	74	theme	casein-to-protein	1820:1836	arg1	ratio					1838:1842	casein-to-protein ratio	1820:1842	casein-to-protein ratio (i.e., casein number)	1820:1864	Consequently, given their strong association, we also tested the effect of casein-to-protein ratio (i.e., casein number).
30146298	8	75	theme	protein	1259:1265	arg1	contents					1278:1285	protein and casein contents	1259:1285	protein and casein contents	1259:1285	The high correlation of fat with protein and casein contents suggests that the effect of fat on the cheese-making process is also attributable to the effects of protein and casein.
30146298	7	76	theme	coagulation	1132:1142	arg1	properties					1144:1153	better coagulation properties	1125:1153	better coagulation properties	1125:1153	In particular, milk rich in fat was associated with better coagulation properties, whereas milk rich in protein was associated with delayed coagulation.
30146298	5	77	theme	component	862:870	arg1	effect					848:853	the effect	844:853	the effect of one component alone	844:876	Information provided by this model demonstrated the effect of one component alone, without contemporarily considering that of the others.
30146298	3	78	theme	protein	694:700	arg1	milk					606:609	milk	606:609	milk	606:609	Traditional milk coagulation properties and modeled curd-firming parameters were analyzed using a mixed model that considered the effect of days in milk, parity, farm, breed, the pendulum of the instrument, and different levels of fat, protein, and casein.
30146298	3	78	theme	protein	694:700	arg1	protein					694:700	protein	694:700	protein	694:700	Traditional milk coagulation properties and modeled curd-firming parameters were analyzed using a mixed model that considered the effect of days in milk, parity, farm, breed, the pendulum of the instrument, and different levels of fat, protein, and casein.
30146298	3	78	theme	protein	694:700	arg1	parity					612:617	parity	612:617	parity	612:617	Traditional milk coagulation properties and modeled curd-firming parameters were analyzed using a mixed model that considered the effect of days in milk, parity, farm, breed, the pendulum of the instrument, and different levels of fat, protein, and casein.
30146298	3	78	theme	protein	694:700	arg1	pendulum					637:644	the pendulum	633:644	the pendulum of the instrument	633:662	Traditional milk coagulation properties and modeled curd-firming parameters were analyzed using a mixed model that considered the effect of days in milk, parity, farm, breed, the pendulum of the instrument, and different levels of fat, protein, and casein.
30146298	3	78	theme	protein	694:700	arg1	levels					679:684	different levels	669:684	different levels of fat, protein, and casein	669:712	Traditional milk coagulation properties and modeled curd-firming parameters were analyzed using a mixed model that considered the effect of days in milk, parity, farm, breed, the pendulum of the instrument, and different levels of fat, protein, and casein.
30146298	3	78	theme	protein	694:700	arg1	farm					620:623	farm	620:623	farm	620:623	Traditional milk coagulation properties and modeled curd-firming parameters were analyzed using a mixed model that considered the effect of days in milk, parity, farm, breed, the pendulum of the instrument, and different levels of fat, protein, and casein.
30146298	3	78	theme	protein	694:700	arg1	casein					707:712	casein	707:712	casein	707:712	Traditional milk coagulation properties and modeled curd-firming parameters were analyzed using a mixed model that considered the effect of days in milk, parity, farm, breed, the pendulum of the instrument, and different levels of fat, protein, and casein.
30146298	3	78	theme	protein	694:700	arg1	fat					689:691	fat	689:691	fat	689:691	Traditional milk coagulation properties and modeled curd-firming parameters were analyzed using a mixed model that considered the effect of days in milk, parity, farm, breed, the pendulum of the instrument, and different levels of fat, protein, and casein.
30146298	3	78	theme	protein	694:700	arg1	breed					626:630	breed	626:630	breed	626:630	Traditional milk coagulation properties and modeled curd-firming parameters were analyzed using a mixed model that considered the effect of days in milk, parity, farm, breed, the pendulum of the instrument, and different levels of fat, protein, and casein.
30146298	3	78	theme	protein	694:700	arg1	instrument					653:662	the instrument	649:662	the instrument	649:662	Traditional milk coagulation properties and modeled curd-firming parameters were analyzed using a mixed model that considered the effect of days in milk, parity, farm, breed, the pendulum of the instrument, and different levels of fat, protein, and casein.
30146298	8	79	theme	casein	1271:1276	arg1	contents					1278:1285	protein and casein contents	1259:1285	protein and casein contents	1259:1285	The high correlation of fat with protein and casein contents suggests that the effect of fat on the cheese-making process is also attributable to the effects of protein and casein.
30146298	6	80	theme	major	986:990	arg1	nutrients					997:1005	the major milk nutrients	982:1005	the major milk nutrients	982:1005	The results allowed us to clarify the effect of the major milk nutrients on coagulation, curd firming, and syneresis ability of goat milk.
30146298	3	81	theme	instrument	653:662	arg1	milk					606:609	milk	606:609	milk	606:609	Traditional milk coagulation properties and modeled curd-firming parameters were analyzed using a mixed model that considered the effect of days in milk, parity, farm, breed, the pendulum of the instrument, and different levels of fat, protein, and casein.
30146298	3	81	theme	instrument	653:662	arg1	protein					694:700	protein	694:700	protein	694:700	Traditional milk coagulation properties and modeled curd-firming parameters were analyzed using a mixed model that considered the effect of days in milk, parity, farm, breed, the pendulum of the instrument, and different levels of fat, protein, and casein.
30146298	3	81	theme	instrument	653:662	arg1	parity					612:617	parity	612:617	parity	612:617	Traditional milk coagulation properties and modeled curd-firming parameters were analyzed using a mixed model that considered the effect of days in milk, parity, farm, breed, the pendulum of the instrument, and different levels of fat, protein, and casein.
30146298	3	81	theme	instrument	653:662	arg1	pendulum					637:644	the pendulum	633:644	the pendulum of the instrument	633:662	Traditional milk coagulation properties and modeled curd-firming parameters were analyzed using a mixed model that considered the effect of days in milk, parity, farm, breed, the pendulum of the instrument, and different levels of fat, protein, and casein.
30146298	3	81	theme	instrument	653:662	arg1	levels					679:684	different levels	669:684	different levels of fat, protein, and casein	669:712	Traditional milk coagulation properties and modeled curd-firming parameters were analyzed using a mixed model that considered the effect of days in milk, parity, farm, breed, the pendulum of the instrument, and different levels of fat, protein, and casein.
30146298	3	81	theme	instrument	653:662	arg1	farm					620:623	farm	620:623	farm	620:623	Traditional milk coagulation properties and modeled curd-firming parameters were analyzed using a mixed model that considered the effect of days in milk, parity, farm, breed, the pendulum of the instrument, and different levels of fat, protein, and casein.
30146298	3	81	theme	instrument	653:662	arg1	casein					707:712	casein	707:712	casein	707:712	Traditional milk coagulation properties and modeled curd-firming parameters were analyzed using a mixed model that considered the effect of days in milk, parity, farm, breed, the pendulum of the instrument, and different levels of fat, protein, and casein.
30146298	3	81	theme	instrument	653:662	arg1	fat					689:691	fat	689:691	fat	689:691	Traditional milk coagulation properties and modeled curd-firming parameters were analyzed using a mixed model that considered the effect of days in milk, parity, farm, breed, the pendulum of the instrument, and different levels of fat, protein, and casein.
30146298	3	81	theme	instrument	653:662	arg1	breed					626:630	breed	626:630	breed	626:630	Traditional milk coagulation properties and modeled curd-firming parameters were analyzed using a mixed model that considered the effect of days in milk, parity, farm, breed, the pendulum of the instrument, and different levels of fat, protein, and casein.
30146298	3	81	theme	instrument	653:662	arg1	instrument					653:662	the instrument	649:662	the instrument	649:662	Traditional milk coagulation properties and modeled curd-firming parameters were analyzed using a mixed model that considered the effect of days in milk, parity, farm, breed, the pendulum of the instrument, and different levels of fat, protein, and casein.
30146298	2	82	dep	status	371:376	arg1	NaCl					428:431	NaCl	428:431	NaCl	428:431	Relationships between fat, protein, and casein and some indicators of udder health status (lactose, pH, somatic cells, bacterial count, and NaCl) were also investigated.
30146298	2	82	dep	status	371:376	arg1	count					417:421	bacterial count	407:421	bacterial count	407:421	Relationships between fat, protein, and casein and some indicators of udder health status (lactose, pH, somatic cells, bacterial count, and NaCl) were also investigated.
30146298	2	82	dep	status	371:376	arg1	pH					388:389	pH	388:389	pH	388:389	Relationships between fat, protein, and casein and some indicators of udder health status (lactose, pH, somatic cells, bacterial count, and NaCl) were also investigated.
30146298	2	82	dep	status	371:376	arg1	cells					400:404	somatic cells	392:404	somatic cells	392:404	Relationships between fat, protein, and casein and some indicators of udder health status (lactose, pH, somatic cells, bacterial count, and NaCl) were also investigated.
30146298	2	82	dep	status	371:376	arg1	lactose					379:385	lactose	379:385	lactose	379:385	Relationships between fat, protein, and casein and some indicators of udder health status (lactose, pH, somatic cells, bacterial count, and NaCl) were also investigated.
30146298	1	83	theme	different	125:133	arg1	levels					135:140	different levels	125:140	different levels of fat, protein, and casein	125:168	The present study investigated the effect of different levels of fat, protein, and casein on (1) traditional milk coagulation properties, and (2) curd firming over time parameters of 1,272 goat milk samples.
30146298	9	84	theme	firming	1512:1518	arg1	pattern					1483:1489	the pattern	1479:1489	the pattern of coagulation, curd firming, and syneresis	1479:1533	When only protein or only casein was included in the statistical model, the pattern of coagulation, curd firming, and syneresis was almost indistinguishable.
30146298	9	84	theme	firming	1512:1518	arg1	indistinguishable					1546:1562	indistinguishable	1546:1562	indistinguishable	1546:1562	When only protein or only casein was included in the statistical model, the pattern of coagulation, curd firming, and syneresis was almost indistinguishable.
30146298	3	85	theme	fat	689:691	arg1	milk					606:609	milk	606:609	milk	606:609	Traditional milk coagulation properties and modeled curd-firming parameters were analyzed using a mixed model that considered the effect of days in milk, parity, farm, breed, the pendulum of the instrument, and different levels of fat, protein, and casein.
30146298	3	85	theme	fat	689:691	arg1	protein					694:700	protein	694:700	protein	694:700	Traditional milk coagulation properties and modeled curd-firming parameters were analyzed using a mixed model that considered the effect of days in milk, parity, farm, breed, the pendulum of the instrument, and different levels of fat, protein, and casein.
30146298	3	85	theme	fat	689:691	arg1	parity					612:617	parity	612:617	parity	612:617	Traditional milk coagulation properties and modeled curd-firming parameters were analyzed using a mixed model that considered the effect of days in milk, parity, farm, breed, the pendulum of the instrument, and different levels of fat, protein, and casein.
30146298	3	85	theme	fat	689:691	arg1	pendulum					637:644	the pendulum	633:644	the pendulum of the instrument	633:662	Traditional milk coagulation properties and modeled curd-firming parameters were analyzed using a mixed model that considered the effect of days in milk, parity, farm, breed, the pendulum of the instrument, and different levels of fat, protein, and casein.
30146298	3	85	theme	fat	689:691	arg1	levels					679:684	different levels	669:684	different levels of fat, protein, and casein	669:712	Traditional milk coagulation properties and modeled curd-firming parameters were analyzed using a mixed model that considered the effect of days in milk, parity, farm, breed, the pendulum of the instrument, and different levels of fat, protein, and casein.
30146298	3	85	theme	fat	689:691	arg1	farm					620:623	farm	620:623	farm	620:623	Traditional milk coagulation properties and modeled curd-firming parameters were analyzed using a mixed model that considered the effect of days in milk, parity, farm, breed, the pendulum of the instrument, and different levels of fat, protein, and casein.
30146298	3	85	theme	fat	689:691	arg1	casein					707:712	casein	707:712	casein	707:712	Traditional milk coagulation properties and modeled curd-firming parameters were analyzed using a mixed model that considered the effect of days in milk, parity, farm, breed, the pendulum of the instrument, and different levels of fat, protein, and casein.
30146298	3	85	theme	fat	689:691	arg1	fat					689:691	fat	689:691	fat	689:691	Traditional milk coagulation properties and modeled curd-firming parameters were analyzed using a mixed model that considered the effect of days in milk, parity, farm, breed, the pendulum of the instrument, and different levels of fat, protein, and casein.
30146298	3	85	theme	fat	689:691	arg1	breed					626:630	breed	626:630	breed	626:630	Traditional milk coagulation properties and modeled curd-firming parameters were analyzed using a mixed model that considered the effect of days in milk, parity, farm, breed, the pendulum of the instrument, and different levels of fat, protein, and casein.
30146298	3	85	theme	fat	689:691	arg1	instrument					653:662	the instrument	649:662	the instrument	649:662	Traditional milk coagulation properties and modeled curd-firming parameters were analyzed using a mixed model that considered the effect of days in milk, parity, farm, breed, the pendulum of the instrument, and different levels of fat, protein, and casein.
30146298	0	86	theme	milk	74:77	arg1	syneresis					56:64	syneresis	56:64	syneresis of goat milk	56:77	Effect of composition on coagulation, curd firming, and syneresis of goat milk.
30146298	0	86	theme	milk	74:77	arg1	coagulation					25:35	coagulation	25:35	coagulation	25:35	Effect of composition on coagulation, curd firming, and syneresis of goat milk.
30146298	0	86	theme	milk	74:77	arg1	firming					43:49	curd firming	38:49	curd firming	38:49	Effect of composition on coagulation, curd firming, and syneresis of goat milk.
30146298	1	87	theme	goat	269:272	arg1	samples					279:285	1,272 goat milk samples	263:285	1,272 goat milk samples	263:285	The present study investigated the effect of different levels of fat, protein, and casein on (1) traditional milk coagulation properties, and (2) curd firming over time parameters of 1,272 goat milk samples.
30146298	2	88	theme	bacterial	407:415	arg1	count					417:421	bacterial count	407:421	bacterial count	407:421	Relationships between fat, protein, and casein and some indicators of udder health status (lactose, pH, somatic cells, bacterial count, and NaCl) were also investigated.
30146298	12	89	theme	casein-to-protein	1979:1995	arg1	ratio					1997:2001	casein-to-protein ratio	1979:2001	casein-to-protein ratio	1979:2001	Higher values of casein number led to a general improvement in the coagulation ability of milk, suggesting that casein-to-protein ratio, not just protein or casein, should be considered when milk is destined for cheese making.
30146298	6	90	from	effect	972:977	arg1	ability					1051:1057	syneresis ability	1041:1057	syneresis ability	1041:1057	The results allowed us to clarify the effect of the major milk nutrients on coagulation, curd firming, and syneresis ability of goat milk.
30146298	6	90	from	effect	972:977	arg1	coagulation					1010:1020	coagulation	1010:1020	coagulation	1010:1020	The results allowed us to clarify the effect of the major milk nutrients on coagulation, curd firming, and syneresis ability of goat milk.
30146298	6	90	from	effect	972:977	arg1	firming					1028:1034	curd firming	1023:1034	curd firming	1023:1034	The results allowed us to clarify the effect of the major milk nutrients on coagulation, curd firming, and syneresis ability of goat milk.
30146298	1	91	theme	samples	279:285	arg1	parameters					249:258	time parameters	244:258	time parameters of 1,272 goat milk samples	244:285	The present study investigated the effect of different levels of fat, protein, and casein on (1) traditional milk coagulation properties, and (2) curd firming over time parameters of 1,272 goat milk samples.
30146298	7	92	theme	rich	1093:1096	arg1	milk					1088:1091	milk	1088:1091	milk rich in fat	1088:1103	In particular, milk rich in fat was associated with better coagulation properties, whereas milk rich in protein was associated with delayed coagulation.
30146298	12	93	dep	ratio	1997:2001	arg1	just					2008:2011	just	2008:2011	just	2008:2011	Higher values of casein number led to a general improvement in the coagulation ability of milk, suggesting that casein-to-protein ratio, not just protein or casein, should be considered when milk is destined for cheese making.
30146298	8	94	theme	cheese-making	1326:1338	arg1	process					1340:1346	the cheese-making process	1322:1346	the cheese-making process	1322:1346	The high correlation of fat with protein and casein contents suggests that the effect of fat on the cheese-making process is also attributable to the effects of protein and casein.
30146298	12	95	theme	coagulation	1934:1944	arg1	ability					1946:1952	the coagulation ability	1930:1952	the coagulation ability of milk	1930:1960	Higher values of casein number led to a general improvement in the coagulation ability of milk, suggesting that casein-to-protein ratio, not just protein or casein, should be considered when milk is destined for cheese making.
30146298	10	96	theme	casein	1607:1612	arg1	inclusion					1582:1590	The contemporary inclusion	1565:1590	The contemporary inclusion of protein and casein in the statistical model	1565:1637	The contemporary inclusion of protein and casein in the statistical model did not generate computing problems and allowed us to better characterize the role of protein and casein.
30146298	2	97	theme	health	364:369	arg1	status					371:376	udder health status	358:376	udder health status (lactose, pH, somatic cells, bacterial count, and NaCl)	358:432	Relationships between fat, protein, and casein and some indicators of udder health status (lactose, pH, somatic cells, bacterial count, and NaCl) were also investigated.
30146298	12	98	theme	general	1907:1913	arg1	improvement					1915:1925	a general improvement	1905:1925	a general improvement in the coagulation ability of milk	1905:1960	Higher values of casein number led to a general improvement in the coagulation ability of milk, suggesting that casein-to-protein ratio, not just protein or casein, should be considered when milk is destined for cheese making.
30146298	12	99	from	improvement	1915:1925	arg1	ability					1946:1952	the coagulation ability	1930:1952	the coagulation ability of milk	1930:1960	Higher values of casein number led to a general improvement in the coagulation ability of milk, suggesting that casein-to-protein ratio, not just protein or casein, should be considered when milk is destined for cheese making.
30146298	1	100	theme	time	244:247	arg1	parameters					249:258	time parameters	244:258	time parameters of 1,272 goat milk samples	244:285	The present study investigated the effect of different levels of fat, protein, and casein on (1) traditional milk coagulation properties, and (2) curd firming over time parameters of 1,272 goat milk samples.
30146298	3	101	theme	mixed	556:560	arg1	model					562:566	a mixed model	554:566	a mixed model that considered the effect of days in milk, parity, farm, breed, the pendulum of the instrument, and different levels of fat, protein, and casein	554:712	Traditional milk coagulation properties and modeled curd-firming parameters were analyzed using a mixed model that considered the effect of days in milk, parity, farm, breed, the pendulum of the instrument, and different levels of fat, protein, and casein.
30146298	12	102	theme	number	1891:1896	arg1	values					1874:1879	Higher values	1867:1879	Higher values of casein number	1867:1896	Higher values of casein number led to a general improvement in the coagulation ability of milk, suggesting that casein-to-protein ratio, not just protein or casein, should be considered when milk is destined for cheese making.
30146298	1	103	theme	traditional	177:187	arg1	properties					206:215	(1) traditional milk coagulation properties	173:215	(1) traditional milk coagulation properties	173:215	The present study investigated the effect of different levels of fat, protein, and casein on (1) traditional milk coagulation properties, and (2) curd firming over time parameters of 1,272 goat milk samples.
30370555	9	0	theme	AMPARs	1367:1372	arg1	diversity					1348:1356	the molecular and functional diversity	1319:1356	the molecular and functional diversity of glial AMPARs	1319:1372	Our study provides a workflow and a first reference for future investigations into the molecular and functional diversity of glial AMPARs.
30370555	7	1	theme	minor	986:990	arg1	variation					992:1000	minor variation	986:1000	minor variation	986:1000	Whereas the early postnatal pattern of AMPAR mRNA expression showed minor variation over time, it did show significant heterogeneity in different brain regions.
30370555	6	2	theme	selected	894:901	arg1	regions					909:915	selected brain regions	894:915	selected brain regions	894:915	In this study, we sought to quantify the astrocytic AMPAR transcriptome during brain development and with respect to selected brain regions.
30370555	4	3	theme	core	553:556	arg1	constituents					581:592	peripheral complex constituents	562:592	peripheral complex constituents	562:592	AMPARs form as macromolecular protein complexes, whose composition of the pore-lining GluA subunits and of an extensive set of core and peripheral complex constituents defines both their trafficking and gating behavior.
30370555	4	3	theme	core	553:556	arg1	core					553:556	core	553:556	core	553:556	AMPARs form as macromolecular protein complexes, whose composition of the pore-lining GluA subunits and of an extensive set of core and peripheral complex constituents defines both their trafficking and gating behavior.
30370555	4	3	theme	core	553:556	arg1	set					546:548	an extensive set	533:548	an extensive set of core and peripheral complex constituents	533:592	AMPARs form as macromolecular protein complexes, whose composition of the pore-lining GluA subunits and of an extensive set of core and peripheral complex constituents defines both their trafficking and gating behavior.
30370555	8	4	theme	Cerebellar	1079:1088	arg1	astrocytes					1090:1099	Cerebellar astrocytes	1079:1099	Cerebellar astrocytes	1079:1099	Cerebellar astrocytes express a combination of AMPAR complex constituents that is remarkably distinct from the one in neocortical or hippocampal astrocytes.
30370555	6	5	theme	astrocytic	818:827	arg1	transcriptome					835:847	the astrocytic AMPAR transcriptome	814:847	the astrocytic AMPAR transcriptome	814:847	In this study, we sought to quantify the astrocytic AMPAR transcriptome during brain development and with respect to selected brain regions.
30370555	7	6	theme	mRNA	963:966	arg1	expression					968:977	AMPAR mRNA expression	957:977	AMPAR mRNA expression	957:977	Whereas the early postnatal pattern of AMPAR mRNA expression showed minor variation over time, it did show significant heterogeneity in different brain regions.
30370555	6	7	theme	brain	856:860	arg1	development					862:872	brain development	856:872	brain development	856:872	In this study, we sought to quantify the astrocytic AMPAR transcriptome during brain development and with respect to selected brain regions.
30370555	3	8	theme	receptors	351:359	arg1	expression					316:325	the expression	312:325	the expression of ionotropic glutamate receptors of the AMPA subtype (AMPARs) in astrocytes	312:402	For many of them, the expression of ionotropic glutamate receptors of the AMPA subtype (AMPARs) in astrocytes is of key importance.
30370555	6	9	theme	brain	903:907	arg1	regions					909:915	selected brain regions	894:915	selected brain regions	894:915	In this study, we sought to quantify the astrocytic AMPAR transcriptome during brain development and with respect to selected brain regions.
30370555	7	10	theme	expression	968:977	arg1	pattern					946:952	the early postnatal pattern	926:952	the early postnatal pattern of AMPAR mRNA expression	926:977	Whereas the early postnatal pattern of AMPAR mRNA expression showed minor variation over time, it did show significant heterogeneity in different brain regions.
30370555	4	11	theme	peripheral	562:571	arg1	constituents					581:592	peripheral complex constituents	562:592	peripheral complex constituents	562:592	AMPARs form as macromolecular protein complexes, whose composition of the pore-lining GluA subunits and of an extensive set of core and peripheral complex constituents defines both their trafficking and gating behavior.
30370555	4	12	theme	protein	456:462	arg1	complexes					464:472	macromolecular protein complexes	441:472	macromolecular protein complexes	441:472	AMPARs form as macromolecular protein complexes, whose composition of the pore-lining GluA subunits and of an extensive set of core and peripheral complex constituents defines both their trafficking and gating behavior.
30370555	5	13	theme	astrocytic	655:664	arg1	AMPARs					666:671	astrocytic AMPARs	655:671	astrocytic AMPARs	655:671	Although astrocytic AMPARs have been reported to exhibit heterogeneous properties, their molecular composition is largely unknown.
30370555	8	14	theme	hippocampal	1212:1222	arg1	astrocytes					1224:1233	neocortical or hippocampal astrocytes	1197:1233	neocortical or hippocampal astrocytes	1197:1233	Cerebellar astrocytes express a combination of AMPAR complex constituents that is remarkably distinct from the one in neocortical or hippocampal astrocytes.
30370555	3	15	theme	ionotropic	330:339	arg1	receptors					351:359	ionotropic glutamate receptors	330:359	ionotropic glutamate receptors of the AMPA subtype (AMPARs)	330:388	For many of them, the expression of ionotropic glutamate receptors of the AMPA subtype (AMPARs) in astrocytes is of key importance.
30370555	3	15	theme	ionotropic	330:339	arg1	AMPARs					382:387	AMPARs	382:387	AMPARs	382:387	For many of them, the expression of ionotropic glutamate receptors of the AMPA subtype (AMPARs) in astrocytes is of key importance.
30370555	4	16	theme	macromolecular	441:454	arg1	complexes					464:472	macromolecular protein complexes	441:472	macromolecular protein complexes	441:472	AMPARs form as macromolecular protein complexes, whose composition of the pore-lining GluA subunits and of an extensive set of core and peripheral complex constituents defines both their trafficking and gating behavior.
30370555	7	17	theme	AMPAR	957:961	arg1	expression					968:977	AMPAR mRNA expression	957:977	AMPAR mRNA expression	957:977	Whereas the early postnatal pattern of AMPAR mRNA expression showed minor variation over time, it did show significant heterogeneity in different brain regions.
30370555	1	18	theme	largest	81:87	arg1	class					89:93	the largest class	77:93	the largest class of glial cells	77:108	Astrocytes form the largest class of glial cells in the central nervous system.
30370555	3	19	theme	key	410:412	arg1	importance					414:423	key importance	410:423	key importance	410:423	For many of them, the expression of ionotropic glutamate receptors of the AMPA subtype (AMPARs) in astrocytes is of key importance.
30370555	3	20	theme	glutamate	341:349	arg1	receptors					351:359	ionotropic glutamate receptors	330:359	ionotropic glutamate receptors of the AMPA subtype (AMPARs)	330:388	For many of them, the expression of ionotropic glutamate receptors of the AMPA subtype (AMPARs) in astrocytes is of key importance.
30370555	3	20	theme	glutamate	341:349	arg1	AMPARs					382:387	AMPARs	382:387	AMPARs	382:387	For many of them, the expression of ionotropic glutamate receptors of the AMPA subtype (AMPARs) in astrocytes is of key importance.
30370555	9	21	theme	functional	1337:1346	arg1	diversity					1348:1356	the molecular and functional diversity	1319:1356	the molecular and functional diversity of glial AMPARs	1319:1372	Our study provides a workflow and a first reference for future investigations into the molecular and functional diversity of glial AMPARs.
30370555	7	22	theme	postnatal	936:944	arg1	pattern					946:952	the early postnatal pattern	926:952	the early postnatal pattern of AMPAR mRNA expression	926:977	Whereas the early postnatal pattern of AMPAR mRNA expression showed minor variation over time, it did show significant heterogeneity in different brain regions.
30370555	5	23	theme	molecular	735:743	arg1	composition					745:755	their molecular composition	729:755	their molecular composition	729:755	Although astrocytic AMPARs have been reported to exhibit heterogeneous properties, their molecular composition is largely unknown.
30370555	3	24	theme	subtype	373:379	arg1	receptors					351:359	ionotropic glutamate receptors	330:359	ionotropic glutamate receptors of the AMPA subtype (AMPARs)	330:388	For many of them, the expression of ionotropic glutamate receptors of the AMPA subtype (AMPARs) in astrocytes is of key importance.
30370555	3	24	theme	subtype	373:379	arg1	AMPARs					382:387	AMPARs	382:387	AMPARs	382:387	For many of them, the expression of ionotropic glutamate receptors of the AMPA subtype (AMPARs) in astrocytes is of key importance.
30370555	9	25	theme	molecular	1323:1331	arg1	diversity					1348:1356	the molecular and functional diversity	1319:1356	the molecular and functional diversity of glial AMPARs	1319:1372	Our study provides a workflow and a first reference for future investigations into the molecular and functional diversity of glial AMPARs.
30370555	7	26	theme	different	1054:1062	arg1	regions					1070:1076	different brain regions	1054:1076	different brain regions	1054:1076	Whereas the early postnatal pattern of AMPAR mRNA expression showed minor variation over time, it did show significant heterogeneity in different brain regions.
30370555	1	27	theme	glial	98:102	arg1	cells					104:108	glial cells	98:108	glial cells	98:108	Astrocytes form the largest class of glial cells in the central nervous system.
30370555	0	28	theme	astrocytic	21:30	arg1	transcriptome					46:58	the astrocytic AMPA-receptor transcriptome	17:58	the astrocytic AMPA-receptor transcriptome	17:58	Heterogeneity of the astrocytic AMPA-receptor transcriptome.
30370555	9	29	theme	glial	1361:1365	arg1	AMPARs					1367:1372	glial AMPARs	1361:1372	glial AMPARs	1361:1372	Our study provides a workflow and a first reference for future investigations into the molecular and functional diversity of glial AMPARs.
30370555	7	30	theme	early	930:934	arg1	pattern					946:952	the early postnatal pattern	926:952	the early postnatal pattern of AMPAR mRNA expression	926:977	Whereas the early postnatal pattern of AMPAR mRNA expression showed minor variation over time, it did show significant heterogeneity in different brain regions.
30370555	1	31	theme	cells	104:108	arg1	class					89:93	the largest class	77:93	the largest class of glial cells	77:108	Astrocytes form the largest class of glial cells in the central nervous system.
30370555	3	32	theme	AMPA	368:371	arg1	subtype					373:379	the AMPA subtype	364:379	the AMPA subtype	364:379	For many of them, the expression of ionotropic glutamate receptors of the AMPA subtype (AMPARs) in astrocytes is of key importance.
30370555	4	33	theme	set	546:548	arg1	composition					481:491	composition	481:491	composition	481:491	AMPARs form as macromolecular protein complexes, whose composition of the pore-lining GluA subunits and of an extensive set of core and peripheral complex constituents defines both their trafficking and gating behavior.
30370555	2	34	theme	blood-brain	273:283	arg1	barrier					285:291	the blood-brain barrier	269:291	the blood-brain barrier	269:291	They serve plenty of diverse functions that range from supporting the formation and proper operation of synapses to controlling the blood-brain barrier.
30370555	2	35	dep	formation	211:219	arg1	the					207:209	the	207:209	the	207:209	They serve plenty of diverse functions that range from supporting the formation and proper operation of synapses to controlling the blood-brain barrier.
30370555	0	36	theme	transcriptome	46:58	arg1	Heterogeneity					0:12	Heterogeneity	0:12	Heterogeneity of the astrocytic AMPA-receptor transcriptome	0:58	Heterogeneity of the astrocytic AMPA-receptor transcriptome.
30370555	4	37	theme	extensive	536:544	arg1	constituents					581:592	peripheral complex constituents	562:592	peripheral complex constituents	562:592	AMPARs form as macromolecular protein complexes, whose composition of the pore-lining GluA subunits and of an extensive set of core and peripheral complex constituents defines both their trafficking and gating behavior.
30370555	4	37	theme	extensive	536:544	arg1	core					553:556	core	553:556	core	553:556	AMPARs form as macromolecular protein complexes, whose composition of the pore-lining GluA subunits and of an extensive set of core and peripheral complex constituents defines both their trafficking and gating behavior.
30370555	4	37	theme	extensive	536:544	arg1	set					546:548	an extensive set	533:548	an extensive set of core and peripheral complex constituents	533:592	AMPARs form as macromolecular protein complexes, whose composition of the pore-lining GluA subunits and of an extensive set of core and peripheral complex constituents defines both their trafficking and gating behavior.
30370555	5	38	theme	heterogeneous	703:715	arg1	properties					717:726	heterogeneous properties	703:726	heterogeneous properties	703:726	Although astrocytic AMPARs have been reported to exhibit heterogeneous properties, their molecular composition is largely unknown.
30370555	2	39	theme	functions	170:178	arg1	plenty					152:157	plenty	152:157	plenty of diverse functions that range	152:189	They serve plenty of diverse functions that range from supporting the formation and proper operation of synapses to controlling the blood-brain barrier.
30370555	0	40	gly	Heterogeneity	0:12	arg1	transcriptome					46:58	the astrocytic AMPA-receptor transcriptome	17:58	the astrocytic AMPA-receptor transcriptome	17:58	Heterogeneity of the astrocytic AMPA-receptor transcriptome.
30370555	4	41	theme	gating	629:634	arg1	behavior					636:643	gating behavior	629:643	gating behavior	629:643	AMPARs form as macromolecular protein complexes, whose composition of the pore-lining GluA subunits and of an extensive set of core and peripheral complex constituents defines both their trafficking and gating behavior.
30370555	0	42	theme	AMPA-receptor	32:44	arg1	transcriptome					46:58	the astrocytic AMPA-receptor transcriptome	17:58	the astrocytic AMPA-receptor transcriptome	17:58	Heterogeneity of the astrocytic AMPA-receptor transcriptome.
30370555	4	43	theme	GluA	512:515	arg1	subunits					517:524	the pore-lining GluA subunits	496:524	the pore-lining GluA subunits	496:524	AMPARs form as macromolecular protein complexes, whose composition of the pore-lining GluA subunits and of an extensive set of core and peripheral complex constituents defines both their trafficking and gating behavior.
30370555	7	44	theme	brain	1064:1068	arg1	regions					1070:1076	different brain regions	1054:1076	different brain regions	1054:1076	Whereas the early postnatal pattern of AMPAR mRNA expression showed minor variation over time, it did show significant heterogeneity in different brain regions.
30370555	2	45	theme	diverse	162:168	arg1	functions					170:178	diverse functions	162:178	diverse functions	162:178	They serve plenty of diverse functions that range from supporting the formation and proper operation of synapses to controlling the blood-brain barrier.
30370555	2	46	theme	proper	225:230	arg1	operation					232:240	proper operation	225:240	proper operation	225:240	They serve plenty of diverse functions that range from supporting the formation and proper operation of synapses to controlling the blood-brain barrier.
30370555	4	47	theme	pore-lining	500:510	arg1	subunits					517:524	the pore-lining GluA subunits	496:524	the pore-lining GluA subunits	496:524	AMPARs form as macromolecular protein complexes, whose composition of the pore-lining GluA subunits and of an extensive set of core and peripheral complex constituents defines both their trafficking and gating behavior.
30370555	3	48	theme	them	306:309	arg1	many					298:301	many	298:301	many	298:301	For many of them, the expression of ionotropic glutamate receptors of the AMPA subtype (AMPARs) in astrocytes is of key importance.
30370555	3	48	theme	them	306:309	arg1	them					306:309	them	306:309	them	306:309	For many of them, the expression of ionotropic glutamate receptors of the AMPA subtype (AMPARs) in astrocytes is of key importance.
30370555	8	49	from	one	1190:1192	arg1	combination					1111:1121	a combination	1109:1121	a combination of AMPAR complex constituents that is remarkably distinct from the one in neocortical or hippocampal astrocytes	1109:1233	Cerebellar astrocytes express a combination of AMPAR complex constituents that is remarkably distinct from the one in neocortical or hippocampal astrocytes.
30370555	8	49	from	one	1190:1192	arg1	distinct					1172:1179	distinct	1172:1179	distinct	1172:1179	Cerebellar astrocytes express a combination of AMPAR complex constituents that is remarkably distinct from the one in neocortical or hippocampal astrocytes.
30370555	8	49	from	one	1190:1192	arg1	astrocytes					1224:1233	neocortical or hippocampal astrocytes	1197:1233	neocortical or hippocampal astrocytes	1197:1233	Cerebellar astrocytes express a combination of AMPAR complex constituents that is remarkably distinct from the one in neocortical or hippocampal astrocytes.
30370555	9	50	theme	first	1272:1276	arg1	reference					1278:1286	a first reference	1270:1286	a first reference for future investigations into the molecular and functional diversity of glial AMPARs	1270:1372	Our study provides a workflow and a first reference for future investigations into the molecular and functional diversity of glial AMPARs.
30370555	1	51	theme	central	117:123	arg1	system					133:138	the central nervous system	113:138	the central nervous system	113:138	Astrocytes form the largest class of glial cells in the central nervous system.
30370555	4	52	theme	subunits	517:524	arg1	composition					481:491	composition	481:491	composition	481:491	AMPARs form as macromolecular protein complexes, whose composition of the pore-lining GluA subunits and of an extensive set of core and peripheral complex constituents defines both their trafficking and gating behavior.
30370555	8	53	theme	complex	1132:1138	arg1	constituents					1140:1151	AMPAR complex constituents	1126:1151	AMPAR complex constituents	1126:1151	Cerebellar astrocytes express a combination of AMPAR complex constituents that is remarkably distinct from the one in neocortical or hippocampal astrocytes.
30370555	6	54	theme	AMPAR	829:833	arg1	transcriptome					835:847	the astrocytic AMPAR transcriptome	814:847	the astrocytic AMPAR transcriptome	814:847	In this study, we sought to quantify the astrocytic AMPAR transcriptome during brain development and with respect to selected brain regions.
30370555	8	55	theme	AMPAR	1126:1130	arg1	constituents					1140:1151	AMPAR complex constituents	1126:1151	AMPAR complex constituents	1126:1151	Cerebellar astrocytes express a combination of AMPAR complex constituents that is remarkably distinct from the one in neocortical or hippocampal astrocytes.
30370555	1	56	theme	nervous	125:131	arg1	system					133:138	the central nervous system	113:138	the central nervous system	113:138	Astrocytes form the largest class of glial cells in the central nervous system.
30370555	4	57	theme	constituents	581:592	arg1	constituents					581:592	peripheral complex constituents	562:592	peripheral complex constituents	562:592	AMPARs form as macromolecular protein complexes, whose composition of the pore-lining GluA subunits and of an extensive set of core and peripheral complex constituents defines both their trafficking and gating behavior.
30370555	4	57	theme	constituents	581:592	arg1	core					553:556	core	553:556	core	553:556	AMPARs form as macromolecular protein complexes, whose composition of the pore-lining GluA subunits and of an extensive set of core and peripheral complex constituents defines both their trafficking and gating behavior.
30370555	4	57	theme	constituents	581:592	arg1	set					546:548	an extensive set	533:548	an extensive set of core and peripheral complex constituents	533:592	AMPARs form as macromolecular protein complexes, whose composition of the pore-lining GluA subunits and of an extensive set of core and peripheral complex constituents defines both their trafficking and gating behavior.
30370555	7	58	theme	significant	1025:1035	arg1	heterogeneity					1037:1049	significant heterogeneity	1025:1049	significant heterogeneity in different brain regions	1025:1076	Whereas the early postnatal pattern of AMPAR mRNA expression showed minor variation over time, it did show significant heterogeneity in different brain regions.
30370555	3	59	from	expression	316:325	arg1	astrocytes					393:402	astrocytes	393:402	astrocytes	393:402	For many of them, the expression of ionotropic glutamate receptors of the AMPA subtype (AMPARs) in astrocytes is of key importance.
30370555	4	60	theme	complex	573:579	arg1	constituents					581:592	peripheral complex constituents	562:592	peripheral complex constituents	562:592	AMPARs form as macromolecular protein complexes, whose composition of the pore-lining GluA subunits and of an extensive set of core and peripheral complex constituents defines both their trafficking and gating behavior.
30370555	2	61	theme	synapses	245:252	arg1	formation					211:219	formation	211:219	formation	211:219	They serve plenty of diverse functions that range from supporting the formation and proper operation of synapses to controlling the blood-brain barrier.
30370555	2	61	theme	synapses	245:252	arg1	operation					232:240	proper operation	225:240	proper operation	225:240	They serve plenty of diverse functions that range from supporting the formation and proper operation of synapses to controlling the blood-brain barrier.
30370555	7	62	from	heterogeneity	1037:1049	arg1	regions					1070:1076	different brain regions	1054:1076	different brain regions	1054:1076	Whereas the early postnatal pattern of AMPAR mRNA expression showed minor variation over time, it did show significant heterogeneity in different brain regions.
30370555	8	63	theme	constituents	1140:1151	arg1	distinct					1172:1179	distinct	1172:1179	distinct	1172:1179	Cerebellar astrocytes express a combination of AMPAR complex constituents that is remarkably distinct from the one in neocortical or hippocampal astrocytes.
30370555	8	63	theme	constituents	1140:1151	arg1	combination					1111:1121	a combination	1109:1121	a combination of AMPAR complex constituents that is remarkably distinct from the one in neocortical or hippocampal astrocytes	1109:1233	Cerebellar astrocytes express a combination of AMPAR complex constituents that is remarkably distinct from the one in neocortical or hippocampal astrocytes.
30370555	9	64	theme	future	1292:1297	arg1	investigations					1299:1312	future investigations	1292:1312	future investigations into the molecular and functional diversity of glial AMPARs	1292:1372	Our study provides a workflow and a first reference for future investigations into the molecular and functional diversity of glial AMPARs.
30370555	8	65	theme	neocortical	1197:1207	arg1	astrocytes					1224:1233	neocortical or hippocampal astrocytes	1197:1233	neocortical or hippocampal astrocytes	1197:1233	Cerebellar astrocytes express a combination of AMPAR complex constituents that is remarkably distinct from the one in neocortical or hippocampal astrocytes.
35021414	7	0	theme	composition	1097:1107	arg1	contents					1109:1116	composition contents	1097:1116	composition contents	1097:1116	We demonstrated that the copolymer sequences and composition contents made a difference to the properties of the formed hydrogels by variation of the cross-linking density.
35021414	6	1	theme	Sar-co-GH	1023:1031	arg1	hydrogel					1038:1045	the P(Sar-co-GH)-OSA hydrogel	1017:1045	the P(Sar-co-GH)-OSA hydrogel	1017:1045	Moreover, cellulose nanocrystals (CNCs) were prepared as nanofillers to reinforce the P(Sar-co-GH)-OSA hydrogel.
35021414	2	2	theme	functional	322:331	arg1	polysaccharides					333:347	functional polysaccharides	322:347	functional polysaccharides	322:347	Herein, a composite hydrogel containing copolypept(o)ides and functional polysaccharides was constructed through dynamic acylhydrazone linkages.
35021414	9	3	theme	delivery	1526:1533	arg1	system					1535:1540	a customized responsive drug delivery system	1497:1540	a customized responsive drug delivery system	1497:1540	The NIH/3T3 cells encapsulated in the hydrogels maintained high viability and proliferation abilities, indicating that the nanocomposite hydrogels could be explored to fabricate a customized responsive drug delivery system or cell scaffolds for tissue engineering.
35021414	1	4	theme	biomedical	138:147	arg1	fields					149:154	biomedical fields	138:154	biomedical fields	138:154	The emerging tide of hydrogels in biomedical fields drives them to possess good biocompatibility, tunable mechanical properties, and fast gelation process.
35021414	2	5	theme	o	311:311	arg1	ides					313:316	copolypept(o)ides	300:316	copolypept(o)ides	300:316	Herein, a composite hydrogel containing copolypept(o)ides and functional polysaccharides was constructed through dynamic acylhydrazone linkages.
35021414	9	6	theme	cell	1545:1548	arg1	scaffolds					1550:1558	cell scaffolds	1545:1558	cell scaffolds	1545:1558	The NIH/3T3 cells encapsulated in the hydrogels maintained high viability and proliferation abilities, indicating that the nanocomposite hydrogels could be explored to fabricate a customized responsive drug delivery system or cell scaffolds for tissue engineering.
35021414	3	7	theme	γ-benzyl	538:545	arg1	BLG					554:556	BLG	554:556	BLG	554:556	First, a series of peptide-peptoid copolymers were synthesized by ring-opening polymerization of sarcosine (Sar) and l-glutamic acid γ-benzyl ester (BLG) N-carboxyanhydrides (NCAs).
35021414	3	7	theme	γ-benzyl	538:545	arg1	ester					547:551	l-glutamic acid γ-benzyl ester	522:551	l-glutamic acid γ-benzyl ester (BLG)	522:557	First, a series of peptide-peptoid copolymers were synthesized by ring-opening polymerization of sarcosine (Sar) and l-glutamic acid γ-benzyl ester (BLG) N-carboxyanhydrides (NCAs).
35021414	1	8	theme	gelation	242:249	arg1	process					251:257	fast gelation process	237:257	fast gelation process	237:257	The emerging tide of hydrogels in biomedical fields drives them to possess good biocompatibility, tunable mechanical properties, and fast gelation process.
35021414	5	9	theme	poly	736:739	arg1	precursor					817:825	an optimized precursor	804:825	an optimized precursor due to its excellent water solubility and gel-forming ability with aldehyde-modified sodium alginate (OSA)	804:932	The statistical copolymer of poly(sarcosine-co-glutamate-hydrazide) (P(Sar-co-GH)) was chosen as an optimized precursor due to its excellent water solubility and gel-forming ability with aldehyde-modified sodium alginate (OSA).
35021414	5	9	theme	poly	736:739	arg1	copolymer					723:731	The statistical copolymer	707:731	The statistical copolymer of poly(sarcosine-co-glutamate-hydrazide) (P(Sar-co-GH))	707:788	The statistical copolymer of poly(sarcosine-co-glutamate-hydrazide) (P(Sar-co-GH)) was chosen as an optimized precursor due to its excellent water solubility and gel-forming ability with aldehyde-modified sodium alginate (OSA).
35021414	5	10	with	ability	881:887	arg1	alginate					919:926	aldehyde-modified sodium alginate	894:926	aldehyde-modified sodium alginate (OSA)	894:932	The statistical copolymer of poly(sarcosine-co-glutamate-hydrazide) (P(Sar-co-GH)) was chosen as an optimized precursor due to its excellent water solubility and gel-forming ability with aldehyde-modified sodium alginate (OSA).
35021414	5	10	with	ability	881:887	arg1	OSA					929:931	OSA	929:931	OSA	929:931	The statistical copolymer of poly(sarcosine-co-glutamate-hydrazide) (P(Sar-co-GH)) was chosen as an optimized precursor due to its excellent water solubility and gel-forming ability with aldehyde-modified sodium alginate (OSA).
35021414	3	11	theme	ester	547:551	arg1	NCAs					580:583	NCAs	580:583	NCAs	580:583	First, a series of peptide-peptoid copolymers were synthesized by ring-opening polymerization of sarcosine (Sar) and l-glutamic acid γ-benzyl ester (BLG) N-carboxyanhydrides (NCAs).
35021414	3	11	theme	ester	547:551	arg1	N-carboxyanhydrides					559:577	sarcosine (Sar) and l-glutamic acid γ-benzyl ester (BLG) N-carboxyanhydrides	502:577	sarcosine (Sar) and l-glutamic acid γ-benzyl ester (BLG) N-carboxyanhydrides (NCAs)	502:584	First, a series of peptide-peptoid copolymers were synthesized by ring-opening polymerization of sarcosine (Sar) and l-glutamic acid γ-benzyl ester (BLG) N-carboxyanhydrides (NCAs).
35021414	7	12	theme	cross-linking	1198:1210	arg1	density					1212:1218	the cross-linking density	1194:1218	the cross-linking density	1194:1218	We demonstrated that the copolymer sequences and composition contents made a difference to the properties of the formed hydrogels by variation of the cross-linking density.
35021414	5	13	theme	excellent	838:846	arg1	solubility					854:863	its excellent water solubility	834:863	its excellent water solubility	834:863	The statistical copolymer of poly(sarcosine-co-glutamate-hydrazide) (P(Sar-co-GH)) was chosen as an optimized precursor due to its excellent water solubility and gel-forming ability with aldehyde-modified sodium alginate (OSA).
35021414	4	14	theme	aminolysis	686:695	arg1	reaction					697:704	ester-amide exchange aminolysis reaction	665:704	ester-amide exchange aminolysis reaction	665:704	The benzyl groups of BLG units were substituted with hydrazide groups through ester-amide exchange aminolysis reaction.
35021414	9	15	theme	responsive	1510:1519	arg1	system					1535:1540	a customized responsive drug delivery system	1497:1540	a customized responsive drug delivery system	1497:1540	The NIH/3T3 cells encapsulated in the hydrogels maintained high viability and proliferation abilities, indicating that the nanocomposite hydrogels could be explored to fabricate a customized responsive drug delivery system or cell scaffolds for tissue engineering.
35021414	4	16	theme	BLG	608:610	arg1	units					612:616	BLG units	608:616	BLG units	608:616	The benzyl groups of BLG units were substituted with hydrazide groups through ester-amide exchange aminolysis reaction.
35021414	3	17	theme	l-glutamic	522:531	arg1	BLG					554:556	BLG	554:556	BLG	554:556	First, a series of peptide-peptoid copolymers were synthesized by ring-opening polymerization of sarcosine (Sar) and l-glutamic acid γ-benzyl ester (BLG) N-carboxyanhydrides (NCAs).
35021414	3	17	theme	l-glutamic	522:531	arg1	ester					547:551	l-glutamic acid γ-benzyl ester	522:551	l-glutamic acid γ-benzyl ester (BLG)	522:557	First, a series of peptide-peptoid copolymers were synthesized by ring-opening polymerization of sarcosine (Sar) and l-glutamic acid γ-benzyl ester (BLG) N-carboxyanhydrides (NCAs).
35021414	2	18	theme	acylhydrazone	381:393	arg1	linkages					395:402	dynamic acylhydrazone linkages	373:402	dynamic acylhydrazone linkages	373:402	Herein, a composite hydrogel containing copolypept(o)ides and functional polysaccharides was constructed through dynamic acylhydrazone linkages.
35021414	7	19	theme	hydrogels	1168:1176	arg1	properties					1143:1152	the properties	1139:1152	the properties of the formed hydrogels	1139:1176	We demonstrated that the copolymer sequences and composition contents made a difference to the properties of the formed hydrogels by variation of the cross-linking density.
35021414	5	20	theme	aldehyde-modified	894:910	arg1	alginate					919:926	aldehyde-modified sodium alginate	894:926	aldehyde-modified sodium alginate (OSA)	894:932	The statistical copolymer of poly(sarcosine-co-glutamate-hydrazide) (P(Sar-co-GH)) was chosen as an optimized precursor due to its excellent water solubility and gel-forming ability with aldehyde-modified sodium alginate (OSA).
35021414	5	20	theme	aldehyde-modified	894:910	arg1	OSA					929:931	OSA	929:931	OSA	929:931	The statistical copolymer of poly(sarcosine-co-glutamate-hydrazide) (P(Sar-co-GH)) was chosen as an optimized precursor due to its excellent water solubility and gel-forming ability with aldehyde-modified sodium alginate (OSA).
35021414	7	21	theme	copolymer	1073:1081	arg1	sequences					1083:1091	copolymer sequences	1073:1091	copolymer sequences	1073:1091	We demonstrated that the copolymer sequences and composition contents made a difference to the properties of the formed hydrogels by variation of the cross-linking density.
35021414	3	22	theme	acid	533:536	arg1	BLG					554:556	BLG	554:556	BLG	554:556	First, a series of peptide-peptoid copolymers were synthesized by ring-opening polymerization of sarcosine (Sar) and l-glutamic acid γ-benzyl ester (BLG) N-carboxyanhydrides (NCAs).
35021414	3	22	theme	acid	533:536	arg1	ester					547:551	l-glutamic acid γ-benzyl ester	522:551	l-glutamic acid γ-benzyl ester (BLG)	522:557	First, a series of peptide-peptoid copolymers were synthesized by ring-opening polymerization of sarcosine (Sar) and l-glutamic acid γ-benzyl ester (BLG) N-carboxyanhydrides (NCAs).
35021414	2	23	theme	dynamic	373:379	arg1	linkages					395:402	dynamic acylhydrazone linkages	373:402	dynamic acylhydrazone linkages	373:402	Herein, a composite hydrogel containing copolypept(o)ides and functional polysaccharides was constructed through dynamic acylhydrazone linkages.
35021414	9	24	theme	NIH/3T3	1323:1329	arg1	cells					1331:1335	The NIH/3T3 cells	1319:1335	The NIH/3T3 cells encapsulated in the hydrogels	1319:1365	The NIH/3T3 cells encapsulated in the hydrogels maintained high viability and proliferation abilities, indicating that the nanocomposite hydrogels could be explored to fabricate a customized responsive drug delivery system or cell scaffolds for tissue engineering.
35021414	1	25	from	tide	117:120	arg1	fields					149:154	biomedical fields	138:154	biomedical fields	138:154	The emerging tide of hydrogels in biomedical fields drives them to possess good biocompatibility, tunable mechanical properties, and fast gelation process.
35021414	2	26	theme	copolypept	300:309	arg1	ides					313:316	copolypept(o)ides	300:316	copolypept(o)ides	300:316	Herein, a composite hydrogel containing copolypept(o)ides and functional polysaccharides was constructed through dynamic acylhydrazone linkages.
35021414	9	27	theme	drug	1521:1524	arg1	system					1535:1540	a customized responsive drug delivery system	1497:1540	a customized responsive drug delivery system	1497:1540	The NIH/3T3 cells encapsulated in the hydrogels maintained high viability and proliferation abilities, indicating that the nanocomposite hydrogels could be explored to fabricate a customized responsive drug delivery system or cell scaffolds for tissue engineering.
35021414	0	28	theme	In	0:1	arg1	Hydrogels					46:54	In Situ Forming and Reversibly Cross-Linkable Hydrogels	0:54	In Situ Forming and Reversibly Cross-Linkable Hydrogels	0:54	In Situ Forming and Reversibly Cross-Linkable Hydrogels Based on Copolypept(o)ides and Polysaccharides.
35021414	9	29	theme	high	1378:1381	arg1	abilities					1411:1419	high viability and proliferation abilities	1378:1419	high viability and proliferation abilities	1378:1419	The NIH/3T3 cells encapsulated in the hydrogels maintained high viability and proliferation abilities, indicating that the nanocomposite hydrogels could be explored to fabricate a customized responsive drug delivery system or cell scaffolds for tissue engineering.
35021414	3	30	theme	N-carboxyanhydrides	559:577	arg1	polymerization					484:497	ring-opening polymerization	471:497	ring-opening polymerization of sarcosine (Sar) and l-glutamic acid γ-benzyl ester (BLG) N-carboxyanhydrides (NCAs)	471:584	First, a series of peptide-peptoid copolymers were synthesized by ring-opening polymerization of sarcosine (Sar) and l-glutamic acid γ-benzyl ester (BLG) N-carboxyanhydrides (NCAs).
35021414	6	31	theme	-OSA	1033:1036	arg1	hydrogel					1038:1045	the P(Sar-co-GH)-OSA hydrogel	1017:1045	the P(Sar-co-GH)-OSA hydrogel	1017:1045	Moreover, cellulose nanocrystals (CNCs) were prepared as nanofillers to reinforce the P(Sar-co-GH)-OSA hydrogel.
35021414	8	32	theme	reversible	1298:1307	arg1	networks					1309:1316	reversible networks	1298:1316	reversible networks	1298:1316	The dynamic acylhydrazone bonds endowed hydrogels with pH responsiveness and reversible networks.
35021414	4	33	theme	units	612:616	arg1	groups					598:603	The benzyl groups	587:603	The benzyl groups of BLG units	587:616	The benzyl groups of BLG units were substituted with hydrazide groups through ester-amide exchange aminolysis reaction.
35021414	4	33	theme	units	612:616	arg1	units					612:616	BLG units	608:616	BLG units	608:616	The benzyl groups of BLG units were substituted with hydrazide groups through ester-amide exchange aminolysis reaction.
35021414	0	34	theme	Forming	8:14	arg1	Hydrogels					46:54	In Situ Forming and Reversibly Cross-Linkable Hydrogels	0:54	In Situ Forming and Reversibly Cross-Linkable Hydrogels	0:54	In Situ Forming and Reversibly Cross-Linkable Hydrogels Based on Copolypept(o)ides and Polysaccharides.
35021414	4	35	theme	benzyl	591:596	arg1	groups					598:603	The benzyl groups	587:603	The benzyl groups of BLG units	587:616	The benzyl groups of BLG units were substituted with hydrazide groups through ester-amide exchange aminolysis reaction.
35021414	4	35	theme	benzyl	591:596	arg1	units					612:616	BLG units	608:616	BLG units	608:616	The benzyl groups of BLG units were substituted with hydrazide groups through ester-amide exchange aminolysis reaction.
35021414	2	36	theme	composite	270:278	arg1	hydrogel					280:287	a composite hydrogel	268:287	a composite hydrogel containing copolypept(o)ides and functional polysaccharides	268:347	Herein, a composite hydrogel containing copolypept(o)ides and functional polysaccharides was constructed through dynamic acylhydrazone linkages.
35021414	9	37	theme	nanocomposite	1442:1454	arg1	hydrogels					1456:1464	the nanocomposite hydrogels	1438:1464	the nanocomposite hydrogels	1438:1464	The NIH/3T3 cells encapsulated in the hydrogels maintained high viability and proliferation abilities, indicating that the nanocomposite hydrogels could be explored to fabricate a customized responsive drug delivery system or cell scaffolds for tissue engineering.
35021414	1	38	theme	good	179:182	arg1	biocompatibility					184:199	good biocompatibility	179:199	good biocompatibility	179:199	The emerging tide of hydrogels in biomedical fields drives them to possess good biocompatibility, tunable mechanical properties, and fast gelation process.
35021414	0	39	theme	Cross-Linkable	31:44	arg1	Hydrogels					46:54	In Situ Forming and Reversibly Cross-Linkable Hydrogels	0:54	In Situ Forming and Reversibly Cross-Linkable Hydrogels	0:54	In Situ Forming and Reversibly Cross-Linkable Hydrogels Based on Copolypept(o)ides and Polysaccharides.
35021414	6	40	theme	cellulose	945:953	arg1	nanocrystals					955:966	cellulose nanocrystals	945:966	cellulose nanocrystals (CNCs)	945:973	Moreover, cellulose nanocrystals (CNCs) were prepared as nanofillers to reinforce the P(Sar-co-GH)-OSA hydrogel.
35021414	6	40	theme	cellulose	945:953	arg1	nanofillers					992:1002	nanofillers	992:1002	nanofillers	992:1002	Moreover, cellulose nanocrystals (CNCs) were prepared as nanofillers to reinforce the P(Sar-co-GH)-OSA hydrogel.
35021414	6	40	theme	cellulose	945:953	arg1	CNCs					969:972	CNCs	969:972	CNCs	969:972	Moreover, cellulose nanocrystals (CNCs) were prepared as nanofillers to reinforce the P(Sar-co-GH)-OSA hydrogel.
35021414	5	41	theme	statistical	711:721	arg1	precursor					817:825	an optimized precursor	804:825	an optimized precursor due to its excellent water solubility and gel-forming ability with aldehyde-modified sodium alginate (OSA)	804:932	The statistical copolymer of poly(sarcosine-co-glutamate-hydrazide) (P(Sar-co-GH)) was chosen as an optimized precursor due to its excellent water solubility and gel-forming ability with aldehyde-modified sodium alginate (OSA).
35021414	5	41	theme	statistical	711:721	arg1	copolymer					723:731	The statistical copolymer	707:731	The statistical copolymer of poly(sarcosine-co-glutamate-hydrazide) (P(Sar-co-GH))	707:788	The statistical copolymer of poly(sarcosine-co-glutamate-hydrazide) (P(Sar-co-GH)) was chosen as an optimized precursor due to its excellent water solubility and gel-forming ability with aldehyde-modified sodium alginate (OSA).
35021414	8	42	theme	dynamic	1225:1231	arg1	acylhydrazone					1233:1245	The dynamic acylhydrazone	1221:1245	The dynamic acylhydrazone	1221:1245	The dynamic acylhydrazone bonds endowed hydrogels with pH responsiveness and reversible networks.
35021414	3	43	theme	copolymers	440:449	arg1	series					414:419	a series	412:419	a series of peptide-peptoid copolymers	412:449	First, a series of peptide-peptoid copolymers were synthesized by ring-opening polymerization of sarcosine (Sar) and l-glutamic acid γ-benzyl ester (BLG) N-carboxyanhydrides (NCAs).
35021414	4	44	theme	hydrazide	640:648	arg1	groups					650:655	hydrazide groups	640:655	hydrazide groups	640:655	The benzyl groups of BLG units were substituted with hydrazide groups through ester-amide exchange aminolysis reaction.
35021414	5	45	theme	water	848:852	arg1	solubility					854:863	its excellent water solubility	834:863	its excellent water solubility	834:863	The statistical copolymer of poly(sarcosine-co-glutamate-hydrazide) (P(Sar-co-GH)) was chosen as an optimized precursor due to its excellent water solubility and gel-forming ability with aldehyde-modified sodium alginate (OSA).
35021414	1	46	theme	fast	237:240	arg1	process					251:257	fast gelation process	237:257	fast gelation process	237:257	The emerging tide of hydrogels in biomedical fields drives them to possess good biocompatibility, tunable mechanical properties, and fast gelation process.
35021414	9	47	theme	customized	1499:1508	arg1	system					1535:1540	a customized responsive drug delivery system	1497:1540	a customized responsive drug delivery system	1497:1540	The NIH/3T3 cells encapsulated in the hydrogels maintained high viability and proliferation abilities, indicating that the nanocomposite hydrogels could be explored to fabricate a customized responsive drug delivery system or cell scaffolds for tissue engineering.
35021414	6	48	theme	P	1021:1021	arg1	hydrogel					1038:1045	the P(Sar-co-GH)-OSA hydrogel	1017:1045	the P(Sar-co-GH)-OSA hydrogel	1017:1045	Moreover, cellulose nanocrystals (CNCs) were prepared as nanofillers to reinforce the P(Sar-co-GH)-OSA hydrogel.
35021414	5	49	theme	gel-forming	869:879	arg1	ability					881:887	gel-forming ability	869:887	gel-forming ability	869:887	The statistical copolymer of poly(sarcosine-co-glutamate-hydrazide) (P(Sar-co-GH)) was chosen as an optimized precursor due to its excellent water solubility and gel-forming ability with aldehyde-modified sodium alginate (OSA).
35021414	5	50	with	solubility	854:863	arg1	alginate					919:926	aldehyde-modified sodium alginate	894:926	aldehyde-modified sodium alginate (OSA)	894:932	The statistical copolymer of poly(sarcosine-co-glutamate-hydrazide) (P(Sar-co-GH)) was chosen as an optimized precursor due to its excellent water solubility and gel-forming ability with aldehyde-modified sodium alginate (OSA).
35021414	5	50	with	solubility	854:863	arg1	OSA					929:931	OSA	929:931	OSA	929:931	The statistical copolymer of poly(sarcosine-co-glutamate-hydrazide) (P(Sar-co-GH)) was chosen as an optimized precursor due to its excellent water solubility and gel-forming ability with aldehyde-modified sodium alginate (OSA).
35021414	5	51	theme	sodium	912:917	arg1	alginate					919:926	aldehyde-modified sodium alginate	894:926	aldehyde-modified sodium alginate (OSA)	894:932	The statistical copolymer of poly(sarcosine-co-glutamate-hydrazide) (P(Sar-co-GH)) was chosen as an optimized precursor due to its excellent water solubility and gel-forming ability with aldehyde-modified sodium alginate (OSA).
35021414	5	51	theme	sodium	912:917	arg1	OSA					929:931	OSA	929:931	OSA	929:931	The statistical copolymer of poly(sarcosine-co-glutamate-hydrazide) (P(Sar-co-GH)) was chosen as an optimized precursor due to its excellent water solubility and gel-forming ability with aldehyde-modified sodium alginate (OSA).
35021414	2	52	contain	containing	289:298	arg1	hydrogel					280:287	a composite hydrogel	268:287	a composite hydrogel containing copolypept(o)ides and functional polysaccharides	268:347	Herein, a composite hydrogel containing copolypept(o)ides and functional polysaccharides was constructed through dynamic acylhydrazone linkages.
35021414	2	52	contain	containing	289:298	arg2	polysaccharides					333:347	functional polysaccharides	322:347	functional polysaccharides	322:347	Herein, a composite hydrogel containing copolypept(o)ides and functional polysaccharides was constructed through dynamic acylhydrazone linkages.
35021414	2	52	contain	containing	289:298	arg2	ides					313:316	copolypept(o)ides	300:316	copolypept(o)ides	300:316	Herein, a composite hydrogel containing copolypept(o)ides and functional polysaccharides was constructed through dynamic acylhydrazone linkages.
35021414	3	53	theme	ring-opening	471:482	arg1	polymerization					484:497	ring-opening polymerization	471:497	ring-opening polymerization of sarcosine (Sar) and l-glutamic acid γ-benzyl ester (BLG) N-carboxyanhydrides (NCAs)	471:584	First, a series of peptide-peptoid copolymers were synthesized by ring-opening polymerization of sarcosine (Sar) and l-glutamic acid γ-benzyl ester (BLG) N-carboxyanhydrides (NCAs).
35021414	9	54	theme	tissue	1564:1569	arg1	engineering					1571:1581	tissue engineering	1564:1581	tissue engineering	1564:1581	The NIH/3T3 cells encapsulated in the hydrogels maintained high viability and proliferation abilities, indicating that the nanocomposite hydrogels could be explored to fabricate a customized responsive drug delivery system or cell scaffolds for tissue engineering.
35021414	3	55	theme	sarcosine	502:510	arg1	NCAs					580:583	NCAs	580:583	NCAs	580:583	First, a series of peptide-peptoid copolymers were synthesized by ring-opening polymerization of sarcosine (Sar) and l-glutamic acid γ-benzyl ester (BLG) N-carboxyanhydrides (NCAs).
35021414	3	55	theme	sarcosine	502:510	arg1	N-carboxyanhydrides					559:577	sarcosine (Sar) and l-glutamic acid γ-benzyl ester (BLG) N-carboxyanhydrides	502:577	sarcosine (Sar) and l-glutamic acid γ-benzyl ester (BLG) N-carboxyanhydrides (NCAs)	502:584	First, a series of peptide-peptoid copolymers were synthesized by ring-opening polymerization of sarcosine (Sar) and l-glutamic acid γ-benzyl ester (BLG) N-carboxyanhydrides (NCAs).
35021414	9	56	theme	viability	1383:1391	arg1	abilities					1411:1419	high viability and proliferation abilities	1378:1419	high viability and proliferation abilities	1378:1419	The NIH/3T3 cells encapsulated in the hydrogels maintained high viability and proliferation abilities, indicating that the nanocomposite hydrogels could be explored to fabricate a customized responsive drug delivery system or cell scaffolds for tissue engineering.
35021414	1	57	theme	emerging	108:115	arg1	tide					117:120	The emerging tide	104:120	The emerging tide of hydrogels in biomedical fields	104:154	The emerging tide of hydrogels in biomedical fields drives them to possess good biocompatibility, tunable mechanical properties, and fast gelation process.
35021414	1	58	theme	tunable	202:208	arg1	properties					221:230	tunable mechanical properties	202:230	tunable mechanical properties	202:230	The emerging tide of hydrogels in biomedical fields drives them to possess good biocompatibility, tunable mechanical properties, and fast gelation process.
35021414	5	59	dep	poly	736:739	arg1	Sar-co-GH					778:786	Sar-co-GH	778:786	Sar-co-GH	778:786	The statistical copolymer of poly(sarcosine-co-glutamate-hydrazide) (P(Sar-co-GH)) was chosen as an optimized precursor due to its excellent water solubility and gel-forming ability with aldehyde-modified sodium alginate (OSA).
35021414	5	59	dep	poly	736:739	arg1	P					776:776	P	776:776	P(Sar-co-GH)	776:787	The statistical copolymer of poly(sarcosine-co-glutamate-hydrazide) (P(Sar-co-GH)) was chosen as an optimized precursor due to its excellent water solubility and gel-forming ability with aldehyde-modified sodium alginate (OSA).
35021414	9	60	theme	proliferation	1397:1409	arg1	abilities					1411:1419	high viability and proliferation abilities	1378:1419	high viability and proliferation abilities	1378:1419	The NIH/3T3 cells encapsulated in the hydrogels maintained high viability and proliferation abilities, indicating that the nanocomposite hydrogels could be explored to fabricate a customized responsive drug delivery system or cell scaffolds for tissue engineering.
35021414	5	61	theme	optimized	807:815	arg1	precursor					817:825	an optimized precursor	804:825	an optimized precursor due to its excellent water solubility and gel-forming ability with aldehyde-modified sodium alginate (OSA)	804:932	The statistical copolymer of poly(sarcosine-co-glutamate-hydrazide) (P(Sar-co-GH)) was chosen as an optimized precursor due to its excellent water solubility and gel-forming ability with aldehyde-modified sodium alginate (OSA).
35021414	5	61	theme	optimized	807:815	arg1	copolymer					723:731	The statistical copolymer	707:731	The statistical copolymer of poly(sarcosine-co-glutamate-hydrazide) (P(Sar-co-GH))	707:788	The statistical copolymer of poly(sarcosine-co-glutamate-hydrazide) (P(Sar-co-GH)) was chosen as an optimized precursor due to its excellent water solubility and gel-forming ability with aldehyde-modified sodium alginate (OSA).
35021414	4	62	theme	ester-amide	665:675	arg1	reaction					697:704	ester-amide exchange aminolysis reaction	665:704	ester-amide exchange aminolysis reaction	665:704	The benzyl groups of BLG units were substituted with hydrazide groups through ester-amide exchange aminolysis reaction.
35021414	1	63	theme	mechanical	210:219	arg1	properties					221:230	tunable mechanical properties	202:230	tunable mechanical properties	202:230	The emerging tide of hydrogels in biomedical fields drives them to possess good biocompatibility, tunable mechanical properties, and fast gelation process.
35021414	0	64	theme	Copolypept	65:74	arg1	ides					78:81	Copolypept(o)ides	65:81	Copolypept(o)ides	65:81	In Situ Forming and Reversibly Cross-Linkable Hydrogels Based on Copolypept(o)ides and Polysaccharides.
35021414	1	65	contain	possess	171:177	arg1	them					163:166	them	163:166	them	163:166	The emerging tide of hydrogels in biomedical fields drives them to possess good biocompatibility, tunable mechanical properties, and fast gelation process.
35021414	1	65	contain	possess	171:177	arg2	process					251:257	fast gelation process	237:257	fast gelation process	237:257	The emerging tide of hydrogels in biomedical fields drives them to possess good biocompatibility, tunable mechanical properties, and fast gelation process.
35021414	1	65	contain	possess	171:177	arg2	biocompatibility					184:199	good biocompatibility	179:199	good biocompatibility	179:199	The emerging tide of hydrogels in biomedical fields drives them to possess good biocompatibility, tunable mechanical properties, and fast gelation process.
35021414	1	65	contain	possess	171:177	arg2	properties					221:230	tunable mechanical properties	202:230	tunable mechanical properties	202:230	The emerging tide of hydrogels in biomedical fields drives them to possess good biocompatibility, tunable mechanical properties, and fast gelation process.
35021414	8	66	theme	endowed	1253:1259	arg1	hydrogels					1261:1269	endowed hydrogels	1253:1269	endowed hydrogels	1253:1269	The dynamic acylhydrazone bonds endowed hydrogels with pH responsiveness and reversible networks.
35021414	4	67	theme	exchange	677:684	arg1	reaction					697:704	ester-amide exchange aminolysis reaction	665:704	ester-amide exchange aminolysis reaction	665:704	The benzyl groups of BLG units were substituted with hydrazide groups through ester-amide exchange aminolysis reaction.
35021414	3	68	theme	peptide-peptoid	424:438	arg1	copolymers					440:449	peptide-peptoid copolymers	424:449	peptide-peptoid copolymers	424:449	First, a series of peptide-peptoid copolymers were synthesized by ring-opening polymerization of sarcosine (Sar) and l-glutamic acid γ-benzyl ester (BLG) N-carboxyanhydrides (NCAs).
35021414	7	69	theme	density	1212:1218	arg1	variation					1181:1189	variation	1181:1189	variation of the cross-linking density	1181:1218	We demonstrated that the copolymer sequences and composition contents made a difference to the properties of the formed hydrogels by variation of the cross-linking density.
35021414	7	70	theme	formed	1161:1166	arg1	hydrogels					1168:1176	the formed hydrogels	1157:1176	the formed hydrogels	1157:1176	We demonstrated that the copolymer sequences and composition contents made a difference to the properties of the formed hydrogels by variation of the cross-linking density.
35021414	0	71	dep	In	0:1	arg1	Situ					3:6	Situ	3:6	Situ	3:6	In Situ Forming and Reversibly Cross-Linkable Hydrogels Based on Copolypept(o)ides and Polysaccharides.
35021414	1	72	theme	hydrogels	125:133	arg1	tide					117:120	The emerging tide	104:120	The emerging tide of hydrogels in biomedical fields	104:154	The emerging tide of hydrogels in biomedical fields drives them to possess good biocompatibility, tunable mechanical properties, and fast gelation process.
35021414	8	73	theme	pH	1276:1277	arg1	responsiveness					1279:1292	pH responsiveness	1276:1292	pH responsiveness	1276:1292	The dynamic acylhydrazone bonds endowed hydrogels with pH responsiveness and reversible networks.
29494830	2	0	from	nature	283:288	arg1	translucent					268:278	translucent	268:278	translucent	268:278	The PVA-CMT film was translucent in nature and smooth to touch.
29494830	2	0	from	nature	283:288	arg1	film					259:262	The PVA-CMT film	247:262	The PVA-CMT film	247:262	The PVA-CMT film was translucent in nature and smooth to touch.
29494830	1	1	theme	films	240:244	arg1	preparation					185:195	the preparation	181:195	the preparation	181:195	The current study deals with the preparation and the characterization of the PVA-CMT-GO films.
29494830	1	1	theme	films	240:244	arg1	characterization					205:220	the characterization	201:220	the characterization	201:220	The current study deals with the preparation and the characterization of the PVA-CMT-GO films.
29494830	0	2	theme	poly	72:75	arg1	properties					58:67	the properties	54:67	the properties of poly (vinyl alcohol) and carboxymethyl tamarind gum based phase-separated film	54:149	Reinforcing effect of graphene oxide reinforcement on the properties of poly (vinyl alcohol) and carboxymethyl tamarind gum based phase-separated film.
29494830	0	3	from	effect	12:17	arg1	properties					58:67	the properties	54:67	the properties of poly (vinyl alcohol) and carboxymethyl tamarind gum based phase-separated film	54:149	Reinforcing effect of graphene oxide reinforcement on the properties of poly (vinyl alcohol) and carboxymethyl tamarind gum based phase-separated film.
29494830	6	4	from	increase	699:706	arg1	content					718:724	the GO content	711:724	the GO content	711:724	The drug release study showed a decrease in the amount of the drug release with the increase in the GO content.
29494830	7	5	theme	inherent	824:831	arg1	property					847:854	the inherent antimicrobial property	820:854	the inherent antimicrobial property of GO	820:860	The PVA-CMT-GO films (without drug) showed certain degree of antimicrobial activity owing to the inherent antimicrobial property of GO.
29494830	2	6	from	smooth	294:299	arg1	translucent					268:278	translucent	268:278	translucent	268:278	The PVA-CMT film was translucent in nature and smooth to touch.
29494830	2	6	from	smooth	294:299	arg1	film					259:262	The PVA-CMT film	247:262	The PVA-CMT film	247:262	The PVA-CMT film was translucent in nature and smooth to touch.
29494830	0	7	theme	vinyl	78:82	arg1	poly					72:75	poly	72:75	poly (vinyl alcohol)	72:91	Reinforcing effect of graphene oxide reinforcement on the properties of poly (vinyl alcohol) and carboxymethyl tamarind gum based phase-separated film.
29494830	0	7	theme	vinyl	78:82	arg1	alcohol					84:90	vinyl alcohol	78:90	vinyl alcohol	78:90	Reinforcing effect of graphene oxide reinforcement on the properties of poly (vinyl alcohol) and carboxymethyl tamarind gum based phase-separated film.
29494830	4	8	theme	crystallite	457:467	arg1	size					469:472	the average crystallite size	445:472	the average crystallite size	445:472	XRD studies suggested that the incorporation of GO increased the average crystallite size.
29494830	8	9	theme	loaded	872:877	arg1	films					879:883	The drug loaded films	863:883	The drug loaded films	863:883	The drug loaded films also showed good antimicrobial property.
29494830	6	10	theme	release	682:688	arg1	amount					663:668	the amount	659:668	the amount of the drug release	659:688	The drug release study showed a decrease in the amount of the drug release with the increase in the GO content.
29494830	6	10	theme	release	682:688	arg1	release					682:688	the drug release	673:688	the drug release	673:688	The drug release study showed a decrease in the amount of the drug release with the increase in the GO content.
29494830	4	11	theme	average	449:455	arg1	size					469:472	the average crystallite size	445:472	the average crystallite size	445:472	XRD studies suggested that the incorporation of GO increased the average crystallite size.
29494830	4	12	theme	XRD	384:386	arg1	studies					388:394	XRD studies	384:394	XRD studies	384:394	XRD studies suggested that the incorporation of GO increased the average crystallite size.
29494830	5	13	theme	mechanical	479:488	arg1	properties					490:499	The mechanical properties	475:499	The mechanical properties of the films as determined by stress relaxation studies	475:555	The mechanical properties of the films as determined by stress relaxation studies suggested that all the films were viscoelastic in nature.
29494830	1	14	theme	current	156:162	arg1	study					164:168	The current study	152:168	The current study	152:168	The current study deals with the preparation and the characterization of the PVA-CMT-GO films.
29494830	3	15	theme	GO	327:328	arg1	addition					315:322	The addition	311:322	The addition of GO	311:328	The addition of GO resulted in the formation of agglomerated structures.
29494830	7	16	theme	activity	802:809	arg1	degree					778:783	certain degree	770:783	certain degree of antimicrobial activity owing to the inherent antimicrobial property of GO	770:860	The PVA-CMT-GO films (without drug) showed certain degree of antimicrobial activity owing to the inherent antimicrobial property of GO.
29494830	9	17	theme	human	1001:1005	arg1	keratinocytes					1012:1024	the human skin keratinocytes	997:1024	the human skin keratinocytes	997:1024	It was found that the prepared films altered the cell proliferation of the human skin keratinocytes in a composition-dependent manner.
29494830	3	18	theme	agglomerated	359:370	arg1	structures					372:381	agglomerated structures	359:381	agglomerated structures	359:381	The addition of GO resulted in the formation of agglomerated structures.
29494830	6	19	from	decrease	647:654	arg1	amount					663:668	the amount	659:668	the amount of the drug release	659:688	The drug release study showed a decrease in the amount of the drug release with the increase in the GO content.
29494830	6	19	from	decrease	647:654	arg1	release					682:688	the drug release	673:688	the drug release	673:688	The drug release study showed a decrease in the amount of the drug release with the increase in the GO content.
29494830	0	20	theme	graphene	22:29	arg1	reinforcement					37:49	graphene oxide reinforcement	22:49	graphene oxide reinforcement	22:49	Reinforcing effect of graphene oxide reinforcement on the properties of poly (vinyl alcohol) and carboxymethyl tamarind gum based phase-separated film.
29494830	0	21	theme	tamarind	111:118	arg1	film					146:149	carboxymethyl tamarind gum based phase-separated film	97:149	carboxymethyl tamarind gum based phase-separated film	97:149	Reinforcing effect of graphene oxide reinforcement on the properties of poly (vinyl alcohol) and carboxymethyl tamarind gum based phase-separated film.
29494830	7	22	theme	antimicrobial	788:800	arg1	activity					802:809	antimicrobial activity	788:809	antimicrobial activity owing to the inherent antimicrobial property of GO	788:860	The PVA-CMT-GO films (without drug) showed certain degree of antimicrobial activity owing to the inherent antimicrobial property of GO.
29494830	8	23	theme	good	897:900	arg1	property					916:923	good antimicrobial property	897:923	good antimicrobial property	897:923	The drug loaded films also showed good antimicrobial property.
29494830	5	24	from	nature	607:612	arg1	viscoelastic					591:602	viscoelastic	591:602	viscoelastic	591:602	The mechanical properties of the films as determined by stress relaxation studies suggested that all the films were viscoelastic in nature.
29494830	5	24	from	nature	607:612	arg1	films					580:584	all the films	572:584	all the films	572:584	The mechanical properties of the films as determined by stress relaxation studies suggested that all the films were viscoelastic in nature.
29494830	0	25	theme	carboxymethyl	97:109	arg1	film					146:149	carboxymethyl tamarind gum based phase-separated film	97:149	carboxymethyl tamarind gum based phase-separated film	97:149	Reinforcing effect of graphene oxide reinforcement on the properties of poly (vinyl alcohol) and carboxymethyl tamarind gum based phase-separated film.
29494830	4	26	theme	GO	432:433	arg1	incorporation					415:427	the incorporation	411:427	the incorporation of GO	411:433	XRD studies suggested that the incorporation of GO increased the average crystallite size.
29494830	7	27	theme	certain	770:776	arg1	degree					778:783	certain degree	770:783	certain degree of antimicrobial activity owing to the inherent antimicrobial property of GO	770:860	The PVA-CMT-GO films (without drug) showed certain degree of antimicrobial activity owing to the inherent antimicrobial property of GO.
29494830	0	28	theme	reinforcement	37:49	arg1	effect					12:17	effect	12:17	effect of graphene oxide reinforcement on the properties of poly (vinyl alcohol) and carboxymethyl tamarind gum based phase-separated film	12:149	Reinforcing effect of graphene oxide reinforcement on the properties of poly (vinyl alcohol) and carboxymethyl tamarind gum based phase-separated film.
29494830	6	29	theme	drug	677:680	arg1	release					682:688	the drug release	673:688	the drug release	673:688	The drug release study showed a decrease in the amount of the drug release with the increase in the GO content.
29494830	0	30	theme	based	124:128	arg1	film					146:149	carboxymethyl tamarind gum based phase-separated film	97:149	carboxymethyl tamarind gum based phase-separated film	97:149	Reinforcing effect of graphene oxide reinforcement on the properties of poly (vinyl alcohol) and carboxymethyl tamarind gum based phase-separated film.
29494830	9	31	theme	skin	1007:1010	arg1	keratinocytes					1012:1024	the human skin keratinocytes	997:1024	the human skin keratinocytes	997:1024	It was found that the prepared films altered the cell proliferation of the human skin keratinocytes in a composition-dependent manner.
29494830	5	32	dep	films	508:512	arg1	determined					517:526	determined	517:526	determined by stress relaxation studies	517:555	The mechanical properties of the films as determined by stress relaxation studies suggested that all the films were viscoelastic in nature.
29494830	0	33	theme	oxide	31:35	arg1	reinforcement					37:49	graphene oxide reinforcement	22:49	graphene oxide reinforcement	22:49	Reinforcing effect of graphene oxide reinforcement on the properties of poly (vinyl alcohol) and carboxymethyl tamarind gum based phase-separated film.
29494830	0	34	theme	gum	120:122	arg1	film					146:149	carboxymethyl tamarind gum based phase-separated film	97:149	carboxymethyl tamarind gum based phase-separated film	97:149	Reinforcing effect of graphene oxide reinforcement on the properties of poly (vinyl alcohol) and carboxymethyl tamarind gum based phase-separated film.
29494830	3	35	theme	structures	372:381	arg1	formation					346:354	the formation	342:354	the formation of agglomerated structures	342:381	The addition of GO resulted in the formation of agglomerated structures.
29494830	0	36	theme	film	146:149	arg1	properties					58:67	the properties	54:67	the properties of poly (vinyl alcohol) and carboxymethyl tamarind gum based phase-separated film	54:149	Reinforcing effect of graphene oxide reinforcement on the properties of poly (vinyl alcohol) and carboxymethyl tamarind gum based phase-separated film.
29494830	5	37	theme	stress	531:536	arg1	studies					549:555	stress relaxation studies	531:555	stress relaxation studies	531:555	The mechanical properties of the films as determined by stress relaxation studies suggested that all the films were viscoelastic in nature.
29494830	0	38	theme	phase-separated	130:144	arg1	film					146:149	carboxymethyl tamarind gum based phase-separated film	97:149	carboxymethyl tamarind gum based phase-separated film	97:149	Reinforcing effect of graphene oxide reinforcement on the properties of poly (vinyl alcohol) and carboxymethyl tamarind gum based phase-separated film.
29494830	9	39	theme	prepared	948:955	arg1	films					957:961	the prepared films	944:961	the prepared films	944:961	It was found that the prepared films altered the cell proliferation of the human skin keratinocytes in a composition-dependent manner.
29494830	5	40	theme	relaxation	538:547	arg1	studies					549:555	stress relaxation studies	531:555	stress relaxation studies	531:555	The mechanical properties of the films as determined by stress relaxation studies suggested that all the films were viscoelastic in nature.
29494830	7	41	theme	PVA-CMT-GO	731:740	arg1	films					742:746	The PVA-CMT-GO films	727:746	The PVA-CMT-GO films (without drug)	727:761	The PVA-CMT-GO films (without drug) showed certain degree of antimicrobial activity owing to the inherent antimicrobial property of GO.
29494830	9	42	theme	keratinocytes	1012:1024	arg1	proliferation					980:992	the cell proliferation	971:992	the cell proliferation of the human skin keratinocytes	971:1024	It was found that the prepared films altered the cell proliferation of the human skin keratinocytes in a composition-dependent manner.
29494830	2	43	theme	PVA-CMT	251:257	arg1	translucent					268:278	translucent	268:278	translucent	268:278	The PVA-CMT film was translucent in nature and smooth to touch.
29494830	2	43	theme	PVA-CMT	251:257	arg1	film					259:262	The PVA-CMT film	247:262	The PVA-CMT film	247:262	The PVA-CMT film was translucent in nature and smooth to touch.
29494830	9	44	theme	cell	975:978	arg1	proliferation					980:992	the cell proliferation	971:992	the cell proliferation of the human skin keratinocytes	971:1024	It was found that the prepared films altered the cell proliferation of the human skin keratinocytes in a composition-dependent manner.
29494830	8	45	theme	antimicrobial	902:914	arg1	property					916:923	good antimicrobial property	897:923	good antimicrobial property	897:923	The drug loaded films also showed good antimicrobial property.
29494830	7	46	theme	antimicrobial	833:845	arg1	property					847:854	the inherent antimicrobial property	820:854	the inherent antimicrobial property of GO	820:860	The PVA-CMT-GO films (without drug) showed certain degree of antimicrobial activity owing to the inherent antimicrobial property of GO.
29494830	7	47	theme	GO	859:860	arg1	property					847:854	the inherent antimicrobial property	820:854	the inherent antimicrobial property of GO	820:860	The PVA-CMT-GO films (without drug) showed certain degree of antimicrobial activity owing to the inherent antimicrobial property of GO.
29494830	9	48	theme	composition-dependent	1031:1051	arg1	manner					1053:1058	a composition-dependent manner	1029:1058	a composition-dependent manner	1029:1058	It was found that the prepared films altered the cell proliferation of the human skin keratinocytes in a composition-dependent manner.
29494830	6	49	dep	content	718:724	arg1	GO					715:716	GO	715:716	GO	715:716	The drug release study showed a decrease in the amount of the drug release with the increase in the GO content.
29494830	8	50	dep	loaded	872:877	arg1	drug					867:870	drug	867:870	drug	867:870	The drug loaded films also showed good antimicrobial property.
29494830	5	51	theme	films	508:512	arg1	properties					490:499	The mechanical properties	475:499	The mechanical properties of the films as determined by stress relaxation studies	475:555	The mechanical properties of the films as determined by stress relaxation studies suggested that all the films were viscoelastic in nature.
29494830	5	52	from	viscoelastic	591:602	arg1	nature					607:612	nature	607:612	nature	607:612	The mechanical properties of the films as determined by stress relaxation studies suggested that all the films were viscoelastic in nature.
29494830	2	53	from	translucent	268:278	arg1	smooth					294:299	smooth	294:299	smooth	294:299	The PVA-CMT film was translucent in nature and smooth to touch.
29494830	2	53	from	translucent	268:278	arg1	nature					283:288	nature	283:288	nature	283:288	The PVA-CMT film was translucent in nature and smooth to touch.
29494830	6	54	theme	release	624:630	arg1	study					632:636	The drug release study	615:636	The drug release study	615:636	The drug release study showed a decrease in the amount of the drug release with the increase in the GO content.
29494830	6	55	theme	drug	619:622	arg1	study					632:636	The drug release study	615:636	The drug release study	615:636	The drug release study showed a decrease in the amount of the drug release with the increase in the GO content.
29494830	1	56	theme	PVA-CMT-GO	229:238	arg1	films					240:244	the PVA-CMT-GO films	225:244	the PVA-CMT-GO films	225:244	The current study deals with the preparation and the characterization of the PVA-CMT-GO films.
31846802	11	0	theme	drug	2139:2142	arg1	resistance					2144:2153	drug resistance	2139:2153	drug resistance	2139:2153	In addition, this work will contribute toward the development of urgently needed tumor nanotherapy that is able to overcome drug resistance while also offering an unmatched range of effective therapeutic nanocarriers.
31846802	2	1	from	membrane	567:574	arg1	located					543:549	located	543:549	located	543:549	Drug resistance has been associated with the high expression of the multi-drug resistance gene 1 (MDR1), which can encode an efflux transporter known as P-glycoprotein (P-gp) that is located in the cellular membrane.
31846802	6	2	theme	redox-cleavable	1281:1295	arg1	nanogels					1311:1318	redox-cleavable dextran-based nanogels	1281:1318	redox-cleavable dextran-based nanogels	1281:1318	This nanocarrier was fabricated via the frame-guided self-assembly of lipid amphiphiles on the surfaces of redox-cleavable dextran-based nanogels.
31846802	8	3	theme	cancer	1658:1663	arg1	cells					1665:1669	highly PTX-resistant cancer cells	1637:1669	highly PTX-resistant cancer cells	1637:1669	MDR1-siRNA can knock down MDR1, promoting the accumulation of PTX in cells, and thus is expected to achieve an efficient inhibitory effect against highly PTX-resistant cancer cells.
31846802	1	4	theme	significant	302:312	arg1	challenge					314:322	a significant challenge	300:322	a significant challenge	300:322	While drug resistance has been recognized as the main cause of unsuccessful chemotherapy, the efficient inhibition of highly drug-resistant tumors still remains a significant challenge, especially for in vivo treatments.
31846802	7	5	theme	hydrophobic	1436:1446	arg1	core					1457:1460	the hydrophobic internal core	1432:1460	the hydrophobic internal core of the hybrid nanocarriers	1432:1487	The anionic MDR1-siRNA and the hydrophobic drug PTX were respectively loaded into the cationic lipid shell and the hydrophobic internal core of the hybrid nanocarriers.
31846802	10	6	theme	drug-resistant	1966:1979	arg1	tumors					1981:1986	highly drug-resistant tumors	1959:1986	highly drug-resistant tumors	1959:1986	Thus, the construction of gene/chemo co-delivered lipid/dextran nanocarriers provides a new strategy to inhibit highly drug-resistant tumors both in vitro and in vivo.
31846802	0	7	theme	Gene/paclitaxel	0:14	arg1	nanocarriers					30:41	Gene/paclitaxel co-delivering nanocarriers	0:41	Gene/paclitaxel co-delivering nanocarriers	0:41	Gene/paclitaxel co-delivering nanocarriers prepared by framework-induced self-assembly for the inhibition of highly drug-resistant tumors.
31846802	7	8	theme	hybrid	1469:1474	arg1	nanocarriers					1476:1487	the hybrid nanocarriers	1465:1487	the hybrid nanocarriers	1465:1487	The anionic MDR1-siRNA and the hydrophobic drug PTX were respectively loaded into the cationic lipid shell and the hydrophobic internal core of the hybrid nanocarriers.
31846802	9	9	theme	in	1690:1691	arg1	studies					1698:1704	Both in vitro and in vivo studies	1672:1704	Both in vitro and in vivo studies	1672:1704	Both in vitro and in vivo studies revealed that this dual-delivery system significantly enhanced the therapeutic effect in comparison with that provided by a PTX-only system.
31846802	6	10	theme	lipid	1244:1248	arg1	amphiphiles					1250:1260	lipid amphiphiles	1244:1260	lipid amphiphiles	1244:1260	This nanocarrier was fabricated via the frame-guided self-assembly of lipid amphiphiles on the surfaces of redox-cleavable dextran-based nanogels.
31846802	1	11	theme	chemotherapy	215:226	arg1	cause					193:197	the main cause	184:197	the main cause of unsuccessful chemotherapy	184:226	While drug resistance has been recognized as the main cause of unsuccessful chemotherapy, the efficient inhibition of highly drug-resistant tumors still remains a significant challenge, especially for in vivo treatments.
31846802	1	11	theme	chemotherapy	215:226	arg1	resistance					150:159	drug resistance	145:159	drug resistance	145:159	While drug resistance has been recognized as the main cause of unsuccessful chemotherapy, the efficient inhibition of highly drug-resistant tumors still remains a significant challenge, especially for in vivo treatments.
31846802	4	12	theme	gene/drug	903:911	arg1	nanocarrier					925:935	an efficient gene/drug co-delivery nanocarrier	890:935	an efficient gene/drug co-delivery nanocarrier	890:935	Herein, highly paclitaxel (PTX)-resistant ovarian (OV) cancer with a drug resistance index reaching up to ~ 60 was chosen to evaluate the performance of an efficient gene/drug co-delivery nanocarrier.
31846802	7	13	theme	lipid	1416:1420	arg1	shell					1422:1426	the cationic lipid shell	1403:1426	the cationic lipid shell	1403:1426	The anionic MDR1-siRNA and the hydrophobic drug PTX were respectively loaded into the cationic lipid shell and the hydrophobic internal core of the hybrid nanocarriers.
31846802	13	14	theme	co-loaded	2552:2560	arg1	nanocarriers					2594:2605	The MDR1-siRNA/PTX co-loaded biomimetic lipid/dextran hybrid nanocarriers	2533:2605	The MDR1-siRNA/PTX co-loaded biomimetic lipid/dextran hybrid nanocarriers	2533:2605	The MDR1-siRNA/PTX co-loaded biomimetic lipid/dextran hybrid nanocarriers demonstrate good capability in overcoming the PTX-resistance in highly chemo-resistant human ovarian (OV) cancer cells both in vitro and in vivo.
31846802	4	15	theme	nanocarrier	925:935	arg1	performance					875:885	the performance	871:885	the performance of an efficient gene/drug co-delivery nanocarrier	871:935	Herein, highly paclitaxel (PTX)-resistant ovarian (OV) cancer with a drug resistance index reaching up to ~ 60 was chosen to evaluate the performance of an efficient gene/drug co-delivery nanocarrier.
31846802	12	16	theme	frame-guided	2366:2377	arg1	self-assembly					2379:2391	the frame-guided self-assembly	2362:2391	the frame-guided self-assembly of lipid amphiphiles	2362:2412	STATEMENT OF SIGNIFICANCE: The biomimetic lipid/dextran hybrid nanocarrier with a diameter of ~ 100 nm, which was fabricated via the frame-guided self-assembly of lipid amphiphiles onto the surface of redox-cleavable dextran-based nanogels, provides a model carrier to co-deliver MDR1-siRNA and PTX.
31846802	13	17	theme	lipid/dextran	2573:2585	arg1	nanocarriers					2594:2605	The MDR1-siRNA/PTX co-loaded biomimetic lipid/dextran hybrid nanocarriers	2533:2605	The MDR1-siRNA/PTX co-loaded biomimetic lipid/dextran hybrid nanocarriers	2533:2605	The MDR1-siRNA/PTX co-loaded biomimetic lipid/dextran hybrid nanocarriers demonstrate good capability in overcoming the PTX-resistance in highly chemo-resistant human ovarian (OV) cancer cells both in vitro and in vivo.
31846802	11	18	theme	unmatched	2178:2186	arg1	range					2188:2192	an unmatched range	2175:2192	an unmatched range of effective therapeutic nanocarriers	2175:2230	In addition, this work will contribute toward the development of urgently needed tumor nanotherapy that is able to overcome drug resistance while also offering an unmatched range of effective therapeutic nanocarriers.
31846802	9	19	theme	dual-delivery	1725:1737	arg1	system					1739:1744	this dual-delivery system	1720:1744	this dual-delivery system	1720:1744	Both in vitro and in vivo studies revealed that this dual-delivery system significantly enhanced the therapeutic effect in comparison with that provided by a PTX-only system.
31846802	6	20	theme	frame-guided	1214:1225	arg1	self-assembly					1227:1239	the frame-guided self-assembly	1210:1239	the frame-guided self-assembly of lipid amphiphiles on the surfaces of redox-cleavable dextran-based nanogels	1210:1318	This nanocarrier was fabricated via the frame-guided self-assembly of lipid amphiphiles on the surfaces of redox-cleavable dextran-based nanogels.
31846802	5	21	theme	lipid/dextran	1028:1040	arg1	nanocarrier					1049:1059	a biomimetic lipid/dextran hybrid nanocarrier	1015:1059	a biomimetic lipid/dextran hybrid nanocarrier with a diameter of ~ 100 nm	1015:1087	Inspired by the self-assembly that occurs in cells and exosomes, we designed a biomimetic lipid/dextran hybrid nanocarrier with a diameter of ~ 100 nm to enhance the endocytosis and the efficiency of drug/gene release within the cells.
31846802	11	22	theme	nanotherapy	2102:2112	arg1	development					2065:2075	the development	2061:2075	the development of urgently needed tumor nanotherapy that is able to overcome drug resistance while also offering an unmatched range of effective therapeutic nanocarriers	2061:2230	In addition, this work will contribute toward the development of urgently needed tumor nanotherapy that is able to overcome drug resistance while also offering an unmatched range of effective therapeutic nanocarriers.
31846802	6	23	theme	nanogels	1311:1318	arg1	surfaces					1269:1276	the surfaces	1265:1276	the surfaces of redox-cleavable dextran-based nanogels	1265:1318	This nanocarrier was fabricated via the frame-guided self-assembly of lipid amphiphiles on the surfaces of redox-cleavable dextran-based nanogels.
31846802	7	24	theme	drug	1364:1367	arg1	PTX					1369:1371	the hydrophobic drug PTX	1348:1371	the hydrophobic drug PTX	1348:1371	The anionic MDR1-siRNA and the hydrophobic drug PTX were respectively loaded into the cationic lipid shell and the hydrophobic internal core of the hybrid nanocarriers.
31846802	6	25	from	self-assembly	1227:1239	arg1	surfaces					1269:1276	the surfaces	1265:1276	the surfaces of redox-cleavable dextran-based nanogels	1265:1318	This nanocarrier was fabricated via the frame-guided self-assembly of lipid amphiphiles on the surfaces of redox-cleavable dextran-based nanogels.
31846802	3	26	theme	MDR1-inhibited	613:626	arg1	genes					628:632	MDR1-inhibited genes	613:632	MDR1-inhibited genes	613:632	Therefore, the combined delivery of MDR1-inhibited genes and chemotherapeutic drugs is anticipated to enable the effective inhibition of drug-resistant tumors.
31846802	12	27	theme	~	2327:2327	arg1	100 nm					2329:2334	~ 100 nm	2327:2334	~ 100 nm	2327:2334	STATEMENT OF SIGNIFICANCE: The biomimetic lipid/dextran hybrid nanocarrier with a diameter of ~ 100 nm, which was fabricated via the frame-guided self-assembly of lipid amphiphiles onto the surface of redox-cleavable dextran-based nanogels, provides a model carrier to co-deliver MDR1-siRNA and PTX.
31846802	1	28	theme	main	188:191	arg1	cause					193:197	the main cause	184:197	the main cause of unsuccessful chemotherapy	184:226	While drug resistance has been recognized as the main cause of unsuccessful chemotherapy, the efficient inhibition of highly drug-resistant tumors still remains a significant challenge, especially for in vivo treatments.
31846802	1	28	theme	main	188:191	arg1	resistance					150:159	drug resistance	145:159	drug resistance	145:159	While drug resistance has been recognized as the main cause of unsuccessful chemotherapy, the efficient inhibition of highly drug-resistant tumors still remains a significant challenge, especially for in vivo treatments.
31846802	4	29	theme	ovarian	779:785	arg1	cancer					792:797	highly paclitaxel (PTX)-resistant ovarian (OV) cancer	745:797	highly paclitaxel (PTX)-resistant ovarian (OV) cancer with a drug resistance index reaching up to ~ 60	745:846	Herein, highly paclitaxel (PTX)-resistant ovarian (OV) cancer with a drug resistance index reaching up to ~ 60 was chosen to evaluate the performance of an efficient gene/drug co-delivery nanocarrier.
31846802	4	29	theme	ovarian	779:785	arg1	OV					788:789	OV	788:789	OV	788:789	Herein, highly paclitaxel (PTX)-resistant ovarian (OV) cancer with a drug resistance index reaching up to ~ 60 was chosen to evaluate the performance of an efficient gene/drug co-delivery nanocarrier.
31846802	4	30	with	cancer	792:797	arg1	index					822:826	a drug resistance index	804:826	a drug resistance index reaching up to ~ 60	804:846	Herein, highly paclitaxel (PTX)-resistant ovarian (OV) cancer with a drug resistance index reaching up to ~ 60 was chosen to evaluate the performance of an efficient gene/drug co-delivery nanocarrier.
31846802	0	31	theme	drug-resistant	116:129	arg1	tumors					131:136	highly drug-resistant tumors	109:136	highly drug-resistant tumors	109:136	Gene/paclitaxel co-delivering nanocarriers prepared by framework-induced self-assembly for the inhibition of highly drug-resistant tumors.
31846802	5	32	theme	100 nm	1082:1087	arg1	diameter					1068:1075	a diameter	1066:1075	a diameter of ~ 100 nm	1066:1087	Inspired by the self-assembly that occurs in cells and exosomes, we designed a biomimetic lipid/dextran hybrid nanocarrier with a diameter of ~ 100 nm to enhance the endocytosis and the efficiency of drug/gene release within the cells.
31846802	2	33	theme	multi-drug	428:437	arg1	gene					450:453	multi-drug resistance gene 1	428:455	the multi-drug resistance gene 1 (MDR1)	424:462	Drug resistance has been associated with the high expression of the multi-drug resistance gene 1 (MDR1), which can encode an efflux transporter known as P-glycoprotein (P-gp) that is located in the cellular membrane.
31846802	2	33	theme	multi-drug	428:437	arg1	MDR1					458:461	MDR1	458:461	MDR1	458:461	Drug resistance has been associated with the high expression of the multi-drug resistance gene 1 (MDR1), which can encode an efflux transporter known as P-glycoprotein (P-gp) that is located in the cellular membrane.
31846802	2	33	theme	multi-drug	428:437	arg1	transporter					492:502	an efflux transporter	482:502	an efflux transporter known as P-glycoprotein (P-gp) that is located in the cellular membrane	482:574	Drug resistance has been associated with the high expression of the multi-drug resistance gene 1 (MDR1), which can encode an efflux transporter known as P-glycoprotein (P-gp) that is located in the cellular membrane.
31846802	0	34	theme	framework-induced	55:71	arg1	self-assembly					73:85	framework-induced self-assembly	55:85	framework-induced self-assembly for the inhibition of highly drug-resistant tumors	55:136	Gene/paclitaxel co-delivering nanocarriers prepared by framework-induced self-assembly for the inhibition of highly drug-resistant tumors.
31846802	7	35	theme	hydrophobic	1352:1362	arg1	PTX					1369:1371	the hydrophobic drug PTX	1348:1371	the hydrophobic drug PTX	1348:1371	The anionic MDR1-siRNA and the hydrophobic drug PTX were respectively loaded into the cationic lipid shell and the hydrophobic internal core of the hybrid nanocarriers.
31846802	8	36	theme	PTX	1552:1554	arg1	accumulation					1536:1547	the accumulation	1532:1547	the accumulation of PTX in cells	1532:1563	MDR1-siRNA can knock down MDR1, promoting the accumulation of PTX in cells, and thus is expected to achieve an efficient inhibitory effect against highly PTX-resistant cancer cells.
31846802	12	37	theme	SIGNIFICANCE	2246:2257	arg1	STATEMENT					2233:2241	STATEMENT	2233:2241	STATEMENT OF SIGNIFICANCE: The biomimetic lipid/dextran hybrid nanocarrier with a diameter of ~ 100 nm, which was fabricated via the frame-guided self-assembly of lipid amphiphiles onto the surface of redox-cleavable dextran-based nanogels, provides a model carrier to co-deliver MDR1-siRNA and PTX.	2233:2531	STATEMENT OF SIGNIFICANCE: The biomimetic lipid/dextran hybrid nanocarrier with a diameter of ~ 100 nm, which was fabricated via the frame-guided self-assembly of lipid amphiphiles onto the surface of redox-cleavable dextran-based nanogels, provides a model carrier to co-deliver MDR1-siRNA and PTX.
31846802	4	38	dep	~	843:843	arg1	up					837:838	up	837:838	up	837:838	Herein, highly paclitaxel (PTX)-resistant ovarian (OV) cancer with a drug resistance index reaching up to ~ 60 was chosen to evaluate the performance of an efficient gene/drug co-delivery nanocarrier.
31846802	2	39	gly	P-glycoprotein	513:526	arg1	P-gp					529:532	P-gp	529:532	P-gp	529:532	Drug resistance has been associated with the high expression of the multi-drug resistance gene 1 (MDR1), which can encode an efflux transporter known as P-glycoprotein (P-gp) that is located in the cellular membrane.
31846802	2	39	gly	P-glycoprotein	513:526	arg1	P-glycoprotein					513:526	P-glycoprotein	513:526	P-glycoprotein (P-gp) that is located in the cellular membrane	513:574	Drug resistance has been associated with the high expression of the multi-drug resistance gene 1 (MDR1), which can encode an efflux transporter known as P-glycoprotein (P-gp) that is located in the cellular membrane.
31846802	9	40	theme	PTX-only	1830:1837	arg1	system					1839:1844	a PTX-only system	1828:1844	a PTX-only system	1828:1844	Both in vitro and in vivo studies revealed that this dual-delivery system significantly enhanced the therapeutic effect in comparison with that provided by a PTX-only system.
31846802	13	41	theme	OV	2709:2710	arg1	cells					2720:2724	highly chemo-resistant human ovarian (OV) cancer cells	2671:2724	highly chemo-resistant human ovarian (OV) cancer cells	2671:2724	The MDR1-siRNA/PTX co-loaded biomimetic lipid/dextran hybrid nanocarriers demonstrate good capability in overcoming the PTX-resistance in highly chemo-resistant human ovarian (OV) cancer cells both in vitro and in vivo.
31846802	7	42	theme	anionic	1325:1331	arg1	MDR1-siRNA					1333:1342	The anionic MDR1-siRNA	1321:1342	The anionic MDR1-siRNA	1321:1342	The anionic MDR1-siRNA and the hydrophobic drug PTX were respectively loaded into the cationic lipid shell and the hydrophobic internal core of the hybrid nanocarriers.
31846802	2	43	theme	efflux	485:490	arg1	gene					450:453	multi-drug resistance gene 1	428:455	the multi-drug resistance gene 1 (MDR1)	424:462	Drug resistance has been associated with the high expression of the multi-drug resistance gene 1 (MDR1), which can encode an efflux transporter known as P-glycoprotein (P-gp) that is located in the cellular membrane.
31846802	2	43	theme	efflux	485:490	arg1	transporter					492:502	an efflux transporter	482:502	an efflux transporter known as P-glycoprotein (P-gp) that is located in the cellular membrane	482:574	Drug resistance has been associated with the high expression of the multi-drug resistance gene 1 (MDR1), which can encode an efflux transporter known as P-glycoprotein (P-gp) that is located in the cellular membrane.
31846802	12	44	theme	co-deliver	2502:2511	arg1	MDR1-siRNA					2513:2522	co-deliver MDR1-siRNA	2502:2522	co-deliver MDR1-siRNA	2502:2522	STATEMENT OF SIGNIFICANCE: The biomimetic lipid/dextran hybrid nanocarrier with a diameter of ~ 100 nm, which was fabricated via the frame-guided self-assembly of lipid amphiphiles onto the surface of redox-cleavable dextran-based nanogels, provides a model carrier to co-deliver MDR1-siRNA and PTX.
31846802	10	45	theme	gene/chemo	1873:1882	arg1	nanocarriers					1911:1922	gene/chemo co-delivered lipid/dextran nanocarriers	1873:1922	gene/chemo co-delivered lipid/dextran nanocarriers	1873:1922	Thus, the construction of gene/chemo co-delivered lipid/dextran nanocarriers provides a new strategy to inhibit highly drug-resistant tumors both in vitro and in vivo.
31846802	4	46	theme	resistance	811:820	arg1	index					822:826	a drug resistance index	804:826	a drug resistance index reaching up to ~ 60	804:846	Herein, highly paclitaxel (PTX)-resistant ovarian (OV) cancer with a drug resistance index reaching up to ~ 60 was chosen to evaluate the performance of an efficient gene/drug co-delivery nanocarrier.
31846802	2	47	theme	gene	450:453	arg1	expression					410:419	the high expression	401:419	the high expression of the multi-drug resistance gene 1 (MDR1), which can encode an efflux transporter known as P-glycoprotein (P-gp) that is located in the cellular membrane	401:574	Drug resistance has been associated with the high expression of the multi-drug resistance gene 1 (MDR1), which can encode an efflux transporter known as P-glycoprotein (P-gp) that is located in the cellular membrane.
31846802	1	48	theme	in	340:341	arg1	treatments					348:357	in vivo treatments	340:357	in vivo treatments	340:357	While drug resistance has been recognized as the main cause of unsuccessful chemotherapy, the efficient inhibition of highly drug-resistant tumors still remains a significant challenge, especially for in vivo treatments.
31846802	11	49	theme	therapeutic	2207:2217	arg1	nanocarriers					2219:2230	effective therapeutic nanocarriers	2197:2230	effective therapeutic nanocarriers	2197:2230	In addition, this work will contribute toward the development of urgently needed tumor nanotherapy that is able to overcome drug resistance while also offering an unmatched range of effective therapeutic nanocarriers.
31846802	3	50	theme	drugs	655:659	arg1	delivery					601:608	the combined delivery	588:608	the combined delivery of MDR1-inhibited genes and chemotherapeutic drugs	588:659	Therefore, the combined delivery of MDR1-inhibited genes and chemotherapeutic drugs is anticipated to enable the effective inhibition of drug-resistant tumors.
31846802	8	51	from	accumulation	1536:1547	arg1	cells					1559:1563	cells	1559:1563	cells	1559:1563	MDR1-siRNA can knock down MDR1, promoting the accumulation of PTX in cells, and thus is expected to achieve an efficient inhibitory effect against highly PTX-resistant cancer cells.
31846802	5	52	theme	release	1148:1154	arg1	endocytosis					1104:1114	the endocytosis	1100:1114	the endocytosis	1100:1114	Inspired by the self-assembly that occurs in cells and exosomes, we designed a biomimetic lipid/dextran hybrid nanocarrier with a diameter of ~ 100 nm to enhance the endocytosis and the efficiency of drug/gene release within the cells.
31846802	5	52	theme	release	1148:1154	arg1	efficiency					1124:1133	the efficiency	1120:1133	the efficiency of drug/gene release	1120:1154	Inspired by the self-assembly that occurs in cells and exosomes, we designed a biomimetic lipid/dextran hybrid nanocarrier with a diameter of ~ 100 nm to enhance the endocytosis and the efficiency of drug/gene release within the cells.
31846802	5	53	theme	biomimetic	1017:1026	arg1	nanocarrier					1049:1059	a biomimetic lipid/dextran hybrid nanocarrier	1015:1059	a biomimetic lipid/dextran hybrid nanocarrier with a diameter of ~ 100 nm	1015:1087	Inspired by the self-assembly that occurs in cells and exosomes, we designed a biomimetic lipid/dextran hybrid nanocarrier with a diameter of ~ 100 nm to enhance the endocytosis and the efficiency of drug/gene release within the cells.
31846802	3	54	theme	effective	690:698	arg1	inhibition					700:709	the effective inhibition	686:709	the effective inhibition of drug-resistant tumors	686:734	Therefore, the combined delivery of MDR1-inhibited genes and chemotherapeutic drugs is anticipated to enable the effective inhibition of drug-resistant tumors.
31846802	10	55	theme	lipid/dextran	1897:1909	arg1	nanocarriers					1911:1922	gene/chemo co-delivered lipid/dextran nanocarriers	1873:1922	gene/chemo co-delivered lipid/dextran nanocarriers	1873:1922	Thus, the construction of gene/chemo co-delivered lipid/dextran nanocarriers provides a new strategy to inhibit highly drug-resistant tumors both in vitro and in vivo.
31846802	2	56	theme	high	405:408	arg1	expression					410:419	the high expression	401:419	the high expression of the multi-drug resistance gene 1 (MDR1), which can encode an efflux transporter known as P-glycoprotein (P-gp) that is located in the cellular membrane	401:574	Drug resistance has been associated with the high expression of the multi-drug resistance gene 1 (MDR1), which can encode an efflux transporter known as P-glycoprotein (P-gp) that is located in the cellular membrane.
31846802	1	57	theme	drug-resistant	264:277	arg1	tumors					279:284	highly drug-resistant tumors	257:284	highly drug-resistant tumors	257:284	While drug resistance has been recognized as the main cause of unsuccessful chemotherapy, the efficient inhibition of highly drug-resistant tumors still remains a significant challenge, especially for in vivo treatments.
31846802	9	58	dep	in	1677:1678	arg1	vitro					1680:1684	vitro	1680:1684	vitro	1680:1684	Both in vitro and in vivo studies revealed that this dual-delivery system significantly enhanced the therapeutic effect in comparison with that provided by a PTX-only system.
31846802	8	59	theme	inhibitory	1611:1620	arg1	effect					1622:1627	an efficient inhibitory effect	1598:1627	an efficient inhibitory effect against highly PTX-resistant cancer cells	1598:1669	MDR1-siRNA can knock down MDR1, promoting the accumulation of PTX in cells, and thus is expected to achieve an efficient inhibitory effect against highly PTX-resistant cancer cells.
31846802	3	60	theme	tumors	729:734	arg1	inhibition					700:709	the effective inhibition	686:709	the effective inhibition of drug-resistant tumors	686:734	Therefore, the combined delivery of MDR1-inhibited genes and chemotherapeutic drugs is anticipated to enable the effective inhibition of drug-resistant tumors.
31846802	6	61	theme	dextran-based	1297:1309	arg1	nanogels					1311:1318	redox-cleavable dextran-based nanogels	1281:1318	redox-cleavable dextran-based nanogels	1281:1318	This nanocarrier was fabricated via the frame-guided self-assembly of lipid amphiphiles on the surfaces of redox-cleavable dextran-based nanogels.
31846802	13	62	from	PTX-resistance	2653:2666	arg1	cells					2720:2724	highly chemo-resistant human ovarian (OV) cancer cells	2671:2724	highly chemo-resistant human ovarian (OV) cancer cells	2671:2724	The MDR1-siRNA/PTX co-loaded biomimetic lipid/dextran hybrid nanocarriers demonstrate good capability in overcoming the PTX-resistance in highly chemo-resistant human ovarian (OV) cancer cells both in vitro and in vivo.
31846802	12	63	theme	amphiphiles	2402:2412	arg1	self-assembly					2379:2391	the frame-guided self-assembly	2362:2391	the frame-guided self-assembly of lipid amphiphiles	2362:2412	STATEMENT OF SIGNIFICANCE: The biomimetic lipid/dextran hybrid nanocarrier with a diameter of ~ 100 nm, which was fabricated via the frame-guided self-assembly of lipid amphiphiles onto the surface of redox-cleavable dextran-based nanogels, provides a model carrier to co-deliver MDR1-siRNA and PTX.
31846802	10	64	theme	new	1935:1937	arg1	strategy					1939:1946	a new strategy	1933:1946	a new strategy to inhibit highly drug-resistant tumors both in vitro and in vivo	1933:2012	Thus, the construction of gene/chemo co-delivered lipid/dextran nanocarriers provides a new strategy to inhibit highly drug-resistant tumors both in vitro and in vivo.
31846802	7	65	theme	nanocarriers	1476:1487	arg1	core					1457:1460	the hydrophobic internal core	1432:1460	the hydrophobic internal core of the hybrid nanocarriers	1432:1487	The anionic MDR1-siRNA and the hydrophobic drug PTX were respectively loaded into the cationic lipid shell and the hydrophobic internal core of the hybrid nanocarriers.
31846802	7	65	theme	nanocarriers	1476:1487	arg1	shell					1422:1426	the cationic lipid shell	1403:1426	the cationic lipid shell	1403:1426	The anionic MDR1-siRNA and the hydrophobic drug PTX were respectively loaded into the cationic lipid shell and the hydrophobic internal core of the hybrid nanocarriers.
31846802	9	66	theme	in	1677:1678	arg1	studies					1698:1704	Both in vitro and in vivo studies	1672:1704	Both in vitro and in vivo studies	1672:1704	Both in vitro and in vivo studies revealed that this dual-delivery system significantly enhanced the therapeutic effect in comparison with that provided by a PTX-only system.
31846802	8	67	theme	PTX-resistant	1644:1656	arg1	cells					1665:1669	highly PTX-resistant cancer cells	1637:1669	highly PTX-resistant cancer cells	1637:1669	MDR1-siRNA can knock down MDR1, promoting the accumulation of PTX in cells, and thus is expected to achieve an efficient inhibitory effect against highly PTX-resistant cancer cells.
31846802	13	68	theme	hybrid	2587:2592	arg1	nanocarriers					2594:2605	The MDR1-siRNA/PTX co-loaded biomimetic lipid/dextran hybrid nanocarriers	2533:2605	The MDR1-siRNA/PTX co-loaded biomimetic lipid/dextran hybrid nanocarriers	2533:2605	The MDR1-siRNA/PTX co-loaded biomimetic lipid/dextran hybrid nanocarriers demonstrate good capability in overcoming the PTX-resistance in highly chemo-resistant human ovarian (OV) cancer cells both in vitro and in vivo.
31846802	12	69	theme	redox-cleavable	2434:2448	arg1	nanogels					2464:2471	redox-cleavable dextran-based nanogels	2434:2471	redox-cleavable dextran-based nanogels	2434:2471	STATEMENT OF SIGNIFICANCE: The biomimetic lipid/dextran hybrid nanocarrier with a diameter of ~ 100 nm, which was fabricated via the frame-guided self-assembly of lipid amphiphiles onto the surface of redox-cleavable dextran-based nanogels, provides a model carrier to co-deliver MDR1-siRNA and PTX.
31846802	13	70	theme	chemo-resistant	2678:2692	arg1	cells					2720:2724	highly chemo-resistant human ovarian (OV) cancer cells	2671:2724	highly chemo-resistant human ovarian (OV) cancer cells	2671:2724	The MDR1-siRNA/PTX co-loaded biomimetic lipid/dextran hybrid nanocarriers demonstrate good capability in overcoming the PTX-resistance in highly chemo-resistant human ovarian (OV) cancer cells both in vitro and in vivo.
31846802	7	71	theme	internal	1448:1455	arg1	core					1457:1460	the hydrophobic internal core	1432:1460	the hydrophobic internal core of the hybrid nanocarriers	1432:1487	The anionic MDR1-siRNA and the hydrophobic drug PTX were respectively loaded into the cationic lipid shell and the hydrophobic internal core of the hybrid nanocarriers.
31846802	6	72	theme	amphiphiles	1250:1260	arg1	self-assembly					1227:1239	the frame-guided self-assembly	1210:1239	the frame-guided self-assembly of lipid amphiphiles on the surfaces of redox-cleavable dextran-based nanogels	1210:1318	This nanocarrier was fabricated via the frame-guided self-assembly of lipid amphiphiles on the surfaces of redox-cleavable dextran-based nanogels.
31846802	1	73	theme	unsuccessful	202:213	arg1	chemotherapy					215:226	unsuccessful chemotherapy	202:226	unsuccessful chemotherapy	202:226	While drug resistance has been recognized as the main cause of unsuccessful chemotherapy, the efficient inhibition of highly drug-resistant tumors still remains a significant challenge, especially for in vivo treatments.
31846802	13	74	theme	good	2619:2622	arg1	capability					2624:2633	good capability	2619:2633	good capability	2619:2633	The MDR1-siRNA/PTX co-loaded biomimetic lipid/dextran hybrid nanocarriers demonstrate good capability in overcoming the PTX-resistance in highly chemo-resistant human ovarian (OV) cancer cells both in vitro and in vivo.
31846802	13	75	theme	MDR1-siRNA/PTX	2537:2550	arg1	nanocarriers					2594:2605	The MDR1-siRNA/PTX co-loaded biomimetic lipid/dextran hybrid nanocarriers	2533:2605	The MDR1-siRNA/PTX co-loaded biomimetic lipid/dextran hybrid nanocarriers	2533:2605	The MDR1-siRNA/PTX co-loaded biomimetic lipid/dextran hybrid nanocarriers demonstrate good capability in overcoming the PTX-resistance in highly chemo-resistant human ovarian (OV) cancer cells both in vitro and in vivo.
31846802	12	76	dep	STATEMENT	2233:2241	arg1	provides					2474:2481	provides	2474:2481	provides a model carrier to co-deliver MDR1-siRNA and PTX	2474:2530	STATEMENT OF SIGNIFICANCE: The biomimetic lipid/dextran hybrid nanocarrier with a diameter of ~ 100 nm, which was fabricated via the frame-guided self-assembly of lipid amphiphiles onto the surface of redox-cleavable dextran-based nanogels, provides a model carrier to co-deliver MDR1-siRNA and PTX.
31846802	4	77	theme	efficient	893:901	arg1	nanocarrier					925:935	an efficient gene/drug co-delivery nanocarrier	890:935	an efficient gene/drug co-delivery nanocarrier	890:935	Herein, highly paclitaxel (PTX)-resistant ovarian (OV) cancer with a drug resistance index reaching up to ~ 60 was chosen to evaluate the performance of an efficient gene/drug co-delivery nanocarrier.
31846802	4	78	dep	-resistant	768:777	arg1	paclitaxel					752:761	paclitaxel	752:761	paclitaxel	752:761	Herein, highly paclitaxel (PTX)-resistant ovarian (OV) cancer with a drug resistance index reaching up to ~ 60 was chosen to evaluate the performance of an efficient gene/drug co-delivery nanocarrier.
31846802	13	79	theme	biomimetic	2562:2571	arg1	nanocarriers					2594:2605	The MDR1-siRNA/PTX co-loaded biomimetic lipid/dextran hybrid nanocarriers	2533:2605	The MDR1-siRNA/PTX co-loaded biomimetic lipid/dextran hybrid nanocarriers	2533:2605	The MDR1-siRNA/PTX co-loaded biomimetic lipid/dextran hybrid nanocarriers demonstrate good capability in overcoming the PTX-resistance in highly chemo-resistant human ovarian (OV) cancer cells both in vitro and in vivo.
31846802	13	80	theme	ovarian	2700:2706	arg1	cells					2720:2724	highly chemo-resistant human ovarian (OV) cancer cells	2671:2724	highly chemo-resistant human ovarian (OV) cancer cells	2671:2724	The MDR1-siRNA/PTX co-loaded biomimetic lipid/dextran hybrid nanocarriers demonstrate good capability in overcoming the PTX-resistance in highly chemo-resistant human ovarian (OV) cancer cells both in vitro and in vivo.
31846802	3	81	theme	combined	592:599	arg1	delivery					601:608	the combined delivery	588:608	the combined delivery of MDR1-inhibited genes and chemotherapeutic drugs	588:659	Therefore, the combined delivery of MDR1-inhibited genes and chemotherapeutic drugs is anticipated to enable the effective inhibition of drug-resistant tumors.
31846802	1	82	theme	efficient	233:241	arg1	inhibition					243:252	the efficient inhibition	229:252	the efficient inhibition of highly drug-resistant tumors	229:284	While drug resistance has been recognized as the main cause of unsuccessful chemotherapy, the efficient inhibition of highly drug-resistant tumors still remains a significant challenge, especially for in vivo treatments.
31846802	4	83	theme	co-delivery	913:923	arg1	nanocarrier					925:935	an efficient gene/drug co-delivery nanocarrier	890:935	an efficient gene/drug co-delivery nanocarrier	890:935	Herein, highly paclitaxel (PTX)-resistant ovarian (OV) cancer with a drug resistance index reaching up to ~ 60 was chosen to evaluate the performance of an efficient gene/drug co-delivery nanocarrier.
31846802	12	84	theme	lipid	2396:2400	arg1	amphiphiles					2402:2412	lipid amphiphiles	2396:2412	lipid amphiphiles	2396:2412	STATEMENT OF SIGNIFICANCE: The biomimetic lipid/dextran hybrid nanocarrier with a diameter of ~ 100 nm, which was fabricated via the frame-guided self-assembly of lipid amphiphiles onto the surface of redox-cleavable dextran-based nanogels, provides a model carrier to co-deliver MDR1-siRNA and PTX.
31846802	7	85	theme	cationic	1407:1414	arg1	shell					1422:1426	the cationic lipid shell	1403:1426	the cationic lipid shell	1403:1426	The anionic MDR1-siRNA and the hydrophobic drug PTX were respectively loaded into the cationic lipid shell and the hydrophobic internal core of the hybrid nanocarriers.
31846802	5	86	theme	~	1080:1080	arg1	100 nm					1082:1087	~ 100 nm	1080:1087	~ 100 nm	1080:1087	Inspired by the self-assembly that occurs in cells and exosomes, we designed a biomimetic lipid/dextran hybrid nanocarrier with a diameter of ~ 100 nm to enhance the endocytosis and the efficiency of drug/gene release within the cells.
31846802	9	87	with	comparison	1795:1804	arg1	that					1811:1814	that	1811:1814	that	1811:1814	Both in vitro and in vivo studies revealed that this dual-delivery system significantly enhanced the therapeutic effect in comparison with that provided by a PTX-only system.
31846802	1	88	theme	drug	145:148	arg1	cause					193:197	the main cause	184:197	the main cause of unsuccessful chemotherapy	184:226	While drug resistance has been recognized as the main cause of unsuccessful chemotherapy, the efficient inhibition of highly drug-resistant tumors still remains a significant challenge, especially for in vivo treatments.
31846802	1	88	theme	drug	145:148	arg1	resistance					150:159	drug resistance	145:159	drug resistance	145:159	While drug resistance has been recognized as the main cause of unsuccessful chemotherapy, the efficient inhibition of highly drug-resistant tumors still remains a significant challenge, especially for in vivo treatments.
31846802	11	89	theme	needed	2089:2094	arg1	nanotherapy					2102:2112	urgently needed tumor nanotherapy	2080:2112	urgently needed tumor nanotherapy that is able to overcome drug resistance while also offering an unmatched range of effective therapeutic nanocarriers	2080:2230	In addition, this work will contribute toward the development of urgently needed tumor nanotherapy that is able to overcome drug resistance while also offering an unmatched range of effective therapeutic nanocarriers.
31846802	12	90	with	nanocarrier	2296:2306	arg1	diameter					2315:2322	a diameter	2313:2322	a diameter of ~ 100 nm	2313:2334	STATEMENT OF SIGNIFICANCE: The biomimetic lipid/dextran hybrid nanocarrier with a diameter of ~ 100 nm, which was fabricated via the frame-guided self-assembly of lipid amphiphiles onto the surface of redox-cleavable dextran-based nanogels, provides a model carrier to co-deliver MDR1-siRNA and PTX.
31846802	5	91	theme	hybrid	1042:1047	arg1	nanocarrier					1049:1059	a biomimetic lipid/dextran hybrid nanocarrier	1015:1059	a biomimetic lipid/dextran hybrid nanocarrier with a diameter of ~ 100 nm	1015:1087	Inspired by the self-assembly that occurs in cells and exosomes, we designed a biomimetic lipid/dextran hybrid nanocarrier with a diameter of ~ 100 nm to enhance the endocytosis and the efficiency of drug/gene release within the cells.
31846802	3	92	theme	genes	628:632	arg1	delivery					601:608	the combined delivery	588:608	the combined delivery of MDR1-inhibited genes and chemotherapeutic drugs	588:659	Therefore, the combined delivery of MDR1-inhibited genes and chemotherapeutic drugs is anticipated to enable the effective inhibition of drug-resistant tumors.
31846802	2	93	theme	cellular	558:565	arg1	membrane					567:574	the cellular membrane	554:574	the cellular membrane	554:574	Drug resistance has been associated with the high expression of the multi-drug resistance gene 1 (MDR1), which can encode an efflux transporter known as P-glycoprotein (P-gp) that is located in the cellular membrane.
31846802	8	94	theme	down	1511:1514	arg1	MDR1					1516:1519	down MDR1	1511:1519	down MDR1	1511:1519	MDR1-siRNA can knock down MDR1, promoting the accumulation of PTX in cells, and thus is expected to achieve an efficient inhibitory effect against highly PTX-resistant cancer cells.
31846802	12	95	theme	lipid/dextran	2275:2287	arg1	nanocarrier					2296:2306	The biomimetic lipid/dextran hybrid nanocarrier	2260:2306	The biomimetic lipid/dextran hybrid nanocarrier	2260:2306	STATEMENT OF SIGNIFICANCE: The biomimetic lipid/dextran hybrid nanocarrier with a diameter of ~ 100 nm, which was fabricated via the frame-guided self-assembly of lipid amphiphiles onto the surface of redox-cleavable dextran-based nanogels, provides a model carrier to co-deliver MDR1-siRNA and PTX.
31846802	9	96	theme	therapeutic	1773:1783	arg1	effect					1785:1790	the therapeutic effect	1769:1790	the therapeutic effect	1769:1790	Both in vitro and in vivo studies revealed that this dual-delivery system significantly enhanced the therapeutic effect in comparison with that provided by a PTX-only system.
31846802	12	97	theme	100 nm	2329:2334	arg1	diameter					2315:2322	a diameter	2313:2322	a diameter of ~ 100 nm	2313:2334	STATEMENT OF SIGNIFICANCE: The biomimetic lipid/dextran hybrid nanocarrier with a diameter of ~ 100 nm, which was fabricated via the frame-guided self-assembly of lipid amphiphiles onto the surface of redox-cleavable dextran-based nanogels, provides a model carrier to co-deliver MDR1-siRNA and PTX.
31846802	8	98	theme	efficient	1601:1609	arg1	effect					1622:1627	an efficient inhibitory effect	1598:1627	an efficient inhibitory effect against highly PTX-resistant cancer cells	1598:1669	MDR1-siRNA can knock down MDR1, promoting the accumulation of PTX in cells, and thus is expected to achieve an efficient inhibitory effect against highly PTX-resistant cancer cells.
31846802	13	99	theme	cancer	2713:2718	arg1	cells					2720:2724	highly chemo-resistant human ovarian (OV) cancer cells	2671:2724	highly chemo-resistant human ovarian (OV) cancer cells	2671:2724	The MDR1-siRNA/PTX co-loaded biomimetic lipid/dextran hybrid nanocarriers demonstrate good capability in overcoming the PTX-resistance in highly chemo-resistant human ovarian (OV) cancer cells both in vitro and in vivo.
31846802	11	100	theme	tumor	2096:2100	arg1	nanotherapy					2102:2112	urgently needed tumor nanotherapy	2080:2112	urgently needed tumor nanotherapy that is able to overcome drug resistance while also offering an unmatched range of effective therapeutic nanocarriers	2080:2230	In addition, this work will contribute toward the development of urgently needed tumor nanotherapy that is able to overcome drug resistance while also offering an unmatched range of effective therapeutic nanocarriers.
31846802	0	101	theme	tumors	131:136	arg1	inhibition					95:104	the inhibition	91:104	the inhibition of highly drug-resistant tumors	91:136	Gene/paclitaxel co-delivering nanocarriers prepared by framework-induced self-assembly for the inhibition of highly drug-resistant tumors.
31846802	12	102	theme	biomimetic	2264:2273	arg1	nanocarrier					2296:2306	The biomimetic lipid/dextran hybrid nanocarrier	2260:2306	The biomimetic lipid/dextran hybrid nanocarrier	2260:2306	STATEMENT OF SIGNIFICANCE: The biomimetic lipid/dextran hybrid nanocarrier with a diameter of ~ 100 nm, which was fabricated via the frame-guided self-assembly of lipid amphiphiles onto the surface of redox-cleavable dextran-based nanogels, provides a model carrier to co-deliver MDR1-siRNA and PTX.
31846802	4	103	theme	-resistant	768:777	arg1	cancer					792:797	highly paclitaxel (PTX)-resistant ovarian (OV) cancer	745:797	highly paclitaxel (PTX)-resistant ovarian (OV) cancer with a drug resistance index reaching up to ~ 60	745:846	Herein, highly paclitaxel (PTX)-resistant ovarian (OV) cancer with a drug resistance index reaching up to ~ 60 was chosen to evaluate the performance of an efficient gene/drug co-delivery nanocarrier.
31846802	4	103	theme	-resistant	768:777	arg1	OV					788:789	OV	788:789	OV	788:789	Herein, highly paclitaxel (PTX)-resistant ovarian (OV) cancer with a drug resistance index reaching up to ~ 60 was chosen to evaluate the performance of an efficient gene/drug co-delivery nanocarrier.
31846802	5	104	with	nanocarrier	1049:1059	arg1	diameter					1068:1075	a diameter	1066:1075	a diameter of ~ 100 nm	1066:1087	Inspired by the self-assembly that occurs in cells and exosomes, we designed a biomimetic lipid/dextran hybrid nanocarrier with a diameter of ~ 100 nm to enhance the endocytosis and the efficiency of drug/gene release within the cells.
31846802	1	105	dep	in	340:341	arg1	vivo					343:346	vivo	343:346	vivo	343:346	While drug resistance has been recognized as the main cause of unsuccessful chemotherapy, the efficient inhibition of highly drug-resistant tumors still remains a significant challenge, especially for in vivo treatments.
31846802	12	106	theme	hybrid	2289:2294	arg1	nanocarrier					2296:2306	The biomimetic lipid/dextran hybrid nanocarrier	2260:2306	The biomimetic lipid/dextran hybrid nanocarrier	2260:2306	STATEMENT OF SIGNIFICANCE: The biomimetic lipid/dextran hybrid nanocarrier with a diameter of ~ 100 nm, which was fabricated via the frame-guided self-assembly of lipid amphiphiles onto the surface of redox-cleavable dextran-based nanogels, provides a model carrier to co-deliver MDR1-siRNA and PTX.
31846802	0	107	theme	co-delivering	16:28	arg1	nanocarriers					30:41	Gene/paclitaxel co-delivering nanocarriers	0:41	Gene/paclitaxel co-delivering nanocarriers	0:41	Gene/paclitaxel co-delivering nanocarriers prepared by framework-induced self-assembly for the inhibition of highly drug-resistant tumors.
31846802	11	108	theme	effective	2197:2205	arg1	nanocarriers					2219:2230	effective therapeutic nanocarriers	2197:2230	effective therapeutic nanocarriers	2197:2230	In addition, this work will contribute toward the development of urgently needed tumor nanotherapy that is able to overcome drug resistance while also offering an unmatched range of effective therapeutic nanocarriers.
31846802	10	109	theme	co-delivered	1884:1895	arg1	nanocarriers					1911:1922	gene/chemo co-delivered lipid/dextran nanocarriers	1873:1922	gene/chemo co-delivered lipid/dextran nanocarriers	1873:1922	Thus, the construction of gene/chemo co-delivered lipid/dextran nanocarriers provides a new strategy to inhibit highly drug-resistant tumors both in vitro and in vivo.
31846802	9	110	dep	in	1690:1691	arg1	vivo					1693:1696	vivo	1693:1696	vivo	1693:1696	Both in vitro and in vivo studies revealed that this dual-delivery system significantly enhanced the therapeutic effect in comparison with that provided by a PTX-only system.
31846802	2	111	located	located	543:549	arg1	membrane					567:574	the cellular membrane	554:574	the cellular membrane	554:574	Drug resistance has been associated with the high expression of the multi-drug resistance gene 1 (MDR1), which can encode an efflux transporter known as P-glycoprotein (P-gp) that is located in the cellular membrane.
31846802	2	111	located	located	543:549	arg2	P-gp					529:532	P-gp	529:532	P-gp	529:532	Drug resistance has been associated with the high expression of the multi-drug resistance gene 1 (MDR1), which can encode an efflux transporter known as P-glycoprotein (P-gp) that is located in the cellular membrane.
31846802	2	111	located	located	543:549	arg2	P-glycoprotein					513:526	P-glycoprotein	513:526	P-glycoprotein (P-gp) that is located in the cellular membrane	513:574	Drug resistance has been associated with the high expression of the multi-drug resistance gene 1 (MDR1), which can encode an efflux transporter known as P-glycoprotein (P-gp) that is located in the cellular membrane.
31846802	3	112	theme	chemotherapeutic	638:653	arg1	drugs					655:659	chemotherapeutic drugs	638:659	chemotherapeutic drugs	638:659	Therefore, the combined delivery of MDR1-inhibited genes and chemotherapeutic drugs is anticipated to enable the effective inhibition of drug-resistant tumors.
31846802	4	113	theme	drug	806:809	arg1	index					822:826	a drug resistance index	804:826	a drug resistance index reaching up to ~ 60	804:846	Herein, highly paclitaxel (PTX)-resistant ovarian (OV) cancer with a drug resistance index reaching up to ~ 60 was chosen to evaluate the performance of an efficient gene/drug co-delivery nanocarrier.
31846802	2	114	theme	resistance	439:448	arg1	gene					450:453	multi-drug resistance gene 1	428:455	the multi-drug resistance gene 1 (MDR1)	424:462	Drug resistance has been associated with the high expression of the multi-drug resistance gene 1 (MDR1), which can encode an efflux transporter known as P-glycoprotein (P-gp) that is located in the cellular membrane.
31846802	2	114	theme	resistance	439:448	arg1	MDR1					458:461	MDR1	458:461	MDR1	458:461	Drug resistance has been associated with the high expression of the multi-drug resistance gene 1 (MDR1), which can encode an efflux transporter known as P-glycoprotein (P-gp) that is located in the cellular membrane.
31846802	2	114	theme	resistance	439:448	arg1	transporter					492:502	an efflux transporter	482:502	an efflux transporter known as P-glycoprotein (P-gp) that is located in the cellular membrane	482:574	Drug resistance has been associated with the high expression of the multi-drug resistance gene 1 (MDR1), which can encode an efflux transporter known as P-glycoprotein (P-gp) that is located in the cellular membrane.
31846802	5	115	theme	drug/gene	1138:1146	arg1	release					1148:1154	drug/gene release	1138:1154	drug/gene release	1138:1154	Inspired by the self-assembly that occurs in cells and exosomes, we designed a biomimetic lipid/dextran hybrid nanocarrier with a diameter of ~ 100 nm to enhance the endocytosis and the efficiency of drug/gene release within the cells.
31846802	11	116	theme	nanocarriers	2219:2230	arg1	range					2188:2192	an unmatched range	2175:2192	an unmatched range of effective therapeutic nanocarriers	2175:2230	In addition, this work will contribute toward the development of urgently needed tumor nanotherapy that is able to overcome drug resistance while also offering an unmatched range of effective therapeutic nanocarriers.
31846802	12	117	theme	model	2485:2489	arg1	carrier					2491:2497	a model carrier	2483:2497	a model carrier to co-deliver MDR1-siRNA and PTX	2483:2530	STATEMENT OF SIGNIFICANCE: The biomimetic lipid/dextran hybrid nanocarrier with a diameter of ~ 100 nm, which was fabricated via the frame-guided self-assembly of lipid amphiphiles onto the surface of redox-cleavable dextran-based nanogels, provides a model carrier to co-deliver MDR1-siRNA and PTX.
31846802	2	118	from	located	543:549	arg1	membrane					567:574	the cellular membrane	554:574	the cellular membrane	554:574	Drug resistance has been associated with the high expression of the multi-drug resistance gene 1 (MDR1), which can encode an efflux transporter known as P-glycoprotein (P-gp) that is located in the cellular membrane.
31846802	10	119	theme	nanocarriers	1911:1922	arg1	construction					1857:1868	the construction	1853:1868	the construction of gene/chemo co-delivered lipid/dextran nanocarriers	1853:1922	Thus, the construction of gene/chemo co-delivered lipid/dextran nanocarriers provides a new strategy to inhibit highly drug-resistant tumors both in vitro and in vivo.
31846802	2	120	theme	Drug	360:363	arg1	resistance					365:374	Drug resistance	360:374	Drug resistance	360:374	Drug resistance has been associated with the high expression of the multi-drug resistance gene 1 (MDR1), which can encode an efflux transporter known as P-glycoprotein (P-gp) that is located in the cellular membrane.
31846802	12	121	theme	nanogels	2464:2471	arg1	surface					2423:2429	the surface	2419:2429	the surface of redox-cleavable dextran-based nanogels	2419:2471	STATEMENT OF SIGNIFICANCE: The biomimetic lipid/dextran hybrid nanocarrier with a diameter of ~ 100 nm, which was fabricated via the frame-guided self-assembly of lipid amphiphiles onto the surface of redox-cleavable dextran-based nanogels, provides a model carrier to co-deliver MDR1-siRNA and PTX.
31846802	3	122	theme	drug-resistant	714:727	arg1	tumors					729:734	drug-resistant tumors	714:734	drug-resistant tumors	714:734	Therefore, the combined delivery of MDR1-inhibited genes and chemotherapeutic drugs is anticipated to enable the effective inhibition of drug-resistant tumors.
31846802	12	123	theme	dextran-based	2450:2462	arg1	nanogels					2464:2471	redox-cleavable dextran-based nanogels	2434:2471	redox-cleavable dextran-based nanogels	2434:2471	STATEMENT OF SIGNIFICANCE: The biomimetic lipid/dextran hybrid nanocarrier with a diameter of ~ 100 nm, which was fabricated via the frame-guided self-assembly of lipid amphiphiles onto the surface of redox-cleavable dextran-based nanogels, provides a model carrier to co-deliver MDR1-siRNA and PTX.
31846802	13	124	theme	human	2694:2698	arg1	cells					2720:2724	highly chemo-resistant human ovarian (OV) cancer cells	2671:2724	highly chemo-resistant human ovarian (OV) cancer cells	2671:2724	The MDR1-siRNA/PTX co-loaded biomimetic lipid/dextran hybrid nanocarriers demonstrate good capability in overcoming the PTX-resistance in highly chemo-resistant human ovarian (OV) cancer cells both in vitro and in vivo.
31846802	1	125	theme	tumors	279:284	arg1	inhibition					243:252	the efficient inhibition	229:252	the efficient inhibition of highly drug-resistant tumors	229:284	While drug resistance has been recognized as the main cause of unsuccessful chemotherapy, the efficient inhibition of highly drug-resistant tumors still remains a significant challenge, especially for in vivo treatments.
30423312	7	0	theme	phenotyping	1076:1086	arg1	screen					1088:1093	a biochemical phenotyping screen	1062:1093	a biochemical phenotyping screen to identify defects in protein glycosylation that can elucidate mechanisms of disease among NIH UDP patients	1062:1202	In summary, we describe a biochemical phenotyping screen to identify defects in protein glycosylation that can elucidate mechanisms of disease among NIH UDP patients.
30423312	1	1	theme	human	211:215	arg1	biology					217:223	human biology	211:223	human biology	211:223	The National Institutes of Health (NIH) Undiagnosed Diseases Program (UDP) studies rare genetic disorders not only to achieve diagnoses, but to understand human biology.
30423312	6	2	theme	exome	903:907	arg1	sequencing					909:918	whole exome sequencing	897:918	whole exome sequencing	897:918	In fact, whole exome sequencing revealed only a few patients with CDGs, along with several others having disorders indirectly altering glycosylation.
30423312	0	3	from	Glycomics	0:8	arg1	diseases					18:25	rare diseases	13:25	rare diseases	13:25	Glycomics in rare diseases: from diagnosis tomechanism.
30423312	2	4	used	used	311:314	arg2	UDP					307:309	the NIH UDP	299:309	the NIH UDP	299:309	To ascertain the contribution of protein glycosylation to rare diseases, the NIH UDP used mass spectrometry to agnostically identify abnormalities of N-linked and O-linked glycans in plasma and free oligosaccharides in the urine of 207 patients.
30423312	2	5	theme	protein	259:265	arg1	glycosylation					267:279	protein glycosylation	259:279	protein glycosylation	259:279	To ascertain the contribution of protein glycosylation to rare diseases, the NIH UDP used mass spectrometry to agnostically identify abnormalities of N-linked and O-linked glycans in plasma and free oligosaccharides in the urine of 207 patients.
30423312	6	6	theme	whole	897:901	arg1	sequencing					909:918	whole exome sequencing	897:918	whole exome sequencing	897:918	In fact, whole exome sequencing revealed only a few patients with CDGs, along with several others having disorders indirectly altering glycosylation.
30423312	7	7	theme	protein	1118:1124	arg1	glycosylation					1126:1138	protein glycosylation	1118:1138	protein glycosylation	1118:1138	In summary, we describe a biochemical phenotyping screen to identify defects in protein glycosylation that can elucidate mechanisms of disease among NIH UDP patients.
30423312	0	8	from	tomechanism	43:53	arg1	Glycomics					0:8	Glycomics	0:8	Glycomics in rare diseases: from diagnosis tomechanism	0:53	Glycomics in rare diseases: from diagnosis tomechanism.
30423312	5	9	theme	glycosylation	866:878	arg1	CDGs					881:884	CDGs	881:884	CDGs	881:884	Many of these patients may have secondary glycosylation defects, since it is unlikely that they all have congenital disorders of glycosylation (CDGs).
30423312	5	9	theme	glycosylation	866:878	arg1	disorders					853:861	congenital disorders	842:861	congenital disorders of glycosylation (CDGs)	842:885	Many of these patients may have secondary glycosylation defects, since it is unlikely that they all have congenital disorders of glycosylation (CDGs).
30423312	1	10	theme	National	60:67	arg1	Institutes					69:78	The National Institutes	56:78	The National Institutes of Health (NIH) Undiagnosed Diseases Program (UDP)	56:129	The National Institutes of Health (NIH) Undiagnosed Diseases Program (UDP) studies rare genetic disorders not only to achieve diagnoses, but to understand human biology.
30423312	1	11	theme	rare	139:142	arg1	disorders					152:160	rare genetic disorders	139:160	rare genetic disorders	139:160	The National Institutes of Health (NIH) Undiagnosed Diseases Program (UDP) studies rare genetic disorders not only to achieve diagnoses, but to understand human biology.
30423312	2	12	theme	free	420:423	arg1	oligosaccharides					425:440	free oligosaccharides	420:440	free oligosaccharides	420:440	To ascertain the contribution of protein glycosylation to rare diseases, the NIH UDP used mass spectrometry to agnostically identify abnormalities of N-linked and O-linked glycans in plasma and free oligosaccharides in the urine of 207 patients.
30423312	4	13	with	patients	623:630	arg1	abnormalities					637:649	abnormalities	637:649	abnormalities in plasma and/or urine	637:672	Additional evaluation of the fibroblast glycome in 66 patients with abnormalities in plasma and/or urine revealed a consistent glycome phenotype in 83% of these cases.
30423312	1	14	theme	Institutes	69:78	arg1	UDP					126:128	UDP	126:128	UDP	126:128	The National Institutes of Health (NIH) Undiagnosed Diseases Program (UDP) studies rare genetic disorders not only to achieve diagnoses, but to understand human biology.
30423312	1	14	theme	Institutes	69:78	arg1	Program					117:123	The National Institutes of Health (NIH) Undiagnosed Diseases Program	56:123	The National Institutes of Health (NIH) Undiagnosed Diseases Program (UDP)	56:129	The National Institutes of Health (NIH) Undiagnosed Diseases Program (UDP) studies rare genetic disorders not only to achieve diagnoses, but to understand human biology.
30423312	1	15	theme	genetic	144:150	arg1	disorders					152:160	rare genetic disorders	139:160	rare genetic disorders	139:160	The National Institutes of Health (NIH) Undiagnosed Diseases Program (UDP) studies rare genetic disorders not only to achieve diagnoses, but to understand human biology.
30423312	2	16	theme	rare	284:287	arg1	diseases					289:296	rare diseases	284:296	rare diseases	284:296	To ascertain the contribution of protein glycosylation to rare diseases, the NIH UDP used mass spectrometry to agnostically identify abnormalities of N-linked and O-linked glycans in plasma and free oligosaccharides in the urine of 207 patients.
30423312	5	17	contain	have	764:767	arg2	defects					793:799	secondary glycosylation defects	769:799	secondary glycosylation defects	769:799	Many of these patients may have secondary glycosylation defects, since it is unlikely that they all have congenital disorders of glycosylation (CDGs).
30423312	5	17	contain	have	764:767	arg1	Many					737:740	Many	737:740	Many	737:740	Many of these patients may have secondary glycosylation defects, since it is unlikely that they all have congenital disorders of glycosylation (CDGs).
30423312	5	17	contain	have	764:767	arg1	patients					751:758	these patients	745:758	these patients	745:758	Many of these patients may have secondary glycosylation defects, since it is unlikely that they all have congenital disorders of glycosylation (CDGs).
30423312	1	18	theme	Health	83:88	arg1	Diseases					108:115	Health (NIH) Undiagnosed Diseases	83:115	Health (NIH) Undiagnosed Diseases	83:115	The National Institutes of Health (NIH) Undiagnosed Diseases Program (UDP) studies rare genetic disorders not only to achieve diagnoses, but to understand human biology.
30423312	4	19	theme	glycome	609:615	arg1	evaluation					580:589	Additional evaluation	569:589	Additional evaluation of the fibroblast glycome in 66 patients with abnormalities in plasma and/or urine	569:672	Additional evaluation of the fibroblast glycome in 66 patients with abnormalities in plasma and/or urine revealed a consistent glycome phenotype in 83% of these cases.
30423312	7	20	theme	biochemical	1064:1074	arg1	screen					1088:1093	a biochemical phenotyping screen	1062:1093	a biochemical phenotyping screen to identify defects in protein glycosylation that can elucidate mechanisms of disease among NIH UDP patients	1062:1202	In summary, we describe a biochemical phenotyping screen to identify defects in protein glycosylation that can elucidate mechanisms of disease among NIH UDP patients.
30423312	2	21	theme	glycosylation	267:279	arg1	contribution					243:254	the contribution	239:254	the contribution of protein glycosylation to rare diseases	239:296	To ascertain the contribution of protein glycosylation to rare diseases, the NIH UDP used mass spectrometry to agnostically identify abnormalities of N-linked and O-linked glycans in plasma and free oligosaccharides in the urine of 207 patients.
30423312	4	22	from	evaluation	580:589	arg1	patients					623:630	66 patients	620:630	66 patients with abnormalities in plasma and/or urine	620:672	Additional evaluation of the fibroblast glycome in 66 patients with abnormalities in plasma and/or urine revealed a consistent glycome phenotype in 83% of these cases.
30423312	2	23	theme	O-linked	389:396	arg1	glycans					398:404	N-linked and O-linked glycans	376:404	N-linked and O-linked glycans	376:404	To ascertain the contribution of protein glycosylation to rare diseases, the NIH UDP used mass spectrometry to agnostically identify abnormalities of N-linked and O-linked glycans in plasma and free oligosaccharides in the urine of 207 patients.
30423312	2	24	theme	glycans	398:404	arg1	abnormalities					359:371	abnormalities	359:371	abnormalities of N-linked and O-linked glycans in plasma and free oligosaccharides	359:440	To ascertain the contribution of protein glycosylation to rare diseases, the NIH UDP used mass spectrometry to agnostically identify abnormalities of N-linked and O-linked glycans in plasma and free oligosaccharides in the urine of 207 patients.
30423312	3	25	theme	UDP	479:481	arg1	patients					483:490	UDP patients	479:490	UDP patients	479:490	60% of UDP patients had a glycome profile that deviated from control values in at least 1 fluid.
30423312	1	26	theme	NIH	91:93	arg1	Diseases					108:115	Health (NIH) Undiagnosed Diseases	83:115	Health (NIH) Undiagnosed Diseases	83:115	The National Institutes of Health (NIH) Undiagnosed Diseases Program (UDP) studies rare genetic disorders not only to achieve diagnoses, but to understand human biology.
30423312	5	27	theme	secondary	769:777	arg1	defects					793:799	secondary glycosylation defects	769:799	secondary glycosylation defects	769:799	Many of these patients may have secondary glycosylation defects, since it is unlikely that they all have congenital disorders of glycosylation (CDGs).
30423312	4	28	theme	cases	730:734	arg1	cases					730:734	these cases	724:734	these cases	724:734	Additional evaluation of the fibroblast glycome in 66 patients with abnormalities in plasma and/or urine revealed a consistent glycome phenotype in 83% of these cases.
30423312	4	28	theme	cases	730:734	arg1	%					719:719	83%	717:719	83% of these cases	717:734	Additional evaluation of the fibroblast glycome in 66 patients with abnormalities in plasma and/or urine revealed a consistent glycome phenotype in 83% of these cases.
30423312	2	29	theme	N-linked	376:383	arg1	glycans					398:404	N-linked and O-linked glycans	376:404	N-linked and O-linked glycans	376:404	To ascertain the contribution of protein glycosylation to rare diseases, the NIH UDP used mass spectrometry to agnostically identify abnormalities of N-linked and O-linked glycans in plasma and free oligosaccharides in the urine of 207 patients.
30423312	4	30	theme	fibroblast	598:607	arg1	glycome					609:615	the fibroblast glycome	594:615	the fibroblast glycome	594:615	Additional evaluation of the fibroblast glycome in 66 patients with abnormalities in plasma and/or urine revealed a consistent glycome phenotype in 83% of these cases.
30423312	5	31	contain	have	837:840	arg2	disorders					853:861	congenital disorders	842:861	congenital disorders of glycosylation (CDGs)	842:885	Many of these patients may have secondary glycosylation defects, since it is unlikely that they all have congenital disorders of glycosylation (CDGs).
30423312	5	31	contain	have	837:840	arg1	they					828:831	they	828:831	they	828:831	Many of these patients may have secondary glycosylation defects, since it is unlikely that they all have congenital disorders of glycosylation (CDGs).
30423312	5	31	contain	have	837:840	arg2	CDGs					881:884	CDGs	881:884	CDGs	881:884	Many of these patients may have secondary glycosylation defects, since it is unlikely that they all have congenital disorders of glycosylation (CDGs).
30423312	4	32	theme	glycome	696:702	arg1	phenotype					704:712	a consistent glycome phenotype	683:712	a consistent glycome phenotype	683:712	Additional evaluation of the fibroblast glycome in 66 patients with abnormalities in plasma and/or urine revealed a consistent glycome phenotype in 83% of these cases.
30423312	6	33	contain	having	986:991	arg2	disorders					993:1001	disorders	993:1001	disorders indirectly altering glycosylation	993:1035	In fact, whole exome sequencing revealed only a few patients with CDGs, along with several others having disorders indirectly altering glycosylation.
30423312	6	33	contain	having	986:991	arg1	others					979:984	several others	971:984	several others having disorders indirectly altering glycosylation	971:1035	In fact, whole exome sequencing revealed only a few patients with CDGs, along with several others having disorders indirectly altering glycosylation.
30423312	2	34	theme	NIH	303:305	arg1	UDP					307:309	the NIH UDP	299:309	the NIH UDP	299:309	To ascertain the contribution of protein glycosylation to rare diseases, the NIH UDP used mass spectrometry to agnostically identify abnormalities of N-linked and O-linked glycans in plasma and free oligosaccharides in the urine of 207 patients.
30423312	0	35	theme	diagnosis	33:41	arg1	tomechanism					43:53	diagnosis tomechanism	33:53	diagnosis tomechanism	33:53	Glycomics in rare diseases: from diagnosis tomechanism.
30423312	2	36	link	N-linked	376:383	arg1	glycans					398:404	N-linked and O-linked glycans	376:404	N-linked and O-linked glycans	376:404	To ascertain the contribution of protein glycosylation to rare diseases, the NIH UDP used mass spectrometry to agnostically identify abnormalities of N-linked and O-linked glycans in plasma and free oligosaccharides in the urine of 207 patients.
30423312	3	37	theme	control	533:539	arg1	values					541:546	control values	533:546	control values	533:546	60% of UDP patients had a glycome profile that deviated from control values in at least 1 fluid.
30423312	2	38	from	abnormalities	359:371	arg1	plasma					409:414	plasma	409:414	plasma	409:414	To ascertain the contribution of protein glycosylation to rare diseases, the NIH UDP used mass spectrometry to agnostically identify abnormalities of N-linked and O-linked glycans in plasma and free oligosaccharides in the urine of 207 patients.
30423312	2	38	from	abnormalities	359:371	arg1	oligosaccharides					425:440	free oligosaccharides	420:440	free oligosaccharides	420:440	To ascertain the contribution of protein glycosylation to rare diseases, the NIH UDP used mass spectrometry to agnostically identify abnormalities of N-linked and O-linked glycans in plasma and free oligosaccharides in the urine of 207 patients.
30423312	1	39	theme	Undiagnosed	96:106	arg1	Diseases					108:115	Health (NIH) Undiagnosed Diseases	83:115	Health (NIH) Undiagnosed Diseases	83:115	The National Institutes of Health (NIH) Undiagnosed Diseases Program (UDP) studies rare genetic disorders not only to achieve diagnoses, but to understand human biology.
30423312	3	40	contain	had	492:494	arg2	profile					506:512	a glycome profile	496:512	a glycome profile that deviated from control values in at least 1 fluid	496:566	60% of UDP patients had a glycome profile that deviated from control values in at least 1 fluid.
30423312	3	40	contain	had	492:494	arg1	%					474:474	60%	472:474	60% of UDP patients	472:490	60% of UDP patients had a glycome profile that deviated from control values in at least 1 fluid.
30423312	3	40	contain	had	492:494	arg1	patients					483:490	UDP patients	479:490	UDP patients	479:490	60% of UDP patients had a glycome profile that deviated from control values in at least 1 fluid.
30423312	6	41	with	patients	940:947	arg1	CDGs					954:957	CDGs	954:957	CDGs	954:957	In fact, whole exome sequencing revealed only a few patients with CDGs, along with several others having disorders indirectly altering glycosylation.
30423312	7	42	from	defects	1107:1113	arg1	glycosylation					1126:1138	protein glycosylation	1118:1138	protein glycosylation	1118:1138	In summary, we describe a biochemical phenotyping screen to identify defects in protein glycosylation that can elucidate mechanisms of disease among NIH UDP patients.
30423312	1	43	theme	Diseases	108:115	arg1	Institutes					69:78	The National Institutes	56:78	The National Institutes of Health (NIH) Undiagnosed Diseases Program (UDP)	56:129	The National Institutes of Health (NIH) Undiagnosed Diseases Program (UDP) studies rare genetic disorders not only to achieve diagnoses, but to understand human biology.
30423312	3	44	theme	glycome	498:504	arg1	profile					506:512	a glycome profile	496:512	a glycome profile that deviated from control values in at least 1 fluid	496:566	60% of UDP patients had a glycome profile that deviated from control values in at least 1 fluid.
30423312	3	45	theme	patients	483:490	arg1	%					474:474	60%	472:474	60% of UDP patients	472:490	60% of UDP patients had a glycome profile that deviated from control values in at least 1 fluid.
30423312	3	45	theme	patients	483:490	arg1	patients					483:490	UDP patients	479:490	UDP patients	479:490	60% of UDP patients had a glycome profile that deviated from control values in at least 1 fluid.
30423312	2	46	link	O-linked	389:396	arg1	glycans					398:404	N-linked and O-linked glycans	376:404	N-linked and O-linked glycans	376:404	To ascertain the contribution of protein glycosylation to rare diseases, the NIH UDP used mass spectrometry to agnostically identify abnormalities of N-linked and O-linked glycans in plasma and free oligosaccharides in the urine of 207 patients.
30423312	5	47	theme	patients	751:758	arg1	Many					737:740	Many	737:740	Many	737:740	Many of these patients may have secondary glycosylation defects, since it is unlikely that they all have congenital disorders of glycosylation (CDGs).
30423312	5	47	theme	patients	751:758	arg1	patients					751:758	these patients	745:758	these patients	745:758	Many of these patients may have secondary glycosylation defects, since it is unlikely that they all have congenital disorders of glycosylation (CDGs).
30423312	4	48	theme	consistent	685:694	arg1	phenotype					704:712	a consistent glycome phenotype	683:712	a consistent glycome phenotype	683:712	Additional evaluation of the fibroblast glycome in 66 patients with abnormalities in plasma and/or urine revealed a consistent glycome phenotype in 83% of these cases.
30423312	7	49	theme	UDP	1191:1193	arg1	patients					1195:1202	NIH UDP patients	1187:1202	NIH UDP patients	1187:1202	In summary, we describe a biochemical phenotyping screen to identify defects in protein glycosylation that can elucidate mechanisms of disease among NIH UDP patients.
30423312	5	50	theme	congenital	842:851	arg1	CDGs					881:884	CDGs	881:884	CDGs	881:884	Many of these patients may have secondary glycosylation defects, since it is unlikely that they all have congenital disorders of glycosylation (CDGs).
30423312	5	50	theme	congenital	842:851	arg1	disorders					853:861	congenital disorders	842:861	congenital disorders of glycosylation (CDGs)	842:885	Many of these patients may have secondary glycosylation defects, since it is unlikely that they all have congenital disorders of glycosylation (CDGs).
30423312	2	51	theme	patients	462:469	arg1	urine					449:453	the urine	445:453	the urine of 207 patients	445:469	To ascertain the contribution of protein glycosylation to rare diseases, the NIH UDP used mass spectrometry to agnostically identify abnormalities of N-linked and O-linked glycans in plasma and free oligosaccharides in the urine of 207 patients.
30423312	5	52	theme	glycosylation	779:791	arg1	defects					793:799	secondary glycosylation defects	769:799	secondary glycosylation defects	769:799	Many of these patients may have secondary glycosylation defects, since it is unlikely that they all have congenital disorders of glycosylation (CDGs).
30423312	4	53	theme	Additional	569:578	arg1	evaluation					580:589	Additional evaluation	569:589	Additional evaluation of the fibroblast glycome in 66 patients with abnormalities in plasma and/or urine	569:672	Additional evaluation of the fibroblast glycome in 66 patients with abnormalities in plasma and/or urine revealed a consistent glycome phenotype in 83% of these cases.
30423312	7	54	theme	disease	1173:1179	arg1	mechanisms					1159:1168	mechanisms	1159:1168	mechanisms of disease among NIH UDP patients	1159:1202	In summary, we describe a biochemical phenotyping screen to identify defects in protein glycosylation that can elucidate mechanisms of disease among NIH UDP patients.
30423312	6	55	theme	several	971:977	arg1	others					979:984	several others	971:984	several others having disorders indirectly altering glycosylation	971:1035	In fact, whole exome sequencing revealed only a few patients with CDGs, along with several others having disorders indirectly altering glycosylation.
30423312	0	56	theme	rare	13:16	arg1	diseases					18:25	rare diseases	13:25	rare diseases	13:25	Glycomics in rare diseases: from diagnosis tomechanism.
30423312	7	57	theme	NIH	1187:1189	arg1	patients					1195:1202	NIH UDP patients	1187:1202	NIH UDP patients	1187:1202	In summary, we describe a biochemical phenotyping screen to identify defects in protein glycosylation that can elucidate mechanisms of disease among NIH UDP patients.
30423312	2	58	theme	mass	316:319	arg1	spectrometry					321:332	mass spectrometry	316:332	mass spectrometry	316:332	To ascertain the contribution of protein glycosylation to rare diseases, the NIH UDP used mass spectrometry to agnostically identify abnormalities of N-linked and O-linked glycans in plasma and free oligosaccharides in the urine of 207 patients.
30423312	4	59	from	abnormalities	637:649	arg1	plasma					654:659	plasma	654:659	plasma	654:659	Additional evaluation of the fibroblast glycome in 66 patients with abnormalities in plasma and/or urine revealed a consistent glycome phenotype in 83% of these cases.
30423312	4	59	from	abnormalities	637:649	arg1	urine					668:672	urine	668:672	urine	668:672	Additional evaluation of the fibroblast glycome in 66 patients with abnormalities in plasma and/or urine revealed a consistent glycome phenotype in 83% of these cases.
30797114	7	0	theme	gel	1303:1305	arg1	viability					1307:1315	gel viability	1303:1315	gel viability	1303:1315	Furthermore, double gel injections into the same eye yielded better outcomes without compromising gel viability.
30797114	4	1	theme	%	630:630	arg1	weight					647:652	1.5% high molecular weight	627:652	1.5% high molecular weight alginate	627:661	The optimised gels (2 mg/ml collagen, 1.5% high molecular weight alginate and 50,000 cells/gel) could be effectively terminated in vitro (≥20 pg/ml Doxycycline) and in vivo (1 mg/ml oral Doxycycline after 48 h).
30797114	9	2	theme	multiplexed	1514:1524	arg1	dosage					1531:1536	multiplexed GDNF dosage	1514:1536	multiplexed GDNF dosage	1514:1536	The CAC ECT system exhibited mechanical stability, good encapsulation power, cell viability support, multiplexed GDNF dosage, and compatibility with different cell types (HEK293 and ARPE-19) without immunosuppressant, making it an attractive, safe and well-controlled platform for treating various eye diseases.
30797114	0	3	theme	Injectable	0:9	arg1	alginate-collagen					40:56	Injectable cell-encapsulating composite alginate-collagen	0:56	Injectable cell-encapsulating composite alginate-collagen	0:56	Injectable cell-encapsulating composite alginate-collagen platform with inducible termination switch for safer ocular drug delivery.
30797114	5	4	theme	derived	902:908	arg1	GDNF					931:934	GDNF	931:934	GDNF	931:934	Also, they displayed effective proliferation control and continuous delivery of bioactive glial-cell derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.
30797114	5	4	theme	derived	902:908	arg1	factor					923:928	bioactive glial-cell derived neurotrophic factor	881:928	bioactive glial-cell derived neurotrophic factor (GDNF)	881:935	Also, they displayed effective proliferation control and continuous delivery of bioactive glial-cell derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.
30797114	8	5	theme	Retrieved	1318:1326	arg1	gels					1328:1331	Retrieved gels	1318:1331	Retrieved gels	1318:1331	Retrieved gels showed no host-tissue attachment or cell-protrusion 6 months post-implantation.
30797114	5	6	theme	factor	923:928	arg1	control					846:852	effective proliferation control	822:852	effective proliferation control	822:852	Also, they displayed effective proliferation control and continuous delivery of bioactive glial-cell derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.
30797114	5	6	theme	factor	923:928	arg1	delivery					869:876	continuous delivery	858:876	continuous delivery of bioactive glial-cell derived neurotrophic factor (GDNF)	858:935	Also, they displayed effective proliferation control and continuous delivery of bioactive glial-cell derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.
30797114	9	7	theme	GDNF	1526:1529	arg1	dosage					1531:1536	multiplexed GDNF dosage	1514:1536	multiplexed GDNF dosage	1514:1536	The CAC ECT system exhibited mechanical stability, good encapsulation power, cell viability support, multiplexed GDNF dosage, and compatibility with different cell types (HEK293 and ARPE-19) without immunosuppressant, making it an attractive, safe and well-controlled platform for treating various eye diseases.
30797114	1	8	theme	drug	151:154	arg1	delivery					156:163	Effective retinal drug delivery	133:163	Effective retinal drug delivery	133:163	Effective retinal drug delivery remains a challenge for treating vision-threatening diseases.
30797114	8	9	theme	host-tissue	1343:1353	arg1	attachment					1355:1364	no host-tissue attachment	1340:1364	no host-tissue attachment	1340:1364	Retrieved gels showed no host-tissue attachment or cell-protrusion 6 months post-implantation.
30797114	2	10	theme	local	271:275	arg1	delivery					282:289	local drug delivery	271:289	local drug delivery	271:289	Encapsulated-cell therapy (ECT) can provide local drug delivery without repeated invasive injections but is plagued by unsteady performance and biosafety issues.
30797114	5	11	theme	bioactive	881:889	arg1	GDNF					931:934	GDNF	931:934	GDNF	931:934	Also, they displayed effective proliferation control and continuous delivery of bioactive glial-cell derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.
30797114	5	11	theme	bioactive	881:889	arg1	factor					923:928	bioactive glial-cell derived neurotrophic factor	881:928	bioactive glial-cell derived neurotrophic factor (GDNF)	881:935	Also, they displayed effective proliferation control and continuous delivery of bioactive glial-cell derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.
30797114	5	12	theme	significant	945:955	arg1	degradation					961:971	no significant gel degradation	942:971	no significant gel degradation in vitro and in rat vitreous	942:1000	Also, they displayed effective proliferation control and continuous delivery of bioactive glial-cell derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.
30797114	4	13	dep	and	750:752	arg1	Doxycycline					776:786	1 mg/ml oral Doxycycline	763:786	1 mg/ml oral Doxycycline after 48 h	763:797	The optimised gels (2 mg/ml collagen, 1.5% high molecular weight alginate and 50,000 cells/gel) could be effectively terminated in vitro (≥20 pg/ml Doxycycline) and in vivo (1 mg/ml oral Doxycycline after 48 h).
30797114	4	13	dep	and	750:752	arg1	Doxycycline					737:747	≥20 pg/ml Doxycycline	727:747	≥20 pg/ml Doxycycline	727:747	The optimised gels (2 mg/ml collagen, 1.5% high molecular weight alginate and 50,000 cells/gel) could be effectively terminated in vitro (≥20 pg/ml Doxycycline) and in vivo (1 mg/ml oral Doxycycline after 48 h).
30797114	2	14	theme	repeated	299:306	arg1	injections					317:326	repeated invasive injections	299:326	repeated invasive injections	299:326	Encapsulated-cell therapy (ECT) can provide local drug delivery without repeated invasive injections but is plagued by unsteady performance and biosafety issues.
30797114	6	15	theme	therapeutic	1063:1073	arg1	efficacy					1075:1082	therapeutic efficacy	1063:1082	therapeutic efficacy in Royal College of Surgeons rats	1063:1116	Most importantly, intravitreally injected gels demonstrated therapeutic efficacy in Royal College of Surgeons rats with degenerating retina in reducing photoreceptor apoptosis and retina function loss.
30797114	0	16	theme	composite	30:38	arg1	alginate-collagen					40:56	Injectable cell-encapsulating composite alginate-collagen	0:56	Injectable cell-encapsulating composite alginate-collagen	0:56	Injectable cell-encapsulating composite alginate-collagen platform with inducible termination switch for safer ocular drug delivery.
30797114	0	17	theme	safer	105:109	arg1	delivery					123:130	safer ocular drug delivery	105:130	safer ocular drug delivery	105:130	Injectable cell-encapsulating composite alginate-collagen platform with inducible termination switch for safer ocular drug delivery.
30797114	3	18	theme	injectable	398:407	arg1	gel					447:449	an injectable composite alginate-collagen (CAC) ECT gel	395:449	an injectable composite alginate-collagen (CAC) ECT gel with a Tet-on inducible pro-caspase 8 mechanism that acted as an orally-inducible biosafety switch	395:548	Here, an injectable composite alginate-collagen (CAC) ECT gel with a Tet-on inducible pro-caspase 8 mechanism that acted as an orally-inducible biosafety switch was developed for safer drug delivery.
30797114	6	19	from	efficacy	1075:1082	arg1	College					1093:1099	College	1093:1099	College	1093:1099	Most importantly, intravitreally injected gels demonstrated therapeutic efficacy in Royal College of Surgeons rats with degenerating retina in reducing photoreceptor apoptosis and retina function loss.
30797114	0	20	theme	drug	118:121	arg1	delivery					123:130	safer ocular drug delivery	105:130	safer ocular drug delivery	105:130	Injectable cell-encapsulating composite alginate-collagen platform with inducible termination switch for safer ocular drug delivery.
30797114	9	21	dep	types	1577:1581	arg1	HEK293					1584:1589	HEK293	1584:1589	HEK293	1584:1589	The CAC ECT system exhibited mechanical stability, good encapsulation power, cell viability support, multiplexed GDNF dosage, and compatibility with different cell types (HEK293 and ARPE-19) without immunosuppressant, making it an attractive, safe and well-controlled platform for treating various eye diseases.
30797114	9	21	dep	types	1577:1581	arg1	types					1577:1581	different cell types	1562:1581	different cell types (HEK293 and ARPE-19)	1562:1602	The CAC ECT system exhibited mechanical stability, good encapsulation power, cell viability support, multiplexed GDNF dosage, and compatibility with different cell types (HEK293 and ARPE-19) without immunosuppressant, making it an attractive, safe and well-controlled platform for treating various eye diseases.
30797114	9	21	dep	types	1577:1581	arg1	ARPE-19					1595:1601	ARPE-19	1595:1601	ARPE-19	1595:1601	The CAC ECT system exhibited mechanical stability, good encapsulation power, cell viability support, multiplexed GDNF dosage, and compatibility with different cell types (HEK293 and ARPE-19) without immunosuppressant, making it an attractive, safe and well-controlled platform for treating various eye diseases.
30797114	3	22	theme	pro-caspase	475:485	arg1	switch					543:548	an orally-inducible biosafety switch	513:548	an orally-inducible biosafety switch	513:548	Here, an injectable composite alginate-collagen (CAC) ECT gel with a Tet-on inducible pro-caspase 8 mechanism that acted as an orally-inducible biosafety switch was developed for safer drug delivery.
30797114	3	22	theme	pro-caspase	475:485	arg1	mechanism					489:497	a Tet-on inducible pro-caspase 8 mechanism	456:497	a Tet-on inducible pro-caspase 8 mechanism that acted as an orally-inducible biosafety switch	456:548	Here, an injectable composite alginate-collagen (CAC) ECT gel with a Tet-on inducible pro-caspase 8 mechanism that acted as an orally-inducible biosafety switch was developed for safer drug delivery.
30797114	7	23	theme	same	1249:1252	arg1	eye					1254:1256	the same eye	1245:1256	the same eye	1245:1256	Furthermore, double gel injections into the same eye yielded better outcomes without compromising gel viability.
30797114	4	24	dep	gels	603:606	arg1	gels					603:606	The optimised gels	589:606	The optimised gels (2 mg/ml collagen, 1.5% high molecular weight alginate and 50,000 cells/gel)	589:683	The optimised gels (2 mg/ml collagen, 1.5% high molecular weight alginate and 50,000 cells/gel) could be effectively terminated in vitro (≥20 pg/ml Doxycycline) and in vivo (1 mg/ml oral Doxycycline after 48 h).
30797114	4	24	dep	gels	603:606	arg1	collagen					617:624	2 mg/ml collagen	609:624	2 mg/ml collagen	609:624	The optimised gels (2 mg/ml collagen, 1.5% high molecular weight alginate and 50,000 cells/gel) could be effectively terminated in vitro (≥20 pg/ml Doxycycline) and in vivo (1 mg/ml oral Doxycycline after 48 h).
30797114	4	24	dep	gels	603:606	arg1	50,000 cells/gel					667:682	50,000 cells/gel	667:682	50,000 cells/gel	667:682	The optimised gels (2 mg/ml collagen, 1.5% high molecular weight alginate and 50,000 cells/gel) could be effectively terminated in vitro (≥20 pg/ml Doxycycline) and in vivo (1 mg/ml oral Doxycycline after 48 h).
30797114	4	24	dep	gels	603:606	arg1	alginate					654:661	1.5% high molecular weight alginate	627:661	1.5% high molecular weight alginate	627:661	The optimised gels (2 mg/ml collagen, 1.5% high molecular weight alginate and 50,000 cells/gel) could be effectively terminated in vitro (≥20 pg/ml Doxycycline) and in vivo (1 mg/ml oral Doxycycline after 48 h).
30797114	3	25	theme	CAC	438:440	arg1	gel					447:449	an injectable composite alginate-collagen (CAC) ECT gel	395:449	an injectable composite alginate-collagen (CAC) ECT gel with a Tet-on inducible pro-caspase 8 mechanism that acted as an orally-inducible biosafety switch	395:548	Here, an injectable composite alginate-collagen (CAC) ECT gel with a Tet-on inducible pro-caspase 8 mechanism that acted as an orally-inducible biosafety switch was developed for safer drug delivery.
30797114	3	26	theme	safer	568:572	arg1	delivery					579:586	safer drug delivery	568:586	safer drug delivery	568:586	Here, an injectable composite alginate-collagen (CAC) ECT gel with a Tet-on inducible pro-caspase 8 mechanism that acted as an orally-inducible biosafety switch was developed for safer drug delivery.
30797114	4	27	theme	1 mg/ml	763:769	arg1	Doxycycline					776:786	1 mg/ml oral Doxycycline	763:786	1 mg/ml oral Doxycycline after 48 h	763:797	The optimised gels (2 mg/ml collagen, 1.5% high molecular weight alginate and 50,000 cells/gel) could be effectively terminated in vitro (≥20 pg/ml Doxycycline) and in vivo (1 mg/ml oral Doxycycline after 48 h).
30797114	3	28	theme	Tet-on	458:463	arg1	switch					543:548	an orally-inducible biosafety switch	513:548	an orally-inducible biosafety switch	513:548	Here, an injectable composite alginate-collagen (CAC) ECT gel with a Tet-on inducible pro-caspase 8 mechanism that acted as an orally-inducible biosafety switch was developed for safer drug delivery.
30797114	3	28	theme	Tet-on	458:463	arg1	mechanism					489:497	a Tet-on inducible pro-caspase 8 mechanism	456:497	a Tet-on inducible pro-caspase 8 mechanism that acted as an orally-inducible biosafety switch	456:548	Here, an injectable composite alginate-collagen (CAC) ECT gel with a Tet-on inducible pro-caspase 8 mechanism that acted as an orally-inducible biosafety switch was developed for safer drug delivery.
30797114	5	29	theme	proliferation	832:844	arg1	control					846:852	effective proliferation control	822:852	effective proliferation control	822:852	Also, they displayed effective proliferation control and continuous delivery of bioactive glial-cell derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.
30797114	9	30	theme	CAC	1417:1419	arg1	system					1425:1430	The CAC ECT system	1413:1430	The CAC ECT system	1413:1430	The CAC ECT system exhibited mechanical stability, good encapsulation power, cell viability support, multiplexed GDNF dosage, and compatibility with different cell types (HEK293 and ARPE-19) without immunosuppressant, making it an attractive, safe and well-controlled platform for treating various eye diseases.
30797114	5	31	link	derived	902:908	arg1	GDNF					931:934	GDNF	931:934	GDNF	931:934	Also, they displayed effective proliferation control and continuous delivery of bioactive glial-cell derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.
30797114	5	31	link	derived	902:908	arg1	factor					923:928	bioactive glial-cell derived neurotrophic factor	881:928	bioactive glial-cell derived neurotrophic factor (GDNF)	881:935	Also, they displayed effective proliferation control and continuous delivery of bioactive glial-cell derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.
30797114	6	32	theme	Surgeons	1104:1111	arg1	rats					1113:1116	Surgeons rats	1104:1116	Surgeons rats	1104:1116	Most importantly, intravitreally injected gels demonstrated therapeutic efficacy in Royal College of Surgeons rats with degenerating retina in reducing photoreceptor apoptosis and retina function loss.
30797114	3	33	theme	alginate-collagen	419:435	arg1	gel					447:449	an injectable composite alginate-collagen (CAC) ECT gel	395:449	an injectable composite alginate-collagen (CAC) ECT gel with a Tet-on inducible pro-caspase 8 mechanism that acted as an orally-inducible biosafety switch	395:548	Here, an injectable composite alginate-collagen (CAC) ECT gel with a Tet-on inducible pro-caspase 8 mechanism that acted as an orally-inducible biosafety switch was developed for safer drug delivery.
30797114	4	34	theme	optimised	593:601	arg1	gels					603:606	The optimised gels	589:606	The optimised gels (2 mg/ml collagen, 1.5% high molecular weight alginate and 50,000 cells/gel)	589:683	The optimised gels (2 mg/ml collagen, 1.5% high molecular weight alginate and 50,000 cells/gel) could be effectively terminated in vitro (≥20 pg/ml Doxycycline) and in vivo (1 mg/ml oral Doxycycline after 48 h).
30797114	4	34	theme	optimised	593:601	arg1	collagen					617:624	2 mg/ml collagen	609:624	2 mg/ml collagen	609:624	The optimised gels (2 mg/ml collagen, 1.5% high molecular weight alginate and 50,000 cells/gel) could be effectively terminated in vitro (≥20 pg/ml Doxycycline) and in vivo (1 mg/ml oral Doxycycline after 48 h).
30797114	4	34	theme	optimised	593:601	arg1	50,000 cells/gel					667:682	50,000 cells/gel	667:682	50,000 cells/gel	667:682	The optimised gels (2 mg/ml collagen, 1.5% high molecular weight alginate and 50,000 cells/gel) could be effectively terminated in vitro (≥20 pg/ml Doxycycline) and in vivo (1 mg/ml oral Doxycycline after 48 h).
30797114	4	34	theme	optimised	593:601	arg1	alginate					654:661	1.5% high molecular weight alginate	627:661	1.5% high molecular weight alginate	627:661	The optimised gels (2 mg/ml collagen, 1.5% high molecular weight alginate and 50,000 cells/gel) could be effectively terminated in vitro (≥20 pg/ml Doxycycline) and in vivo (1 mg/ml oral Doxycycline after 48 h).
30797114	7	35	theme	gel	1225:1227	arg1	injections					1229:1238	double gel injections	1218:1238	double gel injections into the same eye	1218:1256	Furthermore, double gel injections into the same eye yielded better outcomes without compromising gel viability.
30797114	1	36	theme	Effective	133:141	arg1	delivery					156:163	Effective retinal drug delivery	133:163	Effective retinal drug delivery	133:163	Effective retinal drug delivery remains a challenge for treating vision-threatening diseases.
30797114	5	37	theme	glial-cell	891:900	arg1	GDNF					931:934	GDNF	931:934	GDNF	931:934	Also, they displayed effective proliferation control and continuous delivery of bioactive glial-cell derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.
30797114	5	37	theme	glial-cell	891:900	arg1	factor					923:928	bioactive glial-cell derived neurotrophic factor	881:928	bioactive glial-cell derived neurotrophic factor (GDNF)	881:935	Also, they displayed effective proliferation control and continuous delivery of bioactive glial-cell derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.
30797114	9	38	theme	mechanical	1442:1451	arg1	stability					1453:1461	mechanical stability	1442:1461	mechanical stability	1442:1461	The CAC ECT system exhibited mechanical stability, good encapsulation power, cell viability support, multiplexed GDNF dosage, and compatibility with different cell types (HEK293 and ARPE-19) without immunosuppressant, making it an attractive, safe and well-controlled platform for treating various eye diseases.
30797114	3	39	theme	ECT	443:445	arg1	gel					447:449	an injectable composite alginate-collagen (CAC) ECT gel	395:449	an injectable composite alginate-collagen (CAC) ECT gel with a Tet-on inducible pro-caspase 8 mechanism that acted as an orally-inducible biosafety switch	395:548	Here, an injectable composite alginate-collagen (CAC) ECT gel with a Tet-on inducible pro-caspase 8 mechanism that acted as an orally-inducible biosafety switch was developed for safer drug delivery.
30797114	3	40	theme	biosafety	533:541	arg1	switch					543:548	an orally-inducible biosafety switch	513:548	an orally-inducible biosafety switch	513:548	Here, an injectable composite alginate-collagen (CAC) ECT gel with a Tet-on inducible pro-caspase 8 mechanism that acted as an orally-inducible biosafety switch was developed for safer drug delivery.
30797114	3	40	theme	biosafety	533:541	arg1	mechanism					489:497	a Tet-on inducible pro-caspase 8 mechanism	456:497	a Tet-on inducible pro-caspase 8 mechanism that acted as an orally-inducible biosafety switch	456:548	Here, an injectable composite alginate-collagen (CAC) ECT gel with a Tet-on inducible pro-caspase 8 mechanism that acted as an orally-inducible biosafety switch was developed for safer drug delivery.
30797114	0	41	theme	inducible	72:80	arg1	switch					94:99	inducible termination switch	72:99	inducible termination switch for safer ocular drug delivery	72:130	Injectable cell-encapsulating composite alginate-collagen platform with inducible termination switch for safer ocular drug delivery.
30797114	6	42	theme	retina	1183:1188	arg1	loss					1199:1202	retina function loss	1183:1202	retina function loss	1183:1202	Most importantly, intravitreally injected gels demonstrated therapeutic efficacy in Royal College of Surgeons rats with degenerating retina in reducing photoreceptor apoptosis and retina function loss.
30797114	9	43	theme	encapsulation	1469:1481	arg1	power					1483:1487	good encapsulation power	1464:1487	good encapsulation power	1464:1487	The CAC ECT system exhibited mechanical stability, good encapsulation power, cell viability support, multiplexed GDNF dosage, and compatibility with different cell types (HEK293 and ARPE-19) without immunosuppressant, making it an attractive, safe and well-controlled platform for treating various eye diseases.
30797114	9	44	theme	eye	1711:1713	arg1	diseases					1715:1722	various eye diseases	1703:1722	various eye diseases	1703:1722	The CAC ECT system exhibited mechanical stability, good encapsulation power, cell viability support, multiplexed GDNF dosage, and compatibility with different cell types (HEK293 and ARPE-19) without immunosuppressant, making it an attractive, safe and well-controlled platform for treating various eye diseases.
30797114	4	45	theme	high	632:635	arg1	weight					647:652	1.5% high molecular weight	627:652	1.5% high molecular weight alginate	627:661	The optimised gels (2 mg/ml collagen, 1.5% high molecular weight alginate and 50,000 cells/gel) could be effectively terminated in vitro (≥20 pg/ml Doxycycline) and in vivo (1 mg/ml oral Doxycycline after 48 h).
30797114	8	46	dep	attachment	1355:1364	arg1	post-implantation					1394:1410	post-implantation	1394:1410	post-implantation	1394:1410	Retrieved gels showed no host-tissue attachment or cell-protrusion 6 months post-implantation.
30797114	4	47	theme	weight	647:652	arg1	gels					603:606	The optimised gels	589:606	The optimised gels (2 mg/ml collagen, 1.5% high molecular weight alginate and 50,000 cells/gel)	589:683	The optimised gels (2 mg/ml collagen, 1.5% high molecular weight alginate and 50,000 cells/gel) could be effectively terminated in vitro (≥20 pg/ml Doxycycline) and in vivo (1 mg/ml oral Doxycycline after 48 h).
30797114	4	47	theme	weight	647:652	arg1	alginate					654:661	1.5% high molecular weight alginate	627:661	1.5% high molecular weight alginate	627:661	The optimised gels (2 mg/ml collagen, 1.5% high molecular weight alginate and 50,000 cells/gel) could be effectively terminated in vitro (≥20 pg/ml Doxycycline) and in vivo (1 mg/ml oral Doxycycline after 48 h).
30797114	7	48	theme	better	1266:1271	arg1	outcomes					1273:1280	better outcomes	1266:1280	better outcomes	1266:1280	Furthermore, double gel injections into the same eye yielded better outcomes without compromising gel viability.
30797114	9	49	theme	viability	1495:1503	arg1	support					1505:1511	cell viability support	1490:1511	cell viability support	1490:1511	The CAC ECT system exhibited mechanical stability, good encapsulation power, cell viability support, multiplexed GDNF dosage, and compatibility with different cell types (HEK293 and ARPE-19) without immunosuppressant, making it an attractive, safe and well-controlled platform for treating various eye diseases.
30797114	3	50	with	gel	447:449	arg1	switch					543:548	an orally-inducible biosafety switch	513:548	an orally-inducible biosafety switch	513:548	Here, an injectable composite alginate-collagen (CAC) ECT gel with a Tet-on inducible pro-caspase 8 mechanism that acted as an orally-inducible biosafety switch was developed for safer drug delivery.
30797114	3	50	with	gel	447:449	arg1	mechanism					489:497	a Tet-on inducible pro-caspase 8 mechanism	456:497	a Tet-on inducible pro-caspase 8 mechanism that acted as an orally-inducible biosafety switch	456:548	Here, an injectable composite alginate-collagen (CAC) ECT gel with a Tet-on inducible pro-caspase 8 mechanism that acted as an orally-inducible biosafety switch was developed for safer drug delivery.
30797114	4	51	theme	1.5	627:629	arg1	%					630:630	%	630:630	%	630:630	The optimised gels (2 mg/ml collagen, 1.5% high molecular weight alginate and 50,000 cells/gel) could be effectively terminated in vitro (≥20 pg/ml Doxycycline) and in vivo (1 mg/ml oral Doxycycline after 48 h).
30797114	3	52	theme	drug	574:577	arg1	delivery					579:586	safer drug delivery	568:586	safer drug delivery	568:586	Here, an injectable composite alginate-collagen (CAC) ECT gel with a Tet-on inducible pro-caspase 8 mechanism that acted as an orally-inducible biosafety switch was developed for safer drug delivery.
30797114	1	53	theme	vision-threatening	198:215	arg1	diseases					217:224	vision-threatening diseases	198:224	vision-threatening diseases	198:224	Effective retinal drug delivery remains a challenge for treating vision-threatening diseases.
30797114	5	54	theme	neurotrophic	910:921	arg1	GDNF					931:934	GDNF	931:934	GDNF	931:934	Also, they displayed effective proliferation control and continuous delivery of bioactive glial-cell derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.
30797114	5	54	theme	neurotrophic	910:921	arg1	factor					923:928	bioactive glial-cell derived neurotrophic factor	881:928	bioactive glial-cell derived neurotrophic factor (GDNF)	881:935	Also, they displayed effective proliferation control and continuous delivery of bioactive glial-cell derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.
30797114	2	55	theme	unsteady	346:353	arg1	performance					355:365	unsteady performance	346:365	unsteady performance	346:365	Encapsulated-cell therapy (ECT) can provide local drug delivery without repeated invasive injections but is plagued by unsteady performance and biosafety issues.
30797114	5	56	theme	continuous	858:867	arg1	delivery					869:876	continuous delivery	858:876	continuous delivery of bioactive glial-cell derived neurotrophic factor (GDNF)	858:935	Also, they displayed effective proliferation control and continuous delivery of bioactive glial-cell derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.
30797114	2	57	theme	drug	277:280	arg1	delivery					282:289	local drug delivery	271:289	local drug delivery	271:289	Encapsulated-cell therapy (ECT) can provide local drug delivery without repeated invasive injections but is plagued by unsteady performance and biosafety issues.
30797114	9	58	theme	different	1562:1570	arg1	HEK293					1584:1589	HEK293	1584:1589	HEK293	1584:1589	The CAC ECT system exhibited mechanical stability, good encapsulation power, cell viability support, multiplexed GDNF dosage, and compatibility with different cell types (HEK293 and ARPE-19) without immunosuppressant, making it an attractive, safe and well-controlled platform for treating various eye diseases.
30797114	9	58	theme	different	1562:1570	arg1	types					1577:1581	different cell types	1562:1581	different cell types (HEK293 and ARPE-19)	1562:1602	The CAC ECT system exhibited mechanical stability, good encapsulation power, cell viability support, multiplexed GDNF dosage, and compatibility with different cell types (HEK293 and ARPE-19) without immunosuppressant, making it an attractive, safe and well-controlled platform for treating various eye diseases.
30797114	9	58	theme	different	1562:1570	arg1	ARPE-19					1595:1601	ARPE-19	1595:1601	ARPE-19	1595:1601	The CAC ECT system exhibited mechanical stability, good encapsulation power, cell viability support, multiplexed GDNF dosage, and compatibility with different cell types (HEK293 and ARPE-19) without immunosuppressant, making it an attractive, safe and well-controlled platform for treating various eye diseases.
30797114	1	59	theme	retinal	143:149	arg1	delivery					156:163	Effective retinal drug delivery	133:163	Effective retinal drug delivery	133:163	Effective retinal drug delivery remains a challenge for treating vision-threatening diseases.
30797114	2	60	theme	invasive	308:315	arg1	injections					317:326	repeated invasive injections	299:326	repeated invasive injections	299:326	Encapsulated-cell therapy (ECT) can provide local drug delivery without repeated invasive injections but is plagued by unsteady performance and biosafety issues.
30797114	5	61	theme	gel	957:959	arg1	degradation					961:971	no significant gel degradation	942:971	no significant gel degradation in vitro and in rat vitreous	942:1000	Also, they displayed effective proliferation control and continuous delivery of bioactive glial-cell derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.
30797114	8	62	theme	cell-protrusion	1369:1383	arg1	months					1387:1392	cell-protrusion 6 months	1369:1392	cell-protrusion 6 months	1369:1392	Retrieved gels showed no host-tissue attachment or cell-protrusion 6 months post-implantation.
30797114	0	63	theme	ocular	111:116	arg1	delivery					123:130	safer ocular drug delivery	105:130	safer ocular drug delivery	105:130	Injectable cell-encapsulating composite alginate-collagen platform with inducible termination switch for safer ocular drug delivery.
30797114	6	64	theme	injected	1036:1043	arg1	gels					1045:1048	intravitreally injected gels	1021:1048	intravitreally injected gels	1021:1048	Most importantly, intravitreally injected gels demonstrated therapeutic efficacy in Royal College of Surgeons rats with degenerating retina in reducing photoreceptor apoptosis and retina function loss.
30797114	4	65	theme	≥20 pg/ml	727:735	arg1	Doxycycline					737:747	≥20 pg/ml Doxycycline	727:747	≥20 pg/ml Doxycycline	727:747	The optimised gels (2 mg/ml collagen, 1.5% high molecular weight alginate and 50,000 cells/gel) could be effectively terminated in vitro (≥20 pg/ml Doxycycline) and in vivo (1 mg/ml oral Doxycycline after 48 h).
30797114	5	66	theme	rat	989:991	arg1	vitreous					993:1000	rat vitreous	989:1000	rat vitreous	989:1000	Also, they displayed effective proliferation control and continuous delivery of bioactive glial-cell derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.
30797114	3	67	theme	composite	409:417	arg1	gel					447:449	an injectable composite alginate-collagen (CAC) ECT gel	395:449	an injectable composite alginate-collagen (CAC) ECT gel with a Tet-on inducible pro-caspase 8 mechanism that acted as an orally-inducible biosafety switch	395:548	Here, an injectable composite alginate-collagen (CAC) ECT gel with a Tet-on inducible pro-caspase 8 mechanism that acted as an orally-inducible biosafety switch was developed for safer drug delivery.
30797114	2	68	theme	biosafety	371:379	arg1	issues					381:386	biosafety issues	371:386	biosafety issues	371:386	Encapsulated-cell therapy (ECT) can provide local drug delivery without repeated invasive injections but is plagued by unsteady performance and biosafety issues.
30797114	5	69	theme	effective	822:830	arg1	control					846:852	effective proliferation control	822:852	effective proliferation control	822:852	Also, they displayed effective proliferation control and continuous delivery of bioactive glial-cell derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.
30797114	9	70	theme	attractive	1644:1653	arg1	platform					1681:1688	an attractive, safe and well-controlled platform	1641:1688	an attractive, safe and well-controlled platform for treating various eye diseases	1641:1722	The CAC ECT system exhibited mechanical stability, good encapsulation power, cell viability support, multiplexed GDNF dosage, and compatibility with different cell types (HEK293 and ARPE-19) without immunosuppressant, making it an attractive, safe and well-controlled platform for treating various eye diseases.
30797114	3	71	theme	inducible	465:473	arg1	switch					543:548	an orally-inducible biosafety switch	513:548	an orally-inducible biosafety switch	513:548	Here, an injectable composite alginate-collagen (CAC) ECT gel with a Tet-on inducible pro-caspase 8 mechanism that acted as an orally-inducible biosafety switch was developed for safer drug delivery.
30797114	3	71	theme	inducible	465:473	arg1	mechanism					489:497	a Tet-on inducible pro-caspase 8 mechanism	456:497	a Tet-on inducible pro-caspase 8 mechanism that acted as an orally-inducible biosafety switch	456:548	Here, an injectable composite alginate-collagen (CAC) ECT gel with a Tet-on inducible pro-caspase 8 mechanism that acted as an orally-inducible biosafety switch was developed for safer drug delivery.
30797114	7	72	theme	double	1218:1223	arg1	injections					1229:1238	double gel injections	1218:1238	double gel injections into the same eye	1218:1256	Furthermore, double gel injections into the same eye yielded better outcomes without compromising gel viability.
30797114	9	73	theme	ECT	1421:1423	arg1	system					1425:1430	The CAC ECT system	1413:1430	The CAC ECT system	1413:1430	The CAC ECT system exhibited mechanical stability, good encapsulation power, cell viability support, multiplexed GDNF dosage, and compatibility with different cell types (HEK293 and ARPE-19) without immunosuppressant, making it an attractive, safe and well-controlled platform for treating various eye diseases.
30797114	9	74	theme	safe	1656:1659	arg1	platform					1681:1688	an attractive, safe and well-controlled platform	1641:1688	an attractive, safe and well-controlled platform for treating various eye diseases	1641:1722	The CAC ECT system exhibited mechanical stability, good encapsulation power, cell viability support, multiplexed GDNF dosage, and compatibility with different cell types (HEK293 and ARPE-19) without immunosuppressant, making it an attractive, safe and well-controlled platform for treating various eye diseases.
30797114	4	75	theme	2 mg/ml	609:615	arg1	gels					603:606	The optimised gels	589:606	The optimised gels (2 mg/ml collagen, 1.5% high molecular weight alginate and 50,000 cells/gel)	589:683	The optimised gels (2 mg/ml collagen, 1.5% high molecular weight alginate and 50,000 cells/gel) could be effectively terminated in vitro (≥20 pg/ml Doxycycline) and in vivo (1 mg/ml oral Doxycycline after 48 h).
30797114	4	75	theme	2 mg/ml	609:615	arg1	collagen					617:624	2 mg/ml collagen	609:624	2 mg/ml collagen	609:624	The optimised gels (2 mg/ml collagen, 1.5% high molecular weight alginate and 50,000 cells/gel) could be effectively terminated in vitro (≥20 pg/ml Doxycycline) and in vivo (1 mg/ml oral Doxycycline after 48 h).
30797114	2	76	theme	Encapsulated-cell	227:243	arg1	ECT					254:256	ECT	254:256	ECT	254:256	Encapsulated-cell therapy (ECT) can provide local drug delivery without repeated invasive injections but is plagued by unsteady performance and biosafety issues.
30797114	2	76	theme	Encapsulated-cell	227:243	arg1	therapy					245:251	Encapsulated-cell therapy	227:251	Encapsulated-cell therapy (ECT)	227:257	Encapsulated-cell therapy (ECT) can provide local drug delivery without repeated invasive injections but is plagued by unsteady performance and biosafety issues.
30797114	4	77	theme	oral	771:774	arg1	Doxycycline					776:786	1 mg/ml oral Doxycycline	763:786	1 mg/ml oral Doxycycline after 48 h	763:797	The optimised gels (2 mg/ml collagen, 1.5% high molecular weight alginate and 50,000 cells/gel) could be effectively terminated in vitro (≥20 pg/ml Doxycycline) and in vivo (1 mg/ml oral Doxycycline after 48 h).
30797114	9	78	theme	well-controlled	1665:1679	arg1	platform					1681:1688	an attractive, safe and well-controlled platform	1641:1688	an attractive, safe and well-controlled platform for treating various eye diseases	1641:1722	The CAC ECT system exhibited mechanical stability, good encapsulation power, cell viability support, multiplexed GDNF dosage, and compatibility with different cell types (HEK293 and ARPE-19) without immunosuppressant, making it an attractive, safe and well-controlled platform for treating various eye diseases.
30797114	0	79	theme	termination	82:92	arg1	switch					94:99	inducible termination switch	72:99	inducible termination switch for safer ocular drug delivery	72:130	Injectable cell-encapsulating composite alginate-collagen platform with inducible termination switch for safer ocular drug delivery.
30797114	6	80	theme	function	1190:1197	arg1	loss					1199:1202	retina function loss	1183:1202	retina function loss	1183:1202	Most importantly, intravitreally injected gels demonstrated therapeutic efficacy in Royal College of Surgeons rats with degenerating retina in reducing photoreceptor apoptosis and retina function loss.
30797114	9	81	theme	good	1464:1467	arg1	power					1483:1487	good encapsulation power	1464:1487	good encapsulation power	1464:1487	The CAC ECT system exhibited mechanical stability, good encapsulation power, cell viability support, multiplexed GDNF dosage, and compatibility with different cell types (HEK293 and ARPE-19) without immunosuppressant, making it an attractive, safe and well-controlled platform for treating various eye diseases.
30797114	9	82	theme	various	1703:1709	arg1	diseases					1715:1722	various eye diseases	1703:1722	various eye diseases	1703:1722	The CAC ECT system exhibited mechanical stability, good encapsulation power, cell viability support, multiplexed GDNF dosage, and compatibility with different cell types (HEK293 and ARPE-19) without immunosuppressant, making it an attractive, safe and well-controlled platform for treating various eye diseases.
30797114	4	83	theme	molecular	637:645	arg1	weight					647:652	1.5% high molecular weight	627:652	1.5% high molecular weight alginate	627:661	The optimised gels (2 mg/ml collagen, 1.5% high molecular weight alginate and 50,000 cells/gel) could be effectively terminated in vitro (≥20 pg/ml Doxycycline) and in vivo (1 mg/ml oral Doxycycline after 48 h).
30797114	3	84	theme	orally-inducible	516:531	arg1	switch					543:548	an orally-inducible biosafety switch	513:548	an orally-inducible biosafety switch	513:548	Here, an injectable composite alginate-collagen (CAC) ECT gel with a Tet-on inducible pro-caspase 8 mechanism that acted as an orally-inducible biosafety switch was developed for safer drug delivery.
30797114	3	84	theme	orally-inducible	516:531	arg1	mechanism					489:497	a Tet-on inducible pro-caspase 8 mechanism	456:497	a Tet-on inducible pro-caspase 8 mechanism that acted as an orally-inducible biosafety switch	456:548	Here, an injectable composite alginate-collagen (CAC) ECT gel with a Tet-on inducible pro-caspase 8 mechanism that acted as an orally-inducible biosafety switch was developed for safer drug delivery.
30797114	9	85	theme	cell	1572:1575	arg1	HEK293					1584:1589	HEK293	1584:1589	HEK293	1584:1589	The CAC ECT system exhibited mechanical stability, good encapsulation power, cell viability support, multiplexed GDNF dosage, and compatibility with different cell types (HEK293 and ARPE-19) without immunosuppressant, making it an attractive, safe and well-controlled platform for treating various eye diseases.
30797114	9	85	theme	cell	1572:1575	arg1	types					1577:1581	different cell types	1562:1581	different cell types (HEK293 and ARPE-19)	1562:1602	The CAC ECT system exhibited mechanical stability, good encapsulation power, cell viability support, multiplexed GDNF dosage, and compatibility with different cell types (HEK293 and ARPE-19) without immunosuppressant, making it an attractive, safe and well-controlled platform for treating various eye diseases.
30797114	9	85	theme	cell	1572:1575	arg1	ARPE-19					1595:1601	ARPE-19	1595:1601	ARPE-19	1595:1601	The CAC ECT system exhibited mechanical stability, good encapsulation power, cell viability support, multiplexed GDNF dosage, and compatibility with different cell types (HEK293 and ARPE-19) without immunosuppressant, making it an attractive, safe and well-controlled platform for treating various eye diseases.
30797114	6	86	theme	photoreceptor	1155:1167	arg1	apoptosis					1169:1177	photoreceptor apoptosis	1155:1177	photoreceptor apoptosis	1155:1177	Most importantly, intravitreally injected gels demonstrated therapeutic efficacy in Royal College of Surgeons rats with degenerating retina in reducing photoreceptor apoptosis and retina function loss.
30797114	0	87	theme	cell-encapsulating	11:28	arg1	alginate-collagen					40:56	Injectable cell-encapsulating composite alginate-collagen	0:56	Injectable cell-encapsulating composite alginate-collagen	0:56	Injectable cell-encapsulating composite alginate-collagen platform with inducible termination switch for safer ocular drug delivery.
30797114	9	88	theme	cell	1490:1493	arg1	support					1505:1511	cell viability support	1490:1511	cell viability support	1490:1511	The CAC ECT system exhibited mechanical stability, good encapsulation power, cell viability support, multiplexed GDNF dosage, and compatibility with different cell types (HEK293 and ARPE-19) without immunosuppressant, making it an attractive, safe and well-controlled platform for treating various eye diseases.
31226699	2	0	dep	in	330:331	arg1	situ					333:336	situ	333:336	situ	333:336	To this end, we developed in situ gelling hydrogels from low-fouling poly(carboxybetaine-co-maleimide) (pCBM) random copolymers and thiolated hyaluronic acid (HA-SH).
31226699	0	1	theme	cell	96:99	arg1	encapsulation					101:113	cell encapsulation	96:113	cell encapsulation	96:113	Tunable degradation of low-fouling carboxybetaine-hyaluronic acid hydrogels for applications in cell encapsulation.
31226699	3	2	theme	pCBM	546:549	arg1	%					591:591	4, 11, and 16 maleimide mol%	564:591	4, 11, and 16 maleimide mol%	564:591	pCBM-HA hydrogel enzymatic degradation rates were tuned 5 fold by altering pCBM composition (4, 11, and 16 maleimide mol%) and 2.3 fold by HA-SH concentration (1-2 wt%).
31226699	3	2	theme	pCBM	546:549	arg1	composition					551:561	pCBM composition	546:561	pCBM composition (4, 11, and 16 maleimide mol%)	546:592	pCBM-HA hydrogel enzymatic degradation rates were tuned 5 fold by altering pCBM composition (4, 11, and 16 maleimide mol%) and 2.3 fold by HA-SH concentration (1-2 wt%).
31226699	3	3	theme	degradation	498:508	arg1	rates					510:514	pCBM-HA hydrogel enzymatic degradation rates	471:514	pCBM-HA hydrogel enzymatic degradation rates	471:514	pCBM-HA hydrogel enzymatic degradation rates were tuned 5 fold by altering pCBM composition (4, 11, and 16 maleimide mol%) and 2.3 fold by HA-SH concentration (1-2 wt%).
31226699	7	4	with	vehicle	1167:1173	arg1	rates					1200:1204	tunable degradation rates	1180:1204	tunable degradation rates	1180:1204	Low-fouling pCBM-HA hydrogels provide a vehicle with tunable degradation rates and biochemical environments for encapsulation applications in cell adoptive therapies.
31226699	7	4	with	vehicle	1167:1173	arg1	environments					1222:1233	biochemical environments	1210:1233	biochemical environments	1210:1233	Low-fouling pCBM-HA hydrogels provide a vehicle with tunable degradation rates and biochemical environments for encapsulation applications in cell adoptive therapies.
31226699	7	5	theme	adoptive	1274:1281	arg1	therapies					1283:1291	cell adoptive therapies	1269:1291	cell adoptive therapies	1269:1291	Low-fouling pCBM-HA hydrogels provide a vehicle with tunable degradation rates and biochemical environments for encapsulation applications in cell adoptive therapies.
31226699	2	6	theme	in	330:331	arg1	hydrogels					346:354	in situ gelling hydrogels	330:354	in situ gelling hydrogels from low-fouling poly(carboxybetaine-co-maleimide) (pCBM) random copolymers and thiolated hyaluronic acid (HA-SH)	330:468	To this end, we developed in situ gelling hydrogels from low-fouling poly(carboxybetaine-co-maleimide) (pCBM) random copolymers and thiolated hyaluronic acid (HA-SH).
31226699	4	7	theme	adsorbed	706:713	arg1	cm-2					722:725	adsorbed ∼20 μg cm-2	706:725	BSA; adsorbed ∼20 μg cm-2	701:725	pCBM-HA gels were low-fouling towards bovine serum albumin (BSA; adsorbed ∼20 μg cm-2) and resisted fibroblast adhesion.
31226699	4	8	theme	bovine	679:684	arg1	albumin					692:698	bovine serum albumin	679:698	bovine serum albumin (BSA; adsorbed ∼20 μg cm-2)	679:726	pCBM-HA gels were low-fouling towards bovine serum albumin (BSA; adsorbed ∼20 μg cm-2) and resisted fibroblast adhesion.
31226699	4	8	theme	bovine	679:684	arg1	BSA					701:703	BSA	701:703	BSA; adsorbed ∼20 μg cm-2	701:725	pCBM-HA gels were low-fouling towards bovine serum albumin (BSA; adsorbed ∼20 μg cm-2) and resisted fibroblast adhesion.
31226699	5	9	theme	%	885:885	arg1	decrease					887:894	39% decrease	883:894	39% decrease in circularity	883:909	To control pCBM-HA bioactivity, the cell adhesive peptide CGRGDS was immobilized on pCBM to promote fibroblast adhesion (39% decrease in circularity), which increased metabolic activity by ∼50%.
31226699	7	10	theme	Low-fouling	1127:1137	arg1	hydrogels					1147:1155	Low-fouling pCBM-HA hydrogels	1127:1155	Low-fouling pCBM-HA hydrogels	1127:1155	Low-fouling pCBM-HA hydrogels provide a vehicle with tunable degradation rates and biochemical environments for encapsulation applications in cell adoptive therapies.
31226699	1	11	contain	have	198:201	arg2	potential					207:215	the potential to improve adoptive cell therapies for cancer immunotherapy and regenerative medicine	203:301	the potential to improve adoptive cell therapies for cancer immunotherapy and regenerative medicine	203:301	Low-fouling hydrogels with tunable degradation rates and biochemical environments have the potential to improve adoptive cell therapies for cancer immunotherapy and regenerative medicine.
31226699	1	11	contain	have	198:201	arg1	hydrogels					128:136	Low-fouling hydrogels	116:136	Low-fouling hydrogels with tunable degradation rates and biochemical environments	116:196	Low-fouling hydrogels with tunable degradation rates and biochemical environments have the potential to improve adoptive cell therapies for cancer immunotherapy and regenerative medicine.
31226699	6	12	theme	T	1034:1034	arg1	cells					1036:1040	encapsulated T cells	1021:1040	encapsulated T cells	1021:1040	pCBM-HA modified with CGRGDS enhanced the metabolic activity of encapsulated T cells by ∼21% compared to gels without HA, indicating their potential for immunotherapies.
31226699	4	13	theme	resisted	732:739	arg1	adhesion					752:759	resisted fibroblast adhesion	732:759	resisted fibroblast adhesion	732:759	pCBM-HA gels were low-fouling towards bovine serum albumin (BSA; adsorbed ∼20 μg cm-2) and resisted fibroblast adhesion.
31226699	2	14	theme	thiolated	436:444	arg1	acid					457:460	thiolated hyaluronic acid	436:460	thiolated hyaluronic acid (HA-SH)	436:468	To this end, we developed in situ gelling hydrogels from low-fouling poly(carboxybetaine-co-maleimide) (pCBM) random copolymers and thiolated hyaluronic acid (HA-SH).
31226699	2	14	theme	thiolated	436:444	arg1	HA-SH					463:467	HA-SH	463:467	HA-SH	463:467	To this end, we developed in situ gelling hydrogels from low-fouling poly(carboxybetaine-co-maleimide) (pCBM) random copolymers and thiolated hyaluronic acid (HA-SH).
31226699	7	15	theme	pCBM-HA	1139:1145	arg1	hydrogels					1147:1155	Low-fouling pCBM-HA hydrogels	1127:1155	Low-fouling pCBM-HA hydrogels	1127:1155	Low-fouling pCBM-HA hydrogels provide a vehicle with tunable degradation rates and biochemical environments for encapsulation applications in cell adoptive therapies.
31226699	5	16	theme	fibroblast	862:871	arg1	adhesion					873:880	fibroblast adhesion	862:880	fibroblast adhesion (39% decrease in circularity)	862:910	To control pCBM-HA bioactivity, the cell adhesive peptide CGRGDS was immobilized on pCBM to promote fibroblast adhesion (39% decrease in circularity), which increased metabolic activity by ∼50%.
31226699	5	17	theme	pCBM-HA	773:779	arg1	bioactivity					781:791	pCBM-HA bioactivity	773:791	pCBM-HA bioactivity	773:791	To control pCBM-HA bioactivity, the cell adhesive peptide CGRGDS was immobilized on pCBM to promote fibroblast adhesion (39% decrease in circularity), which increased metabolic activity by ∼50%.
31226699	6	18	theme	cells	1036:1040	arg1	activity					1009:1016	the metabolic activity	995:1016	the metabolic activity of encapsulated T cells	995:1040	pCBM-HA modified with CGRGDS enhanced the metabolic activity of encapsulated T cells by ∼21% compared to gels without HA, indicating their potential for immunotherapies.
31226699	3	19	theme	hydrogel	479:486	arg1	rates					510:514	pCBM-HA hydrogel enzymatic degradation rates	471:514	pCBM-HA hydrogel enzymatic degradation rates	471:514	pCBM-HA hydrogel enzymatic degradation rates were tuned 5 fold by altering pCBM composition (4, 11, and 16 maleimide mol%) and 2.3 fold by HA-SH concentration (1-2 wt%).
31226699	1	20	with	hydrogels	128:136	arg1	rates					163:167	tunable degradation rates	143:167	tunable degradation rates	143:167	Low-fouling hydrogels with tunable degradation rates and biochemical environments have the potential to improve adoptive cell therapies for cancer immunotherapy and regenerative medicine.
31226699	1	20	with	hydrogels	128:136	arg1	environments					185:196	biochemical environments	173:196	biochemical environments	173:196	Low-fouling hydrogels with tunable degradation rates and biochemical environments have the potential to improve adoptive cell therapies for cancer immunotherapy and regenerative medicine.
31226699	5	21	theme	peptide	812:818	arg1	CGRGDS					820:825	the cell adhesive peptide CGRGDS	794:825	the cell adhesive peptide CGRGDS	794:825	To control pCBM-HA bioactivity, the cell adhesive peptide CGRGDS was immobilized on pCBM to promote fibroblast adhesion (39% decrease in circularity), which increased metabolic activity by ∼50%.
31226699	3	22	theme	maleimide	578:586	arg1	%					591:591	4, 11, and 16 maleimide mol%	564:591	4, 11, and 16 maleimide mol%	564:591	pCBM-HA hydrogel enzymatic degradation rates were tuned 5 fold by altering pCBM composition (4, 11, and 16 maleimide mol%) and 2.3 fold by HA-SH concentration (1-2 wt%).
31226699	3	22	theme	maleimide	578:586	arg1	composition					551:561	pCBM composition	546:561	pCBM composition (4, 11, and 16 maleimide mol%)	546:592	pCBM-HA hydrogel enzymatic degradation rates were tuned 5 fold by altering pCBM composition (4, 11, and 16 maleimide mol%) and 2.3 fold by HA-SH concentration (1-2 wt%).
31226699	0	23	theme	Tunable	0:6	arg1	degradation					8:18	Tunable degradation	0:18	Tunable degradation of low-fouling carboxybetaine-hyaluronic acid	0:64	Tunable degradation of low-fouling carboxybetaine-hyaluronic acid hydrogels for applications in cell encapsulation.
31226699	1	24	theme	Low-fouling	116:126	arg1	hydrogels					128:136	Low-fouling hydrogels	116:136	Low-fouling hydrogels with tunable degradation rates and biochemical environments	116:196	Low-fouling hydrogels with tunable degradation rates and biochemical environments have the potential to improve adoptive cell therapies for cancer immunotherapy and regenerative medicine.
31226699	7	25	theme	cell	1269:1272	arg1	therapies					1283:1291	cell adoptive therapies	1269:1291	cell adoptive therapies	1269:1291	Low-fouling pCBM-HA hydrogels provide a vehicle with tunable degradation rates and biochemical environments for encapsulation applications in cell adoptive therapies.
31226699	1	26	theme	adoptive	228:235	arg1	therapies					242:250	adoptive cell therapies	228:250	adoptive cell therapies for cancer immunotherapy and regenerative medicine	228:301	Low-fouling hydrogels with tunable degradation rates and biochemical environments have the potential to improve adoptive cell therapies for cancer immunotherapy and regenerative medicine.
31226699	0	27	theme	low-fouling	23:33	arg1	acid					61:64	low-fouling carboxybetaine-hyaluronic acid	23:64	low-fouling carboxybetaine-hyaluronic acid	23:64	Tunable degradation of low-fouling carboxybetaine-hyaluronic acid hydrogels for applications in cell encapsulation.
31226699	7	28	from	applications	1253:1264	arg1	therapies					1283:1291	cell adoptive therapies	1269:1291	cell adoptive therapies	1269:1291	Low-fouling pCBM-HA hydrogels provide a vehicle with tunable degradation rates and biochemical environments for encapsulation applications in cell adoptive therapies.
31226699	2	29	theme	gelling	338:344	arg1	hydrogels					346:354	in situ gelling hydrogels	330:354	in situ gelling hydrogels from low-fouling poly(carboxybetaine-co-maleimide) (pCBM) random copolymers and thiolated hyaluronic acid (HA-SH)	330:468	To this end, we developed in situ gelling hydrogels from low-fouling poly(carboxybetaine-co-maleimide) (pCBM) random copolymers and thiolated hyaluronic acid (HA-SH).
31226699	1	30	theme	cell	237:240	arg1	therapies					242:250	adoptive cell therapies	228:250	adoptive cell therapies for cancer immunotherapy and regenerative medicine	228:301	Low-fouling hydrogels with tunable degradation rates and biochemical environments have the potential to improve adoptive cell therapies for cancer immunotherapy and regenerative medicine.
31226699	3	31	theme	mol	588:590	arg1	%					591:591	4, 11, and 16 maleimide mol%	564:591	4, 11, and 16 maleimide mol%	564:591	pCBM-HA hydrogel enzymatic degradation rates were tuned 5 fold by altering pCBM composition (4, 11, and 16 maleimide mol%) and 2.3 fold by HA-SH concentration (1-2 wt%).
31226699	3	31	theme	mol	588:590	arg1	composition					551:561	pCBM composition	546:561	pCBM composition (4, 11, and 16 maleimide mol%)	546:592	pCBM-HA hydrogel enzymatic degradation rates were tuned 5 fold by altering pCBM composition (4, 11, and 16 maleimide mol%) and 2.3 fold by HA-SH concentration (1-2 wt%).
31226699	5	32	dep	adhesion	873:880	arg1	decrease					887:894	39% decrease	883:894	39% decrease in circularity	883:909	To control pCBM-HA bioactivity, the cell adhesive peptide CGRGDS was immobilized on pCBM to promote fibroblast adhesion (39% decrease in circularity), which increased metabolic activity by ∼50%.
31226699	3	33	theme	wt	635:636	arg1	%					637:637	1-2 wt%	631:637	1-2 wt%	631:637	pCBM-HA hydrogel enzymatic degradation rates were tuned 5 fold by altering pCBM composition (4, 11, and 16 maleimide mol%) and 2.3 fold by HA-SH concentration (1-2 wt%).
31226699	3	33	theme	wt	635:636	arg1	concentration					616:628	HA-SH concentration	610:628	HA-SH concentration (1-2 wt%)	610:638	pCBM-HA hydrogel enzymatic degradation rates were tuned 5 fold by altering pCBM composition (4, 11, and 16 maleimide mol%) and 2.3 fold by HA-SH concentration (1-2 wt%).
31226699	4	34	theme	∼20	715:717	arg1	cm-2					722:725	adsorbed ∼20 μg cm-2	706:725	BSA; adsorbed ∼20 μg cm-2	701:725	pCBM-HA gels were low-fouling towards bovine serum albumin (BSA; adsorbed ∼20 μg cm-2) and resisted fibroblast adhesion.
31226699	5	35	theme	cell	798:801	arg1	CGRGDS					820:825	the cell adhesive peptide CGRGDS	794:825	the cell adhesive peptide CGRGDS	794:825	To control pCBM-HA bioactivity, the cell adhesive peptide CGRGDS was immobilized on pCBM to promote fibroblast adhesion (39% decrease in circularity), which increased metabolic activity by ∼50%.
31226699	0	36	theme	acid	61:64	arg1	degradation					8:18	Tunable degradation	0:18	Tunable degradation of low-fouling carboxybetaine-hyaluronic acid	0:64	Tunable degradation of low-fouling carboxybetaine-hyaluronic acid hydrogels for applications in cell encapsulation.
31226699	4	37	theme	serum	686:690	arg1	albumin					692:698	bovine serum albumin	679:698	bovine serum albumin (BSA; adsorbed ∼20 μg cm-2)	679:726	pCBM-HA gels were low-fouling towards bovine serum albumin (BSA; adsorbed ∼20 μg cm-2) and resisted fibroblast adhesion.
31226699	4	37	theme	serum	686:690	arg1	BSA					701:703	BSA	701:703	BSA; adsorbed ∼20 μg cm-2	701:725	pCBM-HA gels were low-fouling towards bovine serum albumin (BSA; adsorbed ∼20 μg cm-2) and resisted fibroblast adhesion.
31226699	5	38	theme	adhesive	803:810	arg1	CGRGDS					820:825	the cell adhesive peptide CGRGDS	794:825	the cell adhesive peptide CGRGDS	794:825	To control pCBM-HA bioactivity, the cell adhesive peptide CGRGDS was immobilized on pCBM to promote fibroblast adhesion (39% decrease in circularity), which increased metabolic activity by ∼50%.
31226699	2	39	theme	random	414:419	arg1	copolymers					421:430	low-fouling poly(carboxybetaine-co-maleimide) (pCBM) random copolymers	361:430	low-fouling poly(carboxybetaine-co-maleimide) (pCBM) random copolymers	361:430	To this end, we developed in situ gelling hydrogels from low-fouling poly(carboxybetaine-co-maleimide) (pCBM) random copolymers and thiolated hyaluronic acid (HA-SH).
31226699	3	40	theme	enzymatic	488:496	arg1	rates					510:514	pCBM-HA hydrogel enzymatic degradation rates	471:514	pCBM-HA hydrogel enzymatic degradation rates	471:514	pCBM-HA hydrogel enzymatic degradation rates were tuned 5 fold by altering pCBM composition (4, 11, and 16 maleimide mol%) and 2.3 fold by HA-SH concentration (1-2 wt%).
31226699	0	41	theme	carboxybetaine-hyaluronic	35:59	arg1	acid					61:64	low-fouling carboxybetaine-hyaluronic acid	23:64	low-fouling carboxybetaine-hyaluronic acid	23:64	Tunable degradation of low-fouling carboxybetaine-hyaluronic acid hydrogels for applications in cell encapsulation.
31226699	2	42	from	copolymers	421:430	arg1	hydrogels					346:354	in situ gelling hydrogels	330:354	in situ gelling hydrogels from low-fouling poly(carboxybetaine-co-maleimide) (pCBM) random copolymers and thiolated hyaluronic acid (HA-SH)	330:468	To this end, we developed in situ gelling hydrogels from low-fouling poly(carboxybetaine-co-maleimide) (pCBM) random copolymers and thiolated hyaluronic acid (HA-SH).
31226699	1	43	theme	cancer	256:261	arg1	immunotherapy					263:275	cancer immunotherapy	256:275	cancer immunotherapy	256:275	Low-fouling hydrogels with tunable degradation rates and biochemical environments have the potential to improve adoptive cell therapies for cancer immunotherapy and regenerative medicine.
31226699	0	44	from	applications	80:91	arg1	encapsulation					101:113	cell encapsulation	96:113	cell encapsulation	96:113	Tunable degradation of low-fouling carboxybetaine-hyaluronic acid hydrogels for applications in cell encapsulation.
31226699	1	45	theme	tunable	143:149	arg1	rates					163:167	tunable degradation rates	143:167	tunable degradation rates	143:167	Low-fouling hydrogels with tunable degradation rates and biochemical environments have the potential to improve adoptive cell therapies for cancer immunotherapy and regenerative medicine.
31226699	7	46	theme	encapsulation	1239:1251	arg1	applications					1253:1264	encapsulation applications	1239:1264	encapsulation applications in cell adoptive therapies	1239:1291	Low-fouling pCBM-HA hydrogels provide a vehicle with tunable degradation rates and biochemical environments for encapsulation applications in cell adoptive therapies.
31226699	1	47	theme	degradation	151:161	arg1	rates					163:167	tunable degradation rates	143:167	tunable degradation rates	143:167	Low-fouling hydrogels with tunable degradation rates and biochemical environments have the potential to improve adoptive cell therapies for cancer immunotherapy and regenerative medicine.
31226699	3	48	theme	pCBM-HA	471:477	arg1	rates					510:514	pCBM-HA hydrogel enzymatic degradation rates	471:514	pCBM-HA hydrogel enzymatic degradation rates	471:514	pCBM-HA hydrogel enzymatic degradation rates were tuned 5 fold by altering pCBM composition (4, 11, and 16 maleimide mol%) and 2.3 fold by HA-SH concentration (1-2 wt%).
31226699	4	49	theme	pCBM-HA	641:647	arg1	gels					649:652	pCBM-HA gels	641:652	pCBM-HA gels	641:652	pCBM-HA gels were low-fouling towards bovine serum albumin (BSA; adsorbed ∼20 μg cm-2) and resisted fibroblast adhesion.
31226699	3	50	theme	HA-SH	610:614	arg1	%					637:637	1-2 wt%	631:637	1-2 wt%	631:637	pCBM-HA hydrogel enzymatic degradation rates were tuned 5 fold by altering pCBM composition (4, 11, and 16 maleimide mol%) and 2.3 fold by HA-SH concentration (1-2 wt%).
31226699	3	50	theme	HA-SH	610:614	arg1	concentration					616:628	HA-SH concentration	610:628	HA-SH concentration (1-2 wt%)	610:638	pCBM-HA hydrogel enzymatic degradation rates were tuned 5 fold by altering pCBM composition (4, 11, and 16 maleimide mol%) and 2.3 fold by HA-SH concentration (1-2 wt%).
31226699	4	51	dep	BSA	701:703	arg1	cm-2					722:725	adsorbed ∼20 μg cm-2	706:725	BSA; adsorbed ∼20 μg cm-2	701:725	pCBM-HA gels were low-fouling towards bovine serum albumin (BSA; adsorbed ∼20 μg cm-2) and resisted fibroblast adhesion.
31226699	2	52	theme	poly	373:376	arg1	copolymers					421:430	low-fouling poly(carboxybetaine-co-maleimide) (pCBM) random copolymers	361:430	low-fouling poly(carboxybetaine-co-maleimide) (pCBM) random copolymers	361:430	To this end, we developed in situ gelling hydrogels from low-fouling poly(carboxybetaine-co-maleimide) (pCBM) random copolymers and thiolated hyaluronic acid (HA-SH).
31226699	4	53	theme	μg	719:720	arg1	cm-2					722:725	adsorbed ∼20 μg cm-2	706:725	BSA; adsorbed ∼20 μg cm-2	701:725	pCBM-HA gels were low-fouling towards bovine serum albumin (BSA; adsorbed ∼20 μg cm-2) and resisted fibroblast adhesion.
31226699	5	54	theme	metabolic	929:937	arg1	activity					939:946	metabolic activity	929:946	metabolic activity	929:946	To control pCBM-HA bioactivity, the cell adhesive peptide CGRGDS was immobilized on pCBM to promote fibroblast adhesion (39% decrease in circularity), which increased metabolic activity by ∼50%.
31226699	4	55	theme	fibroblast	741:750	arg1	adhesion					752:759	resisted fibroblast adhesion	732:759	resisted fibroblast adhesion	732:759	pCBM-HA gels were low-fouling towards bovine serum albumin (BSA; adsorbed ∼20 μg cm-2) and resisted fibroblast adhesion.
31226699	2	56	from	acid	457:460	arg1	hydrogels					346:354	in situ gelling hydrogels	330:354	in situ gelling hydrogels from low-fouling poly(carboxybetaine-co-maleimide) (pCBM) random copolymers and thiolated hyaluronic acid (HA-SH)	330:468	To this end, we developed in situ gelling hydrogels from low-fouling poly(carboxybetaine-co-maleimide) (pCBM) random copolymers and thiolated hyaluronic acid (HA-SH).
31226699	2	57	theme	low-fouling	361:371	arg1	carboxybetaine-co-maleimide					378:404	carboxybetaine-co-maleimide	378:404	carboxybetaine-co-maleimide	378:404	To this end, we developed in situ gelling hydrogels from low-fouling poly(carboxybetaine-co-maleimide) (pCBM) random copolymers and thiolated hyaluronic acid (HA-SH).
31226699	2	57	theme	low-fouling	361:371	arg1	pCBM					408:411	pCBM	408:411	pCBM	408:411	To this end, we developed in situ gelling hydrogels from low-fouling poly(carboxybetaine-co-maleimide) (pCBM) random copolymers and thiolated hyaluronic acid (HA-SH).
31226699	2	57	theme	low-fouling	361:371	arg1	poly					373:376	low-fouling poly	361:376	low-fouling poly(carboxybetaine-co-maleimide) (pCBM) random copolymers	361:430	To this end, we developed in situ gelling hydrogels from low-fouling poly(carboxybetaine-co-maleimide) (pCBM) random copolymers and thiolated hyaluronic acid (HA-SH).
31226699	5	58	from	decrease	887:894	arg1	circularity					899:909	circularity	899:909	circularity	899:909	To control pCBM-HA bioactivity, the cell adhesive peptide CGRGDS was immobilized on pCBM to promote fibroblast adhesion (39% decrease in circularity), which increased metabolic activity by ∼50%.
31226699	5	59	theme	39	883:884	arg1	%					885:885	%	885:885	%	885:885	To control pCBM-HA bioactivity, the cell adhesive peptide CGRGDS was immobilized on pCBM to promote fibroblast adhesion (39% decrease in circularity), which increased metabolic activity by ∼50%.
31226699	2	60	theme	hyaluronic	446:455	arg1	acid					457:460	thiolated hyaluronic acid	436:460	thiolated hyaluronic acid (HA-SH)	436:468	To this end, we developed in situ gelling hydrogels from low-fouling poly(carboxybetaine-co-maleimide) (pCBM) random copolymers and thiolated hyaluronic acid (HA-SH).
31226699	2	60	theme	hyaluronic	446:455	arg1	HA-SH					463:467	HA-SH	463:467	HA-SH	463:467	To this end, we developed in situ gelling hydrogels from low-fouling poly(carboxybetaine-co-maleimide) (pCBM) random copolymers and thiolated hyaluronic acid (HA-SH).
31226699	6	61	theme	metabolic	999:1007	arg1	activity					1009:1016	the metabolic activity	995:1016	the metabolic activity of encapsulated T cells	995:1040	pCBM-HA modified with CGRGDS enhanced the metabolic activity of encapsulated T cells by ∼21% compared to gels without HA, indicating their potential for immunotherapies.
31226699	1	62	theme	biochemical	173:183	arg1	environments					185:196	biochemical environments	173:196	biochemical environments	173:196	Low-fouling hydrogels with tunable degradation rates and biochemical environments have the potential to improve adoptive cell therapies for cancer immunotherapy and regenerative medicine.
31226699	6	63	theme	encapsulated	1021:1032	arg1	cells					1036:1040	encapsulated T cells	1021:1040	encapsulated T cells	1021:1040	pCBM-HA modified with CGRGDS enhanced the metabolic activity of encapsulated T cells by ∼21% compared to gels without HA, indicating their potential for immunotherapies.
31226699	1	64	theme	regenerative	281:292	arg1	medicine					294:301	regenerative medicine	281:301	regenerative medicine	281:301	Low-fouling hydrogels with tunable degradation rates and biochemical environments have the potential to improve adoptive cell therapies for cancer immunotherapy and regenerative medicine.
31226699	7	65	theme	tunable	1180:1186	arg1	rates					1200:1204	tunable degradation rates	1180:1204	tunable degradation rates	1180:1204	Low-fouling pCBM-HA hydrogels provide a vehicle with tunable degradation rates and biochemical environments for encapsulation applications in cell adoptive therapies.
31226699	7	66	theme	biochemical	1210:1220	arg1	environments					1222:1233	biochemical environments	1210:1233	biochemical environments	1210:1233	Low-fouling pCBM-HA hydrogels provide a vehicle with tunable degradation rates and biochemical environments for encapsulation applications in cell adoptive therapies.
31226699	7	67	theme	degradation	1188:1198	arg1	rates					1200:1204	tunable degradation rates	1180:1204	tunable degradation rates	1180:1204	Low-fouling pCBM-HA hydrogels provide a vehicle with tunable degradation rates and biochemical environments for encapsulation applications in cell adoptive therapies.
30597238	6	0	theme	release	1100:1106	arg1	rate					1108:1111	the release rate	1096:1111	the release rate of ciprofloxacin drugs	1096:1134	The study of the release rate of ciprofloxacin drugs is carried out by measuring water retention and swelling properties of nanocomposite hydrogels.
30597238	2	1	theme	X-ray	576:580	arg1	XPS					610:612	XPS	610:612	XPS	610:612	The structure, composition, morphology behaviour of the nanocomposite hydrogels are explored using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction study (XRD), X-ray photoelectron spectroscopy (XPS); field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and atomic force microscopy (AFM).
30597238	2	1	theme	X-ray	576:580	arg1	spectroscopy					596:607	X-ray photoelectron spectroscopy	576:607	X-ray photoelectron spectroscopy (XPS)	576:613	The structure, composition, morphology behaviour of the nanocomposite hydrogels are explored using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction study (XRD), X-ray photoelectron spectroscopy (XPS); field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and atomic force microscopy (AFM).
30597238	4	2	theme	prepared	854:861	arg1	hydrogels					877:885	prepared nanocomposite hydrogels	854:885	prepared nanocomposite hydrogels	854:885	The gelling actions of prepared nanocomposite hydrogels are determined by the rheological study.
30597238	2	3	theme	emission	622:629	arg1	microscopy					649:658	field emission scanning electron microscopy	616:658	field emission scanning electron microscopy (FESEM)	616:666	The structure, composition, morphology behaviour of the nanocomposite hydrogels are explored using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction study (XRD), X-ray photoelectron spectroscopy (XPS); field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and atomic force microscopy (AFM).
30597238	2	3	theme	emission	622:629	arg1	FESEM					661:665	FESEM	661:665	FESEM	661:665	The structure, composition, morphology behaviour of the nanocomposite hydrogels are explored using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction study (XRD), X-ray photoelectron spectroscopy (XPS); field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and atomic force microscopy (AFM).
30597238	1	4	theme	gold	177:180	arg1	hydrogel					249:256	the porous nano gold embedded cellulose grafted polyacrylamide (PAM/C/Au) nanocomposite hydrogel	161:256	the porous nano gold embedded cellulose grafted polyacrylamide (PAM/C/Au) nanocomposite hydrogel which has been prepared by in situ polymerization process with an objective of application for the in vitro release of ciprofloxacin drugs	161:395	The present work involves the designing of the porous nano gold embedded cellulose grafted polyacrylamide (PAM/C/Au) nanocomposite hydrogel which has been prepared by in situ polymerization process with an objective of application for the in vitro release of ciprofloxacin drugs.
30597238	1	5	with	process	308:314	arg1	objective					324:332	an objective	321:332	an objective of application for the in vitro release of ciprofloxacin drugs	321:395	The present work involves the designing of the porous nano gold embedded cellulose grafted polyacrylamide (PAM/C/Au) nanocomposite hydrogel which has been prepared by in situ polymerization process with an objective of application for the in vitro release of ciprofloxacin drugs.
30597238	6	6	theme	water	1164:1168	arg1	retention					1170:1178	water retention	1164:1178	water retention	1164:1178	The study of the release rate of ciprofloxacin drugs is carried out by measuring water retention and swelling properties of nanocomposite hydrogels.
30597238	1	7	theme	cellulose	191:199	arg1	hydrogel					249:256	the porous nano gold embedded cellulose grafted polyacrylamide (PAM/C/Au) nanocomposite hydrogel	161:256	the porous nano gold embedded cellulose grafted polyacrylamide (PAM/C/Au) nanocomposite hydrogel which has been prepared by in situ polymerization process with an objective of application for the in vitro release of ciprofloxacin drugs	161:395	The present work involves the designing of the porous nano gold embedded cellulose grafted polyacrylamide (PAM/C/Au) nanocomposite hydrogel which has been prepared by in situ polymerization process with an objective of application for the in vitro release of ciprofloxacin drugs.
30597238	1	8	theme	drugs	391:395	arg1	release					366:372	the in vitro release	353:372	the in vitro release of ciprofloxacin drugs	353:395	The present work involves the designing of the porous nano gold embedded cellulose grafted polyacrylamide (PAM/C/Au) nanocomposite hydrogel which has been prepared by in situ polymerization process with an objective of application for the in vitro release of ciprofloxacin drugs.
30597238	1	9	theme	polyacrylamide	209:222	arg1	hydrogel					249:256	the porous nano gold embedded cellulose grafted polyacrylamide (PAM/C/Au) nanocomposite hydrogel	161:256	the porous nano gold embedded cellulose grafted polyacrylamide (PAM/C/Au) nanocomposite hydrogel which has been prepared by in situ polymerization process with an objective of application for the in vitro release of ciprofloxacin drugs	161:395	The present work involves the designing of the porous nano gold embedded cellulose grafted polyacrylamide (PAM/C/Au) nanocomposite hydrogel which has been prepared by in situ polymerization process with an objective of application for the in vitro release of ciprofloxacin drugs.
30597238	2	10	theme	diffraction	551:561	arg1	study					563:567	X-ray diffraction study	545:567	X-ray diffraction study (XRD)	545:573	The structure, composition, morphology behaviour of the nanocomposite hydrogels are explored using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction study (XRD), X-ray photoelectron spectroscopy (XPS); field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and atomic force microscopy (AFM).
30597238	2	10	theme	diffraction	551:561	arg1	XRD					570:572	XRD	570:572	XRD	570:572	The structure, composition, morphology behaviour of the nanocomposite hydrogels are explored using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction study (XRD), X-ray photoelectron spectroscopy (XPS); field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and atomic force microscopy (AFM).
30597238	8	11	theme	in	1454:1455	arg1	release					1463:1469	in vitro release	1454:1469	in vitro release of the ciprofloxacin drugs	1454:1496	The PAM/C/Au nanocomposite hydrogels with improved thermal and rheological properties are suitable proposed as a good carrier towards in vitro release of the ciprofloxacin drugs.
30597238	3	12	theme	thermal	747:753	arg1	stability					755:763	The thermal stability	743:763	The thermal stability of the as synthesized nanocomposite hydrogels	743:809	The thermal stability of the as synthesized nanocomposite hydrogels are studied by TGA.
30597238	1	13	theme	PAM/C/Au	225:232	arg1	hydrogel					249:256	the porous nano gold embedded cellulose grafted polyacrylamide (PAM/C/Au) nanocomposite hydrogel	161:256	the porous nano gold embedded cellulose grafted polyacrylamide (PAM/C/Au) nanocomposite hydrogel which has been prepared by in situ polymerization process with an objective of application for the in vitro release of ciprofloxacin drugs	161:395	The present work involves the designing of the porous nano gold embedded cellulose grafted polyacrylamide (PAM/C/Au) nanocomposite hydrogel which has been prepared by in situ polymerization process with an objective of application for the in vitro release of ciprofloxacin drugs.
30597238	4	14	theme	hydrogels	877:885	arg1	actions					843:849	The gelling actions	831:849	The gelling actions of prepared nanocomposite hydrogels	831:885	The gelling actions of prepared nanocomposite hydrogels are determined by the rheological study.
30597238	5	15	theme	nanocomposite	1041:1053	arg1	hydrogels					1055:1063	nanocomposite hydrogels	1041:1063	nanocomposite hydrogels	1041:1063	The investigations of cytotoxicity tests and antibacterial behaviour along with negative and positive actions of nanocomposite hydrogels are investigated.
30597238	2	16	theme	electron	682:689	arg1	TEM					703:705	TEM	703:705	TEM	703:705	The structure, composition, morphology behaviour of the nanocomposite hydrogels are explored using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction study (XRD), X-ray photoelectron spectroscopy (XPS); field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and atomic force microscopy (AFM).
30597238	2	16	theme	electron	682:689	arg1	microscopy					691:700	transmission electron microscopy	669:700	transmission electron microscopy (TEM)	669:706	The structure, composition, morphology behaviour of the nanocomposite hydrogels are explored using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction study (XRD), X-ray photoelectron spectroscopy (XPS); field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and atomic force microscopy (AFM).
30597238	8	17	theme	drugs	1492:1496	arg1	release					1463:1469	in vitro release	1454:1469	in vitro release of the ciprofloxacin drugs	1454:1496	The PAM/C/Au nanocomposite hydrogels with improved thermal and rheological properties are suitable proposed as a good carrier towards in vitro release of the ciprofloxacin drugs.
30597238	6	18	theme	nanocomposite	1207:1219	arg1	hydrogels					1221:1229	nanocomposite hydrogels	1207:1229	nanocomposite hydrogels	1207:1229	The study of the release rate of ciprofloxacin drugs is carried out by measuring water retention and swelling properties of nanocomposite hydrogels.
30597238	2	19	theme	atomic	712:717	arg1	AFM					737:739	AFM	737:739	AFM	737:739	The structure, composition, morphology behaviour of the nanocomposite hydrogels are explored using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction study (XRD), X-ray photoelectron spectroscopy (XPS); field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and atomic force microscopy (AFM).
30597238	2	19	theme	atomic	712:717	arg1	microscopy					725:734	atomic force microscopy	712:734	atomic force microscopy (AFM)	712:740	The structure, composition, morphology behaviour of the nanocomposite hydrogels are explored using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction study (XRD), X-ray photoelectron spectroscopy (XPS); field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and atomic force microscopy (AFM).
30597238	8	20	with	hydrogels	1347:1355	arg1	thermal					1371:1377	thermal	1371:1377	thermal	1371:1377	The PAM/C/Au nanocomposite hydrogels with improved thermal and rheological properties are suitable proposed as a good carrier towards in vitro release of the ciprofloxacin drugs.
30597238	8	20	with	hydrogels	1347:1355	arg1	properties					1395:1404	improved thermal and rheological properties	1362:1404	properties	1395:1404	The PAM/C/Au nanocomposite hydrogels with improved thermal and rheological properties are suitable proposed as a good carrier towards in vitro release of the ciprofloxacin drugs.
30597238	7	21	theme	release	1245:1251	arg1	%					1310:1310	96.6%	1306:1310	96.6%	1306:1310	The in vitro release rate of ciprofloxacin antibiotic drug is found to be 96.6% in 5 h.
30597238	7	21	theme	release	1245:1251	arg1	rate					1253:1256	The in vitro release rate	1232:1256	The in vitro release rate of ciprofloxacin antibiotic drug	1232:1289	The in vitro release rate of ciprofloxacin antibiotic drug is found to be 96.6% in 5 h.
30597238	1	22	theme	application	337:347	arg1	objective					324:332	an objective	321:332	an objective of application for the in vitro release of ciprofloxacin drugs	321:395	The present work involves the designing of the porous nano gold embedded cellulose grafted polyacrylamide (PAM/C/Au) nanocomposite hydrogel which has been prepared by in situ polymerization process with an objective of application for the in vitro release of ciprofloxacin drugs.
30597238	8	23	theme	PAM/C/Au	1324:1331	arg1	proposed					1419:1426	proposed	1419:1426	proposed as a good carrier towards in vitro release of the ciprofloxacin drugs	1419:1496	The PAM/C/Au nanocomposite hydrogels with improved thermal and rheological properties are suitable proposed as a good carrier towards in vitro release of the ciprofloxacin drugs.
30597238	8	23	theme	PAM/C/Au	1324:1331	arg1	hydrogels					1347:1355	The PAM/C/Au nanocomposite hydrogels	1320:1355	The PAM/C/Au nanocomposite hydrogels with improved thermal and rheological properties	1320:1404	The PAM/C/Au nanocomposite hydrogels with improved thermal and rheological properties are suitable proposed as a good carrier towards in vitro release of the ciprofloxacin drugs.
30597238	8	23	theme	PAM/C/Au	1324:1331	arg1	suitable					1410:1417	suitable	1410:1417	suitable	1410:1417	The PAM/C/Au nanocomposite hydrogels with improved thermal and rheological properties are suitable proposed as a good carrier towards in vitro release of the ciprofloxacin drugs.
30597238	0	24	theme	polyacrylamide	71:84	arg1	hydrogels					107:115	polyacrylamide hybrid nanocomposite hydrogels	71:115	polyacrylamide hybrid nanocomposite hydrogels	71:115	Release of ciprofloxacin drugs by nano gold embedded cellulose grafted polyacrylamide hybrid nanocomposite hydrogels.
30597238	1	25	theme	porous	165:170	arg1	hydrogel					249:256	the porous nano gold embedded cellulose grafted polyacrylamide (PAM/C/Au) nanocomposite hydrogel	161:256	the porous nano gold embedded cellulose grafted polyacrylamide (PAM/C/Au) nanocomposite hydrogel which has been prepared by in situ polymerization process with an objective of application for the in vitro release of ciprofloxacin drugs	161:395	The present work involves the designing of the porous nano gold embedded cellulose grafted polyacrylamide (PAM/C/Au) nanocomposite hydrogel which has been prepared by in situ polymerization process with an objective of application for the in vitro release of ciprofloxacin drugs.
30597238	3	26	theme	nanocomposite	787:799	arg1	hydrogels					801:809	the as synthesized nanocomposite hydrogels	768:809	the as synthesized nanocomposite hydrogels	768:809	The thermal stability of the as synthesized nanocomposite hydrogels are studied by TGA.
30597238	0	27	theme	nanocomposite	93:105	arg1	hydrogels					107:115	polyacrylamide hybrid nanocomposite hydrogels	71:115	polyacrylamide hybrid nanocomposite hydrogels	71:115	Release of ciprofloxacin drugs by nano gold embedded cellulose grafted polyacrylamide hybrid nanocomposite hydrogels.
30597238	5	28	theme	tests	963:967	arg1	investigations					932:945	The investigations	928:945	The investigations of cytotoxicity tests and antibacterial behaviour along with negative and positive actions of nanocomposite hydrogels	928:1063	The investigations of cytotoxicity tests and antibacterial behaviour along with negative and positive actions of nanocomposite hydrogels are investigated.
30597238	2	29	theme	scanning	631:638	arg1	microscopy					649:658	field emission scanning electron microscopy	616:658	field emission scanning electron microscopy (FESEM)	616:666	The structure, composition, morphology behaviour of the nanocomposite hydrogels are explored using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction study (XRD), X-ray photoelectron spectroscopy (XPS); field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and atomic force microscopy (AFM).
30597238	2	29	theme	scanning	631:638	arg1	FESEM					661:665	FESEM	661:665	FESEM	661:665	The structure, composition, morphology behaviour of the nanocomposite hydrogels are explored using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction study (XRD), X-ray photoelectron spectroscopy (XPS); field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and atomic force microscopy (AFM).
30597238	5	30	theme	antibacterial	973:985	arg1	behaviour					987:995	antibacterial behaviour	973:995	antibacterial behaviour	973:995	The investigations of cytotoxicity tests and antibacterial behaviour along with negative and positive actions of nanocomposite hydrogels are investigated.
30597238	2	31	theme	nanocomposite	454:466	arg1	hydrogels					468:476	the nanocomposite hydrogels	450:476	the nanocomposite hydrogels	450:476	The structure, composition, morphology behaviour of the nanocomposite hydrogels are explored using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction study (XRD), X-ray photoelectron spectroscopy (XPS); field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and atomic force microscopy (AFM).
30597238	1	32	theme	in	285:286	arg1	process					308:314	in situ polymerization process	285:314	in situ polymerization process with an objective of application for the in vitro release of ciprofloxacin drugs	285:395	The present work involves the designing of the porous nano gold embedded cellulose grafted polyacrylamide (PAM/C/Au) nanocomposite hydrogel which has been prepared by in situ polymerization process with an objective of application for the in vitro release of ciprofloxacin drugs.
30597238	1	33	dep	in	357:358	arg1	vitro					360:364	vitro	360:364	vitro	360:364	The present work involves the designing of the porous nano gold embedded cellulose grafted polyacrylamide (PAM/C/Au) nanocomposite hydrogel which has been prepared by in situ polymerization process with an objective of application for the in vitro release of ciprofloxacin drugs.
30597238	2	34	theme	morphology	426:435	arg1	behaviour					437:445	morphology behaviour	426:445	morphology behaviour	426:445	The structure, composition, morphology behaviour of the nanocomposite hydrogels are explored using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction study (XRD), X-ray photoelectron spectroscopy (XPS); field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and atomic force microscopy (AFM).
30597238	2	34	theme	morphology	426:435	arg1	structure					402:410	The structure	398:410	The structure	398:410	The structure, composition, morphology behaviour of the nanocomposite hydrogels are explored using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction study (XRD), X-ray photoelectron spectroscopy (XPS); field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and atomic force microscopy (AFM).
30597238	0	35	theme	ciprofloxacin	11:23	arg1	drugs					25:29	ciprofloxacin drugs	11:29	ciprofloxacin drugs	11:29	Release of ciprofloxacin drugs by nano gold embedded cellulose grafted polyacrylamide hybrid nanocomposite hydrogels.
30597238	1	36	theme	polymerization	293:306	arg1	process					308:314	in situ polymerization process	285:314	in situ polymerization process with an objective of application for the in vitro release of ciprofloxacin drugs	285:395	The present work involves the designing of the porous nano gold embedded cellulose grafted polyacrylamide (PAM/C/Au) nanocomposite hydrogel which has been prepared by in situ polymerization process with an objective of application for the in vitro release of ciprofloxacin drugs.
30597238	5	37	theme	positive	1021:1028	arg1	actions					1030:1036	negative and positive actions	1008:1036	negative and positive actions of nanocomposite hydrogels	1008:1063	The investigations of cytotoxicity tests and antibacterial behaviour along with negative and positive actions of nanocomposite hydrogels are investigated.
30597238	2	38	theme	X-ray	545:549	arg1	study					563:567	X-ray diffraction study	545:567	X-ray diffraction study (XRD)	545:573	The structure, composition, morphology behaviour of the nanocomposite hydrogels are explored using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction study (XRD), X-ray photoelectron spectroscopy (XPS); field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and atomic force microscopy (AFM).
30597238	2	38	theme	X-ray	545:549	arg1	XRD					570:572	XRD	570:572	XRD	570:572	The structure, composition, morphology behaviour of the nanocomposite hydrogels are explored using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction study (XRD), X-ray photoelectron spectroscopy (XPS); field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and atomic force microscopy (AFM).
30597238	0	39	theme	gold	39:42	arg1	cellulose					53:61	nano gold embedded cellulose	34:61	nano gold embedded cellulose	34:61	Release of ciprofloxacin drugs by nano gold embedded cellulose grafted polyacrylamide hybrid nanocomposite hydrogels.
30597238	2	40	dep	Fourier	497:503	arg1	transform					505:513	transform	505:513	transform infrared (FTIR) spectroscopy, X-ray diffraction study (XRD), X-ray photoelectron spectroscopy (XPS); field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and atomic force microscopy (AFM)	505:740	The structure, composition, morphology behaviour of the nanocomposite hydrogels are explored using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction study (XRD), X-ray photoelectron spectroscopy (XPS); field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and atomic force microscopy (AFM).
30597238	7	41	from	%	1310:1310	arg1	5 h					1315:1317	5 h	1315:1317	5 h	1315:1317	The in vitro release rate of ciprofloxacin antibiotic drug is found to be 96.6% in 5 h.
30597238	8	42	theme	good	1433:1436	arg1	carrier					1438:1444	a good carrier	1431:1444	a good carrier towards in vitro release of the ciprofloxacin drugs	1431:1496	The PAM/C/Au nanocomposite hydrogels with improved thermal and rheological properties are suitable proposed as a good carrier towards in vitro release of the ciprofloxacin drugs.
30597238	2	43	dep	infrared	515:522	arg1	FTIR					525:528	FTIR	525:528	FTIR	525:528	The structure, composition, morphology behaviour of the nanocomposite hydrogels are explored using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction study (XRD), X-ray photoelectron spectroscopy (XPS); field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and atomic force microscopy (AFM).
30597238	6	44	theme	ciprofloxacin	1116:1128	arg1	drugs					1130:1134	ciprofloxacin drugs	1116:1134	ciprofloxacin drugs	1116:1134	The study of the release rate of ciprofloxacin drugs is carried out by measuring water retention and swelling properties of nanocomposite hydrogels.
30597238	1	45	theme	hydrogel	249:256	arg1	designing					148:156	designing	148:156	designing	148:156	The present work involves the designing of the porous nano gold embedded cellulose grafted polyacrylamide (PAM/C/Au) nanocomposite hydrogel which has been prepared by in situ polymerization process with an objective of application for the in vitro release of ciprofloxacin drugs.
30597238	5	46	theme	negative	1008:1015	arg1	actions					1030:1036	negative and positive actions	1008:1036	negative and positive actions of nanocomposite hydrogels	1008:1063	The investigations of cytotoxicity tests and antibacterial behaviour along with negative and positive actions of nanocomposite hydrogels are investigated.
30597238	7	47	theme	antibiotic	1275:1284	arg1	drug					1286:1289	ciprofloxacin antibiotic drug	1261:1289	ciprofloxacin antibiotic drug	1261:1289	The in vitro release rate of ciprofloxacin antibiotic drug is found to be 96.6% in 5 h.
30597238	4	48	theme	gelling	835:841	arg1	actions					843:849	The gelling actions	831:849	The gelling actions of prepared nanocomposite hydrogels	831:885	The gelling actions of prepared nanocomposite hydrogels are determined by the rheological study.
30597238	2	49	theme	photoelectron	582:594	arg1	XPS					610:612	XPS	610:612	XPS	610:612	The structure, composition, morphology behaviour of the nanocomposite hydrogels are explored using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction study (XRD), X-ray photoelectron spectroscopy (XPS); field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and atomic force microscopy (AFM).
30597238	2	49	theme	photoelectron	582:594	arg1	spectroscopy					596:607	X-ray photoelectron spectroscopy	576:607	X-ray photoelectron spectroscopy (XPS)	576:613	The structure, composition, morphology behaviour of the nanocomposite hydrogels are explored using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction study (XRD), X-ray photoelectron spectroscopy (XPS); field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and atomic force microscopy (AFM).
30597238	6	50	theme	swelling	1184:1191	arg1	properties					1193:1202	swelling properties	1184:1202	swelling properties of nanocomposite hydrogels	1184:1229	The study of the release rate of ciprofloxacin drugs is carried out by measuring water retention and swelling properties of nanocomposite hydrogels.
30597238	1	51	theme	nano	172:175	arg1	hydrogel					249:256	the porous nano gold embedded cellulose grafted polyacrylamide (PAM/C/Au) nanocomposite hydrogel	161:256	the porous nano gold embedded cellulose grafted polyacrylamide (PAM/C/Au) nanocomposite hydrogel which has been prepared by in situ polymerization process with an objective of application for the in vitro release of ciprofloxacin drugs	161:395	The present work involves the designing of the porous nano gold embedded cellulose grafted polyacrylamide (PAM/C/Au) nanocomposite hydrogel which has been prepared by in situ polymerization process with an objective of application for the in vitro release of ciprofloxacin drugs.
30597238	2	52	theme	field	616:620	arg1	microscopy					649:658	field emission scanning electron microscopy	616:658	field emission scanning electron microscopy (FESEM)	616:666	The structure, composition, morphology behaviour of the nanocomposite hydrogels are explored using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction study (XRD), X-ray photoelectron spectroscopy (XPS); field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and atomic force microscopy (AFM).
30597238	2	52	theme	field	616:620	arg1	FESEM					661:665	FESEM	661:665	FESEM	661:665	The structure, composition, morphology behaviour of the nanocomposite hydrogels are explored using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction study (XRD), X-ray photoelectron spectroscopy (XPS); field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and atomic force microscopy (AFM).
30597238	7	53	theme	in	1236:1237	arg1	%					1310:1310	96.6%	1306:1310	96.6%	1306:1310	The in vitro release rate of ciprofloxacin antibiotic drug is found to be 96.6% in 5 h.
30597238	7	53	theme	in	1236:1237	arg1	rate					1253:1256	The in vitro release rate	1232:1256	The in vitro release rate of ciprofloxacin antibiotic drug	1232:1289	The in vitro release rate of ciprofloxacin antibiotic drug is found to be 96.6% in 5 h.
30597238	1	54	theme	embedded	182:189	arg1	hydrogel					249:256	the porous nano gold embedded cellulose grafted polyacrylamide (PAM/C/Au) nanocomposite hydrogel	161:256	the porous nano gold embedded cellulose grafted polyacrylamide (PAM/C/Au) nanocomposite hydrogel which has been prepared by in situ polymerization process with an objective of application for the in vitro release of ciprofloxacin drugs	161:395	The present work involves the designing of the porous nano gold embedded cellulose grafted polyacrylamide (PAM/C/Au) nanocomposite hydrogel which has been prepared by in situ polymerization process with an objective of application for the in vitro release of ciprofloxacin drugs.
30597238	1	55	theme	grafted	201:207	arg1	hydrogel					249:256	the porous nano gold embedded cellulose grafted polyacrylamide (PAM/C/Au) nanocomposite hydrogel	161:256	the porous nano gold embedded cellulose grafted polyacrylamide (PAM/C/Au) nanocomposite hydrogel which has been prepared by in situ polymerization process with an objective of application for the in vitro release of ciprofloxacin drugs	161:395	The present work involves the designing of the porous nano gold embedded cellulose grafted polyacrylamide (PAM/C/Au) nanocomposite hydrogel which has been prepared by in situ polymerization process with an objective of application for the in vitro release of ciprofloxacin drugs.
30597238	4	56	theme	rheological	909:919	arg1	study					921:925	the rheological study	905:925	the rheological study	905:925	The gelling actions of prepared nanocomposite hydrogels are determined by the rheological study.
30597238	1	57	theme	present	122:128	arg1	work					130:133	The present work	118:133	The present work	118:133	The present work involves the designing of the porous nano gold embedded cellulose grafted polyacrylamide (PAM/C/Au) nanocomposite hydrogel which has been prepared by in situ polymerization process with an objective of application for the in vitro release of ciprofloxacin drugs.
30597238	4	58	theme	nanocomposite	863:875	arg1	hydrogels					877:885	prepared nanocomposite hydrogels	854:885	prepared nanocomposite hydrogels	854:885	The gelling actions of prepared nanocomposite hydrogels are determined by the rheological study.
30597238	8	59	theme	ciprofloxacin	1478:1490	arg1	drugs					1492:1496	the ciprofloxacin drugs	1474:1496	the ciprofloxacin drugs	1474:1496	The PAM/C/Au nanocomposite hydrogels with improved thermal and rheological properties are suitable proposed as a good carrier towards in vitro release of the ciprofloxacin drugs.
30597238	5	60	theme	hydrogels	1055:1063	arg1	actions					1030:1036	negative and positive actions	1008:1036	negative and positive actions of nanocomposite hydrogels	1008:1063	The investigations of cytotoxicity tests and antibacterial behaviour along with negative and positive actions of nanocomposite hydrogels are investigated.
30597238	6	61	theme	hydrogels	1221:1229	arg1	retention					1170:1178	water retention	1164:1178	water retention	1164:1178	The study of the release rate of ciprofloxacin drugs is carried out by measuring water retention and swelling properties of nanocomposite hydrogels.
30597238	6	61	theme	hydrogels	1221:1229	arg1	properties					1193:1202	swelling properties	1184:1202	swelling properties of nanocomposite hydrogels	1184:1229	The study of the release rate of ciprofloxacin drugs is carried out by measuring water retention and swelling properties of nanocomposite hydrogels.
30597238	2	62	theme	force	719:723	arg1	AFM					737:739	AFM	737:739	AFM	737:739	The structure, composition, morphology behaviour of the nanocomposite hydrogels are explored using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction study (XRD), X-ray photoelectron spectroscopy (XPS); field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and atomic force microscopy (AFM).
30597238	2	62	theme	force	719:723	arg1	microscopy					725:734	atomic force microscopy	712:734	atomic force microscopy (AFM)	712:740	The structure, composition, morphology behaviour of the nanocomposite hydrogels are explored using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction study (XRD), X-ray photoelectron spectroscopy (XPS); field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and atomic force microscopy (AFM).
30597238	3	63	theme	synthesized	775:785	arg1	hydrogels					801:809	the as synthesized nanocomposite hydrogels	768:809	the as synthesized nanocomposite hydrogels	768:809	The thermal stability of the as synthesized nanocomposite hydrogels are studied by TGA.
30597238	2	64	theme	infrared	515:522	arg1	spectroscopy					531:542	infrared (FTIR) spectroscopy	515:542	infrared (FTIR) spectroscopy	515:542	The structure, composition, morphology behaviour of the nanocomposite hydrogels are explored using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction study (XRD), X-ray photoelectron spectroscopy (XPS); field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and atomic force microscopy (AFM).
30597238	0	65	theme	hybrid	86:91	arg1	hydrogels					107:115	polyacrylamide hybrid nanocomposite hydrogels	71:115	polyacrylamide hybrid nanocomposite hydrogels	71:115	Release of ciprofloxacin drugs by nano gold embedded cellulose grafted polyacrylamide hybrid nanocomposite hydrogels.
30597238	8	66	dep	suitable	1410:1417	arg1	proposed					1419:1426	proposed	1419:1426	proposed as a good carrier towards in vitro release of the ciprofloxacin drugs	1419:1496	The PAM/C/Au nanocomposite hydrogels with improved thermal and rheological properties are suitable proposed as a good carrier towards in vitro release of the ciprofloxacin drugs.
30597238	8	66	dep	suitable	1410:1417	arg1	hydrogels					1347:1355	The PAM/C/Au nanocomposite hydrogels	1320:1355	The PAM/C/Au nanocomposite hydrogels with improved thermal and rheological properties	1320:1404	The PAM/C/Au nanocomposite hydrogels with improved thermal and rheological properties are suitable proposed as a good carrier towards in vitro release of the ciprofloxacin drugs.
30597238	8	66	dep	suitable	1410:1417	arg1	suitable					1410:1417	suitable	1410:1417	suitable	1410:1417	The PAM/C/Au nanocomposite hydrogels with improved thermal and rheological properties are suitable proposed as a good carrier towards in vitro release of the ciprofloxacin drugs.
30597238	1	67	dep	in	285:286	arg1	situ					288:291	situ	288:291	situ	288:291	The present work involves the designing of the porous nano gold embedded cellulose grafted polyacrylamide (PAM/C/Au) nanocomposite hydrogel which has been prepared by in situ polymerization process with an objective of application for the in vitro release of ciprofloxacin drugs.
30597238	1	68	theme	in	357:358	arg1	release					366:372	the in vitro release	353:372	the in vitro release of ciprofloxacin drugs	353:395	The present work involves the designing of the porous nano gold embedded cellulose grafted polyacrylamide (PAM/C/Au) nanocomposite hydrogel which has been prepared by in situ polymerization process with an objective of application for the in vitro release of ciprofloxacin drugs.
30597238	5	69	theme	cytotoxicity	950:961	arg1	tests					963:967	cytotoxicity tests	950:967	cytotoxicity tests	950:967	The investigations of cytotoxicity tests and antibacterial behaviour along with negative and positive actions of nanocomposite hydrogels are investigated.
30597238	7	70	theme	drug	1286:1289	arg1	%					1310:1310	96.6%	1306:1310	96.6%	1306:1310	The in vitro release rate of ciprofloxacin antibiotic drug is found to be 96.6% in 5 h.
30597238	7	70	theme	drug	1286:1289	arg1	rate					1253:1256	The in vitro release rate	1232:1256	The in vitro release rate of ciprofloxacin antibiotic drug	1232:1289	The in vitro release rate of ciprofloxacin antibiotic drug is found to be 96.6% in 5 h.
30597238	2	71	theme	electron	640:647	arg1	microscopy					649:658	field emission scanning electron microscopy	616:658	field emission scanning electron microscopy (FESEM)	616:666	The structure, composition, morphology behaviour of the nanocomposite hydrogels are explored using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction study (XRD), X-ray photoelectron spectroscopy (XPS); field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and atomic force microscopy (AFM).
30597238	2	71	theme	electron	640:647	arg1	FESEM					661:665	FESEM	661:665	FESEM	661:665	The structure, composition, morphology behaviour of the nanocomposite hydrogels are explored using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction study (XRD), X-ray photoelectron spectroscopy (XPS); field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and atomic force microscopy (AFM).
30597238	8	72	theme	nanocomposite	1333:1345	arg1	proposed					1419:1426	proposed	1419:1426	proposed as a good carrier towards in vitro release of the ciprofloxacin drugs	1419:1496	The PAM/C/Au nanocomposite hydrogels with improved thermal and rheological properties are suitable proposed as a good carrier towards in vitro release of the ciprofloxacin drugs.
30597238	8	72	theme	nanocomposite	1333:1345	arg1	hydrogels					1347:1355	The PAM/C/Au nanocomposite hydrogels	1320:1355	The PAM/C/Au nanocomposite hydrogels with improved thermal and rheological properties	1320:1404	The PAM/C/Au nanocomposite hydrogels with improved thermal and rheological properties are suitable proposed as a good carrier towards in vitro release of the ciprofloxacin drugs.
30597238	8	72	theme	nanocomposite	1333:1345	arg1	suitable					1410:1417	suitable	1410:1417	suitable	1410:1417	The PAM/C/Au nanocomposite hydrogels with improved thermal and rheological properties are suitable proposed as a good carrier towards in vitro release of the ciprofloxacin drugs.
30597238	3	73	theme	hydrogels	801:809	arg1	stability					755:763	The thermal stability	743:763	The thermal stability of the as synthesized nanocomposite hydrogels	743:809	The thermal stability of the as synthesized nanocomposite hydrogels are studied by TGA.
30597238	2	74	theme	transmission	669:680	arg1	TEM					703:705	TEM	703:705	TEM	703:705	The structure, composition, morphology behaviour of the nanocomposite hydrogels are explored using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction study (XRD), X-ray photoelectron spectroscopy (XPS); field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and atomic force microscopy (AFM).
30597238	2	74	theme	transmission	669:680	arg1	microscopy					691:700	transmission electron microscopy	669:700	transmission electron microscopy (TEM)	669:706	The structure, composition, morphology behaviour of the nanocomposite hydrogels are explored using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction study (XRD), X-ray photoelectron spectroscopy (XPS); field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and atomic force microscopy (AFM).
30597238	1	75	theme	ciprofloxacin	377:389	arg1	drugs					391:395	ciprofloxacin drugs	377:395	ciprofloxacin drugs	377:395	The present work involves the designing of the porous nano gold embedded cellulose grafted polyacrylamide (PAM/C/Au) nanocomposite hydrogel which has been prepared by in situ polymerization process with an objective of application for the in vitro release of ciprofloxacin drugs.
30597238	0	76	theme	drugs	25:29	arg1	Release					0:6	Release	0:6	Release of ciprofloxacin drugs by nano gold embedded cellulose	0:61	Release of ciprofloxacin drugs by nano gold embedded cellulose grafted polyacrylamide hybrid nanocomposite hydrogels.
30597238	7	77	dep	in	1236:1237	arg1	vitro					1239:1243	vitro	1239:1243	vitro	1239:1243	The in vitro release rate of ciprofloxacin antibiotic drug is found to be 96.6% in 5 h.
30597238	8	78	theme	rheological	1383:1393	arg1	properties					1395:1404	improved thermal and rheological properties	1362:1404	properties	1395:1404	The PAM/C/Au nanocomposite hydrogels with improved thermal and rheological properties are suitable proposed as a good carrier towards in vitro release of the ciprofloxacin drugs.
30597238	0	79	theme	nano	34:37	arg1	cellulose					53:61	nano gold embedded cellulose	34:61	nano gold embedded cellulose	34:61	Release of ciprofloxacin drugs by nano gold embedded cellulose grafted polyacrylamide hybrid nanocomposite hydrogels.
30597238	0	80	theme	embedded	44:51	arg1	cellulose					53:61	nano gold embedded cellulose	34:61	nano gold embedded cellulose	34:61	Release of ciprofloxacin drugs by nano gold embedded cellulose grafted polyacrylamide hybrid nanocomposite hydrogels.
30597238	2	81	theme	hydrogels	468:476	arg1	composition					413:423	composition	413:423	composition	413:423	The structure, composition, morphology behaviour of the nanocomposite hydrogels are explored using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction study (XRD), X-ray photoelectron spectroscopy (XPS); field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and atomic force microscopy (AFM).
30597238	2	81	theme	hydrogels	468:476	arg1	behaviour					437:445	morphology behaviour	426:445	morphology behaviour	426:445	The structure, composition, morphology behaviour of the nanocomposite hydrogels are explored using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction study (XRD), X-ray photoelectron spectroscopy (XPS); field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and atomic force microscopy (AFM).
30597238	2	81	theme	hydrogels	468:476	arg1	structure					402:410	The structure	398:410	The structure	398:410	The structure, composition, morphology behaviour of the nanocomposite hydrogels are explored using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction study (XRD), X-ray photoelectron spectroscopy (XPS); field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and atomic force microscopy (AFM).
30597238	8	82	dep	in	1454:1455	arg1	vitro					1457:1461	vitro	1457:1461	vitro	1457:1461	The PAM/C/Au nanocomposite hydrogels with improved thermal and rheological properties are suitable proposed as a good carrier towards in vitro release of the ciprofloxacin drugs.
30597238	5	83	theme	behaviour	987:995	arg1	investigations					932:945	The investigations	928:945	The investigations of cytotoxicity tests and antibacterial behaviour along with negative and positive actions of nanocomposite hydrogels	928:1063	The investigations of cytotoxicity tests and antibacterial behaviour along with negative and positive actions of nanocomposite hydrogels are investigated.
30597238	6	84	theme	drugs	1130:1134	arg1	rate					1108:1111	the release rate	1096:1111	the release rate of ciprofloxacin drugs	1096:1134	The study of the release rate of ciprofloxacin drugs is carried out by measuring water retention and swelling properties of nanocomposite hydrogels.
30597238	6	85	theme	rate	1108:1111	arg1	study					1087:1091	The study	1083:1091	The study of the release rate of ciprofloxacin drugs	1083:1134	The study of the release rate of ciprofloxacin drugs is carried out by measuring water retention and swelling properties of nanocomposite hydrogels.
30597238	1	86	theme	nanocomposite	235:247	arg1	hydrogel					249:256	the porous nano gold embedded cellulose grafted polyacrylamide (PAM/C/Au) nanocomposite hydrogel	161:256	the porous nano gold embedded cellulose grafted polyacrylamide (PAM/C/Au) nanocomposite hydrogel which has been prepared by in situ polymerization process with an objective of application for the in vitro release of ciprofloxacin drugs	161:395	The present work involves the designing of the porous nano gold embedded cellulose grafted polyacrylamide (PAM/C/Au) nanocomposite hydrogel which has been prepared by in situ polymerization process with an objective of application for the in vitro release of ciprofloxacin drugs.
30597238	7	87	theme	ciprofloxacin	1261:1273	arg1	drug					1286:1289	ciprofloxacin antibiotic drug	1261:1289	ciprofloxacin antibiotic drug	1261:1289	The in vitro release rate of ciprofloxacin antibiotic drug is found to be 96.6% in 5 h.
30900531	0	0	theme	Derived	84:90	arg1	Microvesicles					106:118	Their Derived Extracellular Microvesicles	78:118	Their Derived Extracellular Microvesicles	78:118	Glycan Profiling Analysis of Equine Amniotic Progenitor Mesenchymal Cells and Their Derived Extracellular Microvesicles.
30900531	8	1	dep	eAMCs	1046:1050	arg1	respect					1035:1041	respect	1035:1041	respect	1035:1041	With respect to eAMCs, eAMC-EVs, were found to be (1) enriched in Galβ1,3GalNAc terminating O-glycans, α2,3-linked sialoglycans, and high-mannose N-glycans (Con A); (2) diminished in N-acetyllactosamine, GalNAc, Gal, GlcNAc, and fucose terminating glycans; and (3) unchanged in α2,6 linked sialoglycans content.
30900531	9	2	theme	eAMC-EVs	1559:1566	arg1	markers					1544:1550	markers	1544:1550	markers of the eAMC-EVs	1544:1566	These results suggest that eAMC-EVs emerge from a specific eAMC microdomain, and that the high simultaneous presence of Galβ1,3GalNAc, α2,3 sialic acid, and high-mannose N-linked glycans may constitute markers of the eAMC-EVs.
30900531	9	3	theme	glycans	1521:1527	arg1	presence					1450:1457	the high simultaneous presence	1428:1457	the high simultaneous presence of Galβ1,3GalNAc, α2,3 sialic acid, and high-mannose N-linked glycans	1428:1527	These results suggest that eAMC-EVs emerge from a specific eAMC microdomain, and that the high simultaneous presence of Galβ1,3GalNAc, α2,3 sialic acid, and high-mannose N-linked glycans may constitute markers of the eAMC-EVs.
30900531	2	4	theme	equine	355:360	arg1	lesions					369:375	equine tendon lesions	355:375	equine tendon lesions	355:375	Their secreted vesicles are important effectors in a wide array of biological processes, and contribute to in vivo healing of equine tendon lesions and endometrial inflammation.
30900531	4	5	theme	glycosylation	580:592	arg1	pattern					594:600	the surface glycosylation pattern	568:600	the surface glycosylation pattern of eAMCs	568:609	In this study, we evaluated the surface glycosylation pattern of eAMCs and their EVs from the eAMCs released in conditioned medium.
30900531	9	6	theme	N-linked	1512:1519	arg1	glycans					1521:1527	high-mannose N-linked glycans	1499:1527	high-mannose N-linked glycans	1499:1527	These results suggest that eAMC-EVs emerge from a specific eAMC microdomain, and that the high simultaneous presence of Galβ1,3GalNAc, α2,3 sialic acid, and high-mannose N-linked glycans may constitute markers of the eAMC-EVs.
30900531	10	7	theme	further	1635:1641	arg1	investigation					1643:1655	further investigation	1635:1655	further investigation	1635:1655	The role of these sugars in equine regenerative medicine requires further investigation.
30900531	4	8	theme	surface	572:578	arg1	pattern					594:600	the surface glycosylation pattern	568:600	the surface glycosylation pattern of eAMCs	568:609	In this study, we evaluated the surface glycosylation pattern of eAMCs and their EVs from the eAMCs released in conditioned medium.
30900531	0	9	theme	Microvesicles	106:118	arg1	Analysis					17:24	Glycan Profiling Analysis	0:24	Glycan Profiling Analysis of Equine Amniotic Progenitor Mesenchymal Cells and Their Derived Extracellular Microvesicles.	0:119	Glycan Profiling Analysis of Equine Amniotic Progenitor Mesenchymal Cells and Their Derived Extracellular Microvesicles.
30900531	9	10	theme	high	1432:1435	arg1	presence					1450:1457	the high simultaneous presence	1428:1457	the high simultaneous presence of Galβ1,3GalNAc, α2,3 sialic acid, and high-mannose N-linked glycans	1428:1527	These results suggest that eAMC-EVs emerge from a specific eAMC microdomain, and that the high simultaneous presence of Galβ1,3GalNAc, α2,3 sialic acid, and high-mannose N-linked glycans may constitute markers of the eAMC-EVs.
30900531	3	11	theme	recipient	523:531	arg1	cells					533:537	recipient cells	523:537	recipient cells	523:537	Glycoconjugates are involved in cellular recognition and in the efficient uptake of extracellular vesicles (EVs) by recipient cells.
30900531	2	12	theme	biological	296:305	arg1	processes					307:315	biological processes	296:315	biological processes	296:315	Their secreted vesicles are important effectors in a wide array of biological processes, and contribute to in vivo healing of equine tendon lesions and endometrial inflammation.
30900531	2	13	theme	processes	307:315	arg1	array					287:291	a wide array	280:291	a wide array of biological processes	280:315	Their secreted vesicles are important effectors in a wide array of biological processes, and contribute to in vivo healing of equine tendon lesions and endometrial inflammation.
30900531	0	14	theme	Extracellular	92:104	arg1	Microvesicles					106:118	Their Derived Extracellular Microvesicles	78:118	Their Derived Extracellular Microvesicles	78:118	Glycan Profiling Analysis of Equine Amniotic Progenitor Mesenchymal Cells and Their Derived Extracellular Microvesicles.
30900531	6	15	theme	microarray	891:900	arg1	scanner					902:908	a microarray scanner	889:908	a microarray scanner	889:908	Signal intensity was detected using a microarray scanner.
30900531	9	16	theme	Galβ1,3GalNAc	1462:1474	arg1	presence					1450:1457	the high simultaneous presence	1428:1457	the high simultaneous presence of Galβ1,3GalNAc, α2,3 sialic acid, and high-mannose N-linked glycans	1428:1527	These results suggest that eAMC-EVs emerge from a specific eAMC microdomain, and that the high simultaneous presence of Galβ1,3GalNAc, α2,3 sialic acid, and high-mannose N-linked glycans may constitute markers of the eAMC-EVs.
30900531	9	17	theme	α2,3	1477:1480	arg1	acid					1489:1492	α2,3 sialic acid	1477:1492	α2,3 sialic acid	1477:1492	These results suggest that eAMC-EVs emerge from a specific eAMC microdomain, and that the high simultaneous presence of Galβ1,3GalNAc, α2,3 sialic acid, and high-mannose N-linked glycans may constitute markers of the eAMC-EVs.
30900531	10	18	theme	equine	1597:1602	arg1	medicine					1617:1624	equine regenerative medicine	1597:1624	equine regenerative medicine	1597:1624	The role of these sugars in equine regenerative medicine requires further investigation.
30900531	10	19	theme	sugars	1587:1592	arg1	role					1573:1576	The role	1569:1576	The role of these sugars in equine regenerative medicine	1569:1624	The role of these sugars in equine regenerative medicine requires further investigation.
30900531	3	20	theme	cellular	439:446	arg1	recognition					448:458	cellular recognition	439:458	cellular recognition	439:458	Glycoconjugates are involved in cellular recognition and in the efficient uptake of extracellular vesicles (EVs) by recipient cells.
30900531	4	21	theme	conditioned	652:662	arg1	medium					664:669	conditioned medium	652:669	conditioned medium	652:669	In this study, we evaluated the surface glycosylation pattern of eAMCs and their EVs from the eAMCs released in conditioned medium.
30900531	8	22	theme	α2,3-linked	1133:1143	arg1	sialoglycans					1145:1156	α2,3-linked sialoglycans	1133:1156	α2,3-linked sialoglycans	1133:1156	With respect to eAMCs, eAMC-EVs, were found to be (1) enriched in Galβ1,3GalNAc terminating O-glycans, α2,3-linked sialoglycans, and high-mannose N-glycans (Con A); (2) diminished in N-acetyllactosamine, GalNAc, Gal, GlcNAc, and fucose terminating glycans; and (3) unchanged in α2,6 linked sialoglycans content.
30900531	10	23	theme	regenerative	1604:1615	arg1	medicine					1617:1624	equine regenerative medicine	1597:1624	equine regenerative medicine	1597:1624	The role of these sugars in equine regenerative medicine requires further investigation.
30900531	8	24	theme	Con	1187:1189	arg1	A					1191:1191	Con A	1187:1191	Con A	1187:1191	With respect to eAMCs, eAMC-EVs, were found to be (1) enriched in Galβ1,3GalNAc terminating O-glycans, α2,3-linked sialoglycans, and high-mannose N-glycans (Con A); (2) diminished in N-acetyllactosamine, GalNAc, Gal, GlcNAc, and fucose terminating glycans; and (3) unchanged in α2,6 linked sialoglycans content.
30900531	8	24	theme	Con	1187:1189	arg1	O-glycans					1122:1130	O-glycans	1122:1130	O-glycans	1122:1130	With respect to eAMCs, eAMC-EVs, were found to be (1) enriched in Galβ1,3GalNAc terminating O-glycans, α2,3-linked sialoglycans, and high-mannose N-glycans (Con A); (2) diminished in N-acetyllactosamine, GalNAc, Gal, GlcNAc, and fucose terminating glycans; and (3) unchanged in α2,6 linked sialoglycans content.
30900531	2	25	theme	wide	282:285	arg1	array					287:291	a wide array	280:291	a wide array of biological processes	280:315	Their secreted vesicles are important effectors in a wide array of biological processes, and contribute to in vivo healing of equine tendon lesions and endometrial inflammation.
30900531	1	26	theme	many	179:182	arg1	mechanisms					184:193	many mechanisms	179:193	many mechanisms in tissue regenerative processes	179:226	Equine amniotic mesenchymal cells (eAMCs) are involved in many mechanisms in tissue regenerative processes.
30900531	0	27	theme	Profiling	7:15	arg1	Analysis					17:24	Glycan Profiling Analysis	0:24	Glycan Profiling Analysis of Equine Amniotic Progenitor Mesenchymal Cells and Their Derived Extracellular Microvesicles.	0:119	Glycan Profiling Analysis of Equine Amniotic Progenitor Mesenchymal Cells and Their Derived Extracellular Microvesicles.
30900531	9	28	theme	simultaneous	1437:1448	arg1	presence					1450:1457	the high simultaneous presence	1428:1457	the high simultaneous presence of Galβ1,3GalNAc, α2,3 sialic acid, and high-mannose N-linked glycans	1428:1527	These results suggest that eAMC-EVs emerge from a specific eAMC microdomain, and that the high simultaneous presence of Galβ1,3GalNAc, α2,3 sialic acid, and high-mannose N-linked glycans may constitute markers of the eAMC-EVs.
30900531	4	29	gly	glycosylation	580:592	arg1	eAMCs					605:609	eAMCs	605:609	eAMCs	605:609	In this study, we evaluated the surface glycosylation pattern of eAMCs and their EVs from the eAMCs released in conditioned medium.
30900531	2	30	dep	in	336:337	arg1	vivo					339:342	vivo	339:342	vivo	339:342	Their secreted vesicles are important effectors in a wide array of biological processes, and contribute to in vivo healing of equine tendon lesions and endometrial inflammation.
30900531	2	31	theme	endometrial	381:391	arg1	inflammation					393:404	endometrial inflammation	381:404	endometrial inflammation	381:404	Their secreted vesicles are important effectors in a wide array of biological processes, and contribute to in vivo healing of equine tendon lesions and endometrial inflammation.
30900531	0	32	theme	Glycan	0:5	arg1	Analysis					17:24	Glycan Profiling Analysis	0:24	Glycan Profiling Analysis of Equine Amniotic Progenitor Mesenchymal Cells and Their Derived Extracellular Microvesicles.	0:119	Glycan Profiling Analysis of Equine Amniotic Progenitor Mesenchymal Cells and Their Derived Extracellular Microvesicles.
30900531	8	33	dep	enriched	1084:1091	arg1	1					1081:1081	1	1081:1081	1	1081:1081	With respect to eAMCs, eAMC-EVs, were found to be (1) enriched in Galβ1,3GalNAc terminating O-glycans, α2,3-linked sialoglycans, and high-mannose N-glycans (Con A); (2) diminished in N-acetyllactosamine, GalNAc, Gal, GlcNAc, and fucose terminating glycans; and (3) unchanged in α2,6 linked sialoglycans content.
30900531	9	34	theme	acid	1489:1492	arg1	presence					1450:1457	the high simultaneous presence	1428:1457	the high simultaneous presence of Galβ1,3GalNAc, α2,3 sialic acid, and high-mannose N-linked glycans	1428:1527	These results suggest that eAMC-EVs emerge from a specific eAMC microdomain, and that the high simultaneous presence of Galβ1,3GalNAc, α2,3 sialic acid, and high-mannose N-linked glycans may constitute markers of the eAMC-EVs.
30900531	2	35	from	effectors	267:275	arg1	array					287:291	a wide array	280:291	a wide array of biological processes	280:315	Their secreted vesicles are important effectors in a wide array of biological processes, and contribute to in vivo healing of equine tendon lesions and endometrial inflammation.
30900531	4	36	theme	eAMCs	605:609	arg1	pattern					594:600	the surface glycosylation pattern	568:600	the surface glycosylation pattern of eAMCs	568:609	In this study, we evaluated the surface glycosylation pattern of eAMCs and their EVs from the eAMCs released in conditioned medium.
30900531	4	36	theme	eAMCs	605:609	arg1	EVs					621:623	their EVs	615:623	their EVs from the eAMCs released in conditioned medium	615:669	In this study, we evaluated the surface glycosylation pattern of eAMCs and their EVs from the eAMCs released in conditioned medium.
30900531	7	37	theme	eAMC-EV	925:931	arg1	microarrays					933:943	eAMC-EV microarrays	925:943	eAMC-EV microarrays	925:943	Both eAMC and eAMC-EV microarrays interacted with all the lectins, indicating the presence of N- and O-linked glycans.
30900531	4	38	from	eAMCs	634:638	arg1	pattern					594:600	the surface glycosylation pattern	568:600	the surface glycosylation pattern of eAMCs	568:609	In this study, we evaluated the surface glycosylation pattern of eAMCs and their EVs from the eAMCs released in conditioned medium.
30900531	4	38	from	eAMCs	634:638	arg1	EVs					621:623	their EVs	615:623	their EVs from the eAMCs released in conditioned medium	615:669	In this study, we evaluated the surface glycosylation pattern of eAMCs and their EVs from the eAMCs released in conditioned medium.
30900531	1	39	theme	tissue	198:203	arg1	processes					218:226	tissue regenerative processes	198:226	tissue regenerative processes	198:226	Equine amniotic mesenchymal cells (eAMCs) are involved in many mechanisms in tissue regenerative processes.
30900531	5	40	theme	biotinylated	799:810	arg1	lectins					812:818	14 biotinylated lectins	796:818	14 biotinylated lectins	796:818	We used a microarray procedure in which eAMCs and eAMC-EVs were spotted on microarray slides, and incubated with a panel of 14 biotinylated lectins and Cy3-conjugated streptavidin.
30900531	1	41	theme	Equine	121:126	arg1	cells					149:153	Equine amniotic mesenchymal cells	121:153	Equine amniotic mesenchymal cells (eAMCs)	121:161	Equine amniotic mesenchymal cells (eAMCs) are involved in many mechanisms in tissue regenerative processes.
30900531	1	41	theme	Equine	121:126	arg1	eAMCs					156:160	eAMCs	156:160	eAMCs	156:160	Equine amniotic mesenchymal cells (eAMCs) are involved in many mechanisms in tissue regenerative processes.
30900531	8	42	link	linked	1313:1318	arg1	content					1333:1339	linked sialoglycans content	1313:1339	linked sialoglycans content	1313:1339	With respect to eAMCs, eAMC-EVs, were found to be (1) enriched in Galβ1,3GalNAc terminating O-glycans, α2,3-linked sialoglycans, and high-mannose N-glycans (Con A); (2) diminished in N-acetyllactosamine, GalNAc, Gal, GlcNAc, and fucose terminating glycans; and (3) unchanged in α2,6 linked sialoglycans content.
30900531	1	43	theme	regenerative	205:216	arg1	processes					218:226	tissue regenerative processes	198:226	tissue regenerative processes	198:226	Equine amniotic mesenchymal cells (eAMCs) are involved in many mechanisms in tissue regenerative processes.
30900531	0	44	theme	Amniotic	36:43	arg1	Cells					68:72	Equine Amniotic Progenitor Mesenchymal Cells	29:72	Equine Amniotic Progenitor Mesenchymal Cells	29:72	Glycan Profiling Analysis of Equine Amniotic Progenitor Mesenchymal Cells and Their Derived Extracellular Microvesicles.
30900531	9	45	theme	sialic	1482:1487	arg1	acid					1489:1492	α2,3 sialic acid	1477:1492	α2,3 sialic acid	1477:1492	These results suggest that eAMC-EVs emerge from a specific eAMC microdomain, and that the high simultaneous presence of Galβ1,3GalNAc, α2,3 sialic acid, and high-mannose N-linked glycans may constitute markers of the eAMC-EVs.
30900531	9	46	theme	specific	1392:1399	arg1	microdomain					1406:1416	a specific eAMC microdomain	1390:1416	a specific eAMC microdomain	1390:1416	These results suggest that eAMC-EVs emerge from a specific eAMC microdomain, and that the high simultaneous presence of Galβ1,3GalNAc, α2,3 sialic acid, and high-mannose N-linked glycans may constitute markers of the eAMC-EVs.
30900531	9	47	link	N-linked	1512:1519	arg1	glycans					1521:1527	high-mannose N-linked glycans	1499:1527	high-mannose N-linked glycans	1499:1527	These results suggest that eAMC-EVs emerge from a specific eAMC microdomain, and that the high simultaneous presence of Galβ1,3GalNAc, α2,3 sialic acid, and high-mannose N-linked glycans may constitute markers of the eAMC-EVs.
30900531	8	48	theme	high-mannose	1163:1174	arg1	N-glycans					1176:1184	high-mannose N-glycans	1163:1184	high-mannose N-glycans	1163:1184	With respect to eAMCs, eAMC-EVs, were found to be (1) enriched in Galβ1,3GalNAc terminating O-glycans, α2,3-linked sialoglycans, and high-mannose N-glycans (Con A); (2) diminished in N-acetyllactosamine, GalNAc, Gal, GlcNAc, and fucose terminating glycans; and (3) unchanged in α2,6 linked sialoglycans content.
30900531	5	49	theme	Cy3-conjugated	824:837	arg1	streptavidin					839:850	Cy3-conjugated streptavidin	824:850	Cy3-conjugated streptavidin	824:850	We used a microarray procedure in which eAMCs and eAMC-EVs were spotted on microarray slides, and incubated with a panel of 14 biotinylated lectins and Cy3-conjugated streptavidin.
30900531	0	50	theme	Equine	29:34	arg1	Cells					68:72	Equine Amniotic Progenitor Mesenchymal Cells	29:72	Equine Amniotic Progenitor Mesenchymal Cells	29:72	Glycan Profiling Analysis of Equine Amniotic Progenitor Mesenchymal Cells and Their Derived Extracellular Microvesicles.
30900531	6	51	theme	Signal	853:858	arg1	intensity					860:868	Signal intensity	853:868	Signal intensity	853:868	Signal intensity was detected using a microarray scanner.
30900531	5	52	used	used	675:678	arg2	We					672:673	We	672:673	We	672:673	We used a microarray procedure in which eAMCs and eAMC-EVs were spotted on microarray slides, and incubated with a panel of 14 biotinylated lectins and Cy3-conjugated streptavidin.
30900531	3	53	theme	efficient	471:479	arg1	uptake					481:486	the efficient uptake	467:486	the efficient uptake of extracellular vesicles (EVs) by recipient cells	467:537	Glycoconjugates are involved in cellular recognition and in the efficient uptake of extracellular vesicles (EVs) by recipient cells.
30900531	0	54	theme	Mesenchymal	56:66	arg1	Cells					68:72	Equine Amniotic Progenitor Mesenchymal Cells	29:72	Equine Amniotic Progenitor Mesenchymal Cells	29:72	Glycan Profiling Analysis of Equine Amniotic Progenitor Mesenchymal Cells and Their Derived Extracellular Microvesicles.
30900531	5	55	theme	microarray	747:756	arg1	slides					758:763	microarray slides	747:763	microarray slides	747:763	We used a microarray procedure in which eAMCs and eAMC-EVs were spotted on microarray slides, and incubated with a panel of 14 biotinylated lectins and Cy3-conjugated streptavidin.
30900531	2	56	theme	secreted	235:242	arg1	vesicles					244:251	Their secreted vesicles	229:251	Their secreted vesicles	229:251	Their secreted vesicles are important effectors in a wide array of biological processes, and contribute to in vivo healing of equine tendon lesions and endometrial inflammation.
30900531	2	56	theme	secreted	235:242	arg1	effectors					267:275	important effectors	257:275	important effectors	257:275	Their secreted vesicles are important effectors in a wide array of biological processes, and contribute to in vivo healing of equine tendon lesions and endometrial inflammation.
30900531	7	57	theme	O-linked	1012:1019	arg1	glycans					1021:1027	N- and O-linked glycans	1005:1027	N- and O-linked glycans	1005:1027	Both eAMC and eAMC-EV microarrays interacted with all the lectins, indicating the presence of N- and O-linked glycans.
30900531	1	58	theme	amniotic	128:135	arg1	cells					149:153	Equine amniotic mesenchymal cells	121:153	Equine amniotic mesenchymal cells (eAMCs)	121:161	Equine amniotic mesenchymal cells (eAMCs) are involved in many mechanisms in tissue regenerative processes.
30900531	1	58	theme	amniotic	128:135	arg1	eAMCs					156:160	eAMCs	156:160	eAMCs	156:160	Equine amniotic mesenchymal cells (eAMCs) are involved in many mechanisms in tissue regenerative processes.
30900531	0	59	theme	Progenitor	45:54	arg1	Cells					68:72	Equine Amniotic Progenitor Mesenchymal Cells	29:72	Equine Amniotic Progenitor Mesenchymal Cells	29:72	Glycan Profiling Analysis of Equine Amniotic Progenitor Mesenchymal Cells and Their Derived Extracellular Microvesicles.
30900531	8	60	theme	sialoglycans	1320:1331	arg1	content					1333:1339	linked sialoglycans content	1313:1339	linked sialoglycans content	1313:1339	With respect to eAMCs, eAMC-EVs, were found to be (1) enriched in Galβ1,3GalNAc terminating O-glycans, α2,3-linked sialoglycans, and high-mannose N-glycans (Con A); (2) diminished in N-acetyllactosamine, GalNAc, Gal, GlcNAc, and fucose terminating glycans; and (3) unchanged in α2,6 linked sialoglycans content.
30900531	8	61	theme	linked	1313:1318	arg1	content					1333:1339	linked sialoglycans content	1313:1339	linked sialoglycans content	1313:1339	With respect to eAMCs, eAMC-EVs, were found to be (1) enriched in Galβ1,3GalNAc terminating O-glycans, α2,3-linked sialoglycans, and high-mannose N-glycans (Con A); (2) diminished in N-acetyllactosamine, GalNAc, Gal, GlcNAc, and fucose terminating glycans; and (3) unchanged in α2,6 linked sialoglycans content.
30900531	10	62	from	role	1573:1576	arg1	medicine					1617:1624	equine regenerative medicine	1597:1624	equine regenerative medicine	1597:1624	The role of these sugars in equine regenerative medicine requires further investigation.
30900531	1	63	theme	mesenchymal	137:147	arg1	cells					149:153	Equine amniotic mesenchymal cells	121:153	Equine amniotic mesenchymal cells (eAMCs)	121:161	Equine amniotic mesenchymal cells (eAMCs) are involved in many mechanisms in tissue regenerative processes.
30900531	1	63	theme	mesenchymal	137:147	arg1	eAMCs					156:160	eAMCs	156:160	eAMCs	156:160	Equine amniotic mesenchymal cells (eAMCs) are involved in many mechanisms in tissue regenerative processes.
30900531	7	64	link	O-linked	1012:1019	arg1	glycans					1021:1027	N- and O-linked glycans	1005:1027	N- and O-linked glycans	1005:1027	Both eAMC and eAMC-EV microarrays interacted with all the lectins, indicating the presence of N- and O-linked glycans.
30900531	7	65	theme	glycans	1021:1027	arg1	presence					993:1000	the presence	989:1000	the presence of N- and O-linked glycans	989:1027	Both eAMC and eAMC-EV microarrays interacted with all the lectins, indicating the presence of N- and O-linked glycans.
30900531	7	66	theme	N-	1005:1006	arg1	glycans					1021:1027	N- and O-linked glycans	1005:1027	N- and O-linked glycans	1005:1027	Both eAMC and eAMC-EV microarrays interacted with all the lectins, indicating the presence of N- and O-linked glycans.
30900531	2	67	theme	in	336:337	arg1	healing					344:350	in vivo healing	336:350	in vivo healing of equine tendon lesions	336:375	Their secreted vesicles are important effectors in a wide array of biological processes, and contribute to in vivo healing of equine tendon lesions and endometrial inflammation.
30900531	5	68	theme	microarray	682:691	arg1	procedure					693:701	a microarray procedure	680:701	a microarray procedure in which eAMCs and eAMC-EVs were spotted on microarray slides, and incubated with a panel of 14 biotinylated lectins and Cy3-conjugated streptavidin	680:850	We used a microarray procedure in which eAMCs and eAMC-EVs were spotted on microarray slides, and incubated with a panel of 14 biotinylated lectins and Cy3-conjugated streptavidin.
30900531	1	69	from	mechanisms	184:193	arg1	processes					218:226	tissue regenerative processes	198:226	tissue regenerative processes	198:226	Equine amniotic mesenchymal cells (eAMCs) are involved in many mechanisms in tissue regenerative processes.
30900531	3	70	theme	vesicles	505:512	arg1	uptake					481:486	the efficient uptake	467:486	the efficient uptake of extracellular vesicles (EVs) by recipient cells	467:537	Glycoconjugates are involved in cellular recognition and in the efficient uptake of extracellular vesicles (EVs) by recipient cells.
30900531	3	71	theme	extracellular	491:503	arg1	EVs					515:517	EVs	515:517	EVs	515:517	Glycoconjugates are involved in cellular recognition and in the efficient uptake of extracellular vesicles (EVs) by recipient cells.
30900531	3	71	theme	extracellular	491:503	arg1	vesicles					505:512	extracellular vesicles	491:512	extracellular vesicles (EVs)	491:518	Glycoconjugates are involved in cellular recognition and in the efficient uptake of extracellular vesicles (EVs) by recipient cells.
30900531	9	72	theme	eAMC	1401:1404	arg1	microdomain					1406:1416	a specific eAMC microdomain	1390:1416	a specific eAMC microdomain	1390:1416	These results suggest that eAMC-EVs emerge from a specific eAMC microdomain, and that the high simultaneous presence of Galβ1,3GalNAc, α2,3 sialic acid, and high-mannose N-linked glycans may constitute markers of the eAMC-EVs.
30900531	5	73	theme	streptavidin	839:850	arg1	panel					787:791	a panel	785:791	a panel of 14 biotinylated lectins and Cy3-conjugated streptavidin	785:850	We used a microarray procedure in which eAMCs and eAMC-EVs were spotted on microarray slides, and incubated with a panel of 14 biotinylated lectins and Cy3-conjugated streptavidin.
30900531	8	74	dep	diminished	1199:1208	arg1	Galβ1,3GalNAc					1096:1108	Galβ1,3GalNAc	1096:1108	Galβ1,3GalNAc terminating O-glycans, α2,3-linked sialoglycans, and high-mannose N-glycans (Con A)	1096:1192	With respect to eAMCs, eAMC-EVs, were found to be (1) enriched in Galβ1,3GalNAc terminating O-glycans, α2,3-linked sialoglycans, and high-mannose N-glycans (Con A); (2) diminished in N-acetyllactosamine, GalNAc, Gal, GlcNAc, and fucose terminating glycans; and (3) unchanged in α2,6 linked sialoglycans content.
30900531	8	74	dep	diminished	1199:1208	arg1	2					1196:1196	2	1196:1196	2	1196:1196	With respect to eAMCs, eAMC-EVs, were found to be (1) enriched in Galβ1,3GalNAc terminating O-glycans, α2,3-linked sialoglycans, and high-mannose N-glycans (Con A); (2) diminished in N-acetyllactosamine, GalNAc, Gal, GlcNAc, and fucose terminating glycans; and (3) unchanged in α2,6 linked sialoglycans content.
30900531	5	75	theme	lectins	812:818	arg1	panel					787:791	a panel	785:791	a panel of 14 biotinylated lectins and Cy3-conjugated streptavidin	785:850	We used a microarray procedure in which eAMCs and eAMC-EVs were spotted on microarray slides, and incubated with a panel of 14 biotinylated lectins and Cy3-conjugated streptavidin.
30900531	2	76	theme	lesions	369:375	arg1	inflammation					393:404	endometrial inflammation	381:404	endometrial inflammation	381:404	Their secreted vesicles are important effectors in a wide array of biological processes, and contribute to in vivo healing of equine tendon lesions and endometrial inflammation.
30900531	2	76	theme	lesions	369:375	arg1	healing					344:350	in vivo healing	336:350	in vivo healing of equine tendon lesions	336:375	Their secreted vesicles are important effectors in a wide array of biological processes, and contribute to in vivo healing of equine tendon lesions and endometrial inflammation.
30900531	8	77	link	α2,3-linked	1133:1143	arg1	sialoglycans					1145:1156	α2,3-linked sialoglycans	1133:1156	α2,3-linked sialoglycans	1133:1156	With respect to eAMCs, eAMC-EVs, were found to be (1) enriched in Galβ1,3GalNAc terminating O-glycans, α2,3-linked sialoglycans, and high-mannose N-glycans (Con A); (2) diminished in N-acetyllactosamine, GalNAc, Gal, GlcNAc, and fucose terminating glycans; and (3) unchanged in α2,6 linked sialoglycans content.
30900531	9	78	theme	high-mannose	1499:1510	arg1	glycans					1521:1527	high-mannose N-linked glycans	1499:1527	high-mannose N-linked glycans	1499:1527	These results suggest that eAMC-EVs emerge from a specific eAMC microdomain, and that the high simultaneous presence of Galβ1,3GalNAc, α2,3 sialic acid, and high-mannose N-linked glycans may constitute markers of the eAMC-EVs.
30900531	0	79	theme	Cells	68:72	arg1	Analysis					17:24	Glycan Profiling Analysis	0:24	Glycan Profiling Analysis of Equine Amniotic Progenitor Mesenchymal Cells and Their Derived Extracellular Microvesicles.	0:119	Glycan Profiling Analysis of Equine Amniotic Progenitor Mesenchymal Cells and Their Derived Extracellular Microvesicles.
30900531	2	80	theme	important	257:265	arg1	vesicles					244:251	Their secreted vesicles	229:251	Their secreted vesicles	229:251	Their secreted vesicles are important effectors in a wide array of biological processes, and contribute to in vivo healing of equine tendon lesions and endometrial inflammation.
30900531	2	80	theme	important	257:265	arg1	effectors					267:275	important effectors	257:275	important effectors	257:275	Their secreted vesicles are important effectors in a wide array of biological processes, and contribute to in vivo healing of equine tendon lesions and endometrial inflammation.
30900531	2	81	theme	tendon	362:367	arg1	lesions					369:375	equine tendon lesions	355:375	equine tendon lesions	355:375	Their secreted vesicles are important effectors in a wide array of biological processes, and contribute to in vivo healing of equine tendon lesions and endometrial inflammation.
30087563	1	0	from	component	227:235	arg1	modality					151:158	a safe, noninvasive modality	131:158	a safe, noninvasive modality	131:158	BACKGROUND Photodynamic therapy is a safe, noninvasive modality for cancer therapy, in which the photosensitizer (PS) is a crucial component.
30087563	1	0	from	component	227:235	arg1	therapy					120:126	BACKGROUND Photodynamic therapy	96:126	BACKGROUND Photodynamic therapy	96:126	BACKGROUND Photodynamic therapy is a safe, noninvasive modality for cancer therapy, in which the photosensitizer (PS) is a crucial component.
30087563	4	1	from	amount	657:662	arg1	nanoparticles					708:720	the Hy-entrapped PDA-MNP composite nanoparticles	673:720	the Hy-entrapped PDA-MNP composite nanoparticles	673:720	The amount of Hy in the Hy-entrapped PDA-MNP composite nanoparticles (denoted as PHMs) was measured by spectrophotometry.
30087563	9	2	theme	cytotoxicity	1502:1513	arg1	study					1515:1519	cytotoxicity study	1502:1519	cytotoxicity study	1502:1519	The results of intracellular reactive oxygen species generation detection, cytotoxicity study, and apoptosis detection indicated that the Lac-PHMs had a satisfying photodynamic effect to HepG2 cells.
30087563	10	3	theme	great	1681:1685	arg1	potential					1687:1695	great potential	1681:1695	great potential for delivery of a variety of hydrophobic PSs	1681:1740	CONCLUSION The strategy developed in this work offers great potential for delivery of a variety of hydrophobic PSs.
30087563	7	4	theme	asialoglycoprotein	1199:1216	arg1	receptors					1218:1226	asialoglycoprotein receptors	1199:1226	asialoglycoprotein receptors overexpressing HepG2 cells	1199:1253	The fabricated Hy-entrapped glyconanoparticle (Lac-PHM) exhibited excellent water dispersibility, stability, and selectivity for asialoglycoprotein receptors overexpressing HepG2 cells.
30087563	5	5	theme	reaction	935:942	arg1	advantage					906:914	advantage	906:914	advantage of the spontaneous reaction of PDA with amino groups	906:967	Furthermore, lactose, as the targeting ligand to asialoglycoprotein receptors, was conjugated to the surface of the PHMs by taking advantage of the spontaneous reaction of PDA with amino groups.
30087563	6	6	theme	Spectrophotometry	978:994	arg1	analysis					996:1003	Spectrophotometry analysis	978:1003	Spectrophotometry analysis	978:1003	RESULTS Spectrophotometry analysis revealed that the amount of Hy in the PHMs was 72 μmol g-1 PHMs.
30087563	10	7	theme	variety	1715:1721	arg1	delivery					1701:1708	delivery	1701:1708	delivery of a variety of hydrophobic PSs	1701:1740	CONCLUSION The strategy developed in this work offers great potential for delivery of a variety of hydrophobic PSs.
30087563	6	8	from	amount	1023:1028	arg1	PHMs					1043:1046	the PHMs	1039:1046	the PHMs	1039:1046	RESULTS Spectrophotometry analysis revealed that the amount of Hy in the PHMs was 72 μmol g-1 PHMs.
30087563	8	9	theme	Lac-PHMs	1326:1333	arg1	higher					1369:1374	higher	1369:1374	higher	1369:1374	Atomic absorption spectroscopy analysis showed that the amount of the Lac-PHMs taken in HepG2 cells was 2.1-fold higher than that of the triethylene glycol-modified PHMs.
30087563	8	9	theme	Lac-PHMs	1326:1333	arg1	amount					1312:1317	the amount	1308:1317	the amount of the Lac-PHMs taken in HepG2 cells	1308:1354	Atomic absorption spectroscopy analysis showed that the amount of the Lac-PHMs taken in HepG2 cells was 2.1-fold higher than that of the triethylene glycol-modified PHMs.
30087563	8	9	theme	Lac-PHMs	1326:1333	arg1	Lac-PHMs					1326:1333	the Lac-PHMs	1322:1333	the Lac-PHMs taken in HepG2 cells	1322:1354	Atomic absorption spectroscopy analysis showed that the amount of the Lac-PHMs taken in HepG2 cells was 2.1-fold higher than that of the triethylene glycol-modified PHMs.
30087563	9	10	theme	satisfying	1580:1589	arg1	effect					1604:1609	a satisfying photodynamic effect	1578:1609	a satisfying photodynamic effect	1578:1609	The results of intracellular reactive oxygen species generation detection, cytotoxicity study, and apoptosis detection indicated that the Lac-PHMs had a satisfying photodynamic effect to HepG2 cells.
30087563	4	11	theme	composite	698:706	arg1	nanoparticles					708:720	the Hy-entrapped PDA-MNP composite nanoparticles	673:720	the Hy-entrapped PDA-MNP composite nanoparticles	673:720	The amount of Hy in the Hy-entrapped PDA-MNP composite nanoparticles (denoted as PHMs) was measured by spectrophotometry.
30087563	9	12	contain	had	1574:1576	arg2	effect					1604:1609	a satisfying photodynamic effect	1578:1609	a satisfying photodynamic effect	1578:1609	The results of intracellular reactive oxygen species generation detection, cytotoxicity study, and apoptosis detection indicated that the Lac-PHMs had a satisfying photodynamic effect to HepG2 cells.
30087563	9	12	contain	had	1574:1576	arg1	Lac-PHMs					1565:1572	the Lac-PHMs	1561:1572	the Lac-PHMs	1561:1572	The results of intracellular reactive oxygen species generation detection, cytotoxicity study, and apoptosis detection indicated that the Lac-PHMs had a satisfying photodynamic effect to HepG2 cells.
30087563	5	13	gly	asialoglycoprotein	824:841	arg1	asialoglycoprotein					824:841	asialoglycoprotein receptors	824:851	asialoglycoprotein receptors	824:851	Furthermore, lactose, as the targeting ligand to asialoglycoprotein receptors, was conjugated to the surface of the PHMs by taking advantage of the spontaneous reaction of PDA with amino groups.
30087563	9	14	theme	intracellular	1442:1454	arg1	generation					1480:1489	intracellular reactive oxygen species generation	1442:1489	intracellular reactive oxygen species generation detection	1442:1499	The results of intracellular reactive oxygen species generation detection, cytotoxicity study, and apoptosis detection indicated that the Lac-PHMs had a satisfying photodynamic effect to HepG2 cells.
30087563	6	15	theme	μmol	1055:1058	arg1	Hy					1033:1034	Hy	1033:1034	Hy	1033:1034	RESULTS Spectrophotometry analysis revealed that the amount of Hy in the PHMs was 72 μmol g-1 PHMs.
30087563	6	15	theme	μmol	1055:1058	arg1	PHMs					1064:1067	72 μmol g-1 PHMs	1052:1067	72 μmol g-1 PHMs	1052:1067	RESULTS Spectrophotometry analysis revealed that the amount of Hy in the PHMs was 72 μmol g-1 PHMs.
30087563	6	15	theme	μmol	1055:1058	arg1	amount					1023:1028	the amount	1019:1028	the amount of Hy in the PHMs	1019:1046	RESULTS Spectrophotometry analysis revealed that the amount of Hy in the PHMs was 72 μmol g-1 PHMs.
30087563	7	16	theme	excellent	1136:1144	arg1	dispersibility					1152:1165	excellent water dispersibility	1136:1165	excellent water dispersibility	1136:1165	The fabricated Hy-entrapped glyconanoparticle (Lac-PHM) exhibited excellent water dispersibility, stability, and selectivity for asialoglycoprotein receptors overexpressing HepG2 cells.
30087563	9	17	theme	generation	1480:1489	arg1	detection					1491:1499	intracellular reactive oxygen species generation detection	1442:1499	intracellular reactive oxygen species generation detection	1442:1499	The results of intracellular reactive oxygen species generation detection, cytotoxicity study, and apoptosis detection indicated that the Lac-PHMs had a satisfying photodynamic effect to HepG2 cells.
30087563	5	18	with	reaction	935:942	arg1	groups					962:967	amino groups	956:967	amino groups	956:967	Furthermore, lactose, as the targeting ligand to asialoglycoprotein receptors, was conjugated to the surface of the PHMs by taking advantage of the spontaneous reaction of PDA with amino groups.
30087563	9	19	theme	reactive	1456:1463	arg1	species					1472:1478	reactive oxygen species	1456:1478	intracellular reactive oxygen species generation detection	1442:1499	The results of intracellular reactive oxygen species generation detection, cytotoxicity study, and apoptosis detection indicated that the Lac-PHMs had a satisfying photodynamic effect to HepG2 cells.
30087563	1	20	theme	BACKGROUND	96:105	arg1	modality					151:158	a safe, noninvasive modality	131:158	a safe, noninvasive modality	131:158	BACKGROUND Photodynamic therapy is a safe, noninvasive modality for cancer therapy, in which the photosensitizer (PS) is a crucial component.
30087563	1	20	theme	BACKGROUND	96:105	arg1	therapy					120:126	BACKGROUND Photodynamic therapy	96:126	BACKGROUND Photodynamic therapy	96:126	BACKGROUND Photodynamic therapy is a safe, noninvasive modality for cancer therapy, in which the photosensitizer (PS) is a crucial component.
30087563	5	21	theme	asialoglycoprotein	824:841	arg1	receptors					843:851	asialoglycoprotein receptors	824:851	asialoglycoprotein receptors	824:851	Furthermore, lactose, as the targeting ligand to asialoglycoprotein receptors, was conjugated to the surface of the PHMs by taking advantage of the spontaneous reaction of PDA with amino groups.
30087563	7	22	theme	water	1146:1150	arg1	dispersibility					1152:1165	excellent water dispersibility	1136:1165	excellent water dispersibility	1136:1165	The fabricated Hy-entrapped glyconanoparticle (Lac-PHM) exhibited excellent water dispersibility, stability, and selectivity for asialoglycoprotein receptors overexpressing HepG2 cells.
30087563	1	23	theme	Photodynamic	107:118	arg1	modality					151:158	a safe, noninvasive modality	131:158	a safe, noninvasive modality	131:158	BACKGROUND Photodynamic therapy is a safe, noninvasive modality for cancer therapy, in which the photosensitizer (PS) is a crucial component.
30087563	1	23	theme	Photodynamic	107:118	arg1	therapy					120:126	BACKGROUND Photodynamic therapy	96:126	BACKGROUND Photodynamic therapy	96:126	BACKGROUND Photodynamic therapy is a safe, noninvasive modality for cancer therapy, in which the photosensitizer (PS) is a crucial component.
30087563	3	24	theme	Hy	432:433	arg1	limitation					418:427	the clinical application limitation	393:427	the clinical application limitation of Hy	393:433	MATERIALS AND METHODS To overcome the clinical application limitation of Hy, a novel strategy is developed here by entrapping Hy into polydopamine (PDA) film formed on the surface of magnetic iron oxide nanoparticles (MNPs) through the self-polymerization of dopamine under alkaline condition.
30087563	3	25	theme	polydopamine	493:504	arg1	film					512:515	polydopamine (PDA) film	493:515	polydopamine (PDA) film formed on the surface of magnetic iron oxide nanoparticles (MNPs) through the self-polymerization of dopamine under alkaline condition	493:650	MATERIALS AND METHODS To overcome the clinical application limitation of Hy, a novel strategy is developed here by entrapping Hy into polydopamine (PDA) film formed on the surface of magnetic iron oxide nanoparticles (MNPs) through the self-polymerization of dopamine under alkaline condition.
30087563	10	26	theme	PSs	1738:1740	arg1	variety					1715:1721	a variety	1713:1721	a variety of hydrophobic PSs	1713:1740	CONCLUSION The strategy developed in this work offers great potential for delivery of a variety of hydrophobic PSs.
30087563	10	26	theme	PSs	1738:1740	arg1	PSs					1738:1740	hydrophobic PSs	1726:1740	hydrophobic PSs	1726:1740	CONCLUSION The strategy developed in this work offers great potential for delivery of a variety of hydrophobic PSs.
30087563	4	27	from	nanoparticles	708:720	arg1	amount					657:662	The amount	653:662	The amount of Hy in the Hy-entrapped PDA-MNP composite nanoparticles (denoted as PHMs)	653:738	The amount of Hy in the Hy-entrapped PDA-MNP composite nanoparticles (denoted as PHMs) was measured by spectrophotometry.
30087563	4	27	from	nanoparticles	708:720	arg1	Hy					667:668	Hy	667:668	Hy in the Hy-entrapped PDA-MNP composite nanoparticles	667:720	The amount of Hy in the Hy-entrapped PDA-MNP composite nanoparticles (denoted as PHMs) was measured by spectrophotometry.
30087563	2	28	theme	clinical	285:292	arg1	application					294:304	its clinical application	281:304	its clinical application	281:304	Hypericin (Hy) is a promising PS; however, its clinical application is significantly limited by its poor hydrophilicity.
30087563	3	29	theme	application	406:416	arg1	limitation					418:427	the clinical application limitation	393:427	the clinical application limitation of Hy	393:433	MATERIALS AND METHODS To overcome the clinical application limitation of Hy, a novel strategy is developed here by entrapping Hy into polydopamine (PDA) film formed on the surface of magnetic iron oxide nanoparticles (MNPs) through the self-polymerization of dopamine under alkaline condition.
30087563	0	30	theme	Facile	0:5	arg1	fabrication					7:17	Facile fabrication	0:17	Facile fabrication of hypericin-entrapped glyconanoparticles for targeted photodynamic therapy	0:93	Facile fabrication of hypericin-entrapped glyconanoparticles for targeted photodynamic therapy.
30087563	10	31	dep	CONCLUSION	1627:1636	arg1	offers					1674:1679	offers	1674:1679	offers great potential for delivery of a variety of hydrophobic PSs	1674:1740	CONCLUSION The strategy developed in this work offers great potential for delivery of a variety of hydrophobic PSs.
30087563	4	32	theme	PDA-MNP	690:696	arg1	nanoparticles					708:720	the Hy-entrapped PDA-MNP composite nanoparticles	673:720	the Hy-entrapped PDA-MNP composite nanoparticles	673:720	The amount of Hy in the Hy-entrapped PDA-MNP composite nanoparticles (denoted as PHMs) was measured by spectrophotometry.
30087563	0	33	theme	hypericin-entrapped	22:40	arg1	glyconanoparticles					42:59	hypericin-entrapped glyconanoparticles	22:59	hypericin-entrapped glyconanoparticles	22:59	Facile fabrication of hypericin-entrapped glyconanoparticles for targeted photodynamic therapy.
30087563	6	34	theme	Hy	1033:1034	arg1	PHMs					1064:1067	72 μmol g-1 PHMs	1052:1067	72 μmol g-1 PHMs	1052:1067	RESULTS Spectrophotometry analysis revealed that the amount of Hy in the PHMs was 72 μmol g-1 PHMs.
30087563	6	34	theme	Hy	1033:1034	arg1	Hy					1033:1034	Hy	1033:1034	Hy	1033:1034	RESULTS Spectrophotometry analysis revealed that the amount of Hy in the PHMs was 72 μmol g-1 PHMs.
30087563	6	34	theme	Hy	1033:1034	arg1	amount					1023:1028	the amount	1019:1028	the amount of Hy in the PHMs	1019:1046	RESULTS Spectrophotometry analysis revealed that the amount of Hy in the PHMs was 72 μmol g-1 PHMs.
30087563	9	35	theme	HepG2	1614:1618	arg1	cells					1620:1624	HepG2 cells	1614:1624	HepG2 cells	1614:1624	The results of intracellular reactive oxygen species generation detection, cytotoxicity study, and apoptosis detection indicated that the Lac-PHMs had a satisfying photodynamic effect to HepG2 cells.
30087563	9	36	theme	photodynamic	1591:1602	arg1	effect					1604:1609	a satisfying photodynamic effect	1578:1609	a satisfying photodynamic effect	1578:1609	The results of intracellular reactive oxygen species generation detection, cytotoxicity study, and apoptosis detection indicated that the Lac-PHMs had a satisfying photodynamic effect to HepG2 cells.
30087563	3	37	theme	novel	438:442	arg1	strategy					444:451	a novel strategy	436:451	a novel strategy	436:451	MATERIALS AND METHODS To overcome the clinical application limitation of Hy, a novel strategy is developed here by entrapping Hy into polydopamine (PDA) film formed on the surface of magnetic iron oxide nanoparticles (MNPs) through the self-polymerization of dopamine under alkaline condition.
30087563	8	38	theme	HepG2	1344:1348	arg1	cells					1350:1354	HepG2 cells	1344:1354	HepG2 cells	1344:1354	Atomic absorption spectroscopy analysis showed that the amount of the Lac-PHMs taken in HepG2 cells was 2.1-fold higher than that of the triethylene glycol-modified PHMs.
30087563	2	39	theme	promising	258:266	arg1	PS					268:269	a promising PS	256:269	a promising PS	256:269	Hypericin (Hy) is a promising PS; however, its clinical application is significantly limited by its poor hydrophilicity.
30087563	2	39	theme	promising	258:266	arg1	Hypericin					238:246	Hypericin	238:246	Hypericin (Hy)	238:251	Hypericin (Hy) is a promising PS; however, its clinical application is significantly limited by its poor hydrophilicity.
30087563	1	40	theme	safe	133:136	arg1	modality					151:158	a safe, noninvasive modality	131:158	a safe, noninvasive modality	131:158	BACKGROUND Photodynamic therapy is a safe, noninvasive modality for cancer therapy, in which the photosensitizer (PS) is a crucial component.
30087563	1	40	theme	safe	133:136	arg1	therapy					120:126	BACKGROUND Photodynamic therapy	96:126	BACKGROUND Photodynamic therapy	96:126	BACKGROUND Photodynamic therapy is a safe, noninvasive modality for cancer therapy, in which the photosensitizer (PS) is a crucial component.
30087563	9	41	theme	apoptosis	1526:1534	arg1	detection					1536:1544	apoptosis detection	1526:1544	apoptosis detection	1526:1544	The results of intracellular reactive oxygen species generation detection, cytotoxicity study, and apoptosis detection indicated that the Lac-PHMs had a satisfying photodynamic effect to HepG2 cells.
30087563	6	42	dep	RESULTS	970:976	arg1	revealed					1005:1012	revealed	1005:1012	revealed that the amount of Hy in the PHMs was 72 μmol g-1 PHMs	1005:1067	RESULTS Spectrophotometry analysis revealed that the amount of Hy in the PHMs was 72 μmol g-1 PHMs.
30087563	7	43	theme	Hy-entrapped	1085:1096	arg1	Lac-PHM					1117:1123	Lac-PHM	1117:1123	Lac-PHM	1117:1123	The fabricated Hy-entrapped glyconanoparticle (Lac-PHM) exhibited excellent water dispersibility, stability, and selectivity for asialoglycoprotein receptors overexpressing HepG2 cells.
30087563	7	43	theme	Hy-entrapped	1085:1096	arg1	glyconanoparticle					1098:1114	The fabricated Hy-entrapped glyconanoparticle	1070:1114	The fabricated Hy-entrapped glyconanoparticle (Lac-PHM)	1070:1124	The fabricated Hy-entrapped glyconanoparticle (Lac-PHM) exhibited excellent water dispersibility, stability, and selectivity for asialoglycoprotein receptors overexpressing HepG2 cells.
30087563	8	44	theme	absorption	1263:1272	arg1	spectroscopy					1274:1285	Atomic absorption spectroscopy	1256:1285	Atomic absorption spectroscopy analysis	1256:1294	Atomic absorption spectroscopy analysis showed that the amount of the Lac-PHMs taken in HepG2 cells was 2.1-fold higher than that of the triethylene glycol-modified PHMs.
30087563	7	45	gly	asialoglycoprotein	1199:1216	arg1	asialoglycoprotein					1199:1216	asialoglycoprotein receptors	1199:1226	asialoglycoprotein receptors overexpressing HepG2 cells	1199:1253	The fabricated Hy-entrapped glyconanoparticle (Lac-PHM) exhibited excellent water dispersibility, stability, and selectivity for asialoglycoprotein receptors overexpressing HepG2 cells.
30087563	9	46	theme	oxygen	1465:1470	arg1	species					1472:1478	reactive oxygen species	1456:1478	intracellular reactive oxygen species generation detection	1442:1499	The results of intracellular reactive oxygen species generation detection, cytotoxicity study, and apoptosis detection indicated that the Lac-PHMs had a satisfying photodynamic effect to HepG2 cells.
30087563	3	47	theme	iron	551:554	arg1	MNPs					577:580	MNPs	577:580	MNPs	577:580	MATERIALS AND METHODS To overcome the clinical application limitation of Hy, a novel strategy is developed here by entrapping Hy into polydopamine (PDA) film formed on the surface of magnetic iron oxide nanoparticles (MNPs) through the self-polymerization of dopamine under alkaline condition.
30087563	3	47	theme	iron	551:554	arg1	nanoparticles					562:574	magnetic iron oxide nanoparticles	542:574	magnetic iron oxide nanoparticles (MNPs)	542:581	MATERIALS AND METHODS To overcome the clinical application limitation of Hy, a novel strategy is developed here by entrapping Hy into polydopamine (PDA) film formed on the surface of magnetic iron oxide nanoparticles (MNPs) through the self-polymerization of dopamine under alkaline condition.
30087563	8	48	theme	Atomic	1256:1261	arg1	spectroscopy					1274:1285	Atomic absorption spectroscopy	1256:1285	Atomic absorption spectroscopy analysis	1256:1294	Atomic absorption spectroscopy analysis showed that the amount of the Lac-PHMs taken in HepG2 cells was 2.1-fold higher than that of the triethylene glycol-modified PHMs.
30087563	0	49	theme	glyconanoparticles	42:59	arg1	fabrication					7:17	Facile fabrication	0:17	Facile fabrication of hypericin-entrapped glyconanoparticles for targeted photodynamic therapy	0:93	Facile fabrication of hypericin-entrapped glyconanoparticles for targeted photodynamic therapy.
30087563	3	50	theme	clinical	397:404	arg1	limitation					418:427	the clinical application limitation	393:427	the clinical application limitation of Hy	393:433	MATERIALS AND METHODS To overcome the clinical application limitation of Hy, a novel strategy is developed here by entrapping Hy into polydopamine (PDA) film formed on the surface of magnetic iron oxide nanoparticles (MNPs) through the self-polymerization of dopamine under alkaline condition.
30087563	3	51	theme	oxide	556:560	arg1	MNPs					577:580	MNPs	577:580	MNPs	577:580	MATERIALS AND METHODS To overcome the clinical application limitation of Hy, a novel strategy is developed here by entrapping Hy into polydopamine (PDA) film formed on the surface of magnetic iron oxide nanoparticles (MNPs) through the self-polymerization of dopamine under alkaline condition.
30087563	3	51	theme	oxide	556:560	arg1	nanoparticles					562:574	magnetic iron oxide nanoparticles	542:574	magnetic iron oxide nanoparticles (MNPs)	542:581	MATERIALS AND METHODS To overcome the clinical application limitation of Hy, a novel strategy is developed here by entrapping Hy into polydopamine (PDA) film formed on the surface of magnetic iron oxide nanoparticles (MNPs) through the self-polymerization of dopamine under alkaline condition.
30087563	5	52	theme	targeting	804:812	arg1	ligand					814:819	the targeting ligand	800:819	the targeting ligand to asialoglycoprotein receptors	800:851	Furthermore, lactose, as the targeting ligand to asialoglycoprotein receptors, was conjugated to the surface of the PHMs by taking advantage of the spontaneous reaction of PDA with amino groups.
30087563	0	53	theme	photodynamic	74:85	arg1	therapy					87:93	targeted photodynamic therapy	65:93	targeted photodynamic therapy	65:93	Facile fabrication of hypericin-entrapped glyconanoparticles for targeted photodynamic therapy.
30087563	10	54	theme	hydrophobic	1726:1736	arg1	PSs					1738:1740	hydrophobic PSs	1726:1740	hydrophobic PSs	1726:1740	CONCLUSION The strategy developed in this work offers great potential for delivery of a variety of hydrophobic PSs.
30087563	9	55	theme	detection	1491:1499	arg1	results					1431:1437	The results	1427:1437	The results of intracellular reactive oxygen species generation detection, cytotoxicity study, and apoptosis detection	1427:1544	The results of intracellular reactive oxygen species generation detection, cytotoxicity study, and apoptosis detection indicated that the Lac-PHMs had a satisfying photodynamic effect to HepG2 cells.
30087563	8	56	theme	spectroscopy	1274:1285	arg1	analysis					1287:1294	Atomic absorption spectroscopy analysis	1256:1294	Atomic absorption spectroscopy analysis	1256:1294	Atomic absorption spectroscopy analysis showed that the amount of the Lac-PHMs taken in HepG2 cells was 2.1-fold higher than that of the triethylene glycol-modified PHMs.
30087563	0	57	theme	targeted	65:72	arg1	therapy					87:93	targeted photodynamic therapy	65:93	targeted photodynamic therapy	65:93	Facile fabrication of hypericin-entrapped glyconanoparticles for targeted photodynamic therapy.
30087563	3	58	theme	alkaline	633:640	arg1	condition					642:650	alkaline condition	633:650	alkaline condition	633:650	MATERIALS AND METHODS To overcome the clinical application limitation of Hy, a novel strategy is developed here by entrapping Hy into polydopamine (PDA) film formed on the surface of magnetic iron oxide nanoparticles (MNPs) through the self-polymerization of dopamine under alkaline condition.
30087563	3	59	theme	magnetic	542:549	arg1	MNPs					577:580	MNPs	577:580	MNPs	577:580	MATERIALS AND METHODS To overcome the clinical application limitation of Hy, a novel strategy is developed here by entrapping Hy into polydopamine (PDA) film formed on the surface of magnetic iron oxide nanoparticles (MNPs) through the self-polymerization of dopamine under alkaline condition.
30087563	3	59	theme	magnetic	542:549	arg1	nanoparticles					562:574	magnetic iron oxide nanoparticles	542:574	magnetic iron oxide nanoparticles (MNPs)	542:581	MATERIALS AND METHODS To overcome the clinical application limitation of Hy, a novel strategy is developed here by entrapping Hy into polydopamine (PDA) film formed on the surface of magnetic iron oxide nanoparticles (MNPs) through the self-polymerization of dopamine under alkaline condition.
30087563	4	60	theme	Hy	667:668	arg1	amount					657:662	The amount	653:662	The amount of Hy in the Hy-entrapped PDA-MNP composite nanoparticles (denoted as PHMs)	653:738	The amount of Hy in the Hy-entrapped PDA-MNP composite nanoparticles (denoted as PHMs) was measured by spectrophotometry.
30087563	4	60	theme	Hy	667:668	arg1	Hy					667:668	Hy	667:668	Hy in the Hy-entrapped PDA-MNP composite nanoparticles	667:720	The amount of Hy in the Hy-entrapped PDA-MNP composite nanoparticles (denoted as PHMs) was measured by spectrophotometry.
30087563	8	61	theme	glycol-modified	1405:1419	arg1	PHMs					1421:1424	the triethylene glycol-modified PHMs	1389:1424	the triethylene glycol-modified PHMs	1389:1424	Atomic absorption spectroscopy analysis showed that the amount of the Lac-PHMs taken in HepG2 cells was 2.1-fold higher than that of the triethylene glycol-modified PHMs.
30087563	5	62	theme	PHMs	891:894	arg1	surface					876:882	the surface	872:882	the surface of the PHMs	872:894	Furthermore, lactose, as the targeting ligand to asialoglycoprotein receptors, was conjugated to the surface of the PHMs by taking advantage of the spontaneous reaction of PDA with amino groups.
30087563	8	63	theme	triethylene	1393:1403	arg1	PHMs					1421:1424	the triethylene glycol-modified PHMs	1389:1424	the triethylene glycol-modified PHMs	1389:1424	Atomic absorption spectroscopy analysis showed that the amount of the Lac-PHMs taken in HepG2 cells was 2.1-fold higher than that of the triethylene glycol-modified PHMs.
30087563	7	64	theme	fabricated	1074:1083	arg1	Lac-PHM					1117:1123	Lac-PHM	1117:1123	Lac-PHM	1117:1123	The fabricated Hy-entrapped glyconanoparticle (Lac-PHM) exhibited excellent water dispersibility, stability, and selectivity for asialoglycoprotein receptors overexpressing HepG2 cells.
30087563	7	64	theme	fabricated	1074:1083	arg1	glyconanoparticle					1098:1114	The fabricated Hy-entrapped glyconanoparticle	1070:1114	The fabricated Hy-entrapped glyconanoparticle (Lac-PHM)	1070:1124	The fabricated Hy-entrapped glyconanoparticle (Lac-PHM) exhibited excellent water dispersibility, stability, and selectivity for asialoglycoprotein receptors overexpressing HepG2 cells.
30087563	4	65	theme	Hy-entrapped	677:688	arg1	nanoparticles					708:720	the Hy-entrapped PDA-MNP composite nanoparticles	673:720	the Hy-entrapped PDA-MNP composite nanoparticles	673:720	The amount of Hy in the Hy-entrapped PDA-MNP composite nanoparticles (denoted as PHMs) was measured by spectrophotometry.
30087563	9	66	theme	detection	1536:1544	arg1	results					1431:1437	The results	1427:1437	The results of intracellular reactive oxygen species generation detection, cytotoxicity study, and apoptosis detection	1427:1544	The results of intracellular reactive oxygen species generation detection, cytotoxicity study, and apoptosis detection indicated that the Lac-PHMs had a satisfying photodynamic effect to HepG2 cells.
30087563	1	67	theme	crucial	219:225	arg1	photosensitizer					193:207	the photosensitizer	189:207	the photosensitizer (PS)	189:212	BACKGROUND Photodynamic therapy is a safe, noninvasive modality for cancer therapy, in which the photosensitizer (PS) is a crucial component.
30087563	1	67	theme	crucial	219:225	arg1	component					227:235	a crucial component	217:235	a crucial component	217:235	BACKGROUND Photodynamic therapy is a safe, noninvasive modality for cancer therapy, in which the photosensitizer (PS) is a crucial component.
30087563	3	68	theme	PDA	507:509	arg1	film					512:515	polydopamine (PDA) film	493:515	polydopamine (PDA) film formed on the surface of magnetic iron oxide nanoparticles (MNPs) through the self-polymerization of dopamine under alkaline condition	493:650	MATERIALS AND METHODS To overcome the clinical application limitation of Hy, a novel strategy is developed here by entrapping Hy into polydopamine (PDA) film formed on the surface of magnetic iron oxide nanoparticles (MNPs) through the self-polymerization of dopamine under alkaline condition.
30087563	3	69	theme	dopamine	618:625	arg1	self-polymerization					595:613	the self-polymerization	591:613	the self-polymerization of dopamine under alkaline condition	591:650	MATERIALS AND METHODS To overcome the clinical application limitation of Hy, a novel strategy is developed here by entrapping Hy into polydopamine (PDA) film formed on the surface of magnetic iron oxide nanoparticles (MNPs) through the self-polymerization of dopamine under alkaline condition.
30087563	5	70	theme	PDA	947:949	arg1	reaction					935:942	the spontaneous reaction	919:942	the spontaneous reaction of PDA with amino groups	919:967	Furthermore, lactose, as the targeting ligand to asialoglycoprotein receptors, was conjugated to the surface of the PHMs by taking advantage of the spontaneous reaction of PDA with amino groups.
30087563	1	71	dep	safe	133:136	arg1	noninvasive					139:149	noninvasive	139:149	noninvasive	139:149	BACKGROUND Photodynamic therapy is a safe, noninvasive modality for cancer therapy, in which the photosensitizer (PS) is a crucial component.
30087563	6	72	theme	g-1	1060:1062	arg1	Hy					1033:1034	Hy	1033:1034	Hy	1033:1034	RESULTS Spectrophotometry analysis revealed that the amount of Hy in the PHMs was 72 μmol g-1 PHMs.
30087563	6	72	theme	g-1	1060:1062	arg1	PHMs					1064:1067	72 μmol g-1 PHMs	1052:1067	72 μmol g-1 PHMs	1052:1067	RESULTS Spectrophotometry analysis revealed that the amount of Hy in the PHMs was 72 μmol g-1 PHMs.
30087563	6	72	theme	g-1	1060:1062	arg1	amount					1023:1028	the amount	1019:1028	the amount of Hy in the PHMs	1019:1046	RESULTS Spectrophotometry analysis revealed that the amount of Hy in the PHMs was 72 μmol g-1 PHMs.
30087563	9	73	theme	species	1472:1478	arg1	generation					1480:1489	intracellular reactive oxygen species generation	1442:1489	intracellular reactive oxygen species generation detection	1442:1499	The results of intracellular reactive oxygen species generation detection, cytotoxicity study, and apoptosis detection indicated that the Lac-PHMs had a satisfying photodynamic effect to HepG2 cells.
30087563	5	74	theme	spontaneous	923:933	arg1	reaction					935:942	the spontaneous reaction	919:942	the spontaneous reaction of PDA with amino groups	919:967	Furthermore, lactose, as the targeting ligand to asialoglycoprotein receptors, was conjugated to the surface of the PHMs by taking advantage of the spontaneous reaction of PDA with amino groups.
30087563	3	75	theme	nanoparticles	562:574	arg1	surface					531:537	the surface	527:537	the surface of magnetic iron oxide nanoparticles (MNPs)	527:581	MATERIALS AND METHODS To overcome the clinical application limitation of Hy, a novel strategy is developed here by entrapping Hy into polydopamine (PDA) film formed on the surface of magnetic iron oxide nanoparticles (MNPs) through the self-polymerization of dopamine under alkaline condition.
30087563	9	76	theme	study	1515:1519	arg1	results					1431:1437	The results	1427:1437	The results of intracellular reactive oxygen species generation detection, cytotoxicity study, and apoptosis detection	1427:1544	The results of intracellular reactive oxygen species generation detection, cytotoxicity study, and apoptosis detection indicated that the Lac-PHMs had a satisfying photodynamic effect to HepG2 cells.
30087563	1	77	theme	cancer	164:169	arg1	therapy					171:177	cancer therapy	164:177	cancer therapy	164:177	BACKGROUND Photodynamic therapy is a safe, noninvasive modality for cancer therapy, in which the photosensitizer (PS) is a crucial component.
30087563	4	78	from	Hy	667:668	arg1	nanoparticles					708:720	the Hy-entrapped PDA-MNP composite nanoparticles	673:720	the Hy-entrapped PDA-MNP composite nanoparticles	673:720	The amount of Hy in the Hy-entrapped PDA-MNP composite nanoparticles (denoted as PHMs) was measured by spectrophotometry.
30087563	5	79	theme	amino	956:960	arg1	groups					962:967	amino groups	956:967	amino groups	956:967	Furthermore, lactose, as the targeting ligand to asialoglycoprotein receptors, was conjugated to the surface of the PHMs by taking advantage of the spontaneous reaction of PDA with amino groups.
30087563	7	80	theme	HepG2	1243:1247	arg1	cells					1249:1253	HepG2 cells	1243:1253	HepG2 cells	1243:1253	The fabricated Hy-entrapped glyconanoparticle (Lac-PHM) exhibited excellent water dispersibility, stability, and selectivity for asialoglycoprotein receptors overexpressing HepG2 cells.
30087563	2	81	theme	poor	338:341	arg1	hydrophilicity					343:356	its poor hydrophilicity	334:356	its poor hydrophilicity	334:356	Hypericin (Hy) is a promising PS; however, its clinical application is significantly limited by its poor hydrophilicity.
30532218	2	0	dep	approach	578:585	arg1	employed					688:695	employed	688:695	was employed to optimize the production of monascus pigments using co-solid state fermentation of the two raw agro-industrial wastes	684:815	A three step sequential, statistical modeling approach: one variable at a time (OVAT), Plackett-Burman design (PBD), and central composite design (CCD) was employed to optimize the production of monascus pigments using co-solid state fermentation of the two raw agro-industrial wastes.
30532218	0	1	theme	cost	172:175	arg1	approach					177:184	An eco-friendly environmental low cost approach	138:184	Monascus orange and red pigments production by Monascus purpureus ATCC16436 through co-solid state fermentation of corn cob and glycerol: An eco-friendly environmental low cost approach.	0:185	Monascus orange and red pigments production by Monascus purpureus ATCC16436 through co-solid state fermentation of corn cob and glycerol: An eco-friendly environmental low cost approach.
30532218	5	2	theme	value	1306:1310	arg1	units/mL					1312:1319	133.77 and 108.02 color value units/mL	1282:1319	133.77 and 108.02 color value units/mL	1282:1319	The optimal levels of inoculum size (12 x 1011 spores/mL) and glycerol (2.17 M) did achieve a maximal color value of 133.77 and 108.02 color value units/mL of orange and red pigments, respectively at 30 oC after 10 days; concluded from CCD with an agitation speed of 150 rpm.
30532218	3	3	theme	triggering	945:954	arg1	cob					823:825	Corn cob	818:825	Corn cob among other carbon sources (e.g., rice grains, sugarcane bagasse, and potato peel)	818:908	Corn cob among other carbon sources (e.g., rice grains, sugarcane bagasse, and potato peel) was the most appropriate substrate triggering co-production of orange and red monascus pigments; deduced from OVAT.
30532218	3	3	theme	triggering	945:954	arg1	co-production					956:968	the most appropriate substrate triggering co-production	914:968	the most appropriate substrate triggering co-production of orange and red monascus pigments	914:1004	Corn cob among other carbon sources (e.g., rice grains, sugarcane bagasse, and potato peel) was the most appropriate substrate triggering co-production of orange and red monascus pigments; deduced from OVAT.
30532218	2	4	dep	sequential	545:554	arg1	statistical					557:567	statistical	557:567	statistical	557:567	A three step sequential, statistical modeling approach: one variable at a time (OVAT), Plackett-Burman design (PBD), and central composite design (CCD) was employed to optimize the production of monascus pigments using co-solid state fermentation of the two raw agro-industrial wastes.
30532218	3	5	theme	appropriate	923:933	arg1	cob					823:825	Corn cob	818:825	Corn cob among other carbon sources (e.g., rice grains, sugarcane bagasse, and potato peel)	818:908	Corn cob among other carbon sources (e.g., rice grains, sugarcane bagasse, and potato peel) was the most appropriate substrate triggering co-production of orange and red monascus pigments; deduced from OVAT.
30532218	3	5	theme	appropriate	923:933	arg1	co-production					956:968	the most appropriate substrate triggering co-production	914:968	the most appropriate substrate triggering co-production of orange and red monascus pigments	914:1004	Corn cob among other carbon sources (e.g., rice grains, sugarcane bagasse, and potato peel) was the most appropriate substrate triggering co-production of orange and red monascus pigments; deduced from OVAT.
30532218	1	6	theme	red	467:469	arg1	pigments					471:478	red pigments	467:478	red pigments	467:478	The present study underlines a statistically optimized, low cost, effective approach for efficient co-valorization of two non-efficiently utilized, highly accumulated, raw agro-industrial wastes: corn cob and glycerol for co-production of natural biopigments: monascus orange and red pigments by the aid of Monascus purpureus strain ATCC 16436.
30532218	2	7	from	design	671:676	arg1	variable					592:599	one variable	588:599	one variable at a time (OVAT), Plackett-Burman design (PBD), and central composite design (CCD)	588:682	A three step sequential, statistical modeling approach: one variable at a time (OVAT), Plackett-Burman design (PBD), and central composite design (CCD) was employed to optimize the production of monascus pigments using co-solid state fermentation of the two raw agro-industrial wastes.
30532218	2	8	theme	sequential	545:554	arg1	approach					578:585	A three step sequential, statistical modeling approach	532:585	A three step sequential, statistical modeling approach: one variable at a time (OVAT), Plackett-Burman design (PBD), and central composite design (CCD) was employed to optimize the production of monascus pigments using co-solid state fermentation of the two raw agro-industrial wastes.	532:816	A three step sequential, statistical modeling approach: one variable at a time (OVAT), Plackett-Burman design (PBD), and central composite design (CCD) was employed to optimize the production of monascus pigments using co-solid state fermentation of the two raw agro-industrial wastes.
30532218	4	9	theme	Glycerol	1026:1033	arg1	size					1048:1051	Glycerol and inoculum size	1026:1051	size	1048:1051	Glycerol and inoculum size proved to impose significant consequences (P<0.05) on the production of monascus pigments as inferred from PBD.
30532218	0	10	theme	cob	120:122	arg1	fermentation					99:110	co-solid state fermentation	84:110	co-solid state fermentation of corn cob and glycerol	84:135	Monascus orange and red pigments production by Monascus purpureus ATCC16436 through co-solid state fermentation of corn cob and glycerol: An eco-friendly environmental low cost approach.
30532218	4	11	theme	inoculum	1039:1046	arg1	size					1048:1051	Glycerol and inoculum size	1026:1051	size	1048:1051	Glycerol and inoculum size proved to impose significant consequences (P<0.05) on the production of monascus pigments as inferred from PBD.
30532218	0	12	theme	glycerol	128:135	arg1	fermentation					99:110	co-solid state fermentation	84:110	co-solid state fermentation of corn cob and glycerol	84:135	Monascus orange and red pigments production by Monascus purpureus ATCC16436 through co-solid state fermentation of corn cob and glycerol: An eco-friendly environmental low cost approach.
30532218	3	13	dep	sources	846:852	arg1	grains					866:871	rice grains	861:871	rice grains	861:871	Corn cob among other carbon sources (e.g., rice grains, sugarcane bagasse, and potato peel) was the most appropriate substrate triggering co-production of orange and red monascus pigments; deduced from OVAT.
30532218	3	13	dep	sources	846:852	arg1	bagasse					884:890	sugarcane bagasse	874:890	sugarcane bagasse	874:890	Corn cob among other carbon sources (e.g., rice grains, sugarcane bagasse, and potato peel) was the most appropriate substrate triggering co-production of orange and red monascus pigments; deduced from OVAT.
30532218	3	13	dep	sources	846:852	arg1	peel					904:907	potato peel	897:907	potato peel	897:907	Corn cob among other carbon sources (e.g., rice grains, sugarcane bagasse, and potato peel) was the most appropriate substrate triggering co-production of orange and red monascus pigments; deduced from OVAT.
30532218	5	14	from	oC	1368:1369	arg1	units/mL					1312:1319	133.77 and 108.02 color value units/mL	1282:1319	133.77 and 108.02 color value units/mL	1282:1319	The optimal levels of inoculum size (12 x 1011 spores/mL) and glycerol (2.17 M) did achieve a maximal color value of 133.77 and 108.02 color value units/mL of orange and red pigments, respectively at 30 oC after 10 days; concluded from CCD with an agitation speed of 150 rpm.
30532218	5	14	from	oC	1368:1369	arg1	value					1273:1277	a maximal color value	1257:1277	a maximal color value of 133.77 and 108.02 color value units/mL of orange and red pigments, respectively at 30 oC after 10 days	1257:1383	The optimal levels of inoculum size (12 x 1011 spores/mL) and glycerol (2.17 M) did achieve a maximal color value of 133.77 and 108.02 color value units/mL of orange and red pigments, respectively at 30 oC after 10 days; concluded from CCD with an agitation speed of 150 rpm.
30532218	6	15	theme	present	1527:1533	arg1	approach					1535:1542	the present approach	1523:1542	the present approach for efficient exploitation of such biopigments in food, pharmaceutical and textile industries	1523:1636	Present data would underpin the large scale production of monascus pigments using the present approach for efficient exploitation of such biopigments in food, pharmaceutical and textile industries.
30532218	2	16	theme	composite	661:669	arg1	CCD					679:681	CCD	679:681	CCD	679:681	A three step sequential, statistical modeling approach: one variable at a time (OVAT), Plackett-Burman design (PBD), and central composite design (CCD) was employed to optimize the production of monascus pigments using co-solid state fermentation of the two raw agro-industrial wastes.
30532218	2	16	theme	composite	661:669	arg1	design					671:676	central composite design	653:676	central composite design (CCD)	653:682	A three step sequential, statistical modeling approach: one variable at a time (OVAT), Plackett-Burman design (PBD), and central composite design (CCD) was employed to optimize the production of monascus pigments using co-solid state fermentation of the two raw agro-industrial wastes.
30532218	1	17	dep	approach	263:270	arg1	glycerol					396:403	glycerol	396:403	glycerol	396:403	The present study underlines a statistically optimized, low cost, effective approach for efficient co-valorization of two non-efficiently utilized, highly accumulated, raw agro-industrial wastes: corn cob and glycerol for co-production of natural biopigments: monascus orange and red pigments by the aid of Monascus purpureus strain ATCC 16436.
30532218	1	17	dep	approach	263:270	arg1	orange					456:461	monascus orange	447:461	monascus orange	447:461	The present study underlines a statistically optimized, low cost, effective approach for efficient co-valorization of two non-efficiently utilized, highly accumulated, raw agro-industrial wastes: corn cob and glycerol for co-production of natural biopigments: monascus orange and red pigments by the aid of Monascus purpureus strain ATCC 16436.
30532218	1	17	dep	approach	263:270	arg1	cob					388:390	corn cob	383:390	corn cob	383:390	The present study underlines a statistically optimized, low cost, effective approach for efficient co-valorization of two non-efficiently utilized, highly accumulated, raw agro-industrial wastes: corn cob and glycerol for co-production of natural biopigments: monascus orange and red pigments by the aid of Monascus purpureus strain ATCC 16436.
30532218	1	17	dep	approach	263:270	arg1	pigments					471:478	red pigments	467:478	red pigments	467:478	The present study underlines a statistically optimized, low cost, effective approach for efficient co-valorization of two non-efficiently utilized, highly accumulated, raw agro-industrial wastes: corn cob and glycerol for co-production of natural biopigments: monascus orange and red pigments by the aid of Monascus purpureus strain ATCC 16436.
30532218	1	18	theme	monascus	447:454	arg1	orange					456:461	monascus orange	447:461	monascus orange	447:461	The present study underlines a statistically optimized, low cost, effective approach for efficient co-valorization of two non-efficiently utilized, highly accumulated, raw agro-industrial wastes: corn cob and glycerol for co-production of natural biopigments: monascus orange and red pigments by the aid of Monascus purpureus strain ATCC 16436.
30532218	2	19	from	design	635:640	arg1	variable					592:599	one variable	588:599	one variable at a time (OVAT), Plackett-Burman design (PBD), and central composite design (CCD)	588:682	A three step sequential, statistical modeling approach: one variable at a time (OVAT), Plackett-Burman design (PBD), and central composite design (CCD) was employed to optimize the production of monascus pigments using co-solid state fermentation of the two raw agro-industrial wastes.
30532218	1	20	theme	natural	426:432	arg1	biopigments					434:444	natural biopigments	426:444	natural biopigments	426:444	The present study underlines a statistically optimized, low cost, effective approach for efficient co-valorization of two non-efficiently utilized, highly accumulated, raw agro-industrial wastes: corn cob and glycerol for co-production of natural biopigments: monascus orange and red pigments by the aid of Monascus purpureus strain ATCC 16436.
30532218	6	21	theme	monascus	1499:1506	arg1	pigments					1508:1515	monascus pigments	1499:1515	monascus pigments using the present approach for efficient exploitation of such biopigments in food, pharmaceutical and textile industries	1499:1636	Present data would underpin the large scale production of monascus pigments using the present approach for efficient exploitation of such biopigments in food, pharmaceutical and textile industries.
30532218	5	22	theme	x	1205:1205	arg1	spores/mL					1212:1220	12 x 1011 spores/mL	1202:1220	12 x 1011 spores/mL	1202:1220	The optimal levels of inoculum size (12 x 1011 spores/mL) and glycerol (2.17 M) did achieve a maximal color value of 133.77 and 108.02 color value units/mL of orange and red pigments, respectively at 30 oC after 10 days; concluded from CCD with an agitation speed of 150 rpm.
30532218	5	22	theme	x	1205:1205	arg1	size					1196:1199	inoculum size	1187:1199	inoculum size (12 x 1011 spores/mL)	1187:1221	The optimal levels of inoculum size (12 x 1011 spores/mL) and glycerol (2.17 M) did achieve a maximal color value of 133.77 and 108.02 color value units/mL of orange and red pigments, respectively at 30 oC after 10 days; concluded from CCD with an agitation speed of 150 rpm.
30532218	3	23	theme	other	833:837	arg1	sources					846:852	other carbon sources	833:852	other carbon sources (e.g., rice grains, sugarcane bagasse, and potato peel)	833:908	Corn cob among other carbon sources (e.g., rice grains, sugarcane bagasse, and potato peel) was the most appropriate substrate triggering co-production of orange and red monascus pigments; deduced from OVAT.
30532218	2	24	theme	Plackett-Burman	619:633	arg1	PBD					643:645	PBD	643:645	PBD	643:645	A three step sequential, statistical modeling approach: one variable at a time (OVAT), Plackett-Burman design (PBD), and central composite design (CCD) was employed to optimize the production of monascus pigments using co-solid state fermentation of the two raw agro-industrial wastes.
30532218	2	24	theme	Plackett-Burman	619:633	arg1	design					635:640	Plackett-Burman design	619:640	Plackett-Burman design (PBD)	619:646	A three step sequential, statistical modeling approach: one variable at a time (OVAT), Plackett-Burman design (PBD), and central composite design (CCD) was employed to optimize the production of monascus pigments using co-solid state fermentation of the two raw agro-industrial wastes.
30532218	5	25	theme	inoculum	1187:1194	arg1	spores/mL					1212:1220	12 x 1011 spores/mL	1202:1220	12 x 1011 spores/mL	1202:1220	The optimal levels of inoculum size (12 x 1011 spores/mL) and glycerol (2.17 M) did achieve a maximal color value of 133.77 and 108.02 color value units/mL of orange and red pigments, respectively at 30 oC after 10 days; concluded from CCD with an agitation speed of 150 rpm.
30532218	5	25	theme	inoculum	1187:1194	arg1	size					1196:1199	inoculum size	1187:1199	inoculum size (12 x 1011 spores/mL)	1187:1221	The optimal levels of inoculum size (12 x 1011 spores/mL) and glycerol (2.17 M) did achieve a maximal color value of 133.77 and 108.02 color value units/mL of orange and red pigments, respectively at 30 oC after 10 days; concluded from CCD with an agitation speed of 150 rpm.
30532218	0	26	theme	state	93:97	arg1	fermentation					99:110	co-solid state fermentation	84:110	co-solid state fermentation of corn cob and glycerol	84:135	Monascus orange and red pigments production by Monascus purpureus ATCC16436 through co-solid state fermentation of corn cob and glycerol: An eco-friendly environmental low cost approach.
30532218	3	27	theme	monascus	988:995	arg1	pigments					997:1004	red monascus pigments	984:1004	red monascus pigments	984:1004	Corn cob among other carbon sources (e.g., rice grains, sugarcane bagasse, and potato peel) was the most appropriate substrate triggering co-production of orange and red monascus pigments; deduced from OVAT.
30532218	6	28	theme	scale	1479:1483	arg1	production					1485:1494	the large scale production	1469:1494	the large scale production of monascus pigments using the present approach for efficient exploitation of such biopigments in food, pharmaceutical and textile industries	1469:1636	Present data would underpin the large scale production of monascus pigments using the present approach for efficient exploitation of such biopigments in food, pharmaceutical and textile industries.
30532218	6	29	from	biopigments	1579:1589	arg1	industries					1627:1636	food, pharmaceutical and textile industries	1594:1636	food, pharmaceutical and textile industries	1594:1636	Present data would underpin the large scale production of monascus pigments using the present approach for efficient exploitation of such biopigments in food, pharmaceutical and textile industries.
30532218	6	30	theme	such	1574:1577	arg1	biopigments					1579:1589	such biopigments	1574:1589	such biopigments in food, pharmaceutical and textile industries	1574:1636	Present data would underpin the large scale production of monascus pigments using the present approach for efficient exploitation of such biopigments in food, pharmaceutical and textile industries.
30532218	5	31	theme	pigments	1339:1346	arg1	units/mL					1312:1319	133.77 and 108.02 color value units/mL	1282:1319	133.77 and 108.02 color value units/mL	1282:1319	The optimal levels of inoculum size (12 x 1011 spores/mL) and glycerol (2.17 M) did achieve a maximal color value of 133.77 and 108.02 color value units/mL of orange and red pigments, respectively at 30 oC after 10 days; concluded from CCD with an agitation speed of 150 rpm.
30532218	4	32	theme	monascus	1125:1132	arg1	pigments					1134:1141	monascus pigments	1125:1141	monascus pigments	1125:1141	Glycerol and inoculum size proved to impose significant consequences (P<0.05) on the production of monascus pigments as inferred from PBD.
30532218	0	33	theme	Monascus	0:7	arg1	production					33:42	Monascus orange and red pigments production	0:42	Monascus orange and red pigments production by Monascus purpureus ATCC16436 through co-solid state fermentation of corn cob and glycerol: An eco-friendly environmental low cost approach.	0:185	Monascus orange and red pigments production by Monascus purpureus ATCC16436 through co-solid state fermentation of corn cob and glycerol: An eco-friendly environmental low cost approach.
30532218	5	34	theme	orange	1324:1329	arg1	units/mL					1312:1319	133.77 and 108.02 color value units/mL	1282:1319	133.77 and 108.02 color value units/mL	1282:1319	The optimal levels of inoculum size (12 x 1011 spores/mL) and glycerol (2.17 M) did achieve a maximal color value of 133.77 and 108.02 color value units/mL of orange and red pigments, respectively at 30 oC after 10 days; concluded from CCD with an agitation speed of 150 rpm.
30532218	3	35	theme	rice	861:864	arg1	grains					866:871	rice grains	861:871	rice grains	861:871	Corn cob among other carbon sources (e.g., rice grains, sugarcane bagasse, and potato peel) was the most appropriate substrate triggering co-production of orange and red monascus pigments; deduced from OVAT.
30532218	0	36	theme	pigments	24:31	arg1	production					33:42	Monascus orange and red pigments production	0:42	Monascus orange and red pigments production by Monascus purpureus ATCC16436 through co-solid state fermentation of corn cob and glycerol: An eco-friendly environmental low cost approach.	0:185	Monascus orange and red pigments production by Monascus purpureus ATCC16436 through co-solid state fermentation of corn cob and glycerol: An eco-friendly environmental low cost approach.
30532218	5	37	theme	color	1267:1271	arg1	value					1273:1277	a maximal color value	1257:1277	a maximal color value of 133.77 and 108.02 color value units/mL of orange and red pigments, respectively at 30 oC after 10 days	1257:1383	The optimal levels of inoculum size (12 x 1011 spores/mL) and glycerol (2.17 M) did achieve a maximal color value of 133.77 and 108.02 color value units/mL of orange and red pigments, respectively at 30 oC after 10 days; concluded from CCD with an agitation speed of 150 rpm.
30532218	1	38	theme	utilized	325:332	arg1	wastes					375:380	two non-efficiently utilized, highly accumulated, raw agro-industrial wastes	305:380	two non-efficiently utilized, highly accumulated, raw agro-industrial wastes	305:380	The present study underlines a statistically optimized, low cost, effective approach for efficient co-valorization of two non-efficiently utilized, highly accumulated, raw agro-industrial wastes: corn cob and glycerol for co-production of natural biopigments: monascus orange and red pigments by the aid of Monascus purpureus strain ATCC 16436.
30532218	5	39	with	CCD	1401:1403	arg1	agitation					1413:1421	an agitation	1410:1421	an agitation speed of 150 rpm	1410:1438	The optimal levels of inoculum size (12 x 1011 spores/mL) and glycerol (2.17 M) did achieve a maximal color value of 133.77 and 108.02 color value units/mL of orange and red pigments, respectively at 30 oC after 10 days; concluded from CCD with an agitation speed of 150 rpm.
30532218	1	40	theme	purpureus	503:511	arg1	ATCC					520:523	Monascus purpureus strain ATCC 16436	494:529	Monascus purpureus strain ATCC 16436	494:529	The present study underlines a statistically optimized, low cost, effective approach for efficient co-valorization of two non-efficiently utilized, highly accumulated, raw agro-industrial wastes: corn cob and glycerol for co-production of natural biopigments: monascus orange and red pigments by the aid of Monascus purpureus strain ATCC 16436.
30532218	2	41	theme	co-solid	751:758	arg1	fermentation					766:777	co-solid state fermentation	751:777	co-solid state fermentation of the two raw agro-industrial wastes	751:815	A three step sequential, statistical modeling approach: one variable at a time (OVAT), Plackett-Burman design (PBD), and central composite design (CCD) was employed to optimize the production of monascus pigments using co-solid state fermentation of the two raw agro-industrial wastes.
30532218	2	42	theme	raw	790:792	arg1	wastes					810:815	the two raw agro-industrial wastes	782:815	the two raw agro-industrial wastes	782:815	A three step sequential, statistical modeling approach: one variable at a time (OVAT), Plackett-Burman design (PBD), and central composite design (CCD) was employed to optimize the production of monascus pigments using co-solid state fermentation of the two raw agro-industrial wastes.
30532218	2	43	theme	agro-industrial	794:808	arg1	wastes					810:815	the two raw agro-industrial wastes	782:815	the two raw agro-industrial wastes	782:815	A three step sequential, statistical modeling approach: one variable at a time (OVAT), Plackett-Burman design (PBD), and central composite design (CCD) was employed to optimize the production of monascus pigments using co-solid state fermentation of the two raw agro-industrial wastes.
30532218	3	44	theme	substrate	935:943	arg1	cob					823:825	Corn cob	818:825	Corn cob among other carbon sources (e.g., rice grains, sugarcane bagasse, and potato peel)	818:908	Corn cob among other carbon sources (e.g., rice grains, sugarcane bagasse, and potato peel) was the most appropriate substrate triggering co-production of orange and red monascus pigments; deduced from OVAT.
30532218	3	44	theme	substrate	935:943	arg1	co-production					956:968	the most appropriate substrate triggering co-production	914:968	the most appropriate substrate triggering co-production of orange and red monascus pigments	914:1004	Corn cob among other carbon sources (e.g., rice grains, sugarcane bagasse, and potato peel) was the most appropriate substrate triggering co-production of orange and red monascus pigments; deduced from OVAT.
30532218	5	45	theme	units/mL	1312:1319	arg1	value					1273:1277	a maximal color value	1257:1277	a maximal color value of 133.77 and 108.02 color value units/mL of orange and red pigments, respectively at 30 oC after 10 days	1257:1383	The optimal levels of inoculum size (12 x 1011 spores/mL) and glycerol (2.17 M) did achieve a maximal color value of 133.77 and 108.02 color value units/mL of orange and red pigments, respectively at 30 oC after 10 days; concluded from CCD with an agitation speed of 150 rpm.
30532218	1	46	theme	effective	253:261	arg1	cost					247:250	a statistically optimized, low cost	216:250	cost	247:250	The present study underlines a statistically optimized, low cost, effective approach for efficient co-valorization of two non-efficiently utilized, highly accumulated, raw agro-industrial wastes: corn cob and glycerol for co-production of natural biopigments: monascus orange and red pigments by the aid of Monascus purpureus strain ATCC 16436.
30532218	1	46	theme	effective	253:261	arg1	approach					263:270	effective approach	253:270	effective approach for efficient co-valorization of two non-efficiently utilized, highly accumulated, raw agro-industrial wastes: corn cob and glycerol for co-production of natural biopigments: monascus orange and red pigments by the aid of Monascus purpureus strain ATCC 16436	253:529	The present study underlines a statistically optimized, low cost, effective approach for efficient co-valorization of two non-efficiently utilized, highly accumulated, raw agro-industrial wastes: corn cob and glycerol for co-production of natural biopigments: monascus orange and red pigments by the aid of Monascus purpureus strain ATCC 16436.
30532218	3	47	theme	carbon	839:844	arg1	sources					846:852	other carbon sources	833:852	other carbon sources (e.g., rice grains, sugarcane bagasse, and potato peel)	833:908	Corn cob among other carbon sources (e.g., rice grains, sugarcane bagasse, and potato peel) was the most appropriate substrate triggering co-production of orange and red monascus pigments; deduced from OVAT.
30532218	3	48	theme	orange	973:978	arg1	cob					823:825	Corn cob	818:825	Corn cob among other carbon sources (e.g., rice grains, sugarcane bagasse, and potato peel)	818:908	Corn cob among other carbon sources (e.g., rice grains, sugarcane bagasse, and potato peel) was the most appropriate substrate triggering co-production of orange and red monascus pigments; deduced from OVAT.
30532218	3	48	theme	orange	973:978	arg1	co-production					956:968	the most appropriate substrate triggering co-production	914:968	the most appropriate substrate triggering co-production of orange and red monascus pigments	914:1004	Corn cob among other carbon sources (e.g., rice grains, sugarcane bagasse, and potato peel) was the most appropriate substrate triggering co-production of orange and red monascus pigments; deduced from OVAT.
30532218	4	49	theme	significant	1070:1080	arg1	P<0.05					1096:1101	P<0.05	1096:1101	P<0.05	1096:1101	Glycerol and inoculum size proved to impose significant consequences (P<0.05) on the production of monascus pigments as inferred from PBD.
30532218	4	49	theme	significant	1070:1080	arg1	consequences					1082:1093	significant consequences	1070:1093	significant consequences (P<0.05)	1070:1102	Glycerol and inoculum size proved to impose significant consequences (P<0.05) on the production of monascus pigments as inferred from PBD.
30532218	2	50	theme	pigments	736:743	arg1	production					713:722	the production	709:722	the production of monascus pigments using co-solid state fermentation of the two raw agro-industrial wastes	709:815	A three step sequential, statistical modeling approach: one variable at a time (OVAT), Plackett-Burman design (PBD), and central composite design (CCD) was employed to optimize the production of monascus pigments using co-solid state fermentation of the two raw agro-industrial wastes.
30532218	5	51	theme	color	1300:1304	arg1	units/mL					1312:1319	133.77 and 108.02 color value units/mL	1282:1319	133.77 and 108.02 color value units/mL	1282:1319	The optimal levels of inoculum size (12 x 1011 spores/mL) and glycerol (2.17 M) did achieve a maximal color value of 133.77 and 108.02 color value units/mL of orange and red pigments, respectively at 30 oC after 10 days; concluded from CCD with an agitation speed of 150 rpm.
30532218	1	52	theme	present	191:197	arg1	study					199:203	The present study	187:203	The present study	187:203	The present study underlines a statistically optimized, low cost, effective approach for efficient co-valorization of two non-efficiently utilized, highly accumulated, raw agro-industrial wastes: corn cob and glycerol for co-production of natural biopigments: monascus orange and red pigments by the aid of Monascus purpureus strain ATCC 16436.
30532218	6	53	theme	efficient	1548:1556	arg1	exploitation					1558:1569	efficient exploitation	1548:1569	efficient exploitation of such biopigments in food, pharmaceutical and textile industries	1548:1636	Present data would underpin the large scale production of monascus pigments using the present approach for efficient exploitation of such biopigments in food, pharmaceutical and textile industries.
30532218	2	54	theme	modeling	569:576	arg1	approach					578:585	A three step sequential, statistical modeling approach	532:585	A three step sequential, statistical modeling approach: one variable at a time (OVAT), Plackett-Burman design (PBD), and central composite design (CCD) was employed to optimize the production of monascus pigments using co-solid state fermentation of the two raw agro-industrial wastes.	532:816	A three step sequential, statistical modeling approach: one variable at a time (OVAT), Plackett-Burman design (PBD), and central composite design (CCD) was employed to optimize the production of monascus pigments using co-solid state fermentation of the two raw agro-industrial wastes.
30532218	2	55	theme	central	653:659	arg1	CCD					679:681	CCD	679:681	CCD	679:681	A three step sequential, statistical modeling approach: one variable at a time (OVAT), Plackett-Burman design (PBD), and central composite design (CCD) was employed to optimize the production of monascus pigments using co-solid state fermentation of the two raw agro-industrial wastes.
30532218	2	55	theme	central	653:659	arg1	design					671:676	central composite design	653:676	central composite design (CCD)	653:682	A three step sequential, statistical modeling approach: one variable at a time (OVAT), Plackett-Burman design (PBD), and central composite design (CCD) was employed to optimize the production of monascus pigments using co-solid state fermentation of the two raw agro-industrial wastes.
30532218	5	56	theme	size	1196:1199	arg1	levels					1177:1182	The optimal levels	1165:1182	The optimal levels of inoculum size (12 x 1011 spores/mL) and glycerol (2.17 M)	1165:1243	The optimal levels of inoculum size (12 x 1011 spores/mL) and glycerol (2.17 M) did achieve a maximal color value of 133.77 and 108.02 color value units/mL of orange and red pigments, respectively at 30 oC after 10 days; concluded from CCD with an agitation speed of 150 rpm.
30532218	0	57	theme	low	168:170	arg1	approach					177:184	An eco-friendly environmental low cost approach	138:184	Monascus orange and red pigments production by Monascus purpureus ATCC16436 through co-solid state fermentation of corn cob and glycerol: An eco-friendly environmental low cost approach.	0:185	Monascus orange and red pigments production by Monascus purpureus ATCC16436 through co-solid state fermentation of corn cob and glycerol: An eco-friendly environmental low cost approach.
30532218	3	58	theme	Corn	818:821	arg1	co-production					956:968	the most appropriate substrate triggering co-production	914:968	the most appropriate substrate triggering co-production of orange and red monascus pigments	914:1004	Corn cob among other carbon sources (e.g., rice grains, sugarcane bagasse, and potato peel) was the most appropriate substrate triggering co-production of orange and red monascus pigments; deduced from OVAT.
30532218	3	58	theme	Corn	818:821	arg1	cob					823:825	Corn cob	818:825	Corn cob among other carbon sources (e.g., rice grains, sugarcane bagasse, and potato peel)	818:908	Corn cob among other carbon sources (e.g., rice grains, sugarcane bagasse, and potato peel) was the most appropriate substrate triggering co-production of orange and red monascus pigments; deduced from OVAT.
30532218	6	59	from	industries	1627:1636	arg1	exploitation					1558:1569	efficient exploitation	1548:1569	efficient exploitation of such biopigments in food, pharmaceutical and textile industries	1548:1636	Present data would underpin the large scale production of monascus pigments using the present approach for efficient exploitation of such biopigments in food, pharmaceutical and textile industries.
30532218	6	60	theme	pigments	1508:1515	arg1	production					1485:1494	the large scale production	1469:1494	the large scale production of monascus pigments using the present approach for efficient exploitation of such biopigments in food, pharmaceutical and textile industries	1469:1636	Present data would underpin the large scale production of monascus pigments using the present approach for efficient exploitation of such biopigments in food, pharmaceutical and textile industries.
30532218	2	61	theme	monascus	727:734	arg1	pigments					736:743	monascus pigments	727:743	monascus pigments using co-solid state fermentation of the two raw agro-industrial wastes	727:815	A three step sequential, statistical modeling approach: one variable at a time (OVAT), Plackett-Burman design (PBD), and central composite design (CCD) was employed to optimize the production of monascus pigments using co-solid state fermentation of the two raw agro-industrial wastes.
30532218	1	62	theme	biopigments	434:444	arg1	co-production					409:421	co-production	409:421	co-production of natural biopigments	409:444	The present study underlines a statistically optimized, low cost, effective approach for efficient co-valorization of two non-efficiently utilized, highly accumulated, raw agro-industrial wastes: corn cob and glycerol for co-production of natural biopigments: monascus orange and red pigments by the aid of Monascus purpureus strain ATCC 16436.
30532218	3	63	dep	grains	866:871	arg1	e.g.					855:858	e.g.	855:858	e.g.	855:858	Corn cob among other carbon sources (e.g., rice grains, sugarcane bagasse, and potato peel) was the most appropriate substrate triggering co-production of orange and red monascus pigments; deduced from OVAT.
30532218	6	64	theme	pharmaceutical	1600:1613	arg1	industries					1627:1636	food, pharmaceutical and textile industries	1594:1636	food, pharmaceutical and textile industries	1594:1636	Present data would underpin the large scale production of monascus pigments using the present approach for efficient exploitation of such biopigments in food, pharmaceutical and textile industries.
30532218	5	65	theme	optimal	1169:1175	arg1	levels					1177:1182	The optimal levels	1165:1182	The optimal levels of inoculum size (12 x 1011 spores/mL) and glycerol (2.17 M)	1165:1243	The optimal levels of inoculum size (12 x 1011 spores/mL) and glycerol (2.17 M) did achieve a maximal color value of 133.77 and 108.02 color value units/mL of orange and red pigments, respectively at 30 oC after 10 days; concluded from CCD with an agitation speed of 150 rpm.
30532218	0	66	theme	co-solid	84:91	arg1	fermentation					99:110	co-solid state fermentation	84:110	co-solid state fermentation of corn cob and glycerol	84:135	Monascus orange and red pigments production by Monascus purpureus ATCC16436 through co-solid state fermentation of corn cob and glycerol: An eco-friendly environmental low cost approach.
30532218	3	67	theme	pigments	997:1004	arg1	cob					823:825	Corn cob	818:825	Corn cob among other carbon sources (e.g., rice grains, sugarcane bagasse, and potato peel)	818:908	Corn cob among other carbon sources (e.g., rice grains, sugarcane bagasse, and potato peel) was the most appropriate substrate triggering co-production of orange and red monascus pigments; deduced from OVAT.
30532218	3	67	theme	pigments	997:1004	arg1	co-production					956:968	the most appropriate substrate triggering co-production	914:968	the most appropriate substrate triggering co-production of orange and red monascus pigments	914:1004	Corn cob among other carbon sources (e.g., rice grains, sugarcane bagasse, and potato peel) was the most appropriate substrate triggering co-production of orange and red monascus pigments; deduced from OVAT.
30532218	1	68	dep	the	483:485	arg1	aid					487:489	aid	487:489	aid	487:489	The present study underlines a statistically optimized, low cost, effective approach for efficient co-valorization of two non-efficiently utilized, highly accumulated, raw agro-industrial wastes: corn cob and glycerol for co-production of natural biopigments: monascus orange and red pigments by the aid of Monascus purpureus strain ATCC 16436.
30532218	6	69	theme	food	1594:1597	arg1	industries					1627:1636	food, pharmaceutical and textile industries	1594:1636	food, pharmaceutical and textile industries	1594:1636	Present data would underpin the large scale production of monascus pigments using the present approach for efficient exploitation of such biopigments in food, pharmaceutical and textile industries.
30532218	3	70	theme	red	984:986	arg1	pigments					997:1004	red monascus pigments	984:1004	red monascus pigments	984:1004	Corn cob among other carbon sources (e.g., rice grains, sugarcane bagasse, and potato peel) was the most appropriate substrate triggering co-production of orange and red monascus pigments; deduced from OVAT.
30532218	6	71	from	exploitation	1558:1569	arg1	industries					1627:1636	food, pharmaceutical and textile industries	1594:1636	food, pharmaceutical and textile industries	1594:1636	Present data would underpin the large scale production of monascus pigments using the present approach for efficient exploitation of such biopigments in food, pharmaceutical and textile industries.
30532218	1	72	dep	utilized	325:332	arg1	accumulated					342:352	accumulated	342:352	accumulated	342:352	The present study underlines a statistically optimized, low cost, effective approach for efficient co-valorization of two non-efficiently utilized, highly accumulated, raw agro-industrial wastes: corn cob and glycerol for co-production of natural biopigments: monascus orange and red pigments by the aid of Monascus purpureus strain ATCC 16436.
30532218	1	72	dep	utilized	325:332	arg1	agro-industrial					359:373	agro-industrial	359:373	agro-industrial	359:373	The present study underlines a statistically optimized, low cost, effective approach for efficient co-valorization of two non-efficiently utilized, highly accumulated, raw agro-industrial wastes: corn cob and glycerol for co-production of natural biopigments: monascus orange and red pigments by the aid of Monascus purpureus strain ATCC 16436.
30532218	5	73	theme	glycerol	1227:1234	arg1	levels					1177:1182	The optimal levels	1165:1182	The optimal levels of inoculum size (12 x 1011 spores/mL) and glycerol (2.17 M)	1165:1243	The optimal levels of inoculum size (12 x 1011 spores/mL) and glycerol (2.17 M) did achieve a maximal color value of 133.77 and 108.02 color value units/mL of orange and red pigments, respectively at 30 oC after 10 days; concluded from CCD with an agitation speed of 150 rpm.
30532218	0	74	theme	corn	115:118	arg1	cob					120:122	corn cob	115:122	corn cob	115:122	Monascus orange and red pigments production by Monascus purpureus ATCC16436 through co-solid state fermentation of corn cob and glycerol: An eco-friendly environmental low cost approach.
30532218	1	75	theme	wastes	375:380	arg1	co-valorization					286:300	efficient co-valorization	276:300	efficient co-valorization of two non-efficiently utilized, highly accumulated, raw agro-industrial wastes	276:380	The present study underlines a statistically optimized, low cost, effective approach for efficient co-valorization of two non-efficiently utilized, highly accumulated, raw agro-industrial wastes: corn cob and glycerol for co-production of natural biopigments: monascus orange and red pigments by the aid of Monascus purpureus strain ATCC 16436.
30532218	6	76	theme	large	1473:1477	arg1	production					1485:1494	the large scale production	1469:1494	the large scale production of monascus pigments using the present approach for efficient exploitation of such biopigments in food, pharmaceutical and textile industries	1469:1636	Present data would underpin the large scale production of monascus pigments using the present approach for efficient exploitation of such biopigments in food, pharmaceutical and textile industries.
30532218	5	77	theme	red	1335:1337	arg1	pigments					1339:1346	red pigments	1335:1346	red pigments	1335:1346	The optimal levels of inoculum size (12 x 1011 spores/mL) and glycerol (2.17 M) did achieve a maximal color value of 133.77 and 108.02 color value units/mL of orange and red pigments, respectively at 30 oC after 10 days; concluded from CCD with an agitation speed of 150 rpm.
30532218	4	78	theme	pigments	1134:1141	arg1	production					1111:1120	the production	1107:1120	the production of monascus pigments	1107:1141	Glycerol and inoculum size proved to impose significant consequences (P<0.05) on the production of monascus pigments as inferred from PBD.
30532218	0	79	theme	orange	9:14	arg1	production					33:42	Monascus orange and red pigments production	0:42	Monascus orange and red pigments production by Monascus purpureus ATCC16436 through co-solid state fermentation of corn cob and glycerol: An eco-friendly environmental low cost approach.	0:185	Monascus orange and red pigments production by Monascus purpureus ATCC16436 through co-solid state fermentation of corn cob and glycerol: An eco-friendly environmental low cost approach.
30532218	1	80	theme	corn	383:386	arg1	cob					388:390	corn cob	383:390	corn cob	383:390	The present study underlines a statistically optimized, low cost, effective approach for efficient co-valorization of two non-efficiently utilized, highly accumulated, raw agro-industrial wastes: corn cob and glycerol for co-production of natural biopigments: monascus orange and red pigments by the aid of Monascus purpureus strain ATCC 16436.
30532218	5	81	theme	1011	1207:1210	arg1	spores/mL					1212:1220	12 x 1011 spores/mL	1202:1220	12 x 1011 spores/mL	1202:1220	The optimal levels of inoculum size (12 x 1011 spores/mL) and glycerol (2.17 M) did achieve a maximal color value of 133.77 and 108.02 color value units/mL of orange and red pigments, respectively at 30 oC after 10 days; concluded from CCD with an agitation speed of 150 rpm.
30532218	5	81	theme	1011	1207:1210	arg1	size					1196:1199	inoculum size	1187:1199	inoculum size (12 x 1011 spores/mL)	1187:1221	The optimal levels of inoculum size (12 x 1011 spores/mL) and glycerol (2.17 M) did achieve a maximal color value of 133.77 and 108.02 color value units/mL of orange and red pigments, respectively at 30 oC after 10 days; concluded from CCD with an agitation speed of 150 rpm.
30532218	0	82	theme	red	20:22	arg1	pigments					24:31	red pigments	20:31	red pigments	20:31	Monascus orange and red pigments production by Monascus purpureus ATCC16436 through co-solid state fermentation of corn cob and glycerol: An eco-friendly environmental low cost approach.
30532218	1	83	theme	efficient	276:284	arg1	co-valorization					286:300	efficient co-valorization	276:300	efficient co-valorization of two non-efficiently utilized, highly accumulated, raw agro-industrial wastes	276:380	The present study underlines a statistically optimized, low cost, effective approach for efficient co-valorization of two non-efficiently utilized, highly accumulated, raw agro-industrial wastes: corn cob and glycerol for co-production of natural biopigments: monascus orange and red pigments by the aid of Monascus purpureus strain ATCC 16436.
30532218	3	84	theme	sugarcane	874:882	arg1	bagasse					884:890	sugarcane bagasse	874:890	sugarcane bagasse	874:890	Corn cob among other carbon sources (e.g., rice grains, sugarcane bagasse, and potato peel) was the most appropriate substrate triggering co-production of orange and red monascus pigments; deduced from OVAT.
30532218	5	85	from	value	1273:1277	arg1	oC					1368:1369	30 oC	1365:1369	30 oC	1365:1369	The optimal levels of inoculum size (12 x 1011 spores/mL) and glycerol (2.17 M) did achieve a maximal color value of 133.77 and 108.02 color value units/mL of orange and red pigments, respectively at 30 oC after 10 days; concluded from CCD with an agitation speed of 150 rpm.
30532218	2	86	from	time	606:609	arg1	variable					592:599	one variable	588:599	one variable at a time (OVAT), Plackett-Burman design (PBD), and central composite design (CCD)	588:682	A three step sequential, statistical modeling approach: one variable at a time (OVAT), Plackett-Burman design (PBD), and central composite design (CCD) was employed to optimize the production of monascus pigments using co-solid state fermentation of the two raw agro-industrial wastes.
30532218	5	87	theme	maximal	1259:1265	arg1	value					1273:1277	a maximal color value	1257:1277	a maximal color value of 133.77 and 108.02 color value units/mL of orange and red pigments, respectively at 30 oC after 10 days	1257:1383	The optimal levels of inoculum size (12 x 1011 spores/mL) and glycerol (2.17 M) did achieve a maximal color value of 133.77 and 108.02 color value units/mL of orange and red pigments, respectively at 30 oC after 10 days; concluded from CCD with an agitation speed of 150 rpm.
30532218	0	88	dep	production	33:42	arg1	approach					177:184	An eco-friendly environmental low cost approach	138:184	Monascus orange and red pigments production by Monascus purpureus ATCC16436 through co-solid state fermentation of corn cob and glycerol: An eco-friendly environmental low cost approach.	0:185	Monascus orange and red pigments production by Monascus purpureus ATCC16436 through co-solid state fermentation of corn cob and glycerol: An eco-friendly environmental low cost approach.
30532218	3	89	theme	potato	897:902	arg1	peel					904:907	potato peel	897:907	potato peel	897:907	Corn cob among other carbon sources (e.g., rice grains, sugarcane bagasse, and potato peel) was the most appropriate substrate triggering co-production of orange and red monascus pigments; deduced from OVAT.
30532218	6	90	theme	textile	1619:1625	arg1	industries					1627:1636	food, pharmaceutical and textile industries	1594:1636	food, pharmaceutical and textile industries	1594:1636	Present data would underpin the large scale production of monascus pigments using the present approach for efficient exploitation of such biopigments in food, pharmaceutical and textile industries.
30532218	1	91	theme	Monascus	494:501	arg1	ATCC					520:523	Monascus purpureus strain ATCC 16436	494:529	Monascus purpureus strain ATCC 16436	494:529	The present study underlines a statistically optimized, low cost, effective approach for efficient co-valorization of two non-efficiently utilized, highly accumulated, raw agro-industrial wastes: corn cob and glycerol for co-production of natural biopigments: monascus orange and red pigments by the aid of Monascus purpureus strain ATCC 16436.
30532218	2	92	theme	state	760:764	arg1	fermentation					766:777	co-solid state fermentation	751:777	co-solid state fermentation of the two raw agro-industrial wastes	751:815	A three step sequential, statistical modeling approach: one variable at a time (OVAT), Plackett-Burman design (PBD), and central composite design (CCD) was employed to optimize the production of monascus pigments using co-solid state fermentation of the two raw agro-industrial wastes.
30532218	6	93	theme	Present	1441:1447	arg1	data					1449:1452	Present data	1441:1452	Present data	1441:1452	Present data would underpin the large scale production of monascus pigments using the present approach for efficient exploitation of such biopigments in food, pharmaceutical and textile industries.
30532218	1	94	theme	strain	513:518	arg1	ATCC					520:523	Monascus purpureus strain ATCC 16436	494:529	Monascus purpureus strain ATCC 16436	494:529	The present study underlines a statistically optimized, low cost, effective approach for efficient co-valorization of two non-efficiently utilized, highly accumulated, raw agro-industrial wastes: corn cob and glycerol for co-production of natural biopigments: monascus orange and red pigments by the aid of Monascus purpureus strain ATCC 16436.
30532218	1	95	theme	optimized	232:240	arg1	cost					247:250	a statistically optimized, low cost	216:250	cost	247:250	The present study underlines a statistically optimized, low cost, effective approach for efficient co-valorization of two non-efficiently utilized, highly accumulated, raw agro-industrial wastes: corn cob and glycerol for co-production of natural biopigments: monascus orange and red pigments by the aid of Monascus purpureus strain ATCC 16436.
30532218	1	95	theme	optimized	232:240	arg1	approach					263:270	effective approach	253:270	effective approach for efficient co-valorization of two non-efficiently utilized, highly accumulated, raw agro-industrial wastes: corn cob and glycerol for co-production of natural biopigments: monascus orange and red pigments by the aid of Monascus purpureus strain ATCC 16436	253:529	The present study underlines a statistically optimized, low cost, effective approach for efficient co-valorization of two non-efficiently utilized, highly accumulated, raw agro-industrial wastes: corn cob and glycerol for co-production of natural biopigments: monascus orange and red pigments by the aid of Monascus purpureus strain ATCC 16436.
30532218	6	96	theme	biopigments	1579:1589	arg1	exploitation					1558:1569	efficient exploitation	1548:1569	efficient exploitation of such biopigments in food, pharmaceutical and textile industries	1548:1636	Present data would underpin the large scale production of monascus pigments using the present approach for efficient exploitation of such biopigments in food, pharmaceutical and textile industries.
30532218	2	97	theme	wastes	810:815	arg1	fermentation					766:777	co-solid state fermentation	751:777	co-solid state fermentation of the two raw agro-industrial wastes	751:815	A three step sequential, statistical modeling approach: one variable at a time (OVAT), Plackett-Burman design (PBD), and central composite design (CCD) was employed to optimize the production of monascus pigments using co-solid state fermentation of the two raw agro-industrial wastes.
30532218	1	98	theme	low	243:245	arg1	cost					247:250	a statistically optimized, low cost	216:250	cost	247:250	The present study underlines a statistically optimized, low cost, effective approach for efficient co-valorization of two non-efficiently utilized, highly accumulated, raw agro-industrial wastes: corn cob and glycerol for co-production of natural biopigments: monascus orange and red pigments by the aid of Monascus purpureus strain ATCC 16436.
30532218	1	98	theme	low	243:245	arg1	approach					263:270	effective approach	253:270	effective approach for efficient co-valorization of two non-efficiently utilized, highly accumulated, raw agro-industrial wastes: corn cob and glycerol for co-production of natural biopigments: monascus orange and red pigments by the aid of Monascus purpureus strain ATCC 16436	253:529	The present study underlines a statistically optimized, low cost, effective approach for efficient co-valorization of two non-efficiently utilized, highly accumulated, raw agro-industrial wastes: corn cob and glycerol for co-production of natural biopigments: monascus orange and red pigments by the aid of Monascus purpureus strain ATCC 16436.
29323180	0	0	theme	fatty	73:77	arg1	acids					79:83	short-chain fatty acids	61:83	short-chain fatty acids	61:83	Low amounts of dietary fibre increase in vitro production of short-chain fatty acids without changing human colonic microbiota structure.
29323180	6	1	theme	low	1026:1028	arg1	amounts					1030:1036	such relatively low amounts	1010:1036	such relatively low amounts	1010:1036	Thus, even at such relatively low amounts, prebiotics appear capable of activating the metabolism of colonic microbiota.
29323180	2	2	used	used	380:383	arg2	We					377:378	We	377:378	We	377:378	We used an in vitro human colonic microbiota model based on batch fermentation starting from a faecal inoculum.
29323180	0	3	theme	short-chain	61:71	arg1	acids					79:83	short-chain fatty acids	61:83	short-chain fatty acids	61:83	Low amounts of dietary fibre increase in vitro production of short-chain fatty acids without changing human colonic microbiota structure.
29323180	1	4	theme	6 g	322:324	arg1	intake					312:317	a daily intake	304:317	a daily intake of 6 g of prebiotics per person (0.2% of dietary intake)	304:374	This study investigated the effect of various prebiotics (indigestible dextrin, α-cyclodextrin, and dextran) on human colonic microbiota at a dosage corresponding to a daily intake of 6 g of prebiotics per person (0.2% of dietary intake).
29323180	2	5	theme	faecal	472:477	arg1	inoculum					479:486	a faecal inoculum	470:486	a faecal inoculum	470:486	We used an in vitro human colonic microbiota model based on batch fermentation starting from a faecal inoculum.
29323180	6	6	theme	colonic	1097:1103	arg1	microbiota					1105:1114	colonic microbiota	1097:1114	colonic microbiota	1097:1114	Thus, even at such relatively low amounts, prebiotics appear capable of activating the metabolism of colonic microbiota.
29323180	3	7	theme	Bacterial	489:497	arg1	rRNA					503:506	Bacterial 16S rRNA	489:506	Bacterial 16S rRNA gene sequence analysis	489:529	Bacterial 16S rRNA gene sequence analysis showed that addition of 0.2% prebiotics did not change the diversity and composition of colonic microbiota.
29323180	1	8	theme	human	250:254	arg1	microbiota					264:273	human colonic microbiota	250:273	human colonic microbiota	250:273	This study investigated the effect of various prebiotics (indigestible dextrin, α-cyclodextrin, and dextran) on human colonic microbiota at a dosage corresponding to a daily intake of 6 g of prebiotics per person (0.2% of dietary intake).
29323180	0	9	theme	acids	79:83	arg1	production					47:56	in vitro production	38:56	in vitro production of short-chain fatty acids	38:83	Low amounts of dietary fibre increase in vitro production of short-chain fatty acids without changing human colonic microbiota structure.
29323180	1	10	theme	prebiotics	329:338	arg1	6 g					322:324	6 g	322:324	6 g of prebiotics per person (0.2% of dietary intake)	322:374	This study investigated the effect of various prebiotics (indigestible dextrin, α-cyclodextrin, and dextran) on human colonic microbiota at a dosage corresponding to a daily intake of 6 g of prebiotics per person (0.2% of dietary intake).
29323180	3	11	theme	16S	499:501	arg1	rRNA					503:506	Bacterial 16S rRNA	489:506	Bacterial 16S rRNA gene sequence analysis	489:529	Bacterial 16S rRNA gene sequence analysis showed that addition of 0.2% prebiotics did not change the diversity and composition of colonic microbiota.
29323180	1	12	theme	colonic	256:262	arg1	microbiota					264:273	human colonic microbiota	250:273	human colonic microbiota	250:273	This study investigated the effect of various prebiotics (indigestible dextrin, α-cyclodextrin, and dextran) on human colonic microbiota at a dosage corresponding to a daily intake of 6 g of prebiotics per person (0.2% of dietary intake).
29323180	0	13	theme	human	102:106	arg1	structure					127:135	human colonic microbiota structure	102:135	human colonic microbiota structure	102:135	Low amounts of dietary fibre increase in vitro production of short-chain fatty acids without changing human colonic microbiota structure.
29323180	4	14	theme	6 g	808:810	arg1	administration					790:803	administration	790:803	administration of 6 g of prebiotics	790:824	This finding coincided with results from a clinical study showing that the microbiota composition of human faecal samples remained unchanged following administration of 6 g of prebiotics over seven days.
29323180	4	15	theme	clinical	682:689	arg1	study					691:695	a clinical study	680:695	a clinical study showing that the microbiota composition of human faecal samples remained unchanged following administration of 6 g of prebiotics over seven days	680:840	This finding coincided with results from a clinical study showing that the microbiota composition of human faecal samples remained unchanged following administration of 6 g of prebiotics over seven days.
29323180	6	16	theme	microbiota	1105:1114	arg1	metabolism					1083:1092	the metabolism	1079:1092	the metabolism of colonic microbiota	1079:1114	Thus, even at such relatively low amounts, prebiotics appear capable of activating the metabolism of colonic microbiota.
29323180	6	17	theme	such	1010:1013	arg1	amounts					1030:1036	such relatively low amounts	1010:1036	such relatively low amounts	1010:1036	Thus, even at such relatively low amounts, prebiotics appear capable of activating the metabolism of colonic microbiota.
29323180	2	18	theme	human	397:401	arg1	model					422:426	an in vitro human colonic microbiota model	385:426	an in vitro human colonic microbiota model based on batch fermentation starting from a faecal inoculum	385:486	We used an in vitro human colonic microbiota model based on batch fermentation starting from a faecal inoculum.
29323180	2	19	theme	in	388:389	arg1	model					422:426	an in vitro human colonic microbiota model	385:426	an in vitro human colonic microbiota model based on batch fermentation starting from a faecal inoculum	385:486	We used an in vitro human colonic microbiota model based on batch fermentation starting from a faecal inoculum.
29323180	5	20	from	generation	935:944	arg1	system					988:993	the in vitro system	975:993	the in vitro system	975:993	However, compared to absence of prebiotics, their addition reduced the pH and increased the generation of acetate and propionate in the in vitro system.
29323180	5	21	dep	in	979:980	arg1	vitro					982:986	vitro	982:986	vitro	982:986	However, compared to absence of prebiotics, their addition reduced the pH and increased the generation of acetate and propionate in the in vitro system.
29323180	4	22	theme	prebiotics	815:824	arg1	6 g					808:810	6 g	808:810	6 g of prebiotics	808:824	This finding coincided with results from a clinical study showing that the microbiota composition of human faecal samples remained unchanged following administration of 6 g of prebiotics over seven days.
29323180	4	23	from	study	691:695	arg1	results					667:673	results	667:673	results from a clinical study showing that the microbiota composition of human faecal samples remained unchanged following administration of 6 g of prebiotics over seven days	667:840	This finding coincided with results from a clinical study showing that the microbiota composition of human faecal samples remained unchanged following administration of 6 g of prebiotics over seven days.
29323180	3	24	theme	sequence	513:520	arg1	analysis					522:529	Bacterial 16S rRNA gene sequence analysis	489:529	Bacterial 16S rRNA gene sequence analysis	489:529	Bacterial 16S rRNA gene sequence analysis showed that addition of 0.2% prebiotics did not change the diversity and composition of colonic microbiota.
29323180	1	25	theme	various	176:182	arg1	α-cyclodextrin					218:231	α-cyclodextrin	218:231	α-cyclodextrin	218:231	This study investigated the effect of various prebiotics (indigestible dextrin, α-cyclodextrin, and dextran) on human colonic microbiota at a dosage corresponding to a daily intake of 6 g of prebiotics per person (0.2% of dietary intake).
29323180	1	25	theme	various	176:182	arg1	prebiotics					184:193	various prebiotics	176:193	various prebiotics (indigestible dextrin, α-cyclodextrin, and dextran)	176:245	This study investigated the effect of various prebiotics (indigestible dextrin, α-cyclodextrin, and dextran) on human colonic microbiota at a dosage corresponding to a daily intake of 6 g of prebiotics per person (0.2% of dietary intake).
29323180	1	25	theme	various	176:182	arg1	dextrin					209:215	indigestible dextrin	196:215	indigestible dextrin	196:215	This study investigated the effect of various prebiotics (indigestible dextrin, α-cyclodextrin, and dextran) on human colonic microbiota at a dosage corresponding to a daily intake of 6 g of prebiotics per person (0.2% of dietary intake).
29323180	1	25	theme	various	176:182	arg1	dextran					238:244	dextran	238:244	dextran	238:244	This study investigated the effect of various prebiotics (indigestible dextrin, α-cyclodextrin, and dextran) on human colonic microbiota at a dosage corresponding to a daily intake of 6 g of prebiotics per person (0.2% of dietary intake).
29323180	0	26	theme	Low	0:2	arg1	amounts					4:10	Low amounts	0:10	Low amounts of dietary fibre	0:27	Low amounts of dietary fibre increase in vitro production of short-chain fatty acids without changing human colonic microbiota structure.
29323180	0	26	theme	Low	0:2	arg1	fibre					23:27	fibre	23:27	fibre	23:27	Low amounts of dietary fibre increase in vitro production of short-chain fatty acids without changing human colonic microbiota structure.
29323180	2	27	dep	in	388:389	arg1	vitro					391:395	vitro	391:395	vitro	391:395	We used an in vitro human colonic microbiota model based on batch fermentation starting from a faecal inoculum.
29323180	1	28	theme	prebiotics	184:193	arg1	effect					166:171	the effect	162:171	the effect of various prebiotics (indigestible dextrin, α-cyclodextrin, and dextran) on human colonic microbiota	162:273	This study investigated the effect of various prebiotics (indigestible dextrin, α-cyclodextrin, and dextran) on human colonic microbiota at a dosage corresponding to a daily intake of 6 g of prebiotics per person (0.2% of dietary intake).
29323180	4	29	theme	samples	753:759	arg1	composition					725:735	the microbiota composition	710:735	the microbiota composition of human faecal samples	710:759	This finding coincided with results from a clinical study showing that the microbiota composition of human faecal samples remained unchanged following administration of 6 g of prebiotics over seven days.
29323180	0	30	theme	microbiota	116:125	arg1	structure					127:135	human colonic microbiota structure	102:135	human colonic microbiota structure	102:135	Low amounts of dietary fibre increase in vitro production of short-chain fatty acids without changing human colonic microbiota structure.
29323180	3	31	theme	rRNA	503:506	arg1	analysis					522:529	Bacterial 16S rRNA gene sequence analysis	489:529	Bacterial 16S rRNA gene sequence analysis	489:529	Bacterial 16S rRNA gene sequence analysis showed that addition of 0.2% prebiotics did not change the diversity and composition of colonic microbiota.
29323180	4	32	theme	faecal	746:751	arg1	samples					753:759	human faecal samples	740:759	human faecal samples	740:759	This finding coincided with results from a clinical study showing that the microbiota composition of human faecal samples remained unchanged following administration of 6 g of prebiotics over seven days.
29323180	0	33	theme	colonic	108:114	arg1	structure					127:135	human colonic microbiota structure	102:135	human colonic microbiota structure	102:135	Low amounts of dietary fibre increase in vitro production of short-chain fatty acids without changing human colonic microbiota structure.
29323180	3	34	theme	gene	508:511	arg1	analysis					522:529	Bacterial 16S rRNA gene sequence analysis	489:529	Bacterial 16S rRNA gene sequence analysis	489:529	Bacterial 16S rRNA gene sequence analysis showed that addition of 0.2% prebiotics did not change the diversity and composition of colonic microbiota.
29323180	1	35	theme	indigestible	196:207	arg1	prebiotics					184:193	various prebiotics	176:193	various prebiotics (indigestible dextrin, α-cyclodextrin, and dextran)	176:245	This study investigated the effect of various prebiotics (indigestible dextrin, α-cyclodextrin, and dextran) on human colonic microbiota at a dosage corresponding to a daily intake of 6 g of prebiotics per person (0.2% of dietary intake).
29323180	1	35	theme	indigestible	196:207	arg1	dextrin					209:215	indigestible dextrin	196:215	indigestible dextrin	196:215	This study investigated the effect of various prebiotics (indigestible dextrin, α-cyclodextrin, and dextran) on human colonic microbiota at a dosage corresponding to a daily intake of 6 g of prebiotics per person (0.2% of dietary intake).
29323180	5	36	theme	propionate	961:970	arg1	generation					935:944	the generation	931:944	the generation of acetate and propionate in the in vitro system	931:993	However, compared to absence of prebiotics, their addition reduced the pH and increased the generation of acetate and propionate in the in vitro system.
29323180	0	37	theme	fibre	23:27	arg1	amounts					4:10	Low amounts	0:10	Low amounts of dietary fibre	0:27	Low amounts of dietary fibre increase in vitro production of short-chain fatty acids without changing human colonic microbiota structure.
29323180	0	37	theme	fibre	23:27	arg1	fibre					23:27	fibre	23:27	fibre	23:27	Low amounts of dietary fibre increase in vitro production of short-chain fatty acids without changing human colonic microbiota structure.
29323180	1	38	dep	person	344:349	arg1	%					355:355	0.2%	352:355	0.2% of dietary intake	352:373	This study investigated the effect of various prebiotics (indigestible dextrin, α-cyclodextrin, and dextran) on human colonic microbiota at a dosage corresponding to a daily intake of 6 g of prebiotics per person (0.2% of dietary intake).
29323180	1	38	dep	person	344:349	arg1	intake					368:373	dietary intake	360:373	dietary intake	360:373	This study investigated the effect of various prebiotics (indigestible dextrin, α-cyclodextrin, and dextran) on human colonic microbiota at a dosage corresponding to a daily intake of 6 g of prebiotics per person (0.2% of dietary intake).
29323180	2	39	theme	microbiota	411:420	arg1	model					422:426	an in vitro human colonic microbiota model	385:426	an in vitro human colonic microbiota model based on batch fermentation starting from a faecal inoculum	385:486	We used an in vitro human colonic microbiota model based on batch fermentation starting from a faecal inoculum.
29323180	0	40	dep	in	38:39	arg1	vitro					41:45	vitro	41:45	vitro	41:45	Low amounts of dietary fibre increase in vitro production of short-chain fatty acids without changing human colonic microbiota structure.
29323180	2	41	theme	colonic	403:409	arg1	model					422:426	an in vitro human colonic microbiota model	385:426	an in vitro human colonic microbiota model based on batch fermentation starting from a faecal inoculum	385:486	We used an in vitro human colonic microbiota model based on batch fermentation starting from a faecal inoculum.
29323180	1	42	theme	dietary	360:366	arg1	intake					368:373	dietary intake	360:373	dietary intake	360:373	This study investigated the effect of various prebiotics (indigestible dextrin, α-cyclodextrin, and dextran) on human colonic microbiota at a dosage corresponding to a daily intake of 6 g of prebiotics per person (0.2% of dietary intake).
29323180	0	43	theme	in	38:39	arg1	production					47:56	in vitro production	38:56	in vitro production of short-chain fatty acids	38:83	Low amounts of dietary fibre increase in vitro production of short-chain fatty acids without changing human colonic microbiota structure.
29323180	1	44	theme	intake	368:373	arg1	%					355:355	0.2%	352:355	0.2% of dietary intake	352:373	This study investigated the effect of various prebiotics (indigestible dextrin, α-cyclodextrin, and dextran) on human colonic microbiota at a dosage corresponding to a daily intake of 6 g of prebiotics per person (0.2% of dietary intake).
29323180	1	44	theme	intake	368:373	arg1	intake					368:373	dietary intake	360:373	dietary intake	360:373	This study investigated the effect of various prebiotics (indigestible dextrin, α-cyclodextrin, and dextran) on human colonic microbiota at a dosage corresponding to a daily intake of 6 g of prebiotics per person (0.2% of dietary intake).
29323180	5	45	theme	in	979:980	arg1	system					988:993	the in vitro system	975:993	the in vitro system	975:993	However, compared to absence of prebiotics, their addition reduced the pH and increased the generation of acetate and propionate in the in vitro system.
29323180	5	46	theme	acetate	949:955	arg1	generation					935:944	the generation	931:944	the generation of acetate and propionate in the in vitro system	931:993	However, compared to absence of prebiotics, their addition reduced the pH and increased the generation of acetate and propionate in the in vitro system.
29323180	1	47	theme	daily	306:310	arg1	intake					312:317	a daily intake	304:317	a daily intake of 6 g of prebiotics per person (0.2% of dietary intake)	304:374	This study investigated the effect of various prebiotics (indigestible dextrin, α-cyclodextrin, and dextran) on human colonic microbiota at a dosage corresponding to a daily intake of 6 g of prebiotics per person (0.2% of dietary intake).
29323180	3	48	theme	microbiota	627:636	arg1	composition					604:614	composition	604:614	composition	604:614	Bacterial 16S rRNA gene sequence analysis showed that addition of 0.2% prebiotics did not change the diversity and composition of colonic microbiota.
29323180	3	48	theme	microbiota	627:636	arg1	diversity					590:598	diversity	590:598	diversity	590:598	Bacterial 16S rRNA gene sequence analysis showed that addition of 0.2% prebiotics did not change the diversity and composition of colonic microbiota.
29323180	3	49	theme	colonic	619:625	arg1	microbiota					627:636	colonic microbiota	619:636	colonic microbiota	619:636	Bacterial 16S rRNA gene sequence analysis showed that addition of 0.2% prebiotics did not change the diversity and composition of colonic microbiota.
29323180	3	50	theme	prebiotics	560:569	arg1	addition					543:550	addition	543:550	addition of 0.2% prebiotics	543:569	Bacterial 16S rRNA gene sequence analysis showed that addition of 0.2% prebiotics did not change the diversity and composition of colonic microbiota.
29323180	1	51	dep	prebiotics	184:193	arg1	α-cyclodextrin					218:231	α-cyclodextrin	218:231	α-cyclodextrin	218:231	This study investigated the effect of various prebiotics (indigestible dextrin, α-cyclodextrin, and dextran) on human colonic microbiota at a dosage corresponding to a daily intake of 6 g of prebiotics per person (0.2% of dietary intake).
29323180	1	51	dep	prebiotics	184:193	arg1	dextran					238:244	dextran	238:244	dextran	238:244	This study investigated the effect of various prebiotics (indigestible dextrin, α-cyclodextrin, and dextran) on human colonic microbiota at a dosage corresponding to a daily intake of 6 g of prebiotics per person (0.2% of dietary intake).
29323180	1	51	dep	prebiotics	184:193	arg1	dextrin					209:215	indigestible dextrin	196:215	indigestible dextrin	196:215	This study investigated the effect of various prebiotics (indigestible dextrin, α-cyclodextrin, and dextran) on human colonic microbiota at a dosage corresponding to a daily intake of 6 g of prebiotics per person (0.2% of dietary intake).
29323180	1	51	dep	prebiotics	184:193	arg1	prebiotics					184:193	various prebiotics	176:193	various prebiotics (indigestible dextrin, α-cyclodextrin, and dextran)	176:245	This study investigated the effect of various prebiotics (indigestible dextrin, α-cyclodextrin, and dextran) on human colonic microbiota at a dosage corresponding to a daily intake of 6 g of prebiotics per person (0.2% of dietary intake).
29323180	5	52	theme	prebiotics	875:884	arg1	absence					864:870	absence	864:870	absence of prebiotics	864:884	However, compared to absence of prebiotics, their addition reduced the pH and increased the generation of acetate and propionate in the in vitro system.
29323180	4	53	theme	microbiota	714:723	arg1	composition					725:735	the microbiota composition	710:735	the microbiota composition of human faecal samples	710:759	This finding coincided with results from a clinical study showing that the microbiota composition of human faecal samples remained unchanged following administration of 6 g of prebiotics over seven days.
29323180	4	54	theme	human	740:744	arg1	samples					753:759	human faecal samples	740:759	human faecal samples	740:759	This finding coincided with results from a clinical study showing that the microbiota composition of human faecal samples remained unchanged following administration of 6 g of prebiotics over seven days.
29323180	3	55	theme	0.2	555:557	arg1	%					558:558	%	558:558	%	558:558	Bacterial 16S rRNA gene sequence analysis showed that addition of 0.2% prebiotics did not change the diversity and composition of colonic microbiota.
29323180	2	56	theme	batch	437:441	arg1	fermentation					443:454	batch fermentation	437:454	batch fermentation starting from a faecal inoculum	437:486	We used an in vitro human colonic microbiota model based on batch fermentation starting from a faecal inoculum.
29323180	3	57	theme	%	558:558	arg1	prebiotics					560:569	0.2% prebiotics	555:569	0.2% prebiotics	555:569	Bacterial 16S rRNA gene sequence analysis showed that addition of 0.2% prebiotics did not change the diversity and composition of colonic microbiota.
29323180	1	58	from	effect	166:171	arg1	microbiota					264:273	human colonic microbiota	250:273	human colonic microbiota	250:273	This study investigated the effect of various prebiotics (indigestible dextrin, α-cyclodextrin, and dextran) on human colonic microbiota at a dosage corresponding to a daily intake of 6 g of prebiotics per person (0.2% of dietary intake).
29323180	3	59	dep	diversity	590:598	arg1	the					586:588	the	586:588	the	586:588	Bacterial 16S rRNA gene sequence analysis showed that addition of 0.2% prebiotics did not change the diversity and composition of colonic microbiota.
29960950	10	0	theme	great	1788:1792	arg1	potential					1794:1802	great potential	1788:1802	great potential as a drug delivery system for increasing the solubility and oral bioavailability of a poorly soluble compound	1788:1912	On the whole, PM/HA/CS benefits from the advantages of three dimensional macroporous HA/CS foam and polymer micelles, and exhibits great potential as a drug delivery system for increasing the solubility and oral bioavailability of a poorly soluble compound, like CC.
29960950	9	1	theme	Sprague-Dawley	1636:1649	arg1	rats					1651:1654	fasted Sprague-Dawley rats	1629:1654	fasted Sprague-Dawley rats	1629:1654	The PM/HA/CS-based CC formulation produced approximately 1.9-fold increased bioavailability when compared to the marketed tablets (Blopress®) administered to fasted Sprague-Dawley rats.
29960950	6	2	theme	excellent	1181:1189	arg1	stability					1200:1208	an excellent physical stability	1178:1208	an excellent physical stability	1178:1208	Differential scanning calorimetry (DSC) analysis confirmed that CC-PM/HA/CS was present in an amorphous form and has an excellent physical stability.
29960950	10	3	theme	drug	1809:1812	arg1	system					1823:1828	a drug delivery system	1807:1828	a drug delivery system	1807:1828	On the whole, PM/HA/CS benefits from the advantages of three dimensional macroporous HA/CS foam and polymer micelles, and exhibits great potential as a drug delivery system for increasing the solubility and oral bioavailability of a poorly soluble compound, like CC.
29960950	1	4	theme	hydroxyapatite/	198:212	arg1	PM/HA/CS					263:270	PM/HA/CS	263:270	PM/HA/CS	263:270	In the current study, a novel three-dimensional macroporous hydroxyapatite/ chitosan foam (HA/CS)-supported polymer micelle (PM/HA/CS) was developed, and its potential as an oral drug delivery system to enhance the solubility and oral bioavailability of poorly soluble compounds was systemically studied.
29960950	1	4	theme	hydroxyapatite/	198:212	arg1	micelle					254:260	a novel three-dimensional macroporous hydroxyapatite/ chitosan foam (HA/CS)-supported polymer micelle	160:260	a novel three-dimensional macroporous hydroxyapatite/ chitosan foam (HA/CS)-supported polymer micelle (PM/HA/CS)	160:271	In the current study, a novel three-dimensional macroporous hydroxyapatite/ chitosan foam (HA/CS)-supported polymer micelle (PM/HA/CS) was developed, and its potential as an oral drug delivery system to enhance the solubility and oral bioavailability of poorly soluble compounds was systemically studied.
29960950	3	5	theme	chemical	563:570	arg1	template					663:670	a macropore template	651:670	a macropore template	651:670	Firstly, HA/CS foam was synthesized using a wet chemical co-precipitation approach and poly-(methyl methacrylate) colloidal crystals as a macropore template.
29960950	3	5	theme	chemical	563:570	arg1	approach					589:596	a wet chemical co-precipitation approach	557:596	a wet chemical co-precipitation approach	557:596	Firstly, HA/CS foam was synthesized using a wet chemical co-precipitation approach and poly-(methyl methacrylate) colloidal crystals as a macropore template.
29960950	3	5	theme	chemical	563:570	arg1	crystals					639:646	poly-(methyl methacrylate) colloidal crystals	602:646	poly-(methyl methacrylate) colloidal crystals	602:646	Firstly, HA/CS foam was synthesized using a wet chemical co-precipitation approach and poly-(methyl methacrylate) colloidal crystals as a macropore template.
29960950	1	6	theme	foam	223:226	arg1	PM/HA/CS					263:270	PM/HA/CS	263:270	PM/HA/CS	263:270	In the current study, a novel three-dimensional macroporous hydroxyapatite/ chitosan foam (HA/CS)-supported polymer micelle (PM/HA/CS) was developed, and its potential as an oral drug delivery system to enhance the solubility and oral bioavailability of poorly soluble compounds was systemically studied.
29960950	1	6	theme	foam	223:226	arg1	micelle					254:260	a novel three-dimensional macroporous hydroxyapatite/ chitosan foam (HA/CS)-supported polymer micelle	160:260	a novel three-dimensional macroporous hydroxyapatite/ chitosan foam (HA/CS)-supported polymer micelle (PM/HA/CS)	160:271	In the current study, a novel three-dimensional macroporous hydroxyapatite/ chitosan foam (HA/CS)-supported polymer micelle (PM/HA/CS) was developed, and its potential as an oral drug delivery system to enhance the solubility and oral bioavailability of poorly soluble compounds was systemically studied.
29960950	9	7	theme	PM/HA/CS-based	1475:1488	arg1	formulation					1493:1503	The PM/HA/CS-based CC formulation	1471:1503	The PM/HA/CS-based CC formulation produced approximately 1.9-fold	1471:1535	The PM/HA/CS-based CC formulation produced approximately 1.9-fold increased bioavailability when compared to the marketed tablets (Blopress®) administered to fasted Sprague-Dawley rats.
29960950	8	8	from	non-cytotoxic	1456:1468	arg1	addition					1416:1423	addition	1416:1423	addition	1416:1423	In addition, PM/HA/CS is almost completely non-cytotoxic.
29960950	1	9	theme	HA/CS	229:233	arg1	PM/HA/CS					263:270	PM/HA/CS	263:270	PM/HA/CS	263:270	In the current study, a novel three-dimensional macroporous hydroxyapatite/ chitosan foam (HA/CS)-supported polymer micelle (PM/HA/CS) was developed, and its potential as an oral drug delivery system to enhance the solubility and oral bioavailability of poorly soluble compounds was systemically studied.
29960950	1	9	theme	HA/CS	229:233	arg1	micelle					254:260	a novel three-dimensional macroporous hydroxyapatite/ chitosan foam (HA/CS)-supported polymer micelle	160:260	a novel three-dimensional macroporous hydroxyapatite/ chitosan foam (HA/CS)-supported polymer micelle (PM/HA/CS)	160:271	In the current study, a novel three-dimensional macroporous hydroxyapatite/ chitosan foam (HA/CS)-supported polymer micelle (PM/HA/CS) was developed, and its potential as an oral drug delivery system to enhance the solubility and oral bioavailability of poorly soluble compounds was systemically studied.
29960950	1	10	theme	soluble	399:405	arg1	compounds					407:415	poorly soluble compounds	392:415	poorly soluble compounds	392:415	In the current study, a novel three-dimensional macroporous hydroxyapatite/ chitosan foam (HA/CS)-supported polymer micelle (PM/HA/CS) was developed, and its potential as an oral drug delivery system to enhance the solubility and oral bioavailability of poorly soluble compounds was systemically studied.
29960950	3	11	theme	poly-	602:606	arg1	template					663:670	a macropore template	651:670	a macropore template	651:670	Firstly, HA/CS foam was synthesized using a wet chemical co-precipitation approach and poly-(methyl methacrylate) colloidal crystals as a macropore template.
29960950	3	11	theme	poly-	602:606	arg1	crystals					639:646	poly-(methyl methacrylate) colloidal crystals	602:646	poly-(methyl methacrylate) colloidal crystals	602:646	Firstly, HA/CS foam was synthesized using a wet chemical co-precipitation approach and poly-(methyl methacrylate) colloidal crystals as a macropore template.
29960950	3	11	theme	poly-	602:606	arg1	approach					589:596	a wet chemical co-precipitation approach	557:596	a wet chemical co-precipitation approach	557:596	Firstly, HA/CS foam was synthesized using a wet chemical co-precipitation approach and poly-(methyl methacrylate) colloidal crystals as a macropore template.
29960950	1	12	theme	-supported	235:244	arg1	PM/HA/CS					263:270	PM/HA/CS	263:270	PM/HA/CS	263:270	In the current study, a novel three-dimensional macroporous hydroxyapatite/ chitosan foam (HA/CS)-supported polymer micelle (PM/HA/CS) was developed, and its potential as an oral drug delivery system to enhance the solubility and oral bioavailability of poorly soluble compounds was systemically studied.
29960950	1	12	theme	-supported	235:244	arg1	micelle					254:260	a novel three-dimensional macroporous hydroxyapatite/ chitosan foam (HA/CS)-supported polymer micelle	160:260	a novel three-dimensional macroporous hydroxyapatite/ chitosan foam (HA/CS)-supported polymer micelle (PM/HA/CS)	160:271	In the current study, a novel three-dimensional macroporous hydroxyapatite/ chitosan foam (HA/CS)-supported polymer micelle (PM/HA/CS) was developed, and its potential as an oral drug delivery system to enhance the solubility and oral bioavailability of poorly soluble compounds was systemically studied.
29960950	5	13	theme	stability	1050:1058	arg1	terms					930:934	terms	930:934	terms of porous structure, morphology, angle of repose, crystallinity, drug loading, dissolution profiles, and physical stability	930:1058	The resulting CC-PM/HA/CS particles were then characterized in terms of porous structure, morphology, angle of repose, crystallinity, drug loading, dissolution profiles, and physical stability.
29960950	10	14	theme	oral	1864:1867	arg1	bioavailability					1869:1883	oral bioavailability	1864:1883	oral bioavailability	1864:1883	On the whole, PM/HA/CS benefits from the advantages of three dimensional macroporous HA/CS foam and polymer micelles, and exhibits great potential as a drug delivery system for increasing the solubility and oral bioavailability of a poorly soluble compound, like CC.
29960950	5	15	theme	porous	939:944	arg1	structure					946:954	porous structure	939:954	porous structure	939:954	The resulting CC-PM/HA/CS particles were then characterized in terms of porous structure, morphology, angle of repose, crystallinity, drug loading, dissolution profiles, and physical stability.
29960950	7	16	theme	pure	1395:1398	arg1	powder					1405:1410	the pure drug powder	1391:1410	the pure drug powder	1391:1410	Under both simulated gastric and intestinal conditions, the aqueous solubility and dissolution rate of the PM/HA/CS-based CC formulation were significantly increased compared with the pure drug powder.
29960950	10	17	theme	compound	1905:1912	arg1	solubility					1849:1858	solubility	1849:1858	solubility	1849:1858	On the whole, PM/HA/CS benefits from the advantages of three dimensional macroporous HA/CS foam and polymer micelles, and exhibits great potential as a drug delivery system for increasing the solubility and oral bioavailability of a poorly soluble compound, like CC.
29960950	10	17	theme	compound	1905:1912	arg1	bioavailability					1869:1883	oral bioavailability	1864:1883	oral bioavailability	1864:1883	On the whole, PM/HA/CS benefits from the advantages of three dimensional macroporous HA/CS foam and polymer micelles, and exhibits great potential as a drug delivery system for increasing the solubility and oral bioavailability of a poorly soluble compound, like CC.
29960950	5	18	theme	structure	946:954	arg1	terms					930:934	terms	930:934	terms of porous structure, morphology, angle of repose, crystallinity, drug loading, dissolution profiles, and physical stability	930:1058	The resulting CC-PM/HA/CS particles were then characterized in terms of porous structure, morphology, angle of repose, crystallinity, drug loading, dissolution profiles, and physical stability.
29960950	2	19	theme	model	503:507	arg1	cilexetil					455:463	Candesartan cilexetil	443:463	Candesartan cilexetil (CC)	443:468	Candesartan cilexetil (CC) was selected as a poorly soluble model drug.
29960950	2	19	theme	model	503:507	arg1	drug					509:512	a poorly soluble model drug	486:512	a poorly soluble model drug	486:512	Candesartan cilexetil (CC) was selected as a poorly soluble model drug.
29960950	6	20	located	present	1141:1147	arg2	CC-PM/HA/CS					1125:1135	CC-PM/HA/CS	1125:1135	CC-PM/HA/CS	1125:1135	Differential scanning calorimetry (DSC) analysis confirmed that CC-PM/HA/CS was present in an amorphous form and has an excellent physical stability.
29960950	6	20	located	present	1141:1147	arg1	form					1165:1168	an amorphous form	1152:1168	an amorphous form	1152:1168	Differential scanning calorimetry (DSC) analysis confirmed that CC-PM/HA/CS was present in an amorphous form and has an excellent physical stability.
29960950	6	21	theme	scanning	1074:1081	arg1	DSC					1096:1098	DSC	1096:1098	DSC	1096:1098	Differential scanning calorimetry (DSC) analysis confirmed that CC-PM/HA/CS was present in an amorphous form and has an excellent physical stability.
29960950	6	21	theme	scanning	1074:1081	arg1	calorimetry					1083:1093	Differential scanning calorimetry	1061:1093	Differential scanning calorimetry (DSC) analysis	1061:1108	Differential scanning calorimetry (DSC) analysis confirmed that CC-PM/HA/CS was present in an amorphous form and has an excellent physical stability.
29960950	0	22	theme	enhanced	90:97	arg1	delivery					104:111	enhanced oral delivery	90:111	enhanced oral delivery of poorly soluble drugs	90:135	Three dimensional macroporous hydroxyapatite/chitosan foam-supported polymer micelles for enhanced oral delivery of poorly soluble drugs.
29960950	3	23	theme	methyl	608:613	arg1	template					663:670	a macropore template	651:670	a macropore template	651:670	Firstly, HA/CS foam was synthesized using a wet chemical co-precipitation approach and poly-(methyl methacrylate) colloidal crystals as a macropore template.
29960950	3	23	theme	methyl	608:613	arg1	crystals					639:646	poly-(methyl methacrylate) colloidal crystals	602:646	poly-(methyl methacrylate) colloidal crystals	602:646	Firstly, HA/CS foam was synthesized using a wet chemical co-precipitation approach and poly-(methyl methacrylate) colloidal crystals as a macropore template.
29960950	3	23	theme	methyl	608:613	arg1	approach					589:596	a wet chemical co-precipitation approach	557:596	a wet chemical co-precipitation approach	557:596	Firstly, HA/CS foam was synthesized using a wet chemical co-precipitation approach and poly-(methyl methacrylate) colloidal crystals as a macropore template.
29960950	4	24	theme	polymer	701:707	arg1	micelles					709:716	the CC-loaded polymer micelles	687:716	the CC-loaded polymer micelles	687:716	Subsequently, the CC-loaded polymer micelles were efficiently encapsulated into the macropores of the HA/CS foam and freeze-dried to produce powdery CC-loaded PM/HA/CS composites (CC-PM/HA/CS).
29960950	1	25	theme	three-dimensional	168:184	arg1	PM/HA/CS					263:270	PM/HA/CS	263:270	PM/HA/CS	263:270	In the current study, a novel three-dimensional macroporous hydroxyapatite/ chitosan foam (HA/CS)-supported polymer micelle (PM/HA/CS) was developed, and its potential as an oral drug delivery system to enhance the solubility and oral bioavailability of poorly soluble compounds was systemically studied.
29960950	1	25	theme	three-dimensional	168:184	arg1	micelle					254:260	a novel three-dimensional macroporous hydroxyapatite/ chitosan foam (HA/CS)-supported polymer micelle	160:260	a novel three-dimensional macroporous hydroxyapatite/ chitosan foam (HA/CS)-supported polymer micelle (PM/HA/CS)	160:271	In the current study, a novel three-dimensional macroporous hydroxyapatite/ chitosan foam (HA/CS)-supported polymer micelle (PM/HA/CS) was developed, and its potential as an oral drug delivery system to enhance the solubility and oral bioavailability of poorly soluble compounds was systemically studied.
29960950	4	26	theme	HA/CS	775:779	arg1	foam					781:784	the HA/CS foam	771:784	the HA/CS foam	771:784	Subsequently, the CC-loaded polymer micelles were efficiently encapsulated into the macropores of the HA/CS foam and freeze-dried to produce powdery CC-loaded PM/HA/CS composites (CC-PM/HA/CS).
29960950	7	27	theme	simulated	1222:1230	arg1	conditions					1255:1264	both simulated gastric and intestinal conditions	1217:1264	both simulated gastric and intestinal conditions	1217:1264	Under both simulated gastric and intestinal conditions, the aqueous solubility and dissolution rate of the PM/HA/CS-based CC formulation were significantly increased compared with the pure drug powder.
29960950	7	28	theme	formulation	1336:1346	arg1	solubility					1279:1288	aqueous solubility	1271:1288	aqueous solubility	1271:1288	Under both simulated gastric and intestinal conditions, the aqueous solubility and dissolution rate of the PM/HA/CS-based CC formulation were significantly increased compared with the pure drug powder.
29960950	7	28	theme	formulation	1336:1346	arg1	rate					1306:1309	dissolution rate	1294:1309	dissolution rate	1294:1309	Under both simulated gastric and intestinal conditions, the aqueous solubility and dissolution rate of the PM/HA/CS-based CC formulation were significantly increased compared with the pure drug powder.
29960950	10	29	theme	macroporous	1730:1740	arg1	HA/CS					1742:1746	three dimensional macroporous HA/CS	1712:1746	three dimensional macroporous HA/CS foam and polymer micelles	1712:1772	On the whole, PM/HA/CS benefits from the advantages of three dimensional macroporous HA/CS foam and polymer micelles, and exhibits great potential as a drug delivery system for increasing the solubility and oral bioavailability of a poorly soluble compound, like CC.
29960950	0	30	theme	drugs	131:135	arg1	delivery					104:111	enhanced oral delivery	90:111	enhanced oral delivery of poorly soluble drugs	90:135	Three dimensional macroporous hydroxyapatite/chitosan foam-supported polymer micelles for enhanced oral delivery of poorly soluble drugs.
29960950	1	31	theme	oral	368:371	arg1	bioavailability					373:387	oral bioavailability	368:387	oral bioavailability	368:387	In the current study, a novel three-dimensional macroporous hydroxyapatite/ chitosan foam (HA/CS)-supported polymer micelle (PM/HA/CS) was developed, and its potential as an oral drug delivery system to enhance the solubility and oral bioavailability of poorly soluble compounds was systemically studied.
29960950	9	32	theme	fasted	1629:1634	arg1	rats					1651:1654	fasted Sprague-Dawley rats	1629:1654	fasted Sprague-Dawley rats	1629:1654	The PM/HA/CS-based CC formulation produced approximately 1.9-fold increased bioavailability when compared to the marketed tablets (Blopress®) administered to fasted Sprague-Dawley rats.
29960950	0	33	theme	oral	99:102	arg1	delivery					104:111	enhanced oral delivery	90:111	enhanced oral delivery of poorly soluble drugs	90:135	Three dimensional macroporous hydroxyapatite/chitosan foam-supported polymer micelles for enhanced oral delivery of poorly soluble drugs.
29960950	6	34	theme	calorimetry	1083:1093	arg1	analysis					1101:1108	Differential scanning calorimetry (DSC) analysis	1061:1108	Differential scanning calorimetry (DSC) analysis	1061:1108	Differential scanning calorimetry (DSC) analysis confirmed that CC-PM/HA/CS was present in an amorphous form and has an excellent physical stability.
29960950	10	35	theme	polymer	1757:1763	arg1	micelles					1765:1772	polymer micelles	1757:1772	polymer micelles	1757:1772	On the whole, PM/HA/CS benefits from the advantages of three dimensional macroporous HA/CS foam and polymer micelles, and exhibits great potential as a drug delivery system for increasing the solubility and oral bioavailability of a poorly soluble compound, like CC.
29960950	0	36	theme	macroporous	18:28	arg1	micelles					77:84	Three dimensional macroporous hydroxyapatite/chitosan foam-supported polymer micelles	0:84	Three dimensional macroporous hydroxyapatite/chitosan foam-supported polymer micelles for enhanced oral delivery of poorly soluble drugs.	0:136	Three dimensional macroporous hydroxyapatite/chitosan foam-supported polymer micelles for enhanced oral delivery of poorly soluble drugs.
29960950	10	37	theme	delivery	1814:1821	arg1	system					1823:1828	a drug delivery system	1807:1828	a drug delivery system	1807:1828	On the whole, PM/HA/CS benefits from the advantages of three dimensional macroporous HA/CS foam and polymer micelles, and exhibits great potential as a drug delivery system for increasing the solubility and oral bioavailability of a poorly soluble compound, like CC.
29960950	7	38	dep	solubility	1279:1288	arg1	the					1267:1269	the	1267:1269	the	1267:1269	Under both simulated gastric and intestinal conditions, the aqueous solubility and dissolution rate of the PM/HA/CS-based CC formulation were significantly increased compared with the pure drug powder.
29960950	6	39	theme	Differential	1061:1072	arg1	DSC					1096:1098	DSC	1096:1098	DSC	1096:1098	Differential scanning calorimetry (DSC) analysis confirmed that CC-PM/HA/CS was present in an amorphous form and has an excellent physical stability.
29960950	6	39	theme	Differential	1061:1072	arg1	calorimetry					1083:1093	Differential scanning calorimetry	1061:1093	Differential scanning calorimetry (DSC) analysis	1061:1108	Differential scanning calorimetry (DSC) analysis confirmed that CC-PM/HA/CS was present in an amorphous form and has an excellent physical stability.
29960950	4	40	theme	PM/HA/CS	832:839	arg1	CC-PM/HA/CS					853:863	CC-PM/HA/CS	853:863	CC-PM/HA/CS	853:863	Subsequently, the CC-loaded polymer micelles were efficiently encapsulated into the macropores of the HA/CS foam and freeze-dried to produce powdery CC-loaded PM/HA/CS composites (CC-PM/HA/CS).
29960950	4	40	theme	PM/HA/CS	832:839	arg1	composites					841:850	powdery CC-loaded PM/HA/CS composites	814:850	powdery CC-loaded PM/HA/CS composites (CC-PM/HA/CS)	814:864	Subsequently, the CC-loaded polymer micelles were efficiently encapsulated into the macropores of the HA/CS foam and freeze-dried to produce powdery CC-loaded PM/HA/CS composites (CC-PM/HA/CS).
29960950	1	41	theme	macroporous	186:196	arg1	PM/HA/CS					263:270	PM/HA/CS	263:270	PM/HA/CS	263:270	In the current study, a novel three-dimensional macroporous hydroxyapatite/ chitosan foam (HA/CS)-supported polymer micelle (PM/HA/CS) was developed, and its potential as an oral drug delivery system to enhance the solubility and oral bioavailability of poorly soluble compounds was systemically studied.
29960950	1	41	theme	macroporous	186:196	arg1	micelle					254:260	a novel three-dimensional macroporous hydroxyapatite/ chitosan foam (HA/CS)-supported polymer micelle	160:260	a novel three-dimensional macroporous hydroxyapatite/ chitosan foam (HA/CS)-supported polymer micelle (PM/HA/CS)	160:271	In the current study, a novel three-dimensional macroporous hydroxyapatite/ chitosan foam (HA/CS)-supported polymer micelle (PM/HA/CS) was developed, and its potential as an oral drug delivery system to enhance the solubility and oral bioavailability of poorly soluble compounds was systemically studied.
29960950	0	42	theme	foam-supported	54:67	arg1	micelles					77:84	Three dimensional macroporous hydroxyapatite/chitosan foam-supported polymer micelles	0:84	Three dimensional macroporous hydroxyapatite/chitosan foam-supported polymer micelles for enhanced oral delivery of poorly soluble drugs.	0:136	Three dimensional macroporous hydroxyapatite/chitosan foam-supported polymer micelles for enhanced oral delivery of poorly soluble drugs.
29960950	7	43	theme	PM/HA/CS-based	1318:1331	arg1	formulation					1336:1346	the PM/HA/CS-based CC formulation	1314:1346	the PM/HA/CS-based CC formulation	1314:1346	Under both simulated gastric and intestinal conditions, the aqueous solubility and dissolution rate of the PM/HA/CS-based CC formulation were significantly increased compared with the pure drug powder.
29960950	9	44	theme	CC	1490:1491	arg1	formulation					1493:1503	The PM/HA/CS-based CC formulation	1471:1503	The PM/HA/CS-based CC formulation produced approximately 1.9-fold	1471:1535	The PM/HA/CS-based CC formulation produced approximately 1.9-fold increased bioavailability when compared to the marketed tablets (Blopress®) administered to fasted Sprague-Dawley rats.
29960950	1	45	theme	drug	317:320	arg1	system					331:336	an oral drug delivery system	309:336	an oral drug delivery system	309:336	In the current study, a novel three-dimensional macroporous hydroxyapatite/ chitosan foam (HA/CS)-supported polymer micelle (PM/HA/CS) was developed, and its potential as an oral drug delivery system to enhance the solubility and oral bioavailability of poorly soluble compounds was systemically studied.
29960950	5	46	theme	physical	1041:1048	arg1	stability					1050:1058	physical stability	1041:1058	physical stability	1041:1058	The resulting CC-PM/HA/CS particles were then characterized in terms of porous structure, morphology, angle of repose, crystallinity, drug loading, dissolution profiles, and physical stability.
29960950	10	47	dep	HA/CS	1742:1746	arg1	foam					1748:1751	foam	1748:1751	foam	1748:1751	On the whole, PM/HA/CS benefits from the advantages of three dimensional macroporous HA/CS foam and polymer micelles, and exhibits great potential as a drug delivery system for increasing the solubility and oral bioavailability of a poorly soluble compound, like CC.
29960950	10	47	dep	HA/CS	1742:1746	arg1	micelles					1765:1772	polymer micelles	1757:1772	polymer micelles	1757:1772	On the whole, PM/HA/CS benefits from the advantages of three dimensional macroporous HA/CS foam and polymer micelles, and exhibits great potential as a drug delivery system for increasing the solubility and oral bioavailability of a poorly soluble compound, like CC.
29960950	1	48	theme	polymer	246:252	arg1	PM/HA/CS					263:270	PM/HA/CS	263:270	PM/HA/CS	263:270	In the current study, a novel three-dimensional macroporous hydroxyapatite/ chitosan foam (HA/CS)-supported polymer micelle (PM/HA/CS) was developed, and its potential as an oral drug delivery system to enhance the solubility and oral bioavailability of poorly soluble compounds was systemically studied.
29960950	1	48	theme	polymer	246:252	arg1	micelle					254:260	a novel three-dimensional macroporous hydroxyapatite/ chitosan foam (HA/CS)-supported polymer micelle	160:260	a novel three-dimensional macroporous hydroxyapatite/ chitosan foam (HA/CS)-supported polymer micelle (PM/HA/CS)	160:271	In the current study, a novel three-dimensional macroporous hydroxyapatite/ chitosan foam (HA/CS)-supported polymer micelle (PM/HA/CS) was developed, and its potential as an oral drug delivery system to enhance the solubility and oral bioavailability of poorly soluble compounds was systemically studied.
29960950	5	49	theme	loading	1006:1012	arg1	terms					930:934	terms	930:934	terms of porous structure, morphology, angle of repose, crystallinity, drug loading, dissolution profiles, and physical stability	930:1058	The resulting CC-PM/HA/CS particles were then characterized in terms of porous structure, morphology, angle of repose, crystallinity, drug loading, dissolution profiles, and physical stability.
29960950	7	50	theme	dissolution	1294:1304	arg1	rate					1306:1309	dissolution rate	1294:1309	dissolution rate	1294:1309	Under both simulated gastric and intestinal conditions, the aqueous solubility and dissolution rate of the PM/HA/CS-based CC formulation were significantly increased compared with the pure drug powder.
29960950	6	51	attach	present	1141:1147	arg2	CC-PM/HA/CS					1125:1135	CC-PM/HA/CS	1125:1135	CC-PM/HA/CS	1125:1135	Differential scanning calorimetry (DSC) analysis confirmed that CC-PM/HA/CS was present in an amorphous form and has an excellent physical stability.
29960950	6	51	attach	present	1141:1147	arg1	form					1165:1168	an amorphous form	1152:1168	an amorphous form	1152:1168	Differential scanning calorimetry (DSC) analysis confirmed that CC-PM/HA/CS was present in an amorphous form and has an excellent physical stability.
29960950	4	52	theme	powdery	814:820	arg1	CC-PM/HA/CS					853:863	CC-PM/HA/CS	853:863	CC-PM/HA/CS	853:863	Subsequently, the CC-loaded polymer micelles were efficiently encapsulated into the macropores of the HA/CS foam and freeze-dried to produce powdery CC-loaded PM/HA/CS composites (CC-PM/HA/CS).
29960950	4	52	theme	powdery	814:820	arg1	composites					841:850	powdery CC-loaded PM/HA/CS composites	814:850	powdery CC-loaded PM/HA/CS composites (CC-PM/HA/CS)	814:864	Subsequently, the CC-loaded polymer micelles were efficiently encapsulated into the macropores of the HA/CS foam and freeze-dried to produce powdery CC-loaded PM/HA/CS composites (CC-PM/HA/CS).
29960950	5	53	theme	CC-PM/HA/CS	881:891	arg1	particles					893:901	The resulting CC-PM/HA/CS particles	867:901	The resulting CC-PM/HA/CS particles	867:901	The resulting CC-PM/HA/CS particles were then characterized in terms of porous structure, morphology, angle of repose, crystallinity, drug loading, dissolution profiles, and physical stability.
29960950	5	54	theme	dissolution	1015:1025	arg1	profiles					1027:1034	dissolution profiles	1015:1034	dissolution profiles	1015:1034	The resulting CC-PM/HA/CS particles were then characterized in terms of porous structure, morphology, angle of repose, crystallinity, drug loading, dissolution profiles, and physical stability.
29960950	10	55	from	PM/HA/CS	1671:1678	arg1	whole					1664:1668	whole	1664:1668	whole	1664:1668	On the whole, PM/HA/CS benefits from the advantages of three dimensional macroporous HA/CS foam and polymer micelles, and exhibits great potential as a drug delivery system for increasing the solubility and oral bioavailability of a poorly soluble compound, like CC.
29960950	6	56	theme	physical	1191:1198	arg1	stability					1200:1208	an excellent physical stability	1178:1208	an excellent physical stability	1178:1208	Differential scanning calorimetry (DSC) analysis confirmed that CC-PM/HA/CS was present in an amorphous form and has an excellent physical stability.
29960950	6	57	contain	has	1174:1176	arg2	stability					1200:1208	an excellent physical stability	1178:1208	an excellent physical stability	1178:1208	Differential scanning calorimetry (DSC) analysis confirmed that CC-PM/HA/CS was present in an amorphous form and has an excellent physical stability.
29960950	6	57	contain	has	1174:1176	arg1	CC-PM/HA/CS					1125:1135	CC-PM/HA/CS	1125:1135	CC-PM/HA/CS	1125:1135	Differential scanning calorimetry (DSC) analysis confirmed that CC-PM/HA/CS was present in an amorphous form and has an excellent physical stability.
29960950	3	58	theme	HA/CS	524:528	arg1	foam					530:533	HA/CS foam	524:533	HA/CS foam	524:533	Firstly, HA/CS foam was synthesized using a wet chemical co-precipitation approach and poly-(methyl methacrylate) colloidal crystals as a macropore template.
29960950	5	59	theme	resulting	871:879	arg1	particles					893:901	The resulting CC-PM/HA/CS particles	867:901	The resulting CC-PM/HA/CS particles	867:901	The resulting CC-PM/HA/CS particles were then characterized in terms of porous structure, morphology, angle of repose, crystallinity, drug loading, dissolution profiles, and physical stability.
29960950	10	60	from	advantages	1698:1707	arg1	benefits					1680:1687	benefits	1680:1687	benefits from the advantages of three dimensional macroporous HA/CS foam and polymer micelles	1680:1772	On the whole, PM/HA/CS benefits from the advantages of three dimensional macroporous HA/CS foam and polymer micelles, and exhibits great potential as a drug delivery system for increasing the solubility and oral bioavailability of a poorly soluble compound, like CC.
29960950	3	61	theme	wet	559:561	arg1	template					663:670	a macropore template	651:670	a macropore template	651:670	Firstly, HA/CS foam was synthesized using a wet chemical co-precipitation approach and poly-(methyl methacrylate) colloidal crystals as a macropore template.
29960950	3	61	theme	wet	559:561	arg1	approach					589:596	a wet chemical co-precipitation approach	557:596	a wet chemical co-precipitation approach	557:596	Firstly, HA/CS foam was synthesized using a wet chemical co-precipitation approach and poly-(methyl methacrylate) colloidal crystals as a macropore template.
29960950	3	61	theme	wet	559:561	arg1	crystals					639:646	poly-(methyl methacrylate) colloidal crystals	602:646	poly-(methyl methacrylate) colloidal crystals	602:646	Firstly, HA/CS foam was synthesized using a wet chemical co-precipitation approach and poly-(methyl methacrylate) colloidal crystals as a macropore template.
29960950	1	62	theme	chitosan	214:221	arg1	PM/HA/CS					263:270	PM/HA/CS	263:270	PM/HA/CS	263:270	In the current study, a novel three-dimensional macroporous hydroxyapatite/ chitosan foam (HA/CS)-supported polymer micelle (PM/HA/CS) was developed, and its potential as an oral drug delivery system to enhance the solubility and oral bioavailability of poorly soluble compounds was systemically studied.
29960950	1	62	theme	chitosan	214:221	arg1	micelle					254:260	a novel three-dimensional macroporous hydroxyapatite/ chitosan foam (HA/CS)-supported polymer micelle	160:260	a novel three-dimensional macroporous hydroxyapatite/ chitosan foam (HA/CS)-supported polymer micelle (PM/HA/CS)	160:271	In the current study, a novel three-dimensional macroporous hydroxyapatite/ chitosan foam (HA/CS)-supported polymer micelle (PM/HA/CS) was developed, and its potential as an oral drug delivery system to enhance the solubility and oral bioavailability of poorly soluble compounds was systemically studied.
29960950	6	63	theme	amorphous	1155:1163	arg1	form					1165:1168	an amorphous form	1152:1168	an amorphous form	1152:1168	Differential scanning calorimetry (DSC) analysis confirmed that CC-PM/HA/CS was present in an amorphous form and has an excellent physical stability.
29960950	1	64	theme	compounds	407:415	arg1	solubility					353:362	solubility	353:362	solubility	353:362	In the current study, a novel three-dimensional macroporous hydroxyapatite/ chitosan foam (HA/CS)-supported polymer micelle (PM/HA/CS) was developed, and its potential as an oral drug delivery system to enhance the solubility and oral bioavailability of poorly soluble compounds was systemically studied.
29960950	1	64	theme	compounds	407:415	arg1	bioavailability					373:387	oral bioavailability	368:387	oral bioavailability	368:387	In the current study, a novel three-dimensional macroporous hydroxyapatite/ chitosan foam (HA/CS)-supported polymer micelle (PM/HA/CS) was developed, and its potential as an oral drug delivery system to enhance the solubility and oral bioavailability of poorly soluble compounds was systemically studied.
29960950	3	65	theme	co-precipitation	572:587	arg1	template					663:670	a macropore template	651:670	a macropore template	651:670	Firstly, HA/CS foam was synthesized using a wet chemical co-precipitation approach and poly-(methyl methacrylate) colloidal crystals as a macropore template.
29960950	3	65	theme	co-precipitation	572:587	arg1	approach					589:596	a wet chemical co-precipitation approach	557:596	a wet chemical co-precipitation approach	557:596	Firstly, HA/CS foam was synthesized using a wet chemical co-precipitation approach and poly-(methyl methacrylate) colloidal crystals as a macropore template.
29960950	3	65	theme	co-precipitation	572:587	arg1	crystals					639:646	poly-(methyl methacrylate) colloidal crystals	602:646	poly-(methyl methacrylate) colloidal crystals	602:646	Firstly, HA/CS foam was synthesized using a wet chemical co-precipitation approach and poly-(methyl methacrylate) colloidal crystals as a macropore template.
29960950	3	66	theme	methacrylate	615:626	arg1	template					663:670	a macropore template	651:670	a macropore template	651:670	Firstly, HA/CS foam was synthesized using a wet chemical co-precipitation approach and poly-(methyl methacrylate) colloidal crystals as a macropore template.
29960950	3	66	theme	methacrylate	615:626	arg1	crystals					639:646	poly-(methyl methacrylate) colloidal crystals	602:646	poly-(methyl methacrylate) colloidal crystals	602:646	Firstly, HA/CS foam was synthesized using a wet chemical co-precipitation approach and poly-(methyl methacrylate) colloidal crystals as a macropore template.
29960950	3	66	theme	methacrylate	615:626	arg1	approach					589:596	a wet chemical co-precipitation approach	557:596	a wet chemical co-precipitation approach	557:596	Firstly, HA/CS foam was synthesized using a wet chemical co-precipitation approach and poly-(methyl methacrylate) colloidal crystals as a macropore template.
29960950	1	67	theme	current	145:151	arg1	study					153:157	the current study	141:157	the current study	141:157	In the current study, a novel three-dimensional macroporous hydroxyapatite/ chitosan foam (HA/CS)-supported polymer micelle (PM/HA/CS) was developed, and its potential as an oral drug delivery system to enhance the solubility and oral bioavailability of poorly soluble compounds was systemically studied.
29960950	7	68	theme	drug	1400:1403	arg1	powder					1405:1410	the pure drug powder	1391:1410	the pure drug powder	1391:1410	Under both simulated gastric and intestinal conditions, the aqueous solubility and dissolution rate of the PM/HA/CS-based CC formulation were significantly increased compared with the pure drug powder.
29960950	10	69	dep	solubility	1849:1858	arg1	the					1845:1847	the	1845:1847	the	1845:1847	On the whole, PM/HA/CS benefits from the advantages of three dimensional macroporous HA/CS foam and polymer micelles, and exhibits great potential as a drug delivery system for increasing the solubility and oral bioavailability of a poorly soluble compound, like CC.
29960950	7	70	theme	intestinal	1244:1253	arg1	conditions					1255:1264	both simulated gastric and intestinal conditions	1217:1264	both simulated gastric and intestinal conditions	1217:1264	Under both simulated gastric and intestinal conditions, the aqueous solubility and dissolution rate of the PM/HA/CS-based CC formulation were significantly increased compared with the pure drug powder.
29960950	10	71	theme	soluble	1897:1903	arg1	compound					1905:1912	a poorly soluble compound	1888:1912	a poorly soluble compound	1888:1912	On the whole, PM/HA/CS benefits from the advantages of three dimensional macroporous HA/CS foam and polymer micelles, and exhibits great potential as a drug delivery system for increasing the solubility and oral bioavailability of a poorly soluble compound, like CC.
29960950	9	72	theme	marketed	1584:1591	arg1	tablets					1593:1599	the marketed tablets	1580:1599	the marketed tablets (Blopress®) administered to fasted Sprague-Dawley rats	1580:1654	The PM/HA/CS-based CC formulation produced approximately 1.9-fold increased bioavailability when compared to the marketed tablets (Blopress®) administered to fasted Sprague-Dawley rats.
29960950	9	72	theme	marketed	1584:1591	arg1	Blopress®					1602:1610	Blopress®	1602:1610	Blopress®	1602:1610	The PM/HA/CS-based CC formulation produced approximately 1.9-fold increased bioavailability when compared to the marketed tablets (Blopress®) administered to fasted Sprague-Dawley rats.
29960950	2	73	theme	soluble	495:501	arg1	cilexetil					455:463	Candesartan cilexetil	443:463	Candesartan cilexetil (CC)	443:468	Candesartan cilexetil (CC) was selected as a poorly soluble model drug.
29960950	2	73	theme	soluble	495:501	arg1	drug					509:512	a poorly soluble model drug	486:512	a poorly soluble model drug	486:512	Candesartan cilexetil (CC) was selected as a poorly soluble model drug.
29960950	1	74	dep	potential	296:304	arg1	enhance					341:347	enhance	341:347	to enhance the solubility and oral bioavailability of poorly soluble compounds	338:415	In the current study, a novel three-dimensional macroporous hydroxyapatite/ chitosan foam (HA/CS)-supported polymer micelle (PM/HA/CS) was developed, and its potential as an oral drug delivery system to enhance the solubility and oral bioavailability of poorly soluble compounds was systemically studied.
29960950	7	75	theme	gastric	1232:1238	arg1	conditions					1255:1264	both simulated gastric and intestinal conditions	1217:1264	both simulated gastric and intestinal conditions	1217:1264	Under both simulated gastric and intestinal conditions, the aqueous solubility and dissolution rate of the PM/HA/CS-based CC formulation were significantly increased compared with the pure drug powder.
29960950	1	76	theme	novel	162:166	arg1	PM/HA/CS					263:270	PM/HA/CS	263:270	PM/HA/CS	263:270	In the current study, a novel three-dimensional macroporous hydroxyapatite/ chitosan foam (HA/CS)-supported polymer micelle (PM/HA/CS) was developed, and its potential as an oral drug delivery system to enhance the solubility and oral bioavailability of poorly soluble compounds was systemically studied.
29960950	1	76	theme	novel	162:166	arg1	micelle					254:260	a novel three-dimensional macroporous hydroxyapatite/ chitosan foam (HA/CS)-supported polymer micelle	160:260	a novel three-dimensional macroporous hydroxyapatite/ chitosan foam (HA/CS)-supported polymer micelle (PM/HA/CS)	160:271	In the current study, a novel three-dimensional macroporous hydroxyapatite/ chitosan foam (HA/CS)-supported polymer micelle (PM/HA/CS) was developed, and its potential as an oral drug delivery system to enhance the solubility and oral bioavailability of poorly soluble compounds was systemically studied.
29960950	4	77	theme	CC-loaded	691:699	arg1	micelles					709:716	the CC-loaded polymer micelles	687:716	the CC-loaded polymer micelles	687:716	Subsequently, the CC-loaded polymer micelles were efficiently encapsulated into the macropores of the HA/CS foam and freeze-dried to produce powdery CC-loaded PM/HA/CS composites (CC-PM/HA/CS).
29960950	0	78	theme	soluble	123:129	arg1	drugs					131:135	poorly soluble drugs	116:135	poorly soluble drugs	116:135	Three dimensional macroporous hydroxyapatite/chitosan foam-supported polymer micelles for enhanced oral delivery of poorly soluble drugs.
29960950	5	79	theme	repose	978:983	arg1	stability					1050:1058	physical stability	1041:1058	physical stability	1041:1058	The resulting CC-PM/HA/CS particles were then characterized in terms of porous structure, morphology, angle of repose, crystallinity, drug loading, dissolution profiles, and physical stability.
29960950	5	79	theme	repose	978:983	arg1	structure					946:954	porous structure	939:954	porous structure	939:954	The resulting CC-PM/HA/CS particles were then characterized in terms of porous structure, morphology, angle of repose, crystallinity, drug loading, dissolution profiles, and physical stability.
29960950	5	79	theme	repose	978:983	arg1	crystallinity					986:998	crystallinity	986:998	crystallinity	986:998	The resulting CC-PM/HA/CS particles were then characterized in terms of porous structure, morphology, angle of repose, crystallinity, drug loading, dissolution profiles, and physical stability.
29960950	5	79	theme	repose	978:983	arg1	profiles					1027:1034	dissolution profiles	1015:1034	dissolution profiles	1015:1034	The resulting CC-PM/HA/CS particles were then characterized in terms of porous structure, morphology, angle of repose, crystallinity, drug loading, dissolution profiles, and physical stability.
29960950	5	79	theme	repose	978:983	arg1	morphology					957:966	morphology	957:966	morphology	957:966	The resulting CC-PM/HA/CS particles were then characterized in terms of porous structure, morphology, angle of repose, crystallinity, drug loading, dissolution profiles, and physical stability.
29960950	5	79	theme	repose	978:983	arg1	angle					969:973	angle	969:973	angle of repose	969:983	The resulting CC-PM/HA/CS particles were then characterized in terms of porous structure, morphology, angle of repose, crystallinity, drug loading, dissolution profiles, and physical stability.
29960950	5	79	theme	repose	978:983	arg1	loading					1006:1012	drug loading	1001:1012	drug loading	1001:1012	The resulting CC-PM/HA/CS particles were then characterized in terms of porous structure, morphology, angle of repose, crystallinity, drug loading, dissolution profiles, and physical stability.
29960950	5	80	theme	crystallinity	986:998	arg1	terms					930:934	terms	930:934	terms of porous structure, morphology, angle of repose, crystallinity, drug loading, dissolution profiles, and physical stability	930:1058	The resulting CC-PM/HA/CS particles were then characterized in terms of porous structure, morphology, angle of repose, crystallinity, drug loading, dissolution profiles, and physical stability.
29960950	4	81	theme	foam	781:784	arg1	macropores					757:766	the macropores	753:766	the macropores of the HA/CS foam	753:784	Subsequently, the CC-loaded polymer micelles were efficiently encapsulated into the macropores of the HA/CS foam and freeze-dried to produce powdery CC-loaded PM/HA/CS composites (CC-PM/HA/CS).
29960950	10	82	theme	dimensional	1718:1728	arg1	HA/CS					1742:1746	three dimensional macroporous HA/CS	1712:1746	three dimensional macroporous HA/CS foam and polymer micelles	1712:1772	On the whole, PM/HA/CS benefits from the advantages of three dimensional macroporous HA/CS foam and polymer micelles, and exhibits great potential as a drug delivery system for increasing the solubility and oral bioavailability of a poorly soluble compound, like CC.
29960950	0	83	theme	dimensional	6:16	arg1	micelles					77:84	Three dimensional macroporous hydroxyapatite/chitosan foam-supported polymer micelles	0:84	Three dimensional macroporous hydroxyapatite/chitosan foam-supported polymer micelles for enhanced oral delivery of poorly soluble drugs.	0:136	Three dimensional macroporous hydroxyapatite/chitosan foam-supported polymer micelles for enhanced oral delivery of poorly soluble drugs.
29960950	5	84	theme	morphology	957:966	arg1	terms					930:934	terms	930:934	terms of porous structure, morphology, angle of repose, crystallinity, drug loading, dissolution profiles, and physical stability	930:1058	The resulting CC-PM/HA/CS particles were then characterized in terms of porous structure, morphology, angle of repose, crystallinity, drug loading, dissolution profiles, and physical stability.
29960950	6	85	from	form	1165:1168	arg1	present					1141:1147	present	1141:1147	present	1141:1147	Differential scanning calorimetry (DSC) analysis confirmed that CC-PM/HA/CS was present in an amorphous form and has an excellent physical stability.
29960950	0	86	theme	hydroxyapatite/chitosan	30:52	arg1	micelles					77:84	Three dimensional macroporous hydroxyapatite/chitosan foam-supported polymer micelles	0:84	Three dimensional macroporous hydroxyapatite/chitosan foam-supported polymer micelles for enhanced oral delivery of poorly soluble drugs.	0:136	Three dimensional macroporous hydroxyapatite/chitosan foam-supported polymer micelles for enhanced oral delivery of poorly soluble drugs.
29960950	5	87	theme	angle	969:973	arg1	terms					930:934	terms	930:934	terms of porous structure, morphology, angle of repose, crystallinity, drug loading, dissolution profiles, and physical stability	930:1058	The resulting CC-PM/HA/CS particles were then characterized in terms of porous structure, morphology, angle of repose, crystallinity, drug loading, dissolution profiles, and physical stability.
29960950	7	88	theme	CC	1333:1334	arg1	formulation					1336:1346	the PM/HA/CS-based CC formulation	1314:1346	the PM/HA/CS-based CC formulation	1314:1346	Under both simulated gastric and intestinal conditions, the aqueous solubility and dissolution rate of the PM/HA/CS-based CC formulation were significantly increased compared with the pure drug powder.
29960950	2	89	theme	Candesartan	443:453	arg1	CC					466:467	CC	466:467	CC	466:467	Candesartan cilexetil (CC) was selected as a poorly soluble model drug.
29960950	2	89	theme	Candesartan	443:453	arg1	cilexetil					455:463	Candesartan cilexetil	443:463	Candesartan cilexetil (CC)	443:468	Candesartan cilexetil (CC) was selected as a poorly soluble model drug.
29960950	2	89	theme	Candesartan	443:453	arg1	drug					509:512	a poorly soluble model drug	486:512	a poorly soluble model drug	486:512	Candesartan cilexetil (CC) was selected as a poorly soluble model drug.
29960950	0	90	theme	polymer	69:75	arg1	micelles					77:84	Three dimensional macroporous hydroxyapatite/chitosan foam-supported polymer micelles	0:84	Three dimensional macroporous hydroxyapatite/chitosan foam-supported polymer micelles for enhanced oral delivery of poorly soluble drugs.	0:136	Three dimensional macroporous hydroxyapatite/chitosan foam-supported polymer micelles for enhanced oral delivery of poorly soluble drugs.
29960950	5	91	theme	profiles	1027:1034	arg1	terms					930:934	terms	930:934	terms of porous structure, morphology, angle of repose, crystallinity, drug loading, dissolution profiles, and physical stability	930:1058	The resulting CC-PM/HA/CS particles were then characterized in terms of porous structure, morphology, angle of repose, crystallinity, drug loading, dissolution profiles, and physical stability.
29960950	3	92	theme	colloidal	629:637	arg1	template					663:670	a macropore template	651:670	a macropore template	651:670	Firstly, HA/CS foam was synthesized using a wet chemical co-precipitation approach and poly-(methyl methacrylate) colloidal crystals as a macropore template.
29960950	3	92	theme	colloidal	629:637	arg1	crystals					639:646	poly-(methyl methacrylate) colloidal crystals	602:646	poly-(methyl methacrylate) colloidal crystals	602:646	Firstly, HA/CS foam was synthesized using a wet chemical co-precipitation approach and poly-(methyl methacrylate) colloidal crystals as a macropore template.
29960950	3	92	theme	colloidal	629:637	arg1	approach					589:596	a wet chemical co-precipitation approach	557:596	a wet chemical co-precipitation approach	557:596	Firstly, HA/CS foam was synthesized using a wet chemical co-precipitation approach and poly-(methyl methacrylate) colloidal crystals as a macropore template.
29960950	4	93	theme	CC-loaded	822:830	arg1	CC-PM/HA/CS					853:863	CC-PM/HA/CS	853:863	CC-PM/HA/CS	853:863	Subsequently, the CC-loaded polymer micelles were efficiently encapsulated into the macropores of the HA/CS foam and freeze-dried to produce powdery CC-loaded PM/HA/CS composites (CC-PM/HA/CS).
29960950	4	93	theme	CC-loaded	822:830	arg1	composites					841:850	powdery CC-loaded PM/HA/CS composites	814:850	powdery CC-loaded PM/HA/CS composites (CC-PM/HA/CS)	814:864	Subsequently, the CC-loaded polymer micelles were efficiently encapsulated into the macropores of the HA/CS foam and freeze-dried to produce powdery CC-loaded PM/HA/CS composites (CC-PM/HA/CS).
29960950	6	94	from	present	1141:1147	arg1	form					1165:1168	an amorphous form	1152:1168	an amorphous form	1152:1168	Differential scanning calorimetry (DSC) analysis confirmed that CC-PM/HA/CS was present in an amorphous form and has an excellent physical stability.
29960950	10	95	theme	HA/CS	1742:1746	arg1	advantages					1698:1707	the advantages	1694:1707	the advantages of three dimensional macroporous HA/CS foam and polymer micelles	1694:1772	On the whole, PM/HA/CS benefits from the advantages of three dimensional macroporous HA/CS foam and polymer micelles, and exhibits great potential as a drug delivery system for increasing the solubility and oral bioavailability of a poorly soluble compound, like CC.
29960950	1	96	theme	oral	312:315	arg1	system					331:336	an oral drug delivery system	309:336	an oral drug delivery system	309:336	In the current study, a novel three-dimensional macroporous hydroxyapatite/ chitosan foam (HA/CS)-supported polymer micelle (PM/HA/CS) was developed, and its potential as an oral drug delivery system to enhance the solubility and oral bioavailability of poorly soluble compounds was systemically studied.
29960950	1	97	dep	solubility	353:362	arg1	the					349:351	the	349:351	the	349:351	In the current study, a novel three-dimensional macroporous hydroxyapatite/ chitosan foam (HA/CS)-supported polymer micelle (PM/HA/CS) was developed, and its potential as an oral drug delivery system to enhance the solubility and oral bioavailability of poorly soluble compounds was systemically studied.
29960950	1	98	theme	delivery	322:329	arg1	system					331:336	an oral drug delivery system	309:336	an oral drug delivery system	309:336	In the current study, a novel three-dimensional macroporous hydroxyapatite/ chitosan foam (HA/CS)-supported polymer micelle (PM/HA/CS) was developed, and its potential as an oral drug delivery system to enhance the solubility and oral bioavailability of poorly soluble compounds was systemically studied.
29960950	5	99	theme	drug	1001:1004	arg1	loading					1006:1012	drug loading	1001:1012	drug loading	1001:1012	The resulting CC-PM/HA/CS particles were then characterized in terms of porous structure, morphology, angle of repose, crystallinity, drug loading, dissolution profiles, and physical stability.
29960950	3	100	theme	macropore	653:661	arg1	template					663:670	a macropore template	651:670	a macropore template	651:670	Firstly, HA/CS foam was synthesized using a wet chemical co-precipitation approach and poly-(methyl methacrylate) colloidal crystals as a macropore template.
29960950	3	100	theme	macropore	653:661	arg1	crystals					639:646	poly-(methyl methacrylate) colloidal crystals	602:646	poly-(methyl methacrylate) colloidal crystals	602:646	Firstly, HA/CS foam was synthesized using a wet chemical co-precipitation approach and poly-(methyl methacrylate) colloidal crystals as a macropore template.
29960950	3	100	theme	macropore	653:661	arg1	approach					589:596	a wet chemical co-precipitation approach	557:596	a wet chemical co-precipitation approach	557:596	Firstly, HA/CS foam was synthesized using a wet chemical co-precipitation approach and poly-(methyl methacrylate) colloidal crystals as a macropore template.
29960950	7	101	theme	aqueous	1271:1277	arg1	solubility					1279:1288	aqueous solubility	1271:1288	aqueous solubility	1271:1288	Under both simulated gastric and intestinal conditions, the aqueous solubility and dissolution rate of the PM/HA/CS-based CC formulation were significantly increased compared with the pure drug powder.
29960950	8	102	from	addition	1416:1423	arg1	non-cytotoxic					1456:1468	non-cytotoxic	1456:1468	non-cytotoxic	1456:1468	In addition, PM/HA/CS is almost completely non-cytotoxic.
30459196	6	0	theme	Point	885:889	arg1	mutations					891:899	Point mutations	885:899	Point mutations	885:899	Point mutations were introduced in the active site of Cda1 to generate strains in which the enzyme activity of Cda1 was abolished without perturbing either its stability or localization.
30459196	3	1	theme	virulence	490:498	arg1	determinant					500:510	an important virulence determinant	477:510	an important virulence determinant of C. neoformans Mutant strains deficient in chitosan are completely avirulent in a mouse pulmonary infection model	477:626	The functional role of chitosan in the fungal cell wall is not well defined, but it is an important virulence determinant of C. neoformans Mutant strains deficient in chitosan are completely avirulent in a mouse pulmonary infection model.
30459196	3	1	theme	virulence	490:498	arg1	it					471:472	it	471:472	it	471:472	The functional role of chitosan in the fungal cell wall is not well defined, but it is an important virulence determinant of C. neoformans Mutant strains deficient in chitosan are completely avirulent in a mouse pulmonary infection model.
30459196	9	2	theme	infection	1634:1642	arg1	reports					1623:1629	reports	1623:1629	reports of infection in hosts that are immunocompetent	1623:1676	Even though it causes serious infections in immunocompromised hosts, reports of infection in hosts that are immunocompetent are on the rise.
30459196	10	3	theme	fungal	1714:1719	arg1	pathogen					1721:1728	a fungal pathogen	1712:1728	a fungal pathogen	1712:1728	The cell wall of a fungal pathogen, its synthesis, composition, and pathways of remodelling are attractive therapeutic targets for the development of fungicides.
30459196	3	4	theme	neoformans	518:527	arg1	determinant					500:510	an important virulence determinant	477:510	an important virulence determinant of C. neoformans Mutant strains deficient in chitosan are completely avirulent in a mouse pulmonary infection model	477:626	The functional role of chitosan in the fungal cell wall is not well defined, but it is an important virulence determinant of C. neoformans Mutant strains deficient in chitosan are completely avirulent in a mouse pulmonary infection model.
30459196	3	4	theme	neoformans	518:527	arg1	it					471:472	it	471:472	it	471:472	The functional role of chitosan in the fungal cell wall is not well defined, but it is an important virulence determinant of C. neoformans Mutant strains deficient in chitosan are completely avirulent in a mouse pulmonary infection model.
30459196	1	5	theme	neoformans	169:178	arg1	wall					148:151	the cell wall	139:151	the cell wall of Cryptococcus neoformans conferring structural rigidity and integrity under diverse environmental conditions	139:262	Chitin is an essential component of the cell wall of Cryptococcus neoformans conferring structural rigidity and integrity under diverse environmental conditions.
30459196	8	6	theme	C.	1210:1211	arg1	genes					1228:1232	C. neoformans Cda genes	1210:1232	C. neoformans Cda genes	1210:1232	We further demonstrate that C. neoformans Cda genes are transcribed differently during a murine infection from what has been measured in vitroIMPORTANCECryptococcus neoformans is unique among fungal pathogens that cause disease in a mammalian host, as it secretes a polysaccharide capsule that hinders recognition by the host to facilitate its survival and proliferation.
30459196	10	7	theme	synthesis	1735:1743	arg1	targets					1814:1820	attractive therapeutic targets	1791:1820	attractive therapeutic targets for the development of fungicides	1791:1854	The cell wall of a fungal pathogen, its synthesis, composition, and pathways of remodelling are attractive therapeutic targets for the development of fungicides.
30459196	10	7	theme	synthesis	1735:1743	arg1	wall					1704:1707	The cell wall	1695:1707	The cell wall of a fungal pathogen, its synthesis, composition, and pathways of remodelling	1695:1785	The cell wall of a fungal pathogen, its synthesis, composition, and pathways of remodelling are attractive therapeutic targets for the development of fungicides.
30459196	1	8	theme	structural	191:200	arg1	rigidity					202:209	rigidity	202:209	rigidity	202:209	Chitin is an essential component of the cell wall of Cryptococcus neoformans conferring structural rigidity and integrity under diverse environmental conditions.
30459196	8	9	theme	Cda	1224:1226	arg1	genes					1228:1232	C. neoformans Cda genes	1210:1232	C. neoformans Cda genes	1210:1232	We further demonstrate that C. neoformans Cda genes are transcribed differently during a murine infection from what has been measured in vitroIMPORTANCECryptococcus neoformans is unique among fungal pathogens that cause disease in a mammalian host, as it secretes a polysaccharide capsule that hinders recognition by the host to facilitate its survival and proliferation.
30459196	2	10	theme	deacetylases	317:328	arg1	[Cdas					330:334	chitin deacetylases [Cdas]	310:335	chitin deacetylases [Cdas]	310:335	Chitin deacetylase genes encode the enyzmes (chitin deacetylases [Cdas]) that deacetylate chitin, converting it to chitosan.
30459196	2	10	theme	deacetylases	317:328	arg1	enyzmes					301:307	the enyzmes	297:307	the enyzmes (chitin deacetylases [Cdas]) that deacetylate chitin, converting it to chitosan	297:387	Chitin deacetylase genes encode the enyzmes (chitin deacetylases [Cdas]) that deacetylate chitin, converting it to chitosan.
30459196	6	11	theme	active	924:929	arg1	site					931:934	the active site	920:934	the active site of Cda1	920:942	Point mutations were introduced in the active site of Cda1 to generate strains in which the enzyme activity of Cda1 was abolished without perturbing either its stability or localization.
30459196	6	11	theme	active	924:929	arg1	Cda1					939:942	Cda1	939:942	Cda1	939:942	Point mutations were introduced in the active site of Cda1 to generate strains in which the enzyme activity of Cda1 was abolished without perturbing either its stability or localization.
30459196	8	12	theme	mammalian	1415:1423	arg1	host					1425:1428	a mammalian host	1413:1428	a mammalian host	1413:1428	We further demonstrate that C. neoformans Cda genes are transcribed differently during a murine infection from what has been measured in vitroIMPORTANCECryptococcus neoformans is unique among fungal pathogens that cause disease in a mammalian host, as it secretes a polysaccharide capsule that hinders recognition by the host to facilitate its survival and proliferation.
30459196	10	13	theme	composition	1746:1756	arg1	targets					1814:1820	attractive therapeutic targets	1791:1820	attractive therapeutic targets for the development of fungicides	1791:1854	The cell wall of a fungal pathogen, its synthesis, composition, and pathways of remodelling are attractive therapeutic targets for the development of fungicides.
30459196	10	13	theme	composition	1746:1756	arg1	wall					1704:1707	The cell wall	1695:1707	The cell wall of a fungal pathogen, its synthesis, composition, and pathways of remodelling	1695:1785	The cell wall of a fungal pathogen, its synthesis, composition, and pathways of remodelling are attractive therapeutic targets for the development of fungicides.
30459196	1	14	theme	essential	116:124	arg1	component					126:134	an essential component	113:134	an essential component of the cell wall of Cryptococcus neoformans conferring structural rigidity and integrity under diverse environmental conditions	113:262	Chitin is an essential component of the cell wall of Cryptococcus neoformans conferring structural rigidity and integrity under diverse environmental conditions.
30459196	1	14	theme	essential	116:124	arg1	Chitin					103:108	Chitin	103:108	Chitin	103:108	Chitin is an essential component of the cell wall of Cryptococcus neoformans conferring structural rigidity and integrity under diverse environmental conditions.
30459196	3	15	theme	infection	612:620	arg1	model					622:626	a mouse pulmonary infection model	594:626	a mouse pulmonary infection model	594:626	The functional role of chitosan in the fungal cell wall is not well defined, but it is an important virulence determinant of C. neoformans Mutant strains deficient in chitosan are completely avirulent in a mouse pulmonary infection model.
30459196	3	16	dep	determinant	500:510	arg1	avirulent					581:589	avirulent	581:589	avirulent	581:589	The functional role of chitosan in the fungal cell wall is not well defined, but it is an important virulence determinant of C. neoformans Mutant strains deficient in chitosan are completely avirulent in a mouse pulmonary infection model.
30459196	10	17	theme	pathogen	1721:1728	arg1	targets					1814:1820	attractive therapeutic targets	1791:1820	attractive therapeutic targets for the development of fungicides	1791:1854	The cell wall of a fungal pathogen, its synthesis, composition, and pathways of remodelling are attractive therapeutic targets for the development of fungicides.
30459196	10	17	theme	pathogen	1721:1728	arg1	wall					1704:1707	The cell wall	1695:1707	The cell wall of a fungal pathogen, its synthesis, composition, and pathways of remodelling	1695:1785	The cell wall of a fungal pathogen, its synthesis, composition, and pathways of remodelling are attractive therapeutic targets for the development of fungicides.
30459196	6	18	theme	enzyme	977:982	arg1	activity					984:991	the enzyme activity	973:991	the enzyme activity of Cda1	973:999	Point mutations were introduced in the active site of Cda1 to generate strains in which the enzyme activity of Cda1 was abolished without perturbing either its stability or localization.
30459196	3	19	theme	Mutant	529:534	arg1	strains					536:542	Mutant strains	529:542	Mutant strains deficient in chitosan	529:564	The functional role of chitosan in the fungal cell wall is not well defined, but it is an important virulence determinant of C. neoformans Mutant strains deficient in chitosan are completely avirulent in a mouse pulmonary infection model.
30459196	3	20	theme	pulmonary	602:610	arg1	model					622:626	a mouse pulmonary infection model	594:626	a mouse pulmonary infection model	594:626	The functional role of chitosan in the fungal cell wall is not well defined, but it is an important virulence determinant of C. neoformans Mutant strains deficient in chitosan are completely avirulent in a mouse pulmonary infection model.
30459196	5	21	theme	responsible	848:858	arg1	Cda					844:846	the principal Cda	830:846	the principal Cda responsible for fungal pathogenesis	830:882	Here we report that C. neoformans Cda1 is the principal Cda responsible for fungal pathogenesis.
30459196	5	21	theme	responsible	848:858	arg1	Cda1					822:825	C. neoformans Cda1	808:825	C. neoformans Cda1	808:825	Here we report that C. neoformans Cda1 is the principal Cda responsible for fungal pathogenesis.
30459196	11	22	from	polysaccharide	1869:1882	arg1	wall					1896:1899	the cell wall	1887:1899	the cell wall of C. neoformans	1887:1916	Chitosan, a polysaccharide in the cell wall of C. neoformans is one such target, as it is critical for pathogenesis and absent in the host.
30459196	5	23	theme	fungal	864:869	arg1	pathogenesis					871:882	fungal pathogenesis	864:882	fungal pathogenesis	864:882	Here we report that C. neoformans Cda1 is the principal Cda responsible for fungal pathogenesis.
30459196	12	24	theme	neoformans	2211:2220	arg1	pathogenesis					2192:2203	the pathogenesis	2188:2203	the pathogenesis of C. neoformans	2188:2220	The results we present shed light on the importance of one of the chitin deacetylases that synthesize chitosan during infection and further implicates chitosan as being a critical factor for the pathogenesis of C. neoformans.
30459196	11	25	from	host	1991:1994	arg1	absent					1977:1982	absent	1977:1982	absent	1977:1982	Chitosan, a polysaccharide in the cell wall of C. neoformans is one such target, as it is critical for pathogenesis and absent in the host.
30459196	12	26	theme	critical	2168:2175	arg1	factor					2177:2182	a critical factor	2166:2182	a critical factor for the pathogenesis of C. neoformans	2166:2220	The results we present shed light on the importance of one of the chitin deacetylases that synthesize chitosan during infection and further implicates chitosan as being a critical factor for the pathogenesis of C. neoformans.
30459196	4	27	dep	Cdas	675:678	arg1	Cda1					681:684	Cda1	681:684	Cda1	681:684	C. neoformans carries genes that encode three Cdas (Cda1, Cda2, and Cda3) that appear to be functionally redundant in cells grown under vegetative conditions.
30459196	4	27	dep	Cdas	675:678	arg1	Cdas					675:678	three Cdas	669:678	three Cdas (Cda1, Cda2, and Cda3) that appear to be functionally redundant in cells grown under vegetative conditions	669:785	C. neoformans carries genes that encode three Cdas (Cda1, Cda2, and Cda3) that appear to be functionally redundant in cells grown under vegetative conditions.
30459196	4	27	dep	Cdas	675:678	arg1	Cda2					687:690	Cda2	687:690	Cda2	687:690	C. neoformans carries genes that encode three Cdas (Cda1, Cda2, and Cda3) that appear to be functionally redundant in cells grown under vegetative conditions.
30459196	4	27	dep	Cdas	675:678	arg1	Cda3					697:700	Cda3	697:700	Cda3	697:700	C. neoformans carries genes that encode three Cdas (Cda1, Cda2, and Cda3) that appear to be functionally redundant in cells grown under vegetative conditions.
30459196	3	28	theme	functional	394:403	arg1	role					405:408	The functional role	390:408	The functional role of chitosan in the fungal cell wall	390:444	The functional role of chitosan in the fungal cell wall is not well defined, but it is an important virulence determinant of C. neoformans Mutant strains deficient in chitosan are completely avirulent in a mouse pulmonary infection model.
30459196	3	29	from	model	622:626	arg1	avirulent					581:589	avirulent	581:589	avirulent	581:589	The functional role of chitosan in the fungal cell wall is not well defined, but it is an important virulence determinant of C. neoformans Mutant strains deficient in chitosan are completely avirulent in a mouse pulmonary infection model.
30459196	3	30	theme	fungal	429:434	arg1	wall					441:444	the fungal cell wall	425:444	the fungal cell wall	425:444	The functional role of chitosan in the fungal cell wall is not well defined, but it is an important virulence determinant of C. neoformans Mutant strains deficient in chitosan are completely avirulent in a mouse pulmonary infection model.
30459196	0	31	theme	Cryptococcus	0:11	arg1	Cda1					24:27	Cda1	24:27	Cda1	24:27	Cryptococcus neoformans Cda1 and Its Chitin Deacetylase Activity Are Required for Fungal Pathogenesis.
30459196	0	31	theme	Cryptococcus	0:11	arg1	neoformans					13:22	Cryptococcus neoformans Cda1 and Its Chitin Deacetylase Activity	0:63	Cryptococcus neoformans Cda1 and Its Chitin Deacetylase Activity	0:63	Cryptococcus neoformans Cda1 and Its Chitin Deacetylase Activity Are Required for Fungal Pathogenesis.
30459196	0	31	theme	Cryptococcus	0:11	arg1	Activity					56:63	Its Chitin Deacetylase Activity	33:63	Its Chitin Deacetylase Activity	33:63	Cryptococcus neoformans Cda1 and Its Chitin Deacetylase Activity Are Required for Fungal Pathogenesis.
30459196	10	32	theme	remodelling	1775:1785	arg1	composition					1746:1756	composition	1746:1756	composition	1746:1756	The cell wall of a fungal pathogen, its synthesis, composition, and pathways of remodelling are attractive therapeutic targets for the development of fungicides.
30459196	10	32	theme	remodelling	1775:1785	arg1	pathogen					1721:1728	a fungal pathogen	1712:1728	a fungal pathogen	1712:1728	The cell wall of a fungal pathogen, its synthesis, composition, and pathways of remodelling are attractive therapeutic targets for the development of fungicides.
30459196	10	32	theme	remodelling	1775:1785	arg1	synthesis					1735:1743	its synthesis	1731:1743	its synthesis	1731:1743	The cell wall of a fungal pathogen, its synthesis, composition, and pathways of remodelling are attractive therapeutic targets for the development of fungicides.
30459196	10	32	theme	remodelling	1775:1785	arg1	pathways					1763:1770	pathways	1763:1770	pathways	1763:1770	The cell wall of a fungal pathogen, its synthesis, composition, and pathways of remodelling are attractive therapeutic targets for the development of fungicides.
30459196	3	33	from	avirulent	581:589	arg1	model					622:626	a mouse pulmonary infection model	594:626	a mouse pulmonary infection model	594:626	The functional role of chitosan in the fungal cell wall is not well defined, but it is an important virulence determinant of C. neoformans Mutant strains deficient in chitosan are completely avirulent in a mouse pulmonary infection model.
30459196	11	34	theme	cell	1891:1894	arg1	wall					1896:1899	the cell wall	1887:1899	the cell wall of C. neoformans	1887:1916	Chitosan, a polysaccharide in the cell wall of C. neoformans is one such target, as it is critical for pathogenesis and absent in the host.
30459196	4	35	contain	carries	643:649	arg1	neoformans					632:641	C. neoformans	629:641	C. neoformans	629:641	C. neoformans carries genes that encode three Cdas (Cda1, Cda2, and Cda3) that appear to be functionally redundant in cells grown under vegetative conditions.
30459196	4	35	contain	carries	643:649	arg2	Cdas					675:678	three Cdas	669:678	three Cdas (Cda1, Cda2, and Cda3) that appear to be functionally redundant in cells grown under vegetative conditions	669:785	C. neoformans carries genes that encode three Cdas (Cda1, Cda2, and Cda3) that appear to be functionally redundant in cells grown under vegetative conditions.
30459196	4	35	contain	carries	643:649	arg2	genes					651:655	genes	651:655	genes that encode three Cdas (Cda1, Cda2, and Cda3) that appear to be functionally redundant in cells grown under vegetative conditions	651:785	C. neoformans carries genes that encode three Cdas (Cda1, Cda2, and Cda3) that appear to be functionally redundant in cells grown under vegetative conditions.
30459196	8	36	theme	fungal	1374:1379	arg1	pathogens					1381:1389	fungal pathogens	1374:1389	fungal pathogens that cause disease in a mammalian host, as it secretes a polysaccharide capsule that hinders recognition by the host to facilitate its survival and proliferation	1374:1551	We further demonstrate that C. neoformans Cda genes are transcribed differently during a murine infection from what has been measured in vitroIMPORTANCECryptococcus neoformans is unique among fungal pathogens that cause disease in a mammalian host, as it secretes a polysaccharide capsule that hinders recognition by the host to facilitate its survival and proliferation.
30459196	7	37	theme	Cda1	1104:1107	arg1	strains					1116:1122	Cda1 mutant strains	1104:1122	Cda1 mutant strains	1104:1122	When used to infect CBA/J mice, Cda1 mutant strains produced less chitosan and were attenuated for virulence.
30459196	10	38	theme	therapeutic	1802:1812	arg1	targets					1814:1820	attractive therapeutic targets	1791:1820	attractive therapeutic targets for the development of fungicides	1791:1854	The cell wall of a fungal pathogen, its synthesis, composition, and pathways of remodelling are attractive therapeutic targets for the development of fungicides.
30459196	10	38	theme	therapeutic	1802:1812	arg1	wall					1704:1707	The cell wall	1695:1707	The cell wall of a fungal pathogen, its synthesis, composition, and pathways of remodelling	1695:1785	The cell wall of a fungal pathogen, its synthesis, composition, and pathways of remodelling are attractive therapeutic targets for the development of fungicides.
30459196	0	39	theme	Deacetylase	44:54	arg1	neoformans					13:22	Cryptococcus neoformans Cda1 and Its Chitin Deacetylase Activity	0:63	Cryptococcus neoformans Cda1 and Its Chitin Deacetylase Activity	0:63	Cryptococcus neoformans Cda1 and Its Chitin Deacetylase Activity Are Required for Fungal Pathogenesis.
30459196	0	39	theme	Deacetylase	44:54	arg1	Activity					56:63	Its Chitin Deacetylase Activity	33:63	Its Chitin Deacetylase Activity	33:63	Cryptococcus neoformans Cda1 and Its Chitin Deacetylase Activity Are Required for Fungal Pathogenesis.
30459196	2	40	theme	Chitin	265:270	arg1	genes					284:288	Chitin deacetylase genes	265:288	Chitin deacetylase genes	265:288	Chitin deacetylase genes encode the enyzmes (chitin deacetylases [Cdas]) that deacetylate chitin, converting it to chitosan.
30459196	2	40	theme	Chitin	265:270	arg1	enyzmes					301:307	the enyzmes	297:307	the enyzmes (chitin deacetylases [Cdas]) that deacetylate chitin, converting it to chitosan	297:387	Chitin deacetylase genes encode the enyzmes (chitin deacetylases [Cdas]) that deacetylate chitin, converting it to chitosan.
30459196	10	41	theme	fungicides	1845:1854	arg1	development					1830:1840	the development	1826:1840	the development of fungicides	1826:1854	The cell wall of a fungal pathogen, its synthesis, composition, and pathways of remodelling are attractive therapeutic targets for the development of fungicides.
30459196	5	42	dep	C.	808:809	arg1	neoformans					811:820	neoformans	811:820	neoformans	811:820	Here we report that C. neoformans Cda1 is the principal Cda responsible for fungal pathogenesis.
30459196	3	43	theme	mouse	596:600	arg1	model					622:626	a mouse pulmonary infection model	594:626	a mouse pulmonary infection model	594:626	The functional role of chitosan in the fungal cell wall is not well defined, but it is an important virulence determinant of C. neoformans Mutant strains deficient in chitosan are completely avirulent in a mouse pulmonary infection model.
30459196	1	44	theme	diverse	231:237	arg1	conditions					253:262	diverse environmental conditions	231:262	diverse environmental conditions	231:262	Chitin is an essential component of the cell wall of Cryptococcus neoformans conferring structural rigidity and integrity under diverse environmental conditions.
30459196	9	45	theme	immunocompromised	1598:1614	arg1	hosts					1616:1620	immunocompromised hosts	1598:1620	immunocompromised hosts	1598:1620	Even though it causes serious infections in immunocompromised hosts, reports of infection in hosts that are immunocompetent are on the rise.
30459196	1	46	theme	cell	143:146	arg1	wall					148:151	the cell wall	139:151	the cell wall of Cryptococcus neoformans conferring structural rigidity and integrity under diverse environmental conditions	139:262	Chitin is an essential component of the cell wall of Cryptococcus neoformans conferring structural rigidity and integrity under diverse environmental conditions.
30459196	4	47	theme	vegetative	765:774	arg1	conditions					776:785	vegetative conditions	765:785	vegetative conditions	765:785	C. neoformans carries genes that encode three Cdas (Cda1, Cda2, and Cda3) that appear to be functionally redundant in cells grown under vegetative conditions.
30459196	3	48	theme	important	480:488	arg1	determinant					500:510	an important virulence determinant	477:510	an important virulence determinant of C. neoformans Mutant strains deficient in chitosan are completely avirulent in a mouse pulmonary infection model	477:626	The functional role of chitosan in the fungal cell wall is not well defined, but it is an important virulence determinant of C. neoformans Mutant strains deficient in chitosan are completely avirulent in a mouse pulmonary infection model.
30459196	3	48	theme	important	480:488	arg1	it					471:472	it	471:472	it	471:472	The functional role of chitosan in the fungal cell wall is not well defined, but it is an important virulence determinant of C. neoformans Mutant strains deficient in chitosan are completely avirulent in a mouse pulmonary infection model.
30459196	2	49	theme	chitin	310:315	arg1	[Cdas					330:334	chitin deacetylases [Cdas]	310:335	chitin deacetylases [Cdas]	310:335	Chitin deacetylase genes encode the enyzmes (chitin deacetylases [Cdas]) that deacetylate chitin, converting it to chitosan.
30459196	2	49	theme	chitin	310:315	arg1	enyzmes					301:307	the enyzmes	297:307	the enyzmes (chitin deacetylases [Cdas]) that deacetylate chitin, converting it to chitosan	297:387	Chitin deacetylase genes encode the enyzmes (chitin deacetylases [Cdas]) that deacetylate chitin, converting it to chitosan.
30459196	1	50	theme	Cryptococcus	156:167	arg1	neoformans					169:178	Cryptococcus neoformans	156:178	Cryptococcus neoformans conferring structural rigidity and integrity under diverse environmental conditions	156:262	Chitin is an essential component of the cell wall of Cryptococcus neoformans conferring structural rigidity and integrity under diverse environmental conditions.
30459196	8	51	dep	neoformans	1347:1356	arg1	unique					1361:1366	unique	1361:1366	unique	1361:1366	We further demonstrate that C. neoformans Cda genes are transcribed differently during a murine infection from what has been measured in vitroIMPORTANCECryptococcus neoformans is unique among fungal pathogens that cause disease in a mammalian host, as it secretes a polysaccharide capsule that hinders recognition by the host to facilitate its survival and proliferation.
30459196	6	52	theme	Cda1	939:942	arg1	site					931:934	the active site	920:934	the active site of Cda1	920:942	Point mutations were introduced in the active site of Cda1 to generate strains in which the enzyme activity of Cda1 was abolished without perturbing either its stability or localization.
30459196	6	52	theme	Cda1	939:942	arg1	Cda1					939:942	Cda1	939:942	Cda1	939:942	Point mutations were introduced in the active site of Cda1 to generate strains in which the enzyme activity of Cda1 was abolished without perturbing either its stability or localization.
30459196	12	53	theme	one	2052:2054	arg1	importance					2038:2047	the importance	2034:2047	the importance of one of the chitin deacetylases that synthesize chitosan during infection and further implicates chitosan as being a critical factor for the pathogenesis of C. neoformans	2034:2220	The results we present shed light on the importance of one of the chitin deacetylases that synthesize chitosan during infection and further implicates chitosan as being a critical factor for the pathogenesis of C. neoformans.
30459196	3	54	theme	deficient	544:552	arg1	strains					536:542	Mutant strains	529:542	Mutant strains deficient in chitosan	529:564	The functional role of chitosan in the fungal cell wall is not well defined, but it is an important virulence determinant of C. neoformans Mutant strains deficient in chitosan are completely avirulent in a mouse pulmonary infection model.
30459196	11	55	theme	neoformans	1907:1916	arg1	wall					1896:1899	the cell wall	1887:1899	the cell wall of C. neoformans	1887:1916	Chitosan, a polysaccharide in the cell wall of C. neoformans is one such target, as it is critical for pathogenesis and absent in the host.
30459196	5	56	theme	principal	834:842	arg1	Cda					844:846	the principal Cda	830:846	the principal Cda responsible for fungal pathogenesis	830:882	Here we report that C. neoformans Cda1 is the principal Cda responsible for fungal pathogenesis.
30459196	5	56	theme	principal	834:842	arg1	Cda1					822:825	C. neoformans Cda1	808:825	C. neoformans Cda1	808:825	Here we report that C. neoformans Cda1 is the principal Cda responsible for fungal pathogenesis.
30459196	5	57	theme	C.	808:809	arg1	Cda					844:846	the principal Cda	830:846	the principal Cda responsible for fungal pathogenesis	830:882	Here we report that C. neoformans Cda1 is the principal Cda responsible for fungal pathogenesis.
30459196	5	57	theme	C.	808:809	arg1	Cda1					822:825	C. neoformans Cda1	808:825	C. neoformans Cda1	808:825	Here we report that C. neoformans Cda1 is the principal Cda responsible for fungal pathogenesis.
30459196	9	58	from	reports	1623:1629	arg1	hosts					1647:1651	hosts	1647:1651	hosts that are immunocompetent	1647:1676	Even though it causes serious infections in immunocompromised hosts, reports of infection in hosts that are immunocompetent are on the rise.
30459196	3	59	from	deficient	544:552	arg1	chitosan					557:564	chitosan	557:564	chitosan	557:564	The functional role of chitosan in the fungal cell wall is not well defined, but it is an important virulence determinant of C. neoformans Mutant strains deficient in chitosan are completely avirulent in a mouse pulmonary infection model.
30459196	12	60	theme	chitin	2063:2068	arg1	deacetylases					2070:2081	the chitin deacetylases	2059:2081	the chitin deacetylases that synthesize chitosan during infection and further implicates chitosan as being a critical factor for the pathogenesis of C. neoformans	2059:2220	The results we present shed light on the importance of one of the chitin deacetylases that synthesize chitosan during infection and further implicates chitosan as being a critical factor for the pathogenesis of C. neoformans.
30459196	10	61	theme	attractive	1791:1800	arg1	targets					1814:1820	attractive therapeutic targets	1791:1820	attractive therapeutic targets for the development of fungicides	1791:1854	The cell wall of a fungal pathogen, its synthesis, composition, and pathways of remodelling are attractive therapeutic targets for the development of fungicides.
30459196	10	61	theme	attractive	1791:1800	arg1	wall					1704:1707	The cell wall	1695:1707	The cell wall of a fungal pathogen, its synthesis, composition, and pathways of remodelling	1695:1785	The cell wall of a fungal pathogen, its synthesis, composition, and pathways of remodelling are attractive therapeutic targets for the development of fungicides.
30459196	8	62	theme	polysaccharide	1448:1461	arg1	capsule					1463:1469	a polysaccharide capsule	1446:1469	a polysaccharide capsule that hinders recognition by the host to facilitate its survival and proliferation	1446:1551	We further demonstrate that C. neoformans Cda genes are transcribed differently during a murine infection from what has been measured in vitroIMPORTANCECryptococcus neoformans is unique among fungal pathogens that cause disease in a mammalian host, as it secretes a polysaccharide capsule that hinders recognition by the host to facilitate its survival and proliferation.
30459196	3	63	from	chitosan	557:564	arg1	deficient					544:552	deficient	544:552	deficient	544:552	The functional role of chitosan in the fungal cell wall is not well defined, but it is an important virulence determinant of C. neoformans Mutant strains deficient in chitosan are completely avirulent in a mouse pulmonary infection model.
30459196	2	64	theme	deacetylase	272:282	arg1	genes					284:288	Chitin deacetylase genes	265:288	Chitin deacetylase genes	265:288	Chitin deacetylase genes encode the enyzmes (chitin deacetylases [Cdas]) that deacetylate chitin, converting it to chitosan.
30459196	2	64	theme	deacetylase	272:282	arg1	enyzmes					301:307	the enyzmes	297:307	the enyzmes (chitin deacetylases [Cdas]) that deacetylate chitin, converting it to chitosan	297:387	Chitin deacetylase genes encode the enyzmes (chitin deacetylases [Cdas]) that deacetylate chitin, converting it to chitosan.
30459196	8	65	theme	murine	1271:1276	arg1	infection					1278:1286	a murine infection	1269:1286	a murine infection from what has been measured in vitroIMPORTANCECryptococcus neoformans is unique among fungal pathogens that cause disease in a mammalian host, as it secretes a polysaccharide capsule that hinders recognition by the host to facilitate its survival and proliferation	1269:1551	We further demonstrate that C. neoformans Cda genes are transcribed differently during a murine infection from what has been measured in vitroIMPORTANCECryptococcus neoformans is unique among fungal pathogens that cause disease in a mammalian host, as it secretes a polysaccharide capsule that hinders recognition by the host to facilitate its survival and proliferation.
30459196	0	66	theme	Fungal	82:87	arg1	Pathogenesis					89:100	Fungal Pathogenesis	82:100	Fungal Pathogenesis	82:100	Cryptococcus neoformans Cda1 and Its Chitin Deacetylase Activity Are Required for Fungal Pathogenesis.
30459196	4	67	from	cells	747:751	arg1	redundant					734:742	redundant	734:742	redundant	734:742	C. neoformans carries genes that encode three Cdas (Cda1, Cda2, and Cda3) that appear to be functionally redundant in cells grown under vegetative conditions.
30459196	1	68	theme	wall	148:151	arg1	component					126:134	an essential component	113:134	an essential component of the cell wall of Cryptococcus neoformans conferring structural rigidity and integrity under diverse environmental conditions	113:262	Chitin is an essential component of the cell wall of Cryptococcus neoformans conferring structural rigidity and integrity under diverse environmental conditions.
30459196	1	68	theme	wall	148:151	arg1	Chitin					103:108	Chitin	103:108	Chitin	103:108	Chitin is an essential component of the cell wall of Cryptococcus neoformans conferring structural rigidity and integrity under diverse environmental conditions.
30459196	4	69	from	redundant	734:742	arg1	cells					747:751	cells	747:751	cells grown under vegetative conditions	747:785	C. neoformans carries genes that encode three Cdas (Cda1, Cda2, and Cda3) that appear to be functionally redundant in cells grown under vegetative conditions.
30459196	3	70	from	role	405:408	arg1	wall					441:444	the fungal cell wall	425:444	the fungal cell wall	425:444	The functional role of chitosan in the fungal cell wall is not well defined, but it is an important virulence determinant of C. neoformans Mutant strains deficient in chitosan are completely avirulent in a mouse pulmonary infection model.
30459196	8	71	dep	C.	1210:1211	arg1	neoformans					1213:1222	neoformans	1213:1222	neoformans	1213:1222	We further demonstrate that C. neoformans Cda genes are transcribed differently during a murine infection from what has been measured in vitroIMPORTANCECryptococcus neoformans is unique among fungal pathogens that cause disease in a mammalian host, as it secretes a polysaccharide capsule that hinders recognition by the host to facilitate its survival and proliferation.
30459196	3	72	theme	cell	436:439	arg1	wall					441:444	the fungal cell wall	425:444	the fungal cell wall	425:444	The functional role of chitosan in the fungal cell wall is not well defined, but it is an important virulence determinant of C. neoformans Mutant strains deficient in chitosan are completely avirulent in a mouse pulmonary infection model.
30459196	7	73	theme	mutant	1109:1114	arg1	strains					1116:1122	Cda1 mutant strains	1104:1122	Cda1 mutant strains	1104:1122	When used to infect CBA/J mice, Cda1 mutant strains produced less chitosan and were attenuated for virulence.
30459196	12	74	dep	results	2001:2007	arg1	present					2012:2018	present	2012:2018	present shed light on the importance of one of the chitin deacetylases that synthesize chitosan during infection and further implicates chitosan as being a critical factor for the pathogenesis of C. neoformans	2012:2220	The results we present shed light on the importance of one of the chitin deacetylases that synthesize chitosan during infection and further implicates chitosan as being a critical factor for the pathogenesis of C. neoformans.
30459196	10	75	theme	pathways	1763:1770	arg1	targets					1814:1820	attractive therapeutic targets	1791:1820	attractive therapeutic targets for the development of fungicides	1791:1854	The cell wall of a fungal pathogen, its synthesis, composition, and pathways of remodelling are attractive therapeutic targets for the development of fungicides.
30459196	10	75	theme	pathways	1763:1770	arg1	wall					1704:1707	The cell wall	1695:1707	The cell wall of a fungal pathogen, its synthesis, composition, and pathways of remodelling	1695:1785	The cell wall of a fungal pathogen, its synthesis, composition, and pathways of remodelling are attractive therapeutic targets for the development of fungicides.
30459196	0	76	theme	Chitin	37:42	arg1	neoformans					13:22	Cryptococcus neoformans Cda1 and Its Chitin Deacetylase Activity	0:63	Cryptococcus neoformans Cda1 and Its Chitin Deacetylase Activity	0:63	Cryptococcus neoformans Cda1 and Its Chitin Deacetylase Activity Are Required for Fungal Pathogenesis.
30459196	0	76	theme	Chitin	37:42	arg1	Activity					56:63	Its Chitin Deacetylase Activity	33:63	Its Chitin Deacetylase Activity	33:63	Cryptococcus neoformans Cda1 and Its Chitin Deacetylase Activity Are Required for Fungal Pathogenesis.
30459196	6	77	theme	Cda1	996:999	arg1	activity					984:991	the enzyme activity	973:991	the enzyme activity of Cda1	973:999	Point mutations were introduced in the active site of Cda1 to generate strains in which the enzyme activity of Cda1 was abolished without perturbing either its stability or localization.
30459196	7	78	theme	CBA/J	1092:1096	arg1	mice					1098:1101	CBA/J mice	1092:1101	CBA/J mice	1092:1101	When used to infect CBA/J mice, Cda1 mutant strains produced less chitosan and were attenuated for virulence.
30459196	9	79	theme	serious	1576:1582	arg1	infections					1584:1593	serious infections	1576:1593	serious infections	1576:1593	Even though it causes serious infections in immunocompromised hosts, reports of infection in hosts that are immunocompetent are on the rise.
30459196	3	80	theme	chitosan	413:420	arg1	role					405:408	The functional role	390:408	The functional role of chitosan in the fungal cell wall	390:444	The functional role of chitosan in the fungal cell wall is not well defined, but it is an important virulence determinant of C. neoformans Mutant strains deficient in chitosan are completely avirulent in a mouse pulmonary infection model.
30459196	1	81	theme	environmental	239:251	arg1	conditions					253:262	diverse environmental conditions	231:262	diverse environmental conditions	231:262	Chitin is an essential component of the cell wall of Cryptococcus neoformans conferring structural rigidity and integrity under diverse environmental conditions.
30459196	0	82	dep	neoformans	13:22	arg1	Cda1					24:27	Cda1	24:27	Cda1	24:27	Cryptococcus neoformans Cda1 and Its Chitin Deacetylase Activity Are Required for Fungal Pathogenesis.
30459196	0	82	dep	neoformans	13:22	arg1	neoformans					13:22	Cryptococcus neoformans Cda1 and Its Chitin Deacetylase Activity	0:63	Cryptococcus neoformans Cda1 and Its Chitin Deacetylase Activity	0:63	Cryptococcus neoformans Cda1 and Its Chitin Deacetylase Activity Are Required for Fungal Pathogenesis.
30459196	0	82	dep	neoformans	13:22	arg1	Activity					56:63	Its Chitin Deacetylase Activity	33:63	Its Chitin Deacetylase Activity	33:63	Cryptococcus neoformans Cda1 and Its Chitin Deacetylase Activity Are Required for Fungal Pathogenesis.
30459196	11	83	from	absent	1977:1982	arg1	host					1991:1994	the host	1987:1994	the host	1987:1994	Chitosan, a polysaccharide in the cell wall of C. neoformans is one such target, as it is critical for pathogenesis and absent in the host.
30459196	12	84	theme	deacetylases	2070:2081	arg1	deacetylases					2070:2081	the chitin deacetylases	2059:2081	the chitin deacetylases that synthesize chitosan during infection and further implicates chitosan as being a critical factor for the pathogenesis of C. neoformans	2059:2220	The results we present shed light on the importance of one of the chitin deacetylases that synthesize chitosan during infection and further implicates chitosan as being a critical factor for the pathogenesis of C. neoformans.
30459196	12	84	theme	deacetylases	2070:2081	arg1	one					2052:2054	one	2052:2054	one	2052:2054	The results we present shed light on the importance of one of the chitin deacetylases that synthesize chitosan during infection and further implicates chitosan as being a critical factor for the pathogenesis of C. neoformans.
30459196	10	85	theme	cell	1699:1702	arg1	targets					1814:1820	attractive therapeutic targets	1791:1820	attractive therapeutic targets for the development of fungicides	1791:1854	The cell wall of a fungal pathogen, its synthesis, composition, and pathways of remodelling are attractive therapeutic targets for the development of fungicides.
30459196	10	85	theme	cell	1699:1702	arg1	wall					1704:1707	The cell wall	1695:1707	The cell wall of a fungal pathogen, its synthesis, composition, and pathways of remodelling	1695:1785	The cell wall of a fungal pathogen, its synthesis, composition, and pathways of remodelling are attractive therapeutic targets for the development of fungicides.
31424381	4	0	dep	A.	703:704	arg1	enclensis					706:714	A. enclensis	703:714	A. enclensis	703:714	These data demonstrate that A. enclensis shares the characteristics of the genus Pseudarthrobacter.
31424381	2	1	theme	minor	406:410	arg1	H4					440:441	H4	440:441	H4	440:441	Arthrobacterenclensis was reported to cluster with species of the genus Pseudarthrobacter but the peptidoglycan containing lysine, alanine and glutamic acid and the presence of minor amounts of menaquinone MK-8(H4) were not in line with the description of the genus.
31424381	2	1	theme	minor	406:410	arg1	amounts					412:418	minor amounts	406:418	minor amounts of menaquinone MK-8(H4)	406:442	Arthrobacterenclensis was reported to cluster with species of the genus Pseudarthrobacter but the peptidoglycan containing lysine, alanine and glutamic acid and the presence of minor amounts of menaquinone MK-8(H4) were not in line with the description of the genus.
31424381	2	1	theme	minor	406:410	arg1	MK-8					435:438	menaquinone MK-8	423:438	menaquinone MK-8(H4)	423:442	Arthrobacterenclensis was reported to cluster with species of the genus Pseudarthrobacter but the peptidoglycan containing lysine, alanine and glutamic acid and the presence of minor amounts of menaquinone MK-8(H4) were not in line with the description of the genus.
31424381	10	2	dep	species	1836:1842	arg1	phenanthrenivorans					1847:1864	P. phenanthrenivorans	1844:1864	P. phenanthrenivorans	1844:1864	nov. and emend the descriptions of the genus Pseudarthrobacter and the two species P. phenanthrenivorans and P. scleromae.
31424381	10	2	dep	species	1836:1842	arg1	scleromae					1873:1881	P. scleromae	1870:1881	P. scleromae	1870:1881	nov. and emend the descriptions of the genus Pseudarthrobacter and the two species P. phenanthrenivorans and P. scleromae.
31424381	3	3	theme	25279T	667:672	arg1	system					640:645	the quinone system	628:645	the quinone system of A. enclensis DSM 25279T	628:672	Re-analysis of these traits revealed a peptidoglycan with l-Lys-l-Ser-l-Thr-l-Ala and no MK-8(H4), but major amounts of MK-9(H2) in the quinone system of A. enclensis DSM 25279T.
31424381	6	4	theme	polar	1251:1255	arg1	lipids					1257:1262	the polar lipids	1247:1262	the polar lipids of these two species	1247:1283	Since the polar lipid profile of P. phenanthrenivorans was reported to contain phosphatidylethanolamine, which is unusual for a member of the Micrococcaceae, and the polar lipid profile of P. scleromae was unknown, the polar lipids of these two species were also analysed.
31424381	8	5	theme	polar	1546:1550	arg1	profile					1558:1564	the polar lipid profile	1542:1564	the polar lipid profile of P. phenanthrenivorans DSM 18606T no phosphatidylethanolamine	1542:1628	In the polar lipid profile of P. phenanthrenivorans DSM 18606T no phosphatidylethanolamine could be detected.
31424381	9	6	theme	Pseudarthrobacterenclensis	1728:1753	arg1	reclassification					1692:1707	the reclassification	1688:1707	the reclassification of A. enclensis	1688:1723	Based on these results we here propose the reclassification of A. enclensis as Pseudarthrobacterenclensis comb.
31424381	9	6	theme	Pseudarthrobacterenclensis	1728:1753	arg1	comb					1755:1758	Pseudarthrobacterenclensis comb	1728:1758	Pseudarthrobacterenclensis comb	1728:1758	Based on these results we here propose the reclassification of A. enclensis as Pseudarthrobacterenclensis comb.
31424381	5	7	theme	quinone	979:985	arg1	systems					987:993	the quinone systems	975:993	the quinone systems of the two species	975:1012	Since the reported quinone systems of Pseudarthrobacterphenanthrenivorans [MK-8 and MK-9(H2)] and Pseudarthrobacterscleromae [MK-8(H2] were clearly different from those of other species of the genus, the quinone systems of the two species were re-analyzed.
31424381	3	8	with	peptidoglycan	535:547	arg1	H4					590:591	H4	590:591	H4	590:591	Re-analysis of these traits revealed a peptidoglycan with l-Lys-l-Ser-l-Thr-l-Ala and no MK-8(H4), but major amounts of MK-9(H2) in the quinone system of A. enclensis DSM 25279T.
31424381	3	8	with	peptidoglycan	535:547	arg1	l-Lys-l-Ser-l-Thr-l-Ala					554:576	l-Lys-l-Ser-l-Thr-l-Ala	554:576	l-Lys-l-Ser-l-Thr-l-Ala	554:576	Re-analysis of these traits revealed a peptidoglycan with l-Lys-l-Ser-l-Thr-l-Ala and no MK-8(H4), but major amounts of MK-9(H2) in the quinone system of A. enclensis DSM 25279T.
31424381	3	8	with	peptidoglycan	535:547	arg1	MK-8					585:588	no MK-8	582:588	no MK-8(H4)	582:592	Re-analysis of these traits revealed a peptidoglycan with l-Lys-l-Ser-l-Thr-l-Ala and no MK-8(H4), but major amounts of MK-9(H2) in the quinone system of A. enclensis DSM 25279T.
31424381	6	9	theme	lipid	1204:1208	arg1	unknown					1238:1244	unknown	1238:1244	unknown	1238:1244	Since the polar lipid profile of P. phenanthrenivorans was reported to contain phosphatidylethanolamine, which is unusual for a member of the Micrococcaceae, and the polar lipid profile of P. scleromae was unknown, the polar lipids of these two species were also analysed.
31424381	6	9	theme	lipid	1204:1208	arg1	profile					1210:1216	the polar lipid profile	1194:1216	the polar lipid profile of P. scleromae	1194:1232	Since the polar lipid profile of P. phenanthrenivorans was reported to contain phosphatidylethanolamine, which is unusual for a member of the Micrococcaceae, and the polar lipid profile of P. scleromae was unknown, the polar lipids of these two species were also analysed.
31424381	4	10	theme	genus	750:754	arg1	Pseudarthrobacter					756:772	the genus Pseudarthrobacter	746:772	the genus Pseudarthrobacter	746:772	These data demonstrate that A. enclensis shares the characteristics of the genus Pseudarthrobacter.
31424381	7	11	theme	scleromae	1447:1455	arg1	17756T					1461:1466	P. scleromae DSM 17756T	1444:1466	P. scleromae DSM 17756T	1444:1466	The quinone system of P. phenanthrenivorans DSM 18606T was composed of the major menaquinones MK-9(H2), MK-8(H2) and MK-10(H2) and that of P. scleromae DSM 17756T was composed of the major menaquinones MK-9(H2) and MK-8(H2) and MK-9.
31424381	2	12	theme	glutamic	372:379	arg1	acid					381:384	glutamic acid	372:384	glutamic acid	372:384	Arthrobacterenclensis was reported to cluster with species of the genus Pseudarthrobacter but the peptidoglycan containing lysine, alanine and glutamic acid and the presence of minor amounts of menaquinone MK-8(H4) were not in line with the description of the genus.
31424381	7	13	dep	menaquinones	1494:1505	arg1	H2					1525:1526	H2	1525:1526	H2	1525:1526	The quinone system of P. phenanthrenivorans DSM 18606T was composed of the major menaquinones MK-9(H2), MK-8(H2) and MK-10(H2) and that of P. scleromae DSM 17756T was composed of the major menaquinones MK-9(H2) and MK-8(H2) and MK-9.
31424381	7	13	dep	menaquinones	1494:1505	arg1	H2					1512:1513	H2	1512:1513	H2	1512:1513	The quinone system of P. phenanthrenivorans DSM 18606T was composed of the major menaquinones MK-9(H2), MK-8(H2) and MK-10(H2) and that of P. scleromae DSM 17756T was composed of the major menaquinones MK-9(H2) and MK-8(H2) and MK-9.
31424381	7	13	dep	menaquinones	1494:1505	arg1	MK-9					1533:1536	MK-9	1533:1536	MK-9	1533:1536	The quinone system of P. phenanthrenivorans DSM 18606T was composed of the major menaquinones MK-9(H2), MK-8(H2) and MK-10(H2) and that of P. scleromae DSM 17756T was composed of the major menaquinones MK-9(H2) and MK-8(H2) and MK-9.
31424381	7	13	dep	menaquinones	1494:1505	arg1	MK-8					1520:1523	MK-8	1520:1523	MK-8	1520:1523	The quinone system of P. phenanthrenivorans DSM 18606T was composed of the major menaquinones MK-9(H2), MK-8(H2) and MK-10(H2) and that of P. scleromae DSM 17756T was composed of the major menaquinones MK-9(H2) and MK-8(H2) and MK-9.
31424381	7	13	dep	menaquinones	1494:1505	arg1	menaquinones					1494:1505	the major menaquinones MK-9(H2) and MK-8(H2) and MK-9	1484:1536	the major menaquinones MK-9(H2) and MK-8(H2) and MK-9	1484:1536	The quinone system of P. phenanthrenivorans DSM 18606T was composed of the major menaquinones MK-9(H2), MK-8(H2) and MK-10(H2) and that of P. scleromae DSM 17756T was composed of the major menaquinones MK-9(H2) and MK-8(H2) and MK-9.
31424381	7	13	dep	menaquinones	1494:1505	arg1	MK-9					1507:1510	MK-9	1507:1510	MK-9	1507:1510	The quinone system of P. phenanthrenivorans DSM 18606T was composed of the major menaquinones MK-9(H2), MK-8(H2) and MK-10(H2) and that of P. scleromae DSM 17756T was composed of the major menaquinones MK-9(H2) and MK-8(H2) and MK-9.
31424381	8	14	theme	phosphatidylethanolamine	1605:1628	arg1	profile					1558:1564	the polar lipid profile	1542:1564	the polar lipid profile of P. phenanthrenivorans DSM 18606T no phosphatidylethanolamine	1542:1628	In the polar lipid profile of P. phenanthrenivorans DSM 18606T no phosphatidylethanolamine could be detected.
31424381	6	15	theme	phenanthrenivorans	1068:1085	arg1	profile					1054:1060	the polar lipid profile	1038:1060	the polar lipid profile of P. phenanthrenivorans	1038:1085	Since the polar lipid profile of P. phenanthrenivorans was reported to contain phosphatidylethanolamine, which is unusual for a member of the Micrococcaceae, and the polar lipid profile of P. scleromae was unknown, the polar lipids of these two species were also analysed.
31424381	7	16	theme	DSM	1457:1459	arg1	17756T					1461:1466	P. scleromae DSM 17756T	1444:1466	P. scleromae DSM 17756T	1444:1466	The quinone system of P. phenanthrenivorans DSM 18606T was composed of the major menaquinones MK-9(H2), MK-8(H2) and MK-10(H2) and that of P. scleromae DSM 17756T was composed of the major menaquinones MK-9(H2) and MK-8(H2) and MK-9.
31424381	7	17	theme	P.	1327:1328	arg1	18606T					1353:1358	P. phenanthrenivorans DSM 18606T	1327:1358	P. phenanthrenivorans DSM 18606T	1327:1358	The quinone system of P. phenanthrenivorans DSM 18606T was composed of the major menaquinones MK-9(H2), MK-8(H2) and MK-10(H2) and that of P. scleromae DSM 17756T was composed of the major menaquinones MK-9(H2) and MK-8(H2) and MK-9.
31424381	5	18	theme	genus	968:972	arg1	species					953:959	other species	947:959	other species of the genus	947:972	Since the reported quinone systems of Pseudarthrobacterphenanthrenivorans [MK-8 and MK-9(H2)] and Pseudarthrobacterscleromae [MK-8(H2] were clearly different from those of other species of the genus, the quinone systems of the two species were re-analyzed.
31424381	6	19	theme	lipid	1048:1052	arg1	profile					1054:1060	the polar lipid profile	1038:1060	the polar lipid profile of P. phenanthrenivorans	1038:1085	Since the polar lipid profile of P. phenanthrenivorans was reported to contain phosphatidylethanolamine, which is unusual for a member of the Micrococcaceae, and the polar lipid profile of P. scleromae was unknown, the polar lipids of these two species were also analysed.
31424381	5	20	theme	quinone	794:800	arg1	different					923:931	different	923:931	different	923:931	Since the reported quinone systems of Pseudarthrobacterphenanthrenivorans [MK-8 and MK-9(H2)] and Pseudarthrobacterscleromae [MK-8(H2] were clearly different from those of other species of the genus, the quinone systems of the two species were re-analyzed.
31424381	5	20	theme	quinone	794:800	arg1	systems					802:808	the reported quinone systems	781:808	the reported quinone systems of Pseudarthrobacterphenanthrenivorans [MK-8 and MK-9(H2)] and Pseudarthrobacterscleromae [MK-8(H2]	781:908	Since the reported quinone systems of Pseudarthrobacterphenanthrenivorans [MK-8 and MK-9(H2)] and Pseudarthrobacterscleromae [MK-8(H2] were clearly different from those of other species of the genus, the quinone systems of the two species were re-analyzed.
31424381	10	21	theme	Pseudarthrobacter	1806:1822	arg1	descriptions					1780:1791	the descriptions	1776:1791	the descriptions of the genus Pseudarthrobacter	1776:1822	nov. and emend the descriptions of the genus Pseudarthrobacter and the two species P. phenanthrenivorans and P. scleromae.
31424381	10	21	theme	Pseudarthrobacter	1806:1822	arg1	species					1836:1842	the two species	1828:1842	the two species P. phenanthrenivorans and P. scleromae	1828:1881	nov. and emend the descriptions of the genus Pseudarthrobacter and the two species P. phenanthrenivorans and P. scleromae.
31424381	4	22	theme	Pseudarthrobacter	756:772	arg1	characteristics					727:741	the characteristics	723:741	the characteristics of the genus Pseudarthrobacter	723:772	These data demonstrate that A. enclensis shares the characteristics of the genus Pseudarthrobacter.
31424381	8	23	theme	P.	1569:1570	arg1	phosphatidylethanolamine					1605:1628	P. phenanthrenivorans DSM 18606T no phosphatidylethanolamine	1569:1628	P. phenanthrenivorans DSM 18606T no phosphatidylethanolamine	1569:1628	In the polar lipid profile of P. phenanthrenivorans DSM 18606T no phosphatidylethanolamine could be detected.
31424381	10	24	theme	genus	1800:1804	arg1	Pseudarthrobacter					1806:1822	the genus Pseudarthrobacter	1796:1822	the genus Pseudarthrobacter	1796:1822	nov. and emend the descriptions of the genus Pseudarthrobacter and the two species P. phenanthrenivorans and P. scleromae.
31424381	6	25	theme	scleromae	1224:1232	arg1	unknown					1238:1244	unknown	1238:1244	unknown	1238:1244	Since the polar lipid profile of P. phenanthrenivorans was reported to contain phosphatidylethanolamine, which is unusual for a member of the Micrococcaceae, and the polar lipid profile of P. scleromae was unknown, the polar lipids of these two species were also analysed.
31424381	6	25	theme	scleromae	1224:1232	arg1	profile					1210:1216	the polar lipid profile	1194:1216	the polar lipid profile of P. scleromae	1194:1232	Since the polar lipid profile of P. phenanthrenivorans was reported to contain phosphatidylethanolamine, which is unusual for a member of the Micrococcaceae, and the polar lipid profile of P. scleromae was unknown, the polar lipids of these two species were also analysed.
31424381	8	26	theme	lipid	1552:1556	arg1	profile					1558:1564	the polar lipid profile	1542:1564	the polar lipid profile of P. phenanthrenivorans DSM 18606T no phosphatidylethanolamine	1542:1628	In the polar lipid profile of P. phenanthrenivorans DSM 18606T no phosphatidylethanolamine could be detected.
31424381	1	27	theme	emended	90:96	arg1	descriptions					98:109	emended descriptions	90:109	emended descriptions of the genus Pseudarthrobacter	90:140	nov., and emended descriptions of the genus Pseudarthrobacter, and the species Pseudarthrobacter phenanthrenivorans and Pseudarthrobacter scleromae.
31424381	2	28	theme	MK-8	435:438	arg1	H4					440:441	H4	440:441	H4	440:441	Arthrobacterenclensis was reported to cluster with species of the genus Pseudarthrobacter but the peptidoglycan containing lysine, alanine and glutamic acid and the presence of minor amounts of menaquinone MK-8(H4) were not in line with the description of the genus.
31424381	2	28	theme	MK-8	435:438	arg1	amounts					412:418	minor amounts	406:418	minor amounts of menaquinone MK-8(H4)	406:442	Arthrobacterenclensis was reported to cluster with species of the genus Pseudarthrobacter but the peptidoglycan containing lysine, alanine and glutamic acid and the presence of minor amounts of menaquinone MK-8(H4) were not in line with the description of the genus.
31424381	2	28	theme	MK-8	435:438	arg1	MK-8					435:438	menaquinone MK-8	423:438	menaquinone MK-8(H4)	423:442	Arthrobacterenclensis was reported to cluster with species of the genus Pseudarthrobacter but the peptidoglycan containing lysine, alanine and glutamic acid and the presence of minor amounts of menaquinone MK-8(H4) were not in line with the description of the genus.
31424381	1	29	dep	phenanthrenivorans	177:194	arg1	descriptions					98:109	emended descriptions	90:109	emended descriptions of the genus Pseudarthrobacter	90:140	nov., and emended descriptions of the genus Pseudarthrobacter, and the species Pseudarthrobacter phenanthrenivorans and Pseudarthrobacter scleromae.
31424381	1	29	dep	phenanthrenivorans	177:194	arg1	nov.					80:83	nov.	80:83	nov.	80:83	nov., and emended descriptions of the genus Pseudarthrobacter, and the species Pseudarthrobacter phenanthrenivorans and Pseudarthrobacter scleromae.
31424381	1	29	dep	phenanthrenivorans	177:194	arg1	species					151:157	the species	147:157	the species	147:157	nov., and emended descriptions of the genus Pseudarthrobacter, and the species Pseudarthrobacter phenanthrenivorans and Pseudarthrobacter scleromae.
31424381	8	30	theme	DSM	1591:1593	arg1	phosphatidylethanolamine					1605:1628	P. phenanthrenivorans DSM 18606T no phosphatidylethanolamine	1569:1628	P. phenanthrenivorans DSM 18606T no phosphatidylethanolamine	1569:1628	In the polar lipid profile of P. phenanthrenivorans DSM 18606T no phosphatidylethanolamine could be detected.
31424381	2	31	theme	menaquinone	423:433	arg1	H4					440:441	H4	440:441	H4	440:441	Arthrobacterenclensis was reported to cluster with species of the genus Pseudarthrobacter but the peptidoglycan containing lysine, alanine and glutamic acid and the presence of minor amounts of menaquinone MK-8(H4) were not in line with the description of the genus.
31424381	2	31	theme	menaquinone	423:433	arg1	MK-8					435:438	menaquinone MK-8	423:438	menaquinone MK-8(H4)	423:442	Arthrobacterenclensis was reported to cluster with species of the genus Pseudarthrobacter but the peptidoglycan containing lysine, alanine and glutamic acid and the presence of minor amounts of menaquinone MK-8(H4) were not in line with the description of the genus.
31424381	0	32	theme	enclensis	33:41	arg1	Reclassification					0:15	Reclassification	0:15	Reclassification of Arthrobacter enclensis as Pseudarthrobacter enclensis comb.	0:78	Reclassification of Arthrobacter enclensis as Pseudarthrobacter enclensis comb.
31424381	2	33	with	cluster	267:273	arg1	species					280:286	species	280:286	species of the genus Pseudarthrobacter	280:317	Arthrobacterenclensis was reported to cluster with species of the genus Pseudarthrobacter but the peptidoglycan containing lysine, alanine and glutamic acid and the presence of minor amounts of menaquinone MK-8(H4) were not in line with the description of the genus.
31424381	6	34	theme	polar	1198:1202	arg1	unknown					1238:1244	unknown	1238:1244	unknown	1238:1244	Since the polar lipid profile of P. phenanthrenivorans was reported to contain phosphatidylethanolamine, which is unusual for a member of the Micrococcaceae, and the polar lipid profile of P. scleromae was unknown, the polar lipids of these two species were also analysed.
31424381	6	34	theme	polar	1198:1202	arg1	profile					1210:1216	the polar lipid profile	1194:1216	the polar lipid profile of P. scleromae	1194:1232	Since the polar lipid profile of P. phenanthrenivorans was reported to contain phosphatidylethanolamine, which is unusual for a member of the Micrococcaceae, and the polar lipid profile of P. scleromae was unknown, the polar lipids of these two species were also analysed.
31424381	5	35	theme	other	947:951	arg1	species					953:959	other species	947:959	other species of the genus	947:972	Since the reported quinone systems of Pseudarthrobacterphenanthrenivorans [MK-8 and MK-9(H2)] and Pseudarthrobacterscleromae [MK-8(H2] were clearly different from those of other species of the genus, the quinone systems of the two species were re-analyzed.
31424381	8	36	theme	phenanthrenivorans	1572:1589	arg1	phosphatidylethanolamine					1605:1628	P. phenanthrenivorans DSM 18606T no phosphatidylethanolamine	1569:1628	P. phenanthrenivorans DSM 18606T no phosphatidylethanolamine	1569:1628	In the polar lipid profile of P. phenanthrenivorans DSM 18606T no phosphatidylethanolamine could be detected.
31424381	0	37	theme	Arthrobacter	20:31	arg1	enclensis					33:41	Arthrobacter enclensis	20:41	Arthrobacter enclensis	20:41	Reclassification of Arthrobacter enclensis as Pseudarthrobacter enclensis comb.
31424381	2	38	contain	containing	341:350	arg2	presence					394:401	the presence	390:401	the presence of minor amounts of menaquinone MK-8(H4)	390:442	Arthrobacterenclensis was reported to cluster with species of the genus Pseudarthrobacter but the peptidoglycan containing lysine, alanine and glutamic acid and the presence of minor amounts of menaquinone MK-8(H4) were not in line with the description of the genus.
31424381	2	38	contain	containing	341:350	arg2	alanine					360:366	alanine	360:366	alanine	360:366	Arthrobacterenclensis was reported to cluster with species of the genus Pseudarthrobacter but the peptidoglycan containing lysine, alanine and glutamic acid and the presence of minor amounts of menaquinone MK-8(H4) were not in line with the description of the genus.
31424381	2	38	contain	containing	341:350	arg2	lysine					352:357	lysine	352:357	lysine	352:357	Arthrobacterenclensis was reported to cluster with species of the genus Pseudarthrobacter but the peptidoglycan containing lysine, alanine and glutamic acid and the presence of minor amounts of menaquinone MK-8(H4) were not in line with the description of the genus.
31424381	2	38	contain	containing	341:350	arg1	peptidoglycan					327:339	the peptidoglycan	323:339	the peptidoglycan containing lysine, alanine and glutamic acid and the presence of minor amounts of menaquinone MK-8(H4)	323:442	Arthrobacterenclensis was reported to cluster with species of the genus Pseudarthrobacter but the peptidoglycan containing lysine, alanine and glutamic acid and the presence of minor amounts of menaquinone MK-8(H4) were not in line with the description of the genus.
31424381	7	39	theme	major	1380:1384	arg1	menaquinones					1386:1397	the major menaquinones MK-9(H2), MK-8(H2) and MK-10(H2)	1376:1430	the major menaquinones MK-9(H2), MK-8(H2) and MK-10(H2)	1376:1430	The quinone system of P. phenanthrenivorans DSM 18606T was composed of the major menaquinones MK-9(H2), MK-8(H2) and MK-10(H2) and that of P. scleromae DSM 17756T was composed of the major menaquinones MK-9(H2) and MK-8(H2) and MK-9.
31424381	7	39	theme	major	1380:1384	arg1	MK-10					1422:1426	MK-10	1422:1426	MK-10	1422:1426	The quinone system of P. phenanthrenivorans DSM 18606T was composed of the major menaquinones MK-9(H2), MK-8(H2) and MK-10(H2) and that of P. scleromae DSM 17756T was composed of the major menaquinones MK-9(H2) and MK-8(H2) and MK-9.
31424381	7	39	theme	major	1380:1384	arg1	MK-8					1409:1412	MK-8	1409:1412	MK-8	1409:1412	The quinone system of P. phenanthrenivorans DSM 18606T was composed of the major menaquinones MK-9(H2), MK-8(H2) and MK-10(H2) and that of P. scleromae DSM 17756T was composed of the major menaquinones MK-9(H2) and MK-8(H2) and MK-9.
31424381	7	39	theme	major	1380:1384	arg1	MK-9					1399:1402	MK-9	1399:1402	MK-9	1399:1402	The quinone system of P. phenanthrenivorans DSM 18606T was composed of the major menaquinones MK-9(H2), MK-8(H2) and MK-10(H2) and that of P. scleromae DSM 17756T was composed of the major menaquinones MK-9(H2) and MK-8(H2) and MK-9.
31424381	3	40	theme	quinone	632:638	arg1	system					640:645	the quinone system	628:645	the quinone system of A. enclensis DSM 25279T	628:672	Re-analysis of these traits revealed a peptidoglycan with l-Lys-l-Ser-l-Thr-l-Ala and no MK-8(H4), but major amounts of MK-9(H2) in the quinone system of A. enclensis DSM 25279T.
31424381	8	41	theme	18606T	1595:1600	arg1	phosphatidylethanolamine					1605:1628	P. phenanthrenivorans DSM 18606T no phosphatidylethanolamine	1569:1628	P. phenanthrenivorans DSM 18606T no phosphatidylethanolamine	1569:1628	In the polar lipid profile of P. phenanthrenivorans DSM 18606T no phosphatidylethanolamine could be detected.
31424381	8	42	theme	no	1602:1603	arg1	phosphatidylethanolamine					1605:1628	P. phenanthrenivorans DSM 18606T no phosphatidylethanolamine	1569:1628	P. phenanthrenivorans DSM 18606T no phosphatidylethanolamine	1569:1628	In the polar lipid profile of P. phenanthrenivorans DSM 18606T no phosphatidylethanolamine could be detected.
31424381	5	43	theme	MK-9	859:862	arg1	different					923:931	different	923:931	different	923:931	Since the reported quinone systems of Pseudarthrobacterphenanthrenivorans [MK-8 and MK-9(H2)] and Pseudarthrobacterscleromae [MK-8(H2] were clearly different from those of other species of the genus, the quinone systems of the two species were re-analyzed.
31424381	5	43	theme	MK-9	859:862	arg1	systems					802:808	the reported quinone systems	781:808	the reported quinone systems of Pseudarthrobacterphenanthrenivorans [MK-8 and MK-9(H2)] and Pseudarthrobacterscleromae [MK-8(H2]	781:908	Since the reported quinone systems of Pseudarthrobacterphenanthrenivorans [MK-8 and MK-9(H2)] and Pseudarthrobacterscleromae [MK-8(H2] were clearly different from those of other species of the genus, the quinone systems of the two species were re-analyzed.
31424381	0	44	theme	Pseudarthrobacter	46:62	arg1	comb					74:77	Pseudarthrobacter enclensis comb	46:77	Pseudarthrobacter enclensis comb	46:77	Reclassification of Arthrobacter enclensis as Pseudarthrobacter enclensis comb.
31424381	3	45	theme	traits	517:522	arg1	Re-analysis					496:506	Re-analysis	496:506	Re-analysis of these traits	496:522	Re-analysis of these traits revealed a peptidoglycan with l-Lys-l-Ser-l-Thr-l-Ala and no MK-8(H4), but major amounts of MK-9(H2) in the quinone system of A. enclensis DSM 25279T.
31424381	6	46	contain	contain	1103:1109	arg2	phosphatidylethanolamine					1111:1134	phosphatidylethanolamine	1111:1134	phosphatidylethanolamine	1111:1134	Since the polar lipid profile of P. phenanthrenivorans was reported to contain phosphatidylethanolamine, which is unusual for a member of the Micrococcaceae, and the polar lipid profile of P. scleromae was unknown, the polar lipids of these two species were also analysed.
31424381	6	46	contain	contain	1103:1109	arg1	profile					1054:1060	the polar lipid profile	1038:1060	the polar lipid profile of P. phenanthrenivorans	1038:1085	Since the polar lipid profile of P. phenanthrenivorans was reported to contain phosphatidylethanolamine, which is unusual for a member of the Micrococcaceae, and the polar lipid profile of P. scleromae was unknown, the polar lipids of these two species were also analysed.
31424381	7	47	theme	DSM	1349:1351	arg1	18606T					1353:1358	P. phenanthrenivorans DSM 18606T	1327:1358	P. phenanthrenivorans DSM 18606T	1327:1358	The quinone system of P. phenanthrenivorans DSM 18606T was composed of the major menaquinones MK-9(H2), MK-8(H2) and MK-10(H2) and that of P. scleromae DSM 17756T was composed of the major menaquinones MK-9(H2) and MK-8(H2) and MK-9.
31424381	5	48	theme	Pseudarthrobacterscleromae	873:898	arg1	different					923:931	different	923:931	different	923:931	Since the reported quinone systems of Pseudarthrobacterphenanthrenivorans [MK-8 and MK-9(H2)] and Pseudarthrobacterscleromae [MK-8(H2] were clearly different from those of other species of the genus, the quinone systems of the two species were re-analyzed.
31424381	5	48	theme	Pseudarthrobacterscleromae	873:898	arg1	systems					802:808	the reported quinone systems	781:808	the reported quinone systems of Pseudarthrobacterphenanthrenivorans [MK-8 and MK-9(H2)] and Pseudarthrobacterscleromae [MK-8(H2]	781:908	Since the reported quinone systems of Pseudarthrobacterphenanthrenivorans [MK-8 and MK-9(H2)] and Pseudarthrobacterscleromae [MK-8(H2] were clearly different from those of other species of the genus, the quinone systems of the two species were re-analyzed.
31424381	2	49	theme	genus	489:493	arg1	description					470:480	the description	466:480	the description of the genus	466:493	Arthrobacterenclensis was reported to cluster with species of the genus Pseudarthrobacter but the peptidoglycan containing lysine, alanine and glutamic acid and the presence of minor amounts of menaquinone MK-8(H4) were not in line with the description of the genus.
31424381	5	50	theme	Pseudarthrobacterphenanthrenivorans	813:847	arg1	different					923:931	different	923:931	different	923:931	Since the reported quinone systems of Pseudarthrobacterphenanthrenivorans [MK-8 and MK-9(H2)] and Pseudarthrobacterscleromae [MK-8(H2] were clearly different from those of other species of the genus, the quinone systems of the two species were re-analyzed.
31424381	5	50	theme	Pseudarthrobacterphenanthrenivorans	813:847	arg1	systems					802:808	the reported quinone systems	781:808	the reported quinone systems of Pseudarthrobacterphenanthrenivorans [MK-8 and MK-9(H2)] and Pseudarthrobacterscleromae [MK-8(H2]	781:908	Since the reported quinone systems of Pseudarthrobacterphenanthrenivorans [MK-8 and MK-9(H2)] and Pseudarthrobacterscleromae [MK-8(H2] were clearly different from those of other species of the genus, the quinone systems of the two species were re-analyzed.
31424381	7	51	theme	phenanthrenivorans	1330:1347	arg1	18606T					1353:1358	P. phenanthrenivorans DSM 18606T	1327:1358	P. phenanthrenivorans DSM 18606T	1327:1358	The quinone system of P. phenanthrenivorans DSM 18606T was composed of the major menaquinones MK-9(H2), MK-8(H2) and MK-10(H2) and that of P. scleromae DSM 17756T was composed of the major menaquinones MK-9(H2) and MK-8(H2) and MK-9.
31424381	5	52	theme	reported	785:792	arg1	different					923:931	different	923:931	different	923:931	Since the reported quinone systems of Pseudarthrobacterphenanthrenivorans [MK-8 and MK-9(H2)] and Pseudarthrobacterscleromae [MK-8(H2] were clearly different from those of other species of the genus, the quinone systems of the two species were re-analyzed.
31424381	5	52	theme	reported	785:792	arg1	systems					802:808	the reported quinone systems	781:808	the reported quinone systems of Pseudarthrobacterphenanthrenivorans [MK-8 and MK-9(H2)] and Pseudarthrobacterscleromae [MK-8(H2]	781:908	Since the reported quinone systems of Pseudarthrobacterphenanthrenivorans [MK-8 and MK-9(H2)] and Pseudarthrobacterscleromae [MK-8(H2] were clearly different from those of other species of the genus, the quinone systems of the two species were re-analyzed.
31424381	6	53	theme	species	1277:1283	arg1	lipids					1257:1262	the polar lipids	1247:1262	the polar lipids of these two species	1247:1283	Since the polar lipid profile of P. phenanthrenivorans was reported to contain phosphatidylethanolamine, which is unusual for a member of the Micrococcaceae, and the polar lipid profile of P. scleromae was unknown, the polar lipids of these two species were also analysed.
31424381	7	54	dep	menaquinones	1386:1397	arg1	H2					1404:1405	H2	1404:1405	H2	1404:1405	The quinone system of P. phenanthrenivorans DSM 18606T was composed of the major menaquinones MK-9(H2), MK-8(H2) and MK-10(H2) and that of P. scleromae DSM 17756T was composed of the major menaquinones MK-9(H2) and MK-8(H2) and MK-9.
31424381	7	54	dep	menaquinones	1386:1397	arg1	MK-10					1422:1426	MK-10	1422:1426	MK-10	1422:1426	The quinone system of P. phenanthrenivorans DSM 18606T was composed of the major menaquinones MK-9(H2), MK-8(H2) and MK-10(H2) and that of P. scleromae DSM 17756T was composed of the major menaquinones MK-9(H2) and MK-8(H2) and MK-9.
31424381	7	54	dep	menaquinones	1386:1397	arg1	MK-8					1409:1412	MK-8	1409:1412	MK-8	1409:1412	The quinone system of P. phenanthrenivorans DSM 18606T was composed of the major menaquinones MK-9(H2), MK-8(H2) and MK-10(H2) and that of P. scleromae DSM 17756T was composed of the major menaquinones MK-9(H2) and MK-8(H2) and MK-9.
31424381	7	54	dep	menaquinones	1386:1397	arg1	H2					1428:1429	H2	1428:1429	H2	1428:1429	The quinone system of P. phenanthrenivorans DSM 18606T was composed of the major menaquinones MK-9(H2), MK-8(H2) and MK-10(H2) and that of P. scleromae DSM 17756T was composed of the major menaquinones MK-9(H2) and MK-8(H2) and MK-9.
31424381	7	54	dep	menaquinones	1386:1397	arg1	menaquinones					1386:1397	the major menaquinones MK-9(H2), MK-8(H2) and MK-10(H2)	1376:1430	the major menaquinones MK-9(H2), MK-8(H2) and MK-10(H2)	1376:1430	The quinone system of P. phenanthrenivorans DSM 18606T was composed of the major menaquinones MK-9(H2), MK-8(H2) and MK-10(H2) and that of P. scleromae DSM 17756T was composed of the major menaquinones MK-9(H2) and MK-8(H2) and MK-9.
31424381	7	54	dep	menaquinones	1386:1397	arg1	MK-9					1399:1402	MK-9	1399:1402	MK-9	1399:1402	The quinone system of P. phenanthrenivorans DSM 18606T was composed of the major menaquinones MK-9(H2), MK-8(H2) and MK-10(H2) and that of P. scleromae DSM 17756T was composed of the major menaquinones MK-9(H2) and MK-8(H2) and MK-9.
31424381	7	54	dep	menaquinones	1386:1397	arg1	H2					1414:1415	H2	1414:1415	H2	1414:1415	The quinone system of P. phenanthrenivorans DSM 18606T was composed of the major menaquinones MK-9(H2), MK-8(H2) and MK-10(H2) and that of P. scleromae DSM 17756T was composed of the major menaquinones MK-9(H2) and MK-8(H2) and MK-9.
31424381	5	55	theme	species	1006:1012	arg1	systems					987:993	the quinone systems	975:993	the quinone systems of the two species	975:1012	Since the reported quinone systems of Pseudarthrobacterphenanthrenivorans [MK-8 and MK-9(H2)] and Pseudarthrobacterscleromae [MK-8(H2] were clearly different from those of other species of the genus, the quinone systems of the two species were re-analyzed.
31424381	2	56	with	line	456:459	arg1	description					470:480	the description	466:480	the description of the genus	466:493	Arthrobacterenclensis was reported to cluster with species of the genus Pseudarthrobacter but the peptidoglycan containing lysine, alanine and glutamic acid and the presence of minor amounts of menaquinone MK-8(H4) were not in line with the description of the genus.
31424381	1	57	theme	genus	118:122	arg1	Pseudarthrobacter					124:140	the genus Pseudarthrobacter	114:140	the genus Pseudarthrobacter	114:140	nov., and emended descriptions of the genus Pseudarthrobacter, and the species Pseudarthrobacter phenanthrenivorans and Pseudarthrobacter scleromae.
31424381	0	58	theme	enclensis	64:72	arg1	comb					74:77	Pseudarthrobacter enclensis comb	46:77	Pseudarthrobacter enclensis comb	46:77	Reclassification of Arthrobacter enclensis as Pseudarthrobacter enclensis comb.
31424381	6	59	theme	polar	1042:1046	arg1	profile					1054:1060	the polar lipid profile	1038:1060	the polar lipid profile of P. phenanthrenivorans	1038:1085	Since the polar lipid profile of P. phenanthrenivorans was reported to contain phosphatidylethanolamine, which is unusual for a member of the Micrococcaceae, and the polar lipid profile of P. scleromae was unknown, the polar lipids of these two species were also analysed.
31424381	3	60	theme	enclensis	653:661	arg1	25279T					667:672	A. enclensis DSM 25279T	650:672	A. enclensis DSM 25279T	650:672	Re-analysis of these traits revealed a peptidoglycan with l-Lys-l-Ser-l-Thr-l-Ala and no MK-8(H4), but major amounts of MK-9(H2) in the quinone system of A. enclensis DSM 25279T.
31424381	3	61	theme	major	599:603	arg1	amounts					605:611	major amounts	599:611	major amounts of MK-9(H2)	599:623	Re-analysis of these traits revealed a peptidoglycan with l-Lys-l-Ser-l-Thr-l-Ala and no MK-8(H4), but major amounts of MK-9(H2) in the quinone system of A. enclensis DSM 25279T.
31424381	3	61	theme	major	599:603	arg1	H2					621:622	H2	621:622	H2	621:622	Re-analysis of these traits revealed a peptidoglycan with l-Lys-l-Ser-l-Thr-l-Ala and no MK-8(H4), but major amounts of MK-9(H2) in the quinone system of A. enclensis DSM 25279T.
31424381	3	61	theme	major	599:603	arg1	MK-9					616:619	MK-9	616:619	MK-9	616:619	Re-analysis of these traits revealed a peptidoglycan with l-Lys-l-Ser-l-Thr-l-Ala and no MK-8(H4), but major amounts of MK-9(H2) in the quinone system of A. enclensis DSM 25279T.
31424381	1	62	theme	Pseudarthrobacter	124:140	arg1	species					151:157	the species	147:157	the species	147:157	nov., and emended descriptions of the genus Pseudarthrobacter, and the species Pseudarthrobacter phenanthrenivorans and Pseudarthrobacter scleromae.
31424381	1	62	theme	Pseudarthrobacter	124:140	arg1	nov.					80:83	nov.	80:83	nov.	80:83	nov., and emended descriptions of the genus Pseudarthrobacter, and the species Pseudarthrobacter phenanthrenivorans and Pseudarthrobacter scleromae.
31424381	1	62	theme	Pseudarthrobacter	124:140	arg1	descriptions					98:109	emended descriptions	90:109	emended descriptions of the genus Pseudarthrobacter	90:140	nov., and emended descriptions of the genus Pseudarthrobacter, and the species Pseudarthrobacter phenanthrenivorans and Pseudarthrobacter scleromae.
31424381	7	63	theme	P.	1444:1445	arg1	17756T					1461:1466	P. scleromae DSM 17756T	1444:1466	P. scleromae DSM 17756T	1444:1466	The quinone system of P. phenanthrenivorans DSM 18606T was composed of the major menaquinones MK-9(H2), MK-8(H2) and MK-10(H2) and that of P. scleromae DSM 17756T was composed of the major menaquinones MK-9(H2) and MK-8(H2) and MK-9.
31424381	7	64	theme	quinone	1309:1315	arg1	system					1317:1322	The quinone system	1305:1322	The quinone system of P. phenanthrenivorans DSM 18606T	1305:1358	The quinone system of P. phenanthrenivorans DSM 18606T was composed of the major menaquinones MK-9(H2), MK-8(H2) and MK-10(H2) and that of P. scleromae DSM 17756T was composed of the major menaquinones MK-9(H2) and MK-8(H2) and MK-9.
31424381	3	65	theme	DSM	663:665	arg1	25279T					667:672	A. enclensis DSM 25279T	650:672	A. enclensis DSM 25279T	650:672	Re-analysis of these traits revealed a peptidoglycan with l-Lys-l-Ser-l-Thr-l-Ala and no MK-8(H4), but major amounts of MK-9(H2) in the quinone system of A. enclensis DSM 25279T.
31424381	7	66	theme	18606T	1353:1358	arg1	system					1317:1322	The quinone system	1305:1322	The quinone system of P. phenanthrenivorans DSM 18606T	1305:1358	The quinone system of P. phenanthrenivorans DSM 18606T was composed of the major menaquinones MK-9(H2), MK-8(H2) and MK-10(H2) and that of P. scleromae DSM 17756T was composed of the major menaquinones MK-9(H2) and MK-8(H2) and MK-9.
31424381	3	67	theme	MK-9	616:619	arg1	amounts					605:611	major amounts	599:611	major amounts of MK-9(H2)	599:623	Re-analysis of these traits revealed a peptidoglycan with l-Lys-l-Ser-l-Thr-l-Ala and no MK-8(H4), but major amounts of MK-9(H2) in the quinone system of A. enclensis DSM 25279T.
31424381	3	67	theme	MK-9	616:619	arg1	H2					621:622	H2	621:622	H2	621:622	Re-analysis of these traits revealed a peptidoglycan with l-Lys-l-Ser-l-Thr-l-Ala and no MK-8(H4), but major amounts of MK-9(H2) in the quinone system of A. enclensis DSM 25279T.
31424381	3	67	theme	MK-9	616:619	arg1	MK-9					616:619	MK-9	616:619	MK-9	616:619	Re-analysis of these traits revealed a peptidoglycan with l-Lys-l-Ser-l-Thr-l-Ala and no MK-8(H4), but major amounts of MK-9(H2) in the quinone system of A. enclensis DSM 25279T.
31424381	2	68	theme	Pseudarthrobacter	301:317	arg1	species					280:286	species	280:286	species of the genus Pseudarthrobacter	280:317	Arthrobacterenclensis was reported to cluster with species of the genus Pseudarthrobacter but the peptidoglycan containing lysine, alanine and glutamic acid and the presence of minor amounts of menaquinone MK-8(H4) were not in line with the description of the genus.
31424381	5	69	from	those	938:942	arg1	different					923:931	different	923:931	different	923:931	Since the reported quinone systems of Pseudarthrobacterphenanthrenivorans [MK-8 and MK-9(H2)] and Pseudarthrobacterscleromae [MK-8(H2] were clearly different from those of other species of the genus, the quinone systems of the two species were re-analyzed.
31424381	5	69	from	those	938:942	arg1	systems					802:808	the reported quinone systems	781:808	the reported quinone systems of Pseudarthrobacterphenanthrenivorans [MK-8 and MK-9(H2)] and Pseudarthrobacterscleromae [MK-8(H2]	781:908	Since the reported quinone systems of Pseudarthrobacterphenanthrenivorans [MK-8 and MK-9(H2)] and Pseudarthrobacterscleromae [MK-8(H2] were clearly different from those of other species of the genus, the quinone systems of the two species were re-analyzed.
31424381	7	70	theme	major	1488:1492	arg1	menaquinones					1494:1505	the major menaquinones MK-9(H2) and MK-8(H2) and MK-9	1484:1536	the major menaquinones MK-9(H2) and MK-8(H2) and MK-9	1484:1536	The quinone system of P. phenanthrenivorans DSM 18606T was composed of the major menaquinones MK-9(H2), MK-8(H2) and MK-10(H2) and that of P. scleromae DSM 17756T was composed of the major menaquinones MK-9(H2) and MK-8(H2) and MK-9.
31424381	7	70	theme	major	1488:1492	arg1	MK-9					1533:1536	MK-9	1533:1536	MK-9	1533:1536	The quinone system of P. phenanthrenivorans DSM 18606T was composed of the major menaquinones MK-9(H2), MK-8(H2) and MK-10(H2) and that of P. scleromae DSM 17756T was composed of the major menaquinones MK-9(H2) and MK-8(H2) and MK-9.
31424381	7	70	theme	major	1488:1492	arg1	MK-8					1520:1523	MK-8	1520:1523	MK-8	1520:1523	The quinone system of P. phenanthrenivorans DSM 18606T was composed of the major menaquinones MK-9(H2), MK-8(H2) and MK-10(H2) and that of P. scleromae DSM 17756T was composed of the major menaquinones MK-9(H2) and MK-8(H2) and MK-9.
31424381	7	70	theme	major	1488:1492	arg1	MK-9					1507:1510	MK-9	1507:1510	MK-9	1507:1510	The quinone system of P. phenanthrenivorans DSM 18606T was composed of the major menaquinones MK-9(H2), MK-8(H2) and MK-10(H2) and that of P. scleromae DSM 17756T was composed of the major menaquinones MK-9(H2) and MK-8(H2) and MK-9.
31424381	3	71	theme	A.	650:651	arg1	25279T					667:672	A. enclensis DSM 25279T	650:672	A. enclensis DSM 25279T	650:672	Re-analysis of these traits revealed a peptidoglycan with l-Lys-l-Ser-l-Thr-l-Ala and no MK-8(H4), but major amounts of MK-9(H2) in the quinone system of A. enclensis DSM 25279T.
31424381	2	72	theme	genus	295:299	arg1	Pseudarthrobacter					301:317	the genus Pseudarthrobacter	291:317	the genus Pseudarthrobacter	291:317	Arthrobacterenclensis was reported to cluster with species of the genus Pseudarthrobacter but the peptidoglycan containing lysine, alanine and glutamic acid and the presence of minor amounts of menaquinone MK-8(H4) were not in line with the description of the genus.
31424381	6	73	theme	Micrococcaceae	1174:1187	arg1	member					1160:1165	a member	1158:1165	a member of the Micrococcaceae	1158:1187	Since the polar lipid profile of P. phenanthrenivorans was reported to contain phosphatidylethanolamine, which is unusual for a member of the Micrococcaceae, and the polar lipid profile of P. scleromae was unknown, the polar lipids of these two species were also analysed.
31424381	9	74	theme	enclensis	1715:1723	arg1	reclassification					1692:1707	the reclassification	1688:1707	the reclassification of A. enclensis	1688:1723	Based on these results we here propose the reclassification of A. enclensis as Pseudarthrobacterenclensis comb.
31424381	9	74	theme	enclensis	1715:1723	arg1	comb					1755:1758	Pseudarthrobacterenclensis comb	1728:1758	Pseudarthrobacterenclensis comb	1728:1758	Based on these results we here propose the reclassification of A. enclensis as Pseudarthrobacterenclensis comb.
31424381	2	75	theme	amounts	412:418	arg1	alanine					360:366	alanine	360:366	alanine	360:366	Arthrobacterenclensis was reported to cluster with species of the genus Pseudarthrobacter but the peptidoglycan containing lysine, alanine and glutamic acid and the presence of minor amounts of menaquinone MK-8(H4) were not in line with the description of the genus.
31424381	2	75	theme	amounts	412:418	arg1	acid					381:384	glutamic acid	372:384	glutamic acid	372:384	Arthrobacterenclensis was reported to cluster with species of the genus Pseudarthrobacter but the peptidoglycan containing lysine, alanine and glutamic acid and the presence of minor amounts of menaquinone MK-8(H4) were not in line with the description of the genus.
31424381	2	75	theme	amounts	412:418	arg1	lysine					352:357	lysine	352:357	lysine	352:357	Arthrobacterenclensis was reported to cluster with species of the genus Pseudarthrobacter but the peptidoglycan containing lysine, alanine and glutamic acid and the presence of minor amounts of menaquinone MK-8(H4) were not in line with the description of the genus.
31424381	2	75	theme	amounts	412:418	arg1	presence					394:401	the presence	390:401	the presence of minor amounts of menaquinone MK-8(H4)	390:442	Arthrobacterenclensis was reported to cluster with species of the genus Pseudarthrobacter but the peptidoglycan containing lysine, alanine and glutamic acid and the presence of minor amounts of menaquinone MK-8(H4) were not in line with the description of the genus.
31715227	0	0	theme	multi-wall	91:100	arg1	carbon					102:107	magnetic multi-wall carbon	82:107	magnetic multi-wall carbon	82:107	Adsorptive removal of endocrine disrupting compounds from aqueous solutions using magnetic multi-wall carbon nanotubes modified with chitosan biopolymer based on response surface methodology: Functionalization, kinetics, and isotherms studies.
31715227	1	1	theme	ubiquitous	348:357	arg1	problem					359:365	a global and ubiquitous problem	335:365	a global and ubiquitous problem	335:365	Recently, the presence of endocrine disrupting compounds in the environment has emerged as a global and ubiquitous problem.
31715227	7	2	theme	optimum	974:980	arg1	conditions					993:1002	optimum adsorption conditions	974:1002	optimum adsorption conditions	974:1002	The results of our experiments showed that optimum adsorption conditions was achieved at t = 76 min, BPA concentration 6.5 mg/L, adsorbent dosage 1 g/L and pH = 6.2.
31715227	7	3	theme	concentration	1036:1048	arg1	6.5 mg/L					1050:1057	BPA concentration 6.5 mg/L	1032:1057	BPA concentration 6.5 mg/L	1032:1057	The results of our experiments showed that optimum adsorption conditions was achieved at t = 76 min, BPA concentration 6.5 mg/L, adsorbent dosage 1 g/L and pH = 6.2.
31715227	0	4	theme	magnetic	82:89	arg1	carbon					102:107	magnetic multi-wall carbon	82:107	magnetic multi-wall carbon	82:107	Adsorptive removal of endocrine disrupting compounds from aqueous solutions using magnetic multi-wall carbon nanotubes modified with chitosan biopolymer based on response surface methodology: Functionalization, kinetics, and isotherms studies.
31715227	6	5	theme	increased	812:820	arg1	size					839:842	increased carbon nano tube size	812:842	increased carbon nano tube size	812:842	The surface morphology of nanocomposite was observed, increased carbon nano tube size, and the levels after surface deposition were completely covered by chitosan proteins.
31715227	6	6	theme	nanocomposite	784:796	arg1	morphology					770:779	The surface morphology	758:779	The surface morphology of nanocomposite	758:796	The surface morphology of nanocomposite was observed, increased carbon nano tube size, and the levels after surface deposition were completely covered by chitosan proteins.
31715227	1	7	theme	endocrine	270:278	arg1	compounds					291:299	endocrine disrupting compounds	270:299	endocrine disrupting compounds	270:299	Recently, the presence of endocrine disrupting compounds in the environment has emerged as a global and ubiquitous problem.
31715227	1	8	theme	disrupting	280:289	arg1	compounds					291:299	endocrine disrupting compounds	270:299	endocrine disrupting compounds	270:299	Recently, the presence of endocrine disrupting compounds in the environment has emerged as a global and ubiquitous problem.
31715227	8	9	theme	isotherm	1150:1157	arg1	model					1159:1163	the Langmuir isotherm model	1137:1163	the Langmuir isotherm model	1137:1163	The data obtained in this study followed the Langmuir isotherm model and the pseudo-second order model.
31715227	1	10	theme	compounds	291:299	arg1	presence					258:265	the presence	254:265	the presence of endocrine disrupting compounds in the environment	254:318	Recently, the presence of endocrine disrupting compounds in the environment has emerged as a global and ubiquitous problem.
31715227	0	11	dep	nanotubes	109:117	arg1	kinetics					211:218	kinetics	211:218	kinetics	211:218	Adsorptive removal of endocrine disrupting compounds from aqueous solutions using magnetic multi-wall carbon nanotubes modified with chitosan biopolymer based on response surface methodology: Functionalization, kinetics, and isotherms studies.
31715227	0	11	dep	nanotubes	109:117	arg1	isotherms					225:233	isotherms	225:233	isotherms	225:233	Adsorptive removal of endocrine disrupting compounds from aqueous solutions using magnetic multi-wall carbon nanotubes modified with chitosan biopolymer based on response surface methodology: Functionalization, kinetics, and isotherms studies.
31715227	0	11	dep	nanotubes	109:117	arg1	Functionalization					192:208	Functionalization	192:208	Functionalization	192:208	Adsorptive removal of endocrine disrupting compounds from aqueous solutions using magnetic multi-wall carbon nanotubes modified with chitosan biopolymer based on response surface methodology: Functionalization, kinetics, and isotherms studies.
31715227	0	11	dep	nanotubes	109:117	arg1	modified					119:126	modified	119:126	nanotubes modified with chitosan biopolymer based on response surface methodology	109:189	Adsorptive removal of endocrine disrupting compounds from aqueous solutions using magnetic multi-wall carbon nanotubes modified with chitosan biopolymer based on response surface methodology: Functionalization, kinetics, and isotherms studies.
31715227	6	12	theme	surface	866:872	arg1	deposition					874:883	surface deposition	866:883	surface deposition	866:883	The surface morphology of nanocomposite was observed, increased carbon nano tube size, and the levels after surface deposition were completely covered by chitosan proteins.
31715227	3	13	from	effect	499:504	arg1	adsorption					555:564	the Bisphenol A (BPA) adsorption	533:564	the Bisphenol A (BPA) adsorption	533:564	The effect of different parameters on the Bisphenol A (BPA) adsorption was studied.
31715227	8	14	theme	pseudo-second	1173:1185	arg1	model					1193:1197	the pseudo-second order model	1169:1197	the pseudo-second order model	1169:1197	The data obtained in this study followed the Langmuir isotherm model and the pseudo-second order model.
31715227	8	15	theme	order	1187:1191	arg1	model					1193:1197	the pseudo-second order model	1169:1197	the pseudo-second order model	1169:1197	The data obtained in this study followed the Langmuir isotherm model and the pseudo-second order model.
31715227	9	16	theme	maximum	1204:1210	arg1	capacity					1233:1240	The maximum monolayer adsorption capacity	1200:1240	The maximum monolayer adsorption capacity of nanocomposite for BPA	1200:1265	The maximum monolayer adsorption capacity of nanocomposite for BPA was 46.2 mg/g at 20 °C.
31715227	1	17	from	presence	258:265	arg1	environment					308:318	the environment	304:318	the environment	304:318	Recently, the presence of endocrine disrupting compounds in the environment has emerged as a global and ubiquitous problem.
31715227	2	18	theme	novel	385:389	arg1	synthesis					391:399	a novel synthesis	383:399	a novel synthesis of magnetically carbon nanotube modified with biological polymeric	383:466	In this study, a novel synthesis of magnetically carbon nanotube modified with biological polymeric was successfully prepared.
31715227	0	19	theme	Adsorptive	0:9	arg1	removal					11:17	Adsorptive removal	0:17	Adsorptive removal of endocrine disrupting compounds from aqueous solutions using magnetic multi-wall carbon	0:107	Adsorptive removal of endocrine disrupting compounds from aqueous solutions using magnetic multi-wall carbon nanotubes modified with chitosan biopolymer based on response surface methodology: Functionalization, kinetics, and isotherms studies.
31715227	6	20	theme	carbon	822:827	arg1	size					839:842	increased carbon nano tube size	812:842	increased carbon nano tube size	812:842	The surface morphology of nanocomposite was observed, increased carbon nano tube size, and the levels after surface deposition were completely covered by chitosan proteins.
31715227	9	21	theme	monolayer	1212:1220	arg1	capacity					1233:1240	The maximum monolayer adsorption capacity	1200:1240	The maximum monolayer adsorption capacity of nanocomposite for BPA	1200:1265	The maximum monolayer adsorption capacity of nanocomposite for BPA was 46.2 mg/g at 20 °C.
31715227	8	22	theme	Langmuir	1141:1148	arg1	model					1159:1163	the Langmuir isotherm model	1137:1163	the Langmuir isotherm model	1137:1163	The data obtained in this study followed the Langmuir isotherm model and the pseudo-second order model.
31715227	0	23	theme	endocrine	22:30	arg1	compounds					43:51	endocrine disrupting compounds	22:51	endocrine disrupting compounds	22:51	Adsorptive removal of endocrine disrupting compounds from aqueous solutions using magnetic multi-wall carbon nanotubes modified with chitosan biopolymer based on response surface methodology: Functionalization, kinetics, and isotherms studies.
31715227	7	24	theme	dosage	1070:1075	arg1	1 g/L					1077:1081	adsorbent dosage 1 g/L	1060:1081	adsorbent dosage 1 g/L	1060:1081	The results of our experiments showed that optimum adsorption conditions was achieved at t = 76 min, BPA concentration 6.5 mg/L, adsorbent dosage 1 g/L and pH = 6.2.
31715227	6	25	theme	surface	762:768	arg1	morphology					770:779	The surface morphology	758:779	The surface morphology of nanocomposite	758:796	The surface morphology of nanocomposite was observed, increased carbon nano tube size, and the levels after surface deposition were completely covered by chitosan proteins.
31715227	7	26	theme	experiments	950:960	arg1	results					935:941	The results	931:941	The results of our experiments	931:960	The results of our experiments showed that optimum adsorption conditions was achieved at t = 76 min, BPA concentration 6.5 mg/L, adsorbent dosage 1 g/L and pH = 6.2.
31715227	0	27	theme	chitosan	133:140	arg1	biopolymer					142:151	chitosan biopolymer	133:151	chitosan biopolymer based on response surface methodology	133:189	Adsorptive removal of endocrine disrupting compounds from aqueous solutions using magnetic multi-wall carbon nanotubes modified with chitosan biopolymer based on response surface methodology: Functionalization, kinetics, and isotherms studies.
31715227	2	28	theme	nanotube	424:431	arg1	synthesis					391:399	a novel synthesis	383:399	a novel synthesis of magnetically carbon nanotube modified with biological polymeric	383:466	In this study, a novel synthesis of magnetically carbon nanotube modified with biological polymeric was successfully prepared.
31715227	0	29	theme	compounds	43:51	arg1	removal					11:17	Adsorptive removal	0:17	Adsorptive removal of endocrine disrupting compounds from aqueous solutions using magnetic multi-wall carbon	0:107	Adsorptive removal of endocrine disrupting compounds from aqueous solutions using magnetic multi-wall carbon nanotubes modified with chitosan biopolymer based on response surface methodology: Functionalization, kinetics, and isotherms studies.
31715227	4	30	theme	Composite	651:659	arg1	Design					661:666	the Central Composite Design	639:666	the Central Composite Design	639:666	A prediction model for BPA adsorption was extended based on the Central Composite Design.
31715227	2	31	theme	carbon	417:422	arg1	nanotube					424:431	magnetically carbon nanotube	404:431	magnetically carbon nanotube modified with biological polymeric	404:466	In this study, a novel synthesis of magnetically carbon nanotube modified with biological polymeric was successfully prepared.
31715227	0	32	theme	disrupting	32:41	arg1	compounds					43:51	endocrine disrupting compounds	22:51	endocrine disrupting compounds	22:51	Adsorptive removal of endocrine disrupting compounds from aqueous solutions using magnetic multi-wall carbon nanotubes modified with chitosan biopolymer based on response surface methodology: Functionalization, kinetics, and isotherms studies.
31715227	4	33	theme	prediction	581:590	arg1	model					592:596	A prediction model	579:596	A prediction model for BPA adsorption	579:615	A prediction model for BPA adsorption was extended based on the Central Composite Design.
31715227	9	34	theme	nanocomposite	1245:1257	arg1	capacity					1233:1240	The maximum monolayer adsorption capacity	1200:1240	The maximum monolayer adsorption capacity of nanocomposite for BPA	1200:1265	The maximum monolayer adsorption capacity of nanocomposite for BPA was 46.2 mg/g at 20 °C.
31715227	0	35	dep	Functionalization	192:208	arg1	studies					235:241	studies	235:241	studies	235:241	Adsorptive removal of endocrine disrupting compounds from aqueous solutions using magnetic multi-wall carbon nanotubes modified with chitosan biopolymer based on response surface methodology: Functionalization, kinetics, and isotherms studies.
31715227	0	36	theme	aqueous	58:64	arg1	solutions					66:74	aqueous solutions	58:74	aqueous solutions using magnetic multi-wall carbon	58:107	Adsorptive removal of endocrine disrupting compounds from aqueous solutions using magnetic multi-wall carbon nanotubes modified with chitosan biopolymer based on response surface methodology: Functionalization, kinetics, and isotherms studies.
31715227	0	37	theme	surface	171:177	arg1	methodology					179:189	response surface methodology	162:189	response surface methodology	162:189	Adsorptive removal of endocrine disrupting compounds from aqueous solutions using magnetic multi-wall carbon nanotubes modified with chitosan biopolymer based on response surface methodology: Functionalization, kinetics, and isotherms studies.
31715227	0	38	from	solutions	66:74	arg1	removal					11:17	Adsorptive removal	0:17	Adsorptive removal of endocrine disrupting compounds from aqueous solutions using magnetic multi-wall carbon	0:107	Adsorptive removal of endocrine disrupting compounds from aqueous solutions using magnetic multi-wall carbon nanotubes modified with chitosan biopolymer based on response surface methodology: Functionalization, kinetics, and isotherms studies.
31715227	1	39	attach	presence	258:265	arg2	compounds					291:299	endocrine disrupting compounds	270:299	endocrine disrupting compounds	270:299	Recently, the presence of endocrine disrupting compounds in the environment has emerged as a global and ubiquitous problem.
31715227	1	39	attach	presence	258:265	arg1	environment					308:318	the environment	304:318	the environment	304:318	Recently, the presence of endocrine disrupting compounds in the environment has emerged as a global and ubiquitous problem.
31715227	9	40	theme	adsorption	1222:1231	arg1	capacity					1233:1240	The maximum monolayer adsorption capacity	1200:1240	The maximum monolayer adsorption capacity of nanocomposite for BPA	1200:1265	The maximum monolayer adsorption capacity of nanocomposite for BPA was 46.2 mg/g at 20 °C.
31715227	3	41	theme	different	509:517	arg1	parameters					519:528	different parameters	509:528	different parameters	509:528	The effect of different parameters on the Bisphenol A (BPA) adsorption was studied.
31715227	7	42	theme	adsorbent	1060:1068	arg1	1 g/L					1077:1081	adsorbent dosage 1 g/L	1060:1081	adsorbent dosage 1 g/L	1060:1081	The results of our experiments showed that optimum adsorption conditions was achieved at t = 76 min, BPA concentration 6.5 mg/L, adsorbent dosage 1 g/L and pH = 6.2.
31715227	0	43	theme	response	162:169	arg1	methodology					179:189	response surface methodology	162:189	response surface methodology	162:189	Adsorptive removal of endocrine disrupting compounds from aqueous solutions using magnetic multi-wall carbon nanotubes modified with chitosan biopolymer based on response surface methodology: Functionalization, kinetics, and isotherms studies.
31715227	10	44	theme	BPA	1332:1334	arg1	spontaneous					1359:1369	spontaneous	1359:1369	spontaneous	1359:1369	This study showed that the adsorption of BPA onto nanocomposite was spontaneous and thermodynamically desirable.
31715227	10	44	theme	BPA	1332:1334	arg1	adsorption					1318:1327	the adsorption	1314:1327	the adsorption of BPA onto nanocomposite	1314:1353	This study showed that the adsorption of BPA onto nanocomposite was spontaneous and thermodynamically desirable.
31715227	3	45	theme	parameters	519:528	arg1	effect					499:504	The effect	495:504	The effect of different parameters on the Bisphenol A (BPA) adsorption	495:564	The effect of different parameters on the Bisphenol A (BPA) adsorption was studied.
31715227	1	46	theme	global	337:342	arg1	problem					359:365	a global and ubiquitous problem	335:365	a global and ubiquitous problem	335:365	Recently, the presence of endocrine disrupting compounds in the environment has emerged as a global and ubiquitous problem.
31715227	5	47	theme	biopolymeric	688:699	arg1	nanotubes					701:709	the prepared biopolymeric nanotubes	675:709	the prepared biopolymeric nanotubes	675:709	Also, the prepared biopolymeric nanotubes were characterized by FT-IR, XRD, TEM, FE-SEM.
31715227	6	48	theme	tube	834:837	arg1	size					839:842	increased carbon nano tube size	812:842	increased carbon nano tube size	812:842	The surface morphology of nanocomposite was observed, increased carbon nano tube size, and the levels after surface deposition were completely covered by chitosan proteins.
31715227	7	49	theme	BPA	1032:1034	arg1	6.5 mg/L					1050:1057	BPA concentration 6.5 mg/L	1032:1057	BPA concentration 6.5 mg/L	1032:1057	The results of our experiments showed that optimum adsorption conditions was achieved at t = 76 min, BPA concentration 6.5 mg/L, adsorbent dosage 1 g/L and pH = 6.2.
31715227	6	50	theme	nano	829:832	arg1	size					839:842	increased carbon nano tube size	812:842	increased carbon nano tube size	812:842	The surface morphology of nanocomposite was observed, increased carbon nano tube size, and the levels after surface deposition were completely covered by chitosan proteins.
31715227	7	51	theme	adsorption	982:991	arg1	conditions					993:1002	optimum adsorption conditions	974:1002	optimum adsorption conditions	974:1002	The results of our experiments showed that optimum adsorption conditions was achieved at t = 76 min, BPA concentration 6.5 mg/L, adsorbent dosage 1 g/L and pH = 6.2.
31715227	4	52	theme	Central	643:649	arg1	Design					661:666	the Central Composite Design	639:666	the Central Composite Design	639:666	A prediction model for BPA adsorption was extended based on the Central Composite Design.
31715227	3	53	theme	Bisphenol	537:545	arg1	BPA					550:552	BPA	550:552	BPA	550:552	The effect of different parameters on the Bisphenol A (BPA) adsorption was studied.
31715227	3	53	theme	Bisphenol	537:545	arg1	A					547:547	Bisphenol A	537:547	the Bisphenol A (BPA) adsorption	533:564	The effect of different parameters on the Bisphenol A (BPA) adsorption was studied.
31715227	4	54	theme	BPA	602:604	arg1	adsorption					606:615	BPA adsorption	602:615	BPA adsorption	602:615	A prediction model for BPA adsorption was extended based on the Central Composite Design.
31715227	6	55	theme	chitosan	912:919	arg1	proteins					921:928	chitosan proteins	912:928	chitosan proteins	912:928	The surface morphology of nanocomposite was observed, increased carbon nano tube size, and the levels after surface deposition were completely covered by chitosan proteins.
31715227	3	56	theme	A	547:547	arg1	adsorption					555:564	the Bisphenol A (BPA) adsorption	533:564	the Bisphenol A (BPA) adsorption	533:564	The effect of different parameters on the Bisphenol A (BPA) adsorption was studied.
31715227	5	57	theme	prepared	679:686	arg1	nanotubes					701:709	the prepared biopolymeric nanotubes	675:709	the prepared biopolymeric nanotubes	675:709	Also, the prepared biopolymeric nanotubes were characterized by FT-IR, XRD, TEM, FE-SEM.
31320068	5	0	theme	DLD1	647:650	arg1	xenograft					652:660	DLD1 xenograft and AOM-DSS induced tumor models	647:693	xenograft	652:660	The data shows that BG136 alone decreases the tumor burdens in DLD1 xenograft and AOM-DSS induced tumor models.
31320068	6	1	theme	antitumor	720:728	arg1	effects					730:736	the antitumor effects	716:736	the antitumor effects of PD-1 antibody in B16 syngeneic tumor model	716:782	BG136 also augments the antitumor effects of PD-1 antibody in B16 syngeneic tumor model.
31320068	1	2	theme	immune	164:169	arg1	cells					171:175	innate immune cells	157:175	innate immune cells	157:175	β-glucans trigger the proinflammatory responses of innate immune cells to enhance the host defense.
31320068	5	3	theme	induced	674:680	arg1	models					688:693	DLD1 xenograft and AOM-DSS induced tumor models	647:693	models	688:693	The data shows that BG136 alone decreases the tumor burdens in DLD1 xenograft and AOM-DSS induced tumor models.
31320068	1	4	theme	cells	171:175	arg1	responses					144:152	the proinflammatory responses	124:152	the proinflammatory responses of innate immune cells	124:175	β-glucans trigger the proinflammatory responses of innate immune cells to enhance the host defense.
31320068	7	5	theme	intratumoral	895:906	arg1	composition					920:930	the systemic and intratumoral immune cell composition	878:930	the systemic and intratumoral immune cell composition	878:930	BG136 increases macrophage phagocytosis, enhances cytokine/chemokine secretion and modulates the systemic and intratumoral immune cell composition.
31320068	8	6	theme	immune	993:998	arg1	BG136					971:975	BG136	971:975	BG136	971:975	Collectively, these data suggest that BG136 might act as an immune stimulator to exert antitumor effects in vivo.
31320068	8	6	theme	immune	993:998	arg1	stimulator					1000:1009	an immune stimulator	990:1009	an immune stimulator	990:1009	Collectively, these data suggest that BG136 might act as an immune stimulator to exert antitumor effects in vivo.
31320068	7	7	theme	immune	908:913	arg1	composition					920:930	the systemic and intratumoral immune cell composition	878:930	the systemic and intratumoral immune cell composition	878:930	BG136 increases macrophage phagocytosis, enhances cytokine/chemokine secretion and modulates the systemic and intratumoral immune cell composition.
31320068	0	8	theme	immune	87:92	arg1	stimulator					94:103	an immune stimulator	84:103	an immune stimulator	84:103	A β-1,3/1,6-glucan from Durvillaea Antarctica inhibits tumor progression in vivo as an immune stimulator.
31320068	0	8	theme	immune	87:92	arg1	progression					61:71	tumor progression	55:71	tumor progression	55:71	A β-1,3/1,6-glucan from Durvillaea Antarctica inhibits tumor progression in vivo as an immune stimulator.
31320068	4	9	theme	present	494:500	arg1	study					502:506	the present study	490:506	the present study	490:506	In the present study, we further explored its antitumor effects in vivo as an immune stimulator.
31320068	5	10	theme	AOM-DSS	666:672	arg1	models					688:693	DLD1 xenograft and AOM-DSS induced tumor models	647:693	models	688:693	The data shows that BG136 alone decreases the tumor burdens in DLD1 xenograft and AOM-DSS induced tumor models.
31320068	2	11	theme	antitumor	285:293	arg1	activities					295:304	exerted antitumor activities	277:304	exerted antitumor activities	277:304	A variety of β-glucans were identified as strong immune stimulator and exerted antitumor activities.
31320068	2	11	theme	antitumor	285:293	arg1	β-glucans					219:227	β-glucans	219:227	β-glucans	219:227	A variety of β-glucans were identified as strong immune stimulator and exerted antitumor activities.
31320068	2	11	theme	antitumor	285:293	arg1	variety					208:214	A variety	206:214	A variety of β-glucans	206:227	A variety of β-glucans were identified as strong immune stimulator and exerted antitumor activities.
31320068	7	12	theme	macrophage	801:810	arg1	phagocytosis					812:823	macrophage phagocytosis	801:823	macrophage phagocytosis	801:823	BG136 increases macrophage phagocytosis, enhances cytokine/chemokine secretion and modulates the systemic and intratumoral immune cell composition.
31320068	7	13	theme	systemic	882:889	arg1	composition					920:930	the systemic and intratumoral immune cell composition	878:930	the systemic and intratumoral immune cell composition	878:930	BG136 increases macrophage phagocytosis, enhances cytokine/chemokine secretion and modulates the systemic and intratumoral immune cell composition.
31320068	2	14	theme	exerted	277:283	arg1	activities					295:304	exerted antitumor activities	277:304	exerted antitumor activities	277:304	A variety of β-glucans were identified as strong immune stimulator and exerted antitumor activities.
31320068	2	14	theme	exerted	277:283	arg1	β-glucans					219:227	β-glucans	219:227	β-glucans	219:227	A variety of β-glucans were identified as strong immune stimulator and exerted antitumor activities.
31320068	2	14	theme	exerted	277:283	arg1	variety					208:214	A variety	206:214	A variety of β-glucans	206:227	A variety of β-glucans were identified as strong immune stimulator and exerted antitumor activities.
31320068	3	15	theme	macrophage	456:465	arg1	line					472:475	macrophage cell line	456:475	macrophage cell line RAW264.7	456:484	Our previous work indicates that a β-1,3/1,6-glucan (BG136) derived from marina alga Durvillaea antarctica promotes the proinflammatory responses in macrophage cell line RAW264.7.
31320068	5	16	theme	tumor	630:634	arg1	burdens					636:642	the tumor burdens	626:642	the tumor burdens in DLD1 xenograft and AOM-DSS induced tumor models	626:693	The data shows that BG136 alone decreases the tumor burdens in DLD1 xenograft and AOM-DSS induced tumor models.
31320068	3	17	theme	cell	467:470	arg1	line					472:475	macrophage cell line	456:475	macrophage cell line RAW264.7	456:484	Our previous work indicates that a β-1,3/1,6-glucan (BG136) derived from marina alga Durvillaea antarctica promotes the proinflammatory responses in macrophage cell line RAW264.7.
31320068	3	18	theme	alga	387:390	arg1	antarctica					403:412	marina alga Durvillaea antarctica	380:412	marina alga Durvillaea antarctica	380:412	Our previous work indicates that a β-1,3/1,6-glucan (BG136) derived from marina alga Durvillaea antarctica promotes the proinflammatory responses in macrophage cell line RAW264.7.
31320068	0	19	from	Antarctica	35:44	arg1	β-1,3/1,6-glucan					2:17	A β-1,3/1,6-glucan	0:17	A β-1,3/1,6-glucan from Durvillaea Antarctica	0:44	A β-1,3/1,6-glucan from Durvillaea Antarctica inhibits tumor progression in vivo as an immune stimulator.
31320068	8	20	theme	antitumor	1020:1028	arg1	effects					1030:1036	antitumor effects	1020:1036	antitumor effects	1020:1036	Collectively, these data suggest that BG136 might act as an immune stimulator to exert antitumor effects in vivo.
31320068	3	21	theme	Durvillaea	392:401	arg1	antarctica					403:412	marina alga Durvillaea antarctica	380:412	marina alga Durvillaea antarctica	380:412	Our previous work indicates that a β-1,3/1,6-glucan (BG136) derived from marina alga Durvillaea antarctica promotes the proinflammatory responses in macrophage cell line RAW264.7.
31320068	5	22	from	burdens	636:642	arg1	models					688:693	DLD1 xenograft and AOM-DSS induced tumor models	647:693	models	688:693	The data shows that BG136 alone decreases the tumor burdens in DLD1 xenograft and AOM-DSS induced tumor models.
31320068	5	22	from	burdens	636:642	arg1	xenograft					652:660	DLD1 xenograft and AOM-DSS induced tumor models	647:693	xenograft	652:660	The data shows that BG136 alone decreases the tumor burdens in DLD1 xenograft and AOM-DSS induced tumor models.
31320068	1	23	theme	host	192:195	arg1	defense					197:203	the host defense	188:203	the host defense	188:203	β-glucans trigger the proinflammatory responses of innate immune cells to enhance the host defense.
31320068	6	24	theme	PD-1	741:744	arg1	antibody					746:753	PD-1 antibody	741:753	PD-1 antibody	741:753	BG136 also augments the antitumor effects of PD-1 antibody in B16 syngeneic tumor model.
31320068	6	25	theme	tumor	772:776	arg1	model					778:782	B16 syngeneic tumor model	758:782	B16 syngeneic tumor model	758:782	BG136 also augments the antitumor effects of PD-1 antibody in B16 syngeneic tumor model.
31320068	3	26	theme	marina	380:385	arg1	antarctica					403:412	marina alga Durvillaea antarctica	380:412	marina alga Durvillaea antarctica	380:412	Our previous work indicates that a β-1,3/1,6-glucan (BG136) derived from marina alga Durvillaea antarctica promotes the proinflammatory responses in macrophage cell line RAW264.7.
31320068	4	27	theme	immune	565:570	arg1	effects					543:549	its antitumor effects	529:549	its antitumor effects	529:549	In the present study, we further explored its antitumor effects in vivo as an immune stimulator.
31320068	4	27	theme	immune	565:570	arg1	stimulator					572:581	an immune stimulator	562:581	an immune stimulator	562:581	In the present study, we further explored its antitumor effects in vivo as an immune stimulator.
31320068	7	28	theme	cytokine/chemokine	835:852	arg1	secretion					854:862	cytokine/chemokine secretion	835:862	cytokine/chemokine secretion	835:862	BG136 increases macrophage phagocytosis, enhances cytokine/chemokine secretion and modulates the systemic and intratumoral immune cell composition.
31320068	3	29	theme	proinflammatory	427:441	arg1	responses					443:451	the proinflammatory responses	423:451	the proinflammatory responses in macrophage cell line RAW264.7	423:484	Our previous work indicates that a β-1,3/1,6-glucan (BG136) derived from marina alga Durvillaea antarctica promotes the proinflammatory responses in macrophage cell line RAW264.7.
31320068	6	30	theme	syngeneic	762:770	arg1	model					778:782	B16 syngeneic tumor model	758:782	B16 syngeneic tumor model	758:782	BG136 also augments the antitumor effects of PD-1 antibody in B16 syngeneic tumor model.
31320068	4	31	theme	antitumor	533:541	arg1	effects					543:549	its antitumor effects	529:549	its antitumor effects	529:549	In the present study, we further explored its antitumor effects in vivo as an immune stimulator.
31320068	4	31	theme	antitumor	533:541	arg1	stimulator					572:581	an immune stimulator	562:581	an immune stimulator	562:581	In the present study, we further explored its antitumor effects in vivo as an immune stimulator.
31320068	3	32	theme	previous	311:318	arg1	work					320:323	Our previous work	307:323	Our previous work	307:323	Our previous work indicates that a β-1,3/1,6-glucan (BG136) derived from marina alga Durvillaea antarctica promotes the proinflammatory responses in macrophage cell line RAW264.7.
31320068	2	33	theme	β-glucans	219:227	arg1	activities					295:304	exerted antitumor activities	277:304	exerted antitumor activities	277:304	A variety of β-glucans were identified as strong immune stimulator and exerted antitumor activities.
31320068	2	33	theme	β-glucans	219:227	arg1	stimulator					262:271	strong immune stimulator	248:271	strong immune stimulator	248:271	A variety of β-glucans were identified as strong immune stimulator and exerted antitumor activities.
31320068	2	33	theme	β-glucans	219:227	arg1	β-glucans					219:227	β-glucans	219:227	β-glucans	219:227	A variety of β-glucans were identified as strong immune stimulator and exerted antitumor activities.
31320068	2	33	theme	β-glucans	219:227	arg1	variety					208:214	A variety	206:214	A variety of β-glucans	206:227	A variety of β-glucans were identified as strong immune stimulator and exerted antitumor activities.
31320068	6	34	theme	B16	758:760	arg1	model					778:782	B16 syngeneic tumor model	758:782	B16 syngeneic tumor model	758:782	BG136 also augments the antitumor effects of PD-1 antibody in B16 syngeneic tumor model.
31320068	0	35	theme	tumor	55:59	arg1	stimulator					94:103	an immune stimulator	84:103	an immune stimulator	84:103	A β-1,3/1,6-glucan from Durvillaea Antarctica inhibits tumor progression in vivo as an immune stimulator.
31320068	0	35	theme	tumor	55:59	arg1	progression					61:71	tumor progression	55:71	tumor progression	55:71	A β-1,3/1,6-glucan from Durvillaea Antarctica inhibits tumor progression in vivo as an immune stimulator.
31320068	5	36	theme	tumor	682:686	arg1	models					688:693	DLD1 xenograft and AOM-DSS induced tumor models	647:693	models	688:693	The data shows that BG136 alone decreases the tumor burdens in DLD1 xenograft and AOM-DSS induced tumor models.
31320068	3	37	dep	line	472:475	arg1	RAW264.7					477:484	RAW264.7	477:484	macrophage cell line RAW264.7	456:484	Our previous work indicates that a β-1,3/1,6-glucan (BG136) derived from marina alga Durvillaea antarctica promotes the proinflammatory responses in macrophage cell line RAW264.7.
31320068	6	38	theme	antibody	746:753	arg1	effects					730:736	the antitumor effects	716:736	the antitumor effects of PD-1 antibody in B16 syngeneic tumor model	716:782	BG136 also augments the antitumor effects of PD-1 antibody in B16 syngeneic tumor model.
31320068	1	39	theme	proinflammatory	128:142	arg1	responses					144:152	the proinflammatory responses	124:152	the proinflammatory responses of innate immune cells	124:175	β-glucans trigger the proinflammatory responses of innate immune cells to enhance the host defense.
31320068	2	40	theme	immune	255:260	arg1	variety					208:214	A variety	206:214	A variety of β-glucans	206:227	A variety of β-glucans were identified as strong immune stimulator and exerted antitumor activities.
31320068	2	40	theme	immune	255:260	arg1	β-glucans					219:227	β-glucans	219:227	β-glucans	219:227	A variety of β-glucans were identified as strong immune stimulator and exerted antitumor activities.
31320068	2	40	theme	immune	255:260	arg1	stimulator					262:271	strong immune stimulator	248:271	strong immune stimulator	248:271	A variety of β-glucans were identified as strong immune stimulator and exerted antitumor activities.
31320068	3	41	from	responses	443:451	arg1	line					472:475	macrophage cell line	456:475	macrophage cell line RAW264.7	456:484	Our previous work indicates that a β-1,3/1,6-glucan (BG136) derived from marina alga Durvillaea antarctica promotes the proinflammatory responses in macrophage cell line RAW264.7.
31320068	2	42	theme	strong	248:253	arg1	variety					208:214	A variety	206:214	A variety of β-glucans	206:227	A variety of β-glucans were identified as strong immune stimulator and exerted antitumor activities.
31320068	2	42	theme	strong	248:253	arg1	β-glucans					219:227	β-glucans	219:227	β-glucans	219:227	A variety of β-glucans were identified as strong immune stimulator and exerted antitumor activities.
31320068	2	42	theme	strong	248:253	arg1	stimulator					262:271	strong immune stimulator	248:271	strong immune stimulator	248:271	A variety of β-glucans were identified as strong immune stimulator and exerted antitumor activities.
31320068	6	43	from	effects	730:736	arg1	model					778:782	B16 syngeneic tumor model	758:782	B16 syngeneic tumor model	758:782	BG136 also augments the antitumor effects of PD-1 antibody in B16 syngeneic tumor model.
31320068	3	44	attach	derived	367:373	arg2	BG136					360:364	BG136	360:364	BG136	360:364	Our previous work indicates that a β-1,3/1,6-glucan (BG136) derived from marina alga Durvillaea antarctica promotes the proinflammatory responses in macrophage cell line RAW264.7.
31320068	3	44	attach	derived	367:373	arg2	β-1,3/1,6-glucan					342:357	a β-1,3/1,6-glucan	340:357	a β-1,3/1,6-glucan (BG136) derived from marina alga Durvillaea antarctica	340:412	Our previous work indicates that a β-1,3/1,6-glucan (BG136) derived from marina alga Durvillaea antarctica promotes the proinflammatory responses in macrophage cell line RAW264.7.
31320068	3	44	attach	derived	367:373	arg1	antarctica					403:412	marina alga Durvillaea antarctica	380:412	marina alga Durvillaea antarctica	380:412	Our previous work indicates that a β-1,3/1,6-glucan (BG136) derived from marina alga Durvillaea antarctica promotes the proinflammatory responses in macrophage cell line RAW264.7.
31320068	1	45	theme	innate	157:162	arg1	cells					171:175	innate immune cells	157:175	innate immune cells	157:175	β-glucans trigger the proinflammatory responses of innate immune cells to enhance the host defense.
31320068	7	46	theme	cell	915:918	arg1	composition					920:930	the systemic and intratumoral immune cell composition	878:930	the systemic and intratumoral immune cell composition	878:930	BG136 increases macrophage phagocytosis, enhances cytokine/chemokine secretion and modulates the systemic and intratumoral immune cell composition.
31405262	2	0	theme	combination	384:394	arg1	effects					235:241	the effects	231:241	the effects of 2'-fucosyllactose (2'-FL), a mixture of short-chain galactooligosaccharides/long-chain fructooligosaccharides (scGOS/lcFOS) 9:1 and their combination (scGOS/lcFOS/2'-FL) on dysbiosis induced during rotavirus (RV) diarrhea in neonatal rats	231:483	The present study aimed to investigate the effects of 2'-fucosyllactose (2'-FL), a mixture of short-chain galactooligosaccharides/long-chain fructooligosaccharides (scGOS/lcFOS) 9:1 and their combination (scGOS/lcFOS/2'-FL) on dysbiosis induced during rotavirus (RV) diarrhea in neonatal rats, elucidating crosstalk between bacteria and the immune system.
31405262	4	1	from	expression	784:793	arg1	intestine					838:846	the small intestine	828:846	the small intestine	828:846	On day 5, RV SA11 was intragastrically delivered to induce infection and diarrhea assessment, microbiota composition, and gene expression of Toll-like receptors (TLRs) in the small intestine were studied.
31405262	1	2	theme	multiple	160:167	arg1	factors					183:189	multiple environmental factors	160:189	multiple environmental factors	160:189	Colonization of the gut in early life can be altered through multiple environmental factors.
31405262	6	3	theme	TLR5	1013:1016	arg1	expressions					1027:1037	TLR5 and TLR7 expressions	1013:1037	TLR5 and TLR7 expressions	1013:1037	RV infection increased TLR2 expression, whereas 2'-FL boosted TLR5 and TLR7 expressions and scGOS/lcFOS increased that of TLR9.
31405262	2	4	theme	short-chain	286:296	arg1	9:1					370:372	short-chain galactooligosaccharides/long-chain fructooligosaccharides (scGOS/lcFOS) 9:1	286:372	short-chain galactooligosaccharides/long-chain fructooligosaccharides (scGOS/lcFOS) 9:1	286:372	The present study aimed to investigate the effects of 2'-fucosyllactose (2'-FL), a mixture of short-chain galactooligosaccharides/long-chain fructooligosaccharides (scGOS/lcFOS) 9:1 and their combination (scGOS/lcFOS/2'-FL) on dysbiosis induced during rotavirus (RV) diarrhea in neonatal rats, elucidating crosstalk between bacteria and the immune system.
31405262	7	5	theme	groups	1276:1281	arg1	microbiota					1246:1255	microbiota	1246:1255	microbiota of the noninfected groups	1246:1281	RV-infected rats displayed an intestinal dysbiosis that was effectively prevented by the dietary interventions, and consequently, their microbiota was more similar to microbiota of the noninfected groups.
31405262	2	6	theme	mixture	275:281	arg1	effects					235:241	the effects	231:241	the effects of 2'-fucosyllactose (2'-FL), a mixture of short-chain galactooligosaccharides/long-chain fructooligosaccharides (scGOS/lcFOS) 9:1 and their combination (scGOS/lcFOS/2'-FL) on dysbiosis induced during rotavirus (RV) diarrhea in neonatal rats	231:483	The present study aimed to investigate the effects of 2'-fucosyllactose (2'-FL), a mixture of short-chain galactooligosaccharides/long-chain fructooligosaccharides (scGOS/lcFOS) 9:1 and their combination (scGOS/lcFOS/2'-FL) on dysbiosis induced during rotavirus (RV) diarrhea in neonatal rats, elucidating crosstalk between bacteria and the immune system.
31405262	5	7	theme	diarrhea	941:948	arg1	variables					917:925	clinical variables	908:925	clinical variables of RV-induced diarrhea	908:948	All dietary interventions showed reduction in clinical variables of RV-induced diarrhea.
31405262	5	8	from	reduction	895:903	arg1	variables					917:925	clinical variables	908:925	clinical variables of RV-induced diarrhea	908:948	All dietary interventions showed reduction in clinical variables of RV-induced diarrhea.
31405262	3	9	theme	dietary	552:558	arg1	interventions					560:572	The dietary interventions	548:572	The dietary interventions	548:572	The dietary interventions were administered daily by oral gavage at days 2-8 of life in neonatal Lewis rats.
31405262	8	10	from	changes	1414:1420	arg1	crosstalk					1429:1437	the crosstalk	1425:1437	the crosstalk between gut microbiota and the innate immune system	1425:1489	The preventive effect of 2'-FL, scGOS/lcFOS, and their combination on dysbiosis associated to RV diarrhea in rats could be due to changes in the crosstalk between gut microbiota and the innate immune system.
31405262	1	11	theme	environmental	169:181	arg1	factors					183:189	multiple environmental factors	160:189	multiple environmental factors	160:189	Colonization of the gut in early life can be altered through multiple environmental factors.
31405262	2	12	theme	immune	533:538	arg1	system					540:545	the immune system	529:545	the immune system	529:545	The present study aimed to investigate the effects of 2'-fucosyllactose (2'-FL), a mixture of short-chain galactooligosaccharides/long-chain fructooligosaccharides (scGOS/lcFOS) 9:1 and their combination (scGOS/lcFOS/2'-FL) on dysbiosis induced during rotavirus (RV) diarrhea in neonatal rats, elucidating crosstalk between bacteria and the immune system.
31405262	5	13	theme	clinical	908:915	arg1	variables					917:925	clinical variables	908:925	clinical variables of RV-induced diarrhea	908:948	All dietary interventions showed reduction in clinical variables of RV-induced diarrhea.
31405262	4	14	theme	small	832:836	arg1	intestine					838:846	the small intestine	828:846	the small intestine	828:846	On day 5, RV SA11 was intragastrically delivered to induce infection and diarrhea assessment, microbiota composition, and gene expression of Toll-like receptors (TLRs) in the small intestine were studied.
31405262	4	15	from	composition	762:772	arg1	intestine					838:846	the small intestine	828:846	the small intestine	828:846	On day 5, RV SA11 was intragastrically delivered to induce infection and diarrhea assessment, microbiota composition, and gene expression of Toll-like receptors (TLRs) in the small intestine were studied.
31405262	4	16	theme	gene	779:782	arg1	expression					784:793	gene expression	779:793	gene expression	779:793	On day 5, RV SA11 was intragastrically delivered to induce infection and diarrhea assessment, microbiota composition, and gene expression of Toll-like receptors (TLRs) in the small intestine were studied.
31405262	3	17	dep	days	616:619	arg1	2-8					621:623	2-8	621:623	2-8	621:623	The dietary interventions were administered daily by oral gavage at days 2-8 of life in neonatal Lewis rats.
31405262	3	17	dep	days	616:619	arg1	life					628:631	life	628:631	life	628:631	The dietary interventions were administered daily by oral gavage at days 2-8 of life in neonatal Lewis rats.
31405262	2	18	theme	neonatal	471:478	arg1	rats					480:483	neonatal rats	471:483	neonatal rats	471:483	The present study aimed to investigate the effects of 2'-fucosyllactose (2'-FL), a mixture of short-chain galactooligosaccharides/long-chain fructooligosaccharides (scGOS/lcFOS) 9:1 and their combination (scGOS/lcFOS/2'-FL) on dysbiosis induced during rotavirus (RV) diarrhea in neonatal rats, elucidating crosstalk between bacteria and the immune system.
31405262	6	19	theme	TLR2	974:977	arg1	expression					979:988	TLR2 expression	974:988	TLR2 expression	974:988	RV infection increased TLR2 expression, whereas 2'-FL boosted TLR5 and TLR7 expressions and scGOS/lcFOS increased that of TLR9.
31405262	3	20	theme	oral	601:604	arg1	gavage					606:611	oral gavage	601:611	oral gavage	601:611	The dietary interventions were administered daily by oral gavage at days 2-8 of life in neonatal Lewis rats.
31405262	2	21	from	effects	235:241	arg1	dysbiosis					419:427	dysbiosis	419:427	dysbiosis induced during rotavirus (RV) diarrhea in neonatal rats	419:483	The present study aimed to investigate the effects of 2'-fucosyllactose (2'-FL), a mixture of short-chain galactooligosaccharides/long-chain fructooligosaccharides (scGOS/lcFOS) 9:1 and their combination (scGOS/lcFOS/2'-FL) on dysbiosis induced during rotavirus (RV) diarrhea in neonatal rats, elucidating crosstalk between bacteria and the immune system.
31405262	5	22	theme	dietary	866:872	arg1	interventions					874:886	All dietary interventions	862:886	All dietary interventions	862:886	All dietary interventions showed reduction in clinical variables of RV-induced diarrhea.
31405262	7	23	theme	intestinal	1109:1118	arg1	dysbiosis					1120:1128	an intestinal dysbiosis	1106:1128	an intestinal dysbiosis that was effectively prevented by the dietary interventions	1106:1188	RV-infected rats displayed an intestinal dysbiosis that was effectively prevented by the dietary interventions, and consequently, their microbiota was more similar to microbiota of the noninfected groups.
31405262	2	24	theme	fructooligosaccharides	333:354	arg1	9:1					370:372	short-chain galactooligosaccharides/long-chain fructooligosaccharides (scGOS/lcFOS) 9:1	286:372	short-chain galactooligosaccharides/long-chain fructooligosaccharides (scGOS/lcFOS) 9:1	286:372	The present study aimed to investigate the effects of 2'-fucosyllactose (2'-FL), a mixture of short-chain galactooligosaccharides/long-chain fructooligosaccharides (scGOS/lcFOS) 9:1 and their combination (scGOS/lcFOS/2'-FL) on dysbiosis induced during rotavirus (RV) diarrhea in neonatal rats, elucidating crosstalk between bacteria and the immune system.
31405262	4	25	theme	RV	667:668	arg1	SA11					670:673	RV SA11	667:673	RV SA11	667:673	On day 5, RV SA11 was intragastrically delivered to induce infection and diarrhea assessment, microbiota composition, and gene expression of Toll-like receptors (TLRs) in the small intestine were studied.
31405262	8	26	theme	gut	1447:1449	arg1	microbiota					1451:1460	gut microbiota	1447:1460	gut microbiota	1447:1460	The preventive effect of 2'-FL, scGOS/lcFOS, and their combination on dysbiosis associated to RV diarrhea in rats could be due to changes in the crosstalk between gut microbiota and the innate immune system.
31405262	4	27	from	assessment	739:748	arg1	intestine					838:846	the small intestine	828:846	the small intestine	828:846	On day 5, RV SA11 was intragastrically delivered to induce infection and diarrhea assessment, microbiota composition, and gene expression of Toll-like receptors (TLRs) in the small intestine were studied.
31405262	2	28	theme	galactooligosaccharides/long-chain	298:331	arg1	9:1					370:372	short-chain galactooligosaccharides/long-chain fructooligosaccharides (scGOS/lcFOS) 9:1	286:372	short-chain galactooligosaccharides/long-chain fructooligosaccharides (scGOS/lcFOS) 9:1	286:372	The present study aimed to investigate the effects of 2'-fucosyllactose (2'-FL), a mixture of short-chain galactooligosaccharides/long-chain fructooligosaccharides (scGOS/lcFOS) 9:1 and their combination (scGOS/lcFOS/2'-FL) on dysbiosis induced during rotavirus (RV) diarrhea in neonatal rats, elucidating crosstalk between bacteria and the immune system.
31405262	3	29	from	days	616:619	arg1	rats					651:654	neonatal Lewis rats	636:654	neonatal Lewis rats	636:654	The dietary interventions were administered daily by oral gavage at days 2-8 of life in neonatal Lewis rats.
31405262	2	30	theme	RV	455:456	arg1	diarrhea					459:466	rotavirus (RV) diarrhea	444:466	rotavirus (RV) diarrhea in neonatal rats	444:483	The present study aimed to investigate the effects of 2'-fucosyllactose (2'-FL), a mixture of short-chain galactooligosaccharides/long-chain fructooligosaccharides (scGOS/lcFOS) 9:1 and their combination (scGOS/lcFOS/2'-FL) on dysbiosis induced during rotavirus (RV) diarrhea in neonatal rats, elucidating crosstalk between bacteria and the immune system.
31405262	0	31	theme	Rotavirus-Associated	26:45	arg1	Dysbiosis					47:55	Rotavirus-Associated Dysbiosis	26:55	Rotavirus-Associated Dysbiosis	26:55	Oligosaccharides Modulate Rotavirus-Associated Dysbiosis and TLR Gene Expression in Neonatal Rats.
31405262	1	32	theme	gut	119:121	arg1	Colonization					99:110	Colonization	99:110	Colonization of the gut in early life	99:135	Colonization of the gut in early life can be altered through multiple environmental factors.
31405262	8	33	theme	preventive	1288:1297	arg1	effect					1299:1304	The preventive effect	1284:1304	The preventive effect of 2'-FL, scGOS/lcFOS, and their combination on dysbiosis associated to RV diarrhea in rats	1284:1396	The preventive effect of 2'-FL, scGOS/lcFOS, and their combination on dysbiosis associated to RV diarrhea in rats could be due to changes in the crosstalk between gut microbiota and the innate immune system.
31405262	8	33	theme	preventive	1288:1297	arg1	due					1407:1409	due	1407:1409	due	1407:1409	The preventive effect of 2'-FL, scGOS/lcFOS, and their combination on dysbiosis associated to RV diarrhea in rats could be due to changes in the crosstalk between gut microbiota and the innate immune system.
31405262	0	34	theme	TLR	61:63	arg1	Expression					70:79	TLR Gene Expression	61:79	TLR Gene Expression	61:79	Oligosaccharides Modulate Rotavirus-Associated Dysbiosis and TLR Gene Expression in Neonatal Rats.
31405262	2	35	theme	9:1	370:372	arg1	mixture					275:281	a mixture	273:281	a mixture of short-chain galactooligosaccharides/long-chain fructooligosaccharides (scGOS/lcFOS) 9:1	273:372	The present study aimed to investigate the effects of 2'-fucosyllactose (2'-FL), a mixture of short-chain galactooligosaccharides/long-chain fructooligosaccharides (scGOS/lcFOS) 9:1 and their combination (scGOS/lcFOS/2'-FL) on dysbiosis induced during rotavirus (RV) diarrhea in neonatal rats, elucidating crosstalk between bacteria and the immune system.
31405262	2	35	theme	9:1	370:372	arg1	combination					384:394	their combination	378:394	their combination (scGOS/lcFOS/2'-FL)	378:414	The present study aimed to investigate the effects of 2'-fucosyllactose (2'-FL), a mixture of short-chain galactooligosaccharides/long-chain fructooligosaccharides (scGOS/lcFOS) 9:1 and their combination (scGOS/lcFOS/2'-FL) on dysbiosis induced during rotavirus (RV) diarrhea in neonatal rats, elucidating crosstalk between bacteria and the immune system.
31405262	2	35	theme	9:1	370:372	arg1	2'-FL					265:269	2'-FL	265:269	2'-FL	265:269	The present study aimed to investigate the effects of 2'-fucosyllactose (2'-FL), a mixture of short-chain galactooligosaccharides/long-chain fructooligosaccharides (scGOS/lcFOS) 9:1 and their combination (scGOS/lcFOS/2'-FL) on dysbiosis induced during rotavirus (RV) diarrhea in neonatal rats, elucidating crosstalk between bacteria and the immune system.
31405262	2	35	theme	9:1	370:372	arg1	2'-fucosyllactose					246:262	2'-fucosyllactose	246:262	2'-fucosyllactose (2'-FL)	246:270	The present study aimed to investigate the effects of 2'-fucosyllactose (2'-FL), a mixture of short-chain galactooligosaccharides/long-chain fructooligosaccharides (scGOS/lcFOS) 9:1 and their combination (scGOS/lcFOS/2'-FL) on dysbiosis induced during rotavirus (RV) diarrhea in neonatal rats, elucidating crosstalk between bacteria and the immune system.
31405262	2	35	theme	9:1	370:372	arg1	scGOS/lcFOS/2'-FL					397:413	scGOS/lcFOS/2'-FL	397:413	scGOS/lcFOS/2'-FL	397:413	The present study aimed to investigate the effects of 2'-fucosyllactose (2'-FL), a mixture of short-chain galactooligosaccharides/long-chain fructooligosaccharides (scGOS/lcFOS) 9:1 and their combination (scGOS/lcFOS/2'-FL) on dysbiosis induced during rotavirus (RV) diarrhea in neonatal rats, elucidating crosstalk between bacteria and the immune system.
31405262	8	36	theme	2'-FL	1309:1313	arg1	effect					1299:1304	The preventive effect	1284:1304	The preventive effect of 2'-FL, scGOS/lcFOS, and their combination on dysbiosis associated to RV diarrhea in rats	1284:1396	The preventive effect of 2'-FL, scGOS/lcFOS, and their combination on dysbiosis associated to RV diarrhea in rats could be due to changes in the crosstalk between gut microbiota and the innate immune system.
31405262	8	36	theme	2'-FL	1309:1313	arg1	due					1407:1409	due	1407:1409	due	1407:1409	The preventive effect of 2'-FL, scGOS/lcFOS, and their combination on dysbiosis associated to RV diarrhea in rats could be due to changes in the crosstalk between gut microbiota and the innate immune system.
31405262	2	37	theme	rotavirus	444:452	arg1	diarrhea					459:466	rotavirus (RV) diarrhea	444:466	rotavirus (RV) diarrhea in neonatal rats	444:483	The present study aimed to investigate the effects of 2'-fucosyllactose (2'-FL), a mixture of short-chain galactooligosaccharides/long-chain fructooligosaccharides (scGOS/lcFOS) 9:1 and their combination (scGOS/lcFOS/2'-FL) on dysbiosis induced during rotavirus (RV) diarrhea in neonatal rats, elucidating crosstalk between bacteria and the immune system.
31405262	7	38	theme	RV-infected	1079:1089	arg1	rats					1091:1094	RV-infected rats	1079:1094	RV-infected rats	1079:1094	RV-infected rats displayed an intestinal dysbiosis that was effectively prevented by the dietary interventions, and consequently, their microbiota was more similar to microbiota of the noninfected groups.
31405262	8	39	theme	innate	1470:1475	arg1	system					1484:1489	the innate immune system	1466:1489	the innate immune system	1466:1489	The preventive effect of 2'-FL, scGOS/lcFOS, and their combination on dysbiosis associated to RV diarrhea in rats could be due to changes in the crosstalk between gut microbiota and the innate immune system.
31405262	8	40	theme	scGOS/lcFOS	1316:1326	arg1	effect					1299:1304	The preventive effect	1284:1304	The preventive effect of 2'-FL, scGOS/lcFOS, and their combination on dysbiosis associated to RV diarrhea in rats	1284:1396	The preventive effect of 2'-FL, scGOS/lcFOS, and their combination on dysbiosis associated to RV diarrhea in rats could be due to changes in the crosstalk between gut microbiota and the innate immune system.
31405262	8	40	theme	scGOS/lcFOS	1316:1326	arg1	due					1407:1409	due	1407:1409	due	1407:1409	The preventive effect of 2'-FL, scGOS/lcFOS, and their combination on dysbiosis associated to RV diarrhea in rats could be due to changes in the crosstalk between gut microbiota and the innate immune system.
31405262	0	41	theme	Gene	65:68	arg1	Expression					70:79	TLR Gene Expression	61:79	TLR Gene Expression	61:79	Oligosaccharides Modulate Rotavirus-Associated Dysbiosis and TLR Gene Expression in Neonatal Rats.
31405262	6	42	theme	RV	951:952	arg1	infection					954:962	RV infection	951:962	RV infection	951:962	RV infection increased TLR2 expression, whereas 2'-FL boosted TLR5 and TLR7 expressions and scGOS/lcFOS increased that of TLR9.
31405262	8	43	theme	immune	1477:1482	arg1	system					1484:1489	the innate immune system	1466:1489	the innate immune system	1466:1489	The preventive effect of 2'-FL, scGOS/lcFOS, and their combination on dysbiosis associated to RV diarrhea in rats could be due to changes in the crosstalk between gut microbiota and the innate immune system.
31405262	1	44	theme	early	126:130	arg1	life					132:135	early life	126:135	early life	126:135	Colonization of the gut in early life can be altered through multiple environmental factors.
31405262	8	45	from	diarrhea	1381:1388	arg1	rats					1393:1396	rats	1393:1396	rats	1393:1396	The preventive effect of 2'-FL, scGOS/lcFOS, and their combination on dysbiosis associated to RV diarrhea in rats could be due to changes in the crosstalk between gut microbiota and the innate immune system.
31405262	4	46	theme	receptors	808:816	arg1	assessment					739:748	diarrhea assessment	730:748	diarrhea assessment	730:748	On day 5, RV SA11 was intragastrically delivered to induce infection and diarrhea assessment, microbiota composition, and gene expression of Toll-like receptors (TLRs) in the small intestine were studied.
31405262	4	46	theme	receptors	808:816	arg1	composition					762:772	microbiota composition	751:772	microbiota composition	751:772	On day 5, RV SA11 was intragastrically delivered to induce infection and diarrhea assessment, microbiota composition, and gene expression of Toll-like receptors (TLRs) in the small intestine were studied.
31405262	4	46	theme	receptors	808:816	arg1	expression					784:793	gene expression	779:793	gene expression	779:793	On day 5, RV SA11 was intragastrically delivered to induce infection and diarrhea assessment, microbiota composition, and gene expression of Toll-like receptors (TLRs) in the small intestine were studied.
31405262	7	47	theme	dietary	1168:1174	arg1	interventions					1176:1188	the dietary interventions	1164:1188	the dietary interventions	1164:1188	RV-infected rats displayed an intestinal dysbiosis that was effectively prevented by the dietary interventions, and consequently, their microbiota was more similar to microbiota of the noninfected groups.
31405262	4	48	theme	Toll-like	798:806	arg1	receptors					808:816	Toll-like receptors	798:816	Toll-like receptors (TLRs)	798:823	On day 5, RV SA11 was intragastrically delivered to induce infection and diarrhea assessment, microbiota composition, and gene expression of Toll-like receptors (TLRs) in the small intestine were studied.
31405262	4	48	theme	Toll-like	798:806	arg1	TLRs					819:822	TLRs	819:822	TLRs	819:822	On day 5, RV SA11 was intragastrically delivered to induce infection and diarrhea assessment, microbiota composition, and gene expression of Toll-like receptors (TLRs) in the small intestine were studied.
31405262	3	49	theme	neonatal	636:643	arg1	rats					651:654	neonatal Lewis rats	636:654	neonatal Lewis rats	636:654	The dietary interventions were administered daily by oral gavage at days 2-8 of life in neonatal Lewis rats.
31405262	4	50	theme	diarrhea	730:737	arg1	assessment					739:748	diarrhea assessment	730:748	diarrhea assessment	730:748	On day 5, RV SA11 was intragastrically delivered to induce infection and diarrhea assessment, microbiota composition, and gene expression of Toll-like receptors (TLRs) in the small intestine were studied.
31405262	8	51	theme	RV	1378:1379	arg1	diarrhea					1381:1388	RV diarrhea	1378:1388	RV diarrhea in rats	1378:1396	The preventive effect of 2'-FL, scGOS/lcFOS, and their combination on dysbiosis associated to RV diarrhea in rats could be due to changes in the crosstalk between gut microbiota and the innate immune system.
31405262	3	52	theme	Lewis	645:649	arg1	rats					651:654	neonatal Lewis rats	636:654	neonatal Lewis rats	636:654	The dietary interventions were administered daily by oral gavage at days 2-8 of life in neonatal Lewis rats.
31405262	4	53	theme	microbiota	751:760	arg1	composition					762:772	microbiota composition	751:772	microbiota composition	751:772	On day 5, RV SA11 was intragastrically delivered to induce infection and diarrhea assessment, microbiota composition, and gene expression of Toll-like receptors (TLRs) in the small intestine were studied.
31405262	2	54	theme	scGOS/lcFOS	357:367	arg1	9:1					370:372	short-chain galactooligosaccharides/long-chain fructooligosaccharides (scGOS/lcFOS) 9:1	286:372	short-chain galactooligosaccharides/long-chain fructooligosaccharides (scGOS/lcFOS) 9:1	286:372	The present study aimed to investigate the effects of 2'-fucosyllactose (2'-FL), a mixture of short-chain galactooligosaccharides/long-chain fructooligosaccharides (scGOS/lcFOS) 9:1 and their combination (scGOS/lcFOS/2'-FL) on dysbiosis induced during rotavirus (RV) diarrhea in neonatal rats, elucidating crosstalk between bacteria and the immune system.
31405262	1	55	from	Colonization	99:110	arg1	life					132:135	early life	126:135	early life	126:135	Colonization of the gut in early life can be altered through multiple environmental factors.
31405262	5	56	theme	RV-induced	930:939	arg1	diarrhea					941:948	RV-induced diarrhea	930:948	RV-induced diarrhea	930:948	All dietary interventions showed reduction in clinical variables of RV-induced diarrhea.
31405262	0	57	theme	Neonatal	84:91	arg1	Rats					93:96	Neonatal Rats	84:96	Neonatal Rats	84:96	Oligosaccharides Modulate Rotavirus-Associated Dysbiosis and TLR Gene Expression in Neonatal Rats.
31405262	2	58	theme	present	196:202	arg1	study					204:208	The present study	192:208	The present study	192:208	The present study aimed to investigate the effects of 2'-fucosyllactose (2'-FL), a mixture of short-chain galactooligosaccharides/long-chain fructooligosaccharides (scGOS/lcFOS) 9:1 and their combination (scGOS/lcFOS/2'-FL) on dysbiosis induced during rotavirus (RV) diarrhea in neonatal rats, elucidating crosstalk between bacteria and the immune system.
31405262	3	59	theme	life	628:631	arg1	2-8					621:623	2-8	621:623	2-8	621:623	The dietary interventions were administered daily by oral gavage at days 2-8 of life in neonatal Lewis rats.
31405262	3	59	theme	life	628:631	arg1	life					628:631	life	628:631	life	628:631	The dietary interventions were administered daily by oral gavage at days 2-8 of life in neonatal Lewis rats.
31405262	8	60	theme	combination	1339:1349	arg1	effect					1299:1304	The preventive effect	1284:1304	The preventive effect of 2'-FL, scGOS/lcFOS, and their combination on dysbiosis associated to RV diarrhea in rats	1284:1396	The preventive effect of 2'-FL, scGOS/lcFOS, and their combination on dysbiosis associated to RV diarrhea in rats could be due to changes in the crosstalk between gut microbiota and the innate immune system.
31405262	8	60	theme	combination	1339:1349	arg1	due					1407:1409	due	1407:1409	due	1407:1409	The preventive effect of 2'-FL, scGOS/lcFOS, and their combination on dysbiosis associated to RV diarrhea in rats could be due to changes in the crosstalk between gut microbiota and the innate immune system.
31405262	2	61	from	diarrhea	459:466	arg1	rats					480:483	neonatal rats	471:483	neonatal rats	471:483	The present study aimed to investigate the effects of 2'-fucosyllactose (2'-FL), a mixture of short-chain galactooligosaccharides/long-chain fructooligosaccharides (scGOS/lcFOS) 9:1 and their combination (scGOS/lcFOS/2'-FL) on dysbiosis induced during rotavirus (RV) diarrhea in neonatal rats, elucidating crosstalk between bacteria and the immune system.
31405262	2	62	theme	2'-fucosyllactose	246:262	arg1	effects					235:241	the effects	231:241	the effects of 2'-fucosyllactose (2'-FL), a mixture of short-chain galactooligosaccharides/long-chain fructooligosaccharides (scGOS/lcFOS) 9:1 and their combination (scGOS/lcFOS/2'-FL) on dysbiosis induced during rotavirus (RV) diarrhea in neonatal rats	231:483	The present study aimed to investigate the effects of 2'-fucosyllactose (2'-FL), a mixture of short-chain galactooligosaccharides/long-chain fructooligosaccharides (scGOS/lcFOS) 9:1 and their combination (scGOS/lcFOS/2'-FL) on dysbiosis induced during rotavirus (RV) diarrhea in neonatal rats, elucidating crosstalk between bacteria and the immune system.
31405262	8	63	from	effect	1299:1304	arg1	dysbiosis					1354:1362	dysbiosis	1354:1362	dysbiosis associated to RV diarrhea in rats	1354:1396	The preventive effect of 2'-FL, scGOS/lcFOS, and their combination on dysbiosis associated to RV diarrhea in rats could be due to changes in the crosstalk between gut microbiota and the innate immune system.
31405262	7	64	theme	noninfected	1264:1274	arg1	groups					1276:1281	the noninfected groups	1260:1281	the noninfected groups	1260:1281	RV-infected rats displayed an intestinal dysbiosis that was effectively prevented by the dietary interventions, and consequently, their microbiota was more similar to microbiota of the noninfected groups.
31405262	6	65	theme	TLR7	1022:1025	arg1	expressions					1027:1037	TLR5 and TLR7 expressions	1013:1037	TLR5 and TLR7 expressions	1013:1037	RV infection increased TLR2 expression, whereas 2'-FL boosted TLR5 and TLR7 expressions and scGOS/lcFOS increased that of TLR9.
29385708	8	0	theme	efficient	1460:1468	arg1	glycosylation					1470:1482	efficient glycosylation	1460:1482	efficient glycosylation of camel HSPA6	1460:1497	Our data suggest that efficient glycosylation of camel HSPA6 is among the mechanisms that provide camelids with a superior capability for alleviating stressful environmental circumstances.
29385708	7	1	theme	stress	1419:1424	arg1	conditions					1426:1435	similar thermal and hypoxic stress conditions	1391:1435	conditions	1426:1435	Interestingly, the glycoform of camel HSPA6 was rapidly formed and stabilized under normal and stress culture conditions whereas human HSPA6 reacted differently under similar thermal and hypoxic stress conditions.
29385708	1	2	theme	shock	148:152	arg1	HSPs					164:167	HSPs	164:167	HSPs	164:167	Increased expression of heat shock proteins (HSPs) following heat stress or other stress conditions is a common physiological response in almost all living organisms.
29385708	1	2	theme	shock	148:152	arg1	proteins					154:161	heat shock proteins	143:161	heat shock proteins (HSPs)	143:168	Increased expression of heat shock proteins (HSPs) following heat stress or other stress conditions is a common physiological response in almost all living organisms.
29385708	4	3	theme	inducible	735:743	arg1	HSPA6					758:762	HSPA6	758:762	HSPA6	758:762	In this study, we cloned the cDNA encoding two inducible HSP members, HSPA6 and CRYAB from both camel (Camelus dromedarius) and human in a Myc-tagged mammalian expression vector.
29385708	4	3	theme	inducible	735:743	arg1	members					749:755	two inducible HSP members	731:755	two inducible HSP members	731:755	In this study, we cloned the cDNA encoding two inducible HSP members, HSPA6 and CRYAB from both camel (Camelus dromedarius) and human in a Myc-tagged mammalian expression vector.
29385708	4	3	theme	inducible	735:743	arg1	CRYAB					768:772	CRYAB	768:772	CRYAB	768:772	In this study, we cloned the cDNA encoding two inducible HSP members, HSPA6 and CRYAB from both camel (Camelus dromedarius) and human in a Myc-tagged mammalian expression vector.
29385708	1	4	theme	common	224:229	arg1	expression					129:138	Increased expression	119:138	Increased expression of heat shock proteins (HSPs) following heat stress or other stress conditions	119:217	Increased expression of heat shock proteins (HSPs) following heat stress or other stress conditions is a common physiological response in almost all living organisms.
29385708	1	4	theme	common	224:229	arg1	response					245:252	a common physiological response	222:252	a common physiological response in almost all living organisms	222:283	Increased expression of heat shock proteins (HSPs) following heat stress or other stress conditions is a common physiological response in almost all living organisms.
29385708	0	5	from	Glycosylation	13:25	arg1	Response					77:84	Response	77:84	Response to Thermal and Hypoxic Stresses	77:116	Differential Glycosylation and Modulation of Camel and Human HSP Isoforms in Response to Thermal and Hypoxic Stresses.
29385708	1	6	theme	proteins	154:161	arg1	expression					129:138	Increased expression	119:138	Increased expression of heat shock proteins (HSPs) following heat stress or other stress conditions	119:217	Increased expression of heat shock proteins (HSPs) following heat stress or other stress conditions is a common physiological response in almost all living organisms.
29385708	1	6	theme	proteins	154:161	arg1	response					245:252	a common physiological response	222:252	a common physiological response in almost all living organisms	222:283	Increased expression of heat shock proteins (HSPs) following heat stress or other stress conditions is a common physiological response in almost all living organisms.
29385708	1	7	theme	physiological	231:243	arg1	expression					129:138	Increased expression	119:138	Increased expression of heat shock proteins (HSPs) following heat stress or other stress conditions	119:217	Increased expression of heat shock proteins (HSPs) following heat stress or other stress conditions is a common physiological response in almost all living organisms.
29385708	1	7	theme	physiological	231:243	arg1	response					245:252	a common physiological response	222:252	a common physiological response in almost all living organisms	222:283	Increased expression of heat shock proteins (HSPs) following heat stress or other stress conditions is a common physiological response in almost all living organisms.
29385708	0	8	theme	Thermal	89:95	arg1	Stresses					109:116	Thermal and Hypoxic Stresses	89:116	Thermal and Hypoxic Stresses	89:116	Differential Glycosylation and Modulation of Camel and Human HSP Isoforms in Response to Thermal and Hypoxic Stresses.
29385708	5	9	theme	25-kDa	953:958	arg1	bands					930:934	protein bands	922:934	protein bands of approximately 25-kDa for both camel and human CRYAB and 70-kDa for camel HSPA6 and its human homologue	922:1040	Expression of these chaperones in COS-1 cells revealed protein bands of approximately 25-kDa for both camel and human CRYAB and 70-kDa for camel HSPA6 and its human homologue.
29385708	7	10	theme	stress	1319:1324	arg1	conditions					1334:1343	normal and stress culture conditions	1308:1343	conditions	1334:1343	Interestingly, the glycoform of camel HSPA6 was rapidly formed and stabilized under normal and stress culture conditions whereas human HSPA6 reacted differently under similar thermal and hypoxic stress conditions.
29385708	5	11	theme	human	979:983	arg1	CRYAB					985:989	human CRYAB	979:989	human CRYAB	979:989	Expression of these chaperones in COS-1 cells revealed protein bands of approximately 25-kDa for both camel and human CRYAB and 70-kDa for camel HSPA6 and its human homologue.
29385708	7	12	theme	HSPA6	1262:1266	arg1	glycoform					1243:1251	the glycoform	1239:1251	the glycoform of camel HSPA6	1239:1266	Interestingly, the glycoform of camel HSPA6 was rapidly formed and stabilized under normal and stress culture conditions whereas human HSPA6 reacted differently under similar thermal and hypoxic stress conditions.
29385708	6	13	theme	camel	1085:1089	arg1	HSPs					1101:1104	the camel and human HSPs	1081:1104	the camel and human HSPs	1081:1104	While localization and trafficking of the camel and human HSPs revealed similar cytosolic localization, we could demonstrate altered glycan structure between camel and human HSPA6.
29385708	5	14	theme	chaperones	887:896	arg1	Expression					867:876	Expression	867:876	Expression of these chaperones in COS-1 cells	867:911	Expression of these chaperones in COS-1 cells revealed protein bands of approximately 25-kDa for both camel and human CRYAB and 70-kDa for camel HSPA6 and its human homologue.
29385708	6	15	theme	cytosolic	1123:1131	arg1	localization					1133:1144	similar cytosolic localization	1115:1144	similar cytosolic localization	1115:1144	While localization and trafficking of the camel and human HSPs revealed similar cytosolic localization, we could demonstrate altered glycan structure between camel and human HSPA6.
29385708	5	16	theme	protein	922:928	arg1	bands					930:934	protein bands	922:934	protein bands of approximately 25-kDa for both camel and human CRYAB and 70-kDa for camel HSPA6 and its human homologue	922:1040	Expression of these chaperones in COS-1 cells revealed protein bands of approximately 25-kDa for both camel and human CRYAB and 70-kDa for camel HSPA6 and its human homologue.
29385708	0	17	theme	Hypoxic	101:107	arg1	Stresses					109:116	Thermal and Hypoxic Stresses	89:116	Thermal and Hypoxic Stresses	89:116	Differential Glycosylation and Modulation of Camel and Human HSP Isoforms in Response to Thermal and Hypoxic Stresses.
29385708	8	18	theme	HSPA6	1493:1497	arg1	glycosylation					1470:1482	efficient glycosylation	1460:1482	efficient glycosylation of camel HSPA6	1460:1497	Our data suggest that efficient glycosylation of camel HSPA6 is among the mechanisms that provide camelids with a superior capability for alleviating stressful environmental circumstances.
29385708	3	19	theme	key	460:462	arg1	HSPs					451:454	HSPs	451:454	HSPs	451:454	Since HSPs are key players in stress resistance and protein homeostasis, we aimed to analyze their forms at the cellular and molecular level using camel and human HSPs as models for efficient and moderate thermotolerant mammals, respectively.
29385708	3	19	theme	key	460:462	arg1	players					464:470	key players	460:470	key players in stress resistance and protein homeostasis	460:515	Since HSPs are key players in stress resistance and protein homeostasis, we aimed to analyze their forms at the cellular and molecular level using camel and human HSPs as models for efficient and moderate thermotolerant mammals, respectively.
29385708	4	20	theme	HSP	745:747	arg1	HSPA6					758:762	HSPA6	758:762	HSPA6	758:762	In this study, we cloned the cDNA encoding two inducible HSP members, HSPA6 and CRYAB from both camel (Camelus dromedarius) and human in a Myc-tagged mammalian expression vector.
29385708	4	20	theme	HSP	745:747	arg1	members					749:755	two inducible HSP members	731:755	two inducible HSP members	731:755	In this study, we cloned the cDNA encoding two inducible HSP members, HSPA6 and CRYAB from both camel (Camelus dromedarius) and human in a Myc-tagged mammalian expression vector.
29385708	4	20	theme	HSP	745:747	arg1	CRYAB					768:772	CRYAB	768:772	CRYAB	768:772	In this study, we cloned the cDNA encoding two inducible HSP members, HSPA6 and CRYAB from both camel (Camelus dromedarius) and human in a Myc-tagged mammalian expression vector.
29385708	7	21	theme	hypoxic	1411:1417	arg1	conditions					1426:1435	similar thermal and hypoxic stress conditions	1391:1435	conditions	1426:1435	Interestingly, the glycoform of camel HSPA6 was rapidly formed and stabilized under normal and stress culture conditions whereas human HSPA6 reacted differently under similar thermal and hypoxic stress conditions.
29385708	6	22	theme	human	1211:1215	arg1	HSPA6					1217:1221	human HSPA6	1211:1221	human HSPA6	1211:1221	While localization and trafficking of the camel and human HSPs revealed similar cytosolic localization, we could demonstrate altered glycan structure between camel and human HSPA6.
29385708	3	23	from	players	464:470	arg1	resistance					482:491	stress resistance	475:491	stress resistance	475:491	Since HSPs are key players in stress resistance and protein homeostasis, we aimed to analyze their forms at the cellular and molecular level using camel and human HSPs as models for efficient and moderate thermotolerant mammals, respectively.
29385708	3	23	from	players	464:470	arg1	homeostasis					505:515	protein homeostasis	497:515	protein homeostasis	497:515	Since HSPs are key players in stress resistance and protein homeostasis, we aimed to analyze their forms at the cellular and molecular level using camel and human HSPs as models for efficient and moderate thermotolerant mammals, respectively.
29385708	0	24	theme	Differential	0:11	arg1	Glycosylation					13:25	Differential Glycosylation	0:25	Differential Glycosylation	0:25	Differential Glycosylation and Modulation of Camel and Human HSP Isoforms in Response to Thermal and Hypoxic Stresses.
29385708	6	25	theme	HSPs	1101:1104	arg1	trafficking					1066:1076	trafficking	1066:1076	trafficking	1066:1076	While localization and trafficking of the camel and human HSPs revealed similar cytosolic localization, we could demonstrate altered glycan structure between camel and human HSPA6.
29385708	6	25	theme	HSPs	1101:1104	arg1	localization					1049:1060	localization	1049:1060	localization	1049:1060	While localization and trafficking of the camel and human HSPs revealed similar cytosolic localization, we could demonstrate altered glycan structure between camel and human HSPA6.
29385708	2	26	theme	cytosolic	302:310	arg1	HSPs					331:334	HSPs	331:334	HSPs	331:334	Modification of cytosolic proteins including HSPs by O-GlcNAc has been shown to enhance their capabilities for counteracting lethal levels of cellular stress.
29385708	2	26	theme	cytosolic	302:310	arg1	proteins					312:319	cytosolic proteins	302:319	cytosolic proteins including HSPs	302:334	Modification of cytosolic proteins including HSPs by O-GlcNAc has been shown to enhance their capabilities for counteracting lethal levels of cellular stress.
29385708	2	27	theme	stress	437:442	arg1	levels					418:423	lethal levels	411:423	lethal levels of cellular stress	411:442	Modification of cytosolic proteins including HSPs by O-GlcNAc has been shown to enhance their capabilities for counteracting lethal levels of cellular stress.
29385708	6	28	theme	human	1095:1099	arg1	HSPs					1101:1104	the camel and human HSPs	1081:1104	the camel and human HSPs	1081:1104	While localization and trafficking of the camel and human HSPs revealed similar cytosolic localization, we could demonstrate altered glycan structure between camel and human HSPA6.
29385708	2	29	theme	cellular	428:435	arg1	stress					437:442	cellular stress	428:442	cellular stress	428:442	Modification of cytosolic proteins including HSPs by O-GlcNAc has been shown to enhance their capabilities for counteracting lethal levels of cellular stress.
29385708	1	30	from	response	245:252	arg1	organisms					275:283	almost all living organisms	257:283	almost all living organisms	257:283	Increased expression of heat shock proteins (HSPs) following heat stress or other stress conditions is a common physiological response in almost all living organisms.
29385708	5	31	from	Expression	867:876	arg1	cells					907:911	COS-1 cells	901:911	COS-1 cells	901:911	Expression of these chaperones in COS-1 cells revealed protein bands of approximately 25-kDa for both camel and human CRYAB and 70-kDa for camel HSPA6 and its human homologue.
29385708	5	32	theme	COS-1	901:905	arg1	cells					907:911	COS-1 cells	901:911	COS-1 cells	901:911	Expression of these chaperones in COS-1 cells revealed protein bands of approximately 25-kDa for both camel and human CRYAB and 70-kDa for camel HSPA6 and its human homologue.
29385708	8	33	with	camelids	1536:1543	arg1	capability					1561:1570	a superior capability	1550:1570	a superior capability for alleviating stressful environmental circumstances	1550:1624	Our data suggest that efficient glycosylation of camel HSPA6 is among the mechanisms that provide camelids with a superior capability for alleviating stressful environmental circumstances.
29385708	1	34	theme	heat	180:183	arg1	stress					185:190	heat stress	180:190	heat stress	180:190	Increased expression of heat shock proteins (HSPs) following heat stress or other stress conditions is a common physiological response in almost all living organisms.
29385708	1	35	theme	living	268:273	arg1	organisms					275:283	almost all living organisms	257:283	almost all living organisms	257:283	Increased expression of heat shock proteins (HSPs) following heat stress or other stress conditions is a common physiological response in almost all living organisms.
29385708	6	36	theme	similar	1115:1121	arg1	localization					1133:1144	similar cytosolic localization	1115:1144	similar cytosolic localization	1115:1144	While localization and trafficking of the camel and human HSPs revealed similar cytosolic localization, we could demonstrate altered glycan structure between camel and human HSPA6.
29385708	3	37	theme	protein	497:503	arg1	homeostasis					505:515	protein homeostasis	497:515	protein homeostasis	497:515	Since HSPs are key players in stress resistance and protein homeostasis, we aimed to analyze their forms at the cellular and molecular level using camel and human HSPs as models for efficient and moderate thermotolerant mammals, respectively.
29385708	2	38	theme	lethal	411:416	arg1	levels					418:423	lethal levels	411:423	lethal levels of cellular stress	411:442	Modification of cytosolic proteins including HSPs by O-GlcNAc has been shown to enhance their capabilities for counteracting lethal levels of cellular stress.
29385708	0	39	theme	Camel	45:49	arg1	Isoforms					65:72	Camel and Human HSP Isoforms	45:72	Camel and Human HSP Isoforms	45:72	Differential Glycosylation and Modulation of Camel and Human HSP Isoforms in Response to Thermal and Hypoxic Stresses.
29385708	6	40	theme	glycan	1176:1181	arg1	structure					1183:1191	altered glycan structure	1168:1191	altered glycan structure between camel and human HSPA6	1168:1221	While localization and trafficking of the camel and human HSPs revealed similar cytosolic localization, we could demonstrate altered glycan structure between camel and human HSPA6.
29385708	3	41	theme	molecular	570:578	arg1	level					580:584	the cellular and molecular level	553:584	the cellular and molecular level	553:584	Since HSPs are key players in stress resistance and protein homeostasis, we aimed to analyze their forms at the cellular and molecular level using camel and human HSPs as models for efficient and moderate thermotolerant mammals, respectively.
29385708	3	42	from	level	580:584	arg1	forms					544:548	their forms	538:548	their forms at the cellular and molecular level	538:584	Since HSPs are key players in stress resistance and protein homeostasis, we aimed to analyze their forms at the cellular and molecular level using camel and human HSPs as models for efficient and moderate thermotolerant mammals, respectively.
29385708	7	43	gly	glycoform	1243:1251	arg1	HSPA6					1262:1266	camel HSPA6	1256:1266	camel HSPA6	1256:1266	Interestingly, the glycoform of camel HSPA6 was rapidly formed and stabilized under normal and stress culture conditions whereas human HSPA6 reacted differently under similar thermal and hypoxic stress conditions.
29385708	0	44	gly	Glycosylation	13:25	arg1	Isoforms					65:72	Camel and Human HSP Isoforms	45:72	Camel and Human HSP Isoforms	45:72	Differential Glycosylation and Modulation of Camel and Human HSP Isoforms in Response to Thermal and Hypoxic Stresses.
29385708	7	45	theme	culture	1326:1332	arg1	conditions					1334:1343	normal and stress culture conditions	1308:1343	conditions	1334:1343	Interestingly, the glycoform of camel HSPA6 was rapidly formed and stabilized under normal and stress culture conditions whereas human HSPA6 reacted differently under similar thermal and hypoxic stress conditions.
29385708	1	46	theme	other	195:199	arg1	conditions					208:217	other stress conditions	195:217	other stress conditions	195:217	Increased expression of heat shock proteins (HSPs) following heat stress or other stress conditions is a common physiological response in almost all living organisms.
29385708	0	47	theme	Human	55:59	arg1	Isoforms					65:72	Camel and Human HSP Isoforms	45:72	Camel and Human HSP Isoforms	45:72	Differential Glycosylation and Modulation of Camel and Human HSP Isoforms in Response to Thermal and Hypoxic Stresses.
29385708	4	48	theme	mammalian	838:846	arg1	vector					859:864	a Myc-tagged mammalian expression vector	825:864	a Myc-tagged mammalian expression vector	825:864	In this study, we cloned the cDNA encoding two inducible HSP members, HSPA6 and CRYAB from both camel (Camelus dromedarius) and human in a Myc-tagged mammalian expression vector.
29385708	5	49	theme	human	1026:1030	arg1	homologue					1032:1040	its human homologue	1022:1040	its human homologue	1022:1040	Expression of these chaperones in COS-1 cells revealed protein bands of approximately 25-kDa for both camel and human CRYAB and 70-kDa for camel HSPA6 and its human homologue.
29385708	3	50	theme	cellular	557:564	arg1	level					580:584	the cellular and molecular level	553:584	the cellular and molecular level	553:584	Since HSPs are key players in stress resistance and protein homeostasis, we aimed to analyze their forms at the cellular and molecular level using camel and human HSPs as models for efficient and moderate thermotolerant mammals, respectively.
29385708	3	51	theme	stress	475:480	arg1	resistance					482:491	stress resistance	475:491	stress resistance	475:491	Since HSPs are key players in stress resistance and protein homeostasis, we aimed to analyze their forms at the cellular and molecular level using camel and human HSPs as models for efficient and moderate thermotolerant mammals, respectively.
29385708	4	52	theme	Myc-tagged	827:836	arg1	vector					859:864	a Myc-tagged mammalian expression vector	825:864	a Myc-tagged mammalian expression vector	825:864	In this study, we cloned the cDNA encoding two inducible HSP members, HSPA6 and CRYAB from both camel (Camelus dromedarius) and human in a Myc-tagged mammalian expression vector.
29385708	3	53	theme	moderate	641:648	arg1	mammals					665:671	efficient and moderate thermotolerant mammals	627:671	efficient and moderate thermotolerant mammals	627:671	Since HSPs are key players in stress resistance and protein homeostasis, we aimed to analyze their forms at the cellular and molecular level using camel and human HSPs as models for efficient and moderate thermotolerant mammals, respectively.
29385708	5	54	theme	70-kDa	995:1000	arg1	bands					930:934	protein bands	922:934	protein bands of approximately 25-kDa for both camel and human CRYAB and 70-kDa for camel HSPA6 and its human homologue	922:1040	Expression of these chaperones in COS-1 cells revealed protein bands of approximately 25-kDa for both camel and human CRYAB and 70-kDa for camel HSPA6 and its human homologue.
29385708	8	55	gly	glycosylation	1470:1482	arg1	HSPA6					1493:1497	camel HSPA6	1487:1497	camel HSPA6	1487:1497	Our data suggest that efficient glycosylation of camel HSPA6 is among the mechanisms that provide camelids with a superior capability for alleviating stressful environmental circumstances.
29385708	6	56	theme	altered	1168:1174	arg1	structure					1183:1191	altered glycan structure	1168:1191	altered glycan structure between camel and human HSPA6	1168:1221	While localization and trafficking of the camel and human HSPs revealed similar cytosolic localization, we could demonstrate altered glycan structure between camel and human HSPA6.
29385708	8	57	theme	superior	1552:1559	arg1	capability					1561:1570	a superior capability	1550:1570	a superior capability for alleviating stressful environmental circumstances	1550:1624	Our data suggest that efficient glycosylation of camel HSPA6 is among the mechanisms that provide camelids with a superior capability for alleviating stressful environmental circumstances.
29385708	3	58	theme	thermotolerant	650:663	arg1	mammals					665:671	efficient and moderate thermotolerant mammals	627:671	efficient and moderate thermotolerant mammals	627:671	Since HSPs are key players in stress resistance and protein homeostasis, we aimed to analyze their forms at the cellular and molecular level using camel and human HSPs as models for efficient and moderate thermotolerant mammals, respectively.
29385708	7	59	theme	normal	1308:1313	arg1	conditions					1334:1343	normal and stress culture conditions	1308:1343	conditions	1334:1343	Interestingly, the glycoform of camel HSPA6 was rapidly formed and stabilized under normal and stress culture conditions whereas human HSPA6 reacted differently under similar thermal and hypoxic stress conditions.
29385708	1	60	theme	Increased	119:127	arg1	expression					129:138	Increased expression	119:138	Increased expression of heat shock proteins (HSPs) following heat stress or other stress conditions	119:217	Increased expression of heat shock proteins (HSPs) following heat stress or other stress conditions is a common physiological response in almost all living organisms.
29385708	1	60	theme	Increased	119:127	arg1	response					245:252	a common physiological response	222:252	a common physiological response in almost all living organisms	222:283	Increased expression of heat shock proteins (HSPs) following heat stress or other stress conditions is a common physiological response in almost all living organisms.
29385708	7	61	theme	human	1353:1357	arg1	HSPA6					1359:1363	human HSPA6	1353:1363	human HSPA6	1353:1363	Interestingly, the glycoform of camel HSPA6 was rapidly formed and stabilized under normal and stress culture conditions whereas human HSPA6 reacted differently under similar thermal and hypoxic stress conditions.
29385708	1	62	theme	stress	201:206	arg1	conditions					208:217	other stress conditions	195:217	other stress conditions	195:217	Increased expression of heat shock proteins (HSPs) following heat stress or other stress conditions is a common physiological response in almost all living organisms.
29385708	4	63	theme	expression	848:857	arg1	vector					859:864	a Myc-tagged mammalian expression vector	825:864	a Myc-tagged mammalian expression vector	825:864	In this study, we cloned the cDNA encoding two inducible HSP members, HSPA6 and CRYAB from both camel (Camelus dromedarius) and human in a Myc-tagged mammalian expression vector.
29385708	3	64	theme	efficient	627:635	arg1	mammals					665:671	efficient and moderate thermotolerant mammals	627:671	efficient and moderate thermotolerant mammals	627:671	Since HSPs are key players in stress resistance and protein homeostasis, we aimed to analyze their forms at the cellular and molecular level using camel and human HSPs as models for efficient and moderate thermotolerant mammals, respectively.
29385708	3	65	theme	human	602:606	arg1	models					616:621	models	616:621	models for efficient and moderate thermotolerant mammals	616:671	Since HSPs are key players in stress resistance and protein homeostasis, we aimed to analyze their forms at the cellular and molecular level using camel and human HSPs as models for efficient and moderate thermotolerant mammals, respectively.
29385708	3	65	theme	human	602:606	arg1	HSPs					608:611	camel and human HSPs	592:611	camel and human HSPs	592:611	Since HSPs are key players in stress resistance and protein homeostasis, we aimed to analyze their forms at the cellular and molecular level using camel and human HSPs as models for efficient and moderate thermotolerant mammals, respectively.
29385708	2	66	theme	proteins	312:319	arg1	Modification					286:297	Modification	286:297	Modification of cytosolic proteins including HSPs by O-GlcNAc	286:346	Modification of cytosolic proteins including HSPs by O-GlcNAc has been shown to enhance their capabilities for counteracting lethal levels of cellular stress.
29385708	0	67	theme	Isoforms	65:72	arg1	Modulation					31:40	Modulation	31:40	Modulation	31:40	Differential Glycosylation and Modulation of Camel and Human HSP Isoforms in Response to Thermal and Hypoxic Stresses.
29385708	0	67	theme	Isoforms	65:72	arg1	Glycosylation					13:25	Differential Glycosylation	0:25	Differential Glycosylation	0:25	Differential Glycosylation and Modulation of Camel and Human HSP Isoforms in Response to Thermal and Hypoxic Stresses.
29385708	5	68	theme	camel	1006:1010	arg1	HSPA6					1012:1016	camel HSPA6	1006:1016	camel HSPA6	1006:1016	Expression of these chaperones in COS-1 cells revealed protein bands of approximately 25-kDa for both camel and human CRYAB and 70-kDa for camel HSPA6 and its human homologue.
29385708	4	69	dep	camel	784:788	arg1	dromedarius					799:809	Camelus dromedarius	791:809	Camelus dromedarius	791:809	In this study, we cloned the cDNA encoding two inducible HSP members, HSPA6 and CRYAB from both camel (Camelus dromedarius) and human in a Myc-tagged mammalian expression vector.
29385708	7	70	theme	camel	1256:1260	arg1	HSPA6					1262:1266	camel HSPA6	1256:1266	camel HSPA6	1256:1266	Interestingly, the glycoform of camel HSPA6 was rapidly formed and stabilized under normal and stress culture conditions whereas human HSPA6 reacted differently under similar thermal and hypoxic stress conditions.
29385708	8	71	theme	environmental	1598:1610	arg1	circumstances					1612:1624	stressful environmental circumstances	1588:1624	stressful environmental circumstances	1588:1624	Our data suggest that efficient glycosylation of camel HSPA6 is among the mechanisms that provide camelids with a superior capability for alleviating stressful environmental circumstances.
29385708	0	72	theme	HSP	61:63	arg1	Isoforms					65:72	Camel and Human HSP Isoforms	45:72	Camel and Human HSP Isoforms	45:72	Differential Glycosylation and Modulation of Camel and Human HSP Isoforms in Response to Thermal and Hypoxic Stresses.
29385708	0	73	from	Modulation	31:40	arg1	Response					77:84	Response	77:84	Response to Thermal and Hypoxic Stresses	77:116	Differential Glycosylation and Modulation of Camel and Human HSP Isoforms in Response to Thermal and Hypoxic Stresses.
29385708	3	74	theme	camel	592:596	arg1	models					616:621	models	616:621	models for efficient and moderate thermotolerant mammals	616:671	Since HSPs are key players in stress resistance and protein homeostasis, we aimed to analyze their forms at the cellular and molecular level using camel and human HSPs as models for efficient and moderate thermotolerant mammals, respectively.
29385708	3	74	theme	camel	592:596	arg1	HSPs					608:611	camel and human HSPs	592:611	camel and human HSPs	592:611	Since HSPs are key players in stress resistance and protein homeostasis, we aimed to analyze their forms at the cellular and molecular level using camel and human HSPs as models for efficient and moderate thermotolerant mammals, respectively.
29385708	1	75	theme	heat	143:146	arg1	HSPs					164:167	HSPs	164:167	HSPs	164:167	Increased expression of heat shock proteins (HSPs) following heat stress or other stress conditions is a common physiological response in almost all living organisms.
29385708	1	75	theme	heat	143:146	arg1	proteins					154:161	heat shock proteins	143:161	heat shock proteins (HSPs)	143:168	Increased expression of heat shock proteins (HSPs) following heat stress or other stress conditions is a common physiological response in almost all living organisms.
29385708	8	76	theme	camel	1487:1491	arg1	HSPA6					1493:1497	camel HSPA6	1487:1497	camel HSPA6	1487:1497	Our data suggest that efficient glycosylation of camel HSPA6 is among the mechanisms that provide camelids with a superior capability for alleviating stressful environmental circumstances.
29385708	8	77	theme	stressful	1588:1596	arg1	circumstances					1612:1624	stressful environmental circumstances	1588:1624	stressful environmental circumstances	1588:1624	Our data suggest that efficient glycosylation of camel HSPA6 is among the mechanisms that provide camelids with a superior capability for alleviating stressful environmental circumstances.
29697853	0	0	theme	bone	93:96	arg1	formation					98:106	bone formation	93:106	bone formation	93:106	Injectable BMP-2 delivery system based on collagen-derived microspheres and alginate induced bone formation in a time- and dose-dependent manner.
29697853	4	1	theme	micro-computed	909:922	arg1	µCT					936:938	µCT	936:938	µCT	936:938	BMP-2 doses of 10 µg, 3 µg and 1 µg per implant (50 µg/mL, 15 µg/mL and 5 µg/mL, respectively) successfully induced ectopic bone formation subcutaneously in rats in a time- and dose-dependent manner, as shown by micro-computed tomography (µCT) and histology.
29697853	4	1	theme	micro-computed	909:922	arg1	tomography					924:933	micro-computed tomography	909:933	micro-computed tomography (µCT)	909:939	BMP-2 doses of 10 µg, 3 µg and 1 µg per implant (50 µg/mL, 15 µg/mL and 5 µg/mL, respectively) successfully induced ectopic bone formation subcutaneously in rats in a time- and dose-dependent manner, as shown by micro-computed tomography (µCT) and histology.
29697853	6	2	theme	BMP-2	1183:1187	arg1	µg					1177:1178	the 2 µg	1171:1178	the 2 µg of BMP-2	1171:1187	In the subcritical calvarial defect model, µCT revealed a higher bone volume for the 2 µg of BMP-2 per implant condition (50 µg/mL) as compared to the lower dose used (0.2 µg per implant, 5 µg/mL).
29697853	1	3	theme	morphogenetic	242:254	arg1	protein-2					256:264	bone morphogenetic protein-2	237:264	bone morphogenetic protein-2 (BMP-2) (usually 1.5 mg/mL)	237:292	The aim of the current study was to reduce the clinically used supra-physiological dose of bone morphogenetic protein-2 (BMP-2) (usually 1.5 mg/mL), which carries the risk of adverse events, by using a more effective release system.
29697853	1	3	theme	morphogenetic	242:254	arg1	mg/mL					287:291	usually 1.5 mg/mL	275:291	usually 1.5 mg/mL	275:291	The aim of the current study was to reduce the clinically used supra-physiological dose of bone morphogenetic protein-2 (BMP-2) (usually 1.5 mg/mL), which carries the risk of adverse events, by using a more effective release system.
29697853	1	3	theme	morphogenetic	242:254	arg1	BMP-2					267:271	BMP-2	267:271	BMP-2	267:271	The aim of the current study was to reduce the clinically used supra-physiological dose of bone morphogenetic protein-2 (BMP-2) (usually 1.5 mg/mL), which carries the risk of adverse events, by using a more effective release system.
29697853	7	4	theme	µg/mL	1334:1338	arg1	BMP-2					1340:1344	50 µg/mL BMP-2	1331:1344	50 µg/mL BMP-2	1331:1344	Complete defect bridging was obtained with 50 µg/mL BMP-2 after 8 weeks.
29697853	9	5	theme	µg/mL	1589:1593	arg1	BMP-2					1595:1599	50 µg/mL BMP-2	1586:1599	50 µg/mL BMP-2 in both the ectopic and calvarial rat defect models	1586:1651	Overall, this injectable BMP-2 delivery system showed promising results with 50 µg/mL BMP-2 in both the ectopic and calvarial rat defect models, underling the potential of this composite hydrogel for bone regeneration therapies.
29697853	3	6	theme	bone	623:626	arg1	capacity					641:648	bone regeneration capacity	623:648	bone regeneration capacity	623:648	Time- and dose-dependent subcutaneous ectopic bone formation within this system and bone regeneration capacity in a calvarial defect model were investigated.
29697853	9	7	theme	bone	1709:1712	arg1	therapies					1727:1735	bone regeneration therapies	1709:1735	bone regeneration therapies	1709:1735	Overall, this injectable BMP-2 delivery system showed promising results with 50 µg/mL BMP-2 in both the ectopic and calvarial rat defect models, underling the potential of this composite hydrogel for bone regeneration therapies.
29697853	1	8	theme	protein-2	256:264	arg1	dose					229:232	the clinically used supra-physiological dose	189:232	the clinically used supra-physiological dose of bone morphogenetic protein-2 (BMP-2) (usually 1.5 mg/mL), which carries the risk of adverse events	189:334	The aim of the current study was to reduce the clinically used supra-physiological dose of bone morphogenetic protein-2 (BMP-2) (usually 1.5 mg/mL), which carries the risk of adverse events, by using a more effective release system.
29697853	4	9	theme	dose-dependent	874:887	arg1	manner					889:894	dose-dependent manner	874:894	dose-dependent manner	874:894	BMP-2 doses of 10 µg, 3 µg and 1 µg per implant (50 µg/mL, 15 µg/mL and 5 µg/mL, respectively) successfully induced ectopic bone formation subcutaneously in rats in a time- and dose-dependent manner, as shown by micro-computed tomography (µCT) and histology.
29697853	4	10	theme	ectopic	813:819	arg1	formation					826:834	ectopic bone formation	813:834	ectopic bone formation	813:834	BMP-2 doses of 10 µg, 3 µg and 1 µg per implant (50 µg/mL, 15 µg/mL and 5 µg/mL, respectively) successfully induced ectopic bone formation subcutaneously in rats in a time- and dose-dependent manner, as shown by micro-computed tomography (µCT) and histology.
29697853	6	11	theme	subcritical	1097:1107	arg1	model					1126:1130	the subcritical calvarial defect model	1093:1130	the subcritical calvarial defect model	1093:1130	In the subcritical calvarial defect model, µCT revealed a higher bone volume for the 2 µg of BMP-2 per implant condition (50 µg/mL) as compared to the lower dose used (0.2 µg per implant, 5 µg/mL).
29697853	5	12	theme	retention	1006:1014	arg1	control					989:995	the spatio-temporal control	969:995	the spatio-temporal control of BMP-2 retention	969:1014	In addition, the spatio-temporal control of BMP-2 retention was shown for 4 weeks in vivo by imaging of fluorescently-labelled BMP-2.
29697853	1	13	theme	adverse	321:327	arg1	events					329:334	adverse events	321:334	adverse events	321:334	The aim of the current study was to reduce the clinically used supra-physiological dose of bone morphogenetic protein-2 (BMP-2) (usually 1.5 mg/mL), which carries the risk of adverse events, by using a more effective release system.
29697853	3	14	from	formation	590:598	arg1	model					672:676	a calvarial defect model	653:676	a calvarial defect model	653:676	Time- and dose-dependent subcutaneous ectopic bone formation within this system and bone regeneration capacity in a calvarial defect model were investigated.
29697853	8	15	theme	empty	1458:1462	arg1	defect					1464:1469	the empty defect	1454:1469	the empty defect	1454:1469	The BMP-2 concentration of 5 µg/mL was not sufficient to heal a calvarial defect faster than the empty defect or biomaterial control without BMP-2.
29697853	2	16	theme	collagen-based	471:484	arg1	microspheres					486:497	BMP-2-loaded recombinant collagen-based microspheres	446:497	BMP-2-loaded recombinant collagen-based microspheres	446:497	A slow release system, based on an injectable hydrogel composed of BMP-2-loaded recombinant collagen-based microspheres and alginate, was previously developed.
29697853	1	17	theme	events	329:334	arg1	risk					313:316	the risk	309:316	the risk of adverse events	309:334	The aim of the current study was to reduce the clinically used supra-physiological dose of bone morphogenetic protein-2 (BMP-2) (usually 1.5 mg/mL), which carries the risk of adverse events, by using a more effective release system.
29697853	6	18	dep	used	1252:1255	arg1	µg					1262:1263	0.2 µg	1258:1263	0.2 µg per implant	1258:1275	In the subcritical calvarial defect model, µCT revealed a higher bone volume for the 2 µg of BMP-2 per implant condition (50 µg/mL) as compared to the lower dose used (0.2 µg per implant, 5 µg/mL).
29697853	6	18	dep	used	1252:1255	arg1	dose					1247:1250	the lower dose	1237:1250	the lower dose used (0.2 µg per implant, 5 µg/mL)	1237:1285	In the subcritical calvarial defect model, µCT revealed a higher bone volume for the 2 µg of BMP-2 per implant condition (50 µg/mL) as compared to the lower dose used (0.2 µg per implant, 5 µg/mL).
29697853	9	19	theme	defect	1639:1644	arg1	models					1646:1651	rat defect models	1635:1651	rat defect models	1635:1651	Overall, this injectable BMP-2 delivery system showed promising results with 50 µg/mL BMP-2 in both the ectopic and calvarial rat defect models, underling the potential of this composite hydrogel for bone regeneration therapies.
29697853	1	20	theme	current	161:167	arg1	study					169:173	the current study	157:173	the current study	157:173	The aim of the current study was to reduce the clinically used supra-physiological dose of bone morphogenetic protein-2 (BMP-2) (usually 1.5 mg/mL), which carries the risk of adverse events, by using a more effective release system.
29697853	2	21	theme	recombinant	459:469	arg1	microspheres					486:497	BMP-2-loaded recombinant collagen-based microspheres	446:497	BMP-2-loaded recombinant collagen-based microspheres	446:497	A slow release system, based on an injectable hydrogel composed of BMP-2-loaded recombinant collagen-based microspheres and alginate, was previously developed.
29697853	7	22	theme	Complete	1288:1295	arg1	defect					1297:1302	Complete defect	1288:1302	Complete defect bridging	1288:1311	Complete defect bridging was obtained with 50 µg/mL BMP-2 after 8 weeks.
29697853	4	23	theme	µg	730:731	arg1	doses					703:707	BMP-2 doses	697:707	BMP-2 doses of 10 µg, 3 µg and 1 µg per implant (50 µg/mL, 15 µg/mL and 5 µg/mL, respectively)	697:790	BMP-2 doses of 10 µg, 3 µg and 1 µg per implant (50 µg/mL, 15 µg/mL and 5 µg/mL, respectively) successfully induced ectopic bone formation subcutaneously in rats in a time- and dose-dependent manner, as shown by micro-computed tomography (µCT) and histology.
29697853	1	24	theme	study	169:173	arg1	aim					150:152	The aim	146:152	The aim of the current study	146:173	The aim of the current study was to reduce the clinically used supra-physiological dose of bone morphogenetic protein-2 (BMP-2) (usually 1.5 mg/mL), which carries the risk of adverse events, by using a more effective release system.
29697853	0	25	theme	BMP-2	11:15	arg1	system					26:31	Injectable BMP-2 delivery system	0:31	Injectable BMP-2 delivery system based on collagen-derived microspheres and alginate	0:83	Injectable BMP-2 delivery system based on collagen-derived microspheres and alginate induced bone formation in a time- and dose-dependent manner.
29697853	8	26	theme	BMP-2	1365:1369	arg1	sufficient					1404:1413	sufficient	1404:1413	sufficient	1404:1413	The BMP-2 concentration of 5 µg/mL was not sufficient to heal a calvarial defect faster than the empty defect or biomaterial control without BMP-2.
29697853	8	26	theme	BMP-2	1365:1369	arg1	concentration					1371:1383	The BMP-2 concentration	1361:1383	The BMP-2 concentration of 5 µg/mL	1361:1394	The BMP-2 concentration of 5 µg/mL was not sufficient to heal a calvarial defect faster than the empty defect or biomaterial control without BMP-2.
29697853	0	27	link	collagen-derived	42:57	arg1	microspheres					59:70	collagen-derived microspheres	42:70	collagen-derived microspheres	42:70	Injectable BMP-2 delivery system based on collagen-derived microspheres and alginate induced bone formation in a time- and dose-dependent manner.
29697853	9	28	dep	ectopic	1613:1619	arg1	the					1609:1611	the	1609:1611	the	1609:1611	Overall, this injectable BMP-2 delivery system showed promising results with 50 µg/mL BMP-2 in both the ectopic and calvarial rat defect models, underling the potential of this composite hydrogel for bone regeneration therapies.
29697853	9	28	dep	ectopic	1613:1619	arg1	models					1646:1651	rat defect models	1635:1651	rat defect models	1635:1651	Overall, this injectable BMP-2 delivery system showed promising results with 50 µg/mL BMP-2 in both the ectopic and calvarial rat defect models, underling the potential of this composite hydrogel for bone regeneration therapies.
29697853	0	29	theme	Injectable	0:9	arg1	system					26:31	Injectable BMP-2 delivery system	0:31	Injectable BMP-2 delivery system based on collagen-derived microspheres and alginate	0:83	Injectable BMP-2 delivery system based on collagen-derived microspheres and alginate induced bone formation in a time- and dose-dependent manner.
29697853	6	30	theme	bone	1155:1158	arg1	volume					1160:1165	a higher bone volume	1146:1165	a higher bone volume for the 2 µg of BMP-2 per implant condition (50 µg/mL)	1146:1220	In the subcritical calvarial defect model, µCT revealed a higher bone volume for the 2 µg of BMP-2 per implant condition (50 µg/mL) as compared to the lower dose used (0.2 µg per implant, 5 µg/mL).
29697853	3	31	theme	calvarial	655:663	arg1	model					672:676	a calvarial defect model	653:676	a calvarial defect model	653:676	Time- and dose-dependent subcutaneous ectopic bone formation within this system and bone regeneration capacity in a calvarial defect model were investigated.
29697853	4	32	dep	implant	737:743	arg1	µg/mL					749:753	50 µg/mL	746:753	50 µg/mL	746:753	BMP-2 doses of 10 µg, 3 µg and 1 µg per implant (50 µg/mL, 15 µg/mL and 5 µg/mL, respectively) successfully induced ectopic bone formation subcutaneously in rats in a time- and dose-dependent manner, as shown by micro-computed tomography (µCT) and histology.
29697853	4	32	dep	implant	737:743	arg1	µg/mL					759:763	15 µg/mL and 5 µg/mL	756:775	µg/mL	759:763	BMP-2 doses of 10 µg, 3 µg and 1 µg per implant (50 µg/mL, 15 µg/mL and 5 µg/mL, respectively) successfully induced ectopic bone formation subcutaneously in rats in a time- and dose-dependent manner, as shown by micro-computed tomography (µCT) and histology.
29697853	4	32	dep	implant	737:743	arg1	µg/mL					771:775	15 µg/mL and 5 µg/mL	756:775	µg/mL	771:775	BMP-2 doses of 10 µg, 3 µg and 1 µg per implant (50 µg/mL, 15 µg/mL and 5 µg/mL, respectively) successfully induced ectopic bone formation subcutaneously in rats in a time- and dose-dependent manner, as shown by micro-computed tomography (µCT) and histology.
29697853	2	33	theme	release	386:392	arg1	system					394:399	A slow release system	379:399	A slow release system	379:399	A slow release system, based on an injectable hydrogel composed of BMP-2-loaded recombinant collagen-based microspheres and alginate, was previously developed.
29697853	4	34	theme	µg	715:716	arg1	doses					703:707	BMP-2 doses	697:707	BMP-2 doses of 10 µg, 3 µg and 1 µg per implant (50 µg/mL, 15 µg/mL and 5 µg/mL, respectively)	697:790	BMP-2 doses of 10 µg, 3 µg and 1 µg per implant (50 µg/mL, 15 µg/mL and 5 µg/mL, respectively) successfully induced ectopic bone formation subcutaneously in rats in a time- and dose-dependent manner, as shown by micro-computed tomography (µCT) and histology.
29697853	0	35	theme	dose-dependent	123:136	arg1	manner					138:143	dose-dependent manner	123:143	dose-dependent manner	123:143	Injectable BMP-2 delivery system based on collagen-derived microspheres and alginate induced bone formation in a time- and dose-dependent manner.
29697853	9	36	theme	rat	1635:1637	arg1	models					1646:1651	rat defect models	1635:1651	rat defect models	1635:1651	Overall, this injectable BMP-2 delivery system showed promising results with 50 µg/mL BMP-2 in both the ectopic and calvarial rat defect models, underling the potential of this composite hydrogel for bone regeneration therapies.
29697853	4	37	theme	µg	721:722	arg1	doses					703:707	BMP-2 doses	697:707	BMP-2 doses of 10 µg, 3 µg and 1 µg per implant (50 µg/mL, 15 µg/mL and 5 µg/mL, respectively)	697:790	BMP-2 doses of 10 µg, 3 µg and 1 µg per implant (50 µg/mL, 15 µg/mL and 5 µg/mL, respectively) successfully induced ectopic bone formation subcutaneously in rats in a time- and dose-dependent manner, as shown by micro-computed tomography (µCT) and histology.
29697853	6	38	theme	higher	1148:1153	arg1	volume					1160:1165	a higher bone volume	1146:1165	a higher bone volume for the 2 µg of BMP-2 per implant condition (50 µg/mL)	1146:1220	In the subcritical calvarial defect model, µCT revealed a higher bone volume for the 2 µg of BMP-2 per implant condition (50 µg/mL) as compared to the lower dose used (0.2 µg per implant, 5 µg/mL).
29697853	2	39	theme	slow	381:384	arg1	system					394:399	A slow release system	379:399	A slow release system	379:399	A slow release system, based on an injectable hydrogel composed of BMP-2-loaded recombinant collagen-based microspheres and alginate, was previously developed.
29697853	6	40	dep	µg	1262:1263	arg1	µg/mL					1280:1284	5 µg/mL	1278:1284	5 µg/mL	1278:1284	In the subcritical calvarial defect model, µCT revealed a higher bone volume for the 2 µg of BMP-2 per implant condition (50 µg/mL) as compared to the lower dose used (0.2 µg per implant, 5 µg/mL).
29697853	3	41	theme	regeneration	628:639	arg1	capacity					641:648	bone regeneration capacity	623:648	bone regeneration capacity	623:648	Time- and dose-dependent subcutaneous ectopic bone formation within this system and bone regeneration capacity in a calvarial defect model were investigated.
29697853	2	42	theme	injectable	414:423	arg1	hydrogel					425:432	an injectable hydrogel	411:432	an injectable hydrogel composed of BMP-2-loaded recombinant collagen-based microspheres and alginate	411:510	A slow release system, based on an injectable hydrogel composed of BMP-2-loaded recombinant collagen-based microspheres and alginate, was previously developed.
29697853	8	43	theme	µg/mL	1390:1394	arg1	sufficient					1404:1413	sufficient	1404:1413	sufficient	1404:1413	The BMP-2 concentration of 5 µg/mL was not sufficient to heal a calvarial defect faster than the empty defect or biomaterial control without BMP-2.
29697853	8	43	theme	µg/mL	1390:1394	arg1	concentration					1371:1383	The BMP-2 concentration	1361:1383	The BMP-2 concentration of 5 µg/mL	1361:1394	The BMP-2 concentration of 5 µg/mL was not sufficient to heal a calvarial defect faster than the empty defect or biomaterial control without BMP-2.
29697853	9	44	theme	BMP-2	1534:1538	arg1	system					1549:1554	this injectable BMP-2 delivery system	1518:1554	this injectable BMP-2 delivery system	1518:1554	Overall, this injectable BMP-2 delivery system showed promising results with 50 µg/mL BMP-2 in both the ectopic and calvarial rat defect models, underling the potential of this composite hydrogel for bone regeneration therapies.
29697853	3	45	theme	ectopic	577:583	arg1	formation					590:598	dose-dependent subcutaneous ectopic bone formation	549:598	dose-dependent subcutaneous ectopic bone formation within this system	549:617	Time- and dose-dependent subcutaneous ectopic bone formation within this system and bone regeneration capacity in a calvarial defect model were investigated.
29697853	6	46	theme	calvarial	1109:1117	arg1	model					1126:1130	the subcritical calvarial defect model	1093:1130	the subcritical calvarial defect model	1093:1130	In the subcritical calvarial defect model, µCT revealed a higher bone volume for the 2 µg of BMP-2 per implant condition (50 µg/mL) as compared to the lower dose used (0.2 µg per implant, 5 µg/mL).
29697853	8	47	theme	biomaterial	1474:1484	arg1	control					1486:1492	biomaterial control	1474:1492	biomaterial control without BMP-2	1474:1506	The BMP-2 concentration of 5 µg/mL was not sufficient to heal a calvarial defect faster than the empty defect or biomaterial control without BMP-2.
29697853	9	48	theme	delivery	1540:1547	arg1	system					1549:1554	this injectable BMP-2 delivery system	1518:1554	this injectable BMP-2 delivery system	1518:1554	Overall, this injectable BMP-2 delivery system showed promising results with 50 µg/mL BMP-2 in both the ectopic and calvarial rat defect models, underling the potential of this composite hydrogel for bone regeneration therapies.
29697853	5	49	theme	BMP-2	1000:1004	arg1	retention					1006:1014	BMP-2 retention	1000:1014	BMP-2 retention	1000:1014	In addition, the spatio-temporal control of BMP-2 retention was shown for 4 weeks in vivo by imaging of fluorescently-labelled BMP-2.
29697853	3	50	theme	bone	585:588	arg1	formation					590:598	dose-dependent subcutaneous ectopic bone formation	549:598	dose-dependent subcutaneous ectopic bone formation within this system	549:617	Time- and dose-dependent subcutaneous ectopic bone formation within this system and bone regeneration capacity in a calvarial defect model were investigated.
29697853	1	51	theme	used	204:207	arg1	dose					229:232	the clinically used supra-physiological dose	189:232	the clinically used supra-physiological dose of bone morphogenetic protein-2 (BMP-2) (usually 1.5 mg/mL), which carries the risk of adverse events	189:334	The aim of the current study was to reduce the clinically used supra-physiological dose of bone morphogenetic protein-2 (BMP-2) (usually 1.5 mg/mL), which carries the risk of adverse events, by using a more effective release system.
29697853	4	52	dep	time-	864:868	arg1	a					862:862	a	862:862	a	862:862	BMP-2 doses of 10 µg, 3 µg and 1 µg per implant (50 µg/mL, 15 µg/mL and 5 µg/mL, respectively) successfully induced ectopic bone formation subcutaneously in rats in a time- and dose-dependent manner, as shown by micro-computed tomography (µCT) and histology.
29697853	6	53	theme	lower	1241:1245	arg1	µg					1262:1263	0.2 µg	1258:1263	0.2 µg per implant	1258:1275	In the subcritical calvarial defect model, µCT revealed a higher bone volume for the 2 µg of BMP-2 per implant condition (50 µg/mL) as compared to the lower dose used (0.2 µg per implant, 5 µg/mL).
29697853	6	53	theme	lower	1241:1245	arg1	dose					1247:1250	the lower dose	1237:1250	the lower dose used (0.2 µg per implant, 5 µg/mL)	1237:1285	In the subcritical calvarial defect model, µCT revealed a higher bone volume for the 2 µg of BMP-2 per implant condition (50 µg/mL) as compared to the lower dose used (0.2 µg per implant, 5 µg/mL).
29697853	1	54	theme	effective	353:361	arg1	system					371:376	a more effective release system	346:376	a more effective release system	346:376	The aim of the current study was to reduce the clinically used supra-physiological dose of bone morphogenetic protein-2 (BMP-2) (usually 1.5 mg/mL), which carries the risk of adverse events, by using a more effective release system.
29697853	3	55	theme	dose-dependent	549:562	arg1	formation					590:598	dose-dependent subcutaneous ectopic bone formation	549:598	dose-dependent subcutaneous ectopic bone formation within this system	549:617	Time- and dose-dependent subcutaneous ectopic bone formation within this system and bone regeneration capacity in a calvarial defect model were investigated.
29697853	9	56	theme	promising	1563:1571	arg1	results					1573:1579	promising results	1563:1579	promising results	1563:1579	Overall, this injectable BMP-2 delivery system showed promising results with 50 µg/mL BMP-2 in both the ectopic and calvarial rat defect models, underling the potential of this composite hydrogel for bone regeneration therapies.
29697853	0	57	theme	collagen-derived	42:57	arg1	microspheres					59:70	collagen-derived microspheres	42:70	collagen-derived microspheres	42:70	Injectable BMP-2 delivery system based on collagen-derived microspheres and alginate induced bone formation in a time- and dose-dependent manner.
29697853	5	58	theme	fluorescently-labelled	1060:1081	arg1	BMP-2					1083:1087	fluorescently-labelled BMP-2	1060:1087	fluorescently-labelled BMP-2	1060:1087	In addition, the spatio-temporal control of BMP-2 retention was shown for 4 weeks in vivo by imaging of fluorescently-labelled BMP-2.
29697853	4	59	theme	bone	821:824	arg1	formation					826:834	ectopic bone formation	813:834	ectopic bone formation	813:834	BMP-2 doses of 10 µg, 3 µg and 1 µg per implant (50 µg/mL, 15 µg/mL and 5 µg/mL, respectively) successfully induced ectopic bone formation subcutaneously in rats in a time- and dose-dependent manner, as shown by micro-computed tomography (µCT) and histology.
29697853	1	60	theme	release	363:369	arg1	system					371:376	a more effective release system	346:376	a more effective release system	346:376	The aim of the current study was to reduce the clinically used supra-physiological dose of bone morphogenetic protein-2 (BMP-2) (usually 1.5 mg/mL), which carries the risk of adverse events, by using a more effective release system.
29697853	3	61	theme	subcutaneous	564:575	arg1	formation					590:598	dose-dependent subcutaneous ectopic bone formation	549:598	dose-dependent subcutaneous ectopic bone formation within this system	549:617	Time- and dose-dependent subcutaneous ectopic bone formation within this system and bone regeneration capacity in a calvarial defect model were investigated.
29697853	4	62	theme	BMP-2	697:701	arg1	doses					703:707	BMP-2 doses	697:707	BMP-2 doses of 10 µg, 3 µg and 1 µg per implant (50 µg/mL, 15 µg/mL and 5 µg/mL, respectively)	697:790	BMP-2 doses of 10 µg, 3 µg and 1 µg per implant (50 µg/mL, 15 µg/mL and 5 µg/mL, respectively) successfully induced ectopic bone formation subcutaneously in rats in a time- and dose-dependent manner, as shown by micro-computed tomography (µCT) and histology.
29697853	3	63	from	capacity	641:648	arg1	model					672:676	a calvarial defect model	653:676	a calvarial defect model	653:676	Time- and dose-dependent subcutaneous ectopic bone formation within this system and bone regeneration capacity in a calvarial defect model were investigated.
29697853	5	64	theme	BMP-2	1083:1087	arg1	imaging					1049:1055	imaging	1049:1055	imaging of fluorescently-labelled BMP-2	1049:1087	In addition, the spatio-temporal control of BMP-2 retention was shown for 4 weeks in vivo by imaging of fluorescently-labelled BMP-2.
29697853	6	65	theme	implant	1193:1199	arg1	condition					1201:1209	implant condition	1193:1209	implant condition (50 µg/mL)	1193:1220	In the subcritical calvarial defect model, µCT revealed a higher bone volume for the 2 µg of BMP-2 per implant condition (50 µg/mL) as compared to the lower dose used (0.2 µg per implant, 5 µg/mL).
29697853	6	65	theme	implant	1193:1199	arg1	µg/mL					1215:1219	50 µg/mL	1212:1219	50 µg/mL	1212:1219	In the subcritical calvarial defect model, µCT revealed a higher bone volume for the 2 µg of BMP-2 per implant condition (50 µg/mL) as compared to the lower dose used (0.2 µg per implant, 5 µg/mL).
29697853	3	66	theme	defect	665:670	arg1	model					672:676	a calvarial defect model	653:676	a calvarial defect model	653:676	Time- and dose-dependent subcutaneous ectopic bone formation within this system and bone regeneration capacity in a calvarial defect model were investigated.
29697853	1	67	theme	supra-physiological	209:227	arg1	dose					229:232	the clinically used supra-physiological dose	189:232	the clinically used supra-physiological dose of bone morphogenetic protein-2 (BMP-2) (usually 1.5 mg/mL), which carries the risk of adverse events	189:334	The aim of the current study was to reduce the clinically used supra-physiological dose of bone morphogenetic protein-2 (BMP-2) (usually 1.5 mg/mL), which carries the risk of adverse events, by using a more effective release system.
29697853	9	68	from	BMP-2	1595:1599	arg1	calvarial					1625:1633	calvarial	1625:1633	calvarial	1625:1633	Overall, this injectable BMP-2 delivery system showed promising results with 50 µg/mL BMP-2 in both the ectopic and calvarial rat defect models, underling the potential of this composite hydrogel for bone regeneration therapies.
29697853	9	68	from	BMP-2	1595:1599	arg1	ectopic					1613:1619	ectopic	1613:1619	ectopic	1613:1619	Overall, this injectable BMP-2 delivery system showed promising results with 50 µg/mL BMP-2 in both the ectopic and calvarial rat defect models, underling the potential of this composite hydrogel for bone regeneration therapies.
29697853	9	69	theme	injectable	1523:1532	arg1	system					1549:1554	this injectable BMP-2 delivery system	1518:1554	this injectable BMP-2 delivery system	1518:1554	Overall, this injectable BMP-2 delivery system showed promising results with 50 µg/mL BMP-2 in both the ectopic and calvarial rat defect models, underling the potential of this composite hydrogel for bone regeneration therapies.
29697853	1	70	contain	carries	301:307	arg1	protein-2					256:264	bone morphogenetic protein-2	237:264	bone morphogenetic protein-2 (BMP-2) (usually 1.5 mg/mL)	237:292	The aim of the current study was to reduce the clinically used supra-physiological dose of bone morphogenetic protein-2 (BMP-2) (usually 1.5 mg/mL), which carries the risk of adverse events, by using a more effective release system.
29697853	1	70	contain	carries	301:307	arg1	BMP-2					267:271	BMP-2	267:271	BMP-2	267:271	The aim of the current study was to reduce the clinically used supra-physiological dose of bone morphogenetic protein-2 (BMP-2) (usually 1.5 mg/mL), which carries the risk of adverse events, by using a more effective release system.
29697853	1	70	contain	carries	301:307	arg1	mg/mL					287:291	usually 1.5 mg/mL	275:291	usually 1.5 mg/mL	275:291	The aim of the current study was to reduce the clinically used supra-physiological dose of bone morphogenetic protein-2 (BMP-2) (usually 1.5 mg/mL), which carries the risk of adverse events, by using a more effective release system.
29697853	1	70	contain	carries	301:307	arg2	risk					313:316	the risk	309:316	the risk of adverse events	309:334	The aim of the current study was to reduce the clinically used supra-physiological dose of bone morphogenetic protein-2 (BMP-2) (usually 1.5 mg/mL), which carries the risk of adverse events, by using a more effective release system.
29697853	3	71	from	Time-	539:543	arg1	model					672:676	a calvarial defect model	653:676	a calvarial defect model	653:676	Time- and dose-dependent subcutaneous ectopic bone formation within this system and bone regeneration capacity in a calvarial defect model were investigated.
29697853	9	72	theme	regeneration	1714:1725	arg1	therapies					1727:1735	bone regeneration therapies	1709:1735	bone regeneration therapies	1709:1735	Overall, this injectable BMP-2 delivery system showed promising results with 50 µg/mL BMP-2 in both the ectopic and calvarial rat defect models, underling the potential of this composite hydrogel for bone regeneration therapies.
29697853	2	73	theme	BMP-2-loaded	446:457	arg1	microspheres					486:497	BMP-2-loaded recombinant collagen-based microspheres	446:497	BMP-2-loaded recombinant collagen-based microspheres	446:497	A slow release system, based on an injectable hydrogel composed of BMP-2-loaded recombinant collagen-based microspheres and alginate, was previously developed.
29697853	5	74	theme	spatio-temporal	973:987	arg1	control					989:995	the spatio-temporal control	969:995	the spatio-temporal control of BMP-2 retention	969:1014	In addition, the spatio-temporal control of BMP-2 retention was shown for 4 weeks in vivo by imaging of fluorescently-labelled BMP-2.
29697853	6	75	theme	defect	1119:1124	arg1	model					1126:1130	the subcritical calvarial defect model	1093:1130	the subcritical calvarial defect model	1093:1130	In the subcritical calvarial defect model, µCT revealed a higher bone volume for the 2 µg of BMP-2 per implant condition (50 µg/mL) as compared to the lower dose used (0.2 µg per implant, 5 µg/mL).
29697853	9	76	theme	composite	1686:1694	arg1	hydrogel					1696:1703	this composite hydrogel	1681:1703	this composite hydrogel	1681:1703	Overall, this injectable BMP-2 delivery system showed promising results with 50 µg/mL BMP-2 in both the ectopic and calvarial rat defect models, underling the potential of this composite hydrogel for bone regeneration therapies.
29697853	8	77	theme	faster	1442:1447	arg1	defect					1435:1440	a calvarial defect	1423:1440	a calvarial defect faster than the empty defect or biomaterial control without BMP-2	1423:1506	The BMP-2 concentration of 5 µg/mL was not sufficient to heal a calvarial defect faster than the empty defect or biomaterial control without BMP-2.
29697853	9	78	theme	50	1586:1587	arg1	µg/mL					1589:1593	µg/mL	1589:1593	µg/mL	1589:1593	Overall, this injectable BMP-2 delivery system showed promising results with 50 µg/mL BMP-2 in both the ectopic and calvarial rat defect models, underling the potential of this composite hydrogel for bone regeneration therapies.
29697853	7	79	theme	50	1331:1332	arg1	µg/mL					1334:1338	µg/mL	1334:1338	µg/mL	1334:1338	Complete defect bridging was obtained with 50 µg/mL BMP-2 after 8 weeks.
29697853	0	80	theme	delivery	17:24	arg1	system					26:31	Injectable BMP-2 delivery system	0:31	Injectable BMP-2 delivery system based on collagen-derived microspheres and alginate	0:83	Injectable BMP-2 delivery system based on collagen-derived microspheres and alginate induced bone formation in a time- and dose-dependent manner.
29697853	8	81	theme	calvarial	1425:1433	arg1	defect					1435:1440	a calvarial defect	1423:1440	a calvarial defect faster than the empty defect or biomaterial control without BMP-2	1423:1506	The BMP-2 concentration of 5 µg/mL was not sufficient to heal a calvarial defect faster than the empty defect or biomaterial control without BMP-2.
29697853	9	82	theme	hydrogel	1696:1703	arg1	potential					1668:1676	the potential	1664:1676	the potential of this composite hydrogel for bone regeneration therapies	1664:1735	Overall, this injectable BMP-2 delivery system showed promising results with 50 µg/mL BMP-2 in both the ectopic and calvarial rat defect models, underling the potential of this composite hydrogel for bone regeneration therapies.
29697853	1	83	theme	bone	237:240	arg1	protein-2					256:264	bone morphogenetic protein-2	237:264	bone morphogenetic protein-2 (BMP-2) (usually 1.5 mg/mL)	237:292	The aim of the current study was to reduce the clinically used supra-physiological dose of bone morphogenetic protein-2 (BMP-2) (usually 1.5 mg/mL), which carries the risk of adverse events, by using a more effective release system.
29697853	1	83	theme	bone	237:240	arg1	mg/mL					287:291	usually 1.5 mg/mL	275:291	usually 1.5 mg/mL	275:291	The aim of the current study was to reduce the clinically used supra-physiological dose of bone morphogenetic protein-2 (BMP-2) (usually 1.5 mg/mL), which carries the risk of adverse events, by using a more effective release system.
29697853	1	83	theme	bone	237:240	arg1	BMP-2					267:271	BMP-2	267:271	BMP-2	267:271	The aim of the current study was to reduce the clinically used supra-physiological dose of bone morphogenetic protein-2 (BMP-2) (usually 1.5 mg/mL), which carries the risk of adverse events, by using a more effective release system.
31310200	8	0	theme	amino	1149:1153	arg1	sequences					1160:1168	corresponding amino acid sequences	1135:1168	corresponding amino acid sequences	1135:1168	Comparison of the partial housekeeping gene sequence of fusA showed higher sequence similarities of 812CHT to C. pelargi (95.8 %) than W8T (90.9 %) which was also confirmed by corresponding amino acid sequences.
31310200	8	1	theme	gene	998:1001	arg1	sequence					1003:1010	the partial housekeeping gene sequence	973:1010	the partial housekeeping gene sequence of fusA	973:1018	Comparison of the partial housekeeping gene sequence of fusA showed higher sequence similarities of 812CHT to C. pelargi (95.8 %) than W8T (90.9 %) which was also confirmed by corresponding amino acid sequences.
31310200	12	2	theme	novel	1671:1675	arg1	species					1677:1683	a novel species	1669:1683	a novel species	1669:1683	These data not only demonstrate that strain W8T is a representative of a novel species, but despite the high 16S rRNA gene sequence similarity to C. pelargi, strain 812CHT is also a representative of another novel species.
31310200	12	3	theme	high	1702:1705	arg1	similarity					1730:1739	the high 16S rRNA gene sequence similarity	1698:1739	the high 16S rRNA gene sequence similarity to C. pelargi	1698:1753	These data not only demonstrate that strain W8T is a representative of a novel species, but despite the high 16S rRNA gene sequence similarity to C. pelargi, strain 812CHT is also a representative of another novel species.
31310200	6	4	theme	rRNA	550:553	arg1	sequence					560:567	The 16S rRNA gene sequence	542:567	The 16S rRNA gene sequence of 200CHT	542:577	The 16S rRNA gene sequence of 200CHT showed highest similarities to Corynebacterium epidermidicanis 410T (96.7 %) followed by Corynebacterium argentoratense DSM 44202T, Corynebacterium ulcerans NCTC 7910T and Corynebacterium pseudotuberculosis CIP 102968T (each 96.3 %).
31310200	9	5	theme	fusA	1180:1183	arg1	gene					1185:1188	fusA gene and corresponding protein sequence strain 200CHT	1180:1237	gene	1185:1188	In both, fusA gene and corresponding protein sequence strain 200CHT showed low sequence similarities to C. epidermidicanis 410T(81.6 and 87.4 %, respectively).
31310200	7	6	theme	pelargi	911:917	arg1	136/3T					919:924	Corynebacterium pelargi 136/3T	895:924	Corynebacterium pelargi 136/3T (98.0 and 99.9 %, respectively)	895:956	Strains W8T and 812CHT both showed highest 16S rRNA gene sequence similarities to Corynebacterium pelargi 136/3T (98.0 and 99.9 %, respectively).
31310200	15	7	theme	30627T=CCM	2232:2241	arg1	8832T					2243:2247	=LMG 30627T=CCM 8832T	2227:2247	=LMG 30627T=CCM 8832T	2227:2247	In conclusion, three novel species of the genus Corynebacterium are proposed, namely Corynebacteriumpseudopelargi 812CHT (=LMG 30627T=CCM 8832T), Corynebacterium choanae 200CHT (=LMG 30628T=CCM 8831T) and Corynebacteriumgerontici W8T (=LMG 30629T=CCM 8833T), respectively.
31310200	15	7	theme	30627T=CCM	2232:2241	arg1	812CHT					2219:2224	Corynebacteriumpseudopelargi 812CHT	2190:2224	Corynebacteriumpseudopelargi 812CHT (=LMG 30627T=CCM 8832T)	2190:2248	In conclusion, three novel species of the genus Corynebacterium are proposed, namely Corynebacteriumpseudopelargi 812CHT (=LMG 30627T=CCM 8832T), Corynebacterium choanae 200CHT (=LMG 30628T=CCM 8831T) and Corynebacteriumgerontici W8T (=LMG 30629T=CCM 8833T), respectively.
31310200	1	8	theme	pseudopelargi	169:181	arg1	sp					183:184	Corynebacterium pseudopelargi sp	153:184	Corynebacterium pseudopelargi sp	153:184	nov., Corynebacterium pseudopelargi sp.
31310200	1	8	theme	pseudopelargi	169:181	arg1	nov.					147:150	nov.	147:150	nov.	147:150	nov., Corynebacterium pseudopelargi sp.
31310200	8	9	theme	sequence	1034:1041	arg1	similarities					1043:1054	higher sequence similarities	1027:1054	higher sequence similarities of 812CHT to C. pelargi (95.8 %)	1027:1087	Comparison of the partial housekeeping gene sequence of fusA showed higher sequence similarities of 812CHT to C. pelargi (95.8 %) than W8T (90.9 %) which was also confirmed by corresponding amino acid sequences.
31310200	9	10	theme	protein	1208:1214	arg1	200CHT					1232:1237	fusA gene and corresponding protein sequence strain 200CHT	1180:1237	200CHT	1232:1237	In both, fusA gene and corresponding protein sequence strain 200CHT showed low sequence similarities to C. epidermidicanis 410T(81.6 and 87.4 %, respectively).
31310200	15	11	theme	30628T=CCM	2288:2297	arg1	8831T					2299:2303	=LMG 30628T=CCM 8831T	2283:2303	=LMG 30628T=CCM 8831T	2283:2303	In conclusion, three novel species of the genus Corynebacterium are proposed, namely Corynebacteriumpseudopelargi 812CHT (=LMG 30627T=CCM 8832T), Corynebacterium choanae 200CHT (=LMG 30628T=CCM 8831T) and Corynebacteriumgerontici W8T (=LMG 30629T=CCM 8833T), respectively.
31310200	15	11	theme	30628T=CCM	2288:2297	arg1	200CHT					2275:2280	Corynebacterium choanae 200CHT	2251:2280	Corynebacterium choanae 200CHT (=LMG 30628T=CCM 8831T)	2251:2304	In conclusion, three novel species of the genus Corynebacterium are proposed, namely Corynebacteriumpseudopelargi 812CHT (=LMG 30627T=CCM 8832T), Corynebacterium choanae 200CHT (=LMG 30628T=CCM 8831T) and Corynebacteriumgerontici W8T (=LMG 30629T=CCM 8833T), respectively.
31310200	7	12	theme	sequence	870:877	arg1	similarities					879:890	highest 16S rRNA gene sequence similarities	848:890	highest 16S rRNA gene sequence similarities to Corynebacterium pelargi 136/3T (98.0 and 99.9 %, respectively)	848:956	Strains W8T and 812CHT both showed highest 16S rRNA gene sequence similarities to Corynebacterium pelargi 136/3T (98.0 and 99.9 %, respectively).
31310200	12	13	theme	species	1677:1683	arg1	W8T					1642:1644	strain W8T	1635:1644	strain W8T	1635:1644	These data not only demonstrate that strain W8T is a representative of a novel species, but despite the high 16S rRNA gene sequence similarity to C. pelargi, strain 812CHT is also a representative of another novel species.
31310200	12	13	theme	species	1677:1683	arg1	representative					1651:1664	a representative	1649:1664	a representative of a novel species	1649:1683	These data not only demonstrate that strain W8T is a representative of a novel species, but despite the high 16S rRNA gene sequence similarity to C. pelargi, strain 812CHT is also a representative of another novel species.
31310200	2	14	dep	sp	223:224	arg1	gerontici					213:221	Corynebacterium gerontici	197:221	Corynebacterium gerontici	197:221	nov., and Corynebacterium gerontici sp.
31310200	2	14	dep	sp	223:224	arg1	nov.					187:190	nov.	187:190	nov.	187:190	nov., and Corynebacterium gerontici sp.
31310200	9	15	theme	strain	1225:1230	arg1	200CHT					1232:1237	fusA gene and corresponding protein sequence strain 200CHT	1180:1237	200CHT	1232:1237	In both, fusA gene and corresponding protein sequence strain 200CHT showed low sequence similarities to C. epidermidicanis 410T(81.6 and 87.4 %, respectively).
31310200	7	16	theme	rRNA	860:863	arg1	similarities					879:890	highest 16S rRNA gene sequence similarities	848:890	highest 16S rRNA gene sequence similarities to Corynebacterium pelargi 136/3T (98.0 and 99.9 %, respectively)	848:956	Strains W8T and 812CHT both showed highest 16S rRNA gene sequence similarities to Corynebacterium pelargi 136/3T (98.0 and 99.9 %, respectively).
31310200	13	17	theme	contained	1873:1881	arg1	acid					1934:1937	the diagnostic diamino acid	1911:1937	the diagnostic diamino acid of the peptidoglycan	1911:1958	All three strains possessed corynemycolic acids and contained meso-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan.
31310200	13	17	theme	contained	1873:1881	arg1	acid					1903:1906	contained meso-diaminopimelic acid	1873:1906	contained meso-diaminopimelic acid	1873:1906	All three strains possessed corynemycolic acids and contained meso-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan.
31310200	13	17	theme	contained	1873:1881	arg1	acids					1863:1867	corynemycolic acids	1849:1867	corynemycolic acids	1849:1867	All three strains possessed corynemycolic acids and contained meso-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan.
31310200	6	18	dep	Corynebacterium	610:624	arg1	epidermidicanis					626:640	epidermidicanis	626:640	epidermidicanis	626:640	The 16S rRNA gene sequence of 200CHT showed highest similarities to Corynebacterium epidermidicanis 410T (96.7 %) followed by Corynebacterium argentoratense DSM 44202T, Corynebacterium ulcerans NCTC 7910T and Corynebacterium pseudotuberculosis CIP 102968T (each 96.3 %).
31310200	0	19	theme	Corynebacterium	119:133	arg1	sp					143:144	Corynebacterium choanae sp	119:144	Corynebacterium choanae sp	119:144	Classification of three corynebacterial strains isolated from the Northern Bald Ibis (Geronticus eremita): proposal of Corynebacterium choanae sp.
31310200	4	20	theme	comprehensive	459:471	arg1	characterization					483:498	comprehensive taxonomic characterization	459:498	comprehensive taxonomic characterization	459:498	Three Gram-stain-positive, rod-to-coccoid-shaped, catalase-positive and non-motile bacterial strains isolated from the choanae of a Northern bald ibis, designated strains 200CHT, W8T and 812CHT, respectively, were subjected to comprehensive taxonomic characterization.
31310200	6	21	theme	highest	586:592	arg1	similarities					594:605	highest similarities	586:605	highest similarities to Corynebacterium epidermidicanis 410T (96.7 %) followed by Corynebacterium argentoratense DSM 44202T, Corynebacterium ulcerans NCTC 7910T and Corynebacterium pseudotuberculosis CIP 102968T (each 96.3 %)	586:810	The 16S rRNA gene sequence of 200CHT showed highest similarities to Corynebacterium epidermidicanis 410T (96.7 %) followed by Corynebacterium argentoratense DSM 44202T, Corynebacterium ulcerans NCTC 7910T and Corynebacterium pseudotuberculosis CIP 102968T (each 96.3 %).
31310200	0	22	theme	sp	143:144	arg1	proposal					107:114	proposal	107:114	Classification of three corynebacterial strains isolated from the Northern Bald Ibis (Geronticus eremita): proposal of Corynebacterium choanae sp.	0:145	Classification of three corynebacterial strains isolated from the Northern Bald Ibis (Geronticus eremita): proposal of Corynebacterium choanae sp.
31310200	9	23	theme	sequence	1250:1257	arg1	similarities					1259:1270	low sequence similarities	1246:1270	low sequence similarities to C. epidermidicanis 410T(81.6 and 87.4 %, respectively)	1246:1328	In both, fusA gene and corresponding protein sequence strain 200CHT showed low sequence similarities to C. epidermidicanis 410T(81.6 and 87.4 %, respectively).
31310200	15	24	theme	genus	2147:2151	arg1	Corynebacterium					2153:2167	the genus Corynebacterium	2143:2167	the genus Corynebacterium	2143:2167	In conclusion, three novel species of the genus Corynebacterium are proposed, namely Corynebacteriumpseudopelargi 812CHT (=LMG 30627T=CCM 8832T), Corynebacterium choanae 200CHT (=LMG 30628T=CCM 8831T) and Corynebacteriumgerontici W8T (=LMG 30629T=CCM 8833T), respectively.
31310200	12	25	theme	strain	1635:1640	arg1	W8T					1642:1644	strain W8T	1635:1644	strain W8T	1635:1644	These data not only demonstrate that strain W8T is a representative of a novel species, but despite the high 16S rRNA gene sequence similarity to C. pelargi, strain 812CHT is also a representative of another novel species.
31310200	12	25	theme	strain	1635:1640	arg1	representative					1651:1664	a representative	1649:1664	a representative of a novel species	1649:1683	These data not only demonstrate that strain W8T is a representative of a novel species, but despite the high 16S rRNA gene sequence similarity to C. pelargi, strain 812CHT is also a representative of another novel species.
31310200	6	26	theme	DSM	699:701	arg1	%					809:809	each 96.3 %	799:809	each 96.3 %	799:809	The 16S rRNA gene sequence of 200CHT showed highest similarities to Corynebacterium epidermidicanis 410T (96.7 %) followed by Corynebacterium argentoratense DSM 44202T, Corynebacterium ulcerans NCTC 7910T and Corynebacterium pseudotuberculosis CIP 102968T (each 96.3 %).
31310200	6	26	theme	DSM	699:701	arg1	44202T					703:708	Corynebacterium argentoratense DSM 44202T	668:708	Corynebacterium argentoratense DSM 44202T	668:708	The 16S rRNA gene sequence of 200CHT showed highest similarities to Corynebacterium epidermidicanis 410T (96.7 %) followed by Corynebacterium argentoratense DSM 44202T, Corynebacterium ulcerans NCTC 7910T and Corynebacterium pseudotuberculosis CIP 102968T (each 96.3 %).
31310200	6	27	theme	Corynebacterium	668:682	arg1	%					809:809	each 96.3 %	799:809	each 96.3 %	799:809	The 16S rRNA gene sequence of 200CHT showed highest similarities to Corynebacterium epidermidicanis 410T (96.7 %) followed by Corynebacterium argentoratense DSM 44202T, Corynebacterium ulcerans NCTC 7910T and Corynebacterium pseudotuberculosis CIP 102968T (each 96.3 %).
31310200	6	27	theme	Corynebacterium	668:682	arg1	44202T					703:708	Corynebacterium argentoratense DSM 44202T	668:708	Corynebacterium argentoratense DSM 44202T	668:708	The 16S rRNA gene sequence of 200CHT showed highest similarities to Corynebacterium epidermidicanis 410T (96.7 %) followed by Corynebacterium argentoratense DSM 44202T, Corynebacterium ulcerans NCTC 7910T and Corynebacterium pseudotuberculosis CIP 102968T (each 96.3 %).
31310200	13	28	theme	diagnostic	1915:1924	arg1	acid					1934:1937	the diagnostic diamino acid	1911:1937	the diagnostic diamino acid of the peptidoglycan	1911:1958	All three strains possessed corynemycolic acids and contained meso-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan.
31310200	13	28	theme	diagnostic	1915:1924	arg1	acid					1903:1906	contained meso-diaminopimelic acid	1873:1906	contained meso-diaminopimelic acid	1873:1906	All three strains possessed corynemycolic acids and contained meso-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan.
31310200	13	28	theme	diagnostic	1915:1924	arg1	acids					1863:1867	corynemycolic acids	1849:1867	corynemycolic acids	1849:1867	All three strains possessed corynemycolic acids and contained meso-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan.
31310200	0	29	theme	Northern	66:73	arg1	eremita					97:103	the Northern Bald Ibis (Geronticus eremita)	62:104	the Northern Bald Ibis (Geronticus eremita)	62:104	Classification of three corynebacterial strains isolated from the Northern Bald Ibis (Geronticus eremita): proposal of Corynebacterium choanae sp.
31310200	9	30	theme	epidermidicanis	1278:1292	arg1	410T					1294:1297	C. epidermidicanis 410T	1275:1297	C. epidermidicanis 410T(81.6 and 87.4 %, respectively)	1275:1328	In both, fusA gene and corresponding protein sequence strain 200CHT showed low sequence similarities to C. epidermidicanis 410T(81.6 and 87.4 %, respectively).
31310200	15	31	theme	novel	2126:2130	arg1	species					2132:2138	three novel species	2120:2138	three novel species of the genus Corynebacterium	2120:2167	In conclusion, three novel species of the genus Corynebacterium are proposed, namely Corynebacteriumpseudopelargi 812CHT (=LMG 30627T=CCM 8832T), Corynebacterium choanae 200CHT (=LMG 30628T=CCM 8831T) and Corynebacteriumgerontici W8T (=LMG 30629T=CCM 8833T), respectively.
31310200	0	32	theme	Ibis	80:83	arg1	eremita					97:103	the Northern Bald Ibis (Geronticus eremita)	62:104	the Northern Bald Ibis (Geronticus eremita)	62:104	Classification of three corynebacterial strains isolated from the Northern Bald Ibis (Geronticus eremita): proposal of Corynebacterium choanae sp.
31310200	12	33	theme	novel	1806:1810	arg1	species					1812:1818	another novel species	1798:1818	another novel species	1798:1818	These data not only demonstrate that strain W8T is a representative of a novel species, but despite the high 16S rRNA gene sequence similarity to C. pelargi, strain 812CHT is also a representative of another novel species.
31310200	8	34	dep	812CHT	1059:1064	arg1	pelargi					1072:1078	pelargi	1072:1078	pelargi	1072:1078	Comparison of the partial housekeeping gene sequence of fusA showed higher sequence similarities of 812CHT to C. pelargi (95.8 %) than W8T (90.9 %) which was also confirmed by corresponding amino acid sequences.
31310200	8	34	dep	812CHT	1059:1064	arg1	to					1066:1067	to	1066:1067	to	1066:1067	Comparison of the partial housekeeping gene sequence of fusA showed higher sequence similarities of 812CHT to C. pelargi (95.8 %) than W8T (90.9 %) which was also confirmed by corresponding amino acid sequences.
31310200	10	35	theme	%	1363:1363	arg1	similarity					1369:1378	76.7 % ANI similarity	1358:1378	76.7 % ANI similarity to each other	1358:1392	Strains 812CHT and W8T had 76.7 % ANI similarity to each other and 88.2 and 76.4 % to C. pelargi 136/3T, respectively.
31310200	11	36	theme	DNA-DNA	1460:1466	arg1	hybridization					1468:1480	In silico DNA-DNA hybridization	1450:1480	In silico DNA-DNA hybridization values for 812CHT and W8T	1450:1506	In silico DNA-DNA hybridization values for 812CHT and W8T were 22.1 % among the two strains and 35.3 and 21.7 % to C. pelargi 136/3T, respectively.
31310200	0	37	theme	Geronticus	86:95	arg1	eremita					97:103	the Northern Bald Ibis (Geronticus eremita)	62:104	the Northern Bald Ibis (Geronticus eremita)	62:104	Classification of three corynebacterial strains isolated from the Northern Bald Ibis (Geronticus eremita): proposal of Corynebacterium choanae sp.
31310200	13	38	theme	peptidoglycan	1946:1958	arg1	acid					1934:1937	the diagnostic diamino acid	1911:1937	the diagnostic diamino acid of the peptidoglycan	1911:1958	All three strains possessed corynemycolic acids and contained meso-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan.
31310200	13	38	theme	peptidoglycan	1946:1958	arg1	acid					1903:1906	contained meso-diaminopimelic acid	1873:1906	contained meso-diaminopimelic acid	1873:1906	All three strains possessed corynemycolic acids and contained meso-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan.
31310200	13	38	theme	peptidoglycan	1946:1958	arg1	acids					1863:1867	corynemycolic acids	1849:1867	corynemycolic acids	1849:1867	All three strains possessed corynemycolic acids and contained meso-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan.
31310200	4	39	theme	bacterial	315:323	arg1	strains					325:331	Three Gram-stain-positive, rod-to-coccoid-shaped, catalase-positive and non-motile bacterial strains	232:331	Three Gram-stain-positive, rod-to-coccoid-shaped, catalase-positive and non-motile bacterial strains isolated from the choanae of a Northern bald ibis	232:381	Three Gram-stain-positive, rod-to-coccoid-shaped, catalase-positive and non-motile bacterial strains isolated from the choanae of a Northern bald ibis, designated strains 200CHT, W8T and 812CHT, respectively, were subjected to comprehensive taxonomic characterization.
31310200	11	40	dep	In	1450:1451	arg1	silico					1453:1458	silico	1453:1458	silico	1453:1458	In silico DNA-DNA hybridization values for 812CHT and W8T were 22.1 % among the two strains and 35.3 and 21.7 % to C. pelargi 136/3T, respectively.
31310200	6	41	theme	Corynebacterium	751:765	arg1	102968T					790:796	Corynebacterium pseudotuberculosis CIP 102968T	751:796	Corynebacterium pseudotuberculosis CIP 102968T	751:796	The 16S rRNA gene sequence of 200CHT showed highest similarities to Corynebacterium epidermidicanis 410T (96.7 %) followed by Corynebacterium argentoratense DSM 44202T, Corynebacterium ulcerans NCTC 7910T and Corynebacterium pseudotuberculosis CIP 102968T (each 96.3 %).
31310200	10	42	theme	C.	1417:1418	arg1	136/3T					1428:1433	C. pelargi 136/3T	1417:1433	C. pelargi 136/3T	1417:1433	Strains 812CHT and W8T had 76.7 % ANI similarity to each other and 88.2 and 76.4 % to C. pelargi 136/3T, respectively.
31310200	7	43	theme	highest	848:854	arg1	similarities					879:890	highest 16S rRNA gene sequence similarities	848:890	highest 16S rRNA gene sequence similarities to Corynebacterium pelargi 136/3T (98.0 and 99.9 %, respectively)	848:956	Strains W8T and 812CHT both showed highest 16S rRNA gene sequence similarities to Corynebacterium pelargi 136/3T (98.0 and 99.9 %, respectively).
31310200	15	44	theme	=LMG	2283:2286	arg1	8831T					2299:2303	=LMG 30628T=CCM 8831T	2283:2303	=LMG 30628T=CCM 8831T	2283:2303	In conclusion, three novel species of the genus Corynebacterium are proposed, namely Corynebacteriumpseudopelargi 812CHT (=LMG 30627T=CCM 8832T), Corynebacterium choanae 200CHT (=LMG 30628T=CCM 8831T) and Corynebacteriumgerontici W8T (=LMG 30629T=CCM 8833T), respectively.
31310200	15	44	theme	=LMG	2283:2286	arg1	200CHT					2275:2280	Corynebacterium choanae 200CHT	2251:2280	Corynebacterium choanae 200CHT (=LMG 30628T=CCM 8831T)	2251:2304	In conclusion, three novel species of the genus Corynebacterium are proposed, namely Corynebacteriumpseudopelargi 812CHT (=LMG 30627T=CCM 8832T), Corynebacterium choanae 200CHT (=LMG 30628T=CCM 8831T) and Corynebacteriumgerontici W8T (=LMG 30629T=CCM 8833T), respectively.
31310200	12	45	dep	data	1604:1607	arg1	only					1613:1616	only	1613:1616	only	1613:1616	These data not only demonstrate that strain W8T is a representative of a novel species, but despite the high 16S rRNA gene sequence similarity to C. pelargi, strain 812CHT is also a representative of another novel species.
31310200	6	46	theme	ulcerans	727:734	arg1	7910T					741:745	Corynebacterium ulcerans NCTC 7910T	711:745	Corynebacterium ulcerans NCTC 7910T	711:745	The 16S rRNA gene sequence of 200CHT showed highest similarities to Corynebacterium epidermidicanis 410T (96.7 %) followed by Corynebacterium argentoratense DSM 44202T, Corynebacterium ulcerans NCTC 7910T and Corynebacterium pseudotuberculosis CIP 102968T (each 96.3 %).
31310200	12	47	theme	strain	1756:1761	arg1	812CHT					1763:1768	strain 812CHT	1756:1768	strain 812CHT	1756:1768	These data not only demonstrate that strain W8T is a representative of a novel species, but despite the high 16S rRNA gene sequence similarity to C. pelargi, strain 812CHT is also a representative of another novel species.
31310200	12	47	theme	strain	1756:1761	arg1	representative					1780:1793	a representative	1778:1793	a representative of another novel species	1778:1818	These data not only demonstrate that strain W8T is a representative of a novel species, but despite the high 16S rRNA gene sequence similarity to C. pelargi, strain 812CHT is also a representative of another novel species.
31310200	9	48	dep	410T	1294:1297	arg1	%					1313:1313	81.6 and 87.4 %	1299:1313	%	1313:1313	In both, fusA gene and corresponding protein sequence strain 200CHT showed low sequence similarities to C. epidermidicanis 410T(81.6 and 87.4 %, respectively).
31310200	0	49	theme	strains	40:46	arg1	Classification					0:13	Classification	0:13	Classification of three corynebacterial strains isolated from the Northern Bald Ibis (Geronticus eremita): proposal of Corynebacterium choanae sp.	0:145	Classification of three corynebacterial strains isolated from the Northern Bald Ibis (Geronticus eremita): proposal of Corynebacterium choanae sp.
31310200	4	50	theme	Northern	364:371	arg1	ibis					378:381	a Northern bald ibis	362:381	a Northern bald ibis	362:381	Three Gram-stain-positive, rod-to-coccoid-shaped, catalase-positive and non-motile bacterial strains isolated from the choanae of a Northern bald ibis, designated strains 200CHT, W8T and 812CHT, respectively, were subjected to comprehensive taxonomic characterization.
31310200	13	51	contain	possessed	1839:1847	arg2	acid					1903:1906	contained meso-diaminopimelic acid	1873:1906	contained meso-diaminopimelic acid	1873:1906	All three strains possessed corynemycolic acids and contained meso-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan.
31310200	13	51	contain	possessed	1839:1847	arg2	acid					1934:1937	the diagnostic diamino acid	1911:1937	the diagnostic diamino acid of the peptidoglycan	1911:1958	All three strains possessed corynemycolic acids and contained meso-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan.
31310200	13	51	contain	possessed	1839:1847	arg1	strains					1831:1837	All three strains	1821:1837	All three strains	1821:1837	All three strains possessed corynemycolic acids and contained meso-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan.
31310200	13	51	contain	possessed	1839:1847	arg2	acids					1863:1867	corynemycolic acids	1849:1867	corynemycolic acids	1849:1867	All three strains possessed corynemycolic acids and contained meso-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan.
31310200	12	52	theme	sequence	1721:1728	arg1	similarity					1730:1739	the high 16S rRNA gene sequence similarity	1698:1739	the high 16S rRNA gene sequence similarity to C. pelargi	1698:1753	These data not only demonstrate that strain W8T is a representative of a novel species, but despite the high 16S rRNA gene sequence similarity to C. pelargi, strain 812CHT is also a representative of another novel species.
31310200	15	53	theme	Corynebacterium	2153:2167	arg1	species					2132:2138	three novel species	2120:2138	three novel species of the genus Corynebacterium	2120:2167	In conclusion, three novel species of the genus Corynebacterium are proposed, namely Corynebacteriumpseudopelargi 812CHT (=LMG 30627T=CCM 8832T), Corynebacterium choanae 200CHT (=LMG 30628T=CCM 8831T) and Corynebacteriumgerontici W8T (=LMG 30629T=CCM 8833T), respectively.
31310200	4	54	theme	ibis	378:381	arg1	choanae					351:357	the choanae	347:357	the choanae of a Northern bald ibis	347:381	Three Gram-stain-positive, rod-to-coccoid-shaped, catalase-positive and non-motile bacterial strains isolated from the choanae of a Northern bald ibis, designated strains 200CHT, W8T and 812CHT, respectively, were subjected to comprehensive taxonomic characterization.
31310200	7	55	dep	136/3T	919:924	arg1	%					941:941	98.0 and 99.9 %	927:941	%	941:941	Strains W8T and 812CHT both showed highest 16S rRNA gene sequence similarities to Corynebacterium pelargi 136/3T (98.0 and 99.9 %, respectively).
31310200	12	56	theme	gene	1716:1719	arg1	similarity					1730:1739	the high 16S rRNA gene sequence similarity	1698:1739	the high 16S rRNA gene sequence similarity to C. pelargi	1698:1753	These data not only demonstrate that strain W8T is a representative of a novel species, but despite the high 16S rRNA gene sequence similarity to C. pelargi, strain 812CHT is also a representative of another novel species.
31310200	8	57	theme	housekeeping	985:996	arg1	sequence					1003:1010	the partial housekeeping gene sequence	973:1010	the partial housekeeping gene sequence of fusA	973:1018	Comparison of the partial housekeeping gene sequence of fusA showed higher sequence similarities of 812CHT to C. pelargi (95.8 %) than W8T (90.9 %) which was also confirmed by corresponding amino acid sequences.
31310200	0	58	dep	Classification	0:13	arg1	proposal					107:114	proposal	107:114	Classification of three corynebacterial strains isolated from the Northern Bald Ibis (Geronticus eremita): proposal of Corynebacterium choanae sp.	0:145	Classification of three corynebacterial strains isolated from the Northern Bald Ibis (Geronticus eremita): proposal of Corynebacterium choanae sp.
31310200	8	59	theme	sequence	1003:1010	arg1	Comparison					959:968	Comparison	959:968	Comparison of the partial housekeeping gene sequence of fusA	959:1018	Comparison of the partial housekeeping gene sequence of fusA showed higher sequence similarities of 812CHT to C. pelargi (95.8 %) than W8T (90.9 %) which was also confirmed by corresponding amino acid sequences.
31310200	6	60	theme	CIP	786:788	arg1	102968T					790:796	Corynebacterium pseudotuberculosis CIP 102968T	751:796	Corynebacterium pseudotuberculosis CIP 102968T	751:796	The 16S rRNA gene sequence of 200CHT showed highest similarities to Corynebacterium epidermidicanis 410T (96.7 %) followed by Corynebacterium argentoratense DSM 44202T, Corynebacterium ulcerans NCTC 7910T and Corynebacterium pseudotuberculosis CIP 102968T (each 96.3 %).
31310200	7	61	theme	99.9 	936:940	arg1	%					941:941	98.0 and 99.9 %	927:941	%	941:941	Strains W8T and 812CHT both showed highest 16S rRNA gene sequence similarities to Corynebacterium pelargi 136/3T (98.0 and 99.9 %, respectively).
31310200	8	62	theme	corresponding	1135:1147	arg1	sequences					1160:1168	corresponding amino acid sequences	1135:1168	corresponding amino acid sequences	1135:1168	Comparison of the partial housekeeping gene sequence of fusA showed higher sequence similarities of 812CHT to C. pelargi (95.8 %) than W8T (90.9 %) which was also confirmed by corresponding amino acid sequences.
31310200	8	63	theme	fusA	1015:1018	arg1	sequence					1003:1010	the partial housekeeping gene sequence	973:1010	the partial housekeeping gene sequence of fusA	973:1018	Comparison of the partial housekeeping gene sequence of fusA showed higher sequence similarities of 812CHT to C. pelargi (95.8 %) than W8T (90.9 %) which was also confirmed by corresponding amino acid sequences.
31310200	6	64	theme	gene	555:558	arg1	sequence					560:567	The 16S rRNA gene sequence	542:567	The 16S rRNA gene sequence of 200CHT	542:577	The 16S rRNA gene sequence of 200CHT showed highest similarities to Corynebacterium epidermidicanis 410T (96.7 %) followed by Corynebacterium argentoratense DSM 44202T, Corynebacterium ulcerans NCTC 7910T and Corynebacterium pseudotuberculosis CIP 102968T (each 96.3 %).
31310200	0	65	attach	isolated	48:55	arg1	eremita					97:103	the Northern Bald Ibis (Geronticus eremita)	62:104	the Northern Bald Ibis (Geronticus eremita)	62:104	Classification of three corynebacterial strains isolated from the Northern Bald Ibis (Geronticus eremita): proposal of Corynebacterium choanae sp.
31310200	0	65	attach	isolated	48:55	arg2	strains					40:46	three corynebacterial strains	18:46	three corynebacterial strains isolated from the Northern Bald Ibis (Geronticus eremita)	18:104	Classification of three corynebacterial strains isolated from the Northern Bald Ibis (Geronticus eremita): proposal of Corynebacterium choanae sp.
31310200	10	66	theme	ANI	1365:1367	arg1	similarity					1369:1378	76.7 % ANI similarity	1358:1378	76.7 % ANI similarity to each other	1358:1392	Strains 812CHT and W8T had 76.7 % ANI similarity to each other and 88.2 and 76.4 % to C. pelargi 136/3T, respectively.
31310200	7	67	theme	98.0	927:930	arg1	%					941:941	98.0 and 99.9 %	927:941	%	941:941	Strains W8T and 812CHT both showed highest 16S rRNA gene sequence similarities to Corynebacterium pelargi 136/3T (98.0 and 99.9 %, respectively).
31310200	15	68	theme	=LMG	2227:2230	arg1	8832T					2243:2247	=LMG 30627T=CCM 8832T	2227:2247	=LMG 30627T=CCM 8832T	2227:2247	In conclusion, three novel species of the genus Corynebacterium are proposed, namely Corynebacteriumpseudopelargi 812CHT (=LMG 30627T=CCM 8832T), Corynebacterium choanae 200CHT (=LMG 30628T=CCM 8831T) and Corynebacteriumgerontici W8T (=LMG 30629T=CCM 8833T), respectively.
31310200	15	68	theme	=LMG	2227:2230	arg1	812CHT					2219:2224	Corynebacteriumpseudopelargi 812CHT	2190:2224	Corynebacteriumpseudopelargi 812CHT (=LMG 30627T=CCM 8832T)	2190:2248	In conclusion, three novel species of the genus Corynebacterium are proposed, namely Corynebacteriumpseudopelargi 812CHT (=LMG 30627T=CCM 8832T), Corynebacterium choanae 200CHT (=LMG 30628T=CCM 8831T) and Corynebacteriumgerontici W8T (=LMG 30629T=CCM 8833T), respectively.
31310200	8	69	theme	acid	1155:1158	arg1	sequences					1160:1168	corresponding amino acid sequences	1135:1168	corresponding amino acid sequences	1135:1168	Comparison of the partial housekeeping gene sequence of fusA showed higher sequence similarities of 812CHT to C. pelargi (95.8 %) than W8T (90.9 %) which was also confirmed by corresponding amino acid sequences.
31310200	7	70	dep	Strains	813:819	arg1	Strains					813:819	Strains	813:819	Strains W8T and 812CHT	813:834	Strains W8T and 812CHT both showed highest 16S rRNA gene sequence similarities to Corynebacterium pelargi 136/3T (98.0 and 99.9 %, respectively).
31310200	7	70	dep	Strains	813:819	arg1	812CHT					829:834	812CHT	829:834	812CHT	829:834	Strains W8T and 812CHT both showed highest 16S rRNA gene sequence similarities to Corynebacterium pelargi 136/3T (98.0 and 99.9 %, respectively).
31310200	7	70	dep	Strains	813:819	arg1	W8T					821:823	W8T	821:823	W8T	821:823	Strains W8T and 812CHT both showed highest 16S rRNA gene sequence similarities to Corynebacterium pelargi 136/3T (98.0 and 99.9 %, respectively).
31310200	8	71	theme	higher	1027:1032	arg1	similarities					1043:1054	higher sequence similarities	1027:1054	higher sequence similarities of 812CHT to C. pelargi (95.8 %)	1027:1087	Comparison of the partial housekeeping gene sequence of fusA showed higher sequence similarities of 812CHT to C. pelargi (95.8 %) than W8T (90.9 %) which was also confirmed by corresponding amino acid sequences.
31310200	6	72	theme	16S	546:548	arg1	rRNA					550:553	The 16S rRNA	542:553	The 16S rRNA gene sequence of 200CHT	542:577	The 16S rRNA gene sequence of 200CHT showed highest similarities to Corynebacterium epidermidicanis 410T (96.7 %) followed by Corynebacterium argentoratense DSM 44202T, Corynebacterium ulcerans NCTC 7910T and Corynebacterium pseudotuberculosis CIP 102968T (each 96.3 %).
31310200	10	73	theme	76.7 	1358:1362	arg1	%					1363:1363	%	1363:1363	%	1363:1363	Strains 812CHT and W8T had 76.7 % ANI similarity to each other and 88.2 and 76.4 % to C. pelargi 136/3T, respectively.
31310200	1	74	theme	Corynebacterium	153:167	arg1	sp					183:184	Corynebacterium pseudopelargi sp	153:184	Corynebacterium pseudopelargi sp	153:184	nov., Corynebacterium pseudopelargi sp.
31310200	1	74	theme	Corynebacterium	153:167	arg1	nov.					147:150	nov.	147:150	nov.	147:150	nov., Corynebacterium pseudopelargi sp.
31310200	4	75	theme	designated	384:393	arg1	strains					395:401	designated strains	384:401	designated strains 200CHT	384:408	Three Gram-stain-positive, rod-to-coccoid-shaped, catalase-positive and non-motile bacterial strains isolated from the choanae of a Northern bald ibis, designated strains 200CHT, W8T and 812CHT, respectively, were subjected to comprehensive taxonomic characterization.
31310200	9	76	theme	corresponding	1194:1206	arg1	200CHT					1232:1237	fusA gene and corresponding protein sequence strain 200CHT	1180:1237	200CHT	1232:1237	In both, fusA gene and corresponding protein sequence strain 200CHT showed low sequence similarities to C. epidermidicanis 410T(81.6 and 87.4 %, respectively).
31310200	7	77	theme	Corynebacterium	895:909	arg1	136/3T					919:924	Corynebacterium pelargi 136/3T	895:924	Corynebacterium pelargi 136/3T (98.0 and 99.9 %, respectively)	895:956	Strains W8T and 812CHT both showed highest 16S rRNA gene sequence similarities to Corynebacterium pelargi 136/3T (98.0 and 99.9 %, respectively).
31310200	8	78	theme	812CHT	1059:1064	arg1	similarities					1043:1054	higher sequence similarities	1027:1054	higher sequence similarities of 812CHT to C. pelargi (95.8 %)	1027:1087	Comparison of the partial housekeeping gene sequence of fusA showed higher sequence similarities of 812CHT to C. pelargi (95.8 %) than W8T (90.9 %) which was also confirmed by corresponding amino acid sequences.
31310200	9	79	theme	sequence	1216:1223	arg1	200CHT					1232:1237	fusA gene and corresponding protein sequence strain 200CHT	1180:1237	200CHT	1232:1237	In both, fusA gene and corresponding protein sequence strain 200CHT showed low sequence similarities to C. epidermidicanis 410T(81.6 and 87.4 %, respectively).
31310200	6	80	theme	Corynebacterium	610:624	arg1	410T					642:645	Corynebacterium epidermidicanis 410T	610:645	Corynebacterium epidermidicanis 410T (96.7 %) followed by Corynebacterium argentoratense DSM 44202T, Corynebacterium ulcerans NCTC 7910T and Corynebacterium pseudotuberculosis CIP 102968T (each 96.3 %)	610:810	The 16S rRNA gene sequence of 200CHT showed highest similarities to Corynebacterium epidermidicanis 410T (96.7 %) followed by Corynebacterium argentoratense DSM 44202T, Corynebacterium ulcerans NCTC 7910T and Corynebacterium pseudotuberculosis CIP 102968T (each 96.3 %).
31310200	6	80	theme	Corynebacterium	610:624	arg1	%					653:653	96.7 %	648:653	96.7 %	648:653	The 16S rRNA gene sequence of 200CHT showed highest similarities to Corynebacterium epidermidicanis 410T (96.7 %) followed by Corynebacterium argentoratense DSM 44202T, Corynebacterium ulcerans NCTC 7910T and Corynebacterium pseudotuberculosis CIP 102968T (each 96.3 %).
31310200	0	81	theme	choanae	135:141	arg1	sp					143:144	Corynebacterium choanae sp	119:144	Corynebacterium choanae sp	119:144	Classification of three corynebacterial strains isolated from the Northern Bald Ibis (Geronticus eremita): proposal of Corynebacterium choanae sp.
31310200	7	82	theme	gene	865:868	arg1	similarities					879:890	highest 16S rRNA gene sequence similarities	848:890	highest 16S rRNA gene sequence similarities to Corynebacterium pelargi 136/3T (98.0 and 99.9 %, respectively)	848:956	Strains W8T and 812CHT both showed highest 16S rRNA gene sequence similarities to Corynebacterium pelargi 136/3T (98.0 and 99.9 %, respectively).
31310200	4	83	attach	isolated	333:340	arg1	choanae					351:357	the choanae	347:357	the choanae of a Northern bald ibis	347:381	Three Gram-stain-positive, rod-to-coccoid-shaped, catalase-positive and non-motile bacterial strains isolated from the choanae of a Northern bald ibis, designated strains 200CHT, W8T and 812CHT, respectively, were subjected to comprehensive taxonomic characterization.
31310200	4	83	attach	isolated	333:340	arg2	strains					325:331	Three Gram-stain-positive, rod-to-coccoid-shaped, catalase-positive and non-motile bacterial strains	232:331	Three Gram-stain-positive, rod-to-coccoid-shaped, catalase-positive and non-motile bacterial strains isolated from the choanae of a Northern bald ibis	232:381	Three Gram-stain-positive, rod-to-coccoid-shaped, catalase-positive and non-motile bacterial strains isolated from the choanae of a Northern bald ibis, designated strains 200CHT, W8T and 812CHT, respectively, were subjected to comprehensive taxonomic characterization.
31310200	4	84	theme	taxonomic	473:481	arg1	characterization					483:498	comprehensive taxonomic characterization	459:498	comprehensive taxonomic characterization	459:498	Three Gram-stain-positive, rod-to-coccoid-shaped, catalase-positive and non-motile bacterial strains isolated from the choanae of a Northern bald ibis, designated strains 200CHT, W8T and 812CHT, respectively, were subjected to comprehensive taxonomic characterization.
31310200	14	85	theme	Corynebacterium	2044:2058	arg1	species					2060:2066	Corynebacterium species	2044:2066	Corynebacterium species	2044:2066	The two strains, 200CHT and W8T, are distinguished from each other and established Corynebacterium species phylogenetically and phenotypically.
31310200	9	86	theme	low	1246:1248	arg1	similarities					1259:1270	low sequence similarities	1246:1270	low sequence similarities to C. epidermidicanis 410T(81.6 and 87.4 %, respectively)	1246:1328	In both, fusA gene and corresponding protein sequence strain 200CHT showed low sequence similarities to C. epidermidicanis 410T(81.6 and 87.4 %, respectively).
31310200	13	87	theme	corynemycolic	1849:1861	arg1	acid					1934:1937	the diagnostic diamino acid	1911:1937	the diagnostic diamino acid of the peptidoglycan	1911:1958	All three strains possessed corynemycolic acids and contained meso-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan.
31310200	13	87	theme	corynemycolic	1849:1861	arg1	acid					1903:1906	contained meso-diaminopimelic acid	1873:1906	contained meso-diaminopimelic acid	1873:1906	All three strains possessed corynemycolic acids and contained meso-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan.
31310200	13	87	theme	corynemycolic	1849:1861	arg1	acids					1863:1867	corynemycolic acids	1849:1867	corynemycolic acids	1849:1867	All three strains possessed corynemycolic acids and contained meso-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan.
31310200	6	88	theme	96.3 	804:808	arg1	%					809:809	each 96.3 %	799:809	each 96.3 %	799:809	The 16S rRNA gene sequence of 200CHT showed highest similarities to Corynebacterium epidermidicanis 410T (96.7 %) followed by Corynebacterium argentoratense DSM 44202T, Corynebacterium ulcerans NCTC 7910T and Corynebacterium pseudotuberculosis CIP 102968T (each 96.3 %).
31310200	6	88	theme	96.3 	804:808	arg1	44202T					703:708	Corynebacterium argentoratense DSM 44202T	668:708	Corynebacterium argentoratense DSM 44202T	668:708	The 16S rRNA gene sequence of 200CHT showed highest similarities to Corynebacterium epidermidicanis 410T (96.7 %) followed by Corynebacterium argentoratense DSM 44202T, Corynebacterium ulcerans NCTC 7910T and Corynebacterium pseudotuberculosis CIP 102968T (each 96.3 %).
31310200	6	89	theme	argentoratense	684:697	arg1	%					809:809	each 96.3 %	799:809	each 96.3 %	799:809	The 16S rRNA gene sequence of 200CHT showed highest similarities to Corynebacterium epidermidicanis 410T (96.7 %) followed by Corynebacterium argentoratense DSM 44202T, Corynebacterium ulcerans NCTC 7910T and Corynebacterium pseudotuberculosis CIP 102968T (each 96.3 %).
31310200	6	89	theme	argentoratense	684:697	arg1	44202T					703:708	Corynebacterium argentoratense DSM 44202T	668:708	Corynebacterium argentoratense DSM 44202T	668:708	The 16S rRNA gene sequence of 200CHT showed highest similarities to Corynebacterium epidermidicanis 410T (96.7 %) followed by Corynebacterium argentoratense DSM 44202T, Corynebacterium ulcerans NCTC 7910T and Corynebacterium pseudotuberculosis CIP 102968T (each 96.3 %).
31310200	0	90	theme	Bald	75:78	arg1	eremita					97:103	the Northern Bald Ibis (Geronticus eremita)	62:104	the Northern Bald Ibis (Geronticus eremita)	62:104	Classification of three corynebacterial strains isolated from the Northern Bald Ibis (Geronticus eremita): proposal of Corynebacterium choanae sp.
31310200	9	91	theme	C.	1275:1276	arg1	410T					1294:1297	C. epidermidicanis 410T	1275:1297	C. epidermidicanis 410T(81.6 and 87.4 %, respectively)	1275:1328	In both, fusA gene and corresponding protein sequence strain 200CHT showed low sequence similarities to C. epidermidicanis 410T(81.6 and 87.4 %, respectively).
31310200	13	92	theme	meso-diaminopimelic	1883:1901	arg1	acid					1934:1937	the diagnostic diamino acid	1911:1937	the diagnostic diamino acid of the peptidoglycan	1911:1958	All three strains possessed corynemycolic acids and contained meso-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan.
31310200	13	92	theme	meso-diaminopimelic	1883:1901	arg1	acid					1903:1906	contained meso-diaminopimelic acid	1873:1906	contained meso-diaminopimelic acid	1873:1906	All three strains possessed corynemycolic acids and contained meso-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan.
31310200	13	92	theme	meso-diaminopimelic	1883:1901	arg1	acids					1863:1867	corynemycolic acids	1849:1867	corynemycolic acids	1849:1867	All three strains possessed corynemycolic acids and contained meso-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan.
31310200	4	93	theme	Gram-stain-positive	238:256	arg1	strains					325:331	Three Gram-stain-positive, rod-to-coccoid-shaped, catalase-positive and non-motile bacterial strains	232:331	Three Gram-stain-positive, rod-to-coccoid-shaped, catalase-positive and non-motile bacterial strains isolated from the choanae of a Northern bald ibis	232:381	Three Gram-stain-positive, rod-to-coccoid-shaped, catalase-positive and non-motile bacterial strains isolated from the choanae of a Northern bald ibis, designated strains 200CHT, W8T and 812CHT, respectively, were subjected to comprehensive taxonomic characterization.
31310200	11	94	theme	hybridization	1468:1480	arg1	values					1482:1487	In silico DNA-DNA hybridization values	1450:1487	In silico DNA-DNA hybridization values for 812CHT and W8T	1450:1506	In silico DNA-DNA hybridization values for 812CHT and W8T were 22.1 % among the two strains and 35.3 and 21.7 % to C. pelargi 136/3T, respectively.
31310200	12	95	theme	species	1812:1818	arg1	812CHT					1763:1768	strain 812CHT	1756:1768	strain 812CHT	1756:1768	These data not only demonstrate that strain W8T is a representative of a novel species, but despite the high 16S rRNA gene sequence similarity to C. pelargi, strain 812CHT is also a representative of another novel species.
31310200	12	95	theme	species	1812:1818	arg1	representative					1780:1793	a representative	1778:1793	a representative of another novel species	1778:1818	These data not only demonstrate that strain W8T is a representative of a novel species, but despite the high 16S rRNA gene sequence similarity to C. pelargi, strain 812CHT is also a representative of another novel species.
31310200	15	96	theme	30629T=CCM	2345:2354	arg1	8833T					2356:2360	=LMG 30629T=CCM 8833T	2340:2360	=LMG 30629T=CCM 8833T	2340:2360	In conclusion, three novel species of the genus Corynebacterium are proposed, namely Corynebacteriumpseudopelargi 812CHT (=LMG 30627T=CCM 8832T), Corynebacterium choanae 200CHT (=LMG 30628T=CCM 8831T) and Corynebacteriumgerontici W8T (=LMG 30629T=CCM 8833T), respectively.
31310200	15	96	theme	30629T=CCM	2345:2354	arg1	812CHT					2219:2224	Corynebacteriumpseudopelargi 812CHT	2190:2224	Corynebacteriumpseudopelargi 812CHT (=LMG 30627T=CCM 8832T)	2190:2248	In conclusion, three novel species of the genus Corynebacterium are proposed, namely Corynebacteriumpseudopelargi 812CHT (=LMG 30627T=CCM 8832T), Corynebacterium choanae 200CHT (=LMG 30628T=CCM 8831T) and Corynebacteriumgerontici W8T (=LMG 30629T=CCM 8833T), respectively.
31310200	9	97	theme	81.6	1299:1302	arg1	%					1313:1313	81.6 and 87.4 %	1299:1313	%	1313:1313	In both, fusA gene and corresponding protein sequence strain 200CHT showed low sequence similarities to C. epidermidicanis 410T(81.6 and 87.4 %, respectively).
31310200	10	98	dep	Strains	1331:1337	arg1	Strains					1331:1337	Strains	1331:1337	Strains 812CHT and W8T	1331:1352	Strains 812CHT and W8T had 76.7 % ANI similarity to each other and 88.2 and 76.4 % to C. pelargi 136/3T, respectively.
31310200	10	98	dep	Strains	1331:1337	arg1	W8T					1350:1352	W8T	1350:1352	W8T	1350:1352	Strains 812CHT and W8T had 76.7 % ANI similarity to each other and 88.2 and 76.4 % to C. pelargi 136/3T, respectively.
31310200	10	98	dep	Strains	1331:1337	arg1	812CHT					1339:1344	812CHT	1339:1344	812CHT	1339:1344	Strains 812CHT and W8T had 76.7 % ANI similarity to each other and 88.2 and 76.4 % to C. pelargi 136/3T, respectively.
31310200	6	99	theme	pseudotuberculosis	767:784	arg1	102968T					790:796	Corynebacterium pseudotuberculosis CIP 102968T	751:796	Corynebacterium pseudotuberculosis CIP 102968T	751:796	The 16S rRNA gene sequence of 200CHT showed highest similarities to Corynebacterium epidermidicanis 410T (96.7 %) followed by Corynebacterium argentoratense DSM 44202T, Corynebacterium ulcerans NCTC 7910T and Corynebacterium pseudotuberculosis CIP 102968T (each 96.3 %).
31310200	9	100	theme	87.4 	1308:1312	arg1	%					1313:1313	81.6 and 87.4 %	1299:1313	%	1313:1313	In both, fusA gene and corresponding protein sequence strain 200CHT showed low sequence similarities to C. epidermidicanis 410T(81.6 and 87.4 %, respectively).
31310200	4	101	theme	non-motile	304:313	arg1	strains					325:331	Three Gram-stain-positive, rod-to-coccoid-shaped, catalase-positive and non-motile bacterial strains	232:331	Three Gram-stain-positive, rod-to-coccoid-shaped, catalase-positive and non-motile bacterial strains isolated from the choanae of a Northern bald ibis	232:381	Three Gram-stain-positive, rod-to-coccoid-shaped, catalase-positive and non-motile bacterial strains isolated from the choanae of a Northern bald ibis, designated strains 200CHT, W8T and 812CHT, respectively, were subjected to comprehensive taxonomic characterization.
31310200	10	102	theme	pelargi	1420:1426	arg1	136/3T					1428:1433	C. pelargi 136/3T	1417:1433	C. pelargi 136/3T	1417:1433	Strains 812CHT and W8T had 76.7 % ANI similarity to each other and 88.2 and 76.4 % to C. pelargi 136/3T, respectively.
31310200	15	103	theme	=LMG	2340:2343	arg1	8833T					2356:2360	=LMG 30629T=CCM 8833T	2340:2360	=LMG 30629T=CCM 8833T	2340:2360	In conclusion, three novel species of the genus Corynebacterium are proposed, namely Corynebacteriumpseudopelargi 812CHT (=LMG 30627T=CCM 8832T), Corynebacterium choanae 200CHT (=LMG 30628T=CCM 8831T) and Corynebacteriumgerontici W8T (=LMG 30629T=CCM 8833T), respectively.
31310200	15	103	theme	=LMG	2340:2343	arg1	812CHT					2219:2224	Corynebacteriumpseudopelargi 812CHT	2190:2224	Corynebacteriumpseudopelargi 812CHT (=LMG 30627T=CCM 8832T)	2190:2248	In conclusion, three novel species of the genus Corynebacterium are proposed, namely Corynebacteriumpseudopelargi 812CHT (=LMG 30627T=CCM 8832T), Corynebacterium choanae 200CHT (=LMG 30628T=CCM 8831T) and Corynebacteriumgerontici W8T (=LMG 30629T=CCM 8833T), respectively.
31310200	6	104	theme	NCTC	736:739	arg1	7910T					741:745	Corynebacterium ulcerans NCTC 7910T	711:745	Corynebacterium ulcerans NCTC 7910T	711:745	The 16S rRNA gene sequence of 200CHT showed highest similarities to Corynebacterium epidermidicanis 410T (96.7 %) followed by Corynebacterium argentoratense DSM 44202T, Corynebacterium ulcerans NCTC 7910T and Corynebacterium pseudotuberculosis CIP 102968T (each 96.3 %).
31310200	0	105	theme	corynebacterial	24:38	arg1	strains					40:46	three corynebacterial strains	18:46	three corynebacterial strains isolated from the Northern Bald Ibis (Geronticus eremita)	18:104	Classification of three corynebacterial strains isolated from the Northern Bald Ibis (Geronticus eremita): proposal of Corynebacterium choanae sp.
31310200	7	106	theme	16S	856:858	arg1	rRNA					860:863	16S rRNA	856:863	highest 16S rRNA gene sequence similarities to Corynebacterium pelargi 136/3T (98.0 and 99.9 %, respectively)	848:956	Strains W8T and 812CHT both showed highest 16S rRNA gene sequence similarities to Corynebacterium pelargi 136/3T (98.0 and 99.9 %, respectively).
31310200	4	107	theme	rod-to-coccoid-shaped	259:279	arg1	strains					325:331	Three Gram-stain-positive, rod-to-coccoid-shaped, catalase-positive and non-motile bacterial strains	232:331	Three Gram-stain-positive, rod-to-coccoid-shaped, catalase-positive and non-motile bacterial strains isolated from the choanae of a Northern bald ibis	232:381	Three Gram-stain-positive, rod-to-coccoid-shaped, catalase-positive and non-motile bacterial strains isolated from the choanae of a Northern bald ibis, designated strains 200CHT, W8T and 812CHT, respectively, were subjected to comprehensive taxonomic characterization.
31310200	6	108	theme	Corynebacterium	711:725	arg1	7910T					741:745	Corynebacterium ulcerans NCTC 7910T	711:745	Corynebacterium ulcerans NCTC 7910T	711:745	The 16S rRNA gene sequence of 200CHT showed highest similarities to Corynebacterium epidermidicanis 410T (96.7 %) followed by Corynebacterium argentoratense DSM 44202T, Corynebacterium ulcerans NCTC 7910T and Corynebacterium pseudotuberculosis CIP 102968T (each 96.3 %).
31310200	15	109	theme	Corynebacteriumpseudopelargi	2190:2217	arg1	8832T					2243:2247	=LMG 30627T=CCM 8832T	2227:2247	=LMG 30627T=CCM 8832T	2227:2247	In conclusion, three novel species of the genus Corynebacterium are proposed, namely Corynebacteriumpseudopelargi 812CHT (=LMG 30627T=CCM 8832T), Corynebacterium choanae 200CHT (=LMG 30628T=CCM 8831T) and Corynebacteriumgerontici W8T (=LMG 30629T=CCM 8833T), respectively.
31310200	15	109	theme	Corynebacteriumpseudopelargi	2190:2217	arg1	8833T					2356:2360	=LMG 30629T=CCM 8833T	2340:2360	=LMG 30629T=CCM 8833T	2340:2360	In conclusion, three novel species of the genus Corynebacterium are proposed, namely Corynebacteriumpseudopelargi 812CHT (=LMG 30627T=CCM 8832T), Corynebacterium choanae 200CHT (=LMG 30628T=CCM 8831T) and Corynebacteriumgerontici W8T (=LMG 30629T=CCM 8833T), respectively.
31310200	15	109	theme	Corynebacteriumpseudopelargi	2190:2217	arg1	812CHT					2219:2224	Corynebacteriumpseudopelargi 812CHT	2190:2224	Corynebacteriumpseudopelargi 812CHT (=LMG 30627T=CCM 8832T)	2190:2248	In conclusion, three novel species of the genus Corynebacterium are proposed, namely Corynebacteriumpseudopelargi 812CHT (=LMG 30627T=CCM 8832T), Corynebacterium choanae 200CHT (=LMG 30628T=CCM 8831T) and Corynebacteriumgerontici W8T (=LMG 30629T=CCM 8833T), respectively.
31310200	15	110	dep	Corynebacterium	2251:2265	arg1	choanae					2267:2273	choanae	2267:2273	choanae	2267:2273	In conclusion, three novel species of the genus Corynebacterium are proposed, namely Corynebacteriumpseudopelargi 812CHT (=LMG 30627T=CCM 8832T), Corynebacterium choanae 200CHT (=LMG 30628T=CCM 8831T) and Corynebacteriumgerontici W8T (=LMG 30629T=CCM 8833T), respectively.
31310200	4	111	theme	catalase-positive	282:298	arg1	strains					325:331	Three Gram-stain-positive, rod-to-coccoid-shaped, catalase-positive and non-motile bacterial strains	232:331	Three Gram-stain-positive, rod-to-coccoid-shaped, catalase-positive and non-motile bacterial strains isolated from the choanae of a Northern bald ibis	232:381	Three Gram-stain-positive, rod-to-coccoid-shaped, catalase-positive and non-motile bacterial strains isolated from the choanae of a Northern bald ibis, designated strains 200CHT, W8T and 812CHT, respectively, were subjected to comprehensive taxonomic characterization.
31310200	11	112	theme	C.	1565:1566	arg1	136/3T					1576:1581	C. pelargi 136/3T	1565:1581	C. pelargi 136/3T	1565:1581	In silico DNA-DNA hybridization values for 812CHT and W8T were 22.1 % among the two strains and 35.3 and 21.7 % to C. pelargi 136/3T, respectively.
31310200	13	113	theme	diamino	1926:1932	arg1	acid					1934:1937	the diagnostic diamino acid	1911:1937	the diagnostic diamino acid of the peptidoglycan	1911:1958	All three strains possessed corynemycolic acids and contained meso-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan.
31310200	13	113	theme	diamino	1926:1932	arg1	acid					1903:1906	contained meso-diaminopimelic acid	1873:1906	contained meso-diaminopimelic acid	1873:1906	All three strains possessed corynemycolic acids and contained meso-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan.
31310200	13	113	theme	diamino	1926:1932	arg1	acids					1863:1867	corynemycolic acids	1849:1867	corynemycolic acids	1849:1867	All three strains possessed corynemycolic acids and contained meso-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan.
31310200	12	114	theme	C.	1744:1745	arg1	pelargi					1747:1753	C. pelargi	1744:1753	C. pelargi	1744:1753	These data not only demonstrate that strain W8T is a representative of a novel species, but despite the high 16S rRNA gene sequence similarity to C. pelargi, strain 812CHT is also a representative of another novel species.
31310200	11	115	theme	In	1450:1451	arg1	hybridization					1468:1480	In silico DNA-DNA hybridization	1450:1480	In silico DNA-DNA hybridization values for 812CHT and W8T	1450:1506	In silico DNA-DNA hybridization values for 812CHT and W8T were 22.1 % among the two strains and 35.3 and 21.7 % to C. pelargi 136/3T, respectively.
31310200	12	116	theme	rRNA	1711:1714	arg1	similarity					1730:1739	the high 16S rRNA gene sequence similarity	1698:1739	the high 16S rRNA gene sequence similarity to C. pelargi	1698:1753	These data not only demonstrate that strain W8T is a representative of a novel species, but despite the high 16S rRNA gene sequence similarity to C. pelargi, strain 812CHT is also a representative of another novel species.
31310200	2	117	theme	Corynebacterium	197:211	arg1	gerontici					213:221	Corynebacterium gerontici	197:221	Corynebacterium gerontici	197:221	nov., and Corynebacterium gerontici sp.
31310200	10	118	contain	had	1354:1356	arg2	similarity					1369:1378	76.7 % ANI similarity	1358:1378	76.7 % ANI similarity to each other	1358:1392	Strains 812CHT and W8T had 76.7 % ANI similarity to each other and 88.2 and 76.4 % to C. pelargi 136/3T, respectively.
31310200	10	118	contain	had	1354:1356	arg1	Strains					1331:1337	Strains	1331:1337	Strains 812CHT and W8T	1331:1352	Strains 812CHT and W8T had 76.7 % ANI similarity to each other and 88.2 and 76.4 % to C. pelargi 136/3T, respectively.
31310200	10	118	contain	had	1354:1356	arg2	%					1412:1412	88.2 and 76.4 %	1398:1412	88.2 and 76.4 % to C. pelargi 136/3T	1398:1433	Strains 812CHT and W8T had 76.7 % ANI similarity to each other and 88.2 and 76.4 % to C. pelargi 136/3T, respectively.
31310200	10	118	contain	had	1354:1356	arg1	812CHT					1339:1344	812CHT	1339:1344	812CHT	1339:1344	Strains 812CHT and W8T had 76.7 % ANI similarity to each other and 88.2 and 76.4 % to C. pelargi 136/3T, respectively.
31310200	10	118	contain	had	1354:1356	arg1	W8T					1350:1352	W8T	1350:1352	W8T	1350:1352	Strains 812CHT and W8T had 76.7 % ANI similarity to each other and 88.2 and 76.4 % to C. pelargi 136/3T, respectively.
31310200	4	119	theme	bald	373:376	arg1	ibis					378:381	a Northern bald ibis	362:381	a Northern bald ibis	362:381	Three Gram-stain-positive, rod-to-coccoid-shaped, catalase-positive and non-motile bacterial strains isolated from the choanae of a Northern bald ibis, designated strains 200CHT, W8T and 812CHT, respectively, were subjected to comprehensive taxonomic characterization.
31310200	12	120	theme	16S	1707:1709	arg1	similarity					1730:1739	the high 16S rRNA gene sequence similarity	1698:1739	the high 16S rRNA gene sequence similarity to C. pelargi	1698:1753	These data not only demonstrate that strain W8T is a representative of a novel species, but despite the high 16S rRNA gene sequence similarity to C. pelargi, strain 812CHT is also a representative of another novel species.
31310200	8	121	theme	partial	977:983	arg1	sequence					1003:1010	the partial housekeeping gene sequence	973:1010	the partial housekeeping gene sequence of fusA	973:1018	Comparison of the partial housekeeping gene sequence of fusA showed higher sequence similarities of 812CHT to C. pelargi (95.8 %) than W8T (90.9 %) which was also confirmed by corresponding amino acid sequences.
31310200	6	122	theme	200CHT	572:577	arg1	sequence					560:567	The 16S rRNA gene sequence	542:567	The 16S rRNA gene sequence of 200CHT	542:577	The 16S rRNA gene sequence of 200CHT showed highest similarities to Corynebacterium epidermidicanis 410T (96.7 %) followed by Corynebacterium argentoratense DSM 44202T, Corynebacterium ulcerans NCTC 7910T and Corynebacterium pseudotuberculosis CIP 102968T (each 96.3 %).
31310200	11	123	theme	pelargi	1568:1574	arg1	136/3T					1576:1581	C. pelargi 136/3T	1565:1581	C. pelargi 136/3T	1565:1581	In silico DNA-DNA hybridization values for 812CHT and W8T were 22.1 % among the two strains and 35.3 and 21.7 % to C. pelargi 136/3T, respectively.
31310200	15	124	theme	Corynebacterium	2251:2265	arg1	8831T					2299:2303	=LMG 30628T=CCM 8831T	2283:2303	=LMG 30628T=CCM 8831T	2283:2303	In conclusion, three novel species of the genus Corynebacterium are proposed, namely Corynebacteriumpseudopelargi 812CHT (=LMG 30627T=CCM 8832T), Corynebacterium choanae 200CHT (=LMG 30628T=CCM 8831T) and Corynebacteriumgerontici W8T (=LMG 30629T=CCM 8833T), respectively.
31310200	15	124	theme	Corynebacterium	2251:2265	arg1	200CHT					2275:2280	Corynebacterium choanae 200CHT	2251:2280	Corynebacterium choanae 200CHT (=LMG 30628T=CCM 8831T)	2251:2304	In conclusion, three novel species of the genus Corynebacterium are proposed, namely Corynebacteriumpseudopelargi 812CHT (=LMG 30627T=CCM 8832T), Corynebacterium choanae 200CHT (=LMG 30628T=CCM 8831T) and Corynebacteriumgerontici W8T (=LMG 30629T=CCM 8833T), respectively.
31106862	11	0	theme	nanoemulsions	1924:1936	arg1	encapsulation					1904:1916	The encapsulation	1900:1916	The encapsulation of EO nanoemulsions into biopolymeric support	1900:1962	The encapsulation of EO nanoemulsions into biopolymeric support could be used for bioactive packaging to prevent food spoilage and extend shelf life.
31106862	2	1	from	toxic	496:500	arg1	bioassays					448:456	bioassays	448:456	bioassays	448:456	In bioassays, all individual and combined EOs were toxic to the rice weevil.
31106862	3	2	theme	100	611:613	arg1	%					614:614	%	614:614	%	614:614	Eucalyptus EO exhibited the highest toxicity among the individual EO treatments, causing 100% mortality at a minimum concentration of 0.8 µL/mL after 24 hr of exposure.
31106862	4	3	theme	higher	751:756	arg1	activity					767:774	higher fumigant activity	751:774	higher fumigant activity than the individual oregano or thyme treatments	751:822	The combination treatment of oregano and thyme EO displayed higher fumigant activity than the individual oregano or thyme treatments.
31106862	8	4	contain	containing	1406:1415	arg2	PLA/CNC					1484:1490	PLA/CNC	1484:1490	PLA/CNC	1484:1490	Incorporating an oregano:thyme nanoemulsion (0.75%) into cellulose nanocrystal (CNC) containing chitosan (CH/CNC), methyl cellulose (MC/CNC), and polylactic acid (PLA/CNC) composite films resulted in extended diffusion matrices causing 32% to 51% rice weevil mortality after 14 days exposure.
31106862	8	4	contain	containing	1406:1415	arg1	CNC					1401:1403	CNC	1401:1403	CNC	1401:1403	Incorporating an oregano:thyme nanoemulsion (0.75%) into cellulose nanocrystal (CNC) containing chitosan (CH/CNC), methyl cellulose (MC/CNC), and polylactic acid (PLA/CNC) composite films resulted in extended diffusion matrices causing 32% to 51% rice weevil mortality after 14 days exposure.
31106862	8	4	contain	containing	1406:1415	arg2	chitosan					1417:1424	chitosan	1417:1424	chitosan (CH/CNC)	1417:1433	Incorporating an oregano:thyme nanoemulsion (0.75%) into cellulose nanocrystal (CNC) containing chitosan (CH/CNC), methyl cellulose (MC/CNC), and polylactic acid (PLA/CNC) composite films resulted in extended diffusion matrices causing 32% to 51% rice weevil mortality after 14 days exposure.
31106862	8	4	contain	containing	1406:1415	arg2	CH/CNC					1427:1432	CH/CNC	1427:1432	CH/CNC	1427:1432	Incorporating an oregano:thyme nanoemulsion (0.75%) into cellulose nanocrystal (CNC) containing chitosan (CH/CNC), methyl cellulose (MC/CNC), and polylactic acid (PLA/CNC) composite films resulted in extended diffusion matrices causing 32% to 51% rice weevil mortality after 14 days exposure.
31106862	8	4	contain	containing	1406:1415	arg2	MC/CNC					1454:1459	MC/CNC	1454:1459	MC/CNC	1454:1459	Incorporating an oregano:thyme nanoemulsion (0.75%) into cellulose nanocrystal (CNC) containing chitosan (CH/CNC), methyl cellulose (MC/CNC), and polylactic acid (PLA/CNC) composite films resulted in extended diffusion matrices causing 32% to 51% rice weevil mortality after 14 days exposure.
31106862	8	4	contain	containing	1406:1415	arg1	nanocrystal					1388:1398	cellulose nanocrystal	1378:1398	cellulose nanocrystal (CNC) containing chitosan (CH/CNC), methyl cellulose (MC/CNC), and polylactic acid (PLA/CNC) composite films	1378:1507	Incorporating an oregano:thyme nanoemulsion (0.75%) into cellulose nanocrystal (CNC) containing chitosan (CH/CNC), methyl cellulose (MC/CNC), and polylactic acid (PLA/CNC) composite films resulted in extended diffusion matrices causing 32% to 51% rice weevil mortality after 14 days exposure.
31106862	8	4	contain	containing	1406:1415	arg2	acid					1478:1481	polylactic acid	1467:1481	polylactic acid (PLA/CNC)	1467:1491	Incorporating an oregano:thyme nanoemulsion (0.75%) into cellulose nanocrystal (CNC) containing chitosan (CH/CNC), methyl cellulose (MC/CNC), and polylactic acid (PLA/CNC) composite films resulted in extended diffusion matrices causing 32% to 51% rice weevil mortality after 14 days exposure.
31106862	8	4	contain	containing	1406:1415	arg2	cellulose					1443:1451	methyl cellulose	1436:1451	methyl cellulose (MC/CNC)	1436:1460	Incorporating an oregano:thyme nanoemulsion (0.75%) into cellulose nanocrystal (CNC) containing chitosan (CH/CNC), methyl cellulose (MC/CNC), and polylactic acid (PLA/CNC) composite films resulted in extended diffusion matrices causing 32% to 51% rice weevil mortality after 14 days exposure.
31106862	1	5	theme	weevil	399:404	arg1	control					379:385	control	379:385	control of the rice weevil, Sitophilus oryzae,	379:424	The fumigant toxicity of eight individual essential oils (EOs; basil, cinnamon, eucalyptus, mandarin, oregano, peppermint, tea tree, and thyme) and one binary combination (thyme and oregano) for control of the rice weevil, Sitophilus oryzae, were investigated.
31106862	9	6	from	Gray	1633:1636	arg1	Irradiation					1614:1624	Irradiation	1614:1624	Irradiation at 200 Gray alone	1614:1642	Irradiation at 200 Gray alone caused 79% mortality and increased to 100% when combined with the bioactive chitosan film containing the oregano:thyme nanoemulsion.
31106862	11	7	theme	biopolymeric	1943:1954	arg1	support					1956:1962	biopolymeric support	1943:1962	biopolymeric support	1943:1962	The encapsulation of EO nanoemulsions into biopolymeric support could be used for bioactive packaging to prevent food spoilage and extend shelf life.
31106862	7	8	theme	MPa	1299:1301	arg1	conditions					1172:1181	The optimum conditions	1160:1181	The optimum conditions for preparing the mixture of oregano and thyme EO nanoemulsions	1160:1245	The optimum conditions for preparing the mixture of oregano and thyme EO nanoemulsions were evaluated to be homogenization pressure of 103 MPa and three cycles.
31106862	7	8	theme	MPa	1299:1301	arg1	pressure					1283:1290	homogenization pressure	1268:1290	homogenization pressure of 103 MPa and three cycles	1268:1318	The optimum conditions for preparing the mixture of oregano and thyme EO nanoemulsions were evaluated to be homogenization pressure of 103 MPa and three cycles.
31106862	8	9	theme	%	1566:1566	arg1	mortality					1580:1588	51% rice weevil mortality	1564:1588	51% rice weevil mortality after 14 days exposure	1564:1611	Incorporating an oregano:thyme nanoemulsion (0.75%) into cellulose nanocrystal (CNC) containing chitosan (CH/CNC), methyl cellulose (MC/CNC), and polylactic acid (PLA/CNC) composite films resulted in extended diffusion matrices causing 32% to 51% rice weevil mortality after 14 days exposure.
31106862	9	10	theme	oregano	1749:1755	arg1	nanoemulsion					1763:1774	the oregano:thyme nanoemulsion	1745:1774	the oregano:thyme nanoemulsion	1745:1774	Irradiation at 200 Gray alone caused 79% mortality and increased to 100% when combined with the bioactive chitosan film containing the oregano:thyme nanoemulsion.
31106862	13	11	theme	stored	2307:2312	arg1	pests					2314:2318	stored pests	2307:2318	stored pests	2307:2318	The system developed in this research may also be extended to explore other food-packaging films with various food models to control different types of stored pests.
31106862	1	12	theme	binary	336:341	arg1	combination					343:353	one binary combination	332:353	one binary combination (thyme and oregano)	332:373	The fumigant toxicity of eight individual essential oils (EOs; basil, cinnamon, eucalyptus, mandarin, oregano, peppermint, tea tree, and thyme) and one binary combination (thyme and oregano) for control of the rice weevil, Sitophilus oryzae, were investigated.
31106862	8	13	theme	weevil	1573:1578	arg1	mortality					1580:1588	51% rice weevil mortality	1564:1588	51% rice weevil mortality after 14 days exposure	1564:1611	Incorporating an oregano:thyme nanoemulsion (0.75%) into cellulose nanocrystal (CNC) containing chitosan (CH/CNC), methyl cellulose (MC/CNC), and polylactic acid (PLA/CNC) composite films resulted in extended diffusion matrices causing 32% to 51% rice weevil mortality after 14 days exposure.
31106862	7	14	theme	homogenization	1268:1281	arg1	conditions					1172:1181	The optimum conditions	1160:1181	The optimum conditions for preparing the mixture of oregano and thyme EO nanoemulsions	1160:1245	The optimum conditions for preparing the mixture of oregano and thyme EO nanoemulsions were evaluated to be homogenization pressure of 103 MPa and three cycles.
31106862	7	14	theme	homogenization	1268:1281	arg1	pressure					1283:1290	homogenization pressure	1268:1290	homogenization pressure of 103 MPa and three cycles	1268:1318	The optimum conditions for preparing the mixture of oregano and thyme EO nanoemulsions were evaluated to be homogenization pressure of 103 MPa and three cycles.
31106862	9	15	theme	thyme	1757:1761	arg1	nanoemulsion					1763:1774	the oregano:thyme nanoemulsion	1745:1774	the oregano:thyme nanoemulsion	1745:1774	Irradiation at 200 Gray alone caused 79% mortality and increased to 100% when combined with the bioactive chitosan film containing the oregano:thyme nanoemulsion.
31106862	3	16	theme	exposure	681:688	arg1	hr					675:676	24 hr	672:676	24 hr of exposure	672:688	Eucalyptus EO exhibited the highest toxicity among the individual EO treatments, causing 100% mortality at a minimum concentration of 0.8 µL/mL after 24 hr of exposure.
31106862	0	17	theme	Weevil	141:146	arg1	Control					125:131	Control	125:131	Control of Rice Weevil	125:146	Synergistic Effects of Nanocomposite Films Containing Essential Oil Nanoemulsions in Combination with Ionizing Radiation for Control of Rice Weevil Sitophilus oryzae in Stored Grains.
31106862	3	18	theme	Eucalyptus	522:531	arg1	EO					533:534	Eucalyptus EO	522:534	Eucalyptus EO	522:534	Eucalyptus EO exhibited the highest toxicity among the individual EO treatments, causing 100% mortality at a minimum concentration of 0.8 µL/mL after 24 hr of exposure.
31106862	1	19	theme	essential	226:234	arg1	oils					236:239	eight individual essential oils	209:239	eight individual essential oils (EOs; basil, cinnamon, eucalyptus, mandarin, oregano, peppermint, tea tree, and thyme)	209:326	The fumigant toxicity of eight individual essential oils (EOs; basil, cinnamon, eucalyptus, mandarin, oregano, peppermint, tea tree, and thyme) and one binary combination (thyme and oregano) for control of the rice weevil, Sitophilus oryzae, were investigated.
31106862	1	19	theme	essential	226:234	arg1	thyme					321:325	thyme	321:325	thyme	321:325	The fumigant toxicity of eight individual essential oils (EOs; basil, cinnamon, eucalyptus, mandarin, oregano, peppermint, tea tree, and thyme) and one binary combination (thyme and oregano) for control of the rice weevil, Sitophilus oryzae, were investigated.
31106862	1	19	theme	essential	226:234	arg1	EOs					242:244	EOs	242:244	EOs	242:244	The fumigant toxicity of eight individual essential oils (EOs; basil, cinnamon, eucalyptus, mandarin, oregano, peppermint, tea tree, and thyme) and one binary combination (thyme and oregano) for control of the rice weevil, Sitophilus oryzae, were investigated.
31106862	1	19	theme	essential	226:234	arg1	eucalyptus					264:273	eucalyptus	264:273	eucalyptus	264:273	The fumigant toxicity of eight individual essential oils (EOs; basil, cinnamon, eucalyptus, mandarin, oregano, peppermint, tea tree, and thyme) and one binary combination (thyme and oregano) for control of the rice weevil, Sitophilus oryzae, were investigated.
31106862	1	19	theme	essential	226:234	arg1	mandarin					276:283	mandarin	276:283	mandarin	276:283	The fumigant toxicity of eight individual essential oils (EOs; basil, cinnamon, eucalyptus, mandarin, oregano, peppermint, tea tree, and thyme) and one binary combination (thyme and oregano) for control of the rice weevil, Sitophilus oryzae, were investigated.
31106862	1	19	theme	essential	226:234	arg1	tree					311:314	tea tree	307:314	tea tree	307:314	The fumigant toxicity of eight individual essential oils (EOs; basil, cinnamon, eucalyptus, mandarin, oregano, peppermint, tea tree, and thyme) and one binary combination (thyme and oregano) for control of the rice weevil, Sitophilus oryzae, were investigated.
31106862	1	19	theme	essential	226:234	arg1	basil					247:251	basil	247:251	basil	247:251	The fumigant toxicity of eight individual essential oils (EOs; basil, cinnamon, eucalyptus, mandarin, oregano, peppermint, tea tree, and thyme) and one binary combination (thyme and oregano) for control of the rice weevil, Sitophilus oryzae, were investigated.
31106862	1	19	theme	essential	226:234	arg1	cinnamon					254:261	cinnamon	254:261	cinnamon	254:261	The fumigant toxicity of eight individual essential oils (EOs; basil, cinnamon, eucalyptus, mandarin, oregano, peppermint, tea tree, and thyme) and one binary combination (thyme and oregano) for control of the rice weevil, Sitophilus oryzae, were investigated.
31106862	1	19	theme	essential	226:234	arg1	oregano					286:292	oregano	286:292	oregano	286:292	The fumigant toxicity of eight individual essential oils (EOs; basil, cinnamon, eucalyptus, mandarin, oregano, peppermint, tea tree, and thyme) and one binary combination (thyme and oregano) for control of the rice weevil, Sitophilus oryzae, were investigated.
31106862	4	20	theme	thyme	807:811	arg1	treatments					813:822	the individual oregano or thyme treatments	781:822	treatments	813:822	The combination treatment of oregano and thyme EO displayed higher fumigant activity than the individual oregano or thyme treatments.
31106862	0	21	from	Nanoemulsions	68:80	arg1	Combination					85:95	Combination	85:95	Combination with Ionizing Radiation	85:119	Synergistic Effects of Nanocomposite Films Containing Essential Oil Nanoemulsions in Combination with Ionizing Radiation for Control of Rice Weevil Sitophilus oryzae in Stored Grains.
31106862	8	22	theme	days	1599:1602	arg1	exposure					1604:1611	14 days exposure	1596:1611	14 days exposure	1596:1611	Incorporating an oregano:thyme nanoemulsion (0.75%) into cellulose nanocrystal (CNC) containing chitosan (CH/CNC), methyl cellulose (MC/CNC), and polylactic acid (PLA/CNC) composite films resulted in extended diffusion matrices causing 32% to 51% rice weevil mortality after 14 days exposure.
31106862	5	23	theme	stable	827:832	arg1	nanoemulsion					847:858	A stable oil-in-water nanoemulsion	825:858	A stable oil-in-water nanoemulsion	825:858	A stable oil-in-water nanoemulsion was evaluated using high-pressure homogenization (microfluidization [MF]) and varying the pressure and number of cycles.
31106862	12	24	theme	weevil	2130:2135	arg1	control					2114:2120	complete control	2105:2120	complete control of rice weevil in packaged rice	2105:2152	Combining bioactive films with irradiation can provide complete control of rice weevil in packaged rice.
31106862	0	25	theme	Stored	169:174	arg1	Grains					176:181	Stored Grains	169:181	Stored Grains	169:181	Synergistic Effects of Nanocomposite Films Containing Essential Oil Nanoemulsions in Combination with Ionizing Radiation for Control of Rice Weevil Sitophilus oryzae in Stored Grains.
31106862	3	26	theme	highest	550:556	arg1	toxicity					558:565	the highest toxicity	546:565	the highest toxicity among the individual EO treatments	546:600	Eucalyptus EO exhibited the highest toxicity among the individual EO treatments, causing 100% mortality at a minimum concentration of 0.8 µL/mL after 24 hr of exposure.
31106862	11	27	theme	EO	1921:1922	arg1	nanoemulsions					1924:1936	EO nanoemulsions	1921:1936	EO nanoemulsions	1921:1936	The encapsulation of EO nanoemulsions into biopolymeric support could be used for bioactive packaging to prevent food spoilage and extend shelf life.
31106862	11	28	theme	shelf	2038:2042	arg1	life					2044:2047	shelf life	2038:2047	shelf life	2038:2047	The encapsulation of EO nanoemulsions into biopolymeric support could be used for bioactive packaging to prevent food spoilage and extend shelf life.
31106862	12	29	theme	packaged	2140:2147	arg1	rice					2149:2152	packaged rice	2140:2152	packaged rice	2140:2152	Combining bioactive films with irradiation can provide complete control of rice weevil in packaged rice.
31106862	7	30	theme	EO	1230:1231	arg1	nanoemulsions					1233:1245	EO nanoemulsions	1230:1245	EO nanoemulsions	1230:1245	The optimum conditions for preparing the mixture of oregano and thyme EO nanoemulsions were evaluated to be homogenization pressure of 103 MPa and three cycles.
31106862	10	31	theme	oregano	1824:1830	arg1	combination					1809:1819	A binary combination	1800:1819	PRACTICAL APPLICATION: A binary combination of oregano:thyme	1777:1836	PRACTICAL APPLICATION: A binary combination of oregano:thyme has potential as a biopesticide against stored product pests.
31106862	1	32	theme	tea	307:309	arg1	oils					236:239	eight individual essential oils	209:239	eight individual essential oils (EOs; basil, cinnamon, eucalyptus, mandarin, oregano, peppermint, tea tree, and thyme)	209:326	The fumigant toxicity of eight individual essential oils (EOs; basil, cinnamon, eucalyptus, mandarin, oregano, peppermint, tea tree, and thyme) and one binary combination (thyme and oregano) for control of the rice weevil, Sitophilus oryzae, were investigated.
31106862	1	32	theme	tea	307:309	arg1	tree					311:314	tea tree	307:314	tea tree	307:314	The fumigant toxicity of eight individual essential oils (EOs; basil, cinnamon, eucalyptus, mandarin, oregano, peppermint, tea tree, and thyme) and one binary combination (thyme and oregano) for control of the rice weevil, Sitophilus oryzae, were investigated.
31106862	11	33	used	used	1973:1976	arg2	encapsulation					1904:1916	The encapsulation	1900:1916	The encapsulation of EO nanoemulsions into biopolymeric support	1900:1962	The encapsulation of EO nanoemulsions into biopolymeric support could be used for bioactive packaging to prevent food spoilage and extend shelf life.
31106862	2	34	theme	rice	509:512	arg1	weevil					514:519	the rice weevil	505:519	the rice weevil	505:519	In bioassays, all individual and combined EOs were toxic to the rice weevil.
31106862	8	35	theme	cellulose	1378:1386	arg1	CNC					1401:1403	CNC	1401:1403	CNC	1401:1403	Incorporating an oregano:thyme nanoemulsion (0.75%) into cellulose nanocrystal (CNC) containing chitosan (CH/CNC), methyl cellulose (MC/CNC), and polylactic acid (PLA/CNC) composite films resulted in extended diffusion matrices causing 32% to 51% rice weevil mortality after 14 days exposure.
31106862	8	35	theme	cellulose	1378:1386	arg1	nanocrystal					1388:1398	cellulose nanocrystal	1378:1398	cellulose nanocrystal (CNC) containing chitosan (CH/CNC), methyl cellulose (MC/CNC), and polylactic acid (PLA/CNC) composite films	1378:1507	Incorporating an oregano:thyme nanoemulsion (0.75%) into cellulose nanocrystal (CNC) containing chitosan (CH/CNC), methyl cellulose (MC/CNC), and polylactic acid (PLA/CNC) composite films resulted in extended diffusion matrices causing 32% to 51% rice weevil mortality after 14 days exposure.
31106862	0	36	theme	Ionizing	102:109	arg1	Radiation					111:119	Ionizing Radiation	102:119	Ionizing Radiation	102:119	Synergistic Effects of Nanocomposite Films Containing Essential Oil Nanoemulsions in Combination with Ionizing Radiation for Control of Rice Weevil Sitophilus oryzae in Stored Grains.
31106862	13	37	theme	various	2257:2263	arg1	models					2270:2275	various food models	2257:2275	various food models	2257:2275	The system developed in this research may also be extended to explore other food-packaging films with various food models to control different types of stored pests.
31106862	11	38	theme	food	2013:2016	arg1	spoilage					2018:2025	food spoilage	2013:2025	food spoilage	2013:2025	The encapsulation of EO nanoemulsions into biopolymeric support could be used for bioactive packaging to prevent food spoilage and extend shelf life.
31106862	7	39	theme	thyme	1224:1228	arg1	mixture					1201:1207	the mixture	1197:1207	the mixture of oregano and thyme EO nanoemulsions	1197:1245	The optimum conditions for preparing the mixture of oregano and thyme EO nanoemulsions were evaluated to be homogenization pressure of 103 MPa and three cycles.
31106862	11	40	theme	bioactive	1982:1990	arg1	packaging					1992:2000	bioactive packaging	1982:2000	bioactive packaging	1982:2000	The encapsulation of EO nanoemulsions into biopolymeric support could be used for bioactive packaging to prevent food spoilage and extend shelf life.
31106862	0	41	theme	Synergistic	0:10	arg1	Effects					12:18	Synergistic Effects	0:18	Synergistic Effects of Nanocomposite Films	0:41	Synergistic Effects of Nanocomposite Films Containing Essential Oil Nanoemulsions in Combination with Ionizing Radiation for Control of Rice Weevil Sitophilus oryzae in Stored Grains.
31106862	13	42	theme	other	2225:2229	arg1	films					2246:2250	other food-packaging films	2225:2250	other food-packaging films with various food models	2225:2275	The system developed in this research may also be extended to explore other food-packaging films with various food models to control different types of stored pests.
31106862	7	43	theme	oregano	1212:1218	arg1	mixture					1201:1207	the mixture	1197:1207	the mixture of oregano and thyme EO nanoemulsions	1197:1245	The optimum conditions for preparing the mixture of oregano and thyme EO nanoemulsions were evaluated to be homogenization pressure of 103 MPa and three cycles.
31106862	5	44	theme	high-pressure	880:892	arg1	homogenization					894:907	high-pressure homogenization	880:907	high-pressure homogenization (microfluidization [MF])	880:932	A stable oil-in-water nanoemulsion was evaluated using high-pressure homogenization (microfluidization [MF]) and varying the pressure and number of cycles.
31106862	5	44	theme	high-pressure	880:892	arg1	[MF					928:930	microfluidization [MF	910:930	microfluidization [MF	910:930	A stable oil-in-water nanoemulsion was evaluated using high-pressure homogenization (microfluidization [MF]) and varying the pressure and number of cycles.
31106862	9	45	theme	79	1651:1652	arg1	%					1653:1653	%	1653:1653	%	1653:1653	Irradiation at 200 Gray alone caused 79% mortality and increased to 100% when combined with the bioactive chitosan film containing the oregano:thyme nanoemulsion.
31106862	9	46	theme	chitosan	1720:1727	arg1	film					1729:1732	the bioactive chitosan film	1706:1732	the bioactive chitosan film containing the oregano:thyme nanoemulsion	1706:1774	Irradiation at 200 Gray alone caused 79% mortality and increased to 100% when combined with the bioactive chitosan film containing the oregano:thyme nanoemulsion.
31106862	3	47	theme	individual	577:586	arg1	treatments					591:600	the individual EO treatments	573:600	the individual EO treatments	573:600	Eucalyptus EO exhibited the highest toxicity among the individual EO treatments, causing 100% mortality at a minimum concentration of 0.8 µL/mL after 24 hr of exposure.
31106862	0	48	theme	Films	37:41	arg1	Effects					12:18	Synergistic Effects	0:18	Synergistic Effects of Nanocomposite Films	0:41	Synergistic Effects of Nanocomposite Films Containing Essential Oil Nanoemulsions in Combination with Ionizing Radiation for Control of Rice Weevil Sitophilus oryzae in Stored Grains.
31106862	7	49	dep	oregano	1212:1218	arg1	nanoemulsions					1233:1245	EO nanoemulsions	1230:1245	EO nanoemulsions	1230:1245	The optimum conditions for preparing the mixture of oregano and thyme EO nanoemulsions were evaluated to be homogenization pressure of 103 MPa and three cycles.
31106862	0	50	theme	Essential	54:62	arg1	Nanoemulsions					68:80	Essential Oil Nanoemulsions	54:80	Essential Oil Nanoemulsions in Combination with Ionizing Radiation for Control of Rice Weevil	54:146	Synergistic Effects of Nanocomposite Films Containing Essential Oil Nanoemulsions in Combination with Ionizing Radiation for Control of Rice Weevil Sitophilus oryzae in Stored Grains.
31106862	10	51	theme	product	1885:1891	arg1	pests					1893:1897	stored product pests	1878:1897	stored product pests	1878:1897	PRACTICAL APPLICATION: A binary combination of oregano:thyme has potential as a biopesticide against stored product pests.
31106862	4	52	theme	combination	695:705	arg1	treatment					707:715	The combination treatment	691:715	The combination treatment of oregano and thyme EO	691:739	The combination treatment of oregano and thyme EO displayed higher fumigant activity than the individual oregano or thyme treatments.
31106862	1	53	theme	oils	236:239	arg1	toxicity					197:204	The fumigant toxicity	184:204	The fumigant toxicity of eight individual essential oils (EOs; basil, cinnamon, eucalyptus, mandarin, oregano, peppermint, tea tree, and thyme) and one binary combination (thyme and oregano) for control of the rice weevil, Sitophilus oryzae,	184:424	The fumigant toxicity of eight individual essential oils (EOs; basil, cinnamon, eucalyptus, mandarin, oregano, peppermint, tea tree, and thyme) and one binary combination (thyme and oregano) for control of the rice weevil, Sitophilus oryzae, were investigated.
31106862	6	54	theme	droplet	985:991	arg1	size					993:996	The droplet size	981:996	The droplet size of the emulsions	981:1013	The droplet size of the emulsions was found to decrease from 217 to 71 nm and encapsulation efficiency increased from 37% to 84% with increasing MF pressure and number of cycles.
31106862	5	55	theme	varying	938:944	arg1	pressure					950:957	pressure	950:957	pressure	950:957	A stable oil-in-water nanoemulsion was evaluated using high-pressure homogenization (microfluidization [MF]) and varying the pressure and number of cycles.
31106862	1	56	dep	combination	343:353	arg1	oregano					366:372	oregano	366:372	oregano	366:372	The fumigant toxicity of eight individual essential oils (EOs; basil, cinnamon, eucalyptus, mandarin, oregano, peppermint, tea tree, and thyme) and one binary combination (thyme and oregano) for control of the rice weevil, Sitophilus oryzae, were investigated.
31106862	1	56	dep	combination	343:353	arg1	thyme					356:360	thyme	356:360	thyme	356:360	The fumigant toxicity of eight individual essential oils (EOs; basil, cinnamon, eucalyptus, mandarin, oregano, peppermint, tea tree, and thyme) and one binary combination (thyme and oregano) for control of the rice weevil, Sitophilus oryzae, were investigated.
31106862	12	57	theme	complete	2105:2112	arg1	control					2114:2120	complete control	2105:2120	complete control of rice weevil in packaged rice	2105:2152	Combining bioactive films with irradiation can provide complete control of rice weevil in packaged rice.
31106862	6	58	theme	emulsions	1005:1013	arg1	size					993:996	The droplet size	981:996	The droplet size of the emulsions	981:1013	The droplet size of the emulsions was found to decrease from 217 to 71 nm and encapsulation efficiency increased from 37% to 84% with increasing MF pressure and number of cycles.
31106862	8	59	theme	polylactic	1467:1476	arg1	acid					1478:1481	polylactic acid	1467:1481	polylactic acid (PLA/CNC)	1467:1491	Incorporating an oregano:thyme nanoemulsion (0.75%) into cellulose nanocrystal (CNC) containing chitosan (CH/CNC), methyl cellulose (MC/CNC), and polylactic acid (PLA/CNC) composite films resulted in extended diffusion matrices causing 32% to 51% rice weevil mortality after 14 days exposure.
31106862	8	59	theme	polylactic	1467:1476	arg1	PLA/CNC					1484:1490	PLA/CNC	1484:1490	PLA/CNC	1484:1490	Incorporating an oregano:thyme nanoemulsion (0.75%) into cellulose nanocrystal (CNC) containing chitosan (CH/CNC), methyl cellulose (MC/CNC), and polylactic acid (PLA/CNC) composite films resulted in extended diffusion matrices causing 32% to 51% rice weevil mortality after 14 days exposure.
31106862	3	60	theme	%	614:614	arg1	mortality					616:624	100% mortality	611:624	100% mortality	611:624	Eucalyptus EO exhibited the highest toxicity among the individual EO treatments, causing 100% mortality at a minimum concentration of 0.8 µL/mL after 24 hr of exposure.
31106862	0	61	with	Combination	85:95	arg1	Radiation					111:119	Ionizing Radiation	102:119	Ionizing Radiation	102:119	Synergistic Effects of Nanocomposite Films Containing Essential Oil Nanoemulsions in Combination with Ionizing Radiation for Control of Rice Weevil Sitophilus oryzae in Stored Grains.
31106862	8	62	theme	diffusion	1530:1538	arg1	matrices					1540:1547	extended diffusion matrices	1521:1547	extended diffusion matrices causing 32% to 51% rice weevil mortality after 14 days exposure	1521:1611	Incorporating an oregano:thyme nanoemulsion (0.75%) into cellulose nanocrystal (CNC) containing chitosan (CH/CNC), methyl cellulose (MC/CNC), and polylactic acid (PLA/CNC) composite films resulted in extended diffusion matrices causing 32% to 51% rice weevil mortality after 14 days exposure.
31106862	6	63	theme	encapsulation	1059:1071	arg1	efficiency					1073:1082	encapsulation efficiency	1059:1082	encapsulation efficiency	1059:1082	The droplet size of the emulsions was found to decrease from 217 to 71 nm and encapsulation efficiency increased from 37% to 84% with increasing MF pressure and number of cycles.
31106862	4	64	theme	individual	785:794	arg1	oregano					796:802	the individual oregano or thyme treatments	781:822	oregano	796:802	The combination treatment of oregano and thyme EO displayed higher fumigant activity than the individual oregano or thyme treatments.
31106862	9	65	theme	bioactive	1710:1718	arg1	film					1729:1732	the bioactive chitosan film	1706:1732	the bioactive chitosan film containing the oregano:thyme nanoemulsion	1706:1774	Irradiation at 200 Gray alone caused 79% mortality and increased to 100% when combined with the bioactive chitosan film containing the oregano:thyme nanoemulsion.
31106862	6	66	theme	cycles	1152:1157	arg1	number					1142:1147	number	1142:1147	number of cycles	1142:1157	The droplet size of the emulsions was found to decrease from 217 to 71 nm and encapsulation efficiency increased from 37% to 84% with increasing MF pressure and number of cycles.
31106862	6	66	theme	cycles	1152:1157	arg1	pressure					1129:1136	MF pressure	1126:1136	MF pressure	1126:1136	The droplet size of the emulsions was found to decrease from 217 to 71 nm and encapsulation efficiency increased from 37% to 84% with increasing MF pressure and number of cycles.
31106862	1	67	theme	rice	394:397	arg1	weevil					399:404	the rice weevil	390:404	the rice weevil	390:404	The fumigant toxicity of eight individual essential oils (EOs; basil, cinnamon, eucalyptus, mandarin, oregano, peppermint, tea tree, and thyme) and one binary combination (thyme and oregano) for control of the rice weevil, Sitophilus oryzae, were investigated.
31106862	1	67	theme	rice	394:397	arg1	oryzae					418:423	Sitophilus oryzae	407:423	Sitophilus oryzae	407:423	The fumigant toxicity of eight individual essential oils (EOs; basil, cinnamon, eucalyptus, mandarin, oregano, peppermint, tea tree, and thyme) and one binary combination (thyme and oregano) for control of the rice weevil, Sitophilus oryzae, were investigated.
31106862	7	68	theme	cycles	1313:1318	arg1	conditions					1172:1181	The optimum conditions	1160:1181	The optimum conditions for preparing the mixture of oregano and thyme EO nanoemulsions	1160:1245	The optimum conditions for preparing the mixture of oregano and thyme EO nanoemulsions were evaluated to be homogenization pressure of 103 MPa and three cycles.
31106862	7	68	theme	cycles	1313:1318	arg1	pressure					1283:1290	homogenization pressure	1268:1290	homogenization pressure of 103 MPa and three cycles	1268:1318	The optimum conditions for preparing the mixture of oregano and thyme EO nanoemulsions were evaluated to be homogenization pressure of 103 MPa and three cycles.
31106862	6	69	dep	71	1049:1050	arg1	to					1046:1047	to	1046:1047	to	1046:1047	The droplet size of the emulsions was found to decrease from 217 to 71 nm and encapsulation efficiency increased from 37% to 84% with increasing MF pressure and number of cycles.
31106862	3	70	theme	minimum	631:637	arg1	concentration					639:651	a minimum concentration	629:651	a minimum concentration of 0.8 µL/mL after 24 hr of exposure	629:688	Eucalyptus EO exhibited the highest toxicity among the individual EO treatments, causing 100% mortality at a minimum concentration of 0.8 µL/mL after 24 hr of exposure.
31106862	1	71	theme	fumigant	188:195	arg1	toxicity					197:204	The fumigant toxicity	184:204	The fumigant toxicity of eight individual essential oils (EOs; basil, cinnamon, eucalyptus, mandarin, oregano, peppermint, tea tree, and thyme) and one binary combination (thyme and oregano) for control of the rice weevil, Sitophilus oryzae,	184:424	The fumigant toxicity of eight individual essential oils (EOs; basil, cinnamon, eucalyptus, mandarin, oregano, peppermint, tea tree, and thyme) and one binary combination (thyme and oregano) for control of the rice weevil, Sitophilus oryzae, were investigated.
31106862	13	72	with	films	2246:2250	arg1	models					2270:2275	various food models	2257:2275	various food models	2257:2275	The system developed in this research may also be extended to explore other food-packaging films with various food models to control different types of stored pests.
31106862	4	73	theme	fumigant	758:765	arg1	activity					767:774	higher fumigant activity	751:774	higher fumigant activity than the individual oregano or thyme treatments	751:822	The combination treatment of oregano and thyme EO displayed higher fumigant activity than the individual oregano or thyme treatments.
31106862	5	74	dep	pressure	950:957	arg1	the					946:948	the	946:948	the	946:948	A stable oil-in-water nanoemulsion was evaluated using high-pressure homogenization (microfluidization [MF]) and varying the pressure and number of cycles.
31106862	10	75	theme	PRACTICAL	1777:1785	arg1	APPLICATION					1787:1797	PRACTICAL APPLICATION	1777:1797	PRACTICAL APPLICATION: A binary combination of oregano:thyme	1777:1836	PRACTICAL APPLICATION: A binary combination of oregano:thyme has potential as a biopesticide against stored product pests.
31106862	0	76	theme	Rice	136:139	arg1	Weevil					141:146	Rice Weevil	136:146	Rice Weevil	136:146	Synergistic Effects of Nanocomposite Films Containing Essential Oil Nanoemulsions in Combination with Ionizing Radiation for Control of Rice Weevil Sitophilus oryzae in Stored Grains.
31106862	8	77	theme	51	1564:1565	arg1	%					1566:1566	%	1566:1566	%	1566:1566	Incorporating an oregano:thyme nanoemulsion (0.75%) into cellulose nanocrystal (CNC) containing chitosan (CH/CNC), methyl cellulose (MC/CNC), and polylactic acid (PLA/CNC) composite films resulted in extended diffusion matrices causing 32% to 51% rice weevil mortality after 14 days exposure.
31106862	2	78	theme	combined	478:485	arg1	EOs					487:489	all individual and combined EOs	459:489	all individual and combined EOs	459:489	In bioassays, all individual and combined EOs were toxic to the rice weevil.
31106862	8	79	theme	oregano	1338:1344	arg1	nanoemulsion					1352:1363	an oregano:thyme nanoemulsion	1335:1363	an oregano:thyme nanoemulsion (0.75%)	1335:1371	Incorporating an oregano:thyme nanoemulsion (0.75%) into cellulose nanocrystal (CNC) containing chitosan (CH/CNC), methyl cellulose (MC/CNC), and polylactic acid (PLA/CNC) composite films resulted in extended diffusion matrices causing 32% to 51% rice weevil mortality after 14 days exposure.
31106862	8	79	theme	oregano	1338:1344	arg1	%					1370:1370	0.75%	1366:1370	0.75%	1366:1370	Incorporating an oregano:thyme nanoemulsion (0.75%) into cellulose nanocrystal (CNC) containing chitosan (CH/CNC), methyl cellulose (MC/CNC), and polylactic acid (PLA/CNC) composite films resulted in extended diffusion matrices causing 32% to 51% rice weevil mortality after 14 days exposure.
31106862	3	80	theme	µL/mL	660:664	arg1	concentration					639:651	a minimum concentration	629:651	a minimum concentration of 0.8 µL/mL after 24 hr of exposure	629:688	Eucalyptus EO exhibited the highest toxicity among the individual EO treatments, causing 100% mortality at a minimum concentration of 0.8 µL/mL after 24 hr of exposure.
31106862	13	81	theme	pests	2314:2318	arg1	types					2298:2302	different types	2288:2302	different types of stored pests	2288:2318	The system developed in this research may also be extended to explore other food-packaging films with various food models to control different types of stored pests.
31106862	1	82	theme	combination	343:353	arg1	toxicity					197:204	The fumigant toxicity	184:204	The fumigant toxicity of eight individual essential oils (EOs; basil, cinnamon, eucalyptus, mandarin, oregano, peppermint, tea tree, and thyme) and one binary combination (thyme and oregano) for control of the rice weevil, Sitophilus oryzae,	184:424	The fumigant toxicity of eight individual essential oils (EOs; basil, cinnamon, eucalyptus, mandarin, oregano, peppermint, tea tree, and thyme) and one binary combination (thyme and oregano) for control of the rice weevil, Sitophilus oryzae, were investigated.
31106862	8	83	theme	rice	1568:1571	arg1	mortality					1580:1588	51% rice weevil mortality	1564:1588	51% rice weevil mortality after 14 days exposure	1564:1611	Incorporating an oregano:thyme nanoemulsion (0.75%) into cellulose nanocrystal (CNC) containing chitosan (CH/CNC), methyl cellulose (MC/CNC), and polylactic acid (PLA/CNC) composite films resulted in extended diffusion matrices causing 32% to 51% rice weevil mortality after 14 days exposure.
31106862	1	84	theme	individual	215:224	arg1	oils					236:239	eight individual essential oils	209:239	eight individual essential oils (EOs; basil, cinnamon, eucalyptus, mandarin, oregano, peppermint, tea tree, and thyme)	209:326	The fumigant toxicity of eight individual essential oils (EOs; basil, cinnamon, eucalyptus, mandarin, oregano, peppermint, tea tree, and thyme) and one binary combination (thyme and oregano) for control of the rice weevil, Sitophilus oryzae, were investigated.
31106862	1	84	theme	individual	215:224	arg1	thyme					321:325	thyme	321:325	thyme	321:325	The fumigant toxicity of eight individual essential oils (EOs; basil, cinnamon, eucalyptus, mandarin, oregano, peppermint, tea tree, and thyme) and one binary combination (thyme and oregano) for control of the rice weevil, Sitophilus oryzae, were investigated.
31106862	1	84	theme	individual	215:224	arg1	EOs					242:244	EOs	242:244	EOs	242:244	The fumigant toxicity of eight individual essential oils (EOs; basil, cinnamon, eucalyptus, mandarin, oregano, peppermint, tea tree, and thyme) and one binary combination (thyme and oregano) for control of the rice weevil, Sitophilus oryzae, were investigated.
31106862	1	84	theme	individual	215:224	arg1	eucalyptus					264:273	eucalyptus	264:273	eucalyptus	264:273	The fumigant toxicity of eight individual essential oils (EOs; basil, cinnamon, eucalyptus, mandarin, oregano, peppermint, tea tree, and thyme) and one binary combination (thyme and oregano) for control of the rice weevil, Sitophilus oryzae, were investigated.
31106862	1	84	theme	individual	215:224	arg1	mandarin					276:283	mandarin	276:283	mandarin	276:283	The fumigant toxicity of eight individual essential oils (EOs; basil, cinnamon, eucalyptus, mandarin, oregano, peppermint, tea tree, and thyme) and one binary combination (thyme and oregano) for control of the rice weevil, Sitophilus oryzae, were investigated.
31106862	1	84	theme	individual	215:224	arg1	tree					311:314	tea tree	307:314	tea tree	307:314	The fumigant toxicity of eight individual essential oils (EOs; basil, cinnamon, eucalyptus, mandarin, oregano, peppermint, tea tree, and thyme) and one binary combination (thyme and oregano) for control of the rice weevil, Sitophilus oryzae, were investigated.
31106862	1	84	theme	individual	215:224	arg1	basil					247:251	basil	247:251	basil	247:251	The fumigant toxicity of eight individual essential oils (EOs; basil, cinnamon, eucalyptus, mandarin, oregano, peppermint, tea tree, and thyme) and one binary combination (thyme and oregano) for control of the rice weevil, Sitophilus oryzae, were investigated.
31106862	1	84	theme	individual	215:224	arg1	cinnamon					254:261	cinnamon	254:261	cinnamon	254:261	The fumigant toxicity of eight individual essential oils (EOs; basil, cinnamon, eucalyptus, mandarin, oregano, peppermint, tea tree, and thyme) and one binary combination (thyme and oregano) for control of the rice weevil, Sitophilus oryzae, were investigated.
31106862	1	84	theme	individual	215:224	arg1	oregano					286:292	oregano	286:292	oregano	286:292	The fumigant toxicity of eight individual essential oils (EOs; basil, cinnamon, eucalyptus, mandarin, oregano, peppermint, tea tree, and thyme) and one binary combination (thyme and oregano) for control of the rice weevil, Sitophilus oryzae, were investigated.
31106862	2	85	theme	individual	463:472	arg1	EOs					487:489	all individual and combined EOs	459:489	all individual and combined EOs	459:489	In bioassays, all individual and combined EOs were toxic to the rice weevil.
31106862	1	86	dep	oils	236:239	arg1	oils					236:239	eight individual essential oils	209:239	eight individual essential oils (EOs; basil, cinnamon, eucalyptus, mandarin, oregano, peppermint, tea tree, and thyme)	209:326	The fumigant toxicity of eight individual essential oils (EOs; basil, cinnamon, eucalyptus, mandarin, oregano, peppermint, tea tree, and thyme) and one binary combination (thyme and oregano) for control of the rice weevil, Sitophilus oryzae, were investigated.
31106862	1	86	dep	oils	236:239	arg1	peppermint					295:304	peppermint	295:304	peppermint	295:304	The fumigant toxicity of eight individual essential oils (EOs; basil, cinnamon, eucalyptus, mandarin, oregano, peppermint, tea tree, and thyme) and one binary combination (thyme and oregano) for control of the rice weevil, Sitophilus oryzae, were investigated.
31106862	1	86	dep	oils	236:239	arg1	thyme					321:325	thyme	321:325	thyme	321:325	The fumigant toxicity of eight individual essential oils (EOs; basil, cinnamon, eucalyptus, mandarin, oregano, peppermint, tea tree, and thyme) and one binary combination (thyme and oregano) for control of the rice weevil, Sitophilus oryzae, were investigated.
31106862	1	86	dep	oils	236:239	arg1	EOs					242:244	EOs	242:244	EOs	242:244	The fumigant toxicity of eight individual essential oils (EOs; basil, cinnamon, eucalyptus, mandarin, oregano, peppermint, tea tree, and thyme) and one binary combination (thyme and oregano) for control of the rice weevil, Sitophilus oryzae, were investigated.
31106862	1	86	dep	oils	236:239	arg1	eucalyptus					264:273	eucalyptus	264:273	eucalyptus	264:273	The fumigant toxicity of eight individual essential oils (EOs; basil, cinnamon, eucalyptus, mandarin, oregano, peppermint, tea tree, and thyme) and one binary combination (thyme and oregano) for control of the rice weevil, Sitophilus oryzae, were investigated.
31106862	1	86	dep	oils	236:239	arg1	mandarin					276:283	mandarin	276:283	mandarin	276:283	The fumigant toxicity of eight individual essential oils (EOs; basil, cinnamon, eucalyptus, mandarin, oregano, peppermint, tea tree, and thyme) and one binary combination (thyme and oregano) for control of the rice weevil, Sitophilus oryzae, were investigated.
31106862	1	86	dep	oils	236:239	arg1	tree					311:314	tea tree	307:314	tea tree	307:314	The fumigant toxicity of eight individual essential oils (EOs; basil, cinnamon, eucalyptus, mandarin, oregano, peppermint, tea tree, and thyme) and one binary combination (thyme and oregano) for control of the rice weevil, Sitophilus oryzae, were investigated.
31106862	1	86	dep	oils	236:239	arg1	basil					247:251	basil	247:251	basil	247:251	The fumigant toxicity of eight individual essential oils (EOs; basil, cinnamon, eucalyptus, mandarin, oregano, peppermint, tea tree, and thyme) and one binary combination (thyme and oregano) for control of the rice weevil, Sitophilus oryzae, were investigated.
31106862	1	86	dep	oils	236:239	arg1	cinnamon					254:261	cinnamon	254:261	cinnamon	254:261	The fumigant toxicity of eight individual essential oils (EOs; basil, cinnamon, eucalyptus, mandarin, oregano, peppermint, tea tree, and thyme) and one binary combination (thyme and oregano) for control of the rice weevil, Sitophilus oryzae, were investigated.
31106862	1	86	dep	oils	236:239	arg1	oregano					286:292	oregano	286:292	oregano	286:292	The fumigant toxicity of eight individual essential oils (EOs; basil, cinnamon, eucalyptus, mandarin, oregano, peppermint, tea tree, and thyme) and one binary combination (thyme and oregano) for control of the rice weevil, Sitophilus oryzae, were investigated.
31106862	6	87	theme	MF	1126:1127	arg1	pressure					1129:1136	MF pressure	1126:1136	MF pressure	1126:1136	The droplet size of the emulsions was found to decrease from 217 to 71 nm and encapsulation efficiency increased from 37% to 84% with increasing MF pressure and number of cycles.
31106862	10	88	theme	binary	1802:1807	arg1	combination					1809:1819	A binary combination	1800:1819	PRACTICAL APPLICATION: A binary combination of oregano:thyme	1777:1836	PRACTICAL APPLICATION: A binary combination of oregano:thyme has potential as a biopesticide against stored product pests.
31106862	2	89	from	bioassays	448:456	arg1	toxic					496:500	toxic	496:500	toxic	496:500	In bioassays, all individual and combined EOs were toxic to the rice weevil.
31106862	13	90	theme	different	2288:2296	arg1	types					2298:2302	different types	2288:2302	different types of stored pests	2288:2318	The system developed in this research may also be extended to explore other food-packaging films with various food models to control different types of stored pests.
31106862	5	91	theme	cycles	973:978	arg1	pressure					950:957	pressure	950:957	pressure	950:957	A stable oil-in-water nanoemulsion was evaluated using high-pressure homogenization (microfluidization [MF]) and varying the pressure and number of cycles.
31106862	5	91	theme	cycles	973:978	arg1	[MF					928:930	microfluidization [MF	910:930	microfluidization [MF	910:930	A stable oil-in-water nanoemulsion was evaluated using high-pressure homogenization (microfluidization [MF]) and varying the pressure and number of cycles.
31106862	5	91	theme	cycles	973:978	arg1	homogenization					894:907	high-pressure homogenization	880:907	high-pressure homogenization (microfluidization [MF])	880:932	A stable oil-in-water nanoemulsion was evaluated using high-pressure homogenization (microfluidization [MF]) and varying the pressure and number of cycles.
31106862	5	91	theme	cycles	973:978	arg1	number					963:968	number	963:968	number	963:968	A stable oil-in-water nanoemulsion was evaluated using high-pressure homogenization (microfluidization [MF]) and varying the pressure and number of cycles.
31106862	5	92	theme	oil-in-water	834:845	arg1	nanoemulsion					847:858	A stable oil-in-water nanoemulsion	825:858	A stable oil-in-water nanoemulsion	825:858	A stable oil-in-water nanoemulsion was evaluated using high-pressure homogenization (microfluidization [MF]) and varying the pressure and number of cycles.
31106862	8	93	dep	chitosan	1417:1424	arg1	films					1503:1507	composite films	1493:1507	composite films	1493:1507	Incorporating an oregano:thyme nanoemulsion (0.75%) into cellulose nanocrystal (CNC) containing chitosan (CH/CNC), methyl cellulose (MC/CNC), and polylactic acid (PLA/CNC) composite films resulted in extended diffusion matrices causing 32% to 51% rice weevil mortality after 14 days exposure.
31106862	12	94	from	control	2114:2120	arg1	rice					2149:2152	packaged rice	2140:2152	packaged rice	2140:2152	Combining bioactive films with irradiation can provide complete control of rice weevil in packaged rice.
31106862	12	95	theme	rice	2125:2128	arg1	weevil					2130:2135	rice weevil	2125:2135	rice weevil	2125:2135	Combining bioactive films with irradiation can provide complete control of rice weevil in packaged rice.
31106862	5	96	theme	microfluidization	910:926	arg1	homogenization					894:907	high-pressure homogenization	880:907	high-pressure homogenization (microfluidization [MF])	880:932	A stable oil-in-water nanoemulsion was evaluated using high-pressure homogenization (microfluidization [MF]) and varying the pressure and number of cycles.
31106862	5	96	theme	microfluidization	910:926	arg1	[MF					928:930	microfluidization [MF	910:930	microfluidization [MF	910:930	A stable oil-in-water nanoemulsion was evaluated using high-pressure homogenization (microfluidization [MF]) and varying the pressure and number of cycles.
31106862	10	97	dep	APPLICATION	1787:1797	arg1	thyme					1832:1836	thyme	1832:1836	PRACTICAL APPLICATION: A binary combination of oregano:thyme	1777:1836	PRACTICAL APPLICATION: A binary combination of oregano:thyme has potential as a biopesticide against stored product pests.
31106862	10	97	dep	APPLICATION	1787:1797	arg1	combination					1809:1819	A binary combination	1800:1819	PRACTICAL APPLICATION: A binary combination of oregano:thyme	1777:1836	PRACTICAL APPLICATION: A binary combination of oregano:thyme has potential as a biopesticide against stored product pests.
31106862	10	98	contain	has	1838:1840	arg2	biopesticide					1857:1868	a biopesticide	1855:1868	a biopesticide against stored product pests	1855:1897	PRACTICAL APPLICATION: A binary combination of oregano:thyme has potential as a biopesticide against stored product pests.
31106862	10	98	contain	has	1838:1840	arg2	potential					1842:1850	potential	1842:1850	potential	1842:1850	PRACTICAL APPLICATION: A binary combination of oregano:thyme has potential as a biopesticide against stored product pests.
31106862	10	98	contain	has	1838:1840	arg1	APPLICATION					1787:1797	PRACTICAL APPLICATION	1777:1797	PRACTICAL APPLICATION: A binary combination of oregano:thyme	1777:1836	PRACTICAL APPLICATION: A binary combination of oregano:thyme has potential as a biopesticide against stored product pests.
31106862	12	99	theme	bioactive	2060:2068	arg1	films					2070:2074	bioactive films	2060:2074	bioactive films	2060:2074	Combining bioactive films with irradiation can provide complete control of rice weevil in packaged rice.
31106862	0	100	theme	Nanocomposite	23:35	arg1	Films					37:41	Nanocomposite Films	23:41	Nanocomposite Films	23:41	Synergistic Effects of Nanocomposite Films Containing Essential Oil Nanoemulsions in Combination with Ionizing Radiation for Control of Rice Weevil Sitophilus oryzae in Stored Grains.
31106862	13	101	theme	food-packaging	2231:2244	arg1	films					2246:2250	other food-packaging films	2225:2250	other food-packaging films with various food models	2225:2275	The system developed in this research may also be extended to explore other food-packaging films with various food models to control different types of stored pests.
31106862	13	102	theme	food	2265:2268	arg1	models					2270:2275	various food models	2257:2275	various food models	2257:2275	The system developed in this research may also be extended to explore other food-packaging films with various food models to control different types of stored pests.
31106862	9	103	theme	%	1653:1653	arg1	mortality					1655:1663	79% mortality	1651:1663	79% mortality	1651:1663	Irradiation at 200 Gray alone caused 79% mortality and increased to 100% when combined with the bioactive chitosan film containing the oregano:thyme nanoemulsion.
31106862	8	104	theme	methyl	1436:1441	arg1	MC/CNC					1454:1459	MC/CNC	1454:1459	MC/CNC	1454:1459	Incorporating an oregano:thyme nanoemulsion (0.75%) into cellulose nanocrystal (CNC) containing chitosan (CH/CNC), methyl cellulose (MC/CNC), and polylactic acid (PLA/CNC) composite films resulted in extended diffusion matrices causing 32% to 51% rice weevil mortality after 14 days exposure.
31106862	8	104	theme	methyl	1436:1441	arg1	cellulose					1443:1451	methyl cellulose	1436:1451	methyl cellulose (MC/CNC)	1436:1460	Incorporating an oregano:thyme nanoemulsion (0.75%) into cellulose nanocrystal (CNC) containing chitosan (CH/CNC), methyl cellulose (MC/CNC), and polylactic acid (PLA/CNC) composite films resulted in extended diffusion matrices causing 32% to 51% rice weevil mortality after 14 days exposure.
31106862	4	105	theme	oregano	720:726	arg1	treatment					707:715	The combination treatment	691:715	The combination treatment of oregano and thyme EO	691:739	The combination treatment of oregano and thyme EO displayed higher fumigant activity than the individual oregano or thyme treatments.
31106862	0	106	theme	Oil	64:66	arg1	Nanoemulsions					68:80	Essential Oil Nanoemulsions	54:80	Essential Oil Nanoemulsions in Combination with Ionizing Radiation for Control of Rice Weevil	54:146	Synergistic Effects of Nanocomposite Films Containing Essential Oil Nanoemulsions in Combination with Ionizing Radiation for Control of Rice Weevil Sitophilus oryzae in Stored Grains.
31106862	7	107	theme	optimum	1164:1170	arg1	pressure					1283:1290	homogenization pressure	1268:1290	homogenization pressure of 103 MPa and three cycles	1268:1318	The optimum conditions for preparing the mixture of oregano and thyme EO nanoemulsions were evaluated to be homogenization pressure of 103 MPa and three cycles.
31106862	7	107	theme	optimum	1164:1170	arg1	conditions					1172:1181	The optimum conditions	1160:1181	The optimum conditions for preparing the mixture of oregano and thyme EO nanoemulsions	1160:1245	The optimum conditions for preparing the mixture of oregano and thyme EO nanoemulsions were evaluated to be homogenization pressure of 103 MPa and three cycles.
31106862	4	108	theme	thyme	732:736	arg1	treatment					707:715	The combination treatment	691:715	The combination treatment of oregano and thyme EO	691:739	The combination treatment of oregano and thyme EO displayed higher fumigant activity than the individual oregano or thyme treatments.
31106862	8	109	theme	thyme	1346:1350	arg1	nanoemulsion					1352:1363	an oregano:thyme nanoemulsion	1335:1363	an oregano:thyme nanoemulsion (0.75%)	1335:1371	Incorporating an oregano:thyme nanoemulsion (0.75%) into cellulose nanocrystal (CNC) containing chitosan (CH/CNC), methyl cellulose (MC/CNC), and polylactic acid (PLA/CNC) composite films resulted in extended diffusion matrices causing 32% to 51% rice weevil mortality after 14 days exposure.
31106862	8	109	theme	thyme	1346:1350	arg1	%					1370:1370	0.75%	1366:1370	0.75%	1366:1370	Incorporating an oregano:thyme nanoemulsion (0.75%) into cellulose nanocrystal (CNC) containing chitosan (CH/CNC), methyl cellulose (MC/CNC), and polylactic acid (PLA/CNC) composite films resulted in extended diffusion matrices causing 32% to 51% rice weevil mortality after 14 days exposure.
31106862	8	110	theme	composite	1493:1501	arg1	films					1503:1507	composite films	1493:1507	composite films	1493:1507	Incorporating an oregano:thyme nanoemulsion (0.75%) into cellulose nanocrystal (CNC) containing chitosan (CH/CNC), methyl cellulose (MC/CNC), and polylactic acid (PLA/CNC) composite films resulted in extended diffusion matrices causing 32% to 51% rice weevil mortality after 14 days exposure.
31106862	10	111	theme	stored	1878:1883	arg1	pests					1893:1897	stored product pests	1878:1897	stored product pests	1878:1897	PRACTICAL APPLICATION: A binary combination of oregano:thyme has potential as a biopesticide against stored product pests.
31106862	3	112	theme	EO	588:589	arg1	treatments					591:600	the individual EO treatments	573:600	the individual EO treatments	573:600	Eucalyptus EO exhibited the highest toxicity among the individual EO treatments, causing 100% mortality at a minimum concentration of 0.8 µL/mL after 24 hr of exposure.
31106862	4	113	dep	oregano	720:726	arg1	EO					738:739	EO	738:739	EO	738:739	The combination treatment of oregano and thyme EO displayed higher fumigant activity than the individual oregano or thyme treatments.
31106862	9	114	contain	containing	1734:1743	arg1	film					1729:1732	the bioactive chitosan film	1706:1732	the bioactive chitosan film containing the oregano:thyme nanoemulsion	1706:1774	Irradiation at 200 Gray alone caused 79% mortality and increased to 100% when combined with the bioactive chitosan film containing the oregano:thyme nanoemulsion.
31106862	9	114	contain	containing	1734:1743	arg2	nanoemulsion					1763:1774	the oregano:thyme nanoemulsion	1745:1774	the oregano:thyme nanoemulsion	1745:1774	Irradiation at 200 Gray alone caused 79% mortality and increased to 100% when combined with the bioactive chitosan film containing the oregano:thyme nanoemulsion.
31106862	8	115	theme	extended	1521:1528	arg1	matrices					1540:1547	extended diffusion matrices	1521:1547	extended diffusion matrices causing 32% to 51% rice weevil mortality after 14 days exposure	1521:1611	Incorporating an oregano:thyme nanoemulsion (0.75%) into cellulose nanocrystal (CNC) containing chitosan (CH/CNC), methyl cellulose (MC/CNC), and polylactic acid (PLA/CNC) composite films resulted in extended diffusion matrices causing 32% to 51% rice weevil mortality after 14 days exposure.
30560446	8	0	theme	relaxation	1742:1751	arg1	times					1753:1757	T1ρ relaxation times	1738:1757	T1ρ relaxation times	1738:1757	Univariate linear regression models were used to determine the association between quadriceps strength and T1ρ relaxation times in the entire weight-bearing MFC and LFC, as well as the ROI in each respective limb.
30560446	3	1	theme	medial	758:763	arg1	condyles					785:792	the medial and lateral femoral condyles	754:792	the medial and lateral femoral condyles	754:792	The purpose of the cross-sectional study was to determine if quadriceps strength was associated with T1ρ relaxation times, a marker of proteoglycan density, of the articular cartilage in the medial and lateral femoral condyles 6 months following ACLR.
30560446	3	2	theme	cross-sectional	586:600	arg1	study					602:606	the cross-sectional study	582:606	the cross-sectional study	582:606	The purpose of the cross-sectional study was to determine if quadriceps strength was associated with T1ρ relaxation times, a marker of proteoglycan density, of the articular cartilage in the medial and lateral femoral condyles 6 months following ACLR.
30560446	7	3	theme	corresponding	1570:1582	arg1	anatomy					1593:1599	the corresponding meniscal anatomy	1566:1599	the corresponding meniscal anatomy viewed in the sagittal plane	1566:1628	Following image registration, the articular cartilage for the weight-bearing surfaces of the medial and lateral femoral condyles (MFC and LFC) were manually segmented and further sub-sectioned into posterior, central and anterior regions of interest (ROI) based on the corresponding meniscal anatomy viewed in the sagittal plane.
30560446	10	4	theme	central-MFC	2353:2363	arg1	ROI					2365:2367	central-MFC ROI	2353:2367	central-MFC ROI	2353:2367	A post hoc analysis found lesser strength and greater T1ρ relaxation times were significantly associated in a subsection of participants (n = 18) without a concomitant medial tibiofemoral compartment meniscal or chondral injury in the entire weight-bearing MFC, as well as anterior-MFC and central-MFC ROI of the ACLR and uninjured limb.
30560446	7	5	theme	interest	1542:1549	arg1	regions					1531:1537	posterior, central and anterior regions	1499:1537	posterior, central and anterior regions of interest (ROI)	1499:1555	Following image registration, the articular cartilage for the weight-bearing surfaces of the medial and lateral femoral condyles (MFC and LFC) were manually segmented and further sub-sectioned into posterior, central and anterior regions of interest (ROI) based on the corresponding meniscal anatomy viewed in the sagittal plane.
30560446	11	6	theme	lower	2520:2524	arg1	strength					2543:2550	lower extremity muscle strength	2520:2550	lower extremity muscle strength	2520:2550	CONCLUSIONS The association between weaker quadriceps and greater T1ρ relaxation times in the MFC suggests deficits in lower extremity muscle strength may be related to cartilage composition as early as 6 months following ACLR.
30560446	11	7	dep	CONCLUSIONS	2401:2411	arg1	suggests					2499:2506	suggests	2499:2506	suggests deficits in lower extremity muscle strength may be related to cartilage composition as early as 6 months following ACLR	2499:2626	CONCLUSIONS The association between weaker quadriceps and greater T1ρ relaxation times in the MFC suggests deficits in lower extremity muscle strength may be related to cartilage composition as early as 6 months following ACLR.
30560446	3	8	theme	quadriceps	628:637	arg1	strength					639:646	quadriceps strength	628:646	quadriceps strength	628:646	The purpose of the cross-sectional study was to determine if quadriceps strength was associated with T1ρ relaxation times, a marker of proteoglycan density, of the articular cartilage in the medial and lateral femoral condyles 6 months following ACLR.
30560446	1	9	theme	altered	305:311	arg1	biomechanics					329:340	altered lower extremity biomechanics	305:340	altered lower extremity biomechanics	305:340	PURPOSE Quadriceps weakness following anterior cruciate ligament reconstruction (ACLR) is linked to decreased patient-reported function, altered lower extremity biomechanics and tibiofemoral joint space narrowing.
30560446	10	10	theme	uninjured	2385:2393	arg1	limb					2395:2398	uninjured limb	2385:2398	uninjured limb	2385:2398	A post hoc analysis found lesser strength and greater T1ρ relaxation times were significantly associated in a subsection of participants (n = 18) without a concomitant medial tibiofemoral compartment meniscal or chondral injury in the entire weight-bearing MFC, as well as anterior-MFC and central-MFC ROI of the ACLR and uninjured limb.
30560446	9	11	theme	anterior-MFC	2006:2017	arg1	ROI					2019:2021	the anterior-MFC ROI	2002:2021	the anterior-MFC ROI (R2 = 0.22, P = 0.02) of the ACLR limb	2002:2060	RESULTS Lesser quadriceps strength was significantly associated with greater T1ρ relaxation times in the entire weight-bearing MFC (R2 = 0.14, P = 0.05) and the anterior-MFC ROI (R2 = 0.22, P = 0.02) of the ACLR limb.
30560446	9	11	theme	anterior-MFC	2006:2017	arg1	P = 0.02					2035:2042	P = 0.02	2035:2042	P = 0.02	2035:2042	RESULTS Lesser quadriceps strength was significantly associated with greater T1ρ relaxation times in the entire weight-bearing MFC (R2 = 0.14, P = 0.05) and the anterior-MFC ROI (R2 = 0.22, P = 0.02) of the ACLR limb.
30560446	12	12	theme	cartilage	2727:2735	arg1	health					2737:2742	cartilage health	2727:2742	cartilage health	2727:2742	Maximizing quadriceps strength in the first 6 months following ACLR may be critical for promoting cartilage health early following ACLR.
30560446	1	13	theme	anterior	206:213	arg1	ACLR					249:252	ACLR	249:252	ACLR	249:252	PURPOSE Quadriceps weakness following anterior cruciate ligament reconstruction (ACLR) is linked to decreased patient-reported function, altered lower extremity biomechanics and tibiofemoral joint space narrowing.
30560446	1	13	theme	anterior	206:213	arg1	reconstruction					233:246	anterior cruciate ligament reconstruction	206:246	anterior cruciate ligament reconstruction (ACLR)	206:253	PURPOSE Quadriceps weakness following anterior cruciate ligament reconstruction (ACLR) is linked to decreased patient-reported function, altered lower extremity biomechanics and tibiofemoral joint space narrowing.
30560446	10	14	theme	ACLR	2376:2379	arg1	ROI					2365:2367	central-MFC ROI	2353:2367	central-MFC ROI	2353:2367	A post hoc analysis found lesser strength and greater T1ρ relaxation times were significantly associated in a subsection of participants (n = 18) without a concomitant medial tibiofemoral compartment meniscal or chondral injury in the entire weight-bearing MFC, as well as anterior-MFC and central-MFC ROI of the ACLR and uninjured limb.
30560446	10	14	theme	ACLR	2376:2379	arg1	n = 18					2201:2206	n = 18	2201:2206	n = 18	2201:2206	A post hoc analysis found lesser strength and greater T1ρ relaxation times were significantly associated in a subsection of participants (n = 18) without a concomitant medial tibiofemoral compartment meniscal or chondral injury in the entire weight-bearing MFC, as well as anterior-MFC and central-MFC ROI of the ACLR and uninjured limb.
30560446	10	14	theme	ACLR	2376:2379	arg1	participants					2187:2198	participants	2187:2198	participants (n = 18) without a concomitant medial tibiofemoral compartment meniscal or chondral injury in the entire weight-bearing MFC	2187:2322	A post hoc analysis found lesser strength and greater T1ρ relaxation times were significantly associated in a subsection of participants (n = 18) without a concomitant medial tibiofemoral compartment meniscal or chondral injury in the entire weight-bearing MFC, as well as anterior-MFC and central-MFC ROI of the ACLR and uninjured limb.
30560446	10	14	theme	ACLR	2376:2379	arg1	limb					2395:2398	uninjured limb	2385:2398	uninjured limb	2385:2398	A post hoc analysis found lesser strength and greater T1ρ relaxation times were significantly associated in a subsection of participants (n = 18) without a concomitant medial tibiofemoral compartment meniscal or chondral injury in the entire weight-bearing MFC, as well as anterior-MFC and central-MFC ROI of the ACLR and uninjured limb.
30560446	10	14	theme	ACLR	2376:2379	arg1	anterior-MFC					2336:2347	anterior-MFC	2336:2347	anterior-MFC	2336:2347	A post hoc analysis found lesser strength and greater T1ρ relaxation times were significantly associated in a subsection of participants (n = 18) without a concomitant medial tibiofemoral compartment meniscal or chondral injury in the entire weight-bearing MFC, as well as anterior-MFC and central-MFC ROI of the ACLR and uninjured limb.
30560446	10	15	theme	participants	2187:2198	arg1	subsection					2173:2182	a subsection	2171:2182	a subsection of participants (n = 18) without a concomitant medial tibiofemoral compartment meniscal or chondral injury in the entire weight-bearing MFC, as well as anterior-MFC and central-MFC ROI of the ACLR and uninjured limb	2171:2398	A post hoc analysis found lesser strength and greater T1ρ relaxation times were significantly associated in a subsection of participants (n = 18) without a concomitant medial tibiofemoral compartment meniscal or chondral injury in the entire weight-bearing MFC, as well as anterior-MFC and central-MFC ROI of the ACLR and uninjured limb.
30560446	1	16	theme	ligament	224:231	arg1	ACLR					249:252	ACLR	249:252	ACLR	249:252	PURPOSE Quadriceps weakness following anterior cruciate ligament reconstruction (ACLR) is linked to decreased patient-reported function, altered lower extremity biomechanics and tibiofemoral joint space narrowing.
30560446	1	16	theme	ligament	224:231	arg1	reconstruction					233:246	anterior cruciate ligament reconstruction	206:246	anterior cruciate ligament reconstruction (ACLR)	206:253	PURPOSE Quadriceps weakness following anterior cruciate ligament reconstruction (ACLR) is linked to decreased patient-reported function, altered lower extremity biomechanics and tibiofemoral joint space narrowing.
30560446	10	17	theme	concomitant	2219:2229	arg1	meniscal					2263:2270	a concomitant medial tibiofemoral compartment meniscal	2217:2270	a concomitant medial tibiofemoral compartment meniscal	2217:2270	A post hoc analysis found lesser strength and greater T1ρ relaxation times were significantly associated in a subsection of participants (n = 18) without a concomitant medial tibiofemoral compartment meniscal or chondral injury in the entire weight-bearing MFC, as well as anterior-MFC and central-MFC ROI of the ACLR and uninjured limb.
30560446	4	18	with	individuals	843:853	arg1	quadriceps					867:876	weaker quadriceps	860:876	weaker quadriceps	860:876	It is hypothesized that individuals with weaker quadriceps would demonstrate lesser proteoglycan density.
30560446	0	19	theme	anterior	125:132	arg1	reconstruction					152:165	anterior cruciate ligament reconstruction	125:165	anterior cruciate ligament reconstruction	125:165	Quadriceps weakness associates with greater T1ρ relaxation time in the medial femoral articular cartilage 6 months following anterior cruciate ligament reconstruction.
30560446	13	20	theme	EVIDENCE	2775:2782	arg1	LEVEL					2766:2770	LEVEL	2766:2770	LEVEL OF EVIDENCE	2766:2782	LEVEL OF EVIDENCE Prognostic level 1.
30560446	10	21	theme	hoc	2070:2072	arg1	analysis					2074:2081	A post hoc analysis	2063:2081	A post hoc analysis	2063:2081	A post hoc analysis found lesser strength and greater T1ρ relaxation times were significantly associated in a subsection of participants (n = 18) without a concomitant medial tibiofemoral compartment meniscal or chondral injury in the entire weight-bearing MFC, as well as anterior-MFC and central-MFC ROI of the ACLR and uninjured limb.
30560446	0	22	theme	ligament	143:150	arg1	reconstruction					152:165	anterior cruciate ligament reconstruction	125:165	anterior cruciate ligament reconstruction	125:165	Quadriceps weakness associates with greater T1ρ relaxation time in the medial femoral articular cartilage 6 months following anterior cruciate ligament reconstruction.
30560446	6	23	theme	T1ρ	1204:1206	arg1	times					1219:1223	voxel T1ρ relaxation times	1198:1223	voxel T1ρ relaxation times	1198:1223	Magnetic resonance images (MRI) were collected bilaterally and voxel by voxel T1ρ relaxation times were calculated using a five-image sequence and a monoexponential equation.
30560446	8	24	from	times	1753:1757	arg1	MFC					1788:1790	the entire weight-bearing MFC	1762:1790	the entire weight-bearing MFC	1762:1790	Univariate linear regression models were used to determine the association between quadriceps strength and T1ρ relaxation times in the entire weight-bearing MFC and LFC, as well as the ROI in each respective limb.
30560446	3	25	theme	relaxation	672:681	arg1	times					683:687	T1ρ relaxation times	668:687	T1ρ relaxation times	668:687	The purpose of the cross-sectional study was to determine if quadriceps strength was associated with T1ρ relaxation times, a marker of proteoglycan density, of the articular cartilage in the medial and lateral femoral condyles 6 months following ACLR.
30560446	3	25	theme	relaxation	672:681	arg1	marker					692:697	a marker	690:697	a marker of proteoglycan density	690:721	The purpose of the cross-sectional study was to determine if quadriceps strength was associated with T1ρ relaxation times, a marker of proteoglycan density, of the articular cartilage in the medial and lateral femoral condyles 6 months following ACLR.
30560446	5	26	theme	autograft	1004:1012	arg1	ACLR					1014:1017	a patellar tendon autograft ACLR	986:1017	a patellar tendon autograft ACLR	986:1017	METHODS Twenty-seven individuals (15 females, 12 males) with a patellar tendon autograft ACLR underwent isometric quadriceps strength assessments in 90°of knee flexion during a 6-month follow-up exam.
30560446	1	27	dep	altered	305:311	arg1	lower					313:317	lower	313:317	lower	313:317	PURPOSE Quadriceps weakness following anterior cruciate ligament reconstruction (ACLR) is linked to decreased patient-reported function, altered lower extremity biomechanics and tibiofemoral joint space narrowing.
30560446	9	28	theme	limb	2057:2060	arg1	ROI					2019:2021	the anterior-MFC ROI	2002:2021	the anterior-MFC ROI (R2 = 0.22, P = 0.02) of the ACLR limb	2002:2060	RESULTS Lesser quadriceps strength was significantly associated with greater T1ρ relaxation times in the entire weight-bearing MFC (R2 = 0.14, P = 0.05) and the anterior-MFC ROI (R2 = 0.22, P = 0.02) of the ACLR limb.
30560446	9	28	theme	limb	2057:2060	arg1	P = 0.05					1988:1995	P = 0.05	1988:1995	P = 0.05	1988:1995	RESULTS Lesser quadriceps strength was significantly associated with greater T1ρ relaxation times in the entire weight-bearing MFC (R2 = 0.14, P = 0.05) and the anterior-MFC ROI (R2 = 0.22, P = 0.02) of the ACLR limb.
30560446	9	28	theme	limb	2057:2060	arg1	P = 0.02					2035:2042	P = 0.02	2035:2042	P = 0.02	2035:2042	RESULTS Lesser quadriceps strength was significantly associated with greater T1ρ relaxation times in the entire weight-bearing MFC (R2 = 0.14, P = 0.05) and the anterior-MFC ROI (R2 = 0.22, P = 0.02) of the ACLR limb.
30560446	9	28	theme	limb	2057:2060	arg1	MFC					1972:1974	the entire weight-bearing MFC	1946:1974	the entire weight-bearing MFC (R2 = 0.14, P = 0.05)	1946:1996	RESULTS Lesser quadriceps strength was significantly associated with greater T1ρ relaxation times in the entire weight-bearing MFC (R2 = 0.14, P = 0.05) and the anterior-MFC ROI (R2 = 0.22, P = 0.02) of the ACLR limb.
30560446	10	29	theme	tibiofemoral	2238:2249	arg1	meniscal					2263:2270	a concomitant medial tibiofemoral compartment meniscal	2217:2270	a concomitant medial tibiofemoral compartment meniscal	2217:2270	A post hoc analysis found lesser strength and greater T1ρ relaxation times were significantly associated in a subsection of participants (n = 18) without a concomitant medial tibiofemoral compartment meniscal or chondral injury in the entire weight-bearing MFC, as well as anterior-MFC and central-MFC ROI of the ACLR and uninjured limb.
30560446	3	30	theme	proteoglycan	702:713	arg1	density					715:721	proteoglycan density	702:721	proteoglycan density	702:721	The purpose of the cross-sectional study was to determine if quadriceps strength was associated with T1ρ relaxation times, a marker of proteoglycan density, of the articular cartilage in the medial and lateral femoral condyles 6 months following ACLR.
30560446	6	31	theme	Magnetic	1126:1133	arg1	MRI					1153:1155	MRI	1153:1155	MRI	1153:1155	Magnetic resonance images (MRI) were collected bilaterally and voxel by voxel T1ρ relaxation times were calculated using a five-image sequence and a monoexponential equation.
30560446	6	31	theme	Magnetic	1126:1133	arg1	images					1145:1150	Magnetic resonance images	1126:1150	Magnetic resonance images (MRI)	1126:1156	Magnetic resonance images (MRI) were collected bilaterally and voxel by voxel T1ρ relaxation times were calculated using a five-image sequence and a monoexponential equation.
30560446	1	32	theme	PURPOSE	168:174	arg1	weakness					187:194	PURPOSE Quadriceps weakness	168:194	PURPOSE Quadriceps weakness following anterior cruciate ligament reconstruction (ACLR)	168:253	PURPOSE Quadriceps weakness following anterior cruciate ligament reconstruction (ACLR) is linked to decreased patient-reported function, altered lower extremity biomechanics and tibiofemoral joint space narrowing.
30560446	9	33	theme	Lesser	1853:1858	arg1	strength					1871:1878	RESULTS Lesser quadriceps strength	1845:1878	RESULTS Lesser quadriceps strength	1845:1878	RESULTS Lesser quadriceps strength was significantly associated with greater T1ρ relaxation times in the entire weight-bearing MFC (R2 = 0.14, P = 0.05) and the anterior-MFC ROI (R2 = 0.22, P = 0.02) of the ACLR limb.
30560446	11	34	theme	cartilage	2570:2578	arg1	composition					2580:2590	cartilage composition	2570:2590	cartilage composition as early as 6 months following ACLR	2570:2626	CONCLUSIONS The association between weaker quadriceps and greater T1ρ relaxation times in the MFC suggests deficits in lower extremity muscle strength may be related to cartilage composition as early as 6 months following ACLR.
30560446	10	35	theme	chondral	2275:2282	arg1	injury					2284:2289	chondral injury	2275:2289	chondral injury	2275:2289	A post hoc analysis found lesser strength and greater T1ρ relaxation times were significantly associated in a subsection of participants (n = 18) without a concomitant medial tibiofemoral compartment meniscal or chondral injury in the entire weight-bearing MFC, as well as anterior-MFC and central-MFC ROI of the ACLR and uninjured limb.
30560446	10	36	from	anterior-MFC	2336:2347	arg1	MFC					2320:2322	the entire weight-bearing MFC	2294:2322	the entire weight-bearing MFC	2294:2322	A post hoc analysis found lesser strength and greater T1ρ relaxation times were significantly associated in a subsection of participants (n = 18) without a concomitant medial tibiofemoral compartment meniscal or chondral injury in the entire weight-bearing MFC, as well as anterior-MFC and central-MFC ROI of the ACLR and uninjured limb.
30560446	10	37	theme	relaxation	2121:2130	arg1	times					2132:2136	greater T1ρ relaxation times	2109:2136	greater T1ρ relaxation times	2109:2136	A post hoc analysis found lesser strength and greater T1ρ relaxation times were significantly associated in a subsection of participants (n = 18) without a concomitant medial tibiofemoral compartment meniscal or chondral injury in the entire weight-bearing MFC, as well as anterior-MFC and central-MFC ROI of the ACLR and uninjured limb.
30560446	0	38	theme	medial	71:76	arg1	cartilage					96:104	the medial femoral articular cartilage	67:104	the medial femoral articular cartilage 6 months following anterior cruciate ligament reconstruction	67:165	Quadriceps weakness associates with greater T1ρ relaxation time in the medial femoral articular cartilage 6 months following anterior cruciate ligament reconstruction.
30560446	5	39	theme	patellar	988:995	arg1	ACLR					1014:1017	a patellar tendon autograft ACLR	986:1017	a patellar tendon autograft ACLR	986:1017	METHODS Twenty-seven individuals (15 females, 12 males) with a patellar tendon autograft ACLR underwent isometric quadriceps strength assessments in 90°of knee flexion during a 6-month follow-up exam.
30560446	11	40	theme	relaxation	2471:2480	arg1	times					2482:2486	greater T1ρ relaxation times	2459:2486	greater T1ρ relaxation times	2459:2486	CONCLUSIONS The association between weaker quadriceps and greater T1ρ relaxation times in the MFC suggests deficits in lower extremity muscle strength may be related to cartilage composition as early as 6 months following ACLR.
30560446	10	41	theme	greater	2109:2115	arg1	times					2132:2136	greater T1ρ relaxation times	2109:2136	greater T1ρ relaxation times	2109:2136	A post hoc analysis found lesser strength and greater T1ρ relaxation times were significantly associated in a subsection of participants (n = 18) without a concomitant medial tibiofemoral compartment meniscal or chondral injury in the entire weight-bearing MFC, as well as anterior-MFC and central-MFC ROI of the ACLR and uninjured limb.
30560446	0	42	theme	articular	86:94	arg1	cartilage					96:104	the medial femoral articular cartilage	67:104	the medial femoral articular cartilage 6 months following anterior cruciate ligament reconstruction	67:165	Quadriceps weakness associates with greater T1ρ relaxation time in the medial femoral articular cartilage 6 months following anterior cruciate ligament reconstruction.
30560446	5	43	theme	90°of	1074:1078	arg1	flexion					1085:1091	90°of knee flexion	1074:1091	90°of knee flexion	1074:1091	METHODS Twenty-seven individuals (15 females, 12 males) with a patellar tendon autograft ACLR underwent isometric quadriceps strength assessments in 90°of knee flexion during a 6-month follow-up exam.
30560446	10	44	theme	entire	2298:2303	arg1	MFC					2320:2322	the entire weight-bearing MFC	2294:2322	the entire weight-bearing MFC	2294:2322	A post hoc analysis found lesser strength and greater T1ρ relaxation times were significantly associated in a subsection of participants (n = 18) without a concomitant medial tibiofemoral compartment meniscal or chondral injury in the entire weight-bearing MFC, as well as anterior-MFC and central-MFC ROI of the ACLR and uninjured limb.
30560446	4	45	theme	weaker	860:865	arg1	quadriceps					867:876	weaker quadriceps	860:876	weaker quadriceps	860:876	It is hypothesized that individuals with weaker quadriceps would demonstrate lesser proteoglycan density.
30560446	2	46	theme	deleterious	449:459	arg1	changes					461:467	early deleterious changes	443:467	early deleterious changes to femoral cartilage composition that are suggestive of posttraumatic osteoarthritis development	443:564	It remains unknown if quadriceps weakness is associated with early deleterious changes to femoral cartilage composition that are suggestive of posttraumatic osteoarthritis development.
30560446	8	47	theme	Univariate	1631:1640	arg1	models					1660:1665	Univariate linear regression models	1631:1665	Univariate linear regression models	1631:1665	Univariate linear regression models were used to determine the association between quadriceps strength and T1ρ relaxation times in the entire weight-bearing MFC and LFC, as well as the ROI in each respective limb.
30560446	0	48	theme	Quadriceps	0:9	arg1	weakness					11:18	Quadriceps weakness	0:18	Quadriceps weakness	0:18	Quadriceps weakness associates with greater T1ρ relaxation time in the medial femoral articular cartilage 6 months following anterior cruciate ligament reconstruction.
30560446	5	49	theme	quadriceps	1039:1048	arg1	strength					1050:1057	isometric quadriceps strength	1029:1057	isometric quadriceps strength assessments in 90°of knee flexion	1029:1091	METHODS Twenty-seven individuals (15 females, 12 males) with a patellar tendon autograft ACLR underwent isometric quadriceps strength assessments in 90°of knee flexion during a 6-month follow-up exam.
30560446	9	50	theme	T1ρ	1922:1924	arg1	times					1937:1941	greater T1ρ relaxation times	1914:1941	greater T1ρ relaxation times in the entire weight-bearing MFC (R2 = 0.14, P = 0.05) and the anterior-MFC ROI (R2 = 0.22, P = 0.02) of the ACLR limb	1914:2060	RESULTS Lesser quadriceps strength was significantly associated with greater T1ρ relaxation times in the entire weight-bearing MFC (R2 = 0.14, P = 0.05) and the anterior-MFC ROI (R2 = 0.22, P = 0.02) of the ACLR limb.
30560446	8	51	theme	regression	1649:1658	arg1	models					1660:1665	Univariate linear regression models	1631:1665	Univariate linear regression models	1631:1665	Univariate linear regression models were used to determine the association between quadriceps strength and T1ρ relaxation times in the entire weight-bearing MFC and LFC, as well as the ROI in each respective limb.
30560446	8	52	from	LFC	1796:1798	arg1	MFC					1788:1790	the entire weight-bearing MFC	1762:1790	the entire weight-bearing MFC	1762:1790	Univariate linear regression models were used to determine the association between quadriceps strength and T1ρ relaxation times in the entire weight-bearing MFC and LFC, as well as the ROI in each respective limb.
30560446	2	53	theme	cartilage	480:488	arg1	composition					490:500	femoral cartilage composition	472:500	femoral cartilage composition	472:500	It remains unknown if quadriceps weakness is associated with early deleterious changes to femoral cartilage composition that are suggestive of posttraumatic osteoarthritis development.
30560446	2	54	theme	development	554:564	arg1	suggestive					511:520	suggestive	511:520	suggestive	511:520	It remains unknown if quadriceps weakness is associated with early deleterious changes to femoral cartilage composition that are suggestive of posttraumatic osteoarthritis development.
30560446	10	55	from	ROI	2365:2367	arg1	MFC					2320:2322	the entire weight-bearing MFC	2294:2322	the entire weight-bearing MFC	2294:2322	A post hoc analysis found lesser strength and greater T1ρ relaxation times were significantly associated in a subsection of participants (n = 18) without a concomitant medial tibiofemoral compartment meniscal or chondral injury in the entire weight-bearing MFC, as well as anterior-MFC and central-MFC ROI of the ACLR and uninjured limb.
30560446	8	56	used	used	1672:1675	arg2	models					1660:1665	Univariate linear regression models	1631:1665	Univariate linear regression models	1631:1665	Univariate linear regression models were used to determine the association between quadriceps strength and T1ρ relaxation times in the entire weight-bearing MFC and LFC, as well as the ROI in each respective limb.
30560446	8	57	from	ROI	1816:1818	arg1	limb					1839:1842	each respective limb	1823:1842	each respective limb	1823:1842	Univariate linear regression models were used to determine the association between quadriceps strength and T1ρ relaxation times in the entire weight-bearing MFC and LFC, as well as the ROI in each respective limb.
30560446	0	58	theme	greater	36:42	arg1	time					59:62	greater T1ρ relaxation time	36:62	greater T1ρ relaxation time in the medial femoral articular cartilage 6 months following anterior cruciate ligament reconstruction	36:165	Quadriceps weakness associates with greater T1ρ relaxation time in the medial femoral articular cartilage 6 months following anterior cruciate ligament reconstruction.
30560446	1	59	theme	tibiofemoral	346:357	arg1	narrowing					371:379	tibiofemoral joint space narrowing	346:379	tibiofemoral joint space narrowing	346:379	PURPOSE Quadriceps weakness following anterior cruciate ligament reconstruction (ACLR) is linked to decreased patient-reported function, altered lower extremity biomechanics and tibiofemoral joint space narrowing.
30560446	12	60	from	strength	2651:2658	arg1	6 months					2673:2680	the first 6 months	2663:2680	the first 6 months following ACLR	2663:2695	Maximizing quadriceps strength in the first 6 months following ACLR may be critical for promoting cartilage health early following ACLR.
30560446	7	61	theme	femoral	1413:1419	arg1	condyles					1421:1428	the medial and lateral femoral condyles	1390:1428	the medial and lateral femoral condyles (MFC and LFC)	1390:1442	Following image registration, the articular cartilage for the weight-bearing surfaces of the medial and lateral femoral condyles (MFC and LFC) were manually segmented and further sub-sectioned into posterior, central and anterior regions of interest (ROI) based on the corresponding meniscal anatomy viewed in the sagittal plane.
30560446	7	61	theme	femoral	1413:1419	arg1	LFC					1439:1441	LFC	1439:1441	LFC	1439:1441	Following image registration, the articular cartilage for the weight-bearing surfaces of the medial and lateral femoral condyles (MFC and LFC) were manually segmented and further sub-sectioned into posterior, central and anterior regions of interest (ROI) based on the corresponding meniscal anatomy viewed in the sagittal plane.
30560446	7	61	theme	femoral	1413:1419	arg1	MFC					1431:1433	MFC	1431:1433	MFC	1431:1433	Following image registration, the articular cartilage for the weight-bearing surfaces of the medial and lateral femoral condyles (MFC and LFC) were manually segmented and further sub-sectioned into posterior, central and anterior regions of interest (ROI) based on the corresponding meniscal anatomy viewed in the sagittal plane.
30560446	2	62	theme	posttraumatic	525:537	arg1	development					554:564	posttraumatic osteoarthritis development	525:564	posttraumatic osteoarthritis development	525:564	It remains unknown if quadriceps weakness is associated with early deleterious changes to femoral cartilage composition that are suggestive of posttraumatic osteoarthritis development.
30560446	10	63	theme	compartment	2251:2261	arg1	meniscal					2263:2270	a concomitant medial tibiofemoral compartment meniscal	2217:2270	a concomitant medial tibiofemoral compartment meniscal	2217:2270	A post hoc analysis found lesser strength and greater T1ρ relaxation times were significantly associated in a subsection of participants (n = 18) without a concomitant medial tibiofemoral compartment meniscal or chondral injury in the entire weight-bearing MFC, as well as anterior-MFC and central-MFC ROI of the ACLR and uninjured limb.
30560446	0	64	theme	relaxation	48:57	arg1	time					59:62	greater T1ρ relaxation time	36:62	greater T1ρ relaxation time in the medial femoral articular cartilage 6 months following anterior cruciate ligament reconstruction	36:165	Quadriceps weakness associates with greater T1ρ relaxation time in the medial femoral articular cartilage 6 months following anterior cruciate ligament reconstruction.
30560446	1	65	theme	space	365:369	arg1	narrowing					371:379	tibiofemoral joint space narrowing	346:379	tibiofemoral joint space narrowing	346:379	PURPOSE Quadriceps weakness following anterior cruciate ligament reconstruction (ACLR) is linked to decreased patient-reported function, altered lower extremity biomechanics and tibiofemoral joint space narrowing.
30560446	9	66	theme	weight-bearing	1957:1970	arg1	P = 0.05					1988:1995	P = 0.05	1988:1995	P = 0.05	1988:1995	RESULTS Lesser quadriceps strength was significantly associated with greater T1ρ relaxation times in the entire weight-bearing MFC (R2 = 0.14, P = 0.05) and the anterior-MFC ROI (R2 = 0.22, P = 0.02) of the ACLR limb.
30560446	9	66	theme	weight-bearing	1957:1970	arg1	MFC					1972:1974	the entire weight-bearing MFC	1946:1974	the entire weight-bearing MFC (R2 = 0.14, P = 0.05)	1946:1996	RESULTS Lesser quadriceps strength was significantly associated with greater T1ρ relaxation times in the entire weight-bearing MFC (R2 = 0.14, P = 0.05) and the anterior-MFC ROI (R2 = 0.22, P = 0.02) of the ACLR limb.
30560446	10	67	theme	post	2065:2068	arg1	analysis					2074:2081	A post hoc analysis	2063:2081	A post hoc analysis	2063:2081	A post hoc analysis found lesser strength and greater T1ρ relaxation times were significantly associated in a subsection of participants (n = 18) without a concomitant medial tibiofemoral compartment meniscal or chondral injury in the entire weight-bearing MFC, as well as anterior-MFC and central-MFC ROI of the ACLR and uninjured limb.
30560446	10	68	from	participants	2187:2198	arg1	MFC					2320:2322	the entire weight-bearing MFC	2294:2322	the entire weight-bearing MFC	2294:2322	A post hoc analysis found lesser strength and greater T1ρ relaxation times were significantly associated in a subsection of participants (n = 18) without a concomitant medial tibiofemoral compartment meniscal or chondral injury in the entire weight-bearing MFC, as well as anterior-MFC and central-MFC ROI of the ACLR and uninjured limb.
30560446	5	69	from	assessments	1059:1069	arg1	flexion					1085:1091	90°of knee flexion	1074:1091	90°of knee flexion	1074:1091	METHODS Twenty-seven individuals (15 females, 12 males) with a patellar tendon autograft ACLR underwent isometric quadriceps strength assessments in 90°of knee flexion during a 6-month follow-up exam.
30560446	6	70	theme	relaxation	1208:1217	arg1	times					1219:1223	voxel T1ρ relaxation times	1198:1223	voxel T1ρ relaxation times	1198:1223	Magnetic resonance images (MRI) were collected bilaterally and voxel by voxel T1ρ relaxation times were calculated using a five-image sequence and a monoexponential equation.
30560446	10	71	from	limb	2395:2398	arg1	MFC					2320:2322	the entire weight-bearing MFC	2294:2322	the entire weight-bearing MFC	2294:2322	A post hoc analysis found lesser strength and greater T1ρ relaxation times were significantly associated in a subsection of participants (n = 18) without a concomitant medial tibiofemoral compartment meniscal or chondral injury in the entire weight-bearing MFC, as well as anterior-MFC and central-MFC ROI of the ACLR and uninjured limb.
30560446	3	72	theme	cartilage	741:749	arg1	times					683:687	T1ρ relaxation times	668:687	T1ρ relaxation times	668:687	The purpose of the cross-sectional study was to determine if quadriceps strength was associated with T1ρ relaxation times, a marker of proteoglycan density, of the articular cartilage in the medial and lateral femoral condyles 6 months following ACLR.
30560446	3	72	theme	cartilage	741:749	arg1	marker					692:697	a marker	690:697	a marker of proteoglycan density	690:721	The purpose of the cross-sectional study was to determine if quadriceps strength was associated with T1ρ relaxation times, a marker of proteoglycan density, of the articular cartilage in the medial and lateral femoral condyles 6 months following ACLR.
30560446	4	73	theme	lesser	896:901	arg1	density					916:922	lesser proteoglycan density	896:922	lesser proteoglycan density	896:922	It is hypothesized that individuals with weaker quadriceps would demonstrate lesser proteoglycan density.
30560446	3	74	theme	study	602:606	arg1	purpose					571:577	The purpose	567:577	The purpose of the cross-sectional study	567:606	The purpose of the cross-sectional study was to determine if quadriceps strength was associated with T1ρ relaxation times, a marker of proteoglycan density, of the articular cartilage in the medial and lateral femoral condyles 6 months following ACLR.
30560446	8	75	theme	weight-bearing	1773:1786	arg1	MFC					1788:1790	the entire weight-bearing MFC	1762:1790	the entire weight-bearing MFC	1762:1790	Univariate linear regression models were used to determine the association between quadriceps strength and T1ρ relaxation times in the entire weight-bearing MFC and LFC, as well as the ROI in each respective limb.
30560446	7	76	theme	meniscal	1584:1591	arg1	anatomy					1593:1599	the corresponding meniscal anatomy	1566:1599	the corresponding meniscal anatomy viewed in the sagittal plane	1566:1628	Following image registration, the articular cartilage for the weight-bearing surfaces of the medial and lateral femoral condyles (MFC and LFC) were manually segmented and further sub-sectioned into posterior, central and anterior regions of interest (ROI) based on the corresponding meniscal anatomy viewed in the sagittal plane.
30560446	6	77	theme	voxel	1198:1202	arg1	times					1219:1223	voxel T1ρ relaxation times	1198:1223	voxel T1ρ relaxation times	1198:1223	Magnetic resonance images (MRI) were collected bilaterally and voxel by voxel T1ρ relaxation times were calculated using a five-image sequence and a monoexponential equation.
30560446	11	78	theme	weaker	2437:2442	arg1	quadriceps					2444:2453	weaker quadriceps	2437:2453	weaker quadriceps	2437:2453	CONCLUSIONS The association between weaker quadriceps and greater T1ρ relaxation times in the MFC suggests deficits in lower extremity muscle strength may be related to cartilage composition as early as 6 months following ACLR.
30560446	1	79	theme	joint	359:363	arg1	narrowing					371:379	tibiofemoral joint space narrowing	346:379	tibiofemoral joint space narrowing	346:379	PURPOSE Quadriceps weakness following anterior cruciate ligament reconstruction (ACLR) is linked to decreased patient-reported function, altered lower extremity biomechanics and tibiofemoral joint space narrowing.
30560446	3	80	theme	femoral	777:783	arg1	condyles					785:792	the medial and lateral femoral condyles	754:792	the medial and lateral femoral condyles	754:792	The purpose of the cross-sectional study was to determine if quadriceps strength was associated with T1ρ relaxation times, a marker of proteoglycan density, of the articular cartilage in the medial and lateral femoral condyles 6 months following ACLR.
30560446	2	81	theme	quadriceps	404:413	arg1	weakness					415:422	quadriceps weakness	404:422	quadriceps weakness	404:422	It remains unknown if quadriceps weakness is associated with early deleterious changes to femoral cartilage composition that are suggestive of posttraumatic osteoarthritis development.
30560446	1	82	theme	patient-reported	278:293	arg1	function					295:302	decreased patient-reported function	268:302	decreased patient-reported function	268:302	PURPOSE Quadriceps weakness following anterior cruciate ligament reconstruction (ACLR) is linked to decreased patient-reported function, altered lower extremity biomechanics and tibiofemoral joint space narrowing.
30560446	9	83	from	times	1937:1941	arg1	ROI					2019:2021	the anterior-MFC ROI	2002:2021	the anterior-MFC ROI (R2 = 0.22, P = 0.02) of the ACLR limb	2002:2060	RESULTS Lesser quadriceps strength was significantly associated with greater T1ρ relaxation times in the entire weight-bearing MFC (R2 = 0.14, P = 0.05) and the anterior-MFC ROI (R2 = 0.22, P = 0.02) of the ACLR limb.
30560446	9	83	from	times	1937:1941	arg1	P = 0.05					1988:1995	P = 0.05	1988:1995	P = 0.05	1988:1995	RESULTS Lesser quadriceps strength was significantly associated with greater T1ρ relaxation times in the entire weight-bearing MFC (R2 = 0.14, P = 0.05) and the anterior-MFC ROI (R2 = 0.22, P = 0.02) of the ACLR limb.
30560446	9	83	from	times	1937:1941	arg1	P = 0.02					2035:2042	P = 0.02	2035:2042	P = 0.02	2035:2042	RESULTS Lesser quadriceps strength was significantly associated with greater T1ρ relaxation times in the entire weight-bearing MFC (R2 = 0.14, P = 0.05) and the anterior-MFC ROI (R2 = 0.22, P = 0.02) of the ACLR limb.
30560446	9	83	from	times	1937:1941	arg1	MFC					1972:1974	the entire weight-bearing MFC	1946:1974	the entire weight-bearing MFC (R2 = 0.14, P = 0.05)	1946:1996	RESULTS Lesser quadriceps strength was significantly associated with greater T1ρ relaxation times in the entire weight-bearing MFC (R2 = 0.14, P = 0.05) and the anterior-MFC ROI (R2 = 0.22, P = 0.02) of the ACLR limb.
30560446	5	84	dep	METHODS	925:931	arg1	individuals					946:956	Twenty-seven individuals	933:956	METHODS Twenty-seven individuals (15 females, 12 males) with a patellar tendon autograft ACLR	925:1017	METHODS Twenty-seven individuals (15 females, 12 males) with a patellar tendon autograft ACLR underwent isometric quadriceps strength assessments in 90°of knee flexion during a 6-month follow-up exam.
30560446	8	85	theme	T1ρ	1738:1740	arg1	times					1753:1757	T1ρ relaxation times	1738:1757	T1ρ relaxation times	1738:1757	Univariate linear regression models were used to determine the association between quadriceps strength and T1ρ relaxation times in the entire weight-bearing MFC and LFC, as well as the ROI in each respective limb.
30560446	11	86	from	association	2417:2427	arg1	MFC					2495:2497	the MFC	2491:2497	the MFC	2491:2497	CONCLUSIONS The association between weaker quadriceps and greater T1ρ relaxation times in the MFC suggests deficits in lower extremity muscle strength may be related to cartilage composition as early as 6 months following ACLR.
30560446	11	87	theme	muscle	2536:2541	arg1	strength					2543:2550	lower extremity muscle strength	2520:2550	lower extremity muscle strength	2520:2550	CONCLUSIONS The association between weaker quadriceps and greater T1ρ relaxation times in the MFC suggests deficits in lower extremity muscle strength may be related to cartilage composition as early as 6 months following ACLR.
30560446	6	88	theme	monoexponential	1275:1289	arg1	equation					1291:1298	a monoexponential equation	1273:1298	a monoexponential equation	1273:1298	Magnetic resonance images (MRI) were collected bilaterally and voxel by voxel T1ρ relaxation times were calculated using a five-image sequence and a monoexponential equation.
30560446	8	89	from	association	1694:1704	arg1	limb					1839:1842	each respective limb	1823:1842	each respective limb	1823:1842	Univariate linear regression models were used to determine the association between quadriceps strength and T1ρ relaxation times in the entire weight-bearing MFC and LFC, as well as the ROI in each respective limb.
30560446	12	90	theme	first	2667:2671	arg1	6 months					2673:2680	the first 6 months	2663:2680	the first 6 months following ACLR	2663:2695	Maximizing quadriceps strength in the first 6 months following ACLR may be critical for promoting cartilage health early following ACLR.
30560446	3	91	from	condyles	785:792	arg1	times					683:687	T1ρ relaxation times	668:687	T1ρ relaxation times	668:687	The purpose of the cross-sectional study was to determine if quadriceps strength was associated with T1ρ relaxation times, a marker of proteoglycan density, of the articular cartilage in the medial and lateral femoral condyles 6 months following ACLR.
30560446	3	91	from	condyles	785:792	arg1	marker					692:697	a marker	690:697	a marker of proteoglycan density	690:721	The purpose of the cross-sectional study was to determine if quadriceps strength was associated with T1ρ relaxation times, a marker of proteoglycan density, of the articular cartilage in the medial and lateral femoral condyles 6 months following ACLR.
30560446	9	92	theme	quadriceps	1860:1869	arg1	strength					1871:1878	RESULTS Lesser quadriceps strength	1845:1878	RESULTS Lesser quadriceps strength	1845:1878	RESULTS Lesser quadriceps strength was significantly associated with greater T1ρ relaxation times in the entire weight-bearing MFC (R2 = 0.14, P = 0.05) and the anterior-MFC ROI (R2 = 0.22, P = 0.02) of the ACLR limb.
30560446	1	93	theme	cruciate	215:222	arg1	ACLR					249:252	ACLR	249:252	ACLR	249:252	PURPOSE Quadriceps weakness following anterior cruciate ligament reconstruction (ACLR) is linked to decreased patient-reported function, altered lower extremity biomechanics and tibiofemoral joint space narrowing.
30560446	1	93	theme	cruciate	215:222	arg1	reconstruction					233:246	anterior cruciate ligament reconstruction	206:246	anterior cruciate ligament reconstruction (ACLR)	206:253	PURPOSE Quadriceps weakness following anterior cruciate ligament reconstruction (ACLR) is linked to decreased patient-reported function, altered lower extremity biomechanics and tibiofemoral joint space narrowing.
30560446	7	94	theme	anterior	1522:1529	arg1	regions					1531:1537	posterior, central and anterior regions	1499:1537	posterior, central and anterior regions of interest (ROI)	1499:1555	Following image registration, the articular cartilage for the weight-bearing surfaces of the medial and lateral femoral condyles (MFC and LFC) were manually segmented and further sub-sectioned into posterior, central and anterior regions of interest (ROI) based on the corresponding meniscal anatomy viewed in the sagittal plane.
30560446	10	95	theme	medial	2231:2236	arg1	meniscal					2263:2270	a concomitant medial tibiofemoral compartment meniscal	2217:2270	a concomitant medial tibiofemoral compartment meniscal	2217:2270	A post hoc analysis found lesser strength and greater T1ρ relaxation times were significantly associated in a subsection of participants (n = 18) without a concomitant medial tibiofemoral compartment meniscal or chondral injury in the entire weight-bearing MFC, as well as anterior-MFC and central-MFC ROI of the ACLR and uninjured limb.
30560446	0	96	theme	cruciate	134:141	arg1	reconstruction					152:165	anterior cruciate ligament reconstruction	125:165	anterior cruciate ligament reconstruction	125:165	Quadriceps weakness associates with greater T1ρ relaxation time in the medial femoral articular cartilage 6 months following anterior cruciate ligament reconstruction.
30560446	6	97	theme	five-image	1249:1258	arg1	sequence					1260:1267	a five-image sequence	1247:1267	a five-image sequence	1247:1267	Magnetic resonance images (MRI) were collected bilaterally and voxel by voxel T1ρ relaxation times were calculated using a five-image sequence and a monoexponential equation.
30560446	7	98	theme	central	1510:1516	arg1	regions					1531:1537	posterior, central and anterior regions	1499:1537	posterior, central and anterior regions of interest (ROI)	1499:1555	Following image registration, the articular cartilage for the weight-bearing surfaces of the medial and lateral femoral condyles (MFC and LFC) were manually segmented and further sub-sectioned into posterior, central and anterior regions of interest (ROI) based on the corresponding meniscal anatomy viewed in the sagittal plane.
30560446	5	99	dep	follow-up	1110:1118	arg1	exam					1120:1123	exam	1120:1123	a 6-month follow-up exam	1100:1123	METHODS Twenty-seven individuals (15 females, 12 males) with a patellar tendon autograft ACLR underwent isometric quadriceps strength assessments in 90°of knee flexion during a 6-month follow-up exam.
30560446	1	100	attach	linked	258:263	arg2	weakness					187:194	PURPOSE Quadriceps weakness	168:194	PURPOSE Quadriceps weakness following anterior cruciate ligament reconstruction (ACLR)	168:253	PURPOSE Quadriceps weakness following anterior cruciate ligament reconstruction (ACLR) is linked to decreased patient-reported function, altered lower extremity biomechanics and tibiofemoral joint space narrowing.
30560446	1	100	attach	linked	258:263	arg1	function					295:302	decreased patient-reported function	268:302	decreased patient-reported function	268:302	PURPOSE Quadriceps weakness following anterior cruciate ligament reconstruction (ACLR) is linked to decreased patient-reported function, altered lower extremity biomechanics and tibiofemoral joint space narrowing.
30560446	1	100	attach	linked	258:263	arg1	biomechanics					329:340	altered lower extremity biomechanics	305:340	altered lower extremity biomechanics	305:340	PURPOSE Quadriceps weakness following anterior cruciate ligament reconstruction (ACLR) is linked to decreased patient-reported function, altered lower extremity biomechanics and tibiofemoral joint space narrowing.
30560446	1	100	attach	linked	258:263	arg1	narrowing					371:379	tibiofemoral joint space narrowing	346:379	tibiofemoral joint space narrowing	346:379	PURPOSE Quadriceps weakness following anterior cruciate ligament reconstruction (ACLR) is linked to decreased patient-reported function, altered lower extremity biomechanics and tibiofemoral joint space narrowing.
30560446	8	101	theme	respective	1828:1837	arg1	limb					1839:1842	each respective limb	1823:1842	each respective limb	1823:1842	Univariate linear regression models were used to determine the association between quadriceps strength and T1ρ relaxation times in the entire weight-bearing MFC and LFC, as well as the ROI in each respective limb.
30560446	12	102	theme	quadriceps	2640:2649	arg1	strength					2651:2658	quadriceps strength	2640:2658	quadriceps strength in the first 6 months following ACLR	2640:2695	Maximizing quadriceps strength in the first 6 months following ACLR may be critical for promoting cartilage health early following ACLR.
30560446	10	103	theme	limb	2395:2398	arg1	subsection					2173:2182	a subsection	2171:2182	a subsection of participants (n = 18) without a concomitant medial tibiofemoral compartment meniscal or chondral injury in the entire weight-bearing MFC, as well as anterior-MFC and central-MFC ROI of the ACLR and uninjured limb	2171:2398	A post hoc analysis found lesser strength and greater T1ρ relaxation times were significantly associated in a subsection of participants (n = 18) without a concomitant medial tibiofemoral compartment meniscal or chondral injury in the entire weight-bearing MFC, as well as anterior-MFC and central-MFC ROI of the ACLR and uninjured limb.
30560446	7	104	theme	posterior	1499:1507	arg1	regions					1531:1537	posterior, central and anterior regions	1499:1537	posterior, central and anterior regions of interest (ROI)	1499:1555	Following image registration, the articular cartilage for the weight-bearing surfaces of the medial and lateral femoral condyles (MFC and LFC) were manually segmented and further sub-sectioned into posterior, central and anterior regions of interest (ROI) based on the corresponding meniscal anatomy viewed in the sagittal plane.
30560446	0	105	theme	cartilage	96:104	arg1	6 months					106:113	the medial femoral articular cartilage 6 months	67:113	the medial femoral articular cartilage 6 months following anterior cruciate ligament reconstruction	67:165	Quadriceps weakness associates with greater T1ρ relaxation time in the medial femoral articular cartilage 6 months following anterior cruciate ligament reconstruction.
30560446	3	106	theme	T1ρ	668:670	arg1	times					683:687	T1ρ relaxation times	668:687	T1ρ relaxation times	668:687	The purpose of the cross-sectional study was to determine if quadriceps strength was associated with T1ρ relaxation times, a marker of proteoglycan density, of the articular cartilage in the medial and lateral femoral condyles 6 months following ACLR.
30560446	3	106	theme	T1ρ	668:670	arg1	marker					692:697	a marker	690:697	a marker of proteoglycan density	690:721	The purpose of the cross-sectional study was to determine if quadriceps strength was associated with T1ρ relaxation times, a marker of proteoglycan density, of the articular cartilage in the medial and lateral femoral condyles 6 months following ACLR.
30560446	5	107	theme	tendon	997:1002	arg1	ACLR					1014:1017	a patellar tendon autograft ACLR	986:1017	a patellar tendon autograft ACLR	986:1017	METHODS Twenty-seven individuals (15 females, 12 males) with a patellar tendon autograft ACLR underwent isometric quadriceps strength assessments in 90°of knee flexion during a 6-month follow-up exam.
30560446	11	108	dep	early	2595:2599	arg1	as					2601:2602	as	2601:2602	as	2601:2602	CONCLUSIONS The association between weaker quadriceps and greater T1ρ relaxation times in the MFC suggests deficits in lower extremity muscle strength may be related to cartilage composition as early as 6 months following ACLR.
30560446	9	109	theme	ACLR	2052:2055	arg1	limb					2057:2060	the ACLR limb	2048:2060	the ACLR limb	2048:2060	RESULTS Lesser quadriceps strength was significantly associated with greater T1ρ relaxation times in the entire weight-bearing MFC (R2 = 0.14, P = 0.05) and the anterior-MFC ROI (R2 = 0.22, P = 0.02) of the ACLR limb.
30560446	6	110	theme	resonance	1135:1143	arg1	MRI					1153:1155	MRI	1153:1155	MRI	1153:1155	Magnetic resonance images (MRI) were collected bilaterally and voxel by voxel T1ρ relaxation times were calculated using a five-image sequence and a monoexponential equation.
30560446	6	110	theme	resonance	1135:1143	arg1	images					1145:1150	Magnetic resonance images	1126:1150	Magnetic resonance images (MRI)	1126:1156	Magnetic resonance images (MRI) were collected bilaterally and voxel by voxel T1ρ relaxation times were calculated using a five-image sequence and a monoexponential equation.
30560446	10	111	from	MFC	2320:2322	arg1	subsection					2173:2182	a subsection	2171:2182	a subsection of participants (n = 18) without a concomitant medial tibiofemoral compartment meniscal or chondral injury in the entire weight-bearing MFC, as well as anterior-MFC and central-MFC ROI of the ACLR and uninjured limb	2171:2398	A post hoc analysis found lesser strength and greater T1ρ relaxation times were significantly associated in a subsection of participants (n = 18) without a concomitant medial tibiofemoral compartment meniscal or chondral injury in the entire weight-bearing MFC, as well as anterior-MFC and central-MFC ROI of the ACLR and uninjured limb.
30560446	10	112	theme	lesser	2089:2094	arg1	strength					2096:2103	lesser strength	2089:2103	lesser strength	2089:2103	A post hoc analysis found lesser strength and greater T1ρ relaxation times were significantly associated in a subsection of participants (n = 18) without a concomitant medial tibiofemoral compartment meniscal or chondral injury in the entire weight-bearing MFC, as well as anterior-MFC and central-MFC ROI of the ACLR and uninjured limb.
30560446	7	113	theme	articular	1335:1343	arg1	cartilage					1345:1353	the articular cartilage	1331:1353	the articular cartilage for the weight-bearing surfaces of the medial and lateral femoral condyles (MFC and LFC)	1331:1442	Following image registration, the articular cartilage for the weight-bearing surfaces of the medial and lateral femoral condyles (MFC and LFC) were manually segmented and further sub-sectioned into posterior, central and anterior regions of interest (ROI) based on the corresponding meniscal anatomy viewed in the sagittal plane.
30560446	9	114	theme	RESULTS	1845:1851	arg1	strength					1871:1878	RESULTS Lesser quadriceps strength	1845:1878	RESULTS Lesser quadriceps strength	1845:1878	RESULTS Lesser quadriceps strength was significantly associated with greater T1ρ relaxation times in the entire weight-bearing MFC (R2 = 0.14, P = 0.05) and the anterior-MFC ROI (R2 = 0.22, P = 0.02) of the ACLR limb.
30560446	11	115	theme	extremity	2526:2534	arg1	strength					2543:2550	lower extremity muscle strength	2520:2550	lower extremity muscle strength	2520:2550	CONCLUSIONS The association between weaker quadriceps and greater T1ρ relaxation times in the MFC suggests deficits in lower extremity muscle strength may be related to cartilage composition as early as 6 months following ACLR.
30560446	7	116	dep	condyles	1421:1428	arg1	condyles					1421:1428	the medial and lateral femoral condyles	1390:1428	the medial and lateral femoral condyles (MFC and LFC)	1390:1442	Following image registration, the articular cartilage for the weight-bearing surfaces of the medial and lateral femoral condyles (MFC and LFC) were manually segmented and further sub-sectioned into posterior, central and anterior regions of interest (ROI) based on the corresponding meniscal anatomy viewed in the sagittal plane.
30560446	7	116	dep	condyles	1421:1428	arg1	LFC					1439:1441	LFC	1439:1441	LFC	1439:1441	Following image registration, the articular cartilage for the weight-bearing surfaces of the medial and lateral femoral condyles (MFC and LFC) were manually segmented and further sub-sectioned into posterior, central and anterior regions of interest (ROI) based on the corresponding meniscal anatomy viewed in the sagittal plane.
30560446	7	116	dep	condyles	1421:1428	arg1	MFC					1431:1433	MFC	1431:1433	MFC	1431:1433	Following image registration, the articular cartilage for the weight-bearing surfaces of the medial and lateral femoral condyles (MFC and LFC) were manually segmented and further sub-sectioned into posterior, central and anterior regions of interest (ROI) based on the corresponding meniscal anatomy viewed in the sagittal plane.
30560446	0	117	theme	femoral	78:84	arg1	cartilage					96:104	the medial femoral articular cartilage	67:104	the medial femoral articular cartilage 6 months following anterior cruciate ligament reconstruction	67:165	Quadriceps weakness associates with greater T1ρ relaxation time in the medial femoral articular cartilage 6 months following anterior cruciate ligament reconstruction.
30560446	1	118	theme	Quadriceps	176:185	arg1	weakness					187:194	PURPOSE Quadriceps weakness	168:194	PURPOSE Quadriceps weakness following anterior cruciate ligament reconstruction (ACLR)	168:253	PURPOSE Quadriceps weakness following anterior cruciate ligament reconstruction (ACLR) is linked to decreased patient-reported function, altered lower extremity biomechanics and tibiofemoral joint space narrowing.
30560446	8	119	from	strength	1725:1732	arg1	MFC					1788:1790	the entire weight-bearing MFC	1762:1790	the entire weight-bearing MFC	1762:1790	Univariate linear regression models were used to determine the association between quadriceps strength and T1ρ relaxation times in the entire weight-bearing MFC and LFC, as well as the ROI in each respective limb.
30560446	11	120	theme	T1ρ	2467:2469	arg1	times					2482:2486	greater T1ρ relaxation times	2459:2486	greater T1ρ relaxation times	2459:2486	CONCLUSIONS The association between weaker quadriceps and greater T1ρ relaxation times in the MFC suggests deficits in lower extremity muscle strength may be related to cartilage composition as early as 6 months following ACLR.
30560446	10	121	theme	T1ρ	2117:2119	arg1	times					2132:2136	greater T1ρ relaxation times	2109:2136	greater T1ρ relaxation times	2109:2136	A post hoc analysis found lesser strength and greater T1ρ relaxation times were significantly associated in a subsection of participants (n = 18) without a concomitant medial tibiofemoral compartment meniscal or chondral injury in the entire weight-bearing MFC, as well as anterior-MFC and central-MFC ROI of the ACLR and uninjured limb.
30560446	7	122	theme	image	1311:1315	arg1	registration					1317:1328	image registration	1311:1328	image registration	1311:1328	Following image registration, the articular cartilage for the weight-bearing surfaces of the medial and lateral femoral condyles (MFC and LFC) were manually segmented and further sub-sectioned into posterior, central and anterior regions of interest (ROI) based on the corresponding meniscal anatomy viewed in the sagittal plane.
30560446	0	123	from	time	59:62	arg1	6 months					106:113	the medial femoral articular cartilage 6 months	67:113	the medial femoral articular cartilage 6 months following anterior cruciate ligament reconstruction	67:165	Quadriceps weakness associates with greater T1ρ relaxation time in the medial femoral articular cartilage 6 months following anterior cruciate ligament reconstruction.
30560446	10	124	theme	weight-bearing	2305:2318	arg1	MFC					2320:2322	the entire weight-bearing MFC	2294:2322	the entire weight-bearing MFC	2294:2322	A post hoc analysis found lesser strength and greater T1ρ relaxation times were significantly associated in a subsection of participants (n = 18) without a concomitant medial tibiofemoral compartment meniscal or chondral injury in the entire weight-bearing MFC, as well as anterior-MFC and central-MFC ROI of the ACLR and uninjured limb.
30560446	5	125	theme	knee	1080:1083	arg1	flexion					1085:1091	90°of knee flexion	1074:1091	90°of knee flexion	1074:1091	METHODS Twenty-seven individuals (15 females, 12 males) with a patellar tendon autograft ACLR underwent isometric quadriceps strength assessments in 90°of knee flexion during a 6-month follow-up exam.
30560446	8	126	theme	linear	1642:1647	arg1	models					1660:1665	Univariate linear regression models	1631:1665	Univariate linear regression models	1631:1665	Univariate linear regression models were used to determine the association between quadriceps strength and T1ρ relaxation times in the entire weight-bearing MFC and LFC, as well as the ROI in each respective limb.
30560446	10	127	theme	anterior-MFC	2336:2347	arg1	subsection					2173:2182	a subsection	2171:2182	a subsection of participants (n = 18) without a concomitant medial tibiofemoral compartment meniscal or chondral injury in the entire weight-bearing MFC, as well as anterior-MFC and central-MFC ROI of the ACLR and uninjured limb	2171:2398	A post hoc analysis found lesser strength and greater T1ρ relaxation times were significantly associated in a subsection of participants (n = 18) without a concomitant medial tibiofemoral compartment meniscal or chondral injury in the entire weight-bearing MFC, as well as anterior-MFC and central-MFC ROI of the ACLR and uninjured limb.
30560446	2	128	theme	early	443:447	arg1	changes					461:467	early deleterious changes	443:467	early deleterious changes to femoral cartilage composition that are suggestive of posttraumatic osteoarthritis development	443:564	It remains unknown if quadriceps weakness is associated with early deleterious changes to femoral cartilage composition that are suggestive of posttraumatic osteoarthritis development.
30560446	11	129	from	deficits	2508:2515	arg1	strength					2543:2550	lower extremity muscle strength	2520:2550	lower extremity muscle strength	2520:2550	CONCLUSIONS The association between weaker quadriceps and greater T1ρ relaxation times in the MFC suggests deficits in lower extremity muscle strength may be related to cartilage composition as early as 6 months following ACLR.
30560446	5	130	theme	isometric	1029:1037	arg1	strength					1050:1057	isometric quadriceps strength	1029:1057	isometric quadriceps strength assessments in 90°of knee flexion	1029:1091	METHODS Twenty-seven individuals (15 females, 12 males) with a patellar tendon autograft ACLR underwent isometric quadriceps strength assessments in 90°of knee flexion during a 6-month follow-up exam.
30560446	9	131	theme	greater	1914:1920	arg1	times					1937:1941	greater T1ρ relaxation times	1914:1941	greater T1ρ relaxation times in the entire weight-bearing MFC (R2 = 0.14, P = 0.05) and the anterior-MFC ROI (R2 = 0.22, P = 0.02) of the ACLR limb	1914:2060	RESULTS Lesser quadriceps strength was significantly associated with greater T1ρ relaxation times in the entire weight-bearing MFC (R2 = 0.14, P = 0.05) and the anterior-MFC ROI (R2 = 0.22, P = 0.02) of the ACLR limb.
30560446	1	132	theme	extremity	319:327	arg1	biomechanics					329:340	altered lower extremity biomechanics	305:340	altered lower extremity biomechanics	305:340	PURPOSE Quadriceps weakness following anterior cruciate ligament reconstruction (ACLR) is linked to decreased patient-reported function, altered lower extremity biomechanics and tibiofemoral joint space narrowing.
30560446	11	133	theme	greater	2459:2465	arg1	times					2482:2486	greater T1ρ relaxation times	2459:2486	greater T1ρ relaxation times	2459:2486	CONCLUSIONS The association between weaker quadriceps and greater T1ρ relaxation times in the MFC suggests deficits in lower extremity muscle strength may be related to cartilage composition as early as 6 months following ACLR.
30560446	5	134	theme	strength	1050:1057	arg1	assessments					1059:1069	isometric quadriceps strength assessments	1029:1069	isometric quadriceps strength assessments in 90°of knee flexion	1029:1091	METHODS Twenty-seven individuals (15 females, 12 males) with a patellar tendon autograft ACLR underwent isometric quadriceps strength assessments in 90°of knee flexion during a 6-month follow-up exam.
30560446	7	135	theme	condyles	1421:1428	arg1	surfaces					1378:1385	the weight-bearing surfaces	1359:1385	the weight-bearing surfaces of the medial and lateral femoral condyles (MFC and LFC)	1359:1442	Following image registration, the articular cartilage for the weight-bearing surfaces of the medial and lateral femoral condyles (MFC and LFC) were manually segmented and further sub-sectioned into posterior, central and anterior regions of interest (ROI) based on the corresponding meniscal anatomy viewed in the sagittal plane.
30560446	9	136	theme	relaxation	1926:1935	arg1	times					1937:1941	greater T1ρ relaxation times	1914:1941	greater T1ρ relaxation times in the entire weight-bearing MFC (R2 = 0.14, P = 0.05) and the anterior-MFC ROI (R2 = 0.22, P = 0.02) of the ACLR limb	1914:2060	RESULTS Lesser quadriceps strength was significantly associated with greater T1ρ relaxation times in the entire weight-bearing MFC (R2 = 0.14, P = 0.05) and the anterior-MFC ROI (R2 = 0.22, P = 0.02) of the ACLR limb.
30560446	10	137	from	subsection	2173:2182	arg1	MFC					2320:2322	the entire weight-bearing MFC	2294:2322	the entire weight-bearing MFC	2294:2322	A post hoc analysis found lesser strength and greater T1ρ relaxation times were significantly associated in a subsection of participants (n = 18) without a concomitant medial tibiofemoral compartment meniscal or chondral injury in the entire weight-bearing MFC, as well as anterior-MFC and central-MFC ROI of the ACLR and uninjured limb.
30560446	3	138	from	times	683:687	arg1	condyles					785:792	the medial and lateral femoral condyles	754:792	the medial and lateral femoral condyles	754:792	The purpose of the cross-sectional study was to determine if quadriceps strength was associated with T1ρ relaxation times, a marker of proteoglycan density, of the articular cartilage in the medial and lateral femoral condyles 6 months following ACLR.
30560446	2	139	theme	femoral	472:478	arg1	composition					490:500	femoral cartilage composition	472:500	femoral cartilage composition	472:500	It remains unknown if quadriceps weakness is associated with early deleterious changes to femoral cartilage composition that are suggestive of posttraumatic osteoarthritis development.
30560446	0	140	theme	T1ρ	44:46	arg1	time					59:62	greater T1ρ relaxation time	36:62	greater T1ρ relaxation time in the medial femoral articular cartilage 6 months following anterior cruciate ligament reconstruction	36:165	Quadriceps weakness associates with greater T1ρ relaxation time in the medial femoral articular cartilage 6 months following anterior cruciate ligament reconstruction.
30560446	7	141	theme	lateral	1405:1411	arg1	condyles					1421:1428	the medial and lateral femoral condyles	1390:1428	the medial and lateral femoral condyles (MFC and LFC)	1390:1442	Following image registration, the articular cartilage for the weight-bearing surfaces of the medial and lateral femoral condyles (MFC and LFC) were manually segmented and further sub-sectioned into posterior, central and anterior regions of interest (ROI) based on the corresponding meniscal anatomy viewed in the sagittal plane.
30560446	7	141	theme	lateral	1405:1411	arg1	LFC					1439:1441	LFC	1439:1441	LFC	1439:1441	Following image registration, the articular cartilage for the weight-bearing surfaces of the medial and lateral femoral condyles (MFC and LFC) were manually segmented and further sub-sectioned into posterior, central and anterior regions of interest (ROI) based on the corresponding meniscal anatomy viewed in the sagittal plane.
30560446	7	141	theme	lateral	1405:1411	arg1	MFC					1431:1433	MFC	1431:1433	MFC	1431:1433	Following image registration, the articular cartilage for the weight-bearing surfaces of the medial and lateral femoral condyles (MFC and LFC) were manually segmented and further sub-sectioned into posterior, central and anterior regions of interest (ROI) based on the corresponding meniscal anatomy viewed in the sagittal plane.
30560446	2	142	theme	osteoarthritis	539:552	arg1	development					554:564	posttraumatic osteoarthritis development	525:564	posttraumatic osteoarthritis development	525:564	It remains unknown if quadriceps weakness is associated with early deleterious changes to femoral cartilage composition that are suggestive of posttraumatic osteoarthritis development.
30560446	5	143	with	individuals	946:956	arg1	ACLR					1014:1017	a patellar tendon autograft ACLR	986:1017	a patellar tendon autograft ACLR	986:1017	METHODS Twenty-seven individuals (15 females, 12 males) with a patellar tendon autograft ACLR underwent isometric quadriceps strength assessments in 90°of knee flexion during a 6-month follow-up exam.
30560446	3	144	theme	density	715:721	arg1	times					683:687	T1ρ relaxation times	668:687	T1ρ relaxation times	668:687	The purpose of the cross-sectional study was to determine if quadriceps strength was associated with T1ρ relaxation times, a marker of proteoglycan density, of the articular cartilage in the medial and lateral femoral condyles 6 months following ACLR.
30560446	3	144	theme	density	715:721	arg1	marker					692:697	a marker	690:697	a marker of proteoglycan density	690:721	The purpose of the cross-sectional study was to determine if quadriceps strength was associated with T1ρ relaxation times, a marker of proteoglycan density, of the articular cartilage in the medial and lateral femoral condyles 6 months following ACLR.
30560446	4	145	theme	proteoglycan	903:914	arg1	density					916:922	lesser proteoglycan density	896:922	lesser proteoglycan density	896:922	It is hypothesized that individuals with weaker quadriceps would demonstrate lesser proteoglycan density.
30560446	3	146	dep	6 months	794:801	arg1	following					803:811	following	803:811	following	803:811	The purpose of the cross-sectional study was to determine if quadriceps strength was associated with T1ρ relaxation times, a marker of proteoglycan density, of the articular cartilage in the medial and lateral femoral condyles 6 months following ACLR.
30560446	3	146	dep	6 months	794:801	arg1	ACLR					813:816	ACLR	813:816	ACLR	813:816	The purpose of the cross-sectional study was to determine if quadriceps strength was associated with T1ρ relaxation times, a marker of proteoglycan density, of the articular cartilage in the medial and lateral femoral condyles 6 months following ACLR.
30560446	7	147	theme	medial	1394:1399	arg1	condyles					1421:1428	the medial and lateral femoral condyles	1390:1428	the medial and lateral femoral condyles (MFC and LFC)	1390:1442	Following image registration, the articular cartilage for the weight-bearing surfaces of the medial and lateral femoral condyles (MFC and LFC) were manually segmented and further sub-sectioned into posterior, central and anterior regions of interest (ROI) based on the corresponding meniscal anatomy viewed in the sagittal plane.
30560446	7	147	theme	medial	1394:1399	arg1	LFC					1439:1441	LFC	1439:1441	LFC	1439:1441	Following image registration, the articular cartilage for the weight-bearing surfaces of the medial and lateral femoral condyles (MFC and LFC) were manually segmented and further sub-sectioned into posterior, central and anterior regions of interest (ROI) based on the corresponding meniscal anatomy viewed in the sagittal plane.
30560446	7	147	theme	medial	1394:1399	arg1	MFC					1431:1433	MFC	1431:1433	MFC	1431:1433	Following image registration, the articular cartilage for the weight-bearing surfaces of the medial and lateral femoral condyles (MFC and LFC) were manually segmented and further sub-sectioned into posterior, central and anterior regions of interest (ROI) based on the corresponding meniscal anatomy viewed in the sagittal plane.
30560446	9	148	theme	entire	1950:1955	arg1	P = 0.05					1988:1995	P = 0.05	1988:1995	P = 0.05	1988:1995	RESULTS Lesser quadriceps strength was significantly associated with greater T1ρ relaxation times in the entire weight-bearing MFC (R2 = 0.14, P = 0.05) and the anterior-MFC ROI (R2 = 0.22, P = 0.02) of the ACLR limb.
30560446	9	148	theme	entire	1950:1955	arg1	MFC					1972:1974	the entire weight-bearing MFC	1946:1974	the entire weight-bearing MFC (R2 = 0.14, P = 0.05)	1946:1996	RESULTS Lesser quadriceps strength was significantly associated with greater T1ρ relaxation times in the entire weight-bearing MFC (R2 = 0.14, P = 0.05) and the anterior-MFC ROI (R2 = 0.22, P = 0.02) of the ACLR limb.
30560446	8	149	theme	entire	1766:1771	arg1	MFC					1788:1790	the entire weight-bearing MFC	1762:1790	the entire weight-bearing MFC	1762:1790	Univariate linear regression models were used to determine the association between quadriceps strength and T1ρ relaxation times in the entire weight-bearing MFC and LFC, as well as the ROI in each respective limb.
30560446	7	150	theme	sagittal	1615:1622	arg1	plane					1624:1628	the sagittal plane	1611:1628	the sagittal plane	1611:1628	Following image registration, the articular cartilage for the weight-bearing surfaces of the medial and lateral femoral condyles (MFC and LFC) were manually segmented and further sub-sectioned into posterior, central and anterior regions of interest (ROI) based on the corresponding meniscal anatomy viewed in the sagittal plane.
30560446	8	151	theme	quadriceps	1714:1723	arg1	strength					1725:1732	quadriceps strength	1714:1732	quadriceps strength	1714:1732	Univariate linear regression models were used to determine the association between quadriceps strength and T1ρ relaxation times in the entire weight-bearing MFC and LFC, as well as the ROI in each respective limb.
30560446	3	152	theme	articular	731:739	arg1	cartilage					741:749	the articular cartilage	727:749	the articular cartilage in the medial and lateral femoral condyles 6 months following ACLR	727:816	The purpose of the cross-sectional study was to determine if quadriceps strength was associated with T1ρ relaxation times, a marker of proteoglycan density, of the articular cartilage in the medial and lateral femoral condyles 6 months following ACLR.
30560446	5	153	theme	6-month	1102:1108	arg1	follow-up					1110:1118	a 6-month follow-up	1100:1118	a 6-month follow-up exam	1100:1123	METHODS Twenty-seven individuals (15 females, 12 males) with a patellar tendon autograft ACLR underwent isometric quadriceps strength assessments in 90°of knee flexion during a 6-month follow-up exam.
30560446	7	154	theme	weight-bearing	1363:1376	arg1	surfaces					1378:1385	the weight-bearing surfaces	1359:1385	the weight-bearing surfaces of the medial and lateral femoral condyles (MFC and LFC)	1359:1442	Following image registration, the articular cartilage for the weight-bearing surfaces of the medial and lateral femoral condyles (MFC and LFC) were manually segmented and further sub-sectioned into posterior, central and anterior regions of interest (ROI) based on the corresponding meniscal anatomy viewed in the sagittal plane.
30560446	3	155	from	cartilage	741:749	arg1	condyles					785:792	the medial and lateral femoral condyles	754:792	the medial and lateral femoral condyles	754:792	The purpose of the cross-sectional study was to determine if quadriceps strength was associated with T1ρ relaxation times, a marker of proteoglycan density, of the articular cartilage in the medial and lateral femoral condyles 6 months following ACLR.
30560446	3	156	theme	lateral	769:775	arg1	condyles					785:792	the medial and lateral femoral condyles	754:792	the medial and lateral femoral condyles	754:792	The purpose of the cross-sectional study was to determine if quadriceps strength was associated with T1ρ relaxation times, a marker of proteoglycan density, of the articular cartilage in the medial and lateral femoral condyles 6 months following ACLR.
30560446	5	157	dep	individuals	946:956	arg1	females					962:968	15 females	959:968	15 females	959:968	METHODS Twenty-seven individuals (15 females, 12 males) with a patellar tendon autograft ACLR underwent isometric quadriceps strength assessments in 90°of knee flexion during a 6-month follow-up exam.
30560446	5	157	dep	individuals	946:956	arg1	males					974:978	12 males	971:978	12 males	971:978	METHODS Twenty-seven individuals (15 females, 12 males) with a patellar tendon autograft ACLR underwent isometric quadriceps strength assessments in 90°of knee flexion during a 6-month follow-up exam.
30560446	1	158	theme	decreased	268:276	arg1	function					295:302	decreased patient-reported function	268:302	decreased patient-reported function	268:302	PURPOSE Quadriceps weakness following anterior cruciate ligament reconstruction (ACLR) is linked to decreased patient-reported function, altered lower extremity biomechanics and tibiofemoral joint space narrowing.
31473407	7	0	theme	physical	1104:1111	arg1	properties					1113:1122	good physical properties	1099:1122	good physical properties	1099:1122	The results confirm that the PPEM membrane exhibits good physical properties with excellent antibacterial activity and successfully promotes periodontal tissue repair, making it promising for periodontal treatment.
31473407	1	1	theme	tooth-	165:170	arg1	tissues					183:189	tooth- supporting tissues	165:189	tooth- supporting tissues	165:189	Periodontitis is a chronic, destructive inflammatory disease that injures tooth- supporting tissues, eventually leading to tooth loss.
31473407	6	2	theme	membrane	975:982	arg1	functionality					949:961	the functionality	945:961	the functionality of the PPEM membrane	945:982	Additionally, the functionality of the PPEM membrane was evaluated in vivo with a periodontal defect model in rats.
31473407	6	3	from	model	1032:1036	arg1	rats					1041:1044	rats	1041:1044	rats	1041:1044	Additionally, the functionality of the PPEM membrane was evaluated in vivo with a periodontal defect model in rats.
31473407	1	4	theme	supporting	172:181	arg1	tissues					183:189	tooth- supporting tissues	165:189	tooth- supporting tissues	165:189	Periodontitis is a chronic, destructive inflammatory disease that injures tooth- supporting tissues, eventually leading to tooth loss.
31473407	5	5	theme	ultrastructural	796:810	arg1	analyses					812:819	Physical, morphological, and ultrastructural analyses	767:819	Physical, morphological, and ultrastructural analyses	767:819	Physical, morphological, and ultrastructural analyses were carried out in order to assess cellular compatibility, drug release and antibacterial activity in vitro.
31473407	3	6	theme	alveolar	628:635	arg1	repair					642:647	periodontal alveolar bone repair	616:647	periodontal alveolar bone repair	616:647	To address this challenge, we report a strategy for developing an enzyme-mediated periodontal membrane for targeted antibiotic delivery into infectious periodontal pockets; the unique components of the membrane will also benefit periodontal alveolar bone repair.
31473407	3	7	theme	bone	637:640	arg1	repair					642:647	periodontal alveolar bone repair	616:647	periodontal alveolar bone repair	616:647	To address this challenge, we report a strategy for developing an enzyme-mediated periodontal membrane for targeted antibiotic delivery into infectious periodontal pockets; the unique components of the membrane will also benefit periodontal alveolar bone repair.
31473407	4	8	theme	containing	688:697	arg1	polyphosphoester					699:714	a chitosan membrane containing polyphosphoester	668:714	a chitosan membrane containing polyphosphoester	668:714	In this approach, a chitosan membrane containing polyphosphoester and minocycline hydrochloride (PPEM) was prepared.
31473407	7	9	theme	periodontal	1239:1249	arg1	treatment					1251:1259	periodontal treatment	1239:1259	periodontal treatment	1239:1259	The results confirm that the PPEM membrane exhibits good physical properties with excellent antibacterial activity and successfully promotes periodontal tissue repair, making it promising for periodontal treatment.
31473407	2	10	theme	periodontal	312:322	arg1	healing					324:330	periodontal healing	312:330	periodontal healing	312:330	Complete eradication of periodontal pathogenic microorganisms is fundamental to allow periodontal healing and commonly precedes periodontal tissue regeneration.
31473407	6	11	theme	PPEM	970:973	arg1	membrane					975:982	the PPEM membrane	966:982	the PPEM membrane	966:982	Additionally, the functionality of the PPEM membrane was evaluated in vivo with a periodontal defect model in rats.
31473407	7	12	theme	periodontal	1188:1198	arg1	repair					1207:1212	periodontal tissue repair	1188:1212	periodontal tissue repair	1188:1212	The results confirm that the PPEM membrane exhibits good physical properties with excellent antibacterial activity and successfully promotes periodontal tissue repair, making it promising for periodontal treatment.
31473407	3	13	theme	infectious	528:537	arg1	pockets					551:557	infectious periodontal pockets	528:557	infectious periodontal pockets	528:557	To address this challenge, we report a strategy for developing an enzyme-mediated periodontal membrane for targeted antibiotic delivery into infectious periodontal pockets; the unique components of the membrane will also benefit periodontal alveolar bone repair.
31473407	3	14	theme	membrane	589:596	arg1	components					571:580	the unique components	560:580	the unique components of the membrane	560:596	To address this challenge, we report a strategy for developing an enzyme-mediated periodontal membrane for targeted antibiotic delivery into infectious periodontal pockets; the unique components of the membrane will also benefit periodontal alveolar bone repair.
31473407	5	15	theme	cellular	857:864	arg1	compatibility					866:878	cellular compatibility	857:878	cellular compatibility	857:878	Physical, morphological, and ultrastructural analyses were carried out in order to assess cellular compatibility, drug release and antibacterial activity in vitro.
31473407	7	16	theme	tissue	1200:1205	arg1	repair					1207:1212	periodontal tissue repair	1188:1212	periodontal tissue repair	1188:1212	The results confirm that the PPEM membrane exhibits good physical properties with excellent antibacterial activity and successfully promotes periodontal tissue repair, making it promising for periodontal treatment.
31473407	3	17	theme	targeted	494:501	arg1	delivery					514:521	targeted antibiotic delivery	494:521	targeted antibiotic delivery into infectious periodontal pockets	494:557	To address this challenge, we report a strategy for developing an enzyme-mediated periodontal membrane for targeted antibiotic delivery into infectious periodontal pockets; the unique components of the membrane will also benefit periodontal alveolar bone repair.
31473407	0	18	theme	enzyme-responsive	3:19	arg1	membrane					21:28	An enzyme-responsive membrane	0:28	An enzyme-responsive membrane for antibiotic drug release and local periodontal treatment	0:88	An enzyme-responsive membrane for antibiotic drug release and local periodontal treatment.
31473407	2	19	theme	periodontal	354:364	arg1	regeneration					373:384	periodontal tissue regeneration	354:384	periodontal tissue regeneration	354:384	Complete eradication of periodontal pathogenic microorganisms is fundamental to allow periodontal healing and commonly precedes periodontal tissue regeneration.
31473407	3	20	theme	periodontal	469:479	arg1	membrane					481:488	an enzyme-mediated periodontal membrane	450:488	an enzyme-mediated periodontal membrane for targeted antibiotic delivery into infectious periodontal pockets	450:557	To address this challenge, we report a strategy for developing an enzyme-mediated periodontal membrane for targeted antibiotic delivery into infectious periodontal pockets; the unique components of the membrane will also benefit periodontal alveolar bone repair.
31473407	3	21	theme	periodontal	616:626	arg1	repair					642:647	periodontal alveolar bone repair	616:647	periodontal alveolar bone repair	616:647	To address this challenge, we report a strategy for developing an enzyme-mediated periodontal membrane for targeted antibiotic delivery into infectious periodontal pockets; the unique components of the membrane will also benefit periodontal alveolar bone repair.
31473407	5	22	theme	antibacterial	898:910	arg1	activity					912:919	antibacterial activity	898:919	antibacterial activity	898:919	Physical, morphological, and ultrastructural analyses were carried out in order to assess cellular compatibility, drug release and antibacterial activity in vitro.
31473407	5	23	theme	morphological	777:789	arg1	analyses					812:819	Physical, morphological, and ultrastructural analyses	767:819	Physical, morphological, and ultrastructural analyses	767:819	Physical, morphological, and ultrastructural analyses were carried out in order to assess cellular compatibility, drug release and antibacterial activity in vitro.
31473407	1	24	theme	chronic	110:116	arg1	disease					144:150	a chronic, destructive inflammatory disease	108:150	disease	144:150	Periodontitis is a chronic, destructive inflammatory disease that injures tooth- supporting tissues, eventually leading to tooth loss.
31473407	1	24	theme	chronic	110:116	arg1	Periodontitis					91:103	Periodontitis	91:103	Periodontitis	91:103	Periodontitis is a chronic, destructive inflammatory disease that injures tooth- supporting tissues, eventually leading to tooth loss.
31473407	1	25	theme	tooth	214:218	arg1	loss					220:223	tooth loss	214:223	tooth loss	214:223	Periodontitis is a chronic, destructive inflammatory disease that injures tooth- supporting tissues, eventually leading to tooth loss.
31473407	0	26	theme	drug	45:48	arg1	release					50:56	antibiotic drug release	34:56	antibiotic drug release	34:56	An enzyme-responsive membrane for antibiotic drug release and local periodontal treatment.
31473407	7	27	theme	excellent	1129:1137	arg1	activity					1153:1160	excellent antibacterial activity	1129:1160	excellent antibacterial activity	1129:1160	The results confirm that the PPEM membrane exhibits good physical properties with excellent antibacterial activity and successfully promotes periodontal tissue repair, making it promising for periodontal treatment.
31473407	4	28	theme	minocycline	720:730	arg1	PPEM					747:750	PPEM	747:750	PPEM	747:750	In this approach, a chitosan membrane containing polyphosphoester and minocycline hydrochloride (PPEM) was prepared.
31473407	4	28	theme	minocycline	720:730	arg1	hydrochloride					732:744	minocycline hydrochloride	720:744	minocycline hydrochloride (PPEM)	720:751	In this approach, a chitosan membrane containing polyphosphoester and minocycline hydrochloride (PPEM) was prepared.
31473407	2	29	theme	periodontal	250:260	arg1	microorganisms					273:286	periodontal pathogenic microorganisms	250:286	periodontal pathogenic microorganisms	250:286	Complete eradication of periodontal pathogenic microorganisms is fundamental to allow periodontal healing and commonly precedes periodontal tissue regeneration.
31473407	3	30	theme	antibiotic	503:512	arg1	delivery					514:521	targeted antibiotic delivery	494:521	targeted antibiotic delivery into infectious periodontal pockets	494:557	To address this challenge, we report a strategy for developing an enzyme-mediated periodontal membrane for targeted antibiotic delivery into infectious periodontal pockets; the unique components of the membrane will also benefit periodontal alveolar bone repair.
31473407	0	31	theme	antibiotic	34:43	arg1	release					50:56	antibiotic drug release	34:56	antibiotic drug release	34:56	An enzyme-responsive membrane for antibiotic drug release and local periodontal treatment.
31473407	6	32	theme	defect	1025:1030	arg1	model					1032:1036	a periodontal defect model	1011:1036	a periodontal defect model in rats	1011:1044	Additionally, the functionality of the PPEM membrane was evaluated in vivo with a periodontal defect model in rats.
31473407	3	33	theme	enzyme-mediated	453:467	arg1	membrane					481:488	an enzyme-mediated periodontal membrane	450:488	an enzyme-mediated periodontal membrane for targeted antibiotic delivery into infectious periodontal pockets	450:557	To address this challenge, we report a strategy for developing an enzyme-mediated periodontal membrane for targeted antibiotic delivery into infectious periodontal pockets; the unique components of the membrane will also benefit periodontal alveolar bone repair.
31473407	3	34	theme	unique	564:569	arg1	components					571:580	the unique components	560:580	the unique components of the membrane	560:596	To address this challenge, we report a strategy for developing an enzyme-mediated periodontal membrane for targeted antibiotic delivery into infectious periodontal pockets; the unique components of the membrane will also benefit periodontal alveolar bone repair.
31473407	7	35	theme	antibacterial	1139:1151	arg1	activity					1153:1160	excellent antibacterial activity	1129:1160	excellent antibacterial activity	1129:1160	The results confirm that the PPEM membrane exhibits good physical properties with excellent antibacterial activity and successfully promotes periodontal tissue repair, making it promising for periodontal treatment.
31473407	5	36	dep	carried	826:832	arg1	out					834:836	out	834:836	out	834:836	Physical, morphological, and ultrastructural analyses were carried out in order to assess cellular compatibility, drug release and antibacterial activity in vitro.
31473407	2	37	theme	Complete	226:233	arg1	eradication					235:245	Complete eradication	226:245	Complete eradication of periodontal pathogenic microorganisms	226:286	Complete eradication of periodontal pathogenic microorganisms is fundamental to allow periodontal healing and commonly precedes periodontal tissue regeneration.
31473407	1	38	theme	destructive	119:129	arg1	disease					144:150	a chronic, destructive inflammatory disease	108:150	disease	144:150	Periodontitis is a chronic, destructive inflammatory disease that injures tooth- supporting tissues, eventually leading to tooth loss.
31473407	1	38	theme	destructive	119:129	arg1	Periodontitis					91:103	Periodontitis	91:103	Periodontitis	91:103	Periodontitis is a chronic, destructive inflammatory disease that injures tooth- supporting tissues, eventually leading to tooth loss.
31473407	7	39	theme	good	1099:1102	arg1	properties					1113:1122	good physical properties	1099:1122	good physical properties	1099:1122	The results confirm that the PPEM membrane exhibits good physical properties with excellent antibacterial activity and successfully promotes periodontal tissue repair, making it promising for periodontal treatment.
31473407	7	40	theme	PPEM	1076:1079	arg1	membrane					1081:1088	the PPEM membrane	1072:1088	the PPEM membrane	1072:1088	The results confirm that the PPEM membrane exhibits good physical properties with excellent antibacterial activity and successfully promotes periodontal tissue repair, making it promising for periodontal treatment.
31473407	1	41	theme	inflammatory	131:142	arg1	disease					144:150	a chronic, destructive inflammatory disease	108:150	disease	144:150	Periodontitis is a chronic, destructive inflammatory disease that injures tooth- supporting tissues, eventually leading to tooth loss.
31473407	1	41	theme	inflammatory	131:142	arg1	Periodontitis					91:103	Periodontitis	91:103	Periodontitis	91:103	Periodontitis is a chronic, destructive inflammatory disease that injures tooth- supporting tissues, eventually leading to tooth loss.
31473407	5	42	theme	Physical	767:774	arg1	analyses					812:819	Physical, morphological, and ultrastructural analyses	767:819	Physical, morphological, and ultrastructural analyses	767:819	Physical, morphological, and ultrastructural analyses were carried out in order to assess cellular compatibility, drug release and antibacterial activity in vitro.
31473407	2	43	theme	tissue	366:371	arg1	regeneration					373:384	periodontal tissue regeneration	354:384	periodontal tissue regeneration	354:384	Complete eradication of periodontal pathogenic microorganisms is fundamental to allow periodontal healing and commonly precedes periodontal tissue regeneration.
31473407	3	44	theme	periodontal	539:549	arg1	pockets					551:557	infectious periodontal pockets	528:557	infectious periodontal pockets	528:557	To address this challenge, we report a strategy for developing an enzyme-mediated periodontal membrane for targeted antibiotic delivery into infectious periodontal pockets; the unique components of the membrane will also benefit periodontal alveolar bone repair.
31473407	0	45	theme	periodontal	68:78	arg1	treatment					80:88	local periodontal treatment	62:88	local periodontal treatment	62:88	An enzyme-responsive membrane for antibiotic drug release and local periodontal treatment.
31473407	2	46	theme	microorganisms	273:286	arg1	eradication					235:245	Complete eradication	226:245	Complete eradication of periodontal pathogenic microorganisms	226:286	Complete eradication of periodontal pathogenic microorganisms is fundamental to allow periodontal healing and commonly precedes periodontal tissue regeneration.
31473407	4	47	theme	membrane	679:686	arg1	polyphosphoester					699:714	a chitosan membrane containing polyphosphoester	668:714	a chitosan membrane containing polyphosphoester	668:714	In this approach, a chitosan membrane containing polyphosphoester and minocycline hydrochloride (PPEM) was prepared.
31473407	0	48	theme	local	62:66	arg1	treatment					80:88	local periodontal treatment	62:88	local periodontal treatment	62:88	An enzyme-responsive membrane for antibiotic drug release and local periodontal treatment.
31473407	2	49	theme	pathogenic	262:271	arg1	microorganisms					273:286	periodontal pathogenic microorganisms	250:286	periodontal pathogenic microorganisms	250:286	Complete eradication of periodontal pathogenic microorganisms is fundamental to allow periodontal healing and commonly precedes periodontal tissue regeneration.
31473407	5	50	theme	drug	881:884	arg1	release					886:892	drug release	881:892	drug release	881:892	Physical, morphological, and ultrastructural analyses were carried out in order to assess cellular compatibility, drug release and antibacterial activity in vitro.
31473407	6	51	theme	periodontal	1013:1023	arg1	model					1032:1036	a periodontal defect model	1011:1036	a periodontal defect model in rats	1011:1044	Additionally, the functionality of the PPEM membrane was evaluated in vivo with a periodontal defect model in rats.
31473407	4	52	theme	chitosan	670:677	arg1	polyphosphoester					699:714	a chitosan membrane containing polyphosphoester	668:714	a chitosan membrane containing polyphosphoester	668:714	In this approach, a chitosan membrane containing polyphosphoester and minocycline hydrochloride (PPEM) was prepared.
30154681	9	0	dep	aegypti	1144:1150	arg1	whereas					1183:1189	whereas	1183:1189	whereas	1183:1189	aegypti with high percentage for 27 d, whereas free O. gratissimum oil showed a short period of time (8 d) in this activity.
30154681	3	1	theme	Chemical	404:411	arg1	analysis					413:420	Chemical analysis	404:420	Chemical analysis	404:420	Chemical analysis of O. gratissimum oil obtained by hydrodistillation, using gas chromatography-mass spectroscopy techniques, presented eugenol (67.38%) and Z-β-ocimene (14.95 %) as major constituents.
30154681	10	2	theme	storage	1349:1355	arg1	condition					1357:1365	storage condition	1349:1365	storage condition	1349:1365	The stability study showed the stability of oil content and its compositions in storage condition.
30154681	9	3	theme	high	1157:1160	arg1	percentage					1162:1171	high percentage	1157:1171	high percentage	1157:1171	aegypti with high percentage for 27 d, whereas free O. gratissimum oil showed a short period of time (8 d) in this activity.
30154681	5	4	theme	good	803:806	arg1	hardness					808:815	good hardness	803:815	good hardness	803:815	The beads exhibited a good spherical shape and good hardness and flexibility with an average size of 1.49 ± 1.36 mm.
30154681	1	5	dep	aegypti	370:376	arg1	Ae					379:380	Ae	379:380	Ae	379:380	This study was aimed at investigating the oviposition deterrent activity of Ocimum gratissimum (L.) essential oil (O. gratissimum oil) and its product, Ocimum gratissimum (L.)- alginate beads (beads), against Aedes aegypti (Ae.
30154681	3	6	theme	major	586:590	arg1	constituents					592:603	major constituents	586:603	major constituents	586:603	Chemical analysis of O. gratissimum oil obtained by hydrodistillation, using gas chromatography-mass spectroscopy techniques, presented eugenol (67.38%) and Z-β-ocimene (14.95 %) as major constituents.
30154681	3	6	theme	major	586:590	arg1	eugenol					540:546	eugenol	540:546	eugenol (67.38%)	540:555	Chemical analysis of O. gratissimum oil obtained by hydrodistillation, using gas chromatography-mass spectroscopy techniques, presented eugenol (67.38%) and Z-β-ocimene (14.95 %) as major constituents.
30154681	3	6	theme	major	586:590	arg1	Z-β-ocimene					561:571	Z-β-ocimene	561:571	Z-β-ocimene (14.95 %)	561:581	Chemical analysis of O. gratissimum oil obtained by hydrodistillation, using gas chromatography-mass spectroscopy techniques, presented eugenol (67.38%) and Z-β-ocimene (14.95 %) as major constituents.
30154681	4	7	theme	gelation	671:678	arg1	method					680:685	the orifice-ionic gelation method	653:685	the orifice-ionic gelation method with calcium chloride as hardening agent and Tween®20 as emulsifier	653:753	Good characteristics of beads were obtained by the orifice-ionic gelation method with calcium chloride as hardening agent and Tween®20 as emulsifier.
30154681	1	8	theme	Ocimum	231:236	arg1	oil					285:287	O. gratissimum oil	270:287	O. gratissimum oil	270:287	This study was aimed at investigating the oviposition deterrent activity of Ocimum gratissimum (L.) essential oil (O. gratissimum oil) and its product, Ocimum gratissimum (L.)- alginate beads (beads), against Aedes aegypti (Ae.
30154681	1	8	theme	Ocimum	231:236	arg1	oil					265:267	Ocimum gratissimum (L.) essential oil	231:267	Ocimum gratissimum (L.) essential oil (O. gratissimum oil)	231:288	This study was aimed at investigating the oviposition deterrent activity of Ocimum gratissimum (L.) essential oil (O. gratissimum oil) and its product, Ocimum gratissimum (L.)- alginate beads (beads), against Aedes aegypti (Ae.
30154681	0	9	theme	gratissimum	90:100	arg1	Beads					120:124	Ocimum gratissimum (L.) Oil-Alginate Beads	83:124	Ocimum gratissimum (L.) Oil-Alginate Beads	83:124	Oviposition Deterrent Efficacy and Characteristics of a Botanical Natural Product, Ocimum gratissimum (L.) Oil-Alginate Beads, against Aedes aegypti (L.).
30154681	0	9	theme	gratissimum	90:100	arg1	Product					74:80	a Botanical Natural Product	54:80	a Botanical Natural Product	54:80	Oviposition Deterrent Efficacy and Characteristics of a Botanical Natural Product, Ocimum gratissimum (L.) Oil-Alginate Beads, against Aedes aegypti (L.).
30154681	1	10	theme	product	298:304	arg1	activity					219:226	the oviposition deterrent activity	193:226	the oviposition deterrent activity of Ocimum gratissimum (L.) essential oil (O. gratissimum oil) and its product, Ocimum gratissimum (L.)- alginate beads (beads), against Aedes aegypti (Ae	193:380	This study was aimed at investigating the oviposition deterrent activity of Ocimum gratissimum (L.) essential oil (O. gratissimum oil) and its product, Ocimum gratissimum (L.)- alginate beads (beads), against Aedes aegypti (Ae.
30154681	4	11	theme	orifice-ionic	657:669	arg1	method					680:685	the orifice-ionic gelation method	653:685	the orifice-ionic gelation method with calcium chloride as hardening agent and Tween®20 as emulsifier	653:753	Good characteristics of beads were obtained by the orifice-ionic gelation method with calcium chloride as hardening agent and Tween®20 as emulsifier.
30154681	1	12	theme	gratissimum	238:248	arg1	oil					285:287	O. gratissimum oil	270:287	O. gratissimum oil	270:287	This study was aimed at investigating the oviposition deterrent activity of Ocimum gratissimum (L.) essential oil (O. gratissimum oil) and its product, Ocimum gratissimum (L.)- alginate beads (beads), against Aedes aegypti (Ae.
30154681	1	12	theme	gratissimum	238:248	arg1	oil					265:267	Ocimum gratissimum (L.) essential oil	231:267	Ocimum gratissimum (L.) essential oil (O. gratissimum oil)	231:288	This study was aimed at investigating the oviposition deterrent activity of Ocimum gratissimum (L.) essential oil (O. gratissimum oil) and its product, Ocimum gratissimum (L.)- alginate beads (beads), against Aedes aegypti (Ae.
30154681	7	13	theme	10th	1045:1048	arg1	d					1050:1050	the 10th d	1041:1050	the 10th d	1041:1050	The beads (formulation code, F2) could prolong the essential oil release until the 10th d.
30154681	9	14	theme	time	1240:1243	arg1	period					1230:1235	a short period	1222:1235	a short period of time (8 d) in this activity	1222:1266	aegypti with high percentage for 27 d, whereas free O. gratissimum oil showed a short period of time (8 d) in this activity.
30154681	3	15	theme	gratissimum	428:438	arg1	oil					440:442	O. gratissimum oil	425:442	O. gratissimum oil obtained by hydrodistillation	425:472	Chemical analysis of O. gratissimum oil obtained by hydrodistillation, using gas chromatography-mass spectroscopy techniques, presented eugenol (67.38%) and Z-β-ocimene (14.95 %) as major constituents.
30154681	8	16	theme	oviposition	1093:1103	arg1	activity					1116:1123	a remarkably longer oviposition deterrence activity	1073:1123	a remarkably longer oviposition deterrence activity against gravid Ae	1073:1141	This beads provided a remarkably longer oviposition deterrence activity against gravid Ae.
30154681	0	17	dep	gratissimum	90:100	arg1	L.					103:104	L.	103:104	L.	103:104	Oviposition Deterrent Efficacy and Characteristics of a Botanical Natural Product, Ocimum gratissimum (L.) Oil-Alginate Beads, against Aedes aegypti (L.).
30154681	8	18	theme	longer	1086:1091	arg1	activity					1116:1123	a remarkably longer oviposition deterrence activity	1073:1123	a remarkably longer oviposition deterrence activity against gravid Ae	1073:1141	This beads provided a remarkably longer oviposition deterrence activity against gravid Ae.
30154681	9	19	theme	gratissimum	1199:1209	arg1	oil					1211:1213	free O. gratissimum oil	1191:1213	free O. gratissimum oil	1191:1213	aegypti with high percentage for 27 d, whereas free O. gratissimum oil showed a short period of time (8 d) in this activity.
30154681	11	20	theme	affordable	1391:1400	arg1	approaches					1402:1411	very affordable approaches	1386:1411	very affordable approaches	1386:1411	These results are very affordable approaches to control the dengue fever.
30154681	11	20	theme	affordable	1391:1400	arg1	results					1374:1380	These results	1368:1380	These results	1368:1380	These results are very affordable approaches to control the dengue fever.
30154681	5	21	theme	average	841:847	arg1	size					849:852	an average size	838:852	an average size of 1.49 ± 1.36 mm	838:870	The beads exhibited a good spherical shape and good hardness and flexibility with an average size of 1.49 ± 1.36 mm.
30154681	5	22	theme	good	778:781	arg1	shape					793:797	a good spherical shape	776:797	a good spherical shape	776:797	The beads exhibited a good spherical shape and good hardness and flexibility with an average size of 1.49 ± 1.36 mm.
30154681	9	23	theme	free	1191:1194	arg1	oil					1211:1213	free O. gratissimum oil	1191:1213	free O. gratissimum oil	1191:1213	aegypti with high percentage for 27 d, whereas free O. gratissimum oil showed a short period of time (8 d) in this activity.
30154681	10	24	theme	stability	1273:1281	arg1	study					1283:1287	The stability study	1269:1287	The stability study	1269:1287	The stability study showed the stability of oil content and its compositions in storage condition.
30154681	3	25	theme	gas	481:483	arg1	spectroscopy					505:516	gas chromatography-mass spectroscopy	481:516	gas chromatography-mass spectroscopy techniques	481:527	Chemical analysis of O. gratissimum oil obtained by hydrodistillation, using gas chromatography-mass spectroscopy techniques, presented eugenol (67.38%) and Z-β-ocimene (14.95 %) as major constituents.
30154681	5	26	theme	spherical	783:791	arg1	shape					793:797	a good spherical shape	776:797	a good spherical shape	776:797	The beads exhibited a good spherical shape and good hardness and flexibility with an average size of 1.49 ± 1.36 mm.
30154681	7	27	theme	oil	1023:1025	arg1	release					1027:1033	the essential oil release	1009:1033	the essential oil release	1009:1033	The beads (formulation code, F2) could prolong the essential oil release until the 10th d.
30154681	1	28	theme	L.	251:252	arg1	oil					285:287	O. gratissimum oil	270:287	O. gratissimum oil	270:287	This study was aimed at investigating the oviposition deterrent activity of Ocimum gratissimum (L.) essential oil (O. gratissimum oil) and its product, Ocimum gratissimum (L.)- alginate beads (beads), against Aedes aegypti (Ae.
30154681	1	28	theme	L.	251:252	arg1	oil					265:267	Ocimum gratissimum (L.) essential oil	231:267	Ocimum gratissimum (L.) essential oil (O. gratissimum oil)	231:288	This study was aimed at investigating the oviposition deterrent activity of Ocimum gratissimum (L.) essential oil (O. gratissimum oil) and its product, Ocimum gratissimum (L.)- alginate beads (beads), against Aedes aegypti (Ae.
30154681	10	29	theme	content	1317:1323	arg1	stability					1300:1308	the stability	1296:1308	the stability of oil content and its compositions in storage condition	1296:1365	The stability study showed the stability of oil content and its compositions in storage condition.
30154681	8	30	theme	gravid	1133:1138	arg1	Ae					1140:1141	gravid Ae	1133:1141	gravid Ae	1133:1141	This beads provided a remarkably longer oviposition deterrence activity against gravid Ae.
30154681	9	31	theme	O.	1196:1197	arg1	oil					1211:1213	free O. gratissimum oil	1191:1213	free O. gratissimum oil	1191:1213	aegypti with high percentage for 27 d, whereas free O. gratissimum oil showed a short period of time (8 d) in this activity.
30154681	0	32	theme	Oil-Alginate	107:118	arg1	Beads					120:124	Ocimum gratissimum (L.) Oil-Alginate Beads	83:124	Ocimum gratissimum (L.) Oil-Alginate Beads	83:124	Oviposition Deterrent Efficacy and Characteristics of a Botanical Natural Product, Ocimum gratissimum (L.) Oil-Alginate Beads, against Aedes aegypti (L.).
30154681	0	32	theme	Oil-Alginate	107:118	arg1	Product					74:80	a Botanical Natural Product	54:80	a Botanical Natural Product	54:80	Oviposition Deterrent Efficacy and Characteristics of a Botanical Natural Product, Ocimum gratissimum (L.) Oil-Alginate Beads, against Aedes aegypti (L.).
30154681	6	33	theme	entrapping	920:929	arg1	efficiency					931:940	the entrapping efficiency	916:940	the entrapping efficiency	916:940	The oil content, the yield percentage, and the entrapping efficiency were also examined.
30154681	1	34	theme	essential	255:263	arg1	oil					285:287	O. gratissimum oil	270:287	O. gratissimum oil	270:287	This study was aimed at investigating the oviposition deterrent activity of Ocimum gratissimum (L.) essential oil (O. gratissimum oil) and its product, Ocimum gratissimum (L.)- alginate beads (beads), against Aedes aegypti (Ae.
30154681	1	34	theme	essential	255:263	arg1	oil					265:267	Ocimum gratissimum (L.) essential oil	231:267	Ocimum gratissimum (L.) essential oil (O. gratissimum oil)	231:288	This study was aimed at investigating the oviposition deterrent activity of Ocimum gratissimum (L.) essential oil (O. gratissimum oil) and its product, Ocimum gratissimum (L.)- alginate beads (beads), against Aedes aegypti (Ae.
30154681	9	35	with	aegypti	1144:1150	arg1	percentage					1162:1171	high percentage	1157:1171	high percentage	1157:1171	aegypti with high percentage for 27 d, whereas free O. gratissimum oil showed a short period of time (8 d) in this activity.
30154681	8	36	theme	deterrence	1105:1114	arg1	activity					1116:1123	a remarkably longer oviposition deterrence activity	1073:1123	a remarkably longer oviposition deterrence activity against gravid Ae	1073:1141	This beads provided a remarkably longer oviposition deterrence activity against gravid Ae.
30154681	7	37	theme	essential	1013:1021	arg1	release					1027:1033	the essential oil release	1009:1033	the essential oil release	1009:1033	The beads (formulation code, F2) could prolong the essential oil release until the 10th d.
30154681	1	38	theme	oil	265:267	arg1	activity					219:226	the oviposition deterrent activity	193:226	the oviposition deterrent activity of Ocimum gratissimum (L.) essential oil (O. gratissimum oil) and its product, Ocimum gratissimum (L.)- alginate beads (beads), against Aedes aegypti (Ae	193:380	This study was aimed at investigating the oviposition deterrent activity of Ocimum gratissimum (L.) essential oil (O. gratissimum oil) and its product, Ocimum gratissimum (L.)- alginate beads (beads), against Aedes aegypti (Ae.
30154681	7	39	dep	beads	966:970	arg1	formulation					973:983	formulation	973:983	formulation	973:983	The beads (formulation code, F2) could prolong the essential oil release until the 10th d.
30154681	7	39	dep	beads	966:970	arg1	F2					991:992	F2	991:992	F2	991:992	The beads (formulation code, F2) could prolong the essential oil release until the 10th d.
30154681	6	40	theme	yield	894:898	arg1	percentage					900:909	the yield percentage	890:909	the yield percentage	890:909	The oil content, the yield percentage, and the entrapping efficiency were also examined.
30154681	9	41	theme	short	1224:1228	arg1	period					1230:1235	a short period	1222:1235	a short period of time (8 d) in this activity	1222:1266	aegypti with high percentage for 27 d, whereas free O. gratissimum oil showed a short period of time (8 d) in this activity.
30154681	0	42	theme	Botanical	56:64	arg1	Product					74:80	a Botanical Natural Product	54:80	a Botanical Natural Product	54:80	Oviposition Deterrent Efficacy and Characteristics of a Botanical Natural Product, Ocimum gratissimum (L.) Oil-Alginate Beads, against Aedes aegypti (L.).
30154681	0	42	theme	Botanical	56:64	arg1	Beads					120:124	Ocimum gratissimum (L.) Oil-Alginate Beads	83:124	Ocimum gratissimum (L.) Oil-Alginate Beads	83:124	Oviposition Deterrent Efficacy and Characteristics of a Botanical Natural Product, Ocimum gratissimum (L.) Oil-Alginate Beads, against Aedes aegypti (L.).
30154681	4	43	with	method	680:685	arg1	chloride					700:707	calcium chloride	692:707	calcium chloride as hardening agent and Tween®20 as emulsifier	692:753	Good characteristics of beads were obtained by the orifice-ionic gelation method with calcium chloride as hardening agent and Tween®20 as emulsifier.
30154681	3	44	theme	chromatography-mass	485:503	arg1	spectroscopy					505:516	gas chromatography-mass spectroscopy	481:516	gas chromatography-mass spectroscopy techniques	481:527	Chemical analysis of O. gratissimum oil obtained by hydrodistillation, using gas chromatography-mass spectroscopy techniques, presented eugenol (67.38%) and Z-β-ocimene (14.95 %) as major constituents.
30154681	7	45	dep	formulation	973:983	arg1	code					985:988	code	985:988	code	985:988	The beads (formulation code, F2) could prolong the essential oil release until the 10th d.
30154681	1	46	theme	O.	270:271	arg1	oil					285:287	O. gratissimum oil	270:287	O. gratissimum oil	270:287	This study was aimed at investigating the oviposition deterrent activity of Ocimum gratissimum (L.) essential oil (O. gratissimum oil) and its product, Ocimum gratissimum (L.)- alginate beads (beads), against Aedes aegypti (Ae.
30154681	1	46	theme	O.	270:271	arg1	oil					265:267	Ocimum gratissimum (L.) essential oil	231:267	Ocimum gratissimum (L.) essential oil (O. gratissimum oil)	231:288	This study was aimed at investigating the oviposition deterrent activity of Ocimum gratissimum (L.) essential oil (O. gratissimum oil) and its product, Ocimum gratissimum (L.)- alginate beads (beads), against Aedes aegypti (Ae.
30154681	10	47	theme	compositions	1333:1344	arg1	stability					1300:1308	the stability	1296:1308	the stability of oil content and its compositions in storage condition	1296:1365	The stability study showed the stability of oil content and its compositions in storage condition.
30154681	1	48	theme	alginate	332:339	arg1	beads					341:345	alginate beads	332:345	alginate beads (beads)	332:353	This study was aimed at investigating the oviposition deterrent activity of Ocimum gratissimum (L.) essential oil (O. gratissimum oil) and its product, Ocimum gratissimum (L.)- alginate beads (beads), against Aedes aegypti (Ae.
30154681	1	48	theme	alginate	332:339	arg1	product					298:304	its product	294:304	its product	294:304	This study was aimed at investigating the oviposition deterrent activity of Ocimum gratissimum (L.) essential oil (O. gratissimum oil) and its product, Ocimum gratissimum (L.)- alginate beads (beads), against Aedes aegypti (Ae.
30154681	1	48	theme	alginate	332:339	arg1	beads					348:352	beads	348:352	beads	348:352	This study was aimed at investigating the oviposition deterrent activity of Ocimum gratissimum (L.) essential oil (O. gratissimum oil) and its product, Ocimum gratissimum (L.)- alginate beads (beads), against Aedes aegypti (Ae.
30154681	3	49	theme	spectroscopy	505:516	arg1	techniques					518:527	gas chromatography-mass spectroscopy techniques	481:527	gas chromatography-mass spectroscopy techniques	481:527	Chemical analysis of O. gratissimum oil obtained by hydrodistillation, using gas chromatography-mass spectroscopy techniques, presented eugenol (67.38%) and Z-β-ocimene (14.95 %) as major constituents.
30154681	4	50	theme	Good	606:609	arg1	characteristics					611:625	Good characteristics	606:625	Good characteristics of beads	606:634	Good characteristics of beads were obtained by the orifice-ionic gelation method with calcium chloride as hardening agent and Tween®20 as emulsifier.
30154681	1	51	theme	gratissimum	273:283	arg1	oil					285:287	O. gratissimum oil	270:287	O. gratissimum oil	270:287	This study was aimed at investigating the oviposition deterrent activity of Ocimum gratissimum (L.) essential oil (O. gratissimum oil) and its product, Ocimum gratissimum (L.)- alginate beads (beads), against Aedes aegypti (Ae.
30154681	1	51	theme	gratissimum	273:283	arg1	oil					265:267	Ocimum gratissimum (L.) essential oil	231:267	Ocimum gratissimum (L.) essential oil (O. gratissimum oil)	231:288	This study was aimed at investigating the oviposition deterrent activity of Ocimum gratissimum (L.) essential oil (O. gratissimum oil) and its product, Ocimum gratissimum (L.)- alginate beads (beads), against Aedes aegypti (Ae.
30154681	9	52	from	period	1230:1235	arg1	activity					1259:1266	this activity	1254:1266	this activity	1254:1266	aegypti with high percentage for 27 d, whereas free O. gratissimum oil showed a short period of time (8 d) in this activity.
30154681	4	53	theme	beads	630:634	arg1	characteristics					611:625	Good characteristics	606:625	Good characteristics of beads	606:634	Good characteristics of beads were obtained by the orifice-ionic gelation method with calcium chloride as hardening agent and Tween®20 as emulsifier.
30154681	10	54	theme	oil	1313:1315	arg1	content					1317:1323	oil content	1313:1323	oil content	1313:1323	The stability study showed the stability of oil content and its compositions in storage condition.
30154681	6	55	theme	oil	877:879	arg1	content					881:887	The oil content	873:887	The oil content	873:887	The oil content, the yield percentage, and the entrapping efficiency were also examined.
30154681	3	56	theme	oil	440:442	arg1	analysis					413:420	Chemical analysis	404:420	Chemical analysis	404:420	Chemical analysis of O. gratissimum oil obtained by hydrodistillation, using gas chromatography-mass spectroscopy techniques, presented eugenol (67.38%) and Z-β-ocimene (14.95 %) as major constituents.
30154681	4	57	theme	calcium	692:698	arg1	chloride					700:707	calcium chloride	692:707	calcium chloride as hardening agent and Tween®20 as emulsifier	692:753	Good characteristics of beads were obtained by the orifice-ionic gelation method with calcium chloride as hardening agent and Tween®20 as emulsifier.
30154681	10	58	from	stability	1300:1308	arg1	condition					1357:1365	storage condition	1349:1365	storage condition	1349:1365	The stability study showed the stability of oil content and its compositions in storage condition.
30154681	0	59	theme	Product	74:80	arg1	Efficacy					22:29	Efficacy	22:29	Efficacy	22:29	Oviposition Deterrent Efficacy and Characteristics of a Botanical Natural Product, Ocimum gratissimum (L.) Oil-Alginate Beads, against Aedes aegypti (L.).
30154681	0	59	theme	Product	74:80	arg1	Characteristics					35:49	Characteristics	35:49	Characteristics	35:49	Oviposition Deterrent Efficacy and Characteristics of a Botanical Natural Product, Ocimum gratissimum (L.) Oil-Alginate Beads, against Aedes aegypti (L.).
30154681	5	60	theme	mm	869:870	arg1	size					849:852	an average size	838:852	an average size of 1.49 ± 1.36 mm	838:870	The beads exhibited a good spherical shape and good hardness and flexibility with an average size of 1.49 ± 1.36 mm.
30154681	4	61	theme	hardening	712:720	arg1	agent					722:726	hardening agent	712:726	hardening agent	712:726	Good characteristics of beads were obtained by the orifice-ionic gelation method with calcium chloride as hardening agent and Tween®20 as emulsifier.
30154681	1	62	theme	oviposition	197:207	arg1	activity					219:226	the oviposition deterrent activity	193:226	the oviposition deterrent activity of Ocimum gratissimum (L.) essential oil (O. gratissimum oil) and its product, Ocimum gratissimum (L.)- alginate beads (beads), against Aedes aegypti (Ae	193:380	This study was aimed at investigating the oviposition deterrent activity of Ocimum gratissimum (L.) essential oil (O. gratissimum oil) and its product, Ocimum gratissimum (L.)- alginate beads (beads), against Aedes aegypti (Ae.
30154681	0	63	theme	Natural	66:72	arg1	Product					74:80	a Botanical Natural Product	54:80	a Botanical Natural Product	54:80	Oviposition Deterrent Efficacy and Characteristics of a Botanical Natural Product, Ocimum gratissimum (L.) Oil-Alginate Beads, against Aedes aegypti (L.).
30154681	0	63	theme	Natural	66:72	arg1	Beads					120:124	Ocimum gratissimum (L.) Oil-Alginate Beads	83:124	Ocimum gratissimum (L.) Oil-Alginate Beads	83:124	Oviposition Deterrent Efficacy and Characteristics of a Botanical Natural Product, Ocimum gratissimum (L.) Oil-Alginate Beads, against Aedes aegypti (L.).
30154681	11	64	theme	dengue	1428:1433	arg1	fever					1435:1439	the dengue fever	1424:1439	the dengue fever	1424:1439	These results are very affordable approaches to control the dengue fever.
30154681	3	65	theme	O.	425:426	arg1	oil					440:442	O. gratissimum oil	425:442	O. gratissimum oil obtained by hydrodistillation	425:472	Chemical analysis of O. gratissimum oil obtained by hydrodistillation, using gas chromatography-mass spectroscopy techniques, presented eugenol (67.38%) and Z-β-ocimene (14.95 %) as major constituents.
30154681	1	66	theme	deterrent	209:217	arg1	activity					219:226	the oviposition deterrent activity	193:226	the oviposition deterrent activity of Ocimum gratissimum (L.) essential oil (O. gratissimum oil) and its product, Ocimum gratissimum (L.)- alginate beads (beads), against Aedes aegypti (Ae	193:380	This study was aimed at investigating the oviposition deterrent activity of Ocimum gratissimum (L.) essential oil (O. gratissimum oil) and its product, Ocimum gratissimum (L.)- alginate beads (beads), against Aedes aegypti (Ae.
30576805	9	0	theme	cell	1564:1567	arg1	wall					1569:1572	the cell wall	1560:1572	the cell wall in early development	1560:1593	Importantly, SUS4 may be responsible for arabinose and xylose biosynthesis to form the cell wall in early development, while SUS3 and VIN2 may be important in the accumulation of soluble hexose leading to cell expansion through an osmotic-independent pathway in late development.
30576805	7	1	theme	gene	1324:1327	arg1	expression					1329:1338	gene expression	1324:1338	gene expression	1324:1338	Also, the stability of 11 housekeeping genes were evaluated by geNorm method, resulting in a set of five genes (UBC, ACT, OR23, DWA2 and CYP21D) used as control for normalization of gene expression.
30576805	0	2	theme	genes	163:167	arg1	compositions					37:48	soluble sugar compositions	23:48	soluble sugar compositions	23:48	Fruit characteristics, soluble sugar compositions and transcriptome analysis during the development of Citrus maxima "seedless", and identification of SUS and INV genes involved in sucrose degradation.
30576805	0	2	theme	genes	163:167	arg1	analysis					68:75	transcriptome analysis	54:75	transcriptome analysis	54:75	Fruit characteristics, soluble sugar compositions and transcriptome analysis during the development of Citrus maxima "seedless", and identification of SUS and INV genes involved in sucrose degradation.
30576805	0	2	theme	genes	163:167	arg1	characteristics					6:20	Fruit characteristics	0:20	Fruit characteristics	0:20	Fruit characteristics, soluble sugar compositions and transcriptome analysis during the development of Citrus maxima "seedless", and identification of SUS and INV genes involved in sucrose degradation.
30576805	0	2	theme	genes	163:167	arg1	identification					133:146	identification	133:146	identification of SUS and INV genes	133:167	Fruit characteristics, soluble sugar compositions and transcriptome analysis during the development of Citrus maxima "seedless", and identification of SUS and INV genes involved in sucrose degradation.
30576805	2	3	theme	essential	316:324	arg1	features					326:333	The essential features	312:333	The essential features of this cultivar	312:350	The essential features of this cultivar are thin skin, green epicarp and seedless at the ripening stage.
30576805	2	3	theme	essential	316:324	arg1	skin					361:364	thin skin	356:364	thin skin	356:364	The essential features of this cultivar are thin skin, green epicarp and seedless at the ripening stage.
30576805	10	4	theme	fruit	1885:1889	arg1	yield					1891:1895	high fruit yield	1880:1895	high fruit yield	1880:1895	The information provides valuable metabolite and genetic resources in C. maxima "seedless", and is important for achieving high fruit yield and quality.
30576805	10	5	from	resources	1814:1822	arg1	"					1846:1846	C. maxima "seedless"	1827:1846	C. maxima "seedless"	1827:1846	The information provides valuable metabolite and genetic resources in C. maxima "seedless", and is important for achieving high fruit yield and quality.
30576805	0	6	theme	INV	159:161	arg1	genes					163:167	INV genes	159:167	INV genes	159:167	Fruit characteristics, soluble sugar compositions and transcriptome analysis during the development of Citrus maxima "seedless", and identification of SUS and INV genes involved in sucrose degradation.
30576805	9	7	from	pathway	1728:1734	arg1	development					1744:1754	late development	1739:1754	late development	1739:1754	Importantly, SUS4 may be responsible for arabinose and xylose biosynthesis to form the cell wall in early development, while SUS3 and VIN2 may be important in the accumulation of soluble hexose leading to cell expansion through an osmotic-independent pathway in late development.
30576805	3	8	theme	seedless	440:447	arg1	"					448:448	C. maxima "seedless"	429:448	C. maxima "seedless"	429:448	Here, using C. maxima "seedless" as experimental material, we investigated the physical and inclusion indicators, and found the accumulation of storage compounds during 120-210 DAF leading to inconsistent increase between volume and weight.
30576805	10	9	theme	high	1880:1883	arg1	yield					1891:1895	high fruit yield	1880:1895	high fruit yield	1880:1895	The information provides valuable metabolite and genetic resources in C. maxima "seedless", and is important for achieving high fruit yield and quality.
30576805	0	10	theme	Citrus	103:108	arg1	"					126:126	Citrus maxima "seedless"	103:126	Citrus maxima "seedless"	103:126	Fruit characteristics, soluble sugar compositions and transcriptome analysis during the development of Citrus maxima "seedless", and identification of SUS and INV genes involved in sucrose degradation.
30576805	5	11	theme	profiles	941:948	arg1	overview					909:916	a global overview	900:916	a global overview of the gene expressing profiles	900:948	To clarify a global overview of the gene expressing profiles, the PJSs from four periods (60, 120, 180 and 240 DAF) were selected for comparative transcriptome analysis.
30576805	6	12	theme	differential	1094:1105	arg1	expression					1107:1116	differential expression	1094:1116	differential expression	1094:1116	The resulting 8275 unigenes showed differential expression during PJS development.
30576805	9	13	theme	cell	1682:1685	arg1	expansion					1687:1695	cell expansion	1682:1695	cell expansion	1682:1695	Importantly, SUS4 may be responsible for arabinose and xylose biosynthesis to form the cell wall in early development, while SUS3 and VIN2 may be important in the accumulation of soluble hexose leading to cell expansion through an osmotic-independent pathway in late development.
30576805	10	14	theme	seedless	1838:1845	arg1	"					1846:1846	C. maxima "seedless"	1827:1846	C. maxima "seedless"	1827:1846	The information provides valuable metabolite and genetic resources in C. maxima "seedless", and is important for achieving high fruit yield and quality.
30576805	4	15	theme	soluble	680:686	arg1	sugar					688:692	soluble sugar	680:692	soluble sugar	680:692	Component analysis of soluble sugar indicated that arabinose and xylose have a high content in early development of pummelo juice sacs (PJS), whereas fructose, glucose and sucrose show a significant increase during PJS maturation.
30576805	2	16	from	stage	410:414	arg1	features					326:333	The essential features	312:333	The essential features of this cultivar	312:350	The essential features of this cultivar are thin skin, green epicarp and seedless at the ripening stage.
30576805	2	16	from	stage	410:414	arg1	seedless					385:392	seedless	385:392	seedless	385:392	The essential features of this cultivar are thin skin, green epicarp and seedless at the ripening stage.
30576805	2	16	from	stage	410:414	arg1	skin					361:364	thin skin	356:364	thin skin	356:364	The essential features of this cultivar are thin skin, green epicarp and seedless at the ripening stage.
30576805	2	16	from	stage	410:414	arg1	epicarp					373:379	green epicarp	367:379	green epicarp	367:379	The essential features of this cultivar are thin skin, green epicarp and seedless at the ripening stage.
30576805	10	17	theme	valuable	1782:1789	arg1	metabolite					1791:1800	valuable metabolite	1782:1800	valuable metabolite	1782:1800	The information provides valuable metabolite and genetic resources in C. maxima "seedless", and is important for achieving high fruit yield and quality.
30576805	5	18	from	periods	970:976	arg1	PJSs					955:958	the PJSs	951:958	the PJSs from four periods (60, 120, 180 and 240 DAF)	951:1003	To clarify a global overview of the gene expressing profiles, the PJSs from four periods (60, 120, 180 and 240 DAF) were selected for comparative transcriptome analysis.
30576805	8	19	theme	transcriptome	1350:1362	arg1	data					1364:1367	transcriptome data	1350:1367	transcriptome data	1350:1367	Based on transcriptome data, 5 sucrose synthases (SUSs) and 10 invertases (INVs) were identified to be involved in sucrose degradation.
30576805	4	20	theme	juice	782:786	arg1	sacs					788:791	pummelo juice sacs	774:791	pummelo juice sacs (PJS)	774:797	Component analysis of soluble sugar indicated that arabinose and xylose have a high content in early development of pummelo juice sacs (PJS), whereas fructose, glucose and sucrose show a significant increase during PJS maturation.
30576805	4	20	theme	juice	782:786	arg1	PJS					794:796	PJS	794:796	PJS	794:796	Component analysis of soluble sugar indicated that arabinose and xylose have a high content in early development of pummelo juice sacs (PJS), whereas fructose, glucose and sucrose show a significant increase during PJS maturation.
30576805	3	21	theme	experimental	453:464	arg1	material					466:473	experimental material	453:473	experimental material	453:473	Here, using C. maxima "seedless" as experimental material, we investigated the physical and inclusion indicators, and found the accumulation of storage compounds during 120-210 DAF leading to inconsistent increase between volume and weight.
30576805	7	22	theme	geNorm	1205:1210	arg1	method					1212:1217	geNorm method	1205:1217	geNorm method	1205:1217	Also, the stability of 11 housekeeping genes were evaluated by geNorm method, resulting in a set of five genes (UBC, ACT, OR23, DWA2 and CYP21D) used as control for normalization of gene expression.
30576805	2	23	theme	thin	356:359	arg1	features					326:333	The essential features	312:333	The essential features of this cultivar	312:350	The essential features of this cultivar are thin skin, green epicarp and seedless at the ripening stage.
30576805	2	23	theme	thin	356:359	arg1	skin					361:364	thin skin	356:364	thin skin	356:364	The essential features of this cultivar are thin skin, green epicarp and seedless at the ripening stage.
30576805	0	24	theme	maxima	110:115	arg1	"					126:126	Citrus maxima "seedless"	103:126	Citrus maxima "seedless"	103:126	Fruit characteristics, soluble sugar compositions and transcriptome analysis during the development of Citrus maxima "seedless", and identification of SUS and INV genes involved in sucrose degradation.
30576805	0	25	theme	sucrose	181:187	arg1	degradation					189:199	sucrose degradation	181:199	sucrose degradation	181:199	Fruit characteristics, soluble sugar compositions and transcriptome analysis during the development of Citrus maxima "seedless", and identification of SUS and INV genes involved in sucrose degradation.
30576805	10	26	from	metabolite	1791:1800	arg1	"					1846:1846	C. maxima "seedless"	1827:1846	C. maxima "seedless"	1827:1846	The information provides valuable metabolite and genetic resources in C. maxima "seedless", and is important for achieving high fruit yield and quality.
30576805	7	27	dep	genes	1247:1251	arg1	OR23					1264:1267	OR23	1264:1267	OR23	1264:1267	Also, the stability of 11 housekeeping genes were evaluated by geNorm method, resulting in a set of five genes (UBC, ACT, OR23, DWA2 and CYP21D) used as control for normalization of gene expression.
30576805	7	27	dep	genes	1247:1251	arg1	genes					1247:1251	five genes	1242:1251	five genes (UBC, ACT, OR23, DWA2 and CYP21D) used as control for normalization of gene expression	1242:1338	Also, the stability of 11 housekeeping genes were evaluated by geNorm method, resulting in a set of five genes (UBC, ACT, OR23, DWA2 and CYP21D) used as control for normalization of gene expression.
30576805	7	27	dep	genes	1247:1251	arg1	UBC					1254:1256	UBC	1254:1256	UBC	1254:1256	Also, the stability of 11 housekeeping genes were evaluated by geNorm method, resulting in a set of five genes (UBC, ACT, OR23, DWA2 and CYP21D) used as control for normalization of gene expression.
30576805	7	27	dep	genes	1247:1251	arg1	DWA2					1270:1273	DWA2	1270:1273	DWA2	1270:1273	Also, the stability of 11 housekeeping genes were evaluated by geNorm method, resulting in a set of five genes (UBC, ACT, OR23, DWA2 and CYP21D) used as control for normalization of gene expression.
30576805	7	27	dep	genes	1247:1251	arg1	CYP21D					1279:1284	CYP21D	1279:1284	CYP21D	1279:1284	Also, the stability of 11 housekeeping genes were evaluated by geNorm method, resulting in a set of five genes (UBC, ACT, OR23, DWA2 and CYP21D) used as control for normalization of gene expression.
30576805	7	27	dep	genes	1247:1251	arg1	ACT					1259:1261	ACT	1259:1261	ACT	1259:1261	Also, the stability of 11 housekeeping genes were evaluated by geNorm method, resulting in a set of five genes (UBC, ACT, OR23, DWA2 and CYP21D) used as control for normalization of gene expression.
30576805	3	28	theme	physical	496:503	arg1	indicators					519:528	the physical and inclusion indicators	492:528	indicators	519:528	Here, using C. maxima "seedless" as experimental material, we investigated the physical and inclusion indicators, and found the accumulation of storage compounds during 120-210 DAF leading to inconsistent increase between volume and weight.
30576805	9	29	theme	osmotic-independent	1708:1726	arg1	pathway					1728:1734	an osmotic-independent pathway	1705:1734	an osmotic-independent pathway in late development	1705:1754	Importantly, SUS4 may be responsible for arabinose and xylose biosynthesis to form the cell wall in early development, while SUS3 and VIN2 may be important in the accumulation of soluble hexose leading to cell expansion through an osmotic-independent pathway in late development.
30576805	2	30	theme	cultivar	343:350	arg1	features					326:333	The essential features	312:333	The essential features of this cultivar	312:350	The essential features of this cultivar are thin skin, green epicarp and seedless at the ripening stage.
30576805	2	30	theme	cultivar	343:350	arg1	skin					361:364	thin skin	356:364	thin skin	356:364	The essential features of this cultivar are thin skin, green epicarp and seedless at the ripening stage.
30576805	0	31	theme	Fruit	0:4	arg1	characteristics					6:20	Fruit characteristics	0:20	Fruit characteristics	0:20	Fruit characteristics, soluble sugar compositions and transcriptome analysis during the development of Citrus maxima "seedless", and identification of SUS and INV genes involved in sucrose degradation.
30576805	4	32	theme	sacs	788:791	arg1	development					759:769	early development	753:769	early development of pummelo juice sacs (PJS)	753:797	Component analysis of soluble sugar indicated that arabinose and xylose have a high content in early development of pummelo juice sacs (PJS), whereas fructose, glucose and sucrose show a significant increase during PJS maturation.
30576805	6	33	theme	PJS	1125:1127	arg1	development					1129:1139	PJS development	1125:1139	PJS development	1125:1139	The resulting 8275 unigenes showed differential expression during PJS development.
30576805	10	34	theme	maxima	1830:1835	arg1	"					1846:1846	C. maxima "seedless"	1827:1846	C. maxima "seedless"	1827:1846	The information provides valuable metabolite and genetic resources in C. maxima "seedless", and is important for achieving high fruit yield and quality.
30576805	4	35	dep	have	730:733	arg1	whereas					800:806	whereas	800:806	whereas	800:806	Component analysis of soluble sugar indicated that arabinose and xylose have a high content in early development of pummelo juice sacs (PJS), whereas fructose, glucose and sucrose show a significant increase during PJS maturation.
30576805	0	36	theme	soluble	23:29	arg1	compositions					37:48	soluble sugar compositions	23:48	soluble sugar compositions	23:48	Fruit characteristics, soluble sugar compositions and transcriptome analysis during the development of Citrus maxima "seedless", and identification of SUS and INV genes involved in sucrose degradation.
30576805	8	37	theme	sucrose	1372:1378	arg1	SUSs					1391:1394	SUSs	1391:1394	SUSs	1391:1394	Based on transcriptome data, 5 sucrose synthases (SUSs) and 10 invertases (INVs) were identified to be involved in sucrose degradation.
30576805	8	37	theme	sucrose	1372:1378	arg1	synthases					1380:1388	5 sucrose synthases	1370:1388	5 sucrose synthases (SUSs)	1370:1395	Based on transcriptome data, 5 sucrose synthases (SUSs) and 10 invertases (INVs) were identified to be involved in sucrose degradation.
30576805	0	38	theme	"	126:126	arg1	development					88:98	the development	84:98	the development of Citrus maxima "seedless"	84:126	Fruit characteristics, soluble sugar compositions and transcriptome analysis during the development of Citrus maxima "seedless", and identification of SUS and INV genes involved in sucrose degradation.
30576805	7	39	theme	genes	1247:1251	arg1	OR23					1264:1267	OR23	1264:1267	OR23	1264:1267	Also, the stability of 11 housekeeping genes were evaluated by geNorm method, resulting in a set of five genes (UBC, ACT, OR23, DWA2 and CYP21D) used as control for normalization of gene expression.
30576805	7	39	theme	genes	1247:1251	arg1	genes					1247:1251	five genes	1242:1251	five genes (UBC, ACT, OR23, DWA2 and CYP21D) used as control for normalization of gene expression	1242:1338	Also, the stability of 11 housekeeping genes were evaluated by geNorm method, resulting in a set of five genes (UBC, ACT, OR23, DWA2 and CYP21D) used as control for normalization of gene expression.
30576805	7	39	theme	genes	1247:1251	arg1	UBC					1254:1256	UBC	1254:1256	UBC	1254:1256	Also, the stability of 11 housekeeping genes were evaluated by geNorm method, resulting in a set of five genes (UBC, ACT, OR23, DWA2 and CYP21D) used as control for normalization of gene expression.
30576805	7	39	theme	genes	1247:1251	arg1	DWA2					1270:1273	DWA2	1270:1273	DWA2	1270:1273	Also, the stability of 11 housekeeping genes were evaluated by geNorm method, resulting in a set of five genes (UBC, ACT, OR23, DWA2 and CYP21D) used as control for normalization of gene expression.
30576805	7	39	theme	genes	1247:1251	arg1	CYP21D					1279:1284	CYP21D	1279:1284	CYP21D	1279:1284	Also, the stability of 11 housekeeping genes were evaluated by geNorm method, resulting in a set of five genes (UBC, ACT, OR23, DWA2 and CYP21D) used as control for normalization of gene expression.
30576805	7	39	theme	genes	1247:1251	arg1	set					1235:1237	a set	1233:1237	a set of five genes (UBC, ACT, OR23, DWA2 and CYP21D) used as control for normalization of gene expression	1233:1338	Also, the stability of 11 housekeeping genes were evaluated by geNorm method, resulting in a set of five genes (UBC, ACT, OR23, DWA2 and CYP21D) used as control for normalization of gene expression.
30576805	5	40	theme	gene	925:928	arg1	profiles					941:948	the gene expressing profiles	921:948	the gene expressing profiles	921:948	To clarify a global overview of the gene expressing profiles, the PJSs from four periods (60, 120, 180 and 240 DAF) were selected for comparative transcriptome analysis.
30576805	0	41	theme	seedless	118:125	arg1	"					126:126	Citrus maxima "seedless"	103:126	Citrus maxima "seedless"	103:126	Fruit characteristics, soluble sugar compositions and transcriptome analysis during the development of Citrus maxima "seedless", and identification of SUS and INV genes involved in sucrose degradation.
30576805	4	42	theme	sugar	688:692	arg1	analysis					668:675	Component analysis	658:675	Component analysis of soluble sugar	658:692	Component analysis of soluble sugar indicated that arabinose and xylose have a high content in early development of pummelo juice sacs (PJS), whereas fructose, glucose and sucrose show a significant increase during PJS maturation.
30576805	9	43	theme	late	1739:1742	arg1	development					1744:1754	late development	1739:1754	late development	1739:1754	Importantly, SUS4 may be responsible for arabinose and xylose biosynthesis to form the cell wall in early development, while SUS3 and VIN2 may be important in the accumulation of soluble hexose leading to cell expansion through an osmotic-independent pathway in late development.
30576805	2	44	theme	green	367:371	arg1	epicarp					373:379	green epicarp	367:379	green epicarp	367:379	The essential features of this cultivar are thin skin, green epicarp and seedless at the ripening stage.
30576805	7	45	theme	housekeeping	1168:1179	arg1	genes					1181:1185	11 housekeeping genes	1165:1185	11 housekeeping genes	1165:1185	Also, the stability of 11 housekeeping genes were evaluated by geNorm method, resulting in a set of five genes (UBC, ACT, OR23, DWA2 and CYP21D) used as control for normalization of gene expression.
30576805	4	46	theme	significant	845:855	arg1	increase					857:864	a significant increase	843:864	a significant increase during PJS maturation	843:886	Component analysis of soluble sugar indicated that arabinose and xylose have a high content in early development of pummelo juice sacs (PJS), whereas fructose, glucose and sucrose show a significant increase during PJS maturation.
30576805	4	47	theme	early	753:757	arg1	development					759:769	early development	753:769	early development of pummelo juice sacs (PJS)	753:797	Component analysis of soluble sugar indicated that arabinose and xylose have a high content in early development of pummelo juice sacs (PJS), whereas fructose, glucose and sucrose show a significant increase during PJS maturation.
30576805	7	48	theme	genes	1181:1185	arg1	stability					1152:1160	the stability	1148:1160	the stability of 11 housekeeping genes	1148:1185	Also, the stability of 11 housekeeping genes were evaluated by geNorm method, resulting in a set of five genes (UBC, ACT, OR23, DWA2 and CYP21D) used as control for normalization of gene expression.
30576805	0	49	theme	sugar	31:35	arg1	compositions					37:48	soluble sugar compositions	23:48	soluble sugar compositions	23:48	Fruit characteristics, soluble sugar compositions and transcriptome analysis during the development of Citrus maxima "seedless", and identification of SUS and INV genes involved in sucrose degradation.
30576805	10	50	theme	genetic	1806:1812	arg1	resources					1814:1822	genetic resources	1806:1822	genetic resources	1806:1822	The information provides valuable metabolite and genetic resources in C. maxima "seedless", and is important for achieving high fruit yield and quality.
30576805	3	51	theme	storage	561:567	arg1	compounds					569:577	storage compounds	561:577	storage compounds	561:577	Here, using C. maxima "seedless" as experimental material, we investigated the physical and inclusion indicators, and found the accumulation of storage compounds during 120-210 DAF leading to inconsistent increase between volume and weight.
30576805	4	52	contain	have	730:733	arg1	arabinose					709:717	arabinose	709:717	arabinose	709:717	Component analysis of soluble sugar indicated that arabinose and xylose have a high content in early development of pummelo juice sacs (PJS), whereas fructose, glucose and sucrose show a significant increase during PJS maturation.
30576805	4	52	contain	have	730:733	arg2	content					742:748	a high content	735:748	a high content in early development of pummelo juice sacs (PJS)	735:797	Component analysis of soluble sugar indicated that arabinose and xylose have a high content in early development of pummelo juice sacs (PJS), whereas fructose, glucose and sucrose show a significant increase during PJS maturation.
30576805	4	52	contain	have	730:733	arg1	xylose					723:728	xylose	723:728	xylose	723:728	Component analysis of soluble sugar indicated that arabinose and xylose have a high content in early development of pummelo juice sacs (PJS), whereas fructose, glucose and sucrose show a significant increase during PJS maturation.
30576805	1	53	theme	Citrus	202:207	arg1	"					225:225	Citrus maxima "seedless"	202:225	Citrus maxima "seedless"	202:225	Citrus maxima "seedless" is originally from Malaysia, and now is widely cultivated in Hainan province, China.
30576805	0	54	theme	transcriptome	54:66	arg1	analysis					68:75	transcriptome analysis	54:75	transcriptome analysis	54:75	Fruit characteristics, soluble sugar compositions and transcriptome analysis during the development of Citrus maxima "seedless", and identification of SUS and INV genes involved in sucrose degradation.
30576805	3	55	theme	compounds	569:577	arg1	accumulation					545:556	the accumulation	541:556	the accumulation of storage compounds	541:577	Here, using C. maxima "seedless" as experimental material, we investigated the physical and inclusion indicators, and found the accumulation of storage compounds during 120-210 DAF leading to inconsistent increase between volume and weight.
30576805	3	56	theme	inclusion	509:517	arg1	indicators					519:528	the physical and inclusion indicators	492:528	indicators	519:528	Here, using C. maxima "seedless" as experimental material, we investigated the physical and inclusion indicators, and found the accumulation of storage compounds during 120-210 DAF leading to inconsistent increase between volume and weight.
30576805	3	57	theme	C.	429:430	arg1	"					448:448	C. maxima "seedless"	429:448	C. maxima "seedless"	429:448	Here, using C. maxima "seedless" as experimental material, we investigated the physical and inclusion indicators, and found the accumulation of storage compounds during 120-210 DAF leading to inconsistent increase between volume and weight.
30576805	2	58	theme	ripening	401:408	arg1	stage					410:414	the ripening stage	397:414	the ripening stage	397:414	The essential features of this cultivar are thin skin, green epicarp and seedless at the ripening stage.
30576805	5	59	theme	comparative	1023:1033	arg1	analysis					1049:1056	comparative transcriptome analysis	1023:1056	comparative transcriptome analysis	1023:1056	To clarify a global overview of the gene expressing profiles, the PJSs from four periods (60, 120, 180 and 240 DAF) were selected for comparative transcriptome analysis.
30576805	9	60	theme	xylose	1532:1537	arg1	biosynthesis					1539:1550	xylose biosynthesis	1532:1550	xylose biosynthesis	1532:1550	Importantly, SUS4 may be responsible for arabinose and xylose biosynthesis to form the cell wall in early development, while SUS3 and VIN2 may be important in the accumulation of soluble hexose leading to cell expansion through an osmotic-independent pathway in late development.
30576805	9	61	from	wall	1569:1572	arg1	development					1583:1593	early development	1577:1593	early development	1577:1593	Importantly, SUS4 may be responsible for arabinose and xylose biosynthesis to form the cell wall in early development, while SUS3 and VIN2 may be important in the accumulation of soluble hexose leading to cell expansion through an osmotic-independent pathway in late development.
30576805	4	62	theme	pummelo	774:780	arg1	sacs					788:791	pummelo juice sacs	774:791	pummelo juice sacs (PJS)	774:797	Component analysis of soluble sugar indicated that arabinose and xylose have a high content in early development of pummelo juice sacs (PJS), whereas fructose, glucose and sucrose show a significant increase during PJS maturation.
30576805	4	62	theme	pummelo	774:780	arg1	PJS					794:796	PJS	794:796	PJS	794:796	Component analysis of soluble sugar indicated that arabinose and xylose have a high content in early development of pummelo juice sacs (PJS), whereas fructose, glucose and sucrose show a significant increase during PJS maturation.
30576805	9	63	from	accumulation	1640:1651	arg1	important					1623:1631	important	1623:1631	important	1623:1631	Importantly, SUS4 may be responsible for arabinose and xylose biosynthesis to form the cell wall in early development, while SUS3 and VIN2 may be important in the accumulation of soluble hexose leading to cell expansion through an osmotic-independent pathway in late development.
30576805	3	64	theme	maxima	432:437	arg1	"					448:448	C. maxima "seedless"	429:448	C. maxima "seedless"	429:448	Here, using C. maxima "seedless" as experimental material, we investigated the physical and inclusion indicators, and found the accumulation of storage compounds during 120-210 DAF leading to inconsistent increase between volume and weight.
30576805	8	65	theme	sucrose	1456:1462	arg1	degradation					1464:1474	sucrose degradation	1456:1474	sucrose degradation	1456:1474	Based on transcriptome data, 5 sucrose synthases (SUSs) and 10 invertases (INVs) were identified to be involved in sucrose degradation.
30576805	5	66	theme	transcriptome	1035:1047	arg1	analysis					1049:1056	comparative transcriptome analysis	1023:1056	comparative transcriptome analysis	1023:1056	To clarify a global overview of the gene expressing profiles, the PJSs from four periods (60, 120, 180 and 240 DAF) were selected for comparative transcriptome analysis.
30576805	3	67	theme	inconsistent	609:620	arg1	increase					622:629	inconsistent increase	609:629	inconsistent increase between volume and weight	609:655	Here, using C. maxima "seedless" as experimental material, we investigated the physical and inclusion indicators, and found the accumulation of storage compounds during 120-210 DAF leading to inconsistent increase between volume and weight.
30576805	5	68	theme	global	902:907	arg1	overview					909:916	a global overview	900:916	a global overview of the gene expressing profiles	900:948	To clarify a global overview of the gene expressing profiles, the PJSs from four periods (60, 120, 180 and 240 DAF) were selected for comparative transcriptome analysis.
30576805	5	69	theme	expressing	930:939	arg1	profiles					941:948	the gene expressing profiles	921:948	the gene expressing profiles	921:948	To clarify a global overview of the gene expressing profiles, the PJSs from four periods (60, 120, 180 and 240 DAF) were selected for comparative transcriptome analysis.
30576805	1	70	theme	maxima	209:214	arg1	"					225:225	Citrus maxima "seedless"	202:225	Citrus maxima "seedless"	202:225	Citrus maxima "seedless" is originally from Malaysia, and now is widely cultivated in Hainan province, China.
30576805	0	71	theme	SUS	151:153	arg1	compositions					37:48	soluble sugar compositions	23:48	soluble sugar compositions	23:48	Fruit characteristics, soluble sugar compositions and transcriptome analysis during the development of Citrus maxima "seedless", and identification of SUS and INV genes involved in sucrose degradation.
30576805	0	71	theme	SUS	151:153	arg1	analysis					68:75	transcriptome analysis	54:75	transcriptome analysis	54:75	Fruit characteristics, soluble sugar compositions and transcriptome analysis during the development of Citrus maxima "seedless", and identification of SUS and INV genes involved in sucrose degradation.
30576805	0	71	theme	SUS	151:153	arg1	characteristics					6:20	Fruit characteristics	0:20	Fruit characteristics	0:20	Fruit characteristics, soluble sugar compositions and transcriptome analysis during the development of Citrus maxima "seedless", and identification of SUS and INV genes involved in sucrose degradation.
30576805	0	71	theme	SUS	151:153	arg1	identification					133:146	identification	133:146	identification of SUS and INV genes	133:167	Fruit characteristics, soluble sugar compositions and transcriptome analysis during the development of Citrus maxima "seedless", and identification of SUS and INV genes involved in sucrose degradation.
30576805	7	72	theme	expression	1329:1338	arg1	normalization					1307:1319	normalization	1307:1319	normalization of gene expression	1307:1338	Also, the stability of 11 housekeeping genes were evaluated by geNorm method, resulting in a set of five genes (UBC, ACT, OR23, DWA2 and CYP21D) used as control for normalization of gene expression.
30576805	6	73	theme	resulting	1063:1071	arg1	unigenes					1078:1085	The resulting 8275 unigenes	1059:1085	The resulting 8275 unigenes	1059:1085	The resulting 8275 unigenes showed differential expression during PJS development.
30576805	4	74	from	content	742:748	arg1	development					759:769	early development	753:769	early development of pummelo juice sacs (PJS)	753:797	Component analysis of soluble sugar indicated that arabinose and xylose have a high content in early development of pummelo juice sacs (PJS), whereas fructose, glucose and sucrose show a significant increase during PJS maturation.
30576805	4	75	theme	Component	658:666	arg1	analysis					668:675	Component analysis	658:675	Component analysis of soluble sugar	658:692	Component analysis of soluble sugar indicated that arabinose and xylose have a high content in early development of pummelo juice sacs (PJS), whereas fructose, glucose and sucrose show a significant increase during PJS maturation.
30576805	10	76	theme	C.	1827:1828	arg1	"					1846:1846	C. maxima "seedless"	1827:1846	C. maxima "seedless"	1827:1846	The information provides valuable metabolite and genetic resources in C. maxima "seedless", and is important for achieving high fruit yield and quality.
30576805	9	77	theme	soluble	1656:1662	arg1	hexose					1664:1669	soluble hexose	1656:1669	soluble hexose	1656:1669	Importantly, SUS4 may be responsible for arabinose and xylose biosynthesis to form the cell wall in early development, while SUS3 and VIN2 may be important in the accumulation of soluble hexose leading to cell expansion through an osmotic-independent pathway in late development.
30576805	1	78	theme	seedless	217:224	arg1	"					225:225	Citrus maxima "seedless"	202:225	Citrus maxima "seedless"	202:225	Citrus maxima "seedless" is originally from Malaysia, and now is widely cultivated in Hainan province, China.
30576805	9	79	from	important	1623:1631	arg1	accumulation					1640:1651	the accumulation	1636:1651	the accumulation of soluble hexose leading to cell expansion through an osmotic-independent pathway in late development	1636:1754	Importantly, SUS4 may be responsible for arabinose and xylose biosynthesis to form the cell wall in early development, while SUS3 and VIN2 may be important in the accumulation of soluble hexose leading to cell expansion through an osmotic-independent pathway in late development.
30576805	4	80	theme	high	737:740	arg1	content					742:748	a high content	735:748	a high content in early development of pummelo juice sacs (PJS)	735:797	Component analysis of soluble sugar indicated that arabinose and xylose have a high content in early development of pummelo juice sacs (PJS), whereas fructose, glucose and sucrose show a significant increase during PJS maturation.
30576805	4	81	theme	PJS	873:875	arg1	maturation					877:886	PJS maturation	873:886	PJS maturation	873:886	Component analysis of soluble sugar indicated that arabinose and xylose have a high content in early development of pummelo juice sacs (PJS), whereas fructose, glucose and sucrose show a significant increase during PJS maturation.
30576805	9	82	theme	hexose	1664:1669	arg1	accumulation					1640:1651	the accumulation	1636:1651	the accumulation of soluble hexose leading to cell expansion through an osmotic-independent pathway in late development	1636:1754	Importantly, SUS4 may be responsible for arabinose and xylose biosynthesis to form the cell wall in early development, while SUS3 and VIN2 may be important in the accumulation of soluble hexose leading to cell expansion through an osmotic-independent pathway in late development.
30576805	9	83	theme	early	1577:1581	arg1	development					1583:1593	early development	1577:1593	early development	1577:1593	Importantly, SUS4 may be responsible for arabinose and xylose biosynthesis to form the cell wall in early development, while SUS3 and VIN2 may be important in the accumulation of soluble hexose leading to cell expansion through an osmotic-independent pathway in late development.
30068880	0	0	theme	Encapsulated	102:113	arg1	siRNA					115:119	Encapsulated siRNA	102:119	Encapsulated siRNA	102:119	Development of an Alcohol Dilution-Lyophilization Method for Preparing Lipid Nanoparticles Containing Encapsulated siRNA.
30068880	8	1	theme	sucrose	1078:1084	arg1	combination					1063:1073	A combination	1061:1073	A combination of sucrose and 1-(monomethoxy polyethyleneglycol5000)-2,3-dimyristoylglycerol (DMG-PEG5000)	1061:1165	A combination of sucrose and 1-(monomethoxy polyethyleneglycol5000)-2,3-dimyristoylglycerol (DMG-PEG5000) was found to have the most efficient cryoprotective activity for the LNPs.
30068880	7	2	theme	view	1029:1032	arg1	point					1020:1024	the point	1016:1024	the point of view of particle stabilization	1016:1058	At first, the compositions of cryoprotectants and polyethylene glycol (PEG)-lipids that were used were optimized from the point of view of particle stabilization.
30068880	9	3	theme	alcohol	1294:1300	arg1	method					1326:1331	the alcohol dilution-lyophilization method	1290:1331	the alcohol dilution-lyophilization method	1290:1331	The knockdown efficiency of the LNP prepared by the alcohol dilution-lyophilization method was comparable to that of an LNP prepared by the conventional ultrafiltration method.
30068880	9	4	theme	LNP	1274:1276	arg1	efficiency					1256:1265	The knockdown efficiency	1242:1265	The knockdown efficiency of the LNP prepared by the alcohol dilution-lyophilization method	1242:1331	The knockdown efficiency of the LNP prepared by the alcohol dilution-lyophilization method was comparable to that of an LNP prepared by the conventional ultrafiltration method.
30068880	9	4	theme	LNP	1274:1276	arg1	comparable					1337:1346	comparable	1337:1346	comparable	1337:1346	The knockdown efficiency of the LNP prepared by the alcohol dilution-lyophilization method was comparable to that of an LNP prepared by the conventional ultrafiltration method.
30068880	7	5	theme	particle	1037:1044	arg1	stabilization					1046:1058	particle stabilization	1037:1058	particle stabilization	1037:1058	At first, the compositions of cryoprotectants and polyethylene glycol (PEG)-lipids that were used were optimized from the point of view of particle stabilization.
30068880	9	6	theme	dilution-lyophilization	1302:1324	arg1	method					1326:1331	the alcohol dilution-lyophilization method	1290:1331	the alcohol dilution-lyophilization method	1290:1331	The knockdown efficiency of the LNP prepared by the alcohol dilution-lyophilization method was comparable to that of an LNP prepared by the conventional ultrafiltration method.
30068880	7	7	theme	-lipids	973:979	arg1	compositions					912:923	the compositions	908:923	the compositions of cryoprotectants and polyethylene glycol (PEG)-lipids that were used	908:994	At first, the compositions of cryoprotectants and polyethylene glycol (PEG)-lipids that were used were optimized from the point of view of particle stabilization.
30068880	6	8	dep	use	769:771	arg1	concentrate					869:879	concentrate	869:879	to simultaneously concentrate the preparation	851:895	This method involves the use of a freeze-drying (lyophilization) method to remove the residual alcohol and to simultaneously concentrate the preparation.
30068880	6	8	dep	use	769:771	arg1	remove					819:824	remove	819:824	to remove the residual alcohol	816:845	This method involves the use of a freeze-drying (lyophilization) method to remove the residual alcohol and to simultaneously concentrate the preparation.
30068880	4	9	from	necessary	520:528	arg1	method					506:511	this method	501:511	this method	501:511	In this method, it is necessary to remove the alcohol and then concentrate the LNP sample before they can be used.
30068880	6	10	theme	method	809:814	arg1	use					769:771	the use	765:771	the use of a freeze-drying (lyophilization) method to remove the residual alcohol and to simultaneously concentrate the preparation	765:895	This method involves the use of a freeze-drying (lyophilization) method to remove the residual alcohol and to simultaneously concentrate the preparation.
30068880	8	11	contain	have	1180:1183	arg2	activity					1219:1226	the most efficient cryoprotective activity	1185:1226	the most efficient cryoprotective activity for the LNPs	1185:1239	A combination of sucrose and 1-(monomethoxy polyethyleneglycol5000)-2,3-dimyristoylglycerol (DMG-PEG5000) was found to have the most efficient cryoprotective activity for the LNPs.
30068880	8	11	contain	have	1180:1183	arg1	combination					1063:1073	A combination	1061:1073	A combination of sucrose and 1-(monomethoxy polyethyleneglycol5000)-2,3-dimyristoylglycerol (DMG-PEG5000)	1061:1165	A combination of sucrose and 1-(monomethoxy polyethyleneglycol5000)-2,3-dimyristoylglycerol (DMG-PEG5000) was found to have the most efficient cryoprotective activity for the LNPs.
30068880	3	12	theme	alcohol	473:479	arg1	method					490:495	an alcohol dilution method	470:495	an alcohol dilution method	470:495	LNPs are generally prepared by an alcohol dilution method.
30068880	6	13	theme	lyophilization	793:806	arg1	method					809:814	a freeze-drying (lyophilization) method	776:814	a freeze-drying (lyophilization) method	776:814	This method involves the use of a freeze-drying (lyophilization) method to remove the residual alcohol and to simultaneously concentrate the preparation.
30068880	1	14	theme	personalized	206:217	arg1	medicine					219:226	personalized medicine	206:226	personalized medicine	206:226	Systems for delivering nucleic acids are now fundamental technologies for realizing personalized medicine.
30068880	7	15	theme	polyethylene	948:959	arg1	PEG					969:971	PEG	969:971	PEG	969:971	At first, the compositions of cryoprotectants and polyethylene glycol (PEG)-lipids that were used were optimized from the point of view of particle stabilization.
30068880	7	15	theme	polyethylene	948:959	arg1	glycol					961:966	polyethylene glycol	948:966	polyethylene glycol (PEG)-lipids	948:979	At first, the compositions of cryoprotectants and polyethylene glycol (PEG)-lipids that were used were optimized from the point of view of particle stabilization.
30068880	7	16	theme	glycol	961:966	arg1	-lipids					973:979	polyethylene glycol (PEG)-lipids	948:979	polyethylene glycol (PEG)-lipids	948:979	At first, the compositions of cryoprotectants and polyethylene glycol (PEG)-lipids that were used were optimized from the point of view of particle stabilization.
30068880	2	17	theme	interfering	353:363	arg1	siRNA					370:374	siRNA	370:374	siRNA	370:374	Among the various nucleic acid delivery systems that are currently available, lipid-nanoparticles (LNPs) that contain short interfering RNA (siRNA) have been extensively investigated for clinical applications.
30068880	2	17	theme	interfering	353:363	arg1	RNA					365:367	short interfering RNA	347:367	short interfering RNA (siRNA)	347:375	Among the various nucleic acid delivery systems that are currently available, lipid-nanoparticles (LNPs) that contain short interfering RNA (siRNA) have been extensively investigated for clinical applications.
30068880	5	18	theme	alcohol	664:670	arg1	"					702:702	an "alcohol dilution-lyophilization method"	660:702	an "alcohol dilution-lyophilization method" for preparing siRNA-encapsulating LNPs	660:741	In this study, we report on the development of an "alcohol dilution-lyophilization method" for preparing siRNA-encapsulating LNPs.
30068880	2	19	theme	clinical	416:423	arg1	applications					425:436	clinical applications	416:436	clinical applications	416:436	Among the various nucleic acid delivery systems that are currently available, lipid-nanoparticles (LNPs) that contain short interfering RNA (siRNA) have been extensively investigated for clinical applications.
30068880	4	20	theme	LNP	577:579	arg1	sample					581:586	the LNP sample	573:586	the LNP sample	573:586	In this method, it is necessary to remove the alcohol and then concentrate the LNP sample before they can be used.
30068880	4	21	from	method	506:511	arg1	necessary					520:528	necessary	520:528	necessary	520:528	In this method, it is necessary to remove the alcohol and then concentrate the LNP sample before they can be used.
30068880	6	22	theme	freeze-drying	778:790	arg1	method					809:814	a freeze-drying (lyophilization) method	776:814	a freeze-drying (lyophilization) method	776:814	This method involves the use of a freeze-drying (lyophilization) method to remove the residual alcohol and to simultaneously concentrate the preparation.
30068880	2	23	theme	short	347:351	arg1	siRNA					370:374	siRNA	370:374	siRNA	370:374	Among the various nucleic acid delivery systems that are currently available, lipid-nanoparticles (LNPs) that contain short interfering RNA (siRNA) have been extensively investigated for clinical applications.
30068880	2	23	theme	short	347:351	arg1	RNA					365:367	short interfering RNA	347:367	short interfering RNA (siRNA)	347:375	Among the various nucleic acid delivery systems that are currently available, lipid-nanoparticles (LNPs) that contain short interfering RNA (siRNA) have been extensively investigated for clinical applications.
30068880	0	24	theme	Alcohol	18:24	arg1	Method					50:55	an Alcohol Dilution-Lyophilization Method	15:55	an Alcohol Dilution-Lyophilization Method	15:55	Development of an Alcohol Dilution-Lyophilization Method for Preparing Lipid Nanoparticles Containing Encapsulated siRNA.
30068880	7	25	theme	cryoprotectants	928:942	arg1	compositions					912:923	the compositions	908:923	the compositions of cryoprotectants and polyethylene glycol (PEG)-lipids that were used	908:994	At first, the compositions of cryoprotectants and polyethylene glycol (PEG)-lipids that were used were optimized from the point of view of particle stabilization.
30068880	5	26	theme	"	702:702	arg1	development					645:655	the development	641:655	the development of an "alcohol dilution-lyophilization method" for preparing siRNA-encapsulating LNPs	641:741	In this study, we report on the development of an "alcohol dilution-lyophilization method" for preparing siRNA-encapsulating LNPs.
30068880	8	27	theme	efficient	1194:1202	arg1	activity					1219:1226	the most efficient cryoprotective activity	1185:1226	the most efficient cryoprotective activity for the LNPs	1185:1239	A combination of sucrose and 1-(monomethoxy polyethyleneglycol5000)-2,3-dimyristoylglycerol (DMG-PEG5000) was found to have the most efficient cryoprotective activity for the LNPs.
30068880	0	28	theme	Method	50:55	arg1	Development					0:10	Development	0:10	Development of an Alcohol Dilution-Lyophilization Method for Preparing Lipid Nanoparticles	0:89	Development of an Alcohol Dilution-Lyophilization Method for Preparing Lipid Nanoparticles Containing Encapsulated siRNA.
30068880	7	29	theme	stabilization	1046:1058	arg1	view					1029:1032	view	1029:1032	view of particle stabilization	1029:1058	At first, the compositions of cryoprotectants and polyethylene glycol (PEG)-lipids that were used were optimized from the point of view of particle stabilization.
30068880	0	30	theme	Dilution-Lyophilization	26:48	arg1	Method					50:55	an Alcohol Dilution-Lyophilization Method	15:55	an Alcohol Dilution-Lyophilization Method	15:55	Development of an Alcohol Dilution-Lyophilization Method for Preparing Lipid Nanoparticles Containing Encapsulated siRNA.
30068880	5	31	theme	siRNA-encapsulating	718:736	arg1	LNPs					738:741	siRNA-encapsulating LNPs	718:741	siRNA-encapsulating LNPs	718:741	In this study, we report on the development of an "alcohol dilution-lyophilization method" for preparing siRNA-encapsulating LNPs.
30068880	9	32	theme	knockdown	1246:1254	arg1	efficiency					1256:1265	The knockdown efficiency	1242:1265	The knockdown efficiency of the LNP prepared by the alcohol dilution-lyophilization method	1242:1331	The knockdown efficiency of the LNP prepared by the alcohol dilution-lyophilization method was comparable to that of an LNP prepared by the conventional ultrafiltration method.
30068880	9	32	theme	knockdown	1246:1254	arg1	comparable					1337:1346	comparable	1337:1346	comparable	1337:1346	The knockdown efficiency of the LNP prepared by the alcohol dilution-lyophilization method was comparable to that of an LNP prepared by the conventional ultrafiltration method.
30068880	8	33	theme	cryoprotective	1204:1217	arg1	activity					1219:1226	the most efficient cryoprotective activity	1185:1226	the most efficient cryoprotective activity for the LNPs	1185:1239	A combination of sucrose and 1-(monomethoxy polyethyleneglycol5000)-2,3-dimyristoylglycerol (DMG-PEG5000) was found to have the most efficient cryoprotective activity for the LNPs.
30068880	2	34	contain	contain	339:345	arg1	LNPs					328:331	LNPs	328:331	LNPs	328:331	Among the various nucleic acid delivery systems that are currently available, lipid-nanoparticles (LNPs) that contain short interfering RNA (siRNA) have been extensively investigated for clinical applications.
30068880	2	34	contain	contain	339:345	arg2	RNA					365:367	short interfering RNA	347:367	short interfering RNA (siRNA)	347:375	Among the various nucleic acid delivery systems that are currently available, lipid-nanoparticles (LNPs) that contain short interfering RNA (siRNA) have been extensively investigated for clinical applications.
30068880	2	34	contain	contain	339:345	arg1	lipid-nanoparticles					307:325	lipid-nanoparticles	307:325	lipid-nanoparticles (LNPs) that contain short interfering RNA (siRNA)	307:375	Among the various nucleic acid delivery systems that are currently available, lipid-nanoparticles (LNPs) that contain short interfering RNA (siRNA) have been extensively investigated for clinical applications.
30068880	2	34	contain	contain	339:345	arg2	siRNA					370:374	siRNA	370:374	siRNA	370:374	Among the various nucleic acid delivery systems that are currently available, lipid-nanoparticles (LNPs) that contain short interfering RNA (siRNA) have been extensively investigated for clinical applications.
30068880	8	35	theme	monomethoxy	1093:1103	arg1	-2,3-dimyristoylglycerol					1128:1151	1-(monomethoxy polyethyleneglycol5000)-2,3-dimyristoylglycerol (DMG-PEG5000)	1090:1165	1-(monomethoxy polyethyleneglycol5000)-2,3-dimyristoylglycerol (DMG-PEG5000)	1090:1165	A combination of sucrose and 1-(monomethoxy polyethyleneglycol5000)-2,3-dimyristoylglycerol (DMG-PEG5000) was found to have the most efficient cryoprotective activity for the LNPs.
30068880	8	36	theme	-2,3-dimyristoylglycerol	1128:1151	arg1	combination					1063:1073	A combination	1061:1073	A combination of sucrose and 1-(monomethoxy polyethyleneglycol5000)-2,3-dimyristoylglycerol (DMG-PEG5000)	1061:1165	A combination of sucrose and 1-(monomethoxy polyethyleneglycol5000)-2,3-dimyristoylglycerol (DMG-PEG5000) was found to have the most efficient cryoprotective activity for the LNPs.
30068880	2	37	theme	delivery	260:267	arg1	systems					269:275	the various nucleic acid delivery systems	235:275	the various nucleic acid delivery systems that are currently available	235:304	Among the various nucleic acid delivery systems that are currently available, lipid-nanoparticles (LNPs) that contain short interfering RNA (siRNA) have been extensively investigated for clinical applications.
30068880	2	37	theme	delivery	260:267	arg1	available					296:304	available	296:304	available	296:304	Among the various nucleic acid delivery systems that are currently available, lipid-nanoparticles (LNPs) that contain short interfering RNA (siRNA) have been extensively investigated for clinical applications.
30068880	4	38	used	used	607:610	arg2	they					595:598	they	595:598	they	595:598	In this method, it is necessary to remove the alcohol and then concentrate the LNP sample before they can be used.
30068880	2	39	theme	nucleic	247:253	arg1	systems					269:275	the various nucleic acid delivery systems	235:275	the various nucleic acid delivery systems that are currently available	235:304	Among the various nucleic acid delivery systems that are currently available, lipid-nanoparticles (LNPs) that contain short interfering RNA (siRNA) have been extensively investigated for clinical applications.
30068880	2	39	theme	nucleic	247:253	arg1	available					296:304	available	296:304	available	296:304	Among the various nucleic acid delivery systems that are currently available, lipid-nanoparticles (LNPs) that contain short interfering RNA (siRNA) have been extensively investigated for clinical applications.
30068880	2	40	theme	acid	255:258	arg1	systems					269:275	the various nucleic acid delivery systems	235:275	the various nucleic acid delivery systems that are currently available	235:304	Among the various nucleic acid delivery systems that are currently available, lipid-nanoparticles (LNPs) that contain short interfering RNA (siRNA) have been extensively investigated for clinical applications.
30068880	2	40	theme	acid	255:258	arg1	available					296:304	available	296:304	available	296:304	Among the various nucleic acid delivery systems that are currently available, lipid-nanoparticles (LNPs) that contain short interfering RNA (siRNA) have been extensively investigated for clinical applications.
30068880	1	41	theme	nucleic	145:151	arg1	acids					153:157	nucleic acids	145:157	nucleic acids	145:157	Systems for delivering nucleic acids are now fundamental technologies for realizing personalized medicine.
30068880	9	42	theme	conventional	1382:1393	arg1	method					1411:1416	the conventional ultrafiltration method	1378:1416	the conventional ultrafiltration method	1378:1416	The knockdown efficiency of the LNP prepared by the alcohol dilution-lyophilization method was comparable to that of an LNP prepared by the conventional ultrafiltration method.
30068880	5	43	theme	dilution-lyophilization	672:694	arg1	"					702:702	an "alcohol dilution-lyophilization method"	660:702	an "alcohol dilution-lyophilization method" for preparing siRNA-encapsulating LNPs	660:741	In this study, we report on the development of an "alcohol dilution-lyophilization method" for preparing siRNA-encapsulating LNPs.
30068880	2	44	theme	various	239:245	arg1	systems					269:275	the various nucleic acid delivery systems	235:275	the various nucleic acid delivery systems that are currently available	235:304	Among the various nucleic acid delivery systems that are currently available, lipid-nanoparticles (LNPs) that contain short interfering RNA (siRNA) have been extensively investigated for clinical applications.
30068880	2	44	theme	various	239:245	arg1	available					296:304	available	296:304	available	296:304	Among the various nucleic acid delivery systems that are currently available, lipid-nanoparticles (LNPs) that contain short interfering RNA (siRNA) have been extensively investigated for clinical applications.
30068880	9	45	theme	ultrafiltration	1395:1409	arg1	method					1411:1416	the conventional ultrafiltration method	1378:1416	the conventional ultrafiltration method	1378:1416	The knockdown efficiency of the LNP prepared by the alcohol dilution-lyophilization method was comparable to that of an LNP prepared by the conventional ultrafiltration method.
30068880	5	46	theme	method	696:701	arg1	"					702:702	an "alcohol dilution-lyophilization method"	660:702	an "alcohol dilution-lyophilization method" for preparing siRNA-encapsulating LNPs	660:741	In this study, we report on the development of an "alcohol dilution-lyophilization method" for preparing siRNA-encapsulating LNPs.
30068880	6	47	theme	residual	830:837	arg1	alcohol					839:845	the residual alcohol	826:845	the residual alcohol	826:845	This method involves the use of a freeze-drying (lyophilization) method to remove the residual alcohol and to simultaneously concentrate the preparation.
30068880	7	48	used	used	991:994	arg2	compositions					912:923	the compositions	908:923	the compositions of cryoprotectants and polyethylene glycol (PEG)-lipids that were used	908:994	At first, the compositions of cryoprotectants and polyethylene glycol (PEG)-lipids that were used were optimized from the point of view of particle stabilization.
30068880	3	49	theme	dilution	481:488	arg1	method					490:495	an alcohol dilution method	470:495	an alcohol dilution method	470:495	LNPs are generally prepared by an alcohol dilution method.
30068880	0	50	theme	Lipid	71:75	arg1	Nanoparticles					77:89	Lipid Nanoparticles	71:89	Lipid Nanoparticles	71:89	Development of an Alcohol Dilution-Lyophilization Method for Preparing Lipid Nanoparticles Containing Encapsulated siRNA.
30068880	1	51	theme	fundamental	167:177	arg1	Systems					122:128	Systems	122:128	Systems for delivering nucleic acids	122:157	Systems for delivering nucleic acids are now fundamental technologies for realizing personalized medicine.
30068880	1	51	theme	fundamental	167:177	arg1	technologies					179:190	fundamental technologies	167:190	fundamental technologies for realizing personalized medicine	167:226	Systems for delivering nucleic acids are now fundamental technologies for realizing personalized medicine.
30248951	4	0	dep	spectroscopy	851:862	arg1	spectra					900:906	spectra	900:906	spectra	900:906	Scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FT-IR) and X-ray diffraction (XRD) spectra indicated the successful synthesis of high-density AgNPs on the surface of PDA-SS/Agar film.
30248951	5	1	theme	sericin	1068:1074	arg1	structure					1055:1063	the structure	1051:1063	the structure of sericin and agar	1051:1083	PDA coating and AgNPs modification did not affect the structure of sericin and agar.
30248951	2	2	theme	ion	367:369	arg1	binding					371:377	silver ion binding	360:377	silver ion binding	360:377	Polydopamine (PDA) has great advantages for synthesizing large amounts of AgNPs, as it has multiple sites for silver ion binding and phenolic hydroxyl structure to reduce silver ions to AgNPs.
30248951	4	3	theme	film	995:998	arg1	surface					972:978	the surface	968:978	the surface of PDA-SS/Agar film	968:998	Scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FT-IR) and X-ray diffraction (XRD) spectra indicated the successful synthesis of high-density AgNPs on the surface of PDA-SS/Agar film.
30248951	2	4	contain	has	269:271	arg1	PDA					264:266	PDA	264:266	PDA	264:266	Polydopamine (PDA) has great advantages for synthesizing large amounts of AgNPs, as it has multiple sites for silver ion binding and phenolic hydroxyl structure to reduce silver ions to AgNPs.
30248951	2	4	contain	has	269:271	arg1	Polydopamine					250:261	Polydopamine	250:261	Polydopamine (PDA)	250:267	Polydopamine (PDA) has great advantages for synthesizing large amounts of AgNPs, as it has multiple sites for silver ion binding and phenolic hydroxyl structure to reduce silver ions to AgNPs.
30248951	2	4	contain	has	269:271	arg2	advantages					279:288	great advantages	273:288	great advantages for synthesizing large amounts of AgNPs	273:328	Polydopamine (PDA) has great advantages for synthesizing large amounts of AgNPs, as it has multiple sites for silver ion binding and phenolic hydroxyl structure to reduce silver ions to AgNPs.
30248951	0	5	theme	Dressing	97:104	arg1	Application					106:116	Potential Wound Dressing Application	81:116	Potential Wound Dressing Application	81:116	Polydopamine-Assisted Silver Nanoparticle Self-Assembly on Sericin/Agar Film for Potential Wound Dressing Application.
30248951	7	6	theme	excellent	1340:1348	arg1	ability					1381:1387	excellent and long-lasting antibacterial ability	1340:1387	excellent and long-lasting antibacterial ability	1340:1387	Inhibition zone and growth curve assays suggested the prepared film had excellent and long-lasting antibacterial ability.
30248951	6	7	theme	mechanical	1245:1254	arg1	properties					1256:1265	proper mechanical properties	1238:1265	proper mechanical properties	1238:1265	Furthermore, water contact angle, water absorption and mechanical property analysis showed that AgNPs-PDA-SS/Agar film had excellent hydrophilicity and proper mechanical properties.
30248951	2	8	theme	silver	360:365	arg1	binding					371:377	silver ion binding	360:377	silver ion binding	360:377	Polydopamine (PDA) has great advantages for synthesizing large amounts of AgNPs, as it has multiple sites for silver ion binding and phenolic hydroxyl structure to reduce silver ions to AgNPs.
30248951	3	9	theme	SS/Agar	627:633	arg1	film					635:638	SS/Agar film	627:638	SS/Agar film	627:638	Here, we mixed sericin and agar solution and dried at 65 °C to prepare a sericin (SS)/Agar composite film, and then coated polydopamine (PDA) on the surface of SS/Agar film by soaking SS/Agar film into polydopamine solution, subsequently synthesizing high-density AgNPs with the assistance of PDA to yield antibacterial AgNPs-PDA- SS/Agar film.
30248951	6	10	theme	proper	1238:1243	arg1	properties					1256:1265	proper mechanical properties	1238:1265	proper mechanical properties	1238:1265	Furthermore, water contact angle, water absorption and mechanical property analysis showed that AgNPs-PDA-SS/Agar film had excellent hydrophilicity and proper mechanical properties.
30248951	1	11	from	broad-spectrum	182:195	arg1	years					243:247	recent years	236:247	recent years	236:247	Silver nanoparticles (AgNPs) are extensively applied for their broad-spectrum and excellent antibacterial ability in recent years.
30248951	6	12	theme	water	1120:1124	arg1	absorption					1126:1135	water absorption	1120:1135	water absorption	1120:1135	Furthermore, water contact angle, water absorption and mechanical property analysis showed that AgNPs-PDA-SS/Agar film had excellent hydrophilicity and proper mechanical properties.
30248951	9	13	theme	dressing	1527:1534	arg1	potential					1492:1500	great potential	1486:1500	great potential	1486:1500	The film shows great potential as a novel kind of wound dressing.
30248951	9	13	theme	dressing	1527:1534	arg1	kind					1513:1516	a novel kind	1505:1516	a novel kind of wound dressing	1505:1534	The film shows great potential as a novel kind of wound dressing.
30248951	3	14	theme	SS/Agar	774:780	arg1	film					782:785	antibacterial AgNPs-PDA- SS/Agar film	749:785	antibacterial AgNPs-PDA- SS/Agar film	749:785	Here, we mixed sericin and agar solution and dried at 65 °C to prepare a sericin (SS)/Agar composite film, and then coated polydopamine (PDA) on the surface of SS/Agar film by soaking SS/Agar film into polydopamine solution, subsequently synthesizing high-density AgNPs with the assistance of PDA to yield antibacterial AgNPs-PDA- SS/Agar film.
30248951	6	15	theme	water	1099:1103	arg1	angle					1113:1117	water contact angle	1099:1117	water contact angle	1099:1117	Furthermore, water contact angle, water absorption and mechanical property analysis showed that AgNPs-PDA-SS/Agar film had excellent hydrophilicity and proper mechanical properties.
30248951	9	16	theme	wound	1521:1525	arg1	dressing					1527:1534	wound dressing	1521:1534	wound dressing	1521:1534	The film shows great potential as a novel kind of wound dressing.
30248951	6	17	theme	excellent	1209:1217	arg1	hydrophilicity					1219:1232	excellent hydrophilicity	1209:1232	excellent hydrophilicity	1209:1232	Furthermore, water contact angle, water absorption and mechanical property analysis showed that AgNPs-PDA-SS/Agar film had excellent hydrophilicity and proper mechanical properties.
30248951	7	18	theme	long-lasting	1354:1365	arg1	ability					1381:1387	excellent and long-lasting antibacterial ability	1340:1387	excellent and long-lasting antibacterial ability	1340:1387	Inhibition zone and growth curve assays suggested the prepared film had excellent and long-lasting antibacterial ability.
30248951	2	19	theme	large	307:311	arg1	amounts					313:319	large amounts	307:319	large amounts of AgNPs	307:328	Polydopamine (PDA) has great advantages for synthesizing large amounts of AgNPs, as it has multiple sites for silver ion binding and phenolic hydroxyl structure to reduce silver ions to AgNPs.
30248951	2	19	theme	large	307:311	arg1	AgNPs					324:328	AgNPs	324:328	AgNPs	324:328	Polydopamine (PDA) has great advantages for synthesizing large amounts of AgNPs, as it has multiple sites for silver ion binding and phenolic hydroxyl structure to reduce silver ions to AgNPs.
30248951	7	20	theme	growth	1288:1293	arg1	assays					1301:1306	Inhibition zone and growth curve assays	1268:1306	assays	1301:1306	Inhibition zone and growth curve assays suggested the prepared film had excellent and long-lasting antibacterial ability.
30248951	3	21	theme	polydopamine	645:656	arg1	solution					658:665	polydopamine solution	645:665	polydopamine solution	645:665	Here, we mixed sericin and agar solution and dried at 65 °C to prepare a sericin (SS)/Agar composite film, and then coated polydopamine (PDA) on the surface of SS/Agar film by soaking SS/Agar film into polydopamine solution, subsequently synthesizing high-density AgNPs with the assistance of PDA to yield antibacterial AgNPs-PDA- SS/Agar film.
30248951	4	22	theme	high-density	946:957	arg1	AgNPs					959:963	high-density AgNPs	946:963	high-density AgNPs	946:963	Scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FT-IR) and X-ray diffraction (XRD) spectra indicated the successful synthesis of high-density AgNPs on the surface of PDA-SS/Agar film.
30248951	2	23	dep	binding	371:377	arg1	structure					401:409	structure	401:409	structure	401:409	Polydopamine (PDA) has great advantages for synthesizing large amounts of AgNPs, as it has multiple sites for silver ion binding and phenolic hydroxyl structure to reduce silver ions to AgNPs.
30248951	1	24	theme	excellent	201:209	arg1	ability					225:231	their broad-spectrum and excellent antibacterial ability	176:231	ability	225:231	Silver nanoparticles (AgNPs) are extensively applied for their broad-spectrum and excellent antibacterial ability in recent years.
30248951	0	25	theme	Silver	22:27	arg1	Self-Assembly					42:54	Silver Nanoparticle Self-Assembly	22:54	Silver Nanoparticle Self-Assembly on Sericin/Agar Film for Potential Wound Dressing Application	22:116	Polydopamine-Assisted Silver Nanoparticle Self-Assembly on Sericin/Agar Film for Potential Wound Dressing Application.
30248951	4	26	theme	successful	922:931	arg1	synthesis					933:941	the successful synthesis	918:941	the successful synthesis of high-density AgNPs on the surface of PDA-SS/Agar film	918:998	Scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FT-IR) and X-ray diffraction (XRD) spectra indicated the successful synthesis of high-density AgNPs on the surface of PDA-SS/Agar film.
30248951	9	27	theme	novel	1507:1511	arg1	potential					1492:1500	great potential	1486:1500	great potential	1486:1500	The film shows great potential as a novel kind of wound dressing.
30248951	9	27	theme	novel	1507:1511	arg1	kind					1513:1516	a novel kind	1505:1516	a novel kind of wound dressing	1505:1534	The film shows great potential as a novel kind of wound dressing.
30248951	3	28	theme	/Agar	528:532	arg1	film					544:547	a sericin (SS)/Agar composite film	514:547	a sericin (SS)/Agar composite film	514:547	Here, we mixed sericin and agar solution and dried at 65 °C to prepare a sericin (SS)/Agar composite film, and then coated polydopamine (PDA) on the surface of SS/Agar film by soaking SS/Agar film into polydopamine solution, subsequently synthesizing high-density AgNPs with the assistance of PDA to yield antibacterial AgNPs-PDA- SS/Agar film.
30248951	1	29	theme	antibacterial	211:223	arg1	ability					225:231	their broad-spectrum and excellent antibacterial ability	176:231	ability	225:231	Silver nanoparticles (AgNPs) are extensively applied for their broad-spectrum and excellent antibacterial ability in recent years.
30248951	9	30	theme	great	1486:1490	arg1	potential					1492:1500	great potential	1486:1500	great potential	1486:1500	The film shows great potential as a novel kind of wound dressing.
30248951	9	30	theme	great	1486:1490	arg1	kind					1513:1516	a novel kind	1505:1516	a novel kind of wound dressing	1505:1534	The film shows great potential as a novel kind of wound dressing.
30248951	5	31	theme	agar	1080:1083	arg1	structure					1055:1063	the structure	1051:1063	the structure of sericin and agar	1051:1083	PDA coating and AgNPs modification did not affect the structure of sericin and agar.
30248951	1	32	theme	Silver	119:124	arg1	AgNPs					141:145	AgNPs	141:145	AgNPs	141:145	Silver nanoparticles (AgNPs) are extensively applied for their broad-spectrum and excellent antibacterial ability in recent years.
30248951	1	32	theme	Silver	119:124	arg1	nanoparticles					126:138	Silver nanoparticles	119:138	Silver nanoparticles (AgNPs)	119:146	Silver nanoparticles (AgNPs) are extensively applied for their broad-spectrum and excellent antibacterial ability in recent years.
30248951	2	33	theme	phenolic	383:390	arg1	hydroxyl					392:399	phenolic hydroxyl	383:399	phenolic hydroxyl	383:399	Polydopamine (PDA) has great advantages for synthesizing large amounts of AgNPs, as it has multiple sites for silver ion binding and phenolic hydroxyl structure to reduce silver ions to AgNPs.
30248951	4	34	theme	PDA-SS/Agar	983:993	arg1	film					995:998	PDA-SS/Agar film	983:998	PDA-SS/Agar film	983:998	Scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FT-IR) and X-ray diffraction (XRD) spectra indicated the successful synthesis of high-density AgNPs on the surface of PDA-SS/Agar film.
30248951	8	35	theme	excellent	1410:1418	arg1	cytocompatibility					1420:1436	excellent cytocompatibility	1410:1436	excellent cytocompatibility	1410:1436	In addition, it had excellent cytocompatibility on the fibroblast NIH/3T3 cells.
30248951	7	36	dep	suggested	1308:1316	arg1	had					1336:1338	had	1336:1338	suggested the prepared film had excellent and long-lasting antibacterial ability	1308:1387	Inhibition zone and growth curve assays suggested the prepared film had excellent and long-lasting antibacterial ability.
30248951	6	37	contain	had	1205:1207	arg2	properties					1256:1265	proper mechanical properties	1238:1265	proper mechanical properties	1238:1265	Furthermore, water contact angle, water absorption and mechanical property analysis showed that AgNPs-PDA-SS/Agar film had excellent hydrophilicity and proper mechanical properties.
30248951	6	37	contain	had	1205:1207	arg1	film					1200:1203	AgNPs-PDA-SS/Agar film	1182:1203	AgNPs-PDA-SS/Agar film	1182:1203	Furthermore, water contact angle, water absorption and mechanical property analysis showed that AgNPs-PDA-SS/Agar film had excellent hydrophilicity and proper mechanical properties.
30248951	6	37	contain	had	1205:1207	arg2	hydrophilicity					1219:1232	excellent hydrophilicity	1209:1232	excellent hydrophilicity	1209:1232	Furthermore, water contact angle, water absorption and mechanical property analysis showed that AgNPs-PDA-SS/Agar film had excellent hydrophilicity and proper mechanical properties.
30248951	0	38	theme	Nanoparticle	29:40	arg1	Self-Assembly					42:54	Silver Nanoparticle Self-Assembly	22:54	Silver Nanoparticle Self-Assembly on Sericin/Agar Film for Potential Wound Dressing Application	22:116	Polydopamine-Assisted Silver Nanoparticle Self-Assembly on Sericin/Agar Film for Potential Wound Dressing Application.
30248951	6	39	theme	angle	1113:1117	arg1	analysis					1161:1168	water contact angle, water absorption and mechanical property analysis	1099:1168	water contact angle, water absorption and mechanical property analysis	1099:1168	Furthermore, water contact angle, water absorption and mechanical property analysis showed that AgNPs-PDA-SS/Agar film had excellent hydrophilicity and proper mechanical properties.
30248951	2	40	theme	AgNPs	324:328	arg1	amounts					313:319	large amounts	307:319	large amounts of AgNPs	307:328	Polydopamine (PDA) has great advantages for synthesizing large amounts of AgNPs, as it has multiple sites for silver ion binding and phenolic hydroxyl structure to reduce silver ions to AgNPs.
30248951	2	40	theme	AgNPs	324:328	arg1	AgNPs					324:328	AgNPs	324:328	AgNPs	324:328	Polydopamine (PDA) has great advantages for synthesizing large amounts of AgNPs, as it has multiple sites for silver ion binding and phenolic hydroxyl structure to reduce silver ions to AgNPs.
30248951	1	41	theme	recent	236:241	arg1	years					243:247	recent years	236:247	recent years	236:247	Silver nanoparticles (AgNPs) are extensively applied for their broad-spectrum and excellent antibacterial ability in recent years.
30248951	0	42	theme	Sericin/Agar	59:70	arg1	Film					72:75	Sericin/Agar Film	59:75	Sericin/Agar Film for Potential Wound Dressing Application	59:116	Polydopamine-Assisted Silver Nanoparticle Self-Assembly on Sericin/Agar Film for Potential Wound Dressing Application.
30248951	3	43	theme	high-density	694:705	arg1	AgNPs					707:711	high-density AgNPs	694:711	high-density AgNPs	694:711	Here, we mixed sericin and agar solution and dried at 65 °C to prepare a sericin (SS)/Agar composite film, and then coated polydopamine (PDA) on the surface of SS/Agar film by soaking SS/Agar film into polydopamine solution, subsequently synthesizing high-density AgNPs with the assistance of PDA to yield antibacterial AgNPs-PDA- SS/Agar film.
30248951	4	44	dep	transform	832:840	arg1	infrared					842:849	infrared	842:849	transform infrared spectroscopy (FT-IR) and X-ray diffraction (XRD) spectra	832:906	Scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FT-IR) and X-ray diffraction (XRD) spectra indicated the successful synthesis of high-density AgNPs on the surface of PDA-SS/Agar film.
30248951	4	45	from	synthesis	933:941	arg1	surface					972:978	the surface	968:978	the surface of PDA-SS/Agar film	968:998	Scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FT-IR) and X-ray diffraction (XRD) spectra indicated the successful synthesis of high-density AgNPs on the surface of PDA-SS/Agar film.
30248951	6	46	theme	AgNPs-PDA-SS/Agar	1182:1198	arg1	film					1200:1203	AgNPs-PDA-SS/Agar film	1182:1203	AgNPs-PDA-SS/Agar film	1182:1203	Furthermore, water contact angle, water absorption and mechanical property analysis showed that AgNPs-PDA-SS/Agar film had excellent hydrophilicity and proper mechanical properties.
30248951	3	47	theme	agar	470:473	arg1	solution					475:482	agar solution	470:482	agar solution	470:482	Here, we mixed sericin and agar solution and dried at 65 °C to prepare a sericin (SS)/Agar composite film, and then coated polydopamine (PDA) on the surface of SS/Agar film by soaking SS/Agar film into polydopamine solution, subsequently synthesizing high-density AgNPs with the assistance of PDA to yield antibacterial AgNPs-PDA- SS/Agar film.
30248951	2	48	theme	silver	421:426	arg1	ions					428:431	silver ions	421:431	silver ions to AgNPs	421:440	Polydopamine (PDA) has great advantages for synthesizing large amounts of AgNPs, as it has multiple sites for silver ion binding and phenolic hydroxyl structure to reduce silver ions to AgNPs.
30248951	4	49	dep	Fourier	824:830	arg1	transform					832:840	transform	832:840	transform infrared spectroscopy (FT-IR) and X-ray diffraction (XRD) spectra	832:906	Scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FT-IR) and X-ray diffraction (XRD) spectra indicated the successful synthesis of high-density AgNPs on the surface of PDA-SS/Agar film.
30248951	4	50	theme	X-ray	876:880	arg1	XRD					895:897	XRD	895:897	XRD	895:897	Scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FT-IR) and X-ray diffraction (XRD) spectra indicated the successful synthesis of high-density AgNPs on the surface of PDA-SS/Agar film.
30248951	4	50	theme	X-ray	876:880	arg1	diffraction					882:892	X-ray diffraction	876:892	X-ray diffraction (XRD)	876:898	Scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FT-IR) and X-ray diffraction (XRD) spectra indicated the successful synthesis of high-density AgNPs on the surface of PDA-SS/Agar film.
30248951	8	51	theme	fibroblast	1445:1454	arg1	cells					1464:1468	the fibroblast NIH/3T3 cells	1441:1468	the fibroblast NIH/3T3 cells	1441:1468	In addition, it had excellent cytocompatibility on the fibroblast NIH/3T3 cells.
30248951	7	52	theme	antibacterial	1367:1379	arg1	ability					1381:1387	excellent and long-lasting antibacterial ability	1340:1387	excellent and long-lasting antibacterial ability	1340:1387	Inhibition zone and growth curve assays suggested the prepared film had excellent and long-lasting antibacterial ability.
30248951	1	53	from	years	243:247	arg1	broad-spectrum					182:195	broad-spectrum	182:195	broad-spectrum	182:195	Silver nanoparticles (AgNPs) are extensively applied for their broad-spectrum and excellent antibacterial ability in recent years.
30248951	7	54	theme	Inhibition	1268:1277	arg1	zone					1279:1282	Inhibition zone and growth curve assays	1268:1306	zone	1279:1282	Inhibition zone and growth curve assays suggested the prepared film had excellent and long-lasting antibacterial ability.
30248951	3	55	theme	PDA	736:738	arg1	assistance					722:731	the assistance	718:731	the assistance of PDA to yield antibacterial AgNPs-PDA- SS/Agar film	718:785	Here, we mixed sericin and agar solution and dried at 65 °C to prepare a sericin (SS)/Agar composite film, and then coated polydopamine (PDA) on the surface of SS/Agar film by soaking SS/Agar film into polydopamine solution, subsequently synthesizing high-density AgNPs with the assistance of PDA to yield antibacterial AgNPs-PDA- SS/Agar film.
30248951	4	56	theme	Scanning	788:795	arg1	SEM					818:820	SEM	818:820	SEM	818:820	Scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FT-IR) and X-ray diffraction (XRD) spectra indicated the successful synthesis of high-density AgNPs on the surface of PDA-SS/Agar film.
30248951	4	56	theme	Scanning	788:795	arg1	microscope					806:815	Scanning electron microscope	788:815	Scanning electron microscope (SEM)	788:821	Scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FT-IR) and X-ray diffraction (XRD) spectra indicated the successful synthesis of high-density AgNPs on the surface of PDA-SS/Agar film.
30248951	4	57	theme	AgNPs	959:963	arg1	synthesis					933:941	the successful synthesis	918:941	the successful synthesis of high-density AgNPs on the surface of PDA-SS/Agar film	918:998	Scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FT-IR) and X-ray diffraction (XRD) spectra indicated the successful synthesis of high-density AgNPs on the surface of PDA-SS/Agar film.
30248951	2	58	contain	has	337:339	arg2	sites					350:354	multiple sites	341:354	multiple sites for silver ion binding and phenolic hydroxyl structure	341:409	Polydopamine (PDA) has great advantages for synthesizing large amounts of AgNPs, as it has multiple sites for silver ion binding and phenolic hydroxyl structure to reduce silver ions to AgNPs.
30248951	2	58	contain	has	337:339	arg1	it					334:335	it	334:335	it	334:335	Polydopamine (PDA) has great advantages for synthesizing large amounts of AgNPs, as it has multiple sites for silver ion binding and phenolic hydroxyl structure to reduce silver ions to AgNPs.
30248951	3	59	theme	film	611:614	arg1	surface					592:598	the surface	588:598	the surface of SS/Agar film	588:614	Here, we mixed sericin and agar solution and dried at 65 °C to prepare a sericin (SS)/Agar composite film, and then coated polydopamine (PDA) on the surface of SS/Agar film by soaking SS/Agar film into polydopamine solution, subsequently synthesizing high-density AgNPs with the assistance of PDA to yield antibacterial AgNPs-PDA- SS/Agar film.
30248951	4	60	theme	electron	797:804	arg1	SEM					818:820	SEM	818:820	SEM	818:820	Scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FT-IR) and X-ray diffraction (XRD) spectra indicated the successful synthesis of high-density AgNPs on the surface of PDA-SS/Agar film.
30248951	4	60	theme	electron	797:804	arg1	microscope					806:815	Scanning electron microscope	788:815	Scanning electron microscope (SEM)	788:821	Scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FT-IR) and X-ray diffraction (XRD) spectra indicated the successful synthesis of high-density AgNPs on the surface of PDA-SS/Agar film.
30248951	7	61	theme	prepared	1322:1329	arg1	film					1331:1334	the prepared film	1318:1334	the prepared film	1318:1334	Inhibition zone and growth curve assays suggested the prepared film had excellent and long-lasting antibacterial ability.
30248951	8	62	theme	NIH/3T3	1456:1462	arg1	cells					1464:1468	the fibroblast NIH/3T3 cells	1441:1468	the fibroblast NIH/3T3 cells	1441:1468	In addition, it had excellent cytocompatibility on the fibroblast NIH/3T3 cells.
30248951	6	63	theme	property	1152:1159	arg1	analysis					1161:1168	water contact angle, water absorption and mechanical property analysis	1099:1168	water contact angle, water absorption and mechanical property analysis	1099:1168	Furthermore, water contact angle, water absorption and mechanical property analysis showed that AgNPs-PDA-SS/Agar film had excellent hydrophilicity and proper mechanical properties.
30248951	2	64	theme	multiple	341:348	arg1	sites					350:354	multiple sites	341:354	multiple sites for silver ion binding and phenolic hydroxyl structure	341:409	Polydopamine (PDA) has great advantages for synthesizing large amounts of AgNPs, as it has multiple sites for silver ion binding and phenolic hydroxyl structure to reduce silver ions to AgNPs.
30248951	3	65	theme	antibacterial	749:761	arg1	film					782:785	antibacterial AgNPs-PDA- SS/Agar film	749:785	antibacterial AgNPs-PDA- SS/Agar film	749:785	Here, we mixed sericin and agar solution and dried at 65 °C to prepare a sericin (SS)/Agar composite film, and then coated polydopamine (PDA) on the surface of SS/Agar film by soaking SS/Agar film into polydopamine solution, subsequently synthesizing high-density AgNPs with the assistance of PDA to yield antibacterial AgNPs-PDA- SS/Agar film.
30248951	6	66	theme	contact	1105:1111	arg1	angle					1113:1117	water contact angle	1099:1117	water contact angle	1099:1117	Furthermore, water contact angle, water absorption and mechanical property analysis showed that AgNPs-PDA-SS/Agar film had excellent hydrophilicity and proper mechanical properties.
30248951	5	67	theme	PDA	1001:1003	arg1	coating					1005:1011	PDA coating	1001:1011	PDA coating	1001:1011	PDA coating and AgNPs modification did not affect the structure of sericin and agar.
30248951	6	68	theme	mechanical	1141:1150	arg1	property					1152:1159	mechanical property	1141:1159	mechanical property	1141:1159	Furthermore, water contact angle, water absorption and mechanical property analysis showed that AgNPs-PDA-SS/Agar film had excellent hydrophilicity and proper mechanical properties.
30248951	5	69	theme	AgNPs	1017:1021	arg1	modification					1023:1034	AgNPs modification	1017:1034	AgNPs modification	1017:1034	PDA coating and AgNPs modification did not affect the structure of sericin and agar.
30248951	7	70	theme	curve	1295:1299	arg1	assays					1301:1306	Inhibition zone and growth curve assays	1268:1306	assays	1301:1306	Inhibition zone and growth curve assays suggested the prepared film had excellent and long-lasting antibacterial ability.
30248951	3	71	theme	AgNPs-PDA-	763:772	arg1	film					782:785	antibacterial AgNPs-PDA- SS/Agar film	749:785	antibacterial AgNPs-PDA- SS/Agar film	749:785	Here, we mixed sericin and agar solution and dried at 65 °C to prepare a sericin (SS)/Agar composite film, and then coated polydopamine (PDA) on the surface of SS/Agar film by soaking SS/Agar film into polydopamine solution, subsequently synthesizing high-density AgNPs with the assistance of PDA to yield antibacterial AgNPs-PDA- SS/Agar film.
30248951	0	72	theme	Wound	91:95	arg1	Application					106:116	Potential Wound Dressing Application	81:116	Potential Wound Dressing Application	81:116	Polydopamine-Assisted Silver Nanoparticle Self-Assembly on Sericin/Agar Film for Potential Wound Dressing Application.
30248951	3	73	theme	SS/Agar	603:609	arg1	film					611:614	SS/Agar film	603:614	SS/Agar film	603:614	Here, we mixed sericin and agar solution and dried at 65 °C to prepare a sericin (SS)/Agar composite film, and then coated polydopamine (PDA) on the surface of SS/Agar film by soaking SS/Agar film into polydopamine solution, subsequently synthesizing high-density AgNPs with the assistance of PDA to yield antibacterial AgNPs-PDA- SS/Agar film.
30248951	2	74	theme	great	273:277	arg1	advantages					279:288	great advantages	273:288	great advantages for synthesizing large amounts of AgNPs	273:328	Polydopamine (PDA) has great advantages for synthesizing large amounts of AgNPs, as it has multiple sites for silver ion binding and phenolic hydroxyl structure to reduce silver ions to AgNPs.
30248951	8	75	contain	had	1406:1408	arg1	it					1403:1404	it	1403:1404	it	1403:1404	In addition, it had excellent cytocompatibility on the fibroblast NIH/3T3 cells.
30248951	8	75	contain	had	1406:1408	arg2	cytocompatibility					1420:1436	excellent cytocompatibility	1410:1436	excellent cytocompatibility	1410:1436	In addition, it had excellent cytocompatibility on the fibroblast NIH/3T3 cells.
30248951	0	76	theme	Potential	81:89	arg1	Application					106:116	Potential Wound Dressing Application	81:116	Potential Wound Dressing Application	81:116	Polydopamine-Assisted Silver Nanoparticle Self-Assembly on Sericin/Agar Film for Potential Wound Dressing Application.
30248951	0	77	from	Self-Assembly	42:54	arg1	Film					72:75	Sericin/Agar Film	59:75	Sericin/Agar Film for Potential Wound Dressing Application	59:116	Polydopamine-Assisted Silver Nanoparticle Self-Assembly on Sericin/Agar Film for Potential Wound Dressing Application.
30248951	6	78	theme	absorption	1126:1135	arg1	analysis					1161:1168	water contact angle, water absorption and mechanical property analysis	1099:1168	water contact angle, water absorption and mechanical property analysis	1099:1168	Furthermore, water contact angle, water absorption and mechanical property analysis showed that AgNPs-PDA-SS/Agar film had excellent hydrophilicity and proper mechanical properties.
30248951	3	79	theme	composite	534:542	arg1	film					544:547	a sericin (SS)/Agar composite film	514:547	a sericin (SS)/Agar composite film	514:547	Here, we mixed sericin and agar solution and dried at 65 °C to prepare a sericin (SS)/Agar composite film, and then coated polydopamine (PDA) on the surface of SS/Agar film by soaking SS/Agar film into polydopamine solution, subsequently synthesizing high-density AgNPs with the assistance of PDA to yield antibacterial AgNPs-PDA- SS/Agar film.
32624876	11	0	theme	activity	1469:1476	arg1	values					1443:1448	the highest values	1431:1448	the highest values of α-galactosidase activity	1431:1476	L. plantarum S27 showed the highest values of α-galactosidase activity at the 24-h fermentation process when it was cultivated in a broth with the presence of P. major hydrolysates with hemicellulase and xylanase (0.129 U/mg and 0.133 U/mg, respectively).
32624876	7	1	from	utilization	818:828	arg1	hydrolysates					876:887	the obtained hydrolysates	863:887	the obtained hydrolysates	863:887	The utilization of the carbohydrate complex from the obtained hydrolysates to stimulate the production of certain glycohydrolases from Lactobacillus plantarum probiotic strains was investigated.
32624876	8	2	theme	products	1045:1052	arg1	analysis					1018:1025	The HPLC analysis	1009:1025	The HPLC analysis of the hydrolysis products	1009:1052	The HPLC analysis of the hydrolysis products showed the presence of galactose and arabinose in 1:3 and 1:4 ratios.
32624876	10	3	theme	media	1329:1333	arg1	hydrolysates					1335:1346	P. media hydrolysates	1326:1346	P. media hydrolysates	1326:1346	The antioxidant activity according to the three methods was the highest in P. media hydrolysates, which correlated with their total phenolic concentration.
32624876	7	4	theme	probiotic	973:981	arg1	strains					983:989	Lactobacillus plantarum probiotic strains	949:989	Lactobacillus plantarum probiotic strains	949:989	The utilization of the carbohydrate complex from the obtained hydrolysates to stimulate the production of certain glycohydrolases from Lactobacillus plantarum probiotic strains was investigated.
32624876	9	5	theme	total	1153:1157	arg1	sugars					1159:1164	total sugars	1153:1164	total sugars	1153:1164	The highest concentration of total sugars was determined in P. major hydrolysates with hemicellulase (386.71 mg/g dry weight).
32624876	11	6	theme	P. major	1566:1573	arg1	hydrolysates					1575:1586	P. major hydrolysates	1566:1586	P. major hydrolysates	1566:1586	L. plantarum S27 showed the highest values of α-galactosidase activity at the 24-h fermentation process when it was cultivated in a broth with the presence of P. major hydrolysates with hemicellulase and xylanase (0.129 U/mg and 0.133 U/mg, respectively).
32624876	4	7	dep	Plantago	448:455	arg1	media					498:502	media	498:502	media	498:502	In this study enzymatic hydrolysis of Plantago major, Plantago lanceolata, and Plantago media leaves with hemicellulase and xylanase was carried out.
32624876	3	8	theme	compositions	336:347	arg1	influence					310:318	the relative influence	297:318	the relative influence of carbohydrate compositions and polyphenols on their antioxidant and prebiotic activity	297:407	In the recent years, some authors have examined the relative influence of carbohydrate compositions and polyphenols on their antioxidant and prebiotic activity.
32624876	0	9	from	Study	0:4	arg1	Plantago					57:64	Plantago	57:64	Plantago	57:64	Study of prebiotic potential and antioxidant activity in Plantago spp.
32624876	5	10	theme	enzyme-derived	592:605	arg1	products					618:625	the enzyme-derived hydrolysis products	588:625	the enzyme-derived hydrolysis products	588:625	The chemical composition of the enzyme-derived hydrolysis products was determined.
32624876	7	11	theme	Lactobacillus	949:961	arg1	strains					983:989	Lactobacillus plantarum probiotic strains	949:989	Lactobacillus plantarum probiotic strains	949:989	The utilization of the carbohydrate complex from the obtained hydrolysates to stimulate the production of certain glycohydrolases from Lactobacillus plantarum probiotic strains was investigated.
32624876	8	12	theme	hydrolysis	1034:1043	arg1	products					1045:1052	the hydrolysis products	1030:1052	the hydrolysis products	1030:1052	The HPLC analysis of the hydrolysis products showed the presence of galactose and arabinose in 1:3 and 1:4 ratios.
32624876	2	13	theme	Plantago	152:159	arg1	species					161:167	Plantago species	152:167	Plantago species	152:167	The leaves of Plantago species have been known as a rich source of polysaccharides, polyphenols, and iridoids.
32624876	5	14	theme	hydrolysis	607:616	arg1	products					618:625	the enzyme-derived hydrolysis products	588:625	the enzyme-derived hydrolysis products	588:625	The chemical composition of the enzyme-derived hydrolysis products was determined.
32624876	9	15	theme	sugars	1159:1164	arg1	concentration					1136:1148	The highest concentration	1124:1148	The highest concentration of total sugars	1124:1164	The highest concentration of total sugars was determined in P. major hydrolysates with hemicellulase (386.71 mg/g dry weight).
32624876	7	16	theme	certain	920:926	arg1	glycohydrolases					928:942	certain glycohydrolases	920:942	certain glycohydrolases	920:942	The utilization of the carbohydrate complex from the obtained hydrolysates to stimulate the production of certain glycohydrolases from Lactobacillus plantarum probiotic strains was investigated.
32624876	5	17	link	enzyme-derived	592:605	arg1	products					618:625	the enzyme-derived hydrolysis products	588:625	the enzyme-derived hydrolysis products	588:625	The chemical composition of the enzyme-derived hydrolysis products was determined.
32624876	11	18	theme	fermentation	1490:1501	arg1	process					1503:1509	the 24-h fermentation process	1481:1509	the 24-h fermentation process	1481:1509	L. plantarum S27 showed the highest values of α-galactosidase activity at the 24-h fermentation process when it was cultivated in a broth with the presence of P. major hydrolysates with hemicellulase and xylanase (0.129 U/mg and 0.133 U/mg, respectively).
32624876	8	19	dep	1:3	1104:1106	arg1	ratios					1116:1121	ratios	1116:1121	ratios	1116:1121	The HPLC analysis of the hydrolysis products showed the presence of galactose and arabinose in 1:3 and 1:4 ratios.
32624876	10	20	theme	total	1377:1381	arg1	concentration					1392:1404	their total phenolic concentration	1371:1404	their total phenolic concentration	1371:1404	The antioxidant activity according to the three methods was the highest in P. media hydrolysates, which correlated with their total phenolic concentration.
32624876	5	21	theme	products	618:625	arg1	composition					573:583	The chemical composition	560:583	The chemical composition of the enzyme-derived hydrolysis products	560:625	The chemical composition of the enzyme-derived hydrolysis products was determined.
32624876	6	22	theme	power	758:762	arg1	assays					806:811	2,2-diphenyl-2-picryl-hydrazyl-hydrate, ferric reducing antioxidant power, and cupric reducing antioxidant capacity assays	690:811	2,2-diphenyl-2-picryl-hydrazyl-hydrate, ferric reducing antioxidant power, and cupric reducing antioxidant capacity assays	690:811	Their antioxidant activity was evaluated using 2,2-diphenyl-2-picryl-hydrazyl-hydrate, ferric reducing antioxidant power, and cupric reducing antioxidant capacity assays.
32624876	3	23	theme	carbohydrate	323:334	arg1	compositions					336:347	carbohydrate compositions	323:347	carbohydrate compositions	323:347	In the recent years, some authors have examined the relative influence of carbohydrate compositions and polyphenols on their antioxidant and prebiotic activity.
32624876	11	24	theme	L.	1407:1408	arg1	S27					1420:1422	L. plantarum S27	1407:1422	L. plantarum S27	1407:1422	L. plantarum S27 showed the highest values of α-galactosidase activity at the 24-h fermentation process when it was cultivated in a broth with the presence of P. major hydrolysates with hemicellulase and xylanase (0.129 U/mg and 0.133 U/mg, respectively).
32624876	8	25	theme	arabinose	1091:1099	arg1	presence					1065:1072	the presence	1061:1072	the presence of galactose and arabinose in 1:3 and 1:4 ratios	1061:1121	The HPLC analysis of the hydrolysis products showed the presence of galactose and arabinose in 1:3 and 1:4 ratios.
32624876	6	26	theme	antioxidant	746:756	arg1	power					758:762	ferric reducing antioxidant power	730:762	ferric reducing antioxidant power	730:762	Their antioxidant activity was evaluated using 2,2-diphenyl-2-picryl-hydrazyl-hydrate, ferric reducing antioxidant power, and cupric reducing antioxidant capacity assays.
32624876	1	27	with	hydrolysis	94:103	arg1	xylanase					128:135	xylanase	128:135	xylanase	128:135	leaves after enzymatic hydrolysis with hemicellulase and xylanase.
32624876	1	27	with	hydrolysis	94:103	arg1	hemicellulase					110:122	hemicellulase	110:122	hemicellulase	110:122	leaves after enzymatic hydrolysis with hemicellulase and xylanase.
32624876	4	28	theme	lanceolata	473:482	arg1	hydrolysis					434:443	enzymatic hydrolysis	424:443	enzymatic hydrolysis of Plantago major, Plantago lanceolata, and Plantago media	424:502	In this study enzymatic hydrolysis of Plantago major, Plantago lanceolata, and Plantago media leaves with hemicellulase and xylanase was carried out.
32624876	5	29	theme	chemical	564:571	arg1	composition					573:583	The chemical composition	560:583	The chemical composition of the enzyme-derived hydrolysis products	560:625	The chemical composition of the enzyme-derived hydrolysis products was determined.
32624876	7	30	from	strains	983:989	arg1	production					906:915	the production	902:915	the production of certain glycohydrolases from Lactobacillus plantarum probiotic strains	902:989	The utilization of the carbohydrate complex from the obtained hydrolysates to stimulate the production of certain glycohydrolases from Lactobacillus plantarum probiotic strains was investigated.
32624876	6	31	theme	reducing	737:744	arg1	power					758:762	ferric reducing antioxidant power	730:762	ferric reducing antioxidant power	730:762	Their antioxidant activity was evaluated using 2,2-diphenyl-2-picryl-hydrazyl-hydrate, ferric reducing antioxidant power, and cupric reducing antioxidant capacity assays.
32624876	9	32	theme	highest	1128:1134	arg1	concentration					1136:1148	The highest concentration	1124:1148	The highest concentration of total sugars	1124:1164	The highest concentration of total sugars was determined in P. major hydrolysates with hemicellulase (386.71 mg/g dry weight).
32624876	3	33	theme	antioxidant	374:384	arg1	activity					400:407	their antioxidant and prebiotic activity	368:407	their antioxidant and prebiotic activity	368:407	In the recent years, some authors have examined the relative influence of carbohydrate compositions and polyphenols on their antioxidant and prebiotic activity.
32624876	7	34	theme	obtained	867:874	arg1	hydrolysates					876:887	the obtained hydrolysates	863:887	the obtained hydrolysates	863:887	The utilization of the carbohydrate complex from the obtained hydrolysates to stimulate the production of certain glycohydrolases from Lactobacillus plantarum probiotic strains was investigated.
32624876	11	35	dep	xylanase	1611:1618	arg1	U/mg					1627:1630	0.129 U/mg and 0.133 U/mg	1621:1645	U/mg	1627:1630	L. plantarum S27 showed the highest values of α-galactosidase activity at the 24-h fermentation process when it was cultivated in a broth with the presence of P. major hydrolysates with hemicellulase and xylanase (0.129 U/mg and 0.133 U/mg, respectively).
32624876	11	35	dep	xylanase	1611:1618	arg1	U/mg					1642:1645	0.129 U/mg and 0.133 U/mg	1621:1645	U/mg	1642:1645	L. plantarum S27 showed the highest values of α-galactosidase activity at the 24-h fermentation process when it was cultivated in a broth with the presence of P. major hydrolysates with hemicellulase and xylanase (0.129 U/mg and 0.133 U/mg, respectively).
32624876	1	36	theme	enzymatic	84:92	arg1	hydrolysis					94:103	enzymatic hydrolysis	84:103	enzymatic hydrolysis with hemicellulase and xylanase	84:135	leaves after enzymatic hydrolysis with hemicellulase and xylanase.
32624876	0	37	theme	activity	45:52	arg1	Study					0:4	Study	0:4	Study of prebiotic potential and antioxidant activity in Plantago	0:64	Study of prebiotic potential and antioxidant activity in Plantago spp.
32624876	3	38	theme	polyphenols	353:363	arg1	influence					310:318	the relative influence	297:318	the relative influence of carbohydrate compositions and polyphenols on their antioxidant and prebiotic activity	297:407	In the recent years, some authors have examined the relative influence of carbohydrate compositions and polyphenols on their antioxidant and prebiotic activity.
32624876	2	39	dep	The	138:140	arg1	leaves					142:147	leaves	142:147	leaves	142:147	The leaves of Plantago species have been known as a rich source of polysaccharides, polyphenols, and iridoids.
32624876	3	40	theme	recent	256:261	arg1	years					263:267	the recent years	252:267	the recent years	252:267	In the recent years, some authors have examined the relative influence of carbohydrate compositions and polyphenols on their antioxidant and prebiotic activity.
32624876	7	41	from	hydrolysates	876:887	arg1	utilization					818:828	The utilization	814:828	The utilization of the carbohydrate complex from the obtained hydrolysates to stimulate the production of certain glycohydrolases from Lactobacillus plantarum probiotic strains	814:989	The utilization of the carbohydrate complex from the obtained hydrolysates to stimulate the production of certain glycohydrolases from Lactobacillus plantarum probiotic strains was investigated.
32624876	7	41	from	hydrolysates	876:887	arg1	complex					850:856	the carbohydrate complex	833:856	the carbohydrate complex from the obtained hydrolysates to stimulate the production of certain glycohydrolases from Lactobacillus plantarum probiotic strains	833:989	The utilization of the carbohydrate complex from the obtained hydrolysates to stimulate the production of certain glycohydrolases from Lactobacillus plantarum probiotic strains was investigated.
32624876	11	42	theme	plantarum	1410:1418	arg1	S27					1420:1422	L. plantarum S27	1407:1422	L. plantarum S27	1407:1422	L. plantarum S27 showed the highest values of α-galactosidase activity at the 24-h fermentation process when it was cultivated in a broth with the presence of P. major hydrolysates with hemicellulase and xylanase (0.129 U/mg and 0.133 U/mg, respectively).
32624876	0	43	theme	prebiotic	9:17	arg1	potential					19:27	prebiotic potential	9:27	prebiotic potential	9:27	Study of prebiotic potential and antioxidant activity in Plantago spp.
32624876	7	44	theme	complex	850:856	arg1	utilization					818:828	The utilization	814:828	The utilization of the carbohydrate complex from the obtained hydrolysates to stimulate the production of certain glycohydrolases from Lactobacillus plantarum probiotic strains	814:989	The utilization of the carbohydrate complex from the obtained hydrolysates to stimulate the production of certain glycohydrolases from Lactobacillus plantarum probiotic strains was investigated.
32624876	11	45	with	broth	1539:1543	arg1	xylanase					1611:1618	xylanase	1611:1618	xylanase (0.129 U/mg and 0.133 U/mg, respectively)	1611:1660	L. plantarum S27 showed the highest values of α-galactosidase activity at the 24-h fermentation process when it was cultivated in a broth with the presence of P. major hydrolysates with hemicellulase and xylanase (0.129 U/mg and 0.133 U/mg, respectively).
32624876	11	45	with	broth	1539:1543	arg1	hemicellulase					1593:1605	hemicellulase	1593:1605	hemicellulase	1593:1605	L. plantarum S27 showed the highest values of α-galactosidase activity at the 24-h fermentation process when it was cultivated in a broth with the presence of P. major hydrolysates with hemicellulase and xylanase (0.129 U/mg and 0.133 U/mg, respectively).
32624876	11	45	with	broth	1539:1543	arg1	presence					1554:1561	the presence	1550:1561	the presence of P. major hydrolysates	1550:1586	L. plantarum S27 showed the highest values of α-galactosidase activity at the 24-h fermentation process when it was cultivated in a broth with the presence of P. major hydrolysates with hemicellulase and xylanase (0.129 U/mg and 0.133 U/mg, respectively).
32624876	6	46	theme	2,2-diphenyl-2-picryl-hydrazyl-hydrate	690:727	arg1	assays					806:811	2,2-diphenyl-2-picryl-hydrazyl-hydrate, ferric reducing antioxidant power, and cupric reducing antioxidant capacity assays	690:811	2,2-diphenyl-2-picryl-hydrazyl-hydrate, ferric reducing antioxidant power, and cupric reducing antioxidant capacity assays	690:811	Their antioxidant activity was evaluated using 2,2-diphenyl-2-picryl-hydrazyl-hydrate, ferric reducing antioxidant power, and cupric reducing antioxidant capacity assays.
32624876	6	47	theme	ferric	730:735	arg1	power					758:762	ferric reducing antioxidant power	730:762	ferric reducing antioxidant power	730:762	Their antioxidant activity was evaluated using 2,2-diphenyl-2-picryl-hydrazyl-hydrate, ferric reducing antioxidant power, and cupric reducing antioxidant capacity assays.
32624876	0	48	theme	antioxidant	33:43	arg1	activity					45:52	antioxidant activity	33:52	antioxidant activity	33:52	Study of prebiotic potential and antioxidant activity in Plantago spp.
32624876	7	49	theme	glycohydrolases	928:942	arg1	production					906:915	the production	902:915	the production of certain glycohydrolases from Lactobacillus plantarum probiotic strains	902:989	The utilization of the carbohydrate complex from the obtained hydrolysates to stimulate the production of certain glycohydrolases from Lactobacillus plantarum probiotic strains was investigated.
32624876	2	50	theme	polyphenols	222:232	arg1	The					138:140	The	138:140	The	138:140	The leaves of Plantago species have been known as a rich source of polysaccharides, polyphenols, and iridoids.
32624876	2	50	theme	polyphenols	222:232	arg1	source					195:200	a rich source	188:200	a rich source of polysaccharides, polyphenols, and iridoids	188:246	The leaves of Plantago species have been known as a rich source of polysaccharides, polyphenols, and iridoids.
32624876	10	51	theme	phenolic	1383:1390	arg1	concentration					1392:1404	their total phenolic concentration	1371:1404	their total phenolic concentration	1371:1404	The antioxidant activity according to the three methods was the highest in P. media hydrolysates, which correlated with their total phenolic concentration.
32624876	11	52	theme	α-galactosidase	1453:1467	arg1	activity					1469:1476	α-galactosidase activity	1453:1476	α-galactosidase activity	1453:1476	L. plantarum S27 showed the highest values of α-galactosidase activity at the 24-h fermentation process when it was cultivated in a broth with the presence of P. major hydrolysates with hemicellulase and xylanase (0.129 U/mg and 0.133 U/mg, respectively).
32624876	8	53	theme	galactose	1077:1085	arg1	presence					1065:1072	the presence	1061:1072	the presence of galactose and arabinose in 1:3 and 1:4 ratios	1061:1121	The HPLC analysis of the hydrolysis products showed the presence of galactose and arabinose in 1:3 and 1:4 ratios.
32624876	0	54	theme	potential	19:27	arg1	Study					0:4	Study	0:4	Study of prebiotic potential and antioxidant activity in Plantago	0:64	Study of prebiotic potential and antioxidant activity in Plantago spp.
32624876	8	55	theme	HPLC	1013:1016	arg1	analysis					1018:1025	The HPLC analysis	1009:1025	The HPLC analysis of the hydrolysis products	1009:1052	The HPLC analysis of the hydrolysis products showed the presence of galactose and arabinose in 1:3 and 1:4 ratios.
32624876	11	56	theme	highest	1435:1441	arg1	values					1443:1448	the highest values	1431:1448	the highest values of α-galactosidase activity	1431:1476	L. plantarum S27 showed the highest values of α-galactosidase activity at the 24-h fermentation process when it was cultivated in a broth with the presence of P. major hydrolysates with hemicellulase and xylanase (0.129 U/mg and 0.133 U/mg, respectively).
32624876	7	57	theme	carbohydrate	837:848	arg1	complex					850:856	the carbohydrate complex	833:856	the carbohydrate complex from the obtained hydrolysates to stimulate the production of certain glycohydrolases from Lactobacillus plantarum probiotic strains	833:989	The utilization of the carbohydrate complex from the obtained hydrolysates to stimulate the production of certain glycohydrolases from Lactobacillus plantarum probiotic strains was investigated.
32624876	4	58	theme	Plantago	448:455	arg1	hydrolysis					434:443	enzymatic hydrolysis	424:443	enzymatic hydrolysis of Plantago major, Plantago lanceolata, and Plantago media	424:502	In this study enzymatic hydrolysis of Plantago major, Plantago lanceolata, and Plantago media leaves with hemicellulase and xylanase was carried out.
32624876	2	59	theme	polysaccharides	205:219	arg1	The					138:140	The	138:140	The	138:140	The leaves of Plantago species have been known as a rich source of polysaccharides, polyphenols, and iridoids.
32624876	2	59	theme	polysaccharides	205:219	arg1	source					195:200	a rich source	188:200	a rich source of polysaccharides, polyphenols, and iridoids	188:246	The leaves of Plantago species have been known as a rich source of polysaccharides, polyphenols, and iridoids.
32624876	9	60	dep	determined	1170:1179	arg1	weight					1242:1247	386.71 mg/g dry weight	1226:1247	386.71 mg/g dry weight	1226:1247	The highest concentration of total sugars was determined in P. major hydrolysates with hemicellulase (386.71 mg/g dry weight).
32624876	3	61	theme	prebiotic	390:398	arg1	activity					400:407	their antioxidant and prebiotic activity	368:407	their antioxidant and prebiotic activity	368:407	In the recent years, some authors have examined the relative influence of carbohydrate compositions and polyphenols on their antioxidant and prebiotic activity.
32624876	11	62	theme	hydrolysates	1575:1586	arg1	presence					1554:1561	the presence	1550:1561	the presence of P. major hydrolysates	1550:1586	L. plantarum S27 showed the highest values of α-galactosidase activity at the 24-h fermentation process when it was cultivated in a broth with the presence of P. major hydrolysates with hemicellulase and xylanase (0.129 U/mg and 0.133 U/mg, respectively).
32624876	9	63	theme	386.71 mg/g	1226:1236	arg1	weight					1242:1247	386.71 mg/g dry weight	1226:1247	386.71 mg/g dry weight	1226:1247	The highest concentration of total sugars was determined in P. major hydrolysates with hemicellulase (386.71 mg/g dry weight).
32624876	10	64	theme	P.	1326:1327	arg1	hydrolysates					1335:1346	P. media hydrolysates	1326:1346	P. media hydrolysates	1326:1346	The antioxidant activity according to the three methods was the highest in P. media hydrolysates, which correlated with their total phenolic concentration.
32624876	2	65	theme	rich	190:193	arg1	The					138:140	The	138:140	The	138:140	The leaves of Plantago species have been known as a rich source of polysaccharides, polyphenols, and iridoids.
32624876	2	65	theme	rich	190:193	arg1	source					195:200	a rich source	188:200	a rich source of polysaccharides, polyphenols, and iridoids	188:246	The leaves of Plantago species have been known as a rich source of polysaccharides, polyphenols, and iridoids.
32624876	6	66	theme	capacity	797:804	arg1	assays					806:811	2,2-diphenyl-2-picryl-hydrazyl-hydrate, ferric reducing antioxidant power, and cupric reducing antioxidant capacity assays	690:811	2,2-diphenyl-2-picryl-hydrazyl-hydrate, ferric reducing antioxidant power, and cupric reducing antioxidant capacity assays	690:811	Their antioxidant activity was evaluated using 2,2-diphenyl-2-picryl-hydrazyl-hydrate, ferric reducing antioxidant power, and cupric reducing antioxidant capacity assays.
32624876	8	67	from	presence	1065:1072	arg1	1:4					1112:1114	1:4	1112:1114	1:4	1112:1114	The HPLC analysis of the hydrolysis products showed the presence of galactose and arabinose in 1:3 and 1:4 ratios.
32624876	8	67	from	presence	1065:1072	arg1	1:3					1104:1106	1:3	1104:1106	1:3	1104:1106	The HPLC analysis of the hydrolysis products showed the presence of galactose and arabinose in 1:3 and 1:4 ratios.
32624876	9	68	theme	dry	1238:1240	arg1	weight					1242:1247	386.71 mg/g dry weight	1226:1247	386.71 mg/g dry weight	1226:1247	The highest concentration of total sugars was determined in P. major hydrolysates with hemicellulase (386.71 mg/g dry weight).
32624876	4	69	theme	enzymatic	424:432	arg1	hydrolysis					434:443	enzymatic hydrolysis	424:443	enzymatic hydrolysis of Plantago major, Plantago lanceolata, and Plantago media	424:502	In this study enzymatic hydrolysis of Plantago major, Plantago lanceolata, and Plantago media leaves with hemicellulase and xylanase was carried out.
32624876	2	70	theme	iridoids	239:246	arg1	The					138:140	The	138:140	The	138:140	The leaves of Plantago species have been known as a rich source of polysaccharides, polyphenols, and iridoids.
32624876	2	70	theme	iridoids	239:246	arg1	source					195:200	a rich source	188:200	a rich source of polysaccharides, polyphenols, and iridoids	188:246	The leaves of Plantago species have been known as a rich source of polysaccharides, polyphenols, and iridoids.
32624876	6	71	theme	antioxidant	785:795	arg1	capacity					797:804	cupric reducing antioxidant capacity	769:804	cupric reducing antioxidant capacity	769:804	Their antioxidant activity was evaluated using 2,2-diphenyl-2-picryl-hydrazyl-hydrate, ferric reducing antioxidant power, and cupric reducing antioxidant capacity assays.
32624876	9	72	theme	P. major	1184:1191	arg1	hydrolysates					1193:1204	P. major hydrolysates	1184:1204	P. major hydrolysates	1184:1204	The highest concentration of total sugars was determined in P. major hydrolysates with hemicellulase (386.71 mg/g dry weight).
32624876	8	73	attach	presence	1065:1072	arg2	arabinose					1091:1099	arabinose	1091:1099	arabinose	1091:1099	The HPLC analysis of the hydrolysis products showed the presence of galactose and arabinose in 1:3 and 1:4 ratios.
32624876	8	73	attach	presence	1065:1072	arg2	galactose					1077:1085	galactose	1077:1085	galactose	1077:1085	The HPLC analysis of the hydrolysis products showed the presence of galactose and arabinose in 1:3 and 1:4 ratios.
32624876	8	73	attach	presence	1065:1072	arg1	1:4					1112:1114	1:4	1112:1114	1:4	1112:1114	The HPLC analysis of the hydrolysis products showed the presence of galactose and arabinose in 1:3 and 1:4 ratios.
32624876	8	73	attach	presence	1065:1072	arg1	1:3					1104:1106	1:3	1104:1106	1:3	1104:1106	The HPLC analysis of the hydrolysis products showed the presence of galactose and arabinose in 1:3 and 1:4 ratios.
32624876	3	74	theme	relative	301:308	arg1	influence					310:318	the relative influence	297:318	the relative influence of carbohydrate compositions and polyphenols on their antioxidant and prebiotic activity	297:407	In the recent years, some authors have examined the relative influence of carbohydrate compositions and polyphenols on their antioxidant and prebiotic activity.
32624876	3	75	from	influence	310:318	arg1	activity					400:407	their antioxidant and prebiotic activity	368:407	their antioxidant and prebiotic activity	368:407	In the recent years, some authors have examined the relative influence of carbohydrate compositions and polyphenols on their antioxidant and prebiotic activity.
32624876	6	76	theme	reducing	776:783	arg1	capacity					797:804	cupric reducing antioxidant capacity	769:804	cupric reducing antioxidant capacity	769:804	Their antioxidant activity was evaluated using 2,2-diphenyl-2-picryl-hydrazyl-hydrate, ferric reducing antioxidant power, and cupric reducing antioxidant capacity assays.
32624876	6	77	theme	antioxidant	649:659	arg1	activity					661:668	Their antioxidant activity	643:668	Their antioxidant activity	643:668	Their antioxidant activity was evaluated using 2,2-diphenyl-2-picryl-hydrazyl-hydrate, ferric reducing antioxidant power, and cupric reducing antioxidant capacity assays.
32624876	11	78	theme	24-h	1485:1488	arg1	process					1503:1509	the 24-h fermentation process	1481:1509	the 24-h fermentation process	1481:1509	L. plantarum S27 showed the highest values of α-galactosidase activity at the 24-h fermentation process when it was cultivated in a broth with the presence of P. major hydrolysates with hemicellulase and xylanase (0.129 U/mg and 0.133 U/mg, respectively).
32624876	4	79	theme	Plantago	489:496	arg1	hydrolysis					434:443	enzymatic hydrolysis	424:443	enzymatic hydrolysis of Plantago major, Plantago lanceolata, and Plantago media	424:502	In this study enzymatic hydrolysis of Plantago major, Plantago lanceolata, and Plantago media leaves with hemicellulase and xylanase was carried out.
32624876	6	80	theme	cupric	769:774	arg1	capacity					797:804	cupric reducing antioxidant capacity	769:804	cupric reducing antioxidant capacity	769:804	Their antioxidant activity was evaluated using 2,2-diphenyl-2-picryl-hydrazyl-hydrate, ferric reducing antioxidant power, and cupric reducing antioxidant capacity assays.
32624876	7	81	theme	plantarum	963:971	arg1	strains					983:989	Lactobacillus plantarum probiotic strains	949:989	Lactobacillus plantarum probiotic strains	949:989	The utilization of the carbohydrate complex from the obtained hydrolysates to stimulate the production of certain glycohydrolases from Lactobacillus plantarum probiotic strains was investigated.
32624876	10	82	theme	antioxidant	1255:1265	arg1	activity					1267:1274	The antioxidant activity	1251:1274	The antioxidant activity according to the three methods	1251:1305	The antioxidant activity according to the three methods was the highest in P. media hydrolysates, which correlated with their total phenolic concentration.
32624876	10	82	theme	antioxidant	1255:1265	arg1	highest					1315:1321	highest	1315:1321	highest	1315:1321	The antioxidant activity according to the three methods was the highest in P. media hydrolysates, which correlated with their total phenolic concentration.
31428752	1	0	theme	assisted	139:146	arg1	LDI-LIFT-TOF/TOF					148:163	Ti3C2 MXene assisted LDI-LIFT-TOF/TOF	127:163	Ti3C2 MXene assisted LDI-LIFT-TOF/TOF for robust differentiation and relative quantitation of glycan isomers that differ in composition, connectivity and configuration	127:293	Here we report Ti3C2 MXene assisted LDI-LIFT-TOF/TOF for robust differentiation and relative quantitation of glycan isomers that differ in composition, connectivity and configuration.
31428752	0	1	theme	isomers	91:97	arg1	quantitation					68:79	quantitation	68:79	quantitation	68:79	Ti3C2 MXene as a novel substrate provides rapid differentiation and quantitation of glycan isomers with LDI-MS.
31428752	0	1	theme	isomers	91:97	arg1	differentiation					48:62	rapid differentiation	42:62	rapid differentiation	42:62	Ti3C2 MXene as a novel substrate provides rapid differentiation and quantitation of glycan isomers with LDI-MS.
31428752	2	2	theme	improved	300:307	arg1	sensitivity					309:319	improved sensitivity	300:319	improved sensitivity	300:319	The improved sensitivity and background-free properties of this substrate combined with the special fragment profile produced by LDI-LIFT-TOF/TOF open a new door for nanomaterial assisted glycan structual analysis.
31428752	0	3	theme	glycan	84:89	arg1	isomers					91:97	glycan isomers	84:97	glycan isomers with LDI-MS	84:109	Ti3C2 MXene as a novel substrate provides rapid differentiation and quantitation of glycan isomers with LDI-MS.
31428752	2	4	theme	new	449:451	arg1	door					453:456	a new door	447:456	a new door for nanomaterial assisted glycan structual analysis	447:508	The improved sensitivity and background-free properties of this substrate combined with the special fragment profile produced by LDI-LIFT-TOF/TOF open a new door for nanomaterial assisted glycan structual analysis.
31428752	0	5	theme	Ti3C2	0:4	arg1	MXene					6:10	Ti3C2 MXene	0:10	Ti3C2 MXene as a novel substrate	0:31	Ti3C2 MXene as a novel substrate provides rapid differentiation and quantitation of glycan isomers with LDI-MS.
31428752	1	6	theme	robust	169:174	arg1	differentiation					176:190	robust differentiation	169:190	robust differentiation	169:190	Here we report Ti3C2 MXene assisted LDI-LIFT-TOF/TOF for robust differentiation and relative quantitation of glycan isomers that differ in composition, connectivity and configuration.
31428752	2	7	theme	glycan	484:489	arg1	analysis					501:508	nanomaterial assisted glycan structual analysis	462:508	nanomaterial assisted glycan structual analysis	462:508	The improved sensitivity and background-free properties of this substrate combined with the special fragment profile produced by LDI-LIFT-TOF/TOF open a new door for nanomaterial assisted glycan structual analysis.
31428752	2	8	theme	assisted	475:482	arg1	analysis					501:508	nanomaterial assisted glycan structual analysis	462:508	nanomaterial assisted glycan structual analysis	462:508	The improved sensitivity and background-free properties of this substrate combined with the special fragment profile produced by LDI-LIFT-TOF/TOF open a new door for nanomaterial assisted glycan structual analysis.
31428752	0	9	with	isomers	91:97	arg1	LDI-MS					104:109	LDI-MS	104:109	LDI-MS	104:109	Ti3C2 MXene as a novel substrate provides rapid differentiation and quantitation of glycan isomers with LDI-MS.
31428752	2	10	theme	background-free	325:339	arg1	properties					341:350	background-free properties	325:350	background-free properties	325:350	The improved sensitivity and background-free properties of this substrate combined with the special fragment profile produced by LDI-LIFT-TOF/TOF open a new door for nanomaterial assisted glycan structual analysis.
31428752	2	11	theme	nanomaterial	462:473	arg1	analysis					501:508	nanomaterial assisted glycan structual analysis	462:508	nanomaterial assisted glycan structual analysis	462:508	The improved sensitivity and background-free properties of this substrate combined with the special fragment profile produced by LDI-LIFT-TOF/TOF open a new door for nanomaterial assisted glycan structual analysis.
31428752	1	12	theme	relative	196:203	arg1	quantitation					205:216	relative quantitation	196:216	relative quantitation	196:216	Here we report Ti3C2 MXene assisted LDI-LIFT-TOF/TOF for robust differentiation and relative quantitation of glycan isomers that differ in composition, connectivity and configuration.
31428752	1	13	theme	Ti3C2	127:131	arg1	LDI-LIFT-TOF/TOF					148:163	Ti3C2 MXene assisted LDI-LIFT-TOF/TOF	127:163	Ti3C2 MXene assisted LDI-LIFT-TOF/TOF for robust differentiation and relative quantitation of glycan isomers that differ in composition, connectivity and configuration	127:293	Here we report Ti3C2 MXene assisted LDI-LIFT-TOF/TOF for robust differentiation and relative quantitation of glycan isomers that differ in composition, connectivity and configuration.
31428752	2	14	dep	sensitivity	309:319	arg1	The					296:298	The	296:298	The	296:298	The improved sensitivity and background-free properties of this substrate combined with the special fragment profile produced by LDI-LIFT-TOF/TOF open a new door for nanomaterial assisted glycan structual analysis.
31428752	0	15	theme	novel	17:21	arg1	substrate					23:31	a novel substrate	15:31	a novel substrate	15:31	Ti3C2 MXene as a novel substrate provides rapid differentiation and quantitation of glycan isomers with LDI-MS.
31428752	1	16	theme	glycan	221:226	arg1	isomers					228:234	glycan isomers	221:234	glycan isomers that differ in composition, connectivity and configuration	221:293	Here we report Ti3C2 MXene assisted LDI-LIFT-TOF/TOF for robust differentiation and relative quantitation of glycan isomers that differ in composition, connectivity and configuration.
31428752	0	17	theme	rapid	42:46	arg1	differentiation					48:62	rapid differentiation	42:62	rapid differentiation	42:62	Ti3C2 MXene as a novel substrate provides rapid differentiation and quantitation of glycan isomers with LDI-MS.
31428752	2	18	theme	structual	491:499	arg1	analysis					501:508	nanomaterial assisted glycan structual analysis	462:508	nanomaterial assisted glycan structual analysis	462:508	The improved sensitivity and background-free properties of this substrate combined with the special fragment profile produced by LDI-LIFT-TOF/TOF open a new door for nanomaterial assisted glycan structual analysis.
31428752	2	19	theme	substrate	360:368	arg1	sensitivity					309:319	improved sensitivity	300:319	improved sensitivity	300:319	The improved sensitivity and background-free properties of this substrate combined with the special fragment profile produced by LDI-LIFT-TOF/TOF open a new door for nanomaterial assisted glycan structual analysis.
31428752	2	19	theme	substrate	360:368	arg1	properties					341:350	background-free properties	325:350	background-free properties	325:350	The improved sensitivity and background-free properties of this substrate combined with the special fragment profile produced by LDI-LIFT-TOF/TOF open a new door for nanomaterial assisted glycan structual analysis.
31428752	1	20	theme	isomers	228:234	arg1	differentiation					176:190	robust differentiation	169:190	robust differentiation	169:190	Here we report Ti3C2 MXene assisted LDI-LIFT-TOF/TOF for robust differentiation and relative quantitation of glycan isomers that differ in composition, connectivity and configuration.
31428752	1	20	theme	isomers	228:234	arg1	quantitation					205:216	relative quantitation	196:216	relative quantitation	196:216	Here we report Ti3C2 MXene assisted LDI-LIFT-TOF/TOF for robust differentiation and relative quantitation of glycan isomers that differ in composition, connectivity and configuration.
31428752	1	21	theme	MXene	133:137	arg1	LDI-LIFT-TOF/TOF					148:163	Ti3C2 MXene assisted LDI-LIFT-TOF/TOF	127:163	Ti3C2 MXene assisted LDI-LIFT-TOF/TOF for robust differentiation and relative quantitation of glycan isomers that differ in composition, connectivity and configuration	127:293	Here we report Ti3C2 MXene assisted LDI-LIFT-TOF/TOF for robust differentiation and relative quantitation of glycan isomers that differ in composition, connectivity and configuration.
31428752	2	22	theme	fragment	396:403	arg1	profile					405:411	the special fragment profile	384:411	the special fragment profile produced by LDI-LIFT-TOF/TOF	384:440	The improved sensitivity and background-free properties of this substrate combined with the special fragment profile produced by LDI-LIFT-TOF/TOF open a new door for nanomaterial assisted glycan structual analysis.
31428752	2	23	theme	special	388:394	arg1	profile					405:411	the special fragment profile	384:411	the special fragment profile produced by LDI-LIFT-TOF/TOF	384:440	The improved sensitivity and background-free properties of this substrate combined with the special fragment profile produced by LDI-LIFT-TOF/TOF open a new door for nanomaterial assisted glycan structual analysis.
31726141	0	0	theme	bone	101:104	arg1	regeneration					106:117	bone regeneration	101:117	bone regeneration	101:117	Ginseng compound K incorporated porous Chitosan/biphasic calcium phosphate composite microsphere for bone regeneration.
31726141	7	1	used	used	1160:1163	arg2	alternative					1171:1181	an alternative	1168:1181	an alternative for the bone regeneration	1168:1207	The prepared biocomposite porous microsphere scaffold developed in this study can be used as an alternative for the bone regeneration or bone tissue engineering.
31726141	7	1	used	used	1160:1163	arg2	scaffold					1120:1127	The prepared biocomposite porous microsphere scaffold	1075:1127	The prepared biocomposite porous microsphere scaffold developed in this study	1075:1151	The prepared biocomposite porous microsphere scaffold developed in this study can be used as an alternative for the bone regeneration or bone tissue engineering.
31726141	7	1	used	used	1160:1163	arg2	engineering					1224:1234	bone tissue engineering	1212:1234	bone tissue engineering	1212:1234	The prepared biocomposite porous microsphere scaffold developed in this study can be used as an alternative for the bone regeneration or bone tissue engineering.
31726141	3	2	theme	apatite	523:529	arg1	crystal					531:537	the apatite crystal	519:537	the apatite crystal in the composite microsphere scaffolds	519:576	They were characterized using FTIR and XRD that showed the apatite crystal in the composite microsphere scaffolds were structurally similar to that of biogenic apatite crystals.
31726141	6	3	theme	microsphere	1032:1042	arg1	scaffolds					1044:1052	microsphere scaffolds	1032:1052	microsphere scaffolds compared to control	1032:1072	Further, Osteogenic markers expression was found to be higher in rat bone marrow stem cells seeded on microsphere scaffolds compared to control.
31726141	1	4	from	materials	162:170	arg1	field					188:192	the clinical field	175:192	the clinical field	175:192	There is a substantial for the bone graft materials in the clinical field.
31726141	2	5	theme	osteoconductivity	360:376	arg1	properties					378:387	mechanical and osteoconductivity properties	345:387	mechanical and osteoconductivity properties	345:387	Porous, stable and biodegradable bone microsphere scaffold using biopolymer chitosan was studied, and biphasic calcium phosphate was added to improve mechanical and osteoconductivity properties later ginseng compound K was added for improving its medicinal properties.
31726141	6	6	theme	markers	950:956	arg1	expression					958:967	Osteogenic markers expression	939:967	Osteogenic markers expression	939:967	Further, Osteogenic markers expression was found to be higher in rat bone marrow stem cells seeded on microsphere scaffolds compared to control.
31726141	3	7	from	crystal	531:537	arg1	scaffolds					568:576	the composite microsphere scaffolds	542:576	the composite microsphere scaffolds	542:576	They were characterized using FTIR and XRD that showed the apatite crystal in the composite microsphere scaffolds were structurally similar to that of biogenic apatite crystals.
31726141	2	8	theme	biphasic	297:304	arg1	phosphate					314:322	biphasic calcium phosphate	297:322	biphasic calcium phosphate	297:322	Porous, stable and biodegradable bone microsphere scaffold using biopolymer chitosan was studied, and biphasic calcium phosphate was added to improve mechanical and osteoconductivity properties later ginseng compound K was added for improving its medicinal properties.
31726141	6	9	theme	Osteogenic	939:948	arg1	expression					958:967	Osteogenic markers expression	939:967	Osteogenic markers expression	939:967	Further, Osteogenic markers expression was found to be higher in rat bone marrow stem cells seeded on microsphere scaffolds compared to control.
31726141	4	10	theme	composite	741:749	arg1	microspheres					751:762	the composite microspheres	737:762	the composite microspheres	737:762	Scanning electron microscopy images confirmed the presence of hydroxyapatite on the surface of the composite microspheres.
31726141	5	11	theme	MG63	856:859	arg1	cells					861:865	MG63 cells	856:865	MG63 cells	856:865	In vitro results infers that the composite microspheres are biocompatible with NIH 3T3 and MG63 cells and capable of supporting growth and spreading of MG-63 cells.
31726141	3	12	theme	composite	546:554	arg1	scaffolds					568:576	the composite microsphere scaffolds	542:576	the composite microsphere scaffolds	542:576	They were characterized using FTIR and XRD that showed the apatite crystal in the composite microsphere scaffolds were structurally similar to that of biogenic apatite crystals.
31726141	4	13	theme	hydroxyapatite	704:717	arg1	presence					692:699	the presence	688:699	the presence of hydroxyapatite on the surface of the composite microspheres	688:762	Scanning electron microscopy images confirmed the presence of hydroxyapatite on the surface of the composite microspheres.
31726141	3	14	theme	microsphere	556:566	arg1	scaffolds					568:576	the composite microsphere scaffolds	542:576	the composite microsphere scaffolds	542:576	They were characterized using FTIR and XRD that showed the apatite crystal in the composite microsphere scaffolds were structurally similar to that of biogenic apatite crystals.
31726141	1	15	theme	clinical	179:186	arg1	field					188:192	the clinical field	175:192	the clinical field	175:192	There is a substantial for the bone graft materials in the clinical field.
31726141	0	16	theme	compound	8:15	arg1	K					17:17	Ginseng compound K	0:17	Ginseng compound K	0:17	Ginseng compound K incorporated porous Chitosan/biphasic calcium phosphate composite microsphere for bone regeneration.
31726141	6	17	theme	rat	995:997	arg1	cells					1016:1020	rat bone marrow stem cells	995:1020	rat bone marrow stem cells seeded on microsphere scaffolds compared to control	995:1072	Further, Osteogenic markers expression was found to be higher in rat bone marrow stem cells seeded on microsphere scaffolds compared to control.
31726141	5	18	dep	In	765:766	arg1	vitro					768:772	vitro	768:772	vitro	768:772	In vitro results infers that the composite microspheres are biocompatible with NIH 3T3 and MG63 cells and capable of supporting growth and spreading of MG-63 cells.
31726141	7	19	theme	bone	1212:1215	arg1	engineering					1224:1234	bone tissue engineering	1212:1234	bone tissue engineering	1212:1234	The prepared biocomposite porous microsphere scaffold developed in this study can be used as an alternative for the bone regeneration or bone tissue engineering.
31726141	7	19	theme	bone	1212:1215	arg1	scaffold					1120:1127	The prepared biocomposite porous microsphere scaffold	1075:1127	The prepared biocomposite porous microsphere scaffold developed in this study	1075:1151	The prepared biocomposite porous microsphere scaffold developed in this study can be used as an alternative for the bone regeneration or bone tissue engineering.
31726141	5	20	theme	MG-63	917:921	arg1	cells					923:927	MG-63 cells	917:927	MG-63 cells	917:927	In vitro results infers that the composite microspheres are biocompatible with NIH 3T3 and MG63 cells and capable of supporting growth and spreading of MG-63 cells.
31726141	0	21	theme	Ginseng	0:6	arg1	K					17:17	Ginseng compound K	0:17	Ginseng compound K	0:17	Ginseng compound K incorporated porous Chitosan/biphasic calcium phosphate composite microsphere for bone regeneration.
31726141	6	22	theme	stem	1011:1014	arg1	cells					1016:1020	rat bone marrow stem cells	995:1020	rat bone marrow stem cells seeded on microsphere scaffolds compared to control	995:1072	Further, Osteogenic markers expression was found to be higher in rat bone marrow stem cells seeded on microsphere scaffolds compared to control.
31726141	4	23	attach	presence	692:699	arg2	hydroxyapatite					704:717	hydroxyapatite	704:717	hydroxyapatite	704:717	Scanning electron microscopy images confirmed the presence of hydroxyapatite on the surface of the composite microspheres.
31726141	4	23	attach	presence	692:699	arg1	surface					726:732	the surface	722:732	the surface of the composite microspheres	722:762	Scanning electron microscopy images confirmed the presence of hydroxyapatite on the surface of the composite microspheres.
31726141	4	24	from	presence	692:699	arg1	surface					726:732	the surface	722:732	the surface of the composite microspheres	722:762	Scanning electron microscopy images confirmed the presence of hydroxyapatite on the surface of the composite microspheres.
31726141	2	25	theme	compound	403:410	arg1	K					412:412	later ginseng compound K	389:412	later ginseng compound K	389:412	Porous, stable and biodegradable bone microsphere scaffold using biopolymer chitosan was studied, and biphasic calcium phosphate was added to improve mechanical and osteoconductivity properties later ginseng compound K was added for improving its medicinal properties.
31726141	6	26	theme	marrow	1004:1009	arg1	cells					1016:1020	rat bone marrow stem cells	995:1020	rat bone marrow stem cells seeded on microsphere scaffolds compared to control	995:1072	Further, Osteogenic markers expression was found to be higher in rat bone marrow stem cells seeded on microsphere scaffolds compared to control.
31726141	3	27	theme	biogenic	615:622	arg1	crystals					632:639	biogenic apatite crystals	615:639	biogenic apatite crystals	615:639	They were characterized using FTIR and XRD that showed the apatite crystal in the composite microsphere scaffolds were structurally similar to that of biogenic apatite crystals.
31726141	2	28	theme	medicinal	442:450	arg1	properties					452:461	its medicinal properties	438:461	its medicinal properties	438:461	Porous, stable and biodegradable bone microsphere scaffold using biopolymer chitosan was studied, and biphasic calcium phosphate was added to improve mechanical and osteoconductivity properties later ginseng compound K was added for improving its medicinal properties.
31726141	6	29	theme	bone	999:1002	arg1	cells					1016:1020	rat bone marrow stem cells	995:1020	rat bone marrow stem cells seeded on microsphere scaffolds compared to control	995:1072	Further, Osteogenic markers expression was found to be higher in rat bone marrow stem cells seeded on microsphere scaffolds compared to control.
31726141	3	30	dep	showed	512:517	arg1	similar					596:602	similar	596:602	similar	596:602	They were characterized using FTIR and XRD that showed the apatite crystal in the composite microsphere scaffolds were structurally similar to that of biogenic apatite crystals.
31726141	2	31	theme	calcium	306:312	arg1	phosphate					314:322	biphasic calcium phosphate	297:322	biphasic calcium phosphate	297:322	Porous, stable and biodegradable bone microsphere scaffold using biopolymer chitosan was studied, and biphasic calcium phosphate was added to improve mechanical and osteoconductivity properties later ginseng compound K was added for improving its medicinal properties.
31726141	7	32	theme	bone	1191:1194	arg1	regeneration					1196:1207	the bone regeneration	1187:1207	the bone regeneration	1187:1207	The prepared biocomposite porous microsphere scaffold developed in this study can be used as an alternative for the bone regeneration or bone tissue engineering.
31726141	1	33	theme	bone	151:154	arg1	materials					162:170	the bone graft materials	147:170	the bone graft materials in the clinical field	147:192	There is a substantial for the bone graft materials in the clinical field.
31726141	0	34	theme	Chitosan/biphasic	39:55	arg1	microsphere					85:95	porous Chitosan/biphasic calcium phosphate composite microsphere	32:95	porous Chitosan/biphasic calcium phosphate composite microsphere for bone regeneration	32:117	Ginseng compound K incorporated porous Chitosan/biphasic calcium phosphate composite microsphere for bone regeneration.
31726141	4	35	theme	microspheres	751:762	arg1	surface					726:732	the surface	722:732	the surface of the composite microspheres	722:762	Scanning electron microscopy images confirmed the presence of hydroxyapatite on the surface of the composite microspheres.
31726141	5	36	theme	In	765:766	arg1	results					774:780	In vitro results	765:780	In vitro results	765:780	In vitro results infers that the composite microspheres are biocompatible with NIH 3T3 and MG63 cells and capable of supporting growth and spreading of MG-63 cells.
31726141	7	37	theme	tissue	1217:1222	arg1	engineering					1224:1234	bone tissue engineering	1212:1234	bone tissue engineering	1212:1234	The prepared biocomposite porous microsphere scaffold developed in this study can be used as an alternative for the bone regeneration or bone tissue engineering.
31726141	7	37	theme	tissue	1217:1222	arg1	scaffold					1120:1127	The prepared biocomposite porous microsphere scaffold	1075:1127	The prepared biocomposite porous microsphere scaffold developed in this study	1075:1151	The prepared biocomposite porous microsphere scaffold developed in this study can be used as an alternative for the bone regeneration or bone tissue engineering.
31726141	3	38	theme	apatite	624:630	arg1	crystals					632:639	biogenic apatite crystals	615:639	biogenic apatite crystals	615:639	They were characterized using FTIR and XRD that showed the apatite crystal in the composite microsphere scaffolds were structurally similar to that of biogenic apatite crystals.
31726141	5	39	theme	cells	923:927	arg1	growth					893:898	growth	893:898	growth	893:898	In vitro results infers that the composite microspheres are biocompatible with NIH 3T3 and MG63 cells and capable of supporting growth and spreading of MG-63 cells.
31726141	5	39	theme	cells	923:927	arg1	spreading					904:912	spreading	904:912	spreading	904:912	In vitro results infers that the composite microspheres are biocompatible with NIH 3T3 and MG63 cells and capable of supporting growth and spreading of MG-63 cells.
31726141	0	40	theme	porous	32:37	arg1	microsphere					85:95	porous Chitosan/biphasic calcium phosphate composite microsphere	32:95	porous Chitosan/biphasic calcium phosphate composite microsphere for bone regeneration	32:117	Ginseng compound K incorporated porous Chitosan/biphasic calcium phosphate composite microsphere for bone regeneration.
31726141	5	41	theme	NIH	844:846	arg1	3T3					848:850	NIH 3T3	844:850	NIH 3T3	844:850	In vitro results infers that the composite microspheres are biocompatible with NIH 3T3 and MG63 cells and capable of supporting growth and spreading of MG-63 cells.
31726141	2	42	theme	microsphere	233:243	arg1	scaffold					245:252	Porous, stable and biodegradable bone microsphere scaffold	195:252	Porous, stable and biodegradable bone microsphere scaffold using biopolymer chitosan	195:278	Porous, stable and biodegradable bone microsphere scaffold using biopolymer chitosan was studied, and biphasic calcium phosphate was added to improve mechanical and osteoconductivity properties later ginseng compound K was added for improving its medicinal properties.
31726141	0	43	theme	phosphate	65:73	arg1	microsphere					85:95	porous Chitosan/biphasic calcium phosphate composite microsphere	32:95	porous Chitosan/biphasic calcium phosphate composite microsphere for bone regeneration	32:117	Ginseng compound K incorporated porous Chitosan/biphasic calcium phosphate composite microsphere for bone regeneration.
31726141	7	44	theme	prepared	1079:1086	arg1	alternative					1171:1181	an alternative	1168:1181	an alternative for the bone regeneration	1168:1207	The prepared biocomposite porous microsphere scaffold developed in this study can be used as an alternative for the bone regeneration or bone tissue engineering.
31726141	7	44	theme	prepared	1079:1086	arg1	scaffold					1120:1127	The prepared biocomposite porous microsphere scaffold	1075:1127	The prepared biocomposite porous microsphere scaffold developed in this study	1075:1151	The prepared biocomposite porous microsphere scaffold developed in this study can be used as an alternative for the bone regeneration or bone tissue engineering.
31726141	7	44	theme	prepared	1079:1086	arg1	engineering					1224:1234	bone tissue engineering	1212:1234	bone tissue engineering	1212:1234	The prepared biocomposite porous microsphere scaffold developed in this study can be used as an alternative for the bone regeneration or bone tissue engineering.
31726141	2	45	theme	bone	228:231	arg1	scaffold					245:252	Porous, stable and biodegradable bone microsphere scaffold	195:252	Porous, stable and biodegradable bone microsphere scaffold using biopolymer chitosan	195:278	Porous, stable and biodegradable bone microsphere scaffold using biopolymer chitosan was studied, and biphasic calcium phosphate was added to improve mechanical and osteoconductivity properties later ginseng compound K was added for improving its medicinal properties.
31726141	7	46	theme	microsphere	1108:1118	arg1	alternative					1171:1181	an alternative	1168:1181	an alternative for the bone regeneration	1168:1207	The prepared biocomposite porous microsphere scaffold developed in this study can be used as an alternative for the bone regeneration or bone tissue engineering.
31726141	7	46	theme	microsphere	1108:1118	arg1	scaffold					1120:1127	The prepared biocomposite porous microsphere scaffold	1075:1127	The prepared biocomposite porous microsphere scaffold developed in this study	1075:1151	The prepared biocomposite porous microsphere scaffold developed in this study can be used as an alternative for the bone regeneration or bone tissue engineering.
31726141	7	46	theme	microsphere	1108:1118	arg1	engineering					1224:1234	bone tissue engineering	1212:1234	bone tissue engineering	1212:1234	The prepared biocomposite porous microsphere scaffold developed in this study can be used as an alternative for the bone regeneration or bone tissue engineering.
31726141	0	47	theme	calcium	57:63	arg1	microsphere					85:95	porous Chitosan/biphasic calcium phosphate composite microsphere	32:95	porous Chitosan/biphasic calcium phosphate composite microsphere for bone regeneration	32:117	Ginseng compound K incorporated porous Chitosan/biphasic calcium phosphate composite microsphere for bone regeneration.
31726141	7	48	theme	biocomposite	1088:1099	arg1	alternative					1171:1181	an alternative	1168:1181	an alternative for the bone regeneration	1168:1207	The prepared biocomposite porous microsphere scaffold developed in this study can be used as an alternative for the bone regeneration or bone tissue engineering.
31726141	7	48	theme	biocomposite	1088:1099	arg1	scaffold					1120:1127	The prepared biocomposite porous microsphere scaffold	1075:1127	The prepared biocomposite porous microsphere scaffold developed in this study	1075:1151	The prepared biocomposite porous microsphere scaffold developed in this study can be used as an alternative for the bone regeneration or bone tissue engineering.
31726141	7	48	theme	biocomposite	1088:1099	arg1	engineering					1224:1234	bone tissue engineering	1212:1234	bone tissue engineering	1212:1234	The prepared biocomposite porous microsphere scaffold developed in this study can be used as an alternative for the bone regeneration or bone tissue engineering.
31726141	4	49	dep	microscopy	660:669	arg1	images					671:676	images	671:676	images	671:676	Scanning electron microscopy images confirmed the presence of hydroxyapatite on the surface of the composite microspheres.
31726141	2	50	theme	biodegradable	214:226	arg1	scaffold					245:252	Porous, stable and biodegradable bone microsphere scaffold	195:252	Porous, stable and biodegradable bone microsphere scaffold using biopolymer chitosan	195:278	Porous, stable and biodegradable bone microsphere scaffold using biopolymer chitosan was studied, and biphasic calcium phosphate was added to improve mechanical and osteoconductivity properties later ginseng compound K was added for improving its medicinal properties.
31726141	2	51	theme	mechanical	345:354	arg1	properties					378:387	mechanical and osteoconductivity properties	345:387	mechanical and osteoconductivity properties	345:387	Porous, stable and biodegradable bone microsphere scaffold using biopolymer chitosan was studied, and biphasic calcium phosphate was added to improve mechanical and osteoconductivity properties later ginseng compound K was added for improving its medicinal properties.
31726141	2	52	theme	stable	203:208	arg1	scaffold					245:252	Porous, stable and biodegradable bone microsphere scaffold	195:252	Porous, stable and biodegradable bone microsphere scaffold using biopolymer chitosan	195:278	Porous, stable and biodegradable bone microsphere scaffold using biopolymer chitosan was studied, and biphasic calcium phosphate was added to improve mechanical and osteoconductivity properties later ginseng compound K was added for improving its medicinal properties.
31726141	4	53	theme	Scanning	642:649	arg1	microscopy					660:669	Scanning electron microscopy	642:669	Scanning electron microscopy images	642:676	Scanning electron microscopy images confirmed the presence of hydroxyapatite on the surface of the composite microspheres.
31726141	5	54	with	biocompatible	825:837	arg1	3T3					848:850	NIH 3T3	844:850	NIH 3T3	844:850	In vitro results infers that the composite microspheres are biocompatible with NIH 3T3 and MG63 cells and capable of supporting growth and spreading of MG-63 cells.
31726141	5	54	with	biocompatible	825:837	arg1	cells					861:865	MG63 cells	856:865	MG63 cells	856:865	In vitro results infers that the composite microspheres are biocompatible with NIH 3T3 and MG63 cells and capable of supporting growth and spreading of MG-63 cells.
31726141	1	55	theme	graft	156:160	arg1	materials					162:170	the bone graft materials	147:170	the bone graft materials in the clinical field	147:192	There is a substantial for the bone graft materials in the clinical field.
31726141	5	56	theme	composite	798:806	arg1	microspheres					808:819	the composite microspheres	794:819	the composite microspheres	794:819	In vitro results infers that the composite microspheres are biocompatible with NIH 3T3 and MG63 cells and capable of supporting growth and spreading of MG-63 cells.
31726141	5	56	theme	composite	798:806	arg1	biocompatible					825:837	biocompatible	825:837	biocompatible	825:837	In vitro results infers that the composite microspheres are biocompatible with NIH 3T3 and MG63 cells and capable of supporting growth and spreading of MG-63 cells.
31726141	2	57	theme	Porous	195:200	arg1	scaffold					245:252	Porous, stable and biodegradable bone microsphere scaffold	195:252	Porous, stable and biodegradable bone microsphere scaffold using biopolymer chitosan	195:278	Porous, stable and biodegradable bone microsphere scaffold using biopolymer chitosan was studied, and biphasic calcium phosphate was added to improve mechanical and osteoconductivity properties later ginseng compound K was added for improving its medicinal properties.
31726141	2	58	theme	biopolymer	260:269	arg1	chitosan					271:278	biopolymer chitosan	260:278	biopolymer chitosan	260:278	Porous, stable and biodegradable bone microsphere scaffold using biopolymer chitosan was studied, and biphasic calcium phosphate was added to improve mechanical and osteoconductivity properties later ginseng compound K was added for improving its medicinal properties.
31726141	2	59	theme	ginseng	395:401	arg1	K					412:412	later ginseng compound K	389:412	later ginseng compound K	389:412	Porous, stable and biodegradable bone microsphere scaffold using biopolymer chitosan was studied, and biphasic calcium phosphate was added to improve mechanical and osteoconductivity properties later ginseng compound K was added for improving its medicinal properties.
31726141	0	60	theme	composite	75:83	arg1	microsphere					85:95	porous Chitosan/biphasic calcium phosphate composite microsphere	32:95	porous Chitosan/biphasic calcium phosphate composite microsphere for bone regeneration	32:117	Ginseng compound K incorporated porous Chitosan/biphasic calcium phosphate composite microsphere for bone regeneration.
31726141	7	61	theme	porous	1101:1106	arg1	alternative					1171:1181	an alternative	1168:1181	an alternative for the bone regeneration	1168:1207	The prepared biocomposite porous microsphere scaffold developed in this study can be used as an alternative for the bone regeneration or bone tissue engineering.
31726141	7	61	theme	porous	1101:1106	arg1	scaffold					1120:1127	The prepared biocomposite porous microsphere scaffold	1075:1127	The prepared biocomposite porous microsphere scaffold developed in this study	1075:1151	The prepared biocomposite porous microsphere scaffold developed in this study can be used as an alternative for the bone regeneration or bone tissue engineering.
31726141	7	61	theme	porous	1101:1106	arg1	engineering					1224:1234	bone tissue engineering	1212:1234	bone tissue engineering	1212:1234	The prepared biocomposite porous microsphere scaffold developed in this study can be used as an alternative for the bone regeneration or bone tissue engineering.
31726141	2	62	theme	later	389:393	arg1	K					412:412	later ginseng compound K	389:412	later ginseng compound K	389:412	Porous, stable and biodegradable bone microsphere scaffold using biopolymer chitosan was studied, and biphasic calcium phosphate was added to improve mechanical and osteoconductivity properties later ginseng compound K was added for improving its medicinal properties.
31726141	4	63	theme	electron	651:658	arg1	microscopy					660:669	Scanning electron microscopy	642:669	Scanning electron microscopy images	642:676	Scanning electron microscopy images confirmed the presence of hydroxyapatite on the surface of the composite microspheres.
29378055	11	0	theme	body	2051:2054	arg1	proteins					2056:2063	sparing body proteins	2043:2063	sparing body proteins	2043:2063	This could be advantageous in sparing body proteins, particularly during catabolic states, such as those related to malnutrition during aging.
29378055	2	1	theme	acids	360:364	arg1	use					347:349	the use	343:349	the use of amino acids (AAs) as energy substrates	343:391	However, its consumption in the postabsorptive state might contribute to reducing the use of amino acids (AAs) as energy substrates and thus spare nitrogen resources, which could be beneficial during catabolic states.
29378055	8	2	theme	branched-chain	1469:1482	arg1	concentrations					1487:1500	circulating branched-chain AA concentrations	1457:1500	circulating branched-chain AA concentrations	1457:1500	Fructose feeding also reduced circulating branched-chain AA concentrations by 50% (compared with PER) and increased those of alanine (+65% compared with PER).
29378055	3	3	from	feeding	519:525	arg1	rats					615:618	older rats	609:618	older rats	609:618	Objective We hypothesized that fructose feeding during a catabolic situation corresponding to protein-energy restriction (PER) in older rats would reduce AA utilization for energy purposes, thus slowing down the loss of body weight (BW) and improving body composition.
29378055	2	4	theme	postabsorptive	293:306	arg1	state					308:312	the postabsorptive state	289:312	the postabsorptive state	289:312	However, its consumption in the postabsorptive state might contribute to reducing the use of amino acids (AAs) as energy substrates and thus spare nitrogen resources, which could be beneficial during catabolic states.
29378055	3	5	theme	weight	704:709	arg1	loss					691:694	the loss	687:694	the loss of body weight (BW)	687:714	Objective We hypothesized that fructose feeding during a catabolic situation corresponding to protein-energy restriction (PER) in older rats would reduce AA utilization for energy purposes, thus slowing down the loss of body weight (BW) and improving body composition.
29378055	3	6	theme	catabolic	536:544	arg1	situation					546:554	a catabolic situation	534:554	a catabolic situation corresponding to protein-energy restriction (PER)	534:604	Objective We hypothesized that fructose feeding during a catabolic situation corresponding to protein-energy restriction (PER) in older rats would reduce AA utilization for energy purposes, thus slowing down the loss of body weight (BW) and improving body composition.
29378055	6	7	theme	mass	1317:1320	arg1	loss					1305:1308	the loss	1301:1308	the loss of fat mass (-28% compared with PER)	1301:1345	Results Both glucose and fructose feeding reduced PER-induced loss of BW and lean mass (-27% compared with PER), but only fructose reduced the loss of fat mass (-28% compared with PER).
29378055	1	8	theme	energy	183:188	arg1	intake					190:195	high energy intake	178:195	high energy intake	178:195	Background Fructose feeding in the context of high energy intake is recognized as being responsible for metabolic dysregulation.
29378055	10	9	theme	liver	1986:1990	arg1	potential					2002:2010	liver autophagy potential	1986:2010	liver autophagy potential	1986:2010	Conclusions We showed that in PER older rats, fructose feeding improved body composition and the weight of several organs by reducing AA catabolism and utilization for energy production and liver autophagy potential.
29378055	3	10	theme	body	730:733	arg1	composition					735:745	body composition	730:745	body composition	730:745	Objective We hypothesized that fructose feeding during a catabolic situation corresponding to protein-energy restriction (PER) in older rats would reduce AA utilization for energy purposes, thus slowing down the loss of body weight (BW) and improving body composition.
29378055	7	11	theme	intestinal	1410:1419	arg1	mass					1421:1424	intestinal mass	1410:1424	intestinal mass	1410:1424	Fructose feeding prevented the PER-induced loss of muscle and intestinal mass.
29378055	9	12	from	reduction	1588:1596	arg1	enzymes					1609:1615	hepatic enzymes	1601:1615	hepatic enzymes related to AA catabolism	1601:1640	A reduction in hepatic enzymes related to AA catabolism was also observed during fructose feeding (compared with PER), whereas glycogen concentrations were enhanced in both intestine (+300%) and muscle (+21%).
29378055	3	13	theme	protein-energy	573:586	arg1	restriction					588:598	protein-energy restriction	573:598	protein-energy restriction (PER)	573:604	Objective We hypothesized that fructose feeding during a catabolic situation corresponding to protein-energy restriction (PER) in older rats would reduce AA utilization for energy purposes, thus slowing down the loss of body weight (BW) and improving body composition.
29378055	7	14	theme	muscle	1399:1404	arg1	loss					1391:1394	the PER-induced loss	1375:1394	the PER-induced loss of muscle and intestinal mass	1375:1424	Fructose feeding prevented the PER-induced loss of muscle and intestinal mass.
29378055	10	15	theme	PER	1826:1828	arg1	rats					1836:1839	PER older rats	1826:1839	PER older rats	1826:1839	Conclusions We showed that in PER older rats, fructose feeding improved body composition and the weight of several organs by reducing AA catabolism and utilization for energy production and liver autophagy potential.
29378055	3	16	theme	older	609:613	arg1	rats					615:618	older rats	609:618	older rats	609:618	Objective We hypothesized that fructose feeding during a catabolic situation corresponding to protein-energy restriction (PER) in older rats would reduce AA utilization for energy purposes, thus slowing down the loss of body weight (BW) and improving body composition.
29378055	6	17	dep	Results	1162:1168	arg1	glucose					1175:1181	glucose	1175:1181	glucose	1175:1181	Results Both glucose and fructose feeding reduced PER-induced loss of BW and lean mass (-27% compared with PER), but only fructose reduced the loss of fat mass (-28% compared with PER).
29378055	6	17	dep	Results	1162:1168	arg1	feeding					1196:1202	fructose feeding	1187:1202	fructose feeding	1187:1202	Results Both glucose and fructose feeding reduced PER-induced loss of BW and lean mass (-27% compared with PER), but only fructose reduced the loss of fat mass (-28% compared with PER).
29378055	6	17	dep	Results	1162:1168	arg1	Results					1162:1168	Results Both glucose and fructose feeding	1162:1202	Results Both glucose and fructose feeding	1162:1202	Results Both glucose and fructose feeding reduced PER-induced loss of BW and lean mass (-27% compared with PER), but only fructose reduced the loss of fat mass (-28% compared with PER).
29378055	1	18	theme	Background	132:141	arg1	feeding					152:158	Background Fructose feeding	132:158	Background Fructose feeding in the context of high energy intake	132:195	Background Fructose feeding in the context of high energy intake is recognized as being responsible for metabolic dysregulation.
29378055	4	19	theme	13	844:845	arg1	%					846:846	%	846:846	%	846:846	Methods For 45 d, 22-mo-old male Wistar rats (average weight: 716 g) were fed a control ration (13% protein) either at normal (20 g/d), restricted (PER: 10 g/d), or at PER levels supplemented with glucose (3 g/d) or fructose (3 g/d) and then studied in the postabsorptive state.
29378055	10	20	theme	fructose	1842:1849	arg1	feeding					1851:1857	fructose feeding	1842:1857	fructose feeding	1842:1857	Conclusions We showed that in PER older rats, fructose feeding improved body composition and the weight of several organs by reducing AA catabolism and utilization for energy production and liver autophagy potential.
29378055	5	21	theme	related	1113:1119	arg1	activities					1072:1081	enzyme activities	1065:1081	enzyme activities	1065:1081	We measured BW, body composition, and enzyme activities and metabolite concentrations related to glucose, fructose, and AA metabolism.
29378055	3	22	theme	AA	633:634	arg1	utilization					636:646	AA utilization	633:646	AA utilization for energy purposes	633:666	Objective We hypothesized that fructose feeding during a catabolic situation corresponding to protein-energy restriction (PER) in older rats would reduce AA utilization for energy purposes, thus slowing down the loss of body weight (BW) and improving body composition.
29378055	9	23	theme	glycogen	1713:1720	arg1	concentrations					1722:1735	glycogen concentrations	1713:1735	glycogen concentrations	1713:1735	A reduction in hepatic enzymes related to AA catabolism was also observed during fructose feeding (compared with PER), whereas glycogen concentrations were enhanced in both intestine (+300%) and muscle (+21%).
29378055	6	24	theme	PER-induced	1212:1222	arg1	loss					1224:1227	PER-induced loss	1212:1227	PER-induced loss of BW	1212:1233	Results Both glucose and fructose feeding reduced PER-induced loss of BW and lean mass (-27% compared with PER), but only fructose reduced the loss of fat mass (-28% compared with PER).
29378055	10	25	theme	organs	1911:1916	arg1	composition					1873:1883	body composition	1868:1883	body composition	1868:1883	Conclusions We showed that in PER older rats, fructose feeding improved body composition and the weight of several organs by reducing AA catabolism and utilization for energy production and liver autophagy potential.
29378055	10	25	theme	organs	1911:1916	arg1	weight					1893:1898	the weight	1889:1898	the weight of several organs	1889:1916	Conclusions We showed that in PER older rats, fructose feeding improved body composition and the weight of several organs by reducing AA catabolism and utilization for energy production and liver autophagy potential.
29378055	5	26	theme	metabolite	1087:1096	arg1	concentrations					1098:1111	metabolite concentrations	1087:1111	metabolite concentrations	1087:1111	We measured BW, body composition, and enzyme activities and metabolite concentrations related to glucose, fructose, and AA metabolism.
29378055	3	27	dep	Objective	479:487	arg1	hypothesized					492:503	hypothesized	492:503	hypothesized that fructose feeding during a catabolic situation corresponding to protein-energy restriction (PER) in older rats would reduce AA utilization for energy purposes, thus slowing down the loss of body weight (BW) and improving body composition	492:745	Objective We hypothesized that fructose feeding during a catabolic situation corresponding to protein-energy restriction (PER) in older rats would reduce AA utilization for energy purposes, thus slowing down the loss of body weight (BW) and improving body composition.
29378055	0	28	theme	Old	122:124	arg1	Rats					126:129	Protein-Energy-Restricted Old Rats	96:129	Protein-Energy-Restricted Old Rats	96:129	Fructose Feeding during the Postabsorptive State Alters Body Composition and Spares Nitrogen in Protein-Energy-Restricted Old Rats.
29378055	2	29	theme	energy	375:380	arg1	substrates					382:391	energy substrates	375:391	energy substrates	375:391	However, its consumption in the postabsorptive state might contribute to reducing the use of amino acids (AAs) as energy substrates and thus spare nitrogen resources, which could be beneficial during catabolic states.
29378055	0	30	theme	Fructose	0:7	arg1	Feeding					9:15	Fructose Feeding	0:15	Fructose Feeding during the Postabsorptive State	0:47	Fructose Feeding during the Postabsorptive State Alters Body Composition and Spares Nitrogen in Protein-Energy-Restricted Old Rats.
29378055	4	31	dep	weight	802:807	arg1	g					814:814	716 g	810:814	average weight: 716 g	794:814	Methods For 45 d, 22-mo-old male Wistar rats (average weight: 716 g) were fed a control ration (13% protein) either at normal (20 g/d), restricted (PER: 10 g/d), or at PER levels supplemented with glucose (3 g/d) or fructose (3 g/d) and then studied in the postabsorptive state.
29378055	2	32	from	consumption	274:284	arg1	state					308:312	the postabsorptive state	289:312	the postabsorptive state	289:312	However, its consumption in the postabsorptive state might contribute to reducing the use of amino acids (AAs) as energy substrates and thus spare nitrogen resources, which could be beneficial during catabolic states.
29378055	2	33	theme	spare	402:406	arg1	resources					417:425	spare nitrogen resources	402:425	spare nitrogen resources	402:425	However, its consumption in the postabsorptive state might contribute to reducing the use of amino acids (AAs) as energy substrates and thus spare nitrogen resources, which could be beneficial during catabolic states.
29378055	0	34	theme	Postabsorptive	28:41	arg1	State					43:47	the Postabsorptive State	24:47	the Postabsorptive State	24:47	Fructose Feeding during the Postabsorptive State Alters Body Composition and Spares Nitrogen in Protein-Energy-Restricted Old Rats.
29378055	4	35	theme	average	794:800	arg1	weight					802:807	average weight	794:807	average weight: 716 g	794:814	Methods For 45 d, 22-mo-old male Wistar rats (average weight: 716 g) were fed a control ration (13% protein) either at normal (20 g/d), restricted (PER: 10 g/d), or at PER levels supplemented with glucose (3 g/d) or fructose (3 g/d) and then studied in the postabsorptive state.
29378055	2	36	theme	amino	354:358	arg1	AAs					367:369	AAs	367:369	AAs	367:369	However, its consumption in the postabsorptive state might contribute to reducing the use of amino acids (AAs) as energy substrates and thus spare nitrogen resources, which could be beneficial during catabolic states.
29378055	2	36	theme	amino	354:358	arg1	acids					360:364	amino acids	354:364	amino acids (AAs)	354:370	However, its consumption in the postabsorptive state might contribute to reducing the use of amino acids (AAs) as energy substrates and thus spare nitrogen resources, which could be beneficial during catabolic states.
29378055	6	37	dep	mass	1244:1247	arg1	%					1253:1253	-27%	1250:1253	-27% compared with PER	1250:1271	Results Both glucose and fructose feeding reduced PER-induced loss of BW and lean mass (-27% compared with PER), but only fructose reduced the loss of fat mass (-28% compared with PER).
29378055	5	38	theme	body	1043:1046	arg1	composition					1048:1058	body composition	1043:1058	body composition	1043:1058	We measured BW, body composition, and enzyme activities and metabolite concentrations related to glucose, fructose, and AA metabolism.
29378055	9	39	theme	hepatic	1601:1607	arg1	enzymes					1609:1615	hepatic enzymes	1601:1615	hepatic enzymes related to AA catabolism	1601:1640	A reduction in hepatic enzymes related to AA catabolism was also observed during fructose feeding (compared with PER), whereas glycogen concentrations were enhanced in both intestine (+300%) and muscle (+21%).
29378055	4	40	theme	male	776:779	arg1	rats					788:791	22-mo-old male Wistar rats	766:791	22-mo-old male Wistar rats (average weight: 716 g)	766:815	Methods For 45 d, 22-mo-old male Wistar rats (average weight: 716 g) were fed a control ration (13% protein) either at normal (20 g/d), restricted (PER: 10 g/d), or at PER levels supplemented with glucose (3 g/d) or fructose (3 g/d) and then studied in the postabsorptive state.
29378055	8	41	theme	Fructose	1427:1434	arg1	feeding					1436:1442	Fructose feeding	1427:1442	Fructose feeding	1427:1442	Fructose feeding also reduced circulating branched-chain AA concentrations by 50% (compared with PER) and increased those of alanine (+65% compared with PER).
29378055	6	42	theme	BW	1232:1233	arg1	mass					1244:1247	lean mass	1239:1247	lean mass (-27% compared with PER)	1239:1272	Results Both glucose and fructose feeding reduced PER-induced loss of BW and lean mass (-27% compared with PER), but only fructose reduced the loss of fat mass (-28% compared with PER).
29378055	6	42	theme	BW	1232:1233	arg1	loss					1224:1227	PER-induced loss	1212:1227	PER-induced loss of BW	1212:1233	Results Both glucose and fructose feeding reduced PER-induced loss of BW and lean mass (-27% compared with PER), but only fructose reduced the loss of fat mass (-28% compared with PER).
29378055	9	43	theme	related	1617:1623	arg1	enzymes					1609:1615	hepatic enzymes	1601:1615	hepatic enzymes related to AA catabolism	1601:1640	A reduction in hepatic enzymes related to AA catabolism was also observed during fructose feeding (compared with PER), whereas glycogen concentrations were enhanced in both intestine (+300%) and muscle (+21%).
29378055	3	44	theme	body	699:702	arg1	BW					712:713	BW	712:713	BW	712:713	Objective We hypothesized that fructose feeding during a catabolic situation corresponding to protein-energy restriction (PER) in older rats would reduce AA utilization for energy purposes, thus slowing down the loss of body weight (BW) and improving body composition.
29378055	3	44	theme	body	699:702	arg1	weight					704:709	body weight	699:709	body weight (BW)	699:714	Objective We hypothesized that fructose feeding during a catabolic situation corresponding to protein-energy restriction (PER) in older rats would reduce AA utilization for energy purposes, thus slowing down the loss of body weight (BW) and improving body composition.
29378055	10	45	theme	energy	1964:1969	arg1	production					1971:1980	energy production	1964:1980	energy production	1964:1980	Conclusions We showed that in PER older rats, fructose feeding improved body composition and the weight of several organs by reducing AA catabolism and utilization for energy production and liver autophagy potential.
29378055	9	46	theme	AA	1628:1629	arg1	catabolism					1631:1640	AA catabolism	1628:1640	AA catabolism	1628:1640	A reduction in hepatic enzymes related to AA catabolism was also observed during fructose feeding (compared with PER), whereas glycogen concentrations were enhanced in both intestine (+300%) and muscle (+21%).
29378055	4	47	dep	PER	896:898	arg1	g/d					904:906	10 g/d	901:906	10 g/d	901:906	Methods For 45 d, 22-mo-old male Wistar rats (average weight: 716 g) were fed a control ration (13% protein) either at normal (20 g/d), restricted (PER: 10 g/d), or at PER levels supplemented with glucose (3 g/d) or fructose (3 g/d) and then studied in the postabsorptive state.
29378055	11	48	from	advantageous	2027:2038	arg1	proteins					2056:2063	sparing body proteins	2043:2063	sparing body proteins	2043:2063	This could be advantageous in sparing body proteins, particularly during catabolic states, such as those related to malnutrition during aging.
29378055	8	49	theme	circulating	1457:1467	arg1	concentrations					1487:1500	circulating branched-chain AA concentrations	1457:1500	circulating branched-chain AA concentrations	1457:1500	Fructose feeding also reduced circulating branched-chain AA concentrations by 50% (compared with PER) and increased those of alanine (+65% compared with PER).
29378055	10	50	dep	Conclusions	1796:1806	arg1	showed					1811:1816	showed	1811:1816	showed that in PER older rats, fructose feeding improved body composition and the weight of several organs by reducing AA catabolism and utilization for energy production and liver autophagy potential	1811:2010	Conclusions We showed that in PER older rats, fructose feeding improved body composition and the weight of several organs by reducing AA catabolism and utilization for energy production and liver autophagy potential.
29378055	8	51	theme	AA	1484:1485	arg1	concentrations					1487:1500	circulating branched-chain AA concentrations	1457:1500	circulating branched-chain AA concentrations	1457:1500	Fructose feeding also reduced circulating branched-chain AA concentrations by 50% (compared with PER) and increased those of alanine (+65% compared with PER).
29378055	1	52	theme	high	178:181	arg1	intake					190:195	high energy intake	178:195	high energy intake	178:195	Background Fructose feeding in the context of high energy intake is recognized as being responsible for metabolic dysregulation.
29378055	10	53	theme	autophagy	1992:2000	arg1	potential					2002:2010	liver autophagy potential	1986:2010	liver autophagy potential	1986:2010	Conclusions We showed that in PER older rats, fructose feeding improved body composition and the weight of several organs by reducing AA catabolism and utilization for energy production and liver autophagy potential.
29378055	6	54	dep	mass	1317:1320	arg1	%					1326:1326	-28%	1323:1326	-28% compared with PER	1323:1344	Results Both glucose and fructose feeding reduced PER-induced loss of BW and lean mass (-27% compared with PER), but only fructose reduced the loss of fat mass (-28% compared with PER).
29378055	6	55	theme	fat	1313:1315	arg1	mass					1317:1320	fat mass	1313:1320	fat mass (-28% compared with PER)	1313:1345	Results Both glucose and fructose feeding reduced PER-induced loss of BW and lean mass (-27% compared with PER), but only fructose reduced the loss of fat mass (-28% compared with PER).
29378055	1	56	theme	intake	190:195	arg1	context					167:173	the context	163:173	the context of high energy intake	163:195	Background Fructose feeding in the context of high energy intake is recognized as being responsible for metabolic dysregulation.
29378055	9	57	theme	fructose	1667:1674	arg1	feeding					1676:1682	fructose feeding	1667:1682	fructose feeding (compared with PER)	1667:1702	A reduction in hepatic enzymes related to AA catabolism was also observed during fructose feeding (compared with PER), whereas glycogen concentrations were enhanced in both intestine (+300%) and muscle (+21%).
29378055	7	58	theme	mass	1421:1424	arg1	loss					1391:1394	the PER-induced loss	1375:1394	the PER-induced loss of muscle and intestinal mass	1375:1424	Fructose feeding prevented the PER-induced loss of muscle and intestinal mass.
29378055	4	59	theme	%	846:846	arg1	protein					848:854	13% protein	844:854	13% protein	844:854	Methods For 45 d, 22-mo-old male Wistar rats (average weight: 716 g) were fed a control ration (13% protein) either at normal (20 g/d), restricted (PER: 10 g/d), or at PER levels supplemented with glucose (3 g/d) or fructose (3 g/d) and then studied in the postabsorptive state.
29378055	4	59	theme	%	846:846	arg1	ration					836:841	a control ration	826:841	a control ration (13% protein)	826:855	Methods For 45 d, 22-mo-old male Wistar rats (average weight: 716 g) were fed a control ration (13% protein) either at normal (20 g/d), restricted (PER: 10 g/d), or at PER levels supplemented with glucose (3 g/d) or fructose (3 g/d) and then studied in the postabsorptive state.
29378055	0	60	from	Nitrogen	84:91	arg1	Rats					126:129	Protein-Energy-Restricted Old Rats	96:129	Protein-Energy-Restricted Old Rats	96:129	Fructose Feeding during the Postabsorptive State Alters Body Composition and Spares Nitrogen in Protein-Energy-Restricted Old Rats.
29378055	10	61	theme	older	1830:1834	arg1	rats					1836:1839	PER older rats	1826:1839	PER older rats	1826:1839	Conclusions We showed that in PER older rats, fructose feeding improved body composition and the weight of several organs by reducing AA catabolism and utilization for energy production and liver autophagy potential.
29378055	4	62	dep	rats	788:791	arg1	weight					802:807	average weight	794:807	average weight: 716 g	794:814	Methods For 45 d, 22-mo-old male Wistar rats (average weight: 716 g) were fed a control ration (13% protein) either at normal (20 g/d), restricted (PER: 10 g/d), or at PER levels supplemented with glucose (3 g/d) or fructose (3 g/d) and then studied in the postabsorptive state.
29378055	7	63	theme	PER-induced	1379:1389	arg1	loss					1391:1394	the PER-induced loss	1375:1394	the PER-induced loss of muscle and intestinal mass	1375:1424	Fructose feeding prevented the PER-induced loss of muscle and intestinal mass.
29378055	4	64	theme	PER	916:918	arg1	levels					920:925	PER levels	916:925	PER levels supplemented with glucose (3 g/d) or fructose (3 g/d)	916:979	Methods For 45 d, 22-mo-old male Wistar rats (average weight: 716 g) were fed a control ration (13% protein) either at normal (20 g/d), restricted (PER: 10 g/d), or at PER levels supplemented with glucose (3 g/d) or fructose (3 g/d) and then studied in the postabsorptive state.
29378055	10	65	theme	body	1868:1871	arg1	composition					1873:1883	body composition	1868:1883	body composition	1868:1883	Conclusions We showed that in PER older rats, fructose feeding improved body composition and the weight of several organs by reducing AA catabolism and utilization for energy production and liver autophagy potential.
29378055	6	66	theme	fructose	1187:1194	arg1	feeding					1196:1202	fructose feeding	1187:1202	fructose feeding	1187:1202	Results Both glucose and fructose feeding reduced PER-induced loss of BW and lean mass (-27% compared with PER), but only fructose reduced the loss of fat mass (-28% compared with PER).
29378055	6	66	theme	fructose	1187:1194	arg1	Results					1162:1168	Results Both glucose and fructose feeding	1162:1202	Results Both glucose and fructose feeding	1162:1202	Results Both glucose and fructose feeding reduced PER-induced loss of BW and lean mass (-27% compared with PER), but only fructose reduced the loss of fat mass (-28% compared with PER).
29378055	4	67	theme	control	828:834	arg1	protein					848:854	13% protein	844:854	13% protein	844:854	Methods For 45 d, 22-mo-old male Wistar rats (average weight: 716 g) were fed a control ration (13% protein) either at normal (20 g/d), restricted (PER: 10 g/d), or at PER levels supplemented with glucose (3 g/d) or fructose (3 g/d) and then studied in the postabsorptive state.
29378055	4	67	theme	control	828:834	arg1	ration					836:841	a control ration	826:841	a control ration (13% protein)	826:855	Methods For 45 d, 22-mo-old male Wistar rats (average weight: 716 g) were fed a control ration (13% protein) either at normal (20 g/d), restricted (PER: 10 g/d), or at PER levels supplemented with glucose (3 g/d) or fructose (3 g/d) and then studied in the postabsorptive state.
29378055	1	68	theme	Fructose	143:150	arg1	feeding					152:158	Background Fructose feeding	132:158	Background Fructose feeding in the context of high energy intake	132:195	Background Fructose feeding in the context of high energy intake is recognized as being responsible for metabolic dysregulation.
29378055	7	69	theme	Fructose	1348:1355	arg1	feeding					1357:1363	Fructose feeding	1348:1363	Fructose feeding	1348:1363	Fructose feeding prevented the PER-induced loss of muscle and intestinal mass.
29378055	11	70	theme	sparing	2043:2049	arg1	proteins					2056:2063	sparing body proteins	2043:2063	sparing body proteins	2043:2063	This could be advantageous in sparing body proteins, particularly during catabolic states, such as those related to malnutrition during aging.
29378055	5	71	theme	enzyme	1065:1070	arg1	activities					1072:1081	enzyme activities	1065:1081	enzyme activities	1065:1081	We measured BW, body composition, and enzyme activities and metabolite concentrations related to glucose, fructose, and AA metabolism.
29378055	9	72	dep	feeding	1676:1682	arg1	compared					1685:1692	compared	1685:1692	compared with PER	1685:1701	A reduction in hepatic enzymes related to AA catabolism was also observed during fructose feeding (compared with PER), whereas glycogen concentrations were enhanced in both intestine (+300%) and muscle (+21%).
29378055	3	73	dep	restriction	588:598	arg1	PER					601:603	PER	601:603	PER	601:603	Objective We hypothesized that fructose feeding during a catabolic situation corresponding to protein-energy restriction (PER) in older rats would reduce AA utilization for energy purposes, thus slowing down the loss of body weight (BW) and improving body composition.
29378055	0	74	theme	Protein-Energy-Restricted	96:120	arg1	Rats					126:129	Protein-Energy-Restricted Old Rats	96:129	Protein-Energy-Restricted Old Rats	96:129	Fructose Feeding during the Postabsorptive State Alters Body Composition and Spares Nitrogen in Protein-Energy-Restricted Old Rats.
29378055	10	75	theme	several	1903:1909	arg1	organs					1911:1916	several organs	1903:1916	several organs	1903:1916	Conclusions We showed that in PER older rats, fructose feeding improved body composition and the weight of several organs by reducing AA catabolism and utilization for energy production and liver autophagy potential.
29378055	5	76	theme	AA	1147:1148	arg1	metabolism					1150:1159	AA metabolism	1147:1159	AA metabolism	1147:1159	We measured BW, body composition, and enzyme activities and metabolite concentrations related to glucose, fructose, and AA metabolism.
29378055	1	77	from	feeding	152:158	arg1	context					167:173	the context	163:173	the context of high energy intake	163:195	Background Fructose feeding in the context of high energy intake is recognized as being responsible for metabolic dysregulation.
29378055	11	78	theme	catabolic	2086:2094	arg1	states					2096:2101	catabolic states	2086:2101	catabolic states	2086:2101	This could be advantageous in sparing body proteins, particularly during catabolic states, such as those related to malnutrition during aging.
29378055	11	78	theme	catabolic	2086:2094	arg1	those					2112:2116	those	2112:2116	those	2112:2116	This could be advantageous in sparing body proteins, particularly during catabolic states, such as those related to malnutrition during aging.
29378055	10	79	theme	AA	1930:1931	arg1	catabolism					1933:1942	AA catabolism	1930:1942	AA catabolism	1930:1942	Conclusions We showed that in PER older rats, fructose feeding improved body composition and the weight of several organs by reducing AA catabolism and utilization for energy production and liver autophagy potential.
29378055	3	80	theme	energy	652:657	arg1	purposes					659:666	energy purposes	652:666	energy purposes	652:666	Objective We hypothesized that fructose feeding during a catabolic situation corresponding to protein-energy restriction (PER) in older rats would reduce AA utilization for energy purposes, thus slowing down the loss of body weight (BW) and improving body composition.
29378055	2	81	theme	nitrogen	408:415	arg1	resources					417:425	spare nitrogen resources	402:425	spare nitrogen resources	402:425	However, its consumption in the postabsorptive state might contribute to reducing the use of amino acids (AAs) as energy substrates and thus spare nitrogen resources, which could be beneficial during catabolic states.
29378055	6	82	theme	lean	1239:1242	arg1	mass					1244:1247	lean mass	1239:1247	lean mass (-27% compared with PER)	1239:1272	Results Both glucose and fructose feeding reduced PER-induced loss of BW and lean mass (-27% compared with PER), but only fructose reduced the loss of fat mass (-28% compared with PER).
29378055	2	83	theme	catabolic	461:469	arg1	states					471:476	catabolic states	461:476	catabolic states	461:476	However, its consumption in the postabsorptive state might contribute to reducing the use of amino acids (AAs) as energy substrates and thus spare nitrogen resources, which could be beneficial during catabolic states.
29378055	4	84	theme	Wistar	781:786	arg1	rats					788:791	22-mo-old male Wistar rats	766:791	22-mo-old male Wistar rats (average weight: 716 g)	766:815	Methods For 45 d, 22-mo-old male Wistar rats (average weight: 716 g) were fed a control ration (13% protein) either at normal (20 g/d), restricted (PER: 10 g/d), or at PER levels supplemented with glucose (3 g/d) or fructose (3 g/d) and then studied in the postabsorptive state.
29378055	0	85	theme	Body	56:59	arg1	Composition					61:71	Body Composition	56:71	Body Composition	56:71	Fructose Feeding during the Postabsorptive State Alters Body Composition and Spares Nitrogen in Protein-Energy-Restricted Old Rats.
29378055	3	86	theme	fructose	510:517	arg1	feeding					519:525	fructose feeding	510:525	fructose feeding during a catabolic situation corresponding to protein-energy restriction (PER) in older rats	510:618	Objective We hypothesized that fructose feeding during a catabolic situation corresponding to protein-energy restriction (PER) in older rats would reduce AA utilization for energy purposes, thus slowing down the loss of body weight (BW) and improving body composition.
29378055	4	87	theme	postabsorptive	1005:1018	arg1	state					1020:1024	the postabsorptive state	1001:1024	the postabsorptive state	1001:1024	Methods For 45 d, 22-mo-old male Wistar rats (average weight: 716 g) were fed a control ration (13% protein) either at normal (20 g/d), restricted (PER: 10 g/d), or at PER levels supplemented with glucose (3 g/d) or fructose (3 g/d) and then studied in the postabsorptive state.
29378055	1	88	theme	metabolic	236:244	arg1	dysregulation					246:258	metabolic dysregulation	236:258	metabolic dysregulation	236:258	Background Fructose feeding in the context of high energy intake is recognized as being responsible for metabolic dysregulation.
29378055	8	89	dep	increased	1533:1541	arg1	%					1564:1564	+65%	1561:1564	+65% compared with PER	1561:1582	Fructose feeding also reduced circulating branched-chain AA concentrations by 50% (compared with PER) and increased those of alanine (+65% compared with PER).
29378055	4	90	theme	22-mo-old	766:774	arg1	rats					788:791	22-mo-old male Wistar rats	766:791	22-mo-old male Wistar rats (average weight: 716 g)	766:815	Methods For 45 d, 22-mo-old male Wistar rats (average weight: 716 g) were fed a control ration (13% protein) either at normal (20 g/d), restricted (PER: 10 g/d), or at PER levels supplemented with glucose (3 g/d) or fructose (3 g/d) and then studied in the postabsorptive state.
31781999	0	0	theme	BCG-Induced	65:75	arg1	Granulomatosis					77:90	BCG-Induced Granulomatosis	65:90	BCG-Induced Granulomatosis after Administration of Liposome-Encapsulated Composition of Oxidized Dextran and Isonicotinic Acid Hydrazide	65:200	Remodeling of the Extracellular Matrix of the Liver in Mice with BCG-Induced Granulomatosis after Administration of Liposome-Encapsulated Composition of Oxidized Dextran and Isonicotinic Acid Hydrazide.
31781999	2	1	theme	form	704:707	arg1	effect					653:658	The antifibrotic effect	636:658	The antifibrotic effect of dextrazide and its liposome-encapsulated form	636:707	The antifibrotic effect of dextrazide and its liposome-encapsulated form manifested in reduction of hydroxyproline fractions that reflected the process of collagen synthesis.
31781999	2	2	theme	antifibrotic	640:651	arg1	effect					653:658	The antifibrotic effect	636:658	The antifibrotic effect of dextrazide and its liposome-encapsulated form	636:707	The antifibrotic effect of dextrazide and its liposome-encapsulated form manifested in reduction of hydroxyproline fractions that reflected the process of collagen synthesis.
31781999	1	3	theme	oxidized	378:385	arg1	dextran					387:393	oxidized dextran	378:393	oxidized dextran conjugated with isonicotinic acid hydrazide, dextrazide, and its liposome-encapsulated form manifested in modification of the proteoglycan composition	378:544	Remodeling of the extracellular matrix of the liver in mice with BCG-induced granulomatosis after 2-month course of intraperitoneal injections of isonicotinic acid hydrazide, oxidized dextran conjugated with isonicotinic acid hydrazide, dextrazide, and its liposome-encapsulated form manifested in modification of the proteoglycan composition and hydroxyproline fractions associated with changes in hyaluronidase and MMP activities.
31781999	0	4	theme	Hydrazide	192:200	arg1	Composition					138:148	Liposome-Encapsulated Composition	116:148	Liposome-Encapsulated Composition of Oxidized Dextran and Isonicotinic Acid Hydrazide	116:200	Remodeling of the Extracellular Matrix of the Liver in Mice with BCG-Induced Granulomatosis after Administration of Liposome-Encapsulated Composition of Oxidized Dextran and Isonicotinic Acid Hydrazide.
31781999	1	5	theme	BCG-induced	268:278	arg1	granulomatosis					280:293	BCG-induced granulomatosis	268:293	BCG-induced granulomatosis after 2-month course of intraperitoneal injections of isonicotinic acid hydrazide	268:375	Remodeling of the extracellular matrix of the liver in mice with BCG-induced granulomatosis after 2-month course of intraperitoneal injections of isonicotinic acid hydrazide, oxidized dextran conjugated with isonicotinic acid hydrazide, dextrazide, and its liposome-encapsulated form manifested in modification of the proteoglycan composition and hydroxyproline fractions associated with changes in hyaluronidase and MMP activities.
31781999	0	6	from	Remodeling	0:9	arg1	Mice					55:58	Mice	55:58	Mice with BCG-Induced Granulomatosis after Administration of Liposome-Encapsulated Composition of Oxidized Dextran and Isonicotinic Acid Hydrazide	55:200	Remodeling of the Extracellular Matrix of the Liver in Mice with BCG-Induced Granulomatosis after Administration of Liposome-Encapsulated Composition of Oxidized Dextran and Isonicotinic Acid Hydrazide.
31781999	2	7	theme	fractions	751:759	arg1	reduction					723:731	reduction	723:731	reduction of hydroxyproline fractions that reflected the process of collagen synthesis	723:808	The antifibrotic effect of dextrazide and its liposome-encapsulated form manifested in reduction of hydroxyproline fractions that reflected the process of collagen synthesis.
31781999	3	8	theme	hydrazide	847:855	arg1	Administration					811:824	Administration	811:824	Administration of isonicotinic acid hydrazide	811:855	Administration of isonicotinic acid hydrazide was followed by a decrease in hydroxyproline fractions reflecting collagen synthesis (antifibrotics effect) and degradation (profibrotic effect), which can be explained by its hepatotoxic activity.
31781999	0	9	with	Mice	55:58	arg1	Granulomatosis					77:90	BCG-Induced Granulomatosis	65:90	BCG-Induced Granulomatosis after Administration of Liposome-Encapsulated Composition of Oxidized Dextran and Isonicotinic Acid Hydrazide	65:200	Remodeling of the Extracellular Matrix of the Liver in Mice with BCG-Induced Granulomatosis after Administration of Liposome-Encapsulated Composition of Oxidized Dextran and Isonicotinic Acid Hydrazide.
31781999	3	10	theme	acid	842:845	arg1	hydrazide					847:855	isonicotinic acid hydrazide	829:855	isonicotinic acid hydrazide	829:855	Administration of isonicotinic acid hydrazide was followed by a decrease in hydroxyproline fractions reflecting collagen synthesis (antifibrotics effect) and degradation (profibrotic effect), which can be explained by its hepatotoxic activity.
31781999	1	11	theme	MMP	620:622	arg1	activities					624:633	MMP activities	620:633	MMP activities	620:633	Remodeling of the extracellular matrix of the liver in mice with BCG-induced granulomatosis after 2-month course of intraperitoneal injections of isonicotinic acid hydrazide, oxidized dextran conjugated with isonicotinic acid hydrazide, dextrazide, and its liposome-encapsulated form manifested in modification of the proteoglycan composition and hydroxyproline fractions associated with changes in hyaluronidase and MMP activities.
31781999	2	12	theme	hydroxyproline	736:749	arg1	fractions					751:759	hydroxyproline fractions	736:759	hydroxyproline fractions	736:759	The antifibrotic effect of dextrazide and its liposome-encapsulated form manifested in reduction of hydroxyproline fractions that reflected the process of collagen synthesis.
31781999	3	13	theme	hepatotoxic	1033:1043	arg1	activity					1045:1052	its hepatotoxic activity	1029:1052	its hepatotoxic activity	1029:1052	Administration of isonicotinic acid hydrazide was followed by a decrease in hydroxyproline fractions reflecting collagen synthesis (antifibrotics effect) and degradation (profibrotic effect), which can be explained by its hepatotoxic activity.
31781999	1	14	theme	isonicotinic	411:422	arg1	hydrazide					429:437	isonicotinic acid hydrazide	411:437	isonicotinic acid hydrazide	411:437	Remodeling of the extracellular matrix of the liver in mice with BCG-induced granulomatosis after 2-month course of intraperitoneal injections of isonicotinic acid hydrazide, oxidized dextran conjugated with isonicotinic acid hydrazide, dextrazide, and its liposome-encapsulated form manifested in modification of the proteoglycan composition and hydroxyproline fractions associated with changes in hyaluronidase and MMP activities.
31781999	2	15	theme	collagen	791:798	arg1	synthesis					800:808	collagen synthesis	791:808	collagen synthesis	791:808	The antifibrotic effect of dextrazide and its liposome-encapsulated form manifested in reduction of hydroxyproline fractions that reflected the process of collagen synthesis.
31781999	3	16	theme	antifibrotics	943:955	arg1	synthesis					932:940	collagen synthesis	923:940	collagen synthesis (antifibrotics effect)	923:963	Administration of isonicotinic acid hydrazide was followed by a decrease in hydroxyproline fractions reflecting collagen synthesis (antifibrotics effect) and degradation (profibrotic effect), which can be explained by its hepatotoxic activity.
31781999	3	16	theme	antifibrotics	943:955	arg1	effect					957:962	antifibrotics effect	943:962	antifibrotics effect	943:962	Administration of isonicotinic acid hydrazide was followed by a decrease in hydroxyproline fractions reflecting collagen synthesis (antifibrotics effect) and degradation (profibrotic effect), which can be explained by its hepatotoxic activity.
31781999	1	17	theme	acid	424:427	arg1	hydrazide					429:437	isonicotinic acid hydrazide	411:437	isonicotinic acid hydrazide	411:437	Remodeling of the extracellular matrix of the liver in mice with BCG-induced granulomatosis after 2-month course of intraperitoneal injections of isonicotinic acid hydrazide, oxidized dextran conjugated with isonicotinic acid hydrazide, dextrazide, and its liposome-encapsulated form manifested in modification of the proteoglycan composition and hydroxyproline fractions associated with changes in hyaluronidase and MMP activities.
31781999	1	18	theme	proteoglycan	521:532	arg1	composition					534:544	the proteoglycan composition	517:544	the proteoglycan composition	517:544	Remodeling of the extracellular matrix of the liver in mice with BCG-induced granulomatosis after 2-month course of intraperitoneal injections of isonicotinic acid hydrazide, oxidized dextran conjugated with isonicotinic acid hydrazide, dextrazide, and its liposome-encapsulated form manifested in modification of the proteoglycan composition and hydroxyproline fractions associated with changes in hyaluronidase and MMP activities.
31781999	1	19	from	changes	591:597	arg1	hyaluronidase					602:614	hyaluronidase	602:614	hyaluronidase	602:614	Remodeling of the extracellular matrix of the liver in mice with BCG-induced granulomatosis after 2-month course of intraperitoneal injections of isonicotinic acid hydrazide, oxidized dextran conjugated with isonicotinic acid hydrazide, dextrazide, and its liposome-encapsulated form manifested in modification of the proteoglycan composition and hydroxyproline fractions associated with changes in hyaluronidase and MMP activities.
31781999	1	19	from	changes	591:597	arg1	activities					624:633	MMP activities	620:633	MMP activities	620:633	Remodeling of the extracellular matrix of the liver in mice with BCG-induced granulomatosis after 2-month course of intraperitoneal injections of isonicotinic acid hydrazide, oxidized dextran conjugated with isonicotinic acid hydrazide, dextrazide, and its liposome-encapsulated form manifested in modification of the proteoglycan composition and hydroxyproline fractions associated with changes in hyaluronidase and MMP activities.
31781999	1	20	theme	2-month	301:307	arg1	course					309:314	2-month course	301:314	2-month course of intraperitoneal injections of isonicotinic acid hydrazide	301:375	Remodeling of the extracellular matrix of the liver in mice with BCG-induced granulomatosis after 2-month course of intraperitoneal injections of isonicotinic acid hydrazide, oxidized dextran conjugated with isonicotinic acid hydrazide, dextrazide, and its liposome-encapsulated form manifested in modification of the proteoglycan composition and hydroxyproline fractions associated with changes in hyaluronidase and MMP activities.
31781999	3	21	from	decrease	875:882	arg1	fractions					902:910	hydroxyproline fractions	887:910	hydroxyproline fractions reflecting collagen synthesis (antifibrotics effect) and degradation (profibrotic effect)	887:1000	Administration of isonicotinic acid hydrazide was followed by a decrease in hydroxyproline fractions reflecting collagen synthesis (antifibrotics effect) and degradation (profibrotic effect), which can be explained by its hepatotoxic activity.
31781999	1	22	theme	composition	534:544	arg1	modification					501:512	modification	501:512	modification of the proteoglycan composition	501:544	Remodeling of the extracellular matrix of the liver in mice with BCG-induced granulomatosis after 2-month course of intraperitoneal injections of isonicotinic acid hydrazide, oxidized dextran conjugated with isonicotinic acid hydrazide, dextrazide, and its liposome-encapsulated form manifested in modification of the proteoglycan composition and hydroxyproline fractions associated with changes in hyaluronidase and MMP activities.
31781999	2	23	theme	liposome-encapsulated	682:702	arg1	form					704:707	its liposome-encapsulated form	678:707	its liposome-encapsulated form	678:707	The antifibrotic effect of dextrazide and its liposome-encapsulated form manifested in reduction of hydroxyproline fractions that reflected the process of collagen synthesis.
31781999	0	24	theme	Extracellular	18:30	arg1	Matrix					32:37	the Extracellular Matrix	14:37	the Extracellular Matrix of the Liver	14:50	Remodeling of the Extracellular Matrix of the Liver in Mice with BCG-Induced Granulomatosis after Administration of Liposome-Encapsulated Composition of Oxidized Dextran and Isonicotinic Acid Hydrazide.
31781999	0	25	theme	Composition	138:148	arg1	Administration					98:111	Administration	98:111	Administration of Liposome-Encapsulated Composition of Oxidized Dextran and Isonicotinic Acid Hydrazide	98:200	Remodeling of the Extracellular Matrix of the Liver in Mice with BCG-Induced Granulomatosis after Administration of Liposome-Encapsulated Composition of Oxidized Dextran and Isonicotinic Acid Hydrazide.
31781999	0	26	theme	Liposome-Encapsulated	116:136	arg1	Composition					138:148	Liposome-Encapsulated Composition	116:148	Liposome-Encapsulated Composition of Oxidized Dextran and Isonicotinic Acid Hydrazide	116:200	Remodeling of the Extracellular Matrix of the Liver in Mice with BCG-Induced Granulomatosis after Administration of Liposome-Encapsulated Composition of Oxidized Dextran and Isonicotinic Acid Hydrazide.
31781999	1	27	theme	extracellular	221:233	arg1	matrix					235:240	the extracellular matrix	217:240	the extracellular matrix of the liver	217:253	Remodeling of the extracellular matrix of the liver in mice with BCG-induced granulomatosis after 2-month course of intraperitoneal injections of isonicotinic acid hydrazide, oxidized dextran conjugated with isonicotinic acid hydrazide, dextrazide, and its liposome-encapsulated form manifested in modification of the proteoglycan composition and hydroxyproline fractions associated with changes in hyaluronidase and MMP activities.
31781999	1	28	theme	intraperitoneal	319:333	arg1	injections					335:344	intraperitoneal injections	319:344	intraperitoneal injections of isonicotinic acid hydrazide	319:375	Remodeling of the extracellular matrix of the liver in mice with BCG-induced granulomatosis after 2-month course of intraperitoneal injections of isonicotinic acid hydrazide, oxidized dextran conjugated with isonicotinic acid hydrazide, dextrazide, and its liposome-encapsulated form manifested in modification of the proteoglycan composition and hydroxyproline fractions associated with changes in hyaluronidase and MMP activities.
31781999	0	29	theme	Oxidized	153:160	arg1	Dextran					162:168	Oxidized Dextran	153:168	Oxidized Dextran	153:168	Remodeling of the Extracellular Matrix of the Liver in Mice with BCG-Induced Granulomatosis after Administration of Liposome-Encapsulated Composition of Oxidized Dextran and Isonicotinic Acid Hydrazide.
31781999	1	30	theme	matrix	235:240	arg1	Remodeling					203:212	Remodeling	203:212	Remodeling of the extracellular matrix of the liver in mice with BCG-induced granulomatosis after 2-month course of intraperitoneal injections of isonicotinic acid hydrazide, oxidized dextran conjugated with isonicotinic acid hydrazide, dextrazide, and its liposome-encapsulated form manifested in modification of the proteoglycan composition and hydroxyproline fractions	203:573	Remodeling of the extracellular matrix of the liver in mice with BCG-induced granulomatosis after 2-month course of intraperitoneal injections of isonicotinic acid hydrazide, oxidized dextran conjugated with isonicotinic acid hydrazide, dextrazide, and its liposome-encapsulated form manifested in modification of the proteoglycan composition and hydroxyproline fractions associated with changes in hyaluronidase and MMP activities.
31781999	0	31	theme	Matrix	32:37	arg1	Remodeling					0:9	Remodeling	0:9	Remodeling of the Extracellular Matrix of the Liver in Mice with BCG-Induced Granulomatosis after Administration of Liposome-Encapsulated Composition of Oxidized Dextran and Isonicotinic Acid Hydrazide.	0:201	Remodeling of the Extracellular Matrix of the Liver in Mice with BCG-Induced Granulomatosis after Administration of Liposome-Encapsulated Composition of Oxidized Dextran and Isonicotinic Acid Hydrazide.
31781999	1	32	theme	injections	335:344	arg1	course					309:314	2-month course	301:314	2-month course of intraperitoneal injections of isonicotinic acid hydrazide	301:375	Remodeling of the extracellular matrix of the liver in mice with BCG-induced granulomatosis after 2-month course of intraperitoneal injections of isonicotinic acid hydrazide, oxidized dextran conjugated with isonicotinic acid hydrazide, dextrazide, and its liposome-encapsulated form manifested in modification of the proteoglycan composition and hydroxyproline fractions associated with changes in hyaluronidase and MMP activities.
31781999	1	33	theme	hydroxyproline	550:563	arg1	fractions					565:573	hydroxyproline fractions	550:573	hydroxyproline fractions	550:573	Remodeling of the extracellular matrix of the liver in mice with BCG-induced granulomatosis after 2-month course of intraperitoneal injections of isonicotinic acid hydrazide, oxidized dextran conjugated with isonicotinic acid hydrazide, dextrazide, and its liposome-encapsulated form manifested in modification of the proteoglycan composition and hydroxyproline fractions associated with changes in hyaluronidase and MMP activities.
31781999	3	34	theme	hydroxyproline	887:900	arg1	fractions					902:910	hydroxyproline fractions	887:910	hydroxyproline fractions reflecting collagen synthesis (antifibrotics effect) and degradation (profibrotic effect)	887:1000	Administration of isonicotinic acid hydrazide was followed by a decrease in hydroxyproline fractions reflecting collagen synthesis (antifibrotics effect) and degradation (profibrotic effect), which can be explained by its hepatotoxic activity.
31781999	0	35	theme	Liver	46:50	arg1	Matrix					32:37	the Extracellular Matrix	14:37	the Extracellular Matrix of the Liver	14:50	Remodeling of the Extracellular Matrix of the Liver in Mice with BCG-Induced Granulomatosis after Administration of Liposome-Encapsulated Composition of Oxidized Dextran and Isonicotinic Acid Hydrazide.
31781999	2	36	theme	dextrazide	663:672	arg1	effect					653:658	The antifibrotic effect	636:658	The antifibrotic effect of dextrazide and its liposome-encapsulated form	636:707	The antifibrotic effect of dextrazide and its liposome-encapsulated form manifested in reduction of hydroxyproline fractions that reflected the process of collagen synthesis.
31781999	3	37	theme	isonicotinic	829:840	arg1	hydrazide					847:855	isonicotinic acid hydrazide	829:855	isonicotinic acid hydrazide	829:855	Administration of isonicotinic acid hydrazide was followed by a decrease in hydroxyproline fractions reflecting collagen synthesis (antifibrotics effect) and degradation (profibrotic effect), which can be explained by its hepatotoxic activity.
31781999	1	38	theme	isonicotinic	349:360	arg1	hydrazide					367:375	isonicotinic acid hydrazide	349:375	isonicotinic acid hydrazide	349:375	Remodeling of the extracellular matrix of the liver in mice with BCG-induced granulomatosis after 2-month course of intraperitoneal injections of isonicotinic acid hydrazide, oxidized dextran conjugated with isonicotinic acid hydrazide, dextrazide, and its liposome-encapsulated form manifested in modification of the proteoglycan composition and hydroxyproline fractions associated with changes in hyaluronidase and MMP activities.
31781999	0	39	theme	Dextran	162:168	arg1	Composition					138:148	Liposome-Encapsulated Composition	116:148	Liposome-Encapsulated Composition of Oxidized Dextran and Isonicotinic Acid Hydrazide	116:200	Remodeling of the Extracellular Matrix of the Liver in Mice with BCG-Induced Granulomatosis after Administration of Liposome-Encapsulated Composition of Oxidized Dextran and Isonicotinic Acid Hydrazide.
31781999	1	40	theme	acid	362:365	arg1	hydrazide					367:375	isonicotinic acid hydrazide	349:375	isonicotinic acid hydrazide	349:375	Remodeling of the extracellular matrix of the liver in mice with BCG-induced granulomatosis after 2-month course of intraperitoneal injections of isonicotinic acid hydrazide, oxidized dextran conjugated with isonicotinic acid hydrazide, dextrazide, and its liposome-encapsulated form manifested in modification of the proteoglycan composition and hydroxyproline fractions associated with changes in hyaluronidase and MMP activities.
31781999	0	41	theme	Acid	187:190	arg1	Hydrazide					192:200	Isonicotinic Acid Hydrazide	174:200	Isonicotinic Acid Hydrazide	174:200	Remodeling of the Extracellular Matrix of the Liver in Mice with BCG-Induced Granulomatosis after Administration of Liposome-Encapsulated Composition of Oxidized Dextran and Isonicotinic Acid Hydrazide.
31781999	2	42	theme	synthesis	800:808	arg1	process					780:786	the process	776:786	the process of collagen synthesis	776:808	The antifibrotic effect of dextrazide and its liposome-encapsulated form manifested in reduction of hydroxyproline fractions that reflected the process of collagen synthesis.
31781999	0	43	theme	Isonicotinic	174:185	arg1	Hydrazide					192:200	Isonicotinic Acid Hydrazide	174:200	Isonicotinic Acid Hydrazide	174:200	Remodeling of the Extracellular Matrix of the Liver in Mice with BCG-Induced Granulomatosis after Administration of Liposome-Encapsulated Composition of Oxidized Dextran and Isonicotinic Acid Hydrazide.
31781999	1	44	theme	liposome-encapsulated	460:480	arg1	form					482:485	its liposome-encapsulated form	456:485	its liposome-encapsulated form	456:485	Remodeling of the extracellular matrix of the liver in mice with BCG-induced granulomatosis after 2-month course of intraperitoneal injections of isonicotinic acid hydrazide, oxidized dextran conjugated with isonicotinic acid hydrazide, dextrazide, and its liposome-encapsulated form manifested in modification of the proteoglycan composition and hydroxyproline fractions associated with changes in hyaluronidase and MMP activities.
31781999	1	45	from	Remodeling	203:212	arg1	mice					258:261	mice	258:261	mice with BCG-induced granulomatosis after 2-month course of intraperitoneal injections of isonicotinic acid hydrazide, oxidized dextran conjugated with isonicotinic acid hydrazide, dextrazide, and its liposome-encapsulated form manifested in modification of the proteoglycan composition and hydroxyproline fractions	258:573	Remodeling of the extracellular matrix of the liver in mice with BCG-induced granulomatosis after 2-month course of intraperitoneal injections of isonicotinic acid hydrazide, oxidized dextran conjugated with isonicotinic acid hydrazide, dextrazide, and its liposome-encapsulated form manifested in modification of the proteoglycan composition and hydroxyproline fractions associated with changes in hyaluronidase and MMP activities.
31781999	1	46	theme	liver	249:253	arg1	matrix					235:240	the extracellular matrix	217:240	the extracellular matrix of the liver	217:253	Remodeling of the extracellular matrix of the liver in mice with BCG-induced granulomatosis after 2-month course of intraperitoneal injections of isonicotinic acid hydrazide, oxidized dextran conjugated with isonicotinic acid hydrazide, dextrazide, and its liposome-encapsulated form manifested in modification of the proteoglycan composition and hydroxyproline fractions associated with changes in hyaluronidase and MMP activities.
31781999	3	47	dep	degradation	969:979	arg1	effect					994:999	profibrotic effect	982:999	degradation (profibrotic effect)	969:1000	Administration of isonicotinic acid hydrazide was followed by a decrease in hydroxyproline fractions reflecting collagen synthesis (antifibrotics effect) and degradation (profibrotic effect), which can be explained by its hepatotoxic activity.
31781999	1	48	with	mice	258:261	arg1	dextran					387:393	oxidized dextran	378:393	oxidized dextran conjugated with isonicotinic acid hydrazide, dextrazide, and its liposome-encapsulated form manifested in modification of the proteoglycan composition	378:544	Remodeling of the extracellular matrix of the liver in mice with BCG-induced granulomatosis after 2-month course of intraperitoneal injections of isonicotinic acid hydrazide, oxidized dextran conjugated with isonicotinic acid hydrazide, dextrazide, and its liposome-encapsulated form manifested in modification of the proteoglycan composition and hydroxyproline fractions associated with changes in hyaluronidase and MMP activities.
31781999	1	48	with	mice	258:261	arg1	granulomatosis					280:293	BCG-induced granulomatosis	268:293	BCG-induced granulomatosis after 2-month course of intraperitoneal injections of isonicotinic acid hydrazide	268:375	Remodeling of the extracellular matrix of the liver in mice with BCG-induced granulomatosis after 2-month course of intraperitoneal injections of isonicotinic acid hydrazide, oxidized dextran conjugated with isonicotinic acid hydrazide, dextrazide, and its liposome-encapsulated form manifested in modification of the proteoglycan composition and hydroxyproline fractions associated with changes in hyaluronidase and MMP activities.
31781999	1	48	with	mice	258:261	arg1	fractions					565:573	hydroxyproline fractions	550:573	hydroxyproline fractions	550:573	Remodeling of the extracellular matrix of the liver in mice with BCG-induced granulomatosis after 2-month course of intraperitoneal injections of isonicotinic acid hydrazide, oxidized dextran conjugated with isonicotinic acid hydrazide, dextrazide, and its liposome-encapsulated form manifested in modification of the proteoglycan composition and hydroxyproline fractions associated with changes in hyaluronidase and MMP activities.
31781999	3	49	theme	profibrotic	982:992	arg1	effect					994:999	profibrotic effect	982:999	degradation (profibrotic effect)	969:1000	Administration of isonicotinic acid hydrazide was followed by a decrease in hydroxyproline fractions reflecting collagen synthesis (antifibrotics effect) and degradation (profibrotic effect), which can be explained by its hepatotoxic activity.
31781999	3	50	theme	collagen	923:930	arg1	synthesis					932:940	collagen synthesis	923:940	collagen synthesis (antifibrotics effect)	923:963	Administration of isonicotinic acid hydrazide was followed by a decrease in hydroxyproline fractions reflecting collagen synthesis (antifibrotics effect) and degradation (profibrotic effect), which can be explained by its hepatotoxic activity.
31781999	3	50	theme	collagen	923:930	arg1	effect					957:962	antifibrotics effect	943:962	antifibrotics effect	943:962	Administration of isonicotinic acid hydrazide was followed by a decrease in hydroxyproline fractions reflecting collagen synthesis (antifibrotics effect) and degradation (profibrotic effect), which can be explained by its hepatotoxic activity.
31781999	1	51	theme	hydrazide	367:375	arg1	injections					335:344	intraperitoneal injections	319:344	intraperitoneal injections of isonicotinic acid hydrazide	319:375	Remodeling of the extracellular matrix of the liver in mice with BCG-induced granulomatosis after 2-month course of intraperitoneal injections of isonicotinic acid hydrazide, oxidized dextran conjugated with isonicotinic acid hydrazide, dextrazide, and its liposome-encapsulated form manifested in modification of the proteoglycan composition and hydroxyproline fractions associated with changes in hyaluronidase and MMP activities.
29482868	5	0	theme	more	1029:1032	arg1	1.7-times					1019:1027	1.7-times	1019:1027	1.7-times more C17:0 iso than GGR1	1019:1052	However, the optimum growth of A7 was at alkaline pH (9.0) and 55°C, compared to pH 7.0 at 60°C for GGR1, and the fatty acid profile of A7 contained 1.7-times more C17:0 iso than GGR1.
29482868	3	1	theme	=	742:742	arg1	101079T					748:754	= DSM 101079T	742:754	= DSM 101079T	742:754	Strain A7 was identified as Herbivorax saccincola by DNA-DNA hybridization, and phylogenetic analysis based on 16S rRNA gene sequences showed that it was closely related to H. saccincola GGR1 (= DSM 101079T).
29482868	3	1	theme	=	742:742	arg1	GGR1					736:739	H. saccincola GGR1	722:739	H. saccincola GGR1 (= DSM 101079T)	722:755	Strain A7 was identified as Herbivorax saccincola by DNA-DNA hybridization, and phylogenetic analysis based on 16S rRNA gene sequences showed that it was closely related to H. saccincola GGR1 (= DSM 101079T).
29482868	3	2	theme	H.	722:723	arg1	101079T					748:754	= DSM 101079T	742:754	= DSM 101079T	742:754	Strain A7 was identified as Herbivorax saccincola by DNA-DNA hybridization, and phylogenetic analysis based on 16S rRNA gene sequences showed that it was closely related to H. saccincola GGR1 (= DSM 101079T).
29482868	3	2	theme	H.	722:723	arg1	GGR1					736:739	H. saccincola GGR1	722:739	H. saccincola GGR1 (= DSM 101079T)	722:755	Strain A7 was identified as Herbivorax saccincola by DNA-DNA hybridization, and phylogenetic analysis based on 16S rRNA gene sequences showed that it was closely related to H. saccincola GGR1 (= DSM 101079T).
29482868	3	3	theme	saccincola	725:734	arg1	101079T					748:754	= DSM 101079T	742:754	= DSM 101079T	742:754	Strain A7 was identified as Herbivorax saccincola by DNA-DNA hybridization, and phylogenetic analysis based on 16S rRNA gene sequences showed that it was closely related to H. saccincola GGR1 (= DSM 101079T).
29482868	3	3	theme	saccincola	725:734	arg1	GGR1					736:739	H. saccincola GGR1	722:739	H. saccincola GGR1 (= DSM 101079T)	722:755	Strain A7 was identified as Herbivorax saccincola by DNA-DNA hybridization, and phylogenetic analysis based on 16S rRNA gene sequences showed that it was closely related to H. saccincola GGR1 (= DSM 101079T).
29482868	5	4	theme	optimum	883:889	arg1	growth					891:896	the optimum growth	879:896	the optimum growth of A7	879:902	However, the optimum growth of A7 was at alkaline pH (9.0) and 55°C, compared to pH 7.0 at 60°C for GGR1, and the fatty acid profile of A7 contained 1.7-times more C17:0 iso than GGR1.
29482868	5	5	theme	C17:0	1034:1038	arg1	iso					1040:1042	C17:0 iso	1034:1042	C17:0 iso	1034:1042	However, the optimum growth of A7 was at alkaline pH (9.0) and 55°C, compared to pH 7.0 at 60°C for GGR1, and the fatty acid profile of A7 contained 1.7-times more C17:0 iso than GGR1.
29482868	2	6	theme	orange	516:521	arg1	colonies					523:530	orange colonies	516:530	orange colonies	516:530	The strain was Gram-positive, non-endospore forming, non-motile, and formed orange colonies on solid medium.
29482868	2	7	theme	solid	535:539	arg1	medium					541:546	solid medium	535:546	solid medium	535:546	The strain was Gram-positive, non-endospore forming, non-motile, and formed orange colonies on solid medium.
29482868	7	8	theme	enzymes	1383:1389	arg1	diversity					1358:1366	more glycoside hydrolases (GHs) belonging to GH families-11 and -2, and more diversity	1281:1366	more glycoside hydrolases (GHs) belonging to GH families-11 and -2, and more diversity of xylanolytic enzymes	1281:1389	H. saccincola A7 contained more glycoside hydrolases (GHs) belonging to GH families-11 and -2, and more diversity of xylanolytic enzymes, than C. thermocellum and C. clariflavum.
29482868	1	9	from	compost	325:331	arg1	Island					345:350	Ishigaki Island	336:350	Ishigaki Island	336:350	An anaerobic, cellulolytic-xylanolytic bacterium, designated strain A7, was isolated from a cellulose-degrading bacterial community inhabiting bovine manure compost on Ishigaki Island, Japan, by enrichment culture using unpretreated corn stover as the sole carbon source.
29482868	7	10	theme	glycoside	1286:1294	arg1	hydrolases					1296:1305	glycoside hydrolases	1286:1305	glycoside hydrolases (GHs) belonging to GH families-11 and -2	1286:1346	H. saccincola A7 contained more glycoside hydrolases (GHs) belonging to GH families-11 and -2, and more diversity of xylanolytic enzymes, than C. thermocellum and C. clariflavum.
29482868	7	10	theme	glycoside	1286:1294	arg1	GHs					1308:1310	GHs	1308:1310	GHs	1308:1310	H. saccincola A7 contained more glycoside hydrolases (GHs) belonging to GH families-11 and -2, and more diversity of xylanolytic enzymes, than C. thermocellum and C. clariflavum.
29482868	3	11	theme	Strain	549:554	arg1	saccincola					588:597	Herbivorax saccincola	577:597	Herbivorax saccincola	577:597	Strain A7 was identified as Herbivorax saccincola by DNA-DNA hybridization, and phylogenetic analysis based on 16S rRNA gene sequences showed that it was closely related to H. saccincola GGR1 (= DSM 101079T).
29482868	3	11	theme	Strain	549:554	arg1	A7					556:557	Strain A7	549:557	Strain A7	549:557	Strain A7 was identified as Herbivorax saccincola by DNA-DNA hybridization, and phylogenetic analysis based on 16S rRNA gene sequences showed that it was closely related to H. saccincola GGR1 (= DSM 101079T).
29482868	1	12	theme	cellulose-degrading	260:278	arg1	community					290:298	a cellulose-degrading bacterial community	258:298	a cellulose-degrading bacterial community inhabiting bovine manure compost on Ishigaki Island	258:350	An anaerobic, cellulolytic-xylanolytic bacterium, designated strain A7, was isolated from a cellulose-degrading bacterial community inhabiting bovine manure compost on Ishigaki Island, Japan, by enrichment culture using unpretreated corn stover as the sole carbon source.
29482868	1	12	theme	cellulose-degrading	260:278	arg1	Japan					353:357	Japan	353:357	Japan	353:357	An anaerobic, cellulolytic-xylanolytic bacterium, designated strain A7, was isolated from a cellulose-degrading bacterial community inhabiting bovine manure compost on Ishigaki Island, Japan, by enrichment culture using unpretreated corn stover as the sole carbon source.
29482868	0	13	theme	anaerobic	88:96	arg1	bacterium					157:165	an anaerobic, alkaliphilic, thermophilic, cellulolytic, and xylanolytic bacterium	85:165	an anaerobic, alkaliphilic, thermophilic, cellulolytic, and xylanolytic bacterium	85:165	Characterization and high-quality draft genome sequence of Herbivorax saccincola A7, an anaerobic, alkaliphilic, thermophilic, cellulolytic, and xylanolytic bacterium.
29482868	0	13	theme	anaerobic	88:96	arg1	A7					81:82	A7	81:82	A7	81:82	Characterization and high-quality draft genome sequence of Herbivorax saccincola A7, an anaerobic, alkaliphilic, thermophilic, cellulolytic, and xylanolytic bacterium.
29482868	1	14	theme	enrichment	363:372	arg1	culture					374:380	enrichment culture	363:380	enrichment culture using unpretreated corn stover as the sole carbon source	363:437	An anaerobic, cellulolytic-xylanolytic bacterium, designated strain A7, was isolated from a cellulose-degrading bacterial community inhabiting bovine manure compost on Ishigaki Island, Japan, by enrichment culture using unpretreated corn stover as the sole carbon source.
29482868	2	15	dep	forming	484:490	arg1	non-endospore					470:482	non-endospore	470:482	non-endospore	470:482	The strain was Gram-positive, non-endospore forming, non-motile, and formed orange colonies on solid medium.
29482868	1	16	theme	bacterial	280:288	arg1	community					290:298	a cellulose-degrading bacterial community	258:298	a cellulose-degrading bacterial community inhabiting bovine manure compost on Ishigaki Island	258:350	An anaerobic, cellulolytic-xylanolytic bacterium, designated strain A7, was isolated from a cellulose-degrading bacterial community inhabiting bovine manure compost on Ishigaki Island, Japan, by enrichment culture using unpretreated corn stover as the sole carbon source.
29482868	1	16	theme	bacterial	280:288	arg1	Japan					353:357	Japan	353:357	Japan	353:357	An anaerobic, cellulolytic-xylanolytic bacterium, designated strain A7, was isolated from a cellulose-degrading bacterial community inhabiting bovine manure compost on Ishigaki Island, Japan, by enrichment culture using unpretreated corn stover as the sole carbon source.
29482868	8	17	from	thermocellum	1651:1662	arg1	transporter					1545:1555	a xylose transporter	1536:1555	a xylose transporter	1536:1555	H. saccincola A7 could grow on xylan because it encoded essential genes for xylose metabolism, such as a xylose transporter, xylose isomerase, xylulokinase, and ribulose-phosphate 3-epimerase, which are absent from C. thermocellum.
29482868	8	17	from	thermocellum	1651:1662	arg1	absent					1636:1641	absent	1636:1641	absent	1636:1641	H. saccincola A7 could grow on xylan because it encoded essential genes for xylose metabolism, such as a xylose transporter, xylose isomerase, xylulokinase, and ribulose-phosphate 3-epimerase, which are absent from C. thermocellum.
29482868	4	18	theme	similar	810:816	arg1	characteristics					829:843	quite similar phenotypic characteristics	804:843	quite similar phenotypic characteristics	804:843	H. saccincola A7 (= JCM 31827=DSM 104321) had quite similar phenotypic characteristics to those of strain GGR1.
29482868	8	19	theme	xylose	1558:1563	arg1	isomerase					1565:1573	xylose isomerase	1558:1573	xylose isomerase	1558:1573	H. saccincola A7 could grow on xylan because it encoded essential genes for xylose metabolism, such as a xylose transporter, xylose isomerase, xylulokinase, and ribulose-phosphate 3-epimerase, which are absent from C. thermocellum.
29482868	6	20	theme	genome	1065:1070	arg1	sequence					1072:1079	The draft genome sequence	1055:1079	The draft genome sequence	1055:1079	The draft genome sequence revealed that H. saccincola A7 possessed a cellulosome-like extracellular macromolecular complex, which has also been found for Clostridium thermocellum and C. clariflavum.
29482868	6	21	theme	macromolecular	1155:1168	arg1	complex					1170:1176	a cellulosome-like extracellular macromolecular complex	1122:1176	a cellulosome-like extracellular macromolecular complex	1122:1176	The draft genome sequence revealed that H. saccincola A7 possessed a cellulosome-like extracellular macromolecular complex, which has also been found for Clostridium thermocellum and C. clariflavum.
29482868	0	22	theme	alkaliphilic	99:110	arg1	bacterium					157:165	an anaerobic, alkaliphilic, thermophilic, cellulolytic, and xylanolytic bacterium	85:165	an anaerobic, alkaliphilic, thermophilic, cellulolytic, and xylanolytic bacterium	85:165	Characterization and high-quality draft genome sequence of Herbivorax saccincola A7, an anaerobic, alkaliphilic, thermophilic, cellulolytic, and xylanolytic bacterium.
29482868	0	22	theme	alkaliphilic	99:110	arg1	A7					81:82	A7	81:82	A7	81:82	Characterization and high-quality draft genome sequence of Herbivorax saccincola A7, an anaerobic, alkaliphilic, thermophilic, cellulolytic, and xylanolytic bacterium.
29482868	5	23	theme	A7	1006:1007	arg1	profile					995:1001	the fatty acid profile	980:1001	the fatty acid profile of A7	980:1007	However, the optimum growth of A7 was at alkaline pH (9.0) and 55°C, compared to pH 7.0 at 60°C for GGR1, and the fatty acid profile of A7 contained 1.7-times more C17:0 iso than GGR1.
29482868	6	24	theme	draft	1059:1063	arg1	sequence					1072:1079	The draft genome sequence	1055:1079	The draft genome sequence	1055:1079	The draft genome sequence revealed that H. saccincola A7 possessed a cellulosome-like extracellular macromolecular complex, which has also been found for Clostridium thermocellum and C. clariflavum.
29482868	7	25	theme	more	1353:1356	arg1	diversity					1358:1366	more glycoside hydrolases (GHs) belonging to GH families-11 and -2, and more diversity	1281:1366	more glycoside hydrolases (GHs) belonging to GH families-11 and -2, and more diversity of xylanolytic enzymes	1281:1389	H. saccincola A7 contained more glycoside hydrolases (GHs) belonging to GH families-11 and -2, and more diversity of xylanolytic enzymes, than C. thermocellum and C. clariflavum.
29482868	1	26	theme	anaerobic	171:179	arg1	bacterium					207:215	An anaerobic, cellulolytic-xylanolytic bacterium	168:215	An anaerobic, cellulolytic-xylanolytic bacterium	168:215	An anaerobic, cellulolytic-xylanolytic bacterium, designated strain A7, was isolated from a cellulose-degrading bacterial community inhabiting bovine manure compost on Ishigaki Island, Japan, by enrichment culture using unpretreated corn stover as the sole carbon source.
29482868	1	27	theme	unpretreated	388:399	arg1	source					432:437	the sole carbon source	416:437	the sole carbon source	416:437	An anaerobic, cellulolytic-xylanolytic bacterium, designated strain A7, was isolated from a cellulose-degrading bacterial community inhabiting bovine manure compost on Ishigaki Island, Japan, by enrichment culture using unpretreated corn stover as the sole carbon source.
29482868	1	27	theme	unpretreated	388:399	arg1	stover					406:411	unpretreated corn stover	388:411	unpretreated corn stover	388:411	An anaerobic, cellulolytic-xylanolytic bacterium, designated strain A7, was isolated from a cellulose-degrading bacterial community inhabiting bovine manure compost on Ishigaki Island, Japan, by enrichment culture using unpretreated corn stover as the sole carbon source.
29482868	6	28	contain	possessed	1112:1120	arg1	A7					1109:1110	A7	1109:1110	A7	1109:1110	The draft genome sequence revealed that H. saccincola A7 possessed a cellulosome-like extracellular macromolecular complex, which has also been found for Clostridium thermocellum and C. clariflavum.
29482868	6	28	contain	possessed	1112:1120	arg2	complex					1170:1176	a cellulosome-like extracellular macromolecular complex	1122:1176	a cellulosome-like extracellular macromolecular complex	1122:1176	The draft genome sequence revealed that H. saccincola A7 possessed a cellulosome-like extracellular macromolecular complex, which has also been found for Clostridium thermocellum and C. clariflavum.
29482868	5	29	theme	A7	901:902	arg1	growth					891:896	the optimum growth	879:896	the optimum growth of A7	879:902	However, the optimum growth of A7 was at alkaline pH (9.0) and 55°C, compared to pH 7.0 at 60°C for GGR1, and the fatty acid profile of A7 contained 1.7-times more C17:0 iso than GGR1.
29482868	8	30	theme	ribulose-phosphate	1594:1611	arg1	3-epimerase					1613:1623	ribulose-phosphate 3-epimerase	1594:1623	ribulose-phosphate 3-epimerase	1594:1623	H. saccincola A7 could grow on xylan because it encoded essential genes for xylose metabolism, such as a xylose transporter, xylose isomerase, xylulokinase, and ribulose-phosphate 3-epimerase, which are absent from C. thermocellum.
29482868	7	31	theme	xylanolytic	1371:1381	arg1	enzymes					1383:1389	xylanolytic enzymes	1371:1389	xylanolytic enzymes	1371:1389	H. saccincola A7 contained more glycoside hydrolases (GHs) belonging to GH families-11 and -2, and more diversity of xylanolytic enzymes, than C. thermocellum and C. clariflavum.
29482868	3	32	theme	DSM	744:746	arg1	101079T					748:754	= DSM 101079T	742:754	= DSM 101079T	742:754	Strain A7 was identified as Herbivorax saccincola by DNA-DNA hybridization, and phylogenetic analysis based on 16S rRNA gene sequences showed that it was closely related to H. saccincola GGR1 (= DSM 101079T).
29482868	3	32	theme	DSM	744:746	arg1	GGR1					736:739	H. saccincola GGR1	722:739	H. saccincola GGR1 (= DSM 101079T)	722:755	Strain A7 was identified as Herbivorax saccincola by DNA-DNA hybridization, and phylogenetic analysis based on 16S rRNA gene sequences showed that it was closely related to H. saccincola GGR1 (= DSM 101079T).
29482868	4	33	theme	phenotypic	818:827	arg1	characteristics					829:843	quite similar phenotypic characteristics	804:843	quite similar phenotypic characteristics	804:843	H. saccincola A7 (= JCM 31827=DSM 104321) had quite similar phenotypic characteristics to those of strain GGR1.
29482868	3	34	theme	Herbivorax	577:586	arg1	saccincola					588:597	Herbivorax saccincola	577:597	Herbivorax saccincola	577:597	Strain A7 was identified as Herbivorax saccincola by DNA-DNA hybridization, and phylogenetic analysis based on 16S rRNA gene sequences showed that it was closely related to H. saccincola GGR1 (= DSM 101079T).
29482868	3	34	theme	Herbivorax	577:586	arg1	A7					556:557	Strain A7	549:557	Strain A7	549:557	Strain A7 was identified as Herbivorax saccincola by DNA-DNA hybridization, and phylogenetic analysis based on 16S rRNA gene sequences showed that it was closely related to H. saccincola GGR1 (= DSM 101079T).
29482868	3	35	theme	16S	660:662	arg1	sequences					674:682	16S rRNA gene sequences	660:682	16S rRNA gene sequences	660:682	Strain A7 was identified as Herbivorax saccincola by DNA-DNA hybridization, and phylogenetic analysis based on 16S rRNA gene sequences showed that it was closely related to H. saccincola GGR1 (= DSM 101079T).
29482868	0	36	theme	draft	34:38	arg1	sequence					47:54	high-quality draft genome sequence	21:54	high-quality draft genome sequence	21:54	Characterization and high-quality draft genome sequence of Herbivorax saccincola A7, an anaerobic, alkaliphilic, thermophilic, cellulolytic, and xylanolytic bacterium.
29482868	7	37	theme	GH	1326:1327	arg1	families-11					1329:1339	GH families-11	1326:1339	GH families-11	1326:1339	H. saccincola A7 contained more glycoside hydrolases (GHs) belonging to GH families-11 and -2, and more diversity of xylanolytic enzymes, than C. thermocellum and C. clariflavum.
29482868	6	38	theme	extracellular	1141:1153	arg1	complex					1170:1176	a cellulosome-like extracellular macromolecular complex	1122:1176	a cellulosome-like extracellular macromolecular complex	1122:1176	The draft genome sequence revealed that H. saccincola A7 possessed a cellulosome-like extracellular macromolecular complex, which has also been found for Clostridium thermocellum and C. clariflavum.
29482868	0	39	theme	high-quality	21:32	arg1	sequence					47:54	high-quality draft genome sequence	21:54	high-quality draft genome sequence	21:54	Characterization and high-quality draft genome sequence of Herbivorax saccincola A7, an anaerobic, alkaliphilic, thermophilic, cellulolytic, and xylanolytic bacterium.
29482868	1	40	theme	bovine	311:316	arg1	compost					325:331	bovine manure compost	311:331	bovine manure compost on Ishigaki Island	311:350	An anaerobic, cellulolytic-xylanolytic bacterium, designated strain A7, was isolated from a cellulose-degrading bacterial community inhabiting bovine manure compost on Ishigaki Island, Japan, by enrichment culture using unpretreated corn stover as the sole carbon source.
29482868	0	41	theme	thermophilic	113:124	arg1	bacterium					157:165	an anaerobic, alkaliphilic, thermophilic, cellulolytic, and xylanolytic bacterium	85:165	an anaerobic, alkaliphilic, thermophilic, cellulolytic, and xylanolytic bacterium	85:165	Characterization and high-quality draft genome sequence of Herbivorax saccincola A7, an anaerobic, alkaliphilic, thermophilic, cellulolytic, and xylanolytic bacterium.
29482868	0	41	theme	thermophilic	113:124	arg1	A7					81:82	A7	81:82	A7	81:82	Characterization and high-quality draft genome sequence of Herbivorax saccincola A7, an anaerobic, alkaliphilic, thermophilic, cellulolytic, and xylanolytic bacterium.
29482868	1	42	theme	corn	401:404	arg1	source					432:437	the sole carbon source	416:437	the sole carbon source	416:437	An anaerobic, cellulolytic-xylanolytic bacterium, designated strain A7, was isolated from a cellulose-degrading bacterial community inhabiting bovine manure compost on Ishigaki Island, Japan, by enrichment culture using unpretreated corn stover as the sole carbon source.
29482868	1	42	theme	corn	401:404	arg1	stover					406:411	unpretreated corn stover	388:411	unpretreated corn stover	388:411	An anaerobic, cellulolytic-xylanolytic bacterium, designated strain A7, was isolated from a cellulose-degrading bacterial community inhabiting bovine manure compost on Ishigaki Island, Japan, by enrichment culture using unpretreated corn stover as the sole carbon source.
29482868	5	43	theme	alkaline	911:918	arg1	pH					920:921	alkaline pH (9.0)	911:927	alkaline pH (9.0)	911:927	However, the optimum growth of A7 was at alkaline pH (9.0) and 55°C, compared to pH 7.0 at 60°C for GGR1, and the fatty acid profile of A7 contained 1.7-times more C17:0 iso than GGR1.
29482868	9	44	theme	great	1715:1719	arg1	potential					1721:1729	great potential	1715:1729	great potential	1715:1729	These results indicated that H. saccincola A7 has great potential as a microorganism that can effectively degrade lignocellulosic biomass.
29482868	9	44	theme	great	1715:1719	arg1	microorganism					1736:1748	a microorganism	1734:1748	a microorganism that can effectively degrade lignocellulosic biomass	1734:1801	These results indicated that H. saccincola A7 has great potential as a microorganism that can effectively degrade lignocellulosic biomass.
29482868	1	45	theme	manure	318:323	arg1	compost					325:331	bovine manure compost	311:331	bovine manure compost on Ishigaki Island	311:350	An anaerobic, cellulolytic-xylanolytic bacterium, designated strain A7, was isolated from a cellulose-degrading bacterial community inhabiting bovine manure compost on Ishigaki Island, Japan, by enrichment culture using unpretreated corn stover as the sole carbon source.
29482868	9	46	theme	lignocellulosic	1779:1793	arg1	biomass					1795:1801	lignocellulosic biomass	1779:1801	lignocellulosic biomass	1779:1801	These results indicated that H. saccincola A7 has great potential as a microorganism that can effectively degrade lignocellulosic biomass.
29482868	3	47	theme	rRNA	664:667	arg1	sequences					674:682	16S rRNA gene sequences	660:682	16S rRNA gene sequences	660:682	Strain A7 was identified as Herbivorax saccincola by DNA-DNA hybridization, and phylogenetic analysis based on 16S rRNA gene sequences showed that it was closely related to H. saccincola GGR1 (= DSM 101079T).
29482868	3	48	theme	DNA-DNA	602:608	arg1	hybridization					610:622	DNA-DNA hybridization	602:622	DNA-DNA hybridization	602:622	Strain A7 was identified as Herbivorax saccincola by DNA-DNA hybridization, and phylogenetic analysis based on 16S rRNA gene sequences showed that it was closely related to H. saccincola GGR1 (= DSM 101079T).
29482868	9	49	contain	has	1711:1713	arg1	A7					1708:1709	A7	1708:1709	A7	1708:1709	These results indicated that H. saccincola A7 has great potential as a microorganism that can effectively degrade lignocellulosic biomass.
29482868	9	49	contain	has	1711:1713	arg2	microorganism					1736:1748	a microorganism	1734:1748	a microorganism that can effectively degrade lignocellulosic biomass	1734:1801	These results indicated that H. saccincola A7 has great potential as a microorganism that can effectively degrade lignocellulosic biomass.
29482868	9	49	contain	has	1711:1713	arg2	potential					1721:1729	great potential	1715:1729	great potential	1715:1729	These results indicated that H. saccincola A7 has great potential as a microorganism that can effectively degrade lignocellulosic biomass.
29482868	0	50	theme	genome	40:45	arg1	sequence					47:54	high-quality draft genome sequence	21:54	high-quality draft genome sequence	21:54	Characterization and high-quality draft genome sequence of Herbivorax saccincola A7, an anaerobic, alkaliphilic, thermophilic, cellulolytic, and xylanolytic bacterium.
29482868	0	51	theme	cellulolytic	127:138	arg1	bacterium					157:165	an anaerobic, alkaliphilic, thermophilic, cellulolytic, and xylanolytic bacterium	85:165	an anaerobic, alkaliphilic, thermophilic, cellulolytic, and xylanolytic bacterium	85:165	Characterization and high-quality draft genome sequence of Herbivorax saccincola A7, an anaerobic, alkaliphilic, thermophilic, cellulolytic, and xylanolytic bacterium.
29482868	0	51	theme	cellulolytic	127:138	arg1	A7					81:82	A7	81:82	A7	81:82	Characterization and high-quality draft genome sequence of Herbivorax saccincola A7, an anaerobic, alkaliphilic, thermophilic, cellulolytic, and xylanolytic bacterium.
29482868	5	52	theme	fatty	984:988	arg1	profile					995:1001	the fatty acid profile	980:1001	the fatty acid profile of A7	980:1007	However, the optimum growth of A7 was at alkaline pH (9.0) and 55°C, compared to pH 7.0 at 60°C for GGR1, and the fatty acid profile of A7 contained 1.7-times more C17:0 iso than GGR1.
29482868	4	53	theme	31827=DSM	782:790	arg1	104321					792:797	= JCM 31827=DSM 104321	776:797	= JCM 31827=DSM 104321	776:797	H. saccincola A7 (= JCM 31827=DSM 104321) had quite similar phenotypic characteristics to those of strain GGR1.
29482868	4	53	theme	31827=DSM	782:790	arg1	A7					772:773	A7	772:773	A7	772:773	H. saccincola A7 (= JCM 31827=DSM 104321) had quite similar phenotypic characteristics to those of strain GGR1.
29482868	1	54	theme	strain	229:234	arg1	A7					236:237	strain A7	229:237	strain A7	229:237	An anaerobic, cellulolytic-xylanolytic bacterium, designated strain A7, was isolated from a cellulose-degrading bacterial community inhabiting bovine manure compost on Ishigaki Island, Japan, by enrichment culture using unpretreated corn stover as the sole carbon source.
29482868	5	55	contain	contained	1009:1017	arg1	profile					995:1001	the fatty acid profile	980:1001	the fatty acid profile of A7	980:1007	However, the optimum growth of A7 was at alkaline pH (9.0) and 55°C, compared to pH 7.0 at 60°C for GGR1, and the fatty acid profile of A7 contained 1.7-times more C17:0 iso than GGR1.
29482868	5	55	contain	contained	1009:1017	arg2	1.7-times					1019:1027	1.7-times	1019:1027	1.7-times more C17:0 iso than GGR1	1019:1052	However, the optimum growth of A7 was at alkaline pH (9.0) and 55°C, compared to pH 7.0 at 60°C for GGR1, and the fatty acid profile of A7 contained 1.7-times more C17:0 iso than GGR1.
29482868	0	56	theme	xylanolytic	145:155	arg1	bacterium					157:165	an anaerobic, alkaliphilic, thermophilic, cellulolytic, and xylanolytic bacterium	85:165	an anaerobic, alkaliphilic, thermophilic, cellulolytic, and xylanolytic bacterium	85:165	Characterization and high-quality draft genome sequence of Herbivorax saccincola A7, an anaerobic, alkaliphilic, thermophilic, cellulolytic, and xylanolytic bacterium.
29482868	0	56	theme	xylanolytic	145:155	arg1	A7					81:82	A7	81:82	A7	81:82	Characterization and high-quality draft genome sequence of Herbivorax saccincola A7, an anaerobic, alkaliphilic, thermophilic, cellulolytic, and xylanolytic bacterium.
29482868	5	57	theme	acid	990:993	arg1	profile					995:1001	the fatty acid profile	980:1001	the fatty acid profile of A7	980:1007	However, the optimum growth of A7 was at alkaline pH (9.0) and 55°C, compared to pH 7.0 at 60°C for GGR1, and the fatty acid profile of A7 contained 1.7-times more C17:0 iso than GGR1.
29482868	1	58	attach	isolated	244:251	arg1	community					290:298	a cellulose-degrading bacterial community	258:298	a cellulose-degrading bacterial community inhabiting bovine manure compost on Ishigaki Island	258:350	An anaerobic, cellulolytic-xylanolytic bacterium, designated strain A7, was isolated from a cellulose-degrading bacterial community inhabiting bovine manure compost on Ishigaki Island, Japan, by enrichment culture using unpretreated corn stover as the sole carbon source.
29482868	1	58	attach	isolated	244:251	arg2	bacterium					207:215	An anaerobic, cellulolytic-xylanolytic bacterium	168:215	An anaerobic, cellulolytic-xylanolytic bacterium	168:215	An anaerobic, cellulolytic-xylanolytic bacterium, designated strain A7, was isolated from a cellulose-degrading bacterial community inhabiting bovine manure compost on Ishigaki Island, Japan, by enrichment culture using unpretreated corn stover as the sole carbon source.
29482868	1	58	attach	isolated	244:251	arg1	Japan					353:357	Japan	353:357	Japan	353:357	An anaerobic, cellulolytic-xylanolytic bacterium, designated strain A7, was isolated from a cellulose-degrading bacterial community inhabiting bovine manure compost on Ishigaki Island, Japan, by enrichment culture using unpretreated corn stover as the sole carbon source.
29482868	1	59	theme	Ishigaki	336:343	arg1	Island					345:350	Ishigaki Island	336:350	Ishigaki Island	336:350	An anaerobic, cellulolytic-xylanolytic bacterium, designated strain A7, was isolated from a cellulose-degrading bacterial community inhabiting bovine manure compost on Ishigaki Island, Japan, by enrichment culture using unpretreated corn stover as the sole carbon source.
29482868	1	60	theme	sole	420:423	arg1	source					432:437	the sole carbon source	416:437	the sole carbon source	416:437	An anaerobic, cellulolytic-xylanolytic bacterium, designated strain A7, was isolated from a cellulose-degrading bacterial community inhabiting bovine manure compost on Ishigaki Island, Japan, by enrichment culture using unpretreated corn stover as the sole carbon source.
29482868	1	60	theme	sole	420:423	arg1	stover					406:411	unpretreated corn stover	388:411	unpretreated corn stover	388:411	An anaerobic, cellulolytic-xylanolytic bacterium, designated strain A7, was isolated from a cellulose-degrading bacterial community inhabiting bovine manure compost on Ishigaki Island, Japan, by enrichment culture using unpretreated corn stover as the sole carbon source.
29482868	1	61	dep	anaerobic	171:179	arg1	cellulolytic-xylanolytic					182:205	cellulolytic-xylanolytic	182:205	cellulolytic-xylanolytic	182:205	An anaerobic, cellulolytic-xylanolytic bacterium, designated strain A7, was isolated from a cellulose-degrading bacterial community inhabiting bovine manure compost on Ishigaki Island, Japan, by enrichment culture using unpretreated corn stover as the sole carbon source.
29482868	4	62	theme	strain	857:862	arg1	GGR1					864:867	strain GGR1	857:867	strain GGR1	857:867	H. saccincola A7 (= JCM 31827=DSM 104321) had quite similar phenotypic characteristics to those of strain GGR1.
29482868	8	63	theme	xylose	1538:1543	arg1	transporter					1545:1555	a xylose transporter	1536:1555	a xylose transporter	1536:1555	H. saccincola A7 could grow on xylan because it encoded essential genes for xylose metabolism, such as a xylose transporter, xylose isomerase, xylulokinase, and ribulose-phosphate 3-epimerase, which are absent from C. thermocellum.
29482868	8	63	theme	xylose	1538:1543	arg1	absent					1636:1641	absent	1636:1641	absent	1636:1641	H. saccincola A7 could grow on xylan because it encoded essential genes for xylose metabolism, such as a xylose transporter, xylose isomerase, xylulokinase, and ribulose-phosphate 3-epimerase, which are absent from C. thermocellum.
29482868	1	64	theme	carbon	425:430	arg1	source					432:437	the sole carbon source	416:437	the sole carbon source	416:437	An anaerobic, cellulolytic-xylanolytic bacterium, designated strain A7, was isolated from a cellulose-degrading bacterial community inhabiting bovine manure compost on Ishigaki Island, Japan, by enrichment culture using unpretreated corn stover as the sole carbon source.
29482868	1	64	theme	carbon	425:430	arg1	stover					406:411	unpretreated corn stover	388:411	unpretreated corn stover	388:411	An anaerobic, cellulolytic-xylanolytic bacterium, designated strain A7, was isolated from a cellulose-degrading bacterial community inhabiting bovine manure compost on Ishigaki Island, Japan, by enrichment culture using unpretreated corn stover as the sole carbon source.
29482868	3	65	theme	phylogenetic	629:640	arg1	analysis					642:649	phylogenetic analysis	629:649	phylogenetic analysis based on 16S rRNA gene sequences	629:682	Strain A7 was identified as Herbivorax saccincola by DNA-DNA hybridization, and phylogenetic analysis based on 16S rRNA gene sequences showed that it was closely related to H. saccincola GGR1 (= DSM 101079T).
29482868	6	66	theme	cellulosome-like	1124:1139	arg1	complex					1170:1176	a cellulosome-like extracellular macromolecular complex	1122:1176	a cellulosome-like extracellular macromolecular complex	1122:1176	The draft genome sequence revealed that H. saccincola A7 possessed a cellulosome-like extracellular macromolecular complex, which has also been found for Clostridium thermocellum and C. clariflavum.
29482868	5	67	dep	pH	920:921	arg1	compared					939:946	compared	939:946	compared to pH 7.0 at 60°C for GGR1	939:973	However, the optimum growth of A7 was at alkaline pH (9.0) and 55°C, compared to pH 7.0 at 60°C for GGR1, and the fatty acid profile of A7 contained 1.7-times more C17:0 iso than GGR1.
29482868	3	68	theme	gene	669:672	arg1	sequences					674:682	16S rRNA gene sequences	660:682	16S rRNA gene sequences	660:682	Strain A7 was identified as Herbivorax saccincola by DNA-DNA hybridization, and phylogenetic analysis based on 16S rRNA gene sequences showed that it was closely related to H. saccincola GGR1 (= DSM 101079T).
29482868	7	69	theme	more	1281:1284	arg1	diversity					1358:1366	more glycoside hydrolases (GHs) belonging to GH families-11 and -2, and more diversity	1281:1366	more glycoside hydrolases (GHs) belonging to GH families-11 and -2, and more diversity of xylanolytic enzymes	1281:1389	H. saccincola A7 contained more glycoside hydrolases (GHs) belonging to GH families-11 and -2, and more diversity of xylanolytic enzymes, than C. thermocellum and C. clariflavum.
29482868	4	70	contain	had	800:802	arg1	104321					792:797	= JCM 31827=DSM 104321	776:797	= JCM 31827=DSM 104321	776:797	H. saccincola A7 (= JCM 31827=DSM 104321) had quite similar phenotypic characteristics to those of strain GGR1.
29482868	4	70	contain	had	800:802	arg1	A7					772:773	A7	772:773	A7	772:773	H. saccincola A7 (= JCM 31827=DSM 104321) had quite similar phenotypic characteristics to those of strain GGR1.
29482868	4	70	contain	had	800:802	arg2	characteristics					829:843	quite similar phenotypic characteristics	804:843	quite similar phenotypic characteristics	804:843	H. saccincola A7 (= JCM 31827=DSM 104321) had quite similar phenotypic characteristics to those of strain GGR1.
29482868	8	71	theme	essential	1489:1497	arg1	transporter					1545:1555	a xylose transporter	1536:1555	a xylose transporter	1536:1555	H. saccincola A7 could grow on xylan because it encoded essential genes for xylose metabolism, such as a xylose transporter, xylose isomerase, xylulokinase, and ribulose-phosphate 3-epimerase, which are absent from C. thermocellum.
29482868	8	71	theme	essential	1489:1497	arg1	it					1478:1479	it	1478:1479	it	1478:1479	H. saccincola A7 could grow on xylan because it encoded essential genes for xylose metabolism, such as a xylose transporter, xylose isomerase, xylulokinase, and ribulose-phosphate 3-epimerase, which are absent from C. thermocellum.
29482868	8	71	theme	essential	1489:1497	arg1	3-epimerase					1613:1623	ribulose-phosphate 3-epimerase	1594:1623	ribulose-phosphate 3-epimerase	1594:1623	H. saccincola A7 could grow on xylan because it encoded essential genes for xylose metabolism, such as a xylose transporter, xylose isomerase, xylulokinase, and ribulose-phosphate 3-epimerase, which are absent from C. thermocellum.
29482868	8	71	theme	essential	1489:1497	arg1	isomerase					1565:1573	xylose isomerase	1558:1573	xylose isomerase	1558:1573	H. saccincola A7 could grow on xylan because it encoded essential genes for xylose metabolism, such as a xylose transporter, xylose isomerase, xylulokinase, and ribulose-phosphate 3-epimerase, which are absent from C. thermocellum.
29482868	8	71	theme	essential	1489:1497	arg1	genes					1499:1503	essential genes	1489:1503	essential genes	1489:1503	H. saccincola A7 could grow on xylan because it encoded essential genes for xylose metabolism, such as a xylose transporter, xylose isomerase, xylulokinase, and ribulose-phosphate 3-epimerase, which are absent from C. thermocellum.
29482868	8	71	theme	essential	1489:1497	arg1	xylulokinase					1576:1587	xylulokinase	1576:1587	xylulokinase	1576:1587	H. saccincola A7 could grow on xylan because it encoded essential genes for xylose metabolism, such as a xylose transporter, xylose isomerase, xylulokinase, and ribulose-phosphate 3-epimerase, which are absent from C. thermocellum.
29482868	0	72	theme	A7	81:82	arg1	sequence					47:54	high-quality draft genome sequence	21:54	high-quality draft genome sequence	21:54	Characterization and high-quality draft genome sequence of Herbivorax saccincola A7, an anaerobic, alkaliphilic, thermophilic, cellulolytic, and xylanolytic bacterium.
29482868	0	72	theme	A7	81:82	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization and high-quality draft genome sequence of Herbivorax saccincola A7, an anaerobic, alkaliphilic, thermophilic, cellulolytic, and xylanolytic bacterium.
29482868	7	73	dep	more	1281:1284	arg1	hydrolases					1296:1305	glycoside hydrolases	1286:1305	glycoside hydrolases (GHs) belonging to GH families-11 and -2	1286:1346	H. saccincola A7 contained more glycoside hydrolases (GHs) belonging to GH families-11 and -2, and more diversity of xylanolytic enzymes, than C. thermocellum and C. clariflavum.
29482868	7	73	dep	more	1281:1284	arg1	GHs					1308:1310	GHs	1308:1310	GHs	1308:1310	H. saccincola A7 contained more glycoside hydrolases (GHs) belonging to GH families-11 and -2, and more diversity of xylanolytic enzymes, than C. thermocellum and C. clariflavum.
29482868	4	74	theme	JCM	778:780	arg1	104321					792:797	= JCM 31827=DSM 104321	776:797	= JCM 31827=DSM 104321	776:797	H. saccincola A7 (= JCM 31827=DSM 104321) had quite similar phenotypic characteristics to those of strain GGR1.
29482868	4	74	theme	JCM	778:780	arg1	A7					772:773	A7	772:773	A7	772:773	H. saccincola A7 (= JCM 31827=DSM 104321) had quite similar phenotypic characteristics to those of strain GGR1.
29482868	5	75	dep	more	1029:1032	arg1	iso					1040:1042	C17:0 iso	1034:1042	C17:0 iso	1034:1042	However, the optimum growth of A7 was at alkaline pH (9.0) and 55°C, compared to pH 7.0 at 60°C for GGR1, and the fatty acid profile of A7 contained 1.7-times more C17:0 iso than GGR1.
29482868	8	76	theme	xylose	1509:1514	arg1	metabolism					1516:1525	xylose metabolism	1509:1525	xylose metabolism	1509:1525	H. saccincola A7 could grow on xylan because it encoded essential genes for xylose metabolism, such as a xylose transporter, xylose isomerase, xylulokinase, and ribulose-phosphate 3-epimerase, which are absent from C. thermocellum.
29482868	4	77	theme	=	776:776	arg1	104321					792:797	= JCM 31827=DSM 104321	776:797	= JCM 31827=DSM 104321	776:797	H. saccincola A7 (= JCM 31827=DSM 104321) had quite similar phenotypic characteristics to those of strain GGR1.
29482868	4	77	theme	=	776:776	arg1	A7					772:773	A7	772:773	A7	772:773	H. saccincola A7 (= JCM 31827=DSM 104321) had quite similar phenotypic characteristics to those of strain GGR1.
29482868	7	78	contain	contained	1271:1279	arg1	A7					1268:1269	A7	1268:1269	A7	1268:1269	H. saccincola A7 contained more glycoside hydrolases (GHs) belonging to GH families-11 and -2, and more diversity of xylanolytic enzymes, than C. thermocellum and C. clariflavum.
29482868	7	78	contain	contained	1271:1279	arg2	diversity					1358:1366	more glycoside hydrolases (GHs) belonging to GH families-11 and -2, and more diversity	1281:1366	more glycoside hydrolases (GHs) belonging to GH families-11 and -2, and more diversity of xylanolytic enzymes	1281:1389	H. saccincola A7 contained more glycoside hydrolases (GHs) belonging to GH families-11 and -2, and more diversity of xylanolytic enzymes, than C. thermocellum and C. clariflavum.
29870157	2	0	theme	resistance	353:362	arg1	exercise					364:371	resistance exercise	353:371	resistance exercise after an endurance exercise	353:399	We aimed to evaluate the effect of a preresistance exercise carbohydrate meal versus fat meal on plasma cytokine responses to resistance exercise after an endurance exercise earlier that day.
29870157	12	1	theme	circulating	1879:1889	arg1	levels					1903:1908	postresistance exercise circulating plasma IL-6 levels	1855:1908	postresistance exercise circulating plasma IL-6 levels	1855:1908	The composition of the pre-exercise meal did in general not influence cytokine responses in the postresistance exercise period, except postresistance exercise circulating plasma IL-6 levels being higher in the fat condition compared with carbohydrate.
29870157	6	2	theme	fatty	1101:1105	arg1	acids					1107:1111	free fatty acids	1096:1111	free fatty acids	1096:1111	Plasma glucose and insulin were higher in the postprandial period before the start of the resistance exercise on the carbohydrate condition, while free fatty acids were reduced.
29870157	4	3	theme	110 g	608:612	arg1	fat					646:648	9 g fat	642:648	9 g fat	642:648	Either a carbohydrate (110 g carbohydrate, 52 g protein, 9 g fat; ~750 kcal) or an isocaloric fat meal (20 gr carbohydrate, 52 g protein, 51 g fat) was provided 2 h before resistance exercise.
29870157	4	3	theme	110 g	608:612	arg1	carbohydrate					614:625	110 g carbohydrate	608:625	110 g carbohydrate	608:625	Either a carbohydrate (110 g carbohydrate, 52 g protein, 9 g fat; ~750 kcal) or an isocaloric fat meal (20 gr carbohydrate, 52 g protein, 51 g fat) was provided 2 h before resistance exercise.
29870157	4	3	theme	110 g	608:612	arg1	protein					633:639	52 g protein	628:639	52 g protein	628:639	Either a carbohydrate (110 g carbohydrate, 52 g protein, 9 g fat; ~750 kcal) or an isocaloric fat meal (20 gr carbohydrate, 52 g protein, 51 g fat) was provided 2 h before resistance exercise.
29870157	2	4	theme	carbohydrate	287:298	arg1	meal					300:303	carbohydrate meal	287:303	carbohydrate meal	287:303	We aimed to evaluate the effect of a preresistance exercise carbohydrate meal versus fat meal on plasma cytokine responses to resistance exercise after an endurance exercise earlier that day.
29870157	12	5	theme	IL-6	1898:1901	arg1	levels					1903:1908	postresistance exercise circulating plasma IL-6 levels	1855:1908	postresistance exercise circulating plasma IL-6 levels	1855:1908	The composition of the pre-exercise meal did in general not influence cytokine responses in the postresistance exercise period, except postresistance exercise circulating plasma IL-6 levels being higher in the fat condition compared with carbohydrate.
29870157	3	6	with	days	499:502	arg1	exercise					519:526	endurance exercise	509:526	endurance exercise	509:526	Thirteen young, healthy, recreationally active males performed two experimental days with endurance exercise in the morning and resistance exercise in the afternoon.
29870157	2	7	theme	preresistance	264:276	arg1	exercise					278:285	a preresistance exercise carbohydrate meal versus fat meal	262:319	exercise	278:285	We aimed to evaluate the effect of a preresistance exercise carbohydrate meal versus fat meal on plasma cytokine responses to resistance exercise after an endurance exercise earlier that day.
29870157	2	8	theme	meal	316:319	arg1	effect					252:257	the effect	248:257	the effect of a preresistance exercise carbohydrate meal versus fat meal on plasma cytokine responses to resistance exercise after an endurance exercise	248:399	We aimed to evaluate the effect of a preresistance exercise carbohydrate meal versus fat meal on plasma cytokine responses to resistance exercise after an endurance exercise earlier that day.
29870157	12	9	theme	postresistance	1855:1868	arg1	levels					1903:1908	postresistance exercise circulating plasma IL-6 levels	1855:1908	postresistance exercise circulating plasma IL-6 levels	1855:1908	The composition of the pre-exercise meal did in general not influence cytokine responses in the postresistance exercise period, except postresistance exercise circulating plasma IL-6 levels being higher in the fat condition compared with carbohydrate.
29870157	4	10	theme	9 g	642:644	arg1	fat					646:648	9 g fat	642:648	9 g fat	642:648	Either a carbohydrate (110 g carbohydrate, 52 g protein, 9 g fat; ~750 kcal) or an isocaloric fat meal (20 gr carbohydrate, 52 g protein, 51 g fat) was provided 2 h before resistance exercise.
29870157	4	10	theme	9 g	642:644	arg1	carbohydrate					614:625	110 g carbohydrate	608:625	110 g carbohydrate	608:625	Either a carbohydrate (110 g carbohydrate, 52 g protein, 9 g fat; ~750 kcal) or an isocaloric fat meal (20 gr carbohydrate, 52 g protein, 51 g fat) was provided 2 h before resistance exercise.
29870157	0	11	theme	trained	118:124	arg1	males					134:138	trained healthy males	118:138	trained healthy males	118:138	Plasma cytokine responses to resistance exercise with different nutrient availability on a concurrent exercise day in trained healthy males.
29870157	1	12	theme	cytokine	207:214	arg1	responses					216:224	cytokine responses	207:224	cytokine responses	207:224	Carbohydrate availability is proposed as a potential regulator of cytokine responses.
29870157	4	13	dep	carbohydrate	594:605	arg1	fat					646:648	9 g fat	642:648	9 g fat	642:648	Either a carbohydrate (110 g carbohydrate, 52 g protein, 9 g fat; ~750 kcal) or an isocaloric fat meal (20 gr carbohydrate, 52 g protein, 51 g fat) was provided 2 h before resistance exercise.
29870157	4	13	dep	carbohydrate	594:605	arg1	carbohydrate					614:625	110 g carbohydrate	608:625	110 g carbohydrate	608:625	Either a carbohydrate (110 g carbohydrate, 52 g protein, 9 g fat; ~750 kcal) or an isocaloric fat meal (20 gr carbohydrate, 52 g protein, 51 g fat) was provided 2 h before resistance exercise.
29870157	4	13	dep	carbohydrate	594:605	arg1	protein					633:639	52 g protein	628:639	52 g protein	628:639	Either a carbohydrate (110 g carbohydrate, 52 g protein, 9 g fat; ~750 kcal) or an isocaloric fat meal (20 gr carbohydrate, 52 g protein, 51 g fat) was provided 2 h before resistance exercise.
29870157	5	14	theme	regular	813:819	arg1	intervals					826:834	regular time intervals	813:834	regular time intervals	813:834	Blood was taken at baseline and at regular time intervals to measure circulating plasma cytokine levels (e.g. IL-6, IL-8, IL-10, IL-15, TNFα, ANGPTL4, decorin and MCP-1).
29870157	12	15	theme	exercise	1831:1838	arg1	period					1840:1845	the postresistance exercise period	1812:1845	the postresistance exercise period	1812:1845	The composition of the pre-exercise meal did in general not influence cytokine responses in the postresistance exercise period, except postresistance exercise circulating plasma IL-6 levels being higher in the fat condition compared with carbohydrate.
29870157	0	16	from	day	111:113	arg1	males					134:138	trained healthy males	118:138	trained healthy males	118:138	Plasma cytokine responses to resistance exercise with different nutrient availability on a concurrent exercise day in trained healthy males.
29870157	4	17	theme	52 g	709:712	arg1	carbohydrate					695:706	20 gr carbohydrate	689:706	20 gr carbohydrate	689:706	Either a carbohydrate (110 g carbohydrate, 52 g protein, 9 g fat; ~750 kcal) or an isocaloric fat meal (20 gr carbohydrate, 52 g protein, 51 g fat) was provided 2 h before resistance exercise.
29870157	4	17	theme	52 g	709:712	arg1	protein					714:720	52 g protein	709:720	52 g protein	709:720	Either a carbohydrate (110 g carbohydrate, 52 g protein, 9 g fat; ~750 kcal) or an isocaloric fat meal (20 gr carbohydrate, 52 g protein, 51 g fat) was provided 2 h before resistance exercise.
29870157	4	18	theme	fat	679:681	arg1	meal					683:686	an isocaloric fat meal	665:686	an isocaloric fat meal (20 gr carbohydrate, 52 g protein, 51 g fat)	665:731	Either a carbohydrate (110 g carbohydrate, 52 g protein, 9 g fat; ~750 kcal) or an isocaloric fat meal (20 gr carbohydrate, 52 g protein, 51 g fat) was provided 2 h before resistance exercise.
29870157	0	19	theme	nutrient	64:71	arg1	availability					73:84	different nutrient availability	54:84	different nutrient availability	54:84	Plasma cytokine responses to resistance exercise with different nutrient availability on a concurrent exercise day in trained healthy males.
29870157	5	20	theme	plasma	859:864	arg1	levels					875:880	circulating plasma cytokine levels	847:880	circulating plasma cytokine levels (e.g. IL-6, IL-8, IL-10, IL-15, TNFα, ANGPTL4, decorin and MCP-1)	847:946	Blood was taken at baseline and at regular time intervals to measure circulating plasma cytokine levels (e.g. IL-6, IL-8, IL-10, IL-15, TNFα, ANGPTL4, decorin and MCP-1).
29870157	7	21	theme	2 h	1130:1132	arg1	exercise					1149:1156	2 h postresistance exercise	1130:1156	2 h postresistance exercise	1130:1156	At 2 h postresistance exercise, IL-6 concentrations were higher in the fat condition compared to the carbohydrate condition (P < 0.05).
29870157	13	22	from	responses	2084:2092	arg1	humans					2129:2134	humans	2129:2134	humans	2129:2134	Our findings support the view that pre-exercise carbohydrate availability does not have a major impact on acute responses of circulating plasma cytokines in humans.
29870157	5	23	dep	IL-6	888:891	arg1	e.g.					883:886	e.g.	883:886	e.g.	883:886	Blood was taken at baseline and at regular time intervals to measure circulating plasma cytokine levels (e.g. IL-6, IL-8, IL-10, IL-15, TNFα, ANGPTL4, decorin and MCP-1).
29870157	0	24	theme	concurrent	91:100	arg1	day					111:113	a concurrent exercise day	89:113	a concurrent exercise day in trained healthy males	89:138	Plasma cytokine responses to resistance exercise with different nutrient availability on a concurrent exercise day in trained healthy males.
29870157	13	25	theme	carbohydrate	2020:2031	arg1	availability					2033:2044	pre-exercise carbohydrate availability	2007:2044	pre-exercise carbohydrate availability	2007:2044	Our findings support the view that pre-exercise carbohydrate availability does not have a major impact on acute responses of circulating plasma cytokines in humans.
29870157	12	26	theme	cytokine	1790:1797	arg1	responses					1799:1807	cytokine responses	1790:1807	cytokine responses	1790:1807	The composition of the pre-exercise meal did in general not influence cytokine responses in the postresistance exercise period, except postresistance exercise circulating plasma IL-6 levels being higher in the fat condition compared with carbohydrate.
29870157	4	27	dep	meal	683:686	arg1	carbohydrate					695:706	20 gr carbohydrate	689:706	20 gr carbohydrate	689:706	Either a carbohydrate (110 g carbohydrate, 52 g protein, 9 g fat; ~750 kcal) or an isocaloric fat meal (20 gr carbohydrate, 52 g protein, 51 g fat) was provided 2 h before resistance exercise.
29870157	4	27	dep	meal	683:686	arg1	protein					714:720	52 g protein	709:720	52 g protein	709:720	Either a carbohydrate (110 g carbohydrate, 52 g protein, 9 g fat; ~750 kcal) or an isocaloric fat meal (20 gr carbohydrate, 52 g protein, 51 g fat) was provided 2 h before resistance exercise.
29870157	4	27	dep	meal	683:686	arg1	fat					728:730	51 g fat	723:730	51 g fat	723:730	Either a carbohydrate (110 g carbohydrate, 52 g protein, 9 g fat; ~750 kcal) or an isocaloric fat meal (20 gr carbohydrate, 52 g protein, 51 g fat) was provided 2 h before resistance exercise.
29870157	12	28	theme	meal	1756:1759	arg1	composition					1724:1734	The composition	1720:1734	The composition of the pre-exercise meal	1720:1759	The composition of the pre-exercise meal did in general not influence cytokine responses in the postresistance exercise period, except postresistance exercise circulating plasma IL-6 levels being higher in the fat condition compared with carbohydrate.
29870157	10	29	theme	TNFα	1530:1533	arg1	levels					1535:1540	TNFα levels	1530:1540	TNFα levels	1530:1540	There were no differences between conditions on TNFα levels and levels remain constant when compared with baseline (P > 0.05).
29870157	0	30	theme	Plasma	0:5	arg1	responses					16:24	Plasma cytokine responses	0:24	Plasma cytokine responses to resistance exercise with different nutrient availability on a concurrent exercise day in trained healthy males.	0:139	Plasma cytokine responses to resistance exercise with different nutrient availability on a concurrent exercise day in trained healthy males.
29870157	13	31	theme	cytokines	2116:2124	arg1	responses					2084:2092	acute responses	2078:2092	acute responses of circulating plasma cytokines in humans	2078:2134	Our findings support the view that pre-exercise carbohydrate availability does not have a major impact on acute responses of circulating plasma cytokines in humans.
29870157	2	32	from	effect	252:257	arg1	responses					340:348	plasma cytokine responses	324:348	plasma cytokine responses to resistance exercise after an endurance exercise	324:399	We aimed to evaluate the effect of a preresistance exercise carbohydrate meal versus fat meal on plasma cytokine responses to resistance exercise after an endurance exercise earlier that day.
29870157	9	33	dep	plasma	1400:1405	arg1	IL-8					1407:1410	IL-8	1407:1410	IL-8	1407:1410	The pattern of increase in plasma IL-8 and IL-10 did not differ significantly between conditions (P > 0.05).
29870157	9	33	dep	plasma	1400:1405	arg1	IL-10					1416:1420	IL-10	1416:1420	IL-10	1416:1420	The pattern of increase in plasma IL-8 and IL-10 did not differ significantly between conditions (P > 0.05).
29870157	3	34	dep	morning	535:541	arg1	the					531:533	the	531:533	the	531:533	Thirteen young, healthy, recreationally active males performed two experimental days with endurance exercise in the morning and resistance exercise in the afternoon.
29870157	5	35	theme	circulating	847:857	arg1	levels					875:880	circulating plasma cytokine levels	847:880	circulating plasma cytokine levels (e.g. IL-6, IL-8, IL-10, IL-15, TNFα, ANGPTL4, decorin and MCP-1)	847:946	Blood was taken at baseline and at regular time intervals to measure circulating plasma cytokine levels (e.g. IL-6, IL-8, IL-10, IL-15, TNFα, ANGPTL4, decorin and MCP-1).
29870157	0	36	theme	resistance	29:38	arg1	exercise					40:47	resistance exercise	29:47	resistance exercise with different nutrient availability on a concurrent exercise day in trained healthy males	29:138	Plasma cytokine responses to resistance exercise with different nutrient availability on a concurrent exercise day in trained healthy males.
29870157	13	37	theme	circulating	2097:2107	arg1	cytokines					2116:2124	circulating plasma cytokines	2097:2124	circulating plasma cytokines	2097:2124	Our findings support the view that pre-exercise carbohydrate availability does not have a major impact on acute responses of circulating plasma cytokines in humans.
29870157	6	38	theme	resistance	1039:1048	arg1	exercise					1050:1057	the resistance exercise	1035:1057	the resistance exercise on the carbohydrate condition	1035:1087	Plasma glucose and insulin were higher in the postprandial period before the start of the resistance exercise on the carbohydrate condition, while free fatty acids were reduced.
29870157	4	39	theme	resistance	757:766	arg1	exercise					768:775	resistance exercise	757:775	resistance exercise	757:775	Either a carbohydrate (110 g carbohydrate, 52 g protein, 9 g fat; ~750 kcal) or an isocaloric fat meal (20 gr carbohydrate, 52 g protein, 51 g fat) was provided 2 h before resistance exercise.
29870157	2	40	theme	cytokine	331:338	arg1	responses					340:348	plasma cytokine responses	324:348	plasma cytokine responses to resistance exercise after an endurance exercise	324:399	We aimed to evaluate the effect of a preresistance exercise carbohydrate meal versus fat meal on plasma cytokine responses to resistance exercise after an endurance exercise earlier that day.
29870157	13	41	theme	major	2062:2066	arg1	impact					2068:2073	a major impact	2060:2073	a major impact on acute responses of circulating plasma cytokines in humans	2060:2134	Our findings support the view that pre-exercise carbohydrate availability does not have a major impact on acute responses of circulating plasma cytokines in humans.
29870157	3	42	theme	resistance	547:556	arg1	exercise					558:565	resistance exercise	547:565	resistance exercise	547:565	Thirteen young, healthy, recreationally active males performed two experimental days with endurance exercise in the morning and resistance exercise in the afternoon.
29870157	4	43	theme	52 g	628:631	arg1	carbohydrate					614:625	110 g carbohydrate	608:625	110 g carbohydrate	608:625	Either a carbohydrate (110 g carbohydrate, 52 g protein, 9 g fat; ~750 kcal) or an isocaloric fat meal (20 gr carbohydrate, 52 g protein, 51 g fat) was provided 2 h before resistance exercise.
29870157	4	43	theme	52 g	628:631	arg1	protein					633:639	52 g protein	628:639	52 g protein	628:639	Either a carbohydrate (110 g carbohydrate, 52 g protein, 9 g fat; ~750 kcal) or an isocaloric fat meal (20 gr carbohydrate, 52 g protein, 51 g fat) was provided 2 h before resistance exercise.
29870157	7	44	theme	IL-6	1159:1162	arg1	concentrations					1164:1177	IL-6 concentrations	1159:1177	IL-6 concentrations	1159:1177	At 2 h postresistance exercise, IL-6 concentrations were higher in the fat condition compared to the carbohydrate condition (P < 0.05).
29870157	10	45	dep	conditions	1516:1525	arg1	remain					1553:1558	remain	1553:1558	remain constant when compared with baseline (P > 0.05)	1553:1606	There were no differences between conditions on TNFα levels and levels remain constant when compared with baseline (P > 0.05).
29870157	12	46	theme	exercise	1870:1877	arg1	levels					1903:1908	postresistance exercise circulating plasma IL-6 levels	1855:1908	postresistance exercise circulating plasma IL-6 levels	1855:1908	The composition of the pre-exercise meal did in general not influence cytokine responses in the postresistance exercise period, except postresistance exercise circulating plasma IL-6 levels being higher in the fat condition compared with carbohydrate.
29870157	9	47	from	plasma	1400:1405	arg1	pattern					1377:1383	The pattern	1373:1383	The pattern of increase in plasma IL-8 and IL-10	1373:1420	The pattern of increase in plasma IL-8 and IL-10 did not differ significantly between conditions (P > 0.05).
29870157	6	48	theme	free	1096:1099	arg1	acids					1107:1111	free fatty acids	1096:1111	free fatty acids	1096:1111	Plasma glucose and insulin were higher in the postprandial period before the start of the resistance exercise on the carbohydrate condition, while free fatty acids were reduced.
29870157	3	49	from	morning	535:541	arg1	afternoon					574:582	the afternoon	570:582	the afternoon	570:582	Thirteen young, healthy, recreationally active males performed two experimental days with endurance exercise in the morning and resistance exercise in the afternoon.
29870157	2	50	theme	exercise	278:285	arg1	effect					252:257	the effect	248:257	the effect of a preresistance exercise carbohydrate meal versus fat meal on plasma cytokine responses to resistance exercise after an endurance exercise	248:399	We aimed to evaluate the effect of a preresistance exercise carbohydrate meal versus fat meal on plasma cytokine responses to resistance exercise after an endurance exercise earlier that day.
29870157	5	51	theme	time	821:824	arg1	intervals					826:834	regular time intervals	813:834	regular time intervals	813:834	Blood was taken at baseline and at regular time intervals to measure circulating plasma cytokine levels (e.g. IL-6, IL-8, IL-10, IL-15, TNFα, ANGPTL4, decorin and MCP-1).
29870157	12	52	theme	plasma	1891:1896	arg1	levels					1903:1908	postresistance exercise circulating plasma IL-6 levels	1855:1908	postresistance exercise circulating plasma IL-6 levels	1855:1908	The composition of the pre-exercise meal did in general not influence cytokine responses in the postresistance exercise period, except postresistance exercise circulating plasma IL-6 levels being higher in the fat condition compared with carbohydrate.
29870157	9	53	from	increase	1388:1395	arg1	plasma					1400:1405	plasma IL-8 and IL-10	1400:1420	plasma IL-8 and IL-10	1400:1420	The pattern of increase in plasma IL-8 and IL-10 did not differ significantly between conditions (P > 0.05).
29870157	12	54	dep	general	1768:1774	arg1	influence					1780:1788	influence	1780:1788	influence	1780:1788	The composition of the pre-exercise meal did in general not influence cytokine responses in the postresistance exercise period, except postresistance exercise circulating plasma IL-6 levels being higher in the fat condition compared with carbohydrate.
29870157	12	55	theme	fat	1930:1932	arg1	condition					1934:1942	the fat condition	1926:1942	the fat condition	1926:1942	The composition of the pre-exercise meal did in general not influence cytokine responses in the postresistance exercise period, except postresistance exercise circulating plasma IL-6 levels being higher in the fat condition compared with carbohydrate.
29870157	1	56	theme	potential	184:192	arg1	availability					154:165	Carbohydrate availability	141:165	Carbohydrate availability	141:165	Carbohydrate availability is proposed as a potential regulator of cytokine responses.
29870157	1	56	theme	potential	184:192	arg1	regulator					194:202	a potential regulator	182:202	a potential regulator of cytokine responses	182:224	Carbohydrate availability is proposed as a potential regulator of cytokine responses.
29870157	9	57	from	pattern	1377:1383	arg1	plasma					1400:1405	plasma IL-8 and IL-10	1400:1420	plasma IL-8 and IL-10	1400:1420	The pattern of increase in plasma IL-8 and IL-10 did not differ significantly between conditions (P > 0.05).
29870157	6	58	theme	carbohydrate	1066:1077	arg1	condition					1079:1087	the carbohydrate condition	1062:1087	the carbohydrate condition	1062:1087	Plasma glucose and insulin were higher in the postprandial period before the start of the resistance exercise on the carbohydrate condition, while free fatty acids were reduced.
29870157	0	59	theme	healthy	126:132	arg1	males					134:138	trained healthy males	118:138	trained healthy males	118:138	Plasma cytokine responses to resistance exercise with different nutrient availability on a concurrent exercise day in trained healthy males.
29870157	2	60	theme	fat	312:314	arg1	meal					316:319	fat meal	312:319	fat meal	312:319	We aimed to evaluate the effect of a preresistance exercise carbohydrate meal versus fat meal on plasma cytokine responses to resistance exercise after an endurance exercise earlier that day.
29870157	1	61	theme	Carbohydrate	141:152	arg1	availability					154:165	Carbohydrate availability	141:165	Carbohydrate availability	141:165	Carbohydrate availability is proposed as a potential regulator of cytokine responses.
29870157	1	61	theme	Carbohydrate	141:152	arg1	regulator					194:202	a potential regulator	182:202	a potential regulator of cytokine responses	182:224	Carbohydrate availability is proposed as a potential regulator of cytokine responses.
29870157	7	62	theme	fat	1198:1200	arg1	condition					1202:1210	the fat condition	1194:1210	the fat condition	1194:1210	At 2 h postresistance exercise, IL-6 concentrations were higher in the fat condition compared to the carbohydrate condition (P < 0.05).
29870157	13	63	theme	pre-exercise	2007:2018	arg1	availability					2033:2044	pre-exercise carbohydrate availability	2007:2044	pre-exercise carbohydrate availability	2007:2044	Our findings support the view that pre-exercise carbohydrate availability does not have a major impact on acute responses of circulating plasma cytokines in humans.
29870157	1	64	theme	responses	216:224	arg1	availability					154:165	Carbohydrate availability	141:165	Carbohydrate availability	141:165	Carbohydrate availability is proposed as a potential regulator of cytokine responses.
29870157	1	64	theme	responses	216:224	arg1	regulator					194:202	a potential regulator	182:202	a potential regulator of cytokine responses	182:224	Carbohydrate availability is proposed as a potential regulator of cytokine responses.
29870157	12	65	theme	postresistance	1816:1829	arg1	period					1840:1845	the postresistance exercise period	1812:1845	the postresistance exercise period	1812:1845	The composition of the pre-exercise meal did in general not influence cytokine responses in the postresistance exercise period, except postresistance exercise circulating plasma IL-6 levels being higher in the fat condition compared with carbohydrate.
29870157	2	66	dep	exercise	278:285	arg1	meal					300:303	carbohydrate meal	287:303	carbohydrate meal	287:303	We aimed to evaluate the effect of a preresistance exercise carbohydrate meal versus fat meal on plasma cytokine responses to resistance exercise after an endurance exercise earlier that day.
29870157	4	67	theme	20 gr	689:693	arg1	carbohydrate					695:706	20 gr carbohydrate	689:706	20 gr carbohydrate	689:706	Either a carbohydrate (110 g carbohydrate, 52 g protein, 9 g fat; ~750 kcal) or an isocaloric fat meal (20 gr carbohydrate, 52 g protein, 51 g fat) was provided 2 h before resistance exercise.
29870157	4	67	theme	20 gr	689:693	arg1	protein					714:720	52 g protein	709:720	52 g protein	709:720	Either a carbohydrate (110 g carbohydrate, 52 g protein, 9 g fat; ~750 kcal) or an isocaloric fat meal (20 gr carbohydrate, 52 g protein, 51 g fat) was provided 2 h before resistance exercise.
29870157	4	67	theme	20 gr	689:693	arg1	fat					728:730	51 g fat	723:730	51 g fat	723:730	Either a carbohydrate (110 g carbohydrate, 52 g protein, 9 g fat; ~750 kcal) or an isocaloric fat meal (20 gr carbohydrate, 52 g protein, 51 g fat) was provided 2 h before resistance exercise.
29870157	11	68	theme	carbohydrate	1685:1696	arg1	condition					1698:1706	carbohydrate condition	1685:1706	carbohydrate condition	1685:1706	ANGPTL4, IL-15, Decorin and MCP-1 showed no differences between the fat and carbohydrate condition (P > 0.05).
29870157	9	69	theme	increase	1388:1395	arg1	pattern					1377:1383	The pattern	1373:1383	The pattern of increase in plasma IL-8 and IL-10	1373:1420	The pattern of increase in plasma IL-8 and IL-10 did not differ significantly between conditions (P > 0.05).
29870157	6	70	theme	Plasma	949:954	arg1	glucose					956:962	Plasma glucose	949:962	Plasma glucose	949:962	Plasma glucose and insulin were higher in the postprandial period before the start of the resistance exercise on the carbohydrate condition, while free fatty acids were reduced.
29870157	3	71	theme	experimental	486:497	arg1	days					499:502	two experimental days	482:502	two experimental days with endurance exercise	482:526	Thirteen young, healthy, recreationally active males performed two experimental days with endurance exercise in the morning and resistance exercise in the afternoon.
29870157	7	72	theme	postresistance	1134:1147	arg1	exercise					1149:1156	2 h postresistance exercise	1130:1156	2 h postresistance exercise	1130:1156	At 2 h postresistance exercise, IL-6 concentrations were higher in the fat condition compared to the carbohydrate condition (P < 0.05).
29870157	4	73	dep	carbohydrate	614:625	arg1	~750 kcal					651:659	~750 kcal	651:659	~750 kcal	651:659	Either a carbohydrate (110 g carbohydrate, 52 g protein, 9 g fat; ~750 kcal) or an isocaloric fat meal (20 gr carbohydrate, 52 g protein, 51 g fat) was provided 2 h before resistance exercise.
29870157	4	74	dep	2 h	746:748	arg1	exercise					768:775	resistance exercise	757:775	resistance exercise	757:775	Either a carbohydrate (110 g carbohydrate, 52 g protein, 9 g fat; ~750 kcal) or an isocaloric fat meal (20 gr carbohydrate, 52 g protein, 51 g fat) was provided 2 h before resistance exercise.
29870157	4	75	theme	isocaloric	668:677	arg1	meal					683:686	an isocaloric fat meal	665:686	an isocaloric fat meal (20 gr carbohydrate, 52 g protein, 51 g fat)	665:731	Either a carbohydrate (110 g carbohydrate, 52 g protein, 9 g fat; ~750 kcal) or an isocaloric fat meal (20 gr carbohydrate, 52 g protein, 51 g fat) was provided 2 h before resistance exercise.
29870157	5	76	theme	cytokine	866:873	arg1	levels					875:880	circulating plasma cytokine levels	847:880	circulating plasma cytokine levels (e.g. IL-6, IL-8, IL-10, IL-15, TNFα, ANGPTL4, decorin and MCP-1)	847:946	Blood was taken at baseline and at regular time intervals to measure circulating plasma cytokine levels (e.g. IL-6, IL-8, IL-10, IL-15, TNFα, ANGPTL4, decorin and MCP-1).
29870157	3	77	from	exercise	558:565	arg1	afternoon					574:582	the afternoon	570:582	the afternoon	570:582	Thirteen young, healthy, recreationally active males performed two experimental days with endurance exercise in the morning and resistance exercise in the afternoon.
29870157	0	78	theme	exercise	102:109	arg1	day					111:113	a concurrent exercise day	89:113	a concurrent exercise day in trained healthy males	89:138	Plasma cytokine responses to resistance exercise with different nutrient availability on a concurrent exercise day in trained healthy males.
29870157	11	79	dep	fat	1677:1679	arg1	the					1673:1675	the	1673:1675	the	1673:1675	ANGPTL4, IL-15, Decorin and MCP-1 showed no differences between the fat and carbohydrate condition (P > 0.05).
29870157	6	80	theme	postprandial	995:1006	arg1	period					1008:1013	the postprandial period	991:1013	the postprandial period before the start of the resistance exercise on the carbohydrate condition	991:1087	Plasma glucose and insulin were higher in the postprandial period before the start of the resistance exercise on the carbohydrate condition, while free fatty acids were reduced.
29870157	0	81	from	exercise	40:47	arg1	day					111:113	a concurrent exercise day	89:113	a concurrent exercise day in trained healthy males	89:138	Plasma cytokine responses to resistance exercise with different nutrient availability on a concurrent exercise day in trained healthy males.
29870157	0	82	theme	cytokine	7:14	arg1	responses					16:24	Plasma cytokine responses	0:24	Plasma cytokine responses to resistance exercise with different nutrient availability on a concurrent exercise day in trained healthy males.	0:139	Plasma cytokine responses to resistance exercise with different nutrient availability on a concurrent exercise day in trained healthy males.
29870157	7	83	theme	carbohydrate	1228:1239	arg1	condition					1241:1249	the carbohydrate condition	1224:1249	the carbohydrate condition (P < 0.05)	1224:1260	At 2 h postresistance exercise, IL-6 concentrations were higher in the fat condition compared to the carbohydrate condition (P < 0.05).
29870157	7	83	theme	carbohydrate	1228:1239	arg1	P < 0.05					1252:1259	P < 0.05	1252:1259	P < 0.05	1252:1259	At 2 h postresistance exercise, IL-6 concentrations were higher in the fat condition compared to the carbohydrate condition (P < 0.05).
29870157	3	84	dep	young	428:432	arg1	healthy					435:441	healthy	435:441	healthy	435:441	Thirteen young, healthy, recreationally active males performed two experimental days with endurance exercise in the morning and resistance exercise in the afternoon.
29870157	3	84	dep	young	428:432	arg1	active					459:464	active	459:464	active	459:464	Thirteen young, healthy, recreationally active males performed two experimental days with endurance exercise in the morning and resistance exercise in the afternoon.
29870157	2	85	theme	endurance	382:390	arg1	exercise					392:399	an endurance exercise	379:399	an endurance exercise	379:399	We aimed to evaluate the effect of a preresistance exercise carbohydrate meal versus fat meal on plasma cytokine responses to resistance exercise after an endurance exercise earlier that day.
29870157	4	86	theme	51 g	723:726	arg1	carbohydrate					695:706	20 gr carbohydrate	689:706	20 gr carbohydrate	689:706	Either a carbohydrate (110 g carbohydrate, 52 g protein, 9 g fat; ~750 kcal) or an isocaloric fat meal (20 gr carbohydrate, 52 g protein, 51 g fat) was provided 2 h before resistance exercise.
29870157	4	86	theme	51 g	723:726	arg1	fat					728:730	51 g fat	723:730	51 g fat	723:730	Either a carbohydrate (110 g carbohydrate, 52 g protein, 9 g fat; ~750 kcal) or an isocaloric fat meal (20 gr carbohydrate, 52 g protein, 51 g fat) was provided 2 h before resistance exercise.
29870157	6	87	from	condition	1079:1087	arg1	start					1026:1030	the start	1022:1030	the start of the resistance exercise on the carbohydrate condition	1022:1087	Plasma glucose and insulin were higher in the postprandial period before the start of the resistance exercise on the carbohydrate condition, while free fatty acids were reduced.
29870157	12	88	theme	pre-exercise	1743:1754	arg1	meal					1756:1759	the pre-exercise meal	1739:1759	the pre-exercise meal	1739:1759	The composition of the pre-exercise meal did in general not influence cytokine responses in the postresistance exercise period, except postresistance exercise circulating plasma IL-6 levels being higher in the fat condition compared with carbohydrate.
29870157	3	89	theme	young	428:432	arg1	males					466:470	Thirteen young, healthy, recreationally active males	419:470	Thirteen young, healthy, recreationally active males	419:470	Thirteen young, healthy, recreationally active males performed two experimental days with endurance exercise in the morning and resistance exercise in the afternoon.
29870157	8	90	theme	IL-6	1295:1298	arg1	levels					1300:1305	IL-6 levels	1295:1305	IL-6 levels	1295:1305	In addition, in both conditions IL-6 levels were higher at all time points compared with baseline (P < 0.05).
29870157	0	91	theme	different	54:62	arg1	availability					73:84	different nutrient availability	54:84	different nutrient availability	54:84	Plasma cytokine responses to resistance exercise with different nutrient availability on a concurrent exercise day in trained healthy males.
29870157	6	92	theme	exercise	1050:1057	arg1	start					1026:1030	the start	1022:1030	the start of the resistance exercise on the carbohydrate condition	1022:1087	Plasma glucose and insulin were higher in the postprandial period before the start of the resistance exercise on the carbohydrate condition, while free fatty acids were reduced.
29870157	6	93	from	exercise	1050:1057	arg1	condition					1079:1087	the carbohydrate condition	1062:1087	the carbohydrate condition	1062:1087	Plasma glucose and insulin were higher in the postprandial period before the start of the resistance exercise on the carbohydrate condition, while free fatty acids were reduced.
29870157	3	94	theme	endurance	509:517	arg1	exercise					519:526	endurance exercise	509:526	endurance exercise	509:526	Thirteen young, healthy, recreationally active males performed two experimental days with endurance exercise in the morning and resistance exercise in the afternoon.
29870157	13	95	theme	plasma	2109:2114	arg1	cytokines					2116:2124	circulating plasma cytokines	2097:2124	circulating plasma cytokines	2097:2124	Our findings support the view that pre-exercise carbohydrate availability does not have a major impact on acute responses of circulating plasma cytokines in humans.
29870157	0	96	with	exercise	40:47	arg1	availability					73:84	different nutrient availability	54:84	different nutrient availability	54:84	Plasma cytokine responses to resistance exercise with different nutrient availability on a concurrent exercise day in trained healthy males.
29870157	13	97	from	impact	2068:2073	arg1	responses					2084:2092	acute responses	2078:2092	acute responses of circulating plasma cytokines in humans	2078:2134	Our findings support the view that pre-exercise carbohydrate availability does not have a major impact on acute responses of circulating plasma cytokines in humans.
29870157	2	98	theme	plasma	324:329	arg1	responses					340:348	plasma cytokine responses	324:348	plasma cytokine responses to resistance exercise after an endurance exercise	324:399	We aimed to evaluate the effect of a preresistance exercise carbohydrate meal versus fat meal on plasma cytokine responses to resistance exercise after an endurance exercise earlier that day.
29870157	13	99	contain	have	2055:2058	arg2	impact					2068:2073	a major impact	2060:2073	a major impact on acute responses of circulating plasma cytokines in humans	2060:2134	Our findings support the view that pre-exercise carbohydrate availability does not have a major impact on acute responses of circulating plasma cytokines in humans.
29870157	13	99	contain	have	2055:2058	arg1	availability					2033:2044	pre-exercise carbohydrate availability	2007:2044	pre-exercise carbohydrate availability	2007:2044	Our findings support the view that pre-exercise carbohydrate availability does not have a major impact on acute responses of circulating plasma cytokines in humans.
29870157	8	100	theme	time	1326:1329	arg1	points					1331:1336	all time points	1322:1336	all time points	1322:1336	In addition, in both conditions IL-6 levels were higher at all time points compared with baseline (P < 0.05).
29870157	6	101	from	start	1026:1030	arg1	condition					1079:1087	the carbohydrate condition	1062:1087	the carbohydrate condition	1062:1087	Plasma glucose and insulin were higher in the postprandial period before the start of the resistance exercise on the carbohydrate condition, while free fatty acids were reduced.
29870157	13	102	theme	acute	2078:2082	arg1	responses					2084:2092	acute responses	2078:2092	acute responses of circulating plasma cytokines in humans	2078:2134	Our findings support the view that pre-exercise carbohydrate availability does not have a major impact on acute responses of circulating plasma cytokines in humans.
29981277	8	0	theme	tissue-specific	1739:1753	arg1	composition					1764:1774	tissue-specific hydrogel composition	1739:1774	tissue-specific hydrogel composition	1739:1774	We discussed and concluded that osteogenic and angiogenic signals from the culture media as well as from cocultured cell types, and tissue-specific hydrogel composition all contribute to stimulate the interplay between osteogenesis and angiogenesis in vitro and are a basis for engineering vascularized bone.
29981277	3	1	theme	soft	760:763	arg1	gels					768:771	soft GM gels	760:771	soft GM gels	760:771	HDMECs, together with hASCs as supporting cells, were encapsulated in soft GM gels and were indirectly cocultured with hASCs encapsulated in stiffer GM hydrogels additionally containing methacrylate-modified hyaluronic acid and hydroxyapatite particles.
29981277	2	2	theme	coculture	379:387	arg1	system					389:394	a coculture system	377:394	a coculture system combining primary human adipose-derived stem cells (hASCs) and primary human dermal microvascular endothelial cells (HDMECs) within two types of hydrogels based on methacryloyl-modified gelatin (GM) as three-dimensional scaffolds	377:624	In this study, a coculture system combining primary human adipose-derived stem cells (hASCs) and primary human dermal microvascular endothelial cells (HDMECs) within two types of hydrogels based on methacryloyl-modified gelatin (GM) as three-dimensional scaffolds was examined for its support of tissue specific cell functions.
29981277	7	3	theme	vascular	1547:1554	arg1	networks					1556:1563	the vascular networks	1543:1563	the vascular networks	1543:1563	In addition, the stability as well as the complexity of the vascular networks was significantly increased by coculture.
29981277	0	4	theme	cells	83:87	arg1	coculture					58:66	coculture	58:66	coculture of endothelial cells and stem cells in tissue-specific methacryloyl gelatin-based hydrogels	58:158	Improved vasculogenesis and bone matrix formation through coculture of endothelial cells and stem cells in tissue-specific methacryloyl gelatin-based hydrogels.
29981277	6	5	from	structures	1455:1464	arg1	gels					1481:1484	the control gels	1469:1484	the control gels	1469:1484	Within the soft vascularization gels, the formed capillary-like networks were significantly longer after 14 days of coculture than the structures in the control gels.
29981277	1	6	theme	osteogenic	178:187	arg1	cells					204:208	osteogenic and angiogenic cells	178:208	osteogenic and angiogenic cells	178:208	The coculture of osteogenic and angiogenic cells and the resulting paracrine signaling via soluble factors are supposed to be crucial for successfully engineering vascularized bone tissue equivalents.
29981277	3	7	theme	hydroxyapatite	918:931	arg1	particles					933:941	hydroxyapatite particles	918:941	hydroxyapatite particles	918:941	HDMECs, together with hASCs as supporting cells, were encapsulated in soft GM gels and were indirectly cocultured with hASCs encapsulated in stiffer GM hydrogels additionally containing methacrylate-modified hyaluronic acid and hydroxyapatite particles.
29981277	8	8	theme	angiogenic	1654:1663	arg1	signals					1665:1671	osteogenic and angiogenic signals	1639:1671	osteogenic and angiogenic signals from the culture media as well as from cocultured cell types,	1639:1733	We discussed and concluded that osteogenic and angiogenic signals from the culture media as well as from cocultured cell types, and tissue-specific hydrogel composition all contribute to stimulate the interplay between osteogenesis and angiogenesis in vitro and are a basis for engineering vascularized bone.
29981277	0	9	theme	cells	98:102	arg1	coculture					58:66	coculture	58:66	coculture of endothelial cells and stem cells in tissue-specific methacryloyl gelatin-based hydrogels	58:158	Improved vasculogenesis and bone matrix formation through coculture of endothelial cells and stem cells in tissue-specific methacryloyl gelatin-based hydrogels.
29981277	2	10	theme	functions	679:687	arg1	support					647:653	its support	643:653	its support of tissue specific cell functions	643:687	In this study, a coculture system combining primary human adipose-derived stem cells (hASCs) and primary human dermal microvascular endothelial cells (HDMECs) within two types of hydrogels based on methacryloyl-modified gelatin (GM) as three-dimensional scaffolds was examined for its support of tissue specific cell functions.
29981277	8	11	theme	cocultured	1712:1721	arg1	types					1728:1732	cocultured cell types	1712:1732	cocultured cell types	1712:1732	We discussed and concluded that osteogenic and angiogenic signals from the culture media as well as from cocultured cell types, and tissue-specific hydrogel composition all contribute to stimulate the interplay between osteogenesis and angiogenesis in vitro and are a basis for engineering vascularized bone.
29981277	3	12	theme	GM	839:840	arg1	hydrogels					842:850	stiffer GM hydrogels	831:850	stiffer GM hydrogels additionally containing methacrylate-modified hyaluronic acid and hydroxyapatite particles	831:941	HDMECs, together with hASCs as supporting cells, were encapsulated in soft GM gels and were indirectly cocultured with hASCs encapsulated in stiffer GM hydrogels additionally containing methacrylate-modified hyaluronic acid and hydroxyapatite particles.
29981277	0	13	theme	stem	93:96	arg1	cells					98:102	stem cells	93:102	stem cells	93:102	Improved vasculogenesis and bone matrix formation through coculture of endothelial cells and stem cells in tissue-specific methacryloyl gelatin-based hydrogels.
29981277	0	14	from	coculture	58:66	arg1	hydrogels					150:158	tissue-specific methacryloyl gelatin-based hydrogels	107:158	tissue-specific methacryloyl gelatin-based hydrogels	107:158	Improved vasculogenesis and bone matrix formation through coculture of endothelial cells and stem cells in tissue-specific methacryloyl gelatin-based hydrogels.
29981277	2	15	theme	cell	674:677	arg1	functions					679:687	tissue specific cell functions	658:687	tissue specific cell functions	658:687	In this study, a coculture system combining primary human adipose-derived stem cells (hASCs) and primary human dermal microvascular endothelial cells (HDMECs) within two types of hydrogels based on methacryloyl-modified gelatin (GM) as three-dimensional scaffolds was examined for its support of tissue specific cell functions.
29981277	5	16	from	gels	1299:1302	arg1	monoculture					1307:1317	monoculture	1307:1317	monoculture	1307:1317	After 14 days of coculture with HDMEC-laden hydrogels, the viscoelastic properties of the bone gels were significantly higher compared with the gels in monoculture.
29981277	5	17	theme	bone	1245:1248	arg1	gels					1250:1253	the bone gels	1241:1253	the bone gels	1241:1253	After 14 days of coculture with HDMEC-laden hydrogels, the viscoelastic properties of the bone gels were significantly higher compared with the gels in monoculture.
29981277	0	18	theme	tissue-specific	107:121	arg1	hydrogels					150:158	tissue-specific methacryloyl gelatin-based hydrogels	107:158	tissue-specific methacryloyl gelatin-based hydrogels	107:158	Improved vasculogenesis and bone matrix formation through coculture of endothelial cells and stem cells in tissue-specific methacryloyl gelatin-based hydrogels.
29981277	6	19	theme	coculture	1436:1444	arg1	days					1428:1431	14 days	1425:1431	14 days of coculture	1425:1444	Within the soft vascularization gels, the formed capillary-like networks were significantly longer after 14 days of coculture than the structures in the control gels.
29981277	2	20	theme	tissue	658:663	arg1	functions					679:687	tissue specific cell functions	658:687	tissue specific cell functions	658:687	In this study, a coculture system combining primary human adipose-derived stem cells (hASCs) and primary human dermal microvascular endothelial cells (HDMECs) within two types of hydrogels based on methacryloyl-modified gelatin (GM) as three-dimensional scaffolds was examined for its support of tissue specific cell functions.
29981277	2	21	theme	specific	665:672	arg1	functions					679:687	tissue specific cell functions	658:687	tissue specific cell functions	658:687	In this study, a coculture system combining primary human adipose-derived stem cells (hASCs) and primary human dermal microvascular endothelial cells (HDMECs) within two types of hydrogels based on methacryloyl-modified gelatin (GM) as three-dimensional scaffolds was examined for its support of tissue specific cell functions.
29981277	8	22	from	media	1690:1694	arg1	signals					1665:1671	osteogenic and angiogenic signals	1639:1671	osteogenic and angiogenic signals from the culture media as well as from cocultured cell types,	1639:1733	We discussed and concluded that osteogenic and angiogenic signals from the culture media as well as from cocultured cell types, and tissue-specific hydrogel composition all contribute to stimulate the interplay between osteogenesis and angiogenesis in vitro and are a basis for engineering vascularized bone.
29981277	8	22	from	media	1690:1694	arg1	composition					1764:1774	tissue-specific hydrogel composition	1739:1774	tissue-specific hydrogel composition	1739:1774	We discussed and concluded that osteogenic and angiogenic signals from the culture media as well as from cocultured cell types, and tissue-specific hydrogel composition all contribute to stimulate the interplay between osteogenesis and angiogenesis in vitro and are a basis for engineering vascularized bone.
29981277	2	23	theme	human	414:418	arg1	hASCs					448:452	hASCs	448:452	hASCs	448:452	In this study, a coculture system combining primary human adipose-derived stem cells (hASCs) and primary human dermal microvascular endothelial cells (HDMECs) within two types of hydrogels based on methacryloyl-modified gelatin (GM) as three-dimensional scaffolds was examined for its support of tissue specific cell functions.
29981277	2	23	theme	human	414:418	arg1	cells					441:445	primary human adipose-derived stem cells	406:445	primary human adipose-derived stem cells (hASCs)	406:453	In this study, a coculture system combining primary human adipose-derived stem cells (hASCs) and primary human dermal microvascular endothelial cells (HDMECs) within two types of hydrogels based on methacryloyl-modified gelatin (GM) as three-dimensional scaffolds was examined for its support of tissue specific cell functions.
29981277	2	24	theme	stem	436:439	arg1	hASCs					448:452	hASCs	448:452	hASCs	448:452	In this study, a coculture system combining primary human adipose-derived stem cells (hASCs) and primary human dermal microvascular endothelial cells (HDMECs) within two types of hydrogels based on methacryloyl-modified gelatin (GM) as three-dimensional scaffolds was examined for its support of tissue specific cell functions.
29981277	2	24	theme	stem	436:439	arg1	cells					441:445	primary human adipose-derived stem cells	406:445	primary human adipose-derived stem cells (hASCs)	406:453	In this study, a coculture system combining primary human adipose-derived stem cells (hASCs) and primary human dermal microvascular endothelial cells (HDMECs) within two types of hydrogels based on methacryloyl-modified gelatin (GM) as three-dimensional scaffolds was examined for its support of tissue specific cell functions.
29981277	4	25	theme	matrix	1029:1034	arg1	proteins					1036:1043	matrix proteins	1029:1043	matrix proteins like collagen type I and fibronectin	1029:1080	After 14 days, the hASC in the stiffer gels (constituting the "bone gels") expressed matrix proteins like collagen type I and fibronectin, as well as bone-specific proteins osteopontin and alkaline phosphatase.
29981277	4	26	dep	proteins	1108:1115	arg1	osteopontin					1117:1127	osteopontin	1117:1127	osteopontin	1117:1127	After 14 days, the hASC in the stiffer gels (constituting the "bone gels") expressed matrix proteins like collagen type I and fibronectin, as well as bone-specific proteins osteopontin and alkaline phosphatase.
29981277	4	26	dep	proteins	1108:1115	arg1	phosphatase					1142:1152	alkaline phosphatase	1133:1152	alkaline phosphatase	1133:1152	After 14 days, the hASC in the stiffer gels (constituting the "bone gels") expressed matrix proteins like collagen type I and fibronectin, as well as bone-specific proteins osteopontin and alkaline phosphatase.
29981277	4	26	dep	proteins	1108:1115	arg1	proteins					1108:1115	bone-specific proteins	1094:1115	bone-specific proteins osteopontin and alkaline phosphatase	1094:1152	After 14 days, the hASC in the stiffer gels (constituting the "bone gels") expressed matrix proteins like collagen type I and fibronectin, as well as bone-specific proteins osteopontin and alkaline phosphatase.
29981277	1	27	theme	soluble	252:258	arg1	factors					260:266	soluble factors	252:266	soluble factors	252:266	The coculture of osteogenic and angiogenic cells and the resulting paracrine signaling via soluble factors are supposed to be crucial for successfully engineering vascularized bone tissue equivalents.
29981277	4	28	theme	stiffer	975:981	arg1	gels					983:986	the stiffer gels	971:986	the stiffer gels	971:986	After 14 days, the hASC in the stiffer gels (constituting the "bone gels") expressed matrix proteins like collagen type I and fibronectin, as well as bone-specific proteins osteopontin and alkaline phosphatase.
29981277	1	29	theme	angiogenic	193:202	arg1	cells					204:208	osteogenic and angiogenic cells	178:208	osteogenic and angiogenic cells	178:208	The coculture of osteogenic and angiogenic cells and the resulting paracrine signaling via soluble factors are supposed to be crucial for successfully engineering vascularized bone tissue equivalents.
29981277	2	30	theme	dermal	473:478	arg1	HDMECs					513:518	HDMECs	513:518	HDMECs	513:518	In this study, a coculture system combining primary human adipose-derived stem cells (hASCs) and primary human dermal microvascular endothelial cells (HDMECs) within two types of hydrogels based on methacryloyl-modified gelatin (GM) as three-dimensional scaffolds was examined for its support of tissue specific cell functions.
29981277	2	30	theme	dermal	473:478	arg1	cells					506:510	primary human dermal microvascular endothelial cells	459:510	primary human dermal microvascular endothelial cells (HDMECs)	459:519	In this study, a coculture system combining primary human adipose-derived stem cells (hASCs) and primary human dermal microvascular endothelial cells (HDMECs) within two types of hydrogels based on methacryloyl-modified gelatin (GM) as three-dimensional scaffolds was examined for its support of tissue specific cell functions.
29981277	5	31	theme	coculture	1172:1180	arg1	days					1164:1167	14 days	1161:1167	14 days of coculture with HDMEC-laden hydrogels	1161:1207	After 14 days of coculture with HDMEC-laden hydrogels, the viscoelastic properties of the bone gels were significantly higher compared with the gels in monoculture.
29981277	4	32	theme	bone	1007:1010	arg1	gels					1012:1015	the "bone gels	1002:1015	the "bone gels"	1002:1016	After 14 days, the hASC in the stiffer gels (constituting the "bone gels") expressed matrix proteins like collagen type I and fibronectin, as well as bone-specific proteins osteopontin and alkaline phosphatase.
29981277	6	33	theme	vascularization	1336:1350	arg1	gels					1352:1355	the soft vascularization gels	1327:1355	the soft vascularization gels	1327:1355	Within the soft vascularization gels, the formed capillary-like networks were significantly longer after 14 days of coculture than the structures in the control gels.
29981277	1	34	theme	cells	204:208	arg1	coculture					165:173	The coculture	161:173	The coculture of osteogenic and angiogenic cells	161:208	The coculture of osteogenic and angiogenic cells and the resulting paracrine signaling via soluble factors are supposed to be crucial for successfully engineering vascularized bone tissue equivalents.
29981277	1	34	theme	cells	204:208	arg1	crucial					287:293	crucial	287:293	crucial	287:293	The coculture of osteogenic and angiogenic cells and the resulting paracrine signaling via soluble factors are supposed to be crucial for successfully engineering vascularized bone tissue equivalents.
29981277	1	34	theme	cells	204:208	arg1	signaling					238:246	the resulting paracrine signaling	214:246	the resulting paracrine signaling via soluble factors	214:266	The coculture of osteogenic and angiogenic cells and the resulting paracrine signaling via soluble factors are supposed to be crucial for successfully engineering vascularized bone tissue equivalents.
29981277	2	35	theme	human	467:471	arg1	HDMECs					513:518	HDMECs	513:518	HDMECs	513:518	In this study, a coculture system combining primary human adipose-derived stem cells (hASCs) and primary human dermal microvascular endothelial cells (HDMECs) within two types of hydrogels based on methacryloyl-modified gelatin (GM) as three-dimensional scaffolds was examined for its support of tissue specific cell functions.
29981277	2	35	theme	human	467:471	arg1	cells					506:510	primary human dermal microvascular endothelial cells	459:510	primary human dermal microvascular endothelial cells (HDMECs)	459:519	In this study, a coculture system combining primary human adipose-derived stem cells (hASCs) and primary human dermal microvascular endothelial cells (HDMECs) within two types of hydrogels based on methacryloyl-modified gelatin (GM) as three-dimensional scaffolds was examined for its support of tissue specific cell functions.
29981277	6	36	theme	capillary-like	1369:1382	arg1	networks					1384:1391	the formed capillary-like networks	1358:1391	the formed capillary-like networks	1358:1391	Within the soft vascularization gels, the formed capillary-like networks were significantly longer after 14 days of coculture than the structures in the control gels.
29981277	6	36	theme	capillary-like	1369:1382	arg1	longer					1412:1417	longer	1412:1417	longer	1412:1417	Within the soft vascularization gels, the formed capillary-like networks were significantly longer after 14 days of coculture than the structures in the control gels.
29981277	6	37	theme	formed	1362:1367	arg1	networks					1384:1391	the formed capillary-like networks	1358:1391	the formed capillary-like networks	1358:1391	Within the soft vascularization gels, the formed capillary-like networks were significantly longer after 14 days of coculture than the structures in the control gels.
29981277	6	37	theme	formed	1362:1367	arg1	longer					1412:1417	longer	1412:1417	longer	1412:1417	Within the soft vascularization gels, the formed capillary-like networks were significantly longer after 14 days of coculture than the structures in the control gels.
29981277	2	38	theme	primary	406:412	arg1	hASCs					448:452	hASCs	448:452	hASCs	448:452	In this study, a coculture system combining primary human adipose-derived stem cells (hASCs) and primary human dermal microvascular endothelial cells (HDMECs) within two types of hydrogels based on methacryloyl-modified gelatin (GM) as three-dimensional scaffolds was examined for its support of tissue specific cell functions.
29981277	2	38	theme	primary	406:412	arg1	cells					441:445	primary human adipose-derived stem cells	406:445	primary human adipose-derived stem cells (hASCs)	406:453	In this study, a coculture system combining primary human adipose-derived stem cells (hASCs) and primary human dermal microvascular endothelial cells (HDMECs) within two types of hydrogels based on methacryloyl-modified gelatin (GM) as three-dimensional scaffolds was examined for its support of tissue specific cell functions.
29981277	0	39	theme	bone	28:31	arg1	formation					40:48	bone matrix formation	28:48	bone matrix formation	28:48	Improved vasculogenesis and bone matrix formation through coculture of endothelial cells and stem cells in tissue-specific methacryloyl gelatin-based hydrogels.
29981277	8	40	theme	vascularized	1897:1908	arg1	bone					1910:1913	vascularized bone	1897:1913	vascularized bone	1897:1913	We discussed and concluded that osteogenic and angiogenic signals from the culture media as well as from cocultured cell types, and tissue-specific hydrogel composition all contribute to stimulate the interplay between osteogenesis and angiogenesis in vitro and are a basis for engineering vascularized bone.
29981277	0	41	theme	gelatin-based	136:148	arg1	hydrogels					150:158	tissue-specific methacryloyl gelatin-based hydrogels	107:158	tissue-specific methacryloyl gelatin-based hydrogels	107:158	Improved vasculogenesis and bone matrix formation through coculture of endothelial cells and stem cells in tissue-specific methacryloyl gelatin-based hydrogels.
29981277	5	42	with	coculture	1172:1180	arg1	hydrogels					1199:1207	HDMEC-laden hydrogels	1187:1207	HDMEC-laden hydrogels	1187:1207	After 14 days of coculture with HDMEC-laden hydrogels, the viscoelastic properties of the bone gels were significantly higher compared with the gels in monoculture.
29981277	8	43	theme	hydrogel	1755:1762	arg1	composition					1764:1774	tissue-specific hydrogel composition	1739:1774	tissue-specific hydrogel composition	1739:1774	We discussed and concluded that osteogenic and angiogenic signals from the culture media as well as from cocultured cell types, and tissue-specific hydrogel composition all contribute to stimulate the interplay between osteogenesis and angiogenesis in vitro and are a basis for engineering vascularized bone.
29981277	1	44	theme	engineering	312:322	arg1	equivalents					349:359	successfully engineering vascularized bone tissue equivalents	299:359	successfully engineering vascularized bone tissue equivalents	299:359	The coculture of osteogenic and angiogenic cells and the resulting paracrine signaling via soluble factors are supposed to be crucial for successfully engineering vascularized bone tissue equivalents.
29981277	0	45	theme	methacryloyl	123:134	arg1	hydrogels					150:158	tissue-specific methacryloyl gelatin-based hydrogels	107:158	tissue-specific methacryloyl gelatin-based hydrogels	107:158	Improved vasculogenesis and bone matrix formation through coculture of endothelial cells and stem cells in tissue-specific methacryloyl gelatin-based hydrogels.
29981277	7	46	theme	networks	1556:1563	arg1	complexity					1529:1538	the complexity	1525:1538	the stability as well as the complexity of the vascular networks	1500:1563	In addition, the stability as well as the complexity of the vascular networks was significantly increased by coculture.
29981277	7	46	theme	networks	1556:1563	arg1	stability					1504:1512	the stability	1500:1512	the stability as well as the complexity of the vascular networks	1500:1563	In addition, the stability as well as the complexity of the vascular networks was significantly increased by coculture.
29981277	4	47	theme	alkaline	1133:1140	arg1	phosphatase					1142:1152	alkaline phosphatase	1133:1152	alkaline phosphatase	1133:1152	After 14 days, the hASC in the stiffer gels (constituting the "bone gels") expressed matrix proteins like collagen type I and fibronectin, as well as bone-specific proteins osteopontin and alkaline phosphatase.
29981277	4	47	theme	alkaline	1133:1140	arg1	proteins					1108:1115	bone-specific proteins	1094:1115	bone-specific proteins osteopontin and alkaline phosphatase	1094:1152	After 14 days, the hASC in the stiffer gels (constituting the "bone gels") expressed matrix proteins like collagen type I and fibronectin, as well as bone-specific proteins osteopontin and alkaline phosphatase.
29981277	1	48	theme	resulting	218:226	arg1	crucial					287:293	crucial	287:293	crucial	287:293	The coculture of osteogenic and angiogenic cells and the resulting paracrine signaling via soluble factors are supposed to be crucial for successfully engineering vascularized bone tissue equivalents.
29981277	1	48	theme	resulting	218:226	arg1	signaling					238:246	the resulting paracrine signaling	214:246	the resulting paracrine signaling via soluble factors	214:266	The coculture of osteogenic and angiogenic cells and the resulting paracrine signaling via soluble factors are supposed to be crucial for successfully engineering vascularized bone tissue equivalents.
29981277	5	49	theme	viscoelastic	1214:1225	arg1	properties					1227:1236	the viscoelastic properties	1210:1236	the viscoelastic properties of the bone gels	1210:1253	After 14 days of coculture with HDMEC-laden hydrogels, the viscoelastic properties of the bone gels were significantly higher compared with the gels in monoculture.
29981277	5	49	theme	viscoelastic	1214:1225	arg1	higher					1274:1279	higher	1274:1279	higher	1274:1279	After 14 days of coculture with HDMEC-laden hydrogels, the viscoelastic properties of the bone gels were significantly higher compared with the gels in monoculture.
29981277	3	50	theme	GM	765:766	arg1	gels					768:771	soft GM gels	760:771	soft GM gels	760:771	HDMECs, together with hASCs as supporting cells, were encapsulated in soft GM gels and were indirectly cocultured with hASCs encapsulated in stiffer GM hydrogels additionally containing methacrylate-modified hyaluronic acid and hydroxyapatite particles.
29981277	2	51	theme	methacryloyl-modified	560:580	arg1	gelatin					582:588	methacryloyl-modified gelatin	560:588	methacryloyl-modified gelatin (GM)	560:593	In this study, a coculture system combining primary human adipose-derived stem cells (hASCs) and primary human dermal microvascular endothelial cells (HDMECs) within two types of hydrogels based on methacryloyl-modified gelatin (GM) as three-dimensional scaffolds was examined for its support of tissue specific cell functions.
29981277	2	51	theme	methacryloyl-modified	560:580	arg1	GM					591:592	GM	591:592	GM	591:592	In this study, a coculture system combining primary human adipose-derived stem cells (hASCs) and primary human dermal microvascular endothelial cells (HDMECs) within two types of hydrogels based on methacryloyl-modified gelatin (GM) as three-dimensional scaffolds was examined for its support of tissue specific cell functions.
29981277	0	52	from	cells	83:87	arg1	hydrogels					150:158	tissue-specific methacryloyl gelatin-based hydrogels	107:158	tissue-specific methacryloyl gelatin-based hydrogels	107:158	Improved vasculogenesis and bone matrix formation through coculture of endothelial cells and stem cells in tissue-specific methacryloyl gelatin-based hydrogels.
29981277	3	53	theme	hyaluronic	898:907	arg1	acid					909:912	methacrylate-modified hyaluronic acid	876:912	methacrylate-modified hyaluronic acid	876:912	HDMECs, together with hASCs as supporting cells, were encapsulated in soft GM gels and were indirectly cocultured with hASCs encapsulated in stiffer GM hydrogels additionally containing methacrylate-modified hyaluronic acid and hydroxyapatite particles.
29981277	1	54	theme	paracrine	228:236	arg1	crucial					287:293	crucial	287:293	crucial	287:293	The coculture of osteogenic and angiogenic cells and the resulting paracrine signaling via soluble factors are supposed to be crucial for successfully engineering vascularized bone tissue equivalents.
29981277	1	54	theme	paracrine	228:236	arg1	signaling					238:246	the resulting paracrine signaling	214:246	the resulting paracrine signaling via soluble factors	214:266	The coculture of osteogenic and angiogenic cells and the resulting paracrine signaling via soluble factors are supposed to be crucial for successfully engineering vascularized bone tissue equivalents.
29981277	0	55	theme	matrix	33:38	arg1	formation					40:48	bone matrix formation	28:48	bone matrix formation	28:48	Improved vasculogenesis and bone matrix formation through coculture of endothelial cells and stem cells in tissue-specific methacryloyl gelatin-based hydrogels.
29981277	1	56	theme	bone	337:340	arg1	equivalents					349:359	successfully engineering vascularized bone tissue equivalents	299:359	successfully engineering vascularized bone tissue equivalents	299:359	The coculture of osteogenic and angiogenic cells and the resulting paracrine signaling via soluble factors are supposed to be crucial for successfully engineering vascularized bone tissue equivalents.
29981277	2	57	theme	primary	459:465	arg1	HDMECs					513:518	HDMECs	513:518	HDMECs	513:518	In this study, a coculture system combining primary human adipose-derived stem cells (hASCs) and primary human dermal microvascular endothelial cells (HDMECs) within two types of hydrogels based on methacryloyl-modified gelatin (GM) as three-dimensional scaffolds was examined for its support of tissue specific cell functions.
29981277	2	57	theme	primary	459:465	arg1	cells					506:510	primary human dermal microvascular endothelial cells	459:510	primary human dermal microvascular endothelial cells (HDMECs)	459:519	In this study, a coculture system combining primary human adipose-derived stem cells (hASCs) and primary human dermal microvascular endothelial cells (HDMECs) within two types of hydrogels based on methacryloyl-modified gelatin (GM) as three-dimensional scaffolds was examined for its support of tissue specific cell functions.
29981277	2	58	theme	endothelial	494:504	arg1	HDMECs					513:518	HDMECs	513:518	HDMECs	513:518	In this study, a coculture system combining primary human adipose-derived stem cells (hASCs) and primary human dermal microvascular endothelial cells (HDMECs) within two types of hydrogels based on methacryloyl-modified gelatin (GM) as three-dimensional scaffolds was examined for its support of tissue specific cell functions.
29981277	2	58	theme	endothelial	494:504	arg1	cells					506:510	primary human dermal microvascular endothelial cells	459:510	primary human dermal microvascular endothelial cells (HDMECs)	459:519	In this study, a coculture system combining primary human adipose-derived stem cells (hASCs) and primary human dermal microvascular endothelial cells (HDMECs) within two types of hydrogels based on methacryloyl-modified gelatin (GM) as three-dimensional scaffolds was examined for its support of tissue specific cell functions.
29981277	1	59	theme	tissue	342:347	arg1	equivalents					349:359	successfully engineering vascularized bone tissue equivalents	299:359	successfully engineering vascularized bone tissue equivalents	299:359	The coculture of osteogenic and angiogenic cells and the resulting paracrine signaling via soluble factors are supposed to be crucial for successfully engineering vascularized bone tissue equivalents.
29981277	2	60	theme	three-dimensional	598:614	arg1	scaffolds					616:624	three-dimensional scaffolds	598:624	three-dimensional scaffolds	598:624	In this study, a coculture system combining primary human adipose-derived stem cells (hASCs) and primary human dermal microvascular endothelial cells (HDMECs) within two types of hydrogels based on methacryloyl-modified gelatin (GM) as three-dimensional scaffolds was examined for its support of tissue specific cell functions.
29981277	2	61	link	adipose-derived	420:434	arg1	hASCs					448:452	hASCs	448:452	hASCs	448:452	In this study, a coculture system combining primary human adipose-derived stem cells (hASCs) and primary human dermal microvascular endothelial cells (HDMECs) within two types of hydrogels based on methacryloyl-modified gelatin (GM) as three-dimensional scaffolds was examined for its support of tissue specific cell functions.
29981277	2	61	link	adipose-derived	420:434	arg1	cells					441:445	primary human adipose-derived stem cells	406:445	primary human adipose-derived stem cells (hASCs)	406:453	In this study, a coculture system combining primary human adipose-derived stem cells (hASCs) and primary human dermal microvascular endothelial cells (HDMECs) within two types of hydrogels based on methacryloyl-modified gelatin (GM) as three-dimensional scaffolds was examined for its support of tissue specific cell functions.
29981277	3	62	contain	containing	865:874	arg1	hydrogels					842:850	stiffer GM hydrogels	831:850	stiffer GM hydrogels additionally containing methacrylate-modified hyaluronic acid and hydroxyapatite particles	831:941	HDMECs, together with hASCs as supporting cells, were encapsulated in soft GM gels and were indirectly cocultured with hASCs encapsulated in stiffer GM hydrogels additionally containing methacrylate-modified hyaluronic acid and hydroxyapatite particles.
29981277	3	62	contain	containing	865:874	arg2	particles					933:941	hydroxyapatite particles	918:941	hydroxyapatite particles	918:941	HDMECs, together with hASCs as supporting cells, were encapsulated in soft GM gels and were indirectly cocultured with hASCs encapsulated in stiffer GM hydrogels additionally containing methacrylate-modified hyaluronic acid and hydroxyapatite particles.
29981277	3	62	contain	containing	865:874	arg2	acid					909:912	methacrylate-modified hyaluronic acid	876:912	methacrylate-modified hyaluronic acid	876:912	HDMECs, together with hASCs as supporting cells, were encapsulated in soft GM gels and were indirectly cocultured with hASCs encapsulated in stiffer GM hydrogels additionally containing methacrylate-modified hyaluronic acid and hydroxyapatite particles.
29981277	6	63	theme	control	1473:1479	arg1	gels					1481:1484	the control gels	1469:1484	the control gels	1469:1484	Within the soft vascularization gels, the formed capillary-like networks were significantly longer after 14 days of coculture than the structures in the control gels.
29981277	2	64	theme	microvascular	480:492	arg1	HDMECs					513:518	HDMECs	513:518	HDMECs	513:518	In this study, a coculture system combining primary human adipose-derived stem cells (hASCs) and primary human dermal microvascular endothelial cells (HDMECs) within two types of hydrogels based on methacryloyl-modified gelatin (GM) as three-dimensional scaffolds was examined for its support of tissue specific cell functions.
29981277	2	64	theme	microvascular	480:492	arg1	cells					506:510	primary human dermal microvascular endothelial cells	459:510	primary human dermal microvascular endothelial cells (HDMECs)	459:519	In this study, a coculture system combining primary human adipose-derived stem cells (hASCs) and primary human dermal microvascular endothelial cells (HDMECs) within two types of hydrogels based on methacryloyl-modified gelatin (GM) as three-dimensional scaffolds was examined for its support of tissue specific cell functions.
29981277	3	65	theme	supporting	721:730	arg1	cells					732:736	supporting cells	721:736	supporting cells	721:736	HDMECs, together with hASCs as supporting cells, were encapsulated in soft GM gels and were indirectly cocultured with hASCs encapsulated in stiffer GM hydrogels additionally containing methacrylate-modified hyaluronic acid and hydroxyapatite particles.
29981277	8	66	theme	cell	1723:1726	arg1	types					1728:1732	cocultured cell types	1712:1732	cocultured cell types	1712:1732	We discussed and concluded that osteogenic and angiogenic signals from the culture media as well as from cocultured cell types, and tissue-specific hydrogel composition all contribute to stimulate the interplay between osteogenesis and angiogenesis in vitro and are a basis for engineering vascularized bone.
29981277	8	67	from	types	1728:1732	arg1	signals					1665:1671	osteogenic and angiogenic signals	1639:1671	osteogenic and angiogenic signals from the culture media as well as from cocultured cell types,	1639:1733	We discussed and concluded that osteogenic and angiogenic signals from the culture media as well as from cocultured cell types, and tissue-specific hydrogel composition all contribute to stimulate the interplay between osteogenesis and angiogenesis in vitro and are a basis for engineering vascularized bone.
29981277	8	67	from	types	1728:1732	arg1	composition					1764:1774	tissue-specific hydrogel composition	1739:1774	tissue-specific hydrogel composition	1739:1774	We discussed and concluded that osteogenic and angiogenic signals from the culture media as well as from cocultured cell types, and tissue-specific hydrogel composition all contribute to stimulate the interplay between osteogenesis and angiogenesis in vitro and are a basis for engineering vascularized bone.
29981277	4	68	theme	bone-specific	1094:1106	arg1	osteopontin					1117:1127	osteopontin	1117:1127	osteopontin	1117:1127	After 14 days, the hASC in the stiffer gels (constituting the "bone gels") expressed matrix proteins like collagen type I and fibronectin, as well as bone-specific proteins osteopontin and alkaline phosphatase.
29981277	4	68	theme	bone-specific	1094:1106	arg1	phosphatase					1142:1152	alkaline phosphatase	1133:1152	alkaline phosphatase	1133:1152	After 14 days, the hASC in the stiffer gels (constituting the "bone gels") expressed matrix proteins like collagen type I and fibronectin, as well as bone-specific proteins osteopontin and alkaline phosphatase.
29981277	4	68	theme	bone-specific	1094:1106	arg1	proteins					1108:1115	bone-specific proteins	1094:1115	bone-specific proteins osteopontin and alkaline phosphatase	1094:1152	After 14 days, the hASC in the stiffer gels (constituting the "bone gels") expressed matrix proteins like collagen type I and fibronectin, as well as bone-specific proteins osteopontin and alkaline phosphatase.
29981277	5	69	theme	HDMEC-laden	1187:1197	arg1	hydrogels					1199:1207	HDMEC-laden hydrogels	1187:1207	HDMEC-laden hydrogels	1187:1207	After 14 days of coculture with HDMEC-laden hydrogels, the viscoelastic properties of the bone gels were significantly higher compared with the gels in monoculture.
29981277	6	70	theme	soft	1331:1334	arg1	gels					1352:1355	the soft vascularization gels	1327:1355	the soft vascularization gels	1327:1355	Within the soft vascularization gels, the formed capillary-like networks were significantly longer after 14 days of coculture than the structures in the control gels.
29981277	3	71	theme	stiffer	831:837	arg1	hydrogels					842:850	stiffer GM hydrogels	831:850	stiffer GM hydrogels additionally containing methacrylate-modified hyaluronic acid and hydroxyapatite particles	831:941	HDMECs, together with hASCs as supporting cells, were encapsulated in soft GM gels and were indirectly cocultured with hASCs encapsulated in stiffer GM hydrogels additionally containing methacrylate-modified hyaluronic acid and hydroxyapatite particles.
29981277	8	72	theme	culture	1682:1688	arg1	media					1690:1694	the culture media	1678:1694	the culture media	1678:1694	We discussed and concluded that osteogenic and angiogenic signals from the culture media as well as from cocultured cell types, and tissue-specific hydrogel composition all contribute to stimulate the interplay between osteogenesis and angiogenesis in vitro and are a basis for engineering vascularized bone.
29981277	5	73	theme	gels	1250:1253	arg1	properties					1227:1236	the viscoelastic properties	1210:1236	the viscoelastic properties of the bone gels	1210:1253	After 14 days of coculture with HDMEC-laden hydrogels, the viscoelastic properties of the bone gels were significantly higher compared with the gels in monoculture.
29981277	5	73	theme	gels	1250:1253	arg1	higher					1274:1279	higher	1274:1279	higher	1274:1279	After 14 days of coculture with HDMEC-laden hydrogels, the viscoelastic properties of the bone gels were significantly higher compared with the gels in monoculture.
29981277	0	74	from	cells	98:102	arg1	hydrogels					150:158	tissue-specific methacryloyl gelatin-based hydrogels	107:158	tissue-specific methacryloyl gelatin-based hydrogels	107:158	Improved vasculogenesis and bone matrix formation through coculture of endothelial cells and stem cells in tissue-specific methacryloyl gelatin-based hydrogels.
29981277	3	75	theme	methacrylate-modified	876:896	arg1	acid					909:912	methacrylate-modified hyaluronic acid	876:912	methacrylate-modified hyaluronic acid	876:912	HDMECs, together with hASCs as supporting cells, were encapsulated in soft GM gels and were indirectly cocultured with hASCs encapsulated in stiffer GM hydrogels additionally containing methacrylate-modified hyaluronic acid and hydroxyapatite particles.
29981277	0	76	from	hydrogels	150:158	arg1	coculture					58:66	coculture	58:66	coculture of endothelial cells and stem cells in tissue-specific methacryloyl gelatin-based hydrogels	58:158	Improved vasculogenesis and bone matrix formation through coculture of endothelial cells and stem cells in tissue-specific methacryloyl gelatin-based hydrogels.
29981277	0	77	theme	endothelial	71:81	arg1	cells					83:87	endothelial cells	71:87	endothelial cells	71:87	Improved vasculogenesis and bone matrix formation through coculture of endothelial cells and stem cells in tissue-specific methacryloyl gelatin-based hydrogels.
29981277	4	78	from	hASC	963:966	arg1	gels					983:986	the stiffer gels	971:986	the stiffer gels	971:986	After 14 days, the hASC in the stiffer gels (constituting the "bone gels") expressed matrix proteins like collagen type I and fibronectin, as well as bone-specific proteins osteopontin and alkaline phosphatase.
29981277	2	79	theme	adipose-derived	420:434	arg1	hASCs					448:452	hASCs	448:452	hASCs	448:452	In this study, a coculture system combining primary human adipose-derived stem cells (hASCs) and primary human dermal microvascular endothelial cells (HDMECs) within two types of hydrogels based on methacryloyl-modified gelatin (GM) as three-dimensional scaffolds was examined for its support of tissue specific cell functions.
29981277	2	79	theme	adipose-derived	420:434	arg1	cells					441:445	primary human adipose-derived stem cells	406:445	primary human adipose-derived stem cells (hASCs)	406:453	In this study, a coculture system combining primary human adipose-derived stem cells (hASCs) and primary human dermal microvascular endothelial cells (HDMECs) within two types of hydrogels based on methacryloyl-modified gelatin (GM) as three-dimensional scaffolds was examined for its support of tissue specific cell functions.
29981277	3	80	dep	together	698:705	arg1	with					707:710	with	707:710	with	707:710	HDMECs, together with hASCs as supporting cells, were encapsulated in soft GM gels and were indirectly cocultured with hASCs encapsulated in stiffer GM hydrogels additionally containing methacrylate-modified hyaluronic acid and hydroxyapatite particles.
29981277	1	81	dep	engineering	312:322	arg1	vascularized					324:335	vascularized	324:335	vascularized	324:335	The coculture of osteogenic and angiogenic cells and the resulting paracrine signaling via soluble factors are supposed to be crucial for successfully engineering vascularized bone tissue equivalents.
29981277	8	82	theme	osteogenic	1639:1648	arg1	signals					1665:1671	osteogenic and angiogenic signals	1639:1671	osteogenic and angiogenic signals from the culture media as well as from cocultured cell types,	1639:1733	We discussed and concluded that osteogenic and angiogenic signals from the culture media as well as from cocultured cell types, and tissue-specific hydrogel composition all contribute to stimulate the interplay between osteogenesis and angiogenesis in vitro and are a basis for engineering vascularized bone.
29981277	4	83	theme	collagen	1050:1057	arg1	type					1059:1062	collagen type I	1050:1064	collagen type I	1050:1064	After 14 days, the hASC in the stiffer gels (constituting the "bone gels") expressed matrix proteins like collagen type I and fibronectin, as well as bone-specific proteins osteopontin and alkaline phosphatase.
29981277	2	84	theme	hydrogels	541:549	arg1	types					532:536	two types	528:536	two types of hydrogels based on methacryloyl-modified gelatin (GM) as three-dimensional scaffolds	528:624	In this study, a coculture system combining primary human adipose-derived stem cells (hASCs) and primary human dermal microvascular endothelial cells (HDMECs) within two types of hydrogels based on methacryloyl-modified gelatin (GM) as three-dimensional scaffolds was examined for its support of tissue specific cell functions.
31145641	8	0	dep	treating	1569:1576	arg1	ameliorates					1676:1686	ameliorates	1676:1686	treating IBD.-Ji, J., Ge, X., Chen, Y., Zhu, B., Wu, Q., Zhang, J., Shan, J., Cheng, H., Shi, L. Daphnetin ameliorates experimental colitis by modulating microbiota composition and Treg/Th17 balance	1569:1766	We thus for the first time reveal the potential of DAPH in resetting the gut microbiome and reestablishing immune homeostasis in colitic mice, which may have clinical implications for treating IBD.-Ji, J., Ge, X., Chen, Y., Zhu, B., Wu, Q., Zhang, J., Shan, J., Cheng, H., Shi, L. Daphnetin ameliorates experimental colitis by modulating microbiota composition and Treg/Th17 balance.
31145641	7	1	theme	SCFA-producing	1339:1352	arg1	microbiota					1358:1367	SCFA-producing gut microbiota	1339:1367	SCFA-producing gut microbiota	1339:1367	More critically, the protective effect of DAPH was shown to be transmissible among colitic mice through cohousing or fecal microbiota transplantation, further substantiating the importance of SCFA-producing gut microbiota in DAPH action.
31145641	5	2	theme	microbiota	816:825	arg1	composition					797:807	the composition	793:807	the composition of gut microbiota	793:825	Our analysis showed that DAPH modified the composition of gut microbiota and altered the metabolic profiles in dextran sulfate sodium-treated mice.
31145641	6	3	theme	cells	1082:1086	arg1	differentiation					1130:1144	the reduced proinflammatory Th17 cell differentiation	1092:1144	the reduced proinflammatory Th17 cell differentiation	1092:1144	In particular, this agent significantly elevated the abundance of short-chain fatty acid (SCFA)-producing gut microbiota, causatively related with the enhanced development of Treg cells and the reduced proinflammatory Th17 cell differentiation.
31145641	6	3	theme	cells	1082:1086	arg1	development					1062:1072	the enhanced development	1049:1072	the enhanced development of Treg cells	1049:1086	In particular, this agent significantly elevated the abundance of short-chain fatty acid (SCFA)-producing gut microbiota, causatively related with the enhanced development of Treg cells and the reduced proinflammatory Th17 cell differentiation.
31145641	5	4	theme	metabolic	843:851	arg1	profiles					853:860	the metabolic profiles	839:860	the metabolic profiles in dextran sulfate sodium-treated mice	839:899	Our analysis showed that DAPH modified the composition of gut microbiota and altered the metabolic profiles in dextran sulfate sodium-treated mice.
31145641	5	5	from	profiles	853:860	arg1	mice					896:899	dextran sulfate sodium-treated mice	865:899	dextran sulfate sodium-treated mice	865:899	Our analysis showed that DAPH modified the composition of gut microbiota and altered the metabolic profiles in dextran sulfate sodium-treated mice.
31145641	7	6	theme	gut	1354:1356	arg1	microbiota					1358:1367	SCFA-producing gut microbiota	1339:1367	SCFA-producing gut microbiota	1339:1367	More critically, the protective effect of DAPH was shown to be transmissible among colitic mice through cohousing or fecal microbiota transplantation, further substantiating the importance of SCFA-producing gut microbiota in DAPH action.
31145641	6	7	theme	Treg	1077:1080	arg1	cells					1082:1086	Treg cells	1077:1086	Treg cells	1077:1086	In particular, this agent significantly elevated the abundance of short-chain fatty acid (SCFA)-producing gut microbiota, causatively related with the enhanced development of Treg cells and the reduced proinflammatory Th17 cell differentiation.
31145641	8	8	theme	colitic	1514:1520	arg1	mice					1522:1525	colitic mice	1514:1525	colitic mice	1514:1525	We thus for the first time reveal the potential of DAPH in resetting the gut microbiome and reestablishing immune homeostasis in colitic mice, which may have clinical implications for treating IBD.-Ji, J., Ge, X., Chen, Y., Zhu, B., Wu, Q., Zhang, J., Shan, J., Cheng, H., Shi, L. Daphnetin ameliorates experimental colitis by modulating microbiota composition and Treg/Th17 balance.
31145641	8	9	theme	DAPH	1436:1439	arg1	potential					1423:1431	the potential	1419:1431	the potential of DAPH	1419:1439	We thus for the first time reveal the potential of DAPH in resetting the gut microbiome and reestablishing immune homeostasis in colitic mice, which may have clinical implications for treating IBD.-Ji, J., Ge, X., Chen, Y., Zhu, B., Wu, Q., Zhang, J., Shan, J., Cheng, H., Shi, L. Daphnetin ameliorates experimental colitis by modulating microbiota composition and Treg/Th17 balance.
31145641	8	10	contain	have	1538:1541	arg2	implications					1552:1563	clinical implications	1543:1563	clinical implications for treating IBD.-Ji, J., Ge, X., Chen, Y., Zhu, B., Wu, Q., Zhang, J., Shan, J., Cheng, H., Shi, L. Daphnetin ameliorates experimental colitis by modulating microbiota composition and Treg/Th17 balance	1543:1766	We thus for the first time reveal the potential of DAPH in resetting the gut microbiome and reestablishing immune homeostasis in colitic mice, which may have clinical implications for treating IBD.-Ji, J., Ge, X., Chen, Y., Zhu, B., Wu, Q., Zhang, J., Shan, J., Cheng, H., Shi, L. Daphnetin ameliorates experimental colitis by modulating microbiota composition and Treg/Th17 balance.
31145641	8	10	contain	have	1538:1541	arg1	which					1528:1532	which	1528:1532	which	1528:1532	We thus for the first time reveal the potential of DAPH in resetting the gut microbiome and reestablishing immune homeostasis in colitic mice, which may have clinical implications for treating IBD.-Ji, J., Ge, X., Chen, Y., Zhu, B., Wu, Q., Zhang, J., Shan, J., Cheng, H., Shi, L. Daphnetin ameliorates experimental colitis by modulating microbiota composition and Treg/Th17 balance.
31145641	4	11	theme	colonic	654:660	arg1	integrity					662:670	colonic integrity	654:670	colonic integrity	654:670	Herein, we showed that DAPH remarkably alleviated experimental colitis by reducing colonic inflammation, improving colonic integrity, and reestablishing immune and metabolic homeostasis in the inflicted intestines.
31145641	1	12	theme	Inflammatory	103:114	arg1	IBDs					132:135	IBDs	132:135	IBDs	132:135	Inflammatory bowel diseases (IBDs) are characterized by chronic pathologies associated with extensive gut dysbiosis and intestinal inflammation.
31145641	1	12	theme	Inflammatory	103:114	arg1	diseases					122:129	Inflammatory bowel diseases	103:129	Inflammatory bowel diseases (IBDs)	103:136	Inflammatory bowel diseases (IBDs) are characterized by chronic pathologies associated with extensive gut dysbiosis and intestinal inflammation.
31145641	6	13	theme	acid	986:989	arg1	abundance					955:963	the abundance	951:963	the abundance of short-chain fatty acid (SCFA)-producing gut microbiota, causatively related with the enhanced development of Treg cells and the reduced proinflammatory Th17 cell differentiation	951:1144	In particular, this agent significantly elevated the abundance of short-chain fatty acid (SCFA)-producing gut microbiota, causatively related with the enhanced development of Treg cells and the reduced proinflammatory Th17 cell differentiation.
31145641	2	14	theme	gut	319:321	arg1	microbiota					323:332	gut microbiota	319:332	gut microbiota	319:332	Hence, endeavors to improve the inflammatory pathology by manipulating gut microbiota are ongoing.
31145641	5	15	theme	gut	812:814	arg1	microbiota					816:825	gut microbiota	812:825	gut microbiota	812:825	Our analysis showed that DAPH modified the composition of gut microbiota and altered the metabolic profiles in dextran sulfate sodium-treated mice.
31145641	6	16	theme	fatty	980:984	arg1	SCFA					992:995	SCFA	992:995	SCFA	992:995	In particular, this agent significantly elevated the abundance of short-chain fatty acid (SCFA)-producing gut microbiota, causatively related with the enhanced development of Treg cells and the reduced proinflammatory Th17 cell differentiation.
31145641	6	16	theme	fatty	980:984	arg1	acid					986:989	short-chain fatty acid	968:989	short-chain fatty acid (SCFA)-producing gut microbiota, causatively related with the enhanced development of Treg cells and the reduced proinflammatory Th17 cell differentiation	968:1144	In particular, this agent significantly elevated the abundance of short-chain fatty acid (SCFA)-producing gut microbiota, causatively related with the enhanced development of Treg cells and the reduced proinflammatory Th17 cell differentiation.
31145641	4	17	theme	immune	692:697	arg1	homeostasis					713:723	immune and metabolic homeostasis	692:723	homeostasis	713:723	Herein, we showed that DAPH remarkably alleviated experimental colitis by reducing colonic inflammation, improving colonic integrity, and reestablishing immune and metabolic homeostasis in the inflicted intestines.
31145641	8	18	from	homeostasis	1499:1509	arg1	mice					1522:1525	colitic mice	1514:1525	colitic mice	1514:1525	We thus for the first time reveal the potential of DAPH in resetting the gut microbiome and reestablishing immune homeostasis in colitic mice, which may have clinical implications for treating IBD.-Ji, J., Ge, X., Chen, Y., Zhu, B., Wu, Q., Zhang, J., Shan, J., Cheng, H., Shi, L. Daphnetin ameliorates experimental colitis by modulating microbiota composition and Treg/Th17 balance.
31145641	3	19	theme	coumarin	369:376	arg1	derivative					378:387	a coumarin derivative	367:387	a coumarin derivative extracted from Daphne odora var with anti-inflammatory and immune-regulatory properties that has been widely used in treating inflammatory disorders	367:536	Daphnetin (DAPH) is a coumarin derivative extracted from Daphne odora var with anti-inflammatory and immune-regulatory properties that has been widely used in treating inflammatory disorders.
31145641	3	19	theme	coumarin	369:376	arg1	Daphnetin					347:355	Daphnetin	347:355	Daphnetin (DAPH)	347:362	Daphnetin (DAPH) is a coumarin derivative extracted from Daphne odora var with anti-inflammatory and immune-regulatory properties that has been widely used in treating inflammatory disorders.
31145641	6	20	theme	short-chain	968:978	arg1	SCFA					992:995	SCFA	992:995	SCFA	992:995	In particular, this agent significantly elevated the abundance of short-chain fatty acid (SCFA)-producing gut microbiota, causatively related with the enhanced development of Treg cells and the reduced proinflammatory Th17 cell differentiation.
31145641	6	20	theme	short-chain	968:978	arg1	acid					986:989	short-chain fatty acid	968:989	short-chain fatty acid (SCFA)-producing gut microbiota, causatively related with the enhanced development of Treg cells and the reduced proinflammatory Th17 cell differentiation	968:1144	In particular, this agent significantly elevated the abundance of short-chain fatty acid (SCFA)-producing gut microbiota, causatively related with the enhanced development of Treg cells and the reduced proinflammatory Th17 cell differentiation.
31145641	5	21	theme	sodium-treated	881:894	arg1	mice					896:899	dextran sulfate sodium-treated mice	865:899	dextran sulfate sodium-treated mice	865:899	Our analysis showed that DAPH modified the composition of gut microbiota and altered the metabolic profiles in dextran sulfate sodium-treated mice.
31145641	8	22	theme	experimental	1688:1699	arg1	colitis					1701:1707	experimental colitis	1688:1707	experimental colitis by modulating microbiota composition and Treg/Th17 balance	1688:1766	We thus for the first time reveal the potential of DAPH in resetting the gut microbiome and reestablishing immune homeostasis in colitic mice, which may have clinical implications for treating IBD.-Ji, J., Ge, X., Chen, Y., Zhu, B., Wu, Q., Zhang, J., Shan, J., Cheng, H., Shi, L. Daphnetin ameliorates experimental colitis by modulating microbiota composition and Treg/Th17 balance.
31145641	1	23	theme	extensive	195:203	arg1	dysbiosis					209:217	extensive gut dysbiosis	195:217	extensive gut dysbiosis	195:217	Inflammatory bowel diseases (IBDs) are characterized by chronic pathologies associated with extensive gut dysbiosis and intestinal inflammation.
31145641	3	24	theme	odora	411:415	arg1	var					417:419	Daphne odora var	404:419	Daphne odora var with anti-inflammatory and immune-regulatory properties that has been widely used in treating inflammatory disorders	404:536	Daphnetin (DAPH) is a coumarin derivative extracted from Daphne odora var with anti-inflammatory and immune-regulatory properties that has been widely used in treating inflammatory disorders.
31145641	4	25	from	homeostasis	713:723	arg1	intestines					742:751	the inflicted intestines	728:751	the inflicted intestines	728:751	Herein, we showed that DAPH remarkably alleviated experimental colitis by reducing colonic inflammation, improving colonic integrity, and reestablishing immune and metabolic homeostasis in the inflicted intestines.
31145641	7	26	theme	protective	1168:1177	arg1	transmissible					1210:1222	transmissible	1210:1222	transmissible	1210:1222	More critically, the protective effect of DAPH was shown to be transmissible among colitic mice through cohousing or fecal microbiota transplantation, further substantiating the importance of SCFA-producing gut microbiota in DAPH action.
31145641	7	26	theme	protective	1168:1177	arg1	effect					1179:1184	the protective effect	1164:1184	the protective effect of DAPH	1164:1192	More critically, the protective effect of DAPH was shown to be transmissible among colitic mice through cohousing or fecal microbiota transplantation, further substantiating the importance of SCFA-producing gut microbiota in DAPH action.
31145641	3	27	used	used	498:501	arg2	properties					466:475	anti-inflammatory and immune-regulatory properties	426:475	anti-inflammatory and immune-regulatory properties that has been widely used in treating inflammatory disorders	426:536	Daphnetin (DAPH) is a coumarin derivative extracted from Daphne odora var with anti-inflammatory and immune-regulatory properties that has been widely used in treating inflammatory disorders.
31145641	1	28	theme	gut	205:207	arg1	dysbiosis					209:217	extensive gut dysbiosis	195:217	extensive gut dysbiosis	195:217	Inflammatory bowel diseases (IBDs) are characterized by chronic pathologies associated with extensive gut dysbiosis and intestinal inflammation.
31145641	7	29	theme	fecal	1264:1268	arg1	transplantation					1281:1295	fecal microbiota transplantation	1264:1295	fecal microbiota transplantation	1264:1295	More critically, the protective effect of DAPH was shown to be transmissible among colitic mice through cohousing or fecal microbiota transplantation, further substantiating the importance of SCFA-producing gut microbiota in DAPH action.
31145641	6	30	theme	enhanced	1053:1060	arg1	development					1062:1072	the enhanced development	1049:1072	the enhanced development of Treg cells	1049:1086	In particular, this agent significantly elevated the abundance of short-chain fatty acid (SCFA)-producing gut microbiota, causatively related with the enhanced development of Treg cells and the reduced proinflammatory Th17 cell differentiation.
31145641	8	31	dep	Daphnetin	1666:1674	arg1	J.					1587:1588	J.	1587:1588	J.	1587:1588	We thus for the first time reveal the potential of DAPH in resetting the gut microbiome and reestablishing immune homeostasis in colitic mice, which may have clinical implications for treating IBD.-Ji, J., Ge, X., Chen, Y., Zhu, B., Wu, Q., Zhang, J., Shan, J., Cheng, H., Shi, L. Daphnetin ameliorates experimental colitis by modulating microbiota composition and Treg/Th17 balance.
31145641	1	32	theme	bowel	116:120	arg1	IBDs					132:135	IBDs	132:135	IBDs	132:135	Inflammatory bowel diseases (IBDs) are characterized by chronic pathologies associated with extensive gut dysbiosis and intestinal inflammation.
31145641	1	32	theme	bowel	116:120	arg1	diseases					122:129	Inflammatory bowel diseases	103:129	Inflammatory bowel diseases (IBDs)	103:136	Inflammatory bowel diseases (IBDs) are characterized by chronic pathologies associated with extensive gut dysbiosis and intestinal inflammation.
31145641	0	33	theme	microbiota	57:66	arg1	composition					68:78	microbiota composition	57:78	microbiota composition	57:78	Daphnetin ameliorates experimental colitis by modulating microbiota composition and Treg/Th17 balance.
31145641	4	34	theme	inflicted	732:740	arg1	intestines					742:751	the inflicted intestines	728:751	the inflicted intestines	728:751	Herein, we showed that DAPH remarkably alleviated experimental colitis by reducing colonic inflammation, improving colonic integrity, and reestablishing immune and metabolic homeostasis in the inflicted intestines.
31145641	8	35	theme	first	1401:1405	arg1	time					1407:1410	the first time	1397:1410	the first time	1397:1410	We thus for the first time reveal the potential of DAPH in resetting the gut microbiome and reestablishing immune homeostasis in colitic mice, which may have clinical implications for treating IBD.-Ji, J., Ge, X., Chen, Y., Zhu, B., Wu, Q., Zhang, J., Shan, J., Cheng, H., Shi, L. Daphnetin ameliorates experimental colitis by modulating microbiota composition and Treg/Th17 balance.
31145641	7	36	theme	DAPH	1189:1192	arg1	transmissible					1210:1222	transmissible	1210:1222	transmissible	1210:1222	More critically, the protective effect of DAPH was shown to be transmissible among colitic mice through cohousing or fecal microbiota transplantation, further substantiating the importance of SCFA-producing gut microbiota in DAPH action.
31145641	7	36	theme	DAPH	1189:1192	arg1	effect					1179:1184	the protective effect	1164:1184	the protective effect of DAPH	1164:1192	More critically, the protective effect of DAPH was shown to be transmissible among colitic mice through cohousing or fecal microbiota transplantation, further substantiating the importance of SCFA-producing gut microbiota in DAPH action.
31145641	3	37	theme	Daphne	404:409	arg1	var					417:419	Daphne odora var	404:419	Daphne odora var with anti-inflammatory and immune-regulatory properties that has been widely used in treating inflammatory disorders	404:536	Daphnetin (DAPH) is a coumarin derivative extracted from Daphne odora var with anti-inflammatory and immune-regulatory properties that has been widely used in treating inflammatory disorders.
31145641	4	38	theme	colonic	622:628	arg1	inflammation					630:641	colonic inflammation	622:641	colonic inflammation	622:641	Herein, we showed that DAPH remarkably alleviated experimental colitis by reducing colonic inflammation, improving colonic integrity, and reestablishing immune and metabolic homeostasis in the inflicted intestines.
31145641	3	39	with	var	417:419	arg1	properties					466:475	anti-inflammatory and immune-regulatory properties	426:475	anti-inflammatory and immune-regulatory properties that has been widely used in treating inflammatory disorders	426:536	Daphnetin (DAPH) is a coumarin derivative extracted from Daphne odora var with anti-inflammatory and immune-regulatory properties that has been widely used in treating inflammatory disorders.
31145641	8	40	theme	gut	1458:1460	arg1	microbiome					1462:1471	the gut microbiome	1454:1471	the gut microbiome	1454:1471	We thus for the first time reveal the potential of DAPH in resetting the gut microbiome and reestablishing immune homeostasis in colitic mice, which may have clinical implications for treating IBD.-Ji, J., Ge, X., Chen, Y., Zhu, B., Wu, Q., Zhang, J., Shan, J., Cheng, H., Shi, L. Daphnetin ameliorates experimental colitis by modulating microbiota composition and Treg/Th17 balance.
31145641	1	41	theme	intestinal	223:232	arg1	inflammation					234:245	intestinal inflammation	223:245	intestinal inflammation	223:245	Inflammatory bowel diseases (IBDs) are characterized by chronic pathologies associated with extensive gut dysbiosis and intestinal inflammation.
31145641	7	42	theme	colitic	1230:1236	arg1	mice					1238:1241	colitic mice	1230:1241	colitic mice	1230:1241	More critically, the protective effect of DAPH was shown to be transmissible among colitic mice through cohousing or fecal microbiota transplantation, further substantiating the importance of SCFA-producing gut microbiota in DAPH action.
31145641	8	43	theme	immune	1492:1497	arg1	homeostasis					1499:1509	immune homeostasis	1492:1509	immune homeostasis in colitic mice	1492:1525	We thus for the first time reveal the potential of DAPH in resetting the gut microbiome and reestablishing immune homeostasis in colitic mice, which may have clinical implications for treating IBD.-Ji, J., Ge, X., Chen, Y., Zhu, B., Wu, Q., Zhang, J., Shan, J., Cheng, H., Shi, L. Daphnetin ameliorates experimental colitis by modulating microbiota composition and Treg/Th17 balance.
31145641	6	44	theme	cell	1125:1128	arg1	differentiation					1130:1144	the reduced proinflammatory Th17 cell differentiation	1092:1144	the reduced proinflammatory Th17 cell differentiation	1092:1144	In particular, this agent significantly elevated the abundance of short-chain fatty acid (SCFA)-producing gut microbiota, causatively related with the enhanced development of Treg cells and the reduced proinflammatory Th17 cell differentiation.
31145641	3	45	theme	immune-regulatory	448:464	arg1	properties					466:475	anti-inflammatory and immune-regulatory properties	426:475	anti-inflammatory and immune-regulatory properties that has been widely used in treating inflammatory disorders	426:536	Daphnetin (DAPH) is a coumarin derivative extracted from Daphne odora var with anti-inflammatory and immune-regulatory properties that has been widely used in treating inflammatory disorders.
31145641	2	46	dep	endeavors	255:263	arg1	improve					268:274	improve	268:274	to improve the inflammatory pathology by manipulating gut microbiota	265:332	Hence, endeavors to improve the inflammatory pathology by manipulating gut microbiota are ongoing.
31145641	5	47	theme	dextran	865:871	arg1	sulfate					873:879	dextran sulfate	865:879	dextran sulfate sodium-treated mice	865:899	Our analysis showed that DAPH modified the composition of gut microbiota and altered the metabolic profiles in dextran sulfate sodium-treated mice.
31145641	5	48	theme	sulfate	873:879	arg1	mice					896:899	dextran sulfate sodium-treated mice	865:899	dextran sulfate sodium-treated mice	865:899	Our analysis showed that DAPH modified the composition of gut microbiota and altered the metabolic profiles in dextran sulfate sodium-treated mice.
31145641	8	49	theme	clinical	1543:1550	arg1	implications					1552:1563	clinical implications	1543:1563	clinical implications for treating IBD.-Ji, J., Ge, X., Chen, Y., Zhu, B., Wu, Q., Zhang, J., Shan, J., Cheng, H., Shi, L. Daphnetin ameliorates experimental colitis by modulating microbiota composition and Treg/Th17 balance	1543:1766	We thus for the first time reveal the potential of DAPH in resetting the gut microbiome and reestablishing immune homeostasis in colitic mice, which may have clinical implications for treating IBD.-Ji, J., Ge, X., Chen, Y., Zhu, B., Wu, Q., Zhang, J., Shan, J., Cheng, H., Shi, L. Daphnetin ameliorates experimental colitis by modulating microbiota composition and Treg/Th17 balance.
31145641	8	50	dep	reveal	1412:1417	arg1	have					1538:1541	have	1538:1541	may have clinical implications for treating IBD.-Ji, J., Ge, X., Chen, Y., Zhu, B., Wu, Q., Zhang, J., Shan, J., Cheng, H., Shi, L. Daphnetin ameliorates experimental colitis by modulating microbiota composition and Treg/Th17 balance	1534:1766	We thus for the first time reveal the potential of DAPH in resetting the gut microbiome and reestablishing immune homeostasis in colitic mice, which may have clinical implications for treating IBD.-Ji, J., Ge, X., Chen, Y., Zhu, B., Wu, Q., Zhang, J., Shan, J., Cheng, H., Shi, L. Daphnetin ameliorates experimental colitis by modulating microbiota composition and Treg/Th17 balance.
31145641	3	51	theme	anti-inflammatory	426:442	arg1	properties					466:475	anti-inflammatory and immune-regulatory properties	426:475	anti-inflammatory and immune-regulatory properties that has been widely used in treating inflammatory disorders	426:536	Daphnetin (DAPH) is a coumarin derivative extracted from Daphne odora var with anti-inflammatory and immune-regulatory properties that has been widely used in treating inflammatory disorders.
31145641	4	52	theme	experimental	589:600	arg1	colitis					602:608	experimental colitis	589:608	experimental colitis	589:608	Herein, we showed that DAPH remarkably alleviated experimental colitis by reducing colonic inflammation, improving colonic integrity, and reestablishing immune and metabolic homeostasis in the inflicted intestines.
31145641	6	53	theme	Th17	1120:1123	arg1	differentiation					1130:1144	the reduced proinflammatory Th17 cell differentiation	1092:1144	the reduced proinflammatory Th17 cell differentiation	1092:1144	In particular, this agent significantly elevated the abundance of short-chain fatty acid (SCFA)-producing gut microbiota, causatively related with the enhanced development of Treg cells and the reduced proinflammatory Th17 cell differentiation.
31145641	6	54	theme	gut	1008:1010	arg1	microbiota					1012:1021	gut microbiota	1008:1021	gut microbiota	1008:1021	In particular, this agent significantly elevated the abundance of short-chain fatty acid (SCFA)-producing gut microbiota, causatively related with the enhanced development of Treg cells and the reduced proinflammatory Th17 cell differentiation.
31145641	8	55	theme	microbiota	1723:1732	arg1	composition					1734:1744	microbiota composition	1723:1744	microbiota composition	1723:1744	We thus for the first time reveal the potential of DAPH in resetting the gut microbiome and reestablishing immune homeostasis in colitic mice, which may have clinical implications for treating IBD.-Ji, J., Ge, X., Chen, Y., Zhu, B., Wu, Q., Zhang, J., Shan, J., Cheng, H., Shi, L. Daphnetin ameliorates experimental colitis by modulating microbiota composition and Treg/Th17 balance.
31145641	8	56	theme	Treg/Th17	1750:1758	arg1	balance					1760:1766	Treg/Th17 balance	1750:1766	Treg/Th17 balance	1750:1766	We thus for the first time reveal the potential of DAPH in resetting the gut microbiome and reestablishing immune homeostasis in colitic mice, which may have clinical implications for treating IBD.-Ji, J., Ge, X., Chen, Y., Zhu, B., Wu, Q., Zhang, J., Shan, J., Cheng, H., Shi, L. Daphnetin ameliorates experimental colitis by modulating microbiota composition and Treg/Th17 balance.
31145641	3	57	theme	inflammatory	515:526	arg1	disorders					528:536	inflammatory disorders	515:536	inflammatory disorders	515:536	Daphnetin (DAPH) is a coumarin derivative extracted from Daphne odora var with anti-inflammatory and immune-regulatory properties that has been widely used in treating inflammatory disorders.
31145641	6	58	theme	proinflammatory	1104:1118	arg1	differentiation					1130:1144	the reduced proinflammatory Th17 cell differentiation	1092:1144	the reduced proinflammatory Th17 cell differentiation	1092:1144	In particular, this agent significantly elevated the abundance of short-chain fatty acid (SCFA)-producing gut microbiota, causatively related with the enhanced development of Treg cells and the reduced proinflammatory Th17 cell differentiation.
31145641	7	59	from	importance	1325:1334	arg1	action					1377:1382	DAPH action	1372:1382	DAPH action	1372:1382	More critically, the protective effect of DAPH was shown to be transmissible among colitic mice through cohousing or fecal microbiota transplantation, further substantiating the importance of SCFA-producing gut microbiota in DAPH action.
31145641	2	60	theme	inflammatory	280:291	arg1	pathology					293:301	the inflammatory pathology	276:301	the inflammatory pathology	276:301	Hence, endeavors to improve the inflammatory pathology by manipulating gut microbiota are ongoing.
31145641	6	61	theme	reduced	1096:1102	arg1	differentiation					1130:1144	the reduced proinflammatory Th17 cell differentiation	1092:1144	the reduced proinflammatory Th17 cell differentiation	1092:1144	In particular, this agent significantly elevated the abundance of short-chain fatty acid (SCFA)-producing gut microbiota, causatively related with the enhanced development of Treg cells and the reduced proinflammatory Th17 cell differentiation.
31145641	0	62	theme	Treg/Th17	84:92	arg1	balance					94:100	Treg/Th17 balance	84:100	Treg/Th17 balance	84:100	Daphnetin ameliorates experimental colitis by modulating microbiota composition and Treg/Th17 balance.
31145641	4	63	theme	metabolic	703:711	arg1	homeostasis					713:723	immune and metabolic homeostasis	692:723	homeostasis	713:723	Herein, we showed that DAPH remarkably alleviated experimental colitis by reducing colonic inflammation, improving colonic integrity, and reestablishing immune and metabolic homeostasis in the inflicted intestines.
31145641	7	64	theme	microbiota	1358:1367	arg1	importance					1325:1334	the importance	1321:1334	the importance of SCFA-producing gut microbiota in DAPH action	1321:1382	More critically, the protective effect of DAPH was shown to be transmissible among colitic mice through cohousing or fecal microbiota transplantation, further substantiating the importance of SCFA-producing gut microbiota in DAPH action.
31145641	7	65	theme	microbiota	1270:1279	arg1	transplantation					1281:1295	fecal microbiota transplantation	1264:1295	fecal microbiota transplantation	1264:1295	More critically, the protective effect of DAPH was shown to be transmissible among colitic mice through cohousing or fecal microbiota transplantation, further substantiating the importance of SCFA-producing gut microbiota in DAPH action.
31145641	0	66	theme	experimental	22:33	arg1	colitis					35:41	experimental colitis	22:41	experimental colitis	22:41	Daphnetin ameliorates experimental colitis by modulating microbiota composition and Treg/Th17 balance.
31145641	7	67	theme	DAPH	1372:1375	arg1	action					1377:1382	DAPH action	1372:1382	DAPH action	1372:1382	More critically, the protective effect of DAPH was shown to be transmissible among colitic mice through cohousing or fecal microbiota transplantation, further substantiating the importance of SCFA-producing gut microbiota in DAPH action.
31145641	1	68	theme	chronic	159:165	arg1	pathologies					167:177	chronic pathologies	159:177	chronic pathologies associated with extensive gut dysbiosis and intestinal inflammation	159:245	Inflammatory bowel diseases (IBDs) are characterized by chronic pathologies associated with extensive gut dysbiosis and intestinal inflammation.
30660720	5	0	theme	normal	1138:1143	arg1	appearance					1145:1154	a normal appearance	1136:1154	a normal appearance in structure and composition (OA-1)	1136:1190	We found a reduction in energy dissipation density up to 80% in severely degenerated (OARSI grade 4, OA-4) vs normal (OA-1) cartilage, and more importantly, we found that even cartilage with a normal appearance in structure and composition (OA-1) dissipated 50% less energy than healthy (control) load-bearing cartilage (HL0).
30660720	8	1	theme	stress-	1784:1790	arg1	measures					1811:1818	stress- or stiffness-based measures	1784:1818	stress- or stiffness-based measures	1784:1818	We highlight the potential of energy dissipation, as opposed to stress- or stiffness-based measures, as a marker to diagnose early-stage OA.
30660720	6	2	theme	degeneration	1503:1514	arg1	levels					1493:1498	different levels	1483:1498	different levels	1483:1498	Changes in stresses and stiffnesses were in general less pronounced and did not allow us to distinguish between healthy load-bearing controls and very early-stage OA (OA-1), or to distinguish consistently among different levels of degeneration, i.e., OARSI grades.
30660720	6	3	theme	load-bearing	1392:1403	arg1	controls					1405:1412	healthy load-bearing controls	1384:1412	healthy load-bearing controls	1384:1412	Changes in stresses and stiffnesses were in general less pronounced and did not allow us to distinguish between healthy load-bearing controls and very early-stage OA (OA-1), or to distinguish consistently among different levels of degeneration, i.e., OARSI grades.
30660720	1	4	dep	composition	105:115	arg1	The					101:103	The	101:103	The	101:103	OBJECTIVE The composition and structure of articular cartilage evolves during the development and progression of osteoarthritis (OA) resulting in changing mechanical responses.
30660720	5	5	theme	less	1207:1210	arg1	energy					1212:1217	50% less energy	1203:1217	50% less energy	1203:1217	We found a reduction in energy dissipation density up to 80% in severely degenerated (OARSI grade 4, OA-4) vs normal (OA-1) cartilage, and more importantly, we found that even cartilage with a normal appearance in structure and composition (OA-1) dissipated 50% less energy than healthy (control) load-bearing cartilage (HL0).
30660720	6	6	from	general	1316:1322	arg1	pronounced					1329:1338	pronounced	1329:1338	pronounced	1329:1338	Changes in stresses and stiffnesses were in general less pronounced and did not allow us to distinguish between healthy load-bearing controls and very early-stage OA (OA-1), or to distinguish consistently among different levels of degeneration, i.e., OARSI grades.
30660720	8	7	theme	stiffness-based	1795:1809	arg1	measures					1811:1818	stress- or stiffness-based measures	1784:1818	stress- or stiffness-based measures	1784:1818	We highlight the potential of energy dissipation, as opposed to stress- or stiffness-based measures, as a marker to diagnose early-stage OA.
30660720	3	8	from	donors	546:551	arg1	specimens					527:535	64 specimens	524:535	64 specimens from ten donors undergoing total knee arthroplasty (TKA)	524:592	DESIGN We completed quasi-static, large-strain shear tests on 64 specimens from ten donors undergoing total knee arthroplasty (TKA), and quantified the corresponding state of OA (OARSI grade), structural integrity (PLM score), and composition (glycosaminoglycan and collagen content).
30660720	4	9	theme	nonlinear	767:775	arg1	relationships					791:803	nonlinear stress-strain relationships	767:803	nonlinear stress-strain relationships with distinct hystereses for all magnitudes of applied strain where stiffnesses, nonlinearities, and hystereses all reduced as OA advanced	767:942	RESULTS We observed nonlinear stress-strain relationships with distinct hystereses for all magnitudes of applied strain where stiffnesses, nonlinearities, and hystereses all reduced as OA advanced.
30660720	5	10	theme	energy	969:974	arg1	density					988:994	energy dissipation density	969:994	energy dissipation density	969:994	We found a reduction in energy dissipation density up to 80% in severely degenerated (OARSI grade 4, OA-4) vs normal (OA-1) cartilage, and more importantly, we found that even cartilage with a normal appearance in structure and composition (OA-1) dissipated 50% less energy than healthy (control) load-bearing cartilage (HL0).
30660720	2	11	theme	mechanics	332:340	arg1	evolution					291:299	the evolution	287:299	the evolution of the intrinsic, large-strain mechanics of human articular cartilage-governed by collagen and proteoglycan	287:407	We aimed to assess the evolution of the intrinsic, large-strain mechanics of human articular cartilage-governed by collagen and proteoglycan and their interactions-during the progression of OA.
30660720	2	11	theme	mechanics	332:340	arg1	progression					443:453	their interactions-during the progression	413:453	their interactions-during the progression of OA	413:459	We aimed to assess the evolution of the intrinsic, large-strain mechanics of human articular cartilage-governed by collagen and proteoglycan and their interactions-during the progression of OA.
30660720	3	12	theme	collagen	728:735	arg1	content					737:743	collagen content	728:743	collagen content	728:743	DESIGN We completed quasi-static, large-strain shear tests on 64 specimens from ten donors undergoing total knee arthroplasty (TKA), and quantified the corresponding state of OA (OARSI grade), structural integrity (PLM score), and composition (glycosaminoglycan and collagen content).
30660720	7	13	theme	degeneration	1706:1717	arg1	signs					1697:1701	visible signs	1689:1701	visible signs of degeneration	1689:1717	CONCLUSIONS Our results suggest that reductions in energy dissipation density can be detected by bulk-tissue testing, and that these reductions precede visible signs of degeneration.
30660720	8	14	theme	energy	1750:1755	arg1	dissipation					1757:1767	energy dissipation	1750:1767	energy dissipation	1750:1767	We highlight the potential of energy dissipation, as opposed to stress- or stiffness-based measures, as a marker to diagnose early-stage OA.
30660720	6	15	dep	grades	1529:1534	arg1	i.e.					1517:1520	i.e.	1517:1520	i.e.	1517:1520	Changes in stresses and stiffnesses were in general less pronounced and did not allow us to distinguish between healthy load-bearing controls and very early-stage OA (OA-1), or to distinguish consistently among different levels of degeneration, i.e., OARSI grades.
30660720	7	16	theme	bulk-tissue	1634:1644	arg1	testing					1646:1652	bulk-tissue testing	1634:1652	bulk-tissue testing	1634:1652	CONCLUSIONS Our results suggest that reductions in energy dissipation density can be detected by bulk-tissue testing, and that these reductions precede visible signs of degeneration.
30660720	5	17	from	reduction	956:964	arg1	density					988:994	energy dissipation density	969:994	energy dissipation density	969:994	We found a reduction in energy dissipation density up to 80% in severely degenerated (OARSI grade 4, OA-4) vs normal (OA-1) cartilage, and more importantly, we found that even cartilage with a normal appearance in structure and composition (OA-1) dissipated 50% less energy than healthy (control) load-bearing cartilage (HL0).
30660720	3	18	theme	knee	570:573	arg1	TKA					589:591	TKA	589:591	TKA	589:591	DESIGN We completed quasi-static, large-strain shear tests on 64 specimens from ten donors undergoing total knee arthroplasty (TKA), and quantified the corresponding state of OA (OARSI grade), structural integrity (PLM score), and composition (glycosaminoglycan and collagen content).
30660720	3	18	theme	knee	570:573	arg1	arthroplasty					575:586	total knee arthroplasty	564:586	total knee arthroplasty (TKA)	564:592	DESIGN We completed quasi-static, large-strain shear tests on 64 specimens from ten donors undergoing total knee arthroplasty (TKA), and quantified the corresponding state of OA (OARSI grade), structural integrity (PLM score), and composition (glycosaminoglycan and collagen content).
30660720	5	19	dep	degenerated	1018:1028	arg1	OA-4					1046:1049	OA-4	1046:1049	OA-4	1046:1049	We found a reduction in energy dissipation density up to 80% in severely degenerated (OARSI grade 4, OA-4) vs normal (OA-1) cartilage, and more importantly, we found that even cartilage with a normal appearance in structure and composition (OA-1) dissipated 50% less energy than healthy (control) load-bearing cartilage (HL0).
30660720	5	19	dep	degenerated	1018:1028	arg1	OA-1					1063:1066	OA-1	1063:1066	OA-1	1063:1066	We found a reduction in energy dissipation density up to 80% in severely degenerated (OARSI grade 4, OA-4) vs normal (OA-1) cartilage, and more importantly, we found that even cartilage with a normal appearance in structure and composition (OA-1) dissipated 50% less energy than healthy (control) load-bearing cartilage (HL0).
30660720	5	19	dep	degenerated	1018:1028	arg1	grade					1037:1041	OARSI grade 4	1031:1043	OARSI grade 4	1031:1043	We found a reduction in energy dissipation density up to 80% in severely degenerated (OARSI grade 4, OA-4) vs normal (OA-1) cartilage, and more importantly, we found that even cartilage with a normal appearance in structure and composition (OA-1) dissipated 50% less energy than healthy (control) load-bearing cartilage (HL0).
30660720	4	20	dep	RESULTS	747:753	arg1	observed					758:765	observed	758:765	observed nonlinear stress-strain relationships with distinct hystereses for all magnitudes of applied strain where stiffnesses, nonlinearities, and hystereses all reduced as OA advanced	758:942	RESULTS We observed nonlinear stress-strain relationships with distinct hystereses for all magnitudes of applied strain where stiffnesses, nonlinearities, and hystereses all reduced as OA advanced.
30660720	4	21	theme	strain	860:865	arg1	magnitudes					838:847	all magnitudes	834:847	all magnitudes of applied strain	834:865	RESULTS We observed nonlinear stress-strain relationships with distinct hystereses for all magnitudes of applied strain where stiffnesses, nonlinearities, and hystereses all reduced as OA advanced.
30660720	1	22	theme	OBJECTIVE	91:99	arg1	composition					105:115	composition	105:115	composition	105:115	OBJECTIVE The composition and structure of articular cartilage evolves during the development and progression of osteoarthritis (OA) resulting in changing mechanical responses.
30660720	2	23	theme	interactions-during	419:437	arg1	progression					443:453	their interactions-during the progression	413:453	their interactions-during the progression of OA	413:459	We aimed to assess the evolution of the intrinsic, large-strain mechanics of human articular cartilage-governed by collagen and proteoglycan and their interactions-during the progression of OA.
30660720	6	24	theme	early-stage	1423:1433	arg1	OA-1					1439:1442	OA-1	1439:1442	OA-1	1439:1442	Changes in stresses and stiffnesses were in general less pronounced and did not allow us to distinguish between healthy load-bearing controls and very early-stage OA (OA-1), or to distinguish consistently among different levels of degeneration, i.e., OARSI grades.
30660720	6	24	theme	early-stage	1423:1433	arg1	OA					1435:1436	very early-stage OA	1418:1436	very early-stage OA (OA-1)	1418:1443	Changes in stresses and stiffnesses were in general less pronounced and did not allow us to distinguish between healthy load-bearing controls and very early-stage OA (OA-1), or to distinguish consistently among different levels of degeneration, i.e., OARSI grades.
30660720	7	25	dep	CONCLUSIONS	1537:1547	arg1	suggest					1561:1567	suggest	1561:1567	suggest that reductions in energy dissipation density can be detected by bulk-tissue testing, and that these reductions precede visible signs of degeneration	1561:1717	CONCLUSIONS Our results suggest that reductions in energy dissipation density can be detected by bulk-tissue testing, and that these reductions precede visible signs of degeneration.
30660720	1	26	dep	development	173:183	arg1	the					169:171	the	169:171	the	169:171	OBJECTIVE The composition and structure of articular cartilage evolves during the development and progression of osteoarthritis (OA) resulting in changing mechanical responses.
30660720	5	27	from	%	1004:1004	arg1	cartilage					1069:1077	severely degenerated (OARSI grade 4, OA-4) vs normal (OA-1) cartilage	1009:1077	severely degenerated (OARSI grade 4, OA-4) vs normal (OA-1) cartilage	1009:1077	We found a reduction in energy dissipation density up to 80% in severely degenerated (OARSI grade 4, OA-4) vs normal (OA-1) cartilage, and more importantly, we found that even cartilage with a normal appearance in structure and composition (OA-1) dissipated 50% less energy than healthy (control) load-bearing cartilage (HL0).
30660720	1	28	theme	osteoarthritis	204:217	arg1	progression					189:199	progression	189:199	progression	189:199	OBJECTIVE The composition and structure of articular cartilage evolves during the development and progression of osteoarthritis (OA) resulting in changing mechanical responses.
30660720	1	28	theme	osteoarthritis	204:217	arg1	development					173:183	development	173:183	development	173:183	OBJECTIVE The composition and structure of articular cartilage evolves during the development and progression of osteoarthritis (OA) resulting in changing mechanical responses.
30660720	4	29	with	relationships	791:803	arg1	hystereses					819:828	distinct hystereses	810:828	distinct hystereses for all magnitudes of applied strain where stiffnesses, nonlinearities, and hystereses all reduced as OA advanced	810:942	RESULTS We observed nonlinear stress-strain relationships with distinct hystereses for all magnitudes of applied strain where stiffnesses, nonlinearities, and hystereses all reduced as OA advanced.
30660720	3	30	theme	PLM	677:679	arg1	integrity					666:674	structural integrity	655:674	structural integrity (PLM score)	655:686	DESIGN We completed quasi-static, large-strain shear tests on 64 specimens from ten donors undergoing total knee arthroplasty (TKA), and quantified the corresponding state of OA (OARSI grade), structural integrity (PLM score), and composition (glycosaminoglycan and collagen content).
30660720	3	30	theme	PLM	677:679	arg1	score					681:685	PLM score	677:685	PLM score	677:685	DESIGN We completed quasi-static, large-strain shear tests on 64 specimens from ten donors undergoing total knee arthroplasty (TKA), and quantified the corresponding state of OA (OARSI grade), structural integrity (PLM score), and composition (glycosaminoglycan and collagen content).
30660720	6	31	theme	healthy	1384:1390	arg1	controls					1405:1412	healthy load-bearing controls	1384:1412	healthy load-bearing controls	1384:1412	Changes in stresses and stiffnesses were in general less pronounced and did not allow us to distinguish between healthy load-bearing controls and very early-stage OA (OA-1), or to distinguish consistently among different levels of degeneration, i.e., OARSI grades.
30660720	7	32	theme	energy	1588:1593	arg1	density					1607:1613	energy dissipation density	1588:1613	energy dissipation density	1588:1613	CONCLUSIONS Our results suggest that reductions in energy dissipation density can be detected by bulk-tissue testing, and that these reductions precede visible signs of degeneration.
30660720	5	33	theme	normal	1055:1060	arg1	cartilage					1069:1077	severely degenerated (OARSI grade 4, OA-4) vs normal (OA-1) cartilage	1009:1077	severely degenerated (OARSI grade 4, OA-4) vs normal (OA-1) cartilage	1009:1077	We found a reduction in energy dissipation density up to 80% in severely degenerated (OARSI grade 4, OA-4) vs normal (OA-1) cartilage, and more importantly, we found that even cartilage with a normal appearance in structure and composition (OA-1) dissipated 50% less energy than healthy (control) load-bearing cartilage (HL0).
30660720	0	34	theme	shear	26:30	arg1	responses					32:40	large-strain shear responses	13:40	large-strain shear responses of progressively osteoarthritic human cartilage	13:88	The evolving large-strain shear responses of progressively osteoarthritic human cartilage.
30660720	7	35	theme	dissipation	1595:1605	arg1	density					1607:1613	energy dissipation density	1588:1613	energy dissipation density	1588:1613	CONCLUSIONS Our results suggest that reductions in energy dissipation density can be detected by bulk-tissue testing, and that these reductions precede visible signs of degeneration.
30660720	3	36	dep	quasi-static	482:493	arg1	large-strain					496:507	large-strain	496:507	large-strain	496:507	DESIGN We completed quasi-static, large-strain shear tests on 64 specimens from ten donors undergoing total knee arthroplasty (TKA), and quantified the corresponding state of OA (OARSI grade), structural integrity (PLM score), and composition (glycosaminoglycan and collagen content).
30660720	6	37	from	pronounced	1329:1338	arg1	general					1316:1322	general	1316:1322	general	1316:1322	Changes in stresses and stiffnesses were in general less pronounced and did not allow us to distinguish between healthy load-bearing controls and very early-stage OA (OA-1), or to distinguish consistently among different levels of degeneration, i.e., OARSI grades.
30660720	5	38	theme	degenerated	1018:1028	arg1	cartilage					1069:1077	severely degenerated (OARSI grade 4, OA-4) vs normal (OA-1) cartilage	1009:1077	severely degenerated (OARSI grade 4, OA-4) vs normal (OA-1) cartilage	1009:1077	We found a reduction in energy dissipation density up to 80% in severely degenerated (OARSI grade 4, OA-4) vs normal (OA-1) cartilage, and more importantly, we found that even cartilage with a normal appearance in structure and composition (OA-1) dissipated 50% less energy than healthy (control) load-bearing cartilage (HL0).
30660720	3	39	theme	shear	509:513	arg1	tests					515:519	quasi-static, large-strain shear tests	482:519	quasi-static, large-strain shear tests on 64 specimens from ten donors undergoing total knee arthroplasty (TKA)	482:592	DESIGN We completed quasi-static, large-strain shear tests on 64 specimens from ten donors undergoing total knee arthroplasty (TKA), and quantified the corresponding state of OA (OARSI grade), structural integrity (PLM score), and composition (glycosaminoglycan and collagen content).
30660720	0	40	theme	large-strain	13:24	arg1	responses					32:40	large-strain shear responses	13:40	large-strain shear responses of progressively osteoarthritic human cartilage	13:88	The evolving large-strain shear responses of progressively osteoarthritic human cartilage.
30660720	3	41	dep	composition	693:703	arg1	glycosaminoglycan					706:722	glycosaminoglycan	706:722	glycosaminoglycan	706:722	DESIGN We completed quasi-static, large-strain shear tests on 64 specimens from ten donors undergoing total knee arthroplasty (TKA), and quantified the corresponding state of OA (OARSI grade), structural integrity (PLM score), and composition (glycosaminoglycan and collagen content).
30660720	3	41	dep	composition	693:703	arg1	content					737:743	collagen content	728:743	collagen content	728:743	DESIGN We completed quasi-static, large-strain shear tests on 64 specimens from ten donors undergoing total knee arthroplasty (TKA), and quantified the corresponding state of OA (OARSI grade), structural integrity (PLM score), and composition (glycosaminoglycan and collagen content).
30660720	4	42	theme	advanced	935:942	arg1	OA					932:933	OA advanced	932:942	OA advanced	932:942	RESULTS We observed nonlinear stress-strain relationships with distinct hystereses for all magnitudes of applied strain where stiffnesses, nonlinearities, and hystereses all reduced as OA advanced.
30660720	3	43	theme	structural	655:664	arg1	integrity					666:674	structural integrity	655:674	structural integrity (PLM score)	655:686	DESIGN We completed quasi-static, large-strain shear tests on 64 specimens from ten donors undergoing total knee arthroplasty (TKA), and quantified the corresponding state of OA (OARSI grade), structural integrity (PLM score), and composition (glycosaminoglycan and collagen content).
30660720	3	43	theme	structural	655:664	arg1	score					681:685	PLM score	677:685	PLM score	677:685	DESIGN We completed quasi-static, large-strain shear tests on 64 specimens from ten donors undergoing total knee arthroplasty (TKA), and quantified the corresponding state of OA (OARSI grade), structural integrity (PLM score), and composition (glycosaminoglycan and collagen content).
30660720	2	44	theme	intrinsic	308:316	arg1	mechanics					332:340	the intrinsic, large-strain mechanics	304:340	the intrinsic, large-strain mechanics of human articular cartilage-governed by collagen and proteoglycan	304:407	We aimed to assess the evolution of the intrinsic, large-strain mechanics of human articular cartilage-governed by collagen and proteoglycan and their interactions-during the progression of OA.
30660720	5	45	theme	OARSI	1031:1035	arg1	OA-4					1046:1049	OA-4	1046:1049	OA-4	1046:1049	We found a reduction in energy dissipation density up to 80% in severely degenerated (OARSI grade 4, OA-4) vs normal (OA-1) cartilage, and more importantly, we found that even cartilage with a normal appearance in structure and composition (OA-1) dissipated 50% less energy than healthy (control) load-bearing cartilage (HL0).
30660720	5	45	theme	OARSI	1031:1035	arg1	grade					1037:1041	OARSI grade 4	1031:1043	OARSI grade 4	1031:1043	We found a reduction in energy dissipation density up to 80% in severely degenerated (OARSI grade 4, OA-4) vs normal (OA-1) cartilage, and more importantly, we found that even cartilage with a normal appearance in structure and composition (OA-1) dissipated 50% less energy than healthy (control) load-bearing cartilage (HL0).
30660720	2	46	theme	OA	458:459	arg1	evolution					291:299	the evolution	287:299	the evolution of the intrinsic, large-strain mechanics of human articular cartilage-governed by collagen and proteoglycan	287:407	We aimed to assess the evolution of the intrinsic, large-strain mechanics of human articular cartilage-governed by collagen and proteoglycan and their interactions-during the progression of OA.
30660720	2	46	theme	OA	458:459	arg1	progression					443:453	their interactions-during the progression	413:453	their interactions-during the progression of OA	413:459	We aimed to assess the evolution of the intrinsic, large-strain mechanics of human articular cartilage-governed by collagen and proteoglycan and their interactions-during the progression of OA.
30660720	2	47	dep	intrinsic	308:316	arg1	large-strain					319:330	large-strain	319:330	large-strain	319:330	We aimed to assess the evolution of the intrinsic, large-strain mechanics of human articular cartilage-governed by collagen and proteoglycan and their interactions-during the progression of OA.
30660720	8	48	theme	early-stage	1845:1855	arg1	OA					1857:1858	early-stage OA	1845:1858	early-stage OA	1845:1858	We highlight the potential of energy dissipation, as opposed to stress- or stiffness-based measures, as a marker to diagnose early-stage OA.
30660720	3	49	theme	integrity	666:674	arg1	state					628:632	the corresponding state	610:632	the corresponding state of OA (OARSI grade), structural integrity (PLM score), and composition (glycosaminoglycan and collagen content)	610:744	DESIGN We completed quasi-static, large-strain shear tests on 64 specimens from ten donors undergoing total knee arthroplasty (TKA), and quantified the corresponding state of OA (OARSI grade), structural integrity (PLM score), and composition (glycosaminoglycan and collagen content).
30660720	3	50	dep	DESIGN	462:467	arg1	completed					472:480	completed	472:480	completed quasi-static, large-strain shear tests on 64 specimens from ten donors undergoing total knee arthroplasty (TKA)	472:592	DESIGN We completed quasi-static, large-strain shear tests on 64 specimens from ten donors undergoing total knee arthroplasty (TKA), and quantified the corresponding state of OA (OARSI grade), structural integrity (PLM score), and composition (glycosaminoglycan and collagen content).
30660720	3	50	dep	DESIGN	462:467	arg1	quantified					599:608	quantified	599:608	quantified the corresponding state of OA (OARSI grade), structural integrity (PLM score), and composition (glycosaminoglycan and collagen content)	599:744	DESIGN We completed quasi-static, large-strain shear tests on 64 specimens from ten donors undergoing total knee arthroplasty (TKA), and quantified the corresponding state of OA (OARSI grade), structural integrity (PLM score), and composition (glycosaminoglycan and collagen content).
30660720	5	51	theme	control	1233:1239	arg1	cartilage					1255:1263	healthy (control) load-bearing cartilage	1224:1263	healthy (control) load-bearing cartilage (HL0)	1224:1269	We found a reduction in energy dissipation density up to 80% in severely degenerated (OARSI grade 4, OA-4) vs normal (OA-1) cartilage, and more importantly, we found that even cartilage with a normal appearance in structure and composition (OA-1) dissipated 50% less energy than healthy (control) load-bearing cartilage (HL0).
30660720	5	51	theme	control	1233:1239	arg1	HL0					1266:1268	HL0	1266:1268	HL0	1266:1268	We found a reduction in energy dissipation density up to 80% in severely degenerated (OARSI grade 4, OA-4) vs normal (OA-1) cartilage, and more importantly, we found that even cartilage with a normal appearance in structure and composition (OA-1) dissipated 50% less energy than healthy (control) load-bearing cartilage (HL0).
30660720	5	52	from	appearance	1145:1154	arg1	OA-1					1186:1189	OA-1	1186:1189	OA-1	1186:1189	We found a reduction in energy dissipation density up to 80% in severely degenerated (OARSI grade 4, OA-4) vs normal (OA-1) cartilage, and more importantly, we found that even cartilage with a normal appearance in structure and composition (OA-1) dissipated 50% less energy than healthy (control) load-bearing cartilage (HL0).
30660720	5	52	from	appearance	1145:1154	arg1	composition					1173:1183	composition	1173:1183	composition	1173:1183	We found a reduction in energy dissipation density up to 80% in severely degenerated (OARSI grade 4, OA-4) vs normal (OA-1) cartilage, and more importantly, we found that even cartilage with a normal appearance in structure and composition (OA-1) dissipated 50% less energy than healthy (control) load-bearing cartilage (HL0).
30660720	5	52	from	appearance	1145:1154	arg1	structure					1159:1167	structure	1159:1167	structure	1159:1167	We found a reduction in energy dissipation density up to 80% in severely degenerated (OARSI grade 4, OA-4) vs normal (OA-1) cartilage, and more importantly, we found that even cartilage with a normal appearance in structure and composition (OA-1) dissipated 50% less energy than healthy (control) load-bearing cartilage (HL0).
30660720	6	53	theme	different	1483:1491	arg1	levels					1493:1498	different levels	1483:1498	different levels	1483:1498	Changes in stresses and stiffnesses were in general less pronounced and did not allow us to distinguish between healthy load-bearing controls and very early-stage OA (OA-1), or to distinguish consistently among different levels of degeneration, i.e., OARSI grades.
30660720	1	54	theme	mechanical	246:255	arg1	responses					257:265	mechanical responses	246:265	mechanical responses	246:265	OBJECTIVE The composition and structure of articular cartilage evolves during the development and progression of osteoarthritis (OA) resulting in changing mechanical responses.
30660720	0	55	theme	osteoarthritic	59:72	arg1	cartilage					80:88	progressively osteoarthritic human cartilage	45:88	progressively osteoarthritic human cartilage	45:88	The evolving large-strain shear responses of progressively osteoarthritic human cartilage.
30660720	4	56	theme	applied	852:858	arg1	strain					860:865	applied strain	852:865	applied strain	852:865	RESULTS We observed nonlinear stress-strain relationships with distinct hystereses for all magnitudes of applied strain where stiffnesses, nonlinearities, and hystereses all reduced as OA advanced.
30660720	2	57	theme	cartilage-governed	361:378	arg1	mechanics					332:340	the intrinsic, large-strain mechanics	304:340	the intrinsic, large-strain mechanics of human articular cartilage-governed by collagen and proteoglycan	304:407	We aimed to assess the evolution of the intrinsic, large-strain mechanics of human articular cartilage-governed by collagen and proteoglycan and their interactions-during the progression of OA.
30660720	5	58	theme	even	1116:1119	arg1	cartilage					1121:1129	even cartilage	1116:1129	even cartilage with a normal appearance in structure and composition (OA-1)	1116:1190	We found a reduction in energy dissipation density up to 80% in severely degenerated (OARSI grade 4, OA-4) vs normal (OA-1) cartilage, and more importantly, we found that even cartilage with a normal appearance in structure and composition (OA-1) dissipated 50% less energy than healthy (control) load-bearing cartilage (HL0).
30660720	6	59	from	Changes	1272:1278	arg1	stiffnesses					1296:1306	stiffnesses	1296:1306	stiffnesses	1296:1306	Changes in stresses and stiffnesses were in general less pronounced and did not allow us to distinguish between healthy load-bearing controls and very early-stage OA (OA-1), or to distinguish consistently among different levels of degeneration, i.e., OARSI grades.
30660720	6	59	from	Changes	1272:1278	arg1	stresses					1283:1290	stresses	1283:1290	stresses	1283:1290	Changes in stresses and stiffnesses were in general less pronounced and did not allow us to distinguish between healthy load-bearing controls and very early-stage OA (OA-1), or to distinguish consistently among different levels of degeneration, i.e., OARSI grades.
30660720	3	60	theme	corresponding	614:626	arg1	state					628:632	the corresponding state	610:632	the corresponding state of OA (OARSI grade), structural integrity (PLM score), and composition (glycosaminoglycan and collagen content)	610:744	DESIGN We completed quasi-static, large-strain shear tests on 64 specimens from ten donors undergoing total knee arthroplasty (TKA), and quantified the corresponding state of OA (OARSI grade), structural integrity (PLM score), and composition (glycosaminoglycan and collagen content).
30660720	1	61	theme	articular	134:142	arg1	cartilage					144:152	articular cartilage	134:152	articular cartilage	134:152	OBJECTIVE The composition and structure of articular cartilage evolves during the development and progression of osteoarthritis (OA) resulting in changing mechanical responses.
30660720	7	62	from	reductions	1574:1583	arg1	density					1607:1613	energy dissipation density	1588:1613	energy dissipation density	1588:1613	CONCLUSIONS Our results suggest that reductions in energy dissipation density can be detected by bulk-tissue testing, and that these reductions precede visible signs of degeneration.
30660720	6	63	theme	OARSI	1523:1527	arg1	grades					1529:1534	OARSI grades	1523:1534	OARSI grades	1523:1534	Changes in stresses and stiffnesses were in general less pronounced and did not allow us to distinguish between healthy load-bearing controls and very early-stage OA (OA-1), or to distinguish consistently among different levels of degeneration, i.e., OARSI grades.
30660720	4	64	theme	distinct	810:817	arg1	hystereses					819:828	distinct hystereses	810:828	distinct hystereses for all magnitudes of applied strain where stiffnesses, nonlinearities, and hystereses all reduced as OA advanced	810:942	RESULTS We observed nonlinear stress-strain relationships with distinct hystereses for all magnitudes of applied strain where stiffnesses, nonlinearities, and hystereses all reduced as OA advanced.
30660720	3	65	theme	OARSI	641:645	arg1	OA					637:638	OA	637:638	OA (OARSI grade)	637:652	DESIGN We completed quasi-static, large-strain shear tests on 64 specimens from ten donors undergoing total knee arthroplasty (TKA), and quantified the corresponding state of OA (OARSI grade), structural integrity (PLM score), and composition (glycosaminoglycan and collagen content).
30660720	3	65	theme	OARSI	641:645	arg1	grade					647:651	OARSI grade	641:651	OARSI grade	641:651	DESIGN We completed quasi-static, large-strain shear tests on 64 specimens from ten donors undergoing total knee arthroplasty (TKA), and quantified the corresponding state of OA (OARSI grade), structural integrity (PLM score), and composition (glycosaminoglycan and collagen content).
30660720	3	66	from	tests	515:519	arg1	specimens					527:535	64 specimens	524:535	64 specimens from ten donors undergoing total knee arthroplasty (TKA)	524:592	DESIGN We completed quasi-static, large-strain shear tests on 64 specimens from ten donors undergoing total knee arthroplasty (TKA), and quantified the corresponding state of OA (OARSI grade), structural integrity (PLM score), and composition (glycosaminoglycan and collagen content).
30660720	1	67	theme	cartilage	144:152	arg1	composition					105:115	composition	105:115	composition	105:115	OBJECTIVE The composition and structure of articular cartilage evolves during the development and progression of osteoarthritis (OA) resulting in changing mechanical responses.
30660720	1	67	theme	cartilage	144:152	arg1	structure					121:129	structure	121:129	structure	121:129	OBJECTIVE The composition and structure of articular cartilage evolves during the development and progression of osteoarthritis (OA) resulting in changing mechanical responses.
30660720	5	68	theme	healthy	1224:1230	arg1	cartilage					1255:1263	healthy (control) load-bearing cartilage	1224:1263	healthy (control) load-bearing cartilage (HL0)	1224:1269	We found a reduction in energy dissipation density up to 80% in severely degenerated (OARSI grade 4, OA-4) vs normal (OA-1) cartilage, and more importantly, we found that even cartilage with a normal appearance in structure and composition (OA-1) dissipated 50% less energy than healthy (control) load-bearing cartilage (HL0).
30660720	5	68	theme	healthy	1224:1230	arg1	HL0					1266:1268	HL0	1266:1268	HL0	1266:1268	We found a reduction in energy dissipation density up to 80% in severely degenerated (OARSI grade 4, OA-4) vs normal (OA-1) cartilage, and more importantly, we found that even cartilage with a normal appearance in structure and composition (OA-1) dissipated 50% less energy than healthy (control) load-bearing cartilage (HL0).
30660720	5	69	dep	%	1004:1004	arg1	up					996:997	up	996:997	up	996:997	We found a reduction in energy dissipation density up to 80% in severely degenerated (OARSI grade 4, OA-4) vs normal (OA-1) cartilage, and more importantly, we found that even cartilage with a normal appearance in structure and composition (OA-1) dissipated 50% less energy than healthy (control) load-bearing cartilage (HL0).
30660720	4	70	theme	stress-strain	777:789	arg1	relationships					791:803	nonlinear stress-strain relationships	767:803	nonlinear stress-strain relationships with distinct hystereses for all magnitudes of applied strain where stiffnesses, nonlinearities, and hystereses all reduced as OA advanced	767:942	RESULTS We observed nonlinear stress-strain relationships with distinct hystereses for all magnitudes of applied strain where stiffnesses, nonlinearities, and hystereses all reduced as OA advanced.
30660720	7	71	theme	visible	1689:1695	arg1	signs					1697:1701	visible signs	1689:1701	visible signs of degeneration	1689:1717	CONCLUSIONS Our results suggest that reductions in energy dissipation density can be detected by bulk-tissue testing, and that these reductions precede visible signs of degeneration.
30660720	5	72	theme	dissipation	976:986	arg1	density					988:994	energy dissipation density	969:994	energy dissipation density	969:994	We found a reduction in energy dissipation density up to 80% in severely degenerated (OARSI grade 4, OA-4) vs normal (OA-1) cartilage, and more importantly, we found that even cartilage with a normal appearance in structure and composition (OA-1) dissipated 50% less energy than healthy (control) load-bearing cartilage (HL0).
30660720	5	73	theme	load-bearing	1242:1253	arg1	cartilage					1255:1263	healthy (control) load-bearing cartilage	1224:1263	healthy (control) load-bearing cartilage (HL0)	1224:1269	We found a reduction in energy dissipation density up to 80% in severely degenerated (OARSI grade 4, OA-4) vs normal (OA-1) cartilage, and more importantly, we found that even cartilage with a normal appearance in structure and composition (OA-1) dissipated 50% less energy than healthy (control) load-bearing cartilage (HL0).
30660720	5	73	theme	load-bearing	1242:1253	arg1	HL0					1266:1268	HL0	1266:1268	HL0	1266:1268	We found a reduction in energy dissipation density up to 80% in severely degenerated (OARSI grade 4, OA-4) vs normal (OA-1) cartilage, and more importantly, we found that even cartilage with a normal appearance in structure and composition (OA-1) dissipated 50% less energy than healthy (control) load-bearing cartilage (HL0).
30660720	2	74	theme	human	345:349	arg1	cartilage-governed					361:378	human articular cartilage-governed	345:378	human articular cartilage-governed by collagen and proteoglycan	345:407	We aimed to assess the evolution of the intrinsic, large-strain mechanics of human articular cartilage-governed by collagen and proteoglycan and their interactions-during the progression of OA.
30660720	0	75	theme	cartilage	80:88	arg1	responses					32:40	large-strain shear responses	13:40	large-strain shear responses of progressively osteoarthritic human cartilage	13:88	The evolving large-strain shear responses of progressively osteoarthritic human cartilage.
30660720	2	76	theme	articular	351:359	arg1	cartilage-governed					361:378	human articular cartilage-governed	345:378	human articular cartilage-governed by collagen and proteoglycan	345:407	We aimed to assess the evolution of the intrinsic, large-strain mechanics of human articular cartilage-governed by collagen and proteoglycan and their interactions-during the progression of OA.
30660720	3	77	theme	OA	637:638	arg1	state					628:632	the corresponding state	610:632	the corresponding state of OA (OARSI grade), structural integrity (PLM score), and composition (glycosaminoglycan and collagen content)	610:744	DESIGN We completed quasi-static, large-strain shear tests on 64 specimens from ten donors undergoing total knee arthroplasty (TKA), and quantified the corresponding state of OA (OARSI grade), structural integrity (PLM score), and composition (glycosaminoglycan and collagen content).
30660720	3	78	theme	quasi-static	482:493	arg1	tests					515:519	quasi-static, large-strain shear tests	482:519	quasi-static, large-strain shear tests on 64 specimens from ten donors undergoing total knee arthroplasty (TKA)	482:592	DESIGN We completed quasi-static, large-strain shear tests on 64 specimens from ten donors undergoing total knee arthroplasty (TKA), and quantified the corresponding state of OA (OARSI grade), structural integrity (PLM score), and composition (glycosaminoglycan and collagen content).
30660720	0	79	theme	human	74:78	arg1	cartilage					80:88	progressively osteoarthritic human cartilage	45:88	progressively osteoarthritic human cartilage	45:88	The evolving large-strain shear responses of progressively osteoarthritic human cartilage.
30660720	3	80	theme	composition	693:703	arg1	state					628:632	the corresponding state	610:632	the corresponding state of OA (OARSI grade), structural integrity (PLM score), and composition (glycosaminoglycan and collagen content)	610:744	DESIGN We completed quasi-static, large-strain shear tests on 64 specimens from ten donors undergoing total knee arthroplasty (TKA), and quantified the corresponding state of OA (OARSI grade), structural integrity (PLM score), and composition (glycosaminoglycan and collagen content).
30660720	5	81	with	cartilage	1121:1129	arg1	appearance					1145:1154	a normal appearance	1136:1154	a normal appearance in structure and composition (OA-1)	1136:1190	We found a reduction in energy dissipation density up to 80% in severely degenerated (OARSI grade 4, OA-4) vs normal (OA-1) cartilage, and more importantly, we found that even cartilage with a normal appearance in structure and composition (OA-1) dissipated 50% less energy than healthy (control) load-bearing cartilage (HL0).
30660720	3	82	theme	total	564:568	arg1	TKA					589:591	TKA	589:591	TKA	589:591	DESIGN We completed quasi-static, large-strain shear tests on 64 specimens from ten donors undergoing total knee arthroplasty (TKA), and quantified the corresponding state of OA (OARSI grade), structural integrity (PLM score), and composition (glycosaminoglycan and collagen content).
30660720	3	82	theme	total	564:568	arg1	arthroplasty					575:586	total knee arthroplasty	564:586	total knee arthroplasty (TKA)	564:592	DESIGN We completed quasi-static, large-strain shear tests on 64 specimens from ten donors undergoing total knee arthroplasty (TKA), and quantified the corresponding state of OA (OARSI grade), structural integrity (PLM score), and composition (glycosaminoglycan and collagen content).
30660720	8	83	theme	dissipation	1757:1767	arg1	marker					1826:1831	a marker	1824:1831	a marker to diagnose early-stage OA	1824:1858	We highlight the potential of energy dissipation, as opposed to stress- or stiffness-based measures, as a marker to diagnose early-stage OA.
30660720	8	83	theme	dissipation	1757:1767	arg1	potential					1737:1745	the potential	1733:1745	the potential of energy dissipation	1733:1767	We highlight the potential of energy dissipation, as opposed to stress- or stiffness-based measures, as a marker to diagnose early-stage OA.
29808843	6	0	theme	clotting	1117:1124	arg1	time					1126:1129	the prolonged thrombin clotting time	1094:1129	the prolonged thrombin clotting time	1094:1129	In addition, the prolonged thrombin clotting time, prothrombin time (PT), and activated partial thromboplastin time (aPTT) of TBA15/TBA29-GO were at least 2 times longer than those of commercially available drugs (heparin, argatroban, hirudin, and warfarin).
29808843	6	0	theme	clotting	1117:1124	arg1	aPTT					1198:1201	aPTT	1198:1201	aPTT	1198:1201	In addition, the prolonged thrombin clotting time, prothrombin time (PT), and activated partial thromboplastin time (aPTT) of TBA15/TBA29-GO were at least 2 times longer than those of commercially available drugs (heparin, argatroban, hirudin, and warfarin).
29808843	6	0	theme	clotting	1117:1124	arg1	times					1238:1242	times	1238:1242	times	1238:1242	In addition, the prolonged thrombin clotting time, prothrombin time (PT), and activated partial thromboplastin time (aPTT) of TBA15/TBA29-GO were at least 2 times longer than those of commercially available drugs (heparin, argatroban, hirudin, and warfarin).
29808843	9	1	theme	disorders	1880:1888	arg1	treatment					1856:1864	the treatment	1852:1864	the treatment of thrombotic disorders	1852:1888	Our multivalent, oligonucleotide-modified GO nanocomposites are easy to prepare, cost-effective, and highly biocompatible and they show great potential as effective anticoagulants for the treatment of thrombotic disorders.
29808843	1	2	contain	has	103:105	arg1	oxide					92:96	Graphene oxide	83:96	Graphene oxide (GO)	83:101	Graphene oxide (GO) has unique structural properties, can effectively adsorb single-strand DNA through π-π stacking, hydrogen bonding and hydrophobic interactions, and is useful in many biotechnology applications.
29808843	1	2	contain	has	103:105	arg2	properties					125:134	unique structural properties	107:134	unique structural properties	107:134	Graphene oxide (GO) has unique structural properties, can effectively adsorb single-strand DNA through π-π stacking, hydrogen bonding and hydrophobic interactions, and is useful in many biotechnology applications.
29808843	6	3	theme	available	1278:1286	arg1	hirudin					1316:1322	hirudin	1316:1322	hirudin	1316:1322	In addition, the prolonged thrombin clotting time, prothrombin time (PT), and activated partial thromboplastin time (aPTT) of TBA15/TBA29-GO were at least 2 times longer than those of commercially available drugs (heparin, argatroban, hirudin, and warfarin).
29808843	6	3	theme	available	1278:1286	arg1	warfarin					1329:1336	warfarin	1329:1336	warfarin	1329:1336	In addition, the prolonged thrombin clotting time, prothrombin time (PT), and activated partial thromboplastin time (aPTT) of TBA15/TBA29-GO were at least 2 times longer than those of commercially available drugs (heparin, argatroban, hirudin, and warfarin).
29808843	6	3	theme	available	1278:1286	arg1	argatroban					1304:1313	argatroban	1304:1313	argatroban	1304:1313	In addition, the prolonged thrombin clotting time, prothrombin time (PT), and activated partial thromboplastin time (aPTT) of TBA15/TBA29-GO were at least 2 times longer than those of commercially available drugs (heparin, argatroban, hirudin, and warfarin).
29808843	6	3	theme	available	1278:1286	arg1	heparin					1295:1301	heparin	1295:1301	heparin	1295:1301	In addition, the prolonged thrombin clotting time, prothrombin time (PT), and activated partial thromboplastin time (aPTT) of TBA15/TBA29-GO were at least 2 times longer than those of commercially available drugs (heparin, argatroban, hirudin, and warfarin).
29808843	6	3	theme	available	1278:1286	arg1	drugs					1288:1292	commercially available drugs	1265:1292	commercially available drugs (heparin, argatroban, hirudin, and warfarin)	1265:1337	In addition, the prolonged thrombin clotting time, prothrombin time (PT), and activated partial thromboplastin time (aPTT) of TBA15/TBA29-GO were at least 2 times longer than those of commercially available drugs (heparin, argatroban, hirudin, and warfarin).
29808843	6	4	from	times	1238:1242	arg1	addition					1084:1091	addition	1084:1091	addition	1084:1091	In addition, the prolonged thrombin clotting time, prothrombin time (PT), and activated partial thromboplastin time (aPTT) of TBA15/TBA29-GO were at least 2 times longer than those of commercially available drugs (heparin, argatroban, hirudin, and warfarin).
29808843	8	5	used	used	1607:1610	arg2	heparin					1580:1586	heparin	1580:1586	heparin	1580:1586	The rat-tail bleeding assay of the hemostasis time and ex vivo PT and aPTT further revealed that TBA15/TBA29-GO is superior (>2-fold) to heparin, which is commonly used in the treatment and prevention of thrombotic diseases.
29808843	6	6	dep	drugs	1288:1292	arg1	hirudin					1316:1322	hirudin	1316:1322	hirudin	1316:1322	In addition, the prolonged thrombin clotting time, prothrombin time (PT), and activated partial thromboplastin time (aPTT) of TBA15/TBA29-GO were at least 2 times longer than those of commercially available drugs (heparin, argatroban, hirudin, and warfarin).
29808843	6	6	dep	drugs	1288:1292	arg1	warfarin					1329:1336	warfarin	1329:1336	warfarin	1329:1336	In addition, the prolonged thrombin clotting time, prothrombin time (PT), and activated partial thromboplastin time (aPTT) of TBA15/TBA29-GO were at least 2 times longer than those of commercially available drugs (heparin, argatroban, hirudin, and warfarin).
29808843	6	6	dep	drugs	1288:1292	arg1	argatroban					1304:1313	argatroban	1304:1313	argatroban	1304:1313	In addition, the prolonged thrombin clotting time, prothrombin time (PT), and activated partial thromboplastin time (aPTT) of TBA15/TBA29-GO were at least 2 times longer than those of commercially available drugs (heparin, argatroban, hirudin, and warfarin).
29808843	6	6	dep	drugs	1288:1292	arg1	heparin					1295:1301	heparin	1295:1301	heparin	1295:1301	In addition, the prolonged thrombin clotting time, prothrombin time (PT), and activated partial thromboplastin time (aPTT) of TBA15/TBA29-GO were at least 2 times longer than those of commercially available drugs (heparin, argatroban, hirudin, and warfarin).
29808843	6	6	dep	drugs	1288:1292	arg1	drugs					1288:1292	commercially available drugs	1265:1292	commercially available drugs (heparin, argatroban, hirudin, and warfarin)	1265:1337	In addition, the prolonged thrombin clotting time, prothrombin time (PT), and activated partial thromboplastin time (aPTT) of TBA15/TBA29-GO were at least 2 times longer than those of commercially available drugs (heparin, argatroban, hirudin, and warfarin).
29808843	7	7	theme	high	1402:1405	arg1	biocompatibility					1407:1422	the high biocompatibility	1398:1422	the high biocompatibility of TBA15/TBA29-GO	1398:1440	The in vitro cytotoxicity and hemolysis analyses revealed the high biocompatibility of TBA15/TBA29-GO.
29808843	1	8	from	applications	283:294	arg1	useful					254:259	useful	254:259	useful	254:259	Graphene oxide (GO) has unique structural properties, can effectively adsorb single-strand DNA through π-π stacking, hydrogen bonding and hydrophobic interactions, and is useful in many biotechnology applications.
29808843	3	9	theme	TBA15/TBA29-GO	526:539	arg1	composite					541:549	The TBA15/TBA29-GO composite	522:549	The TBA15/TBA29-GO composite	522:549	The TBA15/TBA29-GO composite was simply obtained by the self-assembly of TBA15/TBA29 hybrids on GO.
29808843	1	10	theme	single-strand	160:172	arg1	DNA					174:176	single-strand DNA	160:176	single-strand DNA	160:176	Graphene oxide (GO) has unique structural properties, can effectively adsorb single-strand DNA through π-π stacking, hydrogen bonding and hydrophobic interactions, and is useful in many biotechnology applications.
29808843	6	11	theme	activated	1159:1167	arg1	time					1192:1195	activated partial thromboplastin time	1159:1195	activated partial thromboplastin time	1159:1195	In addition, the prolonged thrombin clotting time, prothrombin time (PT), and activated partial thromboplastin time (aPTT) of TBA15/TBA29-GO were at least 2 times longer than those of commercially available drugs (heparin, argatroban, hirudin, and warfarin).
29808843	4	12	dep	=	802:802	arg1	M					816:816	2.9 × 10-12 M	804:816	2.9 × 10-12 M	804:816	The high density and appropriate orientation of TBA15/TBA29 on the GO surface enabled TBA15/TBA29-GO to acquire an ultrastrong binding affinity for thrombin (dissociation constant = 2.9 × 10-12 M).
29808843	7	13	theme	hemolysis	1370:1378	arg1	analyses					1380:1387	The in vitro cytotoxicity and hemolysis analyses	1340:1387	The in vitro cytotoxicity and hemolysis analyses	1340:1387	The in vitro cytotoxicity and hemolysis analyses revealed the high biocompatibility of TBA15/TBA29-GO.
29808843	1	14	from	useful	254:259	arg1	applications					283:294	many biotechnology applications	264:294	many biotechnology applications	264:294	Graphene oxide (GO) has unique structural properties, can effectively adsorb single-strand DNA through π-π stacking, hydrogen bonding and hydrophobic interactions, and is useful in many biotechnology applications.
29808843	4	15	from	density	631:637	arg1	surface					692:698	the GO surface	685:698	the GO surface	685:698	The high density and appropriate orientation of TBA15/TBA29 on the GO surface enabled TBA15/TBA29-GO to acquire an ultrastrong binding affinity for thrombin (dissociation constant = 2.9 × 10-12 M).
29808843	5	16	theme	bivalent	832:839	arg1	hybrids					865:871	bivalent TBA15h20A20/TBA29h20A20 hybrids	832:871	bivalent TBA15h20A20/TBA29h20A20 hybrids	832:871	Compared to bivalent TBA15h20A20/TBA29h20A20 hybrids, the TBA15/TBA29-GO composite exhibited a superior anticoagulant potency (ca. 10-fold) against thrombin-mediated coagulation as a result of steric blocking effects and a higher binding affinity for thrombin.
29808843	6	17	theme	2	1236:1236	arg1	time					1126:1129	the prolonged thrombin clotting time	1094:1129	the prolonged thrombin clotting time	1094:1129	In addition, the prolonged thrombin clotting time, prothrombin time (PT), and activated partial thromboplastin time (aPTT) of TBA15/TBA29-GO were at least 2 times longer than those of commercially available drugs (heparin, argatroban, hirudin, and warfarin).
29808843	6	17	theme	2	1236:1236	arg1	times					1238:1242	times	1238:1242	times	1238:1242	In addition, the prolonged thrombin clotting time, prothrombin time (PT), and activated partial thromboplastin time (aPTT) of TBA15/TBA29-GO were at least 2 times longer than those of commercially available drugs (heparin, argatroban, hirudin, and warfarin).
29808843	4	18	theme	appropriate	643:653	arg1	orientation					655:665	appropriate orientation	643:665	appropriate orientation	643:665	The high density and appropriate orientation of TBA15/TBA29 on the GO surface enabled TBA15/TBA29-GO to acquire an ultrastrong binding affinity for thrombin (dissociation constant = 2.9 × 10-12 M).
29808843	8	19	dep	ex	1498:1499	arg1	vivo					1501:1504	vivo	1501:1504	vivo	1501:1504	The rat-tail bleeding assay of the hemostasis time and ex vivo PT and aPTT further revealed that TBA15/TBA29-GO is superior (>2-fold) to heparin, which is commonly used in the treatment and prevention of thrombotic diseases.
29808843	3	20	theme	hybrids	607:613	arg1	self-assembly					578:590	the self-assembly	574:590	the self-assembly of TBA15/TBA29 hybrids on GO	574:619	The TBA15/TBA29-GO composite was simply obtained by the self-assembly of TBA15/TBA29 hybrids on GO.
29808843	1	21	theme	hydrogen	200:207	arg1	bonding					209:215	hydrogen bonding	200:215	hydrogen bonding	200:215	Graphene oxide (GO) has unique structural properties, can effectively adsorb single-strand DNA through π-π stacking, hydrogen bonding and hydrophobic interactions, and is useful in many biotechnology applications.
29808843	8	22	theme	rat-tail	1447:1454	arg1	assay					1465:1469	The rat-tail bleeding assay	1443:1469	The rat-tail bleeding assay of the hemostasis time and ex vivo PT and aPTT	1443:1516	The rat-tail bleeding assay of the hemostasis time and ex vivo PT and aPTT further revealed that TBA15/TBA29-GO is superior (>2-fold) to heparin, which is commonly used in the treatment and prevention of thrombotic diseases.
29808843	7	23	theme	in	1344:1345	arg1	cytotoxicity					1353:1364	in vitro cytotoxicity	1344:1364	in vitro cytotoxicity	1344:1364	The in vitro cytotoxicity and hemolysis analyses revealed the high biocompatibility of TBA15/TBA29-GO.
29808843	6	24	theme	TBA15/TBA29-GO	1207:1220	arg1	time					1144:1147	prothrombin time	1132:1147	prothrombin time (PT)	1132:1152	In addition, the prolonged thrombin clotting time, prothrombin time (PT), and activated partial thromboplastin time (aPTT) of TBA15/TBA29-GO were at least 2 times longer than those of commercially available drugs (heparin, argatroban, hirudin, and warfarin).
29808843	6	24	theme	TBA15/TBA29-GO	1207:1220	arg1	times					1238:1242	times	1238:1242	times	1238:1242	In addition, the prolonged thrombin clotting time, prothrombin time (PT), and activated partial thromboplastin time (aPTT) of TBA15/TBA29-GO were at least 2 times longer than those of commercially available drugs (heparin, argatroban, hirudin, and warfarin).
29808843	6	24	theme	TBA15/TBA29-GO	1207:1220	arg1	PT					1150:1151	PT	1150:1151	PT	1150:1151	In addition, the prolonged thrombin clotting time, prothrombin time (PT), and activated partial thromboplastin time (aPTT) of TBA15/TBA29-GO were at least 2 times longer than those of commercially available drugs (heparin, argatroban, hirudin, and warfarin).
29808843	6	24	theme	TBA15/TBA29-GO	1207:1220	arg1	time					1192:1195	activated partial thromboplastin time	1159:1195	activated partial thromboplastin time	1159:1195	In addition, the prolonged thrombin clotting time, prothrombin time (PT), and activated partial thromboplastin time (aPTT) of TBA15/TBA29-GO were at least 2 times longer than those of commercially available drugs (heparin, argatroban, hirudin, and warfarin).
29808843	6	24	theme	TBA15/TBA29-GO	1207:1220	arg1	time					1126:1129	the prolonged thrombin clotting time	1094:1129	the prolonged thrombin clotting time	1094:1129	In addition, the prolonged thrombin clotting time, prothrombin time (PT), and activated partial thromboplastin time (aPTT) of TBA15/TBA29-GO were at least 2 times longer than those of commercially available drugs (heparin, argatroban, hirudin, and warfarin).
29808843	6	24	theme	TBA15/TBA29-GO	1207:1220	arg1	aPTT					1198:1201	aPTT	1198:1201	aPTT	1198:1201	In addition, the prolonged thrombin clotting time, prothrombin time (PT), and activated partial thromboplastin time (aPTT) of TBA15/TBA29-GO were at least 2 times longer than those of commercially available drugs (heparin, argatroban, hirudin, and warfarin).
29808843	1	25	theme	structural	114:123	arg1	properties					125:134	unique structural properties	107:134	unique structural properties	107:134	Graphene oxide (GO) has unique structural properties, can effectively adsorb single-strand DNA through π-π stacking, hydrogen bonding and hydrophobic interactions, and is useful in many biotechnology applications.
29808843	2	26	from	fibrinogen	510:519	arg1	formation					485:493	the formation	481:493	the formation of fibrin from fibrinogen	481:519	In this study, we developed a thrombin-binding-aptamers (15- and 29-mer) conjugated graphene oxide (TBA15/TBA29-GO) composite for the efficient inhibition of thrombin activity towards the formation of fibrin from fibrinogen.
29808843	4	27	theme	GO	689:690	arg1	surface					692:698	the GO surface	685:698	the GO surface	685:698	The high density and appropriate orientation of TBA15/TBA29 on the GO surface enabled TBA15/TBA29-GO to acquire an ultrastrong binding affinity for thrombin (dissociation constant = 2.9 × 10-12 M).
29808843	9	28	dep	oligonucleotide-modified	1685:1708	arg1	GO					1710:1711	GO	1710:1711	GO	1710:1711	Our multivalent, oligonucleotide-modified GO nanocomposites are easy to prepare, cost-effective, and highly biocompatible and they show great potential as effective anticoagulants for the treatment of thrombotic disorders.
29808843	9	29	dep	multivalent	1672:1682	arg1	oligonucleotide-modified					1685:1708	oligonucleotide-modified	1685:1708	oligonucleotide-modified	1685:1708	Our multivalent, oligonucleotide-modified GO nanocomposites are easy to prepare, cost-effective, and highly biocompatible and they show great potential as effective anticoagulants for the treatment of thrombotic disorders.
29808843	5	30	theme	anticoagulant	924:936	arg1	result					1003:1008	a result	1001:1008	a result of steric blocking effects and a higher binding affinity for thrombin	1001:1078	Compared to bivalent TBA15h20A20/TBA29h20A20 hybrids, the TBA15/TBA29-GO composite exhibited a superior anticoagulant potency (ca. 10-fold) against thrombin-mediated coagulation as a result of steric blocking effects and a higher binding affinity for thrombin.
29808843	5	30	theme	anticoagulant	924:936	arg1	potency					938:944	a superior anticoagulant potency	913:944	a superior anticoagulant potency (ca. 10-fold) against thrombin-mediated coagulation	913:996	Compared to bivalent TBA15h20A20/TBA29h20A20 hybrids, the TBA15/TBA29-GO composite exhibited a superior anticoagulant potency (ca. 10-fold) against thrombin-mediated coagulation as a result of steric blocking effects and a higher binding affinity for thrombin.
29808843	8	31	theme	ex	1498:1499	arg1	PT					1506:1507	PT	1506:1507	PT	1506:1507	The rat-tail bleeding assay of the hemostasis time and ex vivo PT and aPTT further revealed that TBA15/TBA29-GO is superior (>2-fold) to heparin, which is commonly used in the treatment and prevention of thrombotic diseases.
29808843	9	32	dep	easy	1732:1735	arg1	biocompatible					1776:1788	biocompatible	1776:1788	biocompatible	1776:1788	Our multivalent, oligonucleotide-modified GO nanocomposites are easy to prepare, cost-effective, and highly biocompatible and they show great potential as effective anticoagulants for the treatment of thrombotic disorders.
29808843	8	33	theme	PT	1506:1507	arg1	assay					1465:1469	The rat-tail bleeding assay	1443:1469	The rat-tail bleeding assay of the hemostasis time and ex vivo PT and aPTT	1443:1516	The rat-tail bleeding assay of the hemostasis time and ex vivo PT and aPTT further revealed that TBA15/TBA29-GO is superior (>2-fold) to heparin, which is commonly used in the treatment and prevention of thrombotic diseases.
29808843	2	34	theme	graphene	381:388	arg1	TBA15/TBA29-GO					397:410	TBA15/TBA29-GO	397:410	TBA15/TBA29-GO	397:410	In this study, we developed a thrombin-binding-aptamers (15- and 29-mer) conjugated graphene oxide (TBA15/TBA29-GO) composite for the efficient inhibition of thrombin activity towards the formation of fibrin from fibrinogen.
29808843	2	34	theme	graphene	381:388	arg1	oxide					390:394	graphene oxide	381:394	graphene oxide (TBA15/TBA29-GO)	381:411	In this study, we developed a thrombin-binding-aptamers (15- and 29-mer) conjugated graphene oxide (TBA15/TBA29-GO) composite for the efficient inhibition of thrombin activity towards the formation of fibrin from fibrinogen.
29808843	2	35	theme	efficient	431:439	arg1	inhibition					441:450	the efficient inhibition	427:450	the efficient inhibition of thrombin activity towards the formation of fibrin from fibrinogen	427:519	In this study, we developed a thrombin-binding-aptamers (15- and 29-mer) conjugated graphene oxide (TBA15/TBA29-GO) composite for the efficient inhibition of thrombin activity towards the formation of fibrin from fibrinogen.
29808843	4	36	theme	binding	749:755	arg1	affinity					757:764	an ultrastrong binding affinity	734:764	an ultrastrong binding affinity for thrombin (dissociation constant = 2.9 × 10-12 M)	734:817	The high density and appropriate orientation of TBA15/TBA29 on the GO surface enabled TBA15/TBA29-GO to acquire an ultrastrong binding affinity for thrombin (dissociation constant = 2.9 × 10-12 M).
29808843	8	37	dep	hemostasis	1478:1487	arg1	time					1489:1492	time	1489:1492	time	1489:1492	The rat-tail bleeding assay of the hemostasis time and ex vivo PT and aPTT further revealed that TBA15/TBA29-GO is superior (>2-fold) to heparin, which is commonly used in the treatment and prevention of thrombotic diseases.
29808843	0	38	theme	bivalent	16:23	arg1	aptamers					25:32	bivalent aptamers	16:32	bivalent aptamers on graphene oxide as an efficient anticoagulant	16:80	Self-assembled, bivalent aptamers on graphene oxide as an efficient anticoagulant.
29808843	1	39	theme	biotechnology	269:281	arg1	applications					283:294	many biotechnology applications	264:294	many biotechnology applications	264:294	Graphene oxide (GO) has unique structural properties, can effectively adsorb single-strand DNA through π-π stacking, hydrogen bonding and hydrophobic interactions, and is useful in many biotechnology applications.
29808843	6	40	theme	thromboplastin	1177:1190	arg1	time					1192:1195	activated partial thromboplastin time	1159:1195	activated partial thromboplastin time	1159:1195	In addition, the prolonged thrombin clotting time, prothrombin time (PT), and activated partial thromboplastin time (aPTT) of TBA15/TBA29-GO were at least 2 times longer than those of commercially available drugs (heparin, argatroban, hirudin, and warfarin).
29808843	2	41	theme	activity	464:471	arg1	inhibition					441:450	the efficient inhibition	427:450	the efficient inhibition of thrombin activity towards the formation of fibrin from fibrinogen	427:519	In this study, we developed a thrombin-binding-aptamers (15- and 29-mer) conjugated graphene oxide (TBA15/TBA29-GO) composite for the efficient inhibition of thrombin activity towards the formation of fibrin from fibrinogen.
29808843	5	42	theme	higher	1043:1048	arg1	affinity					1058:1065	a higher binding affinity	1041:1065	a higher binding affinity for thrombin	1041:1078	Compared to bivalent TBA15h20A20/TBA29h20A20 hybrids, the TBA15/TBA29-GO composite exhibited a superior anticoagulant potency (ca. 10-fold) against thrombin-mediated coagulation as a result of steric blocking effects and a higher binding affinity for thrombin.
29808843	9	43	theme	effective	1823:1831	arg1	anticoagulants					1833:1846	effective anticoagulants	1823:1846	effective anticoagulants	1823:1846	Our multivalent, oligonucleotide-modified GO nanocomposites are easy to prepare, cost-effective, and highly biocompatible and they show great potential as effective anticoagulants for the treatment of thrombotic disorders.
29808843	5	44	theme	affinity	1058:1065	arg1	result					1003:1008	a result	1001:1008	a result of steric blocking effects and a higher binding affinity for thrombin	1001:1078	Compared to bivalent TBA15h20A20/TBA29h20A20 hybrids, the TBA15/TBA29-GO composite exhibited a superior anticoagulant potency (ca. 10-fold) against thrombin-mediated coagulation as a result of steric blocking effects and a higher binding affinity for thrombin.
29808843	5	44	theme	affinity	1058:1065	arg1	potency					938:944	a superior anticoagulant potency	913:944	a superior anticoagulant potency (ca. 10-fold) against thrombin-mediated coagulation	913:996	Compared to bivalent TBA15h20A20/TBA29h20A20 hybrids, the TBA15/TBA29-GO composite exhibited a superior anticoagulant potency (ca. 10-fold) against thrombin-mediated coagulation as a result of steric blocking effects and a higher binding affinity for thrombin.
29808843	5	45	theme	blocking	1020:1027	arg1	effects					1029:1035	steric blocking effects	1013:1035	steric blocking effects	1013:1035	Compared to bivalent TBA15h20A20/TBA29h20A20 hybrids, the TBA15/TBA29-GO composite exhibited a superior anticoagulant potency (ca. 10-fold) against thrombin-mediated coagulation as a result of steric blocking effects and a higher binding affinity for thrombin.
29808843	6	46	theme	prothrombin	1132:1142	arg1	time					1144:1147	prothrombin time	1132:1147	prothrombin time (PT)	1132:1152	In addition, the prolonged thrombin clotting time, prothrombin time (PT), and activated partial thromboplastin time (aPTT) of TBA15/TBA29-GO were at least 2 times longer than those of commercially available drugs (heparin, argatroban, hirudin, and warfarin).
29808843	6	46	theme	prothrombin	1132:1142	arg1	PT					1150:1151	PT	1150:1151	PT	1150:1151	In addition, the prolonged thrombin clotting time, prothrombin time (PT), and activated partial thromboplastin time (aPTT) of TBA15/TBA29-GO were at least 2 times longer than those of commercially available drugs (heparin, argatroban, hirudin, and warfarin).
29808843	1	47	theme	hydrophobic	221:231	arg1	interactions					233:244	hydrophobic interactions	221:244	hydrophobic interactions	221:244	Graphene oxide (GO) has unique structural properties, can effectively adsorb single-strand DNA through π-π stacking, hydrogen bonding and hydrophobic interactions, and is useful in many biotechnology applications.
29808843	6	48	dep	times	1238:1242	arg1	longer					1244:1249	longer	1244:1249	longer	1244:1249	In addition, the prolonged thrombin clotting time, prothrombin time (PT), and activated partial thromboplastin time (aPTT) of TBA15/TBA29-GO were at least 2 times longer than those of commercially available drugs (heparin, argatroban, hirudin, and warfarin).
29808843	9	49	theme	thrombotic	1869:1878	arg1	disorders					1880:1888	thrombotic disorders	1869:1888	thrombotic disorders	1869:1888	Our multivalent, oligonucleotide-modified GO nanocomposites are easy to prepare, cost-effective, and highly biocompatible and they show great potential as effective anticoagulants for the treatment of thrombotic disorders.
29808843	6	50	theme	thrombin	1108:1115	arg1	time					1126:1129	the prolonged thrombin clotting time	1094:1129	the prolonged thrombin clotting time	1094:1129	In addition, the prolonged thrombin clotting time, prothrombin time (PT), and activated partial thromboplastin time (aPTT) of TBA15/TBA29-GO were at least 2 times longer than those of commercially available drugs (heparin, argatroban, hirudin, and warfarin).
29808843	6	50	theme	thrombin	1108:1115	arg1	aPTT					1198:1201	aPTT	1198:1201	aPTT	1198:1201	In addition, the prolonged thrombin clotting time, prothrombin time (PT), and activated partial thromboplastin time (aPTT) of TBA15/TBA29-GO were at least 2 times longer than those of commercially available drugs (heparin, argatroban, hirudin, and warfarin).
29808843	6	50	theme	thrombin	1108:1115	arg1	times					1238:1242	times	1238:1242	times	1238:1242	In addition, the prolonged thrombin clotting time, prothrombin time (PT), and activated partial thromboplastin time (aPTT) of TBA15/TBA29-GO were at least 2 times longer than those of commercially available drugs (heparin, argatroban, hirudin, and warfarin).
29808843	4	51	dep	density	631:637	arg1	The					622:624	The	622:624	The	622:624	The high density and appropriate orientation of TBA15/TBA29 on the GO surface enabled TBA15/TBA29-GO to acquire an ultrastrong binding affinity for thrombin (dissociation constant = 2.9 × 10-12 M).
29808843	8	52	theme	diseases	1658:1665	arg1	prevention					1633:1642	prevention	1633:1642	prevention	1633:1642	The rat-tail bleeding assay of the hemostasis time and ex vivo PT and aPTT further revealed that TBA15/TBA29-GO is superior (>2-fold) to heparin, which is commonly used in the treatment and prevention of thrombotic diseases.
29808843	8	52	theme	diseases	1658:1665	arg1	treatment					1619:1627	treatment	1619:1627	treatment	1619:1627	The rat-tail bleeding assay of the hemostasis time and ex vivo PT and aPTT further revealed that TBA15/TBA29-GO is superior (>2-fold) to heparin, which is commonly used in the treatment and prevention of thrombotic diseases.
29808843	7	53	theme	TBA15/TBA29-GO	1427:1440	arg1	biocompatibility					1407:1422	the high biocompatibility	1398:1422	the high biocompatibility of TBA15/TBA29-GO	1398:1440	The in vitro cytotoxicity and hemolysis analyses revealed the high biocompatibility of TBA15/TBA29-GO.
29808843	7	54	dep	in	1344:1345	arg1	vitro					1347:1351	vitro	1347:1351	vitro	1347:1351	The in vitro cytotoxicity and hemolysis analyses revealed the high biocompatibility of TBA15/TBA29-GO.
29808843	6	55	theme	partial	1169:1175	arg1	time					1192:1195	activated partial thromboplastin time	1159:1195	activated partial thromboplastin time	1159:1195	In addition, the prolonged thrombin clotting time, prothrombin time (PT), and activated partial thromboplastin time (aPTT) of TBA15/TBA29-GO were at least 2 times longer than those of commercially available drugs (heparin, argatroban, hirudin, and warfarin).
29808843	4	56	from	orientation	655:665	arg1	surface					692:698	the GO surface	685:698	the GO surface	685:698	The high density and appropriate orientation of TBA15/TBA29 on the GO surface enabled TBA15/TBA29-GO to acquire an ultrastrong binding affinity for thrombin (dissociation constant = 2.9 × 10-12 M).
29808843	5	57	theme	TBA15/TBA29-GO	878:891	arg1	composite					893:901	the TBA15/TBA29-GO composite	874:901	the TBA15/TBA29-GO composite	874:901	Compared to bivalent TBA15h20A20/TBA29h20A20 hybrids, the TBA15/TBA29-GO composite exhibited a superior anticoagulant potency (ca. 10-fold) against thrombin-mediated coagulation as a result of steric blocking effects and a higher binding affinity for thrombin.
29808843	4	58	theme	TBA15/TBA29	670:680	arg1	density					631:637	high density	626:637	high density	626:637	The high density and appropriate orientation of TBA15/TBA29 on the GO surface enabled TBA15/TBA29-GO to acquire an ultrastrong binding affinity for thrombin (dissociation constant = 2.9 × 10-12 M).
29808843	4	58	theme	TBA15/TBA29	670:680	arg1	orientation					655:665	appropriate orientation	643:665	appropriate orientation	643:665	The high density and appropriate orientation of TBA15/TBA29 on the GO surface enabled TBA15/TBA29-GO to acquire an ultrastrong binding affinity for thrombin (dissociation constant = 2.9 × 10-12 M).
29808843	1	59	theme	π-π	186:188	arg1	stacking					190:197	π-π stacking	186:197	π-π stacking	186:197	Graphene oxide (GO) has unique structural properties, can effectively adsorb single-strand DNA through π-π stacking, hydrogen bonding and hydrophobic interactions, and is useful in many biotechnology applications.
29808843	9	60	theme	multivalent	1672:1682	arg1	nanocomposites					1713:1726	Our multivalent, oligonucleotide-modified GO nanocomposites	1668:1726	Our multivalent, oligonucleotide-modified GO nanocomposites	1668:1726	Our multivalent, oligonucleotide-modified GO nanocomposites are easy to prepare, cost-effective, and highly biocompatible and they show great potential as effective anticoagulants for the treatment of thrombotic disorders.
29808843	4	61	dep	thrombin	770:777	arg1	constant					793:800	dissociation constant	780:800	dissociation constant = 2.9 × 10-12 M	780:816	The high density and appropriate orientation of TBA15/TBA29 on the GO surface enabled TBA15/TBA29-GO to acquire an ultrastrong binding affinity for thrombin (dissociation constant = 2.9 × 10-12 M).
29808843	4	62	theme	high	626:629	arg1	density					631:637	high density	626:637	high density	626:637	The high density and appropriate orientation of TBA15/TBA29 on the GO surface enabled TBA15/TBA29-GO to acquire an ultrastrong binding affinity for thrombin (dissociation constant = 2.9 × 10-12 M).
29808843	3	63	theme	TBA15/TBA29	595:605	arg1	hybrids					607:613	TBA15/TBA29 hybrids	595:613	TBA15/TBA29 hybrids	595:613	The TBA15/TBA29-GO composite was simply obtained by the self-assembly of TBA15/TBA29 hybrids on GO.
29808843	5	64	theme	TBA15h20A20/TBA29h20A20	841:863	arg1	hybrids					865:871	bivalent TBA15h20A20/TBA29h20A20 hybrids	832:871	bivalent TBA15h20A20/TBA29h20A20 hybrids	832:871	Compared to bivalent TBA15h20A20/TBA29h20A20 hybrids, the TBA15/TBA29-GO composite exhibited a superior anticoagulant potency (ca. 10-fold) against thrombin-mediated coagulation as a result of steric blocking effects and a higher binding affinity for thrombin.
29808843	8	65	theme	bleeding	1456:1463	arg1	assay					1465:1469	The rat-tail bleeding assay	1443:1469	The rat-tail bleeding assay of the hemostasis time and ex vivo PT and aPTT	1443:1516	The rat-tail bleeding assay of the hemostasis time and ex vivo PT and aPTT further revealed that TBA15/TBA29-GO is superior (>2-fold) to heparin, which is commonly used in the treatment and prevention of thrombotic diseases.
29808843	8	66	dep	superior	1558:1565	arg1	>2-fold					1568:1574	>2-fold	1568:1574	>2-fold	1568:1574	The rat-tail bleeding assay of the hemostasis time and ex vivo PT and aPTT further revealed that TBA15/TBA29-GO is superior (>2-fold) to heparin, which is commonly used in the treatment and prevention of thrombotic diseases.
29808843	1	67	theme	unique	107:112	arg1	properties					125:134	unique structural properties	107:134	unique structural properties	107:134	Graphene oxide (GO) has unique structural properties, can effectively adsorb single-strand DNA through π-π stacking, hydrogen bonding and hydrophobic interactions, and is useful in many biotechnology applications.
29808843	8	68	theme	hemostasis	1478:1487	arg1	assay					1465:1469	The rat-tail bleeding assay	1443:1469	The rat-tail bleeding assay of the hemostasis time and ex vivo PT and aPTT	1443:1516	The rat-tail bleeding assay of the hemostasis time and ex vivo PT and aPTT further revealed that TBA15/TBA29-GO is superior (>2-fold) to heparin, which is commonly used in the treatment and prevention of thrombotic diseases.
29808843	0	69	theme	efficient	58:66	arg1	anticoagulant					68:80	an efficient anticoagulant	55:80	an efficient anticoagulant	55:80	Self-assembled, bivalent aptamers on graphene oxide as an efficient anticoagulant.
29808843	0	70	from	aptamers	25:32	arg1	oxide					46:50	graphene oxide	37:50	graphene oxide	37:50	Self-assembled, bivalent aptamers on graphene oxide as an efficient anticoagulant.
29808843	8	71	dep	treatment	1619:1627	arg1	the					1615:1617	the	1615:1617	the	1615:1617	The rat-tail bleeding assay of the hemostasis time and ex vivo PT and aPTT further revealed that TBA15/TBA29-GO is superior (>2-fold) to heparin, which is commonly used in the treatment and prevention of thrombotic diseases.
29808843	5	72	theme	superior	915:922	arg1	result					1003:1008	a result	1001:1008	a result of steric blocking effects and a higher binding affinity for thrombin	1001:1078	Compared to bivalent TBA15h20A20/TBA29h20A20 hybrids, the TBA15/TBA29-GO composite exhibited a superior anticoagulant potency (ca. 10-fold) against thrombin-mediated coagulation as a result of steric blocking effects and a higher binding affinity for thrombin.
29808843	5	72	theme	superior	915:922	arg1	potency					938:944	a superior anticoagulant potency	913:944	a superior anticoagulant potency (ca. 10-fold) against thrombin-mediated coagulation	913:996	Compared to bivalent TBA15h20A20/TBA29h20A20 hybrids, the TBA15/TBA29-GO composite exhibited a superior anticoagulant potency (ca. 10-fold) against thrombin-mediated coagulation as a result of steric blocking effects and a higher binding affinity for thrombin.
29808843	1	73	dep	oxide	92:96	arg1	GO					99:100	GO	99:100	GO	99:100	Graphene oxide (GO) has unique structural properties, can effectively adsorb single-strand DNA through π-π stacking, hydrogen bonding and hydrophobic interactions, and is useful in many biotechnology applications.
29808843	2	74	dep	thrombin-binding-aptamers	327:351	arg1	29-mer					362:367	29-mer	362:367	29-mer	362:367	In this study, we developed a thrombin-binding-aptamers (15- and 29-mer) conjugated graphene oxide (TBA15/TBA29-GO) composite for the efficient inhibition of thrombin activity towards the formation of fibrin from fibrinogen.
29808843	2	74	dep	thrombin-binding-aptamers	327:351	arg1	15-					354:356	15-	354:356	15-	354:356	In this study, we developed a thrombin-binding-aptamers (15- and 29-mer) conjugated graphene oxide (TBA15/TBA29-GO) composite for the efficient inhibition of thrombin activity towards the formation of fibrin from fibrinogen.
29808843	5	75	dep	10-fold	951:957	arg1	ca.					947:949	ca.	947:949	ca.	947:949	Compared to bivalent TBA15h20A20/TBA29h20A20 hybrids, the TBA15/TBA29-GO composite exhibited a superior anticoagulant potency (ca. 10-fold) against thrombin-mediated coagulation as a result of steric blocking effects and a higher binding affinity for thrombin.
29808843	6	76	theme	prolonged	1098:1106	arg1	time					1126:1129	the prolonged thrombin clotting time	1094:1129	the prolonged thrombin clotting time	1094:1129	In addition, the prolonged thrombin clotting time, prothrombin time (PT), and activated partial thromboplastin time (aPTT) of TBA15/TBA29-GO were at least 2 times longer than those of commercially available drugs (heparin, argatroban, hirudin, and warfarin).
29808843	6	76	theme	prolonged	1098:1106	arg1	aPTT					1198:1201	aPTT	1198:1201	aPTT	1198:1201	In addition, the prolonged thrombin clotting time, prothrombin time (PT), and activated partial thromboplastin time (aPTT) of TBA15/TBA29-GO were at least 2 times longer than those of commercially available drugs (heparin, argatroban, hirudin, and warfarin).
29808843	6	76	theme	prolonged	1098:1106	arg1	times					1238:1242	times	1238:1242	times	1238:1242	In addition, the prolonged thrombin clotting time, prothrombin time (PT), and activated partial thromboplastin time (aPTT) of TBA15/TBA29-GO were at least 2 times longer than those of commercially available drugs (heparin, argatroban, hirudin, and warfarin).
29808843	2	77	theme	fibrin	498:503	arg1	formation					485:493	the formation	481:493	the formation of fibrin from fibrinogen	481:519	In this study, we developed a thrombin-binding-aptamers (15- and 29-mer) conjugated graphene oxide (TBA15/TBA29-GO) composite for the efficient inhibition of thrombin activity towards the formation of fibrin from fibrinogen.
29808843	4	78	theme	dissociation	780:791	arg1	constant					793:800	dissociation constant	780:800	dissociation constant = 2.9 × 10-12 M	780:816	The high density and appropriate orientation of TBA15/TBA29 on the GO surface enabled TBA15/TBA29-GO to acquire an ultrastrong binding affinity for thrombin (dissociation constant = 2.9 × 10-12 M).
29808843	1	79	theme	Graphene	83:90	arg1	oxide					92:96	Graphene oxide	83:96	Graphene oxide (GO)	83:101	Graphene oxide (GO) has unique structural properties, can effectively adsorb single-strand DNA through π-π stacking, hydrogen bonding and hydrophobic interactions, and is useful in many biotechnology applications.
29808843	4	80	theme	ultrastrong	737:747	arg1	affinity					757:764	an ultrastrong binding affinity	734:764	an ultrastrong binding affinity for thrombin (dissociation constant = 2.9 × 10-12 M)	734:817	The high density and appropriate orientation of TBA15/TBA29 on the GO surface enabled TBA15/TBA29-GO to acquire an ultrastrong binding affinity for thrombin (dissociation constant = 2.9 × 10-12 M).
29808843	3	81	from	self-assembly	578:590	arg1	GO					618:619	GO	618:619	GO	618:619	The TBA15/TBA29-GO composite was simply obtained by the self-assembly of TBA15/TBA29 hybrids on GO.
29808843	1	82	theme	many	264:267	arg1	applications					283:294	many biotechnology applications	264:294	many biotechnology applications	264:294	Graphene oxide (GO) has unique structural properties, can effectively adsorb single-strand DNA through π-π stacking, hydrogen bonding and hydrophobic interactions, and is useful in many biotechnology applications.
29808843	5	83	theme	thrombin-mediated	968:984	arg1	coagulation					986:996	thrombin-mediated coagulation	968:996	thrombin-mediated coagulation	968:996	Compared to bivalent TBA15h20A20/TBA29h20A20 hybrids, the TBA15/TBA29-GO composite exhibited a superior anticoagulant potency (ca. 10-fold) against thrombin-mediated coagulation as a result of steric blocking effects and a higher binding affinity for thrombin.
29808843	7	84	theme	cytotoxicity	1353:1364	arg1	analyses					1380:1387	The in vitro cytotoxicity and hemolysis analyses	1340:1387	The in vitro cytotoxicity and hemolysis analyses	1340:1387	The in vitro cytotoxicity and hemolysis analyses revealed the high biocompatibility of TBA15/TBA29-GO.
29808843	9	85	theme	great	1804:1808	arg1	potential					1810:1818	great potential	1804:1818	great potential as effective anticoagulants for the treatment of thrombotic disorders	1804:1888	Our multivalent, oligonucleotide-modified GO nanocomposites are easy to prepare, cost-effective, and highly biocompatible and they show great potential as effective anticoagulants for the treatment of thrombotic disorders.
29808843	0	86	theme	graphene	37:44	arg1	oxide					46:50	graphene oxide	37:50	graphene oxide	37:50	Self-assembled, bivalent aptamers on graphene oxide as an efficient anticoagulant.
29808843	2	87	theme	thrombin	455:462	arg1	activity					464:471	thrombin activity	455:471	thrombin activity towards the formation of fibrin from fibrinogen	455:519	In this study, we developed a thrombin-binding-aptamers (15- and 29-mer) conjugated graphene oxide (TBA15/TBA29-GO) composite for the efficient inhibition of thrombin activity towards the formation of fibrin from fibrinogen.
29808843	5	88	theme	binding	1050:1056	arg1	affinity					1058:1065	a higher binding affinity	1041:1065	a higher binding affinity for thrombin	1041:1078	Compared to bivalent TBA15h20A20/TBA29h20A20 hybrids, the TBA15/TBA29-GO composite exhibited a superior anticoagulant potency (ca. 10-fold) against thrombin-mediated coagulation as a result of steric blocking effects and a higher binding affinity for thrombin.
29808843	5	89	theme	steric	1013:1018	arg1	effects					1029:1035	steric blocking effects	1013:1035	steric blocking effects	1013:1035	Compared to bivalent TBA15h20A20/TBA29h20A20 hybrids, the TBA15/TBA29-GO composite exhibited a superior anticoagulant potency (ca. 10-fold) against thrombin-mediated coagulation as a result of steric blocking effects and a higher binding affinity for thrombin.
29808843	4	90	theme	=	802:802	arg1	constant					793:800	dissociation constant	780:800	dissociation constant = 2.9 × 10-12 M	780:816	The high density and appropriate orientation of TBA15/TBA29 on the GO surface enabled TBA15/TBA29-GO to acquire an ultrastrong binding affinity for thrombin (dissociation constant = 2.9 × 10-12 M).
29808843	5	91	theme	effects	1029:1035	arg1	result					1003:1008	a result	1001:1008	a result of steric blocking effects and a higher binding affinity for thrombin	1001:1078	Compared to bivalent TBA15h20A20/TBA29h20A20 hybrids, the TBA15/TBA29-GO composite exhibited a superior anticoagulant potency (ca. 10-fold) against thrombin-mediated coagulation as a result of steric blocking effects and a higher binding affinity for thrombin.
29808843	5	91	theme	effects	1029:1035	arg1	potency					938:944	a superior anticoagulant potency	913:944	a superior anticoagulant potency (ca. 10-fold) against thrombin-mediated coagulation	913:996	Compared to bivalent TBA15h20A20/TBA29h20A20 hybrids, the TBA15/TBA29-GO composite exhibited a superior anticoagulant potency (ca. 10-fold) against thrombin-mediated coagulation as a result of steric blocking effects and a higher binding affinity for thrombin.
29808843	8	92	theme	thrombotic	1647:1656	arg1	diseases					1658:1665	thrombotic diseases	1647:1665	thrombotic diseases	1647:1665	The rat-tail bleeding assay of the hemostasis time and ex vivo PT and aPTT further revealed that TBA15/TBA29-GO is superior (>2-fold) to heparin, which is commonly used in the treatment and prevention of thrombotic diseases.
30678906	8	0	theme	external	1572:1579	arg1	fields					1590:1595	strong external magnetic fields	1565:1595	strong external magnetic fields	1565:1595	In addition, the saturation magnetization, coercive force, and remanence to saturation magnetization ratio of DOX-FA-CS-CNT(Fe)/HA were 0.88 emu g-1, 668.96 Oe, and 0.44, respectively, indicating its potential for drug transport under strong external magnetic fields, which could enable magnetic targeted delivery.
30678906	1	1	theme	as-fabricated	378:390	arg1	/HA					399:401	the as-fabricated CNT(Fe)/HA	374:401	the as-fabricated CNT(Fe)/HA	374:401	A novel magnetic targeted drug delivery carrier based on a carbon nanotube (Fe)/hydroxyapatite (CNT(Fe)/HA) composite was successfully fabricated by an in situ synthesis of CNTs in HA nanopowder using Fe catalysts and subsequent chemical modification of the as-fabricated CNT(Fe)/HA by chitosan (CS) and folic acid (FA) for a controllable release of an anticancer drug doxorubicin (DOX).
30678906	6	2	theme	release	1230:1236	arg1	rate					1238:1241	an average drug release rate	1214:1241	an average drug release rate of 52 wt% after 72 h	1214:1262	Furthermore, the pH response of FA-CS-CNT(Fe)/HA enabled the release of a large amount of DOX in phosphate-buffered saline (PBS) at pH = 5.5 with an average drug release rate of 52 wt% after 72 h.
30678906	1	3	theme	Fe	220:221	arg1	composite					228:236	a carbon nanotube (Fe)/hydroxyapatite (CNT(Fe)/HA) composite	177:236	a carbon nanotube (Fe)/hydroxyapatite (CNT(Fe)/HA) composite	177:236	A novel magnetic targeted drug delivery carrier based on a carbon nanotube (Fe)/hydroxyapatite (CNT(Fe)/HA) composite was successfully fabricated by an in situ synthesis of CNTs in HA nanopowder using Fe catalysts and subsequent chemical modification of the as-fabricated CNT(Fe)/HA by chitosan (CS) and folic acid (FA) for a controllable release of an anticancer drug doxorubicin (DOX).
30678906	8	4	theme	coercive	1373:1380	arg1	force					1382:1386	coercive force	1373:1386	coercive force	1373:1386	In addition, the saturation magnetization, coercive force, and remanence to saturation magnetization ratio of DOX-FA-CS-CNT(Fe)/HA were 0.88 emu g-1, 668.96 Oe, and 0.44, respectively, indicating its potential for drug transport under strong external magnetic fields, which could enable magnetic targeted delivery.
30678906	6	5	theme	pH	1085:1086	arg1	response					1088:1095	the pH response	1081:1095	the pH response of FA-CS-CNT(Fe)/HA	1081:1115	Furthermore, the pH response of FA-CS-CNT(Fe)/HA enabled the release of a large amount of DOX in phosphate-buffered saline (PBS) at pH = 5.5 with an average drug release rate of 52 wt% after 72 h.
30678906	8	6	theme	DOX-FA-CS-CNT	1440:1452	arg1	/HA					1457:1459	DOX-FA-CS-CNT(Fe)/HA	1440:1459	DOX-FA-CS-CNT(Fe)/HA	1440:1459	In addition, the saturation magnetization, coercive force, and remanence to saturation magnetization ratio of DOX-FA-CS-CNT(Fe)/HA were 0.88 emu g-1, 668.96 Oe, and 0.44, respectively, indicating its potential for drug transport under strong external magnetic fields, which could enable magnetic targeted delivery.
30678906	1	7	theme	/HA	223:225	arg1	composite					228:236	a carbon nanotube (Fe)/hydroxyapatite (CNT(Fe)/HA) composite	177:236	a carbon nanotube (Fe)/hydroxyapatite (CNT(Fe)/HA) composite	177:236	A novel magnetic targeted drug delivery carrier based on a carbon nanotube (Fe)/hydroxyapatite (CNT(Fe)/HA) composite was successfully fabricated by an in situ synthesis of CNTs in HA nanopowder using Fe catalysts and subsequent chemical modification of the as-fabricated CNT(Fe)/HA by chitosan (CS) and folic acid (FA) for a controllable release of an anticancer drug doxorubicin (DOX).
30678906	7	8	from	release	1287:1293	arg1	PBS					1298:1300	PBS	1298:1300	PBS	1298:1300	In contrast, the drug release in PBS at pH = 7.4 was only 8 wt%.
30678906	2	9	theme	synthesized	512:522	arg1	CNTs					524:527	The synthesized CNTs	508:527	The synthesized CNTs	508:527	The synthesized CNTs, Fe, and HA self-assembled into a composite structure in situ during the synthesis.
30678906	8	10	theme	Fe	1454:1455	arg1	/HA					1457:1459	DOX-FA-CS-CNT(Fe)/HA	1440:1459	DOX-FA-CS-CNT(Fe)/HA	1440:1459	In addition, the saturation magnetization, coercive force, and remanence to saturation magnetization ratio of DOX-FA-CS-CNT(Fe)/HA were 0.88 emu g-1, 668.96 Oe, and 0.44, respectively, indicating its potential for drug transport under strong external magnetic fields, which could enable magnetic targeted delivery.
30678906	4	11	theme	electrostatic	962:974	arg1	adsorption					976:985	electrostatic adsorption	962:985	electrostatic adsorption	962:985	Upon the functional modification through the surface coating with CS and FA, the functionalized CNT(Fe)/HA became capable of loading DOX through both π-π stacking and electrostatic adsorption of FA.
30678906	8	12	theme	/HA	1457:1459	arg1	magnetization					1358:1370	the saturation magnetization	1343:1370	the saturation magnetization	1343:1370	In addition, the saturation magnetization, coercive force, and remanence to saturation magnetization ratio of DOX-FA-CS-CNT(Fe)/HA were 0.88 emu g-1, 668.96 Oe, and 0.44, respectively, indicating its potential for drug transport under strong external magnetic fields, which could enable magnetic targeted delivery.
30678906	8	12	theme	/HA	1457:1459	arg1	force					1382:1386	coercive force	1373:1386	coercive force	1373:1386	In addition, the saturation magnetization, coercive force, and remanence to saturation magnetization ratio of DOX-FA-CS-CNT(Fe)/HA were 0.88 emu g-1, 668.96 Oe, and 0.44, respectively, indicating its potential for drug transport under strong external magnetic fields, which could enable magnetic targeted delivery.
30678906	8	12	theme	/HA	1457:1459	arg1	0.88 emu g-1					1466:1477	0.88 emu g-1	1466:1477	0.88 emu g-1	1466:1477	In addition, the saturation magnetization, coercive force, and remanence to saturation magnetization ratio of DOX-FA-CS-CNT(Fe)/HA were 0.88 emu g-1, 668.96 Oe, and 0.44, respectively, indicating its potential for drug transport under strong external magnetic fields, which could enable magnetic targeted delivery.
30678906	8	12	theme	/HA	1457:1459	arg1	ratio					1431:1435	remanence to saturation magnetization ratio	1393:1435	remanence to saturation magnetization ratio of DOX-FA-CS-CNT(Fe)/HA	1393:1459	In addition, the saturation magnetization, coercive force, and remanence to saturation magnetization ratio of DOX-FA-CS-CNT(Fe)/HA were 0.88 emu g-1, 668.96 Oe, and 0.44, respectively, indicating its potential for drug transport under strong external magnetic fields, which could enable magnetic targeted delivery.
30678906	4	13	theme	DOX	928:930	arg1	capable					909:915	capable	909:915	capable	909:915	Upon the functional modification through the surface coating with CS and FA, the functionalized CNT(Fe)/HA became capable of loading DOX through both π-π stacking and electrostatic adsorption of FA.
30678906	8	14	theme	targeted	1626:1633	arg1	delivery					1635:1642	magnetic targeted delivery	1617:1642	magnetic targeted delivery	1617:1642	In addition, the saturation magnetization, coercive force, and remanence to saturation magnetization ratio of DOX-FA-CS-CNT(Fe)/HA were 0.88 emu g-1, 668.96 Oe, and 0.44, respectively, indicating its potential for drug transport under strong external magnetic fields, which could enable magnetic targeted delivery.
30678906	1	15	theme	Fe	321:322	arg1	catalysts					324:332	Fe catalysts	321:332	Fe catalysts	321:332	A novel magnetic targeted drug delivery carrier based on a carbon nanotube (Fe)/hydroxyapatite (CNT(Fe)/HA) composite was successfully fabricated by an in situ synthesis of CNTs in HA nanopowder using Fe catalysts and subsequent chemical modification of the as-fabricated CNT(Fe)/HA by chitosan (CS) and folic acid (FA) for a controllable release of an anticancer drug doxorubicin (DOX).
30678906	1	16	theme	nanotube	186:193	arg1	composite					228:236	a carbon nanotube (Fe)/hydroxyapatite (CNT(Fe)/HA) composite	177:236	a carbon nanotube (Fe)/hydroxyapatite (CNT(Fe)/HA) composite	177:236	A novel magnetic targeted drug delivery carrier based on a carbon nanotube (Fe)/hydroxyapatite (CNT(Fe)/HA) composite was successfully fabricated by an in situ synthesis of CNTs in HA nanopowder using Fe catalysts and subsequent chemical modification of the as-fabricated CNT(Fe)/HA by chitosan (CS) and folic acid (FA) for a controllable release of an anticancer drug doxorubicin (DOX).
30678906	5	17	theme	drug-loading	1030:1041	arg1	rate					1043:1046	the average drug-loading rate	1018:1046	the average drug-loading rate of DOX	1018:1053	The results showed that the average drug-loading rate of DOX was 130 wt%.
30678906	5	17	theme	drug-loading	1030:1041	arg1	%					1065:1065	130 wt%	1059:1065	130 wt%	1059:1065	The results showed that the average drug-loading rate of DOX was 130 wt%.
30678906	1	18	theme	Fe	196:197	arg1	composite					228:236	a carbon nanotube (Fe)/hydroxyapatite (CNT(Fe)/HA) composite	177:236	a carbon nanotube (Fe)/hydroxyapatite (CNT(Fe)/HA) composite	177:236	A novel magnetic targeted drug delivery carrier based on a carbon nanotube (Fe)/hydroxyapatite (CNT(Fe)/HA) composite was successfully fabricated by an in situ synthesis of CNTs in HA nanopowder using Fe catalysts and subsequent chemical modification of the as-fabricated CNT(Fe)/HA by chitosan (CS) and folic acid (FA) for a controllable release of an anticancer drug doxorubicin (DOX).
30678906	1	19	theme	chemical	349:356	arg1	modification					358:369	subsequent chemical modification	338:369	subsequent chemical modification of the as-fabricated CNT(Fe)/HA by chitosan (CS) and folic acid (FA) for a controllable release of an anticancer drug doxorubicin (DOX)	338:505	A novel magnetic targeted drug delivery carrier based on a carbon nanotube (Fe)/hydroxyapatite (CNT(Fe)/HA) composite was successfully fabricated by an in situ synthesis of CNTs in HA nanopowder using Fe catalysts and subsequent chemical modification of the as-fabricated CNT(Fe)/HA by chitosan (CS) and folic acid (FA) for a controllable release of an anticancer drug doxorubicin (DOX).
30678906	3	20	theme	CNTs	706:709	arg1	tips					694:697	the tips	690:697	the tips of the CNTs	690:709	After the acid treatment, the CNTs were shorter and homogeneously dispersed, the tips of the CNTs were opened, and oxygen-containing functional groups were introduced onto the CNTs.
30678906	1	21	theme	doxorubicin	489:499	arg1	release					459:465	a controllable release	444:465	a controllable release of an anticancer drug doxorubicin (DOX)	444:505	A novel magnetic targeted drug delivery carrier based on a carbon nanotube (Fe)/hydroxyapatite (CNT(Fe)/HA) composite was successfully fabricated by an in situ synthesis of CNTs in HA nanopowder using Fe catalysts and subsequent chemical modification of the as-fabricated CNT(Fe)/HA by chitosan (CS) and folic acid (FA) for a controllable release of an anticancer drug doxorubicin (DOX).
30678906	1	22	theme	novel	122:126	arg1	carrier					160:166	A novel magnetic targeted drug delivery carrier	120:166	A novel magnetic targeted drug delivery carrier based on a carbon nanotube (Fe)/hydroxyapatite (CNT(Fe)/HA) composite	120:236	A novel magnetic targeted drug delivery carrier based on a carbon nanotube (Fe)/hydroxyapatite (CNT(Fe)/HA) composite was successfully fabricated by an in situ synthesis of CNTs in HA nanopowder using Fe catalysts and subsequent chemical modification of the as-fabricated CNT(Fe)/HA by chitosan (CS) and folic acid (FA) for a controllable release of an anticancer drug doxorubicin (DOX).
30678906	8	23	theme	magnetization	1417:1429	arg1	ratio					1431:1435	remanence to saturation magnetization ratio	1393:1435	remanence to saturation magnetization ratio of DOX-FA-CS-CNT(Fe)/HA	1393:1459	In addition, the saturation magnetization, coercive force, and remanence to saturation magnetization ratio of DOX-FA-CS-CNT(Fe)/HA were 0.88 emu g-1, 668.96 Oe, and 0.44, respectively, indicating its potential for drug transport under strong external magnetic fields, which could enable magnetic targeted delivery.
30678906	6	24	theme	phosphate-buffered	1165:1182	arg1	PBS					1192:1194	PBS	1192:1194	PBS	1192:1194	Furthermore, the pH response of FA-CS-CNT(Fe)/HA enabled the release of a large amount of DOX in phosphate-buffered saline (PBS) at pH = 5.5 with an average drug release rate of 52 wt% after 72 h.
30678906	6	24	theme	phosphate-buffered	1165:1182	arg1	saline					1184:1189	phosphate-buffered saline	1165:1189	phosphate-buffered saline (PBS)	1165:1195	Furthermore, the pH response of FA-CS-CNT(Fe)/HA enabled the release of a large amount of DOX in phosphate-buffered saline (PBS) at pH = 5.5 with an average drug release rate of 52 wt% after 72 h.
30678906	1	25	theme	targeted	137:144	arg1	carrier					160:166	A novel magnetic targeted drug delivery carrier	120:166	A novel magnetic targeted drug delivery carrier based on a carbon nanotube (Fe)/hydroxyapatite (CNT(Fe)/HA) composite	120:236	A novel magnetic targeted drug delivery carrier based on a carbon nanotube (Fe)/hydroxyapatite (CNT(Fe)/HA) composite was successfully fabricated by an in situ synthesis of CNTs in HA nanopowder using Fe catalysts and subsequent chemical modification of the as-fabricated CNT(Fe)/HA by chitosan (CS) and folic acid (FA) for a controllable release of an anticancer drug doxorubicin (DOX).
30678906	6	26	theme	DOX	1158:1160	arg1	amount					1148:1153	a large amount	1140:1153	a large amount of DOX	1140:1160	Furthermore, the pH response of FA-CS-CNT(Fe)/HA enabled the release of a large amount of DOX in phosphate-buffered saline (PBS) at pH = 5.5 with an average drug release rate of 52 wt% after 72 h.
30678906	6	26	theme	DOX	1158:1160	arg1	DOX					1158:1160	DOX	1158:1160	DOX	1158:1160	Furthermore, the pH response of FA-CS-CNT(Fe)/HA enabled the release of a large amount of DOX in phosphate-buffered saline (PBS) at pH = 5.5 with an average drug release rate of 52 wt% after 72 h.
30678906	1	27	theme	delivery	151:158	arg1	carrier					160:166	A novel magnetic targeted drug delivery carrier	120:166	A novel magnetic targeted drug delivery carrier based on a carbon nanotube (Fe)/hydroxyapatite (CNT(Fe)/HA) composite	120:236	A novel magnetic targeted drug delivery carrier based on a carbon nanotube (Fe)/hydroxyapatite (CNT(Fe)/HA) composite was successfully fabricated by an in situ synthesis of CNTs in HA nanopowder using Fe catalysts and subsequent chemical modification of the as-fabricated CNT(Fe)/HA by chitosan (CS) and folic acid (FA) for a controllable release of an anticancer drug doxorubicin (DOX).
30678906	0	28	theme	magnetic	79:86	arg1	delivery					102:109	magnetic targeted drug delivery	79:109	magnetic targeted drug delivery carrier	79:117	Preparation and properties of carbon nanotube (Fe)/hydroxyapatite composite as magnetic targeted drug delivery carrier.
30678906	1	29	dep	in	272:273	arg1	situ					275:278	situ	275:278	situ	275:278	A novel magnetic targeted drug delivery carrier based on a carbon nanotube (Fe)/hydroxyapatite (CNT(Fe)/HA) composite was successfully fabricated by an in situ synthesis of CNTs in HA nanopowder using Fe catalysts and subsequent chemical modification of the as-fabricated CNT(Fe)/HA by chitosan (CS) and folic acid (FA) for a controllable release of an anticancer drug doxorubicin (DOX).
30678906	0	30	theme	drug	97:100	arg1	delivery					102:109	magnetic targeted drug delivery	79:109	magnetic targeted drug delivery carrier	79:117	Preparation and properties of carbon nanotube (Fe)/hydroxyapatite composite as magnetic targeted drug delivery carrier.
30678906	3	31	theme	functional	746:755	arg1	groups					757:762	oxygen-containing functional groups	728:762	oxygen-containing functional groups	728:762	After the acid treatment, the CNTs were shorter and homogeneously dispersed, the tips of the CNTs were opened, and oxygen-containing functional groups were introduced onto the CNTs.
30678906	1	32	theme	controllable	446:457	arg1	release					459:465	a controllable release	444:465	a controllable release of an anticancer drug doxorubicin (DOX)	444:505	A novel magnetic targeted drug delivery carrier based on a carbon nanotube (Fe)/hydroxyapatite (CNT(Fe)/HA) composite was successfully fabricated by an in situ synthesis of CNTs in HA nanopowder using Fe catalysts and subsequent chemical modification of the as-fabricated CNT(Fe)/HA by chitosan (CS) and folic acid (FA) for a controllable release of an anticancer drug doxorubicin (DOX).
30678906	8	33	theme	strong	1565:1570	arg1	fields					1590:1595	strong external magnetic fields	1565:1595	strong external magnetic fields	1565:1595	In addition, the saturation magnetization, coercive force, and remanence to saturation magnetization ratio of DOX-FA-CS-CNT(Fe)/HA were 0.88 emu g-1, 668.96 Oe, and 0.44, respectively, indicating its potential for drug transport under strong external magnetic fields, which could enable magnetic targeted delivery.
30678906	4	34	theme	CNT	891:893	arg1	/HA					898:900	the functionalized CNT(Fe)/HA	872:900	the functionalized CNT(Fe)/HA	872:900	Upon the functional modification through the surface coating with CS and FA, the functionalized CNT(Fe)/HA became capable of loading DOX through both π-π stacking and electrostatic adsorption of FA.
30678906	1	35	theme	anticancer	473:482	arg1	DOX					502:504	DOX	502:504	DOX	502:504	A novel magnetic targeted drug delivery carrier based on a carbon nanotube (Fe)/hydroxyapatite (CNT(Fe)/HA) composite was successfully fabricated by an in situ synthesis of CNTs in HA nanopowder using Fe catalysts and subsequent chemical modification of the as-fabricated CNT(Fe)/HA by chitosan (CS) and folic acid (FA) for a controllable release of an anticancer drug doxorubicin (DOX).
30678906	1	35	theme	anticancer	473:482	arg1	doxorubicin					489:499	an anticancer drug doxorubicin	470:499	an anticancer drug doxorubicin (DOX)	470:505	A novel magnetic targeted drug delivery carrier based on a carbon nanotube (Fe)/hydroxyapatite (CNT(Fe)/HA) composite was successfully fabricated by an in situ synthesis of CNTs in HA nanopowder using Fe catalysts and subsequent chemical modification of the as-fabricated CNT(Fe)/HA by chitosan (CS) and folic acid (FA) for a controllable release of an anticancer drug doxorubicin (DOX).
30678906	0	36	theme	carbon	30:35	arg1	nanotube					37:44	carbon nanotube	30:44	carbon nanotube (Fe)	30:49	Preparation and properties of carbon nanotube (Fe)/hydroxyapatite composite as magnetic targeted drug delivery carrier.
30678906	0	36	theme	carbon	30:35	arg1	Fe					47:48	Fe	47:48	Fe	47:48	Preparation and properties of carbon nanotube (Fe)/hydroxyapatite composite as magnetic targeted drug delivery carrier.
30678906	4	37	with	coating	848:854	arg1	FA					868:869	FA	868:869	FA	868:869	Upon the functional modification through the surface coating with CS and FA, the functionalized CNT(Fe)/HA became capable of loading DOX through both π-π stacking and electrostatic adsorption of FA.
30678906	4	37	with	coating	848:854	arg1	CS					861:862	CS	861:862	CS	861:862	Upon the functional modification through the surface coating with CS and FA, the functionalized CNT(Fe)/HA became capable of loading DOX through both π-π stacking and electrostatic adsorption of FA.
30678906	1	38	from	synthesis	280:288	arg1	nanopowder					304:313	HA nanopowder	301:313	HA nanopowder using Fe catalysts and subsequent chemical modification of the as-fabricated CNT(Fe)/HA by chitosan (CS) and folic acid (FA) for a controllable release of an anticancer drug doxorubicin (DOX)	301:505	A novel magnetic targeted drug delivery carrier based on a carbon nanotube (Fe)/hydroxyapatite (CNT(Fe)/HA) composite was successfully fabricated by an in situ synthesis of CNTs in HA nanopowder using Fe catalysts and subsequent chemical modification of the as-fabricated CNT(Fe)/HA by chitosan (CS) and folic acid (FA) for a controllable release of an anticancer drug doxorubicin (DOX).
30678906	8	39	from	0.88 emu g-1	1466:1477	arg1	addition					1333:1340	addition	1333:1340	addition	1333:1340	In addition, the saturation magnetization, coercive force, and remanence to saturation magnetization ratio of DOX-FA-CS-CNT(Fe)/HA were 0.88 emu g-1, 668.96 Oe, and 0.44, respectively, indicating its potential for drug transport under strong external magnetic fields, which could enable magnetic targeted delivery.
30678906	5	40	theme	DOX	1051:1053	arg1	rate					1043:1046	the average drug-loading rate	1018:1046	the average drug-loading rate of DOX	1018:1053	The results showed that the average drug-loading rate of DOX was 130 wt%.
30678906	5	40	theme	DOX	1051:1053	arg1	%					1065:1065	130 wt%	1059:1065	130 wt%	1059:1065	The results showed that the average drug-loading rate of DOX was 130 wt%.
30678906	4	41	theme	loading	920:926	arg1	DOX					928:930	loading DOX	920:930	loading DOX	920:930	Upon the functional modification through the surface coating with CS and FA, the functionalized CNT(Fe)/HA became capable of loading DOX through both π-π stacking and electrostatic adsorption of FA.
30678906	6	42	theme	52 wt	1246:1250	arg1	%					1251:1251	52 wt%	1246:1251	52 wt%	1246:1251	Furthermore, the pH response of FA-CS-CNT(Fe)/HA enabled the release of a large amount of DOX in phosphate-buffered saline (PBS) at pH = 5.5 with an average drug release rate of 52 wt% after 72 h.
30678906	8	43	theme	magnetic	1581:1588	arg1	fields					1590:1595	strong external magnetic fields	1565:1595	strong external magnetic fields	1565:1595	In addition, the saturation magnetization, coercive force, and remanence to saturation magnetization ratio of DOX-FA-CS-CNT(Fe)/HA were 0.88 emu g-1, 668.96 Oe, and 0.44, respectively, indicating its potential for drug transport under strong external magnetic fields, which could enable magnetic targeted delivery.
30678906	4	44	theme	Fe	895:896	arg1	/HA					898:900	the functionalized CNT(Fe)/HA	872:900	the functionalized CNT(Fe)/HA	872:900	Upon the functional modification through the surface coating with CS and FA, the functionalized CNT(Fe)/HA became capable of loading DOX through both π-π stacking and electrostatic adsorption of FA.
30678906	6	45	theme	FA-CS-CNT	1100:1108	arg1	/HA					1113:1115	FA-CS-CNT(Fe)/HA	1100:1115	FA-CS-CNT(Fe)/HA	1100:1115	Furthermore, the pH response of FA-CS-CNT(Fe)/HA enabled the release of a large amount of DOX in phosphate-buffered saline (PBS) at pH = 5.5 with an average drug release rate of 52 wt% after 72 h.
30678906	5	46	theme	average	1022:1028	arg1	rate					1043:1046	the average drug-loading rate	1018:1046	the average drug-loading rate of DOX	1018:1053	The results showed that the average drug-loading rate of DOX was 130 wt%.
30678906	5	46	theme	average	1022:1028	arg1	%					1065:1065	130 wt%	1059:1065	130 wt%	1059:1065	The results showed that the average drug-loading rate of DOX was 130 wt%.
30678906	8	47	theme	saturation	1347:1356	arg1	magnetization					1358:1370	the saturation magnetization	1343:1370	the saturation magnetization	1343:1370	In addition, the saturation magnetization, coercive force, and remanence to saturation magnetization ratio of DOX-FA-CS-CNT(Fe)/HA were 0.88 emu g-1, 668.96 Oe, and 0.44, respectively, indicating its potential for drug transport under strong external magnetic fields, which could enable magnetic targeted delivery.
30678906	8	47	theme	saturation	1347:1356	arg1	0.88 emu g-1					1466:1477	0.88 emu g-1	1466:1477	0.88 emu g-1	1466:1477	In addition, the saturation magnetization, coercive force, and remanence to saturation magnetization ratio of DOX-FA-CS-CNT(Fe)/HA were 0.88 emu g-1, 668.96 Oe, and 0.44, respectively, indicating its potential for drug transport under strong external magnetic fields, which could enable magnetic targeted delivery.
30678906	1	48	theme	CNT	392:394	arg1	/HA					399:401	the as-fabricated CNT(Fe)/HA	374:401	the as-fabricated CNT(Fe)/HA	374:401	A novel magnetic targeted drug delivery carrier based on a carbon nanotube (Fe)/hydroxyapatite (CNT(Fe)/HA) composite was successfully fabricated by an in situ synthesis of CNTs in HA nanopowder using Fe catalysts and subsequent chemical modification of the as-fabricated CNT(Fe)/HA by chitosan (CS) and folic acid (FA) for a controllable release of an anticancer drug doxorubicin (DOX).
30678906	6	49	theme	drug	1225:1228	arg1	rate					1238:1241	an average drug release rate	1214:1241	an average drug release rate of 52 wt% after 72 h	1214:1262	Furthermore, the pH response of FA-CS-CNT(Fe)/HA enabled the release of a large amount of DOX in phosphate-buffered saline (PBS) at pH = 5.5 with an average drug release rate of 52 wt% after 72 h.
30678906	1	50	theme	Fe	396:397	arg1	/HA					399:401	the as-fabricated CNT(Fe)/HA	374:401	the as-fabricated CNT(Fe)/HA	374:401	A novel magnetic targeted drug delivery carrier based on a carbon nanotube (Fe)/hydroxyapatite (CNT(Fe)/HA) composite was successfully fabricated by an in situ synthesis of CNTs in HA nanopowder using Fe catalysts and subsequent chemical modification of the as-fabricated CNT(Fe)/HA by chitosan (CS) and folic acid (FA) for a controllable release of an anticancer drug doxorubicin (DOX).
30678906	8	51	theme	magnetic	1617:1624	arg1	delivery					1635:1642	magnetic targeted delivery	1617:1642	magnetic targeted delivery	1617:1642	In addition, the saturation magnetization, coercive force, and remanence to saturation magnetization ratio of DOX-FA-CS-CNT(Fe)/HA were 0.88 emu g-1, 668.96 Oe, and 0.44, respectively, indicating its potential for drug transport under strong external magnetic fields, which could enable magnetic targeted delivery.
30678906	1	52	theme	/HA	399:401	arg1	modification					358:369	subsequent chemical modification	338:369	subsequent chemical modification of the as-fabricated CNT(Fe)/HA by chitosan (CS) and folic acid (FA) for a controllable release of an anticancer drug doxorubicin (DOX)	338:505	A novel magnetic targeted drug delivery carrier based on a carbon nanotube (Fe)/hydroxyapatite (CNT(Fe)/HA) composite was successfully fabricated by an in situ synthesis of CNTs in HA nanopowder using Fe catalysts and subsequent chemical modification of the as-fabricated CNT(Fe)/HA by chitosan (CS) and folic acid (FA) for a controllable release of an anticancer drug doxorubicin (DOX).
30678906	1	52	theme	/HA	399:401	arg1	catalysts					324:332	Fe catalysts	321:332	Fe catalysts	321:332	A novel magnetic targeted drug delivery carrier based on a carbon nanotube (Fe)/hydroxyapatite (CNT(Fe)/HA) composite was successfully fabricated by an in situ synthesis of CNTs in HA nanopowder using Fe catalysts and subsequent chemical modification of the as-fabricated CNT(Fe)/HA by chitosan (CS) and folic acid (FA) for a controllable release of an anticancer drug doxorubicin (DOX).
30678906	3	53	theme	acid	623:626	arg1	treatment					628:636	the acid treatment	619:636	the acid treatment	619:636	After the acid treatment, the CNTs were shorter and homogeneously dispersed, the tips of the CNTs were opened, and oxygen-containing functional groups were introduced onto the CNTs.
30678906	1	54	theme	carbon	179:184	arg1	composite					228:236	a carbon nanotube (Fe)/hydroxyapatite (CNT(Fe)/HA) composite	177:236	a carbon nanotube (Fe)/hydroxyapatite (CNT(Fe)/HA) composite	177:236	A novel magnetic targeted drug delivery carrier based on a carbon nanotube (Fe)/hydroxyapatite (CNT(Fe)/HA) composite was successfully fabricated by an in situ synthesis of CNTs in HA nanopowder using Fe catalysts and subsequent chemical modification of the as-fabricated CNT(Fe)/HA by chitosan (CS) and folic acid (FA) for a controllable release of an anticancer drug doxorubicin (DOX).
30678906	4	55	theme	π-π	945:947	arg1	stacking					949:956	π-π stacking	945:956	π-π stacking	945:956	Upon the functional modification through the surface coating with CS and FA, the functionalized CNT(Fe)/HA became capable of loading DOX through both π-π stacking and electrostatic adsorption of FA.
30678906	8	56	dep	ratio	1431:1435	arg1	to					1403:1404	to	1403:1404	to	1403:1404	In addition, the saturation magnetization, coercive force, and remanence to saturation magnetization ratio of DOX-FA-CS-CNT(Fe)/HA were 0.88 emu g-1, 668.96 Oe, and 0.44, respectively, indicating its potential for drug transport under strong external magnetic fields, which could enable magnetic targeted delivery.
30678906	6	57	theme	/HA	1113:1115	arg1	response					1088:1095	the pH response	1081:1095	the pH response of FA-CS-CNT(Fe)/HA	1081:1115	Furthermore, the pH response of FA-CS-CNT(Fe)/HA enabled the release of a large amount of DOX in phosphate-buffered saline (PBS) at pH = 5.5 with an average drug release rate of 52 wt% after 72 h.
30678906	1	58	theme	subsequent	338:347	arg1	modification					358:369	subsequent chemical modification	338:369	subsequent chemical modification of the as-fabricated CNT(Fe)/HA by chitosan (CS) and folic acid (FA) for a controllable release of an anticancer drug doxorubicin (DOX)	338:505	A novel magnetic targeted drug delivery carrier based on a carbon nanotube (Fe)/hydroxyapatite (CNT(Fe)/HA) composite was successfully fabricated by an in situ synthesis of CNTs in HA nanopowder using Fe catalysts and subsequent chemical modification of the as-fabricated CNT(Fe)/HA by chitosan (CS) and folic acid (FA) for a controllable release of an anticancer drug doxorubicin (DOX).
30678906	2	59	theme	composite	563:571	arg1	structure					573:581	a composite structure	561:581	a composite structure	561:581	The synthesized CNTs, Fe, and HA self-assembled into a composite structure in situ during the synthesis.
30678906	1	60	theme	drug	484:487	arg1	DOX					502:504	DOX	502:504	DOX	502:504	A novel magnetic targeted drug delivery carrier based on a carbon nanotube (Fe)/hydroxyapatite (CNT(Fe)/HA) composite was successfully fabricated by an in situ synthesis of CNTs in HA nanopowder using Fe catalysts and subsequent chemical modification of the as-fabricated CNT(Fe)/HA by chitosan (CS) and folic acid (FA) for a controllable release of an anticancer drug doxorubicin (DOX).
30678906	1	60	theme	drug	484:487	arg1	doxorubicin					489:499	an anticancer drug doxorubicin	470:499	an anticancer drug doxorubicin (DOX)	470:505	A novel magnetic targeted drug delivery carrier based on a carbon nanotube (Fe)/hydroxyapatite (CNT(Fe)/HA) composite was successfully fabricated by an in situ synthesis of CNTs in HA nanopowder using Fe catalysts and subsequent chemical modification of the as-fabricated CNT(Fe)/HA by chitosan (CS) and folic acid (FA) for a controllable release of an anticancer drug doxorubicin (DOX).
30678906	6	61	theme	Fe	1110:1111	arg1	/HA					1113:1115	FA-CS-CNT(Fe)/HA	1100:1115	FA-CS-CNT(Fe)/HA	1100:1115	Furthermore, the pH response of FA-CS-CNT(Fe)/HA enabled the release of a large amount of DOX in phosphate-buffered saline (PBS) at pH = 5.5 with an average drug release rate of 52 wt% after 72 h.
30678906	4	62	theme	FA	990:991	arg1	stacking					949:956	π-π stacking	945:956	π-π stacking	945:956	Upon the functional modification through the surface coating with CS and FA, the functionalized CNT(Fe)/HA became capable of loading DOX through both π-π stacking and electrostatic adsorption of FA.
30678906	4	62	theme	FA	990:991	arg1	adsorption					976:985	electrostatic adsorption	962:985	electrostatic adsorption	962:985	Upon the functional modification through the surface coating with CS and FA, the functionalized CNT(Fe)/HA became capable of loading DOX through both π-π stacking and electrostatic adsorption of FA.
30678906	1	63	theme	magnetic	128:135	arg1	carrier					160:166	A novel magnetic targeted drug delivery carrier	120:166	A novel magnetic targeted drug delivery carrier based on a carbon nanotube (Fe)/hydroxyapatite (CNT(Fe)/HA) composite	120:236	A novel magnetic targeted drug delivery carrier based on a carbon nanotube (Fe)/hydroxyapatite (CNT(Fe)/HA) composite was successfully fabricated by an in situ synthesis of CNTs in HA nanopowder using Fe catalysts and subsequent chemical modification of the as-fabricated CNT(Fe)/HA by chitosan (CS) and folic acid (FA) for a controllable release of an anticancer drug doxorubicin (DOX).
30678906	6	64	theme	amount	1148:1153	arg1	release					1129:1135	the release	1125:1135	the release of a large amount of DOX in phosphate-buffered saline (PBS)	1125:1195	Furthermore, the pH response of FA-CS-CNT(Fe)/HA enabled the release of a large amount of DOX in phosphate-buffered saline (PBS) at pH = 5.5 with an average drug release rate of 52 wt% after 72 h.
30678906	1	65	theme	drug	146:149	arg1	carrier					160:166	A novel magnetic targeted drug delivery carrier	120:166	A novel magnetic targeted drug delivery carrier based on a carbon nanotube (Fe)/hydroxyapatite (CNT(Fe)/HA) composite	120:236	A novel magnetic targeted drug delivery carrier based on a carbon nanotube (Fe)/hydroxyapatite (CNT(Fe)/HA) composite was successfully fabricated by an in situ synthesis of CNTs in HA nanopowder using Fe catalysts and subsequent chemical modification of the as-fabricated CNT(Fe)/HA by chitosan (CS) and folic acid (FA) for a controllable release of an anticancer drug doxorubicin (DOX).
30678906	3	66	theme	oxygen-containing	728:744	arg1	groups					757:762	oxygen-containing functional groups	728:762	oxygen-containing functional groups	728:762	After the acid treatment, the CNTs were shorter and homogeneously dispersed, the tips of the CNTs were opened, and oxygen-containing functional groups were introduced onto the CNTs.
30678906	4	67	theme	surface	840:846	arg1	coating					848:854	the surface coating	836:854	the surface coating with CS and FA	836:869	Upon the functional modification through the surface coating with CS and FA, the functionalized CNT(Fe)/HA became capable of loading DOX through both π-π stacking and electrostatic adsorption of FA.
30678906	0	68	theme	targeted	88:95	arg1	delivery					102:109	magnetic targeted drug delivery	79:109	magnetic targeted drug delivery carrier	79:117	Preparation and properties of carbon nanotube (Fe)/hydroxyapatite composite as magnetic targeted drug delivery carrier.
30678906	1	69	theme	CNTs	293:296	arg1	synthesis					280:288	an in situ synthesis	269:288	an in situ synthesis of CNTs in HA nanopowder using Fe catalysts and subsequent chemical modification of the as-fabricated CNT(Fe)/HA by chitosan (CS) and folic acid (FA) for a controllable release of an anticancer drug doxorubicin (DOX)	269:505	A novel magnetic targeted drug delivery carrier based on a carbon nanotube (Fe)/hydroxyapatite (CNT(Fe)/HA) composite was successfully fabricated by an in situ synthesis of CNTs in HA nanopowder using Fe catalysts and subsequent chemical modification of the as-fabricated CNT(Fe)/HA by chitosan (CS) and folic acid (FA) for a controllable release of an anticancer drug doxorubicin (DOX).
30678906	0	70	theme	delivery	102:109	arg1	carrier					111:117	magnetic targeted drug delivery carrier	79:117	magnetic targeted drug delivery carrier	79:117	Preparation and properties of carbon nanotube (Fe)/hydroxyapatite composite as magnetic targeted drug delivery carrier.
30678906	1	71	theme	HA	301:302	arg1	nanopowder					304:313	HA nanopowder	301:313	HA nanopowder using Fe catalysts and subsequent chemical modification of the as-fabricated CNT(Fe)/HA by chitosan (CS) and folic acid (FA) for a controllable release of an anticancer drug doxorubicin (DOX)	301:505	A novel magnetic targeted drug delivery carrier based on a carbon nanotube (Fe)/hydroxyapatite (CNT(Fe)/HA) composite was successfully fabricated by an in situ synthesis of CNTs in HA nanopowder using Fe catalysts and subsequent chemical modification of the as-fabricated CNT(Fe)/HA by chitosan (CS) and folic acid (FA) for a controllable release of an anticancer drug doxorubicin (DOX).
30678906	8	72	theme	remanence	1393:1401	arg1	ratio					1431:1435	remanence to saturation magnetization ratio	1393:1435	remanence to saturation magnetization ratio of DOX-FA-CS-CNT(Fe)/HA	1393:1459	In addition, the saturation magnetization, coercive force, and remanence to saturation magnetization ratio of DOX-FA-CS-CNT(Fe)/HA were 0.88 emu g-1, 668.96 Oe, and 0.44, respectively, indicating its potential for drug transport under strong external magnetic fields, which could enable magnetic targeted delivery.
30678906	4	73	theme	functional	804:813	arg1	modification					815:826	the functional modification	800:826	the functional modification through the surface coating with CS and FA	800:869	Upon the functional modification through the surface coating with CS and FA, the functionalized CNT(Fe)/HA became capable of loading DOX through both π-π stacking and electrostatic adsorption of FA.
30678906	6	74	theme	large	1142:1146	arg1	amount					1148:1153	a large amount	1140:1153	a large amount of DOX	1140:1160	Furthermore, the pH response of FA-CS-CNT(Fe)/HA enabled the release of a large amount of DOX in phosphate-buffered saline (PBS) at pH = 5.5 with an average drug release rate of 52 wt% after 72 h.
30678906	6	74	theme	large	1142:1146	arg1	DOX					1158:1160	DOX	1158:1160	DOX	1158:1160	Furthermore, the pH response of FA-CS-CNT(Fe)/HA enabled the release of a large amount of DOX in phosphate-buffered saline (PBS) at pH = 5.5 with an average drug release rate of 52 wt% after 72 h.
30678906	6	75	theme	average	1217:1223	arg1	rate					1238:1241	an average drug release rate	1214:1241	an average drug release rate of 52 wt% after 72 h	1214:1262	Furthermore, the pH response of FA-CS-CNT(Fe)/HA enabled the release of a large amount of DOX in phosphate-buffered saline (PBS) at pH = 5.5 with an average drug release rate of 52 wt% after 72 h.
30678906	8	76	theme	drug	1544:1547	arg1	transport					1549:1557	drug transport	1544:1557	drug transport	1544:1557	In addition, the saturation magnetization, coercive force, and remanence to saturation magnetization ratio of DOX-FA-CS-CNT(Fe)/HA were 0.88 emu g-1, 668.96 Oe, and 0.44, respectively, indicating its potential for drug transport under strong external magnetic fields, which could enable magnetic targeted delivery.
30678906	4	77	theme	functionalized	876:889	arg1	/HA					898:900	the functionalized CNT(Fe)/HA	872:900	the functionalized CNT(Fe)/HA	872:900	Upon the functional modification through the surface coating with CS and FA, the functionalized CNT(Fe)/HA became capable of loading DOX through both π-π stacking and electrostatic adsorption of FA.
30678906	0	78	theme	nanotube	37:44	arg1	properties					16:25	properties	16:25	properties of carbon nanotube (Fe)	16:49	Preparation and properties of carbon nanotube (Fe)/hydroxyapatite composite as magnetic targeted drug delivery carrier.
30678906	0	78	theme	nanotube	37:44	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and properties of carbon nanotube (Fe)/hydroxyapatite composite as magnetic targeted drug delivery carrier.
30678906	6	79	from	release	1129:1135	arg1	PBS					1192:1194	PBS	1192:1194	PBS	1192:1194	Furthermore, the pH response of FA-CS-CNT(Fe)/HA enabled the release of a large amount of DOX in phosphate-buffered saline (PBS) at pH = 5.5 with an average drug release rate of 52 wt% after 72 h.
30678906	6	79	from	release	1129:1135	arg1	saline					1184:1189	phosphate-buffered saline	1165:1189	phosphate-buffered saline (PBS)	1165:1195	Furthermore, the pH response of FA-CS-CNT(Fe)/HA enabled the release of a large amount of DOX in phosphate-buffered saline (PBS) at pH = 5.5 with an average drug release rate of 52 wt% after 72 h.
30678906	7	80	from	pH = 7.4	1305:1312	arg1	%					1327:1327	only 8 wt%	1318:1327	only 8 wt%	1318:1327	In contrast, the drug release in PBS at pH = 7.4 was only 8 wt%.
30678906	7	80	from	pH = 7.4	1305:1312	arg1	release					1287:1293	the drug release	1278:1293	the drug release in PBS at pH = 7.4	1278:1312	In contrast, the drug release in PBS at pH = 7.4 was only 8 wt%.
30678906	7	81	theme	drug	1282:1285	arg1	%					1327:1327	only 8 wt%	1318:1327	only 8 wt%	1318:1327	In contrast, the drug release in PBS at pH = 7.4 was only 8 wt%.
30678906	7	81	theme	drug	1282:1285	arg1	release					1287:1293	the drug release	1278:1293	the drug release in PBS at pH = 7.4	1278:1312	In contrast, the drug release in PBS at pH = 7.4 was only 8 wt%.
30678906	1	82	theme	in	272:273	arg1	synthesis					280:288	an in situ synthesis	269:288	an in situ synthesis of CNTs in HA nanopowder using Fe catalysts and subsequent chemical modification of the as-fabricated CNT(Fe)/HA by chitosan (CS) and folic acid (FA) for a controllable release of an anticancer drug doxorubicin (DOX)	269:505	A novel magnetic targeted drug delivery carrier based on a carbon nanotube (Fe)/hydroxyapatite (CNT(Fe)/HA) composite was successfully fabricated by an in situ synthesis of CNTs in HA nanopowder using Fe catalysts and subsequent chemical modification of the as-fabricated CNT(Fe)/HA by chitosan (CS) and folic acid (FA) for a controllable release of an anticancer drug doxorubicin (DOX).
30678906	7	83	from	%	1327:1327	arg1	contrast					1268:1275	contrast	1268:1275	contrast	1268:1275	In contrast, the drug release in PBS at pH = 7.4 was only 8 wt%.
30678906	1	84	theme	folic	424:428	arg1	acid					430:433	folic acid	424:433	folic acid	424:433	A novel magnetic targeted drug delivery carrier based on a carbon nanotube (Fe)/hydroxyapatite (CNT(Fe)/HA) composite was successfully fabricated by an in situ synthesis of CNTs in HA nanopowder using Fe catalysts and subsequent chemical modification of the as-fabricated CNT(Fe)/HA by chitosan (CS) and folic acid (FA) for a controllable release of an anticancer drug doxorubicin (DOX).
30678906	6	85	theme	%	1251:1251	arg1	rate					1238:1241	an average drug release rate	1214:1241	an average drug release rate of 52 wt% after 72 h	1214:1262	Furthermore, the pH response of FA-CS-CNT(Fe)/HA enabled the release of a large amount of DOX in phosphate-buffered saline (PBS) at pH = 5.5 with an average drug release rate of 52 wt% after 72 h.
30678906	1	86	theme	/hydroxyapatite	199:213	arg1	composite					228:236	a carbon nanotube (Fe)/hydroxyapatite (CNT(Fe)/HA) composite	177:236	a carbon nanotube (Fe)/hydroxyapatite (CNT(Fe)/HA) composite	177:236	A novel magnetic targeted drug delivery carrier based on a carbon nanotube (Fe)/hydroxyapatite (CNT(Fe)/HA) composite was successfully fabricated by an in situ synthesis of CNTs in HA nanopowder using Fe catalysts and subsequent chemical modification of the as-fabricated CNT(Fe)/HA by chitosan (CS) and folic acid (FA) for a controllable release of an anticancer drug doxorubicin (DOX).
30678906	8	87	theme	saturation	1406:1415	arg1	ratio					1431:1435	remanence to saturation magnetization ratio	1393:1435	remanence to saturation magnetization ratio of DOX-FA-CS-CNT(Fe)/HA	1393:1459	In addition, the saturation magnetization, coercive force, and remanence to saturation magnetization ratio of DOX-FA-CS-CNT(Fe)/HA were 0.88 emu g-1, 668.96 Oe, and 0.44, respectively, indicating its potential for drug transport under strong external magnetic fields, which could enable magnetic targeted delivery.
30678906	1	88	theme	CNT	216:218	arg1	composite					228:236	a carbon nanotube (Fe)/hydroxyapatite (CNT(Fe)/HA) composite	177:236	a carbon nanotube (Fe)/hydroxyapatite (CNT(Fe)/HA) composite	177:236	A novel magnetic targeted drug delivery carrier based on a carbon nanotube (Fe)/hydroxyapatite (CNT(Fe)/HA) composite was successfully fabricated by an in situ synthesis of CNTs in HA nanopowder using Fe catalysts and subsequent chemical modification of the as-fabricated CNT(Fe)/HA by chitosan (CS) and folic acid (FA) for a controllable release of an anticancer drug doxorubicin (DOX).
29751604	4	0	theme	rat	837:839	arg1	specimens					850:858	rat prostate specimens	837:858	rat prostate specimens	837:858	The aim of the present work was to evaluate the protective effects of Graminex pollen (GraminexTM, Deshler, OH, USA), a commercially available product based on standardized pollen extracts, in rat prostate specimens, ex vivo.
29751604	2	1	theme	herbal	326:331	arg1	extracts					333:340	herbal extracts	326:340	herbal extracts with antioxidant and anti-inflammatory effects for blunting the burden of inflammation and oxidative stress	326:448	The efficacy of herbal extracts with antioxidant and anti-inflammatory effects for blunting the burden of inflammation and oxidative stress, with possible improvements in clinical symptoms, is under investigation.
29751604	5	2	from	mechanism	911:919	arg1	reduction					990:998	the reduction	986:998	the reduction of prostaglandin E₂ (PGE₂), nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB), and malondialdehyde (MDA)	986:1123	In this context, we studied the putative mechanism of action of pollen on multiple inflammatory pathways, including the reduction of prostaglandin E₂ (PGE₂), nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB), and malondialdehyde (MDA), whose activities were significantly increased by inflammatory stimuli.
29751604	5	2	from	mechanism	911:919	arg1	pathways					966:973	multiple inflammatory pathways	944:973	multiple inflammatory pathways	944:973	In this context, we studied the putative mechanism of action of pollen on multiple inflammatory pathways, including the reduction of prostaglandin E₂ (PGE₂), nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB), and malondialdehyde (MDA), whose activities were significantly increased by inflammatory stimuli.
29751604	4	3	theme	pollen	817:822	arg1	extracts					824:831	standardized pollen extracts	804:831	standardized pollen extracts	804:831	The aim of the present work was to evaluate the protective effects of Graminex pollen (GraminexTM, Deshler, OH, USA), a commercially available product based on standardized pollen extracts, in rat prostate specimens, ex vivo.
29751604	4	4	theme	present	659:665	arg1	work					667:670	the present work	655:670	the present work	655:670	The aim of the present work was to evaluate the protective effects of Graminex pollen (GraminexTM, Deshler, OH, USA), a commercially available product based on standardized pollen extracts, in rat prostate specimens, ex vivo.
29751604	8	5	theme	components	1775:1784	arg1	management					1744:1753	the management	1740:1753	the management of the inflammatory components in the prostate	1740:1800	According to our experimental evidence, Graminex pollen appears to be a promising natural product for the management of the inflammatory components in the prostate.
29751604	3	6	theme	promising	573:581	arg1	agents					583:588	promising agents	573:588	promising agents	573:588	Pollen extracts have been previously reported as promising agents in managing clinical symptoms related to prostatitis.
29751604	3	6	theme	promising	573:581	arg1	extracts					531:538	Pollen extracts	524:538	Pollen extracts	524:538	Pollen extracts have been previously reported as promising agents in managing clinical symptoms related to prostatitis.
29751604	6	7	theme	Graminex	1286:1293	arg1	pollen					1295:1300	Graminex pollen	1286:1300	Graminex pollen	1286:1300	We characterized by means of chromatographic and colorimetric studies the composition of Graminex pollen to better correlate the activity of pollen on immortalized prostate cells (PC3), and in rat prostate specimens challenged with Escherichia coli lipopolysaccharide (LPS).
29751604	5	8	from	pathways	966:973	arg1	mechanism					911:919	the putative mechanism	898:919	the putative mechanism of action of pollen on multiple inflammatory pathways, including the reduction of prostaglandin E₂ (PGE₂), nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB), and malondialdehyde (MDA), whose activities were significantly increased by inflammatory stimuli	898:1194	In this context, we studied the putative mechanism of action of pollen on multiple inflammatory pathways, including the reduction of prostaglandin E₂ (PGE₂), nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB), and malondialdehyde (MDA), whose activities were significantly increased by inflammatory stimuli.
29751604	6	9	theme	rat	1390:1392	arg1	specimens					1403:1411	rat prostate specimens	1390:1411	rat prostate specimens challenged with Escherichia coli lipopolysaccharide (LPS)	1390:1469	We characterized by means of chromatographic and colorimetric studies the composition of Graminex pollen to better correlate the activity of pollen on immortalized prostate cells (PC3), and in rat prostate specimens challenged with Escherichia coli lipopolysaccharide (LPS).
29751604	3	10	theme	clinical	602:609	arg1	symptoms					611:618	clinical symptoms	602:618	clinical symptoms related to prostatitis	602:641	Pollen extracts have been previously reported as promising agents in managing clinical symptoms related to prostatitis.
29751604	1	11	theme	prostate	194:201	arg1	inflammation					203:214	prostate inflammation	194:214	prostate inflammation	194:214	Prostatitis, a general term describing prostate inflammation, is a common disease that could be sustained by bacterial or non-bacterial infectious agents.
29751604	5	12	theme	prostaglandin	1003:1015	arg1	PGE₂					1021:1024	PGE₂	1021:1024	PGE₂	1021:1024	In this context, we studied the putative mechanism of action of pollen on multiple inflammatory pathways, including the reduction of prostaglandin E₂ (PGE₂), nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB), and malondialdehyde (MDA), whose activities were significantly increased by inflammatory stimuli.
29751604	5	12	theme	prostaglandin	1003:1015	arg1	E₂					1017:1018	prostaglandin E₂	1003:1018	prostaglandin E₂ (PGE₂)	1003:1025	In this context, we studied the putative mechanism of action of pollen on multiple inflammatory pathways, including the reduction of prostaglandin E₂ (PGE₂), nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB), and malondialdehyde (MDA), whose activities were significantly increased by inflammatory stimuli.
29751604	8	13	theme	Graminex	1678:1685	arg1	product					1728:1734	a promising natural product	1708:1734	a promising natural product for the management of the inflammatory components in the prostate	1708:1800	According to our experimental evidence, Graminex pollen appears to be a promising natural product for the management of the inflammatory components in the prostate.
29751604	8	13	theme	Graminex	1678:1685	arg1	pollen					1687:1692	Graminex pollen	1678:1692	Graminex pollen	1678:1692	According to our experimental evidence, Graminex pollen appears to be a promising natural product for the management of the inflammatory components in the prostate.
29751604	7	14	theme	prostate	1618:1625	arg1	specimens					1627:1635	rat prostate specimens	1614:1635	rat prostate specimens	1614:1635	We found that Graminex pollen was able to reduce radical oxygen species (ROS) production by PC3 cells and MDA, NFκB mRNA, and PGE₂ levels, in rat prostate specimens.
29751604	7	15	theme	oxygen	1529:1534	arg1	ROS					1545:1547	ROS	1545:1547	ROS	1545:1547	We found that Graminex pollen was able to reduce radical oxygen species (ROS) production by PC3 cells and MDA, NFκB mRNA, and PGE₂ levels, in rat prostate specimens.
29751604	7	15	theme	oxygen	1529:1534	arg1	species					1536:1542	radical oxygen species	1521:1542	radical oxygen species (ROS) production by PC3 cells and MDA	1521:1580	We found that Graminex pollen was able to reduce radical oxygen species (ROS) production by PC3 cells and MDA, NFκB mRNA, and PGE₂ levels, in rat prostate specimens.
29751604	6	16	theme	pollen	1338:1343	arg1	activity					1326:1333	the activity	1322:1333	the activity of pollen	1322:1343	We characterized by means of chromatographic and colorimetric studies the composition of Graminex pollen to better correlate the activity of pollen on immortalized prostate cells (PC3), and in rat prostate specimens challenged with Escherichia coli lipopolysaccharide (LPS).
29751604	5	17	theme	activated	1073:1081	arg1	cells					1085:1089	activated B cells	1073:1089	activated B cells	1073:1089	In this context, we studied the putative mechanism of action of pollen on multiple inflammatory pathways, including the reduction of prostaglandin E₂ (PGE₂), nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB), and malondialdehyde (MDA), whose activities were significantly increased by inflammatory stimuli.
29751604	3	18	theme	related	620:626	arg1	symptoms					611:618	clinical symptoms	602:618	clinical symptoms related to prostatitis	602:641	Pollen extracts have been previously reported as promising agents in managing clinical symptoms related to prostatitis.
29751604	2	19	theme	clinical	481:488	arg1	symptoms					490:497	clinical symptoms	481:497	clinical symptoms	481:497	The efficacy of herbal extracts with antioxidant and anti-inflammatory effects for blunting the burden of inflammation and oxidative stress, with possible improvements in clinical symptoms, is under investigation.
29751604	4	20	theme	protective	692:701	arg1	effects					703:709	the protective effects	688:709	the protective effects of Graminex pollen (GraminexTM, Deshler, OH, USA), a commercially available product based on standardized pollen extracts	688:831	The aim of the present work was to evaluate the protective effects of Graminex pollen (GraminexTM, Deshler, OH, USA), a commercially available product based on standardized pollen extracts, in rat prostate specimens, ex vivo.
29751604	5	21	theme	cells	1085:1089	arg1	malondialdehyde					1103:1117	malondialdehyde	1103:1117	malondialdehyde (MDA)	1103:1123	In this context, we studied the putative mechanism of action of pollen on multiple inflammatory pathways, including the reduction of prostaglandin E₂ (PGE₂), nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB), and malondialdehyde (MDA), whose activities were significantly increased by inflammatory stimuli.
29751604	5	21	theme	cells	1085:1089	arg1	E₂					1017:1018	prostaglandin E₂	1003:1018	prostaglandin E₂ (PGE₂)	1003:1025	In this context, we studied the putative mechanism of action of pollen on multiple inflammatory pathways, including the reduction of prostaglandin E₂ (PGE₂), nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB), and malondialdehyde (MDA), whose activities were significantly increased by inflammatory stimuli.
29751604	5	21	theme	cells	1085:1089	arg1	MDA					1120:1122	MDA	1120:1122	MDA	1120:1122	In this context, we studied the putative mechanism of action of pollen on multiple inflammatory pathways, including the reduction of prostaglandin E₂ (PGE₂), nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB), and malondialdehyde (MDA), whose activities were significantly increased by inflammatory stimuli.
29751604	5	21	theme	cells	1085:1089	arg1	kappa-light-chain-enhancer					1043:1068	nuclear factor kappa-light-chain-enhancer	1028:1068	nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB)	1028:1096	In this context, we studied the putative mechanism of action of pollen on multiple inflammatory pathways, including the reduction of prostaglandin E₂ (PGE₂), nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB), and malondialdehyde (MDA), whose activities were significantly increased by inflammatory stimuli.
29751604	5	21	theme	cells	1085:1089	arg1	NFκB					1092:1095	NFκB	1092:1095	NFκB	1092:1095	In this context, we studied the putative mechanism of action of pollen on multiple inflammatory pathways, including the reduction of prostaglandin E₂ (PGE₂), nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB), and malondialdehyde (MDA), whose activities were significantly increased by inflammatory stimuli.
29751604	5	21	theme	cells	1085:1089	arg1	PGE₂					1021:1024	PGE₂	1021:1024	PGE₂	1021:1024	In this context, we studied the putative mechanism of action of pollen on multiple inflammatory pathways, including the reduction of prostaglandin E₂ (PGE₂), nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB), and malondialdehyde (MDA), whose activities were significantly increased by inflammatory stimuli.
29751604	0	22	theme	Immortalized	83:94	arg1	PC3					112:114	PC3	112:114	PC3	112:114	Graminex Pollen: Phenolic Pattern, Colorimetric Analysis and Protective Effects in Immortalized Prostate Cells (PC3) and Rat Prostate Challenged with LPS.
29751604	0	22	theme	Immortalized	83:94	arg1	Cells					105:109	Immortalized Prostate Cells	83:109	Immortalized Prostate Cells (PC3)	83:115	Graminex Pollen: Phenolic Pattern, Colorimetric Analysis and Protective Effects in Immortalized Prostate Cells (PC3) and Rat Prostate Challenged with LPS.
29751604	5	23	theme	nuclear	1028:1034	arg1	kappa-light-chain-enhancer					1043:1068	nuclear factor kappa-light-chain-enhancer	1028:1068	nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB)	1028:1096	In this context, we studied the putative mechanism of action of pollen on multiple inflammatory pathways, including the reduction of prostaglandin E₂ (PGE₂), nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB), and malondialdehyde (MDA), whose activities were significantly increased by inflammatory stimuli.
29751604	5	23	theme	nuclear	1028:1034	arg1	NFκB					1092:1095	NFκB	1092:1095	NFκB	1092:1095	In this context, we studied the putative mechanism of action of pollen on multiple inflammatory pathways, including the reduction of prostaglandin E₂ (PGE₂), nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB), and malondialdehyde (MDA), whose activities were significantly increased by inflammatory stimuli.
29751604	8	24	theme	promising	1710:1718	arg1	product					1728:1734	a promising natural product	1708:1734	a promising natural product for the management of the inflammatory components in the prostate	1708:1800	According to our experimental evidence, Graminex pollen appears to be a promising natural product for the management of the inflammatory components in the prostate.
29751604	8	24	theme	promising	1710:1718	arg1	pollen					1687:1692	Graminex pollen	1678:1692	Graminex pollen	1678:1692	According to our experimental evidence, Graminex pollen appears to be a promising natural product for the management of the inflammatory components in the prostate.
29751604	5	25	theme	kappa-light-chain-enhancer	1043:1068	arg1	reduction					990:998	the reduction	986:998	the reduction of prostaglandin E₂ (PGE₂), nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB), and malondialdehyde (MDA)	986:1123	In this context, we studied the putative mechanism of action of pollen on multiple inflammatory pathways, including the reduction of prostaglandin E₂ (PGE₂), nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB), and malondialdehyde (MDA), whose activities were significantly increased by inflammatory stimuli.
29751604	2	26	theme	stress	443:448	arg1	burden					406:411	the burden	402:411	the burden of inflammation and oxidative stress	402:448	The efficacy of herbal extracts with antioxidant and anti-inflammatory effects for blunting the burden of inflammation and oxidative stress, with possible improvements in clinical symptoms, is under investigation.
29751604	0	27	from	Pattern	26:32	arg1	PC3					112:114	PC3	112:114	PC3	112:114	Graminex Pollen: Phenolic Pattern, Colorimetric Analysis and Protective Effects in Immortalized Prostate Cells (PC3) and Rat Prostate Challenged with LPS.
29751604	0	27	from	Pattern	26:32	arg1	Cells					105:109	Immortalized Prostate Cells	83:109	Immortalized Prostate Cells (PC3)	83:115	Graminex Pollen: Phenolic Pattern, Colorimetric Analysis and Protective Effects in Immortalized Prostate Cells (PC3) and Rat Prostate Challenged with LPS.
29751604	0	27	from	Pattern	26:32	arg1	Prostate					125:132	Rat Prostate	121:132	Rat Prostate	121:132	Graminex Pollen: Phenolic Pattern, Colorimetric Analysis and Protective Effects in Immortalized Prostate Cells (PC3) and Rat Prostate Challenged with LPS.
29751604	2	28	theme	anti-inflammatory	363:379	arg1	effects					381:387	antioxidant and anti-inflammatory effects	347:387	antioxidant and anti-inflammatory effects for blunting the burden of inflammation and oxidative stress	347:448	The efficacy of herbal extracts with antioxidant and anti-inflammatory effects for blunting the burden of inflammation and oxidative stress, with possible improvements in clinical symptoms, is under investigation.
29751604	0	29	theme	Graminex	0:7	arg1	Pollen					9:14	Graminex Pollen	0:14	Graminex Pollen: Phenolic Pattern, Colorimetric Analysis and Protective Effects in Immortalized Prostate Cells (PC3) and Rat Prostate Challenged with LPS.	0:153	Graminex Pollen: Phenolic Pattern, Colorimetric Analysis and Protective Effects in Immortalized Prostate Cells (PC3) and Rat Prostate Challenged with LPS.
29751604	5	30	theme	malondialdehyde	1103:1117	arg1	reduction					990:998	the reduction	986:998	the reduction of prostaglandin E₂ (PGE₂), nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB), and malondialdehyde (MDA)	986:1123	In this context, we studied the putative mechanism of action of pollen on multiple inflammatory pathways, including the reduction of prostaglandin E₂ (PGE₂), nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB), and malondialdehyde (MDA), whose activities were significantly increased by inflammatory stimuli.
29751604	4	31	dep	Graminex	714:721	arg1	OH					752:753	OH	752:753	OH	752:753	The aim of the present work was to evaluate the protective effects of Graminex pollen (GraminexTM, Deshler, OH, USA), a commercially available product based on standardized pollen extracts, in rat prostate specimens, ex vivo.
29751604	4	31	dep	Graminex	714:721	arg1	Deshler					743:749	Deshler	743:749	Deshler	743:749	The aim of the present work was to evaluate the protective effects of Graminex pollen (GraminexTM, Deshler, OH, USA), a commercially available product based on standardized pollen extracts, in rat prostate specimens, ex vivo.
29751604	4	31	dep	Graminex	714:721	arg1	USA					756:758	USA	756:758	USA	756:758	The aim of the present work was to evaluate the protective effects of Graminex pollen (GraminexTM, Deshler, OH, USA), a commercially available product based on standardized pollen extracts, in rat prostate specimens, ex vivo.
29751604	5	32	theme	putative	902:909	arg1	mechanism					911:919	the putative mechanism	898:919	the putative mechanism of action of pollen on multiple inflammatory pathways, including the reduction of prostaglandin E₂ (PGE₂), nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB), and malondialdehyde (MDA), whose activities were significantly increased by inflammatory stimuli	898:1194	In this context, we studied the putative mechanism of action of pollen on multiple inflammatory pathways, including the reduction of prostaglandin E₂ (PGE₂), nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB), and malondialdehyde (MDA), whose activities were significantly increased by inflammatory stimuli.
29751604	8	33	from	management	1744:1753	arg1	prostate					1793:1800	the prostate	1789:1800	the prostate	1789:1800	According to our experimental evidence, Graminex pollen appears to be a promising natural product for the management of the inflammatory components in the prostate.
29751604	7	34	from	able	1506:1509	arg1	specimens					1627:1635	rat prostate specimens	1614:1635	rat prostate specimens	1614:1635	We found that Graminex pollen was able to reduce radical oxygen species (ROS) production by PC3 cells and MDA, NFκB mRNA, and PGE₂ levels, in rat prostate specimens.
29751604	6	35	theme	immortalized	1348:1359	arg1	cells					1370:1374	immortalized prostate cells	1348:1374	immortalized prostate cells (PC3)	1348:1380	We characterized by means of chromatographic and colorimetric studies the composition of Graminex pollen to better correlate the activity of pollen on immortalized prostate cells (PC3), and in rat prostate specimens challenged with Escherichia coli lipopolysaccharide (LPS).
29751604	6	35	theme	immortalized	1348:1359	arg1	PC3					1377:1379	PC3	1377:1379	PC3	1377:1379	We characterized by means of chromatographic and colorimetric studies the composition of Graminex pollen to better correlate the activity of pollen on immortalized prostate cells (PC3), and in rat prostate specimens challenged with Escherichia coli lipopolysaccharide (LPS).
29751604	1	36	theme	infectious	291:300	arg1	agents					302:307	bacterial or non-bacterial infectious agents	264:307	bacterial or non-bacterial infectious agents	264:307	Prostatitis, a general term describing prostate inflammation, is a common disease that could be sustained by bacterial or non-bacterial infectious agents.
29751604	2	37	with	extracts	333:340	arg1	effects					381:387	antioxidant and anti-inflammatory effects	347:387	antioxidant and anti-inflammatory effects for blunting the burden of inflammation and oxidative stress	347:448	The efficacy of herbal extracts with antioxidant and anti-inflammatory effects for blunting the burden of inflammation and oxidative stress, with possible improvements in clinical symptoms, is under investigation.
29751604	8	38	from	components	1775:1784	arg1	prostate					1793:1800	the prostate	1789:1800	the prostate	1789:1800	According to our experimental evidence, Graminex pollen appears to be a promising natural product for the management of the inflammatory components in the prostate.
29751604	6	39	theme	Escherichia	1429:1439	arg1	LPS					1466:1468	LPS	1466:1468	LPS	1466:1468	We characterized by means of chromatographic and colorimetric studies the composition of Graminex pollen to better correlate the activity of pollen on immortalized prostate cells (PC3), and in rat prostate specimens challenged with Escherichia coli lipopolysaccharide (LPS).
29751604	6	39	theme	Escherichia	1429:1439	arg1	lipopolysaccharide					1446:1463	Escherichia coli lipopolysaccharide	1429:1463	Escherichia coli lipopolysaccharide (LPS)	1429:1469	We characterized by means of chromatographic and colorimetric studies the composition of Graminex pollen to better correlate the activity of pollen on immortalized prostate cells (PC3), and in rat prostate specimens challenged with Escherichia coli lipopolysaccharide (LPS).
29751604	2	40	theme	antioxidant	347:357	arg1	effects					381:387	antioxidant and anti-inflammatory effects	347:387	antioxidant and anti-inflammatory effects for blunting the burden of inflammation and oxidative stress	347:448	The efficacy of herbal extracts with antioxidant and anti-inflammatory effects for blunting the burden of inflammation and oxidative stress, with possible improvements in clinical symptoms, is under investigation.
29751604	5	41	theme	inflammatory	953:964	arg1	reduction					990:998	the reduction	986:998	the reduction of prostaglandin E₂ (PGE₂), nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB), and malondialdehyde (MDA)	986:1123	In this context, we studied the putative mechanism of action of pollen on multiple inflammatory pathways, including the reduction of prostaglandin E₂ (PGE₂), nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB), and malondialdehyde (MDA), whose activities were significantly increased by inflammatory stimuli.
29751604	5	41	theme	inflammatory	953:964	arg1	pathways					966:973	multiple inflammatory pathways	944:973	multiple inflammatory pathways	944:973	In this context, we studied the putative mechanism of action of pollen on multiple inflammatory pathways, including the reduction of prostaglandin E₂ (PGE₂), nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB), and malondialdehyde (MDA), whose activities were significantly increased by inflammatory stimuli.
29751604	6	42	theme	chromatographic	1226:1240	arg1	studies					1259:1265	chromatographic and colorimetric studies	1226:1265	chromatographic and colorimetric studies	1226:1265	We characterized by means of chromatographic and colorimetric studies the composition of Graminex pollen to better correlate the activity of pollen on immortalized prostate cells (PC3), and in rat prostate specimens challenged with Escherichia coli lipopolysaccharide (LPS).
29751604	2	43	theme	extracts	333:340	arg1	efficacy					314:321	The efficacy	310:321	The efficacy	310:321	The efficacy of herbal extracts with antioxidant and anti-inflammatory effects for blunting the burden of inflammation and oxidative stress, with possible improvements in clinical symptoms, is under investigation.
29751604	6	44	theme	prostate	1394:1401	arg1	specimens					1403:1411	rat prostate specimens	1390:1411	rat prostate specimens challenged with Escherichia coli lipopolysaccharide (LPS)	1390:1469	We characterized by means of chromatographic and colorimetric studies the composition of Graminex pollen to better correlate the activity of pollen on immortalized prostate cells (PC3), and in rat prostate specimens challenged with Escherichia coli lipopolysaccharide (LPS).
29751604	1	45	theme	common	222:227	arg1	disease					229:235	a common disease	220:235	a common disease that could be sustained by bacterial or non-bacterial infectious agents	220:307	Prostatitis, a general term describing prostate inflammation, is a common disease that could be sustained by bacterial or non-bacterial infectious agents.
29751604	1	45	theme	common	222:227	arg1	Prostatitis					155:165	Prostatitis	155:165	Prostatitis	155:165	Prostatitis, a general term describing prostate inflammation, is a common disease that could be sustained by bacterial or non-bacterial infectious agents.
29751604	8	46	theme	inflammatory	1762:1773	arg1	components					1775:1784	the inflammatory components	1758:1784	the inflammatory components in the prostate	1758:1800	According to our experimental evidence, Graminex pollen appears to be a promising natural product for the management of the inflammatory components in the prostate.
29751604	4	47	theme	prostate	841:848	arg1	specimens					850:858	rat prostate specimens	837:858	rat prostate specimens	837:858	The aim of the present work was to evaluate the protective effects of Graminex pollen (GraminexTM, Deshler, OH, USA), a commercially available product based on standardized pollen extracts, in rat prostate specimens, ex vivo.
29751604	3	48	theme	Pollen	524:529	arg1	agents					583:588	promising agents	573:588	promising agents	573:588	Pollen extracts have been previously reported as promising agents in managing clinical symptoms related to prostatitis.
29751604	3	48	theme	Pollen	524:529	arg1	extracts					531:538	Pollen extracts	524:538	Pollen extracts	524:538	Pollen extracts have been previously reported as promising agents in managing clinical symptoms related to prostatitis.
29751604	0	49	theme	Colorimetric	35:46	arg1	Analysis					48:55	Colorimetric Analysis	35:55	Colorimetric Analysis	35:55	Graminex Pollen: Phenolic Pattern, Colorimetric Analysis and Protective Effects in Immortalized Prostate Cells (PC3) and Rat Prostate Challenged with LPS.
29751604	4	50	theme	standardized	804:815	arg1	extracts					824:831	standardized pollen extracts	804:831	standardized pollen extracts	804:831	The aim of the present work was to evaluate the protective effects of Graminex pollen (GraminexTM, Deshler, OH, USA), a commercially available product based on standardized pollen extracts, in rat prostate specimens, ex vivo.
29751604	7	51	theme	PC3	1564:1566	arg1	cells					1568:1572	PC3 cells	1564:1572	PC3 cells	1564:1572	We found that Graminex pollen was able to reduce radical oxygen species (ROS) production by PC3 cells and MDA, NFκB mRNA, and PGE₂ levels, in rat prostate specimens.
29751604	7	52	from	specimens	1627:1635	arg1	able					1506:1509	able	1506:1509	able	1506:1509	We found that Graminex pollen was able to reduce radical oxygen species (ROS) production by PC3 cells and MDA, NFκB mRNA, and PGE₂ levels, in rat prostate specimens.
29751604	4	53	theme	work	667:670	arg1	aim					648:650	The aim	644:650	The aim of the present work	644:670	The aim of the present work was to evaluate the protective effects of Graminex pollen (GraminexTM, Deshler, OH, USA), a commercially available product based on standardized pollen extracts, in rat prostate specimens, ex vivo.
29751604	6	54	theme	pollen	1295:1300	arg1	composition					1271:1281	the composition	1267:1281	the composition of Graminex pollen to better correlate the activity of pollen on immortalized prostate cells (PC3), and in rat prostate specimens challenged with Escherichia coli lipopolysaccharide (LPS)	1267:1469	We characterized by means of chromatographic and colorimetric studies the composition of Graminex pollen to better correlate the activity of pollen on immortalized prostate cells (PC3), and in rat prostate specimens challenged with Escherichia coli lipopolysaccharide (LPS).
29751604	1	55	theme	general	170:176	arg1	term					178:181	a general term	168:181	a general term describing prostate inflammation	168:214	Prostatitis, a general term describing prostate inflammation, is a common disease that could be sustained by bacterial or non-bacterial infectious agents.
29751604	1	55	theme	general	170:176	arg1	Prostatitis					155:165	Prostatitis	155:165	Prostatitis	155:165	Prostatitis, a general term describing prostate inflammation, is a common disease that could be sustained by bacterial or non-bacterial infectious agents.
29751604	0	56	theme	Rat	121:123	arg1	Prostate					125:132	Rat Prostate	121:132	Rat Prostate	121:132	Graminex Pollen: Phenolic Pattern, Colorimetric Analysis and Protective Effects in Immortalized Prostate Cells (PC3) and Rat Prostate Challenged with LPS.
29751604	7	57	theme	species	1536:1542	arg1	production					1550:1559	radical oxygen species (ROS) production	1521:1559	radical oxygen species (ROS) production by PC3 cells and MDA	1521:1580	We found that Graminex pollen was able to reduce radical oxygen species (ROS) production by PC3 cells and MDA, NFκB mRNA, and PGE₂ levels, in rat prostate specimens.
29751604	6	58	theme	colorimetric	1246:1257	arg1	studies					1259:1265	chromatographic and colorimetric studies	1226:1265	chromatographic and colorimetric studies	1226:1265	We characterized by means of chromatographic and colorimetric studies the composition of Graminex pollen to better correlate the activity of pollen on immortalized prostate cells (PC3), and in rat prostate specimens challenged with Escherichia coli lipopolysaccharide (LPS).
29751604	8	59	theme	experimental	1655:1666	arg1	evidence					1668:1675	our experimental evidence	1651:1675	our experimental evidence	1651:1675	According to our experimental evidence, Graminex pollen appears to be a promising natural product for the management of the inflammatory components in the prostate.
29751604	7	60	theme	radical	1521:1527	arg1	ROS					1545:1547	ROS	1545:1547	ROS	1545:1547	We found that Graminex pollen was able to reduce radical oxygen species (ROS) production by PC3 cells and MDA, NFκB mRNA, and PGE₂ levels, in rat prostate specimens.
29751604	7	60	theme	radical	1521:1527	arg1	species					1536:1542	radical oxygen species	1521:1542	radical oxygen species (ROS) production by PC3 cells and MDA	1521:1580	We found that Graminex pollen was able to reduce radical oxygen species (ROS) production by PC3 cells and MDA, NFκB mRNA, and PGE₂ levels, in rat prostate specimens.
29751604	4	61	theme	Graminex	714:721	arg1	effects					703:709	the protective effects	688:709	the protective effects of Graminex pollen (GraminexTM, Deshler, OH, USA), a commercially available product based on standardized pollen extracts	688:831	The aim of the present work was to evaluate the protective effects of Graminex pollen (GraminexTM, Deshler, OH, USA), a commercially available product based on standardized pollen extracts, in rat prostate specimens, ex vivo.
29751604	0	62	theme	Protective	61:70	arg1	Effects					72:78	Protective Effects	61:78	Protective Effects	61:78	Graminex Pollen: Phenolic Pattern, Colorimetric Analysis and Protective Effects in Immortalized Prostate Cells (PC3) and Rat Prostate Challenged with LPS.
29751604	5	63	theme	E₂	1017:1018	arg1	reduction					990:998	the reduction	986:998	the reduction of prostaglandin E₂ (PGE₂), nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB), and malondialdehyde (MDA)	986:1123	In this context, we studied the putative mechanism of action of pollen on multiple inflammatory pathways, including the reduction of prostaglandin E₂ (PGE₂), nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB), and malondialdehyde (MDA), whose activities were significantly increased by inflammatory stimuli.
29751604	5	64	theme	B	1083:1083	arg1	cells					1085:1089	activated B cells	1073:1089	activated B cells	1073:1089	In this context, we studied the putative mechanism of action of pollen on multiple inflammatory pathways, including the reduction of prostaglandin E₂ (PGE₂), nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB), and malondialdehyde (MDA), whose activities were significantly increased by inflammatory stimuli.
29751604	7	65	theme	rat	1614:1616	arg1	specimens					1627:1635	rat prostate specimens	1614:1635	rat prostate specimens	1614:1635	We found that Graminex pollen was able to reduce radical oxygen species (ROS) production by PC3 cells and MDA, NFκB mRNA, and PGE₂ levels, in rat prostate specimens.
29751604	0	66	theme	Prostate	96:103	arg1	PC3					112:114	PC3	112:114	PC3	112:114	Graminex Pollen: Phenolic Pattern, Colorimetric Analysis and Protective Effects in Immortalized Prostate Cells (PC3) and Rat Prostate Challenged with LPS.
29751604	0	66	theme	Prostate	96:103	arg1	Cells					105:109	Immortalized Prostate Cells	83:109	Immortalized Prostate Cells (PC3)	83:115	Graminex Pollen: Phenolic Pattern, Colorimetric Analysis and Protective Effects in Immortalized Prostate Cells (PC3) and Rat Prostate Challenged with LPS.
29751604	7	67	theme	Graminex	1486:1493	arg1	pollen					1495:1500	Graminex pollen	1486:1500	Graminex pollen	1486:1500	We found that Graminex pollen was able to reduce radical oxygen species (ROS) production by PC3 cells and MDA, NFκB mRNA, and PGE₂ levels, in rat prostate specimens.
29751604	0	68	from	Analysis	48:55	arg1	PC3					112:114	PC3	112:114	PC3	112:114	Graminex Pollen: Phenolic Pattern, Colorimetric Analysis and Protective Effects in Immortalized Prostate Cells (PC3) and Rat Prostate Challenged with LPS.
29751604	0	68	from	Analysis	48:55	arg1	Cells					105:109	Immortalized Prostate Cells	83:109	Immortalized Prostate Cells (PC3)	83:115	Graminex Pollen: Phenolic Pattern, Colorimetric Analysis and Protective Effects in Immortalized Prostate Cells (PC3) and Rat Prostate Challenged with LPS.
29751604	0	68	from	Analysis	48:55	arg1	Prostate					125:132	Rat Prostate	121:132	Rat Prostate	121:132	Graminex Pollen: Phenolic Pattern, Colorimetric Analysis and Protective Effects in Immortalized Prostate Cells (PC3) and Rat Prostate Challenged with LPS.
29751604	5	69	theme	factor	1036:1041	arg1	kappa-light-chain-enhancer					1043:1068	nuclear factor kappa-light-chain-enhancer	1028:1068	nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB)	1028:1096	In this context, we studied the putative mechanism of action of pollen on multiple inflammatory pathways, including the reduction of prostaglandin E₂ (PGE₂), nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB), and malondialdehyde (MDA), whose activities were significantly increased by inflammatory stimuli.
29751604	5	69	theme	factor	1036:1041	arg1	NFκB					1092:1095	NFκB	1092:1095	NFκB	1092:1095	In this context, we studied the putative mechanism of action of pollen on multiple inflammatory pathways, including the reduction of prostaglandin E₂ (PGE₂), nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB), and malondialdehyde (MDA), whose activities were significantly increased by inflammatory stimuli.
29751604	5	70	theme	action	924:929	arg1	mechanism					911:919	the putative mechanism	898:919	the putative mechanism of action of pollen on multiple inflammatory pathways, including the reduction of prostaglandin E₂ (PGE₂), nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB), and malondialdehyde (MDA), whose activities were significantly increased by inflammatory stimuli	898:1194	In this context, we studied the putative mechanism of action of pollen on multiple inflammatory pathways, including the reduction of prostaglandin E₂ (PGE₂), nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB), and malondialdehyde (MDA), whose activities were significantly increased by inflammatory stimuli.
29751604	7	71	theme	PGE₂	1598:1601	arg1	levels					1603:1608	PGE₂ levels	1598:1608	PGE₂ levels	1598:1608	We found that Graminex pollen was able to reduce radical oxygen species (ROS) production by PC3 cells and MDA, NFκB mRNA, and PGE₂ levels, in rat prostate specimens.
29751604	2	72	from	improvements	465:476	arg1	symptoms					490:497	clinical symptoms	481:497	clinical symptoms	481:497	The efficacy of herbal extracts with antioxidant and anti-inflammatory effects for blunting the burden of inflammation and oxidative stress, with possible improvements in clinical symptoms, is under investigation.
29751604	8	73	theme	natural	1720:1726	arg1	product					1728:1734	a promising natural product	1708:1734	a promising natural product for the management of the inflammatory components in the prostate	1708:1800	According to our experimental evidence, Graminex pollen appears to be a promising natural product for the management of the inflammatory components in the prostate.
29751604	8	73	theme	natural	1720:1726	arg1	pollen					1687:1692	Graminex pollen	1678:1692	Graminex pollen	1678:1692	According to our experimental evidence, Graminex pollen appears to be a promising natural product for the management of the inflammatory components in the prostate.
29751604	2	74	theme	oxidative	433:441	arg1	stress					443:448	oxidative stress	433:448	oxidative stress	433:448	The efficacy of herbal extracts with antioxidant and anti-inflammatory effects for blunting the burden of inflammation and oxidative stress, with possible improvements in clinical symptoms, is under investigation.
29751604	0	75	from	Effects	72:78	arg1	PC3					112:114	PC3	112:114	PC3	112:114	Graminex Pollen: Phenolic Pattern, Colorimetric Analysis and Protective Effects in Immortalized Prostate Cells (PC3) and Rat Prostate Challenged with LPS.
29751604	0	75	from	Effects	72:78	arg1	Cells					105:109	Immortalized Prostate Cells	83:109	Immortalized Prostate Cells (PC3)	83:115	Graminex Pollen: Phenolic Pattern, Colorimetric Analysis and Protective Effects in Immortalized Prostate Cells (PC3) and Rat Prostate Challenged with LPS.
29751604	0	75	from	Effects	72:78	arg1	Prostate					125:132	Rat Prostate	121:132	Rat Prostate	121:132	Graminex Pollen: Phenolic Pattern, Colorimetric Analysis and Protective Effects in Immortalized Prostate Cells (PC3) and Rat Prostate Challenged with LPS.
29751604	0	76	theme	Phenolic	17:24	arg1	Pattern					26:32	Phenolic Pattern	17:32	Phenolic Pattern	17:32	Graminex Pollen: Phenolic Pattern, Colorimetric Analysis and Protective Effects in Immortalized Prostate Cells (PC3) and Rat Prostate Challenged with LPS.
29751604	0	77	dep	Pollen	9:14	arg1	Effects					72:78	Protective Effects	61:78	Protective Effects	61:78	Graminex Pollen: Phenolic Pattern, Colorimetric Analysis and Protective Effects in Immortalized Prostate Cells (PC3) and Rat Prostate Challenged with LPS.
29751604	0	77	dep	Pollen	9:14	arg1	Pattern					26:32	Phenolic Pattern	17:32	Phenolic Pattern	17:32	Graminex Pollen: Phenolic Pattern, Colorimetric Analysis and Protective Effects in Immortalized Prostate Cells (PC3) and Rat Prostate Challenged with LPS.
29751604	0	77	dep	Pollen	9:14	arg1	Analysis					48:55	Colorimetric Analysis	35:55	Colorimetric Analysis	35:55	Graminex Pollen: Phenolic Pattern, Colorimetric Analysis and Protective Effects in Immortalized Prostate Cells (PC3) and Rat Prostate Challenged with LPS.
29751604	7	78	theme	NFκB	1583:1586	arg1	mRNA					1588:1591	NFκB mRNA	1583:1591	NFκB mRNA	1583:1591	We found that Graminex pollen was able to reduce radical oxygen species (ROS) production by PC3 cells and MDA, NFκB mRNA, and PGE₂ levels, in rat prostate specimens.
29751604	2	79	theme	inflammation	416:427	arg1	burden					406:411	the burden	402:411	the burden of inflammation and oxidative stress	402:448	The efficacy of herbal extracts with antioxidant and anti-inflammatory effects for blunting the burden of inflammation and oxidative stress, with possible improvements in clinical symptoms, is under investigation.
29751604	1	80	theme	bacterial	264:272	arg1	agents					302:307	bacterial or non-bacterial infectious agents	264:307	bacterial or non-bacterial infectious agents	264:307	Prostatitis, a general term describing prostate inflammation, is a common disease that could be sustained by bacterial or non-bacterial infectious agents.
29751604	2	81	theme	possible	456:463	arg1	improvements					465:476	possible improvements	456:476	possible improvements in clinical symptoms	456:497	The efficacy of herbal extracts with antioxidant and anti-inflammatory effects for blunting the burden of inflammation and oxidative stress, with possible improvements in clinical symptoms, is under investigation.
29751604	8	82	from	prostate	1793:1800	arg1	management					1744:1753	the management	1740:1753	the management of the inflammatory components in the prostate	1740:1800	According to our experimental evidence, Graminex pollen appears to be a promising natural product for the management of the inflammatory components in the prostate.
29751604	4	83	theme	available	777:785	arg1	product					787:793	a commercially available product	762:793	a commercially available product	762:793	The aim of the present work was to evaluate the protective effects of Graminex pollen (GraminexTM, Deshler, OH, USA), a commercially available product based on standardized pollen extracts, in rat prostate specimens, ex vivo.
29751604	4	83	theme	available	777:785	arg1	Graminex					714:721	Graminex	714:721	Graminex	714:721	The aim of the present work was to evaluate the protective effects of Graminex pollen (GraminexTM, Deshler, OH, USA), a commercially available product based on standardized pollen extracts, in rat prostate specimens, ex vivo.
29751604	6	84	theme	prostate	1361:1368	arg1	cells					1370:1374	immortalized prostate cells	1348:1374	immortalized prostate cells (PC3)	1348:1380	We characterized by means of chromatographic and colorimetric studies the composition of Graminex pollen to better correlate the activity of pollen on immortalized prostate cells (PC3), and in rat prostate specimens challenged with Escherichia coli lipopolysaccharide (LPS).
29751604	6	84	theme	prostate	1361:1368	arg1	PC3					1377:1379	PC3	1377:1379	PC3	1377:1379	We characterized by means of chromatographic and colorimetric studies the composition of Graminex pollen to better correlate the activity of pollen on immortalized prostate cells (PC3), and in rat prostate specimens challenged with Escherichia coli lipopolysaccharide (LPS).
29751604	1	85	theme	non-bacterial	277:289	arg1	agents					302:307	bacterial or non-bacterial infectious agents	264:307	bacterial or non-bacterial infectious agents	264:307	Prostatitis, a general term describing prostate inflammation, is a common disease that could be sustained by bacterial or non-bacterial infectious agents.
29751604	2	86	with	efficacy	314:321	arg1	improvements					465:476	possible improvements	456:476	possible improvements in clinical symptoms	456:497	The efficacy of herbal extracts with antioxidant and anti-inflammatory effects for blunting the burden of inflammation and oxidative stress, with possible improvements in clinical symptoms, is under investigation.
29751604	6	87	theme	coli	1441:1444	arg1	LPS					1466:1468	LPS	1466:1468	LPS	1466:1468	We characterized by means of chromatographic and colorimetric studies the composition of Graminex pollen to better correlate the activity of pollen on immortalized prostate cells (PC3), and in rat prostate specimens challenged with Escherichia coli lipopolysaccharide (LPS).
29751604	6	87	theme	coli	1441:1444	arg1	lipopolysaccharide					1446:1463	Escherichia coli lipopolysaccharide	1429:1463	Escherichia coli lipopolysaccharide (LPS)	1429:1469	We characterized by means of chromatographic and colorimetric studies the composition of Graminex pollen to better correlate the activity of pollen on immortalized prostate cells (PC3), and in rat prostate specimens challenged with Escherichia coli lipopolysaccharide (LPS).
29751604	5	88	theme	pollen	934:939	arg1	action					924:929	action	924:929	action of pollen on multiple inflammatory pathways, including the reduction of prostaglandin E₂ (PGE₂), nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB), and malondialdehyde (MDA), whose activities were significantly increased by inflammatory stimuli	924:1194	In this context, we studied the putative mechanism of action of pollen on multiple inflammatory pathways, including the reduction of prostaglandin E₂ (PGE₂), nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB), and malondialdehyde (MDA), whose activities were significantly increased by inflammatory stimuli.
29751604	5	89	theme	multiple	944:951	arg1	reduction					990:998	the reduction	986:998	the reduction of prostaglandin E₂ (PGE₂), nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB), and malondialdehyde (MDA)	986:1123	In this context, we studied the putative mechanism of action of pollen on multiple inflammatory pathways, including the reduction of prostaglandin E₂ (PGE₂), nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB), and malondialdehyde (MDA), whose activities were significantly increased by inflammatory stimuli.
29751604	5	89	theme	multiple	944:951	arg1	pathways					966:973	multiple inflammatory pathways	944:973	multiple inflammatory pathways	944:973	In this context, we studied the putative mechanism of action of pollen on multiple inflammatory pathways, including the reduction of prostaglandin E₂ (PGE₂), nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB), and malondialdehyde (MDA), whose activities were significantly increased by inflammatory stimuli.
29751604	5	90	from	action	924:929	arg1	reduction					990:998	the reduction	986:998	the reduction of prostaglandin E₂ (PGE₂), nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB), and malondialdehyde (MDA)	986:1123	In this context, we studied the putative mechanism of action of pollen on multiple inflammatory pathways, including the reduction of prostaglandin E₂ (PGE₂), nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB), and malondialdehyde (MDA), whose activities were significantly increased by inflammatory stimuli.
29751604	5	90	from	action	924:929	arg1	pathways					966:973	multiple inflammatory pathways	944:973	multiple inflammatory pathways	944:973	In this context, we studied the putative mechanism of action of pollen on multiple inflammatory pathways, including the reduction of prostaglandin E₂ (PGE₂), nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB), and malondialdehyde (MDA), whose activities were significantly increased by inflammatory stimuli.
29751604	5	91	theme	inflammatory	1175:1186	arg1	stimuli					1188:1194	inflammatory stimuli	1175:1194	inflammatory stimuli	1175:1194	In this context, we studied the putative mechanism of action of pollen on multiple inflammatory pathways, including the reduction of prostaglandin E₂ (PGE₂), nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB), and malondialdehyde (MDA), whose activities were significantly increased by inflammatory stimuli.
31282158	1	0	theme	rice	221:224	arg1	GR2E					232:235	golden rice event GR2E	214:235	golden rice event GR2E	214:235	Compositional analyses were performed on samples of rice grain, straw, and derived bran obtained from golden rice event GR2E and near-isogenic control PSBRc82 rice grown at four locations in the Philippines during 2015 and 2016.
31282158	2	1	theme	fatty	447:451	arg1	acids					453:457	fatty acids	447:457	fatty acids	447:457	Grain samples were analyzed for key nutritional components, including proximates, fiber, polysaccharides, fatty acids, amino acids, minerals, vitamins, and antinutrients.
31282158	1	2	from	locations	290:298	arg1	Philippines					307:317	the Philippines	303:317	the Philippines during 2015 and 2016	303:338	Compositional analyses were performed on samples of rice grain, straw, and derived bran obtained from golden rice event GR2E and near-isogenic control PSBRc82 rice grown at four locations in the Philippines during 2015 and 2016.
31282158	5	3	theme	natural	846:852	arg1	variability					854:864	natural variability	846:864	natural variability of those components in conventional rice varieties with a history of safe consumption	846:950	Except for β-carotene and related carotenoids, the compositional parameters of GR2E rice were within the range of natural variability of those components in conventional rice varieties with a history of safe consumption.
31282158	1	4	theme	event	226:230	arg1	GR2E					232:235	golden rice event GR2E	214:235	golden rice event GR2E	214:235	Compositional analyses were performed on samples of rice grain, straw, and derived bran obtained from golden rice event GR2E and near-isogenic control PSBRc82 rice grown at four locations in the Philippines during 2015 and 2016.
31282158	4	5	theme	provitamin	693:702	arg1	carotenoids					706:716	other provitamin A carotenoids	687:716	other provitamin A carotenoids in the grain	687:729	The only biologically meaningful difference between GR2E and control rice was in levels of β-carotene and other provitamin A carotenoids in the grain.
31282158	6	6	theme	vitamin	1090:1096	arg1	A					1098:1098	vitamin A	1090:1098	vitamin A	1090:1098	Mean provitamin A concentrations in milled rice of GR2E can contribute up to 89-113% and 57-99% of the estimated average requirement for vitamin A for preschool children in Bangladesh and the Philippines, respectively.
31282158	6	7	theme	preschool	1104:1112	arg1	children					1114:1121	preschool children	1104:1121	preschool children in Bangladesh	1104:1135	Mean provitamin A concentrations in milled rice of GR2E can contribute up to 89-113% and 57-99% of the estimated average requirement for vitamin A for preschool children in Bangladesh and the Philippines, respectively.
31282158	5	8	with	varieties	907:915	arg1	history					924:930	a history	922:930	a history of safe consumption	922:950	Except for β-carotene and related carotenoids, the compositional parameters of GR2E rice were within the range of natural variability of those components in conventional rice varieties with a history of safe consumption.
31282158	0	9	theme	Conventional	93:104	arg1	Rice					106:109	Conventional Rice	93:109	Conventional Rice	93:109	Compositional Analysis of Genetically Engineered GR2E "Golden Rice" in Comparison to That of Conventional Rice.
31282158	6	10	theme	estimated	1056:1064	arg1	requirement					1074:1084	the estimated average requirement	1052:1084	the estimated average requirement for vitamin A for preschool children in Bangladesh and the Philippines	1052:1155	Mean provitamin A concentrations in milled rice of GR2E can contribute up to 89-113% and 57-99% of the estimated average requirement for vitamin A for preschool children in Bangladesh and the Philippines, respectively.
31282158	5	11	theme	compositional	783:795	arg1	parameters					797:806	the compositional parameters	779:806	the compositional parameters of GR2E rice	779:819	Except for β-carotene and related carotenoids, the compositional parameters of GR2E rice were within the range of natural variability of those components in conventional rice varieties with a history of safe consumption.
31282158	4	12	from	carotenoids	706:716	arg1	grain					725:729	the grain	721:729	the grain	721:729	The only biologically meaningful difference between GR2E and control rice was in levels of β-carotene and other provitamin A carotenoids in the grain.
31282158	5	13	theme	GR2E	811:814	arg1	rice					816:819	GR2E rice	811:819	GR2E rice	811:819	Except for β-carotene and related carotenoids, the compositional parameters of GR2E rice were within the range of natural variability of those components in conventional rice varieties with a history of safe consumption.
31282158	6	14	theme	Mean	953:956	arg1	concentrations					971:984	Mean provitamin A concentrations	953:984	Mean provitamin A concentrations in milled rice of GR2E	953:1007	Mean provitamin A concentrations in milled rice of GR2E can contribute up to 89-113% and 57-99% of the estimated average requirement for vitamin A for preschool children in Bangladesh and the Philippines, respectively.
31282158	6	15	theme	requirement	1074:1084	arg1	%					1036:1036	89-113%	1030:1036	89-113%	1030:1036	Mean provitamin A concentrations in milled rice of GR2E can contribute up to 89-113% and 57-99% of the estimated average requirement for vitamin A for preschool children in Bangladesh and the Philippines, respectively.
31282158	6	15	theme	requirement	1074:1084	arg1	%					1047:1047	57-99%	1042:1047	57-99% of the estimated average requirement for vitamin A for preschool children in Bangladesh and the Philippines	1042:1155	Mean provitamin A concentrations in milled rice of GR2E can contribute up to 89-113% and 57-99% of the estimated average requirement for vitamin A for preschool children in Bangladesh and the Philippines, respectively.
31282158	6	15	theme	requirement	1074:1084	arg1	requirement					1074:1084	the estimated average requirement	1052:1084	the estimated average requirement for vitamin A for preschool children in Bangladesh and the Philippines	1052:1155	Mean provitamin A concentrations in milled rice of GR2E can contribute up to 89-113% and 57-99% of the estimated average requirement for vitamin A for preschool children in Bangladesh and the Philippines, respectively.
31282158	5	16	theme	related	758:764	arg1	carotenoids					766:776	β-carotene and related carotenoids	743:776	β-carotene and related carotenoids	743:776	Except for β-carotene and related carotenoids, the compositional parameters of GR2E rice were within the range of natural variability of those components in conventional rice varieties with a history of safe consumption.
31282158	6	17	from	children	1114:1121	arg1	Bangladesh					1126:1135	Bangladesh	1126:1135	Bangladesh	1126:1135	Mean provitamin A concentrations in milled rice of GR2E can contribute up to 89-113% and 57-99% of the estimated average requirement for vitamin A for preschool children in Bangladesh and the Philippines, respectively.
31282158	1	18	theme	rice	164:167	arg1	grain					169:173	rice grain	164:173	rice grain	164:173	Compositional analyses were performed on samples of rice grain, straw, and derived bran obtained from golden rice event GR2E and near-isogenic control PSBRc82 rice grown at four locations in the Philippines during 2015 and 2016.
31282158	5	19	from	varieties	907:915	arg1	variability					854:864	natural variability	846:864	natural variability of those components in conventional rice varieties with a history of safe consumption	846:950	Except for β-carotene and related carotenoids, the compositional parameters of GR2E rice were within the range of natural variability of those components in conventional rice varieties with a history of safe consumption.
31282158	5	20	from	components	875:884	arg1	varieties					907:915	conventional rice varieties	889:915	conventional rice varieties with a history of safe consumption	889:950	Except for β-carotene and related carotenoids, the compositional parameters of GR2E rice were within the range of natural variability of those components in conventional rice varieties with a history of safe consumption.
31282158	4	21	theme	other	687:691	arg1	carotenoids					706:716	other provitamin A carotenoids	687:716	other provitamin A carotenoids in the grain	687:729	The only biologically meaningful difference between GR2E and control rice was in levels of β-carotene and other provitamin A carotenoids in the grain.
31282158	1	22	theme	grain	169:173	arg1	samples					153:159	samples	153:159	samples of rice grain, straw, and derived bran obtained from golden rice event GR2E and near-isogenic control PSBRc82 rice grown at four locations in the Philippines during 2015 and 2016	153:338	Compositional analyses were performed on samples of rice grain, straw, and derived bran obtained from golden rice event GR2E and near-isogenic control PSBRc82 rice grown at four locations in the Philippines during 2015 and 2016.
31282158	0	23	theme	Compositional	0:12	arg1	Analysis					14:21	Compositional Analysis	0:21	Compositional Analysis of Genetically Engineered GR2E	0:52	Compositional Analysis of Genetically Engineered GR2E "Golden Rice" in Comparison to That of Conventional Rice.
31282158	1	24	theme	near-isogenic	241:253	arg1	rice					271:274	near-isogenic control PSBRc82 rice	241:274	near-isogenic control PSBRc82 rice	241:274	Compositional analyses were performed on samples of rice grain, straw, and derived bran obtained from golden rice event GR2E and near-isogenic control PSBRc82 rice grown at four locations in the Philippines during 2015 and 2016.
31282158	5	25	from	variability	854:864	arg1	varieties					907:915	conventional rice varieties	889:915	conventional rice varieties with a history of safe consumption	889:950	Except for β-carotene and related carotenoids, the compositional parameters of GR2E rice were within the range of natural variability of those components in conventional rice varieties with a history of safe consumption.
31282158	3	26	theme	straw	523:527	arg1	Samples					512:518	Samples	512:518	Samples of straw and bran	512:536	Samples of straw and bran were analyzed for proximates and minerals.
31282158	1	27	link	derived	187:193	arg1	bran					195:198	derived bran	187:198	derived bran	187:198	Compositional analyses were performed on samples of rice grain, straw, and derived bran obtained from golden rice event GR2E and near-isogenic control PSBRc82 rice grown at four locations in the Philippines during 2015 and 2016.
31282158	1	28	theme	control	255:261	arg1	rice					271:274	near-isogenic control PSBRc82 rice	241:274	near-isogenic control PSBRc82 rice	241:274	Compositional analyses were performed on samples of rice grain, straw, and derived bran obtained from golden rice event GR2E and near-isogenic control PSBRc82 rice grown at four locations in the Philippines during 2015 and 2016.
31282158	4	29	theme	A	704:704	arg1	carotenoids					706:716	other provitamin A carotenoids	687:716	other provitamin A carotenoids in the grain	687:729	The only biologically meaningful difference between GR2E and control rice was in levels of β-carotene and other provitamin A carotenoids in the grain.
31282158	1	30	theme	straw	176:180	arg1	samples					153:159	samples	153:159	samples of rice grain, straw, and derived bran obtained from golden rice event GR2E and near-isogenic control PSBRc82 rice grown at four locations in the Philippines during 2015 and 2016	153:338	Compositional analyses were performed on samples of rice grain, straw, and derived bran obtained from golden rice event GR2E and near-isogenic control PSBRc82 rice grown at four locations in the Philippines during 2015 and 2016.
31282158	5	31	theme	conventional	889:900	arg1	varieties					907:915	conventional rice varieties	889:915	conventional rice varieties with a history of safe consumption	889:950	Except for β-carotene and related carotenoids, the compositional parameters of GR2E rice were within the range of natural variability of those components in conventional rice varieties with a history of safe consumption.
31282158	1	32	theme	PSBRc82	263:269	arg1	rice					271:274	near-isogenic control PSBRc82 rice	241:274	near-isogenic control PSBRc82 rice	241:274	Compositional analyses were performed on samples of rice grain, straw, and derived bran obtained from golden rice event GR2E and near-isogenic control PSBRc82 rice grown at four locations in the Philippines during 2015 and 2016.
31282158	0	33	dep	Rice	62:65	arg1	Analysis					14:21	Compositional Analysis	0:21	Compositional Analysis of Genetically Engineered GR2E	0:52	Compositional Analysis of Genetically Engineered GR2E "Golden Rice" in Comparison to That of Conventional Rice.
31282158	2	34	theme	nutritional	377:387	arg1	minerals					473:480	minerals	473:480	minerals	473:480	Grain samples were analyzed for key nutritional components, including proximates, fiber, polysaccharides, fatty acids, amino acids, minerals, vitamins, and antinutrients.
31282158	2	34	theme	nutritional	377:387	arg1	proximates					411:420	proximates	411:420	proximates	411:420	Grain samples were analyzed for key nutritional components, including proximates, fiber, polysaccharides, fatty acids, amino acids, minerals, vitamins, and antinutrients.
31282158	2	34	theme	nutritional	377:387	arg1	acids					453:457	fatty acids	447:457	fatty acids	447:457	Grain samples were analyzed for key nutritional components, including proximates, fiber, polysaccharides, fatty acids, amino acids, minerals, vitamins, and antinutrients.
31282158	2	34	theme	nutritional	377:387	arg1	vitamins					483:490	vitamins	483:490	vitamins	483:490	Grain samples were analyzed for key nutritional components, including proximates, fiber, polysaccharides, fatty acids, amino acids, minerals, vitamins, and antinutrients.
31282158	2	34	theme	nutritional	377:387	arg1	fiber					423:427	fiber	423:427	fiber	423:427	Grain samples were analyzed for key nutritional components, including proximates, fiber, polysaccharides, fatty acids, amino acids, minerals, vitamins, and antinutrients.
31282158	2	34	theme	nutritional	377:387	arg1	components					389:398	key nutritional components	373:398	key nutritional components	373:398	Grain samples were analyzed for key nutritional components, including proximates, fiber, polysaccharides, fatty acids, amino acids, minerals, vitamins, and antinutrients.
31282158	2	34	theme	nutritional	377:387	arg1	antinutrients					497:509	antinutrients	497:509	antinutrients	497:509	Grain samples were analyzed for key nutritional components, including proximates, fiber, polysaccharides, fatty acids, amino acids, minerals, vitamins, and antinutrients.
31282158	2	34	theme	nutritional	377:387	arg1	polysaccharides					430:444	polysaccharides	430:444	polysaccharides	430:444	Grain samples were analyzed for key nutritional components, including proximates, fiber, polysaccharides, fatty acids, amino acids, minerals, vitamins, and antinutrients.
31282158	2	34	theme	nutritional	377:387	arg1	acids					466:470	amino acids	460:470	amino acids	460:470	Grain samples were analyzed for key nutritional components, including proximates, fiber, polysaccharides, fatty acids, amino acids, minerals, vitamins, and antinutrients.
31282158	0	35	theme	GR2E	49:52	arg1	Analysis					14:21	Compositional Analysis	0:21	Compositional Analysis of Genetically Engineered GR2E	0:52	Compositional Analysis of Genetically Engineered GR2E "Golden Rice" in Comparison to That of Conventional Rice.
31282158	2	36	theme	Grain	341:345	arg1	samples					347:353	Grain samples	341:353	Grain samples	341:353	Grain samples were analyzed for key nutritional components, including proximates, fiber, polysaccharides, fatty acids, amino acids, minerals, vitamins, and antinutrients.
31282158	2	37	theme	key	373:375	arg1	minerals					473:480	minerals	473:480	minerals	473:480	Grain samples were analyzed for key nutritional components, including proximates, fiber, polysaccharides, fatty acids, amino acids, minerals, vitamins, and antinutrients.
31282158	2	37	theme	key	373:375	arg1	proximates					411:420	proximates	411:420	proximates	411:420	Grain samples were analyzed for key nutritional components, including proximates, fiber, polysaccharides, fatty acids, amino acids, minerals, vitamins, and antinutrients.
31282158	2	37	theme	key	373:375	arg1	acids					453:457	fatty acids	447:457	fatty acids	447:457	Grain samples were analyzed for key nutritional components, including proximates, fiber, polysaccharides, fatty acids, amino acids, minerals, vitamins, and antinutrients.
31282158	2	37	theme	key	373:375	arg1	vitamins					483:490	vitamins	483:490	vitamins	483:490	Grain samples were analyzed for key nutritional components, including proximates, fiber, polysaccharides, fatty acids, amino acids, minerals, vitamins, and antinutrients.
31282158	2	37	theme	key	373:375	arg1	fiber					423:427	fiber	423:427	fiber	423:427	Grain samples were analyzed for key nutritional components, including proximates, fiber, polysaccharides, fatty acids, amino acids, minerals, vitamins, and antinutrients.
31282158	2	37	theme	key	373:375	arg1	components					389:398	key nutritional components	373:398	key nutritional components	373:398	Grain samples were analyzed for key nutritional components, including proximates, fiber, polysaccharides, fatty acids, amino acids, minerals, vitamins, and antinutrients.
31282158	2	37	theme	key	373:375	arg1	antinutrients					497:509	antinutrients	497:509	antinutrients	497:509	Grain samples were analyzed for key nutritional components, including proximates, fiber, polysaccharides, fatty acids, amino acids, minerals, vitamins, and antinutrients.
31282158	2	37	theme	key	373:375	arg1	polysaccharides					430:444	polysaccharides	430:444	polysaccharides	430:444	Grain samples were analyzed for key nutritional components, including proximates, fiber, polysaccharides, fatty acids, amino acids, minerals, vitamins, and antinutrients.
31282158	2	37	theme	key	373:375	arg1	acids					466:470	amino acids	460:470	amino acids	460:470	Grain samples were analyzed for key nutritional components, including proximates, fiber, polysaccharides, fatty acids, amino acids, minerals, vitamins, and antinutrients.
31282158	0	38	theme	Engineered	38:47	arg1	GR2E					49:52	Genetically Engineered GR2E	26:52	Genetically Engineered GR2E	26:52	Compositional Analysis of Genetically Engineered GR2E "Golden Rice" in Comparison to That of Conventional Rice.
31282158	5	39	theme	rice	816:819	arg1	parameters					797:806	the compositional parameters	779:806	the compositional parameters of GR2E rice	779:819	Except for β-carotene and related carotenoids, the compositional parameters of GR2E rice were within the range of natural variability of those components in conventional rice varieties with a history of safe consumption.
31282158	6	40	from	Philippines	1145:1155	arg1	Bangladesh					1126:1135	Bangladesh	1126:1135	Bangladesh	1126:1135	Mean provitamin A concentrations in milled rice of GR2E can contribute up to 89-113% and 57-99% of the estimated average requirement for vitamin A for preschool children in Bangladesh and the Philippines, respectively.
31282158	1	41	theme	derived	187:193	arg1	bran					195:198	derived bran	187:198	derived bran	187:198	Compositional analyses were performed on samples of rice grain, straw, and derived bran obtained from golden rice event GR2E and near-isogenic control PSBRc82 rice grown at four locations in the Philippines during 2015 and 2016.
31282158	5	42	theme	variability	854:864	arg1	range					837:841	the range	833:841	the range of natural variability of those components in conventional rice varieties with a history of safe consumption	833:950	Except for β-carotene and related carotenoids, the compositional parameters of GR2E rice were within the range of natural variability of those components in conventional rice varieties with a history of safe consumption.
31282158	5	43	theme	β-carotene	743:752	arg1	carotenoids					766:776	β-carotene and related carotenoids	743:776	β-carotene and related carotenoids	743:776	Except for β-carotene and related carotenoids, the compositional parameters of GR2E rice were within the range of natural variability of those components in conventional rice varieties with a history of safe consumption.
31282158	3	44	theme	bran	533:536	arg1	Samples					512:518	Samples	512:518	Samples of straw and bran	512:536	Samples of straw and bran were analyzed for proximates and minerals.
31282158	5	45	theme	safe	935:938	arg1	consumption					940:950	safe consumption	935:950	safe consumption	935:950	Except for β-carotene and related carotenoids, the compositional parameters of GR2E rice were within the range of natural variability of those components in conventional rice varieties with a history of safe consumption.
31282158	1	46	theme	Compositional	112:124	arg1	analyses					126:133	Compositional analyses	112:133	Compositional analyses	112:133	Compositional analyses were performed on samples of rice grain, straw, and derived bran obtained from golden rice event GR2E and near-isogenic control PSBRc82 rice grown at four locations in the Philippines during 2015 and 2016.
31282158	5	47	theme	rice	902:905	arg1	varieties					907:915	conventional rice varieties	889:915	conventional rice varieties with a history of safe consumption	889:950	Except for β-carotene and related carotenoids, the compositional parameters of GR2E rice were within the range of natural variability of those components in conventional rice varieties with a history of safe consumption.
31282158	1	48	theme	bran	195:198	arg1	samples					153:159	samples	153:159	samples of rice grain, straw, and derived bran obtained from golden rice event GR2E and near-isogenic control PSBRc82 rice grown at four locations in the Philippines during 2015 and 2016	153:338	Compositional analyses were performed on samples of rice grain, straw, and derived bran obtained from golden rice event GR2E and near-isogenic control PSBRc82 rice grown at four locations in the Philippines during 2015 and 2016.
31282158	5	49	theme	consumption	940:950	arg1	history					924:930	a history	922:930	a history of safe consumption	922:950	Except for β-carotene and related carotenoids, the compositional parameters of GR2E rice were within the range of natural variability of those components in conventional rice varieties with a history of safe consumption.
31282158	5	50	theme	components	875:884	arg1	variability					854:864	natural variability	846:864	natural variability of those components in conventional rice varieties with a history of safe consumption	846:950	Except for β-carotene and related carotenoids, the compositional parameters of GR2E rice were within the range of natural variability of those components in conventional rice varieties with a history of safe consumption.
31282158	4	51	from	levels	662:667	arg1	grain					725:729	the grain	721:729	the grain	721:729	The only biologically meaningful difference between GR2E and control rice was in levels of β-carotene and other provitamin A carotenoids in the grain.
31282158	4	52	theme	β-carotene	672:681	arg1	levels					662:667	levels	662:667	levels of β-carotene	662:681	The only biologically meaningful difference between GR2E and control rice was in levels of β-carotene and other provitamin A carotenoids in the grain.
31282158	4	52	theme	β-carotene	672:681	arg1	carotenoids					706:716	other provitamin A carotenoids	687:716	other provitamin A carotenoids in the grain	687:729	The only biologically meaningful difference between GR2E and control rice was in levels of β-carotene and other provitamin A carotenoids in the grain.
31282158	2	53	theme	amino	460:464	arg1	acids					466:470	amino acids	460:470	amino acids	460:470	Grain samples were analyzed for key nutritional components, including proximates, fiber, polysaccharides, fatty acids, amino acids, minerals, vitamins, and antinutrients.
31282158	6	54	theme	milled	989:994	arg1	rice					996:999	milled rice	989:999	milled rice of GR2E	989:1007	Mean provitamin A concentrations in milled rice of GR2E can contribute up to 89-113% and 57-99% of the estimated average requirement for vitamin A for preschool children in Bangladesh and the Philippines, respectively.
31282158	6	55	from	concentrations	971:984	arg1	rice					996:999	milled rice	989:999	milled rice of GR2E	989:1007	Mean provitamin A concentrations in milled rice of GR2E can contribute up to 89-113% and 57-99% of the estimated average requirement for vitamin A for preschool children in Bangladesh and the Philippines, respectively.
31282158	6	56	theme	GR2E	1004:1007	arg1	rice					996:999	milled rice	989:999	milled rice of GR2E	989:1007	Mean provitamin A concentrations in milled rice of GR2E can contribute up to 89-113% and 57-99% of the estimated average requirement for vitamin A for preschool children in Bangladesh and the Philippines, respectively.
31282158	4	57	theme	control	642:648	arg1	rice					650:653	control rice	642:653	control rice	642:653	The only biologically meaningful difference between GR2E and control rice was in levels of β-carotene and other provitamin A carotenoids in the grain.
31282158	4	58	theme	meaningful	603:612	arg1	difference					614:623	The only biologically meaningful difference	581:623	The only biologically meaningful difference between GR2E and control rice	581:653	The only biologically meaningful difference between GR2E and control rice was in levels of β-carotene and other provitamin A carotenoids in the grain.
31282158	6	59	theme	A	969:969	arg1	concentrations					971:984	Mean provitamin A concentrations	953:984	Mean provitamin A concentrations in milled rice of GR2E	953:1007	Mean provitamin A concentrations in milled rice of GR2E can contribute up to 89-113% and 57-99% of the estimated average requirement for vitamin A for preschool children in Bangladesh and the Philippines, respectively.
31282158	6	60	theme	average	1066:1072	arg1	requirement					1074:1084	the estimated average requirement	1052:1084	the estimated average requirement for vitamin A for preschool children in Bangladesh and the Philippines	1052:1155	Mean provitamin A concentrations in milled rice of GR2E can contribute up to 89-113% and 57-99% of the estimated average requirement for vitamin A for preschool children in Bangladesh and the Philippines, respectively.
31282158	6	61	theme	provitamin	958:967	arg1	concentrations					971:984	Mean provitamin A concentrations	953:984	Mean provitamin A concentrations in milled rice of GR2E	953:1007	Mean provitamin A concentrations in milled rice of GR2E can contribute up to 89-113% and 57-99% of the estimated average requirement for vitamin A for preschool children in Bangladesh and the Philippines, respectively.
31282158	1	62	theme	golden	214:219	arg1	GR2E					232:235	golden rice event GR2E	214:235	golden rice event GR2E	214:235	Compositional analyses were performed on samples of rice grain, straw, and derived bran obtained from golden rice event GR2E and near-isogenic control PSBRc82 rice grown at four locations in the Philippines during 2015 and 2016.
30900363	5	0	theme	non-woven	856:864	arg1	chitosan-gelatin-poly-l-lactide					811:841	chitosan-gelatin-poly-l-lactide	811:841	chitosan-gelatin-poly-l-lactide	811:841	RESULTS With the use of a copolymer of chitosan-gelatin-poly-l-lactide, a bilayered non-woven scaffold was generated and characterized.
30900363	5	0	theme	non-woven	856:864	arg1	scaffold					866:873	a bilayered non-woven scaffold	844:873	a bilayered non-woven scaffold	844:873	RESULTS With the use of a copolymer of chitosan-gelatin-poly-l-lactide, a bilayered non-woven scaffold was generated and characterized.
30900363	7	1	theme	pseudostratified	1107:1122	arg1	layer					1135:1139	pseudostratified epithelial layer	1107:1139	pseudostratified epithelial layer	1107:1139	The basal airway epithelial cells differentiated into ciliary and goblet cells and formed pseudostratified epithelial layer on the surface of the scaffold.
30900363	2	2	theme	cultivation	387:397	arg1	conditions					399:408	the cultivation conditions	383:408	the cultivation conditions	383:408	Here, we have investigated the cultivation conditions and structural features of the biodegradable non-woven material in order to obtain a well-differentiated human airway epithelium.
30900363	10	3	theme	nanofibres	1397:1406	arg1	parameters					1383:1392	The structural parameters	1368:1392	CONCLUSIONS The structural parameters of nanofibres	1356:1406	CONCLUSIONS The structural parameters of nanofibres should be selected experimentally depending on polymer composition.
30900363	4	4	theme	scaffold	653:660	arg1	efficiency					635:644	The efficiency	631:644	The efficiency of the scaffold	631:660	The efficiency of the scaffold has been evaluated using MTT cell proliferation assay, histology, immunofluorescence and electron microscopy.
30900363	11	5	theme	well-differentiated	1522:1540	arg1	equivalent					1542:1551	the well-differentiated equivalent	1518:1551	the well-differentiated equivalent of respiratory epithelium on non-woven scaffold	1518:1599	The major challenges on the way to obtain the well-differentiated equivalent of respiratory epithelium on non-woven scaffold include the following: the balance between scaffold permeability and thickness, proper combination of synthetic and natural components, and culture conditions sufficient for co-culturing of airway epithelial cells and fibroblasts.
30900363	11	6	theme	fibroblasts	1819:1829	arg1	co-culturing					1775:1786	co-culturing	1775:1786	co-culturing of airway epithelial cells and fibroblasts	1775:1829	The major challenges on the way to obtain the well-differentiated equivalent of respiratory epithelium on non-woven scaffold include the following: the balance between scaffold permeability and thickness, proper combination of synthetic and natural components, and culture conditions sufficient for co-culturing of airway epithelial cells and fibroblasts.
30900363	6	7	theme	structural	920:929	arg1	parameters					931:940	The optimal structural parameters	908:940	The optimal structural parameters of both layers for cell proliferation and differentiation	908:998	The optimal structural parameters of both layers for cell proliferation and differentiation were determined.
30900363	3	8	theme	bilayered	566:574	arg1	scaffold					576:583	The bilayered scaffold	562:583	MATERIALS AND METHODS The bilayered scaffold	540:583	MATERIALS AND METHODS The bilayered scaffold was fabricated by electrospinning technology.
30900363	12	9	theme	diffusion	1879:1887	arg1	lack					1871:1874	the lack	1867:1874	the lack of diffusion	1867:1887	For generation of skin equivalent, the lack of diffusion is not so critical as for pseudostratified airway epithelium.
30900363	12	9	theme	diffusion	1879:1887	arg1	critical					1899:1906	critical	1899:1906	critical	1899:1906	For generation of skin equivalent, the lack of diffusion is not so critical as for pseudostratified airway epithelium.
30900363	5	10	theme	copolymer	798:806	arg1	use					789:791	the use	785:791	the use of a copolymer of chitosan-gelatin-poly-l-lactide, a bilayered non-woven scaffold	785:873	RESULTS With the use of a copolymer of chitosan-gelatin-poly-l-lactide, a bilayered non-woven scaffold was generated and characterized.
30900363	6	11	theme	optimal	912:918	arg1	parameters					931:940	The optimal structural parameters	908:940	The optimal structural parameters of both layers for cell proliferation and differentiation	908:998	The optimal structural parameters of both layers for cell proliferation and differentiation were determined.
30900363	7	12	dep	ciliary	1071:1077	arg1	cells					1090:1094	cells	1090:1094	cells	1090:1094	The basal airway epithelial cells differentiated into ciliary and goblet cells and formed pseudostratified epithelial layer on the surface of the scaffold.
30900363	11	13	theme	cells	1809:1813	arg1	co-culturing					1775:1786	co-culturing	1775:1786	co-culturing of airway epithelial cells and fibroblasts	1775:1829	The major challenges on the way to obtain the well-differentiated equivalent of respiratory epithelium on non-woven scaffold include the following: the balance between scaffold permeability and thickness, proper combination of synthetic and natural components, and culture conditions sufficient for co-culturing of airway epithelial cells and fibroblasts.
30900363	11	14	theme	epithelial	1798:1807	arg1	cells					1809:1813	airway epithelial cells	1791:1813	airway epithelial cells	1791:1813	The major challenges on the way to obtain the well-differentiated equivalent of respiratory epithelium on non-woven scaffold include the following: the balance between scaffold permeability and thickness, proper combination of synthetic and natural components, and culture conditions sufficient for co-culturing of airway epithelial cells and fibroblasts.
30900363	3	15	dep	MATERIALS	540:548	arg1	scaffold					576:583	The bilayered scaffold	562:583	MATERIALS AND METHODS The bilayered scaffold	540:583	MATERIALS AND METHODS The bilayered scaffold was fabricated by electrospinning technology.
30900363	12	16	theme	skin	1850:1853	arg1	equivalent					1855:1864	skin equivalent	1850:1864	skin equivalent	1850:1864	For generation of skin equivalent, the lack of diffusion is not so critical as for pseudostratified airway epithelium.
30900363	5	17	theme	chitosan-gelatin-poly-l-lactide	811:841	arg1	copolymer					798:806	a copolymer	796:806	a copolymer of chitosan-gelatin-poly-l-lactide, a bilayered non-woven scaffold	796:873	RESULTS With the use of a copolymer of chitosan-gelatin-poly-l-lactide, a bilayered non-woven scaffold was generated and characterized.
30900363	4	18	theme	proliferation	696:708	arg1	assay					710:714	MTT cell proliferation assay	687:714	MTT cell proliferation assay	687:714	The efficiency of the scaffold has been evaluated using MTT cell proliferation assay, histology, immunofluorescence and electron microscopy.
30900363	5	19	with	RESULTS	772:778	arg1	use					789:791	the use	785:791	the use of a copolymer of chitosan-gelatin-poly-l-lactide, a bilayered non-woven scaffold	785:873	RESULTS With the use of a copolymer of chitosan-gelatin-poly-l-lactide, a bilayered non-woven scaffold was generated and characterized.
30900363	11	20	theme	epithelium	1568:1577	arg1	equivalent					1542:1551	the well-differentiated equivalent	1518:1551	the well-differentiated equivalent of respiratory epithelium on non-woven scaffold	1518:1599	The major challenges on the way to obtain the well-differentiated equivalent of respiratory epithelium on non-woven scaffold include the following: the balance between scaffold permeability and thickness, proper combination of synthetic and natural components, and culture conditions sufficient for co-culturing of airway epithelial cells and fibroblasts.
30900363	11	21	from	challenges	1486:1495	arg1	way					1504:1506	the way	1500:1506	the way to obtain the well-differentiated equivalent of respiratory epithelium on non-woven scaffold	1500:1599	The major challenges on the way to obtain the well-differentiated equivalent of respiratory epithelium on non-woven scaffold include the following: the balance between scaffold permeability and thickness, proper combination of synthetic and natural components, and culture conditions sufficient for co-culturing of airway epithelial cells and fibroblasts.
30900363	11	22	dep	way	1504:1506	arg1	obtain					1511:1516	obtain	1511:1516	to obtain the well-differentiated equivalent of respiratory epithelium on non-woven scaffold	1508:1599	The major challenges on the way to obtain the well-differentiated equivalent of respiratory epithelium on non-woven scaffold include the following: the balance between scaffold permeability and thickness, proper combination of synthetic and natural components, and culture conditions sufficient for co-culturing of airway epithelial cells and fibroblasts.
30900363	10	23	dep	CONCLUSIONS	1356:1366	arg1	parameters					1383:1392	The structural parameters	1368:1392	CONCLUSIONS The structural parameters of nanofibres	1356:1406	CONCLUSIONS The structural parameters of nanofibres should be selected experimentally depending on polymer composition.
30900363	10	24	theme	structural	1372:1381	arg1	parameters					1383:1392	The structural parameters	1368:1392	CONCLUSIONS The structural parameters of nanofibres	1356:1406	CONCLUSIONS The structural parameters of nanofibres should be selected experimentally depending on polymer composition.
30900363	11	25	theme	respiratory	1556:1566	arg1	epithelium					1568:1577	respiratory epithelium	1556:1577	respiratory epithelium	1556:1577	The major challenges on the way to obtain the well-differentiated equivalent of respiratory epithelium on non-woven scaffold include the following: the balance between scaffold permeability and thickness, proper combination of synthetic and natural components, and culture conditions sufficient for co-culturing of airway epithelial cells and fibroblasts.
30900363	9	26	theme	epithelium	1330:1339	arg1	types					1321:1325	both types	1316:1325	both types of epithelium	1316:1339	A comparative analysis of growth and differentiation for both types of epithelium was performed.
30900363	12	27	theme	equivalent	1855:1864	arg1	generation					1836:1845	generation	1836:1845	generation of skin equivalent	1836:1864	For generation of skin equivalent, the lack of diffusion is not so critical as for pseudostratified airway epithelium.
30900363	4	28	theme	electron	751:758	arg1	microscopy					760:769	electron microscopy	751:769	electron microscopy	751:769	The efficiency of the scaffold has been evaluated using MTT cell proliferation assay, histology, immunofluorescence and electron microscopy.
30900363	1	29	theme	tissue	309:314	arg1	formation					316:324	tissue formation	309:324	tissue formation	309:324	OBJECTIVES The conversion of tissue engineering into a routine clinical tool cannot be achieved without a deep understanding of the interaction between cells and scaffolds during the process of tissue formation in an artificial environment.
30900363	12	30	theme	airway	1932:1937	arg1	epithelium					1939:1948	pseudostratified airway epithelium	1915:1948	pseudostratified airway epithelium	1915:1948	For generation of skin equivalent, the lack of diffusion is not so critical as for pseudostratified airway epithelium.
30900363	1	31	theme	deep	221:224	arg1	understanding					226:238	a deep understanding	219:238	a deep understanding of the interaction between cells and scaffolds during the process of tissue formation in an artificial environment	219:353	OBJECTIVES The conversion of tissue engineering into a routine clinical tool cannot be achieved without a deep understanding of the interaction between cells and scaffolds during the process of tissue formation in an artificial environment.
30900363	0	32	theme	chitosan-gelatin-polylactide	34:61	arg1	scaffold					63:70	biodegradable chitosan-gelatin-polylactide scaffold	20:70	biodegradable chitosan-gelatin-polylactide scaffold for bioengineering of tracheal epithelium	20:112	Non-woven bilayered biodegradable chitosan-gelatin-polylactide scaffold for bioengineering of tracheal epithelium.
30900363	1	33	theme	formation	316:324	arg1	process					298:304	the process	294:304	the process of tissue formation in an artificial environment	294:353	OBJECTIVES The conversion of tissue engineering into a routine clinical tool cannot be achieved without a deep understanding of the interaction between cells and scaffolds during the process of tissue formation in an artificial environment.
30900363	1	34	from	process	298:304	arg1	environment					343:353	an artificial environment	329:353	an artificial environment	329:353	OBJECTIVES The conversion of tissue engineering into a routine clinical tool cannot be achieved without a deep understanding of the interaction between cells and scaffolds during the process of tissue formation in an artificial environment.
30900363	12	35	theme	pseudostratified	1915:1930	arg1	epithelium					1939:1948	pseudostratified airway epithelium	1915:1948	pseudostratified airway epithelium	1915:1948	For generation of skin equivalent, the lack of diffusion is not so critical as for pseudostratified airway epithelium.
30900363	9	36	theme	differentiation	1296:1310	arg1	analysis					1273:1280	A comparative analysis	1259:1280	A comparative analysis of growth and differentiation for both types of epithelium	1259:1339	A comparative analysis of growth and differentiation for both types of epithelium was performed.
30900363	0	37	theme	biodegradable	20:32	arg1	scaffold					63:70	biodegradable chitosan-gelatin-polylactide scaffold	20:70	biodegradable chitosan-gelatin-polylactide scaffold for bioengineering of tracheal epithelium	20:112	Non-woven bilayered biodegradable chitosan-gelatin-polylactide scaffold for bioengineering of tracheal epithelium.
30900363	11	38	theme	non-woven	1582:1590	arg1	scaffold					1592:1599	non-woven scaffold	1582:1599	non-woven scaffold	1582:1599	The major challenges on the way to obtain the well-differentiated equivalent of respiratory epithelium on non-woven scaffold include the following: the balance between scaffold permeability and thickness, proper combination of synthetic and natural components, and culture conditions sufficient for co-culturing of airway epithelial cells and fibroblasts.
30900363	1	39	theme	tissue	144:149	arg1	engineering					151:161	tissue engineering	144:161	tissue engineering	144:161	OBJECTIVES The conversion of tissue engineering into a routine clinical tool cannot be achieved without a deep understanding of the interaction between cells and scaffolds during the process of tissue formation in an artificial environment.
30900363	10	40	theme	polymer	1455:1461	arg1	composition					1463:1473	polymer composition	1455:1473	polymer composition	1455:1473	CONCLUSIONS The structural parameters of nanofibres should be selected experimentally depending on polymer composition.
30900363	2	41	theme	material	465:472	arg1	conditions					399:408	the cultivation conditions	383:408	the cultivation conditions	383:408	Here, we have investigated the cultivation conditions and structural features of the biodegradable non-woven material in order to obtain a well-differentiated human airway epithelium.
30900363	2	41	theme	material	465:472	arg1	features					425:432	structural features	414:432	structural features	414:432	Here, we have investigated the cultivation conditions and structural features of the biodegradable non-woven material in order to obtain a well-differentiated human airway epithelium.
30900363	2	42	theme	structural	414:423	arg1	features					425:432	structural features	414:432	structural features	414:432	Here, we have investigated the cultivation conditions and structural features of the biodegradable non-woven material in order to obtain a well-differentiated human airway epithelium.
30900363	2	43	theme	non-woven	455:463	arg1	material					465:472	the biodegradable non-woven material	437:472	the biodegradable non-woven material	437:472	Here, we have investigated the cultivation conditions and structural features of the biodegradable non-woven material in order to obtain a well-differentiated human airway epithelium.
30900363	2	44	theme	airway	521:526	arg1	epithelium					528:537	a well-differentiated human airway epithelium	493:537	a well-differentiated human airway epithelium	493:537	Here, we have investigated the cultivation conditions and structural features of the biodegradable non-woven material in order to obtain a well-differentiated human airway epithelium.
30900363	1	45	theme	artificial	332:341	arg1	environment					343:353	an artificial environment	329:353	an artificial environment	329:353	OBJECTIVES The conversion of tissue engineering into a routine clinical tool cannot be achieved without a deep understanding of the interaction between cells and scaffolds during the process of tissue formation in an artificial environment.
30900363	11	46	theme	natural	1717:1723	arg1	components					1725:1734	synthetic and natural components	1703:1734	synthetic and natural components	1703:1734	The major challenges on the way to obtain the well-differentiated equivalent of respiratory epithelium on non-woven scaffold include the following: the balance between scaffold permeability and thickness, proper combination of synthetic and natural components, and culture conditions sufficient for co-culturing of airway epithelial cells and fibroblasts.
30900363	1	47	theme	interaction	247:257	arg1	understanding					226:238	a deep understanding	219:238	a deep understanding of the interaction between cells and scaffolds during the process of tissue formation in an artificial environment	219:353	OBJECTIVES The conversion of tissue engineering into a routine clinical tool cannot be achieved without a deep understanding of the interaction between cells and scaffolds during the process of tissue formation in an artificial environment.
30900363	2	48	theme	human	515:519	arg1	epithelium					528:537	a well-differentiated human airway epithelium	493:537	a well-differentiated human airway epithelium	493:537	Here, we have investigated the cultivation conditions and structural features of the biodegradable non-woven material in order to obtain a well-differentiated human airway epithelium.
30900363	6	49	dep	cell	961:964	arg1	differentiation					984:998	differentiation	984:998	differentiation	984:998	The optimal structural parameters of both layers for cell proliferation and differentiation were determined.
30900363	6	49	dep	cell	961:964	arg1	proliferation					966:978	proliferation	966:978	proliferation	966:978	The optimal structural parameters of both layers for cell proliferation and differentiation were determined.
30900363	5	50	theme	bilayered	846:854	arg1	chitosan-gelatin-poly-l-lactide					811:841	chitosan-gelatin-poly-l-lactide	811:841	chitosan-gelatin-poly-l-lactide	811:841	RESULTS With the use of a copolymer of chitosan-gelatin-poly-l-lactide, a bilayered non-woven scaffold was generated and characterized.
30900363	5	50	theme	bilayered	846:854	arg1	scaffold					866:873	a bilayered non-woven scaffold	844:873	a bilayered non-woven scaffold	844:873	RESULTS With the use of a copolymer of chitosan-gelatin-poly-l-lactide, a bilayered non-woven scaffold was generated and characterized.
30900363	1	51	theme	engineering	151:161	arg1	conversion					130:139	The conversion	126:139	The conversion of tissue engineering into a routine clinical tool	126:190	OBJECTIVES The conversion of tissue engineering into a routine clinical tool cannot be achieved without a deep understanding of the interaction between cells and scaffolds during the process of tissue formation in an artificial environment.
30900363	2	52	theme	well-differentiated	495:513	arg1	epithelium					528:537	a well-differentiated human airway epithelium	493:537	a well-differentiated human airway epithelium	493:537	Here, we have investigated the cultivation conditions and structural features of the biodegradable non-woven material in order to obtain a well-differentiated human airway epithelium.
30900363	8	53	theme	skin	1209:1212	arg1	equivalent					1214:1223	a skin equivalent	1207:1223	a skin equivalent	1207:1223	In addition, keratinocytes formed a skin equivalent when seeded on the same scaffold.
30900363	6	54	theme	layers	950:955	arg1	parameters					931:940	The optimal structural parameters	908:940	The optimal structural parameters of both layers for cell proliferation and differentiation	908:998	The optimal structural parameters of both layers for cell proliferation and differentiation were determined.
30900363	11	55	theme	components	1725:1734	arg1	balance					1628:1634	the balance	1624:1634	the balance between scaffold permeability and thickness	1624:1678	The major challenges on the way to obtain the well-differentiated equivalent of respiratory epithelium on non-woven scaffold include the following: the balance between scaffold permeability and thickness, proper combination of synthetic and natural components, and culture conditions sufficient for co-culturing of airway epithelial cells and fibroblasts.
30900363	11	55	theme	components	1725:1734	arg1	following					1613:1621	the following	1609:1621	the following	1609:1621	The major challenges on the way to obtain the well-differentiated equivalent of respiratory epithelium on non-woven scaffold include the following: the balance between scaffold permeability and thickness, proper combination of synthetic and natural components, and culture conditions sufficient for co-culturing of airway epithelial cells and fibroblasts.
30900363	11	55	theme	components	1725:1734	arg1	combination					1688:1698	proper combination	1681:1698	proper combination of synthetic and natural components	1681:1734	The major challenges on the way to obtain the well-differentiated equivalent of respiratory epithelium on non-woven scaffold include the following: the balance between scaffold permeability and thickness, proper combination of synthetic and natural components, and culture conditions sufficient for co-culturing of airway epithelial cells and fibroblasts.
30900363	11	55	theme	components	1725:1734	arg1	conditions					1749:1758	culture conditions	1741:1758	culture conditions sufficient for co-culturing of airway epithelial cells and fibroblasts	1741:1829	The major challenges on the way to obtain the well-differentiated equivalent of respiratory epithelium on non-woven scaffold include the following: the balance between scaffold permeability and thickness, proper combination of synthetic and natural components, and culture conditions sufficient for co-culturing of airway epithelial cells and fibroblasts.
30900363	11	56	theme	sufficient	1760:1769	arg1	conditions					1749:1758	culture conditions	1741:1758	culture conditions sufficient for co-culturing of airway epithelial cells and fibroblasts	1741:1829	The major challenges on the way to obtain the well-differentiated equivalent of respiratory epithelium on non-woven scaffold include the following: the balance between scaffold permeability and thickness, proper combination of synthetic and natural components, and culture conditions sufficient for co-culturing of airway epithelial cells and fibroblasts.
30900363	1	57	dep	OBJECTIVES	115:124	arg1	achieved					202:209	achieved	202:209	be achieved without a deep understanding of the interaction between cells and scaffolds during the process of tissue formation in an artificial environment	199:353	OBJECTIVES The conversion of tissue engineering into a routine clinical tool cannot be achieved without a deep understanding of the interaction between cells and scaffolds during the process of tissue formation in an artificial environment.
30900363	7	58	theme	airway	1027:1032	arg1	cells					1045:1049	The basal airway epithelial cells	1017:1049	The basal airway epithelial cells	1017:1049	The basal airway epithelial cells differentiated into ciliary and goblet cells and formed pseudostratified epithelial layer on the surface of the scaffold.
30900363	8	59	theme	same	1244:1247	arg1	scaffold					1249:1256	the same scaffold	1240:1256	the same scaffold	1240:1256	In addition, keratinocytes formed a skin equivalent when seeded on the same scaffold.
30900363	11	60	theme	culture	1741:1747	arg1	conditions					1749:1758	culture conditions	1741:1758	culture conditions sufficient for co-culturing of airway epithelial cells and fibroblasts	1741:1829	The major challenges on the way to obtain the well-differentiated equivalent of respiratory epithelium on non-woven scaffold include the following: the balance between scaffold permeability and thickness, proper combination of synthetic and natural components, and culture conditions sufficient for co-culturing of airway epithelial cells and fibroblasts.
30900363	11	61	theme	synthetic	1703:1711	arg1	components					1725:1734	synthetic and natural components	1703:1734	synthetic and natural components	1703:1734	The major challenges on the way to obtain the well-differentiated equivalent of respiratory epithelium on non-woven scaffold include the following: the balance between scaffold permeability and thickness, proper combination of synthetic and natural components, and culture conditions sufficient for co-culturing of airway epithelial cells and fibroblasts.
30900363	9	62	theme	comparative	1261:1271	arg1	analysis					1273:1280	A comparative analysis	1259:1280	A comparative analysis of growth and differentiation for both types of epithelium	1259:1339	A comparative analysis of growth and differentiation for both types of epithelium was performed.
30900363	7	63	theme	epithelial	1034:1043	arg1	cells					1045:1049	The basal airway epithelial cells	1017:1049	The basal airway epithelial cells	1017:1049	The basal airway epithelial cells differentiated into ciliary and goblet cells and formed pseudostratified epithelial layer on the surface of the scaffold.
30900363	7	64	theme	scaffold	1163:1170	arg1	surface					1148:1154	the surface	1144:1154	the surface of the scaffold	1144:1170	The basal airway epithelial cells differentiated into ciliary and goblet cells and formed pseudostratified epithelial layer on the surface of the scaffold.
30900363	11	65	from	equivalent	1542:1551	arg1	scaffold					1592:1599	non-woven scaffold	1582:1599	non-woven scaffold	1582:1599	The major challenges on the way to obtain the well-differentiated equivalent of respiratory epithelium on non-woven scaffold include the following: the balance between scaffold permeability and thickness, proper combination of synthetic and natural components, and culture conditions sufficient for co-culturing of airway epithelial cells and fibroblasts.
30900363	7	66	theme	epithelial	1124:1133	arg1	layer					1135:1139	pseudostratified epithelial layer	1107:1139	pseudostratified epithelial layer	1107:1139	The basal airway epithelial cells differentiated into ciliary and goblet cells and formed pseudostratified epithelial layer on the surface of the scaffold.
30900363	1	67	theme	routine	170:176	arg1	tool					187:190	a routine clinical tool	168:190	a routine clinical tool	168:190	OBJECTIVES The conversion of tissue engineering into a routine clinical tool cannot be achieved without a deep understanding of the interaction between cells and scaffolds during the process of tissue formation in an artificial environment.
30900363	11	68	theme	scaffold	1644:1651	arg1	permeability					1653:1664	scaffold permeability	1644:1664	scaffold permeability	1644:1664	The major challenges on the way to obtain the well-differentiated equivalent of respiratory epithelium on non-woven scaffold include the following: the balance between scaffold permeability and thickness, proper combination of synthetic and natural components, and culture conditions sufficient for co-culturing of airway epithelial cells and fibroblasts.
30900363	0	69	theme	epithelium	103:112	arg1	bioengineering					76:89	bioengineering	76:89	bioengineering of tracheal epithelium	76:112	Non-woven bilayered biodegradable chitosan-gelatin-polylactide scaffold for bioengineering of tracheal epithelium.
30900363	2	70	theme	biodegradable	441:453	arg1	material					465:472	the biodegradable non-woven material	437:472	the biodegradable non-woven material	437:472	Here, we have investigated the cultivation conditions and structural features of the biodegradable non-woven material in order to obtain a well-differentiated human airway epithelium.
30900363	11	71	theme	proper	1681:1686	arg1	combination					1688:1698	proper combination	1681:1698	proper combination of synthetic and natural components	1681:1734	The major challenges on the way to obtain the well-differentiated equivalent of respiratory epithelium on non-woven scaffold include the following: the balance between scaffold permeability and thickness, proper combination of synthetic and natural components, and culture conditions sufficient for co-culturing of airway epithelial cells and fibroblasts.
30900363	1	72	theme	clinical	178:185	arg1	tool					187:190	a routine clinical tool	168:190	a routine clinical tool	168:190	OBJECTIVES The conversion of tissue engineering into a routine clinical tool cannot be achieved without a deep understanding of the interaction between cells and scaffolds during the process of tissue formation in an artificial environment.
30900363	7	73	theme	basal	1021:1025	arg1	cells					1045:1049	The basal airway epithelial cells	1017:1049	The basal airway epithelial cells	1017:1049	The basal airway epithelial cells differentiated into ciliary and goblet cells and formed pseudostratified epithelial layer on the surface of the scaffold.
30900363	4	74	theme	cell	691:694	arg1	assay					710:714	MTT cell proliferation assay	687:714	MTT cell proliferation assay	687:714	The efficiency of the scaffold has been evaluated using MTT cell proliferation assay, histology, immunofluorescence and electron microscopy.
30900363	0	75	theme	tracheal	94:101	arg1	epithelium					103:112	tracheal epithelium	94:112	tracheal epithelium	94:112	Non-woven bilayered biodegradable chitosan-gelatin-polylactide scaffold for bioengineering of tracheal epithelium.
30900363	11	76	theme	major	1480:1484	arg1	challenges					1486:1495	The major challenges	1476:1495	The major challenges on the way to obtain the well-differentiated equivalent of respiratory epithelium on non-woven scaffold	1476:1599	The major challenges on the way to obtain the well-differentiated equivalent of respiratory epithelium on non-woven scaffold include the following: the balance between scaffold permeability and thickness, proper combination of synthetic and natural components, and culture conditions sufficient for co-culturing of airway epithelial cells and fibroblasts.
30900363	9	77	theme	growth	1285:1290	arg1	analysis					1273:1280	A comparative analysis	1259:1280	A comparative analysis of growth and differentiation for both types of epithelium	1259:1339	A comparative analysis of growth and differentiation for both types of epithelium was performed.
30900363	11	78	theme	airway	1791:1796	arg1	cells					1809:1813	airway epithelial cells	1791:1813	airway epithelial cells	1791:1813	The major challenges on the way to obtain the well-differentiated equivalent of respiratory epithelium on non-woven scaffold include the following: the balance between scaffold permeability and thickness, proper combination of synthetic and natural components, and culture conditions sufficient for co-culturing of airway epithelial cells and fibroblasts.
30900363	4	79	theme	MTT	687:689	arg1	assay					710:714	MTT cell proliferation assay	687:714	MTT cell proliferation assay	687:714	The efficiency of the scaffold has been evaluated using MTT cell proliferation assay, histology, immunofluorescence and electron microscopy.
30028308	3	0	theme	HAdV-D37	902:909	arg1	strain					911:916	HAdV-D37 strain	902:916	HAdV-D37 strain	902:916	METHODS Cacicol® was compared to vehicle in the following viral strains: HSV-1 SC16 strain and HSV-1 PSLR, a clinical isolate highly resistant to acyclovir and foscarnet; VZV ATH and VZV FLO, two VZV clinical isolates; and HAdV-D37 strain.
30028308	9	1	theme	HSV-1	1763:1767	arg1	therapy					1744:1750	an adjunct or alternative therapy	1718:1750	therapy	1744:1750	Clinical studies are necessary to determine Cacicol® for an adjunct or alternative therapy of corneal HSV-1 or VZV infection, particularly for the management of antiviral resistant HSV-1.
30028308	2	2	theme	zoster	581:586	arg1	virus					588:592	varicella zoster virus	571:592	varicella zoster virus (VZV)	571:598	Based on the chemical composition, we hypothesized that Cacicol® may compete with natural heparan sulfate (HS) which initiates cell surface attachment of herpes simplex virus type-1 (HSV-1), varicella zoster virus (VZV) and human adenovirus (HAdV), three viruses associated with corneal infections.
30028308	2	2	theme	zoster	581:586	arg1	VZV					595:597	VZV	595:597	VZV	595:597	Based on the chemical composition, we hypothesized that Cacicol® may compete with natural heparan sulfate (HS) which initiates cell surface attachment of herpes simplex virus type-1 (HSV-1), varicella zoster virus (VZV) and human adenovirus (HAdV), three viruses associated with corneal infections.
30028308	2	3	theme	human	604:608	arg1	HAdV					622:625	HAdV	622:625	HAdV	622:625	Based on the chemical composition, we hypothesized that Cacicol® may compete with natural heparan sulfate (HS) which initiates cell surface attachment of herpes simplex virus type-1 (HSV-1), varicella zoster virus (VZV) and human adenovirus (HAdV), three viruses associated with corneal infections.
30028308	2	3	theme	human	604:608	arg1	adenovirus					610:619	human adenovirus	604:619	human adenovirus (HAdV)	604:626	Based on the chemical composition, we hypothesized that Cacicol® may compete with natural heparan sulfate (HS) which initiates cell surface attachment of herpes simplex virus type-1 (HSV-1), varicella zoster virus (VZV) and human adenovirus (HAdV), three viruses associated with corneal infections.
30028308	4	4	theme	plaque	1035:1040	arg1	assays					1052:1057	plaque reduction assays	1035:1057	plaque reduction assays on Vero cells for HSV-1 and MeWo cells for VZV	1035:1104	Viruses in Cacicol® or vehicle were added to cells for 1 h during adsorption then viral replication was assessed by plaque reduction assays on Vero cells for HSV-1 and MeWo cells for VZV and by immunostaining assay on Hep-2 cells for HAdV-D37.
30028308	5	5	theme	VZV	1280:1282	arg1	FLO					1284:1286	VZV FLO	1280:1286	VZV FLO	1280:1286	RESULTS The vehicle had no effect, dose-dependent effects were demonstrated when HSV-1 SC16, HSV-1 PSLR, VZV ATH and VZV FLO were inoculated in the presence of Cacicol®, inhibiting viral replication by 98.4%, 98.9%, 90.1% and 89.0%, respectively.
30028308	1	6	theme	BACKGROUND	164:173	arg1	Cacicol®					175:182	BACKGROUND Cacicol®	164:182	BACKGROUND Cacicol®	164:182	BACKGROUND Cacicol®, a topical eye biopolymer containing a poly-carboxymethylglucose sulfate solution that is a regenerating matrix therapy agent, intended for wound healing of persistent corneal epithelial defects.
30028308	1	6	theme	BACKGROUND	164:173	arg1	biopolymer					199:208	a topical eye biopolymer	185:208	a topical eye biopolymer	185:208	BACKGROUND Cacicol®, a topical eye biopolymer containing a poly-carboxymethylglucose sulfate solution that is a regenerating matrix therapy agent, intended for wound healing of persistent corneal epithelial defects.
30028308	5	7	dep	RESULTS	1163:1169	arg1	had					1183:1185	had	1183:1185	had no effect	1183:1195	RESULTS The vehicle had no effect, dose-dependent effects were demonstrated when HSV-1 SC16, HSV-1 PSLR, VZV ATH and VZV FLO were inoculated in the presence of Cacicol®, inhibiting viral replication by 98.4%, 98.9%, 90.1% and 89.0%, respectively.
30028308	0	8	theme	simplex	105:111	arg1	type-1					119:124	herpes simplex virus type-1 and varicella zoster virus	98:151	type-1	119:124	Antiviral effects of Cacicol®, a heparan sulfate biomimetic for corneal regeneration therapy, for herpes simplex virus type-1 and varicella zoster virus infection.
30028308	1	9	theme	topical	187:193	arg1	Cacicol®					175:182	BACKGROUND Cacicol®	164:182	BACKGROUND Cacicol®	164:182	BACKGROUND Cacicol®, a topical eye biopolymer containing a poly-carboxymethylglucose sulfate solution that is a regenerating matrix therapy agent, intended for wound healing of persistent corneal epithelial defects.
30028308	1	9	theme	topical	187:193	arg1	biopolymer					199:208	a topical eye biopolymer	185:208	a topical eye biopolymer	185:208	BACKGROUND Cacicol®, a topical eye biopolymer containing a poly-carboxymethylglucose sulfate solution that is a regenerating matrix therapy agent, intended for wound healing of persistent corneal epithelial defects.
30028308	2	10	theme	simplex	541:547	arg1	virus					549:553	herpes simplex virus type-1 (HSV-1)	534:568	herpes simplex virus type-1 (HSV-1)	534:568	Based on the chemical composition, we hypothesized that Cacicol® may compete with natural heparan sulfate (HS) which initiates cell surface attachment of herpes simplex virus type-1 (HSV-1), varicella zoster virus (VZV) and human adenovirus (HAdV), three viruses associated with corneal infections.
30028308	9	11	theme	corneal	1755:1761	arg1	HSV-1					1763:1767	corneal HSV-1 or VZV infection	1755:1784	HSV-1	1763:1767	Clinical studies are necessary to determine Cacicol® for an adjunct or alternative therapy of corneal HSV-1 or VZV infection, particularly for the management of antiviral resistant HSV-1.
30028308	9	12	theme	resistant	1832:1840	arg1	HSV-1					1842:1846	antiviral resistant HSV-1	1822:1846	antiviral resistant HSV-1	1822:1846	Clinical studies are necessary to determine Cacicol® for an adjunct or alternative therapy of corneal HSV-1 or VZV infection, particularly for the management of antiviral resistant HSV-1.
30028308	0	13	theme	type-1	119:124	arg1	infection					153:161	herpes simplex virus type-1 and varicella zoster virus infection	98:161	herpes simplex virus type-1 and varicella zoster virus infection	98:161	Antiviral effects of Cacicol®, a heparan sulfate biomimetic for corneal regeneration therapy, for herpes simplex virus type-1 and varicella zoster virus infection.
30028308	5	14	contain	had	1183:1185	arg1	vehicle					1175:1181	The vehicle	1171:1181	The vehicle	1171:1181	RESULTS The vehicle had no effect, dose-dependent effects were demonstrated when HSV-1 SC16, HSV-1 PSLR, VZV ATH and VZV FLO were inoculated in the presence of Cacicol®, inhibiting viral replication by 98.4%, 98.9%, 90.1% and 89.0%, respectively.
30028308	5	14	contain	had	1183:1185	arg2	effect					1190:1195	no effect	1187:1195	no effect	1187:1195	RESULTS The vehicle had no effect, dose-dependent effects were demonstrated when HSV-1 SC16, HSV-1 PSLR, VZV ATH and VZV FLO were inoculated in the presence of Cacicol®, inhibiting viral replication by 98.4%, 98.9%, 90.1% and 89.0%, respectively.
30028308	6	15	theme	antiviral	1426:1434	arg1	effect					1436:1441	no antiviral effect	1423:1441	no antiviral effect	1423:1441	Cacicol® had no antiviral effect against HAdV-D37.
30028308	3	16	theme	following	727:735	arg1	strain					763:768	HSV-1 SC16 strain	752:768	HSV-1 SC16 strain	752:768	METHODS Cacicol® was compared to vehicle in the following viral strains: HSV-1 SC16 strain and HSV-1 PSLR, a clinical isolate highly resistant to acyclovir and foscarnet; VZV ATH and VZV FLO, two VZV clinical isolates; and HAdV-D37 strain.
30028308	3	16	theme	following	727:735	arg1	PSLR					780:783	HSV-1 PSLR	774:783	HSV-1 PSLR	774:783	METHODS Cacicol® was compared to vehicle in the following viral strains: HSV-1 SC16 strain and HSV-1 PSLR, a clinical isolate highly resistant to acyclovir and foscarnet; VZV ATH and VZV FLO, two VZV clinical isolates; and HAdV-D37 strain.
30028308	3	16	theme	following	727:735	arg1	strains					743:749	the following viral strains	723:749	the following viral strains: HSV-1 SC16 strain and HSV-1 PSLR	723:783	METHODS Cacicol® was compared to vehicle in the following viral strains: HSV-1 SC16 strain and HSV-1 PSLR, a clinical isolate highly resistant to acyclovir and foscarnet; VZV ATH and VZV FLO, two VZV clinical isolates; and HAdV-D37 strain.
30028308	0	17	theme	varicella	130:138	arg1	virus					147:151	herpes simplex virus type-1 and varicella zoster virus	98:151	virus	147:151	Antiviral effects of Cacicol®, a heparan sulfate biomimetic for corneal regeneration therapy, for herpes simplex virus type-1 and varicella zoster virus infection.
30028308	8	18	theme	cell	1649:1652	arg1	entry					1654:1658	cell entry	1649:1658	cell entry	1649:1658	The lack of effect on HAdV is probably because it is less dependent on HS interactions for cell entry.
30028308	9	19	theme	infection	1776:1784	arg1	therapy					1744:1750	an adjunct or alternative therapy	1718:1750	therapy	1744:1750	Clinical studies are necessary to determine Cacicol® for an adjunct or alternative therapy of corneal HSV-1 or VZV infection, particularly for the management of antiviral resistant HSV-1.
30028308	0	20	theme	virus	147:151	arg1	infection					153:161	herpes simplex virus type-1 and varicella zoster virus infection	98:161	herpes simplex virus type-1 and varicella zoster virus infection	98:161	Antiviral effects of Cacicol®, a heparan sulfate biomimetic for corneal regeneration therapy, for herpes simplex virus type-1 and varicella zoster virus infection.
30028308	3	21	theme	clinical	788:795	arg1	isolate					797:803	a clinical isolate	786:803	a clinical isolate highly resistant to acyclovir and foscarnet	786:847	METHODS Cacicol® was compared to vehicle in the following viral strains: HSV-1 SC16 strain and HSV-1 PSLR, a clinical isolate highly resistant to acyclovir and foscarnet; VZV ATH and VZV FLO, two VZV clinical isolates; and HAdV-D37 strain.
30028308	4	22	theme	Vero	1062:1065	arg1	cells					1067:1071	Vero cells	1062:1071	Vero cells for HSV-1	1062:1081	Viruses in Cacicol® or vehicle were added to cells for 1 h during adsorption then viral replication was assessed by plaque reduction assays on Vero cells for HSV-1 and MeWo cells for VZV and by immunostaining assay on Hep-2 cells for HAdV-D37.
30028308	3	23	theme	HSV-1	752:756	arg1	SC16					758:761	HSV-1 SC16	752:761	HSV-1 SC16 strain	752:768	METHODS Cacicol® was compared to vehicle in the following viral strains: HSV-1 SC16 strain and HSV-1 PSLR, a clinical isolate highly resistant to acyclovir and foscarnet; VZV ATH and VZV FLO, two VZV clinical isolates; and HAdV-D37 strain.
30028308	0	24	theme	biomimetic	49:58	arg1	Cacicol®					21:28	Cacicol®	21:28	Cacicol®	21:28	Antiviral effects of Cacicol®, a heparan sulfate biomimetic for corneal regeneration therapy, for herpes simplex virus type-1 and varicella zoster virus infection.
30028308	0	24	theme	biomimetic	49:58	arg1	sulfate					41:47	a heparan sulfate	31:47	a heparan sulfate biomimetic for corneal regeneration therapy	31:91	Antiviral effects of Cacicol®, a heparan sulfate biomimetic for corneal regeneration therapy, for herpes simplex virus type-1 and varicella zoster virus infection.
30028308	0	25	theme	corneal	64:70	arg1	therapy					85:91	corneal regeneration therapy	64:91	corneal regeneration therapy	64:91	Antiviral effects of Cacicol®, a heparan sulfate biomimetic for corneal regeneration therapy, for herpes simplex virus type-1 and varicella zoster virus infection.
30028308	1	26	theme	persistent	341:350	arg1	defects					371:377	persistent corneal epithelial defects	341:377	persistent corneal epithelial defects	341:377	BACKGROUND Cacicol®, a topical eye biopolymer containing a poly-carboxymethylglucose sulfate solution that is a regenerating matrix therapy agent, intended for wound healing of persistent corneal epithelial defects.
30028308	3	27	theme	HSV-1	774:778	arg1	PSLR					780:783	HSV-1 PSLR	774:783	HSV-1 PSLR	774:783	METHODS Cacicol® was compared to vehicle in the following viral strains: HSV-1 SC16 strain and HSV-1 PSLR, a clinical isolate highly resistant to acyclovir and foscarnet; VZV ATH and VZV FLO, two VZV clinical isolates; and HAdV-D37 strain.
30028308	3	27	theme	HSV-1	774:778	arg1	strains					743:749	the following viral strains	723:749	the following viral strains: HSV-1 SC16 strain and HSV-1 PSLR	723:783	METHODS Cacicol® was compared to vehicle in the following viral strains: HSV-1 SC16 strain and HSV-1 PSLR, a clinical isolate highly resistant to acyclovir and foscarnet; VZV ATH and VZV FLO, two VZV clinical isolates; and HAdV-D37 strain.
30028308	1	28	theme	epithelial	360:369	arg1	defects					371:377	persistent corneal epithelial defects	341:377	persistent corneal epithelial defects	341:377	BACKGROUND Cacicol®, a topical eye biopolymer containing a poly-carboxymethylglucose sulfate solution that is a regenerating matrix therapy agent, intended for wound healing of persistent corneal epithelial defects.
30028308	8	29	theme	HS	1629:1630	arg1	interactions					1632:1643	HS interactions	1629:1643	HS interactions for cell entry	1629:1658	The lack of effect on HAdV is probably because it is less dependent on HS interactions for cell entry.
30028308	3	30	theme	VZV	862:864	arg1	isolates					888:895	VZV ATH and VZV FLO, two VZV clinical isolates	850:895	isolates	888:895	METHODS Cacicol® was compared to vehicle in the following viral strains: HSV-1 SC16 strain and HSV-1 PSLR, a clinical isolate highly resistant to acyclovir and foscarnet; VZV ATH and VZV FLO, two VZV clinical isolates; and HAdV-D37 strain.
30028308	2	31	theme	surface	512:518	arg1	attachment					520:529	cell surface attachment	507:529	cell surface attachment of herpes simplex virus type-1 (HSV-1), varicella zoster virus (VZV) and human adenovirus (HAdV), three viruses associated with corneal infections	507:676	Based on the chemical composition, we hypothesized that Cacicol® may compete with natural heparan sulfate (HS) which initiates cell surface attachment of herpes simplex virus type-1 (HSV-1), varicella zoster virus (VZV) and human adenovirus (HAdV), three viruses associated with corneal infections.
30028308	5	32	theme	Cacicol®	1323:1330	arg1	presence					1311:1318	the presence	1307:1318	the presence of Cacicol®	1307:1330	RESULTS The vehicle had no effect, dose-dependent effects were demonstrated when HSV-1 SC16, HSV-1 PSLR, VZV ATH and VZV FLO were inoculated in the presence of Cacicol®, inhibiting viral replication by 98.4%, 98.9%, 90.1% and 89.0%, respectively.
30028308	2	33	theme	adenovirus	610:619	arg1	attachment					520:529	cell surface attachment	507:529	cell surface attachment of herpes simplex virus type-1 (HSV-1), varicella zoster virus (VZV) and human adenovirus (HAdV), three viruses associated with corneal infections	507:676	Based on the chemical composition, we hypothesized that Cacicol® may compete with natural heparan sulfate (HS) which initiates cell surface attachment of herpes simplex virus type-1 (HSV-1), varicella zoster virus (VZV) and human adenovirus (HAdV), three viruses associated with corneal infections.
30028308	3	34	dep	strains	743:749	arg1	strain					763:768	HSV-1 SC16 strain	752:768	HSV-1 SC16 strain	752:768	METHODS Cacicol® was compared to vehicle in the following viral strains: HSV-1 SC16 strain and HSV-1 PSLR, a clinical isolate highly resistant to acyclovir and foscarnet; VZV ATH and VZV FLO, two VZV clinical isolates; and HAdV-D37 strain.
30028308	3	34	dep	strains	743:749	arg1	PSLR					780:783	HSV-1 PSLR	774:783	HSV-1 PSLR	774:783	METHODS Cacicol® was compared to vehicle in the following viral strains: HSV-1 SC16 strain and HSV-1 PSLR, a clinical isolate highly resistant to acyclovir and foscarnet; VZV ATH and VZV FLO, two VZV clinical isolates; and HAdV-D37 strain.
30028308	3	34	dep	strains	743:749	arg1	strains					743:749	the following viral strains	723:749	the following viral strains: HSV-1 SC16 strain and HSV-1 PSLR	723:783	METHODS Cacicol® was compared to vehicle in the following viral strains: HSV-1 SC16 strain and HSV-1 PSLR, a clinical isolate highly resistant to acyclovir and foscarnet; VZV ATH and VZV FLO, two VZV clinical isolates; and HAdV-D37 strain.
30028308	4	35	from	assay	1128:1132	arg1	cells					1143:1147	Hep-2 cells	1137:1147	Hep-2 cells	1137:1147	Viruses in Cacicol® or vehicle were added to cells for 1 h during adsorption then viral replication was assessed by plaque reduction assays on Vero cells for HSV-1 and MeWo cells for VZV and by immunostaining assay on Hep-2 cells for HAdV-D37.
30028308	2	36	theme	viruses	635:641	arg1	attachment					520:529	cell surface attachment	507:529	cell surface attachment of herpes simplex virus type-1 (HSV-1), varicella zoster virus (VZV) and human adenovirus (HAdV), three viruses associated with corneal infections	507:676	Based on the chemical composition, we hypothesized that Cacicol® may compete with natural heparan sulfate (HS) which initiates cell surface attachment of herpes simplex virus type-1 (HSV-1), varicella zoster virus (VZV) and human adenovirus (HAdV), three viruses associated with corneal infections.
30028308	5	37	theme	HSV-1	1244:1248	arg1	SC16					1250:1253	HSV-1 SC16	1244:1253	HSV-1 SC16	1244:1253	RESULTS The vehicle had no effect, dose-dependent effects were demonstrated when HSV-1 SC16, HSV-1 PSLR, VZV ATH and VZV FLO were inoculated in the presence of Cacicol®, inhibiting viral replication by 98.4%, 98.9%, 90.1% and 89.0%, respectively.
30028308	0	38	theme	Antiviral	0:8	arg1	effects					10:16	Antiviral effects	0:16	Antiviral effects of Cacicol®, a heparan sulfate biomimetic for corneal regeneration therapy, for herpes simplex virus type-1 and varicella zoster virus infection.	0:162	Antiviral effects of Cacicol®, a heparan sulfate biomimetic for corneal regeneration therapy, for herpes simplex virus type-1 and varicella zoster virus infection.
30028308	1	39	theme	regenerating	276:287	arg1	agent					304:308	a regenerating matrix therapy agent	274:308	a regenerating matrix therapy agent	274:308	BACKGROUND Cacicol®, a topical eye biopolymer containing a poly-carboxymethylglucose sulfate solution that is a regenerating matrix therapy agent, intended for wound healing of persistent corneal epithelial defects.
30028308	1	39	theme	regenerating	276:287	arg1	solution					257:264	a poly-carboxymethylglucose sulfate solution	221:264	a poly-carboxymethylglucose sulfate solution that is a regenerating matrix therapy agent, intended for wound healing of persistent corneal epithelial defects	221:377	BACKGROUND Cacicol®, a topical eye biopolymer containing a poly-carboxymethylglucose sulfate solution that is a regenerating matrix therapy agent, intended for wound healing of persistent corneal epithelial defects.
30028308	1	40	theme	therapy	296:302	arg1	agent					304:308	a regenerating matrix therapy agent	274:308	a regenerating matrix therapy agent	274:308	BACKGROUND Cacicol®, a topical eye biopolymer containing a poly-carboxymethylglucose sulfate solution that is a regenerating matrix therapy agent, intended for wound healing of persistent corneal epithelial defects.
30028308	1	40	theme	therapy	296:302	arg1	solution					257:264	a poly-carboxymethylglucose sulfate solution	221:264	a poly-carboxymethylglucose sulfate solution that is a regenerating matrix therapy agent, intended for wound healing of persistent corneal epithelial defects	221:377	BACKGROUND Cacicol®, a topical eye biopolymer containing a poly-carboxymethylglucose sulfate solution that is a regenerating matrix therapy agent, intended for wound healing of persistent corneal epithelial defects.
30028308	7	41	contain	has	1482:1484	arg1	Cacicol®					1473:1480	CONCLUSIONS Cacicol®	1461:1480	CONCLUSIONS Cacicol®	1461:1480	CONCLUSIONS Cacicol® has a significant antiviral activity on HSV-1 and VZV, but not on HAdV-D37.
30028308	7	41	contain	has	1482:1484	arg2	activity					1510:1517	a significant antiviral activity	1486:1517	a significant antiviral activity	1486:1517	CONCLUSIONS Cacicol® has a significant antiviral activity on HSV-1 and VZV, but not on HAdV-D37.
30028308	7	42	theme	significant	1488:1498	arg1	activity					1510:1517	a significant antiviral activity	1486:1517	a significant antiviral activity	1486:1517	CONCLUSIONS Cacicol® has a significant antiviral activity on HSV-1 and VZV, but not on HAdV-D37.
30028308	2	43	theme	heparan	470:476	arg1	HS					487:488	HS	487:488	HS	487:488	Based on the chemical composition, we hypothesized that Cacicol® may compete with natural heparan sulfate (HS) which initiates cell surface attachment of herpes simplex virus type-1 (HSV-1), varicella zoster virus (VZV) and human adenovirus (HAdV), three viruses associated with corneal infections.
30028308	2	43	theme	heparan	470:476	arg1	sulfate					478:484	natural heparan sulfate	462:484	natural heparan sulfate (HS) which initiates cell surface attachment of herpes simplex virus type-1 (HSV-1), varicella zoster virus (VZV) and human adenovirus (HAdV), three viruses associated with corneal infections	462:676	Based on the chemical composition, we hypothesized that Cacicol® may compete with natural heparan sulfate (HS) which initiates cell surface attachment of herpes simplex virus type-1 (HSV-1), varicella zoster virus (VZV) and human adenovirus (HAdV), three viruses associated with corneal infections.
30028308	5	44	theme	VZV	1268:1270	arg1	ATH					1272:1274	VZV ATH	1268:1274	VZV ATH	1268:1274	RESULTS The vehicle had no effect, dose-dependent effects were demonstrated when HSV-1 SC16, HSV-1 PSLR, VZV ATH and VZV FLO were inoculated in the presence of Cacicol®, inhibiting viral replication by 98.4%, 98.9%, 90.1% and 89.0%, respectively.
30028308	0	45	theme	heparan	33:39	arg1	Cacicol®					21:28	Cacicol®	21:28	Cacicol®	21:28	Antiviral effects of Cacicol®, a heparan sulfate biomimetic for corneal regeneration therapy, for herpes simplex virus type-1 and varicella zoster virus infection.
30028308	0	45	theme	heparan	33:39	arg1	sulfate					41:47	a heparan sulfate	31:47	a heparan sulfate biomimetic for corneal regeneration therapy	31:91	Antiviral effects of Cacicol®, a heparan sulfate biomimetic for corneal regeneration therapy, for herpes simplex virus type-1 and varicella zoster virus infection.
30028308	3	46	theme	VZV	875:877	arg1	isolates					888:895	VZV ATH and VZV FLO, two VZV clinical isolates	850:895	isolates	888:895	METHODS Cacicol® was compared to vehicle in the following viral strains: HSV-1 SC16 strain and HSV-1 PSLR, a clinical isolate highly resistant to acyclovir and foscarnet; VZV ATH and VZV FLO, two VZV clinical isolates; and HAdV-D37 strain.
30028308	7	47	theme	antiviral	1500:1508	arg1	activity					1510:1517	a significant antiviral activity	1486:1517	a significant antiviral activity	1486:1517	CONCLUSIONS Cacicol® has a significant antiviral activity on HSV-1 and VZV, but not on HAdV-D37.
30028308	4	48	theme	viral	1001:1005	arg1	replication					1007:1017	then viral replication	996:1017	then viral replication	996:1017	Viruses in Cacicol® or vehicle were added to cells for 1 h during adsorption then viral replication was assessed by plaque reduction assays on Vero cells for HSV-1 and MeWo cells for VZV and by immunostaining assay on Hep-2 cells for HAdV-D37.
30028308	4	49	from	assays	1052:1057	arg1	cells					1092:1096	MeWo cells	1087:1096	MeWo cells	1087:1096	Viruses in Cacicol® or vehicle were added to cells for 1 h during adsorption then viral replication was assessed by plaque reduction assays on Vero cells for HSV-1 and MeWo cells for VZV and by immunostaining assay on Hep-2 cells for HAdV-D37.
30028308	4	49	from	assays	1052:1057	arg1	cells					1067:1071	Vero cells	1062:1071	Vero cells for HSV-1	1062:1081	Viruses in Cacicol® or vehicle were added to cells for 1 h during adsorption then viral replication was assessed by plaque reduction assays on Vero cells for HSV-1 and MeWo cells for VZV and by immunostaining assay on Hep-2 cells for HAdV-D37.
30028308	1	50	theme	poly-carboxymethylglucose	223:247	arg1	agent					304:308	a regenerating matrix therapy agent	274:308	a regenerating matrix therapy agent	274:308	BACKGROUND Cacicol®, a topical eye biopolymer containing a poly-carboxymethylglucose sulfate solution that is a regenerating matrix therapy agent, intended for wound healing of persistent corneal epithelial defects.
30028308	1	50	theme	poly-carboxymethylglucose	223:247	arg1	solution					257:264	a poly-carboxymethylglucose sulfate solution	221:264	a poly-carboxymethylglucose sulfate solution that is a regenerating matrix therapy agent, intended for wound healing of persistent corneal epithelial defects	221:377	BACKGROUND Cacicol®, a topical eye biopolymer containing a poly-carboxymethylglucose sulfate solution that is a regenerating matrix therapy agent, intended for wound healing of persistent corneal epithelial defects.
30028308	2	51	theme	virus	588:592	arg1	attachment					520:529	cell surface attachment	507:529	cell surface attachment of herpes simplex virus type-1 (HSV-1), varicella zoster virus (VZV) and human adenovirus (HAdV), three viruses associated with corneal infections	507:676	Based on the chemical composition, we hypothesized that Cacicol® may compete with natural heparan sulfate (HS) which initiates cell surface attachment of herpes simplex virus type-1 (HSV-1), varicella zoster virus (VZV) and human adenovirus (HAdV), three viruses associated with corneal infections.
30028308	2	52	theme	varicella	571:579	arg1	virus					588:592	varicella zoster virus	571:592	varicella zoster virus (VZV)	571:598	Based on the chemical composition, we hypothesized that Cacicol® may compete with natural heparan sulfate (HS) which initiates cell surface attachment of herpes simplex virus type-1 (HSV-1), varicella zoster virus (VZV) and human adenovirus (HAdV), three viruses associated with corneal infections.
30028308	2	52	theme	varicella	571:579	arg1	VZV					595:597	VZV	595:597	VZV	595:597	Based on the chemical composition, we hypothesized that Cacicol® may compete with natural heparan sulfate (HS) which initiates cell surface attachment of herpes simplex virus type-1 (HSV-1), varicella zoster virus (VZV) and human adenovirus (HAdV), three viruses associated with corneal infections.
30028308	4	53	theme	reduction	1042:1050	arg1	assays					1052:1057	plaque reduction assays	1035:1057	plaque reduction assays on Vero cells for HSV-1 and MeWo cells for VZV	1035:1104	Viruses in Cacicol® or vehicle were added to cells for 1 h during adsorption then viral replication was assessed by plaque reduction assays on Vero cells for HSV-1 and MeWo cells for VZV and by immunostaining assay on Hep-2 cells for HAdV-D37.
30028308	2	54	dep	virus	549:553	arg1	HSV-1					563:567	HSV-1	563:567	HSV-1	563:567	Based on the chemical composition, we hypothesized that Cacicol® may compete with natural heparan sulfate (HS) which initiates cell surface attachment of herpes simplex virus type-1 (HSV-1), varicella zoster virus (VZV) and human adenovirus (HAdV), three viruses associated with corneal infections.
30028308	2	54	dep	virus	549:553	arg1	type-1					555:560	type-1	555:560	herpes simplex virus type-1 (HSV-1)	534:568	Based on the chemical composition, we hypothesized that Cacicol® may compete with natural heparan sulfate (HS) which initiates cell surface attachment of herpes simplex virus type-1 (HSV-1), varicella zoster virus (VZV) and human adenovirus (HAdV), three viruses associated with corneal infections.
30028308	9	55	theme	adjunct	1721:1727	arg1	therapy					1744:1750	an adjunct or alternative therapy	1718:1750	therapy	1744:1750	Clinical studies are necessary to determine Cacicol® for an adjunct or alternative therapy of corneal HSV-1 or VZV infection, particularly for the management of antiviral resistant HSV-1.
30028308	6	56	contain	had	1419:1421	arg2	effect					1436:1441	no antiviral effect	1423:1441	no antiviral effect	1423:1441	Cacicol® had no antiviral effect against HAdV-D37.
30028308	6	56	contain	had	1419:1421	arg1	Cacicol®					1410:1417	Cacicol®	1410:1417	Cacicol®	1410:1417	Cacicol® had no antiviral effect against HAdV-D37.
30028308	9	57	theme	VZV	1772:1774	arg1	infection					1776:1784	corneal HSV-1 or VZV infection	1755:1784	infection	1776:1784	Clinical studies are necessary to determine Cacicol® for an adjunct or alternative therapy of corneal HSV-1 or VZV infection, particularly for the management of antiviral resistant HSV-1.
30028308	9	58	theme	alternative	1732:1742	arg1	therapy					1744:1750	an adjunct or alternative therapy	1718:1750	therapy	1744:1750	Clinical studies are necessary to determine Cacicol® for an adjunct or alternative therapy of corneal HSV-1 or VZV infection, particularly for the management of antiviral resistant HSV-1.
30028308	0	59	theme	virus	113:117	arg1	type-1					119:124	herpes simplex virus type-1 and varicella zoster virus	98:151	type-1	119:124	Antiviral effects of Cacicol®, a heparan sulfate biomimetic for corneal regeneration therapy, for herpes simplex virus type-1 and varicella zoster virus infection.
30028308	7	60	theme	CONCLUSIONS	1461:1471	arg1	Cacicol®					1473:1480	CONCLUSIONS Cacicol®	1461:1480	CONCLUSIONS Cacicol®	1461:1480	CONCLUSIONS Cacicol® has a significant antiviral activity on HSV-1 and VZV, but not on HAdV-D37.
30028308	2	61	theme	virus	549:553	arg1	attachment					520:529	cell surface attachment	507:529	cell surface attachment of herpes simplex virus type-1 (HSV-1), varicella zoster virus (VZV) and human adenovirus (HAdV), three viruses associated with corneal infections	507:676	Based on the chemical composition, we hypothesized that Cacicol® may compete with natural heparan sulfate (HS) which initiates cell surface attachment of herpes simplex virus type-1 (HSV-1), varicella zoster virus (VZV) and human adenovirus (HAdV), three viruses associated with corneal infections.
30028308	1	62	theme	eye	195:197	arg1	Cacicol®					175:182	BACKGROUND Cacicol®	164:182	BACKGROUND Cacicol®	164:182	BACKGROUND Cacicol®, a topical eye biopolymer containing a poly-carboxymethylglucose sulfate solution that is a regenerating matrix therapy agent, intended for wound healing of persistent corneal epithelial defects.
30028308	1	62	theme	eye	195:197	arg1	biopolymer					199:208	a topical eye biopolymer	185:208	a topical eye biopolymer	185:208	BACKGROUND Cacicol®, a topical eye biopolymer containing a poly-carboxymethylglucose sulfate solution that is a regenerating matrix therapy agent, intended for wound healing of persistent corneal epithelial defects.
30028308	2	63	theme	herpes	534:539	arg1	virus					549:553	herpes simplex virus type-1 (HSV-1)	534:568	herpes simplex virus type-1 (HSV-1)	534:568	Based on the chemical composition, we hypothesized that Cacicol® may compete with natural heparan sulfate (HS) which initiates cell surface attachment of herpes simplex virus type-1 (HSV-1), varicella zoster virus (VZV) and human adenovirus (HAdV), three viruses associated with corneal infections.
30028308	4	64	theme	MeWo	1087:1090	arg1	cells					1092:1096	MeWo cells	1087:1096	MeWo cells	1087:1096	Viruses in Cacicol® or vehicle were added to cells for 1 h during adsorption then viral replication was assessed by plaque reduction assays on Vero cells for HSV-1 and MeWo cells for VZV and by immunostaining assay on Hep-2 cells for HAdV-D37.
30028308	1	65	theme	wound	324:328	arg1	healing					330:336	wound healing	324:336	wound healing of persistent corneal epithelial defects	324:377	BACKGROUND Cacicol®, a topical eye biopolymer containing a poly-carboxymethylglucose sulfate solution that is a regenerating matrix therapy agent, intended for wound healing of persistent corneal epithelial defects.
30028308	0	66	theme	zoster	140:145	arg1	virus					147:151	herpes simplex virus type-1 and varicella zoster virus	98:151	virus	147:151	Antiviral effects of Cacicol®, a heparan sulfate biomimetic for corneal regeneration therapy, for herpes simplex virus type-1 and varicella zoster virus infection.
30028308	8	67	theme	effect	1570:1575	arg1	lack					1562:1565	The lack	1558:1565	The lack of effect on HAdV	1558:1583	The lack of effect on HAdV is probably because it is less dependent on HS interactions for cell entry.
30028308	2	68	theme	corneal	659:665	arg1	infections					667:676	corneal infections	659:676	corneal infections	659:676	Based on the chemical composition, we hypothesized that Cacicol® may compete with natural heparan sulfate (HS) which initiates cell surface attachment of herpes simplex virus type-1 (HSV-1), varicella zoster virus (VZV) and human adenovirus (HAdV), three viruses associated with corneal infections.
30028308	3	69	theme	viral	737:741	arg1	strain					763:768	HSV-1 SC16 strain	752:768	HSV-1 SC16 strain	752:768	METHODS Cacicol® was compared to vehicle in the following viral strains: HSV-1 SC16 strain and HSV-1 PSLR, a clinical isolate highly resistant to acyclovir and foscarnet; VZV ATH and VZV FLO, two VZV clinical isolates; and HAdV-D37 strain.
30028308	3	69	theme	viral	737:741	arg1	PSLR					780:783	HSV-1 PSLR	774:783	HSV-1 PSLR	774:783	METHODS Cacicol® was compared to vehicle in the following viral strains: HSV-1 SC16 strain and HSV-1 PSLR, a clinical isolate highly resistant to acyclovir and foscarnet; VZV ATH and VZV FLO, two VZV clinical isolates; and HAdV-D37 strain.
30028308	3	69	theme	viral	737:741	arg1	strains					743:749	the following viral strains	723:749	the following viral strains: HSV-1 SC16 strain and HSV-1 PSLR	723:783	METHODS Cacicol® was compared to vehicle in the following viral strains: HSV-1 SC16 strain and HSV-1 PSLR, a clinical isolate highly resistant to acyclovir and foscarnet; VZV ATH and VZV FLO, two VZV clinical isolates; and HAdV-D37 strain.
30028308	3	70	theme	resistant	812:820	arg1	isolate					797:803	a clinical isolate	786:803	a clinical isolate highly resistant to acyclovir and foscarnet	786:847	METHODS Cacicol® was compared to vehicle in the following viral strains: HSV-1 SC16 strain and HSV-1 PSLR, a clinical isolate highly resistant to acyclovir and foscarnet; VZV ATH and VZV FLO, two VZV clinical isolates; and HAdV-D37 strain.
30028308	3	71	theme	SC16	758:761	arg1	strain					763:768	HSV-1 SC16 strain	752:768	HSV-1 SC16 strain	752:768	METHODS Cacicol® was compared to vehicle in the following viral strains: HSV-1 SC16 strain and HSV-1 PSLR, a clinical isolate highly resistant to acyclovir and foscarnet; VZV ATH and VZV FLO, two VZV clinical isolates; and HAdV-D37 strain.
30028308	3	71	theme	SC16	758:761	arg1	strains					743:749	the following viral strains	723:749	the following viral strains: HSV-1 SC16 strain and HSV-1 PSLR	723:783	METHODS Cacicol® was compared to vehicle in the following viral strains: HSV-1 SC16 strain and HSV-1 PSLR, a clinical isolate highly resistant to acyclovir and foscarnet; VZV ATH and VZV FLO, two VZV clinical isolates; and HAdV-D37 strain.
30028308	0	72	theme	regeneration	72:83	arg1	therapy					85:91	corneal regeneration therapy	64:91	corneal regeneration therapy	64:91	Antiviral effects of Cacicol®, a heparan sulfate biomimetic for corneal regeneration therapy, for herpes simplex virus type-1 and varicella zoster virus infection.
30028308	1	73	theme	corneal	352:358	arg1	defects					371:377	persistent corneal epithelial defects	341:377	persistent corneal epithelial defects	341:377	BACKGROUND Cacicol®, a topical eye biopolymer containing a poly-carboxymethylglucose sulfate solution that is a regenerating matrix therapy agent, intended for wound healing of persistent corneal epithelial defects.
30028308	9	74	theme	antiviral	1822:1830	arg1	HSV-1					1842:1846	antiviral resistant HSV-1	1822:1846	antiviral resistant HSV-1	1822:1846	Clinical studies are necessary to determine Cacicol® for an adjunct or alternative therapy of corneal HSV-1 or VZV infection, particularly for the management of antiviral resistant HSV-1.
30028308	0	75	theme	herpes	98:103	arg1	type-1					119:124	herpes simplex virus type-1 and varicella zoster virus	98:151	type-1	119:124	Antiviral effects of Cacicol®, a heparan sulfate biomimetic for corneal regeneration therapy, for herpes simplex virus type-1 and varicella zoster virus infection.
30028308	1	76	theme	defects	371:377	arg1	healing					330:336	wound healing	324:336	wound healing of persistent corneal epithelial defects	324:377	BACKGROUND Cacicol®, a topical eye biopolymer containing a poly-carboxymethylglucose sulfate solution that is a regenerating matrix therapy agent, intended for wound healing of persistent corneal epithelial defects.
30028308	2	77	theme	cell	507:510	arg1	attachment					520:529	cell surface attachment	507:529	cell surface attachment of herpes simplex virus type-1 (HSV-1), varicella zoster virus (VZV) and human adenovirus (HAdV), three viruses associated with corneal infections	507:676	Based on the chemical composition, we hypothesized that Cacicol® may compete with natural heparan sulfate (HS) which initiates cell surface attachment of herpes simplex virus type-1 (HSV-1), varicella zoster virus (VZV) and human adenovirus (HAdV), three viruses associated with corneal infections.
30028308	9	78	theme	HSV-1	1842:1846	arg1	management					1808:1817	the management	1804:1817	the management of antiviral resistant HSV-1	1804:1846	Clinical studies are necessary to determine Cacicol® for an adjunct or alternative therapy of corneal HSV-1 or VZV infection, particularly for the management of antiviral resistant HSV-1.
30028308	2	79	theme	chemical	393:400	arg1	composition					402:412	the chemical composition	389:412	the chemical composition	389:412	Based on the chemical composition, we hypothesized that Cacicol® may compete with natural heparan sulfate (HS) which initiates cell surface attachment of herpes simplex virus type-1 (HSV-1), varicella zoster virus (VZV) and human adenovirus (HAdV), three viruses associated with corneal infections.
30028308	3	80	theme	VZV	850:852	arg1	ATH					854:856	VZV ATH and VZV FLO, two VZV clinical isolates	850:895	ATH	854:856	METHODS Cacicol® was compared to vehicle in the following viral strains: HSV-1 SC16 strain and HSV-1 PSLR, a clinical isolate highly resistant to acyclovir and foscarnet; VZV ATH and VZV FLO, two VZV clinical isolates; and HAdV-D37 strain.
30028308	5	81	theme	viral	1344:1348	arg1	replication					1350:1360	viral replication	1344:1360	viral replication	1344:1360	RESULTS The vehicle had no effect, dose-dependent effects were demonstrated when HSV-1 SC16, HSV-1 PSLR, VZV ATH and VZV FLO were inoculated in the presence of Cacicol®, inhibiting viral replication by 98.4%, 98.9%, 90.1% and 89.0%, respectively.
30028308	4	82	theme	immunostaining	1113:1126	arg1	assay					1128:1132	immunostaining assay	1113:1132	immunostaining assay on Hep-2 cells for HAdV-D37	1113:1160	Viruses in Cacicol® or vehicle were added to cells for 1 h during adsorption then viral replication was assessed by plaque reduction assays on Vero cells for HSV-1 and MeWo cells for VZV and by immunostaining assay on Hep-2 cells for HAdV-D37.
30028308	0	83	theme	Cacicol®	21:28	arg1	effects					10:16	Antiviral effects	0:16	Antiviral effects of Cacicol®, a heparan sulfate biomimetic for corneal regeneration therapy, for herpes simplex virus type-1 and varicella zoster virus infection.	0:162	Antiviral effects of Cacicol®, a heparan sulfate biomimetic for corneal regeneration therapy, for herpes simplex virus type-1 and varicella zoster virus infection.
30028308	1	84	theme	matrix	289:294	arg1	agent					304:308	a regenerating matrix therapy agent	274:308	a regenerating matrix therapy agent	274:308	BACKGROUND Cacicol®, a topical eye biopolymer containing a poly-carboxymethylglucose sulfate solution that is a regenerating matrix therapy agent, intended for wound healing of persistent corneal epithelial defects.
30028308	1	84	theme	matrix	289:294	arg1	solution					257:264	a poly-carboxymethylglucose sulfate solution	221:264	a poly-carboxymethylglucose sulfate solution that is a regenerating matrix therapy agent, intended for wound healing of persistent corneal epithelial defects	221:377	BACKGROUND Cacicol®, a topical eye biopolymer containing a poly-carboxymethylglucose sulfate solution that is a regenerating matrix therapy agent, intended for wound healing of persistent corneal epithelial defects.
30028308	4	85	theme	Hep-2	1137:1141	arg1	cells					1143:1147	Hep-2 cells	1137:1147	Hep-2 cells	1137:1147	Viruses in Cacicol® or vehicle were added to cells for 1 h during adsorption then viral replication was assessed by plaque reduction assays on Vero cells for HSV-1 and MeWo cells for VZV and by immunostaining assay on Hep-2 cells for HAdV-D37.
30028308	5	86	theme	dose-dependent	1198:1211	arg1	effects					1213:1219	dose-dependent effects	1198:1219	dose-dependent effects	1198:1219	RESULTS The vehicle had no effect, dose-dependent effects were demonstrated when HSV-1 SC16, HSV-1 PSLR, VZV ATH and VZV FLO were inoculated in the presence of Cacicol®, inhibiting viral replication by 98.4%, 98.9%, 90.1% and 89.0%, respectively.
30028308	3	87	theme	FLO	866:868	arg1	isolates					888:895	VZV ATH and VZV FLO, two VZV clinical isolates	850:895	isolates	888:895	METHODS Cacicol® was compared to vehicle in the following viral strains: HSV-1 SC16 strain and HSV-1 PSLR, a clinical isolate highly resistant to acyclovir and foscarnet; VZV ATH and VZV FLO, two VZV clinical isolates; and HAdV-D37 strain.
30028308	2	88	theme	natural	462:468	arg1	HS					487:488	HS	487:488	HS	487:488	Based on the chemical composition, we hypothesized that Cacicol® may compete with natural heparan sulfate (HS) which initiates cell surface attachment of herpes simplex virus type-1 (HSV-1), varicella zoster virus (VZV) and human adenovirus (HAdV), three viruses associated with corneal infections.
30028308	2	88	theme	natural	462:468	arg1	sulfate					478:484	natural heparan sulfate	462:484	natural heparan sulfate (HS) which initiates cell surface attachment of herpes simplex virus type-1 (HSV-1), varicella zoster virus (VZV) and human adenovirus (HAdV), three viruses associated with corneal infections	462:676	Based on the chemical composition, we hypothesized that Cacicol® may compete with natural heparan sulfate (HS) which initiates cell surface attachment of herpes simplex virus type-1 (HSV-1), varicella zoster virus (VZV) and human adenovirus (HAdV), three viruses associated with corneal infections.
30028308	4	89	from	Viruses	919:925	arg1	Cacicol®					930:937	Cacicol®	930:937	Cacicol®	930:937	Viruses in Cacicol® or vehicle were added to cells for 1 h during adsorption then viral replication was assessed by plaque reduction assays on Vero cells for HSV-1 and MeWo cells for VZV and by immunostaining assay on Hep-2 cells for HAdV-D37.
30028308	4	89	from	Viruses	919:925	arg1	vehicle					942:948	vehicle	942:948	vehicle	942:948	Viruses in Cacicol® or vehicle were added to cells for 1 h during adsorption then viral replication was assessed by plaque reduction assays on Vero cells for HSV-1 and MeWo cells for VZV and by immunostaining assay on Hep-2 cells for HAdV-D37.
30028308	3	90	theme	clinical	879:886	arg1	isolates					888:895	VZV ATH and VZV FLO, two VZV clinical isolates	850:895	isolates	888:895	METHODS Cacicol® was compared to vehicle in the following viral strains: HSV-1 SC16 strain and HSV-1 PSLR, a clinical isolate highly resistant to acyclovir and foscarnet; VZV ATH and VZV FLO, two VZV clinical isolates; and HAdV-D37 strain.
30028308	9	91	theme	Clinical	1661:1668	arg1	studies					1670:1676	Clinical studies	1661:1676	Clinical studies	1661:1676	Clinical studies are necessary to determine Cacicol® for an adjunct or alternative therapy of corneal HSV-1 or VZV infection, particularly for the management of antiviral resistant HSV-1.
30028308	8	92	from	lack	1562:1565	arg1	HAdV					1580:1583	HAdV	1580:1583	HAdV	1580:1583	The lack of effect on HAdV is probably because it is less dependent on HS interactions for cell entry.
30028308	5	93	theme	HSV-1	1256:1260	arg1	PSLR					1262:1265	HSV-1 PSLR	1256:1265	HSV-1 PSLR	1256:1265	RESULTS The vehicle had no effect, dose-dependent effects were demonstrated when HSV-1 SC16, HSV-1 PSLR, VZV ATH and VZV FLO were inoculated in the presence of Cacicol®, inhibiting viral replication by 98.4%, 98.9%, 90.1% and 89.0%, respectively.
30028308	1	94	theme	sulfate	249:255	arg1	agent					304:308	a regenerating matrix therapy agent	274:308	a regenerating matrix therapy agent	274:308	BACKGROUND Cacicol®, a topical eye biopolymer containing a poly-carboxymethylglucose sulfate solution that is a regenerating matrix therapy agent, intended for wound healing of persistent corneal epithelial defects.
30028308	1	94	theme	sulfate	249:255	arg1	solution					257:264	a poly-carboxymethylglucose sulfate solution	221:264	a poly-carboxymethylglucose sulfate solution that is a regenerating matrix therapy agent, intended for wound healing of persistent corneal epithelial defects	221:377	BACKGROUND Cacicol®, a topical eye biopolymer containing a poly-carboxymethylglucose sulfate solution that is a regenerating matrix therapy agent, intended for wound healing of persistent corneal epithelial defects.
29458505	6	0	theme	unidentified	838:849	arg1	phospholipid					851:862	one unidentified phospholipid	834:862	one unidentified phospholipid	834:862	The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two phosphatidylinositol mannosides, one unidentified phospholipid, three unidentified glycolipids and one unidentified lipid.
29458505	13	1	theme	phylogenetic	1568:1579	arg1	analysis					1581:1588	the phylogenetic analysis	1564:1588	the phylogenetic analysis	1564:1588	On the basis of the phylogenetic analysis, chemotaxonomic data, physiological characteristics and DNA-DNA hybridization data, strain THG-T121T represents a novel species of the genus Actinotalea, for which the name Actinotaleasolisilvae sp.
29458505	12	2	dep	C.	1429:1430	arg1	carbonis					1432:1439	carbonis	1432:1439	carbonis	1432:1439	DNA-DNA hybridization values between strain THG-T121T and A. ferrariae KCTC 29134T, A. fermentans KCTC 3251T and C. carbonis KCTC 19824T were 30.2 % (27.3 %, reciprocal analysis), 28.4 %, (17.3 %) and 16.9 %, (9.3 %), respectively.
29458505	13	3	theme	genus	1725:1729	arg1	Actinotalea					1731:1741	the genus Actinotalea	1721:1741	the genus Actinotalea	1721:1741	On the basis of the phylogenetic analysis, chemotaxonomic data, physiological characteristics and DNA-DNA hybridization data, strain THG-T121T represents a novel species of the genus Actinotalea, for which the name Actinotaleasolisilvae sp.
29458505	4	4	theme	Actinotalea	507:517	arg1	29134T					490:495	Actinotalea ferrariae KCTC 29134T	463:495	Actinotalea ferrariae KCTC 29134T (97.9 %)	463:504	Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-T121T were identified as Actinotalea ferrariae KCTC 29134T (97.9 %), Actinotalea fermentans KCTC 3251T (97.3 %), Cellulomonas carbonis KCTC 19824T (97.2 %).
29458505	4	4	theme	Actinotalea	507:517	arg1	3251T					535:539	Actinotalea fermentans KCTC 3251T	507:539	Actinotalea fermentans KCTC 3251T (97.3 %)	507:548	Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-T121T were identified as Actinotalea ferrariae KCTC 29134T (97.9 %), Actinotalea fermentans KCTC 3251T (97.3 %), Cellulomonas carbonis KCTC 19824T (97.2 %).
29458505	4	4	theme	Actinotalea	507:517	arg1	%					547:547	97.3 %	542:547	97.3 %	542:547	Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-T121T were identified as Actinotalea ferrariae KCTC 29134T (97.9 %), Actinotalea fermentans KCTC 3251T (97.3 %), Cellulomonas carbonis KCTC 19824T (97.2 %).
29458505	11	5	theme	DNA	1265:1267	arg1	G+C content					1269:1279	The DNA G+C content	1261:1279	The DNA G+C content of strain THG-T121T	1261:1299	The DNA G+C content of strain THG-T121T was 72.4 mol%.
29458505	11	5	theme	DNA	1265:1267	arg1	%					1313:1313	72.4 mol%	1305:1313	72.4 mol%	1305:1313	The DNA G+C content of strain THG-T121T was 72.4 mol%.
29458505	2	6	theme	non-motile	141:150	arg1	actinobacterium					173:187	A Gram-stain-positive, aerobic, non-motile and short-rod-shaped actinobacterium	109:187	A Gram-stain-positive, aerobic, non-motile and short-rod-shaped actinobacterium	109:187	A Gram-stain-positive, aerobic, non-motile and short-rod-shaped actinobacterium, designated THG-T121T, was isolated from forest soil.
29458505	6	7	theme	phosphatidylinositol	801:820	arg1	mannosides					822:831	diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two phosphatidylinositol mannosides	729:831	mannosides	822:831	The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two phosphatidylinositol mannosides, one unidentified phospholipid, three unidentified glycolipids and one unidentified lipid.
29458505	6	7	theme	phosphatidylinositol	801:820	arg1	lipids					717:722	The polar lipids	707:722	The polar lipids	707:722	The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two phosphatidylinositol mannosides, one unidentified phospholipid, three unidentified glycolipids and one unidentified lipid.
29458505	4	8	theme	carbonis	564:571	arg1	%					591:591	97.2 %	586:591	97.2 %	586:591	Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-T121T were identified as Actinotalea ferrariae KCTC 29134T (97.9 %), Actinotalea fermentans KCTC 3251T (97.3 %), Cellulomonas carbonis KCTC 19824T (97.2 %).
29458505	4	8	theme	carbonis	564:571	arg1	29134T					490:495	Actinotalea ferrariae KCTC 29134T	463:495	Actinotalea ferrariae KCTC 29134T (97.9 %)	463:504	Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-T121T were identified as Actinotalea ferrariae KCTC 29134T (97.9 %), Actinotalea fermentans KCTC 3251T (97.3 %), Cellulomonas carbonis KCTC 19824T (97.2 %).
29458505	4	8	theme	carbonis	564:571	arg1	19824T					578:583	Cellulomonas carbonis KCTC 19824T	551:583	Cellulomonas carbonis KCTC 19824T (97.2 %)	551:592	Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-T121T were identified as Actinotalea ferrariae KCTC 29134T (97.9 %), Actinotalea fermentans KCTC 3251T (97.3 %), Cellulomonas carbonis KCTC 19824T (97.2 %).
29458505	2	9	theme	aerobic	132:138	arg1	actinobacterium					173:187	A Gram-stain-positive, aerobic, non-motile and short-rod-shaped actinobacterium	109:187	A Gram-stain-positive, aerobic, non-motile and short-rod-shaped actinobacterium	109:187	A Gram-stain-positive, aerobic, non-motile and short-rod-shaped actinobacterium, designated THG-T121T, was isolated from forest soil.
29458505	13	10	theme	novel	1704:1708	arg1	species					1710:1716	a novel species	1702:1716	a novel species	1702:1716	On the basis of the phylogenetic analysis, chemotaxonomic data, physiological characteristics and DNA-DNA hybridization data, strain THG-T121T represents a novel species of the genus Actinotalea, for which the name Actinotaleasolisilvae sp.
29458505	12	11	dep	%	1510:1510	arg1	16.9 					1517:1521	16.9 	1517:1521	16.9 	1517:1521	DNA-DNA hybridization values between strain THG-T121T and A. ferrariae KCTC 29134T, A. fermentans KCTC 3251T and C. carbonis KCTC 19824T were 30.2 % (27.3 %, reciprocal analysis), 28.4 %, (17.3 %) and 16.9 %, (9.3 %), respectively.
29458505	6	12	theme	unidentified	904:915	arg1	lipid					917:921	one unidentified lipid	900:921	one unidentified lipid	900:921	The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two phosphatidylinositol mannosides, one unidentified phospholipid, three unidentified glycolipids and one unidentified lipid.
29458505	3	13	theme	optimum	272:278	arg1	10-40 °C					262:269	10-40 °C	262:269	10-40 °C (optimum 28-30 °C)	262:288	Growth occurred at 10-40 °C (optimum 28-30 °C), at pH 6-8 (optimum 7) and at 0-4 % NaCl (optimum 1 %).
29458505	3	13	theme	optimum	272:278	arg1	28-30 °C					280:287	optimum 28-30 °C	272:287	optimum 28-30 °C	272:287	Growth occurred at 10-40 °C (optimum 28-30 °C), at pH 6-8 (optimum 7) and at 0-4 % NaCl (optimum 1 %).
29458505	8	14	dep	 0	1016:1017	arg1	 0					1090:1091	 0	1090:1091	 0	1090:1091	The major fatty acids were anteiso-C15 : 0, anteiso-C15 : 1 A, C16 : 0, iso-C16 : 0, anteiso-C17 : 0 and iso-C17 : 0.
29458505	8	14	dep	 0	1016:1017	arg1	iso-C16 					1048:1055	iso-C16 	1048:1055	iso-C16 	1048:1055	The major fatty acids were anteiso-C15 : 0, anteiso-C15 : 1 A, C16 : 0, iso-C16 : 0, anteiso-C17 : 0 and iso-C17 : 0.
29458505	8	14	dep	 0	1016:1017	arg1	 0					1074:1075	 0	1074:1075	 0	1074:1075	The major fatty acids were anteiso-C15 : 0, anteiso-C15 : 1 A, C16 : 0, iso-C16 : 0, anteiso-C17 : 0 and iso-C17 : 0.
29458505	8	14	dep	 0	1016:1017	arg1	A					1036:1036	 1 A	1033:1036	 1 A	1033:1036	The major fatty acids were anteiso-C15 : 0, anteiso-C15 : 1 A, C16 : 0, iso-C16 : 0, anteiso-C17 : 0 and iso-C17 : 0.
29458505	8	14	dep	 0	1016:1017	arg1	iso-C17 					1081:1088	iso-C17 	1081:1088	iso-C17 	1081:1088	The major fatty acids were anteiso-C15 : 0, anteiso-C15 : 1 A, C16 : 0, iso-C16 : 0, anteiso-C17 : 0 and iso-C17 : 0.
29458505	8	14	dep	 0	1016:1017	arg1	C16 					1039:1042	C16 	1039:1042	C16 	1039:1042	The major fatty acids were anteiso-C15 : 0, anteiso-C15 : 1 A, C16 : 0, iso-C16 : 0, anteiso-C17 : 0 and iso-C17 : 0.
29458505	8	14	dep	 0	1016:1017	arg1	 0					1057:1058	 0	1057:1058	 0	1057:1058	The major fatty acids were anteiso-C15 : 0, anteiso-C15 : 1 A, C16 : 0, iso-C16 : 0, anteiso-C17 : 0 and iso-C17 : 0.
29458505	5	15	theme	16S	595:597	arg1	similarities					618:629	16S rRNA gene sequence similarities	595:629	16S rRNA gene sequence similarities among strain THG-T121T	595:652	16S rRNA gene sequence similarities among strain THG-T121T and other recognized species were lower than 97.0 %.
29458505	4	16	theme	gene	364:367	arg1	analysis					378:385	16S rRNA gene sequence analysis	355:385	16S rRNA gene sequence analysis	355:385	Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-T121T were identified as Actinotalea ferrariae KCTC 29134T (97.9 %), Actinotalea fermentans KCTC 3251T (97.3 %), Cellulomonas carbonis KCTC 19824T (97.2 %).
29458505	8	17	dep	anteiso-C15 	1003:1014	arg1	anteiso-C15 					1003:1014	anteiso-C15 	1003:1014	anteiso-C15 	1003:1014	The major fatty acids were anteiso-C15 : 0, anteiso-C15 : 1 A, C16 : 0, iso-C16 : 0, anteiso-C17 : 0 and iso-C17 : 0.
29458505	8	17	dep	anteiso-C15 	1003:1014	arg1	acids					992:996	The major fatty acids	976:996	The major fatty acids	976:996	The major fatty acids were anteiso-C15 : 0, anteiso-C15 : 1 A, C16 : 0, iso-C16 : 0, anteiso-C17 : 0 and iso-C17 : 0.
29458505	8	17	dep	anteiso-C15 	1003:1014	arg1	 0					1016:1017	 0	1016:1017	 0	1016:1017	The major fatty acids were anteiso-C15 : 0, anteiso-C15 : 1 A, C16 : 0, iso-C16 : 0, anteiso-C17 : 0 and iso-C17 : 0.
29458505	5	18	theme	gene	604:607	arg1	similarities					618:629	16S rRNA gene sequence similarities	595:629	16S rRNA gene sequence similarities among strain THG-T121T	595:652	16S rRNA gene sequence similarities among strain THG-T121T and other recognized species were lower than 97.0 %.
29458505	2	19	theme	forest	230:235	arg1	soil					237:240	forest soil	230:240	forest soil	230:240	A Gram-stain-positive, aerobic, non-motile and short-rod-shaped actinobacterium, designated THG-T121T, was isolated from forest soil.
29458505	6	20	theme	unidentified	871:882	arg1	glycolipids					884:894	three unidentified glycolipids	865:894	three unidentified glycolipids	865:894	The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two phosphatidylinositol mannosides, one unidentified phospholipid, three unidentified glycolipids and one unidentified lipid.
29458505	9	21	theme	whole-cell	1098:1107	arg1	rhamnose					1141:1148	rhamnose	1141:1148	rhamnose	1141:1148	The whole-cell sugars of strain THG-T121T were rhamnose, ribose, mannose and glucose.
29458505	9	21	theme	whole-cell	1098:1107	arg1	sugars					1109:1114	The whole-cell sugars	1094:1114	The whole-cell sugars of strain THG-T121T	1094:1134	The whole-cell sugars of strain THG-T121T were rhamnose, ribose, mannose and glucose.
29458505	12	22	theme	%	1510:1510	arg1	%					1522:1522	(17.3 %) and 16.9 %	1504:1522	%	1522:1522	DNA-DNA hybridization values between strain THG-T121T and A. ferrariae KCTC 29134T, A. fermentans KCTC 3251T and C. carbonis KCTC 19824T were 30.2 % (27.3 %, reciprocal analysis), 28.4 %, (17.3 %) and 16.9 %, (9.3 %), respectively.
29458505	12	22	theme	%	1510:1510	arg1	%					1463:1463	30.2 %	1458:1463	30.2 % (27.3 %, reciprocal analysis)	1458:1493	DNA-DNA hybridization values between strain THG-T121T and A. ferrariae KCTC 29134T, A. fermentans KCTC 3251T and C. carbonis KCTC 19824T were 30.2 % (27.3 %, reciprocal analysis), 28.4 %, (17.3 %) and 16.9 %, (9.3 %), respectively.
29458505	7	23	theme	isoprenoid	928:937	arg1	menaquinone					951:961	menaquinone	951:961	menaquinone (MK-10(H4))	951:973	The isoprenoid quinone was menaquinone (MK-10(H4)).
29458505	7	23	theme	isoprenoid	928:937	arg1	quinone					939:945	The isoprenoid quinone	924:945	The isoprenoid quinone	924:945	The isoprenoid quinone was menaquinone (MK-10(H4)).
29458505	12	24	theme	A.	1400:1401	arg1	3251T					1419:1423	A. fermentans KCTC 3251T	1400:1423	A. fermentans KCTC 3251T	1400:1423	DNA-DNA hybridization values between strain THG-T121T and A. ferrariae KCTC 29134T, A. fermentans KCTC 3251T and C. carbonis KCTC 19824T were 30.2 % (27.3 %, reciprocal analysis), 28.4 %, (17.3 %) and 16.9 %, (9.3 %), respectively.
29458505	4	25	theme	16S	355:357	arg1	analysis					378:385	16S rRNA gene sequence analysis	355:385	16S rRNA gene sequence analysis	355:385	Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-T121T were identified as Actinotalea ferrariae KCTC 29134T (97.9 %), Actinotalea fermentans KCTC 3251T (97.3 %), Cellulomonas carbonis KCTC 19824T (97.2 %).
29458505	9	26	theme	THG-T121T	1126:1134	arg1	rhamnose					1141:1148	rhamnose	1141:1148	rhamnose	1141:1148	The whole-cell sugars of strain THG-T121T were rhamnose, ribose, mannose and glucose.
29458505	9	26	theme	THG-T121T	1126:1134	arg1	sugars					1109:1114	The whole-cell sugars	1094:1114	The whole-cell sugars of strain THG-T121T	1094:1134	The whole-cell sugars of strain THG-T121T were rhamnose, ribose, mannose and glucose.
29458505	12	27	theme	KCTC	1414:1417	arg1	3251T					1419:1423	A. fermentans KCTC 3251T	1400:1423	A. fermentans KCTC 3251T	1400:1423	DNA-DNA hybridization values between strain THG-T121T and A. ferrariae KCTC 29134T, A. fermentans KCTC 3251T and C. carbonis KCTC 19824T were 30.2 % (27.3 %, reciprocal analysis), 28.4 %, (17.3 %) and 16.9 %, (9.3 %), respectively.
29458505	12	28	dep	%	1463:1463	arg1	%					1471:1471	27.3 %	1466:1471	27.3 %	1466:1471	DNA-DNA hybridization values between strain THG-T121T and A. ferrariae KCTC 29134T, A. fermentans KCTC 3251T and C. carbonis KCTC 19824T were 30.2 % (27.3 %, reciprocal analysis), 28.4 %, (17.3 %) and 16.9 %, (9.3 %), respectively.
29458505	12	28	dep	%	1463:1463	arg1	analysis					1485:1492	reciprocal analysis	1474:1492	reciprocal analysis	1474:1492	DNA-DNA hybridization values between strain THG-T121T and A. ferrariae KCTC 29134T, A. fermentans KCTC 3251T and C. carbonis KCTC 19824T were 30.2 % (27.3 %, reciprocal analysis), 28.4 %, (17.3 %) and 16.9 %, (9.3 %), respectively.
29458505	12	29	dep	A.	1374:1375	arg1	ferrariae					1377:1385	ferrariae	1377:1385	ferrariae	1377:1385	DNA-DNA hybridization values between strain THG-T121T and A. ferrariae KCTC 29134T, A. fermentans KCTC 3251T and C. carbonis KCTC 19824T were 30.2 % (27.3 %, reciprocal analysis), 28.4 %, (17.3 %) and 16.9 %, (9.3 %), respectively.
29458505	5	30	theme	recognized	664:673	arg1	species					675:681	other recognized species	658:681	other recognized species	658:681	16S rRNA gene sequence similarities among strain THG-T121T and other recognized species were lower than 97.0 %.
29458505	4	31	theme	THG-T121T	434:442	arg1	29134T					490:495	Actinotalea ferrariae KCTC 29134T	463:495	Actinotalea ferrariae KCTC 29134T (97.9 %)	463:504	Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-T121T were identified as Actinotalea ferrariae KCTC 29134T (97.9 %), Actinotalea fermentans KCTC 3251T (97.3 %), Cellulomonas carbonis KCTC 19824T (97.2 %).
29458505	4	31	theme	THG-T121T	434:442	arg1	neighbours					413:422	the nearest phylogenetic neighbours	388:422	the nearest phylogenetic neighbours of strain THG-T121T	388:442	Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-T121T were identified as Actinotalea ferrariae KCTC 29134T (97.9 %), Actinotalea fermentans KCTC 3251T (97.3 %), Cellulomonas carbonis KCTC 19824T (97.2 %).
29458505	11	32	theme	THG-T121T	1291:1299	arg1	G+C content					1269:1279	The DNA G+C content	1261:1279	The DNA G+C content of strain THG-T121T	1261:1299	The DNA G+C content of strain THG-T121T was 72.4 mol%.
29458505	11	32	theme	THG-T121T	1291:1299	arg1	%					1313:1313	72.4 mol%	1305:1313	72.4 mol%	1305:1313	The DNA G+C content of strain THG-T121T was 72.4 mol%.
29458505	5	33	theme	strain	637:642	arg1	THG-T121T					644:652	strain THG-T121T	637:652	strain THG-T121T	637:652	16S rRNA gene sequence similarities among strain THG-T121T and other recognized species were lower than 97.0 %.
29458505	1	34	theme	emended	63:69	arg1	description					71:81	emended description	63:81	emended description	63:81	nov., isolated from forest soil and emended description of the genus Actinotalea.
29458505	4	35	theme	phylogenetic	400:411	arg1	29134T					490:495	Actinotalea ferrariae KCTC 29134T	463:495	Actinotalea ferrariae KCTC 29134T (97.9 %)	463:504	Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-T121T were identified as Actinotalea ferrariae KCTC 29134T (97.9 %), Actinotalea fermentans KCTC 3251T (97.3 %), Cellulomonas carbonis KCTC 19824T (97.2 %).
29458505	4	35	theme	phylogenetic	400:411	arg1	neighbours					413:422	the nearest phylogenetic neighbours	388:422	the nearest phylogenetic neighbours of strain THG-T121T	388:442	Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-T121T were identified as Actinotalea ferrariae KCTC 29134T (97.9 %), Actinotalea fermentans KCTC 3251T (97.3 %), Cellulomonas carbonis KCTC 19824T (97.2 %).
29458505	0	36	theme	Actinotalea	0:10	arg1	sp					23:24	Actinotalea solisilvae sp	0:24	Actinotalea solisilvae sp.	0:25	Actinotalea solisilvae sp.
29458505	8	37	theme	major	980:984	arg1	anteiso-C15 					1003:1014	anteiso-C15 	1003:1014	anteiso-C15 	1003:1014	The major fatty acids were anteiso-C15 : 0, anteiso-C15 : 1 A, C16 : 0, iso-C16 : 0, anteiso-C17 : 0 and iso-C17 : 0.
29458505	8	37	theme	major	980:984	arg1	acids					992:996	The major fatty acids	976:996	The major fatty acids	976:996	The major fatty acids were anteiso-C15 : 0, anteiso-C15 : 1 A, C16 : 0, iso-C16 : 0, anteiso-C17 : 0 and iso-C17 : 0.
29458505	8	37	theme	major	980:984	arg1	 0					1016:1017	 0	1016:1017	 0	1016:1017	The major fatty acids were anteiso-C15 : 0, anteiso-C15 : 1 A, C16 : 0, iso-C16 : 0, anteiso-C17 : 0 and iso-C17 : 0.
29458505	15	38	theme	type	1811:1814	arg1	THG-T121T					1826:1834	THG-T121T	1826:1834	THG-T121T (=KACC 19191T=CGMCC 4.7389T)	1826:1863	The type strain is THG-T121T (=KACC 19191T=CGMCC 4.7389T).
29458505	15	38	theme	type	1811:1814	arg1	strain					1816:1821	The type strain	1807:1821	The type strain	1807:1821	The type strain is THG-T121T (=KACC 19191T=CGMCC 4.7389T).
29458505	3	39	theme	%	324:324	arg1	%					342:342	optimum 1 %	332:342	optimum 1 %	332:342	Growth occurred at 10-40 °C (optimum 28-30 °C), at pH 6-8 (optimum 7) and at 0-4 % NaCl (optimum 1 %).
29458505	3	39	theme	%	324:324	arg1	 NaCl					325:329	0-4 % NaCl	320:329	0-4 % NaCl (optimum 1 %)	320:343	Growth occurred at 10-40 °C (optimum 28-30 °C), at pH 6-8 (optimum 7) and at 0-4 % NaCl (optimum 1 %).
29458505	4	40	theme	KCTC	485:488	arg1	29134T					490:495	Actinotalea ferrariae KCTC 29134T	463:495	Actinotalea ferrariae KCTC 29134T (97.9 %)	463:504	Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-T121T were identified as Actinotalea ferrariae KCTC 29134T (97.9 %), Actinotalea fermentans KCTC 3251T (97.3 %), Cellulomonas carbonis KCTC 19824T (97.2 %).
29458505	4	40	theme	KCTC	485:488	arg1	%					503:503	97.9 %	498:503	97.9 %	498:503	Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-T121T were identified as Actinotalea ferrariae KCTC 29134T (97.9 %), Actinotalea fermentans KCTC 3251T (97.3 %), Cellulomonas carbonis KCTC 19824T (97.2 %).
29458505	4	40	theme	KCTC	485:488	arg1	neighbours					413:422	the nearest phylogenetic neighbours	388:422	the nearest phylogenetic neighbours of strain THG-T121T	388:442	Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-T121T were identified as Actinotalea ferrariae KCTC 29134T (97.9 %), Actinotalea fermentans KCTC 3251T (97.3 %), Cellulomonas carbonis KCTC 19824T (97.2 %).
29458505	4	40	theme	KCTC	485:488	arg1	3251T					535:539	Actinotalea fermentans KCTC 3251T	507:539	Actinotalea fermentans KCTC 3251T (97.3 %)	507:548	Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-T121T were identified as Actinotalea ferrariae KCTC 29134T (97.9 %), Actinotalea fermentans KCTC 3251T (97.3 %), Cellulomonas carbonis KCTC 19824T (97.2 %).
29458505	4	40	theme	KCTC	485:488	arg1	19824T					578:583	Cellulomonas carbonis KCTC 19824T	551:583	Cellulomonas carbonis KCTC 19824T (97.2 %)	551:592	Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-T121T were identified as Actinotalea ferrariae KCTC 29134T (97.9 %), Actinotalea fermentans KCTC 3251T (97.3 %), Cellulomonas carbonis KCTC 19824T (97.2 %).
29458505	1	41	theme	genus	90:94	arg1	Actinotalea					96:106	the genus Actinotalea	86:106	the genus Actinotalea	86:106	nov., isolated from forest soil and emended description of the genus Actinotalea.
29458505	11	42	theme	72.4 mol	1305:1312	arg1	G+C content					1269:1279	The DNA G+C content	1261:1279	The DNA G+C content of strain THG-T121T	1261:1299	The DNA G+C content of strain THG-T121T was 72.4 mol%.
29458505	11	42	theme	72.4 mol	1305:1312	arg1	%					1313:1313	72.4 mol%	1305:1313	72.4 mol%	1305:1313	The DNA G+C content of strain THG-T121T was 72.4 mol%.
29458505	12	43	theme	A.	1374:1375	arg1	29134T					1392:1397	A. ferrariae KCTC 29134T	1374:1397	A. ferrariae KCTC 29134T	1374:1397	DNA-DNA hybridization values between strain THG-T121T and A. ferrariae KCTC 29134T, A. fermentans KCTC 3251T and C. carbonis KCTC 19824T were 30.2 % (27.3 %, reciprocal analysis), 28.4 %, (17.3 %) and 16.9 %, (9.3 %), respectively.
29458505	12	44	theme	hybridization	1324:1336	arg1	values					1338:1343	DNA-DNA hybridization values	1316:1343	DNA-DNA hybridization values between strain THG-T121T and A. ferrariae KCTC 29134T, A. fermentans KCTC 3251T and C. carbonis KCTC 19824T	1316:1451	DNA-DNA hybridization values between strain THG-T121T and A. ferrariae KCTC 29134T, A. fermentans KCTC 3251T and C. carbonis KCTC 19824T were 30.2 % (27.3 %, reciprocal analysis), 28.4 %, (17.3 %) and 16.9 %, (9.3 %), respectively.
29458505	4	45	theme	Actinotalea	463:473	arg1	29134T					490:495	Actinotalea ferrariae KCTC 29134T	463:495	Actinotalea ferrariae KCTC 29134T (97.9 %)	463:504	Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-T121T were identified as Actinotalea ferrariae KCTC 29134T (97.9 %), Actinotalea fermentans KCTC 3251T (97.3 %), Cellulomonas carbonis KCTC 19824T (97.2 %).
29458505	4	45	theme	Actinotalea	463:473	arg1	%					503:503	97.9 %	498:503	97.9 %	498:503	Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-T121T were identified as Actinotalea ferrariae KCTC 29134T (97.9 %), Actinotalea fermentans KCTC 3251T (97.3 %), Cellulomonas carbonis KCTC 19824T (97.2 %).
29458505	4	45	theme	Actinotalea	463:473	arg1	neighbours					413:422	the nearest phylogenetic neighbours	388:422	the nearest phylogenetic neighbours of strain THG-T121T	388:442	Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-T121T were identified as Actinotalea ferrariae KCTC 29134T (97.9 %), Actinotalea fermentans KCTC 3251T (97.3 %), Cellulomonas carbonis KCTC 19824T (97.2 %).
29458505	4	45	theme	Actinotalea	463:473	arg1	3251T					535:539	Actinotalea fermentans KCTC 3251T	507:539	Actinotalea fermentans KCTC 3251T (97.3 %)	507:548	Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-T121T were identified as Actinotalea ferrariae KCTC 29134T (97.9 %), Actinotalea fermentans KCTC 3251T (97.3 %), Cellulomonas carbonis KCTC 19824T (97.2 %).
29458505	4	45	theme	Actinotalea	463:473	arg1	19824T					578:583	Cellulomonas carbonis KCTC 19824T	551:583	Cellulomonas carbonis KCTC 19824T (97.2 %)	551:592	Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-T121T were identified as Actinotalea ferrariae KCTC 29134T (97.9 %), Actinotalea fermentans KCTC 3251T (97.3 %), Cellulomonas carbonis KCTC 19824T (97.2 %).
29458505	4	46	theme	fermentans	519:528	arg1	29134T					490:495	Actinotalea ferrariae KCTC 29134T	463:495	Actinotalea ferrariae KCTC 29134T (97.9 %)	463:504	Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-T121T were identified as Actinotalea ferrariae KCTC 29134T (97.9 %), Actinotalea fermentans KCTC 3251T (97.3 %), Cellulomonas carbonis KCTC 19824T (97.2 %).
29458505	4	46	theme	fermentans	519:528	arg1	3251T					535:539	Actinotalea fermentans KCTC 3251T	507:539	Actinotalea fermentans KCTC 3251T (97.3 %)	507:548	Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-T121T were identified as Actinotalea ferrariae KCTC 29134T (97.9 %), Actinotalea fermentans KCTC 3251T (97.3 %), Cellulomonas carbonis KCTC 19824T (97.2 %).
29458505	4	46	theme	fermentans	519:528	arg1	%					547:547	97.3 %	542:547	97.3 %	542:547	Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-T121T were identified as Actinotalea ferrariae KCTC 29134T (97.9 %), Actinotalea fermentans KCTC 3251T (97.3 %), Cellulomonas carbonis KCTC 19824T (97.2 %).
29458505	10	47	theme	strain	1206:1211	arg1	THG-T121T					1213:1221	strain THG-T121T	1206:1221	strain THG-T121T	1206:1221	The peptidoglycan type of strain THG-T121T is A4β, containing l-Orn-D-Ser-L-Asp.
29458505	8	48	theme	 1	1033:1034	arg1	C16 					1039:1042	C16 	1039:1042	C16 	1039:1042	The major fatty acids were anteiso-C15 : 0, anteiso-C15 : 1 A, C16 : 0, iso-C16 : 0, anteiso-C17 : 0 and iso-C17 : 0.
29458505	8	48	theme	 1	1033:1034	arg1	A					1036:1036	 1 A	1033:1036	 1 A	1033:1036	The major fatty acids were anteiso-C15 : 0, anteiso-C15 : 1 A, C16 : 0, iso-C16 : 0, anteiso-C17 : 0 and iso-C17 : 0.
29458505	8	48	theme	 1	1033:1034	arg1	iso-C16 					1048:1055	iso-C16 	1048:1055	iso-C16 	1048:1055	The major fatty acids were anteiso-C15 : 0, anteiso-C15 : 1 A, C16 : 0, iso-C16 : 0, anteiso-C17 : 0 and iso-C17 : 0.
29458505	15	49	theme	19191T=CGMCC	1843:1854	arg1	4.7389T					1856:1862	=KACC 19191T=CGMCC 4.7389T	1837:1862	=KACC 19191T=CGMCC 4.7389T	1837:1862	The type strain is THG-T121T (=KACC 19191T=CGMCC 4.7389T).
29458505	15	49	theme	19191T=CGMCC	1843:1854	arg1	THG-T121T					1826:1834	THG-T121T	1826:1834	THG-T121T (=KACC 19191T=CGMCC 4.7389T)	1826:1863	The type strain is THG-T121T (=KACC 19191T=CGMCC 4.7389T).
29458505	13	50	dep	analysis	1581:1588	arg1	data					1668:1671	data	1668:1671	data	1668:1671	On the basis of the phylogenetic analysis, chemotaxonomic data, physiological characteristics and DNA-DNA hybridization data, strain THG-T121T represents a novel species of the genus Actinotalea, for which the name Actinotaleasolisilvae sp.
29458505	13	50	dep	analysis	1581:1588	arg1	the					1551:1553	the	1551:1553	the	1551:1553	On the basis of the phylogenetic analysis, chemotaxonomic data, physiological characteristics and DNA-DNA hybridization data, strain THG-T121T represents a novel species of the genus Actinotalea, for which the name Actinotaleasolisilvae sp.
29458505	13	50	dep	analysis	1581:1588	arg1	basis					1555:1559	basis	1555:1559	basis	1555:1559	On the basis of the phylogenetic analysis, chemotaxonomic data, physiological characteristics and DNA-DNA hybridization data, strain THG-T121T represents a novel species of the genus Actinotalea, for which the name Actinotaleasolisilvae sp.
29458505	13	51	theme	Actinotaleasolisilvae	1763:1783	arg1	sp					1785:1786	the name Actinotaleasolisilvae sp	1754:1786	the name Actinotaleasolisilvae sp	1754:1786	On the basis of the phylogenetic analysis, chemotaxonomic data, physiological characteristics and DNA-DNA hybridization data, strain THG-T121T represents a novel species of the genus Actinotalea, for which the name Actinotaleasolisilvae sp.
29458505	13	52	theme	Actinotalea	1731:1741	arg1	species					1710:1716	a novel species	1702:1716	a novel species	1702:1716	On the basis of the phylogenetic analysis, chemotaxonomic data, physiological characteristics and DNA-DNA hybridization data, strain THG-T121T represents a novel species of the genus Actinotalea, for which the name Actinotaleasolisilvae sp.
29458505	4	53	theme	Cellulomonas	551:562	arg1	%					591:591	97.2 %	586:591	97.2 %	586:591	Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-T121T were identified as Actinotalea ferrariae KCTC 29134T (97.9 %), Actinotalea fermentans KCTC 3251T (97.3 %), Cellulomonas carbonis KCTC 19824T (97.2 %).
29458505	4	53	theme	Cellulomonas	551:562	arg1	29134T					490:495	Actinotalea ferrariae KCTC 29134T	463:495	Actinotalea ferrariae KCTC 29134T (97.9 %)	463:504	Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-T121T were identified as Actinotalea ferrariae KCTC 29134T (97.9 %), Actinotalea fermentans KCTC 3251T (97.3 %), Cellulomonas carbonis KCTC 19824T (97.2 %).
29458505	4	53	theme	Cellulomonas	551:562	arg1	19824T					578:583	Cellulomonas carbonis KCTC 19824T	551:583	Cellulomonas carbonis KCTC 19824T (97.2 %)	551:592	Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-T121T were identified as Actinotalea ferrariae KCTC 29134T (97.9 %), Actinotalea fermentans KCTC 3251T (97.3 %), Cellulomonas carbonis KCTC 19824T (97.2 %).
29458505	6	54	theme	diphosphatidylglycerol	729:750	arg1	mannosides					822:831	diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two phosphatidylinositol mannosides	729:831	mannosides	822:831	The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two phosphatidylinositol mannosides, one unidentified phospholipid, three unidentified glycolipids and one unidentified lipid.
29458505	6	54	theme	diphosphatidylglycerol	729:750	arg1	lipids					717:722	The polar lipids	707:722	The polar lipids	707:722	The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two phosphatidylinositol mannosides, one unidentified phospholipid, three unidentified glycolipids and one unidentified lipid.
29458505	4	55	theme	KCTC	573:576	arg1	%					591:591	97.2 %	586:591	97.2 %	586:591	Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-T121T were identified as Actinotalea ferrariae KCTC 29134T (97.9 %), Actinotalea fermentans KCTC 3251T (97.3 %), Cellulomonas carbonis KCTC 19824T (97.2 %).
29458505	4	55	theme	KCTC	573:576	arg1	29134T					490:495	Actinotalea ferrariae KCTC 29134T	463:495	Actinotalea ferrariae KCTC 29134T (97.9 %)	463:504	Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-T121T were identified as Actinotalea ferrariae KCTC 29134T (97.9 %), Actinotalea fermentans KCTC 3251T (97.3 %), Cellulomonas carbonis KCTC 19824T (97.2 %).
29458505	4	55	theme	KCTC	573:576	arg1	19824T					578:583	Cellulomonas carbonis KCTC 19824T	551:583	Cellulomonas carbonis KCTC 19824T (97.2 %)	551:592	Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-T121T were identified as Actinotalea ferrariae KCTC 29134T (97.9 %), Actinotalea fermentans KCTC 3251T (97.3 %), Cellulomonas carbonis KCTC 19824T (97.2 %).
29458505	4	56	theme	sequence	369:376	arg1	analysis					378:385	16S rRNA gene sequence analysis	355:385	16S rRNA gene sequence analysis	355:385	Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-T121T were identified as Actinotalea ferrariae KCTC 29134T (97.9 %), Actinotalea fermentans KCTC 3251T (97.3 %), Cellulomonas carbonis KCTC 19824T (97.2 %).
29458505	5	57	theme	rRNA	599:602	arg1	similarities					618:629	16S rRNA gene sequence similarities	595:629	16S rRNA gene sequence similarities among strain THG-T121T	595:652	16S rRNA gene sequence similarities among strain THG-T121T and other recognized species were lower than 97.0 %.
29458505	12	58	theme	C.	1429:1430	arg1	19824T					1446:1451	C. carbonis KCTC 19824T	1429:1451	C. carbonis KCTC 19824T	1429:1451	DNA-DNA hybridization values between strain THG-T121T and A. ferrariae KCTC 29134T, A. fermentans KCTC 3251T and C. carbonis KCTC 19824T were 30.2 % (27.3 %, reciprocal analysis), 28.4 %, (17.3 %) and 16.9 %, (9.3 %), respectively.
29458505	1	59	theme	Actinotalea	96:106	arg1	soil					54:57	forest soil	47:57	forest soil	47:57	nov., isolated from forest soil and emended description of the genus Actinotalea.
29458505	1	59	theme	Actinotalea	96:106	arg1	description					71:81	emended description	63:81	emended description	63:81	nov., isolated from forest soil and emended description of the genus Actinotalea.
29458505	12	60	theme	KCTC	1441:1444	arg1	19824T					1446:1451	C. carbonis KCTC 19824T	1429:1451	C. carbonis KCTC 19824T	1429:1451	DNA-DNA hybridization values between strain THG-T121T and A. ferrariae KCTC 29134T, A. fermentans KCTC 3251T and C. carbonis KCTC 19824T were 30.2 % (27.3 %, reciprocal analysis), 28.4 %, (17.3 %) and 16.9 %, (9.3 %), respectively.
29458505	13	61	theme	strain	1674:1679	arg1	THG-T121T					1681:1689	strain THG-T121T	1674:1689	strain THG-T121T	1674:1689	On the basis of the phylogenetic analysis, chemotaxonomic data, physiological characteristics and DNA-DNA hybridization data, strain THG-T121T represents a novel species of the genus Actinotalea, for which the name Actinotaleasolisilvae sp.
29458505	12	62	theme	fermentans	1403:1412	arg1	3251T					1419:1423	A. fermentans KCTC 3251T	1400:1423	A. fermentans KCTC 3251T	1400:1423	DNA-DNA hybridization values between strain THG-T121T and A. ferrariae KCTC 29134T, A. fermentans KCTC 3251T and C. carbonis KCTC 19824T were 30.2 % (27.3 %, reciprocal analysis), 28.4 %, (17.3 %) and 16.9 %, (9.3 %), respectively.
29458505	4	63	theme	rRNA	359:362	arg1	analysis					378:385	16S rRNA gene sequence analysis	355:385	16S rRNA gene sequence analysis	355:385	Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-T121T were identified as Actinotalea ferrariae KCTC 29134T (97.9 %), Actinotalea fermentans KCTC 3251T (97.3 %), Cellulomonas carbonis KCTC 19824T (97.2 %).
29458505	9	64	theme	strain	1119:1124	arg1	THG-T121T					1126:1134	strain THG-T121T	1119:1134	strain THG-T121T	1119:1134	The whole-cell sugars of strain THG-T121T were rhamnose, ribose, mannose and glucose.
29458505	4	65	theme	strain	427:432	arg1	THG-T121T					434:442	strain THG-T121T	427:442	strain THG-T121T	427:442	Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-T121T were identified as Actinotalea ferrariae KCTC 29134T (97.9 %), Actinotalea fermentans KCTC 3251T (97.3 %), Cellulomonas carbonis KCTC 19824T (97.2 %).
29458505	13	66	theme	DNA-DNA	1646:1652	arg1	hybridization					1654:1666	DNA-DNA hybridization	1646:1666	DNA-DNA hybridization	1646:1666	On the basis of the phylogenetic analysis, chemotaxonomic data, physiological characteristics and DNA-DNA hybridization data, strain THG-T121T represents a novel species of the genus Actinotalea, for which the name Actinotaleasolisilvae sp.
29458505	5	67	theme	other	658:662	arg1	species					675:681	other recognized species	658:681	other recognized species	658:681	16S rRNA gene sequence similarities among strain THG-T121T and other recognized species were lower than 97.0 %.
29458505	12	68	theme	KCTC	1387:1390	arg1	29134T					1392:1397	A. ferrariae KCTC 29134T	1374:1397	A. ferrariae KCTC 29134T	1374:1397	DNA-DNA hybridization values between strain THG-T121T and A. ferrariae KCTC 29134T, A. fermentans KCTC 3251T and C. carbonis KCTC 19824T were 30.2 % (27.3 %, reciprocal analysis), 28.4 %, (17.3 %) and 16.9 %, (9.3 %), respectively.
29458505	5	69	theme	sequence	609:616	arg1	similarities					618:629	16S rRNA gene sequence similarities	595:629	16S rRNA gene sequence similarities among strain THG-T121T	595:652	16S rRNA gene sequence similarities among strain THG-T121T and other recognized species were lower than 97.0 %.
29458505	1	70	theme	forest	47:52	arg1	soil					54:57	forest soil	47:57	forest soil	47:57	nov., isolated from forest soil and emended description of the genus Actinotalea.
29458505	2	71	attach	isolated	216:223	arg1	soil					237:240	forest soil	230:240	forest soil	230:240	A Gram-stain-positive, aerobic, non-motile and short-rod-shaped actinobacterium, designated THG-T121T, was isolated from forest soil.
29458505	2	71	attach	isolated	216:223	arg2	actinobacterium					173:187	A Gram-stain-positive, aerobic, non-motile and short-rod-shaped actinobacterium	109:187	A Gram-stain-positive, aerobic, non-motile and short-rod-shaped actinobacterium	109:187	A Gram-stain-positive, aerobic, non-motile and short-rod-shaped actinobacterium, designated THG-T121T, was isolated from forest soil.
29458505	3	72	theme	0-4 	320:323	arg1	%					324:324	%	324:324	%	324:324	Growth occurred at 10-40 °C (optimum 28-30 °C), at pH 6-8 (optimum 7) and at 0-4 % NaCl (optimum 1 %).
29458505	4	73	theme	nearest	392:398	arg1	29134T					490:495	Actinotalea ferrariae KCTC 29134T	463:495	Actinotalea ferrariae KCTC 29134T (97.9 %)	463:504	Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-T121T were identified as Actinotalea ferrariae KCTC 29134T (97.9 %), Actinotalea fermentans KCTC 3251T (97.3 %), Cellulomonas carbonis KCTC 19824T (97.2 %).
29458505	4	73	theme	nearest	392:398	arg1	neighbours					413:422	the nearest phylogenetic neighbours	388:422	the nearest phylogenetic neighbours of strain THG-T121T	388:442	Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-T121T were identified as Actinotalea ferrariae KCTC 29134T (97.9 %), Actinotalea fermentans KCTC 3251T (97.3 %), Cellulomonas carbonis KCTC 19824T (97.2 %).
29458505	11	74	theme	strain	1284:1289	arg1	THG-T121T					1291:1299	strain THG-T121T	1284:1299	strain THG-T121T	1284:1299	The DNA G+C content of strain THG-T121T was 72.4 mol%.
29458505	12	75	theme	reciprocal	1474:1483	arg1	%					1471:1471	27.3 %	1466:1471	27.3 %	1466:1471	DNA-DNA hybridization values between strain THG-T121T and A. ferrariae KCTC 29134T, A. fermentans KCTC 3251T and C. carbonis KCTC 19824T were 30.2 % (27.3 %, reciprocal analysis), 28.4 %, (17.3 %) and 16.9 %, (9.3 %), respectively.
29458505	12	75	theme	reciprocal	1474:1483	arg1	analysis					1485:1492	reciprocal analysis	1474:1492	reciprocal analysis	1474:1492	DNA-DNA hybridization values between strain THG-T121T and A. ferrariae KCTC 29134T, A. fermentans KCTC 3251T and C. carbonis KCTC 19824T were 30.2 % (27.3 %, reciprocal analysis), 28.4 %, (17.3 %) and 16.9 %, (9.3 %), respectively.
29458505	0	76	theme	solisilvae	12:21	arg1	sp					23:24	Actinotalea solisilvae sp	0:24	Actinotalea solisilvae sp.	0:25	Actinotalea solisilvae sp.
29458505	8	77	theme	fatty	986:990	arg1	anteiso-C15 					1003:1014	anteiso-C15 	1003:1014	anteiso-C15 	1003:1014	The major fatty acids were anteiso-C15 : 0, anteiso-C15 : 1 A, C16 : 0, iso-C16 : 0, anteiso-C17 : 0 and iso-C17 : 0.
29458505	8	77	theme	fatty	986:990	arg1	acids					992:996	The major fatty acids	976:996	The major fatty acids	976:996	The major fatty acids were anteiso-C15 : 0, anteiso-C15 : 1 A, C16 : 0, iso-C16 : 0, anteiso-C17 : 0 and iso-C17 : 0.
29458505	8	77	theme	fatty	986:990	arg1	 0					1016:1017	 0	1016:1017	 0	1016:1017	The major fatty acids were anteiso-C15 : 0, anteiso-C15 : 1 A, C16 : 0, iso-C16 : 0, anteiso-C17 : 0 and iso-C17 : 0.
29458505	3	78	theme	optimum	332:338	arg1	%					342:342	optimum 1 %	332:342	optimum 1 %	332:342	Growth occurred at 10-40 °C (optimum 28-30 °C), at pH 6-8 (optimum 7) and at 0-4 % NaCl (optimum 1 %).
29458505	3	78	theme	optimum	332:338	arg1	 NaCl					325:329	0-4 % NaCl	320:329	0-4 % NaCl (optimum 1 %)	320:343	Growth occurred at 10-40 °C (optimum 28-30 °C), at pH 6-8 (optimum 7) and at 0-4 % NaCl (optimum 1 %).
29458505	13	79	theme	chemotaxonomic	1591:1604	arg1	data					1606:1609	chemotaxonomic data	1591:1609	chemotaxonomic data	1591:1609	On the basis of the phylogenetic analysis, chemotaxonomic data, physiological characteristics and DNA-DNA hybridization data, strain THG-T121T represents a novel species of the genus Actinotalea, for which the name Actinotaleasolisilvae sp.
29458505	13	80	theme	physiological	1612:1624	arg1	characteristics					1626:1640	physiological characteristics	1612:1640	physiological characteristics	1612:1640	On the basis of the phylogenetic analysis, chemotaxonomic data, physiological characteristics and DNA-DNA hybridization data, strain THG-T121T represents a novel species of the genus Actinotalea, for which the name Actinotaleasolisilvae sp.
29458505	12	81	theme	strain	1353:1358	arg1	THG-T121T					1360:1368	strain THG-T121T	1353:1368	strain THG-T121T	1353:1368	DNA-DNA hybridization values between strain THG-T121T and A. ferrariae KCTC 29134T, A. fermentans KCTC 3251T and C. carbonis KCTC 19824T were 30.2 % (27.3 %, reciprocal analysis), 28.4 %, (17.3 %) and 16.9 %, (9.3 %), respectively.
29458505	2	82	theme	short-rod-shaped	156:171	arg1	actinobacterium					173:187	A Gram-stain-positive, aerobic, non-motile and short-rod-shaped actinobacterium	109:187	A Gram-stain-positive, aerobic, non-motile and short-rod-shaped actinobacterium	109:187	A Gram-stain-positive, aerobic, non-motile and short-rod-shaped actinobacterium, designated THG-T121T, was isolated from forest soil.
29458505	10	83	theme	peptidoglycan	1184:1196	arg1	A4β					1226:1228	A4β	1226:1228	A4β	1226:1228	The peptidoglycan type of strain THG-T121T is A4β, containing l-Orn-D-Ser-L-Asp.
29458505	10	83	theme	peptidoglycan	1184:1196	arg1	type					1198:1201	The peptidoglycan type	1180:1201	The peptidoglycan type of strain THG-T121T	1180:1221	The peptidoglycan type of strain THG-T121T is A4β, containing l-Orn-D-Ser-L-Asp.
29458505	4	84	theme	ferrariae	475:483	arg1	29134T					490:495	Actinotalea ferrariae KCTC 29134T	463:495	Actinotalea ferrariae KCTC 29134T (97.9 %)	463:504	Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-T121T were identified as Actinotalea ferrariae KCTC 29134T (97.9 %), Actinotalea fermentans KCTC 3251T (97.3 %), Cellulomonas carbonis KCTC 19824T (97.2 %).
29458505	4	84	theme	ferrariae	475:483	arg1	%					503:503	97.9 %	498:503	97.9 %	498:503	Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-T121T were identified as Actinotalea ferrariae KCTC 29134T (97.9 %), Actinotalea fermentans KCTC 3251T (97.3 %), Cellulomonas carbonis KCTC 19824T (97.2 %).
29458505	4	84	theme	ferrariae	475:483	arg1	neighbours					413:422	the nearest phylogenetic neighbours	388:422	the nearest phylogenetic neighbours of strain THG-T121T	388:442	Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-T121T were identified as Actinotalea ferrariae KCTC 29134T (97.9 %), Actinotalea fermentans KCTC 3251T (97.3 %), Cellulomonas carbonis KCTC 19824T (97.2 %).
29458505	4	84	theme	ferrariae	475:483	arg1	3251T					535:539	Actinotalea fermentans KCTC 3251T	507:539	Actinotalea fermentans KCTC 3251T (97.3 %)	507:548	Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-T121T were identified as Actinotalea ferrariae KCTC 29134T (97.9 %), Actinotalea fermentans KCTC 3251T (97.3 %), Cellulomonas carbonis KCTC 19824T (97.2 %).
29458505	4	84	theme	ferrariae	475:483	arg1	19824T					578:583	Cellulomonas carbonis KCTC 19824T	551:583	Cellulomonas carbonis KCTC 19824T (97.2 %)	551:592	Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-T121T were identified as Actinotalea ferrariae KCTC 29134T (97.9 %), Actinotalea fermentans KCTC 3251T (97.3 %), Cellulomonas carbonis KCTC 19824T (97.2 %).
29458505	12	85	theme	DNA-DNA	1316:1322	arg1	hybridization					1324:1336	DNA-DNA hybridization	1316:1336	DNA-DNA hybridization values between strain THG-T121T and A. ferrariae KCTC 29134T, A. fermentans KCTC 3251T and C. carbonis KCTC 19824T	1316:1451	DNA-DNA hybridization values between strain THG-T121T and A. ferrariae KCTC 29134T, A. fermentans KCTC 3251T and C. carbonis KCTC 19824T were 30.2 % (27.3 %, reciprocal analysis), 28.4 %, (17.3 %) and 16.9 %, (9.3 %), respectively.
29458505	4	86	theme	KCTC	530:533	arg1	29134T					490:495	Actinotalea ferrariae KCTC 29134T	463:495	Actinotalea ferrariae KCTC 29134T (97.9 %)	463:504	Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-T121T were identified as Actinotalea ferrariae KCTC 29134T (97.9 %), Actinotalea fermentans KCTC 3251T (97.3 %), Cellulomonas carbonis KCTC 19824T (97.2 %).
29458505	4	86	theme	KCTC	530:533	arg1	3251T					535:539	Actinotalea fermentans KCTC 3251T	507:539	Actinotalea fermentans KCTC 3251T (97.3 %)	507:548	Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-T121T were identified as Actinotalea ferrariae KCTC 29134T (97.9 %), Actinotalea fermentans KCTC 3251T (97.3 %), Cellulomonas carbonis KCTC 19824T (97.2 %).
29458505	4	86	theme	KCTC	530:533	arg1	%					547:547	97.3 %	542:547	97.3 %	542:547	Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-T121T were identified as Actinotalea ferrariae KCTC 29134T (97.9 %), Actinotalea fermentans KCTC 3251T (97.3 %), Cellulomonas carbonis KCTC 19824T (97.2 %).
29458505	10	87	theme	THG-T121T	1213:1221	arg1	A4β					1226:1228	A4β	1226:1228	A4β	1226:1228	The peptidoglycan type of strain THG-T121T is A4β, containing l-Orn-D-Ser-L-Asp.
29458505	10	87	theme	THG-T121T	1213:1221	arg1	type					1198:1201	The peptidoglycan type	1180:1201	The peptidoglycan type of strain THG-T121T	1180:1221	The peptidoglycan type of strain THG-T121T is A4β, containing l-Orn-D-Ser-L-Asp.
29458505	6	88	theme	polar	711:715	arg1	mannosides					822:831	diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two phosphatidylinositol mannosides	729:831	mannosides	822:831	The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two phosphatidylinositol mannosides, one unidentified phospholipid, three unidentified glycolipids and one unidentified lipid.
29458505	6	88	theme	polar	711:715	arg1	lipids					717:722	The polar lipids	707:722	The polar lipids	707:722	The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two phosphatidylinositol mannosides, one unidentified phospholipid, three unidentified glycolipids and one unidentified lipid.
29458505	15	89	theme	=KACC	1837:1841	arg1	4.7389T					1856:1862	=KACC 19191T=CGMCC 4.7389T	1837:1862	=KACC 19191T=CGMCC 4.7389T	1837:1862	The type strain is THG-T121T (=KACC 19191T=CGMCC 4.7389T).
29458505	15	89	theme	=KACC	1837:1841	arg1	THG-T121T					1826:1834	THG-T121T	1826:1834	THG-T121T (=KACC 19191T=CGMCC 4.7389T)	1826:1863	The type strain is THG-T121T (=KACC 19191T=CGMCC 4.7389T).
29458505	6	90	dep	mannosides	822:831	arg1	phosphatidylglycerol					753:772	diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two phosphatidylinositol mannosides	729:831	phosphatidylglycerol	753:772	The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two phosphatidylinositol mannosides, one unidentified phospholipid, three unidentified glycolipids and one unidentified lipid.
29458505	6	90	dep	mannosides	822:831	arg1	phosphatidylinositol					775:794	diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two phosphatidylinositol mannosides	729:831	phosphatidylinositol	775:794	The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two phosphatidylinositol mannosides, one unidentified phospholipid, three unidentified glycolipids and one unidentified lipid.
29458505	2	91	theme	Gram-stain-positive	111:129	arg1	actinobacterium					173:187	A Gram-stain-positive, aerobic, non-motile and short-rod-shaped actinobacterium	109:187	A Gram-stain-positive, aerobic, non-motile and short-rod-shaped actinobacterium	109:187	A Gram-stain-positive, aerobic, non-motile and short-rod-shaped actinobacterium, designated THG-T121T, was isolated from forest soil.
29458505	13	92	theme	name	1758:1761	arg1	sp					1785:1786	the name Actinotaleasolisilvae sp	1754:1786	the name Actinotaleasolisilvae sp	1754:1786	On the basis of the phylogenetic analysis, chemotaxonomic data, physiological characteristics and DNA-DNA hybridization data, strain THG-T121T represents a novel species of the genus Actinotalea, for which the name Actinotaleasolisilvae sp.
29458505	10	93	contain	containing	1231:1240	arg1	A4β					1226:1228	A4β	1226:1228	A4β	1226:1228	The peptidoglycan type of strain THG-T121T is A4β, containing l-Orn-D-Ser-L-Asp.
29458505	10	93	contain	containing	1231:1240	arg1	type					1198:1201	The peptidoglycan type	1180:1201	The peptidoglycan type of strain THG-T121T	1180:1221	The peptidoglycan type of strain THG-T121T is A4β, containing l-Orn-D-Ser-L-Asp.
29458505	10	93	contain	containing	1231:1240	arg2	l-Orn-D-Ser-L-Asp					1242:1258	l-Orn-D-Ser-L-Asp	1242:1258	l-Orn-D-Ser-L-Asp	1242:1258	The peptidoglycan type of strain THG-T121T is A4β, containing l-Orn-D-Ser-L-Asp.
31500064	6	0	theme	marker	1006:1011	arg1	genes					1013:1017	osteogenic marker genes	995:1017	osteogenic marker genes	995:1017	The IONP addition greatly promoted the osteogenesis of hDPSCs, elevating the ALP activity, the expression of osteogenic marker genes and bone matrix formation with 1.5-2-fold increases.
31500064	12	1	theme	stem	1693:1696	arg1	cells					1698:1702	stem cells	1693:1702	stem cells	1693:1702	The novel CPC + IONP composite scaffolds with stem cells are promising to provide an innovative strategy to enhance bone regenerative therapies.
31500064	3	2	theme	%	627:627	arg1	solution					638:645	5% chitosan solution	626:645	5% chitosan solution	626:645	A series of CPC + IONP magnetic scaffolds with different IONP contents (1%, 3% and 6%) were fabricated using 5% chitosan solution as the cement liquid.
31500064	3	2	theme	%	627:627	arg1	cement					654:659	the cement liquid	650:666	the cement liquid	650:666	A series of CPC + IONP magnetic scaffolds with different IONP contents (1%, 3% and 6%) were fabricated using 5% chitosan solution as the cement liquid.
31500064	9	3	theme	hDPSCs	1352:1357	arg1	behavior					1340:1347	the osteogenic behavior	1325:1347	the osteogenic behavior of hDPSCs	1325:1357	The results indicated that the osteogenic behavior of hDPSCs was likely driven by CPC + IONP via the WNT signaling pathway.
31500064	1	4	theme	Calcium	131:137	arg1	phosphate					139:147	Calcium phosphate	131:147	Calcium phosphate cement (CPC)	131:160	Calcium phosphate cement (CPC), functionalized with iron oxide nanoparticles (IONP), is of great promise to promote osteoinduction and new bone formation.
31500064	1	5	theme	iron	183:186	arg1	IONP					209:212	IONP	209:212	IONP	209:212	Calcium phosphate cement (CPC), functionalized with iron oxide nanoparticles (IONP), is of great promise to promote osteoinduction and new bone formation.
31500064	1	5	theme	iron	183:186	arg1	nanoparticles					194:206	iron oxide nanoparticles	183:206	iron oxide nanoparticles (IONP)	183:213	Calcium phosphate cement (CPC), functionalized with iron oxide nanoparticles (IONP), is of great promise to promote osteoinduction and new bone formation.
31500064	12	6	theme	CPC + IONP	1657:1666	arg1	scaffolds					1678:1686	The novel CPC + IONP composite scaffolds	1647:1686	The novel CPC + IONP composite scaffolds with stem cells	1647:1702	The novel CPC + IONP composite scaffolds with stem cells are promising to provide an innovative strategy to enhance bone regenerative therapies.
31500064	12	6	theme	CPC + IONP	1657:1666	arg1	promising					1708:1716	promising	1708:1716	promising	1708:1716	The novel CPC + IONP composite scaffolds with stem cells are promising to provide an innovative strategy to enhance bone regenerative therapies.
31500064	8	7	theme	extracellular	1162:1174	arg1	kinases					1191:1197	the extracellular signal-related kinases	1158:1197	the extracellular signal-related kinases WNT/β-catenin in DPSCs	1158:1220	Activation of the extracellular signal-related kinases WNT/β-catenin in DPSCs was observed, and this activation was attenuated by the WNT inhibitor DKK1.
31500064	8	8	theme	kinases	1191:1197	arg1	Activation					1144:1153	Activation	1144:1153	Activation of the extracellular signal-related kinases WNT/β-catenin in DPSCs	1144:1220	Activation of the extracellular signal-related kinases WNT/β-catenin in DPSCs was observed, and this activation was attenuated by the WNT inhibitor DKK1.
31500064	10	9	theme	osteogenic	1507:1516	arg1	differentiation					1518:1532	osteogenic differentiation	1507:1532	osteogenic differentiation	1507:1532	In conclusion, incorporate IONP into CPC scaffold remarkably enhanced the spreading, osteogenic differentiation and bone mineral synthesis of stem cell.
31500064	10	10	dep	spreading	1496:1504	arg1	the					1492:1494	the	1492:1494	the	1492:1494	In conclusion, incorporate IONP into CPC scaffold remarkably enhanced the spreading, osteogenic differentiation and bone mineral synthesis of stem cell.
31500064	3	11	theme	scaffolds	549:557	arg1	series					519:524	A series	517:524	A series of CPC + IONP magnetic scaffolds with different IONP contents (1%, 3% and 6%)	517:602	A series of CPC + IONP magnetic scaffolds with different IONP contents (1%, 3% and 6%) were fabricated using 5% chitosan solution as the cement liquid.
31500064	9	12	theme	WNT	1399:1401	arg1	pathway					1413:1419	the WNT signaling pathway	1395:1419	the WNT signaling pathway	1395:1419	The results indicated that the osteogenic behavior of hDPSCs was likely driven by CPC + IONP via the WNT signaling pathway.
31500064	10	13	theme	mineral	1543:1549	arg1	synthesis					1551:1559	bone mineral synthesis	1538:1559	bone mineral synthesis	1538:1559	In conclusion, incorporate IONP into CPC scaffold remarkably enhanced the spreading, osteogenic differentiation and bone mineral synthesis of stem cell.
31500064	3	14	theme	liquid	661:666	arg1	solution					638:645	5% chitosan solution	626:645	5% chitosan solution	626:645	A series of CPC + IONP magnetic scaffolds with different IONP contents (1%, 3% and 6%) were fabricated using 5% chitosan solution as the cement liquid.
31500064	3	14	theme	liquid	661:666	arg1	cement					654:659	the cement liquid	650:666	the cement liquid	650:666	A series of CPC + IONP magnetic scaffolds with different IONP contents (1%, 3% and 6%) were fabricated using 5% chitosan solution as the cement liquid.
31500064	3	15	theme	CPC + IONP	529:538	arg1	scaffolds					549:557	CPC + IONP magnetic scaffolds	529:557	CPC + IONP magnetic scaffolds with different IONP contents (1%, 3% and 6%)	529:602	A series of CPC + IONP magnetic scaffolds with different IONP contents (1%, 3% and 6%) were fabricated using 5% chitosan solution as the cement liquid.
31500064	1	16	theme	phosphate	139:147	arg1	cement					149:154	Calcium phosphate cement	131:154	Calcium phosphate cement (CPC)	131:160	Calcium phosphate cement (CPC), functionalized with iron oxide nanoparticles (IONP), is of great promise to promote osteoinduction and new bone formation.
31500064	1	16	theme	phosphate	139:147	arg1	CPC					157:159	CPC	157:159	CPC	157:159	Calcium phosphate cement (CPC), functionalized with iron oxide nanoparticles (IONP), is of great promise to promote osteoinduction and new bone formation.
31500064	12	17	theme	novel	1651:1655	arg1	scaffolds					1678:1686	The novel CPC + IONP composite scaffolds	1647:1686	The novel CPC + IONP composite scaffolds with stem cells	1647:1702	The novel CPC + IONP composite scaffolds with stem cells are promising to provide an innovative strategy to enhance bone regenerative therapies.
31500064	12	17	theme	novel	1651:1655	arg1	promising					1708:1716	promising	1708:1716	promising	1708:1716	The novel CPC + IONP composite scaffolds with stem cells are promising to provide an innovative strategy to enhance bone regenerative therapies.
31500064	6	18	theme	hDPSCs	941:946	arg1	osteogenesis					925:936	the osteogenesis	921:936	the osteogenesis of hDPSCs	921:946	The IONP addition greatly promoted the osteogenesis of hDPSCs, elevating the ALP activity, the expression of osteogenic marker genes and bone matrix formation with 1.5-2-fold increases.
31500064	2	19	theme	human	464:468	arg1	hDPSCs					494:499	hDPSCs	494:499	hDPSCs	494:499	In this work, the IONP powder was added into the CPC powder to fabricate CPC + IONP scaffolds and the effects of the novel composite on bone matrix formation and osteogenesis of human dental pulp stem cells (hDPSCs) were explored.
31500064	2	19	theme	human	464:468	arg1	cells					487:491	human dental pulp stem cells	464:491	human dental pulp stem cells (hDPSCs)	464:500	In this work, the IONP powder was added into the CPC powder to fabricate CPC + IONP scaffolds and the effects of the novel composite on bone matrix formation and osteogenesis of human dental pulp stem cells (hDPSCs) were explored.
31500064	0	20	theme	nanoparticle-calcium	11:30	arg1	cement					42:47	Iron oxide nanoparticle-calcium phosphate cement	0:47	Iron oxide nanoparticle-calcium phosphate cement	0:47	Iron oxide nanoparticle-calcium phosphate cement enhanced the osteogenic activities of stem cells through WNT/β-catenin signaling.
31500064	12	21	with	scaffolds	1678:1686	arg1	cells					1698:1702	stem cells	1693:1702	stem cells	1693:1702	The novel CPC + IONP composite scaffolds with stem cells are promising to provide an innovative strategy to enhance bone regenerative therapies.
31500064	0	22	theme	stem	87:90	arg1	cells					92:96	stem cells	87:96	stem cells	87:96	Iron oxide nanoparticle-calcium phosphate cement enhanced the osteogenic activities of stem cells through WNT/β-catenin signaling.
31500064	2	23	theme	cells	487:491	arg1	osteogenesis					448:459	osteogenesis	448:459	osteogenesis	448:459	In this work, the IONP powder was added into the CPC powder to fabricate CPC + IONP scaffolds and the effects of the novel composite on bone matrix formation and osteogenesis of human dental pulp stem cells (hDPSCs) were explored.
31500064	2	23	theme	cells	487:491	arg1	formation					434:442	bone matrix formation	422:442	bone matrix formation	422:442	In this work, the IONP powder was added into the CPC powder to fabricate CPC + IONP scaffolds and the effects of the novel composite on bone matrix formation and osteogenesis of human dental pulp stem cells (hDPSCs) were explored.
31500064	2	24	theme	pulp	477:480	arg1	hDPSCs					494:499	hDPSCs	494:499	hDPSCs	494:499	In this work, the IONP powder was added into the CPC powder to fabricate CPC + IONP scaffolds and the effects of the novel composite on bone matrix formation and osteogenesis of human dental pulp stem cells (hDPSCs) were explored.
31500064	2	24	theme	pulp	477:480	arg1	cells					487:491	human dental pulp stem cells	464:491	human dental pulp stem cells (hDPSCs)	464:500	In this work, the IONP powder was added into the CPC powder to fabricate CPC + IONP scaffolds and the effects of the novel composite on bone matrix formation and osteogenesis of human dental pulp stem cells (hDPSCs) were explored.
31500064	8	25	theme	WNT	1278:1280	arg1	DKK1					1292:1295	the WNT inhibitor DKK1	1274:1295	the WNT inhibitor DKK1	1274:1295	Activation of the extracellular signal-related kinases WNT/β-catenin in DPSCs was observed, and this activation was attenuated by the WNT inhibitor DKK1.
31500064	2	26	theme	CPC + IONP	359:368	arg1	scaffolds					370:378	CPC + IONP scaffolds	359:378	CPC + IONP scaffolds	359:378	In this work, the IONP powder was added into the CPC powder to fabricate CPC + IONP scaffolds and the effects of the novel composite on bone matrix formation and osteogenesis of human dental pulp stem cells (hDPSCs) were explored.
31500064	3	27	with	scaffolds	549:557	arg1	contents					579:586	different IONP contents	564:586	different IONP contents (1%, 3% and 6%)	564:602	A series of CPC + IONP magnetic scaffolds with different IONP contents (1%, 3% and 6%) were fabricated using 5% chitosan solution as the cement liquid.
31500064	2	28	theme	composite	409:417	arg1	effects					388:394	the effects	384:394	the effects of the novel composite on bone matrix formation and osteogenesis of human dental pulp stem cells (hDPSCs)	384:500	In this work, the IONP powder was added into the CPC powder to fabricate CPC + IONP scaffolds and the effects of the novel composite on bone matrix formation and osteogenesis of human dental pulp stem cells (hDPSCs) were explored.
31500064	3	29	theme	chitosan	629:636	arg1	solution					638:645	5% chitosan solution	626:645	5% chitosan solution	626:645	A series of CPC + IONP magnetic scaffolds with different IONP contents (1%, 3% and 6%) were fabricated using 5% chitosan solution as the cement liquid.
31500064	3	29	theme	chitosan	629:636	arg1	cement					654:659	the cement liquid	650:666	the cement liquid	650:666	A series of CPC + IONP magnetic scaffolds with different IONP contents (1%, 3% and 6%) were fabricated using 5% chitosan solution as the cement liquid.
31500064	0	30	theme	Iron	0:3	arg1	cement					42:47	Iron oxide nanoparticle-calcium phosphate cement	0:47	Iron oxide nanoparticle-calcium phosphate cement	0:47	Iron oxide nanoparticle-calcium phosphate cement enhanced the osteogenic activities of stem cells through WNT/β-catenin signaling.
31500064	1	31	theme	new	266:268	arg1	formation					275:283	new bone formation	266:283	new bone formation	266:283	Calcium phosphate cement (CPC), functionalized with iron oxide nanoparticles (IONP), is of great promise to promote osteoinduction and new bone formation.
31500064	7	32	theme	%	1077:1077	arg1	incorporation					1084:1096	The 3% IONP incorporation	1072:1096	The 3% IONP incorporation	1072:1096	The 3% IONP incorporation showed the most enhancement among all groups.
31500064	5	33	theme	cellular	865:872	arg1	activities					874:883	cellular activities	865:883	cellular activities	865:883	The IONP incorporation substantially enhanced the performance of CPC + IONP, with increases in both mechanical strength and cellular activities.
31500064	6	34	theme	ALP	963:965	arg1	activity					967:974	the ALP activity	959:974	the ALP activity	959:974	The IONP addition greatly promoted the osteogenesis of hDPSCs, elevating the ALP activity, the expression of osteogenic marker genes and bone matrix formation with 1.5-2-fold increases.
31500064	11	35	theme	tissue	1627:1632	arg1	engineering					1634:1644	bone tissue engineering	1622:1644	bone tissue engineering	1622:1644	Therefore, this method had great potential for bone tissue engineering.
31500064	2	36	theme	bone	422:425	arg1	formation					434:442	bone matrix formation	422:442	bone matrix formation	422:442	In this work, the IONP powder was added into the CPC powder to fabricate CPC + IONP scaffolds and the effects of the novel composite on bone matrix formation and osteogenesis of human dental pulp stem cells (hDPSCs) were explored.
31500064	4	37	theme	signaling	722:730	arg1	pathway					732:738	the signaling pathway	718:738	the signaling pathway	718:738	Western blotting and RT-PCR were used to analyze the signaling pathway.
31500064	12	38	theme	regenerative	1768:1779	arg1	therapies					1781:1789	bone regenerative therapies	1763:1789	bone regenerative therapies	1763:1789	The novel CPC + IONP composite scaffolds with stem cells are promising to provide an innovative strategy to enhance bone regenerative therapies.
31500064	12	39	theme	bone	1763:1766	arg1	therapies					1781:1789	bone regenerative therapies	1763:1789	bone regenerative therapies	1763:1789	The novel CPC + IONP composite scaffolds with stem cells are promising to provide an innovative strategy to enhance bone regenerative therapies.
31500064	3	40	theme	different	564:572	arg1	contents					579:586	different IONP contents	564:586	different IONP contents (1%, 3% and 6%)	564:602	A series of CPC + IONP magnetic scaffolds with different IONP contents (1%, 3% and 6%) were fabricated using 5% chitosan solution as the cement liquid.
31500064	2	41	theme	IONP	304:307	arg1	powder					309:314	the IONP powder	300:314	the IONP powder	300:314	In this work, the IONP powder was added into the CPC powder to fabricate CPC + IONP scaffolds and the effects of the novel composite on bone matrix formation and osteogenesis of human dental pulp stem cells (hDPSCs) were explored.
31500064	6	42	theme	bone	1023:1026	arg1	formation					1035:1043	bone matrix formation	1023:1043	bone matrix formation	1023:1043	The IONP addition greatly promoted the osteogenesis of hDPSCs, elevating the ALP activity, the expression of osteogenic marker genes and bone matrix formation with 1.5-2-fold increases.
31500064	2	43	theme	CPC	335:337	arg1	powder					339:344	the CPC powder	331:344	the CPC powder	331:344	In this work, the IONP powder was added into the CPC powder to fabricate CPC + IONP scaffolds and the effects of the novel composite on bone matrix formation and osteogenesis of human dental pulp stem cells (hDPSCs) were explored.
31500064	1	44	theme	great	222:226	arg1	promise					228:234	great promise	222:234	great promise	222:234	Calcium phosphate cement (CPC), functionalized with iron oxide nanoparticles (IONP), is of great promise to promote osteoinduction and new bone formation.
31500064	2	45	from	effects	388:394	arg1	osteogenesis					448:459	osteogenesis	448:459	osteogenesis	448:459	In this work, the IONP powder was added into the CPC powder to fabricate CPC + IONP scaffolds and the effects of the novel composite on bone matrix formation and osteogenesis of human dental pulp stem cells (hDPSCs) were explored.
31500064	2	45	from	effects	388:394	arg1	formation					434:442	bone matrix formation	422:442	bone matrix formation	422:442	In this work, the IONP powder was added into the CPC powder to fabricate CPC + IONP scaffolds and the effects of the novel composite on bone matrix formation and osteogenesis of human dental pulp stem cells (hDPSCs) were explored.
31500064	6	46	theme	genes	1013:1017	arg1	expression					981:990	the expression	977:990	the expression of osteogenic marker genes	977:1017	The IONP addition greatly promoted the osteogenesis of hDPSCs, elevating the ALP activity, the expression of osteogenic marker genes and bone matrix formation with 1.5-2-fold increases.
31500064	6	46	theme	genes	1013:1017	arg1	activity					967:974	the ALP activity	959:974	the ALP activity	959:974	The IONP addition greatly promoted the osteogenesis of hDPSCs, elevating the ALP activity, the expression of osteogenic marker genes and bone matrix formation with 1.5-2-fold increases.
31500064	6	46	theme	genes	1013:1017	arg1	formation					1035:1043	bone matrix formation	1023:1043	bone matrix formation	1023:1043	The IONP addition greatly promoted the osteogenesis of hDPSCs, elevating the ALP activity, the expression of osteogenic marker genes and bone matrix formation with 1.5-2-fold increases.
31500064	10	47	theme	CPC	1459:1461	arg1	scaffold					1463:1470	CPC scaffold	1459:1470	CPC scaffold	1459:1470	In conclusion, incorporate IONP into CPC scaffold remarkably enhanced the spreading, osteogenic differentiation and bone mineral synthesis of stem cell.
31500064	9	48	theme	osteogenic	1329:1338	arg1	behavior					1340:1347	the osteogenic behavior	1325:1347	the osteogenic behavior of hDPSCs	1325:1357	The results indicated that the osteogenic behavior of hDPSCs was likely driven by CPC + IONP via the WNT signaling pathway.
31500064	6	49	theme	osteogenic	995:1004	arg1	genes					1013:1017	osteogenic marker genes	995:1017	osteogenic marker genes	995:1017	The IONP addition greatly promoted the osteogenesis of hDPSCs, elevating the ALP activity, the expression of osteogenic marker genes and bone matrix formation with 1.5-2-fold increases.
31500064	11	50	theme	great	1602:1606	arg1	potential					1608:1616	great potential	1602:1616	great potential for bone tissue engineering	1602:1644	Therefore, this method had great potential for bone tissue engineering.
31500064	12	51	theme	composite	1668:1676	arg1	scaffolds					1678:1686	The novel CPC + IONP composite scaffolds	1647:1686	The novel CPC + IONP composite scaffolds with stem cells	1647:1702	The novel CPC + IONP composite scaffolds with stem cells are promising to provide an innovative strategy to enhance bone regenerative therapies.
31500064	12	51	theme	composite	1668:1676	arg1	promising					1708:1716	promising	1708:1716	promising	1708:1716	The novel CPC + IONP composite scaffolds with stem cells are promising to provide an innovative strategy to enhance bone regenerative therapies.
31500064	3	52	theme	5	626:626	arg1	%					627:627	%	627:627	%	627:627	A series of CPC + IONP magnetic scaffolds with different IONP contents (1%, 3% and 6%) were fabricated using 5% chitosan solution as the cement liquid.
31500064	1	53	theme	oxide	188:192	arg1	IONP					209:212	IONP	209:212	IONP	209:212	Calcium phosphate cement (CPC), functionalized with iron oxide nanoparticles (IONP), is of great promise to promote osteoinduction and new bone formation.
31500064	1	53	theme	oxide	188:192	arg1	nanoparticles					194:206	iron oxide nanoparticles	183:206	iron oxide nanoparticles (IONP)	183:213	Calcium phosphate cement (CPC), functionalized with iron oxide nanoparticles (IONP), is of great promise to promote osteoinduction and new bone formation.
31500064	6	54	theme	IONP	890:893	arg1	addition					895:902	The IONP addition	886:902	The IONP addition	886:902	The IONP addition greatly promoted the osteogenesis of hDPSCs, elevating the ALP activity, the expression of osteogenic marker genes and bone matrix formation with 1.5-2-fold increases.
31500064	4	55	used	used	702:705	arg2	blotting					677:684	Western blotting	669:684	Western blotting	669:684	Western blotting and RT-PCR were used to analyze the signaling pathway.
31500064	4	55	used	used	702:705	arg2	RT-PCR					690:695	RT-PCR	690:695	RT-PCR	690:695	Western blotting and RT-PCR were used to analyze the signaling pathway.
31500064	6	56	theme	1.5-2-fold	1050:1059	arg1	increases					1061:1069	1.5-2-fold increases	1050:1069	1.5-2-fold increases	1050:1069	The IONP addition greatly promoted the osteogenesis of hDPSCs, elevating the ALP activity, the expression of osteogenic marker genes and bone matrix formation with 1.5-2-fold increases.
31500064	8	57	theme	signal-related	1176:1189	arg1	kinases					1191:1197	the extracellular signal-related kinases	1158:1197	the extracellular signal-related kinases WNT/β-catenin in DPSCs	1158:1220	Activation of the extracellular signal-related kinases WNT/β-catenin in DPSCs was observed, and this activation was attenuated by the WNT inhibitor DKK1.
31500064	10	58	theme	stem	1564:1567	arg1	cell					1569:1572	stem cell	1564:1572	stem cell	1564:1572	In conclusion, incorporate IONP into CPC scaffold remarkably enhanced the spreading, osteogenic differentiation and bone mineral synthesis of stem cell.
31500064	3	59	theme	magnetic	540:547	arg1	scaffolds					549:557	CPC + IONP magnetic scaffolds	529:557	CPC + IONP magnetic scaffolds with different IONP contents (1%, 3% and 6%)	529:602	A series of CPC + IONP magnetic scaffolds with different IONP contents (1%, 3% and 6%) were fabricated using 5% chitosan solution as the cement liquid.
31500064	8	60	theme	WNT/β-catenin	1199:1211	arg1	kinases					1191:1197	the extracellular signal-related kinases	1158:1197	the extracellular signal-related kinases WNT/β-catenin in DPSCs	1158:1220	Activation of the extracellular signal-related kinases WNT/β-catenin in DPSCs was observed, and this activation was attenuated by the WNT inhibitor DKK1.
31500064	7	61	theme	most	1109:1112	arg1	enhancement					1114:1124	the most enhancement	1105:1124	the most enhancement among all groups	1105:1141	The 3% IONP incorporation showed the most enhancement among all groups.
31500064	9	62	theme	signaling	1403:1411	arg1	pathway					1413:1419	the WNT signaling pathway	1395:1419	the WNT signaling pathway	1395:1419	The results indicated that the osteogenic behavior of hDPSCs was likely driven by CPC + IONP via the WNT signaling pathway.
31500064	7	63	theme	3	1076:1076	arg1	%					1077:1077	%	1077:1077	%	1077:1077	The 3% IONP incorporation showed the most enhancement among all groups.
31500064	3	64	theme	IONP	574:577	arg1	contents					579:586	different IONP contents	564:586	different IONP contents (1%, 3% and 6%)	564:602	A series of CPC + IONP magnetic scaffolds with different IONP contents (1%, 3% and 6%) were fabricated using 5% chitosan solution as the cement liquid.
31500064	8	65	from	WNT/β-catenin	1199:1211	arg1	DPSCs					1216:1220	DPSCs	1216:1220	DPSCs	1216:1220	Activation of the extracellular signal-related kinases WNT/β-catenin in DPSCs was observed, and this activation was attenuated by the WNT inhibitor DKK1.
31500064	0	66	theme	cells	92:96	arg1	activities					73:82	the osteogenic activities	58:82	the osteogenic activities of stem cells	58:96	Iron oxide nanoparticle-calcium phosphate cement enhanced the osteogenic activities of stem cells through WNT/β-catenin signaling.
31500064	5	67	theme	CPC + IONP	806:815	arg1	performance					791:801	the performance	787:801	the performance of CPC + IONP	787:815	The IONP incorporation substantially enhanced the performance of CPC + IONP, with increases in both mechanical strength and cellular activities.
31500064	0	68	theme	WNT/β-catenin	106:118	arg1	signaling					120:128	WNT/β-catenin signaling	106:128	WNT/β-catenin signaling	106:128	Iron oxide nanoparticle-calcium phosphate cement enhanced the osteogenic activities of stem cells through WNT/β-catenin signaling.
31500064	2	69	theme	stem	482:485	arg1	hDPSCs					494:499	hDPSCs	494:499	hDPSCs	494:499	In this work, the IONP powder was added into the CPC powder to fabricate CPC + IONP scaffolds and the effects of the novel composite on bone matrix formation and osteogenesis of human dental pulp stem cells (hDPSCs) were explored.
31500064	2	69	theme	stem	482:485	arg1	cells					487:491	human dental pulp stem cells	464:491	human dental pulp stem cells (hDPSCs)	464:500	In this work, the IONP powder was added into the CPC powder to fabricate CPC + IONP scaffolds and the effects of the novel composite on bone matrix formation and osteogenesis of human dental pulp stem cells (hDPSCs) were explored.
31500064	8	70	from	DPSCs	1216:1220	arg1	WNT/β-catenin					1199:1211	WNT/β-catenin	1199:1211	WNT/β-catenin	1199:1211	Activation of the extracellular signal-related kinases WNT/β-catenin in DPSCs was observed, and this activation was attenuated by the WNT inhibitor DKK1.
31500064	2	71	theme	dental	470:475	arg1	hDPSCs					494:499	hDPSCs	494:499	hDPSCs	494:499	In this work, the IONP powder was added into the CPC powder to fabricate CPC + IONP scaffolds and the effects of the novel composite on bone matrix formation and osteogenesis of human dental pulp stem cells (hDPSCs) were explored.
31500064	2	71	theme	dental	470:475	arg1	cells					487:491	human dental pulp stem cells	464:491	human dental pulp stem cells (hDPSCs)	464:500	In this work, the IONP powder was added into the CPC powder to fabricate CPC + IONP scaffolds and the effects of the novel composite on bone matrix formation and osteogenesis of human dental pulp stem cells (hDPSCs) were explored.
31500064	4	72	theme	Western	669:675	arg1	blotting					677:684	Western blotting	669:684	Western blotting	669:684	Western blotting and RT-PCR were used to analyze the signaling pathway.
31500064	0	73	theme	oxide	5:9	arg1	cement					42:47	Iron oxide nanoparticle-calcium phosphate cement	0:47	Iron oxide nanoparticle-calcium phosphate cement	0:47	Iron oxide nanoparticle-calcium phosphate cement enhanced the osteogenic activities of stem cells through WNT/β-catenin signaling.
31500064	5	74	theme	mechanical	841:850	arg1	strength					852:859	mechanical strength	841:859	mechanical strength	841:859	The IONP incorporation substantially enhanced the performance of CPC + IONP, with increases in both mechanical strength and cellular activities.
31500064	11	75	contain	had	1598:1600	arg1	method					1591:1596	this method	1586:1596	this method	1586:1596	Therefore, this method had great potential for bone tissue engineering.
31500064	11	75	contain	had	1598:1600	arg2	potential					1608:1616	great potential	1602:1616	great potential for bone tissue engineering	1602:1644	Therefore, this method had great potential for bone tissue engineering.
31500064	2	76	theme	novel	403:407	arg1	composite					409:417	the novel composite	399:417	the novel composite	399:417	In this work, the IONP powder was added into the CPC powder to fabricate CPC + IONP scaffolds and the effects of the novel composite on bone matrix formation and osteogenesis of human dental pulp stem cells (hDPSCs) were explored.
31500064	8	77	theme	inhibitor	1282:1290	arg1	DKK1					1292:1295	the WNT inhibitor DKK1	1274:1295	the WNT inhibitor DKK1	1274:1295	Activation of the extracellular signal-related kinases WNT/β-catenin in DPSCs was observed, and this activation was attenuated by the WNT inhibitor DKK1.
31500064	10	78	theme	cell	1569:1572	arg1	synthesis					1551:1559	bone mineral synthesis	1538:1559	bone mineral synthesis	1538:1559	In conclusion, incorporate IONP into CPC scaffold remarkably enhanced the spreading, osteogenic differentiation and bone mineral synthesis of stem cell.
31500064	10	78	theme	cell	1569:1572	arg1	spreading					1496:1504	spreading	1496:1504	spreading	1496:1504	In conclusion, incorporate IONP into CPC scaffold remarkably enhanced the spreading, osteogenic differentiation and bone mineral synthesis of stem cell.
31500064	10	78	theme	cell	1569:1572	arg1	differentiation					1518:1532	osteogenic differentiation	1507:1532	osteogenic differentiation	1507:1532	In conclusion, incorporate IONP into CPC scaffold remarkably enhanced the spreading, osteogenic differentiation and bone mineral synthesis of stem cell.
31500064	0	79	theme	phosphate	32:40	arg1	cement					42:47	Iron oxide nanoparticle-calcium phosphate cement	0:47	Iron oxide nanoparticle-calcium phosphate cement	0:47	Iron oxide nanoparticle-calcium phosphate cement enhanced the osteogenic activities of stem cells through WNT/β-catenin signaling.
31500064	1	80	theme	bone	270:273	arg1	formation					275:283	new bone formation	266:283	new bone formation	266:283	Calcium phosphate cement (CPC), functionalized with iron oxide nanoparticles (IONP), is of great promise to promote osteoinduction and new bone formation.
31500064	5	81	from	increases	823:831	arg1	strength					852:859	mechanical strength	841:859	mechanical strength	841:859	The IONP incorporation substantially enhanced the performance of CPC + IONP, with increases in both mechanical strength and cellular activities.
31500064	5	81	from	increases	823:831	arg1	activities					874:883	cellular activities	865:883	cellular activities	865:883	The IONP incorporation substantially enhanced the performance of CPC + IONP, with increases in both mechanical strength and cellular activities.
31500064	11	82	theme	bone	1622:1625	arg1	engineering					1634:1644	bone tissue engineering	1622:1644	bone tissue engineering	1622:1644	Therefore, this method had great potential for bone tissue engineering.
31500064	2	83	theme	matrix	427:432	arg1	formation					434:442	bone matrix formation	422:442	bone matrix formation	422:442	In this work, the IONP powder was added into the CPC powder to fabricate CPC + IONP scaffolds and the effects of the novel composite on bone matrix formation and osteogenesis of human dental pulp stem cells (hDPSCs) were explored.
31500064	0	84	theme	osteogenic	62:71	arg1	activities					73:82	the osteogenic activities	58:82	the osteogenic activities of stem cells	58:96	Iron oxide nanoparticle-calcium phosphate cement enhanced the osteogenic activities of stem cells through WNT/β-catenin signaling.
31500064	10	85	theme	incorporate	1437:1447	arg1	IONP					1449:1452	incorporate IONP	1437:1452	incorporate IONP into CPC scaffold	1437:1470	In conclusion, incorporate IONP into CPC scaffold remarkably enhanced the spreading, osteogenic differentiation and bone mineral synthesis of stem cell.
31500064	6	86	theme	matrix	1028:1033	arg1	formation					1035:1043	bone matrix formation	1023:1043	bone matrix formation	1023:1043	The IONP addition greatly promoted the osteogenesis of hDPSCs, elevating the ALP activity, the expression of osteogenic marker genes and bone matrix formation with 1.5-2-fold increases.
31500064	12	87	theme	innovative	1732:1741	arg1	strategy					1743:1750	an innovative strategy	1729:1750	an innovative strategy to enhance bone regenerative therapies	1729:1789	The novel CPC + IONP composite scaffolds with stem cells are promising to provide an innovative strategy to enhance bone regenerative therapies.
31500064	3	88	dep	contents	579:586	arg1	%					590:590	1%	589:590	1%	589:590	A series of CPC + IONP magnetic scaffolds with different IONP contents (1%, 3% and 6%) were fabricated using 5% chitosan solution as the cement liquid.
31500064	3	88	dep	contents	579:586	arg1	%					594:594	3%	593:594	3%	593:594	A series of CPC + IONP magnetic scaffolds with different IONP contents (1%, 3% and 6%) were fabricated using 5% chitosan solution as the cement liquid.
31500064	3	88	dep	contents	579:586	arg1	%					601:601	6%	600:601	6%	600:601	A series of CPC + IONP magnetic scaffolds with different IONP contents (1%, 3% and 6%) were fabricated using 5% chitosan solution as the cement liquid.
31500064	5	89	theme	IONP	745:748	arg1	incorporation					750:762	The IONP incorporation	741:762	The IONP incorporation	741:762	The IONP incorporation substantially enhanced the performance of CPC + IONP, with increases in both mechanical strength and cellular activities.
31500064	7	90	theme	IONP	1079:1082	arg1	incorporation					1084:1096	The 3% IONP incorporation	1072:1096	The 3% IONP incorporation	1072:1096	The 3% IONP incorporation showed the most enhancement among all groups.
31426504	3	0	theme	isotope	532:538	arg1	SILAC					553:557	SILAC	553:557	SILAC	553:557	Scaffolds were repopulated with healthy fibroblasts cultured under static stretch with heavy isotope amino acids (SILAC), to examine newly synthesized proteins over time.
31426504	3	0	theme	isotope	532:538	arg1	acids					546:550	heavy isotope amino acids	526:550	heavy isotope amino acids (SILAC)	526:558	Scaffolds were repopulated with healthy fibroblasts cultured under static stretch with heavy isotope amino acids (SILAC), to examine newly synthesized proteins over time.
31426504	9	1	theme	matrix-directed	1505:1519	arg1	responses					1530:1538	These matrix-directed cellular responses	1499:1538	These matrix-directed cellular responses	1499:1538	These matrix-directed cellular responses emphasize the IPF matrisome and specifically the BM components as important factors for disease progression.
31426504	7	2	theme	proteoglycans	1260:1272	arg1	production					1274:1283	increased periostin and proteoglycans production	1236:1283	increased periostin and proteoglycans production	1236:1283	Fibroblasts produced scaffold-specific proteins mimicking preexisting scaffold composition, where 11 out of 20 BM proteins were differentially expressed, along with increased periostin and proteoglycans production.
31426504	3	3	theme	amino	540:544	arg1	SILAC					553:557	SILAC	553:557	SILAC	553:557	Scaffolds were repopulated with healthy fibroblasts cultured under static stretch with heavy isotope amino acids (SILAC), to examine newly synthesized proteins over time.
31426504	3	3	theme	amino	540:544	arg1	acids					546:550	heavy isotope amino acids	526:550	heavy isotope amino acids (SILAC)	526:558	Scaffolds were repopulated with healthy fibroblasts cultured under static stretch with heavy isotope amino acids (SILAC), to examine newly synthesized proteins over time.
31426504	9	4	theme	BM	1589:1590	arg1	matrisome					1558:1566	the IPF matrisome	1550:1566	the IPF matrisome	1550:1566	These matrix-directed cellular responses emphasize the IPF matrisome and specifically the BM components as important factors for disease progression.
31426504	9	4	theme	BM	1589:1590	arg1	components					1592:1601	the BM components	1585:1601	the BM components	1585:1601	These matrix-directed cellular responses emphasize the IPF matrisome and specifically the BM components as important factors for disease progression.
31426504	9	4	theme	BM	1589:1590	arg1	factors					1616:1622	important factors	1606:1622	important factors for disease progression	1606:1646	These matrix-directed cellular responses emphasize the IPF matrisome and specifically the BM components as important factors for disease progression.
31426504	7	5	theme	increased	1236:1244	arg1	production					1274:1283	increased periostin and proteoglycans production	1236:1283	increased periostin and proteoglycans production	1236:1283	Fibroblasts produced scaffold-specific proteins mimicking preexisting scaffold composition, where 11 out of 20 BM proteins were differentially expressed, along with increased periostin and proteoglycans production.
31426504	2	6	theme	biomechanical	365:377	arg1	properties					379:388	biomechanical properties	365:388	biomechanical properties	365:388	Scaffolds (decellularized tissue) derived from subpleural healthy and IPF lungs were examined regarding biomechanical properties and ECM composition of proteins (the matrisome).
31426504	1	7	theme	matrix	169:174	arg1	properties					137:146	structural properties	126:146	structural properties of the extracellular matrix (ECM)	126:180	In idiopathic pulmonary fibrosis (IPF) structural properties of the extracellular matrix (ECM) are altered and influence cellular responses through cell-matrix interactions.
31426504	1	8	theme	idiopathic	90:99	arg1	IPF					121:123	IPF	121:123	IPF	121:123	In idiopathic pulmonary fibrosis (IPF) structural properties of the extracellular matrix (ECM) are altered and influence cellular responses through cell-matrix interactions.
31426504	1	8	theme	idiopathic	90:99	arg1	fibrosis					111:118	idiopathic pulmonary fibrosis	90:118	idiopathic pulmonary fibrosis (IPF)	90:124	In idiopathic pulmonary fibrosis (IPF) structural properties of the extracellular matrix (ECM) are altered and influence cellular responses through cell-matrix interactions.
31426504	1	9	theme	extracellular	155:167	arg1	ECM					177:179	ECM	177:179	ECM	177:179	In idiopathic pulmonary fibrosis (IPF) structural properties of the extracellular matrix (ECM) are altered and influence cellular responses through cell-matrix interactions.
31426504	1	9	theme	extracellular	155:167	arg1	matrix					169:174	the extracellular matrix	151:174	the extracellular matrix (ECM)	151:180	In idiopathic pulmonary fibrosis (IPF) structural properties of the extracellular matrix (ECM) are altered and influence cellular responses through cell-matrix interactions.
31426504	9	10	theme	important	1606:1614	arg1	matrisome					1558:1566	the IPF matrisome	1550:1566	the IPF matrisome	1550:1566	These matrix-directed cellular responses emphasize the IPF matrisome and specifically the BM components as important factors for disease progression.
31426504	9	10	theme	important	1606:1614	arg1	factors					1616:1622	important factors	1606:1622	important factors for disease progression	1606:1646	These matrix-directed cellular responses emphasize the IPF matrisome and specifically the BM components as important factors for disease progression.
31426504	9	10	theme	important	1606:1614	arg1	components					1592:1601	the BM components	1585:1601	the BM components	1585:1601	These matrix-directed cellular responses emphasize the IPF matrisome and specifically the BM components as important factors for disease progression.
31426504	4	11	theme	differential	703:714	arg1	expressions					716:726	differential expressions	703:726	differential expressions	703:726	IPF scaffolds were characterized by increased tissue density, stiffness, ultimate force, and differential expressions of matrisome proteins compared to healthy scaffolds.
31426504	8	12	theme	novel	1356:1360	arg1	approaches					1362:1371	novel approaches	1356:1371	novel approaches	1356:1371	We demonstrate how matrisome changes affect fibroblast activity using novel approaches to study temporal differences, where IPF scaffolds support a disorganized BM and upregulation of disease-associated proteins.
31426504	7	13	theme	scaffold	1141:1148	arg1	composition					1150:1160	preexisting scaffold composition	1129:1160	preexisting scaffold composition	1129:1160	Fibroblasts produced scaffold-specific proteins mimicking preexisting scaffold composition, where 11 out of 20 BM proteins were differentially expressed, along with increased periostin and proteoglycans production.
31426504	4	14	theme	healthy	762:768	arg1	scaffolds					770:778	healthy scaffolds	762:778	healthy scaffolds	762:778	IPF scaffolds were characterized by increased tissue density, stiffness, ultimate force, and differential expressions of matrisome proteins compared to healthy scaffolds.
31426504	5	15	theme	basement	885:892	arg1	membrane					894:901	basement membrane	885:901	specific basement membrane (BM) proteins such as laminins and collagen IV	876:948	Collagens, proteoglycans, and ECM glycoproteins were increased in IPF scaffolds, however while specific basement membrane (BM) proteins such as laminins and collagen IV were decreased, nidogen-2 was also increased.
31426504	5	15	theme	basement	885:892	arg1	BM					904:905	BM	904:905	BM	904:905	Collagens, proteoglycans, and ECM glycoproteins were increased in IPF scaffolds, however while specific basement membrane (BM) proteins such as laminins and collagen IV were decreased, nidogen-2 was also increased.
31426504	9	16	theme	cellular	1521:1528	arg1	responses					1530:1538	These matrix-directed cellular responses	1499:1538	These matrix-directed cellular responses	1499:1538	These matrix-directed cellular responses emphasize the IPF matrisome and specifically the BM components as important factors for disease progression.
31426504	3	17	with	stretch	513:519	arg1	SILAC					553:557	SILAC	553:557	SILAC	553:557	Scaffolds were repopulated with healthy fibroblasts cultured under static stretch with heavy isotope amino acids (SILAC), to examine newly synthesized proteins over time.
31426504	3	17	with	stretch	513:519	arg1	acids					546:550	heavy isotope amino acids	526:550	heavy isotope amino acids (SILAC)	526:558	Scaffolds were repopulated with healthy fibroblasts cultured under static stretch with heavy isotope amino acids (SILAC), to examine newly synthesized proteins over time.
31426504	2	18	theme	proteins	413:420	arg1	properties					379:388	biomechanical properties	365:388	biomechanical properties	365:388	Scaffolds (decellularized tissue) derived from subpleural healthy and IPF lungs were examined regarding biomechanical properties and ECM composition of proteins (the matrisome).
31426504	2	18	theme	proteins	413:420	arg1	composition					398:408	ECM composition	394:408	ECM composition	394:408	Scaffolds (decellularized tissue) derived from subpleural healthy and IPF lungs were examined regarding biomechanical properties and ECM composition of proteins (the matrisome).
31426504	9	19	theme	disease	1628:1634	arg1	progression					1636:1646	disease progression	1628:1646	disease progression	1628:1646	These matrix-directed cellular responses emphasize the IPF matrisome and specifically the BM components as important factors for disease progression.
31426504	2	20	theme	IPF	331:333	arg1	lungs					335:339	subpleural healthy and IPF lungs	308:339	subpleural healthy and IPF lungs	308:339	Scaffolds (decellularized tissue) derived from subpleural healthy and IPF lungs were examined regarding biomechanical properties and ECM composition of proteins (the matrisome).
31426504	8	21	theme	matrisome	1305:1313	arg1	changes					1315:1321	matrisome changes	1305:1321	matrisome changes	1305:1321	We demonstrate how matrisome changes affect fibroblast activity using novel approaches to study temporal differences, where IPF scaffolds support a disorganized BM and upregulation of disease-associated proteins.
31426504	0	22	theme	Matrisome	0:8	arg1	Properties					10:19	Matrisome Properties	0:19	Matrisome Properties of Scaffolds	0:32	Matrisome Properties of Scaffolds Direct Fibroblasts in Idiopathic Pulmonary Fibrosis.
31426504	7	23	theme	preexisting	1129:1139	arg1	composition					1150:1160	preexisting scaffold composition	1129:1160	preexisting scaffold composition	1129:1160	Fibroblasts produced scaffold-specific proteins mimicking preexisting scaffold composition, where 11 out of 20 BM proteins were differentially expressed, along with increased periostin and proteoglycans production.
31426504	1	24	theme	pulmonary	101:109	arg1	IPF					121:123	IPF	121:123	IPF	121:123	In idiopathic pulmonary fibrosis (IPF) structural properties of the extracellular matrix (ECM) are altered and influence cellular responses through cell-matrix interactions.
31426504	1	24	theme	pulmonary	101:109	arg1	fibrosis					111:118	idiopathic pulmonary fibrosis	90:118	idiopathic pulmonary fibrosis (IPF)	90:124	In idiopathic pulmonary fibrosis (IPF) structural properties of the extracellular matrix (ECM) are altered and influence cellular responses through cell-matrix interactions.
31426504	4	25	theme	ultimate	683:690	arg1	force					692:696	ultimate force	683:696	ultimate force	683:696	IPF scaffolds were characterized by increased tissue density, stiffness, ultimate force, and differential expressions of matrisome proteins compared to healthy scaffolds.
31426504	0	26	theme	Scaffolds	24:32	arg1	Properties					10:19	Matrisome Properties	0:19	Matrisome Properties of Scaffolds	0:32	Matrisome Properties of Scaffolds Direct Fibroblasts in Idiopathic Pulmonary Fibrosis.
31426504	7	27	theme	scaffold-specific	1092:1108	arg1	proteins					1110:1117	scaffold-specific proteins	1092:1117	scaffold-specific proteins mimicking preexisting scaffold composition	1092:1160	Fibroblasts produced scaffold-specific proteins mimicking preexisting scaffold composition, where 11 out of 20 BM proteins were differentially expressed, along with increased periostin and proteoglycans production.
31426504	2	28	theme	healthy	319:325	arg1	lungs					335:339	subpleural healthy and IPF lungs	308:339	subpleural healthy and IPF lungs	308:339	Scaffolds (decellularized tissue) derived from subpleural healthy and IPF lungs were examined regarding biomechanical properties and ECM composition of proteins (the matrisome).
31426504	4	29	theme	tissue	656:661	arg1	density					663:669	increased tissue density	646:669	increased tissue density	646:669	IPF scaffolds were characterized by increased tissue density, stiffness, ultimate force, and differential expressions of matrisome proteins compared to healthy scaffolds.
31426504	8	30	theme	disorganized	1434:1445	arg1	BM					1447:1448	a disorganized BM	1432:1448	a disorganized BM	1432:1448	We demonstrate how matrisome changes affect fibroblast activity using novel approaches to study temporal differences, where IPF scaffolds support a disorganized BM and upregulation of disease-associated proteins.
31426504	2	31	theme	subpleural	308:317	arg1	lungs					335:339	subpleural healthy and IPF lungs	308:339	subpleural healthy and IPF lungs	308:339	Scaffolds (decellularized tissue) derived from subpleural healthy and IPF lungs were examined regarding biomechanical properties and ECM composition of proteins (the matrisome).
31426504	4	32	theme	increased	646:654	arg1	density					663:669	increased tissue density	646:669	increased tissue density	646:669	IPF scaffolds were characterized by increased tissue density, stiffness, ultimate force, and differential expressions of matrisome proteins compared to healthy scaffolds.
31426504	7	33	theme	BM	1182:1183	arg1	proteins					1185:1192	11 out of 20 BM proteins	1169:1192	11 out of 20 BM proteins	1169:1192	Fibroblasts produced scaffold-specific proteins mimicking preexisting scaffold composition, where 11 out of 20 BM proteins were differentially expressed, along with increased periostin and proteoglycans production.
31426504	6	34	theme	disorganized	1054:1065	arg1	BM					1067:1068	a disorganized BM	1052:1068	a disorganized BM	1052:1068	Findings were confirmed with histology, clearly showing a disorganized BM.
31426504	9	35	theme	IPF	1554:1556	arg1	factors					1616:1622	important factors	1606:1622	important factors for disease progression	1606:1646	These matrix-directed cellular responses emphasize the IPF matrisome and specifically the BM components as important factors for disease progression.
31426504	9	35	theme	IPF	1554:1556	arg1	matrisome					1558:1566	the IPF matrisome	1550:1566	the IPF matrisome	1550:1566	These matrix-directed cellular responses emphasize the IPF matrisome and specifically the BM components as important factors for disease progression.
31426504	9	35	theme	IPF	1554:1556	arg1	components					1592:1601	the BM components	1585:1601	the BM components	1585:1601	These matrix-directed cellular responses emphasize the IPF matrisome and specifically the BM components as important factors for disease progression.
31426504	3	36	theme	healthy	471:477	arg1	fibroblasts					479:489	healthy fibroblasts	471:489	healthy fibroblasts cultured under static stretch with heavy isotope amino acids (SILAC)	471:558	Scaffolds were repopulated with healthy fibroblasts cultured under static stretch with heavy isotope amino acids (SILAC), to examine newly synthesized proteins over time.
31426504	5	37	theme	ECM	811:813	arg1	glycoproteins					815:827	ECM glycoproteins	811:827	ECM glycoproteins	811:827	Collagens, proteoglycans, and ECM glycoproteins were increased in IPF scaffolds, however while specific basement membrane (BM) proteins such as laminins and collagen IV were decreased, nidogen-2 was also increased.
31426504	2	38	attach	derived	295:301	arg2	tissue					287:292	decellularized tissue	272:292	decellularized tissue	272:292	Scaffolds (decellularized tissue) derived from subpleural healthy and IPF lungs were examined regarding biomechanical properties and ECM composition of proteins (the matrisome).
31426504	2	38	attach	derived	295:301	arg2	Scaffolds					261:269	Scaffolds	261:269	Scaffolds (decellularized tissue) derived from subpleural healthy and IPF lungs	261:339	Scaffolds (decellularized tissue) derived from subpleural healthy and IPF lungs were examined regarding biomechanical properties and ECM composition of proteins (the matrisome).
31426504	2	38	attach	derived	295:301	arg1	lungs					335:339	subpleural healthy and IPF lungs	308:339	subpleural healthy and IPF lungs	308:339	Scaffolds (decellularized tissue) derived from subpleural healthy and IPF lungs were examined regarding biomechanical properties and ECM composition of proteins (the matrisome).
31426504	5	39	theme	IPF	847:849	arg1	scaffolds					851:859	IPF scaffolds	847:859	IPF scaffolds	847:859	Collagens, proteoglycans, and ECM glycoproteins were increased in IPF scaffolds, however while specific basement membrane (BM) proteins such as laminins and collagen IV were decreased, nidogen-2 was also increased.
31426504	1	40	theme	cellular	208:215	arg1	responses					217:225	cellular responses	208:225	cellular responses	208:225	In idiopathic pulmonary fibrosis (IPF) structural properties of the extracellular matrix (ECM) are altered and influence cellular responses through cell-matrix interactions.
31426504	0	41	theme	Idiopathic	56:65	arg1	Fibrosis					77:84	Idiopathic Pulmonary Fibrosis	56:84	Idiopathic Pulmonary Fibrosis	56:84	Matrisome Properties of Scaffolds Direct Fibroblasts in Idiopathic Pulmonary Fibrosis.
31426504	8	42	theme	proteins	1489:1496	arg1	upregulation					1454:1465	upregulation	1454:1465	upregulation of disease-associated proteins	1454:1496	We demonstrate how matrisome changes affect fibroblast activity using novel approaches to study temporal differences, where IPF scaffolds support a disorganized BM and upregulation of disease-associated proteins.
31426504	8	42	theme	proteins	1489:1496	arg1	BM					1447:1448	a disorganized BM	1432:1448	a disorganized BM	1432:1448	We demonstrate how matrisome changes affect fibroblast activity using novel approaches to study temporal differences, where IPF scaffolds support a disorganized BM and upregulation of disease-associated proteins.
31426504	4	43	theme	matrisome	731:739	arg1	proteins					741:748	matrisome proteins	731:748	matrisome proteins	731:748	IPF scaffolds were characterized by increased tissue density, stiffness, ultimate force, and differential expressions of matrisome proteins compared to healthy scaffolds.
31426504	4	44	theme	IPF	610:612	arg1	scaffolds					614:622	IPF scaffolds	610:622	IPF scaffolds	610:622	IPF scaffolds were characterized by increased tissue density, stiffness, ultimate force, and differential expressions of matrisome proteins compared to healthy scaffolds.
31426504	8	45	theme	disease-associated	1470:1487	arg1	proteins					1489:1496	disease-associated proteins	1470:1496	disease-associated proteins	1470:1496	We demonstrate how matrisome changes affect fibroblast activity using novel approaches to study temporal differences, where IPF scaffolds support a disorganized BM and upregulation of disease-associated proteins.
31426504	3	46	theme	heavy	526:530	arg1	SILAC					553:557	SILAC	553:557	SILAC	553:557	Scaffolds were repopulated with healthy fibroblasts cultured under static stretch with heavy isotope amino acids (SILAC), to examine newly synthesized proteins over time.
31426504	3	46	theme	heavy	526:530	arg1	acids					546:550	heavy isotope amino acids	526:550	heavy isotope amino acids (SILAC)	526:558	Scaffolds were repopulated with healthy fibroblasts cultured under static stretch with heavy isotope amino acids (SILAC), to examine newly synthesized proteins over time.
31426504	3	47	theme	synthesized	578:588	arg1	proteins					590:597	newly synthesized proteins	572:597	newly synthesized proteins	572:597	Scaffolds were repopulated with healthy fibroblasts cultured under static stretch with heavy isotope amino acids (SILAC), to examine newly synthesized proteins over time.
31426504	2	48	theme	decellularized	272:285	arg1	Scaffolds					261:269	Scaffolds	261:269	Scaffolds (decellularized tissue) derived from subpleural healthy and IPF lungs	261:339	Scaffolds (decellularized tissue) derived from subpleural healthy and IPF lungs were examined regarding biomechanical properties and ECM composition of proteins (the matrisome).
31426504	2	48	theme	decellularized	272:285	arg1	tissue					287:292	decellularized tissue	272:292	decellularized tissue	272:292	Scaffolds (decellularized tissue) derived from subpleural healthy and IPF lungs were examined regarding biomechanical properties and ECM composition of proteins (the matrisome).
31426504	1	49	theme	structural	126:135	arg1	properties					137:146	structural properties	126:146	structural properties of the extracellular matrix (ECM)	126:180	In idiopathic pulmonary fibrosis (IPF) structural properties of the extracellular matrix (ECM) are altered and influence cellular responses through cell-matrix interactions.
31426504	8	50	theme	fibroblast	1330:1339	arg1	activity					1341:1348	fibroblast activity	1330:1348	fibroblast activity	1330:1348	We demonstrate how matrisome changes affect fibroblast activity using novel approaches to study temporal differences, where IPF scaffolds support a disorganized BM and upregulation of disease-associated proteins.
31426504	8	51	theme	temporal	1382:1389	arg1	differences					1391:1401	temporal differences	1382:1401	temporal differences	1382:1401	We demonstrate how matrisome changes affect fibroblast activity using novel approaches to study temporal differences, where IPF scaffolds support a disorganized BM and upregulation of disease-associated proteins.
31426504	4	52	theme	proteins	741:748	arg1	density					663:669	increased tissue density	646:669	increased tissue density	646:669	IPF scaffolds were characterized by increased tissue density, stiffness, ultimate force, and differential expressions of matrisome proteins compared to healthy scaffolds.
31426504	4	52	theme	proteins	741:748	arg1	expressions					716:726	differential expressions	703:726	differential expressions	703:726	IPF scaffolds were characterized by increased tissue density, stiffness, ultimate force, and differential expressions of matrisome proteins compared to healthy scaffolds.
31426504	4	52	theme	proteins	741:748	arg1	stiffness					672:680	stiffness	672:680	stiffness	672:680	IPF scaffolds were characterized by increased tissue density, stiffness, ultimate force, and differential expressions of matrisome proteins compared to healthy scaffolds.
31426504	4	52	theme	proteins	741:748	arg1	force					692:696	ultimate force	683:696	ultimate force	683:696	IPF scaffolds were characterized by increased tissue density, stiffness, ultimate force, and differential expressions of matrisome proteins compared to healthy scaffolds.
31426504	3	53	theme	static	506:511	arg1	stretch					513:519	static stretch	506:519	static stretch with heavy isotope amino acids (SILAC)	506:558	Scaffolds were repopulated with healthy fibroblasts cultured under static stretch with heavy isotope amino acids (SILAC), to examine newly synthesized proteins over time.
31426504	5	54	theme	specific	876:883	arg1	collagen					938:945	collagen IV	938:948	collagen IV	938:948	Collagens, proteoglycans, and ECM glycoproteins were increased in IPF scaffolds, however while specific basement membrane (BM) proteins such as laminins and collagen IV were decreased, nidogen-2 was also increased.
31426504	5	54	theme	specific	876:883	arg1	proteins					908:915	specific basement membrane (BM) proteins	876:915	specific basement membrane (BM) proteins such as laminins and collagen IV	876:948	Collagens, proteoglycans, and ECM glycoproteins were increased in IPF scaffolds, however while specific basement membrane (BM) proteins such as laminins and collagen IV were decreased, nidogen-2 was also increased.
31426504	5	54	theme	specific	876:883	arg1	laminins					925:932	laminins	925:932	laminins	925:932	Collagens, proteoglycans, and ECM glycoproteins were increased in IPF scaffolds, however while specific basement membrane (BM) proteins such as laminins and collagen IV were decreased, nidogen-2 was also increased.
31426504	2	55	theme	ECM	394:396	arg1	composition					398:408	ECM composition	394:408	ECM composition	394:408	Scaffolds (decellularized tissue) derived from subpleural healthy and IPF lungs were examined regarding biomechanical properties and ECM composition of proteins (the matrisome).
31426504	5	56	theme	membrane	894:901	arg1	collagen					938:945	collagen IV	938:948	collagen IV	938:948	Collagens, proteoglycans, and ECM glycoproteins were increased in IPF scaffolds, however while specific basement membrane (BM) proteins such as laminins and collagen IV were decreased, nidogen-2 was also increased.
31426504	5	56	theme	membrane	894:901	arg1	proteins					908:915	specific basement membrane (BM) proteins	876:915	specific basement membrane (BM) proteins such as laminins and collagen IV	876:948	Collagens, proteoglycans, and ECM glycoproteins were increased in IPF scaffolds, however while specific basement membrane (BM) proteins such as laminins and collagen IV were decreased, nidogen-2 was also increased.
31426504	5	56	theme	membrane	894:901	arg1	laminins					925:932	laminins	925:932	laminins	925:932	Collagens, proteoglycans, and ECM glycoproteins were increased in IPF scaffolds, however while specific basement membrane (BM) proteins such as laminins and collagen IV were decreased, nidogen-2 was also increased.
31426504	1	57	theme	cell-matrix	235:245	arg1	interactions					247:258	cell-matrix interactions	235:258	cell-matrix interactions	235:258	In idiopathic pulmonary fibrosis (IPF) structural properties of the extracellular matrix (ECM) are altered and influence cellular responses through cell-matrix interactions.
31426504	0	58	theme	Pulmonary	67:75	arg1	Fibrosis					77:84	Idiopathic Pulmonary Fibrosis	56:84	Idiopathic Pulmonary Fibrosis	56:84	Matrisome Properties of Scaffolds Direct Fibroblasts in Idiopathic Pulmonary Fibrosis.
31426504	5	59	gly	glycoproteins	815:827	arg1	glycoproteins					815:827	ECM glycoproteins	811:827	ECM glycoproteins	811:827	Collagens, proteoglycans, and ECM glycoproteins were increased in IPF scaffolds, however while specific basement membrane (BM) proteins such as laminins and collagen IV were decreased, nidogen-2 was also increased.
31426504	7	60	theme	periostin	1246:1254	arg1	production					1274:1283	increased periostin and proteoglycans production	1236:1283	increased periostin and proteoglycans production	1236:1283	Fibroblasts produced scaffold-specific proteins mimicking preexisting scaffold composition, where 11 out of 20 BM proteins were differentially expressed, along with increased periostin and proteoglycans production.
31719620	6	0	theme	structural	1372:1381	arg1	features					1383:1390	the structural features	1368:1390	the structural features identified by these antibodies as revealed in the present study	1368:1454	Because the clinical significance of some of these antibodies was already established, the structural features identified by these antibodies as revealed in the present study should provide useful information relevant to their further clinical use and the biological understanding of MUC1.
31719620	6	1	theme	MUC1	1565:1568	arg1	understanding					1548:1560	the biological understanding	1533:1560	the biological understanding of MUC1	1533:1568	Because the clinical significance of some of these antibodies was already established, the structural features identified by these antibodies as revealed in the present study should provide useful information relevant to their further clinical use and the biological understanding of MUC1.
31719620	6	1	theme	MUC1	1565:1568	arg1	information					1478:1488	useful information	1471:1488	useful information relevant to their further clinical use	1471:1527	Because the clinical significance of some of these antibodies was already established, the structural features identified by these antibodies as revealed in the present study should provide useful information relevant to their further clinical use and the biological understanding of MUC1.
31719620	1	2	theme	Anti-mucin1	159:169	arg1	antibodies					178:187	Anti-mucin1 (MUC1) antibodies	159:187	Anti-mucin1 (MUC1) antibodies	159:187	Anti-mucin1 (MUC1) antibodies have long been used clinically in cancer diagnosis and therapy and specific bindings of some of them are known to be dependent on the differential glycosylation of MUC1.
31719620	3	3	theme	enzymatic	820:828	arg1	extension					830:838	enzymatic extension	820:838	enzymatic extension of carbohydrate chains	820:861	Here, a total of 20 glycopeptides including the tandem repeat unit of MUC1, APPAHGVTSAPDTRPAPGSTAPPAHGV with GalNAc (Tn-antigen), Galβ1-3GalNAc (T-antigen), NeuAcα2-3Galβ1-3GalNAc (sialyl-T-antigen), or NeuAcα2-6GalNAc (sialyl-Tn-antigen) at each threonine or serine residue were prepared by a combination of chemical glycopeptide synthesis and enzymatic extension of carbohydrate chains.
31719620	3	4	with	Galβ1-3GalNAc	605:617	arg1	GalNAc					584:589	GalNAc	584:589	GalNAc (Tn-antigen)	584:602	Here, a total of 20 glycopeptides including the tandem repeat unit of MUC1, APPAHGVTSAPDTRPAPGSTAPPAHGV with GalNAc (Tn-antigen), Galβ1-3GalNAc (T-antigen), NeuAcα2-3Galβ1-3GalNAc (sialyl-T-antigen), or NeuAcα2-6GalNAc (sialyl-Tn-antigen) at each threonine or serine residue were prepared by a combination of chemical glycopeptide synthesis and enzymatic extension of carbohydrate chains.
31719620	3	4	with	Galβ1-3GalNAc	605:617	arg1	Tn-antigen					592:601	Tn-antigen	592:601	Tn-antigen	592:601	Here, a total of 20 glycopeptides including the tandem repeat unit of MUC1, APPAHGVTSAPDTRPAPGSTAPPAHGV with GalNAc (Tn-antigen), Galβ1-3GalNAc (T-antigen), NeuAcα2-3Galβ1-3GalNAc (sialyl-T-antigen), or NeuAcα2-6GalNAc (sialyl-Tn-antigen) at each threonine or serine residue were prepared by a combination of chemical glycopeptide synthesis and enzymatic extension of carbohydrate chains.
31719620	5	5	contain	having	1225:1230	arg1	cases					1219:1223	some cases	1214:1223	some cases having a specific glycan at unique glycosylation sites	1214:1278	The results indicated that anti-MUC1 mAbs have diverse specificities but can be classified into a few characteristic groups based on their binding pattern toward glycopeptides in some cases having a specific glycan at unique glycosylation sites.
31719620	5	5	contain	having	1225:1230	arg2	glycan					1243:1248	a specific glycan	1232:1248	a specific glycan	1232:1248	The results indicated that anti-MUC1 mAbs have diverse specificities but can be classified into a few characteristic groups based on their binding pattern toward glycopeptides in some cases having a specific glycan at unique glycosylation sites.
31719620	3	6	from	total	483:487	arg1	residue					742:748	each threonine or serine residue	717:748	residue	742:748	Here, a total of 20 glycopeptides including the tandem repeat unit of MUC1, APPAHGVTSAPDTRPAPGSTAPPAHGV with GalNAc (Tn-antigen), Galβ1-3GalNAc (T-antigen), NeuAcα2-3Galβ1-3GalNAc (sialyl-T-antigen), or NeuAcα2-6GalNAc (sialyl-Tn-antigen) at each threonine or serine residue were prepared by a combination of chemical glycopeptide synthesis and enzymatic extension of carbohydrate chains.
31719620	3	7	with	NeuAcα2-6GalNAc	678:692	arg1	GalNAc					584:589	GalNAc	584:589	GalNAc (Tn-antigen)	584:602	Here, a total of 20 glycopeptides including the tandem repeat unit of MUC1, APPAHGVTSAPDTRPAPGSTAPPAHGV with GalNAc (Tn-antigen), Galβ1-3GalNAc (T-antigen), NeuAcα2-3Galβ1-3GalNAc (sialyl-T-antigen), or NeuAcα2-6GalNAc (sialyl-Tn-antigen) at each threonine or serine residue were prepared by a combination of chemical glycopeptide synthesis and enzymatic extension of carbohydrate chains.
31719620	3	7	with	NeuAcα2-6GalNAc	678:692	arg1	Tn-antigen					592:601	Tn-antigen	592:601	Tn-antigen	592:601	Here, a total of 20 glycopeptides including the tandem repeat unit of MUC1, APPAHGVTSAPDTRPAPGSTAPPAHGV with GalNAc (Tn-antigen), Galβ1-3GalNAc (T-antigen), NeuAcα2-3Galβ1-3GalNAc (sialyl-T-antigen), or NeuAcα2-6GalNAc (sialyl-Tn-antigen) at each threonine or serine residue were prepared by a combination of chemical glycopeptide synthesis and enzymatic extension of carbohydrate chains.
31719620	3	8	theme	extension	830:838	arg1	combination					769:779	a combination	767:779	a combination of chemical glycopeptide synthesis and enzymatic extension of carbohydrate chains	767:861	Here, a total of 20 glycopeptides including the tandem repeat unit of MUC1, APPAHGVTSAPDTRPAPGSTAPPAHGV with GalNAc (Tn-antigen), Galβ1-3GalNAc (T-antigen), NeuAcα2-3Galβ1-3GalNAc (sialyl-T-antigen), or NeuAcα2-6GalNAc (sialyl-Tn-antigen) at each threonine or serine residue were prepared by a combination of chemical glycopeptide synthesis and enzymatic extension of carbohydrate chains.
31719620	3	9	with	APPAHGVTSAPDTRPAPGSTAPPAHGV	551:577	arg1	GalNAc					584:589	GalNAc	584:589	GalNAc (Tn-antigen)	584:602	Here, a total of 20 glycopeptides including the tandem repeat unit of MUC1, APPAHGVTSAPDTRPAPGSTAPPAHGV with GalNAc (Tn-antigen), Galβ1-3GalNAc (T-antigen), NeuAcα2-3Galβ1-3GalNAc (sialyl-T-antigen), or NeuAcα2-6GalNAc (sialyl-Tn-antigen) at each threonine or serine residue were prepared by a combination of chemical glycopeptide synthesis and enzymatic extension of carbohydrate chains.
31719620	3	9	with	APPAHGVTSAPDTRPAPGSTAPPAHGV	551:577	arg1	Tn-antigen					592:601	Tn-antigen	592:601	Tn-antigen	592:601	Here, a total of 20 glycopeptides including the tandem repeat unit of MUC1, APPAHGVTSAPDTRPAPGSTAPPAHGV with GalNAc (Tn-antigen), Galβ1-3GalNAc (T-antigen), NeuAcα2-3Galβ1-3GalNAc (sialyl-T-antigen), or NeuAcα2-6GalNAc (sialyl-Tn-antigen) at each threonine or serine residue were prepared by a combination of chemical glycopeptide synthesis and enzymatic extension of carbohydrate chains.
31719620	1	10	theme	differential	323:334	arg1	glycosylation					336:348	the differential glycosylation	319:348	the differential glycosylation of MUC1	319:356	Anti-mucin1 (MUC1) antibodies have long been used clinically in cancer diagnosis and therapy and specific bindings of some of them are known to be dependent on the differential glycosylation of MUC1.
31719620	3	11	gly	glycopeptides	495:507	arg2	Galβ1-3GalNAc					605:617	Galβ1-3GalNAc	605:617	Galβ1-3GalNAc (T-antigen)	605:629	Here, a total of 20 glycopeptides including the tandem repeat unit of MUC1, APPAHGVTSAPDTRPAPGSTAPPAHGV with GalNAc (Tn-antigen), Galβ1-3GalNAc (T-antigen), NeuAcα2-3Galβ1-3GalNAc (sialyl-T-antigen), or NeuAcα2-6GalNAc (sialyl-Tn-antigen) at each threonine or serine residue were prepared by a combination of chemical glycopeptide synthesis and enzymatic extension of carbohydrate chains.
31719620	3	11	gly	glycopeptides	495:507	arg2	NeuAcα2-3Galβ1-3GalNAc					632:653	NeuAcα2-3Galβ1-3GalNAc	632:653	NeuAcα2-3Galβ1-3GalNAc (sialyl-T-antigen)	632:672	Here, a total of 20 glycopeptides including the tandem repeat unit of MUC1, APPAHGVTSAPDTRPAPGSTAPPAHGV with GalNAc (Tn-antigen), Galβ1-3GalNAc (T-antigen), NeuAcα2-3Galβ1-3GalNAc (sialyl-T-antigen), or NeuAcα2-6GalNAc (sialyl-Tn-antigen) at each threonine or serine residue were prepared by a combination of chemical glycopeptide synthesis and enzymatic extension of carbohydrate chains.
31719620	3	11	gly	glycopeptides	495:507	arg2	NeuAcα2-6GalNAc					678:692	NeuAcα2-6GalNAc	678:692	NeuAcα2-6GalNAc (sialyl-Tn-antigen)	678:712	Here, a total of 20 glycopeptides including the tandem repeat unit of MUC1, APPAHGVTSAPDTRPAPGSTAPPAHGV with GalNAc (Tn-antigen), Galβ1-3GalNAc (T-antigen), NeuAcα2-3Galβ1-3GalNAc (sialyl-T-antigen), or NeuAcα2-6GalNAc (sialyl-Tn-antigen) at each threonine or serine residue were prepared by a combination of chemical glycopeptide synthesis and enzymatic extension of carbohydrate chains.
31719620	3	11	gly	glycopeptides	495:507	arg2	glycopeptides					495:507	20 glycopeptides	492:507	20 glycopeptides including the tandem repeat unit of MUC1, APPAHGVTSAPDTRPAPGSTAPPAHGV with GalNAc (Tn-antigen), Galβ1-3GalNAc (T-antigen), NeuAcα2-3Galβ1-3GalNAc (sialyl-T-antigen), or NeuAcα2-6GalNAc (sialyl-Tn-antigen) at each threonine or serine residue	492:748	Here, a total of 20 glycopeptides including the tandem repeat unit of MUC1, APPAHGVTSAPDTRPAPGSTAPPAHGV with GalNAc (Tn-antigen), Galβ1-3GalNAc (T-antigen), NeuAcα2-3Galβ1-3GalNAc (sialyl-T-antigen), or NeuAcα2-6GalNAc (sialyl-Tn-antigen) at each threonine or serine residue were prepared by a combination of chemical glycopeptide synthesis and enzymatic extension of carbohydrate chains.
31719620	3	11	gly	glycopeptides	495:507	arg2	APPAHGVTSAPDTRPAPGSTAPPAHGV					551:577	APPAHGVTSAPDTRPAPGSTAPPAHGV	551:577	APPAHGVTSAPDTRPAPGSTAPPAHGV with GalNAc (Tn-antigen)	551:602	Here, a total of 20 glycopeptides including the tandem repeat unit of MUC1, APPAHGVTSAPDTRPAPGSTAPPAHGV with GalNAc (Tn-antigen), Galβ1-3GalNAc (T-antigen), NeuAcα2-3Galβ1-3GalNAc (sialyl-T-antigen), or NeuAcα2-6GalNAc (sialyl-Tn-antigen) at each threonine or serine residue were prepared by a combination of chemical glycopeptide synthesis and enzymatic extension of carbohydrate chains.
31719620	3	11	gly	glycopeptides	495:507	arg2	unit					537:540	the tandem repeat unit	519:540	the tandem repeat unit of MUC1	519:548	Here, a total of 20 glycopeptides including the tandem repeat unit of MUC1, APPAHGVTSAPDTRPAPGSTAPPAHGV with GalNAc (Tn-antigen), Galβ1-3GalNAc (T-antigen), NeuAcα2-3Galβ1-3GalNAc (sialyl-T-antigen), or NeuAcα2-6GalNAc (sialyl-Tn-antigen) at each threonine or serine residue were prepared by a combination of chemical glycopeptide synthesis and enzymatic extension of carbohydrate chains.
31719620	5	12	theme	unique	1253:1258	arg1	sites					1274:1278	unique glycosylation sites	1253:1278	unique glycosylation sites	1253:1278	The results indicated that anti-MUC1 mAbs have diverse specificities but can be classified into a few characteristic groups based on their binding pattern toward glycopeptides in some cases having a specific glycan at unique glycosylation sites.
31719620	1	13	theme	specific	256:263	arg1	bindings					265:272	specific bindings	256:272	specific bindings of some of them	256:288	Anti-mucin1 (MUC1) antibodies have long been used clinically in cancer diagnosis and therapy and specific bindings of some of them are known to be dependent on the differential glycosylation of MUC1.
31719620	4	14	link	enzyme-linked	903:915	arg1	ELISA					938:942	ELISA	938:942	ELISA	938:942	These glycopeptides were tested by the enzyme-linked immunosorbent assay (ELISA) for their capacity to bind 13 monoclonal antibodies (mAbs) known to be specific for MUC1.
31719620	4	14	link	enzyme-linked	903:915	arg1	assay					931:935	the enzyme-linked immunosorbent assay	899:935	the enzyme-linked immunosorbent assay (ELISA) for their capacity to bind 13 monoclonal antibodies (mAbs) known to be specific for MUC1	899:1032	These glycopeptides were tested by the enzyme-linked immunosorbent assay (ELISA) for their capacity to bind 13 monoclonal antibodies (mAbs) known to be specific for MUC1.
31719620	3	15	theme	repeat	530:535	arg1	unit					537:540	the tandem repeat unit	519:540	the tandem repeat unit of MUC1	519:548	Here, a total of 20 glycopeptides including the tandem repeat unit of MUC1, APPAHGVTSAPDTRPAPGSTAPPAHGV with GalNAc (Tn-antigen), Galβ1-3GalNAc (T-antigen), NeuAcα2-3Galβ1-3GalNAc (sialyl-T-antigen), or NeuAcα2-6GalNAc (sialyl-Tn-antigen) at each threonine or serine residue were prepared by a combination of chemical glycopeptide synthesis and enzymatic extension of carbohydrate chains.
31719620	3	16	with	NeuAcα2-3Galβ1-3GalNAc	632:653	arg1	GalNAc					584:589	GalNAc	584:589	GalNAc (Tn-antigen)	584:602	Here, a total of 20 glycopeptides including the tandem repeat unit of MUC1, APPAHGVTSAPDTRPAPGSTAPPAHGV with GalNAc (Tn-antigen), Galβ1-3GalNAc (T-antigen), NeuAcα2-3Galβ1-3GalNAc (sialyl-T-antigen), or NeuAcα2-6GalNAc (sialyl-Tn-antigen) at each threonine or serine residue were prepared by a combination of chemical glycopeptide synthesis and enzymatic extension of carbohydrate chains.
31719620	3	16	with	NeuAcα2-3Galβ1-3GalNAc	632:653	arg1	Tn-antigen					592:601	Tn-antigen	592:601	Tn-antigen	592:601	Here, a total of 20 glycopeptides including the tandem repeat unit of MUC1, APPAHGVTSAPDTRPAPGSTAPPAHGV with GalNAc (Tn-antigen), Galβ1-3GalNAc (T-antigen), NeuAcα2-3Galβ1-3GalNAc (sialyl-T-antigen), or NeuAcα2-6GalNAc (sialyl-Tn-antigen) at each threonine or serine residue were prepared by a combination of chemical glycopeptide synthesis and enzymatic extension of carbohydrate chains.
31719620	5	17	theme	diverse	1082:1088	arg1	specificities					1090:1102	diverse specificities	1082:1102	diverse specificities	1082:1102	The results indicated that anti-MUC1 mAbs have diverse specificities but can be classified into a few characteristic groups based on their binding pattern toward glycopeptides in some cases having a specific glycan at unique glycosylation sites.
31719620	0	18	with	Unit	69:72	arg1	T-					79:80	T-	79:80	T-	79:80	Products of Chemoenzymatic Synthesis Representing MUC1 Tandem Repeat Unit with T-, ST- or STn-antigen Revealed Distinct Specificities of Anti-MUC1 Antibodies.
31719620	0	18	with	Unit	69:72	arg1	STn-antigen					90:100	STn-antigen	90:100	STn-antigen	90:100	Products of Chemoenzymatic Synthesis Representing MUC1 Tandem Repeat Unit with T-, ST- or STn-antigen Revealed Distinct Specificities of Anti-MUC1 Antibodies.
31719620	0	18	with	Unit	69:72	arg1	ST-					83:85	ST-	83:85	ST-	83:85	Products of Chemoenzymatic Synthesis Representing MUC1 Tandem Repeat Unit with T-, ST- or STn-antigen Revealed Distinct Specificities of Anti-MUC1 Antibodies.
31719620	2	19	theme	systematic	370:379	arg1	comparison					381:390	a systematic comparison	368:390	a systematic comparison of the binding specificities of anti-MUC1 antibodies	368:443	However, a systematic comparison of the binding specificities of anti-MUC1 antibodies was not previously conducted.
31719620	5	20	contain	have	1077:1080	arg2	specificities					1090:1102	diverse specificities	1082:1102	diverse specificities	1082:1102	The results indicated that anti-MUC1 mAbs have diverse specificities but can be classified into a few characteristic groups based on their binding pattern toward glycopeptides in some cases having a specific glycan at unique glycosylation sites.
31719620	5	20	contain	have	1077:1080	arg1	mAbs					1072:1075	anti-MUC1 mAbs	1062:1075	anti-MUC1 mAbs	1062:1075	The results indicated that anti-MUC1 mAbs have diverse specificities but can be classified into a few characteristic groups based on their binding pattern toward glycopeptides in some cases having a specific glycan at unique glycosylation sites.
31719620	1	21	theme	MUC1	353:356	arg1	glycosylation					336:348	the differential glycosylation	319:348	the differential glycosylation of MUC1	319:356	Anti-mucin1 (MUC1) antibodies have long been used clinically in cancer diagnosis and therapy and specific bindings of some of them are known to be dependent on the differential glycosylation of MUC1.
31719620	3	22	theme	glycopeptide	793:804	arg1	synthesis					806:814	chemical glycopeptide synthesis	784:814	chemical glycopeptide synthesis	784:814	Here, a total of 20 glycopeptides including the tandem repeat unit of MUC1, APPAHGVTSAPDTRPAPGSTAPPAHGV with GalNAc (Tn-antigen), Galβ1-3GalNAc (T-antigen), NeuAcα2-3Galβ1-3GalNAc (sialyl-T-antigen), or NeuAcα2-6GalNAc (sialyl-Tn-antigen) at each threonine or serine residue were prepared by a combination of chemical glycopeptide synthesis and enzymatic extension of carbohydrate chains.
31719620	6	23	theme	biological	1537:1546	arg1	understanding					1548:1560	the biological understanding	1533:1560	the biological understanding of MUC1	1533:1568	Because the clinical significance of some of these antibodies was already established, the structural features identified by these antibodies as revealed in the present study should provide useful information relevant to their further clinical use and the biological understanding of MUC1.
31719620	4	24	gly	glycopeptides	870:882	arg2	glycopeptides					870:882	These glycopeptides	864:882	These glycopeptides	864:882	These glycopeptides were tested by the enzyme-linked immunosorbent assay (ELISA) for their capacity to bind 13 monoclonal antibodies (mAbs) known to be specific for MUC1.
31719620	2	25	theme	antibodies	434:443	arg1	specificities					407:419	the binding specificities	395:419	the binding specificities of anti-MUC1 antibodies	395:443	However, a systematic comparison of the binding specificities of anti-MUC1 antibodies was not previously conducted.
31719620	4	26	theme	monoclonal	975:984	arg1	mAbs					998:1001	mAbs	998:1001	mAbs	998:1001	These glycopeptides were tested by the enzyme-linked immunosorbent assay (ELISA) for their capacity to bind 13 monoclonal antibodies (mAbs) known to be specific for MUC1.
31719620	4	26	theme	monoclonal	975:984	arg1	antibodies					986:995	13 monoclonal antibodies	972:995	13 monoclonal antibodies (mAbs) known to be specific for MUC1	972:1032	These glycopeptides were tested by the enzyme-linked immunosorbent assay (ELISA) for their capacity to bind 13 monoclonal antibodies (mAbs) known to be specific for MUC1.
31719620	3	27	theme	MUC1	545:548	arg1	T-antigen					620:628	T-antigen	620:628	T-antigen	620:628	Here, a total of 20 glycopeptides including the tandem repeat unit of MUC1, APPAHGVTSAPDTRPAPGSTAPPAHGV with GalNAc (Tn-antigen), Galβ1-3GalNAc (T-antigen), NeuAcα2-3Galβ1-3GalNAc (sialyl-T-antigen), or NeuAcα2-6GalNAc (sialyl-Tn-antigen) at each threonine or serine residue were prepared by a combination of chemical glycopeptide synthesis and enzymatic extension of carbohydrate chains.
31719620	3	27	theme	MUC1	545:548	arg1	unit					537:540	the tandem repeat unit	519:540	the tandem repeat unit of MUC1	519:548	Here, a total of 20 glycopeptides including the tandem repeat unit of MUC1, APPAHGVTSAPDTRPAPGSTAPPAHGV with GalNAc (Tn-antigen), Galβ1-3GalNAc (T-antigen), NeuAcα2-3Galβ1-3GalNAc (sialyl-T-antigen), or NeuAcα2-6GalNAc (sialyl-Tn-antigen) at each threonine or serine residue were prepared by a combination of chemical glycopeptide synthesis and enzymatic extension of carbohydrate chains.
31719620	3	27	theme	MUC1	545:548	arg1	NeuAcα2-6GalNAc					678:692	NeuAcα2-6GalNAc	678:692	NeuAcα2-6GalNAc (sialyl-Tn-antigen)	678:712	Here, a total of 20 glycopeptides including the tandem repeat unit of MUC1, APPAHGVTSAPDTRPAPGSTAPPAHGV with GalNAc (Tn-antigen), Galβ1-3GalNAc (T-antigen), NeuAcα2-3Galβ1-3GalNAc (sialyl-T-antigen), or NeuAcα2-6GalNAc (sialyl-Tn-antigen) at each threonine or serine residue were prepared by a combination of chemical glycopeptide synthesis and enzymatic extension of carbohydrate chains.
31719620	3	27	theme	MUC1	545:548	arg1	NeuAcα2-3Galβ1-3GalNAc					632:653	NeuAcα2-3Galβ1-3GalNAc	632:653	NeuAcα2-3Galβ1-3GalNAc (sialyl-T-antigen)	632:672	Here, a total of 20 glycopeptides including the tandem repeat unit of MUC1, APPAHGVTSAPDTRPAPGSTAPPAHGV with GalNAc (Tn-antigen), Galβ1-3GalNAc (T-antigen), NeuAcα2-3Galβ1-3GalNAc (sialyl-T-antigen), or NeuAcα2-6GalNAc (sialyl-Tn-antigen) at each threonine or serine residue were prepared by a combination of chemical glycopeptide synthesis and enzymatic extension of carbohydrate chains.
31719620	3	27	theme	MUC1	545:548	arg1	Galβ1-3GalNAc					605:617	Galβ1-3GalNAc	605:617	Galβ1-3GalNAc (T-antigen)	605:629	Here, a total of 20 glycopeptides including the tandem repeat unit of MUC1, APPAHGVTSAPDTRPAPGSTAPPAHGV with GalNAc (Tn-antigen), Galβ1-3GalNAc (T-antigen), NeuAcα2-3Galβ1-3GalNAc (sialyl-T-antigen), or NeuAcα2-6GalNAc (sialyl-Tn-antigen) at each threonine or serine residue were prepared by a combination of chemical glycopeptide synthesis and enzymatic extension of carbohydrate chains.
31719620	3	27	theme	MUC1	545:548	arg1	APPAHGVTSAPDTRPAPGSTAPPAHGV					551:577	APPAHGVTSAPDTRPAPGSTAPPAHGV	551:577	APPAHGVTSAPDTRPAPGSTAPPAHGV with GalNAc (Tn-antigen)	551:602	Here, a total of 20 glycopeptides including the tandem repeat unit of MUC1, APPAHGVTSAPDTRPAPGSTAPPAHGV with GalNAc (Tn-antigen), Galβ1-3GalNAc (T-antigen), NeuAcα2-3Galβ1-3GalNAc (sialyl-T-antigen), or NeuAcα2-6GalNAc (sialyl-Tn-antigen) at each threonine or serine residue were prepared by a combination of chemical glycopeptide synthesis and enzymatic extension of carbohydrate chains.
31719620	3	27	theme	MUC1	545:548	arg1	sialyl-T-antigen					656:671	sialyl-T-antigen	656:671	sialyl-T-antigen	656:671	Here, a total of 20 glycopeptides including the tandem repeat unit of MUC1, APPAHGVTSAPDTRPAPGSTAPPAHGV with GalNAc (Tn-antigen), Galβ1-3GalNAc (T-antigen), NeuAcα2-3Galβ1-3GalNAc (sialyl-T-antigen), or NeuAcα2-6GalNAc (sialyl-Tn-antigen) at each threonine or serine residue were prepared by a combination of chemical glycopeptide synthesis and enzymatic extension of carbohydrate chains.
31719620	3	27	theme	MUC1	545:548	arg1	sialyl-Tn-antigen					695:711	sialyl-Tn-antigen	695:711	sialyl-Tn-antigen	695:711	Here, a total of 20 glycopeptides including the tandem repeat unit of MUC1, APPAHGVTSAPDTRPAPGSTAPPAHGV with GalNAc (Tn-antigen), Galβ1-3GalNAc (T-antigen), NeuAcα2-3Galβ1-3GalNAc (sialyl-T-antigen), or NeuAcα2-6GalNAc (sialyl-Tn-antigen) at each threonine or serine residue were prepared by a combination of chemical glycopeptide synthesis and enzymatic extension of carbohydrate chains.
31719620	3	28	with	unit	537:540	arg1	GalNAc					584:589	GalNAc	584:589	GalNAc (Tn-antigen)	584:602	Here, a total of 20 glycopeptides including the tandem repeat unit of MUC1, APPAHGVTSAPDTRPAPGSTAPPAHGV with GalNAc (Tn-antigen), Galβ1-3GalNAc (T-antigen), NeuAcα2-3Galβ1-3GalNAc (sialyl-T-antigen), or NeuAcα2-6GalNAc (sialyl-Tn-antigen) at each threonine or serine residue were prepared by a combination of chemical glycopeptide synthesis and enzymatic extension of carbohydrate chains.
31719620	3	28	with	unit	537:540	arg1	Tn-antigen					592:601	Tn-antigen	592:601	Tn-antigen	592:601	Here, a total of 20 glycopeptides including the tandem repeat unit of MUC1, APPAHGVTSAPDTRPAPGSTAPPAHGV with GalNAc (Tn-antigen), Galβ1-3GalNAc (T-antigen), NeuAcα2-3Galβ1-3GalNAc (sialyl-T-antigen), or NeuAcα2-6GalNAc (sialyl-Tn-antigen) at each threonine or serine residue were prepared by a combination of chemical glycopeptide synthesis and enzymatic extension of carbohydrate chains.
31719620	2	29	theme	anti-MUC1	424:432	arg1	antibodies					434:443	anti-MUC1 antibodies	424:443	anti-MUC1 antibodies	424:443	However, a systematic comparison of the binding specificities of anti-MUC1 antibodies was not previously conducted.
31719620	3	30	theme	serine	735:740	arg1	residue					742:748	each threonine or serine residue	717:748	residue	742:748	Here, a total of 20 glycopeptides including the tandem repeat unit of MUC1, APPAHGVTSAPDTRPAPGSTAPPAHGV with GalNAc (Tn-antigen), Galβ1-3GalNAc (T-antigen), NeuAcα2-3Galβ1-3GalNAc (sialyl-T-antigen), or NeuAcα2-6GalNAc (sialyl-Tn-antigen) at each threonine or serine residue were prepared by a combination of chemical glycopeptide synthesis and enzymatic extension of carbohydrate chains.
31719620	3	31	from	residue	742:748	arg1	unit					537:540	the tandem repeat unit	519:540	the tandem repeat unit of MUC1	519:548	Here, a total of 20 glycopeptides including the tandem repeat unit of MUC1, APPAHGVTSAPDTRPAPGSTAPPAHGV with GalNAc (Tn-antigen), Galβ1-3GalNAc (T-antigen), NeuAcα2-3Galβ1-3GalNAc (sialyl-T-antigen), or NeuAcα2-6GalNAc (sialyl-Tn-antigen) at each threonine or serine residue were prepared by a combination of chemical glycopeptide synthesis and enzymatic extension of carbohydrate chains.
31719620	3	31	from	residue	742:748	arg1	glycopeptides					495:507	20 glycopeptides	492:507	20 glycopeptides including the tandem repeat unit of MUC1, APPAHGVTSAPDTRPAPGSTAPPAHGV with GalNAc (Tn-antigen), Galβ1-3GalNAc (T-antigen), NeuAcα2-3Galβ1-3GalNAc (sialyl-T-antigen), or NeuAcα2-6GalNAc (sialyl-Tn-antigen) at each threonine or serine residue	492:748	Here, a total of 20 glycopeptides including the tandem repeat unit of MUC1, APPAHGVTSAPDTRPAPGSTAPPAHGV with GalNAc (Tn-antigen), Galβ1-3GalNAc (T-antigen), NeuAcα2-3Galβ1-3GalNAc (sialyl-T-antigen), or NeuAcα2-6GalNAc (sialyl-Tn-antigen) at each threonine or serine residue were prepared by a combination of chemical glycopeptide synthesis and enzymatic extension of carbohydrate chains.
31719620	3	31	from	residue	742:748	arg1	NeuAcα2-6GalNAc					678:692	NeuAcα2-6GalNAc	678:692	NeuAcα2-6GalNAc (sialyl-Tn-antigen)	678:712	Here, a total of 20 glycopeptides including the tandem repeat unit of MUC1, APPAHGVTSAPDTRPAPGSTAPPAHGV with GalNAc (Tn-antigen), Galβ1-3GalNAc (T-antigen), NeuAcα2-3Galβ1-3GalNAc (sialyl-T-antigen), or NeuAcα2-6GalNAc (sialyl-Tn-antigen) at each threonine or serine residue were prepared by a combination of chemical glycopeptide synthesis and enzymatic extension of carbohydrate chains.
31719620	3	31	from	residue	742:748	arg1	NeuAcα2-3Galβ1-3GalNAc					632:653	NeuAcα2-3Galβ1-3GalNAc	632:653	NeuAcα2-3Galβ1-3GalNAc (sialyl-T-antigen)	632:672	Here, a total of 20 glycopeptides including the tandem repeat unit of MUC1, APPAHGVTSAPDTRPAPGSTAPPAHGV with GalNAc (Tn-antigen), Galβ1-3GalNAc (T-antigen), NeuAcα2-3Galβ1-3GalNAc (sialyl-T-antigen), or NeuAcα2-6GalNAc (sialyl-Tn-antigen) at each threonine or serine residue were prepared by a combination of chemical glycopeptide synthesis and enzymatic extension of carbohydrate chains.
31719620	3	31	from	residue	742:748	arg1	Galβ1-3GalNAc					605:617	Galβ1-3GalNAc	605:617	Galβ1-3GalNAc (T-antigen)	605:629	Here, a total of 20 glycopeptides including the tandem repeat unit of MUC1, APPAHGVTSAPDTRPAPGSTAPPAHGV with GalNAc (Tn-antigen), Galβ1-3GalNAc (T-antigen), NeuAcα2-3Galβ1-3GalNAc (sialyl-T-antigen), or NeuAcα2-6GalNAc (sialyl-Tn-antigen) at each threonine or serine residue were prepared by a combination of chemical glycopeptide synthesis and enzymatic extension of carbohydrate chains.
31719620	3	31	from	residue	742:748	arg1	APPAHGVTSAPDTRPAPGSTAPPAHGV					551:577	APPAHGVTSAPDTRPAPGSTAPPAHGV	551:577	APPAHGVTSAPDTRPAPGSTAPPAHGV with GalNAc (Tn-antigen)	551:602	Here, a total of 20 glycopeptides including the tandem repeat unit of MUC1, APPAHGVTSAPDTRPAPGSTAPPAHGV with GalNAc (Tn-antigen), Galβ1-3GalNAc (T-antigen), NeuAcα2-3Galβ1-3GalNAc (sialyl-T-antigen), or NeuAcα2-6GalNAc (sialyl-Tn-antigen) at each threonine or serine residue were prepared by a combination of chemical glycopeptide synthesis and enzymatic extension of carbohydrate chains.
31719620	3	31	from	residue	742:748	arg1	total					483:487	a total	481:487	a total of 20 glycopeptides including the tandem repeat unit of MUC1, APPAHGVTSAPDTRPAPGSTAPPAHGV with GalNAc (Tn-antigen), Galβ1-3GalNAc (T-antigen), NeuAcα2-3Galβ1-3GalNAc (sialyl-T-antigen), or NeuAcα2-6GalNAc (sialyl-Tn-antigen) at each threonine or serine residue	481:748	Here, a total of 20 glycopeptides including the tandem repeat unit of MUC1, APPAHGVTSAPDTRPAPGSTAPPAHGV with GalNAc (Tn-antigen), Galβ1-3GalNAc (T-antigen), NeuAcα2-3Galβ1-3GalNAc (sialyl-T-antigen), or NeuAcα2-6GalNAc (sialyl-Tn-antigen) at each threonine or serine residue were prepared by a combination of chemical glycopeptide synthesis and enzymatic extension of carbohydrate chains.
31719620	0	32	theme	Synthesis	27:35	arg1	Products					0:7	Products	0:7	Products of Chemoenzymatic Synthesis Representing MUC1 Tandem Repeat Unit with T-, ST- or STn-antigen	0:100	Products of Chemoenzymatic Synthesis Representing MUC1 Tandem Repeat Unit with T-, ST- or STn-antigen Revealed Distinct Specificities of Anti-MUC1 Antibodies.
31719620	1	33	theme	some	277:280	arg1	bindings					265:272	specific bindings	256:272	specific bindings of some of them	256:288	Anti-mucin1 (MUC1) antibodies have long been used clinically in cancer diagnosis and therapy and specific bindings of some of them are known to be dependent on the differential glycosylation of MUC1.
31719620	5	34	gly	glycopeptides	1197:1209	arg2	glycopeptides					1197:1209	glycopeptides	1197:1209	glycopeptides	1197:1209	The results indicated that anti-MUC1 mAbs have diverse specificities but can be classified into a few characteristic groups based on their binding pattern toward glycopeptides in some cases having a specific glycan at unique glycosylation sites.
31719620	1	35	gly	glycosylation	336:348	arg1	MUC1					353:356	MUC1	353:356	MUC1	353:356	Anti-mucin1 (MUC1) antibodies have long been used clinically in cancer diagnosis and therapy and specific bindings of some of them are known to be dependent on the differential glycosylation of MUC1.
31719620	2	36	theme	binding	399:405	arg1	specificities					407:419	the binding specificities	395:419	the binding specificities of anti-MUC1 antibodies	395:443	However, a systematic comparison of the binding specificities of anti-MUC1 antibodies was not previously conducted.
31719620	2	37	theme	specificities	407:419	arg1	comparison					381:390	a systematic comparison	368:390	a systematic comparison of the binding specificities of anti-MUC1 antibodies	368:443	However, a systematic comparison of the binding specificities of anti-MUC1 antibodies was not previously conducted.
31719620	3	38	theme	threonine	722:730	arg1	residue					742:748	each threonine or serine residue	717:748	residue	742:748	Here, a total of 20 glycopeptides including the tandem repeat unit of MUC1, APPAHGVTSAPDTRPAPGSTAPPAHGV with GalNAc (Tn-antigen), Galβ1-3GalNAc (T-antigen), NeuAcα2-3Galβ1-3GalNAc (sialyl-T-antigen), or NeuAcα2-6GalNAc (sialyl-Tn-antigen) at each threonine or serine residue were prepared by a combination of chemical glycopeptide synthesis and enzymatic extension of carbohydrate chains.
31719620	0	39	theme	Distinct	111:118	arg1	Specificities					120:132	Distinct Specificities	111:132	Distinct Specificities of Anti-MUC1 Antibodies	111:156	Products of Chemoenzymatic Synthesis Representing MUC1 Tandem Repeat Unit with T-, ST- or STn-antigen Revealed Distinct Specificities of Anti-MUC1 Antibodies.
31719620	6	40	theme	clinical	1516:1523	arg1	use					1525:1527	their further clinical use	1502:1527	their further clinical use	1502:1527	Because the clinical significance of some of these antibodies was already established, the structural features identified by these antibodies as revealed in the present study should provide useful information relevant to their further clinical use and the biological understanding of MUC1.
31719620	0	41	theme	MUC1	50:53	arg1	Unit					69:72	MUC1 Tandem Repeat Unit	50:72	MUC1 Tandem Repeat Unit with T-, ST- or STn-antigen	50:100	Products of Chemoenzymatic Synthesis Representing MUC1 Tandem Repeat Unit with T-, ST- or STn-antigen Revealed Distinct Specificities of Anti-MUC1 Antibodies.
31719620	5	42	theme	binding	1174:1180	arg1	pattern					1182:1188	their binding pattern	1168:1188	their binding pattern toward glycopeptides in some cases having a specific glycan at unique glycosylation sites	1168:1278	The results indicated that anti-MUC1 mAbs have diverse specificities but can be classified into a few characteristic groups based on their binding pattern toward glycopeptides in some cases having a specific glycan at unique glycosylation sites.
31719620	0	43	theme	Anti-MUC1	137:145	arg1	Antibodies					147:156	Anti-MUC1 Antibodies	137:156	Anti-MUC1 Antibodies	137:156	Products of Chemoenzymatic Synthesis Representing MUC1 Tandem Repeat Unit with T-, ST- or STn-antigen Revealed Distinct Specificities of Anti-MUC1 Antibodies.
31719620	5	44	gly	glycosylation	1260:1272	arg2	sites					1274:1278	unique glycosylation sites	1253:1278	unique glycosylation sites	1253:1278	The results indicated that anti-MUC1 mAbs have diverse specificities but can be classified into a few characteristic groups based on their binding pattern toward glycopeptides in some cases having a specific glycan at unique glycosylation sites.
31719620	6	45	theme	present	1442:1448	arg1	study					1450:1454	the present study	1438:1454	the present study	1438:1454	Because the clinical significance of some of these antibodies was already established, the structural features identified by these antibodies as revealed in the present study should provide useful information relevant to their further clinical use and the biological understanding of MUC1.
31719620	6	46	theme	further	1508:1514	arg1	use					1525:1527	their further clinical use	1502:1527	their further clinical use	1502:1527	Because the clinical significance of some of these antibodies was already established, the structural features identified by these antibodies as revealed in the present study should provide useful information relevant to their further clinical use and the biological understanding of MUC1.
31719620	5	47	theme	glycosylation	1260:1272	arg1	sites					1274:1278	unique glycosylation sites	1253:1278	unique glycosylation sites	1253:1278	The results indicated that anti-MUC1 mAbs have diverse specificities but can be classified into a few characteristic groups based on their binding pattern toward glycopeptides in some cases having a specific glycan at unique glycosylation sites.
31719620	6	48	theme	some	1318:1321	arg1	significance					1302:1313	the clinical significance	1289:1313	the clinical significance of some of these antibodies	1289:1341	Because the clinical significance of some of these antibodies was already established, the structural features identified by these antibodies as revealed in the present study should provide useful information relevant to their further clinical use and the biological understanding of MUC1.
31719620	5	49	theme	anti-MUC1	1062:1070	arg1	mAbs					1072:1075	anti-MUC1 mAbs	1062:1075	anti-MUC1 mAbs	1062:1075	The results indicated that anti-MUC1 mAbs have diverse specificities but can be classified into a few characteristic groups based on their binding pattern toward glycopeptides in some cases having a specific glycan at unique glycosylation sites.
31719620	1	50	used	used	204:207	arg2	antibodies					178:187	Anti-mucin1 (MUC1) antibodies	159:187	Anti-mucin1 (MUC1) antibodies	159:187	Anti-mucin1 (MUC1) antibodies have long been used clinically in cancer diagnosis and therapy and specific bindings of some of them are known to be dependent on the differential glycosylation of MUC1.
31719620	0	51	theme	Repeat	62:67	arg1	Unit					69:72	MUC1 Tandem Repeat Unit	50:72	MUC1 Tandem Repeat Unit with T-, ST- or STn-antigen	50:100	Products of Chemoenzymatic Synthesis Representing MUC1 Tandem Repeat Unit with T-, ST- or STn-antigen Revealed Distinct Specificities of Anti-MUC1 Antibodies.
31719620	5	52	theme	few	1133:1135	arg1	groups					1152:1157	a few characteristic groups	1131:1157	a few characteristic groups based on their binding pattern toward glycopeptides in some cases having a specific glycan at unique glycosylation sites	1131:1278	The results indicated that anti-MUC1 mAbs have diverse specificities but can be classified into a few characteristic groups based on their binding pattern toward glycopeptides in some cases having a specific glycan at unique glycosylation sites.
31719620	6	53	theme	clinical	1293:1300	arg1	significance					1302:1313	the clinical significance	1289:1313	the clinical significance of some of these antibodies	1289:1341	Because the clinical significance of some of these antibodies was already established, the structural features identified by these antibodies as revealed in the present study should provide useful information relevant to their further clinical use and the biological understanding of MUC1.
31719620	0	54	theme	Tandem	55:60	arg1	Unit					69:72	MUC1 Tandem Repeat Unit	50:72	MUC1 Tandem Repeat Unit with T-, ST- or STn-antigen	50:100	Products of Chemoenzymatic Synthesis Representing MUC1 Tandem Repeat Unit with T-, ST- or STn-antigen Revealed Distinct Specificities of Anti-MUC1 Antibodies.
31719620	5	55	theme	characteristic	1137:1150	arg1	groups					1152:1157	a few characteristic groups	1131:1157	a few characteristic groups based on their binding pattern toward glycopeptides in some cases having a specific glycan at unique glycosylation sites	1131:1278	The results indicated that anti-MUC1 mAbs have diverse specificities but can be classified into a few characteristic groups based on their binding pattern toward glycopeptides in some cases having a specific glycan at unique glycosylation sites.
31719620	0	56	theme	Antibodies	147:156	arg1	Specificities					120:132	Distinct Specificities	111:132	Distinct Specificities of Anti-MUC1 Antibodies	111:156	Products of Chemoenzymatic Synthesis Representing MUC1 Tandem Repeat Unit with T-, ST- or STn-antigen Revealed Distinct Specificities of Anti-MUC1 Antibodies.
31719620	3	57	gly	glycopeptide	793:804	arg2	glycopeptide					793:804	chemical glycopeptide synthesis	784:814	chemical glycopeptide synthesis	784:814	Here, a total of 20 glycopeptides including the tandem repeat unit of MUC1, APPAHGVTSAPDTRPAPGSTAPPAHGV with GalNAc (Tn-antigen), Galβ1-3GalNAc (T-antigen), NeuAcα2-3Galβ1-3GalNAc (sialyl-T-antigen), or NeuAcα2-6GalNAc (sialyl-Tn-antigen) at each threonine or serine residue were prepared by a combination of chemical glycopeptide synthesis and enzymatic extension of carbohydrate chains.
31719620	3	58	theme	chains	856:861	arg1	synthesis					806:814	chemical glycopeptide synthesis	784:814	chemical glycopeptide synthesis	784:814	Here, a total of 20 glycopeptides including the tandem repeat unit of MUC1, APPAHGVTSAPDTRPAPGSTAPPAHGV with GalNAc (Tn-antigen), Galβ1-3GalNAc (T-antigen), NeuAcα2-3Galβ1-3GalNAc (sialyl-T-antigen), or NeuAcα2-6GalNAc (sialyl-Tn-antigen) at each threonine or serine residue were prepared by a combination of chemical glycopeptide synthesis and enzymatic extension of carbohydrate chains.
31719620	3	58	theme	chains	856:861	arg1	extension					830:838	enzymatic extension	820:838	enzymatic extension of carbohydrate chains	820:861	Here, a total of 20 glycopeptides including the tandem repeat unit of MUC1, APPAHGVTSAPDTRPAPGSTAPPAHGV with GalNAc (Tn-antigen), Galβ1-3GalNAc (T-antigen), NeuAcα2-3Galβ1-3GalNAc (sialyl-T-antigen), or NeuAcα2-6GalNAc (sialyl-Tn-antigen) at each threonine or serine residue were prepared by a combination of chemical glycopeptide synthesis and enzymatic extension of carbohydrate chains.
31719620	4	59	theme	immunosorbent	917:929	arg1	ELISA					938:942	ELISA	938:942	ELISA	938:942	These glycopeptides were tested by the enzyme-linked immunosorbent assay (ELISA) for their capacity to bind 13 monoclonal antibodies (mAbs) known to be specific for MUC1.
31719620	4	59	theme	immunosorbent	917:929	arg1	assay					931:935	the enzyme-linked immunosorbent assay	899:935	the enzyme-linked immunosorbent assay (ELISA) for their capacity to bind 13 monoclonal antibodies (mAbs) known to be specific for MUC1	899:1032	These glycopeptides were tested by the enzyme-linked immunosorbent assay (ELISA) for their capacity to bind 13 monoclonal antibodies (mAbs) known to be specific for MUC1.
31719620	3	60	theme	chemical	784:791	arg1	synthesis					806:814	chemical glycopeptide synthesis	784:814	chemical glycopeptide synthesis	784:814	Here, a total of 20 glycopeptides including the tandem repeat unit of MUC1, APPAHGVTSAPDTRPAPGSTAPPAHGV with GalNAc (Tn-antigen), Galβ1-3GalNAc (T-antigen), NeuAcα2-3Galβ1-3GalNAc (sialyl-T-antigen), or NeuAcα2-6GalNAc (sialyl-Tn-antigen) at each threonine or serine residue were prepared by a combination of chemical glycopeptide synthesis and enzymatic extension of carbohydrate chains.
31719620	4	61	theme	enzyme-linked	903:915	arg1	ELISA					938:942	ELISA	938:942	ELISA	938:942	These glycopeptides were tested by the enzyme-linked immunosorbent assay (ELISA) for their capacity to bind 13 monoclonal antibodies (mAbs) known to be specific for MUC1.
31719620	4	61	theme	enzyme-linked	903:915	arg1	assay					931:935	the enzyme-linked immunosorbent assay	899:935	the enzyme-linked immunosorbent assay (ELISA) for their capacity to bind 13 monoclonal antibodies (mAbs) known to be specific for MUC1	899:1032	These glycopeptides were tested by the enzyme-linked immunosorbent assay (ELISA) for their capacity to bind 13 monoclonal antibodies (mAbs) known to be specific for MUC1.
31719620	3	62	theme	tandem	523:528	arg1	unit					537:540	the tandem repeat unit	519:540	the tandem repeat unit of MUC1	519:548	Here, a total of 20 glycopeptides including the tandem repeat unit of MUC1, APPAHGVTSAPDTRPAPGSTAPPAHGV with GalNAc (Tn-antigen), Galβ1-3GalNAc (T-antigen), NeuAcα2-3Galβ1-3GalNAc (sialyl-T-antigen), or NeuAcα2-6GalNAc (sialyl-Tn-antigen) at each threonine or serine residue were prepared by a combination of chemical glycopeptide synthesis and enzymatic extension of carbohydrate chains.
31719620	5	63	from	pattern	1182:1188	arg1	cases					1219:1223	some cases	1214:1223	some cases having a specific glycan at unique glycosylation sites	1214:1278	The results indicated that anti-MUC1 mAbs have diverse specificities but can be classified into a few characteristic groups based on their binding pattern toward glycopeptides in some cases having a specific glycan at unique glycosylation sites.
31719620	6	64	theme	relevant	1490:1497	arg1	information					1478:1488	useful information	1471:1488	useful information relevant to their further clinical use	1471:1527	Because the clinical significance of some of these antibodies was already established, the structural features identified by these antibodies as revealed in the present study should provide useful information relevant to their further clinical use and the biological understanding of MUC1.
31719620	1	65	theme	cancer	223:228	arg1	diagnosis					230:238	cancer diagnosis	223:238	cancer diagnosis	223:238	Anti-mucin1 (MUC1) antibodies have long been used clinically in cancer diagnosis and therapy and specific bindings of some of them are known to be dependent on the differential glycosylation of MUC1.
31719620	6	66	theme	useful	1471:1476	arg1	information					1478:1488	useful information	1471:1488	useful information relevant to their further clinical use	1471:1527	Because the clinical significance of some of these antibodies was already established, the structural features identified by these antibodies as revealed in the present study should provide useful information relevant to their further clinical use and the biological understanding of MUC1.
31719620	3	67	theme	carbohydrate	843:854	arg1	chains					856:861	carbohydrate chains	843:861	carbohydrate chains	843:861	Here, a total of 20 glycopeptides including the tandem repeat unit of MUC1, APPAHGVTSAPDTRPAPGSTAPPAHGV with GalNAc (Tn-antigen), Galβ1-3GalNAc (T-antigen), NeuAcα2-3Galβ1-3GalNAc (sialyl-T-antigen), or NeuAcα2-6GalNAc (sialyl-Tn-antigen) at each threonine or serine residue were prepared by a combination of chemical glycopeptide synthesis and enzymatic extension of carbohydrate chains.
31719620	3	68	theme	synthesis	806:814	arg1	combination					769:779	a combination	767:779	a combination of chemical glycopeptide synthesis and enzymatic extension of carbohydrate chains	767:861	Here, a total of 20 glycopeptides including the tandem repeat unit of MUC1, APPAHGVTSAPDTRPAPGSTAPPAHGV with GalNAc (Tn-antigen), Galβ1-3GalNAc (T-antigen), NeuAcα2-3Galβ1-3GalNAc (sialyl-T-antigen), or NeuAcα2-6GalNAc (sialyl-Tn-antigen) at each threonine or serine residue were prepared by a combination of chemical glycopeptide synthesis and enzymatic extension of carbohydrate chains.
31719620	0	69	theme	Chemoenzymatic	12:25	arg1	Synthesis					27:35	Chemoenzymatic Synthesis	12:35	Chemoenzymatic Synthesis Representing MUC1 Tandem Repeat Unit with T-, ST- or STn-antigen	12:100	Products of Chemoenzymatic Synthesis Representing MUC1 Tandem Repeat Unit with T-, ST- or STn-antigen Revealed Distinct Specificities of Anti-MUC1 Antibodies.
31719620	3	70	theme	glycopeptides	495:507	arg1	total					483:487	a total	481:487	a total of 20 glycopeptides including the tandem repeat unit of MUC1, APPAHGVTSAPDTRPAPGSTAPPAHGV with GalNAc (Tn-antigen), Galβ1-3GalNAc (T-antigen), NeuAcα2-3Galβ1-3GalNAc (sialyl-T-antigen), or NeuAcα2-6GalNAc (sialyl-Tn-antigen) at each threonine or serine residue	481:748	Here, a total of 20 glycopeptides including the tandem repeat unit of MUC1, APPAHGVTSAPDTRPAPGSTAPPAHGV with GalNAc (Tn-antigen), Galβ1-3GalNAc (T-antigen), NeuAcα2-3Galβ1-3GalNAc (sialyl-T-antigen), or NeuAcα2-6GalNAc (sialyl-Tn-antigen) at each threonine or serine residue were prepared by a combination of chemical glycopeptide synthesis and enzymatic extension of carbohydrate chains.
31719620	5	71	theme	specific	1234:1241	arg1	glycan					1243:1248	a specific glycan	1232:1248	a specific glycan	1232:1248	The results indicated that anti-MUC1 mAbs have diverse specificities but can be classified into a few characteristic groups based on their binding pattern toward glycopeptides in some cases having a specific glycan at unique glycosylation sites.
31826327	0	0	theme	trilayer	70:77	arg1	scaffold					79:86	an implantable porous trilayer scaffold	48:86	an implantable porous trilayer scaffold	48:86	Design, construction, and biological testing of an implantable porous trilayer scaffold for repairing osteoarthritic cartilage.
31826327	5	1	from	bone	789:792	arg1	area					808:811	the target area	797:811	the target area after scaffold implantation	797:839	One of its layers includes hydroxyapatite, a bioactive component that facilitates the integration of growing tissue on local bone in the target area after scaffold implantation.
31826327	3	2	theme	porous	464:469	arg1	scaffold					496:503	a porous, chitosan/collagen-based scaffold	462:503	a porous, chitosan/collagen-based scaffold as an implant to repair damaged articular cartilage	462:555	Here, the design, assembly, and biological testing of a porous, chitosan/collagen-based scaffold as an implant to repair damaged articular cartilage is reported.
31826327	3	3	dep	porous	464:469	arg1	chitosan/collagen-based					472:494	chitosan/collagen-based	472:494	chitosan/collagen-based	472:494	Here, the design, assembly, and biological testing of a porous, chitosan/collagen-based scaffold as an implant to repair damaged articular cartilage is reported.
31826327	0	4	theme	porous	63:68	arg1	scaffold					79:86	an implantable porous trilayer scaffold	48:86	an implantable porous trilayer scaffold	48:86	Design, construction, and biological testing of an implantable porous trilayer scaffold for repairing osteoarthritic cartilage.
31826327	8	5	theme	cartilage	1459:1467	arg1	engineering					1476:1486	cartilage tissue engineering	1459:1486	cartilage tissue engineering	1459:1486	These encouraging results highlight the potential of this porous chitosan/collagen scaffold for clinical applications in cartilage tissue engineering.
31826327	6	6	theme	C28	1064:1066	arg1	line					1073:1076	a human chondrocyte C28 cell line	1044:1076	a human chondrocyte C28 cell line	1044:1076	The scaffold was evaluated for surface morphology; rheological performance (storage, loss, complex, and time-relaxation moduli at 1 kHz); physiological stability; in vitro activity and cytotoxicity (on a human chondrocyte C28 cell line); and in vivo performance (tissue growth and biodegradability), in a murine model of osteoarthritis.
31826327	1	7	theme	cartilage	167:175	arg1	repair					177:182	cartilage repair	167:182	cartilage repair	167:182	Various tissue engineering systems for cartilage repair have been designed and tested over the past two decades, leading to the development of many promising cartilage grafts.
31826327	6	8	dep	performance	1092:1102	arg1	growth					1112:1117	tissue growth	1105:1117	tissue growth	1105:1117	The scaffold was evaluated for surface morphology; rheological performance (storage, loss, complex, and time-relaxation moduli at 1 kHz); physiological stability; in vitro activity and cytotoxicity (on a human chondrocyte C28 cell line); and in vivo performance (tissue growth and biodegradability), in a murine model of osteoarthritis.
31826327	6	8	dep	performance	1092:1102	arg1	biodegradability					1123:1138	biodegradability	1123:1138	biodegradability	1123:1138	The scaffold was evaluated for surface morphology; rheological performance (storage, loss, complex, and time-relaxation moduli at 1 kHz); physiological stability; in vitro activity and cytotoxicity (on a human chondrocyte C28 cell line); and in vivo performance (tissue growth and biodegradability), in a murine model of osteoarthritis.
31826327	5	9	theme	bioactive	709:717	arg1	hydroxyapatite					691:704	hydroxyapatite	691:704	hydroxyapatite	691:704	One of its layers includes hydroxyapatite, a bioactive component that facilitates the integration of growing tissue on local bone in the target area after scaffold implantation.
31826327	5	9	theme	bioactive	709:717	arg1	component					719:727	a bioactive component	707:727	a bioactive component that facilitates the integration of growing tissue on local bone in the target area after scaffold implantation	707:839	One of its layers includes hydroxyapatite, a bioactive component that facilitates the integration of growing tissue on local bone in the target area after scaffold implantation.
31826327	6	10	theme	rheological	893:903	arg1	performance					905:915	rheological performance	893:915	rheological performance (storage, loss, complex, and time-relaxation moduli at 1 kHz)	893:977	The scaffold was evaluated for surface morphology; rheological performance (storage, loss, complex, and time-relaxation moduli at 1 kHz); physiological stability; in vitro activity and cytotoxicity (on a human chondrocyte C28 cell line); and in vivo performance (tissue growth and biodegradability), in a murine model of osteoarthritis.
31826327	6	11	theme	tissue	1105:1110	arg1	growth					1112:1117	tissue growth	1105:1117	tissue growth	1105:1117	The scaffold was evaluated for surface morphology; rheological performance (storage, loss, complex, and time-relaxation moduli at 1 kHz); physiological stability; in vitro activity and cytotoxicity (on a human chondrocyte C28 cell line); and in vivo performance (tissue growth and biodegradability), in a murine model of osteoarthritis.
31826327	6	12	theme	in	1084:1085	arg1	performance					1092:1102	in vivo performance	1084:1102	in vivo performance (tissue growth and biodegradability)	1084:1139	The scaffold was evaluated for surface morphology; rheological performance (storage, loss, complex, and time-relaxation moduli at 1 kHz); physiological stability; in vitro activity and cytotoxicity (on a human chondrocyte C28 cell line); and in vivo performance (tissue growth and biodegradability), in a murine model of osteoarthritis.
31826327	6	13	dep	storage	918:924	arg1	complex					933:939	complex	933:939	complex	933:939	The scaffold was evaluated for surface morphology; rheological performance (storage, loss, complex, and time-relaxation moduli at 1 kHz); physiological stability; in vitro activity and cytotoxicity (on a human chondrocyte C28 cell line); and in vivo performance (tissue growth and biodegradability), in a murine model of osteoarthritis.
31826327	6	13	dep	storage	918:924	arg1	loss					927:930	loss	927:930	loss	927:930	The scaffold was evaluated for surface morphology; rheological performance (storage, loss, complex, and time-relaxation moduli at 1 kHz); physiological stability; in vitro activity and cytotoxicity (on a human chondrocyte C28 cell line); and in vivo performance (tissue growth and biodegradability), in a murine model of osteoarthritis.
31826327	6	13	dep	storage	918:924	arg1	moduli					962:967	time-relaxation moduli	946:967	time-relaxation moduli	946:967	The scaffold was evaluated for surface morphology; rheological performance (storage, loss, complex, and time-relaxation moduli at 1 kHz); physiological stability; in vitro activity and cytotoxicity (on a human chondrocyte C28 cell line); and in vivo performance (tissue growth and biodegradability), in a murine model of osteoarthritis.
31826327	0	14	theme	scaffold	79:86	arg1	construction					8:19	construction	8:19	construction	8:19	Design, construction, and biological testing of an implantable porous trilayer scaffold for repairing osteoarthritic cartilage.
31826327	0	14	theme	scaffold	79:86	arg1	Design					0:5	Design	0:5	Design	0:5	Design, construction, and biological testing of an implantable porous trilayer scaffold for repairing osteoarthritic cartilage.
31826327	0	14	theme	scaffold	79:86	arg1	testing					37:43	biological testing	26:43	biological testing of an implantable porous trilayer scaffold for repairing osteoarthritic cartilage	26:125	Design, construction, and biological testing of an implantable porous trilayer scaffold for repairing osteoarthritic cartilage.
31826327	6	15	from	stability	994:1002	arg1	line					1073:1076	a human chondrocyte C28 cell line	1044:1076	a human chondrocyte C28 cell line	1044:1076	The scaffold was evaluated for surface morphology; rheological performance (storage, loss, complex, and time-relaxation moduli at 1 kHz); physiological stability; in vitro activity and cytotoxicity (on a human chondrocyte C28 cell line); and in vivo performance (tissue growth and biodegradability), in a murine model of osteoarthritis.
31826327	6	16	theme	chondrocyte	1052:1062	arg1	line					1073:1076	a human chondrocyte C28 cell line	1044:1076	a human chondrocyte C28 cell line	1044:1076	The scaffold was evaluated for surface morphology; rheological performance (storage, loss, complex, and time-relaxation moduli at 1 kHz); physiological stability; in vitro activity and cytotoxicity (on a human chondrocyte C28 cell line); and in vivo performance (tissue growth and biodegradability), in a murine model of osteoarthritis.
31826327	6	17	dep	performance	905:915	arg1	storage					918:924	storage	918:924	storage	918:924	The scaffold was evaluated for surface morphology; rheological performance (storage, loss, complex, and time-relaxation moduli at 1 kHz); physiological stability; in vitro activity and cytotoxicity (on a human chondrocyte C28 cell line); and in vivo performance (tissue growth and biodegradability), in a murine model of osteoarthritis.
31826327	0	18	theme	osteoarthritic	102:115	arg1	cartilage					117:125	osteoarthritic cartilage	102:125	osteoarthritic cartilage	102:125	Design, construction, and biological testing of an implantable porous trilayer scaffold for repairing osteoarthritic cartilage.
31826327	8	19	theme	tissue	1469:1474	arg1	engineering					1476:1486	cartilage tissue engineering	1459:1486	cartilage tissue engineering	1459:1486	These encouraging results highlight the potential of this porous chitosan/collagen scaffold for clinical applications in cartilage tissue engineering.
31826327	7	20	theme	days	1307:1310	arg1	postimplantation					1312:1327	35 days postimplantation	1304:1327	35 days postimplantation	1304:1327	The scaffold was shown to be mechanically resistant and noncytotoxic, favored tissue growth in vivo, and remained stable for 35 days postimplantation in mice.
31826327	4	21	theme	cartilage	646:654	arg1	tissue					656:661	natural cartilage tissue	638:661	natural cartilage tissue	638:661	Its gradient composition and trilayer structure mimic variations in natural cartilage tissue.
31826327	6	22	from	morphology	881:890	arg1	line					1073:1076	a human chondrocyte C28 cell line	1044:1076	a human chondrocyte C28 cell line	1044:1076	The scaffold was evaluated for surface morphology; rheological performance (storage, loss, complex, and time-relaxation moduli at 1 kHz); physiological stability; in vitro activity and cytotoxicity (on a human chondrocyte C28 cell line); and in vivo performance (tissue growth and biodegradability), in a murine model of osteoarthritis.
31826327	6	23	theme	surface	873:879	arg1	morphology					881:890	surface morphology	873:890	surface morphology	873:890	The scaffold was evaluated for surface morphology; rheological performance (storage, loss, complex, and time-relaxation moduli at 1 kHz); physiological stability; in vitro activity and cytotoxicity (on a human chondrocyte C28 cell line); and in vivo performance (tissue growth and biodegradability), in a murine model of osteoarthritis.
31826327	5	24	theme	tissue	773:778	arg1	integration					750:760	the integration	746:760	the integration of growing tissue on local bone in the target area after scaffold implantation	746:839	One of its layers includes hydroxyapatite, a bioactive component that facilitates the integration of growing tissue on local bone in the target area after scaffold implantation.
31826327	4	25	theme	gradient	574:581	arg1	composition					583:593	gradient composition	574:593	gradient composition	574:593	Its gradient composition and trilayer structure mimic variations in natural cartilage tissue.
31826327	6	26	theme	human	1046:1050	arg1	line					1073:1076	a human chondrocyte C28 cell line	1044:1076	a human chondrocyte C28 cell line	1044:1076	The scaffold was evaluated for surface morphology; rheological performance (storage, loss, complex, and time-relaxation moduli at 1 kHz); physiological stability; in vitro activity and cytotoxicity (on a human chondrocyte C28 cell line); and in vivo performance (tissue growth and biodegradability), in a murine model of osteoarthritis.
31826327	4	27	theme	natural	638:644	arg1	tissue					656:661	natural cartilage tissue	638:661	natural cartilage tissue	638:661	Its gradient composition and trilayer structure mimic variations in natural cartilage tissue.
31826327	5	28	theme	growing	765:771	arg1	tissue					773:778	growing tissue	765:778	growing tissue	765:778	One of its layers includes hydroxyapatite, a bioactive component that facilitates the integration of growing tissue on local bone in the target area after scaffold implantation.
31826327	6	29	from	performance	905:915	arg1	line					1073:1076	a human chondrocyte C28 cell line	1044:1076	a human chondrocyte C28 cell line	1044:1076	The scaffold was evaluated for surface morphology; rheological performance (storage, loss, complex, and time-relaxation moduli at 1 kHz); physiological stability; in vitro activity and cytotoxicity (on a human chondrocyte C28 cell line); and in vivo performance (tissue growth and biodegradability), in a murine model of osteoarthritis.
31826327	3	30	theme	articular	537:545	arg1	cartilage					547:555	repair damaged articular cartilage	522:555	repair damaged articular cartilage	522:555	Here, the design, assembly, and biological testing of a porous, chitosan/collagen-based scaffold as an implant to repair damaged articular cartilage is reported.
31826327	6	31	from	kHz	974:976	arg1	complex					933:939	complex	933:939	complex	933:939	The scaffold was evaluated for surface morphology; rheological performance (storage, loss, complex, and time-relaxation moduli at 1 kHz); physiological stability; in vitro activity and cytotoxicity (on a human chondrocyte C28 cell line); and in vivo performance (tissue growth and biodegradability), in a murine model of osteoarthritis.
31826327	6	31	from	kHz	974:976	arg1	loss					927:930	loss	927:930	loss	927:930	The scaffold was evaluated for surface morphology; rheological performance (storage, loss, complex, and time-relaxation moduli at 1 kHz); physiological stability; in vitro activity and cytotoxicity (on a human chondrocyte C28 cell line); and in vivo performance (tissue growth and biodegradability), in a murine model of osteoarthritis.
31826327	6	31	from	kHz	974:976	arg1	moduli					962:967	time-relaxation moduli	946:967	time-relaxation moduli	946:967	The scaffold was evaluated for surface morphology; rheological performance (storage, loss, complex, and time-relaxation moduli at 1 kHz); physiological stability; in vitro activity and cytotoxicity (on a human chondrocyte C28 cell line); and in vivo performance (tissue growth and biodegradability), in a murine model of osteoarthritis.
31826327	2	32	theme	optimal	353:359	arg1	system					361:366	an optimal system	350:366	an optimal system to restore damaged articular cartilage	350:405	However, no one has yet succeeded in devising an optimal system to restore damaged articular cartilage.
31826327	5	33	theme	target	801:806	arg1	area					808:811	the target area	797:811	the target area after scaffold implantation	797:839	One of its layers includes hydroxyapatite, a bioactive component that facilitates the integration of growing tissue on local bone in the target area after scaffold implantation.
31826327	1	34	theme	many	271:274	arg1	grafts					296:301	many promising cartilage grafts	271:301	many promising cartilage grafts	271:301	Various tissue engineering systems for cartilage repair have been designed and tested over the past two decades, leading to the development of many promising cartilage grafts.
31826327	6	35	from	activity	1014:1021	arg1	line					1073:1076	a human chondrocyte C28 cell line	1044:1076	a human chondrocyte C28 cell line	1044:1076	The scaffold was evaluated for surface morphology; rheological performance (storage, loss, complex, and time-relaxation moduli at 1 kHz); physiological stability; in vitro activity and cytotoxicity (on a human chondrocyte C28 cell line); and in vivo performance (tissue growth and biodegradability), in a murine model of osteoarthritis.
31826327	3	36	theme	repair	522:527	arg1	cartilage					547:555	repair damaged articular cartilage	522:555	repair damaged articular cartilage	522:555	Here, the design, assembly, and biological testing of a porous, chitosan/collagen-based scaffold as an implant to repair damaged articular cartilage is reported.
31826327	4	37	from	variations	624:633	arg1	tissue					656:661	natural cartilage tissue	638:661	natural cartilage tissue	638:661	Its gradient composition and trilayer structure mimic variations in natural cartilage tissue.
31826327	1	38	theme	promising	276:284	arg1	grafts					296:301	many promising cartilage grafts	271:301	many promising cartilage grafts	271:301	Various tissue engineering systems for cartilage repair have been designed and tested over the past two decades, leading to the development of many promising cartilage grafts.
31826327	3	39	theme	damaged	529:535	arg1	cartilage					547:555	repair damaged articular cartilage	522:555	repair damaged articular cartilage	522:555	Here, the design, assembly, and biological testing of a porous, chitosan/collagen-based scaffold as an implant to repair damaged articular cartilage is reported.
31826327	5	40	theme	scaffold	819:826	arg1	implantation					828:839	scaffold implantation	819:839	scaffold implantation	819:839	One of its layers includes hydroxyapatite, a bioactive component that facilitates the integration of growing tissue on local bone in the target area after scaffold implantation.
31826327	0	41	theme	biological	26:35	arg1	testing					37:43	biological testing	26:43	biological testing of an implantable porous trilayer scaffold for repairing osteoarthritic cartilage	26:125	Design, construction, and biological testing of an implantable porous trilayer scaffold for repairing osteoarthritic cartilage.
31826327	1	42	theme	cartilage	286:294	arg1	grafts					296:301	many promising cartilage grafts	271:301	many promising cartilage grafts	271:301	Various tissue engineering systems for cartilage repair have been designed and tested over the past two decades, leading to the development of many promising cartilage grafts.
31826327	8	43	theme	scaffold	1421:1428	arg1	potential					1378:1386	the potential	1374:1386	the potential of this porous chitosan/collagen scaffold for clinical applications in cartilage tissue engineering	1374:1486	These encouraging results highlight the potential of this porous chitosan/collagen scaffold for clinical applications in cartilage tissue engineering.
31826327	6	44	theme	osteoarthritis	1163:1176	arg1	model					1154:1158	a murine model	1145:1158	a murine model of osteoarthritis	1145:1176	The scaffold was evaluated for surface morphology; rheological performance (storage, loss, complex, and time-relaxation moduli at 1 kHz); physiological stability; in vitro activity and cytotoxicity (on a human chondrocyte C28 cell line); and in vivo performance (tissue growth and biodegradability), in a murine model of osteoarthritis.
31826327	6	45	from	performance	1092:1102	arg1	line					1073:1076	a human chondrocyte C28 cell line	1044:1076	a human chondrocyte C28 cell line	1044:1076	The scaffold was evaluated for surface morphology; rheological performance (storage, loss, complex, and time-relaxation moduli at 1 kHz); physiological stability; in vitro activity and cytotoxicity (on a human chondrocyte C28 cell line); and in vivo performance (tissue growth and biodegradability), in a murine model of osteoarthritis.
31826327	2	46	theme	articular	387:395	arg1	cartilage					397:405	damaged articular cartilage	379:405	damaged articular cartilage	379:405	However, no one has yet succeeded in devising an optimal system to restore damaged articular cartilage.
31826327	1	47	theme	grafts	296:301	arg1	development					256:266	the development	252:266	the development of many promising cartilage grafts	252:301	Various tissue engineering systems for cartilage repair have been designed and tested over the past two decades, leading to the development of many promising cartilage grafts.
31826327	6	48	theme	physiological	980:992	arg1	stability					994:1002	physiological stability	980:1002	physiological stability	980:1002	The scaffold was evaluated for surface morphology; rheological performance (storage, loss, complex, and time-relaxation moduli at 1 kHz); physiological stability; in vitro activity and cytotoxicity (on a human chondrocyte C28 cell line); and in vivo performance (tissue growth and biodegradability), in a murine model of osteoarthritis.
31826327	2	49	theme	damaged	379:385	arg1	cartilage					397:405	damaged articular cartilage	379:405	damaged articular cartilage	379:405	However, no one has yet succeeded in devising an optimal system to restore damaged articular cartilage.
31826327	5	50	theme	local	783:787	arg1	bone					789:792	local bone	783:792	local bone in the target area after scaffold implantation	783:839	One of its layers includes hydroxyapatite, a bioactive component that facilitates the integration of growing tissue on local bone in the target area after scaffold implantation.
31826327	6	51	theme	in	1005:1006	arg1	activity					1014:1021	in vitro activity	1005:1021	in vitro activity	1005:1021	The scaffold was evaluated for surface morphology; rheological performance (storage, loss, complex, and time-relaxation moduli at 1 kHz); physiological stability; in vitro activity and cytotoxicity (on a human chondrocyte C28 cell line); and in vivo performance (tissue growth and biodegradability), in a murine model of osteoarthritis.
31826327	6	52	theme	time-relaxation	946:960	arg1	moduli					962:967	time-relaxation moduli	946:967	time-relaxation moduli	946:967	The scaffold was evaluated for surface morphology; rheological performance (storage, loss, complex, and time-relaxation moduli at 1 kHz); physiological stability; in vitro activity and cytotoxicity (on a human chondrocyte C28 cell line); and in vivo performance (tissue growth and biodegradability), in a murine model of osteoarthritis.
31826327	8	53	theme	clinical	1434:1441	arg1	applications					1443:1454	clinical applications	1434:1454	clinical applications in cartilage tissue engineering	1434:1486	These encouraging results highlight the potential of this porous chitosan/collagen scaffold for clinical applications in cartilage tissue engineering.
31826327	6	54	theme	cell	1068:1071	arg1	line					1073:1076	a human chondrocyte C28 cell line	1044:1076	a human chondrocyte C28 cell line	1044:1076	The scaffold was evaluated for surface morphology; rheological performance (storage, loss, complex, and time-relaxation moduli at 1 kHz); physiological stability; in vitro activity and cytotoxicity (on a human chondrocyte C28 cell line); and in vivo performance (tissue growth and biodegradability), in a murine model of osteoarthritis.
31826327	7	55	theme	tissue	1257:1262	arg1	growth					1264:1269	tissue growth	1257:1269	tissue growth	1257:1269	The scaffold was shown to be mechanically resistant and noncytotoxic, favored tissue growth in vivo, and remained stable for 35 days postimplantation in mice.
31826327	8	56	theme	chitosan/collagen	1403:1419	arg1	scaffold					1421:1428	this porous chitosan/collagen scaffold	1391:1428	this porous chitosan/collagen scaffold	1391:1428	These encouraging results highlight the potential of this porous chitosan/collagen scaffold for clinical applications in cartilage tissue engineering.
31826327	6	57	theme	murine	1147:1152	arg1	model					1154:1158	a murine model	1145:1158	a murine model of osteoarthritis	1145:1176	The scaffold was evaluated for surface morphology; rheological performance (storage, loss, complex, and time-relaxation moduli at 1 kHz); physiological stability; in vitro activity and cytotoxicity (on a human chondrocyte C28 cell line); and in vivo performance (tissue growth and biodegradability), in a murine model of osteoarthritis.
31826327	8	58	theme	porous	1396:1401	arg1	scaffold					1421:1428	this porous chitosan/collagen scaffold	1391:1428	this porous chitosan/collagen scaffold	1391:1428	These encouraging results highlight the potential of this porous chitosan/collagen scaffold for clinical applications in cartilage tissue engineering.
31826327	8	59	theme	encouraging	1344:1354	arg1	results					1356:1362	These encouraging results	1338:1362	These encouraging results	1338:1362	These encouraging results highlight the potential of this porous chitosan/collagen scaffold for clinical applications in cartilage tissue engineering.
31826327	6	60	from	cytotoxicity	1027:1038	arg1	line					1073:1076	a human chondrocyte C28 cell line	1044:1076	a human chondrocyte C28 cell line	1044:1076	The scaffold was evaluated for surface morphology; rheological performance (storage, loss, complex, and time-relaxation moduli at 1 kHz); physiological stability; in vitro activity and cytotoxicity (on a human chondrocyte C28 cell line); and in vivo performance (tissue growth and biodegradability), in a murine model of osteoarthritis.
31826327	1	61	theme	Various	128:134	arg1	systems					155:161	Various tissue engineering systems	128:161	Various tissue engineering systems for cartilage repair	128:182	Various tissue engineering systems for cartilage repair have been designed and tested over the past two decades, leading to the development of many promising cartilage grafts.
31826327	1	62	theme	past	223:226	arg1	decades					232:238	the past two decades	219:238	the past two decades	219:238	Various tissue engineering systems for cartilage repair have been designed and tested over the past two decades, leading to the development of many promising cartilage grafts.
31826327	5	63	theme	layers	675:680	arg1	One					664:666	One	664:666	One	664:666	One of its layers includes hydroxyapatite, a bioactive component that facilitates the integration of growing tissue on local bone in the target area after scaffold implantation.
31826327	5	63	theme	layers	675:680	arg1	layers					675:680	its layers	671:680	its layers	671:680	One of its layers includes hydroxyapatite, a bioactive component that facilitates the integration of growing tissue on local bone in the target area after scaffold implantation.
31826327	8	64	from	applications	1443:1454	arg1	engineering					1476:1486	cartilage tissue engineering	1459:1486	cartilage tissue engineering	1459:1486	These encouraging results highlight the potential of this porous chitosan/collagen scaffold for clinical applications in cartilage tissue engineering.
31826327	1	65	theme	tissue	136:141	arg1	systems					155:161	Various tissue engineering systems	128:161	Various tissue engineering systems for cartilage repair	128:182	Various tissue engineering systems for cartilage repair have been designed and tested over the past two decades, leading to the development of many promising cartilage grafts.
31826327	3	66	theme	biological	440:449	arg1	testing					451:457	biological testing	440:457	biological testing	440:457	Here, the design, assembly, and biological testing of a porous, chitosan/collagen-based scaffold as an implant to repair damaged articular cartilage is reported.
31826327	0	67	theme	implantable	51:61	arg1	scaffold					79:86	an implantable porous trilayer scaffold	48:86	an implantable porous trilayer scaffold	48:86	Design, construction, and biological testing of an implantable porous trilayer scaffold for repairing osteoarthritic cartilage.
31826327	5	68	from	integration	750:760	arg1	bone					789:792	local bone	783:792	local bone in the target area after scaffold implantation	783:839	One of its layers includes hydroxyapatite, a bioactive component that facilitates the integration of growing tissue on local bone in the target area after scaffold implantation.
31826327	1	69	theme	engineering	143:153	arg1	systems					155:161	Various tissue engineering systems	128:161	Various tissue engineering systems for cartilage repair	128:182	Various tissue engineering systems for cartilage repair have been designed and tested over the past two decades, leading to the development of many promising cartilage grafts.
31826327	6	70	dep	in	1084:1085	arg1	vivo					1087:1090	vivo	1087:1090	vivo	1087:1090	The scaffold was evaluated for surface morphology; rheological performance (storage, loss, complex, and time-relaxation moduli at 1 kHz); physiological stability; in vitro activity and cytotoxicity (on a human chondrocyte C28 cell line); and in vivo performance (tissue growth and biodegradability), in a murine model of osteoarthritis.
31826327	4	71	theme	trilayer	599:606	arg1	structure					608:616	trilayer structure	599:616	trilayer structure	599:616	Its gradient composition and trilayer structure mimic variations in natural cartilage tissue.
31826327	3	72	theme	scaffold	496:503	arg1	assembly					426:433	assembly	426:433	assembly	426:433	Here, the design, assembly, and biological testing of a porous, chitosan/collagen-based scaffold as an implant to repair damaged articular cartilage is reported.
31826327	3	72	theme	scaffold	496:503	arg1	design					418:423	design	418:423	design	418:423	Here, the design, assembly, and biological testing of a porous, chitosan/collagen-based scaffold as an implant to repair damaged articular cartilage is reported.
31826327	3	72	theme	scaffold	496:503	arg1	testing					451:457	biological testing	440:457	biological testing	440:457	Here, the design, assembly, and biological testing of a porous, chitosan/collagen-based scaffold as an implant to repair damaged articular cartilage is reported.
31826327	6	73	dep	in	1005:1006	arg1	vitro					1008:1012	vitro	1008:1012	vitro	1008:1012	The scaffold was evaluated for surface morphology; rheological performance (storage, loss, complex, and time-relaxation moduli at 1 kHz); physiological stability; in vitro activity and cytotoxicity (on a human chondrocyte C28 cell line); and in vivo performance (tissue growth and biodegradability), in a murine model of osteoarthritis.
31052177	5	0	theme	GI	763:764	arg1	morphology					766:775	a significantly different GI morphology	737:775	a significantly different GI morphology in Shank3 knock-out (KO) mice	737:805	Here, we show expression in gastrointestinal (GI) epithelium and report a significantly different GI morphology in Shank3 knock-out (KO) mice.
31052177	0	1	from	Morphology	19:28	arg1	Disorders					80:88	the Autism Spectrum Disorders	60:88	the Autism Spectrum Disorders	60:88	Altered Intestinal Morphology and Microbiota Composition in the Autism Spectrum Disorders Associated SHANK3 Mouse Model.
31052177	8	2	theme	GI	1274:1275	arg1	tract					1277:1281	the GI tract	1270:1281	the GI tract that may contribute to the ASD phenotype by extracerebral mechanisms	1270:1350	We conclude that apart from its well-known role in the CNS, SHANK3 plays a specific role in the GI tract that may contribute to the ASD phenotype by extracerebral mechanisms.
31052177	6	3	theme	KO	904:905	arg1	mice					907:910	Shank3 KO mice	897:910	Shank3 KO mice that may contribute to inflammatory responses affecting brain development	897:984	Further, we detected a significantly altered microbiota composition measured in feces of Shank3 KO mice that may contribute to inflammatory responses affecting brain development.
31052177	6	4	theme	Shank3	897:902	arg1	mice					907:910	Shank3 KO mice	897:910	Shank3 KO mice that may contribute to inflammatory responses affecting brain development	897:984	Further, we detected a significantly altered microbiota composition measured in feces of Shank3 KO mice that may contribute to inflammatory responses affecting brain development.
31052177	0	5	theme	Mouse	108:112	arg1	Model					114:118	Mouse Model	108:118	Mouse Model	108:118	Altered Intestinal Morphology and Microbiota Composition in the Autism Spectrum Disorders Associated SHANK3 Mouse Model.
31052177	7	6	theme	mice	1086:1089	arg1	samples					1065:1071	liver samples	1059:1071	liver samples of Shank3 KO mice	1059:1089	In line with this, we found higher E. coli lipopolysaccharide levels in liver samples of Shank3 KO mice, and detected an increase in Interleukin-6 and activated astrocytes in Shank3 KO mice.
31052177	1	7	theme	repetitive	268:277	arg1	behaviors					279:287	repetitive behaviors	268:287	repetitive behaviors	268:287	Autism spectrum disorders (ASD) are a group of neurodevelopmental disorders characterized by deficits in social interaction and communication, and repetitive behaviors.
31052177	5	8	dep	gastrointestinal	693:708	arg1	GI					711:712	GI	711:712	GI	711:712	Here, we show expression in gastrointestinal (GI) epithelium and report a significantly different GI morphology in Shank3 knock-out (KO) mice.
31052177	7	9	theme	Shank3	1162:1167	arg1	mice					1172:1175	Shank3 KO mice	1162:1175	Shank3 KO mice	1162:1175	In line with this, we found higher E. coli lipopolysaccharide levels in liver samples of Shank3 KO mice, and detected an increase in Interleukin-6 and activated astrocytes in Shank3 KO mice.
31052177	7	10	theme	Shank3	1076:1081	arg1	mice					1086:1089	Shank3 KO mice	1076:1089	Shank3 KO mice	1076:1089	In line with this, we found higher E. coli lipopolysaccharide levels in liver samples of Shank3 KO mice, and detected an increase in Interleukin-6 and activated astrocytes in Shank3 KO mice.
31052177	5	11	theme	KO	798:799	arg1	mice					802:805	Shank3 knock-out (KO) mice	780:805	Shank3 knock-out (KO) mice	780:805	Here, we show expression in gastrointestinal (GI) epithelium and report a significantly different GI morphology in Shank3 knock-out (KO) mice.
31052177	3	12	theme	knock-out	534:542	arg1	models					550:555	Shank knock-out mouse models	528:555	Shank knock-out mouse models	528:555	Mutations and deletion of proteins of the SH3 and multiple ankyrin repeat domains (SHANK) gene-family were identified in patients with ASD, and Shank knock-out mouse models display autism-like phenotypes.
31052177	1	13	theme	neurodevelopmental	168:185	arg1	disorders					187:195	neurodevelopmental disorders	168:195	neurodevelopmental disorders characterized by deficits in social interaction and communication	168:261	Autism spectrum disorders (ASD) are a group of neurodevelopmental disorders characterized by deficits in social interaction and communication, and repetitive behaviors.
31052177	3	14	theme	domains	458:464	arg1	gene-family					474:484	the SH3 and multiple ankyrin repeat domains (SHANK) gene-family	422:484	the SH3 and multiple ankyrin repeat domains (SHANK) gene-family	422:484	Mutations and deletion of proteins of the SH3 and multiple ankyrin repeat domains (SHANK) gene-family were identified in patients with ASD, and Shank knock-out mouse models display autism-like phenotypes.
31052177	7	15	theme	KO	1169:1170	arg1	mice					1172:1175	Shank3 KO mice	1162:1175	Shank3 KO mice	1162:1175	In line with this, we found higher E. coli lipopolysaccharide levels in liver samples of Shank3 KO mice, and detected an increase in Interleukin-6 and activated astrocytes in Shank3 KO mice.
31052177	0	16	from	Composition	45:55	arg1	Disorders					80:88	the Autism Spectrum Disorders	60:88	the Autism Spectrum Disorders	60:88	Altered Intestinal Morphology and Microbiota Composition in the Autism Spectrum Disorders Associated SHANK3 Mouse Model.
31052177	7	17	theme	KO	1083:1084	arg1	mice					1086:1089	Shank3 KO mice	1076:1089	Shank3 KO mice	1076:1089	In line with this, we found higher E. coli lipopolysaccharide levels in liver samples of Shank3 KO mice, and detected an increase in Interleukin-6 and activated astrocytes in Shank3 KO mice.
31052177	3	18	theme	mouse	544:548	arg1	models					550:555	Shank knock-out mouse models	528:555	Shank knock-out mouse models	528:555	Mutations and deletion of proteins of the SH3 and multiple ankyrin repeat domains (SHANK) gene-family were identified in patients with ASD, and Shank knock-out mouse models display autism-like phenotypes.
31052177	0	19	theme	Intestinal	8:17	arg1	Morphology					19:28	Altered Intestinal Morphology	0:28	Altered Intestinal Morphology	0:28	Altered Intestinal Morphology and Microbiota Composition in the Autism Spectrum Disorders Associated SHANK3 Mouse Model.
31052177	3	20	with	patients	505:512	arg1	ASD					519:521	ASD	519:521	ASD	519:521	Mutations and deletion of proteins of the SH3 and multiple ankyrin repeat domains (SHANK) gene-family were identified in patients with ASD, and Shank knock-out mouse models display autism-like phenotypes.
31052177	2	21	theme	gastro-intestinal	326:342	arg1	problems					344:351	gastro-intestinal problems	326:351	gastro-intestinal problems	326:351	In addition, co-morbidities such as gastro-intestinal problems have frequently been reported.
31052177	3	22	theme	ankyrin	443:449	arg1	domains					458:464	the SH3 and multiple ankyrin repeat domains	422:464	domains	458:464	Mutations and deletion of proteins of the SH3 and multiple ankyrin repeat domains (SHANK) gene-family were identified in patients with ASD, and Shank knock-out mouse models display autism-like phenotypes.
31052177	0	23	theme	Altered	0:6	arg1	Morphology					19:28	Altered Intestinal Morphology	0:28	Altered Intestinal Morphology	0:28	Altered Intestinal Morphology and Microbiota Composition in the Autism Spectrum Disorders Associated SHANK3 Mouse Model.
31052177	6	24	theme	brain	968:972	arg1	development					974:984	brain development	968:984	brain development	968:984	Further, we detected a significantly altered microbiota composition measured in feces of Shank3 KO mice that may contribute to inflammatory responses affecting brain development.
31052177	3	25	theme	repeat	451:456	arg1	domains					458:464	the SH3 and multiple ankyrin repeat domains	422:464	domains	458:464	Mutations and deletion of proteins of the SH3 and multiple ankyrin repeat domains (SHANK) gene-family were identified in patients with ASD, and Shank knock-out mouse models display autism-like phenotypes.
31052177	5	26	theme	gastrointestinal	693:708	arg1	epithelium					715:724	gastrointestinal (GI) epithelium	693:724	gastrointestinal (GI) epithelium	693:724	Here, we show expression in gastrointestinal (GI) epithelium and report a significantly different GI morphology in Shank3 knock-out (KO) mice.
31052177	3	27	theme	autism-like	565:575	arg1	phenotypes					577:586	autism-like phenotypes	565:586	autism-like phenotypes	565:586	Mutations and deletion of proteins of the SH3 and multiple ankyrin repeat domains (SHANK) gene-family were identified in patients with ASD, and Shank knock-out mouse models display autism-like phenotypes.
31052177	3	28	theme	gene-family	474:484	arg1	proteins					410:417	proteins	410:417	proteins of the SH3 and multiple ankyrin repeat domains (SHANK) gene-family	410:484	Mutations and deletion of proteins of the SH3 and multiple ankyrin repeat domains (SHANK) gene-family were identified in patients with ASD, and Shank knock-out mouse models display autism-like phenotypes.
31052177	7	29	theme	activated	1138:1146	arg1	astrocytes					1148:1157	activated astrocytes	1138:1157	activated astrocytes	1138:1157	In line with this, we found higher E. coli lipopolysaccharide levels in liver samples of Shank3 KO mice, and detected an increase in Interleukin-6 and activated astrocytes in Shank3 KO mice.
31052177	4	30	theme	SHANK3	589:594	arg1	proteins					596:603	SHANK3 proteins	589:603	SHANK3 proteins	589:603	SHANK3 proteins are not only expressed in the central nervous system (CNS).
31052177	7	31	theme	liver	1059:1063	arg1	samples					1065:1071	liver samples	1059:1071	liver samples of Shank3 KO mice	1059:1089	In line with this, we found higher E. coli lipopolysaccharide levels in liver samples of Shank3 KO mice, and detected an increase in Interleukin-6 and activated astrocytes in Shank3 KO mice.
31052177	6	32	theme	microbiota	853:862	arg1	composition					864:874	a significantly altered microbiota composition	829:874	a significantly altered microbiota composition measured in feces of Shank3 KO mice that may contribute to inflammatory responses affecting brain development	829:984	Further, we detected a significantly altered microbiota composition measured in feces of Shank3 KO mice that may contribute to inflammatory responses affecting brain development.
31052177	8	33	theme	ASD	1310:1312	arg1	phenotype					1314:1322	the ASD phenotype	1306:1322	the ASD phenotype	1306:1322	We conclude that apart from its well-known role in the CNS, SHANK3 plays a specific role in the GI tract that may contribute to the ASD phenotype by extracerebral mechanisms.
31052177	4	34	theme	nervous	643:649	arg1	CNS					659:661	CNS	659:661	CNS	659:661	SHANK3 proteins are not only expressed in the central nervous system (CNS).
31052177	4	34	theme	nervous	643:649	arg1	system					651:656	the central nervous system	631:656	the central nervous system (CNS)	631:662	SHANK3 proteins are not only expressed in the central nervous system (CNS).
31052177	6	35	theme	inflammatory	935:946	arg1	responses					948:956	inflammatory responses	935:956	inflammatory responses affecting brain development	935:984	Further, we detected a significantly altered microbiota composition measured in feces of Shank3 KO mice that may contribute to inflammatory responses affecting brain development.
31052177	7	36	theme	lipopolysaccharide	1030:1047	arg1	levels					1049:1054	higher E. coli lipopolysaccharide levels	1015:1054	higher E. coli lipopolysaccharide levels	1015:1054	In line with this, we found higher E. coli lipopolysaccharide levels in liver samples of Shank3 KO mice, and detected an increase in Interleukin-6 and activated astrocytes in Shank3 KO mice.
31052177	6	37	theme	altered	845:851	arg1	composition					864:874	a significantly altered microbiota composition	829:874	a significantly altered microbiota composition measured in feces of Shank3 KO mice that may contribute to inflammatory responses affecting brain development	829:984	Further, we detected a significantly altered microbiota composition measured in feces of Shank3 KO mice that may contribute to inflammatory responses affecting brain development.
31052177	8	38	theme	extracerebral	1327:1339	arg1	mechanisms					1341:1350	extracerebral mechanisms	1327:1350	extracerebral mechanisms	1327:1350	We conclude that apart from its well-known role in the CNS, SHANK3 plays a specific role in the GI tract that may contribute to the ASD phenotype by extracerebral mechanisms.
31052177	1	39	theme	disorders	187:195	arg1	disorders					137:145	Autism spectrum disorders	121:145	Autism spectrum disorders (ASD)	121:151	Autism spectrum disorders (ASD) are a group of neurodevelopmental disorders characterized by deficits in social interaction and communication, and repetitive behaviors.
31052177	1	39	theme	disorders	187:195	arg1	group					159:163	a group	157:163	a group of neurodevelopmental disorders characterized by deficits in social interaction and communication	157:261	Autism spectrum disorders (ASD) are a group of neurodevelopmental disorders characterized by deficits in social interaction and communication, and repetitive behaviors.
31052177	1	39	theme	disorders	187:195	arg1	disorders					187:195	neurodevelopmental disorders	168:195	neurodevelopmental disorders characterized by deficits in social interaction and communication	168:261	Autism spectrum disorders (ASD) are a group of neurodevelopmental disorders characterized by deficits in social interaction and communication, and repetitive behaviors.
31052177	1	39	theme	disorders	187:195	arg1	behaviors					279:287	repetitive behaviors	268:287	repetitive behaviors	268:287	Autism spectrum disorders (ASD) are a group of neurodevelopmental disorders characterized by deficits in social interaction and communication, and repetitive behaviors.
31052177	0	40	theme	Microbiota	34:43	arg1	Composition					45:55	Microbiota Composition	34:55	Microbiota Composition	34:55	Altered Intestinal Morphology and Microbiota Composition in the Autism Spectrum Disorders Associated SHANK3 Mouse Model.
31052177	3	41	theme	multiple	434:441	arg1	domains					458:464	the SH3 and multiple ankyrin repeat domains	422:464	domains	458:464	Mutations and deletion of proteins of the SH3 and multiple ankyrin repeat domains (SHANK) gene-family were identified in patients with ASD, and Shank knock-out mouse models display autism-like phenotypes.
31052177	1	42	from	deficits	214:221	arg1	communication					249:261	communication	249:261	communication	249:261	Autism spectrum disorders (ASD) are a group of neurodevelopmental disorders characterized by deficits in social interaction and communication, and repetitive behaviors.
31052177	1	42	from	deficits	214:221	arg1	interaction					233:243	social interaction	226:243	social interaction	226:243	Autism spectrum disorders (ASD) are a group of neurodevelopmental disorders characterized by deficits in social interaction and communication, and repetitive behaviors.
31052177	5	43	theme	knock-out	787:795	arg1	mice					802:805	Shank3 knock-out (KO) mice	780:805	Shank3 knock-out (KO) mice	780:805	Here, we show expression in gastrointestinal (GI) epithelium and report a significantly different GI morphology in Shank3 knock-out (KO) mice.
31052177	7	44	theme	E.	1022:1023	arg1	lipopolysaccharide					1030:1047	E. coli lipopolysaccharide	1022:1047	higher E. coli lipopolysaccharide levels	1015:1054	In line with this, we found higher E. coli lipopolysaccharide levels in liver samples of Shank3 KO mice, and detected an increase in Interleukin-6 and activated astrocytes in Shank3 KO mice.
31052177	5	45	theme	different	753:761	arg1	morphology					766:775	a significantly different GI morphology	737:775	a significantly different GI morphology in Shank3 knock-out (KO) mice	737:805	Here, we show expression in gastrointestinal (GI) epithelium and report a significantly different GI morphology in Shank3 knock-out (KO) mice.
31052177	8	46	theme	well-known	1210:1219	arg1	role					1221:1224	its well-known role	1206:1224	its well-known role in the CNS	1206:1235	We conclude that apart from its well-known role in the CNS, SHANK3 plays a specific role in the GI tract that may contribute to the ASD phenotype by extracerebral mechanisms.
31052177	7	47	from	astrocytes	1148:1157	arg1	Interleukin-6					1120:1132	Interleukin-6	1120:1132	Interleukin-6	1120:1132	In line with this, we found higher E. coli lipopolysaccharide levels in liver samples of Shank3 KO mice, and detected an increase in Interleukin-6 and activated astrocytes in Shank3 KO mice.
31052177	1	48	theme	Autism	121:126	arg1	disorders					137:145	Autism spectrum disorders	121:145	Autism spectrum disorders (ASD)	121:151	Autism spectrum disorders (ASD) are a group of neurodevelopmental disorders characterized by deficits in social interaction and communication, and repetitive behaviors.
31052177	1	48	theme	Autism	121:126	arg1	group					159:163	a group	157:163	a group of neurodevelopmental disorders characterized by deficits in social interaction and communication	157:261	Autism spectrum disorders (ASD) are a group of neurodevelopmental disorders characterized by deficits in social interaction and communication, and repetitive behaviors.
31052177	1	48	theme	Autism	121:126	arg1	disorders					187:195	neurodevelopmental disorders	168:195	neurodevelopmental disorders characterized by deficits in social interaction and communication	168:261	Autism spectrum disorders (ASD) are a group of neurodevelopmental disorders characterized by deficits in social interaction and communication, and repetitive behaviors.
31052177	1	48	theme	Autism	121:126	arg1	ASD					148:150	ASD	148:150	ASD	148:150	Autism spectrum disorders (ASD) are a group of neurodevelopmental disorders characterized by deficits in social interaction and communication, and repetitive behaviors.
31052177	3	49	theme	SH3	426:428	arg1	gene-family					474:484	the SH3 and multiple ankyrin repeat domains (SHANK) gene-family	422:484	the SH3 and multiple ankyrin repeat domains (SHANK) gene-family	422:484	Mutations and deletion of proteins of the SH3 and multiple ankyrin repeat domains (SHANK) gene-family were identified in patients with ASD, and Shank knock-out mouse models display autism-like phenotypes.
31052177	7	50	theme	coli	1025:1028	arg1	lipopolysaccharide					1030:1047	E. coli lipopolysaccharide	1022:1047	higher E. coli lipopolysaccharide levels	1015:1054	In line with this, we found higher E. coli lipopolysaccharide levels in liver samples of Shank3 KO mice, and detected an increase in Interleukin-6 and activated astrocytes in Shank3 KO mice.
31052177	1	51	theme	spectrum	128:135	arg1	disorders					137:145	Autism spectrum disorders	121:145	Autism spectrum disorders (ASD)	121:151	Autism spectrum disorders (ASD) are a group of neurodevelopmental disorders characterized by deficits in social interaction and communication, and repetitive behaviors.
31052177	1	51	theme	spectrum	128:135	arg1	group					159:163	a group	157:163	a group of neurodevelopmental disorders characterized by deficits in social interaction and communication	157:261	Autism spectrum disorders (ASD) are a group of neurodevelopmental disorders characterized by deficits in social interaction and communication, and repetitive behaviors.
31052177	1	51	theme	spectrum	128:135	arg1	disorders					187:195	neurodevelopmental disorders	168:195	neurodevelopmental disorders characterized by deficits in social interaction and communication	168:261	Autism spectrum disorders (ASD) are a group of neurodevelopmental disorders characterized by deficits in social interaction and communication, and repetitive behaviors.
31052177	1	51	theme	spectrum	128:135	arg1	ASD					148:150	ASD	148:150	ASD	148:150	Autism spectrum disorders (ASD) are a group of neurodevelopmental disorders characterized by deficits in social interaction and communication, and repetitive behaviors.
31052177	5	52	from	morphology	766:775	arg1	mice					802:805	Shank3 knock-out (KO) mice	780:805	Shank3 knock-out (KO) mice	780:805	Here, we show expression in gastrointestinal (GI) epithelium and report a significantly different GI morphology in Shank3 knock-out (KO) mice.
31052177	7	53	from	increase	1108:1115	arg1	Interleukin-6					1120:1132	Interleukin-6	1120:1132	Interleukin-6	1120:1132	In line with this, we found higher E. coli lipopolysaccharide levels in liver samples of Shank3 KO mice, and detected an increase in Interleukin-6 and activated astrocytes in Shank3 KO mice.
31052177	5	54	from	expression	679:688	arg1	epithelium					715:724	gastrointestinal (GI) epithelium	693:724	gastrointestinal (GI) epithelium	693:724	Here, we show expression in gastrointestinal (GI) epithelium and report a significantly different GI morphology in Shank3 knock-out (KO) mice.
31052177	1	55	theme	social	226:231	arg1	interaction					233:243	social interaction	226:243	social interaction	226:243	Autism spectrum disorders (ASD) are a group of neurodevelopmental disorders characterized by deficits in social interaction and communication, and repetitive behaviors.
31052177	7	56	theme	higher	1015:1020	arg1	levels					1049:1054	higher E. coli lipopolysaccharide levels	1015:1054	higher E. coli lipopolysaccharide levels	1015:1054	In line with this, we found higher E. coli lipopolysaccharide levels in liver samples of Shank3 KO mice, and detected an increase in Interleukin-6 and activated astrocytes in Shank3 KO mice.
31052177	8	57	from	role	1221:1224	arg1	CNS					1233:1235	the CNS	1229:1235	the CNS	1229:1235	We conclude that apart from its well-known role in the CNS, SHANK3 plays a specific role in the GI tract that may contribute to the ASD phenotype by extracerebral mechanisms.
31052177	8	58	theme	specific	1253:1260	arg1	role					1262:1265	a specific role	1251:1265	a specific role	1251:1265	We conclude that apart from its well-known role in the CNS, SHANK3 plays a specific role in the GI tract that may contribute to the ASD phenotype by extracerebral mechanisms.
31052177	0	59	theme	Spectrum	71:78	arg1	Disorders					80:88	the Autism Spectrum Disorders	60:88	the Autism Spectrum Disorders	60:88	Altered Intestinal Morphology and Microbiota Composition in the Autism Spectrum Disorders Associated SHANK3 Mouse Model.
31052177	3	60	theme	proteins	410:417	arg1	deletion					398:405	deletion	398:405	deletion of proteins of the SH3 and multiple ankyrin repeat domains (SHANK) gene-family	398:484	Mutations and deletion of proteins of the SH3 and multiple ankyrin repeat domains (SHANK) gene-family were identified in patients with ASD, and Shank knock-out mouse models display autism-like phenotypes.
31052177	3	60	theme	proteins	410:417	arg1	Mutations					384:392	Mutations	384:392	Mutations	384:392	Mutations and deletion of proteins of the SH3 and multiple ankyrin repeat domains (SHANK) gene-family were identified in patients with ASD, and Shank knock-out mouse models display autism-like phenotypes.
31052177	4	61	theme	central	635:641	arg1	CNS					659:661	CNS	659:661	CNS	659:661	SHANK3 proteins are not only expressed in the central nervous system (CNS).
31052177	4	61	theme	central	635:641	arg1	system					651:656	the central nervous system	631:656	the central nervous system (CNS)	631:662	SHANK3 proteins are not only expressed in the central nervous system (CNS).
31052177	0	62	theme	Autism	64:69	arg1	Disorders					80:88	the Autism Spectrum Disorders	60:88	the Autism Spectrum Disorders	60:88	Altered Intestinal Morphology and Microbiota Composition in the Autism Spectrum Disorders Associated SHANK3 Mouse Model.
31052177	6	63	theme	mice	907:910	arg1	feces					888:892	feces	888:892	feces of Shank3 KO mice that may contribute to inflammatory responses affecting brain development	888:984	Further, we detected a significantly altered microbiota composition measured in feces of Shank3 KO mice that may contribute to inflammatory responses affecting brain development.
31052177	5	64	theme	Shank3	780:785	arg1	mice					802:805	Shank3 knock-out (KO) mice	780:805	Shank3 knock-out (KO) mice	780:805	Here, we show expression in gastrointestinal (GI) epithelium and report a significantly different GI morphology in Shank3 knock-out (KO) mice.
31052177	3	65	theme	Shank	528:532	arg1	models					550:555	Shank knock-out mouse models	528:555	Shank knock-out mouse models	528:555	Mutations and deletion of proteins of the SH3 and multiple ankyrin repeat domains (SHANK) gene-family were identified in patients with ASD, and Shank knock-out mouse models display autism-like phenotypes.
31052177	7	66	with	line	990:993	arg1	this					1000:1003	this	1000:1003	this	1000:1003	In line with this, we found higher E. coli lipopolysaccharide levels in liver samples of Shank3 KO mice, and detected an increase in Interleukin-6 and activated astrocytes in Shank3 KO mice.
31661298	0	0	theme	lipid	87:91	arg1	content					93:99	liver lipid content	81:99	liver lipid content	81:99	Hepatic IKKε expression is dispensable for high-fat feeding-induced increases in liver lipid content and alterations in glucose tolerance.
31661298	4	1	theme	whole	844:848	arg1	tolerance					863:871	whole body glucose tolerance	844:871	whole body glucose tolerance	844:871	IKKεAlb-/- mice and littermate controls gain weight, show similar changes in body composition, and do not display any improvements in insulin sensitivity or whole body glucose tolerance.
31661298	4	2	from	improvements	805:816	arg1	tolerance					863:871	whole body glucose tolerance	844:871	whole body glucose tolerance	844:871	IKKεAlb-/- mice and littermate controls gain weight, show similar changes in body composition, and do not display any improvements in insulin sensitivity or whole body glucose tolerance.
31661298	4	2	from	improvements	805:816	arg1	sensitivity					829:839	insulin sensitivity	821:839	insulin sensitivity	821:839	IKKεAlb-/- mice and littermate controls gain weight, show similar changes in body composition, and do not display any improvements in insulin sensitivity or whole body glucose tolerance.
31661298	2	3	theme	protein	353:359	arg1	kinase					361:366	an inducible protein kinase	340:366	an inducible protein kinase	340:366	The inhibitor of nuclear factor-κB kinase-ε (IKKε) was originally described as an inducible protein kinase; whole body gene deletion or systemic pharmaceutical targeting of this kinase improved insulin sensitivity and glucose tolerance in mice.
31661298	2	3	theme	protein	353:359	arg1	inhibitor					265:273	The inhibitor	261:273	The inhibitor of nuclear factor-κB kinase-ε (IKKε)	261:310	The inhibitor of nuclear factor-κB kinase-ε (IKKε) was originally described as an inducible protein kinase; whole body gene deletion or systemic pharmaceutical targeting of this kinase improved insulin sensitivity and glucose tolerance in mice.
31661298	1	4	theme	states	253:258	arg1	progression					213:223	progression	213:223	progression	213:223	There are endocrine and immunological changes that occur during onset and progression of the overweight and obese states.
31661298	1	4	theme	states	253:258	arg1	onset					203:207	onset	203:207	onset	203:207	There are endocrine and immunological changes that occur during onset and progression of the overweight and obese states.
31661298	0	5	theme	liver	81:85	arg1	content					93:99	liver lipid content	81:99	liver lipid content	81:99	Hepatic IKKε expression is dispensable for high-fat feeding-induced increases in liver lipid content and alterations in glucose tolerance.
31661298	1	6	theme	immunological	163:175	arg1	changes					177:183	endocrine and immunological changes	149:183	endocrine and immunological changes that occur during onset and progression of the overweight and obese states	149:258	There are endocrine and immunological changes that occur during onset and progression of the overweight and obese states.
31661298	6	7	theme	glycogen	971:978	arg1	accumulation					980:991	glycogen accumulation	971:991	glycogen accumulation in the liver	971:1004	While glycogen accumulation in the liver is reduced in IKKεAlb-/- mice, lipid storage in liver is similar in IKKεAlb-/- mice and littermate controls.
31661298	7	8	theme	primary	1166:1172	arg1	action					1174:1179	the primary action	1162:1179	the primary action of this kinase to impact insulin sensitivity during weight gain	1162:1243	Our results using IKKεAlb-/- mice suggest that the primary action of this kinase to impact insulin sensitivity during weight gain lies predominantly within extrahepatic tissues.
31661298	4	9	theme	body	764:767	arg1	composition					769:779	body composition	764:779	body composition	764:779	IKKεAlb-/- mice and littermate controls gain weight, show similar changes in body composition, and do not display any improvements in insulin sensitivity or whole body glucose tolerance.
31661298	4	10	theme	glucose	855:861	arg1	tolerance					863:871	whole body glucose tolerance	844:871	whole body glucose tolerance	844:871	IKKεAlb-/- mice and littermate controls gain weight, show similar changes in body composition, and do not display any improvements in insulin sensitivity or whole body glucose tolerance.
31661298	4	11	from	changes	753:759	arg1	composition					769:779	body composition	764:779	body composition	764:779	IKKεAlb-/- mice and littermate controls gain weight, show similar changes in body composition, and do not display any improvements in insulin sensitivity or whole body glucose tolerance.
31661298	2	12	theme	kinase-ε	296:303	arg1	kinase					361:366	an inducible protein kinase	340:366	an inducible protein kinase	340:366	The inhibitor of nuclear factor-κB kinase-ε (IKKε) was originally described as an inducible protein kinase; whole body gene deletion or systemic pharmaceutical targeting of this kinase improved insulin sensitivity and glucose tolerance in mice.
31661298	2	12	theme	kinase-ε	296:303	arg1	inhibitor					265:273	The inhibitor	261:273	The inhibitor of nuclear factor-κB kinase-ε (IKKε)	261:310	The inhibitor of nuclear factor-κB kinase-ε (IKKε) was originally described as an inducible protein kinase; whole body gene deletion or systemic pharmaceutical targeting of this kinase improved insulin sensitivity and glucose tolerance in mice.
31661298	5	13	theme	matched	932:938	arg1	diets					958:962	matched low- vs. high-fat diets	932:962	matched low- vs. high-fat diets	932:962	These studies were conducted using breeder chow diets and matched low- vs. high-fat diets.
31661298	7	14	theme	weight	1233:1238	arg1	gain					1240:1243	weight gain	1233:1243	weight gain	1233:1243	Our results using IKKεAlb-/- mice suggest that the primary action of this kinase to impact insulin sensitivity during weight gain lies predominantly within extrahepatic tissues.
31661298	4	15	theme	body	850:853	arg1	tolerance					863:871	whole body glucose tolerance	844:871	whole body glucose tolerance	844:871	IKKεAlb-/- mice and littermate controls gain weight, show similar changes in body composition, and do not display any improvements in insulin sensitivity or whole body glucose tolerance.
31661298	7	16	theme	insulin	1206:1212	arg1	sensitivity					1214:1224	impact insulin sensitivity	1199:1224	impact insulin sensitivity	1199:1224	Our results using IKKεAlb-/- mice suggest that the primary action of this kinase to impact insulin sensitivity during weight gain lies predominantly within extrahepatic tissues.
31661298	2	17	theme	factor-κB	286:294	arg1	IKKε					306:309	IKKε	306:309	IKKε	306:309	The inhibitor of nuclear factor-κB kinase-ε (IKKε) was originally described as an inducible protein kinase; whole body gene deletion or systemic pharmaceutical targeting of this kinase improved insulin sensitivity and glucose tolerance in mice.
31661298	2	17	theme	factor-κB	286:294	arg1	kinase-ε					296:303	nuclear factor-κB kinase-ε	278:303	nuclear factor-κB kinase-ε (IKKε)	278:310	The inhibitor of nuclear factor-κB kinase-ε (IKKε) was originally described as an inducible protein kinase; whole body gene deletion or systemic pharmaceutical targeting of this kinase improved insulin sensitivity and glucose tolerance in mice.
31661298	5	18	theme	breeder	909:915	arg1	chow					917:920	breeder chow diets and matched low- vs. high-fat diets	909:962	chow	917:920	These studies were conducted using breeder chow diets and matched low- vs. high-fat diets.
31661298	3	19	from	elimination	640:650	arg1	IKKεAlb-/-					674:683	IKKεAlb-/-	674:683	IKKεAlb-/-	674:683	To investigate the primary sites of action associated with IKKε during weight gain, we describe the first mouse line with conditional elimination of IKKε in the liver (IKKεAlb-/-).
31661298	3	19	from	elimination	640:650	arg1	liver					667:671	the liver	663:671	the liver (IKKεAlb-/-)	663:684	To investigate the primary sites of action associated with IKKε during weight gain, we describe the first mouse line with conditional elimination of IKKε in the liver (IKKεAlb-/-).
31661298	7	20	theme	IKKεAlb-/-	1133:1142	arg1	mice					1144:1147	IKKεAlb-/- mice	1133:1147	IKKεAlb-/- mice	1133:1147	Our results using IKKεAlb-/- mice suggest that the primary action of this kinase to impact insulin sensitivity during weight gain lies predominantly within extrahepatic tissues.
31661298	6	21	theme	IKKεAlb-/-	1020:1029	arg1	mice					1031:1034	IKKεAlb-/- mice	1020:1034	IKKεAlb-/- mice	1020:1034	While glycogen accumulation in the liver is reduced in IKKεAlb-/- mice, lipid storage in liver is similar in IKKεAlb-/- mice and littermate controls.
31661298	2	22	theme	nuclear	278:284	arg1	IKKε					306:309	IKKε	306:309	IKKε	306:309	The inhibitor of nuclear factor-κB kinase-ε (IKKε) was originally described as an inducible protein kinase; whole body gene deletion or systemic pharmaceutical targeting of this kinase improved insulin sensitivity and glucose tolerance in mice.
31661298	2	22	theme	nuclear	278:284	arg1	kinase-ε					296:303	nuclear factor-κB kinase-ε	278:303	nuclear factor-κB kinase-ε (IKKε)	278:310	The inhibitor of nuclear factor-κB kinase-ε (IKKε) was originally described as an inducible protein kinase; whole body gene deletion or systemic pharmaceutical targeting of this kinase improved insulin sensitivity and glucose tolerance in mice.
31661298	6	23	theme	lipid	1037:1041	arg1	storage					1043:1049	lipid storage	1037:1049	lipid storage in liver	1037:1058	While glycogen accumulation in the liver is reduced in IKKεAlb-/- mice, lipid storage in liver is similar in IKKεAlb-/- mice and littermate controls.
31661298	5	24	dep	chow	917:920	arg1	diets					922:926	diets	922:926	diets	922:926	These studies were conducted using breeder chow diets and matched low- vs. high-fat diets.
31661298	0	25	theme	high-fat	43:50	arg1	increases					68:76	high-fat feeding-induced increases	43:76	high-fat feeding-induced increases in liver lipid content and alterations in glucose tolerance	43:136	Hepatic IKKε expression is dispensable for high-fat feeding-induced increases in liver lipid content and alterations in glucose tolerance.
31661298	2	26	theme	kinase	439:444	arg1	deletion					385:392	whole body gene deletion	369:392	whole body gene deletion	369:392	The inhibitor of nuclear factor-κB kinase-ε (IKKε) was originally described as an inducible protein kinase; whole body gene deletion or systemic pharmaceutical targeting of this kinase improved insulin sensitivity and glucose tolerance in mice.
31661298	2	26	theme	kinase	439:444	arg1	targeting					421:429	systemic pharmaceutical targeting	397:429	systemic pharmaceutical targeting	397:429	The inhibitor of nuclear factor-κB kinase-ε (IKKε) was originally described as an inducible protein kinase; whole body gene deletion or systemic pharmaceutical targeting of this kinase improved insulin sensitivity and glucose tolerance in mice.
31661298	5	27	theme	high-fat	949:956	arg1	diets					958:962	matched low- vs. high-fat diets	932:962	matched low- vs. high-fat diets	932:962	These studies were conducted using breeder chow diets and matched low- vs. high-fat diets.
31661298	2	28	theme	gene	380:383	arg1	deletion					385:392	whole body gene deletion	369:392	whole body gene deletion	369:392	The inhibitor of nuclear factor-κB kinase-ε (IKKε) was originally described as an inducible protein kinase; whole body gene deletion or systemic pharmaceutical targeting of this kinase improved insulin sensitivity and glucose tolerance in mice.
31661298	0	29	theme	IKKε	8:11	arg1	expression					13:22	Hepatic IKKε expression	0:22	Hepatic IKKε expression	0:22	Hepatic IKKε expression is dispensable for high-fat feeding-induced increases in liver lipid content and alterations in glucose tolerance.
31661298	3	30	with	line	618:621	arg1	elimination					640:650	conditional elimination	628:650	conditional elimination of IKKε in the liver (IKKεAlb-/-)	628:684	To investigate the primary sites of action associated with IKKε during weight gain, we describe the first mouse line with conditional elimination of IKKε in the liver (IKKεAlb-/-).
31661298	6	31	from	accumulation	980:991	arg1	liver					1000:1004	the liver	996:1004	the liver	996:1004	While glycogen accumulation in the liver is reduced in IKKεAlb-/- mice, lipid storage in liver is similar in IKKεAlb-/- mice and littermate controls.
31661298	2	32	theme	body	375:378	arg1	deletion					385:392	whole body gene deletion	369:392	whole body gene deletion	369:392	The inhibitor of nuclear factor-κB kinase-ε (IKKε) was originally described as an inducible protein kinase; whole body gene deletion or systemic pharmaceutical targeting of this kinase improved insulin sensitivity and glucose tolerance in mice.
31661298	0	33	theme	Hepatic	0:6	arg1	expression					13:22	Hepatic IKKε expression	0:22	Hepatic IKKε expression	0:22	Hepatic IKKε expression is dispensable for high-fat feeding-induced increases in liver lipid content and alterations in glucose tolerance.
31661298	4	34	theme	insulin	821:827	arg1	sensitivity					829:839	insulin sensitivity	821:839	insulin sensitivity	821:839	IKKεAlb-/- mice and littermate controls gain weight, show similar changes in body composition, and do not display any improvements in insulin sensitivity or whole body glucose tolerance.
31661298	3	35	theme	weight	577:582	arg1	gain					584:587	weight gain	577:587	weight gain	577:587	To investigate the primary sites of action associated with IKKε during weight gain, we describe the first mouse line with conditional elimination of IKKε in the liver (IKKεAlb-/-).
31661298	4	36	theme	similar	745:751	arg1	changes					753:759	similar changes	745:759	similar changes in body composition	745:779	IKKεAlb-/- mice and littermate controls gain weight, show similar changes in body composition, and do not display any improvements in insulin sensitivity or whole body glucose tolerance.
31661298	2	37	theme	whole	369:373	arg1	deletion					385:392	whole body gene deletion	369:392	whole body gene deletion	369:392	The inhibitor of nuclear factor-κB kinase-ε (IKKε) was originally described as an inducible protein kinase; whole body gene deletion or systemic pharmaceutical targeting of this kinase improved insulin sensitivity and glucose tolerance in mice.
31661298	6	38	from	storage	1043:1049	arg1	liver					1054:1058	liver	1054:1058	liver	1054:1058	While glycogen accumulation in the liver is reduced in IKKεAlb-/- mice, lipid storage in liver is similar in IKKεAlb-/- mice and littermate controls.
31661298	6	39	from	controls	1105:1112	arg1	similar					1063:1069	similar	1063:1069	similar	1063:1069	While glycogen accumulation in the liver is reduced in IKKεAlb-/- mice, lipid storage in liver is similar in IKKεAlb-/- mice and littermate controls.
31661298	5	40	theme	low-	940:943	arg1	diets					958:962	matched low- vs. high-fat diets	932:962	matched low- vs. high-fat diets	932:962	These studies were conducted using breeder chow diets and matched low- vs. high-fat diets.
31661298	6	41	theme	IKKεAlb-/-	1074:1083	arg1	mice					1085:1088	IKKεAlb-/- mice	1074:1088	IKKεAlb-/- mice	1074:1088	While glycogen accumulation in the liver is reduced in IKKεAlb-/- mice, lipid storage in liver is similar in IKKεAlb-/- mice and littermate controls.
31661298	0	42	theme	glucose	120:126	arg1	tolerance					128:136	glucose tolerance	120:136	glucose tolerance	120:136	Hepatic IKKε expression is dispensable for high-fat feeding-induced increases in liver lipid content and alterations in glucose tolerance.
31661298	2	43	theme	inducible	343:351	arg1	kinase					361:366	an inducible protein kinase	340:366	an inducible protein kinase	340:366	The inhibitor of nuclear factor-κB kinase-ε (IKKε) was originally described as an inducible protein kinase; whole body gene deletion or systemic pharmaceutical targeting of this kinase improved insulin sensitivity and glucose tolerance in mice.
31661298	2	43	theme	inducible	343:351	arg1	inhibitor					265:273	The inhibitor	261:273	The inhibitor of nuclear factor-κB kinase-ε (IKKε)	261:310	The inhibitor of nuclear factor-κB kinase-ε (IKKε) was originally described as an inducible protein kinase; whole body gene deletion or systemic pharmaceutical targeting of this kinase improved insulin sensitivity and glucose tolerance in mice.
31661298	2	44	theme	pharmaceutical	406:419	arg1	targeting					421:429	systemic pharmaceutical targeting	397:429	systemic pharmaceutical targeting	397:429	The inhibitor of nuclear factor-κB kinase-ε (IKKε) was originally described as an inducible protein kinase; whole body gene deletion or systemic pharmaceutical targeting of this kinase improved insulin sensitivity and glucose tolerance in mice.
31661298	2	45	theme	glucose	479:485	arg1	tolerance					487:495	glucose tolerance	479:495	glucose tolerance	479:495	The inhibitor of nuclear factor-κB kinase-ε (IKKε) was originally described as an inducible protein kinase; whole body gene deletion or systemic pharmaceutical targeting of this kinase improved insulin sensitivity and glucose tolerance in mice.
31661298	6	46	from	similar	1063:1069	arg1	mice					1085:1088	IKKεAlb-/- mice	1074:1088	IKKεAlb-/- mice	1074:1088	While glycogen accumulation in the liver is reduced in IKKεAlb-/- mice, lipid storage in liver is similar in IKKεAlb-/- mice and littermate controls.
31661298	6	46	from	similar	1063:1069	arg1	controls					1105:1112	littermate controls	1094:1112	littermate controls	1094:1112	While glycogen accumulation in the liver is reduced in IKKεAlb-/- mice, lipid storage in liver is similar in IKKεAlb-/- mice and littermate controls.
31661298	2	47	theme	systemic	397:404	arg1	targeting					421:429	systemic pharmaceutical targeting	397:429	systemic pharmaceutical targeting	397:429	The inhibitor of nuclear factor-κB kinase-ε (IKKε) was originally described as an inducible protein kinase; whole body gene deletion or systemic pharmaceutical targeting of this kinase improved insulin sensitivity and glucose tolerance in mice.
31661298	6	48	from	mice	1085:1088	arg1	similar					1063:1069	similar	1063:1069	similar	1063:1069	While glycogen accumulation in the liver is reduced in IKKεAlb-/- mice, lipid storage in liver is similar in IKKεAlb-/- mice and littermate controls.
31661298	7	49	theme	extrahepatic	1271:1282	arg1	tissues					1284:1290	extrahepatic tissues	1271:1290	extrahepatic tissues	1271:1290	Our results using IKKεAlb-/- mice suggest that the primary action of this kinase to impact insulin sensitivity during weight gain lies predominantly within extrahepatic tissues.
31661298	0	50	theme	feeding-induced	52:66	arg1	increases					68:76	high-fat feeding-induced increases	43:76	high-fat feeding-induced increases in liver lipid content and alterations in glucose tolerance	43:136	Hepatic IKKε expression is dispensable for high-fat feeding-induced increases in liver lipid content and alterations in glucose tolerance.
31661298	0	51	from	increases	68:76	arg1	alterations					105:115	alterations	105:115	alterations in glucose tolerance	105:136	Hepatic IKKε expression is dispensable for high-fat feeding-induced increases in liver lipid content and alterations in glucose tolerance.
31661298	0	51	from	increases	68:76	arg1	content					93:99	liver lipid content	81:99	liver lipid content	81:99	Hepatic IKKε expression is dispensable for high-fat feeding-induced increases in liver lipid content and alterations in glucose tolerance.
31661298	6	52	theme	littermate	1094:1103	arg1	controls					1105:1112	littermate controls	1094:1112	littermate controls	1094:1112	While glycogen accumulation in the liver is reduced in IKKεAlb-/- mice, lipid storage in liver is similar in IKKεAlb-/- mice and littermate controls.
31661298	2	53	theme	insulin	455:461	arg1	sensitivity					463:473	insulin sensitivity	455:473	insulin sensitivity	455:473	The inhibitor of nuclear factor-κB kinase-ε (IKKε) was originally described as an inducible protein kinase; whole body gene deletion or systemic pharmaceutical targeting of this kinase improved insulin sensitivity and glucose tolerance in mice.
31661298	3	54	theme	conditional	628:638	arg1	elimination					640:650	conditional elimination	628:650	conditional elimination of IKKε in the liver (IKKεAlb-/-)	628:684	To investigate the primary sites of action associated with IKKε during weight gain, we describe the first mouse line with conditional elimination of IKKε in the liver (IKKεAlb-/-).
31661298	4	55	theme	IKKεAlb-/-	687:696	arg1	mice					698:701	IKKεAlb-/- mice	687:701	IKKεAlb-/- mice	687:701	IKKεAlb-/- mice and littermate controls gain weight, show similar changes in body composition, and do not display any improvements in insulin sensitivity or whole body glucose tolerance.
31661298	0	56	from	alterations	105:115	arg1	tolerance					128:136	glucose tolerance	120:136	glucose tolerance	120:136	Hepatic IKKε expression is dispensable for high-fat feeding-induced increases in liver lipid content and alterations in glucose tolerance.
31661298	3	57	theme	mouse	612:616	arg1	line					618:621	the first mouse line	602:621	the first mouse line with conditional elimination of IKKε in the liver (IKKεAlb-/-)	602:684	To investigate the primary sites of action associated with IKKε during weight gain, we describe the first mouse line with conditional elimination of IKKε in the liver (IKKεAlb-/-).
31661298	4	58	theme	littermate	707:716	arg1	controls					718:725	littermate controls	707:725	littermate controls	707:725	IKKεAlb-/- mice and littermate controls gain weight, show similar changes in body composition, and do not display any improvements in insulin sensitivity or whole body glucose tolerance.
31661298	3	59	theme	IKKε	655:658	arg1	elimination					640:650	conditional elimination	628:650	conditional elimination of IKKε in the liver (IKKεAlb-/-)	628:684	To investigate the primary sites of action associated with IKKε during weight gain, we describe the first mouse line with conditional elimination of IKKε in the liver (IKKεAlb-/-).
31661298	3	60	theme	action	542:547	arg1	action					542:547	action	542:547	action associated with IKKε during weight gain	542:587	To investigate the primary sites of action associated with IKKε during weight gain, we describe the first mouse line with conditional elimination of IKKε in the liver (IKKεAlb-/-).
31661298	3	60	theme	action	542:547	arg1	sites					533:537	the primary sites	521:537	the primary sites of action associated with IKKε during weight gain	521:587	To investigate the primary sites of action associated with IKKε during weight gain, we describe the first mouse line with conditional elimination of IKKε in the liver (IKKεAlb-/-).
31661298	3	61	theme	primary	525:531	arg1	action					542:547	action	542:547	action associated with IKKε during weight gain	542:587	To investigate the primary sites of action associated with IKKε during weight gain, we describe the first mouse line with conditional elimination of IKKε in the liver (IKKεAlb-/-).
31661298	3	61	theme	primary	525:531	arg1	sites					533:537	the primary sites	521:537	the primary sites of action associated with IKKε during weight gain	521:587	To investigate the primary sites of action associated with IKKε during weight gain, we describe the first mouse line with conditional elimination of IKKε in the liver (IKKεAlb-/-).
31661298	1	62	theme	overweight	232:241	arg1	states					253:258	the overweight and obese states	228:258	the overweight and obese states	228:258	There are endocrine and immunological changes that occur during onset and progression of the overweight and obese states.
31661298	7	63	theme	kinase	1189:1194	arg1	action					1174:1179	the primary action	1162:1179	the primary action of this kinase to impact insulin sensitivity during weight gain	1162:1243	Our results using IKKεAlb-/- mice suggest that the primary action of this kinase to impact insulin sensitivity during weight gain lies predominantly within extrahepatic tissues.
31661298	0	64	from	content	93:99	arg1	tolerance					128:136	glucose tolerance	120:136	glucose tolerance	120:136	Hepatic IKKε expression is dispensable for high-fat feeding-induced increases in liver lipid content and alterations in glucose tolerance.
31661298	3	65	theme	first	606:610	arg1	line					618:621	the first mouse line	602:621	the first mouse line with conditional elimination of IKKε in the liver (IKKεAlb-/-)	602:684	To investigate the primary sites of action associated with IKKε during weight gain, we describe the first mouse line with conditional elimination of IKKε in the liver (IKKεAlb-/-).
31661298	1	66	theme	endocrine	149:157	arg1	changes					177:183	endocrine and immunological changes	149:183	endocrine and immunological changes that occur during onset and progression of the overweight and obese states	149:258	There are endocrine and immunological changes that occur during onset and progression of the overweight and obese states.
31661298	7	67	theme	impact	1199:1204	arg1	sensitivity					1214:1224	impact insulin sensitivity	1199:1224	impact insulin sensitivity	1199:1224	Our results using IKKεAlb-/- mice suggest that the primary action of this kinase to impact insulin sensitivity during weight gain lies predominantly within extrahepatic tissues.
31661298	1	68	theme	obese	247:251	arg1	states					253:258	the overweight and obese states	228:258	the overweight and obese states	228:258	There are endocrine and immunological changes that occur during onset and progression of the overweight and obese states.
31046899	8	0	theme	61.8 mol	1253:1260	arg1	genome					1264:1269	genome	1264:1269	genome	1264:1269	The DNA G+C content was 61.8 mol% (genome).
31046899	8	0	theme	61.8 mol	1253:1260	arg1	G+C content					1237:1247	The DNA G+C content	1229:1247	The DNA G+C content	1229:1247	The DNA G+C content was 61.8 mol% (genome).
31046899	8	0	theme	61.8 mol	1253:1260	arg1	%					1261:1261	61.8 mol%	1253:1261	61.8 mol% (genome)	1253:1270	The DNA G+C content was 61.8 mol% (genome).
31046899	3	1	dep	heterotrophic	340:352	arg1	heterotrophic					340:352	heterotrophic	340:352	heterotrophic	340:352	The isolate was heterotrophic, strictly aerobic, catalase-positive, oxidase-negative and formed orange-pigmented colonies in solid media.
31046899	3	1	dep	heterotrophic	340:352	arg1	aerobic					364:370	aerobic	364:370	aerobic	364:370	The isolate was heterotrophic, strictly aerobic, catalase-positive, oxidase-negative and formed orange-pigmented colonies in solid media.
31046899	3	1	dep	heterotrophic	340:352	arg1	isolate					328:334	The isolate	324:334	The isolate	324:334	The isolate was heterotrophic, strictly aerobic, catalase-positive, oxidase-negative and formed orange-pigmented colonies in solid media.
31046899	3	1	dep	heterotrophic	340:352	arg1	oxidase-negative					392:407	oxidase-negative	392:407	oxidase-negative	392:407	The isolate was heterotrophic, strictly aerobic, catalase-positive, oxidase-negative and formed orange-pigmented colonies in solid media.
31046899	12	2	theme	1.16706T=MCC	1648:1659	arg1	3367T					1661:1665	=JCM 32100T=CGMCC 1.16706T=MCC 3367T	1630:1665	=JCM 32100T=CGMCC 1.16706T=MCC 3367T	1630:1665	The type strain is M8T (=JCM 32100T=CGMCC 1.16706T=MCC 3367T).
31046899	12	2	theme	1.16706T=MCC	1648:1659	arg1	M8T					1625:1627	M8T	1625:1627	M8T (=JCM 32100T=CGMCC 1.16706T=MCC 3367T)	1625:1666	The type strain is M8T (=JCM 32100T=CGMCC 1.16706T=MCC 3367T).
31046899	10	3	dep	analyses	1454:1461	arg1	basis					1432:1436	basis	1432:1436	basis	1432:1436	On the basis of phylogenetic analyses and phenotypic data, strain M8T is considered to represent a novel species, for which the name Nesterenkonianatronophila sp.
31046899	10	3	dep	analyses	1454:1461	arg1	the					1428:1430	the	1428:1430	the	1428:1430	On the basis of phylogenetic analyses and phenotypic data, strain M8T is considered to represent a novel species, for which the name Nesterenkonianatronophila sp.
31046899	5	4	theme	w/v	608:610	arg1	NaCl					613:616	1-12 % (w/v) NaCl	600:616	1-12 % (w/v) NaCl	600:616	The organism grew at 10-38 °C, at pH 7.5-12.0 and in the presence of 1-12 % (w/v) NaCl, with optimal growth occurring at 30 °C, at pH 10 and in the presence of 5 % (w/v) NaCl.
31046899	6	5	theme	Comparative	707:717	arg1	analysis					742:749	Comparative 16S rRNA gene sequence analysis	707:749	Comparative 16S rRNA gene sequence analysis	707:749	Comparative 16S rRNA gene sequence analysis showed that strain M8T belonged to the genus Nesterenkonia, sharing the closest similarities to Nesterenkoniahalobia DSM 20541T, Nesterenkoniahalophila YIM 70179T and Nesterenkoniaaethiopica DSM 17733T (97.5, 97.5 and 97.1 %, respectively).
31046899	5	6	theme	NaCl	613:616	arg1	presence					588:595	the presence	584:595	the presence of 1-12 % (w/v) NaCl	584:616	The organism grew at 10-38 °C, at pH 7.5-12.0 and in the presence of 1-12 % (w/v) NaCl, with optimal growth occurring at 30 °C, at pH 10 and in the presence of 5 % (w/v) NaCl.
31046899	5	6	theme	NaCl	613:616	arg1	pH					565:566	pH 7.5-12.0	565:575	pH 7.5-12.0	565:575	The organism grew at 10-38 °C, at pH 7.5-12.0 and in the presence of 1-12 % (w/v) NaCl, with optimal growth occurring at 30 °C, at pH 10 and in the presence of 5 % (w/v) NaCl.
31046899	2	7	dep	Gram-stain-positive	150:168	arg1	alkaliphilic					171:182	alkaliphilic	171:182	alkaliphilic	171:182	A Gram-stain-positive, alkaliphilic, moderately halophilic, cocci-shaped actinobacterium (strain M8T) was isolated from a sample of soda lake sediment (Lake Magadi, Tanzania).
31046899	2	7	dep	Gram-stain-positive	150:168	arg1	halophilic					196:205	halophilic	196:205	halophilic	196:205	A Gram-stain-positive, alkaliphilic, moderately halophilic, cocci-shaped actinobacterium (strain M8T) was isolated from a sample of soda lake sediment (Lake Magadi, Tanzania).
31046899	2	7	dep	Gram-stain-positive	150:168	arg1	cocci-shaped					208:219	cocci-shaped	208:219	cocci-shaped	208:219	A Gram-stain-positive, alkaliphilic, moderately halophilic, cocci-shaped actinobacterium (strain M8T) was isolated from a sample of soda lake sediment (Lake Magadi, Tanzania).
31046899	10	8	theme	phylogenetic	1441:1452	arg1	analyses					1454:1461	phylogenetic analyses	1441:1461	phylogenetic analyses	1441:1461	On the basis of phylogenetic analyses and phenotypic data, strain M8T is considered to represent a novel species, for which the name Nesterenkonianatronophila sp.
31046899	6	9	theme	16S	719:721	arg1	analysis					742:749	Comparative 16S rRNA gene sequence analysis	707:749	Comparative 16S rRNA gene sequence analysis	707:749	Comparative 16S rRNA gene sequence analysis showed that strain M8T belonged to the genus Nesterenkonia, sharing the closest similarities to Nesterenkoniahalobia DSM 20541T, Nesterenkoniahalophila YIM 70179T and Nesterenkoniaaethiopica DSM 17733T (97.5, 97.5 and 97.1 %, respectively).
31046899	7	10	theme	diamino	1011:1017	arg1	acid					1019:1022	The characteristic diamino acid	992:1022	The characteristic diamino acid of strain M8T	992:1036	The characteristic diamino acid of strain M8T was found to be lysine and the polar lipids detected were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two unidentified glycolipids and two unidentified phospholipids.
31046899	7	10	theme	diamino	1011:1017	arg1	lysine					1054:1059	lysine	1054:1059	lysine	1054:1059	The characteristic diamino acid of strain M8T was found to be lysine and the polar lipids detected were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two unidentified glycolipids and two unidentified phospholipids.
31046899	2	11	theme	soda	280:283	arg1	Tanzania					313:320	Tanzania	313:320	Tanzania	313:320	A Gram-stain-positive, alkaliphilic, moderately halophilic, cocci-shaped actinobacterium (strain M8T) was isolated from a sample of soda lake sediment (Lake Magadi, Tanzania).
31046899	2	11	theme	soda	280:283	arg1	sediment					290:297	soda lake sediment	280:297	soda lake sediment (Lake Magadi, Tanzania)	280:321	A Gram-stain-positive, alkaliphilic, moderately halophilic, cocci-shaped actinobacterium (strain M8T) was isolated from a sample of soda lake sediment (Lake Magadi, Tanzania).
31046899	9	12	theme	respiratory	1322:1332	arg1	MK-10					1309:1313	MK-10	1309:1313	MK-10	1309:1313	The strain contained MK-7, MK-9 and MK-10 as the respiratory quinones, and the major fatty acids (>10 %) comprised anteiso-C17 : 0 and anteiso-C15 : 0.
31046899	9	12	theme	respiratory	1322:1332	arg1	MK-7					1294:1297	MK-7	1294:1297	MK-7	1294:1297	The strain contained MK-7, MK-9 and MK-10 as the respiratory quinones, and the major fatty acids (>10 %) comprised anteiso-C17 : 0 and anteiso-C15 : 0.
31046899	9	12	theme	respiratory	1322:1332	arg1	MK-9					1300:1303	MK-9	1300:1303	MK-9	1300:1303	The strain contained MK-7, MK-9 and MK-10 as the respiratory quinones, and the major fatty acids (>10 %) comprised anteiso-C17 : 0 and anteiso-C15 : 0.
31046899	9	12	theme	respiratory	1322:1332	arg1	quinones					1334:1341	the respiratory quinones	1318:1341	the respiratory quinones	1318:1341	The strain contained MK-7, MK-9 and MK-10 as the respiratory quinones, and the major fatty acids (>10 %) comprised anteiso-C17 : 0 and anteiso-C15 : 0.
31046899	10	13	theme	phenotypic	1467:1476	arg1	data					1478:1481	phenotypic data	1467:1481	phenotypic data	1467:1481	On the basis of phylogenetic analyses and phenotypic data, strain M8T is considered to represent a novel species, for which the name Nesterenkonianatronophila sp.
31046899	3	14	dep	aerobic	364:370	arg1	catalase-positive					373:389	catalase-positive	373:389	catalase-positive	373:389	The isolate was heterotrophic, strictly aerobic, catalase-positive, oxidase-negative and formed orange-pigmented colonies in solid media.
31046899	10	15	theme	strain	1484:1489	arg1	M8T					1491:1493	strain M8T	1484:1493	strain M8T	1484:1493	On the basis of phylogenetic analyses and phenotypic data, strain M8T is considered to represent a novel species, for which the name Nesterenkonianatronophila sp.
31046899	9	16	dep	anteiso-C17 	1388:1399	arg1	 0					1401:1402	 0	1401:1402	 0	1401:1402	The strain contained MK-7, MK-9 and MK-10 as the respiratory quinones, and the major fatty acids (>10 %) comprised anteiso-C17 : 0 and anteiso-C15 : 0.
31046899	9	16	dep	anteiso-C17 	1388:1399	arg1	 0					1421:1422	 0	1421:1422	anteiso-C17 : 0 and anteiso-C15 : 0	1388:1422	The strain contained MK-7, MK-9 and MK-10 as the respiratory quinones, and the major fatty acids (>10 %) comprised anteiso-C17 : 0 and anteiso-C15 : 0.
31046899	9	16	dep	anteiso-C17 	1388:1399	arg1	anteiso-C15 					1408:1419	anteiso-C15 	1408:1419	anteiso-C15 	1408:1419	The strain contained MK-7, MK-9 and MK-10 as the respiratory quinones, and the major fatty acids (>10 %) comprised anteiso-C17 : 0 and anteiso-C15 : 0.
31046899	9	17	theme	fatty	1358:1362	arg1	acids					1364:1368	the major fatty acids	1348:1368	the major fatty acids (>10 %)	1348:1376	The strain contained MK-7, MK-9 and MK-10 as the respiratory quinones, and the major fatty acids (>10 %) comprised anteiso-C17 : 0 and anteiso-C15 : 0.
31046899	9	17	theme	fatty	1358:1362	arg1	%					1375:1375	>10 %	1371:1375	>10 %	1371:1375	The strain contained MK-7, MK-9 and MK-10 as the respiratory quinones, and the major fatty acids (>10 %) comprised anteiso-C17 : 0 and anteiso-C15 : 0.
31046899	7	18	theme	polar	1069:1073	arg1	lipids					1075:1080	the polar lipids	1065:1080	the polar lipids detected	1065:1089	The characteristic diamino acid of strain M8T was found to be lysine and the polar lipids detected were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two unidentified glycolipids and two unidentified phospholipids.
31046899	7	18	theme	polar	1069:1073	arg1	diphosphatidylglycerol					1096:1117	diphosphatidylglycerol	1096:1117	diphosphatidylglycerol	1096:1117	The characteristic diamino acid of strain M8T was found to be lysine and the polar lipids detected were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two unidentified glycolipids and two unidentified phospholipids.
31046899	10	19	theme	Nesterenkonianatronophila	1558:1582	arg1	sp					1584:1585	the name Nesterenkonianatronophila sp	1549:1585	the name Nesterenkonianatronophila sp	1549:1585	On the basis of phylogenetic analyses and phenotypic data, strain M8T is considered to represent a novel species, for which the name Nesterenkonianatronophila sp.
31046899	3	20	theme	orange-pigmented	420:435	arg1	colonies					437:444	orange-pigmented colonies	420:444	orange-pigmented colonies	420:444	The isolate was heterotrophic, strictly aerobic, catalase-positive, oxidase-negative and formed orange-pigmented colonies in solid media.
31046899	5	21	theme	w/v	696:698	arg1	NaCl					701:704	5 % (w/v) NaCl	691:704	5 % (w/v) NaCl	691:704	The organism grew at 10-38 °C, at pH 7.5-12.0 and in the presence of 1-12 % (w/v) NaCl, with optimal growth occurring at 30 °C, at pH 10 and in the presence of 5 % (w/v) NaCl.
31046899	7	22	theme	characteristic	996:1009	arg1	acid					1019:1022	The characteristic diamino acid	992:1022	The characteristic diamino acid of strain M8T	992:1036	The characteristic diamino acid of strain M8T was found to be lysine and the polar lipids detected were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two unidentified glycolipids and two unidentified phospholipids.
31046899	7	22	theme	characteristic	996:1009	arg1	lysine					1054:1059	lysine	1054:1059	lysine	1054:1059	The characteristic diamino acid of strain M8T was found to be lysine and the polar lipids detected were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two unidentified glycolipids and two unidentified phospholipids.
31046899	5	23	theme	NaCl	701:704	arg1	presence					679:686	the presence	675:686	the presence of 5 % (w/v) NaCl	675:704	The organism grew at 10-38 °C, at pH 7.5-12.0 and in the presence of 1-12 % (w/v) NaCl, with optimal growth occurring at 30 °C, at pH 10 and in the presence of 5 % (w/v) NaCl.
31046899	0	24	theme	natronophila	14:25	arg1	sp					27:28	Nesterenkonia natronophila sp	0:28	Nesterenkonia natronophila sp.	0:29	Nesterenkonia natronophila sp.
31046899	4	25	theme	various	474:480	arg1	sources					522:528	sole carbon sources	510:528	sole carbon sources	510:528	It utilized various sugars and organic acids as sole carbon sources.
31046899	4	25	theme	various	474:480	arg1	sugars					482:487	various sugars	474:487	various sugars	474:487	It utilized various sugars and organic acids as sole carbon sources.
31046899	4	25	theme	various	474:480	arg1	acids					501:505	organic acids	493:505	organic acids	493:505	It utilized various sugars and organic acids as sole carbon sources.
31046899	5	26	theme	1-12 	600:604	arg1	NaCl					613:616	1-12 % (w/v) NaCl	600:616	1-12 % (w/v) NaCl	600:616	The organism grew at 10-38 °C, at pH 7.5-12.0 and in the presence of 1-12 % (w/v) NaCl, with optimal growth occurring at 30 °C, at pH 10 and in the presence of 5 % (w/v) NaCl.
31046899	1	27	theme	soda	85:88	arg1	lake					90:93	a soda lake	83:93	a soda lake	83:93	nov., an alkaliphilic actinobacterium isolated from a soda lake, and emended description of the genus Nesterenkonia.
31046899	0	28	theme	Nesterenkonia	0:12	arg1	sp					27:28	Nesterenkonia natronophila sp	0:28	Nesterenkonia natronophila sp.	0:29	Nesterenkonia natronophila sp.
31046899	1	29	attach	isolated	69:76	arg2	actinobacterium					53:67	an alkaliphilic actinobacterium	37:67	an alkaliphilic actinobacterium isolated from a soda lake	37:93	nov., an alkaliphilic actinobacterium isolated from a soda lake, and emended description of the genus Nesterenkonia.
31046899	1	29	attach	isolated	69:76	arg1	lake					90:93	a soda lake	83:93	a soda lake	83:93	nov., an alkaliphilic actinobacterium isolated from a soda lake, and emended description of the genus Nesterenkonia.
31046899	5	30	theme	%	605:605	arg1	NaCl					613:616	1-12 % (w/v) NaCl	600:616	1-12 % (w/v) NaCl	600:616	The organism grew at 10-38 °C, at pH 7.5-12.0 and in the presence of 1-12 % (w/v) NaCl, with optimal growth occurring at 30 °C, at pH 10 and in the presence of 5 % (w/v) NaCl.
31046899	12	31	theme	type	1610:1613	arg1	M8T					1625:1627	M8T	1625:1627	M8T (=JCM 32100T=CGMCC 1.16706T=MCC 3367T)	1625:1666	The type strain is M8T (=JCM 32100T=CGMCC 1.16706T=MCC 3367T).
31046899	12	31	theme	type	1610:1613	arg1	strain					1615:1620	The type strain	1606:1620	The type strain	1606:1620	The type strain is M8T (=JCM 32100T=CGMCC 1.16706T=MCC 3367T).
31046899	6	32	theme	97.1 	969:973	arg1	%					974:974	97.5, 97.5 and 97.1 %	954:974	%	974:974	Comparative 16S rRNA gene sequence analysis showed that strain M8T belonged to the genus Nesterenkonia, sharing the closest similarities to Nesterenkoniahalobia DSM 20541T, Nesterenkoniahalophila YIM 70179T and Nesterenkoniaaethiopica DSM 17733T (97.5, 97.5 and 97.1 %, respectively).
31046899	2	33	theme	sediment	290:297	arg1	sample					270:275	a sample	268:275	a sample of soda lake sediment (Lake Magadi, Tanzania)	268:321	A Gram-stain-positive, alkaliphilic, moderately halophilic, cocci-shaped actinobacterium (strain M8T) was isolated from a sample of soda lake sediment (Lake Magadi, Tanzania).
31046899	6	34	theme	genus	790:794	arg1	Nesterenkonia					796:808	the genus Nesterenkonia	786:808	the genus Nesterenkonia	786:808	Comparative 16S rRNA gene sequence analysis showed that strain M8T belonged to the genus Nesterenkonia, sharing the closest similarities to Nesterenkoniahalobia DSM 20541T, Nesterenkoniahalophila YIM 70179T and Nesterenkoniaaethiopica DSM 17733T (97.5, 97.5 and 97.1 %, respectively).
31046899	12	35	theme	=JCM	1630:1633	arg1	3367T					1661:1665	=JCM 32100T=CGMCC 1.16706T=MCC 3367T	1630:1665	=JCM 32100T=CGMCC 1.16706T=MCC 3367T	1630:1665	The type strain is M8T (=JCM 32100T=CGMCC 1.16706T=MCC 3367T).
31046899	12	35	theme	=JCM	1630:1633	arg1	M8T					1625:1627	M8T	1625:1627	M8T (=JCM 32100T=CGMCC 1.16706T=MCC 3367T)	1625:1666	The type strain is M8T (=JCM 32100T=CGMCC 1.16706T=MCC 3367T).
31046899	6	36	theme	DSM	868:870	arg1	20541T					872:877	Nesterenkoniahalobia DSM 20541T	847:877	Nesterenkoniahalobia DSM 20541T	847:877	Comparative 16S rRNA gene sequence analysis showed that strain M8T belonged to the genus Nesterenkonia, sharing the closest similarities to Nesterenkoniahalobia DSM 20541T, Nesterenkoniahalophila YIM 70179T and Nesterenkoniaaethiopica DSM 17733T (97.5, 97.5 and 97.1 %, respectively).
31046899	6	37	theme	strain	763:768	arg1	M8T					770:772	strain M8T	763:772	strain M8T	763:772	Comparative 16S rRNA gene sequence analysis showed that strain M8T belonged to the genus Nesterenkonia, sharing the closest similarities to Nesterenkoniahalobia DSM 20541T, Nesterenkoniahalophila YIM 70179T and Nesterenkoniaaethiopica DSM 17733T (97.5, 97.5 and 97.1 %, respectively).
31046899	2	38	theme	lake	285:288	arg1	Tanzania					313:320	Tanzania	313:320	Tanzania	313:320	A Gram-stain-positive, alkaliphilic, moderately halophilic, cocci-shaped actinobacterium (strain M8T) was isolated from a sample of soda lake sediment (Lake Magadi, Tanzania).
31046899	2	38	theme	lake	285:288	arg1	sediment					290:297	soda lake sediment	280:297	soda lake sediment (Lake Magadi, Tanzania)	280:321	A Gram-stain-positive, alkaliphilic, moderately halophilic, cocci-shaped actinobacterium (strain M8T) was isolated from a sample of soda lake sediment (Lake Magadi, Tanzania).
31046899	5	39	theme	5 	691:692	arg1	NaCl					701:704	5 % (w/v) NaCl	691:704	5 % (w/v) NaCl	691:704	The organism grew at 10-38 °C, at pH 7.5-12.0 and in the presence of 1-12 % (w/v) NaCl, with optimal growth occurring at 30 °C, at pH 10 and in the presence of 5 % (w/v) NaCl.
31046899	6	40	theme	Nesterenkoniahalobia	847:866	arg1	20541T					872:877	Nesterenkoniahalobia DSM 20541T	847:877	Nesterenkoniahalobia DSM 20541T	847:877	Comparative 16S rRNA gene sequence analysis showed that strain M8T belonged to the genus Nesterenkonia, sharing the closest similarities to Nesterenkoniahalobia DSM 20541T, Nesterenkoniahalophila YIM 70179T and Nesterenkoniaaethiopica DSM 17733T (97.5, 97.5 and 97.1 %, respectively).
31046899	9	41	theme	major	1352:1356	arg1	acids					1364:1368	the major fatty acids	1348:1368	the major fatty acids (>10 %)	1348:1376	The strain contained MK-7, MK-9 and MK-10 as the respiratory quinones, and the major fatty acids (>10 %) comprised anteiso-C17 : 0 and anteiso-C15 : 0.
31046899	9	41	theme	major	1352:1356	arg1	%					1375:1375	>10 %	1371:1375	>10 %	1371:1375	The strain contained MK-7, MK-9 and MK-10 as the respiratory quinones, and the major fatty acids (>10 %) comprised anteiso-C17 : 0 and anteiso-C15 : 0.
31046899	5	42	theme	%	693:693	arg1	NaCl					701:704	5 % (w/v) NaCl	691:704	5 % (w/v) NaCl	691:704	The organism grew at 10-38 °C, at pH 7.5-12.0 and in the presence of 1-12 % (w/v) NaCl, with optimal growth occurring at 30 °C, at pH 10 and in the presence of 5 % (w/v) NaCl.
31046899	4	43	theme	carbon	515:520	arg1	sources					522:528	sole carbon sources	510:528	sole carbon sources	510:528	It utilized various sugars and organic acids as sole carbon sources.
31046899	4	43	theme	carbon	515:520	arg1	sugars					482:487	various sugars	474:487	various sugars	474:487	It utilized various sugars and organic acids as sole carbon sources.
31046899	4	43	theme	carbon	515:520	arg1	acids					501:505	organic acids	493:505	organic acids	493:505	It utilized various sugars and organic acids as sole carbon sources.
31046899	2	44	theme	strain	238:243	arg1	actinobacterium					221:235	A Gram-stain-positive, alkaliphilic, moderately halophilic, cocci-shaped actinobacterium	148:235	A Gram-stain-positive, alkaliphilic, moderately halophilic, cocci-shaped actinobacterium (strain M8T)	148:248	A Gram-stain-positive, alkaliphilic, moderately halophilic, cocci-shaped actinobacterium (strain M8T) was isolated from a sample of soda lake sediment (Lake Magadi, Tanzania).
31046899	2	44	theme	strain	238:243	arg1	M8T					245:247	strain M8T	238:247	strain M8T	238:247	A Gram-stain-positive, alkaliphilic, moderately halophilic, cocci-shaped actinobacterium (strain M8T) was isolated from a sample of soda lake sediment (Lake Magadi, Tanzania).
31046899	3	45	theme	solid	449:453	arg1	media					455:459	solid media	449:459	solid media	449:459	The isolate was heterotrophic, strictly aerobic, catalase-positive, oxidase-negative and formed orange-pigmented colonies in solid media.
31046899	7	46	theme	strain	1027:1032	arg1	M8T					1034:1036	strain M8T	1027:1036	strain M8T	1027:1036	The characteristic diamino acid of strain M8T was found to be lysine and the polar lipids detected were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two unidentified glycolipids and two unidentified phospholipids.
31046899	4	47	theme	sole	510:513	arg1	sources					522:528	sole carbon sources	510:528	sole carbon sources	510:528	It utilized various sugars and organic acids as sole carbon sources.
31046899	4	47	theme	sole	510:513	arg1	sugars					482:487	various sugars	474:487	various sugars	474:487	It utilized various sugars and organic acids as sole carbon sources.
31046899	4	47	theme	sole	510:513	arg1	acids					501:505	organic acids	493:505	organic acids	493:505	It utilized various sugars and organic acids as sole carbon sources.
31046899	2	48	theme	Gram-stain-positive	150:168	arg1	actinobacterium					221:235	A Gram-stain-positive, alkaliphilic, moderately halophilic, cocci-shaped actinobacterium	148:235	A Gram-stain-positive, alkaliphilic, moderately halophilic, cocci-shaped actinobacterium (strain M8T)	148:248	A Gram-stain-positive, alkaliphilic, moderately halophilic, cocci-shaped actinobacterium (strain M8T) was isolated from a sample of soda lake sediment (Lake Magadi, Tanzania).
31046899	2	48	theme	Gram-stain-positive	150:168	arg1	M8T					245:247	strain M8T	238:247	strain M8T	238:247	A Gram-stain-positive, alkaliphilic, moderately halophilic, cocci-shaped actinobacterium (strain M8T) was isolated from a sample of soda lake sediment (Lake Magadi, Tanzania).
31046899	1	49	theme	emended	100:106	arg1	description					108:118	emended description	100:118	emended description of the genus Nesterenkonia	100:145	nov., an alkaliphilic actinobacterium isolated from a soda lake, and emended description of the genus Nesterenkonia.
31046899	4	50	used	utilized	465:472	arg2	It					462:463	It	462:463	It	462:463	It utilized various sugars and organic acids as sole carbon sources.
31046899	6	51	theme	closest	823:829	arg1	similarities					831:842	the closest similarities	819:842	the closest similarities to Nesterenkoniahalobia DSM 20541T, Nesterenkoniahalophila YIM 70179T and Nesterenkoniaaethiopica DSM 17733T (97.5, 97.5 and 97.1 %, respectively)	819:989	Comparative 16S rRNA gene sequence analysis showed that strain M8T belonged to the genus Nesterenkonia, sharing the closest similarities to Nesterenkoniahalobia DSM 20541T, Nesterenkoniahalophila YIM 70179T and Nesterenkoniaaethiopica DSM 17733T (97.5, 97.5 and 97.1 %, respectively).
31046899	6	52	theme	sequence	733:740	arg1	analysis					742:749	Comparative 16S rRNA gene sequence analysis	707:749	Comparative 16S rRNA gene sequence analysis	707:749	Comparative 16S rRNA gene sequence analysis showed that strain M8T belonged to the genus Nesterenkonia, sharing the closest similarities to Nesterenkoniahalobia DSM 20541T, Nesterenkoniahalophila YIM 70179T and Nesterenkoniaaethiopica DSM 17733T (97.5, 97.5 and 97.1 %, respectively).
31046899	6	53	dep	20541T	872:877	arg1	%					974:974	97.5, 97.5 and 97.1 %	954:974	%	974:974	Comparative 16S rRNA gene sequence analysis showed that strain M8T belonged to the genus Nesterenkonia, sharing the closest similarities to Nesterenkoniahalobia DSM 20541T, Nesterenkoniahalophila YIM 70179T and Nesterenkoniaaethiopica DSM 17733T (97.5, 97.5 and 97.1 %, respectively).
31046899	7	54	theme	unidentified	1201:1212	arg1	phospholipids					1214:1226	two unidentified phospholipids	1197:1226	two unidentified phospholipids	1197:1226	The characteristic diamino acid of strain M8T was found to be lysine and the polar lipids detected were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two unidentified glycolipids and two unidentified phospholipids.
31046899	7	54	theme	unidentified	1201:1212	arg1	diphosphatidylglycerol					1096:1117	diphosphatidylglycerol	1096:1117	diphosphatidylglycerol	1096:1117	The characteristic diamino acid of strain M8T was found to be lysine and the polar lipids detected were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two unidentified glycolipids and two unidentified phospholipids.
31046899	5	55	theme	optimal	624:630	arg1	growth					632:637	optimal growth	624:637	optimal growth occurring at 30 °C	624:656	The organism grew at 10-38 °C, at pH 7.5-12.0 and in the presence of 1-12 % (w/v) NaCl, with optimal growth occurring at 30 °C, at pH 10 and in the presence of 5 % (w/v) NaCl.
31046899	6	56	theme	gene	728:731	arg1	analysis					742:749	Comparative 16S rRNA gene sequence analysis	707:749	Comparative 16S rRNA gene sequence analysis	707:749	Comparative 16S rRNA gene sequence analysis showed that strain M8T belonged to the genus Nesterenkonia, sharing the closest similarities to Nesterenkoniahalobia DSM 20541T, Nesterenkoniahalophila YIM 70179T and Nesterenkoniaaethiopica DSM 17733T (97.5, 97.5 and 97.1 %, respectively).
31046899	8	57	theme	DNA	1233:1235	arg1	G+C content					1237:1247	The DNA G+C content	1229:1247	The DNA G+C content	1229:1247	The DNA G+C content was 61.8 mol% (genome).
31046899	8	57	theme	DNA	1233:1235	arg1	%					1261:1261	61.8 mol%	1253:1261	61.8 mol% (genome)	1253:1270	The DNA G+C content was 61.8 mol% (genome).
31046899	2	58	attach	isolated	254:261	arg2	actinobacterium					221:235	A Gram-stain-positive, alkaliphilic, moderately halophilic, cocci-shaped actinobacterium	148:235	A Gram-stain-positive, alkaliphilic, moderately halophilic, cocci-shaped actinobacterium (strain M8T)	148:248	A Gram-stain-positive, alkaliphilic, moderately halophilic, cocci-shaped actinobacterium (strain M8T) was isolated from a sample of soda lake sediment (Lake Magadi, Tanzania).
31046899	2	58	attach	isolated	254:261	arg2	M8T					245:247	strain M8T	238:247	strain M8T	238:247	A Gram-stain-positive, alkaliphilic, moderately halophilic, cocci-shaped actinobacterium (strain M8T) was isolated from a sample of soda lake sediment (Lake Magadi, Tanzania).
31046899	2	58	attach	isolated	254:261	arg1	sample					270:275	a sample	268:275	a sample of soda lake sediment (Lake Magadi, Tanzania)	268:321	A Gram-stain-positive, alkaliphilic, moderately halophilic, cocci-shaped actinobacterium (strain M8T) was isolated from a sample of soda lake sediment (Lake Magadi, Tanzania).
31046899	6	59	theme	rRNA	723:726	arg1	analysis					742:749	Comparative 16S rRNA gene sequence analysis	707:749	Comparative 16S rRNA gene sequence analysis	707:749	Comparative 16S rRNA gene sequence analysis showed that strain M8T belonged to the genus Nesterenkonia, sharing the closest similarities to Nesterenkoniahalobia DSM 20541T, Nesterenkoniahalophila YIM 70179T and Nesterenkoniaaethiopica DSM 17733T (97.5, 97.5 and 97.1 %, respectively).
31046899	10	60	theme	name	1553:1556	arg1	sp					1584:1585	the name Nesterenkonianatronophila sp	1549:1585	the name Nesterenkonianatronophila sp	1549:1585	On the basis of phylogenetic analyses and phenotypic data, strain M8T is considered to represent a novel species, for which the name Nesterenkonianatronophila sp.
31046899	10	61	theme	novel	1524:1528	arg1	species					1530:1536	a novel species	1522:1536	a novel species	1522:1536	On the basis of phylogenetic analyses and phenotypic data, strain M8T is considered to represent a novel species, for which the name Nesterenkonianatronophila sp.
31046899	7	62	theme	unidentified	1168:1179	arg1	diphosphatidylglycerol					1096:1117	diphosphatidylglycerol	1096:1117	diphosphatidylglycerol	1096:1117	The characteristic diamino acid of strain M8T was found to be lysine and the polar lipids detected were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two unidentified glycolipids and two unidentified phospholipids.
31046899	7	62	theme	unidentified	1168:1179	arg1	glycolipids					1181:1191	two unidentified glycolipids	1164:1191	two unidentified glycolipids	1164:1191	The characteristic diamino acid of strain M8T was found to be lysine and the polar lipids detected were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two unidentified glycolipids and two unidentified phospholipids.
31046899	12	63	theme	32100T=CGMCC	1635:1646	arg1	3367T					1661:1665	=JCM 32100T=CGMCC 1.16706T=MCC 3367T	1630:1665	=JCM 32100T=CGMCC 1.16706T=MCC 3367T	1630:1665	The type strain is M8T (=JCM 32100T=CGMCC 1.16706T=MCC 3367T).
31046899	12	63	theme	32100T=CGMCC	1635:1646	arg1	M8T					1625:1627	M8T	1625:1627	M8T (=JCM 32100T=CGMCC 1.16706T=MCC 3367T)	1625:1666	The type strain is M8T (=JCM 32100T=CGMCC 1.16706T=MCC 3367T).
31046899	6	64	theme	97.5	954:957	arg1	%					974:974	97.5, 97.5 and 97.1 %	954:974	%	974:974	Comparative 16S rRNA gene sequence analysis showed that strain M8T belonged to the genus Nesterenkonia, sharing the closest similarities to Nesterenkoniahalobia DSM 20541T, Nesterenkoniahalophila YIM 70179T and Nesterenkoniaaethiopica DSM 17733T (97.5, 97.5 and 97.1 %, respectively).
31046899	9	65	contain	contained	1284:1292	arg2	MK-7					1294:1297	MK-7	1294:1297	MK-7	1294:1297	The strain contained MK-7, MK-9 and MK-10 as the respiratory quinones, and the major fatty acids (>10 %) comprised anteiso-C17 : 0 and anteiso-C15 : 0.
31046899	9	65	contain	contained	1284:1292	arg1	strain					1277:1282	The strain	1273:1282	The strain	1273:1282	The strain contained MK-7, MK-9 and MK-10 as the respiratory quinones, and the major fatty acids (>10 %) comprised anteiso-C17 : 0 and anteiso-C15 : 0.
31046899	9	65	contain	contained	1284:1292	arg2	quinones					1334:1341	the respiratory quinones	1318:1341	the respiratory quinones	1318:1341	The strain contained MK-7, MK-9 and MK-10 as the respiratory quinones, and the major fatty acids (>10 %) comprised anteiso-C17 : 0 and anteiso-C15 : 0.
31046899	9	65	contain	contained	1284:1292	arg2	MK-10					1309:1313	MK-10	1309:1313	MK-10	1309:1313	The strain contained MK-7, MK-9 and MK-10 as the respiratory quinones, and the major fatty acids (>10 %) comprised anteiso-C17 : 0 and anteiso-C15 : 0.
31046899	9	65	contain	contained	1284:1292	arg2	MK-9					1300:1303	MK-9	1300:1303	MK-9	1300:1303	The strain contained MK-7, MK-9 and MK-10 as the respiratory quinones, and the major fatty acids (>10 %) comprised anteiso-C17 : 0 and anteiso-C15 : 0.
31046899	1	66	theme	genus	127:131	arg1	Nesterenkonia					133:145	the genus Nesterenkonia	123:145	the genus Nesterenkonia	123:145	nov., an alkaliphilic actinobacterium isolated from a soda lake, and emended description of the genus Nesterenkonia.
31046899	6	67	theme	DSM	942:944	arg1	17733T					946:951	Nesterenkoniaaethiopica DSM 17733T	918:951	Nesterenkoniaaethiopica DSM 17733T	918:951	Comparative 16S rRNA gene sequence analysis showed that strain M8T belonged to the genus Nesterenkonia, sharing the closest similarities to Nesterenkoniahalobia DSM 20541T, Nesterenkoniahalophila YIM 70179T and Nesterenkoniaaethiopica DSM 17733T (97.5, 97.5 and 97.1 %, respectively).
31046899	7	68	theme	M8T	1034:1036	arg1	acid					1019:1022	The characteristic diamino acid	992:1022	The characteristic diamino acid of strain M8T	992:1036	The characteristic diamino acid of strain M8T was found to be lysine and the polar lipids detected were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two unidentified glycolipids and two unidentified phospholipids.
31046899	7	68	theme	M8T	1034:1036	arg1	lysine					1054:1059	lysine	1054:1059	lysine	1054:1059	The characteristic diamino acid of strain M8T was found to be lysine and the polar lipids detected were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two unidentified glycolipids and two unidentified phospholipids.
31046899	4	69	theme	organic	493:499	arg1	sources					522:528	sole carbon sources	510:528	sole carbon sources	510:528	It utilized various sugars and organic acids as sole carbon sources.
31046899	4	69	theme	organic	493:499	arg1	sugars					482:487	various sugars	474:487	various sugars	474:487	It utilized various sugars and organic acids as sole carbon sources.
31046899	4	69	theme	organic	493:499	arg1	acids					501:505	organic acids	493:505	organic acids	493:505	It utilized various sugars and organic acids as sole carbon sources.
31046899	1	70	theme	alkaliphilic	40:51	arg1	actinobacterium					53:67	an alkaliphilic actinobacterium	37:67	an alkaliphilic actinobacterium isolated from a soda lake	37:93	nov., an alkaliphilic actinobacterium isolated from a soda lake, and emended description of the genus Nesterenkonia.
31046899	1	71	theme	Nesterenkonia	133:145	arg1	actinobacterium					53:67	an alkaliphilic actinobacterium	37:67	an alkaliphilic actinobacterium isolated from a soda lake	37:93	nov., an alkaliphilic actinobacterium isolated from a soda lake, and emended description of the genus Nesterenkonia.
31046899	1	71	theme	Nesterenkonia	133:145	arg1	description					108:118	emended description	100:118	emended description of the genus Nesterenkonia	100:145	nov., an alkaliphilic actinobacterium isolated from a soda lake, and emended description of the genus Nesterenkonia.
31046899	1	71	theme	Nesterenkonia	133:145	arg1	nov.					31:34	nov.	31:34	nov.	31:34	nov., an alkaliphilic actinobacterium isolated from a soda lake, and emended description of the genus Nesterenkonia.
31046899	6	72	theme	Nesterenkoniaaethiopica	918:940	arg1	17733T					946:951	Nesterenkoniaaethiopica DSM 17733T	918:951	Nesterenkoniaaethiopica DSM 17733T	918:951	Comparative 16S rRNA gene sequence analysis showed that strain M8T belonged to the genus Nesterenkonia, sharing the closest similarities to Nesterenkoniahalobia DSM 20541T, Nesterenkoniahalophila YIM 70179T and Nesterenkoniaaethiopica DSM 17733T (97.5, 97.5 and 97.1 %, respectively).
30785391	10	0	theme	Litorihabitans	1459:1472	arg1	nov.					1491:1494	Litorihabitans aurantiacus gen. nov.	1459:1494	the name Litorihabitans aurantiacus gen. nov.	1450:1494	Therefore, strain HIsM16-52T is concluded to represent a novel genus and species of the family Beutenbergiaceae, for which the name Litorihabitans aurantiacus gen. nov., sp.
30785391	4	1	theme	major	475:479	arg1	anteiso-C15 					498:509	anteiso-C15 	498:509	anteiso-C15 	498:509	The predominant isoprenoid quinone was MK-8(H4) and the major fatty acids were anteiso-C15 : 0, C16 : 0 and C14 : 0.
30785391	4	1	theme	major	475:479	arg1	acids					487:491	the major fatty acids	471:491	the major fatty acids	471:491	The predominant isoprenoid quinone was MK-8(H4) and the major fatty acids were anteiso-C15 : 0, C16 : 0 and C14 : 0.
30785391	3	2	theme	Strain	311:316	arg1	HIsM16-52T					318:327	Strain HIsM16-52T	311:327	Strain HIsM16-52T	311:327	Strain HIsM16-52T contained both lysine and ornithine as the diagnostic diamino acids of the peptidoglycan.
30785391	8	3	theme	16S	960:962	arg1	rRNA					964:967	The highest 16S rRNA	948:967	The highest 16S rRNA gene sequence similarities	948:994	The highest 16S rRNA gene sequence similarities were obtained to species of the genus Serinibacter(97.8-97.9 %), followed by the genera Miniimonas (97.0 %), Beutenbergia (96.4 %) and Salana (95.9 %).
30785391	7	4	theme	family	850:855	arg1	Beutenbergiaceae					857:872	the family Beutenbergiaceae	846:872	the family Beutenbergiaceae	846:872	Phylogenetic analyses based on 16S rRNA gene sequence comparison revealed that strain HIsM16-52T fell within the cluster of the family Beutenbergiaceae and formed a reliable cluster with the members of the genus Serinibacter.
30785391	4	5	dep	anteiso-C15 	498:509	arg1	 0					511:512	 0	511:512	 0	511:512	The predominant isoprenoid quinone was MK-8(H4) and the major fatty acids were anteiso-C15 : 0, C16 : 0 and C14 : 0.
30785391	4	5	dep	anteiso-C15 	498:509	arg1	C14 					527:530	C14 	527:530	C14 	527:530	The predominant isoprenoid quinone was MK-8(H4) and the major fatty acids were anteiso-C15 : 0, C16 : 0 and C14 : 0.
30785391	4	5	dep	anteiso-C15 	498:509	arg1	C16 					515:518	C16 	515:518	C16 	515:518	The predominant isoprenoid quinone was MK-8(H4) and the major fatty acids were anteiso-C15 : 0, C16 : 0 and C14 : 0.
30785391	9	6	from	Beutenbergiaceae	1266:1281	arg1	terms					1286:1290	terms	1286:1290	terms of chemotaxonomic characteristics	1286:1324	However, strain HIsM16-52T differed from the members of the genus Serinibacter and the other genera within the family Beutenbergiaceae in terms of chemotaxonomic characteristics.
30785391	3	7	theme	diamino	383:389	arg1	lysine					344:349	lysine	344:349	lysine	344:349	Strain HIsM16-52T contained both lysine and ornithine as the diagnostic diamino acids of the peptidoglycan.
30785391	3	7	theme	diamino	383:389	arg1	acids					391:395	the diagnostic diamino acids	368:395	the diagnostic diamino acids of the peptidoglycan	368:416	Strain HIsM16-52T contained both lysine and ornithine as the diagnostic diamino acids of the peptidoglycan.
30785391	3	7	theme	diamino	383:389	arg1	ornithine					355:363	ornithine	355:363	ornithine	355:363	Strain HIsM16-52T contained both lysine and ornithine as the diagnostic diamino acids of the peptidoglycan.
30785391	7	8	theme	Beutenbergiaceae	857:872	arg1	cluster					835:841	the cluster	831:841	the cluster of the family Beutenbergiaceae	831:872	Phylogenetic analyses based on 16S rRNA gene sequence comparison revealed that strain HIsM16-52T fell within the cluster of the family Beutenbergiaceae and formed a reliable cluster with the members of the genus Serinibacter.
30785391	9	9	theme	Serinibacter	1214:1225	arg1	members					1193:1199	the members	1189:1199	the members of the genus Serinibacter	1189:1225	However, strain HIsM16-52T differed from the members of the genus Serinibacter and the other genera within the family Beutenbergiaceae in terms of chemotaxonomic characteristics.
30785391	9	9	theme	Serinibacter	1214:1225	arg1	genera					1241:1246	the other genera	1231:1246	the other genera within the family Beutenbergiaceae in terms of chemotaxonomic characteristics	1231:1324	However, strain HIsM16-52T differed from the members of the genus Serinibacter and the other genera within the family Beutenbergiaceae in terms of chemotaxonomic characteristics.
30785391	5	10	theme	polar	549:553	arg1	phosphatidylglycerol					567:586	phosphatidylglycerol	567:586	phosphatidylglycerol	567:586	The detected polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol and two unidentified phospholipids.
30785391	5	10	theme	polar	549:553	arg1	lipids					555:560	The detected polar lipids	536:560	The detected polar lipids	536:560	The detected polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol and two unidentified phospholipids.
30785391	8	11	dep	obtained	1001:1008	arg1	followed					1061:1068	followed	1061:1068	followed by the genera Miniimonas (97.0 %), Beutenbergia (96.4 %) and Salana (95.9 %)	1061:1145	The highest 16S rRNA gene sequence similarities were obtained to species of the genus Serinibacter(97.8-97.9 %), followed by the genera Miniimonas (97.0 %), Beutenbergia (96.4 %) and Salana (95.9 %).
30785391	12	12	theme	112290T=TBRC	1574:1585	arg1	HIsM16-52T					1556:1565	HIsM16-52T	1556:1565	HIsM16-52T (=NBRC 112290T=TBRC 7759T)	1556:1592	The type strain of L. aurantiacus is HIsM16-52T (=NBRC 112290T=TBRC 7759T).
30785391	12	12	theme	112290T=TBRC	1574:1585	arg1	7759T					1587:1591	=NBRC 112290T=TBRC 7759T	1568:1591	=NBRC 112290T=TBRC 7759T	1568:1591	The type strain of L. aurantiacus is HIsM16-52T (=NBRC 112290T=TBRC 7759T).
30785391	12	13	theme	=NBRC	1568:1572	arg1	HIsM16-52T					1556:1565	HIsM16-52T	1556:1565	HIsM16-52T (=NBRC 112290T=TBRC 7759T)	1556:1592	The type strain of L. aurantiacus is HIsM16-52T (=NBRC 112290T=TBRC 7759T).
30785391	12	13	theme	=NBRC	1568:1572	arg1	7759T					1587:1591	=NBRC 112290T=TBRC 7759T	1568:1591	=NBRC 112290T=TBRC 7759T	1568:1591	The type strain of L. aurantiacus is HIsM16-52T (=NBRC 112290T=TBRC 7759T).
30785391	8	14	theme	Serinibacter	1034:1045	arg1	species					1013:1019	species	1013:1019	species of the genus Serinibacter(97.8-97.9 %)	1013:1058	The highest 16S rRNA gene sequence similarities were obtained to species of the genus Serinibacter(97.8-97.9 %), followed by the genera Miniimonas (97.0 %), Beutenbergia (96.4 %) and Salana (95.9 %).
30785391	7	15	theme	Serinibacter	934:945	arg1	members					913:919	the members	909:919	the members of the genus Serinibacter	909:945	Phylogenetic analyses based on 16S rRNA gene sequence comparison revealed that strain HIsM16-52T fell within the cluster of the family Beutenbergiaceae and formed a reliable cluster with the members of the genus Serinibacter.
30785391	2	16	theme	novel	126:130	arg1	actinobacterium					132:146	A novel actinobacterium	124:146	A novel actinobacterium	124:146	A novel actinobacterium, designated HIsM16-52T, was isolated from beach sand collected from Ishigaki Island in Japan and its taxonomic position was investigated by a polyphasic approach.
30785391	8	17	theme	genus	1028:1032	arg1	Serinibacter					1034:1045	the genus Serinibacter	1024:1045	the genus Serinibacter(97.8-97.9 %)	1024:1058	The highest 16S rRNA gene sequence similarities were obtained to species of the genus Serinibacter(97.8-97.9 %), followed by the genera Miniimonas (97.0 %), Beutenbergia (96.4 %) and Salana (95.9 %).
30785391	8	17	theme	genus	1028:1032	arg1	%					1057:1057	97.8-97.9 %	1047:1057	97.8-97.9 %	1047:1057	The highest 16S rRNA gene sequence similarities were obtained to species of the genus Serinibacter(97.8-97.9 %), followed by the genera Miniimonas (97.0 %), Beutenbergia (96.4 %) and Salana (95.9 %).
30785391	2	18	theme	taxonomic	249:257	arg1	position					259:266	its taxonomic position	245:266	its taxonomic position	245:266	A novel actinobacterium, designated HIsM16-52T, was isolated from beach sand collected from Ishigaki Island in Japan and its taxonomic position was investigated by a polyphasic approach.
30785391	2	19	from	Island	225:230	arg1	Japan					235:239	Japan	235:239	Japan	235:239	A novel actinobacterium, designated HIsM16-52T, was isolated from beach sand collected from Ishigaki Island in Japan and its taxonomic position was investigated by a polyphasic approach.
30785391	0	20	theme	aurantiacus	15:25	arg1	sp					38:39	sp	38:39	sp	38:39	Litorihabitans aurantiacus gen. nov., sp.
30785391	0	20	theme	aurantiacus	15:25	arg1	nov.					32:35	Litorihabitans aurantiacus gen. nov.	0:35	Litorihabitans aurantiacus gen. nov.	0:35	Litorihabitans aurantiacus gen. nov., sp.
30785391	9	21	theme	other	1235:1239	arg1	genera					1241:1246	the other genera	1231:1246	the other genera within the family Beutenbergiaceae in terms of chemotaxonomic characteristics	1231:1324	However, strain HIsM16-52T differed from the members of the genus Serinibacter and the other genera within the family Beutenbergiaceae in terms of chemotaxonomic characteristics.
30785391	8	22	theme	sequence	974:981	arg1	similarities					983:994	The highest 16S rRNA gene sequence similarities	948:994	The highest 16S rRNA gene sequence similarities	948:994	The highest 16S rRNA gene sequence similarities were obtained to species of the genus Serinibacter(97.8-97.9 %), followed by the genera Miniimonas (97.0 %), Beutenbergia (96.4 %) and Salana (95.9 %).
30785391	6	23	theme	DNA	674:676	arg1	G+C content					678:688	The DNA G+C content	670:688	The DNA G+C content	670:688	The DNA G+C content was determined to be 73.4 mol%.
30785391	6	23	theme	DNA	674:676	arg1	%					719:719	73.4 mol%	711:719	73.4 mol%	711:719	The DNA G+C content was determined to be 73.4 mol%.
30785391	2	24	attach	isolated	176:183	arg2	actinobacterium					132:146	A novel actinobacterium	124:146	A novel actinobacterium	124:146	A novel actinobacterium, designated HIsM16-52T, was isolated from beach sand collected from Ishigaki Island in Japan and its taxonomic position was investigated by a polyphasic approach.
30785391	2	24	attach	isolated	176:183	arg1	sand					196:199	beach sand	190:199	beach sand collected from Ishigaki Island in Japan	190:239	A novel actinobacterium, designated HIsM16-52T, was isolated from beach sand collected from Ishigaki Island in Japan and its taxonomic position was investigated by a polyphasic approach.
30785391	0	25	theme	Litorihabitans	0:13	arg1	sp					38:39	sp	38:39	sp	38:39	Litorihabitans aurantiacus gen. nov., sp.
30785391	0	25	theme	Litorihabitans	0:13	arg1	nov.					32:35	Litorihabitans aurantiacus gen. nov.	0:35	Litorihabitans aurantiacus gen. nov.	0:35	Litorihabitans aurantiacus gen. nov., sp.
30785391	12	26	theme	type	1523:1526	arg1	HIsM16-52T					1556:1565	HIsM16-52T	1556:1565	HIsM16-52T (=NBRC 112290T=TBRC 7759T)	1556:1592	The type strain of L. aurantiacus is HIsM16-52T (=NBRC 112290T=TBRC 7759T).
30785391	12	26	theme	type	1523:1526	arg1	strain					1528:1533	The type strain	1519:1533	The type strain of L. aurantiacus	1519:1551	The type strain of L. aurantiacus is HIsM16-52T (=NBRC 112290T=TBRC 7759T).
30785391	10	27	theme	novel	1384:1388	arg1	genus					1390:1394	a novel genus	1382:1394	a novel genus	1382:1394	Therefore, strain HIsM16-52T is concluded to represent a novel genus and species of the family Beutenbergiaceae, for which the name Litorihabitans aurantiacus gen. nov., sp.
30785391	8	28	theme	highest	952:958	arg1	rRNA					964:967	The highest 16S rRNA	948:967	The highest 16S rRNA gene sequence similarities	948:994	The highest 16S rRNA gene sequence similarities were obtained to species of the genus Serinibacter(97.8-97.9 %), followed by the genera Miniimonas (97.0 %), Beutenbergia (96.4 %) and Salana (95.9 %).
30785391	2	29	theme	polyphasic	290:299	arg1	approach					301:308	a polyphasic approach	288:308	a polyphasic approach	288:308	A novel actinobacterium, designated HIsM16-52T, was isolated from beach sand collected from Ishigaki Island in Japan and its taxonomic position was investigated by a polyphasic approach.
30785391	12	30	theme	aurantiacus	1541:1551	arg1	HIsM16-52T					1556:1565	HIsM16-52T	1556:1565	HIsM16-52T (=NBRC 112290T=TBRC 7759T)	1556:1592	The type strain of L. aurantiacus is HIsM16-52T (=NBRC 112290T=TBRC 7759T).
30785391	12	30	theme	aurantiacus	1541:1551	arg1	strain					1528:1533	The type strain	1519:1533	The type strain of L. aurantiacus	1519:1551	The type strain of L. aurantiacus is HIsM16-52T (=NBRC 112290T=TBRC 7759T).
30785391	7	31	theme	genus	928:932	arg1	Serinibacter					934:945	the genus Serinibacter	924:945	the genus Serinibacter	924:945	Phylogenetic analyses based on 16S rRNA gene sequence comparison revealed that strain HIsM16-52T fell within the cluster of the family Beutenbergiaceae and formed a reliable cluster with the members of the genus Serinibacter.
30785391	10	32	theme	Beutenbergiaceae	1422:1437	arg1	genus					1390:1394	a novel genus	1382:1394	a novel genus	1382:1394	Therefore, strain HIsM16-52T is concluded to represent a novel genus and species of the family Beutenbergiaceae, for which the name Litorihabitans aurantiacus gen. nov., sp.
30785391	10	32	theme	Beutenbergiaceae	1422:1437	arg1	species					1400:1406	species	1400:1406	species	1400:1406	Therefore, strain HIsM16-52T is concluded to represent a novel genus and species of the family Beutenbergiaceae, for which the name Litorihabitans aurantiacus gen. nov., sp.
30785391	8	33	theme	rRNA	964:967	arg1	similarities					983:994	The highest 16S rRNA gene sequence similarities	948:994	The highest 16S rRNA gene sequence similarities	948:994	The highest 16S rRNA gene sequence similarities were obtained to species of the genus Serinibacter(97.8-97.9 %), followed by the genera Miniimonas (97.0 %), Beutenbergia (96.4 %) and Salana (95.9 %).
30785391	1	34	theme	Beutenbergiaceae	81:96	arg1	actinobacterium					51:65	an actinobacterium	48:65	an actinobacterium of the family Beutenbergiaceae isolated from beach sand	48:121	nov., an actinobacterium of the family Beutenbergiaceae isolated from beach sand.
30785391	1	34	theme	Beutenbergiaceae	81:96	arg1	nov.					42:45	nov.	42:45	nov.	42:45	nov., an actinobacterium of the family Beutenbergiaceae isolated from beach sand.
30785391	1	35	theme	family	74:79	arg1	Beutenbergiaceae					81:96	the family Beutenbergiaceae	70:96	the family Beutenbergiaceae isolated from beach sand	70:121	nov., an actinobacterium of the family Beutenbergiaceae isolated from beach sand.
30785391	7	36	theme	sequence	767:774	arg1	comparison					776:785	16S rRNA gene sequence comparison	753:785	16S rRNA gene sequence comparison	753:785	Phylogenetic analyses based on 16S rRNA gene sequence comparison revealed that strain HIsM16-52T fell within the cluster of the family Beutenbergiaceae and formed a reliable cluster with the members of the genus Serinibacter.
30785391	10	37	theme	family	1415:1420	arg1	Beutenbergiaceae					1422:1437	the family Beutenbergiaceae	1411:1437	the family Beutenbergiaceae	1411:1437	Therefore, strain HIsM16-52T is concluded to represent a novel genus and species of the family Beutenbergiaceae, for which the name Litorihabitans aurantiacus gen. nov., sp.
30785391	7	38	theme	strain	801:806	arg1	HIsM16-52T					808:817	strain HIsM16-52T	801:817	strain HIsM16-52T	801:817	Phylogenetic analyses based on 16S rRNA gene sequence comparison revealed that strain HIsM16-52T fell within the cluster of the family Beutenbergiaceae and formed a reliable cluster with the members of the genus Serinibacter.
30785391	9	39	theme	family	1259:1264	arg1	Beutenbergiaceae					1266:1281	the family Beutenbergiaceae	1255:1281	the family Beutenbergiaceae in terms of chemotaxonomic characteristics	1255:1324	However, strain HIsM16-52T differed from the members of the genus Serinibacter and the other genera within the family Beutenbergiaceae in terms of chemotaxonomic characteristics.
30785391	3	40	theme	peptidoglycan	404:416	arg1	lysine					344:349	lysine	344:349	lysine	344:349	Strain HIsM16-52T contained both lysine and ornithine as the diagnostic diamino acids of the peptidoglycan.
30785391	3	40	theme	peptidoglycan	404:416	arg1	acids					391:395	the diagnostic diamino acids	368:395	the diagnostic diamino acids of the peptidoglycan	368:416	Strain HIsM16-52T contained both lysine and ornithine as the diagnostic diamino acids of the peptidoglycan.
30785391	3	40	theme	peptidoglycan	404:416	arg1	ornithine					355:363	ornithine	355:363	ornithine	355:363	Strain HIsM16-52T contained both lysine and ornithine as the diagnostic diamino acids of the peptidoglycan.
30785391	7	41	theme	rRNA	757:760	arg1	comparison					776:785	16S rRNA gene sequence comparison	753:785	16S rRNA gene sequence comparison	753:785	Phylogenetic analyses based on 16S rRNA gene sequence comparison revealed that strain HIsM16-52T fell within the cluster of the family Beutenbergiaceae and formed a reliable cluster with the members of the genus Serinibacter.
30785391	10	42	dep	sp	1497:1498	arg1	name					1454:1457	the name	1450:1457	the name Litorihabitans aurantiacus gen. nov.	1450:1494	Therefore, strain HIsM16-52T is concluded to represent a novel genus and species of the family Beutenbergiaceae, for which the name Litorihabitans aurantiacus gen. nov., sp.
30785391	2	43	theme	Ishigaki	216:223	arg1	Island					225:230	Ishigaki Island	216:230	Ishigaki Island in Japan	216:239	A novel actinobacterium, designated HIsM16-52T, was isolated from beach sand collected from Ishigaki Island in Japan and its taxonomic position was investigated by a polyphasic approach.
30785391	7	44	theme	gene	762:765	arg1	comparison					776:785	16S rRNA gene sequence comparison	753:785	16S rRNA gene sequence comparison	753:785	Phylogenetic analyses based on 16S rRNA gene sequence comparison revealed that strain HIsM16-52T fell within the cluster of the family Beutenbergiaceae and formed a reliable cluster with the members of the genus Serinibacter.
30785391	5	45	theme	unidentified	642:653	arg1	phospholipids					655:667	two unidentified phospholipids	638:667	two unidentified phospholipids	638:667	The detected polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol and two unidentified phospholipids.
30785391	9	46	theme	strain	1157:1162	arg1	HIsM16-52T					1164:1173	strain HIsM16-52T	1157:1173	strain HIsM16-52T	1157:1173	However, strain HIsM16-52T differed from the members of the genus Serinibacter and the other genera within the family Beutenbergiaceae in terms of chemotaxonomic characteristics.
30785391	9	47	theme	genus	1208:1212	arg1	Serinibacter					1214:1225	the genus Serinibacter	1204:1225	the genus Serinibacter	1204:1225	However, strain HIsM16-52T differed from the members of the genus Serinibacter and the other genera within the family Beutenbergiaceae in terms of chemotaxonomic characteristics.
30785391	1	48	attach	isolated	98:105	arg1	sand					118:121	beach sand	112:121	beach sand	112:121	nov., an actinobacterium of the family Beutenbergiaceae isolated from beach sand.
30785391	1	48	attach	isolated	98:105	arg2	Beutenbergiaceae					81:96	the family Beutenbergiaceae	70:96	the family Beutenbergiaceae isolated from beach sand	70:121	nov., an actinobacterium of the family Beutenbergiaceae isolated from beach sand.
30785391	10	49	dep	name	1454:1457	arg1	nov.					1491:1494	Litorihabitans aurantiacus gen. nov.	1459:1494	the name Litorihabitans aurantiacus gen. nov.	1450:1494	Therefore, strain HIsM16-52T is concluded to represent a novel genus and species of the family Beutenbergiaceae, for which the name Litorihabitans aurantiacus gen. nov., sp.
30785391	10	50	theme	strain	1338:1343	arg1	HIsM16-52T					1345:1354	strain HIsM16-52T	1338:1354	strain HIsM16-52T	1338:1354	Therefore, strain HIsM16-52T is concluded to represent a novel genus and species of the family Beutenbergiaceae, for which the name Litorihabitans aurantiacus gen. nov., sp.
30785391	1	51	theme	beach	112:116	arg1	sand					118:121	beach sand	112:121	beach sand	112:121	nov., an actinobacterium of the family Beutenbergiaceae isolated from beach sand.
30785391	7	52	theme	16S	753:755	arg1	rRNA					757:760	16S rRNA	753:760	16S rRNA gene sequence comparison	753:785	Phylogenetic analyses based on 16S rRNA gene sequence comparison revealed that strain HIsM16-52T fell within the cluster of the family Beutenbergiaceae and formed a reliable cluster with the members of the genus Serinibacter.
30785391	2	53	theme	beach	190:194	arg1	sand					196:199	beach sand	190:199	beach sand collected from Ishigaki Island in Japan	190:239	A novel actinobacterium, designated HIsM16-52T, was isolated from beach sand collected from Ishigaki Island in Japan and its taxonomic position was investigated by a polyphasic approach.
30785391	8	54	theme	gene	969:972	arg1	similarities					983:994	The highest 16S rRNA gene sequence similarities	948:994	The highest 16S rRNA gene sequence similarities	948:994	The highest 16S rRNA gene sequence similarities were obtained to species of the genus Serinibacter(97.8-97.9 %), followed by the genera Miniimonas (97.0 %), Beutenbergia (96.4 %) and Salana (95.9 %).
30785391	5	55	theme	detected	540:547	arg1	phosphatidylglycerol					567:586	phosphatidylglycerol	567:586	phosphatidylglycerol	567:586	The detected polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol and two unidentified phospholipids.
30785391	5	55	theme	detected	540:547	arg1	lipids					555:560	The detected polar lipids	536:560	The detected polar lipids	536:560	The detected polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol and two unidentified phospholipids.
30785391	9	56	theme	chemotaxonomic	1295:1308	arg1	characteristics					1310:1324	chemotaxonomic characteristics	1295:1324	chemotaxonomic characteristics	1295:1324	However, strain HIsM16-52T differed from the members of the genus Serinibacter and the other genera within the family Beutenbergiaceae in terms of chemotaxonomic characteristics.
30785391	8	57	theme	genera	1077:1082	arg1	%					1101:1101	97.0 %	1096:1101	97.0 %	1096:1101	The highest 16S rRNA gene sequence similarities were obtained to species of the genus Serinibacter(97.8-97.9 %), followed by the genera Miniimonas (97.0 %), Beutenbergia (96.4 %) and Salana (95.9 %).
30785391	8	57	theme	genera	1077:1082	arg1	Miniimonas					1084:1093	the genera Miniimonas	1073:1093	the genera Miniimonas (97.0 %)	1073:1102	The highest 16S rRNA gene sequence similarities were obtained to species of the genus Serinibacter(97.8-97.9 %), followed by the genera Miniimonas (97.0 %), Beutenbergia (96.4 %) and Salana (95.9 %).
30785391	4	58	theme	isoprenoid	435:444	arg1	MK-8					458:461	MK-8	458:461	MK-8	458:461	The predominant isoprenoid quinone was MK-8(H4) and the major fatty acids were anteiso-C15 : 0, C16 : 0 and C14 : 0.
30785391	4	58	theme	isoprenoid	435:444	arg1	quinone					446:452	The predominant isoprenoid quinone	419:452	The predominant isoprenoid quinone	419:452	The predominant isoprenoid quinone was MK-8(H4) and the major fatty acids were anteiso-C15 : 0, C16 : 0 and C14 : 0.
30785391	9	59	theme	characteristics	1310:1324	arg1	terms					1286:1290	terms	1286:1290	terms of chemotaxonomic characteristics	1286:1324	However, strain HIsM16-52T differed from the members of the genus Serinibacter and the other genera within the family Beutenbergiaceae in terms of chemotaxonomic characteristics.
30785391	10	60	theme	gen.	1486:1489	arg1	nov.					1491:1494	Litorihabitans aurantiacus gen. nov.	1459:1494	the name Litorihabitans aurantiacus gen. nov.	1450:1494	Therefore, strain HIsM16-52T is concluded to represent a novel genus and species of the family Beutenbergiaceae, for which the name Litorihabitans aurantiacus gen. nov., sp.
30785391	4	61	theme	predominant	423:433	arg1	MK-8					458:461	MK-8	458:461	MK-8	458:461	The predominant isoprenoid quinone was MK-8(H4) and the major fatty acids were anteiso-C15 : 0, C16 : 0 and C14 : 0.
30785391	4	61	theme	predominant	423:433	arg1	quinone					446:452	The predominant isoprenoid quinone	419:452	The predominant isoprenoid quinone	419:452	The predominant isoprenoid quinone was MK-8(H4) and the major fatty acids were anteiso-C15 : 0, C16 : 0 and C14 : 0.
30785391	6	62	theme	73.4 mol	711:718	arg1	G+C content					678:688	The DNA G+C content	670:688	The DNA G+C content	670:688	The DNA G+C content was determined to be 73.4 mol%.
30785391	6	62	theme	73.4 mol	711:718	arg1	%					719:719	73.4 mol%	711:719	73.4 mol%	711:719	The DNA G+C content was determined to be 73.4 mol%.
30785391	7	63	theme	reliable	887:894	arg1	cluster					896:902	a reliable cluster	885:902	a reliable cluster	885:902	Phylogenetic analyses based on 16S rRNA gene sequence comparison revealed that strain HIsM16-52T fell within the cluster of the family Beutenbergiaceae and formed a reliable cluster with the members of the genus Serinibacter.
30785391	10	64	theme	aurantiacus	1474:1484	arg1	nov.					1491:1494	Litorihabitans aurantiacus gen. nov.	1459:1494	the name Litorihabitans aurantiacus gen. nov.	1450:1494	Therefore, strain HIsM16-52T is concluded to represent a novel genus and species of the family Beutenbergiaceae, for which the name Litorihabitans aurantiacus gen. nov., sp.
30785391	4	65	theme	fatty	481:485	arg1	anteiso-C15 					498:509	anteiso-C15 	498:509	anteiso-C15 	498:509	The predominant isoprenoid quinone was MK-8(H4) and the major fatty acids were anteiso-C15 : 0, C16 : 0 and C14 : 0.
30785391	4	65	theme	fatty	481:485	arg1	acids					487:491	the major fatty acids	471:491	the major fatty acids	471:491	The predominant isoprenoid quinone was MK-8(H4) and the major fatty acids were anteiso-C15 : 0, C16 : 0 and C14 : 0.
30785391	0	66	theme	gen.	27:30	arg1	sp					38:39	sp	38:39	sp	38:39	Litorihabitans aurantiacus gen. nov., sp.
30785391	0	66	theme	gen.	27:30	arg1	nov.					32:35	Litorihabitans aurantiacus gen. nov.	0:35	Litorihabitans aurantiacus gen. nov.	0:35	Litorihabitans aurantiacus gen. nov., sp.
30785391	3	67	contain	contained	329:337	arg1	HIsM16-52T					318:327	Strain HIsM16-52T	311:327	Strain HIsM16-52T	311:327	Strain HIsM16-52T contained both lysine and ornithine as the diagnostic diamino acids of the peptidoglycan.
30785391	3	67	contain	contained	329:337	arg2	ornithine					355:363	ornithine	355:363	ornithine	355:363	Strain HIsM16-52T contained both lysine and ornithine as the diagnostic diamino acids of the peptidoglycan.
30785391	3	67	contain	contained	329:337	arg2	lysine					344:349	lysine	344:349	lysine	344:349	Strain HIsM16-52T contained both lysine and ornithine as the diagnostic diamino acids of the peptidoglycan.
30785391	3	67	contain	contained	329:337	arg2	acids					391:395	the diagnostic diamino acids	368:395	the diagnostic diamino acids of the peptidoglycan	368:416	Strain HIsM16-52T contained both lysine and ornithine as the diagnostic diamino acids of the peptidoglycan.
30785391	3	68	theme	diagnostic	372:381	arg1	lysine					344:349	lysine	344:349	lysine	344:349	Strain HIsM16-52T contained both lysine and ornithine as the diagnostic diamino acids of the peptidoglycan.
30785391	3	68	theme	diagnostic	372:381	arg1	acids					391:395	the diagnostic diamino acids	368:395	the diagnostic diamino acids of the peptidoglycan	368:416	Strain HIsM16-52T contained both lysine and ornithine as the diagnostic diamino acids of the peptidoglycan.
30785391	3	68	theme	diagnostic	372:381	arg1	ornithine					355:363	ornithine	355:363	ornithine	355:363	Strain HIsM16-52T contained both lysine and ornithine as the diagnostic diamino acids of the peptidoglycan.
30785391	7	69	theme	Phylogenetic	722:733	arg1	analyses					735:742	Phylogenetic analyses	722:742	Phylogenetic analyses based on 16S rRNA gene sequence comparison	722:785	Phylogenetic analyses based on 16S rRNA gene sequence comparison revealed that strain HIsM16-52T fell within the cluster of the family Beutenbergiaceae and formed a reliable cluster with the members of the genus Serinibacter.
31204968	6	0	theme	peptidoglycan	1067:1079	arg1	layer					1081:1085	its peptidoglycan layer	1063:1085	its peptidoglycan layer	1063:1085	Strain HST21T is characterized by the presence of ll-diaminopimelic acid in its peptidoglycan layer; glucose and ribose as whole cell sugars; diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, glycophospholipids, unknown lipids and phospholipids as polar lipids; and anteiso-C15 : 0 (21.6 %) and anteiso-C17 : 0 (20.5 %) as major fatty acids (>15 %).
31204968	2	1	theme	Atacama	312:318	arg1	Desert					320:325	the Atacama Desert	308:325	the Atacama Desert	308:325	A polyphasic approach was used for evaluating the taxonomic status of strain HST21T isolated from Salar de Huasco in the Atacama Desert.
31204968	7	2	theme	HST21T	1422:1427	arg1	merits					1429:1434	strain HST21T merits	1415:1434	strain HST21T merits	1415:1434	Based on these results, strain HST21T merits recognition as a novel species, for which the name Streptomyces altiplanensis sp.
31204968	10	3	theme	rRNA	1737:1740	arg1	similarity					1756:1765	100 % 16S rRNA gene sequence similarity	1727:1765	100 % 16S rRNA gene sequence similarity with digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values of 95.3 and 99.4 %, respectively	1727:1885	While analysing the phylogenies of strain HST21T, Streptomyces chryseus DSM 40420T and Streptomyces helvaticus DSM 40431T were found to have 100 % 16S rRNA gene sequence similarity with digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values of 95.3 and 99.4 %, respectively.
31204968	10	4	dep	Streptomyces	1636:1647	arg1	chryseus					1649:1656	chryseus	1649:1656	chryseus	1649:1656	While analysing the phylogenies of strain HST21T, Streptomyces chryseus DSM 40420T and Streptomyces helvaticus DSM 40431T were found to have 100 % 16S rRNA gene sequence similarity with digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values of 95.3 and 99.4 %, respectively.
31204968	3	5	theme	nearest	546:552	arg1	HST21T					419:424	strain HST21T	412:424	strain HST21T	412:424	The results of 16S rRNA gene and multilocus sequence phylogenetic analyses assigned strain HST21T to the genus Streptomyceswith Streptomyces albidochromogenes DSM 41800Tand Streptomyces flavidovirens DSM 40150T as its nearest neighbours.
31204968	3	5	theme	nearest	546:552	arg1	neighbours					554:563	its nearest neighbours	542:563	its nearest neighbours	542:563	The results of 16S rRNA gene and multilocus sequence phylogenetic analyses assigned strain HST21T to the genus Streptomyceswith Streptomyces albidochromogenes DSM 41800Tand Streptomyces flavidovirens DSM 40150T as its nearest neighbours.
31204968	6	6	dep	anteiso-C15 	1307:1318	arg1	 0					1320:1321	 0	1320:1321	 0	1320:1321	Strain HST21T is characterized by the presence of ll-diaminopimelic acid in its peptidoglycan layer; glucose and ribose as whole cell sugars; diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, glycophospholipids, unknown lipids and phospholipids as polar lipids; and anteiso-C15 : 0 (21.6 %) and anteiso-C17 : 0 (20.5 %) as major fatty acids (>15 %).
31204968	6	6	dep	anteiso-C15 	1307:1318	arg1	%					1329:1329	21.6 %	1324:1329	21.6 %	1324:1329	Strain HST21T is characterized by the presence of ll-diaminopimelic acid in its peptidoglycan layer; glucose and ribose as whole cell sugars; diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, glycophospholipids, unknown lipids and phospholipids as polar lipids; and anteiso-C15 : 0 (21.6 %) and anteiso-C17 : 0 (20.5 %) as major fatty acids (>15 %).
31204968	6	6	dep	anteiso-C15 	1307:1318	arg1	 0					1349:1350	 0	1349:1350	 0	1349:1350	Strain HST21T is characterized by the presence of ll-diaminopimelic acid in its peptidoglycan layer; glucose and ribose as whole cell sugars; diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, glycophospholipids, unknown lipids and phospholipids as polar lipids; and anteiso-C15 : 0 (21.6 %) and anteiso-C17 : 0 (20.5 %) as major fatty acids (>15 %).
31204968	6	6	dep	anteiso-C15 	1307:1318	arg1	%					1358:1358	20.5 %	1353:1358	20.5 %	1353:1358	Strain HST21T is characterized by the presence of ll-diaminopimelic acid in its peptidoglycan layer; glucose and ribose as whole cell sugars; diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, glycophospholipids, unknown lipids and phospholipids as polar lipids; and anteiso-C15 : 0 (21.6 %) and anteiso-C17 : 0 (20.5 %) as major fatty acids (>15 %).
31204968	6	6	dep	anteiso-C15 	1307:1318	arg1	anteiso-C17 					1336:1347	anteiso-C17 	1336:1347	anteiso-C17 	1336:1347	Strain HST21T is characterized by the presence of ll-diaminopimelic acid in its peptidoglycan layer; glucose and ribose as whole cell sugars; diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, glycophospholipids, unknown lipids and phospholipids as polar lipids; and anteiso-C15 : 0 (21.6 %) and anteiso-C17 : 0 (20.5 %) as major fatty acids (>15 %).
31204968	2	7	theme	taxonomic	241:249	arg1	status					251:256	the taxonomic status	237:256	the taxonomic status of strain HST21T isolated from Salar de Huasco in the Atacama Desert	237:325	A polyphasic approach was used for evaluating the taxonomic status of strain HST21T isolated from Salar de Huasco in the Atacama Desert.
31204968	11	8	theme	chryseus	2023:2030	arg1	synonym					1950:1956	a later heterotypic synonym	1930:1956	a later heterotypic synonym of S. chryseus	1930:1971	Therefore, S. helvaticus is considered as a later heterotypic synonym of S. chryseus and, consequently, we emend the description of S. chryseus.
31204968	11	8	theme	chryseus	2023:2030	arg1	description					2005:2015	the description	2001:2015	the description of S. chryseus	2001:2030	Therefore, S. helvaticus is considered as a later heterotypic synonym of S. chryseus and, consequently, we emend the description of S. chryseus.
31204968	11	8	theme	chryseus	2023:2030	arg1	helvaticus					1902:1911	S. helvaticus	1899:1911	S. helvaticus	1899:1911	Therefore, S. helvaticus is considered as a later heterotypic synonym of S. chryseus and, consequently, we emend the description of S. chryseus.
31204968	1	9	attach	isolated	63:70	arg2	species					55:61	an alkalitolerant species	37:61	an alkalitolerant species isolated from Chilean Altiplano soil	37:98	nov., an alkalitolerant species isolated from Chilean Altiplano soil, and emended description of Streptomyces chryseus (Krasil'nikov et al. 1965) Pridham 1970.
31204968	1	9	attach	isolated	63:70	arg1	soil					95:98	Chilean Altiplano soil	77:98	Chilean Altiplano soil	77:98	nov., an alkalitolerant species isolated from Chilean Altiplano soil, and emended description of Streptomyces chryseus (Krasil'nikov et al. 1965) Pridham 1970.
31204968	6	10	from	presence	1025:1032	arg1	layer					1081:1085	its peptidoglycan layer	1063:1085	its peptidoglycan layer	1063:1085	Strain HST21T is characterized by the presence of ll-diaminopimelic acid in its peptidoglycan layer; glucose and ribose as whole cell sugars; diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, glycophospholipids, unknown lipids and phospholipids as polar lipids; and anteiso-C15 : 0 (21.6 %) and anteiso-C17 : 0 (20.5 %) as major fatty acids (>15 %).
31204968	10	11	theme	100 	1727:1730	arg1	%					1731:1731	%	1731:1731	%	1731:1731	While analysing the phylogenies of strain HST21T, Streptomyces chryseus DSM 40420T and Streptomyces helvaticus DSM 40431T were found to have 100 % 16S rRNA gene sequence similarity with digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values of 95.3 and 99.4 %, respectively.
31204968	6	12	theme	cell	1116:1119	arg1	sugars					1121:1126	whole cell sugars	1110:1126	whole cell sugars	1110:1126	Strain HST21T is characterized by the presence of ll-diaminopimelic acid in its peptidoglycan layer; glucose and ribose as whole cell sugars; diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, glycophospholipids, unknown lipids and phospholipids as polar lipids; and anteiso-C15 : 0 (21.6 %) and anteiso-C17 : 0 (20.5 %) as major fatty acids (>15 %).
31204968	2	13	theme	HST21T	268:273	arg1	status					251:256	the taxonomic status	237:256	the taxonomic status of strain HST21T isolated from Salar de Huasco in the Atacama Desert	237:325	A polyphasic approach was used for evaluating the taxonomic status of strain HST21T isolated from Salar de Huasco in the Atacama Desert.
31204968	2	14	attach	isolated	275:282	arg2	HST21T					268:273	strain HST21T	261:273	strain HST21T isolated from Salar de Huasco in the Atacama Desert	261:325	A polyphasic approach was used for evaluating the taxonomic status of strain HST21T isolated from Salar de Huasco in the Atacama Desert.
31204968	2	14	attach	isolated	275:282	arg1	Salar					289:293	Salar	289:293	Salar	289:293	A polyphasic approach was used for evaluating the taxonomic status of strain HST21T isolated from Salar de Huasco in the Atacama Desert.
31204968	6	15	theme	lipids	1261:1266	arg1	presence					1025:1032	the presence	1021:1032	the presence of ll-diaminopimelic acid in its peptidoglycan layer; glucose and ribose as whole cell sugars; diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, glycophospholipids, unknown lipids and phospholipids as polar lipids; and anteiso-C15 : 0 (21.6 %) and anteiso-C17 : 0 (20.5 %) as major fatty acids (>15 %)	1021:1388	Strain HST21T is characterized by the presence of ll-diaminopimelic acid in its peptidoglycan layer; glucose and ribose as whole cell sugars; diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, glycophospholipids, unknown lipids and phospholipids as polar lipids; and anteiso-C15 : 0 (21.6 %) and anteiso-C17 : 0 (20.5 %) as major fatty acids (>15 %).
31204968	10	16	theme	gene	1742:1745	arg1	similarity					1756:1765	100 % 16S rRNA gene sequence similarity	1727:1765	100 % 16S rRNA gene sequence similarity with digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values of 95.3 and 99.4 %, respectively	1727:1885	While analysing the phylogenies of strain HST21T, Streptomyces chryseus DSM 40420T and Streptomyces helvaticus DSM 40431T were found to have 100 % 16S rRNA gene sequence similarity with digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values of 95.3 and 99.4 %, respectively.
31204968	10	17	with	similarity	1756:1765	arg1	values					1847:1852	digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values	1772:1852	digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values	1772:1852	While analysing the phylogenies of strain HST21T, Streptomyces chryseus DSM 40420T and Streptomyces helvaticus DSM 40431T were found to have 100 % 16S rRNA gene sequence similarity with digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values of 95.3 and 99.4 %, respectively.
31204968	4	18	theme	%	840:840	arg1	thresholds					813:822	the thresholds	809:822	the thresholds of 70 and 95-96 % for prokaryotic conspecific assignation	809:880	Digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values between the genome sequences of strain HST21T and S. albidochromogenes DSM 41800T (35.6 and 88.2 %) and S. flavidovirens DSM 40105T (47.2 and 88.8 %) were below the thresholds of 70 and 95-96 % for prokaryotic conspecific assignation.
31204968	4	19	theme	albidochromogenes	701:717	arg1	41800T					723:728	S. albidochromogenes DSM 41800T	698:728	S. albidochromogenes DSM 41800T	698:728	Digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values between the genome sequences of strain HST21T and S. albidochromogenes DSM 41800T (35.6 and 88.2 %) and S. flavidovirens DSM 40105T (47.2 and 88.8 %) were below the thresholds of 70 and 95-96 % for prokaryotic conspecific assignation.
31204968	10	20	theme	16S	1733:1735	arg1	similarity					1756:1765	100 % 16S rRNA gene sequence similarity	1727:1765	100 % 16S rRNA gene sequence similarity with digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values of 95.3 and 99.4 %, respectively	1727:1885	While analysing the phylogenies of strain HST21T, Streptomyces chryseus DSM 40420T and Streptomyces helvaticus DSM 40431T were found to have 100 % 16S rRNA gene sequence similarity with digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values of 95.3 and 99.4 %, respectively.
31204968	4	21	theme	prokaryotic	846:856	arg1	assignation					870:880	prokaryotic conspecific assignation	846:880	prokaryotic conspecific assignation	846:880	Digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values between the genome sequences of strain HST21T and S. albidochromogenes DSM 41800T (35.6 and 88.2 %) and S. flavidovirens DSM 40105T (47.2 and 88.8 %) were below the thresholds of 70 and 95-96 % for prokaryotic conspecific assignation.
31204968	2	22	dep	Salar	289:293	arg1	de					295:296	Salar de Huasco	289:303	Salar de Huasco in the Atacama Desert	289:325	A polyphasic approach was used for evaluating the taxonomic status of strain HST21T isolated from Salar de Huasco in the Atacama Desert.
31204968	2	22	dep	Salar	289:293	arg1	Huasco					298:303	Huasco	298:303	Huasco	298:303	A polyphasic approach was used for evaluating the taxonomic status of strain HST21T isolated from Salar de Huasco in the Atacama Desert.
31204968	6	23	from	glycophospholipids	1233:1250	arg1	layer					1081:1085	its peptidoglycan layer	1063:1085	its peptidoglycan layer	1063:1085	Strain HST21T is characterized by the presence of ll-diaminopimelic acid in its peptidoglycan layer; glucose and ribose as whole cell sugars; diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, glycophospholipids, unknown lipids and phospholipids as polar lipids; and anteiso-C15 : 0 (21.6 %) and anteiso-C17 : 0 (20.5 %) as major fatty acids (>15 %).
31204968	10	24	theme	digital	1772:1778	arg1	dDDH					1803:1806	dDDH	1803:1806	dDDH	1803:1806	While analysing the phylogenies of strain HST21T, Streptomyces chryseus DSM 40420T and Streptomyces helvaticus DSM 40431T were found to have 100 % 16S rRNA gene sequence similarity with digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values of 95.3 and 99.4 %, respectively.
31204968	10	24	theme	digital	1772:1778	arg1	hydridization					1788:1800	digital DNA-DNA hydridization	1772:1800	digital DNA-DNA hydridization (dDDH)	1772:1807	While analysing the phylogenies of strain HST21T, Streptomyces chryseus DSM 40420T and Streptomyces helvaticus DSM 40431T were found to have 100 % 16S rRNA gene sequence similarity with digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values of 95.3 and 99.4 %, respectively.
31204968	5	25	theme	Phenotypic	883:892	arg1	results					922:928	Phenotypic, chemotaxonomic and genetic results	883:928	Phenotypic, chemotaxonomic and genetic results	883:928	Phenotypic, chemotaxonomic and genetic results distinguished strain HST21T from its closest neighbours.
31204968	4	26	theme	41800T	723:728	arg1	sequences					667:675	the genome sequences	656:675	the genome sequences of strain HST21T and S. albidochromogenes DSM 41800T (35.6 and 88.2 %) and S. flavidovirens DSM 40105T (47.2 and 88.8 %)	656:796	Digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values between the genome sequences of strain HST21T and S. albidochromogenes DSM 41800T (35.6 and 88.2 %) and S. flavidovirens DSM 40105T (47.2 and 88.8 %) were below the thresholds of 70 and 95-96 % for prokaryotic conspecific assignation.
31204968	4	27	theme	strain	680:685	arg1	HST21T					687:692	strain HST21T	680:692	strain HST21T	680:692	Digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values between the genome sequences of strain HST21T and S. albidochromogenes DSM 41800T (35.6 and 88.2 %) and S. flavidovirens DSM 40105T (47.2 and 88.8 %) were below the thresholds of 70 and 95-96 % for prokaryotic conspecific assignation.
31204968	4	27	theme	strain	680:685	arg1	%					745:745	35.6 and 88.2 %	731:745	35.6 and 88.2 %	731:745	Digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values between the genome sequences of strain HST21T and S. albidochromogenes DSM 41800T (35.6 and 88.2 %) and S. flavidovirens DSM 40105T (47.2 and 88.8 %) were below the thresholds of 70 and 95-96 % for prokaryotic conspecific assignation.
31204968	6	28	from	phosphatidylethanolamine	1185:1208	arg1	layer					1081:1085	its peptidoglycan layer	1063:1085	its peptidoglycan layer	1063:1085	Strain HST21T is characterized by the presence of ll-diaminopimelic acid in its peptidoglycan layer; glucose and ribose as whole cell sugars; diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, glycophospholipids, unknown lipids and phospholipids as polar lipids; and anteiso-C15 : 0 (21.6 %) and anteiso-C17 : 0 (20.5 %) as major fatty acids (>15 %).
31204968	6	29	from	phosphatidylinositol	1211:1230	arg1	layer					1081:1085	its peptidoglycan layer	1063:1085	its peptidoglycan layer	1063:1085	Strain HST21T is characterized by the presence of ll-diaminopimelic acid in its peptidoglycan layer; glucose and ribose as whole cell sugars; diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, glycophospholipids, unknown lipids and phospholipids as polar lipids; and anteiso-C15 : 0 (21.6 %) and anteiso-C17 : 0 (20.5 %) as major fatty acids (>15 %).
31204968	7	30	theme	Streptomyces	1487:1498	arg1	sp					1514:1515	the name Streptomyces altiplanensis sp	1478:1515	the name Streptomyces altiplanensis sp	1478:1515	Based on these results, strain HST21T merits recognition as a novel species, for which the name Streptomyces altiplanensis sp.
31204968	10	31	theme	strain	1621:1626	arg1	HST21T					1628:1633	strain HST21T	1621:1633	strain HST21T	1621:1633	While analysing the phylogenies of strain HST21T, Streptomyces chryseus DSM 40420T and Streptomyces helvaticus DSM 40431T were found to have 100 % 16S rRNA gene sequence similarity with digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values of 95.3 and 99.4 %, respectively.
31204968	4	32	theme	S.	752:753	arg1	40105T					773:778	S. flavidovirens DSM 40105T	752:778	S. flavidovirens DSM 40105T (47.2 and 88.8 %)	752:796	Digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values between the genome sequences of strain HST21T and S. albidochromogenes DSM 41800T (35.6 and 88.2 %) and S. flavidovirens DSM 40105T (47.2 and 88.8 %) were below the thresholds of 70 and 95-96 % for prokaryotic conspecific assignation.
31204968	4	32	theme	S.	752:753	arg1	%					795:795	47.2 and 88.8 %	781:795	%	795:795	Digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values between the genome sequences of strain HST21T and S. albidochromogenes DSM 41800T (35.6 and 88.2 %) and S. flavidovirens DSM 40105T (47.2 and 88.8 %) were below the thresholds of 70 and 95-96 % for prokaryotic conspecific assignation.
31204968	6	33	from	diphosphatidylglycerol	1129:1150	arg1	layer					1081:1085	its peptidoglycan layer	1063:1085	its peptidoglycan layer	1063:1085	Strain HST21T is characterized by the presence of ll-diaminopimelic acid in its peptidoglycan layer; glucose and ribose as whole cell sugars; diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, glycophospholipids, unknown lipids and phospholipids as polar lipids; and anteiso-C15 : 0 (21.6 %) and anteiso-C17 : 0 (20.5 %) as major fatty acids (>15 %).
31204968	1	34	theme	Chilean	77:83	arg1	soil					95:98	Chilean Altiplano soil	77:98	Chilean Altiplano soil	77:98	nov., an alkalitolerant species isolated from Chilean Altiplano soil, and emended description of Streptomyces chryseus (Krasil'nikov et al. 1965) Pridham 1970.
31204968	6	35	theme	acid	1055:1058	arg1	presence					1025:1032	the presence	1021:1032	the presence of ll-diaminopimelic acid in its peptidoglycan layer; glucose and ribose as whole cell sugars; diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, glycophospholipids, unknown lipids and phospholipids as polar lipids; and anteiso-C15 : 0 (21.6 %) and anteiso-C17 : 0 (20.5 %) as major fatty acids (>15 %)	1021:1388	Strain HST21T is characterized by the presence of ll-diaminopimelic acid in its peptidoglycan layer; glucose and ribose as whole cell sugars; diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, glycophospholipids, unknown lipids and phospholipids as polar lipids; and anteiso-C15 : 0 (21.6 %) and anteiso-C17 : 0 (20.5 %) as major fatty acids (>15 %).
31204968	3	36	theme	16S	343:345	arg1	rRNA					347:350	16S rRNA	343:350	16S rRNA gene	343:355	The results of 16S rRNA gene and multilocus sequence phylogenetic analyses assigned strain HST21T to the genus Streptomyceswith Streptomyces albidochromogenes DSM 41800Tand Streptomyces flavidovirens DSM 40150T as its nearest neighbours.
31204968	1	37	dep	Krasil	151:156	arg1	nikov					158:162	nikov	158:162	nikov	158:162	nov., an alkalitolerant species isolated from Chilean Altiplano soil, and emended description of Streptomyces chryseus (Krasil'nikov et al. 1965) Pridham 1970.
31204968	6	38	from	phosphatidylmethylethanolamine	1153:1182	arg1	layer					1081:1085	its peptidoglycan layer	1063:1085	its peptidoglycan layer	1063:1085	Strain HST21T is characterized by the presence of ll-diaminopimelic acid in its peptidoglycan layer; glucose and ribose as whole cell sugars; diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, glycophospholipids, unknown lipids and phospholipids as polar lipids; and anteiso-C15 : 0 (21.6 %) and anteiso-C17 : 0 (20.5 %) as major fatty acids (>15 %).
31204968	3	39	theme	phylogenetic	381:392	arg1	analyses					394:401	multilocus sequence phylogenetic analyses	361:401	multilocus sequence phylogenetic analyses	361:401	The results of 16S rRNA gene and multilocus sequence phylogenetic analyses assigned strain HST21T to the genus Streptomyceswith Streptomyces albidochromogenes DSM 41800Tand Streptomyces flavidovirens DSM 40150T as its nearest neighbours.
31204968	0	40	theme	Streptomyces	0:11	arg1	sp					27:28	Streptomyces altiplanensis sp	0:28	Streptomyces altiplanensis sp.	0:29	Streptomyces altiplanensis sp.
31204968	5	41	theme	chemotaxonomic	895:908	arg1	results					922:928	Phenotypic, chemotaxonomic and genetic results	883:928	Phenotypic, chemotaxonomic and genetic results	883:928	Phenotypic, chemotaxonomic and genetic results distinguished strain HST21T from its closest neighbours.
31204968	1	42	dep	chryseus	141:148	arg1	Krasil					151:156	Krasil	151:156	Krasil	151:156	nov., an alkalitolerant species isolated from Chilean Altiplano soil, and emended description of Streptomyces chryseus (Krasil'nikov et al. 1965) Pridham 1970.
31204968	10	43	theme	HST21T	1628:1633	arg1	phylogenies					1606:1616	the phylogenies	1602:1616	the phylogenies of strain HST21T	1602:1633	While analysing the phylogenies of strain HST21T, Streptomyces chryseus DSM 40420T and Streptomyces helvaticus DSM 40431T were found to have 100 % 16S rRNA gene sequence similarity with digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values of 95.3 and 99.4 %, respectively.
31204968	5	44	theme	genetic	914:920	arg1	results					922:928	Phenotypic, chemotaxonomic and genetic results	883:928	Phenotypic, chemotaxonomic and genetic results	883:928	Phenotypic, chemotaxonomic and genetic results distinguished strain HST21T from its closest neighbours.
31204968	3	45	theme	multilocus	361:370	arg1	analyses					394:401	multilocus sequence phylogenetic analyses	361:401	multilocus sequence phylogenetic analyses	361:401	The results of 16S rRNA gene and multilocus sequence phylogenetic analyses assigned strain HST21T to the genus Streptomyceswith Streptomyces albidochromogenes DSM 41800Tand Streptomyces flavidovirens DSM 40150T as its nearest neighbours.
31204968	4	46	theme	Digital	566:572	arg1	dDDH					597:600	dDDH	597:600	dDDH	597:600	Digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values between the genome sequences of strain HST21T and S. albidochromogenes DSM 41800T (35.6 and 88.2 %) and S. flavidovirens DSM 40105T (47.2 and 88.8 %) were below the thresholds of 70 and 95-96 % for prokaryotic conspecific assignation.
31204968	4	46	theme	Digital	566:572	arg1	hydridization					582:594	Digital DNA-DNA hydridization	566:594	Digital DNA-DNA hydridization (dDDH)	566:601	Digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values between the genome sequences of strain HST21T and S. albidochromogenes DSM 41800T (35.6 and 88.2 %) and S. flavidovirens DSM 40105T (47.2 and 88.8 %) were below the thresholds of 70 and 95-96 % for prokaryotic conspecific assignation.
31204968	3	47	theme	gene	352:355	arg1	results					332:338	The results	328:338	The results of 16S rRNA gene and multilocus sequence phylogenetic analyses	328:401	The results of 16S rRNA gene and multilocus sequence phylogenetic analyses assigned strain HST21T to the genus Streptomyceswith Streptomyces albidochromogenes DSM 41800Tand Streptomyces flavidovirens DSM 40150T as its nearest neighbours.
31204968	10	48	theme	hydridization	1788:1800	arg1	values					1847:1852	digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values	1772:1852	digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values	1772:1852	While analysing the phylogenies of strain HST21T, Streptomyces chryseus DSM 40420T and Streptomyces helvaticus DSM 40431T were found to have 100 % 16S rRNA gene sequence similarity with digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values of 95.3 and 99.4 %, respectively.
31204968	6	49	from	glucose	1088:1094	arg1	layer					1081:1085	its peptidoglycan layer	1063:1085	its peptidoglycan layer	1063:1085	Strain HST21T is characterized by the presence of ll-diaminopimelic acid in its peptidoglycan layer; glucose and ribose as whole cell sugars; diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, glycophospholipids, unknown lipids and phospholipids as polar lipids; and anteiso-C15 : 0 (21.6 %) and anteiso-C17 : 0 (20.5 %) as major fatty acids (>15 %).
31204968	10	50	theme	DSM	1658:1660	arg1	40420T					1662:1667	Streptomyces chryseus DSM 40420T	1636:1667	Streptomyces chryseus DSM 40420T	1636:1667	While analysing the phylogenies of strain HST21T, Streptomyces chryseus DSM 40420T and Streptomyces helvaticus DSM 40431T were found to have 100 % 16S rRNA gene sequence similarity with digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values of 95.3 and 99.4 %, respectively.
31204968	6	51	theme	fatty	1370:1374	arg1	acids					1376:1380	major fatty acids	1364:1380	major fatty acids (>15 %)	1364:1388	Strain HST21T is characterized by the presence of ll-diaminopimelic acid in its peptidoglycan layer; glucose and ribose as whole cell sugars; diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, glycophospholipids, unknown lipids and phospholipids as polar lipids; and anteiso-C15 : 0 (21.6 %) and anteiso-C17 : 0 (20.5 %) as major fatty acids (>15 %).
31204968	6	51	theme	fatty	1370:1374	arg1	%					1387:1387	>15 %	1383:1387	>15 %	1383:1387	Strain HST21T is characterized by the presence of ll-diaminopimelic acid in its peptidoglycan layer; glucose and ribose as whole cell sugars; diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, glycophospholipids, unknown lipids and phospholipids as polar lipids; and anteiso-C15 : 0 (21.6 %) and anteiso-C17 : 0 (20.5 %) as major fatty acids (>15 %).
31204968	7	52	theme	novel	1453:1457	arg1	species					1459:1465	a novel species	1451:1465	a novel species	1451:1465	Based on these results, strain HST21T merits recognition as a novel species, for which the name Streptomyces altiplanensis sp.
31204968	10	53	theme	nucleotide	1821:1830	arg1	ANI					1842:1844	ANI	1842:1844	ANI	1842:1844	While analysing the phylogenies of strain HST21T, Streptomyces chryseus DSM 40420T and Streptomyces helvaticus DSM 40431T were found to have 100 % 16S rRNA gene sequence similarity with digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values of 95.3 and 99.4 %, respectively.
31204968	10	53	theme	nucleotide	1821:1830	arg1	identity					1832:1839	average nucleotide identity	1813:1839	average nucleotide identity (ANI)	1813:1845	While analysing the phylogenies of strain HST21T, Streptomyces chryseus DSM 40420T and Streptomyces helvaticus DSM 40431T were found to have 100 % 16S rRNA gene sequence similarity with digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values of 95.3 and 99.4 %, respectively.
31204968	4	54	theme	DSM	769:771	arg1	40105T					773:778	S. flavidovirens DSM 40105T	752:778	S. flavidovirens DSM 40105T (47.2 and 88.8 %)	752:796	Digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values between the genome sequences of strain HST21T and S. albidochromogenes DSM 41800T (35.6 and 88.2 %) and S. flavidovirens DSM 40105T (47.2 and 88.8 %) were below the thresholds of 70 and 95-96 % for prokaryotic conspecific assignation.
31204968	4	54	theme	DSM	769:771	arg1	%					795:795	47.2 and 88.8 %	781:795	%	795:795	Digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values between the genome sequences of strain HST21T and S. albidochromogenes DSM 41800T (35.6 and 88.2 %) and S. flavidovirens DSM 40105T (47.2 and 88.8 %) were below the thresholds of 70 and 95-96 % for prokaryotic conspecific assignation.
31204968	6	55	from	ribose	1100:1105	arg1	layer					1081:1085	its peptidoglycan layer	1063:1085	its peptidoglycan layer	1063:1085	Strain HST21T is characterized by the presence of ll-diaminopimelic acid in its peptidoglycan layer; glucose and ribose as whole cell sugars; diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, glycophospholipids, unknown lipids and phospholipids as polar lipids; and anteiso-C15 : 0 (21.6 %) and anteiso-C17 : 0 (20.5 %) as major fatty acids (>15 %).
31204968	10	56	theme	Streptomyces	1636:1647	arg1	40420T					1662:1667	Streptomyces chryseus DSM 40420T	1636:1667	Streptomyces chryseus DSM 40420T	1636:1667	While analysing the phylogenies of strain HST21T, Streptomyces chryseus DSM 40420T and Streptomyces helvaticus DSM 40431T were found to have 100 % 16S rRNA gene sequence similarity with digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values of 95.3 and 99.4 %, respectively.
31204968	10	57	theme	DSM	1697:1699	arg1	40431T					1701:1706	Streptomyces helvaticus DSM 40431T	1673:1706	Streptomyces helvaticus DSM 40431T	1673:1706	While analysing the phylogenies of strain HST21T, Streptomyces chryseus DSM 40420T and Streptomyces helvaticus DSM 40431T were found to have 100 % 16S rRNA gene sequence similarity with digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values of 95.3 and 99.4 %, respectively.
31204968	9	58	theme	HST21T=DSM	1555:1564	arg1	strain					1545:1550	The type strain	1536:1550	The type strain	1536:1550	The type strain is HST21T=DSM 107267T=CECT 9647T.
31204968	9	58	theme	HST21T=DSM	1555:1564	arg1	9647T					1579:1583	HST21T=DSM 107267T=CECT 9647T	1555:1583	HST21T=DSM 107267T=CECT 9647T	1555:1583	The type strain is HST21T=DSM 107267T=CECT 9647T.
31204968	6	59	theme	glucose	1088:1094	arg1	presence					1025:1032	the presence	1021:1032	the presence of ll-diaminopimelic acid in its peptidoglycan layer; glucose and ribose as whole cell sugars; diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, glycophospholipids, unknown lipids and phospholipids as polar lipids; and anteiso-C15 : 0 (21.6 %) and anteiso-C17 : 0 (20.5 %) as major fatty acids (>15 %)	1021:1388	Strain HST21T is characterized by the presence of ll-diaminopimelic acid in its peptidoglycan layer; glucose and ribose as whole cell sugars; diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, glycophospholipids, unknown lipids and phospholipids as polar lipids; and anteiso-C15 : 0 (21.6 %) and anteiso-C17 : 0 (20.5 %) as major fatty acids (>15 %).
31204968	3	60	theme	flavidovirens	514:526	arg1	40150T					532:537	the genus Streptomyceswith Streptomyces albidochromogenes DSM 41800Tand Streptomyces flavidovirens DSM 40150T	429:537	the genus Streptomyceswith Streptomyces albidochromogenes DSM 41800Tand Streptomyces flavidovirens DSM 40150T	429:537	The results of 16S rRNA gene and multilocus sequence phylogenetic analyses assigned strain HST21T to the genus Streptomyceswith Streptomyces albidochromogenes DSM 41800Tand Streptomyces flavidovirens DSM 40150T as its nearest neighbours.
31204968	6	61	from	anteiso-C15 	1307:1318	arg1	layer					1081:1085	its peptidoglycan layer	1063:1085	its peptidoglycan layer	1063:1085	Strain HST21T is characterized by the presence of ll-diaminopimelic acid in its peptidoglycan layer; glucose and ribose as whole cell sugars; diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, glycophospholipids, unknown lipids and phospholipids as polar lipids; and anteiso-C15 : 0 (21.6 %) and anteiso-C17 : 0 (20.5 %) as major fatty acids (>15 %).
31204968	1	62	theme	alkalitolerant	40:53	arg1	species					55:61	an alkalitolerant species	37:61	an alkalitolerant species isolated from Chilean Altiplano soil	37:98	nov., an alkalitolerant species isolated from Chilean Altiplano soil, and emended description of Streptomyces chryseus (Krasil'nikov et al. 1965) Pridham 1970.
31204968	4	63	theme	nucleotide	615:624	arg1	ANI					636:638	ANI	636:638	ANI	636:638	Digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values between the genome sequences of strain HST21T and S. albidochromogenes DSM 41800T (35.6 and 88.2 %) and S. flavidovirens DSM 40105T (47.2 and 88.8 %) were below the thresholds of 70 and 95-96 % for prokaryotic conspecific assignation.
31204968	4	63	theme	nucleotide	615:624	arg1	identity					626:633	average nucleotide identity	607:633	average nucleotide identity (ANI)	607:639	Digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values between the genome sequences of strain HST21T and S. albidochromogenes DSM 41800T (35.6 and 88.2 %) and S. flavidovirens DSM 40105T (47.2 and 88.8 %) were below the thresholds of 70 and 95-96 % for prokaryotic conspecific assignation.
31204968	6	64	theme	anteiso-C15 	1307:1318	arg1	presence					1025:1032	the presence	1021:1032	the presence of ll-diaminopimelic acid in its peptidoglycan layer; glucose and ribose as whole cell sugars; diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, glycophospholipids, unknown lipids and phospholipids as polar lipids; and anteiso-C15 : 0 (21.6 %) and anteiso-C17 : 0 (20.5 %) as major fatty acids (>15 %)	1021:1388	Strain HST21T is characterized by the presence of ll-diaminopimelic acid in its peptidoglycan layer; glucose and ribose as whole cell sugars; diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, glycophospholipids, unknown lipids and phospholipids as polar lipids; and anteiso-C15 : 0 (21.6 %) and anteiso-C17 : 0 (20.5 %) as major fatty acids (>15 %).
31204968	6	65	from	layer	1081:1085	arg1	presence					1025:1032	the presence	1021:1032	the presence of ll-diaminopimelic acid in its peptidoglycan layer; glucose and ribose as whole cell sugars; diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, glycophospholipids, unknown lipids and phospholipids as polar lipids; and anteiso-C15 : 0 (21.6 %) and anteiso-C17 : 0 (20.5 %) as major fatty acids (>15 %)	1021:1388	Strain HST21T is characterized by the presence of ll-diaminopimelic acid in its peptidoglycan layer; glucose and ribose as whole cell sugars; diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, glycophospholipids, unknown lipids and phospholipids as polar lipids; and anteiso-C15 : 0 (21.6 %) and anteiso-C17 : 0 (20.5 %) as major fatty acids (>15 %).
31204968	6	66	from	phospholipids	1272:1284	arg1	layer					1081:1085	its peptidoglycan layer	1063:1085	its peptidoglycan layer	1063:1085	Strain HST21T is characterized by the presence of ll-diaminopimelic acid in its peptidoglycan layer; glucose and ribose as whole cell sugars; diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, glycophospholipids, unknown lipids and phospholipids as polar lipids; and anteiso-C15 : 0 (21.6 %) and anteiso-C17 : 0 (20.5 %) as major fatty acids (>15 %).
31204968	7	67	theme	strain	1415:1420	arg1	merits					1429:1434	strain HST21T merits	1415:1434	strain HST21T merits	1415:1434	Based on these results, strain HST21T merits recognition as a novel species, for which the name Streptomyces altiplanensis sp.
31204968	4	68	theme	hydridization	582:594	arg1	values					641:646	Digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values	566:646	Digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values between the genome sequences of strain HST21T and S. albidochromogenes DSM 41800T (35.6 and 88.2 %) and S. flavidovirens DSM 40105T (47.2 and 88.8 %)	566:796	Digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values between the genome sequences of strain HST21T and S. albidochromogenes DSM 41800T (35.6 and 88.2 %) and S. flavidovirens DSM 40105T (47.2 and 88.8 %) were below the thresholds of 70 and 95-96 % for prokaryotic conspecific assignation.
31204968	2	69	theme	strain	261:266	arg1	HST21T					268:273	strain HST21T	261:273	strain HST21T isolated from Salar de Huasco in the Atacama Desert	261:325	A polyphasic approach was used for evaluating the taxonomic status of strain HST21T isolated from Salar de Huasco in the Atacama Desert.
31204968	6	70	theme	phosphatidylmethylethanolamine	1153:1182	arg1	presence					1025:1032	the presence	1021:1032	the presence of ll-diaminopimelic acid in its peptidoglycan layer; glucose and ribose as whole cell sugars; diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, glycophospholipids, unknown lipids and phospholipids as polar lipids; and anteiso-C15 : 0 (21.6 %) and anteiso-C17 : 0 (20.5 %) as major fatty acids (>15 %)	1021:1388	Strain HST21T is characterized by the presence of ll-diaminopimelic acid in its peptidoglycan layer; glucose and ribose as whole cell sugars; diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, glycophospholipids, unknown lipids and phospholipids as polar lipids; and anteiso-C15 : 0 (21.6 %) and anteiso-C17 : 0 (20.5 %) as major fatty acids (>15 %).
31204968	2	71	used	used	217:220	arg2	approach					204:211	A polyphasic approach	191:211	A polyphasic approach	191:211	A polyphasic approach was used for evaluating the taxonomic status of strain HST21T isolated from Salar de Huasco in the Atacama Desert.
31204968	6	72	theme	polar	1289:1293	arg1	lipids					1295:1300	polar lipids	1289:1300	polar lipids	1289:1300	Strain HST21T is characterized by the presence of ll-diaminopimelic acid in its peptidoglycan layer; glucose and ribose as whole cell sugars; diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, glycophospholipids, unknown lipids and phospholipids as polar lipids; and anteiso-C15 : 0 (21.6 %) and anteiso-C17 : 0 (20.5 %) as major fatty acids (>15 %).
31204968	6	73	from	acid	1055:1058	arg1	layer					1081:1085	its peptidoglycan layer	1063:1085	its peptidoglycan layer	1063:1085	Strain HST21T is characterized by the presence of ll-diaminopimelic acid in its peptidoglycan layer; glucose and ribose as whole cell sugars; diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, glycophospholipids, unknown lipids and phospholipids as polar lipids; and anteiso-C15 : 0 (21.6 %) and anteiso-C17 : 0 (20.5 %) as major fatty acids (>15 %).
31204968	6	74	theme	diphosphatidylglycerol	1129:1150	arg1	presence					1025:1032	the presence	1021:1032	the presence of ll-diaminopimelic acid in its peptidoglycan layer; glucose and ribose as whole cell sugars; diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, glycophospholipids, unknown lipids and phospholipids as polar lipids; and anteiso-C15 : 0 (21.6 %) and anteiso-C17 : 0 (20.5 %) as major fatty acids (>15 %)	1021:1388	Strain HST21T is characterized by the presence of ll-diaminopimelic acid in its peptidoglycan layer; glucose and ribose as whole cell sugars; diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, glycophospholipids, unknown lipids and phospholipids as polar lipids; and anteiso-C15 : 0 (21.6 %) and anteiso-C17 : 0 (20.5 %) as major fatty acids (>15 %).
31204968	10	75	theme	%	1731:1731	arg1	similarity					1756:1765	100 % 16S rRNA gene sequence similarity	1727:1765	100 % 16S rRNA gene sequence similarity with digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values of 95.3 and 99.4 %, respectively	1727:1885	While analysing the phylogenies of strain HST21T, Streptomyces chryseus DSM 40420T and Streptomyces helvaticus DSM 40431T were found to have 100 % 16S rRNA gene sequence similarity with digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values of 95.3 and 99.4 %, respectively.
31204968	4	76	theme	genome	660:665	arg1	sequences					667:675	the genome sequences	656:675	the genome sequences of strain HST21T and S. albidochromogenes DSM 41800T (35.6 and 88.2 %) and S. flavidovirens DSM 40105T (47.2 and 88.8 %)	656:796	Digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values between the genome sequences of strain HST21T and S. albidochromogenes DSM 41800T (35.6 and 88.2 %) and S. flavidovirens DSM 40105T (47.2 and 88.8 %) were below the thresholds of 70 and 95-96 % for prokaryotic conspecific assignation.
31204968	11	77	theme	later	1932:1936	arg1	synonym					1950:1956	a later heterotypic synonym	1930:1956	a later heterotypic synonym of S. chryseus	1930:1971	Therefore, S. helvaticus is considered as a later heterotypic synonym of S. chryseus and, consequently, we emend the description of S. chryseus.
31204968	11	77	theme	later	1932:1936	arg1	description					2005:2015	the description	2001:2015	the description of S. chryseus	2001:2030	Therefore, S. helvaticus is considered as a later heterotypic synonym of S. chryseus and, consequently, we emend the description of S. chryseus.
31204968	11	77	theme	later	1932:1936	arg1	helvaticus					1902:1911	S. helvaticus	1899:1911	S. helvaticus	1899:1911	Therefore, S. helvaticus is considered as a later heterotypic synonym of S. chryseus and, consequently, we emend the description of S. chryseus.
31204968	4	78	theme	conspecific	858:868	arg1	assignation					870:880	prokaryotic conspecific assignation	846:880	prokaryotic conspecific assignation	846:880	Digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values between the genome sequences of strain HST21T and S. albidochromogenes DSM 41800T (35.6 and 88.2 %) and S. flavidovirens DSM 40105T (47.2 and 88.8 %) were below the thresholds of 70 and 95-96 % for prokaryotic conspecific assignation.
31204968	6	79	theme	whole	1110:1114	arg1	sugars					1121:1126	whole cell sugars	1110:1126	whole cell sugars	1110:1126	Strain HST21T is characterized by the presence of ll-diaminopimelic acid in its peptidoglycan layer; glucose and ribose as whole cell sugars; diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, glycophospholipids, unknown lipids and phospholipids as polar lipids; and anteiso-C15 : 0 (21.6 %) and anteiso-C17 : 0 (20.5 %) as major fatty acids (>15 %).
31204968	10	80	theme	%	1871:1871	arg1	values					1847:1852	digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values	1772:1852	digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values	1772:1852	While analysing the phylogenies of strain HST21T, Streptomyces chryseus DSM 40420T and Streptomyces helvaticus DSM 40431T were found to have 100 % 16S rRNA gene sequence similarity with digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values of 95.3 and 99.4 %, respectively.
31204968	10	81	theme	sequence	1747:1754	arg1	similarity					1756:1765	100 % 16S rRNA gene sequence similarity	1727:1765	100 % 16S rRNA gene sequence similarity with digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values of 95.3 and 99.4 %, respectively	1727:1885	While analysing the phylogenies of strain HST21T, Streptomyces chryseus DSM 40420T and Streptomyces helvaticus DSM 40431T were found to have 100 % 16S rRNA gene sequence similarity with digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values of 95.3 and 99.4 %, respectively.
31204968	11	82	theme	chryseus	1964:1971	arg1	synonym					1950:1956	a later heterotypic synonym	1930:1956	a later heterotypic synonym of S. chryseus	1930:1971	Therefore, S. helvaticus is considered as a later heterotypic synonym of S. chryseus and, consequently, we emend the description of S. chryseus.
31204968	11	82	theme	chryseus	1964:1971	arg1	description					2005:2015	the description	2001:2015	the description of S. chryseus	2001:2030	Therefore, S. helvaticus is considered as a later heterotypic synonym of S. chryseus and, consequently, we emend the description of S. chryseus.
31204968	11	82	theme	chryseus	1964:1971	arg1	helvaticus					1902:1911	S. helvaticus	1899:1911	S. helvaticus	1899:1911	Therefore, S. helvaticus is considered as a later heterotypic synonym of S. chryseus and, consequently, we emend the description of S. chryseus.
31204968	6	83	theme	unknown	1253:1259	arg1	lipids					1261:1266	unknown lipids	1253:1266	unknown lipids	1253:1266	Strain HST21T is characterized by the presence of ll-diaminopimelic acid in its peptidoglycan layer; glucose and ribose as whole cell sugars; diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, glycophospholipids, unknown lipids and phospholipids as polar lipids; and anteiso-C15 : 0 (21.6 %) and anteiso-C17 : 0 (20.5 %) as major fatty acids (>15 %).
31204968	2	84	theme	polyphasic	193:202	arg1	approach					204:211	A polyphasic approach	191:211	A polyphasic approach	191:211	A polyphasic approach was used for evaluating the taxonomic status of strain HST21T isolated from Salar de Huasco in the Atacama Desert.
31204968	1	85	theme	emended	105:111	arg1	description					113:123	emended description	105:123	emended description of Streptomyces chryseus (Krasil'nikov et al. 1965)	105:175	nov., an alkalitolerant species isolated from Chilean Altiplano soil, and emended description of Streptomyces chryseus (Krasil'nikov et al. 1965) Pridham 1970.
31204968	6	86	theme	glycophospholipids	1233:1250	arg1	presence					1025:1032	the presence	1021:1032	the presence of ll-diaminopimelic acid in its peptidoglycan layer; glucose and ribose as whole cell sugars; diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, glycophospholipids, unknown lipids and phospholipids as polar lipids; and anteiso-C15 : 0 (21.6 %) and anteiso-C17 : 0 (20.5 %) as major fatty acids (>15 %)	1021:1388	Strain HST21T is characterized by the presence of ll-diaminopimelic acid in its peptidoglycan layer; glucose and ribose as whole cell sugars; diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, glycophospholipids, unknown lipids and phospholipids as polar lipids; and anteiso-C15 : 0 (21.6 %) and anteiso-C17 : 0 (20.5 %) as major fatty acids (>15 %).
31204968	4	87	theme	S.	698:699	arg1	41800T					723:728	S. albidochromogenes DSM 41800T	698:728	S. albidochromogenes DSM 41800T	698:728	Digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values between the genome sequences of strain HST21T and S. albidochromogenes DSM 41800T (35.6 and 88.2 %) and S. flavidovirens DSM 40105T (47.2 and 88.8 %) were below the thresholds of 70 and 95-96 % for prokaryotic conspecific assignation.
31204968	10	88	theme	DNA-DNA	1780:1786	arg1	dDDH					1803:1806	dDDH	1803:1806	dDDH	1803:1806	While analysing the phylogenies of strain HST21T, Streptomyces chryseus DSM 40420T and Streptomyces helvaticus DSM 40431T were found to have 100 % 16S rRNA gene sequence similarity with digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values of 95.3 and 99.4 %, respectively.
31204968	10	88	theme	DNA-DNA	1780:1786	arg1	hydridization					1788:1800	digital DNA-DNA hydridization	1772:1800	digital DNA-DNA hydridization (dDDH)	1772:1807	While analysing the phylogenies of strain HST21T, Streptomyces chryseus DSM 40420T and Streptomyces helvaticus DSM 40431T were found to have 100 % 16S rRNA gene sequence similarity with digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values of 95.3 and 99.4 %, respectively.
31204968	6	89	theme	phosphatidylinositol	1211:1230	arg1	presence					1025:1032	the presence	1021:1032	the presence of ll-diaminopimelic acid in its peptidoglycan layer; glucose and ribose as whole cell sugars; diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, glycophospholipids, unknown lipids and phospholipids as polar lipids; and anteiso-C15 : 0 (21.6 %) and anteiso-C17 : 0 (20.5 %) as major fatty acids (>15 %)	1021:1388	Strain HST21T is characterized by the presence of ll-diaminopimelic acid in its peptidoglycan layer; glucose and ribose as whole cell sugars; diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, glycophospholipids, unknown lipids and phospholipids as polar lipids; and anteiso-C15 : 0 (21.6 %) and anteiso-C17 : 0 (20.5 %) as major fatty acids (>15 %).
31204968	4	90	theme	DSM	719:721	arg1	41800T					723:728	S. albidochromogenes DSM 41800T	698:728	S. albidochromogenes DSM 41800T	698:728	Digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values between the genome sequences of strain HST21T and S. albidochromogenes DSM 41800T (35.6 and 88.2 %) and S. flavidovirens DSM 40105T (47.2 and 88.8 %) were below the thresholds of 70 and 95-96 % for prokaryotic conspecific assignation.
31204968	6	91	from	lipids	1261:1266	arg1	layer					1081:1085	its peptidoglycan layer	1063:1085	its peptidoglycan layer	1063:1085	Strain HST21T is characterized by the presence of ll-diaminopimelic acid in its peptidoglycan layer; glucose and ribose as whole cell sugars; diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, glycophospholipids, unknown lipids and phospholipids as polar lipids; and anteiso-C15 : 0 (21.6 %) and anteiso-C17 : 0 (20.5 %) as major fatty acids (>15 %).
31204968	6	92	theme	major	1364:1368	arg1	acids					1376:1380	major fatty acids	1364:1380	major fatty acids (>15 %)	1364:1388	Strain HST21T is characterized by the presence of ll-diaminopimelic acid in its peptidoglycan layer; glucose and ribose as whole cell sugars; diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, glycophospholipids, unknown lipids and phospholipids as polar lipids; and anteiso-C15 : 0 (21.6 %) and anteiso-C17 : 0 (20.5 %) as major fatty acids (>15 %).
31204968	6	92	theme	major	1364:1368	arg1	%					1387:1387	>15 %	1383:1387	>15 %	1383:1387	Strain HST21T is characterized by the presence of ll-diaminopimelic acid in its peptidoglycan layer; glucose and ribose as whole cell sugars; diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, glycophospholipids, unknown lipids and phospholipids as polar lipids; and anteiso-C15 : 0 (21.6 %) and anteiso-C17 : 0 (20.5 %) as major fatty acids (>15 %).
31204968	1	93	theme	chryseus	141:148	arg1	species					55:61	an alkalitolerant species	37:61	an alkalitolerant species isolated from Chilean Altiplano soil	37:98	nov., an alkalitolerant species isolated from Chilean Altiplano soil, and emended description of Streptomyces chryseus (Krasil'nikov et al. 1965) Pridham 1970.
31204968	1	93	theme	chryseus	141:148	arg1	description					113:123	emended description	105:123	emended description of Streptomyces chryseus (Krasil'nikov et al. 1965)	105:175	nov., an alkalitolerant species isolated from Chilean Altiplano soil, and emended description of Streptomyces chryseus (Krasil'nikov et al. 1965) Pridham 1970.
31204968	1	93	theme	chryseus	141:148	arg1	nov.					31:34	nov.	31:34	nov.	31:34	nov., an alkalitolerant species isolated from Chilean Altiplano soil, and emended description of Streptomyces chryseus (Krasil'nikov et al. 1965) Pridham 1970.
31204968	6	94	theme	Strain	987:992	arg1	HST21T					994:999	Strain HST21T	987:999	Strain HST21T	987:999	Strain HST21T is characterized by the presence of ll-diaminopimelic acid in its peptidoglycan layer; glucose and ribose as whole cell sugars; diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, glycophospholipids, unknown lipids and phospholipids as polar lipids; and anteiso-C15 : 0 (21.6 %) and anteiso-C17 : 0 (20.5 %) as major fatty acids (>15 %).
31204968	6	95	theme	phosphatidylethanolamine	1185:1208	arg1	presence					1025:1032	the presence	1021:1032	the presence of ll-diaminopimelic acid in its peptidoglycan layer; glucose and ribose as whole cell sugars; diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, glycophospholipids, unknown lipids and phospholipids as polar lipids; and anteiso-C15 : 0 (21.6 %) and anteiso-C17 : 0 (20.5 %) as major fatty acids (>15 %)	1021:1388	Strain HST21T is characterized by the presence of ll-diaminopimelic acid in its peptidoglycan layer; glucose and ribose as whole cell sugars; diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, glycophospholipids, unknown lipids and phospholipids as polar lipids; and anteiso-C15 : 0 (21.6 %) and anteiso-C17 : 0 (20.5 %) as major fatty acids (>15 %).
31204968	7	96	theme	altiplanensis	1500:1512	arg1	sp					1514:1515	the name Streptomyces altiplanensis sp	1478:1515	the name Streptomyces altiplanensis sp	1478:1515	Based on these results, strain HST21T merits recognition as a novel species, for which the name Streptomyces altiplanensis sp.
31204968	4	97	theme	HST21T	687:692	arg1	sequences					667:675	the genome sequences	656:675	the genome sequences of strain HST21T and S. albidochromogenes DSM 41800T (35.6 and 88.2 %) and S. flavidovirens DSM 40105T (47.2 and 88.8 %)	656:796	Digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values between the genome sequences of strain HST21T and S. albidochromogenes DSM 41800T (35.6 and 88.2 %) and S. flavidovirens DSM 40105T (47.2 and 88.8 %) were below the thresholds of 70 and 95-96 % for prokaryotic conspecific assignation.
31204968	11	98	theme	heterotypic	1938:1948	arg1	synonym					1950:1956	a later heterotypic synonym	1930:1956	a later heterotypic synonym of S. chryseus	1930:1971	Therefore, S. helvaticus is considered as a later heterotypic synonym of S. chryseus and, consequently, we emend the description of S. chryseus.
31204968	11	98	theme	heterotypic	1938:1948	arg1	description					2005:2015	the description	2001:2015	the description of S. chryseus	2001:2030	Therefore, S. helvaticus is considered as a later heterotypic synonym of S. chryseus and, consequently, we emend the description of S. chryseus.
31204968	11	98	theme	heterotypic	1938:1948	arg1	helvaticus					1902:1911	S. helvaticus	1899:1911	S. helvaticus	1899:1911	Therefore, S. helvaticus is considered as a later heterotypic synonym of S. chryseus and, consequently, we emend the description of S. chryseus.
31204968	7	99	theme	name	1482:1485	arg1	sp					1514:1515	the name Streptomyces altiplanensis sp	1478:1515	the name Streptomyces altiplanensis sp	1478:1515	Based on these results, strain HST21T merits recognition as a novel species, for which the name Streptomyces altiplanensis sp.
31204968	10	100	contain	have	1722:1725	arg1	40431T					1701:1706	Streptomyces helvaticus DSM 40431T	1673:1706	Streptomyces helvaticus DSM 40431T	1673:1706	While analysing the phylogenies of strain HST21T, Streptomyces chryseus DSM 40420T and Streptomyces helvaticus DSM 40431T were found to have 100 % 16S rRNA gene sequence similarity with digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values of 95.3 and 99.4 %, respectively.
31204968	10	100	contain	have	1722:1725	arg1	40420T					1662:1667	Streptomyces chryseus DSM 40420T	1636:1667	Streptomyces chryseus DSM 40420T	1636:1667	While analysing the phylogenies of strain HST21T, Streptomyces chryseus DSM 40420T and Streptomyces helvaticus DSM 40431T were found to have 100 % 16S rRNA gene sequence similarity with digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values of 95.3 and 99.4 %, respectively.
31204968	10	100	contain	have	1722:1725	arg2	similarity					1756:1765	100 % 16S rRNA gene sequence similarity	1727:1765	100 % 16S rRNA gene sequence similarity with digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values of 95.3 and 99.4 %, respectively	1727:1885	While analysing the phylogenies of strain HST21T, Streptomyces chryseus DSM 40420T and Streptomyces helvaticus DSM 40431T were found to have 100 % 16S rRNA gene sequence similarity with digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values of 95.3 and 99.4 %, respectively.
31204968	5	101	theme	strain	944:949	arg1	HST21T					951:956	strain HST21T	944:956	strain HST21T	944:956	Phenotypic, chemotaxonomic and genetic results distinguished strain HST21T from its closest neighbours.
31204968	10	102	dep	Streptomyces	1673:1684	arg1	helvaticus					1686:1695	helvaticus	1686:1695	helvaticus	1686:1695	While analysing the phylogenies of strain HST21T, Streptomyces chryseus DSM 40420T and Streptomyces helvaticus DSM 40431T were found to have 100 % 16S rRNA gene sequence similarity with digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values of 95.3 and 99.4 %, respectively.
31204968	3	103	theme	strain	412:417	arg1	neighbours					554:563	its nearest neighbours	542:563	its nearest neighbours	542:563	The results of 16S rRNA gene and multilocus sequence phylogenetic analyses assigned strain HST21T to the genus Streptomyceswith Streptomyces albidochromogenes DSM 41800Tand Streptomyces flavidovirens DSM 40150T as its nearest neighbours.
31204968	3	103	theme	strain	412:417	arg1	HST21T					419:424	strain HST21T	412:424	strain HST21T	412:424	The results of 16S rRNA gene and multilocus sequence phylogenetic analyses assigned strain HST21T to the genus Streptomyceswith Streptomyces albidochromogenes DSM 41800Tand Streptomyces flavidovirens DSM 40150T as its nearest neighbours.
31204968	0	104	theme	altiplanensis	13:25	arg1	sp					27:28	Streptomyces altiplanensis sp	0:28	Streptomyces altiplanensis sp.	0:29	Streptomyces altiplanensis sp.
31204968	1	105	theme	Altiplano	85:93	arg1	soil					95:98	Chilean Altiplano soil	77:98	Chilean Altiplano soil	77:98	nov., an alkalitolerant species isolated from Chilean Altiplano soil, and emended description of Streptomyces chryseus (Krasil'nikov et al. 1965) Pridham 1970.
31204968	6	106	theme	ll-diaminopimelic	1037:1053	arg1	acid					1055:1058	ll-diaminopimelic acid	1037:1058	ll-diaminopimelic acid in its peptidoglycan layer	1037:1085	Strain HST21T is characterized by the presence of ll-diaminopimelic acid in its peptidoglycan layer; glucose and ribose as whole cell sugars; diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, glycophospholipids, unknown lipids and phospholipids as polar lipids; and anteiso-C15 : 0 (21.6 %) and anteiso-C17 : 0 (20.5 %) as major fatty acids (>15 %).
31204968	3	107	theme	rRNA	347:350	arg1	gene					352:355	16S rRNA gene	343:355	16S rRNA gene	343:355	The results of 16S rRNA gene and multilocus sequence phylogenetic analyses assigned strain HST21T to the genus Streptomyceswith Streptomyces albidochromogenes DSM 41800Tand Streptomyces flavidovirens DSM 40150T as its nearest neighbours.
31204968	3	108	theme	analyses	394:401	arg1	results					332:338	The results	328:338	The results of 16S rRNA gene and multilocus sequence phylogenetic analyses	328:401	The results of 16S rRNA gene and multilocus sequence phylogenetic analyses assigned strain HST21T to the genus Streptomyceswith Streptomyces albidochromogenes DSM 41800Tand Streptomyces flavidovirens DSM 40150T as its nearest neighbours.
31204968	9	109	theme	107267T=CECT	1566:1577	arg1	strain					1545:1550	The type strain	1536:1550	The type strain	1536:1550	The type strain is HST21T=DSM 107267T=CECT 9647T.
31204968	9	109	theme	107267T=CECT	1566:1577	arg1	9647T					1579:1583	HST21T=DSM 107267T=CECT 9647T	1555:1583	HST21T=DSM 107267T=CECT 9647T	1555:1583	The type strain is HST21T=DSM 107267T=CECT 9647T.
31204968	4	110	theme	DNA-DNA	574:580	arg1	dDDH					597:600	dDDH	597:600	dDDH	597:600	Digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values between the genome sequences of strain HST21T and S. albidochromogenes DSM 41800T (35.6 and 88.2 %) and S. flavidovirens DSM 40105T (47.2 and 88.8 %) were below the thresholds of 70 and 95-96 % for prokaryotic conspecific assignation.
31204968	4	110	theme	DNA-DNA	574:580	arg1	hydridization					582:594	Digital DNA-DNA hydridization	566:594	Digital DNA-DNA hydridization (dDDH)	566:601	Digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values between the genome sequences of strain HST21T and S. albidochromogenes DSM 41800T (35.6 and 88.2 %) and S. flavidovirens DSM 40105T (47.2 and 88.8 %) were below the thresholds of 70 and 95-96 % for prokaryotic conspecific assignation.
31204968	3	111	theme	genus	433:437	arg1	40150T					532:537	the genus Streptomyceswith Streptomyces albidochromogenes DSM 41800Tand Streptomyces flavidovirens DSM 40150T	429:537	the genus Streptomyceswith Streptomyces albidochromogenes DSM 41800Tand Streptomyces flavidovirens DSM 40150T	429:537	The results of 16S rRNA gene and multilocus sequence phylogenetic analyses assigned strain HST21T to the genus Streptomyceswith Streptomyces albidochromogenes DSM 41800Tand Streptomyces flavidovirens DSM 40150T as its nearest neighbours.
31204968	3	112	theme	sequence	372:379	arg1	analyses					394:401	multilocus sequence phylogenetic analyses	361:401	multilocus sequence phylogenetic analyses	361:401	The results of 16S rRNA gene and multilocus sequence phylogenetic analyses assigned strain HST21T to the genus Streptomyceswith Streptomyces albidochromogenes DSM 41800Tand Streptomyces flavidovirens DSM 40150T as its nearest neighbours.
31204968	4	113	theme	47.2	781:784	arg1	40105T					773:778	S. flavidovirens DSM 40105T	752:778	S. flavidovirens DSM 40105T (47.2 and 88.8 %)	752:796	Digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values between the genome sequences of strain HST21T and S. albidochromogenes DSM 41800T (35.6 and 88.2 %) and S. flavidovirens DSM 40105T (47.2 and 88.8 %) were below the thresholds of 70 and 95-96 % for prokaryotic conspecific assignation.
31204968	4	113	theme	47.2	781:784	arg1	%					795:795	47.2 and 88.8 %	781:795	%	795:795	Digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values between the genome sequences of strain HST21T and S. albidochromogenes DSM 41800T (35.6 and 88.2 %) and S. flavidovirens DSM 40105T (47.2 and 88.8 %) were below the thresholds of 70 and 95-96 % for prokaryotic conspecific assignation.
31204968	9	114	theme	type	1540:1543	arg1	strain					1545:1550	The type strain	1536:1550	The type strain	1536:1550	The type strain is HST21T=DSM 107267T=CECT 9647T.
31204968	9	114	theme	type	1540:1543	arg1	9647T					1579:1583	HST21T=DSM 107267T=CECT 9647T	1555:1583	HST21T=DSM 107267T=CECT 9647T	1555:1583	The type strain is HST21T=DSM 107267T=CECT 9647T.
31204968	10	115	theme	identity	1832:1839	arg1	values					1847:1852	digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values	1772:1852	digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values	1772:1852	While analysing the phylogenies of strain HST21T, Streptomyces chryseus DSM 40420T and Streptomyces helvaticus DSM 40431T were found to have 100 % 16S rRNA gene sequence similarity with digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values of 95.3 and 99.4 %, respectively.
31204968	4	116	theme	88.8 	790:794	arg1	40105T					773:778	S. flavidovirens DSM 40105T	752:778	S. flavidovirens DSM 40105T (47.2 and 88.8 %)	752:796	Digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values between the genome sequences of strain HST21T and S. albidochromogenes DSM 41800T (35.6 and 88.2 %) and S. flavidovirens DSM 40105T (47.2 and 88.8 %) were below the thresholds of 70 and 95-96 % for prokaryotic conspecific assignation.
31204968	4	116	theme	88.8 	790:794	arg1	%					795:795	47.2 and 88.8 %	781:795	%	795:795	Digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values between the genome sequences of strain HST21T and S. albidochromogenes DSM 41800T (35.6 and 88.2 %) and S. flavidovirens DSM 40105T (47.2 and 88.8 %) were below the thresholds of 70 and 95-96 % for prokaryotic conspecific assignation.
31204968	1	117	dep	Pridham	177:183	arg1	species					55:61	an alkalitolerant species	37:61	an alkalitolerant species isolated from Chilean Altiplano soil	37:98	nov., an alkalitolerant species isolated from Chilean Altiplano soil, and emended description of Streptomyces chryseus (Krasil'nikov et al. 1965) Pridham 1970.
31204968	1	117	dep	Pridham	177:183	arg1	description					113:123	emended description	105:123	emended description of Streptomyces chryseus (Krasil'nikov et al. 1965)	105:175	nov., an alkalitolerant species isolated from Chilean Altiplano soil, and emended description of Streptomyces chryseus (Krasil'nikov et al. 1965) Pridham 1970.
31204968	1	117	dep	Pridham	177:183	arg1	nov.					31:34	nov.	31:34	nov.	31:34	nov., an alkalitolerant species isolated from Chilean Altiplano soil, and emended description of Streptomyces chryseus (Krasil'nikov et al. 1965) Pridham 1970.
31204968	4	118	theme	average	607:613	arg1	ANI					636:638	ANI	636:638	ANI	636:638	Digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values between the genome sequences of strain HST21T and S. albidochromogenes DSM 41800T (35.6 and 88.2 %) and S. flavidovirens DSM 40105T (47.2 and 88.8 %) were below the thresholds of 70 and 95-96 % for prokaryotic conspecific assignation.
31204968	4	118	theme	average	607:613	arg1	identity					626:633	average nucleotide identity	607:633	average nucleotide identity (ANI)	607:639	Digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values between the genome sequences of strain HST21T and S. albidochromogenes DSM 41800T (35.6 and 88.2 %) and S. flavidovirens DSM 40105T (47.2 and 88.8 %) were below the thresholds of 70 and 95-96 % for prokaryotic conspecific assignation.
31204968	10	119	theme	average	1813:1819	arg1	ANI					1842:1844	ANI	1842:1844	ANI	1842:1844	While analysing the phylogenies of strain HST21T, Streptomyces chryseus DSM 40420T and Streptomyces helvaticus DSM 40431T were found to have 100 % 16S rRNA gene sequence similarity with digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values of 95.3 and 99.4 %, respectively.
31204968	10	119	theme	average	1813:1819	arg1	identity					1832:1839	average nucleotide identity	1813:1839	average nucleotide identity (ANI)	1813:1845	While analysing the phylogenies of strain HST21T, Streptomyces chryseus DSM 40420T and Streptomyces helvaticus DSM 40431T were found to have 100 % 16S rRNA gene sequence similarity with digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values of 95.3 and 99.4 %, respectively.
31204968	4	120	theme	flavidovirens	755:767	arg1	40105T					773:778	S. flavidovirens DSM 40105T	752:778	S. flavidovirens DSM 40105T (47.2 and 88.8 %)	752:796	Digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values between the genome sequences of strain HST21T and S. albidochromogenes DSM 41800T (35.6 and 88.2 %) and S. flavidovirens DSM 40105T (47.2 and 88.8 %) were below the thresholds of 70 and 95-96 % for prokaryotic conspecific assignation.
31204968	4	120	theme	flavidovirens	755:767	arg1	%					795:795	47.2 and 88.8 %	781:795	%	795:795	Digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values between the genome sequences of strain HST21T and S. albidochromogenes DSM 41800T (35.6 and 88.2 %) and S. flavidovirens DSM 40105T (47.2 and 88.8 %) were below the thresholds of 70 and 95-96 % for prokaryotic conspecific assignation.
31204968	10	121	theme	Streptomyces	1673:1684	arg1	40431T					1701:1706	Streptomyces helvaticus DSM 40431T	1673:1706	Streptomyces helvaticus DSM 40431T	1673:1706	While analysing the phylogenies of strain HST21T, Streptomyces chryseus DSM 40420T and Streptomyces helvaticus DSM 40431T were found to have 100 % 16S rRNA gene sequence similarity with digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values of 95.3 and 99.4 %, respectively.
31204968	5	122	theme	closest	967:973	arg1	neighbours					975:984	its closest neighbours	963:984	its closest neighbours	963:984	Phenotypic, chemotaxonomic and genetic results distinguished strain HST21T from its closest neighbours.
31204968	4	123	theme	identity	626:633	arg1	values					641:646	Digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values	566:646	Digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values between the genome sequences of strain HST21T and S. albidochromogenes DSM 41800T (35.6 and 88.2 %) and S. flavidovirens DSM 40105T (47.2 and 88.8 %)	566:796	Digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values between the genome sequences of strain HST21T and S. albidochromogenes DSM 41800T (35.6 and 88.2 %) and S. flavidovirens DSM 40105T (47.2 and 88.8 %) were below the thresholds of 70 and 95-96 % for prokaryotic conspecific assignation.
31204968	4	124	theme	40105T	773:778	arg1	sequences					667:675	the genome sequences	656:675	the genome sequences of strain HST21T and S. albidochromogenes DSM 41800T (35.6 and 88.2 %) and S. flavidovirens DSM 40105T (47.2 and 88.8 %)	656:796	Digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values between the genome sequences of strain HST21T and S. albidochromogenes DSM 41800T (35.6 and 88.2 %) and S. flavidovirens DSM 40105T (47.2 and 88.8 %) were below the thresholds of 70 and 95-96 % for prokaryotic conspecific assignation.
31204968	3	125	theme	DSM	528:530	arg1	40150T					532:537	the genus Streptomyceswith Streptomyces albidochromogenes DSM 41800Tand Streptomyces flavidovirens DSM 40150T	429:537	the genus Streptomyceswith Streptomyces albidochromogenes DSM 41800Tand Streptomyces flavidovirens DSM 40150T	429:537	The results of 16S rRNA gene and multilocus sequence phylogenetic analyses assigned strain HST21T to the genus Streptomyceswith Streptomyces albidochromogenes DSM 41800Tand Streptomyces flavidovirens DSM 40150T as its nearest neighbours.
29912150	0	0	theme	myrtifolia	81:90	arg1	Chinotto					64:71	Chinotto	64:71	Chinotto (Citrus myrtifolia Raf.)	64:96	In Vitro Anti-Inflammatory and Radical Scavenging Properties of Chinotto (Citrus myrtifolia Raf.)
29912150	0	0	theme	myrtifolia	81:90	arg1	Raf					92:94	Citrus myrtifolia Raf.	74:95	Citrus myrtifolia Raf.	74:95	In Vitro Anti-Inflammatory and Radical Scavenging Properties of Chinotto (Citrus myrtifolia Raf.)
29912150	6	1	theme	oxide	842:846	arg1	genes					789:793	inflammatory genes	776:793	inflammatory genes	776:793	Essential oil from half ripe chinotto (CEO2) reduced the production of nitric oxide (NO) and the expression of inflammatory genes, cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), cytokines, including interleukin-1&beta; (IL-1&beta;) and interleukin-6 (IL-6), and chemokine monocyte chemotactic protein-1 (MCP-1) by lipopolysaccharide (LPS)-stimulated RAW264,7 macrophages.
29912150	6	1	theme	oxide	842:846	arg1	interleukin-1&beta					886:903	interleukin-1&beta;	886:904	interleukin-1&beta; (IL-1&beta;)	886:917	Essential oil from half ripe chinotto (CEO2) reduced the production of nitric oxide (NO) and the expression of inflammatory genes, cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), cytokines, including interleukin-1&beta; (IL-1&beta;) and interleukin-6 (IL-6), and chemokine monocyte chemotactic protein-1 (MCP-1) by lipopolysaccharide (LPS)-stimulated RAW264,7 macrophages.
29912150	6	1	theme	oxide	842:846	arg1	interleukin-6					923:935	interleukin-6	923:935	interleukin-6 (IL-6)	923:942	Essential oil from half ripe chinotto (CEO2) reduced the production of nitric oxide (NO) and the expression of inflammatory genes, cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), cytokines, including interleukin-1&beta; (IL-1&beta;) and interleukin-6 (IL-6), and chemokine monocyte chemotactic protein-1 (MCP-1) by lipopolysaccharide (LPS)-stimulated RAW264,7 macrophages.
29912150	6	1	theme	oxide	842:846	arg1	iNOS					858:861	iNOS	858:861	iNOS	858:861	Essential oil from half ripe chinotto (CEO2) reduced the production of nitric oxide (NO) and the expression of inflammatory genes, cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), cytokines, including interleukin-1&beta; (IL-1&beta;) and interleukin-6 (IL-6), and chemokine monocyte chemotactic protein-1 (MCP-1) by lipopolysaccharide (LPS)-stimulated RAW264,7 macrophages.
29912150	6	1	theme	oxide	842:846	arg1	synthase					848:855	inducible nitric oxide synthase	825:855	inducible nitric oxide synthase (iNOS)	825:862	Essential oil from half ripe chinotto (CEO2) reduced the production of nitric oxide (NO) and the expression of inflammatory genes, cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), cytokines, including interleukin-1&beta; (IL-1&beta;) and interleukin-6 (IL-6), and chemokine monocyte chemotactic protein-1 (MCP-1) by lipopolysaccharide (LPS)-stimulated RAW264,7 macrophages.
29912150	5	2	theme	anti-inflammatory	482:498	arg1	properties					523:532	potential in vitro anti-inflammatory and radical scavenging properties	463:532	potential in vitro anti-inflammatory and radical scavenging properties of chinotto essential oils (CEOs)	463:566	The present study aimed to investigate potential in vitro anti-inflammatory and radical scavenging properties of chinotto essential oils (CEOs) and to establish to what extent their composition and bioactivities are dependent on maturation.
29912150	6	3	from	chinotto	694:701	arg1	oil					675:677	Essential oil	665:677	Essential oil from half ripe chinotto (CEO2)	665:708	Essential oil from half ripe chinotto (CEO2) reduced the production of nitric oxide (NO) and the expression of inflammatory genes, cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), cytokines, including interleukin-1&beta; (IL-1&beta;) and interleukin-6 (IL-6), and chemokine monocyte chemotactic protein-1 (MCP-1) by lipopolysaccharide (LPS)-stimulated RAW264,7 macrophages.
29912150	0	4	theme	Citrus	74:79	arg1	Chinotto					64:71	Chinotto	64:71	Chinotto (Citrus myrtifolia Raf.)	64:96	In Vitro Anti-Inflammatory and Radical Scavenging Properties of Chinotto (Citrus myrtifolia Raf.)
29912150	0	4	theme	Citrus	74:79	arg1	Raf					92:94	Citrus myrtifolia Raf.	74:95	Citrus myrtifolia Raf.	74:95	In Vitro Anti-Inflammatory and Radical Scavenging Properties of Chinotto (Citrus myrtifolia Raf.)
29912150	6	5	theme	nitric	835:840	arg1	genes					789:793	inflammatory genes	776:793	inflammatory genes	776:793	Essential oil from half ripe chinotto (CEO2) reduced the production of nitric oxide (NO) and the expression of inflammatory genes, cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), cytokines, including interleukin-1&beta; (IL-1&beta;) and interleukin-6 (IL-6), and chemokine monocyte chemotactic protein-1 (MCP-1) by lipopolysaccharide (LPS)-stimulated RAW264,7 macrophages.
29912150	6	5	theme	nitric	835:840	arg1	interleukin-1&beta					886:903	interleukin-1&beta;	886:904	interleukin-1&beta; (IL-1&beta;)	886:917	Essential oil from half ripe chinotto (CEO2) reduced the production of nitric oxide (NO) and the expression of inflammatory genes, cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), cytokines, including interleukin-1&beta; (IL-1&beta;) and interleukin-6 (IL-6), and chemokine monocyte chemotactic protein-1 (MCP-1) by lipopolysaccharide (LPS)-stimulated RAW264,7 macrophages.
29912150	6	5	theme	nitric	835:840	arg1	interleukin-6					923:935	interleukin-6	923:935	interleukin-6 (IL-6)	923:942	Essential oil from half ripe chinotto (CEO2) reduced the production of nitric oxide (NO) and the expression of inflammatory genes, cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), cytokines, including interleukin-1&beta; (IL-1&beta;) and interleukin-6 (IL-6), and chemokine monocyte chemotactic protein-1 (MCP-1) by lipopolysaccharide (LPS)-stimulated RAW264,7 macrophages.
29912150	6	5	theme	nitric	835:840	arg1	iNOS					858:861	iNOS	858:861	iNOS	858:861	Essential oil from half ripe chinotto (CEO2) reduced the production of nitric oxide (NO) and the expression of inflammatory genes, cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), cytokines, including interleukin-1&beta; (IL-1&beta;) and interleukin-6 (IL-6), and chemokine monocyte chemotactic protein-1 (MCP-1) by lipopolysaccharide (LPS)-stimulated RAW264,7 macrophages.
29912150	6	5	theme	nitric	835:840	arg1	synthase					848:855	inducible nitric oxide synthase	825:855	inducible nitric oxide synthase (iNOS)	825:862	Essential oil from half ripe chinotto (CEO2) reduced the production of nitric oxide (NO) and the expression of inflammatory genes, cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), cytokines, including interleukin-1&beta; (IL-1&beta;) and interleukin-6 (IL-6), and chemokine monocyte chemotactic protein-1 (MCP-1) by lipopolysaccharide (LPS)-stimulated RAW264,7 macrophages.
29912150	6	6	theme	inflammatory	776:787	arg1	cytokines					865:873	cytokines	865:873	cytokines	865:873	Essential oil from half ripe chinotto (CEO2) reduced the production of nitric oxide (NO) and the expression of inflammatory genes, cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), cytokines, including interleukin-1&beta; (IL-1&beta;) and interleukin-6 (IL-6), and chemokine monocyte chemotactic protein-1 (MCP-1) by lipopolysaccharide (LPS)-stimulated RAW264,7 macrophages.
29912150	6	6	theme	inflammatory	776:787	arg1	synthase					848:855	inducible nitric oxide synthase	825:855	inducible nitric oxide synthase (iNOS)	825:862	Essential oil from half ripe chinotto (CEO2) reduced the production of nitric oxide (NO) and the expression of inflammatory genes, cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), cytokines, including interleukin-1&beta; (IL-1&beta;) and interleukin-6 (IL-6), and chemokine monocyte chemotactic protein-1 (MCP-1) by lipopolysaccharide (LPS)-stimulated RAW264,7 macrophages.
29912150	6	6	theme	inflammatory	776:787	arg1	interleukin-1&beta					886:903	interleukin-1&beta;	886:904	interleukin-1&beta; (IL-1&beta;)	886:917	Essential oil from half ripe chinotto (CEO2) reduced the production of nitric oxide (NO) and the expression of inflammatory genes, cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), cytokines, including interleukin-1&beta; (IL-1&beta;) and interleukin-6 (IL-6), and chemokine monocyte chemotactic protein-1 (MCP-1) by lipopolysaccharide (LPS)-stimulated RAW264,7 macrophages.
29912150	6	6	theme	inflammatory	776:787	arg1	interleukin-6					923:935	interleukin-6	923:935	interleukin-6 (IL-6)	923:942	Essential oil from half ripe chinotto (CEO2) reduced the production of nitric oxide (NO) and the expression of inflammatory genes, cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), cytokines, including interleukin-1&beta; (IL-1&beta;) and interleukin-6 (IL-6), and chemokine monocyte chemotactic protein-1 (MCP-1) by lipopolysaccharide (LPS)-stimulated RAW264,7 macrophages.
29912150	6	6	theme	inflammatory	776:787	arg1	genes					789:793	inflammatory genes	776:793	inflammatory genes	776:793	Essential oil from half ripe chinotto (CEO2) reduced the production of nitric oxide (NO) and the expression of inflammatory genes, cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), cytokines, including interleukin-1&beta; (IL-1&beta;) and interleukin-6 (IL-6), and chemokine monocyte chemotactic protein-1 (MCP-1) by lipopolysaccharide (LPS)-stimulated RAW264,7 macrophages.
29912150	6	6	theme	inflammatory	776:787	arg1	cyclooxygenase-2					796:811	cyclooxygenase-2	796:811	cyclooxygenase-2 (COX-2)	796:819	Essential oil from half ripe chinotto (CEO2) reduced the production of nitric oxide (NO) and the expression of inflammatory genes, cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), cytokines, including interleukin-1&beta; (IL-1&beta;) and interleukin-6 (IL-6), and chemokine monocyte chemotactic protein-1 (MCP-1) by lipopolysaccharide (LPS)-stimulated RAW264,7 macrophages.
29912150	5	7	theme	chinotto	537:544	arg1	CEOs					562:565	CEOs	562:565	CEOs	562:565	The present study aimed to investigate potential in vitro anti-inflammatory and radical scavenging properties of chinotto essential oils (CEOs) and to establish to what extent their composition and bioactivities are dependent on maturation.
29912150	5	7	theme	chinotto	537:544	arg1	oils					556:559	chinotto essential oils	537:559	chinotto essential oils (CEOs)	537:566	The present study aimed to investigate potential in vitro anti-inflammatory and radical scavenging properties of chinotto essential oils (CEOs) and to establish to what extent their composition and bioactivities are dependent on maturation.
29912150	5	8	dep	in	473:474	arg1	vitro					476:480	vitro	476:480	vitro	476:480	The present study aimed to investigate potential in vitro anti-inflammatory and radical scavenging properties of chinotto essential oils (CEOs) and to establish to what extent their composition and bioactivities are dependent on maturation.
29912150	6	9	theme	-stimulated	1025:1035	arg1	macrophages					1046:1056	lipopolysaccharide (LPS)-stimulated RAW264,7 macrophages	1001:1056	lipopolysaccharide (LPS)-stimulated RAW264,7 macrophages	1001:1056	Essential oil from half ripe chinotto (CEO2) reduced the production of nitric oxide (NO) and the expression of inflammatory genes, cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), cytokines, including interleukin-1&beta; (IL-1&beta;) and interleukin-6 (IL-6), and chemokine monocyte chemotactic protein-1 (MCP-1) by lipopolysaccharide (LPS)-stimulated RAW264,7 macrophages.
29912150	7	10	from	components	1144:1153	arg1	CEO2					1158:1161	CEO2	1158:1161	CEO2	1158:1161	Limonene, linalool, linalyl acetate, and &gamma;-terpinene were found to be the main components in CEO2.
29912150	9	11	theme	valuable	1462:1469	arg1	part					1471:1474	a valuable part	1460:1474	a valuable part of this fruit	1460:1488	These findings show that chinotto essential oil represents a valuable part of this fruit and warrants further in vivo studies to validate its anti-inflammatory potential.
29912150	8	12	theme	scavenging	1199:1208	arg1	activity					1210:1217	high radical scavenging activity	1186:1217	high radical scavenging activity measured as Trolox equivalents (TE) against both 2,2&prime;-diphenyl-1-picrylhydrazyl (DPPH) and 2,2&prime;-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS)	1186:1398	Moreover, CEO2 showed high radical scavenging activity measured as Trolox equivalents (TE) against both 2,2&prime;-diphenyl-1-picrylhydrazyl (DPPH) and 2,2&prime;-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS).
29912150	7	13	theme	linalyl	1079:1085	arg1	components					1144:1153	the main components	1135:1153	the main components in CEO2	1135:1161	Limonene, linalool, linalyl acetate, and &gamma;-terpinene were found to be the main components in CEO2.
29912150	7	13	theme	linalyl	1079:1085	arg1	-terpinene					1107:1116	&gamma;-terpinene	1100:1116	&gamma;-terpinene	1100:1116	Limonene, linalool, linalyl acetate, and &gamma;-terpinene were found to be the main components in CEO2.
29912150	7	13	theme	linalyl	1079:1085	arg1	linalool					1069:1076	linalool	1069:1076	linalool	1069:1076	Limonene, linalool, linalyl acetate, and &gamma;-terpinene were found to be the main components in CEO2.
29912150	7	13	theme	linalyl	1079:1085	arg1	acetate					1087:1093	linalyl acetate	1079:1093	linalyl acetate	1079:1093	Limonene, linalool, linalyl acetate, and &gamma;-terpinene were found to be the main components in CEO2.
29912150	7	13	theme	linalyl	1079:1085	arg1	Limonene					1059:1066	Limonene	1059:1066	Limonene	1059:1066	Limonene, linalool, linalyl acetate, and &gamma;-terpinene were found to be the main components in CEO2.
29912150	3	14	theme	wide-spread	215:225	arg1	use					227:229	a wide-spread use	213:229	a wide-spread use in confectionary and drinks	213:257	is a widely diffused plant native from China and its fruits have a wide-spread use in confectionary and drinks.
29912150	6	15	theme	oxide	743:747	arg1	expression					762:771	the expression	758:771	the expression of inflammatory genes, cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), cytokines, including interleukin-1&beta; (IL-1&beta;) and interleukin-6 (IL-6)	758:942	Essential oil from half ripe chinotto (CEO2) reduced the production of nitric oxide (NO) and the expression of inflammatory genes, cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), cytokines, including interleukin-1&beta; (IL-1&beta;) and interleukin-6 (IL-6), and chemokine monocyte chemotactic protein-1 (MCP-1) by lipopolysaccharide (LPS)-stimulated RAW264,7 macrophages.
29912150	6	15	theme	oxide	743:747	arg1	MCP-1					991:995	MCP-1	991:995	MCP-1	991:995	Essential oil from half ripe chinotto (CEO2) reduced the production of nitric oxide (NO) and the expression of inflammatory genes, cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), cytokines, including interleukin-1&beta; (IL-1&beta;) and interleukin-6 (IL-6), and chemokine monocyte chemotactic protein-1 (MCP-1) by lipopolysaccharide (LPS)-stimulated RAW264,7 macrophages.
29912150	6	15	theme	oxide	743:747	arg1	production					722:731	the production	718:731	the production of nitric oxide (NO)	718:752	Essential oil from half ripe chinotto (CEO2) reduced the production of nitric oxide (NO) and the expression of inflammatory genes, cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), cytokines, including interleukin-1&beta; (IL-1&beta;) and interleukin-6 (IL-6), and chemokine monocyte chemotactic protein-1 (MCP-1) by lipopolysaccharide (LPS)-stimulated RAW264,7 macrophages.
29912150	6	15	theme	oxide	743:747	arg1	protein-1					980:988	chemokine monocyte chemotactic protein-1	949:988	chemokine monocyte chemotactic protein-1 (MCP-1)	949:996	Essential oil from half ripe chinotto (CEO2) reduced the production of nitric oxide (NO) and the expression of inflammatory genes, cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), cytokines, including interleukin-1&beta; (IL-1&beta;) and interleukin-6 (IL-6), and chemokine monocyte chemotactic protein-1 (MCP-1) by lipopolysaccharide (LPS)-stimulated RAW264,7 macrophages.
29912150	8	16	theme	Trolox	1231:1236	arg1	TE					1251:1252	TE	1251:1252	TE	1251:1252	Moreover, CEO2 showed high radical scavenging activity measured as Trolox equivalents (TE) against both 2,2&prime;-diphenyl-1-picrylhydrazyl (DPPH) and 2,2&prime;-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS).
29912150	8	16	theme	Trolox	1231:1236	arg1	equivalents					1238:1248	Trolox equivalents	1231:1248	Trolox equivalents (TE) against both 2,2&prime;-diphenyl-1-picrylhydrazyl (DPPH) and 2,2&prime;-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS)	1231:1398	Moreover, CEO2 showed high radical scavenging activity measured as Trolox equivalents (TE) against both 2,2&prime;-diphenyl-1-picrylhydrazyl (DPPH) and 2,2&prime;-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS).
29912150	6	17	theme	Essential	665:673	arg1	oil					675:677	Essential oil	665:677	Essential oil from half ripe chinotto (CEO2)	665:708	Essential oil from half ripe chinotto (CEO2) reduced the production of nitric oxide (NO) and the expression of inflammatory genes, cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), cytokines, including interleukin-1&beta; (IL-1&beta;) and interleukin-6 (IL-6), and chemokine monocyte chemotactic protein-1 (MCP-1) by lipopolysaccharide (LPS)-stimulated RAW264,7 macrophages.
29912150	2	18	theme	myrtifolia	131:140	arg1	Chinotto					114:121	Chinotto	114:121	Chinotto (Citrus myrtifolia Raf.)	114:146	Chinotto (Citrus myrtifolia Raf.)
29912150	2	18	theme	myrtifolia	131:140	arg1	Raf					142:144	Citrus myrtifolia Raf.	124:145	Citrus myrtifolia Raf.	124:145	Chinotto (Citrus myrtifolia Raf.)
29912150	9	19	theme	anti-inflammatory	1543:1559	arg1	potential					1561:1569	its anti-inflammatory potential	1539:1569	its anti-inflammatory potential	1539:1569	These findings show that chinotto essential oil represents a valuable part of this fruit and warrants further in vivo studies to validate its anti-inflammatory potential.
29912150	3	20	theme	native	175:180	arg1	plant					169:173	a widely diffused plant	151:173	a widely diffused plant native from China	151:191	is a widely diffused plant native from China and its fruits have a wide-spread use in confectionary and drinks.
29912150	9	21	theme	essential	1435:1443	arg1	oil					1445:1447	chinotto essential oil	1426:1447	chinotto essential oil	1426:1447	These findings show that chinotto essential oil represents a valuable part of this fruit and warrants further in vivo studies to validate its anti-inflammatory potential.
29912150	6	22	theme	chemotactic	968:978	arg1	MCP-1					991:995	MCP-1	991:995	MCP-1	991:995	Essential oil from half ripe chinotto (CEO2) reduced the production of nitric oxide (NO) and the expression of inflammatory genes, cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), cytokines, including interleukin-1&beta; (IL-1&beta;) and interleukin-6 (IL-6), and chemokine monocyte chemotactic protein-1 (MCP-1) by lipopolysaccharide (LPS)-stimulated RAW264,7 macrophages.
29912150	6	22	theme	chemotactic	968:978	arg1	protein-1					980:988	chemokine monocyte chemotactic protein-1	949:988	chemokine monocyte chemotactic protein-1 (MCP-1)	949:996	Essential oil from half ripe chinotto (CEO2) reduced the production of nitric oxide (NO) and the expression of inflammatory genes, cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), cytokines, including interleukin-1&beta; (IL-1&beta;) and interleukin-6 (IL-6), and chemokine monocyte chemotactic protein-1 (MCP-1) by lipopolysaccharide (LPS)-stimulated RAW264,7 macrophages.
29912150	2	23	theme	Citrus	124:129	arg1	Chinotto					114:121	Chinotto	114:121	Chinotto (Citrus myrtifolia Raf.)	114:146	Chinotto (Citrus myrtifolia Raf.)
29912150	2	23	theme	Citrus	124:129	arg1	Raf					142:144	Citrus myrtifolia Raf.	124:145	Citrus myrtifolia Raf.	124:145	Chinotto (Citrus myrtifolia Raf.)
29912150	4	24	theme	bioactive	318:326	arg1	properties					328:337	its bioactive properties	314:337	its bioactive properties	314:337	Remarkably, only little has been reported thus far on its bioactive properties, in contrast to those of the taxonomically related bergamot (Citrus bergamia Risso).
29912150	6	25	theme	monocyte	959:966	arg1	MCP-1					991:995	MCP-1	991:995	MCP-1	991:995	Essential oil from half ripe chinotto (CEO2) reduced the production of nitric oxide (NO) and the expression of inflammatory genes, cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), cytokines, including interleukin-1&beta; (IL-1&beta;) and interleukin-6 (IL-6), and chemokine monocyte chemotactic protein-1 (MCP-1) by lipopolysaccharide (LPS)-stimulated RAW264,7 macrophages.
29912150	6	25	theme	monocyte	959:966	arg1	protein-1					980:988	chemokine monocyte chemotactic protein-1	949:988	chemokine monocyte chemotactic protein-1 (MCP-1)	949:996	Essential oil from half ripe chinotto (CEO2) reduced the production of nitric oxide (NO) and the expression of inflammatory genes, cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), cytokines, including interleukin-1&beta; (IL-1&beta;) and interleukin-6 (IL-6), and chemokine monocyte chemotactic protein-1 (MCP-1) by lipopolysaccharide (LPS)-stimulated RAW264,7 macrophages.
29912150	0	26	theme	In	0:1	arg1	Properties					50:59	In Vitro Anti-Inflammatory and Radical Scavenging Properties	0:59	In Vitro Anti-Inflammatory and Radical Scavenging Properties of Chinotto (Citrus myrtifolia Raf.)	0:96	In Vitro Anti-Inflammatory and Radical Scavenging Properties of Chinotto (Citrus myrtifolia Raf.)
29912150	8	27	theme	acid	1371:1374	arg1	ABTS					1394:1397	ABTS	1394:1397	ABTS	1394:1397	Moreover, CEO2 showed high radical scavenging activity measured as Trolox equivalents (TE) against both 2,2&prime;-diphenyl-1-picrylhydrazyl (DPPH) and 2,2&prime;-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS).
29912150	8	27	theme	acid	1371:1374	arg1	salt					1388:1391	-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt	1326:1391	both 2,2&prime;-diphenyl-1-picrylhydrazyl (DPPH) and 2,2&prime;-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS)	1263:1398	Moreover, CEO2 showed high radical scavenging activity measured as Trolox equivalents (TE) against both 2,2&prime;-diphenyl-1-picrylhydrazyl (DPPH) and 2,2&prime;-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS).
29912150	3	28	theme	diffused	160:167	arg1	plant					169:173	a widely diffused plant	151:173	a widely diffused plant native from China	151:191	is a widely diffused plant native from China and its fruits have a wide-spread use in confectionary and drinks.
29912150	8	29	theme	3-ethylbenzothiazoline-6-sulfonic	1337:1369	arg1	ABTS					1394:1397	ABTS	1394:1397	ABTS	1394:1397	Moreover, CEO2 showed high radical scavenging activity measured as Trolox equivalents (TE) against both 2,2&prime;-diphenyl-1-picrylhydrazyl (DPPH) and 2,2&prime;-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS).
29912150	8	29	theme	3-ethylbenzothiazoline-6-sulfonic	1337:1369	arg1	salt					1388:1391	-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt	1326:1391	both 2,2&prime;-diphenyl-1-picrylhydrazyl (DPPH) and 2,2&prime;-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS)	1263:1398	Moreover, CEO2 showed high radical scavenging activity measured as Trolox equivalents (TE) against both 2,2&prime;-diphenyl-1-picrylhydrazyl (DPPH) and 2,2&prime;-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS).
29912150	6	30	theme	chemokine	949:957	arg1	MCP-1					991:995	MCP-1	991:995	MCP-1	991:995	Essential oil from half ripe chinotto (CEO2) reduced the production of nitric oxide (NO) and the expression of inflammatory genes, cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), cytokines, including interleukin-1&beta; (IL-1&beta;) and interleukin-6 (IL-6), and chemokine monocyte chemotactic protein-1 (MCP-1) by lipopolysaccharide (LPS)-stimulated RAW264,7 macrophages.
29912150	6	30	theme	chemokine	949:957	arg1	protein-1					980:988	chemokine monocyte chemotactic protein-1	949:988	chemokine monocyte chemotactic protein-1 (MCP-1)	949:996	Essential oil from half ripe chinotto (CEO2) reduced the production of nitric oxide (NO) and the expression of inflammatory genes, cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), cytokines, including interleukin-1&beta; (IL-1&beta;) and interleukin-6 (IL-6), and chemokine monocyte chemotactic protein-1 (MCP-1) by lipopolysaccharide (LPS)-stimulated RAW264,7 macrophages.
29912150	5	31	theme	essential	546:554	arg1	CEOs					562:565	CEOs	562:565	CEOs	562:565	The present study aimed to investigate potential in vitro anti-inflammatory and radical scavenging properties of chinotto essential oils (CEOs) and to establish to what extent their composition and bioactivities are dependent on maturation.
29912150	5	31	theme	essential	546:554	arg1	oils					556:559	chinotto essential oils	537:559	chinotto essential oils (CEOs)	537:566	The present study aimed to investigate potential in vitro anti-inflammatory and radical scavenging properties of chinotto essential oils (CEOs) and to establish to what extent their composition and bioactivities are dependent on maturation.
29912150	5	32	theme	oils	556:559	arg1	properties					523:532	potential in vitro anti-inflammatory and radical scavenging properties	463:532	potential in vitro anti-inflammatory and radical scavenging properties of chinotto essential oils (CEOs)	463:566	The present study aimed to investigate potential in vitro anti-inflammatory and radical scavenging properties of chinotto essential oils (CEOs) and to establish to what extent their composition and bioactivities are dependent on maturation.
29912150	6	33	theme	RAW264,7	1037:1044	arg1	macrophages					1046:1056	lipopolysaccharide (LPS)-stimulated RAW264,7 macrophages	1001:1056	lipopolysaccharide (LPS)-stimulated RAW264,7 macrophages	1001:1056	Essential oil from half ripe chinotto (CEO2) reduced the production of nitric oxide (NO) and the expression of inflammatory genes, cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), cytokines, including interleukin-1&beta; (IL-1&beta;) and interleukin-6 (IL-6), and chemokine monocyte chemotactic protein-1 (MCP-1) by lipopolysaccharide (LPS)-stimulated RAW264,7 macrophages.
29912150	0	34	theme	Anti-Inflammatory	9:25	arg1	Properties					50:59	In Vitro Anti-Inflammatory and Radical Scavenging Properties	0:59	In Vitro Anti-Inflammatory and Radical Scavenging Properties of Chinotto (Citrus myrtifolia Raf.)	0:96	In Vitro Anti-Inflammatory and Radical Scavenging Properties of Chinotto (Citrus myrtifolia Raf.)
29912150	9	35	theme	chinotto	1426:1433	arg1	oil					1445:1447	chinotto essential oil	1426:1447	chinotto essential oil	1426:1447	These findings show that chinotto essential oil represents a valuable part of this fruit and warrants further in vivo studies to validate its anti-inflammatory potential.
29912150	4	36	theme	Citrus	400:405	arg1	bergamot					390:397	the taxonomically related bergamot	364:397	the taxonomically related bergamot (Citrus bergamia Risso)	364:421	Remarkably, only little has been reported thus far on its bioactive properties, in contrast to those of the taxonomically related bergamot (Citrus bergamia Risso).
29912150	4	36	theme	Citrus	400:405	arg1	Risso					416:420	Citrus bergamia Risso	400:420	Citrus bergamia Risso	400:420	Remarkably, only little has been reported thus far on its bioactive properties, in contrast to those of the taxonomically related bergamot (Citrus bergamia Risso).
29912150	1	37	theme	Essential	98:106	arg1	Oils					108:111	Essential Oils	98:111	Essential Oils.	98:112	Essential Oils.
29912150	5	38	dep	potential	463:471	arg1	in					473:474	in	473:474	in	473:474	The present study aimed to investigate potential in vitro anti-inflammatory and radical scavenging properties of chinotto essential oils (CEOs) and to establish to what extent their composition and bioactivities are dependent on maturation.
29912150	9	39	dep	in	1511:1512	arg1	vivo					1514:1517	vivo	1514:1517	vivo	1514:1517	These findings show that chinotto essential oil represents a valuable part of this fruit and warrants further in vivo studies to validate its anti-inflammatory potential.
29912150	0	40	theme	Scavenging	39:48	arg1	Properties					50:59	In Vitro Anti-Inflammatory and Radical Scavenging Properties	0:59	In Vitro Anti-Inflammatory and Radical Scavenging Properties of Chinotto (Citrus myrtifolia Raf.)	0:96	In Vitro Anti-Inflammatory and Radical Scavenging Properties of Chinotto (Citrus myrtifolia Raf.)
29912150	3	41	from	use	227:229	arg1	drinks					252:257	drinks	252:257	drinks	252:257	is a widely diffused plant native from China and its fruits have a wide-spread use in confectionary and drinks.
29912150	3	41	from	use	227:229	arg1	confectionary					234:246	confectionary	234:246	confectionary	234:246	is a widely diffused plant native from China and its fruits have a wide-spread use in confectionary and drinks.
29912150	5	42	theme	present	428:434	arg1	study					436:440	The present study	424:440	The present study	424:440	The present study aimed to investigate potential in vitro anti-inflammatory and radical scavenging properties of chinotto essential oils (CEOs) and to establish to what extent their composition and bioactivities are dependent on maturation.
29912150	0	43	theme	Radical	31:37	arg1	Properties					50:59	In Vitro Anti-Inflammatory and Radical Scavenging Properties	0:59	In Vitro Anti-Inflammatory and Radical Scavenging Properties of Chinotto (Citrus myrtifolia Raf.)	0:96	In Vitro Anti-Inflammatory and Radical Scavenging Properties of Chinotto (Citrus myrtifolia Raf.)
29912150	3	44	from	China	187:191	arg1	native					175:180	native	175:180	native	175:180	is a widely diffused plant native from China and its fruits have a wide-spread use in confectionary and drinks.
29912150	6	45	theme	genes	789:793	arg1	expression					762:771	the expression	758:771	the expression of inflammatory genes, cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), cytokines, including interleukin-1&beta; (IL-1&beta;) and interleukin-6 (IL-6)	758:942	Essential oil from half ripe chinotto (CEO2) reduced the production of nitric oxide (NO) and the expression of inflammatory genes, cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), cytokines, including interleukin-1&beta; (IL-1&beta;) and interleukin-6 (IL-6), and chemokine monocyte chemotactic protein-1 (MCP-1) by lipopolysaccharide (LPS)-stimulated RAW264,7 macrophages.
29912150	6	45	theme	genes	789:793	arg1	MCP-1					991:995	MCP-1	991:995	MCP-1	991:995	Essential oil from half ripe chinotto (CEO2) reduced the production of nitric oxide (NO) and the expression of inflammatory genes, cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), cytokines, including interleukin-1&beta; (IL-1&beta;) and interleukin-6 (IL-6), and chemokine monocyte chemotactic protein-1 (MCP-1) by lipopolysaccharide (LPS)-stimulated RAW264,7 macrophages.
29912150	6	45	theme	genes	789:793	arg1	production					722:731	the production	718:731	the production of nitric oxide (NO)	718:752	Essential oil from half ripe chinotto (CEO2) reduced the production of nitric oxide (NO) and the expression of inflammatory genes, cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), cytokines, including interleukin-1&beta; (IL-1&beta;) and interleukin-6 (IL-6), and chemokine monocyte chemotactic protein-1 (MCP-1) by lipopolysaccharide (LPS)-stimulated RAW264,7 macrophages.
29912150	6	45	theme	genes	789:793	arg1	protein-1					980:988	chemokine monocyte chemotactic protein-1	949:988	chemokine monocyte chemotactic protein-1 (MCP-1)	949:996	Essential oil from half ripe chinotto (CEO2) reduced the production of nitric oxide (NO) and the expression of inflammatory genes, cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), cytokines, including interleukin-1&beta; (IL-1&beta;) and interleukin-6 (IL-6), and chemokine monocyte chemotactic protein-1 (MCP-1) by lipopolysaccharide (LPS)-stimulated RAW264,7 macrophages.
29912150	4	46	theme	bergamia	407:414	arg1	bergamot					390:397	the taxonomically related bergamot	364:397	the taxonomically related bergamot (Citrus bergamia Risso)	364:421	Remarkably, only little has been reported thus far on its bioactive properties, in contrast to those of the taxonomically related bergamot (Citrus bergamia Risso).
29912150	4	46	theme	bergamia	407:414	arg1	Risso					416:420	Citrus bergamia Risso	400:420	Citrus bergamia Risso	400:420	Remarkably, only little has been reported thus far on its bioactive properties, in contrast to those of the taxonomically related bergamot (Citrus bergamia Risso).
29912150	8	47	theme	diammonium	1377:1386	arg1	ABTS					1394:1397	ABTS	1394:1397	ABTS	1394:1397	Moreover, CEO2 showed high radical scavenging activity measured as Trolox equivalents (TE) against both 2,2&prime;-diphenyl-1-picrylhydrazyl (DPPH) and 2,2&prime;-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS).
29912150	8	47	theme	diammonium	1377:1386	arg1	salt					1388:1391	-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt	1326:1391	both 2,2&prime;-diphenyl-1-picrylhydrazyl (DPPH) and 2,2&prime;-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS)	1263:1398	Moreover, CEO2 showed high radical scavenging activity measured as Trolox equivalents (TE) against both 2,2&prime;-diphenyl-1-picrylhydrazyl (DPPH) and 2,2&prime;-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS).
29912150	5	48	theme	radical	504:510	arg1	properties					523:532	potential in vitro anti-inflammatory and radical scavenging properties	463:532	potential in vitro anti-inflammatory and radical scavenging properties of chinotto essential oils (CEOs)	463:566	The present study aimed to investigate potential in vitro anti-inflammatory and radical scavenging properties of chinotto essential oils (CEOs) and to establish to what extent their composition and bioactivities are dependent on maturation.
29912150	6	49	theme	inducible	825:833	arg1	genes					789:793	inflammatory genes	776:793	inflammatory genes	776:793	Essential oil from half ripe chinotto (CEO2) reduced the production of nitric oxide (NO) and the expression of inflammatory genes, cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), cytokines, including interleukin-1&beta; (IL-1&beta;) and interleukin-6 (IL-6), and chemokine monocyte chemotactic protein-1 (MCP-1) by lipopolysaccharide (LPS)-stimulated RAW264,7 macrophages.
29912150	6	49	theme	inducible	825:833	arg1	interleukin-1&beta					886:903	interleukin-1&beta;	886:904	interleukin-1&beta; (IL-1&beta;)	886:917	Essential oil from half ripe chinotto (CEO2) reduced the production of nitric oxide (NO) and the expression of inflammatory genes, cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), cytokines, including interleukin-1&beta; (IL-1&beta;) and interleukin-6 (IL-6), and chemokine monocyte chemotactic protein-1 (MCP-1) by lipopolysaccharide (LPS)-stimulated RAW264,7 macrophages.
29912150	6	49	theme	inducible	825:833	arg1	interleukin-6					923:935	interleukin-6	923:935	interleukin-6 (IL-6)	923:942	Essential oil from half ripe chinotto (CEO2) reduced the production of nitric oxide (NO) and the expression of inflammatory genes, cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), cytokines, including interleukin-1&beta; (IL-1&beta;) and interleukin-6 (IL-6), and chemokine monocyte chemotactic protein-1 (MCP-1) by lipopolysaccharide (LPS)-stimulated RAW264,7 macrophages.
29912150	6	49	theme	inducible	825:833	arg1	iNOS					858:861	iNOS	858:861	iNOS	858:861	Essential oil from half ripe chinotto (CEO2) reduced the production of nitric oxide (NO) and the expression of inflammatory genes, cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), cytokines, including interleukin-1&beta; (IL-1&beta;) and interleukin-6 (IL-6), and chemokine monocyte chemotactic protein-1 (MCP-1) by lipopolysaccharide (LPS)-stimulated RAW264,7 macrophages.
29912150	6	49	theme	inducible	825:833	arg1	synthase					848:855	inducible nitric oxide synthase	825:855	inducible nitric oxide synthase (iNOS)	825:862	Essential oil from half ripe chinotto (CEO2) reduced the production of nitric oxide (NO) and the expression of inflammatory genes, cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), cytokines, including interleukin-1&beta; (IL-1&beta;) and interleukin-6 (IL-6), and chemokine monocyte chemotactic protein-1 (MCP-1) by lipopolysaccharide (LPS)-stimulated RAW264,7 macrophages.
29912150	8	50	theme	radical	1191:1197	arg1	activity					1210:1217	high radical scavenging activity	1186:1217	high radical scavenging activity measured as Trolox equivalents (TE) against both 2,2&prime;-diphenyl-1-picrylhydrazyl (DPPH) and 2,2&prime;-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS)	1186:1398	Moreover, CEO2 showed high radical scavenging activity measured as Trolox equivalents (TE) against both 2,2&prime;-diphenyl-1-picrylhydrazyl (DPPH) and 2,2&prime;-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS).
29912150	9	51	theme	fruit	1484:1488	arg1	part					1471:1474	a valuable part	1460:1474	a valuable part of this fruit	1460:1488	These findings show that chinotto essential oil represents a valuable part of this fruit and warrants further in vivo studies to validate its anti-inflammatory potential.
29912150	5	52	theme	scavenging	512:521	arg1	properties					523:532	potential in vitro anti-inflammatory and radical scavenging properties	463:532	potential in vitro anti-inflammatory and radical scavenging properties of chinotto essential oils (CEOs)	463:566	The present study aimed to investigate potential in vitro anti-inflammatory and radical scavenging properties of chinotto essential oils (CEOs) and to establish to what extent their composition and bioactivities are dependent on maturation.
29912150	8	53	theme	-azino-bis	1326:1335	arg1	ABTS					1394:1397	ABTS	1394:1397	ABTS	1394:1397	Moreover, CEO2 showed high radical scavenging activity measured as Trolox equivalents (TE) against both 2,2&prime;-diphenyl-1-picrylhydrazyl (DPPH) and 2,2&prime;-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS).
29912150	8	53	theme	-azino-bis	1326:1335	arg1	salt					1388:1391	-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt	1326:1391	both 2,2&prime;-diphenyl-1-picrylhydrazyl (DPPH) and 2,2&prime;-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS)	1263:1398	Moreover, CEO2 showed high radical scavenging activity measured as Trolox equivalents (TE) against both 2,2&prime;-diphenyl-1-picrylhydrazyl (DPPH) and 2,2&prime;-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS).
29912150	9	54	theme	in	1511:1512	arg1	studies					1519:1525	further in vivo studies	1503:1525	further in vivo studies	1503:1525	These findings show that chinotto essential oil represents a valuable part of this fruit and warrants further in vivo studies to validate its anti-inflammatory potential.
29912150	6	55	theme	nitric	736:741	arg1	NO					750:751	NO	750:751	NO	750:751	Essential oil from half ripe chinotto (CEO2) reduced the production of nitric oxide (NO) and the expression of inflammatory genes, cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), cytokines, including interleukin-1&beta; (IL-1&beta;) and interleukin-6 (IL-6), and chemokine monocyte chemotactic protein-1 (MCP-1) by lipopolysaccharide (LPS)-stimulated RAW264,7 macrophages.
29912150	6	55	theme	nitric	736:741	arg1	oxide					743:747	nitric oxide	736:747	nitric oxide (NO)	736:752	Essential oil from half ripe chinotto (CEO2) reduced the production of nitric oxide (NO) and the expression of inflammatory genes, cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), cytokines, including interleukin-1&beta; (IL-1&beta;) and interleukin-6 (IL-6), and chemokine monocyte chemotactic protein-1 (MCP-1) by lipopolysaccharide (LPS)-stimulated RAW264,7 macrophages.
29912150	7	56	theme	main	1139:1142	arg1	components					1144:1153	the main components	1135:1153	the main components in CEO2	1135:1161	Limonene, linalool, linalyl acetate, and &gamma;-terpinene were found to be the main components in CEO2.
29912150	7	56	theme	main	1139:1142	arg1	-terpinene					1107:1116	&gamma;-terpinene	1100:1116	&gamma;-terpinene	1100:1116	Limonene, linalool, linalyl acetate, and &gamma;-terpinene were found to be the main components in CEO2.
29912150	7	56	theme	main	1139:1142	arg1	linalool					1069:1076	linalool	1069:1076	linalool	1069:1076	Limonene, linalool, linalyl acetate, and &gamma;-terpinene were found to be the main components in CEO2.
29912150	7	56	theme	main	1139:1142	arg1	acetate					1087:1093	linalyl acetate	1079:1093	linalyl acetate	1079:1093	Limonene, linalool, linalyl acetate, and &gamma;-terpinene were found to be the main components in CEO2.
29912150	7	56	theme	main	1139:1142	arg1	Limonene					1059:1066	Limonene	1059:1066	Limonene	1059:1066	Limonene, linalool, linalyl acetate, and &gamma;-terpinene were found to be the main components in CEO2.
29912150	3	57	contain	have	208:211	arg2	use					227:229	a wide-spread use	213:229	a wide-spread use in confectionary and drinks	213:257	is a widely diffused plant native from China and its fruits have a wide-spread use in confectionary and drinks.
29912150	3	57	contain	have	208:211	arg1	fruits					201:206	its fruits	197:206	its fruits	197:206	is a widely diffused plant native from China and its fruits have a wide-spread use in confectionary and drinks.
29912150	7	58	theme	&gamma	1100:1105	arg1	components					1144:1153	the main components	1135:1153	the main components in CEO2	1135:1161	Limonene, linalool, linalyl acetate, and &gamma;-terpinene were found to be the main components in CEO2.
29912150	7	58	theme	&gamma	1100:1105	arg1	-terpinene					1107:1116	&gamma;-terpinene	1100:1116	&gamma;-terpinene	1100:1116	Limonene, linalool, linalyl acetate, and &gamma;-terpinene were found to be the main components in CEO2.
29912150	7	58	theme	&gamma	1100:1105	arg1	acetate					1087:1093	linalyl acetate	1079:1093	linalyl acetate	1079:1093	Limonene, linalool, linalyl acetate, and &gamma;-terpinene were found to be the main components in CEO2.
29912150	7	58	theme	&gamma	1100:1105	arg1	Limonene					1059:1066	Limonene	1059:1066	Limonene	1059:1066	Limonene, linalool, linalyl acetate, and &gamma;-terpinene were found to be the main components in CEO2.
29912150	7	58	theme	&gamma	1100:1105	arg1	linalool					1069:1076	linalool	1069:1076	linalool	1069:1076	Limonene, linalool, linalyl acetate, and &gamma;-terpinene were found to be the main components in CEO2.
29912150	5	59	theme	potential	463:471	arg1	properties					523:532	potential in vitro anti-inflammatory and radical scavenging properties	463:532	potential in vitro anti-inflammatory and radical scavenging properties of chinotto essential oils (CEOs)	463:566	The present study aimed to investigate potential in vitro anti-inflammatory and radical scavenging properties of chinotto essential oils (CEOs) and to establish to what extent their composition and bioactivities are dependent on maturation.
29912150	4	60	theme	related	382:388	arg1	bergamot					390:397	the taxonomically related bergamot	364:397	the taxonomically related bergamot (Citrus bergamia Risso)	364:421	Remarkably, only little has been reported thus far on its bioactive properties, in contrast to those of the taxonomically related bergamot (Citrus bergamia Risso).
29912150	4	60	theme	related	382:388	arg1	Risso					416:420	Citrus bergamia Risso	400:420	Citrus bergamia Risso	400:420	Remarkably, only little has been reported thus far on its bioactive properties, in contrast to those of the taxonomically related bergamot (Citrus bergamia Risso).
29912150	9	61	theme	further	1503:1509	arg1	studies					1519:1525	further in vivo studies	1503:1525	further in vivo studies	1503:1525	These findings show that chinotto essential oil represents a valuable part of this fruit and warrants further in vivo studies to validate its anti-inflammatory potential.
29912150	6	62	theme	ripe	689:692	arg1	CEO2					704:707	CEO2	704:707	CEO2	704:707	Essential oil from half ripe chinotto (CEO2) reduced the production of nitric oxide (NO) and the expression of inflammatory genes, cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), cytokines, including interleukin-1&beta; (IL-1&beta;) and interleukin-6 (IL-6), and chemokine monocyte chemotactic protein-1 (MCP-1) by lipopolysaccharide (LPS)-stimulated RAW264,7 macrophages.
29912150	6	62	theme	ripe	689:692	arg1	chinotto					694:701	half ripe chinotto	684:701	half ripe chinotto (CEO2)	684:708	Essential oil from half ripe chinotto (CEO2) reduced the production of nitric oxide (NO) and the expression of inflammatory genes, cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), cytokines, including interleukin-1&beta; (IL-1&beta;) and interleukin-6 (IL-6), and chemokine monocyte chemotactic protein-1 (MCP-1) by lipopolysaccharide (LPS)-stimulated RAW264,7 macrophages.
29912150	8	63	theme	high	1186:1189	arg1	activity					1210:1217	high radical scavenging activity	1186:1217	high radical scavenging activity measured as Trolox equivalents (TE) against both 2,2&prime;-diphenyl-1-picrylhydrazyl (DPPH) and 2,2&prime;-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS)	1186:1398	Moreover, CEO2 showed high radical scavenging activity measured as Trolox equivalents (TE) against both 2,2&prime;-diphenyl-1-picrylhydrazyl (DPPH) and 2,2&prime;-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS).
29912150	0	64	theme	Chinotto	64:71	arg1	Properties					50:59	In Vitro Anti-Inflammatory and Radical Scavenging Properties	0:59	In Vitro Anti-Inflammatory and Radical Scavenging Properties of Chinotto (Citrus myrtifolia Raf.)	0:96	In Vitro Anti-Inflammatory and Radical Scavenging Properties of Chinotto (Citrus myrtifolia Raf.)
29912150	0	65	dep	In	0:1	arg1	Vitro					3:7	Vitro	3:7	Vitro	3:7	In Vitro Anti-Inflammatory and Radical Scavenging Properties of Chinotto (Citrus myrtifolia Raf.)
29912150	6	66	theme	half	684:687	arg1	CEO2					704:707	CEO2	704:707	CEO2	704:707	Essential oil from half ripe chinotto (CEO2) reduced the production of nitric oxide (NO) and the expression of inflammatory genes, cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), cytokines, including interleukin-1&beta; (IL-1&beta;) and interleukin-6 (IL-6), and chemokine monocyte chemotactic protein-1 (MCP-1) by lipopolysaccharide (LPS)-stimulated RAW264,7 macrophages.
29912150	6	66	theme	half	684:687	arg1	chinotto					694:701	half ripe chinotto	684:701	half ripe chinotto (CEO2)	684:708	Essential oil from half ripe chinotto (CEO2) reduced the production of nitric oxide (NO) and the expression of inflammatory genes, cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), cytokines, including interleukin-1&beta; (IL-1&beta;) and interleukin-6 (IL-6), and chemokine monocyte chemotactic protein-1 (MCP-1) by lipopolysaccharide (LPS)-stimulated RAW264,7 macrophages.
29912150	8	67	dep	2,2&prime	1268:1276	arg1	ABTS					1394:1397	ABTS	1394:1397	ABTS	1394:1397	Moreover, CEO2 showed high radical scavenging activity measured as Trolox equivalents (TE) against both 2,2&prime;-diphenyl-1-picrylhydrazyl (DPPH) and 2,2&prime;-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS).
29912150	8	67	dep	2,2&prime	1268:1276	arg1	DPPH					1306:1309	DPPH	1306:1309	DPPH	1306:1309	Moreover, CEO2 showed high radical scavenging activity measured as Trolox equivalents (TE) against both 2,2&prime;-diphenyl-1-picrylhydrazyl (DPPH) and 2,2&prime;-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS).
29912150	8	67	dep	2,2&prime	1268:1276	arg1	salt					1388:1391	-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt	1326:1391	both 2,2&prime;-diphenyl-1-picrylhydrazyl (DPPH) and 2,2&prime;-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS)	1263:1398	Moreover, CEO2 showed high radical scavenging activity measured as Trolox equivalents (TE) against both 2,2&prime;-diphenyl-1-picrylhydrazyl (DPPH) and 2,2&prime;-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS).
29912150	8	67	dep	2,2&prime	1268:1276	arg1	-diphenyl-1-picrylhydrazyl					1278:1303	-diphenyl-1-picrylhydrazyl	1278:1303	-diphenyl-1-picrylhydrazyl (DPPH)	1278:1310	Moreover, CEO2 showed high radical scavenging activity measured as Trolox equivalents (TE) against both 2,2&prime;-diphenyl-1-picrylhydrazyl (DPPH) and 2,2&prime;-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS).
29912150	8	67	dep	2,2&prime	1268:1276	arg1	2,2&prime					1316:1324	2,2&prime	1316:1324	2,2&prime	1316:1324	Moreover, CEO2 showed high radical scavenging activity measured as Trolox equivalents (TE) against both 2,2&prime;-diphenyl-1-picrylhydrazyl (DPPH) and 2,2&prime;-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS).
29458478	4	0	theme	aerobic	291:297	arg1	strains					278:284	The three strains	268:284	The three strains	268:284	The three strains were aerobic, Gram-stain-positive, catalase-positive, non-sporulating and non-motile bacilli and exhibited unique biochemical profiles distinguishable from closely related Tsukamurella species.
29458478	4	0	theme	aerobic	291:297	arg1	bacilli					371:377	aerobic, Gram-stain-positive, catalase-positive, non-sporulating and non-motile bacilli	291:377	aerobic, Gram-stain-positive, catalase-positive, non-sporulating and non-motile bacilli	291:377	The three strains were aerobic, Gram-stain-positive, catalase-positive, non-sporulating and non-motile bacilli and exhibited unique biochemical profiles distinguishable from closely related Tsukamurella species.
29458478	7	1	theme	 DNA-DNA	1267:1274	arg1	relatedness					1276:1286	≥95.2±4.8 % DNA-DNA relatedness	1256:1286	≥95.2±4.8 % DNA-DNA relatedness	1256:1286	DNA-DNA hybridization confirmed that they were distinct from other known species of the genus Tsukamurella(23.0±4.2 to 50.7±3.7 % DNA-DNA relatedness), of which HKU63T and HKU64 represented the same species (≥95.2±4.8 % DNA-DNA relatedness) while HKU65T represented another species.
29458478	7	1	theme	 DNA-DNA	1267:1274	arg1	species					1247:1253	the same species	1238:1253	the same species (≥95.2±4.8 % DNA-DNA relatedness)	1238:1287	DNA-DNA hybridization confirmed that they were distinct from other known species of the genus Tsukamurella(23.0±4.2 to 50.7±3.7 % DNA-DNA relatedness), of which HKU63T and HKU64 represented the same species (≥95.2±4.8 % DNA-DNA relatedness) while HKU65T represented another species.
29458478	5	2	theme	99.9 	682:686	arg1	%					687:687	%	687:687	%	687:687	16S rRNA gene sequence analysis revealed that the three strains shared identical sequences with each other, being most closely related to Tsukamurella tyrosinosolvens and Tsukamurella pulmonis, sharing 99.9 % sequence identity.
29458478	3	3	from	conjunctivitis	239:252	arg1	Kong					262:265	Kong	262:265	Kong	262:265	Three bacterial strains, HKU63T, HKU64 and HKU65T, were isolated from the conjunctival swabs of three patients with conjunctivitis in Hong Kong.
29458478	7	4	from	species	1121:1127	arg1	distinct					1095:1102	distinct	1095:1102	distinct	1095:1102	DNA-DNA hybridization confirmed that they were distinct from other known species of the genus Tsukamurella(23.0±4.2 to 50.7±3.7 % DNA-DNA relatedness), of which HKU63T and HKU64 represented the same species (≥95.2±4.8 % DNA-DNA relatedness) while HKU65T represented another species.
29458478	10	5	theme	novel	1584:1588	arg1	species					1590:1596	A novel species	1582:1596	A novel species	1582:1596	A novel species, Tsukamurella ocularis sp.
29458478	5	6	theme	sequence	689:696	arg1	identity					698:705	99.9 % sequence identity	682:705	99.9 % sequence identity	682:705	16S rRNA gene sequence analysis revealed that the three strains shared identical sequences with each other, being most closely related to Tsukamurella tyrosinosolvens and Tsukamurella pulmonis, sharing 99.9 % sequence identity.
29458478	4	7	theme	unique	393:398	arg1	profiles					412:419	unique biochemical profiles	393:419	unique biochemical profiles distinguishable from closely related Tsukamurella species	393:477	The three strains were aerobic, Gram-stain-positive, catalase-positive, non-sporulating and non-motile bacilli and exhibited unique biochemical profiles distinguishable from closely related Tsukamurella species.
29458478	6	8	theme	nucleotide	803:812	arg1	identity					823:830	100 % nucleotide sequence identity	797:830	100 % nucleotide sequence identity between HKU63T and HKU64, 94.2-97.0 % nucleotide sequence identities between HKU63T/HKU64 and HKU65T	797:931	Sequence analysis of three additional housekeeping genes, groEL, secA and rpoB, revealed 100 % nucleotide sequence identity between HKU63T and HKU64, 94.2-97.0 % nucleotide sequence identities between HKU63T/HKU64 and HKU65T and the three strains shared 82.9-98.9 % sequence identities with other currently recognized Tsukamurella species.
29458478	9	9	theme	strains	1477:1483	arg1	71.3±1.9					1515:1522	71.3±1.9	1515:1522	71.3±1.9	1515:1522	The G+C content of strains HKU63T, HKU64 and HKU65T were 71.3±1.9, 71.3±2.0 and 71.2±2.3 mol% (mean±sd; n=3), respectively.
29458478	9	9	theme	strains	1477:1483	arg1	G+C content					1462:1472	The G+C content	1458:1472	The G+C content of strains HKU63T, HKU64 and HKU65T	1458:1508	The G+C content of strains HKU63T, HKU64 and HKU65T were 71.3±1.9, 71.3±2.0 and 71.2±2.3 mol% (mean±sd; n=3), respectively.
29458478	4	10	theme	non-sporulating	340:354	arg1	strains					278:284	The three strains	268:284	The three strains	268:284	The three strains were aerobic, Gram-stain-positive, catalase-positive, non-sporulating and non-motile bacilli and exhibited unique biochemical profiles distinguishable from closely related Tsukamurella species.
29458478	4	10	theme	non-sporulating	340:354	arg1	bacilli					371:377	aerobic, Gram-stain-positive, catalase-positive, non-sporulating and non-motile bacilli	291:377	aerobic, Gram-stain-positive, catalase-positive, non-sporulating and non-motile bacilli	291:377	The three strains were aerobic, Gram-stain-positive, catalase-positive, non-sporulating and non-motile bacilli and exhibited unique biochemical profiles distinguishable from closely related Tsukamurella species.
29458478	10	11	theme	ocularis	1612:1619	arg1	sp					1621:1622	Tsukamurella ocularis sp	1599:1622	Tsukamurella ocularis sp	1599:1622	A novel species, Tsukamurella ocularis sp.
29458478	7	12	theme	genus	1136:1140	arg1	Tsukamurella					1142:1153	the genus Tsukamurella	1132:1153	the genus Tsukamurella(23.0±4.2 to 50.7±3.7 % DNA-DNA relatedness)	1132:1197	DNA-DNA hybridization confirmed that they were distinct from other known species of the genus Tsukamurella(23.0±4.2 to 50.7±3.7 % DNA-DNA relatedness), of which HKU63T and HKU64 represented the same species (≥95.2±4.8 % DNA-DNA relatedness) while HKU65T represented another species.
29458478	7	12	theme	genus	1136:1140	arg1	relatedness					1186:1196	23.0±4.2 to 50.7±3.7 % DNA-DNA relatedness	1155:1196	23.0±4.2 to 50.7±3.7 % DNA-DNA relatedness	1155:1196	DNA-DNA hybridization confirmed that they were distinct from other known species of the genus Tsukamurella(23.0±4.2 to 50.7±3.7 % DNA-DNA relatedness), of which HKU63T and HKU64 represented the same species (≥95.2±4.8 % DNA-DNA relatedness) while HKU65T represented another species.
29458478	6	13	theme	100 	797:800	arg1	%					801:801	%	801:801	%	801:801	Sequence analysis of three additional housekeeping genes, groEL, secA and rpoB, revealed 100 % nucleotide sequence identity between HKU63T and HKU64, 94.2-97.0 % nucleotide sequence identities between HKU63T/HKU64 and HKU65T and the three strains shared 82.9-98.9 % sequence identities with other currently recognized Tsukamurella species.
29458478	5	14	theme	16S	480:482	arg1	rRNA					484:487	16S rRNA	480:487	16S rRNA gene sequence analysis	480:510	16S rRNA gene sequence analysis revealed that the three strains shared identical sequences with each other, being most closely related to Tsukamurella tyrosinosolvens and Tsukamurella pulmonis, sharing 99.9 % sequence identity.
29458478	7	15	theme	%	1176:1176	arg1	Tsukamurella					1142:1153	the genus Tsukamurella	1132:1153	the genus Tsukamurella(23.0±4.2 to 50.7±3.7 % DNA-DNA relatedness)	1132:1197	DNA-DNA hybridization confirmed that they were distinct from other known species of the genus Tsukamurella(23.0±4.2 to 50.7±3.7 % DNA-DNA relatedness), of which HKU63T and HKU64 represented the same species (≥95.2±4.8 % DNA-DNA relatedness) while HKU65T represented another species.
29458478	7	15	theme	%	1176:1176	arg1	relatedness					1186:1196	23.0±4.2 to 50.7±3.7 % DNA-DNA relatedness	1155:1196	23.0±4.2 to 50.7±3.7 % DNA-DNA relatedness	1155:1196	DNA-DNA hybridization confirmed that they were distinct from other known species of the genus Tsukamurella(23.0±4.2 to 50.7±3.7 % DNA-DNA relatedness), of which HKU63T and HKU64 represented the same species (≥95.2±4.8 % DNA-DNA relatedness) while HKU65T represented another species.
29458478	6	16	theme	other	999:1003	arg1	species					1039:1045	other currently recognized Tsukamurella species	999:1045	other currently recognized Tsukamurella species	999:1045	Sequence analysis of three additional housekeeping genes, groEL, secA and rpoB, revealed 100 % nucleotide sequence identity between HKU63T and HKU64, 94.2-97.0 % nucleotide sequence identities between HKU63T/HKU64 and HKU65T and the three strains shared 82.9-98.9 % sequence identities with other currently recognized Tsukamurella species.
29458478	4	17	theme	non-motile	360:369	arg1	strains					278:284	The three strains	268:284	The three strains	268:284	The three strains were aerobic, Gram-stain-positive, catalase-positive, non-sporulating and non-motile bacilli and exhibited unique biochemical profiles distinguishable from closely related Tsukamurella species.
29458478	4	17	theme	non-motile	360:369	arg1	bacilli					371:377	aerobic, Gram-stain-positive, catalase-positive, non-sporulating and non-motile bacilli	291:377	aerobic, Gram-stain-positive, catalase-positive, non-sporulating and non-motile bacilli	291:377	The three strains were aerobic, Gram-stain-positive, catalase-positive, non-sporulating and non-motile bacilli and exhibited unique biochemical profiles distinguishable from closely related Tsukamurella species.
29458478	11	18	theme	Tsukamurella	1781:1792	arg1	sp					1802:1803	Tsukamurella hominis sp	1781:1803	Tsukamurella hominis sp	1781:1803	nov. is proposed to accommodate strains HKU63T and HKU64, with HKU63T (=JCM 31969T=DSM 105034T) designated as the type strain whilst another novel species, Tsukamurella hominis sp.
29458478	11	18	theme	Tsukamurella	1781:1792	arg1	HKU63T					1688:1693	HKU63T	1688:1693	HKU63T (=JCM 31969T=DSM 105034T) designated as the type strain whilst another novel species	1688:1778	nov. is proposed to accommodate strains HKU63T and HKU64, with HKU63T (=JCM 31969T=DSM 105034T) designated as the type strain whilst another novel species, Tsukamurella hominis sp.
29458478	4	19	from	species	471:477	arg1	distinguishable					421:435	distinguishable	421:435	distinguishable	421:435	The three strains were aerobic, Gram-stain-positive, catalase-positive, non-sporulating and non-motile bacilli and exhibited unique biochemical profiles distinguishable from closely related Tsukamurella species.
29458478	5	20	theme	gene	489:492	arg1	analysis					503:510	16S rRNA gene sequence analysis	480:510	16S rRNA gene sequence analysis	480:510	16S rRNA gene sequence analysis revealed that the three strains shared identical sequences with each other, being most closely related to Tsukamurella tyrosinosolvens and Tsukamurella pulmonis, sharing 99.9 % sequence identity.
29458478	12	21	theme	31971T=DSM	1910:1919	arg1	105036T					1921:1927	=JCM 31971T=DSM 105036T	1905:1927	=JCM 31971T=DSM 105036T	1905:1927	nov., is proposed to accommodate the third strain, HKU65T, which is designated as the type strain (=JCM 31971T=DSM 105036T).
29458478	12	21	theme	31971T=DSM	1910:1919	arg1	strain					1897:1902	the type strain	1888:1902	the type strain (=JCM 31971T=DSM 105036T)	1888:1928	nov., is proposed to accommodate the third strain, HKU65T, which is designated as the type strain (=JCM 31971T=DSM 105036T).
29458478	4	22	theme	related	450:456	arg1	species					471:477	closely related Tsukamurella species	442:477	closely related Tsukamurella species	442:477	The three strains were aerobic, Gram-stain-positive, catalase-positive, non-sporulating and non-motile bacilli and exhibited unique biochemical profiles distinguishable from closely related Tsukamurella species.
29458478	7	23	theme	known	1115:1119	arg1	species					1121:1127	other known species	1109:1127	other known species	1109:1127	DNA-DNA hybridization confirmed that they were distinct from other known species of the genus Tsukamurella(23.0±4.2 to 50.7±3.7 % DNA-DNA relatedness), of which HKU63T and HKU64 represented the same species (≥95.2±4.8 % DNA-DNA relatedness) while HKU65T represented another species.
29458478	6	24	theme	%	972:972	arg1	identities					983:992	82.9-98.9 % sequence identities	962:992	82.9-98.9 % sequence identities	962:992	Sequence analysis of three additional housekeeping genes, groEL, secA and rpoB, revealed 100 % nucleotide sequence identity between HKU63T and HKU64, 94.2-97.0 % nucleotide sequence identities between HKU63T/HKU64 and HKU65T and the three strains shared 82.9-98.9 % sequence identities with other currently recognized Tsukamurella species.
29458478	8	25	theme	Fatty	1331:1335	arg1	acid					1337:1340	Fatty acid	1331:1340	Fatty acid	1331:1340	Fatty acid, mycolic acid, cell-wall sugar and peptidoglycan analyses showed that they were typical of members of Tsukamurella.
29458478	7	26	theme	DNA-DNA	1048:1054	arg1	hybridization					1056:1068	DNA-DNA hybridization	1048:1068	DNA-DNA hybridization	1048:1068	DNA-DNA hybridization confirmed that they were distinct from other known species of the genus Tsukamurella(23.0±4.2 to 50.7±3.7 % DNA-DNA relatedness), of which HKU63T and HKU64 represented the same species (≥95.2±4.8 % DNA-DNA relatedness) while HKU65T represented another species.
29458478	1	27	dep	sp	56:57	arg1	hominis					48:54	nov. and Tsukamurella hominis	26:54	nov. and Tsukamurella hominis	26:54	nov. and Tsukamurella hominis sp.
29458478	9	28	theme	71.2±2.3 mol	1538:1549	arg1	71.3±1.9					1515:1522	71.3±1.9	1515:1522	71.3±1.9	1515:1522	The G+C content of strains HKU63T, HKU64 and HKU65T were 71.3±1.9, 71.3±2.0 and 71.2±2.3 mol% (mean±sd; n=3), respectively.
29458478	9	28	theme	71.2±2.3 mol	1538:1549	arg1	mean±sd					1553:1559	mean±sd	1553:1559	mean±sd	1553:1559	The G+C content of strains HKU63T, HKU64 and HKU65T were 71.3±1.9, 71.3±2.0 and 71.2±2.3 mol% (mean±sd; n=3), respectively.
29458478	9	28	theme	71.2±2.3 mol	1538:1549	arg1	%					1550:1550	71.3±2.0 and 71.2±2.3 mol%	1525:1550	71.3±2.0 and 71.2±2.3 mol% (mean±sd; n=3)	1525:1565	The G+C content of strains HKU63T, HKU64 and HKU65T were 71.3±1.9, 71.3±2.0 and 71.2±2.3 mol% (mean±sd; n=3), respectively.
29458478	8	29	theme	acid	1351:1354	arg1	analyses					1391:1398	Fatty acid, mycolic acid, cell-wall sugar and peptidoglycan analyses	1331:1398	Fatty acid, mycolic acid, cell-wall sugar and peptidoglycan analyses	1331:1398	Fatty acid, mycolic acid, cell-wall sugar and peptidoglycan analyses showed that they were typical of members of Tsukamurella.
29458478	7	30	theme	%	1266:1266	arg1	relatedness					1276:1286	≥95.2±4.8 % DNA-DNA relatedness	1256:1286	≥95.2±4.8 % DNA-DNA relatedness	1256:1286	DNA-DNA hybridization confirmed that they were distinct from other known species of the genus Tsukamurella(23.0±4.2 to 50.7±3.7 % DNA-DNA relatedness), of which HKU63T and HKU64 represented the same species (≥95.2±4.8 % DNA-DNA relatedness) while HKU65T represented another species.
29458478	7	30	theme	%	1266:1266	arg1	species					1247:1253	the same species	1238:1253	the same species (≥95.2±4.8 % DNA-DNA relatedness)	1238:1287	DNA-DNA hybridization confirmed that they were distinct from other known species of the genus Tsukamurella(23.0±4.2 to 50.7±3.7 % DNA-DNA relatedness), of which HKU63T and HKU64 represented the same species (≥95.2±4.8 % DNA-DNA relatedness) while HKU65T represented another species.
29458478	8	31	theme	cell-wall	1357:1365	arg1	sugar					1367:1371	cell-wall sugar	1357:1371	cell-wall sugar	1357:1371	Fatty acid, mycolic acid, cell-wall sugar and peptidoglycan analyses showed that they were typical of members of Tsukamurella.
29458478	7	32	theme	same	1242:1245	arg1	relatedness					1276:1286	≥95.2±4.8 % DNA-DNA relatedness	1256:1286	≥95.2±4.8 % DNA-DNA relatedness	1256:1286	DNA-DNA hybridization confirmed that they were distinct from other known species of the genus Tsukamurella(23.0±4.2 to 50.7±3.7 % DNA-DNA relatedness), of which HKU63T and HKU64 represented the same species (≥95.2±4.8 % DNA-DNA relatedness) while HKU65T represented another species.
29458478	7	32	theme	same	1242:1245	arg1	species					1247:1253	the same species	1238:1253	the same species (≥95.2±4.8 % DNA-DNA relatedness)	1238:1287	DNA-DNA hybridization confirmed that they were distinct from other known species of the genus Tsukamurella(23.0±4.2 to 50.7±3.7 % DNA-DNA relatedness), of which HKU63T and HKU64 represented the same species (≥95.2±4.8 % DNA-DNA relatedness) while HKU65T represented another species.
29458478	6	33	theme	recognized	1015:1024	arg1	species					1039:1045	other currently recognized Tsukamurella species	999:1045	other currently recognized Tsukamurella species	999:1045	Sequence analysis of three additional housekeeping genes, groEL, secA and rpoB, revealed 100 % nucleotide sequence identity between HKU63T and HKU64, 94.2-97.0 % nucleotide sequence identities between HKU63T/HKU64 and HKU65T and the three strains shared 82.9-98.9 % sequence identities with other currently recognized Tsukamurella species.
29458478	6	34	theme	additional	735:744	arg1	groEL					766:770	groEL	766:770	groEL	766:770	Sequence analysis of three additional housekeeping genes, groEL, secA and rpoB, revealed 100 % nucleotide sequence identity between HKU63T and HKU64, 94.2-97.0 % nucleotide sequence identities between HKU63T/HKU64 and HKU65T and the three strains shared 82.9-98.9 % sequence identities with other currently recognized Tsukamurella species.
29458478	6	34	theme	additional	735:744	arg1	genes					759:763	three additional housekeeping genes	729:763	three additional housekeeping genes	729:763	Sequence analysis of three additional housekeeping genes, groEL, secA and rpoB, revealed 100 % nucleotide sequence identity between HKU63T and HKU64, 94.2-97.0 % nucleotide sequence identities between HKU63T/HKU64 and HKU65T and the three strains shared 82.9-98.9 % sequence identities with other currently recognized Tsukamurella species.
29458478	6	34	theme	additional	735:744	arg1	rpoB					782:785	rpoB	782:785	rpoB	782:785	Sequence analysis of three additional housekeeping genes, groEL, secA and rpoB, revealed 100 % nucleotide sequence identity between HKU63T and HKU64, 94.2-97.0 % nucleotide sequence identities between HKU63T/HKU64 and HKU65T and the three strains shared 82.9-98.9 % sequence identities with other currently recognized Tsukamurella species.
29458478	6	34	theme	additional	735:744	arg1	secA					773:776	secA	773:776	secA	773:776	Sequence analysis of three additional housekeeping genes, groEL, secA and rpoB, revealed 100 % nucleotide sequence identity between HKU63T and HKU64, 94.2-97.0 % nucleotide sequence identities between HKU63T/HKU64 and HKU65T and the three strains shared 82.9-98.9 % sequence identities with other currently recognized Tsukamurella species.
29458478	9	35	dep	mean±sd	1553:1559	arg1	n=3					1562:1564	n=3	1562:1564	n=3	1562:1564	The G+C content of strains HKU63T, HKU64 and HKU65T were 71.3±1.9, 71.3±2.0 and 71.2±2.3 mol% (mean±sd; n=3), respectively.
29458478	0	36	theme	Tsukamurella	0:11	arg1	sp					22:23	Tsukamurella ocularis sp	0:23	Tsukamurella ocularis sp.	0:24	Tsukamurella ocularis sp.
29458478	6	37	theme	Sequence	708:715	arg1	analysis					717:724	Sequence analysis	708:724	Sequence analysis of three additional housekeeping genes, groEL, secA and rpoB,	708:786	Sequence analysis of three additional housekeeping genes, groEL, secA and rpoB, revealed 100 % nucleotide sequence identity between HKU63T and HKU64, 94.2-97.0 % nucleotide sequence identities between HKU63T/HKU64 and HKU65T and the three strains shared 82.9-98.9 % sequence identities with other currently recognized Tsukamurella species.
29458478	11	38	dep	strains	1657:1663	arg1	strains					1657:1663	strains HKU63T and HKU64	1657:1680	strains HKU63T and HKU64	1657:1680	nov. is proposed to accommodate strains HKU63T and HKU64, with HKU63T (=JCM 31969T=DSM 105034T) designated as the type strain whilst another novel species, Tsukamurella hominis sp.
29458478	11	38	dep	strains	1657:1663	arg1	HKU64					1676:1680	HKU64	1676:1680	HKU64	1676:1680	nov. is proposed to accommodate strains HKU63T and HKU64, with HKU63T (=JCM 31969T=DSM 105034T) designated as the type strain whilst another novel species, Tsukamurella hominis sp.
29458478	11	38	dep	strains	1657:1663	arg1	HKU63T					1665:1670	HKU63T	1665:1670	HKU63T	1665:1670	nov. is proposed to accommodate strains HKU63T and HKU64, with HKU63T (=JCM 31969T=DSM 105034T) designated as the type strain whilst another novel species, Tsukamurella hominis sp.
29458478	11	39	theme	31969T=DSM	1701:1710	arg1	HKU63T					1688:1693	HKU63T	1688:1693	HKU63T (=JCM 31969T=DSM 105034T) designated as the type strain whilst another novel species	1688:1778	nov. is proposed to accommodate strains HKU63T and HKU64, with HKU63T (=JCM 31969T=DSM 105034T) designated as the type strain whilst another novel species, Tsukamurella hominis sp.
29458478	11	39	theme	31969T=DSM	1701:1710	arg1	105034T					1712:1718	=JCM 31969T=DSM 105034T	1696:1718	=JCM 31969T=DSM 105034T	1696:1718	nov. is proposed to accommodate strains HKU63T and HKU64, with HKU63T (=JCM 31969T=DSM 105034T) designated as the type strain whilst another novel species, Tsukamurella hominis sp.
29458478	6	40	theme	 nucleotide	869:879	arg1	identities					890:899	HKU64, 94.2-97.0 % nucleotide sequence identities	851:899	HKU64, 94.2-97.0 % nucleotide sequence identities	851:899	Sequence analysis of three additional housekeeping genes, groEL, secA and rpoB, revealed 100 % nucleotide sequence identity between HKU63T and HKU64, 94.2-97.0 % nucleotide sequence identities between HKU63T/HKU64 and HKU65T and the three strains shared 82.9-98.9 % sequence identities with other currently recognized Tsukamurella species.
29458478	6	41	dep	%	868:868	arg1	94.2-97.0 					858:867	94.2-97.0 	858:867	94.2-97.0 	858:867	Sequence analysis of three additional housekeeping genes, groEL, secA and rpoB, revealed 100 % nucleotide sequence identity between HKU63T and HKU64, 94.2-97.0 % nucleotide sequence identities between HKU63T/HKU64 and HKU65T and the three strains shared 82.9-98.9 % sequence identities with other currently recognized Tsukamurella species.
29458478	3	42	with	patients	225:232	arg1	conjunctivitis					239:252	conjunctivitis	239:252	conjunctivitis in Hong Kong	239:265	Three bacterial strains, HKU63T, HKU64 and HKU65T, were isolated from the conjunctival swabs of three patients with conjunctivitis in Hong Kong.
29458478	6	43	theme	genes	759:763	arg1	analysis					717:724	Sequence analysis	708:724	Sequence analysis of three additional housekeeping genes, groEL, secA and rpoB,	708:786	Sequence analysis of three additional housekeeping genes, groEL, secA and rpoB, revealed 100 % nucleotide sequence identity between HKU63T and HKU64, 94.2-97.0 % nucleotide sequence identities between HKU63T/HKU64 and HKU65T and the three strains shared 82.9-98.9 % sequence identities with other currently recognized Tsukamurella species.
29458478	3	44	theme	conjunctival	197:208	arg1	swabs					210:214	the conjunctival swabs	193:214	the conjunctival swabs of three patients with conjunctivitis in Hong Kong	193:265	Three bacterial strains, HKU63T, HKU64 and HKU65T, were isolated from the conjunctival swabs of three patients with conjunctivitis in Hong Kong.
29458478	5	45	theme	%	687:687	arg1	identity					698:705	99.9 % sequence identity	682:705	99.9 % sequence identity	682:705	16S rRNA gene sequence analysis revealed that the three strains shared identical sequences with each other, being most closely related to Tsukamurella tyrosinosolvens and Tsukamurella pulmonis, sharing 99.9 % sequence identity.
29458478	4	46	theme	biochemical	400:410	arg1	profiles					412:419	unique biochemical profiles	393:419	unique biochemical profiles distinguishable from closely related Tsukamurella species	393:477	The three strains were aerobic, Gram-stain-positive, catalase-positive, non-sporulating and non-motile bacilli and exhibited unique biochemical profiles distinguishable from closely related Tsukamurella species.
29458478	3	47	attach	isolated	179:186	arg2	strains					139:145	Three bacterial strains	123:145	Three bacterial strains	123:145	Three bacterial strains, HKU63T, HKU64 and HKU65T, were isolated from the conjunctival swabs of three patients with conjunctivitis in Hong Kong.
29458478	3	47	attach	isolated	179:186	arg2	HKU65T					166:171	HKU65T	166:171	HKU65T	166:171	Three bacterial strains, HKU63T, HKU64 and HKU65T, were isolated from the conjunctival swabs of three patients with conjunctivitis in Hong Kong.
29458478	3	47	attach	isolated	179:186	arg2	HKU64					156:160	HKU64	156:160	HKU64	156:160	Three bacterial strains, HKU63T, HKU64 and HKU65T, were isolated from the conjunctival swabs of three patients with conjunctivitis in Hong Kong.
29458478	3	47	attach	isolated	179:186	arg2	HKU63T					148:153	HKU63T	148:153	HKU63T	148:153	Three bacterial strains, HKU63T, HKU64 and HKU65T, were isolated from the conjunctival swabs of three patients with conjunctivitis in Hong Kong.
29458478	3	47	attach	isolated	179:186	arg1	swabs					210:214	the conjunctival swabs	193:214	the conjunctival swabs of three patients with conjunctivitis in Hong Kong	193:265	Three bacterial strains, HKU63T, HKU64 and HKU65T, were isolated from the conjunctival swabs of three patients with conjunctivitis in Hong Kong.
29458478	7	48	theme	Tsukamurella	1142:1153	arg1	species					1121:1127	other known species	1109:1127	other known species	1109:1127	DNA-DNA hybridization confirmed that they were distinct from other known species of the genus Tsukamurella(23.0±4.2 to 50.7±3.7 % DNA-DNA relatedness), of which HKU63T and HKU64 represented the same species (≥95.2±4.8 % DNA-DNA relatedness) while HKU65T represented another species.
29458478	6	49	theme	sequence	814:821	arg1	identity					823:830	100 % nucleotide sequence identity	797:830	100 % nucleotide sequence identity between HKU63T and HKU64, 94.2-97.0 % nucleotide sequence identities between HKU63T/HKU64 and HKU65T	797:931	Sequence analysis of three additional housekeeping genes, groEL, secA and rpoB, revealed 100 % nucleotide sequence identity between HKU63T and HKU64, 94.2-97.0 % nucleotide sequence identities between HKU63T/HKU64 and HKU65T and the three strains shared 82.9-98.9 % sequence identities with other currently recognized Tsukamurella species.
29458478	3	50	theme	patients	225:232	arg1	swabs					210:214	the conjunctival swabs	193:214	the conjunctival swabs of three patients with conjunctivitis in Hong Kong	193:265	Three bacterial strains, HKU63T, HKU64 and HKU65T, were isolated from the conjunctival swabs of three patients with conjunctivitis in Hong Kong.
29458478	10	51	dep	sp	1621:1622	arg1	species					1590:1596	A novel species	1582:1596	A novel species	1582:1596	A novel species, Tsukamurella ocularis sp.
29458478	6	52	theme	%	801:801	arg1	identity					823:830	100 % nucleotide sequence identity	797:830	100 % nucleotide sequence identity between HKU63T and HKU64, 94.2-97.0 % nucleotide sequence identities between HKU63T/HKU64 and HKU65T	797:931	Sequence analysis of three additional housekeeping genes, groEL, secA and rpoB, revealed 100 % nucleotide sequence identity between HKU63T and HKU64, 94.2-97.0 % nucleotide sequence identities between HKU63T/HKU64 and HKU65T and the three strains shared 82.9-98.9 % sequence identities with other currently recognized Tsukamurella species.
29458478	2	53	from	conjunctivitis	94:107	arg1	Kong					117:120	Kong	117:120	Kong	117:120	nov., isolated from patients with conjunctivitis in Hong Kong.
29458478	10	54	theme	Tsukamurella	1599:1610	arg1	sp					1621:1622	Tsukamurella ocularis sp	1599:1622	Tsukamurella ocularis sp	1599:1622	A novel species, Tsukamurella ocularis sp.
29458478	8	55	theme	members	1433:1439	arg1	typical					1422:1428	typical	1422:1428	typical	1422:1428	Fatty acid, mycolic acid, cell-wall sugar and peptidoglycan analyses showed that they were typical of members of Tsukamurella.
29458478	5	56	theme	rRNA	484:487	arg1	analysis					503:510	16S rRNA gene sequence analysis	480:510	16S rRNA gene sequence analysis	480:510	16S rRNA gene sequence analysis revealed that the three strains shared identical sequences with each other, being most closely related to Tsukamurella tyrosinosolvens and Tsukamurella pulmonis, sharing 99.9 % sequence identity.
29458478	4	57	theme	Tsukamurella	458:469	arg1	species					471:477	closely related Tsukamurella species	442:477	closely related Tsukamurella species	442:477	The three strains were aerobic, Gram-stain-positive, catalase-positive, non-sporulating and non-motile bacilli and exhibited unique biochemical profiles distinguishable from closely related Tsukamurella species.
29458478	11	58	theme	hominis	1794:1800	arg1	sp					1802:1803	Tsukamurella hominis sp	1781:1803	Tsukamurella hominis sp	1781:1803	nov. is proposed to accommodate strains HKU63T and HKU64, with HKU63T (=JCM 31969T=DSM 105034T) designated as the type strain whilst another novel species, Tsukamurella hominis sp.
29458478	11	58	theme	hominis	1794:1800	arg1	HKU63T					1688:1693	HKU63T	1688:1693	HKU63T (=JCM 31969T=DSM 105034T) designated as the type strain whilst another novel species	1688:1778	nov. is proposed to accommodate strains HKU63T and HKU64, with HKU63T (=JCM 31969T=DSM 105034T) designated as the type strain whilst another novel species, Tsukamurella hominis sp.
29458478	7	59	theme	other	1109:1113	arg1	species					1121:1127	other known species	1109:1127	other known species	1109:1127	DNA-DNA hybridization confirmed that they were distinct from other known species of the genus Tsukamurella(23.0±4.2 to 50.7±3.7 % DNA-DNA relatedness), of which HKU63T and HKU64 represented the same species (≥95.2±4.8 % DNA-DNA relatedness) while HKU65T represented another species.
29458478	5	60	theme	sequence	494:501	arg1	analysis					503:510	16S rRNA gene sequence analysis	480:510	16S rRNA gene sequence analysis	480:510	16S rRNA gene sequence analysis revealed that the three strains shared identical sequences with each other, being most closely related to Tsukamurella tyrosinosolvens and Tsukamurella pulmonis, sharing 99.9 % sequence identity.
29458478	12	61	theme	=JCM	1905:1908	arg1	105036T					1921:1927	=JCM 31971T=DSM 105036T	1905:1927	=JCM 31971T=DSM 105036T	1905:1927	nov., is proposed to accommodate the third strain, HKU65T, which is designated as the type strain (=JCM 31971T=DSM 105036T).
29458478	12	61	theme	=JCM	1905:1908	arg1	strain					1897:1902	the type strain	1888:1902	the type strain (=JCM 31971T=DSM 105036T)	1888:1928	nov., is proposed to accommodate the third strain, HKU65T, which is designated as the type strain (=JCM 31971T=DSM 105036T).
29458478	6	62	theme	 sequence	973:981	arg1	identities					983:992	82.9-98.9 % sequence identities	962:992	82.9-98.9 % sequence identities	962:992	Sequence analysis of three additional housekeeping genes, groEL, secA and rpoB, revealed 100 % nucleotide sequence identity between HKU63T and HKU64, 94.2-97.0 % nucleotide sequence identities between HKU63T/HKU64 and HKU65T and the three strains shared 82.9-98.9 % sequence identities with other currently recognized Tsukamurella species.
29458478	7	63	dep	50.7±3.7 	1167:1175	arg1	to					1164:1165	to	1164:1165	to	1164:1165	DNA-DNA hybridization confirmed that they were distinct from other known species of the genus Tsukamurella(23.0±4.2 to 50.7±3.7 % DNA-DNA relatedness), of which HKU63T and HKU64 represented the same species (≥95.2±4.8 % DNA-DNA relatedness) while HKU65T represented another species.
29458478	6	64	theme	82.9-98.9 	962:971	arg1	%					972:972	%	972:972	%	972:972	Sequence analysis of three additional housekeeping genes, groEL, secA and rpoB, revealed 100 % nucleotide sequence identity between HKU63T and HKU64, 94.2-97.0 % nucleotide sequence identities between HKU63T/HKU64 and HKU65T and the three strains shared 82.9-98.9 % sequence identities with other currently recognized Tsukamurella species.
29458478	4	65	theme	distinguishable	421:435	arg1	profiles					412:419	unique biochemical profiles	393:419	unique biochemical profiles distinguishable from closely related Tsukamurella species	393:477	The three strains were aerobic, Gram-stain-positive, catalase-positive, non-sporulating and non-motile bacilli and exhibited unique biochemical profiles distinguishable from closely related Tsukamurella species.
29458478	9	66	theme	71.3±2.0 and	1525:1536	arg1	71.3±1.9					1515:1522	71.3±1.9	1515:1522	71.3±1.9	1515:1522	The G+C content of strains HKU63T, HKU64 and HKU65T were 71.3±1.9, 71.3±2.0 and 71.2±2.3 mol% (mean±sd; n=3), respectively.
29458478	9	66	theme	71.3±2.0 and	1525:1536	arg1	mean±sd					1553:1559	mean±sd	1553:1559	mean±sd	1553:1559	The G+C content of strains HKU63T, HKU64 and HKU65T were 71.3±1.9, 71.3±2.0 and 71.2±2.3 mol% (mean±sd; n=3), respectively.
29458478	9	66	theme	71.3±2.0 and	1525:1536	arg1	%					1550:1550	71.3±2.0 and 71.2±2.3 mol%	1525:1550	71.3±2.0 and 71.2±2.3 mol% (mean±sd; n=3)	1525:1565	The G+C content of strains HKU63T, HKU64 and HKU65T were 71.3±1.9, 71.3±2.0 and 71.2±2.3 mol% (mean±sd; n=3), respectively.
29458478	3	67	theme	bacterial	129:137	arg1	HKU65T					166:171	HKU65T	166:171	HKU65T	166:171	Three bacterial strains, HKU63T, HKU64 and HKU65T, were isolated from the conjunctival swabs of three patients with conjunctivitis in Hong Kong.
29458478	3	67	theme	bacterial	129:137	arg1	HKU64					156:160	HKU64	156:160	HKU64	156:160	Three bacterial strains, HKU63T, HKU64 and HKU65T, were isolated from the conjunctival swabs of three patients with conjunctivitis in Hong Kong.
29458478	3	67	theme	bacterial	129:137	arg1	HKU63T					148:153	HKU63T	148:153	HKU63T	148:153	Three bacterial strains, HKU63T, HKU64 and HKU65T, were isolated from the conjunctival swabs of three patients with conjunctivitis in Hong Kong.
29458478	3	67	theme	bacterial	129:137	arg1	strains					139:145	Three bacterial strains	123:145	Three bacterial strains	123:145	Three bacterial strains, HKU63T, HKU64 and HKU65T, were isolated from the conjunctival swabs of three patients with conjunctivitis in Hong Kong.
29458478	8	68	theme	acid	1337:1340	arg1	analyses					1391:1398	Fatty acid, mycolic acid, cell-wall sugar and peptidoglycan analyses	1331:1398	Fatty acid, mycolic acid, cell-wall sugar and peptidoglycan analyses	1331:1398	Fatty acid, mycolic acid, cell-wall sugar and peptidoglycan analyses showed that they were typical of members of Tsukamurella.
29458478	7	69	theme	≥95.2±4.8 	1256:1265	arg1	relatedness					1276:1286	≥95.2±4.8 % DNA-DNA relatedness	1256:1286	≥95.2±4.8 % DNA-DNA relatedness	1256:1286	DNA-DNA hybridization confirmed that they were distinct from other known species of the genus Tsukamurella(23.0±4.2 to 50.7±3.7 % DNA-DNA relatedness), of which HKU63T and HKU64 represented the same species (≥95.2±4.8 % DNA-DNA relatedness) while HKU65T represented another species.
29458478	7	69	theme	≥95.2±4.8 	1256:1265	arg1	species					1247:1253	the same species	1238:1253	the same species (≥95.2±4.8 % DNA-DNA relatedness)	1238:1287	DNA-DNA hybridization confirmed that they were distinct from other known species of the genus Tsukamurella(23.0±4.2 to 50.7±3.7 % DNA-DNA relatedness), of which HKU63T and HKU64 represented the same species (≥95.2±4.8 % DNA-DNA relatedness) while HKU65T represented another species.
29458478	2	70	with	patients	80:87	arg1	conjunctivitis					94:107	conjunctivitis	94:107	conjunctivitis in Hong Kong	94:120	nov., isolated from patients with conjunctivitis in Hong Kong.
29458478	8	71	theme	mycolic	1343:1349	arg1	acid					1351:1354	mycolic acid	1343:1354	mycolic acid	1343:1354	Fatty acid, mycolic acid, cell-wall sugar and peptidoglycan analyses showed that they were typical of members of Tsukamurella.
29458478	11	72	theme	type	1739:1742	arg1	strain					1744:1749	the type strain	1735:1749	the type strain whilst another novel species	1735:1778	nov. is proposed to accommodate strains HKU63T and HKU64, with HKU63T (=JCM 31969T=DSM 105034T) designated as the type strain whilst another novel species, Tsukamurella hominis sp.
29458478	12	73	theme	type	1892:1895	arg1	105036T					1921:1927	=JCM 31971T=DSM 105036T	1905:1927	=JCM 31971T=DSM 105036T	1905:1927	nov., is proposed to accommodate the third strain, HKU65T, which is designated as the type strain (=JCM 31971T=DSM 105036T).
29458478	12	73	theme	type	1892:1895	arg1	strain					1897:1902	the type strain	1888:1902	the type strain (=JCM 31971T=DSM 105036T)	1888:1928	nov., is proposed to accommodate the third strain, HKU65T, which is designated as the type strain (=JCM 31971T=DSM 105036T).
29458478	12	73	theme	type	1892:1895	arg1	strain					1849:1854	the third strain	1839:1854	the third strain	1839:1854	nov., is proposed to accommodate the third strain, HKU65T, which is designated as the type strain (=JCM 31971T=DSM 105036T).
29458478	5	74	theme	identical	551:559	arg1	sequences					561:569	identical sequences	551:569	identical sequences	551:569	16S rRNA gene sequence analysis revealed that the three strains shared identical sequences with each other, being most closely related to Tsukamurella tyrosinosolvens and Tsukamurella pulmonis, sharing 99.9 % sequence identity.
29458478	6	75	theme	Tsukamurella	1026:1037	arg1	species					1039:1045	other currently recognized Tsukamurella species	999:1045	other currently recognized Tsukamurella species	999:1045	Sequence analysis of three additional housekeeping genes, groEL, secA and rpoB, revealed 100 % nucleotide sequence identity between HKU63T and HKU64, 94.2-97.0 % nucleotide sequence identities between HKU63T/HKU64 and HKU65T and the three strains shared 82.9-98.9 % sequence identities with other currently recognized Tsukamurella species.
29458478	8	76	theme	sugar	1367:1371	arg1	analyses					1391:1398	Fatty acid, mycolic acid, cell-wall sugar and peptidoglycan analyses	1331:1398	Fatty acid, mycolic acid, cell-wall sugar and peptidoglycan analyses	1331:1398	Fatty acid, mycolic acid, cell-wall sugar and peptidoglycan analyses showed that they were typical of members of Tsukamurella.
29458478	6	77	theme	housekeeping	746:757	arg1	groEL					766:770	groEL	766:770	groEL	766:770	Sequence analysis of three additional housekeeping genes, groEL, secA and rpoB, revealed 100 % nucleotide sequence identity between HKU63T and HKU64, 94.2-97.0 % nucleotide sequence identities between HKU63T/HKU64 and HKU65T and the three strains shared 82.9-98.9 % sequence identities with other currently recognized Tsukamurella species.
29458478	6	77	theme	housekeeping	746:757	arg1	genes					759:763	three additional housekeeping genes	729:763	three additional housekeeping genes	729:763	Sequence analysis of three additional housekeeping genes, groEL, secA and rpoB, revealed 100 % nucleotide sequence identity between HKU63T and HKU64, 94.2-97.0 % nucleotide sequence identities between HKU63T/HKU64 and HKU65T and the three strains shared 82.9-98.9 % sequence identities with other currently recognized Tsukamurella species.
29458478	6	77	theme	housekeeping	746:757	arg1	rpoB					782:785	rpoB	782:785	rpoB	782:785	Sequence analysis of three additional housekeeping genes, groEL, secA and rpoB, revealed 100 % nucleotide sequence identity between HKU63T and HKU64, 94.2-97.0 % nucleotide sequence identities between HKU63T/HKU64 and HKU65T and the three strains shared 82.9-98.9 % sequence identities with other currently recognized Tsukamurella species.
29458478	6	77	theme	housekeeping	746:757	arg1	secA					773:776	secA	773:776	secA	773:776	Sequence analysis of three additional housekeeping genes, groEL, secA and rpoB, revealed 100 % nucleotide sequence identity between HKU63T and HKU64, 94.2-97.0 % nucleotide sequence identities between HKU63T/HKU64 and HKU65T and the three strains shared 82.9-98.9 % sequence identities with other currently recognized Tsukamurella species.
29458478	11	78	theme	novel	1766:1770	arg1	species					1772:1778	another novel species	1758:1778	another novel species	1758:1778	nov. is proposed to accommodate strains HKU63T and HKU64, with HKU63T (=JCM 31969T=DSM 105034T) designated as the type strain whilst another novel species, Tsukamurella hominis sp.
29458478	8	79	theme	peptidoglycan	1377:1389	arg1	analyses					1391:1398	Fatty acid, mycolic acid, cell-wall sugar and peptidoglycan analyses	1331:1398	Fatty acid, mycolic acid, cell-wall sugar and peptidoglycan analyses	1331:1398	Fatty acid, mycolic acid, cell-wall sugar and peptidoglycan analyses showed that they were typical of members of Tsukamurella.
29458478	0	80	theme	ocularis	13:20	arg1	sp					22:23	Tsukamurella ocularis sp	0:23	Tsukamurella ocularis sp.	0:24	Tsukamurella ocularis sp.
29458478	11	81	theme	=JCM	1696:1699	arg1	HKU63T					1688:1693	HKU63T	1688:1693	HKU63T (=JCM 31969T=DSM 105034T) designated as the type strain whilst another novel species	1688:1778	nov. is proposed to accommodate strains HKU63T and HKU64, with HKU63T (=JCM 31969T=DSM 105034T) designated as the type strain whilst another novel species, Tsukamurella hominis sp.
29458478	11	81	theme	=JCM	1696:1699	arg1	105034T					1712:1718	=JCM 31969T=DSM 105034T	1696:1718	=JCM 31969T=DSM 105034T	1696:1718	nov. is proposed to accommodate strains HKU63T and HKU64, with HKU63T (=JCM 31969T=DSM 105034T) designated as the type strain whilst another novel species, Tsukamurella hominis sp.
29458478	6	82	theme	sequence	881:888	arg1	identities					890:899	HKU64, 94.2-97.0 % nucleotide sequence identities	851:899	HKU64, 94.2-97.0 % nucleotide sequence identities	851:899	Sequence analysis of three additional housekeeping genes, groEL, secA and rpoB, revealed 100 % nucleotide sequence identity between HKU63T and HKU64, 94.2-97.0 % nucleotide sequence identities between HKU63T/HKU64 and HKU65T and the three strains shared 82.9-98.9 % sequence identities with other currently recognized Tsukamurella species.
29458478	7	83	theme	 DNA-DNA	1177:1184	arg1	Tsukamurella					1142:1153	the genus Tsukamurella	1132:1153	the genus Tsukamurella(23.0±4.2 to 50.7±3.7 % DNA-DNA relatedness)	1132:1197	DNA-DNA hybridization confirmed that they were distinct from other known species of the genus Tsukamurella(23.0±4.2 to 50.7±3.7 % DNA-DNA relatedness), of which HKU63T and HKU64 represented the same species (≥95.2±4.8 % DNA-DNA relatedness) while HKU65T represented another species.
29458478	7	83	theme	 DNA-DNA	1177:1184	arg1	relatedness					1186:1196	23.0±4.2 to 50.7±3.7 % DNA-DNA relatedness	1155:1196	23.0±4.2 to 50.7±3.7 % DNA-DNA relatedness	1155:1196	DNA-DNA hybridization confirmed that they were distinct from other known species of the genus Tsukamurella(23.0±4.2 to 50.7±3.7 % DNA-DNA relatedness), of which HKU63T and HKU64 represented the same species (≥95.2±4.8 % DNA-DNA relatedness) while HKU65T represented another species.
29458478	4	84	theme	Gram-stain-positive	300:318	arg1	strains					278:284	The three strains	268:284	The three strains	268:284	The three strains were aerobic, Gram-stain-positive, catalase-positive, non-sporulating and non-motile bacilli and exhibited unique biochemical profiles distinguishable from closely related Tsukamurella species.
29458478	4	84	theme	Gram-stain-positive	300:318	arg1	bacilli					371:377	aerobic, Gram-stain-positive, catalase-positive, non-sporulating and non-motile bacilli	291:377	aerobic, Gram-stain-positive, catalase-positive, non-sporulating and non-motile bacilli	291:377	The three strains were aerobic, Gram-stain-positive, catalase-positive, non-sporulating and non-motile bacilli and exhibited unique biochemical profiles distinguishable from closely related Tsukamurella species.
29458478	6	85	dep	identities	890:899	arg1	%					868:868	%	868:868	%	868:868	Sequence analysis of three additional housekeeping genes, groEL, secA and rpoB, revealed 100 % nucleotide sequence identity between HKU63T and HKU64, 94.2-97.0 % nucleotide sequence identities between HKU63T/HKU64 and HKU65T and the three strains shared 82.9-98.9 % sequence identities with other currently recognized Tsukamurella species.
29458478	9	86	dep	strains	1477:1483	arg1	HKU63T					1485:1490	HKU63T	1485:1490	HKU63T	1485:1490	The G+C content of strains HKU63T, HKU64 and HKU65T were 71.3±1.9, 71.3±2.0 and 71.2±2.3 mol% (mean±sd; n=3), respectively.
29458478	9	86	dep	strains	1477:1483	arg1	HKU65T					1503:1508	HKU65T	1503:1508	HKU65T	1503:1508	The G+C content of strains HKU63T, HKU64 and HKU65T were 71.3±1.9, 71.3±2.0 and 71.2±2.3 mol% (mean±sd; n=3), respectively.
29458478	9	86	dep	strains	1477:1483	arg1	strains					1477:1483	strains HKU63T, HKU64 and HKU65T	1477:1508	strains HKU63T, HKU64 and HKU65T	1477:1508	The G+C content of strains HKU63T, HKU64 and HKU65T were 71.3±1.9, 71.3±2.0 and 71.2±2.3 mol% (mean±sd; n=3), respectively.
29458478	9	86	dep	strains	1477:1483	arg1	HKU64					1493:1497	HKU64	1493:1497	HKU64	1493:1497	The G+C content of strains HKU63T, HKU64 and HKU65T were 71.3±1.9, 71.3±2.0 and 71.2±2.3 mol% (mean±sd; n=3), respectively.
29458478	12	87	theme	third	1843:1847	arg1	HKU65T					1857:1862	HKU65T	1857:1862	HKU65T	1857:1862	nov., is proposed to accommodate the third strain, HKU65T, which is designated as the type strain (=JCM 31971T=DSM 105036T).
29458478	12	87	theme	third	1843:1847	arg1	strain					1897:1902	the type strain	1888:1902	the type strain (=JCM 31971T=DSM 105036T)	1888:1928	nov., is proposed to accommodate the third strain, HKU65T, which is designated as the type strain (=JCM 31971T=DSM 105036T).
29458478	12	87	theme	third	1843:1847	arg1	strain					1849:1854	the third strain	1839:1854	the third strain	1839:1854	nov., is proposed to accommodate the third strain, HKU65T, which is designated as the type strain (=JCM 31971T=DSM 105036T).
29458478	1	88	theme	nov.	26:29	arg1	hominis					48:54	nov. and Tsukamurella hominis	26:54	nov. and Tsukamurella hominis	26:54	nov. and Tsukamurella hominis sp.
29458478	4	89	theme	catalase-positive	321:337	arg1	strains					278:284	The three strains	268:284	The three strains	268:284	The three strains were aerobic, Gram-stain-positive, catalase-positive, non-sporulating and non-motile bacilli and exhibited unique biochemical profiles distinguishable from closely related Tsukamurella species.
29458478	4	89	theme	catalase-positive	321:337	arg1	bacilli					371:377	aerobic, Gram-stain-positive, catalase-positive, non-sporulating and non-motile bacilli	291:377	aerobic, Gram-stain-positive, catalase-positive, non-sporulating and non-motile bacilli	291:377	The three strains were aerobic, Gram-stain-positive, catalase-positive, non-sporulating and non-motile bacilli and exhibited unique biochemical profiles distinguishable from closely related Tsukamurella species.
29458478	5	90	dep	tyrosinosolvens	631:645	arg1	pulmonis					664:671	pulmonis	664:671	pulmonis	664:671	16S rRNA gene sequence analysis revealed that the three strains shared identical sequences with each other, being most closely related to Tsukamurella tyrosinosolvens and Tsukamurella pulmonis, sharing 99.9 % sequence identity.
29458478	8	91	theme	Tsukamurella	1444:1455	arg1	members					1433:1439	members	1433:1439	members of Tsukamurella	1433:1455	Fatty acid, mycolic acid, cell-wall sugar and peptidoglycan analyses showed that they were typical of members of Tsukamurella.
29458478	1	92	theme	Tsukamurella	35:46	arg1	hominis					48:54	nov. and Tsukamurella hominis	26:54	nov. and Tsukamurella hominis	26:54	nov. and Tsukamurella hominis sp.
29725246	4	0	theme	15-min	1212:1217	arg1	exercise					1228:1235	a 15-min swimming exercise	1210:1235	a 15-min swimming exercise	1210:1235	Exercise performance was evaluated by forelimb grip strength and time to exhaustion, as well as changes in body composition and anti-fatigue levels after a 15-min swimming exercise.
29725246	5	1	theme	glucose	1303:1309	arg1	responses					1266:1274	Fatigue-related biochemical responses	1238:1274	Fatigue-related biochemical responses of serum lactate, ammonia, glucose, creatine kinase (CK), and blood urea nitrogen (BUN)	1238:1362	Fatigue-related biochemical responses of serum lactate, ammonia, glucose, creatine kinase (CK), and blood urea nitrogen (BUN) were measured following the swimming exercise.
29725246	3	2	with	vehicle	911:917	arg1	training					1000:1007	WWBV training	995:1007	WWBV training (DHEA: 10.2 mg/kg; WBV: 5.6 Hz, 2 mm, 0.13 g)	995:1053	Methods: Male middle-aged C57BL/6 mice were divided into 3 groups (n = 8 per group) and treated for 4 weeks with the following: 1) Sedentary control (SC) with vehicle 2) DHEA supplementation (DHEA, 10.2 mg/kg) and 3) DHEA supplementation with WWBV training (DHEA: 10.2 mg/kg; WBV: 5.6 Hz, 2 mm, 0.13 g).
29725246	10	3	theme	elderly	2148:2154	arg1	populations					2156:2166	elderly populations	2148:2166	elderly populations	2148:2166	Conclusion: Taken together, our results suggest that combining the WWBV training program with DHEA supplementation could provide an anti-fatigue pharmacological effect for elderly populations.
29725246	7	4	theme	tissue	1645:1650	arg1	amount					1621:1626	the amount	1617:1626	the amount of white adipose tissue	1617:1650	Results: DHEA supplementation combined with WWBV training for 4 weeks significantly decreased the amount of white adipose tissue and increased the food and water intake.
29725246	7	4	theme	tissue	1645:1650	arg1	tissue					1645:1650	white adipose tissue	1631:1650	white adipose tissue	1631:1650	Results: DHEA supplementation combined with WWBV training for 4 weeks significantly decreased the amount of white adipose tissue and increased the food and water intake.
29725246	8	5	theme	WWBV+DHEA	1707:1715	arg1	supplementation					1717:1731	WWBV+DHEA supplementation	1707:1731	WWBV+DHEA supplementation	1707:1731	Additionally, WWBV+DHEA supplementation improved exercise performance, testosterone levels and glycogen contents of both liver and muscle.
29725246	5	6	theme	creatine	1312:1319	arg1	CK					1329:1330	CK	1329:1330	CK	1329:1330	Fatigue-related biochemical responses of serum lactate, ammonia, glucose, creatine kinase (CK), and blood urea nitrogen (BUN) were measured following the swimming exercise.
29725246	5	6	theme	creatine	1312:1319	arg1	kinase					1321:1326	creatine kinase	1312:1326	creatine kinase (CK)	1312:1331	Fatigue-related biochemical responses of serum lactate, ammonia, glucose, creatine kinase (CK), and blood urea nitrogen (BUN) were measured following the swimming exercise.
29725246	2	7	theme	physical	638:645	arg1	responses					675:683	physical fatigue-related biochemical responses	638:683	physical fatigue-related biochemical responses	638:683	Purpose: In the current study, we investigate the effects of Dehydroepiandrosterone (DHEA), one of the most abundant circulating steroids in humans and a precursor hormone, supplementation combined with a weight-loading whole-body vibration (WWBV) on exercise performance, physical fatigue-related biochemical responses and testosterone content in middle-aged 9 months old C57BL/6 mice.
29725246	7	8	theme	white	1631:1635	arg1	tissue					1645:1650	white adipose tissue	1631:1650	white adipose tissue	1631:1650	Results: DHEA supplementation combined with WWBV training for 4 weeks significantly decreased the amount of white adipose tissue and increased the food and water intake.
29725246	4	9	theme	anti-fatigue	1184:1195	arg1	levels					1197:1202	anti-fatigue levels	1184:1202	anti-fatigue levels after a 15-min swimming exercise	1184:1235	Exercise performance was evaluated by forelimb grip strength and time to exhaustion, as well as changes in body composition and anti-fatigue levels after a 15-min swimming exercise.
29725246	5	10	theme	lactate	1285:1291	arg1	responses					1266:1274	Fatigue-related biochemical responses	1238:1274	Fatigue-related biochemical responses of serum lactate, ammonia, glucose, creatine kinase (CK), and blood urea nitrogen (BUN)	1238:1362	Fatigue-related biochemical responses of serum lactate, ammonia, glucose, creatine kinase (CK), and blood urea nitrogen (BUN) were measured following the swimming exercise.
29725246	1	11	theme	Adequate	203:210	arg1	program					255:261	an optimal training program	235:261	an optimal training program	235:261	Background: Adequate nutritional intake and an optimal training program are important elements of any strategy to preserve or increase muscle mass and strength during aging.
29725246	1	11	theme	Adequate	203:210	arg1	intake					224:229	Adequate nutritional intake	203:229	Adequate nutritional intake	203:229	Background: Adequate nutritional intake and an optimal training program are important elements of any strategy to preserve or increase muscle mass and strength during aging.
29725246	1	11	theme	Adequate	203:210	arg1	elements					277:284	important elements	267:284	important elements of any strategy to preserve or increase muscle mass and strength during aging	267:362	Background: Adequate nutritional intake and an optimal training program are important elements of any strategy to preserve or increase muscle mass and strength during aging.
29725246	1	11	theme	Adequate	203:210	arg1	Background					191:200	Background	191:200	Background: Adequate nutritional intake and an optimal training program are important elements of any strategy to preserve or increase muscle mass and strength during aging.	191:363	Background: Adequate nutritional intake and an optimal training program are important elements of any strategy to preserve or increase muscle mass and strength during aging.
29725246	3	12	theme	DHEA	969:972	arg1	supplementation					974:988	DHEA supplementation	969:988	3) DHEA supplementation with WWBV training (DHEA: 10.2 mg/kg; WBV: 5.6 Hz, 2 mm, 0.13 g)	966:1053	Methods: Male middle-aged C57BL/6 mice were divided into 3 groups (n = 8 per group) and treated for 4 weeks with the following: 1) Sedentary control (SC) with vehicle 2) DHEA supplementation (DHEA, 10.2 mg/kg) and 3) DHEA supplementation with WWBV training (DHEA: 10.2 mg/kg; WBV: 5.6 Hz, 2 mm, 0.13 g).
29725246	3	13	theme	C57BL/6	778:784	arg1	mice					786:789	Male middle-aged C57BL/6 mice	761:789	Male middle-aged C57BL/6 mice	761:789	Methods: Male middle-aged C57BL/6 mice were divided into 3 groups (n = 8 per group) and treated for 4 weeks with the following: 1) Sedentary control (SC) with vehicle 2) DHEA supplementation (DHEA, 10.2 mg/kg) and 3) DHEA supplementation with WWBV training (DHEA: 10.2 mg/kg; WBV: 5.6 Hz, 2 mm, 0.13 g).
29725246	5	14	theme	ammonia	1294:1300	arg1	responses					1266:1274	Fatigue-related biochemical responses	1238:1274	Fatigue-related biochemical responses of serum lactate, ammonia, glucose, creatine kinase (CK), and blood urea nitrogen (BUN)	1238:1362	Fatigue-related biochemical responses of serum lactate, ammonia, glucose, creatine kinase (CK), and blood urea nitrogen (BUN) were measured following the swimming exercise.
29725246	10	15	theme	anti-fatigue	2108:2119	arg1	effect					2137:2142	an anti-fatigue pharmacological effect	2105:2142	an anti-fatigue pharmacological effect for elderly populations	2105:2166	Conclusion: Taken together, our results suggest that combining the WWBV training program with DHEA supplementation could provide an anti-fatigue pharmacological effect for elderly populations.
29725246	3	16	theme	Male	761:764	arg1	mice					786:789	Male middle-aged C57BL/6 mice	761:789	Male middle-aged C57BL/6 mice	761:789	Methods: Male middle-aged C57BL/6 mice were divided into 3 groups (n = 8 per group) and treated for 4 weeks with the following: 1) Sedentary control (SC) with vehicle 2) DHEA supplementation (DHEA, 10.2 mg/kg) and 3) DHEA supplementation with WWBV training (DHEA: 10.2 mg/kg; WBV: 5.6 Hz, 2 mm, 0.13 g).
29725246	0	17	theme	glycogen	145:152	arg1	storage					154:160	muscle glycogen storage	138:160	muscle glycogen storage	138:160	Dehydroepiandrosterone supplementation combined with Weight-Loading Whole-Body Vibration Training (WWBV) affects exercise performance and muscle glycogen storage in middle-aged C57BL/6 mice.
29725246	3	18	dep	groups	811:816	arg1	n					819:819	n = 8	819:823	n = 8	819:823	Methods: Male middle-aged C57BL/6 mice were divided into 3 groups (n = 8 per group) and treated for 4 weeks with the following: 1) Sedentary control (SC) with vehicle 2) DHEA supplementation (DHEA, 10.2 mg/kg) and 3) DHEA supplementation with WWBV training (DHEA: 10.2 mg/kg; WBV: 5.6 Hz, 2 mm, 0.13 g).
29725246	1	19	theme	training	246:253	arg1	program					255:261	an optimal training program	235:261	an optimal training program	235:261	Background: Adequate nutritional intake and an optimal training program are important elements of any strategy to preserve or increase muscle mass and strength during aging.
29725246	1	19	theme	training	246:253	arg1	elements					277:284	important elements	267:284	important elements of any strategy to preserve or increase muscle mass and strength during aging	267:362	Background: Adequate nutritional intake and an optimal training program are important elements of any strategy to preserve or increase muscle mass and strength during aging.
29725246	1	19	theme	training	246:253	arg1	intake					224:229	Adequate nutritional intake	203:229	Adequate nutritional intake	203:229	Background: Adequate nutritional intake and an optimal training program are important elements of any strategy to preserve or increase muscle mass and strength during aging.
29725246	1	19	theme	training	246:253	arg1	Background					191:200	Background	191:200	Background: Adequate nutritional intake and an optimal training program are important elements of any strategy to preserve or increase muscle mass and strength during aging.	191:363	Background: Adequate nutritional intake and an optimal training program are important elements of any strategy to preserve or increase muscle mass and strength during aging.
29725246	2	20	theme	old	734:736	arg1	mice					746:749	middle-aged 9 months old C57BL/6 mice	713:749	middle-aged 9 months old C57BL/6 mice	713:749	Purpose: In the current study, we investigate the effects of Dehydroepiandrosterone (DHEA), one of the most abundant circulating steroids in humans and a precursor hormone, supplementation combined with a weight-loading whole-body vibration (WWBV) on exercise performance, physical fatigue-related biochemical responses and testosterone content in middle-aged 9 months old C57BL/6 mice.
29725246	0	21	theme	C57BL/6	177:183	arg1	mice					185:188	middle-aged C57BL/6 mice	165:188	middle-aged C57BL/6 mice	165:188	Dehydroepiandrosterone supplementation combined with Weight-Loading Whole-Body Vibration Training (WWBV) affects exercise performance and muscle glycogen storage in middle-aged C57BL/6 mice.
29725246	2	22	theme	weight-loading	570:583	arg1	WWBV					607:610	WWBV	607:610	WWBV	607:610	Purpose: In the current study, we investigate the effects of Dehydroepiandrosterone (DHEA), one of the most abundant circulating steroids in humans and a precursor hormone, supplementation combined with a weight-loading whole-body vibration (WWBV) on exercise performance, physical fatigue-related biochemical responses and testosterone content in middle-aged 9 months old C57BL/6 mice.
29725246	2	22	theme	weight-loading	570:583	arg1	vibration					596:604	a weight-loading whole-body vibration	568:604	a weight-loading whole-body vibration (WWBV)	568:611	Purpose: In the current study, we investigate the effects of Dehydroepiandrosterone (DHEA), one of the most abundant circulating steroids in humans and a precursor hormone, supplementation combined with a weight-loading whole-body vibration (WWBV) on exercise performance, physical fatigue-related biochemical responses and testosterone content in middle-aged 9 months old C57BL/6 mice.
29725246	8	23	theme	testosterone	1764:1775	arg1	levels					1777:1782	testosterone levels	1764:1782	testosterone levels	1764:1782	Additionally, WWBV+DHEA supplementation improved exercise performance, testosterone levels and glycogen contents of both liver and muscle.
29725246	4	24	theme	Exercise	1056:1063	arg1	performance					1065:1075	Exercise performance	1056:1075	Exercise performance	1056:1075	Exercise performance was evaluated by forelimb grip strength and time to exhaustion, as well as changes in body composition and anti-fatigue levels after a 15-min swimming exercise.
29725246	9	25	theme	WWBV+DHEA	1832:1840	arg1	supplementation					1842:1856	WWBV+DHEA supplementation	1832:1856	WWBV+DHEA supplementation	1832:1856	WWBV+DHEA supplementation also decreased serum lactate, ammonia and BUN levels, while increasing glucose levels following the 15-min swim test.
29725246	3	26	dep	control	893:899	arg1	1					880:880	1	880:880	1	880:880	Methods: Male middle-aged C57BL/6 mice were divided into 3 groups (n = 8 per group) and treated for 4 weeks with the following: 1) Sedentary control (SC) with vehicle 2) DHEA supplementation (DHEA, 10.2 mg/kg) and 3) DHEA supplementation with WWBV training (DHEA: 10.2 mg/kg; WBV: 5.6 Hz, 2 mm, 0.13 g).
29725246	2	27	from	content	702:708	arg1	mice					746:749	middle-aged 9 months old C57BL/6 mice	713:749	middle-aged 9 months old C57BL/6 mice	713:749	Purpose: In the current study, we investigate the effects of Dehydroepiandrosterone (DHEA), one of the most abundant circulating steroids in humans and a precursor hormone, supplementation combined with a weight-loading whole-body vibration (WWBV) on exercise performance, physical fatigue-related biochemical responses and testosterone content in middle-aged 9 months old C57BL/6 mice.
29725246	2	28	theme	steroids	494:501	arg1	Dehydroepiandrosterone					426:447	Dehydroepiandrosterone	426:447	Dehydroepiandrosterone (DHEA)	426:454	Purpose: In the current study, we investigate the effects of Dehydroepiandrosterone (DHEA), one of the most abundant circulating steroids in humans and a precursor hormone, supplementation combined with a weight-loading whole-body vibration (WWBV) on exercise performance, physical fatigue-related biochemical responses and testosterone content in middle-aged 9 months old C57BL/6 mice.
29725246	2	28	theme	steroids	494:501	arg1	one					457:459	one	457:459	one	457:459	Purpose: In the current study, we investigate the effects of Dehydroepiandrosterone (DHEA), one of the most abundant circulating steroids in humans and a precursor hormone, supplementation combined with a weight-loading whole-body vibration (WWBV) on exercise performance, physical fatigue-related biochemical responses and testosterone content in middle-aged 9 months old C57BL/6 mice.
29725246	2	28	theme	steroids	494:501	arg1	supplementation					538:552	supplementation	538:552	supplementation combined with a weight-loading whole-body vibration (WWBV)	538:611	Purpose: In the current study, we investigate the effects of Dehydroepiandrosterone (DHEA), one of the most abundant circulating steroids in humans and a precursor hormone, supplementation combined with a weight-loading whole-body vibration (WWBV) on exercise performance, physical fatigue-related biochemical responses and testosterone content in middle-aged 9 months old C57BL/6 mice.
29725246	2	28	theme	steroids	494:501	arg1	steroids					494:501	the most abundant circulating steroids	464:501	the most abundant circulating steroids in humans	464:511	Purpose: In the current study, we investigate the effects of Dehydroepiandrosterone (DHEA), one of the most abundant circulating steroids in humans and a precursor hormone, supplementation combined with a weight-loading whole-body vibration (WWBV) on exercise performance, physical fatigue-related biochemical responses and testosterone content in middle-aged 9 months old C57BL/6 mice.
29725246	2	28	theme	steroids	494:501	arg1	hormone					529:535	a precursor hormone	517:535	a precursor hormone	517:535	Purpose: In the current study, we investigate the effects of Dehydroepiandrosterone (DHEA), one of the most abundant circulating steroids in humans and a precursor hormone, supplementation combined with a weight-loading whole-body vibration (WWBV) on exercise performance, physical fatigue-related biochemical responses and testosterone content in middle-aged 9 months old C57BL/6 mice.
29725246	9	29	theme	serum	1873:1877	arg1	lactate					1879:1885	serum lactate	1873:1885	serum lactate	1873:1885	WWBV+DHEA supplementation also decreased serum lactate, ammonia and BUN levels, while increasing glucose levels following the 15-min swim test.
29725246	0	30	from	performance	122:132	arg1	mice					185:188	middle-aged C57BL/6 mice	165:188	middle-aged C57BL/6 mice	165:188	Dehydroepiandrosterone supplementation combined with Weight-Loading Whole-Body Vibration Training (WWBV) affects exercise performance and muscle glycogen storage in middle-aged C57BL/6 mice.
29725246	2	31	theme	hormone	529:535	arg1	Dehydroepiandrosterone					426:447	Dehydroepiandrosterone	426:447	Dehydroepiandrosterone (DHEA)	426:454	Purpose: In the current study, we investigate the effects of Dehydroepiandrosterone (DHEA), one of the most abundant circulating steroids in humans and a precursor hormone, supplementation combined with a weight-loading whole-body vibration (WWBV) on exercise performance, physical fatigue-related biochemical responses and testosterone content in middle-aged 9 months old C57BL/6 mice.
29725246	2	31	theme	hormone	529:535	arg1	one					457:459	one	457:459	one	457:459	Purpose: In the current study, we investigate the effects of Dehydroepiandrosterone (DHEA), one of the most abundant circulating steroids in humans and a precursor hormone, supplementation combined with a weight-loading whole-body vibration (WWBV) on exercise performance, physical fatigue-related biochemical responses and testosterone content in middle-aged 9 months old C57BL/6 mice.
29725246	2	31	theme	hormone	529:535	arg1	supplementation					538:552	supplementation	538:552	supplementation combined with a weight-loading whole-body vibration (WWBV)	538:611	Purpose: In the current study, we investigate the effects of Dehydroepiandrosterone (DHEA), one of the most abundant circulating steroids in humans and a precursor hormone, supplementation combined with a weight-loading whole-body vibration (WWBV) on exercise performance, physical fatigue-related biochemical responses and testosterone content in middle-aged 9 months old C57BL/6 mice.
29725246	2	31	theme	hormone	529:535	arg1	steroids					494:501	the most abundant circulating steroids	464:501	the most abundant circulating steroids in humans	464:511	Purpose: In the current study, we investigate the effects of Dehydroepiandrosterone (DHEA), one of the most abundant circulating steroids in humans and a precursor hormone, supplementation combined with a weight-loading whole-body vibration (WWBV) on exercise performance, physical fatigue-related biochemical responses and testosterone content in middle-aged 9 months old C57BL/6 mice.
29725246	2	31	theme	hormone	529:535	arg1	hormone					529:535	a precursor hormone	517:535	a precursor hormone	517:535	Purpose: In the current study, we investigate the effects of Dehydroepiandrosterone (DHEA), one of the most abundant circulating steroids in humans and a precursor hormone, supplementation combined with a weight-loading whole-body vibration (WWBV) on exercise performance, physical fatigue-related biochemical responses and testosterone content in middle-aged 9 months old C57BL/6 mice.
29725246	5	32	theme	urea	1344:1347	arg1	BUN					1359:1361	BUN	1359:1361	BUN	1359:1361	Fatigue-related biochemical responses of serum lactate, ammonia, glucose, creatine kinase (CK), and blood urea nitrogen (BUN) were measured following the swimming exercise.
29725246	5	32	theme	urea	1344:1347	arg1	nitrogen					1349:1356	blood urea nitrogen	1338:1356	blood urea nitrogen (BUN)	1338:1362	Fatigue-related biochemical responses of serum lactate, ammonia, glucose, creatine kinase (CK), and blood urea nitrogen (BUN) were measured following the swimming exercise.
29725246	4	33	theme	grip	1103:1106	arg1	strength					1108:1115	forelimb grip strength	1094:1115	forelimb grip strength	1094:1115	Exercise performance was evaluated by forelimb grip strength and time to exhaustion, as well as changes in body composition and anti-fatigue levels after a 15-min swimming exercise.
29725246	0	34	theme	exercise	113:120	arg1	performance					122:132	exercise performance	113:132	exercise performance	113:132	Dehydroepiandrosterone supplementation combined with Weight-Loading Whole-Body Vibration Training (WWBV) affects exercise performance and muscle glycogen storage in middle-aged C57BL/6 mice.
29725246	2	35	theme	fatigue-related	647:661	arg1	responses					675:683	physical fatigue-related biochemical responses	638:683	physical fatigue-related biochemical responses	638:683	Purpose: In the current study, we investigate the effects of Dehydroepiandrosterone (DHEA), one of the most abundant circulating steroids in humans and a precursor hormone, supplementation combined with a weight-loading whole-body vibration (WWBV) on exercise performance, physical fatigue-related biochemical responses and testosterone content in middle-aged 9 months old C57BL/6 mice.
29725246	10	36	theme	pharmacological	2121:2135	arg1	effect					2137:2142	an anti-fatigue pharmacological effect	2105:2142	an anti-fatigue pharmacological effect for elderly populations	2105:2166	Conclusion: Taken together, our results suggest that combining the WWBV training program with DHEA supplementation could provide an anti-fatigue pharmacological effect for elderly populations.
29725246	0	37	theme	Dehydroepiandrosterone	0:21	arg1	supplementation					23:37	Dehydroepiandrosterone supplementation	0:37	Dehydroepiandrosterone supplementation combined with Weight-Loading Whole-Body Vibration Training (WWBV)	0:103	Dehydroepiandrosterone supplementation combined with Weight-Loading Whole-Body Vibration Training (WWBV) affects exercise performance and muscle glycogen storage in middle-aged C57BL/6 mice.
29725246	2	38	theme	testosterone	689:700	arg1	content					702:708	testosterone content	689:708	testosterone content	689:708	Purpose: In the current study, we investigate the effects of Dehydroepiandrosterone (DHEA), one of the most abundant circulating steroids in humans and a precursor hormone, supplementation combined with a weight-loading whole-body vibration (WWBV) on exercise performance, physical fatigue-related biochemical responses and testosterone content in middle-aged 9 months old C57BL/6 mice.
29725246	3	39	dep	training	1000:1007	arg1	DHEA					1010:1013	DHEA	1010:1013	DHEA	1010:1013	Methods: Male middle-aged C57BL/6 mice were divided into 3 groups (n = 8 per group) and treated for 4 weeks with the following: 1) Sedentary control (SC) with vehicle 2) DHEA supplementation (DHEA, 10.2 mg/kg) and 3) DHEA supplementation with WWBV training (DHEA: 10.2 mg/kg; WBV: 5.6 Hz, 2 mm, 0.13 g).
29725246	3	39	dep	training	1000:1007	arg1	g					1052:1052	0.13 g	1047:1052	0.13 g	1047:1052	Methods: Male middle-aged C57BL/6 mice were divided into 3 groups (n = 8 per group) and treated for 4 weeks with the following: 1) Sedentary control (SC) with vehicle 2) DHEA supplementation (DHEA, 10.2 mg/kg) and 3) DHEA supplementation with WWBV training (DHEA: 10.2 mg/kg; WBV: 5.6 Hz, 2 mm, 0.13 g).
29725246	2	40	theme	abundant	473:480	arg1	steroids					494:501	the most abundant circulating steroids	464:501	the most abundant circulating steroids in humans	464:511	Purpose: In the current study, we investigate the effects of Dehydroepiandrosterone (DHEA), one of the most abundant circulating steroids in humans and a precursor hormone, supplementation combined with a weight-loading whole-body vibration (WWBV) on exercise performance, physical fatigue-related biochemical responses and testosterone content in middle-aged 9 months old C57BL/6 mice.
29725246	9	41	theme	glucose	1929:1935	arg1	levels					1937:1942	glucose levels	1929:1942	glucose levels following the 15-min swim test	1929:1973	WWBV+DHEA supplementation also decreased serum lactate, ammonia and BUN levels, while increasing glucose levels following the 15-min swim test.
29725246	0	42	theme	Weight-Loading	53:66	arg1	WWBV					99:102	WWBV	99:102	WWBV	99:102	Dehydroepiandrosterone supplementation combined with Weight-Loading Whole-Body Vibration Training (WWBV) affects exercise performance and muscle glycogen storage in middle-aged C57BL/6 mice.
29725246	0	42	theme	Weight-Loading	53:66	arg1	Training					89:96	Weight-Loading Whole-Body Vibration Training	53:96	Weight-Loading Whole-Body Vibration Training (WWBV)	53:103	Dehydroepiandrosterone supplementation combined with Weight-Loading Whole-Body Vibration Training (WWBV) affects exercise performance and muscle glycogen storage in middle-aged C57BL/6 mice.
29725246	3	43	dep	DHEA	1010:1013	arg1	WBV					1028:1030	WBV	1028:1030	WBV	1028:1030	Methods: Male middle-aged C57BL/6 mice were divided into 3 groups (n = 8 per group) and treated for 4 weeks with the following: 1) Sedentary control (SC) with vehicle 2) DHEA supplementation (DHEA, 10.2 mg/kg) and 3) DHEA supplementation with WWBV training (DHEA: 10.2 mg/kg; WBV: 5.6 Hz, 2 mm, 0.13 g).
29725246	3	43	dep	DHEA	1010:1013	arg1	mm					1043:1044	5.6 Hz, 2 mm	1033:1044	mm	1043:1044	Methods: Male middle-aged C57BL/6 mice were divided into 3 groups (n = 8 per group) and treated for 4 weeks with the following: 1) Sedentary control (SC) with vehicle 2) DHEA supplementation (DHEA, 10.2 mg/kg) and 3) DHEA supplementation with WWBV training (DHEA: 10.2 mg/kg; WBV: 5.6 Hz, 2 mm, 0.13 g).
29725246	3	43	dep	DHEA	1010:1013	arg1	mg/kg					1021:1025	10.2 mg/kg	1016:1025	10.2 mg/kg	1016:1025	Methods: Male middle-aged C57BL/6 mice were divided into 3 groups (n = 8 per group) and treated for 4 weeks with the following: 1) Sedentary control (SC) with vehicle 2) DHEA supplementation (DHEA, 10.2 mg/kg) and 3) DHEA supplementation with WWBV training (DHEA: 10.2 mg/kg; WBV: 5.6 Hz, 2 mm, 0.13 g).
29725246	0	44	theme	Vibration	79:87	arg1	WWBV					99:102	WWBV	99:102	WWBV	99:102	Dehydroepiandrosterone supplementation combined with Weight-Loading Whole-Body Vibration Training (WWBV) affects exercise performance and muscle glycogen storage in middle-aged C57BL/6 mice.
29725246	0	44	theme	Vibration	79:87	arg1	Training					89:96	Weight-Loading Whole-Body Vibration Training	53:96	Weight-Loading Whole-Body Vibration Training (WWBV)	53:103	Dehydroepiandrosterone supplementation combined with Weight-Loading Whole-Body Vibration Training (WWBV) affects exercise performance and muscle glycogen storage in middle-aged C57BL/6 mice.
29725246	9	45	theme	15-min	1958:1963	arg1	test					1970:1973	the 15-min swim test	1954:1973	the 15-min swim test	1954:1973	WWBV+DHEA supplementation also decreased serum lactate, ammonia and BUN levels, while increasing glucose levels following the 15-min swim test.
29725246	5	46	theme	serum	1279:1283	arg1	lactate					1285:1291	serum lactate	1279:1291	serum lactate	1279:1291	Fatigue-related biochemical responses of serum lactate, ammonia, glucose, creatine kinase (CK), and blood urea nitrogen (BUN) were measured following the swimming exercise.
29725246	7	47	theme	WWBV	1567:1570	arg1	training					1572:1579	WWBV training	1567:1579	WWBV training	1567:1579	Results: DHEA supplementation combined with WWBV training for 4 weeks significantly decreased the amount of white adipose tissue and increased the food and water intake.
29725246	3	48	theme	Hz	1037:1038	arg1	mm					1043:1044	5.6 Hz, 2 mm	1033:1044	mm	1043:1044	Methods: Male middle-aged C57BL/6 mice were divided into 3 groups (n = 8 per group) and treated for 4 weeks with the following: 1) Sedentary control (SC) with vehicle 2) DHEA supplementation (DHEA, 10.2 mg/kg) and 3) DHEA supplementation with WWBV training (DHEA: 10.2 mg/kg; WBV: 5.6 Hz, 2 mm, 0.13 g).
29725246	1	49	theme	important	267:275	arg1	program					255:261	an optimal training program	235:261	an optimal training program	235:261	Background: Adequate nutritional intake and an optimal training program are important elements of any strategy to preserve or increase muscle mass and strength during aging.
29725246	1	49	theme	important	267:275	arg1	elements					277:284	important elements	267:284	important elements of any strategy to preserve or increase muscle mass and strength during aging	267:362	Background: Adequate nutritional intake and an optimal training program are important elements of any strategy to preserve or increase muscle mass and strength during aging.
29725246	1	49	theme	important	267:275	arg1	intake					224:229	Adequate nutritional intake	203:229	Adequate nutritional intake	203:229	Background: Adequate nutritional intake and an optimal training program are important elements of any strategy to preserve or increase muscle mass and strength during aging.
29725246	1	49	theme	important	267:275	arg1	Background					191:200	Background	191:200	Background: Adequate nutritional intake and an optimal training program are important elements of any strategy to preserve or increase muscle mass and strength during aging.	191:363	Background: Adequate nutritional intake and an optimal training program are important elements of any strategy to preserve or increase muscle mass and strength during aging.
29725246	3	50	theme	DHEA	922:925	arg1	supplementation					927:941	DHEA supplementation	922:941	vehicle 2) DHEA supplementation (DHEA, 10.2 mg/kg)	911:960	Methods: Male middle-aged C57BL/6 mice were divided into 3 groups (n = 8 per group) and treated for 4 weeks with the following: 1) Sedentary control (SC) with vehicle 2) DHEA supplementation (DHEA, 10.2 mg/kg) and 3) DHEA supplementation with WWBV training (DHEA: 10.2 mg/kg; WBV: 5.6 Hz, 2 mm, 0.13 g).
29725246	5	51	theme	Fatigue-related	1238:1252	arg1	responses					1266:1274	Fatigue-related biochemical responses	1238:1274	Fatigue-related biochemical responses of serum lactate, ammonia, glucose, creatine kinase (CK), and blood urea nitrogen (BUN)	1238:1362	Fatigue-related biochemical responses of serum lactate, ammonia, glucose, creatine kinase (CK), and blood urea nitrogen (BUN) were measured following the swimming exercise.
29725246	7	52	theme	adipose	1637:1643	arg1	tissue					1645:1650	white adipose tissue	1631:1650	white adipose tissue	1631:1650	Results: DHEA supplementation combined with WWBV training for 4 weeks significantly decreased the amount of white adipose tissue and increased the food and water intake.
29725246	10	53	theme	WWBV	2043:2046	arg1	program					2057:2063	the WWBV training program	2039:2063	the WWBV training program	2039:2063	Conclusion: Taken together, our results suggest that combining the WWBV training program with DHEA supplementation could provide an anti-fatigue pharmacological effect for elderly populations.
29725246	1	54	theme	strategy	293:300	arg1	program					255:261	an optimal training program	235:261	an optimal training program	235:261	Background: Adequate nutritional intake and an optimal training program are important elements of any strategy to preserve or increase muscle mass and strength during aging.
29725246	1	54	theme	strategy	293:300	arg1	elements					277:284	important elements	267:284	important elements of any strategy to preserve or increase muscle mass and strength during aging	267:362	Background: Adequate nutritional intake and an optimal training program are important elements of any strategy to preserve or increase muscle mass and strength during aging.
29725246	1	54	theme	strategy	293:300	arg1	intake					224:229	Adequate nutritional intake	203:229	Adequate nutritional intake	203:229	Background: Adequate nutritional intake and an optimal training program are important elements of any strategy to preserve or increase muscle mass and strength during aging.
29725246	1	54	theme	strategy	293:300	arg1	Background					191:200	Background	191:200	Background: Adequate nutritional intake and an optimal training program are important elements of any strategy to preserve or increase muscle mass and strength during aging.	191:363	Background: Adequate nutritional intake and an optimal training program are important elements of any strategy to preserve or increase muscle mass and strength during aging.
29725246	4	55	theme	body	1163:1166	arg1	composition					1168:1178	body composition	1163:1178	body composition	1163:1178	Exercise performance was evaluated by forelimb grip strength and time to exhaustion, as well as changes in body composition and anti-fatigue levels after a 15-min swimming exercise.
29725246	8	56	theme	exercise	1742:1749	arg1	performance					1751:1761	exercise performance	1742:1761	exercise performance	1742:1761	Additionally, WWBV+DHEA supplementation improved exercise performance, testosterone levels and glycogen contents of both liver and muscle.
29725246	5	57	theme	kinase	1321:1326	arg1	responses					1266:1274	Fatigue-related biochemical responses	1238:1274	Fatigue-related biochemical responses of serum lactate, ammonia, glucose, creatine kinase (CK), and blood urea nitrogen (BUN)	1238:1362	Fatigue-related biochemical responses of serum lactate, ammonia, glucose, creatine kinase (CK), and blood urea nitrogen (BUN) were measured following the swimming exercise.
29725246	3	58	dep	vehicle	911:917	arg1	2					919:919	2	919:919	2	919:919	Methods: Male middle-aged C57BL/6 mice were divided into 3 groups (n = 8 per group) and treated for 4 weeks with the following: 1) Sedentary control (SC) with vehicle 2) DHEA supplementation (DHEA, 10.2 mg/kg) and 3) DHEA supplementation with WWBV training (DHEA: 10.2 mg/kg; WBV: 5.6 Hz, 2 mm, 0.13 g).
29725246	3	58	dep	vehicle	911:917	arg1	supplementation					927:941	DHEA supplementation	922:941	vehicle 2) DHEA supplementation (DHEA, 10.2 mg/kg)	911:960	Methods: Male middle-aged C57BL/6 mice were divided into 3 groups (n = 8 per group) and treated for 4 weeks with the following: 1) Sedentary control (SC) with vehicle 2) DHEA supplementation (DHEA, 10.2 mg/kg) and 3) DHEA supplementation with WWBV training (DHEA: 10.2 mg/kg; WBV: 5.6 Hz, 2 mm, 0.13 g).
29725246	1	59	theme	nutritional	212:222	arg1	program					255:261	an optimal training program	235:261	an optimal training program	235:261	Background: Adequate nutritional intake and an optimal training program are important elements of any strategy to preserve or increase muscle mass and strength during aging.
29725246	1	59	theme	nutritional	212:222	arg1	intake					224:229	Adequate nutritional intake	203:229	Adequate nutritional intake	203:229	Background: Adequate nutritional intake and an optimal training program are important elements of any strategy to preserve or increase muscle mass and strength during aging.
29725246	1	59	theme	nutritional	212:222	arg1	elements					277:284	important elements	267:284	important elements of any strategy to preserve or increase muscle mass and strength during aging	267:362	Background: Adequate nutritional intake and an optimal training program are important elements of any strategy to preserve or increase muscle mass and strength during aging.
29725246	1	59	theme	nutritional	212:222	arg1	Background					191:200	Background	191:200	Background: Adequate nutritional intake and an optimal training program are important elements of any strategy to preserve or increase muscle mass and strength during aging.	191:363	Background: Adequate nutritional intake and an optimal training program are important elements of any strategy to preserve or increase muscle mass and strength during aging.
29725246	2	60	theme	exercise	616:623	arg1	performance					625:635	exercise performance	616:635	exercise performance	616:635	Purpose: In the current study, we investigate the effects of Dehydroepiandrosterone (DHEA), one of the most abundant circulating steroids in humans and a precursor hormone, supplementation combined with a weight-loading whole-body vibration (WWBV) on exercise performance, physical fatigue-related biochemical responses and testosterone content in middle-aged 9 months old C57BL/6 mice.
29725246	2	61	from	steroids	494:501	arg1	humans					506:511	humans	506:511	humans	506:511	Purpose: In the current study, we investigate the effects of Dehydroepiandrosterone (DHEA), one of the most abundant circulating steroids in humans and a precursor hormone, supplementation combined with a weight-loading whole-body vibration (WWBV) on exercise performance, physical fatigue-related biochemical responses and testosterone content in middle-aged 9 months old C57BL/6 mice.
29725246	3	62	theme	middle-aged	766:776	arg1	mice					786:789	Male middle-aged C57BL/6 mice	761:789	Male middle-aged C57BL/6 mice	761:789	Methods: Male middle-aged C57BL/6 mice were divided into 3 groups (n = 8 per group) and treated for 4 weeks with the following: 1) Sedentary control (SC) with vehicle 2) DHEA supplementation (DHEA, 10.2 mg/kg) and 3) DHEA supplementation with WWBV training (DHEA: 10.2 mg/kg; WBV: 5.6 Hz, 2 mm, 0.13 g).
29725246	0	63	theme	middle-aged	165:175	arg1	mice					185:188	middle-aged C57BL/6 mice	165:188	middle-aged C57BL/6 mice	165:188	Dehydroepiandrosterone supplementation combined with Weight-Loading Whole-Body Vibration Training (WWBV) affects exercise performance and muscle glycogen storage in middle-aged C57BL/6 mice.
29725246	3	64	dep	DHEA	944:947	arg1	mg/kg					955:959	10.2 mg/kg	950:959	10.2 mg/kg	950:959	Methods: Male middle-aged C57BL/6 mice were divided into 3 groups (n = 8 per group) and treated for 4 weeks with the following: 1) Sedentary control (SC) with vehicle 2) DHEA supplementation (DHEA, 10.2 mg/kg) and 3) DHEA supplementation with WWBV training (DHEA: 10.2 mg/kg; WBV: 5.6 Hz, 2 mm, 0.13 g).
29725246	1	65	theme	optimal	238:244	arg1	program					255:261	an optimal training program	235:261	an optimal training program	235:261	Background: Adequate nutritional intake and an optimal training program are important elements of any strategy to preserve or increase muscle mass and strength during aging.
29725246	1	65	theme	optimal	238:244	arg1	elements					277:284	important elements	267:284	important elements of any strategy to preserve or increase muscle mass and strength during aging	267:362	Background: Adequate nutritional intake and an optimal training program are important elements of any strategy to preserve or increase muscle mass and strength during aging.
29725246	1	65	theme	optimal	238:244	arg1	intake					224:229	Adequate nutritional intake	203:229	Adequate nutritional intake	203:229	Background: Adequate nutritional intake and an optimal training program are important elements of any strategy to preserve or increase muscle mass and strength during aging.
29725246	1	65	theme	optimal	238:244	arg1	Background					191:200	Background	191:200	Background: Adequate nutritional intake and an optimal training program are important elements of any strategy to preserve or increase muscle mass and strength during aging.	191:363	Background: Adequate nutritional intake and an optimal training program are important elements of any strategy to preserve or increase muscle mass and strength during aging.
29725246	7	66	theme	DHEA	1532:1535	arg1	supplementation					1537:1551	DHEA supplementation	1532:1551	DHEA supplementation combined with WWBV training for 4 weeks	1532:1591	Results: DHEA supplementation combined with WWBV training for 4 weeks significantly decreased the amount of white adipose tissue and increased the food and water intake.
29725246	2	67	theme	C57BL/6	738:744	arg1	mice					746:749	middle-aged 9 months old C57BL/6 mice	713:749	middle-aged 9 months old C57BL/6 mice	713:749	Purpose: In the current study, we investigate the effects of Dehydroepiandrosterone (DHEA), one of the most abundant circulating steroids in humans and a precursor hormone, supplementation combined with a weight-loading whole-body vibration (WWBV) on exercise performance, physical fatigue-related biochemical responses and testosterone content in middle-aged 9 months old C57BL/6 mice.
29725246	4	68	theme	swimming	1219:1226	arg1	exercise					1228:1235	a 15-min swimming exercise	1210:1235	a 15-min swimming exercise	1210:1235	Exercise performance was evaluated by forelimb grip strength and time to exhaustion, as well as changes in body composition and anti-fatigue levels after a 15-min swimming exercise.
29725246	6	69	theme	biochemical	1428:1438	arg1	parameters					1440:1449	the biochemical parameters	1424:1449	the biochemical parameters	1424:1449	In addition, the biochemical parameters and the testosterone levels were measured at the end of the experiment.
29725246	2	70	theme	whole-body	585:594	arg1	WWBV					607:610	WWBV	607:610	WWBV	607:610	Purpose: In the current study, we investigate the effects of Dehydroepiandrosterone (DHEA), one of the most abundant circulating steroids in humans and a precursor hormone, supplementation combined with a weight-loading whole-body vibration (WWBV) on exercise performance, physical fatigue-related biochemical responses and testosterone content in middle-aged 9 months old C57BL/6 mice.
29725246	2	70	theme	whole-body	585:594	arg1	vibration					596:604	a weight-loading whole-body vibration	568:604	a weight-loading whole-body vibration (WWBV)	568:611	Purpose: In the current study, we investigate the effects of Dehydroepiandrosterone (DHEA), one of the most abundant circulating steroids in humans and a precursor hormone, supplementation combined with a weight-loading whole-body vibration (WWBV) on exercise performance, physical fatigue-related biochemical responses and testosterone content in middle-aged 9 months old C57BL/6 mice.
29725246	6	71	theme	experiment	1511:1520	arg1	end					1500:1502	the end	1496:1502	the end of the experiment	1496:1520	In addition, the biochemical parameters and the testosterone levels were measured at the end of the experiment.
29725246	2	72	theme	months	727:732	arg1	mice					746:749	middle-aged 9 months old C57BL/6 mice	713:749	middle-aged 9 months old C57BL/6 mice	713:749	Purpose: In the current study, we investigate the effects of Dehydroepiandrosterone (DHEA), one of the most abundant circulating steroids in humans and a precursor hormone, supplementation combined with a weight-loading whole-body vibration (WWBV) on exercise performance, physical fatigue-related biochemical responses and testosterone content in middle-aged 9 months old C57BL/6 mice.
29725246	8	73	theme	liver	1814:1818	arg1	performance					1751:1761	exercise performance	1742:1761	exercise performance	1742:1761	Additionally, WWBV+DHEA supplementation improved exercise performance, testosterone levels and glycogen contents of both liver and muscle.
29725246	8	73	theme	liver	1814:1818	arg1	levels					1777:1782	testosterone levels	1764:1782	testosterone levels	1764:1782	Additionally, WWBV+DHEA supplementation improved exercise performance, testosterone levels and glycogen contents of both liver and muscle.
29725246	8	73	theme	liver	1814:1818	arg1	contents					1797:1804	glycogen contents	1788:1804	glycogen contents	1788:1804	Additionally, WWBV+DHEA supplementation improved exercise performance, testosterone levels and glycogen contents of both liver and muscle.
29725246	2	74	from	hormone	529:535	arg1	humans					506:511	humans	506:511	humans	506:511	Purpose: In the current study, we investigate the effects of Dehydroepiandrosterone (DHEA), one of the most abundant circulating steroids in humans and a precursor hormone, supplementation combined with a weight-loading whole-body vibration (WWBV) on exercise performance, physical fatigue-related biochemical responses and testosterone content in middle-aged 9 months old C57BL/6 mice.
29725246	2	75	from	responses	675:683	arg1	mice					746:749	middle-aged 9 months old C57BL/6 mice	713:749	middle-aged 9 months old C57BL/6 mice	713:749	Purpose: In the current study, we investigate the effects of Dehydroepiandrosterone (DHEA), one of the most abundant circulating steroids in humans and a precursor hormone, supplementation combined with a weight-loading whole-body vibration (WWBV) on exercise performance, physical fatigue-related biochemical responses and testosterone content in middle-aged 9 months old C57BL/6 mice.
29725246	3	76	theme	=	821:821	arg1	n					819:819	n = 8	819:823	n = 8	819:823	Methods: Male middle-aged C57BL/6 mice were divided into 3 groups (n = 8 per group) and treated for 4 weeks with the following: 1) Sedentary control (SC) with vehicle 2) DHEA supplementation (DHEA, 10.2 mg/kg) and 3) DHEA supplementation with WWBV training (DHEA: 10.2 mg/kg; WBV: 5.6 Hz, 2 mm, 0.13 g).
29725246	8	77	theme	muscle	1824:1829	arg1	performance					1751:1761	exercise performance	1742:1761	exercise performance	1742:1761	Additionally, WWBV+DHEA supplementation improved exercise performance, testosterone levels and glycogen contents of both liver and muscle.
29725246	8	77	theme	muscle	1824:1829	arg1	levels					1777:1782	testosterone levels	1764:1782	testosterone levels	1764:1782	Additionally, WWBV+DHEA supplementation improved exercise performance, testosterone levels and glycogen contents of both liver and muscle.
29725246	8	77	theme	muscle	1824:1829	arg1	contents					1797:1804	glycogen contents	1788:1804	glycogen contents	1788:1804	Additionally, WWBV+DHEA supplementation improved exercise performance, testosterone levels and glycogen contents of both liver and muscle.
29725246	3	78	with	supplementation	974:988	arg1	training					1000:1007	WWBV training	995:1007	WWBV training (DHEA: 10.2 mg/kg; WBV: 5.6 Hz, 2 mm, 0.13 g)	995:1053	Methods: Male middle-aged C57BL/6 mice were divided into 3 groups (n = 8 per group) and treated for 4 weeks with the following: 1) Sedentary control (SC) with vehicle 2) DHEA supplementation (DHEA, 10.2 mg/kg) and 3) DHEA supplementation with WWBV training (DHEA: 10.2 mg/kg; WBV: 5.6 Hz, 2 mm, 0.13 g).
29725246	10	79	theme	training	2048:2055	arg1	program					2057:2063	the WWBV training program	2039:2063	the WWBV training program	2039:2063	Conclusion: Taken together, our results suggest that combining the WWBV training program with DHEA supplementation could provide an anti-fatigue pharmacological effect for elderly populations.
29725246	3	80	theme	WWBV	995:998	arg1	training					1000:1007	WWBV training	995:1007	WWBV training (DHEA: 10.2 mg/kg; WBV: 5.6 Hz, 2 mm, 0.13 g)	995:1053	Methods: Male middle-aged C57BL/6 mice were divided into 3 groups (n = 8 per group) and treated for 4 weeks with the following: 1) Sedentary control (SC) with vehicle 2) DHEA supplementation (DHEA, 10.2 mg/kg) and 3) DHEA supplementation with WWBV training (DHEA: 10.2 mg/kg; WBV: 5.6 Hz, 2 mm, 0.13 g).
29725246	3	81	theme	following	869:877	arg1	SC					902:903	SC	902:903	SC	902:903	Methods: Male middle-aged C57BL/6 mice were divided into 3 groups (n = 8 per group) and treated for 4 weeks with the following: 1) Sedentary control (SC) with vehicle 2) DHEA supplementation (DHEA, 10.2 mg/kg) and 3) DHEA supplementation with WWBV training (DHEA: 10.2 mg/kg; WBV: 5.6 Hz, 2 mm, 0.13 g).
29725246	3	81	theme	following	869:877	arg1	control					893:899	the following: 1) Sedentary control	865:899	the following: 1) Sedentary control (SC)	865:904	Methods: Male middle-aged C57BL/6 mice were divided into 3 groups (n = 8 per group) and treated for 4 weeks with the following: 1) Sedentary control (SC) with vehicle 2) DHEA supplementation (DHEA, 10.2 mg/kg) and 3) DHEA supplementation with WWBV training (DHEA: 10.2 mg/kg; WBV: 5.6 Hz, 2 mm, 0.13 g).
29725246	2	82	theme	circulating	482:492	arg1	steroids					494:501	the most abundant circulating steroids	464:501	the most abundant circulating steroids in humans	464:511	Purpose: In the current study, we investigate the effects of Dehydroepiandrosterone (DHEA), one of the most abundant circulating steroids in humans and a precursor hormone, supplementation combined with a weight-loading whole-body vibration (WWBV) on exercise performance, physical fatigue-related biochemical responses and testosterone content in middle-aged 9 months old C57BL/6 mice.
29725246	5	83	theme	blood	1338:1342	arg1	BUN					1359:1361	BUN	1359:1361	BUN	1359:1361	Fatigue-related biochemical responses of serum lactate, ammonia, glucose, creatine kinase (CK), and blood urea nitrogen (BUN) were measured following the swimming exercise.
29725246	5	83	theme	blood	1338:1342	arg1	nitrogen					1349:1356	blood urea nitrogen	1338:1356	blood urea nitrogen (BUN)	1338:1362	Fatigue-related biochemical responses of serum lactate, ammonia, glucose, creatine kinase (CK), and blood urea nitrogen (BUN) were measured following the swimming exercise.
29725246	9	84	theme	lactate	1879:1885	arg1	levels					1904:1909	serum lactate, ammonia and BUN levels	1873:1909	serum lactate, ammonia and BUN levels	1873:1909	WWBV+DHEA supplementation also decreased serum lactate, ammonia and BUN levels, while increasing glucose levels following the 15-min swim test.
29725246	9	85	theme	ammonia	1888:1894	arg1	levels					1904:1909	serum lactate, ammonia and BUN levels	1873:1909	serum lactate, ammonia and BUN levels	1873:1909	WWBV+DHEA supplementation also decreased serum lactate, ammonia and BUN levels, while increasing glucose levels following the 15-min swim test.
29725246	4	86	from	time	1121:1124	arg1	composition					1168:1178	body composition	1163:1178	body composition	1163:1178	Exercise performance was evaluated by forelimb grip strength and time to exhaustion, as well as changes in body composition and anti-fatigue levels after a 15-min swimming exercise.
29725246	4	86	from	time	1121:1124	arg1	levels					1197:1202	anti-fatigue levels	1184:1202	anti-fatigue levels after a 15-min swimming exercise	1184:1235	Exercise performance was evaluated by forelimb grip strength and time to exhaustion, as well as changes in body composition and anti-fatigue levels after a 15-min swimming exercise.
29725246	2	87	theme	precursor	519:527	arg1	hormone					529:535	a precursor hormone	517:535	a precursor hormone	517:535	Purpose: In the current study, we investigate the effects of Dehydroepiandrosterone (DHEA), one of the most abundant circulating steroids in humans and a precursor hormone, supplementation combined with a weight-loading whole-body vibration (WWBV) on exercise performance, physical fatigue-related biochemical responses and testosterone content in middle-aged 9 months old C57BL/6 mice.
29725246	5	88	theme	nitrogen	1349:1356	arg1	responses					1266:1274	Fatigue-related biochemical responses	1238:1274	Fatigue-related biochemical responses of serum lactate, ammonia, glucose, creatine kinase (CK), and blood urea nitrogen (BUN)	1238:1362	Fatigue-related biochemical responses of serum lactate, ammonia, glucose, creatine kinase (CK), and blood urea nitrogen (BUN) were measured following the swimming exercise.
29725246	2	89	theme	biochemical	663:673	arg1	responses					675:683	physical fatigue-related biochemical responses	638:683	physical fatigue-related biochemical responses	638:683	Purpose: In the current study, we investigate the effects of Dehydroepiandrosterone (DHEA), one of the most abundant circulating steroids in humans and a precursor hormone, supplementation combined with a weight-loading whole-body vibration (WWBV) on exercise performance, physical fatigue-related biochemical responses and testosterone content in middle-aged 9 months old C57BL/6 mice.
29725246	0	90	theme	muscle	138:143	arg1	storage					154:160	muscle glycogen storage	138:160	muscle glycogen storage	138:160	Dehydroepiandrosterone supplementation combined with Weight-Loading Whole-Body Vibration Training (WWBV) affects exercise performance and muscle glycogen storage in middle-aged C57BL/6 mice.
29725246	4	91	from	strength	1108:1115	arg1	composition					1168:1178	body composition	1163:1178	body composition	1163:1178	Exercise performance was evaluated by forelimb grip strength and time to exhaustion, as well as changes in body composition and anti-fatigue levels after a 15-min swimming exercise.
29725246	4	91	from	strength	1108:1115	arg1	levels					1197:1202	anti-fatigue levels	1184:1202	anti-fatigue levels after a 15-min swimming exercise	1184:1235	Exercise performance was evaluated by forelimb grip strength and time to exhaustion, as well as changes in body composition and anti-fatigue levels after a 15-min swimming exercise.
29725246	3	92	theme	Sedentary	883:891	arg1	SC					902:903	SC	902:903	SC	902:903	Methods: Male middle-aged C57BL/6 mice were divided into 3 groups (n = 8 per group) and treated for 4 weeks with the following: 1) Sedentary control (SC) with vehicle 2) DHEA supplementation (DHEA, 10.2 mg/kg) and 3) DHEA supplementation with WWBV training (DHEA: 10.2 mg/kg; WBV: 5.6 Hz, 2 mm, 0.13 g).
29725246	3	92	theme	Sedentary	883:891	arg1	control					893:899	the following: 1) Sedentary control	865:899	the following: 1) Sedentary control (SC)	865:904	Methods: Male middle-aged C57BL/6 mice were divided into 3 groups (n = 8 per group) and treated for 4 weeks with the following: 1) Sedentary control (SC) with vehicle 2) DHEA supplementation (DHEA, 10.2 mg/kg) and 3) DHEA supplementation with WWBV training (DHEA: 10.2 mg/kg; WBV: 5.6 Hz, 2 mm, 0.13 g).
29725246	2	93	theme	middle-aged	713:723	arg1	mice					746:749	middle-aged 9 months old C57BL/6 mice	713:749	middle-aged 9 months old C57BL/6 mice	713:749	Purpose: In the current study, we investigate the effects of Dehydroepiandrosterone (DHEA), one of the most abundant circulating steroids in humans and a precursor hormone, supplementation combined with a weight-loading whole-body vibration (WWBV) on exercise performance, physical fatigue-related biochemical responses and testosterone content in middle-aged 9 months old C57BL/6 mice.
29725246	3	94	dep	supplementation	974:988	arg1	3					966:966	3	966:966	3	966:966	Methods: Male middle-aged C57BL/6 mice were divided into 3 groups (n = 8 per group) and treated for 4 weeks with the following: 1) Sedentary control (SC) with vehicle 2) DHEA supplementation (DHEA, 10.2 mg/kg) and 3) DHEA supplementation with WWBV training (DHEA: 10.2 mg/kg; WBV: 5.6 Hz, 2 mm, 0.13 g).
29725246	6	95	theme	testosterone	1459:1470	arg1	levels					1472:1477	the testosterone levels	1455:1477	the testosterone levels	1455:1477	In addition, the biochemical parameters and the testosterone levels were measured at the end of the experiment.
29725246	8	96	theme	glycogen	1788:1795	arg1	contents					1797:1804	glycogen contents	1788:1804	glycogen contents	1788:1804	Additionally, WWBV+DHEA supplementation improved exercise performance, testosterone levels and glycogen contents of both liver and muscle.
29725246	4	97	theme	forelimb	1094:1101	arg1	strength					1108:1115	forelimb grip strength	1094:1115	forelimb grip strength	1094:1115	Exercise performance was evaluated by forelimb grip strength and time to exhaustion, as well as changes in body composition and anti-fatigue levels after a 15-min swimming exercise.
29725246	10	98	theme	DHEA	2070:2073	arg1	supplementation					2075:2089	DHEA supplementation	2070:2089	DHEA supplementation	2070:2089	Conclusion: Taken together, our results suggest that combining the WWBV training program with DHEA supplementation could provide an anti-fatigue pharmacological effect for elderly populations.
29725246	7	99	theme	food	1670:1673	arg1	intake					1685:1690	the food and water intake	1666:1690	intake	1685:1690	Results: DHEA supplementation combined with WWBV training for 4 weeks significantly decreased the amount of white adipose tissue and increased the food and water intake.
29725246	4	100	from	changes	1152:1158	arg1	composition					1168:1178	body composition	1163:1178	body composition	1163:1178	Exercise performance was evaluated by forelimb grip strength and time to exhaustion, as well as changes in body composition and anti-fatigue levels after a 15-min swimming exercise.
29725246	4	100	from	changes	1152:1158	arg1	levels					1197:1202	anti-fatigue levels	1184:1202	anti-fatigue levels after a 15-min swimming exercise	1184:1235	Exercise performance was evaluated by forelimb grip strength and time to exhaustion, as well as changes in body composition and anti-fatigue levels after a 15-min swimming exercise.
29725246	2	101	theme	Dehydroepiandrosterone	426:447	arg1	effects					415:421	the effects	411:421	the effects of Dehydroepiandrosterone (DHEA), one of the most abundant circulating steroids in humans and a precursor hormone, supplementation combined with a weight-loading whole-body vibration (WWBV) on exercise performance, physical fatigue-related biochemical responses and testosterone content in middle-aged 9 months old C57BL/6 mice	411:749	Purpose: In the current study, we investigate the effects of Dehydroepiandrosterone (DHEA), one of the most abundant circulating steroids in humans and a precursor hormone, supplementation combined with a weight-loading whole-body vibration (WWBV) on exercise performance, physical fatigue-related biochemical responses and testosterone content in middle-aged 9 months old C57BL/6 mice.
29725246	0	102	theme	Whole-Body	68:77	arg1	WWBV					99:102	WWBV	99:102	WWBV	99:102	Dehydroepiandrosterone supplementation combined with Weight-Loading Whole-Body Vibration Training (WWBV) affects exercise performance and muscle glycogen storage in middle-aged C57BL/6 mice.
29725246	0	102	theme	Whole-Body	68:77	arg1	Training					89:96	Weight-Loading Whole-Body Vibration Training	53:96	Weight-Loading Whole-Body Vibration Training (WWBV)	53:103	Dehydroepiandrosterone supplementation combined with Weight-Loading Whole-Body Vibration Training (WWBV) affects exercise performance and muscle glycogen storage in middle-aged C57BL/6 mice.
29725246	1	103	theme	muscle	326:331	arg1	mass					333:336	muscle mass	326:336	muscle mass	326:336	Background: Adequate nutritional intake and an optimal training program are important elements of any strategy to preserve or increase muscle mass and strength during aging.
29725246	2	104	from	performance	625:635	arg1	mice					746:749	middle-aged 9 months old C57BL/6 mice	713:749	middle-aged 9 months old C57BL/6 mice	713:749	Purpose: In the current study, we investigate the effects of Dehydroepiandrosterone (DHEA), one of the most abundant circulating steroids in humans and a precursor hormone, supplementation combined with a weight-loading whole-body vibration (WWBV) on exercise performance, physical fatigue-related biochemical responses and testosterone content in middle-aged 9 months old C57BL/6 mice.
29725246	5	105	theme	swimming	1392:1399	arg1	exercise					1401:1408	the swimming exercise	1388:1408	the swimming exercise	1388:1408	Fatigue-related biochemical responses of serum lactate, ammonia, glucose, creatine kinase (CK), and blood urea nitrogen (BUN) were measured following the swimming exercise.
29725246	5	106	theme	biochemical	1254:1264	arg1	responses					1266:1274	Fatigue-related biochemical responses	1238:1274	Fatigue-related biochemical responses of serum lactate, ammonia, glucose, creatine kinase (CK), and blood urea nitrogen (BUN)	1238:1362	Fatigue-related biochemical responses of serum lactate, ammonia, glucose, creatine kinase (CK), and blood urea nitrogen (BUN) were measured following the swimming exercise.
29725246	9	107	theme	BUN	1900:1902	arg1	levels					1904:1909	serum lactate, ammonia and BUN levels	1873:1909	serum lactate, ammonia and BUN levels	1873:1909	WWBV+DHEA supplementation also decreased serum lactate, ammonia and BUN levels, while increasing glucose levels following the 15-min swim test.
29725246	7	108	theme	water	1679:1683	arg1	intake					1685:1690	the food and water intake	1666:1690	intake	1685:1690	Results: DHEA supplementation combined with WWBV training for 4 weeks significantly decreased the amount of white adipose tissue and increased the food and water intake.
29725246	9	109	theme	swim	1965:1968	arg1	test					1970:1973	the 15-min swim test	1954:1973	the 15-min swim test	1954:1973	WWBV+DHEA supplementation also decreased serum lactate, ammonia and BUN levels, while increasing glucose levels following the 15-min swim test.
29725246	2	110	from	effects	415:421	arg1	responses					675:683	physical fatigue-related biochemical responses	638:683	physical fatigue-related biochemical responses	638:683	Purpose: In the current study, we investigate the effects of Dehydroepiandrosterone (DHEA), one of the most abundant circulating steroids in humans and a precursor hormone, supplementation combined with a weight-loading whole-body vibration (WWBV) on exercise performance, physical fatigue-related biochemical responses and testosterone content in middle-aged 9 months old C57BL/6 mice.
29725246	2	110	from	effects	415:421	arg1	content					702:708	testosterone content	689:708	testosterone content	689:708	Purpose: In the current study, we investigate the effects of Dehydroepiandrosterone (DHEA), one of the most abundant circulating steroids in humans and a precursor hormone, supplementation combined with a weight-loading whole-body vibration (WWBV) on exercise performance, physical fatigue-related biochemical responses and testosterone content in middle-aged 9 months old C57BL/6 mice.
29725246	2	110	from	effects	415:421	arg1	performance					625:635	exercise performance	616:635	exercise performance	616:635	Purpose: In the current study, we investigate the effects of Dehydroepiandrosterone (DHEA), one of the most abundant circulating steroids in humans and a precursor hormone, supplementation combined with a weight-loading whole-body vibration (WWBV) on exercise performance, physical fatigue-related biochemical responses and testosterone content in middle-aged 9 months old C57BL/6 mice.
29725246	0	111	from	storage	154:160	arg1	mice					185:188	middle-aged C57BL/6 mice	165:188	middle-aged C57BL/6 mice	165:188	Dehydroepiandrosterone supplementation combined with Weight-Loading Whole-Body Vibration Training (WWBV) affects exercise performance and muscle glycogen storage in middle-aged C57BL/6 mice.
29725246	2	112	theme	current	381:387	arg1	study					389:393	the current study	377:393	the current study	377:393	Purpose: In the current study, we investigate the effects of Dehydroepiandrosterone (DHEA), one of the most abundant circulating steroids in humans and a precursor hormone, supplementation combined with a weight-loading whole-body vibration (WWBV) on exercise performance, physical fatigue-related biochemical responses and testosterone content in middle-aged 9 months old C57BL/6 mice.
31680219	6	0	theme	KGMB	925:928	arg1	03357T					930:935	strain KGMB 03357T	918:935	strain KGMB 03357T	918:935	The ANI values between strain KGMB 03357T and members of the genus Anaerotignum were 73.3-71.0%, which are below the ANI criterion for interspecies identity.
31680219	5	1	theme	genus	603:607	arg1	Anaerotignum					609:620	the genus Anaerotignum	599:620	the genus Anaerotignum	599:620	Analysis of phylogenetic trees based on the 16S rRNA gene sequences revealed that strain KGMB03357T forms a lineage within the genus Anaerotignum, and is most closely related to Anaerotignum lactatifermentans G17T (= KCTC 15066T, 96.1%), Anaerotignum propionicum DSM 1682T (= KCTC 5582T, 94.9%), Anaerotignum neopropionicum DSM 03847T (= KCTC 15564T, 94.9%), and Anaerotignum aminivorans SH021T (= KCTC 15705T, 94.8%).
31680219	10	2	theme	phenotypic	1352:1361	arg1	properties					1390:1399	the phenotypic, phylogenetic, and genomic properties	1348:1399	the phenotypic, phylogenetic, and genomic properties	1348:1399	Based on the phenotypic, phylogenetic, and genomic properties, strain KGMB 03357T represents a novel species of the genus Anaerotignum, for which the name Anaerotignum faecicola sp.
31680219	7	3	theme	DNA	1057:1059	arg1	%					1121:1121	47.3 mol%	1113:1121	47.3 mol%	1113:1121	The DNA G + C content based on the whole-genome sequence is 47.3 mol%.
31680219	7	3	theme	DNA	1057:1059	arg1	G					1061:1061	The DNA G + C content	1053:1073	G	1061:1061	The DNA G + C content based on the whole-genome sequence is 47.3 mol%.
31680219	6	4	theme	ANI	899:901	arg1	%					989:989	73.3-71.0%	980:989	73.3-71.0%	980:989	The ANI values between strain KGMB 03357T and members of the genus Anaerotignum were 73.3-71.0%, which are below the ANI criterion for interspecies identity.
31680219	6	4	theme	ANI	899:901	arg1	values					903:908	The ANI values	895:908	The ANI values between strain KGMB 03357T and members of the genus Anaerotignum	895:973	The ANI values between strain KGMB 03357T and members of the genus Anaerotignum were 73.3-71.0%, which are below the ANI criterion for interspecies identity.
31680219	5	5	dep	15705T	879:884	arg1	%					891:891	94.8%	887:891	94.8%	887:891	Analysis of phylogenetic trees based on the 16S rRNA gene sequences revealed that strain KGMB03357T forms a lineage within the genus Anaerotignum, and is most closely related to Anaerotignum lactatifermentans G17T (= KCTC 15066T, 96.1%), Anaerotignum propionicum DSM 1682T (= KCTC 5582T, 94.9%), Anaerotignum neopropionicum DSM 03847T (= KCTC 15564T, 94.9%), and Anaerotignum aminivorans SH021T (= KCTC 15705T, 94.8%).
31680219	5	6	theme	strain	558:563	arg1	KGMB03357T					565:574	strain KGMB03357T	558:574	strain KGMB03357T	558:574	Analysis of phylogenetic trees based on the 16S rRNA gene sequences revealed that strain KGMB03357T forms a lineage within the genus Anaerotignum, and is most closely related to Anaerotignum lactatifermentans G17T (= KCTC 15066T, 96.1%), Anaerotignum propionicum DSM 1682T (= KCTC 5582T, 94.9%), Anaerotignum neopropionicum DSM 03847T (= KCTC 15564T, 94.9%), and Anaerotignum aminivorans SH021T (= KCTC 15705T, 94.8%).
31680219	0	7	theme	Anaerotignum	0:11	arg1	sp					23:24	Anaerotignum faecicola sp	0:24	Anaerotignum faecicola sp.	0:25	Anaerotignum faecicola sp.
31680219	9	8	theme	Strain	1226:1231	arg1	KGMB03357T					1233:1242	Strain KGMB03357T	1226:1242	Strain KGMB03357T	1226:1242	Strain KGMB03357T contains meso-diaminopimelic acid as the diagnostic amino acid in the cell wall peptidoglycan.
31680219	5	9	theme	=	812:812	arg1	15564T					819:824	= KCTC 15564T	812:824	= KCTC 15564T	812:824	Analysis of phylogenetic trees based on the 16S rRNA gene sequences revealed that strain KGMB03357T forms a lineage within the genus Anaerotignum, and is most closely related to Anaerotignum lactatifermentans G17T (= KCTC 15066T, 96.1%), Anaerotignum propionicum DSM 1682T (= KCTC 5582T, 94.9%), Anaerotignum neopropionicum DSM 03847T (= KCTC 15564T, 94.9%), and Anaerotignum aminivorans SH021T (= KCTC 15705T, 94.8%).
31680219	5	9	theme	=	812:812	arg1	%					831:831	94.9%	827:831	94.9%	827:831	Analysis of phylogenetic trees based on the 16S rRNA gene sequences revealed that strain KGMB03357T forms a lineage within the genus Anaerotignum, and is most closely related to Anaerotignum lactatifermentans G17T (= KCTC 15066T, 96.1%), Anaerotignum propionicum DSM 1682T (= KCTC 5582T, 94.9%), Anaerotignum neopropionicum DSM 03847T (= KCTC 15564T, 94.9%), and Anaerotignum aminivorans SH021T (= KCTC 15705T, 94.8%).
31680219	10	10	theme	phylogenetic	1364:1375	arg1	properties					1390:1399	the phenotypic, phylogenetic, and genomic properties	1348:1399	the phenotypic, phylogenetic, and genomic properties	1348:1399	Based on the phenotypic, phylogenetic, and genomic properties, strain KGMB 03357T represents a novel species of the genus Anaerotignum, for which the name Anaerotignum faecicola sp.
31680219	5	11	dep	Anaerotignum	839:850	arg1	aminivorans					852:862	aminivorans	852:862	aminivorans	852:862	Analysis of phylogenetic trees based on the 16S rRNA gene sequences revealed that strain KGMB03357T forms a lineage within the genus Anaerotignum, and is most closely related to Anaerotignum lactatifermentans G17T (= KCTC 15066T, 96.1%), Anaerotignum propionicum DSM 1682T (= KCTC 5582T, 94.9%), Anaerotignum neopropionicum DSM 03847T (= KCTC 15564T, 94.9%), and Anaerotignum aminivorans SH021T (= KCTC 15705T, 94.8%).
31680219	10	12	theme	strain	1402:1407	arg1	03357T					1414:1419	strain KGMB 03357T	1402:1419	strain KGMB 03357T	1402:1419	Based on the phenotypic, phylogenetic, and genomic properties, strain KGMB 03357T represents a novel species of the genus Anaerotignum, for which the name Anaerotignum faecicola sp.
31680219	5	13	theme	KCTC	752:755	arg1	5582T					757:761	= KCTC 5582T	750:761	= KCTC 5582T	750:761	Analysis of phylogenetic trees based on the 16S rRNA gene sequences revealed that strain KGMB03357T forms a lineage within the genus Anaerotignum, and is most closely related to Anaerotignum lactatifermentans G17T (= KCTC 15066T, 96.1%), Anaerotignum propionicum DSM 1682T (= KCTC 5582T, 94.9%), Anaerotignum neopropionicum DSM 03847T (= KCTC 15564T, 94.9%), and Anaerotignum aminivorans SH021T (= KCTC 15705T, 94.8%).
31680219	5	13	theme	KCTC	752:755	arg1	%					768:768	94.9%	764:768	94.9%	764:768	Analysis of phylogenetic trees based on the 16S rRNA gene sequences revealed that strain KGMB03357T forms a lineage within the genus Anaerotignum, and is most closely related to Anaerotignum lactatifermentans G17T (= KCTC 15066T, 96.1%), Anaerotignum propionicum DSM 1682T (= KCTC 5582T, 94.9%), Anaerotignum neopropionicum DSM 03847T (= KCTC 15564T, 94.9%), and Anaerotignum aminivorans SH021T (= KCTC 15705T, 94.8%).
31680219	2	14	theme	anaerobic	72:80	arg1	bacterium					82:90	A strictly anaerobic bacterium	61:90	A strictly anaerobic bacterium	61:90	A strictly anaerobic bacterium, designated as strain KGMB-03357T, was isolated from the faeces of a healthy Korean selected by Bundang Seoul National University based on health status.
31680219	5	15	theme	phylogenetic	488:499	arg1	trees					501:505	phylogenetic trees	488:505	phylogenetic trees based on the 16S rRNA gene sequences	488:542	Analysis of phylogenetic trees based on the 16S rRNA gene sequences revealed that strain KGMB03357T forms a lineage within the genus Anaerotignum, and is most closely related to Anaerotignum lactatifermentans G17T (= KCTC 15066T, 96.1%), Anaerotignum propionicum DSM 1682T (= KCTC 5582T, 94.9%), Anaerotignum neopropionicum DSM 03847T (= KCTC 15564T, 94.9%), and Anaerotignum aminivorans SH021T (= KCTC 15705T, 94.8%).
31680219	3	16	theme	KGMB03357T	262:271	arg1	rods					364:367	straight or curved rods	345:367	straight or curved rods	345:367	Cells of strain KGMB03357T are Gram-stain-positive, non-motile, non-spore-forming, and observed as straight or curved rods.
31680219	3	16	theme	KGMB03357T	262:271	arg1	Cells					246:250	Cells	246:250	Cells of strain KGMB03357T	246:271	Cells of strain KGMB03357T are Gram-stain-positive, non-motile, non-spore-forming, and observed as straight or curved rods.
31680219	2	17	theme	health	231:236	arg1	status					238:243	health status	231:243	health status	231:243	A strictly anaerobic bacterium, designated as strain KGMB-03357T, was isolated from the faeces of a healthy Korean selected by Bundang Seoul National University based on health status.
31680219	8	18	dep	C18∶1	1194:1198	arg1	cis					1200:1202	cis 9	1200:1204	C18:0, C18∶1 cis 9	1187:1204	The major cellular fatty acids of strain KGMB03357T are C16:0, C18:0, C18∶1 cis 9, and anteiso-C15∶0.
31680219	4	19	theme	optimum	399:405	arg1	temperature					407:417	optimum temperature	399:417	optimum temperature of 40°C	399:425	The isolate grew at 10-45°C (optimum temperature of 40°C) and a pH range of 5.1-10.5 (optimum pH of 6.8).
31680219	6	20	theme	ANI	1012:1014	arg1	criterion					1016:1024	the ANI criterion	1008:1024	the ANI criterion for interspecies identity	1008:1050	The ANI values between strain KGMB 03357T and members of the genus Anaerotignum were 73.3-71.0%, which are below the ANI criterion for interspecies identity.
31680219	5	21	theme	=	691:691	arg1	G17T					685:688	Anaerotignum lactatifermentans G17T	654:688	Anaerotignum lactatifermentans G17T (= KCTC 15066T, 96.1%)	654:711	Analysis of phylogenetic trees based on the 16S rRNA gene sequences revealed that strain KGMB03357T forms a lineage within the genus Anaerotignum, and is most closely related to Anaerotignum lactatifermentans G17T (= KCTC 15066T, 96.1%), Anaerotignum propionicum DSM 1682T (= KCTC 5582T, 94.9%), Anaerotignum neopropionicum DSM 03847T (= KCTC 15564T, 94.9%), and Anaerotignum aminivorans SH021T (= KCTC 15705T, 94.8%).
31680219	5	21	theme	=	691:691	arg1	15066T					698:703	= KCTC 15066T	691:703	= KCTC 15066T	691:703	Analysis of phylogenetic trees based on the 16S rRNA gene sequences revealed that strain KGMB03357T forms a lineage within the genus Anaerotignum, and is most closely related to Anaerotignum lactatifermentans G17T (= KCTC 15066T, 96.1%), Anaerotignum propionicum DSM 1682T (= KCTC 5582T, 94.9%), Anaerotignum neopropionicum DSM 03847T (= KCTC 15564T, 94.9%), and Anaerotignum aminivorans SH021T (= KCTC 15705T, 94.8%).
31680219	4	22	theme	5.1-10.5	446:453	arg1	range					437:441	a pH range	432:441	a pH range of 5.1-10.5 (optimum pH of 6.8)	432:473	The isolate grew at 10-45°C (optimum temperature of 40°C) and a pH range of 5.1-10.5 (optimum pH of 6.8).
31680219	4	22	theme	5.1-10.5	446:453	arg1	10-45°C					390:396	10-45°C	390:396	10-45°C (optimum temperature of 40°C)	390:426	The isolate grew at 10-45°C (optimum temperature of 40°C) and a pH range of 5.1-10.5 (optimum pH of 6.8).
31680219	5	23	theme	rRNA	524:527	arg1	sequences					534:542	the 16S rRNA gene sequences	516:542	the 16S rRNA gene sequences	516:542	Analysis of phylogenetic trees based on the 16S rRNA gene sequences revealed that strain KGMB03357T forms a lineage within the genus Anaerotignum, and is most closely related to Anaerotignum lactatifermentans G17T (= KCTC 15066T, 96.1%), Anaerotignum propionicum DSM 1682T (= KCTC 5582T, 94.9%), Anaerotignum neopropionicum DSM 03847T (= KCTC 15564T, 94.9%), and Anaerotignum aminivorans SH021T (= KCTC 15705T, 94.8%).
31680219	9	24	theme	cell	1314:1317	arg1	peptidoglycan					1324:1336	the cell wall peptidoglycan	1310:1336	the cell wall peptidoglycan	1310:1336	Strain KGMB03357T contains meso-diaminopimelic acid as the diagnostic amino acid in the cell wall peptidoglycan.
31680219	4	25	theme	optimum	456:462	arg1	pH					464:465	optimum pH	456:465	optimum pH of 6.8	456:472	The isolate grew at 10-45°C (optimum temperature of 40°C) and a pH range of 5.1-10.5 (optimum pH of 6.8).
31680219	12	26	theme	type	1543:1546	arg1	KGMB03357T					1558:1567	KGMB03357T	1558:1567	KGMB03357T (= KCTC 15736T = DSM 107953T)	1558:1597	The type strain is KGMB03357T (= KCTC 15736T = DSM 107953T).
31680219	12	26	theme	type	1543:1546	arg1	strain					1548:1553	The type strain	1539:1553	The type strain	1539:1553	The type strain is KGMB03357T (= KCTC 15736T = DSM 107953T).
31680219	8	27	theme	cellular	1134:1141	arg1	C16:0					1180:1184	C16:0	1180:1184	C16:0	1180:1184	The major cellular fatty acids of strain KGMB03357T are C16:0, C18:0, C18∶1 cis 9, and anteiso-C15∶0.
31680219	8	27	theme	cellular	1134:1141	arg1	acids					1149:1153	The major cellular fatty acids	1124:1153	The major cellular fatty acids of strain KGMB03357T	1124:1174	The major cellular fatty acids of strain KGMB03357T are C16:0, C18:0, C18∶1 cis 9, and anteiso-C15∶0.
31680219	5	28	theme	Anaerotignum	654:665	arg1	G17T					685:688	Anaerotignum lactatifermentans G17T	654:688	Anaerotignum lactatifermentans G17T (= KCTC 15066T, 96.1%)	654:711	Analysis of phylogenetic trees based on the 16S rRNA gene sequences revealed that strain KGMB03357T forms a lineage within the genus Anaerotignum, and is most closely related to Anaerotignum lactatifermentans G17T (= KCTC 15066T, 96.1%), Anaerotignum propionicum DSM 1682T (= KCTC 5582T, 94.9%), Anaerotignum neopropionicum DSM 03847T (= KCTC 15564T, 94.9%), and Anaerotignum aminivorans SH021T (= KCTC 15705T, 94.8%).
31680219	5	28	theme	Anaerotignum	654:665	arg1	15066T					698:703	= KCTC 15066T	691:703	= KCTC 15066T	691:703	Analysis of phylogenetic trees based on the 16S rRNA gene sequences revealed that strain KGMB03357T forms a lineage within the genus Anaerotignum, and is most closely related to Anaerotignum lactatifermentans G17T (= KCTC 15066T, 96.1%), Anaerotignum propionicum DSM 1682T (= KCTC 5582T, 94.9%), Anaerotignum neopropionicum DSM 03847T (= KCTC 15564T, 94.9%), and Anaerotignum aminivorans SH021T (= KCTC 15705T, 94.8%).
31680219	9	29	theme	amino	1296:1300	arg1	acid					1302:1305	the diagnostic amino acid	1281:1305	the diagnostic amino acid in the cell wall peptidoglycan	1281:1336	Strain KGMB03357T contains meso-diaminopimelic acid as the diagnostic amino acid in the cell wall peptidoglycan.
31680219	9	29	theme	amino	1296:1300	arg1	acid					1273:1276	meso-diaminopimelic acid	1253:1276	meso-diaminopimelic acid	1253:1276	Strain KGMB03357T contains meso-diaminopimelic acid as the diagnostic amino acid in the cell wall peptidoglycan.
31680219	10	30	theme	novel	1434:1438	arg1	species					1440:1446	a novel species	1432:1446	a novel species	1432:1446	Based on the phenotypic, phylogenetic, and genomic properties, strain KGMB 03357T represents a novel species of the genus Anaerotignum, for which the name Anaerotignum faecicola sp.
31680219	9	31	contain	contains	1244:1251	arg2	acid					1273:1276	meso-diaminopimelic acid	1253:1276	meso-diaminopimelic acid	1253:1276	Strain KGMB03357T contains meso-diaminopimelic acid as the diagnostic amino acid in the cell wall peptidoglycan.
31680219	9	31	contain	contains	1244:1251	arg2	acid					1302:1305	the diagnostic amino acid	1281:1305	the diagnostic amino acid in the cell wall peptidoglycan	1281:1336	Strain KGMB03357T contains meso-diaminopimelic acid as the diagnostic amino acid in the cell wall peptidoglycan.
31680219	9	31	contain	contains	1244:1251	arg1	KGMB03357T					1233:1242	Strain KGMB03357T	1226:1242	Strain KGMB03357T	1226:1242	Strain KGMB03357T contains meso-diaminopimelic acid as the diagnostic amino acid in the cell wall peptidoglycan.
31680219	5	32	dep	Anaerotignum	772:783	arg1	neopropionicum					785:798	neopropionicum	785:798	neopropionicum	785:798	Analysis of phylogenetic trees based on the 16S rRNA gene sequences revealed that strain KGMB03357T forms a lineage within the genus Anaerotignum, and is most closely related to Anaerotignum lactatifermentans G17T (= KCTC 15066T, 96.1%), Anaerotignum propionicum DSM 1682T (= KCTC 5582T, 94.9%), Anaerotignum neopropionicum DSM 03847T (= KCTC 15564T, 94.9%), and Anaerotignum aminivorans SH021T (= KCTC 15705T, 94.8%).
31680219	5	33	theme	Anaerotignum	714:725	arg1	1682T					743:747	Anaerotignum propionicum DSM 1682T	714:747	Anaerotignum propionicum DSM 1682T (= KCTC 5582T, 94.9%)	714:769	Analysis of phylogenetic trees based on the 16S rRNA gene sequences revealed that strain KGMB03357T forms a lineage within the genus Anaerotignum, and is most closely related to Anaerotignum lactatifermentans G17T (= KCTC 15066T, 96.1%), Anaerotignum propionicum DSM 1682T (= KCTC 5582T, 94.9%), Anaerotignum neopropionicum DSM 03847T (= KCTC 15564T, 94.9%), and Anaerotignum aminivorans SH021T (= KCTC 15705T, 94.8%).
31680219	10	34	theme	genus	1455:1459	arg1	Anaerotignum					1461:1472	the genus Anaerotignum	1451:1472	the genus Anaerotignum	1451:1472	Based on the phenotypic, phylogenetic, and genomic properties, strain KGMB 03357T represents a novel species of the genus Anaerotignum, for which the name Anaerotignum faecicola sp.
31680219	5	35	theme	DSM	739:741	arg1	1682T					743:747	Anaerotignum propionicum DSM 1682T	714:747	Anaerotignum propionicum DSM 1682T (= KCTC 5582T, 94.9%)	714:769	Analysis of phylogenetic trees based on the 16S rRNA gene sequences revealed that strain KGMB03357T forms a lineage within the genus Anaerotignum, and is most closely related to Anaerotignum lactatifermentans G17T (= KCTC 15066T, 96.1%), Anaerotignum propionicum DSM 1682T (= KCTC 5582T, 94.9%), Anaerotignum neopropionicum DSM 03847T (= KCTC 15564T, 94.9%), and Anaerotignum aminivorans SH021T (= KCTC 15705T, 94.8%).
31680219	5	36	theme	KCTC	874:877	arg1	SH021T					864:869	Anaerotignum aminivorans SH021T	839:869	Anaerotignum aminivorans SH021T (= KCTC 15705T, 94.8%)	839:892	Analysis of phylogenetic trees based on the 16S rRNA gene sequences revealed that strain KGMB03357T forms a lineage within the genus Anaerotignum, and is most closely related to Anaerotignum lactatifermentans G17T (= KCTC 15066T, 96.1%), Anaerotignum propionicum DSM 1682T (= KCTC 5582T, 94.9%), Anaerotignum neopropionicum DSM 03847T (= KCTC 15564T, 94.9%), and Anaerotignum aminivorans SH021T (= KCTC 15705T, 94.8%).
31680219	5	36	theme	KCTC	874:877	arg1	15705T					879:884	= KCTC 15705T	872:884	= KCTC 15705T	872:884	Analysis of phylogenetic trees based on the 16S rRNA gene sequences revealed that strain KGMB03357T forms a lineage within the genus Anaerotignum, and is most closely related to Anaerotignum lactatifermentans G17T (= KCTC 15066T, 96.1%), Anaerotignum propionicum DSM 1682T (= KCTC 5582T, 94.9%), Anaerotignum neopropionicum DSM 03847T (= KCTC 15564T, 94.9%), and Anaerotignum aminivorans SH021T (= KCTC 15705T, 94.8%).
31680219	12	37	theme	KCTC	1572:1575	arg1	KGMB03357T					1558:1567	KGMB03357T	1558:1567	KGMB03357T (= KCTC 15736T = DSM 107953T)	1558:1597	The type strain is KGMB03357T (= KCTC 15736T = DSM 107953T).
31680219	12	37	theme	KCTC	1572:1575	arg1	107953T					1590:1596	= KCTC 15736T = DSM 107953T	1570:1596	= KCTC 15736T = DSM 107953T	1570:1596	The type strain is KGMB03357T (= KCTC 15736T = DSM 107953T).
31680219	12	38	theme	DSM	1586:1588	arg1	KGMB03357T					1558:1567	KGMB03357T	1558:1567	KGMB03357T (= KCTC 15736T = DSM 107953T)	1558:1597	The type strain is KGMB03357T (= KCTC 15736T = DSM 107953T).
31680219	12	38	theme	DSM	1586:1588	arg1	107953T					1590:1596	= KCTC 15736T = DSM 107953T	1570:1596	= KCTC 15736T = DSM 107953T	1570:1596	The type strain is KGMB03357T (= KCTC 15736T = DSM 107953T).
31680219	12	39	theme	=	1584:1584	arg1	KGMB03357T					1558:1567	KGMB03357T	1558:1567	KGMB03357T (= KCTC 15736T = DSM 107953T)	1558:1597	The type strain is KGMB03357T (= KCTC 15736T = DSM 107953T).
31680219	12	39	theme	=	1584:1584	arg1	107953T					1590:1596	= KCTC 15736T = DSM 107953T	1570:1596	= KCTC 15736T = DSM 107953T	1570:1596	The type strain is KGMB03357T (= KCTC 15736T = DSM 107953T).
31680219	2	40	theme	healthy	161:167	arg1	Korean					169:174	a healthy Korean	159:174	a healthy Korean selected by Bundang Seoul National University based on health status	159:243	A strictly anaerobic bacterium, designated as strain KGMB-03357T, was isolated from the faeces of a healthy Korean selected by Bundang Seoul National University based on health status.
31680219	5	41	dep	1682T	743:747	arg1	5582T					757:761	= KCTC 5582T	750:761	= KCTC 5582T	750:761	Analysis of phylogenetic trees based on the 16S rRNA gene sequences revealed that strain KGMB03357T forms a lineage within the genus Anaerotignum, and is most closely related to Anaerotignum lactatifermentans G17T (= KCTC 15066T, 96.1%), Anaerotignum propionicum DSM 1682T (= KCTC 5582T, 94.9%), Anaerotignum neopropionicum DSM 03847T (= KCTC 15564T, 94.9%), and Anaerotignum aminivorans SH021T (= KCTC 15705T, 94.8%).
31680219	5	41	dep	1682T	743:747	arg1	%					768:768	94.9%	764:768	94.9%	764:768	Analysis of phylogenetic trees based on the 16S rRNA gene sequences revealed that strain KGMB03357T forms a lineage within the genus Anaerotignum, and is most closely related to Anaerotignum lactatifermentans G17T (= KCTC 15066T, 96.1%), Anaerotignum propionicum DSM 1682T (= KCTC 5582T, 94.9%), Anaerotignum neopropionicum DSM 03847T (= KCTC 15564T, 94.9%), and Anaerotignum aminivorans SH021T (= KCTC 15705T, 94.8%).
31680219	10	42	theme	faecicola	1507:1515	arg1	sp					1517:1518	the name Anaerotignum faecicola sp	1485:1518	the name Anaerotignum faecicola sp	1485:1518	Based on the phenotypic, phylogenetic, and genomic properties, strain KGMB 03357T represents a novel species of the genus Anaerotignum, for which the name Anaerotignum faecicola sp.
31680219	7	43	theme	C	1065:1065	arg1	content					1067:1073	The DNA G + C content	1053:1073	content	1067:1073	The DNA G + C content based on the whole-genome sequence is 47.3 mol%.
31680219	2	44	theme	strain	107:112	arg1	KGMB-03357T					114:124	strain KGMB-03357T	107:124	strain KGMB-03357T	107:124	A strictly anaerobic bacterium, designated as strain KGMB-03357T, was isolated from the faeces of a healthy Korean selected by Bundang Seoul National University based on health status.
31680219	10	45	theme	name	1489:1492	arg1	sp					1517:1518	the name Anaerotignum faecicola sp	1485:1518	the name Anaerotignum faecicola sp	1485:1518	Based on the phenotypic, phylogenetic, and genomic properties, strain KGMB 03357T represents a novel species of the genus Anaerotignum, for which the name Anaerotignum faecicola sp.
31680219	6	46	theme	strain	918:923	arg1	03357T					930:935	strain KGMB 03357T	918:935	strain KGMB 03357T	918:935	The ANI values between strain KGMB 03357T and members of the genus Anaerotignum were 73.3-71.0%, which are below the ANI criterion for interspecies identity.
31680219	5	47	dep	Anaerotignum	714:725	arg1	propionicum					727:737	propionicum	727:737	propionicum	727:737	Analysis of phylogenetic trees based on the 16S rRNA gene sequences revealed that strain KGMB03357T forms a lineage within the genus Anaerotignum, and is most closely related to Anaerotignum lactatifermentans G17T (= KCTC 15066T, 96.1%), Anaerotignum propionicum DSM 1682T (= KCTC 5582T, 94.9%), Anaerotignum neopropionicum DSM 03847T (= KCTC 15564T, 94.9%), and Anaerotignum aminivorans SH021T (= KCTC 15705T, 94.8%).
31680219	9	48	from	acid	1302:1305	arg1	peptidoglycan					1324:1336	the cell wall peptidoglycan	1310:1336	the cell wall peptidoglycan	1310:1336	Strain KGMB03357T contains meso-diaminopimelic acid as the diagnostic amino acid in the cell wall peptidoglycan.
31680219	6	49	theme	genus	956:960	arg1	Anaerotignum					962:973	the genus Anaerotignum	952:973	the genus Anaerotignum	952:973	The ANI values between strain KGMB 03357T and members of the genus Anaerotignum were 73.3-71.0%, which are below the ANI criterion for interspecies identity.
31680219	10	50	theme	genomic	1382:1388	arg1	properties					1390:1399	the phenotypic, phylogenetic, and genomic properties	1348:1399	the phenotypic, phylogenetic, and genomic properties	1348:1399	Based on the phenotypic, phylogenetic, and genomic properties, strain KGMB 03357T represents a novel species of the genus Anaerotignum, for which the name Anaerotignum faecicola sp.
31680219	3	51	theme	strain	255:260	arg1	KGMB03357T					262:271	strain KGMB03357T	255:271	strain KGMB03357T	255:271	Cells of strain KGMB03357T are Gram-stain-positive, non-motile, non-spore-forming, and observed as straight or curved rods.
31680219	4	52	theme	40°C	422:425	arg1	temperature					407:417	optimum temperature	399:417	optimum temperature of 40°C	399:425	The isolate grew at 10-45°C (optimum temperature of 40°C) and a pH range of 5.1-10.5 (optimum pH of 6.8).
31680219	6	53	theme	Anaerotignum	962:973	arg1	03357T					930:935	strain KGMB 03357T	918:935	strain KGMB 03357T	918:935	The ANI values between strain KGMB 03357T and members of the genus Anaerotignum were 73.3-71.0%, which are below the ANI criterion for interspecies identity.
31680219	6	53	theme	Anaerotignum	962:973	arg1	members					941:947	members	941:947	members of the genus Anaerotignum	941:973	The ANI values between strain KGMB 03357T and members of the genus Anaerotignum were 73.3-71.0%, which are below the ANI criterion for interspecies identity.
31680219	10	54	theme	KGMB	1409:1412	arg1	03357T					1414:1419	strain KGMB 03357T	1402:1419	strain KGMB 03357T	1402:1419	Based on the phenotypic, phylogenetic, and genomic properties, strain KGMB 03357T represents a novel species of the genus Anaerotignum, for which the name Anaerotignum faecicola sp.
31680219	5	55	theme	=	750:750	arg1	5582T					757:761	= KCTC 5582T	750:761	= KCTC 5582T	750:761	Analysis of phylogenetic trees based on the 16S rRNA gene sequences revealed that strain KGMB03357T forms a lineage within the genus Anaerotignum, and is most closely related to Anaerotignum lactatifermentans G17T (= KCTC 15066T, 96.1%), Anaerotignum propionicum DSM 1682T (= KCTC 5582T, 94.9%), Anaerotignum neopropionicum DSM 03847T (= KCTC 15564T, 94.9%), and Anaerotignum aminivorans SH021T (= KCTC 15705T, 94.8%).
31680219	5	55	theme	=	750:750	arg1	%					768:768	94.9%	764:768	94.9%	764:768	Analysis of phylogenetic trees based on the 16S rRNA gene sequences revealed that strain KGMB03357T forms a lineage within the genus Anaerotignum, and is most closely related to Anaerotignum lactatifermentans G17T (= KCTC 15066T, 96.1%), Anaerotignum propionicum DSM 1682T (= KCTC 5582T, 94.9%), Anaerotignum neopropionicum DSM 03847T (= KCTC 15564T, 94.9%), and Anaerotignum aminivorans SH021T (= KCTC 15705T, 94.8%).
31680219	8	56	theme	strain	1158:1163	arg1	KGMB03357T					1165:1174	strain KGMB03357T	1158:1174	strain KGMB03357T	1158:1174	The major cellular fatty acids of strain KGMB03357T are C16:0, C18:0, C18∶1 cis 9, and anteiso-C15∶0.
31680219	5	57	theme	DSM	800:802	arg1	03847T					804:809	Anaerotignum neopropionicum DSM 03847T	772:809	Anaerotignum neopropionicum DSM 03847T (= KCTC 15564T, 94.9%)	772:832	Analysis of phylogenetic trees based on the 16S rRNA gene sequences revealed that strain KGMB03357T forms a lineage within the genus Anaerotignum, and is most closely related to Anaerotignum lactatifermentans G17T (= KCTC 15066T, 96.1%), Anaerotignum propionicum DSM 1682T (= KCTC 5582T, 94.9%), Anaerotignum neopropionicum DSM 03847T (= KCTC 15564T, 94.9%), and Anaerotignum aminivorans SH021T (= KCTC 15705T, 94.8%).
31680219	6	58	theme	interspecies	1030:1041	arg1	identity					1043:1050	interspecies identity	1030:1050	interspecies identity	1030:1050	The ANI values between strain KGMB 03357T and members of the genus Anaerotignum were 73.3-71.0%, which are below the ANI criterion for interspecies identity.
31680219	5	59	theme	trees	501:505	arg1	Analysis					476:483	Analysis	476:483	Analysis of phylogenetic trees based on the 16S rRNA gene sequences	476:542	Analysis of phylogenetic trees based on the 16S rRNA gene sequences revealed that strain KGMB03357T forms a lineage within the genus Anaerotignum, and is most closely related to Anaerotignum lactatifermentans G17T (= KCTC 15066T, 96.1%), Anaerotignum propionicum DSM 1682T (= KCTC 5582T, 94.9%), Anaerotignum neopropionicum DSM 03847T (= KCTC 15564T, 94.9%), and Anaerotignum aminivorans SH021T (= KCTC 15705T, 94.8%).
31680219	9	60	theme	diagnostic	1285:1294	arg1	acid					1302:1305	the diagnostic amino acid	1281:1305	the diagnostic amino acid in the cell wall peptidoglycan	1281:1336	Strain KGMB03357T contains meso-diaminopimelic acid as the diagnostic amino acid in the cell wall peptidoglycan.
31680219	9	60	theme	diagnostic	1285:1294	arg1	acid					1273:1276	meso-diaminopimelic acid	1253:1276	meso-diaminopimelic acid	1253:1276	Strain KGMB03357T contains meso-diaminopimelic acid as the diagnostic amino acid in the cell wall peptidoglycan.
31680219	5	61	dep	Anaerotignum	654:665	arg1	lactatifermentans					667:683	lactatifermentans	667:683	lactatifermentans	667:683	Analysis of phylogenetic trees based on the 16S rRNA gene sequences revealed that strain KGMB03357T forms a lineage within the genus Anaerotignum, and is most closely related to Anaerotignum lactatifermentans G17T (= KCTC 15066T, 96.1%), Anaerotignum propionicum DSM 1682T (= KCTC 5582T, 94.9%), Anaerotignum neopropionicum DSM 03847T (= KCTC 15564T, 94.9%), and Anaerotignum aminivorans SH021T (= KCTC 15705T, 94.8%).
31680219	9	62	theme	wall	1319:1322	arg1	peptidoglycan					1324:1336	the cell wall peptidoglycan	1310:1336	the cell wall peptidoglycan	1310:1336	Strain KGMB03357T contains meso-diaminopimelic acid as the diagnostic amino acid in the cell wall peptidoglycan.
31680219	5	63	theme	KCTC	693:696	arg1	G17T					685:688	Anaerotignum lactatifermentans G17T	654:688	Anaerotignum lactatifermentans G17T (= KCTC 15066T, 96.1%)	654:711	Analysis of phylogenetic trees based on the 16S rRNA gene sequences revealed that strain KGMB03357T forms a lineage within the genus Anaerotignum, and is most closely related to Anaerotignum lactatifermentans G17T (= KCTC 15066T, 96.1%), Anaerotignum propionicum DSM 1682T (= KCTC 5582T, 94.9%), Anaerotignum neopropionicum DSM 03847T (= KCTC 15564T, 94.9%), and Anaerotignum aminivorans SH021T (= KCTC 15705T, 94.8%).
31680219	5	63	theme	KCTC	693:696	arg1	15066T					698:703	= KCTC 15066T	691:703	= KCTC 15066T	691:703	Analysis of phylogenetic trees based on the 16S rRNA gene sequences revealed that strain KGMB03357T forms a lineage within the genus Anaerotignum, and is most closely related to Anaerotignum lactatifermentans G17T (= KCTC 15066T, 96.1%), Anaerotignum propionicum DSM 1682T (= KCTC 5582T, 94.9%), Anaerotignum neopropionicum DSM 03847T (= KCTC 15564T, 94.9%), and Anaerotignum aminivorans SH021T (= KCTC 15705T, 94.8%).
31680219	5	64	theme	Anaerotignum	772:783	arg1	03847T					804:809	Anaerotignum neopropionicum DSM 03847T	772:809	Anaerotignum neopropionicum DSM 03847T (= KCTC 15564T, 94.9%)	772:832	Analysis of phylogenetic trees based on the 16S rRNA gene sequences revealed that strain KGMB03357T forms a lineage within the genus Anaerotignum, and is most closely related to Anaerotignum lactatifermentans G17T (= KCTC 15066T, 96.1%), Anaerotignum propionicum DSM 1682T (= KCTC 5582T, 94.9%), Anaerotignum neopropionicum DSM 03847T (= KCTC 15564T, 94.9%), and Anaerotignum aminivorans SH021T (= KCTC 15705T, 94.8%).
31680219	5	65	dep	15066T	698:703	arg1	%					710:710	96.1%	706:710	96.1%	706:710	Analysis of phylogenetic trees based on the 16S rRNA gene sequences revealed that strain KGMB03357T forms a lineage within the genus Anaerotignum, and is most closely related to Anaerotignum lactatifermentans G17T (= KCTC 15066T, 96.1%), Anaerotignum propionicum DSM 1682T (= KCTC 5582T, 94.9%), Anaerotignum neopropionicum DSM 03847T (= KCTC 15564T, 94.9%), and Anaerotignum aminivorans SH021T (= KCTC 15705T, 94.8%).
31680219	5	66	theme	gene	529:532	arg1	sequences					534:542	the 16S rRNA gene sequences	516:542	the 16S rRNA gene sequences	516:542	Analysis of phylogenetic trees based on the 16S rRNA gene sequences revealed that strain KGMB03357T forms a lineage within the genus Anaerotignum, and is most closely related to Anaerotignum lactatifermentans G17T (= KCTC 15066T, 96.1%), Anaerotignum propionicum DSM 1682T (= KCTC 5582T, 94.9%), Anaerotignum neopropionicum DSM 03847T (= KCTC 15564T, 94.9%), and Anaerotignum aminivorans SH021T (= KCTC 15705T, 94.8%).
31680219	1	67	theme	human	47:51	arg1	faeces					53:58	human faeces	47:58	human faeces	47:58	nov., isolated from human faeces.
31680219	3	68	theme	straight	345:352	arg1	rods					364:367	straight or curved rods	345:367	straight or curved rods	345:367	Cells of strain KGMB03357T are Gram-stain-positive, non-motile, non-spore-forming, and observed as straight or curved rods.
31680219	3	68	theme	straight	345:352	arg1	Cells					246:250	Cells	246:250	Cells of strain KGMB03357T	246:271	Cells of strain KGMB03357T are Gram-stain-positive, non-motile, non-spore-forming, and observed as straight or curved rods.
31680219	4	69	theme	pH	434:435	arg1	range					437:441	a pH range	432:441	a pH range of 5.1-10.5 (optimum pH of 6.8)	432:473	The isolate grew at 10-45°C (optimum temperature of 40°C) and a pH range of 5.1-10.5 (optimum pH of 6.8).
31680219	8	70	theme	major	1128:1132	arg1	C16:0					1180:1184	C16:0	1180:1184	C16:0	1180:1184	The major cellular fatty acids of strain KGMB03357T are C16:0, C18:0, C18∶1 cis 9, and anteiso-C15∶0.
31680219	8	70	theme	major	1128:1132	arg1	acids					1149:1153	The major cellular fatty acids	1124:1153	The major cellular fatty acids of strain KGMB03357T	1124:1174	The major cellular fatty acids of strain KGMB03357T are C16:0, C18:0, C18∶1 cis 9, and anteiso-C15∶0.
31680219	5	71	dep	03847T	804:809	arg1	15564T					819:824	= KCTC 15564T	812:824	= KCTC 15564T	812:824	Analysis of phylogenetic trees based on the 16S rRNA gene sequences revealed that strain KGMB03357T forms a lineage within the genus Anaerotignum, and is most closely related to Anaerotignum lactatifermentans G17T (= KCTC 15066T, 96.1%), Anaerotignum propionicum DSM 1682T (= KCTC 5582T, 94.9%), Anaerotignum neopropionicum DSM 03847T (= KCTC 15564T, 94.9%), and Anaerotignum aminivorans SH021T (= KCTC 15705T, 94.8%).
31680219	5	71	dep	03847T	804:809	arg1	%					831:831	94.9%	827:831	94.9%	827:831	Analysis of phylogenetic trees based on the 16S rRNA gene sequences revealed that strain KGMB03357T forms a lineage within the genus Anaerotignum, and is most closely related to Anaerotignum lactatifermentans G17T (= KCTC 15066T, 96.1%), Anaerotignum propionicum DSM 1682T (= KCTC 5582T, 94.9%), Anaerotignum neopropionicum DSM 03847T (= KCTC 15564T, 94.9%), and Anaerotignum aminivorans SH021T (= KCTC 15705T, 94.8%).
31680219	0	72	theme	faecicola	13:21	arg1	sp					23:24	Anaerotignum faecicola sp	0:24	Anaerotignum faecicola sp.	0:25	Anaerotignum faecicola sp.
31680219	5	73	theme	KCTC	814:817	arg1	15564T					819:824	= KCTC 15564T	812:824	= KCTC 15564T	812:824	Analysis of phylogenetic trees based on the 16S rRNA gene sequences revealed that strain KGMB03357T forms a lineage within the genus Anaerotignum, and is most closely related to Anaerotignum lactatifermentans G17T (= KCTC 15066T, 96.1%), Anaerotignum propionicum DSM 1682T (= KCTC 5582T, 94.9%), Anaerotignum neopropionicum DSM 03847T (= KCTC 15564T, 94.9%), and Anaerotignum aminivorans SH021T (= KCTC 15705T, 94.8%).
31680219	5	73	theme	KCTC	814:817	arg1	%					831:831	94.9%	827:831	94.9%	827:831	Analysis of phylogenetic trees based on the 16S rRNA gene sequences revealed that strain KGMB03357T forms a lineage within the genus Anaerotignum, and is most closely related to Anaerotignum lactatifermentans G17T (= KCTC 15066T, 96.1%), Anaerotignum propionicum DSM 1682T (= KCTC 5582T, 94.9%), Anaerotignum neopropionicum DSM 03847T (= KCTC 15564T, 94.9%), and Anaerotignum aminivorans SH021T (= KCTC 15705T, 94.8%).
31680219	7	74	theme	mol	1118:1120	arg1	%					1121:1121	47.3 mol%	1113:1121	47.3 mol%	1113:1121	The DNA G + C content based on the whole-genome sequence is 47.3 mol%.
31680219	7	74	theme	mol	1118:1120	arg1	G					1061:1061	The DNA G + C content	1053:1073	G	1061:1061	The DNA G + C content based on the whole-genome sequence is 47.3 mol%.
31680219	5	75	theme	16S	520:522	arg1	sequences					534:542	the 16S rRNA gene sequences	516:542	the 16S rRNA gene sequences	516:542	Analysis of phylogenetic trees based on the 16S rRNA gene sequences revealed that strain KGMB03357T forms a lineage within the genus Anaerotignum, and is most closely related to Anaerotignum lactatifermentans G17T (= KCTC 15066T, 96.1%), Anaerotignum propionicum DSM 1682T (= KCTC 5582T, 94.9%), Anaerotignum neopropionicum DSM 03847T (= KCTC 15564T, 94.9%), and Anaerotignum aminivorans SH021T (= KCTC 15705T, 94.8%).
31680219	3	76	theme	curved	357:362	arg1	rods					364:367	straight or curved rods	345:367	straight or curved rods	345:367	Cells of strain KGMB03357T are Gram-stain-positive, non-motile, non-spore-forming, and observed as straight or curved rods.
31680219	3	76	theme	curved	357:362	arg1	Cells					246:250	Cells	246:250	Cells of strain KGMB03357T	246:271	Cells of strain KGMB03357T are Gram-stain-positive, non-motile, non-spore-forming, and observed as straight or curved rods.
31680219	8	77	theme	KGMB03357T	1165:1174	arg1	C16:0					1180:1184	C16:0	1180:1184	C16:0	1180:1184	The major cellular fatty acids of strain KGMB03357T are C16:0, C18:0, C18∶1 cis 9, and anteiso-C15∶0.
31680219	8	77	theme	KGMB03357T	1165:1174	arg1	acids					1149:1153	The major cellular fatty acids	1124:1153	The major cellular fatty acids of strain KGMB03357T	1124:1174	The major cellular fatty acids of strain KGMB03357T are C16:0, C18:0, C18∶1 cis 9, and anteiso-C15∶0.
31680219	9	78	theme	meso-diaminopimelic	1253:1271	arg1	acid					1302:1305	the diagnostic amino acid	1281:1305	the diagnostic amino acid in the cell wall peptidoglycan	1281:1336	Strain KGMB03357T contains meso-diaminopimelic acid as the diagnostic amino acid in the cell wall peptidoglycan.
31680219	9	78	theme	meso-diaminopimelic	1253:1271	arg1	acid					1273:1276	meso-diaminopimelic acid	1253:1276	meso-diaminopimelic acid	1253:1276	Strain KGMB03357T contains meso-diaminopimelic acid as the diagnostic amino acid in the cell wall peptidoglycan.
31680219	5	79	theme	=	872:872	arg1	SH021T					864:869	Anaerotignum aminivorans SH021T	839:869	Anaerotignum aminivorans SH021T (= KCTC 15705T, 94.8%)	839:892	Analysis of phylogenetic trees based on the 16S rRNA gene sequences revealed that strain KGMB03357T forms a lineage within the genus Anaerotignum, and is most closely related to Anaerotignum lactatifermentans G17T (= KCTC 15066T, 96.1%), Anaerotignum propionicum DSM 1682T (= KCTC 5582T, 94.9%), Anaerotignum neopropionicum DSM 03847T (= KCTC 15564T, 94.9%), and Anaerotignum aminivorans SH021T (= KCTC 15705T, 94.8%).
31680219	5	79	theme	=	872:872	arg1	15705T					879:884	= KCTC 15705T	872:884	= KCTC 15705T	872:884	Analysis of phylogenetic trees based on the 16S rRNA gene sequences revealed that strain KGMB03357T forms a lineage within the genus Anaerotignum, and is most closely related to Anaerotignum lactatifermentans G17T (= KCTC 15066T, 96.1%), Anaerotignum propionicum DSM 1682T (= KCTC 5582T, 94.9%), Anaerotignum neopropionicum DSM 03847T (= KCTC 15564T, 94.9%), and Anaerotignum aminivorans SH021T (= KCTC 15705T, 94.8%).
31680219	7	80	theme	whole-genome	1088:1099	arg1	sequence					1101:1108	the whole-genome sequence	1084:1108	the whole-genome sequence	1084:1108	The DNA G + C content based on the whole-genome sequence is 47.3 mol%.
31680219	4	81	theme	6.8	470:472	arg1	pH					464:465	optimum pH	456:465	optimum pH of 6.8	456:472	The isolate grew at 10-45°C (optimum temperature of 40°C) and a pH range of 5.1-10.5 (optimum pH of 6.8).
31680219	12	82	theme	=	1570:1570	arg1	KGMB03357T					1558:1567	KGMB03357T	1558:1567	KGMB03357T (= KCTC 15736T = DSM 107953T)	1558:1597	The type strain is KGMB03357T (= KCTC 15736T = DSM 107953T).
31680219	12	82	theme	=	1570:1570	arg1	107953T					1590:1596	= KCTC 15736T = DSM 107953T	1570:1596	= KCTC 15736T = DSM 107953T	1570:1596	The type strain is KGMB03357T (= KCTC 15736T = DSM 107953T).
31680219	2	83	attach	isolated	131:138	arg2	bacterium					82:90	A strictly anaerobic bacterium	61:90	A strictly anaerobic bacterium	61:90	A strictly anaerobic bacterium, designated as strain KGMB-03357T, was isolated from the faeces of a healthy Korean selected by Bundang Seoul National University based on health status.
31680219	2	83	attach	isolated	131:138	arg1	faeces					149:154	the faeces	145:154	the faeces of a healthy Korean selected by Bundang Seoul National University based on health status	145:243	A strictly anaerobic bacterium, designated as strain KGMB-03357T, was isolated from the faeces of a healthy Korean selected by Bundang Seoul National University based on health status.
31680219	4	84	dep	5.1-10.5	446:453	arg1	pH					464:465	optimum pH	456:465	optimum pH of 6.8	456:472	The isolate grew at 10-45°C (optimum temperature of 40°C) and a pH range of 5.1-10.5 (optimum pH of 6.8).
31680219	5	85	theme	Anaerotignum	839:850	arg1	SH021T					864:869	Anaerotignum aminivorans SH021T	839:869	Anaerotignum aminivorans SH021T (= KCTC 15705T, 94.8%)	839:892	Analysis of phylogenetic trees based on the 16S rRNA gene sequences revealed that strain KGMB03357T forms a lineage within the genus Anaerotignum, and is most closely related to Anaerotignum lactatifermentans G17T (= KCTC 15066T, 96.1%), Anaerotignum propionicum DSM 1682T (= KCTC 5582T, 94.9%), Anaerotignum neopropionicum DSM 03847T (= KCTC 15564T, 94.9%), and Anaerotignum aminivorans SH021T (= KCTC 15705T, 94.8%).
31680219	5	85	theme	Anaerotignum	839:850	arg1	15705T					879:884	= KCTC 15705T	872:884	= KCTC 15705T	872:884	Analysis of phylogenetic trees based on the 16S rRNA gene sequences revealed that strain KGMB03357T forms a lineage within the genus Anaerotignum, and is most closely related to Anaerotignum lactatifermentans G17T (= KCTC 15066T, 96.1%), Anaerotignum propionicum DSM 1682T (= KCTC 5582T, 94.9%), Anaerotignum neopropionicum DSM 03847T (= KCTC 15564T, 94.9%), and Anaerotignum aminivorans SH021T (= KCTC 15705T, 94.8%).
31680219	12	86	theme	15736T	1577:1582	arg1	KGMB03357T					1558:1567	KGMB03357T	1558:1567	KGMB03357T (= KCTC 15736T = DSM 107953T)	1558:1597	The type strain is KGMB03357T (= KCTC 15736T = DSM 107953T).
31680219	12	86	theme	15736T	1577:1582	arg1	107953T					1590:1596	= KCTC 15736T = DSM 107953T	1570:1596	= KCTC 15736T = DSM 107953T	1570:1596	The type strain is KGMB03357T (= KCTC 15736T = DSM 107953T).
31680219	8	87	theme	fatty	1143:1147	arg1	C16:0					1180:1184	C16:0	1180:1184	C16:0	1180:1184	The major cellular fatty acids of strain KGMB03357T are C16:0, C18:0, C18∶1 cis 9, and anteiso-C15∶0.
31680219	8	87	theme	fatty	1143:1147	arg1	acids					1149:1153	The major cellular fatty acids	1124:1153	The major cellular fatty acids of strain KGMB03357T	1124:1174	The major cellular fatty acids of strain KGMB03357T are C16:0, C18:0, C18∶1 cis 9, and anteiso-C15∶0.
31680219	2	88	theme	Korean	169:174	arg1	faeces					149:154	the faeces	145:154	the faeces of a healthy Korean selected by Bundang Seoul National University based on health status	145:243	A strictly anaerobic bacterium, designated as strain KGMB-03357T, was isolated from the faeces of a healthy Korean selected by Bundang Seoul National University based on health status.
31680219	10	89	theme	Anaerotignum	1461:1472	arg1	species					1440:1446	a novel species	1432:1446	a novel species	1432:1446	Based on the phenotypic, phylogenetic, and genomic properties, strain KGMB 03357T represents a novel species of the genus Anaerotignum, for which the name Anaerotignum faecicola sp.
31680219	10	90	theme	Anaerotignum	1494:1505	arg1	sp					1517:1518	the name Anaerotignum faecicola sp	1485:1518	the name Anaerotignum faecicola sp	1485:1518	Based on the phenotypic, phylogenetic, and genomic properties, strain KGMB 03357T represents a novel species of the genus Anaerotignum, for which the name Anaerotignum faecicola sp.
31680219	4	91	dep	10-45°C	390:396	arg1	temperature					407:417	optimum temperature	399:417	optimum temperature of 40°C	399:425	The isolate grew at 10-45°C (optimum temperature of 40°C) and a pH range of 5.1-10.5 (optimum pH of 6.8).
31652274	7	0	theme	functional	1066:1075	arg1	demonstration					1077:1089	the first functional demonstration that overexpression of S100A4, a calcium-binding protein that is frequently overexpressed in metastatic tumors and inhibits NMIIA activity by inducing filament disassembly, effectively reduces cell contractility	1056:1301	the first functional demonstration that overexpression of S100A4, a calcium-binding protein that is frequently overexpressed in metastatic tumors and inhibits NMIIA activity by inducing filament disassembly, effectively reduces cell contractility	1056:1301	Using Matrigel patterning as a bioassay, we provide the first functional demonstration that overexpression of S100A4, a calcium-binding protein that is frequently overexpressed in metastatic tumors and inhibits NMIIA activity by inducing filament disassembly, effectively reduces cell contractility.
31652274	1	1	dep	-induced	85:92	arg1	II					76:77	Non-muscle myosin II	58:77	Non-muscle myosin II	58:77	Non-muscle myosin II (NMII)-induced multicellular contractility is essential for development, maintenance and remodeling of tissue morphologies.
31652274	7	2	theme	cell	1284:1287	arg1	contractility					1289:1301	cell contractility	1284:1301	cell contractility	1284:1301	Using Matrigel patterning as a bioassay, we provide the first functional demonstration that overexpression of S100A4, a calcium-binding protein that is frequently overexpressed in metastatic tumors and inhibits NMIIA activity by inducing filament disassembly, effectively reduces cell contractility.
31652274	6	3	from	cells	916:920	arg1	inhibition					859:868	inhibition	859:868	inhibition of NMII activity in A431 epithelial carcinoma cells	859:920	The assay was calibrated by inhibition of NMII activity in A431 epithelial carcinoma cells either directly with blebbistatin or indirectly with Y27632 Rho kinase inhibitor.
31652274	4	4	theme	computational	516:528	arg1	model					530:534	a computational model	514:534	a computational model to explore how cell-exerted contractile forces can tear up the cell-Matrigel composite material and gradually remodel it into a network structure	514:680	We propose a computational model to explore how cell-exerted contractile forces can tear up the cell-Matrigel composite material and gradually remodel it into a network structure.
31652274	7	5	theme	Matrigel	1010:1017	arg1	patterning					1019:1028	Matrigel patterning	1010:1028	Matrigel patterning	1010:1028	Using Matrigel patterning as a bioassay, we provide the first functional demonstration that overexpression of S100A4, a calcium-binding protein that is frequently overexpressed in metastatic tumors and inhibits NMIIA activity by inducing filament disassembly, effectively reduces cell contractility.
31652274	7	5	theme	Matrigel	1010:1017	arg1	bioassay					1035:1042	a bioassay	1033:1042	a bioassay	1033:1042	Using Matrigel patterning as a bioassay, we provide the first functional demonstration that overexpression of S100A4, a calcium-binding protein that is frequently overexpressed in metastatic tumors and inhibits NMIIA activity by inducing filament disassembly, effectively reduces cell contractility.
31652274	4	6	theme	composite	613:621	arg1	material					623:630	the cell-Matrigel composite material	595:630	the cell-Matrigel composite material	595:630	We propose a computational model to explore how cell-exerted contractile forces can tear up the cell-Matrigel composite material and gradually remodel it into a network structure.
31652274	4	7	dep	tear	587:590	arg1	up					592:593	up	592:593	up	592:593	We propose a computational model to explore how cell-exerted contractile forces can tear up the cell-Matrigel composite material and gradually remodel it into a network structure.
31652274	6	8	theme	Y27632	975:980	arg1	inhibitor					993:1001	Y27632 Rho kinase inhibitor	975:1001	Y27632 Rho kinase inhibitor	975:1001	The assay was calibrated by inhibition of NMII activity in A431 epithelial carcinoma cells either directly with blebbistatin or indirectly with Y27632 Rho kinase inhibitor.
31652274	4	9	theme	cell-Matrigel	599:611	arg1	material					623:630	the cell-Matrigel composite material	595:630	the cell-Matrigel composite material	595:630	We propose a computational model to explore how cell-exerted contractile forces can tear up the cell-Matrigel composite material and gradually remodel it into a network structure.
31652274	7	10	theme	filament	1242:1249	arg1	disassembly					1251:1261	filament disassembly	1242:1261	filament disassembly	1242:1261	Using Matrigel patterning as a bioassay, we provide the first functional demonstration that overexpression of S100A4, a calcium-binding protein that is frequently overexpressed in metastatic tumors and inhibits NMIIA activity by inducing filament disassembly, effectively reduces cell contractility.
31652274	1	11	theme	tissue	182:187	arg1	morphologies					189:200	tissue morphologies	182:200	tissue morphologies	182:200	Non-muscle myosin II (NMII)-induced multicellular contractility is essential for development, maintenance and remodeling of tissue morphologies.
31652274	3	12	theme	endothelial	372:382	arg1	cells					384:388	endothelial cells	372:388	endothelial cells	372:388	We demonstrate that the Matrigel patterning assay, widely used to characterize endothelial cells, is a highly sensitive tool to evaluate cell contractility within a soft extracellular matrix (ECM) environment.
31652274	6	13	from	inhibition	859:868	arg1	cells					916:920	A431 epithelial carcinoma cells	890:920	A431 epithelial carcinoma cells	890:920	The assay was calibrated by inhibition of NMII activity in A431 epithelial carcinoma cells either directly with blebbistatin or indirectly with Y27632 Rho kinase inhibitor.
31652274	0	14	theme	Matrigel	0:7	arg1	patterning					9:18	Matrigel patterning	0:18	Matrigel patterning	0:18	Matrigel patterning reflects multicellular contractility.
31652274	1	15	theme	morphologies	189:200	arg1	development					139:149	development	139:149	development	139:149	Non-muscle myosin II (NMII)-induced multicellular contractility is essential for development, maintenance and remodeling of tissue morphologies.
31652274	1	15	theme	morphologies	189:200	arg1	remodeling					168:177	remodeling	168:177	remodeling	168:177	Non-muscle myosin II (NMII)-induced multicellular contractility is essential for development, maintenance and remodeling of tissue morphologies.
31652274	1	15	theme	morphologies	189:200	arg1	maintenance					152:162	maintenance	152:162	maintenance	152:162	Non-muscle myosin II (NMII)-induced multicellular contractility is essential for development, maintenance and remodeling of tissue morphologies.
31652274	6	16	theme	carcinoma	906:914	arg1	cells					916:920	A431 epithelial carcinoma cells	890:920	A431 epithelial carcinoma cells	890:920	The assay was calibrated by inhibition of NMII activity in A431 epithelial carcinoma cells either directly with blebbistatin or indirectly with Y27632 Rho kinase inhibitor.
31652274	1	17	theme	-induced	85:92	arg1	contractility					108:120	Non-muscle myosin II (NMII)-induced multicellular contractility	58:120	Non-muscle myosin II (NMII)-induced multicellular contractility	58:120	Non-muscle myosin II (NMII)-induced multicellular contractility is essential for development, maintenance and remodeling of tissue morphologies.
31652274	3	18	theme	sensitive	403:411	arg1	tool					413:416	a highly sensitive tool	394:416	a highly sensitive tool to evaluate cell contractility within a soft extracellular matrix (ECM) environment	394:500	We demonstrate that the Matrigel patterning assay, widely used to characterize endothelial cells, is a highly sensitive tool to evaluate cell contractility within a soft extracellular matrix (ECM) environment.
31652274	3	18	theme	sensitive	403:411	arg1	assay					337:341	the Matrigel patterning assay	313:341	the Matrigel patterning assay	313:341	We demonstrate that the Matrigel patterning assay, widely used to characterize endothelial cells, is a highly sensitive tool to evaluate cell contractility within a soft extracellular matrix (ECM) environment.
31652274	7	19	theme	calcium-binding	1124:1138	arg1	protein					1140:1146	a calcium-binding protein	1122:1146	a calcium-binding protein that is frequently overexpressed in metastatic tumors and inhibits NMIIA activity by inducing filament disassembly	1122:1261	Using Matrigel patterning as a bioassay, we provide the first functional demonstration that overexpression of S100A4, a calcium-binding protein that is frequently overexpressed in metastatic tumors and inhibits NMIIA activity by inducing filament disassembly, effectively reduces cell contractility.
31652274	7	19	theme	calcium-binding	1124:1138	arg1	S100A4					1114:1119	S100A4	1114:1119	S100A4	1114:1119	Using Matrigel patterning as a bioassay, we provide the first functional demonstration that overexpression of S100A4, a calcium-binding protein that is frequently overexpressed in metastatic tumors and inhibits NMIIA activity by inducing filament disassembly, effectively reduces cell contractility.
31652274	1	20	theme	multicellular	94:106	arg1	contractility					108:120	Non-muscle myosin II (NMII)-induced multicellular contractility	58:120	Non-muscle myosin II (NMII)-induced multicellular contractility	58:120	Non-muscle myosin II (NMII)-induced multicellular contractility is essential for development, maintenance and remodeling of tissue morphologies.
31652274	6	21	theme	A431	890:893	arg1	cells					916:920	A431 epithelial carcinoma cells	890:920	A431 epithelial carcinoma cells	890:920	The assay was calibrated by inhibition of NMII activity in A431 epithelial carcinoma cells either directly with blebbistatin or indirectly with Y27632 Rho kinase inhibitor.
31652274	0	22	theme	multicellular	29:41	arg1	contractility					43:55	multicellular contractility	29:55	multicellular contractility	29:55	Matrigel patterning reflects multicellular contractility.
31652274	4	23	theme	contractile	564:574	arg1	forces					576:581	cell-exerted contractile forces	551:581	cell-exerted contractile forces	551:581	We propose a computational model to explore how cell-exerted contractile forces can tear up the cell-Matrigel composite material and gradually remodel it into a network structure.
31652274	7	24	theme	S100A4	1114:1119	arg1	overexpression					1096:1109	overexpression	1096:1109	overexpression of S100A4, a calcium-binding protein that is frequently overexpressed in metastatic tumors and inhibits NMIIA activity by inducing filament disassembly,	1096:1262	Using Matrigel patterning as a bioassay, we provide the first functional demonstration that overexpression of S100A4, a calcium-binding protein that is frequently overexpressed in metastatic tumors and inhibits NMIIA activity by inducing filament disassembly, effectively reduces cell contractility.
31652274	5	25	theme	cellular	732:739	arg1	contractility					741:753	cellular contractility	732:753	cellular contractility	732:753	We identify measures that are characteristic for cellular contractility and can be obtained from image analysis of the recorded patterning process.
31652274	6	26	theme	epithelial	895:904	arg1	cells					916:920	A431 epithelial carcinoma cells	890:920	A431 epithelial carcinoma cells	890:920	The assay was calibrated by inhibition of NMII activity in A431 epithelial carcinoma cells either directly with blebbistatin or indirectly with Y27632 Rho kinase inhibitor.
31652274	6	27	from	activity	878:885	arg1	cells					916:920	A431 epithelial carcinoma cells	890:920	A431 epithelial carcinoma cells	890:920	The assay was calibrated by inhibition of NMII activity in A431 epithelial carcinoma cells either directly with blebbistatin or indirectly with Y27632 Rho kinase inhibitor.
31652274	5	28	theme	recorded	802:809	arg1	process					822:828	the recorded patterning process	798:828	the recorded patterning process	798:828	We identify measures that are characteristic for cellular contractility and can be obtained from image analysis of the recorded patterning process.
31652274	4	29	theme	network	664:670	arg1	structure					672:680	a network structure	662:680	a network structure	662:680	We propose a computational model to explore how cell-exerted contractile forces can tear up the cell-Matrigel composite material and gradually remodel it into a network structure.
31652274	3	30	theme	extracellular	463:475	arg1	matrix					477:482	extracellular matrix	463:482	a soft extracellular matrix (ECM) environment	456:500	We demonstrate that the Matrigel patterning assay, widely used to characterize endothelial cells, is a highly sensitive tool to evaluate cell contractility within a soft extracellular matrix (ECM) environment.
31652274	3	30	theme	extracellular	463:475	arg1	ECM					485:487	ECM	485:487	ECM	485:487	We demonstrate that the Matrigel patterning assay, widely used to characterize endothelial cells, is a highly sensitive tool to evaluate cell contractility within a soft extracellular matrix (ECM) environment.
31652274	3	31	theme	cell	430:433	arg1	contractility					435:447	cell contractility	430:447	cell contractility	430:447	We demonstrate that the Matrigel patterning assay, widely used to characterize endothelial cells, is a highly sensitive tool to evaluate cell contractility within a soft extracellular matrix (ECM) environment.
31652274	6	32	theme	kinase	986:991	arg1	inhibitor					993:1001	Y27632 Rho kinase inhibitor	975:1001	Y27632 Rho kinase inhibitor	975:1001	The assay was calibrated by inhibition of NMII activity in A431 epithelial carcinoma cells either directly with blebbistatin or indirectly with Y27632 Rho kinase inhibitor.
31652274	5	33	theme	patterning	811:820	arg1	process					822:828	the recorded patterning process	798:828	the recorded patterning process	798:828	We identify measures that are characteristic for cellular contractility and can be obtained from image analysis of the recorded patterning process.
31652274	2	34	theme	birth	249:253	arg1	defects					255:261	birth defects	249:261	birth defects	249:261	Dysregulation of the cytoskeleton can lead to birth defects or enable cancer progression.
31652274	3	35	theme	matrix	477:482	arg1	environment					490:500	a soft extracellular matrix (ECM) environment	456:500	a soft extracellular matrix (ECM) environment	456:500	We demonstrate that the Matrigel patterning assay, widely used to characterize endothelial cells, is a highly sensitive tool to evaluate cell contractility within a soft extracellular matrix (ECM) environment.
31652274	5	36	theme	process	822:828	arg1	analysis					786:793	image analysis	780:793	image analysis of the recorded patterning process	780:828	We identify measures that are characteristic for cellular contractility and can be obtained from image analysis of the recorded patterning process.
31652274	6	37	theme	Rho	982:984	arg1	inhibitor					993:1001	Y27632 Rho kinase inhibitor	975:1001	Y27632 Rho kinase inhibitor	975:1001	The assay was calibrated by inhibition of NMII activity in A431 epithelial carcinoma cells either directly with blebbistatin or indirectly with Y27632 Rho kinase inhibitor.
31652274	3	38	theme	patterning	326:335	arg1	tool					413:416	a highly sensitive tool	394:416	a highly sensitive tool to evaluate cell contractility within a soft extracellular matrix (ECM) environment	394:500	We demonstrate that the Matrigel patterning assay, widely used to characterize endothelial cells, is a highly sensitive tool to evaluate cell contractility within a soft extracellular matrix (ECM) environment.
31652274	3	38	theme	patterning	326:335	arg1	assay					337:341	the Matrigel patterning assay	313:341	the Matrigel patterning assay	313:341	We demonstrate that the Matrigel patterning assay, widely used to characterize endothelial cells, is a highly sensitive tool to evaluate cell contractility within a soft extracellular matrix (ECM) environment.
31652274	6	39	theme	activity	878:885	arg1	inhibition					859:868	inhibition	859:868	inhibition of NMII activity in A431 epithelial carcinoma cells	859:920	The assay was calibrated by inhibition of NMII activity in A431 epithelial carcinoma cells either directly with blebbistatin or indirectly with Y27632 Rho kinase inhibitor.
31652274	4	40	theme	cell-exerted	551:562	arg1	forces					576:581	cell-exerted contractile forces	551:581	cell-exerted contractile forces	551:581	We propose a computational model to explore how cell-exerted contractile forces can tear up the cell-Matrigel composite material and gradually remodel it into a network structure.
31652274	6	41	theme	NMII	873:876	arg1	activity					878:885	NMII activity	873:885	NMII activity in A431 epithelial carcinoma cells	873:920	The assay was calibrated by inhibition of NMII activity in A431 epithelial carcinoma cells either directly with blebbistatin or indirectly with Y27632 Rho kinase inhibitor.
31652274	7	42	theme	NMIIA	1215:1219	arg1	activity					1221:1228	NMIIA activity	1215:1228	NMIIA activity	1215:1228	Using Matrigel patterning as a bioassay, we provide the first functional demonstration that overexpression of S100A4, a calcium-binding protein that is frequently overexpressed in metastatic tumors and inhibits NMIIA activity by inducing filament disassembly, effectively reduces cell contractility.
31652274	7	43	theme	first	1060:1064	arg1	demonstration					1077:1089	the first functional demonstration that overexpression of S100A4, a calcium-binding protein that is frequently overexpressed in metastatic tumors and inhibits NMIIA activity by inducing filament disassembly, effectively reduces cell contractility	1056:1301	the first functional demonstration that overexpression of S100A4, a calcium-binding protein that is frequently overexpressed in metastatic tumors and inhibits NMIIA activity by inducing filament disassembly, effectively reduces cell contractility	1056:1301	Using Matrigel patterning as a bioassay, we provide the first functional demonstration that overexpression of S100A4, a calcium-binding protein that is frequently overexpressed in metastatic tumors and inhibits NMIIA activity by inducing filament disassembly, effectively reduces cell contractility.
31652274	2	44	theme	cytoskeleton	224:235	arg1	Dysregulation					203:215	Dysregulation	203:215	Dysregulation of the cytoskeleton	203:235	Dysregulation of the cytoskeleton can lead to birth defects or enable cancer progression.
31652274	3	45	theme	Matrigel	317:324	arg1	tool					413:416	a highly sensitive tool	394:416	a highly sensitive tool to evaluate cell contractility within a soft extracellular matrix (ECM) environment	394:500	We demonstrate that the Matrigel patterning assay, widely used to characterize endothelial cells, is a highly sensitive tool to evaluate cell contractility within a soft extracellular matrix (ECM) environment.
31652274	3	45	theme	Matrigel	317:324	arg1	assay					337:341	the Matrigel patterning assay	313:341	the Matrigel patterning assay	313:341	We demonstrate that the Matrigel patterning assay, widely used to characterize endothelial cells, is a highly sensitive tool to evaluate cell contractility within a soft extracellular matrix (ECM) environment.
31652274	2	46	theme	cancer	273:278	arg1	progression					280:290	cancer progression	273:290	cancer progression	273:290	Dysregulation of the cytoskeleton can lead to birth defects or enable cancer progression.
31652274	5	47	theme	image	780:784	arg1	analysis					786:793	image analysis	780:793	image analysis of the recorded patterning process	780:828	We identify measures that are characteristic for cellular contractility and can be obtained from image analysis of the recorded patterning process.
31652274	1	48	theme	Non-muscle	58:67	arg1	II					76:77	Non-muscle myosin II	58:77	Non-muscle myosin II	58:77	Non-muscle myosin II (NMII)-induced multicellular contractility is essential for development, maintenance and remodeling of tissue morphologies.
31652274	3	49	theme	soft	458:461	arg1	environment					490:500	a soft extracellular matrix (ECM) environment	456:500	a soft extracellular matrix (ECM) environment	456:500	We demonstrate that the Matrigel patterning assay, widely used to characterize endothelial cells, is a highly sensitive tool to evaluate cell contractility within a soft extracellular matrix (ECM) environment.
31652274	7	50	theme	metastatic	1184:1193	arg1	tumors					1195:1200	metastatic tumors	1184:1200	metastatic tumors	1184:1200	Using Matrigel patterning as a bioassay, we provide the first functional demonstration that overexpression of S100A4, a calcium-binding protein that is frequently overexpressed in metastatic tumors and inhibits NMIIA activity by inducing filament disassembly, effectively reduces cell contractility.
31652274	1	51	theme	myosin	69:74	arg1	II					76:77	Non-muscle myosin II	58:77	Non-muscle myosin II	58:77	Non-muscle myosin II (NMII)-induced multicellular contractility is essential for development, maintenance and remodeling of tissue morphologies.
29974366	11	0	theme	Verrucosispora	1172:1185	arg1	4.3550T					1203:1209	Verrucosispora sediminis CGMCC 4.3550T	1172:1209	Verrucosispora sediminis CGMCC 4.3550T (96.9%)	1172:1217	The strain also showed high 16S rRNA gene sequence similarities to Verrucosispora sediminis CGMCC 4.3550T (96.9%) and Micromonospora tulbaghiae DSM 45142T (96.8%).
29974366	11	0	theme	Verrucosispora	1172:1185	arg1	%					1216:1216	96.9%	1212:1216	96.9%	1212:1216	The strain also showed high 16S rRNA gene sequence similarities to Verrucosispora sediminis CGMCC 4.3550T (96.9%) and Micromonospora tulbaghiae DSM 45142T (96.8%).
29974366	10	1	theme	strain	1014:1019	arg1	CF22T					1025:1029	strain YIM CF22T	1014:1029	strain YIM CF22T	1014:1029	16S rRNA gene sequence analysis of strain YIM CF22T showed high similarity (97.0%) to Actinoplanes rishiriensis NBRC 108556T.
29974366	5	2	theme	cell	444:447	arg1	peptidoglycan					454:466	the cell wall peptidoglycan	440:466	the cell wall peptidoglycan	440:466	It contains meso-diaminopimelic acid in the cell wall peptidoglycan.
29974366	7	3	theme	cell	702:705	arg1	sugars					707:712	The whole cell sugars	692:712	The whole cell sugars	692:712	The whole cell sugars were found to be ribose, mannose, galactose, glucose and xylose.
29974366	7	3	theme	cell	702:705	arg1	ribose					731:736	ribose	731:736	ribose	731:736	The whole cell sugars were found to be ribose, mannose, galactose, glucose and xylose.
29974366	11	4	theme	gene	1142:1145	arg1	similarities					1156:1167	high 16S rRNA gene sequence similarities	1128:1167	high 16S rRNA gene sequence similarities to Verrucosispora sediminis CGMCC 4.3550T (96.9%) and Micromonospora tulbaghiae DSM 45142T (96.8%)	1128:1266	The strain also showed high 16S rRNA gene sequence similarities to Verrucosispora sediminis CGMCC 4.3550T (96.9%) and Micromonospora tulbaghiae DSM 45142T (96.8%).
29974366	13	5	dep	characteristics	1515:1529	arg1	the					1487:1489	the	1487:1489	the	1487:1489	Based on the differential phenotypic characteristics and the results of DNA-DNA relatedness and phylogenetic analysis, it is proposed that strain YIM CF22T represents a novel species of the genus Actinoplanes, for which the name Actinoplanes deserti sp.
29974366	12	6	with	clusters	1359:1366	arg1	108556T					1394:1400	108556T	1394:1400	108556T	1394:1400	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain YIM CF22T clusters with A. rishiriensis NBRC 108556T, Actinoplanes globisporus JCM 3186T and Actinoplanes rhizophilus NEAU-A-2T.
29974366	11	7	theme	16S	1133:1135	arg1	similarities					1156:1167	high 16S rRNA gene sequence similarities	1128:1167	high 16S rRNA gene sequence similarities to Verrucosispora sediminis CGMCC 4.3550T (96.9%) and Micromonospora tulbaghiae DSM 45142T (96.8%)	1128:1266	The strain also showed high 16S rRNA gene sequence similarities to Verrucosispora sediminis CGMCC 4.3550T (96.9%) and Micromonospora tulbaghiae DSM 45142T (96.8%).
29974366	5	8	theme	meso-diaminopimelic	412:430	arg1	acid					432:435	meso-diaminopimelic acid	412:435	meso-diaminopimelic acid in the cell wall peptidoglycan	412:466	It contains meso-diaminopimelic acid in the cell wall peptidoglycan.
29974366	3	9	theme	taxonomic	242:250	arg1	position					252:259	The taxonomic position	238:259	The taxonomic position of the strain YIM CF22T	238:283	The taxonomic position of the strain YIM CF22T is described based on a polyphasic approach.
29974366	4	10	theme	YIM	337:339	arg1	CF22T					341:345	Strain YIM CF22T	330:345	Strain YIM CF22T	330:345	Strain YIM CF22T was found to form irregular sporangia on agar media.
29974366	6	11	theme	polar	523:527	arg1	glycolipids					679:689	two unidentified glycolipids	662:689	two unidentified glycolipids	662:689	The major menaquinone was identified as MK-9(H4); the polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, two unidentified phospholipids and two unidentified glycolipids.
29974366	6	11	theme	polar	523:527	arg1	diphosphatidylglycerol					555:576	diphosphatidylglycerol	555:576	diphosphatidylglycerol	555:576	The major menaquinone was identified as MK-9(H4); the polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, two unidentified phospholipids and two unidentified glycolipids.
29974366	6	11	theme	polar	523:527	arg1	phosphatidylethanolamine					579:602	phosphatidylethanolamine	579:602	phosphatidylethanolamine	579:602	The major menaquinone was identified as MK-9(H4); the polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, two unidentified phospholipids and two unidentified glycolipids.
29974366	6	11	theme	polar	523:527	arg1	phospholipids					644:656	two unidentified phospholipids	627:656	two unidentified phospholipids	627:656	The major menaquinone was identified as MK-9(H4); the polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, two unidentified phospholipids and two unidentified glycolipids.
29974366	6	11	theme	polar	523:527	arg1	lipids					529:534	the polar lipids	519:534	the polar lipids	519:534	The major menaquinone was identified as MK-9(H4); the polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, two unidentified phospholipids and two unidentified glycolipids.
29974366	6	11	theme	polar	523:527	arg1	phosphatidylinositol					605:624	phosphatidylinositol	605:624	phosphatidylinositol	605:624	The major menaquinone was identified as MK-9(H4); the polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, two unidentified phospholipids and two unidentified glycolipids.
29974366	2	12	theme	Uyghur	191:196	arg1	Region					209:214	Xinjiang Uyghur Autonomous Region	182:214	Xinjiang Uyghur Autonomous Region	182:214	A novel actinomycete, designated strain YIM CF22T, was isolated from a desert soil sample collected from Turpan in Xinjiang Uyghur Autonomous Region, north-western China.
29974366	2	12	theme	Uyghur	191:196	arg1	China					231:235	north-western China	217:235	north-western China	217:235	A novel actinomycete, designated strain YIM CF22T, was isolated from a desert soil sample collected from Turpan in Xinjiang Uyghur Autonomous Region, north-western China.
29974366	3	13	theme	strain	268:273	arg1	CF22T					279:283	the strain YIM CF22T	264:283	the strain YIM CF22T	264:283	The taxonomic position of the strain YIM CF22T is described based on a polyphasic approach.
29974366	13	14	theme	DNA-DNA	1550:1556	arg1	relatedness					1558:1568	DNA-DNA relatedness	1550:1568	DNA-DNA relatedness	1550:1568	Based on the differential phenotypic characteristics and the results of DNA-DNA relatedness and phylogenetic analysis, it is proposed that strain YIM CF22T represents a novel species of the genus Actinoplanes, for which the name Actinoplanes deserti sp.
29974366	4	15	theme	irregular	365:373	arg1	sporangia					375:383	irregular sporangia	365:383	irregular sporangia on agar media	365:397	Strain YIM CF22T was found to form irregular sporangia on agar media.
29974366	13	16	theme	Actinoplanes	1707:1718	arg1	sp					1728:1729	the name Actinoplanes deserti sp	1698:1729	the name Actinoplanes deserti sp	1698:1729	Based on the differential phenotypic characteristics and the results of DNA-DNA relatedness and phylogenetic analysis, it is proposed that strain YIM CF22T represents a novel species of the genus Actinoplanes, for which the name Actinoplanes deserti sp.
29974366	2	17	theme	north-western	217:229	arg1	Region					209:214	Xinjiang Uyghur Autonomous Region	182:214	Xinjiang Uyghur Autonomous Region	182:214	A novel actinomycete, designated strain YIM CF22T, was isolated from a desert soil sample collected from Turpan in Xinjiang Uyghur Autonomous Region, north-western China.
29974366	2	17	theme	north-western	217:229	arg1	China					231:235	north-western China	217:235	north-western China	217:235	A novel actinomycete, designated strain YIM CF22T, was isolated from a desert soil sample collected from Turpan in Xinjiang Uyghur Autonomous Region, north-western China.
29974366	8	18	theme	cellular	789:796	arg1	acids					804:808	The major cellular fatty acids	779:808	The major cellular fatty acids	779:808	The major cellular fatty acids were found to be (> 5%) iso-C16:0 (43.5%), anteiso-C17:0 (10.2%), iso-C15:0 (7.1%), C17:1 ω8c (6.3%) and iso H-C16:1 (5.9%).
29974366	8	18	theme	cellular	789:796	arg1	iso-C16:0					834:842	iso-C16:0	834:842	iso-C16:0	834:842	The major cellular fatty acids were found to be (> 5%) iso-C16:0 (43.5%), anteiso-C17:0 (10.2%), iso-C15:0 (7.1%), C17:1 ω8c (6.3%) and iso H-C16:1 (5.9%).
29974366	10	19	theme	CF22T	1025:1029	arg1	analysis					1002:1009	16S rRNA gene sequence analysis	979:1009	16S rRNA gene sequence analysis of strain YIM CF22T	979:1029	16S rRNA gene sequence analysis of strain YIM CF22T showed high similarity (97.0%) to Actinoplanes rishiriensis NBRC 108556T.
29974366	12	20	theme	strain	1342:1347	arg1	YIM					1349:1351	strain YIM	1342:1351	strain YIM	1342:1351	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain YIM CF22T clusters with A. rishiriensis NBRC 108556T, Actinoplanes globisporus JCM 3186T and Actinoplanes rhizophilus NEAU-A-2T.
29974366	3	21	theme	CF22T	279:283	arg1	position					252:259	The taxonomic position	238:259	The taxonomic position of the strain YIM CF22T	238:283	The taxonomic position of the strain YIM CF22T is described based on a polyphasic approach.
29974366	13	22	theme	differential	1491:1502	arg1	characteristics					1515:1529	differential phenotypic characteristics	1491:1529	differential phenotypic characteristics	1491:1529	Based on the differential phenotypic characteristics and the results of DNA-DNA relatedness and phylogenetic analysis, it is proposed that strain YIM CF22T represents a novel species of the genus Actinoplanes, for which the name Actinoplanes deserti sp.
29974366	12	23	theme	16S	1304:1306	arg1	rRNA					1308:1311	16S rRNA	1304:1311	the 16S rRNA gene sequence	1300:1325	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain YIM CF22T clusters with A. rishiriensis NBRC 108556T, Actinoplanes globisporus JCM 3186T and Actinoplanes rhizophilus NEAU-A-2T.
29974366	3	24	theme	polyphasic	309:318	arg1	approach					320:327	a polyphasic approach	307:327	a polyphasic approach	307:327	The taxonomic position of the strain YIM CF22T is described based on a polyphasic approach.
29974366	1	25	theme	desert	47:52	arg1	sample					59:64	a desert soil sample	45:64	a desert soil sample	45:64	nov., isolated from a desert soil sample.
29974366	12	26	theme	Actinoplanes	1442:1453	arg1	NEAU-A-2T					1467:1475	Actinoplanes rhizophilus NEAU-A-2T	1442:1475	Actinoplanes rhizophilus NEAU-A-2T	1442:1475	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain YIM CF22T clusters with A. rishiriensis NBRC 108556T, Actinoplanes globisporus JCM 3186T and Actinoplanes rhizophilus NEAU-A-2T.
29974366	12	27	theme	gene	1313:1316	arg1	sequence					1318:1325	the 16S rRNA gene sequence	1300:1325	the 16S rRNA gene sequence	1300:1325	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain YIM CF22T clusters with A. rishiriensis NBRC 108556T, Actinoplanes globisporus JCM 3186T and Actinoplanes rhizophilus NEAU-A-2T.
29974366	10	28	theme	rishiriensis	1078:1089	arg1	108556T					1096:1102	Actinoplanes rishiriensis NBRC 108556T	1065:1102	Actinoplanes rishiriensis NBRC 108556T	1065:1102	16S rRNA gene sequence analysis of strain YIM CF22T showed high similarity (97.0%) to Actinoplanes rishiriensis NBRC 108556T.
29974366	11	29	theme	DSM	1249:1251	arg1	45142T					1253:1258	Micromonospora tulbaghiae DSM 45142T	1223:1258	Micromonospora tulbaghiae DSM 45142T (96.8%)	1223:1266	The strain also showed high 16S rRNA gene sequence similarities to Verrucosispora sediminis CGMCC 4.3550T (96.9%) and Micromonospora tulbaghiae DSM 45142T (96.8%).
29974366	11	29	theme	DSM	1249:1251	arg1	%					1265:1265	96.8%	1261:1265	96.8%	1261:1265	The strain also showed high 16S rRNA gene sequence similarities to Verrucosispora sediminis CGMCC 4.3550T (96.9%) and Micromonospora tulbaghiae DSM 45142T (96.8%).
29974366	6	30	theme	unidentified	666:677	arg1	glycolipids					679:689	two unidentified glycolipids	662:689	two unidentified glycolipids	662:689	The major menaquinone was identified as MK-9(H4); the polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, two unidentified phospholipids and two unidentified glycolipids.
29974366	6	30	theme	unidentified	666:677	arg1	lipids					529:534	the polar lipids	519:534	the polar lipids	519:534	The major menaquinone was identified as MK-9(H4); the polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, two unidentified phospholipids and two unidentified glycolipids.
29974366	0	31	theme	Actinoplanes	0:11	arg1	sp					21:22	Actinoplanes deserti sp	0:22	Actinoplanes deserti sp.	0:23	Actinoplanes deserti sp.
29974366	6	32	theme	unidentified	631:642	arg1	lipids					529:534	the polar lipids	519:534	the polar lipids	519:534	The major menaquinone was identified as MK-9(H4); the polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, two unidentified phospholipids and two unidentified glycolipids.
29974366	6	32	theme	unidentified	631:642	arg1	phospholipids					644:656	two unidentified phospholipids	627:656	two unidentified phospholipids	627:656	The major menaquinone was identified as MK-9(H4); the polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, two unidentified phospholipids and two unidentified glycolipids.
29974366	15	33	theme	= KCTC	1780:1785	arg1	CF22T					1773:1777	YIM CF22T	1769:1777	YIM CF22T (= KCTC 39543T = CCTCC AB2018113T)	1769:1812	The type strain is YIM CF22T (= KCTC 39543T = CCTCC AB2018113T).
29974366	15	33	theme	= KCTC	1780:1785	arg1	AB2018113T					1802:1811	= KCTC 39543T = CCTCC AB2018113T	1780:1811	= KCTC 39543T = CCTCC AB2018113T	1780:1811	The type strain is YIM CF22T (= KCTC 39543T = CCTCC AB2018113T).
29974366	2	34	theme	desert	138:143	arg1	sample					150:155	a desert soil sample	136:155	a desert soil sample collected from Turpan in Xinjiang Uyghur Autonomous Region, north-western China	136:235	A novel actinomycete, designated strain YIM CF22T, was isolated from a desert soil sample collected from Turpan in Xinjiang Uyghur Autonomous Region, north-western China.
29974366	5	35	contain	contains	403:410	arg1	It					400:401	It	400:401	It	400:401	It contains meso-diaminopimelic acid in the cell wall peptidoglycan.
29974366	5	35	contain	contains	403:410	arg2	acid					432:435	meso-diaminopimelic acid	412:435	meso-diaminopimelic acid in the cell wall peptidoglycan	412:466	It contains meso-diaminopimelic acid in the cell wall peptidoglycan.
29974366	9	36	theme	G+C	939:941	arg1	content					943:949	The G+C content	935:949	The G+C content	935:949	The G+C content was determined to be 70.8%.
29974366	9	36	theme	G+C	939:941	arg1	%					976:976	70.8%	972:976	70.8%	972:976	The G+C content was determined to be 70.8%.
29974366	5	37	from	acid	432:435	arg1	peptidoglycan					454:466	the cell wall peptidoglycan	440:466	the cell wall peptidoglycan	440:466	It contains meso-diaminopimelic acid in the cell wall peptidoglycan.
29974366	2	38	attach	isolated	122:129	arg2	actinomycete					75:86	A novel actinomycete	67:86	A novel actinomycete	67:86	A novel actinomycete, designated strain YIM CF22T, was isolated from a desert soil sample collected from Turpan in Xinjiang Uyghur Autonomous Region, north-western China.
29974366	2	38	attach	isolated	122:129	arg1	sample					150:155	a desert soil sample	136:155	a desert soil sample collected from Turpan in Xinjiang Uyghur Autonomous Region, north-western China	136:235	A novel actinomycete, designated strain YIM CF22T, was isolated from a desert soil sample collected from Turpan in Xinjiang Uyghur Autonomous Region, north-western China.
29974366	10	39	theme	high	1038:1041	arg1	similarity					1043:1052	high similarity	1038:1052	high similarity (97.0%) to Actinoplanes rishiriensis NBRC 108556T	1038:1102	16S rRNA gene sequence analysis of strain YIM CF22T showed high similarity (97.0%) to Actinoplanes rishiriensis NBRC 108556T.
29974366	10	39	theme	high	1038:1041	arg1	%					1059:1059	97.0%	1055:1059	97.0%	1055:1059	16S rRNA gene sequence analysis of strain YIM CF22T showed high similarity (97.0%) to Actinoplanes rishiriensis NBRC 108556T.
29974366	10	40	theme	sequence	993:1000	arg1	analysis					1002:1009	16S rRNA gene sequence analysis	979:1009	16S rRNA gene sequence analysis of strain YIM CF22T	979:1029	16S rRNA gene sequence analysis of strain YIM CF22T showed high similarity (97.0%) to Actinoplanes rishiriensis NBRC 108556T.
29974366	12	41	theme	Phylogenetic	1269:1280	arg1	analysis					1282:1289	Phylogenetic analysis	1269:1289	Phylogenetic analysis based on the 16S rRNA gene sequence	1269:1325	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain YIM CF22T clusters with A. rishiriensis NBRC 108556T, Actinoplanes globisporus JCM 3186T and Actinoplanes rhizophilus NEAU-A-2T.
29974366	11	42	theme	sediminis	1187:1195	arg1	4.3550T					1203:1209	Verrucosispora sediminis CGMCC 4.3550T	1172:1209	Verrucosispora sediminis CGMCC 4.3550T (96.9%)	1172:1217	The strain also showed high 16S rRNA gene sequence similarities to Verrucosispora sediminis CGMCC 4.3550T (96.9%) and Micromonospora tulbaghiae DSM 45142T (96.8%).
29974366	11	42	theme	sediminis	1187:1195	arg1	%					1216:1216	96.9%	1212:1216	96.9%	1212:1216	The strain also showed high 16S rRNA gene sequence similarities to Verrucosispora sediminis CGMCC 4.3550T (96.9%) and Micromonospora tulbaghiae DSM 45142T (96.8%).
29974366	10	43	theme	rRNA	983:986	arg1	analysis					1002:1009	16S rRNA gene sequence analysis	979:1009	16S rRNA gene sequence analysis of strain YIM CF22T	979:1029	16S rRNA gene sequence analysis of strain YIM CF22T showed high similarity (97.0%) to Actinoplanes rishiriensis NBRC 108556T.
29974366	11	44	theme	high	1128:1131	arg1	similarities					1156:1167	high 16S rRNA gene sequence similarities	1128:1167	high 16S rRNA gene sequence similarities to Verrucosispora sediminis CGMCC 4.3550T (96.9%) and Micromonospora tulbaghiae DSM 45142T (96.8%)	1128:1266	The strain also showed high 16S rRNA gene sequence similarities to Verrucosispora sediminis CGMCC 4.3550T (96.9%) and Micromonospora tulbaghiae DSM 45142T (96.8%).
29974366	13	45	theme	YIM	1624:1626	arg1	CF22T					1628:1632	strain YIM CF22T	1617:1632	strain YIM CF22T	1617:1632	Based on the differential phenotypic characteristics and the results of DNA-DNA relatedness and phylogenetic analysis, it is proposed that strain YIM CF22T represents a novel species of the genus Actinoplanes, for which the name Actinoplanes deserti sp.
29974366	2	46	theme	strain	100:105	arg1	CF22T					111:115	strain YIM CF22T	100:115	strain YIM CF22T	100:115	A novel actinomycete, designated strain YIM CF22T, was isolated from a desert soil sample collected from Turpan in Xinjiang Uyghur Autonomous Region, north-western China.
29974366	10	47	theme	YIM	1021:1023	arg1	CF22T					1025:1029	strain YIM CF22T	1014:1029	strain YIM CF22T	1014:1029	16S rRNA gene sequence analysis of strain YIM CF22T showed high similarity (97.0%) to Actinoplanes rishiriensis NBRC 108556T.
29974366	15	48	theme	type	1754:1757	arg1	CF22T					1773:1777	YIM CF22T	1769:1777	YIM CF22T (= KCTC 39543T = CCTCC AB2018113T)	1769:1812	The type strain is YIM CF22T (= KCTC 39543T = CCTCC AB2018113T).
29974366	15	48	theme	type	1754:1757	arg1	strain					1759:1764	The type strain	1750:1764	The type strain	1750:1764	The type strain is YIM CF22T (= KCTC 39543T = CCTCC AB2018113T).
29974366	7	49	theme	whole	696:700	arg1	sugars					707:712	The whole cell sugars	692:712	The whole cell sugars	692:712	The whole cell sugars were found to be ribose, mannose, galactose, glucose and xylose.
29974366	7	49	theme	whole	696:700	arg1	ribose					731:736	ribose	731:736	ribose	731:736	The whole cell sugars were found to be ribose, mannose, galactose, glucose and xylose.
29974366	6	50	theme	major	473:477	arg1	menaquinone					479:489	The major menaquinone	469:489	The major menaquinone	469:489	The major menaquinone was identified as MK-9(H4); the polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, two unidentified phospholipids and two unidentified glycolipids.
29974366	6	50	theme	major	473:477	arg1	MK-9					509:512	MK-9	509:512	MK-9(H4)	509:516	The major menaquinone was identified as MK-9(H4); the polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, two unidentified phospholipids and two unidentified glycolipids.
29974366	12	51	dep	108556T	1394:1400	arg1	Actinoplanes					1403:1414	Actinoplanes	1403:1414	Actinoplanes	1403:1414	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain YIM CF22T clusters with A. rishiriensis NBRC 108556T, Actinoplanes globisporus JCM 3186T and Actinoplanes rhizophilus NEAU-A-2T.
29974366	5	52	theme	wall	449:452	arg1	peptidoglycan					454:466	the cell wall peptidoglycan	440:466	the cell wall peptidoglycan	440:466	It contains meso-diaminopimelic acid in the cell wall peptidoglycan.
29974366	4	53	theme	Strain	330:335	arg1	CF22T					341:345	Strain YIM CF22T	330:345	Strain YIM CF22T	330:345	Strain YIM CF22T was found to form irregular sporangia on agar media.
29974366	13	54	theme	relatedness	1558:1568	arg1	characteristics					1515:1529	differential phenotypic characteristics	1491:1529	differential phenotypic characteristics	1491:1529	Based on the differential phenotypic characteristics and the results of DNA-DNA relatedness and phylogenetic analysis, it is proposed that strain YIM CF22T represents a novel species of the genus Actinoplanes, for which the name Actinoplanes deserti sp.
29974366	13	54	theme	relatedness	1558:1568	arg1	results					1539:1545	the results	1535:1545	the results	1535:1545	Based on the differential phenotypic characteristics and the results of DNA-DNA relatedness and phylogenetic analysis, it is proposed that strain YIM CF22T represents a novel species of the genus Actinoplanes, for which the name Actinoplanes deserti sp.
29974366	13	55	theme	phylogenetic	1574:1585	arg1	analysis					1587:1594	phylogenetic analysis	1574:1594	phylogenetic analysis	1574:1594	Based on the differential phenotypic characteristics and the results of DNA-DNA relatedness and phylogenetic analysis, it is proposed that strain YIM CF22T represents a novel species of the genus Actinoplanes, for which the name Actinoplanes deserti sp.
29974366	13	56	theme	Actinoplanes	1674:1685	arg1	species					1653:1659	a novel species	1645:1659	a novel species	1645:1659	Based on the differential phenotypic characteristics and the results of DNA-DNA relatedness and phylogenetic analysis, it is proposed that strain YIM CF22T represents a novel species of the genus Actinoplanes, for which the name Actinoplanes deserti sp.
29974366	11	57	theme	sequence	1147:1154	arg1	similarities					1156:1167	high 16S rRNA gene sequence similarities	1128:1167	high 16S rRNA gene sequence similarities to Verrucosispora sediminis CGMCC 4.3550T (96.9%) and Micromonospora tulbaghiae DSM 45142T (96.8%)	1128:1266	The strain also showed high 16S rRNA gene sequence similarities to Verrucosispora sediminis CGMCC 4.3550T (96.9%) and Micromonospora tulbaghiae DSM 45142T (96.8%).
29974366	2	58	theme	Autonomous	198:207	arg1	Region					209:214	Xinjiang Uyghur Autonomous Region	182:214	Xinjiang Uyghur Autonomous Region	182:214	A novel actinomycete, designated strain YIM CF22T, was isolated from a desert soil sample collected from Turpan in Xinjiang Uyghur Autonomous Region, north-western China.
29974366	2	58	theme	Autonomous	198:207	arg1	China					231:235	north-western China	217:235	north-western China	217:235	A novel actinomycete, designated strain YIM CF22T, was isolated from a desert soil sample collected from Turpan in Xinjiang Uyghur Autonomous Region, north-western China.
29974366	11	59	theme	rRNA	1137:1140	arg1	similarities					1156:1167	high 16S rRNA gene sequence similarities	1128:1167	high 16S rRNA gene sequence similarities to Verrucosispora sediminis CGMCC 4.3550T (96.9%) and Micromonospora tulbaghiae DSM 45142T (96.8%)	1128:1266	The strain also showed high 16S rRNA gene sequence similarities to Verrucosispora sediminis CGMCC 4.3550T (96.9%) and Micromonospora tulbaghiae DSM 45142T (96.8%).
29974366	2	60	theme	novel	69:73	arg1	actinomycete					75:86	A novel actinomycete	67:86	A novel actinomycete	67:86	A novel actinomycete, designated strain YIM CF22T, was isolated from a desert soil sample collected from Turpan in Xinjiang Uyghur Autonomous Region, north-western China.
29974366	3	61	theme	YIM	275:277	arg1	CF22T					279:283	the strain YIM CF22T	264:283	the strain YIM CF22T	264:283	The taxonomic position of the strain YIM CF22T is described based on a polyphasic approach.
29974366	11	62	dep	Micromonospora	1223:1236	arg1	tulbaghiae					1238:1247	tulbaghiae	1238:1247	tulbaghiae	1238:1247	The strain also showed high 16S rRNA gene sequence similarities to Verrucosispora sediminis CGMCC 4.3550T (96.9%) and Micromonospora tulbaghiae DSM 45142T (96.8%).
29974366	13	63	theme	name	1702:1705	arg1	sp					1728:1729	the name Actinoplanes deserti sp	1698:1729	the name Actinoplanes deserti sp	1698:1729	Based on the differential phenotypic characteristics and the results of DNA-DNA relatedness and phylogenetic analysis, it is proposed that strain YIM CF22T represents a novel species of the genus Actinoplanes, for which the name Actinoplanes deserti sp.
29974366	4	64	from	sporangia	375:383	arg1	media					393:397	agar media	388:397	agar media	388:397	Strain YIM CF22T was found to form irregular sporangia on agar media.
29974366	4	65	theme	agar	388:391	arg1	media					393:397	agar media	388:397	agar media	388:397	Strain YIM CF22T was found to form irregular sporangia on agar media.
29974366	13	66	theme	deserti	1720:1726	arg1	sp					1728:1729	the name Actinoplanes deserti sp	1698:1729	the name Actinoplanes deserti sp	1698:1729	Based on the differential phenotypic characteristics and the results of DNA-DNA relatedness and phylogenetic analysis, it is proposed that strain YIM CF22T represents a novel species of the genus Actinoplanes, for which the name Actinoplanes deserti sp.
29974366	8	67	theme	major	783:787	arg1	acids					804:808	The major cellular fatty acids	779:808	The major cellular fatty acids	779:808	The major cellular fatty acids were found to be (> 5%) iso-C16:0 (43.5%), anteiso-C17:0 (10.2%), iso-C15:0 (7.1%), C17:1 ω8c (6.3%) and iso H-C16:1 (5.9%).
29974366	8	67	theme	major	783:787	arg1	iso-C16:0					834:842	iso-C16:0	834:842	iso-C16:0	834:842	The major cellular fatty acids were found to be (> 5%) iso-C16:0 (43.5%), anteiso-C17:0 (10.2%), iso-C15:0 (7.1%), C17:1 ω8c (6.3%) and iso H-C16:1 (5.9%).
29974366	13	68	theme	analysis	1587:1594	arg1	characteristics					1515:1529	differential phenotypic characteristics	1491:1529	differential phenotypic characteristics	1491:1529	Based on the differential phenotypic characteristics and the results of DNA-DNA relatedness and phylogenetic analysis, it is proposed that strain YIM CF22T represents a novel species of the genus Actinoplanes, for which the name Actinoplanes deserti sp.
29974366	13	68	theme	analysis	1587:1594	arg1	results					1539:1545	the results	1535:1545	the results	1535:1545	Based on the differential phenotypic characteristics and the results of DNA-DNA relatedness and phylogenetic analysis, it is proposed that strain YIM CF22T represents a novel species of the genus Actinoplanes, for which the name Actinoplanes deserti sp.
29974366	8	69	theme	fatty	798:802	arg1	acids					804:808	The major cellular fatty acids	779:808	The major cellular fatty acids	779:808	The major cellular fatty acids were found to be (> 5%) iso-C16:0 (43.5%), anteiso-C17:0 (10.2%), iso-C15:0 (7.1%), C17:1 ω8c (6.3%) and iso H-C16:1 (5.9%).
29974366	8	69	theme	fatty	798:802	arg1	iso-C16:0					834:842	iso-C16:0	834:842	iso-C16:0	834:842	The major cellular fatty acids were found to be (> 5%) iso-C16:0 (43.5%), anteiso-C17:0 (10.2%), iso-C15:0 (7.1%), C17:1 ω8c (6.3%) and iso H-C16:1 (5.9%).
29974366	12	70	theme	rRNA	1308:1311	arg1	sequence					1318:1325	the 16S rRNA gene sequence	1300:1325	the 16S rRNA gene sequence	1300:1325	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain YIM CF22T clusters with A. rishiriensis NBRC 108556T, Actinoplanes globisporus JCM 3186T and Actinoplanes rhizophilus NEAU-A-2T.
29974366	13	71	theme	phenotypic	1504:1513	arg1	characteristics					1515:1529	differential phenotypic characteristics	1491:1529	differential phenotypic characteristics	1491:1529	Based on the differential phenotypic characteristics and the results of DNA-DNA relatedness and phylogenetic analysis, it is proposed that strain YIM CF22T represents a novel species of the genus Actinoplanes, for which the name Actinoplanes deserti sp.
29974366	11	72	theme	Micromonospora	1223:1236	arg1	45142T					1253:1258	Micromonospora tulbaghiae DSM 45142T	1223:1258	Micromonospora tulbaghiae DSM 45142T (96.8%)	1223:1266	The strain also showed high 16S rRNA gene sequence similarities to Verrucosispora sediminis CGMCC 4.3550T (96.9%) and Micromonospora tulbaghiae DSM 45142T (96.8%).
29974366	11	72	theme	Micromonospora	1223:1236	arg1	%					1265:1265	96.8%	1261:1265	96.8%	1261:1265	The strain also showed high 16S rRNA gene sequence similarities to Verrucosispora sediminis CGMCC 4.3550T (96.9%) and Micromonospora tulbaghiae DSM 45142T (96.8%).
29974366	10	73	theme	NBRC	1091:1094	arg1	108556T					1096:1102	Actinoplanes rishiriensis NBRC 108556T	1065:1102	Actinoplanes rishiriensis NBRC 108556T	1065:1102	16S rRNA gene sequence analysis of strain YIM CF22T showed high similarity (97.0%) to Actinoplanes rishiriensis NBRC 108556T.
29974366	1	74	theme	soil	54:57	arg1	sample					59:64	a desert soil sample	45:64	a desert soil sample	45:64	nov., isolated from a desert soil sample.
29974366	10	75	theme	gene	988:991	arg1	analysis					1002:1009	16S rRNA gene sequence analysis	979:1009	16S rRNA gene sequence analysis of strain YIM CF22T	979:1029	16S rRNA gene sequence analysis of strain YIM CF22T showed high similarity (97.0%) to Actinoplanes rishiriensis NBRC 108556T.
29974366	13	76	theme	genus	1668:1672	arg1	Actinoplanes					1674:1685	the genus Actinoplanes	1664:1685	the genus Actinoplanes	1664:1685	Based on the differential phenotypic characteristics and the results of DNA-DNA relatedness and phylogenetic analysis, it is proposed that strain YIM CF22T represents a novel species of the genus Actinoplanes, for which the name Actinoplanes deserti sp.
29974366	0	77	theme	deserti	13:19	arg1	sp					21:22	Actinoplanes deserti sp	0:22	Actinoplanes deserti sp.	0:23	Actinoplanes deserti sp.
29974366	8	78	theme	iso	915:917	arg1	H-C16:1					919:925	iso H-C16:1	915:925	iso H-C16:1 (5.9%)	915:932	The major cellular fatty acids were found to be (> 5%) iso-C16:0 (43.5%), anteiso-C17:0 (10.2%), iso-C15:0 (7.1%), C17:1 ω8c (6.3%) and iso H-C16:1 (5.9%).
29974366	8	78	theme	iso	915:917	arg1	%					931:931	5.9%	928:931	5.9%	928:931	The major cellular fatty acids were found to be (> 5%) iso-C16:0 (43.5%), anteiso-C17:0 (10.2%), iso-C15:0 (7.1%), C17:1 ω8c (6.3%) and iso H-C16:1 (5.9%).
29974366	12	79	theme	rhizophilus	1455:1465	arg1	NEAU-A-2T					1467:1475	Actinoplanes rhizophilus NEAU-A-2T	1442:1475	Actinoplanes rhizophilus NEAU-A-2T	1442:1475	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain YIM CF22T clusters with A. rishiriensis NBRC 108556T, Actinoplanes globisporus JCM 3186T and Actinoplanes rhizophilus NEAU-A-2T.
29974366	2	80	theme	soil	145:148	arg1	sample					150:155	a desert soil sample	136:155	a desert soil sample collected from Turpan in Xinjiang Uyghur Autonomous Region, north-western China	136:235	A novel actinomycete, designated strain YIM CF22T, was isolated from a desert soil sample collected from Turpan in Xinjiang Uyghur Autonomous Region, north-western China.
29974366	13	81	theme	novel	1647:1651	arg1	species					1653:1659	a novel species	1645:1659	a novel species	1645:1659	Based on the differential phenotypic characteristics and the results of DNA-DNA relatedness and phylogenetic analysis, it is proposed that strain YIM CF22T represents a novel species of the genus Actinoplanes, for which the name Actinoplanes deserti sp.
29974366	12	82	theme	JCM	1428:1430	arg1	3186T					1432:1436	JCM 3186T	1428:1436	JCM 3186T	1428:1436	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain YIM CF22T clusters with A. rishiriensis NBRC 108556T, Actinoplanes globisporus JCM 3186T and Actinoplanes rhizophilus NEAU-A-2T.
29974366	15	83	theme	39543T = CCTCC	1787:1800	arg1	CF22T					1773:1777	YIM CF22T	1769:1777	YIM CF22T (= KCTC 39543T = CCTCC AB2018113T)	1769:1812	The type strain is YIM CF22T (= KCTC 39543T = CCTCC AB2018113T).
29974366	15	83	theme	39543T = CCTCC	1787:1800	arg1	AB2018113T					1802:1811	= KCTC 39543T = CCTCC AB2018113T	1780:1811	= KCTC 39543T = CCTCC AB2018113T	1780:1811	The type strain is YIM CF22T (= KCTC 39543T = CCTCC AB2018113T).
29974366	12	84	dep	3186T	1432:1436	arg1	globisporus					1416:1426	globisporus	1416:1426	globisporus	1416:1426	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain YIM CF22T clusters with A. rishiriensis NBRC 108556T, Actinoplanes globisporus JCM 3186T and Actinoplanes rhizophilus NEAU-A-2T.
29974366	11	85	theme	CGMCC	1197:1201	arg1	4.3550T					1203:1209	Verrucosispora sediminis CGMCC 4.3550T	1172:1209	Verrucosispora sediminis CGMCC 4.3550T (96.9%)	1172:1217	The strain also showed high 16S rRNA gene sequence similarities to Verrucosispora sediminis CGMCC 4.3550T (96.9%) and Micromonospora tulbaghiae DSM 45142T (96.8%).
29974366	11	85	theme	CGMCC	1197:1201	arg1	%					1216:1216	96.9%	1212:1216	96.9%	1212:1216	The strain also showed high 16S rRNA gene sequence similarities to Verrucosispora sediminis CGMCC 4.3550T (96.9%) and Micromonospora tulbaghiae DSM 45142T (96.8%).
29974366	15	86	theme	YIM	1769:1771	arg1	CF22T					1773:1777	YIM CF22T	1769:1777	YIM CF22T (= KCTC 39543T = CCTCC AB2018113T)	1769:1812	The type strain is YIM CF22T (= KCTC 39543T = CCTCC AB2018113T).
29974366	15	86	theme	YIM	1769:1771	arg1	AB2018113T					1802:1811	= KCTC 39543T = CCTCC AB2018113T	1780:1811	= KCTC 39543T = CCTCC AB2018113T	1780:1811	The type strain is YIM CF22T (= KCTC 39543T = CCTCC AB2018113T).
29974366	15	86	theme	YIM	1769:1771	arg1	strain					1759:1764	The type strain	1750:1764	The type strain	1750:1764	The type strain is YIM CF22T (= KCTC 39543T = CCTCC AB2018113T).
29974366	10	87	theme	Actinoplanes	1065:1076	arg1	108556T					1096:1102	Actinoplanes rishiriensis NBRC 108556T	1065:1102	Actinoplanes rishiriensis NBRC 108556T	1065:1102	16S rRNA gene sequence analysis of strain YIM CF22T showed high similarity (97.0%) to Actinoplanes rishiriensis NBRC 108556T.
29974366	13	88	theme	strain	1617:1622	arg1	CF22T					1628:1632	strain YIM CF22T	1617:1632	strain YIM CF22T	1617:1632	Based on the differential phenotypic characteristics and the results of DNA-DNA relatedness and phylogenetic analysis, it is proposed that strain YIM CF22T represents a novel species of the genus Actinoplanes, for which the name Actinoplanes deserti sp.
29974366	2	89	theme	YIM	107:109	arg1	CF22T					111:115	strain YIM CF22T	100:115	strain YIM CF22T	100:115	A novel actinomycete, designated strain YIM CF22T, was isolated from a desert soil sample collected from Turpan in Xinjiang Uyghur Autonomous Region, north-western China.
29974366	10	90	theme	16S	979:981	arg1	analysis					1002:1009	16S rRNA gene sequence analysis	979:1009	16S rRNA gene sequence analysis of strain YIM CF22T	979:1029	16S rRNA gene sequence analysis of strain YIM CF22T showed high similarity (97.0%) to Actinoplanes rishiriensis NBRC 108556T.
30599948	6	0	theme	most	1195:1198	arg1	compounds					1212:1220	the most represented compounds	1191:1220	the most represented compounds among flavonoids	1191:1237	In particular, glycosidic forms of quercetin (i.e., quercetin 3-O-galactoside, quercetin 3-O-glucoside, and quercetin 4'-O-glucoside) were the most represented compounds among flavonoids.
30599948	6	0	theme	most	1195:1198	arg1	forms					1078:1082	glycosidic forms	1067:1082	glycosidic forms of quercetin (i.e., quercetin 3-O-galactoside, quercetin 3-O-glucoside, and quercetin 4'-O-glucoside)	1067:1184	In particular, glycosidic forms of quercetin (i.e., quercetin 3-O-galactoside, quercetin 3-O-glucoside, and quercetin 4'-O-glucoside) were the most represented compounds among flavonoids.
30599948	2	1	theme	alcoholic	406:414	arg1	extracts					479:486	alcoholic (methanol 100%) and hydroalcoholic (methanol/water 50:50, v/v) extracts	406:486	alcoholic (methanol 100%) and hydroalcoholic (methanol/water 50:50, v/v) extracts	406:486	The phenolic composition of alcoholic (methanol 100%) and hydroalcoholic (methanol/water 50:50, v/v) extracts was compared by using an untargeted metabolomics-based profiling approach followed by multivariate statistics.
30599948	9	2	theme	oxygen	1763:1768	arg1	capacity					1789:1796	oxygen radical absorbance capacity	1763:1796	the highest oxygen radical absorbance capacity (536.27 μmol Trolox Equivalents/g)	1751:1831	Homogenizer-assisted extraction of M. oleifera leaves using 100% methanol allowed extracting the highest amounts of polyphenols (35.19 mg/g) and produced the highest oxygen radical absorbance capacity (536.27 μmol Trolox Equivalents/g).
30599948	9	2	theme	oxygen	1763:1768	arg1	Equivalents/g					1818:1830	536.27 μmol Trolox Equivalents/g	1799:1830	536.27 μmol Trolox Equivalents/g	1799:1830	Homogenizer-assisted extraction of M. oleifera leaves using 100% methanol allowed extracting the highest amounts of polyphenols (35.19 mg/g) and produced the highest oxygen radical absorbance capacity (536.27 μmol Trolox Equivalents/g).
30599948	4	3	theme	radical	936:942	arg1	ORAC					965:968	ORAC	965:968	ORAC	965:968	Besides, the in vitro antioxidant activities of Moringa leaves were also investigated as ferric reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC).
30599948	4	3	theme	radical	936:942	arg1	activities					829:838	the in vitro antioxidant activities	804:838	the in vitro antioxidant activities of Moringa leaves	804:856	Besides, the in vitro antioxidant activities of Moringa leaves were also investigated as ferric reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC).
30599948	4	3	theme	radical	936:942	arg1	capacity					955:962	oxygen radical absorbance capacity	929:962	oxygen radical absorbance capacity (ORAC)	929:969	Besides, the in vitro antioxidant activities of Moringa leaves were also investigated as ferric reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC).
30599948	1	4	theme	homogenizer-assisted	185:204	arg1	extraction					206:215	homogenizer-assisted extraction	185:215	homogenizer-assisted extraction	185:215	The impact of different extraction methods, namely maceration, homogenizer-assisted extraction, rapid solid-liquid dynamic extraction, microwave-assisted extraction and ultrasound-assisted extraction, on polyphenols of Moringa oleifera leaves was studied.
30599948	1	4	theme	homogenizer-assisted	185:204	arg1	methods					157:163	different extraction methods	136:163	different extraction methods	136:163	The impact of different extraction methods, namely maceration, homogenizer-assisted extraction, rapid solid-liquid dynamic extraction, microwave-assisted extraction and ultrasound-assisted extraction, on polyphenols of Moringa oleifera leaves was studied.
30599948	10	5	theme	phenolic	1925:1932	arg1	class					1956:1960	the phenolic class	1943:1960	the phenolic class mostly affected by the different extraction technologies	1943:2017	The supervised orthogonal projection to latent structures discriminant analysis identified phenolic acids as the phenolic class mostly affected by the different extraction technologies.
30599948	10	5	theme	phenolic	1925:1932	arg1	acids					1934:1938	phenolic acids	1925:1938	phenolic acids	1925:1938	The supervised orthogonal projection to latent structures discriminant analysis identified phenolic acids as the phenolic class mostly affected by the different extraction technologies.
30599948	6	6	theme	glycosidic	1067:1076	arg1	compounds					1212:1220	the most represented compounds	1191:1220	the most represented compounds among flavonoids	1191:1237	In particular, glycosidic forms of quercetin (i.e., quercetin 3-O-galactoside, quercetin 3-O-glucoside, and quercetin 4'-O-glucoside) were the most represented compounds among flavonoids.
30599948	6	6	theme	glycosidic	1067:1076	arg1	forms					1078:1082	glycosidic forms	1067:1082	glycosidic forms of quercetin (i.e., quercetin 3-O-galactoside, quercetin 3-O-glucoside, and quercetin 4'-O-glucoside)	1067:1184	In particular, glycosidic forms of quercetin (i.e., quercetin 3-O-galactoside, quercetin 3-O-glucoside, and quercetin 4'-O-glucoside) were the most represented compounds among flavonoids.
30599948	9	7	theme	Trolox	1811:1816	arg1	capacity					1789:1796	oxygen radical absorbance capacity	1763:1796	the highest oxygen radical absorbance capacity (536.27 μmol Trolox Equivalents/g)	1751:1831	Homogenizer-assisted extraction of M. oleifera leaves using 100% methanol allowed extracting the highest amounts of polyphenols (35.19 mg/g) and produced the highest oxygen radical absorbance capacity (536.27 μmol Trolox Equivalents/g).
30599948	9	7	theme	Trolox	1811:1816	arg1	Equivalents/g					1818:1830	536.27 μmol Trolox Equivalents/g	1799:1830	536.27 μmol Trolox Equivalents/g	1799:1830	Homogenizer-assisted extraction of M. oleifera leaves using 100% methanol allowed extracting the highest amounts of polyphenols (35.19 mg/g) and produced the highest oxygen radical absorbance capacity (536.27 μmol Trolox Equivalents/g).
30599948	11	8	with	recovery	2088:2095	arg1	efficiencies					2144:2155	different efficiencies	2134:2155	different efficiencies	2134:2155	These findings demonstrate that each extraction method promoted the recovery of specific phenolic subclasses with different efficiencies.
30599948	3	9	theme	ultra-high-pressure	614:632	arg1	chromatography					641:654	ultra-high-pressure liquid chromatography	614:654	ultra-high-pressure liquid chromatography coupled to a quadrupole-time-of-flight mass spectrometry	614:711	With this aim, ultra-high-pressure liquid chromatography coupled to a quadrupole-time-of-flight mass spectrometry was used to profile phenolic compounds under the different extraction conditions.
30599948	1	10	theme	solid-liquid	224:235	arg1	methods					157:163	different extraction methods	136:163	different extraction methods	136:163	The impact of different extraction methods, namely maceration, homogenizer-assisted extraction, rapid solid-liquid dynamic extraction, microwave-assisted extraction and ultrasound-assisted extraction, on polyphenols of Moringa oleifera leaves was studied.
30599948	1	10	theme	solid-liquid	224:235	arg1	extraction					245:254	rapid solid-liquid dynamic extraction	218:254	rapid solid-liquid dynamic extraction	218:254	The impact of different extraction methods, namely maceration, homogenizer-assisted extraction, rapid solid-liquid dynamic extraction, microwave-assisted extraction and ultrasound-assisted extraction, on polyphenols of Moringa oleifera leaves was studied.
30599948	3	11	theme	quadrupole-time-of-flight	669:693	arg1	spectrometry					700:711	a quadrupole-time-of-flight mass spectrometry	667:711	a quadrupole-time-of-flight mass spectrometry	667:711	With this aim, ultra-high-pressure liquid chromatography coupled to a quadrupole-time-of-flight mass spectrometry was used to profile phenolic compounds under the different extraction conditions.
30599948	4	12	theme	in	808:809	arg1	capacity					955:962	oxygen radical absorbance capacity	929:962	oxygen radical absorbance capacity (ORAC)	929:969	Besides, the in vitro antioxidant activities of Moringa leaves were also investigated as ferric reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC).
30599948	4	12	theme	in	808:809	arg1	activities					829:838	the in vitro antioxidant activities	804:838	the in vitro antioxidant activities of Moringa leaves	804:856	Besides, the in vitro antioxidant activities of Moringa leaves were also investigated as ferric reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC).
30599948	4	12	theme	in	808:809	arg1	power					912:916	ferric reducing antioxidant power	884:916	ferric reducing antioxidant power (FRAP)	884:923	Besides, the in vitro antioxidant activities of Moringa leaves were also investigated as ferric reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC).
30599948	11	13	theme	subclasses	2118:2127	arg1	recovery					2088:2095	the recovery	2084:2095	the recovery of specific phenolic subclasses with different efficiencies	2084:2155	These findings demonstrate that each extraction method promoted the recovery of specific phenolic subclasses with different efficiencies.
30599948	4	14	dep	Moringa	843:849	arg1	leaves					851:856	leaves	851:856	leaves	851:856	Besides, the in vitro antioxidant activities of Moringa leaves were also investigated as ferric reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC).
30599948	4	15	theme	antioxidant	817:827	arg1	capacity					955:962	oxygen radical absorbance capacity	929:962	oxygen radical absorbance capacity (ORAC)	929:969	Besides, the in vitro antioxidant activities of Moringa leaves were also investigated as ferric reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC).
30599948	4	15	theme	antioxidant	817:827	arg1	activities					829:838	the in vitro antioxidant activities	804:838	the in vitro antioxidant activities of Moringa leaves	804:856	Besides, the in vitro antioxidant activities of Moringa leaves were also investigated as ferric reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC).
30599948	4	15	theme	antioxidant	817:827	arg1	power					912:916	ferric reducing antioxidant power	884:916	ferric reducing antioxidant power (FRAP)	884:923	Besides, the in vitro antioxidant activities of Moringa leaves were also investigated as ferric reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC).
30599948	1	16	theme	extraction	146:155	arg1	extraction					206:215	homogenizer-assisted extraction	185:215	homogenizer-assisted extraction	185:215	The impact of different extraction methods, namely maceration, homogenizer-assisted extraction, rapid solid-liquid dynamic extraction, microwave-assisted extraction and ultrasound-assisted extraction, on polyphenols of Moringa oleifera leaves was studied.
30599948	1	16	theme	extraction	146:155	arg1	methods					157:163	different extraction methods	136:163	different extraction methods	136:163	The impact of different extraction methods, namely maceration, homogenizer-assisted extraction, rapid solid-liquid dynamic extraction, microwave-assisted extraction and ultrasound-assisted extraction, on polyphenols of Moringa oleifera leaves was studied.
30599948	1	16	theme	extraction	146:155	arg1	extraction					276:285	microwave-assisted extraction	257:285	microwave-assisted extraction	257:285	The impact of different extraction methods, namely maceration, homogenizer-assisted extraction, rapid solid-liquid dynamic extraction, microwave-assisted extraction and ultrasound-assisted extraction, on polyphenols of Moringa oleifera leaves was studied.
30599948	1	16	theme	extraction	146:155	arg1	maceration					173:182	maceration	173:182	maceration	173:182	The impact of different extraction methods, namely maceration, homogenizer-assisted extraction, rapid solid-liquid dynamic extraction, microwave-assisted extraction and ultrasound-assisted extraction, on polyphenols of Moringa oleifera leaves was studied.
30599948	1	16	theme	extraction	146:155	arg1	extraction					245:254	rapid solid-liquid dynamic extraction	218:254	rapid solid-liquid dynamic extraction	218:254	The impact of different extraction methods, namely maceration, homogenizer-assisted extraction, rapid solid-liquid dynamic extraction, microwave-assisted extraction and ultrasound-assisted extraction, on polyphenols of Moringa oleifera leaves was studied.
30599948	1	16	theme	extraction	146:155	arg1	extraction					311:320	ultrasound-assisted extraction	291:320	ultrasound-assisted extraction	291:320	The impact of different extraction methods, namely maceration, homogenizer-assisted extraction, rapid solid-liquid dynamic extraction, microwave-assisted extraction and ultrasound-assisted extraction, on polyphenols of Moringa oleifera leaves was studied.
30599948	1	17	theme	different	136:144	arg1	extraction					206:215	homogenizer-assisted extraction	185:215	homogenizer-assisted extraction	185:215	The impact of different extraction methods, namely maceration, homogenizer-assisted extraction, rapid solid-liquid dynamic extraction, microwave-assisted extraction and ultrasound-assisted extraction, on polyphenols of Moringa oleifera leaves was studied.
30599948	1	17	theme	different	136:144	arg1	methods					157:163	different extraction methods	136:163	different extraction methods	136:163	The impact of different extraction methods, namely maceration, homogenizer-assisted extraction, rapid solid-liquid dynamic extraction, microwave-assisted extraction and ultrasound-assisted extraction, on polyphenols of Moringa oleifera leaves was studied.
30599948	1	17	theme	different	136:144	arg1	extraction					276:285	microwave-assisted extraction	257:285	microwave-assisted extraction	257:285	The impact of different extraction methods, namely maceration, homogenizer-assisted extraction, rapid solid-liquid dynamic extraction, microwave-assisted extraction and ultrasound-assisted extraction, on polyphenols of Moringa oleifera leaves was studied.
30599948	1	17	theme	different	136:144	arg1	maceration					173:182	maceration	173:182	maceration	173:182	The impact of different extraction methods, namely maceration, homogenizer-assisted extraction, rapid solid-liquid dynamic extraction, microwave-assisted extraction and ultrasound-assisted extraction, on polyphenols of Moringa oleifera leaves was studied.
30599948	1	17	theme	different	136:144	arg1	extraction					245:254	rapid solid-liquid dynamic extraction	218:254	rapid solid-liquid dynamic extraction	218:254	The impact of different extraction methods, namely maceration, homogenizer-assisted extraction, rapid solid-liquid dynamic extraction, microwave-assisted extraction and ultrasound-assisted extraction, on polyphenols of Moringa oleifera leaves was studied.
30599948	1	17	theme	different	136:144	arg1	extraction					311:320	ultrasound-assisted extraction	291:320	ultrasound-assisted extraction	291:320	The impact of different extraction methods, namely maceration, homogenizer-assisted extraction, rapid solid-liquid dynamic extraction, microwave-assisted extraction and ultrasound-assisted extraction, on polyphenols of Moringa oleifera leaves was studied.
30599948	0	18	theme	phenolic	77:84	arg1	profile					86:92	the untargeted phenolic profile	62:92	the untargeted phenolic profile of Moringa oleifera	62:112	Impact of conventional/non-conventional extraction methods on the untargeted phenolic profile of Moringa oleifera leaves.
30599948	5	19	theme	metabolomic	976:986	arg1	approach					988:995	The metabolomic approach	972:995	The metabolomic approach	972:995	The metabolomic approach allowed to putatively annotate 262 phenolic compounds.
30599948	2	20	theme	metabolomics-based	524:541	arg1	approach					553:560	an untargeted metabolomics-based profiling approach	510:560	an untargeted metabolomics-based profiling approach followed by multivariate statistics	510:596	The phenolic composition of alcoholic (methanol 100%) and hydroalcoholic (methanol/water 50:50, v/v) extracts was compared by using an untargeted metabolomics-based profiling approach followed by multivariate statistics.
30599948	3	21	used	used	717:720	arg2	chromatography					641:654	ultra-high-pressure liquid chromatography	614:654	ultra-high-pressure liquid chromatography coupled to a quadrupole-time-of-flight mass spectrometry	614:711	With this aim, ultra-high-pressure liquid chromatography coupled to a quadrupole-time-of-flight mass spectrometry was used to profile phenolic compounds under the different extraction conditions.
30599948	0	22	from	Impact	0:5	arg1	profile					86:92	the untargeted phenolic profile	62:92	the untargeted phenolic profile of Moringa oleifera	62:112	Impact of conventional/non-conventional extraction methods on the untargeted phenolic profile of Moringa oleifera leaves.
30599948	0	23	theme	oleifera	105:112	arg1	profile					86:92	the untargeted phenolic profile	62:92	the untargeted phenolic profile of Moringa oleifera	62:112	Impact of conventional/non-conventional extraction methods on the untargeted phenolic profile of Moringa oleifera leaves.
30599948	9	24	theme	100	1657:1659	arg1	%					1660:1660	%	1660:1660	%	1660:1660	Homogenizer-assisted extraction of M. oleifera leaves using 100% methanol allowed extracting the highest amounts of polyphenols (35.19 mg/g) and produced the highest oxygen radical absorbance capacity (536.27 μmol Trolox Equivalents/g).
30599948	2	25	theme	hydroalcoholic	436:449	arg1	extracts					479:486	alcoholic (methanol 100%) and hydroalcoholic (methanol/water 50:50, v/v) extracts	406:486	alcoholic (methanol 100%) and hydroalcoholic (methanol/water 50:50, v/v) extracts	406:486	The phenolic composition of alcoholic (methanol 100%) and hydroalcoholic (methanol/water 50:50, v/v) extracts was compared by using an untargeted metabolomics-based profiling approach followed by multivariate statistics.
30599948	7	26	theme	abundant	1298:1305	arg1	acid					1268:1271	protocatechuic acid	1253:1271	protocatechuic acid	1253:1271	Furthermore, protocatechuic acid was found to be the most abundant hydroxybenzaldheyde derivative, while the isomeric forms of hydroxybenzoic acid characterized the phenolic acids class.
30599948	7	26	theme	abundant	1298:1305	arg1	derivative					1327:1336	the most abundant hydroxybenzaldheyde derivative	1289:1336	the most abundant hydroxybenzaldheyde derivative	1289:1336	Furthermore, protocatechuic acid was found to be the most abundant hydroxybenzaldheyde derivative, while the isomeric forms of hydroxybenzoic acid characterized the phenolic acids class.
30599948	6	27	dep	3-O-galactoside	1114:1128	arg1	i.e.					1098:1101	i.e.	1098:1101	i.e.	1098:1101	In particular, glycosidic forms of quercetin (i.e., quercetin 3-O-galactoside, quercetin 3-O-glucoside, and quercetin 4'-O-glucoside) were the most represented compounds among flavonoids.
30599948	9	28	dep	oleifera	1635:1642	arg1	leaves					1644:1649	leaves	1644:1649	leaves using 100% methanol	1644:1669	Homogenizer-assisted extraction of M. oleifera leaves using 100% methanol allowed extracting the highest amounts of polyphenols (35.19 mg/g) and produced the highest oxygen radical absorbance capacity (536.27 μmol Trolox Equivalents/g).
30599948	1	29	from	impact	126:131	arg1	polyphenols					326:336	polyphenols	326:336	polyphenols of Moringa oleifera	326:356	The impact of different extraction methods, namely maceration, homogenizer-assisted extraction, rapid solid-liquid dynamic extraction, microwave-assisted extraction and ultrasound-assisted extraction, on polyphenols of Moringa oleifera leaves was studied.
30599948	9	30	theme	absorbance	1778:1787	arg1	capacity					1789:1796	oxygen radical absorbance capacity	1763:1796	the highest oxygen radical absorbance capacity (536.27 μmol Trolox Equivalents/g)	1751:1831	Homogenizer-assisted extraction of M. oleifera leaves using 100% methanol allowed extracting the highest amounts of polyphenols (35.19 mg/g) and produced the highest oxygen radical absorbance capacity (536.27 μmol Trolox Equivalents/g).
30599948	9	30	theme	absorbance	1778:1787	arg1	Equivalents/g					1818:1830	536.27 μmol Trolox Equivalents/g	1799:1830	536.27 μmol Trolox Equivalents/g	1799:1830	Homogenizer-assisted extraction of M. oleifera leaves using 100% methanol allowed extracting the highest amounts of polyphenols (35.19 mg/g) and produced the highest oxygen radical absorbance capacity (536.27 μmol Trolox Equivalents/g).
30599948	6	31	theme	quercetin	1160:1168	arg1	4'-O-glucoside					1170:1183	quercetin 4'-O-glucoside	1160:1183	quercetin 4'-O-glucoside	1160:1183	In particular, glycosidic forms of quercetin (i.e., quercetin 3-O-galactoside, quercetin 3-O-glucoside, and quercetin 4'-O-glucoside) were the most represented compounds among flavonoids.
30599948	1	32	dep	methods	157:163	arg1	extraction					206:215	homogenizer-assisted extraction	185:215	homogenizer-assisted extraction	185:215	The impact of different extraction methods, namely maceration, homogenizer-assisted extraction, rapid solid-liquid dynamic extraction, microwave-assisted extraction and ultrasound-assisted extraction, on polyphenols of Moringa oleifera leaves was studied.
30599948	1	32	dep	methods	157:163	arg1	methods					157:163	different extraction methods	136:163	different extraction methods	136:163	The impact of different extraction methods, namely maceration, homogenizer-assisted extraction, rapid solid-liquid dynamic extraction, microwave-assisted extraction and ultrasound-assisted extraction, on polyphenols of Moringa oleifera leaves was studied.
30599948	1	32	dep	methods	157:163	arg1	extraction					276:285	microwave-assisted extraction	257:285	microwave-assisted extraction	257:285	The impact of different extraction methods, namely maceration, homogenizer-assisted extraction, rapid solid-liquid dynamic extraction, microwave-assisted extraction and ultrasound-assisted extraction, on polyphenols of Moringa oleifera leaves was studied.
30599948	1	32	dep	methods	157:163	arg1	maceration					173:182	maceration	173:182	maceration	173:182	The impact of different extraction methods, namely maceration, homogenizer-assisted extraction, rapid solid-liquid dynamic extraction, microwave-assisted extraction and ultrasound-assisted extraction, on polyphenols of Moringa oleifera leaves was studied.
30599948	1	32	dep	methods	157:163	arg1	extraction					245:254	rapid solid-liquid dynamic extraction	218:254	rapid solid-liquid dynamic extraction	218:254	The impact of different extraction methods, namely maceration, homogenizer-assisted extraction, rapid solid-liquid dynamic extraction, microwave-assisted extraction and ultrasound-assisted extraction, on polyphenols of Moringa oleifera leaves was studied.
30599948	1	32	dep	methods	157:163	arg1	extraction					311:320	ultrasound-assisted extraction	291:320	ultrasound-assisted extraction	291:320	The impact of different extraction methods, namely maceration, homogenizer-assisted extraction, rapid solid-liquid dynamic extraction, microwave-assisted extraction and ultrasound-assisted extraction, on polyphenols of Moringa oleifera leaves was studied.
30599948	0	33	theme	conventional/non-conventional	10:38	arg1	methods					51:57	conventional/non-conventional extraction methods	10:57	conventional/non-conventional extraction methods	10:57	Impact of conventional/non-conventional extraction methods on the untargeted phenolic profile of Moringa oleifera leaves.
30599948	1	34	theme	Moringa	341:347	arg1	oleifera					349:356	Moringa oleifera	341:356	Moringa oleifera	341:356	The impact of different extraction methods, namely maceration, homogenizer-assisted extraction, rapid solid-liquid dynamic extraction, microwave-assisted extraction and ultrasound-assisted extraction, on polyphenols of Moringa oleifera leaves was studied.
30599948	9	35	theme	highest	1694:1700	arg1	polyphenols					1713:1723	polyphenols	1713:1723	polyphenols (35.19 mg/g)	1713:1736	Homogenizer-assisted extraction of M. oleifera leaves using 100% methanol allowed extracting the highest amounts of polyphenols (35.19 mg/g) and produced the highest oxygen radical absorbance capacity (536.27 μmol Trolox Equivalents/g).
30599948	9	35	theme	highest	1694:1700	arg1	amounts					1702:1708	the highest amounts	1690:1708	the highest amounts of polyphenols (35.19 mg/g)	1690:1736	Homogenizer-assisted extraction of M. oleifera leaves using 100% methanol allowed extracting the highest amounts of polyphenols (35.19 mg/g) and produced the highest oxygen radical absorbance capacity (536.27 μmol Trolox Equivalents/g).
30599948	9	35	theme	highest	1694:1700	arg1	35.19 mg/g					1726:1735	35.19 mg/g	1726:1735	35.19 mg/g	1726:1735	Homogenizer-assisted extraction of M. oleifera leaves using 100% methanol allowed extracting the highest amounts of polyphenols (35.19 mg/g) and produced the highest oxygen radical absorbance capacity (536.27 μmol Trolox Equivalents/g).
30599948	2	36	dep	methanol/water	452:465	arg1	v/v					474:476	v/v	474:476	v/v	474:476	The phenolic composition of alcoholic (methanol 100%) and hydroalcoholic (methanol/water 50:50, v/v) extracts was compared by using an untargeted metabolomics-based profiling approach followed by multivariate statistics.
30599948	0	37	theme	methods	51:57	arg1	Impact					0:5	Impact	0:5	Impact of conventional/non-conventional extraction methods on the untargeted phenolic profile of Moringa oleifera	0:112	Impact of conventional/non-conventional extraction methods on the untargeted phenolic profile of Moringa oleifera leaves.
30599948	8	38	dep	methanol/water	1568:1581	arg1	50:50					1584:1588	50:50	1584:1588	50:50	1584:1588	Overall, the extractions in methanol 100% were found to be the most effective for phenolic compounds recovering, when compared with those in methanol/water (50:50, v/v).
30599948	8	38	dep	methanol/water	1568:1581	arg1	v/v					1591:1593	v/v	1591:1593	v/v	1591:1593	Overall, the extractions in methanol 100% were found to be the most effective for phenolic compounds recovering, when compared with those in methanol/water (50:50, v/v).
30599948	6	39	theme	quercetin	1131:1139	arg1	3-O-glucoside					1141:1153	quercetin 3-O-glucoside	1131:1153	quercetin 3-O-glucoside	1131:1153	In particular, glycosidic forms of quercetin (i.e., quercetin 3-O-galactoside, quercetin 3-O-glucoside, and quercetin 4'-O-glucoside) were the most represented compounds among flavonoids.
30599948	7	40	theme	isomeric	1349:1356	arg1	forms					1358:1362	the isomeric forms	1345:1362	the isomeric forms of hydroxybenzoic acid	1345:1385	Furthermore, protocatechuic acid was found to be the most abundant hydroxybenzaldheyde derivative, while the isomeric forms of hydroxybenzoic acid characterized the phenolic acids class.
30599948	7	41	theme	protocatechuic	1253:1266	arg1	acid					1268:1271	protocatechuic acid	1253:1271	protocatechuic acid	1253:1271	Furthermore, protocatechuic acid was found to be the most abundant hydroxybenzaldheyde derivative, while the isomeric forms of hydroxybenzoic acid characterized the phenolic acids class.
30599948	7	41	theme	protocatechuic	1253:1266	arg1	derivative					1327:1336	the most abundant hydroxybenzaldheyde derivative	1289:1336	the most abundant hydroxybenzaldheyde derivative	1289:1336	Furthermore, protocatechuic acid was found to be the most abundant hydroxybenzaldheyde derivative, while the isomeric forms of hydroxybenzoic acid characterized the phenolic acids class.
30599948	7	42	theme	acids	1414:1418	arg1	class					1420:1424	the phenolic acids class	1401:1424	the phenolic acids class	1401:1424	Furthermore, protocatechuic acid was found to be the most abundant hydroxybenzaldheyde derivative, while the isomeric forms of hydroxybenzoic acid characterized the phenolic acids class.
30599948	3	43	theme	extraction	772:781	arg1	conditions					783:792	the different extraction conditions	758:792	the different extraction conditions	758:792	With this aim, ultra-high-pressure liquid chromatography coupled to a quadrupole-time-of-flight mass spectrometry was used to profile phenolic compounds under the different extraction conditions.
30599948	4	44	theme	reducing	891:898	arg1	FRAP					919:922	FRAP	919:922	FRAP	919:922	Besides, the in vitro antioxidant activities of Moringa leaves were also investigated as ferric reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC).
30599948	4	44	theme	reducing	891:898	arg1	activities					829:838	the in vitro antioxidant activities	804:838	the in vitro antioxidant activities of Moringa leaves	804:856	Besides, the in vitro antioxidant activities of Moringa leaves were also investigated as ferric reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC).
30599948	4	44	theme	reducing	891:898	arg1	power					912:916	ferric reducing antioxidant power	884:916	ferric reducing antioxidant power (FRAP)	884:923	Besides, the in vitro antioxidant activities of Moringa leaves were also investigated as ferric reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC).
30599948	2	45	theme	phenolic	382:389	arg1	composition					391:401	The phenolic composition	378:401	The phenolic composition of alcoholic (methanol 100%) and hydroalcoholic (methanol/water 50:50, v/v) extracts	378:486	The phenolic composition of alcoholic (methanol 100%) and hydroalcoholic (methanol/water 50:50, v/v) extracts was compared by using an untargeted metabolomics-based profiling approach followed by multivariate statistics.
30599948	10	46	theme	projection	1860:1869	arg1	analysis					1905:1912	The supervised orthogonal projection to latent structures discriminant analysis	1834:1912	The supervised orthogonal projection to latent structures discriminant analysis	1834:1912	The supervised orthogonal projection to latent structures discriminant analysis identified phenolic acids as the phenolic class mostly affected by the different extraction technologies.
30599948	7	47	theme	acid	1382:1385	arg1	forms					1358:1362	the isomeric forms	1345:1362	the isomeric forms of hydroxybenzoic acid	1345:1385	Furthermore, protocatechuic acid was found to be the most abundant hydroxybenzaldheyde derivative, while the isomeric forms of hydroxybenzoic acid characterized the phenolic acids class.
30599948	10	48	theme	supervised	1838:1847	arg1	analysis					1905:1912	The supervised orthogonal projection to latent structures discriminant analysis	1834:1912	The supervised orthogonal projection to latent structures discriminant analysis	1834:1912	The supervised orthogonal projection to latent structures discriminant analysis identified phenolic acids as the phenolic class mostly affected by the different extraction technologies.
30599948	1	49	theme	ultrasound-assisted	291:309	arg1	extraction					311:320	ultrasound-assisted extraction	291:320	ultrasound-assisted extraction	291:320	The impact of different extraction methods, namely maceration, homogenizer-assisted extraction, rapid solid-liquid dynamic extraction, microwave-assisted extraction and ultrasound-assisted extraction, on polyphenols of Moringa oleifera leaves was studied.
30599948	1	49	theme	ultrasound-assisted	291:309	arg1	methods					157:163	different extraction methods	136:163	different extraction methods	136:163	The impact of different extraction methods, namely maceration, homogenizer-assisted extraction, rapid solid-liquid dynamic extraction, microwave-assisted extraction and ultrasound-assisted extraction, on polyphenols of Moringa oleifera leaves was studied.
30599948	9	50	theme	highest	1755:1761	arg1	capacity					1789:1796	oxygen radical absorbance capacity	1763:1796	the highest oxygen radical absorbance capacity (536.27 μmol Trolox Equivalents/g)	1751:1831	Homogenizer-assisted extraction of M. oleifera leaves using 100% methanol allowed extracting the highest amounts of polyphenols (35.19 mg/g) and produced the highest oxygen radical absorbance capacity (536.27 μmol Trolox Equivalents/g).
30599948	9	50	theme	highest	1755:1761	arg1	Equivalents/g					1818:1830	536.27 μmol Trolox Equivalents/g	1799:1830	536.27 μmol Trolox Equivalents/g	1799:1830	Homogenizer-assisted extraction of M. oleifera leaves using 100% methanol allowed extracting the highest amounts of polyphenols (35.19 mg/g) and produced the highest oxygen radical absorbance capacity (536.27 μmol Trolox Equivalents/g).
30599948	10	51	theme	different	1985:1993	arg1	technologies					2006:2017	the different extraction technologies	1981:2017	the different extraction technologies	1981:2017	The supervised orthogonal projection to latent structures discriminant analysis identified phenolic acids as the phenolic class mostly affected by the different extraction technologies.
30599948	10	52	theme	discriminant	1892:1903	arg1	analysis					1905:1912	The supervised orthogonal projection to latent structures discriminant analysis	1834:1912	The supervised orthogonal projection to latent structures discriminant analysis	1834:1912	The supervised orthogonal projection to latent structures discriminant analysis identified phenolic acids as the phenolic class mostly affected by the different extraction technologies.
30599948	9	53	theme	radical	1770:1776	arg1	capacity					1789:1796	oxygen radical absorbance capacity	1763:1796	the highest oxygen radical absorbance capacity (536.27 μmol Trolox Equivalents/g)	1751:1831	Homogenizer-assisted extraction of M. oleifera leaves using 100% methanol allowed extracting the highest amounts of polyphenols (35.19 mg/g) and produced the highest oxygen radical absorbance capacity (536.27 μmol Trolox Equivalents/g).
30599948	9	53	theme	radical	1770:1776	arg1	Equivalents/g					1818:1830	536.27 μmol Trolox Equivalents/g	1799:1830	536.27 μmol Trolox Equivalents/g	1799:1830	Homogenizer-assisted extraction of M. oleifera leaves using 100% methanol allowed extracting the highest amounts of polyphenols (35.19 mg/g) and produced the highest oxygen radical absorbance capacity (536.27 μmol Trolox Equivalents/g).
30599948	8	54	theme	methanol	1455:1462	arg1	%					1467:1467	methanol 100%	1455:1467	methanol 100%	1455:1467	Overall, the extractions in methanol 100% were found to be the most effective for phenolic compounds recovering, when compared with those in methanol/water (50:50, v/v).
30599948	10	55	theme	latent	1874:1879	arg1	structures					1881:1890	latent structures	1874:1890	latent structures	1874:1890	The supervised orthogonal projection to latent structures discriminant analysis identified phenolic acids as the phenolic class mostly affected by the different extraction technologies.
30599948	8	56	from	extractions	1440:1450	arg1	%					1467:1467	methanol 100%	1455:1467	methanol 100%	1455:1467	Overall, the extractions in methanol 100% were found to be the most effective for phenolic compounds recovering, when compared with those in methanol/water (50:50, v/v).
30599948	4	57	theme	oxygen	929:934	arg1	ORAC					965:968	ORAC	965:968	ORAC	965:968	Besides, the in vitro antioxidant activities of Moringa leaves were also investigated as ferric reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC).
30599948	4	57	theme	oxygen	929:934	arg1	activities					829:838	the in vitro antioxidant activities	804:838	the in vitro antioxidant activities of Moringa leaves	804:856	Besides, the in vitro antioxidant activities of Moringa leaves were also investigated as ferric reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC).
30599948	4	57	theme	oxygen	929:934	arg1	capacity					955:962	oxygen radical absorbance capacity	929:962	oxygen radical absorbance capacity (ORAC)	929:969	Besides, the in vitro antioxidant activities of Moringa leaves were also investigated as ferric reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC).
30599948	3	58	theme	liquid	634:639	arg1	chromatography					641:654	ultra-high-pressure liquid chromatography	614:654	ultra-high-pressure liquid chromatography coupled to a quadrupole-time-of-flight mass spectrometry	614:711	With this aim, ultra-high-pressure liquid chromatography coupled to a quadrupole-time-of-flight mass spectrometry was used to profile phenolic compounds under the different extraction conditions.
30599948	4	59	theme	absorbance	944:953	arg1	ORAC					965:968	ORAC	965:968	ORAC	965:968	Besides, the in vitro antioxidant activities of Moringa leaves were also investigated as ferric reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC).
30599948	4	59	theme	absorbance	944:953	arg1	activities					829:838	the in vitro antioxidant activities	804:838	the in vitro antioxidant activities of Moringa leaves	804:856	Besides, the in vitro antioxidant activities of Moringa leaves were also investigated as ferric reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC).
30599948	4	59	theme	absorbance	944:953	arg1	capacity					955:962	oxygen radical absorbance capacity	929:962	oxygen radical absorbance capacity (ORAC)	929:969	Besides, the in vitro antioxidant activities of Moringa leaves were also investigated as ferric reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC).
30599948	1	60	theme	rapid	218:222	arg1	methods					157:163	different extraction methods	136:163	different extraction methods	136:163	The impact of different extraction methods, namely maceration, homogenizer-assisted extraction, rapid solid-liquid dynamic extraction, microwave-assisted extraction and ultrasound-assisted extraction, on polyphenols of Moringa oleifera leaves was studied.
30599948	1	60	theme	rapid	218:222	arg1	extraction					245:254	rapid solid-liquid dynamic extraction	218:254	rapid solid-liquid dynamic extraction	218:254	The impact of different extraction methods, namely maceration, homogenizer-assisted extraction, rapid solid-liquid dynamic extraction, microwave-assisted extraction and ultrasound-assisted extraction, on polyphenols of Moringa oleifera leaves was studied.
30599948	9	61	theme	536.27 μmol	1799:1809	arg1	capacity					1789:1796	oxygen radical absorbance capacity	1763:1796	the highest oxygen radical absorbance capacity (536.27 μmol Trolox Equivalents/g)	1751:1831	Homogenizer-assisted extraction of M. oleifera leaves using 100% methanol allowed extracting the highest amounts of polyphenols (35.19 mg/g) and produced the highest oxygen radical absorbance capacity (536.27 μmol Trolox Equivalents/g).
30599948	9	61	theme	536.27 μmol	1799:1809	arg1	Equivalents/g					1818:1830	536.27 μmol Trolox Equivalents/g	1799:1830	536.27 μmol Trolox Equivalents/g	1799:1830	Homogenizer-assisted extraction of M. oleifera leaves using 100% methanol allowed extracting the highest amounts of polyphenols (35.19 mg/g) and produced the highest oxygen radical absorbance capacity (536.27 μmol Trolox Equivalents/g).
30599948	1	62	theme	dynamic	237:243	arg1	methods					157:163	different extraction methods	136:163	different extraction methods	136:163	The impact of different extraction methods, namely maceration, homogenizer-assisted extraction, rapid solid-liquid dynamic extraction, microwave-assisted extraction and ultrasound-assisted extraction, on polyphenols of Moringa oleifera leaves was studied.
30599948	1	62	theme	dynamic	237:243	arg1	extraction					245:254	rapid solid-liquid dynamic extraction	218:254	rapid solid-liquid dynamic extraction	218:254	The impact of different extraction methods, namely maceration, homogenizer-assisted extraction, rapid solid-liquid dynamic extraction, microwave-assisted extraction and ultrasound-assisted extraction, on polyphenols of Moringa oleifera leaves was studied.
30599948	11	63	theme	phenolic	2109:2116	arg1	subclasses					2118:2127	specific phenolic subclasses	2100:2127	specific phenolic subclasses	2100:2127	These findings demonstrate that each extraction method promoted the recovery of specific phenolic subclasses with different efficiencies.
30599948	10	64	theme	orthogonal	1849:1858	arg1	analysis					1905:1912	The supervised orthogonal projection to latent structures discriminant analysis	1834:1912	The supervised orthogonal projection to latent structures discriminant analysis	1834:1912	The supervised orthogonal projection to latent structures discriminant analysis identified phenolic acids as the phenolic class mostly affected by the different extraction technologies.
30599948	9	65	theme	Homogenizer-assisted	1597:1616	arg1	extraction					1618:1627	Homogenizer-assisted extraction	1597:1627	Homogenizer-assisted extraction of M. oleifera leaves using 100% methanol	1597:1669	Homogenizer-assisted extraction of M. oleifera leaves using 100% methanol allowed extracting the highest amounts of polyphenols (35.19 mg/g) and produced the highest oxygen radical absorbance capacity (536.27 μmol Trolox Equivalents/g).
30599948	3	66	theme	mass	695:698	arg1	spectrometry					700:711	a quadrupole-time-of-flight mass spectrometry	667:711	a quadrupole-time-of-flight mass spectrometry	667:711	With this aim, ultra-high-pressure liquid chromatography coupled to a quadrupole-time-of-flight mass spectrometry was used to profile phenolic compounds under the different extraction conditions.
30599948	1	67	theme	methods	157:163	arg1	impact					126:131	The impact	122:131	The impact of different extraction methods, namely maceration, homogenizer-assisted extraction, rapid solid-liquid dynamic extraction, microwave-assisted extraction and ultrasound-assisted extraction, on polyphenols of Moringa oleifera	122:356	The impact of different extraction methods, namely maceration, homogenizer-assisted extraction, rapid solid-liquid dynamic extraction, microwave-assisted extraction and ultrasound-assisted extraction, on polyphenols of Moringa oleifera leaves was studied.
30599948	6	68	theme	quercetin	1104:1112	arg1	3-O-galactoside					1114:1128	quercetin 3-O-galactoside	1104:1128	quercetin 3-O-galactoside	1104:1128	In particular, glycosidic forms of quercetin (i.e., quercetin 3-O-galactoside, quercetin 3-O-glucoside, and quercetin 4'-O-glucoside) were the most represented compounds among flavonoids.
30599948	2	69	theme	profiling	543:551	arg1	approach					553:560	an untargeted metabolomics-based profiling approach	510:560	an untargeted metabolomics-based profiling approach followed by multivariate statistics	510:596	The phenolic composition of alcoholic (methanol 100%) and hydroalcoholic (methanol/water 50:50, v/v) extracts was compared by using an untargeted metabolomics-based profiling approach followed by multivariate statistics.
30599948	0	70	theme	Moringa	97:103	arg1	oleifera					105:112	Moringa oleifera	97:112	Moringa oleifera	97:112	Impact of conventional/non-conventional extraction methods on the untargeted phenolic profile of Moringa oleifera leaves.
30599948	9	71	theme	oleifera	1635:1642	arg1	extraction					1618:1627	Homogenizer-assisted extraction	1597:1627	Homogenizer-assisted extraction of M. oleifera leaves using 100% methanol	1597:1669	Homogenizer-assisted extraction of M. oleifera leaves using 100% methanol allowed extracting the highest amounts of polyphenols (35.19 mg/g) and produced the highest oxygen radical absorbance capacity (536.27 μmol Trolox Equivalents/g).
30599948	11	72	theme	different	2134:2142	arg1	efficiencies					2144:2155	different efficiencies	2134:2155	different efficiencies	2134:2155	These findings demonstrate that each extraction method promoted the recovery of specific phenolic subclasses with different efficiencies.
30599948	2	73	theme	untargeted	513:522	arg1	approach					553:560	an untargeted metabolomics-based profiling approach	510:560	an untargeted metabolomics-based profiling approach followed by multivariate statistics	510:596	The phenolic composition of alcoholic (methanol 100%) and hydroalcoholic (methanol/water 50:50, v/v) extracts was compared by using an untargeted metabolomics-based profiling approach followed by multivariate statistics.
30599948	7	74	theme	hydroxybenzaldheyde	1307:1325	arg1	acid					1268:1271	protocatechuic acid	1253:1271	protocatechuic acid	1253:1271	Furthermore, protocatechuic acid was found to be the most abundant hydroxybenzaldheyde derivative, while the isomeric forms of hydroxybenzoic acid characterized the phenolic acids class.
30599948	7	74	theme	hydroxybenzaldheyde	1307:1325	arg1	derivative					1327:1336	the most abundant hydroxybenzaldheyde derivative	1289:1336	the most abundant hydroxybenzaldheyde derivative	1289:1336	Furthermore, protocatechuic acid was found to be the most abundant hydroxybenzaldheyde derivative, while the isomeric forms of hydroxybenzoic acid characterized the phenolic acids class.
30599948	6	75	theme	quercetin	1087:1095	arg1	compounds					1212:1220	the most represented compounds	1191:1220	the most represented compounds among flavonoids	1191:1237	In particular, glycosidic forms of quercetin (i.e., quercetin 3-O-galactoside, quercetin 3-O-glucoside, and quercetin 4'-O-glucoside) were the most represented compounds among flavonoids.
30599948	6	75	theme	quercetin	1087:1095	arg1	forms					1078:1082	glycosidic forms	1067:1082	glycosidic forms of quercetin (i.e., quercetin 3-O-galactoside, quercetin 3-O-glucoside, and quercetin 4'-O-glucoside)	1067:1184	In particular, glycosidic forms of quercetin (i.e., quercetin 3-O-galactoside, quercetin 3-O-glucoside, and quercetin 4'-O-glucoside) were the most represented compounds among flavonoids.
30599948	2	76	theme	multivariate	574:585	arg1	statistics					587:596	multivariate statistics	574:596	multivariate statistics	574:596	The phenolic composition of alcoholic (methanol 100%) and hydroalcoholic (methanol/water 50:50, v/v) extracts was compared by using an untargeted metabolomics-based profiling approach followed by multivariate statistics.
30599948	2	77	dep	alcoholic	406:414	arg1	methanol/water					452:465	methanol/water	452:465	methanol/water	452:465	The phenolic composition of alcoholic (methanol 100%) and hydroalcoholic (methanol/water 50:50, v/v) extracts was compared by using an untargeted metabolomics-based profiling approach followed by multivariate statistics.
30599948	2	77	dep	alcoholic	406:414	arg1	%					429:429	methanol 100%	417:429	methanol 100%	417:429	The phenolic composition of alcoholic (methanol 100%) and hydroalcoholic (methanol/water 50:50, v/v) extracts was compared by using an untargeted metabolomics-based profiling approach followed by multivariate statistics.
30599948	9	78	theme	%	1660:1660	arg1	methanol					1662:1669	100% methanol	1657:1669	100% methanol	1657:1669	Homogenizer-assisted extraction of M. oleifera leaves using 100% methanol allowed extracting the highest amounts of polyphenols (35.19 mg/g) and produced the highest oxygen radical absorbance capacity (536.27 μmol Trolox Equivalents/g).
30599948	5	79	theme	phenolic	1032:1039	arg1	compounds					1041:1049	262 phenolic compounds	1028:1049	262 phenolic compounds	1028:1049	The metabolomic approach allowed to putatively annotate 262 phenolic compounds.
30599948	2	80	theme	extracts	479:486	arg1	composition					391:401	The phenolic composition	378:401	The phenolic composition of alcoholic (methanol 100%) and hydroalcoholic (methanol/water 50:50, v/v) extracts	378:486	The phenolic composition of alcoholic (methanol 100%) and hydroalcoholic (methanol/water 50:50, v/v) extracts was compared by using an untargeted metabolomics-based profiling approach followed by multivariate statistics.
30599948	11	81	theme	extraction	2057:2066	arg1	method					2068:2073	each extraction method	2052:2073	each extraction method	2052:2073	These findings demonstrate that each extraction method promoted the recovery of specific phenolic subclasses with different efficiencies.
30599948	0	82	theme	extraction	40:49	arg1	methods					51:57	conventional/non-conventional extraction methods	10:57	conventional/non-conventional extraction methods	10:57	Impact of conventional/non-conventional extraction methods on the untargeted phenolic profile of Moringa oleifera leaves.
30599948	4	83	dep	in	808:809	arg1	vitro					811:815	vitro	811:815	vitro	811:815	Besides, the in vitro antioxidant activities of Moringa leaves were also investigated as ferric reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC).
30599948	3	84	theme	phenolic	733:740	arg1	compounds					742:750	phenolic compounds	733:750	phenolic compounds	733:750	With this aim, ultra-high-pressure liquid chromatography coupled to a quadrupole-time-of-flight mass spectrometry was used to profile phenolic compounds under the different extraction conditions.
30599948	4	85	theme	Moringa	843:849	arg1	capacity					955:962	oxygen radical absorbance capacity	929:962	oxygen radical absorbance capacity (ORAC)	929:969	Besides, the in vitro antioxidant activities of Moringa leaves were also investigated as ferric reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC).
30599948	4	85	theme	Moringa	843:849	arg1	activities					829:838	the in vitro antioxidant activities	804:838	the in vitro antioxidant activities of Moringa leaves	804:856	Besides, the in vitro antioxidant activities of Moringa leaves were also investigated as ferric reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC).
30599948	4	85	theme	Moringa	843:849	arg1	power					912:916	ferric reducing antioxidant power	884:916	ferric reducing antioxidant power (FRAP)	884:923	Besides, the in vitro antioxidant activities of Moringa leaves were also investigated as ferric reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC).
30599948	11	86	theme	specific	2100:2107	arg1	subclasses					2118:2127	specific phenolic subclasses	2100:2127	specific phenolic subclasses	2100:2127	These findings demonstrate that each extraction method promoted the recovery of specific phenolic subclasses with different efficiencies.
30599948	1	87	theme	oleifera	349:356	arg1	polyphenols					326:336	polyphenols	326:336	polyphenols of Moringa oleifera	326:356	The impact of different extraction methods, namely maceration, homogenizer-assisted extraction, rapid solid-liquid dynamic extraction, microwave-assisted extraction and ultrasound-assisted extraction, on polyphenols of Moringa oleifera leaves was studied.
30599948	10	88	theme	extraction	1995:2004	arg1	technologies					2006:2017	the different extraction technologies	1981:2017	the different extraction technologies	1981:2017	The supervised orthogonal projection to latent structures discriminant analysis identified phenolic acids as the phenolic class mostly affected by the different extraction technologies.
30599948	0	89	theme	untargeted	66:75	arg1	profile					86:92	the untargeted phenolic profile	62:92	the untargeted phenolic profile of Moringa oleifera	62:112	Impact of conventional/non-conventional extraction methods on the untargeted phenolic profile of Moringa oleifera leaves.
30599948	9	90	theme	polyphenols	1713:1723	arg1	polyphenols					1713:1723	polyphenols	1713:1723	polyphenols (35.19 mg/g)	1713:1736	Homogenizer-assisted extraction of M. oleifera leaves using 100% methanol allowed extracting the highest amounts of polyphenols (35.19 mg/g) and produced the highest oxygen radical absorbance capacity (536.27 μmol Trolox Equivalents/g).
30599948	9	90	theme	polyphenols	1713:1723	arg1	amounts					1702:1708	the highest amounts	1690:1708	the highest amounts of polyphenols (35.19 mg/g)	1690:1736	Homogenizer-assisted extraction of M. oleifera leaves using 100% methanol allowed extracting the highest amounts of polyphenols (35.19 mg/g) and produced the highest oxygen radical absorbance capacity (536.27 μmol Trolox Equivalents/g).
30599948	9	90	theme	polyphenols	1713:1723	arg1	35.19 mg/g					1726:1735	35.19 mg/g	1726:1735	35.19 mg/g	1726:1735	Homogenizer-assisted extraction of M. oleifera leaves using 100% methanol allowed extracting the highest amounts of polyphenols (35.19 mg/g) and produced the highest oxygen radical absorbance capacity (536.27 μmol Trolox Equivalents/g).
30599948	7	91	theme	phenolic	1405:1412	arg1	class					1420:1424	the phenolic acids class	1401:1424	the phenolic acids class	1401:1424	Furthermore, protocatechuic acid was found to be the most abundant hydroxybenzaldheyde derivative, while the isomeric forms of hydroxybenzoic acid characterized the phenolic acids class.
30599948	3	92	theme	different	762:770	arg1	conditions					783:792	the different extraction conditions	758:792	the different extraction conditions	758:792	With this aim, ultra-high-pressure liquid chromatography coupled to a quadrupole-time-of-flight mass spectrometry was used to profile phenolic compounds under the different extraction conditions.
30599948	8	93	theme	phenolic	1509:1516	arg1	compounds					1518:1526	phenolic compounds	1509:1526	phenolic compounds recovering	1509:1537	Overall, the extractions in methanol 100% were found to be the most effective for phenolic compounds recovering, when compared with those in methanol/water (50:50, v/v).
30599948	10	94	theme	phenolic	1947:1954	arg1	class					1956:1960	the phenolic class	1943:1960	the phenolic class mostly affected by the different extraction technologies	1943:2017	The supervised orthogonal projection to latent structures discriminant analysis identified phenolic acids as the phenolic class mostly affected by the different extraction technologies.
30599948	10	94	theme	phenolic	1947:1954	arg1	acids					1934:1938	phenolic acids	1925:1938	phenolic acids	1925:1938	The supervised orthogonal projection to latent structures discriminant analysis identified phenolic acids as the phenolic class mostly affected by the different extraction technologies.
30599948	4	95	theme	ferric	884:889	arg1	FRAP					919:922	FRAP	919:922	FRAP	919:922	Besides, the in vitro antioxidant activities of Moringa leaves were also investigated as ferric reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC).
30599948	4	95	theme	ferric	884:889	arg1	activities					829:838	the in vitro antioxidant activities	804:838	the in vitro antioxidant activities of Moringa leaves	804:856	Besides, the in vitro antioxidant activities of Moringa leaves were also investigated as ferric reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC).
30599948	4	95	theme	ferric	884:889	arg1	power					912:916	ferric reducing antioxidant power	884:916	ferric reducing antioxidant power (FRAP)	884:923	Besides, the in vitro antioxidant activities of Moringa leaves were also investigated as ferric reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC).
30599948	6	96	theme	represented	1200:1210	arg1	compounds					1212:1220	the most represented compounds	1191:1220	the most represented compounds among flavonoids	1191:1237	In particular, glycosidic forms of quercetin (i.e., quercetin 3-O-galactoside, quercetin 3-O-glucoside, and quercetin 4'-O-glucoside) were the most represented compounds among flavonoids.
30599948	6	96	theme	represented	1200:1210	arg1	forms					1078:1082	glycosidic forms	1067:1082	glycosidic forms of quercetin (i.e., quercetin 3-O-galactoside, quercetin 3-O-glucoside, and quercetin 4'-O-glucoside)	1067:1184	In particular, glycosidic forms of quercetin (i.e., quercetin 3-O-galactoside, quercetin 3-O-glucoside, and quercetin 4'-O-glucoside) were the most represented compounds among flavonoids.
30599948	1	97	theme	microwave-assisted	257:274	arg1	extraction					276:285	microwave-assisted extraction	257:285	microwave-assisted extraction	257:285	The impact of different extraction methods, namely maceration, homogenizer-assisted extraction, rapid solid-liquid dynamic extraction, microwave-assisted extraction and ultrasound-assisted extraction, on polyphenols of Moringa oleifera leaves was studied.
30599948	1	97	theme	microwave-assisted	257:274	arg1	methods					157:163	different extraction methods	136:163	different extraction methods	136:163	The impact of different extraction methods, namely maceration, homogenizer-assisted extraction, rapid solid-liquid dynamic extraction, microwave-assisted extraction and ultrasound-assisted extraction, on polyphenols of Moringa oleifera leaves was studied.
30599948	7	98	theme	hydroxybenzoic	1367:1380	arg1	acid					1382:1385	hydroxybenzoic acid	1367:1385	hydroxybenzoic acid	1367:1385	Furthermore, protocatechuic acid was found to be the most abundant hydroxybenzaldheyde derivative, while the isomeric forms of hydroxybenzoic acid characterized the phenolic acids class.
30599948	4	99	theme	antioxidant	900:910	arg1	FRAP					919:922	FRAP	919:922	FRAP	919:922	Besides, the in vitro antioxidant activities of Moringa leaves were also investigated as ferric reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC).
30599948	4	99	theme	antioxidant	900:910	arg1	activities					829:838	the in vitro antioxidant activities	804:838	the in vitro antioxidant activities of Moringa leaves	804:856	Besides, the in vitro antioxidant activities of Moringa leaves were also investigated as ferric reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC).
30599948	4	99	theme	antioxidant	900:910	arg1	power					912:916	ferric reducing antioxidant power	884:916	ferric reducing antioxidant power (FRAP)	884:923	Besides, the in vitro antioxidant activities of Moringa leaves were also investigated as ferric reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC).
30599948	6	100	from	compounds	1212:1220	arg1	particular					1055:1064	particular	1055:1064	particular	1055:1064	In particular, glycosidic forms of quercetin (i.e., quercetin 3-O-galactoside, quercetin 3-O-glucoside, and quercetin 4'-O-glucoside) were the most represented compounds among flavonoids.
30599948	2	101	theme	methanol	417:424	arg1	%					429:429	methanol 100%	417:429	methanol 100%	417:429	The phenolic composition of alcoholic (methanol 100%) and hydroalcoholic (methanol/water 50:50, v/v) extracts was compared by using an untargeted metabolomics-based profiling approach followed by multivariate statistics.
30362934	5	0	theme	JCM	717:719	arg1	%					734:734	98.3 %	729:734	98.3 %	729:734	Phylogenetic analysis based on the full-length 16S rRNA sequences revealed that type strain 78T and strain 601 belong to the genus Nocardioides, sharing the highest similarity to Nocardioides solisilvae JCM 31492T (98.3 %), Nocardioides gilvus XZ17T (97.4 %) and Nocardioides daejeonensis JCM 16922T (97.4 %).
30362934	5	0	theme	JCM	717:719	arg1	31492T					721:726	Nocardioides solisilvae JCM 31492T	693:726	Nocardioides solisilvae JCM 31492T (98.3 %)	693:735	Phylogenetic analysis based on the full-length 16S rRNA sequences revealed that type strain 78T and strain 601 belong to the genus Nocardioides, sharing the highest similarity to Nocardioides solisilvae JCM 31492T (98.3 %), Nocardioides gilvus XZ17T (97.4 %) and Nocardioides daejeonensis JCM 16922T (97.4 %).
30362934	10	1	theme	main	1382:1385	arg1	sugars					1398:1403	The main whole-cell sugars	1378:1403	The main whole-cell sugars were rhamnose, xylose and galactose	1378:1439	The main whole-cell sugars were rhamnose, xylose and galactose and the major fatty acids (>10 %) were C17 : 1ω8c, iso-C16 : 0 and C18 : 1ω9c.
30362934	10	1	theme	main	1382:1385	arg1	C17 					1480:1483	C17 	1480:1483	C17 	1480:1483	The main whole-cell sugars were rhamnose, xylose and galactose and the major fatty acids (>10 %) were C17 : 1ω8c, iso-C16 : 0 and C18 : 1ω9c.
30362934	12	2	theme	phylogenetic	1666:1677	arg1	analyses					1679:1686	the phenotypic, genotypic and phylogenetic analyses	1636:1686	the phenotypic, genotypic and phylogenetic analyses	1636:1686	Based on evidence collected from the phenotypic, genotypic and phylogenetic analyses, we propose a novel species named Nocardioideshouyundeii sp.
30362934	14	3	theme	=CGMCC	1778:1783	arg1	106424T					1797:1803	=CGMCC 4.7461T=DSM 106424T	1778:1803	=CGMCC 4.7461T=DSM 106424T	1778:1803	The type strain is 78T (=CGMCC 4.7461T=DSM 106424T).
30362934	14	3	theme	=CGMCC	1778:1783	arg1	78T					1773:1775	78T	1773:1775	78T (=CGMCC 4.7461T=DSM 106424T)	1773:1804	The type strain is 78T (=CGMCC 4.7461T=DSM 106424T).
30362934	4	4	from	cream	468:472	arg1	circular					485:492	circular	485:492	circular	485:492	When cultured on brain-heart infusion agar supplemented with 5 % sheep blood, colonies were cream in colour, circular, smooth and convex.
30362934	4	4	from	cream	468:472	arg1	colour					477:482	colour	477:482	colour	477:482	When cultured on brain-heart infusion agar supplemented with 5 % sheep blood, colonies were cream in colour, circular, smooth and convex.
30362934	4	4	from	cream	468:472	arg1	convex					506:511	convex	506:511	convex	506:511	When cultured on brain-heart infusion agar supplemented with 5 % sheep blood, colonies were cream in colour, circular, smooth and convex.
30362934	4	4	from	cream	468:472	arg1	smooth					495:500	smooth	495:500	smooth	495:500	When cultured on brain-heart infusion agar supplemented with 5 % sheep blood, colonies were cream in colour, circular, smooth and convex.
30362934	6	5	theme	%	988:988	arg1	 threshold					989:998	the 95-96 % threshold	978:998	the 95-96 % threshold	978:998	The average nucleotide identity values between the two novel strains and the three closely related type strains of the genus Nocardioides were lower than the 95-96 % threshold.
30362934	2	6	from	Reserve	273:279	arg1	faeces					223:228	the faeces	219:228	the faeces of Tibetan antelopes at the Hoh Xil Nature Reserve, Qinghai-Tibet Plateau, China	219:309	In the present study, we describe two novel Gram-stain-positive, irregular rod-shaped bacterial strains, 78T and 601, that had been isolated from the faeces of Tibetan antelopes at the Hoh Xil Nature Reserve, Qinghai-Tibet Plateau, China.
30362934	2	7	attach	isolated	205:212	arg2	601					186:188	601	186:188	601	186:188	In the present study, we describe two novel Gram-stain-positive, irregular rod-shaped bacterial strains, 78T and 601, that had been isolated from the faeces of Tibetan antelopes at the Hoh Xil Nature Reserve, Qinghai-Tibet Plateau, China.
30362934	2	7	attach	isolated	205:212	arg1	faeces					223:228	the faeces	219:228	the faeces of Tibetan antelopes at the Hoh Xil Nature Reserve, Qinghai-Tibet Plateau, China	219:309	In the present study, we describe two novel Gram-stain-positive, irregular rod-shaped bacterial strains, 78T and 601, that had been isolated from the faeces of Tibetan antelopes at the Hoh Xil Nature Reserve, Qinghai-Tibet Plateau, China.
30362934	2	7	attach	isolated	205:212	arg2	78T					178:180	78T	178:180	78T	178:180	In the present study, we describe two novel Gram-stain-positive, irregular rod-shaped bacterial strains, 78T and 601, that had been isolated from the faeces of Tibetan antelopes at the Hoh Xil Nature Reserve, Qinghai-Tibet Plateau, China.
30362934	2	7	attach	isolated	205:212	arg2	strains					169:175	two novel Gram-stain-positive, irregular rod-shaped bacterial strains	107:175	two novel Gram-stain-positive, irregular rod-shaped bacterial strains	107:175	In the present study, we describe two novel Gram-stain-positive, irregular rod-shaped bacterial strains, 78T and 601, that had been isolated from the faeces of Tibetan antelopes at the Hoh Xil Nature Reserve, Qinghai-Tibet Plateau, China.
30362934	7	8	theme	DNA	1005:1007	arg1	G+C content					1009:1019	The DNA G+C content	1001:1019	The DNA G+C content of strains 78T and 601	1001:1042	The DNA G+C content of strains 78T and 601 were 71.2 and 71.3 mol% respectively.
30362934	7	8	theme	DNA	1005:1007	arg1	71.2					1049:1052	71.2	1049:1052	71.2	1049:1052	The DNA G+C content of strains 78T and 601 were 71.2 and 71.3 mol% respectively.
30362934	5	9	theme	full-length	549:559	arg1	sequences					570:578	the full-length 16S rRNA sequences	545:578	the full-length 16S rRNA sequences	545:578	Phylogenetic analysis based on the full-length 16S rRNA sequences revealed that type strain 78T and strain 601 belong to the genus Nocardioides, sharing the highest similarity to Nocardioides solisilvae JCM 31492T (98.3 %), Nocardioides gilvus XZ17T (97.4 %) and Nocardioides daejeonensis JCM 16922T (97.4 %).
30362934	1	10	theme	Tibetan	48:54	arg1	faeces					65:70	Tibetan antelope faeces	48:70	Tibetan antelope faeces	48:70	nov., isolated from Tibetan antelope faeces.
30362934	5	11	theme	highest	671:677	arg1	similarity					679:688	the highest similarity	667:688	the highest similarity to Nocardioides solisilvae JCM 31492T (98.3 %), Nocardioides gilvus XZ17T (97.4 %) and Nocardioides daejeonensis JCM 16922T (97.4 %)	667:821	Phylogenetic analysis based on the full-length 16S rRNA sequences revealed that type strain 78T and strain 601 belong to the genus Nocardioides, sharing the highest similarity to Nocardioides solisilvae JCM 31492T (98.3 %), Nocardioides gilvus XZ17T (97.4 %) and Nocardioides daejeonensis JCM 16922T (97.4 %).
30362934	4	12	theme	sheep	441:445	arg1	blood					447:451	5 % sheep blood	437:451	5 % sheep blood	437:451	When cultured on brain-heart infusion agar supplemented with 5 % sheep blood, colonies were cream in colour, circular, smooth and convex.
30362934	5	13	dep	Nocardioides	738:749	arg1	gilvus					751:756	gilvus	751:756	gilvus	751:756	Phylogenetic analysis based on the full-length 16S rRNA sequences revealed that type strain 78T and strain 601 belong to the genus Nocardioides, sharing the highest similarity to Nocardioides solisilvae JCM 31492T (98.3 %), Nocardioides gilvus XZ17T (97.4 %) and Nocardioides daejeonensis JCM 16922T (97.4 %).
30362934	5	14	theme	16S	561:563	arg1	sequences					570:578	the full-length 16S rRNA sequences	545:578	the full-length 16S rRNA sequences	545:578	Phylogenetic analysis based on the full-length 16S rRNA sequences revealed that type strain 78T and strain 601 belong to the genus Nocardioides, sharing the highest similarity to Nocardioides solisilvae JCM 31492T (98.3 %), Nocardioides gilvus XZ17T (97.4 %) and Nocardioides daejeonensis JCM 16922T (97.4 %).
30362934	1	15	theme	antelope	56:63	arg1	faeces					65:70	Tibetan antelope faeces	48:70	Tibetan antelope faeces	48:70	nov., isolated from Tibetan antelope faeces.
30362934	9	16	theme	unidentified	1358:1369	arg1	lipid					1371:1375	an unidentified lipid	1355:1375	an unidentified lipid	1355:1375	Its polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, an unidentified phospholipid and an unidentified lipid.
30362934	4	17	theme	%	439:439	arg1	blood					447:451	5 % sheep blood	437:451	5 % sheep blood	437:451	When cultured on brain-heart infusion agar supplemented with 5 % sheep blood, colonies were cream in colour, circular, smooth and convex.
30362934	5	18	theme	rRNA	565:568	arg1	sequences					570:578	the full-length 16S rRNA sequences	545:578	the full-length 16S rRNA sequences	545:578	Phylogenetic analysis based on the full-length 16S rRNA sequences revealed that type strain 78T and strain 601 belong to the genus Nocardioides, sharing the highest similarity to Nocardioides solisilvae JCM 31492T (98.3 %), Nocardioides gilvus XZ17T (97.4 %) and Nocardioides daejeonensis JCM 16922T (97.4 %).
30362934	2	19	theme	antelopes	241:249	arg1	faeces					223:228	the faeces	219:228	the faeces of Tibetan antelopes at the Hoh Xil Nature Reserve, Qinghai-Tibet Plateau, China	219:309	In the present study, we describe two novel Gram-stain-positive, irregular rod-shaped bacterial strains, 78T and 601, that had been isolated from the faeces of Tibetan antelopes at the Hoh Xil Nature Reserve, Qinghai-Tibet Plateau, China.
30362934	6	20	theme	95-96 	982:987	arg1	%					988:988	%	988:988	%	988:988	The average nucleotide identity values between the two novel strains and the three closely related type strains of the genus Nocardioides were lower than the 95-96 % threshold.
30362934	2	21	theme	Gram-stain-positive	117:135	arg1	strains					169:175	two novel Gram-stain-positive, irregular rod-shaped bacterial strains	107:175	two novel Gram-stain-positive, irregular rod-shaped bacterial strains	107:175	In the present study, we describe two novel Gram-stain-positive, irregular rod-shaped bacterial strains, 78T and 601, that had been isolated from the faeces of Tibetan antelopes at the Hoh Xil Nature Reserve, Qinghai-Tibet Plateau, China.
30362934	2	21	theme	Gram-stain-positive	117:135	arg1	601					186:188	601	186:188	601	186:188	In the present study, we describe two novel Gram-stain-positive, irregular rod-shaped bacterial strains, 78T and 601, that had been isolated from the faeces of Tibetan antelopes at the Hoh Xil Nature Reserve, Qinghai-Tibet Plateau, China.
30362934	2	21	theme	Gram-stain-positive	117:135	arg1	78T					178:180	78T	178:180	78T	178:180	In the present study, we describe two novel Gram-stain-positive, irregular rod-shaped bacterial strains, 78T and 601, that had been isolated from the faeces of Tibetan antelopes at the Hoh Xil Nature Reserve, Qinghai-Tibet Plateau, China.
30362934	5	22	theme	Phylogenetic	514:525	arg1	analysis					527:534	Phylogenetic analysis	514:534	Phylogenetic analysis based on the full-length 16S rRNA sequences	514:578	Phylogenetic analysis based on the full-length 16S rRNA sequences revealed that type strain 78T and strain 601 belong to the genus Nocardioides, sharing the highest similarity to Nocardioides solisilvae JCM 31492T (98.3 %), Nocardioides gilvus XZ17T (97.4 %) and Nocardioides daejeonensis JCM 16922T (97.4 %).
30362934	5	23	theme	Nocardioides	777:788	arg1	16922T					807:812	Nocardioides daejeonensis JCM 16922T	777:812	Nocardioides daejeonensis JCM 16922T (97.4 %)	777:821	Phylogenetic analysis based on the full-length 16S rRNA sequences revealed that type strain 78T and strain 601 belong to the genus Nocardioides, sharing the highest similarity to Nocardioides solisilvae JCM 31492T (98.3 %), Nocardioides gilvus XZ17T (97.4 %) and Nocardioides daejeonensis JCM 16922T (97.4 %).
30362934	5	23	theme	Nocardioides	777:788	arg1	%					820:820	97.4 %	815:820	97.4 %	815:820	Phylogenetic analysis based on the full-length 16S rRNA sequences revealed that type strain 78T and strain 601 belong to the genus Nocardioides, sharing the highest similarity to Nocardioides solisilvae JCM 31492T (98.3 %), Nocardioides gilvus XZ17T (97.4 %) and Nocardioides daejeonensis JCM 16922T (97.4 %).
30362934	0	24	theme	houyundeii	13:22	arg1	sp					24:25	houyundeii sp	13:25	houyundeii sp	13:25	Nocardioides houyundeii sp.
30362934	2	25	theme	novel	111:115	arg1	strains					169:175	two novel Gram-stain-positive, irregular rod-shaped bacterial strains	107:175	two novel Gram-stain-positive, irregular rod-shaped bacterial strains	107:175	In the present study, we describe two novel Gram-stain-positive, irregular rod-shaped bacterial strains, 78T and 601, that had been isolated from the faeces of Tibetan antelopes at the Hoh Xil Nature Reserve, Qinghai-Tibet Plateau, China.
30362934	2	25	theme	novel	111:115	arg1	601					186:188	601	186:188	601	186:188	In the present study, we describe two novel Gram-stain-positive, irregular rod-shaped bacterial strains, 78T and 601, that had been isolated from the faeces of Tibetan antelopes at the Hoh Xil Nature Reserve, Qinghai-Tibet Plateau, China.
30362934	2	25	theme	novel	111:115	arg1	78T					178:180	78T	178:180	78T	178:180	In the present study, we describe two novel Gram-stain-positive, irregular rod-shaped bacterial strains, 78T and 601, that had been isolated from the faeces of Tibetan antelopes at the Hoh Xil Nature Reserve, Qinghai-Tibet Plateau, China.
30362934	12	26	theme	novel	1702:1706	arg1	species					1708:1714	a novel species	1700:1714	a novel species named Nocardioideshouyundeii sp	1700:1746	Based on evidence collected from the phenotypic, genotypic and phylogenetic analyses, we propose a novel species named Nocardioideshouyundeii sp.
30362934	5	27	theme	Nocardioides	738:749	arg1	%					770:770	97.4 %	765:770	97.4 %	765:770	Phylogenetic analysis based on the full-length 16S rRNA sequences revealed that type strain 78T and strain 601 belong to the genus Nocardioides, sharing the highest similarity to Nocardioides solisilvae JCM 31492T (98.3 %), Nocardioides gilvus XZ17T (97.4 %) and Nocardioides daejeonensis JCM 16922T (97.4 %).
30362934	5	27	theme	Nocardioides	738:749	arg1	XZ17T					758:762	Nocardioides gilvus XZ17T	738:762	Nocardioides gilvus XZ17T (97.4 %)	738:771	Phylogenetic analysis based on the full-length 16S rRNA sequences revealed that type strain 78T and strain 601 belong to the genus Nocardioides, sharing the highest similarity to Nocardioides solisilvae JCM 31492T (98.3 %), Nocardioides gilvus XZ17T (97.4 %) and Nocardioides daejeonensis JCM 16922T (97.4 %).
30362934	2	28	dep	Gram-stain-positive	117:135	arg1	irregular					138:146	irregular	138:146	irregular	138:146	In the present study, we describe two novel Gram-stain-positive, irregular rod-shaped bacterial strains, 78T and 601, that had been isolated from the faeces of Tibetan antelopes at the Hoh Xil Nature Reserve, Qinghai-Tibet Plateau, China.
30362934	5	29	theme	type	594:597	arg1	strain					599:604	type strain 78T	594:608	type strain 78T	594:608	Phylogenetic analysis based on the full-length 16S rRNA sequences revealed that type strain 78T and strain 601 belong to the genus Nocardioides, sharing the highest similarity to Nocardioides solisilvae JCM 31492T (98.3 %), Nocardioides gilvus XZ17T (97.4 %) and Nocardioides daejeonensis JCM 16922T (97.4 %).
30362934	2	30	theme	Nature	266:271	arg1	Reserve					273:279	the Hoh Xil Nature Reserve	254:279	the Hoh Xil Nature Reserve	254:279	In the present study, we describe two novel Gram-stain-positive, irregular rod-shaped bacterial strains, 78T and 601, that had been isolated from the faeces of Tibetan antelopes at the Hoh Xil Nature Reserve, Qinghai-Tibet Plateau, China.
30362934	8	31	theme	ll-2,6-diaminopimelic	1136:1156	arg1	acid					1190:1193	the diagnostic diamino acid	1167:1193	the diagnostic diamino acid in its cell-wall peptidoglycan	1167:1224	MK-8 (H4) was the predominant respiratory quinone and ll-2,6-diaminopimelic acid was the diagnostic diamino acid in its cell-wall peptidoglycan.
30362934	8	31	theme	ll-2,6-diaminopimelic	1136:1156	arg1	acid					1158:1161	ll-2,6-diaminopimelic acid	1136:1161	ll-2,6-diaminopimelic acid	1136:1161	MK-8 (H4) was the predominant respiratory quinone and ll-2,6-diaminopimelic acid was the diagnostic diamino acid in its cell-wall peptidoglycan.
30362934	5	32	theme	JCM	803:805	arg1	16922T					807:812	Nocardioides daejeonensis JCM 16922T	777:812	Nocardioides daejeonensis JCM 16922T (97.4 %)	777:821	Phylogenetic analysis based on the full-length 16S rRNA sequences revealed that type strain 78T and strain 601 belong to the genus Nocardioides, sharing the highest similarity to Nocardioides solisilvae JCM 31492T (98.3 %), Nocardioides gilvus XZ17T (97.4 %) and Nocardioides daejeonensis JCM 16922T (97.4 %).
30362934	5	32	theme	JCM	803:805	arg1	%					820:820	97.4 %	815:820	97.4 %	815:820	Phylogenetic analysis based on the full-length 16S rRNA sequences revealed that type strain 78T and strain 601 belong to the genus Nocardioides, sharing the highest similarity to Nocardioides solisilvae JCM 31492T (98.3 %), Nocardioides gilvus XZ17T (97.4 %) and Nocardioides daejeonensis JCM 16922T (97.4 %).
30362934	6	33	theme	novel	879:883	arg1	strains					885:891	the two novel strains	871:891	the two novel strains	871:891	The average nucleotide identity values between the two novel strains and the three closely related type strains of the genus Nocardioides were lower than the 95-96 % threshold.
30362934	5	34	theme	genus	639:643	arg1	Nocardioides					645:656	the genus Nocardioides	635:656	the genus Nocardioides	635:656	Phylogenetic analysis based on the full-length 16S rRNA sequences revealed that type strain 78T and strain 601 belong to the genus Nocardioides, sharing the highest similarity to Nocardioides solisilvae JCM 31492T (98.3 %), Nocardioides gilvus XZ17T (97.4 %) and Nocardioides daejeonensis JCM 16922T (97.4 %).
30362934	12	35	theme	phenotypic	1640:1649	arg1	analyses					1679:1686	the phenotypic, genotypic and phylogenetic analyses	1636:1686	the phenotypic, genotypic and phylogenetic analyses	1636:1686	Based on evidence collected from the phenotypic, genotypic and phylogenetic analyses, we propose a novel species named Nocardioideshouyundeii sp.
30362934	2	36	theme	Xil	262:264	arg1	Reserve					273:279	the Hoh Xil Nature Reserve	254:279	the Hoh Xil Nature Reserve	254:279	In the present study, we describe two novel Gram-stain-positive, irregular rod-shaped bacterial strains, 78T and 601, that had been isolated from the faeces of Tibetan antelopes at the Hoh Xil Nature Reserve, Qinghai-Tibet Plateau, China.
30362934	10	37	theme	whole-cell	1387:1396	arg1	sugars					1398:1403	The main whole-cell sugars	1378:1403	The main whole-cell sugars were rhamnose, xylose and galactose	1378:1439	The main whole-cell sugars were rhamnose, xylose and galactose and the major fatty acids (>10 %) were C17 : 1ω8c, iso-C16 : 0 and C18 : 1ω9c.
30362934	10	37	theme	whole-cell	1387:1396	arg1	C17 					1480:1483	C17 	1480:1483	C17 	1480:1483	The main whole-cell sugars were rhamnose, xylose and galactose and the major fatty acids (>10 %) were C17 : 1ω8c, iso-C16 : 0 and C18 : 1ω9c.
30362934	7	38	dep	strains	1024:1030	arg1	601					1040:1042	601	1040:1042	601	1040:1042	The DNA G+C content of strains 78T and 601 were 71.2 and 71.3 mol% respectively.
30362934	7	38	dep	strains	1024:1030	arg1	78T					1032:1034	78T	1032:1034	78T	1032:1034	The DNA G+C content of strains 78T and 601 were 71.2 and 71.3 mol% respectively.
30362934	2	39	theme	Hoh	258:260	arg1	Reserve					273:279	the Hoh Xil Nature Reserve	254:279	the Hoh Xil Nature Reserve	254:279	In the present study, we describe two novel Gram-stain-positive, irregular rod-shaped bacterial strains, 78T and 601, that had been isolated from the faeces of Tibetan antelopes at the Hoh Xil Nature Reserve, Qinghai-Tibet Plateau, China.
30362934	6	40	theme	Nocardioides	949:960	arg1	strains					928:934	the three closely related type strains	897:934	the three closely related type strains of the genus Nocardioides	897:960	The average nucleotide identity values between the two novel strains and the three closely related type strains of the genus Nocardioides were lower than the 95-96 % threshold.
30362934	6	40	theme	Nocardioides	949:960	arg1	strains					885:891	the two novel strains	871:891	the two novel strains	871:891	The average nucleotide identity values between the two novel strains and the three closely related type strains of the genus Nocardioides were lower than the 95-96 % threshold.
30362934	8	41	theme	diagnostic	1171:1180	arg1	acid					1190:1193	the diagnostic diamino acid	1167:1193	the diagnostic diamino acid in its cell-wall peptidoglycan	1167:1224	MK-8 (H4) was the predominant respiratory quinone and ll-2,6-diaminopimelic acid was the diagnostic diamino acid in its cell-wall peptidoglycan.
30362934	8	41	theme	diagnostic	1171:1180	arg1	acid					1158:1161	ll-2,6-diaminopimelic acid	1136:1161	ll-2,6-diaminopimelic acid	1136:1161	MK-8 (H4) was the predominant respiratory quinone and ll-2,6-diaminopimelic acid was the diagnostic diamino acid in its cell-wall peptidoglycan.
30362934	14	42	theme	type	1758:1761	arg1	strain					1763:1768	The type strain	1754:1768	The type strain	1754:1768	The type strain is 78T (=CGMCC 4.7461T=DSM 106424T).
30362934	14	42	theme	type	1758:1761	arg1	78T					1773:1775	78T	1773:1775	78T (=CGMCC 4.7461T=DSM 106424T)	1773:1804	The type strain is 78T (=CGMCC 4.7461T=DSM 106424T).
30362934	2	43	from	faeces	223:228	arg1	Reserve					273:279	the Hoh Xil Nature Reserve	254:279	the Hoh Xil Nature Reserve	254:279	In the present study, we describe two novel Gram-stain-positive, irregular rod-shaped bacterial strains, 78T and 601, that had been isolated from the faeces of Tibetan antelopes at the Hoh Xil Nature Reserve, Qinghai-Tibet Plateau, China.
30362934	6	44	theme	genus	943:947	arg1	Nocardioides					949:960	the genus Nocardioides	939:960	the genus Nocardioides	939:960	The average nucleotide identity values between the two novel strains and the three closely related type strains of the genus Nocardioides were lower than the 95-96 % threshold.
30362934	8	45	theme	respiratory	1112:1122	arg1	quinone					1124:1130	the predominant respiratory quinone	1096:1130	the predominant respiratory quinone	1096:1130	MK-8 (H4) was the predominant respiratory quinone and ll-2,6-diaminopimelic acid was the diagnostic diamino acid in its cell-wall peptidoglycan.
30362934	8	45	theme	respiratory	1112:1122	arg1	MK-8					1082:1085	MK-8	1082:1085	MK-8 (H4)	1082:1090	MK-8 (H4) was the predominant respiratory quinone and ll-2,6-diaminopimelic acid was the diagnostic diamino acid in its cell-wall peptidoglycan.
30362934	11	46	theme	genus	1583:1587	arg1	Nocardioides					1589:1600	genus Nocardioides	1583:1600	genus Nocardioides	1583:1600	These data supported the affiliation of strains 78T and 601 to genus Nocardioides.
30362934	10	47	dep	C17 	1480:1483	arg1	 1ω8c					1485:1489	 1ω8c	1485:1489	 1ω8c	1485:1489	The main whole-cell sugars were rhamnose, xylose and galactose and the major fatty acids (>10 %) were C17 : 1ω8c, iso-C16 : 0 and C18 : 1ω9c.
30362934	10	47	dep	C17 	1480:1483	arg1	C18 					1508:1511	C18 	1508:1511	C18 	1508:1511	The main whole-cell sugars were rhamnose, xylose and galactose and the major fatty acids (>10 %) were C17 : 1ω8c, iso-C16 : 0 and C18 : 1ω9c.
30362934	10	47	dep	C17 	1480:1483	arg1	 1ω9c					1513:1517	 1ω9c	1513:1517	C17 : 1ω8c, iso-C16 : 0 and C18 : 1ω9c	1480:1517	The main whole-cell sugars were rhamnose, xylose and galactose and the major fatty acids (>10 %) were C17 : 1ω8c, iso-C16 : 0 and C18 : 1ω9c.
30362934	10	47	dep	C17 	1480:1483	arg1	 0					1501:1502	 0	1501:1502	 0	1501:1502	The main whole-cell sugars were rhamnose, xylose and galactose and the major fatty acids (>10 %) were C17 : 1ω8c, iso-C16 : 0 and C18 : 1ω9c.
30362934	4	48	theme	5 	437:438	arg1	%					439:439	%	439:439	%	439:439	When cultured on brain-heart infusion agar supplemented with 5 % sheep blood, colonies were cream in colour, circular, smooth and convex.
30362934	9	49	theme	polar	1231:1235	arg1	lipids					1237:1242	Its polar lipids	1227:1242	Its polar lipids	1227:1242	Its polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, an unidentified phospholipid and an unidentified lipid.
30362934	9	50	contain	contained	1244:1252	arg2	phosphatidylinositol					1300:1319	phosphatidylinositol	1300:1319	phosphatidylinositol	1300:1319	Its polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, an unidentified phospholipid and an unidentified lipid.
30362934	9	50	contain	contained	1244:1252	arg2	diphosphatidylglycerol					1254:1275	diphosphatidylglycerol	1254:1275	diphosphatidylglycerol	1254:1275	Its polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, an unidentified phospholipid and an unidentified lipid.
30362934	9	50	contain	contained	1244:1252	arg1	lipids					1237:1242	Its polar lipids	1227:1242	Its polar lipids	1227:1242	Its polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, an unidentified phospholipid and an unidentified lipid.
30362934	9	50	contain	contained	1244:1252	arg2	lipid					1371:1375	an unidentified lipid	1355:1375	an unidentified lipid	1355:1375	Its polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, an unidentified phospholipid and an unidentified lipid.
30362934	9	50	contain	contained	1244:1252	arg2	phosphatidylglycerol					1278:1297	phosphatidylglycerol	1278:1297	phosphatidylglycerol	1278:1297	Its polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, an unidentified phospholipid and an unidentified lipid.
30362934	9	50	contain	contained	1244:1252	arg2	phospholipid					1338:1349	an unidentified phospholipid	1322:1349	an unidentified phospholipid	1322:1349	Its polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, an unidentified phospholipid and an unidentified lipid.
30362934	7	51	theme	strains	1024:1030	arg1	G+C content					1009:1019	The DNA G+C content	1001:1019	The DNA G+C content of strains 78T and 601	1001:1042	The DNA G+C content of strains 78T and 601 were 71.2 and 71.3 mol% respectively.
30362934	7	51	theme	strains	1024:1030	arg1	71.2					1049:1052	71.2	1049:1052	71.2	1049:1052	The DNA G+C content of strains 78T and 601 were 71.2 and 71.3 mol% respectively.
30362934	2	52	theme	present	80:86	arg1	study					88:92	the present study	76:92	the present study	76:92	In the present study, we describe two novel Gram-stain-positive, irregular rod-shaped bacterial strains, 78T and 601, that had been isolated from the faeces of Tibetan antelopes at the Hoh Xil Nature Reserve, Qinghai-Tibet Plateau, China.
30362934	10	53	theme	fatty	1455:1459	arg1	acids					1461:1465	the major fatty acids	1445:1465	the major fatty acids (>10 %)	1445:1473	The main whole-cell sugars were rhamnose, xylose and galactose and the major fatty acids (>10 %) were C17 : 1ω8c, iso-C16 : 0 and C18 : 1ω9c.
30362934	10	53	theme	fatty	1455:1459	arg1	%					1472:1472	>10 %	1468:1472	>10 %	1468:1472	The main whole-cell sugars were rhamnose, xylose and galactose and the major fatty acids (>10 %) were C17 : 1ω8c, iso-C16 : 0 and C18 : 1ω9c.
30362934	10	53	theme	fatty	1455:1459	arg1	C17 					1480:1483	C17 	1480:1483	C17 	1480:1483	The main whole-cell sugars were rhamnose, xylose and galactose and the major fatty acids (>10 %) were C17 : 1ω8c, iso-C16 : 0 and C18 : 1ω9c.
30362934	2	54	theme	rod-shaped	148:157	arg1	strains					169:175	two novel Gram-stain-positive, irregular rod-shaped bacterial strains	107:175	two novel Gram-stain-positive, irregular rod-shaped bacterial strains	107:175	In the present study, we describe two novel Gram-stain-positive, irregular rod-shaped bacterial strains, 78T and 601, that had been isolated from the faeces of Tibetan antelopes at the Hoh Xil Nature Reserve, Qinghai-Tibet Plateau, China.
30362934	2	54	theme	rod-shaped	148:157	arg1	601					186:188	601	186:188	601	186:188	In the present study, we describe two novel Gram-stain-positive, irregular rod-shaped bacterial strains, 78T and 601, that had been isolated from the faeces of Tibetan antelopes at the Hoh Xil Nature Reserve, Qinghai-Tibet Plateau, China.
30362934	2	54	theme	rod-shaped	148:157	arg1	78T					178:180	78T	178:180	78T	178:180	In the present study, we describe two novel Gram-stain-positive, irregular rod-shaped bacterial strains, 78T and 601, that had been isolated from the faeces of Tibetan antelopes at the Hoh Xil Nature Reserve, Qinghai-Tibet Plateau, China.
30362934	8	55	from	quinone	1124:1130	arg1	peptidoglycan					1212:1224	its cell-wall peptidoglycan	1198:1224	its cell-wall peptidoglycan	1198:1224	MK-8 (H4) was the predominant respiratory quinone and ll-2,6-diaminopimelic acid was the diagnostic diamino acid in its cell-wall peptidoglycan.
30362934	8	56	theme	diamino	1182:1188	arg1	acid					1190:1193	the diagnostic diamino acid	1167:1193	the diagnostic diamino acid in its cell-wall peptidoglycan	1167:1224	MK-8 (H4) was the predominant respiratory quinone and ll-2,6-diaminopimelic acid was the diagnostic diamino acid in its cell-wall peptidoglycan.
30362934	8	56	theme	diamino	1182:1188	arg1	acid					1158:1161	ll-2,6-diaminopimelic acid	1136:1161	ll-2,6-diaminopimelic acid	1136:1161	MK-8 (H4) was the predominant respiratory quinone and ll-2,6-diaminopimelic acid was the diagnostic diamino acid in its cell-wall peptidoglycan.
30362934	4	57	from	colour	477:482	arg1	cream					468:472	cream	468:472	cream	468:472	When cultured on brain-heart infusion agar supplemented with 5 % sheep blood, colonies were cream in colour, circular, smooth and convex.
30362934	6	58	theme	identity	847:854	arg1	values					856:861	The average nucleotide identity values	824:861	The average nucleotide identity values between the two novel strains and the three closely related type strains of the genus Nocardioides	824:960	The average nucleotide identity values between the two novel strains and the three closely related type strains of the genus Nocardioides were lower than the 95-96 % threshold.
30362934	6	58	theme	identity	847:854	arg1	lower					967:971	lower	967:971	lower	967:971	The average nucleotide identity values between the two novel strains and the three closely related type strains of the genus Nocardioides were lower than the 95-96 % threshold.
30362934	10	59	theme	major	1449:1453	arg1	acids					1461:1465	the major fatty acids	1445:1465	the major fatty acids (>10 %)	1445:1473	The main whole-cell sugars were rhamnose, xylose and galactose and the major fatty acids (>10 %) were C17 : 1ω8c, iso-C16 : 0 and C18 : 1ω9c.
30362934	10	59	theme	major	1449:1453	arg1	%					1472:1472	>10 %	1468:1472	>10 %	1468:1472	The main whole-cell sugars were rhamnose, xylose and galactose and the major fatty acids (>10 %) were C17 : 1ω8c, iso-C16 : 0 and C18 : 1ω9c.
30362934	10	59	theme	major	1449:1453	arg1	C17 					1480:1483	C17 	1480:1483	C17 	1480:1483	The main whole-cell sugars were rhamnose, xylose and galactose and the major fatty acids (>10 %) were C17 : 1ω8c, iso-C16 : 0 and C18 : 1ω9c.
30362934	5	60	dep	Nocardioides	777:788	arg1	daejeonensis					790:801	daejeonensis	790:801	daejeonensis	790:801	Phylogenetic analysis based on the full-length 16S rRNA sequences revealed that type strain 78T and strain 601 belong to the genus Nocardioides, sharing the highest similarity to Nocardioides solisilvae JCM 31492T (98.3 %), Nocardioides gilvus XZ17T (97.4 %) and Nocardioides daejeonensis JCM 16922T (97.4 %).
30362934	2	61	theme	bacterial	159:167	arg1	strains					169:175	two novel Gram-stain-positive, irregular rod-shaped bacterial strains	107:175	two novel Gram-stain-positive, irregular rod-shaped bacterial strains	107:175	In the present study, we describe two novel Gram-stain-positive, irregular rod-shaped bacterial strains, 78T and 601, that had been isolated from the faeces of Tibetan antelopes at the Hoh Xil Nature Reserve, Qinghai-Tibet Plateau, China.
30362934	2	61	theme	bacterial	159:167	arg1	601					186:188	601	186:188	601	186:188	In the present study, we describe two novel Gram-stain-positive, irregular rod-shaped bacterial strains, 78T and 601, that had been isolated from the faeces of Tibetan antelopes at the Hoh Xil Nature Reserve, Qinghai-Tibet Plateau, China.
30362934	2	61	theme	bacterial	159:167	arg1	78T					178:180	78T	178:180	78T	178:180	In the present study, we describe two novel Gram-stain-positive, irregular rod-shaped bacterial strains, 78T and 601, that had been isolated from the faeces of Tibetan antelopes at the Hoh Xil Nature Reserve, Qinghai-Tibet Plateau, China.
30362934	6	62	theme	nucleotide	836:845	arg1	values					856:861	The average nucleotide identity values	824:861	The average nucleotide identity values between the two novel strains and the three closely related type strains of the genus Nocardioides	824:960	The average nucleotide identity values between the two novel strains and the three closely related type strains of the genus Nocardioides were lower than the 95-96 % threshold.
30362934	6	62	theme	nucleotide	836:845	arg1	lower					967:971	lower	967:971	lower	967:971	The average nucleotide identity values between the two novel strains and the three closely related type strains of the genus Nocardioides were lower than the 95-96 % threshold.
30362934	11	63	theme	strains	1560:1566	arg1	affiliation					1545:1555	the affiliation	1541:1555	the affiliation of strains 78T and 601 to genus Nocardioides	1541:1600	These data supported the affiliation of strains 78T and 601 to genus Nocardioides.
30362934	12	64	theme	Nocardioideshouyundeii	1722:1743	arg1	sp					1745:1746	Nocardioideshouyundeii sp	1722:1746	Nocardioideshouyundeii sp	1722:1746	Based on evidence collected from the phenotypic, genotypic and phylogenetic analyses, we propose a novel species named Nocardioideshouyundeii sp.
30362934	8	65	from	acid	1190:1193	arg1	peptidoglycan					1212:1224	its cell-wall peptidoglycan	1198:1224	its cell-wall peptidoglycan	1198:1224	MK-8 (H4) was the predominant respiratory quinone and ll-2,6-diaminopimelic acid was the diagnostic diamino acid in its cell-wall peptidoglycan.
30362934	2	66	theme	Qinghai-Tibet	282:294	arg1	Plateau					296:302	Qinghai-Tibet Plateau	282:302	Qinghai-Tibet Plateau	282:302	In the present study, we describe two novel Gram-stain-positive, irregular rod-shaped bacterial strains, 78T and 601, that had been isolated from the faeces of Tibetan antelopes at the Hoh Xil Nature Reserve, Qinghai-Tibet Plateau, China.
30362934	2	66	theme	Qinghai-Tibet	282:294	arg1	antelopes					241:249	antelopes	241:249	antelopes	241:249	In the present study, we describe two novel Gram-stain-positive, irregular rod-shaped bacterial strains, 78T and 601, that had been isolated from the faeces of Tibetan antelopes at the Hoh Xil Nature Reserve, Qinghai-Tibet Plateau, China.
30362934	11	67	dep	strains	1560:1566	arg1	601					1576:1578	601	1576:1578	601	1576:1578	These data supported the affiliation of strains 78T and 601 to genus Nocardioides.
30362934	11	67	dep	strains	1560:1566	arg1	78T					1568:1570	78T	1568:1570	78T	1568:1570	These data supported the affiliation of strains 78T and 601 to genus Nocardioides.
30362934	14	68	theme	4.7461T=DSM	1785:1795	arg1	106424T					1797:1803	=CGMCC 4.7461T=DSM 106424T	1778:1803	=CGMCC 4.7461T=DSM 106424T	1778:1803	The type strain is 78T (=CGMCC 4.7461T=DSM 106424T).
30362934	14	68	theme	4.7461T=DSM	1785:1795	arg1	78T					1773:1775	78T	1773:1775	78T (=CGMCC 4.7461T=DSM 106424T)	1773:1804	The type strain is 78T (=CGMCC 4.7461T=DSM 106424T).
30362934	4	69	theme	infusion	405:412	arg1	agar					414:417	brain-heart infusion agar	393:417	brain-heart infusion agar supplemented with 5 % sheep blood	393:451	When cultured on brain-heart infusion agar supplemented with 5 % sheep blood, colonies were cream in colour, circular, smooth and convex.
30362934	0	70	dep	sp	24:25	arg1	Nocardioides					0:11	Nocardioides	0:11	Nocardioides	0:11	Nocardioides houyundeii sp.
30362934	5	71	theme	Nocardioides	693:704	arg1	%					734:734	98.3 %	729:734	98.3 %	729:734	Phylogenetic analysis based on the full-length 16S rRNA sequences revealed that type strain 78T and strain 601 belong to the genus Nocardioides, sharing the highest similarity to Nocardioides solisilvae JCM 31492T (98.3 %), Nocardioides gilvus XZ17T (97.4 %) and Nocardioides daejeonensis JCM 16922T (97.4 %).
30362934	5	71	theme	Nocardioides	693:704	arg1	31492T					721:726	Nocardioides solisilvae JCM 31492T	693:726	Nocardioides solisilvae JCM 31492T (98.3 %)	693:735	Phylogenetic analysis based on the full-length 16S rRNA sequences revealed that type strain 78T and strain 601 belong to the genus Nocardioides, sharing the highest similarity to Nocardioides solisilvae JCM 31492T (98.3 %), Nocardioides gilvus XZ17T (97.4 %) and Nocardioides daejeonensis JCM 16922T (97.4 %).
30362934	4	72	theme	brain-heart	393:403	arg1	agar					414:417	brain-heart infusion agar	393:417	brain-heart infusion agar supplemented with 5 % sheep blood	393:451	When cultured on brain-heart infusion agar supplemented with 5 % sheep blood, colonies were cream in colour, circular, smooth and convex.
30362934	12	73	theme	genotypic	1652:1660	arg1	analyses					1679:1686	the phenotypic, genotypic and phylogenetic analyses	1636:1686	the phenotypic, genotypic and phylogenetic analyses	1636:1686	Based on evidence collected from the phenotypic, genotypic and phylogenetic analyses, we propose a novel species named Nocardioideshouyundeii sp.
30362934	8	74	theme	cell-wall	1202:1210	arg1	peptidoglycan					1212:1224	its cell-wall peptidoglycan	1198:1224	its cell-wall peptidoglycan	1198:1224	MK-8 (H4) was the predominant respiratory quinone and ll-2,6-diaminopimelic acid was the diagnostic diamino acid in its cell-wall peptidoglycan.
30362934	8	75	theme	predominant	1100:1110	arg1	quinone					1124:1130	the predominant respiratory quinone	1096:1130	the predominant respiratory quinone	1096:1130	MK-8 (H4) was the predominant respiratory quinone and ll-2,6-diaminopimelic acid was the diagnostic diamino acid in its cell-wall peptidoglycan.
30362934	8	75	theme	predominant	1100:1110	arg1	MK-8					1082:1085	MK-8	1082:1085	MK-8 (H4)	1082:1090	MK-8 (H4) was the predominant respiratory quinone and ll-2,6-diaminopimelic acid was the diagnostic diamino acid in its cell-wall peptidoglycan.
30362934	6	76	theme	type	923:926	arg1	strains					928:934	the three closely related type strains	897:934	the three closely related type strains of the genus Nocardioides	897:960	The average nucleotide identity values between the two novel strains and the three closely related type strains of the genus Nocardioides were lower than the 95-96 % threshold.
30362934	5	77	theme	solisilvae	706:715	arg1	%					734:734	98.3 %	729:734	98.3 %	729:734	Phylogenetic analysis based on the full-length 16S rRNA sequences revealed that type strain 78T and strain 601 belong to the genus Nocardioides, sharing the highest similarity to Nocardioides solisilvae JCM 31492T (98.3 %), Nocardioides gilvus XZ17T (97.4 %) and Nocardioides daejeonensis JCM 16922T (97.4 %).
30362934	5	77	theme	solisilvae	706:715	arg1	31492T					721:726	Nocardioides solisilvae JCM 31492T	693:726	Nocardioides solisilvae JCM 31492T (98.3 %)	693:735	Phylogenetic analysis based on the full-length 16S rRNA sequences revealed that type strain 78T and strain 601 belong to the genus Nocardioides, sharing the highest similarity to Nocardioides solisilvae JCM 31492T (98.3 %), Nocardioides gilvus XZ17T (97.4 %) and Nocardioides daejeonensis JCM 16922T (97.4 %).
30362934	6	78	theme	average	828:834	arg1	values					856:861	The average nucleotide identity values	824:861	The average nucleotide identity values between the two novel strains and the three closely related type strains of the genus Nocardioides	824:960	The average nucleotide identity values between the two novel strains and the three closely related type strains of the genus Nocardioides were lower than the 95-96 % threshold.
30362934	6	78	theme	average	828:834	arg1	lower					967:971	lower	967:971	lower	967:971	The average nucleotide identity values between the two novel strains and the three closely related type strains of the genus Nocardioides were lower than the 95-96 % threshold.
30362934	9	79	theme	unidentified	1325:1336	arg1	phospholipid					1338:1349	an unidentified phospholipid	1322:1349	an unidentified phospholipid	1322:1349	Its polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, an unidentified phospholipid and an unidentified lipid.
30362934	7	80	theme	71.3 mol	1058:1065	arg1	%					1066:1066	71.3 mol%	1058:1066	71.3 mol%	1058:1066	The DNA G+C content of strains 78T and 601 were 71.2 and 71.3 mol% respectively.
30362934	6	81	theme	related	915:921	arg1	strains					928:934	the three closely related type strains	897:934	the three closely related type strains of the genus Nocardioides	897:960	The average nucleotide identity values between the two novel strains and the three closely related type strains of the genus Nocardioides were lower than the 95-96 % threshold.
31310191	12	0	theme	sequence	1209:1216	arg1	analysis					1218:1225	the 16S rRNA gene sequence analysis	1191:1225	the 16S rRNA gene sequence analysis	1191:1225	According to the 16S rRNA gene sequence analysis, strain FBKL4.011T was closely related to Thermoflavimicrobium dichotomicum JCM 9688T (95.3 % sequence similarity), and other members within the family Thermoactinomycetaceae (less than 93.0 % sequence similarity).
31310191	2	1	theme	important	121:129	arg1	starter					131:137	an important starter to support growth of microorganisms in the fermented process of Moutai-flavour liquor	118:223	an important starter to support growth of microorganisms in the fermented process of Moutai-flavour liquor	118:223	Moutai-flavour Daqu is an important starter to support growth of microorganisms in the fermented process of Moutai-flavour liquor.
31310191	2	1	theme	important	121:129	arg1	Daqu					110:113	Moutai-flavour Daqu	95:113	Moutai-flavour Daqu	95:113	Moutai-flavour Daqu is an important starter to support growth of microorganisms in the fermented process of Moutai-flavour liquor.
31310191	3	2	theme	novel	228:232	arg1	microorganism					247:259	A novel thermophilic microorganism	226:259	A novel thermophilic microorganism	226:259	A novel thermophilic microorganism, designated strain FBKL4.011T, was isolated from Moutai-flavour Daqu samples collected from Guotai distillery in Renhuai, Guizhou province, south-west China.
31310191	5	3	theme	genus	540:544	arg1	Thermoflavimicrobium					546:565	the genus Thermoflavimicrobium	536:565	the genus Thermoflavimicrobium	536:565	Based on polyphasic analysis, strain FBKL4.011T was affiliated to the genus Thermoflavimicrobium.
31310191	13	4	theme	T.	1526:1527	arg1	9790T					1577:1581	Laceyella sacchari KCTC 9790T	1553:1581	Laceyella sacchari KCTC 9790T	1553:1581	The DNA-DNA hybridisation data showed low relatedness between strain FBKL4.011T and T. dichotomicum JCM 9688T, Laceyella sacchari KCTC 9790T, Laceyella tengchongensis YIM 10002T, Laceyella sediminis RHA1T(36.7±1.1 %, 30.0±1.2 %, 21.3±2.1 % % and 37.6±0.9 %, respectively).
31310191	13	4	theme	T.	1526:1527	arg1	9688T					1546:1550	T. dichotomicum JCM 9688T	1526:1550	T. dichotomicum JCM 9688T	1526:1550	The DNA-DNA hybridisation data showed low relatedness between strain FBKL4.011T and T. dichotomicum JCM 9688T, Laceyella sacchari KCTC 9790T, Laceyella tengchongensis YIM 10002T, Laceyella sediminis RHA1T(36.7±1.1 %, 30.0±1.2 %, 21.3±2.1 % % and 37.6±0.9 %, respectively).
31310191	13	4	theme	T.	1526:1527	arg1	RHA1T					1641:1645	RHA1T	1641:1645	RHA1T	1641:1645	The DNA-DNA hybridisation data showed low relatedness between strain FBKL4.011T and T. dichotomicum JCM 9688T, Laceyella sacchari KCTC 9790T, Laceyella tengchongensis YIM 10002T, Laceyella sediminis RHA1T(36.7±1.1 %, 30.0±1.2 %, 21.3±2.1 % % and 37.6±0.9 %, respectively).
31310191	12	5	theme	gene	1204:1207	arg1	analysis					1218:1225	the 16S rRNA gene sequence analysis	1191:1225	the 16S rRNA gene sequence analysis	1191:1225	According to the 16S rRNA gene sequence analysis, strain FBKL4.011T was closely related to Thermoflavimicrobium dichotomicum JCM 9688T (95.3 % sequence similarity), and other members within the family Thermoactinomycetaceae (less than 93.0 % sequence similarity).
31310191	10	6	theme	unidentified	1079:1090	arg1	phospholipid					1092:1103	one unidentified phospholipid	1075:1103	one unidentified phospholipid	1075:1103	The polar phospholipids contained diphosphatidyl glycerol, phosphatidyl ethanolamine, one unidentified phospholipid and one unidentified lipid.
31310191	3	7	theme	thermophilic	234:245	arg1	microorganism					247:259	A novel thermophilic microorganism	226:259	A novel thermophilic microorganism	226:259	A novel thermophilic microorganism, designated strain FBKL4.011T, was isolated from Moutai-flavour Daqu samples collected from Guotai distillery in Renhuai, Guizhou province, south-west China.
31310191	12	8	theme	strain	1228:1233	arg1	FBKL4.011T					1235:1244	strain FBKL4.011T	1228:1244	strain FBKL4.011T	1228:1244	According to the 16S rRNA gene sequence analysis, strain FBKL4.011T was closely related to Thermoflavimicrobium dichotomicum JCM 9688T (95.3 % sequence similarity), and other members within the family Thermoactinomycetaceae (less than 93.0 % sequence similarity).
31310191	7	9	theme	meso-diaminopimelic	765:783	arg1	acid					785:788	meso-diaminopimelic acid	765:788	meso-diaminopimelic acid	765:788	The cell-wall peptidoglycan contained meso-diaminopimelic acid; ribose, glucose and mannose were the primary whole-cell sugars.
31310191	13	10	theme	strain	1504:1509	arg1	FBKL4.011T					1511:1520	strain FBKL4.011T	1504:1520	strain FBKL4.011T	1504:1520	The DNA-DNA hybridisation data showed low relatedness between strain FBKL4.011T and T. dichotomicum JCM 9688T, Laceyella sacchari KCTC 9790T, Laceyella tengchongensis YIM 10002T, Laceyella sediminis RHA1T(36.7±1.1 %, 30.0±1.2 %, 21.3±2.1 % % and 37.6±0.9 %, respectively).
31310191	6	11	theme	single	638:643	arg1	endospores					645:654	single endospores	638:654	single endospores (7.0-10.0 µm diameter) on branched long sporophores (5.0 µm diameter)	638:724	It formed abundant pale-yellow aerial and substrate mycelium, bearing single endospores (7.0-10.0 µm diameter) on branched long sporophores (5.0 µm diameter).
31310191	6	11	theme	single	638:643	arg1	diameter					669:676	7.0-10.0 µm diameter	657:676	7.0-10.0 µm diameter	657:676	It formed abundant pale-yellow aerial and substrate mycelium, bearing single endospores (7.0-10.0 µm diameter) on branched long sporophores (5.0 µm diameter).
31310191	7	12	theme	cell-wall	731:739	arg1	peptidoglycan					741:753	The cell-wall peptidoglycan	727:753	The cell-wall peptidoglycan	727:753	The cell-wall peptidoglycan contained meso-diaminopimelic acid; ribose, glucose and mannose were the primary whole-cell sugars.
31310191	13	13	dep	9688T	1546:1550	arg1	%					1656:1656	36.7±1.1 %	1647:1656	36.7±1.1 %	1647:1656	The DNA-DNA hybridisation data showed low relatedness between strain FBKL4.011T and T. dichotomicum JCM 9688T, Laceyella sacchari KCTC 9790T, Laceyella tengchongensis YIM 10002T, Laceyella sediminis RHA1T(36.7±1.1 %, 30.0±1.2 %, 21.3±2.1 % % and 37.6±0.9 %, respectively).
31310191	13	13	dep	9688T	1546:1550	arg1	%					1668:1668	30.0±1.2 %	1659:1668	30.0±1.2 %	1659:1668	The DNA-DNA hybridisation data showed low relatedness between strain FBKL4.011T and T. dichotomicum JCM 9688T, Laceyella sacchari KCTC 9790T, Laceyella tengchongensis YIM 10002T, Laceyella sediminis RHA1T(36.7±1.1 %, 30.0±1.2 %, 21.3±2.1 % % and 37.6±0.9 %, respectively).
31310191	13	13	dep	9688T	1546:1550	arg1	%					1682:1682	21.3±2.1 % %	1671:1682	21.3±2.1 % %	1671:1682	The DNA-DNA hybridisation data showed low relatedness between strain FBKL4.011T and T. dichotomicum JCM 9688T, Laceyella sacchari KCTC 9790T, Laceyella tengchongensis YIM 10002T, Laceyella sediminis RHA1T(36.7±1.1 %, 30.0±1.2 %, 21.3±2.1 % % and 37.6±0.9 %, respectively).
31310191	13	13	dep	9688T	1546:1550	arg1	%					1697:1697	37.6±0.9 %	1688:1697	37.6±0.9 %	1688:1697	The DNA-DNA hybridisation data showed low relatedness between strain FBKL4.011T and T. dichotomicum JCM 9688T, Laceyella sacchari KCTC 9790T, Laceyella tengchongensis YIM 10002T, Laceyella sediminis RHA1T(36.7±1.1 %, 30.0±1.2 %, 21.3±2.1 % % and 37.6±0.9 %, respectively).
31310191	14	14	theme	novel	1806:1810	arg1	species					1812:1818	a novel species	1804:1818	a novel species	1804:1818	Based on data from the polyphasic analysis, strain FBKL4.011T is considered to represent a novel species of the genus Thermoflavimicrobium, for which the name Thermoflavimicrobium daqui sp.
31310191	10	15	dep	diphosphatidyl	1023:1036	arg1	glycerol					1038:1045	glycerol	1038:1045	glycerol	1038:1045	The polar phospholipids contained diphosphatidyl glycerol, phosphatidyl ethanolamine, one unidentified phospholipid and one unidentified lipid.
31310191	14	16	theme	strain	1759:1764	arg1	FBKL4.011T					1766:1775	strain FBKL4.011T	1759:1775	strain FBKL4.011T	1759:1775	Based on data from the polyphasic analysis, strain FBKL4.011T is considered to represent a novel species of the genus Thermoflavimicrobium, for which the name Thermoflavimicrobium daqui sp.
31310191	13	17	theme	%	1680:1680	arg1	%					1682:1682	21.3±2.1 % %	1671:1682	21.3±2.1 % %	1671:1682	The DNA-DNA hybridisation data showed low relatedness between strain FBKL4.011T and T. dichotomicum JCM 9688T, Laceyella sacchari KCTC 9790T, Laceyella tengchongensis YIM 10002T, Laceyella sediminis RHA1T(36.7±1.1 %, 30.0±1.2 %, 21.3±2.1 % % and 37.6±0.9 %, respectively).
31310191	13	18	dep	Laceyella	1553:1561	arg1	sacchari					1563:1570	sacchari	1563:1570	sacchari	1563:1570	The DNA-DNA hybridisation data showed low relatedness between strain FBKL4.011T and T. dichotomicum JCM 9688T, Laceyella sacchari KCTC 9790T, Laceyella tengchongensis YIM 10002T, Laceyella sediminis RHA1T(36.7±1.1 %, 30.0±1.2 %, 21.3±2.1 % % and 37.6±0.9 %, respectively).
31310191	12	19	theme	sequence	1420:1427	arg1	similarity					1429:1438	less than 93.0 % sequence similarity	1403:1438	less than 93.0 % sequence similarity	1403:1438	According to the 16S rRNA gene sequence analysis, strain FBKL4.011T was closely related to Thermoflavimicrobium dichotomicum JCM 9688T (95.3 % sequence similarity), and other members within the family Thermoactinomycetaceae (less than 93.0 % sequence similarity).
31310191	12	19	theme	sequence	1420:1427	arg1	Thermoactinomycetaceae					1379:1400	the family Thermoactinomycetaceae	1368:1400	the family Thermoactinomycetaceae (less than 93.0 % sequence similarity)	1368:1439	According to the 16S rRNA gene sequence analysis, strain FBKL4.011T was closely related to Thermoflavimicrobium dichotomicum JCM 9688T (95.3 % sequence similarity), and other members within the family Thermoactinomycetaceae (less than 93.0 % sequence similarity).
31310191	5	20	theme	strain	500:505	arg1	FBKL4.011T					507:516	strain FBKL4.011T	500:516	strain FBKL4.011T	500:516	Based on polyphasic analysis, strain FBKL4.011T was affiliated to the genus Thermoflavimicrobium.
31310191	12	21	theme	95.3 	1314:1318	arg1	%					1319:1319	%	1319:1319	%	1319:1319	According to the 16S rRNA gene sequence analysis, strain FBKL4.011T was closely related to Thermoflavimicrobium dichotomicum JCM 9688T (95.3 % sequence similarity), and other members within the family Thermoactinomycetaceae (less than 93.0 % sequence similarity).
31310191	3	22	theme	Renhuai	374:380	arg1	province					391:398	Renhuai, Guizhou province	374:398	province	391:398	A novel thermophilic microorganism, designated strain FBKL4.011T, was isolated from Moutai-flavour Daqu samples collected from Guotai distillery in Renhuai, Guizhou province, south-west China.
31310191	3	22	theme	Renhuai	374:380	arg1	China					412:416	south-west China	401:416	south-west China	401:416	A novel thermophilic microorganism, designated strain FBKL4.011T, was isolated from Moutai-flavour Daqu samples collected from Guotai distillery in Renhuai, Guizhou province, south-west China.
31310191	3	22	theme	Renhuai	374:380	arg1	Guizhou					383:389	Guizhou	383:389	Guizhou	383:389	A novel thermophilic microorganism, designated strain FBKL4.011T, was isolated from Moutai-flavour Daqu samples collected from Guotai distillery in Renhuai, Guizhou province, south-west China.
31310191	10	23	theme	unidentified	1113:1124	arg1	lipid					1126:1130	one unidentified lipid	1109:1130	one unidentified lipid	1109:1130	The polar phospholipids contained diphosphatidyl glycerol, phosphatidyl ethanolamine, one unidentified phospholipid and one unidentified lipid.
31310191	3	24	theme	strain	273:278	arg1	FBKL4.011T					280:289	strain FBKL4.011T	273:289	strain FBKL4.011T	273:289	A novel thermophilic microorganism, designated strain FBKL4.011T, was isolated from Moutai-flavour Daqu samples collected from Guotai distillery in Renhuai, Guizhou province, south-west China.
31310191	16	25	theme	=KCTC	1953:1957	arg1	FBKL4.011T					1942:1951	FBKL4.011T	1942:1951	FBKL4.011T(=KCTC 43036T=CICC 24504T)	1942:1977	The type strain is FBKL4.011T(=KCTC 43036T=CICC 24504T).
31310191	16	25	theme	=KCTC	1953:1957	arg1	24504T					1971:1976	=KCTC 43036T=CICC 24504T	1953:1976	=KCTC 43036T=CICC 24504T	1953:1976	The type strain is FBKL4.011T(=KCTC 43036T=CICC 24504T).
31310191	11	26	theme	43.1 mol	1167:1174	arg1	G+C content					1137:1147	The G+C content	1133:1147	The G+C content of the genome	1133:1161	The G+C content of the genome was 43.1 mol%.
31310191	11	26	theme	43.1 mol	1167:1174	arg1	%					1175:1175	43.1 mol%	1167:1175	43.1 mol%	1167:1175	The G+C content of the genome was 43.1 mol%.
31310191	0	27	theme	daqui	21:25	arg1	sp					27:28	Thermoflavimicrobium daqui sp	0:28	Thermoflavimicrobium daqui sp.	0:29	Thermoflavimicrobium daqui sp.
31310191	12	28	dep	similarity	1429:1438	arg1	%					1418:1418	%	1418:1418	%	1418:1418	According to the 16S rRNA gene sequence analysis, strain FBKL4.011T was closely related to Thermoflavimicrobium dichotomicum JCM 9688T (95.3 % sequence similarity), and other members within the family Thermoactinomycetaceae (less than 93.0 % sequence similarity).
31310191	14	29	theme	Thermoflavimicrobium	1833:1852	arg1	species					1812:1818	a novel species	1804:1818	a novel species	1804:1818	Based on data from the polyphasic analysis, strain FBKL4.011T is considered to represent a novel species of the genus Thermoflavimicrobium, for which the name Thermoflavimicrobium daqui sp.
31310191	12	30	theme	sequence	1321:1328	arg1	similarity					1330:1339	95.3 % sequence similarity	1314:1339	95.3 % sequence similarity	1314:1339	According to the 16S rRNA gene sequence analysis, strain FBKL4.011T was closely related to Thermoflavimicrobium dichotomicum JCM 9688T (95.3 % sequence similarity), and other members within the family Thermoactinomycetaceae (less than 93.0 % sequence similarity).
31310191	0	31	theme	Thermoflavimicrobium	0:19	arg1	sp					27:28	Thermoflavimicrobium daqui sp	0:28	Thermoflavimicrobium daqui sp.	0:29	Thermoflavimicrobium daqui sp.
31310191	7	32	theme	whole-cell	836:845	arg1	mannose					811:817	mannose	811:817	mannose	811:817	The cell-wall peptidoglycan contained meso-diaminopimelic acid; ribose, glucose and mannose were the primary whole-cell sugars.
31310191	7	32	theme	whole-cell	836:845	arg1	glucose					799:805	glucose	799:805	glucose	799:805	The cell-wall peptidoglycan contained meso-diaminopimelic acid; ribose, glucose and mannose were the primary whole-cell sugars.
31310191	7	32	theme	whole-cell	836:845	arg1	ribose					791:796	ribose	791:796	ribose	791:796	The cell-wall peptidoglycan contained meso-diaminopimelic acid; ribose, glucose and mannose were the primary whole-cell sugars.
31310191	7	32	theme	whole-cell	836:845	arg1	sugars					847:852	the primary whole-cell sugars	824:852	the primary whole-cell sugars	824:852	The cell-wall peptidoglycan contained meso-diaminopimelic acid; ribose, glucose and mannose were the primary whole-cell sugars.
31310191	12	33	theme	family	1372:1377	arg1	similarity					1429:1438	less than 93.0 % sequence similarity	1403:1438	less than 93.0 % sequence similarity	1403:1438	According to the 16S rRNA gene sequence analysis, strain FBKL4.011T was closely related to Thermoflavimicrobium dichotomicum JCM 9688T (95.3 % sequence similarity), and other members within the family Thermoactinomycetaceae (less than 93.0 % sequence similarity).
31310191	12	33	theme	family	1372:1377	arg1	Thermoactinomycetaceae					1379:1400	the family Thermoactinomycetaceae	1368:1400	the family Thermoactinomycetaceae (less than 93.0 % sequence similarity)	1368:1439	According to the 16S rRNA gene sequence analysis, strain FBKL4.011T was closely related to Thermoflavimicrobium dichotomicum JCM 9688T (95.3 % sequence similarity), and other members within the family Thermoactinomycetaceae (less than 93.0 % sequence similarity).
31310191	12	34	theme	%	1319:1319	arg1	similarity					1330:1339	95.3 % sequence similarity	1314:1339	95.3 % sequence similarity	1314:1339	According to the 16S rRNA gene sequence analysis, strain FBKL4.011T was closely related to Thermoflavimicrobium dichotomicum JCM 9688T (95.3 % sequence similarity), and other members within the family Thermoactinomycetaceae (less than 93.0 % sequence similarity).
31310191	14	35	theme	daqui	1895:1899	arg1	sp					1901:1902	the name Thermoflavimicrobium daqui sp	1865:1902	the name Thermoflavimicrobium daqui sp	1865:1902	Based on data from the polyphasic analysis, strain FBKL4.011T is considered to represent a novel species of the genus Thermoflavimicrobium, for which the name Thermoflavimicrobium daqui sp.
31310191	10	36	theme	polar	993:997	arg1	phospholipids					999:1011	The polar phospholipids	989:1011	The polar phospholipids	989:1011	The polar phospholipids contained diphosphatidyl glycerol, phosphatidyl ethanolamine, one unidentified phospholipid and one unidentified lipid.
31310191	12	37	dep	Thermoflavimicrobium	1269:1288	arg1	dichotomicum					1290:1301	dichotomicum	1290:1301	dichotomicum	1290:1301	According to the 16S rRNA gene sequence analysis, strain FBKL4.011T was closely related to Thermoflavimicrobium dichotomicum JCM 9688T (95.3 % sequence similarity), and other members within the family Thermoactinomycetaceae (less than 93.0 % sequence similarity).
31310191	13	38	theme	low	1480:1482	arg1	relatedness					1484:1494	low relatedness	1480:1494	low relatedness between strain FBKL4.011T and T. dichotomicum JCM 9688T, Laceyella sacchari KCTC 9790T, Laceyella tengchongensis YIM 10002T, Laceyella sediminis RHA1T(36.7±1.1 %, 30.0±1.2 %, 21.3±2.1 % % and 37.6±0.9 %, respectively)	1480:1712	The DNA-DNA hybridisation data showed low relatedness between strain FBKL4.011T and T. dichotomicum JCM 9688T, Laceyella sacchari KCTC 9790T, Laceyella tengchongensis YIM 10002T, Laceyella sediminis RHA1T(36.7±1.1 %, 30.0±1.2 %, 21.3±2.1 % % and 37.6±0.9 %, respectively).
31310191	6	39	theme	substrate	610:618	arg1	mycelium					620:627	abundant pale-yellow aerial and substrate mycelium	578:627	mycelium	620:627	It formed abundant pale-yellow aerial and substrate mycelium, bearing single endospores (7.0-10.0 µm diameter) on branched long sporophores (5.0 µm diameter).
31310191	16	40	theme	43036T=CICC	1959:1969	arg1	FBKL4.011T					1942:1951	FBKL4.011T	1942:1951	FBKL4.011T(=KCTC 43036T=CICC 24504T)	1942:1977	The type strain is FBKL4.011T(=KCTC 43036T=CICC 24504T).
31310191	16	40	theme	43036T=CICC	1959:1969	arg1	24504T					1971:1976	=KCTC 43036T=CICC 24504T	1953:1976	=KCTC 43036T=CICC 24504T	1953:1976	The type strain is FBKL4.011T(=KCTC 43036T=CICC 24504T).
31310191	2	41	theme	fermented	182:190	arg1	process					192:198	the fermented process	178:198	the fermented process of Moutai-flavour liquor	178:223	Moutai-flavour Daqu is an important starter to support growth of microorganisms in the fermented process of Moutai-flavour liquor.
31310191	5	42	theme	polyphasic	479:488	arg1	analysis					490:497	polyphasic analysis	479:497	polyphasic analysis	479:497	Based on polyphasic analysis, strain FBKL4.011T was affiliated to the genus Thermoflavimicrobium.
31310191	12	43	theme	Thermoflavimicrobium	1269:1288	arg1	9688T					1307:1311	Thermoflavimicrobium dichotomicum JCM 9688T	1269:1311	Thermoflavimicrobium dichotomicum JCM 9688T	1269:1311	According to the 16S rRNA gene sequence analysis, strain FBKL4.011T was closely related to Thermoflavimicrobium dichotomicum JCM 9688T (95.3 % sequence similarity), and other members within the family Thermoactinomycetaceae (less than 93.0 % sequence similarity).
31310191	7	44	theme	primary	828:834	arg1	mannose					811:817	mannose	811:817	mannose	811:817	The cell-wall peptidoglycan contained meso-diaminopimelic acid; ribose, glucose and mannose were the primary whole-cell sugars.
31310191	7	44	theme	primary	828:834	arg1	glucose					799:805	glucose	799:805	glucose	799:805	The cell-wall peptidoglycan contained meso-diaminopimelic acid; ribose, glucose and mannose were the primary whole-cell sugars.
31310191	7	44	theme	primary	828:834	arg1	ribose					791:796	ribose	791:796	ribose	791:796	The cell-wall peptidoglycan contained meso-diaminopimelic acid; ribose, glucose and mannose were the primary whole-cell sugars.
31310191	7	44	theme	primary	828:834	arg1	sugars					847:852	the primary whole-cell sugars	824:852	the primary whole-cell sugars	824:852	The cell-wall peptidoglycan contained meso-diaminopimelic acid; ribose, glucose and mannose were the primary whole-cell sugars.
31310191	4	45	theme	optimum	454:460	arg1	45-65 °C					444:451	45-65 °C	444:451	45-65 °C (optimum 45 °C)	444:467	The strain could grow at 45-65 °C (optimum 45 °C).
31310191	4	45	theme	optimum	454:460	arg1	45 °C					462:466	optimum 45 °C	454:466	optimum 45 °C	454:466	The strain could grow at 45-65 °C (optimum 45 °C).
31310191	13	46	dep	T.	1526:1527	arg1	dichotomicum					1529:1540	dichotomicum	1529:1540	dichotomicum	1529:1540	The DNA-DNA hybridisation data showed low relatedness between strain FBKL4.011T and T. dichotomicum JCM 9688T, Laceyella sacchari KCTC 9790T, Laceyella tengchongensis YIM 10002T, Laceyella sediminis RHA1T(36.7±1.1 %, 30.0±1.2 %, 21.3±2.1 % % and 37.6±0.9 %, respectively).
31310191	6	47	theme	long	691:694	arg1	sporophores					696:706	branched long sporophores	682:706	branched long sporophores (5.0 µm diameter)	682:724	It formed abundant pale-yellow aerial and substrate mycelium, bearing single endospores (7.0-10.0 µm diameter) on branched long sporophores (5.0 µm diameter).
31310191	6	47	theme	long	691:694	arg1	diameter					716:723	5.0 µm diameter	709:723	5.0 µm diameter	709:723	It formed abundant pale-yellow aerial and substrate mycelium, bearing single endospores (7.0-10.0 µm diameter) on branched long sporophores (5.0 µm diameter).
31310191	14	48	theme	Thermoflavimicrobium	1874:1893	arg1	sp					1901:1902	the name Thermoflavimicrobium daqui sp	1865:1902	the name Thermoflavimicrobium daqui sp	1865:1902	Based on data from the polyphasic analysis, strain FBKL4.011T is considered to represent a novel species of the genus Thermoflavimicrobium, for which the name Thermoflavimicrobium daqui sp.
31310191	14	49	from	analysis	1749:1756	arg1	data					1724:1727	data	1724:1727	data from the polyphasic analysis	1724:1756	Based on data from the polyphasic analysis, strain FBKL4.011T is considered to represent a novel species of the genus Thermoflavimicrobium, for which the name Thermoflavimicrobium daqui sp.
31310191	3	50	attach	isolated	296:303	arg2	microorganism					247:259	A novel thermophilic microorganism	226:259	A novel thermophilic microorganism	226:259	A novel thermophilic microorganism, designated strain FBKL4.011T, was isolated from Moutai-flavour Daqu samples collected from Guotai distillery in Renhuai, Guizhou province, south-west China.
31310191	3	50	attach	isolated	296:303	arg1	samples					330:336	Moutai-flavour Daqu samples	310:336	Moutai-flavour Daqu samples collected from Guotai distillery in Renhuai, Guizhou province, south-west China	310:416	A novel thermophilic microorganism, designated strain FBKL4.011T, was isolated from Moutai-flavour Daqu samples collected from Guotai distillery in Renhuai, Guizhou province, south-west China.
31310191	13	51	theme	21.3±2.1 	1671:1679	arg1	%					1682:1682	21.3±2.1 % %	1671:1682	21.3±2.1 % %	1671:1682	The DNA-DNA hybridisation data showed low relatedness between strain FBKL4.011T and T. dichotomicum JCM 9688T, Laceyella sacchari KCTC 9790T, Laceyella tengchongensis YIM 10002T, Laceyella sediminis RHA1T(36.7±1.1 %, 30.0±1.2 %, 21.3±2.1 % % and 37.6±0.9 %, respectively).
31310191	12	52	theme	other	1347:1351	arg1	members					1353:1359	other members	1347:1359	other members within the family Thermoactinomycetaceae (less than 93.0 % sequence similarity)	1347:1439	According to the 16S rRNA gene sequence analysis, strain FBKL4.011T was closely related to Thermoflavimicrobium dichotomicum JCM 9688T (95.3 % sequence similarity), and other members within the family Thermoactinomycetaceae (less than 93.0 % sequence similarity).
31310191	9	53	theme	predominant	945:955	arg1	MK-8					974:977	MK-8	974:977	MK-8	974:977	The predominant menaquinone were MK-8 and MK-9.
31310191	9	53	theme	predominant	945:955	arg1	menaquinone					957:967	The predominant menaquinone	941:967	The predominant menaquinone	941:967	The predominant menaquinone were MK-8 and MK-9.
31310191	2	54	theme	Moutai-flavour	95:108	arg1	starter					131:137	an important starter to support growth of microorganisms in the fermented process of Moutai-flavour liquor	118:223	an important starter to support growth of microorganisms in the fermented process of Moutai-flavour liquor	118:223	Moutai-flavour Daqu is an important starter to support growth of microorganisms in the fermented process of Moutai-flavour liquor.
31310191	2	54	theme	Moutai-flavour	95:108	arg1	Daqu					110:113	Moutai-flavour Daqu	95:113	Moutai-flavour Daqu	95:113	Moutai-flavour Daqu is an important starter to support growth of microorganisms in the fermented process of Moutai-flavour liquor.
31310191	3	55	theme	Daqu	325:328	arg1	samples					330:336	Moutai-flavour Daqu samples	310:336	Moutai-flavour Daqu samples collected from Guotai distillery in Renhuai, Guizhou province, south-west China	310:416	A novel thermophilic microorganism, designated strain FBKL4.011T, was isolated from Moutai-flavour Daqu samples collected from Guotai distillery in Renhuai, Guizhou province, south-west China.
31310191	2	56	theme	microorganisms	160:173	arg1	growth					150:155	growth	150:155	growth of microorganisms	150:173	Moutai-flavour Daqu is an important starter to support growth of microorganisms in the fermented process of Moutai-flavour liquor.
31310191	8	57	dep	iso-C14 	882:889	arg1	iso-C16 					928:935	iso-C16 	928:935	iso-C16 	928:935	The major fatty acids were iso-C14 : 0, iso-C15 : 0, anteiso-C15 : 0 and iso-C16 : 0.
31310191	8	57	dep	iso-C14 	882:889	arg1	 0					904:905	 0	904:905	 0	904:905	The major fatty acids were iso-C14 : 0, iso-C15 : 0, anteiso-C15 : 0 and iso-C16 : 0.
31310191	8	57	dep	iso-C14 	882:889	arg1	 0					921:922	 0	921:922	 0	921:922	The major fatty acids were iso-C14 : 0, iso-C15 : 0, anteiso-C15 : 0 and iso-C16 : 0.
31310191	8	57	dep	iso-C14 	882:889	arg1	 0					891:892	 0	891:892	 0	891:892	The major fatty acids were iso-C14 : 0, iso-C15 : 0, anteiso-C15 : 0 and iso-C16 : 0.
31310191	8	57	dep	iso-C14 	882:889	arg1	 0					937:938	 0	937:938	 0	937:938	The major fatty acids were iso-C14 : 0, iso-C15 : 0, anteiso-C15 : 0 and iso-C16 : 0.
31310191	13	58	theme	DNA-DNA	1446:1452	arg1	data					1468:1471	The DNA-DNA hybridisation data	1442:1471	The DNA-DNA hybridisation data	1442:1471	The DNA-DNA hybridisation data showed low relatedness between strain FBKL4.011T and T. dichotomicum JCM 9688T, Laceyella sacchari KCTC 9790T, Laceyella tengchongensis YIM 10002T, Laceyella sediminis RHA1T(36.7±1.1 %, 30.0±1.2 %, 21.3±2.1 % % and 37.6±0.9 %, respectively).
31310191	2	59	theme	liquor	218:223	arg1	process					192:198	the fermented process	178:198	the fermented process of Moutai-flavour liquor	178:223	Moutai-flavour Daqu is an important starter to support growth of microorganisms in the fermented process of Moutai-flavour liquor.
31310191	7	60	contain	contained	755:763	arg1	peptidoglycan					741:753	The cell-wall peptidoglycan	727:753	The cell-wall peptidoglycan	727:753	The cell-wall peptidoglycan contained meso-diaminopimelic acid; ribose, glucose and mannose were the primary whole-cell sugars.
31310191	7	60	contain	contained	755:763	arg2	acid					785:788	meso-diaminopimelic acid	765:788	meso-diaminopimelic acid	765:788	The cell-wall peptidoglycan contained meso-diaminopimelic acid; ribose, glucose and mannose were the primary whole-cell sugars.
31310191	13	61	theme	hybridisation	1454:1466	arg1	data					1468:1471	The DNA-DNA hybridisation data	1442:1471	The DNA-DNA hybridisation data	1442:1471	The DNA-DNA hybridisation data showed low relatedness between strain FBKL4.011T and T. dichotomicum JCM 9688T, Laceyella sacchari KCTC 9790T, Laceyella tengchongensis YIM 10002T, Laceyella sediminis RHA1T(36.7±1.1 %, 30.0±1.2 %, 21.3±2.1 % % and 37.6±0.9 %, respectively).
31310191	6	62	theme	branched	682:689	arg1	sporophores					696:706	branched long sporophores	682:706	branched long sporophores (5.0 µm diameter)	682:724	It formed abundant pale-yellow aerial and substrate mycelium, bearing single endospores (7.0-10.0 µm diameter) on branched long sporophores (5.0 µm diameter).
31310191	6	62	theme	branched	682:689	arg1	diameter					716:723	5.0 µm diameter	709:723	5.0 µm diameter	709:723	It formed abundant pale-yellow aerial and substrate mycelium, bearing single endospores (7.0-10.0 µm diameter) on branched long sporophores (5.0 µm diameter).
31310191	13	63	theme	Laceyella	1553:1561	arg1	9790T					1577:1581	Laceyella sacchari KCTC 9790T	1553:1581	Laceyella sacchari KCTC 9790T	1553:1581	The DNA-DNA hybridisation data showed low relatedness between strain FBKL4.011T and T. dichotomicum JCM 9688T, Laceyella sacchari KCTC 9790T, Laceyella tengchongensis YIM 10002T, Laceyella sediminis RHA1T(36.7±1.1 %, 30.0±1.2 %, 21.3±2.1 % % and 37.6±0.9 %, respectively).
31310191	13	63	theme	Laceyella	1553:1561	arg1	9688T					1546:1550	T. dichotomicum JCM 9688T	1526:1550	T. dichotomicum JCM 9688T	1526:1550	The DNA-DNA hybridisation data showed low relatedness between strain FBKL4.011T and T. dichotomicum JCM 9688T, Laceyella sacchari KCTC 9790T, Laceyella tengchongensis YIM 10002T, Laceyella sediminis RHA1T(36.7±1.1 %, 30.0±1.2 %, 21.3±2.1 % % and 37.6±0.9 %, respectively).
31310191	2	64	theme	Moutai-flavour	203:216	arg1	liquor					218:223	Moutai-flavour liquor	203:223	Moutai-flavour liquor	203:223	Moutai-flavour Daqu is an important starter to support growth of microorganisms in the fermented process of Moutai-flavour liquor.
31310191	6	65	from	endospores	645:654	arg1	sporophores					696:706	branched long sporophores	682:706	branched long sporophores (5.0 µm diameter)	682:724	It formed abundant pale-yellow aerial and substrate mycelium, bearing single endospores (7.0-10.0 µm diameter) on branched long sporophores (5.0 µm diameter).
31310191	6	65	from	endospores	645:654	arg1	diameter					716:723	5.0 µm diameter	709:723	5.0 µm diameter	709:723	It formed abundant pale-yellow aerial and substrate mycelium, bearing single endospores (7.0-10.0 µm diameter) on branched long sporophores (5.0 µm diameter).
31310191	13	66	theme	KCTC	1572:1575	arg1	9790T					1577:1581	Laceyella sacchari KCTC 9790T	1553:1581	Laceyella sacchari KCTC 9790T	1553:1581	The DNA-DNA hybridisation data showed low relatedness between strain FBKL4.011T and T. dichotomicum JCM 9688T, Laceyella sacchari KCTC 9790T, Laceyella tengchongensis YIM 10002T, Laceyella sediminis RHA1T(36.7±1.1 %, 30.0±1.2 %, 21.3±2.1 % % and 37.6±0.9 %, respectively).
31310191	13	66	theme	KCTC	1572:1575	arg1	9688T					1546:1550	T. dichotomicum JCM 9688T	1526:1550	T. dichotomicum JCM 9688T	1526:1550	The DNA-DNA hybridisation data showed low relatedness between strain FBKL4.011T and T. dichotomicum JCM 9688T, Laceyella sacchari KCTC 9790T, Laceyella tengchongensis YIM 10002T, Laceyella sediminis RHA1T(36.7±1.1 %, 30.0±1.2 %, 21.3±2.1 % % and 37.6±0.9 %, respectively).
31310191	14	67	theme	genus	1827:1831	arg1	Thermoflavimicrobium					1833:1852	the genus Thermoflavimicrobium	1823:1852	the genus Thermoflavimicrobium	1823:1852	Based on data from the polyphasic analysis, strain FBKL4.011T is considered to represent a novel species of the genus Thermoflavimicrobium, for which the name Thermoflavimicrobium daqui sp.
31310191	10	68	contain	contained	1013:1021	arg2	ethanolamine					1061:1072	phosphatidyl ethanolamine	1048:1072	phosphatidyl ethanolamine	1048:1072	The polar phospholipids contained diphosphatidyl glycerol, phosphatidyl ethanolamine, one unidentified phospholipid and one unidentified lipid.
31310191	10	68	contain	contained	1013:1021	arg1	phospholipids					999:1011	The polar phospholipids	989:1011	The polar phospholipids	989:1011	The polar phospholipids contained diphosphatidyl glycerol, phosphatidyl ethanolamine, one unidentified phospholipid and one unidentified lipid.
31310191	10	68	contain	contained	1013:1021	arg2	lipid					1126:1130	one unidentified lipid	1109:1130	one unidentified lipid	1109:1130	The polar phospholipids contained diphosphatidyl glycerol, phosphatidyl ethanolamine, one unidentified phospholipid and one unidentified lipid.
31310191	10	68	contain	contained	1013:1021	arg2	phospholipid					1092:1103	one unidentified phospholipid	1075:1103	one unidentified phospholipid	1075:1103	The polar phospholipids contained diphosphatidyl glycerol, phosphatidyl ethanolamine, one unidentified phospholipid and one unidentified lipid.
31310191	10	68	contain	contained	1013:1021	arg2	diphosphatidyl					1023:1036	diphosphatidyl glycerol, phosphatidyl ethanolamine, one unidentified phospholipid and one unidentified lipid	1023:1130	diphosphatidyl	1023:1036	The polar phospholipids contained diphosphatidyl glycerol, phosphatidyl ethanolamine, one unidentified phospholipid and one unidentified lipid.
31310191	14	69	theme	polyphasic	1738:1747	arg1	analysis					1749:1756	the polyphasic analysis	1734:1756	the polyphasic analysis	1734:1756	Based on data from the polyphasic analysis, strain FBKL4.011T is considered to represent a novel species of the genus Thermoflavimicrobium, for which the name Thermoflavimicrobium daqui sp.
31310191	3	70	theme	Moutai-flavour	310:323	arg1	samples					330:336	Moutai-flavour Daqu samples	310:336	Moutai-flavour Daqu samples collected from Guotai distillery in Renhuai, Guizhou province, south-west China	310:416	A novel thermophilic microorganism, designated strain FBKL4.011T, was isolated from Moutai-flavour Daqu samples collected from Guotai distillery in Renhuai, Guizhou province, south-west China.
31310191	10	71	theme	phosphatidyl	1048:1059	arg1	ethanolamine					1061:1072	phosphatidyl ethanolamine	1048:1072	phosphatidyl ethanolamine	1048:1072	The polar phospholipids contained diphosphatidyl glycerol, phosphatidyl ethanolamine, one unidentified phospholipid and one unidentified lipid.
31310191	12	72	dep	%	1418:1418	arg1	93.0 					1413:1417	93.0 	1413:1417	93.0 	1413:1417	According to the 16S rRNA gene sequence analysis, strain FBKL4.011T was closely related to Thermoflavimicrobium dichotomicum JCM 9688T (95.3 % sequence similarity), and other members within the family Thermoactinomycetaceae (less than 93.0 % sequence similarity).
31310191	8	73	theme	fatty	865:869	arg1	iso-C14 					882:889	iso-C14 	882:889	iso-C14 	882:889	The major fatty acids were iso-C14 : 0, iso-C15 : 0, anteiso-C15 : 0 and iso-C16 : 0.
31310191	8	73	theme	fatty	865:869	arg1	acids					871:875	The major fatty acids	855:875	The major fatty acids	855:875	The major fatty acids were iso-C14 : 0, iso-C15 : 0, anteiso-C15 : 0 and iso-C16 : 0.
31310191	16	74	theme	type	1927:1930	arg1	FBKL4.011T					1942:1951	FBKL4.011T	1942:1951	FBKL4.011T(=KCTC 43036T=CICC 24504T)	1942:1977	The type strain is FBKL4.011T(=KCTC 43036T=CICC 24504T).
31310191	16	74	theme	type	1927:1930	arg1	strain					1932:1937	The type strain	1923:1937	The type strain	1923:1937	The type strain is FBKL4.011T(=KCTC 43036T=CICC 24504T).
31310191	12	75	theme	rRNA	1199:1202	arg1	analysis					1218:1225	the 16S rRNA gene sequence analysis	1191:1225	the 16S rRNA gene sequence analysis	1191:1225	According to the 16S rRNA gene sequence analysis, strain FBKL4.011T was closely related to Thermoflavimicrobium dichotomicum JCM 9688T (95.3 % sequence similarity), and other members within the family Thermoactinomycetaceae (less than 93.0 % sequence similarity).
31310191	12	76	theme	JCM	1303:1305	arg1	9688T					1307:1311	Thermoflavimicrobium dichotomicum JCM 9688T	1269:1311	Thermoflavimicrobium dichotomicum JCM 9688T	1269:1311	According to the 16S rRNA gene sequence analysis, strain FBKL4.011T was closely related to Thermoflavimicrobium dichotomicum JCM 9688T (95.3 % sequence similarity), and other members within the family Thermoactinomycetaceae (less than 93.0 % sequence similarity).
31310191	11	77	theme	genome	1156:1161	arg1	G+C content					1137:1147	The G+C content	1133:1147	The G+C content of the genome	1133:1161	The G+C content of the genome was 43.1 mol%.
31310191	11	77	theme	genome	1156:1161	arg1	%					1175:1175	43.1 mol%	1167:1175	43.1 mol%	1167:1175	The G+C content of the genome was 43.1 mol%.
31310191	14	78	theme	name	1869:1872	arg1	sp					1901:1902	the name Thermoflavimicrobium daqui sp	1865:1902	the name Thermoflavimicrobium daqui sp	1865:1902	Based on data from the polyphasic analysis, strain FBKL4.011T is considered to represent a novel species of the genus Thermoflavimicrobium, for which the name Thermoflavimicrobium daqui sp.
31310191	8	79	theme	major	859:863	arg1	iso-C14 					882:889	iso-C14 	882:889	iso-C14 	882:889	The major fatty acids were iso-C14 : 0, iso-C15 : 0, anteiso-C15 : 0 and iso-C16 : 0.
31310191	8	79	theme	major	859:863	arg1	acids					871:875	The major fatty acids	855:875	The major fatty acids	855:875	The major fatty acids were iso-C14 : 0, iso-C15 : 0, anteiso-C15 : 0 and iso-C16 : 0.
31310191	12	80	theme	16S	1195:1197	arg1	analysis					1218:1225	the 16S rRNA gene sequence analysis	1191:1225	the 16S rRNA gene sequence analysis	1191:1225	According to the 16S rRNA gene sequence analysis, strain FBKL4.011T was closely related to Thermoflavimicrobium dichotomicum JCM 9688T (95.3 % sequence similarity), and other members within the family Thermoactinomycetaceae (less than 93.0 % sequence similarity).
31310191	1	81	theme	thermophilic	39:50	arg1	microbe					52:58	a thermophilic microbe	37:58	a thermophilic microbe	37:58	nov., a thermophilic microbe isolated from Moutai-flavour Daqu.
31310191	1	81	theme	thermophilic	39:50	arg1	nov.					31:34	nov.	31:34	nov.	31:34	nov., a thermophilic microbe isolated from Moutai-flavour Daqu.
31310191	3	82	theme	south-west	401:410	arg1	province					391:398	Renhuai, Guizhou province	374:398	province	391:398	A novel thermophilic microorganism, designated strain FBKL4.011T, was isolated from Moutai-flavour Daqu samples collected from Guotai distillery in Renhuai, Guizhou province, south-west China.
31310191	3	82	theme	south-west	401:410	arg1	China					412:416	south-west China	401:416	south-west China	401:416	A novel thermophilic microorganism, designated strain FBKL4.011T, was isolated from Moutai-flavour Daqu samples collected from Guotai distillery in Renhuai, Guizhou province, south-west China.
31310191	6	83	theme	7.0-10.0 µm	657:667	arg1	endospores					645:654	single endospores	638:654	single endospores (7.0-10.0 µm diameter) on branched long sporophores (5.0 µm diameter)	638:724	It formed abundant pale-yellow aerial and substrate mycelium, bearing single endospores (7.0-10.0 µm diameter) on branched long sporophores (5.0 µm diameter).
31310191	6	83	theme	7.0-10.0 µm	657:667	arg1	diameter					669:676	7.0-10.0 µm diameter	657:676	7.0-10.0 µm diameter	657:676	It formed abundant pale-yellow aerial and substrate mycelium, bearing single endospores (7.0-10.0 µm diameter) on branched long sporophores (5.0 µm diameter).
31310191	13	84	theme	JCM	1542:1544	arg1	9790T					1577:1581	Laceyella sacchari KCTC 9790T	1553:1581	Laceyella sacchari KCTC 9790T	1553:1581	The DNA-DNA hybridisation data showed low relatedness between strain FBKL4.011T and T. dichotomicum JCM 9688T, Laceyella sacchari KCTC 9790T, Laceyella tengchongensis YIM 10002T, Laceyella sediminis RHA1T(36.7±1.1 %, 30.0±1.2 %, 21.3±2.1 % % and 37.6±0.9 %, respectively).
31310191	13	84	theme	JCM	1542:1544	arg1	9688T					1546:1550	T. dichotomicum JCM 9688T	1526:1550	T. dichotomicum JCM 9688T	1526:1550	The DNA-DNA hybridisation data showed low relatedness between strain FBKL4.011T and T. dichotomicum JCM 9688T, Laceyella sacchari KCTC 9790T, Laceyella tengchongensis YIM 10002T, Laceyella sediminis RHA1T(36.7±1.1 %, 30.0±1.2 %, 21.3±2.1 % % and 37.6±0.9 %, respectively).
31310191	13	84	theme	JCM	1542:1544	arg1	RHA1T					1641:1645	RHA1T	1641:1645	RHA1T	1641:1645	The DNA-DNA hybridisation data showed low relatedness between strain FBKL4.011T and T. dichotomicum JCM 9688T, Laceyella sacchari KCTC 9790T, Laceyella tengchongensis YIM 10002T, Laceyella sediminis RHA1T(36.7±1.1 %, 30.0±1.2 %, 21.3±2.1 % % and 37.6±0.9 %, respectively).
30202002	5	0	theme	components	710:719	arg1	list					702:705	the list	698:705	the list of components erroneously attributed to CA	698:748	Here we extend the list of components erroneously attributed to CA.
30202002	4	1	theme	tau	662:664	arg1	protein					666:672	tau protein	662:672	tau protein	662:672	We also noted that these neo-epitopes and IgMs were the cause of false staining in CA immunohistochemical studies, and disproved the proposed presence of β-amyloid peptides and tau protein in them.
30202002	6	2	theme	other	897:901	arg1	components					903:912	other components	897:912	other components	897:912	We show that, contrary to previous descriptions, CA do not contain GFAP, S100, AQP4, NeuN or class III β-tubulin, and we question the presence of other components.
30202002	4	3	from	staining	556:563	arg1	studies					591:597	CA immunohistochemical studies	568:597	CA immunohistochemical studies	568:597	We also noted that these neo-epitopes and IgMs were the cause of false staining in CA immunohistochemical studies, and disproved the proposed presence of β-amyloid peptides and tau protein in them.
30202002	1	4	theme	human	139:143	arg1	brain					145:149	the human brain	135:149	the human brain	135:149	Corpora amylacea (CA) are polyglucosan bodies that accumulate in the human brain during ageing and are also present in large numbers in neurodegenerative conditions.
30202002	3	5	theme	previous	332:339	arg1	work					341:344	previous work	332:344	previous work	332:344	In previous work, we revealed the presence of some neo-epitopes in CA and the existence of some natural IgM antibodies directed against these neo-epitopes.
30202002	3	6	from	neo-epitopes	380:391	arg1	CA					396:397	CA	396:397	CA	396:397	In previous work, we revealed the presence of some neo-epitopes in CA and the existence of some natural IgM antibodies directed against these neo-epitopes.
30202002	2	7	theme	CA	271:272	arg1	function					259:266	the function	255:266	the function of CA	255:272	Theories regarding the function of CA are regularly updated as new components are described.
30202002	4	8	theme	false	550:554	arg1	staining					556:563	false staining	550:563	false staining in CA immunohistochemical studies	550:597	We also noted that these neo-epitopes and IgMs were the cause of false staining in CA immunohistochemical studies, and disproved the proposed presence of β-amyloid peptides and tau protein in them.
30202002	7	9	theme	indispensable	1081:1093	arg1	synthase					1068:1075	glycogen synthase	1059:1075	glycogen synthase	1059:1075	Nonetheless, we observe that CA contains ubiquitin and p62, both of them associated with processes of elimination of waste substances, and also glycogen synthase, an indispensable enzyme for polyglucosan formation.
30202002	7	9	theme	indispensable	1081:1093	arg1	enzyme					1095:1100	an indispensable enzyme	1078:1100	an indispensable enzyme for polyglucosan formation	1078:1127	Nonetheless, we observe that CA contains ubiquitin and p62, both of them associated with processes of elimination of waste substances, and also glycogen synthase, an indispensable enzyme for polyglucosan formation.
30202002	3	10	theme	antibodies	437:446	arg1	existence					407:415	the existence	403:415	the existence of some natural IgM antibodies directed against these neo-epitopes	403:482	In previous work, we revealed the presence of some neo-epitopes in CA and the existence of some natural IgM antibodies directed against these neo-epitopes.
30202002	3	10	theme	antibodies	437:446	arg1	presence					363:370	the presence	359:370	the presence of some neo-epitopes in CA	359:397	In previous work, we revealed the presence of some neo-epitopes in CA and the existence of some natural IgM antibodies directed against these neo-epitopes.
30202002	4	11	from	cause	541:545	arg1	studies					591:597	CA immunohistochemical studies	568:597	CA immunohistochemical studies	568:597	We also noted that these neo-epitopes and IgMs were the cause of false staining in CA immunohistochemical studies, and disproved the proposed presence of β-amyloid peptides and tau protein in them.
30202002	4	12	from	presence	627:634	arg1	them					677:680	them	677:680	them	677:680	We also noted that these neo-epitopes and IgMs were the cause of false staining in CA immunohistochemical studies, and disproved the proposed presence of β-amyloid peptides and tau protein in them.
30202002	4	13	theme	proposed	618:625	arg1	presence					627:634	the proposed presence	614:634	the proposed presence of β-amyloid peptides and tau protein in them	614:680	We also noted that these neo-epitopes and IgMs were the cause of false staining in CA immunohistochemical studies, and disproved the proposed presence of β-amyloid peptides and tau protein in them.
30202002	7	14	theme	waste	1032:1036	arg1	substances					1038:1047	waste substances	1032:1047	waste substances	1032:1047	Nonetheless, we observe that CA contains ubiquitin and p62, both of them associated with processes of elimination of waste substances, and also glycogen synthase, an indispensable enzyme for polyglucosan formation.
30202002	1	15	theme	Corpora	70:76	arg1	bodies					109:114	polyglucosan bodies	96:114	polyglucosan bodies that accumulate in the human brain during ageing and are also present in large numbers in neurodegenerative conditions	96:233	Corpora amylacea (CA) are polyglucosan bodies that accumulate in the human brain during ageing and are also present in large numbers in neurodegenerative conditions.
30202002	1	15	theme	Corpora	70:76	arg1	CA					88:89	CA	88:89	CA	88:89	Corpora amylacea (CA) are polyglucosan bodies that accumulate in the human brain during ageing and are also present in large numbers in neurodegenerative conditions.
30202002	1	15	theme	Corpora	70:76	arg1	amylacea					78:85	Corpora amylacea	70:85	Corpora amylacea (CA)	70:90	Corpora amylacea (CA) are polyglucosan bodies that accumulate in the human brain during ageing and are also present in large numbers in neurodegenerative conditions.
30202002	4	16	dep	neo-epitopes	510:521	arg1	these					504:508	these	504:508	these	504:508	We also noted that these neo-epitopes and IgMs were the cause of false staining in CA immunohistochemical studies, and disproved the proposed presence of β-amyloid peptides and tau protein in them.
30202002	7	17	theme	polyglucosan	1106:1117	arg1	formation					1119:1127	polyglucosan formation	1106:1127	polyglucosan formation	1106:1127	Nonetheless, we observe that CA contains ubiquitin and p62, both of them associated with processes of elimination of waste substances, and also glycogen synthase, an indispensable enzyme for polyglucosan formation.
30202002	7	18	theme	glycogen	1059:1066	arg1	synthase					1068:1075	glycogen synthase	1059:1075	glycogen synthase	1059:1075	Nonetheless, we observe that CA contains ubiquitin and p62, both of them associated with processes of elimination of waste substances, and also glycogen synthase, an indispensable enzyme for polyglucosan formation.
30202002	7	18	theme	glycogen	1059:1066	arg1	enzyme					1095:1100	an indispensable enzyme	1078:1100	an indispensable enzyme for polyglucosan formation	1078:1127	Nonetheless, we observe that CA contains ubiquitin and p62, both of them associated with processes of elimination of waste substances, and also glycogen synthase, an indispensable enzyme for polyglucosan formation.
30202002	1	19	attach	present	178:184	arg2	amylacea					78:85	Corpora amylacea	70:85	Corpora amylacea (CA)	70:90	Corpora amylacea (CA) are polyglucosan bodies that accumulate in the human brain during ageing and are also present in large numbers in neurodegenerative conditions.
30202002	1	19	attach	present	178:184	arg1	numbers					195:201	large numbers	189:201	large numbers in neurodegenerative conditions	189:233	Corpora amylacea (CA) are polyglucosan bodies that accumulate in the human brain during ageing and are also present in large numbers in neurodegenerative conditions.
30202002	1	19	attach	present	178:184	arg2	bodies					109:114	polyglucosan bodies	96:114	polyglucosan bodies that accumulate in the human brain during ageing and are also present in large numbers in neurodegenerative conditions	96:233	Corpora amylacea (CA) are polyglucosan bodies that accumulate in the human brain during ageing and are also present in large numbers in neurodegenerative conditions.
30202002	7	20	contain	contains	947:954	arg2	ubiquitin					956:964	ubiquitin	956:964	ubiquitin	956:964	Nonetheless, we observe that CA contains ubiquitin and p62, both of them associated with processes of elimination of waste substances, and also glycogen synthase, an indispensable enzyme for polyglucosan formation.
30202002	7	20	contain	contains	947:954	arg2	them					983:986	them	983:986	them	983:986	Nonetheless, we observe that CA contains ubiquitin and p62, both of them associated with processes of elimination of waste substances, and also glycogen synthase, an indispensable enzyme for polyglucosan formation.
30202002	7	20	contain	contains	947:954	arg2	p62					970:972	p62	970:972	p62	970:972	Nonetheless, we observe that CA contains ubiquitin and p62, both of them associated with processes of elimination of waste substances, and also glycogen synthase, an indispensable enzyme for polyglucosan formation.
30202002	7	20	contain	contains	947:954	arg1	CA					944:945	CA	944:945	CA	944:945	Nonetheless, we observe that CA contains ubiquitin and p62, both of them associated with processes of elimination of waste substances, and also glycogen synthase, an indispensable enzyme for polyglucosan formation.
30202002	7	20	contain	contains	947:954	arg2	synthase					1068:1075	glycogen synthase	1059:1075	glycogen synthase	1059:1075	Nonetheless, we observe that CA contains ubiquitin and p62, both of them associated with processes of elimination of waste substances, and also glycogen synthase, an indispensable enzyme for polyglucosan formation.
30202002	7	20	contain	contains	947:954	arg2	enzyme					1095:1100	an indispensable enzyme	1078:1100	an indispensable enzyme for polyglucosan formation	1078:1127	Nonetheless, we observe that CA contains ubiquitin and p62, both of them associated with processes of elimination of waste substances, and also glycogen synthase, an indispensable enzyme for polyglucosan formation.
30202002	7	20	contain	contains	947:954	arg2	both					975:978	both	975:978	both	975:978	Nonetheless, we observe that CA contains ubiquitin and p62, both of them associated with processes of elimination of waste substances, and also glycogen synthase, an indispensable enzyme for polyglucosan formation.
30202002	6	21	contain	contain	810:816	arg2	S100					824:827	S100	824:827	S100	824:827	We show that, contrary to previous descriptions, CA do not contain GFAP, S100, AQP4, NeuN or class III β-tubulin, and we question the presence of other components.
30202002	6	21	contain	contain	810:816	arg2	AQP4					830:833	AQP4	830:833	AQP4	830:833	We show that, contrary to previous descriptions, CA do not contain GFAP, S100, AQP4, NeuN or class III β-tubulin, and we question the presence of other components.
30202002	6	21	contain	contain	810:816	arg2	β-tubulin					854:862	class III β-tubulin	844:862	class III β-tubulin	844:862	We show that, contrary to previous descriptions, CA do not contain GFAP, S100, AQP4, NeuN or class III β-tubulin, and we question the presence of other components.
30202002	6	21	contain	contain	810:816	arg2	NeuN					836:839	NeuN	836:839	NeuN	836:839	We show that, contrary to previous descriptions, CA do not contain GFAP, S100, AQP4, NeuN or class III β-tubulin, and we question the presence of other components.
30202002	6	21	contain	contain	810:816	arg1	CA					800:801	CA	800:801	CA	800:801	We show that, contrary to previous descriptions, CA do not contain GFAP, S100, AQP4, NeuN or class III β-tubulin, and we question the presence of other components.
30202002	6	21	contain	contain	810:816	arg2	GFAP					818:821	GFAP	818:821	GFAP	818:821	We show that, contrary to previous descriptions, CA do not contain GFAP, S100, AQP4, NeuN or class III β-tubulin, and we question the presence of other components.
30202002	0	22	theme	elusive	14:20	arg1	composition					22:32	the elusive composition	10:32	the elusive composition of corpora amylacea of human brain	10:67	Exploring the elusive composition of corpora amylacea of human brain.
30202002	8	23	theme	previous	1203:1210	arg1	studies					1212:1218	previous studies	1203:1218	previous studies about CA	1203:1227	In summary, this study shows that it is imperative to continue reviewing previous studies about CA but, more importantly, it shows that the vision of CA as structures involved in protective or cleaning mechanisms remains the most consistent theory.
30202002	3	24	theme	natural	425:431	arg1	antibodies					437:446	some natural IgM antibodies	420:446	some natural IgM antibodies directed against these neo-epitopes	420:482	In previous work, we revealed the presence of some neo-epitopes in CA and the existence of some natural IgM antibodies directed against these neo-epitopes.
30202002	0	25	theme	corpora	37:43	arg1	amylacea					45:52	corpora amylacea	37:52	corpora amylacea of human brain	37:67	Exploring the elusive composition of corpora amylacea of human brain.
30202002	4	26	theme	protein	666:672	arg1	presence					627:634	the proposed presence	614:634	the proposed presence of β-amyloid peptides and tau protein in them	614:680	We also noted that these neo-epitopes and IgMs were the cause of false staining in CA immunohistochemical studies, and disproved the proposed presence of β-amyloid peptides and tau protein in them.
30202002	4	27	from	studies	591:597	arg1	IgMs					527:530	IgMs	527:530	IgMs	527:530	We also noted that these neo-epitopes and IgMs were the cause of false staining in CA immunohistochemical studies, and disproved the proposed presence of β-amyloid peptides and tau protein in them.
30202002	4	27	from	studies	591:597	arg1	cause					541:545	the cause	537:545	the cause of false staining in CA immunohistochemical studies	537:597	We also noted that these neo-epitopes and IgMs were the cause of false staining in CA immunohistochemical studies, and disproved the proposed presence of β-amyloid peptides and tau protein in them.
30202002	4	27	from	studies	591:597	arg1	neo-epitopes					510:521	neo-epitopes	510:521	neo-epitopes	510:521	We also noted that these neo-epitopes and IgMs were the cause of false staining in CA immunohistochemical studies, and disproved the proposed presence of β-amyloid peptides and tau protein in them.
30202002	3	28	theme	neo-epitopes	380:391	arg1	existence					407:415	the existence	403:415	the existence of some natural IgM antibodies directed against these neo-epitopes	403:482	In previous work, we revealed the presence of some neo-epitopes in CA and the existence of some natural IgM antibodies directed against these neo-epitopes.
30202002	3	28	theme	neo-epitopes	380:391	arg1	presence					363:370	the presence	359:370	the presence of some neo-epitopes in CA	359:397	In previous work, we revealed the presence of some neo-epitopes in CA and the existence of some natural IgM antibodies directed against these neo-epitopes.
30202002	7	29	theme	substances	1038:1047	arg1	elimination					1017:1027	elimination	1017:1027	elimination of waste substances	1017:1047	Nonetheless, we observe that CA contains ubiquitin and p62, both of them associated with processes of elimination of waste substances, and also glycogen synthase, an indispensable enzyme for polyglucosan formation.
30202002	4	30	attach	presence	627:634	arg1	them					677:680	them	677:680	them	677:680	We also noted that these neo-epitopes and IgMs were the cause of false staining in CA immunohistochemical studies, and disproved the proposed presence of β-amyloid peptides and tau protein in them.
30202002	4	30	attach	presence	627:634	arg2	protein					666:672	tau protein	662:672	tau protein	662:672	We also noted that these neo-epitopes and IgMs were the cause of false staining in CA immunohistochemical studies, and disproved the proposed presence of β-amyloid peptides and tau protein in them.
30202002	4	30	attach	presence	627:634	arg2	peptides					649:656	β-amyloid peptides	639:656	β-amyloid peptides	639:656	We also noted that these neo-epitopes and IgMs were the cause of false staining in CA immunohistochemical studies, and disproved the proposed presence of β-amyloid peptides and tau protein in them.
30202002	6	31	theme	components	903:912	arg1	presence					885:892	the presence	881:892	the presence of other components	881:912	We show that, contrary to previous descriptions, CA do not contain GFAP, S100, AQP4, NeuN or class III β-tubulin, and we question the presence of other components.
30202002	1	32	from	present	178:184	arg1	numbers					195:201	large numbers	189:201	large numbers in neurodegenerative conditions	189:233	Corpora amylacea (CA) are polyglucosan bodies that accumulate in the human brain during ageing and are also present in large numbers in neurodegenerative conditions.
30202002	7	33	theme	elimination	1017:1027	arg1	processes					1004:1012	processes	1004:1012	processes of elimination of waste substances	1004:1047	Nonetheless, we observe that CA contains ubiquitin and p62, both of them associated with processes of elimination of waste substances, and also glycogen synthase, an indispensable enzyme for polyglucosan formation.
30202002	8	34	theme	protective	1309:1318	arg1	mechanisms					1332:1341	protective or cleaning mechanisms	1309:1341	protective or cleaning mechanisms	1309:1341	In summary, this study shows that it is imperative to continue reviewing previous studies about CA but, more importantly, it shows that the vision of CA as structures involved in protective or cleaning mechanisms remains the most consistent theory.
30202002	0	35	theme	amylacea	45:52	arg1	composition					22:32	the elusive composition	10:32	the elusive composition of corpora amylacea of human brain	10:67	Exploring the elusive composition of corpora amylacea of human brain.
30202002	6	36	theme	class	844:848	arg1	β-tubulin					854:862	class III β-tubulin	844:862	class III β-tubulin	844:862	We show that, contrary to previous descriptions, CA do not contain GFAP, S100, AQP4, NeuN or class III β-tubulin, and we question the presence of other components.
30202002	8	37	theme	consistent	1360:1369	arg1	theory					1371:1376	the most consistent theory	1351:1376	the most consistent theory	1351:1376	In summary, this study shows that it is imperative to continue reviewing previous studies about CA but, more importantly, it shows that the vision of CA as structures involved in protective or cleaning mechanisms remains the most consistent theory.
30202002	8	38	theme	cleaning	1323:1330	arg1	mechanisms					1332:1341	protective or cleaning mechanisms	1309:1341	protective or cleaning mechanisms	1309:1341	In summary, this study shows that it is imperative to continue reviewing previous studies about CA but, more importantly, it shows that the vision of CA as structures involved in protective or cleaning mechanisms remains the most consistent theory.
30202002	1	39	theme	polyglucosan	96:107	arg1	bodies					109:114	polyglucosan bodies	96:114	polyglucosan bodies that accumulate in the human brain during ageing and are also present in large numbers in neurodegenerative conditions	96:233	Corpora amylacea (CA) are polyglucosan bodies that accumulate in the human brain during ageing and are also present in large numbers in neurodegenerative conditions.
30202002	1	39	theme	polyglucosan	96:107	arg1	amylacea					78:85	Corpora amylacea	70:85	Corpora amylacea (CA)	70:90	Corpora amylacea (CA) are polyglucosan bodies that accumulate in the human brain during ageing and are also present in large numbers in neurodegenerative conditions.
30202002	6	40	theme	previous	777:784	arg1	descriptions					786:797	previous descriptions	777:797	previous descriptions	777:797	We show that, contrary to previous descriptions, CA do not contain GFAP, S100, AQP4, NeuN or class III β-tubulin, and we question the presence of other components.
30202002	8	41	theme	CA	1280:1281	arg1	vision					1270:1275	the vision	1266:1275	the vision of CA as structures involved in protective or cleaning mechanisms	1266:1341	In summary, this study shows that it is imperative to continue reviewing previous studies about CA but, more importantly, it shows that the vision of CA as structures involved in protective or cleaning mechanisms remains the most consistent theory.
30202002	3	42	theme	IgM	433:435	arg1	antibodies					437:446	some natural IgM antibodies	420:446	some natural IgM antibodies directed against these neo-epitopes	420:482	In previous work, we revealed the presence of some neo-epitopes in CA and the existence of some natural IgM antibodies directed against these neo-epitopes.
30202002	1	43	theme	large	189:193	arg1	numbers					195:201	large numbers	189:201	large numbers in neurodegenerative conditions	189:233	Corpora amylacea (CA) are polyglucosan bodies that accumulate in the human brain during ageing and are also present in large numbers in neurodegenerative conditions.
30202002	1	44	from	numbers	195:201	arg1	present					178:184	present	178:184	present	178:184	Corpora amylacea (CA) are polyglucosan bodies that accumulate in the human brain during ageing and are also present in large numbers in neurodegenerative conditions.
30202002	1	44	from	numbers	195:201	arg1	conditions					224:233	neurodegenerative conditions	206:233	neurodegenerative conditions	206:233	Corpora amylacea (CA) are polyglucosan bodies that accumulate in the human brain during ageing and are also present in large numbers in neurodegenerative conditions.
30202002	3	45	from	existence	407:415	arg1	CA					396:397	CA	396:397	CA	396:397	In previous work, we revealed the presence of some neo-epitopes in CA and the existence of some natural IgM antibodies directed against these neo-epitopes.
30202002	2	46	theme	new	299:301	arg1	components					303:312	new components	299:312	new components	299:312	Theories regarding the function of CA are regularly updated as new components are described.
30202002	4	47	theme	staining	556:563	arg1	IgMs					527:530	IgMs	527:530	IgMs	527:530	We also noted that these neo-epitopes and IgMs were the cause of false staining in CA immunohistochemical studies, and disproved the proposed presence of β-amyloid peptides and tau protein in them.
30202002	4	47	theme	staining	556:563	arg1	cause					541:545	the cause	537:545	the cause of false staining in CA immunohistochemical studies	537:597	We also noted that these neo-epitopes and IgMs were the cause of false staining in CA immunohistochemical studies, and disproved the proposed presence of β-amyloid peptides and tau protein in them.
30202002	4	47	theme	staining	556:563	arg1	neo-epitopes					510:521	neo-epitopes	510:521	neo-epitopes	510:521	We also noted that these neo-epitopes and IgMs were the cause of false staining in CA immunohistochemical studies, and disproved the proposed presence of β-amyloid peptides and tau protein in them.
30202002	0	48	theme	brain	63:67	arg1	amylacea					45:52	corpora amylacea	37:52	corpora amylacea of human brain	37:67	Exploring the elusive composition of corpora amylacea of human brain.
30202002	1	49	located	present	178:184	arg2	amylacea					78:85	Corpora amylacea	70:85	Corpora amylacea (CA)	70:90	Corpora amylacea (CA) are polyglucosan bodies that accumulate in the human brain during ageing and are also present in large numbers in neurodegenerative conditions.
30202002	1	49	located	present	178:184	arg1	numbers					195:201	large numbers	189:201	large numbers in neurodegenerative conditions	189:233	Corpora amylacea (CA) are polyglucosan bodies that accumulate in the human brain during ageing and are also present in large numbers in neurodegenerative conditions.
30202002	1	49	located	present	178:184	arg2	bodies					109:114	polyglucosan bodies	96:114	polyglucosan bodies that accumulate in the human brain during ageing and are also present in large numbers in neurodegenerative conditions	96:233	Corpora amylacea (CA) are polyglucosan bodies that accumulate in the human brain during ageing and are also present in large numbers in neurodegenerative conditions.
30202002	3	50	from	CA	396:397	arg1	existence					407:415	the existence	403:415	the existence of some natural IgM antibodies directed against these neo-epitopes	403:482	In previous work, we revealed the presence of some neo-epitopes in CA and the existence of some natural IgM antibodies directed against these neo-epitopes.
30202002	3	50	from	CA	396:397	arg1	presence					363:370	the presence	359:370	the presence of some neo-epitopes in CA	359:397	In previous work, we revealed the presence of some neo-epitopes in CA and the existence of some natural IgM antibodies directed against these neo-epitopes.
30202002	4	51	theme	peptides	649:656	arg1	presence					627:634	the proposed presence	614:634	the proposed presence of β-amyloid peptides and tau protein in them	614:680	We also noted that these neo-epitopes and IgMs were the cause of false staining in CA immunohistochemical studies, and disproved the proposed presence of β-amyloid peptides and tau protein in them.
30202002	4	52	theme	immunohistochemical	571:589	arg1	studies					591:597	CA immunohistochemical studies	568:597	CA immunohistochemical studies	568:597	We also noted that these neo-epitopes and IgMs were the cause of false staining in CA immunohistochemical studies, and disproved the proposed presence of β-amyloid peptides and tau protein in them.
30202002	0	53	theme	human	57:61	arg1	brain					63:67	human brain	57:67	human brain	57:67	Exploring the elusive composition of corpora amylacea of human brain.
30202002	3	54	from	presence	363:370	arg1	CA					396:397	CA	396:397	CA	396:397	In previous work, we revealed the presence of some neo-epitopes in CA and the existence of some natural IgM antibodies directed against these neo-epitopes.
30202002	4	55	theme	CA	568:569	arg1	studies					591:597	CA immunohistochemical studies	568:597	CA immunohistochemical studies	568:597	We also noted that these neo-epitopes and IgMs were the cause of false staining in CA immunohistochemical studies, and disproved the proposed presence of β-amyloid peptides and tau protein in them.
30202002	4	56	theme	β-amyloid	639:647	arg1	peptides					649:656	β-amyloid peptides	639:656	β-amyloid peptides	639:656	We also noted that these neo-epitopes and IgMs were the cause of false staining in CA immunohistochemical studies, and disproved the proposed presence of β-amyloid peptides and tau protein in them.
30202002	1	57	theme	neurodegenerative	206:222	arg1	conditions					224:233	neurodegenerative conditions	206:233	neurodegenerative conditions	206:233	Corpora amylacea (CA) are polyglucosan bodies that accumulate in the human brain during ageing and are also present in large numbers in neurodegenerative conditions.
29958771	3	0	theme	maximum	668:674	arg1	productivity					676:687	a maximum productivity	666:687	a maximum productivity of 6.01 mg L-1 d-1	666:706	We found that SN conditions maximized fucoxanthin accumulation: a maximum productivity of 6.01 mg L-1 d-1 was obtained, a 4.32-fold and 1.42-fold increase over production in the ILN and IHN groups, respectively.
29958771	2	1	theme	eicosapentaenoic	573:588	arg1	components					528:537	three high-value bioactive components	501:537	three high-value bioactive components: fucoxanthin, chrysolaminarin and eicosapentaenoic acid (EPA)	501:599	We subjected the marine diatom Odontella aurita to three nitrogen supply regimes [initial low nitrogen (ILN), initial high nitrogen (IHN), and initial high nitrogen plus supplementary nitrogen (SN)] to investigate the accumulation of three high-value bioactive components: fucoxanthin, chrysolaminarin and eicosapentaenoic acid (EPA).
29958771	2	1	theme	eicosapentaenoic	573:588	arg1	EPA					596:598	EPA	596:598	EPA	596:598	We subjected the marine diatom Odontella aurita to three nitrogen supply regimes [initial low nitrogen (ILN), initial high nitrogen (IHN), and initial high nitrogen plus supplementary nitrogen (SN)] to investigate the accumulation of three high-value bioactive components: fucoxanthin, chrysolaminarin and eicosapentaenoic acid (EPA).
29958771	2	1	theme	eicosapentaenoic	573:588	arg1	acid					590:593	eicosapentaenoic acid	573:593	eicosapentaenoic acid (EPA)	573:599	We subjected the marine diatom Odontella aurita to three nitrogen supply regimes [initial low nitrogen (ILN), initial high nitrogen (IHN), and initial high nitrogen plus supplementary nitrogen (SN)] to investigate the accumulation of three high-value bioactive components: fucoxanthin, chrysolaminarin and eicosapentaenoic acid (EPA).
29958771	1	2	theme	promising	160:168	arg1	Microalgae					131:140	Microalgae	131:140	Microalgae	131:140	Microalgae are recognized as promising producers of many bioactive products, but their utility is limited due to high production costs.
29958771	1	2	theme	promising	160:168	arg1	producers					170:178	promising producers	160:178	promising producers of many bioactive products	160:205	Microalgae are recognized as promising producers of many bioactive products, but their utility is limited due to high production costs.
29958771	5	3	theme	DW	1015:1016	arg1	DW					979:980	DW	979:980	DW	979:980	Chrysolaminarin content rose to 60.33% of dry weight (DW) in the ILN group, and 46.27% of DW in the IHN group.
29958771	5	3	theme	DW	1015:1016	arg1	%					962:962	60.33%	957:962	60.33% of dry weight (DW) in the ILN group	957:998	Chrysolaminarin content rose to 60.33% of dry weight (DW) in the ILN group, and 46.27% of DW in the IHN group.
29958771	5	3	theme	DW	1015:1016	arg1	weight					971:976	dry weight	967:976	dry weight (DW) in the ILN group	967:998	Chrysolaminarin content rose to 60.33% of dry weight (DW) in the ILN group, and 46.27% of DW in the IHN group.
29958771	5	3	theme	DW	1015:1016	arg1	%					1010:1010	46.27%	1005:1010	46.27% of DW	1005:1016	Chrysolaminarin content rose to 60.33% of dry weight (DW) in the ILN group, and 46.27% of DW in the IHN group.
29958771	5	3	theme	DW	1015:1016	arg1	DW					1015:1016	DW	1015:1016	DW	1015:1016	Chrysolaminarin content rose to 60.33% of dry weight (DW) in the ILN group, and 46.27% of DW in the IHN group.
29958771	1	4	theme	high	244:247	arg1	costs					260:264	high production costs	244:264	high production costs	244:264	Microalgae are recognized as promising producers of many bioactive products, but their utility is limited due to high production costs.
29958771	8	5	theme	compound	1415:1422	arg1	content					1397:1403	overall content	1389:1403	overall content of either compound	1389:1422	However, IHN conditions did not maximize overall content of either compound.
29958771	2	6	theme	[initial	348:355	arg1	ILN					371:373	ILN	371:373	ILN	371:373	We subjected the marine diatom Odontella aurita to three nitrogen supply regimes [initial low nitrogen (ILN), initial high nitrogen (IHN), and initial high nitrogen plus supplementary nitrogen (SN)] to investigate the accumulation of three high-value bioactive components: fucoxanthin, chrysolaminarin and eicosapentaenoic acid (EPA).
29958771	2	6	theme	[initial	348:355	arg1	nitrogen					361:368	[initial low nitrogen	348:368	three nitrogen supply regimes [initial low nitrogen (ILN)	318:374	We subjected the marine diatom Odontella aurita to three nitrogen supply regimes [initial low nitrogen (ILN), initial high nitrogen (IHN), and initial high nitrogen plus supplementary nitrogen (SN)] to investigate the accumulation of three high-value bioactive components: fucoxanthin, chrysolaminarin and eicosapentaenoic acid (EPA).
29958771	1	7	theme	production	249:258	arg1	costs					260:264	high production costs	244:264	high production costs	244:264	Microalgae are recognized as promising producers of many bioactive products, but their utility is limited due to high production costs.
29958771	0	8	theme	different	96:104	arg1	regimes					122:128	different nitrogen supply regimes	96:128	different nitrogen supply regimes	96:128	Production of fucoxanthin, chrysolaminarin, and eicosapentaenoic acid by Odontella aurita under different nitrogen supply regimes.
29958771	3	9	from	increase	748:755	arg1	groups					792:797	the ILN and IHN groups	776:797	the ILN and IHN groups	776:797	We found that SN conditions maximized fucoxanthin accumulation: a maximum productivity of 6.01 mg L-1 d-1 was obtained, a 4.32-fold and 1.42-fold increase over production in the ILN and IHN groups, respectively.
29958771	9	10	theme	products	1527:1534	arg1	products					1527:1534	bioactive products	1517:1534	bioactive products	1517:1534	Our results demonstrated that O. aurita is potentially useful as a producer of a variety of bioactive products; the compounds produced by this species can be controlled by altering the nitrogen supply.
29958771	9	10	theme	products	1527:1534	arg1	variety					1506:1512	a variety	1504:1512	a variety of bioactive products	1504:1534	Our results demonstrated that O. aurita is potentially useful as a producer of a variety of bioactive products; the compounds produced by this species can be controlled by altering the nitrogen supply.
29958771	2	11	theme	marine	284:289	arg1	aurita					308:313	the marine diatom Odontella aurita	280:313	the marine diatom Odontella aurita	280:313	We subjected the marine diatom Odontella aurita to three nitrogen supply regimes [initial low nitrogen (ILN), initial high nitrogen (IHN), and initial high nitrogen plus supplementary nitrogen (SN)] to investigate the accumulation of three high-value bioactive components: fucoxanthin, chrysolaminarin and eicosapentaenoic acid (EPA).
29958771	5	12	theme	Chrysolaminarin	925:939	arg1	content					941:947	Chrysolaminarin content	925:947	Chrysolaminarin content	925:947	Chrysolaminarin content rose to 60.33% of dry weight (DW) in the ILN group, and 46.27% of DW in the IHN group.
29958771	4	13	theme	storage	907:913	arg1	compound					915:922	the dominant energy storage compound	887:922	the dominant energy storage compound	887:922	After nitrogen was depleted in the growth medium, chrysolaminarin became the dominant energy storage compound.
29958771	3	14	theme	6.01 mg L-1 d-1	692:706	arg1	productivity					676:687	a maximum productivity	666:687	a maximum productivity of 6.01 mg L-1 d-1	666:706	We found that SN conditions maximized fucoxanthin accumulation: a maximum productivity of 6.01 mg L-1 d-1 was obtained, a 4.32-fold and 1.42-fold increase over production in the ILN and IHN groups, respectively.
29958771	6	15	theme	nitrogen	1195:1202	arg1	supply					1204:1209	nitrogen supply	1195:1209	nitrogen supply	1195:1209	Variations in fatty acid composition across the different nitrogen supply regimes indicated that EPA primarily accumulated in the glycolipids, especially when nitrogen supply was sufficient.
29958771	2	16	theme	low	357:359	arg1	ILN					371:373	ILN	371:373	ILN	371:373	We subjected the marine diatom Odontella aurita to three nitrogen supply regimes [initial low nitrogen (ILN), initial high nitrogen (IHN), and initial high nitrogen plus supplementary nitrogen (SN)] to investigate the accumulation of three high-value bioactive components: fucoxanthin, chrysolaminarin and eicosapentaenoic acid (EPA).
29958771	2	16	theme	low	357:359	arg1	nitrogen					361:368	[initial low nitrogen	348:368	three nitrogen supply regimes [initial low nitrogen (ILN)	318:374	We subjected the marine diatom Odontella aurita to three nitrogen supply regimes [initial low nitrogen (ILN), initial high nitrogen (IHN), and initial high nitrogen plus supplementary nitrogen (SN)] to investigate the accumulation of three high-value bioactive components: fucoxanthin, chrysolaminarin and eicosapentaenoic acid (EPA).
29958771	3	17	theme	IHN	788:790	arg1	groups					792:797	the ILN and IHN groups	776:797	the ILN and IHN groups	776:797	We found that SN conditions maximized fucoxanthin accumulation: a maximum productivity of 6.01 mg L-1 d-1 was obtained, a 4.32-fold and 1.42-fold increase over production in the ILN and IHN groups, respectively.
29958771	0	18	theme	supply	115:120	arg1	regimes					122:128	different nitrogen supply regimes	96:128	different nitrogen supply regimes	96:128	Production of fucoxanthin, chrysolaminarin, and eicosapentaenoic acid by Odontella aurita under different nitrogen supply regimes.
29958771	6	19	theme	different	1084:1092	arg1	regimes					1110:1116	the different nitrogen supply regimes	1080:1116	the different nitrogen supply regimes	1080:1116	Variations in fatty acid composition across the different nitrogen supply regimes indicated that EPA primarily accumulated in the glycolipids, especially when nitrogen supply was sufficient.
29958771	5	20	from	weight	971:976	arg1	group					994:998	the ILN group	986:998	the ILN group	986:998	Chrysolaminarin content rose to 60.33% of dry weight (DW) in the ILN group, and 46.27% of DW in the IHN group.
29958771	2	21	theme	initial	377:383	arg1	nitrogen					390:397	initial high nitrogen	377:397	initial high nitrogen (IHN)	377:403	We subjected the marine diatom Odontella aurita to three nitrogen supply regimes [initial low nitrogen (ILN), initial high nitrogen (IHN), and initial high nitrogen plus supplementary nitrogen (SN)] to investigate the accumulation of three high-value bioactive components: fucoxanthin, chrysolaminarin and eicosapentaenoic acid (EPA).
29958771	2	21	theme	initial	377:383	arg1	IHN					400:402	IHN	400:402	IHN	400:402	We subjected the marine diatom Odontella aurita to three nitrogen supply regimes [initial low nitrogen (ILN), initial high nitrogen (IHN), and initial high nitrogen plus supplementary nitrogen (SN)] to investigate the accumulation of three high-value bioactive components: fucoxanthin, chrysolaminarin and eicosapentaenoic acid (EPA).
29958771	0	22	theme	nitrogen	106:113	arg1	regimes					122:128	different nitrogen supply regimes	96:128	different nitrogen supply regimes	96:128	Production of fucoxanthin, chrysolaminarin, and eicosapentaenoic acid by Odontella aurita under different nitrogen supply regimes.
29958771	7	23	located	observed	1321:1328	arg1	group					1341:1345	the IHN group	1333:1345	the IHN group	1333:1345	The maximum productivity of chrysolaminarin (161.55 mg L-1 d-1) and EPA (9.37 mg L-1 d-1) was observed in the IHN group.
29958771	7	23	located	observed	1321:1328	arg2	productivity					1239:1250	The maximum productivity	1227:1250	The maximum productivity of chrysolaminarin (161.55 mg L-1 d-1) and EPA (9.37 mg L-1 d-1)	1227:1315	The maximum productivity of chrysolaminarin (161.55 mg L-1 d-1) and EPA (9.37 mg L-1 d-1) was observed in the IHN group.
29958771	6	24	theme	acid	1056:1059	arg1	composition					1061:1071	fatty acid composition	1050:1071	fatty acid composition across the different nitrogen supply regimes	1050:1116	Variations in fatty acid composition across the different nitrogen supply regimes indicated that EPA primarily accumulated in the glycolipids, especially when nitrogen supply was sufficient.
29958771	5	25	from	%	962:962	arg1	group					994:998	the ILN group	986:998	the ILN group	986:998	Chrysolaminarin content rose to 60.33% of dry weight (DW) in the ILN group, and 46.27% of DW in the IHN group.
29958771	1	26	theme	many	183:186	arg1	products					198:205	many bioactive products	183:205	many bioactive products	183:205	Microalgae are recognized as promising producers of many bioactive products, but their utility is limited due to high production costs.
29958771	2	27	theme	initial	410:416	arg1	SN					461:462	SN	461:462	SN	461:462	We subjected the marine diatom Odontella aurita to three nitrogen supply regimes [initial low nitrogen (ILN), initial high nitrogen (IHN), and initial high nitrogen plus supplementary nitrogen (SN)] to investigate the accumulation of three high-value bioactive components: fucoxanthin, chrysolaminarin and eicosapentaenoic acid (EPA).
29958771	2	27	theme	initial	410:416	arg1	nitrogen					423:430	initial high nitrogen	410:430	initial high nitrogen	410:430	We subjected the marine diatom Odontella aurita to three nitrogen supply regimes [initial low nitrogen (ILN), initial high nitrogen (IHN), and initial high nitrogen plus supplementary nitrogen (SN)] to investigate the accumulation of three high-value bioactive components: fucoxanthin, chrysolaminarin and eicosapentaenoic acid (EPA).
29958771	5	28	theme	IHN	1025:1027	arg1	group					1029:1033	the IHN group	1021:1033	the IHN group	1021:1033	Chrysolaminarin content rose to 60.33% of dry weight (DW) in the ILN group, and 46.27% of DW in the IHN group.
29958771	5	29	from	group	994:998	arg1	DW					979:980	DW	979:980	DW	979:980	Chrysolaminarin content rose to 60.33% of dry weight (DW) in the ILN group, and 46.27% of DW in the IHN group.
29958771	5	29	from	group	994:998	arg1	%					962:962	60.33%	957:962	60.33% of dry weight (DW) in the ILN group	957:998	Chrysolaminarin content rose to 60.33% of dry weight (DW) in the ILN group, and 46.27% of DW in the IHN group.
29958771	5	29	from	group	994:998	arg1	weight					971:976	dry weight	967:976	dry weight (DW) in the ILN group	967:998	Chrysolaminarin content rose to 60.33% of dry weight (DW) in the ILN group, and 46.27% of DW in the IHN group.
29958771	5	29	from	group	994:998	arg1	%					1010:1010	46.27%	1005:1010	46.27% of DW	1005:1016	Chrysolaminarin content rose to 60.33% of dry weight (DW) in the ILN group, and 46.27% of DW in the IHN group.
29958771	5	29	from	group	994:998	arg1	DW					1015:1016	DW	1015:1016	DW	1015:1016	Chrysolaminarin content rose to 60.33% of dry weight (DW) in the ILN group, and 46.27% of DW in the IHN group.
29958771	7	30	theme	IHN	1337:1339	arg1	group					1341:1345	the IHN group	1333:1345	the IHN group	1333:1345	The maximum productivity of chrysolaminarin (161.55 mg L-1 d-1) and EPA (9.37 mg L-1 d-1) was observed in the IHN group.
29958771	1	31	theme	bioactive	188:196	arg1	products					198:205	many bioactive products	183:205	many bioactive products	183:205	Microalgae are recognized as promising producers of many bioactive products, but their utility is limited due to high production costs.
29958771	1	32	theme	products	198:205	arg1	Microalgae					131:140	Microalgae	131:140	Microalgae	131:140	Microalgae are recognized as promising producers of many bioactive products, but their utility is limited due to high production costs.
29958771	1	32	theme	products	198:205	arg1	producers					170:178	promising producers	160:178	promising producers of many bioactive products	160:205	Microalgae are recognized as promising producers of many bioactive products, but their utility is limited due to high production costs.
29958771	3	33	theme	4.32-fold	724:732	arg1	increase					748:755	a 4.32-fold and 1.42-fold increase	722:755	a 4.32-fold and 1.42-fold increase over production in the ILN and IHN groups	722:797	We found that SN conditions maximized fucoxanthin accumulation: a maximum productivity of 6.01 mg L-1 d-1 was obtained, a 4.32-fold and 1.42-fold increase over production in the ILN and IHN groups, respectively.
29958771	2	34	theme	high-value	507:516	arg1	components					528:537	three high-value bioactive components	501:537	three high-value bioactive components: fucoxanthin, chrysolaminarin and eicosapentaenoic acid (EPA)	501:599	We subjected the marine diatom Odontella aurita to three nitrogen supply regimes [initial low nitrogen (ILN), initial high nitrogen (IHN), and initial high nitrogen plus supplementary nitrogen (SN)] to investigate the accumulation of three high-value bioactive components: fucoxanthin, chrysolaminarin and eicosapentaenoic acid (EPA).
29958771	2	34	theme	high-value	507:516	arg1	chrysolaminarin					553:567	chrysolaminarin	553:567	chrysolaminarin	553:567	We subjected the marine diatom Odontella aurita to three nitrogen supply regimes [initial low nitrogen (ILN), initial high nitrogen (IHN), and initial high nitrogen plus supplementary nitrogen (SN)] to investigate the accumulation of three high-value bioactive components: fucoxanthin, chrysolaminarin and eicosapentaenoic acid (EPA).
29958771	2	34	theme	high-value	507:516	arg1	acid					590:593	eicosapentaenoic acid	573:593	eicosapentaenoic acid (EPA)	573:599	We subjected the marine diatom Odontella aurita to three nitrogen supply regimes [initial low nitrogen (ILN), initial high nitrogen (IHN), and initial high nitrogen plus supplementary nitrogen (SN)] to investigate the accumulation of three high-value bioactive components: fucoxanthin, chrysolaminarin and eicosapentaenoic acid (EPA).
29958771	2	34	theme	high-value	507:516	arg1	fucoxanthin					540:550	fucoxanthin	540:550	fucoxanthin	540:550	We subjected the marine diatom Odontella aurita to three nitrogen supply regimes [initial low nitrogen (ILN), initial high nitrogen (IHN), and initial high nitrogen plus supplementary nitrogen (SN)] to investigate the accumulation of three high-value bioactive components: fucoxanthin, chrysolaminarin and eicosapentaenoic acid (EPA).
29958771	2	35	theme	Odontella	298:306	arg1	aurita					308:313	the marine diatom Odontella aurita	280:313	the marine diatom Odontella aurita	280:313	We subjected the marine diatom Odontella aurita to three nitrogen supply regimes [initial low nitrogen (ILN), initial high nitrogen (IHN), and initial high nitrogen plus supplementary nitrogen (SN)] to investigate the accumulation of three high-value bioactive components: fucoxanthin, chrysolaminarin and eicosapentaenoic acid (EPA).
29958771	7	36	theme	maximum	1231:1237	arg1	productivity					1239:1250	The maximum productivity	1227:1250	The maximum productivity of chrysolaminarin (161.55 mg L-1 d-1) and EPA (9.37 mg L-1 d-1)	1227:1315	The maximum productivity of chrysolaminarin (161.55 mg L-1 d-1) and EPA (9.37 mg L-1 d-1) was observed in the IHN group.
29958771	0	37	theme	fucoxanthin	14:24	arg1	Production					0:9	Production	0:9	Production of fucoxanthin, chrysolaminarin, and eicosapentaenoic acid by Odontella	0:81	Production of fucoxanthin, chrysolaminarin, and eicosapentaenoic acid by Odontella aurita under different nitrogen supply regimes.
29958771	2	38	theme	diatom	291:296	arg1	aurita					308:313	the marine diatom Odontella aurita	280:313	the marine diatom Odontella aurita	280:313	We subjected the marine diatom Odontella aurita to three nitrogen supply regimes [initial low nitrogen (ILN), initial high nitrogen (IHN), and initial high nitrogen plus supplementary nitrogen (SN)] to investigate the accumulation of three high-value bioactive components: fucoxanthin, chrysolaminarin and eicosapentaenoic acid (EPA).
29958771	8	39	theme	IHN	1357:1359	arg1	conditions					1361:1370	IHN conditions	1357:1370	IHN conditions	1357:1370	However, IHN conditions did not maximize overall content of either compound.
29958771	2	40	theme	supplementary	437:449	arg1	nitrogen					451:458	supplementary nitrogen	437:458	supplementary nitrogen	437:458	We subjected the marine diatom Odontella aurita to three nitrogen supply regimes [initial low nitrogen (ILN), initial high nitrogen (IHN), and initial high nitrogen plus supplementary nitrogen (SN)] to investigate the accumulation of three high-value bioactive components: fucoxanthin, chrysolaminarin and eicosapentaenoic acid (EPA).
29958771	4	41	theme	energy	900:905	arg1	storage					907:913	the dominant energy storage	887:913	the dominant energy storage compound	887:922	After nitrogen was depleted in the growth medium, chrysolaminarin became the dominant energy storage compound.
29958771	7	42	theme	chrysolaminarin	1255:1269	arg1	productivity					1239:1250	The maximum productivity	1227:1250	The maximum productivity of chrysolaminarin (161.55 mg L-1 d-1) and EPA (9.37 mg L-1 d-1)	1227:1315	The maximum productivity of chrysolaminarin (161.55 mg L-1 d-1) and EPA (9.37 mg L-1 d-1) was observed in the IHN group.
29958771	0	43	theme	chrysolaminarin	27:41	arg1	Production					0:9	Production	0:9	Production of fucoxanthin, chrysolaminarin, and eicosapentaenoic acid by Odontella	0:81	Production of fucoxanthin, chrysolaminarin, and eicosapentaenoic acid by Odontella aurita under different nitrogen supply regimes.
29958771	4	44	theme	dominant	891:898	arg1	storage					907:913	the dominant energy storage	887:913	the dominant energy storage compound	887:922	After nitrogen was depleted in the growth medium, chrysolaminarin became the dominant energy storage compound.
29958771	9	45	theme	bioactive	1517:1525	arg1	products					1527:1534	bioactive products	1517:1534	bioactive products	1517:1534	Our results demonstrated that O. aurita is potentially useful as a producer of a variety of bioactive products; the compounds produced by this species can be controlled by altering the nitrogen supply.
29958771	2	46	theme	high	385:388	arg1	nitrogen					390:397	initial high nitrogen	377:397	initial high nitrogen (IHN)	377:403	We subjected the marine diatom Odontella aurita to three nitrogen supply regimes [initial low nitrogen (ILN), initial high nitrogen (IHN), and initial high nitrogen plus supplementary nitrogen (SN)] to investigate the accumulation of three high-value bioactive components: fucoxanthin, chrysolaminarin and eicosapentaenoic acid (EPA).
29958771	2	46	theme	high	385:388	arg1	IHN					400:402	IHN	400:402	IHN	400:402	We subjected the marine diatom Odontella aurita to three nitrogen supply regimes [initial low nitrogen (ILN), initial high nitrogen (IHN), and initial high nitrogen plus supplementary nitrogen (SN)] to investigate the accumulation of three high-value bioactive components: fucoxanthin, chrysolaminarin and eicosapentaenoic acid (EPA).
29958771	0	47	theme	eicosapentaenoic	48:63	arg1	acid					65:68	eicosapentaenoic acid	48:68	eicosapentaenoic acid	48:68	Production of fucoxanthin, chrysolaminarin, and eicosapentaenoic acid by Odontella aurita under different nitrogen supply regimes.
29958771	5	48	from	%	1010:1010	arg1	group					994:998	the ILN group	986:998	the ILN group	986:998	Chrysolaminarin content rose to 60.33% of dry weight (DW) in the ILN group, and 46.27% of DW in the IHN group.
29958771	9	49	theme	nitrogen	1610:1617	arg1	supply					1619:1624	the nitrogen supply	1606:1624	the nitrogen supply	1606:1624	Our results demonstrated that O. aurita is potentially useful as a producer of a variety of bioactive products; the compounds produced by this species can be controlled by altering the nitrogen supply.
29958771	2	50	theme	high	418:421	arg1	SN					461:462	SN	461:462	SN	461:462	We subjected the marine diatom Odontella aurita to three nitrogen supply regimes [initial low nitrogen (ILN), initial high nitrogen (IHN), and initial high nitrogen plus supplementary nitrogen (SN)] to investigate the accumulation of three high-value bioactive components: fucoxanthin, chrysolaminarin and eicosapentaenoic acid (EPA).
29958771	2	50	theme	high	418:421	arg1	nitrogen					423:430	initial high nitrogen	410:430	initial high nitrogen	410:430	We subjected the marine diatom Odontella aurita to three nitrogen supply regimes [initial low nitrogen (ILN), initial high nitrogen (IHN), and initial high nitrogen plus supplementary nitrogen (SN)] to investigate the accumulation of three high-value bioactive components: fucoxanthin, chrysolaminarin and eicosapentaenoic acid (EPA).
29958771	6	51	theme	nitrogen	1094:1101	arg1	regimes					1110:1116	the different nitrogen supply regimes	1080:1116	the different nitrogen supply regimes	1080:1116	Variations in fatty acid composition across the different nitrogen supply regimes indicated that EPA primarily accumulated in the glycolipids, especially when nitrogen supply was sufficient.
29958771	4	52	theme	growth	849:854	arg1	medium					856:861	the growth medium	845:861	the growth medium	845:861	After nitrogen was depleted in the growth medium, chrysolaminarin became the dominant energy storage compound.
29958771	5	53	theme	ILN	990:992	arg1	group					994:998	the ILN group	986:998	the ILN group	986:998	Chrysolaminarin content rose to 60.33% of dry weight (DW) in the ILN group, and 46.27% of DW in the IHN group.
29958771	2	54	theme	supply	333:338	arg1	regimes					340:346	three nitrogen supply regimes [initial low nitrogen (ILN)	318:374	three nitrogen supply regimes [initial low nitrogen (ILN)	318:374	We subjected the marine diatom Odontella aurita to three nitrogen supply regimes [initial low nitrogen (ILN), initial high nitrogen (IHN), and initial high nitrogen plus supplementary nitrogen (SN)] to investigate the accumulation of three high-value bioactive components: fucoxanthin, chrysolaminarin and eicosapentaenoic acid (EPA).
29958771	3	55	theme	1.42-fold	738:746	arg1	increase					748:755	a 4.32-fold and 1.42-fold increase	722:755	a 4.32-fold and 1.42-fold increase over production in the ILN and IHN groups	722:797	We found that SN conditions maximized fucoxanthin accumulation: a maximum productivity of 6.01 mg L-1 d-1 was obtained, a 4.32-fold and 1.42-fold increase over production in the ILN and IHN groups, respectively.
29958771	2	56	theme	components	528:537	arg1	accumulation					485:496	the accumulation	481:496	the accumulation of three high-value bioactive components: fucoxanthin, chrysolaminarin and eicosapentaenoic acid (EPA)	481:599	We subjected the marine diatom Odontella aurita to three nitrogen supply regimes [initial low nitrogen (ILN), initial high nitrogen (IHN), and initial high nitrogen plus supplementary nitrogen (SN)] to investigate the accumulation of three high-value bioactive components: fucoxanthin, chrysolaminarin and eicosapentaenoic acid (EPA).
29958771	3	57	theme	fucoxanthin	640:650	arg1	accumulation					652:663	fucoxanthin accumulation	640:663	fucoxanthin accumulation	640:663	We found that SN conditions maximized fucoxanthin accumulation: a maximum productivity of 6.01 mg L-1 d-1 was obtained, a 4.32-fold and 1.42-fold increase over production in the ILN and IHN groups, respectively.
29958771	7	58	theme	EPA	1295:1297	arg1	productivity					1239:1250	The maximum productivity	1227:1250	The maximum productivity of chrysolaminarin (161.55 mg L-1 d-1) and EPA (9.37 mg L-1 d-1)	1227:1315	The maximum productivity of chrysolaminarin (161.55 mg L-1 d-1) and EPA (9.37 mg L-1 d-1) was observed in the IHN group.
29958771	6	59	from	Variations	1036:1045	arg1	composition					1061:1071	fatty acid composition	1050:1071	fatty acid composition across the different nitrogen supply regimes	1050:1116	Variations in fatty acid composition across the different nitrogen supply regimes indicated that EPA primarily accumulated in the glycolipids, especially when nitrogen supply was sufficient.
29958771	2	60	theme	nitrogen	324:331	arg1	regimes					340:346	three nitrogen supply regimes [initial low nitrogen (ILN)	318:374	three nitrogen supply regimes [initial low nitrogen (ILN)	318:374	We subjected the marine diatom Odontella aurita to three nitrogen supply regimes [initial low nitrogen (ILN), initial high nitrogen (IHN), and initial high nitrogen plus supplementary nitrogen (SN)] to investigate the accumulation of three high-value bioactive components: fucoxanthin, chrysolaminarin and eicosapentaenoic acid (EPA).
29958771	2	61	dep	regimes	340:346	arg1	ILN					371:373	ILN	371:373	ILN	371:373	We subjected the marine diatom Odontella aurita to three nitrogen supply regimes [initial low nitrogen (ILN), initial high nitrogen (IHN), and initial high nitrogen plus supplementary nitrogen (SN)] to investigate the accumulation of three high-value bioactive components: fucoxanthin, chrysolaminarin and eicosapentaenoic acid (EPA).
29958771	2	61	dep	regimes	340:346	arg1	nitrogen					361:368	[initial low nitrogen	348:368	three nitrogen supply regimes [initial low nitrogen (ILN)	318:374	We subjected the marine diatom Odontella aurita to three nitrogen supply regimes [initial low nitrogen (ILN), initial high nitrogen (IHN), and initial high nitrogen plus supplementary nitrogen (SN)] to investigate the accumulation of three high-value bioactive components: fucoxanthin, chrysolaminarin and eicosapentaenoic acid (EPA).
29958771	2	62	theme	bioactive	518:526	arg1	components					528:537	three high-value bioactive components	501:537	three high-value bioactive components: fucoxanthin, chrysolaminarin and eicosapentaenoic acid (EPA)	501:599	We subjected the marine diatom Odontella aurita to three nitrogen supply regimes [initial low nitrogen (ILN), initial high nitrogen (IHN), and initial high nitrogen plus supplementary nitrogen (SN)] to investigate the accumulation of three high-value bioactive components: fucoxanthin, chrysolaminarin and eicosapentaenoic acid (EPA).
29958771	2	62	theme	bioactive	518:526	arg1	chrysolaminarin					553:567	chrysolaminarin	553:567	chrysolaminarin	553:567	We subjected the marine diatom Odontella aurita to three nitrogen supply regimes [initial low nitrogen (ILN), initial high nitrogen (IHN), and initial high nitrogen plus supplementary nitrogen (SN)] to investigate the accumulation of three high-value bioactive components: fucoxanthin, chrysolaminarin and eicosapentaenoic acid (EPA).
29958771	2	62	theme	bioactive	518:526	arg1	acid					590:593	eicosapentaenoic acid	573:593	eicosapentaenoic acid (EPA)	573:599	We subjected the marine diatom Odontella aurita to three nitrogen supply regimes [initial low nitrogen (ILN), initial high nitrogen (IHN), and initial high nitrogen plus supplementary nitrogen (SN)] to investigate the accumulation of three high-value bioactive components: fucoxanthin, chrysolaminarin and eicosapentaenoic acid (EPA).
29958771	2	62	theme	bioactive	518:526	arg1	fucoxanthin					540:550	fucoxanthin	540:550	fucoxanthin	540:550	We subjected the marine diatom Odontella aurita to three nitrogen supply regimes [initial low nitrogen (ILN), initial high nitrogen (IHN), and initial high nitrogen plus supplementary nitrogen (SN)] to investigate the accumulation of three high-value bioactive components: fucoxanthin, chrysolaminarin and eicosapentaenoic acid (EPA).
29958771	3	63	theme	SN	616:617	arg1	conditions					619:628	SN conditions	616:628	SN conditions	616:628	We found that SN conditions maximized fucoxanthin accumulation: a maximum productivity of 6.01 mg L-1 d-1 was obtained, a 4.32-fold and 1.42-fold increase over production in the ILN and IHN groups, respectively.
29958771	6	64	theme	supply	1103:1108	arg1	regimes					1110:1116	the different nitrogen supply regimes	1080:1116	the different nitrogen supply regimes	1080:1116	Variations in fatty acid composition across the different nitrogen supply regimes indicated that EPA primarily accumulated in the glycolipids, especially when nitrogen supply was sufficient.
29958771	8	65	theme	overall	1389:1395	arg1	content					1397:1403	overall content	1389:1403	overall content of either compound	1389:1422	However, IHN conditions did not maximize overall content of either compound.
29958771	3	66	theme	ILN	780:782	arg1	groups					792:797	the ILN and IHN groups	776:797	the ILN and IHN groups	776:797	We found that SN conditions maximized fucoxanthin accumulation: a maximum productivity of 6.01 mg L-1 d-1 was obtained, a 4.32-fold and 1.42-fold increase over production in the ILN and IHN groups, respectively.
29958771	2	67	dep	components	528:537	arg1	components					528:537	three high-value bioactive components	501:537	three high-value bioactive components: fucoxanthin, chrysolaminarin and eicosapentaenoic acid (EPA)	501:599	We subjected the marine diatom Odontella aurita to three nitrogen supply regimes [initial low nitrogen (ILN), initial high nitrogen (IHN), and initial high nitrogen plus supplementary nitrogen (SN)] to investigate the accumulation of three high-value bioactive components: fucoxanthin, chrysolaminarin and eicosapentaenoic acid (EPA).
29958771	2	67	dep	components	528:537	arg1	EPA					596:598	EPA	596:598	EPA	596:598	We subjected the marine diatom Odontella aurita to three nitrogen supply regimes [initial low nitrogen (ILN), initial high nitrogen (IHN), and initial high nitrogen plus supplementary nitrogen (SN)] to investigate the accumulation of three high-value bioactive components: fucoxanthin, chrysolaminarin and eicosapentaenoic acid (EPA).
29958771	2	67	dep	components	528:537	arg1	chrysolaminarin					553:567	chrysolaminarin	553:567	chrysolaminarin	553:567	We subjected the marine diatom Odontella aurita to three nitrogen supply regimes [initial low nitrogen (ILN), initial high nitrogen (IHN), and initial high nitrogen plus supplementary nitrogen (SN)] to investigate the accumulation of three high-value bioactive components: fucoxanthin, chrysolaminarin and eicosapentaenoic acid (EPA).
29958771	2	67	dep	components	528:537	arg1	acid					590:593	eicosapentaenoic acid	573:593	eicosapentaenoic acid (EPA)	573:599	We subjected the marine diatom Odontella aurita to three nitrogen supply regimes [initial low nitrogen (ILN), initial high nitrogen (IHN), and initial high nitrogen plus supplementary nitrogen (SN)] to investigate the accumulation of three high-value bioactive components: fucoxanthin, chrysolaminarin and eicosapentaenoic acid (EPA).
29958771	2	67	dep	components	528:537	arg1	fucoxanthin					540:550	fucoxanthin	540:550	fucoxanthin	540:550	We subjected the marine diatom Odontella aurita to three nitrogen supply regimes [initial low nitrogen (ILN), initial high nitrogen (IHN), and initial high nitrogen plus supplementary nitrogen (SN)] to investigate the accumulation of three high-value bioactive components: fucoxanthin, chrysolaminarin and eicosapentaenoic acid (EPA).
29958771	5	68	theme	dry	967:969	arg1	DW					979:980	DW	979:980	DW	979:980	Chrysolaminarin content rose to 60.33% of dry weight (DW) in the ILN group, and 46.27% of DW in the IHN group.
29958771	5	68	theme	dry	967:969	arg1	weight					971:976	dry weight	967:976	dry weight (DW) in the ILN group	967:998	Chrysolaminarin content rose to 60.33% of dry weight (DW) in the ILN group, and 46.27% of DW in the IHN group.
29958771	9	69	theme	variety	1506:1512	arg1	producer					1492:1499	a producer	1490:1499	a producer of a variety of bioactive products	1490:1534	Our results demonstrated that O. aurita is potentially useful as a producer of a variety of bioactive products; the compounds produced by this species can be controlled by altering the nitrogen supply.
29958771	0	70	theme	acid	65:68	arg1	Production					0:9	Production	0:9	Production of fucoxanthin, chrysolaminarin, and eicosapentaenoic acid by Odontella	0:81	Production of fucoxanthin, chrysolaminarin, and eicosapentaenoic acid by Odontella aurita under different nitrogen supply regimes.
29958771	6	71	theme	fatty	1050:1054	arg1	composition					1061:1071	fatty acid composition	1050:1071	fatty acid composition across the different nitrogen supply regimes	1050:1116	Variations in fatty acid composition across the different nitrogen supply regimes indicated that EPA primarily accumulated in the glycolipids, especially when nitrogen supply was sufficient.
29958771	5	72	theme	weight	971:976	arg1	DW					979:980	DW	979:980	DW	979:980	Chrysolaminarin content rose to 60.33% of dry weight (DW) in the ILN group, and 46.27% of DW in the IHN group.
29958771	5	72	theme	weight	971:976	arg1	%					962:962	60.33%	957:962	60.33% of dry weight (DW) in the ILN group	957:998	Chrysolaminarin content rose to 60.33% of dry weight (DW) in the ILN group, and 46.27% of DW in the IHN group.
29958771	5	72	theme	weight	971:976	arg1	weight					971:976	dry weight	967:976	dry weight (DW) in the ILN group	967:998	Chrysolaminarin content rose to 60.33% of dry weight (DW) in the ILN group, and 46.27% of DW in the IHN group.
29958771	5	72	theme	weight	971:976	arg1	%					1010:1010	46.27%	1005:1010	46.27% of DW	1005:1016	Chrysolaminarin content rose to 60.33% of dry weight (DW) in the ILN group, and 46.27% of DW in the IHN group.
29958771	5	72	theme	weight	971:976	arg1	DW					1015:1016	DW	1015:1016	DW	1015:1016	Chrysolaminarin content rose to 60.33% of dry weight (DW) in the ILN group, and 46.27% of DW in the IHN group.
29807225	2	0	with	modulation	408:417	arg1	prebiotics					456:465	prebiotics	456:465	prebiotics	456:465	Several lines of evidence suggest that maternal gut microbiota influences in utero fetal development via modulation of host's microbial composition with prebiotics.
29807225	7	1	theme	placental	1430:1438	arg1	weight					1440:1445	fetal and placental weight	1420:1445	fetal and placental weight on GD 19	1420:1454	In addition, IN supplements improved fetal and placental weight on GD 19.
29807225	8	2	theme	maternal	1611:1618	arg1	cerebellum					1643:1652	cerebellum	1643:1652	cerebellum	1643:1652	IN diminished gestational ROT-induced increased reactive oxygen species levels, protein and lipid peroxidation biomarkers, and cholinesterase activity in maternal brain regions (cortex, cerebellum, and striatum) and fetal brain.
29807225	8	2	theme	maternal	1611:1618	arg1	striatum					1659:1666	striatum	1659:1666	striatum	1659:1666	IN diminished gestational ROT-induced increased reactive oxygen species levels, protein and lipid peroxidation biomarkers, and cholinesterase activity in maternal brain regions (cortex, cerebellum, and striatum) and fetal brain.
29807225	8	2	theme	maternal	1611:1618	arg1	cortex					1635:1640	cortex	1635:1640	cortex	1635:1640	IN diminished gestational ROT-induced increased reactive oxygen species levels, protein and lipid peroxidation biomarkers, and cholinesterase activity in maternal brain regions (cortex, cerebellum, and striatum) and fetal brain.
29807225	8	2	theme	maternal	1611:1618	arg1	regions					1626:1632	maternal brain regions	1611:1632	maternal brain regions (cortex, cerebellum, and striatum)	1611:1667	IN diminished gestational ROT-induced increased reactive oxygen species levels, protein and lipid peroxidation biomarkers, and cholinesterase activity in maternal brain regions (cortex, cerebellum, and striatum) and fetal brain.
29807225	7	3	theme	fetal	1420:1424	arg1	weight					1440:1445	fetal and placental weight	1420:1445	fetal and placental weight on GD 19	1420:1454	In addition, IN supplements improved fetal and placental weight on GD 19.
29807225	10	4	theme	indigestible	1896:1907	arg1	oligosaccharides					1909:1924	indigestible oligosaccharides	1896:1924	indigestible oligosaccharides	1896:1924	These data suggest a potential role for indigestible oligosaccharides in reducing oxidative stress-mediated developmental origins of neurodegenerative disorders.
29807225	5	5	from	inulin	1140:1145	arg1	GD					1170:1171	GD 0-21	1170:1176	GD 0-21	1170:1176	Secondly, dams orally supplemented with inulin (2×/day, 2 g/kg/bw) on GD 0-21 were administered ROT (50 mg/kg, GD 6-19).
29807225	8	6	theme	fetal	1673:1677	arg1	brain					1679:1683	fetal brain	1673:1683	fetal brain	1673:1683	IN diminished gestational ROT-induced increased reactive oxygen species levels, protein and lipid peroxidation biomarkers, and cholinesterase activity in maternal brain regions (cortex, cerebellum, and striatum) and fetal brain.
29807225	4	7	dep	10	811:812	arg1	low					815:817	low	815:817	low	815:817	Initially, pregnant Sprague-Dawley rats were gavaged during gestational days (GDs) 6-19 with 0 (control), 10 (low), 30 (mid) or 50 (high) mg/kg bw/day of ROT to recapitulate developmental effects on general fetotoxicity (assessed by the number of fetuses, fetal body and placental weights), markers of oxidative stress and cholinergic activities in maternal brain regions and whole fetal-brain.
29807225	4	8	theme	whole	1081:1085	arg1	fetal-brain					1087:1097	whole fetal-brain	1081:1097	whole fetal-brain	1081:1097	Initially, pregnant Sprague-Dawley rats were gavaged during gestational days (GDs) 6-19 with 0 (control), 10 (low), 30 (mid) or 50 (high) mg/kg bw/day of ROT to recapitulate developmental effects on general fetotoxicity (assessed by the number of fetuses, fetal body and placental weights), markers of oxidative stress and cholinergic activities in maternal brain regions and whole fetal-brain.
29807225	10	9	theme	developmental	1964:1976	arg1	origins					1978:1984	oxidative stress-mediated developmental origins	1938:1984	oxidative stress-mediated developmental origins of neurodegenerative disorders	1938:2015	These data suggest a potential role for indigestible oligosaccharides in reducing oxidative stress-mediated developmental origins of neurodegenerative disorders.
29807225	10	10	theme	oxidative	1938:1946	arg1	origins					1978:1984	oxidative stress-mediated developmental origins	1938:1984	oxidative stress-mediated developmental origins of neurodegenerative disorders	1938:2015	These data suggest a potential role for indigestible oligosaccharides in reducing oxidative stress-mediated developmental origins of neurodegenerative disorders.
29807225	4	11	dep	fetotoxicity	912:923	arg1	assessed					926:933	assessed	926:933	assessed by the number of fetuses, fetal body and placental weights	926:992	Initially, pregnant Sprague-Dawley rats were gavaged during gestational days (GDs) 6-19 with 0 (control), 10 (low), 30 (mid) or 50 (high) mg/kg bw/day of ROT to recapitulate developmental effects on general fetotoxicity (assessed by the number of fetuses, fetal body and placental weights), markers of oxidative stress and cholinergic activities in maternal brain regions and whole fetal-brain.
29807225	4	12	from	effects	893:899	arg1	fetotoxicity					912:923	general fetotoxicity	904:923	general fetotoxicity (assessed by the number of fetuses, fetal body and placental weights)	904:993	Initially, pregnant Sprague-Dawley rats were gavaged during gestational days (GDs) 6-19 with 0 (control), 10 (low), 30 (mid) or 50 (high) mg/kg bw/day of ROT to recapitulate developmental effects on general fetotoxicity (assessed by the number of fetuses, fetal body and placental weights), markers of oxidative stress and cholinergic activities in maternal brain regions and whole fetal-brain.
29807225	4	12	from	effects	893:899	arg1	markers					996:1002	markers	996:1002	markers of oxidative stress	996:1022	Initially, pregnant Sprague-Dawley rats were gavaged during gestational days (GDs) 6-19 with 0 (control), 10 (low), 30 (mid) or 50 (high) mg/kg bw/day of ROT to recapitulate developmental effects on general fetotoxicity (assessed by the number of fetuses, fetal body and placental weights), markers of oxidative stress and cholinergic activities in maternal brain regions and whole fetal-brain.
29807225	4	12	from	effects	893:899	arg1	activities					1040:1049	cholinergic activities	1028:1049	cholinergic activities	1028:1049	Initially, pregnant Sprague-Dawley rats were gavaged during gestational days (GDs) 6-19 with 0 (control), 10 (low), 30 (mid) or 50 (high) mg/kg bw/day of ROT to recapitulate developmental effects on general fetotoxicity (assessed by the number of fetuses, fetal body and placental weights), markers of oxidative stress and cholinergic activities in maternal brain regions and whole fetal-brain.
29807225	4	12	from	effects	893:899	arg1	regions					1069:1075	maternal brain regions	1054:1075	maternal brain regions	1054:1075	Initially, pregnant Sprague-Dawley rats were gavaged during gestational days (GDs) 6-19 with 0 (control), 10 (low), 30 (mid) or 50 (high) mg/kg bw/day of ROT to recapitulate developmental effects on general fetotoxicity (assessed by the number of fetuses, fetal body and placental weights), markers of oxidative stress and cholinergic activities in maternal brain regions and whole fetal-brain.
29807225	4	12	from	effects	893:899	arg1	fetal-brain					1087:1097	whole fetal-brain	1081:1097	whole fetal-brain	1081:1097	Initially, pregnant Sprague-Dawley rats were gavaged during gestational days (GDs) 6-19 with 0 (control), 10 (low), 30 (mid) or 50 (high) mg/kg bw/day of ROT to recapitulate developmental effects on general fetotoxicity (assessed by the number of fetuses, fetal body and placental weights), markers of oxidative stress and cholinergic activities in maternal brain regions and whole fetal-brain.
29807225	3	13	theme	brain	588:592	arg1	response					604:611	brain oxidative response	588:611	brain oxidative response	588:611	Hence we examined the hypothesis if inulin (IN) supplements during pregnancy in rats possess the potential to alleviate brain oxidative response and mitochondrial deficits employing a developmental model of rotenone (ROT) neurotoxicity.
29807225	1	14	theme	immature	221:228	arg1	brain					230:234	the immature brain	217:234	the immature brain	217:234	Environmental insults including pesticide exposure and their entry into the immature brain are of increased concern due to their developmental neurotoxicity.
29807225	4	15	theme	maternal	1054:1061	arg1	regions					1069:1075	maternal brain regions	1054:1075	maternal brain regions	1054:1075	Initially, pregnant Sprague-Dawley rats were gavaged during gestational days (GDs) 6-19 with 0 (control), 10 (low), 30 (mid) or 50 (high) mg/kg bw/day of ROT to recapitulate developmental effects on general fetotoxicity (assessed by the number of fetuses, fetal body and placental weights), markers of oxidative stress and cholinergic activities in maternal brain regions and whole fetal-brain.
29807225	10	16	theme	disorders	2007:2015	arg1	origins					1978:1984	oxidative stress-mediated developmental origins	1938:1984	oxidative stress-mediated developmental origins of neurodegenerative disorders	1938:2015	These data suggest a potential role for indigestible oligosaccharides in reducing oxidative stress-mediated developmental origins of neurodegenerative disorders.
29807225	8	17	dep	protein	1537:1543	arg1	biomarkers					1568:1577	biomarkers	1568:1577	biomarkers	1568:1577	IN diminished gestational ROT-induced increased reactive oxygen species levels, protein and lipid peroxidation biomarkers, and cholinesterase activity in maternal brain regions (cortex, cerebellum, and striatum) and fetal brain.
29807225	7	18	from	weight	1440:1445	arg1	GD					1450:1451	GD 19	1450:1454	GD 19	1450:1454	In addition, IN supplements improved fetal and placental weight on GD 19.
29807225	3	19	theme	mitochondrial	617:629	arg1	deficits					631:638	mitochondrial deficits	617:638	mitochondrial deficits employing a developmental model of rotenone (ROT) neurotoxicity	617:702	Hence we examined the hypothesis if inulin (IN) supplements during pregnancy in rats possess the potential to alleviate brain oxidative response and mitochondrial deficits employing a developmental model of rotenone (ROT) neurotoxicity.
29807225	4	20	theme	ROT	859:861	arg1	bw/day					849:854	0 (control), 10 (low), 30 (mid) or 50 (high) mg/kg bw/day	798:854	0 (control), 10 (low), 30 (mid) or 50 (high) mg/kg bw/day of ROT to recapitulate developmental effects on general fetotoxicity (assessed by the number of fetuses, fetal body and placental weights), markers of oxidative stress and cholinergic activities in maternal brain regions and whole fetal-brain	798:1097	Initially, pregnant Sprague-Dawley rats were gavaged during gestational days (GDs) 6-19 with 0 (control), 10 (low), 30 (mid) or 50 (high) mg/kg bw/day of ROT to recapitulate developmental effects on general fetotoxicity (assessed by the number of fetuses, fetal body and placental weights), markers of oxidative stress and cholinergic activities in maternal brain regions and whole fetal-brain.
29807225	4	21	theme	days	777:780	arg1	6-19					788:791	gestational days (GDs) 6-19	765:791	gestational days (GDs) 6-19	765:791	Initially, pregnant Sprague-Dawley rats were gavaged during gestational days (GDs) 6-19 with 0 (control), 10 (low), 30 (mid) or 50 (high) mg/kg bw/day of ROT to recapitulate developmental effects on general fetotoxicity (assessed by the number of fetuses, fetal body and placental weights), markers of oxidative stress and cholinergic activities in maternal brain regions and whole fetal-brain.
29807225	1	22	theme	Environmental	145:157	arg1	entry					206:210	their entry	200:210	their entry into the immature brain	200:234	Environmental insults including pesticide exposure and their entry into the immature brain are of increased concern due to their developmental neurotoxicity.
29807225	1	22	theme	Environmental	145:157	arg1	insults					159:165	Environmental insults	145:165	Environmental insults including pesticide exposure and their entry into the immature brain	145:234	Environmental insults including pesticide exposure and their entry into the immature brain are of increased concern due to their developmental neurotoxicity.
29807225	1	22	theme	Environmental	145:157	arg1	exposure					187:194	pesticide exposure	177:194	pesticide exposure	177:194	Environmental insults including pesticide exposure and their entry into the immature brain are of increased concern due to their developmental neurotoxicity.
29807225	4	23	theme	Sprague-Dawley	725:738	arg1	rats					740:743	pregnant Sprague-Dawley rats	716:743	pregnant Sprague-Dawley rats	716:743	Initially, pregnant Sprague-Dawley rats were gavaged during gestational days (GDs) 6-19 with 0 (control), 10 (low), 30 (mid) or 50 (high) mg/kg bw/day of ROT to recapitulate developmental effects on general fetotoxicity (assessed by the number of fetuses, fetal body and placental weights), markers of oxidative stress and cholinergic activities in maternal brain regions and whole fetal-brain.
29807225	6	24	theme	exploratory-related	1325:1343	arg1	behavior					1345:1352	improved exploratory-related behavior	1316:1352	improved exploratory-related behavior among ROT administered rats	1316:1380	IN supplements increased maternal cecal bacterial numbers that significantly corresponded with improved exploratory-related behavior among ROT administered rats.
29807225	3	25	theme	developmental	652:664	arg1	model					666:670	a developmental model	650:670	a developmental model of rotenone (ROT) neurotoxicity	650:702	Hence we examined the hypothesis if inulin (IN) supplements during pregnancy in rats possess the potential to alleviate brain oxidative response and mitochondrial deficits employing a developmental model of rotenone (ROT) neurotoxicity.
29807225	9	26	theme	maternal	1703:1710	arg1	cortex					1712:1717	the maternal cortex	1699:1717	the maternal cortex	1699:1717	Moreover, in the maternal cortex, mitochondrial assessment revealed IN protected against ROT-induced reduction in NADH cytochrome c oxidoreductase and ATPase activities.
29807225	5	27	dep	inulin	1140:1145	arg1	2 g/kg/bw					1156:1164	2 g/kg/bw	1156:1164	2 g/kg/bw	1156:1164	Secondly, dams orally supplemented with inulin (2×/day, 2 g/kg/bw) on GD 0-21 were administered ROT (50 mg/kg, GD 6-19).
29807225	5	27	dep	inulin	1140:1145	arg1	2×/day					1148:1153	2×/day	1148:1153	2×/day	1148:1153	Secondly, dams orally supplemented with inulin (2×/day, 2 g/kg/bw) on GD 0-21 were administered ROT (50 mg/kg, GD 6-19).
29807225	4	28	theme	general	904:910	arg1	fetotoxicity					912:923	general fetotoxicity	904:923	general fetotoxicity (assessed by the number of fetuses, fetal body and placental weights)	904:993	Initially, pregnant Sprague-Dawley rats were gavaged during gestational days (GDs) 6-19 with 0 (control), 10 (low), 30 (mid) or 50 (high) mg/kg bw/day of ROT to recapitulate developmental effects on general fetotoxicity (assessed by the number of fetuses, fetal body and placental weights), markers of oxidative stress and cholinergic activities in maternal brain regions and whole fetal-brain.
29807225	4	29	theme	0	798:798	arg1	bw/day					849:854	0 (control), 10 (low), 30 (mid) or 50 (high) mg/kg bw/day	798:854	0 (control), 10 (low), 30 (mid) or 50 (high) mg/kg bw/day of ROT to recapitulate developmental effects on general fetotoxicity (assessed by the number of fetuses, fetal body and placental weights), markers of oxidative stress and cholinergic activities in maternal brain regions and whole fetal-brain	798:1097	Initially, pregnant Sprague-Dawley rats were gavaged during gestational days (GDs) 6-19 with 0 (control), 10 (low), 30 (mid) or 50 (high) mg/kg bw/day of ROT to recapitulate developmental effects on general fetotoxicity (assessed by the number of fetuses, fetal body and placental weights), markers of oxidative stress and cholinergic activities in maternal brain regions and whole fetal-brain.
29807225	0	30	theme	Oral	0:3	arg1	supplements					5:15	Oral supplements	0:15	Oral supplements of inulin during gestation	0:42	Oral supplements of inulin during gestation offsets rotenone-induced oxidative impairments and neurotoxicity in maternal and prenatal rat brain.
29807225	4	31	theme	weights	986:992	arg1	number					942:947	the number	938:947	the number of fetuses, fetal body and placental weights	938:992	Initially, pregnant Sprague-Dawley rats were gavaged during gestational days (GDs) 6-19 with 0 (control), 10 (low), 30 (mid) or 50 (high) mg/kg bw/day of ROT to recapitulate developmental effects on general fetotoxicity (assessed by the number of fetuses, fetal body and placental weights), markers of oxidative stress and cholinergic activities in maternal brain regions and whole fetal-brain.
29807225	8	32	theme	reactive	1505:1512	arg1	species					1521:1527	reactive oxygen species	1505:1527	reactive oxygen species levels	1505:1534	IN diminished gestational ROT-induced increased reactive oxygen species levels, protein and lipid peroxidation biomarkers, and cholinesterase activity in maternal brain regions (cortex, cerebellum, and striatum) and fetal brain.
29807225	2	33	dep	in	377:378	arg1	utero					380:384	utero	380:384	utero	380:384	Several lines of evidence suggest that maternal gut microbiota influences in utero fetal development via modulation of host's microbial composition with prebiotics.
29807225	3	34	dep	rats	548:551	arg1	possess					553:559	possess	553:559	possess the potential to alleviate brain oxidative response and mitochondrial deficits employing a developmental model of rotenone (ROT) neurotoxicity	553:702	Hence we examined the hypothesis if inulin (IN) supplements during pregnancy in rats possess the potential to alleviate brain oxidative response and mitochondrial deficits employing a developmental model of rotenone (ROT) neurotoxicity.
29807225	4	35	theme	fetuses	952:958	arg1	number					942:947	the number	938:947	the number of fetuses, fetal body and placental weights	938:992	Initially, pregnant Sprague-Dawley rats were gavaged during gestational days (GDs) 6-19 with 0 (control), 10 (low), 30 (mid) or 50 (high) mg/kg bw/day of ROT to recapitulate developmental effects on general fetotoxicity (assessed by the number of fetuses, fetal body and placental weights), markers of oxidative stress and cholinergic activities in maternal brain regions and whole fetal-brain.
29807225	8	36	theme	species	1521:1527	arg1	levels					1529:1534	reactive oxygen species levels	1505:1534	reactive oxygen species levels	1505:1534	IN diminished gestational ROT-induced increased reactive oxygen species levels, protein and lipid peroxidation biomarkers, and cholinesterase activity in maternal brain regions (cortex, cerebellum, and striatum) and fetal brain.
29807225	2	37	theme	composition	439:449	arg1	modulation					408:417	modulation	408:417	modulation of host's microbial composition with prebiotics	408:465	Several lines of evidence suggest that maternal gut microbiota influences in utero fetal development via modulation of host's microbial composition with prebiotics.
29807225	6	38	theme	administered	1364:1375	arg1	rats					1377:1380	ROT administered rats	1360:1380	ROT administered rats	1360:1380	IN supplements increased maternal cecal bacterial numbers that significantly corresponded with improved exploratory-related behavior among ROT administered rats.
29807225	6	39	dep	administered	1364:1375	arg1	ROT					1360:1362	ROT	1360:1362	ROT	1360:1362	IN supplements increased maternal cecal bacterial numbers that significantly corresponded with improved exploratory-related behavior among ROT administered rats.
29807225	4	40	theme	fetal	961:965	arg1	body					967:970	fetal body	961:970	fetal body	961:970	Initially, pregnant Sprague-Dawley rats were gavaged during gestational days (GDs) 6-19 with 0 (control), 10 (low), 30 (mid) or 50 (high) mg/kg bw/day of ROT to recapitulate developmental effects on general fetotoxicity (assessed by the number of fetuses, fetal body and placental weights), markers of oxidative stress and cholinergic activities in maternal brain regions and whole fetal-brain.
29807225	0	41	theme	oxidative	69:77	arg1	impairments					79:89	rotenone-induced oxidative impairments	52:89	rotenone-induced oxidative impairments	52:89	Oral supplements of inulin during gestation offsets rotenone-induced oxidative impairments and neurotoxicity in maternal and prenatal rat brain.
29807225	4	42	theme	30	821:822	arg1	bw/day					849:854	0 (control), 10 (low), 30 (mid) or 50 (high) mg/kg bw/day	798:854	0 (control), 10 (low), 30 (mid) or 50 (high) mg/kg bw/day of ROT to recapitulate developmental effects on general fetotoxicity (assessed by the number of fetuses, fetal body and placental weights), markers of oxidative stress and cholinergic activities in maternal brain regions and whole fetal-brain	798:1097	Initially, pregnant Sprague-Dawley rats were gavaged during gestational days (GDs) 6-19 with 0 (control), 10 (low), 30 (mid) or 50 (high) mg/kg bw/day of ROT to recapitulate developmental effects on general fetotoxicity (assessed by the number of fetuses, fetal body and placental weights), markers of oxidative stress and cholinergic activities in maternal brain regions and whole fetal-brain.
29807225	2	43	theme	in	377:378	arg1	development					392:402	in utero fetal development	377:402	in utero fetal development	377:402	Several lines of evidence suggest that maternal gut microbiota influences in utero fetal development via modulation of host's microbial composition with prebiotics.
29807225	4	44	theme	stress	1017:1022	arg1	fetotoxicity					912:923	general fetotoxicity	904:923	general fetotoxicity (assessed by the number of fetuses, fetal body and placental weights)	904:993	Initially, pregnant Sprague-Dawley rats were gavaged during gestational days (GDs) 6-19 with 0 (control), 10 (low), 30 (mid) or 50 (high) mg/kg bw/day of ROT to recapitulate developmental effects on general fetotoxicity (assessed by the number of fetuses, fetal body and placental weights), markers of oxidative stress and cholinergic activities in maternal brain regions and whole fetal-brain.
29807225	4	44	theme	stress	1017:1022	arg1	markers					996:1002	markers	996:1002	markers of oxidative stress	996:1022	Initially, pregnant Sprague-Dawley rats were gavaged during gestational days (GDs) 6-19 with 0 (control), 10 (low), 30 (mid) or 50 (high) mg/kg bw/day of ROT to recapitulate developmental effects on general fetotoxicity (assessed by the number of fetuses, fetal body and placental weights), markers of oxidative stress and cholinergic activities in maternal brain regions and whole fetal-brain.
29807225	4	44	theme	stress	1017:1022	arg1	activities					1040:1049	cholinergic activities	1028:1049	cholinergic activities	1028:1049	Initially, pregnant Sprague-Dawley rats were gavaged during gestational days (GDs) 6-19 with 0 (control), 10 (low), 30 (mid) or 50 (high) mg/kg bw/day of ROT to recapitulate developmental effects on general fetotoxicity (assessed by the number of fetuses, fetal body and placental weights), markers of oxidative stress and cholinergic activities in maternal brain regions and whole fetal-brain.
29807225	8	45	theme	brain	1620:1624	arg1	cerebellum					1643:1652	cerebellum	1643:1652	cerebellum	1643:1652	IN diminished gestational ROT-induced increased reactive oxygen species levels, protein and lipid peroxidation biomarkers, and cholinesterase activity in maternal brain regions (cortex, cerebellum, and striatum) and fetal brain.
29807225	8	45	theme	brain	1620:1624	arg1	striatum					1659:1666	striatum	1659:1666	striatum	1659:1666	IN diminished gestational ROT-induced increased reactive oxygen species levels, protein and lipid peroxidation biomarkers, and cholinesterase activity in maternal brain regions (cortex, cerebellum, and striatum) and fetal brain.
29807225	8	45	theme	brain	1620:1624	arg1	cortex					1635:1640	cortex	1635:1640	cortex	1635:1640	IN diminished gestational ROT-induced increased reactive oxygen species levels, protein and lipid peroxidation biomarkers, and cholinesterase activity in maternal brain regions (cortex, cerebellum, and striatum) and fetal brain.
29807225	8	45	theme	brain	1620:1624	arg1	regions					1626:1632	maternal brain regions	1611:1632	maternal brain regions (cortex, cerebellum, and striatum)	1611:1667	IN diminished gestational ROT-induced increased reactive oxygen species levels, protein and lipid peroxidation biomarkers, and cholinesterase activity in maternal brain regions (cortex, cerebellum, and striatum) and fetal brain.
29807225	4	46	theme	cholinergic	1028:1038	arg1	activities					1040:1049	cholinergic activities	1028:1049	cholinergic activities	1028:1049	Initially, pregnant Sprague-Dawley rats were gavaged during gestational days (GDs) 6-19 with 0 (control), 10 (low), 30 (mid) or 50 (high) mg/kg bw/day of ROT to recapitulate developmental effects on general fetotoxicity (assessed by the number of fetuses, fetal body and placental weights), markers of oxidative stress and cholinergic activities in maternal brain regions and whole fetal-brain.
29807225	9	47	theme	NADH	1800:1803	arg1	oxidoreductase					1818:1831	NADH cytochrome c oxidoreductase	1800:1831	NADH cytochrome c oxidoreductase	1800:1831	Moreover, in the maternal cortex, mitochondrial assessment revealed IN protected against ROT-induced reduction in NADH cytochrome c oxidoreductase and ATPase activities.
29807225	10	48	theme	potential	1877:1885	arg1	role					1887:1890	a potential role	1875:1890	a potential role for indigestible oligosaccharides in reducing oxidative stress-mediated developmental origins of neurodegenerative disorders	1875:2015	These data suggest a potential role for indigestible oligosaccharides in reducing oxidative stress-mediated developmental origins of neurodegenerative disorders.
29807225	2	49	theme	fetal	386:390	arg1	development					392:402	in utero fetal development	377:402	in utero fetal development	377:402	Several lines of evidence suggest that maternal gut microbiota influences in utero fetal development via modulation of host's microbial composition with prebiotics.
29807225	9	50	from	reduction	1787:1795	arg1	oxidoreductase					1818:1831	NADH cytochrome c oxidoreductase	1800:1831	NADH cytochrome c oxidoreductase	1800:1831	Moreover, in the maternal cortex, mitochondrial assessment revealed IN protected against ROT-induced reduction in NADH cytochrome c oxidoreductase and ATPase activities.
29807225	9	50	from	reduction	1787:1795	arg1	activities					1844:1853	ATPase activities	1837:1853	ATPase activities	1837:1853	Moreover, in the maternal cortex, mitochondrial assessment revealed IN protected against ROT-induced reduction in NADH cytochrome c oxidoreductase and ATPase activities.
29807225	4	51	theme	50	833:834	arg1	bw/day					849:854	0 (control), 10 (low), 30 (mid) or 50 (high) mg/kg bw/day	798:854	0 (control), 10 (low), 30 (mid) or 50 (high) mg/kg bw/day of ROT to recapitulate developmental effects on general fetotoxicity (assessed by the number of fetuses, fetal body and placental weights), markers of oxidative stress and cholinergic activities in maternal brain regions and whole fetal-brain	798:1097	Initially, pregnant Sprague-Dawley rats were gavaged during gestational days (GDs) 6-19 with 0 (control), 10 (low), 30 (mid) or 50 (high) mg/kg bw/day of ROT to recapitulate developmental effects on general fetotoxicity (assessed by the number of fetuses, fetal body and placental weights), markers of oxidative stress and cholinergic activities in maternal brain regions and whole fetal-brain.
29807225	8	52	theme	cholinesterase	1584:1597	arg1	activity					1599:1606	cholinesterase activity	1584:1606	cholinesterase activity	1584:1606	IN diminished gestational ROT-induced increased reactive oxygen species levels, protein and lipid peroxidation biomarkers, and cholinesterase activity in maternal brain regions (cortex, cerebellum, and striatum) and fetal brain.
29807225	1	53	theme	developmental	274:286	arg1	neurotoxicity					288:300	their developmental neurotoxicity	268:300	their developmental neurotoxicity	268:300	Environmental insults including pesticide exposure and their entry into the immature brain are of increased concern due to their developmental neurotoxicity.
29807225	2	54	theme	maternal	342:349	arg1	microbiota					355:364	maternal gut microbiota	342:364	maternal gut microbiota	342:364	Several lines of evidence suggest that maternal gut microbiota influences in utero fetal development via modulation of host's microbial composition with prebiotics.
29807225	4	55	theme	high	837:840	arg1	bw/day					849:854	0 (control), 10 (low), 30 (mid) or 50 (high) mg/kg bw/day	798:854	0 (control), 10 (low), 30 (mid) or 50 (high) mg/kg bw/day of ROT to recapitulate developmental effects on general fetotoxicity (assessed by the number of fetuses, fetal body and placental weights), markers of oxidative stress and cholinergic activities in maternal brain regions and whole fetal-brain	798:1097	Initially, pregnant Sprague-Dawley rats were gavaged during gestational days (GDs) 6-19 with 0 (control), 10 (low), 30 (mid) or 50 (high) mg/kg bw/day of ROT to recapitulate developmental effects on general fetotoxicity (assessed by the number of fetuses, fetal body and placental weights), markers of oxidative stress and cholinergic activities in maternal brain regions and whole fetal-brain.
29807225	2	56	theme	Several	303:309	arg1	lines					311:315	Several lines	303:315	Several lines of evidence	303:327	Several lines of evidence suggest that maternal gut microbiota influences in utero fetal development via modulation of host's microbial composition with prebiotics.
29807225	7	57	theme	IN	1396:1397	arg1	supplements					1399:1409	IN supplements	1396:1409	IN supplements	1396:1409	In addition, IN supplements improved fetal and placental weight on GD 19.
29807225	5	58	dep	ROT	1196:1198	arg1	50 mg/kg					1201:1208	50 mg/kg	1201:1208	50 mg/kg	1201:1208	Secondly, dams orally supplemented with inulin (2×/day, 2 g/kg/bw) on GD 0-21 were administered ROT (50 mg/kg, GD 6-19).
29807225	5	58	dep	ROT	1196:1198	arg1	6-19					1214:1217	GD 6-19	1211:1217	GD 6-19	1211:1217	Secondly, dams orally supplemented with inulin (2×/day, 2 g/kg/bw) on GD 0-21 were administered ROT (50 mg/kg, GD 6-19).
29807225	3	59	theme	oxidative	594:602	arg1	response					604:611	brain oxidative response	588:611	brain oxidative response	588:611	Hence we examined the hypothesis if inulin (IN) supplements during pregnancy in rats possess the potential to alleviate brain oxidative response and mitochondrial deficits employing a developmental model of rotenone (ROT) neurotoxicity.
29807225	10	60	theme	stress-mediated	1948:1962	arg1	origins					1978:1984	oxidative stress-mediated developmental origins	1938:1984	oxidative stress-mediated developmental origins of neurodegenerative disorders	1938:2015	These data suggest a potential role for indigestible oligosaccharides in reducing oxidative stress-mediated developmental origins of neurodegenerative disorders.
29807225	6	61	theme	bacterial	1261:1269	arg1	numbers					1271:1277	maternal cecal bacterial numbers	1246:1277	maternal cecal bacterial numbers that significantly corresponded with improved exploratory-related behavior among ROT administered rats	1246:1380	IN supplements increased maternal cecal bacterial numbers that significantly corresponded with improved exploratory-related behavior among ROT administered rats.
29807225	0	62	from	neurotoxicity	95:107	arg1	brain					138:142	maternal and prenatal rat brain	112:142	maternal and prenatal rat brain	112:142	Oral supplements of inulin during gestation offsets rotenone-induced oxidative impairments and neurotoxicity in maternal and prenatal rat brain.
29807225	4	63	theme	brain	1063:1067	arg1	regions					1069:1075	maternal brain regions	1054:1075	maternal brain regions	1054:1075	Initially, pregnant Sprague-Dawley rats were gavaged during gestational days (GDs) 6-19 with 0 (control), 10 (low), 30 (mid) or 50 (high) mg/kg bw/day of ROT to recapitulate developmental effects on general fetotoxicity (assessed by the number of fetuses, fetal body and placental weights), markers of oxidative stress and cholinergic activities in maternal brain regions and whole fetal-brain.
29807225	6	64	theme	maternal	1246:1253	arg1	numbers					1271:1277	maternal cecal bacterial numbers	1246:1277	maternal cecal bacterial numbers that significantly corresponded with improved exploratory-related behavior among ROT administered rats	1246:1380	IN supplements increased maternal cecal bacterial numbers that significantly corresponded with improved exploratory-related behavior among ROT administered rats.
29807225	9	65	theme	c	1816:1816	arg1	oxidoreductase					1818:1831	NADH cytochrome c oxidoreductase	1800:1831	NADH cytochrome c oxidoreductase	1800:1831	Moreover, in the maternal cortex, mitochondrial assessment revealed IN protected against ROT-induced reduction in NADH cytochrome c oxidoreductase and ATPase activities.
29807225	4	66	theme	developmental	879:891	arg1	effects					893:899	developmental effects	879:899	developmental effects on general fetotoxicity (assessed by the number of fetuses, fetal body and placental weights), markers of oxidative stress and cholinergic activities in maternal brain regions and whole fetal-brain	879:1097	Initially, pregnant Sprague-Dawley rats were gavaged during gestational days (GDs) 6-19 with 0 (control), 10 (low), 30 (mid) or 50 (high) mg/kg bw/day of ROT to recapitulate developmental effects on general fetotoxicity (assessed by the number of fetuses, fetal body and placental weights), markers of oxidative stress and cholinergic activities in maternal brain regions and whole fetal-brain.
29807225	0	67	from	impairments	79:89	arg1	brain					138:142	maternal and prenatal rat brain	112:142	maternal and prenatal rat brain	112:142	Oral supplements of inulin during gestation offsets rotenone-induced oxidative impairments and neurotoxicity in maternal and prenatal rat brain.
29807225	9	68	theme	ROT-induced	1775:1785	arg1	reduction					1787:1795	ROT-induced reduction	1775:1795	ROT-induced reduction in NADH cytochrome c oxidoreductase and ATPase activities	1775:1853	Moreover, in the maternal cortex, mitochondrial assessment revealed IN protected against ROT-induced reduction in NADH cytochrome c oxidoreductase and ATPase activities.
29807225	4	69	theme	mg/kg	843:847	arg1	bw/day					849:854	0 (control), 10 (low), 30 (mid) or 50 (high) mg/kg bw/day	798:854	0 (control), 10 (low), 30 (mid) or 50 (high) mg/kg bw/day of ROT to recapitulate developmental effects on general fetotoxicity (assessed by the number of fetuses, fetal body and placental weights), markers of oxidative stress and cholinergic activities in maternal brain regions and whole fetal-brain	798:1097	Initially, pregnant Sprague-Dawley rats were gavaged during gestational days (GDs) 6-19 with 0 (control), 10 (low), 30 (mid) or 50 (high) mg/kg bw/day of ROT to recapitulate developmental effects on general fetotoxicity (assessed by the number of fetuses, fetal body and placental weights), markers of oxidative stress and cholinergic activities in maternal brain regions and whole fetal-brain.
29807225	4	70	theme	gestational	765:775	arg1	GDs					783:785	GDs	783:785	GDs	783:785	Initially, pregnant Sprague-Dawley rats were gavaged during gestational days (GDs) 6-19 with 0 (control), 10 (low), 30 (mid) or 50 (high) mg/kg bw/day of ROT to recapitulate developmental effects on general fetotoxicity (assessed by the number of fetuses, fetal body and placental weights), markers of oxidative stress and cholinergic activities in maternal brain regions and whole fetal-brain.
29807225	4	70	theme	gestational	765:775	arg1	days					777:780	gestational days	765:780	gestational days (GDs) 6-19	765:791	Initially, pregnant Sprague-Dawley rats were gavaged during gestational days (GDs) 6-19 with 0 (control), 10 (low), 30 (mid) or 50 (high) mg/kg bw/day of ROT to recapitulate developmental effects on general fetotoxicity (assessed by the number of fetuses, fetal body and placental weights), markers of oxidative stress and cholinergic activities in maternal brain regions and whole fetal-brain.
29807225	5	71	theme	GD	1211:1212	arg1	50 mg/kg					1201:1208	50 mg/kg	1201:1208	50 mg/kg	1201:1208	Secondly, dams orally supplemented with inulin (2×/day, 2 g/kg/bw) on GD 0-21 were administered ROT (50 mg/kg, GD 6-19).
29807225	5	71	theme	GD	1211:1212	arg1	6-19					1214:1217	GD 6-19	1211:1217	GD 6-19	1211:1217	Secondly, dams orally supplemented with inulin (2×/day, 2 g/kg/bw) on GD 0-21 were administered ROT (50 mg/kg, GD 6-19).
29807225	8	72	dep	regions	1626:1632	arg1	cerebellum					1643:1652	cerebellum	1643:1652	cerebellum	1643:1652	IN diminished gestational ROT-induced increased reactive oxygen species levels, protein and lipid peroxidation biomarkers, and cholinesterase activity in maternal brain regions (cortex, cerebellum, and striatum) and fetal brain.
29807225	8	72	dep	regions	1626:1632	arg1	striatum					1659:1666	striatum	1659:1666	striatum	1659:1666	IN diminished gestational ROT-induced increased reactive oxygen species levels, protein and lipid peroxidation biomarkers, and cholinesterase activity in maternal brain regions (cortex, cerebellum, and striatum) and fetal brain.
29807225	8	72	dep	regions	1626:1632	arg1	cortex					1635:1640	cortex	1635:1640	cortex	1635:1640	IN diminished gestational ROT-induced increased reactive oxygen species levels, protein and lipid peroxidation biomarkers, and cholinesterase activity in maternal brain regions (cortex, cerebellum, and striatum) and fetal brain.
29807225	8	72	dep	regions	1626:1632	arg1	regions					1626:1632	maternal brain regions	1611:1632	maternal brain regions (cortex, cerebellum, and striatum)	1611:1667	IN diminished gestational ROT-induced increased reactive oxygen species levels, protein and lipid peroxidation biomarkers, and cholinesterase activity in maternal brain regions (cortex, cerebellum, and striatum) and fetal brain.
29807225	4	73	theme	pregnant	716:723	arg1	rats					740:743	pregnant Sprague-Dawley rats	716:743	pregnant Sprague-Dawley rats	716:743	Initially, pregnant Sprague-Dawley rats were gavaged during gestational days (GDs) 6-19 with 0 (control), 10 (low), 30 (mid) or 50 (high) mg/kg bw/day of ROT to recapitulate developmental effects on general fetotoxicity (assessed by the number of fetuses, fetal body and placental weights), markers of oxidative stress and cholinergic activities in maternal brain regions and whole fetal-brain.
29807225	6	74	theme	improved	1316:1323	arg1	behavior					1345:1352	improved exploratory-related behavior	1316:1352	improved exploratory-related behavior among ROT administered rats	1316:1380	IN supplements increased maternal cecal bacterial numbers that significantly corresponded with improved exploratory-related behavior among ROT administered rats.
29807225	1	75	theme	pesticide	177:185	arg1	exposure					187:194	pesticide exposure	177:194	pesticide exposure	177:194	Environmental insults including pesticide exposure and their entry into the immature brain are of increased concern due to their developmental neurotoxicity.
29807225	6	76	theme	cecal	1255:1259	arg1	numbers					1271:1277	maternal cecal bacterial numbers	1246:1277	maternal cecal bacterial numbers that significantly corresponded with improved exploratory-related behavior among ROT administered rats	1246:1380	IN supplements increased maternal cecal bacterial numbers that significantly corresponded with improved exploratory-related behavior among ROT administered rats.
29807225	0	77	theme	maternal	112:119	arg1	brain					138:142	maternal and prenatal rat brain	112:142	maternal and prenatal rat brain	112:142	Oral supplements of inulin during gestation offsets rotenone-induced oxidative impairments and neurotoxicity in maternal and prenatal rat brain.
29807225	2	78	theme	evidence	320:327	arg1	lines					311:315	Several lines	303:315	Several lines of evidence	303:327	Several lines of evidence suggest that maternal gut microbiota influences in utero fetal development via modulation of host's microbial composition with prebiotics.
29807225	9	79	theme	mitochondrial	1720:1732	arg1	assessment					1734:1743	mitochondrial assessment	1720:1743	mitochondrial assessment	1720:1743	Moreover, in the maternal cortex, mitochondrial assessment revealed IN protected against ROT-induced reduction in NADH cytochrome c oxidoreductase and ATPase activities.
29807225	0	80	theme	prenatal	125:132	arg1	brain					138:142	maternal and prenatal rat brain	112:142	maternal and prenatal rat brain	112:142	Oral supplements of inulin during gestation offsets rotenone-induced oxidative impairments and neurotoxicity in maternal and prenatal rat brain.
29807225	4	81	theme	body	967:970	arg1	number					942:947	the number	938:947	the number of fetuses, fetal body and placental weights	938:992	Initially, pregnant Sprague-Dawley rats were gavaged during gestational days (GDs) 6-19 with 0 (control), 10 (low), 30 (mid) or 50 (high) mg/kg bw/day of ROT to recapitulate developmental effects on general fetotoxicity (assessed by the number of fetuses, fetal body and placental weights), markers of oxidative stress and cholinergic activities in maternal brain regions and whole fetal-brain.
29807225	8	82	theme	oxygen	1514:1519	arg1	species					1521:1527	reactive oxygen species	1505:1527	reactive oxygen species levels	1505:1534	IN diminished gestational ROT-induced increased reactive oxygen species levels, protein and lipid peroxidation biomarkers, and cholinesterase activity in maternal brain regions (cortex, cerebellum, and striatum) and fetal brain.
29807225	0	83	theme	inulin	20:25	arg1	supplements					5:15	Oral supplements	0:15	Oral supplements of inulin during gestation	0:42	Oral supplements of inulin during gestation offsets rotenone-induced oxidative impairments and neurotoxicity in maternal and prenatal rat brain.
29807225	4	84	theme	placental	976:984	arg1	weights					986:992	placental weights	976:992	placental weights	976:992	Initially, pregnant Sprague-Dawley rats were gavaged during gestational days (GDs) 6-19 with 0 (control), 10 (low), 30 (mid) or 50 (high) mg/kg bw/day of ROT to recapitulate developmental effects on general fetotoxicity (assessed by the number of fetuses, fetal body and placental weights), markers of oxidative stress and cholinergic activities in maternal brain regions and whole fetal-brain.
29807225	3	85	theme	ROT	685:687	arg1	neurotoxicity					690:702	rotenone (ROT) neurotoxicity	675:702	rotenone (ROT) neurotoxicity	675:702	Hence we examined the hypothesis if inulin (IN) supplements during pregnancy in rats possess the potential to alleviate brain oxidative response and mitochondrial deficits employing a developmental model of rotenone (ROT) neurotoxicity.
29807225	3	86	theme	rotenone	675:682	arg1	neurotoxicity					690:702	rotenone (ROT) neurotoxicity	675:702	rotenone (ROT) neurotoxicity	675:702	Hence we examined the hypothesis if inulin (IN) supplements during pregnancy in rats possess the potential to alleviate brain oxidative response and mitochondrial deficits employing a developmental model of rotenone (ROT) neurotoxicity.
29807225	2	87	theme	microbial	429:437	arg1	composition					439:449	host's microbial composition	422:449	host's microbial composition	422:449	Several lines of evidence suggest that maternal gut microbiota influences in utero fetal development via modulation of host's microbial composition with prebiotics.
29807225	0	88	theme	rotenone-induced	52:67	arg1	impairments					79:89	rotenone-induced oxidative impairments	52:89	rotenone-induced oxidative impairments	52:89	Oral supplements of inulin during gestation offsets rotenone-induced oxidative impairments and neurotoxicity in maternal and prenatal rat brain.
29807225	9	89	theme	cytochrome	1805:1814	arg1	oxidoreductase					1818:1831	NADH cytochrome c oxidoreductase	1800:1831	NADH cytochrome c oxidoreductase	1800:1831	Moreover, in the maternal cortex, mitochondrial assessment revealed IN protected against ROT-induced reduction in NADH cytochrome c oxidoreductase and ATPase activities.
29807225	8	90	theme	lipid	1549:1553	arg1	peroxidation					1555:1566	lipid peroxidation	1549:1566	lipid peroxidation	1549:1566	IN diminished gestational ROT-induced increased reactive oxygen species levels, protein and lipid peroxidation biomarkers, and cholinesterase activity in maternal brain regions (cortex, cerebellum, and striatum) and fetal brain.
29807225	4	91	theme	oxidative	1007:1015	arg1	stress					1017:1022	oxidative stress	1007:1022	oxidative stress	1007:1022	Initially, pregnant Sprague-Dawley rats were gavaged during gestational days (GDs) 6-19 with 0 (control), 10 (low), 30 (mid) or 50 (high) mg/kg bw/day of ROT to recapitulate developmental effects on general fetotoxicity (assessed by the number of fetuses, fetal body and placental weights), markers of oxidative stress and cholinergic activities in maternal brain regions and whole fetal-brain.
29807225	3	92	theme	neurotoxicity	690:702	arg1	model					666:670	a developmental model	650:670	a developmental model of rotenone (ROT) neurotoxicity	650:702	Hence we examined the hypothesis if inulin (IN) supplements during pregnancy in rats possess the potential to alleviate brain oxidative response and mitochondrial deficits employing a developmental model of rotenone (ROT) neurotoxicity.
29807225	2	93	theme	gut	351:353	arg1	microbiota					355:364	maternal gut microbiota	342:364	maternal gut microbiota	342:364	Several lines of evidence suggest that maternal gut microbiota influences in utero fetal development via modulation of host's microbial composition with prebiotics.
29807225	1	94	theme	increased	243:251	arg1	concern					253:259	increased concern	243:259	increased concern due to their developmental neurotoxicity	243:300	Environmental insults including pesticide exposure and their entry into the immature brain are of increased concern due to their developmental neurotoxicity.
29807225	0	95	theme	rat	134:136	arg1	brain					138:142	maternal and prenatal rat brain	112:142	maternal and prenatal rat brain	112:142	Oral supplements of inulin during gestation offsets rotenone-induced oxidative impairments and neurotoxicity in maternal and prenatal rat brain.
29807225	9	96	theme	ATPase	1837:1842	arg1	activities					1844:1853	ATPase activities	1837:1853	ATPase activities	1837:1853	Moreover, in the maternal cortex, mitochondrial assessment revealed IN protected against ROT-induced reduction in NADH cytochrome c oxidoreductase and ATPase activities.
29807225	4	97	theme	10	811:812	arg1	bw/day					849:854	0 (control), 10 (low), 30 (mid) or 50 (high) mg/kg bw/day	798:854	0 (control), 10 (low), 30 (mid) or 50 (high) mg/kg bw/day of ROT to recapitulate developmental effects on general fetotoxicity (assessed by the number of fetuses, fetal body and placental weights), markers of oxidative stress and cholinergic activities in maternal brain regions and whole fetal-brain	798:1097	Initially, pregnant Sprague-Dawley rats were gavaged during gestational days (GDs) 6-19 with 0 (control), 10 (low), 30 (mid) or 50 (high) mg/kg bw/day of ROT to recapitulate developmental effects on general fetotoxicity (assessed by the number of fetuses, fetal body and placental weights), markers of oxidative stress and cholinergic activities in maternal brain regions and whole fetal-brain.
29807225	10	98	theme	neurodegenerative	1989:2005	arg1	disorders					2007:2015	neurodegenerative disorders	1989:2015	neurodegenerative disorders	1989:2015	These data suggest a potential role for indigestible oligosaccharides in reducing oxidative stress-mediated developmental origins of neurodegenerative disorders.
30625108	2	0	theme	rod-shaped	96:105	arg1	isolate					156:162	A Gram-stain-positive, rod-shaped, endospore-forming, motile and aerobic bacterial isolate	73:162	A Gram-stain-positive, rod-shaped, endospore-forming, motile and aerobic bacterial isolate	73:162	A Gram-stain-positive, rod-shaped, endospore-forming, motile and aerobic bacterial isolate, designated strain K2E09-144T, was obtained from animal faeces that were collected from a karst cave in Guizhou province, China.
30625108	5	1	theme	major	623:627	arg1	anteiso-C15:0					655:667	anteiso-C15:0	655:667	anteiso-C15:0	655:667	The major cellular fatty acids were anteiso-C15:0, iso-C16:0 and C16 : 0.
30625108	5	1	theme	major	623:627	arg1	acids					644:648	The major cellular fatty acids	619:648	The major cellular fatty acids	619:648	The major cellular fatty acids were anteiso-C15:0, iso-C16:0 and C16 : 0.
30625108	4	2	dep	related	537:543	arg1	similarity					599:608	16S rRNA gene sequence similarity	576:608	16S rRNA gene sequence similarity 97.0 %	576:615	Strain K2E09-144T was phylogenetically closely related to Cohnella nanjingensis D45T (16S rRNA gene sequence similarity 97.0 %).
30625108	3	3	theme	phylum	471:476	arg1	Firmicutes					478:487	the phylum Firmicutes	467:487	the phylum Firmicutes	467:487	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain K2E09-144T represents a novel member of the genus Cohnella within the family Paenibacillaceae of the phylum Firmicutes.
30625108	4	4	theme	Strain	490:495	arg1	K2E09-144T					497:506	Strain K2E09-144T	490:506	Strain K2E09-144T	490:506	Strain K2E09-144T was phylogenetically closely related to Cohnella nanjingensis D45T (16S rRNA gene sequence similarity 97.0 %).
30625108	5	5	theme	cellular	629:636	arg1	anteiso-C15:0					655:667	anteiso-C15:0	655:667	anteiso-C15:0	655:667	The major cellular fatty acids were anteiso-C15:0, iso-C16:0 and C16 : 0.
30625108	5	5	theme	cellular	629:636	arg1	acids					644:648	The major cellular fatty acids	619:648	The major cellular fatty acids	619:648	The major cellular fatty acids were anteiso-C15:0, iso-C16:0 and C16 : 0.
30625108	3	6	theme	Firmicutes	478:487	arg1	Paenibacillaceae					447:462	the family Paenibacillaceae	436:462	the family Paenibacillaceae of the phylum Firmicutes	436:487	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain K2E09-144T represents a novel member of the genus Cohnella within the family Paenibacillaceae of the phylum Firmicutes.
30625108	7	7	theme	unidentified	851:862	arg1	phospholipid					864:875	one unidentified phospholipid	847:875	one unidentified phospholipid	847:875	The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified phospholipid, four unidentified aminophospholipids, one glycolipid and one unidentified lipid.
30625108	8	8	theme	meso-diaminopimelic	1026:1044	arg1	acid					1046:1049	meso-diaminopimelic acid	1026:1049	meso-diaminopimelic acid	1026:1049	The isomer type of diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid.
30625108	8	8	theme	meso-diaminopimelic	1026:1044	arg1	type					970:973	The isomer type	959:973	The isomer type of diamino acid in the cell-wall peptidoglycan	959:1020	The isomer type of diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid.
30625108	3	9	theme	16S	324:326	arg1	sequences					338:346	16S rRNA gene sequences	324:346	16S rRNA gene sequences	324:346	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain K2E09-144T represents a novel member of the genus Cohnella within the family Paenibacillaceae of the phylum Firmicutes.
30625108	2	10	theme	Gram-stain-positive	75:93	arg1	isolate					156:162	A Gram-stain-positive, rod-shaped, endospore-forming, motile and aerobic bacterial isolate	73:162	A Gram-stain-positive, rod-shaped, endospore-forming, motile and aerobic bacterial isolate	73:162	A Gram-stain-positive, rod-shaped, endospore-forming, motile and aerobic bacterial isolate, designated strain K2E09-144T, was obtained from animal faeces that were collected from a karst cave in Guizhou province, China.
30625108	5	11	theme	fatty	638:642	arg1	anteiso-C15:0					655:667	anteiso-C15:0	655:667	anteiso-C15:0	655:667	The major cellular fatty acids were anteiso-C15:0, iso-C16:0 and C16 : 0.
30625108	5	11	theme	fatty	638:642	arg1	acids					644:648	The major cellular fatty acids	619:648	The major cellular fatty acids	619:648	The major cellular fatty acids were anteiso-C15:0, iso-C16:0 and C16 : 0.
30625108	9	12	theme	strain	1066:1071	arg1	K2E09-144T					1073:1082	strain K2E09-144T	1066:1082	strain K2E09-144T	1066:1082	The genome of strain K2E09-144T comprised 6.43 Mb, and encoded 6029 genes in total.
30625108	4	13	theme	rRNA	580:583	arg1	similarity					599:608	16S rRNA gene sequence similarity	576:608	16S rRNA gene sequence similarity 97.0 %	576:615	Strain K2E09-144T was phylogenetically closely related to Cohnella nanjingensis D45T (16S rRNA gene sequence similarity 97.0 %).
30625108	3	14	theme	rRNA	328:331	arg1	sequences					338:346	16S rRNA gene sequences	324:346	16S rRNA gene sequences	324:346	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain K2E09-144T represents a novel member of the genus Cohnella within the family Paenibacillaceae of the phylum Firmicutes.
30625108	11	15	theme	strain	1264:1269	arg1	K2E09-144T					1271:1280	strain K2E09-144T	1264:1280	strain K2E09-144T	1264:1280	Based on its phylogenetic, phenotypic and chemotaxonomic characteristics, strain K2E09-144T is considered to represent a novel species of the genus Cohnella, for which the name Cohnella faecalis sp.
30625108	8	16	theme	acid	986:989	arg1	acid					1046:1049	meso-diaminopimelic acid	1026:1049	meso-diaminopimelic acid	1026:1049	The isomer type of diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid.
30625108	8	16	theme	acid	986:989	arg1	type					970:973	The isomer type	959:973	The isomer type of diamino acid in the cell-wall peptidoglycan	959:1020	The isomer type of diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid.
30625108	2	17	theme	karst	254:258	arg1	cave					260:263	a karst cave	252:263	a karst cave in Guizhou province, China	252:290	A Gram-stain-positive, rod-shaped, endospore-forming, motile and aerobic bacterial isolate, designated strain K2E09-144T, was obtained from animal faeces that were collected from a karst cave in Guizhou province, China.
30625108	9	18	theme	K2E09-144T	1073:1082	arg1	genome					1056:1061	The genome	1052:1061	The genome of strain K2E09-144T	1052:1082	The genome of strain K2E09-144T comprised 6.43 Mb, and encoded 6029 genes in total.
30625108	9	18	theme	K2E09-144T	1073:1082	arg1	genes					1120:1124	6029 genes	1115:1124	6029 genes in total	1115:1133	The genome of strain K2E09-144T comprised 6.43 Mb, and encoded 6029 genes in total.
30625108	1	19	attach	isolated	28:35	arg1	animal					42:47	animal	42:47	animal	42:47	nov., isolated from animal faeces in a karst cave.
30625108	1	19	attach	isolated	28:35	arg2	nov.					22:25	nov.	22:25	nov.	22:25	nov., isolated from animal faeces in a karst cave.
30625108	2	20	theme	motile	127:132	arg1	isolate					156:162	A Gram-stain-positive, rod-shaped, endospore-forming, motile and aerobic bacterial isolate	73:162	A Gram-stain-positive, rod-shaped, endospore-forming, motile and aerobic bacterial isolate	73:162	A Gram-stain-positive, rod-shaped, endospore-forming, motile and aerobic bacterial isolate, designated strain K2E09-144T, was obtained from animal faeces that were collected from a karst cave in Guizhou province, China.
30625108	8	21	theme	diamino	978:984	arg1	acid					986:989	diamino acid	978:989	diamino acid	978:989	The isomer type of diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid.
30625108	7	22	theme	unidentified	883:894	arg1	aminophospholipids					896:913	four unidentified aminophospholipids	878:913	four unidentified aminophospholipids	878:913	The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified phospholipid, four unidentified aminophospholipids, one glycolipid and one unidentified lipid.
30625108	11	23	theme	Cohnella	1338:1345	arg1	species					1317:1323	a novel species	1309:1323	a novel species	1309:1323	Based on its phylogenetic, phenotypic and chemotaxonomic characteristics, strain K2E09-144T is considered to represent a novel species of the genus Cohnella, for which the name Cohnella faecalis sp.
30625108	4	24	theme	16S	576:578	arg1	similarity					599:608	16S rRNA gene sequence similarity	576:608	16S rRNA gene sequence similarity 97.0 %	576:615	Strain K2E09-144T was phylogenetically closely related to Cohnella nanjingensis D45T (16S rRNA gene sequence similarity 97.0 %).
30625108	10	25	theme	DNA	1171:1173	arg1	%					1187:1187	53.3 mol%	1179:1187	53.3 mol%	1179:1187	The DNA G+C content of the genomic DNA was 53.3 mol%.
30625108	10	25	theme	DNA	1171:1173	arg1	content					1148:1154	The DNA G+C content	1136:1154	The DNA G+C content of the genomic DNA	1136:1173	The DNA G+C content of the genomic DNA was 53.3 mol%.
30625108	13	26	theme	type	1411:1414	arg1	strain					1416:1421	The type strain	1407:1421	The type strain	1407:1421	The type strain is K2E09-144T (=CGMCC 1.13587T=NBRC 113454T).
30625108	13	26	theme	type	1411:1414	arg1	K2E09-144T					1426:1435	K2E09-144T	1426:1435	K2E09-144T (=CGMCC 1.13587T=NBRC 113454T)	1426:1466	The type strain is K2E09-144T (=CGMCC 1.13587T=NBRC 113454T).
30625108	0	27	theme	faecalis	9:16	arg1	sp					18:19	Cohnella faecalis sp	0:19	Cohnella faecalis sp.	0:20	Cohnella faecalis sp.
30625108	2	28	theme	endospore-forming	108:124	arg1	isolate					156:162	A Gram-stain-positive, rod-shaped, endospore-forming, motile and aerobic bacterial isolate	73:162	A Gram-stain-positive, rod-shaped, endospore-forming, motile and aerobic bacterial isolate	73:162	A Gram-stain-positive, rod-shaped, endospore-forming, motile and aerobic bacterial isolate, designated strain K2E09-144T, was obtained from animal faeces that were collected from a karst cave in Guizhou province, China.
30625108	7	29	theme	unidentified	939:950	arg1	lipid					952:956	one unidentified lipid	935:956	one unidentified lipid	935:956	The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified phospholipid, four unidentified aminophospholipids, one glycolipid and one unidentified lipid.
30625108	1	30	theme	karst	61:65	arg1	cave					67:70	a karst cave	59:70	a karst cave	59:70	nov., isolated from animal faeces in a karst cave.
30625108	3	31	theme	novel	394:398	arg1	member					400:405	a novel member	392:405	a novel member of the genus Cohnella	392:427	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain K2E09-144T represents a novel member of the genus Cohnella within the family Paenibacillaceae of the phylum Firmicutes.
30625108	0	32	theme	Cohnella	0:7	arg1	sp					18:19	Cohnella faecalis sp	0:19	Cohnella faecalis sp.	0:20	Cohnella faecalis sp.
30625108	7	33	theme	polar	757:761	arg1	phosphatidylethanolamine					775:798	phosphatidylethanolamine	775:798	phosphatidylethanolamine	775:798	The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified phospholipid, four unidentified aminophospholipids, one glycolipid and one unidentified lipid.
30625108	7	33	theme	polar	757:761	arg1	lipids					763:768	The major polar lipids	747:768	The major polar lipids	747:768	The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified phospholipid, four unidentified aminophospholipids, one glycolipid and one unidentified lipid.
30625108	11	34	theme	Cohnella	1367:1374	arg1	sp					1385:1386	the name Cohnella faecalis sp	1358:1386	the name Cohnella faecalis sp	1358:1386	Based on its phylogenetic, phenotypic and chemotaxonomic characteristics, strain K2E09-144T is considered to represent a novel species of the genus Cohnella, for which the name Cohnella faecalis sp.
30625108	3	35	theme	gene	333:336	arg1	sequences					338:346	16S rRNA gene sequences	324:346	16S rRNA gene sequences	324:346	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain K2E09-144T represents a novel member of the genus Cohnella within the family Paenibacillaceae of the phylum Firmicutes.
30625108	11	36	theme	phylogenetic	1203:1214	arg1	characteristics					1247:1261	its phylogenetic, phenotypic and chemotaxonomic characteristics	1199:1261	its phylogenetic, phenotypic and chemotaxonomic characteristics	1199:1261	Based on its phylogenetic, phenotypic and chemotaxonomic characteristics, strain K2E09-144T is considered to represent a novel species of the genus Cohnella, for which the name Cohnella faecalis sp.
30625108	8	37	theme	cell-wall	998:1006	arg1	peptidoglycan					1008:1020	the cell-wall peptidoglycan	994:1020	the cell-wall peptidoglycan	994:1020	The isomer type of diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid.
30625108	10	38	theme	genomic	1163:1169	arg1	DNA					1171:1173	the genomic DNA	1159:1173	the genomic DNA	1159:1173	The DNA G+C content of the genomic DNA was 53.3 mol%.
30625108	11	39	theme	novel	1311:1315	arg1	species					1317:1323	a novel species	1309:1323	a novel species	1309:1323	Based on its phylogenetic, phenotypic and chemotaxonomic characteristics, strain K2E09-144T is considered to represent a novel species of the genus Cohnella, for which the name Cohnella faecalis sp.
30625108	10	40	theme	53.3 mol	1179:1186	arg1	%					1187:1187	53.3 mol%	1179:1187	53.3 mol%	1179:1187	The DNA G+C content of the genomic DNA was 53.3 mol%.
30625108	10	40	theme	53.3 mol	1179:1186	arg1	content					1148:1154	The DNA G+C content	1136:1154	The DNA G+C content of the genomic DNA	1136:1173	The DNA G+C content of the genomic DNA was 53.3 mol%.
30625108	9	41	theme	6029	1115:1118	arg1	genome					1056:1061	The genome	1052:1061	The genome of strain K2E09-144T	1052:1082	The genome of strain K2E09-144T comprised 6.43 Mb, and encoded 6029 genes in total.
30625108	9	41	theme	6029	1115:1118	arg1	genes					1120:1124	6029 genes	1115:1124	6029 genes in total	1115:1133	The genome of strain K2E09-144T comprised 6.43 Mb, and encoded 6029 genes in total.
30625108	4	42	theme	%	615:615	arg1	similarity					599:608	16S rRNA gene sequence similarity	576:608	16S rRNA gene sequence similarity 97.0 %	576:615	Strain K2E09-144T was phylogenetically closely related to Cohnella nanjingensis D45T (16S rRNA gene sequence similarity 97.0 %).
30625108	7	43	theme	major	751:755	arg1	phosphatidylethanolamine					775:798	phosphatidylethanolamine	775:798	phosphatidylethanolamine	775:798	The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified phospholipid, four unidentified aminophospholipids, one glycolipid and one unidentified lipid.
30625108	7	43	theme	major	751:755	arg1	lipids					763:768	The major polar lipids	747:768	The major polar lipids	747:768	The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified phospholipid, four unidentified aminophospholipids, one glycolipid and one unidentified lipid.
30625108	13	44	theme	1.13587T=NBRC	1445:1457	arg1	113454T					1459:1465	=CGMCC 1.13587T=NBRC 113454T	1438:1465	=CGMCC 1.13587T=NBRC 113454T	1438:1465	The type strain is K2E09-144T (=CGMCC 1.13587T=NBRC 113454T).
30625108	13	44	theme	1.13587T=NBRC	1445:1457	arg1	K2E09-144T					1426:1435	K2E09-144T	1426:1435	K2E09-144T (=CGMCC 1.13587T=NBRC 113454T)	1426:1466	The type strain is K2E09-144T (=CGMCC 1.13587T=NBRC 113454T).
30625108	2	45	theme	animal	213:218	arg1	faeces					220:225	animal faeces	213:225	animal faeces that were collected from a karst cave in Guizhou province, China	213:290	A Gram-stain-positive, rod-shaped, endospore-forming, motile and aerobic bacterial isolate, designated strain K2E09-144T, was obtained from animal faeces that were collected from a karst cave in Guizhou province, China.
30625108	3	46	theme	genus	414:418	arg1	Cohnella					420:427	the genus Cohnella	410:427	the genus Cohnella	410:427	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain K2E09-144T represents a novel member of the genus Cohnella within the family Paenibacillaceae of the phylum Firmicutes.
30625108	11	47	dep	Cohnella	1367:1374	arg1	faecalis					1376:1383	faecalis	1376:1383	faecalis	1376:1383	Based on its phylogenetic, phenotypic and chemotaxonomic characteristics, strain K2E09-144T is considered to represent a novel species of the genus Cohnella, for which the name Cohnella faecalis sp.
30625108	2	48	theme	bacterial	146:154	arg1	isolate					156:162	A Gram-stain-positive, rod-shaped, endospore-forming, motile and aerobic bacterial isolate	73:162	A Gram-stain-positive, rod-shaped, endospore-forming, motile and aerobic bacterial isolate	73:162	A Gram-stain-positive, rod-shaped, endospore-forming, motile and aerobic bacterial isolate, designated strain K2E09-144T, was obtained from animal faeces that were collected from a karst cave in Guizhou province, China.
30625108	9	49	from	genes	1120:1124	arg1	total					1129:1133	total	1129:1133	total	1129:1133	The genome of strain K2E09-144T comprised 6.43 Mb, and encoded 6029 genes in total.
30625108	3	50	theme	Cohnella	420:427	arg1	member					400:405	a novel member	392:405	a novel member of the genus Cohnella	392:427	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain K2E09-144T represents a novel member of the genus Cohnella within the family Paenibacillaceae of the phylum Firmicutes.
30625108	3	51	theme	strain	363:368	arg1	K2E09-144T					370:379	strain K2E09-144T	363:379	strain K2E09-144T	363:379	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain K2E09-144T represents a novel member of the genus Cohnella within the family Paenibacillaceae of the phylum Firmicutes.
30625108	11	52	theme	chemotaxonomic	1232:1245	arg1	characteristics					1247:1261	its phylogenetic, phenotypic and chemotaxonomic characteristics	1199:1261	its phylogenetic, phenotypic and chemotaxonomic characteristics	1199:1261	Based on its phylogenetic, phenotypic and chemotaxonomic characteristics, strain K2E09-144T is considered to represent a novel species of the genus Cohnella, for which the name Cohnella faecalis sp.
30625108	2	53	theme	aerobic	138:144	arg1	isolate					156:162	A Gram-stain-positive, rod-shaped, endospore-forming, motile and aerobic bacterial isolate	73:162	A Gram-stain-positive, rod-shaped, endospore-forming, motile and aerobic bacterial isolate	73:162	A Gram-stain-positive, rod-shaped, endospore-forming, motile and aerobic bacterial isolate, designated strain K2E09-144T, was obtained from animal faeces that were collected from a karst cave in Guizhou province, China.
30625108	13	54	theme	=CGMCC	1438:1443	arg1	113454T					1459:1465	=CGMCC 1.13587T=NBRC 113454T	1438:1465	=CGMCC 1.13587T=NBRC 113454T	1438:1465	The type strain is K2E09-144T (=CGMCC 1.13587T=NBRC 113454T).
30625108	13	54	theme	=CGMCC	1438:1443	arg1	K2E09-144T					1426:1435	K2E09-144T	1426:1435	K2E09-144T (=CGMCC 1.13587T=NBRC 113454T)	1426:1466	The type strain is K2E09-144T (=CGMCC 1.13587T=NBRC 113454T).
30625108	11	55	theme	name	1362:1365	arg1	sp					1385:1386	the name Cohnella faecalis sp	1358:1386	the name Cohnella faecalis sp	1358:1386	Based on its phylogenetic, phenotypic and chemotaxonomic characteristics, strain K2E09-144T is considered to represent a novel species of the genus Cohnella, for which the name Cohnella faecalis sp.
30625108	10	56	theme	G+C	1144:1146	arg1	%					1187:1187	53.3 mol%	1179:1187	53.3 mol%	1179:1187	The DNA G+C content of the genomic DNA was 53.3 mol%.
30625108	10	56	theme	G+C	1144:1146	arg1	content					1148:1154	The DNA G+C content	1136:1154	The DNA G+C content of the genomic DNA	1136:1173	The DNA G+C content of the genomic DNA was 53.3 mol%.
30625108	10	57	theme	DNA	1140:1142	arg1	%					1187:1187	53.3 mol%	1179:1187	53.3 mol%	1179:1187	The DNA G+C content of the genomic DNA was 53.3 mol%.
30625108	10	57	theme	DNA	1140:1142	arg1	content					1148:1154	The DNA G+C content	1136:1154	The DNA G+C content of the genomic DNA	1136:1173	The DNA G+C content of the genomic DNA was 53.3 mol%.
30625108	11	58	theme	phenotypic	1217:1226	arg1	characteristics					1247:1261	its phylogenetic, phenotypic and chemotaxonomic characteristics	1199:1261	its phylogenetic, phenotypic and chemotaxonomic characteristics	1199:1261	Based on its phylogenetic, phenotypic and chemotaxonomic characteristics, strain K2E09-144T is considered to represent a novel species of the genus Cohnella, for which the name Cohnella faecalis sp.
30625108	3	59	theme	family	440:445	arg1	Paenibacillaceae					447:462	the family Paenibacillaceae	436:462	the family Paenibacillaceae of the phylum Firmicutes	436:487	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain K2E09-144T represents a novel member of the genus Cohnella within the family Paenibacillaceae of the phylum Firmicutes.
30625108	4	60	theme	sequence	590:597	arg1	similarity					599:608	16S rRNA gene sequence similarity	576:608	16S rRNA gene sequence similarity 97.0 %	576:615	Strain K2E09-144T was phylogenetically closely related to Cohnella nanjingensis D45T (16S rRNA gene sequence similarity 97.0 %).
30625108	2	61	theme	strain	176:181	arg1	K2E09-144T					183:192	strain K2E09-144T	176:192	strain K2E09-144T	176:192	A Gram-stain-positive, rod-shaped, endospore-forming, motile and aerobic bacterial isolate, designated strain K2E09-144T, was obtained from animal faeces that were collected from a karst cave in Guizhou province, China.
30625108	6	62	theme	isoprenoid	703:712	arg1	menaqinone					726:735	menaqinone 7	726:737	menaqinone 7 (MK-7)	726:744	The major isoprenoid quinone was menaqinone 7 (MK-7).
30625108	6	62	theme	isoprenoid	703:712	arg1	quinone					714:720	The major isoprenoid quinone	693:720	The major isoprenoid quinone	693:720	The major isoprenoid quinone was menaqinone 7 (MK-7).
30625108	8	63	from	type	970:973	arg1	peptidoglycan					1008:1020	the cell-wall peptidoglycan	994:1020	the cell-wall peptidoglycan	994:1020	The isomer type of diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid.
30625108	2	64	from	cave	260:263	arg1	China					286:290	China	286:290	China	286:290	A Gram-stain-positive, rod-shaped, endospore-forming, motile and aerobic bacterial isolate, designated strain K2E09-144T, was obtained from animal faeces that were collected from a karst cave in Guizhou province, China.
30625108	4	65	theme	gene	585:588	arg1	similarity					599:608	16S rRNA gene sequence similarity	576:608	16S rRNA gene sequence similarity 97.0 %	576:615	Strain K2E09-144T was phylogenetically closely related to Cohnella nanjingensis D45T (16S rRNA gene sequence similarity 97.0 %).
30625108	3	66	theme	Phylogenetic	293:304	arg1	analysis					306:313	Phylogenetic analysis	293:313	Phylogenetic analysis based on 16S rRNA gene sequences	293:346	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain K2E09-144T represents a novel member of the genus Cohnella within the family Paenibacillaceae of the phylum Firmicutes.
30625108	6	67	theme	major	697:701	arg1	menaqinone					726:735	menaqinone 7	726:737	menaqinone 7 (MK-7)	726:744	The major isoprenoid quinone was menaqinone 7 (MK-7).
30625108	6	67	theme	major	697:701	arg1	quinone					714:720	The major isoprenoid quinone	693:720	The major isoprenoid quinone	693:720	The major isoprenoid quinone was menaqinone 7 (MK-7).
30625108	4	68	theme	97.0 	610:614	arg1	%					615:615	%	615:615	%	615:615	Strain K2E09-144T was phylogenetically closely related to Cohnella nanjingensis D45T (16S rRNA gene sequence similarity 97.0 %).
30625108	11	69	theme	genus	1332:1336	arg1	Cohnella					1338:1345	the genus Cohnella	1328:1345	the genus Cohnella	1328:1345	Based on its phylogenetic, phenotypic and chemotaxonomic characteristics, strain K2E09-144T is considered to represent a novel species of the genus Cohnella, for which the name Cohnella faecalis sp.
30625108	8	70	theme	isomer	963:968	arg1	acid					1046:1049	meso-diaminopimelic acid	1026:1049	meso-diaminopimelic acid	1026:1049	The isomer type of diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid.
30625108	8	70	theme	isomer	963:968	arg1	type					970:973	The isomer type	959:973	The isomer type of diamino acid in the cell-wall peptidoglycan	959:1020	The isomer type of diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid.
30551956	8	0	theme	fructose-6-phosphate	1035:1054	arg1	enzyme					1072:1077	the fructose-6-phosphate phosphoketolase enzyme	1031:1077	the fructose-6-phosphate phosphoketolase enzyme	1031:1077	Isolated strains are Gram-positive, lactate- and acetate-producing and possess the fructose-6-phosphate phosphoketolase enzyme.
30551956	5	1	theme	solitary	644:651	arg1	bees					653:656	solitary bees	644:656	solitary bees	644:656	To date there is sparse information about bifidobacteria in solitary bees such as Xylocopa and Osmia spp., this study is therefore focused on the isolation and characterization of bifidobacterial strains from solitary bees, in particular carpenter bee (Xylocopa violacea), builder bee (Osmia cornuta), and red mason bee (Osmia rufa).
30551956	2	2	theme	Xylocopa	100:107	arg1	tract					129:133	the carpenter bee (Xylocopa violacea) digestive tract	81:133	the carpenter bee (Xylocopa violacea) digestive tract	81:133	nov., from the carpenter bee (Xylocopa violacea) digestive tract.
30551956	5	3	theme	bifidobacterial	615:629	arg1	strains					631:637	bifidobacterial strains	615:637	bifidobacterial strains from solitary bees, in particular carpenter bee (Xylocopa violacea)	615:705	To date there is sparse information about bifidobacteria in solitary bees such as Xylocopa and Osmia spp., this study is therefore focused on the isolation and characterization of bifidobacterial strains from solitary bees, in particular carpenter bee (Xylocopa violacea), builder bee (Osmia cornuta), and red mason bee (Osmia rufa).
30551956	9	4	theme	genome	1085:1090	arg1	sequencing					1092:1101	Full genome sequencing	1080:1101	Full genome sequencing	1080:1101	Full genome sequencing and genome annotation were performed for XV2 and XV10.
30551956	6	5	from	Osmia	793:797	arg1	isolates					779:786	the isolates	775:786	the isolates from Osmia	775:797	Among the isolates from Osmia spp.
30551956	10	6	theme	new	1314:1316	arg1	strains					1318:1324	the new strains	1310:1324	the new strains	1310:1324	Phylogenetic relationships were determined using partial and complete 16S rRNA sequences and hsp60 restriction analysis that confirmed the belonging of the new strains to Bifidobacterium genus and the relatedness of the strains XV2 and XV10 with XV16 and XV4, respectively.
30551956	5	7	theme	sparse	452:457	arg1	information					459:469	sparse information	452:469	sparse information about bifidobacteria in solitary bees such as Xylocopa	452:524	To date there is sparse information about bifidobacteria in solitary bees such as Xylocopa and Osmia spp., this study is therefore focused on the isolation and characterization of bifidobacterial strains from solitary bees, in particular carpenter bee (Xylocopa violacea), builder bee (Osmia cornuta), and red mason bee (Osmia rufa).
30551956	3	8	theme	important	233:241	arg1	niche					243:247	an important niche	230:247	an important niche	230:247	Social bees harbor a community of gut mutualistic bacteria, among which bifidobacteria occupy an important niche.
30551956	4	9	theme	bifidobacterial	370:384	arg1	present					404:410	present	404:410	present	404:410	Recently, four novel species have been isolated from guts of different bumblebees, thus allowing to suppose that a core bifidobacterial population may be present in wild solitary bees.
30551956	4	9	theme	bifidobacterial	370:384	arg1	population					386:395	a core bifidobacterial population	363:395	a core bifidobacterial population	363:395	Recently, four novel species have been isolated from guts of different bumblebees, thus allowing to suppose that a core bifidobacterial population may be present in wild solitary bees.
30551956	0	10	theme	Bifidobacterium	0:14	arg1	sp					26:27	Bifidobacterium xylocopae sp	0:27	Bifidobacterium xylocopae sp.	0:28	Bifidobacterium xylocopae sp.
30551956	4	11	from	present	404:410	arg1	bees					429:432	wild solitary bees	415:432	wild solitary bees	415:432	Recently, four novel species have been isolated from guts of different bumblebees, thus allowing to suppose that a core bifidobacterial population may be present in wild solitary bees.
30551956	11	12	theme	phylogenetic	1547:1558	arg1	tree					1560:1563	the phylogenetic tree	1543:1563	the phylogenetic tree	1543:1563	Phenotypic tests were performed for the proposed type strains, reference strains and their closest neighbor in the phylogenetic tree.
30551956	11	13	theme	proposed	1472:1479	arg1	strains					1486:1492	the proposed type strains	1468:1492	the proposed type strains	1468:1492	Phenotypic tests were performed for the proposed type strains, reference strains and their closest neighbor in the phylogenetic tree.
30551956	14	14	theme	104956T=LMG	1801:1811	arg1	30143T					1813:1818	=DSM 104956T=LMG 30143T	1796:1818	=DSM 104956T=LMG 30143T	1796:1818	nov. whose type strain is XV10 (=DSM 104956T=LMG 30143T), reference strain XV4.
30551956	14	14	theme	104956T=LMG	1801:1811	arg1	XV10					1790:1793	XV10	1790:1793	XV10 (=DSM 104956T=LMG 30143T)	1790:1819	nov. whose type strain is XV10 (=DSM 104956T=LMG 30143T), reference strain XV4.
30551956	13	15	theme	=DSM	1681:1684	arg1	XV2					1676:1678	XV2	1676:1678	XV2 (=DSM 104955T=LMG 30142T)	1676:1704	nov. whose type strain is XV2 (=DSM 104955T=LMG 30142T), reference strain XV16 and Bifidobacterium aemilianum sp.
30551956	13	15	theme	=DSM	1681:1684	arg1	30142T					1698:1703	=DSM 104955T=LMG 30142T	1681:1703	=DSM 104955T=LMG 30142T	1681:1703	nov. whose type strain is XV2 (=DSM 104955T=LMG 30142T), reference strain XV16 and Bifidobacterium aemilianum sp.
30551956	5	16	theme	carpenter	673:681	arg1	bee					683:685	particular carpenter bee	662:685	particular carpenter bee (Xylocopa violacea)	662:705	To date there is sparse information about bifidobacteria in solitary bees such as Xylocopa and Osmia spp., this study is therefore focused on the isolation and characterization of bifidobacterial strains from solitary bees, in particular carpenter bee (Xylocopa violacea), builder bee (Osmia cornuta), and red mason bee (Osmia rufa).
30551956	5	16	theme	carpenter	673:681	arg1	violacea					697:704	Xylocopa violacea	688:704	Xylocopa violacea	688:704	To date there is sparse information about bifidobacteria in solitary bees such as Xylocopa and Osmia spp., this study is therefore focused on the isolation and characterization of bifidobacterial strains from solitary bees, in particular carpenter bee (Xylocopa violacea), builder bee (Osmia cornuta), and red mason bee (Osmia rufa).
30551956	8	17	contain	possess	1023:1029	arg2	enzyme					1072:1077	the fructose-6-phosphate phosphoketolase enzyme	1031:1077	the fructose-6-phosphate phosphoketolase enzyme	1031:1077	Isolated strains are Gram-positive, lactate- and acetate-producing and possess the fructose-6-phosphate phosphoketolase enzyme.
30551956	8	17	contain	possess	1023:1029	arg1	strains					961:967	Isolated strains	952:967	Isolated strains	952:967	Isolated strains are Gram-positive, lactate- and acetate-producing and possess the fructose-6-phosphate phosphoketolase enzyme.
30551956	2	18	from	tract	129:133	arg1	nov.					70:73	nov.	70:73	nov.	70:73	nov., from the carpenter bee (Xylocopa violacea) digestive tract.
30551956	4	19	theme	wild	415:418	arg1	bees					429:432	wild solitary bees	415:432	wild solitary bees	415:432	Recently, four novel species have been isolated from guts of different bumblebees, thus allowing to suppose that a core bifidobacterial population may be present in wild solitary bees.
30551956	4	20	located	present	404:410	arg2	population					386:395	a core bifidobacterial population	363:395	a core bifidobacterial population	363:395	Recently, four novel species have been isolated from guts of different bumblebees, thus allowing to suppose that a core bifidobacterial population may be present in wild solitary bees.
30551956	4	20	located	present	404:410	arg1	bees					429:432	wild solitary bees	415:432	wild solitary bees	415:432	Recently, four novel species have been isolated from guts of different bumblebees, thus allowing to suppose that a core bifidobacterial population may be present in wild solitary bees.
30551956	4	20	located	present	404:410	arg2	present					404:410	present	404:410	present	404:410	Recently, four novel species have been isolated from guts of different bumblebees, thus allowing to suppose that a core bifidobacterial population may be present in wild solitary bees.
30551956	2	21	theme	bee	95:97	arg1	tract					129:133	the carpenter bee (Xylocopa violacea) digestive tract	81:133	the carpenter bee (Xylocopa violacea) digestive tract	81:133	nov., from the carpenter bee (Xylocopa violacea) digestive tract.
30551956	7	22	theme	new	928:930	arg1	species					932:938	putative new species	919:938	putative new species	919:938	no new species have been detected whereas among Xylocopa isolates four strains (XV2, XV4, XV10, XV16) belonging to putative new species were found.
30551956	14	23	theme	type	1775:1778	arg1	strain					1780:1785	strain	1780:1785	strain	1780:1785	nov. whose type strain is XV10 (=DSM 104956T=LMG 30143T), reference strain XV4.
30551956	12	24	theme	xylocopae	1636:1644	arg1	sp					1646:1647	two novel species Bifidobacterium xylocopae sp	1602:1647	two novel species Bifidobacterium xylocopae sp	1602:1647	The results support the proposal of two novel species Bifidobacterium xylocopae sp.
30551956	1	25	dep	sp	66:67	arg1	nov.					30:33	nov.	30:33	nov.	30:33	nov. and Bifidobacterium aemilianum sp.
30551956	1	25	dep	sp	66:67	arg1	aemilianum					55:64	Bifidobacterium aemilianum	39:64	Bifidobacterium aemilianum	39:64	nov. and Bifidobacterium aemilianum sp.
30551956	3	26	theme	Social	136:141	arg1	bees					143:146	Social bees	136:146	Social bees	136:146	Social bees harbor a community of gut mutualistic bacteria, among which bifidobacteria occupy an important niche.
30551956	10	27	theme	strains	1378:1384	arg1	genus					1345:1349	Bifidobacterium genus	1329:1349	Bifidobacterium genus	1329:1349	Phylogenetic relationships were determined using partial and complete 16S rRNA sequences and hsp60 restriction analysis that confirmed the belonging of the new strains to Bifidobacterium genus and the relatedness of the strains XV2 and XV10 with XV16 and XV4, respectively.
30551956	10	27	theme	strains	1378:1384	arg1	relatedness					1359:1369	the relatedness	1355:1369	the relatedness of the strains XV2 and XV10	1355:1397	Phylogenetic relationships were determined using partial and complete 16S rRNA sequences and hsp60 restriction analysis that confirmed the belonging of the new strains to Bifidobacterium genus and the relatedness of the strains XV2 and XV10 with XV16 and XV4, respectively.
30551956	5	28	theme	Osmia	721:725	arg1	bee					716:718	builder bee	708:718	builder bee (Osmia cornuta)	708:734	To date there is sparse information about bifidobacteria in solitary bees such as Xylocopa and Osmia spp., this study is therefore focused on the isolation and characterization of bifidobacterial strains from solitary bees, in particular carpenter bee (Xylocopa violacea), builder bee (Osmia cornuta), and red mason bee (Osmia rufa).
30551956	5	28	theme	Osmia	721:725	arg1	cornuta					727:733	Osmia cornuta	721:733	Osmia cornuta	721:733	To date there is sparse information about bifidobacteria in solitary bees such as Xylocopa and Osmia spp., this study is therefore focused on the isolation and characterization of bifidobacterial strains from solitary bees, in particular carpenter bee (Xylocopa violacea), builder bee (Osmia cornuta), and red mason bee (Osmia rufa).
30551956	12	29	theme	species	1612:1618	arg1	sp					1646:1647	two novel species Bifidobacterium xylocopae sp	1602:1647	two novel species Bifidobacterium xylocopae sp	1602:1647	The results support the proposal of two novel species Bifidobacterium xylocopae sp.
30551956	4	30	attach	isolated	289:296	arg1	guts					303:306	guts	303:306	guts of different bumblebees	303:330	Recently, four novel species have been isolated from guts of different bumblebees, thus allowing to suppose that a core bifidobacterial population may be present in wild solitary bees.
30551956	4	30	attach	isolated	289:296	arg2	species					271:277	four novel species	260:277	four novel species	260:277	Recently, four novel species have been isolated from guts of different bumblebees, thus allowing to suppose that a core bifidobacterial population may be present in wild solitary bees.
30551956	10	31	theme	Phylogenetic	1158:1169	arg1	relationships					1171:1183	Phylogenetic relationships	1158:1183	Phylogenetic relationships	1158:1183	Phylogenetic relationships were determined using partial and complete 16S rRNA sequences and hsp60 restriction analysis that confirmed the belonging of the new strains to Bifidobacterium genus and the relatedness of the strains XV2 and XV10 with XV16 and XV4, respectively.
30551956	5	32	from	bee	751:753	arg1	bees					653:656	solitary bees	644:656	solitary bees	644:656	To date there is sparse information about bifidobacteria in solitary bees such as Xylocopa and Osmia spp., this study is therefore focused on the isolation and characterization of bifidobacterial strains from solitary bees, in particular carpenter bee (Xylocopa violacea), builder bee (Osmia cornuta), and red mason bee (Osmia rufa).
30551956	3	33	theme	bacteria	186:193	arg1	community					157:165	a community	155:165	a community	155:165	Social bees harbor a community of gut mutualistic bacteria, among which bifidobacteria occupy an important niche.
30551956	5	34	theme	mason	745:749	arg1	bee					751:753	red mason bee	741:753	red mason bee (Osmia rufa)	741:766	To date there is sparse information about bifidobacteria in solitary bees such as Xylocopa and Osmia spp., this study is therefore focused on the isolation and characterization of bifidobacterial strains from solitary bees, in particular carpenter bee (Xylocopa violacea), builder bee (Osmia cornuta), and red mason bee (Osmia rufa).
30551956	5	34	theme	mason	745:749	arg1	rufa					762:765	Osmia rufa	756:765	Osmia rufa	756:765	To date there is sparse information about bifidobacteria in solitary bees such as Xylocopa and Osmia spp., this study is therefore focused on the isolation and characterization of bifidobacterial strains from solitary bees, in particular carpenter bee (Xylocopa violacea), builder bee (Osmia cornuta), and red mason bee (Osmia rufa).
30551956	3	35	theme	gut	170:172	arg1	bacteria					186:193	gut mutualistic bacteria	170:193	gut mutualistic bacteria	170:193	Social bees harbor a community of gut mutualistic bacteria, among which bifidobacteria occupy an important niche.
30551956	13	36	theme	aemilianum	1749:1758	arg1	sp					1760:1761	Bifidobacterium aemilianum sp	1733:1761	Bifidobacterium aemilianum sp	1733:1761	nov. whose type strain is XV2 (=DSM 104955T=LMG 30142T), reference strain XV16 and Bifidobacterium aemilianum sp.
30551956	8	37	theme	Isolated	952:959	arg1	strains					961:967	Isolated strains	952:967	Isolated strains	952:967	Isolated strains are Gram-positive, lactate- and acetate-producing and possess the fructose-6-phosphate phosphoketolase enzyme.
30551956	10	38	theme	16S	1228:1230	arg1	sequences					1237:1245	partial and complete 16S rRNA sequences	1207:1245	partial and complete 16S rRNA sequences	1207:1245	Phylogenetic relationships were determined using partial and complete 16S rRNA sequences and hsp60 restriction analysis that confirmed the belonging of the new strains to Bifidobacterium genus and the relatedness of the strains XV2 and XV10 with XV16 and XV4, respectively.
30551956	4	39	attach	present	404:410	arg2	population					386:395	a core bifidobacterial population	363:395	a core bifidobacterial population	363:395	Recently, four novel species have been isolated from guts of different bumblebees, thus allowing to suppose that a core bifidobacterial population may be present in wild solitary bees.
30551956	4	39	attach	present	404:410	arg1	bees					429:432	wild solitary bees	415:432	wild solitary bees	415:432	Recently, four novel species have been isolated from guts of different bumblebees, thus allowing to suppose that a core bifidobacterial population may be present in wild solitary bees.
30551956	4	39	attach	present	404:410	arg2	present					404:410	present	404:410	present	404:410	Recently, four novel species have been isolated from guts of different bumblebees, thus allowing to suppose that a core bifidobacterial population may be present in wild solitary bees.
30551956	4	40	theme	bumblebees	321:330	arg1	guts					303:306	guts	303:306	guts of different bumblebees	303:330	Recently, four novel species have been isolated from guts of different bumblebees, thus allowing to suppose that a core bifidobacterial population may be present in wild solitary bees.
30551956	14	41	theme	=DSM	1796:1799	arg1	30143T					1813:1818	=DSM 104956T=LMG 30143T	1796:1818	=DSM 104956T=LMG 30143T	1796:1818	nov. whose type strain is XV10 (=DSM 104956T=LMG 30143T), reference strain XV4.
30551956	14	41	theme	=DSM	1796:1799	arg1	XV10					1790:1793	XV10	1790:1793	XV10 (=DSM 104956T=LMG 30143T)	1790:1819	nov. whose type strain is XV10 (=DSM 104956T=LMG 30143T), reference strain XV4.
30551956	10	42	theme	hsp60	1251:1255	arg1	analysis					1269:1276	hsp60 restriction analysis	1251:1276	hsp60 restriction analysis	1251:1276	Phylogenetic relationships were determined using partial and complete 16S rRNA sequences and hsp60 restriction analysis that confirmed the belonging of the new strains to Bifidobacterium genus and the relatedness of the strains XV2 and XV10 with XV16 and XV4, respectively.
30551956	5	43	from	bifidobacteria	477:490	arg1	bees					504:507	solitary bees	495:507	solitary bees such as Xylocopa	495:524	To date there is sparse information about bifidobacteria in solitary bees such as Xylocopa and Osmia spp., this study is therefore focused on the isolation and characterization of bifidobacterial strains from solitary bees, in particular carpenter bee (Xylocopa violacea), builder bee (Osmia cornuta), and red mason bee (Osmia rufa).
30551956	5	43	from	bifidobacteria	477:490	arg1	Xylocopa					517:524	Xylocopa	517:524	Xylocopa	517:524	To date there is sparse information about bifidobacteria in solitary bees such as Xylocopa and Osmia spp., this study is therefore focused on the isolation and characterization of bifidobacterial strains from solitary bees, in particular carpenter bee (Xylocopa violacea), builder bee (Osmia cornuta), and red mason bee (Osmia rufa).
30551956	13	44	theme	reference	1707:1715	arg1	strain					1717:1722	reference strain XV16	1707:1727	reference strain XV16	1707:1727	nov. whose type strain is XV2 (=DSM 104955T=LMG 30142T), reference strain XV16 and Bifidobacterium aemilianum sp.
30551956	14	45	theme	reference	1822:1830	arg1	strain					1832:1837	reference strain XV4	1822:1841	reference strain XV4	1822:1841	nov. whose type strain is XV10 (=DSM 104956T=LMG 30143T), reference strain XV4.
30551956	14	45	theme	reference	1822:1830	arg1	XV10					1790:1793	XV10	1790:1793	XV10 (=DSM 104956T=LMG 30143T)	1790:1819	nov. whose type strain is XV10 (=DSM 104956T=LMG 30143T), reference strain XV4.
30551956	2	46	theme	violacea	109:116	arg1	tract					129:133	the carpenter bee (Xylocopa violacea) digestive tract	81:133	the carpenter bee (Xylocopa violacea) digestive tract	81:133	nov., from the carpenter bee (Xylocopa violacea) digestive tract.
30551956	9	47	theme	Full	1080:1083	arg1	sequencing					1092:1101	Full genome sequencing	1080:1101	Full genome sequencing	1080:1101	Full genome sequencing and genome annotation were performed for XV2 and XV10.
30551956	4	48	theme	core	365:368	arg1	present					404:410	present	404:410	present	404:410	Recently, four novel species have been isolated from guts of different bumblebees, thus allowing to suppose that a core bifidobacterial population may be present in wild solitary bees.
30551956	4	48	theme	core	365:368	arg1	population					386:395	a core bifidobacterial population	363:395	a core bifidobacterial population	363:395	Recently, four novel species have been isolated from guts of different bumblebees, thus allowing to suppose that a core bifidobacterial population may be present in wild solitary bees.
30551956	13	49	theme	104955T=LMG	1686:1696	arg1	XV2					1676:1678	XV2	1676:1678	XV2 (=DSM 104955T=LMG 30142T)	1676:1704	nov. whose type strain is XV2 (=DSM 104955T=LMG 30142T), reference strain XV16 and Bifidobacterium aemilianum sp.
30551956	13	49	theme	104955T=LMG	1686:1696	arg1	30142T					1698:1703	=DSM 104955T=LMG 30142T	1681:1703	=DSM 104955T=LMG 30142T	1681:1703	nov. whose type strain is XV2 (=DSM 104955T=LMG 30142T), reference strain XV16 and Bifidobacterium aemilianum sp.
30551956	5	50	theme	strains	631:637	arg1	isolation					581:589	the isolation	577:589	the isolation	577:589	To date there is sparse information about bifidobacteria in solitary bees such as Xylocopa and Osmia spp., this study is therefore focused on the isolation and characterization of bifidobacterial strains from solitary bees, in particular carpenter bee (Xylocopa violacea), builder bee (Osmia cornuta), and red mason bee (Osmia rufa).
30551956	5	50	theme	strains	631:637	arg1	bee					716:718	builder bee	708:718	builder bee (Osmia cornuta)	708:734	To date there is sparse information about bifidobacteria in solitary bees such as Xylocopa and Osmia spp., this study is therefore focused on the isolation and characterization of bifidobacterial strains from solitary bees, in particular carpenter bee (Xylocopa violacea), builder bee (Osmia cornuta), and red mason bee (Osmia rufa).
30551956	5	50	theme	strains	631:637	arg1	characterization					595:610	characterization	595:610	characterization	595:610	To date there is sparse information about bifidobacteria in solitary bees such as Xylocopa and Osmia spp., this study is therefore focused on the isolation and characterization of bifidobacterial strains from solitary bees, in particular carpenter bee (Xylocopa violacea), builder bee (Osmia cornuta), and red mason bee (Osmia rufa).
30551956	5	50	theme	strains	631:637	arg1	bee					751:753	red mason bee	741:753	red mason bee (Osmia rufa)	741:766	To date there is sparse information about bifidobacteria in solitary bees such as Xylocopa and Osmia spp., this study is therefore focused on the isolation and characterization of bifidobacterial strains from solitary bees, in particular carpenter bee (Xylocopa violacea), builder bee (Osmia cornuta), and red mason bee (Osmia rufa).
30551956	5	50	theme	strains	631:637	arg1	cornuta					727:733	Osmia cornuta	721:733	Osmia cornuta	721:733	To date there is sparse information about bifidobacteria in solitary bees such as Xylocopa and Osmia spp., this study is therefore focused on the isolation and characterization of bifidobacterial strains from solitary bees, in particular carpenter bee (Xylocopa violacea), builder bee (Osmia cornuta), and red mason bee (Osmia rufa).
30551956	5	50	theme	strains	631:637	arg1	rufa					762:765	Osmia rufa	756:765	Osmia rufa	756:765	To date there is sparse information about bifidobacteria in solitary bees such as Xylocopa and Osmia spp., this study is therefore focused on the isolation and characterization of bifidobacterial strains from solitary bees, in particular carpenter bee (Xylocopa violacea), builder bee (Osmia cornuta), and red mason bee (Osmia rufa).
30551956	8	51	theme	phosphoketolase	1056:1070	arg1	enzyme					1072:1077	the fructose-6-phosphate phosphoketolase enzyme	1031:1077	the fructose-6-phosphate phosphoketolase enzyme	1031:1077	Isolated strains are Gram-positive, lactate- and acetate-producing and possess the fructose-6-phosphate phosphoketolase enzyme.
30551956	9	52	theme	genome	1107:1112	arg1	annotation					1114:1123	genome annotation	1107:1123	genome annotation	1107:1123	Full genome sequencing and genome annotation were performed for XV2 and XV10.
30551956	2	53	theme	digestive	119:127	arg1	tract					129:133	the carpenter bee (Xylocopa violacea) digestive tract	81:133	the carpenter bee (Xylocopa violacea) digestive tract	81:133	nov., from the carpenter bee (Xylocopa violacea) digestive tract.
30551956	5	54	theme	particular	662:671	arg1	bee					683:685	particular carpenter bee	662:685	particular carpenter bee (Xylocopa violacea)	662:705	To date there is sparse information about bifidobacteria in solitary bees such as Xylocopa and Osmia spp., this study is therefore focused on the isolation and characterization of bifidobacterial strains from solitary bees, in particular carpenter bee (Xylocopa violacea), builder bee (Osmia cornuta), and red mason bee (Osmia rufa).
30551956	5	54	theme	particular	662:671	arg1	violacea					697:704	Xylocopa violacea	688:704	Xylocopa violacea	688:704	To date there is sparse information about bifidobacteria in solitary bees such as Xylocopa and Osmia spp., this study is therefore focused on the isolation and characterization of bifidobacterial strains from solitary bees, in particular carpenter bee (Xylocopa violacea), builder bee (Osmia cornuta), and red mason bee (Osmia rufa).
30551956	10	55	theme	Bifidobacterium	1329:1343	arg1	genus					1345:1349	Bifidobacterium genus	1329:1349	Bifidobacterium genus	1329:1349	Phylogenetic relationships were determined using partial and complete 16S rRNA sequences and hsp60 restriction analysis that confirmed the belonging of the new strains to Bifidobacterium genus and the relatedness of the strains XV2 and XV10 with XV16 and XV4, respectively.
30551956	5	56	theme	Osmia	756:760	arg1	bee					751:753	red mason bee	741:753	red mason bee (Osmia rufa)	741:766	To date there is sparse information about bifidobacteria in solitary bees such as Xylocopa and Osmia spp., this study is therefore focused on the isolation and characterization of bifidobacterial strains from solitary bees, in particular carpenter bee (Xylocopa violacea), builder bee (Osmia cornuta), and red mason bee (Osmia rufa).
30551956	5	56	theme	Osmia	756:760	arg1	rufa					762:765	Osmia rufa	756:765	Osmia rufa	756:765	To date there is sparse information about bifidobacteria in solitary bees such as Xylocopa and Osmia spp., this study is therefore focused on the isolation and characterization of bifidobacterial strains from solitary bees, in particular carpenter bee (Xylocopa violacea), builder bee (Osmia cornuta), and red mason bee (Osmia rufa).
30551956	11	57	theme	reference	1495:1503	arg1	strains					1505:1511	reference strains	1495:1511	reference strains	1495:1511	Phenotypic tests were performed for the proposed type strains, reference strains and their closest neighbor in the phylogenetic tree.
30551956	4	58	theme	solitary	420:427	arg1	bees					429:432	wild solitary bees	415:432	wild solitary bees	415:432	Recently, four novel species have been isolated from guts of different bumblebees, thus allowing to suppose that a core bifidobacterial population may be present in wild solitary bees.
30551956	13	59	theme	type	1661:1664	arg1	strain					1666:1671	strain	1666:1671	strain	1666:1671	nov. whose type strain is XV2 (=DSM 104955T=LMG 30142T), reference strain XV16 and Bifidobacterium aemilianum sp.
30551956	2	60	theme	carpenter	85:93	arg1	tract					129:133	the carpenter bee (Xylocopa violacea) digestive tract	81:133	the carpenter bee (Xylocopa violacea) digestive tract	81:133	nov., from the carpenter bee (Xylocopa violacea) digestive tract.
30551956	7	61	theme	putative	919:926	arg1	species					932:938	putative new species	919:938	putative new species	919:938	no new species have been detected whereas among Xylocopa isolates four strains (XV2, XV4, XV10, XV16) belonging to putative new species were found.
30551956	11	62	theme	type	1481:1484	arg1	strains					1486:1492	the proposed type strains	1468:1492	the proposed type strains	1468:1492	Phenotypic tests were performed for the proposed type strains, reference strains and their closest neighbor in the phylogenetic tree.
30551956	12	63	theme	Bifidobacterium	1620:1634	arg1	sp					1646:1647	two novel species Bifidobacterium xylocopae sp	1602:1647	two novel species Bifidobacterium xylocopae sp	1602:1647	The results support the proposal of two novel species Bifidobacterium xylocopae sp.
30551956	7	64	dep	strains	875:881	arg1	XV4					889:891	XV4	889:891	XV4	889:891	no new species have been detected whereas among Xylocopa isolates four strains (XV2, XV4, XV10, XV16) belonging to putative new species were found.
30551956	7	64	dep	strains	875:881	arg1	XV2					884:886	XV2	884:886	XV2	884:886	no new species have been detected whereas among Xylocopa isolates four strains (XV2, XV4, XV10, XV16) belonging to putative new species were found.
30551956	7	64	dep	strains	875:881	arg1	XV16					900:903	XV16	900:903	XV16	900:903	no new species have been detected whereas among Xylocopa isolates four strains (XV2, XV4, XV10, XV16) belonging to putative new species were found.
30551956	7	64	dep	strains	875:881	arg1	XV10					894:897	XV10	894:897	XV10	894:897	no new species have been detected whereas among Xylocopa isolates four strains (XV2, XV4, XV10, XV16) belonging to putative new species were found.
30551956	5	65	theme	builder	708:714	arg1	bee					716:718	builder bee	708:718	builder bee (Osmia cornuta)	708:734	To date there is sparse information about bifidobacteria in solitary bees such as Xylocopa and Osmia spp., this study is therefore focused on the isolation and characterization of bifidobacterial strains from solitary bees, in particular carpenter bee (Xylocopa violacea), builder bee (Osmia cornuta), and red mason bee (Osmia rufa).
30551956	5	65	theme	builder	708:714	arg1	cornuta					727:733	Osmia cornuta	721:733	Osmia cornuta	721:733	To date there is sparse information about bifidobacteria in solitary bees such as Xylocopa and Osmia spp., this study is therefore focused on the isolation and characterization of bifidobacterial strains from solitary bees, in particular carpenter bee (Xylocopa violacea), builder bee (Osmia cornuta), and red mason bee (Osmia rufa).
30551956	5	66	from	bee	716:718	arg1	bees					653:656	solitary bees	644:656	solitary bees	644:656	To date there is sparse information about bifidobacteria in solitary bees such as Xylocopa and Osmia spp., this study is therefore focused on the isolation and characterization of bifidobacterial strains from solitary bees, in particular carpenter bee (Xylocopa violacea), builder bee (Osmia cornuta), and red mason bee (Osmia rufa).
30551956	5	67	theme	solitary	495:502	arg1	bees					504:507	solitary bees	495:507	solitary bees such as Xylocopa	495:524	To date there is sparse information about bifidobacteria in solitary bees such as Xylocopa and Osmia spp., this study is therefore focused on the isolation and characterization of bifidobacterial strains from solitary bees, in particular carpenter bee (Xylocopa violacea), builder bee (Osmia cornuta), and red mason bee (Osmia rufa).
30551956	5	67	theme	solitary	495:502	arg1	Xylocopa					517:524	Xylocopa	517:524	Xylocopa	517:524	To date there is sparse information about bifidobacteria in solitary bees such as Xylocopa and Osmia spp., this study is therefore focused on the isolation and characterization of bifidobacterial strains from solitary bees, in particular carpenter bee (Xylocopa violacea), builder bee (Osmia cornuta), and red mason bee (Osmia rufa).
30551956	12	68	theme	sp	1646:1647	arg1	proposal					1590:1597	the proposal	1586:1597	the proposal of two novel species Bifidobacterium xylocopae sp	1586:1647	The results support the proposal of two novel species Bifidobacterium xylocopae sp.
30551956	11	69	theme	Phenotypic	1432:1441	arg1	tests					1443:1447	Phenotypic tests	1432:1447	Phenotypic tests	1432:1447	Phenotypic tests were performed for the proposed type strains, reference strains and their closest neighbor in the phylogenetic tree.
30551956	4	70	from	bees	429:432	arg1	present					404:410	present	404:410	present	404:410	Recently, four novel species have been isolated from guts of different bumblebees, thus allowing to suppose that a core bifidobacterial population may be present in wild solitary bees.
30551956	4	70	from	bees	429:432	arg1	population					386:395	a core bifidobacterial population	363:395	a core bifidobacterial population	363:395	Recently, four novel species have been isolated from guts of different bumblebees, thus allowing to suppose that a core bifidobacterial population may be present in wild solitary bees.
30551956	5	71	theme	Xylocopa	688:695	arg1	bee					683:685	particular carpenter bee	662:685	particular carpenter bee (Xylocopa violacea)	662:705	To date there is sparse information about bifidobacteria in solitary bees such as Xylocopa and Osmia spp., this study is therefore focused on the isolation and characterization of bifidobacterial strains from solitary bees, in particular carpenter bee (Xylocopa violacea), builder bee (Osmia cornuta), and red mason bee (Osmia rufa).
30551956	5	71	theme	Xylocopa	688:695	arg1	violacea					697:704	Xylocopa violacea	688:704	Xylocopa violacea	688:704	To date there is sparse information about bifidobacteria in solitary bees such as Xylocopa and Osmia spp., this study is therefore focused on the isolation and characterization of bifidobacterial strains from solitary bees, in particular carpenter bee (Xylocopa violacea), builder bee (Osmia cornuta), and red mason bee (Osmia rufa).
30551956	12	72	theme	novel	1606:1610	arg1	species					1612:1618	two novel species	1602:1618	two novel species Bifidobacterium xylocopae sp	1602:1647	The results support the proposal of two novel species Bifidobacterium xylocopae sp.
30551956	10	73	theme	complete	1219:1226	arg1	sequences					1237:1245	partial and complete 16S rRNA sequences	1207:1245	partial and complete 16S rRNA sequences	1207:1245	Phylogenetic relationships were determined using partial and complete 16S rRNA sequences and hsp60 restriction analysis that confirmed the belonging of the new strains to Bifidobacterium genus and the relatedness of the strains XV2 and XV10 with XV16 and XV4, respectively.
30551956	4	74	theme	novel	265:269	arg1	species					271:277	four novel species	260:277	four novel species	260:277	Recently, four novel species have been isolated from guts of different bumblebees, thus allowing to suppose that a core bifidobacterial population may be present in wild solitary bees.
30551956	3	75	theme	mutualistic	174:184	arg1	bacteria					186:193	gut mutualistic bacteria	170:193	gut mutualistic bacteria	170:193	Social bees harbor a community of gut mutualistic bacteria, among which bifidobacteria occupy an important niche.
30551956	10	76	theme	partial	1207:1213	arg1	sequences					1237:1245	partial and complete 16S rRNA sequences	1207:1245	partial and complete 16S rRNA sequences	1207:1245	Phylogenetic relationships were determined using partial and complete 16S rRNA sequences and hsp60 restriction analysis that confirmed the belonging of the new strains to Bifidobacterium genus and the relatedness of the strains XV2 and XV10 with XV16 and XV4, respectively.
30551956	0	77	theme	xylocopae	16:24	arg1	sp					26:27	Bifidobacterium xylocopae sp	0:27	Bifidobacterium xylocopae sp.	0:28	Bifidobacterium xylocopae sp.
30551956	7	78	theme	Xylocopa	852:859	arg1	isolates					861:868	Xylocopa isolates	852:868	Xylocopa isolates	852:868	no new species have been detected whereas among Xylocopa isolates four strains (XV2, XV4, XV10, XV16) belonging to putative new species were found.
30551956	13	79	theme	Bifidobacterium	1733:1747	arg1	sp					1760:1761	Bifidobacterium aemilianum sp	1733:1761	Bifidobacterium aemilianum sp	1733:1761	nov. whose type strain is XV2 (=DSM 104955T=LMG 30142T), reference strain XV16 and Bifidobacterium aemilianum sp.
30551956	5	80	from	characterization	595:610	arg1	bees					653:656	solitary bees	644:656	solitary bees	644:656	To date there is sparse information about bifidobacteria in solitary bees such as Xylocopa and Osmia spp., this study is therefore focused on the isolation and characterization of bifidobacterial strains from solitary bees, in particular carpenter bee (Xylocopa violacea), builder bee (Osmia cornuta), and red mason bee (Osmia rufa).
30551956	10	81	theme	rRNA	1232:1235	arg1	sequences					1237:1245	partial and complete 16S rRNA sequences	1207:1245	partial and complete 16S rRNA sequences	1207:1245	Phylogenetic relationships were determined using partial and complete 16S rRNA sequences and hsp60 restriction analysis that confirmed the belonging of the new strains to Bifidobacterium genus and the relatedness of the strains XV2 and XV10 with XV16 and XV4, respectively.
30551956	5	82	from	isolation	581:589	arg1	bees					653:656	solitary bees	644:656	solitary bees	644:656	To date there is sparse information about bifidobacteria in solitary bees such as Xylocopa and Osmia spp., this study is therefore focused on the isolation and characterization of bifidobacterial strains from solitary bees, in particular carpenter bee (Xylocopa violacea), builder bee (Osmia cornuta), and red mason bee (Osmia rufa).
30551956	10	83	dep	strains	1378:1384	arg1	strains					1378:1384	the strains XV2 and XV10	1374:1397	the strains XV2 and XV10	1374:1397	Phylogenetic relationships were determined using partial and complete 16S rRNA sequences and hsp60 restriction analysis that confirmed the belonging of the new strains to Bifidobacterium genus and the relatedness of the strains XV2 and XV10 with XV16 and XV4, respectively.
30551956	10	83	dep	strains	1378:1384	arg1	XV10					1394:1397	XV10	1394:1397	XV10	1394:1397	Phylogenetic relationships were determined using partial and complete 16S rRNA sequences and hsp60 restriction analysis that confirmed the belonging of the new strains to Bifidobacterium genus and the relatedness of the strains XV2 and XV10 with XV16 and XV4, respectively.
30551956	10	83	dep	strains	1378:1384	arg1	XV2					1386:1388	XV2	1386:1388	XV2	1386:1388	Phylogenetic relationships were determined using partial and complete 16S rRNA sequences and hsp60 restriction analysis that confirmed the belonging of the new strains to Bifidobacterium genus and the relatedness of the strains XV2 and XV10 with XV16 and XV4, respectively.
30551956	4	84	theme	different	311:319	arg1	bumblebees					321:330	different bumblebees	311:330	different bumblebees	311:330	Recently, four novel species have been isolated from guts of different bumblebees, thus allowing to suppose that a core bifidobacterial population may be present in wild solitary bees.
30551956	10	85	theme	restriction	1257:1267	arg1	analysis					1269:1276	hsp60 restriction analysis	1251:1276	hsp60 restriction analysis	1251:1276	Phylogenetic relationships were determined using partial and complete 16S rRNA sequences and hsp60 restriction analysis that confirmed the belonging of the new strains to Bifidobacterium genus and the relatedness of the strains XV2 and XV10 with XV16 and XV4, respectively.
30551956	7	86	theme	new	807:809	arg1	species					811:817	no new species	804:817	no new species	804:817	no new species have been detected whereas among Xylocopa isolates four strains (XV2, XV4, XV10, XV16) belonging to putative new species were found.
30551956	1	87	theme	Bifidobacterium	39:53	arg1	aemilianum					55:64	Bifidobacterium aemilianum	39:64	Bifidobacterium aemilianum	39:64	nov. and Bifidobacterium aemilianum sp.
30551956	11	88	theme	closest	1523:1529	arg1	neighbor					1531:1538	their closest neighbor	1517:1538	their closest neighbor	1517:1538	Phenotypic tests were performed for the proposed type strains, reference strains and their closest neighbor in the phylogenetic tree.
30551956	5	89	theme	red	741:743	arg1	bee					751:753	red mason bee	741:753	red mason bee (Osmia rufa)	741:766	To date there is sparse information about bifidobacteria in solitary bees such as Xylocopa and Osmia spp., this study is therefore focused on the isolation and characterization of bifidobacterial strains from solitary bees, in particular carpenter bee (Xylocopa violacea), builder bee (Osmia cornuta), and red mason bee (Osmia rufa).
30551956	5	89	theme	red	741:743	arg1	rufa					762:765	Osmia rufa	756:765	Osmia rufa	756:765	To date there is sparse information about bifidobacteria in solitary bees such as Xylocopa and Osmia spp., this study is therefore focused on the isolation and characterization of bifidobacterial strains from solitary bees, in particular carpenter bee (Xylocopa violacea), builder bee (Osmia cornuta), and red mason bee (Osmia rufa).
30551956	5	90	from	bees	653:656	arg1	bee					683:685	particular carpenter bee	662:685	particular carpenter bee (Xylocopa violacea)	662:705	To date there is sparse information about bifidobacteria in solitary bees such as Xylocopa and Osmia spp., this study is therefore focused on the isolation and characterization of bifidobacterial strains from solitary bees, in particular carpenter bee (Xylocopa violacea), builder bee (Osmia cornuta), and red mason bee (Osmia rufa).
30551956	5	90	from	bees	653:656	arg1	isolation					581:589	the isolation	577:589	the isolation	577:589	To date there is sparse information about bifidobacteria in solitary bees such as Xylocopa and Osmia spp., this study is therefore focused on the isolation and characterization of bifidobacterial strains from solitary bees, in particular carpenter bee (Xylocopa violacea), builder bee (Osmia cornuta), and red mason bee (Osmia rufa).
30551956	5	90	from	bees	653:656	arg1	bee					716:718	builder bee	708:718	builder bee (Osmia cornuta)	708:734	To date there is sparse information about bifidobacteria in solitary bees such as Xylocopa and Osmia spp., this study is therefore focused on the isolation and characterization of bifidobacterial strains from solitary bees, in particular carpenter bee (Xylocopa violacea), builder bee (Osmia cornuta), and red mason bee (Osmia rufa).
30551956	5	90	from	bees	653:656	arg1	characterization					595:610	characterization	595:610	characterization	595:610	To date there is sparse information about bifidobacteria in solitary bees such as Xylocopa and Osmia spp., this study is therefore focused on the isolation and characterization of bifidobacterial strains from solitary bees, in particular carpenter bee (Xylocopa violacea), builder bee (Osmia cornuta), and red mason bee (Osmia rufa).
30551956	5	90	from	bees	653:656	arg1	bee					751:753	red mason bee	741:753	red mason bee (Osmia rufa)	741:766	To date there is sparse information about bifidobacteria in solitary bees such as Xylocopa and Osmia spp., this study is therefore focused on the isolation and characterization of bifidobacterial strains from solitary bees, in particular carpenter bee (Xylocopa violacea), builder bee (Osmia cornuta), and red mason bee (Osmia rufa).
30551956	5	90	from	bees	653:656	arg1	violacea					697:704	Xylocopa violacea	688:704	Xylocopa violacea	688:704	To date there is sparse information about bifidobacteria in solitary bees such as Xylocopa and Osmia spp., this study is therefore focused on the isolation and characterization of bifidobacterial strains from solitary bees, in particular carpenter bee (Xylocopa violacea), builder bee (Osmia cornuta), and red mason bee (Osmia rufa).
30551956	5	90	from	bees	653:656	arg1	cornuta					727:733	Osmia cornuta	721:733	Osmia cornuta	721:733	To date there is sparse information about bifidobacteria in solitary bees such as Xylocopa and Osmia spp., this study is therefore focused on the isolation and characterization of bifidobacterial strains from solitary bees, in particular carpenter bee (Xylocopa violacea), builder bee (Osmia cornuta), and red mason bee (Osmia rufa).
30551956	5	90	from	bees	653:656	arg1	strains					631:637	bifidobacterial strains	615:637	bifidobacterial strains from solitary bees, in particular carpenter bee (Xylocopa violacea)	615:705	To date there is sparse information about bifidobacteria in solitary bees such as Xylocopa and Osmia spp., this study is therefore focused on the isolation and characterization of bifidobacterial strains from solitary bees, in particular carpenter bee (Xylocopa violacea), builder bee (Osmia cornuta), and red mason bee (Osmia rufa).
30551956	5	90	from	bees	653:656	arg1	rufa					762:765	Osmia rufa	756:765	Osmia rufa	756:765	To date there is sparse information about bifidobacteria in solitary bees such as Xylocopa and Osmia spp., this study is therefore focused on the isolation and characterization of bifidobacterial strains from solitary bees, in particular carpenter bee (Xylocopa violacea), builder bee (Osmia cornuta), and red mason bee (Osmia rufa).
31896054	5	0	dep	epithelia	1093:1101	arg1	damages					1103:1109	damages	1103:1109	damages	1103:1109	RESULTS It was observed that FOSs alleviated depression-like behaviors and repaired intestinal epithelia damages.
31896054	2	1	theme	microbiota-gut-brain	373:392	arg1	axis					394:397	the microbiota-gut-brain axis	369:397	the microbiota-gut-brain axis	369:397	PURPOSE Therefore, the microbiota-gut-brain axis was used to investigate the antidepressive properties of FOSs at the interface of the gut microbiota (GM).
31896054	7	2	theme	depression-associated	1459:1479	arg1	bacteria					1481:1488	depression-associated bacteria	1459:1488	depression-associated bacteria (e.g., Anaerostipes, Oscillibacter, Proteobacteria, and Streptococcus)	1459:1559	Moreover, the GM compositions of normal and model rats were distantly clustered and were mainly related to the disappearance of beneficial bacteria (e.g., Acinetobacter, Barnesiella, Coprococcus, Dialister, Lactobacillus, and Paenibacillus) and appearance of depression-associated bacteria (e.g., Anaerostipes, Oscillibacter, Proteobacteria, and Streptococcus) in depressive rats.
31896054	10	3	theme	FOSs	1986:1989	arg1	component					1973:1981	a component	1971:1981	a component of FOSs, degrees of polymerization (DP) 5, and fluoxetine, the standard antidepressant drug	1971:2073	Apparently, FOSs-induced modulation of GM was more antidepressive compared to a component of FOSs, degrees of polymerization (DP) 5, and fluoxetine, the standard antidepressant drug.
31896054	7	4	from	rats	1575:1578	arg1	disappearance					1311:1323	the disappearance	1307:1323	the disappearance of beneficial bacteria (e.g., Acinetobacter, Barnesiella, Coprococcus, Dialister, Lactobacillus, and Paenibacillus) and appearance of depression-associated bacteria (e.g., Anaerostipes, Oscillibacter, Proteobacteria, and Streptococcus) in depressive rats	1307:1578	Moreover, the GM compositions of normal and model rats were distantly clustered and were mainly related to the disappearance of beneficial bacteria (e.g., Acinetobacter, Barnesiella, Coprococcus, Dialister, Lactobacillus, and Paenibacillus) and appearance of depression-associated bacteria (e.g., Anaerostipes, Oscillibacter, Proteobacteria, and Streptococcus) in depressive rats.
31896054	4	5	theme	intergenic	869:878	arg1	-PCR					896:899	enterobacterial repetitive intergenic consensus (ERIC)-PCR	842:899	enterobacterial repetitive intergenic consensus (ERIC)-PCR analysis	842:908	Bacterial genomic DNA was extracted from feces, and the GM was profiled for using enterobacterial repetitive intergenic consensus (ERIC)-PCR analysis, partial least squares-discriminant analysis (PLS-DA) and 16S rRNA gene pyrosequencing.
31896054	7	6	theme	appearance	1445:1454	arg1	disappearance					1311:1323	the disappearance	1307:1323	the disappearance of beneficial bacteria (e.g., Acinetobacter, Barnesiella, Coprococcus, Dialister, Lactobacillus, and Paenibacillus) and appearance of depression-associated bacteria (e.g., Anaerostipes, Oscillibacter, Proteobacteria, and Streptococcus) in depressive rats	1307:1578	Moreover, the GM compositions of normal and model rats were distantly clustered and were mainly related to the disappearance of beneficial bacteria (e.g., Acinetobacter, Barnesiella, Coprococcus, Dialister, Lactobacillus, and Paenibacillus) and appearance of depression-associated bacteria (e.g., Anaerostipes, Oscillibacter, Proteobacteria, and Streptococcus) in depressive rats.
31896054	11	7	theme	FOSs	2153:2156	arg1	efficacy					2141:2148	antidepressant efficacy	2126:2148	antidepressant efficacy of FOSs	2126:2156	CONCLUSION In conclusion, this study implied that antidepressant efficacy of FOSs was inseparable from and strongly associated with the modulation of the host' s GM.
31896054	10	8	theme	polymerization	2003:2016	arg1	fluoxetine					2030:2039	fluoxetine	2030:2039	fluoxetine	2030:2039	Apparently, FOSs-induced modulation of GM was more antidepressive compared to a component of FOSs, degrees of polymerization (DP) 5, and fluoxetine, the standard antidepressant drug.
31896054	10	8	theme	polymerization	2003:2016	arg1	drug					2070:2073	the standard antidepressant drug	2042:2073	the standard antidepressant drug	2042:2073	Apparently, FOSs-induced modulation of GM was more antidepressive compared to a component of FOSs, degrees of polymerization (DP) 5, and fluoxetine, the standard antidepressant drug.
31896054	10	8	theme	polymerization	2003:2016	arg1	degrees					1992:1998	degrees	1992:1998	degrees of polymerization (DP) 5	1992:2023	Apparently, FOSs-induced modulation of GM was more antidepressive compared to a component of FOSs, degrees of polymerization (DP) 5, and fluoxetine, the standard antidepressant drug.
31896054	10	8	theme	polymerization	2003:2016	arg1	FOSs					1986:1989	FOSs	1986:1989	FOSs	1986:1989	Apparently, FOSs-induced modulation of GM was more antidepressive compared to a component of FOSs, degrees of polymerization (DP) 5, and fluoxetine, the standard antidepressant drug.
31896054	9	9	theme	phylum	1725:1730	arg1	bacteria					1758:1765	bacteria	1758:1765	bacteria known for the secretion of pharmacologically important metabolites, such as H2S, that exhibit antidepressant-like properties	1758:1890	Notably, FOSs promoted the abundance of the bacterial phylum Cyanobacteria, a group of bacteria known for the secretion of pharmacologically important metabolites, such as H2S, that exhibit antidepressant-like properties.
31896054	9	9	theme	phylum	1725:1730	arg1	Cyanobacteria					1732:1744	the bacterial phylum Cyanobacteria	1711:1744	the bacterial phylum Cyanobacteria	1711:1744	Notably, FOSs promoted the abundance of the bacterial phylum Cyanobacteria, a group of bacteria known for the secretion of pharmacologically important metabolites, such as H2S, that exhibit antidepressant-like properties.
31896054	9	9	theme	phylum	1725:1730	arg1	group					1749:1753	a group	1747:1753	a group of bacteria known for the secretion of pharmacologically important metabolites, such as H2S, that exhibit antidepressant-like properties	1747:1890	Notably, FOSs promoted the abundance of the bacterial phylum Cyanobacteria, a group of bacteria known for the secretion of pharmacologically important metabolites, such as H2S, that exhibit antidepressant-like properties.
31896054	7	10	from	disappearance	1311:1323	arg1	rats					1575:1578	depressive rats	1564:1578	depressive rats	1564:1578	Moreover, the GM compositions of normal and model rats were distantly clustered and were mainly related to the disappearance of beneficial bacteria (e.g., Acinetobacter, Barnesiella, Coprococcus, Dialister, Lactobacillus, and Paenibacillus) and appearance of depression-associated bacteria (e.g., Anaerostipes, Oscillibacter, Proteobacteria, and Streptococcus) in depressive rats.
31896054	6	11	theme	model	1188:1192	arg1	rats					1194:1197	the model rats	1184:1197	the model rats	1184:1197	FOSs treatment lowered corticosterone levels in the plasma and urine of the model rats.
31896054	4	12	theme	Bacterial	760:768	arg1	DNA					778:780	Bacterial genomic DNA	760:780	Bacterial genomic DNA	760:780	Bacterial genomic DNA was extracted from feces, and the GM was profiled for using enterobacterial repetitive intergenic consensus (ERIC)-PCR analysis, partial least squares-discriminant analysis (PLS-DA) and 16S rRNA gene pyrosequencing.
31896054	10	13	theme	degrees	1992:1998	arg1	component					1973:1981	a component	1971:1981	a component of FOSs, degrees of polymerization (DP) 5, and fluoxetine, the standard antidepressant drug	1971:2073	Apparently, FOSs-induced modulation of GM was more antidepressive compared to a component of FOSs, degrees of polymerization (DP) 5, and fluoxetine, the standard antidepressant drug.
31896054	4	14	theme	partial	911:917	arg1	PLS-DA					956:961	PLS-DA	956:961	PLS-DA	956:961	Bacterial genomic DNA was extracted from feces, and the GM was profiled for using enterobacterial repetitive intergenic consensus (ERIC)-PCR analysis, partial least squares-discriminant analysis (PLS-DA) and 16S rRNA gene pyrosequencing.
31896054	4	14	theme	partial	911:917	arg1	analysis					946:953	partial least squares-discriminant analysis	911:953	partial least squares-discriminant analysis (PLS-DA)	911:962	Bacterial genomic DNA was extracted from feces, and the GM was profiled for using enterobacterial repetitive intergenic consensus (ERIC)-PCR analysis, partial least squares-discriminant analysis (PLS-DA) and 16S rRNA gene pyrosequencing.
31896054	4	15	theme	squares-discriminant	925:944	arg1	PLS-DA					956:961	PLS-DA	956:961	PLS-DA	956:961	Bacterial genomic DNA was extracted from feces, and the GM was profiled for using enterobacterial repetitive intergenic consensus (ERIC)-PCR analysis, partial least squares-discriminant analysis (PLS-DA) and 16S rRNA gene pyrosequencing.
31896054	4	15	theme	squares-discriminant	925:944	arg1	analysis					946:953	partial least squares-discriminant analysis	911:953	partial least squares-discriminant analysis (PLS-DA)	911:962	Bacterial genomic DNA was extracted from feces, and the GM was profiled for using enterobacterial repetitive intergenic consensus (ERIC)-PCR analysis, partial least squares-discriminant analysis (PLS-DA) and 16S rRNA gene pyrosequencing.
31896054	5	16	dep	RESULTS	998:1004	arg1	observed					1013:1020	observed	1013:1020	was observed that FOSs alleviated depression-like behaviors and repaired intestinal epithelia damages	1009:1109	RESULTS It was observed that FOSs alleviated depression-like behaviors and repaired intestinal epithelia damages.
31896054	5	17	theme	repaired	1073:1080	arg1	epithelia					1093:1101	repaired intestinal epithelia damages	1073:1109	repaired intestinal epithelia damages	1073:1109	RESULTS It was observed that FOSs alleviated depression-like behaviors and repaired intestinal epithelia damages.
31896054	3	18	theme	unpredictable	602:614	arg1	CUMS					629:632	CUMS	629:632	CUMS	629:632	STUDY DESIGN AND METHODS FOSs was introduced via intragastric gavage to rats exposed to chronic unpredictable mild stress (CUMS), and the antidepressive effects were investigated through behavioral tests, intestinal morphology and corticosterone levels.
31896054	3	18	theme	unpredictable	602:614	arg1	stress					621:626	chronic unpredictable mild stress	594:626	chronic unpredictable mild stress (CUMS)	594:633	STUDY DESIGN AND METHODS FOSs was introduced via intragastric gavage to rats exposed to chronic unpredictable mild stress (CUMS), and the antidepressive effects were investigated through behavioral tests, intestinal morphology and corticosterone levels.
31896054	1	19	theme	BACKGROUND	132:141	arg1	FOSs					180:183	FOSs	180:183	FOSs	180:183	BACKGROUND Inulin-type fructo-oligosaccharides (FOSs) purified from Morinda officinalis How., an effective oral antidepressant for mild to moderate depression, have a largely unknown efficacy and poor bioavailability.
31896054	1	19	theme	BACKGROUND	132:141	arg1	antidepressant					244:257	antidepressant	244:257	antidepressant	244:257	BACKGROUND Inulin-type fructo-oligosaccharides (FOSs) purified from Morinda officinalis How., an effective oral antidepressant for mild to moderate depression, have a largely unknown efficacy and poor bioavailability.
31896054	1	19	theme	BACKGROUND	132:141	arg1	fructo-oligosaccharides					155:177	BACKGROUND Inulin-type fructo-oligosaccharides	132:177	BACKGROUND Inulin-type fructo-oligosaccharides (FOSs) purified from Morinda officinalis How.	132:223	BACKGROUND Inulin-type fructo-oligosaccharides (FOSs) purified from Morinda officinalis How., an effective oral antidepressant for mild to moderate depression, have a largely unknown efficacy and poor bioavailability.
31896054	9	20	theme	bacteria	1758:1765	arg1	bacteria					1758:1765	bacteria	1758:1765	bacteria known for the secretion of pharmacologically important metabolites, such as H2S, that exhibit antidepressant-like properties	1758:1890	Notably, FOSs promoted the abundance of the bacterial phylum Cyanobacteria, a group of bacteria known for the secretion of pharmacologically important metabolites, such as H2S, that exhibit antidepressant-like properties.
31896054	9	20	theme	bacteria	1758:1765	arg1	Cyanobacteria					1732:1744	the bacterial phylum Cyanobacteria	1711:1744	the bacterial phylum Cyanobacteria	1711:1744	Notably, FOSs promoted the abundance of the bacterial phylum Cyanobacteria, a group of bacteria known for the secretion of pharmacologically important metabolites, such as H2S, that exhibit antidepressant-like properties.
31896054	9	20	theme	bacteria	1758:1765	arg1	group					1749:1753	a group	1747:1753	a group of bacteria known for the secretion of pharmacologically important metabolites, such as H2S, that exhibit antidepressant-like properties	1747:1890	Notably, FOSs promoted the abundance of the bacterial phylum Cyanobacteria, a group of bacteria known for the secretion of pharmacologically important metabolites, such as H2S, that exhibit antidepressant-like properties.
31896054	11	21	theme	s	2236:2236	arg1	GM					2238:2239	the host' s GM	2226:2239	the host' s GM	2226:2239	CONCLUSION In conclusion, this study implied that antidepressant efficacy of FOSs was inseparable from and strongly associated with the modulation of the host' s GM.
31896054	7	22	from	appearance	1445:1454	arg1	rats					1575:1578	depressive rats	1564:1578	depressive rats	1564:1578	Moreover, the GM compositions of normal and model rats were distantly clustered and were mainly related to the disappearance of beneficial bacteria (e.g., Acinetobacter, Barnesiella, Coprococcus, Dialister, Lactobacillus, and Paenibacillus) and appearance of depression-associated bacteria (e.g., Anaerostipes, Oscillibacter, Proteobacteria, and Streptococcus) in depressive rats.
31896054	11	23	theme	host	2230:2233	arg1	GM					2238:2239	the host' s GM	2226:2239	the host' s GM	2226:2239	CONCLUSION In conclusion, this study implied that antidepressant efficacy of FOSs was inseparable from and strongly associated with the modulation of the host' s GM.
31896054	7	24	theme	rats	1250:1253	arg1	compositions					1217:1228	the GM compositions	1210:1228	the GM compositions of normal and model rats	1210:1253	Moreover, the GM compositions of normal and model rats were distantly clustered and were mainly related to the disappearance of beneficial bacteria (e.g., Acinetobacter, Barnesiella, Coprococcus, Dialister, Lactobacillus, and Paenibacillus) and appearance of depression-associated bacteria (e.g., Anaerostipes, Oscillibacter, Proteobacteria, and Streptococcus) in depressive rats.
31896054	7	24	theme	rats	1250:1253	arg1	related					1296:1302	related	1296:1302	related	1296:1302	Moreover, the GM compositions of normal and model rats were distantly clustered and were mainly related to the disappearance of beneficial bacteria (e.g., Acinetobacter, Barnesiella, Coprococcus, Dialister, Lactobacillus, and Paenibacillus) and appearance of depression-associated bacteria (e.g., Anaerostipes, Oscillibacter, Proteobacteria, and Streptococcus) in depressive rats.
31896054	11	25	theme	antidepressant	2126:2139	arg1	efficacy					2141:2148	antidepressant efficacy	2126:2148	antidepressant efficacy of FOSs	2126:2156	CONCLUSION In conclusion, this study implied that antidepressant efficacy of FOSs was inseparable from and strongly associated with the modulation of the host' s GM.
31896054	10	26	theme	FOSs-induced	1905:1916	arg1	modulation					1918:1927	FOSs-induced modulation	1905:1927	FOSs-induced modulation of GM	1905:1933	Apparently, FOSs-induced modulation of GM was more antidepressive compared to a component of FOSs, degrees of polymerization (DP) 5, and fluoxetine, the standard antidepressant drug.
31896054	4	27	theme	16S	968:970	arg1	rRNA					972:975	16S rRNA	968:975	16S rRNA gene pyrosequencing	968:995	Bacterial genomic DNA was extracted from feces, and the GM was profiled for using enterobacterial repetitive intergenic consensus (ERIC)-PCR analysis, partial least squares-discriminant analysis (PLS-DA) and 16S rRNA gene pyrosequencing.
31896054	10	28	theme	standard	2046:2053	arg1	fluoxetine					2030:2039	fluoxetine	2030:2039	fluoxetine	2030:2039	Apparently, FOSs-induced modulation of GM was more antidepressive compared to a component of FOSs, degrees of polymerization (DP) 5, and fluoxetine, the standard antidepressant drug.
31896054	10	28	theme	standard	2046:2053	arg1	drug					2070:2073	the standard antidepressant drug	2042:2073	the standard antidepressant drug	2042:2073	Apparently, FOSs-induced modulation of GM was more antidepressive compared to a component of FOSs, degrees of polymerization (DP) 5, and fluoxetine, the standard antidepressant drug.
31896054	4	29	theme	gene	977:980	arg1	pyrosequencing					982:995	16S rRNA gene pyrosequencing	968:995	16S rRNA gene pyrosequencing	968:995	Bacterial genomic DNA was extracted from feces, and the GM was profiled for using enterobacterial repetitive intergenic consensus (ERIC)-PCR analysis, partial least squares-discriminant analysis (PLS-DA) and 16S rRNA gene pyrosequencing.
31896054	2	30	used	used	403:406	arg2	axis					394:397	the microbiota-gut-brain axis	369:397	the microbiota-gut-brain axis	369:397	PURPOSE Therefore, the microbiota-gut-brain axis was used to investigate the antidepressive properties of FOSs at the interface of the gut microbiota (GM).
31896054	2	31	theme	antidepressive	427:440	arg1	properties					442:451	the antidepressive properties	423:451	the antidepressive properties of FOSs	423:459	PURPOSE Therefore, the microbiota-gut-brain axis was used to investigate the antidepressive properties of FOSs at the interface of the gut microbiota (GM).
31896054	0	32	theme	rat	121:123	arg1	model					125:129	a stress rat model	112:129	a stress rat model	112:129	Fructo-oligosaccharides from Morinda officinalis remodeled gut microbiota and alleviated depression features in a stress rat model.
31896054	3	33	dep	DESIGN	512:517	arg1	FOSs					531:534	FOSs	531:534	FOSs	531:534	STUDY DESIGN AND METHODS FOSs was introduced via intragastric gavage to rats exposed to chronic unpredictable mild stress (CUMS), and the antidepressive effects were investigated through behavioral tests, intestinal morphology and corticosterone levels.
31896054	4	34	theme	genomic	770:776	arg1	DNA					778:780	Bacterial genomic DNA	760:780	Bacterial genomic DNA	760:780	Bacterial genomic DNA was extracted from feces, and the GM was profiled for using enterobacterial repetitive intergenic consensus (ERIC)-PCR analysis, partial least squares-discriminant analysis (PLS-DA) and 16S rRNA gene pyrosequencing.
31896054	7	35	theme	GM	1214:1215	arg1	compositions					1217:1228	the GM compositions	1210:1228	the GM compositions of normal and model rats	1210:1253	Moreover, the GM compositions of normal and model rats were distantly clustered and were mainly related to the disappearance of beneficial bacteria (e.g., Acinetobacter, Barnesiella, Coprococcus, Dialister, Lactobacillus, and Paenibacillus) and appearance of depression-associated bacteria (e.g., Anaerostipes, Oscillibacter, Proteobacteria, and Streptococcus) in depressive rats.
31896054	7	35	theme	GM	1214:1215	arg1	related					1296:1302	related	1296:1302	related	1296:1302	Moreover, the GM compositions of normal and model rats were distantly clustered and were mainly related to the disappearance of beneficial bacteria (e.g., Acinetobacter, Barnesiella, Coprococcus, Dialister, Lactobacillus, and Paenibacillus) and appearance of depression-associated bacteria (e.g., Anaerostipes, Oscillibacter, Proteobacteria, and Streptococcus) in depressive rats.
31896054	0	36	theme	Morinda	29:35	arg1	officinalis					37:47	Morinda officinalis	29:47	Morinda officinalis	29:47	Fructo-oligosaccharides from Morinda officinalis remodeled gut microbiota and alleviated depression features in a stress rat model.
31896054	1	37	theme	unknown	307:313	arg1	efficacy					315:322	a largely unknown efficacy	297:322	a largely unknown efficacy	297:322	BACKGROUND Inulin-type fructo-oligosaccharides (FOSs) purified from Morinda officinalis How., an effective oral antidepressant for mild to moderate depression, have a largely unknown efficacy and poor bioavailability.
31896054	5	38	theme	depression-like	1043:1057	arg1	behaviors					1059:1067	depression-like behaviors	1043:1067	depression-like behaviors	1043:1067	RESULTS It was observed that FOSs alleviated depression-like behaviors and repaired intestinal epithelia damages.
31896054	7	39	theme	beneficial	1328:1337	arg1	bacteria					1339:1346	beneficial bacteria	1328:1346	beneficial bacteria (e.g., Acinetobacter, Barnesiella, Coprococcus, Dialister, Lactobacillus, and Paenibacillus)	1328:1439	Moreover, the GM compositions of normal and model rats were distantly clustered and were mainly related to the disappearance of beneficial bacteria (e.g., Acinetobacter, Barnesiella, Coprococcus, Dialister, Lactobacillus, and Paenibacillus) and appearance of depression-associated bacteria (e.g., Anaerostipes, Oscillibacter, Proteobacteria, and Streptococcus) in depressive rats.
31896054	7	39	theme	beneficial	1328:1337	arg1	Barnesiella					1370:1380	Barnesiella	1370:1380	Barnesiella	1370:1380	Moreover, the GM compositions of normal and model rats were distantly clustered and were mainly related to the disappearance of beneficial bacteria (e.g., Acinetobacter, Barnesiella, Coprococcus, Dialister, Lactobacillus, and Paenibacillus) and appearance of depression-associated bacteria (e.g., Anaerostipes, Oscillibacter, Proteobacteria, and Streptococcus) in depressive rats.
31896054	7	39	theme	beneficial	1328:1337	arg1	Lactobacillus					1407:1419	Lactobacillus	1407:1419	Lactobacillus	1407:1419	Moreover, the GM compositions of normal and model rats were distantly clustered and were mainly related to the disappearance of beneficial bacteria (e.g., Acinetobacter, Barnesiella, Coprococcus, Dialister, Lactobacillus, and Paenibacillus) and appearance of depression-associated bacteria (e.g., Anaerostipes, Oscillibacter, Proteobacteria, and Streptococcus) in depressive rats.
31896054	7	39	theme	beneficial	1328:1337	arg1	Dialister					1396:1404	Dialister	1396:1404	Dialister	1396:1404	Moreover, the GM compositions of normal and model rats were distantly clustered and were mainly related to the disappearance of beneficial bacteria (e.g., Acinetobacter, Barnesiella, Coprococcus, Dialister, Lactobacillus, and Paenibacillus) and appearance of depression-associated bacteria (e.g., Anaerostipes, Oscillibacter, Proteobacteria, and Streptococcus) in depressive rats.
31896054	2	40	theme	microbiota	489:498	arg1	interface					468:476	the interface	464:476	the interface of the gut microbiota (GM)	464:503	PURPOSE Therefore, the microbiota-gut-brain axis was used to investigate the antidepressive properties of FOSs at the interface of the gut microbiota (GM).
31896054	4	41	theme	enterobacterial	842:856	arg1	-PCR					896:899	enterobacterial repetitive intergenic consensus (ERIC)-PCR	842:899	enterobacterial repetitive intergenic consensus (ERIC)-PCR analysis	842:908	Bacterial genomic DNA was extracted from feces, and the GM was profiled for using enterobacterial repetitive intergenic consensus (ERIC)-PCR analysis, partial least squares-discriminant analysis (PLS-DA) and 16S rRNA gene pyrosequencing.
31896054	3	42	theme	STUDY	506:510	arg1	DESIGN					512:517	STUDY DESIGN	506:517	STUDY DESIGN	506:517	STUDY DESIGN AND METHODS FOSs was introduced via intragastric gavage to rats exposed to chronic unpredictable mild stress (CUMS), and the antidepressive effects were investigated through behavioral tests, intestinal morphology and corticosterone levels.
31896054	7	43	from	bacteria	1339:1346	arg1	rats					1575:1578	depressive rats	1564:1578	depressive rats	1564:1578	Moreover, the GM compositions of normal and model rats were distantly clustered and were mainly related to the disappearance of beneficial bacteria (e.g., Acinetobacter, Barnesiella, Coprococcus, Dialister, Lactobacillus, and Paenibacillus) and appearance of depression-associated bacteria (e.g., Anaerostipes, Oscillibacter, Proteobacteria, and Streptococcus) in depressive rats.
31896054	10	44	theme	GM	1932:1933	arg1	modulation					1918:1927	FOSs-induced modulation	1905:1927	FOSs-induced modulation of GM	1905:1933	Apparently, FOSs-induced modulation of GM was more antidepressive compared to a component of FOSs, degrees of polymerization (DP) 5, and fluoxetine, the standard antidepressant drug.
31896054	9	45	theme	antidepressant-like	1861:1879	arg1	properties					1881:1890	antidepressant-like properties	1861:1890	antidepressant-like properties	1861:1890	Notably, FOSs promoted the abundance of the bacterial phylum Cyanobacteria, a group of bacteria known for the secretion of pharmacologically important metabolites, such as H2S, that exhibit antidepressant-like properties.
31896054	8	46	from	dysbiosis	1600:1608	arg1	gut					1630:1632	depressive rats' gut	1613:1632	depressive rats' gut	1613:1632	Interestingly, the dysbiosis in depressive rats' gut was reinstated with FOSs treatments.
31896054	1	47	theme	moderate	271:278	arg1	depression					280:289	mild to moderate depression	263:289	mild to moderate depression	263:289	BACKGROUND Inulin-type fructo-oligosaccharides (FOSs) purified from Morinda officinalis How., an effective oral antidepressant for mild to moderate depression, have a largely unknown efficacy and poor bioavailability.
31896054	4	48	theme	ERIC	891:894	arg1	-PCR					896:899	enterobacterial repetitive intergenic consensus (ERIC)-PCR	842:899	enterobacterial repetitive intergenic consensus (ERIC)-PCR analysis	842:908	Bacterial genomic DNA was extracted from feces, and the GM was profiled for using enterobacterial repetitive intergenic consensus (ERIC)-PCR analysis, partial least squares-discriminant analysis (PLS-DA) and 16S rRNA gene pyrosequencing.
31896054	6	49	theme	corticosterone	1135:1148	arg1	levels					1150:1155	corticosterone levels	1135:1155	corticosterone levels	1135:1155	FOSs treatment lowered corticosterone levels in the plasma and urine of the model rats.
31896054	8	50	theme	FOSs	1654:1657	arg1	treatments					1659:1668	FOSs treatments	1654:1668	FOSs treatments	1654:1668	Interestingly, the dysbiosis in depressive rats' gut was reinstated with FOSs treatments.
31896054	3	51	theme	intragastric	555:566	arg1	gavage					568:573	intragastric gavage	555:573	intragastric gavage to rats exposed to chronic unpredictable mild stress (CUMS)	555:633	STUDY DESIGN AND METHODS FOSs was introduced via intragastric gavage to rats exposed to chronic unpredictable mild stress (CUMS), and the antidepressive effects were investigated through behavioral tests, intestinal morphology and corticosterone levels.
31896054	4	52	theme	-PCR	896:899	arg1	analysis					901:908	enterobacterial repetitive intergenic consensus (ERIC)-PCR analysis	842:908	enterobacterial repetitive intergenic consensus (ERIC)-PCR analysis	842:908	Bacterial genomic DNA was extracted from feces, and the GM was profiled for using enterobacterial repetitive intergenic consensus (ERIC)-PCR analysis, partial least squares-discriminant analysis (PLS-DA) and 16S rRNA gene pyrosequencing.
31896054	7	53	theme	bacteria	1481:1488	arg1	bacteria					1339:1346	beneficial bacteria	1328:1346	beneficial bacteria (e.g., Acinetobacter, Barnesiella, Coprococcus, Dialister, Lactobacillus, and Paenibacillus)	1328:1439	Moreover, the GM compositions of normal and model rats were distantly clustered and were mainly related to the disappearance of beneficial bacteria (e.g., Acinetobacter, Barnesiella, Coprococcus, Dialister, Lactobacillus, and Paenibacillus) and appearance of depression-associated bacteria (e.g., Anaerostipes, Oscillibacter, Proteobacteria, and Streptococcus) in depressive rats.
31896054	7	53	theme	bacteria	1481:1488	arg1	Barnesiella					1370:1380	Barnesiella	1370:1380	Barnesiella	1370:1380	Moreover, the GM compositions of normal and model rats were distantly clustered and were mainly related to the disappearance of beneficial bacteria (e.g., Acinetobacter, Barnesiella, Coprococcus, Dialister, Lactobacillus, and Paenibacillus) and appearance of depression-associated bacteria (e.g., Anaerostipes, Oscillibacter, Proteobacteria, and Streptococcus) in depressive rats.
31896054	7	53	theme	bacteria	1481:1488	arg1	Lactobacillus					1407:1419	Lactobacillus	1407:1419	Lactobacillus	1407:1419	Moreover, the GM compositions of normal and model rats were distantly clustered and were mainly related to the disappearance of beneficial bacteria (e.g., Acinetobacter, Barnesiella, Coprococcus, Dialister, Lactobacillus, and Paenibacillus) and appearance of depression-associated bacteria (e.g., Anaerostipes, Oscillibacter, Proteobacteria, and Streptococcus) in depressive rats.
31896054	7	53	theme	bacteria	1481:1488	arg1	Dialister					1396:1404	Dialister	1396:1404	Dialister	1396:1404	Moreover, the GM compositions of normal and model rats were distantly clustered and were mainly related to the disappearance of beneficial bacteria (e.g., Acinetobacter, Barnesiella, Coprococcus, Dialister, Lactobacillus, and Paenibacillus) and appearance of depression-associated bacteria (e.g., Anaerostipes, Oscillibacter, Proteobacteria, and Streptococcus) in depressive rats.
31896054	7	53	theme	bacteria	1481:1488	arg1	appearance					1445:1454	appearance	1445:1454	appearance of depression-associated bacteria (e.g., Anaerostipes, Oscillibacter, Proteobacteria, and Streptococcus) in depressive rats	1445:1578	Moreover, the GM compositions of normal and model rats were distantly clustered and were mainly related to the disappearance of beneficial bacteria (e.g., Acinetobacter, Barnesiella, Coprococcus, Dialister, Lactobacillus, and Paenibacillus) and appearance of depression-associated bacteria (e.g., Anaerostipes, Oscillibacter, Proteobacteria, and Streptococcus) in depressive rats.
31896054	4	54	theme	repetitive	858:867	arg1	-PCR					896:899	enterobacterial repetitive intergenic consensus (ERIC)-PCR	842:899	enterobacterial repetitive intergenic consensus (ERIC)-PCR analysis	842:908	Bacterial genomic DNA was extracted from feces, and the GM was profiled for using enterobacterial repetitive intergenic consensus (ERIC)-PCR analysis, partial least squares-discriminant analysis (PLS-DA) and 16S rRNA gene pyrosequencing.
31896054	3	55	theme	corticosterone	737:750	arg1	levels					752:757	corticosterone levels	737:757	corticosterone levels	737:757	STUDY DESIGN AND METHODS FOSs was introduced via intragastric gavage to rats exposed to chronic unpredictable mild stress (CUMS), and the antidepressive effects were investigated through behavioral tests, intestinal morphology and corticosterone levels.
31896054	4	56	theme	consensus	880:888	arg1	-PCR					896:899	enterobacterial repetitive intergenic consensus (ERIC)-PCR	842:899	enterobacterial repetitive intergenic consensus (ERIC)-PCR analysis	842:908	Bacterial genomic DNA was extracted from feces, and the GM was profiled for using enterobacterial repetitive intergenic consensus (ERIC)-PCR analysis, partial least squares-discriminant analysis (PLS-DA) and 16S rRNA gene pyrosequencing.
31896054	6	57	theme	rats	1194:1197	arg1	urine					1175:1179	urine	1175:1179	urine	1175:1179	FOSs treatment lowered corticosterone levels in the plasma and urine of the model rats.
31896054	6	57	theme	rats	1194:1197	arg1	plasma					1164:1169	plasma	1164:1169	plasma	1164:1169	FOSs treatment lowered corticosterone levels in the plasma and urine of the model rats.
31896054	7	58	dep	Barnesiella	1370:1380	arg1	e.g.					1349:1352	e.g.	1349:1352	e.g.	1349:1352	Moreover, the GM compositions of normal and model rats were distantly clustered and were mainly related to the disappearance of beneficial bacteria (e.g., Acinetobacter, Barnesiella, Coprococcus, Dialister, Lactobacillus, and Paenibacillus) and appearance of depression-associated bacteria (e.g., Anaerostipes, Oscillibacter, Proteobacteria, and Streptococcus) in depressive rats.
31896054	9	59	theme	Cyanobacteria	1732:1744	arg1	abundance					1698:1706	the abundance	1694:1706	the abundance of the bacterial phylum Cyanobacteria, a group of bacteria known for the secretion of pharmacologically important metabolites, such as H2S, that exhibit antidepressant-like properties	1694:1890	Notably, FOSs promoted the abundance of the bacterial phylum Cyanobacteria, a group of bacteria known for the secretion of pharmacologically important metabolites, such as H2S, that exhibit antidepressant-like properties.
31896054	9	60	theme	bacterial	1715:1723	arg1	bacteria					1758:1765	bacteria	1758:1765	bacteria known for the secretion of pharmacologically important metabolites, such as H2S, that exhibit antidepressant-like properties	1758:1890	Notably, FOSs promoted the abundance of the bacterial phylum Cyanobacteria, a group of bacteria known for the secretion of pharmacologically important metabolites, such as H2S, that exhibit antidepressant-like properties.
31896054	9	60	theme	bacterial	1715:1723	arg1	Cyanobacteria					1732:1744	the bacterial phylum Cyanobacteria	1711:1744	the bacterial phylum Cyanobacteria	1711:1744	Notably, FOSs promoted the abundance of the bacterial phylum Cyanobacteria, a group of bacteria known for the secretion of pharmacologically important metabolites, such as H2S, that exhibit antidepressant-like properties.
31896054	9	60	theme	bacterial	1715:1723	arg1	group					1749:1753	a group	1747:1753	a group of bacteria known for the secretion of pharmacologically important metabolites, such as H2S, that exhibit antidepressant-like properties	1747:1890	Notably, FOSs promoted the abundance of the bacterial phylum Cyanobacteria, a group of bacteria known for the secretion of pharmacologically important metabolites, such as H2S, that exhibit antidepressant-like properties.
31896054	3	61	theme	mild	616:619	arg1	CUMS					629:632	CUMS	629:632	CUMS	629:632	STUDY DESIGN AND METHODS FOSs was introduced via intragastric gavage to rats exposed to chronic unpredictable mild stress (CUMS), and the antidepressive effects were investigated through behavioral tests, intestinal morphology and corticosterone levels.
31896054	3	61	theme	mild	616:619	arg1	stress					621:626	chronic unpredictable mild stress	594:626	chronic unpredictable mild stress (CUMS)	594:633	STUDY DESIGN AND METHODS FOSs was introduced via intragastric gavage to rats exposed to chronic unpredictable mild stress (CUMS), and the antidepressive effects were investigated through behavioral tests, intestinal morphology and corticosterone levels.
31896054	7	62	theme	normal	1233:1238	arg1	rats					1250:1253	normal and model rats	1233:1253	normal and model rats	1233:1253	Moreover, the GM compositions of normal and model rats were distantly clustered and were mainly related to the disappearance of beneficial bacteria (e.g., Acinetobacter, Barnesiella, Coprococcus, Dialister, Lactobacillus, and Paenibacillus) and appearance of depression-associated bacteria (e.g., Anaerostipes, Oscillibacter, Proteobacteria, and Streptococcus) in depressive rats.
31896054	3	63	theme	chronic	594:600	arg1	CUMS					629:632	CUMS	629:632	CUMS	629:632	STUDY DESIGN AND METHODS FOSs was introduced via intragastric gavage to rats exposed to chronic unpredictable mild stress (CUMS), and the antidepressive effects were investigated through behavioral tests, intestinal morphology and corticosterone levels.
31896054	3	63	theme	chronic	594:600	arg1	stress					621:626	chronic unpredictable mild stress	594:626	chronic unpredictable mild stress (CUMS)	594:633	STUDY DESIGN AND METHODS FOSs was introduced via intragastric gavage to rats exposed to chronic unpredictable mild stress (CUMS), and the antidepressive effects were investigated through behavioral tests, intestinal morphology and corticosterone levels.
31896054	3	64	theme	behavioral	693:702	arg1	tests					704:708	behavioral tests	693:708	behavioral tests	693:708	STUDY DESIGN AND METHODS FOSs was introduced via intragastric gavage to rats exposed to chronic unpredictable mild stress (CUMS), and the antidepressive effects were investigated through behavioral tests, intestinal morphology and corticosterone levels.
31896054	5	65	theme	intestinal	1082:1091	arg1	epithelia					1093:1101	repaired intestinal epithelia damages	1073:1109	repaired intestinal epithelia damages	1073:1109	RESULTS It was observed that FOSs alleviated depression-like behaviors and repaired intestinal epithelia damages.
31896054	1	66	contain	have	292:295	arg1	FOSs					180:183	FOSs	180:183	FOSs	180:183	BACKGROUND Inulin-type fructo-oligosaccharides (FOSs) purified from Morinda officinalis How., an effective oral antidepressant for mild to moderate depression, have a largely unknown efficacy and poor bioavailability.
31896054	1	66	contain	have	292:295	arg1	antidepressant					244:257	antidepressant	244:257	antidepressant	244:257	BACKGROUND Inulin-type fructo-oligosaccharides (FOSs) purified from Morinda officinalis How., an effective oral antidepressant for mild to moderate depression, have a largely unknown efficacy and poor bioavailability.
31896054	1	66	contain	have	292:295	arg1	fructo-oligosaccharides					155:177	BACKGROUND Inulin-type fructo-oligosaccharides	132:177	BACKGROUND Inulin-type fructo-oligosaccharides (FOSs) purified from Morinda officinalis How.	132:223	BACKGROUND Inulin-type fructo-oligosaccharides (FOSs) purified from Morinda officinalis How., an effective oral antidepressant for mild to moderate depression, have a largely unknown efficacy and poor bioavailability.
31896054	1	66	contain	have	292:295	arg2	efficacy					315:322	a largely unknown efficacy	297:322	a largely unknown efficacy	297:322	BACKGROUND Inulin-type fructo-oligosaccharides (FOSs) purified from Morinda officinalis How., an effective oral antidepressant for mild to moderate depression, have a largely unknown efficacy and poor bioavailability.
31896054	1	66	contain	have	292:295	arg2	bioavailability					333:347	poor bioavailability	328:347	poor bioavailability	328:347	BACKGROUND Inulin-type fructo-oligosaccharides (FOSs) purified from Morinda officinalis How., an effective oral antidepressant for mild to moderate depression, have a largely unknown efficacy and poor bioavailability.
31896054	1	67	theme	Inulin-type	143:153	arg1	FOSs					180:183	FOSs	180:183	FOSs	180:183	BACKGROUND Inulin-type fructo-oligosaccharides (FOSs) purified from Morinda officinalis How., an effective oral antidepressant for mild to moderate depression, have a largely unknown efficacy and poor bioavailability.
31896054	1	67	theme	Inulin-type	143:153	arg1	antidepressant					244:257	antidepressant	244:257	antidepressant	244:257	BACKGROUND Inulin-type fructo-oligosaccharides (FOSs) purified from Morinda officinalis How., an effective oral antidepressant for mild to moderate depression, have a largely unknown efficacy and poor bioavailability.
31896054	1	67	theme	Inulin-type	143:153	arg1	fructo-oligosaccharides					155:177	BACKGROUND Inulin-type fructo-oligosaccharides	132:177	BACKGROUND Inulin-type fructo-oligosaccharides (FOSs) purified from Morinda officinalis How.	132:223	BACKGROUND Inulin-type fructo-oligosaccharides (FOSs) purified from Morinda officinalis How., an effective oral antidepressant for mild to moderate depression, have a largely unknown efficacy and poor bioavailability.
31896054	4	68	theme	least	919:923	arg1	PLS-DA					956:961	PLS-DA	956:961	PLS-DA	956:961	Bacterial genomic DNA was extracted from feces, and the GM was profiled for using enterobacterial repetitive intergenic consensus (ERIC)-PCR analysis, partial least squares-discriminant analysis (PLS-DA) and 16S rRNA gene pyrosequencing.
31896054	4	68	theme	least	919:923	arg1	analysis					946:953	partial least squares-discriminant analysis	911:953	partial least squares-discriminant analysis (PLS-DA)	911:962	Bacterial genomic DNA was extracted from feces, and the GM was profiled for using enterobacterial repetitive intergenic consensus (ERIC)-PCR analysis, partial least squares-discriminant analysis (PLS-DA) and 16S rRNA gene pyrosequencing.
31896054	1	69	dep	moderate	271:278	arg1	to					268:269	to	268:269	to	268:269	BACKGROUND Inulin-type fructo-oligosaccharides (FOSs) purified from Morinda officinalis How., an effective oral antidepressant for mild to moderate depression, have a largely unknown efficacy and poor bioavailability.
31896054	10	70	theme	fluoxetine	2030:2039	arg1	component					1973:1981	a component	1971:1981	a component of FOSs, degrees of polymerization (DP) 5, and fluoxetine, the standard antidepressant drug	1971:2073	Apparently, FOSs-induced modulation of GM was more antidepressive compared to a component of FOSs, degrees of polymerization (DP) 5, and fluoxetine, the standard antidepressant drug.
31896054	0	71	theme	depression	89:98	arg1	features					100:107	depression features	89:107	depression features	89:107	Fructo-oligosaccharides from Morinda officinalis remodeled gut microbiota and alleviated depression features in a stress rat model.
31896054	11	72	theme	GM	2238:2239	arg1	modulation					2212:2221	the modulation	2208:2221	the modulation of the host' s GM	2208:2239	CONCLUSION In conclusion, this study implied that antidepressant efficacy of FOSs was inseparable from and strongly associated with the modulation of the host' s GM.
31896054	0	73	from	officinalis	37:47	arg1	Fructo-oligosaccharides					0:22	Fructo-oligosaccharides	0:22	Fructo-oligosaccharides from Morinda officinalis	0:47	Fructo-oligosaccharides from Morinda officinalis remodeled gut microbiota and alleviated depression features in a stress rat model.
31896054	7	74	theme	model	1244:1248	arg1	rats					1250:1253	normal and model rats	1233:1253	normal and model rats	1233:1253	Moreover, the GM compositions of normal and model rats were distantly clustered and were mainly related to the disappearance of beneficial bacteria (e.g., Acinetobacter, Barnesiella, Coprococcus, Dialister, Lactobacillus, and Paenibacillus) and appearance of depression-associated bacteria (e.g., Anaerostipes, Oscillibacter, Proteobacteria, and Streptococcus) in depressive rats.
31896054	10	75	theme	antidepressant	2055:2068	arg1	fluoxetine					2030:2039	fluoxetine	2030:2039	fluoxetine	2030:2039	Apparently, FOSs-induced modulation of GM was more antidepressive compared to a component of FOSs, degrees of polymerization (DP) 5, and fluoxetine, the standard antidepressant drug.
31896054	10	75	theme	antidepressant	2055:2068	arg1	drug					2070:2073	the standard antidepressant drug	2042:2073	the standard antidepressant drug	2042:2073	Apparently, FOSs-induced modulation of GM was more antidepressive compared to a component of FOSs, degrees of polymerization (DP) 5, and fluoxetine, the standard antidepressant drug.
31896054	0	76	theme	stress	114:119	arg1	model					125:129	a stress rat model	112:129	a stress rat model	112:129	Fructo-oligosaccharides from Morinda officinalis remodeled gut microbiota and alleviated depression features in a stress rat model.
31896054	9	77	theme	important	1812:1820	arg1	H2S					1843:1845	H2S	1843:1845	H2S	1843:1845	Notably, FOSs promoted the abundance of the bacterial phylum Cyanobacteria, a group of bacteria known for the secretion of pharmacologically important metabolites, such as H2S, that exhibit antidepressant-like properties.
31896054	9	77	theme	important	1812:1820	arg1	metabolites					1822:1832	pharmacologically important metabolites	1794:1832	pharmacologically important metabolites	1794:1832	Notably, FOSs promoted the abundance of the bacterial phylum Cyanobacteria, a group of bacteria known for the secretion of pharmacologically important metabolites, such as H2S, that exhibit antidepressant-like properties.
31896054	4	78	theme	rRNA	972:975	arg1	pyrosequencing					982:995	16S rRNA gene pyrosequencing	968:995	16S rRNA gene pyrosequencing	968:995	Bacterial genomic DNA was extracted from feces, and the GM was profiled for using enterobacterial repetitive intergenic consensus (ERIC)-PCR analysis, partial least squares-discriminant analysis (PLS-DA) and 16S rRNA gene pyrosequencing.
31896054	9	79	theme	metabolites	1822:1832	arg1	secretion					1781:1789	the secretion	1777:1789	the secretion of pharmacologically important metabolites, such as H2S, that exhibit antidepressant-like properties	1777:1890	Notably, FOSs promoted the abundance of the bacterial phylum Cyanobacteria, a group of bacteria known for the secretion of pharmacologically important metabolites, such as H2S, that exhibit antidepressant-like properties.
31896054	6	80	theme	FOSs	1112:1115	arg1	treatment					1117:1125	FOSs treatment	1112:1125	FOSs treatment	1112:1125	FOSs treatment lowered corticosterone levels in the plasma and urine of the model rats.
31896054	2	81	dep	PURPOSE	350:356	arg1	used					403:406	used	403:406	was used to investigate the antidepressive properties of FOSs at the interface of the gut microbiota (GM)	399:503	PURPOSE Therefore, the microbiota-gut-brain axis was used to investigate the antidepressive properties of FOSs at the interface of the gut microbiota (GM).
31896054	7	82	dep	Oscillibacter	1511:1523	arg1	e.g.					1491:1494	e.g.	1491:1494	e.g.	1491:1494	Moreover, the GM compositions of normal and model rats were distantly clustered and were mainly related to the disappearance of beneficial bacteria (e.g., Acinetobacter, Barnesiella, Coprococcus, Dialister, Lactobacillus, and Paenibacillus) and appearance of depression-associated bacteria (e.g., Anaerostipes, Oscillibacter, Proteobacteria, and Streptococcus) in depressive rats.
31896054	7	83	theme	bacteria	1339:1346	arg1	disappearance					1311:1323	the disappearance	1307:1323	the disappearance of beneficial bacteria (e.g., Acinetobacter, Barnesiella, Coprococcus, Dialister, Lactobacillus, and Paenibacillus) and appearance of depression-associated bacteria (e.g., Anaerostipes, Oscillibacter, Proteobacteria, and Streptococcus) in depressive rats	1307:1578	Moreover, the GM compositions of normal and model rats were distantly clustered and were mainly related to the disappearance of beneficial bacteria (e.g., Acinetobacter, Barnesiella, Coprococcus, Dialister, Lactobacillus, and Paenibacillus) and appearance of depression-associated bacteria (e.g., Anaerostipes, Oscillibacter, Proteobacteria, and Streptococcus) in depressive rats.
31896054	3	84	theme	antidepressive	644:657	arg1	effects					659:665	the antidepressive effects	640:665	the antidepressive effects	640:665	STUDY DESIGN AND METHODS FOSs was introduced via intragastric gavage to rats exposed to chronic unpredictable mild stress (CUMS), and the antidepressive effects were investigated through behavioral tests, intestinal morphology and corticosterone levels.
31896054	2	85	theme	FOSs	456:459	arg1	properties					442:451	the antidepressive properties	423:451	the antidepressive properties of FOSs	423:459	PURPOSE Therefore, the microbiota-gut-brain axis was used to investigate the antidepressive properties of FOSs at the interface of the gut microbiota (GM).
31896054	0	86	theme	gut	59:61	arg1	microbiota					63:72	gut microbiota	59:72	gut microbiota	59:72	Fructo-oligosaccharides from Morinda officinalis remodeled gut microbiota and alleviated depression features in a stress rat model.
31896054	8	87	theme	depressive	1613:1622	arg1	rats					1624:1627	depressive rats'	1613:1628	depressive rats' gut	1613:1632	Interestingly, the dysbiosis in depressive rats' gut was reinstated with FOSs treatments.
31896054	1	88	theme	poor	328:331	arg1	bioavailability					333:347	poor bioavailability	328:347	poor bioavailability	328:347	BACKGROUND Inulin-type fructo-oligosaccharides (FOSs) purified from Morinda officinalis How., an effective oral antidepressant for mild to moderate depression, have a largely unknown efficacy and poor bioavailability.
31896054	7	89	theme	depressive	1564:1573	arg1	rats					1575:1578	depressive rats	1564:1578	depressive rats	1564:1578	Moreover, the GM compositions of normal and model rats were distantly clustered and were mainly related to the disappearance of beneficial bacteria (e.g., Acinetobacter, Barnesiella, Coprococcus, Dialister, Lactobacillus, and Paenibacillus) and appearance of depression-associated bacteria (e.g., Anaerostipes, Oscillibacter, Proteobacteria, and Streptococcus) in depressive rats.
31896054	2	90	theme	gut	485:487	arg1	microbiota					489:498	the gut microbiota	481:498	the gut microbiota (GM)	481:503	PURPOSE Therefore, the microbiota-gut-brain axis was used to investigate the antidepressive properties of FOSs at the interface of the gut microbiota (GM).
31896054	2	90	theme	gut	485:487	arg1	GM					501:502	GM	501:502	GM	501:502	PURPOSE Therefore, the microbiota-gut-brain axis was used to investigate the antidepressive properties of FOSs at the interface of the gut microbiota (GM).
31896054	7	91	dep	bacteria	1339:1346	arg1	bacteria					1339:1346	beneficial bacteria	1328:1346	beneficial bacteria (e.g., Acinetobacter, Barnesiella, Coprococcus, Dialister, Lactobacillus, and Paenibacillus)	1328:1439	Moreover, the GM compositions of normal and model rats were distantly clustered and were mainly related to the disappearance of beneficial bacteria (e.g., Acinetobacter, Barnesiella, Coprococcus, Dialister, Lactobacillus, and Paenibacillus) and appearance of depression-associated bacteria (e.g., Anaerostipes, Oscillibacter, Proteobacteria, and Streptococcus) in depressive rats.
31896054	7	91	dep	bacteria	1339:1346	arg1	Barnesiella					1370:1380	Barnesiella	1370:1380	Barnesiella	1370:1380	Moreover, the GM compositions of normal and model rats were distantly clustered and were mainly related to the disappearance of beneficial bacteria (e.g., Acinetobacter, Barnesiella, Coprococcus, Dialister, Lactobacillus, and Paenibacillus) and appearance of depression-associated bacteria (e.g., Anaerostipes, Oscillibacter, Proteobacteria, and Streptococcus) in depressive rats.
31896054	7	91	dep	bacteria	1339:1346	arg1	Lactobacillus					1407:1419	Lactobacillus	1407:1419	Lactobacillus	1407:1419	Moreover, the GM compositions of normal and model rats were distantly clustered and were mainly related to the disappearance of beneficial bacteria (e.g., Acinetobacter, Barnesiella, Coprococcus, Dialister, Lactobacillus, and Paenibacillus) and appearance of depression-associated bacteria (e.g., Anaerostipes, Oscillibacter, Proteobacteria, and Streptococcus) in depressive rats.
31896054	7	91	dep	bacteria	1339:1346	arg1	Paenibacillus					1426:1438	Paenibacillus	1426:1438	Paenibacillus	1426:1438	Moreover, the GM compositions of normal and model rats were distantly clustered and were mainly related to the disappearance of beneficial bacteria (e.g., Acinetobacter, Barnesiella, Coprococcus, Dialister, Lactobacillus, and Paenibacillus) and appearance of depression-associated bacteria (e.g., Anaerostipes, Oscillibacter, Proteobacteria, and Streptococcus) in depressive rats.
31896054	7	91	dep	bacteria	1339:1346	arg1	Dialister					1396:1404	Dialister	1396:1404	Dialister	1396:1404	Moreover, the GM compositions of normal and model rats were distantly clustered and were mainly related to the disappearance of beneficial bacteria (e.g., Acinetobacter, Barnesiella, Coprococcus, Dialister, Lactobacillus, and Paenibacillus) and appearance of depression-associated bacteria (e.g., Anaerostipes, Oscillibacter, Proteobacteria, and Streptococcus) in depressive rats.
31896054	11	92	dep	CONCLUSION	2076:2085	arg1	conclusion					2090:2099	conclusion	2090:2099	conclusion	2090:2099	CONCLUSION In conclusion, this study implied that antidepressant efficacy of FOSs was inseparable from and strongly associated with the modulation of the host' s GM.
31896054	3	93	theme	intestinal	711:720	arg1	morphology					722:731	intestinal morphology	711:731	intestinal morphology	711:731	STUDY DESIGN AND METHODS FOSs was introduced via intragastric gavage to rats exposed to chronic unpredictable mild stress (CUMS), and the antidepressive effects were investigated through behavioral tests, intestinal morphology and corticosterone levels.
30588801	0	0	theme	Gas	98:100	arg1	Spectrometry					122:133	Pyrolysis Gas Chromatography-Mass Spectrometry	88:133	Pyrolysis Gas Chromatography-Mass Spectrometry	88:133	Cloud-Point Extraction Combined with Thermal Degradation for Nanoplastic Analysis Using Pyrolysis Gas Chromatography-Mass Spectrometry.
30588801	7	1	theme	86.2	1181:1184	arg1	nm					1186:1187	nm	1186:1187	nm	1186:1187	Taking 66.2 nm PS nanoplastics and 86.2 nm PMMA nanoplastics as examples, the proposed method showed excellent reproducibility, and high sensitivity with respective detection limits of 11.5 and 2.5 fM.
30588801	1	2	theme	marine	184:189	arg1	systems					191:197	marine systems	184:197	marine systems	184:197	The contamination of micro- and nanoplastics in marine systems and freshwater is a global issue.
30588801	9	3	theme	spiked	1498:1503	arg1	level					1505:1509	a spiked level	1496:1509	a spiked level of 88.6 fM for PS nanoplastics	1496:1540	Recoveries of 84.6-96.6% at a spiked level of 88.6 fM for PS nanoplastics and 76.5-96.6% at a spiked level of 50.4 fM for PMMA nanoplastics were obtained.
30588801	4	4	theme	-based	567:572	arg1	CPE					598:600	CPE	598:600	CPE	598:600	Herein, for the first time, a Triton X-45 (TX-45)-based cloud-point extraction (CPE) was proposed to preconcentrate trace nanoplastics in environmental waters.
30588801	4	4	theme	-based	567:572	arg1	extraction					586:595	a Triton X-45 (TX-45)-based cloud-point extraction	546:595	a Triton X-45 (TX-45)-based cloud-point extraction (CPE)	546:601	Herein, for the first time, a Triton X-45 (TX-45)-based cloud-point extraction (CPE) was proposed to preconcentrate trace nanoplastics in environmental waters.
30588801	0	5	theme	Pyrolysis	88:96	arg1	Spectrometry					122:133	Pyrolysis Gas Chromatography-Mass Spectrometry	88:133	Pyrolysis Gas Chromatography-Mass Spectrometry	88:133	Cloud-Point Extraction Combined with Thermal Degradation for Nanoplastic Analysis Using Pyrolysis Gas Chromatography-Mass Spectrometry.
30588801	6	6	from	°C	979:980	arg1	treatment					962:970	thermal treatment	954:970	thermal treatment at 190 °C for 3 h	954:988	Additionally, following thermal treatment at 190 °C for 3 h, the CPE-obtained extract could be submitted to pyrolysis gas chromatography-mass spectrometry (Py-GC/MS) analysis for mass quantification of nanoplastics.
30588801	6	7	theme	pyrolysis	1038:1046	arg1	Py-GC/MS					1086:1093	Py-GC/MS	1086:1093	Py-GC/MS	1086:1093	Additionally, following thermal treatment at 190 °C for 3 h, the CPE-obtained extract could be submitted to pyrolysis gas chromatography-mass spectrometry (Py-GC/MS) analysis for mass quantification of nanoplastics.
30588801	6	7	theme	pyrolysis	1038:1046	arg1	spectrometry					1072:1083	pyrolysis gas chromatography-mass spectrometry	1038:1083	pyrolysis gas chromatography-mass spectrometry (Py-GC/MS) analysis	1038:1103	Additionally, following thermal treatment at 190 °C for 3 h, the CPE-obtained extract could be submitted to pyrolysis gas chromatography-mass spectrometry (Py-GC/MS) analysis for mass quantification of nanoplastics.
30588801	5	8	theme	enrichment	722:731	arg1	factor					733:738	an enrichment factor	719:738	an enrichment factor of 500	719:745	Under the optimum extraction conditions, an enrichment factor of 500 was obtained for two types of nanoplastics with different compositions, polystyrene (PS) and poly(methyl methacrylate) (PMMA), without disturbing their original morphology and sizes.
30588801	7	9	theme	respective	1300:1309	arg1	limits					1321:1326	respective detection limits	1300:1326	respective detection limits of 11.5 and 2.5 fM	1300:1345	Taking 66.2 nm PS nanoplastics and 86.2 nm PMMA nanoplastics as examples, the proposed method showed excellent reproducibility, and high sensitivity with respective detection limits of 11.5 and 2.5 fM.
30588801	8	10	theme	approach	1376:1383	arg1	Feasibility					1348:1358	Feasibility	1348:1358	Feasibility of the proposed approach	1348:1383	Feasibility of the proposed approach was verified by application of the optimized procedure to four real water samples.
30588801	5	11	with	nanoplastics	777:788	arg1	compositions					805:816	different compositions	795:816	different compositions	795:816	Under the optimum extraction conditions, an enrichment factor of 500 was obtained for two types of nanoplastics with different compositions, polystyrene (PS) and poly(methyl methacrylate) (PMMA), without disturbing their original morphology and sizes.
30588801	9	12	from	level	1505:1509	arg1	Recoveries					1468:1477	Recoveries	1468:1477	Recoveries of 84.6-96.6% at a spiked level of 88.6 fM for PS nanoplastics and 76.5-96.6% at a spiked level of 50.4 fM for PMMA nanoplastics	1468:1606	Recoveries of 84.6-96.6% at a spiked level of 88.6 fM for PS nanoplastics and 76.5-96.6% at a spiked level of 50.4 fM for PMMA nanoplastics were obtained.
30588801	9	12	from	level	1505:1509	arg1	%					1491:1491	84.6-96.6%	1482:1491	84.6-96.6% at a spiked level of 88.6 fM for PS nanoplastics	1482:1540	Recoveries of 84.6-96.6% at a spiked level of 88.6 fM for PS nanoplastics and 76.5-96.6% at a spiked level of 50.4 fM for PMMA nanoplastics were obtained.
30588801	9	12	from	level	1505:1509	arg1	%					1555:1555	76.5-96.6%	1546:1555	76.5-96.6% at a spiked level of 50.4 fM	1546:1584	Recoveries of 84.6-96.6% at a spiked level of 88.6 fM for PS nanoplastics and 76.5-96.6% at a spiked level of 50.4 fM for PMMA nanoplastics were obtained.
30588801	0	13	theme	Chromatography-Mass	102:120	arg1	Spectrometry					122:133	Pyrolysis Gas Chromatography-Mass Spectrometry	88:133	Pyrolysis Gas Chromatography-Mass Spectrometry	88:133	Cloud-Point Extraction Combined with Thermal Degradation for Nanoplastic Analysis Using Pyrolysis Gas Chromatography-Mass Spectrometry.
30588801	6	14	theme	thermal	954:960	arg1	treatment					962:970	thermal treatment	954:970	thermal treatment at 190 °C for 3 h	954:988	Additionally, following thermal treatment at 190 °C for 3 h, the CPE-obtained extract could be submitted to pyrolysis gas chromatography-mass spectrometry (Py-GC/MS) analysis for mass quantification of nanoplastics.
30588801	2	15	theme	high	307:310	arg1	priority					312:319	high priority	307:319	high priority	307:319	Determination of micro- and nanoplastics in the aqueous environment is of high priority to fully assess the risk that plastic particles will pose.
30588801	9	16	from	level	1569:1573	arg1	Recoveries					1468:1477	Recoveries	1468:1477	Recoveries of 84.6-96.6% at a spiked level of 88.6 fM for PS nanoplastics and 76.5-96.6% at a spiked level of 50.4 fM for PMMA nanoplastics	1468:1606	Recoveries of 84.6-96.6% at a spiked level of 88.6 fM for PS nanoplastics and 76.5-96.6% at a spiked level of 50.4 fM for PMMA nanoplastics were obtained.
30588801	9	16	from	level	1569:1573	arg1	%					1491:1491	84.6-96.6%	1482:1491	84.6-96.6% at a spiked level of 88.6 fM for PS nanoplastics	1482:1540	Recoveries of 84.6-96.6% at a spiked level of 88.6 fM for PS nanoplastics and 76.5-96.6% at a spiked level of 50.4 fM for PMMA nanoplastics were obtained.
30588801	9	16	from	level	1569:1573	arg1	%					1555:1555	76.5-96.6%	1546:1555	76.5-96.6% at a spiked level of 50.4 fM	1546:1584	Recoveries of 84.6-96.6% at a spiked level of 88.6 fM for PS nanoplastics and 76.5-96.6% at a spiked level of 50.4 fM for PMMA nanoplastics were obtained.
30588801	4	17	theme	cloud-point	574:584	arg1	CPE					598:600	CPE	598:600	CPE	598:600	Herein, for the first time, a Triton X-45 (TX-45)-based cloud-point extraction (CPE) was proposed to preconcentrate trace nanoplastics in environmental waters.
30588801	4	17	theme	cloud-point	574:584	arg1	extraction					586:595	a Triton X-45 (TX-45)-based cloud-point extraction	546:595	a Triton X-45 (TX-45)-based cloud-point extraction (CPE)	546:601	Herein, for the first time, a Triton X-45 (TX-45)-based cloud-point extraction (CPE) was proposed to preconcentrate trace nanoplastics in environmental waters.
30588801	2	18	from	Determination	233:245	arg1	environment					289:299	the aqueous environment	277:299	the aqueous environment	277:299	Determination of micro- and nanoplastics in the aqueous environment is of high priority to fully assess the risk that plastic particles will pose.
30588801	6	19	theme	gas	1048:1050	arg1	Py-GC/MS					1086:1093	Py-GC/MS	1086:1093	Py-GC/MS	1086:1093	Additionally, following thermal treatment at 190 °C for 3 h, the CPE-obtained extract could be submitted to pyrolysis gas chromatography-mass spectrometry (Py-GC/MS) analysis for mass quantification of nanoplastics.
30588801	6	19	theme	gas	1048:1050	arg1	spectrometry					1072:1083	pyrolysis gas chromatography-mass spectrometry	1038:1083	pyrolysis gas chromatography-mass spectrometry (Py-GC/MS) analysis	1038:1103	Additionally, following thermal treatment at 190 °C for 3 h, the CPE-obtained extract could be submitted to pyrolysis gas chromatography-mass spectrometry (Py-GC/MS) analysis for mass quantification of nanoplastics.
30588801	10	20	theme	nanoplastic	1682:1692	arg1	analysis					1694:1701	nanoplastic analysis	1682:1701	nanoplastic analysis in environmental waters	1682:1725	Consequently, this work provides an efficient approach for nanoplastic analysis in environmental waters.
30588801	5	21	theme	methyl	845:850	arg1	poly					840:843	poly	840:843	poly(methyl methacrylate) (PMMA)	840:871	Under the optimum extraction conditions, an enrichment factor of 500 was obtained for two types of nanoplastics with different compositions, polystyrene (PS) and poly(methyl methacrylate) (PMMA), without disturbing their original morphology and sizes.
30588801	5	21	theme	methyl	845:850	arg1	methacrylate					852:863	methyl methacrylate	845:863	methyl methacrylate	845:863	Under the optimum extraction conditions, an enrichment factor of 500 was obtained for two types of nanoplastics with different compositions, polystyrene (PS) and poly(methyl methacrylate) (PMMA), without disturbing their original morphology and sizes.
30588801	6	22	theme	CPE-obtained	995:1006	arg1	extract					1008:1014	the CPE-obtained extract	991:1014	the CPE-obtained extract	991:1014	Additionally, following thermal treatment at 190 °C for 3 h, the CPE-obtained extract could be submitted to pyrolysis gas chromatography-mass spectrometry (Py-GC/MS) analysis for mass quantification of nanoplastics.
30588801	5	23	theme	500	743:745	arg1	factor					733:738	an enrichment factor	719:738	an enrichment factor of 500	719:745	Under the optimum extraction conditions, an enrichment factor of 500 was obtained for two types of nanoplastics with different compositions, polystyrene (PS) and poly(methyl methacrylate) (PMMA), without disturbing their original morphology and sizes.
30588801	7	24	theme	high	1278:1281	arg1	sensitivity					1283:1293	high sensitivity	1278:1293	high sensitivity	1278:1293	Taking 66.2 nm PS nanoplastics and 86.2 nm PMMA nanoplastics as examples, the proposed method showed excellent reproducibility, and high sensitivity with respective detection limits of 11.5 and 2.5 fM.
30588801	6	25	theme	nanoplastics	1132:1143	arg1	quantification					1114:1127	mass quantification	1109:1127	mass quantification of nanoplastics	1109:1143	Additionally, following thermal treatment at 190 °C for 3 h, the CPE-obtained extract could be submitted to pyrolysis gas chromatography-mass spectrometry (Py-GC/MS) analysis for mass quantification of nanoplastics.
30588801	7	26	theme	detection	1311:1319	arg1	limits					1321:1326	respective detection limits	1300:1326	respective detection limits of 11.5 and 2.5 fM	1300:1345	Taking 66.2 nm PS nanoplastics and 86.2 nm PMMA nanoplastics as examples, the proposed method showed excellent reproducibility, and high sensitivity with respective detection limits of 11.5 and 2.5 fM.
30588801	5	27	theme	different	795:803	arg1	compositions					805:816	different compositions	795:816	different compositions	795:816	Under the optimum extraction conditions, an enrichment factor of 500 was obtained for two types of nanoplastics with different compositions, polystyrene (PS) and poly(methyl methacrylate) (PMMA), without disturbing their original morphology and sizes.
30588801	7	28	theme	nm	1186:1187	arg1	PMMA					1189:1192	86.2 nm PMMA	1181:1192	86.2 nm PMMA	1181:1192	Taking 66.2 nm PS nanoplastics and 86.2 nm PMMA nanoplastics as examples, the proposed method showed excellent reproducibility, and high sensitivity with respective detection limits of 11.5 and 2.5 fM.
30588801	9	29	theme	fM	1583:1584	arg1	level					1569:1573	a spiked level	1560:1573	a spiked level of 50.4 fM	1560:1584	Recoveries of 84.6-96.6% at a spiked level of 88.6 fM for PS nanoplastics and 76.5-96.6% at a spiked level of 50.4 fM for PMMA nanoplastics were obtained.
30588801	5	30	theme	original	899:906	arg1	morphology					908:917	their original morphology	893:917	their original morphology	893:917	Under the optimum extraction conditions, an enrichment factor of 500 was obtained for two types of nanoplastics with different compositions, polystyrene (PS) and poly(methyl methacrylate) (PMMA), without disturbing their original morphology and sizes.
30588801	4	31	from	nanoplastics	640:651	arg1	waters					670:675	environmental waters	656:675	environmental waters	656:675	Herein, for the first time, a Triton X-45 (TX-45)-based cloud-point extraction (CPE) was proposed to preconcentrate trace nanoplastics in environmental waters.
30588801	3	32	theme	aquatic	438:444	arg1	ecosystems					446:455	aquatic ecosystems	438:455	aquatic ecosystems	438:455	Although microplastics have been detected in a variety of aquatic ecosystems, the analysis of nanoplastics remains an unsolved challenge.
30588801	0	33	theme	Cloud-Point	0:10	arg1	Extraction					12:21	Cloud-Point Extraction	0:21	Cloud-Point Extraction	0:21	Cloud-Point Extraction Combined with Thermal Degradation for Nanoplastic Analysis Using Pyrolysis Gas Chromatography-Mass Spectrometry.
30588801	9	34	theme	fM	1519:1520	arg1	level					1505:1509	a spiked level	1496:1509	a spiked level of 88.6 fM for PS nanoplastics	1496:1540	Recoveries of 84.6-96.6% at a spiked level of 88.6 fM for PS nanoplastics and 76.5-96.6% at a spiked level of 50.4 fM for PMMA nanoplastics were obtained.
30588801	9	35	theme	spiked	1562:1567	arg1	level					1569:1573	a spiked level	1560:1573	a spiked level of 50.4 fM	1560:1584	Recoveries of 84.6-96.6% at a spiked level of 88.6 fM for PS nanoplastics and 76.5-96.6% at a spiked level of 50.4 fM for PMMA nanoplastics were obtained.
30588801	1	36	from	contamination	140:152	arg1	freshwater					203:212	freshwater	203:212	freshwater	203:212	The contamination of micro- and nanoplastics in marine systems and freshwater is a global issue.
30588801	1	36	from	contamination	140:152	arg1	systems					191:197	marine systems	184:197	marine systems	184:197	The contamination of micro- and nanoplastics in marine systems and freshwater is a global issue.
30588801	3	37	theme	ecosystems	446:455	arg1	variety					427:433	a variety	425:433	a variety of aquatic ecosystems	425:455	Although microplastics have been detected in a variety of aquatic ecosystems, the analysis of nanoplastics remains an unsolved challenge.
30588801	3	37	theme	ecosystems	446:455	arg1	ecosystems					446:455	aquatic ecosystems	438:455	aquatic ecosystems	438:455	Although microplastics have been detected in a variety of aquatic ecosystems, the analysis of nanoplastics remains an unsolved challenge.
30588801	5	38	theme	extraction	696:705	arg1	conditions					707:716	the optimum extraction conditions	684:716	the optimum extraction conditions	684:716	Under the optimum extraction conditions, an enrichment factor of 500 was obtained for two types of nanoplastics with different compositions, polystyrene (PS) and poly(methyl methacrylate) (PMMA), without disturbing their original morphology and sizes.
30588801	8	39	theme	proposed	1367:1374	arg1	approach					1376:1383	the proposed approach	1363:1383	the proposed approach	1363:1383	Feasibility of the proposed approach was verified by application of the optimized procedure to four real water samples.
30588801	6	40	theme	mass	1109:1112	arg1	quantification					1114:1127	mass quantification	1109:1127	mass quantification of nanoplastics	1109:1143	Additionally, following thermal treatment at 190 °C for 3 h, the CPE-obtained extract could be submitted to pyrolysis gas chromatography-mass spectrometry (Py-GC/MS) analysis for mass quantification of nanoplastics.
30588801	1	41	theme	global	219:224	arg1	issue					226:230	a global issue	217:230	a global issue	217:230	The contamination of micro- and nanoplastics in marine systems and freshwater is a global issue.
30588801	1	41	theme	global	219:224	arg1	contamination					140:152	The contamination	136:152	The contamination of micro- and nanoplastics in marine systems and freshwater	136:212	The contamination of micro- and nanoplastics in marine systems and freshwater is a global issue.
30588801	10	42	theme	environmental	1706:1718	arg1	waters					1720:1725	environmental waters	1706:1725	environmental waters	1706:1725	Consequently, this work provides an efficient approach for nanoplastic analysis in environmental waters.
30588801	6	43	theme	spectrometry	1072:1083	arg1	analysis					1096:1103	pyrolysis gas chromatography-mass spectrometry (Py-GC/MS) analysis	1038:1103	pyrolysis gas chromatography-mass spectrometry (Py-GC/MS) analysis	1038:1103	Additionally, following thermal treatment at 190 °C for 3 h, the CPE-obtained extract could be submitted to pyrolysis gas chromatography-mass spectrometry (Py-GC/MS) analysis for mass quantification of nanoplastics.
30588801	7	44	theme	nanoplastics	1164:1175	arg1	examples					1210:1217	examples	1210:1217	examples	1210:1217	Taking 66.2 nm PS nanoplastics and 86.2 nm PMMA nanoplastics as examples, the proposed method showed excellent reproducibility, and high sensitivity with respective detection limits of 11.5 and 2.5 fM.
30588801	7	44	theme	nanoplastics	1164:1175	arg1	nanoplastics					1194:1205	66.2 nm PS nanoplastics and 86.2 nm PMMA nanoplastics	1153:1205	66.2 nm PS nanoplastics and 86.2 nm PMMA nanoplastics	1153:1205	Taking 66.2 nm PS nanoplastics and 86.2 nm PMMA nanoplastics as examples, the proposed method showed excellent reproducibility, and high sensitivity with respective detection limits of 11.5 and 2.5 fM.
30588801	7	45	theme	excellent	1247:1255	arg1	reproducibility					1257:1271	excellent reproducibility	1247:1271	excellent reproducibility	1247:1271	Taking 66.2 nm PS nanoplastics and 86.2 nm PMMA nanoplastics as examples, the proposed method showed excellent reproducibility, and high sensitivity with respective detection limits of 11.5 and 2.5 fM.
30588801	7	46	theme	66.2	1153:1156	arg1	nm					1158:1159	nm	1158:1159	nm	1158:1159	Taking 66.2 nm PS nanoplastics and 86.2 nm PMMA nanoplastics as examples, the proposed method showed excellent reproducibility, and high sensitivity with respective detection limits of 11.5 and 2.5 fM.
30588801	2	47	theme	nanoplastics	261:272	arg1	Determination					233:245	Determination	233:245	Determination of micro- and nanoplastics in the aqueous environment	233:299	Determination of micro- and nanoplastics in the aqueous environment is of high priority to fully assess the risk that plastic particles will pose.
30588801	4	48	theme	environmental	656:668	arg1	waters					670:675	environmental waters	656:675	environmental waters	656:675	Herein, for the first time, a Triton X-45 (TX-45)-based cloud-point extraction (CPE) was proposed to preconcentrate trace nanoplastics in environmental waters.
30588801	4	49	theme	trace	634:638	arg1	nanoplastics					640:651	trace nanoplastics	634:651	trace nanoplastics in environmental waters	634:675	Herein, for the first time, a Triton X-45 (TX-45)-based cloud-point extraction (CPE) was proposed to preconcentrate trace nanoplastics in environmental waters.
30588801	0	50	theme	Thermal	37:43	arg1	Degradation					45:55	Thermal Degradation	37:55	Thermal Degradation	37:55	Cloud-Point Extraction Combined with Thermal Degradation for Nanoplastic Analysis Using Pyrolysis Gas Chromatography-Mass Spectrometry.
30588801	8	51	theme	procedure	1430:1438	arg1	application					1401:1411	application	1401:1411	application of the optimized procedure to four real water samples	1401:1465	Feasibility of the proposed approach was verified by application of the optimized procedure to four real water samples.
30588801	7	52	theme	PS	1161:1162	arg1	nanoplastics					1164:1175	66.2 nm PS nanoplastics	1153:1175	66.2 nm PS nanoplastics	1153:1175	Taking 66.2 nm PS nanoplastics and 86.2 nm PMMA nanoplastics as examples, the proposed method showed excellent reproducibility, and high sensitivity with respective detection limits of 11.5 and 2.5 fM.
30588801	0	53	theme	Nanoplastic	61:71	arg1	Analysis					73:80	Nanoplastic Analysis	61:80	Nanoplastic Analysis Using Pyrolysis Gas Chromatography-Mass Spectrometry	61:133	Cloud-Point Extraction Combined with Thermal Degradation for Nanoplastic Analysis Using Pyrolysis Gas Chromatography-Mass Spectrometry.
30588801	4	54	theme	first	534:538	arg1	time					540:543	the first time	530:543	the first time	530:543	Herein, for the first time, a Triton X-45 (TX-45)-based cloud-point extraction (CPE) was proposed to preconcentrate trace nanoplastics in environmental waters.
30588801	8	55	theme	optimized	1420:1428	arg1	procedure					1430:1438	the optimized procedure	1416:1438	the optimized procedure	1416:1438	Feasibility of the proposed approach was verified by application of the optimized procedure to four real water samples.
30588801	2	56	theme	micro-	250:255	arg1	Determination					233:245	Determination	233:245	Determination of micro- and nanoplastics in the aqueous environment	233:299	Determination of micro- and nanoplastics in the aqueous environment is of high priority to fully assess the risk that plastic particles will pose.
30588801	10	57	theme	efficient	1659:1667	arg1	approach					1669:1676	an efficient approach	1656:1676	an efficient approach for nanoplastic analysis in environmental waters	1656:1725	Consequently, this work provides an efficient approach for nanoplastic analysis in environmental waters.
30588801	3	58	located	detected	413:420	arg2	microplastics					389:401	microplastics	389:401	microplastics	389:401	Although microplastics have been detected in a variety of aquatic ecosystems, the analysis of nanoplastics remains an unsolved challenge.
30588801	3	58	located	detected	413:420	arg1	variety					427:433	a variety	425:433	a variety of aquatic ecosystems	425:455	Although microplastics have been detected in a variety of aquatic ecosystems, the analysis of nanoplastics remains an unsolved challenge.
30588801	3	58	located	detected	413:420	arg1	ecosystems					446:455	aquatic ecosystems	438:455	aquatic ecosystems	438:455	Although microplastics have been detected in a variety of aquatic ecosystems, the analysis of nanoplastics remains an unsolved challenge.
30588801	7	59	theme	fM	1344:1345	arg1	limits					1321:1326	respective detection limits	1300:1326	respective detection limits of 11.5 and 2.5 fM	1300:1345	Taking 66.2 nm PS nanoplastics and 86.2 nm PMMA nanoplastics as examples, the proposed method showed excellent reproducibility, and high sensitivity with respective detection limits of 11.5 and 2.5 fM.
30588801	9	60	theme	PMMA	1590:1593	arg1	nanoplastics					1595:1606	PMMA nanoplastics	1590:1606	PMMA nanoplastics	1590:1606	Recoveries of 84.6-96.6% at a spiked level of 88.6 fM for PS nanoplastics and 76.5-96.6% at a spiked level of 50.4 fM for PMMA nanoplastics were obtained.
30588801	2	61	theme	plastic	351:357	arg1	particles					359:367	plastic particles	351:367	plastic particles	351:367	Determination of micro- and nanoplastics in the aqueous environment is of high priority to fully assess the risk that plastic particles will pose.
30588801	9	62	theme	%	1555:1555	arg1	Recoveries					1468:1477	Recoveries	1468:1477	Recoveries of 84.6-96.6% at a spiked level of 88.6 fM for PS nanoplastics and 76.5-96.6% at a spiked level of 50.4 fM for PMMA nanoplastics	1468:1606	Recoveries of 84.6-96.6% at a spiked level of 88.6 fM for PS nanoplastics and 76.5-96.6% at a spiked level of 50.4 fM for PMMA nanoplastics were obtained.
30588801	9	63	from	Recoveries	1468:1477	arg1	level					1569:1573	a spiked level	1560:1573	a spiked level of 50.4 fM	1560:1584	Recoveries of 84.6-96.6% at a spiked level of 88.6 fM for PS nanoplastics and 76.5-96.6% at a spiked level of 50.4 fM for PMMA nanoplastics were obtained.
30588801	9	63	from	Recoveries	1468:1477	arg1	level					1505:1509	a spiked level	1496:1509	a spiked level of 88.6 fM for PS nanoplastics	1496:1540	Recoveries of 84.6-96.6% at a spiked level of 88.6 fM for PS nanoplastics and 76.5-96.6% at a spiked level of 50.4 fM for PMMA nanoplastics were obtained.
30588801	9	64	theme	%	1491:1491	arg1	Recoveries					1468:1477	Recoveries	1468:1477	Recoveries of 84.6-96.6% at a spiked level of 88.6 fM for PS nanoplastics and 76.5-96.6% at a spiked level of 50.4 fM for PMMA nanoplastics	1468:1606	Recoveries of 84.6-96.6% at a spiked level of 88.6 fM for PS nanoplastics and 76.5-96.6% at a spiked level of 50.4 fM for PMMA nanoplastics were obtained.
30588801	1	65	theme	micro-	157:162	arg1	issue					226:230	a global issue	217:230	a global issue	217:230	The contamination of micro- and nanoplastics in marine systems and freshwater is a global issue.
30588801	1	65	theme	micro-	157:162	arg1	contamination					140:152	The contamination	136:152	The contamination of micro- and nanoplastics in marine systems and freshwater	136:212	The contamination of micro- and nanoplastics in marine systems and freshwater is a global issue.
30588801	9	66	theme	PS	1526:1527	arg1	nanoplastics					1529:1540	PS nanoplastics	1526:1540	PS nanoplastics	1526:1540	Recoveries of 84.6-96.6% at a spiked level of 88.6 fM for PS nanoplastics and 76.5-96.6% at a spiked level of 50.4 fM for PMMA nanoplastics were obtained.
30588801	5	67	theme	optimum	688:694	arg1	conditions					707:716	the optimum extraction conditions	684:716	the optimum extraction conditions	684:716	Under the optimum extraction conditions, an enrichment factor of 500 was obtained for two types of nanoplastics with different compositions, polystyrene (PS) and poly(methyl methacrylate) (PMMA), without disturbing their original morphology and sizes.
30588801	8	68	theme	water	1453:1457	arg1	samples					1459:1465	four real water samples	1443:1465	four real water samples	1443:1465	Feasibility of the proposed approach was verified by application of the optimized procedure to four real water samples.
30588801	2	69	theme	aqueous	281:287	arg1	environment					289:299	the aqueous environment	277:299	the aqueous environment	277:299	Determination of micro- and nanoplastics in the aqueous environment is of high priority to fully assess the risk that plastic particles will pose.
30588801	3	70	theme	unsolved	498:505	arg1	challenge					507:515	an unsolved challenge	495:515	an unsolved challenge	495:515	Although microplastics have been detected in a variety of aquatic ecosystems, the analysis of nanoplastics remains an unsolved challenge.
30588801	7	71	theme	proposed	1224:1231	arg1	method					1233:1238	the proposed method	1220:1238	the proposed method	1220:1238	Taking 66.2 nm PS nanoplastics and 86.2 nm PMMA nanoplastics as examples, the proposed method showed excellent reproducibility, and high sensitivity with respective detection limits of 11.5 and 2.5 fM.
30588801	7	72	theme	PMMA	1189:1192	arg1	examples					1210:1217	examples	1210:1217	examples	1210:1217	Taking 66.2 nm PS nanoplastics and 86.2 nm PMMA nanoplastics as examples, the proposed method showed excellent reproducibility, and high sensitivity with respective detection limits of 11.5 and 2.5 fM.
30588801	7	72	theme	PMMA	1189:1192	arg1	nanoplastics					1194:1205	66.2 nm PS nanoplastics and 86.2 nm PMMA nanoplastics	1153:1205	66.2 nm PS nanoplastics and 86.2 nm PMMA nanoplastics	1153:1205	Taking 66.2 nm PS nanoplastics and 86.2 nm PMMA nanoplastics as examples, the proposed method showed excellent reproducibility, and high sensitivity with respective detection limits of 11.5 and 2.5 fM.
30588801	8	73	theme	real	1448:1451	arg1	samples					1459:1465	four real water samples	1443:1465	four real water samples	1443:1465	Feasibility of the proposed approach was verified by application of the optimized procedure to four real water samples.
30588801	3	74	theme	nanoplastics	474:485	arg1	analysis					462:469	the analysis	458:469	the analysis of nanoplastics	458:485	Although microplastics have been detected in a variety of aquatic ecosystems, the analysis of nanoplastics remains an unsolved challenge.
30588801	1	75	theme	nanoplastics	168:179	arg1	issue					226:230	a global issue	217:230	a global issue	217:230	The contamination of micro- and nanoplastics in marine systems and freshwater is a global issue.
30588801	1	75	theme	nanoplastics	168:179	arg1	contamination					140:152	The contamination	136:152	The contamination of micro- and nanoplastics in marine systems and freshwater	136:212	The contamination of micro- and nanoplastics in marine systems and freshwater is a global issue.
30588801	10	76	from	analysis	1694:1701	arg1	waters					1720:1725	environmental waters	1706:1725	environmental waters	1706:1725	Consequently, this work provides an efficient approach for nanoplastic analysis in environmental waters.
30588801	5	77	theme	nanoplastics	777:788	arg1	types					768:772	two types	764:772	two types of nanoplastics with different compositions	764:816	Under the optimum extraction conditions, an enrichment factor of 500 was obtained for two types of nanoplastics with different compositions, polystyrene (PS) and poly(methyl methacrylate) (PMMA), without disturbing their original morphology and sizes.
30588801	5	77	theme	nanoplastics	777:788	arg1	poly					840:843	poly	840:843	poly(methyl methacrylate) (PMMA)	840:871	Under the optimum extraction conditions, an enrichment factor of 500 was obtained for two types of nanoplastics with different compositions, polystyrene (PS) and poly(methyl methacrylate) (PMMA), without disturbing their original morphology and sizes.
30588801	5	77	theme	nanoplastics	777:788	arg1	polystyrene					819:829	polystyrene	819:829	polystyrene (PS)	819:834	Under the optimum extraction conditions, an enrichment factor of 500 was obtained for two types of nanoplastics with different compositions, polystyrene (PS) and poly(methyl methacrylate) (PMMA), without disturbing their original morphology and sizes.
30588801	7	78	theme	nm	1158:1159	arg1	nanoplastics					1164:1175	66.2 nm PS nanoplastics	1153:1175	66.2 nm PS nanoplastics	1153:1175	Taking 66.2 nm PS nanoplastics and 86.2 nm PMMA nanoplastics as examples, the proposed method showed excellent reproducibility, and high sensitivity with respective detection limits of 11.5 and 2.5 fM.
30588801	6	79	theme	chromatography-mass	1052:1070	arg1	Py-GC/MS					1086:1093	Py-GC/MS	1086:1093	Py-GC/MS	1086:1093	Additionally, following thermal treatment at 190 °C for 3 h, the CPE-obtained extract could be submitted to pyrolysis gas chromatography-mass spectrometry (Py-GC/MS) analysis for mass quantification of nanoplastics.
30588801	6	79	theme	chromatography-mass	1052:1070	arg1	spectrometry					1072:1083	pyrolysis gas chromatography-mass spectrometry	1038:1083	pyrolysis gas chromatography-mass spectrometry (Py-GC/MS) analysis	1038:1103	Additionally, following thermal treatment at 190 °C for 3 h, the CPE-obtained extract could be submitted to pyrolysis gas chromatography-mass spectrometry (Py-GC/MS) analysis for mass quantification of nanoplastics.
30763656	0	0	theme	olanzapine-mediated	69:87	arg1	gain					96:99	olanzapine-mediated weight gain	69:99	olanzapine-mediated weight gain in rats	69:107	Prebiotic reduction of brain histone deacetylase (HDAC) activity and olanzapine-mediated weight gain in rats, are acetate independent.
30763656	2	1	theme	weight	503:508	arg1	gain					510:513	olanzapine-induced weight gain	484:513	olanzapine-induced weight gain	484:513	Ingestion of the prebiotic Bimuno™ galacto-oligosaccharide (B-GOS®) is one such intervention that has been shown to attenuate olanzapine-induced weight gain and improve cognitive flexibility in rats, potentially through mechanisms involving acetate, the major short-chain fatty acid (SCFA) that is produced by B-GOS® fermentation.
30763656	2	2	theme	fatty	630:634	arg1	acetate					599:605	acetate	599:605	acetate	599:605	Ingestion of the prebiotic Bimuno™ galacto-oligosaccharide (B-GOS®) is one such intervention that has been shown to attenuate olanzapine-induced weight gain and improve cognitive flexibility in rats, potentially through mechanisms involving acetate, the major short-chain fatty acid (SCFA) that is produced by B-GOS® fermentation.
30763656	2	2	theme	fatty	630:634	arg1	SCFA					642:645	SCFA	642:645	SCFA	642:645	Ingestion of the prebiotic Bimuno™ galacto-oligosaccharide (B-GOS®) is one such intervention that has been shown to attenuate olanzapine-induced weight gain and improve cognitive flexibility in rats, potentially through mechanisms involving acetate, the major short-chain fatty acid (SCFA) that is produced by B-GOS® fermentation.
30763656	2	2	theme	fatty	630:634	arg1	acid					636:639	the major short-chain fatty acid	608:639	the major short-chain fatty acid (SCFA) that is produced by B-GOS® fermentation	608:686	Ingestion of the prebiotic Bimuno™ galacto-oligosaccharide (B-GOS®) is one such intervention that has been shown to attenuate olanzapine-induced weight gain and improve cognitive flexibility in rats, potentially through mechanisms involving acetate, the major short-chain fatty acid (SCFA) that is produced by B-GOS® fermentation.
30763656	5	3	theme	elevated	1177:1184	arg1	levels					1186:1191	elevated levels	1177:1191	elevated levels of cortical HDAC-1 and HDAC-3 mRNAs	1177:1227	Daily ingestion of B-GOS® for 21 days, reduced HDAC activity and hippocampal HDAC-4, and elevated levels of cortical HDAC-1 and HDAC-3 mRNAs.
30763656	1	4	theme	intestinal	139:148	arg1	microbiome					150:159	The intestinal microbiome	135:159	The intestinal microbiome	135:159	The intestinal microbiome is emerging as a novel therapeutic target owing to the wide range of potential health benefits that could result by manipulating the microbiota composition through relatively simple interventions.
30763656	5	5	theme	HDAC-3	1216:1221	arg1	mRNAs					1223:1227	cortical HDAC-1 and HDAC-3 mRNAs	1196:1227	cortical HDAC-1 and HDAC-3 mRNAs	1196:1227	Daily ingestion of B-GOS® for 21 days, reduced HDAC activity and hippocampal HDAC-4, and elevated levels of cortical HDAC-1 and HDAC-3 mRNAs.
30763656	2	6	theme	olanzapine-induced	484:501	arg1	gain					510:513	olanzapine-induced weight gain	484:513	olanzapine-induced weight gain	484:513	Ingestion of the prebiotic Bimuno™ galacto-oligosaccharide (B-GOS®) is one such intervention that has been shown to attenuate olanzapine-induced weight gain and improve cognitive flexibility in rats, potentially through mechanisms involving acetate, the major short-chain fatty acid (SCFA) that is produced by B-GOS® fermentation.
30763656	0	7	from	reduction	10:18	arg1	rats					104:107	rats	104:107	rats	104:107	Prebiotic reduction of brain histone deacetylase (HDAC) activity and olanzapine-mediated weight gain in rats, are acetate independent.
30763656	2	8	theme	short-chain	618:628	arg1	acetate					599:605	acetate	599:605	acetate	599:605	Ingestion of the prebiotic Bimuno™ galacto-oligosaccharide (B-GOS®) is one such intervention that has been shown to attenuate olanzapine-induced weight gain and improve cognitive flexibility in rats, potentially through mechanisms involving acetate, the major short-chain fatty acid (SCFA) that is produced by B-GOS® fermentation.
30763656	2	8	theme	short-chain	618:628	arg1	SCFA					642:645	SCFA	642:645	SCFA	642:645	Ingestion of the prebiotic Bimuno™ galacto-oligosaccharide (B-GOS®) is one such intervention that has been shown to attenuate olanzapine-induced weight gain and improve cognitive flexibility in rats, potentially through mechanisms involving acetate, the major short-chain fatty acid (SCFA) that is produced by B-GOS® fermentation.
30763656	2	8	theme	short-chain	618:628	arg1	acid					636:639	the major short-chain fatty acid	608:639	the major short-chain fatty acid (SCFA) that is produced by B-GOS® fermentation	608:686	Ingestion of the prebiotic Bimuno™ galacto-oligosaccharide (B-GOS®) is one such intervention that has been shown to attenuate olanzapine-induced weight gain and improve cognitive flexibility in rats, potentially through mechanisms involving acetate, the major short-chain fatty acid (SCFA) that is produced by B-GOS® fermentation.
30763656	8	9	dep	suggests	1505:1512	arg1	replicated					1554:1563	replicated	1554:1563	cannot be replicated by acetate ingestion	1544:1584	Together these data suggests the effects of B-GOS® in rats cannot be replicated by acetate ingestion, and that mechanisms beyond the production of this SCFA are likely to underlie the psychotropic and metabolic actions of this prebiotic.
30763656	8	9	dep	suggests	1505:1512	arg1	likely					1646:1651	likely	1646:1651	likely	1646:1651	Together these data suggests the effects of B-GOS® in rats cannot be replicated by acetate ingestion, and that mechanisms beyond the production of this SCFA are likely to underlie the psychotropic and metabolic actions of this prebiotic.
30763656	1	10	theme	potential	230:238	arg1	benefits					247:254	potential health benefits	230:254	potential health benefits that could result by manipulating the microbiota composition through relatively simple interventions	230:355	The intestinal microbiome is emerging as a novel therapeutic target owing to the wide range of potential health benefits that could result by manipulating the microbiota composition through relatively simple interventions.
30763656	2	11	theme	major	612:616	arg1	acetate					599:605	acetate	599:605	acetate	599:605	Ingestion of the prebiotic Bimuno™ galacto-oligosaccharide (B-GOS®) is one such intervention that has been shown to attenuate olanzapine-induced weight gain and improve cognitive flexibility in rats, potentially through mechanisms involving acetate, the major short-chain fatty acid (SCFA) that is produced by B-GOS® fermentation.
30763656	2	11	theme	major	612:616	arg1	SCFA					642:645	SCFA	642:645	SCFA	642:645	Ingestion of the prebiotic Bimuno™ galacto-oligosaccharide (B-GOS®) is one such intervention that has been shown to attenuate olanzapine-induced weight gain and improve cognitive flexibility in rats, potentially through mechanisms involving acetate, the major short-chain fatty acid (SCFA) that is produced by B-GOS® fermentation.
30763656	2	11	theme	major	612:616	arg1	acid					636:639	the major short-chain fatty acid	608:639	the major short-chain fatty acid (SCFA) that is produced by B-GOS® fermentation	608:686	Ingestion of the prebiotic Bimuno™ galacto-oligosaccharide (B-GOS®) is one such intervention that has been shown to attenuate olanzapine-induced weight gain and improve cognitive flexibility in rats, potentially through mechanisms involving acetate, the major short-chain fatty acid (SCFA) that is produced by B-GOS® fermentation.
30763656	7	12	theme	acetate	1469:1475	arg1	intake					1477:1482	sodium acetate intake	1462:1482	sodium acetate intake	1462:1482	Olanzapine-induced weight gain and fourteen genera of intestinal bacteria, were not influenced by sodium acetate intake.
30763656	3	13	theme	hippocampal	879:889	arg1	expression					891:900	cortical and hippocampal expression	866:900	cortical and hippocampal expression of HDAC1-4	866:911	The present study investigated the individual influences of B-GOS® and sodium acetate intake on brain histone acetyltransferase (HAT) and histone deacetylase (HDAC) activities, cortical and hippocampal expression of HDAC1-4 and N-methyl-d-aspartate receptor subunits in saline or olanzapine injected female rats.
30763656	1	14	theme	health	240:245	arg1	benefits					247:254	potential health benefits	230:254	potential health benefits that could result by manipulating the microbiota composition through relatively simple interventions	230:355	The intestinal microbiome is emerging as a novel therapeutic target owing to the wide range of potential health benefits that could result by manipulating the microbiota composition through relatively simple interventions.
30763656	0	15	theme	weight	89:94	arg1	gain					96:99	olanzapine-mediated weight gain	69:99	olanzapine-mediated weight gain in rats	69:107	Prebiotic reduction of brain histone deacetylase (HDAC) activity and olanzapine-mediated weight gain in rats, are acetate independent.
30763656	3	16	theme	present	693:699	arg1	study					701:705	The present study	689:705	The present study	689:705	The present study investigated the individual influences of B-GOS® and sodium acetate intake on brain histone acetyltransferase (HAT) and histone deacetylase (HDAC) activities, cortical and hippocampal expression of HDAC1-4 and N-methyl-d-aspartate receptor subunits in saline or olanzapine injected female rats.
30763656	6	17	theme	HDAC	1298:1301	arg1	activity					1304:1311	HAT, but not HDAC, activity	1285:1311	HAT, but not HDAC, activity	1285:1311	Sodium acetate supplementation significantly decreased HAT, but not HDAC, activity and increased hippocampal HDAC-3 and HDAC-4 mRNAs.
30763656	8	18	from	effects	1518:1524	arg1	rats					1539:1542	rats	1539:1542	rats	1539:1542	Together these data suggests the effects of B-GOS® in rats cannot be replicated by acetate ingestion, and that mechanisms beyond the production of this SCFA are likely to underlie the psychotropic and metabolic actions of this prebiotic.
30763656	3	19	theme	N-methyl-d-aspartate	917:936	arg1	subunits					947:954	N-methyl-d-aspartate receptor subunits	917:954	N-methyl-d-aspartate receptor subunits	917:954	The present study investigated the individual influences of B-GOS® and sodium acetate intake on brain histone acetyltransferase (HAT) and histone deacetylase (HDAC) activities, cortical and hippocampal expression of HDAC1-4 and N-methyl-d-aspartate receptor subunits in saline or olanzapine injected female rats.
30763656	1	20	theme	benefits	247:254	arg1	range					221:225	the wide range	212:225	the wide range of potential health benefits that could result by manipulating the microbiota composition through relatively simple interventions	212:355	The intestinal microbiome is emerging as a novel therapeutic target owing to the wide range of potential health benefits that could result by manipulating the microbiota composition through relatively simple interventions.
30763656	2	21	theme	cognitive	527:535	arg1	flexibility					537:547	cognitive flexibility	527:547	cognitive flexibility in rats	527:555	Ingestion of the prebiotic Bimuno™ galacto-oligosaccharide (B-GOS®) is one such intervention that has been shown to attenuate olanzapine-induced weight gain and improve cognitive flexibility in rats, potentially through mechanisms involving acetate, the major short-chain fatty acid (SCFA) that is produced by B-GOS® fermentation.
30763656	3	22	theme	B-GOS®	749:754	arg1	influences					735:744	the individual influences	720:744	the individual influences of B-GOS® and sodium acetate intake on brain histone acetyltransferase (HAT) and histone deacetylase (HDAC) activities, cortical and hippocampal expression of HDAC1-4 and N-methyl-d-aspartate receptor subunits	720:954	The present study investigated the individual influences of B-GOS® and sodium acetate intake on brain histone acetyltransferase (HAT) and histone deacetylase (HDAC) activities, cortical and hippocampal expression of HDAC1-4 and N-methyl-d-aspartate receptor subunits in saline or olanzapine injected female rats.
30763656	3	23	theme	receptor	938:945	arg1	subunits					947:954	N-methyl-d-aspartate receptor subunits	917:954	N-methyl-d-aspartate receptor subunits	917:954	The present study investigated the individual influences of B-GOS® and sodium acetate intake on brain histone acetyltransferase (HAT) and histone deacetylase (HDAC) activities, cortical and hippocampal expression of HDAC1-4 and N-methyl-d-aspartate receptor subunits in saline or olanzapine injected female rats.
30763656	3	24	theme	deacetylase	835:845	arg1	activities					854:863	brain histone acetyltransferase (HAT) and histone deacetylase (HDAC) activities	785:863	brain histone acetyltransferase (HAT) and histone deacetylase (HDAC) activities	785:863	The present study investigated the individual influences of B-GOS® and sodium acetate intake on brain histone acetyltransferase (HAT) and histone deacetylase (HDAC) activities, cortical and hippocampal expression of HDAC1-4 and N-methyl-d-aspartate receptor subunits in saline or olanzapine injected female rats.
30763656	3	25	theme	individual	724:733	arg1	influences					735:744	the individual influences	720:744	the individual influences of B-GOS® and sodium acetate intake on brain histone acetyltransferase (HAT) and histone deacetylase (HDAC) activities, cortical and hippocampal expression of HDAC1-4 and N-methyl-d-aspartate receptor subunits	720:954	The present study investigated the individual influences of B-GOS® and sodium acetate intake on brain histone acetyltransferase (HAT) and histone deacetylase (HDAC) activities, cortical and hippocampal expression of HDAC1-4 and N-methyl-d-aspartate receptor subunits in saline or olanzapine injected female rats.
30763656	4	26	theme	acetate	1023:1029	arg1	effect					1006:1011	The effect	1002:1011	The effect of sodium acetate on olanzapine-induced weight gain	1002:1063	The effect of sodium acetate on olanzapine-induced weight gain was also investigated.
30763656	0	27	theme	Prebiotic	0:8	arg1	reduction					10:18	Prebiotic reduction	0:18	Prebiotic reduction of brain histone deacetylase (HDAC) activity	0:63	Prebiotic reduction of brain histone deacetylase (HDAC) activity and olanzapine-mediated weight gain in rats, are acetate independent.
30763656	3	28	theme	HDAC1-4	905:911	arg1	expression					891:900	cortical and hippocampal expression	866:900	cortical and hippocampal expression of HDAC1-4	866:911	The present study investigated the individual influences of B-GOS® and sodium acetate intake on brain histone acetyltransferase (HAT) and histone deacetylase (HDAC) activities, cortical and hippocampal expression of HDAC1-4 and N-methyl-d-aspartate receptor subunits in saline or olanzapine injected female rats.
30763656	3	28	theme	HDAC1-4	905:911	arg1	activities					854:863	brain histone acetyltransferase (HAT) and histone deacetylase (HDAC) activities	785:863	brain histone acetyltransferase (HAT) and histone deacetylase (HDAC) activities	785:863	The present study investigated the individual influences of B-GOS® and sodium acetate intake on brain histone acetyltransferase (HAT) and histone deacetylase (HDAC) activities, cortical and hippocampal expression of HDAC1-4 and N-methyl-d-aspartate receptor subunits in saline or olanzapine injected female rats.
30763656	3	28	theme	HDAC1-4	905:911	arg1	subunits					947:954	N-methyl-d-aspartate receptor subunits	917:954	N-methyl-d-aspartate receptor subunits	917:954	The present study investigated the individual influences of B-GOS® and sodium acetate intake on brain histone acetyltransferase (HAT) and histone deacetylase (HDAC) activities, cortical and hippocampal expression of HDAC1-4 and N-methyl-d-aspartate receptor subunits in saline or olanzapine injected female rats.
30763656	2	29	theme	galacto-oligosaccharide	393:415	arg1	Ingestion					358:366	Ingestion	358:366	Ingestion of the prebiotic Bimuno™ galacto-oligosaccharide (B-GOS®)	358:424	Ingestion of the prebiotic Bimuno™ galacto-oligosaccharide (B-GOS®) is one such intervention that has been shown to attenuate olanzapine-induced weight gain and improve cognitive flexibility in rats, potentially through mechanisms involving acetate, the major short-chain fatty acid (SCFA) that is produced by B-GOS® fermentation.
30763656	5	30	theme	HDAC	1135:1138	arg1	activity					1140:1147	HDAC activity	1135:1147	HDAC activity	1135:1147	Daily ingestion of B-GOS® for 21 days, reduced HDAC activity and hippocampal HDAC-4, and elevated levels of cortical HDAC-1 and HDAC-3 mRNAs.
30763656	2	31	theme	Bimuno™	385:391	arg1	galacto-oligosaccharide					393:415	the prebiotic Bimuno™ galacto-oligosaccharide	371:415	the prebiotic Bimuno™ galacto-oligosaccharide (B-GOS®)	371:424	Ingestion of the prebiotic Bimuno™ galacto-oligosaccharide (B-GOS®) is one such intervention that has been shown to attenuate olanzapine-induced weight gain and improve cognitive flexibility in rats, potentially through mechanisms involving acetate, the major short-chain fatty acid (SCFA) that is produced by B-GOS® fermentation.
30763656	2	31	theme	Bimuno™	385:391	arg1	B-GOS®					418:423	B-GOS®	418:423	B-GOS®	418:423	Ingestion of the prebiotic Bimuno™ galacto-oligosaccharide (B-GOS®) is one such intervention that has been shown to attenuate olanzapine-induced weight gain and improve cognitive flexibility in rats, potentially through mechanisms involving acetate, the major short-chain fatty acid (SCFA) that is produced by B-GOS® fermentation.
30763656	1	32	theme	simple	336:341	arg1	interventions					343:355	relatively simple interventions	325:355	relatively simple interventions	325:355	The intestinal microbiome is emerging as a novel therapeutic target owing to the wide range of potential health benefits that could result by manipulating the microbiota composition through relatively simple interventions.
30763656	3	33	theme	acetate	767:773	arg1	intake					775:780	sodium acetate intake	760:780	sodium acetate intake	760:780	The present study investigated the individual influences of B-GOS® and sodium acetate intake on brain histone acetyltransferase (HAT) and histone deacetylase (HDAC) activities, cortical and hippocampal expression of HDAC1-4 and N-methyl-d-aspartate receptor subunits in saline or olanzapine injected female rats.
30763656	6	34	theme	HAT	1285:1287	arg1	activity					1304:1311	HAT, but not HDAC, activity	1285:1311	HAT, but not HDAC, activity	1285:1311	Sodium acetate supplementation significantly decreased HAT, but not HDAC, activity and increased hippocampal HDAC-3 and HDAC-4 mRNAs.
30763656	4	35	theme	weight	1053:1058	arg1	gain					1060:1063	olanzapine-induced weight gain	1034:1063	olanzapine-induced weight gain	1034:1063	The effect of sodium acetate on olanzapine-induced weight gain was also investigated.
30763656	2	36	theme	prebiotic	375:383	arg1	galacto-oligosaccharide					393:415	the prebiotic Bimuno™ galacto-oligosaccharide	371:415	the prebiotic Bimuno™ galacto-oligosaccharide (B-GOS®)	371:424	Ingestion of the prebiotic Bimuno™ galacto-oligosaccharide (B-GOS®) is one such intervention that has been shown to attenuate olanzapine-induced weight gain and improve cognitive flexibility in rats, potentially through mechanisms involving acetate, the major short-chain fatty acid (SCFA) that is produced by B-GOS® fermentation.
30763656	2	36	theme	prebiotic	375:383	arg1	B-GOS®					418:423	B-GOS®	418:423	B-GOS®	418:423	Ingestion of the prebiotic Bimuno™ galacto-oligosaccharide (B-GOS®) is one such intervention that has been shown to attenuate olanzapine-induced weight gain and improve cognitive flexibility in rats, potentially through mechanisms involving acetate, the major short-chain fatty acid (SCFA) that is produced by B-GOS® fermentation.
30763656	0	37	theme	brain	23:27	arg1	deacetylase					37:47	brain histone deacetylase	23:47	brain histone deacetylase (HDAC) activity	23:63	Prebiotic reduction of brain histone deacetylase (HDAC) activity and olanzapine-mediated weight gain in rats, are acetate independent.
30763656	0	37	theme	brain	23:27	arg1	HDAC					50:53	HDAC	50:53	HDAC	50:53	Prebiotic reduction of brain histone deacetylase (HDAC) activity and olanzapine-mediated weight gain in rats, are acetate independent.
30763656	3	38	theme	intake	775:780	arg1	influences					735:744	the individual influences	720:744	the individual influences of B-GOS® and sodium acetate intake on brain histone acetyltransferase (HAT) and histone deacetylase (HDAC) activities, cortical and hippocampal expression of HDAC1-4 and N-methyl-d-aspartate receptor subunits	720:954	The present study investigated the individual influences of B-GOS® and sodium acetate intake on brain histone acetyltransferase (HAT) and histone deacetylase (HDAC) activities, cortical and hippocampal expression of HDAC1-4 and N-methyl-d-aspartate receptor subunits in saline or olanzapine injected female rats.
30763656	2	39	theme	B-GOS®	668:673	arg1	fermentation					675:686	B-GOS® fermentation	668:686	B-GOS® fermentation	668:686	Ingestion of the prebiotic Bimuno™ galacto-oligosaccharide (B-GOS®) is one such intervention that has been shown to attenuate olanzapine-induced weight gain and improve cognitive flexibility in rats, potentially through mechanisms involving acetate, the major short-chain fatty acid (SCFA) that is produced by B-GOS® fermentation.
30763656	1	40	theme	novel	178:182	arg1	target					196:201	a novel therapeutic target	176:201	a novel therapeutic target owing to the wide range of potential health benefits that could result by manipulating the microbiota composition through relatively simple interventions	176:355	The intestinal microbiome is emerging as a novel therapeutic target owing to the wide range of potential health benefits that could result by manipulating the microbiota composition through relatively simple interventions.
30763656	5	41	theme	HDAC-1	1205:1210	arg1	mRNAs					1223:1227	cortical HDAC-1 and HDAC-3 mRNAs	1196:1227	cortical HDAC-1 and HDAC-3 mRNAs	1196:1227	Daily ingestion of B-GOS® for 21 days, reduced HDAC activity and hippocampal HDAC-4, and elevated levels of cortical HDAC-1 and HDAC-3 mRNAs.
30763656	1	42	theme	therapeutic	184:194	arg1	target					196:201	a novel therapeutic target	176:201	a novel therapeutic target owing to the wide range of potential health benefits that could result by manipulating the microbiota composition through relatively simple interventions	176:355	The intestinal microbiome is emerging as a novel therapeutic target owing to the wide range of potential health benefits that could result by manipulating the microbiota composition through relatively simple interventions.
30763656	0	43	theme	deacetylase	37:47	arg1	activity					56:63	brain histone deacetylase (HDAC) activity	23:63	brain histone deacetylase (HDAC) activity	23:63	Prebiotic reduction of brain histone deacetylase (HDAC) activity and olanzapine-mediated weight gain in rats, are acetate independent.
30763656	3	44	theme	sodium	760:765	arg1	intake					775:780	sodium acetate intake	760:780	sodium acetate intake	760:780	The present study investigated the individual influences of B-GOS® and sodium acetate intake on brain histone acetyltransferase (HAT) and histone deacetylase (HDAC) activities, cortical and hippocampal expression of HDAC1-4 and N-methyl-d-aspartate receptor subunits in saline or olanzapine injected female rats.
30763656	5	45	theme	B-GOS®	1107:1112	arg1	ingestion					1094:1102	Daily ingestion	1088:1102	Daily ingestion of B-GOS®	1088:1112	Daily ingestion of B-GOS® for 21 days, reduced HDAC activity and hippocampal HDAC-4, and elevated levels of cortical HDAC-1 and HDAC-3 mRNAs.
30763656	7	46	theme	intestinal	1418:1427	arg1	bacteria					1429:1436	intestinal bacteria	1418:1436	intestinal bacteria	1418:1436	Olanzapine-induced weight gain and fourteen genera of intestinal bacteria, were not influenced by sodium acetate intake.
30763656	8	47	theme	metabolic	1686:1694	arg1	actions					1696:1702	the psychotropic and metabolic actions	1665:1702	the psychotropic and metabolic actions of this prebiotic	1665:1720	Together these data suggests the effects of B-GOS® in rats cannot be replicated by acetate ingestion, and that mechanisms beyond the production of this SCFA are likely to underlie the psychotropic and metabolic actions of this prebiotic.
30763656	5	48	theme	mRNAs	1223:1227	arg1	levels					1186:1191	elevated levels	1177:1191	elevated levels of cortical HDAC-1 and HDAC-3 mRNAs	1177:1227	Daily ingestion of B-GOS® for 21 days, reduced HDAC activity and hippocampal HDAC-4, and elevated levels of cortical HDAC-1 and HDAC-3 mRNAs.
30763656	5	48	theme	mRNAs	1223:1227	arg1	activity					1140:1147	HDAC activity	1135:1147	HDAC activity	1135:1147	Daily ingestion of B-GOS® for 21 days, reduced HDAC activity and hippocampal HDAC-4, and elevated levels of cortical HDAC-1 and HDAC-3 mRNAs.
30763656	5	48	theme	mRNAs	1223:1227	arg1	HDAC-4					1165:1170	hippocampal HDAC-4	1153:1170	hippocampal HDAC-4	1153:1170	Daily ingestion of B-GOS® for 21 days, reduced HDAC activity and hippocampal HDAC-4, and elevated levels of cortical HDAC-1 and HDAC-3 mRNAs.
30763656	0	49	theme	histone	29:35	arg1	deacetylase					37:47	brain histone deacetylase	23:47	brain histone deacetylase (HDAC) activity	23:63	Prebiotic reduction of brain histone deacetylase (HDAC) activity and olanzapine-mediated weight gain in rats, are acetate independent.
30763656	0	49	theme	histone	29:35	arg1	HDAC					50:53	HDAC	50:53	HDAC	50:53	Prebiotic reduction of brain histone deacetylase (HDAC) activity and olanzapine-mediated weight gain in rats, are acetate independent.
30763656	3	50	theme	histone	791:797	arg1	HAT					818:820	HAT	818:820	HAT	818:820	The present study investigated the individual influences of B-GOS® and sodium acetate intake on brain histone acetyltransferase (HAT) and histone deacetylase (HDAC) activities, cortical and hippocampal expression of HDAC1-4 and N-methyl-d-aspartate receptor subunits in saline or olanzapine injected female rats.
30763656	3	50	theme	histone	791:797	arg1	acetyltransferase					799:815	brain histone acetyltransferase	785:815	brain histone acetyltransferase (HAT)	785:821	The present study investigated the individual influences of B-GOS® and sodium acetate intake on brain histone acetyltransferase (HAT) and histone deacetylase (HDAC) activities, cortical and hippocampal expression of HDAC1-4 and N-methyl-d-aspartate receptor subunits in saline or olanzapine injected female rats.
30763656	4	51	theme	sodium	1016:1021	arg1	acetate					1023:1029	sodium acetate	1016:1029	sodium acetate	1016:1029	The effect of sodium acetate on olanzapine-induced weight gain was also investigated.
30763656	8	52	theme	psychotropic	1669:1680	arg1	actions					1696:1702	the psychotropic and metabolic actions	1665:1702	the psychotropic and metabolic actions of this prebiotic	1665:1720	Together these data suggests the effects of B-GOS® in rats cannot be replicated by acetate ingestion, and that mechanisms beyond the production of this SCFA are likely to underlie the psychotropic and metabolic actions of this prebiotic.
30763656	7	53	theme	bacteria	1429:1436	arg1	gain					1390:1393	Olanzapine-induced weight gain	1364:1393	Olanzapine-induced weight gain	1364:1393	Olanzapine-induced weight gain and fourteen genera of intestinal bacteria, were not influenced by sodium acetate intake.
30763656	7	53	theme	bacteria	1429:1436	arg1	genera					1408:1413	fourteen genera	1399:1413	fourteen genera of intestinal bacteria	1399:1436	Olanzapine-induced weight gain and fourteen genera of intestinal bacteria, were not influenced by sodium acetate intake.
30763656	3	54	theme	female	989:994	arg1	rats					996:999	injected female rats	980:999	injected female rats	980:999	The present study investigated the individual influences of B-GOS® and sodium acetate intake on brain histone acetyltransferase (HAT) and histone deacetylase (HDAC) activities, cortical and hippocampal expression of HDAC1-4 and N-methyl-d-aspartate receptor subunits in saline or olanzapine injected female rats.
30763656	4	55	theme	olanzapine-induced	1034:1051	arg1	gain					1060:1063	olanzapine-induced weight gain	1034:1063	olanzapine-induced weight gain	1034:1063	The effect of sodium acetate on olanzapine-induced weight gain was also investigated.
30763656	3	56	theme	acetyltransferase	799:815	arg1	activities					854:863	brain histone acetyltransferase (HAT) and histone deacetylase (HDAC) activities	785:863	brain histone acetyltransferase (HAT) and histone deacetylase (HDAC) activities	785:863	The present study investigated the individual influences of B-GOS® and sodium acetate intake on brain histone acetyltransferase (HAT) and histone deacetylase (HDAC) activities, cortical and hippocampal expression of HDAC1-4 and N-methyl-d-aspartate receptor subunits in saline or olanzapine injected female rats.
30763656	5	57	theme	cortical	1196:1203	arg1	mRNAs					1223:1227	cortical HDAC-1 and HDAC-3 mRNAs	1196:1227	cortical HDAC-1 and HDAC-3 mRNAs	1196:1227	Daily ingestion of B-GOS® for 21 days, reduced HDAC activity and hippocampal HDAC-4, and elevated levels of cortical HDAC-1 and HDAC-3 mRNAs.
30763656	8	58	theme	B-GOS®	1529:1534	arg1	effects					1518:1524	the effects	1514:1524	the effects of B-GOS® in rats	1514:1542	Together these data suggests the effects of B-GOS® in rats cannot be replicated by acetate ingestion, and that mechanisms beyond the production of this SCFA are likely to underlie the psychotropic and metabolic actions of this prebiotic.
30763656	8	59	theme	acetate	1568:1574	arg1	ingestion					1576:1584	acetate ingestion	1568:1584	acetate ingestion	1568:1584	Together these data suggests the effects of B-GOS® in rats cannot be replicated by acetate ingestion, and that mechanisms beyond the production of this SCFA are likely to underlie the psychotropic and metabolic actions of this prebiotic.
30763656	5	60	theme	hippocampal	1153:1163	arg1	HDAC-4					1165:1170	hippocampal HDAC-4	1153:1170	hippocampal HDAC-4	1153:1170	Daily ingestion of B-GOS® for 21 days, reduced HDAC activity and hippocampal HDAC-4, and elevated levels of cortical HDAC-1 and HDAC-3 mRNAs.
30763656	6	61	theme	HDAC-4	1350:1355	arg1	mRNAs					1357:1361	HDAC-4 mRNAs	1350:1361	HDAC-4 mRNAs	1350:1361	Sodium acetate supplementation significantly decreased HAT, but not HDAC, activity and increased hippocampal HDAC-3 and HDAC-4 mRNAs.
30763656	1	62	theme	microbiota	294:303	arg1	composition					305:315	the microbiota composition	290:315	the microbiota composition	290:315	The intestinal microbiome is emerging as a novel therapeutic target owing to the wide range of potential health benefits that could result by manipulating the microbiota composition through relatively simple interventions.
30763656	6	63	theme	acetate	1237:1243	arg1	supplementation					1245:1259	Sodium acetate supplementation	1230:1259	Sodium acetate supplementation	1230:1259	Sodium acetate supplementation significantly decreased HAT, but not HDAC, activity and increased hippocampal HDAC-3 and HDAC-4 mRNAs.
30763656	3	64	theme	brain	785:789	arg1	HAT					818:820	HAT	818:820	HAT	818:820	The present study investigated the individual influences of B-GOS® and sodium acetate intake on brain histone acetyltransferase (HAT) and histone deacetylase (HDAC) activities, cortical and hippocampal expression of HDAC1-4 and N-methyl-d-aspartate receptor subunits in saline or olanzapine injected female rats.
30763656	3	64	theme	brain	785:789	arg1	acetyltransferase					799:815	brain histone acetyltransferase	785:815	brain histone acetyltransferase (HAT)	785:821	The present study investigated the individual influences of B-GOS® and sodium acetate intake on brain histone acetyltransferase (HAT) and histone deacetylase (HDAC) activities, cortical and hippocampal expression of HDAC1-4 and N-methyl-d-aspartate receptor subunits in saline or olanzapine injected female rats.
30763656	6	65	theme	hippocampal	1327:1337	arg1	HDAC-3					1339:1344	hippocampal HDAC-3	1327:1344	hippocampal HDAC-3	1327:1344	Sodium acetate supplementation significantly decreased HAT, but not HDAC, activity and increased hippocampal HDAC-3 and HDAC-4 mRNAs.
30763656	3	66	from	influences	735:744	arg1	expression					891:900	cortical and hippocampal expression	866:900	cortical and hippocampal expression of HDAC1-4	866:911	The present study investigated the individual influences of B-GOS® and sodium acetate intake on brain histone acetyltransferase (HAT) and histone deacetylase (HDAC) activities, cortical and hippocampal expression of HDAC1-4 and N-methyl-d-aspartate receptor subunits in saline or olanzapine injected female rats.
30763656	3	66	from	influences	735:744	arg1	activities					854:863	brain histone acetyltransferase (HAT) and histone deacetylase (HDAC) activities	785:863	brain histone acetyltransferase (HAT) and histone deacetylase (HDAC) activities	785:863	The present study investigated the individual influences of B-GOS® and sodium acetate intake on brain histone acetyltransferase (HAT) and histone deacetylase (HDAC) activities, cortical and hippocampal expression of HDAC1-4 and N-methyl-d-aspartate receptor subunits in saline or olanzapine injected female rats.
30763656	3	66	from	influences	735:744	arg1	subunits					947:954	N-methyl-d-aspartate receptor subunits	917:954	N-methyl-d-aspartate receptor subunits	917:954	The present study investigated the individual influences of B-GOS® and sodium acetate intake on brain histone acetyltransferase (HAT) and histone deacetylase (HDAC) activities, cortical and hippocampal expression of HDAC1-4 and N-methyl-d-aspartate receptor subunits in saline or olanzapine injected female rats.
30763656	6	67	theme	Sodium	1230:1235	arg1	supplementation					1245:1259	Sodium acetate supplementation	1230:1259	Sodium acetate supplementation	1230:1259	Sodium acetate supplementation significantly decreased HAT, but not HDAC, activity and increased hippocampal HDAC-3 and HDAC-4 mRNAs.
30763656	3	68	theme	injected	980:987	arg1	rats					996:999	injected female rats	980:999	injected female rats	980:999	The present study investigated the individual influences of B-GOS® and sodium acetate intake on brain histone acetyltransferase (HAT) and histone deacetylase (HDAC) activities, cortical and hippocampal expression of HDAC1-4 and N-methyl-d-aspartate receptor subunits in saline or olanzapine injected female rats.
30763656	3	69	theme	cortical	866:873	arg1	expression					891:900	cortical and hippocampal expression	866:900	cortical and hippocampal expression of HDAC1-4	866:911	The present study investigated the individual influences of B-GOS® and sodium acetate intake on brain histone acetyltransferase (HAT) and histone deacetylase (HDAC) activities, cortical and hippocampal expression of HDAC1-4 and N-methyl-d-aspartate receptor subunits in saline or olanzapine injected female rats.
30763656	7	70	theme	sodium	1462:1467	arg1	intake					1477:1482	sodium acetate intake	1462:1482	sodium acetate intake	1462:1482	Olanzapine-induced weight gain and fourteen genera of intestinal bacteria, were not influenced by sodium acetate intake.
30763656	0	71	from	gain	96:99	arg1	rats					104:107	rats	104:107	rats	104:107	Prebiotic reduction of brain histone deacetylase (HDAC) activity and olanzapine-mediated weight gain in rats, are acetate independent.
30763656	5	72	theme	Daily	1088:1092	arg1	ingestion					1094:1102	Daily ingestion	1088:1102	Daily ingestion of B-GOS®	1088:1112	Daily ingestion of B-GOS® for 21 days, reduced HDAC activity and hippocampal HDAC-4, and elevated levels of cortical HDAC-1 and HDAC-3 mRNAs.
30763656	4	73	from	effect	1006:1011	arg1	gain					1060:1063	olanzapine-induced weight gain	1034:1063	olanzapine-induced weight gain	1034:1063	The effect of sodium acetate on olanzapine-induced weight gain was also investigated.
30763656	0	74	theme	activity	56:63	arg1	gain					96:99	olanzapine-mediated weight gain	69:99	olanzapine-mediated weight gain in rats	69:107	Prebiotic reduction of brain histone deacetylase (HDAC) activity and olanzapine-mediated weight gain in rats, are acetate independent.
30763656	0	74	theme	activity	56:63	arg1	reduction					10:18	Prebiotic reduction	0:18	Prebiotic reduction of brain histone deacetylase (HDAC) activity	0:63	Prebiotic reduction of brain histone deacetylase (HDAC) activity and olanzapine-mediated weight gain in rats, are acetate independent.
30763656	2	75	from	flexibility	537:547	arg1	rats					552:555	rats	552:555	rats	552:555	Ingestion of the prebiotic Bimuno™ galacto-oligosaccharide (B-GOS®) is one such intervention that has been shown to attenuate olanzapine-induced weight gain and improve cognitive flexibility in rats, potentially through mechanisms involving acetate, the major short-chain fatty acid (SCFA) that is produced by B-GOS® fermentation.
30763656	7	76	theme	weight	1383:1388	arg1	gain					1390:1393	Olanzapine-induced weight gain	1364:1393	Olanzapine-induced weight gain	1364:1393	Olanzapine-induced weight gain and fourteen genera of intestinal bacteria, were not influenced by sodium acetate intake.
30763656	8	77	theme	prebiotic	1712:1720	arg1	actions					1696:1702	the psychotropic and metabolic actions	1665:1702	the psychotropic and metabolic actions of this prebiotic	1665:1720	Together these data suggests the effects of B-GOS® in rats cannot be replicated by acetate ingestion, and that mechanisms beyond the production of this SCFA are likely to underlie the psychotropic and metabolic actions of this prebiotic.
30763656	7	78	theme	Olanzapine-induced	1364:1381	arg1	gain					1390:1393	Olanzapine-induced weight gain	1364:1393	Olanzapine-induced weight gain	1364:1393	Olanzapine-induced weight gain and fourteen genera of intestinal bacteria, were not influenced by sodium acetate intake.
30763656	1	79	theme	wide	216:219	arg1	range					221:225	the wide range	212:225	the wide range of potential health benefits that could result by manipulating the microbiota composition through relatively simple interventions	212:355	The intestinal microbiome is emerging as a novel therapeutic target owing to the wide range of potential health benefits that could result by manipulating the microbiota composition through relatively simple interventions.
30763656	3	80	theme	histone	827:833	arg1	deacetylase					835:845	histone deacetylase	827:845	histone deacetylase (HDAC)	827:852	The present study investigated the individual influences of B-GOS® and sodium acetate intake on brain histone acetyltransferase (HAT) and histone deacetylase (HDAC) activities, cortical and hippocampal expression of HDAC1-4 and N-methyl-d-aspartate receptor subunits in saline or olanzapine injected female rats.
30763656	3	80	theme	histone	827:833	arg1	HDAC					848:851	HDAC	848:851	HDAC	848:851	The present study investigated the individual influences of B-GOS® and sodium acetate intake on brain histone acetyltransferase (HAT) and histone deacetylase (HDAC) activities, cortical and hippocampal expression of HDAC1-4 and N-methyl-d-aspartate receptor subunits in saline or olanzapine injected female rats.
30763656	3	81	dep	saline	959:964	arg1	rats					996:999	injected female rats	980:999	injected female rats	980:999	The present study investigated the individual influences of B-GOS® and sodium acetate intake on brain histone acetyltransferase (HAT) and histone deacetylase (HDAC) activities, cortical and hippocampal expression of HDAC1-4 and N-methyl-d-aspartate receptor subunits in saline or olanzapine injected female rats.
30763656	8	82	theme	SCFA	1637:1640	arg1	production					1618:1627	the production	1614:1627	the production of this SCFA	1614:1640	Together these data suggests the effects of B-GOS® in rats cannot be replicated by acetate ingestion, and that mechanisms beyond the production of this SCFA are likely to underlie the psychotropic and metabolic actions of this prebiotic.
29485180	7	0	theme	cell	1566:1569	arg1	types					1571:1575	both cartilage-derived cell types	1543:1575	both cartilage-derived cell types	1543:1575	Similar to MSCs and chondrocytes, AuCPCs displayed a marked ability to generate cartilaginous matrix, although, under the applied culture conditions, MSCs outperformed both cartilage-derived cell types in terms of matrix production and mechanical properties.
29485180	9	1	theme	tissue-engineered	1994:2010	arg1	constructs					2012:2021	elastic cartilage tissue-engineered constructs	1976:2021	elastic cartilage tissue-engineered constructs	1976:2021	These results underscored the AuCPCs' tissue-specific differentiation potential, making them an interesting cell source for the next generation of elastic cartilage tissue-engineered constructs.
29485180	8	2	theme	collagen	1712:1719	arg1	X					1726:1726	collagen type X	1712:1726	collagen type X	1712:1726	AuCPCs demonstrated upregulated mRNA expression of elastin, low expression of collagen type X and similar levels of proteoglycan production and mechanical properties as compared to chondrocytes.
29485180	6	3	theme	biochemical	1336:1346	arg1	composition					1348:1358	biochemical composition	1336:1358	biochemical composition	1336:1358	Neocartilage formation was assessed through gene expression profiling, compression testing, biochemical composition and histology.
29485180	3	4	theme	neo-tissues	516:526	arg1	quality					505:511	the quality	501:511	the quality of neo-tissues resulting from these cell types	501:558	However, the quality of neo-tissues resulting from these cell types is inadequate due to inefficient chondrogenic differentiation and endochondral ossification, respectively.
29485180	3	4	theme	neo-tissues	516:526	arg1	inadequate					563:572	inadequate	563:572	inadequate	563:572	However, the quality of neo-tissues resulting from these cell types is inadequate due to inefficient chondrogenic differentiation and endochondral ossification, respectively.
29485180	8	5	theme	mechanical	1778:1787	arg1	properties					1789:1798	mechanical properties	1778:1798	mechanical properties	1778:1798	AuCPCs demonstrated upregulated mRNA expression of elastin, low expression of collagen type X and similar levels of proteoglycan production and mechanical properties as compared to chondrocytes.
29485180	8	6	theme	X	1726:1726	arg1	levels					1740:1745	similar levels	1732:1745	similar levels of proteoglycan production and mechanical properties	1732:1798	AuCPCs demonstrated upregulated mRNA expression of elastin, low expression of collagen type X and similar levels of proteoglycan production and mechanical properties as compared to chondrocytes.
29485180	8	6	theme	X	1726:1726	arg1	expression					1698:1707	low expression	1694:1707	low expression of collagen type X	1694:1726	AuCPCs demonstrated upregulated mRNA expression of elastin, low expression of collagen type X and similar levels of proteoglycan production and mechanical properties as compared to chondrocytes.
29485180	8	6	theme	X	1726:1726	arg1	expression					1671:1680	upregulated mRNA expression	1654:1680	upregulated mRNA expression of elastin	1654:1691	AuCPCs demonstrated upregulated mRNA expression of elastin, low expression of collagen type X and similar levels of proteoglycan production and mechanical properties as compared to chondrocytes.
29485180	4	7	theme	stem/progenitor	696:710	arg1	cells					712:716	stem/progenitor cells	696:716	stem/progenitor cells	696:716	Recently, a subpopulation of stem/progenitor cells has been identified within the auricular cartilage tissue, with similarities to MSCs in terms of proliferative capacity and cell surface biomarkers, but their potential for tissue engineering has not yet been explored.
29485180	3	8	theme	cell	549:552	arg1	types					554:558	these cell types	543:558	these cell types	543:558	However, the quality of neo-tissues resulting from these cell types is inadequate due to inefficient chondrogenic differentiation and endochondral ossification, respectively.
29485180	6	9	theme	expression	1293:1302	arg1	profiling					1304:1312	gene expression profiling	1288:1312	gene expression profiling	1288:1312	Neocartilage formation was assessed through gene expression profiling, compression testing, biochemical composition and histology.
29485180	3	10	theme	inefficient	581:591	arg1	differentiation					606:620	inefficient chondrogenic differentiation	581:620	inefficient chondrogenic differentiation	581:620	However, the quality of neo-tissues resulting from these cell types is inadequate due to inefficient chondrogenic differentiation and endochondral ossification, respectively.
29485180	7	11	theme	cartilaginous	1455:1467	arg1	matrix					1469:1474	cartilaginous matrix	1455:1474	cartilaginous matrix	1455:1474	Similar to MSCs and chondrocytes, AuCPCs displayed a marked ability to generate cartilaginous matrix, although, under the applied culture conditions, MSCs outperformed both cartilage-derived cell types in terms of matrix production and mechanical properties.
29485180	1	12	theme	number	214:219	arg1	acquisition					191:201	the acquisition	187:201	the acquisition of a large number of cells maintaining an elastic cartilage phenotype, which is the key in producing a tissue capable of withstanding forces subjected to the auricle	187:367	Paramount for the generation of auricular structures of clinically-relevant size is the acquisition of a large number of cells maintaining an elastic cartilage phenotype, which is the key in producing a tissue capable of withstanding forces subjected to the auricle.
29485180	6	13	theme	gene	1288:1291	arg1	profiling					1304:1312	gene expression profiling	1288:1312	gene expression profiling	1288:1312	Neocartilage formation was assessed through gene expression profiling, compression testing, biochemical composition and histology.
29485180	3	14	theme	endochondral	626:637	arg1	ossification					639:650	endochondral ossification	626:650	endochondral ossification	626:650	However, the quality of neo-tissues resulting from these cell types is inadequate due to inefficient chondrogenic differentiation and endochondral ossification, respectively.
29485180	1	15	theme	cells	224:228	arg1	number					214:219	a large number	206:219	a large number of cells maintaining an elastic cartilage phenotype, which is the key in producing a tissue capable of withstanding forces subjected to the auricle	206:367	Paramount for the generation of auricular structures of clinically-relevant size is the acquisition of a large number of cells maintaining an elastic cartilage phenotype, which is the key in producing a tissue capable of withstanding forces subjected to the auricle.
29485180	7	16	theme	marked	1428:1433	arg1	ability					1435:1441	a marked ability	1426:1441	a marked ability to generate cartilaginous matrix, although, under the applied culture conditions, MSCs outperformed both cartilage-derived cell types in terms of matrix production and mechanical properties	1426:1631	Similar to MSCs and chondrocytes, AuCPCs displayed a marked ability to generate cartilaginous matrix, although, under the applied culture conditions, MSCs outperformed both cartilage-derived cell types in terms of matrix production and mechanical properties.
29485180	4	17	theme	cartilage	759:767	arg1	tissue					769:774	the auricular cartilage tissue	745:774	the auricular cartilage tissue	745:774	Recently, a subpopulation of stem/progenitor cells has been identified within the auricular cartilage tissue, with similarities to MSCs in terms of proliferative capacity and cell surface biomarkers, but their potential for tissue engineering has not yet been explored.
29485180	5	18	theme	auricular	1082:1090	arg1	chondrocytes					1092:1103	auricular chondrocytes	1082:1103	auricular chondrocytes	1082:1103	This study compared the in vitro cartilage-forming ability of equine auricular cartilage progenitor cells (AuCPCs), bone marrow-derived MSCs and auricular chondrocytes in gelatin methacryloyl (gelMA)-based hydrogels over a period of 56 d, by assessing their ability to undergo chondrogenic differentiation.
29485180	1	19	theme	structures	145:154	arg1	generation					121:130	the generation	117:130	the generation of auricular structures of clinically-relevant size	117:182	Paramount for the generation of auricular structures of clinically-relevant size is the acquisition of a large number of cells maintaining an elastic cartilage phenotype, which is the key in producing a tissue capable of withstanding forces subjected to the auricle.
29485180	5	20	theme	in	961:962	arg1	ability					988:994	the in vitro cartilage-forming ability	957:994	the in vitro cartilage-forming ability of equine auricular cartilage progenitor cells (AuCPCs), bone marrow-derived MSCs and auricular chondrocytes in gelatin methacryloyl (gelMA)-based hydrogels	957:1151	This study compared the in vitro cartilage-forming ability of equine auricular cartilage progenitor cells (AuCPCs), bone marrow-derived MSCs and auricular chondrocytes in gelatin methacryloyl (gelMA)-based hydrogels over a period of 56 d, by assessing their ability to undergo chondrogenic differentiation.
29485180	1	21	theme	clinically-relevant	159:177	arg1	size					179:182	clinically-relevant size	159:182	clinically-relevant size	159:182	Paramount for the generation of auricular structures of clinically-relevant size is the acquisition of a large number of cells maintaining an elastic cartilage phenotype, which is the key in producing a tissue capable of withstanding forces subjected to the auricle.
29485180	0	22	theme	hydrogel	85:92	arg1	culture					94:100	3D hydrogel culture	82:100	3D hydrogel culture	82:100	Progenitor cells in auricular cartilage demonstrate cartilage-forming capacity in 3D hydrogel culture.
29485180	9	23	theme	tissue-specific	1867:1881	arg1	potential					1899:1907	the AuCPCs' tissue-specific differentiation potential	1855:1907	the AuCPCs' tissue-specific differentiation potential	1855:1907	These results underscored the AuCPCs' tissue-specific differentiation potential, making them an interesting cell source for the next generation of elastic cartilage tissue-engineered constructs.
29485180	5	24	dep	in	961:962	arg1	vitro					964:968	vitro	964:968	vitro	964:968	This study compared the in vitro cartilage-forming ability of equine auricular cartilage progenitor cells (AuCPCs), bone marrow-derived MSCs and auricular chondrocytes in gelatin methacryloyl (gelMA)-based hydrogels over a period of 56 d, by assessing their ability to undergo chondrogenic differentiation.
29485180	5	25	theme	-based	1136:1141	arg1	hydrogels					1143:1151	gelatin methacryloyl (gelMA)-based hydrogels	1108:1151	gelatin methacryloyl (gelMA)-based hydrogels	1108:1151	This study compared the in vitro cartilage-forming ability of equine auricular cartilage progenitor cells (AuCPCs), bone marrow-derived MSCs and auricular chondrocytes in gelatin methacryloyl (gelMA)-based hydrogels over a period of 56 d, by assessing their ability to undergo chondrogenic differentiation.
29485180	5	26	theme	cartilage	1016:1024	arg1	AuCPCs					1044:1049	AuCPCs	1044:1049	AuCPCs	1044:1049	This study compared the in vitro cartilage-forming ability of equine auricular cartilage progenitor cells (AuCPCs), bone marrow-derived MSCs and auricular chondrocytes in gelatin methacryloyl (gelMA)-based hydrogels over a period of 56 d, by assessing their ability to undergo chondrogenic differentiation.
29485180	5	26	theme	cartilage	1016:1024	arg1	cells					1037:1041	equine auricular cartilage progenitor cells	999:1041	equine auricular cartilage progenitor cells (AuCPCs)	999:1050	This study compared the in vitro cartilage-forming ability of equine auricular cartilage progenitor cells (AuCPCs), bone marrow-derived MSCs and auricular chondrocytes in gelatin methacryloyl (gelMA)-based hydrogels over a period of 56 d, by assessing their ability to undergo chondrogenic differentiation.
29485180	4	27	theme	proliferative	815:827	arg1	capacity					829:836	proliferative capacity	815:836	proliferative capacity	815:836	Recently, a subpopulation of stem/progenitor cells has been identified within the auricular cartilage tissue, with similarities to MSCs in terms of proliferative capacity and cell surface biomarkers, but their potential for tissue engineering has not yet been explored.
29485180	4	28	from	MSCs	798:801	arg1	terms					806:810	terms	806:810	terms of proliferative capacity and cell surface biomarkers	806:864	Recently, a subpopulation of stem/progenitor cells has been identified within the auricular cartilage tissue, with similarities to MSCs in terms of proliferative capacity and cell surface biomarkers, but their potential for tissue engineering has not yet been explored.
29485180	5	29	theme	cells	1037:1041	arg1	ability					988:994	the in vitro cartilage-forming ability	957:994	the in vitro cartilage-forming ability of equine auricular cartilage progenitor cells (AuCPCs), bone marrow-derived MSCs and auricular chondrocytes in gelatin methacryloyl (gelMA)-based hydrogels	957:1151	This study compared the in vitro cartilage-forming ability of equine auricular cartilage progenitor cells (AuCPCs), bone marrow-derived MSCs and auricular chondrocytes in gelatin methacryloyl (gelMA)-based hydrogels over a period of 56 d, by assessing their ability to undergo chondrogenic differentiation.
29485180	2	30	theme	medicine	391:398	arg1	strategies					400:409	Current regenerative medicine strategies	370:409	Current regenerative medicine strategies	370:409	Current regenerative medicine strategies utilize chondrocytes from various locations or mesenchymal stromal cells (MSCs).
29485180	0	31	theme	Progenitor	0:9	arg1	cells					11:15	Progenitor cells	0:15	Progenitor cells in auricular cartilage	0:38	Progenitor cells in auricular cartilage demonstrate cartilage-forming capacity in 3D hydrogel culture.
29485180	2	32	theme	mesenchymal	458:468	arg1	MSCs					485:488	MSCs	485:488	MSCs	485:488	Current regenerative medicine strategies utilize chondrocytes from various locations or mesenchymal stromal cells (MSCs).
29485180	2	32	theme	mesenchymal	458:468	arg1	cells					478:482	mesenchymal stromal cells	458:482	mesenchymal stromal cells (MSCs)	458:489	Current regenerative medicine strategies utilize chondrocytes from various locations or mesenchymal stromal cells (MSCs).
29485180	5	33	theme	equine	999:1004	arg1	AuCPCs					1044:1049	AuCPCs	1044:1049	AuCPCs	1044:1049	This study compared the in vitro cartilage-forming ability of equine auricular cartilage progenitor cells (AuCPCs), bone marrow-derived MSCs and auricular chondrocytes in gelatin methacryloyl (gelMA)-based hydrogels over a period of 56 d, by assessing their ability to undergo chondrogenic differentiation.
29485180	5	33	theme	equine	999:1004	arg1	cells					1037:1041	equine auricular cartilage progenitor cells	999:1041	equine auricular cartilage progenitor cells (AuCPCs)	999:1050	This study compared the in vitro cartilage-forming ability of equine auricular cartilage progenitor cells (AuCPCs), bone marrow-derived MSCs and auricular chondrocytes in gelatin methacryloyl (gelMA)-based hydrogels over a period of 56 d, by assessing their ability to undergo chondrogenic differentiation.
29485180	9	34	theme	cell	1937:1940	arg1	source					1942:1947	an interesting cell source	1922:1947	an interesting cell source for the next generation of elastic cartilage tissue-engineered constructs	1922:2021	These results underscored the AuCPCs' tissue-specific differentiation potential, making them an interesting cell source for the next generation of elastic cartilage tissue-engineered constructs.
29485180	7	35	theme	matrix	1589:1594	arg1	production					1596:1605	matrix production	1589:1605	matrix production	1589:1605	Similar to MSCs and chondrocytes, AuCPCs displayed a marked ability to generate cartilaginous matrix, although, under the applied culture conditions, MSCs outperformed both cartilage-derived cell types in terms of matrix production and mechanical properties.
29485180	5	36	theme	marrow-derived	1058:1071	arg1	MSCs					1073:1076	bone marrow-derived MSCs	1053:1076	bone marrow-derived MSCs	1053:1076	This study compared the in vitro cartilage-forming ability of equine auricular cartilage progenitor cells (AuCPCs), bone marrow-derived MSCs and auricular chondrocytes in gelatin methacryloyl (gelMA)-based hydrogels over a period of 56 d, by assessing their ability to undergo chondrogenic differentiation.
29485180	8	37	theme	upregulated	1654:1664	arg1	expression					1671:1680	upregulated mRNA expression	1654:1680	upregulated mRNA expression of elastin	1654:1691	AuCPCs demonstrated upregulated mRNA expression of elastin, low expression of collagen type X and similar levels of proteoglycan production and mechanical properties as compared to chondrocytes.
29485180	0	38	theme	cartilage-forming	52:68	arg1	capacity					70:77	cartilage-forming capacity	52:77	cartilage-forming capacity in 3D hydrogel culture	52:100	Progenitor cells in auricular cartilage demonstrate cartilage-forming capacity in 3D hydrogel culture.
29485180	1	39	theme	capable	313:319	arg1	tissue					306:311	a tissue	304:311	a tissue capable of withstanding forces subjected to the auricle	304:367	Paramount for the generation of auricular structures of clinically-relevant size is the acquisition of a large number of cells maintaining an elastic cartilage phenotype, which is the key in producing a tissue capable of withstanding forces subjected to the auricle.
29485180	9	40	theme	next	1957:1960	arg1	generation					1962:1971	the next generation	1953:1971	the next generation of elastic cartilage tissue-engineered constructs	1953:2021	These results underscored the AuCPCs' tissue-specific differentiation potential, making them an interesting cell source for the next generation of elastic cartilage tissue-engineered constructs.
29485180	8	41	theme	elastin	1685:1691	arg1	levels					1740:1745	similar levels	1732:1745	similar levels of proteoglycan production and mechanical properties	1732:1798	AuCPCs demonstrated upregulated mRNA expression of elastin, low expression of collagen type X and similar levels of proteoglycan production and mechanical properties as compared to chondrocytes.
29485180	8	41	theme	elastin	1685:1691	arg1	expression					1698:1707	low expression	1694:1707	low expression of collagen type X	1694:1726	AuCPCs demonstrated upregulated mRNA expression of elastin, low expression of collagen type X and similar levels of proteoglycan production and mechanical properties as compared to chondrocytes.
29485180	8	41	theme	elastin	1685:1691	arg1	expression					1671:1680	upregulated mRNA expression	1654:1680	upregulated mRNA expression of elastin	1654:1691	AuCPCs demonstrated upregulated mRNA expression of elastin, low expression of collagen type X and similar levels of proteoglycan production and mechanical properties as compared to chondrocytes.
29485180	4	42	theme	surface	847:853	arg1	biomarkers					855:864	cell surface biomarkers	842:864	cell surface biomarkers	842:864	Recently, a subpopulation of stem/progenitor cells has been identified within the auricular cartilage tissue, with similarities to MSCs in terms of proliferative capacity and cell surface biomarkers, but their potential for tissue engineering has not yet been explored.
29485180	2	43	theme	Current	370:376	arg1	strategies					400:409	Current regenerative medicine strategies	370:409	Current regenerative medicine strategies	370:409	Current regenerative medicine strategies utilize chondrocytes from various locations or mesenchymal stromal cells (MSCs).
29485180	5	44	from	ability	988:994	arg1	hydrogels					1143:1151	gelatin methacryloyl (gelMA)-based hydrogels	1108:1151	gelatin methacryloyl (gelMA)-based hydrogels	1108:1151	This study compared the in vitro cartilage-forming ability of equine auricular cartilage progenitor cells (AuCPCs), bone marrow-derived MSCs and auricular chondrocytes in gelatin methacryloyl (gelMA)-based hydrogels over a period of 56 d, by assessing their ability to undergo chondrogenic differentiation.
29485180	1	45	theme	elastic	245:251	arg1	key					287:289	key	287:289	key	287:289	Paramount for the generation of auricular structures of clinically-relevant size is the acquisition of a large number of cells maintaining an elastic cartilage phenotype, which is the key in producing a tissue capable of withstanding forces subjected to the auricle.
29485180	1	45	theme	elastic	245:251	arg1	phenotype					263:271	an elastic cartilage phenotype	242:271	an elastic cartilage phenotype	242:271	Paramount for the generation of auricular structures of clinically-relevant size is the acquisition of a large number of cells maintaining an elastic cartilage phenotype, which is the key in producing a tissue capable of withstanding forces subjected to the auricle.
29485180	7	46	theme	cartilage-derived	1548:1564	arg1	types					1571:1575	both cartilage-derived cell types	1543:1575	both cartilage-derived cell types	1543:1575	Similar to MSCs and chondrocytes, AuCPCs displayed a marked ability to generate cartilaginous matrix, although, under the applied culture conditions, MSCs outperformed both cartilage-derived cell types in terms of matrix production and mechanical properties.
29485180	2	47	from	locations	445:453	arg1	chondrocytes					419:430	chondrocytes	419:430	chondrocytes from various locations or mesenchymal stromal cells (MSCs)	419:489	Current regenerative medicine strategies utilize chondrocytes from various locations or mesenchymal stromal cells (MSCs).
29485180	9	48	theme	cartilage	1984:1992	arg1	constructs					2012:2021	elastic cartilage tissue-engineered constructs	1976:2021	elastic cartilage tissue-engineered constructs	1976:2021	These results underscored the AuCPCs' tissue-specific differentiation potential, making them an interesting cell source for the next generation of elastic cartilage tissue-engineered constructs.
29485180	8	49	theme	low	1694:1696	arg1	expression					1698:1707	low expression	1694:1707	low expression of collagen type X	1694:1726	AuCPCs demonstrated upregulated mRNA expression of elastin, low expression of collagen type X and similar levels of proteoglycan production and mechanical properties as compared to chondrocytes.
29485180	8	50	theme	proteoglycan	1750:1761	arg1	production					1763:1772	proteoglycan production	1750:1772	proteoglycan production	1750:1772	AuCPCs demonstrated upregulated mRNA expression of elastin, low expression of collagen type X and similar levels of proteoglycan production and mechanical properties as compared to chondrocytes.
29485180	9	51	theme	constructs	2012:2021	arg1	generation					1962:1971	the next generation	1953:1971	the next generation of elastic cartilage tissue-engineered constructs	1953:2021	These results underscored the AuCPCs' tissue-specific differentiation potential, making them an interesting cell source for the next generation of elastic cartilage tissue-engineered constructs.
29485180	7	52	theme	applied	1497:1503	arg1	conditions					1513:1522	the applied culture conditions	1493:1522	the applied culture conditions	1493:1522	Similar to MSCs and chondrocytes, AuCPCs displayed a marked ability to generate cartilaginous matrix, although, under the applied culture conditions, MSCs outperformed both cartilage-derived cell types in terms of matrix production and mechanical properties.
29485180	8	53	theme	type	1721:1724	arg1	X					1726:1726	collagen type X	1712:1726	collagen type X	1712:1726	AuCPCs demonstrated upregulated mRNA expression of elastin, low expression of collagen type X and similar levels of proteoglycan production and mechanical properties as compared to chondrocytes.
29485180	6	54	theme	compression	1315:1325	arg1	testing					1327:1333	compression testing	1315:1333	compression testing	1315:1333	Neocartilage formation was assessed through gene expression profiling, compression testing, biochemical composition and histology.
29485180	8	55	theme	properties	1789:1798	arg1	levels					1740:1745	similar levels	1732:1745	similar levels of proteoglycan production and mechanical properties	1732:1798	AuCPCs demonstrated upregulated mRNA expression of elastin, low expression of collagen type X and similar levels of proteoglycan production and mechanical properties as compared to chondrocytes.
29485180	8	55	theme	properties	1789:1798	arg1	expression					1698:1707	low expression	1694:1707	low expression of collagen type X	1694:1726	AuCPCs demonstrated upregulated mRNA expression of elastin, low expression of collagen type X and similar levels of proteoglycan production and mechanical properties as compared to chondrocytes.
29485180	8	55	theme	properties	1789:1798	arg1	expression					1671:1680	upregulated mRNA expression	1654:1680	upregulated mRNA expression of elastin	1654:1691	AuCPCs demonstrated upregulated mRNA expression of elastin, low expression of collagen type X and similar levels of proteoglycan production and mechanical properties as compared to chondrocytes.
29485180	7	56	theme	culture	1505:1511	arg1	conditions					1513:1522	the applied culture conditions	1493:1522	the applied culture conditions	1493:1522	Similar to MSCs and chondrocytes, AuCPCs displayed a marked ability to generate cartilaginous matrix, although, under the applied culture conditions, MSCs outperformed both cartilage-derived cell types in terms of matrix production and mechanical properties.
29485180	2	57	from	cells	478:482	arg1	chondrocytes					419:430	chondrocytes	419:430	chondrocytes from various locations or mesenchymal stromal cells (MSCs)	419:489	Current regenerative medicine strategies utilize chondrocytes from various locations or mesenchymal stromal cells (MSCs).
29485180	4	58	theme	tissue	891:896	arg1	engineering					898:908	tissue engineering	891:908	tissue engineering	891:908	Recently, a subpopulation of stem/progenitor cells has been identified within the auricular cartilage tissue, with similarities to MSCs in terms of proliferative capacity and cell surface biomarkers, but their potential for tissue engineering has not yet been explored.
29485180	4	59	theme	cells	712:716	arg1	subpopulation					679:691	a subpopulation	677:691	a subpopulation of stem/progenitor cells	677:716	Recently, a subpopulation of stem/progenitor cells has been identified within the auricular cartilage tissue, with similarities to MSCs in terms of proliferative capacity and cell surface biomarkers, but their potential for tissue engineering has not yet been explored.
29485180	1	60	theme	large	208:212	arg1	number					214:219	a large number	206:219	a large number of cells maintaining an elastic cartilage phenotype, which is the key in producing a tissue capable of withstanding forces subjected to the auricle	206:367	Paramount for the generation of auricular structures of clinically-relevant size is the acquisition of a large number of cells maintaining an elastic cartilage phenotype, which is the key in producing a tissue capable of withstanding forces subjected to the auricle.
29485180	7	61	link	cartilage-derived	1548:1564	arg1	types					1571:1575	both cartilage-derived cell types	1543:1575	both cartilage-derived cell types	1543:1575	Similar to MSCs and chondrocytes, AuCPCs displayed a marked ability to generate cartilaginous matrix, although, under the applied culture conditions, MSCs outperformed both cartilage-derived cell types in terms of matrix production and mechanical properties.
29485180	3	62	theme	chondrogenic	593:604	arg1	differentiation					606:620	inefficient chondrogenic differentiation	581:620	inefficient chondrogenic differentiation	581:620	However, the quality of neo-tissues resulting from these cell types is inadequate due to inefficient chondrogenic differentiation and endochondral ossification, respectively.
29485180	5	63	link	marrow-derived	1058:1071	arg1	MSCs					1073:1076	bone marrow-derived MSCs	1053:1076	bone marrow-derived MSCs	1053:1076	This study compared the in vitro cartilage-forming ability of equine auricular cartilage progenitor cells (AuCPCs), bone marrow-derived MSCs and auricular chondrocytes in gelatin methacryloyl (gelMA)-based hydrogels over a period of 56 d, by assessing their ability to undergo chondrogenic differentiation.
29485180	4	64	theme	auricular	749:757	arg1	tissue					769:774	the auricular cartilage tissue	745:774	the auricular cartilage tissue	745:774	Recently, a subpopulation of stem/progenitor cells has been identified within the auricular cartilage tissue, with similarities to MSCs in terms of proliferative capacity and cell surface biomarkers, but their potential for tissue engineering has not yet been explored.
29485180	5	65	theme	chondrocytes	1092:1103	arg1	ability					988:994	the in vitro cartilage-forming ability	957:994	the in vitro cartilage-forming ability of equine auricular cartilage progenitor cells (AuCPCs), bone marrow-derived MSCs and auricular chondrocytes in gelatin methacryloyl (gelMA)-based hydrogels	957:1151	This study compared the in vitro cartilage-forming ability of equine auricular cartilage progenitor cells (AuCPCs), bone marrow-derived MSCs and auricular chondrocytes in gelatin methacryloyl (gelMA)-based hydrogels over a period of 56 d, by assessing their ability to undergo chondrogenic differentiation.
29485180	1	66	theme	auricular	135:143	arg1	structures					145:154	auricular structures	135:154	auricular structures of clinically-relevant size	135:182	Paramount for the generation of auricular structures of clinically-relevant size is the acquisition of a large number of cells maintaining an elastic cartilage phenotype, which is the key in producing a tissue capable of withstanding forces subjected to the auricle.
29485180	6	67	theme	Neocartilage	1244:1255	arg1	formation					1257:1265	Neocartilage formation	1244:1265	Neocartilage formation	1244:1265	Neocartilage formation was assessed through gene expression profiling, compression testing, biochemical composition and histology.
29485180	0	68	theme	3D	82:83	arg1	culture					94:100	3D hydrogel culture	82:100	3D hydrogel culture	82:100	Progenitor cells in auricular cartilage demonstrate cartilage-forming capacity in 3D hydrogel culture.
29485180	1	69	theme	size	179:182	arg1	structures					145:154	auricular structures	135:154	auricular structures of clinically-relevant size	135:182	Paramount for the generation of auricular structures of clinically-relevant size is the acquisition of a large number of cells maintaining an elastic cartilage phenotype, which is the key in producing a tissue capable of withstanding forces subjected to the auricle.
29485180	0	70	from	cells	11:15	arg1	cartilage					30:38	auricular cartilage	20:38	auricular cartilage	20:38	Progenitor cells in auricular cartilage demonstrate cartilage-forming capacity in 3D hydrogel culture.
29485180	5	71	theme	d	1173:1173	arg1	period					1160:1165	a period	1158:1165	a period of 56 d	1158:1173	This study compared the in vitro cartilage-forming ability of equine auricular cartilage progenitor cells (AuCPCs), bone marrow-derived MSCs and auricular chondrocytes in gelatin methacryloyl (gelMA)-based hydrogels over a period of 56 d, by assessing their ability to undergo chondrogenic differentiation.
29485180	5	72	theme	auricular	1006:1014	arg1	AuCPCs					1044:1049	AuCPCs	1044:1049	AuCPCs	1044:1049	This study compared the in vitro cartilage-forming ability of equine auricular cartilage progenitor cells (AuCPCs), bone marrow-derived MSCs and auricular chondrocytes in gelatin methacryloyl (gelMA)-based hydrogels over a period of 56 d, by assessing their ability to undergo chondrogenic differentiation.
29485180	5	72	theme	auricular	1006:1014	arg1	cells					1037:1041	equine auricular cartilage progenitor cells	999:1041	equine auricular cartilage progenitor cells (AuCPCs)	999:1050	This study compared the in vitro cartilage-forming ability of equine auricular cartilage progenitor cells (AuCPCs), bone marrow-derived MSCs and auricular chondrocytes in gelatin methacryloyl (gelMA)-based hydrogels over a period of 56 d, by assessing their ability to undergo chondrogenic differentiation.
29485180	7	73	theme	properties	1622:1631	arg1	terms					1580:1584	terms	1580:1584	terms of matrix production and mechanical properties	1580:1631	Similar to MSCs and chondrocytes, AuCPCs displayed a marked ability to generate cartilaginous matrix, although, under the applied culture conditions, MSCs outperformed both cartilage-derived cell types in terms of matrix production and mechanical properties.
29485180	9	74	theme	differentiation	1883:1897	arg1	potential					1899:1907	the AuCPCs' tissue-specific differentiation potential	1855:1907	the AuCPCs' tissue-specific differentiation potential	1855:1907	These results underscored the AuCPCs' tissue-specific differentiation potential, making them an interesting cell source for the next generation of elastic cartilage tissue-engineered constructs.
29485180	0	75	from	capacity	70:77	arg1	culture					94:100	3D hydrogel culture	82:100	3D hydrogel culture	82:100	Progenitor cells in auricular cartilage demonstrate cartilage-forming capacity in 3D hydrogel culture.
29485180	5	76	theme	progenitor	1026:1035	arg1	AuCPCs					1044:1049	AuCPCs	1044:1049	AuCPCs	1044:1049	This study compared the in vitro cartilage-forming ability of equine auricular cartilage progenitor cells (AuCPCs), bone marrow-derived MSCs and auricular chondrocytes in gelatin methacryloyl (gelMA)-based hydrogels over a period of 56 d, by assessing their ability to undergo chondrogenic differentiation.
29485180	5	76	theme	progenitor	1026:1035	arg1	cells					1037:1041	equine auricular cartilage progenitor cells	999:1041	equine auricular cartilage progenitor cells (AuCPCs)	999:1050	This study compared the in vitro cartilage-forming ability of equine auricular cartilage progenitor cells (AuCPCs), bone marrow-derived MSCs and auricular chondrocytes in gelatin methacryloyl (gelMA)-based hydrogels over a period of 56 d, by assessing their ability to undergo chondrogenic differentiation.
29485180	5	77	theme	cartilage-forming	970:986	arg1	ability					988:994	the in vitro cartilage-forming ability	957:994	the in vitro cartilage-forming ability of equine auricular cartilage progenitor cells (AuCPCs), bone marrow-derived MSCs and auricular chondrocytes in gelatin methacryloyl (gelMA)-based hydrogels	957:1151	This study compared the in vitro cartilage-forming ability of equine auricular cartilage progenitor cells (AuCPCs), bone marrow-derived MSCs and auricular chondrocytes in gelatin methacryloyl (gelMA)-based hydrogels over a period of 56 d, by assessing their ability to undergo chondrogenic differentiation.
29485180	2	78	theme	stromal	470:476	arg1	MSCs					485:488	MSCs	485:488	MSCs	485:488	Current regenerative medicine strategies utilize chondrocytes from various locations or mesenchymal stromal cells (MSCs).
29485180	2	78	theme	stromal	470:476	arg1	cells					478:482	mesenchymal stromal cells	458:482	mesenchymal stromal cells (MSCs)	458:489	Current regenerative medicine strategies utilize chondrocytes from various locations or mesenchymal stromal cells (MSCs).
29485180	2	79	theme	regenerative	378:389	arg1	strategies					400:409	Current regenerative medicine strategies	370:409	Current regenerative medicine strategies	370:409	Current regenerative medicine strategies utilize chondrocytes from various locations or mesenchymal stromal cells (MSCs).
29485180	0	80	theme	auricular	20:28	arg1	cartilage					30:38	auricular cartilage	20:38	auricular cartilage	20:38	Progenitor cells in auricular cartilage demonstrate cartilage-forming capacity in 3D hydrogel culture.
29485180	9	81	theme	interesting	1925:1935	arg1	source					1942:1947	an interesting cell source	1922:1947	an interesting cell source for the next generation of elastic cartilage tissue-engineered constructs	1922:2021	These results underscored the AuCPCs' tissue-specific differentiation potential, making them an interesting cell source for the next generation of elastic cartilage tissue-engineered constructs.
29485180	2	82	theme	various	437:443	arg1	locations					445:453	various locations	437:453	various locations	437:453	Current regenerative medicine strategies utilize chondrocytes from various locations or mesenchymal stromal cells (MSCs).
29485180	5	83	theme	chondrogenic	1214:1225	arg1	differentiation					1227:1241	chondrogenic differentiation	1214:1241	chondrogenic differentiation	1214:1241	This study compared the in vitro cartilage-forming ability of equine auricular cartilage progenitor cells (AuCPCs), bone marrow-derived MSCs and auricular chondrocytes in gelatin methacryloyl (gelMA)-based hydrogels over a period of 56 d, by assessing their ability to undergo chondrogenic differentiation.
29485180	7	84	theme	mechanical	1611:1620	arg1	properties					1622:1631	mechanical properties	1611:1631	mechanical properties	1611:1631	Similar to MSCs and chondrocytes, AuCPCs displayed a marked ability to generate cartilaginous matrix, although, under the applied culture conditions, MSCs outperformed both cartilage-derived cell types in terms of matrix production and mechanical properties.
29485180	8	85	theme	mRNA	1666:1669	arg1	expression					1671:1680	upregulated mRNA expression	1654:1680	upregulated mRNA expression of elastin	1654:1691	AuCPCs demonstrated upregulated mRNA expression of elastin, low expression of collagen type X and similar levels of proteoglycan production and mechanical properties as compared to chondrocytes.
29485180	4	86	theme	biomarkers	855:864	arg1	terms					806:810	terms	806:810	terms of proliferative capacity and cell surface biomarkers	806:864	Recently, a subpopulation of stem/progenitor cells has been identified within the auricular cartilage tissue, with similarities to MSCs in terms of proliferative capacity and cell surface biomarkers, but their potential for tissue engineering has not yet been explored.
29485180	7	87	theme	production	1596:1605	arg1	terms					1580:1584	terms	1580:1584	terms of matrix production and mechanical properties	1580:1631	Similar to MSCs and chondrocytes, AuCPCs displayed a marked ability to generate cartilaginous matrix, although, under the applied culture conditions, MSCs outperformed both cartilage-derived cell types in terms of matrix production and mechanical properties.
29485180	5	88	theme	MSCs	1073:1076	arg1	ability					988:994	the in vitro cartilage-forming ability	957:994	the in vitro cartilage-forming ability of equine auricular cartilage progenitor cells (AuCPCs), bone marrow-derived MSCs and auricular chondrocytes in gelatin methacryloyl (gelMA)-based hydrogels	957:1151	This study compared the in vitro cartilage-forming ability of equine auricular cartilage progenitor cells (AuCPCs), bone marrow-derived MSCs and auricular chondrocytes in gelatin methacryloyl (gelMA)-based hydrogels over a period of 56 d, by assessing their ability to undergo chondrogenic differentiation.
29485180	8	89	theme	similar	1732:1738	arg1	levels					1740:1745	similar levels	1732:1745	similar levels of proteoglycan production and mechanical properties	1732:1798	AuCPCs demonstrated upregulated mRNA expression of elastin, low expression of collagen type X and similar levels of proteoglycan production and mechanical properties as compared to chondrocytes.
29485180	4	90	theme	capacity	829:836	arg1	terms					806:810	terms	806:810	terms of proliferative capacity and cell surface biomarkers	806:864	Recently, a subpopulation of stem/progenitor cells has been identified within the auricular cartilage tissue, with similarities to MSCs in terms of proliferative capacity and cell surface biomarkers, but their potential for tissue engineering has not yet been explored.
29485180	9	91	theme	elastic	1976:1982	arg1	constructs					2012:2021	elastic cartilage tissue-engineered constructs	1976:2021	elastic cartilage tissue-engineered constructs	1976:2021	These results underscored the AuCPCs' tissue-specific differentiation potential, making them an interesting cell source for the next generation of elastic cartilage tissue-engineered constructs.
29485180	4	92	theme	cell	842:845	arg1	biomarkers					855:864	cell surface biomarkers	842:864	cell surface biomarkers	842:864	Recently, a subpopulation of stem/progenitor cells has been identified within the auricular cartilage tissue, with similarities to MSCs in terms of proliferative capacity and cell surface biomarkers, but their potential for tissue engineering has not yet been explored.
29485180	8	93	theme	production	1763:1772	arg1	levels					1740:1745	similar levels	1732:1745	similar levels of proteoglycan production and mechanical properties	1732:1798	AuCPCs demonstrated upregulated mRNA expression of elastin, low expression of collagen type X and similar levels of proteoglycan production and mechanical properties as compared to chondrocytes.
29485180	8	93	theme	production	1763:1772	arg1	expression					1698:1707	low expression	1694:1707	low expression of collagen type X	1694:1726	AuCPCs demonstrated upregulated mRNA expression of elastin, low expression of collagen type X and similar levels of proteoglycan production and mechanical properties as compared to chondrocytes.
29485180	8	93	theme	production	1763:1772	arg1	expression					1671:1680	upregulated mRNA expression	1654:1680	upregulated mRNA expression of elastin	1654:1691	AuCPCs demonstrated upregulated mRNA expression of elastin, low expression of collagen type X and similar levels of proteoglycan production and mechanical properties as compared to chondrocytes.
29485180	1	94	theme	cartilage	253:261	arg1	key					287:289	key	287:289	key	287:289	Paramount for the generation of auricular structures of clinically-relevant size is the acquisition of a large number of cells maintaining an elastic cartilage phenotype, which is the key in producing a tissue capable of withstanding forces subjected to the auricle.
29485180	1	94	theme	cartilage	253:261	arg1	phenotype					263:271	an elastic cartilage phenotype	242:271	an elastic cartilage phenotype	242:271	Paramount for the generation of auricular structures of clinically-relevant size is the acquisition of a large number of cells maintaining an elastic cartilage phenotype, which is the key in producing a tissue capable of withstanding forces subjected to the auricle.
30267782	6	0	from	hydrogel	870:877	arg1	ink					894:896	the printer ink	882:896	the printer ink	882:896	Increasing the amount of hydroxypropyl methylcellulose hydrogel in the printer ink led to delayed drug dissolution, decreased weight, and decreased hardness of the tablets.
30267782	7	1	theme	powder	1089:1094	arg1	tablets					1108:1114	powder compression tablets	1089:1114	powder compression tablets	1089:1114	Delayed drug dissolution was also observed when the amount of disintegrating agent typically used in powder compression tablets was increased in the ink, and increasing the incorporated amount of the disintegrating agent crospovidone increased the hardness of the tablets.
30267782	7	2	theme	crospovidone	1209:1220	arg1	crospovidone					1209:1220	the disintegrating agent crospovidone	1184:1220	the disintegrating agent crospovidone	1184:1220	Delayed drug dissolution was also observed when the amount of disintegrating agent typically used in powder compression tablets was increased in the ink, and increasing the incorporated amount of the disintegrating agent crospovidone increased the hardness of the tablets.
30267782	7	2	theme	crospovidone	1209:1220	arg1	amount					1174:1179	the incorporated amount	1157:1179	the incorporated amount of the disintegrating agent crospovidone	1157:1220	Delayed drug dissolution was also observed when the amount of disintegrating agent typically used in powder compression tablets was increased in the ink, and increasing the incorporated amount of the disintegrating agent crospovidone increased the hardness of the tablets.
30267782	6	3	from	ink	894:896	arg1	hydrogel					870:877	hydroxypropyl methylcellulose hydrogel	840:877	hydroxypropyl methylcellulose hydrogel in the printer ink	840:896	Increasing the amount of hydroxypropyl methylcellulose hydrogel in the printer ink led to delayed drug dissolution, decreased weight, and decreased hardness of the tablets.
30267782	6	3	from	ink	894:896	arg1	amount					830:835	the amount	826:835	the amount of hydroxypropyl methylcellulose hydrogel in the printer ink	826:896	Increasing the amount of hydroxypropyl methylcellulose hydrogel in the printer ink led to delayed drug dissolution, decreased weight, and decreased hardness of the tablets.
30267782	6	4	theme	printer	886:892	arg1	ink					894:896	the printer ink	882:896	the printer ink	882:896	Increasing the amount of hydroxypropyl methylcellulose hydrogel in the printer ink led to delayed drug dissolution, decreased weight, and decreased hardness of the tablets.
30267782	7	5	theme	compression	1096:1106	arg1	tablets					1108:1114	powder compression tablets	1089:1114	powder compression tablets	1089:1114	Delayed drug dissolution was also observed when the amount of disintegrating agent typically used in powder compression tablets was increased in the ink, and increasing the incorporated amount of the disintegrating agent crospovidone increased the hardness of the tablets.
30267782	2	6	theme	3D	356:357	arg1	bioprinter					359:368	a semisolid extrusion-type 3D bioprinter	329:368	a semisolid extrusion-type 3D bioprinter applicable for tissue engineering	329:402	Here, we prepared tablets containing naftopidil as a model drug using a semisolid extrusion-type 3D bioprinter applicable for tissue engineering.
30267782	7	7	theme	incorporated	1161:1172	arg1	crospovidone					1209:1220	the disintegrating agent crospovidone	1184:1220	the disintegrating agent crospovidone	1184:1220	Delayed drug dissolution was also observed when the amount of disintegrating agent typically used in powder compression tablets was increased in the ink, and increasing the incorporated amount of the disintegrating agent crospovidone increased the hardness of the tablets.
30267782	7	7	theme	incorporated	1161:1172	arg1	amount					1174:1179	the incorporated amount	1157:1179	the incorporated amount of the disintegrating agent crospovidone	1157:1220	Delayed drug dissolution was also observed when the amount of disintegrating agent typically used in powder compression tablets was increased in the ink, and increasing the incorporated amount of the disintegrating agent crospovidone increased the hardness of the tablets.
30267782	4	8	theme	gel	622:624	arg1	product					626:632	The resulting 3D-printed gel product	597:632	The resulting 3D-printed gel product	597:632	The resulting 3D-printed gel product was dried to obtain tablets.
30267782	8	9	theme	extrusion-type	1352:1365	arg1	printers					1370:1377	semisolid extrusion-type 3D printers	1342:1377	semisolid extrusion-type 3D printers	1342:1377	Our results will provide useful information for the preparation of tablets using semisolid extrusion-type 3D printers.
30267782	7	10	theme	Delayed	988:994	arg1	dissolution					1001:1011	Delayed drug dissolution	988:1011	Delayed drug dissolution	988:1011	Delayed drug dissolution was also observed when the amount of disintegrating agent typically used in powder compression tablets was increased in the ink, and increasing the incorporated amount of the disintegrating agent crospovidone increased the hardness of the tablets.
30267782	0	11	theme	Printed	116:122	arg1	Hydrogel					124:131	the Printed Hydrogel and Resulting Tablets	112:153	Hydrogel	124:131	Fabrication of Naftopidil-Loaded Tablets Using a Semisolid Extrusion-Type 3D Printer and the Characteristics of the Printed Hydrogel and Resulting Tablets.
30267782	6	12	theme	hydrogel	870:877	arg1	hydrogel					870:877	hydroxypropyl methylcellulose hydrogel	840:877	hydroxypropyl methylcellulose hydrogel in the printer ink	840:896	Increasing the amount of hydroxypropyl methylcellulose hydrogel in the printer ink led to delayed drug dissolution, decreased weight, and decreased hardness of the tablets.
30267782	6	12	theme	hydrogel	870:877	arg1	amount					830:835	the amount	826:835	the amount of hydroxypropyl methylcellulose hydrogel in the printer ink	826:896	Increasing the amount of hydroxypropyl methylcellulose hydrogel in the printer ink led to delayed drug dissolution, decreased weight, and decreased hardness of the tablets.
30267782	7	13	theme	agent	1065:1069	arg1	agent					1065:1069	disintegrating agent	1050:1069	disintegrating agent typically used in powder compression tablets	1050:1114	Delayed drug dissolution was also observed when the amount of disintegrating agent typically used in powder compression tablets was increased in the ink, and increasing the incorporated amount of the disintegrating agent crospovidone increased the hardness of the tablets.
30267782	7	13	theme	agent	1065:1069	arg1	amount					1040:1045	the amount	1036:1045	the amount of disintegrating agent typically used in powder compression tablets	1036:1114	Delayed drug dissolution was also observed when the amount of disintegrating agent typically used in powder compression tablets was increased in the ink, and increasing the incorporated amount of the disintegrating agent crospovidone increased the hardness of the tablets.
30267782	5	14	theme	several	793:799	arg1	formulations					801:812	several formulations	793:812	several formulations	793:812	The rheological properties of the printer ink changed as its composition was changed, and tablets were prepared successfully from several formulations.
30267782	3	15	theme	hydrogel	542:549	arg1	hydrogel					542:549	hydroxypropyl methylcellulose hydrogel	512:549	hydroxypropyl methylcellulose hydrogel	512:549	A hydrogel is typically used as the printer ink for 3D bioprinters, and we incorporated various amounts of hydroxypropyl methylcellulose hydrogel (30%, 40%, and 50% gel) into the printer ink.
30267782	3	15	theme	hydrogel	542:549	arg1	ink					449:451	the printer ink	437:451	the printer ink for 3D bioprinters	437:470	A hydrogel is typically used as the printer ink for 3D bioprinters, and we incorporated various amounts of hydroxypropyl methylcellulose hydrogel (30%, 40%, and 50% gel) into the printer ink.
30267782	3	15	theme	hydrogel	542:549	arg1	hydrogel					407:414	A hydrogel	405:414	A hydrogel	405:414	A hydrogel is typically used as the printer ink for 3D bioprinters, and we incorporated various amounts of hydroxypropyl methylcellulose hydrogel (30%, 40%, and 50% gel) into the printer ink.
30267782	3	15	theme	hydrogel	542:549	arg1	amounts					501:507	various amounts	493:507	various amounts of hydroxypropyl methylcellulose hydrogel (30%, 40%, and 50% gel)	493:573	A hydrogel is typically used as the printer ink for 3D bioprinters, and we incorporated various amounts of hydroxypropyl methylcellulose hydrogel (30%, 40%, and 50% gel) into the printer ink.
30267782	1	16	theme	three-dimensional	174:190	arg1	drugs					205:209	three-dimensional (3D)-printed drugs	174:209	three-dimensional (3D)-printed drugs	174:209	The production of three-dimensional (3D)-printed drugs holds promise for future personalized medicine.
30267782	5	17	theme	ink	705:707	arg1	properties					679:688	The rheological properties	663:688	The rheological properties of the printer ink	663:707	The rheological properties of the printer ink changed as its composition was changed, and tablets were prepared successfully from several formulations.
30267782	6	18	theme	methylcellulose	854:868	arg1	hydrogel					870:877	hydroxypropyl methylcellulose hydrogel	840:877	hydroxypropyl methylcellulose hydrogel in the printer ink	840:896	Increasing the amount of hydroxypropyl methylcellulose hydrogel in the printer ink led to delayed drug dissolution, decreased weight, and decreased hardness of the tablets.
30267782	8	19	theme	tablets	1328:1334	arg1	preparation					1313:1323	the preparation	1309:1323	the preparation of tablets using semisolid extrusion-type 3D printers	1309:1377	Our results will provide useful information for the preparation of tablets using semisolid extrusion-type 3D printers.
30267782	4	20	theme	3D-printed	611:620	arg1	product					626:632	The resulting 3D-printed gel product	597:632	The resulting 3D-printed gel product	597:632	The resulting 3D-printed gel product was dried to obtain tablets.
30267782	6	21	theme	hydroxypropyl	840:852	arg1	hydrogel					870:877	hydroxypropyl methylcellulose hydrogel	840:877	hydroxypropyl methylcellulose hydrogel in the printer ink	840:896	Increasing the amount of hydroxypropyl methylcellulose hydrogel in the printer ink led to delayed drug dissolution, decreased weight, and decreased hardness of the tablets.
30267782	3	22	dep	amounts	501:507	arg1	%					554:554	30%	552:554	30%	552:554	A hydrogel is typically used as the printer ink for 3D bioprinters, and we incorporated various amounts of hydroxypropyl methylcellulose hydrogel (30%, 40%, and 50% gel) into the printer ink.
30267782	3	22	dep	amounts	501:507	arg1	gel					570:572	50% gel	566:572	50% gel	566:572	A hydrogel is typically used as the printer ink for 3D bioprinters, and we incorporated various amounts of hydroxypropyl methylcellulose hydrogel (30%, 40%, and 50% gel) into the printer ink.
30267782	3	22	dep	amounts	501:507	arg1	%					559:559	40%	557:559	40%	557:559	A hydrogel is typically used as the printer ink for 3D bioprinters, and we incorporated various amounts of hydroxypropyl methylcellulose hydrogel (30%, 40%, and 50% gel) into the printer ink.
30267782	5	23	theme	rheological	667:677	arg1	properties					679:688	The rheological properties	663:688	The rheological properties of the printer ink	663:707	The rheological properties of the printer ink changed as its composition was changed, and tablets were prepared successfully from several formulations.
30267782	7	24	theme	disintegrating	1050:1063	arg1	agent					1065:1069	disintegrating agent	1050:1069	disintegrating agent typically used in powder compression tablets	1050:1114	Delayed drug dissolution was also observed when the amount of disintegrating agent typically used in powder compression tablets was increased in the ink, and increasing the incorporated amount of the disintegrating agent crospovidone increased the hardness of the tablets.
30267782	2	25	theme	model	312:316	arg1	naftopidil					296:305	naftopidil	296:305	naftopidil	296:305	Here, we prepared tablets containing naftopidil as a model drug using a semisolid extrusion-type 3D bioprinter applicable for tissue engineering.
30267782	2	25	theme	model	312:316	arg1	drug					318:321	a model drug	310:321	a model drug using a semisolid extrusion-type 3D bioprinter applicable for tissue engineering	310:402	Here, we prepared tablets containing naftopidil as a model drug using a semisolid extrusion-type 3D bioprinter applicable for tissue engineering.
30267782	2	26	theme	tissue	385:390	arg1	engineering					392:402	tissue engineering	385:402	tissue engineering	385:402	Here, we prepared tablets containing naftopidil as a model drug using a semisolid extrusion-type 3D bioprinter applicable for tissue engineering.
30267782	0	27	theme	Tablets	33:39	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of Naftopidil-Loaded Tablets Using a Semisolid Extrusion-Type 3D Printer and the Characteristics of the Printed Hydrogel and Resulting Tablets.	0:154	Fabrication of Naftopidil-Loaded Tablets Using a Semisolid Extrusion-Type 3D Printer and the Characteristics of the Printed Hydrogel and Resulting Tablets.
30267782	1	28	theme	-printed	196:203	arg1	drugs					205:209	three-dimensional (3D)-printed drugs	174:209	three-dimensional (3D)-printed drugs	174:209	The production of three-dimensional (3D)-printed drugs holds promise for future personalized medicine.
30267782	0	29	theme	Naftopidil-Loaded	15:31	arg1	Tablets					33:39	Naftopidil-Loaded Tablets	15:39	Naftopidil-Loaded Tablets	15:39	Fabrication of Naftopidil-Loaded Tablets Using a Semisolid Extrusion-Type 3D Printer and the Characteristics of the Printed Hydrogel and Resulting Tablets.
30267782	2	30	contain	containing	285:294	arg2	drug					318:321	a model drug	310:321	a model drug using a semisolid extrusion-type 3D bioprinter applicable for tissue engineering	310:402	Here, we prepared tablets containing naftopidil as a model drug using a semisolid extrusion-type 3D bioprinter applicable for tissue engineering.
30267782	2	30	contain	containing	285:294	arg2	naftopidil					296:305	naftopidil	296:305	naftopidil	296:305	Here, we prepared tablets containing naftopidil as a model drug using a semisolid extrusion-type 3D bioprinter applicable for tissue engineering.
30267782	2	30	contain	containing	285:294	arg1	tablets					277:283	tablets	277:283	tablets containing naftopidil as a model drug using a semisolid extrusion-type 3D bioprinter applicable for tissue engineering	277:402	Here, we prepared tablets containing naftopidil as a model drug using a semisolid extrusion-type 3D bioprinter applicable for tissue engineering.
30267782	0	31	theme	Hydrogel	124:131	arg1	Printer					77:83	a Semisolid Extrusion-Type 3D Printer	47:83	a Semisolid Extrusion-Type 3D Printer	47:83	Fabrication of Naftopidil-Loaded Tablets Using a Semisolid Extrusion-Type 3D Printer and the Characteristics of the Printed Hydrogel and Resulting Tablets.
30267782	0	31	theme	Hydrogel	124:131	arg1	Characteristics					93:107	the Characteristics	89:107	the Characteristics of the Printed Hydrogel and Resulting Tablets	89:153	Fabrication of Naftopidil-Loaded Tablets Using a Semisolid Extrusion-Type 3D Printer and the Characteristics of the Printed Hydrogel and Resulting Tablets.
30267782	8	32	theme	3D	1367:1368	arg1	printers					1370:1377	semisolid extrusion-type 3D printers	1342:1377	semisolid extrusion-type 3D printers	1342:1377	Our results will provide useful information for the preparation of tablets using semisolid extrusion-type 3D printers.
30267782	1	33	theme	drugs	205:209	arg1	production					160:169	The production	156:169	The production of three-dimensional (3D)-printed drugs	156:209	The production of three-dimensional (3D)-printed drugs holds promise for future personalized medicine.
30267782	3	34	theme	various	493:499	arg1	hydrogel					542:549	hydroxypropyl methylcellulose hydrogel	512:549	hydroxypropyl methylcellulose hydrogel	512:549	A hydrogel is typically used as the printer ink for 3D bioprinters, and we incorporated various amounts of hydroxypropyl methylcellulose hydrogel (30%, 40%, and 50% gel) into the printer ink.
30267782	3	34	theme	various	493:499	arg1	ink					449:451	the printer ink	437:451	the printer ink for 3D bioprinters	437:470	A hydrogel is typically used as the printer ink for 3D bioprinters, and we incorporated various amounts of hydroxypropyl methylcellulose hydrogel (30%, 40%, and 50% gel) into the printer ink.
30267782	3	34	theme	various	493:499	arg1	hydrogel					407:414	A hydrogel	405:414	A hydrogel	405:414	A hydrogel is typically used as the printer ink for 3D bioprinters, and we incorporated various amounts of hydroxypropyl methylcellulose hydrogel (30%, 40%, and 50% gel) into the printer ink.
30267782	3	34	theme	various	493:499	arg1	amounts					501:507	various amounts	493:507	various amounts of hydroxypropyl methylcellulose hydrogel (30%, 40%, and 50% gel)	493:573	A hydrogel is typically used as the printer ink for 3D bioprinters, and we incorporated various amounts of hydroxypropyl methylcellulose hydrogel (30%, 40%, and 50% gel) into the printer ink.
30267782	0	35	theme	Tablets	147:153	arg1	Printer					77:83	a Semisolid Extrusion-Type 3D Printer	47:83	a Semisolid Extrusion-Type 3D Printer	47:83	Fabrication of Naftopidil-Loaded Tablets Using a Semisolid Extrusion-Type 3D Printer and the Characteristics of the Printed Hydrogel and Resulting Tablets.
30267782	0	35	theme	Tablets	147:153	arg1	Characteristics					93:107	the Characteristics	89:107	the Characteristics of the Printed Hydrogel and Resulting Tablets	89:153	Fabrication of Naftopidil-Loaded Tablets Using a Semisolid Extrusion-Type 3D Printer and the Characteristics of the Printed Hydrogel and Resulting Tablets.
30267782	3	36	theme	%	568:568	arg1	gel					570:572	50% gel	566:572	50% gel	566:572	A hydrogel is typically used as the printer ink for 3D bioprinters, and we incorporated various amounts of hydroxypropyl methylcellulose hydrogel (30%, 40%, and 50% gel) into the printer ink.
30267782	4	37	theme	resulting	601:609	arg1	product					626:632	The resulting 3D-printed gel product	597:632	The resulting 3D-printed gel product	597:632	The resulting 3D-printed gel product was dried to obtain tablets.
30267782	3	38	theme	printer	441:447	arg1	hydrogel					542:549	hydroxypropyl methylcellulose hydrogel	512:549	hydroxypropyl methylcellulose hydrogel	512:549	A hydrogel is typically used as the printer ink for 3D bioprinters, and we incorporated various amounts of hydroxypropyl methylcellulose hydrogel (30%, 40%, and 50% gel) into the printer ink.
30267782	3	38	theme	printer	441:447	arg1	ink					449:451	the printer ink	437:451	the printer ink for 3D bioprinters	437:470	A hydrogel is typically used as the printer ink for 3D bioprinters, and we incorporated various amounts of hydroxypropyl methylcellulose hydrogel (30%, 40%, and 50% gel) into the printer ink.
30267782	3	38	theme	printer	441:447	arg1	amounts					501:507	various amounts	493:507	various amounts of hydroxypropyl methylcellulose hydrogel (30%, 40%, and 50% gel)	493:573	A hydrogel is typically used as the printer ink for 3D bioprinters, and we incorporated various amounts of hydroxypropyl methylcellulose hydrogel (30%, 40%, and 50% gel) into the printer ink.
30267782	3	38	theme	printer	441:447	arg1	hydrogel					407:414	A hydrogel	405:414	A hydrogel	405:414	A hydrogel is typically used as the printer ink for 3D bioprinters, and we incorporated various amounts of hydroxypropyl methylcellulose hydrogel (30%, 40%, and 50% gel) into the printer ink.
30267782	0	39	theme	Resulting	137:145	arg1	Tablets					147:153	the Printed Hydrogel and Resulting Tablets	112:153	Tablets	147:153	Fabrication of Naftopidil-Loaded Tablets Using a Semisolid Extrusion-Type 3D Printer and the Characteristics of the Printed Hydrogel and Resulting Tablets.
30267782	8	40	theme	useful	1286:1291	arg1	information					1293:1303	useful information	1286:1303	useful information for the preparation of tablets using semisolid extrusion-type 3D printers	1286:1377	Our results will provide useful information for the preparation of tablets using semisolid extrusion-type 3D printers.
30267782	6	41	theme	tablets	979:985	arg1	hardness					963:970	hardness	963:970	hardness of the tablets	963:985	Increasing the amount of hydroxypropyl methylcellulose hydrogel in the printer ink led to delayed drug dissolution, decreased weight, and decreased hardness of the tablets.
30267782	3	42	theme	3D	457:458	arg1	bioprinters					460:470	3D bioprinters	457:470	3D bioprinters	457:470	A hydrogel is typically used as the printer ink for 3D bioprinters, and we incorporated various amounts of hydroxypropyl methylcellulose hydrogel (30%, 40%, and 50% gel) into the printer ink.
30267782	0	43	theme	Extrusion-Type	59:72	arg1	Printer					77:83	a Semisolid Extrusion-Type 3D Printer	47:83	a Semisolid Extrusion-Type 3D Printer	47:83	Fabrication of Naftopidil-Loaded Tablets Using a Semisolid Extrusion-Type 3D Printer and the Characteristics of the Printed Hydrogel and Resulting Tablets.
30267782	6	44	theme	drug	913:916	arg1	dissolution					918:928	delayed drug dissolution	905:928	delayed drug dissolution	905:928	Increasing the amount of hydroxypropyl methylcellulose hydrogel in the printer ink led to delayed drug dissolution, decreased weight, and decreased hardness of the tablets.
30267782	7	45	theme	agent	1203:1207	arg1	crospovidone					1209:1220	the disintegrating agent crospovidone	1184:1220	the disintegrating agent crospovidone	1184:1220	Delayed drug dissolution was also observed when the amount of disintegrating agent typically used in powder compression tablets was increased in the ink, and increasing the incorporated amount of the disintegrating agent crospovidone increased the hardness of the tablets.
30267782	1	46	dep	three-dimensional	174:190	arg1	3D					193:194	3D	193:194	3D	193:194	The production of three-dimensional (3D)-printed drugs holds promise for future personalized medicine.
30267782	7	47	theme	drug	996:999	arg1	dissolution					1001:1011	Delayed drug dissolution	988:1011	Delayed drug dissolution	988:1011	Delayed drug dissolution was also observed when the amount of disintegrating agent typically used in powder compression tablets was increased in the ink, and increasing the incorporated amount of the disintegrating agent crospovidone increased the hardness of the tablets.
30267782	2	48	theme	extrusion-type	341:354	arg1	bioprinter					359:368	a semisolid extrusion-type 3D bioprinter	329:368	a semisolid extrusion-type 3D bioprinter applicable for tissue engineering	329:402	Here, we prepared tablets containing naftopidil as a model drug using a semisolid extrusion-type 3D bioprinter applicable for tissue engineering.
30267782	0	49	theme	Semisolid	49:57	arg1	Printer					77:83	a Semisolid Extrusion-Type 3D Printer	47:83	a Semisolid Extrusion-Type 3D Printer	47:83	Fabrication of Naftopidil-Loaded Tablets Using a Semisolid Extrusion-Type 3D Printer and the Characteristics of the Printed Hydrogel and Resulting Tablets.
30267782	6	50	theme	delayed	905:911	arg1	dissolution					918:928	delayed drug dissolution	905:928	delayed drug dissolution	905:928	Increasing the amount of hydroxypropyl methylcellulose hydrogel in the printer ink led to delayed drug dissolution, decreased weight, and decreased hardness of the tablets.
30267782	3	51	theme	50	566:567	arg1	%					568:568	%	568:568	%	568:568	A hydrogel is typically used as the printer ink for 3D bioprinters, and we incorporated various amounts of hydroxypropyl methylcellulose hydrogel (30%, 40%, and 50% gel) into the printer ink.
30267782	7	52	theme	tablets	1252:1258	arg1	hardness					1236:1243	the hardness	1232:1243	the hardness of the tablets	1232:1258	Delayed drug dissolution was also observed when the amount of disintegrating agent typically used in powder compression tablets was increased in the ink, and increasing the incorporated amount of the disintegrating agent crospovidone increased the hardness of the tablets.
30267782	2	53	theme	semisolid	331:339	arg1	bioprinter					359:368	a semisolid extrusion-type 3D bioprinter	329:368	a semisolid extrusion-type 3D bioprinter applicable for tissue engineering	329:402	Here, we prepared tablets containing naftopidil as a model drug using a semisolid extrusion-type 3D bioprinter applicable for tissue engineering.
30267782	3	54	used	used	429:432	arg2	hydrogel					542:549	hydroxypropyl methylcellulose hydrogel	512:549	hydroxypropyl methylcellulose hydrogel	512:549	A hydrogel is typically used as the printer ink for 3D bioprinters, and we incorporated various amounts of hydroxypropyl methylcellulose hydrogel (30%, 40%, and 50% gel) into the printer ink.
30267782	3	54	used	used	429:432	arg2	ink					449:451	the printer ink	437:451	the printer ink for 3D bioprinters	437:470	A hydrogel is typically used as the printer ink for 3D bioprinters, and we incorporated various amounts of hydroxypropyl methylcellulose hydrogel (30%, 40%, and 50% gel) into the printer ink.
30267782	3	54	used	used	429:432	arg2	hydrogel					407:414	A hydrogel	405:414	A hydrogel	405:414	A hydrogel is typically used as the printer ink for 3D bioprinters, and we incorporated various amounts of hydroxypropyl methylcellulose hydrogel (30%, 40%, and 50% gel) into the printer ink.
30267782	3	54	used	used	429:432	arg2	amounts					501:507	various amounts	493:507	various amounts of hydroxypropyl methylcellulose hydrogel (30%, 40%, and 50% gel)	493:573	A hydrogel is typically used as the printer ink for 3D bioprinters, and we incorporated various amounts of hydroxypropyl methylcellulose hydrogel (30%, 40%, and 50% gel) into the printer ink.
30267782	8	55	theme	semisolid	1342:1350	arg1	printers					1370:1377	semisolid extrusion-type 3D printers	1342:1377	semisolid extrusion-type 3D printers	1342:1377	Our results will provide useful information for the preparation of tablets using semisolid extrusion-type 3D printers.
30267782	5	56	theme	printer	697:703	arg1	ink					705:707	the printer ink	693:707	the printer ink	693:707	The rheological properties of the printer ink changed as its composition was changed, and tablets were prepared successfully from several formulations.
30267782	3	57	theme	printer	584:590	arg1	ink					592:594	the printer ink	580:594	the printer ink	580:594	A hydrogel is typically used as the printer ink for 3D bioprinters, and we incorporated various amounts of hydroxypropyl methylcellulose hydrogel (30%, 40%, and 50% gel) into the printer ink.
30267782	3	58	theme	methylcellulose	526:540	arg1	hydrogel					542:549	hydroxypropyl methylcellulose hydrogel	512:549	hydroxypropyl methylcellulose hydrogel	512:549	A hydrogel is typically used as the printer ink for 3D bioprinters, and we incorporated various amounts of hydroxypropyl methylcellulose hydrogel (30%, 40%, and 50% gel) into the printer ink.
30267782	1	59	theme	future	229:234	arg1	medicine					249:256	future personalized medicine	229:256	future personalized medicine	229:256	The production of three-dimensional (3D)-printed drugs holds promise for future personalized medicine.
30267782	7	60	theme	disintegrating	1188:1201	arg1	crospovidone					1209:1220	the disintegrating agent crospovidone	1184:1220	the disintegrating agent crospovidone	1184:1220	Delayed drug dissolution was also observed when the amount of disintegrating agent typically used in powder compression tablets was increased in the ink, and increasing the incorporated amount of the disintegrating agent crospovidone increased the hardness of the tablets.
30267782	3	61	theme	hydroxypropyl	512:524	arg1	hydrogel					542:549	hydroxypropyl methylcellulose hydrogel	512:549	hydroxypropyl methylcellulose hydrogel	512:549	A hydrogel is typically used as the printer ink for 3D bioprinters, and we incorporated various amounts of hydroxypropyl methylcellulose hydrogel (30%, 40%, and 50% gel) into the printer ink.
30267782	1	62	theme	personalized	236:247	arg1	medicine					249:256	future personalized medicine	229:256	future personalized medicine	229:256	The production of three-dimensional (3D)-printed drugs holds promise for future personalized medicine.
30267782	0	63	theme	3D	74:75	arg1	Printer					77:83	a Semisolid Extrusion-Type 3D Printer	47:83	a Semisolid Extrusion-Type 3D Printer	47:83	Fabrication of Naftopidil-Loaded Tablets Using a Semisolid Extrusion-Type 3D Printer and the Characteristics of the Printed Hydrogel and Resulting Tablets.
30267782	6	64	from	amount	830:835	arg1	ink					894:896	the printer ink	882:896	the printer ink	882:896	Increasing the amount of hydroxypropyl methylcellulose hydrogel in the printer ink led to delayed drug dissolution, decreased weight, and decreased hardness of the tablets.
30267782	2	65	theme	applicable	370:379	arg1	bioprinter					359:368	a semisolid extrusion-type 3D bioprinter	329:368	a semisolid extrusion-type 3D bioprinter applicable for tissue engineering	329:402	Here, we prepared tablets containing naftopidil as a model drug using a semisolid extrusion-type 3D bioprinter applicable for tissue engineering.
31760033	0	0	theme	adsorbent	100:108	arg1	Evaluation					86:95	Evaluation	86:95	Magnetite nanoparticle embedded Pectin-graft-poly(N-hydroxyethylacrylamide) hydrogel: Evaluation as adsorbent for dyes and heavy metal ions from waste water.	0:156	Magnetite nanoparticle embedded Pectin-graft-poly(N-hydroxyethylacrylamide) hydrogel: Evaluation as adsorbent for dyes and heavy metal ions from waste water.
31760033	8	1	theme	first	1333:1337	arg1	adsorption					1297:1306	The adsorption	1293:1306	The adsorption	1293:1306	The adsorption was found to be a pseudo first order process for R6G and pseudo second order process for Cu(II) and Hg(II) ions.
31760033	8	1	theme	first	1333:1337	arg1	process					1345:1351	a pseudo first order process	1324:1351	a pseudo first order process for R6G	1324:1359	The adsorption was found to be a pseudo first order process for R6G and pseudo second order process for Cu(II) and Hg(II) ions.
31760033	5	2	theme	metal	897:901	arg1	ions					903:906	metal ions	897:906	metal ions	897:906	The Pec-g-PHEAA and Pec-g-PHEAA/Fe3O4 systems were evaluated for removal of dye and metal ions from aqueous solution using Rhodamine 6G (R6G), a cationic dye; Cu(II) and Hg(II) ions.
31760033	3	3	theme	BET	702:704	arg1	techniques					714:723	FTIR, TGA, XRD, BET and SEM techniques	686:723	FTIR, TGA, XRD, BET and SEM techniques	686:723	The synthesized gel, pectin-graft-poly(N-hydroxyethylacrylamide) (Pec-g-PHEAA); and the magnetite containing composite (Pec-g-PHEAA/Fe3O4) were characterized by FTIR, TGA, XRD, BET and SEM techniques.
31760033	8	4	theme	order	1339:1343	arg1	adsorption					1297:1306	The adsorption	1293:1306	The adsorption	1293:1306	The adsorption was found to be a pseudo first order process for R6G and pseudo second order process for Cu(II) and Hg(II) ions.
31760033	8	4	theme	order	1339:1343	arg1	process					1345:1351	a pseudo first order process	1324:1351	a pseudo first order process for R6G	1324:1359	The adsorption was found to be a pseudo first order process for R6G and pseudo second order process for Cu(II) and Hg(II) ions.
31760033	3	5	theme	FTIR	686:689	arg1	techniques					714:723	FTIR, TGA, XRD, BET and SEM techniques	686:723	FTIR, TGA, XRD, BET and SEM techniques	686:723	The synthesized gel, pectin-graft-poly(N-hydroxyethylacrylamide) (Pec-g-PHEAA); and the magnetite containing composite (Pec-g-PHEAA/Fe3O4) were characterized by FTIR, TGA, XRD, BET and SEM techniques.
31760033	8	6	theme	pseudo	1326:1331	arg1	adsorption					1297:1306	The adsorption	1293:1306	The adsorption	1293:1306	The adsorption was found to be a pseudo first order process for R6G and pseudo second order process for Cu(II) and Hg(II) ions.
31760033	8	6	theme	pseudo	1326:1331	arg1	process					1345:1351	a pseudo first order process	1324:1351	a pseudo first order process for R6G	1324:1359	The adsorption was found to be a pseudo first order process for R6G and pseudo second order process for Cu(II) and Hg(II) ions.
31760033	1	7	theme	pectin	160:165	arg1	gel					179:181	A pectin (Pec) based gel	158:181	A pectin (Pec) based gel	158:181	A pectin (Pec) based gel has been made by grafting N-hydroxyethylacrylamide (HEAA) on pectin using potassium peroxodisulphate as initiator and N,N-methylenebisacrylamide as crosslinker under microwave irradiation.
31760033	6	8	theme	adsorption	1031:1040	arg1	capacity					1042:1049	significant adsorption capacity	1019:1049	significant adsorption capacity towards these species	1019:1071	Both adsorbents showed significant adsorption capacity towards these species, with greater adsorption capacity in case of Pec-g-PHEAA/Fe3O4.
31760033	4	9	theme	ferromagnetic	770:782	arg1	nature					784:789	ferromagnetic nature	770:789	ferromagnetic nature of the nanocomposite	770:810	The magnetic property measurement indicated ferromagnetic nature of the nanocomposite.
31760033	9	10	theme	adsorption	1493:1502	arg1	process					1504:1510	the adsorption process	1489:1510	the adsorption process	1489:1510	The positive values of ∆H0 and the negative values of ∆G0 indicated the adsorption process to be endothermic and spontaneous.
31760033	9	10	theme	adsorption	1493:1502	arg1	endothermic					1518:1528	endothermic	1518:1528	endothermic	1518:1528	The positive values of ∆H0 and the negative values of ∆G0 indicated the adsorption process to be endothermic and spontaneous.
31760033	3	11	theme	synthesized	529:539	arg1	Pec-g-PHEAA					591:601	The synthesized gel, pectin-graft-poly(N-hydroxyethylacrylamide) (Pec-g-PHEAA);	525:603	Pec-g-PHEAA	591:601	The synthesized gel, pectin-graft-poly(N-hydroxyethylacrylamide) (Pec-g-PHEAA); and the magnetite containing composite (Pec-g-PHEAA/Fe3O4) were characterized by FTIR, TGA, XRD, BET and SEM techniques.
31760033	6	12	theme	greater	1079:1085	arg1	capacity					1098:1105	greater adsorption capacity	1079:1105	greater adsorption capacity in case of Pec-g-PHEAA/Fe3O4	1079:1134	Both adsorbents showed significant adsorption capacity towards these species, with greater adsorption capacity in case of Pec-g-PHEAA/Fe3O4.
31760033	2	13	theme	ammonia	507:513	arg1	solution					515:522	ammonia solution	507:522	ammonia solution	507:522	The magnetite (Fe3O4) nanoparticles were incorporated within this gel via in situ diffusion of Fe2+ and Fe3+ followed by reaction with ammonia solution.
31760033	1	14	theme	microwave	349:357	arg1	irradiation					359:369	microwave irradiation	349:369	microwave irradiation	349:369	A pectin (Pec) based gel has been made by grafting N-hydroxyethylacrylamide (HEAA) on pectin using potassium peroxodisulphate as initiator and N,N-methylenebisacrylamide as crosslinker under microwave irradiation.
31760033	4	15	theme	magnetic	730:737	arg1	measurement					748:758	The magnetic property measurement	726:758	The magnetic property measurement	726:758	The magnetic property measurement indicated ferromagnetic nature of the nanocomposite.
31760033	7	16	theme	Freundlich	1196:1205	arg1	models					1216:1221	both Langmuir and Freundlich isotherm models	1178:1221	models	1216:1221	Adsorption process is observed to follow both Langmuir and Freundlich isotherm models for R6G dye and Freundlich isotherm model for Cu(II) and Hg(II) ions.
31760033	3	17	theme	gel	541:543	arg1	Pec-g-PHEAA					591:601	The synthesized gel, pectin-graft-poly(N-hydroxyethylacrylamide) (Pec-g-PHEAA);	525:603	Pec-g-PHEAA	591:601	The synthesized gel, pectin-graft-poly(N-hydroxyethylacrylamide) (Pec-g-PHEAA); and the magnetite containing composite (Pec-g-PHEAA/Fe3O4) were characterized by FTIR, TGA, XRD, BET and SEM techniques.
31760033	0	18	from	water	151:155	arg1	dyes					114:117	dyes	114:117	dyes	114:117	Magnetite nanoparticle embedded Pectin-graft-poly(N-hydroxyethylacrylamide) hydrogel: Evaluation as adsorbent for dyes and heavy metal ions from waste water.
31760033	0	18	from	water	151:155	arg1	ions					135:138	heavy metal ions	123:138	heavy metal ions	123:138	Magnetite nanoparticle embedded Pectin-graft-poly(N-hydroxyethylacrylamide) hydrogel: Evaluation as adsorbent for dyes and heavy metal ions from waste water.
31760033	0	19	theme	heavy	123:127	arg1	ions					135:138	heavy metal ions	123:138	heavy metal ions	123:138	Magnetite nanoparticle embedded Pectin-graft-poly(N-hydroxyethylacrylamide) hydrogel: Evaluation as adsorbent for dyes and heavy metal ions from waste water.
31760033	7	20	theme	Cu	1269:1270	arg1	ions					1287:1290	Cu(II) and Hg(II) ions	1269:1290	Cu(II) and Hg(II) ions	1269:1290	Adsorption process is observed to follow both Langmuir and Freundlich isotherm models for R6G dye and Freundlich isotherm model for Cu(II) and Hg(II) ions.
31760033	8	21	theme	Hg	1408:1409	arg1	ions					1415:1418	Cu(II) and Hg(II) ions	1397:1418	Cu(II) and Hg(II) ions	1397:1418	The adsorption was found to be a pseudo first order process for R6G and pseudo second order process for Cu(II) and Hg(II) ions.
31760033	0	22	theme	N-hydroxyethylacrylamide	50:73	arg1	hydrogel					76:83	Magnetite nanoparticle embedded Pectin-graft-poly(N-hydroxyethylacrylamide) hydrogel	0:83	Magnetite nanoparticle embedded Pectin-graft-poly(N-hydroxyethylacrylamide) hydrogel: Evaluation as adsorbent for dyes and heavy metal ions from waste water.	0:156	Magnetite nanoparticle embedded Pectin-graft-poly(N-hydroxyethylacrylamide) hydrogel: Evaluation as adsorbent for dyes and heavy metal ions from waste water.
31760033	5	23	from	solution	921:928	arg1	removal					878:884	removal	878:884	removal of dye and metal ions from aqueous solution	878:928	The Pec-g-PHEAA and Pec-g-PHEAA/Fe3O4 systems were evaluated for removal of dye and metal ions from aqueous solution using Rhodamine 6G (R6G), a cationic dye; Cu(II) and Hg(II) ions.
31760033	5	24	theme	cationic	958:965	arg1	dye					967:969	a cationic dye	956:969	a cationic dye	956:969	The Pec-g-PHEAA and Pec-g-PHEAA/Fe3O4 systems were evaluated for removal of dye and metal ions from aqueous solution using Rhodamine 6G (R6G), a cationic dye; Cu(II) and Hg(II) ions.
31760033	5	24	theme	cationic	958:965	arg1	6G					946:947	Rhodamine 6G	936:947	Rhodamine 6G (R6G)	936:953	The Pec-g-PHEAA and Pec-g-PHEAA/Fe3O4 systems were evaluated for removal of dye and metal ions from aqueous solution using Rhodamine 6G (R6G), a cationic dye; Cu(II) and Hg(II) ions.
31760033	6	25	theme	significant	1019:1029	arg1	capacity					1042:1049	significant adsorption capacity	1019:1049	significant adsorption capacity towards these species	1019:1071	Both adsorbents showed significant adsorption capacity towards these species, with greater adsorption capacity in case of Pec-g-PHEAA/Fe3O4.
31760033	1	26	theme	Pec	168:170	arg1	gel					179:181	A pectin (Pec) based gel	158:181	A pectin (Pec) based gel	158:181	A pectin (Pec) based gel has been made by grafting N-hydroxyethylacrylamide (HEAA) on pectin using potassium peroxodisulphate as initiator and N,N-methylenebisacrylamide as crosslinker under microwave irradiation.
31760033	2	27	theme	in	446:447	arg1	diffusion					454:462	in situ diffusion	446:462	in situ diffusion of Fe2+	446:470	The magnetite (Fe3O4) nanoparticles were incorporated within this gel via in situ diffusion of Fe2+ and Fe3+ followed by reaction with ammonia solution.
31760033	2	28	dep	in	446:447	arg1	situ					449:452	situ	449:452	situ	449:452	The magnetite (Fe3O4) nanoparticles were incorporated within this gel via in situ diffusion of Fe2+ and Fe3+ followed by reaction with ammonia solution.
31760033	2	29	with	reaction	493:500	arg1	solution					515:522	ammonia solution	507:522	ammonia solution	507:522	The magnetite (Fe3O4) nanoparticles were incorporated within this gel via in situ diffusion of Fe2+ and Fe3+ followed by reaction with ammonia solution.
31760033	5	30	theme	Rhodamine	936:944	arg1	dye					967:969	a cationic dye	956:969	a cationic dye	956:969	The Pec-g-PHEAA and Pec-g-PHEAA/Fe3O4 systems were evaluated for removal of dye and metal ions from aqueous solution using Rhodamine 6G (R6G), a cationic dye; Cu(II) and Hg(II) ions.
31760033	5	30	theme	Rhodamine	936:944	arg1	R6G					950:952	R6G	950:952	R6G	950:952	The Pec-g-PHEAA and Pec-g-PHEAA/Fe3O4 systems were evaluated for removal of dye and metal ions from aqueous solution using Rhodamine 6G (R6G), a cationic dye; Cu(II) and Hg(II) ions.
31760033	5	30	theme	Rhodamine	936:944	arg1	6G					946:947	Rhodamine 6G	936:947	Rhodamine 6G (R6G)	936:953	The Pec-g-PHEAA and Pec-g-PHEAA/Fe3O4 systems were evaluated for removal of dye and metal ions from aqueous solution using Rhodamine 6G (R6G), a cationic dye; Cu(II) and Hg(II) ions.
31760033	3	31	dep	Pec-g-PHEAA	591:601	arg1	pectin-graft-poly					546:562	The synthesized gel, pectin-graft-poly(N-hydroxyethylacrylamide) (Pec-g-PHEAA);	525:603	pectin-graft-poly	546:562	The synthesized gel, pectin-graft-poly(N-hydroxyethylacrylamide) (Pec-g-PHEAA); and the magnetite containing composite (Pec-g-PHEAA/Fe3O4) were characterized by FTIR, TGA, XRD, BET and SEM techniques.
31760033	0	32	theme	Magnetite	0:8	arg1	hydrogel					76:83	Magnetite nanoparticle embedded Pectin-graft-poly(N-hydroxyethylacrylamide) hydrogel	0:83	Magnetite nanoparticle embedded Pectin-graft-poly(N-hydroxyethylacrylamide) hydrogel: Evaluation as adsorbent for dyes and heavy metal ions from waste water.	0:156	Magnetite nanoparticle embedded Pectin-graft-poly(N-hydroxyethylacrylamide) hydrogel: Evaluation as adsorbent for dyes and heavy metal ions from waste water.
31760033	3	33	theme	N-hydroxyethylacrylamide	564:587	arg1	Pec-g-PHEAA					591:601	The synthesized gel, pectin-graft-poly(N-hydroxyethylacrylamide) (Pec-g-PHEAA);	525:603	Pec-g-PHEAA	591:601	The synthesized gel, pectin-graft-poly(N-hydroxyethylacrylamide) (Pec-g-PHEAA); and the magnetite containing composite (Pec-g-PHEAA/Fe3O4) were characterized by FTIR, TGA, XRD, BET and SEM techniques.
31760033	4	34	theme	property	739:746	arg1	measurement					748:758	The magnetic property measurement	726:758	The magnetic property measurement	726:758	The magnetic property measurement indicated ferromagnetic nature of the nanocomposite.
31760033	2	35	theme	magnetite	376:384	arg1	nanoparticles					394:406	The magnetite (Fe3O4) nanoparticles	372:406	The magnetite (Fe3O4) nanoparticles	372:406	The magnetite (Fe3O4) nanoparticles were incorporated within this gel via in situ diffusion of Fe2+ and Fe3+ followed by reaction with ammonia solution.
31760033	0	36	theme	Pectin-graft-poly	32:48	arg1	hydrogel					76:83	Magnetite nanoparticle embedded Pectin-graft-poly(N-hydroxyethylacrylamide) hydrogel	0:83	Magnetite nanoparticle embedded Pectin-graft-poly(N-hydroxyethylacrylamide) hydrogel: Evaluation as adsorbent for dyes and heavy metal ions from waste water.	0:156	Magnetite nanoparticle embedded Pectin-graft-poly(N-hydroxyethylacrylamide) hydrogel: Evaluation as adsorbent for dyes and heavy metal ions from waste water.
31760033	1	37	theme	potassium	257:265	arg1	peroxodisulphate					267:282	potassium peroxodisulphate	257:282	potassium peroxodisulphate	257:282	A pectin (Pec) based gel has been made by grafting N-hydroxyethylacrylamide (HEAA) on pectin using potassium peroxodisulphate as initiator and N,N-methylenebisacrylamide as crosslinker under microwave irradiation.
31760033	6	38	theme	adsorption	1087:1096	arg1	capacity					1098:1105	greater adsorption capacity	1079:1105	greater adsorption capacity in case of Pec-g-PHEAA/Fe3O4	1079:1134	Both adsorbents showed significant adsorption capacity towards these species, with greater adsorption capacity in case of Pec-g-PHEAA/Fe3O4.
31760033	0	39	theme	embedded	23:30	arg1	hydrogel					76:83	Magnetite nanoparticle embedded Pectin-graft-poly(N-hydroxyethylacrylamide) hydrogel	0:83	Magnetite nanoparticle embedded Pectin-graft-poly(N-hydroxyethylacrylamide) hydrogel: Evaluation as adsorbent for dyes and heavy metal ions from waste water.	0:156	Magnetite nanoparticle embedded Pectin-graft-poly(N-hydroxyethylacrylamide) hydrogel: Evaluation as adsorbent for dyes and heavy metal ions from waste water.
31760033	3	40	theme	SEM	710:712	arg1	techniques					714:723	FTIR, TGA, XRD, BET and SEM techniques	686:723	FTIR, TGA, XRD, BET and SEM techniques	686:723	The synthesized gel, pectin-graft-poly(N-hydroxyethylacrylamide) (Pec-g-PHEAA); and the magnetite containing composite (Pec-g-PHEAA/Fe3O4) were characterized by FTIR, TGA, XRD, BET and SEM techniques.
31760033	0	41	theme	metal	129:133	arg1	ions					135:138	heavy metal ions	123:138	heavy metal ions	123:138	Magnetite nanoparticle embedded Pectin-graft-poly(N-hydroxyethylacrylamide) hydrogel: Evaluation as adsorbent for dyes and heavy metal ions from waste water.
31760033	5	42	theme	ions	903:906	arg1	removal					878:884	removal	878:884	removal of dye and metal ions from aqueous solution	878:928	The Pec-g-PHEAA and Pec-g-PHEAA/Fe3O4 systems were evaluated for removal of dye and metal ions from aqueous solution using Rhodamine 6G (R6G), a cationic dye; Cu(II) and Hg(II) ions.
31760033	5	43	theme	Pec-g-PHEAA/Fe3O4	833:849	arg1	systems					851:857	The Pec-g-PHEAA and Pec-g-PHEAA/Fe3O4 systems	813:857	The Pec-g-PHEAA and Pec-g-PHEAA/Fe3O4 systems	813:857	The Pec-g-PHEAA and Pec-g-PHEAA/Fe3O4 systems were evaluated for removal of dye and metal ions from aqueous solution using Rhodamine 6G (R6G), a cationic dye; Cu(II) and Hg(II) ions.
31760033	2	44	theme	Fe2+	467:470	arg1	diffusion					454:462	in situ diffusion	446:462	in situ diffusion of Fe2+	446:470	The magnetite (Fe3O4) nanoparticles were incorporated within this gel via in situ diffusion of Fe2+ and Fe3+ followed by reaction with ammonia solution.
31760033	0	45	theme	waste	145:149	arg1	water					151:155	waste water	145:155	waste water	145:155	Magnetite nanoparticle embedded Pectin-graft-poly(N-hydroxyethylacrylamide) hydrogel: Evaluation as adsorbent for dyes and heavy metal ions from waste water.
31760033	7	46	theme	isotherm	1207:1214	arg1	models					1216:1221	both Langmuir and Freundlich isotherm models	1178:1221	models	1216:1221	Adsorption process is observed to follow both Langmuir and Freundlich isotherm models for R6G dye and Freundlich isotherm model for Cu(II) and Hg(II) ions.
31760033	0	47	dep	hydrogel	76:83	arg1	Evaluation					86:95	Evaluation	86:95	Magnetite nanoparticle embedded Pectin-graft-poly(N-hydroxyethylacrylamide) hydrogel: Evaluation as adsorbent for dyes and heavy metal ions from waste water.	0:156	Magnetite nanoparticle embedded Pectin-graft-poly(N-hydroxyethylacrylamide) hydrogel: Evaluation as adsorbent for dyes and heavy metal ions from waste water.
31760033	1	48	theme	based	173:177	arg1	gel					179:181	A pectin (Pec) based gel	158:181	A pectin (Pec) based gel	158:181	A pectin (Pec) based gel has been made by grafting N-hydroxyethylacrylamide (HEAA) on pectin using potassium peroxodisulphate as initiator and N,N-methylenebisacrylamide as crosslinker under microwave irradiation.
31760033	7	49	theme	Freundlich	1239:1248	arg1	model					1259:1263	R6G dye and Freundlich isotherm model	1227:1263	model	1259:1263	Adsorption process is observed to follow both Langmuir and Freundlich isotherm models for R6G dye and Freundlich isotherm model for Cu(II) and Hg(II) ions.
31760033	3	50	theme	containing	623:632	arg1	Pec-g-PHEAA/Fe3O4					645:661	Pec-g-PHEAA/Fe3O4	645:661	Pec-g-PHEAA/Fe3O4	645:661	The synthesized gel, pectin-graft-poly(N-hydroxyethylacrylamide) (Pec-g-PHEAA); and the magnetite containing composite (Pec-g-PHEAA/Fe3O4) were characterized by FTIR, TGA, XRD, BET and SEM techniques.
31760033	3	50	theme	containing	623:632	arg1	composite					634:642	the magnetite containing composite	609:642	the magnetite containing composite (Pec-g-PHEAA/Fe3O4)	609:662	The synthesized gel, pectin-graft-poly(N-hydroxyethylacrylamide) (Pec-g-PHEAA); and the magnetite containing composite (Pec-g-PHEAA/Fe3O4) were characterized by FTIR, TGA, XRD, BET and SEM techniques.
31760033	4	51	theme	nanocomposite	798:810	arg1	nature					784:789	ferromagnetic nature	770:789	ferromagnetic nature of the nanocomposite	770:810	The magnetic property measurement indicated ferromagnetic nature of the nanocomposite.
31760033	7	52	theme	isotherm	1250:1257	arg1	model					1259:1263	R6G dye and Freundlich isotherm model	1227:1263	model	1259:1263	Adsorption process is observed to follow both Langmuir and Freundlich isotherm models for R6G dye and Freundlich isotherm model for Cu(II) and Hg(II) ions.
31760033	5	53	dep	evaluated	864:872	arg1	ions					990:993	Cu(II) and Hg(II) ions	972:993	Cu(II) and Hg(II) ions	972:993	The Pec-g-PHEAA and Pec-g-PHEAA/Fe3O4 systems were evaluated for removal of dye and metal ions from aqueous solution using Rhodamine 6G (R6G), a cationic dye; Cu(II) and Hg(II) ions.
31760033	8	54	theme	Cu	1397:1398	arg1	ions					1415:1418	Cu(II) and Hg(II) ions	1397:1418	Cu(II) and Hg(II) ions	1397:1418	The adsorption was found to be a pseudo first order process for R6G and pseudo second order process for Cu(II) and Hg(II) ions.
31760033	8	55	theme	pseudo	1365:1370	arg1	process					1385:1391	pseudo second order process	1365:1391	pseudo second order process for Cu(II) and Hg(II) ions	1365:1418	The adsorption was found to be a pseudo first order process for R6G and pseudo second order process for Cu(II) and Hg(II) ions.
31760033	5	56	theme	Hg	983:984	arg1	ions					990:993	Cu(II) and Hg(II) ions	972:993	Cu(II) and Hg(II) ions	972:993	The Pec-g-PHEAA and Pec-g-PHEAA/Fe3O4 systems were evaluated for removal of dye and metal ions from aqueous solution using Rhodamine 6G (R6G), a cationic dye; Cu(II) and Hg(II) ions.
31760033	5	57	theme	aqueous	913:919	arg1	solution					921:928	aqueous solution	913:928	aqueous solution	913:928	The Pec-g-PHEAA and Pec-g-PHEAA/Fe3O4 systems were evaluated for removal of dye and metal ions from aqueous solution using Rhodamine 6G (R6G), a cationic dye; Cu(II) and Hg(II) ions.
31760033	7	58	theme	Hg	1280:1281	arg1	ions					1287:1290	Cu(II) and Hg(II) ions	1269:1290	Cu(II) and Hg(II) ions	1269:1290	Adsorption process is observed to follow both Langmuir and Freundlich isotherm models for R6G dye and Freundlich isotherm model for Cu(II) and Hg(II) ions.
31760033	9	59	theme	negative	1456:1463	arg1	values					1465:1470	the negative values	1452:1470	the negative values of ∆G0	1452:1477	The positive values of ∆H0 and the negative values of ∆G0 indicated the adsorption process to be endothermic and spontaneous.
31760033	9	60	theme	∆H0	1444:1446	arg1	values					1465:1470	the negative values	1452:1470	the negative values of ∆G0	1452:1477	The positive values of ∆H0 and the negative values of ∆G0 indicated the adsorption process to be endothermic and spontaneous.
31760033	9	60	theme	∆H0	1444:1446	arg1	values					1434:1439	The positive values	1421:1439	The positive values of ∆H0	1421:1446	The positive values of ∆H0 and the negative values of ∆G0 indicated the adsorption process to be endothermic and spontaneous.
31760033	9	61	theme	positive	1425:1432	arg1	values					1434:1439	The positive values	1421:1439	The positive values of ∆H0	1421:1446	The positive values of ∆H0 and the negative values of ∆G0 indicated the adsorption process to be endothermic and spontaneous.
31760033	8	62	theme	order	1379:1383	arg1	process					1385:1391	pseudo second order process	1365:1391	pseudo second order process for Cu(II) and Hg(II) ions	1365:1418	The adsorption was found to be a pseudo first order process for R6G and pseudo second order process for Cu(II) and Hg(II) ions.
31760033	3	63	theme	magnetite	613:621	arg1	Pec-g-PHEAA/Fe3O4					645:661	Pec-g-PHEAA/Fe3O4	645:661	Pec-g-PHEAA/Fe3O4	645:661	The synthesized gel, pectin-graft-poly(N-hydroxyethylacrylamide) (Pec-g-PHEAA); and the magnetite containing composite (Pec-g-PHEAA/Fe3O4) were characterized by FTIR, TGA, XRD, BET and SEM techniques.
31760033	3	63	theme	magnetite	613:621	arg1	composite					634:642	the magnetite containing composite	609:642	the magnetite containing composite (Pec-g-PHEAA/Fe3O4)	609:662	The synthesized gel, pectin-graft-poly(N-hydroxyethylacrylamide) (Pec-g-PHEAA); and the magnetite containing composite (Pec-g-PHEAA/Fe3O4) were characterized by FTIR, TGA, XRD, BET and SEM techniques.
31760033	7	64	theme	R6G	1227:1229	arg1	dye					1231:1233	R6G dye and Freundlich isotherm model	1227:1263	dye	1231:1233	Adsorption process is observed to follow both Langmuir and Freundlich isotherm models for R6G dye and Freundlich isotherm model for Cu(II) and Hg(II) ions.
31760033	8	65	theme	second	1372:1377	arg1	process					1385:1391	pseudo second order process	1365:1391	pseudo second order process for Cu(II) and Hg(II) ions	1365:1418	The adsorption was found to be a pseudo first order process for R6G and pseudo second order process for Cu(II) and Hg(II) ions.
31760033	5	66	theme	Cu	972:973	arg1	ions					990:993	Cu(II) and Hg(II) ions	972:993	Cu(II) and Hg(II) ions	972:993	The Pec-g-PHEAA and Pec-g-PHEAA/Fe3O4 systems were evaluated for removal of dye and metal ions from aqueous solution using Rhodamine 6G (R6G), a cationic dye; Cu(II) and Hg(II) ions.
31760033	5	67	theme	dye	889:891	arg1	removal					878:884	removal	878:884	removal of dye and metal ions from aqueous solution	878:928	The Pec-g-PHEAA and Pec-g-PHEAA/Fe3O4 systems were evaluated for removal of dye and metal ions from aqueous solution using Rhodamine 6G (R6G), a cationic dye; Cu(II) and Hg(II) ions.
31760033	2	68	theme	Fe3O4	387:391	arg1	nanoparticles					394:406	The magnetite (Fe3O4) nanoparticles	372:406	The magnetite (Fe3O4) nanoparticles	372:406	The magnetite (Fe3O4) nanoparticles were incorporated within this gel via in situ diffusion of Fe2+ and Fe3+ followed by reaction with ammonia solution.
31760033	7	69	theme	Adsorption	1137:1146	arg1	process					1148:1154	Adsorption process	1137:1154	Adsorption process	1137:1154	Adsorption process is observed to follow both Langmuir and Freundlich isotherm models for R6G dye and Freundlich isotherm model for Cu(II) and Hg(II) ions.
31760033	3	70	theme	TGA	692:694	arg1	techniques					714:723	FTIR, TGA, XRD, BET and SEM techniques	686:723	FTIR, TGA, XRD, BET and SEM techniques	686:723	The synthesized gel, pectin-graft-poly(N-hydroxyethylacrylamide) (Pec-g-PHEAA); and the magnetite containing composite (Pec-g-PHEAA/Fe3O4) were characterized by FTIR, TGA, XRD, BET and SEM techniques.
31760033	5	71	theme	Pec-g-PHEAA	817:827	arg1	systems					851:857	The Pec-g-PHEAA and Pec-g-PHEAA/Fe3O4 systems	813:857	The Pec-g-PHEAA and Pec-g-PHEAA/Fe3O4 systems	813:857	The Pec-g-PHEAA and Pec-g-PHEAA/Fe3O4 systems were evaluated for removal of dye and metal ions from aqueous solution using Rhodamine 6G (R6G), a cationic dye; Cu(II) and Hg(II) ions.
31760033	0	72	theme	nanoparticle	10:21	arg1	hydrogel					76:83	Magnetite nanoparticle embedded Pectin-graft-poly(N-hydroxyethylacrylamide) hydrogel	0:83	Magnetite nanoparticle embedded Pectin-graft-poly(N-hydroxyethylacrylamide) hydrogel: Evaluation as adsorbent for dyes and heavy metal ions from waste water.	0:156	Magnetite nanoparticle embedded Pectin-graft-poly(N-hydroxyethylacrylamide) hydrogel: Evaluation as adsorbent for dyes and heavy metal ions from waste water.
31760033	9	73	theme	∆G0	1475:1477	arg1	values					1465:1470	the negative values	1452:1470	the negative values of ∆G0	1452:1477	The positive values of ∆H0 and the negative values of ∆G0 indicated the adsorption process to be endothermic and spontaneous.
31760033	9	73	theme	∆G0	1475:1477	arg1	values					1434:1439	The positive values	1421:1439	The positive values of ∆H0	1421:1446	The positive values of ∆H0 and the negative values of ∆G0 indicated the adsorption process to be endothermic and spontaneous.
31590881	7	0	dep	bacteria	1156:1163	arg1	coli					1150:1153	E. coli	1147:1153	E. coli	1147:1153	The antibacterial activity of Ch-ge-Q film against both gram positive (B. substilis) and gram negative (E. coli) bacteria suggested the Q loaded Ch-ge films as more feasible antibacterial candidate especially against the strain E. coli.
31590881	3	1	theme	water	527:531	arg1	properties					544:553	water solubility properties	527:553	water solubility properties	527:553	The films were also investigated for their swelling, water-vapor permeability (WVP), water solubility properties.
31590881	3	1	theme	water	527:531	arg1	swelling					485:492	their swelling	479:492	their swelling	479:492	The films were also investigated for their swelling, water-vapor permeability (WVP), water solubility properties.
31590881	6	2	from	decrease	949:956	arg1	break					975:979	break	975:979	break	975:979	The reduction of water-vapor permeability and increase in tensile strength while a decrease in elongation at break has been observed in the Ch-ge-Q film compared to Ch-ge film.
31590881	6	2	from	decrease	949:956	arg1	elongation					961:970	elongation	961:970	elongation at break	961:979	The reduction of water-vapor permeability and increase in tensile strength while a decrease in elongation at break has been observed in the Ch-ge-Q film compared to Ch-ge film.
31590881	7	3	theme	negative	1137:1144	arg1	bacteria					1156:1163	both gram positive (B. substilis) and gram negative (E. coli) bacteria	1094:1163	both gram positive (B. substilis) and gram negative (E. coli) bacteria	1094:1163	The antibacterial activity of Ch-ge-Q film against both gram positive (B. substilis) and gram negative (E. coli) bacteria suggested the Q loaded Ch-ge films as more feasible antibacterial candidate especially against the strain E. coli.
31590881	2	4	theme	UV	408:409	arg1	techniques					430:439	UV, FTIR, XRD and SEM techniques	408:439	UV, FTIR, XRD and SEM techniques	408:439	Physicochemical characteristics and mechanical properties of the resulting chitosan-gelatin containing Quercetin-starch films (Ch-ge-Q) were studied using UV, FTIR, XRD and SEM techniques.
31590881	8	5	theme	Ch-ge-Q	1312:1318	arg1	film					1320:1323	the Ch-ge-Q film	1308:1323	the Ch-ge-Q film	1308:1323	The antioxidant activity of the Ch-ge-Q film was evaluated using the DPPH and ABTS as standards and corresponded to 81.45% of DPPH and 72.2% of ABTS scavenging activities.
31590881	9	6	theme	film	1581:1584	arg1	application					1600:1610	Ch-ge film promising its application	1575:1610	Ch-ge film promising its application in food packaging	1575:1628	It was observed that the film containing Quercetin-starch presented superior antioxidant activity results in comparison to Ch-ge film promising its application in food packaging.
31590881	4	7	theme	Q	799:799	arg1	addition					787:794	the addition	783:794	the addition of Q	783:799	The FTIR spectra confirmed the chemical interactions between the chitosan-gelatin and Q. Surface morphology of prepared film was analyzed by the SEM imaging while XRD spectra suggest the expanded crystallinity of the film with the addition of Q.
31590881	1	8	theme	Ecofriendly	116:126	arg1	films					167:171	Ecofriendly chitosan-gelatin (Ch-ge) bio-composite films	116:171	Ecofriendly chitosan-gelatin (Ch-ge) bio-composite films containing Quercetin-starch (Q)	116:203	Ecofriendly chitosan-gelatin (Ch-ge) bio-composite films containing Quercetin-starch (Q) were synthesized using solution casting method.
31590881	4	9	theme	Q.	642:643	arg1	morphology					653:662	Q. Surface morphology	642:662	Q. Surface morphology of prepared film	642:679	The FTIR spectra confirmed the chemical interactions between the chitosan-gelatin and Q. Surface morphology of prepared film was analyzed by the SEM imaging while XRD spectra suggest the expanded crystallinity of the film with the addition of Q.
31590881	0	10	theme	food	100:103	arg1	packaging					105:113	food packaging	100:113	food packaging	100:113	Preparation, physicochemical and biological evaluation of quercetin based chitosan-gelatin film for food packaging.
31590881	9	11	theme	film	1477:1480	arg1	Quercetin-starch					1493:1508	the film containing Quercetin-starch	1473:1508	the film containing Quercetin-starch presented superior antioxidant activity	1473:1548	It was observed that the film containing Quercetin-starch presented superior antioxidant activity results in comparison to Ch-ge film promising its application in food packaging.
31590881	6	12	from	increase	912:919	arg1	strength					932:939	tensile strength	924:939	tensile strength while a decrease in elongation at break has been observed in the Ch-ge-Q film	924:1017	The reduction of water-vapor permeability and increase in tensile strength while a decrease in elongation at break has been observed in the Ch-ge-Q film compared to Ch-ge film.
31590881	2	13	theme	chitosan-gelatin	328:343	arg1	characteristics					269:283	Physicochemical characteristics	253:283	Physicochemical characteristics	253:283	Physicochemical characteristics and mechanical properties of the resulting chitosan-gelatin containing Quercetin-starch films (Ch-ge-Q) were studied using UV, FTIR, XRD and SEM techniques.
31590881	2	13	theme	chitosan-gelatin	328:343	arg1	properties					300:309	mechanical properties	289:309	mechanical properties	289:309	Physicochemical characteristics and mechanical properties of the resulting chitosan-gelatin containing Quercetin-starch films (Ch-ge-Q) were studied using UV, FTIR, XRD and SEM techniques.
31590881	6	14	theme	Ch-ge-Q	1006:1012	arg1	film					1014:1017	the Ch-ge-Q film	1002:1017	the Ch-ge-Q film	1002:1017	The reduction of water-vapor permeability and increase in tensile strength while a decrease in elongation at break has been observed in the Ch-ge-Q film compared to Ch-ge film.
31590881	7	15	theme	loaded	1181:1186	arg1	films					1194:1198	the Q loaded Ch-ge films	1175:1198	the Q loaded Ch-ge films	1175:1198	The antibacterial activity of Ch-ge-Q film against both gram positive (B. substilis) and gram negative (E. coli) bacteria suggested the Q loaded Ch-ge films as more feasible antibacterial candidate especially against the strain E. coli.
31590881	1	16	contain	containing	173:182	arg2	Quercetin-starch					184:199	Quercetin-starch	184:199	Quercetin-starch (Q)	184:203	Ecofriendly chitosan-gelatin (Ch-ge) bio-composite films containing Quercetin-starch (Q) were synthesized using solution casting method.
31590881	1	16	contain	containing	173:182	arg1	films					167:171	Ecofriendly chitosan-gelatin (Ch-ge) bio-composite films	116:171	Ecofriendly chitosan-gelatin (Ch-ge) bio-composite films containing Quercetin-starch (Q)	116:203	Ecofriendly chitosan-gelatin (Ch-ge) bio-composite films containing Quercetin-starch (Q) were synthesized using solution casting method.
31590881	1	16	contain	containing	173:182	arg2	Q					202:202	Q	202:202	Q	202:202	Ecofriendly chitosan-gelatin (Ch-ge) bio-composite films containing Quercetin-starch (Q) were synthesized using solution casting method.
31590881	8	17	theme	antioxidant	1284:1294	arg1	activity					1296:1303	The antioxidant activity	1280:1303	The antioxidant activity of the Ch-ge-Q film	1280:1323	The antioxidant activity of the Ch-ge-Q film was evaluated using the DPPH and ABTS as standards and corresponded to 81.45% of DPPH and 72.2% of ABTS scavenging activities.
31590881	2	18	theme	resulting	318:326	arg1	chitosan-gelatin					328:343	the resulting chitosan-gelatin	314:343	the resulting chitosan-gelatin containing Quercetin-starch films (Ch-ge-Q)	314:387	Physicochemical characteristics and mechanical properties of the resulting chitosan-gelatin containing Quercetin-starch films (Ch-ge-Q) were studied using UV, FTIR, XRD and SEM techniques.
31590881	7	19	theme	Ch-ge	1188:1192	arg1	films					1194:1198	the Q loaded Ch-ge films	1175:1198	the Q loaded Ch-ge films	1175:1198	The antibacterial activity of Ch-ge-Q film against both gram positive (B. substilis) and gram negative (E. coli) bacteria suggested the Q loaded Ch-ge films as more feasible antibacterial candidate especially against the strain E. coli.
31590881	7	20	dep	positive	1104:1111	arg1	substilis					1117:1125	B. substilis	1114:1125	B. substilis	1114:1125	The antibacterial activity of Ch-ge-Q film against both gram positive (B. substilis) and gram negative (E. coli) bacteria suggested the Q loaded Ch-ge films as more feasible antibacterial candidate especially against the strain E. coli.
31590881	9	21	theme	containing	1482:1491	arg1	Quercetin-starch					1493:1508	the film containing Quercetin-starch	1473:1508	the film containing Quercetin-starch presented superior antioxidant activity	1473:1548	It was observed that the film containing Quercetin-starch presented superior antioxidant activity results in comparison to Ch-ge film promising its application in food packaging.
31590881	2	22	theme	SEM	426:428	arg1	techniques					430:439	UV, FTIR, XRD and SEM techniques	408:439	UV, FTIR, XRD and SEM techniques	408:439	Physicochemical characteristics and mechanical properties of the resulting chitosan-gelatin containing Quercetin-starch films (Ch-ge-Q) were studied using UV, FTIR, XRD and SEM techniques.
31590881	5	23	theme	barrier	832:838	arg1	property					840:847	enhanced barrier property	823:847	enhanced barrier property	823:847	The film also showed enhanced barrier property against UV rays.
31590881	7	24	theme	feasible	1208:1215	arg1	candidate					1231:1239	more feasible antibacterial candidate	1203:1239	more feasible antibacterial candidate especially against the strain E. coli	1203:1277	The antibacterial activity of Ch-ge-Q film against both gram positive (B. substilis) and gram negative (E. coli) bacteria suggested the Q loaded Ch-ge films as more feasible antibacterial candidate especially against the strain E. coli.
31590881	7	25	theme	positive	1104:1111	arg1	bacteria					1156:1163	both gram positive (B. substilis) and gram negative (E. coli) bacteria	1094:1163	both gram positive (B. substilis) and gram negative (E. coli) bacteria	1094:1163	The antibacterial activity of Ch-ge-Q film against both gram positive (B. substilis) and gram negative (E. coli) bacteria suggested the Q loaded Ch-ge films as more feasible antibacterial candidate especially against the strain E. coli.
31590881	7	26	theme	antibacterial	1217:1229	arg1	candidate					1231:1239	more feasible antibacterial candidate	1203:1239	more feasible antibacterial candidate especially against the strain E. coli	1203:1277	The antibacterial activity of Ch-ge-Q film against both gram positive (B. substilis) and gram negative (E. coli) bacteria suggested the Q loaded Ch-ge films as more feasible antibacterial candidate especially against the strain E. coli.
31590881	6	27	theme	tensile	924:930	arg1	strength					932:939	tensile strength	924:939	tensile strength while a decrease in elongation at break has been observed in the Ch-ge-Q film	924:1017	The reduction of water-vapor permeability and increase in tensile strength while a decrease in elongation at break has been observed in the Ch-ge-Q film compared to Ch-ge film.
31590881	7	28	theme	Q	1179:1179	arg1	films					1194:1198	the Q loaded Ch-ge films	1175:1198	the Q loaded Ch-ge films	1175:1198	The antibacterial activity of Ch-ge-Q film against both gram positive (B. substilis) and gram negative (E. coli) bacteria suggested the Q loaded Ch-ge films as more feasible antibacterial candidate especially against the strain E. coli.
31590881	4	29	theme	chemical	587:594	arg1	interactions					596:607	the chemical interactions	583:607	the chemical interactions between the chitosan-gelatin	583:636	The FTIR spectra confirmed the chemical interactions between the chitosan-gelatin and Q. Surface morphology of prepared film was analyzed by the SEM imaging while XRD spectra suggest the expanded crystallinity of the film with the addition of Q.
31590881	4	30	theme	film	773:776	arg1	crystallinity					752:764	the expanded crystallinity	739:764	the expanded crystallinity of the film	739:776	The FTIR spectra confirmed the chemical interactions between the chitosan-gelatin and Q. Surface morphology of prepared film was analyzed by the SEM imaging while XRD spectra suggest the expanded crystallinity of the film with the addition of Q.
31590881	9	31	from	application	1600:1610	arg1	packaging					1620:1628	food packaging	1615:1628	food packaging	1615:1628	It was observed that the film containing Quercetin-starch presented superior antioxidant activity results in comparison to Ch-ge film promising its application in food packaging.
31590881	2	32	theme	XRD	418:420	arg1	techniques					430:439	UV, FTIR, XRD and SEM techniques	408:439	UV, FTIR, XRD and SEM techniques	408:439	Physicochemical characteristics and mechanical properties of the resulting chitosan-gelatin containing Quercetin-starch films (Ch-ge-Q) were studied using UV, FTIR, XRD and SEM techniques.
31590881	9	33	theme	Ch-ge	1575:1579	arg1	application					1600:1610	Ch-ge film promising its application	1575:1610	Ch-ge film promising its application in food packaging	1575:1628	It was observed that the film containing Quercetin-starch presented superior antioxidant activity results in comparison to Ch-ge film promising its application in food packaging.
31590881	3	34	theme	water-vapor	495:505	arg1	WVP					521:523	WVP	521:523	WVP	521:523	The films were also investigated for their swelling, water-vapor permeability (WVP), water solubility properties.
31590881	3	34	theme	water-vapor	495:505	arg1	swelling					485:492	their swelling	479:492	their swelling	479:492	The films were also investigated for their swelling, water-vapor permeability (WVP), water solubility properties.
31590881	3	34	theme	water-vapor	495:505	arg1	permeability					507:518	water-vapor permeability	495:518	water-vapor permeability (WVP)	495:524	The films were also investigated for their swelling, water-vapor permeability (WVP), water solubility properties.
31590881	5	35	theme	UV	857:858	arg1	rays					860:863	UV rays	857:863	UV rays	857:863	The film also showed enhanced barrier property against UV rays.
31590881	8	36	theme	scavenging	1429:1438	arg1	activities					1440:1449	ABTS scavenging activities	1424:1449	ABTS scavenging activities	1424:1449	The antioxidant activity of the Ch-ge-Q film was evaluated using the DPPH and ABTS as standards and corresponded to 81.45% of DPPH and 72.2% of ABTS scavenging activities.
31590881	2	37	theme	Quercetin-starch	356:371	arg1	films					373:377	Quercetin-starch films	356:377	Quercetin-starch films (Ch-ge-Q)	356:387	Physicochemical characteristics and mechanical properties of the resulting chitosan-gelatin containing Quercetin-starch films (Ch-ge-Q) were studied using UV, FTIR, XRD and SEM techniques.
31590881	2	37	theme	Quercetin-starch	356:371	arg1	Ch-ge-Q					380:386	Ch-ge-Q	380:386	Ch-ge-Q	380:386	Physicochemical characteristics and mechanical properties of the resulting chitosan-gelatin containing Quercetin-starch films (Ch-ge-Q) were studied using UV, FTIR, XRD and SEM techniques.
31590881	9	38	theme	superior	1520:1527	arg1	activity					1541:1548	superior antioxidant activity	1520:1548	superior antioxidant activity	1520:1548	It was observed that the film containing Quercetin-starch presented superior antioxidant activity results in comparison to Ch-ge film promising its application in food packaging.
31590881	4	39	theme	film	676:679	arg1	morphology					653:662	Q. Surface morphology	642:662	Q. Surface morphology of prepared film	642:679	The FTIR spectra confirmed the chemical interactions between the chitosan-gelatin and Q. Surface morphology of prepared film was analyzed by the SEM imaging while XRD spectra suggest the expanded crystallinity of the film with the addition of Q.
31590881	7	40	dep	strain	1264:1269	arg1	coli					1274:1277	the strain E. coli	1260:1277	the strain E. coli	1260:1277	The antibacterial activity of Ch-ge-Q film against both gram positive (B. substilis) and gram negative (E. coli) bacteria suggested the Q loaded Ch-ge films as more feasible antibacterial candidate especially against the strain E. coli.
31590881	2	41	contain	containing	345:354	arg1	chitosan-gelatin					328:343	the resulting chitosan-gelatin	314:343	the resulting chitosan-gelatin containing Quercetin-starch films (Ch-ge-Q)	314:387	Physicochemical characteristics and mechanical properties of the resulting chitosan-gelatin containing Quercetin-starch films (Ch-ge-Q) were studied using UV, FTIR, XRD and SEM techniques.
31590881	2	41	contain	containing	345:354	arg2	Ch-ge-Q					380:386	Ch-ge-Q	380:386	Ch-ge-Q	380:386	Physicochemical characteristics and mechanical properties of the resulting chitosan-gelatin containing Quercetin-starch films (Ch-ge-Q) were studied using UV, FTIR, XRD and SEM techniques.
31590881	2	41	contain	containing	345:354	arg2	films					373:377	Quercetin-starch films	356:377	Quercetin-starch films (Ch-ge-Q)	356:387	Physicochemical characteristics and mechanical properties of the resulting chitosan-gelatin containing Quercetin-starch films (Ch-ge-Q) were studied using UV, FTIR, XRD and SEM techniques.
31590881	7	42	theme	film	1081:1084	arg1	activity					1061:1068	The antibacterial activity	1043:1068	The antibacterial activity of Ch-ge-Q film against both gram positive (B. substilis) and gram negative (E. coli) bacteria	1043:1163	The antibacterial activity of Ch-ge-Q film against both gram positive (B. substilis) and gram negative (E. coli) bacteria suggested the Q loaded Ch-ge films as more feasible antibacterial candidate especially against the strain E. coli.
31590881	6	43	theme	permeability	895:906	arg1	increase					912:919	increase	912:919	increase in tensile strength while a decrease in elongation at break has been observed in the Ch-ge-Q film	912:1017	The reduction of water-vapor permeability and increase in tensile strength while a decrease in elongation at break has been observed in the Ch-ge-Q film compared to Ch-ge film.
31590881	6	43	theme	permeability	895:906	arg1	reduction					870:878	The reduction	866:878	The reduction of water-vapor permeability	866:906	The reduction of water-vapor permeability and increase in tensile strength while a decrease in elongation at break has been observed in the Ch-ge-Q film compared to Ch-ge film.
31590881	9	44	theme	promising	1586:1594	arg1	application					1600:1610	Ch-ge film promising its application	1575:1610	Ch-ge film promising its application in food packaging	1575:1628	It was observed that the film containing Quercetin-starch presented superior antioxidant activity results in comparison to Ch-ge film promising its application in food packaging.
31590881	1	45	theme	solution	228:235	arg1	method					245:250	solution casting method	228:250	solution casting method	228:250	Ecofriendly chitosan-gelatin (Ch-ge) bio-composite films containing Quercetin-starch (Q) were synthesized using solution casting method.
31590881	0	46	theme	biological	33:42	arg1	evaluation					44:53	biological evaluation	33:53	biological evaluation	33:53	Preparation, physicochemical and biological evaluation of quercetin based chitosan-gelatin film for food packaging.
31590881	4	47	theme	FTIR	560:563	arg1	spectra					565:571	The FTIR spectra	556:571	The FTIR spectra	556:571	The FTIR spectra confirmed the chemical interactions between the chitosan-gelatin and Q. Surface morphology of prepared film was analyzed by the SEM imaging while XRD spectra suggest the expanded crystallinity of the film with the addition of Q.
31590881	6	48	theme	water-vapor	883:893	arg1	permeability					895:906	water-vapor permeability	883:906	water-vapor permeability	883:906	The reduction of water-vapor permeability and increase in tensile strength while a decrease in elongation at break has been observed in the Ch-ge-Q film compared to Ch-ge film.
31590881	2	49	theme	Physicochemical	253:267	arg1	characteristics					269:283	Physicochemical characteristics	253:283	Physicochemical characteristics	253:283	Physicochemical characteristics and mechanical properties of the resulting chitosan-gelatin containing Quercetin-starch films (Ch-ge-Q) were studied using UV, FTIR, XRD and SEM techniques.
31590881	1	50	theme	casting	237:243	arg1	method					245:250	solution casting method	228:250	solution casting method	228:250	Ecofriendly chitosan-gelatin (Ch-ge) bio-composite films containing Quercetin-starch (Q) were synthesized using solution casting method.
31590881	0	51	theme	quercetin	58:66	arg1	physicochemical					13:27	physicochemical	13:27	physicochemical	13:27	Preparation, physicochemical and biological evaluation of quercetin based chitosan-gelatin film for food packaging.
31590881	0	51	theme	quercetin	58:66	arg1	evaluation					44:53	biological evaluation	33:53	biological evaluation	33:53	Preparation, physicochemical and biological evaluation of quercetin based chitosan-gelatin film for food packaging.
31590881	0	51	theme	quercetin	58:66	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation, physicochemical and biological evaluation of quercetin based chitosan-gelatin film for food packaging.
31590881	8	52	theme	ABTS	1424:1427	arg1	activities					1440:1449	ABTS scavenging activities	1424:1449	ABTS scavenging activities	1424:1449	The antioxidant activity of the Ch-ge-Q film was evaluated using the DPPH and ABTS as standards and corresponded to 81.45% of DPPH and 72.2% of ABTS scavenging activities.
31590881	1	53	theme	chitosan-gelatin	128:143	arg1	films					167:171	Ecofriendly chitosan-gelatin (Ch-ge) bio-composite films	116:171	Ecofriendly chitosan-gelatin (Ch-ge) bio-composite films containing Quercetin-starch (Q)	116:203	Ecofriendly chitosan-gelatin (Ch-ge) bio-composite films containing Quercetin-starch (Q) were synthesized using solution casting method.
31590881	5	54	theme	enhanced	823:830	arg1	property					840:847	enhanced barrier property	823:847	enhanced barrier property	823:847	The film also showed enhanced barrier property against UV rays.
31590881	7	55	theme	Ch-ge-Q	1073:1079	arg1	film					1081:1084	Ch-ge-Q film	1073:1084	Ch-ge-Q film	1073:1084	The antibacterial activity of Ch-ge-Q film against both gram positive (B. substilis) and gram negative (E. coli) bacteria suggested the Q loaded Ch-ge films as more feasible antibacterial candidate especially against the strain E. coli.
31590881	9	56	theme	antioxidant	1529:1539	arg1	activity					1541:1548	superior antioxidant activity	1520:1548	superior antioxidant activity	1520:1548	It was observed that the film containing Quercetin-starch presented superior antioxidant activity results in comparison to Ch-ge film promising its application in food packaging.
31590881	8	57	theme	activities	1440:1449	arg1	DPPH					1406:1409	DPPH	1406:1409	DPPH	1406:1409	The antioxidant activity of the Ch-ge-Q film was evaluated using the DPPH and ABTS as standards and corresponded to 81.45% of DPPH and 72.2% of ABTS scavenging activities.
31590881	8	57	theme	activities	1440:1449	arg1	%					1419:1419	72.2%	1415:1419	72.2% of ABTS scavenging activities	1415:1449	The antioxidant activity of the Ch-ge-Q film was evaluated using the DPPH and ABTS as standards and corresponded to 81.45% of DPPH and 72.2% of ABTS scavenging activities.
31590881	8	57	theme	activities	1440:1449	arg1	%					1401:1401	81.45%	1396:1401	81.45% of DPPH	1396:1409	The antioxidant activity of the Ch-ge-Q film was evaluated using the DPPH and ABTS as standards and corresponded to 81.45% of DPPH and 72.2% of ABTS scavenging activities.
31590881	8	57	theme	activities	1440:1449	arg1	activities					1440:1449	ABTS scavenging activities	1424:1449	ABTS scavenging activities	1424:1449	The antioxidant activity of the Ch-ge-Q film was evaluated using the DPPH and ABTS as standards and corresponded to 81.45% of DPPH and 72.2% of ABTS scavenging activities.
31590881	3	58	theme	solubility	533:542	arg1	properties					544:553	water solubility properties	527:553	water solubility properties	527:553	The films were also investigated for their swelling, water-vapor permeability (WVP), water solubility properties.
31590881	3	58	theme	solubility	533:542	arg1	swelling					485:492	their swelling	479:492	their swelling	479:492	The films were also investigated for their swelling, water-vapor permeability (WVP), water solubility properties.
31590881	4	59	theme	expanded	743:750	arg1	crystallinity					752:764	the expanded crystallinity	739:764	the expanded crystallinity of the film	739:776	The FTIR spectra confirmed the chemical interactions between the chitosan-gelatin and Q. Surface morphology of prepared film was analyzed by the SEM imaging while XRD spectra suggest the expanded crystallinity of the film with the addition of Q.
31590881	7	60	theme	antibacterial	1047:1059	arg1	activity					1061:1068	The antibacterial activity	1043:1068	The antibacterial activity of Ch-ge-Q film against both gram positive (B. substilis) and gram negative (E. coli) bacteria	1043:1163	The antibacterial activity of Ch-ge-Q film against both gram positive (B. substilis) and gram negative (E. coli) bacteria suggested the Q loaded Ch-ge films as more feasible antibacterial candidate especially against the strain E. coli.
31590881	6	61	dep	strength	932:939	arg1	observed					990:997	observed	990:997	has been observed in the Ch-ge-Q film	981:1017	The reduction of water-vapor permeability and increase in tensile strength while a decrease in elongation at break has been observed in the Ch-ge-Q film compared to Ch-ge film.
31590881	8	62	theme	DPPH	1406:1409	arg1	DPPH					1406:1409	DPPH	1406:1409	DPPH	1406:1409	The antioxidant activity of the Ch-ge-Q film was evaluated using the DPPH and ABTS as standards and corresponded to 81.45% of DPPH and 72.2% of ABTS scavenging activities.
31590881	8	62	theme	DPPH	1406:1409	arg1	%					1419:1419	72.2%	1415:1419	72.2% of ABTS scavenging activities	1415:1449	The antioxidant activity of the Ch-ge-Q film was evaluated using the DPPH and ABTS as standards and corresponded to 81.45% of DPPH and 72.2% of ABTS scavenging activities.
31590881	8	62	theme	DPPH	1406:1409	arg1	%					1401:1401	81.45%	1396:1401	81.45% of DPPH	1396:1409	The antioxidant activity of the Ch-ge-Q film was evaluated using the DPPH and ABTS as standards and corresponded to 81.45% of DPPH and 72.2% of ABTS scavenging activities.
31590881	8	62	theme	DPPH	1406:1409	arg1	activities					1440:1449	ABTS scavenging activities	1424:1449	ABTS scavenging activities	1424:1449	The antioxidant activity of the Ch-ge-Q film was evaluated using the DPPH and ABTS as standards and corresponded to 81.45% of DPPH and 72.2% of ABTS scavenging activities.
31590881	1	63	theme	Ch-ge	146:150	arg1	films					167:171	Ecofriendly chitosan-gelatin (Ch-ge) bio-composite films	116:171	Ecofriendly chitosan-gelatin (Ch-ge) bio-composite films containing Quercetin-starch (Q)	116:203	Ecofriendly chitosan-gelatin (Ch-ge) bio-composite films containing Quercetin-starch (Q) were synthesized using solution casting method.
31590881	6	64	from	break	975:979	arg1	elongation					961:970	elongation	961:970	elongation at break	961:979	The reduction of water-vapor permeability and increase in tensile strength while a decrease in elongation at break has been observed in the Ch-ge-Q film compared to Ch-ge film.
31590881	6	64	from	break	975:979	arg1	decrease					949:956	a decrease	947:956	a decrease in elongation at break	947:979	The reduction of water-vapor permeability and increase in tensile strength while a decrease in elongation at break has been observed in the Ch-ge-Q film compared to Ch-ge film.
31590881	8	65	theme	film	1320:1323	arg1	activity					1296:1303	The antioxidant activity	1280:1303	The antioxidant activity of the Ch-ge-Q film	1280:1323	The antioxidant activity of the Ch-ge-Q film was evaluated using the DPPH and ABTS as standards and corresponded to 81.45% of DPPH and 72.2% of ABTS scavenging activities.
31590881	4	66	theme	XRD	719:721	arg1	spectra					723:729	XRD spectra	719:729	XRD spectra	719:729	The FTIR spectra confirmed the chemical interactions between the chitosan-gelatin and Q. Surface morphology of prepared film was analyzed by the SEM imaging while XRD spectra suggest the expanded crystallinity of the film with the addition of Q.
31590881	4	67	theme	Surface	645:651	arg1	morphology					653:662	Q. Surface morphology	642:662	Q. Surface morphology of prepared film	642:679	The FTIR spectra confirmed the chemical interactions between the chitosan-gelatin and Q. Surface morphology of prepared film was analyzed by the SEM imaging while XRD spectra suggest the expanded crystallinity of the film with the addition of Q.
31590881	0	68	theme	chitosan-gelatin	74:89	arg1	film					91:94	chitosan-gelatin film	74:94	chitosan-gelatin film for food packaging	74:113	Preparation, physicochemical and biological evaluation of quercetin based chitosan-gelatin film for food packaging.
31590881	2	69	theme	mechanical	289:298	arg1	properties					300:309	mechanical properties	289:309	mechanical properties	289:309	Physicochemical characteristics and mechanical properties of the resulting chitosan-gelatin containing Quercetin-starch films (Ch-ge-Q) were studied using UV, FTIR, XRD and SEM techniques.
31590881	9	70	theme	food	1615:1618	arg1	packaging					1620:1628	food packaging	1615:1628	food packaging	1615:1628	It was observed that the film containing Quercetin-starch presented superior antioxidant activity results in comparison to Ch-ge film promising its application in food packaging.
31590881	1	71	theme	bio-composite	153:165	arg1	films					167:171	Ecofriendly chitosan-gelatin (Ch-ge) bio-composite films	116:171	Ecofriendly chitosan-gelatin (Ch-ge) bio-composite films containing Quercetin-starch (Q)	116:203	Ecofriendly chitosan-gelatin (Ch-ge) bio-composite films containing Quercetin-starch (Q) were synthesized using solution casting method.
31590881	4	72	theme	prepared	667:674	arg1	film					676:679	prepared film	667:679	prepared film	667:679	The FTIR spectra confirmed the chemical interactions between the chitosan-gelatin and Q. Surface morphology of prepared film was analyzed by the SEM imaging while XRD spectra suggest the expanded crystallinity of the film with the addition of Q.
31590881	6	73	from	reduction	870:878	arg1	strength					932:939	tensile strength	924:939	tensile strength while a decrease in elongation at break has been observed in the Ch-ge-Q film	924:1017	The reduction of water-vapor permeability and increase in tensile strength while a decrease in elongation at break has been observed in the Ch-ge-Q film compared to Ch-ge film.
31590881	6	74	located	observed	990:997	arg2	decrease					949:956	a decrease	947:956	a decrease in elongation at break	947:979	The reduction of water-vapor permeability and increase in tensile strength while a decrease in elongation at break has been observed in the Ch-ge-Q film compared to Ch-ge film.
31590881	6	74	located	observed	990:997	arg1	film					1014:1017	the Ch-ge-Q film	1002:1017	the Ch-ge-Q film	1002:1017	The reduction of water-vapor permeability and increase in tensile strength while a decrease in elongation at break has been observed in the Ch-ge-Q film compared to Ch-ge film.
31590881	7	75	theme	E.	1271:1272	arg1	strain					1264:1269	the strain E. coli	1260:1277	the strain E. coli	1260:1277	The antibacterial activity of Ch-ge-Q film against both gram positive (B. substilis) and gram negative (E. coli) bacteria suggested the Q loaded Ch-ge films as more feasible antibacterial candidate especially against the strain E. coli.
31590881	2	76	theme	FTIR	412:415	arg1	techniques					430:439	UV, FTIR, XRD and SEM techniques	408:439	UV, FTIR, XRD and SEM techniques	408:439	Physicochemical characteristics and mechanical properties of the resulting chitosan-gelatin containing Quercetin-starch films (Ch-ge-Q) were studied using UV, FTIR, XRD and SEM techniques.
31590881	6	77	theme	Ch-ge	1031:1035	arg1	film					1037:1040	Ch-ge film	1031:1040	Ch-ge film	1031:1040	The reduction of water-vapor permeability and increase in tensile strength while a decrease in elongation at break has been observed in the Ch-ge-Q film compared to Ch-ge film.
30511915	5	0	located	observed	555:562	arg1	media					571:575	the media	567:575	the media tested	567:582	Substrate mycelia and aerial mycelia were not formed, and no diffusible pigments were observed on the media tested.
30511915	5	0	located	observed	555:562	arg2	pigments					541:548	no diffusible pigments	527:548	no diffusible pigments	527:548	Substrate mycelia and aerial mycelia were not formed, and no diffusible pigments were observed on the media tested.
30511915	9	1	theme	unidentified	1121:1132	arg1	phospholipid					1134:1145	an unidentified phospholipid	1118:1145	an unidentified phospholipid	1118:1145	The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, an unidentified glycolipid, an unidentified phospholipid and an unidentified lipid, while the major fatty acids were iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0.
30511915	10	2	theme	name	1430:1433	arg1	sp					1455:1456	the name Amnibacteriumflavum sp	1426:1456	the name Amnibacteriumflavum sp	1426:1456	On the basis of phylogenetic, chemotaxonomic and phenotypic data, strain M8JJ-5T can be characterized to represent a novel species of the genus Amnibacterium, for which the name Amnibacteriumflavum sp.
30511915	6	3	theme	type	702:705	arg1	strains					707:713	the type strains	698:713	the type strains of genus Amnibacterium	698:736	Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain M8JJ-5T was most closely related to the type strains of genus Amnibacterium, and shared highest 16S rRNA gene sequence similarity of 97.29 % to Amnibacterium kyonggiense KSL51201-037T.
30511915	7	4	theme	M8JJ-5T	877:883	arg1	G+C content					855:865	The DNA G+C content	847:865	The DNA G+C content of strain M8JJ-5T	847:883	The DNA G+C content of strain M8JJ-5T was 68.6 mol%.
30511915	7	4	theme	M8JJ-5T	877:883	arg1	%					897:897	68.6 mol%	889:897	68.6 mol%	889:897	The DNA G+C content of strain M8JJ-5T was 68.6 mol%.
30511915	6	5	theme	16S	620:622	arg1	rRNA					624:627	the 16S rRNA	616:627	the 16S rRNA gene sequence	616:641	Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain M8JJ-5T was most closely related to the type strains of genus Amnibacterium, and shared highest 16S rRNA gene sequence similarity of 97.29 % to Amnibacterium kyonggiense KSL51201-037T.
30511915	12	6	theme	=KCTC	1505:1509	arg1	1.16390T					1524:1531	=KCTC 49089T=CGMCC 1.16390T	1505:1531	=KCTC 49089T=CGMCC 1.16390T	1505:1531	The type strain is M8JJ-5T (=KCTC 49089T=CGMCC 1.16390T).
30511915	12	6	theme	=KCTC	1505:1509	arg1	M8JJ-5T					1496:1502	M8JJ-5T	1496:1502	M8JJ-5T (=KCTC 49089T=CGMCC 1.16390T)	1496:1532	The type strain is M8JJ-5T (=KCTC 49089T=CGMCC 1.16390T).
30511915	9	7	dep	 0	1216:1217	arg1	anteiso-C17 					1240:1251	anteiso-C17 	1240:1251	anteiso-C17 	1240:1251	The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, an unidentified glycolipid, an unidentified phospholipid and an unidentified lipid, while the major fatty acids were iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0.
30511915	9	7	dep	 0	1216:1217	arg1	 0					1233:1234	 0	1233:1234	 0	1233:1234	The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, an unidentified glycolipid, an unidentified phospholipid and an unidentified lipid, while the major fatty acids were iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0.
30511915	9	7	dep	 0	1216:1217	arg1	 0					1253:1254	 0	1253:1254	 0	1253:1254	The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, an unidentified glycolipid, an unidentified phospholipid and an unidentified lipid, while the major fatty acids were iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0.
30511915	8	8	theme	major	990:994	arg1	menaquinones					996:1007	the major menaquinones	986:1007	the major menaquinones	986:1007	The cell-wall peptidoglycan contained l-2,4-diaminobutyric acid and MK-12, MK-11 were the major menaquinones.
30511915	8	8	theme	major	990:994	arg1	MK-12					968:972	MK-12	968:972	MK-12	968:972	The cell-wall peptidoglycan contained l-2,4-diaminobutyric acid and MK-12, MK-11 were the major menaquinones.
30511915	8	9	theme	l-2,4-diaminobutyric	938:957	arg1	acid					959:962	l-2,4-diaminobutyric acid	938:962	l-2,4-diaminobutyric acid	938:962	The cell-wall peptidoglycan contained l-2,4-diaminobutyric acid and MK-12, MK-11 were the major menaquinones.
30511915	4	10	theme	Strain	398:403	arg1	M8JJ-5T					405:411	Strain M8JJ-5T	398:411	Strain M8JJ-5T	398:411	Strain M8JJ-5T grew optimally without NaCl at 28 °C and at pH 7.0-8.0.
30511915	1	11	theme	endophytic	39:48	arg1	actinobacterium					50:64	a novel endophytic actinobacterium	31:64	a novel endophytic actinobacterium isolated from bark of Nerium indicum Mill	31:106	nov., a novel endophytic actinobacterium isolated from bark of Nerium indicum Mill.
30511915	9	12	theme	fatty	1190:1194	arg1	iso-C16 					1207:1214	iso-C16 	1207:1214	iso-C16 	1207:1214	The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, an unidentified glycolipid, an unidentified phospholipid and an unidentified lipid, while the major fatty acids were iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0.
30511915	9	12	theme	fatty	1190:1194	arg1	acids					1196:1200	the major fatty acids	1180:1200	the major fatty acids	1180:1200	The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, an unidentified glycolipid, an unidentified phospholipid and an unidentified lipid, while the major fatty acids were iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0.
30511915	10	13	theme	phenotypic	1306:1315	arg1	data					1317:1320	phylogenetic, chemotaxonomic and phenotypic data	1273:1320	phylogenetic, chemotaxonomic and phenotypic data	1273:1320	On the basis of phylogenetic, chemotaxonomic and phenotypic data, strain M8JJ-5T can be characterized to represent a novel species of the genus Amnibacterium, for which the name Amnibacteriumflavum sp.
30511915	10	14	theme	novel	1374:1378	arg1	species					1380:1386	a novel species	1372:1386	a novel species	1372:1386	On the basis of phylogenetic, chemotaxonomic and phenotypic data, strain M8JJ-5T can be characterized to represent a novel species of the genus Amnibacterium, for which the name Amnibacteriumflavum sp.
30511915	9	15	theme	unidentified	1093:1104	arg1	glycolipid					1106:1115	an unidentified glycolipid	1090:1115	an unidentified glycolipid	1090:1115	The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, an unidentified glycolipid, an unidentified phospholipid and an unidentified lipid, while the major fatty acids were iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0.
30511915	5	16	theme	aerial	491:496	arg1	mycelia					498:504	aerial mycelia	491:504	aerial mycelia	491:504	Substrate mycelia and aerial mycelia were not formed, and no diffusible pigments were observed on the media tested.
30511915	2	17	attach	isolated	225:232	arg1	bark					260:263	a surface-sterilized bark	239:263	a surface-sterilized bark of Neriumindicum Mill	239:285	A Gram-stain-positive, aerobic, short-rod-shaped, non-spore-forming actinobacterial strain, designated M8JJ-5T, was isolated from a surface-sterilized bark of Neriumindicum Mill.
30511915	2	17	attach	isolated	225:232	arg2	strain					193:198	A Gram-stain-positive, aerobic, short-rod-shaped, non-spore-forming actinobacterial strain	109:198	A Gram-stain-positive, aerobic, short-rod-shaped, non-spore-forming actinobacterial strain	109:198	A Gram-stain-positive, aerobic, short-rod-shaped, non-spore-forming actinobacterial strain, designated M8JJ-5T, was isolated from a surface-sterilized bark of Neriumindicum Mill.
30511915	3	18	theme	polyphasic	341:350	arg1	approach					352:359	a polyphasic approach	339:359	a polyphasic approach to determine its taxonomic position	339:395	collected from Guizhou, China, and investigated by a polyphasic approach to determine its taxonomic position.
30511915	6	19	theme	sequence	772:779	arg1	similarity					781:790	highest 16S rRNA gene sequence similarity	750:790	highest 16S rRNA gene sequence similarity of 97.29 % to Amnibacterium kyonggiense KSL51201-037T	750:844	Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain M8JJ-5T was most closely related to the type strains of genus Amnibacterium, and shared highest 16S rRNA gene sequence similarity of 97.29 % to Amnibacterium kyonggiense KSL51201-037T.
30511915	7	20	theme	DNA	851:853	arg1	G+C content					855:865	The DNA G+C content	847:865	The DNA G+C content of strain M8JJ-5T	847:883	The DNA G+C content of strain M8JJ-5T was 68.6 mol%.
30511915	7	20	theme	DNA	851:853	arg1	%					897:897	68.6 mol%	889:897	68.6 mol%	889:897	The DNA G+C content of strain M8JJ-5T was 68.6 mol%.
30511915	12	21	theme	49089T=CGMCC	1511:1522	arg1	1.16390T					1524:1531	=KCTC 49089T=CGMCC 1.16390T	1505:1531	=KCTC 49089T=CGMCC 1.16390T	1505:1531	The type strain is M8JJ-5T (=KCTC 49089T=CGMCC 1.16390T).
30511915	12	21	theme	49089T=CGMCC	1511:1522	arg1	M8JJ-5T					1496:1502	M8JJ-5T	1496:1502	M8JJ-5T (=KCTC 49089T=CGMCC 1.16390T)	1496:1532	The type strain is M8JJ-5T (=KCTC 49089T=CGMCC 1.16390T).
30511915	6	22	theme	gene	767:770	arg1	similarity					781:790	highest 16S rRNA gene sequence similarity	750:790	highest 16S rRNA gene sequence similarity of 97.29 % to Amnibacterium kyonggiense KSL51201-037T	750:844	Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain M8JJ-5T was most closely related to the type strains of genus Amnibacterium, and shared highest 16S rRNA gene sequence similarity of 97.29 % to Amnibacterium kyonggiense KSL51201-037T.
30511915	2	23	theme	Mill	282:285	arg1	bark					260:263	a surface-sterilized bark	239:263	a surface-sterilized bark of Neriumindicum Mill	239:285	A Gram-stain-positive, aerobic, short-rod-shaped, non-spore-forming actinobacterial strain, designated M8JJ-5T, was isolated from a surface-sterilized bark of Neriumindicum Mill.
30511915	0	24	theme	flavum	14:19	arg1	sp					21:22	Amnibacterium flavum sp	0:22	Amnibacterium flavum sp.	0:23	Amnibacterium flavum sp.
30511915	6	25	theme	rRNA	762:765	arg1	similarity					781:790	highest 16S rRNA gene sequence similarity	750:790	highest 16S rRNA gene sequence similarity of 97.29 % to Amnibacterium kyonggiense KSL51201-037T	750:844	Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain M8JJ-5T was most closely related to the type strains of genus Amnibacterium, and shared highest 16S rRNA gene sequence similarity of 97.29 % to Amnibacterium kyonggiense KSL51201-037T.
30511915	0	26	theme	Amnibacterium	0:12	arg1	sp					21:22	Amnibacterium flavum sp	0:22	Amnibacterium flavum sp.	0:23	Amnibacterium flavum sp.
30511915	10	27	theme	Amnibacteriumflavum	1435:1453	arg1	sp					1455:1456	the name Amnibacteriumflavum sp	1426:1456	the name Amnibacteriumflavum sp	1426:1456	On the basis of phylogenetic, chemotaxonomic and phenotypic data, strain M8JJ-5T can be characterized to represent a novel species of the genus Amnibacterium, for which the name Amnibacteriumflavum sp.
30511915	6	28	theme	16S	758:760	arg1	similarity					781:790	highest 16S rRNA gene sequence similarity	750:790	highest 16S rRNA gene sequence similarity of 97.29 % to Amnibacterium kyonggiense KSL51201-037T	750:844	Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain M8JJ-5T was most closely related to the type strains of genus Amnibacterium, and shared highest 16S rRNA gene sequence similarity of 97.29 % to Amnibacterium kyonggiense KSL51201-037T.
30511915	12	29	theme	type	1481:1484	arg1	strain					1486:1491	The type strain	1477:1491	The type strain	1477:1491	The type strain is M8JJ-5T (=KCTC 49089T=CGMCC 1.16390T).
30511915	12	29	theme	type	1481:1484	arg1	M8JJ-5T					1496:1502	M8JJ-5T	1496:1502	M8JJ-5T (=KCTC 49089T=CGMCC 1.16390T)	1496:1532	The type strain is M8JJ-5T (=KCTC 49089T=CGMCC 1.16390T).
30511915	6	30	theme	Phylogenetic	585:596	arg1	analysis					598:605	Phylogenetic analysis	585:605	Phylogenetic analysis based on the 16S rRNA gene sequence	585:641	Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain M8JJ-5T was most closely related to the type strains of genus Amnibacterium, and shared highest 16S rRNA gene sequence similarity of 97.29 % to Amnibacterium kyonggiense KSL51201-037T.
30511915	9	31	dep	diphosphatidylglycerol	1044:1065	arg1	 0					1216:1217	 0	1216:1217	 0	1216:1217	The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, an unidentified glycolipid, an unidentified phospholipid and an unidentified lipid, while the major fatty acids were iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0.
30511915	6	32	theme	highest	750:756	arg1	similarity					781:790	highest 16S rRNA gene sequence similarity	750:790	highest 16S rRNA gene sequence similarity of 97.29 % to Amnibacterium kyonggiense KSL51201-037T	750:844	Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain M8JJ-5T was most closely related to the type strains of genus Amnibacterium, and shared highest 16S rRNA gene sequence similarity of 97.29 % to Amnibacterium kyonggiense KSL51201-037T.
30511915	1	33	theme	Nerium	88:93	arg1	Mill					103:106	Nerium indicum Mill	88:106	Nerium indicum Mill	88:106	nov., a novel endophytic actinobacterium isolated from bark of Nerium indicum Mill.
30511915	5	34	theme	Substrate	469:477	arg1	mycelia					479:485	Substrate mycelia	469:485	Substrate mycelia	469:485	Substrate mycelia and aerial mycelia were not formed, and no diffusible pigments were observed on the media tested.
30511915	3	35	theme	taxonomic	378:386	arg1	position					388:395	its taxonomic position	374:395	its taxonomic position	374:395	collected from Guizhou, China, and investigated by a polyphasic approach to determine its taxonomic position.
30511915	10	36	dep	data	1317:1320	arg1	basis					1264:1268	basis	1264:1268	basis	1264:1268	On the basis of phylogenetic, chemotaxonomic and phenotypic data, strain M8JJ-5T can be characterized to represent a novel species of the genus Amnibacterium, for which the name Amnibacteriumflavum sp.
30511915	10	36	dep	data	1317:1320	arg1	the					1260:1262	the	1260:1262	the	1260:1262	On the basis of phylogenetic, chemotaxonomic and phenotypic data, strain M8JJ-5T can be characterized to represent a novel species of the genus Amnibacterium, for which the name Amnibacteriumflavum sp.
30511915	1	37	theme	indicum	95:101	arg1	Mill					103:106	Nerium indicum Mill	88:106	Nerium indicum Mill	88:106	nov., a novel endophytic actinobacterium isolated from bark of Nerium indicum Mill.
30511915	10	38	theme	phylogenetic	1273:1284	arg1	data					1317:1320	phylogenetic, chemotaxonomic and phenotypic data	1273:1320	phylogenetic, chemotaxonomic and phenotypic data	1273:1320	On the basis of phylogenetic, chemotaxonomic and phenotypic data, strain M8JJ-5T can be characterized to represent a novel species of the genus Amnibacterium, for which the name Amnibacteriumflavum sp.
30511915	10	39	theme	Amnibacterium	1401:1413	arg1	species					1380:1386	a novel species	1372:1386	a novel species	1372:1386	On the basis of phylogenetic, chemotaxonomic and phenotypic data, strain M8JJ-5T can be characterized to represent a novel species of the genus Amnibacterium, for which the name Amnibacteriumflavum sp.
30511915	1	40	attach	isolated	66:73	arg1	bark					80:83	bark	80:83	bark of Nerium indicum Mill	80:106	nov., a novel endophytic actinobacterium isolated from bark of Nerium indicum Mill.
30511915	1	40	attach	isolated	66:73	arg2	actinobacterium					50:64	a novel endophytic actinobacterium	31:64	a novel endophytic actinobacterium isolated from bark of Nerium indicum Mill	31:106	nov., a novel endophytic actinobacterium isolated from bark of Nerium indicum Mill.
30511915	2	41	theme	actinobacterial	177:191	arg1	strain					193:198	A Gram-stain-positive, aerobic, short-rod-shaped, non-spore-forming actinobacterial strain	109:198	A Gram-stain-positive, aerobic, short-rod-shaped, non-spore-forming actinobacterial strain	109:198	A Gram-stain-positive, aerobic, short-rod-shaped, non-spore-forming actinobacterial strain, designated M8JJ-5T, was isolated from a surface-sterilized bark of Neriumindicum Mill.
30511915	6	42	theme	strain	655:660	arg1	M8JJ-5T					662:668	strain M8JJ-5T	655:668	strain M8JJ-5T	655:668	Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain M8JJ-5T was most closely related to the type strains of genus Amnibacterium, and shared highest 16S rRNA gene sequence similarity of 97.29 % to Amnibacterium kyonggiense KSL51201-037T.
30511915	7	43	theme	68.6 mol	889:896	arg1	G+C content					855:865	The DNA G+C content	847:865	The DNA G+C content of strain M8JJ-5T	847:883	The DNA G+C content of strain M8JJ-5T was 68.6 mol%.
30511915	7	43	theme	68.6 mol	889:896	arg1	%					897:897	68.6 mol%	889:897	68.6 mol%	889:897	The DNA G+C content of strain M8JJ-5T was 68.6 mol%.
30511915	5	44	theme	diffusible	530:539	arg1	pigments					541:548	no diffusible pigments	527:548	no diffusible pigments	527:548	Substrate mycelia and aerial mycelia were not formed, and no diffusible pigments were observed on the media tested.
30511915	8	45	contain	contained	928:936	arg1	peptidoglycan					914:926	The cell-wall peptidoglycan	900:926	The cell-wall peptidoglycan	900:926	The cell-wall peptidoglycan contained l-2,4-diaminobutyric acid and MK-12, MK-11 were the major menaquinones.
30511915	8	45	contain	contained	928:936	arg2	acid					959:962	l-2,4-diaminobutyric acid	938:962	l-2,4-diaminobutyric acid	938:962	The cell-wall peptidoglycan contained l-2,4-diaminobutyric acid and MK-12, MK-11 were the major menaquinones.
30511915	10	46	theme	chemotaxonomic	1287:1300	arg1	data					1317:1320	phylogenetic, chemotaxonomic and phenotypic data	1273:1320	phylogenetic, chemotaxonomic and phenotypic data	1273:1320	On the basis of phylogenetic, chemotaxonomic and phenotypic data, strain M8JJ-5T can be characterized to represent a novel species of the genus Amnibacterium, for which the name Amnibacteriumflavum sp.
30511915	4	47	theme	pH	457:458	arg1	7.0-8.0					460:466	pH 7.0-8.0	457:466	pH 7.0-8.0	457:466	Strain M8JJ-5T grew optimally without NaCl at 28 °C and at pH 7.0-8.0.
30511915	9	48	theme	unidentified	1154:1165	arg1	lipid					1167:1171	an unidentified lipid	1151:1171	an unidentified lipid	1151:1171	The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, an unidentified glycolipid, an unidentified phospholipid and an unidentified lipid, while the major fatty acids were iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0.
30511915	1	49	theme	Mill	103:106	arg1	bark					80:83	bark	80:83	bark of Nerium indicum Mill	80:106	nov., a novel endophytic actinobacterium isolated from bark of Nerium indicum Mill.
30511915	2	50	dep	Gram-stain-positive	111:129	arg1	aerobic					132:138	aerobic	132:138	aerobic	132:138	A Gram-stain-positive, aerobic, short-rod-shaped, non-spore-forming actinobacterial strain, designated M8JJ-5T, was isolated from a surface-sterilized bark of Neriumindicum Mill.
30511915	2	50	dep	Gram-stain-positive	111:129	arg1	non-spore-forming					159:175	non-spore-forming	159:175	non-spore-forming	159:175	A Gram-stain-positive, aerobic, short-rod-shaped, non-spore-forming actinobacterial strain, designated M8JJ-5T, was isolated from a surface-sterilized bark of Neriumindicum Mill.
30511915	2	50	dep	Gram-stain-positive	111:129	arg1	short-rod-shaped					141:156	short-rod-shaped	141:156	short-rod-shaped	141:156	A Gram-stain-positive, aerobic, short-rod-shaped, non-spore-forming actinobacterial strain, designated M8JJ-5T, was isolated from a surface-sterilized bark of Neriumindicum Mill.
30511915	6	51	theme	kyonggiense	820:830	arg1	KSL51201-037T					832:844	Amnibacterium kyonggiense KSL51201-037T	806:844	Amnibacterium kyonggiense KSL51201-037T	806:844	Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain M8JJ-5T was most closely related to the type strains of genus Amnibacterium, and shared highest 16S rRNA gene sequence similarity of 97.29 % to Amnibacterium kyonggiense KSL51201-037T.
30511915	10	52	theme	genus	1395:1399	arg1	Amnibacterium					1401:1413	the genus Amnibacterium	1391:1413	the genus Amnibacterium	1391:1413	On the basis of phylogenetic, chemotaxonomic and phenotypic data, strain M8JJ-5T can be characterized to represent a novel species of the genus Amnibacterium, for which the name Amnibacteriumflavum sp.
30511915	10	53	theme	strain	1323:1328	arg1	M8JJ-5T					1330:1336	strain M8JJ-5T	1323:1336	strain M8JJ-5T	1323:1336	On the basis of phylogenetic, chemotaxonomic and phenotypic data, strain M8JJ-5T can be characterized to represent a novel species of the genus Amnibacterium, for which the name Amnibacteriumflavum sp.
30511915	9	54	theme	predominant	1014:1024	arg1	lipids					1032:1037	The predominant polar lipids	1010:1037	The predominant polar lipids	1010:1037	The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, an unidentified glycolipid, an unidentified phospholipid and an unidentified lipid, while the major fatty acids were iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0.
30511915	9	54	theme	predominant	1014:1024	arg1	diphosphatidylglycerol					1044:1065	diphosphatidylglycerol	1044:1065	diphosphatidylglycerol	1044:1065	The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, an unidentified glycolipid, an unidentified phospholipid and an unidentified lipid, while the major fatty acids were iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0.
30511915	1	55	theme	novel	33:37	arg1	actinobacterium					50:64	a novel endophytic actinobacterium	31:64	a novel endophytic actinobacterium isolated from bark of Nerium indicum Mill	31:106	nov., a novel endophytic actinobacterium isolated from bark of Nerium indicum Mill.
30511915	6	56	theme	Amnibacterium	806:818	arg1	KSL51201-037T					832:844	Amnibacterium kyonggiense KSL51201-037T	806:844	Amnibacterium kyonggiense KSL51201-037T	806:844	Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain M8JJ-5T was most closely related to the type strains of genus Amnibacterium, and shared highest 16S rRNA gene sequence similarity of 97.29 % to Amnibacterium kyonggiense KSL51201-037T.
30511915	2	57	theme	Neriumindicum	268:280	arg1	Mill					282:285	Neriumindicum Mill	268:285	Neriumindicum Mill	268:285	A Gram-stain-positive, aerobic, short-rod-shaped, non-spore-forming actinobacterial strain, designated M8JJ-5T, was isolated from a surface-sterilized bark of Neriumindicum Mill.
30511915	6	58	theme	Amnibacterium	724:736	arg1	strains					707:713	the type strains	698:713	the type strains of genus Amnibacterium	698:736	Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain M8JJ-5T was most closely related to the type strains of genus Amnibacterium, and shared highest 16S rRNA gene sequence similarity of 97.29 % to Amnibacterium kyonggiense KSL51201-037T.
30511915	9	59	theme	polar	1026:1030	arg1	lipids					1032:1037	The predominant polar lipids	1010:1037	The predominant polar lipids	1010:1037	The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, an unidentified glycolipid, an unidentified phospholipid and an unidentified lipid, while the major fatty acids were iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0.
30511915	9	59	theme	polar	1026:1030	arg1	diphosphatidylglycerol					1044:1065	diphosphatidylglycerol	1044:1065	diphosphatidylglycerol	1044:1065	The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, an unidentified glycolipid, an unidentified phospholipid and an unidentified lipid, while the major fatty acids were iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0.
30511915	8	60	theme	cell-wall	904:912	arg1	peptidoglycan					914:926	The cell-wall peptidoglycan	900:926	The cell-wall peptidoglycan	900:926	The cell-wall peptidoglycan contained l-2,4-diaminobutyric acid and MK-12, MK-11 were the major menaquinones.
30511915	1	61	dep	actinobacterium	50:64	arg1	nov.					25:28	nov.	25:28	nov.	25:28	nov., a novel endophytic actinobacterium isolated from bark of Nerium indicum Mill.
30511915	6	62	theme	%	801:801	arg1	similarity					781:790	highest 16S rRNA gene sequence similarity	750:790	highest 16S rRNA gene sequence similarity of 97.29 % to Amnibacterium kyonggiense KSL51201-037T	750:844	Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain M8JJ-5T was most closely related to the type strains of genus Amnibacterium, and shared highest 16S rRNA gene sequence similarity of 97.29 % to Amnibacterium kyonggiense KSL51201-037T.
30511915	7	63	theme	strain	870:875	arg1	M8JJ-5T					877:883	strain M8JJ-5T	870:883	strain M8JJ-5T	870:883	The DNA G+C content of strain M8JJ-5T was 68.6 mol%.
30511915	6	64	theme	gene	629:632	arg1	sequence					634:641	the 16S rRNA gene sequence	616:641	the 16S rRNA gene sequence	616:641	Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain M8JJ-5T was most closely related to the type strains of genus Amnibacterium, and shared highest 16S rRNA gene sequence similarity of 97.29 % to Amnibacterium kyonggiense KSL51201-037T.
30511915	2	65	theme	Gram-stain-positive	111:129	arg1	strain					193:198	A Gram-stain-positive, aerobic, short-rod-shaped, non-spore-forming actinobacterial strain	109:198	A Gram-stain-positive, aerobic, short-rod-shaped, non-spore-forming actinobacterial strain	109:198	A Gram-stain-positive, aerobic, short-rod-shaped, non-spore-forming actinobacterial strain, designated M8JJ-5T, was isolated from a surface-sterilized bark of Neriumindicum Mill.
30511915	2	66	theme	surface-sterilized	241:258	arg1	bark					260:263	a surface-sterilized bark	239:263	a surface-sterilized bark of Neriumindicum Mill	239:285	A Gram-stain-positive, aerobic, short-rod-shaped, non-spore-forming actinobacterial strain, designated M8JJ-5T, was isolated from a surface-sterilized bark of Neriumindicum Mill.
30511915	9	67	theme	major	1184:1188	arg1	iso-C16 					1207:1214	iso-C16 	1207:1214	iso-C16 	1207:1214	The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, an unidentified glycolipid, an unidentified phospholipid and an unidentified lipid, while the major fatty acids were iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0.
30511915	9	67	theme	major	1184:1188	arg1	acids					1196:1200	the major fatty acids	1180:1200	the major fatty acids	1180:1200	The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, an unidentified glycolipid, an unidentified phospholipid and an unidentified lipid, while the major fatty acids were iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0.
30511915	6	68	theme	rRNA	624:627	arg1	sequence					634:641	the 16S rRNA gene sequence	616:641	the 16S rRNA gene sequence	616:641	Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain M8JJ-5T was most closely related to the type strains of genus Amnibacterium, and shared highest 16S rRNA gene sequence similarity of 97.29 % to Amnibacterium kyonggiense KSL51201-037T.
30184260	6	0	theme	immune	1253:1258	arg1	response					1260:1267	the innate immune response	1242:1267	the innate immune response	1242:1267	KEY RESULTS Novel immunomodulatory activity of tetracyclines was identifed; they potentiated the innate immune response and enhanced resolution of inflammation.
30184260	10	1	theme	immune	1905:1910	arg1	responses					1912:1920	type 2 intestinal immune responses	1887:1920	type 2 intestinal immune responses	1887:1920	In particular, minocycline initially enhanced IL-1β, IL-6, IL-22, GM-CSF and IL-4 colonic production and monocyte recruitment to the intestine, subsequently increasing Ly6C- MHCII+ macrophages, Tregs and type 2 intestinal immune responses.
30184260	4	2	dep	APPROACH	556:563	arg1	study					595:599	A comparative pharmacological study	565:599	EXPERIMENTAL APPROACH A comparative pharmacological study	543:599	EXPERIMENTAL APPROACH A comparative pharmacological study was initially used to elucidate the most relevant actions of immunomodulatory tetracyclines: doxycycline, minocycline and tigecycline; other antibiotic or immunomodulatory drugs were assessed in bone marrow-derived macrophages and in dextran sodium sulfate (DSS)-induced mouse colitis, where different barrier markers, inflammatory mediators, microRNAs, TLRs, and the gut microbiota composition were evaluated.
30184260	11	3	dep	CONCLUSIONS	1923:1933	arg1	tetracyclines					1969:1981	Immunomodulatory tetracyclines	1952:1981	Immunomodulatory tetracyclines	1952:1981	CONCLUSIONS AND IMPLICATIONS Immunomodulatory tetracyclines potentiate protective immune pathways leading to mucosal healing and resolution, representing a promising drug reposition strategy for the treatment of intestinal inflammation.
30184260	8	4	theme	therapeutic	1415:1425	arg1	benefit					1427:1433	A minor therapeutic benefit	1407:1433	A minor therapeutic benefit	1407:1433	A minor therapeutic benefit seems to derive from their antibiotic properties.
30184260	2	5	theme	experimental	342:353	arg1	colitis					355:361	experimental colitis	342:361	experimental colitis	342:361	Specifically, minocycline and doxycycline have shown beneficial effects in experimental colitis, although pro-inflammatory actions have also been described in macrophages.
30184260	4	6	theme	gut	969:971	arg1	composition					984:994	the gut microbiota composition	965:994	the gut microbiota composition	965:994	EXPERIMENTAL APPROACH A comparative pharmacological study was initially used to elucidate the most relevant actions of immunomodulatory tetracyclines: doxycycline, minocycline and tigecycline; other antibiotic or immunomodulatory drugs were assessed in bone marrow-derived macrophages and in dextran sodium sulfate (DSS)-induced mouse colitis, where different barrier markers, inflammatory mediators, microRNAs, TLRs, and the gut microbiota composition were evaluated.
30184260	4	7	theme	marrow-derived	801:814	arg1	macrophages					816:826	bone marrow-derived macrophages	796:826	bone marrow-derived macrophages	796:826	EXPERIMENTAL APPROACH A comparative pharmacological study was initially used to elucidate the most relevant actions of immunomodulatory tetracyclines: doxycycline, minocycline and tigecycline; other antibiotic or immunomodulatory drugs were assessed in bone marrow-derived macrophages and in dextran sodium sulfate (DSS)-induced mouse colitis, where different barrier markers, inflammatory mediators, microRNAs, TLRs, and the gut microbiota composition were evaluated.
30184260	11	8	theme	mucosal	2032:2038	arg1	healing					2040:2046	mucosal healing	2032:2046	mucosal healing	2032:2046	CONCLUSIONS AND IMPLICATIONS Immunomodulatory tetracyclines potentiate protective immune pathways leading to mucosal healing and resolution, representing a promising drug reposition strategy for the treatment of intestinal inflammation.
30184260	1	9	theme	antibiotic	244:253	arg1	properties					255:264	their antibiotic properties	238:264	their antibiotic properties	238:264	BACKGROUND AND PURPOSE Immunomodulatory tetracyclines are well-characterized drugs with a pharmacological potential beyond their antibiotic properties.
30184260	4	10	theme	immunomodulatory	756:771	arg1	drugs					773:777	other antibiotic or immunomodulatory drugs	736:777	other antibiotic or immunomodulatory drugs	736:777	EXPERIMENTAL APPROACH A comparative pharmacological study was initially used to elucidate the most relevant actions of immunomodulatory tetracyclines: doxycycline, minocycline and tigecycline; other antibiotic or immunomodulatory drugs were assessed in bone marrow-derived macrophages and in dextran sodium sulfate (DSS)-induced mouse colitis, where different barrier markers, inflammatory mediators, microRNAs, TLRs, and the gut microbiota composition were evaluated.
30184260	5	11	theme	anti-inflammatory	1069:1085	arg1	effect					1087:1092	the intestinal anti-inflammatory effect	1054:1092	the intestinal anti-inflammatory effect of minocycline in DSS-colitis	1054:1122	The sequential immune events that mediate the intestinal anti-inflammatory effect of minocycline in DSS-colitis were then characterized.
30184260	2	12	theme	pro-inflammatory	373:388	arg1	actions					390:396	pro-inflammatory actions	373:396	pro-inflammatory actions	373:396	Specifically, minocycline and doxycycline have shown beneficial effects in experimental colitis, although pro-inflammatory actions have also been described in macrophages.
30184260	4	13	link	marrow-derived	801:814	arg1	macrophages					816:826	bone marrow-derived macrophages	796:826	bone marrow-derived macrophages	796:826	EXPERIMENTAL APPROACH A comparative pharmacological study was initially used to elucidate the most relevant actions of immunomodulatory tetracyclines: doxycycline, minocycline and tigecycline; other antibiotic or immunomodulatory drugs were assessed in bone marrow-derived macrophages and in dextran sodium sulfate (DSS)-induced mouse colitis, where different barrier markers, inflammatory mediators, microRNAs, TLRs, and the gut microbiota composition were evaluated.
30184260	11	14	theme	intestinal	2135:2144	arg1	inflammation					2146:2157	intestinal inflammation	2135:2157	intestinal inflammation	2135:2157	CONCLUSIONS AND IMPLICATIONS Immunomodulatory tetracyclines potentiate protective immune pathways leading to mucosal healing and resolution, representing a promising drug reposition strategy for the treatment of intestinal inflammation.
30184260	11	15	theme	Immunomodulatory	1952:1967	arg1	tetracyclines					1969:1981	Immunomodulatory tetracyclines	1952:1981	Immunomodulatory tetracyclines	1952:1981	CONCLUSIONS AND IMPLICATIONS Immunomodulatory tetracyclines potentiate protective immune pathways leading to mucosal healing and resolution, representing a promising drug reposition strategy for the treatment of intestinal inflammation.
30184260	1	16	theme	well-characterized	173:190	arg1	tetracyclines					155:167	BACKGROUND AND PURPOSE Immunomodulatory tetracyclines	115:167	BACKGROUND AND PURPOSE Immunomodulatory tetracyclines	115:167	BACKGROUND AND PURPOSE Immunomodulatory tetracyclines are well-characterized drugs with a pharmacological potential beyond their antibiotic properties.
30184260	1	16	theme	well-characterized	173:190	arg1	drugs					192:196	well-characterized drugs	173:196	well-characterized drugs with a pharmacological potential beyond their antibiotic properties	173:264	BACKGROUND AND PURPOSE Immunomodulatory tetracyclines are well-characterized drugs with a pharmacological potential beyond their antibiotic properties.
30184260	6	17	theme	immunomodulatory	1167:1182	arg1	activity					1184:1191	KEY RESULTS Novel immunomodulatory activity	1149:1191	KEY RESULTS Novel immunomodulatory activity of tetracyclines	1149:1208	KEY RESULTS Novel immunomodulatory activity of tetracyclines was identifed; they potentiated the innate immune response and enhanced resolution of inflammation.
30184260	11	18	theme	inflammation	2146:2157	arg1	treatment					2122:2130	the treatment	2118:2130	the treatment of intestinal inflammation	2118:2157	CONCLUSIONS AND IMPLICATIONS Immunomodulatory tetracyclines potentiate protective immune pathways leading to mucosal healing and resolution, representing a promising drug reposition strategy for the treatment of intestinal inflammation.
30184260	0	19	theme	mucosal	83:89	arg1	healing					91:97	mucosal healing	83:97	mucosal healing	83:97	Immunomodulatory tetracyclines shape the intestinal inflammatory response inducing mucosal healing and resolution.
30184260	7	20	theme	first	1327:1331	arg1	report					1333:1338	the first report	1323:1338	the first report describing the intestinal anti-inflammatory effect of tigecycline	1323:1404	This is also the first report describing the intestinal anti-inflammatory effect of tigecycline.
30184260	7	20	theme	first	1327:1331	arg1	This					1310:1313	This	1310:1313	This	1310:1313	This is also the first report describing the intestinal anti-inflammatory effect of tigecycline.
30184260	6	21	theme	RESULTS	1153:1159	arg1	activity					1184:1191	KEY RESULTS Novel immunomodulatory activity	1149:1191	KEY RESULTS Novel immunomodulatory activity of tetracyclines	1149:1208	KEY RESULTS Novel immunomodulatory activity of tetracyclines was identifed; they potentiated the innate immune response and enhanced resolution of inflammation.
30184260	11	22	theme	immune	2005:2010	arg1	pathways					2012:2019	protective immune pathways	1994:2019	protective immune pathways leading to mucosal healing and resolution	1994:2061	CONCLUSIONS AND IMPLICATIONS Immunomodulatory tetracyclines potentiate protective immune pathways leading to mucosal healing and resolution, representing a promising drug reposition strategy for the treatment of intestinal inflammation.
30184260	7	23	theme	tigecycline	1394:1404	arg1	effect					1384:1389	the intestinal anti-inflammatory effect	1351:1389	the intestinal anti-inflammatory effect of tigecycline	1351:1404	This is also the first report describing the intestinal anti-inflammatory effect of tigecycline.
30184260	1	24	theme	pharmacological	205:219	arg1	potential					221:229	a pharmacological potential	203:229	a pharmacological potential beyond their antibiotic properties	203:264	BACKGROUND AND PURPOSE Immunomodulatory tetracyclines are well-characterized drugs with a pharmacological potential beyond their antibiotic properties.
30184260	4	25	theme	comparative	567:577	arg1	study					595:599	A comparative pharmacological study	565:599	EXPERIMENTAL APPROACH A comparative pharmacological study	543:599	EXPERIMENTAL APPROACH A comparative pharmacological study was initially used to elucidate the most relevant actions of immunomodulatory tetracyclines: doxycycline, minocycline and tigecycline; other antibiotic or immunomodulatory drugs were assessed in bone marrow-derived macrophages and in dextran sodium sulfate (DSS)-induced mouse colitis, where different barrier markers, inflammatory mediators, microRNAs, TLRs, and the gut microbiota composition were evaluated.
30184260	3	26	dep	mechanism	479:487	arg1	behind					489:494	behind	489:494	behind their effect in acute intestinal inflammation	489:540	Therefore, we aimed to characterize the mechanism behind their effect in acute intestinal inflammation.
30184260	4	27	theme	barrier	903:909	arg1	microRNAs					944:952	microRNAs	944:952	microRNAs	944:952	EXPERIMENTAL APPROACH A comparative pharmacological study was initially used to elucidate the most relevant actions of immunomodulatory tetracyclines: doxycycline, minocycline and tigecycline; other antibiotic or immunomodulatory drugs were assessed in bone marrow-derived macrophages and in dextran sodium sulfate (DSS)-induced mouse colitis, where different barrier markers, inflammatory mediators, microRNAs, TLRs, and the gut microbiota composition were evaluated.
30184260	4	27	theme	barrier	903:909	arg1	markers					911:917	different barrier markers	893:917	different barrier markers	893:917	EXPERIMENTAL APPROACH A comparative pharmacological study was initially used to elucidate the most relevant actions of immunomodulatory tetracyclines: doxycycline, minocycline and tigecycline; other antibiotic or immunomodulatory drugs were assessed in bone marrow-derived macrophages and in dextran sodium sulfate (DSS)-induced mouse colitis, where different barrier markers, inflammatory mediators, microRNAs, TLRs, and the gut microbiota composition were evaluated.
30184260	4	27	theme	barrier	903:909	arg1	mediators					933:941	inflammatory mediators	920:941	inflammatory mediators	920:941	EXPERIMENTAL APPROACH A comparative pharmacological study was initially used to elucidate the most relevant actions of immunomodulatory tetracyclines: doxycycline, minocycline and tigecycline; other antibiotic or immunomodulatory drugs were assessed in bone marrow-derived macrophages and in dextran sodium sulfate (DSS)-induced mouse colitis, where different barrier markers, inflammatory mediators, microRNAs, TLRs, and the gut microbiota composition were evaluated.
30184260	4	27	theme	barrier	903:909	arg1	TLRs					955:958	TLRs	955:958	TLRs	955:958	EXPERIMENTAL APPROACH A comparative pharmacological study was initially used to elucidate the most relevant actions of immunomodulatory tetracyclines: doxycycline, minocycline and tigecycline; other antibiotic or immunomodulatory drugs were assessed in bone marrow-derived macrophages and in dextran sodium sulfate (DSS)-induced mouse colitis, where different barrier markers, inflammatory mediators, microRNAs, TLRs, and the gut microbiota composition were evaluated.
30184260	10	28	theme	Ly6C-	1851:1855	arg1	macrophages					1864:1874	Ly6C- MHCII+ macrophages	1851:1874	Ly6C- MHCII+ macrophages	1851:1874	In particular, minocycline initially enhanced IL-1β, IL-6, IL-22, GM-CSF and IL-4 colonic production and monocyte recruitment to the intestine, subsequently increasing Ly6C- MHCII+ macrophages, Tregs and type 2 intestinal immune responses.
30184260	7	29	theme	intestinal	1355:1364	arg1	effect					1384:1389	the intestinal anti-inflammatory effect	1351:1389	the intestinal anti-inflammatory effect of tigecycline	1351:1404	This is also the first report describing the intestinal anti-inflammatory effect of tigecycline.
30184260	4	30	theme	-induced	863:870	arg1	colitis					878:884	dextran sodium sulfate (DSS)-induced mouse colitis	835:884	dextran sodium sulfate (DSS)-induced mouse colitis	835:884	EXPERIMENTAL APPROACH A comparative pharmacological study was initially used to elucidate the most relevant actions of immunomodulatory tetracyclines: doxycycline, minocycline and tigecycline; other antibiotic or immunomodulatory drugs were assessed in bone marrow-derived macrophages and in dextran sodium sulfate (DSS)-induced mouse colitis, where different barrier markers, inflammatory mediators, microRNAs, TLRs, and the gut microbiota composition were evaluated.
30184260	0	31	theme	Immunomodulatory	0:15	arg1	tetracyclines					17:29	Immunomodulatory tetracyclines	0:29	Immunomodulatory tetracyclines	0:29	Immunomodulatory tetracyclines shape the intestinal inflammatory response inducing mucosal healing and resolution.
30184260	9	32	theme	macrophage	1540:1549	arg1	release					1560:1566	macrophage cytokine release	1540:1566	macrophage cytokine release	1540:1566	Conversely, immunomodulatory tetracyclines potentiated macrophage cytokine release in vitro, and while improving mucosal recovery in colitic mice, they up-regulated Ccl2, miR-142, miR-375 and Tlr4.
30184260	1	33	theme	BACKGROUND	115:124	arg1	tetracyclines					155:167	BACKGROUND AND PURPOSE Immunomodulatory tetracyclines	115:167	BACKGROUND AND PURPOSE Immunomodulatory tetracyclines	115:167	BACKGROUND AND PURPOSE Immunomodulatory tetracyclines are well-characterized drugs with a pharmacological potential beyond their antibiotic properties.
30184260	1	33	theme	BACKGROUND	115:124	arg1	drugs					192:196	well-characterized drugs	173:196	well-characterized drugs with a pharmacological potential beyond their antibiotic properties	173:264	BACKGROUND AND PURPOSE Immunomodulatory tetracyclines are well-characterized drugs with a pharmacological potential beyond their antibiotic properties.
30184260	4	34	used	used	615:618	arg2	APPROACH					556:563	EXPERIMENTAL APPROACH	543:563	EXPERIMENTAL APPROACH A comparative pharmacological study	543:599	EXPERIMENTAL APPROACH A comparative pharmacological study was initially used to elucidate the most relevant actions of immunomodulatory tetracyclines: doxycycline, minocycline and tigecycline; other antibiotic or immunomodulatory drugs were assessed in bone marrow-derived macrophages and in dextran sodium sulfate (DSS)-induced mouse colitis, where different barrier markers, inflammatory mediators, microRNAs, TLRs, and the gut microbiota composition were evaluated.
30184260	1	35	theme	PURPOSE	130:136	arg1	tetracyclines					155:167	BACKGROUND AND PURPOSE Immunomodulatory tetracyclines	115:167	BACKGROUND AND PURPOSE Immunomodulatory tetracyclines	115:167	BACKGROUND AND PURPOSE Immunomodulatory tetracyclines are well-characterized drugs with a pharmacological potential beyond their antibiotic properties.
30184260	1	35	theme	PURPOSE	130:136	arg1	drugs					192:196	well-characterized drugs	173:196	well-characterized drugs with a pharmacological potential beyond their antibiotic properties	173:264	BACKGROUND AND PURPOSE Immunomodulatory tetracyclines are well-characterized drugs with a pharmacological potential beyond their antibiotic properties.
30184260	4	36	theme	tetracyclines	679:691	arg1	actions					651:657	the most relevant actions	633:657	the most relevant actions of immunomodulatory tetracyclines	633:691	EXPERIMENTAL APPROACH A comparative pharmacological study was initially used to elucidate the most relevant actions of immunomodulatory tetracyclines: doxycycline, minocycline and tigecycline; other antibiotic or immunomodulatory drugs were assessed in bone marrow-derived macrophages and in dextran sodium sulfate (DSS)-induced mouse colitis, where different barrier markers, inflammatory mediators, microRNAs, TLRs, and the gut microbiota composition were evaluated.
30184260	0	37	theme	intestinal	41:50	arg1	response					65:72	the intestinal inflammatory response	37:72	the intestinal inflammatory response inducing mucosal healing and resolution	37:112	Immunomodulatory tetracyclines shape the intestinal inflammatory response inducing mucosal healing and resolution.
30184260	10	38	theme	IL-1β	1729:1733	arg1	production					1773:1782	IL-1β, IL-6, IL-22, GM-CSF and IL-4 colonic production	1729:1782	production	1773:1782	In particular, minocycline initially enhanced IL-1β, IL-6, IL-22, GM-CSF and IL-4 colonic production and monocyte recruitment to the intestine, subsequently increasing Ly6C- MHCII+ macrophages, Tregs and type 2 intestinal immune responses.
30184260	11	39	theme	drug	2089:2092	arg1	strategy					2105:2112	a promising drug reposition strategy	2077:2112	a promising drug reposition strategy for the treatment of intestinal inflammation	2077:2157	CONCLUSIONS AND IMPLICATIONS Immunomodulatory tetracyclines potentiate protective immune pathways leading to mucosal healing and resolution, representing a promising drug reposition strategy for the treatment of intestinal inflammation.
30184260	4	40	dep	used	615:618	arg1	minocycline					707:717	minocycline	707:717	minocycline	707:717	EXPERIMENTAL APPROACH A comparative pharmacological study was initially used to elucidate the most relevant actions of immunomodulatory tetracyclines: doxycycline, minocycline and tigecycline; other antibiotic or immunomodulatory drugs were assessed in bone marrow-derived macrophages and in dextran sodium sulfate (DSS)-induced mouse colitis, where different barrier markers, inflammatory mediators, microRNAs, TLRs, and the gut microbiota composition were evaluated.
30184260	4	40	dep	used	615:618	arg1	doxycycline					694:704	doxycycline	694:704	doxycycline	694:704	EXPERIMENTAL APPROACH A comparative pharmacological study was initially used to elucidate the most relevant actions of immunomodulatory tetracyclines: doxycycline, minocycline and tigecycline; other antibiotic or immunomodulatory drugs were assessed in bone marrow-derived macrophages and in dextran sodium sulfate (DSS)-induced mouse colitis, where different barrier markers, inflammatory mediators, microRNAs, TLRs, and the gut microbiota composition were evaluated.
30184260	4	40	dep	used	615:618	arg1	tigecycline					723:733	tigecycline	723:733	tigecycline	723:733	EXPERIMENTAL APPROACH A comparative pharmacological study was initially used to elucidate the most relevant actions of immunomodulatory tetracyclines: doxycycline, minocycline and tigecycline; other antibiotic or immunomodulatory drugs were assessed in bone marrow-derived macrophages and in dextran sodium sulfate (DSS)-induced mouse colitis, where different barrier markers, inflammatory mediators, microRNAs, TLRs, and the gut microbiota composition were evaluated.
30184260	4	41	theme	relevant	642:649	arg1	actions					651:657	the most relevant actions	633:657	the most relevant actions of immunomodulatory tetracyclines	633:691	EXPERIMENTAL APPROACH A comparative pharmacological study was initially used to elucidate the most relevant actions of immunomodulatory tetracyclines: doxycycline, minocycline and tigecycline; other antibiotic or immunomodulatory drugs were assessed in bone marrow-derived macrophages and in dextran sodium sulfate (DSS)-induced mouse colitis, where different barrier markers, inflammatory mediators, microRNAs, TLRs, and the gut microbiota composition were evaluated.
30184260	10	42	theme	type	1887:1890	arg1	responses					1912:1920	type 2 intestinal immune responses	1887:1920	type 2 intestinal immune responses	1887:1920	In particular, minocycline initially enhanced IL-1β, IL-6, IL-22, GM-CSF and IL-4 colonic production and monocyte recruitment to the intestine, subsequently increasing Ly6C- MHCII+ macrophages, Tregs and type 2 intestinal immune responses.
30184260	10	43	theme	IL-22	1742:1746	arg1	production					1773:1782	IL-1β, IL-6, IL-22, GM-CSF and IL-4 colonic production	1729:1782	production	1773:1782	In particular, minocycline initially enhanced IL-1β, IL-6, IL-22, GM-CSF and IL-4 colonic production and monocyte recruitment to the intestine, subsequently increasing Ly6C- MHCII+ macrophages, Tregs and type 2 intestinal immune responses.
30184260	4	44	theme	other	736:740	arg1	drugs					773:777	other antibiotic or immunomodulatory drugs	736:777	other antibiotic or immunomodulatory drugs	736:777	EXPERIMENTAL APPROACH A comparative pharmacological study was initially used to elucidate the most relevant actions of immunomodulatory tetracyclines: doxycycline, minocycline and tigecycline; other antibiotic or immunomodulatory drugs were assessed in bone marrow-derived macrophages and in dextran sodium sulfate (DSS)-induced mouse colitis, where different barrier markers, inflammatory mediators, microRNAs, TLRs, and the gut microbiota composition were evaluated.
30184260	10	45	theme	IL-6	1736:1739	arg1	production					1773:1782	IL-1β, IL-6, IL-22, GM-CSF and IL-4 colonic production	1729:1782	production	1773:1782	In particular, minocycline initially enhanced IL-1β, IL-6, IL-22, GM-CSF and IL-4 colonic production and monocyte recruitment to the intestine, subsequently increasing Ly6C- MHCII+ macrophages, Tregs and type 2 intestinal immune responses.
30184260	9	46	theme	colitic	1618:1624	arg1	mice					1626:1629	colitic mice	1618:1629	colitic mice	1618:1629	Conversely, immunomodulatory tetracyclines potentiated macrophage cytokine release in vitro, and while improving mucosal recovery in colitic mice, they up-regulated Ccl2, miR-142, miR-375 and Tlr4.
30184260	4	47	theme	microbiota	973:982	arg1	composition					984:994	the gut microbiota composition	965:994	the gut microbiota composition	965:994	EXPERIMENTAL APPROACH A comparative pharmacological study was initially used to elucidate the most relevant actions of immunomodulatory tetracyclines: doxycycline, minocycline and tigecycline; other antibiotic or immunomodulatory drugs were assessed in bone marrow-derived macrophages and in dextran sodium sulfate (DSS)-induced mouse colitis, where different barrier markers, inflammatory mediators, microRNAs, TLRs, and the gut microbiota composition were evaluated.
30184260	6	48	theme	innate	1246:1251	arg1	response					1260:1267	the innate immune response	1242:1267	the innate immune response	1242:1267	KEY RESULTS Novel immunomodulatory activity of tetracyclines was identifed; they potentiated the innate immune response and enhanced resolution of inflammation.
30184260	10	49	theme	IL-4	1760:1763	arg1	production					1773:1782	IL-1β, IL-6, IL-22, GM-CSF and IL-4 colonic production	1729:1782	production	1773:1782	In particular, minocycline initially enhanced IL-1β, IL-6, IL-22, GM-CSF and IL-4 colonic production and monocyte recruitment to the intestine, subsequently increasing Ly6C- MHCII+ macrophages, Tregs and type 2 intestinal immune responses.
30184260	10	50	theme	GM-CSF	1749:1754	arg1	production					1773:1782	IL-1β, IL-6, IL-22, GM-CSF and IL-4 colonic production	1729:1782	production	1773:1782	In particular, minocycline initially enhanced IL-1β, IL-6, IL-22, GM-CSF and IL-4 colonic production and monocyte recruitment to the intestine, subsequently increasing Ly6C- MHCII+ macrophages, Tregs and type 2 intestinal immune responses.
30184260	10	51	theme	intestinal	1894:1903	arg1	responses					1912:1920	type 2 intestinal immune responses	1887:1920	type 2 intestinal immune responses	1887:1920	In particular, minocycline initially enhanced IL-1β, IL-6, IL-22, GM-CSF and IL-4 colonic production and monocyte recruitment to the intestine, subsequently increasing Ly6C- MHCII+ macrophages, Tregs and type 2 intestinal immune responses.
30184260	8	52	theme	minor	1409:1413	arg1	benefit					1427:1433	A minor therapeutic benefit	1407:1433	A minor therapeutic benefit	1407:1433	A minor therapeutic benefit seems to derive from their antibiotic properties.
30184260	10	53	theme	monocyte	1788:1795	arg1	recruitment					1797:1807	monocyte recruitment	1788:1807	monocyte recruitment to the intestine	1788:1824	In particular, minocycline initially enhanced IL-1β, IL-6, IL-22, GM-CSF and IL-4 colonic production and monocyte recruitment to the intestine, subsequently increasing Ly6C- MHCII+ macrophages, Tregs and type 2 intestinal immune responses.
30184260	4	54	theme	bone	796:799	arg1	macrophages					816:826	bone marrow-derived macrophages	796:826	bone marrow-derived macrophages	796:826	EXPERIMENTAL APPROACH A comparative pharmacological study was initially used to elucidate the most relevant actions of immunomodulatory tetracyclines: doxycycline, minocycline and tigecycline; other antibiotic or immunomodulatory drugs were assessed in bone marrow-derived macrophages and in dextran sodium sulfate (DSS)-induced mouse colitis, where different barrier markers, inflammatory mediators, microRNAs, TLRs, and the gut microbiota composition were evaluated.
30184260	4	55	theme	EXPERIMENTAL	543:554	arg1	APPROACH					556:563	EXPERIMENTAL APPROACH	543:563	EXPERIMENTAL APPROACH A comparative pharmacological study	543:599	EXPERIMENTAL APPROACH A comparative pharmacological study was initially used to elucidate the most relevant actions of immunomodulatory tetracyclines: doxycycline, minocycline and tigecycline; other antibiotic or immunomodulatory drugs were assessed in bone marrow-derived macrophages and in dextran sodium sulfate (DSS)-induced mouse colitis, where different barrier markers, inflammatory mediators, microRNAs, TLRs, and the gut microbiota composition were evaluated.
30184260	5	56	theme	immune	1027:1032	arg1	events					1034:1039	The sequential immune events	1012:1039	The sequential immune events that mediate the intestinal anti-inflammatory effect of minocycline in DSS-colitis	1012:1122	The sequential immune events that mediate the intestinal anti-inflammatory effect of minocycline in DSS-colitis were then characterized.
30184260	1	57	theme	Immunomodulatory	138:153	arg1	tetracyclines					155:167	BACKGROUND AND PURPOSE Immunomodulatory tetracyclines	115:167	BACKGROUND AND PURPOSE Immunomodulatory tetracyclines	115:167	BACKGROUND AND PURPOSE Immunomodulatory tetracyclines are well-characterized drugs with a pharmacological potential beyond their antibiotic properties.
30184260	1	57	theme	Immunomodulatory	138:153	arg1	drugs					192:196	well-characterized drugs	173:196	well-characterized drugs with a pharmacological potential beyond their antibiotic properties	173:264	BACKGROUND AND PURPOSE Immunomodulatory tetracyclines are well-characterized drugs with a pharmacological potential beyond their antibiotic properties.
30184260	8	58	theme	antibiotic	1462:1471	arg1	properties					1473:1482	their antibiotic properties	1456:1482	their antibiotic properties	1456:1482	A minor therapeutic benefit seems to derive from their antibiotic properties.
30184260	2	59	theme	beneficial	320:329	arg1	effects					331:337	beneficial effects	320:337	beneficial effects in experimental colitis	320:361	Specifically, minocycline and doxycycline have shown beneficial effects in experimental colitis, although pro-inflammatory actions have also been described in macrophages.
30184260	6	60	theme	Novel	1161:1165	arg1	activity					1184:1191	KEY RESULTS Novel immunomodulatory activity	1149:1191	KEY RESULTS Novel immunomodulatory activity of tetracyclines	1149:1208	KEY RESULTS Novel immunomodulatory activity of tetracyclines was identifed; they potentiated the innate immune response and enhanced resolution of inflammation.
30184260	10	61	theme	colonic	1765:1771	arg1	production					1773:1782	IL-1β, IL-6, IL-22, GM-CSF and IL-4 colonic production	1729:1782	production	1773:1782	In particular, minocycline initially enhanced IL-1β, IL-6, IL-22, GM-CSF and IL-4 colonic production and monocyte recruitment to the intestine, subsequently increasing Ly6C- MHCII+ macrophages, Tregs and type 2 intestinal immune responses.
30184260	5	62	theme	sequential	1016:1025	arg1	events					1034:1039	The sequential immune events	1012:1039	The sequential immune events that mediate the intestinal anti-inflammatory effect of minocycline in DSS-colitis	1012:1122	The sequential immune events that mediate the intestinal anti-inflammatory effect of minocycline in DSS-colitis were then characterized.
30184260	6	63	theme	KEY	1149:1151	arg1	RESULTS					1153:1159	KEY RESULTS	1149:1159	KEY RESULTS Novel immunomodulatory activity of tetracyclines	1149:1208	KEY RESULTS Novel immunomodulatory activity of tetracyclines was identifed; they potentiated the innate immune response and enhanced resolution of inflammation.
30184260	7	64	theme	anti-inflammatory	1366:1382	arg1	effect					1384:1389	the intestinal anti-inflammatory effect	1351:1389	the intestinal anti-inflammatory effect of tigecycline	1351:1404	This is also the first report describing the intestinal anti-inflammatory effect of tigecycline.
30184260	1	65	with	drugs	192:196	arg1	potential					221:229	a pharmacological potential	203:229	a pharmacological potential beyond their antibiotic properties	203:264	BACKGROUND AND PURPOSE Immunomodulatory tetracyclines are well-characterized drugs with a pharmacological potential beyond their antibiotic properties.
30184260	5	66	theme	minocycline	1097:1107	arg1	effect					1087:1092	the intestinal anti-inflammatory effect	1054:1092	the intestinal anti-inflammatory effect of minocycline in DSS-colitis	1054:1122	The sequential immune events that mediate the intestinal anti-inflammatory effect of minocycline in DSS-colitis were then characterized.
30184260	4	67	theme	pharmacological	579:593	arg1	study					595:599	A comparative pharmacological study	565:599	EXPERIMENTAL APPROACH A comparative pharmacological study	543:599	EXPERIMENTAL APPROACH A comparative pharmacological study was initially used to elucidate the most relevant actions of immunomodulatory tetracyclines: doxycycline, minocycline and tigecycline; other antibiotic or immunomodulatory drugs were assessed in bone marrow-derived macrophages and in dextran sodium sulfate (DSS)-induced mouse colitis, where different barrier markers, inflammatory mediators, microRNAs, TLRs, and the gut microbiota composition were evaluated.
30184260	11	68	theme	protective	1994:2003	arg1	pathways					2012:2019	protective immune pathways	1994:2019	protective immune pathways leading to mucosal healing and resolution	1994:2061	CONCLUSIONS AND IMPLICATIONS Immunomodulatory tetracyclines potentiate protective immune pathways leading to mucosal healing and resolution, representing a promising drug reposition strategy for the treatment of intestinal inflammation.
30184260	9	69	theme	immunomodulatory	1497:1512	arg1	tetracyclines					1514:1526	immunomodulatory tetracyclines	1497:1526	immunomodulatory tetracyclines	1497:1526	Conversely, immunomodulatory tetracyclines potentiated macrophage cytokine release in vitro, and while improving mucosal recovery in colitic mice, they up-regulated Ccl2, miR-142, miR-375 and Tlr4.
30184260	4	70	theme	different	893:901	arg1	microRNAs					944:952	microRNAs	944:952	microRNAs	944:952	EXPERIMENTAL APPROACH A comparative pharmacological study was initially used to elucidate the most relevant actions of immunomodulatory tetracyclines: doxycycline, minocycline and tigecycline; other antibiotic or immunomodulatory drugs were assessed in bone marrow-derived macrophages and in dextran sodium sulfate (DSS)-induced mouse colitis, where different barrier markers, inflammatory mediators, microRNAs, TLRs, and the gut microbiota composition were evaluated.
30184260	4	70	theme	different	893:901	arg1	markers					911:917	different barrier markers	893:917	different barrier markers	893:917	EXPERIMENTAL APPROACH A comparative pharmacological study was initially used to elucidate the most relevant actions of immunomodulatory tetracyclines: doxycycline, minocycline and tigecycline; other antibiotic or immunomodulatory drugs were assessed in bone marrow-derived macrophages and in dextran sodium sulfate (DSS)-induced mouse colitis, where different barrier markers, inflammatory mediators, microRNAs, TLRs, and the gut microbiota composition were evaluated.
30184260	4	70	theme	different	893:901	arg1	mediators					933:941	inflammatory mediators	920:941	inflammatory mediators	920:941	EXPERIMENTAL APPROACH A comparative pharmacological study was initially used to elucidate the most relevant actions of immunomodulatory tetracyclines: doxycycline, minocycline and tigecycline; other antibiotic or immunomodulatory drugs were assessed in bone marrow-derived macrophages and in dextran sodium sulfate (DSS)-induced mouse colitis, where different barrier markers, inflammatory mediators, microRNAs, TLRs, and the gut microbiota composition were evaluated.
30184260	4	70	theme	different	893:901	arg1	TLRs					955:958	TLRs	955:958	TLRs	955:958	EXPERIMENTAL APPROACH A comparative pharmacological study was initially used to elucidate the most relevant actions of immunomodulatory tetracyclines: doxycycline, minocycline and tigecycline; other antibiotic or immunomodulatory drugs were assessed in bone marrow-derived macrophages and in dextran sodium sulfate (DSS)-induced mouse colitis, where different barrier markers, inflammatory mediators, microRNAs, TLRs, and the gut microbiota composition were evaluated.
30184260	5	71	theme	intestinal	1058:1067	arg1	effect					1087:1092	the intestinal anti-inflammatory effect	1054:1092	the intestinal anti-inflammatory effect of minocycline in DSS-colitis	1054:1122	The sequential immune events that mediate the intestinal anti-inflammatory effect of minocycline in DSS-colitis were then characterized.
30184260	4	72	theme	immunomodulatory	662:677	arg1	tetracyclines					679:691	immunomodulatory tetracyclines	662:691	immunomodulatory tetracyclines	662:691	EXPERIMENTAL APPROACH A comparative pharmacological study was initially used to elucidate the most relevant actions of immunomodulatory tetracyclines: doxycycline, minocycline and tigecycline; other antibiotic or immunomodulatory drugs were assessed in bone marrow-derived macrophages and in dextran sodium sulfate (DSS)-induced mouse colitis, where different barrier markers, inflammatory mediators, microRNAs, TLRs, and the gut microbiota composition were evaluated.
30184260	3	73	theme	acute	512:516	arg1	inflammation					529:540	acute intestinal inflammation	512:540	acute intestinal inflammation	512:540	Therefore, we aimed to characterize the mechanism behind their effect in acute intestinal inflammation.
30184260	9	74	theme	cytokine	1551:1558	arg1	release					1560:1566	macrophage cytokine release	1540:1566	macrophage cytokine release	1540:1566	Conversely, immunomodulatory tetracyclines potentiated macrophage cytokine release in vitro, and while improving mucosal recovery in colitic mice, they up-regulated Ccl2, miR-142, miR-375 and Tlr4.
30184260	4	75	theme	mouse	872:876	arg1	colitis					878:884	dextran sodium sulfate (DSS)-induced mouse colitis	835:884	dextran sodium sulfate (DSS)-induced mouse colitis	835:884	EXPERIMENTAL APPROACH A comparative pharmacological study was initially used to elucidate the most relevant actions of immunomodulatory tetracyclines: doxycycline, minocycline and tigecycline; other antibiotic or immunomodulatory drugs were assessed in bone marrow-derived macrophages and in dextran sodium sulfate (DSS)-induced mouse colitis, where different barrier markers, inflammatory mediators, microRNAs, TLRs, and the gut microbiota composition were evaluated.
30184260	0	76	theme	inflammatory	52:63	arg1	response					65:72	the intestinal inflammatory response	37:72	the intestinal inflammatory response inducing mucosal healing and resolution	37:112	Immunomodulatory tetracyclines shape the intestinal inflammatory response inducing mucosal healing and resolution.
30184260	2	77	from	effects	331:337	arg1	colitis					355:361	experimental colitis	342:361	experimental colitis	342:361	Specifically, minocycline and doxycycline have shown beneficial effects in experimental colitis, although pro-inflammatory actions have also been described in macrophages.
30184260	6	78	theme	tetracyclines	1196:1208	arg1	activity					1184:1191	KEY RESULTS Novel immunomodulatory activity	1149:1191	KEY RESULTS Novel immunomodulatory activity of tetracyclines	1149:1208	KEY RESULTS Novel immunomodulatory activity of tetracyclines was identifed; they potentiated the innate immune response and enhanced resolution of inflammation.
30184260	3	79	theme	intestinal	518:527	arg1	inflammation					529:540	acute intestinal inflammation	512:540	acute intestinal inflammation	512:540	Therefore, we aimed to characterize the mechanism behind their effect in acute intestinal inflammation.
30184260	10	80	theme	MHCII+	1857:1862	arg1	macrophages					1864:1874	Ly6C- MHCII+ macrophages	1851:1874	Ly6C- MHCII+ macrophages	1851:1874	In particular, minocycline initially enhanced IL-1β, IL-6, IL-22, GM-CSF and IL-4 colonic production and monocyte recruitment to the intestine, subsequently increasing Ly6C- MHCII+ macrophages, Tregs and type 2 intestinal immune responses.
30184260	9	81	from	recovery	1606:1613	arg1	mice					1626:1629	colitic mice	1618:1629	colitic mice	1618:1629	Conversely, immunomodulatory tetracyclines potentiated macrophage cytokine release in vitro, and while improving mucosal recovery in colitic mice, they up-regulated Ccl2, miR-142, miR-375 and Tlr4.
30184260	6	82	theme	inflammation	1296:1307	arg1	resolution					1282:1291	resolution	1282:1291	resolution of inflammation	1282:1307	KEY RESULTS Novel immunomodulatory activity of tetracyclines was identifed; they potentiated the innate immune response and enhanced resolution of inflammation.
30184260	11	83	theme	reposition	2094:2103	arg1	strategy					2105:2112	a promising drug reposition strategy	2077:2112	a promising drug reposition strategy for the treatment of intestinal inflammation	2077:2157	CONCLUSIONS AND IMPLICATIONS Immunomodulatory tetracyclines potentiate protective immune pathways leading to mucosal healing and resolution, representing a promising drug reposition strategy for the treatment of intestinal inflammation.
30184260	5	84	from	effect	1087:1092	arg1	DSS-colitis					1112:1122	DSS-colitis	1112:1122	DSS-colitis	1112:1122	The sequential immune events that mediate the intestinal anti-inflammatory effect of minocycline in DSS-colitis were then characterized.
30184260	11	85	theme	promising	2079:2087	arg1	strategy					2105:2112	a promising drug reposition strategy	2077:2112	a promising drug reposition strategy for the treatment of intestinal inflammation	2077:2157	CONCLUSIONS AND IMPLICATIONS Immunomodulatory tetracyclines potentiate protective immune pathways leading to mucosal healing and resolution, representing a promising drug reposition strategy for the treatment of intestinal inflammation.
30184260	9	86	theme	mucosal	1598:1604	arg1	recovery					1606:1613	mucosal recovery	1598:1613	mucosal recovery in colitic mice	1598:1629	Conversely, immunomodulatory tetracyclines potentiated macrophage cytokine release in vitro, and while improving mucosal recovery in colitic mice, they up-regulated Ccl2, miR-142, miR-375 and Tlr4.
30184260	4	87	theme	antibiotic	742:751	arg1	drugs					773:777	other antibiotic or immunomodulatory drugs	736:777	other antibiotic or immunomodulatory drugs	736:777	EXPERIMENTAL APPROACH A comparative pharmacological study was initially used to elucidate the most relevant actions of immunomodulatory tetracyclines: doxycycline, minocycline and tigecycline; other antibiotic or immunomodulatory drugs were assessed in bone marrow-derived macrophages and in dextran sodium sulfate (DSS)-induced mouse colitis, where different barrier markers, inflammatory mediators, microRNAs, TLRs, and the gut microbiota composition were evaluated.
30184260	4	88	theme	inflammatory	920:931	arg1	markers					911:917	different barrier markers	893:917	different barrier markers	893:917	EXPERIMENTAL APPROACH A comparative pharmacological study was initially used to elucidate the most relevant actions of immunomodulatory tetracyclines: doxycycline, minocycline and tigecycline; other antibiotic or immunomodulatory drugs were assessed in bone marrow-derived macrophages and in dextran sodium sulfate (DSS)-induced mouse colitis, where different barrier markers, inflammatory mediators, microRNAs, TLRs, and the gut microbiota composition were evaluated.
30184260	4	88	theme	inflammatory	920:931	arg1	mediators					933:941	inflammatory mediators	920:941	inflammatory mediators	920:941	EXPERIMENTAL APPROACH A comparative pharmacological study was initially used to elucidate the most relevant actions of immunomodulatory tetracyclines: doxycycline, minocycline and tigecycline; other antibiotic or immunomodulatory drugs were assessed in bone marrow-derived macrophages and in dextran sodium sulfate (DSS)-induced mouse colitis, where different barrier markers, inflammatory mediators, microRNAs, TLRs, and the gut microbiota composition were evaluated.
31993173	3	0	theme	liquid-to-material	689:706	arg1	ratio					708:712	liquid-to-material ratio	689:712	liquid-to-material ratio of 41 ml/g	689:723	The optimal extraction technology was determined as extraction temperature of 83°C, time of 104 min, and liquid-to-material ratio of 41 ml/g, yielding TPP and TPS at 42.70 ± 2.38 mg/g and 53.86 ± 3.79 mg/g, respectively.
31993173	3	0	theme	liquid-to-material	689:706	arg1	technology					607:616	The optimal extraction technology	584:616	The optimal extraction technology	584:616	The optimal extraction technology was determined as extraction temperature of 83°C, time of 104 min, and liquid-to-material ratio of 41 ml/g, yielding TPP and TPS at 42.70 ± 2.38 mg/g and 53.86 ± 3.79 mg/g, respectively.
31993173	2	1	theme	hot	551:553	arg1	conditions					572:581	the hot water extraction conditions	547:581	the hot water extraction conditions	547:581	On the basis of single-factor experiments, a Box-Behnken design and response surface methodology were applied to optimize the hot water extraction conditions.
31993173	5	2	theme	polyphenol	1031:1040	arg1	GCG					1009:1011	GCG	1009:1011	GCG	1009:1011	UHPLC-QqQ-MS/MS analysis identified EGCG, GCG, and ECG as major polyphenol components in Ya'an Tibetan tea.
31993173	5	2	theme	polyphenol	1031:1040	arg1	ECG					1018:1020	ECG	1018:1020	ECG	1018:1020	UHPLC-QqQ-MS/MS analysis identified EGCG, GCG, and ECG as major polyphenol components in Ya'an Tibetan tea.
31993173	5	2	theme	polyphenol	1031:1040	arg1	EGCG					1003:1006	EGCG	1003:1006	EGCG	1003:1006	UHPLC-QqQ-MS/MS analysis identified EGCG, GCG, and ECG as major polyphenol components in Ya'an Tibetan tea.
31993173	5	2	theme	polyphenol	1031:1040	arg1	components					1042:1051	major polyphenol components	1025:1051	major polyphenol components in Ya'an Tibetan tea	1025:1072	UHPLC-QqQ-MS/MS analysis identified EGCG, GCG, and ECG as major polyphenol components in Ya'an Tibetan tea.
31993173	0	3	theme	polysaccharides	89:103	arg1	extraction					23:32	synchronous extraction	11:32	synchronous extraction	11:32	Optimizing synchronous extraction and antioxidant activity evaluation of polyphenols and polysaccharides from Ya'an Tibetan tea (Camellia sinensis).
31993173	0	3	theme	polysaccharides	89:103	arg1	activity					50:57	antioxidant activity	38:57	antioxidant activity	38:57	Optimizing synchronous extraction and antioxidant activity evaluation of polyphenols and polysaccharides from Ya'an Tibetan tea (Camellia sinensis).
31993173	1	4	theme	antioxidative	297:309	arg1	capacity					311:318	the antioxidative capacity	293:318	the antioxidative capacity of TPS and TPP	293:333	The optimal synchronous conditions to extract tea polysaccharides (TPS) and tea polyphenols (TPP) from Ya'an Tibetan tea were investigated, and the antioxidative capacity of TPS and TPP was measured, and the tea was analyzed to identify the polyphenol compounds it contained.
31993173	1	5	theme	optimal	153:159	arg1	conditions					173:182	The optimal synchronous conditions	149:182	The optimal synchronous conditions to extract tea polysaccharides (TPS) and tea polyphenols (TPP) from Ya'an Tibetan tea	149:268	The optimal synchronous conditions to extract tea polysaccharides (TPS) and tea polyphenols (TPP) from Ya'an Tibetan tea were investigated, and the antioxidative capacity of TPS and TPP was measured, and the tea was analyzed to identify the polyphenol compounds it contained.
31993173	1	6	dep	compounds	401:409	arg1	it					411:412	it	411:412	it	411:412	The optimal synchronous conditions to extract tea polysaccharides (TPS) and tea polyphenols (TPP) from Ya'an Tibetan tea were investigated, and the antioxidative capacity of TPS and TPP was measured, and the tea was analyzed to identify the polyphenol compounds it contained.
31993173	3	7	theme	104 min	676:682	arg1	ratio					708:712	liquid-to-material ratio	689:712	liquid-to-material ratio of 41 ml/g	689:723	The optimal extraction technology was determined as extraction temperature of 83°C, time of 104 min, and liquid-to-material ratio of 41 ml/g, yielding TPP and TPS at 42.70 ± 2.38 mg/g and 53.86 ± 3.79 mg/g, respectively.
31993173	3	7	theme	104 min	676:682	arg1	temperature					647:657	extraction temperature	636:657	extraction temperature of 83°C	636:665	The optimal extraction technology was determined as extraction temperature of 83°C, time of 104 min, and liquid-to-material ratio of 41 ml/g, yielding TPP and TPS at 42.70 ± 2.38 mg/g and 53.86 ± 3.79 mg/g, respectively.
31993173	3	7	theme	104 min	676:682	arg1	technology					607:616	The optimal extraction technology	584:616	The optimal extraction technology	584:616	The optimal extraction technology was determined as extraction temperature of 83°C, time of 104 min, and liquid-to-material ratio of 41 ml/g, yielding TPP and TPS at 42.70 ± 2.38 mg/g and 53.86 ± 3.79 mg/g, respectively.
31993173	3	7	theme	104 min	676:682	arg1	time					668:671	time	668:671	time of 104 min	668:682	The optimal extraction technology was determined as extraction temperature of 83°C, time of 104 min, and liquid-to-material ratio of 41 ml/g, yielding TPP and TPS at 42.70 ± 2.38 mg/g and 53.86 ± 3.79 mg/g, respectively.
31993173	0	8	theme	Tibetan	116:122	arg1	Camellia					129:136	Camellia	129:136	Camellia	129:136	Optimizing synchronous extraction and antioxidant activity evaluation of polyphenols and polysaccharides from Ya'an Tibetan tea (Camellia sinensis).
31993173	0	8	theme	Tibetan	116:122	arg1	tea					124:126	Ya'an Tibetan tea	110:126	Ya'an Tibetan tea (Camellia sinensis)	110:146	Optimizing synchronous extraction and antioxidant activity evaluation of polyphenols and polysaccharides from Ya'an Tibetan tea (Camellia sinensis).
31993173	1	9	theme	TPS	323:325	arg1	capacity					311:318	the antioxidative capacity	293:318	the antioxidative capacity of TPS and TPP	293:333	The optimal synchronous conditions to extract tea polysaccharides (TPS) and tea polyphenols (TPP) from Ya'an Tibetan tea were investigated, and the antioxidative capacity of TPS and TPP was measured, and the tea was analyzed to identify the polyphenol compounds it contained.
31993173	1	10	theme	polyphenol	390:399	arg1	compounds					401:409	the polyphenol compounds	386:409	the polyphenol compounds it contained	386:422	The optimal synchronous conditions to extract tea polysaccharides (TPS) and tea polyphenols (TPP) from Ya'an Tibetan tea were investigated, and the antioxidative capacity of TPS and TPP was measured, and the tea was analyzed to identify the polyphenol compounds it contained.
31993173	4	11	contain	have	842:845	arg1	TPS					809:811	TPS	809:811	TPS	809:811	The TPS and TPP in Ya'an Tibetan tea have high eliminating activities on DPPH and strong reducing power, with TPP showing a higher antioxidant activity than TPS.
31993173	4	11	contain	have	842:845	arg2	activities					864:873	high eliminating activities	847:873	high eliminating activities	847:873	The TPS and TPP in Ya'an Tibetan tea have high eliminating activities on DPPH and strong reducing power, with TPP showing a higher antioxidant activity than TPS.
31993173	4	11	contain	have	842:845	arg1	TPP					817:819	TPP	817:819	TPP	817:819	The TPS and TPP in Ya'an Tibetan tea have high eliminating activities on DPPH and strong reducing power, with TPP showing a higher antioxidant activity than TPS.
31993173	1	12	theme	synchronous	161:171	arg1	conditions					173:182	The optimal synchronous conditions	149:182	The optimal synchronous conditions to extract tea polysaccharides (TPS) and tea polyphenols (TPP) from Ya'an Tibetan tea	149:268	The optimal synchronous conditions to extract tea polysaccharides (TPS) and tea polyphenols (TPP) from Ya'an Tibetan tea were investigated, and the antioxidative capacity of TPS and TPP was measured, and the tea was analyzed to identify the polyphenol compounds it contained.
31993173	2	13	theme	single-factor	441:453	arg1	experiments					455:465	single-factor experiments	441:465	single-factor experiments	441:465	On the basis of single-factor experiments, a Box-Behnken design and response surface methodology were applied to optimize the hot water extraction conditions.
31993173	5	14	theme	major	1025:1029	arg1	GCG					1009:1011	GCG	1009:1011	GCG	1009:1011	UHPLC-QqQ-MS/MS analysis identified EGCG, GCG, and ECG as major polyphenol components in Ya'an Tibetan tea.
31993173	5	14	theme	major	1025:1029	arg1	ECG					1018:1020	ECG	1018:1020	ECG	1018:1020	UHPLC-QqQ-MS/MS analysis identified EGCG, GCG, and ECG as major polyphenol components in Ya'an Tibetan tea.
31993173	5	14	theme	major	1025:1029	arg1	EGCG					1003:1006	EGCG	1003:1006	EGCG	1003:1006	UHPLC-QqQ-MS/MS analysis identified EGCG, GCG, and ECG as major polyphenol components in Ya'an Tibetan tea.
31993173	5	14	theme	major	1025:1029	arg1	components					1042:1051	major polyphenol components	1025:1051	major polyphenol components in Ya'an Tibetan tea	1025:1072	UHPLC-QqQ-MS/MS analysis identified EGCG, GCG, and ECG as major polyphenol components in Ya'an Tibetan tea.
31993173	6	15	theme	food	1148:1151	arg1	industry					1153:1160	the food industry	1144:1160	the food industry	1144:1160	These findings might promote the application of Ya'an Tibetan tea in the food industry.
31993173	4	16	theme	reducing	894:901	arg1	power					903:907	strong reducing power	887:907	strong reducing power	887:907	The TPS and TPP in Ya'an Tibetan tea have high eliminating activities on DPPH and strong reducing power, with TPP showing a higher antioxidant activity than TPS.
31993173	0	17	theme	synchronous	11:21	arg1	extraction					23:32	synchronous extraction	11:32	synchronous extraction	11:32	Optimizing synchronous extraction and antioxidant activity evaluation of polyphenols and polysaccharides from Ya'an Tibetan tea (Camellia sinensis).
31993173	1	18	theme	TPP	331:333	arg1	capacity					311:318	the antioxidative capacity	293:318	the antioxidative capacity of TPS and TPP	293:333	The optimal synchronous conditions to extract tea polysaccharides (TPS) and tea polyphenols (TPP) from Ya'an Tibetan tea were investigated, and the antioxidative capacity of TPS and TPP was measured, and the tea was analyzed to identify the polyphenol compounds it contained.
31993173	2	19	theme	extraction	561:570	arg1	conditions					572:581	the hot water extraction conditions	547:581	the hot water extraction conditions	547:581	On the basis of single-factor experiments, a Box-Behnken design and response surface methodology were applied to optimize the hot water extraction conditions.
31993173	4	20	dep	TPS	809:811	arg1	The					805:807	The	805:807	The	805:807	The TPS and TPP in Ya'an Tibetan tea have high eliminating activities on DPPH and strong reducing power, with TPP showing a higher antioxidant activity than TPS.
31993173	2	21	theme	water	555:559	arg1	conditions					572:581	the hot water extraction conditions	547:581	the hot water extraction conditions	547:581	On the basis of single-factor experiments, a Box-Behnken design and response surface methodology were applied to optimize the hot water extraction conditions.
31993173	1	22	theme	extract	187:193	arg1	TPP					242:244	TPP	242:244	TPP	242:244	The optimal synchronous conditions to extract tea polysaccharides (TPS) and tea polyphenols (TPP) from Ya'an Tibetan tea were investigated, and the antioxidative capacity of TPS and TPP was measured, and the tea was analyzed to identify the polyphenol compounds it contained.
31993173	1	22	theme	extract	187:193	arg1	TPS					216:218	extract tea polysaccharides (TPS) and tea polyphenols	187:239	TPS	216:218	The optimal synchronous conditions to extract tea polysaccharides (TPS) and tea polyphenols (TPP) from Ya'an Tibetan tea were investigated, and the antioxidative capacity of TPS and TPP was measured, and the tea was analyzed to identify the polyphenol compounds it contained.
31993173	2	23	theme	response	493:500	arg1	methodology					510:520	response surface methodology	493:520	response surface methodology	493:520	On the basis of single-factor experiments, a Box-Behnken design and response surface methodology were applied to optimize the hot water extraction conditions.
31993173	1	24	theme	tea	195:197	arg1	TPP					242:244	TPP	242:244	TPP	242:244	The optimal synchronous conditions to extract tea polysaccharides (TPS) and tea polyphenols (TPP) from Ya'an Tibetan tea were investigated, and the antioxidative capacity of TPS and TPP was measured, and the tea was analyzed to identify the polyphenol compounds it contained.
31993173	1	24	theme	tea	195:197	arg1	TPS					216:218	extract tea polysaccharides (TPS) and tea polyphenols	187:239	TPS	216:218	The optimal synchronous conditions to extract tea polysaccharides (TPS) and tea polyphenols (TPP) from Ya'an Tibetan tea were investigated, and the antioxidative capacity of TPS and TPP was measured, and the tea was analyzed to identify the polyphenol compounds it contained.
31993173	4	25	theme	high	847:850	arg1	activities					864:873	high eliminating activities	847:873	high eliminating activities	847:873	The TPS and TPP in Ya'an Tibetan tea have high eliminating activities on DPPH and strong reducing power, with TPP showing a higher antioxidant activity than TPS.
31993173	3	26	theme	extraction	596:605	arg1	ratio					708:712	liquid-to-material ratio	689:712	liquid-to-material ratio of 41 ml/g	689:723	The optimal extraction technology was determined as extraction temperature of 83°C, time of 104 min, and liquid-to-material ratio of 41 ml/g, yielding TPP and TPS at 42.70 ± 2.38 mg/g and 53.86 ± 3.79 mg/g, respectively.
31993173	3	26	theme	extraction	596:605	arg1	temperature					647:657	extraction temperature	636:657	extraction temperature of 83°C	636:665	The optimal extraction technology was determined as extraction temperature of 83°C, time of 104 min, and liquid-to-material ratio of 41 ml/g, yielding TPP and TPS at 42.70 ± 2.38 mg/g and 53.86 ± 3.79 mg/g, respectively.
31993173	3	26	theme	extraction	596:605	arg1	technology					607:616	The optimal extraction technology	584:616	The optimal extraction technology	584:616	The optimal extraction technology was determined as extraction temperature of 83°C, time of 104 min, and liquid-to-material ratio of 41 ml/g, yielding TPP and TPS at 42.70 ± 2.38 mg/g and 53.86 ± 3.79 mg/g, respectively.
31993173	3	26	theme	extraction	596:605	arg1	time					668:671	time	668:671	time of 104 min	668:682	The optimal extraction technology was determined as extraction temperature of 83°C, time of 104 min, and liquid-to-material ratio of 41 ml/g, yielding TPP and TPS at 42.70 ± 2.38 mg/g and 53.86 ± 3.79 mg/g, respectively.
31993173	1	27	theme	polysaccharides	199:213	arg1	TPP					242:244	TPP	242:244	TPP	242:244	The optimal synchronous conditions to extract tea polysaccharides (TPS) and tea polyphenols (TPP) from Ya'an Tibetan tea were investigated, and the antioxidative capacity of TPS and TPP was measured, and the tea was analyzed to identify the polyphenol compounds it contained.
31993173	1	27	theme	polysaccharides	199:213	arg1	TPS					216:218	extract tea polysaccharides (TPS) and tea polyphenols	187:239	TPS	216:218	The optimal synchronous conditions to extract tea polysaccharides (TPS) and tea polyphenols (TPP) from Ya'an Tibetan tea were investigated, and the antioxidative capacity of TPS and TPP was measured, and the tea was analyzed to identify the polyphenol compounds it contained.
31993173	1	28	theme	Tibetan	258:264	arg1	tea					266:268	Ya'an Tibetan tea	252:268	Ya'an Tibetan tea	252:268	The optimal synchronous conditions to extract tea polysaccharides (TPS) and tea polyphenols (TPP) from Ya'an Tibetan tea were investigated, and the antioxidative capacity of TPS and TPP was measured, and the tea was analyzed to identify the polyphenol compounds it contained.
31993173	5	29	from	components	1042:1051	arg1	tea					1070:1072	Ya'an Tibetan tea	1056:1072	Ya'an Tibetan tea	1056:1072	UHPLC-QqQ-MS/MS analysis identified EGCG, GCG, and ECG as major polyphenol components in Ya'an Tibetan tea.
31993173	0	30	theme	antioxidant	38:48	arg1	activity					50:57	antioxidant activity	38:57	antioxidant activity	38:57	Optimizing synchronous extraction and antioxidant activity evaluation of polyphenols and polysaccharides from Ya'an Tibetan tea (Camellia sinensis).
31993173	2	31	theme	Box-Behnken	470:480	arg1	design					482:487	a Box-Behnken design	468:487	a Box-Behnken design	468:487	On the basis of single-factor experiments, a Box-Behnken design and response surface methodology were applied to optimize the hot water extraction conditions.
31993173	6	32	theme	Tibetan	1129:1135	arg1	tea					1137:1139	Ya'an Tibetan tea	1123:1139	Ya'an Tibetan tea	1123:1139	These findings might promote the application of Ya'an Tibetan tea in the food industry.
31993173	4	33	theme	strong	887:892	arg1	power					903:907	strong reducing power	887:907	strong reducing power	887:907	The TPS and TPP in Ya'an Tibetan tea have high eliminating activities on DPPH and strong reducing power, with TPP showing a higher antioxidant activity than TPS.
31993173	4	34	theme	antioxidant	936:946	arg1	activity					948:955	a higher antioxidant activity	927:955	a higher antioxidant activity than TPS	927:964	The TPS and TPP in Ya'an Tibetan tea have high eliminating activities on DPPH and strong reducing power, with TPP showing a higher antioxidant activity than TPS.
31993173	5	35	theme	Tibetan	1062:1068	arg1	tea					1070:1072	Ya'an Tibetan tea	1056:1072	Ya'an Tibetan tea	1056:1072	UHPLC-QqQ-MS/MS analysis identified EGCG, GCG, and ECG as major polyphenol components in Ya'an Tibetan tea.
31993173	4	36	theme	Tibetan	830:836	arg1	tea					838:840	Ya'an Tibetan tea	824:840	Ya'an Tibetan tea	824:840	The TPS and TPP in Ya'an Tibetan tea have high eliminating activities on DPPH and strong reducing power, with TPP showing a higher antioxidant activity than TPS.
31993173	3	37	theme	optimal	588:594	arg1	ratio					708:712	liquid-to-material ratio	689:712	liquid-to-material ratio of 41 ml/g	689:723	The optimal extraction technology was determined as extraction temperature of 83°C, time of 104 min, and liquid-to-material ratio of 41 ml/g, yielding TPP and TPS at 42.70 ± 2.38 mg/g and 53.86 ± 3.79 mg/g, respectively.
31993173	3	37	theme	optimal	588:594	arg1	temperature					647:657	extraction temperature	636:657	extraction temperature of 83°C	636:665	The optimal extraction technology was determined as extraction temperature of 83°C, time of 104 min, and liquid-to-material ratio of 41 ml/g, yielding TPP and TPS at 42.70 ± 2.38 mg/g and 53.86 ± 3.79 mg/g, respectively.
31993173	3	37	theme	optimal	588:594	arg1	technology					607:616	The optimal extraction technology	584:616	The optimal extraction technology	584:616	The optimal extraction technology was determined as extraction temperature of 83°C, time of 104 min, and liquid-to-material ratio of 41 ml/g, yielding TPP and TPS at 42.70 ± 2.38 mg/g and 53.86 ± 3.79 mg/g, respectively.
31993173	3	37	theme	optimal	588:594	arg1	time					668:671	time	668:671	time of 104 min	668:682	The optimal extraction technology was determined as extraction temperature of 83°C, time of 104 min, and liquid-to-material ratio of 41 ml/g, yielding TPP and TPS at 42.70 ± 2.38 mg/g and 53.86 ± 3.79 mg/g, respectively.
31993173	4	38	theme	higher	929:934	arg1	activity					948:955	a higher antioxidant activity	927:955	a higher antioxidant activity than TPS	927:964	The TPS and TPP in Ya'an Tibetan tea have high eliminating activities on DPPH and strong reducing power, with TPP showing a higher antioxidant activity than TPS.
31993173	3	39	theme	41 ml/g	717:723	arg1	ratio					708:712	liquid-to-material ratio	689:712	liquid-to-material ratio of 41 ml/g	689:723	The optimal extraction technology was determined as extraction temperature of 83°C, time of 104 min, and liquid-to-material ratio of 41 ml/g, yielding TPP and TPS at 42.70 ± 2.38 mg/g and 53.86 ± 3.79 mg/g, respectively.
31993173	3	39	theme	41 ml/g	717:723	arg1	temperature					647:657	extraction temperature	636:657	extraction temperature of 83°C	636:665	The optimal extraction technology was determined as extraction temperature of 83°C, time of 104 min, and liquid-to-material ratio of 41 ml/g, yielding TPP and TPS at 42.70 ± 2.38 mg/g and 53.86 ± 3.79 mg/g, respectively.
31993173	3	39	theme	41 ml/g	717:723	arg1	technology					607:616	The optimal extraction technology	584:616	The optimal extraction technology	584:616	The optimal extraction technology was determined as extraction temperature of 83°C, time of 104 min, and liquid-to-material ratio of 41 ml/g, yielding TPP and TPS at 42.70 ± 2.38 mg/g and 53.86 ± 3.79 mg/g, respectively.
31993173	3	39	theme	41 ml/g	717:723	arg1	time					668:671	time	668:671	time of 104 min	668:682	The optimal extraction technology was determined as extraction temperature of 83°C, time of 104 min, and liquid-to-material ratio of 41 ml/g, yielding TPP and TPS at 42.70 ± 2.38 mg/g and 53.86 ± 3.79 mg/g, respectively.
31993173	2	40	dep	experiments	455:465	arg1	basis					432:436	basis	432:436	basis	432:436	On the basis of single-factor experiments, a Box-Behnken design and response surface methodology were applied to optimize the hot water extraction conditions.
31993173	2	40	dep	experiments	455:465	arg1	the					428:430	the	428:430	the	428:430	On the basis of single-factor experiments, a Box-Behnken design and response surface methodology were applied to optimize the hot water extraction conditions.
31993173	5	41	theme	UHPLC-QqQ-MS/MS	967:981	arg1	analysis					983:990	UHPLC-QqQ-MS/MS analysis	967:990	UHPLC-QqQ-MS/MS analysis	967:990	UHPLC-QqQ-MS/MS analysis identified EGCG, GCG, and ECG as major polyphenol components in Ya'an Tibetan tea.
31993173	3	42	theme	extraction	636:645	arg1	temperature					647:657	extraction temperature	636:657	extraction temperature of 83°C	636:665	The optimal extraction technology was determined as extraction temperature of 83°C, time of 104 min, and liquid-to-material ratio of 41 ml/g, yielding TPP and TPS at 42.70 ± 2.38 mg/g and 53.86 ± 3.79 mg/g, respectively.
31993173	3	42	theme	extraction	636:645	arg1	technology					607:616	The optimal extraction technology	584:616	The optimal extraction technology	584:616	The optimal extraction technology was determined as extraction temperature of 83°C, time of 104 min, and liquid-to-material ratio of 41 ml/g, yielding TPP and TPS at 42.70 ± 2.38 mg/g and 53.86 ± 3.79 mg/g, respectively.
31993173	2	43	theme	surface	502:508	arg1	methodology					510:520	response surface methodology	493:520	response surface methodology	493:520	On the basis of single-factor experiments, a Box-Behnken design and response surface methodology were applied to optimize the hot water extraction conditions.
31993173	0	44	dep	extraction	23:32	arg1	evaluation					59:68	evaluation	59:68	evaluation	59:68	Optimizing synchronous extraction and antioxidant activity evaluation of polyphenols and polysaccharides from Ya'an Tibetan tea (Camellia sinensis).
31993173	4	45	theme	eliminating	852:862	arg1	activities					864:873	high eliminating activities	847:873	high eliminating activities	847:873	The TPS and TPP in Ya'an Tibetan tea have high eliminating activities on DPPH and strong reducing power, with TPP showing a higher antioxidant activity than TPS.
31993173	1	46	theme	tea	225:227	arg1	polyphenols					229:239	extract tea polysaccharides (TPS) and tea polyphenols	187:239	polyphenols	229:239	The optimal synchronous conditions to extract tea polysaccharides (TPS) and tea polyphenols (TPP) from Ya'an Tibetan tea were investigated, and the antioxidative capacity of TPS and TPP was measured, and the tea was analyzed to identify the polyphenol compounds it contained.
31993173	0	47	theme	polyphenols	73:83	arg1	extraction					23:32	synchronous extraction	11:32	synchronous extraction	11:32	Optimizing synchronous extraction and antioxidant activity evaluation of polyphenols and polysaccharides from Ya'an Tibetan tea (Camellia sinensis).
31993173	0	47	theme	polyphenols	73:83	arg1	activity					50:57	antioxidant activity	38:57	antioxidant activity	38:57	Optimizing synchronous extraction and antioxidant activity evaluation of polyphenols and polysaccharides from Ya'an Tibetan tea (Camellia sinensis).
31993173	3	48	theme	83°C	662:665	arg1	ratio					708:712	liquid-to-material ratio	689:712	liquid-to-material ratio of 41 ml/g	689:723	The optimal extraction technology was determined as extraction temperature of 83°C, time of 104 min, and liquid-to-material ratio of 41 ml/g, yielding TPP and TPS at 42.70 ± 2.38 mg/g and 53.86 ± 3.79 mg/g, respectively.
31993173	3	48	theme	83°C	662:665	arg1	temperature					647:657	extraction temperature	636:657	extraction temperature of 83°C	636:665	The optimal extraction technology was determined as extraction temperature of 83°C, time of 104 min, and liquid-to-material ratio of 41 ml/g, yielding TPP and TPS at 42.70 ± 2.38 mg/g and 53.86 ± 3.79 mg/g, respectively.
31993173	3	48	theme	83°C	662:665	arg1	technology					607:616	The optimal extraction technology	584:616	The optimal extraction technology	584:616	The optimal extraction technology was determined as extraction temperature of 83°C, time of 104 min, and liquid-to-material ratio of 41 ml/g, yielding TPP and TPS at 42.70 ± 2.38 mg/g and 53.86 ± 3.79 mg/g, respectively.
31993173	3	48	theme	83°C	662:665	arg1	time					668:671	time	668:671	time of 104 min	668:682	The optimal extraction technology was determined as extraction temperature of 83°C, time of 104 min, and liquid-to-material ratio of 41 ml/g, yielding TPP and TPS at 42.70 ± 2.38 mg/g and 53.86 ± 3.79 mg/g, respectively.
31993173	0	49	dep	Camellia	129:136	arg1	sinensis					138:145	Camellia sinensis	129:145	Camellia sinensis	129:145	Optimizing synchronous extraction and antioxidant activity evaluation of polyphenols and polysaccharides from Ya'an Tibetan tea (Camellia sinensis).
30063198	2	0	theme	milky-white	105:115	arg1	bacterium					151:159	A Gram-positive, strictly aerobic, non-motile, milky-white to creamy coloured and rod-shaped bacterium	58:159	A Gram-positive, strictly aerobic, non-motile, milky-white to creamy coloured and rod-shaped bacterium	58:159	A Gram-positive, strictly aerobic, non-motile, milky-white to creamy coloured and rod-shaped bacterium, designated BS05T, was isolated from compost.
30063198	3	1	theme	Phylogenetic	207:218	arg1	analysis					220:227	Phylogenetic analysis	207:227	Phylogenetic analysis based on 16S rRNA gene sequence comparison	207:270	Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that the strain formed a distinct lineage within the genus Brevibacterium and was most closely related to Brevibacterium avium NCFB 3055T (96.3 %), Brevibacterium oceani BBH7T (96.2 %) and Brevibacterium epidermidis NBRC 14811T (96.1 %).
30063198	9	2	theme	genus	1040:1044	arg1	Brevibacterium					1046:1059	the genus Brevibacterium	1036:1059	the genus Brevibacterium	1036:1059	Moreover, the low sequence similarity of the 16S rRNA gene sequencing, physiological, biochemical and chemotaxonomic analyses allowed the phenotypic and genotypic differentiation of strain BS05T from the recognized species of the genus Brevibacterium.
30063198	3	3	theme	distinct	306:313	arg1	lineage					315:321	a distinct lineage	304:321	a distinct lineage	304:321	Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that the strain formed a distinct lineage within the genus Brevibacterium and was most closely related to Brevibacterium avium NCFB 3055T (96.3 %), Brevibacterium oceani BBH7T (96.2 %) and Brevibacterium epidermidis NBRC 14811T (96.1 %).
30063198	8	4	theme	major	766:770	arg1	phosphatidylglycerol					788:807	phosphatidylglycerol	788:807	phosphatidylglycerol	788:807	The major polar lipid was phosphatidylglycerol.
30063198	8	4	theme	major	766:770	arg1	lipid					778:782	The major polar lipid	762:782	The major polar lipid	762:782	The major polar lipid was phosphatidylglycerol.
30063198	4	5	theme	DNA	523:525	arg1	G+C content					527:537	The DNA G+C content	519:537	The DNA G+C content	519:537	The DNA G+C content was 62.3 mol%.
30063198	4	5	theme	DNA	523:525	arg1	%					551:551	62.3 mol%	543:551	62.3 mol%	543:551	The DNA G+C content was 62.3 mol%.
30063198	2	6	theme	non-motile	93:102	arg1	bacterium					151:159	A Gram-positive, strictly aerobic, non-motile, milky-white to creamy coloured and rod-shaped bacterium	58:159	A Gram-positive, strictly aerobic, non-motile, milky-white to creamy coloured and rod-shaped bacterium	58:159	A Gram-positive, strictly aerobic, non-motile, milky-white to creamy coloured and rod-shaped bacterium, designated BS05T, was isolated from compost.
30063198	9	7	theme	biochemical	896:906	arg1	analyses					927:934	physiological, biochemical and chemotaxonomic analyses	881:934	physiological, biochemical and chemotaxonomic analyses	881:934	Moreover, the low sequence similarity of the 16S rRNA gene sequencing, physiological, biochemical and chemotaxonomic analyses allowed the phenotypic and genotypic differentiation of strain BS05T from the recognized species of the genus Brevibacterium.
30063198	9	8	theme	strain	992:997	arg1	BS05T					999:1003	strain BS05T	992:1003	strain BS05T	992:1003	Moreover, the low sequence similarity of the 16S rRNA gene sequencing, physiological, biochemical and chemotaxonomic analyses allowed the phenotypic and genotypic differentiation of strain BS05T from the recognized species of the genus Brevibacterium.
30063198	4	9	theme	62.3 mol	543:550	arg1	G+C content					527:537	The DNA G+C content	519:537	The DNA G+C content	519:537	The DNA G+C content was 62.3 mol%.
30063198	4	9	theme	62.3 mol	543:550	arg1	%					551:551	62.3 mol%	543:551	62.3 mol%	543:551	The DNA G+C content was 62.3 mol%.
30063198	9	10	theme	16S	855:857	arg1	sequencing					869:878	the 16S rRNA gene sequencing	851:878	the 16S rRNA gene sequencing	851:878	Moreover, the low sequence similarity of the 16S rRNA gene sequencing, physiological, biochemical and chemotaxonomic analyses allowed the phenotypic and genotypic differentiation of strain BS05T from the recognized species of the genus Brevibacterium.
30063198	2	11	theme	rod-shaped	140:149	arg1	bacterium					151:159	A Gram-positive, strictly aerobic, non-motile, milky-white to creamy coloured and rod-shaped bacterium	58:159	A Gram-positive, strictly aerobic, non-motile, milky-white to creamy coloured and rod-shaped bacterium	58:159	A Gram-positive, strictly aerobic, non-motile, milky-white to creamy coloured and rod-shaped bacterium, designated BS05T, was isolated from compost.
30063198	7	12	theme	strain	713:718	arg1	BS05T					720:724	strain BS05T	713:724	strain BS05T	713:724	The cell-wall peptidoglycan of strain BS05T contained meso-diaminopimelic acid.
30063198	9	13	theme	BS05T	999:1003	arg1	differentiation					973:987	the phenotypic and genotypic differentiation	944:987	the phenotypic and genotypic differentiation of strain BS05T from the recognized species of the genus Brevibacterium	944:1059	Moreover, the low sequence similarity of the 16S rRNA gene sequencing, physiological, biochemical and chemotaxonomic analyses allowed the phenotypic and genotypic differentiation of strain BS05T from the recognized species of the genus Brevibacterium.
30063198	10	14	theme	genus	1120:1124	arg1	Brevibacterium					1126:1139	the genus Brevibacterium	1116:1139	the genus Brevibacterium	1116:1139	Therefore, strain BS05T represents a novel species of the genus Brevibacterium, for which the name Brevibacteriumhankyongi sp.
30063198	9	15	theme	rRNA	859:862	arg1	sequencing					869:878	the 16S rRNA gene sequencing	851:878	the 16S rRNA gene sequencing	851:878	Moreover, the low sequence similarity of the 16S rRNA gene sequencing, physiological, biochemical and chemotaxonomic analyses allowed the phenotypic and genotypic differentiation of strain BS05T from the recognized species of the genus Brevibacterium.
30063198	3	16	theme	16S	238:240	arg1	rRNA					242:245	16S rRNA	238:245	16S rRNA gene sequence comparison	238:270	Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that the strain formed a distinct lineage within the genus Brevibacterium and was most closely related to Brevibacterium avium NCFB 3055T (96.3 %), Brevibacterium oceani BBH7T (96.2 %) and Brevibacterium epidermidis NBRC 14811T (96.1 %).
30063198	5	17	theme	predominant	558:568	arg1	MK-8					582:585	MK-8	582:585	MK-8	582:585	The predominant quinone was MK-8(H2).
30063198	5	17	theme	predominant	558:568	arg1	quinone					570:576	The predominant quinone	554:576	The predominant quinone	554:576	The predominant quinone was MK-8(H2).
30063198	3	18	theme	NBRC	497:500	arg1	14811T					502:507	Brevibacterium epidermidis NBRC 14811T	470:507	Brevibacterium epidermidis NBRC 14811T (96.1 %)	470:516	Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that the strain formed a distinct lineage within the genus Brevibacterium and was most closely related to Brevibacterium avium NCFB 3055T (96.3 %), Brevibacterium oceani BBH7T (96.2 %) and Brevibacterium epidermidis NBRC 14811T (96.1 %).
30063198	3	18	theme	NBRC	497:500	arg1	%					515:515	96.1 %	510:515	96.1 %	510:515	Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that the strain formed a distinct lineage within the genus Brevibacterium and was most closely related to Brevibacterium avium NCFB 3055T (96.3 %), Brevibacterium oceani BBH7T (96.2 %) and Brevibacterium epidermidis NBRC 14811T (96.1 %).
30063198	8	19	theme	polar	772:776	arg1	phosphatidylglycerol					788:807	phosphatidylglycerol	788:807	phosphatidylglycerol	788:807	The major polar lipid was phosphatidylglycerol.
30063198	8	19	theme	polar	772:776	arg1	lipid					778:782	The major polar lipid	762:782	The major polar lipid	762:782	The major polar lipid was phosphatidylglycerol.
30063198	2	20	attach	isolated	184:191	arg2	bacterium					151:159	A Gram-positive, strictly aerobic, non-motile, milky-white to creamy coloured and rod-shaped bacterium	58:159	A Gram-positive, strictly aerobic, non-motile, milky-white to creamy coloured and rod-shaped bacterium	58:159	A Gram-positive, strictly aerobic, non-motile, milky-white to creamy coloured and rod-shaped bacterium, designated BS05T, was isolated from compost.
30063198	2	20	attach	isolated	184:191	arg1	compost					198:204	compost	198:204	compost	198:204	A Gram-positive, strictly aerobic, non-motile, milky-white to creamy coloured and rod-shaped bacterium, designated BS05T, was isolated from compost.
30063198	3	21	theme	genus	334:338	arg1	Brevibacterium					340:353	the genus Brevibacterium	330:353	the genus Brevibacterium	330:353	Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that the strain formed a distinct lineage within the genus Brevibacterium and was most closely related to Brevibacterium avium NCFB 3055T (96.3 %), Brevibacterium oceani BBH7T (96.2 %) and Brevibacterium epidermidis NBRC 14811T (96.1 %).
30063198	2	22	dep	Gram-positive	60:72	arg1	coloured					127:134	coloured	127:134	coloured	127:134	A Gram-positive, strictly aerobic, non-motile, milky-white to creamy coloured and rod-shaped bacterium, designated BS05T, was isolated from compost.
30063198	2	22	dep	Gram-positive	60:72	arg1	to					117:118	to	117:118	to	117:118	A Gram-positive, strictly aerobic, non-motile, milky-white to creamy coloured and rod-shaped bacterium, designated BS05T, was isolated from compost.
30063198	3	23	theme	rRNA	242:245	arg1	comparison					261:270	16S rRNA gene sequence comparison	238:270	16S rRNA gene sequence comparison	238:270	Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that the strain formed a distinct lineage within the genus Brevibacterium and was most closely related to Brevibacterium avium NCFB 3055T (96.3 %), Brevibacterium oceani BBH7T (96.2 %) and Brevibacterium epidermidis NBRC 14811T (96.1 %).
30063198	10	24	theme	Brevibacterium	1126:1139	arg1	species					1105:1111	a novel species	1097:1111	a novel species	1097:1111	Therefore, strain BS05T represents a novel species of the genus Brevibacterium, for which the name Brevibacteriumhankyongi sp.
30063198	0	25	theme	hankyongi	15:23	arg1	sp					25:26	Brevibacterium hankyongi sp	0:26	Brevibacterium hankyongi sp.	0:27	Brevibacterium hankyongi sp.
30063198	11	26	theme	type	1216:1219	arg1	29562T					1252:1257	=KACC 18875T=LMG 29562T	1235:1257	=KACC 18875T=LMG 29562T	1235:1257	nov. is proposed, with the type strain BS05T (=KACC 18875T=LMG 29562T).
30063198	11	26	theme	type	1216:1219	arg1	BS05T					1228:1232	the type strain BS05T	1212:1232	the type strain BS05T (=KACC 18875T=LMG 29562T)	1212:1258	nov. is proposed, with the type strain BS05T (=KACC 18875T=LMG 29562T).
30063198	6	27	dep	anteiso-C15 	619:630	arg1	 0					678:679	 0	678:679	 0	678:679	The major fatty acids were anteiso-C15 : 0, anteiso-C17 : 0, iso-C16 : 0 and iso-C15 : 0.
30063198	6	27	dep	anteiso-C15 	619:630	arg1	 0					632:633	 0	632:633	 0	632:633	The major fatty acids were anteiso-C15 : 0, anteiso-C17 : 0, iso-C16 : 0 and iso-C15 : 0.
30063198	6	27	dep	anteiso-C15 	619:630	arg1	iso-C15 					669:676	iso-C15 	669:676	iso-C15 	669:676	The major fatty acids were anteiso-C15 : 0, anteiso-C17 : 0, iso-C16 : 0 and iso-C15 : 0.
30063198	6	27	dep	anteiso-C15 	619:630	arg1	 0					662:663	 0	662:663	 0	662:663	The major fatty acids were anteiso-C15 : 0, anteiso-C17 : 0, iso-C16 : 0 and iso-C15 : 0.
30063198	6	27	dep	anteiso-C15 	619:630	arg1	 0					649:650	 0	649:650	 0	649:650	The major fatty acids were anteiso-C15 : 0, anteiso-C17 : 0, iso-C16 : 0 and iso-C15 : 0.
30063198	9	28	theme	recognized	1014:1023	arg1	species					1025:1031	the recognized species	1010:1031	the recognized species of the genus Brevibacterium	1010:1059	Moreover, the low sequence similarity of the 16S rRNA gene sequencing, physiological, biochemical and chemotaxonomic analyses allowed the phenotypic and genotypic differentiation of strain BS05T from the recognized species of the genus Brevibacterium.
30063198	0	29	theme	Brevibacterium	0:13	arg1	sp					25:26	Brevibacterium hankyongi sp	0:26	Brevibacterium hankyongi sp.	0:27	Brevibacterium hankyongi sp.
30063198	2	30	theme	Gram-positive	60:72	arg1	bacterium					151:159	A Gram-positive, strictly aerobic, non-motile, milky-white to creamy coloured and rod-shaped bacterium	58:159	A Gram-positive, strictly aerobic, non-motile, milky-white to creamy coloured and rod-shaped bacterium	58:159	A Gram-positive, strictly aerobic, non-motile, milky-white to creamy coloured and rod-shaped bacterium, designated BS05T, was isolated from compost.
30063198	11	31	theme	strain	1221:1226	arg1	29562T					1252:1257	=KACC 18875T=LMG 29562T	1235:1257	=KACC 18875T=LMG 29562T	1235:1257	nov. is proposed, with the type strain BS05T (=KACC 18875T=LMG 29562T).
30063198	11	31	theme	strain	1221:1226	arg1	BS05T					1228:1232	the type strain BS05T	1212:1232	the type strain BS05T (=KACC 18875T=LMG 29562T)	1212:1258	nov. is proposed, with the type strain BS05T (=KACC 18875T=LMG 29562T).
30063198	7	32	contain	contained	726:734	arg1	peptidoglycan					696:708	The cell-wall peptidoglycan	682:708	The cell-wall peptidoglycan of strain BS05T	682:724	The cell-wall peptidoglycan of strain BS05T contained meso-diaminopimelic acid.
30063198	7	32	contain	contained	726:734	arg2	acid					756:759	meso-diaminopimelic acid	736:759	meso-diaminopimelic acid	736:759	The cell-wall peptidoglycan of strain BS05T contained meso-diaminopimelic acid.
30063198	9	33	theme	physiological	881:893	arg1	analyses					927:934	physiological, biochemical and chemotaxonomic analyses	881:934	physiological, biochemical and chemotaxonomic analyses	881:934	Moreover, the low sequence similarity of the 16S rRNA gene sequencing, physiological, biochemical and chemotaxonomic analyses allowed the phenotypic and genotypic differentiation of strain BS05T from the recognized species of the genus Brevibacterium.
30063198	7	34	theme	meso-diaminopimelic	736:754	arg1	acid					756:759	meso-diaminopimelic acid	736:759	meso-diaminopimelic acid	736:759	The cell-wall peptidoglycan of strain BS05T contained meso-diaminopimelic acid.
30063198	3	35	theme	Brevibacterium	429:442	arg1	BBH7T					451:455	Brevibacterium oceani BBH7T	429:455	Brevibacterium oceani BBH7T (96.2 %)	429:464	Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that the strain formed a distinct lineage within the genus Brevibacterium and was most closely related to Brevibacterium avium NCFB 3055T (96.3 %), Brevibacterium oceani BBH7T (96.2 %) and Brevibacterium epidermidis NBRC 14811T (96.1 %).
30063198	3	35	theme	Brevibacterium	429:442	arg1	%					463:463	96.2 %	458:463	96.2 %	458:463	Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that the strain formed a distinct lineage within the genus Brevibacterium and was most closely related to Brevibacterium avium NCFB 3055T (96.3 %), Brevibacterium oceani BBH7T (96.2 %) and Brevibacterium epidermidis NBRC 14811T (96.1 %).
30063198	11	36	theme	18875T=LMG	1241:1250	arg1	29562T					1252:1257	=KACC 18875T=LMG 29562T	1235:1257	=KACC 18875T=LMG 29562T	1235:1257	nov. is proposed, with the type strain BS05T (=KACC 18875T=LMG 29562T).
30063198	11	36	theme	18875T=LMG	1241:1250	arg1	BS05T					1228:1232	the type strain BS05T	1212:1232	the type strain BS05T (=KACC 18875T=LMG 29562T)	1212:1258	nov. is proposed, with the type strain BS05T (=KACC 18875T=LMG 29562T).
30063198	7	37	theme	BS05T	720:724	arg1	peptidoglycan					696:708	The cell-wall peptidoglycan	682:708	The cell-wall peptidoglycan of strain BS05T	682:724	The cell-wall peptidoglycan of strain BS05T contained meso-diaminopimelic acid.
30063198	6	38	theme	fatty	602:606	arg1	acids					608:612	The major fatty acids	592:612	The major fatty acids	592:612	The major fatty acids were anteiso-C15 : 0, anteiso-C17 : 0, iso-C16 : 0 and iso-C15 : 0.
30063198	6	38	theme	fatty	602:606	arg1	anteiso-C15 					619:630	anteiso-C15 	619:630	anteiso-C15 	619:630	The major fatty acids were anteiso-C15 : 0, anteiso-C17 : 0, iso-C16 : 0 and iso-C15 : 0.
30063198	3	39	theme	oceani	444:449	arg1	BBH7T					451:455	Brevibacterium oceani BBH7T	429:455	Brevibacterium oceani BBH7T (96.2 %)	429:464	Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that the strain formed a distinct lineage within the genus Brevibacterium and was most closely related to Brevibacterium avium NCFB 3055T (96.3 %), Brevibacterium oceani BBH7T (96.2 %) and Brevibacterium epidermidis NBRC 14811T (96.1 %).
30063198	3	39	theme	oceani	444:449	arg1	%					463:463	96.2 %	458:463	96.2 %	458:463	Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that the strain formed a distinct lineage within the genus Brevibacterium and was most closely related to Brevibacterium avium NCFB 3055T (96.3 %), Brevibacterium oceani BBH7T (96.2 %) and Brevibacterium epidermidis NBRC 14811T (96.1 %).
30063198	3	40	theme	avium	402:406	arg1	3055T					413:417	Brevibacterium avium NCFB 3055T	387:417	Brevibacterium avium NCFB 3055T (96.3 %)	387:426	Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that the strain formed a distinct lineage within the genus Brevibacterium and was most closely related to Brevibacterium avium NCFB 3055T (96.3 %), Brevibacterium oceani BBH7T (96.2 %) and Brevibacterium epidermidis NBRC 14811T (96.1 %).
30063198	3	40	theme	avium	402:406	arg1	%					425:425	96.3 %	420:425	96.3 %	420:425	Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that the strain formed a distinct lineage within the genus Brevibacterium and was most closely related to Brevibacterium avium NCFB 3055T (96.3 %), Brevibacterium oceani BBH7T (96.2 %) and Brevibacterium epidermidis NBRC 14811T (96.1 %).
30063198	7	41	theme	cell-wall	686:694	arg1	peptidoglycan					696:708	The cell-wall peptidoglycan	682:708	The cell-wall peptidoglycan of strain BS05T	682:724	The cell-wall peptidoglycan of strain BS05T contained meso-diaminopimelic acid.
30063198	3	42	theme	gene	247:250	arg1	comparison					261:270	16S rRNA gene sequence comparison	238:270	16S rRNA gene sequence comparison	238:270	Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that the strain formed a distinct lineage within the genus Brevibacterium and was most closely related to Brevibacterium avium NCFB 3055T (96.3 %), Brevibacterium oceani BBH7T (96.2 %) and Brevibacterium epidermidis NBRC 14811T (96.1 %).
30063198	6	43	theme	major	596:600	arg1	acids					608:612	The major fatty acids	592:612	The major fatty acids	592:612	The major fatty acids were anteiso-C15 : 0, anteiso-C17 : 0, iso-C16 : 0 and iso-C15 : 0.
30063198	6	43	theme	major	596:600	arg1	anteiso-C15 					619:630	anteiso-C15 	619:630	anteiso-C15 	619:630	The major fatty acids were anteiso-C15 : 0, anteiso-C17 : 0, iso-C16 : 0 and iso-C15 : 0.
30063198	3	44	theme	NCFB	408:411	arg1	3055T					413:417	Brevibacterium avium NCFB 3055T	387:417	Brevibacterium avium NCFB 3055T (96.3 %)	387:426	Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that the strain formed a distinct lineage within the genus Brevibacterium and was most closely related to Brevibacterium avium NCFB 3055T (96.3 %), Brevibacterium oceani BBH7T (96.2 %) and Brevibacterium epidermidis NBRC 14811T (96.1 %).
30063198	3	44	theme	NCFB	408:411	arg1	%					425:425	96.3 %	420:425	96.3 %	420:425	Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that the strain formed a distinct lineage within the genus Brevibacterium and was most closely related to Brevibacterium avium NCFB 3055T (96.3 %), Brevibacterium oceani BBH7T (96.2 %) and Brevibacterium epidermidis NBRC 14811T (96.1 %).
30063198	3	45	theme	sequence	252:259	arg1	comparison					261:270	16S rRNA gene sequence comparison	238:270	16S rRNA gene sequence comparison	238:270	Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that the strain formed a distinct lineage within the genus Brevibacterium and was most closely related to Brevibacterium avium NCFB 3055T (96.3 %), Brevibacterium oceani BBH7T (96.2 %) and Brevibacterium epidermidis NBRC 14811T (96.1 %).
30063198	11	46	theme	=KACC	1235:1239	arg1	29562T					1252:1257	=KACC 18875T=LMG 29562T	1235:1257	=KACC 18875T=LMG 29562T	1235:1257	nov. is proposed, with the type strain BS05T (=KACC 18875T=LMG 29562T).
30063198	11	46	theme	=KACC	1235:1239	arg1	BS05T					1228:1232	the type strain BS05T	1212:1232	the type strain BS05T (=KACC 18875T=LMG 29562T)	1212:1258	nov. is proposed, with the type strain BS05T (=KACC 18875T=LMG 29562T).
30063198	9	47	theme	Brevibacterium	1046:1059	arg1	species					1025:1031	the recognized species	1010:1031	the recognized species of the genus Brevibacterium	1010:1059	Moreover, the low sequence similarity of the 16S rRNA gene sequencing, physiological, biochemical and chemotaxonomic analyses allowed the phenotypic and genotypic differentiation of strain BS05T from the recognized species of the genus Brevibacterium.
30063198	9	48	theme	gene	864:867	arg1	sequencing					869:878	the 16S rRNA gene sequencing	851:878	the 16S rRNA gene sequencing	851:878	Moreover, the low sequence similarity of the 16S rRNA gene sequencing, physiological, biochemical and chemotaxonomic analyses allowed the phenotypic and genotypic differentiation of strain BS05T from the recognized species of the genus Brevibacterium.
30063198	9	49	theme	chemotaxonomic	912:925	arg1	analyses					927:934	physiological, biochemical and chemotaxonomic analyses	881:934	physiological, biochemical and chemotaxonomic analyses	881:934	Moreover, the low sequence similarity of the 16S rRNA gene sequencing, physiological, biochemical and chemotaxonomic analyses allowed the phenotypic and genotypic differentiation of strain BS05T from the recognized species of the genus Brevibacterium.
30063198	2	50	theme	aerobic	84:90	arg1	bacterium					151:159	A Gram-positive, strictly aerobic, non-motile, milky-white to creamy coloured and rod-shaped bacterium	58:159	A Gram-positive, strictly aerobic, non-motile, milky-white to creamy coloured and rod-shaped bacterium	58:159	A Gram-positive, strictly aerobic, non-motile, milky-white to creamy coloured and rod-shaped bacterium, designated BS05T, was isolated from compost.
30063198	9	51	from	species	1025:1031	arg1	differentiation					973:987	the phenotypic and genotypic differentiation	944:987	the phenotypic and genotypic differentiation of strain BS05T from the recognized species of the genus Brevibacterium	944:1059	Moreover, the low sequence similarity of the 16S rRNA gene sequencing, physiological, biochemical and chemotaxonomic analyses allowed the phenotypic and genotypic differentiation of strain BS05T from the recognized species of the genus Brevibacterium.
30063198	3	52	theme	Brevibacterium	387:400	arg1	3055T					413:417	Brevibacterium avium NCFB 3055T	387:417	Brevibacterium avium NCFB 3055T (96.3 %)	387:426	Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that the strain formed a distinct lineage within the genus Brevibacterium and was most closely related to Brevibacterium avium NCFB 3055T (96.3 %), Brevibacterium oceani BBH7T (96.2 %) and Brevibacterium epidermidis NBRC 14811T (96.1 %).
30063198	3	52	theme	Brevibacterium	387:400	arg1	%					425:425	96.3 %	420:425	96.3 %	420:425	Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that the strain formed a distinct lineage within the genus Brevibacterium and was most closely related to Brevibacterium avium NCFB 3055T (96.3 %), Brevibacterium oceani BBH7T (96.2 %) and Brevibacterium epidermidis NBRC 14811T (96.1 %).
30063198	10	53	theme	Brevibacteriumhankyongi	1161:1183	arg1	sp					1185:1186	the name Brevibacteriumhankyongi sp	1152:1186	the name Brevibacteriumhankyongi sp	1152:1186	Therefore, strain BS05T represents a novel species of the genus Brevibacterium, for which the name Brevibacteriumhankyongi sp.
30063198	10	54	theme	strain	1073:1078	arg1	BS05T					1080:1084	strain BS05T	1073:1084	strain BS05T	1073:1084	Therefore, strain BS05T represents a novel species of the genus Brevibacterium, for which the name Brevibacteriumhankyongi sp.
30063198	10	55	theme	name	1156:1159	arg1	sp					1185:1186	the name Brevibacteriumhankyongi sp	1152:1186	the name Brevibacteriumhankyongi sp	1152:1186	Therefore, strain BS05T represents a novel species of the genus Brevibacterium, for which the name Brevibacteriumhankyongi sp.
30063198	9	56	theme	phenotypic	948:957	arg1	differentiation					973:987	the phenotypic and genotypic differentiation	944:987	the phenotypic and genotypic differentiation of strain BS05T from the recognized species of the genus Brevibacterium	944:1059	Moreover, the low sequence similarity of the 16S rRNA gene sequencing, physiological, biochemical and chemotaxonomic analyses allowed the phenotypic and genotypic differentiation of strain BS05T from the recognized species of the genus Brevibacterium.
30063198	9	57	theme	low	824:826	arg1	similarity					837:846	the low sequence similarity	820:846	the low sequence similarity of the 16S rRNA gene sequencing	820:878	Moreover, the low sequence similarity of the 16S rRNA gene sequencing, physiological, biochemical and chemotaxonomic analyses allowed the phenotypic and genotypic differentiation of strain BS05T from the recognized species of the genus Brevibacterium.
30063198	3	58	theme	Brevibacterium	470:483	arg1	14811T					502:507	Brevibacterium epidermidis NBRC 14811T	470:507	Brevibacterium epidermidis NBRC 14811T (96.1 %)	470:516	Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that the strain formed a distinct lineage within the genus Brevibacterium and was most closely related to Brevibacterium avium NCFB 3055T (96.3 %), Brevibacterium oceani BBH7T (96.2 %) and Brevibacterium epidermidis NBRC 14811T (96.1 %).
30063198	3	58	theme	Brevibacterium	470:483	arg1	%					515:515	96.1 %	510:515	96.1 %	510:515	Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that the strain formed a distinct lineage within the genus Brevibacterium and was most closely related to Brevibacterium avium NCFB 3055T (96.3 %), Brevibacterium oceani BBH7T (96.2 %) and Brevibacterium epidermidis NBRC 14811T (96.1 %).
30063198	9	59	theme	sequencing	869:878	arg1	similarity					837:846	the low sequence similarity	820:846	the low sequence similarity of the 16S rRNA gene sequencing	820:878	Moreover, the low sequence similarity of the 16S rRNA gene sequencing, physiological, biochemical and chemotaxonomic analyses allowed the phenotypic and genotypic differentiation of strain BS05T from the recognized species of the genus Brevibacterium.
30063198	9	60	theme	sequence	828:835	arg1	similarity					837:846	the low sequence similarity	820:846	the low sequence similarity of the 16S rRNA gene sequencing	820:878	Moreover, the low sequence similarity of the 16S rRNA gene sequencing, physiological, biochemical and chemotaxonomic analyses allowed the phenotypic and genotypic differentiation of strain BS05T from the recognized species of the genus Brevibacterium.
30063198	3	61	theme	epidermidis	485:495	arg1	14811T					502:507	Brevibacterium epidermidis NBRC 14811T	470:507	Brevibacterium epidermidis NBRC 14811T (96.1 %)	470:516	Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that the strain formed a distinct lineage within the genus Brevibacterium and was most closely related to Brevibacterium avium NCFB 3055T (96.3 %), Brevibacterium oceani BBH7T (96.2 %) and Brevibacterium epidermidis NBRC 14811T (96.1 %).
30063198	3	61	theme	epidermidis	485:495	arg1	%					515:515	96.1 %	510:515	96.1 %	510:515	Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that the strain formed a distinct lineage within the genus Brevibacterium and was most closely related to Brevibacterium avium NCFB 3055T (96.3 %), Brevibacterium oceani BBH7T (96.2 %) and Brevibacterium epidermidis NBRC 14811T (96.1 %).
30063198	9	62	theme	genotypic	963:971	arg1	differentiation					973:987	the phenotypic and genotypic differentiation	944:987	the phenotypic and genotypic differentiation of strain BS05T from the recognized species of the genus Brevibacterium	944:1059	Moreover, the low sequence similarity of the 16S rRNA gene sequencing, physiological, biochemical and chemotaxonomic analyses allowed the phenotypic and genotypic differentiation of strain BS05T from the recognized species of the genus Brevibacterium.
30063198	10	63	theme	novel	1099:1103	arg1	species					1105:1111	a novel species	1097:1111	a novel species	1097:1111	Therefore, strain BS05T represents a novel species of the genus Brevibacterium, for which the name Brevibacteriumhankyongi sp.
31281995	6	0	theme	screening	937:945	arg1	design					947:952	an initial fractional factorial screening design	905:952	an initial fractional factorial screening design	905:952	Method optimization was carried out using an experimental design approach, performing an initial fractional factorial screening design, followed by a central composite design to establish the optimal analytical conditions.
31281995	5	1	theme	selector	668:675	arg1	system					677:682	A dual selector system	661:682	A dual selector system containing sulfobutyl-ether-β-CD degree of substitution 6.5 and native γ-CD	661:758	A dual selector system containing sulfobutyl-ether-β-CD degree of substitution 6.5 and native γ-CD proved to be the most adequate system for the separations.
31281995	5	1	theme	selector	668:675	arg1	system					791:796	the most adequate system	773:796	the most adequate system for the separations	773:816	A dual selector system containing sulfobutyl-ether-β-CD degree of substitution 6.5 and native γ-CD proved to be the most adequate system for the separations.
31281995	7	2	theme	=	1472:1472	arg1	enantiomers					1480:1490	rabeprazole (Rs = 2.53) enantiomers	1456:1490	rabeprazole (Rs = 2.53) enantiomers with favorable migration order (in both cases the S-enantiomers migrates first)	1456:1570	The optimized methods (25 mM phosphate buffer, pH 7, 10 mM sulfobutyl-ether-β-CD/20 mM γ-CD, +20 kV voltage; 17°C temperature; 50 mbar/3 s injection, detection at 210 nm for lansoprazole; 25 mM phosphate buffer, pH 7, 15 mM sulfobutyl-ether-β-CD/30 mM γ-CD, +20 kV voltage; 18°C temperature; 50 mbar/3 s injection, detection at 210 nm for rabeprazole) provided baseline separation for lansoprazole (Rs = 2.91) and rabeprazole (Rs = 2.53) enantiomers with favorable migration order (in both cases the S-enantiomers migrates first).
31281995	7	2	theme	=	1472:1472	arg1	Rs					1469:1470	Rs = 2.53	1469:1477	Rs = 2.53	1469:1477	The optimized methods (25 mM phosphate buffer, pH 7, 10 mM sulfobutyl-ether-β-CD/20 mM γ-CD, +20 kV voltage; 17°C temperature; 50 mbar/3 s injection, detection at 210 nm for lansoprazole; 25 mM phosphate buffer, pH 7, 15 mM sulfobutyl-ether-β-CD/30 mM γ-CD, +20 kV voltage; 18°C temperature; 50 mbar/3 s injection, detection at 210 nm for rabeprazole) provided baseline separation for lansoprazole (Rs = 2.91) and rabeprazole (Rs = 2.53) enantiomers with favorable migration order (in both cases the S-enantiomers migrates first).
31281995	3	3	theme	suitable	500:507	arg1	Sulfobutyl-ether-β-CD					398:418	Sulfobutyl-ether-β-CD	398:418	Sulfobutyl-ether-β-CD with a degree of substitution of 6.5 and 10 at neutral pH	398:476	Sulfobutyl-ether-β-CD with a degree of substitution of 6.5 and 10 at neutral pH proved to be the most suitable chiral selector for both compounds.
31281995	3	3	theme	suitable	500:507	arg1	selector					516:523	the most suitable chiral selector	491:523	the most suitable chiral selector for both compounds	491:542	Sulfobutyl-ether-β-CD with a degree of substitution of 6.5 and 10 at neutral pH proved to be the most suitable chiral selector for both compounds.
31281995	1	4	theme	proton	274:279	arg1	rabeprazole					257:267	rabeprazole	257:267	rabeprazole	257:267	Novel capillary electrophoresis methods using CDs as chiral selectors were developed and validated for the chiral separation of lansoprazole and rabeprazole, two proton pump inhibitors.
31281995	1	4	theme	proton	274:279	arg1	inhibitors					286:295	two proton pump inhibitors	270:295	two proton pump inhibitors	270:295	Novel capillary electrophoresis methods using CDs as chiral selectors were developed and validated for the chiral separation of lansoprazole and rabeprazole, two proton pump inhibitors.
31281995	6	5	theme	fractional	916:925	arg1	design					947:952	an initial fractional factorial screening design	905:952	an initial fractional factorial screening design	905:952	Method optimization was carried out using an experimental design approach, performing an initial fractional factorial screening design, followed by a central composite design to establish the optimal analytical conditions.
31281995	5	6	theme	dual	663:666	arg1	system					677:682	A dual selector system	661:682	A dual selector system containing sulfobutyl-ether-β-CD degree of substitution 6.5 and native γ-CD	661:758	A dual selector system containing sulfobutyl-ether-β-CD degree of substitution 6.5 and native γ-CD proved to be the most adequate system for the separations.
31281995	5	6	theme	dual	663:666	arg1	system					791:796	the most adequate system	773:796	the most adequate system for the separations	773:816	A dual selector system containing sulfobutyl-ether-β-CD degree of substitution 6.5 and native γ-CD proved to be the most adequate system for the separations.
31281995	2	7	theme	different	307:315	arg1	CDs					337:339	Fourteen different neutral and anionic CDs	298:339	Fourteen different neutral and anionic CDs	298:339	Fourteen different neutral and anionic CDs were screened at pH 4 and 7 in the preliminary analysis.
31281995	7	8	with	enantiomers	1480:1490	arg1	order					1517:1521	favorable migration order	1497:1521	favorable migration order (in both cases the S-enantiomers migrates first)	1497:1570	The optimized methods (25 mM phosphate buffer, pH 7, 10 mM sulfobutyl-ether-β-CD/20 mM γ-CD, +20 kV voltage; 17°C temperature; 50 mbar/3 s injection, detection at 210 nm for lansoprazole; 25 mM phosphate buffer, pH 7, 15 mM sulfobutyl-ether-β-CD/30 mM γ-CD, +20 kV voltage; 18°C temperature; 50 mbar/3 s injection, detection at 210 nm for rabeprazole) provided baseline separation for lansoprazole (Rs = 2.91) and rabeprazole (Rs = 2.53) enantiomers with favorable migration order (in both cases the S-enantiomers migrates first).
31281995	7	9	theme	baseline	1403:1410	arg1	separation					1412:1421	baseline separation	1403:1421	baseline separation for lansoprazole (Rs = 2.91) and rabeprazole (Rs = 2.53) enantiomers with favorable migration order (in both cases the S-enantiomers migrates first)	1403:1570	The optimized methods (25 mM phosphate buffer, pH 7, 10 mM sulfobutyl-ether-β-CD/20 mM γ-CD, +20 kV voltage; 17°C temperature; 50 mbar/3 s injection, detection at 210 nm for lansoprazole; 25 mM phosphate buffer, pH 7, 15 mM sulfobutyl-ether-β-CD/30 mM γ-CD, +20 kV voltage; 18°C temperature; 50 mbar/3 s injection, detection at 210 nm for rabeprazole) provided baseline separation for lansoprazole (Rs = 2.91) and rabeprazole (Rs = 2.53) enantiomers with favorable migration order (in both cases the S-enantiomers migrates first).
31281995	6	10	theme	factorial	927:935	arg1	design					947:952	an initial fractional factorial screening design	905:952	an initial fractional factorial screening design	905:952	Method optimization was carried out using an experimental design approach, performing an initial fractional factorial screening design, followed by a central composite design to establish the optimal analytical conditions.
31281995	7	11	theme	10 mM	1095:1099	arg1	γ-CD					1129:1132	10 mM sulfobutyl-ether-β-CD/20 mM γ-CD	1095:1132	10 mM sulfobutyl-ether-β-CD/20 mM γ-CD	1095:1132	The optimized methods (25 mM phosphate buffer, pH 7, 10 mM sulfobutyl-ether-β-CD/20 mM γ-CD, +20 kV voltage; 17°C temperature; 50 mbar/3 s injection, detection at 210 nm for lansoprazole; 25 mM phosphate buffer, pH 7, 15 mM sulfobutyl-ether-β-CD/30 mM γ-CD, +20 kV voltage; 18°C temperature; 50 mbar/3 s injection, detection at 210 nm for rabeprazole) provided baseline separation for lansoprazole (Rs = 2.91) and rabeprazole (Rs = 2.53) enantiomers with favorable migration order (in both cases the S-enantiomers migrates first).
31281995	7	11	theme	10 mM	1095:1099	arg1	pH					1089:1090	pH 7	1089:1092	pH 7	1089:1092	The optimized methods (25 mM phosphate buffer, pH 7, 10 mM sulfobutyl-ether-β-CD/20 mM γ-CD, +20 kV voltage; 17°C temperature; 50 mbar/3 s injection, detection at 210 nm for lansoprazole; 25 mM phosphate buffer, pH 7, 15 mM sulfobutyl-ether-β-CD/30 mM γ-CD, +20 kV voltage; 18°C temperature; 50 mbar/3 s injection, detection at 210 nm for rabeprazole) provided baseline separation for lansoprazole (Rs = 2.91) and rabeprazole (Rs = 2.53) enantiomers with favorable migration order (in both cases the S-enantiomers migrates first).
31281995	4	12	theme	enantiomer	620:629	arg1	separation					631:640	enantiomer separation	620:640	enantiomer separation	620:640	Various dual CD systems were also compared, and the possible mechanisms of enantiomer separation were investigated.
31281995	1	13	theme	chiral	219:224	arg1	separation					226:235	the chiral separation	215:235	the chiral separation of lansoprazole and rabeprazole, two proton pump inhibitors	215:295	Novel capillary electrophoresis methods using CDs as chiral selectors were developed and validated for the chiral separation of lansoprazole and rabeprazole, two proton pump inhibitors.
31281995	6	14	theme	central	969:975	arg1	design					987:992	a central composite design	967:992	a central composite design	967:992	Method optimization was carried out using an experimental design approach, performing an initial fractional factorial screening design, followed by a central composite design to establish the optimal analytical conditions.
31281995	7	15	theme	mbar/3	1172:1177	arg1	injection					1181:1189	50 mbar/3 s injection	1169:1189	50 mbar/3 s injection	1169:1189	The optimized methods (25 mM phosphate buffer, pH 7, 10 mM sulfobutyl-ether-β-CD/20 mM γ-CD, +20 kV voltage; 17°C temperature; 50 mbar/3 s injection, detection at 210 nm for lansoprazole; 25 mM phosphate buffer, pH 7, 15 mM sulfobutyl-ether-β-CD/30 mM γ-CD, +20 kV voltage; 18°C temperature; 50 mbar/3 s injection, detection at 210 nm for rabeprazole) provided baseline separation for lansoprazole (Rs = 2.91) and rabeprazole (Rs = 2.53) enantiomers with favorable migration order (in both cases the S-enantiomers migrates first).
31281995	7	16	theme	phosphate	1071:1079	arg1	buffer					1081:1086	25 mM phosphate buffer	1065:1086	25 mM phosphate buffer	1065:1086	The optimized methods (25 mM phosphate buffer, pH 7, 10 mM sulfobutyl-ether-β-CD/20 mM γ-CD, +20 kV voltage; 17°C temperature; 50 mbar/3 s injection, detection at 210 nm for lansoprazole; 25 mM phosphate buffer, pH 7, 15 mM sulfobutyl-ether-β-CD/30 mM γ-CD, +20 kV voltage; 18°C temperature; 50 mbar/3 s injection, detection at 210 nm for rabeprazole) provided baseline separation for lansoprazole (Rs = 2.91) and rabeprazole (Rs = 2.53) enantiomers with favorable migration order (in both cases the S-enantiomers migrates first).
31281995	8	17	theme	%	1728:1728	arg1	distomer					1730:1737	0.15% distomer	1724:1737	0.15% distomer	1724:1737	The optimized methods were validated according to current guidelines and proved to be reliable, linear, precise, and accurate for the determination of 0.15% distomer as chiral impurity in dexlansoprazole and dexrabeprazole samples.
31281995	1	18	theme	Novel	112:116	arg1	methods					144:150	Novel capillary electrophoresis methods	112:150	Novel capillary electrophoresis methods using CDs as chiral selectors	112:180	Novel capillary electrophoresis methods using CDs as chiral selectors were developed and validated for the chiral separation of lansoprazole and rabeprazole, two proton pump inhibitors.
31281995	5	19	theme	sulfobutyl-ether-β-CD	695:715	arg1	degree					717:722	sulfobutyl-ether-β-CD degree	695:722	sulfobutyl-ether-β-CD degree of substitution 6.5 and native γ-CD	695:758	A dual selector system containing sulfobutyl-ether-β-CD degree of substitution 6.5 and native γ-CD proved to be the most adequate system for the separations.
31281995	7	20	theme	s	1344:1344	arg1	injection					1346:1354	50 mbar/3 s injection	1334:1354	50 mbar/3 s injection	1334:1354	The optimized methods (25 mM phosphate buffer, pH 7, 10 mM sulfobutyl-ether-β-CD/20 mM γ-CD, +20 kV voltage; 17°C temperature; 50 mbar/3 s injection, detection at 210 nm for lansoprazole; 25 mM phosphate buffer, pH 7, 15 mM sulfobutyl-ether-β-CD/30 mM γ-CD, +20 kV voltage; 18°C temperature; 50 mbar/3 s injection, detection at 210 nm for rabeprazole) provided baseline separation for lansoprazole (Rs = 2.91) and rabeprazole (Rs = 2.53) enantiomers with favorable migration order (in both cases the S-enantiomers migrates first).
31281995	7	21	theme	phosphate	1236:1244	arg1	pH					1254:1255	pH 7	1254:1257	pH 7	1254:1257	The optimized methods (25 mM phosphate buffer, pH 7, 10 mM sulfobutyl-ether-β-CD/20 mM γ-CD, +20 kV voltage; 17°C temperature; 50 mbar/3 s injection, detection at 210 nm for lansoprazole; 25 mM phosphate buffer, pH 7, 15 mM sulfobutyl-ether-β-CD/30 mM γ-CD, +20 kV voltage; 18°C temperature; 50 mbar/3 s injection, detection at 210 nm for rabeprazole) provided baseline separation for lansoprazole (Rs = 2.91) and rabeprazole (Rs = 2.53) enantiomers with favorable migration order (in both cases the S-enantiomers migrates first).
31281995	7	21	theme	phosphate	1236:1244	arg1	buffer					1246:1251	25 mM phosphate buffer	1230:1251	25 mM phosphate buffer	1230:1251	The optimized methods (25 mM phosphate buffer, pH 7, 10 mM sulfobutyl-ether-β-CD/20 mM γ-CD, +20 kV voltage; 17°C temperature; 50 mbar/3 s injection, detection at 210 nm for lansoprazole; 25 mM phosphate buffer, pH 7, 15 mM sulfobutyl-ether-β-CD/30 mM γ-CD, +20 kV voltage; 18°C temperature; 50 mbar/3 s injection, detection at 210 nm for rabeprazole) provided baseline separation for lansoprazole (Rs = 2.91) and rabeprazole (Rs = 2.53) enantiomers with favorable migration order (in both cases the S-enantiomers migrates first).
31281995	7	22	dep	provided	1394:1401	arg1	buffer					1081:1086	25 mM phosphate buffer	1065:1086	25 mM phosphate buffer	1065:1086	The optimized methods (25 mM phosphate buffer, pH 7, 10 mM sulfobutyl-ether-β-CD/20 mM γ-CD, +20 kV voltage; 17°C temperature; 50 mbar/3 s injection, detection at 210 nm for lansoprazole; 25 mM phosphate buffer, pH 7, 15 mM sulfobutyl-ether-β-CD/30 mM γ-CD, +20 kV voltage; 18°C temperature; 50 mbar/3 s injection, detection at 210 nm for rabeprazole) provided baseline separation for lansoprazole (Rs = 2.91) and rabeprazole (Rs = 2.53) enantiomers with favorable migration order (in both cases the S-enantiomers migrates first).
31281995	0	23	theme	dual	85:88	arg1	systems					103:109	dual cyclodextrin systems	85:109	dual cyclodextrin systems	85:109	Chiral separation of lansoprazole and rabeprazole by capillary electrophoresis using dual cyclodextrin systems.
31281995	3	24	from	degree	427:432	arg1	pH					475:476	neutral pH	467:476	neutral pH	467:476	Sulfobutyl-ether-β-CD with a degree of substitution of 6.5 and 10 at neutral pH proved to be the most suitable chiral selector for both compounds.
31281995	8	25	theme	dexlansoprazole	1761:1775	arg1	samples					1796:1802	dexlansoprazole and dexrabeprazole samples	1761:1802	dexlansoprazole and dexrabeprazole samples	1761:1802	The optimized methods were validated according to current guidelines and proved to be reliable, linear, precise, and accurate for the determination of 0.15% distomer as chiral impurity in dexlansoprazole and dexrabeprazole samples.
31281995	7	26	theme	+20 kV	1135:1140	arg1	pH					1089:1090	pH 7	1089:1092	pH 7	1089:1092	The optimized methods (25 mM phosphate buffer, pH 7, 10 mM sulfobutyl-ether-β-CD/20 mM γ-CD, +20 kV voltage; 17°C temperature; 50 mbar/3 s injection, detection at 210 nm for lansoprazole; 25 mM phosphate buffer, pH 7, 15 mM sulfobutyl-ether-β-CD/30 mM γ-CD, +20 kV voltage; 18°C temperature; 50 mbar/3 s injection, detection at 210 nm for rabeprazole) provided baseline separation for lansoprazole (Rs = 2.91) and rabeprazole (Rs = 2.53) enantiomers with favorable migration order (in both cases the S-enantiomers migrates first).
31281995	7	26	theme	+20 kV	1135:1140	arg1	voltage					1142:1148	+20 kV voltage	1135:1148	+20 kV voltage	1135:1148	The optimized methods (25 mM phosphate buffer, pH 7, 10 mM sulfobutyl-ether-β-CD/20 mM γ-CD, +20 kV voltage; 17°C temperature; 50 mbar/3 s injection, detection at 210 nm for lansoprazole; 25 mM phosphate buffer, pH 7, 15 mM sulfobutyl-ether-β-CD/30 mM γ-CD, +20 kV voltage; 18°C temperature; 50 mbar/3 s injection, detection at 210 nm for rabeprazole) provided baseline separation for lansoprazole (Rs = 2.91) and rabeprazole (Rs = 2.53) enantiomers with favorable migration order (in both cases the S-enantiomers migrates first).
31281995	6	27	theme	optimal	1011:1017	arg1	conditions					1030:1039	the optimal analytical conditions	1007:1039	the optimal analytical conditions	1007:1039	Method optimization was carried out using an experimental design approach, performing an initial fractional factorial screening design, followed by a central composite design to establish the optimal analytical conditions.
31281995	5	28	theme	native	748:753	arg1	γ-CD					755:758	native γ-CD	748:758	native γ-CD	748:758	A dual selector system containing sulfobutyl-ether-β-CD degree of substitution 6.5 and native γ-CD proved to be the most adequate system for the separations.
31281995	8	29	theme	dexrabeprazole	1781:1794	arg1	samples					1796:1802	dexlansoprazole and dexrabeprazole samples	1761:1802	dexlansoprazole and dexrabeprazole samples	1761:1802	The optimized methods were validated according to current guidelines and proved to be reliable, linear, precise, and accurate for the determination of 0.15% distomer as chiral impurity in dexlansoprazole and dexrabeprazole samples.
31281995	3	30	theme	6.5	453:455	arg1	substitution					437:448	substitution	437:448	substitution of 6.5 and 10 at neutral pH	437:476	Sulfobutyl-ether-β-CD with a degree of substitution of 6.5 and 10 at neutral pH proved to be the most suitable chiral selector for both compounds.
31281995	0	31	theme	rabeprazole	38:48	arg1	separation					7:16	Chiral separation	0:16	Chiral separation of lansoprazole and rabeprazole by capillary electrophoresis	0:77	Chiral separation of lansoprazole and rabeprazole by capillary electrophoresis using dual cyclodextrin systems.
31281995	7	32	theme	50 mbar/3	1334:1342	arg1	injection					1346:1354	50 mbar/3 s injection	1334:1354	50 mbar/3 s injection	1334:1354	The optimized methods (25 mM phosphate buffer, pH 7, 10 mM sulfobutyl-ether-β-CD/20 mM γ-CD, +20 kV voltage; 17°C temperature; 50 mbar/3 s injection, detection at 210 nm for lansoprazole; 25 mM phosphate buffer, pH 7, 15 mM sulfobutyl-ether-β-CD/30 mM γ-CD, +20 kV voltage; 18°C temperature; 50 mbar/3 s injection, detection at 210 nm for rabeprazole) provided baseline separation for lansoprazole (Rs = 2.91) and rabeprazole (Rs = 2.53) enantiomers with favorable migration order (in both cases the S-enantiomers migrates first).
31281995	3	33	theme	substitution	437:448	arg1	degree					427:432	a degree	425:432	a degree of substitution of 6.5 and 10 at neutral pH	425:476	Sulfobutyl-ether-β-CD with a degree of substitution of 6.5 and 10 at neutral pH proved to be the most suitable chiral selector for both compounds.
31281995	0	34	theme	Chiral	0:5	arg1	separation					7:16	Chiral separation	0:16	Chiral separation of lansoprazole and rabeprazole by capillary electrophoresis	0:77	Chiral separation of lansoprazole and rabeprazole by capillary electrophoresis using dual cyclodextrin systems.
31281995	4	35	theme	Various	545:551	arg1	systems					561:567	Various dual CD systems	545:567	Various dual CD systems	545:567	Various dual CD systems were also compared, and the possible mechanisms of enantiomer separation were investigated.
31281995	3	36	theme	neutral	467:473	arg1	pH					475:476	neutral pH	467:476	neutral pH	467:476	Sulfobutyl-ether-β-CD with a degree of substitution of 6.5 and 10 at neutral pH proved to be the most suitable chiral selector for both compounds.
31281995	7	37	theme	s	1179:1179	arg1	injection					1181:1189	50 mbar/3 s injection	1169:1189	50 mbar/3 s injection	1169:1189	The optimized methods (25 mM phosphate buffer, pH 7, 10 mM sulfobutyl-ether-β-CD/20 mM γ-CD, +20 kV voltage; 17°C temperature; 50 mbar/3 s injection, detection at 210 nm for lansoprazole; 25 mM phosphate buffer, pH 7, 15 mM sulfobutyl-ether-β-CD/30 mM γ-CD, +20 kV voltage; 18°C temperature; 50 mbar/3 s injection, detection at 210 nm for rabeprazole) provided baseline separation for lansoprazole (Rs = 2.91) and rabeprazole (Rs = 2.53) enantiomers with favorable migration order (in both cases the S-enantiomers migrates first).
31281995	1	38	theme	capillary	118:126	arg1	methods					144:150	Novel capillary electrophoresis methods	112:150	Novel capillary electrophoresis methods using CDs as chiral selectors	112:180	Novel capillary electrophoresis methods using CDs as chiral selectors were developed and validated for the chiral separation of lansoprazole and rabeprazole, two proton pump inhibitors.
31281995	4	39	theme	CD	558:559	arg1	systems					561:567	Various dual CD systems	545:567	Various dual CD systems	545:567	Various dual CD systems were also compared, and the possible mechanisms of enantiomer separation were investigated.
31281995	3	40	theme	10	461:462	arg1	substitution					437:448	substitution	437:448	substitution of 6.5 and 10 at neutral pH	437:476	Sulfobutyl-ether-β-CD with a degree of substitution of 6.5 and 10 at neutral pH proved to be the most suitable chiral selector for both compounds.
31281995	7	41	with	lansoprazole	1427:1438	arg1	order					1517:1521	favorable migration order	1497:1521	favorable migration order (in both cases the S-enantiomers migrates first)	1497:1570	The optimized methods (25 mM phosphate buffer, pH 7, 10 mM sulfobutyl-ether-β-CD/20 mM γ-CD, +20 kV voltage; 17°C temperature; 50 mbar/3 s injection, detection at 210 nm for lansoprazole; 25 mM phosphate buffer, pH 7, 15 mM sulfobutyl-ether-β-CD/30 mM γ-CD, +20 kV voltage; 18°C temperature; 50 mbar/3 s injection, detection at 210 nm for rabeprazole) provided baseline separation for lansoprazole (Rs = 2.91) and rabeprazole (Rs = 2.53) enantiomers with favorable migration order (in both cases the S-enantiomers migrates first).
31281995	6	42	theme	experimental	864:875	arg1	approach					884:891	an experimental design approach	861:891	an experimental design approach	861:891	Method optimization was carried out using an experimental design approach, performing an initial fractional factorial screening design, followed by a central composite design to establish the optimal analytical conditions.
31281995	7	43	theme	+20 kV	1300:1305	arg1	voltage					1307:1313	+20 kV voltage	1300:1313	+20 kV voltage	1300:1313	The optimized methods (25 mM phosphate buffer, pH 7, 10 mM sulfobutyl-ether-β-CD/20 mM γ-CD, +20 kV voltage; 17°C temperature; 50 mbar/3 s injection, detection at 210 nm for lansoprazole; 25 mM phosphate buffer, pH 7, 15 mM sulfobutyl-ether-β-CD/30 mM γ-CD, +20 kV voltage; 18°C temperature; 50 mbar/3 s injection, detection at 210 nm for rabeprazole) provided baseline separation for lansoprazole (Rs = 2.91) and rabeprazole (Rs = 2.53) enantiomers with favorable migration order (in both cases the S-enantiomers migrates first).
31281995	7	43	theme	+20 kV	1300:1305	arg1	pH					1254:1255	pH 7	1254:1257	pH 7	1254:1257	The optimized methods (25 mM phosphate buffer, pH 7, 10 mM sulfobutyl-ether-β-CD/20 mM γ-CD, +20 kV voltage; 17°C temperature; 50 mbar/3 s injection, detection at 210 nm for lansoprazole; 25 mM phosphate buffer, pH 7, 15 mM sulfobutyl-ether-β-CD/30 mM γ-CD, +20 kV voltage; 18°C temperature; 50 mbar/3 s injection, detection at 210 nm for rabeprazole) provided baseline separation for lansoprazole (Rs = 2.91) and rabeprazole (Rs = 2.53) enantiomers with favorable migration order (in both cases the S-enantiomers migrates first).
31281995	5	44	contain	containing	684:693	arg1	system					791:796	the most adequate system	773:796	the most adequate system for the separations	773:816	A dual selector system containing sulfobutyl-ether-β-CD degree of substitution 6.5 and native γ-CD proved to be the most adequate system for the separations.
31281995	5	44	contain	containing	684:693	arg1	system					677:682	A dual selector system	661:682	A dual selector system containing sulfobutyl-ether-β-CD degree of substitution 6.5 and native γ-CD	661:758	A dual selector system containing sulfobutyl-ether-β-CD degree of substitution 6.5 and native γ-CD proved to be the most adequate system for the separations.
31281995	5	44	contain	containing	684:693	arg2	degree					717:722	sulfobutyl-ether-β-CD degree	695:722	sulfobutyl-ether-β-CD degree of substitution 6.5 and native γ-CD	695:758	A dual selector system containing sulfobutyl-ether-β-CD degree of substitution 6.5 and native γ-CD proved to be the most adequate system for the separations.
31281995	7	45	theme	favorable	1497:1505	arg1	order					1517:1521	favorable migration order	1497:1521	favorable migration order (in both cases the S-enantiomers migrates first)	1497:1570	The optimized methods (25 mM phosphate buffer, pH 7, 10 mM sulfobutyl-ether-β-CD/20 mM γ-CD, +20 kV voltage; 17°C temperature; 50 mbar/3 s injection, detection at 210 nm for lansoprazole; 25 mM phosphate buffer, pH 7, 15 mM sulfobutyl-ether-β-CD/30 mM γ-CD, +20 kV voltage; 18°C temperature; 50 mbar/3 s injection, detection at 210 nm for rabeprazole) provided baseline separation for lansoprazole (Rs = 2.91) and rabeprazole (Rs = 2.53) enantiomers with favorable migration order (in both cases the S-enantiomers migrates first).
31281995	3	46	from	pH	475:476	arg1	substitution					437:448	substitution	437:448	substitution of 6.5 and 10 at neutral pH	437:476	Sulfobutyl-ether-β-CD with a degree of substitution of 6.5 and 10 at neutral pH proved to be the most suitable chiral selector for both compounds.
31281995	3	46	from	pH	475:476	arg1	degree					427:432	a degree	425:432	a degree of substitution of 6.5 and 10 at neutral pH	425:476	Sulfobutyl-ether-β-CD with a degree of substitution of 6.5 and 10 at neutral pH proved to be the most suitable chiral selector for both compounds.
31281995	8	47	theme	current	1623:1629	arg1	guidelines					1631:1640	current guidelines	1623:1640	current guidelines	1623:1640	The optimized methods were validated according to current guidelines and proved to be reliable, linear, precise, and accurate for the determination of 0.15% distomer as chiral impurity in dexlansoprazole and dexrabeprazole samples.
31281995	1	48	theme	lansoprazole	240:251	arg1	separation					226:235	the chiral separation	215:235	the chiral separation of lansoprazole and rabeprazole, two proton pump inhibitors	215:295	Novel capillary electrophoresis methods using CDs as chiral selectors were developed and validated for the chiral separation of lansoprazole and rabeprazole, two proton pump inhibitors.
31281995	8	49	from	impurity	1749:1756	arg1	samples					1796:1802	dexlansoprazole and dexrabeprazole samples	1761:1802	dexlansoprazole and dexrabeprazole samples	1761:1802	The optimized methods were validated according to current guidelines and proved to be reliable, linear, precise, and accurate for the determination of 0.15% distomer as chiral impurity in dexlansoprazole and dexrabeprazole samples.
31281995	7	50	theme	15 mM	1260:1264	arg1	pH					1254:1255	pH 7	1254:1257	pH 7	1254:1257	The optimized methods (25 mM phosphate buffer, pH 7, 10 mM sulfobutyl-ether-β-CD/20 mM γ-CD, +20 kV voltage; 17°C temperature; 50 mbar/3 s injection, detection at 210 nm for lansoprazole; 25 mM phosphate buffer, pH 7, 15 mM sulfobutyl-ether-β-CD/30 mM γ-CD, +20 kV voltage; 18°C temperature; 50 mbar/3 s injection, detection at 210 nm for rabeprazole) provided baseline separation for lansoprazole (Rs = 2.91) and rabeprazole (Rs = 2.53) enantiomers with favorable migration order (in both cases the S-enantiomers migrates first).
31281995	7	50	theme	15 mM	1260:1264	arg1	γ-CD					1294:1297	15 mM sulfobutyl-ether-β-CD/30 mM γ-CD	1260:1297	15 mM sulfobutyl-ether-β-CD/30 mM γ-CD	1260:1297	The optimized methods (25 mM phosphate buffer, pH 7, 10 mM sulfobutyl-ether-β-CD/20 mM γ-CD, +20 kV voltage; 17°C temperature; 50 mbar/3 s injection, detection at 210 nm for lansoprazole; 25 mM phosphate buffer, pH 7, 15 mM sulfobutyl-ether-β-CD/30 mM γ-CD, +20 kV voltage; 18°C temperature; 50 mbar/3 s injection, detection at 210 nm for rabeprazole) provided baseline separation for lansoprazole (Rs = 2.91) and rabeprazole (Rs = 2.53) enantiomers with favorable migration order (in both cases the S-enantiomers migrates first).
31281995	7	51	dep	buffer	1081:1086	arg1	buffer					1246:1251	25 mM phosphate buffer	1230:1251	25 mM phosphate buffer	1230:1251	The optimized methods (25 mM phosphate buffer, pH 7, 10 mM sulfobutyl-ether-β-CD/20 mM γ-CD, +20 kV voltage; 17°C temperature; 50 mbar/3 s injection, detection at 210 nm for lansoprazole; 25 mM phosphate buffer, pH 7, 15 mM sulfobutyl-ether-β-CD/30 mM γ-CD, +20 kV voltage; 18°C temperature; 50 mbar/3 s injection, detection at 210 nm for rabeprazole) provided baseline separation for lansoprazole (Rs = 2.91) and rabeprazole (Rs = 2.53) enantiomers with favorable migration order (in both cases the S-enantiomers migrates first).
31281995	7	51	dep	buffer	1081:1086	arg1	voltage					1142:1148	+20 kV voltage	1135:1148	+20 kV voltage	1135:1148	The optimized methods (25 mM phosphate buffer, pH 7, 10 mM sulfobutyl-ether-β-CD/20 mM γ-CD, +20 kV voltage; 17°C temperature; 50 mbar/3 s injection, detection at 210 nm for lansoprazole; 25 mM phosphate buffer, pH 7, 15 mM sulfobutyl-ether-β-CD/30 mM γ-CD, +20 kV voltage; 18°C temperature; 50 mbar/3 s injection, detection at 210 nm for rabeprazole) provided baseline separation for lansoprazole (Rs = 2.91) and rabeprazole (Rs = 2.53) enantiomers with favorable migration order (in both cases the S-enantiomers migrates first).
31281995	7	51	dep	buffer	1081:1086	arg1	pH					1254:1255	pH 7	1254:1257	pH 7	1254:1257	The optimized methods (25 mM phosphate buffer, pH 7, 10 mM sulfobutyl-ether-β-CD/20 mM γ-CD, +20 kV voltage; 17°C temperature; 50 mbar/3 s injection, detection at 210 nm for lansoprazole; 25 mM phosphate buffer, pH 7, 15 mM sulfobutyl-ether-β-CD/30 mM γ-CD, +20 kV voltage; 18°C temperature; 50 mbar/3 s injection, detection at 210 nm for rabeprazole) provided baseline separation for lansoprazole (Rs = 2.91) and rabeprazole (Rs = 2.53) enantiomers with favorable migration order (in both cases the S-enantiomers migrates first).
31281995	7	51	dep	buffer	1081:1086	arg1	injection					1181:1189	50 mbar/3 s injection	1169:1189	50 mbar/3 s injection	1169:1189	The optimized methods (25 mM phosphate buffer, pH 7, 10 mM sulfobutyl-ether-β-CD/20 mM γ-CD, +20 kV voltage; 17°C temperature; 50 mbar/3 s injection, detection at 210 nm for lansoprazole; 25 mM phosphate buffer, pH 7, 15 mM sulfobutyl-ether-β-CD/30 mM γ-CD, +20 kV voltage; 18°C temperature; 50 mbar/3 s injection, detection at 210 nm for rabeprazole) provided baseline separation for lansoprazole (Rs = 2.91) and rabeprazole (Rs = 2.53) enantiomers with favorable migration order (in both cases the S-enantiomers migrates first).
31281995	7	51	dep	buffer	1081:1086	arg1	γ-CD					1129:1132	10 mM sulfobutyl-ether-β-CD/20 mM γ-CD	1095:1132	10 mM sulfobutyl-ether-β-CD/20 mM γ-CD	1095:1132	The optimized methods (25 mM phosphate buffer, pH 7, 10 mM sulfobutyl-ether-β-CD/20 mM γ-CD, +20 kV voltage; 17°C temperature; 50 mbar/3 s injection, detection at 210 nm for lansoprazole; 25 mM phosphate buffer, pH 7, 15 mM sulfobutyl-ether-β-CD/30 mM γ-CD, +20 kV voltage; 18°C temperature; 50 mbar/3 s injection, detection at 210 nm for rabeprazole) provided baseline separation for lansoprazole (Rs = 2.91) and rabeprazole (Rs = 2.53) enantiomers with favorable migration order (in both cases the S-enantiomers migrates first).
31281995	7	51	dep	buffer	1081:1086	arg1	detection					1192:1200	detection	1192:1200	detection at 210 nm for lansoprazole	1192:1227	The optimized methods (25 mM phosphate buffer, pH 7, 10 mM sulfobutyl-ether-β-CD/20 mM γ-CD, +20 kV voltage; 17°C temperature; 50 mbar/3 s injection, detection at 210 nm for lansoprazole; 25 mM phosphate buffer, pH 7, 15 mM sulfobutyl-ether-β-CD/30 mM γ-CD, +20 kV voltage; 18°C temperature; 50 mbar/3 s injection, detection at 210 nm for rabeprazole) provided baseline separation for lansoprazole (Rs = 2.91) and rabeprazole (Rs = 2.53) enantiomers with favorable migration order (in both cases the S-enantiomers migrates first).
31281995	7	51	dep	buffer	1081:1086	arg1	pH					1089:1090	pH 7	1089:1092	pH 7	1089:1092	The optimized methods (25 mM phosphate buffer, pH 7, 10 mM sulfobutyl-ether-β-CD/20 mM γ-CD, +20 kV voltage; 17°C temperature; 50 mbar/3 s injection, detection at 210 nm for lansoprazole; 25 mM phosphate buffer, pH 7, 15 mM sulfobutyl-ether-β-CD/30 mM γ-CD, +20 kV voltage; 18°C temperature; 50 mbar/3 s injection, detection at 210 nm for rabeprazole) provided baseline separation for lansoprazole (Rs = 2.91) and rabeprazole (Rs = 2.53) enantiomers with favorable migration order (in both cases the S-enantiomers migrates first).
31281995	3	52	theme	chiral	509:514	arg1	Sulfobutyl-ether-β-CD					398:418	Sulfobutyl-ether-β-CD	398:418	Sulfobutyl-ether-β-CD with a degree of substitution of 6.5 and 10 at neutral pH	398:476	Sulfobutyl-ether-β-CD with a degree of substitution of 6.5 and 10 at neutral pH proved to be the most suitable chiral selector for both compounds.
31281995	3	52	theme	chiral	509:514	arg1	selector					516:523	the most suitable chiral selector	491:523	the most suitable chiral selector for both compounds	491:542	Sulfobutyl-ether-β-CD with a degree of substitution of 6.5 and 10 at neutral pH proved to be the most suitable chiral selector for both compounds.
31281995	7	53	dep	order	1517:1521	arg1	migrates					1556:1563	migrates	1556:1563	migrates first	1556:1569	The optimized methods (25 mM phosphate buffer, pH 7, 10 mM sulfobutyl-ether-β-CD/20 mM γ-CD, +20 kV voltage; 17°C temperature; 50 mbar/3 s injection, detection at 210 nm for lansoprazole; 25 mM phosphate buffer, pH 7, 15 mM sulfobutyl-ether-β-CD/30 mM γ-CD, +20 kV voltage; 18°C temperature; 50 mbar/3 s injection, detection at 210 nm for rabeprazole) provided baseline separation for lansoprazole (Rs = 2.91) and rabeprazole (Rs = 2.53) enantiomers with favorable migration order (in both cases the S-enantiomers migrates first).
31281995	7	54	theme	17°C	1151:1154	arg1	temperature					1156:1166	17°C temperature	1151:1166	17°C temperature	1151:1166	The optimized methods (25 mM phosphate buffer, pH 7, 10 mM sulfobutyl-ether-β-CD/20 mM γ-CD, +20 kV voltage; 17°C temperature; 50 mbar/3 s injection, detection at 210 nm for lansoprazole; 25 mM phosphate buffer, pH 7, 15 mM sulfobutyl-ether-β-CD/30 mM γ-CD, +20 kV voltage; 18°C temperature; 50 mbar/3 s injection, detection at 210 nm for rabeprazole) provided baseline separation for lansoprazole (Rs = 2.91) and rabeprazole (Rs = 2.53) enantiomers with favorable migration order (in both cases the S-enantiomers migrates first).
31281995	4	55	theme	possible	597:604	arg1	mechanisms					606:615	the possible mechanisms	593:615	the possible mechanisms of enantiomer separation	593:640	Various dual CD systems were also compared, and the possible mechanisms of enantiomer separation were investigated.
31281995	7	56	dep	pH	1089:1090	arg1	temperature					1156:1166	17°C temperature	1151:1166	17°C temperature	1151:1166	The optimized methods (25 mM phosphate buffer, pH 7, 10 mM sulfobutyl-ether-β-CD/20 mM γ-CD, +20 kV voltage; 17°C temperature; 50 mbar/3 s injection, detection at 210 nm for lansoprazole; 25 mM phosphate buffer, pH 7, 15 mM sulfobutyl-ether-β-CD/30 mM γ-CD, +20 kV voltage; 18°C temperature; 50 mbar/3 s injection, detection at 210 nm for rabeprazole) provided baseline separation for lansoprazole (Rs = 2.91) and rabeprazole (Rs = 2.53) enantiomers with favorable migration order (in both cases the S-enantiomers migrates first).
31281995	7	57	theme	rabeprazole	1456:1466	arg1	enantiomers					1480:1490	rabeprazole (Rs = 2.53) enantiomers	1456:1490	rabeprazole (Rs = 2.53) enantiomers with favorable migration order (in both cases the S-enantiomers migrates first)	1456:1570	The optimized methods (25 mM phosphate buffer, pH 7, 10 mM sulfobutyl-ether-β-CD/20 mM γ-CD, +20 kV voltage; 17°C temperature; 50 mbar/3 s injection, detection at 210 nm for lansoprazole; 25 mM phosphate buffer, pH 7, 15 mM sulfobutyl-ether-β-CD/30 mM γ-CD, +20 kV voltage; 18°C temperature; 50 mbar/3 s injection, detection at 210 nm for rabeprazole) provided baseline separation for lansoprazole (Rs = 2.91) and rabeprazole (Rs = 2.53) enantiomers with favorable migration order (in both cases the S-enantiomers migrates first).
31281995	7	57	theme	rabeprazole	1456:1466	arg1	Rs					1469:1470	Rs = 2.53	1469:1477	Rs = 2.53	1469:1477	The optimized methods (25 mM phosphate buffer, pH 7, 10 mM sulfobutyl-ether-β-CD/20 mM γ-CD, +20 kV voltage; 17°C temperature; 50 mbar/3 s injection, detection at 210 nm for lansoprazole; 25 mM phosphate buffer, pH 7, 15 mM sulfobutyl-ether-β-CD/30 mM γ-CD, +20 kV voltage; 18°C temperature; 50 mbar/3 s injection, detection at 210 nm for rabeprazole) provided baseline separation for lansoprazole (Rs = 2.91) and rabeprazole (Rs = 2.53) enantiomers with favorable migration order (in both cases the S-enantiomers migrates first).
31281995	6	58	theme	Method	819:824	arg1	optimization					826:837	Method optimization	819:837	Method optimization	819:837	Method optimization was carried out using an experimental design approach, performing an initial fractional factorial screening design, followed by a central composite design to establish the optimal analytical conditions.
31281995	2	59	theme	neutral	317:323	arg1	CDs					337:339	Fourteen different neutral and anionic CDs	298:339	Fourteen different neutral and anionic CDs	298:339	Fourteen different neutral and anionic CDs were screened at pH 4 and 7 in the preliminary analysis.
31281995	1	60	theme	pump	281:284	arg1	rabeprazole					257:267	rabeprazole	257:267	rabeprazole	257:267	Novel capillary electrophoresis methods using CDs as chiral selectors were developed and validated for the chiral separation of lansoprazole and rabeprazole, two proton pump inhibitors.
31281995	1	60	theme	pump	281:284	arg1	inhibitors					286:295	two proton pump inhibitors	270:295	two proton pump inhibitors	270:295	Novel capillary electrophoresis methods using CDs as chiral selectors were developed and validated for the chiral separation of lansoprazole and rabeprazole, two proton pump inhibitors.
31281995	6	61	theme	initial	908:914	arg1	design					947:952	an initial fractional factorial screening design	905:952	an initial fractional factorial screening design	905:952	Method optimization was carried out using an experimental design approach, performing an initial fractional factorial screening design, followed by a central composite design to establish the optimal analytical conditions.
31281995	7	62	theme	sulfobutyl-ether-β-CD/20 mM	1101:1127	arg1	γ-CD					1129:1132	10 mM sulfobutyl-ether-β-CD/20 mM γ-CD	1095:1132	10 mM sulfobutyl-ether-β-CD/20 mM γ-CD	1095:1132	The optimized methods (25 mM phosphate buffer, pH 7, 10 mM sulfobutyl-ether-β-CD/20 mM γ-CD, +20 kV voltage; 17°C temperature; 50 mbar/3 s injection, detection at 210 nm for lansoprazole; 25 mM phosphate buffer, pH 7, 15 mM sulfobutyl-ether-β-CD/30 mM γ-CD, +20 kV voltage; 18°C temperature; 50 mbar/3 s injection, detection at 210 nm for rabeprazole) provided baseline separation for lansoprazole (Rs = 2.91) and rabeprazole (Rs = 2.53) enantiomers with favorable migration order (in both cases the S-enantiomers migrates first).
31281995	7	62	theme	sulfobutyl-ether-β-CD/20 mM	1101:1127	arg1	pH					1089:1090	pH 7	1089:1092	pH 7	1089:1092	The optimized methods (25 mM phosphate buffer, pH 7, 10 mM sulfobutyl-ether-β-CD/20 mM γ-CD, +20 kV voltage; 17°C temperature; 50 mbar/3 s injection, detection at 210 nm for lansoprazole; 25 mM phosphate buffer, pH 7, 15 mM sulfobutyl-ether-β-CD/30 mM γ-CD, +20 kV voltage; 18°C temperature; 50 mbar/3 s injection, detection at 210 nm for rabeprazole) provided baseline separation for lansoprazole (Rs = 2.91) and rabeprazole (Rs = 2.53) enantiomers with favorable migration order (in both cases the S-enantiomers migrates first).
31281995	7	63	theme	=	1444:1444	arg1	lansoprazole					1427:1438	lansoprazole	1427:1438	lansoprazole (Rs = 2.91)	1427:1450	The optimized methods (25 mM phosphate buffer, pH 7, 10 mM sulfobutyl-ether-β-CD/20 mM γ-CD, +20 kV voltage; 17°C temperature; 50 mbar/3 s injection, detection at 210 nm for lansoprazole; 25 mM phosphate buffer, pH 7, 15 mM sulfobutyl-ether-β-CD/30 mM γ-CD, +20 kV voltage; 18°C temperature; 50 mbar/3 s injection, detection at 210 nm for rabeprazole) provided baseline separation for lansoprazole (Rs = 2.91) and rabeprazole (Rs = 2.53) enantiomers with favorable migration order (in both cases the S-enantiomers migrates first).
31281995	7	63	theme	=	1444:1444	arg1	Rs					1441:1442	Rs = 2.91	1441:1449	Rs = 2.91	1441:1449	The optimized methods (25 mM phosphate buffer, pH 7, 10 mM sulfobutyl-ether-β-CD/20 mM γ-CD, +20 kV voltage; 17°C temperature; 50 mbar/3 s injection, detection at 210 nm for lansoprazole; 25 mM phosphate buffer, pH 7, 15 mM sulfobutyl-ether-β-CD/30 mM γ-CD, +20 kV voltage; 18°C temperature; 50 mbar/3 s injection, detection at 210 nm for rabeprazole) provided baseline separation for lansoprazole (Rs = 2.91) and rabeprazole (Rs = 2.53) enantiomers with favorable migration order (in both cases the S-enantiomers migrates first).
31281995	2	64	theme	anionic	329:335	arg1	CDs					337:339	Fourteen different neutral and anionic CDs	298:339	Fourteen different neutral and anionic CDs	298:339	Fourteen different neutral and anionic CDs were screened at pH 4 and 7 in the preliminary analysis.
31281995	6	65	theme	composite	977:985	arg1	design					987:992	a central composite design	967:992	a central composite design	967:992	Method optimization was carried out using an experimental design approach, performing an initial fractional factorial screening design, followed by a central composite design to establish the optimal analytical conditions.
31281995	3	66	with	Sulfobutyl-ether-β-CD	398:418	arg1	degree					427:432	a degree	425:432	a degree of substitution of 6.5 and 10 at neutral pH	425:476	Sulfobutyl-ether-β-CD with a degree of substitution of 6.5 and 10 at neutral pH proved to be the most suitable chiral selector for both compounds.
31281995	5	67	theme	substitution	727:738	arg1	degree					717:722	sulfobutyl-ether-β-CD degree	695:722	sulfobutyl-ether-β-CD degree of substitution 6.5 and native γ-CD	695:758	A dual selector system containing sulfobutyl-ether-β-CD degree of substitution 6.5 and native γ-CD proved to be the most adequate system for the separations.
31281995	7	68	from	210 nm	1205:1210	arg1	detection					1192:1200	detection	1192:1200	detection at 210 nm for lansoprazole	1192:1227	The optimized methods (25 mM phosphate buffer, pH 7, 10 mM sulfobutyl-ether-β-CD/20 mM γ-CD, +20 kV voltage; 17°C temperature; 50 mbar/3 s injection, detection at 210 nm for lansoprazole; 25 mM phosphate buffer, pH 7, 15 mM sulfobutyl-ether-β-CD/30 mM γ-CD, +20 kV voltage; 18°C temperature; 50 mbar/3 s injection, detection at 210 nm for rabeprazole) provided baseline separation for lansoprazole (Rs = 2.91) and rabeprazole (Rs = 2.53) enantiomers with favorable migration order (in both cases the S-enantiomers migrates first).
31281995	4	69	theme	separation	631:640	arg1	mechanisms					606:615	the possible mechanisms	593:615	the possible mechanisms of enantiomer separation	593:640	Various dual CD systems were also compared, and the possible mechanisms of enantiomer separation were investigated.
31281995	7	70	theme	25 mM	1065:1069	arg1	buffer					1081:1086	25 mM phosphate buffer	1065:1086	25 mM phosphate buffer	1065:1086	The optimized methods (25 mM phosphate buffer, pH 7, 10 mM sulfobutyl-ether-β-CD/20 mM γ-CD, +20 kV voltage; 17°C temperature; 50 mbar/3 s injection, detection at 210 nm for lansoprazole; 25 mM phosphate buffer, pH 7, 15 mM sulfobutyl-ether-β-CD/30 mM γ-CD, +20 kV voltage; 18°C temperature; 50 mbar/3 s injection, detection at 210 nm for rabeprazole) provided baseline separation for lansoprazole (Rs = 2.91) and rabeprazole (Rs = 2.53) enantiomers with favorable migration order (in both cases the S-enantiomers migrates first).
31281995	7	71	theme	optimized	1046:1054	arg1	methods					1056:1062	The optimized methods	1042:1062	The optimized methods	1042:1062	The optimized methods (25 mM phosphate buffer, pH 7, 10 mM sulfobutyl-ether-β-CD/20 mM γ-CD, +20 kV voltage; 17°C temperature; 50 mbar/3 s injection, detection at 210 nm for lansoprazole; 25 mM phosphate buffer, pH 7, 15 mM sulfobutyl-ether-β-CD/30 mM γ-CD, +20 kV voltage; 18°C temperature; 50 mbar/3 s injection, detection at 210 nm for rabeprazole) provided baseline separation for lansoprazole (Rs = 2.91) and rabeprazole (Rs = 2.53) enantiomers with favorable migration order (in both cases the S-enantiomers migrates first).
31281995	1	72	theme	electrophoresis	128:142	arg1	methods					144:150	Novel capillary electrophoresis methods	112:150	Novel capillary electrophoresis methods using CDs as chiral selectors	112:180	Novel capillary electrophoresis methods using CDs as chiral selectors were developed and validated for the chiral separation of lansoprazole and rabeprazole, two proton pump inhibitors.
31281995	8	73	theme	0.15	1724:1727	arg1	%					1728:1728	%	1728:1728	%	1728:1728	The optimized methods were validated according to current guidelines and proved to be reliable, linear, precise, and accurate for the determination of 0.15% distomer as chiral impurity in dexlansoprazole and dexrabeprazole samples.
31281995	7	74	theme	25 mM	1230:1234	arg1	pH					1254:1255	pH 7	1254:1257	pH 7	1254:1257	The optimized methods (25 mM phosphate buffer, pH 7, 10 mM sulfobutyl-ether-β-CD/20 mM γ-CD, +20 kV voltage; 17°C temperature; 50 mbar/3 s injection, detection at 210 nm for lansoprazole; 25 mM phosphate buffer, pH 7, 15 mM sulfobutyl-ether-β-CD/30 mM γ-CD, +20 kV voltage; 18°C temperature; 50 mbar/3 s injection, detection at 210 nm for rabeprazole) provided baseline separation for lansoprazole (Rs = 2.91) and rabeprazole (Rs = 2.53) enantiomers with favorable migration order (in both cases the S-enantiomers migrates first).
31281995	7	74	theme	25 mM	1230:1234	arg1	buffer					1246:1251	25 mM phosphate buffer	1230:1251	25 mM phosphate buffer	1230:1251	The optimized methods (25 mM phosphate buffer, pH 7, 10 mM sulfobutyl-ether-β-CD/20 mM γ-CD, +20 kV voltage; 17°C temperature; 50 mbar/3 s injection, detection at 210 nm for lansoprazole; 25 mM phosphate buffer, pH 7, 15 mM sulfobutyl-ether-β-CD/30 mM γ-CD, +20 kV voltage; 18°C temperature; 50 mbar/3 s injection, detection at 210 nm for rabeprazole) provided baseline separation for lansoprazole (Rs = 2.91) and rabeprazole (Rs = 2.53) enantiomers with favorable migration order (in both cases the S-enantiomers migrates first).
31281995	8	75	theme	distomer	1730:1737	arg1	determination					1707:1719	the determination	1703:1719	the determination of 0.15% distomer	1703:1737	The optimized methods were validated according to current guidelines and proved to be reliable, linear, precise, and accurate for the determination of 0.15% distomer as chiral impurity in dexlansoprazole and dexrabeprazole samples.
31281995	0	76	theme	cyclodextrin	90:101	arg1	systems					103:109	dual cyclodextrin systems	85:109	dual cyclodextrin systems	85:109	Chiral separation of lansoprazole and rabeprazole by capillary electrophoresis using dual cyclodextrin systems.
31281995	8	77	theme	chiral	1742:1747	arg1	impurity					1749:1756	chiral impurity	1742:1756	chiral impurity in dexlansoprazole and dexrabeprazole samples	1742:1802	The optimized methods were validated according to current guidelines and proved to be reliable, linear, precise, and accurate for the determination of 0.15% distomer as chiral impurity in dexlansoprazole and dexrabeprazole samples.
31281995	6	78	theme	analytical	1019:1028	arg1	conditions					1030:1039	the optimal analytical conditions	1007:1039	the optimal analytical conditions	1007:1039	Method optimization was carried out using an experimental design approach, performing an initial fractional factorial screening design, followed by a central composite design to establish the optimal analytical conditions.
31281995	5	79	theme	γ-CD	755:758	arg1	degree					717:722	sulfobutyl-ether-β-CD degree	695:722	sulfobutyl-ether-β-CD degree of substitution 6.5 and native γ-CD	695:758	A dual selector system containing sulfobutyl-ether-β-CD degree of substitution 6.5 and native γ-CD proved to be the most adequate system for the separations.
31281995	7	80	theme	18°C	1316:1319	arg1	temperature					1321:1331	18°C temperature	1316:1331	18°C temperature	1316:1331	The optimized methods (25 mM phosphate buffer, pH 7, 10 mM sulfobutyl-ether-β-CD/20 mM γ-CD, +20 kV voltage; 17°C temperature; 50 mbar/3 s injection, detection at 210 nm for lansoprazole; 25 mM phosphate buffer, pH 7, 15 mM sulfobutyl-ether-β-CD/30 mM γ-CD, +20 kV voltage; 18°C temperature; 50 mbar/3 s injection, detection at 210 nm for rabeprazole) provided baseline separation for lansoprazole (Rs = 2.91) and rabeprazole (Rs = 2.53) enantiomers with favorable migration order (in both cases the S-enantiomers migrates first).
31281995	4	81	theme	dual	553:556	arg1	systems					561:567	Various dual CD systems	545:567	Various dual CD systems	545:567	Various dual CD systems were also compared, and the possible mechanisms of enantiomer separation were investigated.
31281995	2	82	theme	preliminary	376:386	arg1	analysis					388:395	the preliminary analysis	372:395	the preliminary analysis	372:395	Fourteen different neutral and anionic CDs were screened at pH 4 and 7 in the preliminary analysis.
31281995	0	83	theme	lansoprazole	21:32	arg1	separation					7:16	Chiral separation	0:16	Chiral separation of lansoprazole and rabeprazole by capillary electrophoresis	0:77	Chiral separation of lansoprazole and rabeprazole by capillary electrophoresis using dual cyclodextrin systems.
31281995	7	84	dep	pH	1254:1255	arg1	temperature					1321:1331	18°C temperature	1316:1331	18°C temperature	1316:1331	The optimized methods (25 mM phosphate buffer, pH 7, 10 mM sulfobutyl-ether-β-CD/20 mM γ-CD, +20 kV voltage; 17°C temperature; 50 mbar/3 s injection, detection at 210 nm for lansoprazole; 25 mM phosphate buffer, pH 7, 15 mM sulfobutyl-ether-β-CD/30 mM γ-CD, +20 kV voltage; 18°C temperature; 50 mbar/3 s injection, detection at 210 nm for rabeprazole) provided baseline separation for lansoprazole (Rs = 2.91) and rabeprazole (Rs = 2.53) enantiomers with favorable migration order (in both cases the S-enantiomers migrates first).
31281995	7	84	dep	pH	1254:1255	arg1	injection					1346:1354	50 mbar/3 s injection	1334:1354	50 mbar/3 s injection	1334:1354	The optimized methods (25 mM phosphate buffer, pH 7, 10 mM sulfobutyl-ether-β-CD/20 mM γ-CD, +20 kV voltage; 17°C temperature; 50 mbar/3 s injection, detection at 210 nm for lansoprazole; 25 mM phosphate buffer, pH 7, 15 mM sulfobutyl-ether-β-CD/30 mM γ-CD, +20 kV voltage; 18°C temperature; 50 mbar/3 s injection, detection at 210 nm for rabeprazole) provided baseline separation for lansoprazole (Rs = 2.91) and rabeprazole (Rs = 2.53) enantiomers with favorable migration order (in both cases the S-enantiomers migrates first).
31281995	7	85	from	210 nm	1370:1375	arg1	pH					1254:1255	pH 7	1254:1257	pH 7	1254:1257	The optimized methods (25 mM phosphate buffer, pH 7, 10 mM sulfobutyl-ether-β-CD/20 mM γ-CD, +20 kV voltage; 17°C temperature; 50 mbar/3 s injection, detection at 210 nm for lansoprazole; 25 mM phosphate buffer, pH 7, 15 mM sulfobutyl-ether-β-CD/30 mM γ-CD, +20 kV voltage; 18°C temperature; 50 mbar/3 s injection, detection at 210 nm for rabeprazole) provided baseline separation for lansoprazole (Rs = 2.91) and rabeprazole (Rs = 2.53) enantiomers with favorable migration order (in both cases the S-enantiomers migrates first).
31281995	7	85	from	210 nm	1370:1375	arg1	buffer					1246:1251	25 mM phosphate buffer	1230:1251	25 mM phosphate buffer	1230:1251	The optimized methods (25 mM phosphate buffer, pH 7, 10 mM sulfobutyl-ether-β-CD/20 mM γ-CD, +20 kV voltage; 17°C temperature; 50 mbar/3 s injection, detection at 210 nm for lansoprazole; 25 mM phosphate buffer, pH 7, 15 mM sulfobutyl-ether-β-CD/30 mM γ-CD, +20 kV voltage; 18°C temperature; 50 mbar/3 s injection, detection at 210 nm for rabeprazole) provided baseline separation for lansoprazole (Rs = 2.91) and rabeprazole (Rs = 2.53) enantiomers with favorable migration order (in both cases the S-enantiomers migrates first).
31281995	5	86	theme	adequate	782:789	arg1	system					791:796	the most adequate system	773:796	the most adequate system for the separations	773:816	A dual selector system containing sulfobutyl-ether-β-CD degree of substitution 6.5 and native γ-CD proved to be the most adequate system for the separations.
31281995	5	86	theme	adequate	782:789	arg1	system					677:682	A dual selector system	661:682	A dual selector system containing sulfobutyl-ether-β-CD degree of substitution 6.5 and native γ-CD	661:758	A dual selector system containing sulfobutyl-ether-β-CD degree of substitution 6.5 and native γ-CD proved to be the most adequate system for the separations.
31281995	6	87	theme	design	877:882	arg1	approach					884:891	an experimental design approach	861:891	an experimental design approach	861:891	Method optimization was carried out using an experimental design approach, performing an initial fractional factorial screening design, followed by a central composite design to establish the optimal analytical conditions.
31281995	1	88	theme	rabeprazole	257:267	arg1	separation					226:235	the chiral separation	215:235	the chiral separation of lansoprazole and rabeprazole, two proton pump inhibitors	215:295	Novel capillary electrophoresis methods using CDs as chiral selectors were developed and validated for the chiral separation of lansoprazole and rabeprazole, two proton pump inhibitors.
31281995	0	89	theme	capillary	53:61	arg1	electrophoresis					63:77	capillary electrophoresis	53:77	capillary electrophoresis	53:77	Chiral separation of lansoprazole and rabeprazole by capillary electrophoresis using dual cyclodextrin systems.
31281995	7	90	theme	migration	1507:1515	arg1	order					1517:1521	favorable migration order	1497:1521	favorable migration order (in both cases the S-enantiomers migrates first)	1497:1570	The optimized methods (25 mM phosphate buffer, pH 7, 10 mM sulfobutyl-ether-β-CD/20 mM γ-CD, +20 kV voltage; 17°C temperature; 50 mbar/3 s injection, detection at 210 nm for lansoprazole; 25 mM phosphate buffer, pH 7, 15 mM sulfobutyl-ether-β-CD/30 mM γ-CD, +20 kV voltage; 18°C temperature; 50 mbar/3 s injection, detection at 210 nm for rabeprazole) provided baseline separation for lansoprazole (Rs = 2.91) and rabeprazole (Rs = 2.53) enantiomers with favorable migration order (in both cases the S-enantiomers migrates first).
31281995	7	91	theme	sulfobutyl-ether-β-CD/30 mM	1266:1292	arg1	pH					1254:1255	pH 7	1254:1257	pH 7	1254:1257	The optimized methods (25 mM phosphate buffer, pH 7, 10 mM sulfobutyl-ether-β-CD/20 mM γ-CD, +20 kV voltage; 17°C temperature; 50 mbar/3 s injection, detection at 210 nm for lansoprazole; 25 mM phosphate buffer, pH 7, 15 mM sulfobutyl-ether-β-CD/30 mM γ-CD, +20 kV voltage; 18°C temperature; 50 mbar/3 s injection, detection at 210 nm for rabeprazole) provided baseline separation for lansoprazole (Rs = 2.91) and rabeprazole (Rs = 2.53) enantiomers with favorable migration order (in both cases the S-enantiomers migrates first).
31281995	7	91	theme	sulfobutyl-ether-β-CD/30 mM	1266:1292	arg1	γ-CD					1294:1297	15 mM sulfobutyl-ether-β-CD/30 mM γ-CD	1260:1297	15 mM sulfobutyl-ether-β-CD/30 mM γ-CD	1260:1297	The optimized methods (25 mM phosphate buffer, pH 7, 10 mM sulfobutyl-ether-β-CD/20 mM γ-CD, +20 kV voltage; 17°C temperature; 50 mbar/3 s injection, detection at 210 nm for lansoprazole; 25 mM phosphate buffer, pH 7, 15 mM sulfobutyl-ether-β-CD/30 mM γ-CD, +20 kV voltage; 18°C temperature; 50 mbar/3 s injection, detection at 210 nm for rabeprazole) provided baseline separation for lansoprazole (Rs = 2.91) and rabeprazole (Rs = 2.53) enantiomers with favorable migration order (in both cases the S-enantiomers migrates first).
31281995	1	92	theme	chiral	165:170	arg1	CDs					158:160	CDs	158:160	CDs	158:160	Novel capillary electrophoresis methods using CDs as chiral selectors were developed and validated for the chiral separation of lansoprazole and rabeprazole, two proton pump inhibitors.
31281995	1	92	theme	chiral	165:170	arg1	selectors					172:180	chiral selectors	165:180	chiral selectors	165:180	Novel capillary electrophoresis methods using CDs as chiral selectors were developed and validated for the chiral separation of lansoprazole and rabeprazole, two proton pump inhibitors.
31281995	8	93	theme	optimized	1577:1585	arg1	methods					1587:1593	The optimized methods	1573:1593	The optimized methods	1573:1593	The optimized methods were validated according to current guidelines and proved to be reliable, linear, precise, and accurate for the determination of 0.15% distomer as chiral impurity in dexlansoprazole and dexrabeprazole samples.
30447364	0	0	theme	bone	80:83	arg1	engineering					92:102	bone tissue engineering	80:102	bone tissue engineering	80:102	Sequential releasing of VEGF and BMP-2 in hydroxyapatite collagen scaffolds for bone tissue engineering: Design and characterization.
30447364	8	1	theme	in	1038:1039	arg1	experiments					1059:1069	in vitro and in vivo experiments	1038:1069	in vitro and in vivo experiments	1038:1069	The biocompatibility of the sustained-release system was evaluated by in vitro and in vivo experiments.
30447364	1	2	theme	biomaterials	150:161	arg1	property					138:145	The property	134:145	The property of biomaterials to control the sequential release of growth factors	134:213	The property of biomaterials to control the sequential release of growth factors has been widely concerned in the field of bone repair and regeneration.
30447364	9	3	dep	in	1270:1271	arg1	vitro					1273:1277	vitro	1273:1277	vitro	1273:1277	HC composite scaffolds were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction detection (XRD), in vitro sustained release test, in vitro cell culture and in vivo animal experiments.
30447364	9	4	theme	in	1237:1238	arg1	test					1264:1267	in vitro sustained release test	1237:1267	in vitro sustained release test	1237:1267	HC composite scaffolds were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction detection (XRD), in vitro sustained release test, in vitro cell culture and in vivo animal experiments.
30447364	10	5	theme	bone	1482:1485	arg1	regeneration					1487:1498	bone regeneration	1482:1498	bone regeneration	1482:1498	The results showed that rhBMP-2/VEGF in HC composite scaffolds (HCBV) successfully achieved the sequential release of the double factors, which could benefit bone regeneration.
30447364	8	6	theme	in	1051:1052	arg1	experiments					1059:1069	in vitro and in vivo experiments	1038:1069	in vitro and in vivo experiments	1038:1069	The biocompatibility of the sustained-release system was evaluated by in vitro and in vivo experiments.
30447364	7	7	theme	composite	922:930	arg1	scaffolds					957:965	double factors composite sustained-release system scaffolds	907:965	double factors composite sustained-release system scaffolds	907:965	These were double factors composite sustained-release system scaffolds.
30447364	3	8	theme	chitosan	389:396	arg1	O-CMCS					412:417	O-CMCS	412:417	O-CMCS	412:417	O‑Carboxymethyl chitosan microspheres (O-CMCS) has antimicrobial activity, biodegradability, biocompatibility and film formation.
30447364	3	8	theme	chitosan	389:396	arg1	microspheres					398:409	O‑Carboxymethyl chitosan microspheres	373:409	O‑Carboxymethyl chitosan microspheres (O-CMCS)	373:418	O‑Carboxymethyl chitosan microspheres (O-CMCS) has antimicrobial activity, biodegradability, biocompatibility and film formation.
30447364	0	9	from	Sequential	0:9	arg1	scaffolds					66:74	hydroxyapatite collagen scaffolds	42:74	hydroxyapatite collagen scaffolds for bone tissue engineering	42:102	Sequential releasing of VEGF and BMP-2 in hydroxyapatite collagen scaffolds for bone tissue engineering: Design and characterization.
30447364	10	10	theme	sequential	1420:1429	arg1	release					1431:1437	the sequential release	1416:1437	the sequential release of the double factors, which could benefit bone regeneration	1416:1498	The results showed that rhBMP-2/VEGF in HC composite scaffolds (HCBV) successfully achieved the sequential release of the double factors, which could benefit bone regeneration.
30447364	5	11	theme	drug	655:658	arg1	carrier					660:666	drug carrier	655:666	drug carrier	655:666	O-CMCS were used as drug carrier to construct the compound sustained-release system with rhBMP-2 and VEGF double factors release.
30447364	5	11	theme	drug	655:658	arg1	O-CMCS					635:640	O-CMCS	635:640	O-CMCS	635:640	O-CMCS were used as drug carrier to construct the compound sustained-release system with rhBMP-2 and VEGF double factors release.
30447364	7	12	theme	sustained-release	932:948	arg1	scaffolds					957:965	double factors composite sustained-release system scaffolds	907:965	double factors composite sustained-release system scaffolds	907:965	These were double factors composite sustained-release system scaffolds.
30447364	3	13	theme	antimicrobial	424:436	arg1	activity					438:445	antimicrobial activity	424:445	antimicrobial activity	424:445	O‑Carboxymethyl chitosan microspheres (O-CMCS) has antimicrobial activity, biodegradability, biocompatibility and film formation.
30447364	0	14	theme	tissue	85:90	arg1	engineering					92:102	bone tissue engineering	80:102	bone tissue engineering	80:102	Sequential releasing of VEGF and BMP-2 in hydroxyapatite collagen scaffolds for bone tissue engineering: Design and characterization.
30447364	4	15	theme	scaffolds	524:532	arg1	materials					534:542	scaffolds materials	524:542	scaffolds materials with microspheres with a purpose of forming a controlled slow release secondary structure	524:632	This study developed scaffolds materials with microspheres with a purpose of forming a controlled slow release secondary structure.
30447364	5	16	with	system	712:717	arg1	release					756:762	VEGF double factors release	736:762	VEGF double factors release	736:762	O-CMCS were used as drug carrier to construct the compound sustained-release system with rhBMP-2 and VEGF double factors release.
30447364	5	16	with	system	712:717	arg1	rhBMP-2					724:730	rhBMP-2	724:730	rhBMP-2	724:730	O-CMCS were used as drug carrier to construct the compound sustained-release system with rhBMP-2 and VEGF double factors release.
30447364	5	17	used	used	647:650	arg2	carrier					660:666	drug carrier	655:666	drug carrier	655:666	O-CMCS were used as drug carrier to construct the compound sustained-release system with rhBMP-2 and VEGF double factors release.
30447364	5	17	used	used	647:650	arg2	O-CMCS					635:640	O-CMCS	635:640	O-CMCS	635:640	O-CMCS were used as drug carrier to construct the compound sustained-release system with rhBMP-2 and VEGF double factors release.
30447364	1	18	theme	bone	257:260	arg1	repair					262:267	bone repair	257:267	bone repair	257:267	The property of biomaterials to control the sequential release of growth factors has been widely concerned in the field of bone repair and regeneration.
30447364	7	19	theme	double	907:912	arg1	scaffolds					957:965	double factors composite sustained-release system scaffolds	907:965	double factors composite sustained-release system scaffolds	907:965	These were double factors composite sustained-release system scaffolds.
30447364	9	20	theme	sustained	1246:1254	arg1	test					1264:1267	in vitro sustained release test	1237:1267	in vitro sustained release test	1237:1267	HC composite scaffolds were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction detection (XRD), in vitro sustained release test, in vitro cell culture and in vivo animal experiments.
30447364	7	21	theme	factors	914:920	arg1	scaffolds					957:965	double factors composite sustained-release system scaffolds	907:965	double factors composite sustained-release system scaffolds	907:965	These were double factors composite sustained-release system scaffolds.
30447364	3	22	theme	O‑Carboxymethyl	373:387	arg1	O-CMCS					412:417	O-CMCS	412:417	O-CMCS	412:417	O‑Carboxymethyl chitosan microspheres (O-CMCS) has antimicrobial activity, biodegradability, biocompatibility and film formation.
30447364	3	22	theme	O‑Carboxymethyl	373:387	arg1	microspheres					398:409	O‑Carboxymethyl chitosan microspheres	373:409	O‑Carboxymethyl chitosan microspheres (O-CMCS)	373:418	O‑Carboxymethyl chitosan microspheres (O-CMCS) has antimicrobial activity, biodegradability, biocompatibility and film formation.
30447364	9	23	theme	release	1256:1262	arg1	test					1264:1267	in vitro sustained release test	1237:1267	in vitro sustained release test	1237:1267	HC composite scaffolds were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction detection (XRD), in vitro sustained release test, in vitro cell culture and in vivo animal experiments.
30447364	3	24	theme	film	487:490	arg1	formation					492:500	film formation	487:500	film formation	487:500	O‑Carboxymethyl chitosan microspheres (O-CMCS) has antimicrobial activity, biodegradability, biocompatibility and film formation.
30447364	5	25	theme	compound	685:692	arg1	system					712:717	the compound sustained-release system	681:717	the compound sustained-release system with rhBMP-2 and VEGF double factors release	681:762	O-CMCS were used as drug carrier to construct the compound sustained-release system with rhBMP-2 and VEGF double factors release.
30447364	4	26	theme	release	606:612	arg1	structure					624:632	a controlled slow release secondary structure	588:632	a controlled slow release secondary structure	588:632	This study developed scaffolds materials with microspheres with a purpose of forming a controlled slow release secondary structure.
30447364	1	27	theme	sequential	178:187	arg1	release					189:195	the sequential release	174:195	the sequential release of growth factors	174:213	The property of biomaterials to control the sequential release of growth factors has been widely concerned in the field of bone repair and regeneration.
30447364	1	28	theme	repair	262:267	arg1	field					248:252	the field	244:252	the field of bone repair and regeneration	244:284	The property of biomaterials to control the sequential release of growth factors has been widely concerned in the field of bone repair and regeneration.
30447364	5	29	theme	sustained-release	694:710	arg1	system					712:717	the compound sustained-release system	681:717	the compound sustained-release system with rhBMP-2 and VEGF double factors release	681:762	O-CMCS were used as drug carrier to construct the compound sustained-release system with rhBMP-2 and VEGF double factors release.
30447364	3	30	contain	has	420:422	arg2	biodegradability					448:463	biodegradability	448:463	biodegradability	448:463	O‑Carboxymethyl chitosan microspheres (O-CMCS) has antimicrobial activity, biodegradability, biocompatibility and film formation.
30447364	3	30	contain	has	420:422	arg2	formation					492:500	film formation	487:500	film formation	487:500	O‑Carboxymethyl chitosan microspheres (O-CMCS) has antimicrobial activity, biodegradability, biocompatibility and film formation.
30447364	3	30	contain	has	420:422	arg1	microspheres					398:409	O‑Carboxymethyl chitosan microspheres	373:409	O‑Carboxymethyl chitosan microspheres (O-CMCS)	373:418	O‑Carboxymethyl chitosan microspheres (O-CMCS) has antimicrobial activity, biodegradability, biocompatibility and film formation.
30447364	3	30	contain	has	420:422	arg2	biocompatibility					466:481	biocompatibility	466:481	biocompatibility	466:481	O‑Carboxymethyl chitosan microspheres (O-CMCS) has antimicrobial activity, biodegradability, biocompatibility and film formation.
30447364	3	30	contain	has	420:422	arg2	activity					438:445	antimicrobial activity	424:445	antimicrobial activity	424:445	O‑Carboxymethyl chitosan microspheres (O-CMCS) has antimicrobial activity, biodegradability, biocompatibility and film formation.
30447364	3	30	contain	has	420:422	arg1	O-CMCS					412:417	O-CMCS	412:417	O-CMCS	412:417	O‑Carboxymethyl chitosan microspheres (O-CMCS) has antimicrobial activity, biodegradability, biocompatibility and film formation.
30447364	9	31	theme	infrared	1171:1178	arg1	spectroscopy					1180:1191	infrared spectroscopy	1171:1191	infrared spectroscopy	1171:1191	HC composite scaffolds were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction detection (XRD), in vitro sustained release test, in vitro cell culture and in vivo animal experiments.
30447364	0	32	theme	VEGF	24:27	arg1	Sequential					0:9	Sequential	0:9	Sequential	0:9	Sequential releasing of VEGF and BMP-2 in hydroxyapatite collagen scaffolds for bone tissue engineering: Design and characterization.
30447364	4	33	theme	slow	601:604	arg1	structure					624:632	a controlled slow release secondary structure	588:632	a controlled slow release secondary structure	588:632	This study developed scaffolds materials with microspheres with a purpose of forming a controlled slow release secondary structure.
30447364	9	34	theme	in	1270:1271	arg1	culture					1284:1290	in vitro cell culture	1270:1290	in vitro cell culture	1270:1290	HC composite scaffolds were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction detection (XRD), in vitro sustained release test, in vitro cell culture and in vivo animal experiments.
30447364	2	35	theme	sustained-release	301:317	arg1	scaffolds					319:327	Double-factor sustained-release scaffolds	287:327	Double-factor sustained-release scaffolds	287:327	Double-factor sustained-release scaffolds have good biocompatibility and bioactivity.
30447364	10	36	theme	double	1446:1451	arg1	factors					1453:1459	the double factors	1442:1459	the double factors	1442:1459	The results showed that rhBMP-2/VEGF in HC composite scaffolds (HCBV) successfully achieved the sequential release of the double factors, which could benefit bone regeneration.
30447364	10	37	theme	composite	1367:1375	arg1	HCBV					1388:1391	HCBV	1388:1391	HCBV	1388:1391	The results showed that rhBMP-2/VEGF in HC composite scaffolds (HCBV) successfully achieved the sequential release of the double factors, which could benefit bone regeneration.
30447364	10	37	theme	composite	1367:1375	arg1	scaffolds					1377:1385	HC composite scaffolds	1364:1385	HC composite scaffolds (HCBV)	1364:1392	The results showed that rhBMP-2/VEGF in HC composite scaffolds (HCBV) successfully achieved the sequential release of the double factors, which could benefit bone regeneration.
30447364	1	38	theme	regeneration	273:284	arg1	field					248:252	the field	244:252	the field of bone repair and regeneration	244:284	The property of biomaterials to control the sequential release of growth factors has been widely concerned in the field of bone repair and regeneration.
30447364	9	39	theme	X-ray	1202:1206	arg1	diffraction					1208:1218	X-ray diffraction	1202:1218	X-ray diffraction detection (XRD)	1202:1234	HC composite scaffolds were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction detection (XRD), in vitro sustained release test, in vitro cell culture and in vivo animal experiments.
30447364	2	40	theme	Double-factor	287:299	arg1	scaffolds					319:327	Double-factor sustained-release scaffolds	287:327	Double-factor sustained-release scaffolds	287:327	Double-factor sustained-release scaffolds have good biocompatibility and bioactivity.
30447364	8	41	dep	in	1051:1052	arg1	vivo					1054:1057	vivo	1054:1057	vivo	1054:1057	The biocompatibility of the sustained-release system was evaluated by in vitro and in vivo experiments.
30447364	1	42	theme	growth	200:205	arg1	factors					207:213	growth factors	200:213	growth factors	200:213	The property of biomaterials to control the sequential release of growth factors has been widely concerned in the field of bone repair and regeneration.
30447364	0	43	theme	BMP-2	33:37	arg1	Sequential					0:9	Sequential	0:9	Sequential	0:9	Sequential releasing of VEGF and BMP-2 in hydroxyapatite collagen scaffolds for bone tissue engineering: Design and characterization.
30447364	4	44	theme	secondary	614:622	arg1	structure					624:632	a controlled slow release secondary structure	588:632	a controlled slow release secondary structure	588:632	This study developed scaffolds materials with microspheres with a purpose of forming a controlled slow release secondary structure.
30447364	9	45	theme	cell	1279:1282	arg1	culture					1284:1290	in vitro cell culture	1270:1290	in vitro cell culture	1270:1290	HC composite scaffolds were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction detection (XRD), in vitro sustained release test, in vitro cell culture and in vivo animal experiments.
30447364	9	46	dep	in	1237:1238	arg1	vitro					1240:1244	vitro	1240:1244	vitro	1240:1244	HC composite scaffolds were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction detection (XRD), in vitro sustained release test, in vitro cell culture and in vivo animal experiments.
30447364	1	47	theme	factors	207:213	arg1	release					189:195	the sequential release	174:195	the sequential release of growth factors	174:213	The property of biomaterials to control the sequential release of growth factors has been widely concerned in the field of bone repair and regeneration.
30447364	9	48	theme	HC	1072:1073	arg1	scaffolds					1085:1093	HC composite scaffolds	1072:1093	HC composite scaffolds	1072:1093	HC composite scaffolds were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction detection (XRD), in vitro sustained release test, in vitro cell culture and in vivo animal experiments.
30447364	0	49	from	scaffolds	66:74	arg1	Sequential					0:9	Sequential	0:9	Sequential	0:9	Sequential releasing of VEGF and BMP-2 in hydroxyapatite collagen scaffolds for bone tissue engineering: Design and characterization.
30447364	8	50	theme	sustained-release	996:1012	arg1	system					1014:1019	the sustained-release system	992:1019	the sustained-release system	992:1019	The biocompatibility of the sustained-release system was evaluated by in vitro and in vivo experiments.
30447364	0	51	theme	hydroxyapatite	42:55	arg1	scaffolds					66:74	hydroxyapatite collagen scaffolds	42:74	hydroxyapatite collagen scaffolds for bone tissue engineering	42:102	Sequential releasing of VEGF and BMP-2 in hydroxyapatite collagen scaffolds for bone tissue engineering: Design and characterization.
30447364	5	52	theme	VEGF	736:739	arg1	release					756:762	VEGF double factors release	736:762	VEGF double factors release	736:762	O-CMCS were used as drug carrier to construct the compound sustained-release system with rhBMP-2 and VEGF double factors release.
30447364	6	53	theme	collagen	824:831	arg1	scaffolds					838:846	the hydroxyapatite collagen (HC) scaffolds	805:846	the hydroxyapatite collagen (HC) scaffolds	805:846	The O-CMCS were loaded with rhBMP-2 and the hydroxyapatite collagen (HC) scaffolds were loaded with VEGF to prepare the scaffolds.
30447364	4	54	theme	controlled	590:599	arg1	structure					624:632	a controlled slow release secondary structure	588:632	a controlled slow release secondary structure	588:632	This study developed scaffolds materials with microspheres with a purpose of forming a controlled slow release secondary structure.
30447364	5	55	theme	double	741:746	arg1	release					756:762	VEGF double factors release	736:762	VEGF double factors release	736:762	O-CMCS were used as drug carrier to construct the compound sustained-release system with rhBMP-2 and VEGF double factors release.
30447364	9	56	theme	in	1296:1297	arg1	experiments					1311:1321	in vivo animal experiments	1296:1321	in vivo animal experiments	1296:1321	HC composite scaffolds were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction detection (XRD), in vitro sustained release test, in vitro cell culture and in vivo animal experiments.
30447364	10	57	theme	factors	1453:1459	arg1	release					1431:1437	the sequential release	1416:1437	the sequential release of the double factors, which could benefit bone regeneration	1416:1498	The results showed that rhBMP-2/VEGF in HC composite scaffolds (HCBV) successfully achieved the sequential release of the double factors, which could benefit bone regeneration.
30447364	6	58	theme	hydroxyapatite	809:822	arg1	HC					834:835	HC	834:835	HC	834:835	The O-CMCS were loaded with rhBMP-2 and the hydroxyapatite collagen (HC) scaffolds were loaded with VEGF to prepare the scaffolds.
30447364	6	58	theme	hydroxyapatite	809:822	arg1	collagen					824:831	hydroxyapatite collagen	809:831	the hydroxyapatite collagen (HC) scaffolds	805:846	The O-CMCS were loaded with rhBMP-2 and the hydroxyapatite collagen (HC) scaffolds were loaded with VEGF to prepare the scaffolds.
30447364	2	59	contain	have	329:332	arg2	bioactivity					360:370	bioactivity	360:370	bioactivity	360:370	Double-factor sustained-release scaffolds have good biocompatibility and bioactivity.
30447364	2	59	contain	have	329:332	arg1	scaffolds					319:327	Double-factor sustained-release scaffolds	287:327	Double-factor sustained-release scaffolds	287:327	Double-factor sustained-release scaffolds have good biocompatibility and bioactivity.
30447364	2	59	contain	have	329:332	arg2	biocompatibility					339:354	biocompatibility	339:354	biocompatibility	339:354	Double-factor sustained-release scaffolds have good biocompatibility and bioactivity.
30447364	5	60	theme	factors	748:754	arg1	release					756:762	VEGF double factors release	736:762	VEGF double factors release	736:762	O-CMCS were used as drug carrier to construct the compound sustained-release system with rhBMP-2 and VEGF double factors release.
30447364	10	61	from	rhBMP-2/VEGF	1348:1359	arg1	HCBV					1388:1391	HCBV	1388:1391	HCBV	1388:1391	The results showed that rhBMP-2/VEGF in HC composite scaffolds (HCBV) successfully achieved the sequential release of the double factors, which could benefit bone regeneration.
30447364	10	61	from	rhBMP-2/VEGF	1348:1359	arg1	scaffolds					1377:1385	HC composite scaffolds	1364:1385	HC composite scaffolds (HCBV)	1364:1392	The results showed that rhBMP-2/VEGF in HC composite scaffolds (HCBV) successfully achieved the sequential release of the double factors, which could benefit bone regeneration.
30447364	8	62	theme	system	1014:1019	arg1	biocompatibility					972:987	The biocompatibility	968:987	The biocompatibility of the sustained-release system	968:1019	The biocompatibility of the sustained-release system was evaluated by in vitro and in vivo experiments.
30447364	10	63	theme	HC	1364:1365	arg1	HCBV					1388:1391	HCBV	1388:1391	HCBV	1388:1391	The results showed that rhBMP-2/VEGF in HC composite scaffolds (HCBV) successfully achieved the sequential release of the double factors, which could benefit bone regeneration.
30447364	10	63	theme	HC	1364:1365	arg1	scaffolds					1377:1385	HC composite scaffolds	1364:1385	HC composite scaffolds (HCBV)	1364:1392	The results showed that rhBMP-2/VEGF in HC composite scaffolds (HCBV) successfully achieved the sequential release of the double factors, which could benefit bone regeneration.
30447364	8	64	dep	in	1038:1039	arg1	vitro					1041:1045	vitro	1041:1045	vitro	1041:1045	The biocompatibility of the sustained-release system was evaluated by in vitro and in vivo experiments.
30447364	9	65	theme	diffraction	1208:1218	arg1	XRD					1231:1233	XRD	1231:1233	XRD	1231:1233	HC composite scaffolds were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction detection (XRD), in vitro sustained release test, in vitro cell culture and in vivo animal experiments.
30447364	9	65	theme	diffraction	1208:1218	arg1	detection					1220:1228	X-ray diffraction detection	1202:1228	X-ray diffraction detection (XRD)	1202:1234	HC composite scaffolds were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction detection (XRD), in vitro sustained release test, in vitro cell culture and in vivo animal experiments.
30447364	9	66	theme	animal	1304:1309	arg1	experiments					1311:1321	in vivo animal experiments	1296:1321	in vivo animal experiments	1296:1321	HC composite scaffolds were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction detection (XRD), in vitro sustained release test, in vitro cell culture and in vivo animal experiments.
30447364	9	67	dep	Fourier	1153:1159	arg1	transform					1161:1169	transform	1161:1169	transform infrared spectroscopy	1161:1191	HC composite scaffolds were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction detection (XRD), in vitro sustained release test, in vitro cell culture and in vivo animal experiments.
30447364	9	68	dep	in	1296:1297	arg1	vivo					1299:1302	vivo	1299:1302	vivo	1299:1302	HC composite scaffolds were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction detection (XRD), in vitro sustained release test, in vitro cell culture and in vivo animal experiments.
30447364	2	69	theme	good	334:337	arg1	biocompatibility					339:354	biocompatibility	339:354	biocompatibility	339:354	Double-factor sustained-release scaffolds have good biocompatibility and bioactivity.
30447364	9	70	theme	electron	1126:1133	arg1	microscopy					1135:1144	scanning electron microscopy	1117:1144	scanning electron microscopy (SEM)	1117:1150	HC composite scaffolds were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction detection (XRD), in vitro sustained release test, in vitro cell culture and in vivo animal experiments.
30447364	9	70	theme	electron	1126:1133	arg1	SEM					1147:1149	SEM	1147:1149	SEM	1147:1149	HC composite scaffolds were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction detection (XRD), in vitro sustained release test, in vitro cell culture and in vivo animal experiments.
30447364	9	71	theme	scanning	1117:1124	arg1	microscopy					1135:1144	scanning electron microscopy	1117:1144	scanning electron microscopy (SEM)	1117:1150	HC composite scaffolds were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction detection (XRD), in vitro sustained release test, in vitro cell culture and in vivo animal experiments.
30447364	9	71	theme	scanning	1117:1124	arg1	SEM					1147:1149	SEM	1147:1149	SEM	1147:1149	HC composite scaffolds were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction detection (XRD), in vitro sustained release test, in vitro cell culture and in vivo animal experiments.
30447364	9	72	theme	composite	1075:1083	arg1	scaffolds					1085:1093	HC composite scaffolds	1072:1093	HC composite scaffolds	1072:1093	HC composite scaffolds were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction detection (XRD), in vitro sustained release test, in vitro cell culture and in vivo animal experiments.
30447364	0	73	theme	collagen	57:64	arg1	scaffolds					66:74	hydroxyapatite collagen scaffolds	42:74	hydroxyapatite collagen scaffolds for bone tissue engineering	42:102	Sequential releasing of VEGF and BMP-2 in hydroxyapatite collagen scaffolds for bone tissue engineering: Design and characterization.
30447364	0	74	dep	Sequential	0:9	arg1	characterization					116:131	characterization	116:131	characterization	116:131	Sequential releasing of VEGF and BMP-2 in hydroxyapatite collagen scaffolds for bone tissue engineering: Design and characterization.
30447364	0	74	dep	Sequential	0:9	arg1	Design					105:110	Design	105:110	Design	105:110	Sequential releasing of VEGF and BMP-2 in hydroxyapatite collagen scaffolds for bone tissue engineering: Design and characterization.
30447364	4	75	with	materials	534:542	arg1	microspheres					549:560	microspheres	549:560	microspheres	549:560	This study developed scaffolds materials with microspheres with a purpose of forming a controlled slow release secondary structure.
30447364	4	75	with	materials	534:542	arg1	purpose					569:575	a purpose	567:575	a purpose of forming a controlled slow release secondary structure	567:632	This study developed scaffolds materials with microspheres with a purpose of forming a controlled slow release secondary structure.
30447364	7	76	theme	system	950:955	arg1	scaffolds					957:965	double factors composite sustained-release system scaffolds	907:965	double factors composite sustained-release system scaffolds	907:965	These were double factors composite sustained-release system scaffolds.
31297341	3	0	theme	gene	633:636	arg1	sequencing					647:656	rRNA gene amplicon sequencing	628:656	rRNA gene amplicon sequencing	628:656	Methods: Gut microbiota profiles of women with PE and healthy pregnant women in the third trimester and at 1 and 6 weeks postpartum were assessed by 16S rRNA gene amplicon sequencing.
31297341	2	1	theme	early-onset	340:350	arg1	preeclampsia					352:363	early-onset preeclampsia	340:363	early-onset preeclampsia (PE)	340:368	In the present study, we examined the changes of gut microbiota in women with early-onset preeclampsia (PE) and in normotensive, uncomplicated pregnant women during late pregnancy and at 1 and 6 weeks postpartum.
31297341	2	1	theme	early-onset	340:350	arg1	PE					366:367	PE	366:367	PE	366:367	In the present study, we examined the changes of gut microbiota in women with early-onset preeclampsia (PE) and in normotensive, uncomplicated pregnant women during late pregnancy and at 1 and 6 weeks postpartum.
31297341	4	2	theme	third	805:809	arg1	trimesters					811:820	the third trimesters	801:820	the third trimesters	801:820	Plasma levels of interleukin-6 (IL-6), intestinal fatty acid-binding protein (I-FABP), zonulin, and lipopolysaccharide (LPS) were measured in the third trimesters.
31297341	9	3	theme	PE-associated	1818:1830	arg1	alterations					1842:1852	the PE-associated microbial alterations	1814:1852	the PE-associated microbial alterations	1814:1852	In terms of postpartum women, both the gut microbial composition and the PE-associated microbial alterations were highly consistent with those of the antepartum women.
31297341	9	3	theme	PE-associated	1818:1830	arg1	consistent					1866:1875	consistent	1866:1875	consistent	1866:1875	In terms of postpartum women, both the gut microbial composition and the PE-associated microbial alterations were highly consistent with those of the antepartum women.
31297341	9	4	from	terms	1748:1752	arg1	alterations					1842:1852	the PE-associated microbial alterations	1814:1852	the PE-associated microbial alterations	1814:1852	In terms of postpartum women, both the gut microbial composition and the PE-associated microbial alterations were highly consistent with those of the antepartum women.
31297341	9	4	from	terms	1748:1752	arg1	consistent					1866:1875	consistent	1866:1875	consistent	1866:1875	In terms of postpartum women, both the gut microbial composition and the PE-associated microbial alterations were highly consistent with those of the antepartum women.
31297341	9	4	from	terms	1748:1752	arg1	composition					1798:1808	the gut microbial composition	1780:1808	the gut microbial composition	1780:1808	In terms of postpartum women, both the gut microbial composition and the PE-associated microbial alterations were highly consistent with those of the antepartum women.
31297341	11	5	theme	antepartum	2216:2225	arg1	alterations					2227:2237	these antepartum alterations	2210:2237	these antepartum alterations in gut microbiota	2210:2255	Moreover, these antepartum alterations in gut microbiota persisted 6 weeks postpartum.
31297341	3	6	theme	women	511:515	arg1	profiles					499:506	Gut microbiota profiles	484:506	Gut microbiota profiles of women with PE and healthy pregnant women in the third trimester and at 1 and 6 weeks postpartum	484:605	Methods: Gut microbiota profiles of women with PE and healthy pregnant women in the third trimester and at 1 and 6 weeks postpartum were assessed by 16S rRNA gene amplicon sequencing.
31297341	7	7	theme	PE-enriched	1327:1337	arg1	genera					1339:1344	the PE-enriched genera	1323:1344	the PE-enriched genera such as Anaerococcus, Ruminococcus2, Oribacterium, and Bilophila	1323:1409	Maternal blood pressure and liver enzyme levels were positively correlated to the PE-enriched genera such as Anaerococcus, Ruminococcus2, Oribacterium, and Bilophila, while the fetal features (e.g., Apgar score and newborn birth weight) were positively correlated with PE-depleted genera and negatively correlated with PE-enriched genera.
31297341	10	8	theme	uncomplicated	2046:2058	arg1	women					2069:2073	uncomplicated pregnant women	2046:2073	uncomplicated pregnant women	2046:2073	Conclusion: PE diagnosed in the third trimester of pregnancy is associated with a disrupted gut microbiota composition compared with uncomplicated pregnant women, which are associated with maternal clinical features (blood pressure level and liver dysfunction) and newborn birth weight.
31297341	3	9	theme	women	546:550	arg1	profiles					499:506	Gut microbiota profiles	484:506	Gut microbiota profiles of women with PE and healthy pregnant women in the third trimester and at 1 and 6 weeks postpartum	484:605	Methods: Gut microbiota profiles of women with PE and healthy pregnant women in the third trimester and at 1 and 6 weeks postpartum were assessed by 16S rRNA gene amplicon sequencing.
31297341	1	10	theme	metabolic	192:200	arg1	abnormalities					202:214	host metabolic abnormalities	187:214	host metabolic abnormalities	187:214	Background: Imbalances in gut microbiota composition are linked to hypertension, host metabolic abnormalities, systemic inflammation, and other conditions.
31297341	7	11	theme	PE-enriched	1564:1574	arg1	genera					1576:1581	PE-enriched genera	1564:1581	PE-enriched genera	1564:1581	Maternal blood pressure and liver enzyme levels were positively correlated to the PE-enriched genera such as Anaerococcus, Ruminococcus2, Oribacterium, and Bilophila, while the fetal features (e.g., Apgar score and newborn birth weight) were positively correlated with PE-depleted genera and negatively correlated with PE-enriched genera.
31297341	3	12	theme	healthy	529:535	arg1	women					546:550	healthy pregnant women	529:550	healthy pregnant women	529:550	Methods: Gut microbiota profiles of women with PE and healthy pregnant women in the third trimester and at 1 and 6 weeks postpartum were assessed by 16S rRNA gene amplicon sequencing.
31297341	9	13	from	consistent	1866:1875	arg1	terms					1748:1752	terms	1748:1752	terms of postpartum women	1748:1772	In terms of postpartum women, both the gut microbial composition and the PE-associated microbial alterations were highly consistent with those of the antepartum women.
31297341	9	14	theme	antepartum	1895:1904	arg1	women					1906:1910	the antepartum women	1891:1910	the antepartum women	1891:1910	In terms of postpartum women, both the gut microbial composition and the PE-associated microbial alterations were highly consistent with those of the antepartum women.
31297341	6	15	theme	antepartum	1222:1231	arg1	samples					1236:1242	antepartum PE samples	1222:1242	antepartum PE samples	1222:1242	Conversely, 5 genera, including Faecalibacterium, Gemmiger, Akkermansia, Dialister, and Methanobrevibacter, were significantly depleted in antepartum PE samples.
31297341	5	16	theme	healthy	952:958	arg1	controls					960:967	healthy controls	952:967	healthy controls	952:967	Results: At the genus level, 8 bacterial genera were significantly enriched in the antepartum samples of PE patients compared to healthy controls, of which Blautia, Ruminococcus2, Bilophila, and Fusobacterium represented the major variances in PE microbiomes.
31297341	7	17	theme	PE-depleted	1514:1524	arg1	genera					1526:1531	PE-depleted genera	1514:1531	PE-depleted genera	1514:1531	Maternal blood pressure and liver enzyme levels were positively correlated to the PE-enriched genera such as Anaerococcus, Ruminococcus2, Oribacterium, and Bilophila, while the fetal features (e.g., Apgar score and newborn birth weight) were positively correlated with PE-depleted genera and negatively correlated with PE-enriched genera.
31297341	7	18	theme	liver	1273:1277	arg1	levels					1286:1291	liver enzyme levels	1273:1291	liver enzyme levels	1273:1291	Maternal blood pressure and liver enzyme levels were positively correlated to the PE-enriched genera such as Anaerococcus, Ruminococcus2, Oribacterium, and Bilophila, while the fetal features (e.g., Apgar score and newborn birth weight) were positively correlated with PE-depleted genera and negatively correlated with PE-enriched genera.
31297341	10	19	theme	maternal	2102:2109	arg1	features					2120:2127	maternal clinical features	2102:2127	maternal clinical features (blood pressure level and liver dysfunction)	2102:2172	Conclusion: PE diagnosed in the third trimester of pregnancy is associated with a disrupted gut microbiota composition compared with uncomplicated pregnant women, which are associated with maternal clinical features (blood pressure level and liver dysfunction) and newborn birth weight.
31297341	10	19	theme	maternal	2102:2109	arg1	level					2145:2149	blood pressure level	2130:2149	blood pressure level	2130:2149	Conclusion: PE diagnosed in the third trimester of pregnancy is associated with a disrupted gut microbiota composition compared with uncomplicated pregnant women, which are associated with maternal clinical features (blood pressure level and liver dysfunction) and newborn birth weight.
31297341	10	19	theme	maternal	2102:2109	arg1	dysfunction					2161:2171	liver dysfunction	2155:2171	liver dysfunction	2155:2171	Conclusion: PE diagnosed in the third trimester of pregnancy is associated with a disrupted gut microbiota composition compared with uncomplicated pregnant women, which are associated with maternal clinical features (blood pressure level and liver dysfunction) and newborn birth weight.
31297341	4	20	theme	interleukin-6	676:688	arg1	levels					666:671	Plasma levels	659:671	Plasma levels of interleukin-6 (IL-6), intestinal fatty acid-binding protein (I-FABP), zonulin, and lipopolysaccharide (LPS)	659:782	Plasma levels of interleukin-6 (IL-6), intestinal fatty acid-binding protein (I-FABP), zonulin, and lipopolysaccharide (LPS) were measured in the third trimesters.
31297341	1	21	theme	microbiota	136:145	arg1	composition					147:157	gut microbiota composition	132:157	gut microbiota composition	132:157	Background: Imbalances in gut microbiota composition are linked to hypertension, host metabolic abnormalities, systemic inflammation, and other conditions.
31297341	1	22	theme	gut	132:134	arg1	composition					147:157	gut microbiota composition	132:157	gut microbiota composition	132:157	Background: Imbalances in gut microbiota composition are linked to hypertension, host metabolic abnormalities, systemic inflammation, and other conditions.
31297341	10	23	theme	microbiota	2009:2018	arg1	composition					2020:2030	a disrupted gut microbiota composition	1993:2030	a disrupted gut microbiota composition	1993:2030	Conclusion: PE diagnosed in the third trimester of pregnancy is associated with a disrupted gut microbiota composition compared with uncomplicated pregnant women, which are associated with maternal clinical features (blood pressure level and liver dysfunction) and newborn birth weight.
31297341	2	24	from	changes	300:306	arg1	women					329:333	women	329:333	women with early-onset preeclampsia (PE)	329:368	In the present study, we examined the changes of gut microbiota in women with early-onset preeclampsia (PE) and in normotensive, uncomplicated pregnant women during late pregnancy and at 1 and 6 weeks postpartum.
31297341	2	24	from	changes	300:306	arg1	women					414:418	normotensive, uncomplicated pregnant women	377:418	normotensive, uncomplicated pregnant women	377:418	In the present study, we examined the changes of gut microbiota in women with early-onset preeclampsia (PE) and in normotensive, uncomplicated pregnant women during late pregnancy and at 1 and 6 weeks postpartum.
31297341	10	25	theme	blood	2130:2134	arg1	features					2120:2127	maternal clinical features	2102:2127	maternal clinical features (blood pressure level and liver dysfunction)	2102:2172	Conclusion: PE diagnosed in the third trimester of pregnancy is associated with a disrupted gut microbiota composition compared with uncomplicated pregnant women, which are associated with maternal clinical features (blood pressure level and liver dysfunction) and newborn birth weight.
31297341	10	25	theme	blood	2130:2134	arg1	level					2145:2149	blood pressure level	2130:2149	blood pressure level	2130:2149	Conclusion: PE diagnosed in the third trimester of pregnancy is associated with a disrupted gut microbiota composition compared with uncomplicated pregnant women, which are associated with maternal clinical features (blood pressure level and liver dysfunction) and newborn birth weight.
31297341	4	26	theme	fatty	709:713	arg1	I-FABP					737:742	I-FABP	737:742	I-FABP	737:742	Plasma levels of interleukin-6 (IL-6), intestinal fatty acid-binding protein (I-FABP), zonulin, and lipopolysaccharide (LPS) were measured in the third trimesters.
31297341	4	26	theme	fatty	709:713	arg1	protein					728:734	intestinal fatty acid-binding protein	698:734	intestinal fatty acid-binding protein (I-FABP)	698:743	Plasma levels of interleukin-6 (IL-6), intestinal fatty acid-binding protein (I-FABP), zonulin, and lipopolysaccharide (LPS) were measured in the third trimesters.
31297341	10	27	theme	pregnancy	1964:1972	arg1	trimester					1951:1959	the third trimester	1941:1959	the third trimester of pregnancy	1941:1972	Conclusion: PE diagnosed in the third trimester of pregnancy is associated with a disrupted gut microbiota composition compared with uncomplicated pregnant women, which are associated with maternal clinical features (blood pressure level and liver dysfunction) and newborn birth weight.
31297341	7	28	theme	newborn	1460:1466	arg1	features					1428:1435	the fetal features	1418:1435	the fetal features (e.g., Apgar score and newborn birth weight)	1418:1480	Maternal blood pressure and liver enzyme levels were positively correlated to the PE-enriched genera such as Anaerococcus, Ruminococcus2, Oribacterium, and Bilophila, while the fetal features (e.g., Apgar score and newborn birth weight) were positively correlated with PE-depleted genera and negatively correlated with PE-enriched genera.
31297341	7	28	theme	newborn	1460:1466	arg1	weight					1474:1479	newborn birth weight	1460:1479	newborn birth weight	1460:1479	Maternal blood pressure and liver enzyme levels were positively correlated to the PE-enriched genera such as Anaerococcus, Ruminococcus2, Oribacterium, and Bilophila, while the fetal features (e.g., Apgar score and newborn birth weight) were positively correlated with PE-depleted genera and negatively correlated with PE-enriched genera.
31297341	10	29	theme	liver	2155:2159	arg1	features					2120:2127	maternal clinical features	2102:2127	maternal clinical features (blood pressure level and liver dysfunction)	2102:2172	Conclusion: PE diagnosed in the third trimester of pregnancy is associated with a disrupted gut microbiota composition compared with uncomplicated pregnant women, which are associated with maternal clinical features (blood pressure level and liver dysfunction) and newborn birth weight.
31297341	10	29	theme	liver	2155:2159	arg1	dysfunction					2161:2171	liver dysfunction	2155:2171	liver dysfunction	2155:2171	Conclusion: PE diagnosed in the third trimester of pregnancy is associated with a disrupted gut microbiota composition compared with uncomplicated pregnant women, which are associated with maternal clinical features (blood pressure level and liver dysfunction) and newborn birth weight.
31297341	4	30	theme	protein	728:734	arg1	levels					666:671	Plasma levels	659:671	Plasma levels of interleukin-6 (IL-6), intestinal fatty acid-binding protein (I-FABP), zonulin, and lipopolysaccharide (LPS)	659:782	Plasma levels of interleukin-6 (IL-6), intestinal fatty acid-binding protein (I-FABP), zonulin, and lipopolysaccharide (LPS) were measured in the third trimesters.
31297341	2	31	theme	normotensive	377:388	arg1	women					414:418	normotensive, uncomplicated pregnant women	377:418	normotensive, uncomplicated pregnant women	377:418	In the present study, we examined the changes of gut microbiota in women with early-onset preeclampsia (PE) and in normotensive, uncomplicated pregnant women during late pregnancy and at 1 and 6 weeks postpartum.
31297341	7	32	theme	blood	1254:1258	arg1	pressure					1260:1267	Maternal blood pressure	1245:1267	Maternal blood pressure	1245:1267	Maternal blood pressure and liver enzyme levels were positively correlated to the PE-enriched genera such as Anaerococcus, Ruminococcus2, Oribacterium, and Bilophila, while the fetal features (e.g., Apgar score and newborn birth weight) were positively correlated with PE-depleted genera and negatively correlated with PE-enriched genera.
31297341	0	33	theme	Early-Onset	0:10	arg1	Preeclampsia					12:23	Early-Onset Preeclampsia	0:23	Early-Onset Preeclampsia	0:23	Early-Onset Preeclampsia Is Associated With Gut Microbial Alterations in Antepartum and Postpartum Women.
31297341	1	34	from	Imbalances	118:127	arg1	composition					147:157	gut microbiota composition	132:157	gut microbiota composition	132:157	Background: Imbalances in gut microbiota composition are linked to hypertension, host metabolic abnormalities, systemic inflammation, and other conditions.
31297341	2	35	theme	late	427:430	arg1	pregnancy					432:440	late pregnancy	427:440	late pregnancy	427:440	In the present study, we examined the changes of gut microbiota in women with early-onset preeclampsia (PE) and in normotensive, uncomplicated pregnant women during late pregnancy and at 1 and 6 weeks postpartum.
31297341	10	36	theme	newborn	2178:2184	arg1	weight					2192:2197	newborn birth weight	2178:2197	newborn birth weight	2178:2197	Conclusion: PE diagnosed in the third trimester of pregnancy is associated with a disrupted gut microbiota composition compared with uncomplicated pregnant women, which are associated with maternal clinical features (blood pressure level and liver dysfunction) and newborn birth weight.
31297341	3	37	with	women	546:550	arg1	PE					522:523	PE	522:523	PE	522:523	Methods: Gut microbiota profiles of women with PE and healthy pregnant women in the third trimester and at 1 and 6 weeks postpartum were assessed by 16S rRNA gene amplicon sequencing.
31297341	7	38	theme	Apgar	1444:1448	arg1	features					1428:1435	the fetal features	1418:1435	the fetal features (e.g., Apgar score and newborn birth weight)	1418:1480	Maternal blood pressure and liver enzyme levels were positively correlated to the PE-enriched genera such as Anaerococcus, Ruminococcus2, Oribacterium, and Bilophila, while the fetal features (e.g., Apgar score and newborn birth weight) were positively correlated with PE-depleted genera and negatively correlated with PE-enriched genera.
31297341	7	38	theme	Apgar	1444:1448	arg1	score					1450:1454	Apgar score	1444:1454	Apgar score	1444:1454	Maternal blood pressure and liver enzyme levels were positively correlated to the PE-enriched genera such as Anaerococcus, Ruminococcus2, Oribacterium, and Bilophila, while the fetal features (e.g., Apgar score and newborn birth weight) were positively correlated with PE-depleted genera and negatively correlated with PE-enriched genera.
31297341	3	39	with	women	511:515	arg1	PE					522:523	PE	522:523	PE	522:523	Methods: Gut microbiota profiles of women with PE and healthy pregnant women in the third trimester and at 1 and 6 weeks postpartum were assessed by 16S rRNA gene amplicon sequencing.
31297341	5	40	theme	patients	931:938	arg1	samples					917:923	the antepartum samples	902:923	the antepartum samples of PE patients	902:938	Results: At the genus level, 8 bacterial genera were significantly enriched in the antepartum samples of PE patients compared to healthy controls, of which Blautia, Ruminococcus2, Bilophila, and Fusobacterium represented the major variances in PE microbiomes.
31297341	9	41	theme	women	1768:1772	arg1	terms					1748:1752	terms	1748:1752	terms of postpartum women	1748:1772	In terms of postpartum women, both the gut microbial composition and the PE-associated microbial alterations were highly consistent with those of the antepartum women.
31297341	3	42	theme	weeks	590:594	arg1	postpartum					596:605	1 and 6 weeks postpartum	582:605	1 and 6 weeks postpartum	582:605	Methods: Gut microbiota profiles of women with PE and healthy pregnant women in the third trimester and at 1 and 6 weeks postpartum were assessed by 16S rRNA gene amplicon sequencing.
31297341	4	43	theme	lipopolysaccharide	759:776	arg1	levels					666:671	Plasma levels	659:671	Plasma levels of interleukin-6 (IL-6), intestinal fatty acid-binding protein (I-FABP), zonulin, and lipopolysaccharide (LPS)	659:782	Plasma levels of interleukin-6 (IL-6), intestinal fatty acid-binding protein (I-FABP), zonulin, and lipopolysaccharide (LPS) were measured in the third trimesters.
31297341	0	44	theme	Microbial	48:56	arg1	Alterations					58:68	Gut Microbial Alterations	44:68	Gut Microbial Alterations in Antepartum and Postpartum Women	44:103	Early-Onset Preeclampsia Is Associated With Gut Microbial Alterations in Antepartum and Postpartum Women.
31297341	5	45	theme	PE	1067:1068	arg1	microbiomes					1070:1080	PE microbiomes	1067:1080	PE microbiomes	1067:1080	Results: At the genus level, 8 bacterial genera were significantly enriched in the antepartum samples of PE patients compared to healthy controls, of which Blautia, Ruminococcus2, Bilophila, and Fusobacterium represented the major variances in PE microbiomes.
31297341	10	46	theme	gut	2005:2007	arg1	composition					2020:2030	a disrupted gut microbiota composition	1993:2030	a disrupted gut microbiota composition	1993:2030	Conclusion: PE diagnosed in the third trimester of pregnancy is associated with a disrupted gut microbiota composition compared with uncomplicated pregnant women, which are associated with maternal clinical features (blood pressure level and liver dysfunction) and newborn birth weight.
31297341	3	47	theme	rRNA	628:631	arg1	sequencing					647:656	rRNA gene amplicon sequencing	628:656	rRNA gene amplicon sequencing	628:656	Methods: Gut microbiota profiles of women with PE and healthy pregnant women in the third trimester and at 1 and 6 weeks postpartum were assessed by 16S rRNA gene amplicon sequencing.
31297341	5	48	theme	antepartum	906:915	arg1	samples					917:923	the antepartum samples	902:923	the antepartum samples of PE patients	902:938	Results: At the genus level, 8 bacterial genera were significantly enriched in the antepartum samples of PE patients compared to healthy controls, of which Blautia, Ruminococcus2, Bilophila, and Fusobacterium represented the major variances in PE microbiomes.
31297341	9	49	theme	gut	1784:1786	arg1	consistent					1866:1875	consistent	1866:1875	consistent	1866:1875	In terms of postpartum women, both the gut microbial composition and the PE-associated microbial alterations were highly consistent with those of the antepartum women.
31297341	9	49	theme	gut	1784:1786	arg1	composition					1798:1808	the gut microbial composition	1780:1808	the gut microbial composition	1780:1808	In terms of postpartum women, both the gut microbial composition and the PE-associated microbial alterations were highly consistent with those of the antepartum women.
31297341	2	50	dep	normotensive	377:388	arg1	uncomplicated					391:403	uncomplicated	391:403	uncomplicated	391:403	In the present study, we examined the changes of gut microbiota in women with early-onset preeclampsia (PE) and in normotensive, uncomplicated pregnant women during late pregnancy and at 1 and 6 weeks postpartum.
31297341	3	51	theme	microbiota	488:497	arg1	profiles					499:506	Gut microbiota profiles	484:506	Gut microbiota profiles of women with PE and healthy pregnant women in the third trimester and at 1 and 6 weeks postpartum	484:605	Methods: Gut microbiota profiles of women with PE and healthy pregnant women in the third trimester and at 1 and 6 weeks postpartum were assessed by 16S rRNA gene amplicon sequencing.
31297341	1	52	theme	other	244:248	arg1	conditions					250:259	other conditions	244:259	other conditions	244:259	Background: Imbalances in gut microbiota composition are linked to hypertension, host metabolic abnormalities, systemic inflammation, and other conditions.
31297341	8	53	theme	maternal	1594:1601	arg1	level					1614:1618	maternal blood IL-6 level	1594:1618	maternal blood IL-6 level	1594:1618	Moreover, maternal blood IL-6 level was positively associated with gut Bilophila and Oribacterium, whereas LPS level was negatively associated with Akkermansia.
31297341	3	54	theme	amplicon	638:645	arg1	sequencing					647:656	rRNA gene amplicon sequencing	628:656	rRNA gene amplicon sequencing	628:656	Methods: Gut microbiota profiles of women with PE and healthy pregnant women in the third trimester and at 1 and 6 weeks postpartum were assessed by 16S rRNA gene amplicon sequencing.
31297341	5	55	from	variances	1054:1062	arg1	microbiomes					1070:1080	PE microbiomes	1067:1080	PE microbiomes	1067:1080	Results: At the genus level, 8 bacterial genera were significantly enriched in the antepartum samples of PE patients compared to healthy controls, of which Blautia, Ruminococcus2, Bilophila, and Fusobacterium represented the major variances in PE microbiomes.
31297341	8	56	theme	IL-6	1609:1612	arg1	level					1614:1618	maternal blood IL-6 level	1594:1618	maternal blood IL-6 level	1594:1618	Moreover, maternal blood IL-6 level was positively associated with gut Bilophila and Oribacterium, whereas LPS level was negatively associated with Akkermansia.
31297341	7	57	dep	features	1428:1435	arg1	features					1428:1435	the fetal features	1418:1435	the fetal features (e.g., Apgar score and newborn birth weight)	1418:1480	Maternal blood pressure and liver enzyme levels were positively correlated to the PE-enriched genera such as Anaerococcus, Ruminococcus2, Oribacterium, and Bilophila, while the fetal features (e.g., Apgar score and newborn birth weight) were positively correlated with PE-depleted genera and negatively correlated with PE-enriched genera.
31297341	7	57	dep	features	1428:1435	arg1	weight					1474:1479	newborn birth weight	1460:1479	newborn birth weight	1460:1479	Maternal blood pressure and liver enzyme levels were positively correlated to the PE-enriched genera such as Anaerococcus, Ruminococcus2, Oribacterium, and Bilophila, while the fetal features (e.g., Apgar score and newborn birth weight) were positively correlated with PE-depleted genera and negatively correlated with PE-enriched genera.
31297341	7	57	dep	features	1428:1435	arg1	score					1450:1454	Apgar score	1444:1454	Apgar score	1444:1454	Maternal blood pressure and liver enzyme levels were positively correlated to the PE-enriched genera such as Anaerococcus, Ruminococcus2, Oribacterium, and Bilophila, while the fetal features (e.g., Apgar score and newborn birth weight) were positively correlated with PE-depleted genera and negatively correlated with PE-enriched genera.
31297341	2	58	theme	gut	311:313	arg1	microbiota					315:324	gut microbiota	311:324	gut microbiota	311:324	In the present study, we examined the changes of gut microbiota in women with early-onset preeclampsia (PE) and in normotensive, uncomplicated pregnant women during late pregnancy and at 1 and 6 weeks postpartum.
31297341	9	59	theme	microbial	1832:1840	arg1	alterations					1842:1852	the PE-associated microbial alterations	1814:1852	the PE-associated microbial alterations	1814:1852	In terms of postpartum women, both the gut microbial composition and the PE-associated microbial alterations were highly consistent with those of the antepartum women.
31297341	9	59	theme	microbial	1832:1840	arg1	consistent					1866:1875	consistent	1866:1875	consistent	1866:1875	In terms of postpartum women, both the gut microbial composition and the PE-associated microbial alterations were highly consistent with those of the antepartum women.
31297341	3	60	from	postpartum	596:605	arg1	profiles					499:506	Gut microbiota profiles	484:506	Gut microbiota profiles of women with PE and healthy pregnant women in the third trimester and at 1 and 6 weeks postpartum	484:605	Methods: Gut microbiota profiles of women with PE and healthy pregnant women in the third trimester and at 1 and 6 weeks postpartum were assessed by 16S rRNA gene amplicon sequencing.
31297341	3	61	from	profiles	499:506	arg1	trimester					565:573	the third trimester	555:573	the third trimester	555:573	Methods: Gut microbiota profiles of women with PE and healthy pregnant women in the third trimester and at 1 and 6 weeks postpartum were assessed by 16S rRNA gene amplicon sequencing.
31297341	10	62	theme	pregnant	2060:2067	arg1	women					2069:2073	uncomplicated pregnant women	2046:2073	uncomplicated pregnant women	2046:2073	Conclusion: PE diagnosed in the third trimester of pregnancy is associated with a disrupted gut microbiota composition compared with uncomplicated pregnant women, which are associated with maternal clinical features (blood pressure level and liver dysfunction) and newborn birth weight.
31297341	1	63	attach	linked	163:168	arg2	Imbalances					118:127	Imbalances	118:127	Imbalances in gut microbiota composition	118:157	Background: Imbalances in gut microbiota composition are linked to hypertension, host metabolic abnormalities, systemic inflammation, and other conditions.
31297341	1	63	attach	linked	163:168	arg1	hypertension					173:184	hypertension	173:184	hypertension	173:184	Background: Imbalances in gut microbiota composition are linked to hypertension, host metabolic abnormalities, systemic inflammation, and other conditions.
31297341	1	63	attach	linked	163:168	arg1	conditions					250:259	other conditions	244:259	other conditions	244:259	Background: Imbalances in gut microbiota composition are linked to hypertension, host metabolic abnormalities, systemic inflammation, and other conditions.
31297341	1	63	attach	linked	163:168	arg1	abnormalities					202:214	host metabolic abnormalities	187:214	host metabolic abnormalities	187:214	Background: Imbalances in gut microbiota composition are linked to hypertension, host metabolic abnormalities, systemic inflammation, and other conditions.
31297341	1	63	attach	linked	163:168	arg1	inflammation					226:237	systemic inflammation	217:237	systemic inflammation	217:237	Background: Imbalances in gut microbiota composition are linked to hypertension, host metabolic abnormalities, systemic inflammation, and other conditions.
31297341	3	64	theme	pregnant	537:544	arg1	women					546:550	healthy pregnant women	529:550	healthy pregnant women	529:550	Methods: Gut microbiota profiles of women with PE and healthy pregnant women in the third trimester and at 1 and 6 weeks postpartum were assessed by 16S rRNA gene amplicon sequencing.
31297341	1	65	theme	host	187:190	arg1	abnormalities					202:214	host metabolic abnormalities	187:214	host metabolic abnormalities	187:214	Background: Imbalances in gut microbiota composition are linked to hypertension, host metabolic abnormalities, systemic inflammation, and other conditions.
31297341	11	66	theme	gut	2242:2244	arg1	microbiota					2246:2255	gut microbiota	2242:2255	gut microbiota	2242:2255	Moreover, these antepartum alterations in gut microbiota persisted 6 weeks postpartum.
31297341	8	67	theme	gut	1651:1653	arg1	Bilophila					1655:1663	gut Bilophila	1651:1663	gut Bilophila	1651:1663	Moreover, maternal blood IL-6 level was positively associated with gut Bilophila and Oribacterium, whereas LPS level was negatively associated with Akkermansia.
31297341	3	68	theme	third	559:563	arg1	trimester					565:573	the third trimester	555:573	the third trimester	555:573	Methods: Gut microbiota profiles of women with PE and healthy pregnant women in the third trimester and at 1 and 6 weeks postpartum were assessed by 16S rRNA gene amplicon sequencing.
31297341	1	69	theme	systemic	217:224	arg1	inflammation					226:237	systemic inflammation	217:237	systemic inflammation	217:237	Background: Imbalances in gut microbiota composition are linked to hypertension, host metabolic abnormalities, systemic inflammation, and other conditions.
31297341	6	70	theme	PE	1233:1234	arg1	samples					1236:1242	antepartum PE samples	1222:1242	antepartum PE samples	1222:1242	Conversely, 5 genera, including Faecalibacterium, Gemmiger, Akkermansia, Dialister, and Methanobrevibacter, were significantly depleted in antepartum PE samples.
31297341	10	71	theme	clinical	2111:2118	arg1	features					2120:2127	maternal clinical features	2102:2127	maternal clinical features (blood pressure level and liver dysfunction)	2102:2172	Conclusion: PE diagnosed in the third trimester of pregnancy is associated with a disrupted gut microbiota composition compared with uncomplicated pregnant women, which are associated with maternal clinical features (blood pressure level and liver dysfunction) and newborn birth weight.
31297341	10	71	theme	clinical	2111:2118	arg1	level					2145:2149	blood pressure level	2130:2149	blood pressure level	2130:2149	Conclusion: PE diagnosed in the third trimester of pregnancy is associated with a disrupted gut microbiota composition compared with uncomplicated pregnant women, which are associated with maternal clinical features (blood pressure level and liver dysfunction) and newborn birth weight.
31297341	10	71	theme	clinical	2111:2118	arg1	dysfunction					2161:2171	liver dysfunction	2155:2171	liver dysfunction	2155:2171	Conclusion: PE diagnosed in the third trimester of pregnancy is associated with a disrupted gut microbiota composition compared with uncomplicated pregnant women, which are associated with maternal clinical features (blood pressure level and liver dysfunction) and newborn birth weight.
31297341	4	72	theme	Plasma	659:664	arg1	levels					666:671	Plasma levels	659:671	Plasma levels of interleukin-6 (IL-6), intestinal fatty acid-binding protein (I-FABP), zonulin, and lipopolysaccharide (LPS)	659:782	Plasma levels of interleukin-6 (IL-6), intestinal fatty acid-binding protein (I-FABP), zonulin, and lipopolysaccharide (LPS) were measured in the third trimesters.
31297341	2	73	theme	present	269:275	arg1	study					277:281	the present study	265:281	the present study	265:281	In the present study, we examined the changes of gut microbiota in women with early-onset preeclampsia (PE) and in normotensive, uncomplicated pregnant women during late pregnancy and at 1 and 6 weeks postpartum.
31297341	8	74	theme	LPS	1691:1693	arg1	level					1695:1699	LPS level	1691:1699	LPS level	1691:1699	Moreover, maternal blood IL-6 level was positively associated with gut Bilophila and Oribacterium, whereas LPS level was negatively associated with Akkermansia.
31297341	5	75	theme	genus	839:843	arg1	level					845:849	the genus level	835:849	the genus level	835:849	Results: At the genus level, 8 bacterial genera were significantly enriched in the antepartum samples of PE patients compared to healthy controls, of which Blautia, Ruminococcus2, Bilophila, and Fusobacterium represented the major variances in PE microbiomes.
31297341	7	76	theme	enzyme	1279:1284	arg1	levels					1286:1291	liver enzyme levels	1273:1291	liver enzyme levels	1273:1291	Maternal blood pressure and liver enzyme levels were positively correlated to the PE-enriched genera such as Anaerococcus, Ruminococcus2, Oribacterium, and Bilophila, while the fetal features (e.g., Apgar score and newborn birth weight) were positively correlated with PE-depleted genera and negatively correlated with PE-enriched genera.
31297341	10	77	theme	third	1945:1949	arg1	trimester					1951:1959	the third trimester	1941:1959	the third trimester of pregnancy	1941:1972	Conclusion: PE diagnosed in the third trimester of pregnancy is associated with a disrupted gut microbiota composition compared with uncomplicated pregnant women, which are associated with maternal clinical features (blood pressure level and liver dysfunction) and newborn birth weight.
31297341	0	78	theme	Antepartum	73:82	arg1	Women					99:103	Antepartum and Postpartum Women	73:103	Antepartum and Postpartum Women	73:103	Early-Onset Preeclampsia Is Associated With Gut Microbial Alterations in Antepartum and Postpartum Women.
31297341	11	79	theme	postpartum	2275:2284	arg1	weeks					2269:2273	6 weeks postpartum	2267:2284	6 weeks postpartum	2267:2284	Moreover, these antepartum alterations in gut microbiota persisted 6 weeks postpartum.
31297341	10	80	theme	pressure	2136:2143	arg1	features					2120:2127	maternal clinical features	2102:2127	maternal clinical features (blood pressure level and liver dysfunction)	2102:2172	Conclusion: PE diagnosed in the third trimester of pregnancy is associated with a disrupted gut microbiota composition compared with uncomplicated pregnant women, which are associated with maternal clinical features (blood pressure level and liver dysfunction) and newborn birth weight.
31297341	10	80	theme	pressure	2136:2143	arg1	level					2145:2149	blood pressure level	2130:2149	blood pressure level	2130:2149	Conclusion: PE diagnosed in the third trimester of pregnancy is associated with a disrupted gut microbiota composition compared with uncomplicated pregnant women, which are associated with maternal clinical features (blood pressure level and liver dysfunction) and newborn birth weight.
31297341	0	81	theme	Postpartum	88:97	arg1	Women					99:103	Antepartum and Postpartum Women	73:103	Antepartum and Postpartum Women	73:103	Early-Onset Preeclampsia Is Associated With Gut Microbial Alterations in Antepartum and Postpartum Women.
31297341	7	82	theme	birth	1468:1472	arg1	features					1428:1435	the fetal features	1418:1435	the fetal features (e.g., Apgar score and newborn birth weight)	1418:1480	Maternal blood pressure and liver enzyme levels were positively correlated to the PE-enriched genera such as Anaerococcus, Ruminococcus2, Oribacterium, and Bilophila, while the fetal features (e.g., Apgar score and newborn birth weight) were positively correlated with PE-depleted genera and negatively correlated with PE-enriched genera.
31297341	7	82	theme	birth	1468:1472	arg1	weight					1474:1479	newborn birth weight	1460:1479	newborn birth weight	1460:1479	Maternal blood pressure and liver enzyme levels were positively correlated to the PE-enriched genera such as Anaerococcus, Ruminococcus2, Oribacterium, and Bilophila, while the fetal features (e.g., Apgar score and newborn birth weight) were positively correlated with PE-depleted genera and negatively correlated with PE-enriched genera.
31297341	2	83	theme	weeks	457:461	arg1	postpartum					463:472	1 and 6 weeks postpartum	449:472	1 and 6 weeks postpartum	449:472	In the present study, we examined the changes of gut microbiota in women with early-onset preeclampsia (PE) and in normotensive, uncomplicated pregnant women during late pregnancy and at 1 and 6 weeks postpartum.
31297341	5	84	theme	major	1048:1052	arg1	variances					1054:1062	the major variances	1044:1062	the major variances in PE microbiomes	1044:1080	Results: At the genus level, 8 bacterial genera were significantly enriched in the antepartum samples of PE patients compared to healthy controls, of which Blautia, Ruminococcus2, Bilophila, and Fusobacterium represented the major variances in PE microbiomes.
31297341	0	85	from	Alterations	58:68	arg1	Women					99:103	Antepartum and Postpartum Women	73:103	Antepartum and Postpartum Women	73:103	Early-Onset Preeclampsia Is Associated With Gut Microbial Alterations in Antepartum and Postpartum Women.
31297341	4	86	theme	intestinal	698:707	arg1	I-FABP					737:742	I-FABP	737:742	I-FABP	737:742	Plasma levels of interleukin-6 (IL-6), intestinal fatty acid-binding protein (I-FABP), zonulin, and lipopolysaccharide (LPS) were measured in the third trimesters.
31297341	4	86	theme	intestinal	698:707	arg1	protein					728:734	intestinal fatty acid-binding protein	698:734	intestinal fatty acid-binding protein (I-FABP)	698:743	Plasma levels of interleukin-6 (IL-6), intestinal fatty acid-binding protein (I-FABP), zonulin, and lipopolysaccharide (LPS) were measured in the third trimesters.
31297341	7	87	theme	Maternal	1245:1252	arg1	pressure					1260:1267	Maternal blood pressure	1245:1267	Maternal blood pressure	1245:1267	Maternal blood pressure and liver enzyme levels were positively correlated to the PE-enriched genera such as Anaerococcus, Ruminococcus2, Oribacterium, and Bilophila, while the fetal features (e.g., Apgar score and newborn birth weight) were positively correlated with PE-depleted genera and negatively correlated with PE-enriched genera.
31297341	4	88	theme	acid-binding	715:726	arg1	I-FABP					737:742	I-FABP	737:742	I-FABP	737:742	Plasma levels of interleukin-6 (IL-6), intestinal fatty acid-binding protein (I-FABP), zonulin, and lipopolysaccharide (LPS) were measured in the third trimesters.
31297341	4	88	theme	acid-binding	715:726	arg1	protein					728:734	intestinal fatty acid-binding protein	698:734	intestinal fatty acid-binding protein (I-FABP)	698:743	Plasma levels of interleukin-6 (IL-6), intestinal fatty acid-binding protein (I-FABP), zonulin, and lipopolysaccharide (LPS) were measured in the third trimesters.
31297341	10	89	theme	birth	2186:2190	arg1	weight					2192:2197	newborn birth weight	2178:2197	newborn birth weight	2178:2197	Conclusion: PE diagnosed in the third trimester of pregnancy is associated with a disrupted gut microbiota composition compared with uncomplicated pregnant women, which are associated with maternal clinical features (blood pressure level and liver dysfunction) and newborn birth weight.
31297341	5	90	theme	bacterial	854:862	arg1	genera					864:869	8 bacterial genera	852:869	8 bacterial genera	852:869	Results: At the genus level, 8 bacterial genera were significantly enriched in the antepartum samples of PE patients compared to healthy controls, of which Blautia, Ruminococcus2, Bilophila, and Fusobacterium represented the major variances in PE microbiomes.
31297341	11	91	from	alterations	2227:2237	arg1	microbiota					2246:2255	gut microbiota	2242:2255	gut microbiota	2242:2255	Moreover, these antepartum alterations in gut microbiota persisted 6 weeks postpartum.
31297341	2	92	theme	pregnant	405:412	arg1	women					414:418	normotensive, uncomplicated pregnant women	377:418	normotensive, uncomplicated pregnant women	377:418	In the present study, we examined the changes of gut microbiota in women with early-onset preeclampsia (PE) and in normotensive, uncomplicated pregnant women during late pregnancy and at 1 and 6 weeks postpartum.
31297341	0	93	theme	Gut	44:46	arg1	Alterations					58:68	Gut Microbial Alterations	44:68	Gut Microbial Alterations in Antepartum and Postpartum Women	44:103	Early-Onset Preeclampsia Is Associated With Gut Microbial Alterations in Antepartum and Postpartum Women.
31297341	5	94	theme	PE	928:929	arg1	patients					931:938	PE patients	928:938	PE patients	928:938	Results: At the genus level, 8 bacterial genera were significantly enriched in the antepartum samples of PE patients compared to healthy controls, of which Blautia, Ruminococcus2, Bilophila, and Fusobacterium represented the major variances in PE microbiomes.
31297341	10	95	dep	features	2120:2127	arg1	features					2120:2127	maternal clinical features	2102:2127	maternal clinical features (blood pressure level and liver dysfunction)	2102:2172	Conclusion: PE diagnosed in the third trimester of pregnancy is associated with a disrupted gut microbiota composition compared with uncomplicated pregnant women, which are associated with maternal clinical features (blood pressure level and liver dysfunction) and newborn birth weight.
31297341	10	95	dep	features	2120:2127	arg1	level					2145:2149	blood pressure level	2130:2149	blood pressure level	2130:2149	Conclusion: PE diagnosed in the third trimester of pregnancy is associated with a disrupted gut microbiota composition compared with uncomplicated pregnant women, which are associated with maternal clinical features (blood pressure level and liver dysfunction) and newborn birth weight.
31297341	10	95	dep	features	2120:2127	arg1	dysfunction					2161:2171	liver dysfunction	2155:2171	liver dysfunction	2155:2171	Conclusion: PE diagnosed in the third trimester of pregnancy is associated with a disrupted gut microbiota composition compared with uncomplicated pregnant women, which are associated with maternal clinical features (blood pressure level and liver dysfunction) and newborn birth weight.
31297341	9	96	theme	postpartum	1757:1766	arg1	women					1768:1772	postpartum women	1757:1772	postpartum women	1757:1772	In terms of postpartum women, both the gut microbial composition and the PE-associated microbial alterations were highly consistent with those of the antepartum women.
31297341	9	97	with	consistent	1866:1875	arg1	those					1882:1886	those	1882:1886	those	1882:1886	In terms of postpartum women, both the gut microbial composition and the PE-associated microbial alterations were highly consistent with those of the antepartum women.
31297341	4	98	theme	zonulin	746:752	arg1	levels					666:671	Plasma levels	659:671	Plasma levels of interleukin-6 (IL-6), intestinal fatty acid-binding protein (I-FABP), zonulin, and lipopolysaccharide (LPS)	659:782	Plasma levels of interleukin-6 (IL-6), intestinal fatty acid-binding protein (I-FABP), zonulin, and lipopolysaccharide (LPS) were measured in the third trimesters.
31297341	2	99	with	women	329:333	arg1	preeclampsia					352:363	early-onset preeclampsia	340:363	early-onset preeclampsia (PE)	340:368	In the present study, we examined the changes of gut microbiota in women with early-onset preeclampsia (PE) and in normotensive, uncomplicated pregnant women during late pregnancy and at 1 and 6 weeks postpartum.
31297341	2	99	with	women	329:333	arg1	PE					366:367	PE	366:367	PE	366:367	In the present study, we examined the changes of gut microbiota in women with early-onset preeclampsia (PE) and in normotensive, uncomplicated pregnant women during late pregnancy and at 1 and 6 weeks postpartum.
31297341	3	100	theme	Gut	484:486	arg1	profiles					499:506	Gut microbiota profiles	484:506	Gut microbiota profiles of women with PE and healthy pregnant women in the third trimester and at 1 and 6 weeks postpartum	484:605	Methods: Gut microbiota profiles of women with PE and healthy pregnant women in the third trimester and at 1 and 6 weeks postpartum were assessed by 16S rRNA gene amplicon sequencing.
31297341	10	101	theme	disrupted	1995:2003	arg1	composition					2020:2030	a disrupted gut microbiota composition	1993:2030	a disrupted gut microbiota composition	1993:2030	Conclusion: PE diagnosed in the third trimester of pregnancy is associated with a disrupted gut microbiota composition compared with uncomplicated pregnant women, which are associated with maternal clinical features (blood pressure level and liver dysfunction) and newborn birth weight.
31297341	2	102	theme	microbiota	315:324	arg1	changes					300:306	the changes	296:306	the changes of gut microbiota in women with early-onset preeclampsia (PE) and in normotensive, uncomplicated pregnant women	296:418	In the present study, we examined the changes of gut microbiota in women with early-onset preeclampsia (PE) and in normotensive, uncomplicated pregnant women during late pregnancy and at 1 and 6 weeks postpartum.
31297341	7	103	theme	fetal	1422:1426	arg1	features					1428:1435	the fetal features	1418:1435	the fetal features (e.g., Apgar score and newborn birth weight)	1418:1480	Maternal blood pressure and liver enzyme levels were positively correlated to the PE-enriched genera such as Anaerococcus, Ruminococcus2, Oribacterium, and Bilophila, while the fetal features (e.g., Apgar score and newborn birth weight) were positively correlated with PE-depleted genera and negatively correlated with PE-enriched genera.
31297341	7	103	theme	fetal	1422:1426	arg1	weight					1474:1479	newborn birth weight	1460:1479	newborn birth weight	1460:1479	Maternal blood pressure and liver enzyme levels were positively correlated to the PE-enriched genera such as Anaerococcus, Ruminococcus2, Oribacterium, and Bilophila, while the fetal features (e.g., Apgar score and newborn birth weight) were positively correlated with PE-depleted genera and negatively correlated with PE-enriched genera.
31297341	7	103	theme	fetal	1422:1426	arg1	score					1450:1454	Apgar score	1444:1454	Apgar score	1444:1454	Maternal blood pressure and liver enzyme levels were positively correlated to the PE-enriched genera such as Anaerococcus, Ruminococcus2, Oribacterium, and Bilophila, while the fetal features (e.g., Apgar score and newborn birth weight) were positively correlated with PE-depleted genera and negatively correlated with PE-enriched genera.
31297341	9	104	theme	microbial	1788:1796	arg1	consistent					1866:1875	consistent	1866:1875	consistent	1866:1875	In terms of postpartum women, both the gut microbial composition and the PE-associated microbial alterations were highly consistent with those of the antepartum women.
31297341	9	104	theme	microbial	1788:1796	arg1	composition					1798:1808	the gut microbial composition	1780:1808	the gut microbial composition	1780:1808	In terms of postpartum women, both the gut microbial composition and the PE-associated microbial alterations were highly consistent with those of the antepartum women.
31297341	8	105	theme	blood	1603:1607	arg1	level					1614:1618	maternal blood IL-6 level	1594:1618	maternal blood IL-6 level	1594:1618	Moreover, maternal blood IL-6 level was positively associated with gut Bilophila and Oribacterium, whereas LPS level was negatively associated with Akkermansia.
31297341	7	106	dep	score	1450:1454	arg1	e.g.					1438:1441	e.g.	1438:1441	e.g.	1438:1441	Maternal blood pressure and liver enzyme levels were positively correlated to the PE-enriched genera such as Anaerococcus, Ruminococcus2, Oribacterium, and Bilophila, while the fetal features (e.g., Apgar score and newborn birth weight) were positively correlated with PE-depleted genera and negatively correlated with PE-enriched genera.
31138200	11	0	theme	porous	1504:1509	arg1	candidate					1546:1554	a good candidate	1539:1554	a good candidate for a tissue engineering material in cartilage repair	1539:1608	CONCLUSIONS The novel PVA/CS porous composite hydrogel could be a good candidate for a tissue engineering material in cartilage repair.
31138200	11	0	theme	porous	1504:1509	arg1	hydrogel					1521:1528	The novel PVA/CS porous composite hydrogel	1487:1528	The novel PVA/CS porous composite hydrogel	1487:1528	CONCLUSIONS The novel PVA/CS porous composite hydrogel could be a good candidate for a tissue engineering material in cartilage repair.
31138200	6	1	theme	HG	899:900	arg1	group					892:896	hydrogel group	883:896	hydrogel group (HG group)	883:907	They were assigned to different groups randomly (n = 6 per group): the osteochondral defect only group (control group), the osteochondral defect treated with hydrogel group (HG group), and the osteochondral defect treated with hydrogel loaded with bone marrow mesenchymal stem cells (BMSCs) group (HG-BMSCs group).
31138200	6	1	theme	HG	899:900	arg1	group					902:906	HG group	899:906	HG group	899:906	They were assigned to different groups randomly (n = 6 per group): the osteochondral defect only group (control group), the osteochondral defect treated with hydrogel group (HG group), and the osteochondral defect treated with hydrogel loaded with bone marrow mesenchymal stem cells (BMSCs) group (HG-BMSCs group).
31138200	7	2	theme	macro-observation	1072:1088	arg1	evaluation					1107:1116	macro-observation and histological evaluation	1072:1116	macro-observation and histological evaluation at 12 weeks after surgery	1072:1142	The cartilage was collected for macro-observation and histological evaluation at 12 weeks after surgery.
31138200	11	3	theme	material	1581:1588	arg1	engineering					1569:1579	a tissue engineering	1560:1579	a tissue engineering material in cartilage repair	1560:1608	CONCLUSIONS The novel PVA/CS porous composite hydrogel could be a good candidate for a tissue engineering material in cartilage repair.
31138200	3	4	theme	mechanical	504:513	arg1	properties					515:524	physiochemical and mechanical properties	485:524	properties	515:524	METHODS Hydrogels of various PVA/CS ratios were tested for physiochemical and mechanical properties in addition to cytotoxicity and biocompatibility.
31138200	11	5	theme	novel	1491:1495	arg1	candidate					1546:1554	a good candidate	1539:1554	a good candidate for a tissue engineering material in cartilage repair	1539:1608	CONCLUSIONS The novel PVA/CS porous composite hydrogel could be a good candidate for a tissue engineering material in cartilage repair.
31138200	11	5	theme	novel	1491:1495	arg1	hydrogel					1521:1528	The novel PVA/CS porous composite hydrogel	1487:1528	The novel PVA/CS porous composite hydrogel	1487:1528	CONCLUSIONS The novel PVA/CS porous composite hydrogel could be a good candidate for a tissue engineering material in cartilage repair.
31138200	4	6	theme	best	599:602	arg1	ratio					611:615	the best PVA/CS ratio	595:615	the best PVA/CS ratio	595:615	The hydrogels with the best PVA/CS ratio were used in the animal study.
31138200	0	7	theme	bone	85:88	arg1	marrow					90:95	bone marrow mesenchymal stem cells and its application in articular cartilage repair	85:168	marrow	90:95	Characterization of a novel polyvinyl alcohol/chitosan porous hydrogel combined with bone marrow mesenchymal stem cells and its application in articular cartilage repair.
31138200	6	8	theme	only	817:820	arg1	group					822:826	the osteochondral defect only group	792:826	the osteochondral defect only group (control group)	792:842	They were assigned to different groups randomly (n = 6 per group): the osteochondral defect only group (control group), the osteochondral defect treated with hydrogel group (HG group), and the osteochondral defect treated with hydrogel loaded with bone marrow mesenchymal stem cells (BMSCs) group (HG-BMSCs group).
31138200	6	8	theme	only	817:820	arg1	group					837:841	control group	829:841	control group	829:841	They were assigned to different groups randomly (n = 6 per group): the osteochondral defect only group (control group), the osteochondral defect treated with hydrogel group (HG group), and the osteochondral defect treated with hydrogel loaded with bone marrow mesenchymal stem cells (BMSCs) group (HG-BMSCs group).
31138200	2	9	theme	porous	342:347	arg1	hydrogel					349:356	A novel Polyvinyl alcohol (PVA)/chitosan (CS) porous hydrogel	296:356	A novel Polyvinyl alcohol (PVA)/chitosan (CS) porous hydrogel	296:356	A novel Polyvinyl alcohol (PVA)/chitosan (CS) porous hydrogel was designed and applied to the treatment of osteochondral defects.
31138200	6	10	theme	osteochondral	849:861	arg1	defect					863:868	the osteochondral defect	845:868	the osteochondral defect treated with hydrogel group (HG group)	845:907	They were assigned to different groups randomly (n = 6 per group): the osteochondral defect only group (control group), the osteochondral defect treated with hydrogel group (HG group), and the osteochondral defect treated with hydrogel loaded with bone marrow mesenchymal stem cells (BMSCs) group (HG-BMSCs group).
31138200	0	11	theme	stem	109:112	arg1	cells					114:118	mesenchymal stem cells	97:118	mesenchymal stem cells	97:118	Characterization of a novel polyvinyl alcohol/chitosan porous hydrogel combined with bone marrow mesenchymal stem cells and its application in articular cartilage repair.
31138200	7	12	from	12 weeks	1121:1128	arg1	evaluation					1107:1116	macro-observation and histological evaluation	1072:1116	macro-observation and histological evaluation at 12 weeks after surgery	1072:1142	The cartilage was collected for macro-observation and histological evaluation at 12 weeks after surgery.
31138200	6	13	theme	defect	810:815	arg1	group					822:826	the osteochondral defect only group	792:826	the osteochondral defect only group (control group)	792:842	They were assigned to different groups randomly (n = 6 per group): the osteochondral defect only group (control group), the osteochondral defect treated with hydrogel group (HG group), and the osteochondral defect treated with hydrogel loaded with bone marrow mesenchymal stem cells (BMSCs) group (HG-BMSCs group).
31138200	6	13	theme	defect	810:815	arg1	group					837:841	control group	829:841	control group	829:841	They were assigned to different groups randomly (n = 6 per group): the osteochondral defect only group (control group), the osteochondral defect treated with hydrogel group (HG group), and the osteochondral defect treated with hydrogel loaded with bone marrow mesenchymal stem cells (BMSCs) group (HG-BMSCs group).
31138200	2	14	theme	Polyvinyl	304:312	arg1	alcohol					314:320	novel Polyvinyl alcohol	298:320	novel Polyvinyl alcohol	298:320	A novel Polyvinyl alcohol (PVA)/chitosan (CS) porous hydrogel was designed and applied to the treatment of osteochondral defects.
31138200	1	15	theme	appropriate	271:281	arg1	biomaterial					283:293	an appropriate biomaterial	268:293	an appropriate biomaterial	268:293	BACKGROUND Different substances are combined to compensate for each other's drawbacks and create an appropriate biomaterial.
31138200	0	16	theme	mesenchymal	97:107	arg1	cells					114:118	mesenchymal stem cells	97:118	mesenchymal stem cells	97:118	Characterization of a novel polyvinyl alcohol/chitosan porous hydrogel combined with bone marrow mesenchymal stem cells and its application in articular cartilage repair.
31138200	6	17	theme	hydrogel	883:890	arg1	group					892:896	hydrogel group	883:896	hydrogel group (HG group)	883:907	They were assigned to different groups randomly (n = 6 per group): the osteochondral defect only group (control group), the osteochondral defect treated with hydrogel group (HG group), and the osteochondral defect treated with hydrogel loaded with bone marrow mesenchymal stem cells (BMSCs) group (HG-BMSCs group).
31138200	6	17	theme	hydrogel	883:890	arg1	group					902:906	HG group	899:906	HG group	899:906	They were assigned to different groups randomly (n = 6 per group): the osteochondral defect only group (control group), the osteochondral defect treated with hydrogel group (HG group), and the osteochondral defect treated with hydrogel loaded with bone marrow mesenchymal stem cells (BMSCs) group (HG-BMSCs group).
31138200	2	18	theme	novel	298:302	arg1	alcohol					314:320	novel Polyvinyl alcohol	298:320	novel Polyvinyl alcohol	298:320	A novel Polyvinyl alcohol (PVA)/chitosan (CS) porous hydrogel was designed and applied to the treatment of osteochondral defects.
31138200	6	19	theme	osteochondral	796:808	arg1	group					822:826	the osteochondral defect only group	792:826	the osteochondral defect only group (control group)	792:842	They were assigned to different groups randomly (n = 6 per group): the osteochondral defect only group (control group), the osteochondral defect treated with hydrogel group (HG group), and the osteochondral defect treated with hydrogel loaded with bone marrow mesenchymal stem cells (BMSCs) group (HG-BMSCs group).
31138200	6	19	theme	osteochondral	796:808	arg1	group					837:841	control group	829:841	control group	829:841	They were assigned to different groups randomly (n = 6 per group): the osteochondral defect only group (control group), the osteochondral defect treated with hydrogel group (HG group), and the osteochondral defect treated with hydrogel loaded with bone marrow mesenchymal stem cells (BMSCs) group (HG-BMSCs group).
31138200	2	20	dep	porous	342:347	arg1	alcohol					314:320	novel Polyvinyl alcohol	298:320	novel Polyvinyl alcohol	298:320	A novel Polyvinyl alcohol (PVA)/chitosan (CS) porous hydrogel was designed and applied to the treatment of osteochondral defects.
31138200	4	21	theme	PVA/CS	604:609	arg1	ratio					611:615	the best PVA/CS ratio	595:615	the best PVA/CS ratio	595:615	The hydrogels with the best PVA/CS ratio were used in the animal study.
31138200	8	22	theme	physical	1255:1262	arg1	properties					1277:1286	stable physical and chemical properties	1248:1286	stable physical and chemical properties	1248:1286	RESULTS The Hydrogel with PVA/CS ratio of 6:4 exhibited the best mechanical properties; it also showed stable physical and chemical properties with porosity and over 90% water content.
31138200	4	23	theme	animal	634:639	arg1	study					641:645	the animal study	630:645	the animal study	630:645	The hydrogels with the best PVA/CS ratio were used in the animal study.
31138200	8	24	theme	water	1315:1319	arg1	content					1321:1327	over 90% water content	1306:1327	over 90% water content	1306:1327	RESULTS The Hydrogel with PVA/CS ratio of 6:4 exhibited the best mechanical properties; it also showed stable physical and chemical properties with porosity and over 90% water content.
31138200	1	25	theme	Different	182:190	arg1	substances					192:201	BACKGROUND Different substances	171:201	BACKGROUND Different substances	171:201	BACKGROUND Different substances are combined to compensate for each other's drawbacks and create an appropriate biomaterial.
31138200	8	26	theme	6:4	1187:1189	arg1	ratio					1178:1182	PVA/CS ratio	1171:1182	PVA/CS ratio of 6:4	1171:1189	RESULTS The Hydrogel with PVA/CS ratio of 6:4 exhibited the best mechanical properties; it also showed stable physical and chemical properties with porosity and over 90% water content.
31138200	4	27	with	hydrogels	580:588	arg1	ratio					611:615	the best PVA/CS ratio	595:615	the best PVA/CS ratio	595:615	The hydrogels with the best PVA/CS ratio were used in the animal study.
31138200	6	28	theme	bone	973:976	arg1	BMSCs					1009:1013	BMSCs	1009:1013	BMSCs	1009:1013	They were assigned to different groups randomly (n = 6 per group): the osteochondral defect only group (control group), the osteochondral defect treated with hydrogel group (HG group), and the osteochondral defect treated with hydrogel loaded with bone marrow mesenchymal stem cells (BMSCs) group (HG-BMSCs group).
31138200	6	28	theme	bone	973:976	arg1	cells					1002:1006	bone marrow mesenchymal stem cells	973:1006	bone marrow mesenchymal stem cells (BMSCs) group (HG-BMSCs group)	973:1037	They were assigned to different groups randomly (n = 6 per group): the osteochondral defect only group (control group), the osteochondral defect treated with hydrogel group (HG group), and the osteochondral defect treated with hydrogel loaded with bone marrow mesenchymal stem cells (BMSCs) group (HG-BMSCs group).
31138200	9	29	theme	cell	1399:1402	arg1	proliferation					1404:1416	cell proliferation	1399:1416	cell proliferation	1399:1416	Furthermore, it demonstrated no cytotoxicity and was able to promote cell proliferation.
31138200	8	30	dep	content	1321:1327	arg1	%					1313:1313	%	1313:1313	%	1313:1313	RESULTS The Hydrogel with PVA/CS ratio of 6:4 exhibited the best mechanical properties; it also showed stable physical and chemical properties with porosity and over 90% water content.
31138200	10	31	theme	HG-BMSCs	1423:1430	arg1	group					1432:1436	The HG-BMSCs group	1419:1436	The HG-BMSCs group	1419:1436	The HG-BMSCs group achieved the best cartilage healing.
31138200	11	32	theme	good	1541:1544	arg1	candidate					1546:1554	a good candidate	1539:1554	a good candidate for a tissue engineering material in cartilage repair	1539:1608	CONCLUSIONS The novel PVA/CS porous composite hydrogel could be a good candidate for a tissue engineering material in cartilage repair.
31138200	11	32	theme	good	1541:1544	arg1	hydrogel					1521:1528	The novel PVA/CS porous composite hydrogel	1487:1528	The novel PVA/CS porous composite hydrogel	1487:1528	CONCLUSIONS The novel PVA/CS porous composite hydrogel could be a good candidate for a tissue engineering material in cartilage repair.
31138200	0	33	dep	marrow	90:95	arg1	cells					114:118	mesenchymal stem cells	97:118	mesenchymal stem cells	97:118	Characterization of a novel polyvinyl alcohol/chitosan porous hydrogel combined with bone marrow mesenchymal stem cells and its application in articular cartilage repair.
31138200	5	34	theme	articular	690:698	arg1	cartilage					700:708	the articular cartilage	686:708	the articular cartilage of 18 rabbits	686:722	Osteochondral defects were created at the articular cartilage of 18 rabbits.
31138200	6	35	theme	HG-BMSCs	1023:1030	arg1	group					1016:1020	bone marrow mesenchymal stem cells (BMSCs) group	973:1020	bone marrow mesenchymal stem cells (BMSCs) group (HG-BMSCs group)	973:1037	They were assigned to different groups randomly (n = 6 per group): the osteochondral defect only group (control group), the osteochondral defect treated with hydrogel group (HG group), and the osteochondral defect treated with hydrogel loaded with bone marrow mesenchymal stem cells (BMSCs) group (HG-BMSCs group).
31138200	6	35	theme	HG-BMSCs	1023:1030	arg1	group					1032:1036	HG-BMSCs group	1023:1036	HG-BMSCs group	1023:1036	They were assigned to different groups randomly (n = 6 per group): the osteochondral defect only group (control group), the osteochondral defect treated with hydrogel group (HG group), and the osteochondral defect treated with hydrogel loaded with bone marrow mesenchymal stem cells (BMSCs) group (HG-BMSCs group).
31138200	10	36	theme	best	1451:1454	arg1	healing					1466:1472	the best cartilage healing	1447:1472	the best cartilage healing	1447:1472	The HG-BMSCs group achieved the best cartilage healing.
31138200	5	37	theme	Osteochondral	648:660	arg1	defects					662:668	Osteochondral defects	648:668	Osteochondral defects	648:668	Osteochondral defects were created at the articular cartilage of 18 rabbits.
31138200	3	38	theme	METHODS	426:432	arg1	Hydrogels					434:442	METHODS Hydrogels	426:442	METHODS Hydrogels of various PVA/CS ratios	426:467	METHODS Hydrogels of various PVA/CS ratios were tested for physiochemical and mechanical properties in addition to cytotoxicity and biocompatibility.
31138200	0	39	theme	novel	22:26	arg1	hydrogel					62:69	a novel polyvinyl alcohol/chitosan porous hydrogel	20:69	a novel polyvinyl alcohol/chitosan porous hydrogel	20:69	Characterization of a novel polyvinyl alcohol/chitosan porous hydrogel combined with bone marrow mesenchymal stem cells and its application in articular cartilage repair.
31138200	8	40	theme	PVA/CS	1171:1176	arg1	ratio					1178:1182	PVA/CS ratio	1171:1182	PVA/CS ratio of 6:4	1171:1189	RESULTS The Hydrogel with PVA/CS ratio of 6:4 exhibited the best mechanical properties; it also showed stable physical and chemical properties with porosity and over 90% water content.
31138200	11	41	theme	composite	1511:1519	arg1	candidate					1546:1554	a good candidate	1539:1554	a good candidate for a tissue engineering material in cartilage repair	1539:1608	CONCLUSIONS The novel PVA/CS porous composite hydrogel could be a good candidate for a tissue engineering material in cartilage repair.
31138200	11	41	theme	composite	1511:1519	arg1	hydrogel					1521:1528	The novel PVA/CS porous composite hydrogel	1487:1528	The novel PVA/CS porous composite hydrogel	1487:1528	CONCLUSIONS The novel PVA/CS porous composite hydrogel could be a good candidate for a tissue engineering material in cartilage repair.
31138200	2	42	theme	defects	417:423	arg1	treatment					390:398	the treatment	386:398	the treatment of osteochondral defects	386:423	A novel Polyvinyl alcohol (PVA)/chitosan (CS) porous hydrogel was designed and applied to the treatment of osteochondral defects.
31138200	11	43	theme	cartilage	1593:1601	arg1	repair					1603:1608	cartilage repair	1593:1608	cartilage repair	1593:1608	CONCLUSIONS The novel PVA/CS porous composite hydrogel could be a good candidate for a tissue engineering material in cartilage repair.
31138200	5	44	theme	rabbits	716:722	arg1	cartilage					700:708	the articular cartilage	686:708	the articular cartilage of 18 rabbits	686:722	Osteochondral defects were created at the articular cartilage of 18 rabbits.
31138200	0	45	from	marrow	90:95	arg1	repair					163:168	articular cartilage repair	143:168	articular cartilage repair	143:168	Characterization of a novel polyvinyl alcohol/chitosan porous hydrogel combined with bone marrow mesenchymal stem cells and its application in articular cartilage repair.
31138200	1	46	theme	BACKGROUND	171:180	arg1	substances					192:201	BACKGROUND Different substances	171:201	BACKGROUND Different substances	171:201	BACKGROUND Different substances are combined to compensate for each other's drawbacks and create an appropriate biomaterial.
31138200	2	47	theme	osteochondral	403:415	arg1	defects					417:423	osteochondral defects	403:423	osteochondral defects	403:423	A novel Polyvinyl alcohol (PVA)/chitosan (CS) porous hydrogel was designed and applied to the treatment of osteochondral defects.
31138200	0	48	theme	alcohol/chitosan	38:53	arg1	hydrogel					62:69	a novel polyvinyl alcohol/chitosan porous hydrogel	20:69	a novel polyvinyl alcohol/chitosan porous hydrogel	20:69	Characterization of a novel polyvinyl alcohol/chitosan porous hydrogel combined with bone marrow mesenchymal stem cells and its application in articular cartilage repair.
31138200	6	49	theme	osteochondral	918:930	arg1	defect					932:937	the osteochondral defect	914:937	the osteochondral defect	914:937	They were assigned to different groups randomly (n = 6 per group): the osteochondral defect only group (control group), the osteochondral defect treated with hydrogel group (HG group), and the osteochondral defect treated with hydrogel loaded with bone marrow mesenchymal stem cells (BMSCs) group (HG-BMSCs group).
31138200	0	50	theme	articular	143:151	arg1	repair					163:168	articular cartilage repair	143:168	articular cartilage repair	143:168	Characterization of a novel polyvinyl alcohol/chitosan porous hydrogel combined with bone marrow mesenchymal stem cells and its application in articular cartilage repair.
31138200	8	51	theme	mechanical	1210:1219	arg1	properties					1221:1230	the best mechanical properties	1201:1230	the best mechanical properties	1201:1230	RESULTS The Hydrogel with PVA/CS ratio of 6:4 exhibited the best mechanical properties; it also showed stable physical and chemical properties with porosity and over 90% water content.
31138200	3	52	theme	various	447:453	arg1	ratios					462:467	various PVA/CS ratios	447:467	various PVA/CS ratios	447:467	METHODS Hydrogels of various PVA/CS ratios were tested for physiochemical and mechanical properties in addition to cytotoxicity and biocompatibility.
31138200	0	53	theme	polyvinyl	28:36	arg1	hydrogel					62:69	a novel polyvinyl alcohol/chitosan porous hydrogel	20:69	a novel polyvinyl alcohol/chitosan porous hydrogel	20:69	Characterization of a novel polyvinyl alcohol/chitosan porous hydrogel combined with bone marrow mesenchymal stem cells and its application in articular cartilage repair.
31138200	8	54	dep	%	1313:1313	arg1	90					1311:1312	90	1311:1312	90	1311:1312	RESULTS The Hydrogel with PVA/CS ratio of 6:4 exhibited the best mechanical properties; it also showed stable physical and chemical properties with porosity and over 90% water content.
31138200	4	55	used	used	622:625	arg2	hydrogels					580:588	The hydrogels	576:588	The hydrogels with the best PVA/CS ratio	576:615	The hydrogels with the best PVA/CS ratio were used in the animal study.
31138200	6	56	theme	mesenchymal	985:995	arg1	BMSCs					1009:1013	BMSCs	1009:1013	BMSCs	1009:1013	They were assigned to different groups randomly (n = 6 per group): the osteochondral defect only group (control group), the osteochondral defect treated with hydrogel group (HG group), and the osteochondral defect treated with hydrogel loaded with bone marrow mesenchymal stem cells (BMSCs) group (HG-BMSCs group).
31138200	6	56	theme	mesenchymal	985:995	arg1	cells					1002:1006	bone marrow mesenchymal stem cells	973:1006	bone marrow mesenchymal stem cells (BMSCs) group (HG-BMSCs group)	973:1037	They were assigned to different groups randomly (n = 6 per group): the osteochondral defect only group (control group), the osteochondral defect treated with hydrogel group (HG group), and the osteochondral defect treated with hydrogel loaded with bone marrow mesenchymal stem cells (BMSCs) group (HG-BMSCs group).
31138200	3	57	theme	PVA/CS	455:460	arg1	ratios					462:467	various PVA/CS ratios	447:467	various PVA/CS ratios	447:467	METHODS Hydrogels of various PVA/CS ratios were tested for physiochemical and mechanical properties in addition to cytotoxicity and biocompatibility.
31138200	0	58	theme	hydrogel	62:69	arg1	Characterization					0:15	Characterization	0:15	Characterization of a novel polyvinyl alcohol/chitosan porous hydrogel combined with bone marrow mesenchymal stem cells and its application in articular cartilage repair.	0:169	Characterization of a novel polyvinyl alcohol/chitosan porous hydrogel combined with bone marrow mesenchymal stem cells and its application in articular cartilage repair.
31138200	11	59	dep	CONCLUSIONS	1475:1485	arg1	candidate					1546:1554	a good candidate	1539:1554	a good candidate for a tissue engineering material in cartilage repair	1539:1608	CONCLUSIONS The novel PVA/CS porous composite hydrogel could be a good candidate for a tissue engineering material in cartilage repair.
31138200	11	59	dep	CONCLUSIONS	1475:1485	arg1	hydrogel					1521:1528	The novel PVA/CS porous composite hydrogel	1487:1528	The novel PVA/CS porous composite hydrogel	1487:1528	CONCLUSIONS The novel PVA/CS porous composite hydrogel could be a good candidate for a tissue engineering material in cartilage repair.
31138200	11	60	theme	tissue	1562:1567	arg1	engineering					1569:1579	a tissue engineering	1560:1579	a tissue engineering material in cartilage repair	1560:1608	CONCLUSIONS The novel PVA/CS porous composite hydrogel could be a good candidate for a tissue engineering material in cartilage repair.
31138200	3	61	theme	physiochemical	485:498	arg1	properties					515:524	physiochemical and mechanical properties	485:524	properties	515:524	METHODS Hydrogels of various PVA/CS ratios were tested for physiochemical and mechanical properties in addition to cytotoxicity and biocompatibility.
31138200	0	62	theme	porous	55:60	arg1	hydrogel					62:69	a novel polyvinyl alcohol/chitosan porous hydrogel	20:69	a novel polyvinyl alcohol/chitosan porous hydrogel	20:69	Characterization of a novel polyvinyl alcohol/chitosan porous hydrogel combined with bone marrow mesenchymal stem cells and its application in articular cartilage repair.
31138200	0	63	theme	cartilage	153:161	arg1	repair					163:168	articular cartilage repair	143:168	articular cartilage repair	143:168	Characterization of a novel polyvinyl alcohol/chitosan porous hydrogel combined with bone marrow mesenchymal stem cells and its application in articular cartilage repair.
31138200	8	64	with	Hydrogel	1157:1164	arg1	ratio					1178:1182	PVA/CS ratio	1171:1182	PVA/CS ratio of 6:4	1171:1189	RESULTS The Hydrogel with PVA/CS ratio of 6:4 exhibited the best mechanical properties; it also showed stable physical and chemical properties with porosity and over 90% water content.
31138200	8	65	theme	stable	1248:1253	arg1	properties					1277:1286	stable physical and chemical properties	1248:1286	stable physical and chemical properties	1248:1286	RESULTS The Hydrogel with PVA/CS ratio of 6:4 exhibited the best mechanical properties; it also showed stable physical and chemical properties with porosity and over 90% water content.
31138200	11	66	from	repair	1603:1608	arg1	material					1581:1588	material	1581:1588	material	1581:1588	CONCLUSIONS The novel PVA/CS porous composite hydrogel could be a good candidate for a tissue engineering material in cartilage repair.
31138200	6	67	theme	control	829:835	arg1	group					822:826	the osteochondral defect only group	792:826	the osteochondral defect only group (control group)	792:842	They were assigned to different groups randomly (n = 6 per group): the osteochondral defect only group (control group), the osteochondral defect treated with hydrogel group (HG group), and the osteochondral defect treated with hydrogel loaded with bone marrow mesenchymal stem cells (BMSCs) group (HG-BMSCs group).
31138200	6	67	theme	control	829:835	arg1	group					837:841	control group	829:841	control group	829:841	They were assigned to different groups randomly (n = 6 per group): the osteochondral defect only group (control group), the osteochondral defect treated with hydrogel group (HG group), and the osteochondral defect treated with hydrogel loaded with bone marrow mesenchymal stem cells (BMSCs) group (HG-BMSCs group).
31138200	11	68	theme	PVA/CS	1497:1502	arg1	candidate					1546:1554	a good candidate	1539:1554	a good candidate for a tissue engineering material in cartilage repair	1539:1608	CONCLUSIONS The novel PVA/CS porous composite hydrogel could be a good candidate for a tissue engineering material in cartilage repair.
31138200	11	68	theme	PVA/CS	1497:1502	arg1	hydrogel					1521:1528	The novel PVA/CS porous composite hydrogel	1487:1528	The novel PVA/CS porous composite hydrogel	1487:1528	CONCLUSIONS The novel PVA/CS porous composite hydrogel could be a good candidate for a tissue engineering material in cartilage repair.
31138200	8	69	theme	chemical	1268:1275	arg1	properties					1277:1286	stable physical and chemical properties	1248:1286	stable physical and chemical properties	1248:1286	RESULTS The Hydrogel with PVA/CS ratio of 6:4 exhibited the best mechanical properties; it also showed stable physical and chemical properties with porosity and over 90% water content.
31138200	6	70	theme	cells	1002:1006	arg1	group					1016:1020	bone marrow mesenchymal stem cells (BMSCs) group	973:1020	bone marrow mesenchymal stem cells (BMSCs) group (HG-BMSCs group)	973:1037	They were assigned to different groups randomly (n = 6 per group): the osteochondral defect only group (control group), the osteochondral defect treated with hydrogel group (HG group), and the osteochondral defect treated with hydrogel loaded with bone marrow mesenchymal stem cells (BMSCs) group (HG-BMSCs group).
31138200	6	70	theme	cells	1002:1006	arg1	group					1032:1036	HG-BMSCs group	1023:1036	HG-BMSCs group	1023:1036	They were assigned to different groups randomly (n = 6 per group): the osteochondral defect only group (control group), the osteochondral defect treated with hydrogel group (HG group), and the osteochondral defect treated with hydrogel loaded with bone marrow mesenchymal stem cells (BMSCs) group (HG-BMSCs group).
31138200	6	71	theme	different	747:755	arg1	groups					757:762	different groups	747:762	different groups	747:762	They were assigned to different groups randomly (n = 6 per group): the osteochondral defect only group (control group), the osteochondral defect treated with hydrogel group (HG group), and the osteochondral defect treated with hydrogel loaded with bone marrow mesenchymal stem cells (BMSCs) group (HG-BMSCs group).
31138200	6	72	theme	stem	997:1000	arg1	BMSCs					1009:1013	BMSCs	1009:1013	BMSCs	1009:1013	They were assigned to different groups randomly (n = 6 per group): the osteochondral defect only group (control group), the osteochondral defect treated with hydrogel group (HG group), and the osteochondral defect treated with hydrogel loaded with bone marrow mesenchymal stem cells (BMSCs) group (HG-BMSCs group).
31138200	6	72	theme	stem	997:1000	arg1	cells					1002:1006	bone marrow mesenchymal stem cells	973:1006	bone marrow mesenchymal stem cells (BMSCs) group (HG-BMSCs group)	973:1037	They were assigned to different groups randomly (n = 6 per group): the osteochondral defect only group (control group), the osteochondral defect treated with hydrogel group (HG group), and the osteochondral defect treated with hydrogel loaded with bone marrow mesenchymal stem cells (BMSCs) group (HG-BMSCs group).
31138200	10	73	theme	cartilage	1456:1464	arg1	healing					1466:1472	the best cartilage healing	1447:1472	the best cartilage healing	1447:1472	The HG-BMSCs group achieved the best cartilage healing.
31138200	3	74	theme	ratios	462:467	arg1	Hydrogels					434:442	METHODS Hydrogels	426:442	METHODS Hydrogels of various PVA/CS ratios	426:467	METHODS Hydrogels of various PVA/CS ratios were tested for physiochemical and mechanical properties in addition to cytotoxicity and biocompatibility.
31138200	8	75	dep	RESULTS	1145:1151	arg1	Hydrogel					1157:1164	The Hydrogel	1153:1164	RESULTS The Hydrogel with PVA/CS ratio of 6:4	1145:1189	RESULTS The Hydrogel with PVA/CS ratio of 6:4 exhibited the best mechanical properties; it also showed stable physical and chemical properties with porosity and over 90% water content.
31138200	11	76	from	material	1581:1588	arg1	repair					1603:1608	cartilage repair	1593:1608	cartilage repair	1593:1608	CONCLUSIONS The novel PVA/CS porous composite hydrogel could be a good candidate for a tissue engineering material in cartilage repair.
31138200	0	77	from	application	128:138	arg1	repair					163:168	articular cartilage repair	143:168	articular cartilage repair	143:168	Characterization of a novel polyvinyl alcohol/chitosan porous hydrogel combined with bone marrow mesenchymal stem cells and its application in articular cartilage repair.
31138200	7	78	theme	histological	1094:1105	arg1	evaluation					1107:1116	macro-observation and histological evaluation	1072:1116	macro-observation and histological evaluation at 12 weeks after surgery	1072:1142	The cartilage was collected for macro-observation and histological evaluation at 12 weeks after surgery.
31138200	8	79	theme	best	1205:1208	arg1	properties					1221:1230	the best mechanical properties	1201:1230	the best mechanical properties	1201:1230	RESULTS The Hydrogel with PVA/CS ratio of 6:4 exhibited the best mechanical properties; it also showed stable physical and chemical properties with porosity and over 90% water content.
31138200	6	80	dep	randomly	764:771	arg1	n = 6					774:778	n = 6	774:778	n = 6 per group	774:788	They were assigned to different groups randomly (n = 6 per group): the osteochondral defect only group (control group), the osteochondral defect treated with hydrogel group (HG group), and the osteochondral defect treated with hydrogel loaded with bone marrow mesenchymal stem cells (BMSCs) group (HG-BMSCs group).
31138200	6	81	theme	marrow	978:983	arg1	BMSCs					1009:1013	BMSCs	1009:1013	BMSCs	1009:1013	They were assigned to different groups randomly (n = 6 per group): the osteochondral defect only group (control group), the osteochondral defect treated with hydrogel group (HG group), and the osteochondral defect treated with hydrogel loaded with bone marrow mesenchymal stem cells (BMSCs) group (HG-BMSCs group).
31138200	6	81	theme	marrow	978:983	arg1	cells					1002:1006	bone marrow mesenchymal stem cells	973:1006	bone marrow mesenchymal stem cells (BMSCs) group (HG-BMSCs group)	973:1037	They were assigned to different groups randomly (n = 6 per group): the osteochondral defect only group (control group), the osteochondral defect treated with hydrogel group (HG group), and the osteochondral defect treated with hydrogel loaded with bone marrow mesenchymal stem cells (BMSCs) group (HG-BMSCs group).
29458099	1	0	theme	radiata	312:318	arg1	wood					298:301	milled wood	291:301	milled wood of Pinus radiata	291:318	Near infrared (NIR) spectroscopy coupled with partial least squares (PLS-1) regression was used to predict the lignin contents and monosaccharide compositions of milled wood of Pinus radiata.
29458099	1	1	theme	NIR	144:146	arg1	spectroscopy					149:160	Near infrared (NIR) spectroscopy	129:160	Near infrared (NIR) spectroscopy coupled with partial least squares (PLS-1) regression	129:214	Near infrared (NIR) spectroscopy coupled with partial least squares (PLS-1) regression was used to predict the lignin contents and monosaccharide compositions of milled wood of Pinus radiata.
29458099	0	2	theme	monosaccharide	67:80	arg1	compositions					82:93	monosaccharide compositions	67:93	monosaccharide compositions	67:93	Using near infrared spectroscopy to predict the lignin content and monosaccharide compositions of Pinus radiata wood cell walls.
29458099	1	3	dep	contents	247:254	arg1	the					236:238	the	236:238	the	236:238	Near infrared (NIR) spectroscopy coupled with partial least squares (PLS-1) regression was used to predict the lignin contents and monosaccharide compositions of milled wood of Pinus radiata.
29458099	2	4	dep	types	430:434	arg1	<0.422mm					444:451	<0.422mm	444:451	<0.422mm	444:451	The effects of particle size and moisture content were investigated by collecting NIR spectra of four sample types: large (<0.422mm) and small (<0.178mm) particles, in both ambient and dry conditions.
29458099	2	4	dep	types	430:434	arg1	large					437:441	large	437:441	large	437:441	The effects of particle size and moisture content were investigated by collecting NIR spectra of four sample types: large (<0.422mm) and small (<0.178mm) particles, in both ambient and dry conditions.
29458099	2	4	dep	types	430:434	arg1	particles					475:483	small (<0.178mm) particles	458:483	small (<0.178mm) particles	458:483	The effects of particle size and moisture content were investigated by collecting NIR spectra of four sample types: large (<0.422mm) and small (<0.178mm) particles, in both ambient and dry conditions.
29458099	5	5	dep	particles	850:858	arg1	have					882:885	have	882:885	have the most efficient preparation and the standard error (SE) values for lignin (2.10%), arabinose (0.34%), xylose (1.33%), galactose (2.54%), glucose (6.98%), mannose (1.48%), galacturonic acid (0.22%), glucuronic acid (0.06%), and 4-O-methylglucuronic acid (0.25%)	882:1149	However, large particles in ambient conditions have the most efficient preparation and the standard error (SE) values for lignin (2.10%), arabinose (0.34%), xylose (1.33%), galactose (2.54%), glucose (6.98%), mannose (1.48%), galacturonic acid (0.22%), glucuronic acid (0.06%), and 4-O-methylglucuronic acid (0.25%) were achieved.
29458099	2	6	theme	size	345:348	arg1	effects					325:331	The effects	321:331	The effects of particle size and moisture content	321:369	The effects of particle size and moisture content were investigated by collecting NIR spectra of four sample types: large (<0.422mm) and small (<0.178mm) particles, in both ambient and dry conditions.
29458099	0	7	theme	Pinus	98:102	arg1	walls					122:126	Pinus radiata wood cell walls	98:126	Pinus radiata wood cell walls	98:126	Using near infrared spectroscopy to predict the lignin content and monosaccharide compositions of Pinus radiata wood cell walls.
29458099	5	8	theme	ambient	863:869	arg1	conditions					871:880	ambient conditions	863:880	ambient conditions	863:880	However, large particles in ambient conditions have the most efficient preparation and the standard error (SE) values for lignin (2.10%), arabinose (0.34%), xylose (1.33%), galactose (2.54%), glucose (6.98%), mannose (1.48%), galacturonic acid (0.22%), glucuronic acid (0.06%), and 4-O-methylglucuronic acid (0.25%) were achieved.
29458099	4	9	theme	NIR	797:799	arg1	spectra					801:807	NIR spectra	797:807	NIR spectra of all four sample types	797:832	Our results show that lignin contents and monosaccharide compositions of pure CWs and OWs can be successfully predicted using NIR spectra of all four sample types.
29458099	1	10	theme	lignin	240:245	arg1	contents					247:254	lignin contents	240:254	lignin contents	240:254	Near infrared (NIR) spectroscopy coupled with partial least squares (PLS-1) regression was used to predict the lignin contents and monosaccharide compositions of milled wood of Pinus radiata.
29458099	0	11	theme	wood	112:115	arg1	walls					122:126	Pinus radiata wood cell walls	98:126	Pinus radiata wood cell walls	98:126	Using near infrared spectroscopy to predict the lignin content and monosaccharide compositions of Pinus radiata wood cell walls.
29458099	2	12	theme	collecting	392:401	arg1	spectra					407:413	collecting NIR spectra	392:413	collecting NIR spectra of four sample types: large (<0.422mm) and small (<0.178mm) particles	392:483	The effects of particle size and moisture content were investigated by collecting NIR spectra of four sample types: large (<0.422mm) and small (<0.178mm) particles, in both ambient and dry conditions.
29458099	4	13	theme	lignin	693:698	arg1	contents					700:707	lignin contents	693:707	lignin contents	693:707	Our results show that lignin contents and monosaccharide compositions of pure CWs and OWs can be successfully predicted using NIR spectra of all four sample types.
29458099	4	14	theme	types	828:832	arg1	spectra					801:807	NIR spectra	797:807	NIR spectra of all four sample types	797:832	Our results show that lignin contents and monosaccharide compositions of pure CWs and OWs can be successfully predicted using NIR spectra of all four sample types.
29458099	0	15	theme	radiata	104:110	arg1	walls					122:126	Pinus radiata wood cell walls	98:126	Pinus radiata wood cell walls	98:126	Using near infrared spectroscopy to predict the lignin content and monosaccharide compositions of Pinus radiata wood cell walls.
29458099	4	16	theme	OWs	757:759	arg1	contents					700:707	lignin contents	693:707	lignin contents	693:707	Our results show that lignin contents and monosaccharide compositions of pure CWs and OWs can be successfully predicted using NIR spectra of all four sample types.
29458099	4	16	theme	OWs	757:759	arg1	compositions					728:739	monosaccharide compositions	713:739	monosaccharide compositions	713:739	Our results show that lignin contents and monosaccharide compositions of pure CWs and OWs can be successfully predicted using NIR spectra of all four sample types.
29458099	5	17	theme	glucuronic	1088:1097	arg1	%					1109:1109	0.06%	1105:1109	0.06%	1105:1109	However, large particles in ambient conditions have the most efficient preparation and the standard error (SE) values for lignin (2.10%), arabinose (0.34%), xylose (1.33%), galactose (2.54%), glucose (6.98%), mannose (1.48%), galacturonic acid (0.22%), glucuronic acid (0.06%), and 4-O-methylglucuronic acid (0.25%) were achieved.
29458099	5	17	theme	glucuronic	1088:1097	arg1	preparation					906:916	the most efficient preparation	887:916	the most efficient preparation	887:916	However, large particles in ambient conditions have the most efficient preparation and the standard error (SE) values for lignin (2.10%), arabinose (0.34%), xylose (1.33%), galactose (2.54%), glucose (6.98%), mannose (1.48%), galacturonic acid (0.22%), glucuronic acid (0.06%), and 4-O-methylglucuronic acid (0.25%) were achieved.
29458099	5	17	theme	glucuronic	1088:1097	arg1	acid					1099:1102	glucuronic acid	1088:1102	glucuronic acid (0.06%)	1088:1110	However, large particles in ambient conditions have the most efficient preparation and the standard error (SE) values for lignin (2.10%), arabinose (0.34%), xylose (1.33%), galactose (2.54%), glucose (6.98%), mannose (1.48%), galacturonic acid (0.22%), glucuronic acid (0.06%), and 4-O-methylglucuronic acid (0.25%) were achieved.
29458099	2	18	theme	<0.178mm	465:472	arg1	particles					475:483	small (<0.178mm) particles	458:483	small (<0.178mm) particles	458:483	The effects of particle size and moisture content were investigated by collecting NIR spectra of four sample types: large (<0.422mm) and small (<0.178mm) particles, in both ambient and dry conditions.
29458099	0	19	theme	near	6:9	arg1	spectroscopy					20:31	near infrared spectroscopy	6:31	near infrared spectroscopy	6:31	Using near infrared spectroscopy to predict the lignin content and monosaccharide compositions of Pinus radiata wood cell walls.
29458099	3	20	theme	linear	631:636	arg1	range					638:642	a linear range	629:642	a linear range of cell-wall compositions	629:668	PLS-1 models were constructed using mixtures of compression wood (CW) and opposite wood (OW) that provided a linear range of cell-wall compositions.
29458099	5	21	theme	large	844:848	arg1	particles					850:858	large particles	844:858	large particles in ambient conditions have the most efficient preparation and the standard error (SE) values for lignin (2.10%), arabinose (0.34%), xylose (1.33%), galactose (2.54%), glucose (6.98%), mannose (1.48%), galacturonic acid (0.22%), glucuronic acid (0.06%), and 4-O-methylglucuronic acid (0.25%)	844:1149	However, large particles in ambient conditions have the most efficient preparation and the standard error (SE) values for lignin (2.10%), arabinose (0.34%), xylose (1.33%), galactose (2.54%), glucose (6.98%), mannose (1.48%), galacturonic acid (0.22%), glucuronic acid (0.06%), and 4-O-methylglucuronic acid (0.25%) were achieved.
29458099	2	22	theme	moisture	354:361	arg1	content					363:369	moisture content	354:369	moisture content	354:369	The effects of particle size and moisture content were investigated by collecting NIR spectra of four sample types: large (<0.422mm) and small (<0.178mm) particles, in both ambient and dry conditions.
29458099	3	23	theme	wood	605:608	arg1	mixtures					558:565	mixtures	558:565	mixtures of compression wood (CW) and opposite wood (OW) that provided a linear range of cell-wall compositions	558:668	PLS-1 models were constructed using mixtures of compression wood (CW) and opposite wood (OW) that provided a linear range of cell-wall compositions.
29458099	1	24	theme	partial	175:181	arg1	squares					189:195	partial least squares	175:195	partial least squares (PLS-1) regression	175:214	Near infrared (NIR) spectroscopy coupled with partial least squares (PLS-1) regression was used to predict the lignin contents and monosaccharide compositions of milled wood of Pinus radiata.
29458099	1	24	theme	partial	175:181	arg1	PLS-1					198:202	PLS-1	198:202	PLS-1	198:202	Near infrared (NIR) spectroscopy coupled with partial least squares (PLS-1) regression was used to predict the lignin contents and monosaccharide compositions of milled wood of Pinus radiata.
29458099	0	25	theme	walls	122:126	arg1	content					55:61	lignin content	48:61	lignin content	48:61	Using near infrared spectroscopy to predict the lignin content and monosaccharide compositions of Pinus radiata wood cell walls.
29458099	0	25	theme	walls	122:126	arg1	compositions					82:93	monosaccharide compositions	67:93	monosaccharide compositions	67:93	Using near infrared spectroscopy to predict the lignin content and monosaccharide compositions of Pinus radiata wood cell walls.
29458099	5	26	theme	standard	926:933	arg1	error					935:939	the standard error	922:939	the standard error (SE) values for lignin (2.10%)	922:970	However, large particles in ambient conditions have the most efficient preparation and the standard error (SE) values for lignin (2.10%), arabinose (0.34%), xylose (1.33%), galactose (2.54%), glucose (6.98%), mannose (1.48%), galacturonic acid (0.22%), glucuronic acid (0.06%), and 4-O-methylglucuronic acid (0.25%) were achieved.
29458099	5	26	theme	standard	926:933	arg1	SE					942:943	SE	942:943	SE	942:943	However, large particles in ambient conditions have the most efficient preparation and the standard error (SE) values for lignin (2.10%), arabinose (0.34%), xylose (1.33%), galactose (2.54%), glucose (6.98%), mannose (1.48%), galacturonic acid (0.22%), glucuronic acid (0.06%), and 4-O-methylglucuronic acid (0.25%) were achieved.
29458099	1	27	theme	least	183:187	arg1	squares					189:195	partial least squares	175:195	partial least squares (PLS-1) regression	175:214	Near infrared (NIR) spectroscopy coupled with partial least squares (PLS-1) regression was used to predict the lignin contents and monosaccharide compositions of milled wood of Pinus radiata.
29458099	1	27	theme	least	183:187	arg1	PLS-1					198:202	PLS-1	198:202	PLS-1	198:202	Near infrared (NIR) spectroscopy coupled with partial least squares (PLS-1) regression was used to predict the lignin contents and monosaccharide compositions of milled wood of Pinus radiata.
29458099	0	28	theme	infrared	11:18	arg1	spectroscopy					20:31	near infrared spectroscopy	6:31	near infrared spectroscopy	6:31	Using near infrared spectroscopy to predict the lignin content and monosaccharide compositions of Pinus radiata wood cell walls.
29458099	1	29	theme	monosaccharide	260:273	arg1	compositions					275:286	monosaccharide compositions	260:286	monosaccharide compositions	260:286	Near infrared (NIR) spectroscopy coupled with partial least squares (PLS-1) regression was used to predict the lignin contents and monosaccharide compositions of milled wood of Pinus radiata.
29458099	0	30	theme	cell	117:120	arg1	walls					122:126	Pinus radiata wood cell walls	98:126	Pinus radiata wood cell walls	98:126	Using near infrared spectroscopy to predict the lignin content and monosaccharide compositions of Pinus radiata wood cell walls.
29458099	0	31	dep	content	55:61	arg1	the					44:46	the	44:46	the	44:46	Using near infrared spectroscopy to predict the lignin content and monosaccharide compositions of Pinus radiata wood cell walls.
29458099	1	32	theme	squares	189:195	arg1	regression					205:214	partial least squares (PLS-1) regression	175:214	partial least squares (PLS-1) regression	175:214	Near infrared (NIR) spectroscopy coupled with partial least squares (PLS-1) regression was used to predict the lignin contents and monosaccharide compositions of milled wood of Pinus radiata.
29458099	3	33	theme	compositions	657:668	arg1	range					638:642	a linear range	629:642	a linear range of cell-wall compositions	629:668	PLS-1 models were constructed using mixtures of compression wood (CW) and opposite wood (OW) that provided a linear range of cell-wall compositions.
29458099	4	34	theme	pure	744:747	arg1	CWs					749:751	pure CWs	744:751	pure CWs	744:751	Our results show that lignin contents and monosaccharide compositions of pure CWs and OWs can be successfully predicted using NIR spectra of all four sample types.
29458099	5	35	theme	galacturonic	1061:1072	arg1	acid					1074:1077	galacturonic acid	1061:1077	galacturonic acid (0.22%)	1061:1085	However, large particles in ambient conditions have the most efficient preparation and the standard error (SE) values for lignin (2.10%), arabinose (0.34%), xylose (1.33%), galactose (2.54%), glucose (6.98%), mannose (1.48%), galacturonic acid (0.22%), glucuronic acid (0.06%), and 4-O-methylglucuronic acid (0.25%) were achieved.
29458099	5	35	theme	galacturonic	1061:1072	arg1	%					1084:1084	0.22%	1080:1084	0.22%	1080:1084	However, large particles in ambient conditions have the most efficient preparation and the standard error (SE) values for lignin (2.10%), arabinose (0.34%), xylose (1.33%), galactose (2.54%), glucose (6.98%), mannose (1.48%), galacturonic acid (0.22%), glucuronic acid (0.06%), and 4-O-methylglucuronic acid (0.25%) were achieved.
29458099	5	35	theme	galacturonic	1061:1072	arg1	preparation					906:916	the most efficient preparation	887:916	the most efficient preparation	887:916	However, large particles in ambient conditions have the most efficient preparation and the standard error (SE) values for lignin (2.10%), arabinose (0.34%), xylose (1.33%), galactose (2.54%), glucose (6.98%), mannose (1.48%), galacturonic acid (0.22%), glucuronic acid (0.06%), and 4-O-methylglucuronic acid (0.25%) were achieved.
29458099	3	36	theme	wood	582:585	arg1	mixtures					558:565	mixtures	558:565	mixtures of compression wood (CW) and opposite wood (OW) that provided a linear range of cell-wall compositions	558:668	PLS-1 models were constructed using mixtures of compression wood (CW) and opposite wood (OW) that provided a linear range of cell-wall compositions.
29458099	5	37	theme	error	935:939	arg1	values					946:951	the standard error (SE) values	922:951	the standard error (SE) values for lignin (2.10%)	922:970	However, large particles in ambient conditions have the most efficient preparation and the standard error (SE) values for lignin (2.10%), arabinose (0.34%), xylose (1.33%), galactose (2.54%), glucose (6.98%), mannose (1.48%), galacturonic acid (0.22%), glucuronic acid (0.06%), and 4-O-methylglucuronic acid (0.25%) were achieved.
29458099	5	37	theme	error	935:939	arg1	arabinose					973:981	arabinose	973:981	arabinose	973:981	However, large particles in ambient conditions have the most efficient preparation and the standard error (SE) values for lignin (2.10%), arabinose (0.34%), xylose (1.33%), galactose (2.54%), glucose (6.98%), mannose (1.48%), galacturonic acid (0.22%), glucuronic acid (0.06%), and 4-O-methylglucuronic acid (0.25%) were achieved.
29458099	0	38	theme	lignin	48:53	arg1	content					55:61	lignin content	48:61	lignin content	48:61	Using near infrared spectroscopy to predict the lignin content and monosaccharide compositions of Pinus radiata wood cell walls.
29458099	1	39	theme	milled	291:296	arg1	wood					298:301	milled wood	291:301	milled wood of Pinus radiata	291:318	Near infrared (NIR) spectroscopy coupled with partial least squares (PLS-1) regression was used to predict the lignin contents and monosaccharide compositions of milled wood of Pinus radiata.
29458099	3	40	theme	PLS-1	522:526	arg1	models					528:533	PLS-1 models	522:533	PLS-1 models	522:533	PLS-1 models were constructed using mixtures of compression wood (CW) and opposite wood (OW) that provided a linear range of cell-wall compositions.
29458099	4	41	theme	sample	821:826	arg1	types					828:832	all four sample types	812:832	all four sample types	812:832	Our results show that lignin contents and monosaccharide compositions of pure CWs and OWs can be successfully predicted using NIR spectra of all four sample types.
29458099	2	42	theme	particle	336:343	arg1	size					345:348	particle size	336:348	particle size	336:348	The effects of particle size and moisture content were investigated by collecting NIR spectra of four sample types: large (<0.422mm) and small (<0.178mm) particles, in both ambient and dry conditions.
29458099	1	43	theme	wood	298:301	arg1	contents					247:254	lignin contents	240:254	lignin contents	240:254	Near infrared (NIR) spectroscopy coupled with partial least squares (PLS-1) regression was used to predict the lignin contents and monosaccharide compositions of milled wood of Pinus radiata.
29458099	1	43	theme	wood	298:301	arg1	compositions					275:286	monosaccharide compositions	260:286	monosaccharide compositions	260:286	Near infrared (NIR) spectroscopy coupled with partial least squares (PLS-1) regression was used to predict the lignin contents and monosaccharide compositions of milled wood of Pinus radiata.
29458099	2	44	theme	ambient	494:500	arg1	conditions					510:519	both ambient and dry conditions	489:519	conditions	510:519	The effects of particle size and moisture content were investigated by collecting NIR spectra of four sample types: large (<0.422mm) and small (<0.178mm) particles, in both ambient and dry conditions.
29458099	1	45	used	used	220:223	arg2	spectroscopy					149:160	Near infrared (NIR) spectroscopy	129:160	Near infrared (NIR) spectroscopy coupled with partial least squares (PLS-1) regression	129:214	Near infrared (NIR) spectroscopy coupled with partial least squares (PLS-1) regression was used to predict the lignin contents and monosaccharide compositions of milled wood of Pinus radiata.
29458099	4	46	theme	CWs	749:751	arg1	contents					700:707	lignin contents	693:707	lignin contents	693:707	Our results show that lignin contents and monosaccharide compositions of pure CWs and OWs can be successfully predicted using NIR spectra of all four sample types.
29458099	4	46	theme	CWs	749:751	arg1	compositions					728:739	monosaccharide compositions	713:739	monosaccharide compositions	713:739	Our results show that lignin contents and monosaccharide compositions of pure CWs and OWs can be successfully predicted using NIR spectra of all four sample types.
29458099	5	47	theme	4-O-methylglucuronic	1117:1136	arg1	preparation					906:916	the most efficient preparation	887:916	the most efficient preparation	887:916	However, large particles in ambient conditions have the most efficient preparation and the standard error (SE) values for lignin (2.10%), arabinose (0.34%), xylose (1.33%), galactose (2.54%), glucose (6.98%), mannose (1.48%), galacturonic acid (0.22%), glucuronic acid (0.06%), and 4-O-methylglucuronic acid (0.25%) were achieved.
29458099	5	47	theme	4-O-methylglucuronic	1117:1136	arg1	acid					1138:1141	4-O-methylglucuronic acid	1117:1141	4-O-methylglucuronic acid	1117:1141	However, large particles in ambient conditions have the most efficient preparation and the standard error (SE) values for lignin (2.10%), arabinose (0.34%), xylose (1.33%), galactose (2.54%), glucose (6.98%), mannose (1.48%), galacturonic acid (0.22%), glucuronic acid (0.06%), and 4-O-methylglucuronic acid (0.25%) were achieved.
29458099	2	48	theme	dry	506:508	arg1	conditions					510:519	both ambient and dry conditions	489:519	conditions	510:519	The effects of particle size and moisture content were investigated by collecting NIR spectra of four sample types: large (<0.422mm) and small (<0.178mm) particles, in both ambient and dry conditions.
29458099	5	49	theme	efficient	896:904	arg1	glucose					1027:1033	glucose	1027:1033	glucose (6.98%)	1027:1041	However, large particles in ambient conditions have the most efficient preparation and the standard error (SE) values for lignin (2.10%), arabinose (0.34%), xylose (1.33%), galactose (2.54%), glucose (6.98%), mannose (1.48%), galacturonic acid (0.22%), glucuronic acid (0.06%), and 4-O-methylglucuronic acid (0.25%) were achieved.
29458099	5	49	theme	efficient	896:904	arg1	mannose					1044:1050	mannose	1044:1050	mannose (1.48%)	1044:1058	However, large particles in ambient conditions have the most efficient preparation and the standard error (SE) values for lignin (2.10%), arabinose (0.34%), xylose (1.33%), galactose (2.54%), glucose (6.98%), mannose (1.48%), galacturonic acid (0.22%), glucuronic acid (0.06%), and 4-O-methylglucuronic acid (0.25%) were achieved.
29458099	5	49	theme	efficient	896:904	arg1	acid					1099:1102	glucuronic acid	1088:1102	glucuronic acid (0.06%)	1088:1110	However, large particles in ambient conditions have the most efficient preparation and the standard error (SE) values for lignin (2.10%), arabinose (0.34%), xylose (1.33%), galactose (2.54%), glucose (6.98%), mannose (1.48%), galacturonic acid (0.22%), glucuronic acid (0.06%), and 4-O-methylglucuronic acid (0.25%) were achieved.
29458099	5	49	theme	efficient	896:904	arg1	galactose					1008:1016	galactose	1008:1016	galactose (2.54%)	1008:1024	However, large particles in ambient conditions have the most efficient preparation and the standard error (SE) values for lignin (2.10%), arabinose (0.34%), xylose (1.33%), galactose (2.54%), glucose (6.98%), mannose (1.48%), galacturonic acid (0.22%), glucuronic acid (0.06%), and 4-O-methylglucuronic acid (0.25%) were achieved.
29458099	5	49	theme	efficient	896:904	arg1	acid					1074:1077	galacturonic acid	1061:1077	galacturonic acid (0.22%)	1061:1085	However, large particles in ambient conditions have the most efficient preparation and the standard error (SE) values for lignin (2.10%), arabinose (0.34%), xylose (1.33%), galactose (2.54%), glucose (6.98%), mannose (1.48%), galacturonic acid (0.22%), glucuronic acid (0.06%), and 4-O-methylglucuronic acid (0.25%) were achieved.
29458099	5	49	theme	efficient	896:904	arg1	xylose					992:997	xylose	992:997	xylose (1.33%)	992:1005	However, large particles in ambient conditions have the most efficient preparation and the standard error (SE) values for lignin (2.10%), arabinose (0.34%), xylose (1.33%), galactose (2.54%), glucose (6.98%), mannose (1.48%), galacturonic acid (0.22%), glucuronic acid (0.06%), and 4-O-methylglucuronic acid (0.25%) were achieved.
29458099	5	49	theme	efficient	896:904	arg1	acid					1138:1141	4-O-methylglucuronic acid	1117:1141	4-O-methylglucuronic acid	1117:1141	However, large particles in ambient conditions have the most efficient preparation and the standard error (SE) values for lignin (2.10%), arabinose (0.34%), xylose (1.33%), galactose (2.54%), glucose (6.98%), mannose (1.48%), galacturonic acid (0.22%), glucuronic acid (0.06%), and 4-O-methylglucuronic acid (0.25%) were achieved.
29458099	5	49	theme	efficient	896:904	arg1	preparation					906:916	the most efficient preparation	887:916	the most efficient preparation	887:916	However, large particles in ambient conditions have the most efficient preparation and the standard error (SE) values for lignin (2.10%), arabinose (0.34%), xylose (1.33%), galactose (2.54%), glucose (6.98%), mannose (1.48%), galacturonic acid (0.22%), glucuronic acid (0.06%), and 4-O-methylglucuronic acid (0.25%) were achieved.
29458099	5	49	theme	efficient	896:904	arg1	%					988:988	0.34%	984:988	0.34%	984:988	However, large particles in ambient conditions have the most efficient preparation and the standard error (SE) values for lignin (2.10%), arabinose (0.34%), xylose (1.33%), galactose (2.54%), glucose (6.98%), mannose (1.48%), galacturonic acid (0.22%), glucuronic acid (0.06%), and 4-O-methylglucuronic acid (0.25%) were achieved.
29458099	3	50	theme	cell-wall	647:655	arg1	compositions					657:668	cell-wall compositions	647:668	cell-wall compositions	647:668	PLS-1 models were constructed using mixtures of compression wood (CW) and opposite wood (OW) that provided a linear range of cell-wall compositions.
29458099	5	51	from	particles	850:858	arg1	conditions					871:880	ambient conditions	863:880	ambient conditions	863:880	However, large particles in ambient conditions have the most efficient preparation and the standard error (SE) values for lignin (2.10%), arabinose (0.34%), xylose (1.33%), galactose (2.54%), glucose (6.98%), mannose (1.48%), galacturonic acid (0.22%), glucuronic acid (0.06%), and 4-O-methylglucuronic acid (0.25%) were achieved.
29458099	3	52	theme	compression	570:580	arg1	CW					588:589	CW	588:589	CW	588:589	PLS-1 models were constructed using mixtures of compression wood (CW) and opposite wood (OW) that provided a linear range of cell-wall compositions.
29458099	3	52	theme	compression	570:580	arg1	wood					582:585	compression wood	570:585	compression wood (CW)	570:590	PLS-1 models were constructed using mixtures of compression wood (CW) and opposite wood (OW) that provided a linear range of cell-wall compositions.
29458099	1	53	theme	Near	129:132	arg1	spectroscopy					149:160	Near infrared (NIR) spectroscopy	129:160	Near infrared (NIR) spectroscopy coupled with partial least squares (PLS-1) regression	129:214	Near infrared (NIR) spectroscopy coupled with partial least squares (PLS-1) regression was used to predict the lignin contents and monosaccharide compositions of milled wood of Pinus radiata.
29458099	2	54	theme	types	430:434	arg1	spectra					407:413	collecting NIR spectra	392:413	collecting NIR spectra of four sample types: large (<0.422mm) and small (<0.178mm) particles	392:483	The effects of particle size and moisture content were investigated by collecting NIR spectra of four sample types: large (<0.422mm) and small (<0.178mm) particles, in both ambient and dry conditions.
29458099	5	55	theme	6.98	1036:1039	arg1	glucose					1027:1033	glucose	1027:1033	glucose (6.98%)	1027:1041	However, large particles in ambient conditions have the most efficient preparation and the standard error (SE) values for lignin (2.10%), arabinose (0.34%), xylose (1.33%), galactose (2.54%), glucose (6.98%), mannose (1.48%), galacturonic acid (0.22%), glucuronic acid (0.06%), and 4-O-methylglucuronic acid (0.25%) were achieved.
29458099	5	55	theme	6.98	1036:1039	arg1	%					1040:1040	6.98%	1036:1040	6.98%	1036:1040	However, large particles in ambient conditions have the most efficient preparation and the standard error (SE) values for lignin (2.10%), arabinose (0.34%), xylose (1.33%), galactose (2.54%), glucose (6.98%), mannose (1.48%), galacturonic acid (0.22%), glucuronic acid (0.06%), and 4-O-methylglucuronic acid (0.25%) were achieved.
29458099	2	56	theme	small	458:462	arg1	particles					475:483	small (<0.178mm) particles	458:483	small (<0.178mm) particles	458:483	The effects of particle size and moisture content were investigated by collecting NIR spectra of four sample types: large (<0.422mm) and small (<0.178mm) particles, in both ambient and dry conditions.
29458099	3	57	theme	opposite	596:603	arg1	OW					611:612	OW	611:612	OW	611:612	PLS-1 models were constructed using mixtures of compression wood (CW) and opposite wood (OW) that provided a linear range of cell-wall compositions.
29458099	3	57	theme	opposite	596:603	arg1	wood					605:608	opposite wood	596:608	opposite wood (OW)	596:613	PLS-1 models were constructed using mixtures of compression wood (CW) and opposite wood (OW) that provided a linear range of cell-wall compositions.
29458099	2	58	theme	NIR	403:405	arg1	spectra					407:413	collecting NIR spectra	392:413	collecting NIR spectra of four sample types: large (<0.422mm) and small (<0.178mm) particles	392:483	The effects of particle size and moisture content were investigated by collecting NIR spectra of four sample types: large (<0.422mm) and small (<0.178mm) particles, in both ambient and dry conditions.
29458099	1	59	theme	infrared	134:141	arg1	spectroscopy					149:160	Near infrared (NIR) spectroscopy	129:160	Near infrared (NIR) spectroscopy coupled with partial least squares (PLS-1) regression	129:214	Near infrared (NIR) spectroscopy coupled with partial least squares (PLS-1) regression was used to predict the lignin contents and monosaccharide compositions of milled wood of Pinus radiata.
29458099	2	60	theme	content	363:369	arg1	effects					325:331	The effects	321:331	The effects of particle size and moisture content	321:369	The effects of particle size and moisture content were investigated by collecting NIR spectra of four sample types: large (<0.422mm) and small (<0.178mm) particles, in both ambient and dry conditions.
29458099	1	61	theme	Pinus	306:310	arg1	radiata					312:318	Pinus radiata	306:318	Pinus radiata	306:318	Near infrared (NIR) spectroscopy coupled with partial least squares (PLS-1) regression was used to predict the lignin contents and monosaccharide compositions of milled wood of Pinus radiata.
29458099	4	62	theme	monosaccharide	713:726	arg1	compositions					728:739	monosaccharide compositions	713:739	monosaccharide compositions	713:739	Our results show that lignin contents and monosaccharide compositions of pure CWs and OWs can be successfully predicted using NIR spectra of all four sample types.
29458099	2	63	theme	sample	423:428	arg1	types					430:434	four sample types	418:434	four sample types: large (<0.422mm) and small (<0.178mm) particles	418:483	The effects of particle size and moisture content were investigated by collecting NIR spectra of four sample types: large (<0.422mm) and small (<0.178mm) particles, in both ambient and dry conditions.
29682888	8	0	theme	conventional	1174:1185	arg1	vaccination					1187:1197	conventional vaccination	1174:1197	conventional vaccination with BCG	1174:1206	However, they did not improve protective efficacy in mice against challenge with M. tuberculosis compared to conventional vaccination with BCG, suggesting that additional or alternative antigens may be needed to protect against this disease.
29682888	6	1	theme	increased	920:928	arg1	affinity					930:937	increased affinity	920:937	increased affinity for low-affinity FcγRs	920:960	Modification of the N-glycans linked to TB-PIGS by removal of xylose and fucose residues that are normally found in plant glycosylated proteins also resulted in increased affinity for low-affinity FcγRs.
29682888	3	2	theme	Nicotiana	500:508	arg1	benthamiana					510:520	Nicotiana benthamiana	500:520	Nicotiana benthamiana	500:520	Expression was attempted in Chinese Hamster Ovary (CHO) cells and in Nicotiana benthamiana.
29682888	8	3	with	challenge	1131:1139	arg1	M. tuberculosis					1146:1160	M. tuberculosis	1146:1160	M. tuberculosis	1146:1160	However, they did not improve protective efficacy in mice against challenge with M. tuberculosis compared to conventional vaccination with BCG, suggesting that additional or alternative antigens may be needed to protect against this disease.
29682888	4	4	from	similar	612:618	arg1	size					623:626	size	623:626	size	623:626	The recombinant protein assembled into polymeric structures (TB-PIGS) in N. benthamiana, similar in size to polymeric IgM.
29682888	6	5	theme	xylose	821:826	arg1	residues					839:846	xylose and fucose residues	821:846	xylose and fucose residues that are normally found in plant glycosylated proteins	821:901	Modification of the N-glycans linked to TB-PIGS by removal of xylose and fucose residues that are normally found in plant glycosylated proteins also resulted in increased affinity for low-affinity FcγRs.
29682888	9	6	theme	functional	1430:1439	arg1	characteristics					1441:1455	inherent functional characteristics	1421:1455	inherent functional characteristics that are desirable in vaccines	1421:1486	Nevertheless, these results establish a novel platform for producing polymeric antigen-IgG γ-chain molecules with inherent functional characteristics that are desirable in vaccines.
29682888	7	7	from	studies	976:982	arg1	mice					987:990	mice	987:990	mice	987:990	Immunization studies in mice indicated that TB-PIGS are highly immunogenic with and without adjuvant.
29682888	7	8	theme	Immunization	963:974	arg1	studies					976:982	Immunization studies	963:982	Immunization studies in mice	963:990	Immunization studies in mice indicated that TB-PIGS are highly immunogenic with and without adjuvant.
29682888	5	9	theme	increased	739:747	arg1	affinity					749:756	increased affinity	739:756	increased affinity	739:756	These complexes were subsequently shown to bind to the complement protein C1q and FcγRs with increased affinity.
29682888	9	10	theme	novel	1347:1351	arg1	platform					1353:1360	a novel platform	1345:1360	a novel platform for producing polymeric antigen-IgG γ-chain molecules with inherent functional characteristics that are desirable in vaccines	1345:1486	Nevertheless, these results establish a novel platform for producing polymeric antigen-IgG γ-chain molecules with inherent functional characteristics that are desirable in vaccines.
29682888	4	11	theme	polymeric	631:639	arg1	IgM					641:643	polymeric IgM	631:643	polymeric IgM	631:643	The recombinant protein assembled into polymeric structures (TB-PIGS) in N. benthamiana, similar in size to polymeric IgM.
29682888	6	12	theme	glycosylated	881:892	arg1	proteins					894:901	plant glycosylated proteins	875:901	plant glycosylated proteins	875:901	Modification of the N-glycans linked to TB-PIGS by removal of xylose and fucose residues that are normally found in plant glycosylated proteins also resulted in increased affinity for low-affinity FcγRs.
29682888	2	13	theme	μ	407:407	arg1	chain					409:413	immunoglobulin μ chain	392:413	immunoglobulin μ chain	392:413	A genetic fusion construct comprising genes encoding the mycobacterial Ag85B antigen, an immunoglobulin γ-chain fragment and the tailpiece from immunoglobulin μ chain was engineered.
29682888	0	14	theme	polymeric	2:10	arg1	strategy					50:57	A polymeric immunoglobulin-antigen fusion protein strategy	0:57	A polymeric immunoglobulin-antigen fusion protein strategy for enhancing vaccine immunogenicity.	0:95	A polymeric immunoglobulin-antigen fusion protein strategy for enhancing vaccine immunogenicity.
29682888	6	15	attach	linked	789:794	arg2	N-glycans					779:787	the N-glycans	775:787	the N-glycans linked to TB-PIGS	775:805	Modification of the N-glycans linked to TB-PIGS by removal of xylose and fucose residues that are normally found in plant glycosylated proteins also resulted in increased affinity for low-affinity FcγRs.
29682888	6	15	attach	linked	789:794	arg1	TB-PIGS					799:805	TB-PIGS	799:805	TB-PIGS	799:805	Modification of the N-glycans linked to TB-PIGS by removal of xylose and fucose residues that are normally found in plant glycosylated proteins also resulted in increased affinity for low-affinity FcγRs.
29682888	9	16	from	desirable	1466:1474	arg1	vaccines					1479:1486	vaccines	1479:1486	vaccines	1479:1486	Nevertheless, these results establish a novel platform for producing polymeric antigen-IgG γ-chain molecules with inherent functional characteristics that are desirable in vaccines.
29682888	2	17	theme	immunoglobulin	392:405	arg1	chain					409:413	immunoglobulin μ chain	392:413	immunoglobulin μ chain	392:413	A genetic fusion construct comprising genes encoding the mycobacterial Ag85B antigen, an immunoglobulin γ-chain fragment and the tailpiece from immunoglobulin μ chain was engineered.
29682888	9	18	theme	polymeric	1376:1384	arg1	molecules					1406:1414	polymeric antigen-IgG γ-chain molecules	1376:1414	polymeric antigen-IgG γ-chain molecules	1376:1414	Nevertheless, these results establish a novel platform for producing polymeric antigen-IgG γ-chain molecules with inherent functional characteristics that are desirable in vaccines.
29682888	9	19	theme	inherent	1421:1428	arg1	characteristics					1441:1455	inherent functional characteristics	1421:1455	inherent functional characteristics that are desirable in vaccines	1421:1486	Nevertheless, these results establish a novel platform for producing polymeric antigen-IgG γ-chain molecules with inherent functional characteristics that are desirable in vaccines.
29682888	2	20	theme	Ag85B	319:323	arg1	fragment					360:367	an immunoglobulin γ-chain fragment	334:367	an immunoglobulin γ-chain fragment	334:367	A genetic fusion construct comprising genes encoding the mycobacterial Ag85B antigen, an immunoglobulin γ-chain fragment and the tailpiece from immunoglobulin μ chain was engineered.
29682888	2	20	theme	Ag85B	319:323	arg1	antigen					325:331	the mycobacterial Ag85B antigen	301:331	the mycobacterial Ag85B antigen	301:331	A genetic fusion construct comprising genes encoding the mycobacterial Ag85B antigen, an immunoglobulin γ-chain fragment and the tailpiece from immunoglobulin μ chain was engineered.
29682888	0	21	theme	fusion	35:40	arg1	strategy					50:57	A polymeric immunoglobulin-antigen fusion protein strategy	0:57	A polymeric immunoglobulin-antigen fusion protein strategy for enhancing vaccine immunogenicity.	0:95	A polymeric immunoglobulin-antigen fusion protein strategy for enhancing vaccine immunogenicity.
29682888	4	22	theme	polymeric	562:570	arg1	structures					572:581	polymeric structures	562:581	polymeric structures (TB-PIGS) in N. benthamiana, similar in size to polymeric IgM	562:643	The recombinant protein assembled into polymeric structures (TB-PIGS) in N. benthamiana, similar in size to polymeric IgM.
29682888	4	22	theme	polymeric	562:570	arg1	TB-PIGS					584:590	TB-PIGS	584:590	TB-PIGS	584:590	The recombinant protein assembled into polymeric structures (TB-PIGS) in N. benthamiana, similar in size to polymeric IgM.
29682888	5	23	theme	complement	701:710	arg1	C1q					720:722	the complement protein C1q	697:722	the complement protein C1q	697:722	These complexes were subsequently shown to bind to the complement protein C1q and FcγRs with increased affinity.
29682888	6	24	theme	N-glycans	779:787	arg1	Modification					759:770	Modification	759:770	Modification of the N-glycans linked to TB-PIGS by removal of xylose and fucose residues that are normally found in plant glycosylated proteins	759:901	Modification of the N-glycans linked to TB-PIGS by removal of xylose and fucose residues that are normally found in plant glycosylated proteins also resulted in increased affinity for low-affinity FcγRs.
29682888	2	25	theme	mycobacterial	305:317	arg1	fragment					360:367	an immunoglobulin γ-chain fragment	334:367	an immunoglobulin γ-chain fragment	334:367	A genetic fusion construct comprising genes encoding the mycobacterial Ag85B antigen, an immunoglobulin γ-chain fragment and the tailpiece from immunoglobulin μ chain was engineered.
29682888	2	25	theme	mycobacterial	305:317	arg1	antigen					325:331	the mycobacterial Ag85B antigen	301:331	the mycobacterial Ag85B antigen	301:331	A genetic fusion construct comprising genes encoding the mycobacterial Ag85B antigen, an immunoglobulin γ-chain fragment and the tailpiece from immunoglobulin μ chain was engineered.
29682888	6	26	located	found	866:870	arg1	proteins					894:901	plant glycosylated proteins	875:901	plant glycosylated proteins	875:901	Modification of the N-glycans linked to TB-PIGS by removal of xylose and fucose residues that are normally found in plant glycosylated proteins also resulted in increased affinity for low-affinity FcγRs.
29682888	6	26	located	found	866:870	arg2	residues					839:846	xylose and fucose residues	821:846	xylose and fucose residues that are normally found in plant glycosylated proteins	821:901	Modification of the N-glycans linked to TB-PIGS by removal of xylose and fucose residues that are normally found in plant glycosylated proteins also resulted in increased affinity for low-affinity FcγRs.
29682888	5	27	with	C1q	720:722	arg1	affinity					749:756	increased affinity	739:756	increased affinity	739:756	These complexes were subsequently shown to bind to the complement protein C1q and FcγRs with increased affinity.
29682888	0	28	theme	immunoglobulin-antigen	12:33	arg1	strategy					50:57	A polymeric immunoglobulin-antigen fusion protein strategy	0:57	A polymeric immunoglobulin-antigen fusion protein strategy for enhancing vaccine immunogenicity.	0:95	A polymeric immunoglobulin-antigen fusion protein strategy for enhancing vaccine immunogenicity.
29682888	9	29	theme	antigen-IgG	1386:1396	arg1	molecules					1406:1414	polymeric antigen-IgG γ-chain molecules	1376:1414	polymeric antigen-IgG γ-chain molecules	1376:1414	Nevertheless, these results establish a novel platform for producing polymeric antigen-IgG γ-chain molecules with inherent functional characteristics that are desirable in vaccines.
29682888	5	30	theme	protein	712:718	arg1	C1q					720:722	the complement protein C1q	697:722	the complement protein C1q	697:722	These complexes were subsequently shown to bind to the complement protein C1q and FcγRs with increased affinity.
29682888	3	31	theme	CHO	482:484	arg1	cells					487:491	Chinese Hamster Ovary (CHO) cells	459:491	Chinese Hamster Ovary (CHO) cells	459:491	Expression was attempted in Chinese Hamster Ovary (CHO) cells and in Nicotiana benthamiana.
29682888	9	32	theme	γ-chain	1398:1404	arg1	molecules					1406:1414	polymeric antigen-IgG γ-chain molecules	1376:1414	polymeric antigen-IgG γ-chain molecules	1376:1414	Nevertheless, these results establish a novel platform for producing polymeric antigen-IgG γ-chain molecules with inherent functional characteristics that are desirable in vaccines.
29682888	6	33	gly	glycosylated	881:892	arg1	proteins					894:901	plant glycosylated proteins	875:901	plant glycosylated proteins	875:901	Modification of the N-glycans linked to TB-PIGS by removal of xylose and fucose residues that are normally found in plant glycosylated proteins also resulted in increased affinity for low-affinity FcγRs.
29682888	3	34	theme	Hamster	467:473	arg1	cells					487:491	Chinese Hamster Ovary (CHO) cells	459:491	Chinese Hamster Ovary (CHO) cells	459:491	Expression was attempted in Chinese Hamster Ovary (CHO) cells and in Nicotiana benthamiana.
29682888	0	35	theme	protein	42:48	arg1	strategy					50:57	A polymeric immunoglobulin-antigen fusion protein strategy	0:57	A polymeric immunoglobulin-antigen fusion protein strategy for enhancing vaccine immunogenicity.	0:95	A polymeric immunoglobulin-antigen fusion protein strategy for enhancing vaccine immunogenicity.
29682888	8	36	theme	alternative	1239:1249	arg1	antigens					1251:1258	additional or alternative antigens	1225:1258	additional or alternative antigens	1225:1258	However, they did not improve protective efficacy in mice against challenge with M. tuberculosis compared to conventional vaccination with BCG, suggesting that additional or alternative antigens may be needed to protect against this disease.
29682888	4	37	from	structures	572:581	arg1	N. benthamiana					596:609	N. benthamiana	596:609	N. benthamiana	596:609	The recombinant protein assembled into polymeric structures (TB-PIGS) in N. benthamiana, similar in size to polymeric IgM.
29682888	3	38	theme	Ovary	475:479	arg1	cells					487:491	Chinese Hamster Ovary (CHO) cells	459:491	Chinese Hamster Ovary (CHO) cells	459:491	Expression was attempted in Chinese Hamster Ovary (CHO) cells and in Nicotiana benthamiana.
29682888	4	39	theme	recombinant	527:537	arg1	protein					539:545	The recombinant protein	523:545	The recombinant protein	523:545	The recombinant protein assembled into polymeric structures (TB-PIGS) in N. benthamiana, similar in size to polymeric IgM.
29682888	4	40	from	size	623:626	arg1	similar					612:618	similar	612:618	similar	612:618	The recombinant protein assembled into polymeric structures (TB-PIGS) in N. benthamiana, similar in size to polymeric IgM.
29682888	5	41	with	FcγRs	728:732	arg1	affinity					749:756	increased affinity	739:756	increased affinity	739:756	These complexes were subsequently shown to bind to the complement protein C1q and FcγRs with increased affinity.
29682888	2	42	theme	γ-chain	352:358	arg1	fragment					360:367	an immunoglobulin γ-chain fragment	334:367	an immunoglobulin γ-chain fragment	334:367	A genetic fusion construct comprising genes encoding the mycobacterial Ag85B antigen, an immunoglobulin γ-chain fragment and the tailpiece from immunoglobulin μ chain was engineered.
29682888	2	42	theme	γ-chain	352:358	arg1	antigen					325:331	the mycobacterial Ag85B antigen	301:331	the mycobacterial Ag85B antigen	301:331	A genetic fusion construct comprising genes encoding the mycobacterial Ag85B antigen, an immunoglobulin γ-chain fragment and the tailpiece from immunoglobulin μ chain was engineered.
29682888	9	43	from	vaccines	1479:1486	arg1	desirable					1466:1474	desirable	1466:1474	desirable	1466:1474	Nevertheless, these results establish a novel platform for producing polymeric antigen-IgG γ-chain molecules with inherent functional characteristics that are desirable in vaccines.
29682888	6	44	theme	low-affinity	943:954	arg1	FcγRs					956:960	low-affinity FcγRs	943:960	low-affinity FcγRs	943:960	Modification of the N-glycans linked to TB-PIGS by removal of xylose and fucose residues that are normally found in plant glycosylated proteins also resulted in increased affinity for low-affinity FcγRs.
29682888	8	45	theme	protective	1095:1104	arg1	efficacy					1106:1113	protective efficacy	1095:1113	protective efficacy in mice against challenge with M. tuberculosis compared to conventional vaccination with BCG, suggesting that additional or alternative antigens may be needed to protect against this disease	1095:1304	However, they did not improve protective efficacy in mice against challenge with M. tuberculosis compared to conventional vaccination with BCG, suggesting that additional or alternative antigens may be needed to protect against this disease.
29682888	6	46	theme	residues	839:846	arg1	removal					810:816	removal	810:816	removal of xylose and fucose residues that are normally found in plant glycosylated proteins	810:901	Modification of the N-glycans linked to TB-PIGS by removal of xylose and fucose residues that are normally found in plant glycosylated proteins also resulted in increased affinity for low-affinity FcγRs.
29682888	2	47	from	chain	409:413	arg1	tailpiece					377:385	the tailpiece	373:385	the tailpiece from immunoglobulin μ chain	373:413	A genetic fusion construct comprising genes encoding the mycobacterial Ag85B antigen, an immunoglobulin γ-chain fragment and the tailpiece from immunoglobulin μ chain was engineered.
29682888	2	47	from	chain	409:413	arg1	fragment					360:367	an immunoglobulin γ-chain fragment	334:367	an immunoglobulin γ-chain fragment	334:367	A genetic fusion construct comprising genes encoding the mycobacterial Ag85B antigen, an immunoglobulin γ-chain fragment and the tailpiece from immunoglobulin μ chain was engineered.
29682888	2	47	from	chain	409:413	arg1	antigen					325:331	the mycobacterial Ag85B antigen	301:331	the mycobacterial Ag85B antigen	301:331	A genetic fusion construct comprising genes encoding the mycobacterial Ag85B antigen, an immunoglobulin γ-chain fragment and the tailpiece from immunoglobulin μ chain was engineered.
29682888	2	48	theme	immunoglobulin	337:350	arg1	fragment					360:367	an immunoglobulin γ-chain fragment	334:367	an immunoglobulin γ-chain fragment	334:367	A genetic fusion construct comprising genes encoding the mycobacterial Ag85B antigen, an immunoglobulin γ-chain fragment and the tailpiece from immunoglobulin μ chain was engineered.
29682888	2	48	theme	immunoglobulin	337:350	arg1	antigen					325:331	the mycobacterial Ag85B antigen	301:331	the mycobacterial Ag85B antigen	301:331	A genetic fusion construct comprising genes encoding the mycobacterial Ag85B antigen, an immunoglobulin γ-chain fragment and the tailpiece from immunoglobulin μ chain was engineered.
29682888	8	49	with	vaccination	1187:1197	arg1	BCG					1204:1206	BCG	1204:1206	BCG	1204:1206	However, they did not improve protective efficacy in mice against challenge with M. tuberculosis compared to conventional vaccination with BCG, suggesting that additional or alternative antigens may be needed to protect against this disease.
29682888	1	50	used	used	180:183	arg2	strategy					114:121	a strategy	112:121	a strategy based on polymeric immunoglobulin G scaffolds (PIGS)	112:174	In this study, a strategy based on polymeric immunoglobulin G scaffolds (PIGS) was used to produce a vaccine candidate for Mycobacterium tuberculosis.
29682888	2	51	theme	fusion	258:263	arg1	construct					265:273	A genetic fusion construct	248:273	A genetic fusion construct comprising genes encoding the mycobacterial Ag85B antigen, an immunoglobulin γ-chain fragment and the tailpiece from immunoglobulin μ chain	248:413	A genetic fusion construct comprising genes encoding the mycobacterial Ag85B antigen, an immunoglobulin γ-chain fragment and the tailpiece from immunoglobulin μ chain was engineered.
29682888	6	52	dep	glycosylated	881:892	arg1	plant					875:879	plant	875:879	plant	875:879	Modification of the N-glycans linked to TB-PIGS by removal of xylose and fucose residues that are normally found in plant glycosylated proteins also resulted in increased affinity for low-affinity FcγRs.
29682888	6	53	theme	fucose	832:837	arg1	residues					839:846	xylose and fucose residues	821:846	xylose and fucose residues that are normally found in plant glycosylated proteins	821:901	Modification of the N-glycans linked to TB-PIGS by removal of xylose and fucose residues that are normally found in plant glycosylated proteins also resulted in increased affinity for low-affinity FcγRs.
29682888	2	54	theme	genetic	250:256	arg1	construct					265:273	A genetic fusion construct	248:273	A genetic fusion construct comprising genes encoding the mycobacterial Ag85B antigen, an immunoglobulin γ-chain fragment and the tailpiece from immunoglobulin μ chain	248:413	A genetic fusion construct comprising genes encoding the mycobacterial Ag85B antigen, an immunoglobulin γ-chain fragment and the tailpiece from immunoglobulin μ chain was engineered.
29682888	1	55	theme	polymeric	132:140	arg1	PIGS					170:173	PIGS	170:173	PIGS	170:173	In this study, a strategy based on polymeric immunoglobulin G scaffolds (PIGS) was used to produce a vaccine candidate for Mycobacterium tuberculosis.
29682888	1	55	theme	polymeric	132:140	arg1	scaffolds					159:167	polymeric immunoglobulin G scaffolds	132:167	polymeric immunoglobulin G scaffolds (PIGS)	132:174	In this study, a strategy based on polymeric immunoglobulin G scaffolds (PIGS) was used to produce a vaccine candidate for Mycobacterium tuberculosis.
29682888	8	56	from	efficacy	1106:1113	arg1	mice					1118:1121	mice	1118:1121	mice	1118:1121	However, they did not improve protective efficacy in mice against challenge with M. tuberculosis compared to conventional vaccination with BCG, suggesting that additional or alternative antigens may be needed to protect against this disease.
29682888	1	57	theme	vaccine	198:204	arg1	candidate					206:214	a vaccine candidate	196:214	a vaccine candidate for Mycobacterium tuberculosis	196:245	In this study, a strategy based on polymeric immunoglobulin G scaffolds (PIGS) was used to produce a vaccine candidate for Mycobacterium tuberculosis.
29682888	1	58	theme	immunoglobulin	142:155	arg1	PIGS					170:173	PIGS	170:173	PIGS	170:173	In this study, a strategy based on polymeric immunoglobulin G scaffolds (PIGS) was used to produce a vaccine candidate for Mycobacterium tuberculosis.
29682888	1	58	theme	immunoglobulin	142:155	arg1	scaffolds					159:167	polymeric immunoglobulin G scaffolds	132:167	polymeric immunoglobulin G scaffolds (PIGS)	132:174	In this study, a strategy based on polymeric immunoglobulin G scaffolds (PIGS) was used to produce a vaccine candidate for Mycobacterium tuberculosis.
29682888	3	59	theme	Chinese	459:465	arg1	cells					487:491	Chinese Hamster Ovary (CHO) cells	459:491	Chinese Hamster Ovary (CHO) cells	459:491	Expression was attempted in Chinese Hamster Ovary (CHO) cells and in Nicotiana benthamiana.
29682888	1	60	theme	Mycobacterium	220:232	arg1	tuberculosis					234:245	Mycobacterium tuberculosis	220:245	Mycobacterium tuberculosis	220:245	In this study, a strategy based on polymeric immunoglobulin G scaffolds (PIGS) was used to produce a vaccine candidate for Mycobacterium tuberculosis.
29682888	0	61	theme	vaccine	73:79	arg1	immunogenicity					81:94	vaccine immunogenicity	73:94	vaccine immunogenicity	73:94	A polymeric immunoglobulin-antigen fusion protein strategy for enhancing vaccine immunogenicity.
29682888	8	62	theme	additional	1225:1234	arg1	antigens					1251:1258	additional or alternative antigens	1225:1258	additional or alternative antigens	1225:1258	However, they did not improve protective efficacy in mice against challenge with M. tuberculosis compared to conventional vaccination with BCG, suggesting that additional or alternative antigens may be needed to protect against this disease.
29682888	1	63	theme	G	157:157	arg1	PIGS					170:173	PIGS	170:173	PIGS	170:173	In this study, a strategy based on polymeric immunoglobulin G scaffolds (PIGS) was used to produce a vaccine candidate for Mycobacterium tuberculosis.
29682888	1	63	theme	G	157:157	arg1	scaffolds					159:167	polymeric immunoglobulin G scaffolds	132:167	polymeric immunoglobulin G scaffolds (PIGS)	132:174	In this study, a strategy based on polymeric immunoglobulin G scaffolds (PIGS) was used to produce a vaccine candidate for Mycobacterium tuberculosis.
30415057	9	0	theme	anti-inflammatory	597:613	arg1	activity					615:622	similar anti-inflammatory activity	589:622	similar anti-inflammatory activity	589:622	AIM Thus, the aim of this study is to investigate if the aqueous extract of MG and ML present similar anti-inflammatory activity to the point of being used interchangeably.
30415057	7	1	theme	chemical	326:333	arg1	composition					335:345	chemical composition	326:345	chemical composition	326:345	ex Baker (ML), popularly known as guaco, are medicinal plants similar in morphology, chemical composition and medicinal uses.
30415057	15	2	theme	histamine	1636:1644	arg1	release					1646:1652	the consequent histamine release	1621:1652	the consequent histamine release	1621:1652	MG, ML and coumarin did not prevent mast cell degranulation and the consequent histamine release in Wistar rat peritoneal mast cells induced by compound 48/80.
30415057	7	3	theme	similar	303:309	arg1	plants					296:301	medicinal plants	286:301	medicinal plants similar in morphology, chemical composition and medicinal uses	286:364	ex Baker (ML), popularly known as guaco, are medicinal plants similar in morphology, chemical composition and medicinal uses.
30415057	7	3	theme	similar	303:309	arg1	Baker					244:248	ex Baker	241:248	ex Baker (ML)	241:253	ex Baker (ML), popularly known as guaco, are medicinal plants similar in morphology, chemical composition and medicinal uses.
30415057	16	4	dep	%	1860:1860	arg1	p < 0.001					1882:1890	p < 0.001	1882:1890	p < 0.001	1882:1890	MG did not inhibit cell infiltration in pleurisy nor the highest dose tested, while ML decreased the leukocyte migration (200 and 400 mg/kg, 23% and 30% inhibition; p < 0.001, respectively) and, to a lesser extent, coumarin also reduced cell infiltration (10, 50 and 75 mg/kg; 15%, 16% and 17% inhibition; p < 0.001, respectively).
30415057	10	5	theme	METHODS	681:687	arg1	doses					699:703	MATERIAL AND METHODS Different doses	668:703	MATERIAL AND METHODS Different doses of both extracts and coumarin	668:733	MATERIAL AND METHODS Different doses of both extracts and coumarin were given to rats in different experimental models to assess the anti-inflammatory activity between these two species.
30415057	10	6	dep	models	780:785	arg1	assess					790:795	assess	790:795	to assess the anti-inflammatory activity between these two species	787:852	MATERIAL AND METHODS Different doses of both extracts and coumarin were given to rats in different experimental models to assess the anti-inflammatory activity between these two species.
30415057	7	7	theme	medicinal	286:294	arg1	plants					296:301	medicinal plants	286:301	medicinal plants similar in morphology, chemical composition and medicinal uses	286:364	ex Baker (ML), popularly known as guaco, are medicinal plants similar in morphology, chemical composition and medicinal uses.
30415057	7	7	theme	medicinal	286:294	arg1	Baker					244:248	ex Baker	241:248	ex Baker (ML)	241:253	ex Baker (ML), popularly known as guaco, are medicinal plants similar in morphology, chemical composition and medicinal uses.
30415057	8	8	theme	chemical	460:467	arg1	composition					469:479	their chemical composition	454:479	their chemical composition	454:479	Both species are often used and sold without distinction; however, it is believed that their chemical composition is different.
30415057	17	9	theme	M.	2149:2150	arg1	laevigata					2152:2160	M. laevigata	2149:2160	M. laevigata	2149:2160	CONCLUSION The variation of the results of the anti-inflammatory activity found in M. glomerata and M. laevigata demonstrates that these two species should not be used interchangeably.
30415057	17	10	located	found	2123:2127	arg2	activity					2114:2121	the anti-inflammatory activity	2092:2121	the anti-inflammatory activity found in M. glomerata and M. laevigata	2092:2160	CONCLUSION The variation of the results of the anti-inflammatory activity found in M. glomerata and M. laevigata demonstrates that these two species should not be used interchangeably.
30415057	17	10	located	found	2123:2127	arg1	glomerata					2135:2143	M. glomerata	2132:2143	M. glomerata	2132:2143	CONCLUSION The variation of the results of the anti-inflammatory activity found in M. glomerata and M. laevigata demonstrates that these two species should not be used interchangeably.
30415057	17	10	located	found	2123:2127	arg1	laevigata					2152:2160	M. laevigata	2149:2160	M. laevigata	2149:2160	CONCLUSION The variation of the results of the anti-inflammatory activity found in M. glomerata and M. laevigata demonstrates that these two species should not be used interchangeably.
30415057	16	11	dep	10	1973:1974	arg1	%					1996:1996	15%	1994:1996	15%	1994:1996	MG did not inhibit cell infiltration in pleurisy nor the highest dose tested, while ML decreased the leukocyte migration (200 and 400 mg/kg, 23% and 30% inhibition; p < 0.001, respectively) and, to a lesser extent, coumarin also reduced cell infiltration (10, 50 and 75 mg/kg; 15%, 16% and 17% inhibition; p < 0.001, respectively).
30415057	16	11	dep	10	1973:1974	arg1	%					2001:2001	16%	1999:2001	16%	1999:2001	MG did not inhibit cell infiltration in pleurisy nor the highest dose tested, while ML decreased the leukocyte migration (200 and 400 mg/kg, 23% and 30% inhibition; p < 0.001, respectively) and, to a lesser extent, coumarin also reduced cell infiltration (10, 50 and 75 mg/kg; 15%, 16% and 17% inhibition; p < 0.001, respectively).
30415057	16	11	dep	10	1973:1974	arg1	p < 0.001					2023:2031	p < 0.001	2023:2031	p < 0.001	2023:2031	MG did not inhibit cell infiltration in pleurisy nor the highest dose tested, while ML decreased the leukocyte migration (200 and 400 mg/kg, 23% and 30% inhibition; p < 0.001, respectively) and, to a lesser extent, coumarin also reduced cell infiltration (10, 50 and 75 mg/kg; 15%, 16% and 17% inhibition; p < 0.001, respectively).
30415057	16	11	dep	10	1973:1974	arg1	inhibition					2011:2020	17% inhibition	2007:2020	17% inhibition	2007:2020	MG did not inhibit cell infiltration in pleurisy nor the highest dose tested, while ML decreased the leukocyte migration (200 and 400 mg/kg, 23% and 30% inhibition; p < 0.001, respectively) and, to a lesser extent, coumarin also reduced cell infiltration (10, 50 and 75 mg/kg; 15%, 16% and 17% inhibition; p < 0.001, respectively).
30415057	11	12	theme	Efficiency	1012:1021	arg1	Chromatography					1030:1043	Ultra High Efficiency Liquid Chromatography	1001:1043	Ultra High Efficiency Liquid Chromatography coupled to Mass Spectrometry (UHPLC-MS)	1001:1083	For this, the animals were submitted to paw edema, pleurisy and degranulation of peritoneal mast cell and the extracts were also characterized by Ultra High Efficiency Liquid Chromatography coupled to Mass Spectrometry (UHPLC-MS).
30415057	14	13	theme	%	1518:1518	arg1	inhibition					1520:1529	40% inhibition	1516:1529	40% inhibition	1516:1529	MG, ML and coumarin treatment also inhibited the edema induced by compound 48/80 (400 mg/kg, 56% inhibition; 400 mg/kg, 69% inhibition; 75 mg/kg, 40% inhibition; p < 0.05, respectively).
30415057	11	14	theme	Ultra	1001:1005	arg1	Chromatography					1030:1043	Ultra High Efficiency Liquid Chromatography	1001:1043	Ultra High Efficiency Liquid Chromatography coupled to Mass Spectrometry (UHPLC-MS)	1001:1083	For this, the animals were submitted to paw edema, pleurisy and degranulation of peritoneal mast cell and the extracts were also characterized by Ultra High Efficiency Liquid Chromatography coupled to Mass Spectrometry (UHPLC-MS).
30415057	4	15	theme	ETHNOPHARMACOLOGICAL	147:166	arg1	RELEVANCE					168:176	ETHNOPHARMACOLOGICAL RELEVANCE	147:176	ETHNOPHARMACOLOGICAL RELEVANCE	147:176	ETHNOPHARMACOLOGICAL RELEVANCE Mikania glomerata Spreng.
30415057	15	16	theme	mast	1679:1682	arg1	cells					1684:1688	Wistar rat peritoneal mast cells	1657:1688	Wistar rat peritoneal mast cells induced by compound 48/80	1657:1714	MG, ML and coumarin did not prevent mast cell degranulation and the consequent histamine release in Wistar rat peritoneal mast cells induced by compound 48/80.
30415057	11	17	theme	peritoneal	936:945	arg1	cell					952:955	peritoneal mast cell	936:955	peritoneal mast cell	936:955	For this, the animals were submitted to paw edema, pleurisy and degranulation of peritoneal mast cell and the extracts were also characterized by Ultra High Efficiency Liquid Chromatography coupled to Mass Spectrometry (UHPLC-MS).
30415057	10	18	theme	experimental	767:778	arg1	models					780:785	different experimental models	757:785	different experimental models to assess the anti-inflammatory activity between these two species	757:852	MATERIAL AND METHODS Different doses of both extracts and coumarin were given to rats in different experimental models to assess the anti-inflammatory activity between these two species.
30415057	3	19	theme	ex	136:137	arg1	Baker					139:143	ex Baker	136:143	ex Baker	136:143	ex Baker).
30415057	18	20	theme	responsible	2362:2372	arg1	it					2324:2325	it	2324:2325	it	2324:2325	Coumarin, as already proven, has anti-inflammatory action however, we have suggested that it probably is not the only component responsible for this therapeutic effect in the extracts.
30415057	18	20	theme	responsible	2362:2372	arg1	component					2352:2360	the only component	2343:2360	the only component responsible for this therapeutic effect in the extracts	2343:2416	Coumarin, as already proven, has anti-inflammatory action however, we have suggested that it probably is not the only component responsible for this therapeutic effect in the extracts.
30415057	12	21	theme	chromatographic	1098:1112	arg1	method					1114:1119	The chromatographic method	1094:1119	The chromatographic method	1094:1119	RESULTS The chromatographic method showed that ML presents ten times more coumarin than MG.
30415057	14	22	theme	%	1492:1492	arg1	inhibition					1494:1503	69% inhibition	1490:1503	69% inhibition	1490:1503	MG, ML and coumarin treatment also inhibited the edema induced by compound 48/80 (400 mg/kg, 56% inhibition; 400 mg/kg, 69% inhibition; 75 mg/kg, 40% inhibition; p < 0.05, respectively).
30415057	15	23	from	degranulation	1603:1615	arg1	cells					1684:1688	Wistar rat peritoneal mast cells	1657:1688	Wistar rat peritoneal mast cells induced by compound 48/80	1657:1714	MG, ML and coumarin did not prevent mast cell degranulation and the consequent histamine release in Wistar rat peritoneal mast cells induced by compound 48/80.
30415057	5	24	dep	MG	205:206	arg1	Mikania					213:219	(MG) and Mikania laevigata Sch	204:233	Mikania	213:219	(MG) and Mikania laevigata Sch.
30415057	0	25	theme	glomerata	83:91	arg1	extracts					63:70	aqueous extracts	55:70	aqueous extracts of Mikania glomerata	55:91	Pharmacological study of anti-inflammatory activity of aqueous extracts of Mikania glomerata (Spreng.)
30415057	11	26	theme	cell	952:955	arg1	edema					899:903	paw edema	895:903	paw edema	895:903	For this, the animals were submitted to paw edema, pleurisy and degranulation of peritoneal mast cell and the extracts were also characterized by Ultra High Efficiency Liquid Chromatography coupled to Mass Spectrometry (UHPLC-MS).
30415057	11	26	theme	cell	952:955	arg1	degranulation					919:931	degranulation	919:931	degranulation	919:931	For this, the animals were submitted to paw edema, pleurisy and degranulation of peritoneal mast cell and the extracts were also characterized by Ultra High Efficiency Liquid Chromatography coupled to Mass Spectrometry (UHPLC-MS).
30415057	11	26	theme	cell	952:955	arg1	pleurisy					906:913	pleurisy	906:913	pleurisy	906:913	For this, the animals were submitted to paw edema, pleurisy and degranulation of peritoneal mast cell and the extracts were also characterized by Ultra High Efficiency Liquid Chromatography coupled to Mass Spectrometry (UHPLC-MS).
30415057	9	27	dep	Thus	499:502	arg1	AIM					495:497	AIM	495:497	AIM	495:497	AIM Thus, the aim of this study is to investigate if the aqueous extract of MG and ML present similar anti-inflammatory activity to the point of being used interchangeably.
30415057	16	28	theme	%	2009:2009	arg1	inhibition					2011:2020	17% inhibition	2007:2020	17% inhibition	2007:2020	MG did not inhibit cell infiltration in pleurisy nor the highest dose tested, while ML decreased the leukocyte migration (200 and 400 mg/kg, 23% and 30% inhibition; p < 0.001, respectively) and, to a lesser extent, coumarin also reduced cell infiltration (10, 50 and 75 mg/kg; 15%, 16% and 17% inhibition; p < 0.001, respectively).
30415057	13	29	theme	paw	1231:1233	arg1	edema					1235:1239	paw edema	1231:1239	paw edema induced by carrageenan (400 mg/kg, 55% inhibition; 400 mg/kg, 57% inhibition; 75 mg/kg, 38% inhibition; p < 0.05, respectively)	1231:1367	Oral administration of MG, ML and coumarin inhibited paw edema induced by carrageenan (400 mg/kg, 55% inhibition; 400 mg/kg, 57% inhibition; 75 mg/kg, 38% inhibition; p < 0.05, respectively).
30415057	4	30	dep	Spreng	196:201	arg1	RELEVANCE					168:176	ETHNOPHARMACOLOGICAL RELEVANCE	147:176	ETHNOPHARMACOLOGICAL RELEVANCE	147:176	ETHNOPHARMACOLOGICAL RELEVANCE Mikania glomerata Spreng.
30415057	14	31	theme	%	1465:1465	arg1	inhibition					1467:1476	56% inhibition	1463:1476	56% inhibition	1463:1476	MG, ML and coumarin treatment also inhibited the edema induced by compound 48/80 (400 mg/kg, 56% inhibition; 400 mg/kg, 69% inhibition; 75 mg/kg, 40% inhibition; p < 0.05, respectively).
30415057	16	32	theme	highest	1774:1780	arg1	dose					1782:1785	the highest dose	1770:1785	the highest dose tested, while ML decreased the leukocyte migration (200 and 400 mg/kg, 23% and 30% inhibition; p < 0.001, respectively) and, to a lesser extent, coumarin	1770:1939	MG did not inhibit cell infiltration in pleurisy nor the highest dose tested, while ML decreased the leukocyte migration (200 and 400 mg/kg, 23% and 30% inhibition; p < 0.001, respectively) and, to a lesser extent, coumarin also reduced cell infiltration (10, 50 and 75 mg/kg; 15%, 16% and 17% inhibition; p < 0.001, respectively).
30415057	16	33	dep	infiltration	1959:1970	arg1	75 mg/kg					1984:1991	75 mg/kg	1984:1991	75 mg/kg	1984:1991	MG did not inhibit cell infiltration in pleurisy nor the highest dose tested, while ML decreased the leukocyte migration (200 and 400 mg/kg, 23% and 30% inhibition; p < 0.001, respectively) and, to a lesser extent, coumarin also reduced cell infiltration (10, 50 and 75 mg/kg; 15%, 16% and 17% inhibition; p < 0.001, respectively).
30415057	16	33	dep	infiltration	1959:1970	arg1	10					1973:1974	10	1973:1974	10	1973:1974	MG did not inhibit cell infiltration in pleurisy nor the highest dose tested, while ML decreased the leukocyte migration (200 and 400 mg/kg, 23% and 30% inhibition; p < 0.001, respectively) and, to a lesser extent, coumarin also reduced cell infiltration (10, 50 and 75 mg/kg; 15%, 16% and 17% inhibition; p < 0.001, respectively).
30415057	15	34	theme	mast	1593:1596	arg1	degranulation					1603:1615	mast cell degranulation	1593:1615	mast cell degranulation	1593:1615	MG, ML and coumarin did not prevent mast cell degranulation and the consequent histamine release in Wistar rat peritoneal mast cells induced by compound 48/80.
30415057	0	35	theme	Pharmacological	0:14	arg1	study					16:20	Pharmacological study	0:20	Pharmacological study of anti-inflammatory activity of aqueous extracts of Mikania glomerata (Spreng.)	0:101	Pharmacological study of anti-inflammatory activity of aqueous extracts of Mikania glomerata (Spreng.)
30415057	0	35	theme	Pharmacological	0:14	arg1	Spreng					94:99	Spreng	94:99	Spreng	94:99	Pharmacological study of anti-inflammatory activity of aqueous extracts of Mikania glomerata (Spreng.)
30415057	18	36	dep	Coumarin	2234:2241	arg1	proven					2255:2260	proven	2255:2260	proven	2255:2260	Coumarin, as already proven, has anti-inflammatory action however, we have suggested that it probably is not the only component responsible for this therapeutic effect in the extracts.
30415057	18	37	from	effect	2395:2400	arg1	extracts					2409:2416	the extracts	2405:2416	the extracts	2405:2416	Coumarin, as already proven, has anti-inflammatory action however, we have suggested that it probably is not the only component responsible for this therapeutic effect in the extracts.
30415057	18	38	theme	only	2347:2350	arg1	it					2324:2325	it	2324:2325	it	2324:2325	Coumarin, as already proven, has anti-inflammatory action however, we have suggested that it probably is not the only component responsible for this therapeutic effect in the extracts.
30415057	18	38	theme	only	2347:2350	arg1	component					2352:2360	the only component	2343:2360	the only component responsible for this therapeutic effect in the extracts	2343:2416	Coumarin, as already proven, has anti-inflammatory action however, we have suggested that it probably is not the only component responsible for this therapeutic effect in the extracts.
30415057	10	39	theme	MATERIAL	668:675	arg1	doses					699:703	MATERIAL AND METHODS Different doses	668:703	MATERIAL AND METHODS Different doses of both extracts and coumarin	668:733	MATERIAL AND METHODS Different doses of both extracts and coumarin were given to rats in different experimental models to assess the anti-inflammatory activity between these two species.
30415057	13	40	dep	carrageenan	1252:1262	arg1	400 mg/kg					1265:1273	400 mg/kg	1265:1273	400 mg/kg	1265:1273	Oral administration of MG, ML and coumarin inhibited paw edema induced by carrageenan (400 mg/kg, 55% inhibition; 400 mg/kg, 57% inhibition; 75 mg/kg, 38% inhibition; p < 0.05, respectively).
30415057	17	41	theme	results	2081:2087	arg1	variation					2064:2072	The variation	2060:2072	The variation of the results of the anti-inflammatory activity found in M. glomerata and M. laevigata	2060:2160	CONCLUSION The variation of the results of the anti-inflammatory activity found in M. glomerata and M. laevigata demonstrates that these two species should not be used interchangeably.
30415057	7	42	from	similar	303:309	arg1	medicinal					351:359	medicinal	351:359	medicinal	351:359	ex Baker (ML), popularly known as guaco, are medicinal plants similar in morphology, chemical composition and medicinal uses.
30415057	7	42	from	similar	303:309	arg1	morphology					314:323	morphology	314:323	morphology	314:323	ex Baker (ML), popularly known as guaco, are medicinal plants similar in morphology, chemical composition and medicinal uses.
30415057	7	42	from	similar	303:309	arg1	composition					335:345	chemical composition	326:345	chemical composition	326:345	ex Baker (ML), popularly known as guaco, are medicinal plants similar in morphology, chemical composition and medicinal uses.
30415057	0	43	theme	activity	43:50	arg1	study					16:20	Pharmacological study	0:20	Pharmacological study of anti-inflammatory activity of aqueous extracts of Mikania glomerata (Spreng.)	0:101	Pharmacological study of anti-inflammatory activity of aqueous extracts of Mikania glomerata (Spreng.)
30415057	0	43	theme	activity	43:50	arg1	Spreng					94:99	Spreng	94:99	Spreng	94:99	Pharmacological study of anti-inflammatory activity of aqueous extracts of Mikania glomerata (Spreng.)
30415057	5	44	theme	laevigata	221:229	arg1	Sch					231:233	(MG) and Mikania laevigata Sch	204:233	Sch	231:233	(MG) and Mikania laevigata Sch.
30415057	16	45	theme	cell	1954:1957	arg1	infiltration					1959:1970	cell infiltration	1954:1970	cell infiltration (10, 50 and 75 mg/kg; 15%, 16% and 17% inhibition; p < 0.001, respectively)	1954:2046	MG did not inhibit cell infiltration in pleurisy nor the highest dose tested, while ML decreased the leukocyte migration (200 and 400 mg/kg, 23% and 30% inhibition; p < 0.001, respectively) and, to a lesser extent, coumarin also reduced cell infiltration (10, 50 and 75 mg/kg; 15%, 16% and 17% inhibition; p < 0.001, respectively).
30415057	16	46	theme	200	1839:1841	arg1	%					1860:1860	200 and 400 mg/kg, 23% and 30% inhibition	1839:1879	%	1860:1860	MG did not inhibit cell infiltration in pleurisy nor the highest dose tested, while ML decreased the leukocyte migration (200 and 400 mg/kg, 23% and 30% inhibition; p < 0.001, respectively) and, to a lesser extent, coumarin also reduced cell infiltration (10, 50 and 75 mg/kg; 15%, 16% and 17% inhibition; p < 0.001, respectively).
30415057	7	47	from	morphology	314:323	arg1	similar					303:309	similar	303:309	similar	303:309	ex Baker (ML), popularly known as guaco, are medicinal plants similar in morphology, chemical composition and medicinal uses.
30415057	0	48	theme	aqueous	55:61	arg1	extracts					63:70	aqueous extracts	55:70	aqueous extracts of Mikania glomerata	55:91	Pharmacological study of anti-inflammatory activity of aqueous extracts of Mikania glomerata (Spreng.)
30415057	11	49	theme	paw	895:897	arg1	edema					899:903	paw edema	895:903	paw edema	895:903	For this, the animals were submitted to paw edema, pleurisy and degranulation of peritoneal mast cell and the extracts were also characterized by Ultra High Efficiency Liquid Chromatography coupled to Mass Spectrometry (UHPLC-MS).
30415057	11	49	theme	paw	895:897	arg1	degranulation					919:931	degranulation	919:931	degranulation	919:931	For this, the animals were submitted to paw edema, pleurisy and degranulation of peritoneal mast cell and the extracts were also characterized by Ultra High Efficiency Liquid Chromatography coupled to Mass Spectrometry (UHPLC-MS).
30415057	11	49	theme	paw	895:897	arg1	pleurisy					906:913	pleurisy	906:913	pleurisy	906:913	For this, the animals were submitted to paw edema, pleurisy and degranulation of peritoneal mast cell and the extracts were also characterized by Ultra High Efficiency Liquid Chromatography coupled to Mass Spectrometry (UHPLC-MS).
30415057	17	50	dep	CONCLUSION	2049:2058	arg1	demonstrates					2162:2173	demonstrates	2162:2173	demonstrates that these two species should not be used interchangeably	2162:2231	CONCLUSION The variation of the results of the anti-inflammatory activity found in M. glomerata and M. laevigata demonstrates that these two species should not be used interchangeably.
30415057	9	51	theme	MG	571:572	arg1	extract					560:566	the aqueous extract	548:566	the aqueous extract of MG and ML	548:579	AIM Thus, the aim of this study is to investigate if the aqueous extract of MG and ML present similar anti-inflammatory activity to the point of being used interchangeably.
30415057	17	52	theme	activity	2114:2121	arg1	results					2081:2087	the results	2077:2087	the results of the anti-inflammatory activity found in M. glomerata and M. laevigata	2077:2160	CONCLUSION The variation of the results of the anti-inflammatory activity found in M. glomerata and M. laevigata demonstrates that these two species should not be used interchangeably.
30415057	16	53	theme	400 mg/kg	1847:1855	arg1	%					1860:1860	200 and 400 mg/kg, 23% and 30% inhibition	1839:1879	%	1860:1860	MG did not inhibit cell infiltration in pleurisy nor the highest dose tested, while ML decreased the leukocyte migration (200 and 400 mg/kg, 23% and 30% inhibition; p < 0.001, respectively) and, to a lesser extent, coumarin also reduced cell infiltration (10, 50 and 75 mg/kg; 15%, 16% and 17% inhibition; p < 0.001, respectively).
30415057	13	54	theme	Oral	1178:1181	arg1	administration					1183:1196	Oral administration	1178:1196	Oral administration of MG, ML and coumarin	1178:1219	Oral administration of MG, ML and coumarin inhibited paw edema induced by carrageenan (400 mg/kg, 55% inhibition; 400 mg/kg, 57% inhibition; 75 mg/kg, 38% inhibition; p < 0.05, respectively).
30415057	5	55	theme	MG	205:206	arg1	Sch					231:233	(MG) and Mikania laevigata Sch	204:233	Sch	231:233	(MG) and Mikania laevigata Sch.
30415057	16	56	theme	cell	1736:1739	arg1	infiltration					1741:1752	cell infiltration	1736:1752	cell infiltration in pleurisy	1736:1764	MG did not inhibit cell infiltration in pleurisy nor the highest dose tested, while ML decreased the leukocyte migration (200 and 400 mg/kg, 23% and 30% inhibition; p < 0.001, respectively) and, to a lesser extent, coumarin also reduced cell infiltration (10, 50 and 75 mg/kg; 15%, 16% and 17% inhibition; p < 0.001, respectively).
30415057	9	57	theme	ML	578:579	arg1	extract					560:566	the aqueous extract	548:566	the aqueous extract of MG and ML	548:579	AIM Thus, the aim of this study is to investigate if the aqueous extract of MG and ML present similar anti-inflammatory activity to the point of being used interchangeably.
30415057	15	58	theme	Wistar	1657:1662	arg1	cells					1684:1688	Wistar rat peritoneal mast cells	1657:1688	Wistar rat peritoneal mast cells induced by compound 48/80	1657:1714	MG, ML and coumarin did not prevent mast cell degranulation and the consequent histamine release in Wistar rat peritoneal mast cells induced by compound 48/80.
30415057	9	59	theme	similar	589:595	arg1	activity					615:622	similar anti-inflammatory activity	589:622	similar anti-inflammatory activity	589:622	AIM Thus, the aim of this study is to investigate if the aqueous extract of MG and ML present similar anti-inflammatory activity to the point of being used interchangeably.
30415057	18	60	theme	anti-inflammatory	2267:2283	arg1	action					2285:2290	anti-inflammatory action	2267:2290	anti-inflammatory action	2267:2290	Coumarin, as already proven, has anti-inflammatory action however, we have suggested that it probably is not the only component responsible for this therapeutic effect in the extracts.
30415057	14	61	dep	400 mg/kg	1452:1460	arg1	inhibition					1520:1529	40% inhibition	1516:1529	40% inhibition	1516:1529	MG, ML and coumarin treatment also inhibited the edema induced by compound 48/80 (400 mg/kg, 56% inhibition; 400 mg/kg, 69% inhibition; 75 mg/kg, 40% inhibition; p < 0.05, respectively).
30415057	14	61	dep	400 mg/kg	1452:1460	arg1	inhibition					1467:1476	56% inhibition	1463:1476	56% inhibition	1463:1476	MG, ML and coumarin treatment also inhibited the edema induced by compound 48/80 (400 mg/kg, 56% inhibition; 400 mg/kg, 69% inhibition; 75 mg/kg, 40% inhibition; p < 0.05, respectively).
30415057	14	61	dep	400 mg/kg	1452:1460	arg1	inhibition					1494:1503	69% inhibition	1490:1503	69% inhibition	1490:1503	MG, ML and coumarin treatment also inhibited the edema induced by compound 48/80 (400 mg/kg, 56% inhibition; 400 mg/kg, 69% inhibition; 75 mg/kg, 40% inhibition; p < 0.05, respectively).
30415057	14	61	dep	400 mg/kg	1452:1460	arg1	75 mg/kg					1506:1513	75 mg/kg	1506:1513	75 mg/kg	1506:1513	MG, ML and coumarin treatment also inhibited the edema induced by compound 48/80 (400 mg/kg, 56% inhibition; 400 mg/kg, 69% inhibition; 75 mg/kg, 40% inhibition; p < 0.05, respectively).
30415057	14	61	dep	400 mg/kg	1452:1460	arg1	p < 0.05					1532:1539	p < 0.05	1532:1539	p < 0.05	1532:1539	MG, ML and coumarin treatment also inhibited the edema induced by compound 48/80 (400 mg/kg, 56% inhibition; 400 mg/kg, 69% inhibition; 75 mg/kg, 40% inhibition; p < 0.05, respectively).
30415057	14	61	dep	400 mg/kg	1452:1460	arg1	400 mg/kg					1479:1487	400 mg/kg	1479:1487	400 mg/kg	1479:1487	MG, ML and coumarin treatment also inhibited the edema induced by compound 48/80 (400 mg/kg, 56% inhibition; 400 mg/kg, 69% inhibition; 75 mg/kg, 40% inhibition; p < 0.05, respectively).
30415057	15	62	theme	consequent	1625:1634	arg1	release					1646:1652	the consequent histamine release	1621:1652	the consequent histamine release	1621:1652	MG, ML and coumarin did not prevent mast cell degranulation and the consequent histamine release in Wistar rat peritoneal mast cells induced by compound 48/80.
30415057	7	63	dep	medicinal	351:359	arg1	uses					361:364	uses	361:364	uses	361:364	ex Baker (ML), popularly known as guaco, are medicinal plants similar in morphology, chemical composition and medicinal uses.
30415057	10	64	theme	anti-inflammatory	801:817	arg1	activity					819:826	the anti-inflammatory activity	797:826	the anti-inflammatory activity between these two species	797:852	MATERIAL AND METHODS Different doses of both extracts and coumarin were given to rats in different experimental models to assess the anti-inflammatory activity between these two species.
30415057	13	65	dep	400 mg/kg	1265:1273	arg1	inhibition					1307:1316	57% inhibition	1303:1316	57% inhibition	1303:1316	Oral administration of MG, ML and coumarin inhibited paw edema induced by carrageenan (400 mg/kg, 55% inhibition; 400 mg/kg, 57% inhibition; 75 mg/kg, 38% inhibition; p < 0.05, respectively).
30415057	13	65	dep	400 mg/kg	1265:1273	arg1	inhibition					1333:1342	38% inhibition	1329:1342	38% inhibition	1329:1342	Oral administration of MG, ML and coumarin inhibited paw edema induced by carrageenan (400 mg/kg, 55% inhibition; 400 mg/kg, 57% inhibition; 75 mg/kg, 38% inhibition; p < 0.05, respectively).
30415057	13	65	dep	400 mg/kg	1265:1273	arg1	inhibition					1280:1289	55% inhibition	1276:1289	55% inhibition	1276:1289	Oral administration of MG, ML and coumarin inhibited paw edema induced by carrageenan (400 mg/kg, 55% inhibition; 400 mg/kg, 57% inhibition; 75 mg/kg, 38% inhibition; p < 0.05, respectively).
30415057	13	65	dep	400 mg/kg	1265:1273	arg1	p < 0.05					1345:1352	p < 0.05	1345:1352	p < 0.05	1345:1352	Oral administration of MG, ML and coumarin inhibited paw edema induced by carrageenan (400 mg/kg, 55% inhibition; 400 mg/kg, 57% inhibition; 75 mg/kg, 38% inhibition; p < 0.05, respectively).
30415057	13	65	dep	400 mg/kg	1265:1273	arg1	400 mg/kg					1292:1300	400 mg/kg	1292:1300	400 mg/kg	1292:1300	Oral administration of MG, ML and coumarin inhibited paw edema induced by carrageenan (400 mg/kg, 55% inhibition; 400 mg/kg, 57% inhibition; 75 mg/kg, 38% inhibition; p < 0.05, respectively).
30415057	13	65	dep	400 mg/kg	1265:1273	arg1	75 mg/kg					1319:1326	75 mg/kg	1319:1326	75 mg/kg	1319:1326	Oral administration of MG, ML and coumarin inhibited paw edema induced by carrageenan (400 mg/kg, 55% inhibition; 400 mg/kg, 57% inhibition; 75 mg/kg, 38% inhibition; p < 0.05, respectively).
30415057	13	65	dep	400 mg/kg	1265:1273	arg1	respectively					1355:1366	respectively	1355:1366	respectively	1355:1366	Oral administration of MG, ML and coumarin inhibited paw edema induced by carrageenan (400 mg/kg, 55% inhibition; 400 mg/kg, 57% inhibition; 75 mg/kg, 38% inhibition; p < 0.05, respectively).
30415057	13	66	theme	coumarin	1212:1219	arg1	administration					1183:1196	Oral administration	1178:1196	Oral administration of MG, ML and coumarin	1178:1219	Oral administration of MG, ML and coumarin inhibited paw edema induced by carrageenan (400 mg/kg, 55% inhibition; 400 mg/kg, 57% inhibition; 75 mg/kg, 38% inhibition; p < 0.05, respectively).
30415057	17	67	theme	M.	2132:2133	arg1	glomerata					2135:2143	M. glomerata	2132:2143	M. glomerata	2132:2143	CONCLUSION The variation of the results of the anti-inflammatory activity found in M. glomerata and M. laevigata demonstrates that these two species should not be used interchangeably.
30415057	10	68	theme	coumarin	726:733	arg1	doses					699:703	MATERIAL AND METHODS Different doses	668:703	MATERIAL AND METHODS Different doses of both extracts and coumarin	668:733	MATERIAL AND METHODS Different doses of both extracts and coumarin were given to rats in different experimental models to assess the anti-inflammatory activity between these two species.
30415057	14	69	dep	inhibited	1405:1413	arg1	400 mg/kg					1452:1460	400 mg/kg	1452:1460	400 mg/kg	1452:1460	MG, ML and coumarin treatment also inhibited the edema induced by compound 48/80 (400 mg/kg, 56% inhibition; 400 mg/kg, 69% inhibition; 75 mg/kg, 40% inhibition; p < 0.05, respectively).
30415057	17	70	used	used	2212:2215	arg2	species					2190:2196	these two species	2180:2196	these two species	2180:2196	CONCLUSION The variation of the results of the anti-inflammatory activity found in M. glomerata and M. laevigata demonstrates that these two species should not be used interchangeably.
30415057	11	71	theme	High	1007:1010	arg1	Chromatography					1030:1043	Ultra High Efficiency Liquid Chromatography	1001:1043	Ultra High Efficiency Liquid Chromatography coupled to Mass Spectrometry (UHPLC-MS)	1001:1083	For this, the animals were submitted to paw edema, pleurisy and degranulation of peritoneal mast cell and the extracts were also characterized by Ultra High Efficiency Liquid Chromatography coupled to Mass Spectrometry (UHPLC-MS).
30415057	12	72	dep	RESULTS	1086:1092	arg1	showed					1121:1126	showed	1121:1126	showed that ML presents ten times more coumarin than MG	1121:1175	RESULTS The chromatographic method showed that ML presents ten times more coumarin than MG.
30415057	15	73	theme	peritoneal	1668:1677	arg1	cells					1684:1688	Wistar rat peritoneal mast cells	1657:1688	Wistar rat peritoneal mast cells induced by compound 48/80	1657:1714	MG, ML and coumarin did not prevent mast cell degranulation and the consequent histamine release in Wistar rat peritoneal mast cells induced by compound 48/80.
30415057	16	74	dep	migration	1828:1836	arg1	%					1860:1860	200 and 400 mg/kg, 23% and 30% inhibition	1839:1879	%	1860:1860	MG did not inhibit cell infiltration in pleurisy nor the highest dose tested, while ML decreased the leukocyte migration (200 and 400 mg/kg, 23% and 30% inhibition; p < 0.001, respectively) and, to a lesser extent, coumarin also reduced cell infiltration (10, 50 and 75 mg/kg; 15%, 16% and 17% inhibition; p < 0.001, respectively).
30415057	16	74	dep	migration	1828:1836	arg1	inhibition					1870:1879	200 and 400 mg/kg, 23% and 30% inhibition	1839:1879	inhibition	1870:1879	MG did not inhibit cell infiltration in pleurisy nor the highest dose tested, while ML decreased the leukocyte migration (200 and 400 mg/kg, 23% and 30% inhibition; p < 0.001, respectively) and, to a lesser extent, coumarin also reduced cell infiltration (10, 50 and 75 mg/kg; 15%, 16% and 17% inhibition; p < 0.001, respectively).
30415057	14	75	theme	40	1516:1517	arg1	%					1518:1518	%	1518:1518	%	1518:1518	MG, ML and coumarin treatment also inhibited the edema induced by compound 48/80 (400 mg/kg, 56% inhibition; 400 mg/kg, 69% inhibition; 75 mg/kg, 40% inhibition; p < 0.05, respectively).
30415057	16	76	theme	%	1868:1868	arg1	inhibition					1870:1879	200 and 400 mg/kg, 23% and 30% inhibition	1839:1879	inhibition	1870:1879	MG did not inhibit cell infiltration in pleurisy nor the highest dose tested, while ML decreased the leukocyte migration (200 and 400 mg/kg, 23% and 30% inhibition; p < 0.001, respectively) and, to a lesser extent, coumarin also reduced cell infiltration (10, 50 and 75 mg/kg; 15%, 16% and 17% inhibition; p < 0.001, respectively).
30415057	18	77	theme	therapeutic	2383:2393	arg1	effect					2395:2400	this therapeutic effect	2378:2400	this therapeutic effect in the extracts	2378:2416	Coumarin, as already proven, has anti-inflammatory action however, we have suggested that it probably is not the only component responsible for this therapeutic effect in the extracts.
30415057	13	78	theme	%	1278:1278	arg1	inhibition					1280:1289	55% inhibition	1276:1289	55% inhibition	1276:1289	Oral administration of MG, ML and coumarin inhibited paw edema induced by carrageenan (400 mg/kg, 55% inhibition; 400 mg/kg, 57% inhibition; 75 mg/kg, 38% inhibition; p < 0.05, respectively).
30415057	0	79	theme	Mikania	75:81	arg1	glomerata					83:91	Mikania glomerata	75:91	Mikania glomerata	75:91	Pharmacological study of anti-inflammatory activity of aqueous extracts of Mikania glomerata (Spreng.)
30415057	10	80	theme	different	757:765	arg1	models					780:785	different experimental models	757:785	different experimental models to assess the anti-inflammatory activity between these two species	757:852	MATERIAL AND METHODS Different doses of both extracts and coumarin were given to rats in different experimental models to assess the anti-inflammatory activity between these two species.
30415057	1	81	dep	and	103:105	arg1	laevigata					115:123	laevigata	115:123	laevigata	115:123	and Mikania laevigata (Sch.
30415057	16	82	theme	17	2007:2008	arg1	%					2009:2009	%	2009:2009	%	2009:2009	MG did not inhibit cell infiltration in pleurisy nor the highest dose tested, while ML decreased the leukocyte migration (200 and 400 mg/kg, 23% and 30% inhibition; p < 0.001, respectively) and, to a lesser extent, coumarin also reduced cell infiltration (10, 50 and 75 mg/kg; 15%, 16% and 17% inhibition; p < 0.001, respectively).
30415057	11	83	theme	mast	947:950	arg1	cell					952:955	peritoneal mast cell	936:955	peritoneal mast cell	936:955	For this, the animals were submitted to paw edema, pleurisy and degranulation of peritoneal mast cell and the extracts were also characterized by Ultra High Efficiency Liquid Chromatography coupled to Mass Spectrometry (UHPLC-MS).
30415057	7	84	from	medicinal	351:359	arg1	similar					303:309	similar	303:309	similar	303:309	ex Baker (ML), popularly known as guaco, are medicinal plants similar in morphology, chemical composition and medicinal uses.
30415057	15	85	theme	cell	1598:1601	arg1	degranulation					1603:1615	mast cell degranulation	1593:1615	mast cell degranulation	1593:1615	MG, ML and coumarin did not prevent mast cell degranulation and the consequent histamine release in Wistar rat peritoneal mast cells induced by compound 48/80.
30415057	9	86	theme	study	521:525	arg1	aim					509:511	the aim	505:511	the aim of this study	505:525	AIM Thus, the aim of this study is to investigate if the aqueous extract of MG and ML present similar anti-inflammatory activity to the point of being used interchangeably.
30415057	7	87	theme	ex	241:242	arg1	plants					296:301	medicinal plants	286:301	medicinal plants similar in morphology, chemical composition and medicinal uses	286:364	ex Baker (ML), popularly known as guaco, are medicinal plants similar in morphology, chemical composition and medicinal uses.
30415057	7	87	theme	ex	241:242	arg1	ML					251:252	ML	251:252	ML	251:252	ex Baker (ML), popularly known as guaco, are medicinal plants similar in morphology, chemical composition and medicinal uses.
30415057	7	87	theme	ex	241:242	arg1	Baker					244:248	ex Baker	241:248	ex Baker (ML)	241:253	ex Baker (ML), popularly known as guaco, are medicinal plants similar in morphology, chemical composition and medicinal uses.
30415057	14	88	theme	coumarin	1381:1388	arg1	treatment					1390:1398	coumarin treatment	1381:1398	coumarin treatment	1381:1398	MG, ML and coumarin treatment also inhibited the edema induced by compound 48/80 (400 mg/kg, 56% inhibition; 400 mg/kg, 69% inhibition; 75 mg/kg, 40% inhibition; p < 0.05, respectively).
30415057	1	89	dep	laevigata	115:123	arg1	Sch					126:128	Sch	126:128	Sch	126:128	and Mikania laevigata (Sch.
30415057	10	90	theme	Different	689:697	arg1	doses					699:703	MATERIAL AND METHODS Different doses	668:703	MATERIAL AND METHODS Different doses of both extracts and coumarin	668:733	MATERIAL AND METHODS Different doses of both extracts and coumarin were given to rats in different experimental models to assess the anti-inflammatory activity between these two species.
30415057	14	91	theme	56	1463:1464	arg1	%					1465:1465	%	1465:1465	%	1465:1465	MG, ML and coumarin treatment also inhibited the edema induced by compound 48/80 (400 mg/kg, 56% inhibition; 400 mg/kg, 69% inhibition; 75 mg/kg, 40% inhibition; p < 0.05, respectively).
30415057	8	92	used	used	390:393	arg2	species					372:378	Both species	367:378	Both species	367:378	Both species are often used and sold without distinction; however, it is believed that their chemical composition is different.
30415057	0	93	theme	anti-inflammatory	25:41	arg1	activity					43:50	anti-inflammatory activity	25:50	anti-inflammatory activity of aqueous extracts of Mikania glomerata	25:91	Pharmacological study of anti-inflammatory activity of aqueous extracts of Mikania glomerata (Spreng.)
30415057	13	94	theme	MG	1201:1202	arg1	administration					1183:1196	Oral administration	1178:1196	Oral administration of MG, ML and coumarin	1178:1219	Oral administration of MG, ML and coumarin inhibited paw edema induced by carrageenan (400 mg/kg, 55% inhibition; 400 mg/kg, 57% inhibition; 75 mg/kg, 38% inhibition; p < 0.05, respectively).
30415057	16	95	from	infiltration	1741:1752	arg1	pleurisy					1757:1764	pleurisy	1757:1764	pleurisy	1757:1764	MG did not inhibit cell infiltration in pleurisy nor the highest dose tested, while ML decreased the leukocyte migration (200 and 400 mg/kg, 23% and 30% inhibition; p < 0.001, respectively) and, to a lesser extent, coumarin also reduced cell infiltration (10, 50 and 75 mg/kg; 15%, 16% and 17% inhibition; p < 0.001, respectively).
30415057	10	96	theme	extracts	713:720	arg1	doses					699:703	MATERIAL AND METHODS Different doses	668:703	MATERIAL AND METHODS Different doses of both extracts and coumarin	668:733	MATERIAL AND METHODS Different doses of both extracts and coumarin were given to rats in different experimental models to assess the anti-inflammatory activity between these two species.
30415057	9	97	theme	aqueous	552:558	arg1	extract					560:566	the aqueous extract	548:566	the aqueous extract of MG and ML	548:579	AIM Thus, the aim of this study is to investigate if the aqueous extract of MG and ML present similar anti-inflammatory activity to the point of being used interchangeably.
30415057	16	98	theme	leukocyte	1818:1826	arg1	migration					1828:1836	the leukocyte migration	1814:1836	the leukocyte migration (200 and 400 mg/kg, 23% and 30% inhibition; p < 0.001, respectively)	1814:1905	MG did not inhibit cell infiltration in pleurisy nor the highest dose tested, while ML decreased the leukocyte migration (200 and 400 mg/kg, 23% and 30% inhibition; p < 0.001, respectively) and, to a lesser extent, coumarin also reduced cell infiltration (10, 50 and 75 mg/kg; 15%, 16% and 17% inhibition; p < 0.001, respectively).
30415057	13	99	theme	ML	1205:1206	arg1	administration					1183:1196	Oral administration	1178:1196	Oral administration of MG, ML and coumarin	1178:1219	Oral administration of MG, ML and coumarin inhibited paw edema induced by carrageenan (400 mg/kg, 55% inhibition; 400 mg/kg, 57% inhibition; 75 mg/kg, 38% inhibition; p < 0.05, respectively).
30415057	18	100	contain	has	2263:2265	arg1	Coumarin					2234:2241	Coumarin	2234:2241	Coumarin	2234:2241	Coumarin, as already proven, has anti-inflammatory action however, we have suggested that it probably is not the only component responsible for this therapeutic effect in the extracts.
30415057	18	100	contain	has	2263:2265	arg2	action					2285:2290	anti-inflammatory action	2267:2290	anti-inflammatory action	2267:2290	Coumarin, as already proven, has anti-inflammatory action however, we have suggested that it probably is not the only component responsible for this therapeutic effect in the extracts.
30415057	0	101	theme	extracts	63:70	arg1	activity					43:50	anti-inflammatory activity	25:50	anti-inflammatory activity of aqueous extracts of Mikania glomerata	25:91	Pharmacological study of anti-inflammatory activity of aqueous extracts of Mikania glomerata (Spreng.)
30415057	16	102	theme	lesser	1917:1922	arg1	extent					1924:1929	a lesser extent	1915:1929	a lesser extent	1915:1929	MG did not inhibit cell infiltration in pleurisy nor the highest dose tested, while ML decreased the leukocyte migration (200 and 400 mg/kg, 23% and 30% inhibition; p < 0.001, respectively) and, to a lesser extent, coumarin also reduced cell infiltration (10, 50 and 75 mg/kg; 15%, 16% and 17% inhibition; p < 0.001, respectively).
30415057	11	103	theme	Liquid	1023:1028	arg1	Chromatography					1030:1043	Ultra High Efficiency Liquid Chromatography	1001:1043	Ultra High Efficiency Liquid Chromatography coupled to Mass Spectrometry (UHPLC-MS)	1001:1083	For this, the animals were submitted to paw edema, pleurisy and degranulation of peritoneal mast cell and the extracts were also characterized by Ultra High Efficiency Liquid Chromatography coupled to Mass Spectrometry (UHPLC-MS).
30415057	12	104	theme	coumarin	1160:1167	arg1	times					1149:1153	ten times	1145:1153	ten times more coumarin than MG	1145:1175	RESULTS The chromatographic method showed that ML presents ten times more coumarin than MG.
30415057	17	105	theme	anti-inflammatory	2096:2112	arg1	activity					2114:2121	the anti-inflammatory activity	2092:2121	the anti-inflammatory activity found in M. glomerata and M. laevigata	2092:2160	CONCLUSION The variation of the results of the anti-inflammatory activity found in M. glomerata and M. laevigata demonstrates that these two species should not be used interchangeably.
30415057	15	106	from	release	1646:1652	arg1	cells					1684:1688	Wistar rat peritoneal mast cells	1657:1688	Wistar rat peritoneal mast cells induced by compound 48/80	1657:1714	MG, ML and coumarin did not prevent mast cell degranulation and the consequent histamine release in Wistar rat peritoneal mast cells induced by compound 48/80.
30415057	7	107	from	composition	335:345	arg1	similar					303:309	similar	303:309	similar	303:309	ex Baker (ML), popularly known as guaco, are medicinal plants similar in morphology, chemical composition and medicinal uses.
30415057	13	108	theme	%	1331:1331	arg1	inhibition					1333:1342	38% inhibition	1329:1342	38% inhibition	1329:1342	Oral administration of MG, ML and coumarin inhibited paw edema induced by carrageenan (400 mg/kg, 55% inhibition; 400 mg/kg, 57% inhibition; 75 mg/kg, 38% inhibition; p < 0.05, respectively).
30415057	11	109	theme	Mass	1056:1059	arg1	Spectrometry					1061:1072	Mass Spectrometry	1056:1072	Mass Spectrometry (UHPLC-MS)	1056:1083	For this, the animals were submitted to paw edema, pleurisy and degranulation of peritoneal mast cell and the extracts were also characterized by Ultra High Efficiency Liquid Chromatography coupled to Mass Spectrometry (UHPLC-MS).
30415057	11	109	theme	Mass	1056:1059	arg1	UHPLC-MS					1075:1082	UHPLC-MS	1075:1082	UHPLC-MS	1075:1082	For this, the animals were submitted to paw edema, pleurisy and degranulation of peritoneal mast cell and the extracts were also characterized by Ultra High Efficiency Liquid Chromatography coupled to Mass Spectrometry (UHPLC-MS).
30415057	15	110	theme	rat	1664:1666	arg1	cells					1684:1688	Wistar rat peritoneal mast cells	1657:1688	Wistar rat peritoneal mast cells induced by compound 48/80	1657:1714	MG, ML and coumarin did not prevent mast cell degranulation and the consequent histamine release in Wistar rat peritoneal mast cells induced by compound 48/80.
30415057	13	111	theme	%	1305:1305	arg1	inhibition					1307:1316	57% inhibition	1303:1316	57% inhibition	1303:1316	Oral administration of MG, ML and coumarin inhibited paw edema induced by carrageenan (400 mg/kg, 55% inhibition; 400 mg/kg, 57% inhibition; 75 mg/kg, 38% inhibition; p < 0.05, respectively).
29645025	6	0	theme	adipose	1173:1179	arg1	tissue					1181:1186	adipose tissue	1173:1186	adipose tissue	1173:1186	Besides these, insulin sensitivity and the composition of fatty acid phospholipids of adipose tissue were measured.
29645025	1	1	theme	dyslipemic	451:460	arg1	rats					480:483	dyslipemic insulin-resistant rats	451:483	dyslipemic insulin-resistant rats	451:483	This work aims to assess the possible beneficial effects of dietary fish oil (FO) on the pre-existing adipose tissue dysfunction through the improvement or reversion of the mechanisms underlying oxidative stress and pro-inflammatory cytokines in dyslipemic insulin-resistant rats.
29645025	1	2	theme	dietary	265:271	arg1	FO					283:284	FO	283:284	FO	283:284	This work aims to assess the possible beneficial effects of dietary fish oil (FO) on the pre-existing adipose tissue dysfunction through the improvement or reversion of the mechanisms underlying oxidative stress and pro-inflammatory cytokines in dyslipemic insulin-resistant rats.
29645025	1	2	theme	dietary	265:271	arg1	oil					278:280	dietary fish oil	265:280	dietary fish oil (FO)	265:285	This work aims to assess the possible beneficial effects of dietary fish oil (FO) on the pre-existing adipose tissue dysfunction through the improvement or reversion of the mechanisms underlying oxidative stress and pro-inflammatory cytokines in dyslipemic insulin-resistant rats.
29645025	7	3	theme	antioxidant	1292:1302	arg1	content					1342:1348	ROS content	1338:1348	ROS content	1338:1348	Compared with the SRD the SRD + FO fed group showed a decrease of fat pad weight and the antioxidant and oxidant enzyme activities and ROS content returned to control values along with normal plasma TNF-α and IL-6 levels.
29645025	7	3	theme	antioxidant	1292:1302	arg1	decrease					1257:1264	a decrease	1255:1264	a decrease of fat pad weight and the antioxidant and oxidant enzyme activities	1255:1332	Compared with the SRD the SRD + FO fed group showed a decrease of fat pad weight and the antioxidant and oxidant enzyme activities and ROS content returned to control values along with normal plasma TNF-α and IL-6 levels.
29645025	8	4	theme	protein	1494:1500	arg1	increase					1482:1489	the increase	1478:1489	the increase of protein and expression of UCP2	1478:1523	FO normalized both the decrease of PPARγ protein and the increase of protein and expression of UCP2.
29645025	8	4	theme	protein	1494:1500	arg1	decrease					1448:1455	the decrease	1444:1455	the decrease of PPARγ protein	1444:1472	FO normalized both the decrease of PPARγ protein and the increase of protein and expression of UCP2.
29645025	6	5	theme	phospholipids	1156:1168	arg1	sensitivity					1110:1120	insulin sensitivity	1102:1120	insulin sensitivity	1102:1120	Besides these, insulin sensitivity and the composition of fatty acid phospholipids of adipose tissue were measured.
29645025	6	5	theme	phospholipids	1156:1168	arg1	composition					1130:1140	the composition	1126:1140	the composition of fatty acid phospholipids of adipose tissue	1126:1186	Besides these, insulin sensitivity and the composition of fatty acid phospholipids of adipose tissue were measured.
29645025	1	6	theme	oil	278:280	arg1	effects					254:260	the possible beneficial effects	230:260	the possible beneficial effects of dietary fish oil (FO) on the pre-existing adipose tissue dysfunction	230:332	This work aims to assess the possible beneficial effects of dietary fish oil (FO) on the pre-existing adipose tissue dysfunction through the improvement or reversion of the mechanisms underlying oxidative stress and pro-inflammatory cytokines in dyslipemic insulin-resistant rats.
29645025	6	7	theme	fatty	1145:1149	arg1	phospholipids					1156:1168	fatty acid phospholipids	1145:1168	fatty acid phospholipids of adipose tissue	1145:1186	Besides these, insulin sensitivity and the composition of fatty acid phospholipids of adipose tissue were measured.
29645025	7	8	dep	showed	1248:1253	arg1	returned					1350:1357	returned	1350:1357	showed a decrease of fat pad weight and the antioxidant and oxidant enzyme activities and ROS content returned to control values along with normal plasma TNF-α and IL-6 levels	1248:1422	Compared with the SRD the SRD + FO fed group showed a decrease of fat pad weight and the antioxidant and oxidant enzyme activities and ROS content returned to control values along with normal plasma TNF-α and IL-6 levels.
29645025	7	9	theme	enzyme	1316:1321	arg1	activities					1323:1332	the antioxidant and oxidant enzyme activities	1288:1332	activities	1323:1332	Compared with the SRD the SRD + FO fed group showed a decrease of fat pad weight and the antioxidant and oxidant enzyme activities and ROS content returned to control values along with normal plasma TNF-α and IL-6 levels.
29645025	7	10	theme	pad	1273:1275	arg1	weight					1277:1282	fat pad weight	1269:1282	fat pad weight	1269:1282	Compared with the SRD the SRD + FO fed group showed a decrease of fat pad weight and the antioxidant and oxidant enzyme activities and ROS content returned to control values along with normal plasma TNF-α and IL-6 levels.
29645025	5	11	theme	antioxidant	786:796	arg1	activities					817:826	antioxidant and oxidant enzyme activities	786:826	antioxidant and oxidant enzyme activities	786:826	In an epididymal fat pad, we analyzed antioxidant and oxidant enzyme activities, ROS content, glutathione redox state, the protein level of peroxisome proliferator-activated receptor gamma (PPARγ) and the expression and protein levels of uncoupling protein 2 (UCP2) as well as oxidative stress biomarkers and TNF-α and IL-6 plasma levels.
29645025	1	12	theme	mechanisms	378:387	arg1	reversion					361:369	reversion	361:369	reversion	361:369	This work aims to assess the possible beneficial effects of dietary fish oil (FO) on the pre-existing adipose tissue dysfunction through the improvement or reversion of the mechanisms underlying oxidative stress and pro-inflammatory cytokines in dyslipemic insulin-resistant rats.
29645025	1	12	theme	mechanisms	378:387	arg1	improvement					346:356	improvement	346:356	improvement	346:356	This work aims to assess the possible beneficial effects of dietary fish oil (FO) on the pre-existing adipose tissue dysfunction through the improvement or reversion of the mechanisms underlying oxidative stress and pro-inflammatory cytokines in dyslipemic insulin-resistant rats.
29645025	5	13	theme	epididymal	754:763	arg1	pad					769:771	an epididymal fat pad	751:771	an epididymal fat pad	751:771	In an epididymal fat pad, we analyzed antioxidant and oxidant enzyme activities, ROS content, glutathione redox state, the protein level of peroxisome proliferator-activated receptor gamma (PPARγ) and the expression and protein levels of uncoupling protein 2 (UCP2) as well as oxidative stress biomarkers and TNF-α and IL-6 plasma levels.
29645025	9	14	theme	n-3/n-6	1556:1562	arg1	ratio					1575:1579	the n-3/n-6 fatty acid ratio	1552:1579	the n-3/n-6 fatty acid ratio in the adipose tissue phospholipids and normalized dyslipidemia and insulin resistance	1552:1666	Furthermore, FO increased the n-3/n-6 fatty acid ratio in the adipose tissue phospholipids and normalized dyslipidemia and insulin resistance.
29645025	0	15	theme	peroxisome	134:143	arg1	receptor					168:175	peroxisome proliferator-activated receptor γ	134:177	peroxisome proliferator-activated receptor γ	134:177	Dietary fish oil ameliorates adipose tissue dysfunction in insulin-resistant rats fed a sucrose-rich diet improving oxidative stress, peroxisome proliferator-activated receptor γ and uncoupling protein 2.
29645025	5	16	theme	uncoupling	986:995	arg1	UCP2					1008:1011	UCP2	1008:1011	UCP2	1008:1011	In an epididymal fat pad, we analyzed antioxidant and oxidant enzyme activities, ROS content, glutathione redox state, the protein level of peroxisome proliferator-activated receptor gamma (PPARγ) and the expression and protein levels of uncoupling protein 2 (UCP2) as well as oxidative stress biomarkers and TNF-α and IL-6 plasma levels.
29645025	5	16	theme	uncoupling	986:995	arg1	protein					997:1003	uncoupling protein 2	986:1005	uncoupling protein 2 (UCP2) as well as oxidative stress biomarkers and TNF-α and IL-6 plasma levels	986:1084	In an epididymal fat pad, we analyzed antioxidant and oxidant enzyme activities, ROS content, glutathione redox state, the protein level of peroxisome proliferator-activated receptor gamma (PPARγ) and the expression and protein levels of uncoupling protein 2 (UCP2) as well as oxidative stress biomarkers and TNF-α and IL-6 plasma levels.
29645025	10	17	from	resistance	1803:1812	arg1	model					1829:1833	this animal model	1817:1833	this animal model	1817:1833	Finally, these findings reinforce the view that dietary FO may exert a beneficial effect in ameliorating the dyslipidemia and insulin resistance in this animal model.
29645025	9	18	theme	acid	1570:1573	arg1	ratio					1575:1579	the n-3/n-6 fatty acid ratio	1552:1579	the n-3/n-6 fatty acid ratio in the adipose tissue phospholipids and normalized dyslipidemia and insulin resistance	1552:1666	Furthermore, FO increased the n-3/n-6 fatty acid ratio in the adipose tissue phospholipids and normalized dyslipidemia and insulin resistance.
29645025	10	19	dep	dyslipidemia	1778:1789	arg1	the					1774:1776	the	1774:1776	the	1774:1776	Finally, these findings reinforce the view that dietary FO may exert a beneficial effect in ameliorating the dyslipidemia and insulin resistance in this animal model.
29645025	1	20	from	effects	254:260	arg1	dysfunction					322:332	the pre-existing adipose tissue dysfunction	290:332	the pre-existing adipose tissue dysfunction	290:332	This work aims to assess the possible beneficial effects of dietary fish oil (FO) on the pre-existing adipose tissue dysfunction through the improvement or reversion of the mechanisms underlying oxidative stress and pro-inflammatory cytokines in dyslipemic insulin-resistant rats.
29645025	1	21	theme	pro-inflammatory	421:436	arg1	cytokines					438:446	pro-inflammatory cytokines	421:446	pro-inflammatory cytokines	421:446	This work aims to assess the possible beneficial effects of dietary fish oil (FO) on the pre-existing adipose tissue dysfunction through the improvement or reversion of the mechanisms underlying oxidative stress and pro-inflammatory cytokines in dyslipemic insulin-resistant rats.
29645025	5	22	theme	ROS	829:831	arg1	content					833:839	ROS content	829:839	ROS content	829:839	In an epididymal fat pad, we analyzed antioxidant and oxidant enzyme activities, ROS content, glutathione redox state, the protein level of peroxisome proliferator-activated receptor gamma (PPARγ) and the expression and protein levels of uncoupling protein 2 (UCP2) as well as oxidative stress biomarkers and TNF-α and IL-6 plasma levels.
29645025	7	23	theme	FO	1235:1236	arg1	group					1242:1246	the SRD + FO fed group	1225:1246	the SRD + FO fed group	1225:1246	Compared with the SRD the SRD + FO fed group showed a decrease of fat pad weight and the antioxidant and oxidant enzyme activities and ROS content returned to control values along with normal plasma TNF-α and IL-6 levels.
29645025	9	24	theme	adipose	1588:1594	arg1	tissue					1596:1601	the adipose tissue phospholipids and normalized dyslipidemia and insulin resistance	1584:1666	tissue	1596:1601	Furthermore, FO increased the n-3/n-6 fatty acid ratio in the adipose tissue phospholipids and normalized dyslipidemia and insulin resistance.
29645025	7	25	theme	SRD	1229:1231	arg1	group					1242:1246	the SRD + FO fed group	1225:1246	the SRD + FO fed group	1225:1246	Compared with the SRD the SRD + FO fed group showed a decrease of fat pad weight and the antioxidant and oxidant enzyme activities and ROS content returned to control values along with normal plasma TNF-α and IL-6 levels.
29645025	0	26	theme	insulin-resistant	59:75	arg1	rats					77:80	insulin-resistant rats	59:80	insulin-resistant rats fed a sucrose-rich diet improving oxidative stress, peroxisome proliferator-activated receptor γ and uncoupling protein 2	59:202	Dietary fish oil ameliorates adipose tissue dysfunction in insulin-resistant rats fed a sucrose-rich diet improving oxidative stress, peroxisome proliferator-activated receptor γ and uncoupling protein 2.
29645025	5	27	theme	oxidant	802:808	arg1	activities					817:826	antioxidant and oxidant enzyme activities	786:826	antioxidant and oxidant enzyme activities	786:826	In an epididymal fat pad, we analyzed antioxidant and oxidant enzyme activities, ROS content, glutathione redox state, the protein level of peroxisome proliferator-activated receptor gamma (PPARγ) and the expression and protein levels of uncoupling protein 2 (UCP2) as well as oxidative stress biomarkers and TNF-α and IL-6 plasma levels.
29645025	9	28	from	ratio	1575:1579	arg1	dyslipidemia					1632:1643	dyslipidemia	1632:1643	dyslipidemia	1632:1643	Furthermore, FO increased the n-3/n-6 fatty acid ratio in the adipose tissue phospholipids and normalized dyslipidemia and insulin resistance.
29645025	9	28	from	ratio	1575:1579	arg1	tissue					1596:1601	the adipose tissue phospholipids and normalized dyslipidemia and insulin resistance	1584:1666	tissue	1596:1601	Furthermore, FO increased the n-3/n-6 fatty acid ratio in the adipose tissue phospholipids and normalized dyslipidemia and insulin resistance.
29645025	9	29	dep	tissue	1596:1601	arg1	phospholipids					1603:1615	phospholipids	1603:1615	phospholipids	1603:1615	Furthermore, FO increased the n-3/n-6 fatty acid ratio in the adipose tissue phospholipids and normalized dyslipidemia and insulin resistance.
29645025	9	30	theme	normalized	1621:1630	arg1	dyslipidemia					1632:1643	dyslipidemia	1632:1643	dyslipidemia	1632:1643	Furthermore, FO increased the n-3/n-6 fatty acid ratio in the adipose tissue phospholipids and normalized dyslipidemia and insulin resistance.
29645025	4	31	theme	reference	694:702	arg1	group					704:708	A reference group	692:708	A reference group	692:708	A reference group consumed a control diet all the time.
29645025	0	32	theme	sucrose-rich	88:99	arg1	diet					101:104	a sucrose-rich diet	86:104	a sucrose-rich diet improving oxidative stress, peroxisome proliferator-activated receptor γ and uncoupling protein 2	86:202	Dietary fish oil ameliorates adipose tissue dysfunction in insulin-resistant rats fed a sucrose-rich diet improving oxidative stress, peroxisome proliferator-activated receptor γ and uncoupling protein 2.
29645025	5	33	theme	protein	871:877	arg1	level					879:883	the protein level	867:883	the protein level of peroxisome proliferator-activated receptor gamma (PPARγ)	867:943	In an epididymal fat pad, we analyzed antioxidant and oxidant enzyme activities, ROS content, glutathione redox state, the protein level of peroxisome proliferator-activated receptor gamma (PPARγ) and the expression and protein levels of uncoupling protein 2 (UCP2) as well as oxidative stress biomarkers and TNF-α and IL-6 plasma levels.
29645025	0	34	from	dysfunction	44:54	arg1	rats					77:80	insulin-resistant rats	59:80	insulin-resistant rats fed a sucrose-rich diet improving oxidative stress, peroxisome proliferator-activated receptor γ and uncoupling protein 2	59:202	Dietary fish oil ameliorates adipose tissue dysfunction in insulin-resistant rats fed a sucrose-rich diet improving oxidative stress, peroxisome proliferator-activated receptor γ and uncoupling protein 2.
29645025	0	35	theme	Dietary	0:6	arg1	oil					13:15	Dietary fish oil	0:15	Dietary fish oil	0:15	Dietary fish oil ameliorates adipose tissue dysfunction in insulin-resistant rats fed a sucrose-rich diet improving oxidative stress, peroxisome proliferator-activated receptor γ and uncoupling protein 2.
29645025	5	36	theme	redox	854:858	arg1	state					860:864	glutathione redox state	842:864	glutathione redox state	842:864	In an epididymal fat pad, we analyzed antioxidant and oxidant enzyme activities, ROS content, glutathione redox state, the protein level of peroxisome proliferator-activated receptor gamma (PPARγ) and the expression and protein levels of uncoupling protein 2 (UCP2) as well as oxidative stress biomarkers and TNF-α and IL-6 plasma levels.
29645025	10	37	theme	dietary	1717:1723	arg1	FO					1725:1726	dietary FO	1717:1726	dietary FO	1717:1726	Finally, these findings reinforce the view that dietary FO may exert a beneficial effect in ameliorating the dyslipidemia and insulin resistance in this animal model.
29645025	1	38	dep	improvement	346:356	arg1	the					342:344	the	342:344	the	342:344	This work aims to assess the possible beneficial effects of dietary fish oil (FO) on the pre-existing adipose tissue dysfunction through the improvement or reversion of the mechanisms underlying oxidative stress and pro-inflammatory cytokines in dyslipemic insulin-resistant rats.
29645025	5	39	theme	plasma	1072:1077	arg1	levels					1079:1084	TNF-α and IL-6 plasma levels	1057:1084	levels	1079:1084	In an epididymal fat pad, we analyzed antioxidant and oxidant enzyme activities, ROS content, glutathione redox state, the protein level of peroxisome proliferator-activated receptor gamma (PPARγ) and the expression and protein levels of uncoupling protein 2 (UCP2) as well as oxidative stress biomarkers and TNF-α and IL-6 plasma levels.
29645025	3	40	theme	other	628:632	arg1	half					634:637	the other half	624:637	the other half	624:637	After that half of the animals continued with the SRD until month 8 while in the other half corn oil was replaced by FO for 2 months (SRD + FO).
29645025	0	41	theme	adipose	29:35	arg1	dysfunction					44:54	adipose tissue dysfunction	29:54	adipose tissue dysfunction in insulin-resistant rats fed a sucrose-rich diet improving oxidative stress, peroxisome proliferator-activated receptor γ and uncoupling protein 2	29:202	Dietary fish oil ameliorates adipose tissue dysfunction in insulin-resistant rats fed a sucrose-rich diet improving oxidative stress, peroxisome proliferator-activated receptor γ and uncoupling protein 2.
29645025	5	42	theme	stress	1035:1040	arg1	biomarkers					1042:1051	oxidative stress biomarkers	1025:1051	oxidative stress biomarkers	1025:1051	In an epididymal fat pad, we analyzed antioxidant and oxidant enzyme activities, ROS content, glutathione redox state, the protein level of peroxisome proliferator-activated receptor gamma (PPARγ) and the expression and protein levels of uncoupling protein 2 (UCP2) as well as oxidative stress biomarkers and TNF-α and IL-6 plasma levels.
29645025	7	43	theme	normal	1388:1393	arg1	TNF-α					1402:1406	normal plasma TNF-α and IL-6 levels	1388:1422	TNF-α	1402:1406	Compared with the SRD the SRD + FO fed group showed a decrease of fat pad weight and the antioxidant and oxidant enzyme activities and ROS content returned to control values along with normal plasma TNF-α and IL-6 levels.
29645025	1	44	theme	adipose	307:313	arg1	dysfunction					322:332	the pre-existing adipose tissue dysfunction	290:332	the pre-existing adipose tissue dysfunction	290:332	This work aims to assess the possible beneficial effects of dietary fish oil (FO) on the pre-existing adipose tissue dysfunction through the improvement or reversion of the mechanisms underlying oxidative stress and pro-inflammatory cytokines in dyslipemic insulin-resistant rats.
29645025	4	45	theme	control	721:727	arg1	diet					729:732	a control diet	719:732	a control diet	719:732	A reference group consumed a control diet all the time.
29645025	7	46	theme	control	1362:1368	arg1	values					1370:1375	control values	1362:1375	control values	1362:1375	Compared with the SRD the SRD + FO fed group showed a decrease of fat pad weight and the antioxidant and oxidant enzyme activities and ROS content returned to control values along with normal plasma TNF-α and IL-6 levels.
29645025	5	47	theme	proliferator-activated	899:920	arg1	PPARγ					938:942	PPARγ	938:942	PPARγ	938:942	In an epididymal fat pad, we analyzed antioxidant and oxidant enzyme activities, ROS content, glutathione redox state, the protein level of peroxisome proliferator-activated receptor gamma (PPARγ) and the expression and protein levels of uncoupling protein 2 (UCP2) as well as oxidative stress biomarkers and TNF-α and IL-6 plasma levels.
29645025	5	47	theme	proliferator-activated	899:920	arg1	gamma					931:935	peroxisome proliferator-activated receptor gamma	888:935	peroxisome proliferator-activated receptor gamma (PPARγ)	888:943	In an epididymal fat pad, we analyzed antioxidant and oxidant enzyme activities, ROS content, glutathione redox state, the protein level of peroxisome proliferator-activated receptor gamma (PPARγ) and the expression and protein levels of uncoupling protein 2 (UCP2) as well as oxidative stress biomarkers and TNF-α and IL-6 plasma levels.
29645025	3	48	theme	corn	639:642	arg1	oil					644:646	corn oil	639:646	corn oil	639:646	After that half of the animals continued with the SRD until month 8 while in the other half corn oil was replaced by FO for 2 months (SRD + FO).
29645025	1	49	theme	beneficial	243:252	arg1	effects					254:260	the possible beneficial effects	230:260	the possible beneficial effects of dietary fish oil (FO) on the pre-existing adipose tissue dysfunction	230:332	This work aims to assess the possible beneficial effects of dietary fish oil (FO) on the pre-existing adipose tissue dysfunction through the improvement or reversion of the mechanisms underlying oxidative stress and pro-inflammatory cytokines in dyslipemic insulin-resistant rats.
29645025	5	50	theme	biomarkers	1042:1051	arg1	state					860:864	glutathione redox state	842:864	glutathione redox state	842:864	In an epididymal fat pad, we analyzed antioxidant and oxidant enzyme activities, ROS content, glutathione redox state, the protein level of peroxisome proliferator-activated receptor gamma (PPARγ) and the expression and protein levels of uncoupling protein 2 (UCP2) as well as oxidative stress biomarkers and TNF-α and IL-6 plasma levels.
29645025	5	50	theme	biomarkers	1042:1051	arg1	content					833:839	ROS content	829:839	ROS content	829:839	In an epididymal fat pad, we analyzed antioxidant and oxidant enzyme activities, ROS content, glutathione redox state, the protein level of peroxisome proliferator-activated receptor gamma (PPARγ) and the expression and protein levels of uncoupling protein 2 (UCP2) as well as oxidative stress biomarkers and TNF-α and IL-6 plasma levels.
29645025	5	50	theme	biomarkers	1042:1051	arg1	activities					817:826	antioxidant and oxidant enzyme activities	786:826	antioxidant and oxidant enzyme activities	786:826	In an epididymal fat pad, we analyzed antioxidant and oxidant enzyme activities, ROS content, glutathione redox state, the protein level of peroxisome proliferator-activated receptor gamma (PPARγ) and the expression and protein levels of uncoupling protein 2 (UCP2) as well as oxidative stress biomarkers and TNF-α and IL-6 plasma levels.
29645025	5	50	theme	biomarkers	1042:1051	arg1	level					879:883	the protein level	867:883	the protein level of peroxisome proliferator-activated receptor gamma (PPARγ)	867:943	In an epididymal fat pad, we analyzed antioxidant and oxidant enzyme activities, ROS content, glutathione redox state, the protein level of peroxisome proliferator-activated receptor gamma (PPARγ) and the expression and protein levels of uncoupling protein 2 (UCP2) as well as oxidative stress biomarkers and TNF-α and IL-6 plasma levels.
29645025	5	50	theme	biomarkers	1042:1051	arg1	levels					976:981	the expression and protein levels	949:981	levels	976:981	In an epididymal fat pad, we analyzed antioxidant and oxidant enzyme activities, ROS content, glutathione redox state, the protein level of peroxisome proliferator-activated receptor gamma (PPARγ) and the expression and protein levels of uncoupling protein 2 (UCP2) as well as oxidative stress biomarkers and TNF-α and IL-6 plasma levels.
29645025	5	51	theme	gamma	931:935	arg1	state					860:864	glutathione redox state	842:864	glutathione redox state	842:864	In an epididymal fat pad, we analyzed antioxidant and oxidant enzyme activities, ROS content, glutathione redox state, the protein level of peroxisome proliferator-activated receptor gamma (PPARγ) and the expression and protein levels of uncoupling protein 2 (UCP2) as well as oxidative stress biomarkers and TNF-α and IL-6 plasma levels.
29645025	5	51	theme	gamma	931:935	arg1	content					833:839	ROS content	829:839	ROS content	829:839	In an epididymal fat pad, we analyzed antioxidant and oxidant enzyme activities, ROS content, glutathione redox state, the protein level of peroxisome proliferator-activated receptor gamma (PPARγ) and the expression and protein levels of uncoupling protein 2 (UCP2) as well as oxidative stress biomarkers and TNF-α and IL-6 plasma levels.
29645025	5	51	theme	gamma	931:935	arg1	activities					817:826	antioxidant and oxidant enzyme activities	786:826	antioxidant and oxidant enzyme activities	786:826	In an epididymal fat pad, we analyzed antioxidant and oxidant enzyme activities, ROS content, glutathione redox state, the protein level of peroxisome proliferator-activated receptor gamma (PPARγ) and the expression and protein levels of uncoupling protein 2 (UCP2) as well as oxidative stress biomarkers and TNF-α and IL-6 plasma levels.
29645025	5	51	theme	gamma	931:935	arg1	level					879:883	the protein level	867:883	the protein level of peroxisome proliferator-activated receptor gamma (PPARγ)	867:943	In an epididymal fat pad, we analyzed antioxidant and oxidant enzyme activities, ROS content, glutathione redox state, the protein level of peroxisome proliferator-activated receptor gamma (PPARγ) and the expression and protein levels of uncoupling protein 2 (UCP2) as well as oxidative stress biomarkers and TNF-α and IL-6 plasma levels.
29645025	5	51	theme	gamma	931:935	arg1	levels					976:981	the expression and protein levels	949:981	levels	976:981	In an epididymal fat pad, we analyzed antioxidant and oxidant enzyme activities, ROS content, glutathione redox state, the protein level of peroxisome proliferator-activated receptor gamma (PPARγ) and the expression and protein levels of uncoupling protein 2 (UCP2) as well as oxidative stress biomarkers and TNF-α and IL-6 plasma levels.
29645025	8	52	theme	protein	1466:1472	arg1	increase					1482:1489	the increase	1478:1489	the increase of protein and expression of UCP2	1478:1523	FO normalized both the decrease of PPARγ protein and the increase of protein and expression of UCP2.
29645025	8	52	theme	protein	1466:1472	arg1	decrease					1448:1455	the decrease	1444:1455	the decrease of PPARγ protein	1444:1472	FO normalized both the decrease of PPARγ protein and the increase of protein and expression of UCP2.
29645025	6	53	theme	tissue	1181:1186	arg1	phospholipids					1156:1168	fatty acid phospholipids	1145:1168	fatty acid phospholipids of adipose tissue	1145:1186	Besides these, insulin sensitivity and the composition of fatty acid phospholipids of adipose tissue were measured.
29645025	7	54	theme	ROS	1338:1340	arg1	content					1342:1348	ROS content	1338:1348	ROS content	1338:1348	Compared with the SRD the SRD + FO fed group showed a decrease of fat pad weight and the antioxidant and oxidant enzyme activities and ROS content returned to control values along with normal plasma TNF-α and IL-6 levels.
29645025	1	55	theme	insulin-resistant	462:478	arg1	rats					480:483	dyslipemic insulin-resistant rats	451:483	dyslipemic insulin-resistant rats	451:483	This work aims to assess the possible beneficial effects of dietary fish oil (FO) on the pre-existing adipose tissue dysfunction through the improvement or reversion of the mechanisms underlying oxidative stress and pro-inflammatory cytokines in dyslipemic insulin-resistant rats.
29645025	1	56	theme	fish	273:276	arg1	FO					283:284	FO	283:284	FO	283:284	This work aims to assess the possible beneficial effects of dietary fish oil (FO) on the pre-existing adipose tissue dysfunction through the improvement or reversion of the mechanisms underlying oxidative stress and pro-inflammatory cytokines in dyslipemic insulin-resistant rats.
29645025	1	56	theme	fish	273:276	arg1	oil					278:280	dietary fish oil	265:280	dietary fish oil (FO)	265:285	This work aims to assess the possible beneficial effects of dietary fish oil (FO) on the pre-existing adipose tissue dysfunction through the improvement or reversion of the mechanisms underlying oxidative stress and pro-inflammatory cytokines in dyslipemic insulin-resistant rats.
29645025	10	57	theme	insulin	1795:1801	arg1	resistance					1803:1812	insulin resistance	1795:1812	insulin resistance	1795:1812	Finally, these findings reinforce the view that dietary FO may exert a beneficial effect in ameliorating the dyslipidemia and insulin resistance in this animal model.
29645025	6	58	theme	acid	1151:1154	arg1	phospholipids					1156:1168	fatty acid phospholipids	1145:1168	fatty acid phospholipids of adipose tissue	1145:1186	Besides these, insulin sensitivity and the composition of fatty acid phospholipids of adipose tissue were measured.
29645025	7	59	theme	oxidant	1308:1314	arg1	activities					1323:1332	the antioxidant and oxidant enzyme activities	1288:1332	activities	1323:1332	Compared with the SRD the SRD + FO fed group showed a decrease of fat pad weight and the antioxidant and oxidant enzyme activities and ROS content returned to control values along with normal plasma TNF-α and IL-6 levels.
29645025	7	60	theme	weight	1277:1282	arg1	content					1342:1348	ROS content	1338:1348	ROS content	1338:1348	Compared with the SRD the SRD + FO fed group showed a decrease of fat pad weight and the antioxidant and oxidant enzyme activities and ROS content returned to control values along with normal plasma TNF-α and IL-6 levels.
29645025	7	60	theme	weight	1277:1282	arg1	decrease					1257:1264	a decrease	1255:1264	a decrease of fat pad weight and the antioxidant and oxidant enzyme activities	1255:1332	Compared with the SRD the SRD + FO fed group showed a decrease of fat pad weight and the antioxidant and oxidant enzyme activities and ROS content returned to control values along with normal plasma TNF-α and IL-6 levels.
29645025	10	61	theme	animal	1822:1827	arg1	model					1829:1833	this animal model	1817:1833	this animal model	1817:1833	Finally, these findings reinforce the view that dietary FO may exert a beneficial effect in ameliorating the dyslipidemia and insulin resistance in this animal model.
29645025	7	62	theme	fat	1269:1271	arg1	weight					1277:1282	fat pad weight	1269:1282	fat pad weight	1269:1282	Compared with the SRD the SRD + FO fed group showed a decrease of fat pad weight and the antioxidant and oxidant enzyme activities and ROS content returned to control values along with normal plasma TNF-α and IL-6 levels.
29645025	0	63	theme	proliferator-activated	145:166	arg1	receptor					168:175	peroxisome proliferator-activated receptor γ	134:177	peroxisome proliferator-activated receptor γ	134:177	Dietary fish oil ameliorates adipose tissue dysfunction in insulin-resistant rats fed a sucrose-rich diet improving oxidative stress, peroxisome proliferator-activated receptor γ and uncoupling protein 2.
29645025	5	64	theme	fat	765:767	arg1	pad					769:771	an epididymal fat pad	751:771	an epididymal fat pad	751:771	In an epididymal fat pad, we analyzed antioxidant and oxidant enzyme activities, ROS content, glutathione redox state, the protein level of peroxisome proliferator-activated receptor gamma (PPARγ) and the expression and protein levels of uncoupling protein 2 (UCP2) as well as oxidative stress biomarkers and TNF-α and IL-6 plasma levels.
29645025	10	65	from	dyslipidemia	1778:1789	arg1	model					1829:1833	this animal model	1817:1833	this animal model	1817:1833	Finally, these findings reinforce the view that dietary FO may exert a beneficial effect in ameliorating the dyslipidemia and insulin resistance in this animal model.
29645025	9	66	theme	fatty	1564:1568	arg1	ratio					1575:1579	the n-3/n-6 fatty acid ratio	1552:1579	the n-3/n-6 fatty acid ratio in the adipose tissue phospholipids and normalized dyslipidemia and insulin resistance	1552:1666	Furthermore, FO increased the n-3/n-6 fatty acid ratio in the adipose tissue phospholipids and normalized dyslipidemia and insulin resistance.
29645025	1	67	theme	tissue	315:320	arg1	dysfunction					322:332	the pre-existing adipose tissue dysfunction	290:332	the pre-existing adipose tissue dysfunction	290:332	This work aims to assess the possible beneficial effects of dietary fish oil (FO) on the pre-existing adipose tissue dysfunction through the improvement or reversion of the mechanisms underlying oxidative stress and pro-inflammatory cytokines in dyslipemic insulin-resistant rats.
29645025	5	68	theme	protein	997:1003	arg1	state					860:864	glutathione redox state	842:864	glutathione redox state	842:864	In an epididymal fat pad, we analyzed antioxidant and oxidant enzyme activities, ROS content, glutathione redox state, the protein level of peroxisome proliferator-activated receptor gamma (PPARγ) and the expression and protein levels of uncoupling protein 2 (UCP2) as well as oxidative stress biomarkers and TNF-α and IL-6 plasma levels.
29645025	5	68	theme	protein	997:1003	arg1	content					833:839	ROS content	829:839	ROS content	829:839	In an epididymal fat pad, we analyzed antioxidant and oxidant enzyme activities, ROS content, glutathione redox state, the protein level of peroxisome proliferator-activated receptor gamma (PPARγ) and the expression and protein levels of uncoupling protein 2 (UCP2) as well as oxidative stress biomarkers and TNF-α and IL-6 plasma levels.
29645025	5	68	theme	protein	997:1003	arg1	activities					817:826	antioxidant and oxidant enzyme activities	786:826	antioxidant and oxidant enzyme activities	786:826	In an epididymal fat pad, we analyzed antioxidant and oxidant enzyme activities, ROS content, glutathione redox state, the protein level of peroxisome proliferator-activated receptor gamma (PPARγ) and the expression and protein levels of uncoupling protein 2 (UCP2) as well as oxidative stress biomarkers and TNF-α and IL-6 plasma levels.
29645025	5	68	theme	protein	997:1003	arg1	level					879:883	the protein level	867:883	the protein level of peroxisome proliferator-activated receptor gamma (PPARγ)	867:943	In an epididymal fat pad, we analyzed antioxidant and oxidant enzyme activities, ROS content, glutathione redox state, the protein level of peroxisome proliferator-activated receptor gamma (PPARγ) and the expression and protein levels of uncoupling protein 2 (UCP2) as well as oxidative stress biomarkers and TNF-α and IL-6 plasma levels.
29645025	5	68	theme	protein	997:1003	arg1	levels					976:981	the expression and protein levels	949:981	levels	976:981	In an epididymal fat pad, we analyzed antioxidant and oxidant enzyme activities, ROS content, glutathione redox state, the protein level of peroxisome proliferator-activated receptor gamma (PPARγ) and the expression and protein levels of uncoupling protein 2 (UCP2) as well as oxidative stress biomarkers and TNF-α and IL-6 plasma levels.
29645025	3	69	theme	animals	570:576	arg1	half					558:561	half	558:561	half of the animals	558:576	After that half of the animals continued with the SRD until month 8 while in the other half corn oil was replaced by FO for 2 months (SRD + FO).
29645025	6	70	theme	insulin	1102:1108	arg1	sensitivity					1110:1120	insulin sensitivity	1102:1120	insulin sensitivity	1102:1120	Besides these, insulin sensitivity and the composition of fatty acid phospholipids of adipose tissue were measured.
29645025	0	71	theme	uncoupling	183:192	arg1	protein					194:200	uncoupling protein 2	183:202	uncoupling protein 2	183:202	Dietary fish oil ameliorates adipose tissue dysfunction in insulin-resistant rats fed a sucrose-rich diet improving oxidative stress, peroxisome proliferator-activated receptor γ and uncoupling protein 2.
29645025	5	72	theme	expression	953:962	arg1	levels					976:981	the expression and protein levels	949:981	levels	976:981	In an epididymal fat pad, we analyzed antioxidant and oxidant enzyme activities, ROS content, glutathione redox state, the protein level of peroxisome proliferator-activated receptor gamma (PPARγ) and the expression and protein levels of uncoupling protein 2 (UCP2) as well as oxidative stress biomarkers and TNF-α and IL-6 plasma levels.
29645025	1	73	theme	pre-existing	294:305	arg1	dysfunction					322:332	the pre-existing adipose tissue dysfunction	290:332	the pre-existing adipose tissue dysfunction	290:332	This work aims to assess the possible beneficial effects of dietary fish oil (FO) on the pre-existing adipose tissue dysfunction through the improvement or reversion of the mechanisms underlying oxidative stress and pro-inflammatory cytokines in dyslipemic insulin-resistant rats.
29645025	7	74	theme	fed	1238:1240	arg1	group					1242:1246	the SRD + FO fed group	1225:1246	the SRD + FO fed group	1225:1246	Compared with the SRD the SRD + FO fed group showed a decrease of fat pad weight and the antioxidant and oxidant enzyme activities and ROS content returned to control values along with normal plasma TNF-α and IL-6 levels.
29645025	1	75	from	cytokines	438:446	arg1	rats					480:483	dyslipemic insulin-resistant rats	451:483	dyslipemic insulin-resistant rats	451:483	This work aims to assess the possible beneficial effects of dietary fish oil (FO) on the pre-existing adipose tissue dysfunction through the improvement or reversion of the mechanisms underlying oxidative stress and pro-inflammatory cytokines in dyslipemic insulin-resistant rats.
29645025	5	76	theme	protein	968:974	arg1	levels					976:981	the expression and protein levels	949:981	levels	976:981	In an epididymal fat pad, we analyzed antioxidant and oxidant enzyme activities, ROS content, glutathione redox state, the protein level of peroxisome proliferator-activated receptor gamma (PPARγ) and the expression and protein levels of uncoupling protein 2 (UCP2) as well as oxidative stress biomarkers and TNF-α and IL-6 plasma levels.
29645025	2	77	theme	rich	517:520	arg1	SRD					528:530	SRD	528:530	SRD	528:530	Wistar rats were fed a sucrose rich diet (SRD) for 6 months.
29645025	2	77	theme	rich	517:520	arg1	diet					522:525	a sucrose rich diet	507:525	a sucrose rich diet (SRD)	507:531	Wistar rats were fed a sucrose rich diet (SRD) for 6 months.
29645025	1	78	from	stress	410:415	arg1	rats					480:483	dyslipemic insulin-resistant rats	451:483	dyslipemic insulin-resistant rats	451:483	This work aims to assess the possible beneficial effects of dietary fish oil (FO) on the pre-existing adipose tissue dysfunction through the improvement or reversion of the mechanisms underlying oxidative stress and pro-inflammatory cytokines in dyslipemic insulin-resistant rats.
29645025	5	79	theme	enzyme	810:815	arg1	activities					817:826	antioxidant and oxidant enzyme activities	786:826	antioxidant and oxidant enzyme activities	786:826	In an epididymal fat pad, we analyzed antioxidant and oxidant enzyme activities, ROS content, glutathione redox state, the protein level of peroxisome proliferator-activated receptor gamma (PPARγ) and the expression and protein levels of uncoupling protein 2 (UCP2) as well as oxidative stress biomarkers and TNF-α and IL-6 plasma levels.
29645025	5	80	theme	oxidative	1025:1033	arg1	biomarkers					1042:1051	oxidative stress biomarkers	1025:1051	oxidative stress biomarkers	1025:1051	In an epididymal fat pad, we analyzed antioxidant and oxidant enzyme activities, ROS content, glutathione redox state, the protein level of peroxisome proliferator-activated receptor gamma (PPARγ) and the expression and protein levels of uncoupling protein 2 (UCP2) as well as oxidative stress biomarkers and TNF-α and IL-6 plasma levels.
29645025	7	81	theme	IL-6	1412:1415	arg1	levels					1417:1422	normal plasma TNF-α and IL-6 levels	1388:1422	levels	1417:1422	Compared with the SRD the SRD + FO fed group showed a decrease of fat pad weight and the antioxidant and oxidant enzyme activities and ROS content returned to control values along with normal plasma TNF-α and IL-6 levels.
29645025	0	82	theme	oxidative	116:124	arg1	stress					126:131	oxidative stress	116:131	oxidative stress	116:131	Dietary fish oil ameliorates adipose tissue dysfunction in insulin-resistant rats fed a sucrose-rich diet improving oxidative stress, peroxisome proliferator-activated receptor γ and uncoupling protein 2.
29645025	9	83	theme	insulin	1649:1655	arg1	resistance					1657:1666	insulin resistance	1649:1666	insulin resistance	1649:1666	Furthermore, FO increased the n-3/n-6 fatty acid ratio in the adipose tissue phospholipids and normalized dyslipidemia and insulin resistance.
29645025	0	84	theme	fish	8:11	arg1	oil					13:15	Dietary fish oil	0:15	Dietary fish oil	0:15	Dietary fish oil ameliorates adipose tissue dysfunction in insulin-resistant rats fed a sucrose-rich diet improving oxidative stress, peroxisome proliferator-activated receptor γ and uncoupling protein 2.
29645025	5	85	theme	glutathione	842:852	arg1	state					860:864	glutathione redox state	842:864	glutathione redox state	842:864	In an epididymal fat pad, we analyzed antioxidant and oxidant enzyme activities, ROS content, glutathione redox state, the protein level of peroxisome proliferator-activated receptor gamma (PPARγ) and the expression and protein levels of uncoupling protein 2 (UCP2) as well as oxidative stress biomarkers and TNF-α and IL-6 plasma levels.
29645025	5	86	theme	IL-6	1067:1070	arg1	levels					1079:1084	TNF-α and IL-6 plasma levels	1057:1084	levels	1079:1084	In an epididymal fat pad, we analyzed antioxidant and oxidant enzyme activities, ROS content, glutathione redox state, the protein level of peroxisome proliferator-activated receptor gamma (PPARγ) and the expression and protein levels of uncoupling protein 2 (UCP2) as well as oxidative stress biomarkers and TNF-α and IL-6 plasma levels.
29645025	0	87	theme	tissue	37:42	arg1	dysfunction					44:54	adipose tissue dysfunction	29:54	adipose tissue dysfunction in insulin-resistant rats fed a sucrose-rich diet improving oxidative stress, peroxisome proliferator-activated receptor γ and uncoupling protein 2	29:202	Dietary fish oil ameliorates adipose tissue dysfunction in insulin-resistant rats fed a sucrose-rich diet improving oxidative stress, peroxisome proliferator-activated receptor γ and uncoupling protein 2.
29645025	3	88	dep	months	673:678	arg1	FO					687:688	FO	687:688	FO	687:688	After that half of the animals continued with the SRD until month 8 while in the other half corn oil was replaced by FO for 2 months (SRD + FO).
29645025	3	88	dep	months	673:678	arg1	SRD					681:683	SRD	681:683	SRD	681:683	After that half of the animals continued with the SRD until month 8 while in the other half corn oil was replaced by FO for 2 months (SRD + FO).
29645025	7	89	theme	plasma	1395:1400	arg1	TNF-α					1402:1406	normal plasma TNF-α and IL-6 levels	1388:1422	TNF-α	1402:1406	Compared with the SRD the SRD + FO fed group showed a decrease of fat pad weight and the antioxidant and oxidant enzyme activities and ROS content returned to control values along with normal plasma TNF-α and IL-6 levels.
29645025	2	90	theme	Wistar	486:491	arg1	rats					493:496	Wistar rats	486:496	Wistar rats	486:496	Wistar rats were fed a sucrose rich diet (SRD) for 6 months.
29645025	10	91	theme	beneficial	1740:1749	arg1	effect					1751:1756	a beneficial effect	1738:1756	a beneficial effect	1738:1756	Finally, these findings reinforce the view that dietary FO may exert a beneficial effect in ameliorating the dyslipidemia and insulin resistance in this animal model.
29645025	1	92	theme	oxidative	400:408	arg1	stress					410:415	oxidative stress	400:415	oxidative stress	400:415	This work aims to assess the possible beneficial effects of dietary fish oil (FO) on the pre-existing adipose tissue dysfunction through the improvement or reversion of the mechanisms underlying oxidative stress and pro-inflammatory cytokines in dyslipemic insulin-resistant rats.
29645025	8	93	theme	expression	1506:1515	arg1	increase					1482:1489	the increase	1478:1489	the increase of protein and expression of UCP2	1478:1523	FO normalized both the decrease of PPARγ protein and the increase of protein and expression of UCP2.
29645025	8	93	theme	expression	1506:1515	arg1	decrease					1448:1455	the decrease	1444:1455	the decrease of PPARγ protein	1444:1472	FO normalized both the decrease of PPARγ protein and the increase of protein and expression of UCP2.
29645025	5	94	theme	TNF-α	1057:1061	arg1	levels					1079:1084	TNF-α and IL-6 plasma levels	1057:1084	levels	1079:1084	In an epididymal fat pad, we analyzed antioxidant and oxidant enzyme activities, ROS content, glutathione redox state, the protein level of peroxisome proliferator-activated receptor gamma (PPARγ) and the expression and protein levels of uncoupling protein 2 (UCP2) as well as oxidative stress biomarkers and TNF-α and IL-6 plasma levels.
29645025	5	95	theme	peroxisome	888:897	arg1	PPARγ					938:942	PPARγ	938:942	PPARγ	938:942	In an epididymal fat pad, we analyzed antioxidant and oxidant enzyme activities, ROS content, glutathione redox state, the protein level of peroxisome proliferator-activated receptor gamma (PPARγ) and the expression and protein levels of uncoupling protein 2 (UCP2) as well as oxidative stress biomarkers and TNF-α and IL-6 plasma levels.
29645025	5	95	theme	peroxisome	888:897	arg1	gamma					931:935	peroxisome proliferator-activated receptor gamma	888:935	peroxisome proliferator-activated receptor gamma (PPARγ)	888:943	In an epididymal fat pad, we analyzed antioxidant and oxidant enzyme activities, ROS content, glutathione redox state, the protein level of peroxisome proliferator-activated receptor gamma (PPARγ) and the expression and protein levels of uncoupling protein 2 (UCP2) as well as oxidative stress biomarkers and TNF-α and IL-6 plasma levels.
29645025	8	96	theme	PPARγ	1460:1464	arg1	protein					1466:1472	PPARγ protein	1460:1472	PPARγ protein	1460:1472	FO normalized both the decrease of PPARγ protein and the increase of protein and expression of UCP2.
29645025	8	97	theme	UCP2	1520:1523	arg1	expression					1506:1515	expression	1506:1515	expression	1506:1515	FO normalized both the decrease of PPARγ protein and the increase of protein and expression of UCP2.
29645025	8	97	theme	UCP2	1520:1523	arg1	protein					1494:1500	protein	1494:1500	protein	1494:1500	FO normalized both the decrease of PPARγ protein and the increase of protein and expression of UCP2.
29645025	1	98	theme	possible	234:241	arg1	effects					254:260	the possible beneficial effects	230:260	the possible beneficial effects of dietary fish oil (FO) on the pre-existing adipose tissue dysfunction	230:332	This work aims to assess the possible beneficial effects of dietary fish oil (FO) on the pre-existing adipose tissue dysfunction through the improvement or reversion of the mechanisms underlying oxidative stress and pro-inflammatory cytokines in dyslipemic insulin-resistant rats.
29645025	5	99	theme	receptor	922:929	arg1	PPARγ					938:942	PPARγ	938:942	PPARγ	938:942	In an epididymal fat pad, we analyzed antioxidant and oxidant enzyme activities, ROS content, glutathione redox state, the protein level of peroxisome proliferator-activated receptor gamma (PPARγ) and the expression and protein levels of uncoupling protein 2 (UCP2) as well as oxidative stress biomarkers and TNF-α and IL-6 plasma levels.
29645025	5	99	theme	receptor	922:929	arg1	gamma					931:935	peroxisome proliferator-activated receptor gamma	888:935	peroxisome proliferator-activated receptor gamma (PPARγ)	888:943	In an epididymal fat pad, we analyzed antioxidant and oxidant enzyme activities, ROS content, glutathione redox state, the protein level of peroxisome proliferator-activated receptor gamma (PPARγ) and the expression and protein levels of uncoupling protein 2 (UCP2) as well as oxidative stress biomarkers and TNF-α and IL-6 plasma levels.
30993386	8	0	theme	CS-altered	1175:1184	arg1	pathways					1206:1213	CS-altered microbial metabolic pathways	1175:1213	CS-altered microbial metabolic pathways that were linked to Glycerolipid metabolism, Biosynthesis of unsaturated fatty acids, sulfur reduction, and glutathione transport system	1175:1350	We identified CS-altered microbial metabolic pathways that were linked to Glycerolipid metabolism, Biosynthesis of unsaturated fatty acids, sulfur reduction, and glutathione transport system.
30993386	4	1	theme	HF	474:475	arg1	group					477:481	HF group	474:481	HF group	474:481	As compared to chow, mice in HF group demonstrated a significant increase in body weight (BW), fat mass (FM), together with total cholesterol (TC), and they even developed impaired glucose tolerance.
30993386	0	2	theme	weight	73:78	arg1	loss					80:83	weight loss	73:83	weight loss in mice	73:91	Structural modulation of gut microbiota reveals Coix seed contributes to weight loss in mice.
30993386	8	3	theme	unsaturated	1276:1286	arg1	acids					1294:1298	unsaturated fatty acids	1276:1298	unsaturated fatty acids	1276:1298	We identified CS-altered microbial metabolic pathways that were linked to Glycerolipid metabolism, Biosynthesis of unsaturated fatty acids, sulfur reduction, and glutathione transport system.
30993386	7	4	from	TC	1119:1120	arg1	data					1155:1158	our data	1151:1158	our data	1151:1158	A. muciniphila was reported to be inversely associated with obesity, diabetes and cardiometabolic diseases, while L. agilis was negatively associated with TC, BW, FM and blood glucose in our data.
30993386	8	5	theme	metabolic	1196:1204	arg1	pathways					1206:1213	CS-altered microbial metabolic pathways	1175:1213	CS-altered microbial metabolic pathways that were linked to Glycerolipid metabolism, Biosynthesis of unsaturated fatty acids, sulfur reduction, and glutathione transport system	1175:1350	We identified CS-altered microbial metabolic pathways that were linked to Glycerolipid metabolism, Biosynthesis of unsaturated fatty acids, sulfur reduction, and glutathione transport system.
30993386	4	6	dep	together	555:562	arg1	with					564:567	with	564:567	with	564:567	As compared to chow, mice in HF group demonstrated a significant increase in body weight (BW), fat mass (FM), together with total cholesterol (TC), and they even developed impaired glucose tolerance.
30993386	7	7	theme	L.	1078:1079	arg1	agilis					1081:1086	L. agilis	1078:1086	L. agilis	1078:1086	A. muciniphila was reported to be inversely associated with obesity, diabetes and cardiometabolic diseases, while L. agilis was negatively associated with TC, BW, FM and blood glucose in our data.
30993386	6	8	theme	sequencing	753:762	arg1	analysis					764:771	16S rRNA gene sequencing analysis	739:771	16S rRNA gene sequencing analysis	739:771	16S rRNA gene sequencing analysis revealed CS increased the abundance of genera Lactobacillus, Coprococcus, and Akkermansia in the gut microbita, and it also enriched species Akkermansia muciniphila and Lactobacillus agilis.
30993386	8	9	theme	microbial	1186:1194	arg1	pathways					1206:1213	CS-altered microbial metabolic pathways	1175:1213	CS-altered microbial metabolic pathways that were linked to Glycerolipid metabolism, Biosynthesis of unsaturated fatty acids, sulfur reduction, and glutathione transport system	1175:1350	We identified CS-altered microbial metabolic pathways that were linked to Glycerolipid metabolism, Biosynthesis of unsaturated fatty acids, sulfur reduction, and glutathione transport system.
30993386	1	10	used	used	119:122	arg2	seed					99:102	Coix seed	94:102	Coix seed (CS)	94:107	Coix seed (CS) is widely used as food material and herbal medicine in Asian countries with hypolipidemic and anti-inflammatory properties.
30993386	1	10	used	used	119:122	arg2	medicine					152:159	herbal medicine	145:159	herbal medicine	145:159	Coix seed (CS) is widely used as food material and herbal medicine in Asian countries with hypolipidemic and anti-inflammatory properties.
30993386	1	10	used	used	119:122	arg2	material					132:139	food material	127:139	food material	127:139	Coix seed (CS) is widely used as food material and herbal medicine in Asian countries with hypolipidemic and anti-inflammatory properties.
30993386	1	10	used	used	119:122	arg2	CS					105:106	CS	105:106	CS	105:106	Coix seed (CS) is widely used as food material and herbal medicine in Asian countries with hypolipidemic and anti-inflammatory properties.
30993386	6	11	theme	gene	748:751	arg1	analysis					764:771	16S rRNA gene sequencing analysis	739:771	16S rRNA gene sequencing analysis	739:771	16S rRNA gene sequencing analysis revealed CS increased the abundance of genera Lactobacillus, Coprococcus, and Akkermansia in the gut microbita, and it also enriched species Akkermansia muciniphila and Lactobacillus agilis.
30993386	6	12	theme	Lactobacillus	942:954	arg1	agilis					956:961	Lactobacillus agilis	942:961	Lactobacillus agilis	942:961	16S rRNA gene sequencing analysis revealed CS increased the abundance of genera Lactobacillus, Coprococcus, and Akkermansia in the gut microbita, and it also enriched species Akkermansia muciniphila and Lactobacillus agilis.
30993386	6	12	theme	Lactobacillus	942:954	arg1	species					906:912	species	906:912	species Akkermansia muciniphila and Lactobacillus agilis	906:961	16S rRNA gene sequencing analysis revealed CS increased the abundance of genera Lactobacillus, Coprococcus, and Akkermansia in the gut microbita, and it also enriched species Akkermansia muciniphila and Lactobacillus agilis.
30993386	6	13	dep	species	906:912	arg1	muciniphila					926:936	Akkermansia muciniphila	914:936	Akkermansia muciniphila	914:936	16S rRNA gene sequencing analysis revealed CS increased the abundance of genera Lactobacillus, Coprococcus, and Akkermansia in the gut microbita, and it also enriched species Akkermansia muciniphila and Lactobacillus agilis.
30993386	6	13	dep	species	906:912	arg1	agilis					956:961	Lactobacillus agilis	942:961	Lactobacillus agilis	942:961	16S rRNA gene sequencing analysis revealed CS increased the abundance of genera Lactobacillus, Coprococcus, and Akkermansia in the gut microbita, and it also enriched species Akkermansia muciniphila and Lactobacillus agilis.
30993386	6	13	dep	species	906:912	arg1	species					906:912	species	906:912	species Akkermansia muciniphila and Lactobacillus agilis	906:961	16S rRNA gene sequencing analysis revealed CS increased the abundance of genera Lactobacillus, Coprococcus, and Akkermansia in the gut microbita, and it also enriched species Akkermansia muciniphila and Lactobacillus agilis.
30993386	1	14	theme	food	127:130	arg1	seed					99:102	Coix seed	94:102	Coix seed (CS)	94:107	Coix seed (CS) is widely used as food material and herbal medicine in Asian countries with hypolipidemic and anti-inflammatory properties.
30993386	1	14	theme	food	127:130	arg1	material					132:139	food material	127:139	food material	127:139	Coix seed (CS) is widely used as food material and herbal medicine in Asian countries with hypolipidemic and anti-inflammatory properties.
30993386	6	15	theme	rRNA	743:746	arg1	analysis					764:771	16S rRNA gene sequencing analysis	739:771	16S rRNA gene sequencing analysis	739:771	16S rRNA gene sequencing analysis revealed CS increased the abundance of genera Lactobacillus, Coprococcus, and Akkermansia in the gut microbita, and it also enriched species Akkermansia muciniphila and Lactobacillus agilis.
30993386	6	16	dep	revealed	773:780	arg1	increased					785:793	increased	785:793	revealed CS increased the abundance of genera Lactobacillus, Coprococcus, and Akkermansia in the gut microbita	773:882	16S rRNA gene sequencing analysis revealed CS increased the abundance of genera Lactobacillus, Coprococcus, and Akkermansia in the gut microbita, and it also enriched species Akkermansia muciniphila and Lactobacillus agilis.
30993386	0	17	theme	Structural	0:9	arg1	modulation					11:20	Structural modulation	0:20	Structural modulation of gut microbiota	0:38	Structural modulation of gut microbiota reveals Coix seed contributes to weight loss in mice.
30993386	3	18	theme	standard	395:402	arg1	chow					404:407	standard chow	395:407	standard chow	395:407	Here, three groups of mice were fed different diets for 5 weeks: standard chow, high fat (HF), and CS added to HF.
30993386	4	19	from	mice	466:469	arg1	group					477:481	HF group	474:481	HF group	474:481	As compared to chow, mice in HF group demonstrated a significant increase in body weight (BW), fat mass (FM), together with total cholesterol (TC), and they even developed impaired glucose tolerance.
30993386	6	20	theme	16S	739:741	arg1	rRNA					743:746	16S rRNA	739:746	16S rRNA gene sequencing analysis	739:771	16S rRNA gene sequencing analysis revealed CS increased the abundance of genera Lactobacillus, Coprococcus, and Akkermansia in the gut microbita, and it also enriched species Akkermansia muciniphila and Lactobacillus agilis.
30993386	7	21	from	FM	1127:1128	arg1	data					1155:1158	our data	1151:1158	our data	1151:1158	A. muciniphila was reported to be inversely associated with obesity, diabetes and cardiometabolic diseases, while L. agilis was negatively associated with TC, BW, FM and blood glucose in our data.
30993386	3	22	theme	mice	352:355	arg1	mice					352:355	mice	352:355	mice	352:355	Here, three groups of mice were fed different diets for 5 weeks: standard chow, high fat (HF), and CS added to HF.
30993386	3	22	theme	mice	352:355	arg1	groups					342:347	three groups	336:347	three groups of mice	336:355	Here, three groups of mice were fed different diets for 5 weeks: standard chow, high fat (HF), and CS added to HF.
30993386	1	23	theme	herbal	145:150	arg1	seed					99:102	Coix seed	94:102	Coix seed (CS)	94:107	Coix seed (CS) is widely used as food material and herbal medicine in Asian countries with hypolipidemic and anti-inflammatory properties.
30993386	1	23	theme	herbal	145:150	arg1	medicine					152:159	herbal medicine	145:159	herbal medicine	145:159	Coix seed (CS) is widely used as food material and herbal medicine in Asian countries with hypolipidemic and anti-inflammatory properties.
30993386	0	24	theme	gut	25:27	arg1	microbiota					29:38	gut microbiota	25:38	gut microbiota	25:38	Structural modulation of gut microbiota reveals Coix seed contributes to weight loss in mice.
30993386	5	25	theme	HF-mediated	651:661	arg1	effects					685:691	These HF-mediated deleterious metabolic effects	645:691	These HF-mediated deleterious metabolic effects	645:691	These HF-mediated deleterious metabolic effects were counteracted partly by complementing CS.
30993386	7	26	from	BW	1123:1124	arg1	data					1155:1158	our data	1151:1158	our data	1151:1158	A. muciniphila was reported to be inversely associated with obesity, diabetes and cardiometabolic diseases, while L. agilis was negatively associated with TC, BW, FM and blood glucose in our data.
30993386	8	27	theme	Glycerolipid	1235:1246	arg1	metabolism					1248:1257	Glycerolipid metabolism	1235:1257	Glycerolipid metabolism	1235:1257	We identified CS-altered microbial metabolic pathways that were linked to Glycerolipid metabolism, Biosynthesis of unsaturated fatty acids, sulfur reduction, and glutathione transport system.
30993386	3	28	theme	high	410:413	arg1	HF					420:421	HF	420:421	HF	420:421	Here, three groups of mice were fed different diets for 5 weeks: standard chow, high fat (HF), and CS added to HF.
30993386	3	28	theme	high	410:413	arg1	fat					415:417	high fat	410:417	high fat (HF)	410:422	Here, three groups of mice were fed different diets for 5 weeks: standard chow, high fat (HF), and CS added to HF.
30993386	5	29	theme	metabolic	675:683	arg1	effects					685:691	These HF-mediated deleterious metabolic effects	645:691	These HF-mediated deleterious metabolic effects	645:691	These HF-mediated deleterious metabolic effects were counteracted partly by complementing CS.
30993386	8	30	attach	linked	1225:1230	arg1	Biosynthesis					1260:1271	Biosynthesis	1260:1271	Biosynthesis of unsaturated fatty acids	1260:1298	We identified CS-altered microbial metabolic pathways that were linked to Glycerolipid metabolism, Biosynthesis of unsaturated fatty acids, sulfur reduction, and glutathione transport system.
30993386	8	30	attach	linked	1225:1230	arg1	metabolism					1248:1257	Glycerolipid metabolism	1235:1257	Glycerolipid metabolism	1235:1257	We identified CS-altered microbial metabolic pathways that were linked to Glycerolipid metabolism, Biosynthesis of unsaturated fatty acids, sulfur reduction, and glutathione transport system.
30993386	8	30	attach	linked	1225:1230	arg1	system					1345:1350	glutathione transport system	1323:1350	glutathione transport system	1323:1350	We identified CS-altered microbial metabolic pathways that were linked to Glycerolipid metabolism, Biosynthesis of unsaturated fatty acids, sulfur reduction, and glutathione transport system.
30993386	8	30	attach	linked	1225:1230	arg1	reduction					1308:1316	sulfur reduction	1301:1316	sulfur reduction	1301:1316	We identified CS-altered microbial metabolic pathways that were linked to Glycerolipid metabolism, Biosynthesis of unsaturated fatty acids, sulfur reduction, and glutathione transport system.
30993386	8	30	attach	linked	1225:1230	arg2	pathways					1206:1213	CS-altered microbial metabolic pathways	1175:1213	CS-altered microbial metabolic pathways that were linked to Glycerolipid metabolism, Biosynthesis of unsaturated fatty acids, sulfur reduction, and glutathione transport system	1175:1350	We identified CS-altered microbial metabolic pathways that were linked to Glycerolipid metabolism, Biosynthesis of unsaturated fatty acids, sulfur reduction, and glutathione transport system.
30993386	1	31	with	countries	170:178	arg1	properties					221:230	hypolipidemic and anti-inflammatory properties	185:230	properties	221:230	Coix seed (CS) is widely used as food material and herbal medicine in Asian countries with hypolipidemic and anti-inflammatory properties.
30993386	0	32	from	loss	80:83	arg1	mice					88:91	mice	88:91	mice	88:91	Structural modulation of gut microbiota reveals Coix seed contributes to weight loss in mice.
30993386	1	33	theme	Coix	94:97	arg1	CS					105:106	CS	105:106	CS	105:106	Coix seed (CS) is widely used as food material and herbal medicine in Asian countries with hypolipidemic and anti-inflammatory properties.
30993386	1	33	theme	Coix	94:97	arg1	seed					99:102	Coix seed	94:102	Coix seed (CS)	94:107	Coix seed (CS) is widely used as food material and herbal medicine in Asian countries with hypolipidemic and anti-inflammatory properties.
30993386	1	33	theme	Coix	94:97	arg1	material					132:139	food material	127:139	food material	127:139	Coix seed (CS) is widely used as food material and herbal medicine in Asian countries with hypolipidemic and anti-inflammatory properties.
30993386	1	33	theme	Coix	94:97	arg1	medicine					152:159	herbal medicine	145:159	herbal medicine	145:159	Coix seed (CS) is widely used as food material and herbal medicine in Asian countries with hypolipidemic and anti-inflammatory properties.
30993386	4	34	theme	body	522:525	arg1	BW					535:536	BW	535:536	BW	535:536	As compared to chow, mice in HF group demonstrated a significant increase in body weight (BW), fat mass (FM), together with total cholesterol (TC), and they even developed impaired glucose tolerance.
30993386	4	34	theme	body	522:525	arg1	weight					527:532	body weight	522:532	body weight (BW)	522:537	As compared to chow, mice in HF group demonstrated a significant increase in body weight (BW), fat mass (FM), together with total cholesterol (TC), and they even developed impaired glucose tolerance.
30993386	3	35	theme	different	366:374	arg1	diets					376:380	different diets	366:380	different diets	366:380	Here, three groups of mice were fed different diets for 5 weeks: standard chow, high fat (HF), and CS added to HF.
30993386	7	36	theme	blood	1134:1138	arg1	glucose					1140:1146	blood glucose	1134:1146	blood glucose	1134:1146	A. muciniphila was reported to be inversely associated with obesity, diabetes and cardiometabolic diseases, while L. agilis was negatively associated with TC, BW, FM and blood glucose in our data.
30993386	7	37	theme	cardiometabolic	1046:1060	arg1	diseases					1062:1069	cardiometabolic diseases	1046:1069	cardiometabolic diseases	1046:1069	A. muciniphila was reported to be inversely associated with obesity, diabetes and cardiometabolic diseases, while L. agilis was negatively associated with TC, BW, FM and blood glucose in our data.
30993386	9	38	theme	metabolic	1452:1460	arg1	disorders					1462:1470	obesity-related metabolic disorders	1436:1470	obesity-related metabolic disorders	1436:1470	Our results indicate CS may be used as prebiotic agents to lose weight and prevent obesity-related metabolic disorders.
30993386	4	39	theme	fat	540:542	arg1	FM					550:551	FM	550:551	FM	550:551	As compared to chow, mice in HF group demonstrated a significant increase in body weight (BW), fat mass (FM), together with total cholesterol (TC), and they even developed impaired glucose tolerance.
30993386	4	39	theme	fat	540:542	arg1	mass					544:547	fat mass	540:547	fat mass (FM)	540:552	As compared to chow, mice in HF group demonstrated a significant increase in body weight (BW), fat mass (FM), together with total cholesterol (TC), and they even developed impaired glucose tolerance.
30993386	1	40	theme	Asian	164:168	arg1	countries					170:178	Asian countries	164:178	Asian countries with hypolipidemic and anti-inflammatory properties	164:230	Coix seed (CS) is widely used as food material and herbal medicine in Asian countries with hypolipidemic and anti-inflammatory properties.
30993386	0	41	theme	microbiota	29:38	arg1	modulation					11:20	Structural modulation	0:20	Structural modulation of gut microbiota	0:38	Structural modulation of gut microbiota reveals Coix seed contributes to weight loss in mice.
30993386	6	42	theme	genera	812:817	arg1	abundance					799:807	the abundance	795:807	the abundance of genera Lactobacillus, Coprococcus, and Akkermansia in the gut microbita	795:882	16S rRNA gene sequencing analysis revealed CS increased the abundance of genera Lactobacillus, Coprococcus, and Akkermansia in the gut microbita, and it also enriched species Akkermansia muciniphila and Lactobacillus agilis.
30993386	6	43	dep	genera	812:817	arg1	Coprococcus					834:844	Coprococcus	834:844	Coprococcus	834:844	16S rRNA gene sequencing analysis revealed CS increased the abundance of genera Lactobacillus, Coprococcus, and Akkermansia in the gut microbita, and it also enriched species Akkermansia muciniphila and Lactobacillus agilis.
30993386	6	43	dep	genera	812:817	arg1	Akkermansia					851:861	Akkermansia	851:861	Akkermansia	851:861	16S rRNA gene sequencing analysis revealed CS increased the abundance of genera Lactobacillus, Coprococcus, and Akkermansia in the gut microbita, and it also enriched species Akkermansia muciniphila and Lactobacillus agilis.
30993386	6	43	dep	genera	812:817	arg1	Lactobacillus					819:831	Lactobacillus	819:831	Lactobacillus	819:831	16S rRNA gene sequencing analysis revealed CS increased the abundance of genera Lactobacillus, Coprococcus, and Akkermansia in the gut microbita, and it also enriched species Akkermansia muciniphila and Lactobacillus agilis.
30993386	5	44	theme	deleterious	663:673	arg1	effects					685:691	These HF-mediated deleterious metabolic effects	645:691	These HF-mediated deleterious metabolic effects	645:691	These HF-mediated deleterious metabolic effects were counteracted partly by complementing CS.
30993386	7	45	from	glucose	1140:1146	arg1	data					1155:1158	our data	1151:1158	our data	1151:1158	A. muciniphila was reported to be inversely associated with obesity, diabetes and cardiometabolic diseases, while L. agilis was negatively associated with TC, BW, FM and blood glucose in our data.
30993386	6	46	from	abundance	799:807	arg1	microbita					874:882	the gut microbita	866:882	the gut microbita	866:882	16S rRNA gene sequencing analysis revealed CS increased the abundance of genera Lactobacillus, Coprococcus, and Akkermansia in the gut microbita, and it also enriched species Akkermansia muciniphila and Lactobacillus agilis.
30993386	8	47	theme	fatty	1288:1292	arg1	acids					1294:1298	unsaturated fatty acids	1276:1298	unsaturated fatty acids	1276:1298	We identified CS-altered microbial metabolic pathways that were linked to Glycerolipid metabolism, Biosynthesis of unsaturated fatty acids, sulfur reduction, and glutathione transport system.
30993386	6	48	theme	Akkermansia	914:924	arg1	muciniphila					926:936	Akkermansia muciniphila	914:936	Akkermansia muciniphila	914:936	16S rRNA gene sequencing analysis revealed CS increased the abundance of genera Lactobacillus, Coprococcus, and Akkermansia in the gut microbita, and it also enriched species Akkermansia muciniphila and Lactobacillus agilis.
30993386	6	48	theme	Akkermansia	914:924	arg1	species					906:912	species	906:912	species Akkermansia muciniphila and Lactobacillus agilis	906:961	16S rRNA gene sequencing analysis revealed CS increased the abundance of genera Lactobacillus, Coprococcus, and Akkermansia in the gut microbita, and it also enriched species Akkermansia muciniphila and Lactobacillus agilis.
30993386	8	49	theme	transport	1335:1343	arg1	system					1345:1350	glutathione transport system	1323:1350	glutathione transport system	1323:1350	We identified CS-altered microbial metabolic pathways that were linked to Glycerolipid metabolism, Biosynthesis of unsaturated fatty acids, sulfur reduction, and glutathione transport system.
30993386	2	50	theme	microbiota	302:311	arg1	composition					279:289	the composition	275:289	the composition of the gut microbiota	275:311	But whether CS takes effect by modulating the composition of the gut microbiota remains unknown.
30993386	8	51	theme	sulfur	1301:1306	arg1	reduction					1308:1316	sulfur reduction	1301:1316	sulfur reduction	1301:1316	We identified CS-altered microbial metabolic pathways that were linked to Glycerolipid metabolism, Biosynthesis of unsaturated fatty acids, sulfur reduction, and glutathione transport system.
30993386	8	52	theme	acids	1294:1298	arg1	Biosynthesis					1260:1271	Biosynthesis	1260:1271	Biosynthesis of unsaturated fatty acids	1260:1298	We identified CS-altered microbial metabolic pathways that were linked to Glycerolipid metabolism, Biosynthesis of unsaturated fatty acids, sulfur reduction, and glutathione transport system.
30993386	8	52	theme	acids	1294:1298	arg1	metabolism					1248:1257	Glycerolipid metabolism	1235:1257	Glycerolipid metabolism	1235:1257	We identified CS-altered microbial metabolic pathways that were linked to Glycerolipid metabolism, Biosynthesis of unsaturated fatty acids, sulfur reduction, and glutathione transport system.
30993386	8	52	theme	acids	1294:1298	arg1	system					1345:1350	glutathione transport system	1323:1350	glutathione transport system	1323:1350	We identified CS-altered microbial metabolic pathways that were linked to Glycerolipid metabolism, Biosynthesis of unsaturated fatty acids, sulfur reduction, and glutathione transport system.
30993386	8	52	theme	acids	1294:1298	arg1	reduction					1308:1316	sulfur reduction	1301:1316	sulfur reduction	1301:1316	We identified CS-altered microbial metabolic pathways that were linked to Glycerolipid metabolism, Biosynthesis of unsaturated fatty acids, sulfur reduction, and glutathione transport system.
30993386	8	53	theme	glutathione	1323:1333	arg1	system					1345:1350	glutathione transport system	1323:1350	glutathione transport system	1323:1350	We identified CS-altered microbial metabolic pathways that were linked to Glycerolipid metabolism, Biosynthesis of unsaturated fatty acids, sulfur reduction, and glutathione transport system.
30993386	4	54	theme	glucose	626:632	arg1	tolerance					634:642	impaired glucose tolerance	617:642	impaired glucose tolerance	617:642	As compared to chow, mice in HF group demonstrated a significant increase in body weight (BW), fat mass (FM), together with total cholesterol (TC), and they even developed impaired glucose tolerance.
30993386	2	55	theme	gut	298:300	arg1	microbiota					302:311	the gut microbiota	294:311	the gut microbiota	294:311	But whether CS takes effect by modulating the composition of the gut microbiota remains unknown.
30993386	9	56	used	used	1384:1387	arg2	CS					1374:1375	CS	1374:1375	CS	1374:1375	Our results indicate CS may be used as prebiotic agents to lose weight and prevent obesity-related metabolic disorders.
30993386	9	56	used	used	1384:1387	arg2	agents					1402:1407	prebiotic agents	1392:1407	prebiotic agents	1392:1407	Our results indicate CS may be used as prebiotic agents to lose weight and prevent obesity-related metabolic disorders.
30993386	4	57	theme	significant	498:508	arg1	increase					510:517	a significant increase	496:517	a significant increase in body weight (BW), fat mass (FM), together with total cholesterol (TC)	496:590	As compared to chow, mice in HF group demonstrated a significant increase in body weight (BW), fat mass (FM), together with total cholesterol (TC), and they even developed impaired glucose tolerance.
30993386	9	58	theme	prebiotic	1392:1400	arg1	CS					1374:1375	CS	1374:1375	CS	1374:1375	Our results indicate CS may be used as prebiotic agents to lose weight and prevent obesity-related metabolic disorders.
30993386	9	58	theme	prebiotic	1392:1400	arg1	agents					1402:1407	prebiotic agents	1392:1407	prebiotic agents	1392:1407	Our results indicate CS may be used as prebiotic agents to lose weight and prevent obesity-related metabolic disorders.
30993386	4	59	theme	impaired	617:624	arg1	tolerance					634:642	impaired glucose tolerance	617:642	impaired glucose tolerance	617:642	As compared to chow, mice in HF group demonstrated a significant increase in body weight (BW), fat mass (FM), together with total cholesterol (TC), and they even developed impaired glucose tolerance.
30993386	4	60	theme	total	569:573	arg1	TC					588:589	TC	588:589	TC	588:589	As compared to chow, mice in HF group demonstrated a significant increase in body weight (BW), fat mass (FM), together with total cholesterol (TC), and they even developed impaired glucose tolerance.
30993386	4	60	theme	total	569:573	arg1	cholesterol					575:585	total cholesterol	569:585	total cholesterol (TC)	569:590	As compared to chow, mice in HF group demonstrated a significant increase in body weight (BW), fat mass (FM), together with total cholesterol (TC), and they even developed impaired glucose tolerance.
30993386	1	61	theme	hypolipidemic	185:197	arg1	properties					221:230	hypolipidemic and anti-inflammatory properties	185:230	properties	221:230	Coix seed (CS) is widely used as food material and herbal medicine in Asian countries with hypolipidemic and anti-inflammatory properties.
30993386	9	62	theme	obesity-related	1436:1450	arg1	disorders					1462:1470	obesity-related metabolic disorders	1436:1470	obesity-related metabolic disorders	1436:1470	Our results indicate CS may be used as prebiotic agents to lose weight and prevent obesity-related metabolic disorders.
30993386	4	63	from	increase	510:517	arg1	weight					527:532	body weight	522:532	body weight (BW)	522:537	As compared to chow, mice in HF group demonstrated a significant increase in body weight (BW), fat mass (FM), together with total cholesterol (TC), and they even developed impaired glucose tolerance.
30993386	4	63	from	increase	510:517	arg1	cholesterol					575:585	total cholesterol	569:585	total cholesterol (TC)	569:590	As compared to chow, mice in HF group demonstrated a significant increase in body weight (BW), fat mass (FM), together with total cholesterol (TC), and they even developed impaired glucose tolerance.
30993386	4	63	from	increase	510:517	arg1	BW					535:536	BW	535:536	BW	535:536	As compared to chow, mice in HF group demonstrated a significant increase in body weight (BW), fat mass (FM), together with total cholesterol (TC), and they even developed impaired glucose tolerance.
30993386	4	63	from	increase	510:517	arg1	FM					550:551	FM	550:551	FM	550:551	As compared to chow, mice in HF group demonstrated a significant increase in body weight (BW), fat mass (FM), together with total cholesterol (TC), and they even developed impaired glucose tolerance.
30993386	4	63	from	increase	510:517	arg1	TC					588:589	TC	588:589	TC	588:589	As compared to chow, mice in HF group demonstrated a significant increase in body weight (BW), fat mass (FM), together with total cholesterol (TC), and they even developed impaired glucose tolerance.
30993386	4	63	from	increase	510:517	arg1	mass					544:547	fat mass	540:547	fat mass (FM)	540:552	As compared to chow, mice in HF group demonstrated a significant increase in body weight (BW), fat mass (FM), together with total cholesterol (TC), and they even developed impaired glucose tolerance.
30993386	6	64	theme	gut	870:872	arg1	microbita					874:882	the gut microbita	866:882	the gut microbita	866:882	16S rRNA gene sequencing analysis revealed CS increased the abundance of genera Lactobacillus, Coprococcus, and Akkermansia in the gut microbita, and it also enriched species Akkermansia muciniphila and Lactobacillus agilis.
30993386	1	65	theme	anti-inflammatory	203:219	arg1	properties					221:230	hypolipidemic and anti-inflammatory properties	185:230	properties	221:230	Coix seed (CS) is widely used as food material and herbal medicine in Asian countries with hypolipidemic and anti-inflammatory properties.
30806616	3	0	theme	rRNA	313:316	arg1	sequences					323:331	16S rRNA gene sequences	309:331	16S rRNA gene sequences	309:331	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain UJ94T shared sequence similarities of 98.4, 96.1 and 95.4 % with Tamlana agarivorans JW-26T, Tamlana sedimentorum KMM 9545T and Tamlana crocina HST1-43T, respectively.
30806616	2	1	theme	non-motile	125:134	arg1	strain					190:195	A Gram-stain-negative, aerobic, non-motile, rod-shaped, agarolytic and carrageenolytic bacterial strain	93:195	A Gram-stain-negative, aerobic, non-motile, rod-shaped, agarolytic and carrageenolytic bacterial strain	93:195	A Gram-stain-negative, aerobic, non-motile, rod-shaped, agarolytic and carrageenolytic bacterial strain, designated UJ94T, was isolated from seawater of Uljin in the Republic of Korea.
30806616	8	2	with	%	1132:1132	arg1	strains					1163:1169	the type strains	1154:1169	the type strains of T. agarivorans and T. sedimentorum	1154:1207	The ranges of average nucleotide identity and in silico DNA-DNA hybridization estimated by genome-to-genome distance were 90.6-74.2 % and 47.6-14.6 %, respectively, with the type strains of T. agarivorans and T. sedimentorum.
30806616	3	3	theme	crocina	490:496	arg1	HST1-43T					498:505	Tamlana crocina HST1-43T	482:505	Tamlana crocina HST1-43T	482:505	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain UJ94T shared sequence similarities of 98.4, 96.1 and 95.4 % with Tamlana agarivorans JW-26T, Tamlana sedimentorum KMM 9545T and Tamlana crocina HST1-43T, respectively.
30806616	5	4	theme	major	625:629	arg1	acids					637:641	The major fatty acids	621:641	The major fatty acids of strain UJ94T	621:657	The major fatty acids of strain UJ94T were iso-C15 : 0, summed feature 3 (C16 : 1ω7c/C16 : 1ω6c) and iso-C17 : 0 3-OH; MK-6 was the predominant menaquinone.
30806616	5	4	theme	major	625:629	arg1	iso-C15 					664:671	iso-C15 	664:671	iso-C15 	664:671	The major fatty acids of strain UJ94T were iso-C15 : 0, summed feature 3 (C16 : 1ω7c/C16 : 1ω6c) and iso-C17 : 0 3-OH; MK-6 was the predominant menaquinone.
30806616	9	5	theme	strain	1325:1330	arg1	UJ94T					1332:1336	strain UJ94T	1325:1336	strain UJ94T	1325:1336	The present polyphasic study, including phylogenetic, chemotaxonomic, biochemical and genomic data, suggested that strain UJ94T represents a novel species of the genus Tamlana, for which the name Tamlana carrageenivorans sp.
30806616	3	6	theme	gene	318:321	arg1	sequences					323:331	16S rRNA gene sequences	309:331	16S rRNA gene sequences	309:331	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain UJ94T shared sequence similarities of 98.4, 96.1 and 95.4 % with Tamlana agarivorans JW-26T, Tamlana sedimentorum KMM 9545T and Tamlana crocina HST1-43T, respectively.
30806616	9	7	theme	present	1214:1220	arg1	study					1233:1237	The present polyphasic study	1210:1237	The present polyphasic study	1210:1237	The present polyphasic study, including phylogenetic, chemotaxonomic, biochemical and genomic data, suggested that strain UJ94T represents a novel species of the genus Tamlana, for which the name Tamlana carrageenivorans sp.
30806616	3	8	with	HST1-43T	498:505	arg1	Tamlana					447:453	Tamlana	447:453	Tamlana	447:453	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain UJ94T shared sequence similarities of 98.4, 96.1 and 95.4 % with Tamlana agarivorans JW-26T, Tamlana sedimentorum KMM 9545T and Tamlana crocina HST1-43T, respectively.
30806616	3	8	with	HST1-43T	498:505	arg1	9545T					472:476	9545T	472:476	9545T	472:476	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain UJ94T shared sequence similarities of 98.4, 96.1 and 95.4 % with Tamlana agarivorans JW-26T, Tamlana sedimentorum KMM 9545T and Tamlana crocina HST1-43T, respectively.
30806616	3	9	theme	HST1-43T	498:505	arg1	similarities					376:387	sequence similarities	367:387	sequence similarities of 98.4, 96.1 and 95.4 % with Tamlana agarivorans JW-26T, Tamlana sedimentorum KMM 9545T and Tamlana crocina HST1-43T, respectively	367:519	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain UJ94T shared sequence similarities of 98.4, 96.1 and 95.4 % with Tamlana agarivorans JW-26T, Tamlana sedimentorum KMM 9545T and Tamlana crocina HST1-43T, respectively.
30806616	8	10	theme	genome-to-genome	1075:1090	arg1	distance					1092:1099	genome-to-genome distance	1075:1099	genome-to-genome distance	1075:1099	The ranges of average nucleotide identity and in silico DNA-DNA hybridization estimated by genome-to-genome distance were 90.6-74.2 % and 47.6-14.6 %, respectively, with the type strains of T. agarivorans and T. sedimentorum.
30806616	2	11	theme	aerobic	116:122	arg1	strain					190:195	A Gram-stain-negative, aerobic, non-motile, rod-shaped, agarolytic and carrageenolytic bacterial strain	93:195	A Gram-stain-negative, aerobic, non-motile, rod-shaped, agarolytic and carrageenolytic bacterial strain	93:195	A Gram-stain-negative, aerobic, non-motile, rod-shaped, agarolytic and carrageenolytic bacterial strain, designated UJ94T, was isolated from seawater of Uljin in the Republic of Korea.
30806616	2	12	attach	isolated	220:227	arg1	seawater					234:241	seawater	234:241	seawater of Uljin in the Republic of Korea	234:275	A Gram-stain-negative, aerobic, non-motile, rod-shaped, agarolytic and carrageenolytic bacterial strain, designated UJ94T, was isolated from seawater of Uljin in the Republic of Korea.
30806616	2	12	attach	isolated	220:227	arg2	strain					190:195	A Gram-stain-negative, aerobic, non-motile, rod-shaped, agarolytic and carrageenolytic bacterial strain	93:195	A Gram-stain-negative, aerobic, non-motile, rod-shaped, agarolytic and carrageenolytic bacterial strain	93:195	A Gram-stain-negative, aerobic, non-motile, rod-shaped, agarolytic and carrageenolytic bacterial strain, designated UJ94T, was isolated from seawater of Uljin in the Republic of Korea.
30806616	6	13	theme	polar	885:889	arg1	Phosphatidylethanolamine					778:801	Phosphatidylethanolamine	778:801	Phosphatidylethanolamine	778:801	Phosphatidylethanolamine, two unidentified aminolipids and five unidentified lipids were detected as major polar lipids.
30806616	6	13	theme	polar	885:889	arg1	lipids					891:896	major polar lipids	879:896	major polar lipids	879:896	Phosphatidylethanolamine, two unidentified aminolipids and five unidentified lipids were detected as major polar lipids.
30806616	5	14	theme	UJ94T	653:657	arg1	acids					637:641	The major fatty acids	621:641	The major fatty acids of strain UJ94T	621:657	The major fatty acids of strain UJ94T were iso-C15 : 0, summed feature 3 (C16 : 1ω7c/C16 : 1ω6c) and iso-C17 : 0 3-OH; MK-6 was the predominant menaquinone.
30806616	5	14	theme	UJ94T	653:657	arg1	iso-C15 					664:671	iso-C15 	664:671	iso-C15 	664:671	The major fatty acids of strain UJ94T were iso-C15 : 0, summed feature 3 (C16 : 1ω7c/C16 : 1ω6c) and iso-C17 : 0 3-OH; MK-6 was the predominant menaquinone.
30806616	8	15	theme	identity	1017:1024	arg1	ranges					988:993	The ranges	984:993	The ranges of average nucleotide identity and in silico DNA-DNA hybridization estimated by genome-to-genome distance	984:1099	The ranges of average nucleotide identity and in silico DNA-DNA hybridization estimated by genome-to-genome distance were 90.6-74.2 % and 47.6-14.6 %, respectively, with the type strains of T. agarivorans and T. sedimentorum.
30806616	8	15	theme	identity	1017:1024	arg1	%					1116:1116	90.6-74.2 %	1106:1116	90.6-74.2 %	1106:1116	The ranges of average nucleotide identity and in silico DNA-DNA hybridization estimated by genome-to-genome distance were 90.6-74.2 % and 47.6-14.6 %, respectively, with the type strains of T. agarivorans and T. sedimentorum.
30806616	9	16	theme	genus	1372:1376	arg1	Tamlana					1378:1384	the genus Tamlana	1368:1384	the genus Tamlana	1368:1384	The present polyphasic study, including phylogenetic, chemotaxonomic, biochemical and genomic data, suggested that strain UJ94T represents a novel species of the genus Tamlana, for which the name Tamlana carrageenivorans sp.
30806616	4	17	located	observed	549:556	arg1	pH					573:574	pH 6.5-8.0	573:582	pH 6.5-8.0	573:582	Growth of strain UJ94T was observed at 4-37 °C and pH 6.5-8.0 in the presence of 2-9 % (w/v) NaCl.
30806616	4	17	located	observed	549:556	arg1	4-37 °C					561:567	4-37 °C	561:567	4-37 °C	561:567	Growth of strain UJ94T was observed at 4-37 °C and pH 6.5-8.0 in the presence of 2-9 % (w/v) NaCl.
30806616	4	17	located	observed	549:556	arg1	presence					591:598	the presence	587:598	the presence of 2-9 % (w/v) NaCl	587:618	Growth of strain UJ94T was observed at 4-37 °C and pH 6.5-8.0 in the presence of 2-9 % (w/v) NaCl.
30806616	4	17	located	observed	549:556	arg2	Growth					522:527	Growth	522:527	Growth of strain UJ94T	522:543	Growth of strain UJ94T was observed at 4-37 °C and pH 6.5-8.0 in the presence of 2-9 % (w/v) NaCl.
30806616	2	18	theme	agarolytic	149:158	arg1	strain					190:195	A Gram-stain-negative, aerobic, non-motile, rod-shaped, agarolytic and carrageenolytic bacterial strain	93:195	A Gram-stain-negative, aerobic, non-motile, rod-shaped, agarolytic and carrageenolytic bacterial strain	93:195	A Gram-stain-negative, aerobic, non-motile, rod-shaped, agarolytic and carrageenolytic bacterial strain, designated UJ94T, was isolated from seawater of Uljin in the Republic of Korea.
30806616	5	19	theme	3-OH	734:737	arg1	MK-6					740:743	iso-C17 : 0 3-OH; MK-6	722:743	iso-C17 : 0 3-OH; MK-6	722:743	The major fatty acids of strain UJ94T were iso-C15 : 0, summed feature 3 (C16 : 1ω7c/C16 : 1ω6c) and iso-C17 : 0 3-OH; MK-6 was the predominant menaquinone.
30806616	5	19	theme	3-OH	734:737	arg1	 0					673:674	 0	673:674	 0	673:674	The major fatty acids of strain UJ94T were iso-C15 : 0, summed feature 3 (C16 : 1ω7c/C16 : 1ω6c) and iso-C17 : 0 3-OH; MK-6 was the predominant menaquinone.
30806616	5	19	theme	3-OH	734:737	arg1	menaquinone					765:775	the predominant menaquinone	749:775	the predominant menaquinone	749:775	The major fatty acids of strain UJ94T were iso-C15 : 0, summed feature 3 (C16 : 1ω7c/C16 : 1ω6c) and iso-C17 : 0 3-OH; MK-6 was the predominant menaquinone.
30806616	2	20	theme	Korea	271:275	arg1	Republic					259:266	the Republic	255:266	the Republic of Korea	255:275	A Gram-stain-negative, aerobic, non-motile, rod-shaped, agarolytic and carrageenolytic bacterial strain, designated UJ94T, was isolated from seawater of Uljin in the Republic of Korea.
30806616	5	21	theme	predominant	753:763	arg1	MK-6					740:743	iso-C17 : 0 3-OH; MK-6	722:743	iso-C17 : 0 3-OH; MK-6	722:743	The major fatty acids of strain UJ94T were iso-C15 : 0, summed feature 3 (C16 : 1ω7c/C16 : 1ω6c) and iso-C17 : 0 3-OH; MK-6 was the predominant menaquinone.
30806616	5	21	theme	predominant	753:763	arg1	 0					673:674	 0	673:674	 0	673:674	The major fatty acids of strain UJ94T were iso-C15 : 0, summed feature 3 (C16 : 1ω7c/C16 : 1ω6c) and iso-C17 : 0 3-OH; MK-6 was the predominant menaquinone.
30806616	5	21	theme	predominant	753:763	arg1	menaquinone					765:775	the predominant menaquinone	749:775	the predominant menaquinone	749:775	The major fatty acids of strain UJ94T were iso-C15 : 0, summed feature 3 (C16 : 1ω7c/C16 : 1ω6c) and iso-C17 : 0 3-OH; MK-6 was the predominant menaquinone.
30806616	4	22	theme	NaCl	615:618	arg1	presence					591:598	the presence	587:598	the presence of 2-9 % (w/v) NaCl	587:618	Growth of strain UJ94T was observed at 4-37 °C and pH 6.5-8.0 in the presence of 2-9 % (w/v) NaCl.
30806616	3	23	theme	strain	347:352	arg1	UJ94T					354:358	strain UJ94T	347:358	strain UJ94T	347:358	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain UJ94T shared sequence similarities of 98.4, 96.1 and 95.4 % with Tamlana agarivorans JW-26T, Tamlana sedimentorum KMM 9545T and Tamlana crocina HST1-43T, respectively.
30806616	8	24	dep	in	1030:1031	arg1	silico					1033:1038	silico	1033:1038	silico	1033:1038	The ranges of average nucleotide identity and in silico DNA-DNA hybridization estimated by genome-to-genome distance were 90.6-74.2 % and 47.6-14.6 %, respectively, with the type strains of T. agarivorans and T. sedimentorum.
30806616	2	25	theme	rod-shaped	137:146	arg1	strain					190:195	A Gram-stain-negative, aerobic, non-motile, rod-shaped, agarolytic and carrageenolytic bacterial strain	93:195	A Gram-stain-negative, aerobic, non-motile, rod-shaped, agarolytic and carrageenolytic bacterial strain	93:195	A Gram-stain-negative, aerobic, non-motile, rod-shaped, agarolytic and carrageenolytic bacterial strain, designated UJ94T, was isolated from seawater of Uljin in the Republic of Korea.
30806616	5	26	dep	C16 	695:698	arg1	 1ω6c					711:715	 1ω6c	711:715	 1ω6c	711:715	The major fatty acids of strain UJ94T were iso-C15 : 0, summed feature 3 (C16 : 1ω7c/C16 : 1ω6c) and iso-C17 : 0 3-OH; MK-6 was the predominant menaquinone.
30806616	5	26	dep	C16 	695:698	arg1	 1ω7c/C16 					700:709	 1ω7c/C16 	700:709	 1ω7c/C16 	700:709	The major fatty acids of strain UJ94T were iso-C15 : 0, summed feature 3 (C16 : 1ω7c/C16 : 1ω6c) and iso-C17 : 0 3-OH; MK-6 was the predominant menaquinone.
30806616	2	27	from	seawater	234:241	arg1	Republic					259:266	the Republic	255:266	the Republic of Korea	255:275	A Gram-stain-negative, aerobic, non-motile, rod-shaped, agarolytic and carrageenolytic bacterial strain, designated UJ94T, was isolated from seawater of Uljin in the Republic of Korea.
30806616	3	28	theme	%	412:412	arg1	similarities					376:387	sequence similarities	367:387	sequence similarities of 98.4, 96.1 and 95.4 % with Tamlana agarivorans JW-26T, Tamlana sedimentorum KMM 9545T and Tamlana crocina HST1-43T, respectively	367:519	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain UJ94T shared sequence similarities of 98.4, 96.1 and 95.4 % with Tamlana agarivorans JW-26T, Tamlana sedimentorum KMM 9545T and Tamlana crocina HST1-43T, respectively.
30806616	3	29	theme	98.4	392:395	arg1	%					412:412	98.4, 96.1 and 95.4 %	392:412	%	412:412	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain UJ94T shared sequence similarities of 98.4, 96.1 and 95.4 % with Tamlana agarivorans JW-26T, Tamlana sedimentorum KMM 9545T and Tamlana crocina HST1-43T, respectively.
30806616	8	30	theme	nucleotide	1006:1015	arg1	identity					1017:1024	average nucleotide identity	998:1024	average nucleotide identity	998:1024	The ranges of average nucleotide identity and in silico DNA-DNA hybridization estimated by genome-to-genome distance were 90.6-74.2 % and 47.6-14.6 %, respectively, with the type strains of T. agarivorans and T. sedimentorum.
30806616	6	31	theme	unidentified	842:853	arg1	lipids					855:860	five unidentified lipids	837:860	five unidentified lipids	837:860	Phosphatidylethanolamine, two unidentified aminolipids and five unidentified lipids were detected as major polar lipids.
30806616	6	31	theme	unidentified	842:853	arg1	Phosphatidylethanolamine					778:801	Phosphatidylethanolamine	778:801	Phosphatidylethanolamine	778:801	Phosphatidylethanolamine, two unidentified aminolipids and five unidentified lipids were detected as major polar lipids.
30806616	11	32	theme	type	1457:1460	arg1	strain					1462:1467	The type strain	1453:1467	The type strain	1453:1467	The type strain is UJ94T (=KCTC 62451T=NBRC 113234T).
30806616	11	32	theme	type	1457:1460	arg1	UJ94T					1472:1476	UJ94T	1472:1476	UJ94T (=KCTC 62451T=NBRC 113234T)	1472:1504	The type strain is UJ94T (=KCTC 62451T=NBRC 113234T).
30806616	7	33	theme	%	981:981	arg1	content					962:968	a G+C content	956:968	a G+C content of 35.2 mol%	956:981	The whole circular genome comprised 4 116 543 bp and had a G+C content of 35.2 mol%.
30806616	8	34	theme	type	1158:1161	arg1	strains					1163:1169	the type strains	1154:1169	the type strains of T. agarivorans and T. sedimentorum	1154:1207	The ranges of average nucleotide identity and in silico DNA-DNA hybridization estimated by genome-to-genome distance were 90.6-74.2 % and 47.6-14.6 %, respectively, with the type strains of T. agarivorans and T. sedimentorum.
30806616	3	35	theme	sequence	367:374	arg1	similarities					376:387	sequence similarities	367:387	sequence similarities of 98.4, 96.1 and 95.4 % with Tamlana agarivorans JW-26T, Tamlana sedimentorum KMM 9545T and Tamlana crocina HST1-43T, respectively	367:519	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain UJ94T shared sequence similarities of 98.4, 96.1 and 95.4 % with Tamlana agarivorans JW-26T, Tamlana sedimentorum KMM 9545T and Tamlana crocina HST1-43T, respectively.
30806616	4	36	theme	UJ94T	539:543	arg1	Growth					522:527	Growth	522:527	Growth of strain UJ94T	522:543	Growth of strain UJ94T was observed at 4-37 °C and pH 6.5-8.0 in the presence of 2-9 % (w/v) NaCl.
30806616	5	37	theme	strain	646:651	arg1	UJ94T					653:657	strain UJ94T	646:657	strain UJ94T	646:657	The major fatty acids of strain UJ94T were iso-C15 : 0, summed feature 3 (C16 : 1ω7c/C16 : 1ω6c) and iso-C17 : 0 3-OH; MK-6 was the predominant menaquinone.
30806616	9	38	theme	novel	1351:1355	arg1	species					1357:1363	a novel species	1349:1363	a novel species	1349:1363	The present polyphasic study, including phylogenetic, chemotaxonomic, biochemical and genomic data, suggested that strain UJ94T represents a novel species of the genus Tamlana, for which the name Tamlana carrageenivorans sp.
30806616	9	39	theme	Tamlana	1378:1384	arg1	species					1357:1363	a novel species	1349:1363	a novel species	1349:1363	The present polyphasic study, including phylogenetic, chemotaxonomic, biochemical and genomic data, suggested that strain UJ94T represents a novel species of the genus Tamlana, for which the name Tamlana carrageenivorans sp.
30806616	9	40	theme	genomic	1296:1302	arg1	data					1304:1307	phylogenetic, chemotaxonomic, biochemical and genomic data	1250:1307	phylogenetic, chemotaxonomic, biochemical and genomic data	1250:1307	The present polyphasic study, including phylogenetic, chemotaxonomic, biochemical and genomic data, suggested that strain UJ94T represents a novel species of the genus Tamlana, for which the name Tamlana carrageenivorans sp.
30806616	4	41	theme	%	607:607	arg1	NaCl					615:618	2-9 % (w/v) NaCl	603:618	2-9 % (w/v) NaCl	603:618	Growth of strain UJ94T was observed at 4-37 °C and pH 6.5-8.0 in the presence of 2-9 % (w/v) NaCl.
30806616	6	42	theme	major	879:883	arg1	Phosphatidylethanolamine					778:801	Phosphatidylethanolamine	778:801	Phosphatidylethanolamine	778:801	Phosphatidylethanolamine, two unidentified aminolipids and five unidentified lipids were detected as major polar lipids.
30806616	6	42	theme	major	879:883	arg1	lipids					891:896	major polar lipids	879:896	major polar lipids	879:896	Phosphatidylethanolamine, two unidentified aminolipids and five unidentified lipids were detected as major polar lipids.
30806616	3	43	theme	Phylogenetic	278:289	arg1	analysis					291:298	Phylogenetic analysis	278:298	Phylogenetic analysis based on 16S rRNA gene sequences	278:331	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain UJ94T shared sequence similarities of 98.4, 96.1 and 95.4 % with Tamlana agarivorans JW-26T, Tamlana sedimentorum KMM 9545T and Tamlana crocina HST1-43T, respectively.
30806616	8	44	theme	hybridization	1048:1060	arg1	ranges					988:993	The ranges	984:993	The ranges of average nucleotide identity and in silico DNA-DNA hybridization estimated by genome-to-genome distance	984:1099	The ranges of average nucleotide identity and in silico DNA-DNA hybridization estimated by genome-to-genome distance were 90.6-74.2 % and 47.6-14.6 %, respectively, with the type strains of T. agarivorans and T. sedimentorum.
30806616	8	44	theme	hybridization	1048:1060	arg1	%					1116:1116	90.6-74.2 %	1106:1116	90.6-74.2 %	1106:1116	The ranges of average nucleotide identity and in silico DNA-DNA hybridization estimated by genome-to-genome distance were 90.6-74.2 % and 47.6-14.6 %, respectively, with the type strains of T. agarivorans and T. sedimentorum.
30806616	4	45	theme	2-9 	603:606	arg1	%					607:607	%	607:607	%	607:607	Growth of strain UJ94T was observed at 4-37 °C and pH 6.5-8.0 in the presence of 2-9 % (w/v) NaCl.
30806616	7	46	contain	had	952:954	arg2	content					962:968	a G+C content	956:968	a G+C content of 35.2 mol%	956:981	The whole circular genome comprised 4 116 543 bp and had a G+C content of 35.2 mol%.
30806616	7	46	contain	had	952:954	arg1	genome					918:923	The whole circular genome	899:923	The whole circular genome	899:923	The whole circular genome comprised 4 116 543 bp and had a G+C content of 35.2 mol%.
30806616	5	47	dep	feature	684:690	arg1	C16 					695:698	C16 	695:698	C16 	695:698	The major fatty acids of strain UJ94T were iso-C15 : 0, summed feature 3 (C16 : 1ω7c/C16 : 1ω6c) and iso-C17 : 0 3-OH; MK-6 was the predominant menaquinone.
30806616	3	48	theme	95.4 	407:411	arg1	%					412:412	98.4, 96.1 and 95.4 %	392:412	%	412:412	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain UJ94T shared sequence similarities of 98.4, 96.1 and 95.4 % with Tamlana agarivorans JW-26T, Tamlana sedimentorum KMM 9545T and Tamlana crocina HST1-43T, respectively.
30806616	7	49	theme	35.2 mol	973:980	arg1	%					981:981	35.2 mol%	973:981	35.2 mol%	973:981	The whole circular genome comprised 4 116 543 bp and had a G+C content of 35.2 mol%.
30806616	2	50	theme	bacterial	180:188	arg1	strain					190:195	A Gram-stain-negative, aerobic, non-motile, rod-shaped, agarolytic and carrageenolytic bacterial strain	93:195	A Gram-stain-negative, aerobic, non-motile, rod-shaped, agarolytic and carrageenolytic bacterial strain	93:195	A Gram-stain-negative, aerobic, non-motile, rod-shaped, agarolytic and carrageenolytic bacterial strain, designated UJ94T, was isolated from seawater of Uljin in the Republic of Korea.
30806616	9	51	theme	phylogenetic	1250:1261	arg1	data					1304:1307	phylogenetic, chemotaxonomic, biochemical and genomic data	1250:1307	phylogenetic, chemotaxonomic, biochemical and genomic data	1250:1307	The present polyphasic study, including phylogenetic, chemotaxonomic, biochemical and genomic data, suggested that strain UJ94T represents a novel species of the genus Tamlana, for which the name Tamlana carrageenivorans sp.
30806616	11	52	theme	62451T=NBRC	1485:1495	arg1	113234T					1497:1503	=KCTC 62451T=NBRC 113234T	1479:1503	=KCTC 62451T=NBRC 113234T	1479:1503	The type strain is UJ94T (=KCTC 62451T=NBRC 113234T).
30806616	11	52	theme	62451T=NBRC	1485:1495	arg1	UJ94T					1472:1476	UJ94T	1472:1476	UJ94T (=KCTC 62451T=NBRC 113234T)	1472:1504	The type strain is UJ94T (=KCTC 62451T=NBRC 113234T).
30806616	5	53	theme	summed	677:682	arg1	 0					673:674	 0	673:674	 0	673:674	The major fatty acids of strain UJ94T were iso-C15 : 0, summed feature 3 (C16 : 1ω7c/C16 : 1ω6c) and iso-C17 : 0 3-OH; MK-6 was the predominant menaquinone.
30806616	5	53	theme	summed	677:682	arg1	feature					684:690	summed feature 3	677:692	summed feature 3 (C16 : 1ω7c/C16 : 1ω6c)	677:716	The major fatty acids of strain UJ94T were iso-C15 : 0, summed feature 3 (C16 : 1ω7c/C16 : 1ω6c) and iso-C17 : 0 3-OH; MK-6 was the predominant menaquinone.
30806616	2	54	theme	Gram-stain-negative	95:113	arg1	strain					190:195	A Gram-stain-negative, aerobic, non-motile, rod-shaped, agarolytic and carrageenolytic bacterial strain	93:195	A Gram-stain-negative, aerobic, non-motile, rod-shaped, agarolytic and carrageenolytic bacterial strain	93:195	A Gram-stain-negative, aerobic, non-motile, rod-shaped, agarolytic and carrageenolytic bacterial strain, designated UJ94T, was isolated from seawater of Uljin in the Republic of Korea.
30806616	3	55	with	%	412:412	arg1	Tamlana					447:453	Tamlana	447:453	Tamlana	447:453	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain UJ94T shared sequence similarities of 98.4, 96.1 and 95.4 % with Tamlana agarivorans JW-26T, Tamlana sedimentorum KMM 9545T and Tamlana crocina HST1-43T, respectively.
30806616	3	55	with	%	412:412	arg1	9545T					472:476	9545T	472:476	9545T	472:476	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain UJ94T shared sequence similarities of 98.4, 96.1 and 95.4 % with Tamlana agarivorans JW-26T, Tamlana sedimentorum KMM 9545T and Tamlana crocina HST1-43T, respectively.
30806616	2	56	theme	carrageenolytic	164:178	arg1	strain					190:195	A Gram-stain-negative, aerobic, non-motile, rod-shaped, agarolytic and carrageenolytic bacterial strain	93:195	A Gram-stain-negative, aerobic, non-motile, rod-shaped, agarolytic and carrageenolytic bacterial strain	93:195	A Gram-stain-negative, aerobic, non-motile, rod-shaped, agarolytic and carrageenolytic bacterial strain, designated UJ94T, was isolated from seawater of Uljin in the Republic of Korea.
30806616	4	57	dep	%	607:607	arg1	w/v					610:612	w/v	610:612	w/v	610:612	Growth of strain UJ94T was observed at 4-37 °C and pH 6.5-8.0 in the presence of 2-9 % (w/v) NaCl.
30806616	8	58	theme	in	1030:1031	arg1	hybridization					1048:1060	in silico DNA-DNA hybridization	1030:1060	in silico DNA-DNA hybridization	1030:1060	The ranges of average nucleotide identity and in silico DNA-DNA hybridization estimated by genome-to-genome distance were 90.6-74.2 % and 47.6-14.6 %, respectively, with the type strains of T. agarivorans and T. sedimentorum.
30806616	8	59	theme	sedimentorum	1196:1207	arg1	strains					1163:1169	the type strains	1154:1169	the type strains of T. agarivorans and T. sedimentorum	1154:1207	The ranges of average nucleotide identity and in silico DNA-DNA hybridization estimated by genome-to-genome distance were 90.6-74.2 % and 47.6-14.6 %, respectively, with the type strains of T. agarivorans and T. sedimentorum.
30806616	6	60	theme	unidentified	808:819	arg1	aminolipids					821:831	two unidentified aminolipids	804:831	two unidentified aminolipids	804:831	Phosphatidylethanolamine, two unidentified aminolipids and five unidentified lipids were detected as major polar lipids.
30806616	6	60	theme	unidentified	808:819	arg1	Phosphatidylethanolamine					778:801	Phosphatidylethanolamine	778:801	Phosphatidylethanolamine	778:801	Phosphatidylethanolamine, two unidentified aminolipids and five unidentified lipids were detected as major polar lipids.
30806616	4	61	theme	strain	532:537	arg1	UJ94T					539:543	strain UJ94T	532:543	strain UJ94T	532:543	Growth of strain UJ94T was observed at 4-37 °C and pH 6.5-8.0 in the presence of 2-9 % (w/v) NaCl.
30806616	3	62	theme	16S	309:311	arg1	sequences					323:331	16S rRNA gene sequences	309:331	16S rRNA gene sequences	309:331	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain UJ94T shared sequence similarities of 98.4, 96.1 and 95.4 % with Tamlana agarivorans JW-26T, Tamlana sedimentorum KMM 9545T and Tamlana crocina HST1-43T, respectively.
30806616	8	63	with	%	1116:1116	arg1	strains					1163:1169	the type strains	1154:1169	the type strains of T. agarivorans and T. sedimentorum	1154:1207	The ranges of average nucleotide identity and in silico DNA-DNA hybridization estimated by genome-to-genome distance were 90.6-74.2 % and 47.6-14.6 %, respectively, with the type strains of T. agarivorans and T. sedimentorum.
30806616	3	64	theme	Tamlana	482:488	arg1	HST1-43T					498:505	Tamlana crocina HST1-43T	482:505	Tamlana crocina HST1-43T	482:505	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain UJ94T shared sequence similarities of 98.4, 96.1 and 95.4 % with Tamlana agarivorans JW-26T, Tamlana sedimentorum KMM 9545T and Tamlana crocina HST1-43T, respectively.
30806616	2	65	theme	Uljin	246:250	arg1	seawater					234:241	seawater	234:241	seawater of Uljin in the Republic of Korea	234:275	A Gram-stain-negative, aerobic, non-motile, rod-shaped, agarolytic and carrageenolytic bacterial strain, designated UJ94T, was isolated from seawater of Uljin in the Republic of Korea.
30806616	8	66	theme	average	998:1004	arg1	identity					1017:1024	average nucleotide identity	998:1024	average nucleotide identity	998:1024	The ranges of average nucleotide identity and in silico DNA-DNA hybridization estimated by genome-to-genome distance were 90.6-74.2 % and 47.6-14.6 %, respectively, with the type strains of T. agarivorans and T. sedimentorum.
30806616	9	67	theme	chemotaxonomic	1264:1277	arg1	data					1304:1307	phylogenetic, chemotaxonomic, biochemical and genomic data	1250:1307	phylogenetic, chemotaxonomic, biochemical and genomic data	1250:1307	The present polyphasic study, including phylogenetic, chemotaxonomic, biochemical and genomic data, suggested that strain UJ94T represents a novel species of the genus Tamlana, for which the name Tamlana carrageenivorans sp.
30806616	11	68	theme	=KCTC	1479:1483	arg1	113234T					1497:1503	=KCTC 62451T=NBRC 113234T	1479:1503	=KCTC 62451T=NBRC 113234T	1479:1503	The type strain is UJ94T (=KCTC 62451T=NBRC 113234T).
30806616	11	68	theme	=KCTC	1479:1483	arg1	UJ94T					1472:1476	UJ94T	1472:1476	UJ94T (=KCTC 62451T=NBRC 113234T)	1472:1504	The type strain is UJ94T (=KCTC 62451T=NBRC 113234T).
30806616	9	69	theme	name	1401:1404	arg1	Tamlana					1406:1412	the name Tamlana	1397:1412	the name Tamlana	1397:1412	The present polyphasic study, including phylogenetic, chemotaxonomic, biochemical and genomic data, suggested that strain UJ94T represents a novel species of the genus Tamlana, for which the name Tamlana carrageenivorans sp.
30806616	7	70	theme	whole	903:907	arg1	genome					918:923	The whole circular genome	899:923	The whole circular genome	899:923	The whole circular genome comprised 4 116 543 bp and had a G+C content of 35.2 mol%.
30806616	8	71	theme	DNA-DNA	1040:1046	arg1	hybridization					1048:1060	in silico DNA-DNA hybridization	1030:1060	in silico DNA-DNA hybridization	1030:1060	The ranges of average nucleotide identity and in silico DNA-DNA hybridization estimated by genome-to-genome distance were 90.6-74.2 % and 47.6-14.6 %, respectively, with the type strains of T. agarivorans and T. sedimentorum.
30806616	8	72	theme	agarivorans	1177:1187	arg1	strains					1163:1169	the type strains	1154:1169	the type strains of T. agarivorans and T. sedimentorum	1154:1207	The ranges of average nucleotide identity and in silico DNA-DNA hybridization estimated by genome-to-genome distance were 90.6-74.2 % and 47.6-14.6 %, respectively, with the type strains of T. agarivorans and T. sedimentorum.
30806616	7	73	theme	G+C	958:960	arg1	content					962:968	a G+C content	956:968	a G+C content of 35.2 mol%	956:981	The whole circular genome comprised 4 116 543 bp and had a G+C content of 35.2 mol%.
30806616	7	74	theme	circular	909:916	arg1	genome					918:923	The whole circular genome	899:923	The whole circular genome	899:923	The whole circular genome comprised 4 116 543 bp and had a G+C content of 35.2 mol%.
30806616	1	75	theme	carrageenan-degrading	37:57	arg1	bacterium					59:67	a carrageenan-degrading bacterium	35:67	a carrageenan-degrading bacterium	35:67	nov., a carrageenan-degrading bacterium isolated from seawater.
30806616	1	75	theme	carrageenan-degrading	37:57	arg1	nov.					29:32	nov.	29:32	nov.	29:32	nov., a carrageenan-degrading bacterium isolated from seawater.
30806616	9	76	theme	polyphasic	1222:1231	arg1	study					1233:1237	The present polyphasic study	1210:1237	The present polyphasic study	1210:1237	The present polyphasic study, including phylogenetic, chemotaxonomic, biochemical and genomic data, suggested that strain UJ94T represents a novel species of the genus Tamlana, for which the name Tamlana carrageenivorans sp.
30806616	5	77	theme	fatty	631:635	arg1	acids					637:641	The major fatty acids	621:641	The major fatty acids of strain UJ94T	621:657	The major fatty acids of strain UJ94T were iso-C15 : 0, summed feature 3 (C16 : 1ω7c/C16 : 1ω6c) and iso-C17 : 0 3-OH; MK-6 was the predominant menaquinone.
30806616	5	77	theme	fatty	631:635	arg1	iso-C15 					664:671	iso-C15 	664:671	iso-C15 	664:671	The major fatty acids of strain UJ94T were iso-C15 : 0, summed feature 3 (C16 : 1ω7c/C16 : 1ω6c) and iso-C17 : 0 3-OH; MK-6 was the predominant menaquinone.
30806616	9	78	theme	biochemical	1280:1290	arg1	data					1304:1307	phylogenetic, chemotaxonomic, biochemical and genomic data	1250:1307	phylogenetic, chemotaxonomic, biochemical and genomic data	1250:1307	The present polyphasic study, including phylogenetic, chemotaxonomic, biochemical and genomic data, suggested that strain UJ94T represents a novel species of the genus Tamlana, for which the name Tamlana carrageenivorans sp.
30465833	0	0	theme	antibacterial	98:110	arg1	activity					112:119	antibacterial activity	98:119	antibacterial activity	98:119	Physiochemical characterization, antioxidative, anticancer cells proliferation and food pathogens antibacterial activity of chitosan nanoparticles loaded with Cyperus articulatus rhizome essential oils.
30465833	1	1	theme	Scanning	838:845	arg1	Microscopy					856:865	Scanning Electron Microscopy	838:865	Scanning Electron Microscopy (SEM)	838:871	Essential oil of Cyperus articulatus have known bioactivities, the qualitative chemical composition analysis of the Essential Oils (EOs) by Gas Chromatography-Mass Spectrometry (GC-MS) revealed the presence of new compounds not previously known to the plant rhizome which includes, sesquiterpenes, monoterpenes, nootkatone, 6-methyl-3,5-heptadien-2-one, retinene, nopinone, cycloeucalenol, anozol, toosendanin, furanone, ethanone and vitamin A. Cyperus articulatus Essential Oils (CPEOs) loaded Chitosan Nanoparticles (CSNPs) was successful synthesized using an oil-in-water mixture and ionic gelation method and were characterized by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and UV-Vis spectrophotometry.
30465833	1	1	theme	Scanning	838:845	arg1	SEM					868:870	SEM	868:870	SEM	868:870	Essential oil of Cyperus articulatus have known bioactivities, the qualitative chemical composition analysis of the Essential Oils (EOs) by Gas Chromatography-Mass Spectrometry (GC-MS) revealed the presence of new compounds not previously known to the plant rhizome which includes, sesquiterpenes, monoterpenes, nootkatone, 6-methyl-3,5-heptadien-2-one, retinene, nopinone, cycloeucalenol, anozol, toosendanin, furanone, ethanone and vitamin A. Cyperus articulatus Essential Oils (CPEOs) loaded Chitosan Nanoparticles (CSNPs) was successful synthesized using an oil-in-water mixture and ionic gelation method and were characterized by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and UV-Vis spectrophotometry.
30465833	5	2	dep	in	1457:1458	arg1	vitro					1460:1464	vitro	1460:1464	vitro	1460:1464	Trypan blue in vitro exclusion assay showed CPEO-CSNPs to be more cytotoxic against MDA-MB-231 breast cancer cells after 48 h.
30465833	3	3	dep	highest	1080:1086	arg1	P < 0.05					1089:1096	P < 0.05	1089:1096	P < 0.05	1089:1096	CPEO-CSNPs showed the highest (P < 0.05) antioxidant activity over a prolonged period of time.
30465833	1	4	theme	composition	291:301	arg1	analysis					303:310	the qualitative chemical composition analysis	266:310	the qualitative chemical composition analysis of the Essential Oils (EOs) by Gas Chromatography-Mass Spectrometry (GC-MS)	266:386	Essential oil of Cyperus articulatus have known bioactivities, the qualitative chemical composition analysis of the Essential Oils (EOs) by Gas Chromatography-Mass Spectrometry (GC-MS) revealed the presence of new compounds not previously known to the plant rhizome which includes, sesquiterpenes, monoterpenes, nootkatone, 6-methyl-3,5-heptadien-2-one, retinene, nopinone, cycloeucalenol, anozol, toosendanin, furanone, ethanone and vitamin A. Cyperus articulatus Essential Oils (CPEOs) loaded Chitosan Nanoparticles (CSNPs) was successful synthesized using an oil-in-water mixture and ionic gelation method and were characterized by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and UV-Vis spectrophotometry.
30465833	1	5	theme	Electron	847:854	arg1	Microscopy					856:865	Scanning Electron Microscopy	838:865	Scanning Electron Microscopy (SEM)	838:871	Essential oil of Cyperus articulatus have known bioactivities, the qualitative chemical composition analysis of the Essential Oils (EOs) by Gas Chromatography-Mass Spectrometry (GC-MS) revealed the presence of new compounds not previously known to the plant rhizome which includes, sesquiterpenes, monoterpenes, nootkatone, 6-methyl-3,5-heptadien-2-one, retinene, nopinone, cycloeucalenol, anozol, toosendanin, furanone, ethanone and vitamin A. Cyperus articulatus Essential Oils (CPEOs) loaded Chitosan Nanoparticles (CSNPs) was successful synthesized using an oil-in-water mixture and ionic gelation method and were characterized by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and UV-Vis spectrophotometry.
30465833	1	5	theme	Electron	847:854	arg1	SEM					868:870	SEM	868:870	SEM	868:870	Essential oil of Cyperus articulatus have known bioactivities, the qualitative chemical composition analysis of the Essential Oils (EOs) by Gas Chromatography-Mass Spectrometry (GC-MS) revealed the presence of new compounds not previously known to the plant rhizome which includes, sesquiterpenes, monoterpenes, nootkatone, 6-methyl-3,5-heptadien-2-one, retinene, nopinone, cycloeucalenol, anozol, toosendanin, furanone, ethanone and vitamin A. Cyperus articulatus Essential Oils (CPEOs) loaded Chitosan Nanoparticles (CSNPs) was successful synthesized using an oil-in-water mixture and ionic gelation method and were characterized by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and UV-Vis spectrophotometry.
30465833	5	6	theme	Trypan	1445:1450	arg1	assay					1476:1480	Trypan blue in vitro exclusion assay	1445:1480	Trypan blue in vitro exclusion assay	1445:1480	Trypan blue in vitro exclusion assay showed CPEO-CSNPs to be more cytotoxic against MDA-MB-231 breast cancer cells after 48 h.
30465833	1	7	theme	Essential	668:676	arg1	CPEOs					684:688	CPEOs	684:688	CPEOs	684:688	Essential oil of Cyperus articulatus have known bioactivities, the qualitative chemical composition analysis of the Essential Oils (EOs) by Gas Chromatography-Mass Spectrometry (GC-MS) revealed the presence of new compounds not previously known to the plant rhizome which includes, sesquiterpenes, monoterpenes, nootkatone, 6-methyl-3,5-heptadien-2-one, retinene, nopinone, cycloeucalenol, anozol, toosendanin, furanone, ethanone and vitamin A. Cyperus articulatus Essential Oils (CPEOs) loaded Chitosan Nanoparticles (CSNPs) was successful synthesized using an oil-in-water mixture and ionic gelation method and were characterized by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and UV-Vis spectrophotometry.
30465833	1	7	theme	Essential	668:676	arg1	Oils					678:681	sesquiterpenes, monoterpenes, nootkatone, 6-methyl-3,5-heptadien-2-one, retinene, nopinone, cycloeucalenol, anozol, toosendanin, furanone, ethanone and vitamin A. Cyperus articulatus Essential Oils	485:681	sesquiterpenes, monoterpenes, nootkatone, 6-methyl-3,5-heptadien-2-one, retinene, nopinone, cycloeucalenol, anozol, toosendanin, furanone, ethanone and vitamin A. Cyperus articulatus Essential Oils (CPEOs)	485:689	Essential oil of Cyperus articulatus have known bioactivities, the qualitative chemical composition analysis of the Essential Oils (EOs) by Gas Chromatography-Mass Spectrometry (GC-MS) revealed the presence of new compounds not previously known to the plant rhizome which includes, sesquiterpenes, monoterpenes, nootkatone, 6-methyl-3,5-heptadien-2-one, retinene, nopinone, cycloeucalenol, anozol, toosendanin, furanone, ethanone and vitamin A. Cyperus articulatus Essential Oils (CPEOs) loaded Chitosan Nanoparticles (CSNPs) was successful synthesized using an oil-in-water mixture and ionic gelation method and were characterized by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and UV-Vis spectrophotometry.
30465833	4	8	theme	10 mg/mL	1430:1437	arg1	CPEOs					1423:1427	free CPEOs	1418:1427	free CPEOs (10 mg/mL MIC)	1418:1442	Antimicrobial activity of two strains of bacteria, Staphylococcus aureus (ATCC6538), and Escherichia coli (ATCC 8739) showed that all CPEO-loaded chitosan nanoparticle inhibited bacterial growth at lower (P < 0.05) CPEO-CSNP concentration (5 mg/mL MIC) compared to free CPEOs (10 mg/mL MIC).
30465833	4	8	theme	10 mg/mL	1430:1437	arg1	MIC					1439:1441	10 mg/mL MIC	1430:1441	10 mg/mL MIC	1430:1441	Antimicrobial activity of two strains of bacteria, Staphylococcus aureus (ATCC6538), and Escherichia coli (ATCC 8739) showed that all CPEO-loaded chitosan nanoparticle inhibited bacterial growth at lower (P < 0.05) CPEO-CSNP concentration (5 mg/mL MIC) compared to free CPEOs (10 mg/mL MIC).
30465833	6	9	from	applications	1653:1664	arg1	industries					1693:1702	food and pharmaceutical industries	1669:1702	food and pharmaceutical industries	1669:1702	The bioactivity and physiochemical properties of CPEO-CSNPs have shown promising applications in food and pharmaceutical industries.
30465833	4	10	theme	ATCC	1260:1263	arg1	coli					1254:1257	Escherichia coli	1242:1257	Escherichia coli (ATCC 8739)	1242:1269	Antimicrobial activity of two strains of bacteria, Staphylococcus aureus (ATCC6538), and Escherichia coli (ATCC 8739) showed that all CPEO-loaded chitosan nanoparticle inhibited bacterial growth at lower (P < 0.05) CPEO-CSNP concentration (5 mg/mL MIC) compared to free CPEOs (10 mg/mL MIC).
30465833	4	10	theme	ATCC	1260:1263	arg1	8739					1265:1268	ATCC 8739	1260:1268	ATCC 8739	1260:1268	Antimicrobial activity of two strains of bacteria, Staphylococcus aureus (ATCC6538), and Escherichia coli (ATCC 8739) showed that all CPEO-loaded chitosan nanoparticle inhibited bacterial growth at lower (P < 0.05) CPEO-CSNP concentration (5 mg/mL MIC) compared to free CPEOs (10 mg/mL MIC).
30465833	0	11	dep	nanoparticles	133:145	arg1	loaded					147:152	loaded	147:152	nanoparticles loaded with Cyperus articulatus rhizome essential oils	133:200	Physiochemical characterization, antioxidative, anticancer cells proliferation and food pathogens antibacterial activity of chitosan nanoparticles loaded with Cyperus articulatus rhizome essential oils.
30465833	2	12	theme	storage	1043:1049	arg1	times					1051:1055	initial storage times	1035:1055	initial storage times	1035:1055	CPEOs had more radical-scavenging bioactivity in vitro than CSNPs and CPEO-CSNPs at initial storage times.
30465833	3	13	theme	antioxidant	1099:1109	arg1	activity					1111:1118	the highest (P < 0.05) antioxidant activity	1076:1118	the highest (P < 0.05) antioxidant activity	1076:1118	CPEO-CSNPs showed the highest (P < 0.05) antioxidant activity over a prolonged period of time.
30465833	5	14	theme	in	1457:1458	arg1	assay					1476:1480	Trypan blue in vitro exclusion assay	1445:1480	Trypan blue in vitro exclusion assay	1445:1480	Trypan blue in vitro exclusion assay showed CPEO-CSNPs to be more cytotoxic against MDA-MB-231 breast cancer cells after 48 h.
30465833	5	15	theme	MDA-MB-231	1529:1538	arg1	cells					1554:1558	MDA-MB-231 breast cancer cells	1529:1558	MDA-MB-231 breast cancer cells	1529:1558	Trypan blue in vitro exclusion assay showed CPEO-CSNPs to be more cytotoxic against MDA-MB-231 breast cancer cells after 48 h.
30465833	1	16	theme	oil-in-water	765:776	arg1	mixture					778:784	an oil-in-water mixture	762:784	an oil-in-water mixture	762:784	Essential oil of Cyperus articulatus have known bioactivities, the qualitative chemical composition analysis of the Essential Oils (EOs) by Gas Chromatography-Mass Spectrometry (GC-MS) revealed the presence of new compounds not previously known to the plant rhizome which includes, sesquiterpenes, monoterpenes, nootkatone, 6-methyl-3,5-heptadien-2-one, retinene, nopinone, cycloeucalenol, anozol, toosendanin, furanone, ethanone and vitamin A. Cyperus articulatus Essential Oils (CPEOs) loaded Chitosan Nanoparticles (CSNPs) was successful synthesized using an oil-in-water mixture and ionic gelation method and were characterized by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and UV-Vis spectrophotometry.
30465833	2	17	theme	initial	1035:1041	arg1	times					1051:1055	initial storage times	1035:1055	initial storage times	1035:1055	CPEOs had more radical-scavenging bioactivity in vitro than CSNPs and CPEO-CSNPs at initial storage times.
30465833	5	18	theme	exclusion	1466:1474	arg1	assay					1476:1480	Trypan blue in vitro exclusion assay	1445:1480	Trypan blue in vitro exclusion assay	1445:1480	Trypan blue in vitro exclusion assay showed CPEO-CSNPs to be more cytotoxic against MDA-MB-231 breast cancer cells after 48 h.
30465833	6	19	theme	food	1669:1672	arg1	industries					1693:1702	food and pharmaceutical industries	1669:1702	food and pharmaceutical industries	1669:1702	The bioactivity and physiochemical properties of CPEO-CSNPs have shown promising applications in food and pharmaceutical industries.
30465833	6	20	theme	pharmaceutical	1678:1691	arg1	industries					1693:1702	food and pharmaceutical industries	1669:1702	food and pharmaceutical industries	1669:1702	The bioactivity and physiochemical properties of CPEO-CSNPs have shown promising applications in food and pharmaceutical industries.
30465833	1	21	theme	Essential	203:211	arg1	oil					213:215	Essential oil	203:215	Essential oil of Cyperus articulatus	203:238	Essential oil of Cyperus articulatus have known bioactivities, the qualitative chemical composition analysis of the Essential Oils (EOs) by Gas Chromatography-Mass Spectrometry (GC-MS) revealed the presence of new compounds not previously known to the plant rhizome which includes, sesquiterpenes, monoterpenes, nootkatone, 6-methyl-3,5-heptadien-2-one, retinene, nopinone, cycloeucalenol, anozol, toosendanin, furanone, ethanone and vitamin A. Cyperus articulatus Essential Oils (CPEOs) loaded Chitosan Nanoparticles (CSNPs) was successful synthesized using an oil-in-water mixture and ionic gelation method and were characterized by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and UV-Vis spectrophotometry.
30465833	0	22	theme	chitosan	124:131	arg1	pathogens					88:96	food pathogens	83:96	food pathogens	83:96	Physiochemical characterization, antioxidative, anticancer cells proliferation and food pathogens antibacterial activity of chitosan nanoparticles loaded with Cyperus articulatus rhizome essential oils.
30465833	0	22	theme	chitosan	124:131	arg1	characterization					15:30	Physiochemical characterization	0:30	Physiochemical characterization	0:30	Physiochemical characterization, antioxidative, anticancer cells proliferation and food pathogens antibacterial activity of chitosan nanoparticles loaded with Cyperus articulatus rhizome essential oils.
30465833	0	22	theme	chitosan	124:131	arg1	proliferation					65:77	antioxidative, anticancer cells proliferation	33:77	antioxidative, anticancer cells proliferation	33:77	Physiochemical characterization, antioxidative, anticancer cells proliferation and food pathogens antibacterial activity of chitosan nanoparticles loaded with Cyperus articulatus rhizome essential oils.
30465833	3	23	theme	time	1147:1150	arg1	period					1137:1142	a prolonged period	1125:1142	a prolonged period of time	1125:1150	CPEO-CSNPs showed the highest (P < 0.05) antioxidant activity over a prolonged period of time.
30465833	5	24	theme	blue	1452:1455	arg1	assay					1476:1480	Trypan blue in vitro exclusion assay	1445:1480	Trypan blue in vitro exclusion assay	1445:1480	Trypan blue in vitro exclusion assay showed CPEO-CSNPs to be more cytotoxic against MDA-MB-231 breast cancer cells after 48 h.
30465833	2	25	contain	had	957:959	arg1	CPEOs					951:955	CPEOs	951:955	CPEOs	951:955	CPEOs had more radical-scavenging bioactivity in vitro than CSNPs and CPEO-CSNPs at initial storage times.
30465833	2	25	contain	had	957:959	arg2	bioactivity					985:995	more radical-scavenging bioactivity	961:995	more radical-scavenging bioactivity	961:995	CPEOs had more radical-scavenging bioactivity in vitro than CSNPs and CPEO-CSNPs at initial storage times.
30465833	2	26	theme	radical-scavenging	966:983	arg1	bioactivity					985:995	more radical-scavenging bioactivity	961:995	more radical-scavenging bioactivity	961:995	CPEOs had more radical-scavenging bioactivity in vitro than CSNPs and CPEO-CSNPs at initial storage times.
30465833	1	27	dep	Oils	678:681	arg1	loaded					691:696	loaded	691:696	loaded Chitosan Nanoparticles (CSNPs) was successful synthesized using an oil-in-water mixture and ionic gelation method and were characterized by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and UV-Vis spectrophotometry	691:948	Essential oil of Cyperus articulatus have known bioactivities, the qualitative chemical composition analysis of the Essential Oils (EOs) by Gas Chromatography-Mass Spectrometry (GC-MS) revealed the presence of new compounds not previously known to the plant rhizome which includes, sesquiterpenes, monoterpenes, nootkatone, 6-methyl-3,5-heptadien-2-one, retinene, nopinone, cycloeucalenol, anozol, toosendanin, furanone, ethanone and vitamin A. Cyperus articulatus Essential Oils (CPEOs) loaded Chitosan Nanoparticles (CSNPs) was successful synthesized using an oil-in-water mixture and ionic gelation method and were characterized by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and UV-Vis spectrophotometry.
30465833	4	28	theme	strains	1183:1189	arg1	activity					1167:1174	Antimicrobial activity	1153:1174	Antimicrobial activity of two strains of bacteria, Staphylococcus aureus (ATCC6538), and Escherichia coli (ATCC 8739)	1153:1269	Antimicrobial activity of two strains of bacteria, Staphylococcus aureus (ATCC6538), and Escherichia coli (ATCC 8739) showed that all CPEO-loaded chitosan nanoparticle inhibited bacterial growth at lower (P < 0.05) CPEO-CSNP concentration (5 mg/mL MIC) compared to free CPEOs (10 mg/mL MIC).
30465833	0	29	theme	Physiochemical	0:13	arg1	characterization					15:30	Physiochemical characterization	0:30	Physiochemical characterization	0:30	Physiochemical characterization, antioxidative, anticancer cells proliferation and food pathogens antibacterial activity of chitosan nanoparticles loaded with Cyperus articulatus rhizome essential oils.
30465833	1	30	theme	Essential	319:327	arg1	EOs					335:337	EOs	335:337	EOs	335:337	Essential oil of Cyperus articulatus have known bioactivities, the qualitative chemical composition analysis of the Essential Oils (EOs) by Gas Chromatography-Mass Spectrometry (GC-MS) revealed the presence of new compounds not previously known to the plant rhizome which includes, sesquiterpenes, monoterpenes, nootkatone, 6-methyl-3,5-heptadien-2-one, retinene, nopinone, cycloeucalenol, anozol, toosendanin, furanone, ethanone and vitamin A. Cyperus articulatus Essential Oils (CPEOs) loaded Chitosan Nanoparticles (CSNPs) was successful synthesized using an oil-in-water mixture and ionic gelation method and were characterized by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and UV-Vis spectrophotometry.
30465833	1	30	theme	Essential	319:327	arg1	Oils					329:332	the Essential Oils	315:332	the Essential Oils (EOs)	315:338	Essential oil of Cyperus articulatus have known bioactivities, the qualitative chemical composition analysis of the Essential Oils (EOs) by Gas Chromatography-Mass Spectrometry (GC-MS) revealed the presence of new compounds not previously known to the plant rhizome which includes, sesquiterpenes, monoterpenes, nootkatone, 6-methyl-3,5-heptadien-2-one, retinene, nopinone, cycloeucalenol, anozol, toosendanin, furanone, ethanone and vitamin A. Cyperus articulatus Essential Oils (CPEOs) loaded Chitosan Nanoparticles (CSNPs) was successful synthesized using an oil-in-water mixture and ionic gelation method and were characterized by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and UV-Vis spectrophotometry.
30465833	3	31	theme	prolonged	1127:1135	arg1	period					1137:1142	a prolonged period	1125:1142	a prolonged period of time	1125:1150	CPEO-CSNPs showed the highest (P < 0.05) antioxidant activity over a prolonged period of time.
30465833	1	32	dep	Cyperus	648:654	arg1	articulatus					656:666	sesquiterpenes, monoterpenes, nootkatone, 6-methyl-3,5-heptadien-2-one, retinene, nopinone, cycloeucalenol, anozol, toosendanin, furanone, ethanone and vitamin A. Cyperus articulatus	485:666	sesquiterpenes, monoterpenes, nootkatone, 6-methyl-3,5-heptadien-2-one, retinene, nopinone, cycloeucalenol, anozol, toosendanin, furanone, ethanone and vitamin A. Cyperus articulatus Essential Oils (CPEOs)	485:689	Essential oil of Cyperus articulatus have known bioactivities, the qualitative chemical composition analysis of the Essential Oils (EOs) by Gas Chromatography-Mass Spectrometry (GC-MS) revealed the presence of new compounds not previously known to the plant rhizome which includes, sesquiterpenes, monoterpenes, nootkatone, 6-methyl-3,5-heptadien-2-one, retinene, nopinone, cycloeucalenol, anozol, toosendanin, furanone, ethanone and vitamin A. Cyperus articulatus Essential Oils (CPEOs) loaded Chitosan Nanoparticles (CSNPs) was successful synthesized using an oil-in-water mixture and ionic gelation method and were characterized by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and UV-Vis spectrophotometry.
30465833	0	33	theme	antioxidative	33:45	arg1	proliferation					65:77	antioxidative, anticancer cells proliferation	33:77	antioxidative, anticancer cells proliferation	33:77	Physiochemical characterization, antioxidative, anticancer cells proliferation and food pathogens antibacterial activity of chitosan nanoparticles loaded with Cyperus articulatus rhizome essential oils.
30465833	1	34	theme	Oils	329:332	arg1	analysis					303:310	the qualitative chemical composition analysis	266:310	the qualitative chemical composition analysis of the Essential Oils (EOs) by Gas Chromatography-Mass Spectrometry (GC-MS)	266:386	Essential oil of Cyperus articulatus have known bioactivities, the qualitative chemical composition analysis of the Essential Oils (EOs) by Gas Chromatography-Mass Spectrometry (GC-MS) revealed the presence of new compounds not previously known to the plant rhizome which includes, sesquiterpenes, monoterpenes, nootkatone, 6-methyl-3,5-heptadien-2-one, retinene, nopinone, cycloeucalenol, anozol, toosendanin, furanone, ethanone and vitamin A. Cyperus articulatus Essential Oils (CPEOs) loaded Chitosan Nanoparticles (CSNPs) was successful synthesized using an oil-in-water mixture and ionic gelation method and were characterized by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and UV-Vis spectrophotometry.
30465833	1	35	dep	successful	733:742	arg1	synthesized					744:754	synthesized	744:754	synthesized using an oil-in-water mixture and ionic gelation method and were characterized by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and UV-Vis spectrophotometry	744:948	Essential oil of Cyperus articulatus have known bioactivities, the qualitative chemical composition analysis of the Essential Oils (EOs) by Gas Chromatography-Mass Spectrometry (GC-MS) revealed the presence of new compounds not previously known to the plant rhizome which includes, sesquiterpenes, monoterpenes, nootkatone, 6-methyl-3,5-heptadien-2-one, retinene, nopinone, cycloeucalenol, anozol, toosendanin, furanone, ethanone and vitamin A. Cyperus articulatus Essential Oils (CPEOs) loaded Chitosan Nanoparticles (CSNPs) was successful synthesized using an oil-in-water mixture and ionic gelation method and were characterized by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and UV-Vis spectrophotometry.
30465833	3	36	theme	highest	1080:1086	arg1	activity					1111:1118	the highest (P < 0.05) antioxidant activity	1076:1118	the highest (P < 0.05) antioxidant activity	1076:1118	CPEO-CSNPs showed the highest (P < 0.05) antioxidant activity over a prolonged period of time.
30465833	1	37	theme	articulatus	228:238	arg1	oil					213:215	Essential oil	203:215	Essential oil of Cyperus articulatus	203:238	Essential oil of Cyperus articulatus have known bioactivities, the qualitative chemical composition analysis of the Essential Oils (EOs) by Gas Chromatography-Mass Spectrometry (GC-MS) revealed the presence of new compounds not previously known to the plant rhizome which includes, sesquiterpenes, monoterpenes, nootkatone, 6-methyl-3,5-heptadien-2-one, retinene, nopinone, cycloeucalenol, anozol, toosendanin, furanone, ethanone and vitamin A. Cyperus articulatus Essential Oils (CPEOs) loaded Chitosan Nanoparticles (CSNPs) was successful synthesized using an oil-in-water mixture and ionic gelation method and were characterized by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and UV-Vis spectrophotometry.
30465833	4	38	dep	lower	1351:1355	arg1	P < 0.05					1358:1365	P < 0.05	1358:1365	P < 0.05	1358:1365	Antimicrobial activity of two strains of bacteria, Staphylococcus aureus (ATCC6538), and Escherichia coli (ATCC 8739) showed that all CPEO-loaded chitosan nanoparticle inhibited bacterial growth at lower (P < 0.05) CPEO-CSNP concentration (5 mg/mL MIC) compared to free CPEOs (10 mg/mL MIC).
30465833	1	39	theme	ionic	790:794	arg1	method					805:810	ionic gelation method	790:810	ionic gelation method	790:810	Essential oil of Cyperus articulatus have known bioactivities, the qualitative chemical composition analysis of the Essential Oils (EOs) by Gas Chromatography-Mass Spectrometry (GC-MS) revealed the presence of new compounds not previously known to the plant rhizome which includes, sesquiterpenes, monoterpenes, nootkatone, 6-methyl-3,5-heptadien-2-one, retinene, nopinone, cycloeucalenol, anozol, toosendanin, furanone, ethanone and vitamin A. Cyperus articulatus Essential Oils (CPEOs) loaded Chitosan Nanoparticles (CSNPs) was successful synthesized using an oil-in-water mixture and ionic gelation method and were characterized by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and UV-Vis spectrophotometry.
30465833	1	40	theme	new	413:415	arg1	compounds					417:425	new compounds	413:425	new compounds not previously known to the plant rhizome which includes, sesquiterpenes, monoterpenes, nootkatone, 6-methyl-3,5-heptadien-2-one, retinene, nopinone, cycloeucalenol, anozol, toosendanin, furanone, ethanone and vitamin A. Cyperus articulatus Essential Oils (CPEOs) loaded Chitosan Nanoparticles (CSNPs) was successful synthesized using an oil-in-water mixture and ionic gelation method and were characterized by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and UV-Vis spectrophotometry	413:948	Essential oil of Cyperus articulatus have known bioactivities, the qualitative chemical composition analysis of the Essential Oils (EOs) by Gas Chromatography-Mass Spectrometry (GC-MS) revealed the presence of new compounds not previously known to the plant rhizome which includes, sesquiterpenes, monoterpenes, nootkatone, 6-methyl-3,5-heptadien-2-one, retinene, nopinone, cycloeucalenol, anozol, toosendanin, furanone, ethanone and vitamin A. Cyperus articulatus Essential Oils (CPEOs) loaded Chitosan Nanoparticles (CSNPs) was successful synthesized using an oil-in-water mixture and ionic gelation method and were characterized by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and UV-Vis spectrophotometry.
30465833	1	41	theme	Chitosan	698:705	arg1	Nanoparticles					707:719	Chitosan Nanoparticles	698:719	Chitosan Nanoparticles (CSNPs)	698:727	Essential oil of Cyperus articulatus have known bioactivities, the qualitative chemical composition analysis of the Essential Oils (EOs) by Gas Chromatography-Mass Spectrometry (GC-MS) revealed the presence of new compounds not previously known to the plant rhizome which includes, sesquiterpenes, monoterpenes, nootkatone, 6-methyl-3,5-heptadien-2-one, retinene, nopinone, cycloeucalenol, anozol, toosendanin, furanone, ethanone and vitamin A. Cyperus articulatus Essential Oils (CPEOs) loaded Chitosan Nanoparticles (CSNPs) was successful synthesized using an oil-in-water mixture and ionic gelation method and were characterized by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and UV-Vis spectrophotometry.
30465833	1	41	theme	Chitosan	698:705	arg1	CSNPs					722:726	CSNPs	722:726	CSNPs	722:726	Essential oil of Cyperus articulatus have known bioactivities, the qualitative chemical composition analysis of the Essential Oils (EOs) by Gas Chromatography-Mass Spectrometry (GC-MS) revealed the presence of new compounds not previously known to the plant rhizome which includes, sesquiterpenes, monoterpenes, nootkatone, 6-methyl-3,5-heptadien-2-one, retinene, nopinone, cycloeucalenol, anozol, toosendanin, furanone, ethanone and vitamin A. Cyperus articulatus Essential Oils (CPEOs) loaded Chitosan Nanoparticles (CSNPs) was successful synthesized using an oil-in-water mixture and ionic gelation method and were characterized by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and UV-Vis spectrophotometry.
30465833	1	42	theme	gelation	796:803	arg1	method					805:810	ionic gelation method	790:810	ionic gelation method	790:810	Essential oil of Cyperus articulatus have known bioactivities, the qualitative chemical composition analysis of the Essential Oils (EOs) by Gas Chromatography-Mass Spectrometry (GC-MS) revealed the presence of new compounds not previously known to the plant rhizome which includes, sesquiterpenes, monoterpenes, nootkatone, 6-methyl-3,5-heptadien-2-one, retinene, nopinone, cycloeucalenol, anozol, toosendanin, furanone, ethanone and vitamin A. Cyperus articulatus Essential Oils (CPEOs) loaded Chitosan Nanoparticles (CSNPs) was successful synthesized using an oil-in-water mixture and ionic gelation method and were characterized by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and UV-Vis spectrophotometry.
30465833	1	43	theme	compounds	417:425	arg1	presence					401:408	the presence	397:408	the presence of new compounds not previously known to the plant rhizome which includes, sesquiterpenes, monoterpenes, nootkatone, 6-methyl-3,5-heptadien-2-one, retinene, nopinone, cycloeucalenol, anozol, toosendanin, furanone, ethanone and vitamin A. Cyperus articulatus Essential Oils (CPEOs) loaded Chitosan Nanoparticles (CSNPs) was successful synthesized using an oil-in-water mixture and ionic gelation method and were characterized by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and UV-Vis spectrophotometry	397:948	Essential oil of Cyperus articulatus have known bioactivities, the qualitative chemical composition analysis of the Essential Oils (EOs) by Gas Chromatography-Mass Spectrometry (GC-MS) revealed the presence of new compounds not previously known to the plant rhizome which includes, sesquiterpenes, monoterpenes, nootkatone, 6-methyl-3,5-heptadien-2-one, retinene, nopinone, cycloeucalenol, anozol, toosendanin, furanone, ethanone and vitamin A. Cyperus articulatus Essential Oils (CPEOs) loaded Chitosan Nanoparticles (CSNPs) was successful synthesized using an oil-in-water mixture and ionic gelation method and were characterized by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and UV-Vis spectrophotometry.
30465833	4	44	theme	Antimicrobial	1153:1165	arg1	activity					1167:1174	Antimicrobial activity	1153:1174	Antimicrobial activity of two strains of bacteria, Staphylococcus aureus (ATCC6538), and Escherichia coli (ATCC 8739)	1153:1269	Antimicrobial activity of two strains of bacteria, Staphylococcus aureus (ATCC6538), and Escherichia coli (ATCC 8739) showed that all CPEO-loaded chitosan nanoparticle inhibited bacterial growth at lower (P < 0.05) CPEO-CSNP concentration (5 mg/mL MIC) compared to free CPEOs (10 mg/mL MIC).
30465833	1	45	theme	known	245:249	arg1	bioactivities					251:263	known bioactivities	245:263	known bioactivities	245:263	Essential oil of Cyperus articulatus have known bioactivities, the qualitative chemical composition analysis of the Essential Oils (EOs) by Gas Chromatography-Mass Spectrometry (GC-MS) revealed the presence of new compounds not previously known to the plant rhizome which includes, sesquiterpenes, monoterpenes, nootkatone, 6-methyl-3,5-heptadien-2-one, retinene, nopinone, cycloeucalenol, anozol, toosendanin, furanone, ethanone and vitamin A. Cyperus articulatus Essential Oils (CPEOs) loaded Chitosan Nanoparticles (CSNPs) was successful synthesized using an oil-in-water mixture and ionic gelation method and were characterized by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and UV-Vis spectrophotometry.
30465833	4	46	theme	chitosan	1299:1306	arg1	nanoparticle					1308:1319	all CPEO-loaded chitosan nanoparticle	1283:1319	all CPEO-loaded chitosan nanoparticle	1283:1319	Antimicrobial activity of two strains of bacteria, Staphylococcus aureus (ATCC6538), and Escherichia coli (ATCC 8739) showed that all CPEO-loaded chitosan nanoparticle inhibited bacterial growth at lower (P < 0.05) CPEO-CSNP concentration (5 mg/mL MIC) compared to free CPEOs (10 mg/mL MIC).
30465833	0	47	theme	Cyperus	159:165	arg1	oils					197:200	Cyperus articulatus rhizome essential oils	159:200	Cyperus articulatus rhizome essential oils	159:200	Physiochemical characterization, antioxidative, anticancer cells proliferation and food pathogens antibacterial activity of chitosan nanoparticles loaded with Cyperus articulatus rhizome essential oils.
30465833	1	48	theme	Infrared	892:899	arg1	Spectroscopy					901:912	Infrared Spectroscopy	892:912	Infrared Spectroscopy (FTIR)	892:919	Essential oil of Cyperus articulatus have known bioactivities, the qualitative chemical composition analysis of the Essential Oils (EOs) by Gas Chromatography-Mass Spectrometry (GC-MS) revealed the presence of new compounds not previously known to the plant rhizome which includes, sesquiterpenes, monoterpenes, nootkatone, 6-methyl-3,5-heptadien-2-one, retinene, nopinone, cycloeucalenol, anozol, toosendanin, furanone, ethanone and vitamin A. Cyperus articulatus Essential Oils (CPEOs) loaded Chitosan Nanoparticles (CSNPs) was successful synthesized using an oil-in-water mixture and ionic gelation method and were characterized by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and UV-Vis spectrophotometry.
30465833	1	48	theme	Infrared	892:899	arg1	FTIR					915:918	FTIR	915:918	FTIR	915:918	Essential oil of Cyperus articulatus have known bioactivities, the qualitative chemical composition analysis of the Essential Oils (EOs) by Gas Chromatography-Mass Spectrometry (GC-MS) revealed the presence of new compounds not previously known to the plant rhizome which includes, sesquiterpenes, monoterpenes, nootkatone, 6-methyl-3,5-heptadien-2-one, retinene, nopinone, cycloeucalenol, anozol, toosendanin, furanone, ethanone and vitamin A. Cyperus articulatus Essential Oils (CPEOs) loaded Chitosan Nanoparticles (CSNPs) was successful synthesized using an oil-in-water mixture and ionic gelation method and were characterized by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and UV-Vis spectrophotometry.
30465833	4	49	theme	free	1418:1421	arg1	CPEOs					1423:1427	free CPEOs	1418:1427	free CPEOs (10 mg/mL MIC)	1418:1442	Antimicrobial activity of two strains of bacteria, Staphylococcus aureus (ATCC6538), and Escherichia coli (ATCC 8739) showed that all CPEO-loaded chitosan nanoparticle inhibited bacterial growth at lower (P < 0.05) CPEO-CSNP concentration (5 mg/mL MIC) compared to free CPEOs (10 mg/mL MIC).
30465833	4	49	theme	free	1418:1421	arg1	MIC					1439:1441	10 mg/mL MIC	1430:1441	10 mg/mL MIC	1430:1441	Antimicrobial activity of two strains of bacteria, Staphylococcus aureus (ATCC6538), and Escherichia coli (ATCC 8739) showed that all CPEO-loaded chitosan nanoparticle inhibited bacterial growth at lower (P < 0.05) CPEO-CSNP concentration (5 mg/mL MIC) compared to free CPEOs (10 mg/mL MIC).
30465833	4	50	theme	5 mg/mL	1393:1399	arg1	concentration					1378:1390	lower (P < 0.05) CPEO-CSNP concentration	1351:1390	lower (P < 0.05) CPEO-CSNP concentration (5 mg/mL MIC)	1351:1404	Antimicrobial activity of two strains of bacteria, Staphylococcus aureus (ATCC6538), and Escherichia coli (ATCC 8739) showed that all CPEO-loaded chitosan nanoparticle inhibited bacterial growth at lower (P < 0.05) CPEO-CSNP concentration (5 mg/mL MIC) compared to free CPEOs (10 mg/mL MIC).
30465833	4	50	theme	5 mg/mL	1393:1399	arg1	MIC					1401:1403	5 mg/mL MIC	1393:1403	5 mg/mL MIC	1393:1403	Antimicrobial activity of two strains of bacteria, Staphylococcus aureus (ATCC6538), and Escherichia coli (ATCC 8739) showed that all CPEO-loaded chitosan nanoparticle inhibited bacterial growth at lower (P < 0.05) CPEO-CSNP concentration (5 mg/mL MIC) compared to free CPEOs (10 mg/mL MIC).
30465833	0	51	theme	essential	187:195	arg1	oils					197:200	Cyperus articulatus rhizome essential oils	159:200	Cyperus articulatus rhizome essential oils	159:200	Physiochemical characterization, antioxidative, anticancer cells proliferation and food pathogens antibacterial activity of chitosan nanoparticles loaded with Cyperus articulatus rhizome essential oils.
30465833	4	52	theme	bacterial	1331:1339	arg1	growth					1341:1346	bacterial growth	1331:1346	bacterial growth	1331:1346	Antimicrobial activity of two strains of bacteria, Staphylococcus aureus (ATCC6538), and Escherichia coli (ATCC 8739) showed that all CPEO-loaded chitosan nanoparticle inhibited bacterial growth at lower (P < 0.05) CPEO-CSNP concentration (5 mg/mL MIC) compared to free CPEOs (10 mg/mL MIC).
30465833	6	53	theme	CPEO-CSNPs	1621:1630	arg1	properties					1607:1616	The bioactivity and physiochemical properties	1572:1616	The bioactivity and physiochemical properties of CPEO-CSNPs	1572:1630	The bioactivity and physiochemical properties of CPEO-CSNPs have shown promising applications in food and pharmaceutical industries.
30465833	0	54	theme	cells	59:63	arg1	proliferation					65:77	antioxidative, anticancer cells proliferation	33:77	antioxidative, anticancer cells proliferation	33:77	Physiochemical characterization, antioxidative, anticancer cells proliferation and food pathogens antibacterial activity of chitosan nanoparticles loaded with Cyperus articulatus rhizome essential oils.
30465833	1	55	theme	Gas	343:345	arg1	GC-MS					381:385	GC-MS	381:385	GC-MS	381:385	Essential oil of Cyperus articulatus have known bioactivities, the qualitative chemical composition analysis of the Essential Oils (EOs) by Gas Chromatography-Mass Spectrometry (GC-MS) revealed the presence of new compounds not previously known to the plant rhizome which includes, sesquiterpenes, monoterpenes, nootkatone, 6-methyl-3,5-heptadien-2-one, retinene, nopinone, cycloeucalenol, anozol, toosendanin, furanone, ethanone and vitamin A. Cyperus articulatus Essential Oils (CPEOs) loaded Chitosan Nanoparticles (CSNPs) was successful synthesized using an oil-in-water mixture and ionic gelation method and were characterized by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and UV-Vis spectrophotometry.
30465833	1	55	theme	Gas	343:345	arg1	Spectrometry					367:378	Gas Chromatography-Mass Spectrometry	343:378	Gas Chromatography-Mass Spectrometry (GC-MS)	343:386	Essential oil of Cyperus articulatus have known bioactivities, the qualitative chemical composition analysis of the Essential Oils (EOs) by Gas Chromatography-Mass Spectrometry (GC-MS) revealed the presence of new compounds not previously known to the plant rhizome which includes, sesquiterpenes, monoterpenes, nootkatone, 6-methyl-3,5-heptadien-2-one, retinene, nopinone, cycloeucalenol, anozol, toosendanin, furanone, ethanone and vitamin A. Cyperus articulatus Essential Oils (CPEOs) loaded Chitosan Nanoparticles (CSNPs) was successful synthesized using an oil-in-water mixture and ionic gelation method and were characterized by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and UV-Vis spectrophotometry.
30465833	0	56	theme	rhizome	179:185	arg1	oils					197:200	Cyperus articulatus rhizome essential oils	159:200	Cyperus articulatus rhizome essential oils	159:200	Physiochemical characterization, antioxidative, anticancer cells proliferation and food pathogens antibacterial activity of chitosan nanoparticles loaded with Cyperus articulatus rhizome essential oils.
30465833	1	57	dep	includes	475:482	arg1	CPEOs					684:688	CPEOs	684:688	CPEOs	684:688	Essential oil of Cyperus articulatus have known bioactivities, the qualitative chemical composition analysis of the Essential Oils (EOs) by Gas Chromatography-Mass Spectrometry (GC-MS) revealed the presence of new compounds not previously known to the plant rhizome which includes, sesquiterpenes, monoterpenes, nootkatone, 6-methyl-3,5-heptadien-2-one, retinene, nopinone, cycloeucalenol, anozol, toosendanin, furanone, ethanone and vitamin A. Cyperus articulatus Essential Oils (CPEOs) loaded Chitosan Nanoparticles (CSNPs) was successful synthesized using an oil-in-water mixture and ionic gelation method and were characterized by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and UV-Vis spectrophotometry.
30465833	1	57	dep	includes	475:482	arg1	Oils					678:681	sesquiterpenes, monoterpenes, nootkatone, 6-methyl-3,5-heptadien-2-one, retinene, nopinone, cycloeucalenol, anozol, toosendanin, furanone, ethanone and vitamin A. Cyperus articulatus Essential Oils	485:681	sesquiterpenes, monoterpenes, nootkatone, 6-methyl-3,5-heptadien-2-one, retinene, nopinone, cycloeucalenol, anozol, toosendanin, furanone, ethanone and vitamin A. Cyperus articulatus Essential Oils (CPEOs)	485:689	Essential oil of Cyperus articulatus have known bioactivities, the qualitative chemical composition analysis of the Essential Oils (EOs) by Gas Chromatography-Mass Spectrometry (GC-MS) revealed the presence of new compounds not previously known to the plant rhizome which includes, sesquiterpenes, monoterpenes, nootkatone, 6-methyl-3,5-heptadien-2-one, retinene, nopinone, cycloeucalenol, anozol, toosendanin, furanone, ethanone and vitamin A. Cyperus articulatus Essential Oils (CPEOs) loaded Chitosan Nanoparticles (CSNPs) was successful synthesized using an oil-in-water mixture and ionic gelation method and were characterized by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and UV-Vis spectrophotometry.
30465833	1	58	theme	Chromatography-Mass	347:365	arg1	GC-MS					381:385	GC-MS	381:385	GC-MS	381:385	Essential oil of Cyperus articulatus have known bioactivities, the qualitative chemical composition analysis of the Essential Oils (EOs) by Gas Chromatography-Mass Spectrometry (GC-MS) revealed the presence of new compounds not previously known to the plant rhizome which includes, sesquiterpenes, monoterpenes, nootkatone, 6-methyl-3,5-heptadien-2-one, retinene, nopinone, cycloeucalenol, anozol, toosendanin, furanone, ethanone and vitamin A. Cyperus articulatus Essential Oils (CPEOs) loaded Chitosan Nanoparticles (CSNPs) was successful synthesized using an oil-in-water mixture and ionic gelation method and were characterized by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and UV-Vis spectrophotometry.
30465833	1	58	theme	Chromatography-Mass	347:365	arg1	Spectrometry					367:378	Gas Chromatography-Mass Spectrometry	343:378	Gas Chromatography-Mass Spectrometry (GC-MS)	343:386	Essential oil of Cyperus articulatus have known bioactivities, the qualitative chemical composition analysis of the Essential Oils (EOs) by Gas Chromatography-Mass Spectrometry (GC-MS) revealed the presence of new compounds not previously known to the plant rhizome which includes, sesquiterpenes, monoterpenes, nootkatone, 6-methyl-3,5-heptadien-2-one, retinene, nopinone, cycloeucalenol, anozol, toosendanin, furanone, ethanone and vitamin A. Cyperus articulatus Essential Oils (CPEOs) loaded Chitosan Nanoparticles (CSNPs) was successful synthesized using an oil-in-water mixture and ionic gelation method and were characterized by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and UV-Vis spectrophotometry.
30465833	5	59	theme	breast	1540:1545	arg1	cells					1554:1558	MDA-MB-231 breast cancer cells	1529:1558	MDA-MB-231 breast cancer cells	1529:1558	Trypan blue in vitro exclusion assay showed CPEO-CSNPs to be more cytotoxic against MDA-MB-231 breast cancer cells after 48 h.
30465833	0	60	dep	antioxidative	33:45	arg1	anticancer					48:57	anticancer	48:57	anticancer	48:57	Physiochemical characterization, antioxidative, anticancer cells proliferation and food pathogens antibacterial activity of chitosan nanoparticles loaded with Cyperus articulatus rhizome essential oils.
30465833	4	61	theme	CPEO-loaded	1287:1297	arg1	nanoparticle					1308:1319	all CPEO-loaded chitosan nanoparticle	1283:1319	all CPEO-loaded chitosan nanoparticle	1283:1319	Antimicrobial activity of two strains of bacteria, Staphylococcus aureus (ATCC6538), and Escherichia coli (ATCC 8739) showed that all CPEO-loaded chitosan nanoparticle inhibited bacterial growth at lower (P < 0.05) CPEO-CSNP concentration (5 mg/mL MIC) compared to free CPEOs (10 mg/mL MIC).
30465833	4	62	theme	lower	1351:1355	arg1	concentration					1378:1390	lower (P < 0.05) CPEO-CSNP concentration	1351:1390	lower (P < 0.05) CPEO-CSNP concentration (5 mg/mL MIC)	1351:1404	Antimicrobial activity of two strains of bacteria, Staphylococcus aureus (ATCC6538), and Escherichia coli (ATCC 8739) showed that all CPEO-loaded chitosan nanoparticle inhibited bacterial growth at lower (P < 0.05) CPEO-CSNP concentration (5 mg/mL MIC) compared to free CPEOs (10 mg/mL MIC).
30465833	4	62	theme	lower	1351:1355	arg1	MIC					1401:1403	5 mg/mL MIC	1393:1403	5 mg/mL MIC	1393:1403	Antimicrobial activity of two strains of bacteria, Staphylococcus aureus (ATCC6538), and Escherichia coli (ATCC 8739) showed that all CPEO-loaded chitosan nanoparticle inhibited bacterial growth at lower (P < 0.05) CPEO-CSNP concentration (5 mg/mL MIC) compared to free CPEOs (10 mg/mL MIC).
30465833	5	63	theme	cancer	1547:1552	arg1	cells					1554:1558	MDA-MB-231 breast cancer cells	1529:1558	MDA-MB-231 breast cancer cells	1529:1558	Trypan blue in vitro exclusion assay showed CPEO-CSNPs to be more cytotoxic against MDA-MB-231 breast cancer cells after 48 h.
30465833	1	64	contain	have	240:243	arg2	bioactivities					251:263	known bioactivities	245:263	known bioactivities	245:263	Essential oil of Cyperus articulatus have known bioactivities, the qualitative chemical composition analysis of the Essential Oils (EOs) by Gas Chromatography-Mass Spectrometry (GC-MS) revealed the presence of new compounds not previously known to the plant rhizome which includes, sesquiterpenes, monoterpenes, nootkatone, 6-methyl-3,5-heptadien-2-one, retinene, nopinone, cycloeucalenol, anozol, toosendanin, furanone, ethanone and vitamin A. Cyperus articulatus Essential Oils (CPEOs) loaded Chitosan Nanoparticles (CSNPs) was successful synthesized using an oil-in-water mixture and ionic gelation method and were characterized by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and UV-Vis spectrophotometry.
30465833	1	64	contain	have	240:243	arg1	oil					213:215	Essential oil	203:215	Essential oil of Cyperus articulatus	203:238	Essential oil of Cyperus articulatus have known bioactivities, the qualitative chemical composition analysis of the Essential Oils (EOs) by Gas Chromatography-Mass Spectrometry (GC-MS) revealed the presence of new compounds not previously known to the plant rhizome which includes, sesquiterpenes, monoterpenes, nootkatone, 6-methyl-3,5-heptadien-2-one, retinene, nopinone, cycloeucalenol, anozol, toosendanin, furanone, ethanone and vitamin A. Cyperus articulatus Essential Oils (CPEOs) loaded Chitosan Nanoparticles (CSNPs) was successful synthesized using an oil-in-water mixture and ionic gelation method and were characterized by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and UV-Vis spectrophotometry.
30465833	1	65	dep	bioactivities	251:263	arg1	revealed					388:395	revealed	388:395	revealed the presence of new compounds not previously known to the plant rhizome which includes, sesquiterpenes, monoterpenes, nootkatone, 6-methyl-3,5-heptadien-2-one, retinene, nopinone, cycloeucalenol, anozol, toosendanin, furanone, ethanone and vitamin A. Cyperus articulatus Essential Oils (CPEOs) loaded Chitosan Nanoparticles (CSNPs) was successful synthesized using an oil-in-water mixture and ionic gelation method and were characterized by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and UV-Vis spectrophotometry	388:948	Essential oil of Cyperus articulatus have known bioactivities, the qualitative chemical composition analysis of the Essential Oils (EOs) by Gas Chromatography-Mass Spectrometry (GC-MS) revealed the presence of new compounds not previously known to the plant rhizome which includes, sesquiterpenes, monoterpenes, nootkatone, 6-methyl-3,5-heptadien-2-one, retinene, nopinone, cycloeucalenol, anozol, toosendanin, furanone, ethanone and vitamin A. Cyperus articulatus Essential Oils (CPEOs) loaded Chitosan Nanoparticles (CSNPs) was successful synthesized using an oil-in-water mixture and ionic gelation method and were characterized by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and UV-Vis spectrophotometry.
30465833	6	66	theme	physiochemical	1592:1605	arg1	properties					1607:1616	The bioactivity and physiochemical properties	1572:1616	The bioactivity and physiochemical properties of CPEO-CSNPs	1572:1630	The bioactivity and physiochemical properties of CPEO-CSNPs have shown promising applications in food and pharmaceutical industries.
30465833	0	67	theme	food	83:86	arg1	pathogens					88:96	food pathogens	83:96	food pathogens	83:96	Physiochemical characterization, antioxidative, anticancer cells proliferation and food pathogens antibacterial activity of chitosan nanoparticles loaded with Cyperus articulatus rhizome essential oils.
30465833	1	68	theme	plant	455:459	arg1	rhizome					461:467	the plant rhizome	451:467	the plant rhizome which includes, sesquiterpenes, monoterpenes, nootkatone, 6-methyl-3,5-heptadien-2-one, retinene, nopinone, cycloeucalenol, anozol, toosendanin, furanone, ethanone and vitamin A. Cyperus articulatus Essential Oils (CPEOs) loaded Chitosan Nanoparticles (CSNPs) was successful synthesized using an oil-in-water mixture and ionic gelation method and were characterized by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and UV-Vis spectrophotometry	451:948	Essential oil of Cyperus articulatus have known bioactivities, the qualitative chemical composition analysis of the Essential Oils (EOs) by Gas Chromatography-Mass Spectrometry (GC-MS) revealed the presence of new compounds not previously known to the plant rhizome which includes, sesquiterpenes, monoterpenes, nootkatone, 6-methyl-3,5-heptadien-2-one, retinene, nopinone, cycloeucalenol, anozol, toosendanin, furanone, ethanone and vitamin A. Cyperus articulatus Essential Oils (CPEOs) loaded Chitosan Nanoparticles (CSNPs) was successful synthesized using an oil-in-water mixture and ionic gelation method and were characterized by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and UV-Vis spectrophotometry.
30465833	0	69	dep	characterization	15:30	arg1	activity					112:119	antibacterial activity	98:119	antibacterial activity	98:119	Physiochemical characterization, antioxidative, anticancer cells proliferation and food pathogens antibacterial activity of chitosan nanoparticles loaded with Cyperus articulatus rhizome essential oils.
30465833	4	70	theme	bacteria	1194:1201	arg1	coli					1254:1257	Escherichia coli	1242:1257	Escherichia coli (ATCC 8739)	1242:1269	Antimicrobial activity of two strains of bacteria, Staphylococcus aureus (ATCC6538), and Escherichia coli (ATCC 8739) showed that all CPEO-loaded chitosan nanoparticle inhibited bacterial growth at lower (P < 0.05) CPEO-CSNP concentration (5 mg/mL MIC) compared to free CPEOs (10 mg/mL MIC).
30465833	4	70	theme	bacteria	1194:1201	arg1	aureus					1219:1224	Staphylococcus aureus	1204:1224	Staphylococcus aureus (ATCC6538)	1204:1235	Antimicrobial activity of two strains of bacteria, Staphylococcus aureus (ATCC6538), and Escherichia coli (ATCC 8739) showed that all CPEO-loaded chitosan nanoparticle inhibited bacterial growth at lower (P < 0.05) CPEO-CSNP concentration (5 mg/mL MIC) compared to free CPEOs (10 mg/mL MIC).
30465833	4	70	theme	bacteria	1194:1201	arg1	strains					1183:1189	two strains	1179:1189	two strains of bacteria	1179:1201	Antimicrobial activity of two strains of bacteria, Staphylococcus aureus (ATCC6538), and Escherichia coli (ATCC 8739) showed that all CPEO-loaded chitosan nanoparticle inhibited bacterial growth at lower (P < 0.05) CPEO-CSNP concentration (5 mg/mL MIC) compared to free CPEOs (10 mg/mL MIC).
30465833	6	71	theme	promising	1643:1651	arg1	applications					1653:1664	promising applications	1643:1664	promising applications in food and pharmaceutical industries	1643:1702	The bioactivity and physiochemical properties of CPEO-CSNPs have shown promising applications in food and pharmaceutical industries.
30465833	1	72	theme	qualitative	270:280	arg1	analysis					303:310	the qualitative chemical composition analysis	266:310	the qualitative chemical composition analysis of the Essential Oils (EOs) by Gas Chromatography-Mass Spectrometry (GC-MS)	266:386	Essential oil of Cyperus articulatus have known bioactivities, the qualitative chemical composition analysis of the Essential Oils (EOs) by Gas Chromatography-Mass Spectrometry (GC-MS) revealed the presence of new compounds not previously known to the plant rhizome which includes, sesquiterpenes, monoterpenes, nootkatone, 6-methyl-3,5-heptadien-2-one, retinene, nopinone, cycloeucalenol, anozol, toosendanin, furanone, ethanone and vitamin A. Cyperus articulatus Essential Oils (CPEOs) loaded Chitosan Nanoparticles (CSNPs) was successful synthesized using an oil-in-water mixture and ionic gelation method and were characterized by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and UV-Vis spectrophotometry.
30465833	4	73	theme	CPEO-CSNP	1368:1376	arg1	concentration					1378:1390	lower (P < 0.05) CPEO-CSNP concentration	1351:1390	lower (P < 0.05) CPEO-CSNP concentration (5 mg/mL MIC)	1351:1404	Antimicrobial activity of two strains of bacteria, Staphylococcus aureus (ATCC6538), and Escherichia coli (ATCC 8739) showed that all CPEO-loaded chitosan nanoparticle inhibited bacterial growth at lower (P < 0.05) CPEO-CSNP concentration (5 mg/mL MIC) compared to free CPEOs (10 mg/mL MIC).
30465833	4	73	theme	CPEO-CSNP	1368:1376	arg1	MIC					1401:1403	5 mg/mL MIC	1393:1403	5 mg/mL MIC	1393:1403	Antimicrobial activity of two strains of bacteria, Staphylococcus aureus (ATCC6538), and Escherichia coli (ATCC 8739) showed that all CPEO-loaded chitosan nanoparticle inhibited bacterial growth at lower (P < 0.05) CPEO-CSNP concentration (5 mg/mL MIC) compared to free CPEOs (10 mg/mL MIC).
30465833	1	74	theme	UV-Vis	925:930	arg1	spectrophotometry					932:948	UV-Vis spectrophotometry	925:948	UV-Vis spectrophotometry	925:948	Essential oil of Cyperus articulatus have known bioactivities, the qualitative chemical composition analysis of the Essential Oils (EOs) by Gas Chromatography-Mass Spectrometry (GC-MS) revealed the presence of new compounds not previously known to the plant rhizome which includes, sesquiterpenes, monoterpenes, nootkatone, 6-methyl-3,5-heptadien-2-one, retinene, nopinone, cycloeucalenol, anozol, toosendanin, furanone, ethanone and vitamin A. Cyperus articulatus Essential Oils (CPEOs) loaded Chitosan Nanoparticles (CSNPs) was successful synthesized using an oil-in-water mixture and ionic gelation method and were characterized by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and UV-Vis spectrophotometry.
30465833	6	75	theme	bioactivity	1576:1586	arg1	properties					1607:1616	The bioactivity and physiochemical properties	1572:1616	The bioactivity and physiochemical properties of CPEO-CSNPs	1572:1630	The bioactivity and physiochemical properties of CPEO-CSNPs have shown promising applications in food and pharmaceutical industries.
30465833	0	76	dep	Cyperus	159:165	arg1	articulatus					167:177	articulatus	167:177	articulatus	167:177	Physiochemical characterization, antioxidative, anticancer cells proliferation and food pathogens antibacterial activity of chitosan nanoparticles loaded with Cyperus articulatus rhizome essential oils.
30465833	1	77	theme	Cyperus	648:654	arg1	CPEOs					684:688	CPEOs	684:688	CPEOs	684:688	Essential oil of Cyperus articulatus have known bioactivities, the qualitative chemical composition analysis of the Essential Oils (EOs) by Gas Chromatography-Mass Spectrometry (GC-MS) revealed the presence of new compounds not previously known to the plant rhizome which includes, sesquiterpenes, monoterpenes, nootkatone, 6-methyl-3,5-heptadien-2-one, retinene, nopinone, cycloeucalenol, anozol, toosendanin, furanone, ethanone and vitamin A. Cyperus articulatus Essential Oils (CPEOs) loaded Chitosan Nanoparticles (CSNPs) was successful synthesized using an oil-in-water mixture and ionic gelation method and were characterized by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and UV-Vis spectrophotometry.
30465833	1	77	theme	Cyperus	648:654	arg1	Oils					678:681	sesquiterpenes, monoterpenes, nootkatone, 6-methyl-3,5-heptadien-2-one, retinene, nopinone, cycloeucalenol, anozol, toosendanin, furanone, ethanone and vitamin A. Cyperus articulatus Essential Oils	485:681	sesquiterpenes, monoterpenes, nootkatone, 6-methyl-3,5-heptadien-2-one, retinene, nopinone, cycloeucalenol, anozol, toosendanin, furanone, ethanone and vitamin A. Cyperus articulatus Essential Oils (CPEOs)	485:689	Essential oil of Cyperus articulatus have known bioactivities, the qualitative chemical composition analysis of the Essential Oils (EOs) by Gas Chromatography-Mass Spectrometry (GC-MS) revealed the presence of new compounds not previously known to the plant rhizome which includes, sesquiterpenes, monoterpenes, nootkatone, 6-methyl-3,5-heptadien-2-one, retinene, nopinone, cycloeucalenol, anozol, toosendanin, furanone, ethanone and vitamin A. Cyperus articulatus Essential Oils (CPEOs) loaded Chitosan Nanoparticles (CSNPs) was successful synthesized using an oil-in-water mixture and ionic gelation method and were characterized by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and UV-Vis spectrophotometry.
30465833	1	78	theme	chemical	282:289	arg1	analysis					303:310	the qualitative chemical composition analysis	266:310	the qualitative chemical composition analysis of the Essential Oils (EOs) by Gas Chromatography-Mass Spectrometry (GC-MS)	266:386	Essential oil of Cyperus articulatus have known bioactivities, the qualitative chemical composition analysis of the Essential Oils (EOs) by Gas Chromatography-Mass Spectrometry (GC-MS) revealed the presence of new compounds not previously known to the plant rhizome which includes, sesquiterpenes, monoterpenes, nootkatone, 6-methyl-3,5-heptadien-2-one, retinene, nopinone, cycloeucalenol, anozol, toosendanin, furanone, ethanone and vitamin A. Cyperus articulatus Essential Oils (CPEOs) loaded Chitosan Nanoparticles (CSNPs) was successful synthesized using an oil-in-water mixture and ionic gelation method and were characterized by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and UV-Vis spectrophotometry.
31837860	8	0	from	groups	1484:1489	arg1	rich					1446:1449	rich	1446:1449	rich	1446:1449	The protein-like components in DOM had the most profound impact on the microbial community, followed by polysaccharides and components rich in hydroxyl and amino functional groups.
31837860	5	1	from	abundance	845:853	arg1	section					871:877	the upstream section	858:877	the upstream section of Xiaohe River	858:893	The bacterial abundance in the upstream section of Xiaohe River was low, while its community structure was unstable but exhibited good uniformity, and the bacterial diversity in the downstream was rich.
31837860	4	2	theme	acid	723:726	arg1	groups					728:733	carboxylic acid groups	712:733	carboxylic acid groups	712:733	The results showed that macromolecular humic acids were the main components of DOM in this river with more carboxylic acid groups and humic-like acid substances found upstream and protein-like substances dominated downstream.
31837860	8	3	theme	functional	1473:1482	arg1	groups					1484:1489	hydroxyl and amino functional groups	1454:1489	groups	1484:1489	The protein-like components in DOM had the most profound impact on the microbial community, followed by polysaccharides and components rich in hydroxyl and amino functional groups.
31837860	0	4	theme	community	88:96	arg1	composition					98:108	dissolved organic matter quality and microbial community composition	41:108	composition	98:108	Municipal wastewater effluent influences dissolved organic matter quality and microbial community composition in an urbanized stream.
31837860	2	5	theme	microbial	362:370	arg1	community					372:380	microbial community	362:380	microbial community	362:380	However, the correlation between effluent-derived DOM and microbial community diversity in urbanized rivers is still poorly understood.
31837860	5	6	from	diversity	996:1004	arg1	downstream					1013:1022	downstream	1013:1022	downstream	1013:1022	The bacterial abundance in the upstream section of Xiaohe River was low, while its community structure was unstable but exhibited good uniformity, and the bacterial diversity in the downstream was rich.
31837860	1	7	theme	aqueous	252:258	arg1	ecosystems					260:269	aqueous ecosystems	252:269	aqueous ecosystems	252:269	Dissolved organic matter (DOM) from wastewater treatment plant (WWTP) effluent poses serious threats to the receiving aqueous ecosystems and their microbial communities.
31837860	9	8	from	effects	1618:1624	arg1	community					1639:1647	microbial community	1629:1647	microbial community in urbanized rivers	1629:1667	The study grasped the migration and evolution of DOM in rivers with unconventional water recharge, and revealed their diverse effects on microbial community in urbanized rivers.
31837860	9	9	dep	migration	1514:1522	arg1	the					1510:1512	the	1510:1512	the	1510:1512	The study grasped the migration and evolution of DOM in rivers with unconventional water recharge, and revealed their diverse effects on microbial community in urbanized rivers.
31837860	9	10	theme	water	1575:1579	arg1	recharge					1581:1588	unconventional water recharge	1560:1588	unconventional water recharge	1560:1588	The study grasped the migration and evolution of DOM in rivers with unconventional water recharge, and revealed their diverse effects on microbial community in urbanized rivers.
31837860	5	11	theme	bacterial	986:994	arg1	rich					1028:1031	rich	1028:1031	rich	1028:1031	The bacterial abundance in the upstream section of Xiaohe River was low, while its community structure was unstable but exhibited good uniformity, and the bacterial diversity in the downstream was rich.
31837860	5	11	theme	bacterial	986:994	arg1	diversity					996:1004	the bacterial diversity	982:1004	the bacterial diversity in the downstream	982:1022	The bacterial abundance in the upstream section of Xiaohe River was low, while its community structure was unstable but exhibited good uniformity, and the bacterial diversity in the downstream was rich.
31837860	9	12	with	rivers	1548:1553	arg1	recharge					1581:1588	unconventional water recharge	1560:1588	unconventional water recharge	1560:1588	The study grasped the migration and evolution of DOM in rivers with unconventional water recharge, and revealed their diverse effects on microbial community in urbanized rivers.
31837860	7	13	theme	different	1242:1250	arg1	compositions					1252:1263	different compositions	1242:1263	different compositions	1242:1263	Furthermore, different microbial communities were affected by different compositions and structure of DOM in the effluent of WWTP.
31837860	9	14	theme	DOM	1541:1543	arg1	evolution					1528:1536	evolution	1528:1536	evolution	1528:1536	The study grasped the migration and evolution of DOM in rivers with unconventional water recharge, and revealed their diverse effects on microbial community in urbanized rivers.
31837860	9	14	theme	DOM	1541:1543	arg1	migration					1514:1522	migration	1514:1522	migration	1514:1522	The study grasped the migration and evolution of DOM in rivers with unconventional water recharge, and revealed their diverse effects on microbial community in urbanized rivers.
31837860	4	15	theme	protein-like	785:796	arg1	substances					798:807	protein-like substances	785:807	protein-like substances dominated downstream	785:828	The results showed that macromolecular humic acids were the main components of DOM in this river with more carboxylic acid groups and humic-like acid substances found upstream and protein-like substances dominated downstream.
31837860	8	16	from	components	1328:1337	arg1	DOM					1342:1344	DOM	1342:1344	DOM	1342:1344	The protein-like components in DOM had the most profound impact on the microbial community, followed by polysaccharides and components rich in hydroxyl and amino functional groups.
31837860	4	17	theme	acid	750:753	arg1	substances					755:764	humic-like acid substances	739:764	humic-like acid substances found upstream	739:779	The results showed that macromolecular humic acids were the main components of DOM in this river with more carboxylic acid groups and humic-like acid substances found upstream and protein-like substances dominated downstream.
31837860	8	18	from	rich	1446:1449	arg1	groups					1484:1489	hydroxyl and amino functional groups	1454:1489	groups	1484:1489	The protein-like components in DOM had the most profound impact on the microbial community, followed by polysaccharides and components rich in hydroxyl and amino functional groups.
31837860	4	19	theme	humic-like	739:748	arg1	substances					755:764	humic-like acid substances	739:764	humic-like acid substances found upstream	739:779	The results showed that macromolecular humic acids were the main components of DOM in this river with more carboxylic acid groups and humic-like acid substances found upstream and protein-like substances dominated downstream.
31837860	4	20	theme	carboxylic	712:721	arg1	groups					728:733	carboxylic acid groups	712:733	carboxylic acid groups	712:733	The results showed that macromolecular humic acids were the main components of DOM in this river with more carboxylic acid groups and humic-like acid substances found upstream and protein-like substances dominated downstream.
31837860	2	21	from	DOM	354:356	arg1	rivers					405:410	urbanized rivers	395:410	urbanized rivers	395:410	However, the correlation between effluent-derived DOM and microbial community diversity in urbanized rivers is still poorly understood.
31837860	8	22	theme	microbial	1382:1390	arg1	community					1392:1400	the microbial community	1378:1400	the microbial community	1378:1400	The protein-like components in DOM had the most profound impact on the microbial community, followed by polysaccharides and components rich in hydroxyl and amino functional groups.
31837860	5	23	theme	good	961:964	arg1	uniformity					966:975	good uniformity	961:975	good uniformity	961:975	The bacterial abundance in the upstream section of Xiaohe River was low, while its community structure was unstable but exhibited good uniformity, and the bacterial diversity in the downstream was rich.
31837860	1	24	theme	wastewater	170:179	arg1	effluent					204:211	wastewater treatment plant (WWTP) effluent	170:211	wastewater treatment plant (WWTP) effluent	170:211	Dissolved organic matter (DOM) from wastewater treatment plant (WWTP) effluent poses serious threats to the receiving aqueous ecosystems and their microbial communities.
31837860	0	25	theme	urbanized	116:124	arg1	stream					126:131	an urbanized stream	113:131	an urbanized stream	113:131	Municipal wastewater effluent influences dissolved organic matter quality and microbial community composition in an urbanized stream.
31837860	6	26	theme	WWTP	1146:1149	arg1	effluent					1151:1158	WWTP effluent	1146:1158	WWTP effluent	1146:1158	The response of bacterial and eukaryotic communities to WWTP effluent was weak, while that of Actinobacteria to WWTP effluent was more prominent.
31837860	2	27	from	community	372:380	arg1	rivers					405:410	urbanized rivers	395:410	urbanized rivers	395:410	However, the correlation between effluent-derived DOM and microbial community diversity in urbanized rivers is still poorly understood.
31837860	4	28	theme	macromolecular	629:642	arg1	components					670:679	the main components	661:679	the main components of DOM	661:686	The results showed that macromolecular humic acids were the main components of DOM in this river with more carboxylic acid groups and humic-like acid substances found upstream and protein-like substances dominated downstream.
31837860	4	28	theme	macromolecular	629:642	arg1	acids					650:654	macromolecular humic acids	629:654	macromolecular humic acids	629:654	The results showed that macromolecular humic acids were the main components of DOM in this river with more carboxylic acid groups and humic-like acid substances found upstream and protein-like substances dominated downstream.
31837860	1	29	theme	treatment	181:189	arg1	effluent					204:211	wastewater treatment plant (WWTP) effluent	170:211	wastewater treatment plant (WWTP) effluent	170:211	Dissolved organic matter (DOM) from wastewater treatment plant (WWTP) effluent poses serious threats to the receiving aqueous ecosystems and their microbial communities.
31837860	0	30	theme	wastewater	10:19	arg1	effluent					21:28	Municipal wastewater effluent	0:28	Municipal wastewater effluent	0:28	Municipal wastewater effluent influences dissolved organic matter quality and microbial community composition in an urbanized stream.
31837860	7	31	theme	WWTP	1305:1308	arg1	effluent					1293:1300	the effluent	1289:1300	the effluent of WWTP	1289:1308	Furthermore, different microbial communities were affected by different compositions and structure of DOM in the effluent of WWTP.
31837860	1	32	theme	plant	191:195	arg1	effluent					204:211	wastewater treatment plant (WWTP) effluent	170:211	wastewater treatment plant (WWTP) effluent	170:211	Dissolved organic matter (DOM) from wastewater treatment plant (WWTP) effluent poses serious threats to the receiving aqueous ecosystems and their microbial communities.
31837860	0	33	theme	Municipal	0:8	arg1	effluent					21:28	Municipal wastewater effluent	0:28	Municipal wastewater effluent	0:28	Municipal wastewater effluent influences dissolved organic matter quality and microbial community composition in an urbanized stream.
31837860	3	34	from	dynamics	513:520	arg1	River					585:589	River	585:589	River	585:589	In this study, the response relationship between the microbial community dynamics and the DOM evolution process in the effluent-dominated Xiaohe River was revealed.
31837860	9	35	from	community	1639:1647	arg1	rivers					1662:1667	urbanized rivers	1652:1667	urbanized rivers	1652:1667	The study grasped the migration and evolution of DOM in rivers with unconventional water recharge, and revealed their diverse effects on microbial community in urbanized rivers.
31837860	2	36	theme	urbanized	395:403	arg1	rivers					405:410	urbanized rivers	395:410	urbanized rivers	395:410	However, the correlation between effluent-derived DOM and microbial community diversity in urbanized rivers is still poorly understood.
31837860	5	37	theme	upstream	862:869	arg1	section					871:877	the upstream section	858:877	the upstream section of Xiaohe River	858:893	The bacterial abundance in the upstream section of Xiaohe River was low, while its community structure was unstable but exhibited good uniformity, and the bacterial diversity in the downstream was rich.
31837860	1	38	theme	microbial	281:289	arg1	communities					291:301	their microbial communities	275:301	their microbial communities	275:301	Dissolved organic matter (DOM) from wastewater treatment plant (WWTP) effluent poses serious threats to the receiving aqueous ecosystems and their microbial communities.
31837860	3	39	from	process	544:550	arg1	River					585:589	River	585:589	River	585:589	In this study, the response relationship between the microbial community dynamics and the DOM evolution process in the effluent-dominated Xiaohe River was revealed.
31837860	6	40	theme	communities	1075:1085	arg1	weak					1108:1111	weak	1108:1111	weak	1108:1111	The response of bacterial and eukaryotic communities to WWTP effluent was weak, while that of Actinobacteria to WWTP effluent was more prominent.
31837860	6	40	theme	communities	1075:1085	arg1	response					1038:1045	The response	1034:1045	The response of bacterial and eukaryotic communities to WWTP effluent	1034:1102	The response of bacterial and eukaryotic communities to WWTP effluent was weak, while that of Actinobacteria to WWTP effluent was more prominent.
31837860	4	41	from	components	670:679	arg1	river					696:700	this river	691:700	this river	691:700	The results showed that macromolecular humic acids were the main components of DOM in this river with more carboxylic acid groups and humic-like acid substances found upstream and protein-like substances dominated downstream.
31837860	1	42	theme	WWTP	198:201	arg1	effluent					204:211	wastewater treatment plant (WWTP) effluent	170:211	wastewater treatment plant (WWTP) effluent	170:211	Dissolved organic matter (DOM) from wastewater treatment plant (WWTP) effluent poses serious threats to the receiving aqueous ecosystems and their microbial communities.
31837860	7	43	theme	different	1193:1201	arg1	communities					1213:1223	different microbial communities	1193:1223	different microbial communities	1193:1223	Furthermore, different microbial communities were affected by different compositions and structure of DOM in the effluent of WWTP.
31837860	7	44	theme	microbial	1203:1211	arg1	communities					1213:1223	different microbial communities	1193:1223	different microbial communities	1193:1223	Furthermore, different microbial communities were affected by different compositions and structure of DOM in the effluent of WWTP.
31837860	2	45	dep	DOM	354:356	arg1	diversity					382:390	diversity	382:390	diversity	382:390	However, the correlation between effluent-derived DOM and microbial community diversity in urbanized rivers is still poorly understood.
31837860	9	46	theme	unconventional	1560:1573	arg1	recharge					1581:1588	unconventional water recharge	1560:1588	unconventional water recharge	1560:1588	The study grasped the migration and evolution of DOM in rivers with unconventional water recharge, and revealed their diverse effects on microbial community in urbanized rivers.
31837860	5	47	theme	community	914:922	arg1	structure					924:932	its community structure	910:932	its community structure	910:932	The bacterial abundance in the upstream section of Xiaohe River was low, while its community structure was unstable but exhibited good uniformity, and the bacterial diversity in the downstream was rich.
31837860	0	48	theme	organic	51:57	arg1	quality					66:72	dissolved organic matter quality and microbial community composition	41:108	quality	66:72	Municipal wastewater effluent influences dissolved organic matter quality and microbial community composition in an urbanized stream.
31837860	8	49	contain	had	1346:1348	arg2	impact					1368:1373	the most profound impact	1350:1373	the most profound impact	1350:1373	The protein-like components in DOM had the most profound impact on the microbial community, followed by polysaccharides and components rich in hydroxyl and amino functional groups.
31837860	8	49	contain	had	1346:1348	arg1	components					1328:1337	The protein-like components	1311:1337	The protein-like components in DOM	1311:1344	The protein-like components in DOM had the most profound impact on the microbial community, followed by polysaccharides and components rich in hydroxyl and amino functional groups.
31837860	5	50	theme	River	889:893	arg1	section					871:877	the upstream section	858:877	the upstream section of Xiaohe River	858:893	The bacterial abundance in the upstream section of Xiaohe River was low, while its community structure was unstable but exhibited good uniformity, and the bacterial diversity in the downstream was rich.
31837860	3	51	theme	response	459:466	arg1	relationship					468:479	the response relationship	455:479	the response relationship between the microbial community dynamics and the DOM evolution process in the effluent-dominated Xiaohe River	455:589	In this study, the response relationship between the microbial community dynamics and the DOM evolution process in the effluent-dominated Xiaohe River was revealed.
31837860	0	52	theme	dissolved	41:49	arg1	quality					66:72	dissolved organic matter quality and microbial community composition	41:108	quality	66:72	Municipal wastewater effluent influences dissolved organic matter quality and microbial community composition in an urbanized stream.
31837860	9	53	theme	microbial	1629:1637	arg1	community					1639:1647	microbial community	1629:1647	microbial community in urbanized rivers	1629:1667	The study grasped the migration and evolution of DOM in rivers with unconventional water recharge, and revealed their diverse effects on microbial community in urbanized rivers.
31837860	1	54	from	effluent	204:211	arg1	DOM					160:162	DOM	160:162	DOM	160:162	Dissolved organic matter (DOM) from wastewater treatment plant (WWTP) effluent poses serious threats to the receiving aqueous ecosystems and their microbial communities.
31837860	1	54	from	effluent	204:211	arg1	matter					152:157	organic matter	144:157	organic matter (DOM) from wastewater treatment plant (WWTP) effluent	144:211	Dissolved organic matter (DOM) from wastewater treatment plant (WWTP) effluent poses serious threats to the receiving aqueous ecosystems and their microbial communities.
31837860	6	55	theme	eukaryotic	1064:1073	arg1	communities					1075:1085	bacterial and eukaryotic communities	1050:1085	bacterial and eukaryotic communities	1050:1085	The response of bacterial and eukaryotic communities to WWTP effluent was weak, while that of Actinobacteria to WWTP effluent was more prominent.
31837860	5	56	theme	bacterial	835:843	arg1	abundance					845:853	The bacterial abundance	831:853	The bacterial abundance in the upstream section of Xiaohe River	831:893	The bacterial abundance in the upstream section of Xiaohe River was low, while its community structure was unstable but exhibited good uniformity, and the bacterial diversity in the downstream was rich.
31837860	5	56	theme	bacterial	835:843	arg1	low					899:901	low	899:901	low	899:901	The bacterial abundance in the upstream section of Xiaohe River was low, while its community structure was unstable but exhibited good uniformity, and the bacterial diversity in the downstream was rich.
31837860	8	57	theme	rich	1446:1449	arg1	polysaccharides					1415:1429	polysaccharides	1415:1429	polysaccharides	1415:1429	The protein-like components in DOM had the most profound impact on the microbial community, followed by polysaccharides and components rich in hydroxyl and amino functional groups.
31837860	0	58	theme	matter	59:64	arg1	quality					66:72	dissolved organic matter quality and microbial community composition	41:108	quality	66:72	Municipal wastewater effluent influences dissolved organic matter quality and microbial community composition in an urbanized stream.
31837860	3	59	theme	microbial	493:501	arg1	dynamics					513:520	the microbial community dynamics	489:520	the microbial community dynamics	489:520	In this study, the response relationship between the microbial community dynamics and the DOM evolution process in the effluent-dominated Xiaohe River was revealed.
31837860	3	60	theme	DOM	530:532	arg1	process					544:550	the DOM evolution process	526:550	the DOM evolution process	526:550	In this study, the response relationship between the microbial community dynamics and the DOM evolution process in the effluent-dominated Xiaohe River was revealed.
31837860	8	61	theme	protein-like	1315:1326	arg1	components					1328:1337	The protein-like components	1311:1337	The protein-like components in DOM	1311:1344	The protein-like components in DOM had the most profound impact on the microbial community, followed by polysaccharides and components rich in hydroxyl and amino functional groups.
31837860	9	62	theme	urbanized	1652:1660	arg1	rivers					1662:1667	urbanized rivers	1652:1667	urbanized rivers	1652:1667	The study grasped the migration and evolution of DOM in rivers with unconventional water recharge, and revealed their diverse effects on microbial community in urbanized rivers.
31837860	4	63	with	components	670:679	arg1	substances					798:807	protein-like substances	785:807	protein-like substances dominated downstream	785:828	The results showed that macromolecular humic acids were the main components of DOM in this river with more carboxylic acid groups and humic-like acid substances found upstream and protein-like substances dominated downstream.
31837860	4	63	with	components	670:679	arg1	substances					755:764	humic-like acid substances	739:764	humic-like acid substances found upstream	739:779	The results showed that macromolecular humic acids were the main components of DOM in this river with more carboxylic acid groups and humic-like acid substances found upstream and protein-like substances dominated downstream.
31837860	4	63	with	components	670:679	arg1	groups					728:733	carboxylic acid groups	712:733	carboxylic acid groups	712:733	The results showed that macromolecular humic acids were the main components of DOM in this river with more carboxylic acid groups and humic-like acid substances found upstream and protein-like substances dominated downstream.
31837860	8	64	theme	hydroxyl	1454:1461	arg1	groups					1484:1489	hydroxyl and amino functional groups	1454:1489	groups	1484:1489	The protein-like components in DOM had the most profound impact on the microbial community, followed by polysaccharides and components rich in hydroxyl and amino functional groups.
31837860	6	65	theme	bacterial	1050:1058	arg1	communities					1075:1085	bacterial and eukaryotic communities	1050:1085	bacterial and eukaryotic communities	1050:1085	The response of bacterial and eukaryotic communities to WWTP effluent was weak, while that of Actinobacteria to WWTP effluent was more prominent.
31837860	1	66	theme	organic	144:150	arg1	DOM					160:162	DOM	160:162	DOM	160:162	Dissolved organic matter (DOM) from wastewater treatment plant (WWTP) effluent poses serious threats to the receiving aqueous ecosystems and their microbial communities.
31837860	1	66	theme	organic	144:150	arg1	matter					152:157	organic matter	144:157	organic matter (DOM) from wastewater treatment plant (WWTP) effluent	144:211	Dissolved organic matter (DOM) from wastewater treatment plant (WWTP) effluent poses serious threats to the receiving aqueous ecosystems and their microbial communities.
31837860	9	67	theme	diverse	1610:1616	arg1	effects					1618:1624	their diverse effects	1604:1624	their diverse effects on microbial community in urbanized rivers	1604:1667	The study grasped the migration and evolution of DOM in rivers with unconventional water recharge, and revealed their diverse effects on microbial community in urbanized rivers.
31837860	1	68	theme	serious	219:225	arg1	threats					227:233	serious threats	219:233	serious threats to the receiving aqueous ecosystems and their microbial communities	219:301	Dissolved organic matter (DOM) from wastewater treatment plant (WWTP) effluent poses serious threats to the receiving aqueous ecosystems and their microbial communities.
31837860	6	69	theme	WWTP	1090:1093	arg1	effluent					1095:1102	WWTP effluent	1090:1102	WWTP effluent	1090:1102	The response of bacterial and eukaryotic communities to WWTP effluent was weak, while that of Actinobacteria to WWTP effluent was more prominent.
31837860	3	70	theme	community	503:511	arg1	dynamics					513:520	the microbial community dynamics	489:520	the microbial community dynamics	489:520	In this study, the response relationship between the microbial community dynamics and the DOM evolution process in the effluent-dominated Xiaohe River was revealed.
31837860	8	71	theme	profound	1359:1366	arg1	impact					1368:1373	the most profound impact	1350:1373	the most profound impact	1350:1373	The protein-like components in DOM had the most profound impact on the microbial community, followed by polysaccharides and components rich in hydroxyl and amino functional groups.
31837860	2	72	theme	effluent-derived	337:352	arg1	DOM					354:356	effluent-derived DOM	337:356	effluent-derived DOM	337:356	However, the correlation between effluent-derived DOM and microbial community diversity in urbanized rivers is still poorly understood.
31837860	0	73	theme	microbial	78:86	arg1	composition					98:108	dissolved organic matter quality and microbial community composition	41:108	composition	98:108	Municipal wastewater effluent influences dissolved organic matter quality and microbial community composition in an urbanized stream.
31837860	8	74	theme	amino	1467:1471	arg1	groups					1484:1489	hydroxyl and amino functional groups	1454:1489	groups	1484:1489	The protein-like components in DOM had the most profound impact on the microbial community, followed by polysaccharides and components rich in hydroxyl and amino functional groups.
31837860	4	75	theme	main	665:668	arg1	components					670:679	the main components	661:679	the main components of DOM	661:686	The results showed that macromolecular humic acids were the main components of DOM in this river with more carboxylic acid groups and humic-like acid substances found upstream and protein-like substances dominated downstream.
31837860	4	75	theme	main	665:668	arg1	acids					650:654	macromolecular humic acids	629:654	macromolecular humic acids	629:654	The results showed that macromolecular humic acids were the main components of DOM in this river with more carboxylic acid groups and humic-like acid substances found upstream and protein-like substances dominated downstream.
31837860	3	76	theme	evolution	534:542	arg1	process					544:550	the DOM evolution process	526:550	the DOM evolution process	526:550	In this study, the response relationship between the microbial community dynamics and the DOM evolution process in the effluent-dominated Xiaohe River was revealed.
31837860	4	77	theme	DOM	684:686	arg1	components					670:679	the main components	661:679	the main components of DOM	661:686	The results showed that macromolecular humic acids were the main components of DOM in this river with more carboxylic acid groups and humic-like acid substances found upstream and protein-like substances dominated downstream.
31837860	4	77	theme	DOM	684:686	arg1	acids					650:654	macromolecular humic acids	629:654	macromolecular humic acids	629:654	The results showed that macromolecular humic acids were the main components of DOM in this river with more carboxylic acid groups and humic-like acid substances found upstream and protein-like substances dominated downstream.
31837860	2	78	link	effluent-derived	337:352	arg1	DOM					354:356	effluent-derived DOM	337:356	effluent-derived DOM	337:356	However, the correlation between effluent-derived DOM and microbial community diversity in urbanized rivers is still poorly understood.
31837860	4	79	theme	humic	644:648	arg1	components					670:679	the main components	661:679	the main components of DOM	661:686	The results showed that macromolecular humic acids were the main components of DOM in this river with more carboxylic acid groups and humic-like acid substances found upstream and protein-like substances dominated downstream.
31837860	4	79	theme	humic	644:648	arg1	acids					650:654	macromolecular humic acids	629:654	macromolecular humic acids	629:654	The results showed that macromolecular humic acids were the main components of DOM in this river with more carboxylic acid groups and humic-like acid substances found upstream and protein-like substances dominated downstream.
31837860	7	80	theme	DOM	1282:1284	arg1	structure					1269:1277	structure	1269:1277	structure	1269:1277	Furthermore, different microbial communities were affected by different compositions and structure of DOM in the effluent of WWTP.
31837860	7	80	theme	DOM	1282:1284	arg1	compositions					1252:1263	different compositions	1242:1263	different compositions	1242:1263	Furthermore, different microbial communities were affected by different compositions and structure of DOM in the effluent of WWTP.
31308664	10	0	theme	morphogenetic	1599:1611	arg1	collagen-1					1582:1591	collagen-1	1582:1591	collagen-1	1582:1591	The in vitro studies demonstrated that the SV/CS/PET-HA ligaments induced osteogenic differentiation in the MC3T3 cells, with higher mRNA expression levels of collagen-1, bone morphogenetic protein-2, osteocalcin and alkaline phosphatase than those in the CS/PET-HA and PET-HA ligament groups.
31308664	10	0	theme	morphogenetic	1599:1611	arg1	protein-2					1613:1621	bone morphogenetic protein-2	1594:1621	bone morphogenetic protein-2	1594:1621	The in vitro studies demonstrated that the SV/CS/PET-HA ligaments induced osteogenic differentiation in the MC3T3 cells, with higher mRNA expression levels of collagen-1, bone morphogenetic protein-2, osteocalcin and alkaline phosphatase than those in the CS/PET-HA and PET-HA ligament groups.
31308664	1	1	theme	-coated	296:302	arg1	ligaments					337:345	plasma-sprayed hydroxyapatite (HA)-coated polyethylene terephthalate (PET) ligaments	262:345	plasma-sprayed hydroxyapatite (HA)-coated polyethylene terephthalate (PET) ligaments encapsulated with a simvastatin (SV)-chitosan (CS) composite	262:406	PURPOSE This study was designed to evaluate the in vitro and in vivo biocompatibility and osteointegration of plasma-sprayed hydroxyapatite (HA)-coated polyethylene terephthalate (PET) ligaments encapsulated with a simvastatin (SV)-chitosan (CS) composite.
31308664	3	2	theme	emission	555:562	arg1	microscope					582:591	A field emission scanning electron microscope	547:591	A field emission scanning electron microscope	547:591	A field emission scanning electron microscope was used to characterize the morphology, and the in vitro SV release profile was analyzed.
31308664	1	3	theme	terephthalate	317:329	arg1	ligaments					337:345	plasma-sprayed hydroxyapatite (HA)-coated polyethylene terephthalate (PET) ligaments	262:345	plasma-sprayed hydroxyapatite (HA)-coated polyethylene terephthalate (PET) ligaments encapsulated with a simvastatin (SV)-chitosan (CS) composite	262:406	PURPOSE This study was designed to evaluate the in vitro and in vivo biocompatibility and osteointegration of plasma-sprayed hydroxyapatite (HA)-coated polyethylene terephthalate (PET) ligaments encapsulated with a simvastatin (SV)-chitosan (CS) composite.
31308664	12	4	theme	HA-coated	2234:2242	arg1	ligaments					2248:2256	plasma-sprayed HA-coated PET ligaments	2219:2256	plasma-sprayed HA-coated PET ligaments	2219:2256	CONCLUSION The SV-CS composite nanoporous structure was associated with the improved biocompatibility and osteogenic differentiation in vitro and enhanced osteointegration process in vivo of plasma-sprayed HA-coated PET ligaments.
31308664	10	5	dep	in	1427:1428	arg1	vitro					1430:1434	vitro	1430:1434	vitro	1430:1434	The in vitro studies demonstrated that the SV/CS/PET-HA ligaments induced osteogenic differentiation in the MC3T3 cells, with higher mRNA expression levels of collagen-1, bone morphogenetic protein-2, osteocalcin and alkaline phosphatase than those in the CS/PET-HA and PET-HA ligament groups.
31308664	8	6	theme	plasma-sprayed	1238:1251	arg1	particles					1256:1264	the plasma-sprayed HA particles	1234:1264	the plasma-sprayed HA particles	1234:1264	RESULTS The characteristic nanoporous structures mainly formed on the surface of the plasma-sprayed HA particles in the SV/CS/PET-HA and CS/PET-HA groups.
31308664	12	7	theme	ligaments	2248:2256	arg1	differentiation					2145:2159	osteogenic differentiation	2134:2159	osteogenic differentiation	2134:2159	CONCLUSION The SV-CS composite nanoporous structure was associated with the improved biocompatibility and osteogenic differentiation in vitro and enhanced osteointegration process in vivo of plasma-sprayed HA-coated PET ligaments.
31308664	12	7	theme	ligaments	2248:2256	arg1	process					2200:2206	enhanced osteointegration process	2174:2206	enhanced osteointegration process in vivo of plasma-sprayed HA-coated PET ligaments	2174:2256	CONCLUSION The SV-CS composite nanoporous structure was associated with the improved biocompatibility and osteogenic differentiation in vitro and enhanced osteointegration process in vivo of plasma-sprayed HA-coated PET ligaments.
31308664	12	7	theme	ligaments	2248:2256	arg1	biocompatibility					2113:2128	improved biocompatibility	2104:2128	improved biocompatibility	2104:2128	CONCLUSION The SV-CS composite nanoporous structure was associated with the improved biocompatibility and osteogenic differentiation in vitro and enhanced osteointegration process in vivo of plasma-sprayed HA-coated PET ligaments.
31308664	11	8	theme	implant	1858:1864	arg1	contact					1866:1872	bone implant contact and interface area	1853:1891	contact	1866:1872	The in vivo tests showed that both micro-CT analysis (bone mineral density and bone volume per total volume) and histological analysis (bone implant contact and interface area) revealed significantly higher peri-implant bone formation and less interface area in the SV/CS/PET-HA group than in the other groups.
31308664	12	9	dep	CONCLUSION	2028:2037	arg1	associated					2084:2093	associated	2084:2093	was associated with the improved biocompatibility and osteogenic differentiation in vitro and enhanced osteointegration process in vivo of plasma-sprayed HA-coated PET ligaments	2080:2256	CONCLUSION The SV-CS composite nanoporous structure was associated with the improved biocompatibility and osteogenic differentiation in vitro and enhanced osteointegration process in vivo of plasma-sprayed HA-coated PET ligaments.
31308664	10	10	theme	SV/CS/PET-HA	1466:1477	arg1	ligaments					1479:1487	the SV/CS/PET-HA ligaments	1462:1487	the SV/CS/PET-HA ligaments	1462:1487	The in vitro studies demonstrated that the SV/CS/PET-HA ligaments induced osteogenic differentiation in the MC3T3 cells, with higher mRNA expression levels of collagen-1, bone morphogenetic protein-2, osteocalcin and alkaline phosphatase than those in the CS/PET-HA and PET-HA ligament groups.
31308664	8	11	theme	particles	1256:1264	arg1	surface					1223:1229	the surface	1219:1229	the surface of the plasma-sprayed HA particles	1219:1264	RESULTS The characteristic nanoporous structures mainly formed on the surface of the plasma-sprayed HA particles in the SV/CS/PET-HA and CS/PET-HA groups.
31308664	6	12	theme	male	995:998	arg1	rats					1015:1018	male Sprague-Dawley rats	995:1018	male Sprague-Dawley rats	995:1018	Osteointegration was performed by implanting the PET ligaments into the proximal tibia bone tunnels of male Sprague-Dawley rats for 3 weeks and 6 weeks.
31308664	3	13	theme	electron	573:580	arg1	microscope					582:591	A field emission scanning electron microscope	547:591	A field emission scanning electron microscope	547:591	A field emission scanning electron microscope was used to characterize the morphology, and the in vitro SV release profile was analyzed.
31308664	10	14	theme	ligament	1700:1707	arg1	groups					1709:1714	the CS/PET-HA and PET-HA ligament groups	1675:1714	groups	1709:1714	The in vitro studies demonstrated that the SV/CS/PET-HA ligaments induced osteogenic differentiation in the MC3T3 cells, with higher mRNA expression levels of collagen-1, bone morphogenetic protein-2, osteocalcin and alkaline phosphatase than those in the CS/PET-HA and PET-HA ligament groups.
31308664	5	15	theme	genes	874:878	arg1	expression					853:862	the expression	849:862	the expression of marker genes using qPCR	849:889	Osteogenic differentiation was investigated by the expression of marker genes using qPCR.
31308664	6	16	theme	tibia	973:977	arg1	tunnels					984:990	the proximal tibia bone tunnels	960:990	the proximal tibia bone tunnels of male Sprague-Dawley rats for 3 weeks and 6 weeks	960:1042	Osteointegration was performed by implanting the PET ligaments into the proximal tibia bone tunnels of male Sprague-Dawley rats for 3 weeks and 6 weeks.
31308664	9	17	theme	SV	1312:1313	arg1	test					1323:1326	The SV release test	1308:1326	The SV release test	1308:1326	The SV release test showed that the sustained release of simvastatin lasted for 25 days in the SV/CS/PET-HA group.
31308664	11	18	theme	other	2014:2018	arg1	groups					2020:2025	the other groups	2010:2025	the other groups	2010:2025	The in vivo tests showed that both micro-CT analysis (bone mineral density and bone volume per total volume) and histological analysis (bone implant contact and interface area) revealed significantly higher peri-implant bone formation and less interface area in the SV/CS/PET-HA group than in the other groups.
31308664	12	19	theme	osteointegration	2183:2198	arg1	process					2200:2206	enhanced osteointegration process	2174:2206	enhanced osteointegration process in vivo of plasma-sprayed HA-coated PET ligaments	2174:2256	CONCLUSION The SV-CS composite nanoporous structure was associated with the improved biocompatibility and osteogenic differentiation in vitro and enhanced osteointegration process in vivo of plasma-sprayed HA-coated PET ligaments.
31308664	11	20	theme	total	1812:1816	arg1	volume					1818:1823	total volume	1812:1823	total volume	1812:1823	The in vivo tests showed that both micro-CT analysis (bone mineral density and bone volume per total volume) and histological analysis (bone implant contact and interface area) revealed significantly higher peri-implant bone formation and less interface area in the SV/CS/PET-HA group than in the other groups.
31308664	12	21	theme	osteogenic	2134:2143	arg1	differentiation					2145:2159	osteogenic differentiation	2134:2159	osteogenic differentiation	2134:2159	CONCLUSION The SV-CS composite nanoporous structure was associated with the improved biocompatibility and osteogenic differentiation in vitro and enhanced osteointegration process in vivo of plasma-sprayed HA-coated PET ligaments.
31308664	11	22	theme	histological	1830:1841	arg1	analysis					1843:1850	histological analysis	1830:1850	histological analysis (bone implant contact and interface area)	1830:1892	The in vivo tests showed that both micro-CT analysis (bone mineral density and bone volume per total volume) and histological analysis (bone implant contact and interface area) revealed significantly higher peri-implant bone formation and less interface area in the SV/CS/PET-HA group than in the other groups.
31308664	0	23	theme	hydroxyapatite-coated	92:112	arg1	ligaments					141:149	hydroxyapatite-coated polyethylene terephthalate ligaments	92:149	hydroxyapatite-coated polyethylene terephthalate ligaments	92:149	Encapsulation of a nanoporous simvastatin-chitosan composite to enhance osteointegration of hydroxyapatite-coated polyethylene terephthalate ligaments.
31308664	9	24	theme	sustained	1344:1352	arg1	release					1354:1360	the sustained release	1340:1360	the sustained release of simvastatin	1340:1375	The SV release test showed that the sustained release of simvastatin lasted for 25 days in the SV/CS/PET-HA group.
31308664	1	25	theme	in	200:201	arg1	biocompatibility					221:236	in vitro and in vivo biocompatibility	200:236	in vitro and in vivo biocompatibility	200:236	PURPOSE This study was designed to evaluate the in vitro and in vivo biocompatibility and osteointegration of plasma-sprayed hydroxyapatite (HA)-coated polyethylene terephthalate (PET) ligaments encapsulated with a simvastatin (SV)-chitosan (CS) composite.
31308664	0	26	theme	terephthalate	127:139	arg1	ligaments					141:149	hydroxyapatite-coated polyethylene terephthalate ligaments	92:149	hydroxyapatite-coated polyethylene terephthalate ligaments	92:149	Encapsulation of a nanoporous simvastatin-chitosan composite to enhance osteointegration of hydroxyapatite-coated polyethylene terephthalate ligaments.
31308664	6	27	theme	rats	1015:1018	arg1	tunnels					984:990	the proximal tibia bone tunnels	960:990	the proximal tibia bone tunnels of male Sprague-Dawley rats for 3 weeks and 6 weeks	960:1042	Osteointegration was performed by implanting the PET ligaments into the proximal tibia bone tunnels of male Sprague-Dawley rats for 3 weeks and 6 weeks.
31308664	4	28	theme	CCK-8	789:793	arg1	tests					795:799	CCK-8 tests	789:799	CCK-8 tests	789:799	MC3T3 cells were cocultured with SV/CS/PET-HA, CS/PET-HA and PET-HA to test their biocompatibility using CCK-8 tests.
31308664	2	29	theme	bone	462:465	arg1	responses					467:475	the in vitro and in vivo bone responses	437:475	the in vitro and in vivo bone responses to three different PET ligaments	437:508	METHODS This study compared the in vitro and in vivo bone responses to three different PET ligaments: SV/CS/PET-HA, CS/PET-HA and PET-HA.
31308664	9	30	theme	SV/CS/PET-HA	1403:1414	arg1	group					1416:1420	the SV/CS/PET-HA group	1399:1420	the SV/CS/PET-HA group	1399:1420	The SV release test showed that the sustained release of simvastatin lasted for 25 days in the SV/CS/PET-HA group.
31308664	11	31	theme	interface	1961:1969	arg1	area					1971:1974	less interface area	1956:1974	less interface area	1956:1974	The in vivo tests showed that both micro-CT analysis (bone mineral density and bone volume per total volume) and histological analysis (bone implant contact and interface area) revealed significantly higher peri-implant bone formation and less interface area in the SV/CS/PET-HA group than in the other groups.
31308664	3	32	theme	release	654:660	arg1	profile					662:668	the in vitro SV release profile	638:668	the in vitro SV release profile	638:668	A field emission scanning electron microscope was used to characterize the morphology, and the in vitro SV release profile was analyzed.
31308664	2	33	theme	in	454:455	arg1	responses					467:475	the in vitro and in vivo bone responses	437:475	the in vitro and in vivo bone responses to three different PET ligaments	437:508	METHODS This study compared the in vitro and in vivo bone responses to three different PET ligaments: SV/CS/PET-HA, CS/PET-HA and PET-HA.
31308664	1	34	dep	-chitosan	383:391	arg1	CS					394:395	CS	394:395	CS	394:395	PURPOSE This study was designed to evaluate the in vitro and in vivo biocompatibility and osteointegration of plasma-sprayed hydroxyapatite (HA)-coated polyethylene terephthalate (PET) ligaments encapsulated with a simvastatin (SV)-chitosan (CS) composite.
31308664	11	35	dep	in	1721:1722	arg1	vivo					1724:1727	vivo	1724:1727	vivo	1724:1727	The in vivo tests showed that both micro-CT analysis (bone mineral density and bone volume per total volume) and histological analysis (bone implant contact and interface area) revealed significantly higher peri-implant bone formation and less interface area in the SV/CS/PET-HA group than in the other groups.
31308664	2	36	theme	different	486:494	arg1	ligaments					500:508	three different PET ligaments	480:508	three different PET ligaments	480:508	METHODS This study compared the in vitro and in vivo bone responses to three different PET ligaments: SV/CS/PET-HA, CS/PET-HA and PET-HA.
31308664	10	37	theme	mRNA	1556:1559	arg1	levels					1572:1577	higher mRNA expression levels	1549:1577	higher mRNA expression levels of collagen-1, bone morphogenetic protein-2, osteocalcin and alkaline phosphatase than those in the CS/PET-HA and PET-HA ligament groups	1549:1714	The in vitro studies demonstrated that the SV/CS/PET-HA ligaments induced osteogenic differentiation in the MC3T3 cells, with higher mRNA expression levels of collagen-1, bone morphogenetic protein-2, osteocalcin and alkaline phosphatase than those in the CS/PET-HA and PET-HA ligament groups.
31308664	11	38	theme	bone	1771:1774	arg1	density					1784:1790	bone mineral density	1771:1790	bone mineral density	1771:1790	The in vivo tests showed that both micro-CT analysis (bone mineral density and bone volume per total volume) and histological analysis (bone implant contact and interface area) revealed significantly higher peri-implant bone formation and less interface area in the SV/CS/PET-HA group than in the other groups.
31308664	1	39	dep	in	213:214	arg1	vivo					216:219	vivo	216:219	vivo	216:219	PURPOSE This study was designed to evaluate the in vitro and in vivo biocompatibility and osteointegration of plasma-sprayed hydroxyapatite (HA)-coated polyethylene terephthalate (PET) ligaments encapsulated with a simvastatin (SV)-chitosan (CS) composite.
31308664	11	40	theme	SV/CS/PET-HA	1983:1994	arg1	group					1996:2000	the SV/CS/PET-HA group	1979:2000	the SV/CS/PET-HA group	1979:2000	The in vivo tests showed that both micro-CT analysis (bone mineral density and bone volume per total volume) and histological analysis (bone implant contact and interface area) revealed significantly higher peri-implant bone formation and less interface area in the SV/CS/PET-HA group than in the other groups.
31308664	0	41	theme	simvastatin-chitosan	30:49	arg1	composite					51:59	a nanoporous simvastatin-chitosan composite	17:59	a nanoporous simvastatin-chitosan composite	17:59	Encapsulation of a nanoporous simvastatin-chitosan composite to enhance osteointegration of hydroxyapatite-coated polyethylene terephthalate ligaments.
31308664	10	42	theme	bone	1594:1597	arg1	collagen-1					1582:1591	collagen-1	1582:1591	collagen-1	1582:1591	The in vitro studies demonstrated that the SV/CS/PET-HA ligaments induced osteogenic differentiation in the MC3T3 cells, with higher mRNA expression levels of collagen-1, bone morphogenetic protein-2, osteocalcin and alkaline phosphatase than those in the CS/PET-HA and PET-HA ligament groups.
31308664	10	42	theme	bone	1594:1597	arg1	protein-2					1613:1621	bone morphogenetic protein-2	1594:1621	bone morphogenetic protein-2	1594:1621	The in vitro studies demonstrated that the SV/CS/PET-HA ligaments induced osteogenic differentiation in the MC3T3 cells, with higher mRNA expression levels of collagen-1, bone morphogenetic protein-2, osteocalcin and alkaline phosphatase than those in the CS/PET-HA and PET-HA ligament groups.
31308664	11	43	theme	higher	1917:1922	arg1	formation					1942:1950	significantly higher peri-implant bone formation	1903:1950	significantly higher peri-implant bone formation	1903:1950	The in vivo tests showed that both micro-CT analysis (bone mineral density and bone volume per total volume) and histological analysis (bone implant contact and interface area) revealed significantly higher peri-implant bone formation and less interface area in the SV/CS/PET-HA group than in the other groups.
31308664	3	44	theme	in	642:643	arg1	profile					662:668	the in vitro SV release profile	638:668	the in vitro SV release profile	638:668	A field emission scanning electron microscope was used to characterize the morphology, and the in vitro SV release profile was analyzed.
31308664	10	45	theme	collagen-1	1582:1591	arg1	levels					1572:1577	higher mRNA expression levels	1549:1577	higher mRNA expression levels of collagen-1, bone morphogenetic protein-2, osteocalcin and alkaline phosphatase than those in the CS/PET-HA and PET-HA ligament groups	1549:1714	The in vitro studies demonstrated that the SV/CS/PET-HA ligaments induced osteogenic differentiation in the MC3T3 cells, with higher mRNA expression levels of collagen-1, bone morphogenetic protein-2, osteocalcin and alkaline phosphatase than those in the CS/PET-HA and PET-HA ligament groups.
31308664	11	46	theme	bone	1796:1799	arg1	volume					1801:1806	bone volume	1796:1806	bone volume	1796:1806	The in vivo tests showed that both micro-CT analysis (bone mineral density and bone volume per total volume) and histological analysis (bone implant contact and interface area) revealed significantly higher peri-implant bone formation and less interface area in the SV/CS/PET-HA group than in the other groups.
31308664	2	47	dep	METHODS	409:415	arg1	CS/PET-HA					525:533	CS/PET-HA	525:533	CS/PET-HA	525:533	METHODS This study compared the in vitro and in vivo bone responses to three different PET ligaments: SV/CS/PET-HA, CS/PET-HA and PET-HA.
31308664	2	47	dep	METHODS	409:415	arg1	SV/CS/PET-HA					511:522	SV/CS/PET-HA	511:522	SV/CS/PET-HA	511:522	METHODS This study compared the in vitro and in vivo bone responses to three different PET ligaments: SV/CS/PET-HA, CS/PET-HA and PET-HA.
31308664	2	47	dep	METHODS	409:415	arg1	METHODS					409:415	METHODS	409:415	METHODS This study compared the in vitro and in vivo bone responses to three different PET ligaments: SV/CS/PET-HA, CS/PET-HA and PET-HA.	409:545	METHODS This study compared the in vitro and in vivo bone responses to three different PET ligaments: SV/CS/PET-HA, CS/PET-HA and PET-HA.
31308664	2	47	dep	METHODS	409:415	arg1	compared					428:435	compared	428:435	compared the in vitro and in vivo bone responses to three different PET ligaments	428:508	METHODS This study compared the in vitro and in vivo bone responses to three different PET ligaments: SV/CS/PET-HA, CS/PET-HA and PET-HA.
31308664	2	47	dep	METHODS	409:415	arg1	PET-HA					539:544	PET-HA	539:544	PET-HA	539:544	METHODS This study compared the in vitro and in vivo bone responses to three different PET ligaments: SV/CS/PET-HA, CS/PET-HA and PET-HA.
31308664	3	48	dep	in	642:643	arg1	vitro					645:649	vitro	645:649	vitro	645:649	A field emission scanning electron microscope was used to characterize the morphology, and the in vitro SV release profile was analyzed.
31308664	4	49	theme	MC3T3	684:688	arg1	cells					690:694	MC3T3 cells	684:694	MC3T3 cells	684:694	MC3T3 cells were cocultured with SV/CS/PET-HA, CS/PET-HA and PET-HA to test their biocompatibility using CCK-8 tests.
31308664	1	50	dep	biocompatibility	221:236	arg1	the					196:198	the	196:198	the	196:198	PURPOSE This study was designed to evaluate the in vitro and in vivo biocompatibility and osteointegration of plasma-sprayed hydroxyapatite (HA)-coated polyethylene terephthalate (PET) ligaments encapsulated with a simvastatin (SV)-chitosan (CS) composite.
31308664	12	51	theme	plasma-sprayed	2219:2232	arg1	ligaments					2248:2256	plasma-sprayed HA-coated PET ligaments	2219:2256	plasma-sprayed HA-coated PET ligaments	2219:2256	CONCLUSION The SV-CS composite nanoporous structure was associated with the improved biocompatibility and osteogenic differentiation in vitro and enhanced osteointegration process in vivo of plasma-sprayed HA-coated PET ligaments.
31308664	8	52	theme	nanoporous	1180:1189	arg1	structures					1191:1200	The characteristic nanoporous structures	1161:1200	The characteristic nanoporous structures mainly formed on the surface of the plasma-sprayed HA particles in the SV/CS/PET-HA and CS/PET-HA groups	1161:1305	RESULTS The characteristic nanoporous structures mainly formed on the surface of the plasma-sprayed HA particles in the SV/CS/PET-HA and CS/PET-HA groups.
31308664	11	53	theme	bone	1937:1940	arg1	formation					1942:1950	significantly higher peri-implant bone formation	1903:1950	significantly higher peri-implant bone formation	1903:1950	The in vivo tests showed that both micro-CT analysis (bone mineral density and bone volume per total volume) and histological analysis (bone implant contact and interface area) revealed significantly higher peri-implant bone formation and less interface area in the SV/CS/PET-HA group than in the other groups.
31308664	12	54	dep	biocompatibility	2113:2128	arg1	the					2100:2102	the	2100:2102	the	2100:2102	CONCLUSION The SV-CS composite nanoporous structure was associated with the improved biocompatibility and osteogenic differentiation in vitro and enhanced osteointegration process in vivo of plasma-sprayed HA-coated PET ligaments.
31308664	12	55	theme	improved	2104:2111	arg1	biocompatibility					2113:2128	improved biocompatibility	2104:2128	improved biocompatibility	2104:2128	CONCLUSION The SV-CS composite nanoporous structure was associated with the improved biocompatibility and osteogenic differentiation in vitro and enhanced osteointegration process in vivo of plasma-sprayed HA-coated PET ligaments.
31308664	6	56	theme	PET	941:943	arg1	ligaments					945:953	the PET ligaments	937:953	the PET ligaments	937:953	Osteointegration was performed by implanting the PET ligaments into the proximal tibia bone tunnels of male Sprague-Dawley rats for 3 weeks and 6 weeks.
31308664	3	57	theme	field	549:553	arg1	microscope					582:591	A field emission scanning electron microscope	547:591	A field emission scanning electron microscope	547:591	A field emission scanning electron microscope was used to characterize the morphology, and the in vitro SV release profile was analyzed.
31308664	1	58	theme	polyethylene	304:315	arg1	ligaments					337:345	plasma-sprayed hydroxyapatite (HA)-coated polyethylene terephthalate (PET) ligaments	262:345	plasma-sprayed hydroxyapatite (HA)-coated polyethylene terephthalate (PET) ligaments encapsulated with a simvastatin (SV)-chitosan (CS) composite	262:406	PURPOSE This study was designed to evaluate the in vitro and in vivo biocompatibility and osteointegration of plasma-sprayed hydroxyapatite (HA)-coated polyethylene terephthalate (PET) ligaments encapsulated with a simvastatin (SV)-chitosan (CS) composite.
31308664	12	59	theme	nanoporous	2059:2068	arg1	structure					2070:2078	The SV-CS composite nanoporous structure	2039:2078	The SV-CS composite nanoporous structure	2039:2078	CONCLUSION The SV-CS composite nanoporous structure was associated with the improved biocompatibility and osteogenic differentiation in vitro and enhanced osteointegration process in vivo of plasma-sprayed HA-coated PET ligaments.
31308664	10	60	theme	alkaline	1640:1647	arg1	collagen-1					1582:1591	collagen-1	1582:1591	collagen-1	1582:1591	The in vitro studies demonstrated that the SV/CS/PET-HA ligaments induced osteogenic differentiation in the MC3T3 cells, with higher mRNA expression levels of collagen-1, bone morphogenetic protein-2, osteocalcin and alkaline phosphatase than those in the CS/PET-HA and PET-HA ligament groups.
31308664	10	60	theme	alkaline	1640:1647	arg1	phosphatase					1649:1659	alkaline phosphatase	1640:1659	alkaline phosphatase	1640:1659	The in vitro studies demonstrated that the SV/CS/PET-HA ligaments induced osteogenic differentiation in the MC3T3 cells, with higher mRNA expression levels of collagen-1, bone morphogenetic protein-2, osteocalcin and alkaline phosphatase than those in the CS/PET-HA and PET-HA ligament groups.
31308664	7	61	theme	histological	1130:1141	arg1	analysis					1143:1150	histological analysis	1130:1150	histological analysis	1130:1150	The bone-implant interface was evaluated by micro-computed tomography (micro-CT) and histological analysis.
31308664	8	62	theme	CS/PET-HA	1290:1298	arg1	groups					1300:1305	the SV/CS/PET-HA and CS/PET-HA groups	1269:1305	the SV/CS/PET-HA and CS/PET-HA groups	1269:1305	RESULTS The characteristic nanoporous structures mainly formed on the surface of the plasma-sprayed HA particles in the SV/CS/PET-HA and CS/PET-HA groups.
31308664	1	63	theme	in	213:214	arg1	biocompatibility					221:236	in vitro and in vivo biocompatibility	200:236	in vitro and in vivo biocompatibility	200:236	PURPOSE This study was designed to evaluate the in vitro and in vivo biocompatibility and osteointegration of plasma-sprayed hydroxyapatite (HA)-coated polyethylene terephthalate (PET) ligaments encapsulated with a simvastatin (SV)-chitosan (CS) composite.
31308664	6	64	theme	Sprague-Dawley	1000:1013	arg1	rats					1015:1018	male Sprague-Dawley rats	995:1018	male Sprague-Dawley rats	995:1018	Osteointegration was performed by implanting the PET ligaments into the proximal tibia bone tunnels of male Sprague-Dawley rats for 3 weeks and 6 weeks.
31308664	12	65	theme	PET	2244:2246	arg1	ligaments					2248:2256	plasma-sprayed HA-coated PET ligaments	2219:2256	plasma-sprayed HA-coated PET ligaments	2219:2256	CONCLUSION The SV-CS composite nanoporous structure was associated with the improved biocompatibility and osteogenic differentiation in vitro and enhanced osteointegration process in vivo of plasma-sprayed HA-coated PET ligaments.
31308664	3	66	theme	scanning	564:571	arg1	microscope					582:591	A field emission scanning electron microscope	547:591	A field emission scanning electron microscope	547:591	A field emission scanning electron microscope was used to characterize the morphology, and the in vitro SV release profile was analyzed.
31308664	8	67	theme	HA	1253:1254	arg1	particles					1256:1264	the plasma-sprayed HA particles	1234:1264	the plasma-sprayed HA particles	1234:1264	RESULTS The characteristic nanoporous structures mainly formed on the surface of the plasma-sprayed HA particles in the SV/CS/PET-HA and CS/PET-HA groups.
31308664	11	68	theme	bone	1853:1856	arg1	contact					1866:1872	bone implant contact and interface area	1853:1891	contact	1866:1872	The in vivo tests showed that both micro-CT analysis (bone mineral density and bone volume per total volume) and histological analysis (bone implant contact and interface area) revealed significantly higher peri-implant bone formation and less interface area in the SV/CS/PET-HA group than in the other groups.
31308664	7	69	theme	micro-computed	1089:1102	arg1	tomography					1104:1113	micro-computed tomography	1089:1113	micro-computed tomography (micro-CT)	1089:1124	The bone-implant interface was evaluated by micro-computed tomography (micro-CT) and histological analysis.
31308664	7	69	theme	micro-computed	1089:1102	arg1	micro-CT					1116:1123	micro-CT	1116:1123	micro-CT	1116:1123	The bone-implant interface was evaluated by micro-computed tomography (micro-CT) and histological analysis.
31308664	5	70	theme	marker	867:872	arg1	genes					874:878	marker genes	867:878	marker genes using qPCR	867:889	Osteogenic differentiation was investigated by the expression of marker genes using qPCR.
31308664	6	71	theme	bone	979:982	arg1	tunnels					984:990	the proximal tibia bone tunnels	960:990	the proximal tibia bone tunnels of male Sprague-Dawley rats for 3 weeks and 6 weeks	960:1042	Osteointegration was performed by implanting the PET ligaments into the proximal tibia bone tunnels of male Sprague-Dawley rats for 3 weeks and 6 weeks.
31308664	12	72	theme	composite	2049:2057	arg1	structure					2070:2078	The SV-CS composite nanoporous structure	2039:2078	The SV-CS composite nanoporous structure	2039:2078	CONCLUSION The SV-CS composite nanoporous structure was associated with the improved biocompatibility and osteogenic differentiation in vitro and enhanced osteointegration process in vivo of plasma-sprayed HA-coated PET ligaments.
31308664	10	73	theme	PET-HA	1693:1698	arg1	groups					1709:1714	the CS/PET-HA and PET-HA ligament groups	1675:1714	groups	1709:1714	The in vitro studies demonstrated that the SV/CS/PET-HA ligaments induced osteogenic differentiation in the MC3T3 cells, with higher mRNA expression levels of collagen-1, bone morphogenetic protein-2, osteocalcin and alkaline phosphatase than those in the CS/PET-HA and PET-HA ligament groups.
31308664	7	74	theme	bone-implant	1049:1060	arg1	interface					1062:1070	The bone-implant interface	1045:1070	The bone-implant interface	1045:1070	The bone-implant interface was evaluated by micro-computed tomography (micro-CT) and histological analysis.
31308664	8	75	theme	SV/CS/PET-HA	1273:1284	arg1	groups					1300:1305	the SV/CS/PET-HA and CS/PET-HA groups	1269:1305	the SV/CS/PET-HA and CS/PET-HA groups	1269:1305	RESULTS The characteristic nanoporous structures mainly formed on the surface of the plasma-sprayed HA particles in the SV/CS/PET-HA and CS/PET-HA groups.
31308664	6	76	theme	proximal	964:971	arg1	tunnels					984:990	the proximal tibia bone tunnels	960:990	the proximal tibia bone tunnels of male Sprague-Dawley rats for 3 weeks and 6 weeks	960:1042	Osteointegration was performed by implanting the PET ligaments into the proximal tibia bone tunnels of male Sprague-Dawley rats for 3 weeks and 6 weeks.
31308664	3	77	used	used	597:600	arg2	microscope					582:591	A field emission scanning electron microscope	547:591	A field emission scanning electron microscope	547:591	A field emission scanning electron microscope was used to characterize the morphology, and the in vitro SV release profile was analyzed.
31308664	11	78	theme	interface	1878:1886	arg1	area					1888:1891	bone implant contact and interface area	1853:1891	area	1888:1891	The in vivo tests showed that both micro-CT analysis (bone mineral density and bone volume per total volume) and histological analysis (bone implant contact and interface area) revealed significantly higher peri-implant bone formation and less interface area in the SV/CS/PET-HA group than in the other groups.
31308664	9	79	theme	release	1315:1321	arg1	test					1323:1326	The SV release test	1308:1326	The SV release test	1308:1326	The SV release test showed that the sustained release of simvastatin lasted for 25 days in the SV/CS/PET-HA group.
31308664	12	80	theme	enhanced	2174:2181	arg1	process					2200:2206	enhanced osteointegration process	2174:2206	enhanced osteointegration process in vivo of plasma-sprayed HA-coated PET ligaments	2174:2256	CONCLUSION The SV-CS composite nanoporous structure was associated with the improved biocompatibility and osteogenic differentiation in vitro and enhanced osteointegration process in vivo of plasma-sprayed HA-coated PET ligaments.
31308664	11	81	dep	analysis	1843:1850	arg1	contact					1866:1872	bone implant contact and interface area	1853:1891	contact	1866:1872	The in vivo tests showed that both micro-CT analysis (bone mineral density and bone volume per total volume) and histological analysis (bone implant contact and interface area) revealed significantly higher peri-implant bone formation and less interface area in the SV/CS/PET-HA group than in the other groups.
31308664	11	81	dep	analysis	1843:1850	arg1	area					1888:1891	bone implant contact and interface area	1853:1891	area	1888:1891	The in vivo tests showed that both micro-CT analysis (bone mineral density and bone volume per total volume) and histological analysis (bone implant contact and interface area) revealed significantly higher peri-implant bone formation and less interface area in the SV/CS/PET-HA group than in the other groups.
31308664	12	82	theme	SV-CS	2043:2047	arg1	structure					2070:2078	The SV-CS composite nanoporous structure	2039:2078	The SV-CS composite nanoporous structure	2039:2078	CONCLUSION The SV-CS composite nanoporous structure was associated with the improved biocompatibility and osteogenic differentiation in vitro and enhanced osteointegration process in vivo of plasma-sprayed HA-coated PET ligaments.
31308664	1	83	theme	plasma-sprayed	262:275	arg1	HA					293:294	HA	293:294	HA	293:294	PURPOSE This study was designed to evaluate the in vitro and in vivo biocompatibility and osteointegration of plasma-sprayed hydroxyapatite (HA)-coated polyethylene terephthalate (PET) ligaments encapsulated with a simvastatin (SV)-chitosan (CS) composite.
31308664	1	83	theme	plasma-sprayed	262:275	arg1	hydroxyapatite					277:290	plasma-sprayed hydroxyapatite	262:290	plasma-sprayed hydroxyapatite (HA)-coated polyethylene terephthalate (PET) ligaments encapsulated with a simvastatin (SV)-chitosan (CS) composite	262:406	PURPOSE This study was designed to evaluate the in vitro and in vivo biocompatibility and osteointegration of plasma-sprayed hydroxyapatite (HA)-coated polyethylene terephthalate (PET) ligaments encapsulated with a simvastatin (SV)-chitosan (CS) composite.
31308664	0	84	theme	polyethylene	114:125	arg1	ligaments					141:149	hydroxyapatite-coated polyethylene terephthalate ligaments	92:149	hydroxyapatite-coated polyethylene terephthalate ligaments	92:149	Encapsulation of a nanoporous simvastatin-chitosan composite to enhance osteointegration of hydroxyapatite-coated polyethylene terephthalate ligaments.
31308664	2	85	theme	PET	496:498	arg1	ligaments					500:508	three different PET ligaments	480:508	three different PET ligaments	480:508	METHODS This study compared the in vitro and in vivo bone responses to three different PET ligaments: SV/CS/PET-HA, CS/PET-HA and PET-HA.
31308664	0	86	theme	ligaments	141:149	arg1	osteointegration					72:87	osteointegration	72:87	osteointegration of hydroxyapatite-coated polyethylene terephthalate ligaments	72:149	Encapsulation of a nanoporous simvastatin-chitosan composite to enhance osteointegration of hydroxyapatite-coated polyethylene terephthalate ligaments.
31308664	1	87	theme	-chitosan	383:391	arg1	composite					398:406	a simvastatin (SV)-chitosan (CS) composite	365:406	a simvastatin (SV)-chitosan (CS) composite	365:406	PURPOSE This study was designed to evaluate the in vitro and in vivo biocompatibility and osteointegration of plasma-sprayed hydroxyapatite (HA)-coated polyethylene terephthalate (PET) ligaments encapsulated with a simvastatin (SV)-chitosan (CS) composite.
31308664	10	88	theme	osteogenic	1497:1506	arg1	differentiation					1508:1522	osteogenic differentiation	1497:1522	osteogenic differentiation	1497:1522	The in vitro studies demonstrated that the SV/CS/PET-HA ligaments induced osteogenic differentiation in the MC3T3 cells, with higher mRNA expression levels of collagen-1, bone morphogenetic protein-2, osteocalcin and alkaline phosphatase than those in the CS/PET-HA and PET-HA ligament groups.
31308664	9	89	theme	simvastatin	1365:1375	arg1	release					1354:1360	the sustained release	1340:1360	the sustained release of simvastatin	1340:1375	The SV release test showed that the sustained release of simvastatin lasted for 25 days in the SV/CS/PET-HA group.
31308664	2	90	dep	in	454:455	arg1	vivo					457:460	vivo	457:460	vivo	457:460	METHODS This study compared the in vitro and in vivo bone responses to three different PET ligaments: SV/CS/PET-HA, CS/PET-HA and PET-HA.
31308664	11	91	dep	analysis	1761:1768	arg1	density					1784:1790	bone mineral density	1771:1790	bone mineral density	1771:1790	The in vivo tests showed that both micro-CT analysis (bone mineral density and bone volume per total volume) and histological analysis (bone implant contact and interface area) revealed significantly higher peri-implant bone formation and less interface area in the SV/CS/PET-HA group than in the other groups.
31308664	11	91	dep	analysis	1761:1768	arg1	volume					1801:1806	bone volume	1796:1806	bone volume	1796:1806	The in vivo tests showed that both micro-CT analysis (bone mineral density and bone volume per total volume) and histological analysis (bone implant contact and interface area) revealed significantly higher peri-implant bone formation and less interface area in the SV/CS/PET-HA group than in the other groups.
31308664	10	92	theme	MC3T3	1531:1535	arg1	cells					1537:1541	the MC3T3 cells	1527:1541	the MC3T3 cells	1527:1541	The in vitro studies demonstrated that the SV/CS/PET-HA ligaments induced osteogenic differentiation in the MC3T3 cells, with higher mRNA expression levels of collagen-1, bone morphogenetic protein-2, osteocalcin and alkaline phosphatase than those in the CS/PET-HA and PET-HA ligament groups.
31308664	1	93	dep	in	200:201	arg1	vitro					203:207	vitro	203:207	vitro	203:207	PURPOSE This study was designed to evaluate the in vitro and in vivo biocompatibility and osteointegration of plasma-sprayed hydroxyapatite (HA)-coated polyethylene terephthalate (PET) ligaments encapsulated with a simvastatin (SV)-chitosan (CS) composite.
31308664	0	94	theme	nanoporous	19:28	arg1	composite					51:59	a nanoporous simvastatin-chitosan composite	17:59	a nanoporous simvastatin-chitosan composite	17:59	Encapsulation of a nanoporous simvastatin-chitosan composite to enhance osteointegration of hydroxyapatite-coated polyethylene terephthalate ligaments.
31308664	1	95	theme	PET	332:334	arg1	ligaments					337:345	plasma-sprayed hydroxyapatite (HA)-coated polyethylene terephthalate (PET) ligaments	262:345	plasma-sprayed hydroxyapatite (HA)-coated polyethylene terephthalate (PET) ligaments encapsulated with a simvastatin (SV)-chitosan (CS) composite	262:406	PURPOSE This study was designed to evaluate the in vitro and in vivo biocompatibility and osteointegration of plasma-sprayed hydroxyapatite (HA)-coated polyethylene terephthalate (PET) ligaments encapsulated with a simvastatin (SV)-chitosan (CS) composite.
31308664	10	96	theme	expression	1561:1570	arg1	levels					1572:1577	higher mRNA expression levels	1549:1577	higher mRNA expression levels of collagen-1, bone morphogenetic protein-2, osteocalcin and alkaline phosphatase than those in the CS/PET-HA and PET-HA ligament groups	1549:1714	The in vitro studies demonstrated that the SV/CS/PET-HA ligaments induced osteogenic differentiation in the MC3T3 cells, with higher mRNA expression levels of collagen-1, bone morphogenetic protein-2, osteocalcin and alkaline phosphatase than those in the CS/PET-HA and PET-HA ligament groups.
31308664	3	97	theme	SV	651:652	arg1	profile					662:668	the in vitro SV release profile	638:668	the in vitro SV release profile	638:668	A field emission scanning electron microscope was used to characterize the morphology, and the in vitro SV release profile was analyzed.
31308664	2	98	theme	in	441:442	arg1	responses					467:475	the in vitro and in vivo bone responses	437:475	the in vitro and in vivo bone responses to three different PET ligaments	437:508	METHODS This study compared the in vitro and in vivo bone responses to three different PET ligaments: SV/CS/PET-HA, CS/PET-HA and PET-HA.
31308664	0	99	theme	composite	51:59	arg1	Encapsulation					0:12	Encapsulation	0:12	Encapsulation of a nanoporous simvastatin-chitosan composite	0:59	Encapsulation of a nanoporous simvastatin-chitosan composite to enhance osteointegration of hydroxyapatite-coated polyethylene terephthalate ligaments.
31308664	1	100	theme	ligaments	337:345	arg1	osteointegration					242:257	osteointegration	242:257	osteointegration	242:257	PURPOSE This study was designed to evaluate the in vitro and in vivo biocompatibility and osteointegration of plasma-sprayed hydroxyapatite (HA)-coated polyethylene terephthalate (PET) ligaments encapsulated with a simvastatin (SV)-chitosan (CS) composite.
31308664	1	100	theme	ligaments	337:345	arg1	biocompatibility					221:236	in vitro and in vivo biocompatibility	200:236	in vitro and in vivo biocompatibility	200:236	PURPOSE This study was designed to evaluate the in vitro and in vivo biocompatibility and osteointegration of plasma-sprayed hydroxyapatite (HA)-coated polyethylene terephthalate (PET) ligaments encapsulated with a simvastatin (SV)-chitosan (CS) composite.
31308664	10	101	theme	higher	1549:1554	arg1	levels					1572:1577	higher mRNA expression levels	1549:1577	higher mRNA expression levels of collagen-1, bone morphogenetic protein-2, osteocalcin and alkaline phosphatase than those in the CS/PET-HA and PET-HA ligament groups	1549:1714	The in vitro studies demonstrated that the SV/CS/PET-HA ligaments induced osteogenic differentiation in the MC3T3 cells, with higher mRNA expression levels of collagen-1, bone morphogenetic protein-2, osteocalcin and alkaline phosphatase than those in the CS/PET-HA and PET-HA ligament groups.
31308664	11	102	theme	micro-CT	1752:1759	arg1	analysis					1761:1768	micro-CT analysis	1752:1768	micro-CT analysis (bone mineral density and bone volume per total volume)	1752:1824	The in vivo tests showed that both micro-CT analysis (bone mineral density and bone volume per total volume) and histological analysis (bone implant contact and interface area) revealed significantly higher peri-implant bone formation and less interface area in the SV/CS/PET-HA group than in the other groups.
31308664	5	103	theme	Osteogenic	802:811	arg1	differentiation					813:827	Osteogenic differentiation	802:827	Osteogenic differentiation	802:827	Osteogenic differentiation was investigated by the expression of marker genes using qPCR.
31308664	1	104	dep	PURPOSE	152:158	arg1	designed					175:182	designed	175:182	was designed to evaluate the in vitro and in vivo biocompatibility and osteointegration of plasma-sprayed hydroxyapatite (HA)-coated polyethylene terephthalate (PET) ligaments encapsulated with a simvastatin (SV)-chitosan (CS) composite	171:406	PURPOSE This study was designed to evaluate the in vitro and in vivo biocompatibility and osteointegration of plasma-sprayed hydroxyapatite (HA)-coated polyethylene terephthalate (PET) ligaments encapsulated with a simvastatin (SV)-chitosan (CS) composite.
31308664	2	105	dep	in	441:442	arg1	vitro					444:448	vitro	444:448	vitro	444:448	METHODS This study compared the in vitro and in vivo bone responses to three different PET ligaments: SV/CS/PET-HA, CS/PET-HA and PET-HA.
31308664	11	106	theme	peri-implant	1924:1935	arg1	formation					1942:1950	significantly higher peri-implant bone formation	1903:1950	significantly higher peri-implant bone formation	1903:1950	The in vivo tests showed that both micro-CT analysis (bone mineral density and bone volume per total volume) and histological analysis (bone implant contact and interface area) revealed significantly higher peri-implant bone formation and less interface area in the SV/CS/PET-HA group than in the other groups.
31308664	8	107	theme	characteristic	1165:1178	arg1	structures					1191:1200	The characteristic nanoporous structures	1161:1200	The characteristic nanoporous structures mainly formed on the surface of the plasma-sprayed HA particles in the SV/CS/PET-HA and CS/PET-HA groups	1161:1305	RESULTS The characteristic nanoporous structures mainly formed on the surface of the plasma-sprayed HA particles in the SV/CS/PET-HA and CS/PET-HA groups.
31308664	11	108	theme	mineral	1776:1782	arg1	density					1784:1790	bone mineral density	1771:1790	bone mineral density	1771:1790	The in vivo tests showed that both micro-CT analysis (bone mineral density and bone volume per total volume) and histological analysis (bone implant contact and interface area) revealed significantly higher peri-implant bone formation and less interface area in the SV/CS/PET-HA group than in the other groups.
31308664	10	109	theme	in	1427:1428	arg1	studies					1436:1442	The in vitro studies	1423:1442	The in vitro studies	1423:1442	The in vitro studies demonstrated that the SV/CS/PET-HA ligaments induced osteogenic differentiation in the MC3T3 cells, with higher mRNA expression levels of collagen-1, bone morphogenetic protein-2, osteocalcin and alkaline phosphatase than those in the CS/PET-HA and PET-HA ligament groups.
31308664	1	110	theme	hydroxyapatite	277:290	arg1	ligaments					337:345	plasma-sprayed hydroxyapatite (HA)-coated polyethylene terephthalate (PET) ligaments	262:345	plasma-sprayed hydroxyapatite (HA)-coated polyethylene terephthalate (PET) ligaments encapsulated with a simvastatin (SV)-chitosan (CS) composite	262:406	PURPOSE This study was designed to evaluate the in vitro and in vivo biocompatibility and osteointegration of plasma-sprayed hydroxyapatite (HA)-coated polyethylene terephthalate (PET) ligaments encapsulated with a simvastatin (SV)-chitosan (CS) composite.
31308664	11	111	theme	in	1721:1722	arg1	tests					1729:1733	The in vivo tests	1717:1733	The in vivo tests	1717:1733	The in vivo tests showed that both micro-CT analysis (bone mineral density and bone volume per total volume) and histological analysis (bone implant contact and interface area) revealed significantly higher peri-implant bone formation and less interface area in the SV/CS/PET-HA group than in the other groups.
30311249	0	0	theme	nanofibrous	71:81	arg1	mat					83:85	a nanofibrous mat	69:85	a nanofibrous mat	69:85	Application of polycaprolactone, chitosan, and collagen composite as a nanofibrous mat loaded with silver sulfadiazine and growth factors for wound dressing.
30311249	1	1	with	materials	232:240	arg1	manner					319:324	controlled release manner	300:324	controlled release manner	300:324	Fabrication of nanofibrous biomaterials composed of natural and synthetic materials that incorporated with antibiotic and growth factors with controlled release manner is an attractive topic in wound healing.
30311249	7	2	theme	Nanofibrous	1140:1150	arg1	dressings					1210:1218	wound dressings	1204:1218	wound dressings	1204:1218	Nanofibrous mats with or without growth factors were applied as wound dressings and at day 14, the healing process was evaluated.
30311249	7	2	theme	Nanofibrous	1140:1150	arg1	mats					1152:1155	Nanofibrous mats	1140:1155	Nanofibrous mats with or without growth factors	1140:1186	Nanofibrous mats with or without growth factors were applied as wound dressings and at day 14, the healing process was evaluated.
30311249	2	3	theme	wound	489:493	arg1	healing					495:501	wound healing	489:501	wound healing	489:501	The purpose of this study was to prepare optimal composite of materials as biomimetic nanofibrous mats for application in wound healing.
30311249	1	4	theme	wound	352:356	arg1	healing					358:364	wound healing	352:364	wound healing	352:364	Fabrication of nanofibrous biomaterials composed of natural and synthetic materials that incorporated with antibiotic and growth factors with controlled release manner is an attractive topic in wound healing.
30311249	9	5	from	desirable	1401:1409	arg1	closure					1420:1426	wound closure	1414:1426	wound closure	1414:1426	Silicone ring in the test group was desirable in wound closure compared to the control group.
30311249	3	6	theme	top	654:656	arg1	layer					658:662	the top layer	650:662	the top layer	650:662	The mat was prepared of polycaprolactone (PCL) in the bottom, chitosan/poly ethylene oxide (Cs/PEO) in the middle, and PCL/collagen (PCL/Coll) in the top layer.
30311249	6	7	theme	tissue	1119:1124	arg1	contractions					1126:1137	the usual tissue contractions	1109:1137	the usual tissue contractions	1109:1137	Then, on the dorsum of rats, a 400-mm2 wound was created and surrounded by a silicone ring to control the usual tissue contractions.
30311249	6	8	theme	silicone	1084:1091	arg1	ring					1093:1096	a silicone ring	1082:1096	a silicone ring	1082:1096	Then, on the dorsum of rats, a 400-mm2 wound was created and surrounded by a silicone ring to control the usual tissue contractions.
30311249	9	9	from	closure	1420:1426	arg1	desirable					1401:1409	desirable	1401:1409	desirable	1401:1409	Silicone ring in the test group was desirable in wound closure compared to the control group.
30311249	11	10	theme	effective	1588:1596	arg1	mat					1547:1549	This composite nanofibrous mat	1520:1549	This composite nanofibrous mat	1520:1549	This composite nanofibrous mat could be introduced as a dynamic and effective candidate for wound dressing.
30311249	11	10	theme	effective	1588:1596	arg1	candidate					1598:1606	a dynamic and effective candidate	1574:1606	a dynamic and effective candidate for wound dressing	1574:1625	This composite nanofibrous mat could be introduced as a dynamic and effective candidate for wound dressing.
30311249	4	11	theme	mat	723:725	arg1	tests					702:706	standard characterization tests	676:706	standard characterization tests of nanofibrous mat	676:725	A panel of standard characterization tests of nanofibrous mat was performed and its compatibilities in strength and integration were confirmed.
30311249	1	12	theme	growth	280:285	arg1	factors					287:293	growth factors	280:293	growth factors	280:293	Fabrication of nanofibrous biomaterials composed of natural and synthetic materials that incorporated with antibiotic and growth factors with controlled release manner is an attractive topic in wound healing.
30311249	0	13	theme	silver	99:104	arg1	sulfadiazine					106:117	silver sulfadiazine	99:117	silver sulfadiazine	99:117	Application of polycaprolactone, chitosan, and collagen composite as a nanofibrous mat loaded with silver sulfadiazine and growth factors for wound dressing.
30311249	5	14	theme	silver	915:920	arg1	SSD					936:938	SSD	936:938	SSD	936:938	Middle layer was loaded with epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF), and silver sulfadiazine (SSD) was incorporated in the bottom layer as an anti-infection factor.
30311249	5	14	theme	silver	915:920	arg1	factor					999:1004	an anti-infection factor	981:1004	an anti-infection factor	981:1004	Middle layer was loaded with epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF), and silver sulfadiazine (SSD) was incorporated in the bottom layer as an anti-infection factor.
30311249	5	14	theme	silver	915:920	arg1	sulfadiazine					922:933	silver sulfadiazine	915:933	silver sulfadiazine (SSD)	915:939	Middle layer was loaded with epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF), and silver sulfadiazine (SSD) was incorporated in the bottom layer as an anti-infection factor.
30311249	2	15	theme	study	387:391	arg1	purpose					371:377	The purpose	367:377	The purpose of this study	367:391	The purpose of this study was to prepare optimal composite of materials as biomimetic nanofibrous mats for application in wound healing.
30311249	3	16	from	PCL/collagen	623:634	arg1	layer					658:662	the top layer	650:662	the top layer	650:662	The mat was prepared of polycaprolactone (PCL) in the bottom, chitosan/poly ethylene oxide (Cs/PEO) in the middle, and PCL/collagen (PCL/Coll) in the top layer.
30311249	3	16	from	PCL/collagen	623:634	arg1	middle					611:616	the middle	607:616	the middle	607:616	The mat was prepared of polycaprolactone (PCL) in the bottom, chitosan/poly ethylene oxide (Cs/PEO) in the middle, and PCL/collagen (PCL/Coll) in the top layer.
30311249	3	16	from	PCL/collagen	623:634	arg1	bottom					558:563	the bottom	554:563	the bottom	554:563	The mat was prepared of polycaprolactone (PCL) in the bottom, chitosan/poly ethylene oxide (Cs/PEO) in the middle, and PCL/collagen (PCL/Coll) in the top layer.
30311249	11	17	theme	wound	1612:1616	arg1	dressing					1618:1625	wound dressing	1612:1625	wound dressing	1612:1625	This composite nanofibrous mat could be introduced as a dynamic and effective candidate for wound dressing.
30311249	10	18	theme	skin	1474:1477	arg1	tissue					1479:1484	skin tissue	1474:1484	skin tissue	1474:1484	Reformation of skin tissue was manifested in a shorter time.
30311249	10	19	theme	shorter	1506:1512	arg1	time					1514:1517	a shorter time	1504:1517	a shorter time	1504:1517	Reformation of skin tissue was manifested in a shorter time.
30311249	2	20	theme	nanofibrous	453:463	arg1	materials					429:437	materials	429:437	materials	429:437	The purpose of this study was to prepare optimal composite of materials as biomimetic nanofibrous mats for application in wound healing.
30311249	2	20	theme	nanofibrous	453:463	arg1	composite					416:424	optimal composite	408:424	optimal composite of materials	408:437	The purpose of this study was to prepare optimal composite of materials as biomimetic nanofibrous mats for application in wound healing.
30311249	2	20	theme	nanofibrous	453:463	arg1	mats					465:468	biomimetic nanofibrous mats	442:468	biomimetic nanofibrous mats for application in wound healing	442:501	The purpose of this study was to prepare optimal composite of materials as biomimetic nanofibrous mats for application in wound healing.
30311249	2	21	from	application	474:484	arg1	healing					495:501	wound healing	489:501	wound healing	489:501	The purpose of this study was to prepare optimal composite of materials as biomimetic nanofibrous mats for application in wound healing.
30311249	4	22	theme	tests	702:706	arg1	panel					667:671	A panel	665:671	A panel of standard characterization tests of nanofibrous mat	665:725	A panel of standard characterization tests of nanofibrous mat was performed and its compatibilities in strength and integration were confirmed.
30311249	7	23	theme	growth	1173:1178	arg1	factors					1180:1186	growth factors	1173:1186	growth factors	1173:1186	Nanofibrous mats with or without growth factors were applied as wound dressings and at day 14, the healing process was evaluated.
30311249	1	24	theme	nanofibrous	173:183	arg1	biomaterials					185:196	nanofibrous biomaterials	173:196	nanofibrous biomaterials	173:196	Fabrication of nanofibrous biomaterials composed of natural and synthetic materials that incorporated with antibiotic and growth factors with controlled release manner is an attractive topic in wound healing.
30311249	2	25	theme	biomimetic	442:451	arg1	materials					429:437	materials	429:437	materials	429:437	The purpose of this study was to prepare optimal composite of materials as biomimetic nanofibrous mats for application in wound healing.
30311249	2	25	theme	biomimetic	442:451	arg1	composite					416:424	optimal composite	408:424	optimal composite of materials	408:437	The purpose of this study was to prepare optimal composite of materials as biomimetic nanofibrous mats for application in wound healing.
30311249	2	25	theme	biomimetic	442:451	arg1	mats					465:468	biomimetic nanofibrous mats	442:468	biomimetic nanofibrous mats for application in wound healing	442:501	The purpose of this study was to prepare optimal composite of materials as biomimetic nanofibrous mats for application in wound healing.
30311249	1	26	with	factors	287:293	arg1	manner					319:324	controlled release manner	300:324	controlled release manner	300:324	Fabrication of nanofibrous biomaterials composed of natural and synthetic materials that incorporated with antibiotic and growth factors with controlled release manner is an attractive topic in wound healing.
30311249	4	27	theme	characterization	685:700	arg1	tests					702:706	standard characterization tests	676:706	standard characterization tests of nanofibrous mat	676:725	A panel of standard characterization tests of nanofibrous mat was performed and its compatibilities in strength and integration were confirmed.
30311249	1	28	theme	biomaterials	185:196	arg1	Fabrication					158:168	Fabrication	158:168	Fabrication of nanofibrous biomaterials composed of natural and synthetic materials that incorporated with antibiotic and growth factors with controlled release manner	158:324	Fabrication of nanofibrous biomaterials composed of natural and synthetic materials that incorporated with antibiotic and growth factors with controlled release manner is an attractive topic in wound healing.
30311249	1	28	theme	biomaterials	185:196	arg1	topic					343:347	an attractive topic	329:347	an attractive topic in wound healing	329:364	Fabrication of nanofibrous biomaterials composed of natural and synthetic materials that incorporated with antibiotic and growth factors with controlled release manner is an attractive topic in wound healing.
30311249	9	29	from	ring	1374:1377	arg1	group					1391:1395	the test group	1382:1395	the test group	1382:1395	Silicone ring in the test group was desirable in wound closure compared to the control group.
30311249	5	30	theme	bottom	965:970	arg1	layer					972:976	the bottom layer	961:976	the bottom layer	961:976	Middle layer was loaded with epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF), and silver sulfadiazine (SSD) was incorporated in the bottom layer as an anti-infection factor.
30311249	4	31	theme	nanofibrous	711:721	arg1	mat					723:725	nanofibrous mat	711:725	nanofibrous mat	711:725	A panel of standard characterization tests of nanofibrous mat was performed and its compatibilities in strength and integration were confirmed.
30311249	7	32	theme	wound	1204:1208	arg1	dressings					1210:1218	wound dressings	1204:1218	wound dressings	1204:1218	Nanofibrous mats with or without growth factors were applied as wound dressings and at day 14, the healing process was evaluated.
30311249	7	32	theme	wound	1204:1208	arg1	mats					1152:1155	Nanofibrous mats	1140:1155	Nanofibrous mats with or without growth factors	1140:1186	Nanofibrous mats with or without growth factors were applied as wound dressings and at day 14, the healing process was evaluated.
30311249	2	33	theme	materials	429:437	arg1	materials					429:437	materials	429:437	materials	429:437	The purpose of this study was to prepare optimal composite of materials as biomimetic nanofibrous mats for application in wound healing.
30311249	2	33	theme	materials	429:437	arg1	composite					416:424	optimal composite	408:424	optimal composite of materials	408:437	The purpose of this study was to prepare optimal composite of materials as biomimetic nanofibrous mats for application in wound healing.
30311249	2	33	theme	materials	429:437	arg1	mats					465:468	biomimetic nanofibrous mats	442:468	biomimetic nanofibrous mats for application in wound healing	442:501	The purpose of this study was to prepare optimal composite of materials as biomimetic nanofibrous mats for application in wound healing.
30311249	1	34	theme	controlled	300:309	arg1	manner					319:324	controlled release manner	300:324	controlled release manner	300:324	Fabrication of nanofibrous biomaterials composed of natural and synthetic materials that incorporated with antibiotic and growth factors with controlled release manner is an attractive topic in wound healing.
30311249	9	35	theme	control	1444:1450	arg1	group					1452:1456	the control group	1440:1456	the control group	1440:1456	Silicone ring in the test group was desirable in wound closure compared to the control group.
30311249	3	36	from	oxide	589:593	arg1	layer					658:662	the top layer	650:662	the top layer	650:662	The mat was prepared of polycaprolactone (PCL) in the bottom, chitosan/poly ethylene oxide (Cs/PEO) in the middle, and PCL/collagen (PCL/Coll) in the top layer.
30311249	3	36	from	oxide	589:593	arg1	middle					611:616	the middle	607:616	the middle	607:616	The mat was prepared of polycaprolactone (PCL) in the bottom, chitosan/poly ethylene oxide (Cs/PEO) in the middle, and PCL/collagen (PCL/Coll) in the top layer.
30311249	3	36	from	oxide	589:593	arg1	bottom					558:563	the bottom	554:563	the bottom	554:563	The mat was prepared of polycaprolactone (PCL) in the bottom, chitosan/poly ethylene oxide (Cs/PEO) in the middle, and PCL/collagen (PCL/Coll) in the top layer.
30311249	5	37	theme	Middle	809:814	arg1	layer					816:820	Middle layer	809:820	Middle layer	809:820	Middle layer was loaded with epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF), and silver sulfadiazine (SSD) was incorporated in the bottom layer as an anti-infection factor.
30311249	1	38	theme	release	311:317	arg1	manner					319:324	controlled release manner	300:324	controlled release manner	300:324	Fabrication of nanofibrous biomaterials composed of natural and synthetic materials that incorporated with antibiotic and growth factors with controlled release manner is an attractive topic in wound healing.
30311249	2	39	theme	optimal	408:414	arg1	materials					429:437	materials	429:437	materials	429:437	The purpose of this study was to prepare optimal composite of materials as biomimetic nanofibrous mats for application in wound healing.
30311249	2	39	theme	optimal	408:414	arg1	composite					416:424	optimal composite	408:424	optimal composite of materials	408:437	The purpose of this study was to prepare optimal composite of materials as biomimetic nanofibrous mats for application in wound healing.
30311249	2	39	theme	optimal	408:414	arg1	mats					465:468	biomimetic nanofibrous mats	442:468	biomimetic nanofibrous mats for application in wound healing	442:501	The purpose of this study was to prepare optimal composite of materials as biomimetic nanofibrous mats for application in wound healing.
30311249	5	40	theme	growth	848:853	arg1	EGF					863:865	EGF	863:865	EGF	863:865	Middle layer was loaded with epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF), and silver sulfadiazine (SSD) was incorporated in the bottom layer as an anti-infection factor.
30311249	5	40	theme	growth	848:853	arg1	factor					855:860	epidermal growth factor	838:860	epidermal growth factor (EGF)	838:866	Middle layer was loaded with epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF), and silver sulfadiazine (SSD) was incorporated in the bottom layer as an anti-infection factor.
30311249	1	41	theme	natural	210:216	arg1	materials					232:240	natural and synthetic materials	210:240	natural and synthetic materials that incorporated with antibiotic	210:274	Fabrication of nanofibrous biomaterials composed of natural and synthetic materials that incorporated with antibiotic and growth factors with controlled release manner is an attractive topic in wound healing.
30311249	0	42	theme	chitosan	33:40	arg1	Application					0:10	Application	0:10	Application of polycaprolactone, chitosan, and collagen composite as a nanofibrous mat	0:85	Application of polycaprolactone, chitosan, and collagen composite as a nanofibrous mat loaded with silver sulfadiazine and growth factors for wound dressing.
30311249	0	43	theme	growth	123:128	arg1	factors					130:136	growth factors	123:136	growth factors for wound dressing	123:155	Application of polycaprolactone, chitosan, and collagen composite as a nanofibrous mat loaded with silver sulfadiazine and growth factors for wound dressing.
30311249	9	44	theme	Silicone	1365:1372	arg1	ring					1374:1377	Silicone ring	1365:1377	Silicone ring in the test group	1365:1395	Silicone ring in the test group was desirable in wound closure compared to the control group.
30311249	0	45	theme	collagen	47:54	arg1	Application					0:10	Application	0:10	Application of polycaprolactone, chitosan, and collagen composite as a nanofibrous mat	0:85	Application of polycaprolactone, chitosan, and collagen composite as a nanofibrous mat loaded with silver sulfadiazine and growth factors for wound dressing.
30311249	5	46	theme	epidermal	838:846	arg1	EGF					863:865	EGF	863:865	EGF	863:865	Middle layer was loaded with epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF), and silver sulfadiazine (SSD) was incorporated in the bottom layer as an anti-infection factor.
30311249	5	46	theme	epidermal	838:846	arg1	factor					855:860	epidermal growth factor	838:860	epidermal growth factor (EGF)	838:866	Middle layer was loaded with epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF), and silver sulfadiazine (SSD) was incorporated in the bottom layer as an anti-infection factor.
30311249	0	47	theme	wound	142:146	arg1	dressing					148:155	wound dressing	142:155	wound dressing	142:155	Application of polycaprolactone, chitosan, and collagen composite as a nanofibrous mat loaded with silver sulfadiazine and growth factors for wound dressing.
30311249	11	48	theme	nanofibrous	1535:1545	arg1	mat					1547:1549	This composite nanofibrous mat	1520:1549	This composite nanofibrous mat	1520:1549	This composite nanofibrous mat could be introduced as a dynamic and effective candidate for wound dressing.
30311249	11	48	theme	nanofibrous	1535:1545	arg1	candidate					1598:1606	a dynamic and effective candidate	1574:1606	a dynamic and effective candidate for wound dressing	1574:1625	This composite nanofibrous mat could be introduced as a dynamic and effective candidate for wound dressing.
30311249	3	49	theme	chitosan/poly	566:578	arg1	oxide					589:593	chitosan/poly ethylene oxide	566:593	chitosan/poly ethylene oxide (Cs/PEO) in the middle	566:616	The mat was prepared of polycaprolactone (PCL) in the bottom, chitosan/poly ethylene oxide (Cs/PEO) in the middle, and PCL/collagen (PCL/Coll) in the top layer.
30311249	3	49	theme	chitosan/poly	566:578	arg1	Cs/PEO					596:601	Cs/PEO	596:601	Cs/PEO	596:601	The mat was prepared of polycaprolactone (PCL) in the bottom, chitosan/poly ethylene oxide (Cs/PEO) in the middle, and PCL/collagen (PCL/Coll) in the top layer.
30311249	11	50	theme	composite	1525:1533	arg1	mat					1547:1549	This composite nanofibrous mat	1520:1549	This composite nanofibrous mat	1520:1549	This composite nanofibrous mat could be introduced as a dynamic and effective candidate for wound dressing.
30311249	11	50	theme	composite	1525:1533	arg1	candidate					1598:1606	a dynamic and effective candidate	1574:1606	a dynamic and effective candidate for wound dressing	1574:1625	This composite nanofibrous mat could be introduced as a dynamic and effective candidate for wound dressing.
30311249	6	51	theme	400-mm2	1038:1044	arg1	wound					1046:1050	a 400-mm2 wound	1036:1050	a 400-mm2 wound	1036:1050	Then, on the dorsum of rats, a 400-mm2 wound was created and surrounded by a silicone ring to control the usual tissue contractions.
30311249	9	52	theme	test	1386:1389	arg1	group					1391:1395	the test group	1382:1395	the test group	1382:1395	Silicone ring in the test group was desirable in wound closure compared to the control group.
30311249	9	53	theme	wound	1414:1418	arg1	closure					1420:1426	wound closure	1414:1426	wound closure	1414:1426	Silicone ring in the test group was desirable in wound closure compared to the control group.
30311249	3	54	theme	ethylene	580:587	arg1	oxide					589:593	chitosan/poly ethylene oxide	566:593	chitosan/poly ethylene oxide (Cs/PEO) in the middle	566:616	The mat was prepared of polycaprolactone (PCL) in the bottom, chitosan/poly ethylene oxide (Cs/PEO) in the middle, and PCL/collagen (PCL/Coll) in the top layer.
30311249	3	54	theme	ethylene	580:587	arg1	Cs/PEO					596:601	Cs/PEO	596:601	Cs/PEO	596:601	The mat was prepared of polycaprolactone (PCL) in the bottom, chitosan/poly ethylene oxide (Cs/PEO) in the middle, and PCL/collagen (PCL/Coll) in the top layer.
30311249	5	55	theme	basic	872:876	arg1	bFGF					904:907	bFGF	904:907	bFGF	904:907	Middle layer was loaded with epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF), and silver sulfadiazine (SSD) was incorporated in the bottom layer as an anti-infection factor.
30311249	5	55	theme	basic	872:876	arg1	factor					896:901	basic fibroblast growth factor	872:901	basic fibroblast growth factor (bFGF)	872:908	Middle layer was loaded with epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF), and silver sulfadiazine (SSD) was incorporated in the bottom layer as an anti-infection factor.
30311249	6	56	theme	usual	1113:1117	arg1	contractions					1126:1137	the usual tissue contractions	1109:1137	the usual tissue contractions	1109:1137	Then, on the dorsum of rats, a 400-mm2 wound was created and surrounded by a silicone ring to control the usual tissue contractions.
30311249	1	57	theme	attractive	332:341	arg1	topic					343:347	an attractive topic	329:347	an attractive topic in wound healing	329:364	Fabrication of nanofibrous biomaterials composed of natural and synthetic materials that incorporated with antibiotic and growth factors with controlled release manner is an attractive topic in wound healing.
30311249	1	57	theme	attractive	332:341	arg1	Fabrication					158:168	Fabrication	158:168	Fabrication of nanofibrous biomaterials composed of natural and synthetic materials that incorporated with antibiotic and growth factors with controlled release manner	158:324	Fabrication of nanofibrous biomaterials composed of natural and synthetic materials that incorporated with antibiotic and growth factors with controlled release manner is an attractive topic in wound healing.
30311249	7	58	theme	healing	1239:1245	arg1	process					1247:1253	the healing process	1235:1253	the healing process	1235:1253	Nanofibrous mats with or without growth factors were applied as wound dressings and at day 14, the healing process was evaluated.
30311249	4	59	theme	standard	676:683	arg1	tests					702:706	standard characterization tests	676:706	standard characterization tests of nanofibrous mat	676:725	A panel of standard characterization tests of nanofibrous mat was performed and its compatibilities in strength and integration were confirmed.
30311249	6	60	theme	rats	1030:1033	arg1	dorsum					1020:1025	the dorsum	1016:1025	the dorsum of rats	1016:1033	Then, on the dorsum of rats, a 400-mm2 wound was created and surrounded by a silicone ring to control the usual tissue contractions.
30311249	1	61	theme	synthetic	222:230	arg1	materials					232:240	natural and synthetic materials	210:240	natural and synthetic materials that incorporated with antibiotic	210:274	Fabrication of nanofibrous biomaterials composed of natural and synthetic materials that incorporated with antibiotic and growth factors with controlled release manner is an attractive topic in wound healing.
30311249	11	62	theme	dynamic	1576:1582	arg1	mat					1547:1549	This composite nanofibrous mat	1520:1549	This composite nanofibrous mat	1520:1549	This composite nanofibrous mat could be introduced as a dynamic and effective candidate for wound dressing.
30311249	11	62	theme	dynamic	1576:1582	arg1	candidate					1598:1606	a dynamic and effective candidate	1574:1606	a dynamic and effective candidate for wound dressing	1574:1625	This composite nanofibrous mat could be introduced as a dynamic and effective candidate for wound dressing.
30311249	8	63	theme	treated	1285:1291	arg1	group					1293:1297	the treated group	1281:1297	the treated group by designed mat	1281:1313	At day 14, the treated group by designed mat showed faster epithelialization and angiogenesis.
30311249	5	64	theme	growth	889:894	arg1	bFGF					904:907	bFGF	904:907	bFGF	904:907	Middle layer was loaded with epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF), and silver sulfadiazine (SSD) was incorporated in the bottom layer as an anti-infection factor.
30311249	5	64	theme	growth	889:894	arg1	factor					896:901	basic fibroblast growth factor	872:901	basic fibroblast growth factor (bFGF)	872:908	Middle layer was loaded with epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF), and silver sulfadiazine (SSD) was incorporated in the bottom layer as an anti-infection factor.
30311249	5	65	theme	fibroblast	878:887	arg1	bFGF					904:907	bFGF	904:907	bFGF	904:907	Middle layer was loaded with epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF), and silver sulfadiazine (SSD) was incorporated in the bottom layer as an anti-infection factor.
30311249	5	65	theme	fibroblast	878:887	arg1	factor					896:901	basic fibroblast growth factor	872:901	basic fibroblast growth factor (bFGF)	872:908	Middle layer was loaded with epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF), and silver sulfadiazine (SSD) was incorporated in the bottom layer as an anti-infection factor.
30311249	5	66	theme	anti-infection	984:997	arg1	factor					999:1004	an anti-infection factor	981:1004	an anti-infection factor	981:1004	Middle layer was loaded with epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF), and silver sulfadiazine (SSD) was incorporated in the bottom layer as an anti-infection factor.
30311249	5	66	theme	anti-infection	984:997	arg1	sulfadiazine					922:933	silver sulfadiazine	915:933	silver sulfadiazine (SSD)	915:939	Middle layer was loaded with epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF), and silver sulfadiazine (SSD) was incorporated in the bottom layer as an anti-infection factor.
30311249	8	67	theme	designed	1302:1309	arg1	mat					1311:1313	designed mat	1302:1313	designed mat	1302:1313	At day 14, the treated group by designed mat showed faster epithelialization and angiogenesis.
30311249	1	68	from	topic	343:347	arg1	healing					358:364	wound healing	352:364	wound healing	352:364	Fabrication of nanofibrous biomaterials composed of natural and synthetic materials that incorporated with antibiotic and growth factors with controlled release manner is an attractive topic in wound healing.
30311249	0	69	theme	polycaprolactone	15:30	arg1	Application					0:10	Application	0:10	Application of polycaprolactone, chitosan, and collagen composite as a nanofibrous mat	0:85	Application of polycaprolactone, chitosan, and collagen composite as a nanofibrous mat loaded with silver sulfadiazine and growth factors for wound dressing.
30311249	4	70	from	compatibilities	749:763	arg1	integration					781:791	integration	781:791	integration	781:791	A panel of standard characterization tests of nanofibrous mat was performed and its compatibilities in strength and integration were confirmed.
30311249	4	70	from	compatibilities	749:763	arg1	strength					768:775	strength	768:775	strength	768:775	A panel of standard characterization tests of nanofibrous mat was performed and its compatibilities in strength and integration were confirmed.
30311249	10	71	theme	tissue	1479:1484	arg1	Reformation					1459:1469	Reformation	1459:1469	Reformation of skin tissue	1459:1484	Reformation of skin tissue was manifested in a shorter time.
30311249	3	72	from	polycaprolactone	528:543	arg1	layer					658:662	the top layer	650:662	the top layer	650:662	The mat was prepared of polycaprolactone (PCL) in the bottom, chitosan/poly ethylene oxide (Cs/PEO) in the middle, and PCL/collagen (PCL/Coll) in the top layer.
30311249	3	72	from	polycaprolactone	528:543	arg1	middle					611:616	the middle	607:616	the middle	607:616	The mat was prepared of polycaprolactone (PCL) in the bottom, chitosan/poly ethylene oxide (Cs/PEO) in the middle, and PCL/collagen (PCL/Coll) in the top layer.
30311249	3	72	from	polycaprolactone	528:543	arg1	bottom					558:563	the bottom	554:563	the bottom	554:563	The mat was prepared of polycaprolactone (PCL) in the bottom, chitosan/poly ethylene oxide (Cs/PEO) in the middle, and PCL/collagen (PCL/Coll) in the top layer.
30311249	8	73	dep	faster	1322:1327	arg1	angiogenesis					1351:1362	angiogenesis	1351:1362	angiogenesis	1351:1362	At day 14, the treated group by designed mat showed faster epithelialization and angiogenesis.
30311249	8	73	dep	faster	1322:1327	arg1	epithelialization					1329:1345	epithelialization	1329:1345	epithelialization	1329:1345	At day 14, the treated group by designed mat showed faster epithelialization and angiogenesis.
31792056	0	0	theme	facile	75:80	arg1	filtration					82:91	facile filtration	75:91	facile filtration	75:91	A capture and release method based on noncovalent ligand cross-linking and facile filtration for purification of lectins and glycoproteins.
31792056	12	1	theme	human	1522:1526	arg1	galectin-3					1528:1537	recombinant human galectin-3	1510:1537	recombinant human galectin-3	1510:1537	We validated CaRe by purifying recombinant human galectin-3 and five other known lectins and also tested CaRe's ability to purify glycoproteins.
31792056	14	2	theme	multivalent	1817:1827	arg1	ligands					1829:1835	multivalent ligands	1817:1835	multivalent ligands	1817:1835	This technique could also be used for nonlectin proteins that bind multivalent ligands.
31792056	9	3	theme	competitive	1112:1122	arg1	ligands					1135:1141	competitive monovalent ligands	1112:1141	competitive monovalent ligands	1112:1141	The lectins are captured in the solution phase by multivalent capturing agents, released by competitive monovalent ligands, and then separated by filtration.
31792056	4	4	theme	trial-and-error	633:647	arg1	experimentation					649:663	extensive trial-and-error experimentation	623:663	extensive trial-and-error experimentation	623:663	These approaches, however, are time-consuming, consist of multiple steps, and often require extensive trial-and-error experimentation.
31792056	15	5	theme	broad	1913:1917	arg1	utility					1919:1925	broad utility	1913:1925	broad utility	1913:1925	Given the ubiquity of glycosylation in nature, we anticipate that CaRe has broad utility.
31792056	9	6	theme	monovalent	1124:1133	arg1	ligands					1135:1141	competitive monovalent ligands	1112:1141	competitive monovalent ligands	1112:1141	The lectins are captured in the solution phase by multivalent capturing agents, released by competitive monovalent ligands, and then separated by filtration.
31792056	4	7	theme	extensive	623:631	arg1	experimentation					649:663	extensive trial-and-error experimentation	623:663	extensive trial-and-error experimentation	623:663	These approaches, however, are time-consuming, consist of multiple steps, and often require extensive trial-and-error experimentation.
31792056	3	8	theme	purification	391:402	arg1	chromatography					443:456	affinity column chromatography	427:456	especially affinity column chromatography	416:456	Conventional purification techniques, especially affinity column chromatography, have been instrumental in isolating numerous lectins and glycoproteins.
31792056	3	8	theme	purification	391:402	arg1	techniques					404:413	Conventional purification techniques	378:413	Conventional purification techniques	378:413	Conventional purification techniques, especially affinity column chromatography, have been instrumental in isolating numerous lectins and glycoproteins.
31792056	10	9	theme	customized	1262:1271	arg1	apparatus					1273:1281	a customized apparatus	1260:1281	a customized apparatus	1260:1281	CaRe does not require antibodies, solid affinity matrices, specialized detectors, a customized apparatus, controlled environments, or functionalization or covalent modification of reagents.
31792056	11	10	theme	extracts	1469:1476	arg1	milliliters					1440:1450	a few milliliters	1434:1450	a few milliliters of crude protein extracts	1434:1476	CaRe is a time-saving procedure that can purify lectins even from a few milliliters of crude protein extracts.
31792056	11	11	theme	time-saving	1378:1388	arg1	CaRe					1368:1371	CaRe	1368:1371	CaRe	1368:1371	CaRe is a time-saving procedure that can purify lectins even from a few milliliters of crude protein extracts.
31792056	11	11	theme	time-saving	1378:1388	arg1	procedure					1390:1398	a time-saving procedure	1376:1398	a time-saving procedure that can purify lectins even from a few milliliters of crude protein extracts	1376:1476	CaRe is a time-saving procedure that can purify lectins even from a few milliliters of crude protein extracts.
31792056	10	12	theme	covalent	1333:1340	arg1	modification					1342:1353	covalent modification	1333:1353	covalent modification	1333:1353	CaRe does not require antibodies, solid affinity matrices, specialized detectors, a customized apparatus, controlled environments, or functionalization or covalent modification of reagents.
31792056	2	13	theme	structure-function	293:310	arg1	relationships					312:324	structure-function relationships	293:324	structure-function relationships	293:324	To study their various biological activities and structure-function relationships, researchers must use lectins in their purest form.
31792056	3	14	theme	Conventional	378:389	arg1	chromatography					443:456	affinity column chromatography	427:456	especially affinity column chromatography	416:456	Conventional purification techniques, especially affinity column chromatography, have been instrumental in isolating numerous lectins and glycoproteins.
31792056	3	14	theme	Conventional	378:389	arg1	techniques					404:413	Conventional purification techniques	378:413	Conventional purification techniques	378:413	Conventional purification techniques, especially affinity column chromatography, have been instrumental in isolating numerous lectins and glycoproteins.
31792056	12	15	theme	recombinant	1510:1520	arg1	galectin-3					1528:1537	recombinant human galectin-3	1510:1537	recombinant human galectin-3	1510:1537	We validated CaRe by purifying recombinant human galectin-3 and five other known lectins and also tested CaRe's ability to purify glycoproteins.
31792056	10	16	theme	reagents	1358:1365	arg1	detectors					1249:1257	specialized detectors	1237:1257	specialized detectors	1237:1257	CaRe does not require antibodies, solid affinity matrices, specialized detectors, a customized apparatus, controlled environments, or functionalization or covalent modification of reagents.
31792056	10	16	theme	reagents	1358:1365	arg1	environments					1295:1306	controlled environments	1284:1306	controlled environments	1284:1306	CaRe does not require antibodies, solid affinity matrices, specialized detectors, a customized apparatus, controlled environments, or functionalization or covalent modification of reagents.
31792056	10	16	theme	reagents	1358:1365	arg1	matrices					1227:1234	solid affinity matrices	1212:1234	solid affinity matrices	1212:1234	CaRe does not require antibodies, solid affinity matrices, specialized detectors, a customized apparatus, controlled environments, or functionalization or covalent modification of reagents.
31792056	10	16	theme	reagents	1358:1365	arg1	apparatus					1273:1281	a customized apparatus	1260:1281	a customized apparatus	1260:1281	CaRe does not require antibodies, solid affinity matrices, specialized detectors, a customized apparatus, controlled environments, or functionalization or covalent modification of reagents.
31792056	10	16	theme	reagents	1358:1365	arg1	functionalization					1312:1328	functionalization	1312:1328	functionalization	1312:1328	CaRe does not require antibodies, solid affinity matrices, specialized detectors, a customized apparatus, controlled environments, or functionalization or covalent modification of reagents.
31792056	10	16	theme	reagents	1358:1365	arg1	antibodies					1200:1209	antibodies	1200:1209	antibodies	1200:1209	CaRe does not require antibodies, solid affinity matrices, specialized detectors, a customized apparatus, controlled environments, or functionalization or covalent modification of reagents.
31792056	10	16	theme	reagents	1358:1365	arg1	modification					1342:1353	covalent modification	1333:1353	covalent modification	1333:1353	CaRe does not require antibodies, solid affinity matrices, specialized detectors, a customized apparatus, controlled environments, or functionalization or covalent modification of reagents.
31792056	3	17	theme	numerous	495:502	arg1	lectins					504:510	lectins	504:510	lectins	504:510	Conventional purification techniques, especially affinity column chromatography, have been instrumental in isolating numerous lectins and glycoproteins.
31792056	0	18	theme	capture	2:8	arg1	method					22:27	A capture and release method	0:27	A capture and release method	0:27	A capture and release method based on noncovalent ligand cross-linking and facile filtration for purification of lectins and glycoproteins.
31792056	1	19	theme	ubiquitous	184:193	arg1	proteins					155:162	Glycan-binding proteins	140:162	Glycan-binding proteins such as lectins	140:178	Glycan-binding proteins such as lectins are ubiquitous proteins that mediate many biological functions.
31792056	1	19	theme	ubiquitous	184:193	arg1	lectins					172:178	lectins	172:178	lectins	172:178	Glycan-binding proteins such as lectins are ubiquitous proteins that mediate many biological functions.
31792056	1	19	theme	ubiquitous	184:193	arg1	proteins					195:202	ubiquitous proteins	184:202	ubiquitous proteins that mediate many biological functions	184:241	Glycan-binding proteins such as lectins are ubiquitous proteins that mediate many biological functions.
31792056	3	20	theme	column	436:441	arg1	chromatography					443:456	affinity column chromatography	427:456	especially affinity column chromatography	416:456	Conventional purification techniques, especially affinity column chromatography, have been instrumental in isolating numerous lectins and glycoproteins.
31792056	3	20	theme	column	436:441	arg1	techniques					404:413	Conventional purification techniques	378:413	Conventional purification techniques	378:413	Conventional purification techniques, especially affinity column chromatography, have been instrumental in isolating numerous lectins and glycoproteins.
31792056	8	21	theme	spontaneous	936:946	arg1	complexes					975:983	spontaneous noncovalently cross-linked complexes	936:983	spontaneous noncovalently cross-linked complexes	936:983	CaRe purifies lectins by utilizing their ability to form spontaneous noncovalently cross-linked complexes with specific multivalent ligands.
31792056	0	22	theme	release	14:20	arg1	method					22:27	A capture and release method	0:27	A capture and release method	0:27	A capture and release method based on noncovalent ligand cross-linking and facile filtration for purification of lectins and glycoproteins.
31792056	0	23	dep	capture	2:8	arg1	A					0:0	A	0:0	A	0:0	A capture and release method based on noncovalent ligand cross-linking and facile filtration for purification of lectins and glycoproteins.
31792056	14	24	used	used	1779:1782	arg2	technique					1755:1763	This technique	1750:1763	This technique	1750:1763	This technique could also be used for nonlectin proteins that bind multivalent ligands.
31792056	13	25	contain	has	1674:1676	arg2	potential					1682:1690	the potential to purify other glycoconjugates, including proteoglycans	1678:1747	the potential to purify other glycoconjugates, including proteoglycans	1678:1747	Besides purifying lectins and glycoproteins, CaRe has the potential to purify other glycoconjugates, including proteoglycans.
31792056	13	25	contain	has	1674:1676	arg1	CaRe					1669:1672	CaRe	1669:1672	CaRe	1669:1672	Besides purifying lectins and glycoproteins, CaRe has the potential to purify other glycoconjugates, including proteoglycans.
31792056	0	26	theme	lectins	113:119	arg1	purification					97:108	purification	97:108	purification of lectins and glycoproteins	97:137	A capture and release method based on noncovalent ligand cross-linking and facile filtration for purification of lectins and glycoproteins.
31792056	15	27	from	ubiquity	1848:1855	arg1	nature					1877:1882	nature	1877:1882	nature	1877:1882	Given the ubiquity of glycosylation in nature, we anticipate that CaRe has broad utility.
31792056	8	28	theme	cross-linked	962:973	arg1	complexes					975:983	spontaneous noncovalently cross-linked complexes	936:983	spontaneous noncovalently cross-linked complexes	936:983	CaRe purifies lectins by utilizing their ability to form spontaneous noncovalently cross-linked complexes with specific multivalent ligands.
31792056	9	29	theme	solution	1052:1059	arg1	phase					1061:1065	the solution phase	1048:1065	the solution phase by multivalent capturing agents	1048:1097	The lectins are captured in the solution phase by multivalent capturing agents, released by competitive monovalent ligands, and then separated by filtration.
31792056	14	30	theme	nonlectin	1788:1796	arg1	proteins					1798:1805	nonlectin proteins	1788:1805	nonlectin proteins that bind multivalent ligands	1788:1835	This technique could also be used for nonlectin proteins that bind multivalent ligands.
31792056	15	31	theme	glycosylation	1860:1872	arg1	ubiquity					1848:1855	the ubiquity	1844:1855	the ubiquity of glycosylation in nature	1844:1882	Given the ubiquity of glycosylation in nature, we anticipate that CaRe has broad utility.
31792056	1	32	theme	many	217:220	arg1	functions					233:241	many biological functions	217:241	many biological functions	217:241	Glycan-binding proteins such as lectins are ubiquitous proteins that mediate many biological functions.
31792056	11	33	theme	few	1436:1438	arg1	milliliters					1440:1450	a few milliliters	1434:1450	a few milliliters of crude protein extracts	1434:1476	CaRe is a time-saving procedure that can purify lectins even from a few milliliters of crude protein extracts.
31792056	13	34	gly	glycoproteins	1654:1666	arg1	glycoproteins					1654:1666	glycoproteins	1654:1666	glycoproteins	1654:1666	Besides purifying lectins and glycoproteins, CaRe has the potential to purify other glycoconjugates, including proteoglycans.
31792056	0	35	theme	glycoproteins	125:137	arg1	purification					97:108	purification	97:108	purification of lectins and glycoproteins	97:137	A capture and release method based on noncovalent ligand cross-linking and facile filtration for purification of lectins and glycoproteins.
31792056	3	36	gly	glycoproteins	516:528	arg1	glycoproteins					516:528	glycoproteins	516:528	glycoproteins	516:528	Conventional purification techniques, especially affinity column chromatography, have been instrumental in isolating numerous lectins and glycoproteins.
31792056	15	37	contain	has	1909:1911	arg1	CaRe					1904:1907	CaRe	1904:1907	CaRe	1904:1907	Given the ubiquity of glycosylation in nature, we anticipate that CaRe has broad utility.
31792056	15	37	contain	has	1909:1911	arg2	utility					1919:1925	broad utility	1913:1925	broad utility	1913:1925	Given the ubiquity of glycosylation in nature, we anticipate that CaRe has broad utility.
31792056	1	38	theme	biological	222:231	arg1	functions					233:241	many biological functions	217:241	many biological functions	217:241	Glycan-binding proteins such as lectins are ubiquitous proteins that mediate many biological functions.
31792056	0	39	theme	noncovalent	38:48	arg1	cross-linking					57:69	noncovalent ligand cross-linking	38:69	noncovalent ligand cross-linking	38:69	A capture and release method based on noncovalent ligand cross-linking and facile filtration for purification of lectins and glycoproteins.
31792056	7	40	theme	approach	828:835	arg1	strength					811:818	The strength	807:818	The strength of this approach	807:835	The strength of this approach is rooted in its simplicity and accuracy.
31792056	8	41	link	cross-linked	962:973	arg1	complexes					975:983	spontaneous noncovalently cross-linked complexes	936:983	spontaneous noncovalently cross-linked complexes	936:983	CaRe purifies lectins by utilizing their ability to form spontaneous noncovalently cross-linked complexes with specific multivalent ligands.
31792056	11	42	theme	protein	1461:1467	arg1	extracts					1469:1476	crude protein extracts	1455:1476	crude protein extracts	1455:1476	CaRe is a time-saving procedure that can purify lectins even from a few milliliters of crude protein extracts.
31792056	9	43	theme	multivalent	1070:1080	arg1	agents					1092:1097	multivalent capturing agents	1070:1097	multivalent capturing agents	1070:1097	The lectins are captured in the solution phase by multivalent capturing agents, released by competitive monovalent ligands, and then separated by filtration.
31792056	10	44	theme	solid	1212:1216	arg1	matrices					1227:1234	solid affinity matrices	1212:1234	solid affinity matrices	1212:1234	CaRe does not require antibodies, solid affinity matrices, specialized detectors, a customized apparatus, controlled environments, or functionalization or covalent modification of reagents.
31792056	13	45	theme	other	1702:1706	arg1	glycoconjugates					1708:1722	other glycoconjugates	1702:1722	other glycoconjugates	1702:1722	Besides purifying lectins and glycoproteins, CaRe has the potential to purify other glycoconjugates, including proteoglycans.
31792056	13	45	theme	other	1702:1706	arg1	proteoglycans					1735:1747	proteoglycans	1735:1747	proteoglycans	1735:1747	Besides purifying lectins and glycoproteins, CaRe has the potential to purify other glycoconjugates, including proteoglycans.
31792056	12	46	theme	known	1554:1558	arg1	lectins					1560:1566	five other known lectins	1543:1566	five other known lectins	1543:1566	We validated CaRe by purifying recombinant human galectin-3 and five other known lectins and also tested CaRe's ability to purify glycoproteins.
31792056	8	47	theme	multivalent	999:1009	arg1	ligands					1011:1017	specific multivalent ligands	990:1017	specific multivalent ligands	990:1017	CaRe purifies lectins by utilizing their ability to form spontaneous noncovalently cross-linked complexes with specific multivalent ligands.
31792056	2	48	theme	purest	365:370	arg1	form					372:375	their purest form	359:375	their purest form	359:375	To study their various biological activities and structure-function relationships, researchers must use lectins in their purest form.
31792056	9	49	theme	capturing	1082:1090	arg1	agents					1092:1097	multivalent capturing agents	1070:1097	multivalent capturing agents	1070:1097	The lectins are captured in the solution phase by multivalent capturing agents, released by competitive monovalent ligands, and then separated by filtration.
31792056	4	50	theme	multiple	589:596	arg1	steps					598:602	multiple steps	589:602	multiple steps	589:602	These approaches, however, are time-consuming, consist of multiple steps, and often require extensive trial-and-error experimentation.
31792056	12	51	theme	other	1548:1552	arg1	lectins					1560:1566	five other known lectins	1543:1566	five other known lectins	1543:1566	We validated CaRe by purifying recombinant human galectin-3 and five other known lectins and also tested CaRe's ability to purify glycoproteins.
31792056	8	52	theme	specific	990:997	arg1	ligands					1011:1017	specific multivalent ligands	990:1017	specific multivalent ligands	990:1017	CaRe purifies lectins by utilizing their ability to form spontaneous noncovalently cross-linked complexes with specific multivalent ligands.
31792056	0	53	gly	glycoproteins	125:137	arg1	glycoproteins					125:137	glycoproteins	125:137	glycoproteins	125:137	A capture and release method based on noncovalent ligand cross-linking and facile filtration for purification of lectins and glycoproteins.
31792056	2	54	theme	biological	267:276	arg1	activities					278:287	their various biological activities	253:287	their various biological activities	253:287	To study their various biological activities and structure-function relationships, researchers must use lectins in their purest form.
31792056	11	55	theme	crude	1455:1459	arg1	extracts					1469:1476	crude protein extracts	1455:1476	crude protein extracts	1455:1476	CaRe is a time-saving procedure that can purify lectins even from a few milliliters of crude protein extracts.
31792056	2	56	theme	various	259:265	arg1	activities					278:287	their various biological activities	253:287	their various biological activities	253:287	To study their various biological activities and structure-function relationships, researchers must use lectins in their purest form.
31792056	1	57	theme	Glycan-binding	140:153	arg1	proteins					155:162	Glycan-binding proteins	140:162	Glycan-binding proteins such as lectins	140:178	Glycan-binding proteins such as lectins are ubiquitous proteins that mediate many biological functions.
31792056	1	57	theme	Glycan-binding	140:153	arg1	lectins					172:178	lectins	172:178	lectins	172:178	Glycan-binding proteins such as lectins are ubiquitous proteins that mediate many biological functions.
31792056	1	57	theme	Glycan-binding	140:153	arg1	proteins					195:202	ubiquitous proteins	184:202	ubiquitous proteins that mediate many biological functions	184:241	Glycan-binding proteins such as lectins are ubiquitous proteins that mediate many biological functions.
31792056	10	58	theme	controlled	1284:1293	arg1	environments					1295:1306	controlled environments	1284:1306	controlled environments	1284:1306	CaRe does not require antibodies, solid affinity matrices, specialized detectors, a customized apparatus, controlled environments, or functionalization or covalent modification of reagents.
31792056	10	59	theme	specialized	1237:1247	arg1	detectors					1249:1257	specialized detectors	1237:1257	specialized detectors	1237:1257	CaRe does not require antibodies, solid affinity matrices, specialized detectors, a customized apparatus, controlled environments, or functionalization or covalent modification of reagents.
31792056	0	60	theme	ligand	50:55	arg1	cross-linking					57:69	noncovalent ligand cross-linking	38:69	noncovalent ligand cross-linking	38:69	A capture and release method based on noncovalent ligand cross-linking and facile filtration for purification of lectins and glycoproteins.
31792056	12	61	gly	glycoproteins	1609:1621	arg1	glycoproteins					1609:1621	glycoproteins	1609:1621	glycoproteins	1609:1621	We validated CaRe by purifying recombinant human galectin-3 and five other known lectins and also tested CaRe's ability to purify glycoproteins.
31792056	3	62	theme	affinity	427:434	arg1	chromatography					443:456	affinity column chromatography	427:456	especially affinity column chromatography	416:456	Conventional purification techniques, especially affinity column chromatography, have been instrumental in isolating numerous lectins and glycoproteins.
31792056	3	62	theme	affinity	427:434	arg1	techniques					404:413	Conventional purification techniques	378:413	Conventional purification techniques	378:413	Conventional purification techniques, especially affinity column chromatography, have been instrumental in isolating numerous lectins and glycoproteins.
31792056	10	63	theme	affinity	1218:1225	arg1	matrices					1227:1234	solid affinity matrices	1212:1234	solid affinity matrices	1212:1234	CaRe does not require antibodies, solid affinity matrices, specialized detectors, a customized apparatus, controlled environments, or functionalization or covalent modification of reagents.
31558017	8	0	theme	hydrogel	1639:1646	arg1	stability					1612:1620	the stability	1608:1620	the stability of the composite hydrogel	1608:1646	Furthermore, the formulation could also exert strong photoacoustic signals, which were utilized to monitor the stability of the composite hydrogel.
31558017	2	1	theme	cells	338:342	arg1	resistance					318:327	that inducible heat resistance	298:327	that inducible heat resistance of tumor cells	298:342	However, beyond that inducible heat resistance of tumor cells, the discounted therapeutic effect of low temperature hyperthermia was also ascribed to poor penetration of exogenous light stimulation and low accumulation of photothermal agents in tumor sites.
31558017	1	2	theme	mild	249:252	arg1	toxicity					254:261	mild toxicity	249:261	mild toxicity	249:261	As a novel treatment modality of tumors, hypothermal hyperthermia employed relatively lower temperature (<45 °C) to damage cancer cells with mild toxicity to normal tissues.
31558017	7	3	theme	side	1487:1490	arg1	effects					1492:1498	negligible side effects	1476:1498	negligible side effects	1476:1498	Thus, remarkable inhibition of tumor growth was observed in a subcutaneous colorectal cancer with negligible side effects.
31558017	3	4	theme	azo	702:704	arg1	initiator					706:714	azo initiator	702:714	azo initiator	702:714	Herein, we constructed a multifunctional in situ hydrogel of sodium alginate (ALG) via Ca2+ coordinated with ALG to encapsulate the photothermal agent of Ink and azo initiator of 2,2'-azobis[2-(2-imidazolin-2-yl)propane]dihydrochloride (AIPH) for effective tumor treatment.
31558017	5	5	theme	alkyl	1194:1198	arg1	radicals					1200:1207	alkyl radicals	1194:1207	alkyl radicals	1194:1207	After injection, local low temperature stimulation was generated with near-infrared-II irradiation by a 1064 nm laser, triggering rapid decomposition of AIPH to produce alkyl radicals.
31558017	3	6	theme	Ink	694:696	arg1	agent					685:689	the photothermal agent	668:689	the photothermal agent of Ink and azo initiator of 2,2'-azobis[2-(2-imidazolin-2-yl)propane]dihydrochloride (AIPH) for effective tumor treatment	668:811	Herein, we constructed a multifunctional in situ hydrogel of sodium alginate (ALG) via Ca2+ coordinated with ALG to encapsulate the photothermal agent of Ink and azo initiator of 2,2'-azobis[2-(2-imidazolin-2-yl)propane]dihydrochloride (AIPH) for effective tumor treatment.
31558017	6	7	theme	photothermal	1242:1253	arg1	therapy					1255:1261	photothermal therapy	1242:1261	photothermal therapy	1242:1261	The synergistic low temperature photothermal therapy and cytotoxic-free radicals enhanced the apoptosis of tumor cells via physical heat damage and lipid peroxidation.
31558017	7	8	theme	colorectal	1453:1462	arg1	cancer					1464:1469	a subcutaneous colorectal cancer	1438:1469	a subcutaneous colorectal cancer with negligible side effects	1438:1498	Thus, remarkable inhibition of tumor growth was observed in a subcutaneous colorectal cancer with negligible side effects.
31558017	4	9	theme	ALG	827:829	arg1	hydrogel					831:838	The designed ALG hydrogel	814:838	The designed ALG hydrogel	814:838	The designed ALG hydrogel was used to improve the therapeutic effect by increased accumulation of Ink and AIPH and avoid potential side-effects caused by the unexpected spread to the surrounding normal tissues.
31558017	4	10	used	used	844:847	arg2	hydrogel					831:838	The designed ALG hydrogel	814:838	The designed ALG hydrogel	814:838	The designed ALG hydrogel was used to improve the therapeutic effect by increased accumulation of Ink and AIPH and avoid potential side-effects caused by the unexpected spread to the surrounding normal tissues.
31558017	7	11	theme	tumor	1409:1413	arg1	growth					1415:1420	tumor growth	1409:1420	tumor growth	1409:1420	Thus, remarkable inhibition of tumor growth was observed in a subcutaneous colorectal cancer with negligible side effects.
31558017	3	12	theme	[2-	730:732	arg1	AIPH					777:780	AIPH	777:780	AIPH	777:780	Herein, we constructed a multifunctional in situ hydrogel of sodium alginate (ALG) via Ca2+ coordinated with ALG to encapsulate the photothermal agent of Ink and azo initiator of 2,2'-azobis[2-(2-imidazolin-2-yl)propane]dihydrochloride (AIPH) for effective tumor treatment.
31558017	3	12	theme	[2-	730:732	arg1	dihydrochloride					760:774	2,2'-azobis[2-(2-imidazolin-2-yl)propane]dihydrochloride	719:774	2,2'-azobis[2-(2-imidazolin-2-yl)propane]dihydrochloride (AIPH)	719:781	Herein, we constructed a multifunctional in situ hydrogel of sodium alginate (ALG) via Ca2+ coordinated with ALG to encapsulate the photothermal agent of Ink and azo initiator of 2,2'-azobis[2-(2-imidazolin-2-yl)propane]dihydrochloride (AIPH) for effective tumor treatment.
31558017	6	13	theme	low	1226:1228	arg1	temperature					1230:1240	The synergistic low temperature photothermal therapy and cytotoxic-free radicals	1210:1289	temperature	1230:1240	The synergistic low temperature photothermal therapy and cytotoxic-free radicals enhanced the apoptosis of tumor cells via physical heat damage and lipid peroxidation.
31558017	3	14	theme	multifunctional	565:579	arg1	hydrogel					589:596	a multifunctional in situ hydrogel	563:596	a multifunctional in situ hydrogel of sodium alginate (ALG)	563:621	Herein, we constructed a multifunctional in situ hydrogel of sodium alginate (ALG) via Ca2+ coordinated with ALG to encapsulate the photothermal agent of Ink and azo initiator of 2,2'-azobis[2-(2-imidazolin-2-yl)propane]dihydrochloride (AIPH) for effective tumor treatment.
31558017	4	15	theme	increased	886:894	arg1	accumulation					896:907	increased accumulation	886:907	increased accumulation of Ink and AIPH	886:923	The designed ALG hydrogel was used to improve the therapeutic effect by increased accumulation of Ink and AIPH and avoid potential side-effects caused by the unexpected spread to the surrounding normal tissues.
31558017	6	16	theme	physical	1333:1340	arg1	damage					1347:1352	physical heat damage	1333:1352	physical heat damage	1333:1352	The synergistic low temperature photothermal therapy and cytotoxic-free radicals enhanced the apoptosis of tumor cells via physical heat damage and lipid peroxidation.
31558017	6	17	theme	synergistic	1214:1224	arg1	temperature					1230:1240	The synergistic low temperature photothermal therapy and cytotoxic-free radicals	1210:1289	temperature	1230:1240	The synergistic low temperature photothermal therapy and cytotoxic-free radicals enhanced the apoptosis of tumor cells via physical heat damage and lipid peroxidation.
31558017	3	18	theme	propane	752:758	arg1	AIPH					777:780	AIPH	777:780	AIPH	777:780	Herein, we constructed a multifunctional in situ hydrogel of sodium alginate (ALG) via Ca2+ coordinated with ALG to encapsulate the photothermal agent of Ink and azo initiator of 2,2'-azobis[2-(2-imidazolin-2-yl)propane]dihydrochloride (AIPH) for effective tumor treatment.
31558017	3	18	theme	propane	752:758	arg1	dihydrochloride					760:774	2,2'-azobis[2-(2-imidazolin-2-yl)propane]dihydrochloride	719:774	2,2'-azobis[2-(2-imidazolin-2-yl)propane]dihydrochloride (AIPH)	719:781	Herein, we constructed a multifunctional in situ hydrogel of sodium alginate (ALG) via Ca2+ coordinated with ALG to encapsulate the photothermal agent of Ink and azo initiator of 2,2'-azobis[2-(2-imidazolin-2-yl)propane]dihydrochloride (AIPH) for effective tumor treatment.
31558017	6	19	theme	cells	1323:1327	arg1	apoptosis					1304:1312	the apoptosis	1300:1312	the apoptosis of tumor cells	1300:1327	The synergistic low temperature photothermal therapy and cytotoxic-free radicals enhanced the apoptosis of tumor cells via physical heat damage and lipid peroxidation.
31558017	5	20	theme	low	1048:1050	arg1	temperature					1052:1062	local low temperature	1042:1062	local low temperature stimulation	1042:1074	After injection, local low temperature stimulation was generated with near-infrared-II irradiation by a 1064 nm laser, triggering rapid decomposition of AIPH to produce alkyl radicals.
31558017	2	21	from	accumulation	488:499	arg1	sites					533:537	tumor sites	527:537	tumor sites	527:537	However, beyond that inducible heat resistance of tumor cells, the discounted therapeutic effect of low temperature hyperthermia was also ascribed to poor penetration of exogenous light stimulation and low accumulation of photothermal agents in tumor sites.
31558017	3	22	theme	2-imidazolin-2-yl	734:750	arg1	AIPH					777:780	AIPH	777:780	AIPH	777:780	Herein, we constructed a multifunctional in situ hydrogel of sodium alginate (ALG) via Ca2+ coordinated with ALG to encapsulate the photothermal agent of Ink and azo initiator of 2,2'-azobis[2-(2-imidazolin-2-yl)propane]dihydrochloride (AIPH) for effective tumor treatment.
31558017	3	22	theme	2-imidazolin-2-yl	734:750	arg1	dihydrochloride					760:774	2,2'-azobis[2-(2-imidazolin-2-yl)propane]dihydrochloride	719:774	2,2'-azobis[2-(2-imidazolin-2-yl)propane]dihydrochloride (AIPH)	719:781	Herein, we constructed a multifunctional in situ hydrogel of sodium alginate (ALG) via Ca2+ coordinated with ALG to encapsulate the photothermal agent of Ink and azo initiator of 2,2'-azobis[2-(2-imidazolin-2-yl)propane]dihydrochloride (AIPH) for effective tumor treatment.
31558017	1	23	theme	treatment	119:127	arg1	modality					129:136	a novel treatment modality	111:136	a novel treatment modality of tumors	111:146	As a novel treatment modality of tumors, hypothermal hyperthermia employed relatively lower temperature (<45 °C) to damage cancer cells with mild toxicity to normal tissues.
31558017	1	23	theme	treatment	119:127	arg1	temperature					200:210	relatively lower temperature	183:210	relatively lower temperature (<45 °C) to damage cancer cells with mild toxicity to normal tissues	183:279	As a novel treatment modality of tumors, hypothermal hyperthermia employed relatively lower temperature (<45 °C) to damage cancer cells with mild toxicity to normal tissues.
31558017	4	24	theme	potential	935:943	arg1	side-effects					945:956	potential side-effects	935:956	potential side-effects caused by the unexpected spread to the surrounding normal tissues	935:1022	The designed ALG hydrogel was used to improve the therapeutic effect by increased accumulation of Ink and AIPH and avoid potential side-effects caused by the unexpected spread to the surrounding normal tissues.
31558017	3	25	theme	dihydrochloride	760:774	arg1	Ink					694:696	Ink	694:696	Ink	694:696	Herein, we constructed a multifunctional in situ hydrogel of sodium alginate (ALG) via Ca2+ coordinated with ALG to encapsulate the photothermal agent of Ink and azo initiator of 2,2'-azobis[2-(2-imidazolin-2-yl)propane]dihydrochloride (AIPH) for effective tumor treatment.
31558017	3	25	theme	dihydrochloride	760:774	arg1	initiator					706:714	azo initiator	702:714	azo initiator	702:714	Herein, we constructed a multifunctional in situ hydrogel of sodium alginate (ALG) via Ca2+ coordinated with ALG to encapsulate the photothermal agent of Ink and azo initiator of 2,2'-azobis[2-(2-imidazolin-2-yl)propane]dihydrochloride (AIPH) for effective tumor treatment.
31558017	5	26	theme	near-infrared-II	1095:1110	arg1	irradiation					1112:1122	near-infrared-II irradiation	1095:1122	near-infrared-II irradiation	1095:1122	After injection, local low temperature stimulation was generated with near-infrared-II irradiation by a 1064 nm laser, triggering rapid decomposition of AIPH to produce alkyl radicals.
31558017	3	27	theme	alginate	608:615	arg1	hydrogel					589:596	a multifunctional in situ hydrogel	563:596	a multifunctional in situ hydrogel of sodium alginate (ALG)	563:621	Herein, we constructed a multifunctional in situ hydrogel of sodium alginate (ALG) via Ca2+ coordinated with ALG to encapsulate the photothermal agent of Ink and azo initiator of 2,2'-azobis[2-(2-imidazolin-2-yl)propane]dihydrochloride (AIPH) for effective tumor treatment.
31558017	2	28	theme	low	484:486	arg1	accumulation					488:499	low accumulation	484:499	low accumulation of photothermal agents in tumor sites	484:537	However, beyond that inducible heat resistance of tumor cells, the discounted therapeutic effect of low temperature hyperthermia was also ascribed to poor penetration of exogenous light stimulation and low accumulation of photothermal agents in tumor sites.
31558017	3	29	theme	tumor	797:801	arg1	treatment					803:811	effective tumor treatment	787:811	effective tumor treatment	787:811	Herein, we constructed a multifunctional in situ hydrogel of sodium alginate (ALG) via Ca2+ coordinated with ALG to encapsulate the photothermal agent of Ink and azo initiator of 2,2'-azobis[2-(2-imidazolin-2-yl)propane]dihydrochloride (AIPH) for effective tumor treatment.
31558017	0	30	theme	Low-Temperature	70:84	arg1	Therapy					99:105	Augmented Low-Temperature Photothermal Therapy	60:105	Augmented Low-Temperature Photothermal Therapy	60:105	Localized Free Radicals Burst Triggered by NIR-II Light for Augmented Low-Temperature Photothermal Therapy.
31558017	2	31	theme	tumor	527:531	arg1	sites					533:537	tumor sites	527:537	tumor sites	527:537	However, beyond that inducible heat resistance of tumor cells, the discounted therapeutic effect of low temperature hyperthermia was also ascribed to poor penetration of exogenous light stimulation and low accumulation of photothermal agents in tumor sites.
31558017	6	32	theme	lipid	1358:1362	arg1	peroxidation					1364:1375	lipid peroxidation	1358:1375	lipid peroxidation	1358:1375	The synergistic low temperature photothermal therapy and cytotoxic-free radicals enhanced the apoptosis of tumor cells via physical heat damage and lipid peroxidation.
31558017	2	33	theme	agents	517:522	arg1	accumulation					488:499	low accumulation	484:499	low accumulation of photothermal agents in tumor sites	484:537	However, beyond that inducible heat resistance of tumor cells, the discounted therapeutic effect of low temperature hyperthermia was also ascribed to poor penetration of exogenous light stimulation and low accumulation of photothermal agents in tumor sites.
31558017	2	33	theme	agents	517:522	arg1	penetration					437:447	poor penetration	432:447	poor penetration of exogenous light stimulation	432:478	However, beyond that inducible heat resistance of tumor cells, the discounted therapeutic effect of low temperature hyperthermia was also ascribed to poor penetration of exogenous light stimulation and low accumulation of photothermal agents in tumor sites.
31558017	2	34	theme	low	382:384	arg1	hyperthermia					398:409	low temperature hyperthermia	382:409	low temperature hyperthermia	382:409	However, beyond that inducible heat resistance of tumor cells, the discounted therapeutic effect of low temperature hyperthermia was also ascribed to poor penetration of exogenous light stimulation and low accumulation of photothermal agents in tumor sites.
31558017	0	35	theme	Localized	0:8	arg1	Radicals					15:22	Localized Free Radicals	0:22	Localized Free Radicals Burst	0:28	Localized Free Radicals Burst Triggered by NIR-II Light for Augmented Low-Temperature Photothermal Therapy.
31558017	1	36	theme	normal	266:271	arg1	tissues					273:279	normal tissues	266:279	normal tissues	266:279	As a novel treatment modality of tumors, hypothermal hyperthermia employed relatively lower temperature (<45 °C) to damage cancer cells with mild toxicity to normal tissues.
31558017	4	37	theme	normal	1009:1014	arg1	tissues					1016:1022	the surrounding normal tissues	993:1022	the surrounding normal tissues	993:1022	The designed ALG hydrogel was used to improve the therapeutic effect by increased accumulation of Ink and AIPH and avoid potential side-effects caused by the unexpected spread to the surrounding normal tissues.
31558017	8	38	theme	photoacoustic	1554:1566	arg1	signals					1568:1574	strong photoacoustic signals	1547:1574	strong photoacoustic signals	1547:1574	Furthermore, the formulation could also exert strong photoacoustic signals, which were utilized to monitor the stability of the composite hydrogel.
31558017	0	39	theme	Radicals	15:22	arg1	Burst					24:28	Localized Free Radicals Burst	0:28	Localized Free Radicals Burst	0:28	Localized Free Radicals Burst Triggered by NIR-II Light for Augmented Low-Temperature Photothermal Therapy.
31558017	2	40	theme	stimulation	468:478	arg1	accumulation					488:499	low accumulation	484:499	low accumulation of photothermal agents in tumor sites	484:537	However, beyond that inducible heat resistance of tumor cells, the discounted therapeutic effect of low temperature hyperthermia was also ascribed to poor penetration of exogenous light stimulation and low accumulation of photothermal agents in tumor sites.
31558017	2	40	theme	stimulation	468:478	arg1	penetration					437:447	poor penetration	432:447	poor penetration of exogenous light stimulation	432:478	However, beyond that inducible heat resistance of tumor cells, the discounted therapeutic effect of low temperature hyperthermia was also ascribed to poor penetration of exogenous light stimulation and low accumulation of photothermal agents in tumor sites.
31558017	2	41	theme	hyperthermia	398:409	arg1	effect					372:377	the discounted therapeutic effect	345:377	the discounted therapeutic effect of low temperature hyperthermia	345:409	However, beyond that inducible heat resistance of tumor cells, the discounted therapeutic effect of low temperature hyperthermia was also ascribed to poor penetration of exogenous light stimulation and low accumulation of photothermal agents in tumor sites.
31558017	5	42	theme	1064	1129:1132	arg1	nm					1134:1135	nm	1134:1135	nm	1134:1135	After injection, local low temperature stimulation was generated with near-infrared-II irradiation by a 1064 nm laser, triggering rapid decomposition of AIPH to produce alkyl radicals.
31558017	2	43	theme	exogenous	452:460	arg1	stimulation					468:478	exogenous light stimulation	452:478	exogenous light stimulation	452:478	However, beyond that inducible heat resistance of tumor cells, the discounted therapeutic effect of low temperature hyperthermia was also ascribed to poor penetration of exogenous light stimulation and low accumulation of photothermal agents in tumor sites.
31558017	2	44	theme	tumor	332:336	arg1	cells					338:342	tumor cells	332:342	tumor cells	332:342	However, beyond that inducible heat resistance of tumor cells, the discounted therapeutic effect of low temperature hyperthermia was also ascribed to poor penetration of exogenous light stimulation and low accumulation of photothermal agents in tumor sites.
31558017	0	45	theme	NIR-II	43:48	arg1	Light					50:54	NIR-II Light	43:54	NIR-II Light for Augmented Low-Temperature Photothermal Therapy	43:105	Localized Free Radicals Burst Triggered by NIR-II Light for Augmented Low-Temperature Photothermal Therapy.
31558017	7	46	located	observed	1426:1433	arg1	cancer					1464:1469	a subcutaneous colorectal cancer	1438:1469	a subcutaneous colorectal cancer with negligible side effects	1438:1498	Thus, remarkable inhibition of tumor growth was observed in a subcutaneous colorectal cancer with negligible side effects.
31558017	7	46	located	observed	1426:1433	arg2	inhibition					1395:1404	remarkable inhibition	1384:1404	remarkable inhibition of tumor growth	1384:1420	Thus, remarkable inhibition of tumor growth was observed in a subcutaneous colorectal cancer with negligible side effects.
31558017	1	47	with	temperature	200:210	arg1	toxicity					254:261	mild toxicity	249:261	mild toxicity	249:261	As a novel treatment modality of tumors, hypothermal hyperthermia employed relatively lower temperature (<45 °C) to damage cancer cells with mild toxicity to normal tissues.
31558017	8	48	used	utilized	1588:1595	arg2	signals					1568:1574	strong photoacoustic signals	1547:1574	strong photoacoustic signals	1547:1574	Furthermore, the formulation could also exert strong photoacoustic signals, which were utilized to monitor the stability of the composite hydrogel.
31558017	2	49	theme	discounted	349:358	arg1	effect					372:377	the discounted therapeutic effect	345:377	the discounted therapeutic effect of low temperature hyperthermia	345:409	However, beyond that inducible heat resistance of tumor cells, the discounted therapeutic effect of low temperature hyperthermia was also ascribed to poor penetration of exogenous light stimulation and low accumulation of photothermal agents in tumor sites.
31558017	1	50	theme	cancer	231:236	arg1	cells					238:242	damage cancer cells	224:242	damage cancer cells	224:242	As a novel treatment modality of tumors, hypothermal hyperthermia employed relatively lower temperature (<45 °C) to damage cancer cells with mild toxicity to normal tissues.
31558017	5	51	theme	AIPH	1178:1181	arg1	decomposition					1161:1173	rapid decomposition	1155:1173	rapid decomposition of AIPH to produce alkyl radicals	1155:1207	After injection, local low temperature stimulation was generated with near-infrared-II irradiation by a 1064 nm laser, triggering rapid decomposition of AIPH to produce alkyl radicals.
31558017	7	52	theme	negligible	1476:1485	arg1	effects					1492:1498	negligible side effects	1476:1498	negligible side effects	1476:1498	Thus, remarkable inhibition of tumor growth was observed in a subcutaneous colorectal cancer with negligible side effects.
31558017	8	53	theme	composite	1629:1637	arg1	hydrogel					1639:1646	the composite hydrogel	1625:1646	the composite hydrogel	1625:1646	Furthermore, the formulation could also exert strong photoacoustic signals, which were utilized to monitor the stability of the composite hydrogel.
31558017	3	54	theme	photothermal	672:683	arg1	agent					685:689	the photothermal agent	668:689	the photothermal agent of Ink and azo initiator of 2,2'-azobis[2-(2-imidazolin-2-yl)propane]dihydrochloride (AIPH) for effective tumor treatment	668:811	Herein, we constructed a multifunctional in situ hydrogel of sodium alginate (ALG) via Ca2+ coordinated with ALG to encapsulate the photothermal agent of Ink and azo initiator of 2,2'-azobis[2-(2-imidazolin-2-yl)propane]dihydrochloride (AIPH) for effective tumor treatment.
31558017	6	55	theme	cytotoxic-free	1267:1280	arg1	radicals					1282:1289	cytotoxic-free radicals	1267:1289	cytotoxic-free radicals	1267:1289	The synergistic low temperature photothermal therapy and cytotoxic-free radicals enhanced the apoptosis of tumor cells via physical heat damage and lipid peroxidation.
31558017	3	56	theme	initiator	706:714	arg1	agent					685:689	the photothermal agent	668:689	the photothermal agent of Ink and azo initiator of 2,2'-azobis[2-(2-imidazolin-2-yl)propane]dihydrochloride (AIPH) for effective tumor treatment	668:811	Herein, we constructed a multifunctional in situ hydrogel of sodium alginate (ALG) via Ca2+ coordinated with ALG to encapsulate the photothermal agent of Ink and azo initiator of 2,2'-azobis[2-(2-imidazolin-2-yl)propane]dihydrochloride (AIPH) for effective tumor treatment.
31558017	3	57	dep	in	581:582	arg1	situ					584:587	situ	584:587	situ	584:587	Herein, we constructed a multifunctional in situ hydrogel of sodium alginate (ALG) via Ca2+ coordinated with ALG to encapsulate the photothermal agent of Ink and azo initiator of 2,2'-azobis[2-(2-imidazolin-2-yl)propane]dihydrochloride (AIPH) for effective tumor treatment.
31558017	7	58	theme	subcutaneous	1440:1451	arg1	cancer					1464:1469	a subcutaneous colorectal cancer	1438:1469	a subcutaneous colorectal cancer with negligible side effects	1438:1498	Thus, remarkable inhibition of tumor growth was observed in a subcutaneous colorectal cancer with negligible side effects.
31558017	2	59	theme	inducible	303:311	arg1	resistance					318:327	that inducible heat resistance	298:327	that inducible heat resistance of tumor cells	298:342	However, beyond that inducible heat resistance of tumor cells, the discounted therapeutic effect of low temperature hyperthermia was also ascribed to poor penetration of exogenous light stimulation and low accumulation of photothermal agents in tumor sites.
31558017	1	60	theme	lower	194:198	arg1	modality					129:136	a novel treatment modality	111:136	a novel treatment modality of tumors	111:146	As a novel treatment modality of tumors, hypothermal hyperthermia employed relatively lower temperature (<45 °C) to damage cancer cells with mild toxicity to normal tissues.
31558017	1	60	theme	lower	194:198	arg1	temperature					200:210	relatively lower temperature	183:210	relatively lower temperature (<45 °C) to damage cancer cells with mild toxicity to normal tissues	183:279	As a novel treatment modality of tumors, hypothermal hyperthermia employed relatively lower temperature (<45 °C) to damage cancer cells with mild toxicity to normal tissues.
31558017	1	60	theme	lower	194:198	arg1	°C					217:218	<45 °C	213:218	<45 °C	213:218	As a novel treatment modality of tumors, hypothermal hyperthermia employed relatively lower temperature (<45 °C) to damage cancer cells with mild toxicity to normal tissues.
31558017	4	61	theme	designed	818:825	arg1	hydrogel					831:838	The designed ALG hydrogel	814:838	The designed ALG hydrogel	814:838	The designed ALG hydrogel was used to improve the therapeutic effect by increased accumulation of Ink and AIPH and avoid potential side-effects caused by the unexpected spread to the surrounding normal tissues.
31558017	7	62	theme	growth	1415:1420	arg1	inhibition					1395:1404	remarkable inhibition	1384:1404	remarkable inhibition of tumor growth	1384:1420	Thus, remarkable inhibition of tumor growth was observed in a subcutaneous colorectal cancer with negligible side effects.
31558017	6	63	theme	heat	1342:1345	arg1	damage					1347:1352	physical heat damage	1333:1352	physical heat damage	1333:1352	The synergistic low temperature photothermal therapy and cytotoxic-free radicals enhanced the apoptosis of tumor cells via physical heat damage and lipid peroxidation.
31558017	3	64	theme	2,2'-azobis	719:729	arg1	AIPH					777:780	AIPH	777:780	AIPH	777:780	Herein, we constructed a multifunctional in situ hydrogel of sodium alginate (ALG) via Ca2+ coordinated with ALG to encapsulate the photothermal agent of Ink and azo initiator of 2,2'-azobis[2-(2-imidazolin-2-yl)propane]dihydrochloride (AIPH) for effective tumor treatment.
31558017	3	64	theme	2,2'-azobis	719:729	arg1	dihydrochloride					760:774	2,2'-azobis[2-(2-imidazolin-2-yl)propane]dihydrochloride	719:774	2,2'-azobis[2-(2-imidazolin-2-yl)propane]dihydrochloride (AIPH)	719:781	Herein, we constructed a multifunctional in situ hydrogel of sodium alginate (ALG) via Ca2+ coordinated with ALG to encapsulate the photothermal agent of Ink and azo initiator of 2,2'-azobis[2-(2-imidazolin-2-yl)propane]dihydrochloride (AIPH) for effective tumor treatment.
31558017	5	65	theme	local	1042:1046	arg1	temperature					1052:1062	local low temperature	1042:1062	local low temperature stimulation	1042:1074	After injection, local low temperature stimulation was generated with near-infrared-II irradiation by a 1064 nm laser, triggering rapid decomposition of AIPH to produce alkyl radicals.
31558017	7	66	theme	remarkable	1384:1393	arg1	inhibition					1395:1404	remarkable inhibition	1384:1404	remarkable inhibition of tumor growth	1384:1420	Thus, remarkable inhibition of tumor growth was observed in a subcutaneous colorectal cancer with negligible side effects.
31558017	6	67	theme	tumor	1317:1321	arg1	cells					1323:1327	tumor cells	1317:1327	tumor cells	1317:1327	The synergistic low temperature photothermal therapy and cytotoxic-free radicals enhanced the apoptosis of tumor cells via physical heat damage and lipid peroxidation.
31558017	5	68	theme	temperature	1052:1062	arg1	stimulation					1064:1074	local low temperature stimulation	1042:1074	local low temperature stimulation	1042:1074	After injection, local low temperature stimulation was generated with near-infrared-II irradiation by a 1064 nm laser, triggering rapid decomposition of AIPH to produce alkyl radicals.
31558017	3	69	theme	in	581:582	arg1	hydrogel					589:596	a multifunctional in situ hydrogel	563:596	a multifunctional in situ hydrogel of sodium alginate (ALG)	563:621	Herein, we constructed a multifunctional in situ hydrogel of sodium alginate (ALG) via Ca2+ coordinated with ALG to encapsulate the photothermal agent of Ink and azo initiator of 2,2'-azobis[2-(2-imidazolin-2-yl)propane]dihydrochloride (AIPH) for effective tumor treatment.
31558017	1	70	theme	novel	113:117	arg1	modality					129:136	a novel treatment modality	111:136	a novel treatment modality of tumors	111:146	As a novel treatment modality of tumors, hypothermal hyperthermia employed relatively lower temperature (<45 °C) to damage cancer cells with mild toxicity to normal tissues.
31558017	1	70	theme	novel	113:117	arg1	temperature					200:210	relatively lower temperature	183:210	relatively lower temperature (<45 °C) to damage cancer cells with mild toxicity to normal tissues	183:279	As a novel treatment modality of tumors, hypothermal hyperthermia employed relatively lower temperature (<45 °C) to damage cancer cells with mild toxicity to normal tissues.
31558017	4	71	theme	therapeutic	864:874	arg1	effect					876:881	the therapeutic effect	860:881	the therapeutic effect	860:881	The designed ALG hydrogel was used to improve the therapeutic effect by increased accumulation of Ink and AIPH and avoid potential side-effects caused by the unexpected spread to the surrounding normal tissues.
31558017	0	72	theme	Augmented	60:68	arg1	Therapy					99:105	Augmented Low-Temperature Photothermal Therapy	60:105	Augmented Low-Temperature Photothermal Therapy	60:105	Localized Free Radicals Burst Triggered by NIR-II Light for Augmented Low-Temperature Photothermal Therapy.
31558017	3	73	theme	sodium	601:606	arg1	alginate					608:615	sodium alginate	601:615	sodium alginate (ALG)	601:621	Herein, we constructed a multifunctional in situ hydrogel of sodium alginate (ALG) via Ca2+ coordinated with ALG to encapsulate the photothermal agent of Ink and azo initiator of 2,2'-azobis[2-(2-imidazolin-2-yl)propane]dihydrochloride (AIPH) for effective tumor treatment.
31558017	3	73	theme	sodium	601:606	arg1	ALG					618:620	ALG	618:620	ALG	618:620	Herein, we constructed a multifunctional in situ hydrogel of sodium alginate (ALG) via Ca2+ coordinated with ALG to encapsulate the photothermal agent of Ink and azo initiator of 2,2'-azobis[2-(2-imidazolin-2-yl)propane]dihydrochloride (AIPH) for effective tumor treatment.
31558017	1	74	theme	tumors	141:146	arg1	modality					129:136	a novel treatment modality	111:136	a novel treatment modality of tumors	111:146	As a novel treatment modality of tumors, hypothermal hyperthermia employed relatively lower temperature (<45 °C) to damage cancer cells with mild toxicity to normal tissues.
31558017	1	74	theme	tumors	141:146	arg1	temperature					200:210	relatively lower temperature	183:210	relatively lower temperature (<45 °C) to damage cancer cells with mild toxicity to normal tissues	183:279	As a novel treatment modality of tumors, hypothermal hyperthermia employed relatively lower temperature (<45 °C) to damage cancer cells with mild toxicity to normal tissues.
31558017	4	75	theme	AIPH	920:923	arg1	accumulation					896:907	increased accumulation	886:907	increased accumulation of Ink and AIPH	886:923	The designed ALG hydrogel was used to improve the therapeutic effect by increased accumulation of Ink and AIPH and avoid potential side-effects caused by the unexpected spread to the surrounding normal tissues.
31558017	0	76	theme	Photothermal	86:97	arg1	Therapy					99:105	Augmented Low-Temperature Photothermal Therapy	60:105	Augmented Low-Temperature Photothermal Therapy	60:105	Localized Free Radicals Burst Triggered by NIR-II Light for Augmented Low-Temperature Photothermal Therapy.
31558017	1	77	theme	hypothermal	149:159	arg1	hyperthermia					161:172	hypothermal hyperthermia	149:172	hypothermal hyperthermia	149:172	As a novel treatment modality of tumors, hypothermal hyperthermia employed relatively lower temperature (<45 °C) to damage cancer cells with mild toxicity to normal tissues.
31558017	2	78	from	penetration	437:447	arg1	sites					533:537	tumor sites	527:537	tumor sites	527:537	However, beyond that inducible heat resistance of tumor cells, the discounted therapeutic effect of low temperature hyperthermia was also ascribed to poor penetration of exogenous light stimulation and low accumulation of photothermal agents in tumor sites.
31558017	4	79	theme	Ink	912:914	arg1	accumulation					896:907	increased accumulation	886:907	increased accumulation of Ink and AIPH	886:923	The designed ALG hydrogel was used to improve the therapeutic effect by increased accumulation of Ink and AIPH and avoid potential side-effects caused by the unexpected spread to the surrounding normal tissues.
31558017	3	80	theme	effective	787:795	arg1	treatment					803:811	effective tumor treatment	787:811	effective tumor treatment	787:811	Herein, we constructed a multifunctional in situ hydrogel of sodium alginate (ALG) via Ca2+ coordinated with ALG to encapsulate the photothermal agent of Ink and azo initiator of 2,2'-azobis[2-(2-imidazolin-2-yl)propane]dihydrochloride (AIPH) for effective tumor treatment.
31558017	2	81	theme	photothermal	504:515	arg1	agents					517:522	photothermal agents	504:522	photothermal agents	504:522	However, beyond that inducible heat resistance of tumor cells, the discounted therapeutic effect of low temperature hyperthermia was also ascribed to poor penetration of exogenous light stimulation and low accumulation of photothermal agents in tumor sites.
31558017	8	82	theme	strong	1547:1552	arg1	signals					1568:1574	strong photoacoustic signals	1547:1574	strong photoacoustic signals	1547:1574	Furthermore, the formulation could also exert strong photoacoustic signals, which were utilized to monitor the stability of the composite hydrogel.
31558017	0	83	theme	Free	10:13	arg1	Radicals					15:22	Localized Free Radicals	0:22	Localized Free Radicals Burst	0:28	Localized Free Radicals Burst Triggered by NIR-II Light for Augmented Low-Temperature Photothermal Therapy.
31558017	2	84	theme	poor	432:435	arg1	penetration					437:447	poor penetration	432:447	poor penetration of exogenous light stimulation	432:478	However, beyond that inducible heat resistance of tumor cells, the discounted therapeutic effect of low temperature hyperthermia was also ascribed to poor penetration of exogenous light stimulation and low accumulation of photothermal agents in tumor sites.
31558017	2	85	theme	therapeutic	360:370	arg1	effect					372:377	the discounted therapeutic effect	345:377	the discounted therapeutic effect of low temperature hyperthermia	345:409	However, beyond that inducible heat resistance of tumor cells, the discounted therapeutic effect of low temperature hyperthermia was also ascribed to poor penetration of exogenous light stimulation and low accumulation of photothermal agents in tumor sites.
31558017	5	86	theme	rapid	1155:1159	arg1	decomposition					1161:1173	rapid decomposition	1155:1173	rapid decomposition of AIPH to produce alkyl radicals	1155:1207	After injection, local low temperature stimulation was generated with near-infrared-II irradiation by a 1064 nm laser, triggering rapid decomposition of AIPH to produce alkyl radicals.
31558017	4	87	theme	surrounding	997:1007	arg1	tissues					1016:1022	the surrounding normal tissues	993:1022	the surrounding normal tissues	993:1022	The designed ALG hydrogel was used to improve the therapeutic effect by increased accumulation of Ink and AIPH and avoid potential side-effects caused by the unexpected spread to the surrounding normal tissues.
31558017	2	88	theme	light	462:466	arg1	stimulation					468:478	exogenous light stimulation	452:478	exogenous light stimulation	452:478	However, beyond that inducible heat resistance of tumor cells, the discounted therapeutic effect of low temperature hyperthermia was also ascribed to poor penetration of exogenous light stimulation and low accumulation of photothermal agents in tumor sites.
31558017	2	89	theme	temperature	386:396	arg1	hyperthermia					398:409	low temperature hyperthermia	382:409	low temperature hyperthermia	382:409	However, beyond that inducible heat resistance of tumor cells, the discounted therapeutic effect of low temperature hyperthermia was also ascribed to poor penetration of exogenous light stimulation and low accumulation of photothermal agents in tumor sites.
31558017	5	90	theme	nm	1134:1135	arg1	laser					1137:1141	a 1064 nm laser	1127:1141	a 1064 nm laser	1127:1141	After injection, local low temperature stimulation was generated with near-infrared-II irradiation by a 1064 nm laser, triggering rapid decomposition of AIPH to produce alkyl radicals.
31558017	4	91	theme	unexpected	972:981	arg1	spread					983:988	the unexpected spread	968:988	the unexpected spread to the surrounding normal tissues	968:1022	The designed ALG hydrogel was used to improve the therapeutic effect by increased accumulation of Ink and AIPH and avoid potential side-effects caused by the unexpected spread to the surrounding normal tissues.
31558017	2	92	theme	heat	313:316	arg1	resistance					318:327	that inducible heat resistance	298:327	that inducible heat resistance of tumor cells	298:342	However, beyond that inducible heat resistance of tumor cells, the discounted therapeutic effect of low temperature hyperthermia was also ascribed to poor penetration of exogenous light stimulation and low accumulation of photothermal agents in tumor sites.
31558017	7	93	with	cancer	1464:1469	arg1	effects					1492:1498	negligible side effects	1476:1498	negligible side effects	1476:1498	Thus, remarkable inhibition of tumor growth was observed in a subcutaneous colorectal cancer with negligible side effects.
31558017	1	94	theme	damage	224:229	arg1	cells					238:242	damage cancer cells	224:242	damage cancer cells	224:242	As a novel treatment modality of tumors, hypothermal hyperthermia employed relatively lower temperature (<45 °C) to damage cancer cells with mild toxicity to normal tissues.
31558017	6	95	dep	temperature	1230:1240	arg1	therapy					1255:1261	photothermal therapy	1242:1261	photothermal therapy	1242:1261	The synergistic low temperature photothermal therapy and cytotoxic-free radicals enhanced the apoptosis of tumor cells via physical heat damage and lipid peroxidation.
30815757	0	0	theme	Insulin	99:105	arg1	Efficacy					87:94	the Therapeutic Efficacy	71:94	the Therapeutic Efficacy of Insulin	71:105	Polyglutamic Acid Functionalization of Chitosan Nanoparticles Enhances the Therapeutic Efficacy of Insulin Following Oral Administration.
30815757	2	1	theme	composite	603:611	arg1	design					613:618	a three-level central composite design	581:618	a three-level central composite design	581:618	Formulation was optimized by the design of experiments (DoE) approach using a three-level central composite design and characterized for in vitro parameters such as morphology, particle size, polydispersity index (PDI), entrapment efficiency and zeta potential.
30815757	1	2	theme	chitosan	167:174	arg1	Ch-NPs					191:196	Ch-NPs	191:196	Ch-NPs	191:196	In the present study, stable chitosan nanoparticles (Ch-NPs) were developed using the ionotropic gelation method, where poly(sodium 4-styrenesulfonate) (PSS) was used as a cross-linking agent while polyglutamic acid (PGA) for functionalization to improve the oral uptake through calcium-sensing receptors and amino acid transporters present in intestinal epithelium.
30815757	1	2	theme	chitosan	167:174	arg1	nanoparticles					176:188	stable chitosan nanoparticles	160:188	stable chitosan nanoparticles (Ch-NPs)	160:197	In the present study, stable chitosan nanoparticles (Ch-NPs) were developed using the ionotropic gelation method, where poly(sodium 4-styrenesulfonate) (PSS) was used as a cross-linking agent while polyglutamic acid (PGA) for functionalization to improve the oral uptake through calcium-sensing receptors and amino acid transporters present in intestinal epithelium.
30815757	2	3	dep	in	642:643	arg1	vitro					645:649	vitro	645:649	vitro	645:649	Formulation was optimized by the design of experiments (DoE) approach using a three-level central composite design and characterized for in vitro parameters such as morphology, particle size, polydispersity index (PDI), entrapment efficiency and zeta potential.
30815757	1	4	used	used	300:303	arg2	4-styrenesulfonate					270:287	sodium 4-styrenesulfonate	263:287	sodium 4-styrenesulfonate	263:287	In the present study, stable chitosan nanoparticles (Ch-NPs) were developed using the ionotropic gelation method, where poly(sodium 4-styrenesulfonate) (PSS) was used as a cross-linking agent while polyglutamic acid (PGA) for functionalization to improve the oral uptake through calcium-sensing receptors and amino acid transporters present in intestinal epithelium.
30815757	1	4	used	used	300:303	arg2	poly					258:261	poly	258:261	poly(sodium 4-styrenesulfonate) (PSS)	258:294	In the present study, stable chitosan nanoparticles (Ch-NPs) were developed using the ionotropic gelation method, where poly(sodium 4-styrenesulfonate) (PSS) was used as a cross-linking agent while polyglutamic acid (PGA) for functionalization to improve the oral uptake through calcium-sensing receptors and amino acid transporters present in intestinal epithelium.
30815757	1	4	used	used	300:303	arg2	agent					324:328	a cross-linking agent	308:328	a cross-linking agent while polyglutamic acid (PGA) for functionalization	308:380	In the present study, stable chitosan nanoparticles (Ch-NPs) were developed using the ionotropic gelation method, where poly(sodium 4-styrenesulfonate) (PSS) was used as a cross-linking agent while polyglutamic acid (PGA) for functionalization to improve the oral uptake through calcium-sensing receptors and amino acid transporters present in intestinal epithelium.
30815757	1	4	used	used	300:303	arg2	PSS					291:293	PSS	291:293	PSS	291:293	In the present study, stable chitosan nanoparticles (Ch-NPs) were developed using the ionotropic gelation method, where poly(sodium 4-styrenesulfonate) (PSS) was used as a cross-linking agent while polyglutamic acid (PGA) for functionalization to improve the oral uptake through calcium-sensing receptors and amino acid transporters present in intestinal epithelium.
30815757	2	5	theme	central	595:601	arg1	design					613:618	a three-level central composite design	581:618	a three-level central composite design	581:618	Formulation was optimized by the design of experiments (DoE) approach using a three-level central composite design and characterized for in vitro parameters such as morphology, particle size, polydispersity index (PDI), entrapment efficiency and zeta potential.
30815757	1	6	theme	calcium-sensing	417:431	arg1	receptors					433:441	calcium-sensing receptors	417:441	calcium-sensing receptors	417:441	In the present study, stable chitosan nanoparticles (Ch-NPs) were developed using the ionotropic gelation method, where poly(sodium 4-styrenesulfonate) (PSS) was used as a cross-linking agent while polyglutamic acid (PGA) for functionalization to improve the oral uptake through calcium-sensing receptors and amino acid transporters present in intestinal epithelium.
30815757	3	7	theme	particle	839:846	arg1	size					848:851	particle size	839:851	particle size	839:851	Morphological analysis demonstrated the formation of spherical NPs with particle size, zeta potential, and entrapment efficiency in the range of 210 nm ± 2.8 nm, 18.1 mV ± 0.14 mV, and 85.9% ± 0.28%, respectively.
30815757	0	8	theme	Oral	117:120	arg1	Administration					122:135	Oral Administration	117:135	Oral Administration	117:135	Polyglutamic Acid Functionalization of Chitosan Nanoparticles Enhances the Therapeutic Efficacy of Insulin Following Oral Administration.
30815757	1	9	theme	polyglutamic	336:347	arg1	PGA					355:357	PGA	355:357	PGA	355:357	In the present study, stable chitosan nanoparticles (Ch-NPs) were developed using the ionotropic gelation method, where poly(sodium 4-styrenesulfonate) (PSS) was used as a cross-linking agent while polyglutamic acid (PGA) for functionalization to improve the oral uptake through calcium-sensing receptors and amino acid transporters present in intestinal epithelium.
30815757	1	9	theme	polyglutamic	336:347	arg1	acid					349:352	polyglutamic acid	336:352	polyglutamic acid (PGA)	336:358	In the present study, stable chitosan nanoparticles (Ch-NPs) were developed using the ionotropic gelation method, where poly(sodium 4-styrenesulfonate) (PSS) was used as a cross-linking agent while polyglutamic acid (PGA) for functionalization to improve the oral uptake through calcium-sensing receptors and amino acid transporters present in intestinal epithelium.
30815757	2	10	theme	three-level	583:593	arg1	design					613:618	a three-level central composite design	581:618	a three-level central composite design	581:618	Formulation was optimized by the design of experiments (DoE) approach using a three-level central composite design and characterized for in vitro parameters such as morphology, particle size, polydispersity index (PDI), entrapment efficiency and zeta potential.
30815757	6	11	theme	poor	1599:1602	arg1	efficacy					1616:1623	poor therapeutic efficacy	1599:1623	poor therapeutic efficacy following oral administration	1599:1653	Data on hand suggest the ability of the developed NPs in overcoming the poor stability and, thus, poor therapeutic efficacy following oral administration.
30815757	6	11	theme	poor	1599:1602	arg1	stability					1578:1586	the poor stability	1569:1586	the poor stability	1569:1586	Data on hand suggest the ability of the developed NPs in overcoming the poor stability and, thus, poor therapeutic efficacy following oral administration.
30815757	1	12	theme	sodium	263:268	arg1	poly					258:261	poly	258:261	poly(sodium 4-styrenesulfonate) (PSS)	258:294	In the present study, stable chitosan nanoparticles (Ch-NPs) were developed using the ionotropic gelation method, where poly(sodium 4-styrenesulfonate) (PSS) was used as a cross-linking agent while polyglutamic acid (PGA) for functionalization to improve the oral uptake through calcium-sensing receptors and amino acid transporters present in intestinal epithelium.
30815757	1	12	theme	sodium	263:268	arg1	4-styrenesulfonate					270:287	sodium 4-styrenesulfonate	263:287	sodium 4-styrenesulfonate	263:287	In the present study, stable chitosan nanoparticles (Ch-NPs) were developed using the ionotropic gelation method, where poly(sodium 4-styrenesulfonate) (PSS) was used as a cross-linking agent while polyglutamic acid (PGA) for functionalization to improve the oral uptake through calcium-sensing receptors and amino acid transporters present in intestinal epithelium.
30815757	6	13	theme	developed	1541:1549	arg1	NPs					1551:1553	the developed NPs	1537:1553	the developed NPs	1537:1553	Data on hand suggest the ability of the developed NPs in overcoming the poor stability and, thus, poor therapeutic efficacy following oral administration.
30815757	5	14	dep	In	1162:1163	arg1	vivo					1165:1168	vivo	1165:1168	vivo	1165:1168	In vivo studies in diabetic animals demonstrated low levels of plasma glucose for almost 24 h. Pharmacological availability (PA) of insulin administered through Ch-PSS-PGA NPs (17.28 ± 0.9) was significantly higher as compared to that of insulin administered through control NPs, i.e., Ch-PGA NPs (10.9 ± 1.5) and Ch-PSS NPs (12.9 ± 1.8).
30815757	4	15	from	conditions	1043:1052	arg1	release					1019:1025	sustained release	1009:1025	sustained release at different pH conditions	1009:1052	The developed NPs exhibited sustained release at different pH conditions and almost threefold higher uptake in comparison with non-functionalized NPs in Caco-2 cell uptake studies.
30815757	4	15	from	conditions	1043:1052	arg1	uptake					1082:1087	almost threefold higher uptake	1058:1087	almost threefold higher uptake	1058:1087	The developed NPs exhibited sustained release at different pH conditions and almost threefold higher uptake in comparison with non-functionalized NPs in Caco-2 cell uptake studies.
30815757	2	16	theme	zeta	751:754	arg1	potential					756:764	zeta potential	751:764	zeta potential	751:764	Formulation was optimized by the design of experiments (DoE) approach using a three-level central composite design and characterized for in vitro parameters such as morphology, particle size, polydispersity index (PDI), entrapment efficiency and zeta potential.
30815757	3	17	theme	NPs	830:832	arg1	formation					807:815	the formation	803:815	the formation of spherical NPs	803:832	Morphological analysis demonstrated the formation of spherical NPs with particle size, zeta potential, and entrapment efficiency in the range of 210 nm ± 2.8 nm, 18.1 mV ± 0.14 mV, and 85.9% ± 0.28%, respectively.
30815757	4	18	theme	Caco-2	1134:1139	arg1	studies					1153:1159	Caco-2 cell uptake studies	1134:1159	Caco-2 cell uptake studies	1134:1159	The developed NPs exhibited sustained release at different pH conditions and almost threefold higher uptake in comparison with non-functionalized NPs in Caco-2 cell uptake studies.
30815757	1	19	theme	amino	447:451	arg1	transporters					458:469	amino acid transporters	447:469	amino acid transporters	447:469	In the present study, stable chitosan nanoparticles (Ch-NPs) were developed using the ionotropic gelation method, where poly(sodium 4-styrenesulfonate) (PSS) was used as a cross-linking agent while polyglutamic acid (PGA) for functionalization to improve the oral uptake through calcium-sensing receptors and amino acid transporters present in intestinal epithelium.
30815757	5	20	theme	Pharmacological	1257:1271	arg1	availability					1273:1284	almost 24 h. Pharmacological availability	1244:1284	almost 24 h. Pharmacological availability (PA) of insulin administered through Ch-PSS-PGA NPs (17.28 ± 0.9)	1244:1350	In vivo studies in diabetic animals demonstrated low levels of plasma glucose for almost 24 h. Pharmacological availability (PA) of insulin administered through Ch-PSS-PGA NPs (17.28 ± 0.9) was significantly higher as compared to that of insulin administered through control NPs, i.e., Ch-PGA NPs (10.9 ± 1.5) and Ch-PSS NPs (12.9 ± 1.8).
30815757	5	20	theme	Pharmacological	1257:1271	arg1	PA					1287:1288	PA	1287:1288	PA	1287:1288	In vivo studies in diabetic animals demonstrated low levels of plasma glucose for almost 24 h. Pharmacological availability (PA) of insulin administered through Ch-PSS-PGA NPs (17.28 ± 0.9) was significantly higher as compared to that of insulin administered through control NPs, i.e., Ch-PGA NPs (10.9 ± 1.5) and Ch-PSS NPs (12.9 ± 1.8).
30815757	3	21	theme	spherical	820:828	arg1	NPs					830:832	spherical NPs	820:832	spherical NPs	820:832	Morphological analysis demonstrated the formation of spherical NPs with particle size, zeta potential, and entrapment efficiency in the range of 210 nm ± 2.8 nm, 18.1 mV ± 0.14 mV, and 85.9% ± 0.28%, respectively.
30815757	4	22	theme	developed	985:993	arg1	NPs					995:997	The developed NPs	981:997	The developed NPs	981:997	The developed NPs exhibited sustained release at different pH conditions and almost threefold higher uptake in comparison with non-functionalized NPs in Caco-2 cell uptake studies.
30815757	1	23	theme	acid	453:456	arg1	transporters					458:469	amino acid transporters	447:469	amino acid transporters	447:469	In the present study, stable chitosan nanoparticles (Ch-NPs) were developed using the ionotropic gelation method, where poly(sodium 4-styrenesulfonate) (PSS) was used as a cross-linking agent while polyglutamic acid (PGA) for functionalization to improve the oral uptake through calcium-sensing receptors and amino acid transporters present in intestinal epithelium.
30815757	4	24	theme	sustained	1009:1017	arg1	release					1019:1025	sustained release	1009:1025	sustained release at different pH conditions	1009:1052	The developed NPs exhibited sustained release at different pH conditions and almost threefold higher uptake in comparison with non-functionalized NPs in Caco-2 cell uptake studies.
30815757	4	25	theme	uptake	1146:1151	arg1	studies					1153:1159	Caco-2 cell uptake studies	1134:1159	Caco-2 cell uptake studies	1134:1159	The developed NPs exhibited sustained release at different pH conditions and almost threefold higher uptake in comparison with non-functionalized NPs in Caco-2 cell uptake studies.
30815757	0	26	theme	Acid	13:16	arg1	Functionalization					18:34	Polyglutamic Acid Functionalization	0:34	Polyglutamic Acid Functionalization of Chitosan Nanoparticles	0:60	Polyglutamic Acid Functionalization of Chitosan Nanoparticles Enhances the Therapeutic Efficacy of Insulin Following Oral Administration.
30815757	5	27	from	studies	1170:1176	arg1	animals					1190:1196	diabetic animals	1181:1196	diabetic animals	1181:1196	In vivo studies in diabetic animals demonstrated low levels of plasma glucose for almost 24 h. Pharmacological availability (PA) of insulin administered through Ch-PSS-PGA NPs (17.28 ± 0.9) was significantly higher as compared to that of insulin administered through control NPs, i.e., Ch-PGA NPs (10.9 ± 1.5) and Ch-PSS NPs (12.9 ± 1.8).
30815757	5	28	theme	Ch-PGA	1448:1453	arg1	10.9 ± 1.5					1460:1469	10.9 ± 1.5	1460:1469	10.9 ± 1.5	1460:1469	In vivo studies in diabetic animals demonstrated low levels of plasma glucose for almost 24 h. Pharmacological availability (PA) of insulin administered through Ch-PSS-PGA NPs (17.28 ± 0.9) was significantly higher as compared to that of insulin administered through control NPs, i.e., Ch-PGA NPs (10.9 ± 1.5) and Ch-PSS NPs (12.9 ± 1.8).
30815757	5	28	theme	Ch-PGA	1448:1453	arg1	NPs					1455:1457	Ch-PGA NPs	1448:1457	Ch-PGA NPs (10.9 ± 1.5)	1448:1470	In vivo studies in diabetic animals demonstrated low levels of plasma glucose for almost 24 h. Pharmacological availability (PA) of insulin administered through Ch-PSS-PGA NPs (17.28 ± 0.9) was significantly higher as compared to that of insulin administered through control NPs, i.e., Ch-PGA NPs (10.9 ± 1.5) and Ch-PSS NPs (12.9 ± 1.8).
30815757	2	29	theme	entrapment	725:734	arg1	efficiency					736:745	entrapment efficiency	725:745	entrapment efficiency	725:745	Formulation was optimized by the design of experiments (DoE) approach using a three-level central composite design and characterized for in vitro parameters such as morphology, particle size, polydispersity index (PDI), entrapment efficiency and zeta potential.
30815757	0	30	theme	Polyglutamic	0:11	arg1	Functionalization					18:34	Polyglutamic Acid Functionalization	0:34	Polyglutamic Acid Functionalization of Chitosan Nanoparticles	0:60	Polyglutamic Acid Functionalization of Chitosan Nanoparticles Enhances the Therapeutic Efficacy of Insulin Following Oral Administration.
30815757	1	31	theme	present	471:477	arg1	receptors					433:441	calcium-sensing receptors	417:441	calcium-sensing receptors	417:441	In the present study, stable chitosan nanoparticles (Ch-NPs) were developed using the ionotropic gelation method, where poly(sodium 4-styrenesulfonate) (PSS) was used as a cross-linking agent while polyglutamic acid (PGA) for functionalization to improve the oral uptake through calcium-sensing receptors and amino acid transporters present in intestinal epithelium.
30815757	3	32	theme	18.1 mV ± 0.14 mV	929:945	arg1	range					903:907	the range	899:907	the range of 210 nm ± 2.8 nm, 18.1 mV ± 0.14 mV, and 85.9% ± 0.28%, respectively	899:978	Morphological analysis demonstrated the formation of spherical NPs with particle size, zeta potential, and entrapment efficiency in the range of 210 nm ± 2.8 nm, 18.1 mV ± 0.14 mV, and 85.9% ± 0.28%, respectively.
30815757	5	33	theme	Ch-PSS	1476:1481	arg1	12.9 ± 1.8					1488:1497	12.9 ± 1.8	1488:1497	12.9 ± 1.8	1488:1497	In vivo studies in diabetic animals demonstrated low levels of plasma glucose for almost 24 h. Pharmacological availability (PA) of insulin administered through Ch-PSS-PGA NPs (17.28 ± 0.9) was significantly higher as compared to that of insulin administered through control NPs, i.e., Ch-PGA NPs (10.9 ± 1.5) and Ch-PSS NPs (12.9 ± 1.8).
30815757	5	33	theme	Ch-PSS	1476:1481	arg1	NPs					1483:1485	Ch-PSS NPs	1476:1485	Ch-PSS NPs (12.9 ± 1.8)	1476:1498	In vivo studies in diabetic animals demonstrated low levels of plasma glucose for almost 24 h. Pharmacological availability (PA) of insulin administered through Ch-PSS-PGA NPs (17.28 ± 0.9) was significantly higher as compared to that of insulin administered through control NPs, i.e., Ch-PGA NPs (10.9 ± 1.5) and Ch-PSS NPs (12.9 ± 1.8).
30815757	4	34	theme	different	1030:1038	arg1	conditions					1043:1052	different pH conditions	1030:1052	different pH conditions	1030:1052	The developed NPs exhibited sustained release at different pH conditions and almost threefold higher uptake in comparison with non-functionalized NPs in Caco-2 cell uptake studies.
30815757	5	35	theme	control	1429:1435	arg1	NPs					1437:1439	control NPs	1429:1439	control NPs	1429:1439	In vivo studies in diabetic animals demonstrated low levels of plasma glucose for almost 24 h. Pharmacological availability (PA) of insulin administered through Ch-PSS-PGA NPs (17.28 ± 0.9) was significantly higher as compared to that of insulin administered through control NPs, i.e., Ch-PGA NPs (10.9 ± 1.5) and Ch-PSS NPs (12.9 ± 1.8).
30815757	2	36	theme	in	642:643	arg1	potential					756:764	zeta potential	751:764	zeta potential	751:764	Formulation was optimized by the design of experiments (DoE) approach using a three-level central composite design and characterized for in vitro parameters such as morphology, particle size, polydispersity index (PDI), entrapment efficiency and zeta potential.
30815757	2	36	theme	in	642:643	arg1	parameters					651:660	in vitro parameters	642:660	in vitro parameters such as morphology, particle size, polydispersity index (PDI), entrapment efficiency and zeta potential	642:764	Formulation was optimized by the design of experiments (DoE) approach using a three-level central composite design and characterized for in vitro parameters such as morphology, particle size, polydispersity index (PDI), entrapment efficiency and zeta potential.
30815757	2	36	theme	in	642:643	arg1	size					691:694	particle size	682:694	particle size	682:694	Formulation was optimized by the design of experiments (DoE) approach using a three-level central composite design and characterized for in vitro parameters such as morphology, particle size, polydispersity index (PDI), entrapment efficiency and zeta potential.
30815757	2	36	theme	in	642:643	arg1	index					712:716	polydispersity index	697:716	polydispersity index (PDI)	697:722	Formulation was optimized by the design of experiments (DoE) approach using a three-level central composite design and characterized for in vitro parameters such as morphology, particle size, polydispersity index (PDI), entrapment efficiency and zeta potential.
30815757	2	36	theme	in	642:643	arg1	efficiency					736:745	entrapment efficiency	725:745	entrapment efficiency	725:745	Formulation was optimized by the design of experiments (DoE) approach using a three-level central composite design and characterized for in vitro parameters such as morphology, particle size, polydispersity index (PDI), entrapment efficiency and zeta potential.
30815757	2	36	theme	in	642:643	arg1	morphology					670:679	morphology	670:679	morphology	670:679	Formulation was optimized by the design of experiments (DoE) approach using a three-level central composite design and characterized for in vitro parameters such as morphology, particle size, polydispersity index (PDI), entrapment efficiency and zeta potential.
30815757	6	37	from	Data	1501:1504	arg1	hand					1509:1512	hand	1509:1512	hand	1509:1512	Data on hand suggest the ability of the developed NPs in overcoming the poor stability and, thus, poor therapeutic efficacy following oral administration.
30815757	6	38	theme	therapeutic	1604:1614	arg1	efficacy					1616:1623	poor therapeutic efficacy	1599:1623	poor therapeutic efficacy following oral administration	1599:1653	Data on hand suggest the ability of the developed NPs in overcoming the poor stability and, thus, poor therapeutic efficacy following oral administration.
30815757	6	38	theme	therapeutic	1604:1614	arg1	stability					1578:1586	the poor stability	1569:1586	the poor stability	1569:1586	Data on hand suggest the ability of the developed NPs in overcoming the poor stability and, thus, poor therapeutic efficacy following oral administration.
30815757	4	39	from	NPs	1127:1129	arg1	studies					1153:1159	Caco-2 cell uptake studies	1134:1159	Caco-2 cell uptake studies	1134:1159	The developed NPs exhibited sustained release at different pH conditions and almost threefold higher uptake in comparison with non-functionalized NPs in Caco-2 cell uptake studies.
30815757	5	40	theme	diabetic	1181:1188	arg1	animals					1190:1196	diabetic animals	1181:1196	diabetic animals	1181:1196	In vivo studies in diabetic animals demonstrated low levels of plasma glucose for almost 24 h. Pharmacological availability (PA) of insulin administered through Ch-PSS-PGA NPs (17.28 ± 0.9) was significantly higher as compared to that of insulin administered through control NPs, i.e., Ch-PGA NPs (10.9 ± 1.5) and Ch-PSS NPs (12.9 ± 1.8).
30815757	2	41	theme	experiments	548:558	arg1	approach					566:573	experiments (DoE) approach	548:573	experiments (DoE) approach using a three-level central composite design	548:618	Formulation was optimized by the design of experiments (DoE) approach using a three-level central composite design and characterized for in vitro parameters such as morphology, particle size, polydispersity index (PDI), entrapment efficiency and zeta potential.
30815757	5	42	dep	demonstrated	1198:1209	arg1	higher					1370:1375	higher	1370:1375	higher	1370:1375	In vivo studies in diabetic animals demonstrated low levels of plasma glucose for almost 24 h. Pharmacological availability (PA) of insulin administered through Ch-PSS-PGA NPs (17.28 ± 0.9) was significantly higher as compared to that of insulin administered through control NPs, i.e., Ch-PGA NPs (10.9 ± 1.5) and Ch-PSS NPs (12.9 ± 1.8).
30815757	3	43	theme	210 nm ± 2.8 nm	912:926	arg1	range					903:907	the range	899:907	the range of 210 nm ± 2.8 nm, 18.1 mV ± 0.14 mV, and 85.9% ± 0.28%, respectively	899:978	Morphological analysis demonstrated the formation of spherical NPs with particle size, zeta potential, and entrapment efficiency in the range of 210 nm ± 2.8 nm, 18.1 mV ± 0.14 mV, and 85.9% ± 0.28%, respectively.
30815757	0	44	theme	Nanoparticles	48:60	arg1	Functionalization					18:34	Polyglutamic Acid Functionalization	0:34	Polyglutamic Acid Functionalization of Chitosan Nanoparticles	0:60	Polyglutamic Acid Functionalization of Chitosan Nanoparticles Enhances the Therapeutic Efficacy of Insulin Following Oral Administration.
30815757	5	45	theme	plasma	1225:1230	arg1	glucose					1232:1238	plasma glucose	1225:1238	plasma glucose	1225:1238	In vivo studies in diabetic animals demonstrated low levels of plasma glucose for almost 24 h. Pharmacological availability (PA) of insulin administered through Ch-PSS-PGA NPs (17.28 ± 0.9) was significantly higher as compared to that of insulin administered through control NPs, i.e., Ch-PGA NPs (10.9 ± 1.5) and Ch-PSS NPs (12.9 ± 1.8).
30815757	4	46	theme	non-functionalized	1108:1125	arg1	NPs					1127:1129	non-functionalized NPs	1108:1129	non-functionalized NPs in Caco-2 cell uptake studies	1108:1159	The developed NPs exhibited sustained release at different pH conditions and almost threefold higher uptake in comparison with non-functionalized NPs in Caco-2 cell uptake studies.
30815757	0	47	theme	Chitosan	39:46	arg1	Nanoparticles					48:60	Chitosan Nanoparticles	39:60	Chitosan Nanoparticles	39:60	Polyglutamic Acid Functionalization of Chitosan Nanoparticles Enhances the Therapeutic Efficacy of Insulin Following Oral Administration.
30815757	3	48	theme	Morphological	767:779	arg1	analysis					781:788	Morphological analysis	767:788	Morphological analysis	767:788	Morphological analysis demonstrated the formation of spherical NPs with particle size, zeta potential, and entrapment efficiency in the range of 210 nm ± 2.8 nm, 18.1 mV ± 0.14 mV, and 85.9% ± 0.28%, respectively.
30815757	5	49	theme	low	1211:1213	arg1	levels					1215:1220	low levels	1211:1220	low levels of plasma glucose for almost 24 h. Pharmacological availability (PA) of insulin administered through Ch-PSS-PGA NPs (17.28 ± 0.9)	1211:1350	In vivo studies in diabetic animals demonstrated low levels of plasma glucose for almost 24 h. Pharmacological availability (PA) of insulin administered through Ch-PSS-PGA NPs (17.28 ± 0.9) was significantly higher as compared to that of insulin administered through control NPs, i.e., Ch-PGA NPs (10.9 ± 1.5) and Ch-PSS NPs (12.9 ± 1.8).
30815757	5	50	theme	Ch-PSS-PGA	1323:1332	arg1	17.28 ± 0.9					1339:1349	17.28 ± 0.9	1339:1349	17.28 ± 0.9	1339:1349	In vivo studies in diabetic animals demonstrated low levels of plasma glucose for almost 24 h. Pharmacological availability (PA) of insulin administered through Ch-PSS-PGA NPs (17.28 ± 0.9) was significantly higher as compared to that of insulin administered through control NPs, i.e., Ch-PGA NPs (10.9 ± 1.5) and Ch-PSS NPs (12.9 ± 1.8).
30815757	5	50	theme	Ch-PSS-PGA	1323:1332	arg1	NPs					1334:1336	Ch-PSS-PGA NPs	1323:1336	Ch-PSS-PGA NPs (17.28 ± 0.9)	1323:1350	In vivo studies in diabetic animals demonstrated low levels of plasma glucose for almost 24 h. Pharmacological availability (PA) of insulin administered through Ch-PSS-PGA NPs (17.28 ± 0.9) was significantly higher as compared to that of insulin administered through control NPs, i.e., Ch-PGA NPs (10.9 ± 1.5) and Ch-PSS NPs (12.9 ± 1.8).
30815757	1	51	theme	intestinal	482:491	arg1	epithelium					493:502	intestinal epithelium	482:502	intestinal epithelium	482:502	In the present study, stable chitosan nanoparticles (Ch-NPs) were developed using the ionotropic gelation method, where poly(sodium 4-styrenesulfonate) (PSS) was used as a cross-linking agent while polyglutamic acid (PGA) for functionalization to improve the oral uptake through calcium-sensing receptors and amino acid transporters present in intestinal epithelium.
30815757	1	52	theme	ionotropic	224:233	arg1	method					244:249	the ionotropic gelation method	220:249	the ionotropic gelation method	220:249	In the present study, stable chitosan nanoparticles (Ch-NPs) were developed using the ionotropic gelation method, where poly(sodium 4-styrenesulfonate) (PSS) was used as a cross-linking agent while polyglutamic acid (PGA) for functionalization to improve the oral uptake through calcium-sensing receptors and amino acid transporters present in intestinal epithelium.
30815757	5	53	dep	NPs	1455:1457	arg1	i.e.					1442:1445	i.e.	1442:1445	i.e.	1442:1445	In vivo studies in diabetic animals demonstrated low levels of plasma glucose for almost 24 h. Pharmacological availability (PA) of insulin administered through Ch-PSS-PGA NPs (17.28 ± 0.9) was significantly higher as compared to that of insulin administered through control NPs, i.e., Ch-PGA NPs (10.9 ± 1.5) and Ch-PSS NPs (12.9 ± 1.8).
30815757	2	54	theme	particle	682:689	arg1	size					691:694	particle size	682:694	particle size	682:694	Formulation was optimized by the design of experiments (DoE) approach using a three-level central composite design and characterized for in vitro parameters such as morphology, particle size, polydispersity index (PDI), entrapment efficiency and zeta potential.
30815757	2	55	theme	polydispersity	697:710	arg1	PDI					719:721	PDI	719:721	PDI	719:721	Formulation was optimized by the design of experiments (DoE) approach using a three-level central composite design and characterized for in vitro parameters such as morphology, particle size, polydispersity index (PDI), entrapment efficiency and zeta potential.
30815757	2	55	theme	polydispersity	697:710	arg1	index					712:716	polydispersity index	697:716	polydispersity index (PDI)	697:722	Formulation was optimized by the design of experiments (DoE) approach using a three-level central composite design and characterized for in vitro parameters such as morphology, particle size, polydispersity index (PDI), entrapment efficiency and zeta potential.
30815757	1	56	from	present	471:477	arg1	epithelium					493:502	intestinal epithelium	482:502	intestinal epithelium	482:502	In the present study, stable chitosan nanoparticles (Ch-NPs) were developed using the ionotropic gelation method, where poly(sodium 4-styrenesulfonate) (PSS) was used as a cross-linking agent while polyglutamic acid (PGA) for functionalization to improve the oral uptake through calcium-sensing receptors and amino acid transporters present in intestinal epithelium.
30815757	1	57	attach	present	471:477	arg1	epithelium					493:502	intestinal epithelium	482:502	intestinal epithelium	482:502	In the present study, stable chitosan nanoparticles (Ch-NPs) were developed using the ionotropic gelation method, where poly(sodium 4-styrenesulfonate) (PSS) was used as a cross-linking agent while polyglutamic acid (PGA) for functionalization to improve the oral uptake through calcium-sensing receptors and amino acid transporters present in intestinal epithelium.
30815757	1	57	attach	present	471:477	arg2	receptors					433:441	calcium-sensing receptors	417:441	calcium-sensing receptors	417:441	In the present study, stable chitosan nanoparticles (Ch-NPs) were developed using the ionotropic gelation method, where poly(sodium 4-styrenesulfonate) (PSS) was used as a cross-linking agent while polyglutamic acid (PGA) for functionalization to improve the oral uptake through calcium-sensing receptors and amino acid transporters present in intestinal epithelium.
30815757	5	58	theme	In	1162:1163	arg1	studies					1170:1176	In vivo studies	1162:1176	In vivo studies in diabetic animals	1162:1196	In vivo studies in diabetic animals demonstrated low levels of plasma glucose for almost 24 h. Pharmacological availability (PA) of insulin administered through Ch-PSS-PGA NPs (17.28 ± 0.9) was significantly higher as compared to that of insulin administered through control NPs, i.e., Ch-PGA NPs (10.9 ± 1.5) and Ch-PSS NPs (12.9 ± 1.8).
30815757	2	59	theme	approach	566:573	arg1	design					538:543	the design	534:543	the design of experiments (DoE) approach using a three-level central composite design	534:618	Formulation was optimized by the design of experiments (DoE) approach using a three-level central composite design and characterized for in vitro parameters such as morphology, particle size, polydispersity index (PDI), entrapment efficiency and zeta potential.
30815757	6	60	theme	oral	1635:1638	arg1	administration					1640:1653	oral administration	1635:1653	oral administration	1635:1653	Data on hand suggest the ability of the developed NPs in overcoming the poor stability and, thus, poor therapeutic efficacy following oral administration.
30815757	3	61	theme	zeta	854:857	arg1	potential					859:867	zeta potential	854:867	zeta potential	854:867	Morphological analysis demonstrated the formation of spherical NPs with particle size, zeta potential, and entrapment efficiency in the range of 210 nm ± 2.8 nm, 18.1 mV ± 0.14 mV, and 85.9% ± 0.28%, respectively.
30815757	6	62	theme	poor	1573:1576	arg1	efficacy					1616:1623	poor therapeutic efficacy	1599:1623	poor therapeutic efficacy following oral administration	1599:1653	Data on hand suggest the ability of the developed NPs in overcoming the poor stability and, thus, poor therapeutic efficacy following oral administration.
30815757	6	62	theme	poor	1573:1576	arg1	stability					1578:1586	the poor stability	1569:1586	the poor stability	1569:1586	Data on hand suggest the ability of the developed NPs in overcoming the poor stability and, thus, poor therapeutic efficacy following oral administration.
30815757	1	63	theme	oral	397:400	arg1	uptake					402:407	the oral uptake	393:407	the oral uptake through calcium-sensing receptors and amino acid transporters present in intestinal epithelium	393:502	In the present study, stable chitosan nanoparticles (Ch-NPs) were developed using the ionotropic gelation method, where poly(sodium 4-styrenesulfonate) (PSS) was used as a cross-linking agent while polyglutamic acid (PGA) for functionalization to improve the oral uptake through calcium-sensing receptors and amino acid transporters present in intestinal epithelium.
30815757	6	64	theme	NPs	1551:1553	arg1	ability					1526:1532	the ability	1522:1532	the ability of the developed NPs	1522:1553	Data on hand suggest the ability of the developed NPs in overcoming the poor stability and, thus, poor therapeutic efficacy following oral administration.
30815757	1	65	theme	present	145:151	arg1	study					153:157	the present study	141:157	the present study	141:157	In the present study, stable chitosan nanoparticles (Ch-NPs) were developed using the ionotropic gelation method, where poly(sodium 4-styrenesulfonate) (PSS) was used as a cross-linking agent while polyglutamic acid (PGA) for functionalization to improve the oral uptake through calcium-sensing receptors and amino acid transporters present in intestinal epithelium.
30815757	4	66	theme	higher	1075:1080	arg1	uptake					1082:1087	almost threefold higher uptake	1058:1087	almost threefold higher uptake	1058:1087	The developed NPs exhibited sustained release at different pH conditions and almost threefold higher uptake in comparison with non-functionalized NPs in Caco-2 cell uptake studies.
30815757	1	67	theme	gelation	235:242	arg1	method					244:249	the ionotropic gelation method	220:249	the ionotropic gelation method	220:249	In the present study, stable chitosan nanoparticles (Ch-NPs) were developed using the ionotropic gelation method, where poly(sodium 4-styrenesulfonate) (PSS) was used as a cross-linking agent while polyglutamic acid (PGA) for functionalization to improve the oral uptake through calcium-sensing receptors and amino acid transporters present in intestinal epithelium.
30815757	4	68	with	comparison	1092:1101	arg1	NPs					1127:1129	non-functionalized NPs	1108:1129	non-functionalized NPs in Caco-2 cell uptake studies	1108:1159	The developed NPs exhibited sustained release at different pH conditions and almost threefold higher uptake in comparison with non-functionalized NPs in Caco-2 cell uptake studies.
30815757	4	69	theme	pH	1040:1041	arg1	conditions					1043:1052	different pH conditions	1030:1052	different pH conditions	1030:1052	The developed NPs exhibited sustained release at different pH conditions and almost threefold higher uptake in comparison with non-functionalized NPs in Caco-2 cell uptake studies.
30815757	3	70	theme	%	956:956	arg1	%					964:964	85.9% ± 0.28%	952:964	85.9% ± 0.28%	952:964	Morphological analysis demonstrated the formation of spherical NPs with particle size, zeta potential, and entrapment efficiency in the range of 210 nm ± 2.8 nm, 18.1 mV ± 0.14 mV, and 85.9% ± 0.28%, respectively.
30815757	4	71	theme	cell	1141:1144	arg1	studies					1153:1159	Caco-2 cell uptake studies	1134:1159	Caco-2 cell uptake studies	1134:1159	The developed NPs exhibited sustained release at different pH conditions and almost threefold higher uptake in comparison with non-functionalized NPs in Caco-2 cell uptake studies.
30815757	5	72	theme	glucose	1232:1238	arg1	levels					1215:1220	low levels	1211:1220	low levels of plasma glucose for almost 24 h. Pharmacological availability (PA) of insulin administered through Ch-PSS-PGA NPs (17.28 ± 0.9)	1211:1350	In vivo studies in diabetic animals demonstrated low levels of plasma glucose for almost 24 h. Pharmacological availability (PA) of insulin administered through Ch-PSS-PGA NPs (17.28 ± 0.9) was significantly higher as compared to that of insulin administered through control NPs, i.e., Ch-PGA NPs (10.9 ± 1.5) and Ch-PSS NPs (12.9 ± 1.8).
30815757	1	73	from	epithelium	493:502	arg1	present					471:477	present	471:477	present	471:477	In the present study, stable chitosan nanoparticles (Ch-NPs) were developed using the ionotropic gelation method, where poly(sodium 4-styrenesulfonate) (PSS) was used as a cross-linking agent while polyglutamic acid (PGA) for functionalization to improve the oral uptake through calcium-sensing receptors and amino acid transporters present in intestinal epithelium.
30815757	5	74	theme	24 h.	1251:1255	arg1	availability					1273:1284	almost 24 h. Pharmacological availability	1244:1284	almost 24 h. Pharmacological availability (PA) of insulin administered through Ch-PSS-PGA NPs (17.28 ± 0.9)	1244:1350	In vivo studies in diabetic animals demonstrated low levels of plasma glucose for almost 24 h. Pharmacological availability (PA) of insulin administered through Ch-PSS-PGA NPs (17.28 ± 0.9) was significantly higher as compared to that of insulin administered through control NPs, i.e., Ch-PGA NPs (10.9 ± 1.5) and Ch-PSS NPs (12.9 ± 1.8).
30815757	5	74	theme	24 h.	1251:1255	arg1	PA					1287:1288	PA	1287:1288	PA	1287:1288	In vivo studies in diabetic animals demonstrated low levels of plasma glucose for almost 24 h. Pharmacological availability (PA) of insulin administered through Ch-PSS-PGA NPs (17.28 ± 0.9) was significantly higher as compared to that of insulin administered through control NPs, i.e., Ch-PGA NPs (10.9 ± 1.5) and Ch-PSS NPs (12.9 ± 1.8).
30815757	5	75	theme	insulin	1294:1300	arg1	availability					1273:1284	almost 24 h. Pharmacological availability	1244:1284	almost 24 h. Pharmacological availability (PA) of insulin administered through Ch-PSS-PGA NPs (17.28 ± 0.9)	1244:1350	In vivo studies in diabetic animals demonstrated low levels of plasma glucose for almost 24 h. Pharmacological availability (PA) of insulin administered through Ch-PSS-PGA NPs (17.28 ± 0.9) was significantly higher as compared to that of insulin administered through control NPs, i.e., Ch-PGA NPs (10.9 ± 1.5) and Ch-PSS NPs (12.9 ± 1.8).
30815757	5	75	theme	insulin	1294:1300	arg1	PA					1287:1288	PA	1287:1288	PA	1287:1288	In vivo studies in diabetic animals demonstrated low levels of plasma glucose for almost 24 h. Pharmacological availability (PA) of insulin administered through Ch-PSS-PGA NPs (17.28 ± 0.9) was significantly higher as compared to that of insulin administered through control NPs, i.e., Ch-PGA NPs (10.9 ± 1.5) and Ch-PSS NPs (12.9 ± 1.8).
30815757	0	76	theme	Therapeutic	75:85	arg1	Efficacy					87:94	the Therapeutic Efficacy	71:94	the Therapeutic Efficacy of Insulin	71:105	Polyglutamic Acid Functionalization of Chitosan Nanoparticles Enhances the Therapeutic Efficacy of Insulin Following Oral Administration.
30815757	1	77	theme	cross-linking	310:322	arg1	agent					324:328	a cross-linking agent	308:328	a cross-linking agent while polyglutamic acid (PGA) for functionalization	308:380	In the present study, stable chitosan nanoparticles (Ch-NPs) were developed using the ionotropic gelation method, where poly(sodium 4-styrenesulfonate) (PSS) was used as a cross-linking agent while polyglutamic acid (PGA) for functionalization to improve the oral uptake through calcium-sensing receptors and amino acid transporters present in intestinal epithelium.
30815757	1	77	theme	cross-linking	310:322	arg1	poly					258:261	poly	258:261	poly(sodium 4-styrenesulfonate) (PSS)	258:294	In the present study, stable chitosan nanoparticles (Ch-NPs) were developed using the ionotropic gelation method, where poly(sodium 4-styrenesulfonate) (PSS) was used as a cross-linking agent while polyglutamic acid (PGA) for functionalization to improve the oral uptake through calcium-sensing receptors and amino acid transporters present in intestinal epithelium.
30815757	3	78	theme	%	964:964	arg1	range					903:907	the range	899:907	the range of 210 nm ± 2.8 nm, 18.1 mV ± 0.14 mV, and 85.9% ± 0.28%, respectively	899:978	Morphological analysis demonstrated the formation of spherical NPs with particle size, zeta potential, and entrapment efficiency in the range of 210 nm ± 2.8 nm, 18.1 mV ± 0.14 mV, and 85.9% ± 0.28%, respectively.
30815757	1	79	theme	stable	160:165	arg1	Ch-NPs					191:196	Ch-NPs	191:196	Ch-NPs	191:196	In the present study, stable chitosan nanoparticles (Ch-NPs) were developed using the ionotropic gelation method, where poly(sodium 4-styrenesulfonate) (PSS) was used as a cross-linking agent while polyglutamic acid (PGA) for functionalization to improve the oral uptake through calcium-sensing receptors and amino acid transporters present in intestinal epithelium.
30815757	1	79	theme	stable	160:165	arg1	nanoparticles					176:188	stable chitosan nanoparticles	160:188	stable chitosan nanoparticles (Ch-NPs)	160:197	In the present study, stable chitosan nanoparticles (Ch-NPs) were developed using the ionotropic gelation method, where poly(sodium 4-styrenesulfonate) (PSS) was used as a cross-linking agent while polyglutamic acid (PGA) for functionalization to improve the oral uptake through calcium-sensing receptors and amino acid transporters present in intestinal epithelium.
30815757	3	80	theme	entrapment	874:883	arg1	efficiency					885:894	entrapment efficiency	874:894	entrapment efficiency	874:894	Morphological analysis demonstrated the formation of spherical NPs with particle size, zeta potential, and entrapment efficiency in the range of 210 nm ± 2.8 nm, 18.1 mV ± 0.14 mV, and 85.9% ± 0.28%, respectively.
31418592	0	0	theme	in	77:78	arg1	evaluation					98:107	in vitro and in vivo evaluation	77:107	Development and evaluation of rapidly dissolving buccal films of naftopidil: in vitro and in vivo evaluation.	0:108	Development and evaluation of rapidly dissolving buccal films of naftopidil: in vitro and in vivo evaluation.
31418592	5	1	used	utilized	647:654	arg2	film					642:645	Rapidly dissolving film	623:645	Rapidly dissolving film	623:645	Methods: Rapidly dissolving film utilized hydroxypropyl methylcellulose E5 and polyvinylpyrrolidone K30 as the main components.
31418592	7	2	dep	oleique	865:871	arg1	systems					933:939	self-microemulsifying drug delivery systems	897:939	self-microemulsifying drug delivery systems (SMEDDS)	897:948	A solution of naftopidil in plurol oleique, labrasol, and tween 80 self-microemulsifying drug delivery systems (SMEDDS) was also loaded.
31418592	7	2	dep	oleique	865:871	arg1	SMEDDS					942:947	SMEDDS	942:947	SMEDDS	942:947	A solution of naftopidil in plurol oleique, labrasol, and tween 80 self-microemulsifying drug delivery systems (SMEDDS) was also loaded.
31418592	7	3	theme	drug	919:922	arg1	systems					933:939	self-microemulsifying drug delivery systems	897:939	self-microemulsifying drug delivery systems (SMEDDS)	897:948	A solution of naftopidil in plurol oleique, labrasol, and tween 80 self-microemulsifying drug delivery systems (SMEDDS) was also loaded.
31418592	7	3	theme	drug	919:922	arg1	SMEDDS					942:947	SMEDDS	942:947	SMEDDS	942:947	A solution of naftopidil in plurol oleique, labrasol, and tween 80 self-microemulsifying drug delivery systems (SMEDDS) was also loaded.
31418592	4	4	theme	geriatric	594:602	arg1	patients					604:611	geriatric patients	594:611	geriatric patients	594:611	In addition, this film guarantees patient compliance and is suitable for geriatric patients.
31418592	6	5	with	co-grinding	784:794	arg1	acid					824:827	citric and/or tartaric acid	801:827	citric and/or tartaric acid	801:827	The drug was loaded in pure form or after co-grinding with citric and/or tartaric acid.
31418592	7	6	from	solution	832:839	arg1	labrasol					874:881	labrasol	874:881	labrasol	874:881	A solution of naftopidil in plurol oleique, labrasol, and tween 80 self-microemulsifying drug delivery systems (SMEDDS) was also loaded.
31418592	7	6	from	solution	832:839	arg1	tween					888:892	tween	888:892	tween	888:892	A solution of naftopidil in plurol oleique, labrasol, and tween 80 self-microemulsifying drug delivery systems (SMEDDS) was also loaded.
31418592	7	6	from	solution	832:839	arg1	oleique					865:871	oleique	865:871	oleique	865:871	A solution of naftopidil in plurol oleique, labrasol, and tween 80 self-microemulsifying drug delivery systems (SMEDDS) was also loaded.
31418592	8	7	theme	drug	991:994	arg1	interactions					971:982	The interactions	967:982	The interactions of the drug with the excipients	967:1014	The interactions of the drug with the excipients were monitored using thermal analysis, Fourier transform infrared spectroscopy, and X-ray diffraction.
31418592	2	8	theme	naftopidil	348:357	arg1	problem					337:343	the dissolution problem	321:343	the dissolution problem of naftopidil	321:357	Significance: Formulation of naftopidil in the form of rapidly dissolving buccal film can eliminate the dissolution problem of naftopidil and provide a greater chance for direct absorption into the systemic circulation bypassing the presystemic metabolism.
31418592	15	9	theme	dissolving	1783:1792	arg1	film					1801:1804	rapidly dissolving buccal film	1775:1804	rapidly dissolving buccal film	1775:1804	Conclusion: The study introduced rapidly dissolving buccal film for enhanced dissolution and bioavailability of naftopidil.
31418592	8	10	dep	Fourier	1055:1061	arg1	transform					1063:1071	transform	1063:1071	transform infrared spectroscopy	1063:1093	The interactions of the drug with the excipients were monitored using thermal analysis, Fourier transform infrared spectroscopy, and X-ray diffraction.
31418592	12	11	theme	SMEDDS	1495:1500	arg1	presence					1466:1473	the presence	1462:1473	the presence of organic acids or SMEDDS	1462:1500	The crystallinity of drug was abolished in the presence of organic acids or SMEDDS.
31418592	5	12	theme	polyvinylpyrrolidone	693:712	arg1	components					730:739	the main components	721:739	the main components	721:739	Methods: Rapidly dissolving film utilized hydroxypropyl methylcellulose E5 and polyvinylpyrrolidone K30 as the main components.
31418592	5	12	theme	polyvinylpyrrolidone	693:712	arg1	E5					686:687	hydroxypropyl methylcellulose E5	656:687	hydroxypropyl methylcellulose E5	656:687	Methods: Rapidly dissolving film utilized hydroxypropyl methylcellulose E5 and polyvinylpyrrolidone K30 as the main components.
31418592	5	12	theme	polyvinylpyrrolidone	693:712	arg1	K30					714:716	polyvinylpyrrolidone K30	693:716	polyvinylpyrrolidone K30	693:716	Methods: Rapidly dissolving film utilized hydroxypropyl methylcellulose E5 and polyvinylpyrrolidone K30 as the main components.
31418592	1	13	dep	Objectives	110:119	arg1	Objectives					110:119	Objectives	110:119	Objectives: Development and evaluation of rapidly dissolving film for intra-oral administration of naftopidil.	110:219	Objectives: Development and evaluation of rapidly dissolving film for intra-oral administration of naftopidil.
31418592	1	13	dep	Objectives	110:119	arg1	evaluation					138:147	evaluation	138:147	evaluation	138:147	Objectives: Development and evaluation of rapidly dissolving film for intra-oral administration of naftopidil.
31418592	1	13	dep	Objectives	110:119	arg1	Development					122:132	Development	122:132	Development	122:132	Objectives: Development and evaluation of rapidly dissolving film for intra-oral administration of naftopidil.
31418592	14	14	theme	administered	1674:1685	arg1	suspension					1687:1696	orally administered suspension	1667:1696	orally administered suspension	1667:1696	Those films enhanced the bioavailability of naftopidil compared to orally administered suspension with SMEDDS containing film being superior.
31418592	14	15	contain	containing	1710:1719	arg2	film					1721:1724	film	1721:1724	film	1721:1724	Those films enhanced the bioavailability of naftopidil compared to orally administered suspension with SMEDDS containing film being superior.
31418592	14	15	contain	containing	1710:1719	arg1	SMEDDS					1703:1708	SMEDDS	1703:1708	SMEDDS containing film	1703:1724	Those films enhanced the bioavailability of naftopidil compared to orally administered suspension with SMEDDS containing film being superior.
31418592	12	16	theme	drug	1440:1443	arg1	crystallinity					1423:1435	The crystallinity	1419:1435	The crystallinity of drug	1419:1443	The crystallinity of drug was abolished in the presence of organic acids or SMEDDS.
31418592	9	17	theme	buccal	1221:1226	arg1	administration					1228:1241	buccal administration	1221:1241	buccal administration to rabbit	1221:1251	Naftopidil dissolution was monitored and selected films were used to assess the bioavailability after buccal administration to rabbit.
31418592	0	18	dep	in	90:91	arg1	vivo					93:96	vivo	93:96	vivo	93:96	Development and evaluation of rapidly dissolving buccal films of naftopidil: in vitro and in vivo evaluation.
31418592	11	19	theme	new	1392:1394	arg1	structure					1408:1416	a new crystalline structure	1390:1416	a new crystalline structure	1390:1416	Results: Incorporation of naftopidil in the film developed a new crystalline structure.
31418592	5	20	theme	dissolving	631:640	arg1	film					642:645	Rapidly dissolving film	623:645	Rapidly dissolving film	623:645	Methods: Rapidly dissolving film utilized hydroxypropyl methylcellulose E5 and polyvinylpyrrolidone K30 as the main components.
31418592	15	21	theme	enhanced	1810:1817	arg1	dissolution					1819:1829	dissolution	1819:1829	dissolution	1819:1829	Conclusion: The study introduced rapidly dissolving buccal film for enhanced dissolution and bioavailability of naftopidil.
31418592	2	22	theme	direct	392:397	arg1	absorption					399:408	direct absorption	392:408	direct absorption into the systemic circulation bypassing the presystemic metabolism	392:475	Significance: Formulation of naftopidil in the form of rapidly dissolving buccal film can eliminate the dissolution problem of naftopidil and provide a greater chance for direct absorption into the systemic circulation bypassing the presystemic metabolism.
31418592	0	23	theme	in	90:91	arg1	evaluation					98:107	in vitro and in vivo evaluation	77:107	Development and evaluation of rapidly dissolving buccal films of naftopidil: in vitro and in vivo evaluation.	0:108	Development and evaluation of rapidly dissolving buccal films of naftopidil: in vitro and in vivo evaluation.
31418592	12	24	theme	organic	1478:1484	arg1	acids					1486:1490	organic acids	1478:1490	organic acids	1478:1490	The crystallinity of drug was abolished in the presence of organic acids or SMEDDS.
31418592	10	25	theme	Unprocessed	1254:1264	arg1	suspension					1271:1280	Unprocessed drug suspension	1254:1280	Unprocessed drug suspension	1254:1280	Unprocessed drug suspension was administered orally and used as a reference.
31418592	10	25	theme	Unprocessed	1254:1264	arg1	reference					1320:1328	a reference	1318:1328	a reference	1318:1328	Unprocessed drug suspension was administered orally and used as a reference.
31418592	2	26	from	Formulation	235:245	arg1	form					268:271	the form	264:271	the form of rapidly dissolving buccal film	264:305	Significance: Formulation of naftopidil in the form of rapidly dissolving buccal film can eliminate the dissolution problem of naftopidil and provide a greater chance for direct absorption into the systemic circulation bypassing the presystemic metabolism.
31418592	1	27	theme	dissolving	160:169	arg1	film					171:174	rapidly dissolving film	152:174	rapidly dissolving film for intra-oral administration of naftopidil	152:218	Objectives: Development and evaluation of rapidly dissolving film for intra-oral administration of naftopidil.
31418592	2	28	theme	presystemic	454:464	arg1	metabolism					466:475	the presystemic metabolism	450:475	the presystemic metabolism	450:475	Significance: Formulation of naftopidil in the form of rapidly dissolving buccal film can eliminate the dissolution problem of naftopidil and provide a greater chance for direct absorption into the systemic circulation bypassing the presystemic metabolism.
31418592	8	29	dep	transform	1063:1071	arg1	infrared					1073:1080	infrared	1073:1080	transform infrared spectroscopy	1063:1093	The interactions of the drug with the excipients were monitored using thermal analysis, Fourier transform infrared spectroscopy, and X-ray diffraction.
31418592	5	30	theme	hydroxypropyl	656:668	arg1	components					730:739	the main components	721:739	the main components	721:739	Methods: Rapidly dissolving film utilized hydroxypropyl methylcellulose E5 and polyvinylpyrrolidone K30 as the main components.
31418592	5	30	theme	hydroxypropyl	656:668	arg1	E5					686:687	hydroxypropyl methylcellulose E5	656:687	hydroxypropyl methylcellulose E5	656:687	Methods: Rapidly dissolving film utilized hydroxypropyl methylcellulose E5 and polyvinylpyrrolidone K30 as the main components.
31418592	5	30	theme	hydroxypropyl	656:668	arg1	K30					714:716	polyvinylpyrrolidone K30	693:716	polyvinylpyrrolidone K30	693:716	Methods: Rapidly dissolving film utilized hydroxypropyl methylcellulose E5 and polyvinylpyrrolidone K30 as the main components.
31418592	6	31	theme	tartaric	815:822	arg1	acid					824:827	citric and/or tartaric acid	801:827	citric and/or tartaric acid	801:827	The drug was loaded in pure form or after co-grinding with citric and/or tartaric acid.
31418592	0	32	dep	in	77:78	arg1	vitro					80:84	vitro	80:84	vitro	80:84	Development and evaluation of rapidly dissolving buccal films of naftopidil: in vitro and in vivo evaluation.
31418592	1	33	theme	film	171:174	arg1	Objectives					110:119	Objectives	110:119	Objectives: Development and evaluation of rapidly dissolving film for intra-oral administration of naftopidil.	110:219	Objectives: Development and evaluation of rapidly dissolving film for intra-oral administration of naftopidil.
31418592	1	33	theme	film	171:174	arg1	evaluation					138:147	evaluation	138:147	evaluation	138:147	Objectives: Development and evaluation of rapidly dissolving film for intra-oral administration of naftopidil.
31418592	1	33	theme	film	171:174	arg1	Development					122:132	Development	122:132	Development	122:132	Objectives: Development and evaluation of rapidly dissolving film for intra-oral administration of naftopidil.
31418592	5	34	theme	methylcellulose	670:684	arg1	components					730:739	the main components	721:739	the main components	721:739	Methods: Rapidly dissolving film utilized hydroxypropyl methylcellulose E5 and polyvinylpyrrolidone K30 as the main components.
31418592	5	34	theme	methylcellulose	670:684	arg1	E5					686:687	hydroxypropyl methylcellulose E5	656:687	hydroxypropyl methylcellulose E5	656:687	Methods: Rapidly dissolving film utilized hydroxypropyl methylcellulose E5 and polyvinylpyrrolidone K30 as the main components.
31418592	5	34	theme	methylcellulose	670:684	arg1	K30					714:716	polyvinylpyrrolidone K30	693:716	polyvinylpyrrolidone K30	693:716	Methods: Rapidly dissolving film utilized hydroxypropyl methylcellulose E5 and polyvinylpyrrolidone K30 as the main components.
31418592	12	35	theme	acids	1486:1490	arg1	presence					1466:1473	the presence	1462:1473	the presence of organic acids or SMEDDS	1462:1500	The crystallinity of drug was abolished in the presence of organic acids or SMEDDS.
31418592	9	36	theme	Naftopidil	1119:1128	arg1	dissolution					1130:1140	Naftopidil dissolution	1119:1140	Naftopidil dissolution	1119:1140	Naftopidil dissolution was monitored and selected films were used to assess the bioavailability after buccal administration to rabbit.
31418592	2	37	theme	greater	373:379	arg1	chance					381:386	a greater chance	371:386	a greater chance for direct absorption into the systemic circulation bypassing the presystemic metabolism	371:475	Significance: Formulation of naftopidil in the form of rapidly dissolving buccal film can eliminate the dissolution problem of naftopidil and provide a greater chance for direct absorption into the systemic circulation bypassing the presystemic metabolism.
31418592	11	38	from	Incorporation	1340:1352	arg1	film					1375:1378	the film	1371:1378	the film	1371:1378	Results: Incorporation of naftopidil in the film developed a new crystalline structure.
31418592	8	39	theme	X-ray	1100:1104	arg1	diffraction					1106:1116	X-ray diffraction	1100:1116	X-ray diffraction	1100:1116	The interactions of the drug with the excipients were monitored using thermal analysis, Fourier transform infrared spectroscopy, and X-ray diffraction.
31418592	6	40	theme	citric	801:806	arg1	acid					824:827	citric and/or tartaric acid	801:827	citric and/or tartaric acid	801:827	The drug was loaded in pure form or after co-grinding with citric and/or tartaric acid.
31418592	2	41	theme	film	302:305	arg1	form					268:271	the form	264:271	the form of rapidly dissolving buccal film	264:305	Significance: Formulation of naftopidil in the form of rapidly dissolving buccal film can eliminate the dissolution problem of naftopidil and provide a greater chance for direct absorption into the systemic circulation bypassing the presystemic metabolism.
31418592	2	42	theme	systemic	419:426	arg1	circulation					428:438	the systemic circulation	415:438	the systemic circulation bypassing the presystemic metabolism	415:475	Significance: Formulation of naftopidil in the form of rapidly dissolving buccal film can eliminate the dissolution problem of naftopidil and provide a greater chance for direct absorption into the systemic circulation bypassing the presystemic metabolism.
31418592	6	43	theme	pure	765:768	arg1	form					770:773	pure form	765:773	pure form	765:773	The drug was loaded in pure form or after co-grinding with citric and/or tartaric acid.
31418592	14	44	theme	naftopidil	1644:1653	arg1	bioavailability					1625:1639	the bioavailability	1621:1639	the bioavailability of naftopidil	1621:1653	Those films enhanced the bioavailability of naftopidil compared to orally administered suspension with SMEDDS containing film being superior.
31418592	2	45	theme	buccal	295:300	arg1	film					302:305	rapidly dissolving buccal film	276:305	rapidly dissolving buccal film	276:305	Significance: Formulation of naftopidil in the form of rapidly dissolving buccal film can eliminate the dissolution problem of naftopidil and provide a greater chance for direct absorption into the systemic circulation bypassing the presystemic metabolism.
31418592	7	46	theme	naftopidil	844:853	arg1	solution					832:839	A solution	830:839	A solution of naftopidil in plurol oleique, labrasol, and tween 80 self-microemulsifying drug delivery systems (SMEDDS)	830:948	A solution of naftopidil in plurol oleique, labrasol, and tween 80 self-microemulsifying drug delivery systems (SMEDDS) was also loaded.
31418592	0	47	theme	dissolving	38:47	arg1	films					56:60	rapidly dissolving buccal films	30:60	rapidly dissolving buccal films of naftopidil	30:74	Development and evaluation of rapidly dissolving buccal films of naftopidil: in vitro and in vivo evaluation.
31418592	15	48	theme	naftopidil	1854:1863	arg1	dissolution					1819:1829	dissolution	1819:1829	dissolution	1819:1829	Conclusion: The study introduced rapidly dissolving buccal film for enhanced dissolution and bioavailability of naftopidil.
31418592	15	48	theme	naftopidil	1854:1863	arg1	bioavailability					1835:1849	bioavailability	1835:1849	bioavailability	1835:1849	Conclusion: The study introduced rapidly dissolving buccal film for enhanced dissolution and bioavailability of naftopidil.
31418592	13	49	theme	dissolving	1515:1524	arg1	films					1526:1530	The rapidly dissolving films	1503:1530	The rapidly dissolving films	1503:1530	The rapidly dissolving films showed fast liberation of the drug irrespective to the composition.
31418592	8	50	with	interactions	971:982	arg1	excipients					1005:1014	the excipients	1001:1014	the excipients	1001:1014	The interactions of the drug with the excipients were monitored using thermal analysis, Fourier transform infrared spectroscopy, and X-ray diffraction.
31418592	13	51	theme	drug	1562:1565	arg1	liberation					1544:1553	fast liberation	1539:1553	fast liberation of the drug	1539:1565	The rapidly dissolving films showed fast liberation of the drug irrespective to the composition.
31418592	1	52	theme	naftopidil	209:218	arg1	administration					191:204	intra-oral administration	180:204	intra-oral administration of naftopidil	180:218	Objectives: Development and evaluation of rapidly dissolving film for intra-oral administration of naftopidil.
31418592	0	53	theme	films	56:60	arg1	evaluation					16:25	evaluation	16:25	evaluation	16:25	Development and evaluation of rapidly dissolving buccal films of naftopidil: in vitro and in vivo evaluation.
31418592	0	53	theme	films	56:60	arg1	Development					0:10	Development	0:10	Development	0:10	Development and evaluation of rapidly dissolving buccal films of naftopidil: in vitro and in vivo evaluation.
31418592	9	54	theme	selected	1160:1167	arg1	films					1169:1173	selected films	1160:1173	selected films	1160:1173	Naftopidil dissolution was monitored and selected films were used to assess the bioavailability after buccal administration to rabbit.
31418592	3	55	theme	oral	499:502	arg1	bioavailability					504:518	the oral bioavailability	495:518	the oral bioavailability	495:518	This can improve the oral bioavailability.
31418592	0	56	theme	buccal	49:54	arg1	films					56:60	rapidly dissolving buccal films	30:60	rapidly dissolving buccal films of naftopidil	30:74	Development and evaluation of rapidly dissolving buccal films of naftopidil: in vitro and in vivo evaluation.
31418592	4	57	theme	patient	555:561	arg1	compliance					563:572	patient compliance	555:572	patient compliance	555:572	In addition, this film guarantees patient compliance and is suitable for geriatric patients.
31418592	9	58	used	used	1180:1183	arg2	films					1169:1173	selected films	1160:1173	selected films	1160:1173	Naftopidil dissolution was monitored and selected films were used to assess the bioavailability after buccal administration to rabbit.
31418592	10	59	used	used	1310:1313	arg2	reference					1320:1328	a reference	1318:1328	a reference	1318:1328	Unprocessed drug suspension was administered orally and used as a reference.
31418592	10	59	used	used	1310:1313	arg2	suspension					1271:1280	Unprocessed drug suspension	1254:1280	Unprocessed drug suspension	1254:1280	Unprocessed drug suspension was administered orally and used as a reference.
31418592	5	60	theme	main	725:728	arg1	components					730:739	the main components	721:739	the main components	721:739	Methods: Rapidly dissolving film utilized hydroxypropyl methylcellulose E5 and polyvinylpyrrolidone K30 as the main components.
31418592	5	60	theme	main	725:728	arg1	E5					686:687	hydroxypropyl methylcellulose E5	656:687	hydroxypropyl methylcellulose E5	656:687	Methods: Rapidly dissolving film utilized hydroxypropyl methylcellulose E5 and polyvinylpyrrolidone K30 as the main components.
31418592	5	60	theme	main	725:728	arg1	K30					714:716	polyvinylpyrrolidone K30	693:716	polyvinylpyrrolidone K30	693:716	Methods: Rapidly dissolving film utilized hydroxypropyl methylcellulose E5 and polyvinylpyrrolidone K30 as the main components.
31418592	2	61	theme	dissolution	325:335	arg1	problem					337:343	the dissolution problem	321:343	the dissolution problem of naftopidil	321:357	Significance: Formulation of naftopidil in the form of rapidly dissolving buccal film can eliminate the dissolution problem of naftopidil and provide a greater chance for direct absorption into the systemic circulation bypassing the presystemic metabolism.
31418592	11	62	theme	crystalline	1396:1406	arg1	structure					1408:1416	a new crystalline structure	1390:1416	a new crystalline structure	1390:1416	Results: Incorporation of naftopidil in the film developed a new crystalline structure.
31418592	2	63	theme	dissolving	284:293	arg1	film					302:305	rapidly dissolving buccal film	276:305	rapidly dissolving buccal film	276:305	Significance: Formulation of naftopidil in the form of rapidly dissolving buccal film can eliminate the dissolution problem of naftopidil and provide a greater chance for direct absorption into the systemic circulation bypassing the presystemic metabolism.
31418592	7	64	theme	delivery	924:931	arg1	systems					933:939	self-microemulsifying drug delivery systems	897:939	self-microemulsifying drug delivery systems (SMEDDS)	897:948	A solution of naftopidil in plurol oleique, labrasol, and tween 80 self-microemulsifying drug delivery systems (SMEDDS) was also loaded.
31418592	7	64	theme	delivery	924:931	arg1	SMEDDS					942:947	SMEDDS	942:947	SMEDDS	942:947	A solution of naftopidil in plurol oleique, labrasol, and tween 80 self-microemulsifying drug delivery systems (SMEDDS) was also loaded.
31418592	13	65	theme	fast	1539:1542	arg1	liberation					1544:1553	fast liberation	1539:1553	fast liberation of the drug	1539:1565	The rapidly dissolving films showed fast liberation of the drug irrespective to the composition.
31418592	0	66	theme	naftopidil	65:74	arg1	films					56:60	rapidly dissolving buccal films	30:60	rapidly dissolving buccal films of naftopidil	30:74	Development and evaluation of rapidly dissolving buccal films of naftopidil: in vitro and in vivo evaluation.
31418592	15	67	theme	buccal	1794:1799	arg1	film					1801:1804	rapidly dissolving buccal film	1775:1804	rapidly dissolving buccal film	1775:1804	Conclusion: The study introduced rapidly dissolving buccal film for enhanced dissolution and bioavailability of naftopidil.
31418592	2	68	theme	naftopidil	250:259	arg1	Formulation					235:245	Formulation	235:245	Formulation of naftopidil in the form of rapidly dissolving buccal film	235:305	Significance: Formulation of naftopidil in the form of rapidly dissolving buccal film can eliminate the dissolution problem of naftopidil and provide a greater chance for direct absorption into the systemic circulation bypassing the presystemic metabolism.
31418592	1	69	theme	intra-oral	180:189	arg1	administration					191:204	intra-oral administration	180:204	intra-oral administration of naftopidil	180:218	Objectives: Development and evaluation of rapidly dissolving film for intra-oral administration of naftopidil.
31418592	10	70	theme	drug	1266:1269	arg1	suspension					1271:1280	Unprocessed drug suspension	1254:1280	Unprocessed drug suspension	1254:1280	Unprocessed drug suspension was administered orally and used as a reference.
31418592	10	70	theme	drug	1266:1269	arg1	reference					1320:1328	a reference	1318:1328	a reference	1318:1328	Unprocessed drug suspension was administered orally and used as a reference.
31418592	8	71	theme	thermal	1037:1043	arg1	analysis					1045:1052	thermal analysis	1037:1052	thermal analysis	1037:1052	The interactions of the drug with the excipients were monitored using thermal analysis, Fourier transform infrared spectroscopy, and X-ray diffraction.
31418592	11	72	theme	naftopidil	1357:1366	arg1	Incorporation					1340:1352	Incorporation	1340:1352	Incorporation of naftopidil in the film	1340:1378	Results: Incorporation of naftopidil in the film developed a new crystalline structure.
31418592	0	73	dep	Development	0:10	arg1	evaluation					98:107	in vitro and in vivo evaluation	77:107	Development and evaluation of rapidly dissolving buccal films of naftopidil: in vitro and in vivo evaluation.	0:108	Development and evaluation of rapidly dissolving buccal films of naftopidil: in vitro and in vivo evaluation.
31418592	7	74	theme	self-microemulsifying	897:917	arg1	systems					933:939	self-microemulsifying drug delivery systems	897:939	self-microemulsifying drug delivery systems (SMEDDS)	897:948	A solution of naftopidil in plurol oleique, labrasol, and tween 80 self-microemulsifying drug delivery systems (SMEDDS) was also loaded.
31418592	7	74	theme	self-microemulsifying	897:917	arg1	SMEDDS					942:947	SMEDDS	942:947	SMEDDS	942:947	A solution of naftopidil in plurol oleique, labrasol, and tween 80 self-microemulsifying drug delivery systems (SMEDDS) was also loaded.
30926525	3	0	theme	molar	519:523	arg1	ratio					525:529	The specific molar ratio	506:529	The specific molar ratio of the three components	506:553	The specific molar ratio of the three components was established after studying the mixing properties of the lipid monolayers of the lipid components formed at the air-water interface.
30926525	1	1	theme	molar	264:268	arg1	composition					270:280	a thermodynamically optimized molar composition	234:280	a thermodynamically optimized molar composition formed by the main components of the stratum corneum matrix	234:340	The fundamental objective pursued in this work is to investigate how liposomes formed with a thermodynamically optimized molar composition formed by the main components of the stratum corneum matrix behave on the human skin surface when used as drug delivery systems.
30926525	5	2	theme	atomic	956:961	arg1	microscopy					969:978	atomic force microscopy	956:978	atomic force microscopy	956:978	Optimized liposomes, with and without drugs, were applied onto human skin and the structures formed evaluated using atomic force microscopy.
30926525	7	3	from	extension	1246:1254	arg1	suspension					1272:1281	suspension	1272:1281	suspension	1272:1281	In the present work we were able to observe the deposition and extension of liposomes in suspension onto human skin demonstrating the potential of liposomes without a secondary vehicle for releasing drugs in transdermal applications.
30926525	0	4	theme	delivery	106:113	arg1	systems					115:121	drug delivery systems	101:121	drug delivery systems through human skin	101:140	Planar lipid bilayers formed from thermodynamically-optimized liposomes as new featured carriers for drug delivery systems through human skin.
30926525	1	5	theme	human	356:360	arg1	surface					367:373	the human skin surface	352:373	the human skin surface	352:373	The fundamental objective pursued in this work is to investigate how liposomes formed with a thermodynamically optimized molar composition formed by the main components of the stratum corneum matrix behave on the human skin surface when used as drug delivery systems.
30926525	6	6	theme	drugs	1039:1043	arg1	permeation					1021:1030	the permeation	1017:1030	the permeation of the drugs encapsulated	1017:1056	Since penetration enhancers improve the permeation of the drugs encapsulated, we also examined the effects of Tween® 80 on the physical properties of the liposomes and on their extensibility over skin.
30926525	0	7	theme	drug	101:104	arg1	systems					115:121	drug delivery systems	101:121	drug delivery systems through human skin	101:140	Planar lipid bilayers formed from thermodynamically-optimized liposomes as new featured carriers for drug delivery systems through human skin.
30926525	1	8	theme	skin	362:365	arg1	surface					367:373	the human skin surface	352:373	the human skin surface	352:373	The fundamental objective pursued in this work is to investigate how liposomes formed with a thermodynamically optimized molar composition formed by the main components of the stratum corneum matrix behave on the human skin surface when used as drug delivery systems.
30926525	4	9	theme	light	792:796	arg1	scattering					798:807	quasi-elastic light scattering	778:807	quasi-elastic light scattering	778:807	Liposomes loaded and unloaded with ibuprofen and hyaluronic acid were characterized by quasi-elastic light scattering and fluorescence polarization.
30926525	7	10	from	work	1198:1201	arg1	able					1211:1214	able	1211:1214	able	1211:1214	In the present work we were able to observe the deposition and extension of liposomes in suspension onto human skin demonstrating the potential of liposomes without a secondary vehicle for releasing drugs in transdermal applications.
30926525	4	11	theme	fluorescence	813:824	arg1	polarization					826:837	fluorescence polarization	813:837	fluorescence polarization	813:837	Liposomes loaded and unloaded with ibuprofen and hyaluronic acid were characterized by quasi-elastic light scattering and fluorescence polarization.
30926525	7	12	from	drugs	1382:1386	arg1	applications					1403:1414	transdermal applications	1391:1414	transdermal applications	1391:1414	In the present work we were able to observe the deposition and extension of liposomes in suspension onto human skin demonstrating the potential of liposomes without a secondary vehicle for releasing drugs in transdermal applications.
30926525	3	13	theme	components	544:553	arg1	ratio					525:529	The specific molar ratio	506:529	The specific molar ratio of the three components	506:553	The specific molar ratio of the three components was established after studying the mixing properties of the lipid monolayers of the lipid components formed at the air-water interface.
30926525	5	14	theme	human	903:907	arg1	skin					909:912	human skin	903:912	human skin	903:912	Optimized liposomes, with and without drugs, were applied onto human skin and the structures formed evaluated using atomic force microscopy.
30926525	0	15	theme	lipid	7:11	arg1	bilayers					13:20	Planar lipid bilayers	0:20	Planar lipid bilayers	0:20	Planar lipid bilayers formed from thermodynamically-optimized liposomes as new featured carriers for drug delivery systems through human skin.
30926525	6	16	from	effects	1080:1086	arg1	extensibility					1158:1170	their extensibility	1152:1170	their extensibility over skin	1152:1180	Since penetration enhancers improve the permeation of the drugs encapsulated, we also examined the effects of Tween® 80 on the physical properties of the liposomes and on their extensibility over skin.
30926525	6	16	from	effects	1080:1086	arg1	properties					1117:1126	the physical properties	1104:1126	the physical properties of the liposomes	1104:1143	Since penetration enhancers improve the permeation of the drugs encapsulated, we also examined the effects of Tween® 80 on the physical properties of the liposomes and on their extensibility over skin.
30926525	5	17	dep	formed	933:938	arg1	evaluated					940:948	evaluated	940:948	formed evaluated using atomic force microscopy	933:978	Optimized liposomes, with and without drugs, were applied onto human skin and the structures formed evaluated using atomic force microscopy.
30926525	0	18	theme	Planar	0:5	arg1	bilayers					13:20	Planar lipid bilayers	0:20	Planar lipid bilayers	0:20	Planar lipid bilayers formed from thermodynamically-optimized liposomes as new featured carriers for drug delivery systems through human skin.
30926525	3	19	theme	mixing	590:595	arg1	properties					597:606	the mixing properties	586:606	the mixing properties of the lipid monolayers of the lipid components formed at the air-water interface	586:688	The specific molar ratio of the three components was established after studying the mixing properties of the lipid monolayers of the lipid components formed at the air-water interface.
30926525	7	20	theme	transdermal	1391:1401	arg1	applications					1403:1414	transdermal applications	1391:1414	transdermal applications	1391:1414	In the present work we were able to observe the deposition and extension of liposomes in suspension onto human skin demonstrating the potential of liposomes without a secondary vehicle for releasing drugs in transdermal applications.
30926525	7	21	theme	secondary	1350:1358	arg1	vehicle					1360:1366	a secondary vehicle	1348:1366	a secondary vehicle for releasing drugs in transdermal applications	1348:1414	In the present work we were able to observe the deposition and extension of liposomes in suspension onto human skin demonstrating the potential of liposomes without a secondary vehicle for releasing drugs in transdermal applications.
30926525	7	22	theme	present	1190:1196	arg1	work					1198:1201	the present work	1186:1201	the present work	1186:1201	In the present work we were able to observe the deposition and extension of liposomes in suspension onto human skin demonstrating the potential of liposomes without a secondary vehicle for releasing drugs in transdermal applications.
30926525	1	23	theme	main	296:299	arg1	components					301:310	the main components	292:310	the main components of the stratum corneum matrix	292:340	The fundamental objective pursued in this work is to investigate how liposomes formed with a thermodynamically optimized molar composition formed by the main components of the stratum corneum matrix behave on the human skin surface when used as drug delivery systems.
30926525	7	24	dep	deposition	1231:1240	arg1	the					1227:1229	the	1227:1229	the	1227:1229	In the present work we were able to observe the deposition and extension of liposomes in suspension onto human skin demonstrating the potential of liposomes without a secondary vehicle for releasing drugs in transdermal applications.
30926525	4	25	theme	quasi-elastic	778:790	arg1	scattering					798:807	quasi-elastic light scattering	778:807	quasi-elastic light scattering	778:807	Liposomes loaded and unloaded with ibuprofen and hyaluronic acid were characterized by quasi-elastic light scattering and fluorescence polarization.
30926525	7	26	theme	liposomes	1330:1338	arg1	potential					1317:1325	the potential	1313:1325	the potential of liposomes	1313:1338	In the present work we were able to observe the deposition and extension of liposomes in suspension onto human skin demonstrating the potential of liposomes without a secondary vehicle for releasing drugs in transdermal applications.
30926525	0	27	theme	human	131:135	arg1	skin					137:140	human skin	131:140	human skin	131:140	Planar lipid bilayers formed from thermodynamically-optimized liposomes as new featured carriers for drug delivery systems through human skin.
30926525	7	28	from	able	1211:1214	arg1	work					1198:1201	the present work	1186:1201	the present work	1186:1201	In the present work we were able to observe the deposition and extension of liposomes in suspension onto human skin demonstrating the potential of liposomes without a secondary vehicle for releasing drugs in transdermal applications.
30926525	7	29	theme	liposomes	1259:1267	arg1	extension					1246:1254	extension	1246:1254	extension	1246:1254	In the present work we were able to observe the deposition and extension of liposomes in suspension onto human skin demonstrating the potential of liposomes without a secondary vehicle for releasing drugs in transdermal applications.
30926525	7	29	theme	liposomes	1259:1267	arg1	deposition					1231:1240	deposition	1231:1240	deposition	1231:1240	In the present work we were able to observe the deposition and extension of liposomes in suspension onto human skin demonstrating the potential of liposomes without a secondary vehicle for releasing drugs in transdermal applications.
30926525	6	30	theme	Tween®	1091:1096	arg1	effects					1080:1086	the effects	1076:1086	the effects of Tween® 80 on the physical properties of the liposomes and on their extensibility over skin	1076:1180	Since penetration enhancers improve the permeation of the drugs encapsulated, we also examined the effects of Tween® 80 on the physical properties of the liposomes and on their extensibility over skin.
30926525	0	31	theme	thermodynamically-optimized	34:60	arg1	liposomes					62:70	thermodynamically-optimized liposomes	34:70	thermodynamically-optimized liposomes as new featured carriers for drug delivery systems through human skin	34:140	Planar lipid bilayers formed from thermodynamically-optimized liposomes as new featured carriers for drug delivery systems through human skin.
30926525	3	32	theme	specific	510:517	arg1	ratio					525:529	The specific molar ratio	506:529	The specific molar ratio of the three components	506:553	The specific molar ratio of the three components was established after studying the mixing properties of the lipid monolayers of the lipid components formed at the air-water interface.
30926525	1	33	theme	drug	388:391	arg1	systems					402:408	drug delivery systems	388:408	drug delivery systems	388:408	The fundamental objective pursued in this work is to investigate how liposomes formed with a thermodynamically optimized molar composition formed by the main components of the stratum corneum matrix behave on the human skin surface when used as drug delivery systems.
30926525	1	34	theme	delivery	393:400	arg1	systems					402:408	drug delivery systems	388:408	drug delivery systems	388:408	The fundamental objective pursued in this work is to investigate how liposomes formed with a thermodynamically optimized molar composition formed by the main components of the stratum corneum matrix behave on the human skin surface when used as drug delivery systems.
30926525	3	35	theme	lipid	615:619	arg1	monolayers					621:630	the lipid monolayers	611:630	the lipid monolayers of the lipid components formed at the air-water interface	611:688	The specific molar ratio of the three components was established after studying the mixing properties of the lipid monolayers of the lipid components formed at the air-water interface.
30926525	5	36	theme	Optimized	840:848	arg1	liposomes					850:858	Optimized liposomes	840:858	Optimized liposomes	840:858	Optimized liposomes, with and without drugs, were applied onto human skin and the structures formed evaluated using atomic force microscopy.
30926525	1	37	theme	stratum	319:325	arg1	corneum					327:333	stratum corneum	319:333	the stratum corneum matrix	315:340	The fundamental objective pursued in this work is to investigate how liposomes formed with a thermodynamically optimized molar composition formed by the main components of the stratum corneum matrix behave on the human skin surface when used as drug delivery systems.
30926525	1	38	theme	corneum	327:333	arg1	matrix					335:340	the stratum corneum matrix	315:340	the stratum corneum matrix	315:340	The fundamental objective pursued in this work is to investigate how liposomes formed with a thermodynamically optimized molar composition formed by the main components of the stratum corneum matrix behave on the human skin surface when used as drug delivery systems.
30926525	7	39	from	deposition	1231:1240	arg1	suspension					1272:1281	suspension	1272:1281	suspension	1272:1281	In the present work we were able to observe the deposition and extension of liposomes in suspension onto human skin demonstrating the potential of liposomes without a secondary vehicle for releasing drugs in transdermal applications.
30926525	3	40	theme	components	645:654	arg1	monolayers					621:630	the lipid monolayers	611:630	the lipid monolayers of the lipid components formed at the air-water interface	611:688	The specific molar ratio of the three components was established after studying the mixing properties of the lipid monolayers of the lipid components formed at the air-water interface.
30926525	1	41	theme	matrix	335:340	arg1	components					301:310	the main components	292:310	the main components of the stratum corneum matrix	292:340	The fundamental objective pursued in this work is to investigate how liposomes formed with a thermodynamically optimized molar composition formed by the main components of the stratum corneum matrix behave on the human skin surface when used as drug delivery systems.
30926525	4	42	dep	Liposomes	691:699	arg1	unloaded					712:719	unloaded	712:719	unloaded with ibuprofen and hyaluronic acid	712:754	Liposomes loaded and unloaded with ibuprofen and hyaluronic acid were characterized by quasi-elastic light scattering and fluorescence polarization.
30926525	4	42	dep	Liposomes	691:699	arg1	loaded					701:706	loaded	701:706	loaded	701:706	Liposomes loaded and unloaded with ibuprofen and hyaluronic acid were characterized by quasi-elastic light scattering and fluorescence polarization.
30926525	5	43	theme	force	963:967	arg1	microscopy					969:978	atomic force microscopy	956:978	atomic force microscopy	956:978	Optimized liposomes, with and without drugs, were applied onto human skin and the structures formed evaluated using atomic force microscopy.
30926525	3	44	theme	air-water	670:678	arg1	interface					680:688	the air-water interface	666:688	the air-water interface	666:688	The specific molar ratio of the three components was established after studying the mixing properties of the lipid monolayers of the lipid components formed at the air-water interface.
30926525	3	45	theme	lipid	639:643	arg1	components					645:654	the lipid components	635:654	the lipid components formed at the air-water interface	635:688	The specific molar ratio of the three components was established after studying the mixing properties of the lipid monolayers of the lipid components formed at the air-water interface.
30926525	4	46	theme	hyaluronic	740:749	arg1	acid					751:754	hyaluronic acid	740:754	hyaluronic acid	740:754	Liposomes loaded and unloaded with ibuprofen and hyaluronic acid were characterized by quasi-elastic light scattering and fluorescence polarization.
30926525	7	47	theme	human	1288:1292	arg1	skin					1294:1297	human skin	1288:1297	human skin	1288:1297	In the present work we were able to observe the deposition and extension of liposomes in suspension onto human skin demonstrating the potential of liposomes without a secondary vehicle for releasing drugs in transdermal applications.
30926525	0	48	theme	featured	79:86	arg1	carriers					88:95	new featured carriers	75:95	new featured carriers for drug delivery systems through human skin	75:140	Planar lipid bilayers formed from thermodynamically-optimized liposomes as new featured carriers for drug delivery systems through human skin.
30926525	3	49	theme	monolayers	621:630	arg1	properties					597:606	the mixing properties	586:606	the mixing properties of the lipid monolayers of the lipid components formed at the air-water interface	586:688	The specific molar ratio of the three components was established after studying the mixing properties of the lipid monolayers of the lipid components formed at the air-water interface.
30926525	1	50	theme	fundamental	147:157	arg1	objective					159:167	The fundamental objective	143:167	The fundamental objective pursued in this work	143:188	The fundamental objective pursued in this work is to investigate how liposomes formed with a thermodynamically optimized molar composition formed by the main components of the stratum corneum matrix behave on the human skin surface when used as drug delivery systems.
30926525	6	51	theme	physical	1108:1115	arg1	properties					1117:1126	the physical properties	1104:1126	the physical properties of the liposomes	1104:1143	Since penetration enhancers improve the permeation of the drugs encapsulated, we also examined the effects of Tween® 80 on the physical properties of the liposomes and on their extensibility over skin.
30926525	0	52	theme	new	75:77	arg1	carriers					88:95	new featured carriers	75:95	new featured carriers for drug delivery systems through human skin	75:140	Planar lipid bilayers formed from thermodynamically-optimized liposomes as new featured carriers for drug delivery systems through human skin.
30926525	6	53	theme	liposomes	1135:1143	arg1	properties					1117:1126	the physical properties	1104:1126	the physical properties of the liposomes	1104:1143	Since penetration enhancers improve the permeation of the drugs encapsulated, we also examined the effects of Tween® 80 on the physical properties of the liposomes and on their extensibility over skin.
30926525	6	54	theme	penetration	987:997	arg1	enhancers					999:1007	penetration enhancers	987:1007	penetration enhancers	987:1007	Since penetration enhancers improve the permeation of the drugs encapsulated, we also examined the effects of Tween® 80 on the physical properties of the liposomes and on their extensibility over skin.
30926525	1	55	theme	optimized	254:262	arg1	composition					270:280	a thermodynamically optimized molar composition	234:280	a thermodynamically optimized molar composition formed by the main components of the stratum corneum matrix	234:340	The fundamental objective pursued in this work is to investigate how liposomes formed with a thermodynamically optimized molar composition formed by the main components of the stratum corneum matrix behave on the human skin surface when used as drug delivery systems.
30767541	10	0	from	microbiota	1364:1373	arg1	pronounced					1329:1338	pronounced	1329:1338	pronounced	1329:1338	In summary, functional changes in the distal colon caused by DSS treatment were more pronounced in the mucus-associated microbiota than in the microbiota present in the distal colon content.
30767541	10	1	theme	distal	1282:1287	arg1	colon					1289:1293	the distal colon	1278:1293	the distal colon caused by DSS treatment	1278:1317	In summary, functional changes in the distal colon caused by DSS treatment were more pronounced in the mucus-associated microbiota than in the microbiota present in the distal colon content.
30767541	1	2	theme	colitis	212:218	arg1	model					220:224	the acute dextran sulfate sodium (DSS)-induced colitis model	165:224	the acute dextran sulfate sodium (DSS)-induced colitis model	165:224	Using the acute dextran sulfate sodium (DSS)-induced colitis model, studies have demonstrated that intestinal inflammation is accompanied by major changes in the composition of the intestinal microbiota.
30767541	0	3	theme	Colitis	150:156	arg1	Model					104:108	a Refined Model	94:108	a Refined Model of Dextran Sulfate Sodium (DSS)-Induced Colitis	94:156	Disease Development Is Accompanied by Changes in Bacterial Protein Abundance and Functions in a Refined Model of Dextran Sulfate Sodium (DSS)-Induced Colitis.
30767541	3	4	theme	detrimental	606:616	arg1	loss					625:628	detrimental weight loss	606:628	detrimental weight loss usually observed in DSS models	606:659	We used a refined model of chronic DSS-induced colitis that reflects typical symptoms of the human disease without detrimental weight loss usually observed in DSS models.
30767541	1	5	theme	microbiota	351:360	arg1	composition					321:331	the composition	317:331	the composition of the intestinal microbiota	317:360	Using the acute dextran sulfate sodium (DSS)-induced colitis model, studies have demonstrated that intestinal inflammation is accompanied by major changes in the composition of the intestinal microbiota.
30767541	4	6	theme	diseased	737:744	arg1	cohorts					752:758	healthy and diseased mouse cohorts	725:758	healthy and diseased mouse cohorts ( n = 12)	725:768	We sampled cecum and colon content as well as colon mucus from healthy and diseased mouse cohorts ( n = 12) and applied 16S rRNA gene sequencing and metaproteomics.
30767541	4	6	theme	diseased	737:744	arg1	n					762:762	n = 12	762:767	n = 12	762:767	We sampled cecum and colon content as well as colon mucus from healthy and diseased mouse cohorts ( n = 12) and applied 16S rRNA gene sequencing and metaproteomics.
30767541	3	7	theme	weight	618:623	arg1	loss					625:628	detrimental weight loss	606:628	detrimental weight loss usually observed in DSS models	606:659	We used a refined model of chronic DSS-induced colitis that reflects typical symptoms of the human disease without detrimental weight loss usually observed in DSS models.
30767541	3	8	theme	refined	501:507	arg1	model					509:513	a refined model	499:513	a refined model of chronic DSS-induced colitis that reflects typical symptoms of the human disease without detrimental weight loss usually observed in DSS models	499:659	We used a refined model of chronic DSS-induced colitis that reflects typical symptoms of the human disease without detrimental weight loss usually observed in DSS models.
30767541	4	9	dep	cecum	673:677	arg1	content					689:695	content	689:695	content	689:695	We sampled cecum and colon content as well as colon mucus from healthy and diseased mouse cohorts ( n = 12) and applied 16S rRNA gene sequencing and metaproteomics.
30767541	10	10	theme	DSS	1305:1307	arg1	treatment					1309:1317	DSS treatment	1305:1317	DSS treatment	1305:1317	In summary, functional changes in the distal colon caused by DSS treatment were more pronounced in the mucus-associated microbiota than in the microbiota present in the distal colon content.
30767541	10	11	theme	mucus-associated	1347:1362	arg1	microbiota					1364:1373	the mucus-associated microbiota	1343:1373	the mucus-associated microbiota	1343:1373	In summary, functional changes in the distal colon caused by DSS treatment were more pronounced in the mucus-associated microbiota than in the microbiota present in the distal colon content.
30767541	10	12	from	present	1398:1404	arg1	colon					1420:1424	the distal colon	1409:1424	the distal colon	1409:1424	In summary, functional changes in the distal colon caused by DSS treatment were more pronounced in the mucus-associated microbiota than in the microbiota present in the distal colon content.
30767541	8	13	theme	sulfate-reducing	1072:1087	arg1	Desulfovibrio					1055:1067	Desulfovibrio	1055:1067	Desulfovibrio	1055:1067	The abundance of Desulfovibrio, a sulfate-reducing bacterium, was positively associated with the carbon metabolism.
30767541	8	13	theme	sulfate-reducing	1072:1087	arg1	bacterium					1089:1097	a sulfate-reducing bacterium	1070:1097	a sulfate-reducing bacterium	1070:1097	The abundance of Desulfovibrio, a sulfate-reducing bacterium, was positively associated with the carbon metabolism.
30767541	10	14	theme	distal	1413:1418	arg1	colon					1420:1424	the distal colon	1409:1424	the distal colon	1409:1424	In summary, functional changes in the distal colon caused by DSS treatment were more pronounced in the mucus-associated microbiota than in the microbiota present in the distal colon content.
30767541	10	15	theme	functional	1256:1265	arg1	changes					1267:1273	functional changes	1256:1273	functional changes in the distal colon caused by DSS treatment	1256:1317	In summary, functional changes in the distal colon caused by DSS treatment were more pronounced in the mucus-associated microbiota than in the microbiota present in the distal colon content.
30767541	0	16	theme	Refined	96:102	arg1	Model					104:108	a Refined Model	94:108	a Refined Model of Dextran Sulfate Sodium (DSS)-Induced Colitis	94:156	Disease Development Is Accompanied by Changes in Bacterial Protein Abundance and Functions in a Refined Model of Dextran Sulfate Sodium (DSS)-Induced Colitis.
30767541	0	17	from	Changes	38:44	arg1	Functions					81:89	Functions	81:89	Functions	81:89	Disease Development Is Accompanied by Changes in Bacterial Protein Abundance and Functions in a Refined Model of Dextran Sulfate Sodium (DSS)-Induced Colitis.
30767541	0	17	from	Changes	38:44	arg1	Abundance					67:75	Bacterial Protein Abundance	49:75	Bacterial Protein Abundance	49:75	Disease Development Is Accompanied by Changes in Bacterial Protein Abundance and Functions in a Refined Model of Dextran Sulfate Sodium (DSS)-Induced Colitis.
30767541	10	18	theme	present	1398:1404	arg1	content					1426:1432	the microbiota present in the distal colon content	1383:1432	the microbiota present in the distal colon content	1383:1432	In summary, functional changes in the distal colon caused by DSS treatment were more pronounced in the mucus-associated microbiota than in the microbiota present in the distal colon content.
30767541	4	19	theme	healthy	725:731	arg1	cohorts					752:758	healthy and diseased mouse cohorts	725:758	healthy and diseased mouse cohorts ( n = 12)	725:768	We sampled cecum and colon content as well as colon mucus from healthy and diseased mouse cohorts ( n = 12) and applied 16S rRNA gene sequencing and metaproteomics.
30767541	4	19	theme	healthy	725:731	arg1	n					762:762	n = 12	762:767	n = 12	762:767	We sampled cecum and colon content as well as colon mucus from healthy and diseased mouse cohorts ( n = 12) and applied 16S rRNA gene sequencing and metaproteomics.
30767541	3	20	used	used	494:497	arg2	We					491:492	We	491:492	We	491:492	We used a refined model of chronic DSS-induced colitis that reflects typical symptoms of the human disease without detrimental weight loss usually observed in DSS models.
30767541	8	21	theme	carbon	1135:1140	arg1	metabolism					1142:1151	the carbon metabolism	1131:1151	the carbon metabolism	1131:1151	The abundance of Desulfovibrio, a sulfate-reducing bacterium, was positively associated with the carbon metabolism.
30767541	1	22	theme	intestinal	340:349	arg1	microbiota					351:360	the intestinal microbiota	336:360	the intestinal microbiota	336:360	Using the acute dextran sulfate sodium (DSS)-induced colitis model, studies have demonstrated that intestinal inflammation is accompanied by major changes in the composition of the intestinal microbiota.
30767541	4	23	theme	rRNA	786:789	arg1	sequencing					796:805	16S rRNA gene sequencing	782:805	16S rRNA gene sequencing	782:805	We sampled cecum and colon content as well as colon mucus from healthy and diseased mouse cohorts ( n = 12) and applied 16S rRNA gene sequencing and metaproteomics.
30767541	10	24	theme	microbiota	1387:1396	arg1	content					1426:1432	the microbiota present in the distal colon content	1383:1432	the microbiota present in the distal colon content	1383:1432	In summary, functional changes in the distal colon caused by DSS treatment were more pronounced in the mucus-associated microbiota than in the microbiota present in the distal colon content.
30767541	3	25	theme	DSS-induced	526:536	arg1	colitis					538:544	chronic DSS-induced colitis	518:544	chronic DSS-induced colitis	518:544	We used a refined model of chronic DSS-induced colitis that reflects typical symptoms of the human disease without detrimental weight loss usually observed in DSS models.
30767541	10	26	attach	present	1398:1404	arg1	colon					1420:1424	the distal colon	1409:1424	the distal colon	1409:1424	In summary, functional changes in the distal colon caused by DSS treatment were more pronounced in the mucus-associated microbiota than in the microbiota present in the distal colon content.
30767541	10	26	attach	present	1398:1404	arg2	content					1426:1432	the microbiota present in the distal colon content	1383:1432	the microbiota present in the distal colon content	1383:1432	In summary, functional changes in the distal colon caused by DSS treatment were more pronounced in the mucus-associated microbiota than in the microbiota present in the distal colon content.
30767541	0	27	theme	Disease	0:6	arg1	Development					8:18	Disease Development	0:18	Disease Development	0:18	Disease Development Is Accompanied by Changes in Bacterial Protein Abundance and Functions in a Refined Model of Dextran Sulfate Sodium (DSS)-Induced Colitis.
30767541	4	28	theme	16S	782:784	arg1	sequencing					796:805	16S rRNA gene sequencing	782:805	16S rRNA gene sequencing	782:805	We sampled cecum and colon content as well as colon mucus from healthy and diseased mouse cohorts ( n = 12) and applied 16S rRNA gene sequencing and metaproteomics.
30767541	3	29	theme	colitis	538:544	arg1	model					509:513	a refined model	499:513	a refined model of chronic DSS-induced colitis that reflects typical symptoms of the human disease without detrimental weight loss usually observed in DSS models	499:659	We used a refined model of chronic DSS-induced colitis that reflects typical symptoms of the human disease without detrimental weight loss usually observed in DSS models.
30767541	4	30	theme	gene	791:794	arg1	sequencing					796:805	16S rRNA gene sequencing	782:805	16S rRNA gene sequencing	782:805	We sampled cecum and colon content as well as colon mucus from healthy and diseased mouse cohorts ( n = 12) and applied 16S rRNA gene sequencing and metaproteomics.
30767541	3	31	located	observed	638:645	arg1	models					654:659	DSS models	650:659	DSS models	650:659	We used a refined model of chronic DSS-induced colitis that reflects typical symptoms of the human disease without detrimental weight loss usually observed in DSS models.
30767541	3	31	located	observed	638:645	arg2	loss					625:628	detrimental weight loss	606:628	detrimental weight loss usually observed in DSS models	606:659	We used a refined model of chronic DSS-induced colitis that reflects typical symptoms of the human disease without detrimental weight loss usually observed in DSS models.
30767541	7	32	theme	content	1019:1025	arg1	importance					979:988	the importance	975:988	the importance of separately sampling lumen content and mucus	975:1035	Functional differences were observed between sample types demonstrating the importance of separately sampling lumen content and mucus.
30767541	4	33	theme	colon	708:712	arg1	mucus					714:718	colon mucus	708:718	cecum and colon content as well as colon mucus	673:718	We sampled cecum and colon content as well as colon mucus from healthy and diseased mouse cohorts ( n = 12) and applied 16S rRNA gene sequencing and metaproteomics.
30767541	4	34	theme	=	764:764	arg1	cohorts					752:758	healthy and diseased mouse cohorts	725:758	healthy and diseased mouse cohorts ( n = 12)	725:768	We sampled cecum and colon content as well as colon mucus from healthy and diseased mouse cohorts ( n = 12) and applied 16S rRNA gene sequencing and metaproteomics.
30767541	4	34	theme	=	764:764	arg1	n					762:762	n = 12	762:767	n = 12	762:767	We sampled cecum and colon content as well as colon mucus from healthy and diseased mouse cohorts ( n = 12) and applied 16S rRNA gene sequencing and metaproteomics.
30767541	2	35	theme	microbial	394:402	arg1	changes					404:410	the microbial changes	390:410	the microbial changes	390:410	Only little is known about the microbial changes and more importantly their functional impact in the chronic DSS colitis model.
30767541	10	36	from	colon	1420:1424	arg1	present					1398:1404	present	1398:1404	present	1398:1404	In summary, functional changes in the distal colon caused by DSS treatment were more pronounced in the mucus-associated microbiota than in the microbiota present in the distal colon content.
30767541	0	37	from	Functions	81:89	arg1	Model					104:108	a Refined Model	94:108	a Refined Model of Dextran Sulfate Sodium (DSS)-Induced Colitis	94:156	Disease Development Is Accompanied by Changes in Bacterial Protein Abundance and Functions in a Refined Model of Dextran Sulfate Sodium (DSS)-Induced Colitis.
30767541	3	38	theme	chronic	518:524	arg1	colitis					538:544	chronic DSS-induced colitis	518:544	chronic DSS-induced colitis	518:544	We used a refined model of chronic DSS-induced colitis that reflects typical symptoms of the human disease without detrimental weight loss usually observed in DSS models.
30767541	1	39	theme	intestinal	258:267	arg1	inflammation					269:280	intestinal inflammation	258:280	intestinal inflammation	258:280	Using the acute dextran sulfate sodium (DSS)-induced colitis model, studies have demonstrated that intestinal inflammation is accompanied by major changes in the composition of the intestinal microbiota.
30767541	7	40	theme	sampling	1004:1011	arg1	content					1019:1025	separately sampling lumen content	993:1025	separately sampling lumen content	993:1025	Functional differences were observed between sample types demonstrating the importance of separately sampling lumen content and mucus.
30767541	9	41	theme	vitamin	1206:1212	arg1	B6					1214:1215	vitamin B6	1206:1215	vitamin B6	1206:1215	Lachnoclostridium was positively correlated to both vitamin B6 and tryptophan metabolism.
30767541	3	42	theme	typical	560:566	arg1	symptoms					568:575	typical symptoms	560:575	typical symptoms of the human disease	560:596	We used a refined model of chronic DSS-induced colitis that reflects typical symptoms of the human disease without detrimental weight loss usually observed in DSS models.
30767541	2	43	theme	functional	439:448	arg1	impact					450:455	more importantly their functional impact	416:455	more importantly their functional impact	416:455	Only little is known about the microbial changes and more importantly their functional impact in the chronic DSS colitis model.
30767541	7	44	theme	lumen	1013:1017	arg1	content					1019:1025	separately sampling lumen content	993:1025	separately sampling lumen content	993:1025	Functional differences were observed between sample types demonstrating the importance of separately sampling lumen content and mucus.
30767541	0	45	from	Abundance	67:75	arg1	Model					104:108	a Refined Model	94:108	a Refined Model of Dextran Sulfate Sodium (DSS)-Induced Colitis	94:156	Disease Development Is Accompanied by Changes in Bacterial Protein Abundance and Functions in a Refined Model of Dextran Sulfate Sodium (DSS)-Induced Colitis.
30767541	10	46	from	summary	1247:1253	arg1	pronounced					1329:1338	pronounced	1329:1338	pronounced	1329:1338	In summary, functional changes in the distal colon caused by DSS treatment were more pronounced in the mucus-associated microbiota than in the microbiota present in the distal colon content.
30767541	2	47	theme	colitis	476:482	arg1	model					484:488	the chronic DSS colitis model	460:488	the chronic DSS colitis model	460:488	Only little is known about the microbial changes and more importantly their functional impact in the chronic DSS colitis model.
30767541	0	48	theme	Bacterial	49:57	arg1	Abundance					67:75	Bacterial Protein Abundance	49:75	Bacterial Protein Abundance	49:75	Disease Development Is Accompanied by Changes in Bacterial Protein Abundance and Functions in a Refined Model of Dextran Sulfate Sodium (DSS)-Induced Colitis.
30767541	10	49	from	content	1426:1432	arg1	pronounced					1329:1338	pronounced	1329:1338	pronounced	1329:1338	In summary, functional changes in the distal colon caused by DSS treatment were more pronounced in the mucus-associated microbiota than in the microbiota present in the distal colon content.
30767541	8	50	theme	Desulfovibrio	1055:1067	arg1	abundance					1042:1050	The abundance	1038:1050	The abundance of Desulfovibrio, a sulfate-reducing bacterium,	1038:1098	The abundance of Desulfovibrio, a sulfate-reducing bacterium, was positively associated with the carbon metabolism.
30767541	0	51	theme	Protein	59:65	arg1	Abundance					67:75	Bacterial Protein Abundance	49:75	Bacterial Protein Abundance	49:75	Disease Development Is Accompanied by Changes in Bacterial Protein Abundance and Functions in a Refined Model of Dextran Sulfate Sodium (DSS)-Induced Colitis.
30767541	10	52	from	changes	1267:1273	arg1	colon					1289:1293	the distal colon	1278:1293	the distal colon caused by DSS treatment	1278:1317	In summary, functional changes in the distal colon caused by DSS treatment were more pronounced in the mucus-associated microbiota than in the microbiota present in the distal colon content.
30767541	2	53	from	impact	450:455	arg1	model					484:488	the chronic DSS colitis model	460:488	the chronic DSS colitis model	460:488	Only little is known about the microbial changes and more importantly their functional impact in the chronic DSS colitis model.
30767541	5	54	theme	Prevotella	842:851	arg1	sp					853:854	Prevotella sp	842:854	Prevotella sp	842:854	An increase of Prevotella sp.
30767541	1	55	from	changes	306:312	arg1	composition					321:331	the composition	317:331	the composition of the intestinal microbiota	317:360	Using the acute dextran sulfate sodium (DSS)-induced colitis model, studies have demonstrated that intestinal inflammation is accompanied by major changes in the composition of the intestinal microbiota.
30767541	6	56	theme	colon	865:869	arg1	content					871:877	colon content	865:877	colon content	865:877	in both colon content and mucus was observed.
30767541	2	57	from	changes	404:410	arg1	model					484:488	the chronic DSS colitis model	460:488	the chronic DSS colitis model	460:488	Only little is known about the microbial changes and more importantly their functional impact in the chronic DSS colitis model.
30767541	7	58	theme	mucus	1031:1035	arg1	importance					979:988	the importance	975:988	the importance of separately sampling lumen content and mucus	975:1035	Functional differences were observed between sample types demonstrating the importance of separately sampling lumen content and mucus.
30767541	5	59	theme	sp	853:854	arg1	increase					830:837	An increase	827:837	An increase of Prevotella sp	827:854	An increase of Prevotella sp.
30767541	0	60	theme	-Induced	141:148	arg1	Colitis					150:156	Dextran Sulfate Sodium (DSS)-Induced Colitis	113:156	Dextran Sulfate Sodium (DSS)-Induced Colitis	113:156	Disease Development Is Accompanied by Changes in Bacterial Protein Abundance and Functions in a Refined Model of Dextran Sulfate Sodium (DSS)-Induced Colitis.
30767541	3	61	theme	DSS	650:652	arg1	models					654:659	DSS models	650:659	DSS models	650:659	We used a refined model of chronic DSS-induced colitis that reflects typical symptoms of the human disease without detrimental weight loss usually observed in DSS models.
30767541	3	62	theme	human	584:588	arg1	disease					590:596	the human disease	580:596	the human disease	580:596	We used a refined model of chronic DSS-induced colitis that reflects typical symptoms of the human disease without detrimental weight loss usually observed in DSS models.
30767541	7	63	theme	sample	948:953	arg1	types					955:959	sample types	948:959	sample types demonstrating the importance of separately sampling lumen content and mucus	948:1035	Functional differences were observed between sample types demonstrating the importance of separately sampling lumen content and mucus.
30767541	3	64	theme	disease	590:596	arg1	symptoms					568:575	typical symptoms	560:575	typical symptoms of the human disease	560:596	We used a refined model of chronic DSS-induced colitis that reflects typical symptoms of the human disease without detrimental weight loss usually observed in DSS models.
30767541	9	65	theme	tryptophan	1221:1230	arg1	metabolism					1232:1241	tryptophan metabolism	1221:1241	tryptophan metabolism	1221:1241	Lachnoclostridium was positively correlated to both vitamin B6 and tryptophan metabolism.
30767541	4	66	theme	mouse	746:750	arg1	cohorts					752:758	healthy and diseased mouse cohorts	725:758	healthy and diseased mouse cohorts ( n = 12)	725:768	We sampled cecum and colon content as well as colon mucus from healthy and diseased mouse cohorts ( n = 12) and applied 16S rRNA gene sequencing and metaproteomics.
30767541	4	66	theme	mouse	746:750	arg1	n					762:762	n = 12	762:767	n = 12	762:767	We sampled cecum and colon content as well as colon mucus from healthy and diseased mouse cohorts ( n = 12) and applied 16S rRNA gene sequencing and metaproteomics.
30767541	2	67	theme	DSS	472:474	arg1	model					484:488	the chronic DSS colitis model	460:488	the chronic DSS colitis model	460:488	Only little is known about the microbial changes and more importantly their functional impact in the chronic DSS colitis model.
30767541	10	68	from	pronounced	1329:1338	arg1	microbiota					1364:1373	the mucus-associated microbiota	1343:1373	the mucus-associated microbiota	1343:1373	In summary, functional changes in the distal colon caused by DSS treatment were more pronounced in the mucus-associated microbiota than in the microbiota present in the distal colon content.
30767541	10	68	from	pronounced	1329:1338	arg1	summary					1247:1253	summary	1247:1253	summary	1247:1253	In summary, functional changes in the distal colon caused by DSS treatment were more pronounced in the mucus-associated microbiota than in the microbiota present in the distal colon content.
30767541	10	68	from	pronounced	1329:1338	arg1	content					1426:1432	the microbiota present in the distal colon content	1383:1432	the microbiota present in the distal colon content	1383:1432	In summary, functional changes in the distal colon caused by DSS treatment were more pronounced in the mucus-associated microbiota than in the microbiota present in the distal colon content.
30767541	2	69	theme	chronic	464:470	arg1	model					484:488	the chronic DSS colitis model	460:488	the chronic DSS colitis model	460:488	Only little is known about the microbial changes and more importantly their functional impact in the chronic DSS colitis model.
30767541	1	70	theme	major	300:304	arg1	changes					306:312	major changes	300:312	major changes in the composition of the intestinal microbiota	300:360	Using the acute dextran sulfate sodium (DSS)-induced colitis model, studies have demonstrated that intestinal inflammation is accompanied by major changes in the composition of the intestinal microbiota.
30767541	7	71	theme	Functional	903:912	arg1	differences					914:924	Functional differences	903:924	Functional differences	903:924	Functional differences were observed between sample types demonstrating the importance of separately sampling lumen content and mucus.
30767541	1	72	theme	-induced	203:210	arg1	model					220:224	the acute dextran sulfate sodium (DSS)-induced colitis model	165:224	the acute dextran sulfate sodium (DSS)-induced colitis model	165:224	Using the acute dextran sulfate sodium (DSS)-induced colitis model, studies have demonstrated that intestinal inflammation is accompanied by major changes in the composition of the intestinal microbiota.
29968739	5	0	theme	decomposition	1119:1131	arg1	stage					1103:1107	the initial stage	1091:1107	the initial stage of litter decomposition	1091:1131	Furthermore, the BIOENV procedure revealed that the carbon to nitrogen (C:N) ratio, carbon to phosphorus (C:P) ratio and moisture content (MC) were the most important determinants of microbial community structure in the initial stage of litter decomposition, whereas pH and the lignin concentration were the major factors influencing the microbial community structure in the later stage of litter decomposition.
29968739	3	1	theme	alpine	711:716	arg1	shrubland					718:726	alpine shrubland	711:726	alpine shrubland	711:726	The activities of ligninolytic and cellulolytic enzymes and the biomass of microbial PLFAs were higher in the initial stage of litter decomposition than in the latter stage in the three vegetation types (coniferous forest, alpine shrubland and alpine meadow).
29968739	2	2	theme	ligninolytic	352:363	arg1	activities					389:398	the ligninolytic and cellulolytic enzyme activities	348:398	the ligninolytic and cellulolytic enzyme activities of the litter horizon across an alpine treeline ecotone in the eastern Tibetan Plateau	348:485	We monitored microbial community composition using phospholipid fatty acid (PLFA) analysis and investigated the ligninolytic and cellulolytic enzyme activities of the litter horizon across an alpine treeline ecotone in the eastern Tibetan Plateau.
29968739	6	3	theme	treeline	1422:1429	arg1	ecotone					1431:1437	an alpine treeline ecotone	1412:1437	an alpine treeline ecotone in the eastern Tibetan Plateau	1412:1468	These findings indicate that litter quality drives the differentiation of microbial communities in the litter horizon across an alpine treeline ecotone in the eastern Tibetan Plateau.
29968739	0	4	theme	litter	74:79	arg1	horizon					81:87	the litter horizon	70:87	the litter horizon across an alpine treeline ecotone in the eastern Tibetan Plateau	70:152	Litter quality drives the differentiation of microbial communities in the litter horizon across an alpine treeline ecotone in the eastern Tibetan Plateau.
29968739	5	5	theme	community	1068:1076	arg1	structure					1078:1086	microbial community structure	1058:1086	microbial community structure	1058:1086	Furthermore, the BIOENV procedure revealed that the carbon to nitrogen (C:N) ratio, carbon to phosphorus (C:P) ratio and moisture content (MC) were the most important determinants of microbial community structure in the initial stage of litter decomposition, whereas pH and the lignin concentration were the major factors influencing the microbial community structure in the later stage of litter decomposition.
29968739	3	6	dep	higher	584:589	arg1	forest					703:708	coniferous forest	692:708	coniferous forest	692:708	The activities of ligninolytic and cellulolytic enzymes and the biomass of microbial PLFAs were higher in the initial stage of litter decomposition than in the latter stage in the three vegetation types (coniferous forest, alpine shrubland and alpine meadow).
29968739	3	6	dep	higher	584:589	arg1	shrubland					718:726	alpine shrubland	711:726	alpine shrubland	711:726	The activities of ligninolytic and cellulolytic enzymes and the biomass of microbial PLFAs were higher in the initial stage of litter decomposition than in the latter stage in the three vegetation types (coniferous forest, alpine shrubland and alpine meadow).
29968739	3	6	dep	higher	584:589	arg1	meadow					739:744	alpine meadow	732:744	alpine meadow	732:744	The activities of ligninolytic and cellulolytic enzymes and the biomass of microbial PLFAs were higher in the initial stage of litter decomposition than in the latter stage in the three vegetation types (coniferous forest, alpine shrubland and alpine meadow).
29968739	6	7	theme	alpine	1415:1420	arg1	ecotone					1431:1437	an alpine treeline ecotone	1412:1437	an alpine treeline ecotone in the eastern Tibetan Plateau	1412:1468	These findings indicate that litter quality drives the differentiation of microbial communities in the litter horizon across an alpine treeline ecotone in the eastern Tibetan Plateau.
29968739	5	8	theme	microbial	1213:1221	arg1	structure					1233:1241	the microbial community structure	1209:1241	the microbial community structure	1209:1241	Furthermore, the BIOENV procedure revealed that the carbon to nitrogen (C:N) ratio, carbon to phosphorus (C:P) ratio and moisture content (MC) were the most important determinants of microbial community structure in the initial stage of litter decomposition, whereas pH and the lignin concentration were the major factors influencing the microbial community structure in the later stage of litter decomposition.
29968739	6	9	theme	litter	1316:1321	arg1	quality					1323:1329	litter quality	1316:1329	litter quality	1316:1329	These findings indicate that litter quality drives the differentiation of microbial communities in the litter horizon across an alpine treeline ecotone in the eastern Tibetan Plateau.
29968739	2	10	from	ecotone	448:454	arg1	Plateau					479:485	the eastern Tibetan Plateau	459:485	the eastern Tibetan Plateau	459:485	We monitored microbial community composition using phospholipid fatty acid (PLFA) analysis and investigated the ligninolytic and cellulolytic enzyme activities of the litter horizon across an alpine treeline ecotone in the eastern Tibetan Plateau.
29968739	6	11	theme	microbial	1361:1369	arg1	communities					1371:1381	microbial communities	1361:1381	microbial communities	1361:1381	These findings indicate that litter quality drives the differentiation of microbial communities in the litter horizon across an alpine treeline ecotone in the eastern Tibetan Plateau.
29968739	3	12	theme	cellulolytic	523:534	arg1	enzymes					536:542	ligninolytic and cellulolytic enzymes	506:542	ligninolytic and cellulolytic enzymes	506:542	The activities of ligninolytic and cellulolytic enzymes and the biomass of microbial PLFAs were higher in the initial stage of litter decomposition than in the latter stage in the three vegetation types (coniferous forest, alpine shrubland and alpine meadow).
29968739	2	13	theme	phospholipid	291:302	arg1	analysis					322:329	phospholipid fatty acid (PLFA) analysis	291:329	phospholipid fatty acid (PLFA) analysis	291:329	We monitored microbial community composition using phospholipid fatty acid (PLFA) analysis and investigated the ligninolytic and cellulolytic enzyme activities of the litter horizon across an alpine treeline ecotone in the eastern Tibetan Plateau.
29968739	3	14	theme	PLFAs	573:577	arg1	activities					492:501	The activities	488:501	The activities of ligninolytic and cellulolytic enzymes	488:542	The activities of ligninolytic and cellulolytic enzymes and the biomass of microbial PLFAs were higher in the initial stage of litter decomposition than in the latter stage in the three vegetation types (coniferous forest, alpine shrubland and alpine meadow).
29968739	3	14	theme	PLFAs	573:577	arg1	biomass					552:558	the biomass	548:558	the biomass of microbial PLFAs	548:577	The activities of ligninolytic and cellulolytic enzymes and the biomass of microbial PLFAs were higher in the initial stage of litter decomposition than in the latter stage in the three vegetation types (coniferous forest, alpine shrubland and alpine meadow).
29968739	3	14	theme	PLFAs	573:577	arg1	higher					584:589	higher	584:589	higher	584:589	The activities of ligninolytic and cellulolytic enzymes and the biomass of microbial PLFAs were higher in the initial stage of litter decomposition than in the latter stage in the three vegetation types (coniferous forest, alpine shrubland and alpine meadow).
29968739	4	15	theme	Soil	748:751	arg1	structure					773:781	Soil microbial community structure	748:781	Soil microbial community structure	748:781	Soil microbial community structure varied significantly over the course of litter decomposition in the three vegetation types.
29968739	3	16	theme	ligninolytic	506:517	arg1	enzymes					536:542	ligninolytic and cellulolytic enzymes	506:542	ligninolytic and cellulolytic enzymes	506:542	The activities of ligninolytic and cellulolytic enzymes and the biomass of microbial PLFAs were higher in the initial stage of litter decomposition than in the latter stage in the three vegetation types (coniferous forest, alpine shrubland and alpine meadow).
29968739	5	17	theme	litter	1265:1270	arg1	decomposition					1272:1284	litter decomposition	1265:1284	litter decomposition	1265:1284	Furthermore, the BIOENV procedure revealed that the carbon to nitrogen (C:N) ratio, carbon to phosphorus (C:P) ratio and moisture content (MC) were the most important determinants of microbial community structure in the initial stage of litter decomposition, whereas pH and the lignin concentration were the major factors influencing the microbial community structure in the later stage of litter decomposition.
29968739	0	18	theme	treeline	106:113	arg1	ecotone					115:121	an alpine treeline ecotone	96:121	an alpine treeline ecotone in the eastern Tibetan Plateau	96:152	Litter quality drives the differentiation of microbial communities in the litter horizon across an alpine treeline ecotone in the eastern Tibetan Plateau.
29968739	6	19	from	ecotone	1431:1437	arg1	Plateau					1462:1468	the eastern Tibetan Plateau	1442:1468	the eastern Tibetan Plateau	1442:1468	These findings indicate that litter quality drives the differentiation of microbial communities in the litter horizon across an alpine treeline ecotone in the eastern Tibetan Plateau.
29968739	5	20	theme	decomposition	1272:1284	arg1	stage					1256:1260	the later stage	1246:1260	the later stage of litter decomposition	1246:1284	Furthermore, the BIOENV procedure revealed that the carbon to nitrogen (C:N) ratio, carbon to phosphorus (C:P) ratio and moisture content (MC) were the most important determinants of microbial community structure in the initial stage of litter decomposition, whereas pH and the lignin concentration were the major factors influencing the microbial community structure in the later stage of litter decomposition.
29968739	5	21	dep	nitrogen	937:944	arg1	C					947:947	C	947:947	C:N	947:949	Furthermore, the BIOENV procedure revealed that the carbon to nitrogen (C:N) ratio, carbon to phosphorus (C:P) ratio and moisture content (MC) were the most important determinants of microbial community structure in the initial stage of litter decomposition, whereas pH and the lignin concentration were the major factors influencing the microbial community structure in the later stage of litter decomposition.
29968739	5	21	dep	nitrogen	937:944	arg1	ratio					952:956	ratio	952:956	ratio	952:956	Furthermore, the BIOENV procedure revealed that the carbon to nitrogen (C:N) ratio, carbon to phosphorus (C:P) ratio and moisture content (MC) were the most important determinants of microbial community structure in the initial stage of litter decomposition, whereas pH and the lignin concentration were the major factors influencing the microbial community structure in the later stage of litter decomposition.
29968739	5	21	dep	nitrogen	937:944	arg1	C					981:981	C	981:981	C:P	981:983	Furthermore, the BIOENV procedure revealed that the carbon to nitrogen (C:N) ratio, carbon to phosphorus (C:P) ratio and moisture content (MC) were the most important determinants of microbial community structure in the initial stage of litter decomposition, whereas pH and the lignin concentration were the major factors influencing the microbial community structure in the later stage of litter decomposition.
29968739	0	22	theme	alpine	99:104	arg1	ecotone					115:121	an alpine treeline ecotone	96:121	an alpine treeline ecotone in the eastern Tibetan Plateau	96:152	Litter quality drives the differentiation of microbial communities in the litter horizon across an alpine treeline ecotone in the eastern Tibetan Plateau.
29968739	5	23	theme	carbon	959:964	arg1	phosphorus					969:978	carbon to phosphorus	959:978	carbon to phosphorus	959:978	Furthermore, the BIOENV procedure revealed that the carbon to nitrogen (C:N) ratio, carbon to phosphorus (C:P) ratio and moisture content (MC) were the most important determinants of microbial community structure in the initial stage of litter decomposition, whereas pH and the lignin concentration were the major factors influencing the microbial community structure in the later stage of litter decomposition.
29968739	5	23	theme	carbon	959:964	arg1	nitrogen					937:944	nitrogen	937:944	nitrogen	937:944	Furthermore, the BIOENV procedure revealed that the carbon to nitrogen (C:N) ratio, carbon to phosphorus (C:P) ratio and moisture content (MC) were the most important determinants of microbial community structure in the initial stage of litter decomposition, whereas pH and the lignin concentration were the major factors influencing the microbial community structure in the later stage of litter decomposition.
29968739	5	24	theme	microbial	1058:1066	arg1	structure					1078:1086	microbial community structure	1058:1086	microbial community structure	1058:1086	Furthermore, the BIOENV procedure revealed that the carbon to nitrogen (C:N) ratio, carbon to phosphorus (C:P) ratio and moisture content (MC) were the most important determinants of microbial community structure in the initial stage of litter decomposition, whereas pH and the lignin concentration were the major factors influencing the microbial community structure in the later stage of litter decomposition.
29968739	5	25	dep	C	947:947	arg1	N					949:949	N	949:949	C:N	947:949	Furthermore, the BIOENV procedure revealed that the carbon to nitrogen (C:N) ratio, carbon to phosphorus (C:P) ratio and moisture content (MC) were the most important determinants of microbial community structure in the initial stage of litter decomposition, whereas pH and the lignin concentration were the major factors influencing the microbial community structure in the later stage of litter decomposition.
29968739	2	26	theme	community	263:271	arg1	composition					273:283	microbial community composition	253:283	microbial community composition	253:283	We monitored microbial community composition using phospholipid fatty acid (PLFA) analysis and investigated the ligninolytic and cellulolytic enzyme activities of the litter horizon across an alpine treeline ecotone in the eastern Tibetan Plateau.
29968739	0	27	from	differentiation	26:40	arg1	horizon					81:87	the litter horizon	70:87	the litter horizon across an alpine treeline ecotone in the eastern Tibetan Plateau	70:152	Litter quality drives the differentiation of microbial communities in the litter horizon across an alpine treeline ecotone in the eastern Tibetan Plateau.
29968739	4	28	theme	vegetation	857:866	arg1	types					868:872	the three vegetation types	847:872	the three vegetation types	847:872	Soil microbial community structure varied significantly over the course of litter decomposition in the three vegetation types.
29968739	5	29	theme	nitrogen	937:944	arg1	ratio					986:990	nitrogen (C:N) ratio, carbon to phosphorus (C:P) ratio	937:990	nitrogen (C:N) ratio, carbon to phosphorus (C:P) ratio	937:990	Furthermore, the BIOENV procedure revealed that the carbon to nitrogen (C:N) ratio, carbon to phosphorus (C:P) ratio and moisture content (MC) were the most important determinants of microbial community structure in the initial stage of litter decomposition, whereas pH and the lignin concentration were the major factors influencing the microbial community structure in the later stage of litter decomposition.
29968739	5	30	dep	C	981:981	arg1	P					983:983	P	983:983	C:P	981:983	Furthermore, the BIOENV procedure revealed that the carbon to nitrogen (C:N) ratio, carbon to phosphorus (C:P) ratio and moisture content (MC) were the most important determinants of microbial community structure in the initial stage of litter decomposition, whereas pH and the lignin concentration were the major factors influencing the microbial community structure in the later stage of litter decomposition.
29968739	0	31	theme	Litter	0:5	arg1	quality					7:13	Litter quality	0:13	Litter quality	0:13	Litter quality drives the differentiation of microbial communities in the litter horizon across an alpine treeline ecotone in the eastern Tibetan Plateau.
29968739	6	32	theme	eastern	1446:1452	arg1	Plateau					1462:1468	the eastern Tibetan Plateau	1442:1468	the eastern Tibetan Plateau	1442:1468	These findings indicate that litter quality drives the differentiation of microbial communities in the litter horizon across an alpine treeline ecotone in the eastern Tibetan Plateau.
29968739	3	33	theme	decomposition	622:634	arg1	stage					606:610	the initial stage	594:610	the initial stage of litter decomposition	594:634	The activities of ligninolytic and cellulolytic enzymes and the biomass of microbial PLFAs were higher in the initial stage of litter decomposition than in the latter stage in the three vegetation types (coniferous forest, alpine shrubland and alpine meadow).
29968739	2	34	theme	enzyme	382:387	arg1	activities					389:398	the ligninolytic and cellulolytic enzyme activities	348:398	the ligninolytic and cellulolytic enzyme activities of the litter horizon across an alpine treeline ecotone in the eastern Tibetan Plateau	348:485	We monitored microbial community composition using phospholipid fatty acid (PLFA) analysis and investigated the ligninolytic and cellulolytic enzyme activities of the litter horizon across an alpine treeline ecotone in the eastern Tibetan Plateau.
29968739	2	35	theme	treeline	439:446	arg1	ecotone					448:454	an alpine treeline ecotone	429:454	an alpine treeline ecotone in the eastern Tibetan Plateau	429:485	We monitored microbial community composition using phospholipid fatty acid (PLFA) analysis and investigated the ligninolytic and cellulolytic enzyme activities of the litter horizon across an alpine treeline ecotone in the eastern Tibetan Plateau.
29968739	5	36	theme	litter	1112:1117	arg1	decomposition					1119:1131	litter decomposition	1112:1131	litter decomposition	1112:1131	Furthermore, the BIOENV procedure revealed that the carbon to nitrogen (C:N) ratio, carbon to phosphorus (C:P) ratio and moisture content (MC) were the most important determinants of microbial community structure in the initial stage of litter decomposition, whereas pH and the lignin concentration were the major factors influencing the microbial community structure in the later stage of litter decomposition.
29968739	6	37	from	differentiation	1342:1356	arg1	horizon					1397:1403	the litter horizon	1386:1403	the litter horizon across an alpine treeline ecotone in the eastern Tibetan Plateau	1386:1468	These findings indicate that litter quality drives the differentiation of microbial communities in the litter horizon across an alpine treeline ecotone in the eastern Tibetan Plateau.
29968739	2	38	theme	acid	310:313	arg1	analysis					322:329	phospholipid fatty acid (PLFA) analysis	291:329	phospholipid fatty acid (PLFA) analysis	291:329	We monitored microbial community composition using phospholipid fatty acid (PLFA) analysis and investigated the ligninolytic and cellulolytic enzyme activities of the litter horizon across an alpine treeline ecotone in the eastern Tibetan Plateau.
29968739	5	39	theme	later	1250:1254	arg1	stage					1256:1260	the later stage	1246:1260	the later stage of litter decomposition	1246:1284	Furthermore, the BIOENV procedure revealed that the carbon to nitrogen (C:N) ratio, carbon to phosphorus (C:P) ratio and moisture content (MC) were the most important determinants of microbial community structure in the initial stage of litter decomposition, whereas pH and the lignin concentration were the major factors influencing the microbial community structure in the later stage of litter decomposition.
29968739	1	40	theme	main	184:187	arg1	components					199:208	the main polymeric components	180:208	the main polymeric components of the forest litter horizon	180:237	Cellulose and lignin are the main polymeric components of the forest litter horizon.
29968739	1	40	theme	main	184:187	arg1	lignin					169:174	lignin	169:174	lignin	169:174	Cellulose and lignin are the main polymeric components of the forest litter horizon.
29968739	1	40	theme	main	184:187	arg1	Cellulose					155:163	Cellulose	155:163	Cellulose	155:163	Cellulose and lignin are the main polymeric components of the forest litter horizon.
29968739	2	41	theme	alpine	432:437	arg1	ecotone					448:454	an alpine treeline ecotone	429:454	an alpine treeline ecotone in the eastern Tibetan Plateau	429:485	We monitored microbial community composition using phospholipid fatty acid (PLFA) analysis and investigated the ligninolytic and cellulolytic enzyme activities of the litter horizon across an alpine treeline ecotone in the eastern Tibetan Plateau.
29968739	3	42	theme	alpine	732:737	arg1	meadow					739:744	alpine meadow	732:744	alpine meadow	732:744	The activities of ligninolytic and cellulolytic enzymes and the biomass of microbial PLFAs were higher in the initial stage of litter decomposition than in the latter stage in the three vegetation types (coniferous forest, alpine shrubland and alpine meadow).
29968739	3	43	theme	latter	648:653	arg1	stage					655:659	the latter stage	644:659	the latter stage in the three vegetation types	644:689	The activities of ligninolytic and cellulolytic enzymes and the biomass of microbial PLFAs were higher in the initial stage of litter decomposition than in the latter stage in the three vegetation types (coniferous forest, alpine shrubland and alpine meadow).
29968739	2	44	theme	fatty	304:308	arg1	acid					310:313	fatty acid	304:313	phospholipid fatty acid (PLFA) analysis	291:329	We monitored microbial community composition using phospholipid fatty acid (PLFA) analysis and investigated the ligninolytic and cellulolytic enzyme activities of the litter horizon across an alpine treeline ecotone in the eastern Tibetan Plateau.
29968739	2	44	theme	fatty	304:308	arg1	PLFA					316:319	PLFA	316:319	PLFA	316:319	We monitored microbial community composition using phospholipid fatty acid (PLFA) analysis and investigated the ligninolytic and cellulolytic enzyme activities of the litter horizon across an alpine treeline ecotone in the eastern Tibetan Plateau.
29968739	1	45	theme	polymeric	189:197	arg1	components					199:208	the main polymeric components	180:208	the main polymeric components of the forest litter horizon	180:237	Cellulose and lignin are the main polymeric components of the forest litter horizon.
29968739	1	45	theme	polymeric	189:197	arg1	lignin					169:174	lignin	169:174	lignin	169:174	Cellulose and lignin are the main polymeric components of the forest litter horizon.
29968739	1	45	theme	polymeric	189:197	arg1	Cellulose					155:163	Cellulose	155:163	Cellulose	155:163	Cellulose and lignin are the main polymeric components of the forest litter horizon.
29968739	0	46	theme	eastern	130:136	arg1	Plateau					146:152	the eastern Tibetan Plateau	126:152	the eastern Tibetan Plateau	126:152	Litter quality drives the differentiation of microbial communities in the litter horizon across an alpine treeline ecotone in the eastern Tibetan Plateau.
29968739	3	47	theme	initial	598:604	arg1	stage					606:610	the initial stage	594:610	the initial stage of litter decomposition	594:634	The activities of ligninolytic and cellulolytic enzymes and the biomass of microbial PLFAs were higher in the initial stage of litter decomposition than in the latter stage in the three vegetation types (coniferous forest, alpine shrubland and alpine meadow).
29968739	2	48	theme	horizon	414:420	arg1	activities					389:398	the ligninolytic and cellulolytic enzyme activities	348:398	the ligninolytic and cellulolytic enzyme activities of the litter horizon across an alpine treeline ecotone in the eastern Tibetan Plateau	348:485	We monitored microbial community composition using phospholipid fatty acid (PLFA) analysis and investigated the ligninolytic and cellulolytic enzyme activities of the litter horizon across an alpine treeline ecotone in the eastern Tibetan Plateau.
29968739	2	49	theme	Tibetan	471:477	arg1	Plateau					479:485	the eastern Tibetan Plateau	459:485	the eastern Tibetan Plateau	459:485	We monitored microbial community composition using phospholipid fatty acid (PLFA) analysis and investigated the ligninolytic and cellulolytic enzyme activities of the litter horizon across an alpine treeline ecotone in the eastern Tibetan Plateau.
29968739	6	50	theme	communities	1371:1381	arg1	differentiation					1342:1356	the differentiation	1338:1356	the differentiation of microbial communities in the litter horizon across an alpine treeline ecotone in the eastern Tibetan Plateau	1338:1468	These findings indicate that litter quality drives the differentiation of microbial communities in the litter horizon across an alpine treeline ecotone in the eastern Tibetan Plateau.
29968739	6	51	theme	litter	1390:1395	arg1	horizon					1397:1403	the litter horizon	1386:1403	the litter horizon across an alpine treeline ecotone in the eastern Tibetan Plateau	1386:1468	These findings indicate that litter quality drives the differentiation of microbial communities in the litter horizon across an alpine treeline ecotone in the eastern Tibetan Plateau.
29968739	2	52	theme	litter	407:412	arg1	horizon					414:420	the litter horizon	403:420	the litter horizon across an alpine treeline ecotone in the eastern Tibetan Plateau	403:485	We monitored microbial community composition using phospholipid fatty acid (PLFA) analysis and investigated the ligninolytic and cellulolytic enzyme activities of the litter horizon across an alpine treeline ecotone in the eastern Tibetan Plateau.
29968739	0	53	theme	communities	55:65	arg1	differentiation					26:40	the differentiation	22:40	the differentiation of microbial communities in the litter horizon across an alpine treeline ecotone in the eastern Tibetan Plateau	22:152	Litter quality drives the differentiation of microbial communities in the litter horizon across an alpine treeline ecotone in the eastern Tibetan Plateau.
29968739	6	54	theme	Tibetan	1454:1460	arg1	Plateau					1462:1468	the eastern Tibetan Plateau	1442:1468	the eastern Tibetan Plateau	1442:1468	These findings indicate that litter quality drives the differentiation of microbial communities in the litter horizon across an alpine treeline ecotone in the eastern Tibetan Plateau.
29968739	4	55	theme	decomposition	830:842	arg1	course					813:818	the course	809:818	the course of litter decomposition in the three vegetation types	809:872	Soil microbial community structure varied significantly over the course of litter decomposition in the three vegetation types.
29968739	5	56	theme	important	1032:1040	arg1	determinants					1042:1053	the most important determinants	1023:1053	the most important determinants of microbial community structure	1023:1086	Furthermore, the BIOENV procedure revealed that the carbon to nitrogen (C:N) ratio, carbon to phosphorus (C:P) ratio and moisture content (MC) were the most important determinants of microbial community structure in the initial stage of litter decomposition, whereas pH and the lignin concentration were the major factors influencing the microbial community structure in the later stage of litter decomposition.
29968739	5	56	theme	important	1032:1040	arg1	carbon					927:932	the carbon	923:932	the carbon to nitrogen (C:N) ratio, carbon to phosphorus (C:P) ratio and moisture content (MC)	923:1016	Furthermore, the BIOENV procedure revealed that the carbon to nitrogen (C:N) ratio, carbon to phosphorus (C:P) ratio and moisture content (MC) were the most important determinants of microbial community structure in the initial stage of litter decomposition, whereas pH and the lignin concentration were the major factors influencing the microbial community structure in the later stage of litter decomposition.
29968739	0	57	theme	microbial	45:53	arg1	communities					55:65	microbial communities	45:65	microbial communities	45:65	Litter quality drives the differentiation of microbial communities in the litter horizon across an alpine treeline ecotone in the eastern Tibetan Plateau.
29968739	5	58	theme	initial	1095:1101	arg1	stage					1103:1107	the initial stage	1091:1107	the initial stage of litter decomposition	1091:1131	Furthermore, the BIOENV procedure revealed that the carbon to nitrogen (C:N) ratio, carbon to phosphorus (C:P) ratio and moisture content (MC) were the most important determinants of microbial community structure in the initial stage of litter decomposition, whereas pH and the lignin concentration were the major factors influencing the microbial community structure in the later stage of litter decomposition.
29968739	0	59	theme	Tibetan	138:144	arg1	Plateau					146:152	the eastern Tibetan Plateau	126:152	the eastern Tibetan Plateau	126:152	Litter quality drives the differentiation of microbial communities in the litter horizon across an alpine treeline ecotone in the eastern Tibetan Plateau.
29968739	4	60	theme	litter	823:828	arg1	decomposition					830:842	litter decomposition	823:842	litter decomposition	823:842	Soil microbial community structure varied significantly over the course of litter decomposition in the three vegetation types.
29968739	3	61	from	stage	655:659	arg1	types					685:689	the three vegetation types	664:689	the three vegetation types	664:689	The activities of ligninolytic and cellulolytic enzymes and the biomass of microbial PLFAs were higher in the initial stage of litter decomposition than in the latter stage in the three vegetation types (coniferous forest, alpine shrubland and alpine meadow).
29968739	3	62	theme	litter	615:620	arg1	decomposition					622:634	litter decomposition	615:634	litter decomposition	615:634	The activities of ligninolytic and cellulolytic enzymes and the biomass of microbial PLFAs were higher in the initial stage of litter decomposition than in the latter stage in the three vegetation types (coniferous forest, alpine shrubland and alpine meadow).
29968739	3	63	theme	vegetation	674:683	arg1	types					685:689	the three vegetation types	664:689	the three vegetation types	664:689	The activities of ligninolytic and cellulolytic enzymes and the biomass of microbial PLFAs were higher in the initial stage of litter decomposition than in the latter stage in the three vegetation types (coniferous forest, alpine shrubland and alpine meadow).
29968739	2	64	theme	microbial	253:261	arg1	composition					273:283	microbial community composition	253:283	microbial community composition	253:283	We monitored microbial community composition using phospholipid fatty acid (PLFA) analysis and investigated the ligninolytic and cellulolytic enzyme activities of the litter horizon across an alpine treeline ecotone in the eastern Tibetan Plateau.
29968739	4	65	from	course	813:818	arg1	types					868:872	the three vegetation types	847:872	the three vegetation types	847:872	Soil microbial community structure varied significantly over the course of litter decomposition in the three vegetation types.
29968739	3	66	theme	microbial	563:571	arg1	PLFAs					573:577	microbial PLFAs	563:577	microbial PLFAs	563:577	The activities of ligninolytic and cellulolytic enzymes and the biomass of microbial PLFAs were higher in the initial stage of litter decomposition than in the latter stage in the three vegetation types (coniferous forest, alpine shrubland and alpine meadow).
29968739	5	67	theme	lignin	1153:1158	arg1	concentration					1160:1172	the lignin concentration	1149:1172	the lignin concentration	1149:1172	Furthermore, the BIOENV procedure revealed that the carbon to nitrogen (C:N) ratio, carbon to phosphorus (C:P) ratio and moisture content (MC) were the most important determinants of microbial community structure in the initial stage of litter decomposition, whereas pH and the lignin concentration were the major factors influencing the microbial community structure in the later stage of litter decomposition.
29968739	5	67	theme	lignin	1153:1158	arg1	factors					1189:1195	the major factors	1179:1195	the major factors influencing the microbial community structure in the later stage of litter decomposition	1179:1284	Furthermore, the BIOENV procedure revealed that the carbon to nitrogen (C:N) ratio, carbon to phosphorus (C:P) ratio and moisture content (MC) were the most important determinants of microbial community structure in the initial stage of litter decomposition, whereas pH and the lignin concentration were the major factors influencing the microbial community structure in the later stage of litter decomposition.
29968739	5	67	theme	lignin	1153:1158	arg1	pH					1142:1143	pH	1142:1143	pH	1142:1143	Furthermore, the BIOENV procedure revealed that the carbon to nitrogen (C:N) ratio, carbon to phosphorus (C:P) ratio and moisture content (MC) were the most important determinants of microbial community structure in the initial stage of litter decomposition, whereas pH and the lignin concentration were the major factors influencing the microbial community structure in the later stage of litter decomposition.
29968739	4	68	theme	community	763:771	arg1	structure					773:781	Soil microbial community structure	748:781	Soil microbial community structure	748:781	Soil microbial community structure varied significantly over the course of litter decomposition in the three vegetation types.
29968739	5	69	theme	moisture	996:1003	arg1	MC					1014:1015	MC	1014:1015	MC	1014:1015	Furthermore, the BIOENV procedure revealed that the carbon to nitrogen (C:N) ratio, carbon to phosphorus (C:P) ratio and moisture content (MC) were the most important determinants of microbial community structure in the initial stage of litter decomposition, whereas pH and the lignin concentration were the major factors influencing the microbial community structure in the later stage of litter decomposition.
29968739	5	69	theme	moisture	996:1003	arg1	content					1005:1011	moisture content	996:1011	moisture content (MC)	996:1016	Furthermore, the BIOENV procedure revealed that the carbon to nitrogen (C:N) ratio, carbon to phosphorus (C:P) ratio and moisture content (MC) were the most important determinants of microbial community structure in the initial stage of litter decomposition, whereas pH and the lignin concentration were the major factors influencing the microbial community structure in the later stage of litter decomposition.
29968739	4	70	theme	microbial	753:761	arg1	structure					773:781	Soil microbial community structure	748:781	Soil microbial community structure	748:781	Soil microbial community structure varied significantly over the course of litter decomposition in the three vegetation types.
29968739	5	71	theme	BIOENV	892:897	arg1	procedure					899:907	the BIOENV procedure	888:907	the BIOENV procedure	888:907	Furthermore, the BIOENV procedure revealed that the carbon to nitrogen (C:N) ratio, carbon to phosphorus (C:P) ratio and moisture content (MC) were the most important determinants of microbial community structure in the initial stage of litter decomposition, whereas pH and the lignin concentration were the major factors influencing the microbial community structure in the later stage of litter decomposition.
29968739	1	72	theme	forest	217:222	arg1	horizon					231:237	the forest litter horizon	213:237	the forest litter horizon	213:237	Cellulose and lignin are the main polymeric components of the forest litter horizon.
29968739	5	73	from	determinants	1042:1053	arg1	stage					1103:1107	the initial stage	1091:1107	the initial stage of litter decomposition	1091:1131	Furthermore, the BIOENV procedure revealed that the carbon to nitrogen (C:N) ratio, carbon to phosphorus (C:P) ratio and moisture content (MC) were the most important determinants of microbial community structure in the initial stage of litter decomposition, whereas pH and the lignin concentration were the major factors influencing the microbial community structure in the later stage of litter decomposition.
29968739	5	74	theme	structure	1078:1086	arg1	determinants					1042:1053	the most important determinants	1023:1053	the most important determinants of microbial community structure	1023:1086	Furthermore, the BIOENV procedure revealed that the carbon to nitrogen (C:N) ratio, carbon to phosphorus (C:P) ratio and moisture content (MC) were the most important determinants of microbial community structure in the initial stage of litter decomposition, whereas pH and the lignin concentration were the major factors influencing the microbial community structure in the later stage of litter decomposition.
29968739	5	74	theme	structure	1078:1086	arg1	carbon					927:932	the carbon	923:932	the carbon to nitrogen (C:N) ratio, carbon to phosphorus (C:P) ratio and moisture content (MC)	923:1016	Furthermore, the BIOENV procedure revealed that the carbon to nitrogen (C:N) ratio, carbon to phosphorus (C:P) ratio and moisture content (MC) were the most important determinants of microbial community structure in the initial stage of litter decomposition, whereas pH and the lignin concentration were the major factors influencing the microbial community structure in the later stage of litter decomposition.
29968739	5	75	dep	phosphorus	969:978	arg1	to					966:967	to	966:967	to	966:967	Furthermore, the BIOENV procedure revealed that the carbon to nitrogen (C:N) ratio, carbon to phosphorus (C:P) ratio and moisture content (MC) were the most important determinants of microbial community structure in the initial stage of litter decomposition, whereas pH and the lignin concentration were the major factors influencing the microbial community structure in the later stage of litter decomposition.
29968739	2	76	theme	cellulolytic	369:380	arg1	activities					389:398	the ligninolytic and cellulolytic enzyme activities	348:398	the ligninolytic and cellulolytic enzyme activities of the litter horizon across an alpine treeline ecotone in the eastern Tibetan Plateau	348:485	We monitored microbial community composition using phospholipid fatty acid (PLFA) analysis and investigated the ligninolytic and cellulolytic enzyme activities of the litter horizon across an alpine treeline ecotone in the eastern Tibetan Plateau.
29968739	5	77	theme	community	1223:1231	arg1	structure					1233:1241	the microbial community structure	1209:1241	the microbial community structure	1209:1241	Furthermore, the BIOENV procedure revealed that the carbon to nitrogen (C:N) ratio, carbon to phosphorus (C:P) ratio and moisture content (MC) were the most important determinants of microbial community structure in the initial stage of litter decomposition, whereas pH and the lignin concentration were the major factors influencing the microbial community structure in the later stage of litter decomposition.
29968739	1	78	theme	litter	224:229	arg1	horizon					231:237	the forest litter horizon	213:237	the forest litter horizon	213:237	Cellulose and lignin are the main polymeric components of the forest litter horizon.
29968739	2	79	theme	eastern	463:469	arg1	Plateau					479:485	the eastern Tibetan Plateau	459:485	the eastern Tibetan Plateau	459:485	We monitored microbial community composition using phospholipid fatty acid (PLFA) analysis and investigated the ligninolytic and cellulolytic enzyme activities of the litter horizon across an alpine treeline ecotone in the eastern Tibetan Plateau.
29968739	3	80	theme	coniferous	692:701	arg1	forest					703:708	coniferous forest	692:708	coniferous forest	692:708	The activities of ligninolytic and cellulolytic enzymes and the biomass of microbial PLFAs were higher in the initial stage of litter decomposition than in the latter stage in the three vegetation types (coniferous forest, alpine shrubland and alpine meadow).
29968739	0	81	from	ecotone	115:121	arg1	Plateau					146:152	the eastern Tibetan Plateau	126:152	the eastern Tibetan Plateau	126:152	Litter quality drives the differentiation of microbial communities in the litter horizon across an alpine treeline ecotone in the eastern Tibetan Plateau.
29968739	3	82	theme	enzymes	536:542	arg1	activities					492:501	The activities	488:501	The activities of ligninolytic and cellulolytic enzymes	488:542	The activities of ligninolytic and cellulolytic enzymes and the biomass of microbial PLFAs were higher in the initial stage of litter decomposition than in the latter stage in the three vegetation types (coniferous forest, alpine shrubland and alpine meadow).
29968739	3	82	theme	enzymes	536:542	arg1	biomass					552:558	the biomass	548:558	the biomass of microbial PLFAs	548:577	The activities of ligninolytic and cellulolytic enzymes and the biomass of microbial PLFAs were higher in the initial stage of litter decomposition than in the latter stage in the three vegetation types (coniferous forest, alpine shrubland and alpine meadow).
29968739	3	82	theme	enzymes	536:542	arg1	higher					584:589	higher	584:589	higher	584:589	The activities of ligninolytic and cellulolytic enzymes and the biomass of microbial PLFAs were higher in the initial stage of litter decomposition than in the latter stage in the three vegetation types (coniferous forest, alpine shrubland and alpine meadow).
29968739	5	83	theme	major	1183:1187	arg1	concentration					1160:1172	the lignin concentration	1149:1172	the lignin concentration	1149:1172	Furthermore, the BIOENV procedure revealed that the carbon to nitrogen (C:N) ratio, carbon to phosphorus (C:P) ratio and moisture content (MC) were the most important determinants of microbial community structure in the initial stage of litter decomposition, whereas pH and the lignin concentration were the major factors influencing the microbial community structure in the later stage of litter decomposition.
29968739	5	83	theme	major	1183:1187	arg1	factors					1189:1195	the major factors	1179:1195	the major factors influencing the microbial community structure in the later stage of litter decomposition	1179:1284	Furthermore, the BIOENV procedure revealed that the carbon to nitrogen (C:N) ratio, carbon to phosphorus (C:P) ratio and moisture content (MC) were the most important determinants of microbial community structure in the initial stage of litter decomposition, whereas pH and the lignin concentration were the major factors influencing the microbial community structure in the later stage of litter decomposition.
29968739	5	83	theme	major	1183:1187	arg1	pH					1142:1143	pH	1142:1143	pH	1142:1143	Furthermore, the BIOENV procedure revealed that the carbon to nitrogen (C:N) ratio, carbon to phosphorus (C:P) ratio and moisture content (MC) were the most important determinants of microbial community structure in the initial stage of litter decomposition, whereas pH and the lignin concentration were the major factors influencing the microbial community structure in the later stage of litter decomposition.
29968739	1	84	theme	horizon	231:237	arg1	components					199:208	the main polymeric components	180:208	the main polymeric components of the forest litter horizon	180:237	Cellulose and lignin are the main polymeric components of the forest litter horizon.
29968739	1	84	theme	horizon	231:237	arg1	lignin					169:174	lignin	169:174	lignin	169:174	Cellulose and lignin are the main polymeric components of the forest litter horizon.
29968739	1	84	theme	horizon	231:237	arg1	Cellulose					155:163	Cellulose	155:163	Cellulose	155:163	Cellulose and lignin are the main polymeric components of the forest litter horizon.
30810637	4	0	theme	LTA	842:844	arg1	µg/mL					811:815	1 µg/mL	809:815	1 µg/mL of Enterococcus faecalis LTA	809:844	Further, cells were primed with 1 µg/mL of Enterococcus faecalis LTA for 7 days prior to the viability test with 1,000 µg/mL of each substance.
30810637	8	1	from	days	1246:1249	arg1	APC					1231:1233	APC	1231:1233	APC at 5 and 7 days	1231:1249	CMC was found toxic for APC at 5 and 7 days, whereas mCMC did not affect the cell viability.
30810637	2	2	theme	hydroxide	557:565	arg1	paste					507:511	a paste	505:511	a paste of Ciprofloxacin, Metronidazole and Calcium hydroxide (CMC - 1:1:2) and modified CMC (mCMC - 2:2:1)	505:611	MATERIAL AND METHODS Human APC were tested for in vitro cytotoxicity of modified Triple Antibiotic Paste (mTAP - Ciprofloxacin, Metronidazole and Cefaclor at 1:1:1) and of a paste of Ciprofloxacin, Metronidazole and Calcium hydroxide (CMC - 1:1:2) and modified CMC (mCMC - 2:2:1) by using MTT assay.
30810637	13	3	theme	topic	1834:1838	arg1	antimicrobials					1840:1853	topic antimicrobials	1834:1853	topic antimicrobials	1834:1853	CONCLUSION mTAP showed higher cytotoxicity than CMC and mCMC and the effect of topic antimicrobials might differ when tested against apical papilla cells under physiological or activated conditions.
30810637	1	4	theme	composition	235:245	arg1	effects					171:177	the cytotoxic effects	157:177	the cytotoxic effects of modified triple antibiotic paste and an experimental composition using calcium hydroxide on lipoteichoic acid (LTA)-primed apical papilla cells (APC)	157:330	OBJECTIVE The aim of this study was to investigate the cytotoxic effects of modified triple antibiotic paste and an experimental composition using calcium hydroxide on lipoteichoic acid (LTA)-primed apical papilla cells (APC).
30810637	2	5	dep	Ciprofloxacin	516:528	arg1	CMC					568:570	CMC	568:570	CMC	568:570	MATERIAL AND METHODS Human APC were tested for in vitro cytotoxicity of modified Triple Antibiotic Paste (mTAP - Ciprofloxacin, Metronidazole and Cefaclor at 1:1:1) and of a paste of Ciprofloxacin, Metronidazole and Calcium hydroxide (CMC - 1:1:2) and modified CMC (mCMC - 2:2:1) by using MTT assay.
30810637	2	5	dep	Ciprofloxacin	516:528	arg1	1:1:2					574:578	1:1:2	574:578	1:1:2	574:578	MATERIAL AND METHODS Human APC were tested for in vitro cytotoxicity of modified Triple Antibiotic Paste (mTAP - Ciprofloxacin, Metronidazole and Cefaclor at 1:1:1) and of a paste of Ciprofloxacin, Metronidazole and Calcium hydroxide (CMC - 1:1:2) and modified CMC (mCMC - 2:2:1) by using MTT assay.
30810637	7	6	theme	experimental	1185:1196	arg1	periods					1198:1204	all experimental periods	1181:1204	all experimental periods	1181:1204	RESULTS In the first assay, the higher cytotoxic rates were reached by mTAP for all experimental periods.
30810637	2	7	theme	Calcium	549:555	arg1	hydroxide					557:565	Calcium hydroxide	549:565	Calcium hydroxide	549:565	MATERIAL AND METHODS Human APC were tested for in vitro cytotoxicity of modified Triple Antibiotic Paste (mTAP - Ciprofloxacin, Metronidazole and Cefaclor at 1:1:1) and of a paste of Ciprofloxacin, Metronidazole and Calcium hydroxide (CMC - 1:1:2) and modified CMC (mCMC - 2:2:1) by using MTT assay.
30810637	2	8	theme	CMC	594:596	arg1	paste					507:511	a paste	505:511	a paste of Ciprofloxacin, Metronidazole and Calcium hydroxide (CMC - 1:1:2) and modified CMC (mCMC - 2:2:1)	505:611	MATERIAL AND METHODS Human APC were tested for in vitro cytotoxicity of modified Triple Antibiotic Paste (mTAP - Ciprofloxacin, Metronidazole and Cefaclor at 1:1:1) and of a paste of Ciprofloxacin, Metronidazole and Calcium hydroxide (CMC - 1:1:2) and modified CMC (mCMC - 2:2:1) by using MTT assay.
30810637	10	9	theme	LTA-untreated	1498:1510	arg1	ones					1512:1515	LTA-untreated ones	1498:1515	LTA-untreated ones	1498:1515	In the second assay, when considering the condition with medium only, LTA-primed cells significantly proliferated in comparison to LTA-untreated ones.
30810637	2	10	dep	CMC	594:596	arg1	mCMC					599:602	mCMC	599:602	mCMC	599:602	MATERIAL AND METHODS Human APC were tested for in vitro cytotoxicity of modified Triple Antibiotic Paste (mTAP - Ciprofloxacin, Metronidazole and Cefaclor at 1:1:1) and of a paste of Ciprofloxacin, Metronidazole and Calcium hydroxide (CMC - 1:1:2) and modified CMC (mCMC - 2:2:1) by using MTT assay.
30810637	2	10	dep	CMC	594:596	arg1	2:2:1					606:610	2:2:1	606:610	2:2:1	606:610	MATERIAL AND METHODS Human APC were tested for in vitro cytotoxicity of modified Triple Antibiotic Paste (mTAP - Ciprofloxacin, Metronidazole and Cefaclor at 1:1:1) and of a paste of Ciprofloxacin, Metronidazole and Calcium hydroxide (CMC - 1:1:2) and modified CMC (mCMC - 2:2:1) by using MTT assay.
30810637	2	11	dep	Paste	432:436	arg1	Ciprofloxacin					446:458	Ciprofloxacin	446:458	Ciprofloxacin	446:458	MATERIAL AND METHODS Human APC were tested for in vitro cytotoxicity of modified Triple Antibiotic Paste (mTAP - Ciprofloxacin, Metronidazole and Cefaclor at 1:1:1) and of a paste of Ciprofloxacin, Metronidazole and Calcium hydroxide (CMC - 1:1:2) and modified CMC (mCMC - 2:2:1) by using MTT assay.
30810637	2	11	dep	Paste	432:436	arg1	mTAP					439:442	mTAP	439:442	mTAP	439:442	MATERIAL AND METHODS Human APC were tested for in vitro cytotoxicity of modified Triple Antibiotic Paste (mTAP - Ciprofloxacin, Metronidazole and Cefaclor at 1:1:1) and of a paste of Ciprofloxacin, Metronidazole and Calcium hydroxide (CMC - 1:1:2) and modified CMC (mCMC - 2:2:1) by using MTT assay.
30810637	0	12	from	Cytotoxicity	0:11	arg1	cells					55:59	apical papilla cells	40:59	apical papilla cells	40:59	Cytotoxicity of intracanal dressings on apical papilla cells differ upon activation with E. faecalis LTA.
30810637	3	13	from	µg/mL	684:688	arg1	DMEM					670:673	DMEM	670:673	DMEM at 1,000 µg/mL and ¼ serially diluted before being kept in contact with cells for 1, 3, 5 and 7 days	670:774	The substances were reconstituted in DMEM at 1,000 µg/mL and ¼ serially diluted before being kept in contact with cells for 1, 3, 5 and 7 days.
30810637	0	14	with	activation	73:82	arg1	LTA					101:103	E. faecalis LTA	89:103	E. faecalis LTA	89:103	Cytotoxicity of intracanal dressings on apical papilla cells differ upon activation with E. faecalis LTA.
30810637	7	15	theme	higher	1133:1138	arg1	rates					1150:1154	the higher cytotoxic rates	1129:1154	the higher cytotoxic rates	1129:1154	RESULTS In the first assay, the higher cytotoxic rates were reached by mTAP for all experimental periods.
30810637	1	16	theme	calcium	253:259	arg1	hydroxide					261:269	calcium hydroxide	253:269	calcium hydroxide	253:269	OBJECTIVE The aim of this study was to investigate the cytotoxic effects of modified triple antibiotic paste and an experimental composition using calcium hydroxide on lipoteichoic acid (LTA)-primed apical papilla cells (APC).
30810637	0	17	theme	E.	89:90	arg1	LTA					101:103	E. faecalis LTA	89:103	E. faecalis LTA	89:103	Cytotoxicity of intracanal dressings on apical papilla cells differ upon activation with E. faecalis LTA.
30810637	2	18	theme	Metronidazole	531:543	arg1	paste					507:511	a paste	505:511	a paste of Ciprofloxacin, Metronidazole and Calcium hydroxide (CMC - 1:1:2) and modified CMC (mCMC - 2:2:1)	505:611	MATERIAL AND METHODS Human APC were tested for in vitro cytotoxicity of modified Triple Antibiotic Paste (mTAP - Ciprofloxacin, Metronidazole and Cefaclor at 1:1:1) and of a paste of Ciprofloxacin, Metronidazole and Calcium hydroxide (CMC - 1:1:2) and modified CMC (mCMC - 2:2:1) by using MTT assay.
30810637	2	19	theme	in	380:381	arg1	cytotoxicity					389:400	in vitro cytotoxicity	380:400	in vitro cytotoxicity of modified Triple Antibiotic Paste (mTAP - Ciprofloxacin, Metronidazole and Cefaclor at 1:1:1) and of a paste of Ciprofloxacin, Metronidazole and Calcium hydroxide (CMC - 1:1:2) and modified CMC (mCMC - 2:2:1)	380:611	MATERIAL AND METHODS Human APC were tested for in vitro cytotoxicity of modified Triple Antibiotic Paste (mTAP - Ciprofloxacin, Metronidazole and Cefaclor at 1:1:1) and of a paste of Ciprofloxacin, Metronidazole and Calcium hydroxide (CMC - 1:1:2) and modified CMC (mCMC - 2:2:1) by using MTT assay.
30810637	1	20	dep	OBJECTIVE	106:114	arg1	was					138:140	was	138:140	was to investigate the cytotoxic effects of modified triple antibiotic paste and an experimental composition using calcium hydroxide on lipoteichoic acid (LTA)-primed apical papilla cells (APC)	138:330	OBJECTIVE The aim of this study was to investigate the cytotoxic effects of modified triple antibiotic paste and an experimental composition using calcium hydroxide on lipoteichoic acid (LTA)-primed apical papilla cells (APC).
30810637	2	21	theme	Human	354:358	arg1	APC					360:362	Human APC	354:362	Human APC	354:362	MATERIAL AND METHODS Human APC were tested for in vitro cytotoxicity of modified Triple Antibiotic Paste (mTAP - Ciprofloxacin, Metronidazole and Cefaclor at 1:1:1) and of a paste of Ciprofloxacin, Metronidazole and Calcium hydroxide (CMC - 1:1:2) and modified CMC (mCMC - 2:2:1) by using MTT assay.
30810637	11	22	theme	LTA-primed	1669:1678	arg1	APC					1680:1682	LTA-primed APC	1669:1682	LTA-primed APC	1669:1682	At this context, mTAP and CMC showed similar cytotoxicity than the observed for LTA-untreated cells, while mCMC was shown cytotoxic at 7 days only for LTA-primed APC.
30810637	2	23	theme	Antibiotic	421:430	arg1	Paste					432:436	modified Triple Antibiotic Paste	405:436	modified Triple Antibiotic Paste (mTAP - Ciprofloxacin, Metronidazole and Cefaclor at 1:1:1)	405:496	MATERIAL AND METHODS Human APC were tested for in vitro cytotoxicity of modified Triple Antibiotic Paste (mTAP - Ciprofloxacin, Metronidazole and Cefaclor at 1:1:1) and of a paste of Ciprofloxacin, Metronidazole and Calcium hydroxide (CMC - 1:1:2) and modified CMC (mCMC - 2:2:1) by using MTT assay.
30810637	5	24	theme	one-way	962:968	arg1	analysis					970:977	one-way analysis	962:977	one-way analysis of variance (ANOVA)	962:997	Statistical analysis was performed using one-way analysis of variance (ANOVA) and two-way ANOVA respectively followed by Tukey's post-test.
30810637	13	25	theme	papilla	1895:1901	arg1	cells					1903:1907	apical papilla cells	1888:1907	apical papilla cells	1888:1907	CONCLUSION mTAP showed higher cytotoxicity than CMC and mCMC and the effect of topic antimicrobials might differ when tested against apical papilla cells under physiological or activated conditions.
30810637	0	26	dep	E.	89:90	arg1	faecalis					92:99	faecalis	92:99	faecalis	92:99	Cytotoxicity of intracanal dressings on apical papilla cells differ upon activation with E. faecalis LTA.
30810637	2	27	theme	Triple	414:419	arg1	Paste					432:436	modified Triple Antibiotic Paste	405:436	modified Triple Antibiotic Paste (mTAP - Ciprofloxacin, Metronidazole and Cefaclor at 1:1:1)	405:496	MATERIAL AND METHODS Human APC were tested for in vitro cytotoxicity of modified Triple Antibiotic Paste (mTAP - Ciprofloxacin, Metronidazole and Cefaclor at 1:1:1) and of a paste of Ciprofloxacin, Metronidazole and Calcium hydroxide (CMC - 1:1:2) and modified CMC (mCMC - 2:2:1) by using MTT assay.
30810637	4	28	theme	faecalis	833:840	arg1	LTA					842:844	Enterococcus faecalis LTA	820:844	Enterococcus faecalis LTA	820:844	Further, cells were primed with 1 µg/mL of Enterococcus faecalis LTA for 7 days prior to the viability test with 1,000 µg/mL of each substance.
30810637	10	29	theme	second	1374:1379	arg1	assay					1381:1385	the second assay	1370:1385	the second assay	1370:1385	In the second assay, when considering the condition with medium only, LTA-primed cells significantly proliferated in comparison to LTA-untreated ones.
30810637	1	30	theme	cytotoxic	161:169	arg1	effects					171:177	the cytotoxic effects	157:177	the cytotoxic effects of modified triple antibiotic paste and an experimental composition using calcium hydroxide on lipoteichoic acid (LTA)-primed apical papilla cells (APC)	157:330	OBJECTIVE The aim of this study was to investigate the cytotoxic effects of modified triple antibiotic paste and an experimental composition using calcium hydroxide on lipoteichoic acid (LTA)-primed apical papilla cells (APC).
30810637	2	31	theme	modified	405:412	arg1	Paste					432:436	modified Triple Antibiotic Paste	405:436	modified Triple Antibiotic Paste (mTAP - Ciprofloxacin, Metronidazole and Cefaclor at 1:1:1)	405:496	MATERIAL AND METHODS Human APC were tested for in vitro cytotoxicity of modified Triple Antibiotic Paste (mTAP - Ciprofloxacin, Metronidazole and Cefaclor at 1:1:1) and of a paste of Ciprofloxacin, Metronidazole and Calcium hydroxide (CMC - 1:1:2) and modified CMC (mCMC - 2:2:1) by using MTT assay.
30810637	5	32	theme	two-way	1003:1009	arg1	ANOVA					1011:1015	two-way ANOVA	1003:1015	two-way ANOVA respectively followed by Tukey's post-test	1003:1058	Statistical analysis was performed using one-way analysis of variance (ANOVA) and two-way ANOVA respectively followed by Tukey's post-test.
30810637	10	33	with	condition	1409:1417	arg1	medium					1424:1429	medium	1424:1429	medium	1424:1429	In the second assay, when considering the condition with medium only, LTA-primed cells significantly proliferated in comparison to LTA-untreated ones.
30810637	9	34	theme	cellular	1343:1350	arg1	proliferation					1352:1364	some cellular proliferation	1338:1364	some cellular proliferation	1338:1364	Only CMC and mCMC were able to induce some cellular proliferation.
30810637	0	35	theme	dressings	27:35	arg1	Cytotoxicity					0:11	Cytotoxicity	0:11	Cytotoxicity of intracanal dressings on apical papilla cells	0:59	Cytotoxicity of intracanal dressings on apical papilla cells differ upon activation with E. faecalis LTA.
30810637	1	36	theme	lipoteichoic	274:285	arg1	acid					287:290	lipoteichoic acid	274:290	lipoteichoic acid (LTA)-primed apical papilla cells (APC)	274:330	OBJECTIVE The aim of this study was to investigate the cytotoxic effects of modified triple antibiotic paste and an experimental composition using calcium hydroxide on lipoteichoic acid (LTA)-primed apical papilla cells (APC).
30810637	1	36	theme	lipoteichoic	274:285	arg1	LTA					293:295	LTA	293:295	LTA	293:295	OBJECTIVE The aim of this study was to investigate the cytotoxic effects of modified triple antibiotic paste and an experimental composition using calcium hydroxide on lipoteichoic acid (LTA)-primed apical papilla cells (APC).
30810637	13	37	theme	activated	1932:1940	arg1	conditions					1942:1951	physiological or activated conditions	1915:1951	physiological or activated conditions	1915:1951	CONCLUSION mTAP showed higher cytotoxicity than CMC and mCMC and the effect of topic antimicrobials might differ when tested against apical papilla cells under physiological or activated conditions.
30810637	4	38	with	test	880:883	arg1	µg/mL					896:900	1,000 µg/mL	890:900	1,000 µg/mL of each substance	890:918	Further, cells were primed with 1 µg/mL of Enterococcus faecalis LTA for 7 days prior to the viability test with 1,000 µg/mL of each substance.
30810637	0	39	theme	intracanal	16:25	arg1	dressings					27:35	intracanal dressings	16:35	intracanal dressings	16:35	Cytotoxicity of intracanal dressings on apical papilla cells differ upon activation with E. faecalis LTA.
30810637	1	40	theme	acid	287:290	arg1	APC					327:329	APC	327:329	APC	327:329	OBJECTIVE The aim of this study was to investigate the cytotoxic effects of modified triple antibiotic paste and an experimental composition using calcium hydroxide on lipoteichoic acid (LTA)-primed apical papilla cells (APC).
30810637	1	40	theme	acid	287:290	arg1	cells					320:324	lipoteichoic acid (LTA)-primed apical papilla cells	274:324	lipoteichoic acid (LTA)-primed apical papilla cells (APC)	274:330	OBJECTIVE The aim of this study was to investigate the cytotoxic effects of modified triple antibiotic paste and an experimental composition using calcium hydroxide on lipoteichoic acid (LTA)-primed apical papilla cells (APC).
30810637	7	41	theme	cytotoxic	1140:1148	arg1	rates					1150:1154	the higher cytotoxic rates	1129:1154	the higher cytotoxic rates	1129:1154	RESULTS In the first assay, the higher cytotoxic rates were reached by mTAP for all experimental periods.
30810637	1	42	theme	modified	182:189	arg1	paste					209:213	modified triple antibiotic paste	182:213	modified triple antibiotic paste	182:213	OBJECTIVE The aim of this study was to investigate the cytotoxic effects of modified triple antibiotic paste and an experimental composition using calcium hydroxide on lipoteichoic acid (LTA)-primed apical papilla cells (APC).
30810637	0	43	theme	apical	40:45	arg1	cells					55:59	apical papilla cells	40:59	apical papilla cells	40:59	Cytotoxicity of intracanal dressings on apical papilla cells differ upon activation with E. faecalis LTA.
30810637	10	44	theme	LTA-primed	1437:1446	arg1	cells					1448:1452	LTA-primed cells	1437:1452	LTA-primed cells	1437:1452	In the second assay, when considering the condition with medium only, LTA-primed cells significantly proliferated in comparison to LTA-untreated ones.
30810637	1	45	theme	triple	191:196	arg1	paste					209:213	modified triple antibiotic paste	182:213	modified triple antibiotic paste	182:213	OBJECTIVE The aim of this study was to investigate the cytotoxic effects of modified triple antibiotic paste and an experimental composition using calcium hydroxide on lipoteichoic acid (LTA)-primed apical papilla cells (APC).
30810637	11	46	theme	similar	1555:1561	arg1	cytotoxicity					1563:1574	similar cytotoxicity	1555:1574	similar cytotoxicity	1555:1574	At this context, mTAP and CMC showed similar cytotoxicity than the observed for LTA-untreated cells, while mCMC was shown cytotoxic at 7 days only for LTA-primed APC.
30810637	13	47	theme	apical	1888:1893	arg1	cells					1903:1907	apical papilla cells	1888:1907	apical papilla cells	1888:1907	CONCLUSION mTAP showed higher cytotoxicity than CMC and mCMC and the effect of topic antimicrobials might differ when tested against apical papilla cells under physiological or activated conditions.
30810637	2	48	theme	MTT	622:624	arg1	assay					626:630	MTT assay	622:630	MTT assay	622:630	MATERIAL AND METHODS Human APC were tested for in vitro cytotoxicity of modified Triple Antibiotic Paste (mTAP - Ciprofloxacin, Metronidazole and Cefaclor at 1:1:1) and of a paste of Ciprofloxacin, Metronidazole and Calcium hydroxide (CMC - 1:1:2) and modified CMC (mCMC - 2:2:1) by using MTT assay.
30810637	1	49	theme	antibiotic	198:207	arg1	paste					209:213	modified triple antibiotic paste	182:213	modified triple antibiotic paste	182:213	OBJECTIVE The aim of this study was to investigate the cytotoxic effects of modified triple antibiotic paste and an experimental composition using calcium hydroxide on lipoteichoic acid (LTA)-primed apical papilla cells (APC).
30810637	2	50	theme	Paste	432:436	arg1	cytotoxicity					389:400	in vitro cytotoxicity	380:400	in vitro cytotoxicity of modified Triple Antibiotic Paste (mTAP - Ciprofloxacin, Metronidazole and Cefaclor at 1:1:1) and of a paste of Ciprofloxacin, Metronidazole and Calcium hydroxide (CMC - 1:1:2) and modified CMC (mCMC - 2:2:1)	380:611	MATERIAL AND METHODS Human APC were tested for in vitro cytotoxicity of modified Triple Antibiotic Paste (mTAP - Ciprofloxacin, Metronidazole and Cefaclor at 1:1:1) and of a paste of Ciprofloxacin, Metronidazole and Calcium hydroxide (CMC - 1:1:2) and modified CMC (mCMC - 2:2:1) by using MTT assay.
30810637	3	51	from	¼	694:694	arg1	DMEM					670:673	DMEM	670:673	DMEM at 1,000 µg/mL and ¼ serially diluted before being kept in contact with cells for 1, 3, 5 and 7 days	670:774	The substances were reconstituted in DMEM at 1,000 µg/mL and ¼ serially diluted before being kept in contact with cells for 1, 3, 5 and 7 days.
30810637	5	52	theme	Statistical	921:931	arg1	analysis					933:940	Statistical analysis	921:940	Statistical analysis	921:940	Statistical analysis was performed using one-way analysis of variance (ANOVA) and two-way ANOVA respectively followed by Tukey's post-test.
30810637	6	53	theme	Significance	1061:1072	arg1	levels					1074:1079	Significance levels	1061:1079	Significance levels	1061:1079	Significance levels were set at p<0.05.
30810637	2	54	dep	MATERIAL	333:340	arg1	APC					360:362	Human APC	354:362	Human APC	354:362	MATERIAL AND METHODS Human APC were tested for in vitro cytotoxicity of modified Triple Antibiotic Paste (mTAP - Ciprofloxacin, Metronidazole and Cefaclor at 1:1:1) and of a paste of Ciprofloxacin, Metronidazole and Calcium hydroxide (CMC - 1:1:2) and modified CMC (mCMC - 2:2:1) by using MTT assay.
30810637	13	55	theme	higher	1778:1783	arg1	cytotoxicity					1785:1796	higher cytotoxicity	1778:1796	higher cytotoxicity	1778:1796	CONCLUSION mTAP showed higher cytotoxicity than CMC and mCMC and the effect of topic antimicrobials might differ when tested against apical papilla cells under physiological or activated conditions.
30810637	0	56	theme	papilla	47:53	arg1	cells					55:59	apical papilla cells	40:59	apical papilla cells	40:59	Cytotoxicity of intracanal dressings on apical papilla cells differ upon activation with E. faecalis LTA.
30810637	1	57	theme	-primed	297:303	arg1	APC					327:329	APC	327:329	APC	327:329	OBJECTIVE The aim of this study was to investigate the cytotoxic effects of modified triple antibiotic paste and an experimental composition using calcium hydroxide on lipoteichoic acid (LTA)-primed apical papilla cells (APC).
30810637	1	57	theme	-primed	297:303	arg1	cells					320:324	lipoteichoic acid (LTA)-primed apical papilla cells	274:324	lipoteichoic acid (LTA)-primed apical papilla cells (APC)	274:330	OBJECTIVE The aim of this study was to investigate the cytotoxic effects of modified triple antibiotic paste and an experimental composition using calcium hydroxide on lipoteichoic acid (LTA)-primed apical papilla cells (APC).
30810637	2	58	theme	Ciprofloxacin	516:528	arg1	paste					507:511	a paste	505:511	a paste of Ciprofloxacin, Metronidazole and Calcium hydroxide (CMC - 1:1:2) and modified CMC (mCMC - 2:2:1)	505:611	MATERIAL AND METHODS Human APC were tested for in vitro cytotoxicity of modified Triple Antibiotic Paste (mTAP - Ciprofloxacin, Metronidazole and Cefaclor at 1:1:1) and of a paste of Ciprofloxacin, Metronidazole and Calcium hydroxide (CMC - 1:1:2) and modified CMC (mCMC - 2:2:1) by using MTT assay.
30810637	13	59	theme	antimicrobials	1840:1853	arg1	effect					1824:1829	the effect	1820:1829	the effect of topic antimicrobials	1820:1853	CONCLUSION mTAP showed higher cytotoxicity than CMC and mCMC and the effect of topic antimicrobials might differ when tested against apical papilla cells under physiological or activated conditions.
30810637	2	60	dep	in	380:381	arg1	vitro					383:387	vitro	383:387	vitro	383:387	MATERIAL AND METHODS Human APC were tested for in vitro cytotoxicity of modified Triple Antibiotic Paste (mTAP - Ciprofloxacin, Metronidazole and Cefaclor at 1:1:1) and of a paste of Ciprofloxacin, Metronidazole and Calcium hydroxide (CMC - 1:1:2) and modified CMC (mCMC - 2:2:1) by using MTT assay.
30810637	7	61	theme	first	1116:1120	arg1	assay					1122:1126	the first assay	1112:1126	the first assay	1112:1126	RESULTS In the first assay, the higher cytotoxic rates were reached by mTAP for all experimental periods.
30810637	4	62	theme	substance	910:918	arg1	µg/mL					896:900	1,000 µg/mL	890:900	1,000 µg/mL of each substance	890:918	Further, cells were primed with 1 µg/mL of Enterococcus faecalis LTA for 7 days prior to the viability test with 1,000 µg/mL of each substance.
30810637	1	63	theme	apical	305:310	arg1	APC					327:329	APC	327:329	APC	327:329	OBJECTIVE The aim of this study was to investigate the cytotoxic effects of modified triple antibiotic paste and an experimental composition using calcium hydroxide on lipoteichoic acid (LTA)-primed apical papilla cells (APC).
30810637	1	63	theme	apical	305:310	arg1	cells					320:324	lipoteichoic acid (LTA)-primed apical papilla cells	274:324	lipoteichoic acid (LTA)-primed apical papilla cells (APC)	274:330	OBJECTIVE The aim of this study was to investigate the cytotoxic effects of modified triple antibiotic paste and an experimental composition using calcium hydroxide on lipoteichoic acid (LTA)-primed apical papilla cells (APC).
30810637	2	64	theme	modified	585:592	arg1	CMC					594:596	modified CMC	585:596	modified CMC (mCMC - 2:2:1)	585:611	MATERIAL AND METHODS Human APC were tested for in vitro cytotoxicity of modified Triple Antibiotic Paste (mTAP - Ciprofloxacin, Metronidazole and Cefaclor at 1:1:1) and of a paste of Ciprofloxacin, Metronidazole and Calcium hydroxide (CMC - 1:1:2) and modified CMC (mCMC - 2:2:1) by using MTT assay.
30810637	1	65	theme	paste	209:213	arg1	effects					171:177	the cytotoxic effects	157:177	the cytotoxic effects of modified triple antibiotic paste and an experimental composition using calcium hydroxide on lipoteichoic acid (LTA)-primed apical papilla cells (APC)	157:330	OBJECTIVE The aim of this study was to investigate the cytotoxic effects of modified triple antibiotic paste and an experimental composition using calcium hydroxide on lipoteichoic acid (LTA)-primed apical papilla cells (APC).
30810637	2	66	theme	paste	507:511	arg1	cytotoxicity					389:400	in vitro cytotoxicity	380:400	in vitro cytotoxicity of modified Triple Antibiotic Paste (mTAP - Ciprofloxacin, Metronidazole and Cefaclor at 1:1:1) and of a paste of Ciprofloxacin, Metronidazole and Calcium hydroxide (CMC - 1:1:2) and modified CMC (mCMC - 2:2:1)	380:611	MATERIAL AND METHODS Human APC were tested for in vitro cytotoxicity of modified Triple Antibiotic Paste (mTAP - Ciprofloxacin, Metronidazole and Cefaclor at 1:1:1) and of a paste of Ciprofloxacin, Metronidazole and Calcium hydroxide (CMC - 1:1:2) and modified CMC (mCMC - 2:2:1) by using MTT assay.
30810637	2	67	from	1:1:1	491:495	arg1	Cefaclor					479:486	Cefaclor	479:486	Cefaclor	479:486	MATERIAL AND METHODS Human APC were tested for in vitro cytotoxicity of modified Triple Antibiotic Paste (mTAP - Ciprofloxacin, Metronidazole and Cefaclor at 1:1:1) and of a paste of Ciprofloxacin, Metronidazole and Calcium hydroxide (CMC - 1:1:2) and modified CMC (mCMC - 2:2:1) by using MTT assay.
30810637	2	67	from	1:1:1	491:495	arg1	Metronidazole					461:473	Metronidazole	461:473	Metronidazole	461:473	MATERIAL AND METHODS Human APC were tested for in vitro cytotoxicity of modified Triple Antibiotic Paste (mTAP - Ciprofloxacin, Metronidazole and Cefaclor at 1:1:1) and of a paste of Ciprofloxacin, Metronidazole and Calcium hydroxide (CMC - 1:1:2) and modified CMC (mCMC - 2:2:1) by using MTT assay.
30810637	2	67	from	1:1:1	491:495	arg1	Ciprofloxacin					446:458	Ciprofloxacin	446:458	Ciprofloxacin	446:458	MATERIAL AND METHODS Human APC were tested for in vitro cytotoxicity of modified Triple Antibiotic Paste (mTAP - Ciprofloxacin, Metronidazole and Cefaclor at 1:1:1) and of a paste of Ciprofloxacin, Metronidazole and Calcium hydroxide (CMC - 1:1:2) and modified CMC (mCMC - 2:2:1) by using MTT assay.
30810637	7	68	dep	RESULTS	1101:1107	arg1	reached					1161:1167	reached	1161:1167	were reached by mTAP for all experimental periods	1156:1204	RESULTS In the first assay, the higher cytotoxic rates were reached by mTAP for all experimental periods.
30810637	8	69	theme	cell	1284:1287	arg1	viability					1289:1297	the cell viability	1280:1297	the cell viability	1280:1297	CMC was found toxic for APC at 5 and 7 days, whereas mCMC did not affect the cell viability.
30810637	3	70	with	contact	734:740	arg1	cells					747:751	cells	747:751	cells	747:751	The substances were reconstituted in DMEM at 1,000 µg/mL and ¼ serially diluted before being kept in contact with cells for 1, 3, 5 and 7 days.
30810637	5	71	theme	variance	982:989	arg1	analysis					970:977	one-way analysis	962:977	one-way analysis of variance (ANOVA)	962:997	Statistical analysis was performed using one-way analysis of variance (ANOVA) and two-way ANOVA respectively followed by Tukey's post-test.
30810637	5	71	theme	variance	982:989	arg1	ANOVA					1011:1015	two-way ANOVA	1003:1015	two-way ANOVA respectively followed by Tukey's post-test	1003:1058	Statistical analysis was performed using one-way analysis of variance (ANOVA) and two-way ANOVA respectively followed by Tukey's post-test.
30810637	1	72	from	effects	171:177	arg1	APC					327:329	APC	327:329	APC	327:329	OBJECTIVE The aim of this study was to investigate the cytotoxic effects of modified triple antibiotic paste and an experimental composition using calcium hydroxide on lipoteichoic acid (LTA)-primed apical papilla cells (APC).
30810637	1	72	from	effects	171:177	arg1	cells					320:324	lipoteichoic acid (LTA)-primed apical papilla cells	274:324	lipoteichoic acid (LTA)-primed apical papilla cells (APC)	274:330	OBJECTIVE The aim of this study was to investigate the cytotoxic effects of modified triple antibiotic paste and an experimental composition using calcium hydroxide on lipoteichoic acid (LTA)-primed apical papilla cells (APC).
30810637	13	73	theme	physiological	1915:1927	arg1	conditions					1942:1951	physiological or activated conditions	1915:1951	physiological or activated conditions	1915:1951	CONCLUSION mTAP showed higher cytotoxicity than CMC and mCMC and the effect of topic antimicrobials might differ when tested against apical papilla cells under physiological or activated conditions.
30810637	11	74	theme	LTA-untreated	1598:1610	arg1	cells					1612:1616	LTA-untreated cells	1598:1616	LTA-untreated cells	1598:1616	At this context, mTAP and CMC showed similar cytotoxicity than the observed for LTA-untreated cells, while mCMC was shown cytotoxic at 7 days only for LTA-primed APC.
30810637	4	75	theme	prior	857:861	arg1	days					852:855	7 days	850:855	7 days prior to the viability test with 1,000 µg/mL of each substance	850:918	Further, cells were primed with 1 µg/mL of Enterococcus faecalis LTA for 7 days prior to the viability test with 1,000 µg/mL of each substance.
30810637	1	76	theme	papilla	312:318	arg1	APC					327:329	APC	327:329	APC	327:329	OBJECTIVE The aim of this study was to investigate the cytotoxic effects of modified triple antibiotic paste and an experimental composition using calcium hydroxide on lipoteichoic acid (LTA)-primed apical papilla cells (APC).
30810637	1	76	theme	papilla	312:318	arg1	cells					320:324	lipoteichoic acid (LTA)-primed apical papilla cells	274:324	lipoteichoic acid (LTA)-primed apical papilla cells (APC)	274:330	OBJECTIVE The aim of this study was to investigate the cytotoxic effects of modified triple antibiotic paste and an experimental composition using calcium hydroxide on lipoteichoic acid (LTA)-primed apical papilla cells (APC).
30810637	4	77	theme	viability	870:878	arg1	test					880:883	the viability test	866:883	the viability test with 1,000 µg/mL of each substance	866:918	Further, cells were primed with 1 µg/mL of Enterococcus faecalis LTA for 7 days prior to the viability test with 1,000 µg/mL of each substance.
30810637	13	78	theme	CONCLUSION	1755:1764	arg1	mTAP					1766:1769	CONCLUSION mTAP	1755:1769	CONCLUSION mTAP	1755:1769	CONCLUSION mTAP showed higher cytotoxicity than CMC and mCMC and the effect of topic antimicrobials might differ when tested against apical papilla cells under physiological or activated conditions.
30810637	1	79	theme	study	132:136	arg1	aim					120:122	The aim	116:122	The aim of this study	116:136	OBJECTIVE The aim of this study was to investigate the cytotoxic effects of modified triple antibiotic paste and an experimental composition using calcium hydroxide on lipoteichoic acid (LTA)-primed apical papilla cells (APC).
30810637	1	80	theme	experimental	222:233	arg1	composition					235:245	an experimental composition	219:245	an experimental composition using calcium hydroxide	219:269	OBJECTIVE The aim of this study was to investigate the cytotoxic effects of modified triple antibiotic paste and an experimental composition using calcium hydroxide on lipoteichoic acid (LTA)-primed apical papilla cells (APC).
29384363	5	0	theme	chain	623:627	arg1	oxidation					593:601	the rapid, chemoselective oxidation	567:601	the rapid, chemoselective oxidation of sialic acid side chain in common ganglioside core structures using NaIO4 followed by ligation with a carbonyl-reactive isobaric tandem mass tag (TMT) reagent and subsequent RPLC-MS/MS analysis	567:797	Specifically, we carried out the rapid, chemoselective oxidation of sialic acid side chain in common ganglioside core structures using NaIO4 followed by ligation with a carbonyl-reactive isobaric tandem mass tag (TMT) reagent and subsequent RPLC-MS/MS analysis.
29384363	5	1	theme	isobaric	725:732	arg1	reagent					756:762	a carbonyl-reactive isobaric tandem mass tag (TMT) reagent	705:762	a carbonyl-reactive isobaric tandem mass tag (TMT) reagent	705:762	Specifically, we carried out the rapid, chemoselective oxidation of sialic acid side chain in common ganglioside core structures using NaIO4 followed by ligation with a carbonyl-reactive isobaric tandem mass tag (TMT) reagent and subsequent RPLC-MS/MS analysis.
29384363	2	2	theme	molecular	340:348	arg1	species					350:356	intact molecular species	333:356	intact molecular species	333:356	Both the glycan and lipid structures influence their biological function and thus necessitate their determination as intact molecular species.
29384363	2	2	theme	molecular	340:348	arg1	determination					316:328	their determination	310:328	their determination	310:328	Both the glycan and lipid structures influence their biological function and thus necessitate their determination as intact molecular species.
29384363	8	3	theme	compositions	1239:1250	arg1	identification					1204:1217	identification	1204:1217	simultaneous quantification of up to six samples as well as identification of glycan and lipid compositions	1144:1250	This facile approach enabled simultaneous quantification of up to six samples as well as identification of glycan and lipid compositions in a single injection.
29384363	8	3	theme	compositions	1239:1250	arg1	quantification					1157:1170	simultaneous quantification	1144:1170	simultaneous quantification of up to six samples as well as identification of glycan and lipid compositions	1144:1250	This facile approach enabled simultaneous quantification of up to six samples as well as identification of glycan and lipid compositions in a single injection.
29384363	10	4	theme	great	1471:1475	arg1	promise					1477:1483	the great promise	1467:1483	the great promise	1467:1483	This showcased the great promise and feasibility of this strategy for high-throughput analysis of intact gangliosides in biological extracts.
29384363	9	5	from	ratios	1361:1366	arg1	pooled					1345:1350	pooled	1345:1350	pooled	1345:1350	As a proof-of-concept, using porcine brain total ganglioside extracts pooled at known ratios, we obtained overall sample-to-sample precision of <12% RSD and mean error of <10%.
29384363	5	6	with	analysis	790:797	arg1	reagent					756:762	a carbonyl-reactive isobaric tandem mass tag (TMT) reagent	705:762	a carbonyl-reactive isobaric tandem mass tag (TMT) reagent	705:762	Specifically, we carried out the rapid, chemoselective oxidation of sialic acid side chain in common ganglioside core structures using NaIO4 followed by ligation with a carbonyl-reactive isobaric tandem mass tag (TMT) reagent and subsequent RPLC-MS/MS analysis.
29384363	5	7	dep	rapid	571:575	arg1	chemoselective					578:591	chemoselective	578:591	chemoselective	578:591	Specifically, we carried out the rapid, chemoselective oxidation of sialic acid side chain in common ganglioside core structures using NaIO4 followed by ligation with a carbonyl-reactive isobaric tandem mass tag (TMT) reagent and subsequent RPLC-MS/MS analysis.
29384363	9	8	theme	porcine	1304:1310	arg1	extracts					1336:1343	porcine brain total ganglioside extracts	1304:1343	porcine brain total ganglioside extracts pooled at known ratios	1304:1366	As a proof-of-concept, using porcine brain total ganglioside extracts pooled at known ratios, we obtained overall sample-to-sample precision of <12% RSD and mean error of <10%.
29384363	5	9	theme	tag	746:748	arg1	reagent					756:762	a carbonyl-reactive isobaric tandem mass tag (TMT) reagent	705:762	a carbonyl-reactive isobaric tandem mass tag (TMT) reagent	705:762	Specifically, we carried out the rapid, chemoselective oxidation of sialic acid side chain in common ganglioside core structures using NaIO4 followed by ligation with a carbonyl-reactive isobaric tandem mass tag (TMT) reagent and subsequent RPLC-MS/MS analysis.
29384363	5	10	theme	mass	741:744	arg1	reagent					756:762	a carbonyl-reactive isobaric tandem mass tag (TMT) reagent	705:762	a carbonyl-reactive isobaric tandem mass tag (TMT) reagent	705:762	Specifically, we carried out the rapid, chemoselective oxidation of sialic acid side chain in common ganglioside core structures using NaIO4 followed by ligation with a carbonyl-reactive isobaric tandem mass tag (TMT) reagent and subsequent RPLC-MS/MS analysis.
29384363	9	11	theme	total	1318:1322	arg1	extracts					1336:1343	porcine brain total ganglioside extracts	1304:1343	porcine brain total ganglioside extracts pooled at known ratios	1304:1366	As a proof-of-concept, using porcine brain total ganglioside extracts pooled at known ratios, we obtained overall sample-to-sample precision of <12% RSD and mean error of <10%.
29384363	5	12	theme	sialic	606:611	arg1	chain					623:627	sialic acid side chain	606:627	sialic acid side chain	606:627	Specifically, we carried out the rapid, chemoselective oxidation of sialic acid side chain in common ganglioside core structures using NaIO4 followed by ligation with a carbonyl-reactive isobaric tandem mass tag (TMT) reagent and subsequent RPLC-MS/MS analysis.
29384363	7	13	theme	reporter	1100:1107	arg1	ions					1109:1112	the glycan, lipid, and TMT reporter ions	1073:1112	ions	1109:1112	Fragmentation of the resulting [M + 2H]2+ ions of TMT-labeled gangliosides provided information-rich spectra containing fragments from the glycan, lipid, and TMT reporter ions.
29384363	1	14	theme	essential	176:184	arg1	role					186:189	essential role	176:189	essential role	176:189	Gangliosides are sialic acid-containing glycosphingolipids recognized to play essential role in biological processes.
29384363	3	15	theme	multiplexed	380:390	arg1	method					392:397	no multiplexed method	377:397	no multiplexed method for intact gangliosides	377:421	To our knowledge, no multiplexed method for intact gangliosides currently exists.
29384363	0	16	theme	Quantitative	75:86	arg1	Analysis					88:95	Multiplexed LC-MS/MS-Based Quantitative Analysis	48:95	Multiplexed LC-MS/MS-Based Quantitative Analysis	48:95	Isobaric Labeling of Intact Gangliosides toward Multiplexed LC-MS/MS-Based Quantitative Analysis.
29384363	5	17	theme	ganglioside	639:649	arg1	structures					656:665	common ganglioside core structures	632:665	common ganglioside core structures using NaIO4 followed by ligation with a carbonyl-reactive isobaric tandem mass tag (TMT) reagent and subsequent RPLC-MS/MS analysis	632:797	Specifically, we carried out the rapid, chemoselective oxidation of sialic acid side chain in common ganglioside core structures using NaIO4 followed by ligation with a carbonyl-reactive isobaric tandem mass tag (TMT) reagent and subsequent RPLC-MS/MS analysis.
29384363	5	18	theme	RPLC-MS/MS	779:788	arg1	analysis					790:797	subsequent RPLC-MS/MS analysis	768:797	subsequent RPLC-MS/MS analysis	768:797	Specifically, we carried out the rapid, chemoselective oxidation of sialic acid side chain in common ganglioside core structures using NaIO4 followed by ligation with a carbonyl-reactive isobaric tandem mass tag (TMT) reagent and subsequent RPLC-MS/MS analysis.
29384363	4	19	theme	intact	517:522	arg1	gangliosides					524:535	intact gangliosides	517:535	intact gangliosides	517:535	In this paper, we aimed to demonstrate an approach for isobaric labeling of intact gangliosides.
29384363	10	20	theme	high-throughput	1522:1536	arg1	analysis					1538:1545	high-throughput analysis	1522:1545	high-throughput analysis of intact gangliosides	1522:1568	This showcased the great promise and feasibility of this strategy for high-throughput analysis of intact gangliosides in biological extracts.
29384363	9	21	theme	overall	1381:1387	arg1	precision					1406:1414	overall sample-to-sample precision	1381:1414	overall sample-to-sample precision of <12% RSD and mean error of <10%	1381:1449	As a proof-of-concept, using porcine brain total ganglioside extracts pooled at known ratios, we obtained overall sample-to-sample precision of <12% RSD and mean error of <10%.
29384363	9	21	theme	overall	1381:1387	arg1	proof-of-concept					1280:1295	a proof-of-concept	1278:1295	a proof-of-concept	1278:1295	As a proof-of-concept, using porcine brain total ganglioside extracts pooled at known ratios, we obtained overall sample-to-sample precision of <12% RSD and mean error of <10%.
29384363	5	22	theme	side	618:621	arg1	chain					623:627	sialic acid side chain	606:627	sialic acid side chain	606:627	Specifically, we carried out the rapid, chemoselective oxidation of sialic acid side chain in common ganglioside core structures using NaIO4 followed by ligation with a carbonyl-reactive isobaric tandem mass tag (TMT) reagent and subsequent RPLC-MS/MS analysis.
29384363	10	23	theme	strategy	1509:1516	arg1	feasibility					1489:1499	feasibility	1489:1499	feasibility	1489:1499	This showcased the great promise and feasibility of this strategy for high-throughput analysis of intact gangliosides in biological extracts.
29384363	10	23	theme	strategy	1509:1516	arg1	promise					1477:1483	the great promise	1467:1483	the great promise	1467:1483	This showcased the great promise and feasibility of this strategy for high-throughput analysis of intact gangliosides in biological extracts.
29384363	0	24	theme	Isobaric	0:7	arg1	Labeling					9:16	Isobaric Labeling	0:16	Isobaric Labeling of Intact Gangliosides toward Multiplexed LC-MS/MS-Based Quantitative Analysis.	0:96	Isobaric Labeling of Intact Gangliosides toward Multiplexed LC-MS/MS-Based Quantitative Analysis.
29384363	7	25	theme	ions	980:983	arg1	Fragmentation					938:950	Fragmentation	938:950	Fragmentation of the resulting [M + 2H]2+ ions of TMT-labeled gangliosides	938:1011	Fragmentation of the resulting [M + 2H]2+ ions of TMT-labeled gangliosides provided information-rich spectra containing fragments from the glycan, lipid, and TMT reporter ions.
29384363	1	26	theme	sialic	115:120	arg1	Gangliosides					98:109	Gangliosides	98:109	Gangliosides	98:109	Gangliosides are sialic acid-containing glycosphingolipids recognized to play essential role in biological processes.
29384363	1	26	theme	sialic	115:120	arg1	glycosphingolipids					138:155	sialic acid-containing glycosphingolipids	115:155	sialic acid-containing glycosphingolipids recognized to play essential role in biological processes	115:213	Gangliosides are sialic acid-containing glycosphingolipids recognized to play essential role in biological processes.
29384363	10	27	theme	gangliosides	1557:1568	arg1	analysis					1538:1545	high-throughput analysis	1522:1545	high-throughput analysis of intact gangliosides	1522:1568	This showcased the great promise and feasibility of this strategy for high-throughput analysis of intact gangliosides in biological extracts.
29384363	5	28	theme	rapid	571:575	arg1	oxidation					593:601	the rapid, chemoselective oxidation	567:601	the rapid, chemoselective oxidation of sialic acid side chain in common ganglioside core structures using NaIO4 followed by ligation with a carbonyl-reactive isobaric tandem mass tag (TMT) reagent and subsequent RPLC-MS/MS analysis	567:797	Specifically, we carried out the rapid, chemoselective oxidation of sialic acid side chain in common ganglioside core structures using NaIO4 followed by ligation with a carbonyl-reactive isobaric tandem mass tag (TMT) reagent and subsequent RPLC-MS/MS analysis.
29384363	9	29	theme	<12	1419:1421	arg1	%					1422:1422	%	1422:1422	%	1422:1422	As a proof-of-concept, using porcine brain total ganglioside extracts pooled at known ratios, we obtained overall sample-to-sample precision of <12% RSD and mean error of <10%.
29384363	8	30	theme	simultaneous	1144:1155	arg1	quantification					1157:1170	simultaneous quantification	1144:1170	simultaneous quantification of up to six samples as well as identification of glycan and lipid compositions	1144:1250	This facile approach enabled simultaneous quantification of up to six samples as well as identification of glycan and lipid compositions in a single injection.
29384363	0	31	theme	Gangliosides	28:39	arg1	Labeling					9:16	Isobaric Labeling	0:16	Isobaric Labeling of Intact Gangliosides toward Multiplexed LC-MS/MS-Based Quantitative Analysis.	0:96	Isobaric Labeling of Intact Gangliosides toward Multiplexed LC-MS/MS-Based Quantitative Analysis.
29384363	9	32	theme	RSD	1424:1426	arg1	precision					1406:1414	overall sample-to-sample precision	1381:1414	overall sample-to-sample precision of <12% RSD and mean error of <10%	1381:1449	As a proof-of-concept, using porcine brain total ganglioside extracts pooled at known ratios, we obtained overall sample-to-sample precision of <12% RSD and mean error of <10%.
29384363	9	32	theme	RSD	1424:1426	arg1	proof-of-concept					1280:1295	a proof-of-concept	1278:1295	a proof-of-concept	1278:1295	As a proof-of-concept, using porcine brain total ganglioside extracts pooled at known ratios, we obtained overall sample-to-sample precision of <12% RSD and mean error of <10%.
29384363	0	33	theme	Multiplexed	48:58	arg1	Analysis					88:95	Multiplexed LC-MS/MS-Based Quantitative Analysis	48:95	Multiplexed LC-MS/MS-Based Quantitative Analysis	48:95	Isobaric Labeling of Intact Gangliosides toward Multiplexed LC-MS/MS-Based Quantitative Analysis.
29384363	9	34	theme	mean	1432:1435	arg1	error					1437:1441	mean error	1432:1441	mean error of <10%	1432:1449	As a proof-of-concept, using porcine brain total ganglioside extracts pooled at known ratios, we obtained overall sample-to-sample precision of <12% RSD and mean error of <10%.
29384363	5	35	with	ligation	691:698	arg1	reagent					756:762	a carbonyl-reactive isobaric tandem mass tag (TMT) reagent	705:762	a carbonyl-reactive isobaric tandem mass tag (TMT) reagent	705:762	Specifically, we carried out the rapid, chemoselective oxidation of sialic acid side chain in common ganglioside core structures using NaIO4 followed by ligation with a carbonyl-reactive isobaric tandem mass tag (TMT) reagent and subsequent RPLC-MS/MS analysis.
29384363	10	36	theme	biological	1573:1582	arg1	extracts					1584:1591	biological extracts	1573:1591	biological extracts	1573:1591	This showcased the great promise and feasibility of this strategy for high-throughput analysis of intact gangliosides in biological extracts.
29384363	7	37	theme	2+	977:978	arg1	ions					980:983	the resulting [M + 2H]2+ ions	955:983	ions	980:983	Fragmentation of the resulting [M + 2H]2+ ions of TMT-labeled gangliosides provided information-rich spectra containing fragments from the glycan, lipid, and TMT reporter ions.
29384363	8	38	theme	samples	1185:1191	arg1	identification					1204:1217	identification	1204:1217	simultaneous quantification of up to six samples as well as identification of glycan and lipid compositions	1144:1250	This facile approach enabled simultaneous quantification of up to six samples as well as identification of glycan and lipid compositions in a single injection.
29384363	8	38	theme	samples	1185:1191	arg1	quantification					1157:1170	simultaneous quantification	1144:1170	simultaneous quantification of up to six samples as well as identification of glycan and lipid compositions	1144:1250	This facile approach enabled simultaneous quantification of up to six samples as well as identification of glycan and lipid compositions in a single injection.
29384363	6	39	theme	complex	886:892	arg1	gangliosides					894:905	complex gangliosides	886:905	complex gangliosides	886:905	Attachment of the isobaric label was observed to improve the ionization efficiency of complex gangliosides using electrospray ionization.
29384363	7	40	theme	2H	974:975	arg1	ions					980:983	the resulting [M + 2H]2+ ions	955:983	ions	980:983	Fragmentation of the resulting [M + 2H]2+ ions of TMT-labeled gangliosides provided information-rich spectra containing fragments from the glycan, lipid, and TMT reporter ions.
29384363	9	41	theme	%	1449:1449	arg1	RSD					1424:1426	<12% RSD	1419:1426	<12% RSD	1419:1426	As a proof-of-concept, using porcine brain total ganglioside extracts pooled at known ratios, we obtained overall sample-to-sample precision of <12% RSD and mean error of <10%.
29384363	9	41	theme	%	1449:1449	arg1	error					1437:1441	mean error	1432:1441	mean error of <10%	1432:1449	As a proof-of-concept, using porcine brain total ganglioside extracts pooled at known ratios, we obtained overall sample-to-sample precision of <12% RSD and mean error of <10%.
29384363	7	42	theme	[M	969:970	arg1	Fragmentation					938:950	Fragmentation	938:950	Fragmentation of the resulting [M + 2H]2+ ions of TMT-labeled gangliosides	938:1011	Fragmentation of the resulting [M + 2H]2+ ions of TMT-labeled gangliosides provided information-rich spectra containing fragments from the glycan, lipid, and TMT reporter ions.
29384363	6	43	dep	isobaric	818:825	arg1	label					827:831	label	827:831	label	827:831	Attachment of the isobaric label was observed to improve the ionization efficiency of complex gangliosides using electrospray ionization.
29384363	5	44	theme	carbonyl-reactive	707:723	arg1	reagent					756:762	a carbonyl-reactive isobaric tandem mass tag (TMT) reagent	705:762	a carbonyl-reactive isobaric tandem mass tag (TMT) reagent	705:762	Specifically, we carried out the rapid, chemoselective oxidation of sialic acid side chain in common ganglioside core structures using NaIO4 followed by ligation with a carbonyl-reactive isobaric tandem mass tag (TMT) reagent and subsequent RPLC-MS/MS analysis.
29384363	6	45	theme	ionization	861:870	arg1	efficiency					872:881	the ionization efficiency	857:881	the ionization efficiency of complex gangliosides	857:905	Attachment of the isobaric label was observed to improve the ionization efficiency of complex gangliosides using electrospray ionization.
29384363	8	46	theme	glycan	1222:1227	arg1	identification					1204:1217	identification	1204:1217	simultaneous quantification of up to six samples as well as identification of glycan and lipid compositions	1144:1250	This facile approach enabled simultaneous quantification of up to six samples as well as identification of glycan and lipid compositions in a single injection.
29384363	8	46	theme	glycan	1222:1227	arg1	quantification					1157:1170	simultaneous quantification	1144:1170	simultaneous quantification of up to six samples as well as identification of glycan and lipid compositions	1144:1250	This facile approach enabled simultaneous quantification of up to six samples as well as identification of glycan and lipid compositions in a single injection.
29384363	2	47	theme	intact	333:338	arg1	species					350:356	intact molecular species	333:356	intact molecular species	333:356	Both the glycan and lipid structures influence their biological function and thus necessitate their determination as intact molecular species.
29384363	2	47	theme	intact	333:338	arg1	determination					316:328	their determination	310:328	their determination	310:328	Both the glycan and lipid structures influence their biological function and thus necessitate their determination as intact molecular species.
29384363	8	48	theme	lipid	1233:1237	arg1	compositions					1239:1250	lipid compositions	1233:1250	lipid compositions	1233:1250	This facile approach enabled simultaneous quantification of up to six samples as well as identification of glycan and lipid compositions in a single injection.
29384363	8	49	dep	six	1181:1183	arg1	to					1178:1179	to	1178:1179	to	1178:1179	This facile approach enabled simultaneous quantification of up to six samples as well as identification of glycan and lipid compositions in a single injection.
29384363	5	50	theme	TMT	751:753	arg1	reagent					756:762	a carbonyl-reactive isobaric tandem mass tag (TMT) reagent	705:762	a carbonyl-reactive isobaric tandem mass tag (TMT) reagent	705:762	Specifically, we carried out the rapid, chemoselective oxidation of sialic acid side chain in common ganglioside core structures using NaIO4 followed by ligation with a carbonyl-reactive isobaric tandem mass tag (TMT) reagent and subsequent RPLC-MS/MS analysis.
29384363	8	51	theme	single	1257:1262	arg1	injection					1264:1272	a single injection	1255:1272	a single injection	1255:1272	This facile approach enabled simultaneous quantification of up to six samples as well as identification of glycan and lipid compositions in a single injection.
29384363	4	52	theme	isobaric	496:503	arg1	labeling					505:512	isobaric labeling	496:512	isobaric labeling of intact gangliosides	496:535	In this paper, we aimed to demonstrate an approach for isobaric labeling of intact gangliosides.
29384363	7	53	from	lipid	1085:1089	arg1	fragments					1058:1066	fragments	1058:1066	fragments from the glycan, lipid, and TMT reporter ions	1058:1112	Fragmentation of the resulting [M + 2H]2+ ions of TMT-labeled gangliosides provided information-rich spectra containing fragments from the glycan, lipid, and TMT reporter ions.
29384363	5	54	from	oxidation	593:601	arg1	structures					656:665	common ganglioside core structures	632:665	common ganglioside core structures using NaIO4 followed by ligation with a carbonyl-reactive isobaric tandem mass tag (TMT) reagent and subsequent RPLC-MS/MS analysis	632:797	Specifically, we carried out the rapid, chemoselective oxidation of sialic acid side chain in common ganglioside core structures using NaIO4 followed by ligation with a carbonyl-reactive isobaric tandem mass tag (TMT) reagent and subsequent RPLC-MS/MS analysis.
29384363	7	55	contain	containing	1047:1056	arg2	fragments					1058:1066	fragments	1058:1066	fragments from the glycan, lipid, and TMT reporter ions	1058:1112	Fragmentation of the resulting [M + 2H]2+ ions of TMT-labeled gangliosides provided information-rich spectra containing fragments from the glycan, lipid, and TMT reporter ions.
29384363	7	55	contain	containing	1047:1056	arg1	spectra					1039:1045	information-rich spectra	1022:1045	information-rich spectra containing fragments from the glycan, lipid, and TMT reporter ions	1022:1112	Fragmentation of the resulting [M + 2H]2+ ions of TMT-labeled gangliosides provided information-rich spectra containing fragments from the glycan, lipid, and TMT reporter ions.
29384363	5	56	theme	tandem	734:739	arg1	reagent					756:762	a carbonyl-reactive isobaric tandem mass tag (TMT) reagent	705:762	a carbonyl-reactive isobaric tandem mass tag (TMT) reagent	705:762	Specifically, we carried out the rapid, chemoselective oxidation of sialic acid side chain in common ganglioside core structures using NaIO4 followed by ligation with a carbonyl-reactive isobaric tandem mass tag (TMT) reagent and subsequent RPLC-MS/MS analysis.
29384363	9	57	theme	brain	1312:1316	arg1	extracts					1336:1343	porcine brain total ganglioside extracts	1304:1343	porcine brain total ganglioside extracts pooled at known ratios	1304:1366	As a proof-of-concept, using porcine brain total ganglioside extracts pooled at known ratios, we obtained overall sample-to-sample precision of <12% RSD and mean error of <10%.
29384363	2	58	theme	biological	269:278	arg1	function					280:287	their biological function	263:287	their biological function	263:287	Both the glycan and lipid structures influence their biological function and thus necessitate their determination as intact molecular species.
29384363	6	59	theme	gangliosides	894:905	arg1	efficiency					872:881	the ionization efficiency	857:881	the ionization efficiency of complex gangliosides	857:905	Attachment of the isobaric label was observed to improve the ionization efficiency of complex gangliosides using electrospray ionization.
29384363	7	60	theme	TMT	1096:1098	arg1	ions					1109:1112	the glycan, lipid, and TMT reporter ions	1073:1112	ions	1109:1112	Fragmentation of the resulting [M + 2H]2+ ions of TMT-labeled gangliosides provided information-rich spectra containing fragments from the glycan, lipid, and TMT reporter ions.
29384363	9	61	theme	ganglioside	1324:1334	arg1	extracts					1336:1343	porcine brain total ganglioside extracts	1304:1343	porcine brain total ganglioside extracts pooled at known ratios	1304:1366	As a proof-of-concept, using porcine brain total ganglioside extracts pooled at known ratios, we obtained overall sample-to-sample precision of <12% RSD and mean error of <10%.
29384363	5	62	theme	acid	613:616	arg1	chain					623:627	sialic acid side chain	606:627	sialic acid side chain	606:627	Specifically, we carried out the rapid, chemoselective oxidation of sialic acid side chain in common ganglioside core structures using NaIO4 followed by ligation with a carbonyl-reactive isobaric tandem mass tag (TMT) reagent and subsequent RPLC-MS/MS analysis.
29384363	9	63	theme	pooled	1345:1350	arg1	extracts					1336:1343	porcine brain total ganglioside extracts	1304:1343	porcine brain total ganglioside extracts pooled at known ratios	1304:1366	As a proof-of-concept, using porcine brain total ganglioside extracts pooled at known ratios, we obtained overall sample-to-sample precision of <12% RSD and mean error of <10%.
29384363	7	64	theme	glycan	1077:1082	arg1	lipid					1085:1089	the glycan, lipid, and TMT reporter ions	1073:1112	lipid	1085:1089	Fragmentation of the resulting [M + 2H]2+ ions of TMT-labeled gangliosides provided information-rich spectra containing fragments from the glycan, lipid, and TMT reporter ions.
29384363	9	65	theme	known	1355:1359	arg1	ratios					1361:1366	known ratios	1355:1366	known ratios	1355:1366	As a proof-of-concept, using porcine brain total ganglioside extracts pooled at known ratios, we obtained overall sample-to-sample precision of <12% RSD and mean error of <10%.
29384363	1	66	theme	biological	194:203	arg1	processes					205:213	biological processes	194:213	biological processes	194:213	Gangliosides are sialic acid-containing glycosphingolipids recognized to play essential role in biological processes.
29384363	5	67	theme	subsequent	768:777	arg1	analysis					790:797	subsequent RPLC-MS/MS analysis	768:797	subsequent RPLC-MS/MS analysis	768:797	Specifically, we carried out the rapid, chemoselective oxidation of sialic acid side chain in common ganglioside core structures using NaIO4 followed by ligation with a carbonyl-reactive isobaric tandem mass tag (TMT) reagent and subsequent RPLC-MS/MS analysis.
29384363	5	68	theme	core	651:654	arg1	structures					656:665	common ganglioside core structures	632:665	common ganglioside core structures using NaIO4 followed by ligation with a carbonyl-reactive isobaric tandem mass tag (TMT) reagent and subsequent RPLC-MS/MS analysis	632:797	Specifically, we carried out the rapid, chemoselective oxidation of sialic acid side chain in common ganglioside core structures using NaIO4 followed by ligation with a carbonyl-reactive isobaric tandem mass tag (TMT) reagent and subsequent RPLC-MS/MS analysis.
29384363	4	69	theme	gangliosides	524:535	arg1	labeling					505:512	isobaric labeling	496:512	isobaric labeling of intact gangliosides	496:535	In this paper, we aimed to demonstrate an approach for isobaric labeling of intact gangliosides.
29384363	5	70	contain	carried	555:561	arg1	we					552:553	we	552:553	we	552:553	Specifically, we carried out the rapid, chemoselective oxidation of sialic acid side chain in common ganglioside core structures using NaIO4 followed by ligation with a carbonyl-reactive isobaric tandem mass tag (TMT) reagent and subsequent RPLC-MS/MS analysis.
29384363	5	70	contain	carried	555:561	arg2	oxidation					593:601	the rapid, chemoselective oxidation	567:601	the rapid, chemoselective oxidation of sialic acid side chain in common ganglioside core structures using NaIO4 followed by ligation with a carbonyl-reactive isobaric tandem mass tag (TMT) reagent and subsequent RPLC-MS/MS analysis	567:797	Specifically, we carried out the rapid, chemoselective oxidation of sialic acid side chain in common ganglioside core structures using NaIO4 followed by ligation with a carbonyl-reactive isobaric tandem mass tag (TMT) reagent and subsequent RPLC-MS/MS analysis.
29384363	8	71	theme	facile	1120:1125	arg1	approach					1127:1134	This facile approach	1115:1134	This facile approach	1115:1134	This facile approach enabled simultaneous quantification of up to six samples as well as identification of glycan and lipid compositions in a single injection.
29384363	7	72	theme	information-rich	1022:1037	arg1	spectra					1039:1045	information-rich spectra	1022:1045	information-rich spectra containing fragments from the glycan, lipid, and TMT reporter ions	1022:1112	Fragmentation of the resulting [M + 2H]2+ ions of TMT-labeled gangliosides provided information-rich spectra containing fragments from the glycan, lipid, and TMT reporter ions.
29384363	3	73	theme	intact	403:408	arg1	gangliosides					410:421	intact gangliosides	403:421	intact gangliosides	403:421	To our knowledge, no multiplexed method for intact gangliosides currently exists.
29384363	9	74	theme	sample-to-sample	1389:1404	arg1	precision					1406:1414	overall sample-to-sample precision	1381:1414	overall sample-to-sample precision of <12% RSD and mean error of <10%	1381:1449	As a proof-of-concept, using porcine brain total ganglioside extracts pooled at known ratios, we obtained overall sample-to-sample precision of <12% RSD and mean error of <10%.
29384363	9	74	theme	sample-to-sample	1389:1404	arg1	proof-of-concept					1280:1295	a proof-of-concept	1278:1295	a proof-of-concept	1278:1295	As a proof-of-concept, using porcine brain total ganglioside extracts pooled at known ratios, we obtained overall sample-to-sample precision of <12% RSD and mean error of <10%.
29384363	7	75	theme	gangliosides	1000:1011	arg1	Fragmentation					938:950	Fragmentation	938:950	Fragmentation of the resulting [M + 2H]2+ ions of TMT-labeled gangliosides	938:1011	Fragmentation of the resulting [M + 2H]2+ ions of TMT-labeled gangliosides provided information-rich spectra containing fragments from the glycan, lipid, and TMT reporter ions.
29384363	0	76	theme	Intact	21:26	arg1	Gangliosides					28:39	Intact Gangliosides	21:39	Intact Gangliosides	21:39	Isobaric Labeling of Intact Gangliosides toward Multiplexed LC-MS/MS-Based Quantitative Analysis.
29384363	5	77	theme	common	632:637	arg1	structures					656:665	common ganglioside core structures	632:665	common ganglioside core structures using NaIO4 followed by ligation with a carbonyl-reactive isobaric tandem mass tag (TMT) reagent and subsequent RPLC-MS/MS analysis	632:797	Specifically, we carried out the rapid, chemoselective oxidation of sialic acid side chain in common ganglioside core structures using NaIO4 followed by ligation with a carbonyl-reactive isobaric tandem mass tag (TMT) reagent and subsequent RPLC-MS/MS analysis.
29384363	1	78	theme	acid-containing	122:136	arg1	Gangliosides					98:109	Gangliosides	98:109	Gangliosides	98:109	Gangliosides are sialic acid-containing glycosphingolipids recognized to play essential role in biological processes.
29384363	1	78	theme	acid-containing	122:136	arg1	glycosphingolipids					138:155	sialic acid-containing glycosphingolipids	115:155	sialic acid-containing glycosphingolipids recognized to play essential role in biological processes	115:213	Gangliosides are sialic acid-containing glycosphingolipids recognized to play essential role in biological processes.
29384363	10	79	theme	intact	1550:1555	arg1	gangliosides					1557:1568	intact gangliosides	1550:1568	intact gangliosides	1550:1568	This showcased the great promise and feasibility of this strategy for high-throughput analysis of intact gangliosides in biological extracts.
29384363	9	80	theme	%	1422:1422	arg1	RSD					1424:1426	<12% RSD	1419:1426	<12% RSD	1419:1426	As a proof-of-concept, using porcine brain total ganglioside extracts pooled at known ratios, we obtained overall sample-to-sample precision of <12% RSD and mean error of <10%.
29384363	6	81	theme	isobaric	818:825	arg1	Attachment					800:809	Attachment	800:809	Attachment of the isobaric label	800:831	Attachment of the isobaric label was observed to improve the ionization efficiency of complex gangliosides using electrospray ionization.
29384363	0	82	theme	LC-MS/MS-Based	60:73	arg1	Analysis					88:95	Multiplexed LC-MS/MS-Based Quantitative Analysis	48:95	Multiplexed LC-MS/MS-Based Quantitative Analysis	48:95	Isobaric Labeling of Intact Gangliosides toward Multiplexed LC-MS/MS-Based Quantitative Analysis.
29384363	6	83	theme	electrospray	913:924	arg1	ionization					926:935	electrospray ionization	913:935	electrospray ionization	913:935	Attachment of the isobaric label was observed to improve the ionization efficiency of complex gangliosides using electrospray ionization.
29384363	9	84	theme	error	1437:1441	arg1	precision					1406:1414	overall sample-to-sample precision	1381:1414	overall sample-to-sample precision of <12% RSD and mean error of <10%	1381:1449	As a proof-of-concept, using porcine brain total ganglioside extracts pooled at known ratios, we obtained overall sample-to-sample precision of <12% RSD and mean error of <10%.
29384363	9	84	theme	error	1437:1441	arg1	proof-of-concept					1280:1295	a proof-of-concept	1278:1295	a proof-of-concept	1278:1295	As a proof-of-concept, using porcine brain total ganglioside extracts pooled at known ratios, we obtained overall sample-to-sample precision of <12% RSD and mean error of <10%.
29384363	7	85	theme	TMT-labeled	988:998	arg1	gangliosides					1000:1011	TMT-labeled gangliosides	988:1011	TMT-labeled gangliosides	988:1011	Fragmentation of the resulting [M + 2H]2+ ions of TMT-labeled gangliosides provided information-rich spectra containing fragments from the glycan, lipid, and TMT reporter ions.
29384363	2	86	theme	lipid	236:240	arg1	structures					242:251	Both the glycan and lipid structures	216:251	structures	242:251	Both the glycan and lipid structures influence their biological function and thus necessitate their determination as intact molecular species.
29384363	7	87	from	ions	1109:1112	arg1	fragments					1058:1066	fragments	1058:1066	fragments from the glycan, lipid, and TMT reporter ions	1058:1112	Fragmentation of the resulting [M + 2H]2+ ions of TMT-labeled gangliosides provided information-rich spectra containing fragments from the glycan, lipid, and TMT reporter ions.
29384363	7	88	theme	resulting	959:967	arg1	[M					969:970	the resulting [M + 2H]2+ ions	955:983	[M	969:970	Fragmentation of the resulting [M + 2H]2+ ions of TMT-labeled gangliosides provided information-rich spectra containing fragments from the glycan, lipid, and TMT reporter ions.
30242844	4	0	theme	airway	459:464	arg1	microbiome					466:475	upper airway microbiome	453:475	upper airway microbiome of young adults and elderly	453:503	Here, the metagenome of upper airway microbiome of young adults and elderly was analyzed to identify their association with adult asthma.
30242844	10	1	theme	young	1478:1482	arg1	patients					1500:1507	young adult asthmatic patients	1478:1507	young adult asthmatic patients	1478:1507	Genes related to pentose phosphate pathway, lipopolysaccharide biosynthesis, flagella assembly, and bacterial chemotaxis-which may all be related to increased inflammation and colonization of pathogenic bacteria-were higher in young adult asthmatic patients.
30242844	6	2	theme	functional	705:714	arg1	genes					716:720	functional genes	705:720	functional genes	705:720	The compositions and functional genes of airway microbiome were analyzed by high-throughput sequencing.
30242844	9	3	from	reduction	1159:1167	arg1	inflammation					1172:1183	inflammation	1172:1183	inflammation	1172:1183	Genes related to lysine degradation, N-glycan biosynthesis, caprolactam degradation, and PPAR signaling pathway, which could be related to the reduction in inflammation and degradation of air pollutants, were higher in non-asthmatics.
30242844	9	3	from	reduction	1159:1167	arg1	degradation					1189:1199	degradation	1189:1199	degradation	1189:1199	Genes related to lysine degradation, N-glycan biosynthesis, caprolactam degradation, and PPAR signaling pathway, which could be related to the reduction in inflammation and degradation of air pollutants, were higher in non-asthmatics.
30242844	6	4	dep	compositions	688:699	arg1	The					684:686	The	684:686	The	684:686	The compositions and functional genes of airway microbiome were analyzed by high-throughput sequencing.
30242844	4	5	theme	young	480:484	arg1	adults					486:491	young adults	480:491	young adults	480:491	Here, the metagenome of upper airway microbiome of young adults and elderly was analyzed to identify their association with adult asthma.
30242844	10	6	theme	higher	1468:1473	arg1	bacteria-were					1454:1466	pathogenic bacteria-were	1443:1466	pathogenic bacteria-were higher in young adult asthmatic patients	1443:1507	Genes related to pentose phosphate pathway, lipopolysaccharide biosynthesis, flagella assembly, and bacterial chemotaxis-which may all be related to increased inflammation and colonization of pathogenic bacteria-were higher in young adult asthmatic patients.
30242844	8	7	theme	FEV1	981:984	arg1	%					985:985	FEV1%	981:985	FEV1% predicted in each age group	981:1013	Different bacteria were related to FEV1% predicted in each age group.
30242844	10	8	theme	pathogenic	1443:1452	arg1	bacteria-were					1454:1466	pathogenic bacteria-were	1443:1466	pathogenic bacteria-were higher in young adult asthmatic patients	1443:1507	Genes related to pentose phosphate pathway, lipopolysaccharide biosynthesis, flagella assembly, and bacterial chemotaxis-which may all be related to increased inflammation and colonization of pathogenic bacteria-were higher in young adult asthmatic patients.
30242844	11	9	theme	functional	1523:1532	arg1	genes					1534:1538	the functional genes	1519:1538	the functional genes of airway microbiome in elderly patients	1519:1579	However, the functional genes of airway microbiome in elderly patients were not significantly different according to asthma morbidity.
30242844	11	9	theme	functional	1523:1532	arg1	different					1604:1612	different	1604:1612	different	1604:1612	However, the functional genes of airway microbiome in elderly patients were not significantly different according to asthma morbidity.
30242844	7	10	theme	age	935:937	arg1	group					939:943	each age group	930:943	each age group	930:943	RESULTS The composition of microbiota differed between young adult and elderly, and it was different between asthmatics and non-asthmatics in each age group.
30242844	5	11	theme	elderly	632:638	arg1	patients					647:654	young adult and elderly asthma patients	616:654	young adult and elderly asthma patients	616:654	METHODS Nasopharyngeal swabs were collected from young adult and elderly asthma patients and non-asthmatic subjects.
30242844	11	12	theme	microbiome	1550:1559	arg1	genes					1534:1538	the functional genes	1519:1538	the functional genes of airway microbiome in elderly patients	1519:1579	However, the functional genes of airway microbiome in elderly patients were not significantly different according to asthma morbidity.
30242844	11	12	theme	microbiome	1550:1559	arg1	different					1604:1612	different	1604:1612	different	1604:1612	However, the functional genes of airway microbiome in elderly patients were not significantly different according to asthma morbidity.
30242844	4	13	with	association	536:546	arg1	asthma					559:564	adult asthma	553:564	adult asthma	553:564	Here, the metagenome of upper airway microbiome of young adults and elderly was analyzed to identify their association with adult asthma.
30242844	0	14	theme	young	87:91	arg1	adults					93:98	young adults	87:98	young adults	87:98	Different upper airway microbiome and their functional genes associated with asthma in young adults and elderly individuals.
30242844	5	15	theme	asthma	640:645	arg1	patients					647:654	young adult and elderly asthma patients	616:654	young adult and elderly asthma patients	616:654	METHODS Nasopharyngeal swabs were collected from young adult and elderly asthma patients and non-asthmatic subjects.
30242844	5	16	theme	Nasopharyngeal	575:588	arg1	swabs					590:594	Nasopharyngeal swabs	575:594	Nasopharyngeal swabs	575:594	METHODS Nasopharyngeal swabs were collected from young adult and elderly asthma patients and non-asthmatic subjects.
30242844	10	17	theme	lipopolysaccharide	1295:1312	arg1	biosynthesis					1314:1325	lipopolysaccharide biosynthesis	1295:1325	lipopolysaccharide biosynthesis	1295:1325	Genes related to pentose phosphate pathway, lipopolysaccharide biosynthesis, flagella assembly, and bacterial chemotaxis-which may all be related to increased inflammation and colonization of pathogenic bacteria-were higher in young adult asthmatic patients.
30242844	12	18	theme	various	1807:1813	arg1	roles					1815:1819	various roles	1807:1819	various roles	1807:1819	CONCLUSIONS These results suggest that the composition and function of upper airway microbiome could influence asthma pathogenesis, and the microbiome could play various roles depending on the age group.
30242844	4	19	theme	adult	553:557	arg1	asthma					559:564	adult asthma	553:564	adult asthma	553:564	Here, the metagenome of upper airway microbiome of young adults and elderly was analyzed to identify their association with adult asthma.
30242844	0	20	theme	elderly	104:110	arg1	individuals					112:122	elderly individuals	104:122	elderly individuals	104:122	Different upper airway microbiome and their functional genes associated with asthma in young adults and elderly individuals.
30242844	11	21	from	genes	1534:1538	arg1	patients					1572:1579	elderly patients	1564:1579	elderly patients	1564:1579	However, the functional genes of airway microbiome in elderly patients were not significantly different according to asthma morbidity.
30242844	11	22	theme	airway	1543:1548	arg1	microbiome					1550:1559	airway microbiome	1543:1559	airway microbiome	1543:1559	However, the functional genes of airway microbiome in elderly patients were not significantly different according to asthma morbidity.
30242844	7	23	from	different	879:887	arg1	group					939:943	each age group	930:943	each age group	930:943	RESULTS The composition of microbiota differed between young adult and elderly, and it was different between asthmatics and non-asthmatics in each age group.
30242844	10	24	theme	phosphate	1276:1284	arg1	pathway					1286:1292	pentose phosphate pathway	1268:1292	pentose phosphate pathway	1268:1292	Genes related to pentose phosphate pathway, lipopolysaccharide biosynthesis, flagella assembly, and bacterial chemotaxis-which may all be related to increased inflammation and colonization of pathogenic bacteria-were higher in young adult asthmatic patients.
30242844	4	25	theme	upper	453:457	arg1	microbiome					466:475	upper airway microbiome	453:475	upper airway microbiome of young adults and elderly	453:503	Here, the metagenome of upper airway microbiome of young adults and elderly was analyzed to identify their association with adult asthma.
30242844	10	26	theme	asthmatic	1490:1498	arg1	patients					1500:1507	young adult asthmatic patients	1478:1507	young adult asthmatic patients	1478:1507	Genes related to pentose phosphate pathway, lipopolysaccharide biosynthesis, flagella assembly, and bacterial chemotaxis-which may all be related to increased inflammation and colonization of pathogenic bacteria-were higher in young adult asthmatic patients.
30242844	9	27	theme	caprolactam	1076:1086	arg1	degradation					1088:1098	caprolactam degradation	1076:1098	caprolactam degradation	1076:1098	Genes related to lysine degradation, N-glycan biosynthesis, caprolactam degradation, and PPAR signaling pathway, which could be related to the reduction in inflammation and degradation of air pollutants, were higher in non-asthmatics.
30242844	0	28	theme	upper	10:14	arg1	microbiome					23:32	Different upper airway microbiome	0:32	Different upper airway microbiome	0:32	Different upper airway microbiome and their functional genes associated with asthma in young adults and elderly individuals.
30242844	7	29	dep	RESULTS	788:794	arg1	different					879:887	different	879:887	different	879:887	RESULTS The composition of microbiota differed between young adult and elderly, and it was different between asthmatics and non-asthmatics in each age group.
30242844	7	29	dep	RESULTS	788:794	arg1	differed					826:833	differed	826:833	differed between young adult and elderly	826:865	RESULTS The composition of microbiota differed between young adult and elderly, and it was different between asthmatics and non-asthmatics in each age group.
30242844	10	30	theme	adult	1484:1488	arg1	patients					1500:1507	young adult asthmatic patients	1478:1507	young adult asthmatic patients	1478:1507	Genes related to pentose phosphate pathway, lipopolysaccharide biosynthesis, flagella assembly, and bacterial chemotaxis-which may all be related to increased inflammation and colonization of pathogenic bacteria-were higher in young adult asthmatic patients.
30242844	1	31	from	Microbes	136:143	arg1	airway					152:157	the airway	148:157	the airway	148:157	BACKGROUND Microbes in the airway have been shown to be associated with the pathogenesis of asthma.
30242844	12	32	dep	CONCLUSIONS	1645:1655	arg1	suggest					1671:1677	suggest	1671:1677	suggest that the composition and function of upper airway microbiome could influence asthma pathogenesis, and the microbiome could play various roles depending on the age group.	1671:1847	CONCLUSIONS These results suggest that the composition and function of upper airway microbiome could influence asthma pathogenesis, and the microbiome could play various roles depending on the age group.
30242844	0	33	theme	Different	0:8	arg1	microbiome					23:32	Different upper airway microbiome	0:32	Different upper airway microbiome	0:32	Different upper airway microbiome and their functional genes associated with asthma in young adults and elderly individuals.
30242844	11	34	theme	elderly	1564:1570	arg1	patients					1572:1579	elderly patients	1564:1579	elderly patients	1564:1579	However, the functional genes of airway microbiome in elderly patients were not significantly different according to asthma morbidity.
30242844	5	35	theme	young	616:620	arg1	patients					647:654	young adult and elderly asthma patients	616:654	young adult and elderly asthma patients	616:654	METHODS Nasopharyngeal swabs were collected from young adult and elderly asthma patients and non-asthmatic subjects.
30242844	9	36	theme	air	1204:1206	arg1	pollutants					1208:1217	air pollutants	1204:1217	air pollutants	1204:1217	Genes related to lysine degradation, N-glycan biosynthesis, caprolactam degradation, and PPAR signaling pathway, which could be related to the reduction in inflammation and degradation of air pollutants, were higher in non-asthmatics.
30242844	2	37	theme	microbiome	298:307	arg1	dysbiosis					268:276	the dysbiosis	264:276	the dysbiosis of the lower airway microbiome	264:307	The upper airway microbiome influences the dysbiosis of the lower airway microbiome.
30242844	10	38	theme	bacterial	1351:1359	arg1	chemotaxis-which					1361:1376	bacterial chemotaxis-which	1351:1376	bacterial chemotaxis-which	1351:1376	Genes related to pentose phosphate pathway, lipopolysaccharide biosynthesis, flagella assembly, and bacterial chemotaxis-which may all be related to increased inflammation and colonization of pathogenic bacteria-were higher in young adult asthmatic patients.
30242844	3	39	theme	upper	345:349	arg1	microbiome					358:367	upper airway microbiome	345:367	upper airway microbiome	345:367	However, to date, the influence of upper airway microbiome for adult and elderly asthma has not been fully elucidated.
30242844	7	40	theme	microbiota	815:824	arg1	composition					800:810	The composition	796:810	The composition of microbiota	796:824	RESULTS The composition of microbiota differed between young adult and elderly, and it was different between asthmatics and non-asthmatics in each age group.
30242844	6	41	theme	high-throughput	760:774	arg1	sequencing					776:785	high-throughput sequencing	760:785	high-throughput sequencing	760:785	The compositions and functional genes of airway microbiome were analyzed by high-throughput sequencing.
30242844	2	42	theme	airway	291:296	arg1	microbiome					298:307	the lower airway microbiome	281:307	the lower airway microbiome	281:307	The upper airway microbiome influences the dysbiosis of the lower airway microbiome.
30242844	10	43	theme	increased	1400:1408	arg1	inflammation					1410:1421	increased inflammation	1400:1421	increased inflammation	1400:1421	Genes related to pentose phosphate pathway, lipopolysaccharide biosynthesis, flagella assembly, and bacterial chemotaxis-which may all be related to increased inflammation and colonization of pathogenic bacteria-were higher in young adult asthmatic patients.
30242844	3	44	theme	airway	351:356	arg1	microbiome					358:367	upper airway microbiome	345:367	upper airway microbiome	345:367	However, to date, the influence of upper airway microbiome for adult and elderly asthma has not been fully elucidated.
30242844	2	45	theme	lower	285:289	arg1	microbiome					298:307	the lower airway microbiome	281:307	the lower airway microbiome	281:307	The upper airway microbiome influences the dysbiosis of the lower airway microbiome.
30242844	9	46	theme	PPAR	1105:1108	arg1	pathway					1120:1126	PPAR signaling pathway	1105:1126	PPAR signaling pathway	1105:1126	Genes related to lysine degradation, N-glycan biosynthesis, caprolactam degradation, and PPAR signaling pathway, which could be related to the reduction in inflammation and degradation of air pollutants, were higher in non-asthmatics.
30242844	3	47	theme	elderly	383:389	arg1	asthma					391:396	adult and elderly asthma	373:396	adult and elderly asthma	373:396	However, to date, the influence of upper airway microbiome for adult and elderly asthma has not been fully elucidated.
30242844	7	48	from	group	939:943	arg1	different					879:887	different	879:887	different	879:887	RESULTS The composition of microbiota differed between young adult and elderly, and it was different between asthmatics and non-asthmatics in each age group.
30242844	12	49	theme	microbiome	1729:1738	arg1	function					1704:1711	function	1704:1711	function	1704:1711	CONCLUSIONS These results suggest that the composition and function of upper airway microbiome could influence asthma pathogenesis, and the microbiome could play various roles depending on the age group.
30242844	12	49	theme	microbiome	1729:1738	arg1	composition					1688:1698	composition	1688:1698	composition	1688:1698	CONCLUSIONS These results suggest that the composition and function of upper airway microbiome could influence asthma pathogenesis, and the microbiome could play various roles depending on the age group.
30242844	9	50	theme	signaling	1110:1118	arg1	pathway					1120:1126	PPAR signaling pathway	1105:1126	PPAR signaling pathway	1105:1126	Genes related to lysine degradation, N-glycan biosynthesis, caprolactam degradation, and PPAR signaling pathway, which could be related to the reduction in inflammation and degradation of air pollutants, were higher in non-asthmatics.
30242844	0	51	theme	airway	16:21	arg1	microbiome					23:32	Different upper airway microbiome	0:32	Different upper airway microbiome	0:32	Different upper airway microbiome and their functional genes associated with asthma in young adults and elderly individuals.
30242844	5	52	dep	young	616:620	arg1	adult					622:626	adult	622:626	adult	622:626	METHODS Nasopharyngeal swabs were collected from young adult and elderly asthma patients and non-asthmatic subjects.
30242844	8	53	theme	age	1005:1007	arg1	group					1009:1013	each age group	1000:1013	each age group	1000:1013	Different bacteria were related to FEV1% predicted in each age group.
30242844	11	54	theme	asthma	1627:1632	arg1	morbidity					1634:1642	asthma morbidity	1627:1642	asthma morbidity	1627:1642	However, the functional genes of airway microbiome in elderly patients were not significantly different according to asthma morbidity.
30242844	10	55	theme	pentose	1268:1274	arg1	pathway					1286:1292	pentose phosphate pathway	1268:1292	pentose phosphate pathway	1268:1292	Genes related to pentose phosphate pathway, lipopolysaccharide biosynthesis, flagella assembly, and bacterial chemotaxis-which may all be related to increased inflammation and colonization of pathogenic bacteria-were higher in young adult asthmatic patients.
30242844	3	56	theme	adult	373:377	arg1	asthma					391:396	adult and elderly asthma	373:396	adult and elderly asthma	373:396	However, to date, the influence of upper airway microbiome for adult and elderly asthma has not been fully elucidated.
30242844	4	57	theme	adults	486:491	arg1	microbiome					466:475	upper airway microbiome	453:475	upper airway microbiome of young adults and elderly	453:503	Here, the metagenome of upper airway microbiome of young adults and elderly was analyzed to identify their association with adult asthma.
30242844	12	58	theme	upper	1716:1720	arg1	microbiome					1729:1738	upper airway microbiome	1716:1738	upper airway microbiome	1716:1738	CONCLUSIONS These results suggest that the composition and function of upper airway microbiome could influence asthma pathogenesis, and the microbiome could play various roles depending on the age group.
30242844	9	59	theme	pollutants	1208:1217	arg1	inflammation					1172:1183	inflammation	1172:1183	inflammation	1172:1183	Genes related to lysine degradation, N-glycan biosynthesis, caprolactam degradation, and PPAR signaling pathway, which could be related to the reduction in inflammation and degradation of air pollutants, were higher in non-asthmatics.
30242844	9	59	theme	pollutants	1208:1217	arg1	degradation					1189:1199	degradation	1189:1199	degradation	1189:1199	Genes related to lysine degradation, N-glycan biosynthesis, caprolactam degradation, and PPAR signaling pathway, which could be related to the reduction in inflammation and degradation of air pollutants, were higher in non-asthmatics.
30242844	0	60	theme	functional	44:53	arg1	genes					55:59	their functional genes	38:59	their functional genes	38:59	Different upper airway microbiome and their functional genes associated with asthma in young adults and elderly individuals.
30242844	12	61	theme	airway	1722:1727	arg1	microbiome					1729:1738	upper airway microbiome	1716:1738	upper airway microbiome	1716:1738	CONCLUSIONS These results suggest that the composition and function of upper airway microbiome could influence asthma pathogenesis, and the microbiome could play various roles depending on the age group.
30242844	10	62	theme	bacteria-were	1454:1466	arg1	colonization					1427:1438	colonization	1427:1438	colonization of pathogenic bacteria-were higher in young adult asthmatic patients	1427:1507	Genes related to pentose phosphate pathway, lipopolysaccharide biosynthesis, flagella assembly, and bacterial chemotaxis-which may all be related to increased inflammation and colonization of pathogenic bacteria-were higher in young adult asthmatic patients.
30242844	10	62	theme	bacteria-were	1454:1466	arg1	inflammation					1410:1421	increased inflammation	1400:1421	increased inflammation	1400:1421	Genes related to pentose phosphate pathway, lipopolysaccharide biosynthesis, flagella assembly, and bacterial chemotaxis-which may all be related to increased inflammation and colonization of pathogenic bacteria-were higher in young adult asthmatic patients.
30242844	12	63	theme	age	1838:1840	arg1	group					1842:1846	the age group	1834:1846	the age group	1834:1846	CONCLUSIONS These results suggest that the composition and function of upper airway microbiome could influence asthma pathogenesis, and the microbiome could play various roles depending on the age group.
30242844	4	64	theme	elderly	497:503	arg1	microbiome					466:475	upper airway microbiome	453:475	upper airway microbiome of young adults and elderly	453:503	Here, the metagenome of upper airway microbiome of young adults and elderly was analyzed to identify their association with adult asthma.
30242844	5	65	theme	non-asthmatic	660:672	arg1	subjects					674:681	non-asthmatic subjects	660:681	non-asthmatic subjects	660:681	METHODS Nasopharyngeal swabs were collected from young adult and elderly asthma patients and non-asthmatic subjects.
30242844	6	66	theme	microbiome	732:741	arg1	compositions					688:699	compositions	688:699	compositions	688:699	The compositions and functional genes of airway microbiome were analyzed by high-throughput sequencing.
30242844	6	66	theme	microbiome	732:741	arg1	genes					716:720	functional genes	705:720	functional genes	705:720	The compositions and functional genes of airway microbiome were analyzed by high-throughput sequencing.
30242844	1	67	theme	BACKGROUND	125:134	arg1	Microbes					136:143	BACKGROUND Microbes	125:143	BACKGROUND Microbes in the airway	125:157	BACKGROUND Microbes in the airway have been shown to be associated with the pathogenesis of asthma.
30242844	10	68	theme	related	1257:1263	arg1	Genes					1251:1255	Genes	1251:1255	Genes related to pentose phosphate pathway, lipopolysaccharide biosynthesis, flagella assembly, and bacterial chemotaxis-which	1251:1376	Genes related to pentose phosphate pathway, lipopolysaccharide biosynthesis, flagella assembly, and bacterial chemotaxis-which may all be related to increased inflammation and colonization of pathogenic bacteria-were higher in young adult asthmatic patients.
30242844	10	69	theme	flagella	1328:1335	arg1	assembly					1337:1344	flagella assembly	1328:1344	flagella assembly	1328:1344	Genes related to pentose phosphate pathway, lipopolysaccharide biosynthesis, flagella assembly, and bacterial chemotaxis-which may all be related to increased inflammation and colonization of pathogenic bacteria-were higher in young adult asthmatic patients.
30242844	12	70	dep	composition	1688:1698	arg1	the					1684:1686	the	1684:1686	the	1684:1686	CONCLUSIONS These results suggest that the composition and function of upper airway microbiome could influence asthma pathogenesis, and the microbiome could play various roles depending on the age group.
30242844	6	71	theme	airway	725:730	arg1	microbiome					732:741	airway microbiome	725:741	airway microbiome	725:741	The compositions and functional genes of airway microbiome were analyzed by high-throughput sequencing.
30242844	9	72	theme	related	1022:1028	arg1	Genes					1016:1020	Genes	1016:1020	Genes	1016:1020	Genes related to lysine degradation, N-glycan biosynthesis, caprolactam degradation, and PPAR signaling pathway, which could be related to the reduction in inflammation and degradation of air pollutants, were higher in non-asthmatics.
30242844	1	73	theme	asthma	217:222	arg1	pathogenesis					201:212	the pathogenesis	197:212	the pathogenesis of asthma	197:222	BACKGROUND Microbes in the airway have been shown to be associated with the pathogenesis of asthma.
30242844	9	74	theme	N-glycan	1053:1060	arg1	biosynthesis					1062:1073	N-glycan biosynthesis	1053:1073	N-glycan biosynthesis	1053:1073	Genes related to lysine degradation, N-glycan biosynthesis, caprolactam degradation, and PPAR signaling pathway, which could be related to the reduction in inflammation and degradation of air pollutants, were higher in non-asthmatics.
30242844	2	75	theme	airway	235:240	arg1	microbiome					242:251	The upper airway microbiome	225:251	The upper airway microbiome	225:251	The upper airway microbiome influences the dysbiosis of the lower airway microbiome.
30242844	8	76	theme	Different	946:954	arg1	bacteria					956:963	Different bacteria	946:963	Different bacteria	946:963	Different bacteria were related to FEV1% predicted in each age group.
30242844	5	77	dep	METHODS	567:573	arg1	collected					601:609	collected	601:609	were collected from young adult and elderly asthma patients and non-asthmatic subjects	596:681	METHODS Nasopharyngeal swabs were collected from young adult and elderly asthma patients and non-asthmatic subjects.
30242844	4	78	theme	microbiome	466:475	arg1	metagenome					439:448	the metagenome	435:448	the metagenome of upper airway microbiome of young adults and elderly	435:503	Here, the metagenome of upper airway microbiome of young adults and elderly was analyzed to identify their association with adult asthma.
30242844	2	79	theme	upper	229:233	arg1	microbiome					242:251	The upper airway microbiome	225:251	The upper airway microbiome	225:251	The upper airway microbiome influences the dysbiosis of the lower airway microbiome.
30242844	3	80	theme	microbiome	358:367	arg1	influence					332:340	the influence	328:340	the influence of upper airway microbiome for adult and elderly asthma	328:396	However, to date, the influence of upper airway microbiome for adult and elderly asthma has not been fully elucidated.
30242844	9	81	theme	lysine	1033:1038	arg1	degradation					1040:1050	lysine degradation	1033:1050	lysine degradation	1033:1050	Genes related to lysine degradation, N-glycan biosynthesis, caprolactam degradation, and PPAR signaling pathway, which could be related to the reduction in inflammation and degradation of air pollutants, were higher in non-asthmatics.
30242844	12	82	theme	asthma	1756:1761	arg1	pathogenesis					1763:1774	asthma pathogenesis	1756:1774	asthma pathogenesis	1756:1774	CONCLUSIONS These results suggest that the composition and function of upper airway microbiome could influence asthma pathogenesis, and the microbiome could play various roles depending on the age group.
30786150	3	0	theme	tunable	787:793	arg1	property					807:814	tunable rheological property	787:814	tunable rheological property	787:814	These hydrogels exhibit high swelling, degradability, tunable rheological property, and similar or superior mechanical properties to human skin.
30786150	1	1	theme	radical	325:331	arg1	ability					344:350	radical scavenging ability	325:350	radical scavenging ability	325:350	Developing injectable nanocomposite conductive hydrogel dressings with multifunctions including adhesiveness, antibacterial, and radical scavenging ability and good mechanical property to enhance full-thickness skin wound regeneration is highly desirable in clinical application.
30786150	7	2	theme	drug	1653:1656	arg1	property					1666:1673	sustained drug release property	1643:1673	sustained drug release property to promote complete skin regeneration	1643:1711	In summary, these adhesive hemostatic antioxidative conductive hydrogels with sustained drug release property to promote complete skin regeneration are an excellent wound dressing for full-thickness skin repair.
30786150	7	3	theme	adhesive	1583:1590	arg1	hydrogels					1628:1636	these adhesive hemostatic antioxidative conductive hydrogels	1577:1636	these adhesive hemostatic antioxidative conductive hydrogels with sustained drug release property to promote complete skin regeneration	1577:1711	In summary, these adhesive hemostatic antioxidative conductive hydrogels with sustained drug release property to promote complete skin regeneration are an excellent wound dressing for full-thickness skin repair.
30786150	7	3	theme	adhesive	1583:1590	arg1	dressing					1736:1743	an excellent wound dressing	1717:1743	an excellent wound dressing for full-thickness skin repair	1717:1774	In summary, these adhesive hemostatic antioxidative conductive hydrogels with sustained drug release property to promote complete skin regeneration are an excellent wound dressing for full-thickness skin repair.
30786150	6	4	theme	wound	1424:1428	arg1	closure					1430:1436	wound closure	1424:1436	wound closure	1424:1436	Furthermore, the hydrogel dressings significantly enhance vascularization by upregulating growth factor expression of CD31 and improve the granulation tissue thickness and collagen deposition, all of which promote wound closure and contribute to a better therapeutic effect than the commercial Tegaderm films group in a mouse full-thickness wounds model.
30786150	6	5	theme	CD31	1328:1331	arg1	expression					1314:1323	growth factor expression	1300:1323	growth factor expression of CD31	1300:1331	Furthermore, the hydrogel dressings significantly enhance vascularization by upregulating growth factor expression of CD31 and improve the granulation tissue thickness and collagen deposition, all of which promote wound closure and contribute to a better therapeutic effect than the commercial Tegaderm films group in a mouse full-thickness wounds model.
30786150	0	6	theme	Adhesive	0:7	arg1	Hydrogels					52:60	Adhesive Hemostatic Conducting Injectable Composite Hydrogels	0:60	Adhesive Hemostatic Conducting Injectable Composite Hydrogels with Sustained Drug Release and Photothermal Antibacterial Activity	0:128	Adhesive Hemostatic Conducting Injectable Composite Hydrogels with Sustained Drug Release and Photothermal Antibacterial Activity to Promote Full-Thickness Skin Regeneration During Wound Healing.
30786150	1	7	theme	nanocomposite	218:230	arg1	dressings					252:260	injectable nanocomposite conductive hydrogel dressings	207:260	injectable nanocomposite conductive hydrogel dressings with multifunctions including adhesiveness, antibacterial, and radical scavenging ability	207:350	Developing injectable nanocomposite conductive hydrogel dressings with multifunctions including adhesiveness, antibacterial, and radical scavenging ability and good mechanical property to enhance full-thickness skin wound regeneration is highly desirable in clinical application.
30786150	7	8	theme	conductive	1617:1626	arg1	hydrogels					1628:1636	these adhesive hemostatic antioxidative conductive hydrogels	1577:1636	these adhesive hemostatic antioxidative conductive hydrogels with sustained drug release property to promote complete skin regeneration	1577:1711	In summary, these adhesive hemostatic antioxidative conductive hydrogels with sustained drug release property to promote complete skin regeneration are an excellent wound dressing for full-thickness skin repair.
30786150	7	8	theme	conductive	1617:1626	arg1	dressing					1736:1743	an excellent wound dressing	1717:1743	an excellent wound dressing for full-thickness skin repair	1717:1774	In summary, these adhesive hemostatic antioxidative conductive hydrogels with sustained drug release property to promote complete skin regeneration are an excellent wound dressing for full-thickness skin repair.
30786150	2	9	theme	wound	717:721	arg1	dressing					723:730	wound dressing	717:730	wound dressing	717:730	Herein, a series of adhesive hemostatic antioxidant conductive photothermal antibacterial hydrogels based on hyaluronic acid-graft-dopamine and reduced graphene oxide (rGO) using a H2 O2 /HPR (horseradish peroxidase) system are prepared for wound dressing.
30786150	1	10	with	property	372:379	arg1	antibacterial					306:318	antibacterial	306:318	antibacterial	306:318	Developing injectable nanocomposite conductive hydrogel dressings with multifunctions including adhesiveness, antibacterial, and radical scavenging ability and good mechanical property to enhance full-thickness skin wound regeneration is highly desirable in clinical application.
30786150	1	10	with	property	372:379	arg1	adhesiveness					292:303	adhesiveness	292:303	adhesiveness	292:303	Developing injectable nanocomposite conductive hydrogel dressings with multifunctions including adhesiveness, antibacterial, and radical scavenging ability and good mechanical property to enhance full-thickness skin wound regeneration is highly desirable in clinical application.
30786150	1	10	with	property	372:379	arg1	multifunctions					267:280	multifunctions	267:280	multifunctions including adhesiveness, antibacterial, and radical scavenging ability	267:350	Developing injectable nanocomposite conductive hydrogel dressings with multifunctions including adhesiveness, antibacterial, and radical scavenging ability and good mechanical property to enhance full-thickness skin wound regeneration is highly desirable in clinical application.
30786150	1	10	with	property	372:379	arg1	ability					344:350	radical scavenging ability	325:350	radical scavenging ability	325:350	Developing injectable nanocomposite conductive hydrogel dressings with multifunctions including adhesiveness, antibacterial, and radical scavenging ability and good mechanical property to enhance full-thickness skin wound regeneration is highly desirable in clinical application.
30786150	4	11	theme	NIR	1009:1011	arg1	irradiation					1013:1023	NIR irradiation	1009:1023	NIR irradiation	1009:1023	The polydopamine endowed antioxidant activity, tissue adhesiveness and hemostatic ability, self-healing ability, conductivity, and NIR irradiation enhanced in vivo antibacterial behavior of the hydrogels are investigated.
30786150	1	12	theme	hydrogel	243:250	arg1	dressings					252:260	injectable nanocomposite conductive hydrogel dressings	207:260	injectable nanocomposite conductive hydrogel dressings with multifunctions including adhesiveness, antibacterial, and radical scavenging ability	207:350	Developing injectable nanocomposite conductive hydrogel dressings with multifunctions including adhesiveness, antibacterial, and radical scavenging ability and good mechanical property to enhance full-thickness skin wound regeneration is highly desirable in clinical application.
30786150	4	13	theme	antibacterial	1042:1054	arg1	behavior					1056:1063	antibacterial behavior of the hydrogels are investigated	1042:1097	antibacterial behavior of the hydrogels are investigated	1042:1097	The polydopamine endowed antioxidant activity, tissue adhesiveness and hemostatic ability, self-healing ability, conductivity, and NIR irradiation enhanced in vivo antibacterial behavior of the hydrogels are investigated.
30786150	6	14	theme	growth	1300:1305	arg1	factor					1307:1312	growth factor	1300:1312	growth factor expression of CD31	1300:1331	Furthermore, the hydrogel dressings significantly enhance vascularization by upregulating growth factor expression of CD31 and improve the granulation tissue thickness and collagen deposition, all of which promote wound closure and contribute to a better therapeutic effect than the commercial Tegaderm films group in a mouse full-thickness wounds model.
30786150	3	15	theme	mechanical	841:850	arg1	properties					852:861	similar or superior mechanical properties	821:861	similar or superior mechanical properties to human skin	821:875	These hydrogels exhibit high swelling, degradability, tunable rheological property, and similar or superior mechanical properties to human skin.
30786150	0	16	theme	Full-Thickness	141:154	arg1	Regeneration					161:172	Full-Thickness Skin Regeneration	141:172	Full-Thickness Skin Regeneration	141:172	Adhesive Hemostatic Conducting Injectable Composite Hydrogels with Sustained Drug Release and Photothermal Antibacterial Activity to Promote Full-Thickness Skin Regeneration During Wound Healing.
30786150	1	17	from	desirable	441:449	arg1	application					463:473	clinical application	454:473	clinical application	454:473	Developing injectable nanocomposite conductive hydrogel dressings with multifunctions including adhesiveness, antibacterial, and radical scavenging ability and good mechanical property to enhance full-thickness skin wound regeneration is highly desirable in clinical application.
30786150	6	18	theme	collagen	1382:1389	arg1	deposition					1391:1400	collagen deposition	1382:1400	collagen deposition	1382:1400	Furthermore, the hydrogel dressings significantly enhance vascularization by upregulating growth factor expression of CD31 and improve the granulation tissue thickness and collagen deposition, all of which promote wound closure and contribute to a better therapeutic effect than the commercial Tegaderm films group in a mouse full-thickness wounds model.
30786150	0	19	theme	Wound	181:185	arg1	Healing					187:193	Wound Healing	181:193	Wound Healing	181:193	Adhesive Hemostatic Conducting Injectable Composite Hydrogels with Sustained Drug Release and Photothermal Antibacterial Activity to Promote Full-Thickness Skin Regeneration During Wound Healing.
30786150	5	20	theme	drug	1170:1173	arg1	capacity					1183:1190	sustained drug release capacity	1160:1190	sustained drug release capacity of the hydrogels	1160:1207	Moreover, drug release and zone of inhibition tests confirm sustained drug release capacity of the hydrogels.
30786150	6	21	dep	thickness	1368:1376	arg1	the					1345:1347	the	1345:1347	the	1345:1347	Furthermore, the hydrogel dressings significantly enhance vascularization by upregulating growth factor expression of CD31 and improve the granulation tissue thickness and collagen deposition, all of which promote wound closure and contribute to a better therapeutic effect than the commercial Tegaderm films group in a mouse full-thickness wounds model.
30786150	2	22	theme	graphene	628:635	arg1	rGO					644:646	rGO	644:646	rGO	644:646	Herein, a series of adhesive hemostatic antioxidant conductive photothermal antibacterial hydrogels based on hyaluronic acid-graft-dopamine and reduced graphene oxide (rGO) using a H2 O2 /HPR (horseradish peroxidase) system are prepared for wound dressing.
30786150	2	22	theme	graphene	628:635	arg1	oxide					637:641	reduced graphene oxide	620:641	reduced graphene oxide (rGO)	620:647	Herein, a series of adhesive hemostatic antioxidant conductive photothermal antibacterial hydrogels based on hyaluronic acid-graft-dopamine and reduced graphene oxide (rGO) using a H2 O2 /HPR (horseradish peroxidase) system are prepared for wound dressing.
30786150	6	23	theme	granulation	1349:1359	arg1	thickness					1368:1376	granulation tissue thickness	1349:1376	granulation tissue thickness	1349:1376	Furthermore, the hydrogel dressings significantly enhance vascularization by upregulating growth factor expression of CD31 and improve the granulation tissue thickness and collagen deposition, all of which promote wound closure and contribute to a better therapeutic effect than the commercial Tegaderm films group in a mouse full-thickness wounds model.
30786150	6	24	theme	full-thickness	1536:1549	arg1	model					1558:1562	a mouse full-thickness wounds model	1528:1562	a mouse full-thickness wounds model	1528:1562	Furthermore, the hydrogel dressings significantly enhance vascularization by upregulating growth factor expression of CD31 and improve the granulation tissue thickness and collagen deposition, all of which promote wound closure and contribute to a better therapeutic effect than the commercial Tegaderm films group in a mouse full-thickness wounds model.
30786150	1	25	theme	wound	412:416	arg1	regeneration					418:429	full-thickness skin wound regeneration	392:429	full-thickness skin wound regeneration	392:429	Developing injectable nanocomposite conductive hydrogel dressings with multifunctions including adhesiveness, antibacterial, and radical scavenging ability and good mechanical property to enhance full-thickness skin wound regeneration is highly desirable in clinical application.
30786150	7	26	theme	full-thickness	1749:1762	arg1	repair					1769:1774	full-thickness skin repair	1749:1774	full-thickness skin repair	1749:1774	In summary, these adhesive hemostatic antioxidative conductive hydrogels with sustained drug release property to promote complete skin regeneration are an excellent wound dressing for full-thickness skin repair.
30786150	0	27	theme	Antibacterial	107:119	arg1	Activity					121:128	Photothermal Antibacterial Activity	94:128	Photothermal Antibacterial Activity	94:128	Adhesive Hemostatic Conducting Injectable Composite Hydrogels with Sustained Drug Release and Photothermal Antibacterial Activity to Promote Full-Thickness Skin Regeneration During Wound Healing.
30786150	6	28	theme	Tegaderm	1504:1511	arg1	group					1519:1523	the commercial Tegaderm films group	1489:1523	the commercial Tegaderm films group in a mouse full-thickness wounds model	1489:1562	Furthermore, the hydrogel dressings significantly enhance vascularization by upregulating growth factor expression of CD31 and improve the granulation tissue thickness and collagen deposition, all of which promote wound closure and contribute to a better therapeutic effect than the commercial Tegaderm films group in a mouse full-thickness wounds model.
30786150	1	29	theme	clinical	454:461	arg1	application					463:473	clinical application	454:473	clinical application	454:473	Developing injectable nanocomposite conductive hydrogel dressings with multifunctions including adhesiveness, antibacterial, and radical scavenging ability and good mechanical property to enhance full-thickness skin wound regeneration is highly desirable in clinical application.
30786150	5	30	theme	drug	1110:1113	arg1	release					1115:1121	drug release	1110:1121	drug release	1110:1121	Moreover, drug release and zone of inhibition tests confirm sustained drug release capacity of the hydrogels.
30786150	2	31	theme	antibacterial	552:564	arg1	hydrogels					566:574	adhesive hemostatic antioxidant conductive photothermal antibacterial hydrogels	496:574	adhesive hemostatic antioxidant conductive photothermal antibacterial hydrogels based on hyaluronic acid-graft-dopamine and reduced graphene oxide (rGO) using a H2 O2 /HPR (horseradish peroxidase) system	496:698	Herein, a series of adhesive hemostatic antioxidant conductive photothermal antibacterial hydrogels based on hyaluronic acid-graft-dopamine and reduced graphene oxide (rGO) using a H2 O2 /HPR (horseradish peroxidase) system are prepared for wound dressing.
30786150	1	32	from	application	463:473	arg1	desirable					441:449	desirable	441:449	desirable	441:449	Developing injectable nanocomposite conductive hydrogel dressings with multifunctions including adhesiveness, antibacterial, and radical scavenging ability and good mechanical property to enhance full-thickness skin wound regeneration is highly desirable in clinical application.
30786150	2	33	theme	conductive	528:537	arg1	hydrogels					566:574	adhesive hemostatic antioxidant conductive photothermal antibacterial hydrogels	496:574	adhesive hemostatic antioxidant conductive photothermal antibacterial hydrogels based on hyaluronic acid-graft-dopamine and reduced graphene oxide (rGO) using a H2 O2 /HPR (horseradish peroxidase) system	496:698	Herein, a series of adhesive hemostatic antioxidant conductive photothermal antibacterial hydrogels based on hyaluronic acid-graft-dopamine and reduced graphene oxide (rGO) using a H2 O2 /HPR (horseradish peroxidase) system are prepared for wound dressing.
30786150	2	34	theme	hemostatic	505:514	arg1	hydrogels					566:574	adhesive hemostatic antioxidant conductive photothermal antibacterial hydrogels	496:574	adhesive hemostatic antioxidant conductive photothermal antibacterial hydrogels based on hyaluronic acid-graft-dopamine and reduced graphene oxide (rGO) using a H2 O2 /HPR (horseradish peroxidase) system	496:698	Herein, a series of adhesive hemostatic antioxidant conductive photothermal antibacterial hydrogels based on hyaluronic acid-graft-dopamine and reduced graphene oxide (rGO) using a H2 O2 /HPR (horseradish peroxidase) system are prepared for wound dressing.
30786150	0	35	theme	Conducting	20:29	arg1	Hydrogels					52:60	Adhesive Hemostatic Conducting Injectable Composite Hydrogels	0:60	Adhesive Hemostatic Conducting Injectable Composite Hydrogels with Sustained Drug Release and Photothermal Antibacterial Activity	0:128	Adhesive Hemostatic Conducting Injectable Composite Hydrogels with Sustained Drug Release and Photothermal Antibacterial Activity to Promote Full-Thickness Skin Regeneration During Wound Healing.
30786150	1	36	theme	mechanical	361:370	arg1	property					372:379	good mechanical property	356:379	good mechanical property	356:379	Developing injectable nanocomposite conductive hydrogel dressings with multifunctions including adhesiveness, antibacterial, and radical scavenging ability and good mechanical property to enhance full-thickness skin wound regeneration is highly desirable in clinical application.
30786150	7	37	from	dressing	1736:1743	arg1	summary					1568:1574	summary	1568:1574	summary	1568:1574	In summary, these adhesive hemostatic antioxidative conductive hydrogels with sustained drug release property to promote complete skin regeneration are an excellent wound dressing for full-thickness skin repair.
30786150	2	38	theme	hyaluronic	585:594	arg1	acid-graft-dopamine					596:614	hyaluronic acid-graft-dopamine	585:614	hyaluronic acid-graft-dopamine	585:614	Herein, a series of adhesive hemostatic antioxidant conductive photothermal antibacterial hydrogels based on hyaluronic acid-graft-dopamine and reduced graphene oxide (rGO) using a H2 O2 /HPR (horseradish peroxidase) system are prepared for wound dressing.
30786150	3	39	theme	high	757:760	arg1	swelling					762:769	high swelling	757:769	high swelling	757:769	These hydrogels exhibit high swelling, degradability, tunable rheological property, and similar or superior mechanical properties to human skin.
30786150	0	40	theme	Composite	42:50	arg1	Hydrogels					52:60	Adhesive Hemostatic Conducting Injectable Composite Hydrogels	0:60	Adhesive Hemostatic Conducting Injectable Composite Hydrogels with Sustained Drug Release and Photothermal Antibacterial Activity	0:128	Adhesive Hemostatic Conducting Injectable Composite Hydrogels with Sustained Drug Release and Photothermal Antibacterial Activity to Promote Full-Thickness Skin Regeneration During Wound Healing.
30786150	5	41	theme	tests	1146:1150	arg1	zone					1127:1130	zone	1127:1130	zone of inhibition tests	1127:1150	Moreover, drug release and zone of inhibition tests confirm sustained drug release capacity of the hydrogels.
30786150	5	41	theme	tests	1146:1150	arg1	release					1115:1121	drug release	1110:1121	drug release	1110:1121	Moreover, drug release and zone of inhibition tests confirm sustained drug release capacity of the hydrogels.
30786150	0	42	theme	Drug	77:80	arg1	Release					82:88	Sustained Drug Release	67:88	Sustained Drug Release	67:88	Adhesive Hemostatic Conducting Injectable Composite Hydrogels with Sustained Drug Release and Photothermal Antibacterial Activity to Promote Full-Thickness Skin Regeneration During Wound Healing.
30786150	6	43	dep	dressings	1236:1244	arg1	enhance					1260:1266	enhance	1260:1266	enhance vascularization by upregulating growth factor expression of CD31	1260:1331	Furthermore, the hydrogel dressings significantly enhance vascularization by upregulating growth factor expression of CD31 and improve the granulation tissue thickness and collagen deposition, all of which promote wound closure and contribute to a better therapeutic effect than the commercial Tegaderm films group in a mouse full-thickness wounds model.
30786150	6	43	dep	dressings	1236:1244	arg1	improve					1337:1343	improve	1337:1343	improve the granulation tissue thickness and collagen deposition, all of which promote wound closure and contribute to a better therapeutic effect than the commercial Tegaderm films group in a mouse full-thickness wounds model	1337:1562	Furthermore, the hydrogel dressings significantly enhance vascularization by upregulating growth factor expression of CD31 and improve the granulation tissue thickness and collagen deposition, all of which promote wound closure and contribute to a better therapeutic effect than the commercial Tegaderm films group in a mouse full-thickness wounds model.
30786150	1	44	theme	full-thickness	392:405	arg1	regeneration					418:429	full-thickness skin wound regeneration	392:429	full-thickness skin wound regeneration	392:429	Developing injectable nanocomposite conductive hydrogel dressings with multifunctions including adhesiveness, antibacterial, and radical scavenging ability and good mechanical property to enhance full-thickness skin wound regeneration is highly desirable in clinical application.
30786150	7	45	theme	complete	1686:1693	arg1	regeneration					1700:1711	complete skin regeneration	1686:1711	complete skin regeneration	1686:1711	In summary, these adhesive hemostatic antioxidative conductive hydrogels with sustained drug release property to promote complete skin regeneration are an excellent wound dressing for full-thickness skin repair.
30786150	5	46	theme	sustained	1160:1168	arg1	capacity					1183:1190	sustained drug release capacity	1160:1190	sustained drug release capacity of the hydrogels	1160:1207	Moreover, drug release and zone of inhibition tests confirm sustained drug release capacity of the hydrogels.
30786150	4	47	theme	antioxidant	903:913	arg1	activity					915:922	The polydopamine endowed antioxidant activity	878:922	The polydopamine endowed antioxidant activity	878:922	The polydopamine endowed antioxidant activity, tissue adhesiveness and hemostatic ability, self-healing ability, conductivity, and NIR irradiation enhanced in vivo antibacterial behavior of the hydrogels are investigated.
30786150	7	48	theme	release	1658:1664	arg1	property					1666:1673	sustained drug release property	1643:1673	sustained drug release property to promote complete skin regeneration	1643:1711	In summary, these adhesive hemostatic antioxidative conductive hydrogels with sustained drug release property to promote complete skin regeneration are an excellent wound dressing for full-thickness skin repair.
30786150	6	49	theme	mouse	1530:1534	arg1	model					1558:1562	a mouse full-thickness wounds model	1528:1562	a mouse full-thickness wounds model	1528:1562	Furthermore, the hydrogel dressings significantly enhance vascularization by upregulating growth factor expression of CD31 and improve the granulation tissue thickness and collagen deposition, all of which promote wound closure and contribute to a better therapeutic effect than the commercial Tegaderm films group in a mouse full-thickness wounds model.
30786150	7	50	theme	sustained	1643:1651	arg1	property					1666:1673	sustained drug release property	1643:1673	sustained drug release property to promote complete skin regeneration	1643:1711	In summary, these adhesive hemostatic antioxidative conductive hydrogels with sustained drug release property to promote complete skin regeneration are an excellent wound dressing for full-thickness skin repair.
30786150	3	51	theme	rheological	795:805	arg1	property					807:814	tunable rheological property	787:814	tunable rheological property	787:814	These hydrogels exhibit high swelling, degradability, tunable rheological property, and similar or superior mechanical properties to human skin.
30786150	4	52	theme	hemostatic	949:958	arg1	ability					960:966	hemostatic ability	949:966	hemostatic ability	949:966	The polydopamine endowed antioxidant activity, tissue adhesiveness and hemostatic ability, self-healing ability, conductivity, and NIR irradiation enhanced in vivo antibacterial behavior of the hydrogels are investigated.
30786150	1	53	theme	scavenging	333:342	arg1	ability					344:350	radical scavenging ability	325:350	radical scavenging ability	325:350	Developing injectable nanocomposite conductive hydrogel dressings with multifunctions including adhesiveness, antibacterial, and radical scavenging ability and good mechanical property to enhance full-thickness skin wound regeneration is highly desirable in clinical application.
30786150	7	54	theme	hemostatic	1592:1601	arg1	hydrogels					1628:1636	these adhesive hemostatic antioxidative conductive hydrogels	1577:1636	these adhesive hemostatic antioxidative conductive hydrogels with sustained drug release property to promote complete skin regeneration	1577:1711	In summary, these adhesive hemostatic antioxidative conductive hydrogels with sustained drug release property to promote complete skin regeneration are an excellent wound dressing for full-thickness skin repair.
30786150	7	54	theme	hemostatic	1592:1601	arg1	dressing					1736:1743	an excellent wound dressing	1717:1743	an excellent wound dressing for full-thickness skin repair	1717:1774	In summary, these adhesive hemostatic antioxidative conductive hydrogels with sustained drug release property to promote complete skin regeneration are an excellent wound dressing for full-thickness skin repair.
30786150	3	55	theme	superior	832:839	arg1	properties					852:861	similar or superior mechanical properties	821:861	similar or superior mechanical properties to human skin	821:875	These hydrogels exhibit high swelling, degradability, tunable rheological property, and similar or superior mechanical properties to human skin.
30786150	1	56	theme	injectable	207:216	arg1	dressings					252:260	injectable nanocomposite conductive hydrogel dressings	207:260	injectable nanocomposite conductive hydrogel dressings with multifunctions including adhesiveness, antibacterial, and radical scavenging ability	207:350	Developing injectable nanocomposite conductive hydrogel dressings with multifunctions including adhesiveness, antibacterial, and radical scavenging ability and good mechanical property to enhance full-thickness skin wound regeneration is highly desirable in clinical application.
30786150	7	57	theme	antioxidative	1603:1615	arg1	hydrogels					1628:1636	these adhesive hemostatic antioxidative conductive hydrogels	1577:1636	these adhesive hemostatic antioxidative conductive hydrogels with sustained drug release property to promote complete skin regeneration	1577:1711	In summary, these adhesive hemostatic antioxidative conductive hydrogels with sustained drug release property to promote complete skin regeneration are an excellent wound dressing for full-thickness skin repair.
30786150	7	57	theme	antioxidative	1603:1615	arg1	dressing					1736:1743	an excellent wound dressing	1717:1743	an excellent wound dressing for full-thickness skin repair	1717:1774	In summary, these adhesive hemostatic antioxidative conductive hydrogels with sustained drug release property to promote complete skin regeneration are an excellent wound dressing for full-thickness skin repair.
30786150	3	58	theme	similar	821:827	arg1	properties					852:861	similar or superior mechanical properties	821:861	similar or superior mechanical properties to human skin	821:875	These hydrogels exhibit high swelling, degradability, tunable rheological property, and similar or superior mechanical properties to human skin.
30786150	1	59	theme	conductive	232:241	arg1	dressings					252:260	injectable nanocomposite conductive hydrogel dressings	207:260	injectable nanocomposite conductive hydrogel dressings with multifunctions including adhesiveness, antibacterial, and radical scavenging ability	207:350	Developing injectable nanocomposite conductive hydrogel dressings with multifunctions including adhesiveness, antibacterial, and radical scavenging ability and good mechanical property to enhance full-thickness skin wound regeneration is highly desirable in clinical application.
30786150	4	60	theme	self-healing	969:980	arg1	ability					982:988	self-healing ability	969:988	self-healing ability	969:988	The polydopamine endowed antioxidant activity, tissue adhesiveness and hemostatic ability, self-healing ability, conductivity, and NIR irradiation enhanced in vivo antibacterial behavior of the hydrogels are investigated.
30786150	6	61	theme	factor	1307:1312	arg1	expression					1314:1323	growth factor expression	1300:1323	growth factor expression of CD31	1300:1331	Furthermore, the hydrogel dressings significantly enhance vascularization by upregulating growth factor expression of CD31 and improve the granulation tissue thickness and collagen deposition, all of which promote wound closure and contribute to a better therapeutic effect than the commercial Tegaderm films group in a mouse full-thickness wounds model.
30786150	3	62	theme	human	866:870	arg1	skin					872:875	human skin	866:875	human skin	866:875	These hydrogels exhibit high swelling, degradability, tunable rheological property, and similar or superior mechanical properties to human skin.
30786150	6	63	theme	commercial	1493:1502	arg1	group					1519:1523	the commercial Tegaderm films group	1489:1523	the commercial Tegaderm films group in a mouse full-thickness wounds model	1489:1562	Furthermore, the hydrogel dressings significantly enhance vascularization by upregulating growth factor expression of CD31 and improve the granulation tissue thickness and collagen deposition, all of which promote wound closure and contribute to a better therapeutic effect than the commercial Tegaderm films group in a mouse full-thickness wounds model.
30786150	2	64	theme	H2	657:658	arg1	/HPR					663:666	H2 O2 /HPR	657:666	a H2 O2 /HPR (horseradish peroxidase) system	655:698	Herein, a series of adhesive hemostatic antioxidant conductive photothermal antibacterial hydrogels based on hyaluronic acid-graft-dopamine and reduced graphene oxide (rGO) using a H2 O2 /HPR (horseradish peroxidase) system are prepared for wound dressing.
30786150	2	64	theme	H2	657:658	arg1	peroxidase					681:690	horseradish peroxidase	669:690	horseradish peroxidase	669:690	Herein, a series of adhesive hemostatic antioxidant conductive photothermal antibacterial hydrogels based on hyaluronic acid-graft-dopamine and reduced graphene oxide (rGO) using a H2 O2 /HPR (horseradish peroxidase) system are prepared for wound dressing.
30786150	5	65	theme	hydrogels	1199:1207	arg1	capacity					1183:1190	sustained drug release capacity	1160:1190	sustained drug release capacity of the hydrogels	1160:1207	Moreover, drug release and zone of inhibition tests confirm sustained drug release capacity of the hydrogels.
30786150	6	66	theme	therapeutic	1465:1475	arg1	effect					1477:1482	a better therapeutic effect	1456:1482	a better therapeutic effect than the commercial Tegaderm films group in a mouse full-thickness wounds model	1456:1562	Furthermore, the hydrogel dressings significantly enhance vascularization by upregulating growth factor expression of CD31 and improve the granulation tissue thickness and collagen deposition, all of which promote wound closure and contribute to a better therapeutic effect than the commercial Tegaderm films group in a mouse full-thickness wounds model.
30786150	0	67	theme	Skin	156:159	arg1	Regeneration					161:172	Full-Thickness Skin Regeneration	141:172	Full-Thickness Skin Regeneration	141:172	Adhesive Hemostatic Conducting Injectable Composite Hydrogels with Sustained Drug Release and Photothermal Antibacterial Activity to Promote Full-Thickness Skin Regeneration During Wound Healing.
30786150	2	68	theme	horseradish	669:679	arg1	/HPR					663:666	H2 O2 /HPR	657:666	a H2 O2 /HPR (horseradish peroxidase) system	655:698	Herein, a series of adhesive hemostatic antioxidant conductive photothermal antibacterial hydrogels based on hyaluronic acid-graft-dopamine and reduced graphene oxide (rGO) using a H2 O2 /HPR (horseradish peroxidase) system are prepared for wound dressing.
30786150	2	68	theme	horseradish	669:679	arg1	peroxidase					681:690	horseradish peroxidase	669:690	horseradish peroxidase	669:690	Herein, a series of adhesive hemostatic antioxidant conductive photothermal antibacterial hydrogels based on hyaluronic acid-graft-dopamine and reduced graphene oxide (rGO) using a H2 O2 /HPR (horseradish peroxidase) system are prepared for wound dressing.
30786150	5	69	theme	release	1175:1181	arg1	capacity					1183:1190	sustained drug release capacity	1160:1190	sustained drug release capacity of the hydrogels	1160:1207	Moreover, drug release and zone of inhibition tests confirm sustained drug release capacity of the hydrogels.
30786150	2	70	theme	/HPR	663:666	arg1	system					693:698	a H2 O2 /HPR (horseradish peroxidase) system	655:698	a H2 O2 /HPR (horseradish peroxidase) system	655:698	Herein, a series of adhesive hemostatic antioxidant conductive photothermal antibacterial hydrogels based on hyaluronic acid-graft-dopamine and reduced graphene oxide (rGO) using a H2 O2 /HPR (horseradish peroxidase) system are prepared for wound dressing.
30786150	6	71	theme	tissue	1361:1366	arg1	thickness					1368:1376	granulation tissue thickness	1349:1376	granulation tissue thickness	1349:1376	Furthermore, the hydrogel dressings significantly enhance vascularization by upregulating growth factor expression of CD31 and improve the granulation tissue thickness and collagen deposition, all of which promote wound closure and contribute to a better therapeutic effect than the commercial Tegaderm films group in a mouse full-thickness wounds model.
30786150	6	72	theme	wounds	1551:1556	arg1	model					1558:1562	a mouse full-thickness wounds model	1528:1562	a mouse full-thickness wounds model	1528:1562	Furthermore, the hydrogel dressings significantly enhance vascularization by upregulating growth factor expression of CD31 and improve the granulation tissue thickness and collagen deposition, all of which promote wound closure and contribute to a better therapeutic effect than the commercial Tegaderm films group in a mouse full-thickness wounds model.
30786150	7	73	theme	skin	1764:1767	arg1	repair					1769:1774	full-thickness skin repair	1749:1774	full-thickness skin repair	1749:1774	In summary, these adhesive hemostatic antioxidative conductive hydrogels with sustained drug release property to promote complete skin regeneration are an excellent wound dressing for full-thickness skin repair.
30786150	2	74	theme	reduced	620:626	arg1	rGO					644:646	rGO	644:646	rGO	644:646	Herein, a series of adhesive hemostatic antioxidant conductive photothermal antibacterial hydrogels based on hyaluronic acid-graft-dopamine and reduced graphene oxide (rGO) using a H2 O2 /HPR (horseradish peroxidase) system are prepared for wound dressing.
30786150	2	74	theme	reduced	620:626	arg1	oxide					637:641	reduced graphene oxide	620:641	reduced graphene oxide (rGO)	620:647	Herein, a series of adhesive hemostatic antioxidant conductive photothermal antibacterial hydrogels based on hyaluronic acid-graft-dopamine and reduced graphene oxide (rGO) using a H2 O2 /HPR (horseradish peroxidase) system are prepared for wound dressing.
30786150	0	75	theme	Photothermal	94:105	arg1	Activity					121:128	Photothermal Antibacterial Activity	94:128	Photothermal Antibacterial Activity	94:128	Adhesive Hemostatic Conducting Injectable Composite Hydrogels with Sustained Drug Release and Photothermal Antibacterial Activity to Promote Full-Thickness Skin Regeneration During Wound Healing.
30786150	7	76	theme	wound	1730:1734	arg1	hydrogels					1628:1636	these adhesive hemostatic antioxidative conductive hydrogels	1577:1636	these adhesive hemostatic antioxidative conductive hydrogels with sustained drug release property to promote complete skin regeneration	1577:1711	In summary, these adhesive hemostatic antioxidative conductive hydrogels with sustained drug release property to promote complete skin regeneration are an excellent wound dressing for full-thickness skin repair.
30786150	7	76	theme	wound	1730:1734	arg1	dressing					1736:1743	an excellent wound dressing	1717:1743	an excellent wound dressing for full-thickness skin repair	1717:1774	In summary, these adhesive hemostatic antioxidative conductive hydrogels with sustained drug release property to promote complete skin regeneration are an excellent wound dressing for full-thickness skin repair.
30786150	6	77	theme	films	1513:1517	arg1	group					1519:1523	the commercial Tegaderm films group	1489:1523	the commercial Tegaderm films group in a mouse full-thickness wounds model	1489:1562	Furthermore, the hydrogel dressings significantly enhance vascularization by upregulating growth factor expression of CD31 and improve the granulation tissue thickness and collagen deposition, all of which promote wound closure and contribute to a better therapeutic effect than the commercial Tegaderm films group in a mouse full-thickness wounds model.
30786150	0	78	theme	Hemostatic	9:18	arg1	Hydrogels					52:60	Adhesive Hemostatic Conducting Injectable Composite Hydrogels	0:60	Adhesive Hemostatic Conducting Injectable Composite Hydrogels with Sustained Drug Release and Photothermal Antibacterial Activity	0:128	Adhesive Hemostatic Conducting Injectable Composite Hydrogels with Sustained Drug Release and Photothermal Antibacterial Activity to Promote Full-Thickness Skin Regeneration During Wound Healing.
30786150	6	79	from	group	1519:1523	arg1	model					1558:1562	a mouse full-thickness wounds model	1528:1562	a mouse full-thickness wounds model	1528:1562	Furthermore, the hydrogel dressings significantly enhance vascularization by upregulating growth factor expression of CD31 and improve the granulation tissue thickness and collagen deposition, all of which promote wound closure and contribute to a better therapeutic effect than the commercial Tegaderm films group in a mouse full-thickness wounds model.
30786150	2	80	theme	photothermal	539:550	arg1	hydrogels					566:574	adhesive hemostatic antioxidant conductive photothermal antibacterial hydrogels	496:574	adhesive hemostatic antioxidant conductive photothermal antibacterial hydrogels based on hyaluronic acid-graft-dopamine and reduced graphene oxide (rGO) using a H2 O2 /HPR (horseradish peroxidase) system	496:698	Herein, a series of adhesive hemostatic antioxidant conductive photothermal antibacterial hydrogels based on hyaluronic acid-graft-dopamine and reduced graphene oxide (rGO) using a H2 O2 /HPR (horseradish peroxidase) system are prepared for wound dressing.
30786150	0	81	theme	Injectable	31:40	arg1	Hydrogels					52:60	Adhesive Hemostatic Conducting Injectable Composite Hydrogels	0:60	Adhesive Hemostatic Conducting Injectable Composite Hydrogels with Sustained Drug Release and Photothermal Antibacterial Activity	0:128	Adhesive Hemostatic Conducting Injectable Composite Hydrogels with Sustained Drug Release and Photothermal Antibacterial Activity to Promote Full-Thickness Skin Regeneration During Wound Healing.
30786150	1	82	theme	good	356:359	arg1	property					372:379	good mechanical property	356:379	good mechanical property	356:379	Developing injectable nanocomposite conductive hydrogel dressings with multifunctions including adhesiveness, antibacterial, and radical scavenging ability and good mechanical property to enhance full-thickness skin wound regeneration is highly desirable in clinical application.
30786150	2	83	theme	antioxidant	516:526	arg1	hydrogels					566:574	adhesive hemostatic antioxidant conductive photothermal antibacterial hydrogels	496:574	adhesive hemostatic antioxidant conductive photothermal antibacterial hydrogels based on hyaluronic acid-graft-dopamine and reduced graphene oxide (rGO) using a H2 O2 /HPR (horseradish peroxidase) system	496:698	Herein, a series of adhesive hemostatic antioxidant conductive photothermal antibacterial hydrogels based on hyaluronic acid-graft-dopamine and reduced graphene oxide (rGO) using a H2 O2 /HPR (horseradish peroxidase) system are prepared for wound dressing.
30786150	2	84	theme	adhesive	496:503	arg1	hydrogels					566:574	adhesive hemostatic antioxidant conductive photothermal antibacterial hydrogels	496:574	adhesive hemostatic antioxidant conductive photothermal antibacterial hydrogels based on hyaluronic acid-graft-dopamine and reduced graphene oxide (rGO) using a H2 O2 /HPR (horseradish peroxidase) system	496:698	Herein, a series of adhesive hemostatic antioxidant conductive photothermal antibacterial hydrogels based on hyaluronic acid-graft-dopamine and reduced graphene oxide (rGO) using a H2 O2 /HPR (horseradish peroxidase) system are prepared for wound dressing.
30786150	7	85	dep	property	1666:1673	arg1	promote					1678:1684	promote	1678:1684	to promote complete skin regeneration	1675:1711	In summary, these adhesive hemostatic antioxidative conductive hydrogels with sustained drug release property to promote complete skin regeneration are an excellent wound dressing for full-thickness skin repair.
30786150	1	86	with	dressings	252:260	arg1	antibacterial					306:318	antibacterial	306:318	antibacterial	306:318	Developing injectable nanocomposite conductive hydrogel dressings with multifunctions including adhesiveness, antibacterial, and radical scavenging ability and good mechanical property to enhance full-thickness skin wound regeneration is highly desirable in clinical application.
30786150	1	86	with	dressings	252:260	arg1	adhesiveness					292:303	adhesiveness	292:303	adhesiveness	292:303	Developing injectable nanocomposite conductive hydrogel dressings with multifunctions including adhesiveness, antibacterial, and radical scavenging ability and good mechanical property to enhance full-thickness skin wound regeneration is highly desirable in clinical application.
30786150	1	86	with	dressings	252:260	arg1	multifunctions					267:280	multifunctions	267:280	multifunctions including adhesiveness, antibacterial, and radical scavenging ability	267:350	Developing injectable nanocomposite conductive hydrogel dressings with multifunctions including adhesiveness, antibacterial, and radical scavenging ability and good mechanical property to enhance full-thickness skin wound regeneration is highly desirable in clinical application.
30786150	1	86	with	dressings	252:260	arg1	ability					344:350	radical scavenging ability	325:350	radical scavenging ability	325:350	Developing injectable nanocomposite conductive hydrogel dressings with multifunctions including adhesiveness, antibacterial, and radical scavenging ability and good mechanical property to enhance full-thickness skin wound regeneration is highly desirable in clinical application.
30786150	7	87	theme	excellent	1720:1728	arg1	hydrogels					1628:1636	these adhesive hemostatic antioxidative conductive hydrogels	1577:1636	these adhesive hemostatic antioxidative conductive hydrogels with sustained drug release property to promote complete skin regeneration	1577:1711	In summary, these adhesive hemostatic antioxidative conductive hydrogels with sustained drug release property to promote complete skin regeneration are an excellent wound dressing for full-thickness skin repair.
30786150	7	87	theme	excellent	1720:1728	arg1	dressing					1736:1743	an excellent wound dressing	1717:1743	an excellent wound dressing for full-thickness skin repair	1717:1774	In summary, these adhesive hemostatic antioxidative conductive hydrogels with sustained drug release property to promote complete skin regeneration are an excellent wound dressing for full-thickness skin repair.
30786150	5	88	theme	inhibition	1135:1144	arg1	tests					1146:1150	inhibition tests	1135:1150	inhibition tests	1135:1150	Moreover, drug release and zone of inhibition tests confirm sustained drug release capacity of the hydrogels.
30786150	4	89	theme	tissue	925:930	arg1	adhesiveness					932:943	tissue adhesiveness	925:943	tissue adhesiveness	925:943	The polydopamine endowed antioxidant activity, tissue adhesiveness and hemostatic ability, self-healing ability, conductivity, and NIR irradiation enhanced in vivo antibacterial behavior of the hydrogels are investigated.
30786150	0	90	theme	Sustained	67:75	arg1	Release					82:88	Sustained Drug Release	67:88	Sustained Drug Release	67:88	Adhesive Hemostatic Conducting Injectable Composite Hydrogels with Sustained Drug Release and Photothermal Antibacterial Activity to Promote Full-Thickness Skin Regeneration During Wound Healing.
30786150	2	91	theme	hydrogels	566:574	arg1	series					486:491	a series	484:491	a series of adhesive hemostatic antioxidant conductive photothermal antibacterial hydrogels based on hyaluronic acid-graft-dopamine and reduced graphene oxide (rGO) using a H2 O2 /HPR (horseradish peroxidase) system	484:698	Herein, a series of adhesive hemostatic antioxidant conductive photothermal antibacterial hydrogels based on hyaluronic acid-graft-dopamine and reduced graphene oxide (rGO) using a H2 O2 /HPR (horseradish peroxidase) system are prepared for wound dressing.
30786150	1	92	theme	skin	407:410	arg1	regeneration					418:429	full-thickness skin wound regeneration	392:429	full-thickness skin wound regeneration	392:429	Developing injectable nanocomposite conductive hydrogel dressings with multifunctions including adhesiveness, antibacterial, and radical scavenging ability and good mechanical property to enhance full-thickness skin wound regeneration is highly desirable in clinical application.
30786150	7	93	theme	skin	1695:1698	arg1	regeneration					1700:1711	complete skin regeneration	1686:1711	complete skin regeneration	1686:1711	In summary, these adhesive hemostatic antioxidative conductive hydrogels with sustained drug release property to promote complete skin regeneration are an excellent wound dressing for full-thickness skin repair.
30786150	4	94	theme	endowed	895:901	arg1	activity					915:922	The polydopamine endowed antioxidant activity	878:922	The polydopamine endowed antioxidant activity	878:922	The polydopamine endowed antioxidant activity, tissue adhesiveness and hemostatic ability, self-healing ability, conductivity, and NIR irradiation enhanced in vivo antibacterial behavior of the hydrogels are investigated.
30786150	7	95	with	hydrogels	1628:1636	arg1	property					1666:1673	sustained drug release property	1643:1673	sustained drug release property to promote complete skin regeneration	1643:1711	In summary, these adhesive hemostatic antioxidative conductive hydrogels with sustained drug release property to promote complete skin regeneration are an excellent wound dressing for full-thickness skin repair.
30786150	0	96	with	Hydrogels	52:60	arg1	Activity					121:128	Photothermal Antibacterial Activity	94:128	Photothermal Antibacterial Activity	94:128	Adhesive Hemostatic Conducting Injectable Composite Hydrogels with Sustained Drug Release and Photothermal Antibacterial Activity to Promote Full-Thickness Skin Regeneration During Wound Healing.
30786150	0	96	with	Hydrogels	52:60	arg1	Release					82:88	Sustained Drug Release	67:88	Sustained Drug Release	67:88	Adhesive Hemostatic Conducting Injectable Composite Hydrogels with Sustained Drug Release and Photothermal Antibacterial Activity to Promote Full-Thickness Skin Regeneration During Wound Healing.
31337466	6	0	theme	BM	1121:1122	arg1	composition					1132:1142	BM protein composition	1121:1142	BM protein composition	1121:1142	As a result, at GA ≤ 28 weeks, we observed that protein content is higher in BM for multiples (1.53 vs. 1.29 g per 100 ml), lactose concentration is greater in BM for singletons (6.72 vs. 6.34 g per 100 ml) and GA results the most relevant factor influencing BM protein composition.
31337466	3	1	theme	such	431:434	arg1	neonates					436:443	such neonates	431:443	such neonates	431:443	Multiple births represent about 3% of the whole pregnancies; such neonates result more vulnerable than full-term newborns, due to lower GA and birth weight (BW) and the higher incidence of perinatal complications.
31337466	1	2	theme	postnatal	140:148	arg1	age					150:152	postnatal age	140:152	postnatal age	140:152	Exclusive breastfeeding is currently recommended until at least 6 months of postnatal age, due to maternal breast milk (BM) unique composition and beneficial properties.
31337466	1	3	theme	beneficial	211:220	arg1	properties					222:231	beneficial properties	211:231	beneficial properties	211:231	Exclusive breastfeeding is currently recommended until at least 6 months of postnatal age, due to maternal breast milk (BM) unique composition and beneficial properties.
31337466	3	4	theme	full-term	473:481	arg1	newborns					483:490	full-term newborns	473:490	full-term newborns	473:490	Multiple births represent about 3% of the whole pregnancies; such neonates result more vulnerable than full-term newborns, due to lower GA and birth weight (BW) and the higher incidence of perinatal complications.
31337466	8	5	theme	trophic	1364:1370	arg1	role					1388:1391	a trophic and immunologic role	1362:1391	a trophic and immunologic role	1362:1391	BM from multiples shows a trophic and immunologic role, since these neonates often show lower GA and BW instead of singletons.
31337466	6	6	theme	relevant	1093:1100	arg1	factor					1102:1107	the most relevant factor	1084:1107	the most relevant factor influencing BM protein composition	1084:1142	As a result, at GA ≤ 28 weeks, we observed that protein content is higher in BM for multiples (1.53 vs. 1.29 g per 100 ml), lactose concentration is greater in BM for singletons (6.72 vs. 6.34 g per 100 ml) and GA results the most relevant factor influencing BM protein composition.
31337466	6	7	dep	greater	1011:1017	arg1	g					1055:1055	6.72 vs. 6.34 g	1041:1055	6.72 vs. 6.34 g per 100 ml	1041:1066	As a result, at GA ≤ 28 weeks, we observed that protein content is higher in BM for multiples (1.53 vs. 1.29 g per 100 ml), lactose concentration is greater in BM for singletons (6.72 vs. 6.34 g per 100 ml) and GA results the most relevant factor influencing BM protein composition.
31337466	3	8	theme	whole	412:416	arg1	pregnancies					418:428	the whole pregnancies	408:428	the whole pregnancies	408:428	Multiple births represent about 3% of the whole pregnancies; such neonates result more vulnerable than full-term newborns, due to lower GA and birth weight (BW) and the higher incidence of perinatal complications.
31337466	6	9	theme	GA	878:879	arg1	weeks					886:890	GA ≤ 28 weeks	878:890	GA ≤ 28 weeks	878:890	As a result, at GA ≤ 28 weeks, we observed that protein content is higher in BM for multiples (1.53 vs. 1.29 g per 100 ml), lactose concentration is greater in BM for singletons (6.72 vs. 6.34 g per 100 ml) and GA results the most relevant factor influencing BM protein composition.
31337466	5	10	theme	control	822:828	arg1	singletons					850:859	28 preterm singletons	839:859	28 preterm singletons	839:859	We collected and analyzed BM from mothers of 19 twins and 5 triplets showing GA < 33 weeks and BW < 1500 g, comparing it to a control group of 28 preterm singletons.
31337466	5	10	theme	control	822:828	arg1	group					830:834	a control group	820:834	a control group of 28 preterm singletons	820:859	We collected and analyzed BM from mothers of 19 twins and 5 triplets showing GA < 33 weeks and BW < 1500 g, comparing it to a control group of 28 preterm singletons.
31337466	1	11	theme	age	150:152	arg1	months					130:135	at least 6 months	119:135	at least 6 months of postnatal age	119:152	Exclusive breastfeeding is currently recommended until at least 6 months of postnatal age, due to maternal breast milk (BM) unique composition and beneficial properties.
31337466	9	12	theme	nutritional	1505:1515	arg1	strategies					1517:1526	nutritional strategies	1505:1526	nutritional strategies	1505:1526	These findings could help in optimizing nutritional strategies and improving BM individualized fortification.
31337466	4	13	from	studies	661:667	arg1	topic					677:681	this topic	672:681	this topic	672:681	Although an adequate nutrition is fundamental for twins and other multiples, studies on this topic are lacking.
31337466	1	14	theme	Exclusive	64:72	arg1	breastfeeding					74:86	Exclusive breastfeeding	64:86	Exclusive breastfeeding	64:86	Exclusive breastfeeding is currently recommended until at least 6 months of postnatal age, due to maternal breast milk (BM) unique composition and beneficial properties.
31337466	5	15	theme	triplets	756:763	arg1	mothers					730:736	mothers	730:736	mothers of 19 twins and 5 triplets showing GA < 33 weeks and BW < 1500 g	730:801	We collected and analyzed BM from mothers of 19 twins and 5 triplets showing GA < 33 weeks and BW < 1500 g, comparing it to a control group of 28 preterm singletons.
31337466	0	16	theme	Breast	0:5	arg1	Milk					7:10	Breast Milk	0:10	Breast Milk for Preterm Multiples: More Proteins, Less Lactose.	0:62	Breast Milk for Preterm Multiples: More Proteins, Less Lactose.
31337466	8	17	dep	lower	1426:1430	arg1	GA					1432:1433	GA	1432:1433	GA	1432:1433	BM from multiples shows a trophic and immunologic role, since these neonates often show lower GA and BW instead of singletons.
31337466	8	17	dep	lower	1426:1430	arg1	BW					1439:1440	BW	1439:1440	BW	1439:1440	BM from multiples shows a trophic and immunologic role, since these neonates often show lower GA and BW instead of singletons.
31337466	4	18	theme	other	644:648	arg1	multiples					650:658	other multiples	644:658	other multiples	644:658	Although an adequate nutrition is fundamental for twins and other multiples, studies on this topic are lacking.
31337466	3	19	theme	Multiple	370:377	arg1	births					379:384	Multiple births	370:384	Multiple births	370:384	Multiple births represent about 3% of the whole pregnancies; such neonates result more vulnerable than full-term newborns, due to lower GA and birth weight (BW) and the higher incidence of perinatal complications.
31337466	0	20	theme	Preterm	16:22	arg1	Multiples					24:32	Preterm Multiples	16:32	Preterm Multiples	16:32	Breast Milk for Preterm Multiples: More Proteins, Less Lactose.
31337466	5	21	theme	preterm	842:848	arg1	singletons					850:859	28 preterm singletons	839:859	28 preterm singletons	839:859	We collected and analyzed BM from mothers of 19 twins and 5 triplets showing GA < 33 weeks and BW < 1500 g, comparing it to a control group of 28 preterm singletons.
31337466	2	22	from	birth	299:303	arg1	GA					292:293	GA	292:293	GA	292:293	In fact, BM modifies itself according to gestational age (GA) at birth, adapting its composition to neonatal requests during lactation.
31337466	2	22	from	birth	299:303	arg1	age					287:289	gestational age	275:289	gestational age (GA) at birth	275:303	In fact, BM modifies itself according to gestational age (GA) at birth, adapting its composition to neonatal requests during lactation.
31337466	5	23	dep	GA	773:774	arg1	g					801:801	< 1500 g	794:801	< 1500 g	794:801	We collected and analyzed BM from mothers of 19 twins and 5 triplets showing GA < 33 weeks and BW < 1500 g, comparing it to a control group of 28 preterm singletons.
31337466	5	23	dep	GA	773:774	arg1	weeks					781:785	< 33 weeks	776:785	GA < 33 weeks	773:785	We collected and analyzed BM from mothers of 19 twins and 5 triplets showing GA < 33 weeks and BW < 1500 g, comparing it to a control group of 28 preterm singletons.
31337466	1	24	theme	maternal	162:169	arg1	composition					195:205	maternal breast milk (BM) unique composition	162:205	maternal breast milk (BM) unique composition	162:205	Exclusive breastfeeding is currently recommended until at least 6 months of postnatal age, due to maternal breast milk (BM) unique composition and beneficial properties.
31337466	9	25	theme	BM	1542:1543	arg1	fortification					1560:1572	BM individualized fortification	1542:1572	BM individualized fortification	1542:1572	These findings could help in optimizing nutritional strategies and improving BM individualized fortification.
31337466	7	26	from	growth	1280:1285	arg1	category					1328:1335	this vulnerable category	1312:1335	this vulnerable category	1312:1335	BM for multiples results higher in proteins and lower in lactose, if compared with singleton's samples; this could promote and sustain growth and organ development in this vulnerable category.
31337466	3	27	dep	lower	500:504	arg1	BW					527:528	BW	527:528	BW	527:528	Multiple births represent about 3% of the whole pregnancies; such neonates result more vulnerable than full-term newborns, due to lower GA and birth weight (BW) and the higher incidence of perinatal complications.
31337466	3	27	dep	lower	500:504	arg1	GA					506:507	GA	506:507	GA	506:507	Multiple births represent about 3% of the whole pregnancies; such neonates result more vulnerable than full-term newborns, due to lower GA and birth weight (BW) and the higher incidence of perinatal complications.
31337466	3	27	dep	lower	500:504	arg1	weight					519:524	birth weight	513:524	birth weight (BW)	513:529	Multiple births represent about 3% of the whole pregnancies; such neonates result more vulnerable than full-term newborns, due to lower GA and birth weight (BW) and the higher incidence of perinatal complications.
31337466	1	28	theme	breast	171:176	arg1	composition					195:205	maternal breast milk (BM) unique composition	162:205	maternal breast milk (BM) unique composition	162:205	Exclusive breastfeeding is currently recommended until at least 6 months of postnatal age, due to maternal breast milk (BM) unique composition and beneficial properties.
31337466	9	29	theme	individualized	1545:1558	arg1	fortification					1560:1572	BM individualized fortification	1542:1572	BM individualized fortification	1542:1572	These findings could help in optimizing nutritional strategies and improving BM individualized fortification.
31337466	3	30	theme	birth	513:517	arg1	BW					527:528	BW	527:528	BW	527:528	Multiple births represent about 3% of the whole pregnancies; such neonates result more vulnerable than full-term newborns, due to lower GA and birth weight (BW) and the higher incidence of perinatal complications.
31337466	3	30	theme	birth	513:517	arg1	weight					519:524	birth weight	513:524	birth weight (BW)	513:529	Multiple births represent about 3% of the whole pregnancies; such neonates result more vulnerable than full-term newborns, due to lower GA and birth weight (BW) and the higher incidence of perinatal complications.
31337466	1	31	theme	milk	178:181	arg1	composition					195:205	maternal breast milk (BM) unique composition	162:205	maternal breast milk (BM) unique composition	162:205	Exclusive breastfeeding is currently recommended until at least 6 months of postnatal age, due to maternal breast milk (BM) unique composition and beneficial properties.
31337466	6	32	dep	multiples	946:954	arg1	g					971:971	1.53 vs. 1.29 g	957:971	1.53 vs. 1.29 g per 100 ml	957:982	As a result, at GA ≤ 28 weeks, we observed that protein content is higher in BM for multiples (1.53 vs. 1.29 g per 100 ml), lactose concentration is greater in BM for singletons (6.72 vs. 6.34 g per 100 ml) and GA results the most relevant factor influencing BM protein composition.
31337466	7	33	theme	organ	1291:1295	arg1	development					1297:1307	organ development	1291:1307	organ development	1291:1307	BM for multiples results higher in proteins and lower in lactose, if compared with singleton's samples; this could promote and sustain growth and organ development in this vulnerable category.
31337466	5	34	theme	twins	744:748	arg1	mothers					730:736	mothers	730:736	mothers of 19 twins and 5 triplets showing GA < 33 weeks and BW < 1500 g	730:801	We collected and analyzed BM from mothers of 19 twins and 5 triplets showing GA < 33 weeks and BW < 1500 g, comparing it to a control group of 28 preterm singletons.
31337466	2	35	theme	neonatal	334:341	arg1	requests					343:350	neonatal requests	334:350	neonatal requests during lactation	334:367	In fact, BM modifies itself according to gestational age (GA) at birth, adapting its composition to neonatal requests during lactation.
31337466	7	36	from	development	1297:1307	arg1	category					1328:1335	this vulnerable category	1312:1335	this vulnerable category	1312:1335	BM for multiples results higher in proteins and lower in lactose, if compared with singleton's samples; this could promote and sustain growth and organ development in this vulnerable category.
31337466	3	37	theme	higher	539:544	arg1	incidence					546:554	the higher incidence	535:554	the higher incidence of perinatal complications	535:581	Multiple births represent about 3% of the whole pregnancies; such neonates result more vulnerable than full-term newborns, due to lower GA and birth weight (BW) and the higher incidence of perinatal complications.
31337466	3	38	theme	pregnancies	418:428	arg1	pregnancies					418:428	the whole pregnancies	408:428	the whole pregnancies	408:428	Multiple births represent about 3% of the whole pregnancies; such neonates result more vulnerable than full-term newborns, due to lower GA and birth weight (BW) and the higher incidence of perinatal complications.
31337466	3	38	theme	pregnancies	418:428	arg1	%					403:403	about 3%	396:403	about 3% of the whole pregnancies	396:428	Multiple births represent about 3% of the whole pregnancies; such neonates result more vulnerable than full-term newborns, due to lower GA and birth weight (BW) and the higher incidence of perinatal complications.
31337466	0	39	theme	More	35:38	arg1	Proteins					40:47	More Proteins	35:47	More Proteins	35:47	Breast Milk for Preterm Multiples: More Proteins, Less Lactose.
31337466	6	40	theme	lactose	986:992	arg1	concentration					994:1006	lactose concentration	986:1006	lactose concentration	986:1006	As a result, at GA ≤ 28 weeks, we observed that protein content is higher in BM for multiples (1.53 vs. 1.29 g per 100 ml), lactose concentration is greater in BM for singletons (6.72 vs. 6.34 g per 100 ml) and GA results the most relevant factor influencing BM protein composition.
31337466	5	41	from	mothers	730:736	arg1	BM					722:723	BM	722:723	BM from mothers of 19 twins and 5 triplets showing GA < 33 weeks and BW < 1500 g	722:801	We collected and analyzed BM from mothers of 19 twins and 5 triplets showing GA < 33 weeks and BW < 1500 g, comparing it to a control group of 28 preterm singletons.
31337466	8	42	theme	immunologic	1376:1386	arg1	role					1388:1391	a trophic and immunologic role	1362:1391	a trophic and immunologic role	1362:1391	BM from multiples shows a trophic and immunologic role, since these neonates often show lower GA and BW instead of singletons.
31337466	5	43	theme	<	794:794	arg1	g					801:801	< 1500 g	794:801	< 1500 g	794:801	We collected and analyzed BM from mothers of 19 twins and 5 triplets showing GA < 33 weeks and BW < 1500 g, comparing it to a control group of 28 preterm singletons.
31337466	1	44	theme	BM	184:185	arg1	composition					195:205	maternal breast milk (BM) unique composition	162:205	maternal breast milk (BM) unique composition	162:205	Exclusive breastfeeding is currently recommended until at least 6 months of postnatal age, due to maternal breast milk (BM) unique composition and beneficial properties.
31337466	8	45	from	multiples	1346:1354	arg1	BM					1338:1339	BM	1338:1339	BM from multiples	1338:1354	BM from multiples shows a trophic and immunologic role, since these neonates often show lower GA and BW instead of singletons.
31337466	3	46	theme	perinatal	559:567	arg1	complications					569:581	perinatal complications	559:581	perinatal complications	559:581	Multiple births represent about 3% of the whole pregnancies; such neonates result more vulnerable than full-term newborns, due to lower GA and birth weight (BW) and the higher incidence of perinatal complications.
31337466	2	47	theme	gestational	275:285	arg1	GA					292:293	GA	292:293	GA	292:293	In fact, BM modifies itself according to gestational age (GA) at birth, adapting its composition to neonatal requests during lactation.
31337466	2	47	theme	gestational	275:285	arg1	age					287:289	gestational age	275:289	gestational age (GA) at birth	275:303	In fact, BM modifies itself according to gestational age (GA) at birth, adapting its composition to neonatal requests during lactation.
31337466	7	48	theme	vulnerable	1317:1326	arg1	category					1328:1335	this vulnerable category	1312:1335	this vulnerable category	1312:1335	BM for multiples results higher in proteins and lower in lactose, if compared with singleton's samples; this could promote and sustain growth and organ development in this vulnerable category.
31337466	3	49	theme	complications	569:581	arg1	incidence					546:554	the higher incidence	535:554	the higher incidence of perinatal complications	535:581	Multiple births represent about 3% of the whole pregnancies; such neonates result more vulnerable than full-term newborns, due to lower GA and birth weight (BW) and the higher incidence of perinatal complications.
31337466	0	50	dep	Milk	7:10	arg1	Lactose					55:61	Lactose	55:61	Lactose	55:61	Breast Milk for Preterm Multiples: More Proteins, Less Lactose.
31337466	0	50	dep	Milk	7:10	arg1	Proteins					40:47	More Proteins	35:47	More Proteins	35:47	Breast Milk for Preterm Multiples: More Proteins, Less Lactose.
31337466	6	51	theme	≤	881:881	arg1	weeks					886:890	GA ≤ 28 weeks	878:890	GA ≤ 28 weeks	878:890	As a result, at GA ≤ 28 weeks, we observed that protein content is higher in BM for multiples (1.53 vs. 1.29 g per 100 ml), lactose concentration is greater in BM for singletons (6.72 vs. 6.34 g per 100 ml) and GA results the most relevant factor influencing BM protein composition.
31337466	4	52	theme	adequate	596:603	arg1	nutrition					605:613	an adequate nutrition	593:613	an adequate nutrition	593:613	Although an adequate nutrition is fundamental for twins and other multiples, studies on this topic are lacking.
31337466	4	52	theme	adequate	596:603	arg1	fundamental					618:628	fundamental	618:628	fundamental	618:628	Although an adequate nutrition is fundamental for twins and other multiples, studies on this topic are lacking.
31337466	1	53	theme	unique	188:193	arg1	composition					195:205	maternal breast milk (BM) unique composition	162:205	maternal breast milk (BM) unique composition	162:205	Exclusive breastfeeding is currently recommended until at least 6 months of postnatal age, due to maternal breast milk (BM) unique composition and beneficial properties.
31337466	6	54	theme	protein	1124:1130	arg1	composition					1132:1142	BM protein composition	1121:1142	BM protein composition	1121:1142	As a result, at GA ≤ 28 weeks, we observed that protein content is higher in BM for multiples (1.53 vs. 1.29 g per 100 ml), lactose concentration is greater in BM for singletons (6.72 vs. 6.34 g per 100 ml) and GA results the most relevant factor influencing BM protein composition.
31337466	5	55	theme	singletons	850:859	arg1	singletons					850:859	28 preterm singletons	839:859	28 preterm singletons	839:859	We collected and analyzed BM from mothers of 19 twins and 5 triplets showing GA < 33 weeks and BW < 1500 g, comparing it to a control group of 28 preterm singletons.
31337466	5	55	theme	singletons	850:859	arg1	group					830:834	a control group	820:834	a control group of 28 preterm singletons	820:859	We collected and analyzed BM from mothers of 19 twins and 5 triplets showing GA < 33 weeks and BW < 1500 g, comparing it to a control group of 28 preterm singletons.
31337466	6	56	theme	protein	910:916	arg1	content					918:924	protein content	910:924	protein content	910:924	As a result, at GA ≤ 28 weeks, we observed that protein content is higher in BM for multiples (1.53 vs. 1.29 g per 100 ml), lactose concentration is greater in BM for singletons (6.72 vs. 6.34 g per 100 ml) and GA results the most relevant factor influencing BM protein composition.
30575502	2	0	theme	aerobic	97:103	arg1	strain					138:143	A Gram-stain positive, aerobic, non-motile and short-rod-shaped strain	74:143	A Gram-stain positive, aerobic, non-motile and short-rod-shaped strain	74:143	A Gram-stain positive, aerobic, non-motile and short-rod-shaped strain, CFH S00084T, was isolated from a sediment sample of the Yellow River in Henan Province, China.
30575502	2	0	theme	aerobic	97:103	arg1	S00084T					150:156	CFH S00084T	146:156	CFH S00084T	146:156	A Gram-stain positive, aerobic, non-motile and short-rod-shaped strain, CFH S00084T, was isolated from a sediment sample of the Yellow River in Henan Province, China.
30575502	3	1	theme	DSM	491:493	arg1	strains					414:420	the type strains Microbacterium yannicii JCM 18959T and Microbacterium arthrosphaerae DSM 22421T	405:500	the type strains Microbacterium yannicii JCM 18959T and Microbacterium arthrosphaerae DSM 22421T	405:500	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CFH S00084T clustered within members of Microbacterium and was most closely related to the type strains Microbacterium yannicii JCM 18959T and Microbacterium arthrosphaerae DSM 22421T (98.97 % and 98.36 % similarity, respectively).
30575502	3	1	theme	DSM	491:493	arg1	22421T					495:500	Microbacterium arthrosphaerae DSM 22421T	461:500	Microbacterium arthrosphaerae DSM 22421T	461:500	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CFH S00084T clustered within members of Microbacterium and was most closely related to the type strains Microbacterium yannicii JCM 18959T and Microbacterium arthrosphaerae DSM 22421T (98.97 % and 98.36 % similarity, respectively).
30575502	11	2	theme	phylogenomic	1211:1222	arg1	analysis					1224:1231	The phylogenomic analysis	1207:1231	The phylogenomic analysis	1207:1231	The phylogenomic analysis provided evidence for clear phylogenetic divergence between strain CFH S00084T and its closely related type strains.
30575502	10	3	theme	dDDH	1194:1197	arg1	%					1188:1188	ANIb <75 %	1179:1188	ANIb <75 %	1179:1188	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between CFH S00084T and the other species of the genus Microbacterium were found to be low (ANIm <85 %, ANIb <75 % and dDDH <24 %).
30575502	10	3	theme	dDDH	1194:1197	arg1	%					1203:1203	dDDH <24 %	1194:1203	dDDH <24 %	1194:1203	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between CFH S00084T and the other species of the genus Microbacterium were found to be low (ANIm <85 %, ANIb <75 % and dDDH <24 %).
30575502	10	3	theme	dDDH	1194:1197	arg1	%					1176:1176	ANIm <85 %	1167:1176	ANIm <85 %	1167:1176	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between CFH S00084T and the other species of the genus Microbacterium were found to be low (ANIm <85 %, ANIb <75 % and dDDH <24 %).
30575502	10	3	theme	dDDH	1194:1197	arg1	low					1162:1164	low	1162:1164	low	1162:1164	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between CFH S00084T and the other species of the genus Microbacterium were found to be low (ANIm <85 %, ANIb <75 % and dDDH <24 %).
30575502	10	3	theme	dDDH	1194:1197	arg1	values					1068:1073	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values	989:1073	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between CFH S00084T and the other species of the genus Microbacterium	989:1143	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between CFH S00084T and the other species of the genus Microbacterium were found to be low (ANIm <85 %, ANIb <75 % and dDDH <24 %).
30575502	3	4	theme	98.36 	515:520	arg1	%					521:521	%	521:521	%	521:521	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CFH S00084T clustered within members of Microbacterium and was most closely related to the type strains Microbacterium yannicii JCM 18959T and Microbacterium arthrosphaerae DSM 22421T (98.97 % and 98.36 % similarity, respectively).
30575502	7	5	theme	CFH	783:785	arg1	S00084T					787:793	strain CFH S00084T	776:793	strain CFH S00084T	776:793	The menaquinones of strain CFH S00084T were MK-13, MK-12 and MK-11.
30575502	9	6	theme	70.5 mol	978:985	arg1	%					986:986	70.5 mol%	978:986	70.5 mol%	978:986	The genome of strain CFH S00084T was 4.03 Mbp with a G+C content of 70.5 mol%.
30575502	2	7	theme	short-rod-shaped	121:136	arg1	strain					138:143	A Gram-stain positive, aerobic, non-motile and short-rod-shaped strain	74:143	A Gram-stain positive, aerobic, non-motile and short-rod-shaped strain	74:143	A Gram-stain positive, aerobic, non-motile and short-rod-shaped strain, CFH S00084T, was isolated from a sediment sample of the Yellow River in Henan Province, China.
30575502	2	7	theme	short-rod-shaped	121:136	arg1	S00084T					150:156	CFH S00084T	146:156	CFH S00084T	146:156	A Gram-stain positive, aerobic, non-motile and short-rod-shaped strain, CFH S00084T, was isolated from a sediment sample of the Yellow River in Henan Province, China.
30575502	4	8	theme	0-3 	606:609	arg1	%					610:610	%	610:610	%	610:610	The strain grew optimally at 25-37 °C, at pH 7.0 and in 0-3 % (w/v) NaCl.
30575502	12	9	dep	properties	1397:1406	arg1	the					1353:1355	the	1353:1355	the	1353:1355	On the basis of the differential physiological properties, chemotaxonomic characteristics and low ANI and dDDH results, strain CFH S00084T is considered to represent a novel species for which the name Microbacteriumureisolvens sp.
30575502	12	9	dep	properties	1397:1406	arg1	basis					1357:1361	basis	1357:1361	basis	1357:1361	On the basis of the differential physiological properties, chemotaxonomic characteristics and low ANI and dDDH results, strain CFH S00084T is considered to represent a novel species for which the name Microbacteriumureisolvens sp.
30575502	12	10	theme	Microbacteriumureisolvens	1551:1575	arg1	sp					1577:1578	the name Microbacteriumureisolvens sp	1542:1578	the name Microbacteriumureisolvens sp	1542:1578	On the basis of the differential physiological properties, chemotaxonomic characteristics and low ANI and dDDH results, strain CFH S00084T is considered to represent a novel species for which the name Microbacteriumureisolvens sp.
30575502	12	11	theme	low	1444:1446	arg1	ANI					1448:1450	low ANI and dDDH results	1444:1467	ANI	1448:1450	On the basis of the differential physiological properties, chemotaxonomic characteristics and low ANI and dDDH results, strain CFH S00084T is considered to represent a novel species for which the name Microbacteriumureisolvens sp.
30575502	2	12	theme	positive	87:94	arg1	strain					138:143	A Gram-stain positive, aerobic, non-motile and short-rod-shaped strain	74:143	A Gram-stain positive, aerobic, non-motile and short-rod-shaped strain	74:143	A Gram-stain positive, aerobic, non-motile and short-rod-shaped strain, CFH S00084T, was isolated from a sediment sample of the Yellow River in Henan Province, China.
30575502	2	12	theme	positive	87:94	arg1	S00084T					150:156	CFH S00084T	146:156	CFH S00084T	146:156	A Gram-stain positive, aerobic, non-motile and short-rod-shaped strain, CFH S00084T, was isolated from a sediment sample of the Yellow River in Henan Province, China.
30575502	5	13	theme	major	628:632	arg1	rhamnose					657:664	rhamnose	657:664	rhamnose	657:664	The major whole-cell sugars were rhamnose and glucose.
30575502	5	13	theme	major	628:632	arg1	sugars					645:650	The major whole-cell sugars	624:650	The major whole-cell sugars	624:650	The major whole-cell sugars were rhamnose and glucose.
30575502	12	14	theme	physiological	1383:1395	arg1	properties					1397:1406	the differential physiological properties	1366:1406	the differential physiological properties	1366:1406	On the basis of the differential physiological properties, chemotaxonomic characteristics and low ANI and dDDH results, strain CFH S00084T is considered to represent a novel species for which the name Microbacteriumureisolvens sp.
30575502	10	15	dep	low	1162:1164	arg1	values					1068:1073	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values	989:1073	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between CFH S00084T and the other species of the genus Microbacterium	989:1143	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between CFH S00084T and the other species of the genus Microbacterium were found to be low (ANIm <85 %, ANIb <75 % and dDDH <24 %).
30575502	10	15	dep	low	1162:1164	arg1	low					1162:1164	low	1162:1164	low	1162:1164	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between CFH S00084T and the other species of the genus Microbacterium were found to be low (ANIm <85 %, ANIb <75 % and dDDH <24 %).
30575502	10	15	dep	low	1162:1164	arg1	%					1176:1176	ANIm <85 %	1167:1176	ANIm <85 %	1167:1176	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between CFH S00084T and the other species of the genus Microbacterium were found to be low (ANIm <85 %, ANIb <75 % and dDDH <24 %).
30575502	10	15	dep	low	1162:1164	arg1	%					1203:1203	dDDH <24 %	1194:1203	dDDH <24 %	1194:1203	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between CFH S00084T and the other species of the genus Microbacterium were found to be low (ANIm <85 %, ANIb <75 % and dDDH <24 %).
30575502	10	15	dep	low	1162:1164	arg1	%					1188:1188	ANIb <75 %	1179:1188	ANIb <75 %	1179:1188	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between CFH S00084T and the other species of the genus Microbacterium were found to be low (ANIm <85 %, ANIb <75 % and dDDH <24 %).
30575502	2	16	from	China	234:238	arg1	sample					188:193	a sediment sample	177:193	a sediment sample of the Yellow River in Henan Province, China	177:238	A Gram-stain positive, aerobic, non-motile and short-rod-shaped strain, CFH S00084T, was isolated from a sediment sample of the Yellow River in Henan Province, China.
30575502	11	17	theme	strain	1293:1298	arg1	S00084T					1304:1310	strain CFH S00084T	1293:1310	strain CFH S00084T	1293:1310	The phylogenomic analysis provided evidence for clear phylogenetic divergence between strain CFH S00084T and its closely related type strains.
30575502	11	18	theme	CFH	1300:1302	arg1	S00084T					1304:1310	strain CFH S00084T	1293:1310	strain CFH S00084T	1293:1310	The phylogenomic analysis provided evidence for clear phylogenetic divergence between strain CFH S00084T and its closely related type strains.
30575502	2	19	theme	River	209:213	arg1	sample					188:193	a sediment sample	177:193	a sediment sample of the Yellow River in Henan Province, China	177:238	A Gram-stain positive, aerobic, non-motile and short-rod-shaped strain, CFH S00084T, was isolated from a sediment sample of the Yellow River in Henan Province, China.
30575502	3	20	theme	rRNA	276:279	arg1	sequences					286:294	16S rRNA gene sequences	272:294	16S rRNA gene sequences	272:294	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CFH S00084T clustered within members of Microbacterium and was most closely related to the type strains Microbacterium yannicii JCM 18959T and Microbacterium arthrosphaerae DSM 22421T (98.97 % and 98.36 % similarity, respectively).
30575502	9	21	theme	CFH	931:933	arg1	S00084T					935:941	strain CFH S00084T	924:941	strain CFH S00084T	924:941	The genome of strain CFH S00084T was 4.03 Mbp with a G+C content of 70.5 mol%.
30575502	14	22	theme	=KCTC	1631:1635	arg1	103157T					1648:1654	=KCTC 39802T=DSM 103157T	1631:1654	=KCTC 39802T=DSM 103157T	1631:1654	The type strain is CFH S00084T (=KCTC 39802T=DSM 103157T).
30575502	14	22	theme	=KCTC	1631:1635	arg1	S00084T					1622:1628	CFH S00084T	1618:1628	CFH S00084T (=KCTC 39802T=DSM 103157T)	1618:1655	The type strain is CFH S00084T (=KCTC 39802T=DSM 103157T).
30575502	3	23	theme	CFH	318:320	arg1	S00084T					322:328	strain CFH S00084T	311:328	strain CFH S00084T	311:328	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CFH S00084T clustered within members of Microbacterium and was most closely related to the type strains Microbacterium yannicii JCM 18959T and Microbacterium arthrosphaerae DSM 22421T (98.97 % and 98.36 % similarity, respectively).
30575502	1	24	theme	Yellow	53:58	arg1	sample					66:71	a Yellow River sample	51:71	a Yellow River sample	51:71	nov., isolated from a Yellow River sample.
30575502	12	25	theme	CFH	1477:1479	arg1	S00084T					1481:1487	strain CFH S00084T	1470:1487	strain CFH S00084T	1470:1487	On the basis of the differential physiological properties, chemotaxonomic characteristics and low ANI and dDDH results, strain CFH S00084T is considered to represent a novel species for which the name Microbacteriumureisolvens sp.
30575502	10	26	theme	Microbacterium	1130:1143	arg1	S00084T					1087:1093	CFH S00084T	1083:1093	CFH S00084T	1083:1093	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between CFH S00084T and the other species of the genus Microbacterium were found to be low (ANIm <85 %, ANIb <75 % and dDDH <24 %).
30575502	10	26	theme	Microbacterium	1130:1143	arg1	species					1109:1115	the other species	1099:1115	the other species of the genus Microbacterium	1099:1143	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between CFH S00084T and the other species of the genus Microbacterium were found to be low (ANIm <85 %, ANIb <75 % and dDDH <24 %).
30575502	3	27	dep	Microbacterium	461:474	arg1	arthrosphaerae					476:489	arthrosphaerae	476:489	arthrosphaerae	476:489	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CFH S00084T clustered within members of Microbacterium and was most closely related to the type strains Microbacterium yannicii JCM 18959T and Microbacterium arthrosphaerae DSM 22421T (98.97 % and 98.36 % similarity, respectively).
30575502	10	28	theme	nucleotide	1001:1010	arg1	ANI					1022:1024	ANI	1022:1024	ANI	1022:1024	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between CFH S00084T and the other species of the genus Microbacterium were found to be low (ANIm <85 %, ANIb <75 % and dDDH <24 %).
30575502	10	28	theme	nucleotide	1001:1010	arg1	identity					1012:1019	average nucleotide identity	993:1019	average nucleotide identity (ANI)	993:1025	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between CFH S00084T and the other species of the genus Microbacterium were found to be low (ANIm <85 %, ANIb <75 % and dDDH <24 %).
30575502	12	29	theme	dDDH	1456:1459	arg1	results					1461:1467	low ANI and dDDH results	1444:1467	results	1461:1467	On the basis of the differential physiological properties, chemotaxonomic characteristics and low ANI and dDDH results, strain CFH S00084T is considered to represent a novel species for which the name Microbacteriumureisolvens sp.
30575502	3	30	theme	Microbacterium	422:435	arg1	strains					414:420	the type strains Microbacterium yannicii JCM 18959T and Microbacterium arthrosphaerae DSM 22421T	405:500	the type strains Microbacterium yannicii JCM 18959T and Microbacterium arthrosphaerae DSM 22421T	405:500	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CFH S00084T clustered within members of Microbacterium and was most closely related to the type strains Microbacterium yannicii JCM 18959T and Microbacterium arthrosphaerae DSM 22421T (98.97 % and 98.36 % similarity, respectively).
30575502	3	30	theme	Microbacterium	422:435	arg1	18959T					450:455	Microbacterium yannicii JCM 18959T	422:455	Microbacterium yannicii JCM 18959T	422:455	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CFH S00084T clustered within members of Microbacterium and was most closely related to the type strains Microbacterium yannicii JCM 18959T and Microbacterium arthrosphaerae DSM 22421T (98.97 % and 98.36 % similarity, respectively).
30575502	14	31	theme	CFH	1618:1620	arg1	103157T					1648:1654	=KCTC 39802T=DSM 103157T	1631:1654	=KCTC 39802T=DSM 103157T	1631:1654	The type strain is CFH S00084T (=KCTC 39802T=DSM 103157T).
30575502	14	31	theme	CFH	1618:1620	arg1	strain					1608:1613	The type strain	1599:1613	The type strain	1599:1613	The type strain is CFH S00084T (=KCTC 39802T=DSM 103157T).
30575502	14	31	theme	CFH	1618:1620	arg1	S00084T					1622:1628	CFH S00084T	1618:1628	CFH S00084T (=KCTC 39802T=DSM 103157T)	1618:1655	The type strain is CFH S00084T (=KCTC 39802T=DSM 103157T).
30575502	9	32	theme	%	986:986	arg1	G+C content					963:973	a G+C content	961:973	a G+C content of 70.5 mol%	961:986	The genome of strain CFH S00084T was 4.03 Mbp with a G+C content of 70.5 mol%.
30575502	11	33	theme	phylogenetic	1261:1272	arg1	divergence					1274:1283	clear phylogenetic divergence	1255:1283	clear phylogenetic divergence between strain CFH S00084T and its closely related type strains	1255:1347	The phylogenomic analysis provided evidence for clear phylogenetic divergence between strain CFH S00084T and its closely related type strains.
30575502	3	34	theme	type	409:412	arg1	strains					414:420	the type strains Microbacterium yannicii JCM 18959T and Microbacterium arthrosphaerae DSM 22421T	405:500	the type strains Microbacterium yannicii JCM 18959T and Microbacterium arthrosphaerae DSM 22421T	405:500	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CFH S00084T clustered within members of Microbacterium and was most closely related to the type strains Microbacterium yannicii JCM 18959T and Microbacterium arthrosphaerae DSM 22421T (98.97 % and 98.36 % similarity, respectively).
30575502	3	34	theme	type	409:412	arg1	22421T					495:500	Microbacterium arthrosphaerae DSM 22421T	461:500	Microbacterium arthrosphaerae DSM 22421T	461:500	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CFH S00084T clustered within members of Microbacterium and was most closely related to the type strains Microbacterium yannicii JCM 18959T and Microbacterium arthrosphaerae DSM 22421T (98.97 % and 98.36 % similarity, respectively).
30575502	3	34	theme	type	409:412	arg1	18959T					450:455	Microbacterium yannicii JCM 18959T	422:455	Microbacterium yannicii JCM 18959T	422:455	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CFH S00084T clustered within members of Microbacterium and was most closely related to the type strains Microbacterium yannicii JCM 18959T and Microbacterium arthrosphaerae DSM 22421T (98.97 % and 98.36 % similarity, respectively).
30575502	3	35	dep	related	394:400	arg1	similarity					523:532	98.36 % similarity	515:532	98.36 % similarity	515:532	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CFH S00084T clustered within members of Microbacterium and was most closely related to the type strains Microbacterium yannicii JCM 18959T and Microbacterium arthrosphaerae DSM 22421T (98.97 % and 98.36 % similarity, respectively).
30575502	3	35	dep	related	394:400	arg1	%					509:509	98.97 %	503:509	98.97 %	503:509	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CFH S00084T clustered within members of Microbacterium and was most closely related to the type strains Microbacterium yannicii JCM 18959T and Microbacterium arthrosphaerae DSM 22421T (98.97 % and 98.36 % similarity, respectively).
30575502	8	36	theme	fatty	834:838	arg1	acids					840:844	The major fatty acids	824:844	The major fatty acids detected	824:853	The major fatty acids detected were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
30575502	8	36	theme	fatty	834:838	arg1	anteiso-C15 					860:871	anteiso-C15 	860:871	anteiso-C15 	860:871	The major fatty acids detected were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
30575502	3	37	theme	Microbacterium	358:371	arg1	members					347:353	members	347:353	members of Microbacterium	347:371	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CFH S00084T clustered within members of Microbacterium and was most closely related to the type strains Microbacterium yannicii JCM 18959T and Microbacterium arthrosphaerae DSM 22421T (98.97 % and 98.36 % similarity, respectively).
30575502	2	38	theme	CFH	146:148	arg1	strain					138:143	A Gram-stain positive, aerobic, non-motile and short-rod-shaped strain	74:143	A Gram-stain positive, aerobic, non-motile and short-rod-shaped strain	74:143	A Gram-stain positive, aerobic, non-motile and short-rod-shaped strain, CFH S00084T, was isolated from a sediment sample of the Yellow River in Henan Province, China.
30575502	2	38	theme	CFH	146:148	arg1	S00084T					150:156	CFH S00084T	146:156	CFH S00084T	146:156	A Gram-stain positive, aerobic, non-motile and short-rod-shaped strain, CFH S00084T, was isolated from a sediment sample of the Yellow River in Henan Province, China.
30575502	3	39	theme	JCM	446:448	arg1	strains					414:420	the type strains Microbacterium yannicii JCM 18959T and Microbacterium arthrosphaerae DSM 22421T	405:500	the type strains Microbacterium yannicii JCM 18959T and Microbacterium arthrosphaerae DSM 22421T	405:500	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CFH S00084T clustered within members of Microbacterium and was most closely related to the type strains Microbacterium yannicii JCM 18959T and Microbacterium arthrosphaerae DSM 22421T (98.97 % and 98.36 % similarity, respectively).
30575502	3	39	theme	JCM	446:448	arg1	18959T					450:455	Microbacterium yannicii JCM 18959T	422:455	Microbacterium yannicii JCM 18959T	422:455	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CFH S00084T clustered within members of Microbacterium and was most closely related to the type strains Microbacterium yannicii JCM 18959T and Microbacterium arthrosphaerae DSM 22421T (98.97 % and 98.36 % similarity, respectively).
30575502	10	40	theme	hybridization	1047:1059	arg1	%					1188:1188	ANIb <75 %	1179:1188	ANIb <75 %	1179:1188	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between CFH S00084T and the other species of the genus Microbacterium were found to be low (ANIm <85 %, ANIb <75 % and dDDH <24 %).
30575502	10	40	theme	hybridization	1047:1059	arg1	%					1176:1176	ANIm <85 %	1167:1176	ANIm <85 %	1167:1176	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between CFH S00084T and the other species of the genus Microbacterium were found to be low (ANIm <85 %, ANIb <75 % and dDDH <24 %).
30575502	10	40	theme	hybridization	1047:1059	arg1	%					1203:1203	dDDH <24 %	1194:1203	dDDH <24 %	1194:1203	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between CFH S00084T and the other species of the genus Microbacterium were found to be low (ANIm <85 %, ANIb <75 % and dDDH <24 %).
30575502	10	40	theme	hybridization	1047:1059	arg1	low					1162:1164	low	1162:1164	low	1162:1164	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between CFH S00084T and the other species of the genus Microbacterium were found to be low (ANIm <85 %, ANIb <75 % and dDDH <24 %).
30575502	10	40	theme	hybridization	1047:1059	arg1	values					1068:1073	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values	989:1073	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between CFH S00084T and the other species of the genus Microbacterium	989:1143	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between CFH S00084T and the other species of the genus Microbacterium were found to be low (ANIm <85 %, ANIb <75 % and dDDH <24 %).
30575502	3	41	dep	strains	414:420	arg1	strains					414:420	the type strains Microbacterium yannicii JCM 18959T and Microbacterium arthrosphaerae DSM 22421T	405:500	the type strains Microbacterium yannicii JCM 18959T and Microbacterium arthrosphaerae DSM 22421T	405:500	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CFH S00084T clustered within members of Microbacterium and was most closely related to the type strains Microbacterium yannicii JCM 18959T and Microbacterium arthrosphaerae DSM 22421T (98.97 % and 98.36 % similarity, respectively).
30575502	3	41	dep	strains	414:420	arg1	22421T					495:500	Microbacterium arthrosphaerae DSM 22421T	461:500	Microbacterium arthrosphaerae DSM 22421T	461:500	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CFH S00084T clustered within members of Microbacterium and was most closely related to the type strains Microbacterium yannicii JCM 18959T and Microbacterium arthrosphaerae DSM 22421T (98.97 % and 98.36 % similarity, respectively).
30575502	3	41	dep	strains	414:420	arg1	18959T					450:455	Microbacterium yannicii JCM 18959T	422:455	Microbacterium yannicii JCM 18959T	422:455	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CFH S00084T clustered within members of Microbacterium and was most closely related to the type strains Microbacterium yannicii JCM 18959T and Microbacterium arthrosphaerae DSM 22421T (98.97 % and 98.36 % similarity, respectively).
30575502	8	42	dep	anteiso-C15 	860:871	arg1	iso-C16 					897:904	iso-C16 	897:904	iso-C16 	897:904	The major fatty acids detected were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
30575502	8	42	dep	anteiso-C15 	860:871	arg1	 0					873:874	 0	873:874	 0	873:874	The major fatty acids detected were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
30575502	8	42	dep	anteiso-C15 	860:871	arg1	 0					890:891	 0	890:891	 0	890:891	The major fatty acids detected were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
30575502	8	42	dep	anteiso-C15 	860:871	arg1	 0					906:907	 0	906:907	 0	906:907	The major fatty acids detected were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
30575502	9	43	theme	strain	924:929	arg1	S00084T					935:941	strain CFH S00084T	924:941	strain CFH S00084T	924:941	The genome of strain CFH S00084T was 4.03 Mbp with a G+C content of 70.5 mol%.
30575502	10	44	theme	digital	1031:1037	arg1	dDDH					1062:1065	dDDH	1062:1065	dDDH	1062:1065	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between CFH S00084T and the other species of the genus Microbacterium were found to be low (ANIm <85 %, ANIb <75 % and dDDH <24 %).
30575502	10	44	theme	digital	1031:1037	arg1	hybridization					1047:1059	digital DNA-DNA hybridization	1031:1059	digital DNA-DNA hybridization (dDDH)	1031:1066	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between CFH S00084T and the other species of the genus Microbacterium were found to be low (ANIm <85 %, ANIb <75 % and dDDH <24 %).
30575502	11	45	theme	type	1336:1339	arg1	strains					1341:1347	its closely related type strains	1316:1347	its closely related type strains	1316:1347	The phylogenomic analysis provided evidence for clear phylogenetic divergence between strain CFH S00084T and its closely related type strains.
30575502	7	46	theme	S00084T	787:793	arg1	menaquinones					760:771	The menaquinones	756:771	The menaquinones of strain CFH S00084T	756:793	The menaquinones of strain CFH S00084T were MK-13, MK-12 and MK-11.
30575502	7	46	theme	S00084T	787:793	arg1	MK-13					800:804	MK-13	800:804	MK-13	800:804	The menaquinones of strain CFH S00084T were MK-13, MK-12 and MK-11.
30575502	12	47	theme	name	1546:1549	arg1	sp					1577:1578	the name Microbacteriumureisolvens sp	1542:1578	the name Microbacteriumureisolvens sp	1542:1578	On the basis of the differential physiological properties, chemotaxonomic characteristics and low ANI and dDDH results, strain CFH S00084T is considered to represent a novel species for which the name Microbacteriumureisolvens sp.
30575502	7	48	theme	strain	776:781	arg1	S00084T					787:793	strain CFH S00084T	776:793	strain CFH S00084T	776:793	The menaquinones of strain CFH S00084T were MK-13, MK-12 and MK-11.
30575502	6	49	theme	cell-wall	683:691	arg1	peptidoglycan					693:705	The cell-wall peptidoglycan	679:705	The cell-wall peptidoglycan	679:705	The cell-wall peptidoglycan mainly contained glycine, alanine and ornithine.
30575502	3	50	theme	%	521:521	arg1	similarity					523:532	98.36 % similarity	515:532	98.36 % similarity	515:532	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CFH S00084T clustered within members of Microbacterium and was most closely related to the type strains Microbacterium yannicii JCM 18959T and Microbacterium arthrosphaerae DSM 22421T (98.97 % and 98.36 % similarity, respectively).
30575502	3	51	theme	Phylogenetic	241:252	arg1	analysis					254:261	Phylogenetic analysis	241:261	Phylogenetic analysis based on 16S rRNA gene sequences	241:294	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CFH S00084T clustered within members of Microbacterium and was most closely related to the type strains Microbacterium yannicii JCM 18959T and Microbacterium arthrosphaerae DSM 22421T (98.97 % and 98.36 % similarity, respectively).
30575502	10	52	theme	other	1103:1107	arg1	species					1109:1115	the other species	1099:1115	the other species of the genus Microbacterium	1099:1143	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between CFH S00084T and the other species of the genus Microbacterium were found to be low (ANIm <85 %, ANIb <75 % and dDDH <24 %).
30575502	2	53	theme	Yellow	202:207	arg1	River					209:213	the Yellow River	198:213	the Yellow River in Henan Province, China	198:238	A Gram-stain positive, aerobic, non-motile and short-rod-shaped strain, CFH S00084T, was isolated from a sediment sample of the Yellow River in Henan Province, China.
30575502	2	54	theme	Gram-stain	76:85	arg1	strain					138:143	A Gram-stain positive, aerobic, non-motile and short-rod-shaped strain	74:143	A Gram-stain positive, aerobic, non-motile and short-rod-shaped strain	74:143	A Gram-stain positive, aerobic, non-motile and short-rod-shaped strain, CFH S00084T, was isolated from a sediment sample of the Yellow River in Henan Province, China.
30575502	2	54	theme	Gram-stain	76:85	arg1	S00084T					150:156	CFH S00084T	146:156	CFH S00084T	146:156	A Gram-stain positive, aerobic, non-motile and short-rod-shaped strain, CFH S00084T, was isolated from a sediment sample of the Yellow River in Henan Province, China.
30575502	12	55	theme	chemotaxonomic	1409:1422	arg1	characteristics					1424:1438	chemotaxonomic characteristics	1409:1438	chemotaxonomic characteristics	1409:1438	On the basis of the differential physiological properties, chemotaxonomic characteristics and low ANI and dDDH results, strain CFH S00084T is considered to represent a novel species for which the name Microbacteriumureisolvens sp.
30575502	3	56	theme	16S	272:274	arg1	sequences					286:294	16S rRNA gene sequences	272:294	16S rRNA gene sequences	272:294	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CFH S00084T clustered within members of Microbacterium and was most closely related to the type strains Microbacterium yannicii JCM 18959T and Microbacterium arthrosphaerae DSM 22421T (98.97 % and 98.36 % similarity, respectively).
30575502	12	57	theme	novel	1518:1522	arg1	species					1524:1530	a novel species	1516:1530	a novel species for which the name Microbacteriumureisolvens sp	1516:1578	On the basis of the differential physiological properties, chemotaxonomic characteristics and low ANI and dDDH results, strain CFH S00084T is considered to represent a novel species for which the name Microbacteriumureisolvens sp.
30575502	10	58	theme	CFH	1083:1085	arg1	S00084T					1087:1093	CFH S00084T	1083:1093	CFH S00084T	1083:1093	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between CFH S00084T and the other species of the genus Microbacterium were found to be low (ANIm <85 %, ANIb <75 % and dDDH <24 %).
30575502	9	59	with	Mbp	952:954	arg1	G+C content					963:973	a G+C content	961:973	a G+C content of 70.5 mol%	961:986	The genome of strain CFH S00084T was 4.03 Mbp with a G+C content of 70.5 mol%.
30575502	12	60	theme	differential	1370:1381	arg1	properties					1397:1406	the differential physiological properties	1366:1406	the differential physiological properties	1366:1406	On the basis of the differential physiological properties, chemotaxonomic characteristics and low ANI and dDDH results, strain CFH S00084T is considered to represent a novel species for which the name Microbacteriumureisolvens sp.
30575502	4	61	theme	%	610:610	arg1	NaCl					618:621	0-3 % (w/v) NaCl	606:621	0-3 % (w/v) NaCl	606:621	The strain grew optimally at 25-37 °C, at pH 7.0 and in 0-3 % (w/v) NaCl.
30575502	11	62	theme	related	1328:1334	arg1	strains					1341:1347	its closely related type strains	1316:1347	its closely related type strains	1316:1347	The phylogenomic analysis provided evidence for clear phylogenetic divergence between strain CFH S00084T and its closely related type strains.
30575502	14	63	theme	39802T=DSM	1637:1646	arg1	103157T					1648:1654	=KCTC 39802T=DSM 103157T	1631:1654	=KCTC 39802T=DSM 103157T	1631:1654	The type strain is CFH S00084T (=KCTC 39802T=DSM 103157T).
30575502	14	63	theme	39802T=DSM	1637:1646	arg1	S00084T					1622:1628	CFH S00084T	1618:1628	CFH S00084T (=KCTC 39802T=DSM 103157T)	1618:1655	The type strain is CFH S00084T (=KCTC 39802T=DSM 103157T).
30575502	3	64	theme	gene	281:284	arg1	sequences					286:294	16S rRNA gene sequences	272:294	16S rRNA gene sequences	272:294	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CFH S00084T clustered within members of Microbacterium and was most closely related to the type strains Microbacterium yannicii JCM 18959T and Microbacterium arthrosphaerae DSM 22421T (98.97 % and 98.36 % similarity, respectively).
30575502	9	65	theme	S00084T	935:941	arg1	genome					914:919	The genome	910:919	The genome of strain CFH S00084T	910:941	The genome of strain CFH S00084T was 4.03 Mbp with a G+C content of 70.5 mol%.
30575502	9	65	theme	S00084T	935:941	arg1	Mbp					952:954	4.03 Mbp	947:954	4.03 Mbp	947:954	The genome of strain CFH S00084T was 4.03 Mbp with a G+C content of 70.5 mol%.
30575502	5	66	theme	whole-cell	634:643	arg1	rhamnose					657:664	rhamnose	657:664	rhamnose	657:664	The major whole-cell sugars were rhamnose and glucose.
30575502	5	66	theme	whole-cell	634:643	arg1	sugars					645:650	The major whole-cell sugars	624:650	The major whole-cell sugars	624:650	The major whole-cell sugars were rhamnose and glucose.
30575502	1	67	theme	River	60:64	arg1	sample					66:71	a Yellow River sample	51:71	a Yellow River sample	51:71	nov., isolated from a Yellow River sample.
30575502	2	68	from	sample	188:193	arg1	China					234:238	China	234:238	China	234:238	A Gram-stain positive, aerobic, non-motile and short-rod-shaped strain, CFH S00084T, was isolated from a sediment sample of the Yellow River in Henan Province, China.
30575502	3	69	theme	strain	311:316	arg1	S00084T					322:328	strain CFH S00084T	311:328	strain CFH S00084T	311:328	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CFH S00084T clustered within members of Microbacterium and was most closely related to the type strains Microbacterium yannicii JCM 18959T and Microbacterium arthrosphaerae DSM 22421T (98.97 % and 98.36 % similarity, respectively).
30575502	10	70	theme	genus	1124:1128	arg1	Microbacterium					1130:1143	the genus Microbacterium	1120:1143	the genus Microbacterium	1120:1143	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between CFH S00084T and the other species of the genus Microbacterium were found to be low (ANIm <85 %, ANIb <75 % and dDDH <24 %).
30575502	2	71	attach	isolated	163:170	arg1	sample					188:193	a sediment sample	177:193	a sediment sample of the Yellow River in Henan Province, China	177:238	A Gram-stain positive, aerobic, non-motile and short-rod-shaped strain, CFH S00084T, was isolated from a sediment sample of the Yellow River in Henan Province, China.
30575502	2	71	attach	isolated	163:170	arg2	strain					138:143	A Gram-stain positive, aerobic, non-motile and short-rod-shaped strain	74:143	A Gram-stain positive, aerobic, non-motile and short-rod-shaped strain	74:143	A Gram-stain positive, aerobic, non-motile and short-rod-shaped strain, CFH S00084T, was isolated from a sediment sample of the Yellow River in Henan Province, China.
30575502	2	71	attach	isolated	163:170	arg2	S00084T					150:156	CFH S00084T	146:156	CFH S00084T	146:156	A Gram-stain positive, aerobic, non-motile and short-rod-shaped strain, CFH S00084T, was isolated from a sediment sample of the Yellow River in Henan Province, China.
30575502	10	72	theme	average	993:999	arg1	ANI					1022:1024	ANI	1022:1024	ANI	1022:1024	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between CFH S00084T and the other species of the genus Microbacterium were found to be low (ANIm <85 %, ANIb <75 % and dDDH <24 %).
30575502	10	72	theme	average	993:999	arg1	identity					1012:1019	average nucleotide identity	993:1019	average nucleotide identity (ANI)	993:1025	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between CFH S00084T and the other species of the genus Microbacterium were found to be low (ANIm <85 %, ANIb <75 % and dDDH <24 %).
30575502	2	73	from	River	209:213	arg1	China					234:238	China	234:238	China	234:238	A Gram-stain positive, aerobic, non-motile and short-rod-shaped strain, CFH S00084T, was isolated from a sediment sample of the Yellow River in Henan Province, China.
30575502	11	74	theme	clear	1255:1259	arg1	divergence					1274:1283	clear phylogenetic divergence	1255:1283	clear phylogenetic divergence between strain CFH S00084T and its closely related type strains	1255:1347	The phylogenomic analysis provided evidence for clear phylogenetic divergence between strain CFH S00084T and its closely related type strains.
30575502	12	75	theme	strain	1470:1475	arg1	S00084T					1481:1487	strain CFH S00084T	1470:1487	strain CFH S00084T	1470:1487	On the basis of the differential physiological properties, chemotaxonomic characteristics and low ANI and dDDH results, strain CFH S00084T is considered to represent a novel species for which the name Microbacteriumureisolvens sp.
30575502	6	76	contain	contained	714:722	arg2	ornithine					745:753	ornithine	745:753	ornithine	745:753	The cell-wall peptidoglycan mainly contained glycine, alanine and ornithine.
30575502	6	76	contain	contained	714:722	arg2	alanine					733:739	alanine	733:739	alanine	733:739	The cell-wall peptidoglycan mainly contained glycine, alanine and ornithine.
30575502	6	76	contain	contained	714:722	arg1	peptidoglycan					693:705	The cell-wall peptidoglycan	679:705	The cell-wall peptidoglycan	679:705	The cell-wall peptidoglycan mainly contained glycine, alanine and ornithine.
30575502	6	76	contain	contained	714:722	arg2	glycine					724:730	glycine	724:730	glycine	724:730	The cell-wall peptidoglycan mainly contained glycine, alanine and ornithine.
30575502	14	77	theme	type	1603:1606	arg1	strain					1608:1613	The type strain	1599:1613	The type strain	1599:1613	The type strain is CFH S00084T (=KCTC 39802T=DSM 103157T).
30575502	14	77	theme	type	1603:1606	arg1	S00084T					1622:1628	CFH S00084T	1618:1628	CFH S00084T (=KCTC 39802T=DSM 103157T)	1618:1655	The type strain is CFH S00084T (=KCTC 39802T=DSM 103157T).
30575502	8	78	theme	major	828:832	arg1	acids					840:844	The major fatty acids	824:844	The major fatty acids detected	824:853	The major fatty acids detected were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
30575502	8	78	theme	major	828:832	arg1	anteiso-C15 					860:871	anteiso-C15 	860:871	anteiso-C15 	860:871	The major fatty acids detected were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
30575502	2	79	theme	sediment	179:186	arg1	sample					188:193	a sediment sample	177:193	a sediment sample of the Yellow River in Henan Province, China	177:238	A Gram-stain positive, aerobic, non-motile and short-rod-shaped strain, CFH S00084T, was isolated from a sediment sample of the Yellow River in Henan Province, China.
30575502	10	80	theme	ANIm	1167:1170	arg1	%					1188:1188	ANIb <75 %	1179:1188	ANIb <75 %	1179:1188	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between CFH S00084T and the other species of the genus Microbacterium were found to be low (ANIm <85 %, ANIb <75 % and dDDH <24 %).
30575502	10	80	theme	ANIm	1167:1170	arg1	%					1176:1176	ANIm <85 %	1167:1176	ANIm <85 %	1167:1176	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between CFH S00084T and the other species of the genus Microbacterium were found to be low (ANIm <85 %, ANIb <75 % and dDDH <24 %).
30575502	10	80	theme	ANIm	1167:1170	arg1	%					1203:1203	dDDH <24 %	1194:1203	dDDH <24 %	1194:1203	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between CFH S00084T and the other species of the genus Microbacterium were found to be low (ANIm <85 %, ANIb <75 % and dDDH <24 %).
30575502	10	80	theme	ANIm	1167:1170	arg1	low					1162:1164	low	1162:1164	low	1162:1164	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between CFH S00084T and the other species of the genus Microbacterium were found to be low (ANIm <85 %, ANIb <75 % and dDDH <24 %).
30575502	10	80	theme	ANIm	1167:1170	arg1	values					1068:1073	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values	989:1073	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between CFH S00084T and the other species of the genus Microbacterium	989:1143	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between CFH S00084T and the other species of the genus Microbacterium were found to be low (ANIm <85 %, ANIb <75 % and dDDH <24 %).
30575502	3	81	theme	Microbacterium	461:474	arg1	strains					414:420	the type strains Microbacterium yannicii JCM 18959T and Microbacterium arthrosphaerae DSM 22421T	405:500	the type strains Microbacterium yannicii JCM 18959T and Microbacterium arthrosphaerae DSM 22421T	405:500	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CFH S00084T clustered within members of Microbacterium and was most closely related to the type strains Microbacterium yannicii JCM 18959T and Microbacterium arthrosphaerae DSM 22421T (98.97 % and 98.36 % similarity, respectively).
30575502	3	81	theme	Microbacterium	461:474	arg1	22421T					495:500	Microbacterium arthrosphaerae DSM 22421T	461:500	Microbacterium arthrosphaerae DSM 22421T	461:500	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CFH S00084T clustered within members of Microbacterium and was most closely related to the type strains Microbacterium yannicii JCM 18959T and Microbacterium arthrosphaerae DSM 22421T (98.97 % and 98.36 % similarity, respectively).
30575502	10	82	theme	identity	1012:1019	arg1	%					1188:1188	ANIb <75 %	1179:1188	ANIb <75 %	1179:1188	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between CFH S00084T and the other species of the genus Microbacterium were found to be low (ANIm <85 %, ANIb <75 % and dDDH <24 %).
30575502	10	82	theme	identity	1012:1019	arg1	%					1176:1176	ANIm <85 %	1167:1176	ANIm <85 %	1167:1176	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between CFH S00084T and the other species of the genus Microbacterium were found to be low (ANIm <85 %, ANIb <75 % and dDDH <24 %).
30575502	10	82	theme	identity	1012:1019	arg1	%					1203:1203	dDDH <24 %	1194:1203	dDDH <24 %	1194:1203	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between CFH S00084T and the other species of the genus Microbacterium were found to be low (ANIm <85 %, ANIb <75 % and dDDH <24 %).
30575502	10	82	theme	identity	1012:1019	arg1	low					1162:1164	low	1162:1164	low	1162:1164	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between CFH S00084T and the other species of the genus Microbacterium were found to be low (ANIm <85 %, ANIb <75 % and dDDH <24 %).
30575502	10	82	theme	identity	1012:1019	arg1	values					1068:1073	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values	989:1073	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between CFH S00084T and the other species of the genus Microbacterium	989:1143	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between CFH S00084T and the other species of the genus Microbacterium were found to be low (ANIm <85 %, ANIb <75 % and dDDH <24 %).
30575502	4	83	dep	%	610:610	arg1	w/v					613:615	w/v	613:615	w/v	613:615	The strain grew optimally at 25-37 °C, at pH 7.0 and in 0-3 % (w/v) NaCl.
30575502	10	84	theme	DNA-DNA	1039:1045	arg1	dDDH					1062:1065	dDDH	1062:1065	dDDH	1062:1065	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between CFH S00084T and the other species of the genus Microbacterium were found to be low (ANIm <85 %, ANIb <75 % and dDDH <24 %).
30575502	10	84	theme	DNA-DNA	1039:1045	arg1	hybridization					1047:1059	digital DNA-DNA hybridization	1031:1059	digital DNA-DNA hybridization (dDDH)	1031:1066	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between CFH S00084T and the other species of the genus Microbacterium were found to be low (ANIm <85 %, ANIb <75 % and dDDH <24 %).
30575502	3	85	dep	Microbacterium	422:435	arg1	yannicii					437:444	yannicii	437:444	yannicii	437:444	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CFH S00084T clustered within members of Microbacterium and was most closely related to the type strains Microbacterium yannicii JCM 18959T and Microbacterium arthrosphaerae DSM 22421T (98.97 % and 98.36 % similarity, respectively).
30575502	2	86	theme	non-motile	106:115	arg1	strain					138:143	A Gram-stain positive, aerobic, non-motile and short-rod-shaped strain	74:143	A Gram-stain positive, aerobic, non-motile and short-rod-shaped strain	74:143	A Gram-stain positive, aerobic, non-motile and short-rod-shaped strain, CFH S00084T, was isolated from a sediment sample of the Yellow River in Henan Province, China.
30575502	2	86	theme	non-motile	106:115	arg1	S00084T					150:156	CFH S00084T	146:156	CFH S00084T	146:156	A Gram-stain positive, aerobic, non-motile and short-rod-shaped strain, CFH S00084T, was isolated from a sediment sample of the Yellow River in Henan Province, China.
30575502	10	87	theme	ANIb	1179:1182	arg1	%					1203:1203	dDDH <24 %	1194:1203	dDDH <24 %	1194:1203	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between CFH S00084T and the other species of the genus Microbacterium were found to be low (ANIm <85 %, ANIb <75 % and dDDH <24 %).
30575502	10	87	theme	ANIb	1179:1182	arg1	low					1162:1164	low	1162:1164	low	1162:1164	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between CFH S00084T and the other species of the genus Microbacterium were found to be low (ANIm <85 %, ANIb <75 % and dDDH <24 %).
30575502	10	87	theme	ANIb	1179:1182	arg1	values					1068:1073	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values	989:1073	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between CFH S00084T and the other species of the genus Microbacterium	989:1143	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between CFH S00084T and the other species of the genus Microbacterium were found to be low (ANIm <85 %, ANIb <75 % and dDDH <24 %).
30575502	10	87	theme	ANIb	1179:1182	arg1	%					1176:1176	ANIm <85 %	1167:1176	ANIm <85 %	1167:1176	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between CFH S00084T and the other species of the genus Microbacterium were found to be low (ANIm <85 %, ANIb <75 % and dDDH <24 %).
30575502	10	87	theme	ANIb	1179:1182	arg1	%					1188:1188	ANIb <75 %	1179:1188	ANIb <75 %	1179:1188	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between CFH S00084T and the other species of the genus Microbacterium were found to be low (ANIm <85 %, ANIb <75 % and dDDH <24 %).
29891290	0	0	theme	protein-2	87:95	arg1	delivery					97:104	improved bone morphogenetic protein-2 delivery	59:104	improved bone morphogenetic protein-2 delivery	59:104	Three dimensional cell printing with sulfated alginate for improved bone morphogenetic protein-2 delivery and osteogenesis in bone tissue engineering.
29891290	7	1	theme	3	1352:1352	arg1	%					1353:1353	%	1353:1353	%	1353:1353	Osteoblastic proliferation and differentiation in vitro were promoted by alginate/alginate-sulfate 3D-printed constructs with an optimal composition of 3% alginate and 2% alginate-sulfate.
29891290	2	2	theme	bioink	380:385	arg1	materials					387:395	bioink materials	380:395	bioink materials able to offer good printability and favorable cellular interaction	380:462	To this end, bioink materials able to offer good printability and favorable cellular interaction are highly required.
29891290	1	3	theme	hydrogel	259:266	arg1	scaffolds					268:276	cell-laden hydrogel scaffolds	248:276	cell-laden hydrogel scaffolds with controllable features and interconnected pores	248:328	Three-dimensional (3D) cell printing is a unique technique that enables free-form fabrication of cell-laden hydrogel scaffolds with controllable features and interconnected pores for tissue engineering applications.
29891290	0	4	theme	morphogenetic	73:85	arg1	protein-2					87:95	bone morphogenetic protein-2	68:95	improved bone morphogenetic protein-2 delivery	59:104	Three dimensional cell printing with sulfated alginate for improved bone morphogenetic protein-2 delivery and osteogenesis in bone tissue engineering.
29891290	4	5	from	utilities	821:829	arg1	printing					837:844	3D printing	834:844	3D printing	834:844	Several bio-inks composed of alginate and alginate-sulfate were studied to characterize their material properties and their utilities in 3D printing.
29891290	6	6	theme	3D-printed	1178:1187	arg1	scaffolds					1189:1197	3D-printed scaffolds	1178:1197	3D-printed scaffolds	1178:1197	Moreover, alginate/alginate-sulfate bio-inks exhibited an improved retention of bone morphogenetic protein 2 in 3D-printed scaffolds.
29891290	1	7	theme	scaffolds	268:276	arg1	fabrication					233:243	free-form fabrication	223:243	free-form fabrication of cell-laden hydrogel scaffolds with controllable features and interconnected pores for tissue engineering applications	223:364	Three-dimensional (3D) cell printing is a unique technique that enables free-form fabrication of cell-laden hydrogel scaffolds with controllable features and interconnected pores for tissue engineering applications.
29891290	8	8	theme	bone	1530:1533	arg1	regeneration					1535:1546	bone regeneration	1530:1546	bone regeneration	1530:1546	We envision that bio-inks displaying prolonged interactions with growth factors will be useful for tissue engineering applications including bone regeneration.
29891290	5	9	theme	distinct	1037:1044	arg1	pores					1046:1050	distinct pores	1037:1050	distinct pores	1037:1050	The inclusion of alginate-sulfate in bio-inks (alginate/alginate-sulfate) did not significantly influence their rheological properties and allowed for a good 3D printing processibility with distinct pores and features.
29891290	1	10	theme	controllable	283:294	arg1	features					296:303	controllable features	283:303	controllable features	283:303	Three-dimensional (3D) cell printing is a unique technique that enables free-form fabrication of cell-laden hydrogel scaffolds with controllable features and interconnected pores for tissue engineering applications.
29891290	7	11	theme	alginate-sulfate	1371:1386	arg1	composition					1337:1347	an optimal composition	1326:1347	an optimal composition of 3% alginate and 2% alginate-sulfate	1326:1386	Osteoblastic proliferation and differentiation in vitro were promoted by alginate/alginate-sulfate 3D-printed constructs with an optimal composition of 3% alginate and 2% alginate-sulfate.
29891290	5	12	theme	3D	1005:1006	arg1	processibility					1017:1030	a good 3D printing processibility	998:1030	a good 3D printing processibility with distinct pores and features	998:1063	The inclusion of alginate-sulfate in bio-inks (alginate/alginate-sulfate) did not significantly influence their rheological properties and allowed for a good 3D printing processibility with distinct pores and features.
29891290	2	13	theme	cellular	443:450	arg1	interaction					452:462	favorable cellular interaction	433:462	favorable cellular interaction	433:462	To this end, bioink materials able to offer good printability and favorable cellular interaction are highly required.
29891290	8	14	theme	engineering	1495:1505	arg1	applications					1507:1518	tissue engineering applications	1488:1518	tissue engineering applications including bone regeneration	1488:1546	We envision that bio-inks displaying prolonged interactions with growth factors will be useful for tissue engineering applications including bone regeneration.
29891290	8	14	theme	engineering	1495:1505	arg1	regeneration					1535:1546	bone regeneration	1530:1546	bone regeneration	1530:1546	We envision that bio-inks displaying prolonged interactions with growth factors will be useful for tissue engineering applications including bone regeneration.
29891290	6	15	theme	protein	1165:1171	arg1	retention					1133:1141	an improved retention	1121:1141	an improved retention of bone morphogenetic protein 2 in 3D-printed scaffolds	1121:1197	Moreover, alginate/alginate-sulfate bio-inks exhibited an improved retention of bone morphogenetic protein 2 in 3D-printed scaffolds.
29891290	1	16	theme	cell	174:177	arg1	technique					200:208	a unique technique	191:208	a unique technique that enables free-form fabrication of cell-laden hydrogel scaffolds with controllable features and interconnected pores for tissue engineering applications	191:364	Three-dimensional (3D) cell printing is a unique technique that enables free-form fabrication of cell-laden hydrogel scaffolds with controllable features and interconnected pores for tissue engineering applications.
29891290	1	16	theme	cell	174:177	arg1	printing					179:186	Three-dimensional (3D) cell printing	151:186	Three-dimensional (3D) cell printing	151:186	Three-dimensional (3D) cell printing is a unique technique that enables free-form fabrication of cell-laden hydrogel scaffolds with controllable features and interconnected pores for tissue engineering applications.
29891290	7	17	theme	optimal	1329:1335	arg1	composition					1337:1347	an optimal composition	1326:1347	an optimal composition of 3% alginate and 2% alginate-sulfate	1326:1386	Osteoblastic proliferation and differentiation in vitro were promoted by alginate/alginate-sulfate 3D-printed constructs with an optimal composition of 3% alginate and 2% alginate-sulfate.
29891290	7	18	theme	Osteoblastic	1200:1211	arg1	proliferation					1213:1225	Osteoblastic proliferation	1200:1225	Osteoblastic proliferation	1200:1225	Osteoblastic proliferation and differentiation in vitro were promoted by alginate/alginate-sulfate 3D-printed constructs with an optimal composition of 3% alginate and 2% alginate-sulfate.
29891290	6	19	from	retention	1133:1141	arg1	scaffolds					1189:1197	3D-printed scaffolds	1178:1197	3D-printed scaffolds	1178:1197	Moreover, alginate/alginate-sulfate bio-inks exhibited an improved retention of bone morphogenetic protein 2 in 3D-printed scaffolds.
29891290	5	20	theme	rheological	959:969	arg1	properties					971:980	their rheological properties	953:980	their rheological properties	953:980	The inclusion of alginate-sulfate in bio-inks (alginate/alginate-sulfate) did not significantly influence their rheological properties and allowed for a good 3D printing processibility with distinct pores and features.
29891290	2	21	theme	good	411:414	arg1	printability					416:427	good printability	411:427	good printability	411:427	To this end, bioink materials able to offer good printability and favorable cellular interaction are highly required.
29891290	0	22	theme	dimensional	6:16	arg1	printing					23:30	Three dimensional cell printing	0:30	Three dimensional cell printing with sulfated alginate for improved bone morphogenetic protein-2 delivery and osteogenesis in bone tissue engineering.	0:149	Three dimensional cell printing with sulfated alginate for improved bone morphogenetic protein-2 delivery and osteogenesis in bone tissue engineering.
29891290	5	23	from	inclusion	851:859	arg1	alginate/alginate-sulfate					894:918	alginate/alginate-sulfate	894:918	alginate/alginate-sulfate	894:918	The inclusion of alginate-sulfate in bio-inks (alginate/alginate-sulfate) did not significantly influence their rheological properties and allowed for a good 3D printing processibility with distinct pores and features.
29891290	5	23	from	inclusion	851:859	arg1	bio-inks					884:891	bio-inks	884:891	bio-inks (alginate/alginate-sulfate)	884:919	The inclusion of alginate-sulfate in bio-inks (alginate/alginate-sulfate) did not significantly influence their rheological properties and allowed for a good 3D printing processibility with distinct pores and features.
29891290	7	24	theme	%	1353:1353	arg1	alginate					1355:1362	3% alginate	1352:1362	3% alginate	1352:1362	Osteoblastic proliferation and differentiation in vitro were promoted by alginate/alginate-sulfate 3D-printed constructs with an optimal composition of 3% alginate and 2% alginate-sulfate.
29891290	7	25	theme	alginate	1355:1362	arg1	composition					1337:1347	an optimal composition	1326:1347	an optimal composition of 3% alginate and 2% alginate-sulfate	1326:1386	Osteoblastic proliferation and differentiation in vitro were promoted by alginate/alginate-sulfate 3D-printed constructs with an optimal composition of 3% alginate and 2% alginate-sulfate.
29891290	5	26	theme	good	1000:1003	arg1	processibility					1017:1030	a good 3D printing processibility	998:1030	a good 3D printing processibility with distinct pores and features	998:1063	The inclusion of alginate-sulfate in bio-inks (alginate/alginate-sulfate) did not significantly influence their rheological properties and allowed for a good 3D printing processibility with distinct pores and features.
29891290	4	27	theme	Several	697:703	arg1	bio-inks					705:712	Several bio-inks	697:712	Several bio-inks composed of alginate and alginate-sulfate	697:754	Several bio-inks composed of alginate and alginate-sulfate were studied to characterize their material properties and their utilities in 3D printing.
29891290	5	28	with	processibility	1017:1030	arg1	features					1056:1063	features	1056:1063	features	1056:1063	The inclusion of alginate-sulfate in bio-inks (alginate/alginate-sulfate) did not significantly influence their rheological properties and allowed for a good 3D printing processibility with distinct pores and features.
29891290	5	28	with	processibility	1017:1030	arg1	pores					1046:1050	distinct pores	1037:1050	distinct pores	1037:1050	The inclusion of alginate-sulfate in bio-inks (alginate/alginate-sulfate) did not significantly influence their rheological properties and allowed for a good 3D printing processibility with distinct pores and features.
29891290	3	29	theme	growth	593:598	arg1	factors					600:606	growth factors	593:606	growth factors	593:606	Herein, we synthesized alginate sulfate, which is a structural mimic of heparin that can strongly bind with growth factors to prolong their activities, and studied its feasibility for cell printing applications.
29891290	6	30	theme	morphogenetic	1151:1163	arg1	protein					1165:1171	bone morphogenetic protein 2	1146:1173	bone morphogenetic protein 2	1146:1173	Moreover, alginate/alginate-sulfate bio-inks exhibited an improved retention of bone morphogenetic protein 2 in 3D-printed scaffolds.
29891290	7	31	theme	alginate/alginate-sulfate	1273:1297	arg1	constructs					1310:1319	alginate/alginate-sulfate 3D-printed constructs	1273:1319	alginate/alginate-sulfate 3D-printed constructs with an optimal composition of 3% alginate and 2% alginate-sulfate	1273:1386	Osteoblastic proliferation and differentiation in vitro were promoted by alginate/alginate-sulfate 3D-printed constructs with an optimal composition of 3% alginate and 2% alginate-sulfate.
29891290	0	32	theme	tissue	131:136	arg1	engineering					138:148	bone tissue engineering	126:148	bone tissue engineering	126:148	Three dimensional cell printing with sulfated alginate for improved bone morphogenetic protein-2 delivery and osteogenesis in bone tissue engineering.
29891290	4	33	from	properties	800:809	arg1	printing					837:844	3D printing	834:844	3D printing	834:844	Several bio-inks composed of alginate and alginate-sulfate were studied to characterize their material properties and their utilities in 3D printing.
29891290	1	34	dep	Three-dimensional	151:167	arg1	3D					170:171	3D	170:171	3D	170:171	Three-dimensional (3D) cell printing is a unique technique that enables free-form fabrication of cell-laden hydrogel scaffolds with controllable features and interconnected pores for tissue engineering applications.
29891290	1	35	theme	unique	193:198	arg1	technique					200:208	a unique technique	191:208	a unique technique that enables free-form fabrication of cell-laden hydrogel scaffolds with controllable features and interconnected pores for tissue engineering applications	191:364	Three-dimensional (3D) cell printing is a unique technique that enables free-form fabrication of cell-laden hydrogel scaffolds with controllable features and interconnected pores for tissue engineering applications.
29891290	1	35	theme	unique	193:198	arg1	printing					179:186	Three-dimensional (3D) cell printing	151:186	Three-dimensional (3D) cell printing	151:186	Three-dimensional (3D) cell printing is a unique technique that enables free-form fabrication of cell-laden hydrogel scaffolds with controllable features and interconnected pores for tissue engineering applications.
29891290	0	36	theme	cell	18:21	arg1	printing					23:30	Three dimensional cell printing	0:30	Three dimensional cell printing with sulfated alginate for improved bone morphogenetic protein-2 delivery and osteogenesis in bone tissue engineering.	0:149	Three dimensional cell printing with sulfated alginate for improved bone morphogenetic protein-2 delivery and osteogenesis in bone tissue engineering.
29891290	1	37	theme	interconnected	309:322	arg1	pores					324:328	interconnected pores	309:328	interconnected pores	309:328	Three-dimensional (3D) cell printing is a unique technique that enables free-form fabrication of cell-laden hydrogel scaffolds with controllable features and interconnected pores for tissue engineering applications.
29891290	0	38	theme	bone	126:129	arg1	engineering					138:148	bone tissue engineering	126:148	bone tissue engineering	126:148	Three dimensional cell printing with sulfated alginate for improved bone morphogenetic protein-2 delivery and osteogenesis in bone tissue engineering.
29891290	7	39	theme	%	1369:1369	arg1	alginate-sulfate					1371:1386	2% alginate-sulfate	1368:1386	2% alginate-sulfate	1368:1386	Osteoblastic proliferation and differentiation in vitro were promoted by alginate/alginate-sulfate 3D-printed constructs with an optimal composition of 3% alginate and 2% alginate-sulfate.
29891290	2	40	theme	able	397:400	arg1	materials					387:395	bioink materials	380:395	bioink materials able to offer good printability and favorable cellular interaction	380:462	To this end, bioink materials able to offer good printability and favorable cellular interaction are highly required.
29891290	0	41	theme	sulfated	37:44	arg1	alginate					46:53	sulfated alginate	37:53	sulfated alginate for improved bone morphogenetic protein-2 delivery and osteogenesis	37:121	Three dimensional cell printing with sulfated alginate for improved bone morphogenetic protein-2 delivery and osteogenesis in bone tissue engineering.
29891290	3	42	theme	cell	669:672	arg1	applications					683:694	cell printing applications	669:694	cell printing applications	669:694	Herein, we synthesized alginate sulfate, which is a structural mimic of heparin that can strongly bind with growth factors to prolong their activities, and studied its feasibility for cell printing applications.
29891290	2	43	theme	favorable	433:441	arg1	interaction					452:462	favorable cellular interaction	433:462	favorable cellular interaction	433:462	To this end, bioink materials able to offer good printability and favorable cellular interaction are highly required.
29891290	6	44	theme	alginate/alginate-sulfate	1076:1100	arg1	bio-inks					1102:1109	alginate/alginate-sulfate bio-inks	1076:1109	alginate/alginate-sulfate bio-inks	1076:1109	Moreover, alginate/alginate-sulfate bio-inks exhibited an improved retention of bone morphogenetic protein 2 in 3D-printed scaffolds.
29891290	0	45	with	printing	23:30	arg1	alginate					46:53	sulfated alginate	37:53	sulfated alginate for improved bone morphogenetic protein-2 delivery and osteogenesis	37:121	Three dimensional cell printing with sulfated alginate for improved bone morphogenetic protein-2 delivery and osteogenesis in bone tissue engineering.
29891290	6	46	theme	improved	1124:1131	arg1	retention					1133:1141	an improved retention	1121:1141	an improved retention of bone morphogenetic protein 2 in 3D-printed scaffolds	1121:1197	Moreover, alginate/alginate-sulfate bio-inks exhibited an improved retention of bone morphogenetic protein 2 in 3D-printed scaffolds.
29891290	1	47	theme	tissue	334:339	arg1	applications					353:364	tissue engineering applications	334:364	tissue engineering applications	334:364	Three-dimensional (3D) cell printing is a unique technique that enables free-form fabrication of cell-laden hydrogel scaffolds with controllable features and interconnected pores for tissue engineering applications.
29891290	8	48	theme	prolonged	1426:1434	arg1	interactions					1436:1447	prolonged interactions	1426:1447	prolonged interactions with growth factors	1426:1467	We envision that bio-inks displaying prolonged interactions with growth factors will be useful for tissue engineering applications including bone regeneration.
29891290	5	49	theme	printing	1008:1015	arg1	processibility					1017:1030	a good 3D printing processibility	998:1030	a good 3D printing processibility with distinct pores and features	998:1063	The inclusion of alginate-sulfate in bio-inks (alginate/alginate-sulfate) did not significantly influence their rheological properties and allowed for a good 3D printing processibility with distinct pores and features.
29891290	1	50	theme	engineering	341:351	arg1	applications					353:364	tissue engineering applications	334:364	tissue engineering applications	334:364	Three-dimensional (3D) cell printing is a unique technique that enables free-form fabrication of cell-laden hydrogel scaffolds with controllable features and interconnected pores for tissue engineering applications.
29891290	7	51	with	constructs	1310:1319	arg1	composition					1337:1347	an optimal composition	1326:1347	an optimal composition of 3% alginate and 2% alginate-sulfate	1326:1386	Osteoblastic proliferation and differentiation in vitro were promoted by alginate/alginate-sulfate 3D-printed constructs with an optimal composition of 3% alginate and 2% alginate-sulfate.
29891290	4	52	theme	3D	834:835	arg1	printing					837:844	3D printing	834:844	3D printing	834:844	Several bio-inks composed of alginate and alginate-sulfate were studied to characterize their material properties and their utilities in 3D printing.
29891290	1	53	with	scaffolds	268:276	arg1	features					296:303	controllable features	283:303	controllable features	283:303	Three-dimensional (3D) cell printing is a unique technique that enables free-form fabrication of cell-laden hydrogel scaffolds with controllable features and interconnected pores for tissue engineering applications.
29891290	1	53	with	scaffolds	268:276	arg1	pores					324:328	interconnected pores	309:328	interconnected pores	309:328	Three-dimensional (3D) cell printing is a unique technique that enables free-form fabrication of cell-laden hydrogel scaffolds with controllable features and interconnected pores for tissue engineering applications.
29891290	3	54	theme	alginate	508:515	arg1	sulfate					517:523	alginate sulfate	508:523	alginate sulfate	508:523	Herein, we synthesized alginate sulfate, which is a structural mimic of heparin that can strongly bind with growth factors to prolong their activities, and studied its feasibility for cell printing applications.
29891290	3	54	theme	alginate	508:515	arg1	mimic					548:552	mimic	548:552	mimic	548:552	Herein, we synthesized alginate sulfate, which is a structural mimic of heparin that can strongly bind with growth factors to prolong their activities, and studied its feasibility for cell printing applications.
29891290	4	55	theme	material	791:798	arg1	properties					800:809	their material properties	785:809	their material properties	785:809	Several bio-inks composed of alginate and alginate-sulfate were studied to characterize their material properties and their utilities in 3D printing.
29891290	1	56	theme	free-form	223:231	arg1	fabrication					233:243	free-form fabrication	223:243	free-form fabrication of cell-laden hydrogel scaffolds with controllable features and interconnected pores for tissue engineering applications	223:364	Three-dimensional (3D) cell printing is a unique technique that enables free-form fabrication of cell-laden hydrogel scaffolds with controllable features and interconnected pores for tissue engineering applications.
29891290	8	57	theme	tissue	1488:1493	arg1	applications					1507:1518	tissue engineering applications	1488:1518	tissue engineering applications including bone regeneration	1488:1546	We envision that bio-inks displaying prolonged interactions with growth factors will be useful for tissue engineering applications including bone regeneration.
29891290	8	57	theme	tissue	1488:1493	arg1	regeneration					1535:1546	bone regeneration	1530:1546	bone regeneration	1530:1546	We envision that bio-inks displaying prolonged interactions with growth factors will be useful for tissue engineering applications including bone regeneration.
29891290	3	58	theme	printing	674:681	arg1	applications					683:694	cell printing applications	669:694	cell printing applications	669:694	Herein, we synthesized alginate sulfate, which is a structural mimic of heparin that can strongly bind with growth factors to prolong their activities, and studied its feasibility for cell printing applications.
29891290	8	59	theme	growth	1454:1459	arg1	factors					1461:1467	growth factors	1454:1467	growth factors	1454:1467	We envision that bio-inks displaying prolonged interactions with growth factors will be useful for tissue engineering applications including bone regeneration.
29891290	8	60	with	interactions	1436:1447	arg1	factors					1461:1467	growth factors	1454:1467	growth factors	1454:1467	We envision that bio-inks displaying prolonged interactions with growth factors will be useful for tissue engineering applications including bone regeneration.
29891290	0	61	theme	bone	68:71	arg1	protein-2					87:95	bone morphogenetic protein-2	68:95	improved bone morphogenetic protein-2 delivery	59:104	Three dimensional cell printing with sulfated alginate for improved bone morphogenetic protein-2 delivery and osteogenesis in bone tissue engineering.
29891290	6	62	theme	bone	1146:1149	arg1	protein					1165:1171	bone morphogenetic protein 2	1146:1173	bone morphogenetic protein 2	1146:1173	Moreover, alginate/alginate-sulfate bio-inks exhibited an improved retention of bone morphogenetic protein 2 in 3D-printed scaffolds.
29891290	1	63	theme	Three-dimensional	151:167	arg1	technique					200:208	a unique technique	191:208	a unique technique that enables free-form fabrication of cell-laden hydrogel scaffolds with controllable features and interconnected pores for tissue engineering applications	191:364	Three-dimensional (3D) cell printing is a unique technique that enables free-form fabrication of cell-laden hydrogel scaffolds with controllable features and interconnected pores for tissue engineering applications.
29891290	1	63	theme	Three-dimensional	151:167	arg1	printing					179:186	Three-dimensional (3D) cell printing	151:186	Three-dimensional (3D) cell printing	151:186	Three-dimensional (3D) cell printing is a unique technique that enables free-form fabrication of cell-laden hydrogel scaffolds with controllable features and interconnected pores for tissue engineering applications.
29891290	0	64	theme	improved	59:66	arg1	delivery					97:104	improved bone morphogenetic protein-2 delivery	59:104	improved bone morphogenetic protein-2 delivery	59:104	Three dimensional cell printing with sulfated alginate for improved bone morphogenetic protein-2 delivery and osteogenesis in bone tissue engineering.
29891290	0	65	from	printing	23:30	arg1	engineering					138:148	bone tissue engineering	126:148	bone tissue engineering	126:148	Three dimensional cell printing with sulfated alginate for improved bone morphogenetic protein-2 delivery and osteogenesis in bone tissue engineering.
29891290	5	66	theme	alginate-sulfate	864:879	arg1	inclusion					851:859	The inclusion	847:859	The inclusion of alginate-sulfate in bio-inks (alginate/alginate-sulfate)	847:919	The inclusion of alginate-sulfate in bio-inks (alginate/alginate-sulfate) did not significantly influence their rheological properties and allowed for a good 3D printing processibility with distinct pores and features.
29891290	7	67	theme	3D-printed	1299:1308	arg1	constructs					1310:1319	alginate/alginate-sulfate 3D-printed constructs	1273:1319	alginate/alginate-sulfate 3D-printed constructs with an optimal composition of 3% alginate and 2% alginate-sulfate	1273:1386	Osteoblastic proliferation and differentiation in vitro were promoted by alginate/alginate-sulfate 3D-printed constructs with an optimal composition of 3% alginate and 2% alginate-sulfate.
29891290	7	68	theme	2	1368:1368	arg1	%					1369:1369	%	1369:1369	%	1369:1369	Osteoblastic proliferation and differentiation in vitro were promoted by alginate/alginate-sulfate 3D-printed constructs with an optimal composition of 3% alginate and 2% alginate-sulfate.
29891290	1	69	theme	cell-laden	248:257	arg1	scaffolds					268:276	cell-laden hydrogel scaffolds	248:276	cell-laden hydrogel scaffolds with controllable features and interconnected pores	248:328	Three-dimensional (3D) cell printing is a unique technique that enables free-form fabrication of cell-laden hydrogel scaffolds with controllable features and interconnected pores for tissue engineering applications.
30088952	7	0	from	DFUs	1066:1069	arg1	fibroblasts					1049:1059	iPSC-derived fibroblasts	1036:1059	iPSC-derived fibroblasts from DFUs	1036:1069	iPSC-derived fibroblasts from DFUs displayed a unique biochemical composition and morphology when grown as 3-dimensional (3D), self-assembled extracellular matrix tissues, which were distinct from tissues fabricated using the parental DFU fibroblasts from which they were reprogrammed.
30088952	7	1	theme	3-dimensional	1143:1155	arg1	tissues					1199:1205	3-dimensional (3D), self-assembled extracellular matrix tissues	1143:1205	tissues	1199:1205	iPSC-derived fibroblasts from DFUs displayed a unique biochemical composition and morphology when grown as 3-dimensional (3D), self-assembled extracellular matrix tissues, which were distinct from tissues fabricated using the parental DFU fibroblasts from which they were reprogrammed.
30088952	1	2	theme	novel	233:237	arg1	therapies					262:270	novel cell- and tissue-based therapies	233:270	novel cell- and tissue-based therapies	233:270	Diabetic foot ulcers (DFUs) are a major complication of diabetes, and there is a critical need to develop novel cell- and tissue-based therapies to treat these chronic wounds.
30088952	9	3	dep	A.	1821:1822	arg1	M.					1782:1783	M.	1782:1783	M.	1782:1783	Taken together, our findings support the potential application of these iPSC-derived fibroblasts and 3D tissues to improve wound healing.-Kashpur, O., Smith, A., Gerami-Naini, B., Maione, A. G., Calabrese, R., Tellechea, A., Theocharidis, G., Liang, L., Pastar, I., Tomic-Canic, M., Mooney, D., Veves, A., Garlick, J. A. Differentiation of diabetic foot ulcer-derived induced pluripotent stem cells reveals distinct cellular and tissue phenotypes.
30088952	8	4	theme	tissues	1352:1358	arg1	transplantation					1330:1344	In vivo transplantation	1322:1344	In vivo transplantation of 3D tissues with iPSC-derived fibroblasts	1322:1388	In vivo transplantation of 3D tissues with iPSC-derived fibroblasts showed they persisted in the wound and facilitated diabetic wound closure compared with primary DFU fibroblasts.
30088952	3	5	theme	iPSC-derived	507:518	arg1	cells					520:524	iPSC-derived cells	507:524	iPSC-derived cells	507:524	However, the biologic potential of iPSC-derived cells to treat DFUs has not, to our knowledge, been investigated.
30088952	4	6	theme	detailed	622:629	arg1	characterization					631:646	detailed characterization	622:646	detailed characterization of iPSC-derived fibroblasts from both diabetic and nondiabetic patients	622:718	Toward that goal, we have performed detailed characterization of iPSC-derived fibroblasts from both diabetic and nondiabetic patients.
30088952	1	7	theme	diabetes	183:190	arg1	ulcers					141:146	Diabetic foot ulcers	127:146	Diabetic foot ulcers (DFUs)	127:153	Diabetic foot ulcers (DFUs) are a major complication of diabetes, and there is a critical need to develop novel cell- and tissue-based therapies to treat these chronic wounds.
30088952	1	7	theme	diabetes	183:190	arg1	complication					167:178	a major complication	159:178	a major complication of diabetes	159:190	Diabetic foot ulcers (DFUs) are a major complication of diabetes, and there is a critical need to develop novel cell- and tissue-based therapies to treat these chronic wounds.
30088952	0	8	link	ulcer-derived	33:45	arg1	cells					72:76	diabetic foot ulcer-derived induced pluripotent stem cells	19:76	diabetic foot ulcer-derived induced pluripotent stem cells	19:76	Differentiation of diabetic foot ulcer-derived induced pluripotent stem cells reveals distinct cellular and tissue phenotypes.
30088952	7	9	dep	3-dimensional	1143:1155	arg1	3D					1158:1159	3D	1158:1159	3D	1158:1159	iPSC-derived fibroblasts from DFUs displayed a unique biochemical composition and morphology when grown as 3-dimensional (3D), self-assembled extracellular matrix tissues, which were distinct from tissues fabricated using the parental DFU fibroblasts from which they were reprogrammed.
30088952	7	10	theme	unique	1083:1088	arg1	composition					1102:1112	a unique biochemical composition	1081:1112	a unique biochemical composition	1081:1112	iPSC-derived fibroblasts from DFUs displayed a unique biochemical composition and morphology when grown as 3-dimensional (3D), self-assembled extracellular matrix tissues, which were distinct from tissues fabricated using the parental DFU fibroblasts from which they were reprogrammed.
30088952	8	11	theme	primary	1478:1484	arg1	fibroblasts					1490:1500	primary DFU fibroblasts	1478:1500	primary DFU fibroblasts	1478:1500	In vivo transplantation of 3D tissues with iPSC-derived fibroblasts showed they persisted in the wound and facilitated diabetic wound closure compared with primary DFU fibroblasts.
30088952	7	12	theme	self-assembled	1163:1176	arg1	tissues					1199:1205	3-dimensional (3D), self-assembled extracellular matrix tissues	1143:1205	tissues	1199:1205	iPSC-derived fibroblasts from DFUs displayed a unique biochemical composition and morphology when grown as 3-dimensional (3D), self-assembled extracellular matrix tissues, which were distinct from tissues fabricated using the parental DFU fibroblasts from which they were reprogrammed.
30088952	8	13	theme	In	1322:1323	arg1	transplantation					1330:1344	In vivo transplantation	1322:1344	In vivo transplantation of 3D tissues with iPSC-derived fibroblasts	1322:1388	In vivo transplantation of 3D tissues with iPSC-derived fibroblasts showed they persisted in the wound and facilitated diabetic wound closure compared with primary DFU fibroblasts.
30088952	9	14	theme	induced	1871:1877	arg1	cells					1896:1900	induced pluripotent stem cells	1871:1900	induced pluripotent stem cells	1871:1900	Taken together, our findings support the potential application of these iPSC-derived fibroblasts and 3D tissues to improve wound healing.-Kashpur, O., Smith, A., Gerami-Naini, B., Maione, A. G., Calabrese, R., Tellechea, A., Theocharidis, G., Liang, L., Pastar, I., Tomic-Canic, M., Mooney, D., Veves, A., Garlick, J. A. Differentiation of diabetic foot ulcer-derived induced pluripotent stem cells reveals distinct cellular and tissue phenotypes.
30088952	5	15	from	patients	818:825	arg1	fibroblasts					796:806	iPSC-derived fibroblasts	783:806	iPSC-derived fibroblasts from both patients with and those without diabetes	783:857	Significantly, gene array and functional analyses reveal that iPSC-derived fibroblasts from both patients with and those without diabetes are more similar to each other than were the primary cells from which they were derived.
30088952	5	15	from	patients	818:825	arg1	cells					912:916	the primary cells	900:916	the primary cells from which they were derived	900:945	Significantly, gene array and functional analyses reveal that iPSC-derived fibroblasts from both patients with and those without diabetes are more similar to each other than were the primary cells from which they were derived.
30088952	1	16	theme	foot	136:139	arg1	ulcers					141:146	Diabetic foot ulcers	127:146	Diabetic foot ulcers (DFUs)	127:153	Diabetic foot ulcers (DFUs) are a major complication of diabetes, and there is a critical need to develop novel cell- and tissue-based therapies to treat these chronic wounds.
30088952	1	16	theme	foot	136:139	arg1	complication					167:178	a major complication	159:178	a major complication of diabetes	159:190	Diabetic foot ulcers (DFUs) are a major complication of diabetes, and there is a critical need to develop novel cell- and tissue-based therapies to treat these chronic wounds.
30088952	1	16	theme	foot	136:139	arg1	DFUs					149:152	DFUs	149:152	DFUs	149:152	Diabetic foot ulcers (DFUs) are a major complication of diabetes, and there is a critical need to develop novel cell- and tissue-based therapies to treat these chronic wounds.
30088952	7	17	from	tissues	1233:1239	arg1	distinct					1219:1226	distinct	1219:1226	distinct	1219:1226	iPSC-derived fibroblasts from DFUs displayed a unique biochemical composition and morphology when grown as 3-dimensional (3D), self-assembled extracellular matrix tissues, which were distinct from tissues fabricated using the parental DFU fibroblasts from which they were reprogrammed.
30088952	9	18	theme	stem	1891:1894	arg1	cells					1896:1900	induced pluripotent stem cells	1871:1900	induced pluripotent stem cells	1871:1900	Taken together, our findings support the potential application of these iPSC-derived fibroblasts and 3D tissues to improve wound healing.-Kashpur, O., Smith, A., Gerami-Naini, B., Maione, A. G., Calabrese, R., Tellechea, A., Theocharidis, G., Liang, L., Pastar, I., Tomic-Canic, M., Mooney, D., Veves, A., Garlick, J. A. Differentiation of diabetic foot ulcer-derived induced pluripotent stem cells reveals distinct cellular and tissue phenotypes.
30088952	9	19	dep	application	1554:1564	arg1	reveals					1902:1908	reveals	1902:1908	reveals distinct cellular	1902:1926	Taken together, our findings support the potential application of these iPSC-derived fibroblasts and 3D tissues to improve wound healing.-Kashpur, O., Smith, A., Gerami-Naini, B., Maione, A. G., Calabrese, R., Tellechea, A., Theocharidis, G., Liang, L., Pastar, I., Tomic-Canic, M., Mooney, D., Veves, A., Garlick, J. A. Differentiation of diabetic foot ulcer-derived induced pluripotent stem cells reveals distinct cellular and tissue phenotypes.
30088952	7	20	theme	iPSC-derived	1036:1047	arg1	fibroblasts					1049:1059	iPSC-derived fibroblasts	1036:1059	iPSC-derived fibroblasts from DFUs	1036:1069	iPSC-derived fibroblasts from DFUs displayed a unique biochemical composition and morphology when grown as 3-dimensional (3D), self-assembled extracellular matrix tissues, which were distinct from tissues fabricated using the parental DFU fibroblasts from which they were reprogrammed.
30088952	9	21	theme	potential	1544:1552	arg1	application					1554:1564	the potential application	1540:1564	the potential application of these iPSC-derived fibroblasts and 3D tissues to improve wound healing.-Kashpur, O., Smith, A., Gerami-Naini, B., Maione, A. G., Calabrese, R., Tellechea, A., Theocharidis, G., Liang, L., Pastar, I., Tomic-Canic, M., Mooney, D., Veves, A., Garlick, J. A. Differentiation of diabetic foot ulcer-derived induced pluripotent stem cells reveals distinct cellular	1540:1926	Taken together, our findings support the potential application of these iPSC-derived fibroblasts and 3D tissues to improve wound healing.-Kashpur, O., Smith, A., Gerami-Naini, B., Maione, A. G., Calabrese, R., Tellechea, A., Theocharidis, G., Liang, L., Pastar, I., Tomic-Canic, M., Mooney, D., Veves, A., Garlick, J. A. Differentiation of diabetic foot ulcer-derived induced pluripotent stem cells reveals distinct cellular and tissue phenotypes.
30088952	6	22	theme	2-dimensional	1013:1025	arg1	culture					1027:1033	2-dimensional culture	1013:1033	2-dimensional culture	1013:1033	iPSC-derived fibroblasts showed improved migratory properties in 2-dimensional culture.
30088952	5	23	theme	gene	736:739	arg1	array					741:745	gene array	736:745	gene array	736:745	Significantly, gene array and functional analyses reveal that iPSC-derived fibroblasts from both patients with and those without diabetes are more similar to each other than were the primary cells from which they were derived.
30088952	0	24	theme	diabetic	19:26	arg1	cells					72:76	diabetic foot ulcer-derived induced pluripotent stem cells	19:76	diabetic foot ulcer-derived induced pluripotent stem cells	19:76	Differentiation of diabetic foot ulcer-derived induced pluripotent stem cells reveals distinct cellular and tissue phenotypes.
30088952	4	25	theme	fibroblasts	664:674	arg1	characterization					631:646	detailed characterization	622:646	detailed characterization of iPSC-derived fibroblasts from both diabetic and nondiabetic patients	622:718	Toward that goal, we have performed detailed characterization of iPSC-derived fibroblasts from both diabetic and nondiabetic patients.
30088952	9	26	theme	iPSC-derived	1575:1586	arg1	fibroblasts					1588:1598	these iPSC-derived fibroblasts	1569:1598	these iPSC-derived fibroblasts	1569:1598	Taken together, our findings support the potential application of these iPSC-derived fibroblasts and 3D tissues to improve wound healing.-Kashpur, O., Smith, A., Gerami-Naini, B., Maione, A. G., Calabrese, R., Tellechea, A., Theocharidis, G., Liang, L., Pastar, I., Tomic-Canic, M., Mooney, D., Veves, A., Garlick, J. A. Differentiation of diabetic foot ulcer-derived induced pluripotent stem cells reveals distinct cellular and tissue phenotypes.
30088952	6	27	theme	improved	980:987	arg1	properties					999:1008	improved migratory properties	980:1008	improved migratory properties	980:1008	iPSC-derived fibroblasts showed improved migratory properties in 2-dimensional culture.
30088952	9	28	theme	tissue	1932:1937	arg1	phenotypes					1939:1948	tissue phenotypes	1932:1948	tissue phenotypes	1932:1948	Taken together, our findings support the potential application of these iPSC-derived fibroblasts and 3D tissues to improve wound healing.-Kashpur, O., Smith, A., Gerami-Naini, B., Maione, A. G., Calabrese, R., Tellechea, A., Theocharidis, G., Liang, L., Pastar, I., Tomic-Canic, M., Mooney, D., Veves, A., Garlick, J. A. Differentiation of diabetic foot ulcer-derived induced pluripotent stem cells reveals distinct cellular and tissue phenotypes.
30088952	8	29	theme	iPSC-derived	1365:1376	arg1	fibroblasts					1378:1388	iPSC-derived fibroblasts	1365:1388	iPSC-derived fibroblasts	1365:1388	In vivo transplantation of 3D tissues with iPSC-derived fibroblasts showed they persisted in the wound and facilitated diabetic wound closure compared with primary DFU fibroblasts.
30088952	5	30	theme	primary	904:910	arg1	fibroblasts					796:806	iPSC-derived fibroblasts	783:806	iPSC-derived fibroblasts from both patients with and those without diabetes	783:857	Significantly, gene array and functional analyses reveal that iPSC-derived fibroblasts from both patients with and those without diabetes are more similar to each other than were the primary cells from which they were derived.
30088952	5	30	theme	primary	904:910	arg1	cells					912:916	the primary cells	900:916	the primary cells from which they were derived	900:945	Significantly, gene array and functional analyses reveal that iPSC-derived fibroblasts from both patients with and those without diabetes are more similar to each other than were the primary cells from which they were derived.
30088952	0	31	theme	induced	47:53	arg1	cells					72:76	diabetic foot ulcer-derived induced pluripotent stem cells	19:76	diabetic foot ulcer-derived induced pluripotent stem cells	19:76	Differentiation of diabetic foot ulcer-derived induced pluripotent stem cells reveals distinct cellular and tissue phenotypes.
30088952	2	32	theme	replenishing	350:361	arg1	source					363:368	a replenishing source	348:368	a replenishing source of allogeneic and autologous cell types that may be beneficial to improve DFU wound-healing outcomes	348:469	Induced pluripotent stem cells (iPSCs) offer a replenishing source of allogeneic and autologous cell types that may be beneficial to improve DFU wound-healing outcomes.
30088952	2	32	theme	replenishing	350:361	arg1	beneficial					422:431	beneficial	422:431	beneficial	422:431	Induced pluripotent stem cells (iPSCs) offer a replenishing source of allogeneic and autologous cell types that may be beneficial to improve DFU wound-healing outcomes.
30088952	1	33	theme	tissue-based	249:260	arg1	therapies					262:270	novel cell- and tissue-based therapies	233:270	novel cell- and tissue-based therapies	233:270	Diabetic foot ulcers (DFUs) are a major complication of diabetes, and there is a critical need to develop novel cell- and tissue-based therapies to treat these chronic wounds.
30088952	4	34	from	patients	711:718	arg1	characterization					631:646	detailed characterization	622:646	detailed characterization of iPSC-derived fibroblasts from both diabetic and nondiabetic patients	622:718	Toward that goal, we have performed detailed characterization of iPSC-derived fibroblasts from both diabetic and nondiabetic patients.
30088952	4	34	from	patients	711:718	arg1	fibroblasts					664:674	iPSC-derived fibroblasts	651:674	iPSC-derived fibroblasts from both diabetic and nondiabetic patients	651:718	Toward that goal, we have performed detailed characterization of iPSC-derived fibroblasts from both diabetic and nondiabetic patients.
30088952	5	35	dep	similar	868:874	arg1	fibroblasts					796:806	iPSC-derived fibroblasts	783:806	iPSC-derived fibroblasts from both patients with and those without diabetes	783:857	Significantly, gene array and functional analyses reveal that iPSC-derived fibroblasts from both patients with and those without diabetes are more similar to each other than were the primary cells from which they were derived.
30088952	5	35	dep	similar	868:874	arg1	cells					912:916	the primary cells	900:916	the primary cells from which they were derived	900:945	Significantly, gene array and functional analyses reveal that iPSC-derived fibroblasts from both patients with and those without diabetes are more similar to each other than were the primary cells from which they were derived.
30088952	2	36	theme	cell	399:402	arg1	types					404:408	allogeneic and autologous cell types	373:408	allogeneic and autologous cell types	373:408	Induced pluripotent stem cells (iPSCs) offer a replenishing source of allogeneic and autologous cell types that may be beneficial to improve DFU wound-healing outcomes.
30088952	7	37	theme	extracellular	1178:1190	arg1	tissues					1199:1205	3-dimensional (3D), self-assembled extracellular matrix tissues	1143:1205	tissues	1199:1205	iPSC-derived fibroblasts from DFUs displayed a unique biochemical composition and morphology when grown as 3-dimensional (3D), self-assembled extracellular matrix tissues, which were distinct from tissues fabricated using the parental DFU fibroblasts from which they were reprogrammed.
30088952	9	38	link	iPSC-derived	1575:1586	arg1	fibroblasts					1588:1598	these iPSC-derived fibroblasts	1569:1598	these iPSC-derived fibroblasts	1569:1598	Taken together, our findings support the potential application of these iPSC-derived fibroblasts and 3D tissues to improve wound healing.-Kashpur, O., Smith, A., Gerami-Naini, B., Maione, A. G., Calabrese, R., Tellechea, A., Theocharidis, G., Liang, L., Pastar, I., Tomic-Canic, M., Mooney, D., Veves, A., Garlick, J. A. Differentiation of diabetic foot ulcer-derived induced pluripotent stem cells reveals distinct cellular and tissue phenotypes.
30088952	2	39	theme	DFU	444:446	arg1	outcomes					462:469	DFU wound-healing outcomes	444:469	DFU wound-healing outcomes	444:469	Induced pluripotent stem cells (iPSCs) offer a replenishing source of allogeneic and autologous cell types that may be beneficial to improve DFU wound-healing outcomes.
30088952	0	40	theme	ulcer-derived	33:45	arg1	cells					72:76	diabetic foot ulcer-derived induced pluripotent stem cells	19:76	diabetic foot ulcer-derived induced pluripotent stem cells	19:76	Differentiation of diabetic foot ulcer-derived induced pluripotent stem cells reveals distinct cellular and tissue phenotypes.
30088952	0	41	theme	cells	72:76	arg1	Differentiation					0:14	Differentiation	0:14	Differentiation of diabetic foot ulcer-derived induced pluripotent stem cells	0:76	Differentiation of diabetic foot ulcer-derived induced pluripotent stem cells reveals distinct cellular and tissue phenotypes.
30088952	8	42	theme	diabetic	1441:1448	arg1	closure					1456:1462	diabetic wound closure	1441:1462	diabetic wound closure	1441:1462	In vivo transplantation of 3D tissues with iPSC-derived fibroblasts showed they persisted in the wound and facilitated diabetic wound closure compared with primary DFU fibroblasts.
30088952	0	43	theme	pluripotent	55:65	arg1	cells					72:76	diabetic foot ulcer-derived induced pluripotent stem cells	19:76	diabetic foot ulcer-derived induced pluripotent stem cells	19:76	Differentiation of diabetic foot ulcer-derived induced pluripotent stem cells reveals distinct cellular and tissue phenotypes.
30088952	2	44	theme	pluripotent	311:321	arg1	iPSCs					335:339	iPSCs	335:339	iPSCs	335:339	Induced pluripotent stem cells (iPSCs) offer a replenishing source of allogeneic and autologous cell types that may be beneficial to improve DFU wound-healing outcomes.
30088952	2	44	theme	pluripotent	311:321	arg1	cells					328:332	Induced pluripotent stem cells	303:332	Induced pluripotent stem cells (iPSCs)	303:340	Induced pluripotent stem cells (iPSCs) offer a replenishing source of allogeneic and autologous cell types that may be beneficial to improve DFU wound-healing outcomes.
30088952	8	45	dep	In	1322:1323	arg1	vivo					1325:1328	vivo	1325:1328	vivo	1325:1328	In vivo transplantation of 3D tissues with iPSC-derived fibroblasts showed they persisted in the wound and facilitated diabetic wound closure compared with primary DFU fibroblasts.
30088952	8	46	with	transplantation	1330:1344	arg1	fibroblasts					1378:1388	iPSC-derived fibroblasts	1365:1388	iPSC-derived fibroblasts	1365:1388	In vivo transplantation of 3D tissues with iPSC-derived fibroblasts showed they persisted in the wound and facilitated diabetic wound closure compared with primary DFU fibroblasts.
30088952	3	47	theme	biologic	485:492	arg1	potential					494:502	the biologic potential	481:502	the biologic potential of iPSC-derived cells to treat DFUs	481:538	However, the biologic potential of iPSC-derived cells to treat DFUs has not, to our knowledge, been investigated.
30088952	9	48	dep	I.	1765:1766	arg1	Mooney					1786:1791	Mooney	1786:1791	Mooney	1786:1791	Taken together, our findings support the potential application of these iPSC-derived fibroblasts and 3D tissues to improve wound healing.-Kashpur, O., Smith, A., Gerami-Naini, B., Maione, A. G., Calabrese, R., Tellechea, A., Theocharidis, G., Liang, L., Pastar, I., Tomic-Canic, M., Mooney, D., Veves, A., Garlick, J. A. Differentiation of diabetic foot ulcer-derived induced pluripotent stem cells reveals distinct cellular and tissue phenotypes.
30088952	9	48	dep	I.	1765:1766	arg1	D.					1794:1795	D.	1794:1795	D.	1794:1795	Taken together, our findings support the potential application of these iPSC-derived fibroblasts and 3D tissues to improve wound healing.-Kashpur, O., Smith, A., Gerami-Naini, B., Maione, A. G., Calabrese, R., Tellechea, A., Theocharidis, G., Liang, L., Pastar, I., Tomic-Canic, M., Mooney, D., Veves, A., Garlick, J. A. Differentiation of diabetic foot ulcer-derived induced pluripotent stem cells reveals distinct cellular and tissue phenotypes.
30088952	9	48	dep	I.	1765:1766	arg1	Garlick					1809:1815	Garlick	1809:1815	Garlick	1809:1815	Taken together, our findings support the potential application of these iPSC-derived fibroblasts and 3D tissues to improve wound healing.-Kashpur, O., Smith, A., Gerami-Naini, B., Maione, A. G., Calabrese, R., Tellechea, A., Theocharidis, G., Liang, L., Pastar, I., Tomic-Canic, M., Mooney, D., Veves, A., Garlick, J. A. Differentiation of diabetic foot ulcer-derived induced pluripotent stem cells reveals distinct cellular and tissue phenotypes.
30088952	9	48	dep	I.	1765:1766	arg1	J.					1818:1819	J.	1818:1819	J.	1818:1819	Taken together, our findings support the potential application of these iPSC-derived fibroblasts and 3D tissues to improve wound healing.-Kashpur, O., Smith, A., Gerami-Naini, B., Maione, A. G., Calabrese, R., Tellechea, A., Theocharidis, G., Liang, L., Pastar, I., Tomic-Canic, M., Mooney, D., Veves, A., Garlick, J. A. Differentiation of diabetic foot ulcer-derived induced pluripotent stem cells reveals distinct cellular and tissue phenotypes.
30088952	9	48	dep	I.	1765:1766	arg1	A.					1821:1822	A.	1821:1822	A.	1821:1822	Taken together, our findings support the potential application of these iPSC-derived fibroblasts and 3D tissues to improve wound healing.-Kashpur, O., Smith, A., Gerami-Naini, B., Maione, A. G., Calabrese, R., Tellechea, A., Theocharidis, G., Liang, L., Pastar, I., Tomic-Canic, M., Mooney, D., Veves, A., Garlick, J. A. Differentiation of diabetic foot ulcer-derived induced pluripotent stem cells reveals distinct cellular and tissue phenotypes.
30088952	9	48	dep	I.	1765:1766	arg1	Veves					1798:1802	Veves	1798:1802	Veves	1798:1802	Taken together, our findings support the potential application of these iPSC-derived fibroblasts and 3D tissues to improve wound healing.-Kashpur, O., Smith, A., Gerami-Naini, B., Maione, A. G., Calabrese, R., Tellechea, A., Theocharidis, G., Liang, L., Pastar, I., Tomic-Canic, M., Mooney, D., Veves, A., Garlick, J. A. Differentiation of diabetic foot ulcer-derived induced pluripotent stem cells reveals distinct cellular and tissue phenotypes.
30088952	9	48	dep	I.	1765:1766	arg1	A.					1805:1806	A.	1805:1806	A.	1805:1806	Taken together, our findings support the potential application of these iPSC-derived fibroblasts and 3D tissues to improve wound healing.-Kashpur, O., Smith, A., Gerami-Naini, B., Maione, A. G., Calabrese, R., Tellechea, A., Theocharidis, G., Liang, L., Pastar, I., Tomic-Canic, M., Mooney, D., Veves, A., Garlick, J. A. Differentiation of diabetic foot ulcer-derived induced pluripotent stem cells reveals distinct cellular and tissue phenotypes.
30088952	9	48	dep	I.	1765:1766	arg1	O.					1650:1651	O.	1650:1651	O.	1650:1651	Taken together, our findings support the potential application of these iPSC-derived fibroblasts and 3D tissues to improve wound healing.-Kashpur, O., Smith, A., Gerami-Naini, B., Maione, A. G., Calabrese, R., Tellechea, A., Theocharidis, G., Liang, L., Pastar, I., Tomic-Canic, M., Mooney, D., Veves, A., Garlick, J. A. Differentiation of diabetic foot ulcer-derived induced pluripotent stem cells reveals distinct cellular and tissue phenotypes.
30088952	7	49	theme	parental	1262:1269	arg1	fibroblasts					1275:1285	the parental DFU fibroblasts	1258:1285	the parental DFU fibroblasts from which they were reprogrammed	1258:1319	iPSC-derived fibroblasts from DFUs displayed a unique biochemical composition and morphology when grown as 3-dimensional (3D), self-assembled extracellular matrix tissues, which were distinct from tissues fabricated using the parental DFU fibroblasts from which they were reprogrammed.
30088952	3	50	theme	cells	520:524	arg1	potential					494:502	the biologic potential	481:502	the biologic potential of iPSC-derived cells to treat DFUs	481:538	However, the biologic potential of iPSC-derived cells to treat DFUs has not, to our knowledge, been investigated.
30088952	4	51	from	characterization	631:646	arg1	patients					711:718	both diabetic and nondiabetic patients	681:718	both diabetic and nondiabetic patients	681:718	Toward that goal, we have performed detailed characterization of iPSC-derived fibroblasts from both diabetic and nondiabetic patients.
30088952	1	52	theme	cell-	239:243	arg1	therapies					262:270	novel cell- and tissue-based therapies	233:270	novel cell- and tissue-based therapies	233:270	Diabetic foot ulcers (DFUs) are a major complication of diabetes, and there is a critical need to develop novel cell- and tissue-based therapies to treat these chronic wounds.
30088952	5	53	dep	cells	912:916	arg1	were					895:898	were	895:898	were	895:898	Significantly, gene array and functional analyses reveal that iPSC-derived fibroblasts from both patients with and those without diabetes are more similar to each other than were the primary cells from which they were derived.
30088952	5	53	dep	cells	912:916	arg1	than					890:893	than	890:893	than	890:893	Significantly, gene array and functional analyses reveal that iPSC-derived fibroblasts from both patients with and those without diabetes are more similar to each other than were the primary cells from which they were derived.
30088952	1	54	theme	major	161:165	arg1	ulcers					141:146	Diabetic foot ulcers	127:146	Diabetic foot ulcers (DFUs)	127:153	Diabetic foot ulcers (DFUs) are a major complication of diabetes, and there is a critical need to develop novel cell- and tissue-based therapies to treat these chronic wounds.
30088952	1	54	theme	major	161:165	arg1	complication					167:178	a major complication	159:178	a major complication of diabetes	159:190	Diabetic foot ulcers (DFUs) are a major complication of diabetes, and there is a critical need to develop novel cell- and tissue-based therapies to treat these chronic wounds.
30088952	7	55	theme	biochemical	1090:1100	arg1	composition					1102:1112	a unique biochemical composition	1081:1112	a unique biochemical composition	1081:1112	iPSC-derived fibroblasts from DFUs displayed a unique biochemical composition and morphology when grown as 3-dimensional (3D), self-assembled extracellular matrix tissues, which were distinct from tissues fabricated using the parental DFU fibroblasts from which they were reprogrammed.
30088952	6	56	theme	iPSC-derived	948:959	arg1	fibroblasts					961:971	iPSC-derived fibroblasts	948:971	iPSC-derived fibroblasts	948:971	iPSC-derived fibroblasts showed improved migratory properties in 2-dimensional culture.
30088952	5	57	attach	derived	939:945	arg1	fibroblasts					796:806	iPSC-derived fibroblasts	783:806	iPSC-derived fibroblasts from both patients with and those without diabetes	783:857	Significantly, gene array and functional analyses reveal that iPSC-derived fibroblasts from both patients with and those without diabetes are more similar to each other than were the primary cells from which they were derived.
30088952	5	57	attach	derived	939:945	arg1	cells					912:916	the primary cells	900:916	the primary cells from which they were derived	900:945	Significantly, gene array and functional analyses reveal that iPSC-derived fibroblasts from both patients with and those without diabetes are more similar to each other than were the primary cells from which they were derived.
30088952	5	57	attach	derived	939:945	arg2	they					929:932	they	929:932	they	929:932	Significantly, gene array and functional analyses reveal that iPSC-derived fibroblasts from both patients with and those without diabetes are more similar to each other than were the primary cells from which they were derived.
30088952	4	58	link	iPSC-derived	651:662	arg1	fibroblasts					664:674	iPSC-derived fibroblasts	651:674	iPSC-derived fibroblasts from both diabetic and nondiabetic patients	651:718	Toward that goal, we have performed detailed characterization of iPSC-derived fibroblasts from both diabetic and nondiabetic patients.
30088952	8	59	dep	showed	1390:1395	arg1	facilitated					1429:1439	facilitated	1429:1439	facilitated diabetic wound closure compared with primary DFU fibroblasts	1429:1500	In vivo transplantation of 3D tissues with iPSC-derived fibroblasts showed they persisted in the wound and facilitated diabetic wound closure compared with primary DFU fibroblasts.
30088952	8	59	dep	showed	1390:1395	arg1	persisted					1402:1410	persisted	1402:1410	persisted in the wound	1402:1423	In vivo transplantation of 3D tissues with iPSC-derived fibroblasts showed they persisted in the wound and facilitated diabetic wound closure compared with primary DFU fibroblasts.
30088952	8	60	link	iPSC-derived	1365:1376	arg1	fibroblasts					1378:1388	iPSC-derived fibroblasts	1365:1388	iPSC-derived fibroblasts	1365:1388	In vivo transplantation of 3D tissues with iPSC-derived fibroblasts showed they persisted in the wound and facilitated diabetic wound closure compared with primary DFU fibroblasts.
30088952	8	61	theme	DFU	1486:1488	arg1	fibroblasts					1490:1500	primary DFU fibroblasts	1478:1500	primary DFU fibroblasts	1478:1500	In vivo transplantation of 3D tissues with iPSC-derived fibroblasts showed they persisted in the wound and facilitated diabetic wound closure compared with primary DFU fibroblasts.
30088952	4	62	theme	iPSC-derived	651:662	arg1	fibroblasts					664:674	iPSC-derived fibroblasts	651:674	iPSC-derived fibroblasts from both diabetic and nondiabetic patients	651:718	Toward that goal, we have performed detailed characterization of iPSC-derived fibroblasts from both diabetic and nondiabetic patients.
30088952	9	63	theme	diabetic	1843:1850	arg1	foot					1852:1855	diabetic foot	1843:1855	diabetic foot	1843:1855	Taken together, our findings support the potential application of these iPSC-derived fibroblasts and 3D tissues to improve wound healing.-Kashpur, O., Smith, A., Gerami-Naini, B., Maione, A. G., Calabrese, R., Tellechea, A., Theocharidis, G., Liang, L., Pastar, I., Tomic-Canic, M., Mooney, D., Veves, A., Garlick, J. A. Differentiation of diabetic foot ulcer-derived induced pluripotent stem cells reveals distinct cellular and tissue phenotypes.
30088952	2	64	theme	stem	323:326	arg1	iPSCs					335:339	iPSCs	335:339	iPSCs	335:339	Induced pluripotent stem cells (iPSCs) offer a replenishing source of allogeneic and autologous cell types that may be beneficial to improve DFU wound-healing outcomes.
30088952	2	64	theme	stem	323:326	arg1	cells					328:332	Induced pluripotent stem cells	303:332	Induced pluripotent stem cells (iPSCs)	303:340	Induced pluripotent stem cells (iPSCs) offer a replenishing source of allogeneic and autologous cell types that may be beneficial to improve DFU wound-healing outcomes.
30088952	6	65	link	iPSC-derived	948:959	arg1	fibroblasts					961:971	iPSC-derived fibroblasts	948:971	iPSC-derived fibroblasts	948:971	iPSC-derived fibroblasts showed improved migratory properties in 2-dimensional culture.
30088952	5	66	from	those	836:840	arg1	fibroblasts					796:806	iPSC-derived fibroblasts	783:806	iPSC-derived fibroblasts from both patients with and those without diabetes	783:857	Significantly, gene array and functional analyses reveal that iPSC-derived fibroblasts from both patients with and those without diabetes are more similar to each other than were the primary cells from which they were derived.
30088952	5	66	from	those	836:840	arg1	cells					912:916	the primary cells	900:916	the primary cells from which they were derived	900:945	Significantly, gene array and functional analyses reveal that iPSC-derived fibroblasts from both patients with and those without diabetes are more similar to each other than were the primary cells from which they were derived.
30088952	1	67	theme	Diabetic	127:134	arg1	ulcers					141:146	Diabetic foot ulcers	127:146	Diabetic foot ulcers (DFUs)	127:153	Diabetic foot ulcers (DFUs) are a major complication of diabetes, and there is a critical need to develop novel cell- and tissue-based therapies to treat these chronic wounds.
30088952	1	67	theme	Diabetic	127:134	arg1	complication					167:178	a major complication	159:178	a major complication of diabetes	159:190	Diabetic foot ulcers (DFUs) are a major complication of diabetes, and there is a critical need to develop novel cell- and tissue-based therapies to treat these chronic wounds.
30088952	1	67	theme	Diabetic	127:134	arg1	DFUs					149:152	DFUs	149:152	DFUs	149:152	Diabetic foot ulcers (DFUs) are a major complication of diabetes, and there is a critical need to develop novel cell- and tissue-based therapies to treat these chronic wounds.
30088952	9	68	theme	pluripotent	1879:1889	arg1	cells					1896:1900	induced pluripotent stem cells	1871:1900	induced pluripotent stem cells	1871:1900	Taken together, our findings support the potential application of these iPSC-derived fibroblasts and 3D tissues to improve wound healing.-Kashpur, O., Smith, A., Gerami-Naini, B., Maione, A. G., Calabrese, R., Tellechea, A., Theocharidis, G., Liang, L., Pastar, I., Tomic-Canic, M., Mooney, D., Veves, A., Garlick, J. A. Differentiation of diabetic foot ulcer-derived induced pluripotent stem cells reveals distinct cellular and tissue phenotypes.
30088952	4	69	theme	diabetic	686:693	arg1	patients					711:718	both diabetic and nondiabetic patients	681:718	both diabetic and nondiabetic patients	681:718	Toward that goal, we have performed detailed characterization of iPSC-derived fibroblasts from both diabetic and nondiabetic patients.
30088952	5	70	link	iPSC-derived	783:794	arg1	fibroblasts					796:806	iPSC-derived fibroblasts	783:806	iPSC-derived fibroblasts from both patients with and those without diabetes	783:857	Significantly, gene array and functional analyses reveal that iPSC-derived fibroblasts from both patients with and those without diabetes are more similar to each other than were the primary cells from which they were derived.
30088952	5	70	link	iPSC-derived	783:794	arg1	cells					912:916	the primary cells	900:916	the primary cells from which they were derived	900:945	Significantly, gene array and functional analyses reveal that iPSC-derived fibroblasts from both patients with and those without diabetes are more similar to each other than were the primary cells from which they were derived.
30088952	9	71	dep	foot	1852:1855	arg1	improve					1618:1624	improve	1618:1624	to improve wound healing.-Kashpur, O., Smith, A., Gerami-Naini, B., Maione, A. G., Calabrese, R., Tellechea, A., Theocharidis, G., Liang, L., Pastar, I., Tomic-Canic, M., Mooney, D., Veves, A., Garlick, J. A.	1615:1822	Taken together, our findings support the potential application of these iPSC-derived fibroblasts and 3D tissues to improve wound healing.-Kashpur, O., Smith, A., Gerami-Naini, B., Maione, A. G., Calabrese, R., Tellechea, A., Theocharidis, G., Liang, L., Pastar, I., Tomic-Canic, M., Mooney, D., Veves, A., Garlick, J. A. Differentiation of diabetic foot ulcer-derived induced pluripotent stem cells reveals distinct cellular and tissue phenotypes.
30088952	9	71	dep	foot	1852:1855	arg1	Differentiation					1824:1838	Differentiation	1824:1838	Differentiation	1824:1838	Taken together, our findings support the potential application of these iPSC-derived fibroblasts and 3D tissues to improve wound healing.-Kashpur, O., Smith, A., Gerami-Naini, B., Maione, A. G., Calabrese, R., Tellechea, A., Theocharidis, G., Liang, L., Pastar, I., Tomic-Canic, M., Mooney, D., Veves, A., Garlick, J. A. Differentiation of diabetic foot ulcer-derived induced pluripotent stem cells reveals distinct cellular and tissue phenotypes.
30088952	9	71	dep	foot	1852:1855	arg1	cells					1896:1900	induced pluripotent stem cells	1871:1900	induced pluripotent stem cells	1871:1900	Taken together, our findings support the potential application of these iPSC-derived fibroblasts and 3D tissues to improve wound healing.-Kashpur, O., Smith, A., Gerami-Naini, B., Maione, A. G., Calabrese, R., Tellechea, A., Theocharidis, G., Liang, L., Pastar, I., Tomic-Canic, M., Mooney, D., Veves, A., Garlick, J. A. Differentiation of diabetic foot ulcer-derived induced pluripotent stem cells reveals distinct cellular and tissue phenotypes.
30088952	6	72	theme	migratory	989:997	arg1	properties					999:1008	improved migratory properties	980:1008	improved migratory properties	980:1008	iPSC-derived fibroblasts showed improved migratory properties in 2-dimensional culture.
30088952	2	73	theme	wound-healing	448:460	arg1	outcomes					462:469	DFU wound-healing outcomes	444:469	DFU wound-healing outcomes	444:469	Induced pluripotent stem cells (iPSCs) offer a replenishing source of allogeneic and autologous cell types that may be beneficial to improve DFU wound-healing outcomes.
30088952	8	74	theme	3D	1349:1350	arg1	tissues					1352:1358	3D tissues	1349:1358	3D tissues	1349:1358	In vivo transplantation of 3D tissues with iPSC-derived fibroblasts showed they persisted in the wound and facilitated diabetic wound closure compared with primary DFU fibroblasts.
30088952	0	75	theme	tissue	108:113	arg1	phenotypes					115:124	distinct cellular and tissue phenotypes	86:124	phenotypes	115:124	Differentiation of diabetic foot ulcer-derived induced pluripotent stem cells reveals distinct cellular and tissue phenotypes.
30088952	3	76	link	iPSC-derived	507:518	arg1	cells					520:524	iPSC-derived cells	507:524	iPSC-derived cells	507:524	However, the biologic potential of iPSC-derived cells to treat DFUs has not, to our knowledge, been investigated.
30088952	9	77	theme	3D	1604:1605	arg1	tissues					1607:1613	3D tissues	1604:1613	3D tissues	1604:1613	Taken together, our findings support the potential application of these iPSC-derived fibroblasts and 3D tissues to improve wound healing.-Kashpur, O., Smith, A., Gerami-Naini, B., Maione, A. G., Calabrese, R., Tellechea, A., Theocharidis, G., Liang, L., Pastar, I., Tomic-Canic, M., Mooney, D., Veves, A., Garlick, J. A. Differentiation of diabetic foot ulcer-derived induced pluripotent stem cells reveals distinct cellular and tissue phenotypes.
30088952	5	78	theme	iPSC-derived	783:794	arg1	fibroblasts					796:806	iPSC-derived fibroblasts	783:806	iPSC-derived fibroblasts from both patients with and those without diabetes	783:857	Significantly, gene array and functional analyses reveal that iPSC-derived fibroblasts from both patients with and those without diabetes are more similar to each other than were the primary cells from which they were derived.
30088952	5	78	theme	iPSC-derived	783:794	arg1	cells					912:916	the primary cells	900:916	the primary cells from which they were derived	900:945	Significantly, gene array and functional analyses reveal that iPSC-derived fibroblasts from both patients with and those without diabetes are more similar to each other than were the primary cells from which they were derived.
30088952	4	79	theme	nondiabetic	699:709	arg1	patients					711:718	both diabetic and nondiabetic patients	681:718	both diabetic and nondiabetic patients	681:718	Toward that goal, we have performed detailed characterization of iPSC-derived fibroblasts from both diabetic and nondiabetic patients.
30088952	0	80	theme	foot	28:31	arg1	cells					72:76	diabetic foot ulcer-derived induced pluripotent stem cells	19:76	diabetic foot ulcer-derived induced pluripotent stem cells	19:76	Differentiation of diabetic foot ulcer-derived induced pluripotent stem cells reveals distinct cellular and tissue phenotypes.
30088952	2	81	theme	types	404:408	arg1	source					363:368	a replenishing source	348:368	a replenishing source of allogeneic and autologous cell types that may be beneficial to improve DFU wound-healing outcomes	348:469	Induced pluripotent stem cells (iPSCs) offer a replenishing source of allogeneic and autologous cell types that may be beneficial to improve DFU wound-healing outcomes.
30088952	2	81	theme	types	404:408	arg1	beneficial					422:431	beneficial	422:431	beneficial	422:431	Induced pluripotent stem cells (iPSCs) offer a replenishing source of allogeneic and autologous cell types that may be beneficial to improve DFU wound-healing outcomes.
30088952	2	82	theme	autologous	388:397	arg1	types					404:408	allogeneic and autologous cell types	373:408	allogeneic and autologous cell types	373:408	Induced pluripotent stem cells (iPSCs) offer a replenishing source of allogeneic and autologous cell types that may be beneficial to improve DFU wound-healing outcomes.
30088952	5	83	theme	functional	751:760	arg1	analyses					762:769	functional analyses	751:769	functional analyses	751:769	Significantly, gene array and functional analyses reveal that iPSC-derived fibroblasts from both patients with and those without diabetes are more similar to each other than were the primary cells from which they were derived.
30088952	2	84	theme	allogeneic	373:382	arg1	types					404:408	allogeneic and autologous cell types	373:408	allogeneic and autologous cell types	373:408	Induced pluripotent stem cells (iPSCs) offer a replenishing source of allogeneic and autologous cell types that may be beneficial to improve DFU wound-healing outcomes.
30088952	0	85	theme	stem	67:70	arg1	cells					72:76	diabetic foot ulcer-derived induced pluripotent stem cells	19:76	diabetic foot ulcer-derived induced pluripotent stem cells	19:76	Differentiation of diabetic foot ulcer-derived induced pluripotent stem cells reveals distinct cellular and tissue phenotypes.
30088952	1	86	theme	chronic	287:293	arg1	wounds					295:300	these chronic wounds	281:300	these chronic wounds	281:300	Diabetic foot ulcers (DFUs) are a major complication of diabetes, and there is a critical need to develop novel cell- and tissue-based therapies to treat these chronic wounds.
30088952	7	87	link	iPSC-derived	1036:1047	arg1	fibroblasts					1049:1059	iPSC-derived fibroblasts	1036:1059	iPSC-derived fibroblasts from DFUs	1036:1069	iPSC-derived fibroblasts from DFUs displayed a unique biochemical composition and morphology when grown as 3-dimensional (3D), self-assembled extracellular matrix tissues, which were distinct from tissues fabricated using the parental DFU fibroblasts from which they were reprogrammed.
30088952	8	88	theme	wound	1450:1454	arg1	closure					1456:1462	diabetic wound closure	1441:1462	diabetic wound closure	1441:1462	In vivo transplantation of 3D tissues with iPSC-derived fibroblasts showed they persisted in the wound and facilitated diabetic wound closure compared with primary DFU fibroblasts.
30088952	2	89	theme	Induced	303:309	arg1	iPSCs					335:339	iPSCs	335:339	iPSCs	335:339	Induced pluripotent stem cells (iPSCs) offer a replenishing source of allogeneic and autologous cell types that may be beneficial to improve DFU wound-healing outcomes.
30088952	2	89	theme	Induced	303:309	arg1	cells					328:332	Induced pluripotent stem cells	303:332	Induced pluripotent stem cells (iPSCs)	303:340	Induced pluripotent stem cells (iPSCs) offer a replenishing source of allogeneic and autologous cell types that may be beneficial to improve DFU wound-healing outcomes.
30088952	1	90	theme	critical	208:215	arg1	need					217:220	a critical need	206:220	a critical need to develop novel cell- and tissue-based therapies to treat these chronic wounds	206:300	Diabetic foot ulcers (DFUs) are a major complication of diabetes, and there is a critical need to develop novel cell- and tissue-based therapies to treat these chronic wounds.
30088952	7	91	theme	DFU	1271:1273	arg1	fibroblasts					1275:1285	the parental DFU fibroblasts	1258:1285	the parental DFU fibroblasts from which they were reprogrammed	1258:1319	iPSC-derived fibroblasts from DFUs displayed a unique biochemical composition and morphology when grown as 3-dimensional (3D), self-assembled extracellular matrix tissues, which were distinct from tissues fabricated using the parental DFU fibroblasts from which they were reprogrammed.
30088952	7	92	theme	matrix	1192:1197	arg1	tissues					1199:1205	3-dimensional (3D), self-assembled extracellular matrix tissues	1143:1205	tissues	1199:1205	iPSC-derived fibroblasts from DFUs displayed a unique biochemical composition and morphology when grown as 3-dimensional (3D), self-assembled extracellular matrix tissues, which were distinct from tissues fabricated using the parental DFU fibroblasts from which they were reprogrammed.
29458471	3	0	theme	%	433:433	arg1	%					459:459	optimum 3.5 %	447:459	optimum 3.5 %	447:459	The strain grew at 4-34 °C (optimum 28 °C), in the presence of 0.5-9.5 % (w/v) NaCl (optimum 3.5 %) and at pH 4.5-10.0 (optimum pH 7.5).
29458471	3	0	theme	%	433:433	arg1	NaCl					441:444	0.5-9.5 % (w/v) NaCl	425:444	0.5-9.5 % (w/v) NaCl (optimum 3.5 %)	425:460	The strain grew at 4-34 °C (optimum 28 °C), in the presence of 0.5-9.5 % (w/v) NaCl (optimum 3.5 %) and at pH 4.5-10.0 (optimum pH 7.5).
29458471	6	1	theme	taeanensis	751:760	arg1	22008T					766:771	L. taeanensis DSM 22008T	748:771	L. taeanensis DSM 22008T	748:771	DNA-DNA hybridization of strain MAA42T with L. taeanensis DSM 22008T resulted in values of 4.7 % (reciprocal 17.7 %).
29458471	2	2	theme	motile	147:152	arg1	strain					181:186	strain MAA42T	181:193	strain MAA42T	181:193	A bright-orange-pigmented, Gram-stain-negative, motile, and rod-shaped bacterium, strain MAA42T, was isolated from a marine sponge of the genus Haliclona, which is in long-time culture in a marine aquarium system at the Justus Liebig University Giessen, Germany.
29458471	2	2	theme	motile	147:152	arg1	bacterium					170:178	A bright-orange-pigmented, Gram-stain-negative, motile, and rod-shaped bacterium	99:178	A bright-orange-pigmented, Gram-stain-negative, motile, and rod-shaped bacterium	99:178	A bright-orange-pigmented, Gram-stain-negative, motile, and rod-shaped bacterium, strain MAA42T, was isolated from a marine sponge of the genus Haliclona, which is in long-time culture in a marine aquarium system at the Justus Liebig University Giessen, Germany.
29458471	7	3	theme	 1	897:898	arg1	2-OH					900:903	 1 2-OH	897:903	C18 : 1 2-OH (17.4 %)	892:912	Major cellular fatty acids of strain MAA42T were C18 : 1ω7c (66.2 %), C18 : 1 2-OH (17.4 %), and C18 : 0 (14.1 %).
29458471	7	3	theme	 1	897:898	arg1	%					911:911	17.4 %	906:911	17.4 %	906:911	Major cellular fatty acids of strain MAA42T were C18 : 1ω7c (66.2 %), C18 : 1 2-OH (17.4 %), and C18 : 0 (14.1 %).
29458471	3	4	theme	optimum	447:453	arg1	%					459:459	optimum 3.5 %	447:459	optimum 3.5 %	447:459	The strain grew at 4-34 °C (optimum 28 °C), in the presence of 0.5-9.5 % (w/v) NaCl (optimum 3.5 %) and at pH 4.5-10.0 (optimum pH 7.5).
29458471	3	4	theme	optimum	447:453	arg1	NaCl					441:444	0.5-9.5 % (w/v) NaCl	425:444	0.5-9.5 % (w/v) NaCl (optimum 3.5 %)	425:460	The strain grew at 4-34 °C (optimum 28 °C), in the presence of 0.5-9.5 % (w/v) NaCl (optimum 3.5 %) and at pH 4.5-10.0 (optimum pH 7.5).
29458471	4	5	theme	highest	524:530	arg1	similarity					555:564	the highest 16S rRNA gene sequence similarity	520:564	the highest 16S rRNA gene sequence similarity (98.1 %)	520:573	Strain MAA42T shared the highest 16S rRNA gene sequence similarity (98.1 %) with the type strain of Litorimonas taeanensis.
29458471	4	5	theme	highest	524:530	arg1	%					572:572	98.1 %	567:572	98.1 %	567:572	Strain MAA42T shared the highest 16S rRNA gene sequence similarity (98.1 %) with the type strain of Litorimonas taeanensis.
29458471	7	6	dep	C18 	871:874	arg1	 1ω7c					876:880	 1ω7c	876:880	 1ω7c	876:880	Major cellular fatty acids of strain MAA42T were C18 : 1ω7c (66.2 %), C18 : 1 2-OH (17.4 %), and C18 : 0 (14.1 %).
29458471	7	6	dep	C18 	871:874	arg1	%					888:888	66.2 %	883:888	66.2 %	883:888	Major cellular fatty acids of strain MAA42T were C18 : 1ω7c (66.2 %), C18 : 1 2-OH (17.4 %), and C18 : 0 (14.1 %).
29458471	2	7	theme	Gram-stain-negative	126:144	arg1	strain					181:186	strain MAA42T	181:193	strain MAA42T	181:193	A bright-orange-pigmented, Gram-stain-negative, motile, and rod-shaped bacterium, strain MAA42T, was isolated from a marine sponge of the genus Haliclona, which is in long-time culture in a marine aquarium system at the Justus Liebig University Giessen, Germany.
29458471	2	7	theme	Gram-stain-negative	126:144	arg1	bacterium					170:178	A bright-orange-pigmented, Gram-stain-negative, motile, and rod-shaped bacterium	99:178	A bright-orange-pigmented, Gram-stain-negative, motile, and rod-shaped bacterium	99:178	A bright-orange-pigmented, Gram-stain-negative, motile, and rod-shaped bacterium, strain MAA42T, was isolated from a marine sponge of the genus Haliclona, which is in long-time culture in a marine aquarium system at the Justus Liebig University Giessen, Germany.
29458471	3	8	theme	NaCl	441:444	arg1	presence					413:420	the presence	409:420	the presence of 0.5-9.5 % (w/v) NaCl (optimum 3.5 %)	409:460	The strain grew at 4-34 °C (optimum 28 °C), in the presence of 0.5-9.5 % (w/v) NaCl (optimum 3.5 %) and at pH 4.5-10.0 (optimum pH 7.5).
29458471	6	9	theme	strain	729:734	arg1	MAA42T					736:741	strain MAA42T	729:741	strain MAA42T	729:741	DNA-DNA hybridization of strain MAA42T with L. taeanensis DSM 22008T resulted in values of 4.7 % (reciprocal 17.7 %).
29458471	4	10	theme	rRNA	536:539	arg1	similarity					555:564	the highest 16S rRNA gene sequence similarity	520:564	the highest 16S rRNA gene sequence similarity (98.1 %)	520:573	Strain MAA42T shared the highest 16S rRNA gene sequence similarity (98.1 %) with the type strain of Litorimonas taeanensis.
29458471	4	10	theme	rRNA	536:539	arg1	%					572:572	98.1 %	567:572	98.1 %	567:572	Strain MAA42T shared the highest 16S rRNA gene sequence similarity (98.1 %) with the type strain of Litorimonas taeanensis.
29458471	2	11	theme	long-time	266:274	arg1	culture					276:282	long-time culture	266:282	long-time culture in a marine aquarium system at the Justus Liebig University Giessen, Germany	266:359	A bright-orange-pigmented, Gram-stain-negative, motile, and rod-shaped bacterium, strain MAA42T, was isolated from a marine sponge of the genus Haliclona, which is in long-time culture in a marine aquarium system at the Justus Liebig University Giessen, Germany.
29458471	6	12	theme	reciprocal	802:811	arg1	%					818:818	reciprocal 17.7 %	802:818	reciprocal 17.7 %	802:818	DNA-DNA hybridization of strain MAA42T with L. taeanensis DSM 22008T resulted in values of 4.7 % (reciprocal 17.7 %).
29458471	6	12	theme	reciprocal	802:811	arg1	%					799:799	4.7 %	795:799	4.7 % (reciprocal 17.7 %)	795:819	DNA-DNA hybridization of strain MAA42T with L. taeanensis DSM 22008T resulted in values of 4.7 % (reciprocal 17.7 %).
29458471	4	13	theme	Strain	499:504	arg1	MAA42T					506:511	Strain MAA42T	499:511	Strain MAA42T	499:511	Strain MAA42T shared the highest 16S rRNA gene sequence similarity (98.1 %) with the type strain of Litorimonas taeanensis.
29458471	13	14	theme	name	1544:1547	arg1	haliclonae					1561:1570	the name Litorimonas haliclonae	1540:1570	the name Litorimonas haliclonae	1540:1570	Based on the genotypic, chemotaxonomic, and phenotypic analyses, strain MAA42T represents a novel species of the genus Litorimonas, for which the name Litorimonas haliclonae is proposed.
29458471	6	15	theme	%	799:799	arg1	values					785:790	values	785:790	values of 4.7 % (reciprocal 17.7 %)	785:819	DNA-DNA hybridization of strain MAA42T with L. taeanensis DSM 22008T resulted in values of 4.7 % (reciprocal 17.7 %).
29458471	13	16	theme	genus	1511:1515	arg1	Litorimonas					1517:1527	the genus Litorimonas	1507:1527	the genus Litorimonas	1507:1527	Based on the genotypic, chemotaxonomic, and phenotypic analyses, strain MAA42T represents a novel species of the genus Litorimonas, for which the name Litorimonas haliclonae is proposed.
29458471	5	17	theme	type	674:677	arg1	strains					679:685	all other closely related type strains	648:685	all other closely related type strains	648:685	Sequence similarities to all other closely related type strains were below 97 %.
29458471	8	18	from	pattern	981:987	arg1	predominant					952:962	predominant	952:962	predominant	952:962	Spermidine was predominant in the polyamine pattern, and ubiquinone Q-10 was the major respiratory quinone.
29458471	12	19	theme	DNA	1367:1369	arg1	G+C content					1371:1381	The genomic DNA G+C content	1355:1381	The genomic DNA G+C content	1355:1381	The genomic DNA G+C content was 52.8 mol%.
29458471	12	19	theme	DNA	1367:1369	arg1	%					1395:1395	52.8 mol%	1387:1395	52.8 mol%	1387:1395	The genomic DNA G+C content was 52.8 mol%.
29458471	7	20	dep	C18 	892:895	arg1	2-OH					900:903	 1 2-OH	897:903	C18 : 1 2-OH (17.4 %)	892:912	Major cellular fatty acids of strain MAA42T were C18 : 1ω7c (66.2 %), C18 : 1 2-OH (17.4 %), and C18 : 0 (14.1 %).
29458471	7	20	dep	C18 	892:895	arg1	%					911:911	17.4 %	906:911	17.4 %	906:911	Major cellular fatty acids of strain MAA42T were C18 : 1ω7c (66.2 %), C18 : 1 2-OH (17.4 %), and C18 : 0 (14.1 %).
29458471	7	21	theme	cellular	828:835	arg1	acids					843:847	Major cellular fatty acids	822:847	Major cellular fatty acids of strain MAA42T	822:864	Major cellular fatty acids of strain MAA42T were C18 : 1ω7c (66.2 %), C18 : 1 2-OH (17.4 %), and C18 : 0 (14.1 %).
29458471	8	22	theme	respiratory	1024:1034	arg1	quinone					1036:1042	the major respiratory quinone	1014:1042	the major respiratory quinone	1014:1042	Spermidine was predominant in the polyamine pattern, and ubiquinone Q-10 was the major respiratory quinone.
29458471	8	22	theme	respiratory	1024:1034	arg1	Q-10					1005:1008	ubiquinone Q-10	994:1008	ubiquinone Q-10	994:1008	Spermidine was predominant in the polyamine pattern, and ubiquinone Q-10 was the major respiratory quinone.
29458471	7	23	theme	MAA42T	859:864	arg1	acids					843:847	Major cellular fatty acids	822:847	Major cellular fatty acids of strain MAA42T	822:864	Major cellular fatty acids of strain MAA42T were C18 : 1ω7c (66.2 %), C18 : 1 2-OH (17.4 %), and C18 : 0 (14.1 %).
29458471	14	24	theme	8709T=CIP	1617:1625	arg1	29765T					1639:1644	=CCM 8709T=CIP 111178T=LMG 29765T	1612:1644	=CCM 8709T=CIP 111178T=LMG 29765T	1612:1644	The type strain is MAA42T (=CCM 8709T=CIP 111178T=LMG 29765T).
29458471	14	24	theme	8709T=CIP	1617:1625	arg1	MAA42T					1604:1609	MAA42T	1604:1609	MAA42T (=CCM 8709T=CIP 111178T=LMG 29765T)	1604:1645	The type strain is MAA42T (=CCM 8709T=CIP 111178T=LMG 29765T).
29458471	4	25	theme	sequence	546:553	arg1	similarity					555:564	the highest 16S rRNA gene sequence similarity	520:564	the highest 16S rRNA gene sequence similarity (98.1 %)	520:573	Strain MAA42T shared the highest 16S rRNA gene sequence similarity (98.1 %) with the type strain of Litorimonas taeanensis.
29458471	4	25	theme	sequence	546:553	arg1	%					572:572	98.1 %	567:572	98.1 %	567:572	Strain MAA42T shared the highest 16S rRNA gene sequence similarity (98.1 %) with the type strain of Litorimonas taeanensis.
29458471	2	26	theme	marine	216:221	arg1	sponge					223:228	a marine sponge	214:228	a marine sponge	214:228	A bright-orange-pigmented, Gram-stain-negative, motile, and rod-shaped bacterium, strain MAA42T, was isolated from a marine sponge of the genus Haliclona, which is in long-time culture in a marine aquarium system at the Justus Liebig University Giessen, Germany.
29458471	8	27	from	predominant	952:962	arg1	pattern					981:987	the polyamine pattern	967:987	the polyamine pattern	967:987	Spermidine was predominant in the polyamine pattern, and ubiquinone Q-10 was the major respiratory quinone.
29458471	5	28	theme	Sequence	623:630	arg1	similarities					632:643	Sequence similarities	623:643	Sequence similarities to all other closely related type strains	623:685	Sequence similarities to all other closely related type strains were below 97 %.
29458471	2	29	from	culture	276:282	arg1	system					305:310	a marine aquarium system	287:310	a marine aquarium system	287:310	A bright-orange-pigmented, Gram-stain-negative, motile, and rod-shaped bacterium, strain MAA42T, was isolated from a marine sponge of the genus Haliclona, which is in long-time culture in a marine aquarium system at the Justus Liebig University Giessen, Germany.
29458471	4	30	theme	taeanensis	611:620	arg1	strain					589:594	the type strain	580:594	the type strain of Litorimonas taeanensis	580:620	Strain MAA42T shared the highest 16S rRNA gene sequence similarity (98.1 %) with the type strain of Litorimonas taeanensis.
29458471	11	31	theme	diamino	1291:1297	arg1	acid					1299:1302	The diagnostic diamino acid	1276:1302	The diagnostic diamino acid of the peptidoglycan	1276:1323	The diagnostic diamino acid of the peptidoglycan was meso-diaminopimelic acid.
29458471	11	31	theme	diamino	1291:1297	arg1	acid					1349:1352	meso-diaminopimelic acid	1329:1352	meso-diaminopimelic acid	1329:1352	The diagnostic diamino acid of the peptidoglycan was meso-diaminopimelic acid.
29458471	2	32	attach	isolated	200:207	arg1	sponge					223:228	a marine sponge	214:228	a marine sponge	214:228	A bright-orange-pigmented, Gram-stain-negative, motile, and rod-shaped bacterium, strain MAA42T, was isolated from a marine sponge of the genus Haliclona, which is in long-time culture in a marine aquarium system at the Justus Liebig University Giessen, Germany.
29458471	2	32	attach	isolated	200:207	arg2	strain					181:186	strain MAA42T	181:193	strain MAA42T	181:193	A bright-orange-pigmented, Gram-stain-negative, motile, and rod-shaped bacterium, strain MAA42T, was isolated from a marine sponge of the genus Haliclona, which is in long-time culture in a marine aquarium system at the Justus Liebig University Giessen, Germany.
29458471	2	32	attach	isolated	200:207	arg2	bacterium					170:178	A bright-orange-pigmented, Gram-stain-negative, motile, and rod-shaped bacterium	99:178	A bright-orange-pigmented, Gram-stain-negative, motile, and rod-shaped bacterium	99:178	A bright-orange-pigmented, Gram-stain-negative, motile, and rod-shaped bacterium, strain MAA42T, was isolated from a marine sponge of the genus Haliclona, which is in long-time culture in a marine aquarium system at the Justus Liebig University Giessen, Germany.
29458471	1	33	theme	marine	61:66	arg1	sponge					68:73	a marine sponge	59:73	a marine sponge of the genus Haliclona	59:96	nov., isolated from a marine sponge of the genus Haliclona.
29458471	9	34	dep	compounds	1089:1097	arg1	compounds					1089:1097	the major compounds	1079:1097	the major compounds phosphatidylglycerol, monoglycosyldiglyceride, three unidentified phospholipids, and one unidentified glycolipid	1079:1210	The polar lipid profile contained the major compounds phosphatidylglycerol, monoglycosyldiglyceride, three unidentified phospholipids, and one unidentified glycolipid.
29458471	9	34	dep	compounds	1089:1097	arg1	monoglycosyldiglyceride					1121:1143	monoglycosyldiglyceride	1121:1143	monoglycosyldiglyceride	1121:1143	The polar lipid profile contained the major compounds phosphatidylglycerol, monoglycosyldiglyceride, three unidentified phospholipids, and one unidentified glycolipid.
29458471	9	34	dep	compounds	1089:1097	arg1	phosphatidylglycerol					1099:1118	phosphatidylglycerol	1099:1118	phosphatidylglycerol	1099:1118	The polar lipid profile contained the major compounds phosphatidylglycerol, monoglycosyldiglyceride, three unidentified phospholipids, and one unidentified glycolipid.
29458471	9	34	dep	compounds	1089:1097	arg1	glycolipid					1201:1210	one unidentified glycolipid	1184:1210	one unidentified glycolipid	1184:1210	The polar lipid profile contained the major compounds phosphatidylglycerol, monoglycosyldiglyceride, three unidentified phospholipids, and one unidentified glycolipid.
29458471	9	34	dep	compounds	1089:1097	arg1	phospholipids					1165:1177	three unidentified phospholipids	1146:1177	three unidentified phospholipids	1146:1177	The polar lipid profile contained the major compounds phosphatidylglycerol, monoglycosyldiglyceride, three unidentified phospholipids, and one unidentified glycolipid.
29458471	4	35	theme	type	584:587	arg1	strain					589:594	the type strain	580:594	the type strain of Litorimonas taeanensis	580:620	Strain MAA42T shared the highest 16S rRNA gene sequence similarity (98.1 %) with the type strain of Litorimonas taeanensis.
29458471	5	36	theme	related	666:672	arg1	strains					679:685	all other closely related type strains	648:685	all other closely related type strains	648:685	Sequence similarities to all other closely related type strains were below 97 %.
29458471	9	37	theme	lipid	1055:1059	arg1	profile					1061:1067	The polar lipid profile	1045:1067	The polar lipid profile	1045:1067	The polar lipid profile contained the major compounds phosphatidylglycerol, monoglycosyldiglyceride, three unidentified phospholipids, and one unidentified glycolipid.
29458471	6	38	with	hybridization	712:724	arg1	22008T					766:771	L. taeanensis DSM 22008T	748:771	L. taeanensis DSM 22008T	748:771	DNA-DNA hybridization of strain MAA42T with L. taeanensis DSM 22008T resulted in values of 4.7 % (reciprocal 17.7 %).
29458471	0	39	theme	Litorimonas	12:22	arg1	sp					35:36	Litorimonas haliclonae sp	12:36	Litorimonas haliclonae sp	12:36	Proposal of Litorimonas haliclonae sp.
29458471	1	40	theme	genus	82:86	arg1	Haliclona					88:96	the genus Haliclona	78:96	the genus Haliclona	78:96	nov., isolated from a marine sponge of the genus Haliclona.
29458471	13	41	theme	phenotypic	1442:1451	arg1	analyses					1453:1460	the genotypic, chemotaxonomic, and phenotypic analyses	1407:1460	the genotypic, chemotaxonomic, and phenotypic analyses	1407:1460	Based on the genotypic, chemotaxonomic, and phenotypic analyses, strain MAA42T represents a novel species of the genus Litorimonas, for which the name Litorimonas haliclonae is proposed.
29458471	2	42	theme	rod-shaped	159:168	arg1	strain					181:186	strain MAA42T	181:193	strain MAA42T	181:193	A bright-orange-pigmented, Gram-stain-negative, motile, and rod-shaped bacterium, strain MAA42T, was isolated from a marine sponge of the genus Haliclona, which is in long-time culture in a marine aquarium system at the Justus Liebig University Giessen, Germany.
29458471	2	42	theme	rod-shaped	159:168	arg1	bacterium					170:178	A bright-orange-pigmented, Gram-stain-negative, motile, and rod-shaped bacterium	99:178	A bright-orange-pigmented, Gram-stain-negative, motile, and rod-shaped bacterium	99:178	A bright-orange-pigmented, Gram-stain-negative, motile, and rod-shaped bacterium, strain MAA42T, was isolated from a marine sponge of the genus Haliclona, which is in long-time culture in a marine aquarium system at the Justus Liebig University Giessen, Germany.
29458471	3	43	theme	optimum	390:396	arg1	4-34 °C					381:387	4-34 °C	381:387	4-34 °C (optimum 28 °C)	381:403	The strain grew at 4-34 °C (optimum 28 °C), in the presence of 0.5-9.5 % (w/v) NaCl (optimum 3.5 %) and at pH 4.5-10.0 (optimum pH 7.5).
29458471	3	43	theme	optimum	390:396	arg1	28 °C					398:402	optimum 28 °C	390:402	optimum 28 °C	390:402	The strain grew at 4-34 °C (optimum 28 °C), in the presence of 0.5-9.5 % (w/v) NaCl (optimum 3.5 %) and at pH 4.5-10.0 (optimum pH 7.5).
29458471	0	44	theme	sp	35:36	arg1	Proposal					0:7	Proposal	0:7	Proposal of Litorimonas haliclonae sp.	0:37	Proposal of Litorimonas haliclonae sp.
29458471	9	45	theme	major	1083:1087	arg1	compounds					1089:1097	the major compounds	1079:1097	the major compounds phosphatidylglycerol, monoglycosyldiglyceride, three unidentified phospholipids, and one unidentified glycolipid	1079:1210	The polar lipid profile contained the major compounds phosphatidylglycerol, monoglycosyldiglyceride, three unidentified phospholipids, and one unidentified glycolipid.
29458471	9	45	theme	major	1083:1087	arg1	monoglycosyldiglyceride					1121:1143	monoglycosyldiglyceride	1121:1143	monoglycosyldiglyceride	1121:1143	The polar lipid profile contained the major compounds phosphatidylglycerol, monoglycosyldiglyceride, three unidentified phospholipids, and one unidentified glycolipid.
29458471	9	45	theme	major	1083:1087	arg1	phosphatidylglycerol					1099:1118	phosphatidylglycerol	1099:1118	phosphatidylglycerol	1099:1118	The polar lipid profile contained the major compounds phosphatidylglycerol, monoglycosyldiglyceride, three unidentified phospholipids, and one unidentified glycolipid.
29458471	9	45	theme	major	1083:1087	arg1	glycolipid					1201:1210	one unidentified glycolipid	1184:1210	one unidentified glycolipid	1184:1210	The polar lipid profile contained the major compounds phosphatidylglycerol, monoglycosyldiglyceride, three unidentified phospholipids, and one unidentified glycolipid.
29458471	6	46	theme	DNA-DNA	704:710	arg1	hybridization					712:724	DNA-DNA hybridization	704:724	DNA-DNA hybridization of strain MAA42T with L. taeanensis DSM 22008T	704:771	DNA-DNA hybridization of strain MAA42T with L. taeanensis DSM 22008T resulted in values of 4.7 % (reciprocal 17.7 %).
29458471	9	47	contain	contained	1069:1077	arg2	monoglycosyldiglyceride					1121:1143	monoglycosyldiglyceride	1121:1143	monoglycosyldiglyceride	1121:1143	The polar lipid profile contained the major compounds phosphatidylglycerol, monoglycosyldiglyceride, three unidentified phospholipids, and one unidentified glycolipid.
29458471	9	47	contain	contained	1069:1077	arg2	compounds					1089:1097	the major compounds	1079:1097	the major compounds phosphatidylglycerol, monoglycosyldiglyceride, three unidentified phospholipids, and one unidentified glycolipid	1079:1210	The polar lipid profile contained the major compounds phosphatidylglycerol, monoglycosyldiglyceride, three unidentified phospholipids, and one unidentified glycolipid.
29458471	9	47	contain	contained	1069:1077	arg2	phosphatidylglycerol					1099:1118	phosphatidylglycerol	1099:1118	phosphatidylglycerol	1099:1118	The polar lipid profile contained the major compounds phosphatidylglycerol, monoglycosyldiglyceride, three unidentified phospholipids, and one unidentified glycolipid.
29458471	9	47	contain	contained	1069:1077	arg1	profile					1061:1067	The polar lipid profile	1045:1067	The polar lipid profile	1045:1067	The polar lipid profile contained the major compounds phosphatidylglycerol, monoglycosyldiglyceride, three unidentified phospholipids, and one unidentified glycolipid.
29458471	9	47	contain	contained	1069:1077	arg2	glycolipid					1201:1210	one unidentified glycolipid	1184:1210	one unidentified glycolipid	1184:1210	The polar lipid profile contained the major compounds phosphatidylglycerol, monoglycosyldiglyceride, three unidentified phospholipids, and one unidentified glycolipid.
29458471	2	48	theme	aquarium	296:303	arg1	system					305:310	a marine aquarium system	287:310	a marine aquarium system	287:310	A bright-orange-pigmented, Gram-stain-negative, motile, and rod-shaped bacterium, strain MAA42T, was isolated from a marine sponge of the genus Haliclona, which is in long-time culture in a marine aquarium system at the Justus Liebig University Giessen, Germany.
29458471	5	49	theme	other	652:656	arg1	strains					679:685	all other closely related type strains	648:685	all other closely related type strains	648:685	Sequence similarities to all other closely related type strains were below 97 %.
29458471	2	50	theme	genus	237:241	arg1	Haliclona					243:251	the genus Haliclona	233:251	the genus Haliclona	233:251	A bright-orange-pigmented, Gram-stain-negative, motile, and rod-shaped bacterium, strain MAA42T, was isolated from a marine sponge of the genus Haliclona, which is in long-time culture in a marine aquarium system at the Justus Liebig University Giessen, Germany.
29458471	6	51	theme	DSM	762:764	arg1	22008T					766:771	L. taeanensis DSM 22008T	748:771	L. taeanensis DSM 22008T	748:771	DNA-DNA hybridization of strain MAA42T with L. taeanensis DSM 22008T resulted in values of 4.7 % (reciprocal 17.7 %).
29458471	13	52	theme	Litorimonas	1549:1559	arg1	haliclonae					1561:1570	the name Litorimonas haliclonae	1540:1570	the name Litorimonas haliclonae	1540:1570	Based on the genotypic, chemotaxonomic, and phenotypic analyses, strain MAA42T represents a novel species of the genus Litorimonas, for which the name Litorimonas haliclonae is proposed.
29458471	11	53	theme	meso-diaminopimelic	1329:1347	arg1	acid					1299:1302	The diagnostic diamino acid	1276:1302	The diagnostic diamino acid of the peptidoglycan	1276:1323	The diagnostic diamino acid of the peptidoglycan was meso-diaminopimelic acid.
29458471	11	53	theme	meso-diaminopimelic	1329:1347	arg1	acid					1349:1352	meso-diaminopimelic acid	1329:1352	meso-diaminopimelic acid	1329:1352	The diagnostic diamino acid of the peptidoglycan was meso-diaminopimelic acid.
29458471	6	54	theme	L.	748:749	arg1	22008T					766:771	L. taeanensis DSM 22008T	748:771	L. taeanensis DSM 22008T	748:771	DNA-DNA hybridization of strain MAA42T with L. taeanensis DSM 22008T resulted in values of 4.7 % (reciprocal 17.7 %).
29458471	11	55	theme	peptidoglycan	1311:1323	arg1	acid					1299:1302	The diagnostic diamino acid	1276:1302	The diagnostic diamino acid of the peptidoglycan	1276:1323	The diagnostic diamino acid of the peptidoglycan was meso-diaminopimelic acid.
29458471	11	55	theme	peptidoglycan	1311:1323	arg1	acid					1349:1352	meso-diaminopimelic acid	1329:1352	meso-diaminopimelic acid	1329:1352	The diagnostic diamino acid of the peptidoglycan was meso-diaminopimelic acid.
29458471	2	56	theme	Haliclona	243:251	arg1	sponge					223:228	a marine sponge	214:228	a marine sponge	214:228	A bright-orange-pigmented, Gram-stain-negative, motile, and rod-shaped bacterium, strain MAA42T, was isolated from a marine sponge of the genus Haliclona, which is in long-time culture in a marine aquarium system at the Justus Liebig University Giessen, Germany.
29458471	9	57	theme	unidentified	1188:1199	arg1	compounds					1089:1097	the major compounds	1079:1097	the major compounds phosphatidylglycerol, monoglycosyldiglyceride, three unidentified phospholipids, and one unidentified glycolipid	1079:1210	The polar lipid profile contained the major compounds phosphatidylglycerol, monoglycosyldiglyceride, three unidentified phospholipids, and one unidentified glycolipid.
29458471	9	57	theme	unidentified	1188:1199	arg1	glycolipid					1201:1210	one unidentified glycolipid	1184:1210	one unidentified glycolipid	1184:1210	The polar lipid profile contained the major compounds phosphatidylglycerol, monoglycosyldiglyceride, three unidentified phospholipids, and one unidentified glycolipid.
29458471	6	58	theme	MAA42T	736:741	arg1	hybridization					712:724	DNA-DNA hybridization	704:724	DNA-DNA hybridization of strain MAA42T with L. taeanensis DSM 22008T	704:771	DNA-DNA hybridization of strain MAA42T with L. taeanensis DSM 22008T resulted in values of 4.7 % (reciprocal 17.7 %).
29458471	6	59	theme	17.7 	813:817	arg1	%					818:818	reciprocal 17.7 %	802:818	reciprocal 17.7 %	802:818	DNA-DNA hybridization of strain MAA42T with L. taeanensis DSM 22008T resulted in values of 4.7 % (reciprocal 17.7 %).
29458471	6	59	theme	17.7 	813:817	arg1	%					799:799	4.7 %	795:799	4.7 % (reciprocal 17.7 %)	795:819	DNA-DNA hybridization of strain MAA42T with L. taeanensis DSM 22008T resulted in values of 4.7 % (reciprocal 17.7 %).
29458471	8	60	theme	polyamine	971:979	arg1	pattern					981:987	the polyamine pattern	967:987	the polyamine pattern	967:987	Spermidine was predominant in the polyamine pattern, and ubiquinone Q-10 was the major respiratory quinone.
29458471	3	61	theme	3.5 	455:458	arg1	%					459:459	optimum 3.5 %	447:459	optimum 3.5 %	447:459	The strain grew at 4-34 °C (optimum 28 °C), in the presence of 0.5-9.5 % (w/v) NaCl (optimum 3.5 %) and at pH 4.5-10.0 (optimum pH 7.5).
29458471	3	61	theme	3.5 	455:458	arg1	NaCl					441:444	0.5-9.5 % (w/v) NaCl	425:444	0.5-9.5 % (w/v) NaCl (optimum 3.5 %)	425:460	The strain grew at 4-34 °C (optimum 28 °C), in the presence of 0.5-9.5 % (w/v) NaCl (optimum 3.5 %) and at pH 4.5-10.0 (optimum pH 7.5).
29458471	4	62	theme	16S	532:534	arg1	similarity					555:564	the highest 16S rRNA gene sequence similarity	520:564	the highest 16S rRNA gene sequence similarity (98.1 %)	520:573	Strain MAA42T shared the highest 16S rRNA gene sequence similarity (98.1 %) with the type strain of Litorimonas taeanensis.
29458471	4	62	theme	16S	532:534	arg1	%					572:572	98.1 %	567:572	98.1 %	567:572	Strain MAA42T shared the highest 16S rRNA gene sequence similarity (98.1 %) with the type strain of Litorimonas taeanensis.
29458471	8	63	theme	ubiquinone	994:1003	arg1	quinone					1036:1042	the major respiratory quinone	1014:1042	the major respiratory quinone	1014:1042	Spermidine was predominant in the polyamine pattern, and ubiquinone Q-10 was the major respiratory quinone.
29458471	8	63	theme	ubiquinone	994:1003	arg1	Q-10					1005:1008	ubiquinone Q-10	994:1008	ubiquinone Q-10	994:1008	Spermidine was predominant in the polyamine pattern, and ubiquinone Q-10 was the major respiratory quinone.
29458471	13	64	theme	Litorimonas	1517:1527	arg1	species					1496:1502	a novel species	1488:1502	a novel species	1488:1502	Based on the genotypic, chemotaxonomic, and phenotypic analyses, strain MAA42T represents a novel species of the genus Litorimonas, for which the name Litorimonas haliclonae is proposed.
29458471	1	65	theme	Haliclona	88:96	arg1	sponge					68:73	a marine sponge	59:73	a marine sponge of the genus Haliclona	59:96	nov., isolated from a marine sponge of the genus Haliclona.
29458471	12	66	theme	52.8 mol	1387:1394	arg1	G+C content					1371:1381	The genomic DNA G+C content	1355:1381	The genomic DNA G+C content	1355:1381	The genomic DNA G+C content was 52.8 mol%.
29458471	12	66	theme	52.8 mol	1387:1394	arg1	%					1395:1395	52.8 mol%	1387:1395	52.8 mol%	1387:1395	The genomic DNA G+C content was 52.8 mol%.
29458471	7	67	theme	strain	852:857	arg1	MAA42T					859:864	strain MAA42T	852:864	strain MAA42T	852:864	Major cellular fatty acids of strain MAA42T were C18 : 1ω7c (66.2 %), C18 : 1 2-OH (17.4 %), and C18 : 0 (14.1 %).
29458471	7	68	theme	fatty	837:841	arg1	acids					843:847	Major cellular fatty acids	822:847	Major cellular fatty acids of strain MAA42T	822:864	Major cellular fatty acids of strain MAA42T were C18 : 1ω7c (66.2 %), C18 : 1 2-OH (17.4 %), and C18 : 0 (14.1 %).
29458471	14	69	theme	111178T=LMG	1627:1637	arg1	29765T					1639:1644	=CCM 8709T=CIP 111178T=LMG 29765T	1612:1644	=CCM 8709T=CIP 111178T=LMG 29765T	1612:1644	The type strain is MAA42T (=CCM 8709T=CIP 111178T=LMG 29765T).
29458471	14	69	theme	111178T=LMG	1627:1637	arg1	MAA42T					1604:1609	MAA42T	1604:1609	MAA42T (=CCM 8709T=CIP 111178T=LMG 29765T)	1604:1645	The type strain is MAA42T (=CCM 8709T=CIP 111178T=LMG 29765T).
29458471	8	70	theme	major	1018:1022	arg1	quinone					1036:1042	the major respiratory quinone	1014:1042	the major respiratory quinone	1014:1042	Spermidine was predominant in the polyamine pattern, and ubiquinone Q-10 was the major respiratory quinone.
29458471	8	70	theme	major	1018:1022	arg1	Q-10					1005:1008	ubiquinone Q-10	994:1008	ubiquinone Q-10	994:1008	Spermidine was predominant in the polyamine pattern, and ubiquinone Q-10 was the major respiratory quinone.
29458471	3	71	theme	optimum	482:488	arg1	pH					490:491	optimum pH 7.5	482:495	optimum pH 7.5	482:495	The strain grew at 4-34 °C (optimum 28 °C), in the presence of 0.5-9.5 % (w/v) NaCl (optimum 3.5 %) and at pH 4.5-10.0 (optimum pH 7.5).
29458471	3	71	theme	optimum	482:488	arg1	pH					469:470	pH 4.5-10.0	469:479	pH 4.5-10.0 (optimum pH 7.5)	469:496	The strain grew at 4-34 °C (optimum 28 °C), in the presence of 0.5-9.5 % (w/v) NaCl (optimum 3.5 %) and at pH 4.5-10.0 (optimum pH 7.5).
29458471	7	72	theme	Major	822:826	arg1	acids					843:847	Major cellular fatty acids	822:847	Major cellular fatty acids of strain MAA42T	822:864	Major cellular fatty acids of strain MAA42T were C18 : 1ω7c (66.2 %), C18 : 1 2-OH (17.4 %), and C18 : 0 (14.1 %).
29458471	14	73	theme	=CCM	1612:1615	arg1	29765T					1639:1644	=CCM 8709T=CIP 111178T=LMG 29765T	1612:1644	=CCM 8709T=CIP 111178T=LMG 29765T	1612:1644	The type strain is MAA42T (=CCM 8709T=CIP 111178T=LMG 29765T).
29458471	14	73	theme	=CCM	1612:1615	arg1	MAA42T					1604:1609	MAA42T	1604:1609	MAA42T (=CCM 8709T=CIP 111178T=LMG 29765T)	1604:1645	The type strain is MAA42T (=CCM 8709T=CIP 111178T=LMG 29765T).
29458471	12	74	theme	genomic	1359:1365	arg1	G+C content					1371:1381	The genomic DNA G+C content	1355:1381	The genomic DNA G+C content	1355:1381	The genomic DNA G+C content was 52.8 mol%.
29458471	12	74	theme	genomic	1359:1365	arg1	%					1395:1395	52.8 mol%	1387:1395	52.8 mol%	1387:1395	The genomic DNA G+C content was 52.8 mol%.
29458471	8	75	attach	predominant	952:962	arg2	Spermidine					937:946	Spermidine	937:946	Spermidine	937:946	Spermidine was predominant in the polyamine pattern, and ubiquinone Q-10 was the major respiratory quinone.
29458471	8	75	attach	predominant	952:962	arg1	pattern					981:987	the polyamine pattern	967:987	the polyamine pattern	967:987	Spermidine was predominant in the polyamine pattern, and ubiquinone Q-10 was the major respiratory quinone.
29458471	4	76	theme	gene	541:544	arg1	similarity					555:564	the highest 16S rRNA gene sequence similarity	520:564	the highest 16S rRNA gene sequence similarity (98.1 %)	520:573	Strain MAA42T shared the highest 16S rRNA gene sequence similarity (98.1 %) with the type strain of Litorimonas taeanensis.
29458471	4	76	theme	gene	541:544	arg1	%					572:572	98.1 %	567:572	98.1 %	567:572	Strain MAA42T shared the highest 16S rRNA gene sequence similarity (98.1 %) with the type strain of Litorimonas taeanensis.
29458471	7	77	dep	C18 	919:922	arg1	%					933:933	14.1 %	928:933	14.1 %	928:933	Major cellular fatty acids of strain MAA42T were C18 : 1ω7c (66.2 %), C18 : 1 2-OH (17.4 %), and C18 : 0 (14.1 %).
29458471	7	77	dep	C18 	919:922	arg1	 0					924:925	 0	924:925	 0	924:925	Major cellular fatty acids of strain MAA42T were C18 : 1ω7c (66.2 %), C18 : 1 2-OH (17.4 %), and C18 : 0 (14.1 %).
29458471	11	78	theme	diagnostic	1280:1289	arg1	acid					1299:1302	The diagnostic diamino acid	1276:1302	The diagnostic diamino acid of the peptidoglycan	1276:1323	The diagnostic diamino acid of the peptidoglycan was meso-diaminopimelic acid.
29458471	11	78	theme	diagnostic	1280:1289	arg1	acid					1349:1352	meso-diaminopimelic acid	1329:1352	meso-diaminopimelic acid	1329:1352	The diagnostic diamino acid of the peptidoglycan was meso-diaminopimelic acid.
29458471	4	79	theme	Litorimonas	599:609	arg1	taeanensis					611:620	Litorimonas taeanensis	599:620	Litorimonas taeanensis	599:620	Strain MAA42T shared the highest 16S rRNA gene sequence similarity (98.1 %) with the type strain of Litorimonas taeanensis.
29458471	13	80	theme	strain	1463:1468	arg1	MAA42T					1470:1475	strain MAA42T	1463:1475	strain MAA42T	1463:1475	Based on the genotypic, chemotaxonomic, and phenotypic analyses, strain MAA42T represents a novel species of the genus Litorimonas, for which the name Litorimonas haliclonae is proposed.
29458471	9	81	theme	polar	1049:1053	arg1	profile					1061:1067	The polar lipid profile	1045:1067	The polar lipid profile	1045:1067	The polar lipid profile contained the major compounds phosphatidylglycerol, monoglycosyldiglyceride, three unidentified phospholipids, and one unidentified glycolipid.
29458471	0	82	theme	haliclonae	24:33	arg1	sp					35:36	Litorimonas haliclonae sp	12:36	Litorimonas haliclonae sp	12:36	Proposal of Litorimonas haliclonae sp.
29458471	14	83	theme	type	1589:1592	arg1	MAA42T					1604:1609	MAA42T	1604:1609	MAA42T (=CCM 8709T=CIP 111178T=LMG 29765T)	1604:1645	The type strain is MAA42T (=CCM 8709T=CIP 111178T=LMG 29765T).
29458471	14	83	theme	type	1589:1592	arg1	strain					1594:1599	The type strain	1585:1599	The type strain	1585:1599	The type strain is MAA42T (=CCM 8709T=CIP 111178T=LMG 29765T).
29458471	10	84	theme	minor	1260:1264	arg1	compound					1266:1273	a minor compound	1258:1273	a minor compound	1258:1273	Glucuronopyranosyldiglyceride was present as a minor compound.
29458471	13	85	theme	genotypic	1411:1419	arg1	analyses					1453:1460	the genotypic, chemotaxonomic, and phenotypic analyses	1407:1460	the genotypic, chemotaxonomic, and phenotypic analyses	1407:1460	Based on the genotypic, chemotaxonomic, and phenotypic analyses, strain MAA42T represents a novel species of the genus Litorimonas, for which the name Litorimonas haliclonae is proposed.
29458471	2	86	theme	marine	289:294	arg1	system					305:310	a marine aquarium system	287:310	a marine aquarium system	287:310	A bright-orange-pigmented, Gram-stain-negative, motile, and rod-shaped bacterium, strain MAA42T, was isolated from a marine sponge of the genus Haliclona, which is in long-time culture in a marine aquarium system at the Justus Liebig University Giessen, Germany.
29458471	13	87	theme	chemotaxonomic	1422:1435	arg1	analyses					1453:1460	the genotypic, chemotaxonomic, and phenotypic analyses	1407:1460	the genotypic, chemotaxonomic, and phenotypic analyses	1407:1460	Based on the genotypic, chemotaxonomic, and phenotypic analyses, strain MAA42T represents a novel species of the genus Litorimonas, for which the name Litorimonas haliclonae is proposed.
29458471	13	88	theme	novel	1490:1494	arg1	species					1496:1502	a novel species	1488:1502	a novel species	1488:1502	Based on the genotypic, chemotaxonomic, and phenotypic analyses, strain MAA42T represents a novel species of the genus Litorimonas, for which the name Litorimonas haliclonae is proposed.
29458471	2	89	from	Germany	353:359	arg1	culture					276:282	long-time culture	266:282	long-time culture in a marine aquarium system at the Justus Liebig University Giessen, Germany	266:359	A bright-orange-pigmented, Gram-stain-negative, motile, and rod-shaped bacterium, strain MAA42T, was isolated from a marine sponge of the genus Haliclona, which is in long-time culture in a marine aquarium system at the Justus Liebig University Giessen, Germany.
29458471	2	90	theme	bright-orange-pigmented	101:123	arg1	strain					181:186	strain MAA42T	181:193	strain MAA42T	181:193	A bright-orange-pigmented, Gram-stain-negative, motile, and rod-shaped bacterium, strain MAA42T, was isolated from a marine sponge of the genus Haliclona, which is in long-time culture in a marine aquarium system at the Justus Liebig University Giessen, Germany.
29458471	2	90	theme	bright-orange-pigmented	101:123	arg1	bacterium					170:178	A bright-orange-pigmented, Gram-stain-negative, motile, and rod-shaped bacterium	99:178	A bright-orange-pigmented, Gram-stain-negative, motile, and rod-shaped bacterium	99:178	A bright-orange-pigmented, Gram-stain-negative, motile, and rod-shaped bacterium, strain MAA42T, was isolated from a marine sponge of the genus Haliclona, which is in long-time culture in a marine aquarium system at the Justus Liebig University Giessen, Germany.
29458471	3	91	theme	w/v	436:438	arg1	%					459:459	optimum 3.5 %	447:459	optimum 3.5 %	447:459	The strain grew at 4-34 °C (optimum 28 °C), in the presence of 0.5-9.5 % (w/v) NaCl (optimum 3.5 %) and at pH 4.5-10.0 (optimum pH 7.5).
29458471	3	91	theme	w/v	436:438	arg1	NaCl					441:444	0.5-9.5 % (w/v) NaCl	425:444	0.5-9.5 % (w/v) NaCl (optimum 3.5 %)	425:460	The strain grew at 4-34 °C (optimum 28 °C), in the presence of 0.5-9.5 % (w/v) NaCl (optimum 3.5 %) and at pH 4.5-10.0 (optimum pH 7.5).
29458471	9	92	theme	unidentified	1152:1163	arg1	phospholipids					1165:1177	three unidentified phospholipids	1146:1177	three unidentified phospholipids	1146:1177	The polar lipid profile contained the major compounds phosphatidylglycerol, monoglycosyldiglyceride, three unidentified phospholipids, and one unidentified glycolipid.
30538182	10	0	from	pathways	1973:1980	arg1	mobilization					1989:2000	the mobilization	1985:2000	the mobilization of carbohydrates for fungal cell wall biosynthesis	1985:2051	This report elucidates the cooperation between the HOG and PKA pathways in the mobilization of carbohydrates for fungal cell wall biosynthesis.
30538182	7	1	theme	immunocompromised	1527:1543	arg1	patients					1545:1552	immunocompromised patients	1527:1552	immunocompromised patients	1527:1552	This work reveals the communication between the HOG and PKA pathways for carbohydrate mobilization for cell wall construction.IMPORTANCEAspergillus fumigatus is an opportunistic human pathogen causing allergic reactions or systemic infections such as invasive pulmonary aspergillosis, especially in immunocompromised patients.
30538182	11	2	from	defects	2119:2125	arg1	structure					2134:2142	the structure	2130:2142	the structure of the fungal cell wall	2130:2166	We suggest that the reduced mobilization of simple sugars causes defects in the structure of the fungal cell wall.
30538182	8	3	theme	fungal	1742:1747	arg1	wall					1754:1757	the fungal cell wall	1738:1757	the fungal cell wall called pathogen-associated molecular patterns (PAMPs)	1738:1811	The fungal cell wall is the main component responsible for recognition by the immune system, due to the specific composition of polysaccharide carbohydrates exposed on the surface of the fungal cell wall called pathogen-associated molecular patterns (PAMPs).
30538182	3	4	theme	high-osmolarity	562:576	arg1	SakA					608:611	SakA	608:611	SakA	608:611	Here we show that this process is dependent on cAMP-dependent protein kinase A (PKA) activity and regulated by the high-osmolarity glycerol response (HOG) MAPKs SakA and MpkC.
30538182	3	4	theme	high-osmolarity	562:576	arg1	MAPKs					602:606	the high-osmolarity glycerol response (HOG) MAPKs	558:606	the high-osmolarity glycerol response (HOG) MAPKs SakA and MpkC	558:620	Here we show that this process is dependent on cAMP-dependent protein kinase A (PKA) activity and regulated by the high-osmolarity glycerol response (HOG) MAPKs SakA and MpkC.
30538182	3	4	theme	high-osmolarity	562:576	arg1	MpkC					617:620	MpkC	617:620	MpkC	617:620	Here we show that this process is dependent on cAMP-dependent protein kinase A (PKA) activity and regulated by the high-osmolarity glycerol response (HOG) MAPKs SakA and MpkC.
30538182	6	5	theme	carbohydrate	1201:1212	arg1	mobilization					1214:1225	carbohydrate mobilization	1201:1225	carbohydrate mobilization	1201:1225	Carbohydrate mobilization is controlled by SakA interaction with PkaC1 and PkaR, suggesting a putative mechanism where the PkaR regulatory subunit leaves the complex and releases the SakA-PkaC1 complex for activation of enzymes involved in carbohydrate mobilization.
30538182	10	6	theme	cell	2030:2033	arg1	biosynthesis					2040:2051	fungal cell wall biosynthesis	2023:2051	fungal cell wall biosynthesis	2023:2051	This report elucidates the cooperation between the HOG and PKA pathways in the mobilization of carbohydrates for fungal cell wall biosynthesis.
30538182	11	7	theme	sugars	2105:2110	arg1	mobilization					2082:2093	the reduced mobilization	2070:2093	the reduced mobilization of simple sugars	2070:2110	We suggest that the reduced mobilization of simple sugars causes defects in the structure of the fungal cell wall.
30538182	3	8	dep	MAPKs	602:606	arg1	SakA					608:611	SakA	608:611	SakA	608:611	Here we show that this process is dependent on cAMP-dependent protein kinase A (PKA) activity and regulated by the high-osmolarity glycerol response (HOG) MAPKs SakA and MpkC.
30538182	3	8	dep	MAPKs	602:606	arg1	MAPKs					602:606	the high-osmolarity glycerol response (HOG) MAPKs	558:606	the high-osmolarity glycerol response (HOG) MAPKs SakA and MpkC	558:620	Here we show that this process is dependent on cAMP-dependent protein kinase A (PKA) activity and regulated by the high-osmolarity glycerol response (HOG) MAPKs SakA and MpkC.
30538182	3	8	dep	MAPKs	602:606	arg1	MpkC					617:620	MpkC	617:620	MpkC	617:620	Here we show that this process is dependent on cAMP-dependent protein kinase A (PKA) activity and regulated by the high-osmolarity glycerol response (HOG) MAPKs SakA and MpkC.
30538182	4	9	theme	trehalose	698:706	arg1	accumulation/degradation					670:693	normal accumulation/degradation	663:693	normal accumulation/degradation of trehalose and glycogen	663:719	These protein kinases are necessary for normal accumulation/degradation of trehalose and glycogen, and the lack of these genes reduces glucose uptake and glycogen synthesis.
30538182	2	10	theme	cell	344:347	arg1	stress					354:359	cell wall stress	344:359	cell wall stress	344:359	Upon cell wall stress, cell wall-related sugars need to be synthesized from carbohydrate storage compounds.
30538182	4	11	theme	glycogen	777:784	arg1	synthesis					786:794	glycogen synthesis	777:794	glycogen synthesis	777:794	These protein kinases are necessary for normal accumulation/degradation of trehalose and glycogen, and the lack of these genes reduces glucose uptake and glycogen synthesis.
30538182	11	12	theme	cell	2158:2161	arg1	wall					2163:2166	the fungal cell wall	2147:2166	the fungal cell wall	2147:2166	We suggest that the reduced mobilization of simple sugars causes defects in the structure of the fungal cell wall.
30538182	7	13	theme	invasive	1479:1486	arg1	aspergillosis					1498:1510	invasive pulmonary aspergillosis	1479:1510	invasive pulmonary aspergillosis	1479:1510	This work reveals the communication between the HOG and PKA pathways for carbohydrate mobilization for cell wall construction.IMPORTANCEAspergillus fumigatus is an opportunistic human pathogen causing allergic reactions or systemic infections such as invasive pulmonary aspergillosis, especially in immunocompromised patients.
30538182	3	14	theme	response	587:594	arg1	SakA					608:611	SakA	608:611	SakA	608:611	Here we show that this process is dependent on cAMP-dependent protein kinase A (PKA) activity and regulated by the high-osmolarity glycerol response (HOG) MAPKs SakA and MpkC.
30538182	3	14	theme	response	587:594	arg1	MAPKs					602:606	the high-osmolarity glycerol response (HOG) MAPKs	558:606	the high-osmolarity glycerol response (HOG) MAPKs SakA and MpkC	558:620	Here we show that this process is dependent on cAMP-dependent protein kinase A (PKA) activity and regulated by the high-osmolarity glycerol response (HOG) MAPKs SakA and MpkC.
30538182	3	14	theme	response	587:594	arg1	MpkC					617:620	MpkC	617:620	MpkC	617:620	Here we show that this process is dependent on cAMP-dependent protein kinase A (PKA) activity and regulated by the high-osmolarity glycerol response (HOG) MAPKs SakA and MpkC.
30538182	9	15	theme	Key	1814:1816	arg1	target					1874:1879	a good target	1867:1879	a good target for fungal drug development	1867:1907	Key enzymes in the fungal cell wall biosynthesis are a good target for fungal drug development.
30538182	9	15	theme	Key	1814:1816	arg1	enzymes					1818:1824	Key enzymes	1814:1824	Key enzymes in the fungal cell wall biosynthesis	1814:1861	Key enzymes in the fungal cell wall biosynthesis are a good target for fungal drug development.
30538182	7	16	theme	opportunistic	1392:1404	arg1	mobilization					1314:1325	carbohydrate mobilization	1301:1325	carbohydrate mobilization for cell wall construction.IMPORTANCEAspergillus fumigatus	1301:1384	This work reveals the communication between the HOG and PKA pathways for carbohydrate mobilization for cell wall construction.IMPORTANCEAspergillus fumigatus is an opportunistic human pathogen causing allergic reactions or systemic infections such as invasive pulmonary aspergillosis, especially in immunocompromised patients.
30538182	7	16	theme	opportunistic	1392:1404	arg1	pathogen					1412:1419	an opportunistic human pathogen	1389:1419	an opportunistic human pathogen causing allergic reactions or systemic infections such as invasive pulmonary aspergillosis	1389:1510	This work reveals the communication between the HOG and PKA pathways for carbohydrate mobilization for cell wall construction.IMPORTANCEAspergillus fumigatus is an opportunistic human pathogen causing allergic reactions or systemic infections such as invasive pulmonary aspergillosis, especially in immunocompromised patients.
30538182	1	17	theme	normal	244:249	arg1	morphology					251:260	the normal morphology	240:260	the normal morphology of the cell wall	240:277	Aspergillus fumigatus mitogen-activated protein kinases (MAPKs) are involved in maintaining the normal morphology of the cell wall and providing resistance against cell wall-damaging agents.
30538182	6	18	theme	Carbohydrate	961:972	arg1	mobilization					974:985	Carbohydrate mobilization	961:985	Carbohydrate mobilization	961:985	Carbohydrate mobilization is controlled by SakA interaction with PkaC1 and PkaR, suggesting a putative mechanism where the PkaR regulatory subunit leaves the complex and releases the SakA-PkaC1 complex for activation of enzymes involved in carbohydrate mobilization.
30538182	8	19	theme	fungal	1559:1564	arg1	component					1588:1596	the main component	1579:1596	the main component responsible for recognition by the immune system, due to the specific composition of polysaccharide carbohydrates exposed on the surface of the fungal cell wall called pathogen-associated molecular patterns (PAMPs)	1579:1811	The fungal cell wall is the main component responsible for recognition by the immune system, due to the specific composition of polysaccharide carbohydrates exposed on the surface of the fungal cell wall called pathogen-associated molecular patterns (PAMPs).
30538182	8	19	theme	fungal	1559:1564	arg1	wall					1571:1574	The fungal cell wall	1555:1574	The fungal cell wall	1555:1574	The fungal cell wall is the main component responsible for recognition by the immune system, due to the specific composition of polysaccharide carbohydrates exposed on the surface of the fungal cell wall called pathogen-associated molecular patterns (PAMPs).
30538182	7	20	theme	systemic	1451:1458	arg1	aspergillosis					1498:1510	invasive pulmonary aspergillosis	1479:1510	invasive pulmonary aspergillosis	1479:1510	This work reveals the communication between the HOG and PKA pathways for carbohydrate mobilization for cell wall construction.IMPORTANCEAspergillus fumigatus is an opportunistic human pathogen causing allergic reactions or systemic infections such as invasive pulmonary aspergillosis, especially in immunocompromised patients.
30538182	7	20	theme	systemic	1451:1458	arg1	infections					1460:1469	systemic infections	1451:1469	systemic infections	1451:1469	This work reveals the communication between the HOG and PKA pathways for carbohydrate mobilization for cell wall construction.IMPORTANCEAspergillus fumigatus is an opportunistic human pathogen causing allergic reactions or systemic infections such as invasive pulmonary aspergillosis, especially in immunocompromised patients.
30538182	9	21	theme	fungal	1833:1838	arg1	biosynthesis					1850:1861	the fungal cell wall biosynthesis	1829:1861	the fungal cell wall biosynthesis	1829:1861	Key enzymes in the fungal cell wall biosynthesis are a good target for fungal drug development.
30538182	5	22	theme	cell	950:953	arg1	wall					955:958	the cell wall	946:958	the cell wall	946:958	Alterations in glycogen synthesis were observed for the sakA and mpkC deletion mutants, which also displayed alterations in carbohydrate exposure on the cell wall.
30538182	9	23	theme	wall	1845:1848	arg1	biosynthesis					1850:1861	the fungal cell wall biosynthesis	1829:1861	the fungal cell wall biosynthesis	1829:1861	Key enzymes in the fungal cell wall biosynthesis are a good target for fungal drug development.
30538182	4	24	theme	glycogen	712:719	arg1	accumulation/degradation					670:693	normal accumulation/degradation	663:693	normal accumulation/degradation of trehalose and glycogen	663:719	These protein kinases are necessary for normal accumulation/degradation of trehalose and glycogen, and the lack of these genes reduces glucose uptake and glycogen synthesis.
30538182	7	25	theme	HOG	1276:1278	arg1	pathways					1288:1295	the HOG and PKA pathways	1272:1295	pathways	1288:1295	This work reveals the communication between the HOG and PKA pathways for carbohydrate mobilization for cell wall construction.IMPORTANCEAspergillus fumigatus is an opportunistic human pathogen causing allergic reactions or systemic infections such as invasive pulmonary aspergillosis, especially in immunocompromised patients.
30538182	11	26	theme	reduced	2074:2080	arg1	mobilization					2082:2093	the reduced mobilization	2070:2093	the reduced mobilization of simple sugars	2070:2110	We suggest that the reduced mobilization of simple sugars causes defects in the structure of the fungal cell wall.
30538182	1	27	theme	protein	188:194	arg1	MAPKs					205:209	MAPKs	205:209	MAPKs	205:209	Aspergillus fumigatus mitogen-activated protein kinases (MAPKs) are involved in maintaining the normal morphology of the cell wall and providing resistance against cell wall-damaging agents.
30538182	1	27	theme	protein	188:194	arg1	kinases					196:202	Aspergillus fumigatus mitogen-activated protein kinases	148:202	Aspergillus fumigatus mitogen-activated protein kinases (MAPKs)	148:210	Aspergillus fumigatus mitogen-activated protein kinases (MAPKs) are involved in maintaining the normal morphology of the cell wall and providing resistance against cell wall-damaging agents.
30538182	0	28	theme	Cell	86:89	arg1	Mobilization					109:120	Cell Wall Carbohydrate Mobilization	86:120	Cell Wall Carbohydrate Mobilization	86:120	Protein Kinase A and High-Osmolarity Glycerol Response Pathways Cooperatively Control Cell Wall Carbohydrate Mobilization in Aspergillus fumigatus.
30538182	5	29	from	Alterations	797:807	arg1	synthesis					821:829	glycogen synthesis	812:829	glycogen synthesis	812:829	Alterations in glycogen synthesis were observed for the sakA and mpkC deletion mutants, which also displayed alterations in carbohydrate exposure on the cell wall.
30538182	5	30	theme	carbohydrate	921:932	arg1	exposure					934:941	carbohydrate exposure	921:941	carbohydrate exposure	921:941	Alterations in glycogen synthesis were observed for the sakA and mpkC deletion mutants, which also displayed alterations in carbohydrate exposure on the cell wall.
30538182	9	31	from	enzymes	1818:1824	arg1	biosynthesis					1850:1861	the fungal cell wall biosynthesis	1829:1861	the fungal cell wall biosynthesis	1829:1861	Key enzymes in the fungal cell wall biosynthesis are a good target for fungal drug development.
30538182	9	32	theme	good	1869:1872	arg1	target					1874:1879	a good target	1867:1879	a good target for fungal drug development	1867:1907	Key enzymes in the fungal cell wall biosynthesis are a good target for fungal drug development.
30538182	9	32	theme	good	1869:1872	arg1	enzymes					1818:1824	Key enzymes	1814:1824	Key enzymes in the fungal cell wall biosynthesis	1814:1861	Key enzymes in the fungal cell wall biosynthesis are a good target for fungal drug development.
30538182	12	33	theme	stress	2381:2386	arg1	response					2388:2395	the osmotic stress response	2369:2395	the osmotic stress response	2369:2395	In summary, we propose that SakA is important for PKA activity, therefore regulating the availability and mobilization of monosaccharides for fungal cell wall biosynthesis during cell wall damage and the osmotic stress response.
30538182	1	34	dep	Aspergillus	148:158	arg1	fumigatus					160:168	fumigatus	160:168	fumigatus	160:168	Aspergillus fumigatus mitogen-activated protein kinases (MAPKs) are involved in maintaining the normal morphology of the cell wall and providing resistance against cell wall-damaging agents.
30538182	0	35	theme	Carbohydrate	96:107	arg1	Mobilization					109:120	Cell Wall Carbohydrate Mobilization	86:120	Cell Wall Carbohydrate Mobilization	86:120	Protein Kinase A and High-Osmolarity Glycerol Response Pathways Cooperatively Control Cell Wall Carbohydrate Mobilization in Aspergillus fumigatus.
30538182	12	36	theme	cell	2348:2351	arg1	damage					2358:2363	cell wall damage	2348:2363	cell wall damage	2348:2363	In summary, we propose that SakA is important for PKA activity, therefore regulating the availability and mobilization of monosaccharides for fungal cell wall biosynthesis during cell wall damage and the osmotic stress response.
30538182	2	37	theme	storage	428:434	arg1	compounds					436:444	carbohydrate storage compounds	415:444	carbohydrate storage compounds	415:444	Upon cell wall stress, cell wall-related sugars need to be synthesized from carbohydrate storage compounds.
30538182	9	38	theme	drug	1892:1895	arg1	development					1897:1907	fungal drug development	1885:1907	fungal drug development	1885:1907	Key enzymes in the fungal cell wall biosynthesis are a good target for fungal drug development.
30538182	8	39	theme	immune	1633:1638	arg1	system					1640:1645	the immune system	1629:1645	the immune system	1629:1645	The fungal cell wall is the main component responsible for recognition by the immune system, due to the specific composition of polysaccharide carbohydrates exposed on the surface of the fungal cell wall called pathogen-associated molecular patterns (PAMPs).
30538182	12	40	theme	monosaccharides	2291:2305	arg1	availability					2258:2269	availability	2258:2269	availability	2258:2269	In summary, we propose that SakA is important for PKA activity, therefore regulating the availability and mobilization of monosaccharides for fungal cell wall biosynthesis during cell wall damage and the osmotic stress response.
30538182	12	40	theme	monosaccharides	2291:2305	arg1	mobilization					2275:2286	mobilization	2275:2286	mobilization	2275:2286	In summary, we propose that SakA is important for PKA activity, therefore regulating the availability and mobilization of monosaccharides for fungal cell wall biosynthesis during cell wall damage and the osmotic stress response.
30538182	10	41	theme	wall	2035:2038	arg1	biosynthesis					2040:2051	fungal cell wall biosynthesis	2023:2051	fungal cell wall biosynthesis	2023:2051	This report elucidates the cooperation between the HOG and PKA pathways in the mobilization of carbohydrates for fungal cell wall biosynthesis.
30538182	0	42	theme	Protein	0:6	arg1	A					15:15	Protein Kinase A	0:15	Protein Kinase A	0:15	Protein Kinase A and High-Osmolarity Glycerol Response Pathways Cooperatively Control Cell Wall Carbohydrate Mobilization in Aspergillus fumigatus.
30538182	6	43	theme	PkaR	1084:1087	arg1	subunit					1100:1106	the PkaR regulatory subunit	1080:1106	the PkaR regulatory subunit	1080:1106	Carbohydrate mobilization is controlled by SakA interaction with PkaC1 and PkaR, suggesting a putative mechanism where the PkaR regulatory subunit leaves the complex and releases the SakA-PkaC1 complex for activation of enzymes involved in carbohydrate mobilization.
30538182	7	44	dep	communication	1250:1262	arg1	mobilization					1314:1325	carbohydrate mobilization	1301:1325	carbohydrate mobilization for cell wall construction.IMPORTANCEAspergillus fumigatus	1301:1384	This work reveals the communication between the HOG and PKA pathways for carbohydrate mobilization for cell wall construction.IMPORTANCEAspergillus fumigatus is an opportunistic human pathogen causing allergic reactions or systemic infections such as invasive pulmonary aspergillosis, especially in immunocompromised patients.
30538182	7	44	dep	communication	1250:1262	arg1	pathogen					1412:1419	an opportunistic human pathogen	1389:1419	an opportunistic human pathogen causing allergic reactions or systemic infections such as invasive pulmonary aspergillosis	1389:1510	This work reveals the communication between the HOG and PKA pathways for carbohydrate mobilization for cell wall construction.IMPORTANCEAspergillus fumigatus is an opportunistic human pathogen causing allergic reactions or systemic infections such as invasive pulmonary aspergillosis, especially in immunocompromised patients.
30538182	12	45	theme	fungal	2311:2316	arg1	biosynthesis					2328:2339	fungal cell wall biosynthesis	2311:2339	fungal cell wall biosynthesis	2311:2339	In summary, we propose that SakA is important for PKA activity, therefore regulating the availability and mobilization of monosaccharides for fungal cell wall biosynthesis during cell wall damage and the osmotic stress response.
30538182	1	46	theme	wall-damaging	317:329	arg1	agents					331:336	cell wall-damaging agents	312:336	cell wall-damaging agents	312:336	Aspergillus fumigatus mitogen-activated protein kinases (MAPKs) are involved in maintaining the normal morphology of the cell wall and providing resistance against cell wall-damaging agents.
30538182	10	47	theme	HOG	1961:1963	arg1	pathways					1973:1980	the HOG and PKA pathways	1957:1980	pathways	1973:1980	This report elucidates the cooperation between the HOG and PKA pathways in the mobilization of carbohydrates for fungal cell wall biosynthesis.
30538182	0	48	theme	High-Osmolarity	21:35	arg1	Pathways					55:62	High-Osmolarity Glycerol Response Pathways	21:62	High-Osmolarity Glycerol Response Pathways	21:62	Protein Kinase A and High-Osmolarity Glycerol Response Pathways Cooperatively Control Cell Wall Carbohydrate Mobilization in Aspergillus fumigatus.
30538182	7	49	theme	construction.IMPORTANCEAspergillus	1341:1374	arg1	wall					1336:1339	cell wall	1331:1339	cell wall construction.IMPORTANCEAspergillus fumigatus	1331:1384	This work reveals the communication between the HOG and PKA pathways for carbohydrate mobilization for cell wall construction.IMPORTANCEAspergillus fumigatus is an opportunistic human pathogen causing allergic reactions or systemic infections such as invasive pulmonary aspergillosis, especially in immunocompromised patients.
30538182	8	50	theme	specific	1659:1666	arg1	composition					1668:1678	the specific composition	1655:1678	the specific composition of polysaccharide carbohydrates exposed on the surface of the fungal cell wall called pathogen-associated molecular patterns (PAMPs)	1655:1811	The fungal cell wall is the main component responsible for recognition by the immune system, due to the specific composition of polysaccharide carbohydrates exposed on the surface of the fungal cell wall called pathogen-associated molecular patterns (PAMPs).
30538182	3	51	theme	A	524:524	arg1	activity					532:539	cAMP-dependent protein kinase A (PKA) activity	494:539	cAMP-dependent protein kinase A (PKA) activity	494:539	Here we show that this process is dependent on cAMP-dependent protein kinase A (PKA) activity and regulated by the high-osmolarity glycerol response (HOG) MAPKs SakA and MpkC.
30538182	0	52	theme	Response	46:53	arg1	Pathways					55:62	High-Osmolarity Glycerol Response Pathways	21:62	High-Osmolarity Glycerol Response Pathways	21:62	Protein Kinase A and High-Osmolarity Glycerol Response Pathways Cooperatively Control Cell Wall Carbohydrate Mobilization in Aspergillus fumigatus.
30538182	12	53	theme	osmotic	2373:2379	arg1	response					2388:2395	the osmotic stress response	2369:2395	the osmotic stress response	2369:2395	In summary, we propose that SakA is important for PKA activity, therefore regulating the availability and mobilization of monosaccharides for fungal cell wall biosynthesis during cell wall damage and the osmotic stress response.
30538182	7	54	theme	cell	1331:1334	arg1	wall					1336:1339	cell wall	1331:1339	cell wall construction.IMPORTANCEAspergillus fumigatus	1331:1384	This work reveals the communication between the HOG and PKA pathways for carbohydrate mobilization for cell wall construction.IMPORTANCEAspergillus fumigatus is an opportunistic human pathogen causing allergic reactions or systemic infections such as invasive pulmonary aspergillosis, especially in immunocompromised patients.
30538182	3	55	theme	protein	509:515	arg1	A					524:524	cAMP-dependent protein kinase A	494:524	cAMP-dependent protein kinase A (PKA) activity	494:539	Here we show that this process is dependent on cAMP-dependent protein kinase A (PKA) activity and regulated by the high-osmolarity glycerol response (HOG) MAPKs SakA and MpkC.
30538182	3	55	theme	protein	509:515	arg1	PKA					527:529	PKA	527:529	PKA	527:529	Here we show that this process is dependent on cAMP-dependent protein kinase A (PKA) activity and regulated by the high-osmolarity glycerol response (HOG) MAPKs SakA and MpkC.
30538182	8	56	theme	cell	1749:1752	arg1	wall					1754:1757	the fungal cell wall	1738:1757	the fungal cell wall called pathogen-associated molecular patterns (PAMPs)	1738:1811	The fungal cell wall is the main component responsible for recognition by the immune system, due to the specific composition of polysaccharide carbohydrates exposed on the surface of the fungal cell wall called pathogen-associated molecular patterns (PAMPs).
30538182	7	57	dep	construction.IMPORTANCEAspergillus	1341:1374	arg1	fumigatus					1376:1384	fumigatus	1376:1384	fumigatus	1376:1384	This work reveals the communication between the HOG and PKA pathways for carbohydrate mobilization for cell wall construction.IMPORTANCEAspergillus fumigatus is an opportunistic human pathogen causing allergic reactions or systemic infections such as invasive pulmonary aspergillosis, especially in immunocompromised patients.
30538182	8	58	theme	carbohydrates	1698:1710	arg1	composition					1668:1678	the specific composition	1655:1678	the specific composition of polysaccharide carbohydrates exposed on the surface of the fungal cell wall called pathogen-associated molecular patterns (PAMPs)	1655:1811	The fungal cell wall is the main component responsible for recognition by the immune system, due to the specific composition of polysaccharide carbohydrates exposed on the surface of the fungal cell wall called pathogen-associated molecular patterns (PAMPs).
30538182	12	59	theme	wall	2323:2326	arg1	biosynthesis					2328:2339	fungal cell wall biosynthesis	2311:2339	fungal cell wall biosynthesis	2311:2339	In summary, we propose that SakA is important for PKA activity, therefore regulating the availability and mobilization of monosaccharides for fungal cell wall biosynthesis during cell wall damage and the osmotic stress response.
30538182	6	60	theme	putative	1055:1062	arg1	mechanism					1064:1072	a putative mechanism	1053:1072	a putative mechanism where the PkaR regulatory subunit leaves the complex and releases the SakA-PkaC1 complex for activation of enzymes involved in carbohydrate mobilization	1053:1225	Carbohydrate mobilization is controlled by SakA interaction with PkaC1 and PkaR, suggesting a putative mechanism where the PkaR regulatory subunit leaves the complex and releases the SakA-PkaC1 complex for activation of enzymes involved in carbohydrate mobilization.
30538182	5	61	dep	sakA	853:856	arg1	the					849:851	the	849:851	the	849:851	Alterations in glycogen synthesis were observed for the sakA and mpkC deletion mutants, which also displayed alterations in carbohydrate exposure on the cell wall.
30538182	10	62	theme	carbohydrates	2005:2017	arg1	mobilization					1989:2000	the mobilization	1985:2000	the mobilization of carbohydrates for fungal cell wall biosynthesis	1985:2051	This report elucidates the cooperation between the HOG and PKA pathways in the mobilization of carbohydrates for fungal cell wall biosynthesis.
30538182	8	63	theme	molecular	1786:1794	arg1	patterns					1796:1803	pathogen-associated molecular patterns	1766:1803	pathogen-associated molecular patterns (PAMPs)	1766:1811	The fungal cell wall is the main component responsible for recognition by the immune system, due to the specific composition of polysaccharide carbohydrates exposed on the surface of the fungal cell wall called pathogen-associated molecular patterns (PAMPs).
30538182	8	63	theme	molecular	1786:1794	arg1	PAMPs					1806:1810	PAMPs	1806:1810	PAMPs	1806:1810	The fungal cell wall is the main component responsible for recognition by the immune system, due to the specific composition of polysaccharide carbohydrates exposed on the surface of the fungal cell wall called pathogen-associated molecular patterns (PAMPs).
30538182	1	64	theme	wall	274:277	arg1	morphology					251:260	the normal morphology	240:260	the normal morphology of the cell wall	240:277	Aspergillus fumigatus mitogen-activated protein kinases (MAPKs) are involved in maintaining the normal morphology of the cell wall and providing resistance against cell wall-damaging agents.
30538182	7	65	theme	PKA	1284:1286	arg1	pathways					1288:1295	the HOG and PKA pathways	1272:1295	pathways	1288:1295	This work reveals the communication between the HOG and PKA pathways for carbohydrate mobilization for cell wall construction.IMPORTANCEAspergillus fumigatus is an opportunistic human pathogen causing allergic reactions or systemic infections such as invasive pulmonary aspergillosis, especially in immunocompromised patients.
30538182	2	66	theme	wall-related	367:378	arg1	sugars					380:385	cell wall-related sugars	362:385	cell wall-related sugars	362:385	Upon cell wall stress, cell wall-related sugars need to be synthesized from carbohydrate storage compounds.
30538182	11	67	theme	simple	2098:2103	arg1	sugars					2105:2110	simple sugars	2098:2110	simple sugars	2098:2110	We suggest that the reduced mobilization of simple sugars causes defects in the structure of the fungal cell wall.
30538182	12	68	theme	PKA	2219:2221	arg1	activity					2223:2230	PKA activity	2219:2230	PKA activity	2219:2230	In summary, we propose that SakA is important for PKA activity, therefore regulating the availability and mobilization of monosaccharides for fungal cell wall biosynthesis during cell wall damage and the osmotic stress response.
30538182	4	69	theme	normal	663:668	arg1	accumulation/degradation					670:693	normal accumulation/degradation	663:693	normal accumulation/degradation of trehalose and glycogen	663:719	These protein kinases are necessary for normal accumulation/degradation of trehalose and glycogen, and the lack of these genes reduces glucose uptake and glycogen synthesis.
30538182	10	70	theme	fungal	2023:2028	arg1	biosynthesis					2040:2051	fungal cell wall biosynthesis	2023:2051	fungal cell wall biosynthesis	2023:2051	This report elucidates the cooperation between the HOG and PKA pathways in the mobilization of carbohydrates for fungal cell wall biosynthesis.
30538182	2	71	theme	wall	349:352	arg1	stress					354:359	cell wall stress	344:359	cell wall stress	344:359	Upon cell wall stress, cell wall-related sugars need to be synthesized from carbohydrate storage compounds.
30538182	6	72	theme	SakA	1004:1007	arg1	interaction					1009:1019	SakA interaction	1004:1019	SakA interaction with PkaC1 and PkaR	1004:1039	Carbohydrate mobilization is controlled by SakA interaction with PkaC1 and PkaR, suggesting a putative mechanism where the PkaR regulatory subunit leaves the complex and releases the SakA-PkaC1 complex for activation of enzymes involved in carbohydrate mobilization.
30538182	6	73	theme	enzymes	1181:1187	arg1	activation					1167:1176	activation	1167:1176	activation of enzymes involved in carbohydrate mobilization	1167:1225	Carbohydrate mobilization is controlled by SakA interaction with PkaC1 and PkaR, suggesting a putative mechanism where the PkaR regulatory subunit leaves the complex and releases the SakA-PkaC1 complex for activation of enzymes involved in carbohydrate mobilization.
30538182	3	74	theme	glycerol	578:585	arg1	SakA					608:611	SakA	608:611	SakA	608:611	Here we show that this process is dependent on cAMP-dependent protein kinase A (PKA) activity and regulated by the high-osmolarity glycerol response (HOG) MAPKs SakA and MpkC.
30538182	3	74	theme	glycerol	578:585	arg1	MAPKs					602:606	the high-osmolarity glycerol response (HOG) MAPKs	558:606	the high-osmolarity glycerol response (HOG) MAPKs SakA and MpkC	558:620	Here we show that this process is dependent on cAMP-dependent protein kinase A (PKA) activity and regulated by the high-osmolarity glycerol response (HOG) MAPKs SakA and MpkC.
30538182	3	74	theme	glycerol	578:585	arg1	MpkC					617:620	MpkC	617:620	MpkC	617:620	Here we show that this process is dependent on cAMP-dependent protein kinase A (PKA) activity and regulated by the high-osmolarity glycerol response (HOG) MAPKs SakA and MpkC.
30538182	3	75	theme	HOG	597:599	arg1	SakA					608:611	SakA	608:611	SakA	608:611	Here we show that this process is dependent on cAMP-dependent protein kinase A (PKA) activity and regulated by the high-osmolarity glycerol response (HOG) MAPKs SakA and MpkC.
30538182	3	75	theme	HOG	597:599	arg1	MAPKs					602:606	the high-osmolarity glycerol response (HOG) MAPKs	558:606	the high-osmolarity glycerol response (HOG) MAPKs SakA and MpkC	558:620	Here we show that this process is dependent on cAMP-dependent protein kinase A (PKA) activity and regulated by the high-osmolarity glycerol response (HOG) MAPKs SakA and MpkC.
30538182	3	75	theme	HOG	597:599	arg1	MpkC					617:620	MpkC	617:620	MpkC	617:620	Here we show that this process is dependent on cAMP-dependent protein kinase A (PKA) activity and regulated by the high-osmolarity glycerol response (HOG) MAPKs SakA and MpkC.
30538182	11	76	theme	wall	2163:2166	arg1	structure					2134:2142	the structure	2130:2142	the structure of the fungal cell wall	2130:2166	We suggest that the reduced mobilization of simple sugars causes defects in the structure of the fungal cell wall.
30538182	7	77	theme	pulmonary	1488:1496	arg1	aspergillosis					1498:1510	invasive pulmonary aspergillosis	1479:1510	invasive pulmonary aspergillosis	1479:1510	This work reveals the communication between the HOG and PKA pathways for carbohydrate mobilization for cell wall construction.IMPORTANCEAspergillus fumigatus is an opportunistic human pathogen causing allergic reactions or systemic infections such as invasive pulmonary aspergillosis, especially in immunocompromised patients.
30538182	5	78	theme	mpkC	862:865	arg1	mutants					876:882	mpkC deletion mutants	862:882	mpkC deletion mutants	862:882	Alterations in glycogen synthesis were observed for the sakA and mpkC deletion mutants, which also displayed alterations in carbohydrate exposure on the cell wall.
30538182	11	79	theme	fungal	2151:2156	arg1	wall					2163:2166	the fungal cell wall	2147:2166	the fungal cell wall	2147:2166	We suggest that the reduced mobilization of simple sugars causes defects in the structure of the fungal cell wall.
30538182	8	80	theme	cell	1566:1569	arg1	component					1588:1596	the main component	1579:1596	the main component responsible for recognition by the immune system, due to the specific composition of polysaccharide carbohydrates exposed on the surface of the fungal cell wall called pathogen-associated molecular patterns (PAMPs)	1579:1811	The fungal cell wall is the main component responsible for recognition by the immune system, due to the specific composition of polysaccharide carbohydrates exposed on the surface of the fungal cell wall called pathogen-associated molecular patterns (PAMPs).
30538182	8	80	theme	cell	1566:1569	arg1	wall					1571:1574	The fungal cell wall	1555:1574	The fungal cell wall	1555:1574	The fungal cell wall is the main component responsible for recognition by the immune system, due to the specific composition of polysaccharide carbohydrates exposed on the surface of the fungal cell wall called pathogen-associated molecular patterns (PAMPs).
30538182	1	81	theme	Aspergillus	148:158	arg1	MAPKs					205:209	MAPKs	205:209	MAPKs	205:209	Aspergillus fumigatus mitogen-activated protein kinases (MAPKs) are involved in maintaining the normal morphology of the cell wall and providing resistance against cell wall-damaging agents.
30538182	1	81	theme	Aspergillus	148:158	arg1	kinases					196:202	Aspergillus fumigatus mitogen-activated protein kinases	148:202	Aspergillus fumigatus mitogen-activated protein kinases (MAPKs)	148:210	Aspergillus fumigatus mitogen-activated protein kinases (MAPKs) are involved in maintaining the normal morphology of the cell wall and providing resistance against cell wall-damaging agents.
30538182	9	82	theme	cell	1840:1843	arg1	biosynthesis					1850:1861	the fungal cell wall biosynthesis	1829:1861	the fungal cell wall biosynthesis	1829:1861	Key enzymes in the fungal cell wall biosynthesis are a good target for fungal drug development.
30538182	8	83	theme	main	1583:1586	arg1	component					1588:1596	the main component	1579:1596	the main component responsible for recognition by the immune system, due to the specific composition of polysaccharide carbohydrates exposed on the surface of the fungal cell wall called pathogen-associated molecular patterns (PAMPs)	1579:1811	The fungal cell wall is the main component responsible for recognition by the immune system, due to the specific composition of polysaccharide carbohydrates exposed on the surface of the fungal cell wall called pathogen-associated molecular patterns (PAMPs).
30538182	8	83	theme	main	1583:1586	arg1	wall					1571:1574	The fungal cell wall	1555:1574	The fungal cell wall	1555:1574	The fungal cell wall is the main component responsible for recognition by the immune system, due to the specific composition of polysaccharide carbohydrates exposed on the surface of the fungal cell wall called pathogen-associated molecular patterns (PAMPs).
30538182	6	84	with	interaction	1009:1019	arg1	PkaC1					1026:1030	PkaC1	1026:1030	PkaC1	1026:1030	Carbohydrate mobilization is controlled by SakA interaction with PkaC1 and PkaR, suggesting a putative mechanism where the PkaR regulatory subunit leaves the complex and releases the SakA-PkaC1 complex for activation of enzymes involved in carbohydrate mobilization.
30538182	6	84	with	interaction	1009:1019	arg1	PkaR					1036:1039	PkaR	1036:1039	PkaR	1036:1039	Carbohydrate mobilization is controlled by SakA interaction with PkaC1 and PkaR, suggesting a putative mechanism where the PkaR regulatory subunit leaves the complex and releases the SakA-PkaC1 complex for activation of enzymes involved in carbohydrate mobilization.
30538182	7	85	theme	carbohydrate	1301:1312	arg1	mobilization					1314:1325	carbohydrate mobilization	1301:1325	carbohydrate mobilization for cell wall construction.IMPORTANCEAspergillus fumigatus	1301:1384	This work reveals the communication between the HOG and PKA pathways for carbohydrate mobilization for cell wall construction.IMPORTANCEAspergillus fumigatus is an opportunistic human pathogen causing allergic reactions or systemic infections such as invasive pulmonary aspergillosis, especially in immunocompromised patients.
30538182	7	85	theme	carbohydrate	1301:1312	arg1	pathogen					1412:1419	an opportunistic human pathogen	1389:1419	an opportunistic human pathogen causing allergic reactions or systemic infections such as invasive pulmonary aspergillosis	1389:1510	This work reveals the communication between the HOG and PKA pathways for carbohydrate mobilization for cell wall construction.IMPORTANCEAspergillus fumigatus is an opportunistic human pathogen causing allergic reactions or systemic infections such as invasive pulmonary aspergillosis, especially in immunocompromised patients.
30538182	1	86	theme	mitogen-activated	170:186	arg1	MAPKs					205:209	MAPKs	205:209	MAPKs	205:209	Aspergillus fumigatus mitogen-activated protein kinases (MAPKs) are involved in maintaining the normal morphology of the cell wall and providing resistance against cell wall-damaging agents.
30538182	1	86	theme	mitogen-activated	170:186	arg1	kinases					196:202	Aspergillus fumigatus mitogen-activated protein kinases	148:202	Aspergillus fumigatus mitogen-activated protein kinases (MAPKs)	148:210	Aspergillus fumigatus mitogen-activated protein kinases (MAPKs) are involved in maintaining the normal morphology of the cell wall and providing resistance against cell wall-damaging agents.
30538182	8	87	theme	responsible	1598:1608	arg1	component					1588:1596	the main component	1579:1596	the main component responsible for recognition by the immune system, due to the specific composition of polysaccharide carbohydrates exposed on the surface of the fungal cell wall called pathogen-associated molecular patterns (PAMPs)	1579:1811	The fungal cell wall is the main component responsible for recognition by the immune system, due to the specific composition of polysaccharide carbohydrates exposed on the surface of the fungal cell wall called pathogen-associated molecular patterns (PAMPs).
30538182	8	87	theme	responsible	1598:1608	arg1	wall					1571:1574	The fungal cell wall	1555:1574	The fungal cell wall	1555:1574	The fungal cell wall is the main component responsible for recognition by the immune system, due to the specific composition of polysaccharide carbohydrates exposed on the surface of the fungal cell wall called pathogen-associated molecular patterns (PAMPs).
30538182	7	88	theme	allergic	1429:1436	arg1	reactions					1438:1446	allergic reactions	1429:1446	allergic reactions	1429:1446	This work reveals the communication between the HOG and PKA pathways for carbohydrate mobilization for cell wall construction.IMPORTANCEAspergillus fumigatus is an opportunistic human pathogen causing allergic reactions or systemic infections such as invasive pulmonary aspergillosis, especially in immunocompromised patients.
30538182	7	88	theme	allergic	1429:1436	arg1	aspergillosis					1498:1510	invasive pulmonary aspergillosis	1479:1510	invasive pulmonary aspergillosis	1479:1510	This work reveals the communication between the HOG and PKA pathways for carbohydrate mobilization for cell wall construction.IMPORTANCEAspergillus fumigatus is an opportunistic human pathogen causing allergic reactions or systemic infections such as invasive pulmonary aspergillosis, especially in immunocompromised patients.
30538182	0	89	theme	Wall	91:94	arg1	Mobilization					109:120	Cell Wall Carbohydrate Mobilization	86:120	Cell Wall Carbohydrate Mobilization	86:120	Protein Kinase A and High-Osmolarity Glycerol Response Pathways Cooperatively Control Cell Wall Carbohydrate Mobilization in Aspergillus fumigatus.
30538182	12	90	theme	wall	2353:2356	arg1	damage					2358:2363	cell wall damage	2348:2363	cell wall damage	2348:2363	In summary, we propose that SakA is important for PKA activity, therefore regulating the availability and mobilization of monosaccharides for fungal cell wall biosynthesis during cell wall damage and the osmotic stress response.
30538182	12	91	theme	cell	2318:2321	arg1	biosynthesis					2328:2339	fungal cell wall biosynthesis	2311:2339	fungal cell wall biosynthesis	2311:2339	In summary, we propose that SakA is important for PKA activity, therefore regulating the availability and mobilization of monosaccharides for fungal cell wall biosynthesis during cell wall damage and the osmotic stress response.
30538182	5	92	from	alterations	906:916	arg1	exposure					934:941	carbohydrate exposure	921:941	carbohydrate exposure	921:941	Alterations in glycogen synthesis were observed for the sakA and mpkC deletion mutants, which also displayed alterations in carbohydrate exposure on the cell wall.
30538182	7	93	theme	human	1406:1410	arg1	mobilization					1314:1325	carbohydrate mobilization	1301:1325	carbohydrate mobilization for cell wall construction.IMPORTANCEAspergillus fumigatus	1301:1384	This work reveals the communication between the HOG and PKA pathways for carbohydrate mobilization for cell wall construction.IMPORTANCEAspergillus fumigatus is an opportunistic human pathogen causing allergic reactions or systemic infections such as invasive pulmonary aspergillosis, especially in immunocompromised patients.
30538182	7	93	theme	human	1406:1410	arg1	pathogen					1412:1419	an opportunistic human pathogen	1389:1419	an opportunistic human pathogen causing allergic reactions or systemic infections such as invasive pulmonary aspergillosis	1389:1510	This work reveals the communication between the HOG and PKA pathways for carbohydrate mobilization for cell wall construction.IMPORTANCEAspergillus fumigatus is an opportunistic human pathogen causing allergic reactions or systemic infections such as invasive pulmonary aspergillosis, especially in immunocompromised patients.
30538182	9	94	theme	fungal	1885:1890	arg1	development					1897:1907	fungal drug development	1885:1907	fungal drug development	1885:1907	Key enzymes in the fungal cell wall biosynthesis are a good target for fungal drug development.
30538182	2	95	theme	carbohydrate	415:426	arg1	compounds					436:444	carbohydrate storage compounds	415:444	carbohydrate storage compounds	415:444	Upon cell wall stress, cell wall-related sugars need to be synthesized from carbohydrate storage compounds.
30538182	0	96	theme	Kinase	8:13	arg1	A					15:15	Protein Kinase A	0:15	Protein Kinase A	0:15	Protein Kinase A and High-Osmolarity Glycerol Response Pathways Cooperatively Control Cell Wall Carbohydrate Mobilization in Aspergillus fumigatus.
30538182	6	97	theme	regulatory	1089:1098	arg1	subunit					1100:1106	the PkaR regulatory subunit	1080:1106	the PkaR regulatory subunit	1080:1106	Carbohydrate mobilization is controlled by SakA interaction with PkaC1 and PkaR, suggesting a putative mechanism where the PkaR regulatory subunit leaves the complex and releases the SakA-PkaC1 complex for activation of enzymes involved in carbohydrate mobilization.
30538182	8	98	theme	due	1648:1650	arg1	system					1640:1645	the immune system	1629:1645	the immune system	1629:1645	The fungal cell wall is the main component responsible for recognition by the immune system, due to the specific composition of polysaccharide carbohydrates exposed on the surface of the fungal cell wall called pathogen-associated molecular patterns (PAMPs).
30538182	10	99	theme	PKA	1969:1971	arg1	pathways					1973:1980	the HOG and PKA pathways	1957:1980	pathways	1973:1980	This report elucidates the cooperation between the HOG and PKA pathways in the mobilization of carbohydrates for fungal cell wall biosynthesis.
30538182	4	100	theme	genes	744:748	arg1	lack					730:733	the lack	726:733	the lack of these genes	726:748	These protein kinases are necessary for normal accumulation/degradation of trehalose and glycogen, and the lack of these genes reduces glucose uptake and glycogen synthesis.
30538182	1	101	theme	cell	312:315	arg1	agents					331:336	cell wall-damaging agents	312:336	cell wall-damaging agents	312:336	Aspergillus fumigatus mitogen-activated protein kinases (MAPKs) are involved in maintaining the normal morphology of the cell wall and providing resistance against cell wall-damaging agents.
30538182	4	102	theme	glucose	758:764	arg1	uptake					766:771	glucose uptake	758:771	glucose uptake	758:771	These protein kinases are necessary for normal accumulation/degradation of trehalose and glycogen, and the lack of these genes reduces glucose uptake and glycogen synthesis.
30538182	3	103	theme	cAMP-dependent	494:507	arg1	A					524:524	cAMP-dependent protein kinase A	494:524	cAMP-dependent protein kinase A (PKA) activity	494:539	Here we show that this process is dependent on cAMP-dependent protein kinase A (PKA) activity and regulated by the high-osmolarity glycerol response (HOG) MAPKs SakA and MpkC.
30538182	3	103	theme	cAMP-dependent	494:507	arg1	PKA					527:529	PKA	527:529	PKA	527:529	Here we show that this process is dependent on cAMP-dependent protein kinase A (PKA) activity and regulated by the high-osmolarity glycerol response (HOG) MAPKs SakA and MpkC.
30538182	0	104	theme	Glycerol	37:44	arg1	Pathways					55:62	High-Osmolarity Glycerol Response Pathways	21:62	High-Osmolarity Glycerol Response Pathways	21:62	Protein Kinase A and High-Osmolarity Glycerol Response Pathways Cooperatively Control Cell Wall Carbohydrate Mobilization in Aspergillus fumigatus.
30538182	12	105	dep	availability	2258:2269	arg1	the					2254:2256	the	2254:2256	the	2254:2256	In summary, we propose that SakA is important for PKA activity, therefore regulating the availability and mobilization of monosaccharides for fungal cell wall biosynthesis during cell wall damage and the osmotic stress response.
30538182	5	106	theme	glycogen	812:819	arg1	synthesis					821:829	glycogen synthesis	812:829	glycogen synthesis	812:829	Alterations in glycogen synthesis were observed for the sakA and mpkC deletion mutants, which also displayed alterations in carbohydrate exposure on the cell wall.
30538182	8	107	theme	polysaccharide	1683:1696	arg1	carbohydrates					1698:1710	polysaccharide carbohydrates	1683:1710	polysaccharide carbohydrates exposed on the surface of the fungal cell wall called pathogen-associated molecular patterns (PAMPs)	1683:1811	The fungal cell wall is the main component responsible for recognition by the immune system, due to the specific composition of polysaccharide carbohydrates exposed on the surface of the fungal cell wall called pathogen-associated molecular patterns (PAMPs).
30538182	2	108	theme	cell	362:365	arg1	sugars					380:385	cell wall-related sugars	362:385	cell wall-related sugars	362:385	Upon cell wall stress, cell wall-related sugars need to be synthesized from carbohydrate storage compounds.
30538182	8	109	theme	wall	1754:1757	arg1	surface					1727:1733	the surface	1723:1733	the surface of the fungal cell wall called pathogen-associated molecular patterns (PAMPs)	1723:1811	The fungal cell wall is the main component responsible for recognition by the immune system, due to the specific composition of polysaccharide carbohydrates exposed on the surface of the fungal cell wall called pathogen-associated molecular patterns (PAMPs).
30538182	6	110	theme	SakA-PkaC1	1144:1153	arg1	complex					1155:1161	the SakA-PkaC1 complex	1140:1161	the SakA-PkaC1 complex for activation of enzymes involved in carbohydrate mobilization	1140:1225	Carbohydrate mobilization is controlled by SakA interaction with PkaC1 and PkaR, suggesting a putative mechanism where the PkaR regulatory subunit leaves the complex and releases the SakA-PkaC1 complex for activation of enzymes involved in carbohydrate mobilization.
30538182	3	111	theme	kinase	517:522	arg1	A					524:524	cAMP-dependent protein kinase A	494:524	cAMP-dependent protein kinase A (PKA) activity	494:539	Here we show that this process is dependent on cAMP-dependent protein kinase A (PKA) activity and regulated by the high-osmolarity glycerol response (HOG) MAPKs SakA and MpkC.
30538182	3	111	theme	kinase	517:522	arg1	PKA					527:529	PKA	527:529	PKA	527:529	Here we show that this process is dependent on cAMP-dependent protein kinase A (PKA) activity and regulated by the high-osmolarity glycerol response (HOG) MAPKs SakA and MpkC.
30538182	8	112	theme	pathogen-associated	1766:1784	arg1	patterns					1796:1803	pathogen-associated molecular patterns	1766:1803	pathogen-associated molecular patterns (PAMPs)	1766:1811	The fungal cell wall is the main component responsible for recognition by the immune system, due to the specific composition of polysaccharide carbohydrates exposed on the surface of the fungal cell wall called pathogen-associated molecular patterns (PAMPs).
30538182	8	112	theme	pathogen-associated	1766:1784	arg1	PAMPs					1806:1810	PAMPs	1806:1810	PAMPs	1806:1810	The fungal cell wall is the main component responsible for recognition by the immune system, due to the specific composition of polysaccharide carbohydrates exposed on the surface of the fungal cell wall called pathogen-associated molecular patterns (PAMPs).
30538182	5	113	theme	deletion	867:874	arg1	mutants					876:882	mpkC deletion mutants	862:882	mpkC deletion mutants	862:882	Alterations in glycogen synthesis were observed for the sakA and mpkC deletion mutants, which also displayed alterations in carbohydrate exposure on the cell wall.
30538182	4	114	theme	protein	629:635	arg1	kinases					637:643	These protein kinases	623:643	These protein kinases	623:643	These protein kinases are necessary for normal accumulation/degradation of trehalose and glycogen, and the lack of these genes reduces glucose uptake and glycogen synthesis.
30538182	1	115	theme	cell	269:272	arg1	wall					274:277	the cell wall	265:277	the cell wall	265:277	Aspergillus fumigatus mitogen-activated protein kinases (MAPKs) are involved in maintaining the normal morphology of the cell wall and providing resistance against cell wall-damaging agents.
30758976	5	0	contain	had	1017:1019	arg1	rats					959:962	Choice pregnant rats	943:962	Choice pregnant rats	943:962	Choice pregnant rats exhibited uterine artery endothelial dysfunction and had smaller fetuses compared with chow pregnant rats.
30758976	5	0	contain	had	1017:1019	arg2	fetuses					1029:1035	smaller fetuses	1021:1035	smaller fetuses	1021:1035	Choice pregnant rats exhibited uterine artery endothelial dysfunction and had smaller fetuses compared with chow pregnant rats.
30758976	3	1	theme	smaller	679:685	arg1	weights					707:713	smaller kidney and pancreas weights	679:713	smaller kidney and pancreas weights	679:713	In addition, choice-diet-fed rats had larger cardiac ventricular weights, smaller kidney and pancreas weights, and higher blood pressure than chow-fed rats, but they did not exhibit resistance artery endothelial dysfunction.
30758976	2	2	theme	developed	574:582	arg1	intolerance					592:602	developed glucose intolerance	574:602	developed glucose intolerance	574:602	In the nonpregnant state, female Sprague-Dawley rats offered a 3-wk free-choice high-fat, high-sucrose diet had greater energy intake, adiposity, serum leptin, and triglyceride concentrations compared with rats fed with standard chow and developed glucose intolerance.
30758976	2	3	contain	had	444:446	arg2	intake					463:468	greater energy intake	448:468	greater energy intake	448:468	In the nonpregnant state, female Sprague-Dawley rats offered a 3-wk free-choice high-fat, high-sucrose diet had greater energy intake, adiposity, serum leptin, and triglyceride concentrations compared with rats fed with standard chow and developed glucose intolerance.
30758976	2	3	contain	had	444:446	arg2	concentrations					513:526	triglyceride concentrations	500:526	triglyceride concentrations	500:526	In the nonpregnant state, female Sprague-Dawley rats offered a 3-wk free-choice high-fat, high-sucrose diet had greater energy intake, adiposity, serum leptin, and triglyceride concentrations compared with rats fed with standard chow and developed glucose intolerance.
30758976	2	3	contain	had	444:446	arg1	rats					384:387	female Sprague-Dawley rats	362:387	female Sprague-Dawley rats offered a 3-wk free-choice high-fat, high-sucrose diet	362:442	In the nonpregnant state, female Sprague-Dawley rats offered a 3-wk free-choice high-fat, high-sucrose diet had greater energy intake, adiposity, serum leptin, and triglyceride concentrations compared with rats fed with standard chow and developed glucose intolerance.
30758976	2	3	contain	had	444:446	arg2	leptin					488:493	serum leptin	482:493	serum leptin	482:493	In the nonpregnant state, female Sprague-Dawley rats offered a 3-wk free-choice high-fat, high-sucrose diet had greater energy intake, adiposity, serum leptin, and triglyceride concentrations compared with rats fed with standard chow and developed glucose intolerance.
30758976	2	3	contain	had	444:446	arg2	adiposity					471:479	adiposity	471:479	adiposity	471:479	In the nonpregnant state, female Sprague-Dawley rats offered a 3-wk free-choice high-fat, high-sucrose diet had greater energy intake, adiposity, serum leptin, and triglyceride concentrations compared with rats fed with standard chow and developed glucose intolerance.
30758976	1	4	theme	free-choice	259:269	arg1	diet					294:297	a free-choice high-fat, high-sucrose diet	257:297	diet	294:297	The main objective of these studies was to characterize metabolic, body composition, and cardiovascular responses to a free-choice high-fat, high-sucrose diet in female cycling and pregnant rats.
30758976	3	5	theme	blood	727:731	arg1	pressure					733:740	higher blood pressure	720:740	higher blood pressure	720:740	In addition, choice-diet-fed rats had larger cardiac ventricular weights, smaller kidney and pancreas weights, and higher blood pressure than chow-fed rats, but they did not exhibit resistance artery endothelial dysfunction.
30758976	7	6	from	evaluation	1213:1222	arg1	functions					1299:1307	metabolic and cardiovascular functions	1270:1307	metabolic and cardiovascular functions in female rats	1270:1322	These studies are the first to provide a comprehensive evaluation of free-choice high-fat, high-sucrose diet on metabolic and cardiovascular functions in female rats, extending the previous studies in males to female cycling and pregnant rodents.
30758976	0	7	theme	pregnant	125:132	arg1	rats					134:137	female cycling and pregnant rats	106:137	rats	134:137	A free-choice high-fat, high-sucrose diet induces hyperphagia, obesity, and cardiovascular dysfunction in female cycling and pregnant rats.
30758976	5	8	theme	pregnant	1056:1063	arg1	rats					1065:1068	chow pregnant rats	1051:1068	chow pregnant rats	1051:1068	Choice pregnant rats exhibited uterine artery endothelial dysfunction and had smaller fetuses compared with chow pregnant rats.
30758976	1	9	from	composition	212:222	arg1	rats					330:333	female cycling and pregnant rats	302:333	rats	330:333	The main objective of these studies was to characterize metabolic, body composition, and cardiovascular responses to a free-choice high-fat, high-sucrose diet in female cycling and pregnant rats.
30758976	1	9	from	composition	212:222	arg1	cycling					309:315	female cycling and pregnant rats	302:333	cycling	309:315	The main objective of these studies was to characterize metabolic, body composition, and cardiovascular responses to a free-choice high-fat, high-sucrose diet in female cycling and pregnant rats.
30758976	2	10	theme	serum	482:486	arg1	leptin					488:493	serum leptin	482:493	serum leptin	482:493	In the nonpregnant state, female Sprague-Dawley rats offered a 3-wk free-choice high-fat, high-sucrose diet had greater energy intake, adiposity, serum leptin, and triglyceride concentrations compared with rats fed with standard chow and developed glucose intolerance.
30758976	8	11	theme	increased	1501:1509	arg1	intake					1518:1523	increased energy intake	1501:1523	increased energy intake	1501:1523	Free-choice diet may provide a new model of preconceptual maternal obesity to study the role of increased energy intake, individual food components, and preexisting maternal obesity on maternal and offspring physiological responses during pregnancy and after birth.
30758976	7	12	theme	female	1368:1373	arg1	cycling					1375:1381	female cycling and pregnant rodents	1368:1402	cycling	1375:1381	These studies are the first to provide a comprehensive evaluation of free-choice high-fat, high-sucrose diet on metabolic and cardiovascular functions in female rats, extending the previous studies in males to female cycling and pregnant rodents.
30758976	7	13	theme	diet	1262:1265	arg1	evaluation					1213:1222	a comprehensive evaluation	1197:1222	a comprehensive evaluation of free-choice high-fat, high-sucrose diet on metabolic and cardiovascular functions in female rats	1197:1322	These studies are the first to provide a comprehensive evaluation of free-choice high-fat, high-sucrose diet on metabolic and cardiovascular functions in female rats, extending the previous studies in males to female cycling and pregnant rodents.
30758976	8	14	theme	intake	1518:1523	arg1	role					1493:1496	the role	1489:1496	the role of increased energy intake, individual food components, and preexisting maternal obesity on maternal and offspring physiological responses	1489:1635	Free-choice diet may provide a new model of preconceptual maternal obesity to study the role of increased energy intake, individual food components, and preexisting maternal obesity on maternal and offspring physiological responses during pregnancy and after birth.
30758976	0	15	from	dysfunction	91:101	arg1	cycling					113:119	female cycling and pregnant rats	106:137	cycling	113:119	A free-choice high-fat, high-sucrose diet induces hyperphagia, obesity, and cardiovascular dysfunction in female cycling and pregnant rats.
30758976	0	15	from	dysfunction	91:101	arg1	rats					134:137	female cycling and pregnant rats	106:137	rats	134:137	A free-choice high-fat, high-sucrose diet induces hyperphagia, obesity, and cardiovascular dysfunction in female cycling and pregnant rats.
30758976	2	16	theme	triglyceride	500:511	arg1	concentrations					513:526	triglyceride concentrations	500:526	triglyceride concentrations	500:526	In the nonpregnant state, female Sprague-Dawley rats offered a 3-wk free-choice high-fat, high-sucrose diet had greater energy intake, adiposity, serum leptin, and triglyceride concentrations compared with rats fed with standard chow and developed glucose intolerance.
30758976	8	17	theme	individual	1526:1535	arg1	components					1542:1551	individual food components	1526:1551	individual food components	1526:1551	Free-choice diet may provide a new model of preconceptual maternal obesity to study the role of increased energy intake, individual food components, and preexisting maternal obesity on maternal and offspring physiological responses during pregnancy and after birth.
30758976	3	18	theme	artery	798:803	arg1	dysfunction					817:827	resistance artery endothelial dysfunction	787:827	resistance artery endothelial dysfunction	787:827	In addition, choice-diet-fed rats had larger cardiac ventricular weights, smaller kidney and pancreas weights, and higher blood pressure than chow-fed rats, but they did not exhibit resistance artery endothelial dysfunction.
30758976	0	19	theme	cardiovascular	76:89	arg1	dysfunction					91:101	cardiovascular dysfunction	76:101	cardiovascular dysfunction	76:101	A free-choice high-fat, high-sucrose diet induces hyperphagia, obesity, and cardiovascular dysfunction in female cycling and pregnant rats.
30758976	5	20	theme	uterine	974:980	arg1	dysfunction					1001:1011	uterine artery endothelial dysfunction	974:1011	uterine artery endothelial dysfunction	974:1011	Choice pregnant rats exhibited uterine artery endothelial dysfunction and had smaller fetuses compared with chow pregnant rats.
30758976	8	21	theme	components	1542:1551	arg1	role					1493:1496	the role	1489:1496	the role of increased energy intake, individual food components, and preexisting maternal obesity on maternal and offspring physiological responses	1489:1635	Free-choice diet may provide a new model of preconceptual maternal obesity to study the role of increased energy intake, individual food components, and preexisting maternal obesity on maternal and offspring physiological responses during pregnancy and after birth.
30758976	8	22	from	role	1493:1496	arg1	responses					1627:1635	maternal and offspring physiological responses	1590:1635	maternal and offspring physiological responses	1590:1635	Free-choice diet may provide a new model of preconceptual maternal obesity to study the role of increased energy intake, individual food components, and preexisting maternal obesity on maternal and offspring physiological responses during pregnancy and after birth.
30758976	5	23	theme	endothelial	989:999	arg1	dysfunction					1001:1011	uterine artery endothelial dysfunction	974:1011	uterine artery endothelial dysfunction	974:1011	Choice pregnant rats exhibited uterine artery endothelial dysfunction and had smaller fetuses compared with chow pregnant rats.
30758976	6	24	theme	arterial	1097:1104	arg1	pressure					1112:1119	mean arterial blood pressure	1092:1119	mean arterial blood pressure	1092:1119	Pregnancy normalized mean arterial blood pressure and pancreas weights in choice rats.
30758976	2	25	theme	female	362:367	arg1	rats					384:387	female Sprague-Dawley rats	362:387	female Sprague-Dawley rats offered a 3-wk free-choice high-fat, high-sucrose diet	362:442	In the nonpregnant state, female Sprague-Dawley rats offered a 3-wk free-choice high-fat, high-sucrose diet had greater energy intake, adiposity, serum leptin, and triglyceride concentrations compared with rats fed with standard chow and developed glucose intolerance.
30758976	3	26	dep	smaller	679:685	arg1	kidney					687:692	kidney	687:692	kidney	687:692	In addition, choice-diet-fed rats had larger cardiac ventricular weights, smaller kidney and pancreas weights, and higher blood pressure than chow-fed rats, but they did not exhibit resistance artery endothelial dysfunction.
30758976	5	27	theme	Choice	943:948	arg1	rats					959:962	Choice pregnant rats	943:962	Choice pregnant rats	943:962	Choice pregnant rats exhibited uterine artery endothelial dysfunction and had smaller fetuses compared with chow pregnant rats.
30758976	8	28	theme	maternal	1570:1577	arg1	obesity					1579:1585	preexisting maternal obesity	1558:1585	preexisting maternal obesity	1558:1585	Free-choice diet may provide a new model of preconceptual maternal obesity to study the role of increased energy intake, individual food components, and preexisting maternal obesity on maternal and offspring physiological responses during pregnancy and after birth.
30758976	2	29	theme	free-choice	404:414	arg1	high-fat					416:423	a 3-wk free-choice high-fat	397:423	a 3-wk free-choice high-fat	397:423	In the nonpregnant state, female Sprague-Dawley rats offered a 3-wk free-choice high-fat, high-sucrose diet had greater energy intake, adiposity, serum leptin, and triglyceride concentrations compared with rats fed with standard chow and developed glucose intolerance.
30758976	2	29	theme	free-choice	404:414	arg1	diet					439:442	high-sucrose diet	426:442	high-sucrose diet	426:442	In the nonpregnant state, female Sprague-Dawley rats offered a 3-wk free-choice high-fat, high-sucrose diet had greater energy intake, adiposity, serum leptin, and triglyceride concentrations compared with rats fed with standard chow and developed glucose intolerance.
30758976	0	30	theme	high-fat	14:21	arg1	diet					37:40	A free-choice high-fat, high-sucrose diet	0:40	diet	37:40	A free-choice high-fat, high-sucrose diet induces hyperphagia, obesity, and cardiovascular dysfunction in female cycling and pregnant rats.
30758976	7	31	theme	previous	1339:1346	arg1	studies					1348:1354	the previous studies	1335:1354	the previous studies in males	1335:1363	These studies are the first to provide a comprehensive evaluation of free-choice high-fat, high-sucrose diet on metabolic and cardiovascular functions in female rats, extending the previous studies in males to female cycling and pregnant rodents.
30758976	3	32	theme	larger	643:648	arg1	weights					670:676	larger cardiac ventricular weights	643:676	larger cardiac ventricular weights	643:676	In addition, choice-diet-fed rats had larger cardiac ventricular weights, smaller kidney and pancreas weights, and higher blood pressure than chow-fed rats, but they did not exhibit resistance artery endothelial dysfunction.
30758976	2	33	theme	greater	448:454	arg1	intake					463:468	greater energy intake	448:468	greater energy intake	448:468	In the nonpregnant state, female Sprague-Dawley rats offered a 3-wk free-choice high-fat, high-sucrose diet had greater energy intake, adiposity, serum leptin, and triglyceride concentrations compared with rats fed with standard chow and developed glucose intolerance.
30758976	8	34	theme	new	1436:1438	arg1	model					1440:1444	a new model	1434:1444	a new model of preconceptual maternal obesity to study the role of increased energy intake, individual food components, and preexisting maternal obesity on maternal and offspring physiological responses during pregnancy and after birth	1434:1668	Free-choice diet may provide a new model of preconceptual maternal obesity to study the role of increased energy intake, individual food components, and preexisting maternal obesity on maternal and offspring physiological responses during pregnancy and after birth.
30758976	1	35	theme	female	302:307	arg1	cycling					309:315	female cycling and pregnant rats	302:333	cycling	309:315	The main objective of these studies was to characterize metabolic, body composition, and cardiovascular responses to a free-choice high-fat, high-sucrose diet in female cycling and pregnant rats.
30758976	5	36	theme	chow	1051:1054	arg1	rats					1065:1068	chow pregnant rats	1051:1068	chow pregnant rats	1051:1068	Choice pregnant rats exhibited uterine artery endothelial dysfunction and had smaller fetuses compared with chow pregnant rats.
30758976	8	37	theme	physiological	1613:1625	arg1	responses					1627:1635	maternal and offspring physiological responses	1590:1635	maternal and offspring physiological responses	1590:1635	Free-choice diet may provide a new model of preconceptual maternal obesity to study the role of increased energy intake, individual food components, and preexisting maternal obesity on maternal and offspring physiological responses during pregnancy and after birth.
30758976	1	38	theme	body	207:210	arg1	composition					212:222	body composition	207:222	body composition	207:222	The main objective of these studies was to characterize metabolic, body composition, and cardiovascular responses to a free-choice high-fat, high-sucrose diet in female cycling and pregnant rats.
30758976	7	39	from	studies	1348:1354	arg1	males					1359:1363	males	1359:1363	males	1359:1363	These studies are the first to provide a comprehensive evaluation of free-choice high-fat, high-sucrose diet on metabolic and cardiovascular functions in female rats, extending the previous studies in males to female cycling and pregnant rodents.
30758976	3	40	theme	ventricular	658:668	arg1	weights					670:676	larger cardiac ventricular weights	643:676	larger cardiac ventricular weights	643:676	In addition, choice-diet-fed rats had larger cardiac ventricular weights, smaller kidney and pancreas weights, and higher blood pressure than chow-fed rats, but they did not exhibit resistance artery endothelial dysfunction.
30758976	8	41	theme	maternal	1463:1470	arg1	obesity					1472:1478	preconceptual maternal obesity	1449:1478	preconceptual maternal obesity	1449:1478	Free-choice diet may provide a new model of preconceptual maternal obesity to study the role of increased energy intake, individual food components, and preexisting maternal obesity on maternal and offspring physiological responses during pregnancy and after birth.
30758976	6	42	from	pressure	1112:1119	arg1	rats					1152:1155	choice rats	1145:1155	choice rats	1145:1155	Pregnancy normalized mean arterial blood pressure and pancreas weights in choice rats.
30758976	7	43	theme	comprehensive	1199:1211	arg1	evaluation					1213:1222	a comprehensive evaluation	1197:1222	a comprehensive evaluation of free-choice high-fat, high-sucrose diet on metabolic and cardiovascular functions in female rats	1197:1322	These studies are the first to provide a comprehensive evaluation of free-choice high-fat, high-sucrose diet on metabolic and cardiovascular functions in female rats, extending the previous studies in males to female cycling and pregnant rodents.
30758976	5	44	theme	smaller	1021:1027	arg1	fetuses					1029:1035	smaller fetuses	1021:1035	smaller fetuses	1021:1035	Choice pregnant rats exhibited uterine artery endothelial dysfunction and had smaller fetuses compared with chow pregnant rats.
30758976	7	45	theme	cardiovascular	1284:1297	arg1	functions					1299:1307	metabolic and cardiovascular functions	1270:1307	metabolic and cardiovascular functions in female rats	1270:1322	These studies are the first to provide a comprehensive evaluation of free-choice high-fat, high-sucrose diet on metabolic and cardiovascular functions in female rats, extending the previous studies in males to female cycling and pregnant rodents.
30758976	3	46	theme	pancreas	698:705	arg1	weights					707:713	smaller kidney and pancreas weights	679:713	smaller kidney and pancreas weights	679:713	In addition, choice-diet-fed rats had larger cardiac ventricular weights, smaller kidney and pancreas weights, and higher blood pressure than chow-fed rats, but they did not exhibit resistance artery endothelial dysfunction.
30758976	1	47	theme	cardiovascular	229:242	arg1	responses					244:252	cardiovascular responses	229:252	cardiovascular responses to a free-choice high-fat, high-sucrose diet in female cycling and pregnant rats	229:333	The main objective of these studies was to characterize metabolic, body composition, and cardiovascular responses to a free-choice high-fat, high-sucrose diet in female cycling and pregnant rats.
30758976	2	48	theme	glucose	584:590	arg1	intolerance					592:602	developed glucose intolerance	574:602	developed glucose intolerance	574:602	In the nonpregnant state, female Sprague-Dawley rats offered a 3-wk free-choice high-fat, high-sucrose diet had greater energy intake, adiposity, serum leptin, and triglyceride concentrations compared with rats fed with standard chow and developed glucose intolerance.
30758976	7	49	theme	metabolic	1270:1278	arg1	functions					1299:1307	metabolic and cardiovascular functions	1270:1307	metabolic and cardiovascular functions in female rats	1270:1322	These studies are the first to provide a comprehensive evaluation of free-choice high-fat, high-sucrose diet on metabolic and cardiovascular functions in female rats, extending the previous studies in males to female cycling and pregnant rodents.
30758976	3	50	theme	higher	720:725	arg1	pressure					733:740	higher blood pressure	720:740	higher blood pressure	720:740	In addition, choice-diet-fed rats had larger cardiac ventricular weights, smaller kidney and pancreas weights, and higher blood pressure than chow-fed rats, but they did not exhibit resistance artery endothelial dysfunction.
30758976	7	51	from	functions	1299:1307	arg1	rats					1319:1322	female rats	1312:1322	female rats	1312:1322	These studies are the first to provide a comprehensive evaluation of free-choice high-fat, high-sucrose diet on metabolic and cardiovascular functions in female rats, extending the previous studies in males to female cycling and pregnant rodents.
30758976	1	52	theme	studies	168:174	arg1	objective					149:157	The main objective	140:157	The main objective of these studies	140:174	The main objective of these studies was to characterize metabolic, body composition, and cardiovascular responses to a free-choice high-fat, high-sucrose diet in female cycling and pregnant rats.
30758976	4	53	theme	free-choice	839:849	arg1	diet					851:854	the free-choice diet	835:854	the free-choice diet	835:854	When the free-choice diet continued throughout pregnancy, rats remained hyperphagic, hyperleptinemic, and obese.
30758976	3	54	theme	chow-fed	747:754	arg1	rats					756:759	chow-fed rats	747:759	chow-fed rats	747:759	In addition, choice-diet-fed rats had larger cardiac ventricular weights, smaller kidney and pancreas weights, and higher blood pressure than chow-fed rats, but they did not exhibit resistance artery endothelial dysfunction.
30758976	1	55	theme	metabolic	196:204	arg1	composition					212:222	body composition	207:222	body composition	207:222	The main objective of these studies was to characterize metabolic, body composition, and cardiovascular responses to a free-choice high-fat, high-sucrose diet in female cycling and pregnant rats.
30758976	0	56	from	obesity	63:69	arg1	cycling					113:119	female cycling and pregnant rats	106:137	cycling	113:119	A free-choice high-fat, high-sucrose diet induces hyperphagia, obesity, and cardiovascular dysfunction in female cycling and pregnant rats.
30758976	0	56	from	obesity	63:69	arg1	rats					134:137	female cycling and pregnant rats	106:137	rats	134:137	A free-choice high-fat, high-sucrose diet induces hyperphagia, obesity, and cardiovascular dysfunction in female cycling and pregnant rats.
30758976	3	57	contain	had	639:641	arg2	weights					670:676	larger cardiac ventricular weights	643:676	larger cardiac ventricular weights	643:676	In addition, choice-diet-fed rats had larger cardiac ventricular weights, smaller kidney and pancreas weights, and higher blood pressure than chow-fed rats, but they did not exhibit resistance artery endothelial dysfunction.
30758976	3	57	contain	had	639:641	arg1	rats					634:637	choice-diet-fed rats	618:637	choice-diet-fed rats	618:637	In addition, choice-diet-fed rats had larger cardiac ventricular weights, smaller kidney and pancreas weights, and higher blood pressure than chow-fed rats, but they did not exhibit resistance artery endothelial dysfunction.
30758976	3	57	contain	had	639:641	arg2	pressure					733:740	higher blood pressure	720:740	higher blood pressure	720:740	In addition, choice-diet-fed rats had larger cardiac ventricular weights, smaller kidney and pancreas weights, and higher blood pressure than chow-fed rats, but they did not exhibit resistance artery endothelial dysfunction.
30758976	3	57	contain	had	639:641	arg2	weights					707:713	smaller kidney and pancreas weights	679:713	smaller kidney and pancreas weights	679:713	In addition, choice-diet-fed rats had larger cardiac ventricular weights, smaller kidney and pancreas weights, and higher blood pressure than chow-fed rats, but they did not exhibit resistance artery endothelial dysfunction.
30758976	2	58	theme	standard	556:563	arg1	chow					565:568	standard chow	556:568	standard chow	556:568	In the nonpregnant state, female Sprague-Dawley rats offered a 3-wk free-choice high-fat, high-sucrose diet had greater energy intake, adiposity, serum leptin, and triglyceride concentrations compared with rats fed with standard chow and developed glucose intolerance.
30758976	8	59	theme	energy	1511:1516	arg1	intake					1518:1523	increased energy intake	1501:1523	increased energy intake	1501:1523	Free-choice diet may provide a new model of preconceptual maternal obesity to study the role of increased energy intake, individual food components, and preexisting maternal obesity on maternal and offspring physiological responses during pregnancy and after birth.
30758976	7	60	theme	pregnant	1387:1394	arg1	rodents					1396:1402	female cycling and pregnant rodents	1368:1402	rodents	1396:1402	These studies are the first to provide a comprehensive evaluation of free-choice high-fat, high-sucrose diet on metabolic and cardiovascular functions in female rats, extending the previous studies in males to female cycling and pregnant rodents.
30758976	3	61	theme	resistance	787:796	arg1	dysfunction					817:827	resistance artery endothelial dysfunction	787:827	resistance artery endothelial dysfunction	787:827	In addition, choice-diet-fed rats had larger cardiac ventricular weights, smaller kidney and pancreas weights, and higher blood pressure than chow-fed rats, but they did not exhibit resistance artery endothelial dysfunction.
30758976	7	62	theme	free-choice	1227:1237	arg1	diet					1262:1265	free-choice high-fat, high-sucrose diet	1227:1265	diet	1262:1265	These studies are the first to provide a comprehensive evaluation of free-choice high-fat, high-sucrose diet on metabolic and cardiovascular functions in female rats, extending the previous studies in males to female cycling and pregnant rodents.
30758976	7	63	theme	high-fat	1239:1246	arg1	diet					1262:1265	free-choice high-fat, high-sucrose diet	1227:1265	diet	1262:1265	These studies are the first to provide a comprehensive evaluation of free-choice high-fat, high-sucrose diet on metabolic and cardiovascular functions in female rats, extending the previous studies in males to female cycling and pregnant rodents.
30758976	1	64	theme	pregnant	321:328	arg1	rats					330:333	female cycling and pregnant rats	302:333	rats	330:333	The main objective of these studies was to characterize metabolic, body composition, and cardiovascular responses to a free-choice high-fat, high-sucrose diet in female cycling and pregnant rats.
30758976	1	65	theme	main	144:147	arg1	objective					149:157	The main objective	140:157	The main objective of these studies	140:174	The main objective of these studies was to characterize metabolic, body composition, and cardiovascular responses to a free-choice high-fat, high-sucrose diet in female cycling and pregnant rats.
30758976	8	66	theme	food	1537:1540	arg1	components					1542:1551	individual food components	1526:1551	individual food components	1526:1551	Free-choice diet may provide a new model of preconceptual maternal obesity to study the role of increased energy intake, individual food components, and preexisting maternal obesity on maternal and offspring physiological responses during pregnancy and after birth.
30758976	0	67	from	hyperphagia	50:60	arg1	cycling					113:119	female cycling and pregnant rats	106:137	cycling	113:119	A free-choice high-fat, high-sucrose diet induces hyperphagia, obesity, and cardiovascular dysfunction in female cycling and pregnant rats.
30758976	0	67	from	hyperphagia	50:60	arg1	rats					134:137	female cycling and pregnant rats	106:137	rats	134:137	A free-choice high-fat, high-sucrose diet induces hyperphagia, obesity, and cardiovascular dysfunction in female cycling and pregnant rats.
30758976	3	68	theme	endothelial	805:815	arg1	dysfunction					817:827	resistance artery endothelial dysfunction	787:827	resistance artery endothelial dysfunction	787:827	In addition, choice-diet-fed rats had larger cardiac ventricular weights, smaller kidney and pancreas weights, and higher blood pressure than chow-fed rats, but they did not exhibit resistance artery endothelial dysfunction.
30758976	0	69	theme	female	106:111	arg1	cycling					113:119	female cycling and pregnant rats	106:137	cycling	113:119	A free-choice high-fat, high-sucrose diet induces hyperphagia, obesity, and cardiovascular dysfunction in female cycling and pregnant rats.
30758976	5	70	theme	artery	982:987	arg1	dysfunction					1001:1011	uterine artery endothelial dysfunction	974:1011	uterine artery endothelial dysfunction	974:1011	Choice pregnant rats exhibited uterine artery endothelial dysfunction and had smaller fetuses compared with chow pregnant rats.
30758976	6	71	theme	blood	1106:1110	arg1	pressure					1112:1119	mean arterial blood pressure	1092:1119	mean arterial blood pressure	1092:1119	Pregnancy normalized mean arterial blood pressure and pancreas weights in choice rats.
30758976	1	72	dep	diet	294:297	arg1	high-sucrose					281:292	a free-choice high-fat, high-sucrose diet	257:297	high-sucrose	281:292	The main objective of these studies was to characterize metabolic, body composition, and cardiovascular responses to a free-choice high-fat, high-sucrose diet in female cycling and pregnant rats.
30758976	2	73	theme	Sprague-Dawley	369:382	arg1	rats					384:387	female Sprague-Dawley rats	362:387	female Sprague-Dawley rats offered a 3-wk free-choice high-fat, high-sucrose diet	362:442	In the nonpregnant state, female Sprague-Dawley rats offered a 3-wk free-choice high-fat, high-sucrose diet had greater energy intake, adiposity, serum leptin, and triglyceride concentrations compared with rats fed with standard chow and developed glucose intolerance.
30758976	0	74	theme	free-choice	2:12	arg1	diet					37:40	A free-choice high-fat, high-sucrose diet	0:40	diet	37:40	A free-choice high-fat, high-sucrose diet induces hyperphagia, obesity, and cardiovascular dysfunction in female cycling and pregnant rats.
30758976	6	75	theme	mean	1092:1095	arg1	pressure					1112:1119	mean arterial blood pressure	1092:1119	mean arterial blood pressure	1092:1119	Pregnancy normalized mean arterial blood pressure and pancreas weights in choice rats.
30758976	2	76	theme	high-sucrose	426:437	arg1	high-fat					416:423	a 3-wk free-choice high-fat	397:423	a 3-wk free-choice high-fat	397:423	In the nonpregnant state, female Sprague-Dawley rats offered a 3-wk free-choice high-fat, high-sucrose diet had greater energy intake, adiposity, serum leptin, and triglyceride concentrations compared with rats fed with standard chow and developed glucose intolerance.
30758976	2	76	theme	high-sucrose	426:437	arg1	diet					439:442	high-sucrose diet	426:442	high-sucrose diet	426:442	In the nonpregnant state, female Sprague-Dawley rats offered a 3-wk free-choice high-fat, high-sucrose diet had greater energy intake, adiposity, serum leptin, and triglyceride concentrations compared with rats fed with standard chow and developed glucose intolerance.
30758976	8	77	theme	obesity	1579:1585	arg1	role					1493:1496	the role	1489:1496	the role of increased energy intake, individual food components, and preexisting maternal obesity on maternal and offspring physiological responses	1489:1635	Free-choice diet may provide a new model of preconceptual maternal obesity to study the role of increased energy intake, individual food components, and preexisting maternal obesity on maternal and offspring physiological responses during pregnancy and after birth.
30758976	8	78	theme	obesity	1472:1478	arg1	model					1440:1444	a new model	1434:1444	a new model of preconceptual maternal obesity to study the role of increased energy intake, individual food components, and preexisting maternal obesity on maternal and offspring physiological responses during pregnancy and after birth	1434:1668	Free-choice diet may provide a new model of preconceptual maternal obesity to study the role of increased energy intake, individual food components, and preexisting maternal obesity on maternal and offspring physiological responses during pregnancy and after birth.
30758976	1	79	theme	high-fat	271:278	arg1	diet					294:297	a free-choice high-fat, high-sucrose diet	257:297	diet	294:297	The main objective of these studies was to characterize metabolic, body composition, and cardiovascular responses to a free-choice high-fat, high-sucrose diet in female cycling and pregnant rats.
30758976	5	80	theme	pregnant	950:957	arg1	rats					959:962	Choice pregnant rats	943:962	Choice pregnant rats	943:962	Choice pregnant rats exhibited uterine artery endothelial dysfunction and had smaller fetuses compared with chow pregnant rats.
30758976	8	81	theme	maternal	1590:1597	arg1	responses					1627:1635	maternal and offspring physiological responses	1590:1635	maternal and offspring physiological responses	1590:1635	Free-choice diet may provide a new model of preconceptual maternal obesity to study the role of increased energy intake, individual food components, and preexisting maternal obesity on maternal and offspring physiological responses during pregnancy and after birth.
30758976	8	82	theme	Free-choice	1405:1415	arg1	diet					1417:1420	Free-choice diet	1405:1420	Free-choice diet	1405:1420	Free-choice diet may provide a new model of preconceptual maternal obesity to study the role of increased energy intake, individual food components, and preexisting maternal obesity on maternal and offspring physiological responses during pregnancy and after birth.
30758976	2	83	theme	3-wk	399:402	arg1	high-fat					416:423	a 3-wk free-choice high-fat	397:423	a 3-wk free-choice high-fat	397:423	In the nonpregnant state, female Sprague-Dawley rats offered a 3-wk free-choice high-fat, high-sucrose diet had greater energy intake, adiposity, serum leptin, and triglyceride concentrations compared with rats fed with standard chow and developed glucose intolerance.
30758976	2	83	theme	3-wk	399:402	arg1	diet					439:442	high-sucrose diet	426:442	high-sucrose diet	426:442	In the nonpregnant state, female Sprague-Dawley rats offered a 3-wk free-choice high-fat, high-sucrose diet had greater energy intake, adiposity, serum leptin, and triglyceride concentrations compared with rats fed with standard chow and developed glucose intolerance.
30758976	2	84	theme	energy	456:461	arg1	intake					463:468	greater energy intake	448:468	greater energy intake	448:468	In the nonpregnant state, female Sprague-Dawley rats offered a 3-wk free-choice high-fat, high-sucrose diet had greater energy intake, adiposity, serum leptin, and triglyceride concentrations compared with rats fed with standard chow and developed glucose intolerance.
30758976	8	85	theme	offspring	1603:1611	arg1	responses					1627:1635	maternal and offspring physiological responses	1590:1635	maternal and offspring physiological responses	1590:1635	Free-choice diet may provide a new model of preconceptual maternal obesity to study the role of increased energy intake, individual food components, and preexisting maternal obesity on maternal and offspring physiological responses during pregnancy and after birth.
30758976	7	86	dep	diet	1262:1265	arg1	high-sucrose					1249:1260	free-choice high-fat, high-sucrose diet	1227:1265	high-sucrose	1249:1260	These studies are the first to provide a comprehensive evaluation of free-choice high-fat, high-sucrose diet on metabolic and cardiovascular functions in female rats, extending the previous studies in males to female cycling and pregnant rodents.
30758976	3	87	theme	choice-diet-fed	618:632	arg1	rats					634:637	choice-diet-fed rats	618:637	choice-diet-fed rats	618:637	In addition, choice-diet-fed rats had larger cardiac ventricular weights, smaller kidney and pancreas weights, and higher blood pressure than chow-fed rats, but they did not exhibit resistance artery endothelial dysfunction.
30758976	0	88	dep	diet	37:40	arg1	high-sucrose					24:35	A free-choice high-fat, high-sucrose diet	0:40	high-sucrose	24:35	A free-choice high-fat, high-sucrose diet induces hyperphagia, obesity, and cardiovascular dysfunction in female cycling and pregnant rats.
30758976	8	89	theme	preexisting	1558:1568	arg1	obesity					1579:1585	preexisting maternal obesity	1558:1585	preexisting maternal obesity	1558:1585	Free-choice diet may provide a new model of preconceptual maternal obesity to study the role of increased energy intake, individual food components, and preexisting maternal obesity on maternal and offspring physiological responses during pregnancy and after birth.
30758976	7	90	theme	female	1312:1317	arg1	rats					1319:1322	female rats	1312:1322	female rats	1312:1322	These studies are the first to provide a comprehensive evaluation of free-choice high-fat, high-sucrose diet on metabolic and cardiovascular functions in female rats, extending the previous studies in males to female cycling and pregnant rodents.
30758976	1	91	from	responses	244:252	arg1	rats					330:333	female cycling and pregnant rats	302:333	rats	330:333	The main objective of these studies was to characterize metabolic, body composition, and cardiovascular responses to a free-choice high-fat, high-sucrose diet in female cycling and pregnant rats.
30758976	1	91	from	responses	244:252	arg1	cycling					309:315	female cycling and pregnant rats	302:333	cycling	309:315	The main objective of these studies was to characterize metabolic, body composition, and cardiovascular responses to a free-choice high-fat, high-sucrose diet in female cycling and pregnant rats.
30758976	6	92	theme	choice	1145:1150	arg1	rats					1152:1155	choice rats	1145:1155	choice rats	1145:1155	Pregnancy normalized mean arterial blood pressure and pancreas weights in choice rats.
30758976	3	93	theme	cardiac	650:656	arg1	weights					670:676	larger cardiac ventricular weights	643:676	larger cardiac ventricular weights	643:676	In addition, choice-diet-fed rats had larger cardiac ventricular weights, smaller kidney and pancreas weights, and higher blood pressure than chow-fed rats, but they did not exhibit resistance artery endothelial dysfunction.
30758976	6	94	theme	pancreas	1125:1132	arg1	weights					1134:1140	pancreas weights	1125:1140	pancreas weights	1125:1140	Pregnancy normalized mean arterial blood pressure and pancreas weights in choice rats.
30758976	8	95	theme	preconceptual	1449:1461	arg1	obesity					1472:1478	preconceptual maternal obesity	1449:1478	preconceptual maternal obesity	1449:1478	Free-choice diet may provide a new model of preconceptual maternal obesity to study the role of increased energy intake, individual food components, and preexisting maternal obesity on maternal and offspring physiological responses during pregnancy and after birth.
30758976	6	96	from	weights	1134:1140	arg1	rats					1152:1155	choice rats	1145:1155	choice rats	1145:1155	Pregnancy normalized mean arterial blood pressure and pancreas weights in choice rats.
30758976	2	97	theme	nonpregnant	343:353	arg1	state					355:359	the nonpregnant state	339:359	the nonpregnant state	339:359	In the nonpregnant state, female Sprague-Dawley rats offered a 3-wk free-choice high-fat, high-sucrose diet had greater energy intake, adiposity, serum leptin, and triglyceride concentrations compared with rats fed with standard chow and developed glucose intolerance.
29501171	3	0	theme	specific	699:706	arg1	area					716:719	large specific surface area	693:719	large specific surface area of 277.1 cm2 g-1	693:736	The obtained nano-composites were proven to have many unique properties such as large specific surface area of 277.1 cm2 g-1, uniform mesopores with average pore size of 3.35 nm, and carbon-functionalized pore-walls.
29501171	3	1	with	area	716:719	arg1	size					775:778	average pore size	762:778	average pore size of 3.35 nm	762:789	The obtained nano-composites were proven to have many unique properties such as large specific surface area of 277.1 cm2 g-1, uniform mesopores with average pore size of 3.35 nm, and carbon-functionalized pore-walls.
29501171	4	2	theme	α-glucosidase	906:918	arg1	miglitol					932:939	miglitol	932:939	miglitol	932:939	Taking advantage of the hydrophilic interaction between carbon and glycans, α-glucosidase inhibitors (miglitol and voglibose) could be directly extracted from rat plasma with no need of other pre-treatment procedures.
29501171	4	2	theme	α-glucosidase	906:918	arg1	inhibitors					920:929	α-glucosidase inhibitors	906:929	α-glucosidase inhibitors (miglitol and voglibose)	906:954	Taking advantage of the hydrophilic interaction between carbon and glycans, α-glucosidase inhibitors (miglitol and voglibose) could be directly extracted from rat plasma with no need of other pre-treatment procedures.
29501171	4	2	theme	α-glucosidase	906:918	arg1	voglibose					945:953	voglibose	945:953	voglibose	945:953	Taking advantage of the hydrophilic interaction between carbon and glycans, α-glucosidase inhibitors (miglitol and voglibose) could be directly extracted from rat plasma with no need of other pre-treatment procedures.
29501171	4	3	theme	interaction	866:876	arg1	advantage					837:845	advantage	837:845	advantage of the hydrophilic interaction between carbon and glycans	837:903	Taking advantage of the hydrophilic interaction between carbon and glycans, α-glucosidase inhibitors (miglitol and voglibose) could be directly extracted from rat plasma with no need of other pre-treatment procedures.
29501171	3	4	theme	277.1 cm2	724:732	arg1	g-1					734:736	277.1 cm2 g-1	724:736	277.1 cm2 g-1	724:736	The obtained nano-composites were proven to have many unique properties such as large specific surface area of 277.1 cm2 g-1, uniform mesopores with average pore size of 3.35 nm, and carbon-functionalized pore-walls.
29501171	3	5	theme	average	762:768	arg1	size					775:778	average pore size	762:778	average pore size of 3.35 nm	762:789	The obtained nano-composites were proven to have many unique properties such as large specific surface area of 277.1 cm2 g-1, uniform mesopores with average pore size of 3.35 nm, and carbon-functionalized pore-walls.
29501171	1	6	theme	carbon-functionalized	216:236	arg1	pore-walls					238:247	carbon-functionalized pore-walls	216:247	carbon-functionalized pore-walls (denoted as MG@mSiO2-C composites)	216:282	In this work, magnetic graphene/mesoporous silica composites with carbon-functionalized pore-walls (denoted as MG@mSiO2-C composites) were synthesized and applied as restricted access matrix solid phase extraction (RAM-SPE) adsorbents for the determination of miglitol and voglibose in rat plasma by LC-MS/MS.
29501171	3	7	contain	have	657:660	arg1	nano-composites					626:640	The obtained nano-composites	613:640	The obtained nano-composites	613:640	The obtained nano-composites were proven to have many unique properties such as large specific surface area of 277.1 cm2 g-1, uniform mesopores with average pore size of 3.35 nm, and carbon-functionalized pore-walls.
29501171	3	7	contain	have	657:660	arg2	pore-walls					818:827	carbon-functionalized pore-walls	796:827	carbon-functionalized pore-walls	796:827	The obtained nano-composites were proven to have many unique properties such as large specific surface area of 277.1 cm2 g-1, uniform mesopores with average pore size of 3.35 nm, and carbon-functionalized pore-walls.
29501171	3	7	contain	have	657:660	arg2	properties					674:683	many unique properties	662:683	many unique properties such as large specific surface area of 277.1 cm2 g-1, uniform mesopores with average pore size of 3.35 nm, and carbon-functionalized pore-walls	662:827	The obtained nano-composites were proven to have many unique properties such as large specific surface area of 277.1 cm2 g-1, uniform mesopores with average pore size of 3.35 nm, and carbon-functionalized pore-walls.
29501171	3	7	contain	have	657:660	arg2	mesopores					747:755	uniform mesopores	739:755	uniform mesopores with average pore size of 3.35 nm	739:789	The obtained nano-composites were proven to have many unique properties such as large specific surface area of 277.1 cm2 g-1, uniform mesopores with average pore size of 3.35 nm, and carbon-functionalized pore-walls.
29501171	3	7	contain	have	657:660	arg2	area					716:719	large specific surface area	693:719	large specific surface area of 277.1 cm2 g-1	693:736	The obtained nano-composites were proven to have many unique properties such as large specific surface area of 277.1 cm2 g-1, uniform mesopores with average pore size of 3.35 nm, and carbon-functionalized pore-walls.
29501171	1	8	theme	miglitol	410:417	arg1	determination					393:405	the determination	389:405	the determination of miglitol and voglibose in rat plasma by LC-MS/MS	389:457	In this work, magnetic graphene/mesoporous silica composites with carbon-functionalized pore-walls (denoted as MG@mSiO2-C composites) were synthesized and applied as restricted access matrix solid phase extraction (RAM-SPE) adsorbents for the determination of miglitol and voglibose in rat plasma by LC-MS/MS.
29501171	6	9	theme	determination	1256:1268	arg1	R2					1284:1285	R2	1284:1285	R2	1284:1285	For both miglitol and voglibose, good linearities of 10-2000 ng mL-1 were obtained with determination coefficients (R2) > 0.99.
29501171	6	9	theme	determination	1256:1268	arg1	coefficients					1270:1281	determination coefficients	1256:1281	determination coefficients (R2) > 0.99	1256:1293	For both miglitol and voglibose, good linearities of 10-2000 ng mL-1 were obtained with determination coefficients (R2) > 0.99.
29501171	3	10	theme	3.35 nm	783:789	arg1	size					775:778	average pore size	762:778	average pore size of 3.35 nm	762:789	The obtained nano-composites were proven to have many unique properties such as large specific surface area of 277.1 cm2 g-1, uniform mesopores with average pore size of 3.35 nm, and carbon-functionalized pore-walls.
29501171	1	11	theme	voglibose	423:431	arg1	determination					393:405	the determination	389:405	the determination of miglitol and voglibose in rat plasma by LC-MS/MS	389:457	In this work, magnetic graphene/mesoporous silica composites with carbon-functionalized pore-walls (denoted as MG@mSiO2-C composites) were synthesized and applied as restricted access matrix solid phase extraction (RAM-SPE) adsorbents for the determination of miglitol and voglibose in rat plasma by LC-MS/MS.
29501171	2	12	theme	sulfuric	587:594	arg1	acid					596:599	sulfuric acid	587:599	sulfuric acid pretreated	587:610	The MG@mSiO2-C composites were synthesized by using the template (Cetyltrimethyl Ammonium Bromide, CTAB) as carbon source with sulfuric acid pretreated.
29501171	1	13	theme	rat	436:438	arg1	plasma					440:445	rat plasma	436:445	rat plasma	436:445	In this work, magnetic graphene/mesoporous silica composites with carbon-functionalized pore-walls (denoted as MG@mSiO2-C composites) were synthesized and applied as restricted access matrix solid phase extraction (RAM-SPE) adsorbents for the determination of miglitol and voglibose in rat plasma by LC-MS/MS.
29501171	9	14	from	determination	1583:1595	arg1	plasma					1627:1632	plasma	1627:1632	plasma	1627:1632	This MG@mSiO2-C composites-based RAM-SPE method offers high extraction efficiency for the determination of α-glucosidase inhibitor in plasma.
29501171	6	15	theme	10-2000 ng mL-1	1221:1235	arg1	linearities					1206:1216	good linearities	1201:1216	good linearities of 10-2000 ng mL-1	1201:1235	For both miglitol and voglibose, good linearities of 10-2000 ng mL-1 were obtained with determination coefficients (R2) > 0.99.
29501171	0	16	theme	selective	88:96	arg1	extraction					98:107	selective extraction	88:107	selective extraction of miglitol and voglibose in rat plasma	88:147	Preparation of carbon-functionalized magnetic graphene/mesoporous silica composites for selective extraction of miglitol and voglibose in rat plasma.
29501171	3	17	with	pore-walls	818:827	arg1	size					775:778	average pore size	762:778	average pore size of 3.35 nm	762:789	The obtained nano-composites were proven to have many unique properties such as large specific surface area of 277.1 cm2 g-1, uniform mesopores with average pore size of 3.35 nm, and carbon-functionalized pore-walls.
29501171	1	18	from	plasma	440:445	arg1	determination					393:405	the determination	389:405	the determination of miglitol and voglibose in rat plasma by LC-MS/MS	389:457	In this work, magnetic graphene/mesoporous silica composites with carbon-functionalized pore-walls (denoted as MG@mSiO2-C composites) were synthesized and applied as restricted access matrix solid phase extraction (RAM-SPE) adsorbents for the determination of miglitol and voglibose in rat plasma by LC-MS/MS.
29501171	1	19	theme	graphene/mesoporous	173:191	arg1	adsorbents					374:383	restricted access matrix solid phase extraction (RAM-SPE) adsorbents	316:383	restricted access matrix solid phase extraction (RAM-SPE) adsorbents for the determination of miglitol and voglibose in rat plasma by LC-MS/MS	316:457	In this work, magnetic graphene/mesoporous silica composites with carbon-functionalized pore-walls (denoted as MG@mSiO2-C composites) were synthesized and applied as restricted access matrix solid phase extraction (RAM-SPE) adsorbents for the determination of miglitol and voglibose in rat plasma by LC-MS/MS.
29501171	1	19	theme	graphene/mesoporous	173:191	arg1	composites					200:209	magnetic graphene/mesoporous silica composites	164:209	magnetic graphene/mesoporous silica composites with carbon-functionalized pore-walls (denoted as MG@mSiO2-C composites)	164:282	In this work, magnetic graphene/mesoporous silica composites with carbon-functionalized pore-walls (denoted as MG@mSiO2-C composites) were synthesized and applied as restricted access matrix solid phase extraction (RAM-SPE) adsorbents for the determination of miglitol and voglibose in rat plasma by LC-MS/MS.
29501171	4	20	theme	rat	989:991	arg1	plasma					993:998	rat plasma	989:998	rat plasma	989:998	Taking advantage of the hydrophilic interaction between carbon and glycans, α-glucosidase inhibitors (miglitol and voglibose) could be directly extracted from rat plasma with no need of other pre-treatment procedures.
29501171	5	21	theme	adsorption	1119:1128	arg1	time					1130:1133	adsorption time	1119:1133	adsorption time	1119:1133	The SPE conditions such as the adsorbent amount, elution solvent type, adsorption time and elution time were optimized.
29501171	3	22	with	mesopores	747:755	arg1	size					775:778	average pore size	762:778	average pore size of 3.35 nm	762:789	The obtained nano-composites were proven to have many unique properties such as large specific surface area of 277.1 cm2 g-1, uniform mesopores with average pore size of 3.35 nm, and carbon-functionalized pore-walls.
29501171	2	23	theme	@	466:466	arg1	composites					475:484	The MG@mSiO2-C composites	460:484	The MG@mSiO2-C composites	460:484	The MG@mSiO2-C composites were synthesized by using the template (Cetyltrimethyl Ammonium Bromide, CTAB) as carbon source with sulfuric acid pretreated.
29501171	9	24	theme	MG	1498:1499	arg1	method					1534:1539	This MG@mSiO2-C composites-based RAM-SPE method	1493:1539	This MG@mSiO2-C composites-based RAM-SPE method	1493:1539	This MG@mSiO2-C composites-based RAM-SPE method offers high extraction efficiency for the determination of α-glucosidase inhibitor in plasma.
29501171	0	25	theme	carbon-functionalized	15:35	arg1	composites					73:82	carbon-functionalized magnetic graphene/mesoporous silica composites	15:82	carbon-functionalized magnetic graphene/mesoporous silica composites	15:82	Preparation of carbon-functionalized magnetic graphene/mesoporous silica composites for selective extraction of miglitol and voglibose in rat plasma.
29501171	1	26	theme	restricted	316:325	arg1	RAM-SPE					365:371	RAM-SPE	365:371	RAM-SPE	365:371	In this work, magnetic graphene/mesoporous silica composites with carbon-functionalized pore-walls (denoted as MG@mSiO2-C composites) were synthesized and applied as restricted access matrix solid phase extraction (RAM-SPE) adsorbents for the determination of miglitol and voglibose in rat plasma by LC-MS/MS.
29501171	1	26	theme	restricted	316:325	arg1	extraction					353:362	restricted access matrix solid phase extraction	316:362	restricted access matrix solid phase extraction (RAM-SPE) adsorbents for the determination of miglitol and voglibose in rat plasma by LC-MS/MS	316:457	In this work, magnetic graphene/mesoporous silica composites with carbon-functionalized pore-walls (denoted as MG@mSiO2-C composites) were synthesized and applied as restricted access matrix solid phase extraction (RAM-SPE) adsorbents for the determination of miglitol and voglibose in rat plasma by LC-MS/MS.
29501171	9	27	theme	mSiO2-C	1501:1507	arg1	method					1534:1539	This MG@mSiO2-C composites-based RAM-SPE method	1493:1539	This MG@mSiO2-C composites-based RAM-SPE method	1493:1539	This MG@mSiO2-C composites-based RAM-SPE method offers high extraction efficiency for the determination of α-glucosidase inhibitor in plasma.
29501171	4	28	theme	other	1016:1020	arg1	procedures					1036:1045	other pre-treatment procedures	1016:1045	other pre-treatment procedures	1016:1045	Taking advantage of the hydrophilic interaction between carbon and glycans, α-glucosidase inhibitors (miglitol and voglibose) could be directly extracted from rat plasma with no need of other pre-treatment procedures.
29501171	3	29	theme	unique	667:672	arg1	area					716:719	large specific surface area	693:719	large specific surface area of 277.1 cm2 g-1	693:736	The obtained nano-composites were proven to have many unique properties such as large specific surface area of 277.1 cm2 g-1, uniform mesopores with average pore size of 3.35 nm, and carbon-functionalized pore-walls.
29501171	3	29	theme	unique	667:672	arg1	pore-walls					818:827	carbon-functionalized pore-walls	796:827	carbon-functionalized pore-walls	796:827	The obtained nano-composites were proven to have many unique properties such as large specific surface area of 277.1 cm2 g-1, uniform mesopores with average pore size of 3.35 nm, and carbon-functionalized pore-walls.
29501171	3	29	theme	unique	667:672	arg1	mesopores					747:755	uniform mesopores	739:755	uniform mesopores with average pore size of 3.35 nm	739:789	The obtained nano-composites were proven to have many unique properties such as large specific surface area of 277.1 cm2 g-1, uniform mesopores with average pore size of 3.35 nm, and carbon-functionalized pore-walls.
29501171	3	29	theme	unique	667:672	arg1	properties					674:683	many unique properties	662:683	many unique properties such as large specific surface area of 277.1 cm2 g-1, uniform mesopores with average pore size of 3.35 nm, and carbon-functionalized pore-walls	662:827	The obtained nano-composites were proven to have many unique properties such as large specific surface area of 277.1 cm2 g-1, uniform mesopores with average pore size of 3.35 nm, and carbon-functionalized pore-walls.
29501171	0	30	theme	graphene/mesoporous	46:64	arg1	composites					73:82	carbon-functionalized magnetic graphene/mesoporous silica composites	15:82	carbon-functionalized magnetic graphene/mesoporous silica composites	15:82	Preparation of carbon-functionalized magnetic graphene/mesoporous silica composites for selective extraction of miglitol and voglibose in rat plasma.
29501171	1	31	theme	matrix	334:339	arg1	RAM-SPE					365:371	RAM-SPE	365:371	RAM-SPE	365:371	In this work, magnetic graphene/mesoporous silica composites with carbon-functionalized pore-walls (denoted as MG@mSiO2-C composites) were synthesized and applied as restricted access matrix solid phase extraction (RAM-SPE) adsorbents for the determination of miglitol and voglibose in rat plasma by LC-MS/MS.
29501171	1	31	theme	matrix	334:339	arg1	extraction					353:362	restricted access matrix solid phase extraction	316:362	restricted access matrix solid phase extraction (RAM-SPE) adsorbents for the determination of miglitol and voglibose in rat plasma by LC-MS/MS	316:457	In this work, magnetic graphene/mesoporous silica composites with carbon-functionalized pore-walls (denoted as MG@mSiO2-C composites) were synthesized and applied as restricted access matrix solid phase extraction (RAM-SPE) adsorbents for the determination of miglitol and voglibose in rat plasma by LC-MS/MS.
29501171	9	32	theme	RAM-SPE	1526:1532	arg1	method					1534:1539	This MG@mSiO2-C composites-based RAM-SPE method	1493:1539	This MG@mSiO2-C composites-based RAM-SPE method	1493:1539	This MG@mSiO2-C composites-based RAM-SPE method offers high extraction efficiency for the determination of α-glucosidase inhibitor in plasma.
29501171	4	33	theme	procedures	1036:1045	arg1	need					1008:1011	no need	1005:1011	no need of other pre-treatment procedures	1005:1045	Taking advantage of the hydrophilic interaction between carbon and glycans, α-glucosidase inhibitors (miglitol and voglibose) could be directly extracted from rat plasma with no need of other pre-treatment procedures.
29501171	0	34	theme	composites	73:82	arg1	Preparation					0:10	Preparation	0:10	Preparation of carbon-functionalized magnetic graphene/mesoporous silica composites for selective extraction of miglitol and voglibose in rat plasma.	0:148	Preparation of carbon-functionalized magnetic graphene/mesoporous silica composites for selective extraction of miglitol and voglibose in rat plasma.
29501171	1	35	theme	phase	347:351	arg1	RAM-SPE					365:371	RAM-SPE	365:371	RAM-SPE	365:371	In this work, magnetic graphene/mesoporous silica composites with carbon-functionalized pore-walls (denoted as MG@mSiO2-C composites) were synthesized and applied as restricted access matrix solid phase extraction (RAM-SPE) adsorbents for the determination of miglitol and voglibose in rat plasma by LC-MS/MS.
29501171	1	35	theme	phase	347:351	arg1	extraction					353:362	restricted access matrix solid phase extraction	316:362	restricted access matrix solid phase extraction (RAM-SPE) adsorbents for the determination of miglitol and voglibose in rat plasma by LC-MS/MS	316:457	In this work, magnetic graphene/mesoporous silica composites with carbon-functionalized pore-walls (denoted as MG@mSiO2-C composites) were synthesized and applied as restricted access matrix solid phase extraction (RAM-SPE) adsorbents for the determination of miglitol and voglibose in rat plasma by LC-MS/MS.
29501171	6	36	theme	good	1201:1204	arg1	linearities					1206:1216	good linearities	1201:1216	good linearities of 10-2000 ng mL-1	1201:1235	For both miglitol and voglibose, good linearities of 10-2000 ng mL-1 were obtained with determination coefficients (R2) > 0.99.
29501171	3	37	theme	large	693:697	arg1	area					716:719	large specific surface area	693:719	large specific surface area of 277.1 cm2 g-1	693:736	The obtained nano-composites were proven to have many unique properties such as large specific surface area of 277.1 cm2 g-1, uniform mesopores with average pore size of 3.35 nm, and carbon-functionalized pore-walls.
29501171	1	38	theme	MG	261:262	arg1	composites					272:281	MG@mSiO2-C composites	261:281	MG@mSiO2-C composites	261:281	In this work, magnetic graphene/mesoporous silica composites with carbon-functionalized pore-walls (denoted as MG@mSiO2-C composites) were synthesized and applied as restricted access matrix solid phase extraction (RAM-SPE) adsorbents for the determination of miglitol and voglibose in rat plasma by LC-MS/MS.
29501171	1	39	with	composites	200:209	arg1	pore-walls					238:247	carbon-functionalized pore-walls	216:247	carbon-functionalized pore-walls (denoted as MG@mSiO2-C composites)	216:282	In this work, magnetic graphene/mesoporous silica composites with carbon-functionalized pore-walls (denoted as MG@mSiO2-C composites) were synthesized and applied as restricted access matrix solid phase extraction (RAM-SPE) adsorbents for the determination of miglitol and voglibose in rat plasma by LC-MS/MS.
29501171	9	40	theme	extraction	1553:1562	arg1	efficiency					1564:1573	high extraction efficiency	1548:1573	high extraction efficiency for the determination of α-glucosidase inhibitor in plasma	1548:1632	This MG@mSiO2-C composites-based RAM-SPE method offers high extraction efficiency for the determination of α-glucosidase inhibitor in plasma.
29501171	1	41	dep	pore-walls	238:247	arg1	denoted					250:256	denoted	250:256	denoted as MG@mSiO2-C composites	250:281	In this work, magnetic graphene/mesoporous silica composites with carbon-functionalized pore-walls (denoted as MG@mSiO2-C composites) were synthesized and applied as restricted access matrix solid phase extraction (RAM-SPE) adsorbents for the determination of miglitol and voglibose in rat plasma by LC-MS/MS.
29501171	1	42	theme	mSiO2-C	264:270	arg1	composites					272:281	MG@mSiO2-C composites	261:281	MG@mSiO2-C composites	261:281	In this work, magnetic graphene/mesoporous silica composites with carbon-functionalized pore-walls (denoted as MG@mSiO2-C composites) were synthesized and applied as restricted access matrix solid phase extraction (RAM-SPE) adsorbents for the determination of miglitol and voglibose in rat plasma by LC-MS/MS.
29501171	3	43	theme	g-1	734:736	arg1	area					716:719	large specific surface area	693:719	large specific surface area of 277.1 cm2 g-1	693:736	The obtained nano-composites were proven to have many unique properties such as large specific surface area of 277.1 cm2 g-1, uniform mesopores with average pore size of 3.35 nm, and carbon-functionalized pore-walls.
29501171	3	43	theme	g-1	734:736	arg1	pore-walls					818:827	carbon-functionalized pore-walls	796:827	carbon-functionalized pore-walls	796:827	The obtained nano-composites were proven to have many unique properties such as large specific surface area of 277.1 cm2 g-1, uniform mesopores with average pore size of 3.35 nm, and carbon-functionalized pore-walls.
29501171	3	43	theme	g-1	734:736	arg1	mesopores					747:755	uniform mesopores	739:755	uniform mesopores with average pore size of 3.35 nm	739:789	The obtained nano-composites were proven to have many unique properties such as large specific surface area of 277.1 cm2 g-1, uniform mesopores with average pore size of 3.35 nm, and carbon-functionalized pore-walls.
29501171	3	44	theme	surface	708:714	arg1	area					716:719	large specific surface area	693:719	large specific surface area of 277.1 cm2 g-1	693:736	The obtained nano-composites were proven to have many unique properties such as large specific surface area of 277.1 cm2 g-1, uniform mesopores with average pore size of 3.35 nm, and carbon-functionalized pore-walls.
29501171	9	45	theme	α-glucosidase	1600:1612	arg1	inhibitor					1614:1622	α-glucosidase inhibitor	1600:1622	α-glucosidase inhibitor	1600:1622	This MG@mSiO2-C composites-based RAM-SPE method offers high extraction efficiency for the determination of α-glucosidase inhibitor in plasma.
29501171	3	46	theme	uniform	739:745	arg1	mesopores					747:755	uniform mesopores	739:755	uniform mesopores with average pore size of 3.35 nm	739:789	The obtained nano-composites were proven to have many unique properties such as large specific surface area of 277.1 cm2 g-1, uniform mesopores with average pore size of 3.35 nm, and carbon-functionalized pore-walls.
29501171	1	47	theme	silica	193:198	arg1	adsorbents					374:383	restricted access matrix solid phase extraction (RAM-SPE) adsorbents	316:383	restricted access matrix solid phase extraction (RAM-SPE) adsorbents for the determination of miglitol and voglibose in rat plasma by LC-MS/MS	316:457	In this work, magnetic graphene/mesoporous silica composites with carbon-functionalized pore-walls (denoted as MG@mSiO2-C composites) were synthesized and applied as restricted access matrix solid phase extraction (RAM-SPE) adsorbents for the determination of miglitol and voglibose in rat plasma by LC-MS/MS.
29501171	1	47	theme	silica	193:198	arg1	composites					200:209	magnetic graphene/mesoporous silica composites	164:209	magnetic graphene/mesoporous silica composites with carbon-functionalized pore-walls (denoted as MG@mSiO2-C composites)	164:282	In this work, magnetic graphene/mesoporous silica composites with carbon-functionalized pore-walls (denoted as MG@mSiO2-C composites) were synthesized and applied as restricted access matrix solid phase extraction (RAM-SPE) adsorbents for the determination of miglitol and voglibose in rat plasma by LC-MS/MS.
29501171	2	48	theme	mSiO2-C	467:473	arg1	composites					475:484	The MG@mSiO2-C composites	460:484	The MG@mSiO2-C composites	460:484	The MG@mSiO2-C composites were synthesized by using the template (Cetyltrimethyl Ammonium Bromide, CTAB) as carbon source with sulfuric acid pretreated.
29501171	4	49	theme	hydrophilic	854:864	arg1	interaction					866:876	the hydrophilic interaction	850:876	the hydrophilic interaction between carbon and glycans	850:903	Taking advantage of the hydrophilic interaction between carbon and glycans, α-glucosidase inhibitors (miglitol and voglibose) could be directly extracted from rat plasma with no need of other pre-treatment procedures.
29501171	4	50	dep	inhibitors	920:929	arg1	miglitol					932:939	miglitol	932:939	miglitol	932:939	Taking advantage of the hydrophilic interaction between carbon and glycans, α-glucosidase inhibitors (miglitol and voglibose) could be directly extracted from rat plasma with no need of other pre-treatment procedures.
29501171	4	50	dep	inhibitors	920:929	arg1	inhibitors					920:929	α-glucosidase inhibitors	906:929	α-glucosidase inhibitors (miglitol and voglibose)	906:954	Taking advantage of the hydrophilic interaction between carbon and glycans, α-glucosidase inhibitors (miglitol and voglibose) could be directly extracted from rat plasma with no need of other pre-treatment procedures.
29501171	4	50	dep	inhibitors	920:929	arg1	voglibose					945:953	voglibose	945:953	voglibose	945:953	Taking advantage of the hydrophilic interaction between carbon and glycans, α-glucosidase inhibitors (miglitol and voglibose) could be directly extracted from rat plasma with no need of other pre-treatment procedures.
29501171	3	51	theme	pore	770:773	arg1	size					775:778	average pore size	762:778	average pore size of 3.35 nm	762:789	The obtained nano-composites were proven to have many unique properties such as large specific surface area of 277.1 cm2 g-1, uniform mesopores with average pore size of 3.35 nm, and carbon-functionalized pore-walls.
29501171	7	52	theme	=	1367:1367	arg1	n					1365:1365	n = 6	1365:1369	n = 6	1365:1369	The intra-day and inter-day RSDs were 3.3-6.9% (n = 6) and 6.0-8.0% (n = 6), respectively.
29501171	7	52	theme	=	1367:1367	arg1	%					1362:1362	6.0-8.0%	1355:1362	6.0-8.0% (n = 6)	1355:1370	The intra-day and inter-day RSDs were 3.3-6.9% (n = 6) and 6.0-8.0% (n = 6), respectively.
29501171	0	53	from	extraction	98:107	arg1	plasma					142:147	rat plasma	138:147	rat plasma	138:147	Preparation of carbon-functionalized magnetic graphene/mesoporous silica composites for selective extraction of miglitol and voglibose in rat plasma.
29501171	1	54	from	voglibose	423:431	arg1	plasma					440:445	rat plasma	436:445	rat plasma	436:445	In this work, magnetic graphene/mesoporous silica composites with carbon-functionalized pore-walls (denoted as MG@mSiO2-C composites) were synthesized and applied as restricted access matrix solid phase extraction (RAM-SPE) adsorbents for the determination of miglitol and voglibose in rat plasma by LC-MS/MS.
29501171	5	55	theme	solvent	1105:1111	arg1	type					1113:1116	elution solvent type	1097:1116	elution solvent type	1097:1116	The SPE conditions such as the adsorbent amount, elution solvent type, adsorption time and elution time were optimized.
29501171	2	56	theme	carbon	568:573	arg1	source					575:580	carbon source	568:580	carbon source with sulfuric acid pretreated	568:610	The MG@mSiO2-C composites were synthesized by using the template (Cetyltrimethyl Ammonium Bromide, CTAB) as carbon source with sulfuric acid pretreated.
29501171	8	57	theme	%	1433:1433	arg1	range					1414:1418	the range	1410:1418	the range of 99.9-100.4%	1410:1433	The recoveries were in the range of 99.9-100.4% and the sensitivities were as low as 2-2.5 ng mL-1 (LOD).
29501171	2	58	theme	Cetyltrimethyl	526:539	arg1	CTAB					559:562	CTAB	559:562	CTAB	559:562	The MG@mSiO2-C composites were synthesized by using the template (Cetyltrimethyl Ammonium Bromide, CTAB) as carbon source with sulfuric acid pretreated.
29501171	2	58	theme	Cetyltrimethyl	526:539	arg1	Bromide					550:556	Cetyltrimethyl Ammonium Bromide	526:556	Cetyltrimethyl Ammonium Bromide	526:556	The MG@mSiO2-C composites were synthesized by using the template (Cetyltrimethyl Ammonium Bromide, CTAB) as carbon source with sulfuric acid pretreated.
29501171	5	59	theme	adsorbent	1079:1087	arg1	amount					1089:1094	the adsorbent amount	1075:1094	the adsorbent amount	1075:1094	The SPE conditions such as the adsorbent amount, elution solvent type, adsorption time and elution time were optimized.
29501171	3	60	theme	obtained	617:624	arg1	nano-composites					626:640	The obtained nano-composites	613:640	The obtained nano-composites	613:640	The obtained nano-composites were proven to have many unique properties such as large specific surface area of 277.1 cm2 g-1, uniform mesopores with average pore size of 3.35 nm, and carbon-functionalized pore-walls.
29501171	9	61	theme	inhibitor	1614:1622	arg1	determination					1583:1595	the determination	1579:1595	the determination of α-glucosidase inhibitor in plasma	1579:1632	This MG@mSiO2-C composites-based RAM-SPE method offers high extraction efficiency for the determination of α-glucosidase inhibitor in plasma.
29501171	7	62	theme	=	1346:1346	arg1	n					1344:1344	n = 6	1344:1348	n = 6	1344:1348	The intra-day and inter-day RSDs were 3.3-6.9% (n = 6) and 6.0-8.0% (n = 6), respectively.
29501171	7	62	theme	=	1346:1346	arg1	%					1341:1341	3.3-6.9%	1334:1341	3.3-6.9% (n = 6)	1334:1349	The intra-day and inter-day RSDs were 3.3-6.9% (n = 6) and 6.0-8.0% (n = 6), respectively.
29501171	3	63	theme	carbon-functionalized	796:816	arg1	pore-walls					818:827	carbon-functionalized pore-walls	796:827	carbon-functionalized pore-walls	796:827	The obtained nano-composites were proven to have many unique properties such as large specific surface area of 277.1 cm2 g-1, uniform mesopores with average pore size of 3.35 nm, and carbon-functionalized pore-walls.
29501171	0	64	theme	miglitol	112:119	arg1	extraction					98:107	selective extraction	88:107	selective extraction of miglitol and voglibose in rat plasma	88:147	Preparation of carbon-functionalized magnetic graphene/mesoporous silica composites for selective extraction of miglitol and voglibose in rat plasma.
29501171	5	65	theme	elution	1139:1145	arg1	time					1147:1150	elution time	1139:1150	elution time	1139:1150	The SPE conditions such as the adsorbent amount, elution solvent type, adsorption time and elution time were optimized.
29501171	1	66	theme	magnetic	164:171	arg1	adsorbents					374:383	restricted access matrix solid phase extraction (RAM-SPE) adsorbents	316:383	restricted access matrix solid phase extraction (RAM-SPE) adsorbents for the determination of miglitol and voglibose in rat plasma by LC-MS/MS	316:457	In this work, magnetic graphene/mesoporous silica composites with carbon-functionalized pore-walls (denoted as MG@mSiO2-C composites) were synthesized and applied as restricted access matrix solid phase extraction (RAM-SPE) adsorbents for the determination of miglitol and voglibose in rat plasma by LC-MS/MS.
29501171	1	66	theme	magnetic	164:171	arg1	composites					200:209	magnetic graphene/mesoporous silica composites	164:209	magnetic graphene/mesoporous silica composites with carbon-functionalized pore-walls (denoted as MG@mSiO2-C composites)	164:282	In this work, magnetic graphene/mesoporous silica composites with carbon-functionalized pore-walls (denoted as MG@mSiO2-C composites) were synthesized and applied as restricted access matrix solid phase extraction (RAM-SPE) adsorbents for the determination of miglitol and voglibose in rat plasma by LC-MS/MS.
29501171	0	67	theme	voglibose	125:133	arg1	extraction					98:107	selective extraction	88:107	selective extraction of miglitol and voglibose in rat plasma	88:147	Preparation of carbon-functionalized magnetic graphene/mesoporous silica composites for selective extraction of miglitol and voglibose in rat plasma.
29501171	2	68	theme	Ammonium	541:548	arg1	CTAB					559:562	CTAB	559:562	CTAB	559:562	The MG@mSiO2-C composites were synthesized by using the template (Cetyltrimethyl Ammonium Bromide, CTAB) as carbon source with sulfuric acid pretreated.
29501171	2	68	theme	Ammonium	541:548	arg1	Bromide					550:556	Cetyltrimethyl Ammonium Bromide	526:556	Cetyltrimethyl Ammonium Bromide	526:556	The MG@mSiO2-C composites were synthesized by using the template (Cetyltrimethyl Ammonium Bromide, CTAB) as carbon source with sulfuric acid pretreated.
29501171	0	69	theme	rat	138:140	arg1	plasma					142:147	rat plasma	138:147	rat plasma	138:147	Preparation of carbon-functionalized magnetic graphene/mesoporous silica composites for selective extraction of miglitol and voglibose in rat plasma.
29501171	1	70	from	miglitol	410:417	arg1	plasma					440:445	rat plasma	436:445	rat plasma	436:445	In this work, magnetic graphene/mesoporous silica composites with carbon-functionalized pore-walls (denoted as MG@mSiO2-C composites) were synthesized and applied as restricted access matrix solid phase extraction (RAM-SPE) adsorbents for the determination of miglitol and voglibose in rat plasma by LC-MS/MS.
29501171	1	71	from	determination	393:405	arg1	plasma					440:445	rat plasma	436:445	rat plasma	436:445	In this work, magnetic graphene/mesoporous silica composites with carbon-functionalized pore-walls (denoted as MG@mSiO2-C composites) were synthesized and applied as restricted access matrix solid phase extraction (RAM-SPE) adsorbents for the determination of miglitol and voglibose in rat plasma by LC-MS/MS.
29501171	2	72	theme	MG	464:465	arg1	composites					475:484	The MG@mSiO2-C composites	460:484	The MG@mSiO2-C composites	460:484	The MG@mSiO2-C composites were synthesized by using the template (Cetyltrimethyl Ammonium Bromide, CTAB) as carbon source with sulfuric acid pretreated.
29501171	0	73	theme	magnetic	37:44	arg1	composites					73:82	carbon-functionalized magnetic graphene/mesoporous silica composites	15:82	carbon-functionalized magnetic graphene/mesoporous silica composites	15:82	Preparation of carbon-functionalized magnetic graphene/mesoporous silica composites for selective extraction of miglitol and voglibose in rat plasma.
29501171	9	74	theme	@	1500:1500	arg1	method					1534:1539	This MG@mSiO2-C composites-based RAM-SPE method	1493:1539	This MG@mSiO2-C composites-based RAM-SPE method	1493:1539	This MG@mSiO2-C composites-based RAM-SPE method offers high extraction efficiency for the determination of α-glucosidase inhibitor in plasma.
29501171	7	75	theme	inter-day	1314:1322	arg1	RSDs					1324:1327	The intra-day and inter-day RSDs	1296:1327	The intra-day and inter-day RSDs	1296:1327	The intra-day and inter-day RSDs were 3.3-6.9% (n = 6) and 6.0-8.0% (n = 6), respectively.
29501171	7	75	theme	inter-day	1314:1322	arg1	%					1341:1341	3.3-6.9%	1334:1341	3.3-6.9% (n = 6)	1334:1349	The intra-day and inter-day RSDs were 3.3-6.9% (n = 6) and 6.0-8.0% (n = 6), respectively.
29501171	3	76	theme	many	662:665	arg1	area					716:719	large specific surface area	693:719	large specific surface area of 277.1 cm2 g-1	693:736	The obtained nano-composites were proven to have many unique properties such as large specific surface area of 277.1 cm2 g-1, uniform mesopores with average pore size of 3.35 nm, and carbon-functionalized pore-walls.
29501171	3	76	theme	many	662:665	arg1	pore-walls					818:827	carbon-functionalized pore-walls	796:827	carbon-functionalized pore-walls	796:827	The obtained nano-composites were proven to have many unique properties such as large specific surface area of 277.1 cm2 g-1, uniform mesopores with average pore size of 3.35 nm, and carbon-functionalized pore-walls.
29501171	3	76	theme	many	662:665	arg1	mesopores					747:755	uniform mesopores	739:755	uniform mesopores with average pore size of 3.35 nm	739:789	The obtained nano-composites were proven to have many unique properties such as large specific surface area of 277.1 cm2 g-1, uniform mesopores with average pore size of 3.35 nm, and carbon-functionalized pore-walls.
29501171	3	76	theme	many	662:665	arg1	properties					674:683	many unique properties	662:683	many unique properties such as large specific surface area of 277.1 cm2 g-1, uniform mesopores with average pore size of 3.35 nm, and carbon-functionalized pore-walls	662:827	The obtained nano-composites were proven to have many unique properties such as large specific surface area of 277.1 cm2 g-1, uniform mesopores with average pore size of 3.35 nm, and carbon-functionalized pore-walls.
29501171	0	77	theme	silica	66:71	arg1	composites					73:82	carbon-functionalized magnetic graphene/mesoporous silica composites	15:82	carbon-functionalized magnetic graphene/mesoporous silica composites	15:82	Preparation of carbon-functionalized magnetic graphene/mesoporous silica composites for selective extraction of miglitol and voglibose in rat plasma.
29501171	1	78	theme	access	327:332	arg1	RAM-SPE					365:371	RAM-SPE	365:371	RAM-SPE	365:371	In this work, magnetic graphene/mesoporous silica composites with carbon-functionalized pore-walls (denoted as MG@mSiO2-C composites) were synthesized and applied as restricted access matrix solid phase extraction (RAM-SPE) adsorbents for the determination of miglitol and voglibose in rat plasma by LC-MS/MS.
29501171	1	78	theme	access	327:332	arg1	extraction					353:362	restricted access matrix solid phase extraction	316:362	restricted access matrix solid phase extraction (RAM-SPE) adsorbents for the determination of miglitol and voglibose in rat plasma by LC-MS/MS	316:457	In this work, magnetic graphene/mesoporous silica composites with carbon-functionalized pore-walls (denoted as MG@mSiO2-C composites) were synthesized and applied as restricted access matrix solid phase extraction (RAM-SPE) adsorbents for the determination of miglitol and voglibose in rat plasma by LC-MS/MS.
29501171	9	79	theme	composites-based	1509:1524	arg1	method					1534:1539	This MG@mSiO2-C composites-based RAM-SPE method	1493:1539	This MG@mSiO2-C composites-based RAM-SPE method	1493:1539	This MG@mSiO2-C composites-based RAM-SPE method offers high extraction efficiency for the determination of α-glucosidase inhibitor in plasma.
29501171	7	80	theme	intra-day	1300:1308	arg1	RSDs					1324:1327	The intra-day and inter-day RSDs	1296:1327	The intra-day and inter-day RSDs	1296:1327	The intra-day and inter-day RSDs were 3.3-6.9% (n = 6) and 6.0-8.0% (n = 6), respectively.
29501171	7	80	theme	intra-day	1300:1308	arg1	%					1341:1341	3.3-6.9%	1334:1341	3.3-6.9% (n = 6)	1334:1349	The intra-day and inter-day RSDs were 3.3-6.9% (n = 6) and 6.0-8.0% (n = 6), respectively.
29501171	1	81	theme	solid	341:345	arg1	RAM-SPE					365:371	RAM-SPE	365:371	RAM-SPE	365:371	In this work, magnetic graphene/mesoporous silica composites with carbon-functionalized pore-walls (denoted as MG@mSiO2-C composites) were synthesized and applied as restricted access matrix solid phase extraction (RAM-SPE) adsorbents for the determination of miglitol and voglibose in rat plasma by LC-MS/MS.
29501171	1	81	theme	solid	341:345	arg1	extraction					353:362	restricted access matrix solid phase extraction	316:362	restricted access matrix solid phase extraction (RAM-SPE) adsorbents for the determination of miglitol and voglibose in rat plasma by LC-MS/MS	316:457	In this work, magnetic graphene/mesoporous silica composites with carbon-functionalized pore-walls (denoted as MG@mSiO2-C composites) were synthesized and applied as restricted access matrix solid phase extraction (RAM-SPE) adsorbents for the determination of miglitol and voglibose in rat plasma by LC-MS/MS.
29501171	5	82	theme	SPE	1052:1054	arg1	type					1113:1116	elution solvent type	1097:1116	elution solvent type	1097:1116	The SPE conditions such as the adsorbent amount, elution solvent type, adsorption time and elution time were optimized.
29501171	5	82	theme	SPE	1052:1054	arg1	time					1130:1133	adsorption time	1119:1133	adsorption time	1119:1133	The SPE conditions such as the adsorbent amount, elution solvent type, adsorption time and elution time were optimized.
29501171	5	82	theme	SPE	1052:1054	arg1	time					1147:1150	elution time	1139:1150	elution time	1139:1150	The SPE conditions such as the adsorbent amount, elution solvent type, adsorption time and elution time were optimized.
29501171	5	82	theme	SPE	1052:1054	arg1	amount					1089:1094	the adsorbent amount	1075:1094	the adsorbent amount	1075:1094	The SPE conditions such as the adsorbent amount, elution solvent type, adsorption time and elution time were optimized.
29501171	5	82	theme	SPE	1052:1054	arg1	conditions					1056:1065	The SPE conditions	1048:1065	The SPE conditions such as the adsorbent amount, elution solvent type, adsorption time and elution time	1048:1150	The SPE conditions such as the adsorbent amount, elution solvent type, adsorption time and elution time were optimized.
29501171	4	83	theme	pre-treatment	1022:1034	arg1	procedures					1036:1045	other pre-treatment procedures	1016:1045	other pre-treatment procedures	1016:1045	Taking advantage of the hydrophilic interaction between carbon and glycans, α-glucosidase inhibitors (miglitol and voglibose) could be directly extracted from rat plasma with no need of other pre-treatment procedures.
29501171	5	84	theme	elution	1097:1103	arg1	type					1113:1116	elution solvent type	1097:1116	elution solvent type	1097:1116	The SPE conditions such as the adsorbent amount, elution solvent type, adsorption time and elution time were optimized.
29501171	1	85	theme	extraction	353:362	arg1	adsorbents					374:383	restricted access matrix solid phase extraction (RAM-SPE) adsorbents	316:383	restricted access matrix solid phase extraction (RAM-SPE) adsorbents for the determination of miglitol and voglibose in rat plasma by LC-MS/MS	316:457	In this work, magnetic graphene/mesoporous silica composites with carbon-functionalized pore-walls (denoted as MG@mSiO2-C composites) were synthesized and applied as restricted access matrix solid phase extraction (RAM-SPE) adsorbents for the determination of miglitol and voglibose in rat plasma by LC-MS/MS.
29501171	1	85	theme	extraction	353:362	arg1	composites					200:209	magnetic graphene/mesoporous silica composites	164:209	magnetic graphene/mesoporous silica composites with carbon-functionalized pore-walls (denoted as MG@mSiO2-C composites)	164:282	In this work, magnetic graphene/mesoporous silica composites with carbon-functionalized pore-walls (denoted as MG@mSiO2-C composites) were synthesized and applied as restricted access matrix solid phase extraction (RAM-SPE) adsorbents for the determination of miglitol and voglibose in rat plasma by LC-MS/MS.
29501171	2	86	dep	template	516:523	arg1	CTAB					559:562	CTAB	559:562	CTAB	559:562	The MG@mSiO2-C composites were synthesized by using the template (Cetyltrimethyl Ammonium Bromide, CTAB) as carbon source with sulfuric acid pretreated.
29501171	2	86	dep	template	516:523	arg1	Bromide					550:556	Cetyltrimethyl Ammonium Bromide	526:556	Cetyltrimethyl Ammonium Bromide	526:556	The MG@mSiO2-C composites were synthesized by using the template (Cetyltrimethyl Ammonium Bromide, CTAB) as carbon source with sulfuric acid pretreated.
29501171	9	87	theme	high	1548:1551	arg1	efficiency					1564:1573	high extraction efficiency	1548:1573	high extraction efficiency for the determination of α-glucosidase inhibitor in plasma	1548:1632	This MG@mSiO2-C composites-based RAM-SPE method offers high extraction efficiency for the determination of α-glucosidase inhibitor in plasma.
29501171	1	88	theme	@	263:263	arg1	composites					272:281	MG@mSiO2-C composites	261:281	MG@mSiO2-C composites	261:281	In this work, magnetic graphene/mesoporous silica composites with carbon-functionalized pore-walls (denoted as MG@mSiO2-C composites) were synthesized and applied as restricted access matrix solid phase extraction (RAM-SPE) adsorbents for the determination of miglitol and voglibose in rat plasma by LC-MS/MS.
29501171	2	89	with	source	575:580	arg1	acid					596:599	sulfuric acid	587:599	sulfuric acid pretreated	587:610	The MG@mSiO2-C composites were synthesized by using the template (Cetyltrimethyl Ammonium Bromide, CTAB) as carbon source with sulfuric acid pretreated.
30791374	7	0	theme	AgNPs	1523:1527	arg1	concentration					1529:1541	the C. arvensis AgNPs concentration	1507:1541	the C. arvensis AgNPs concentration	1507:1541	Biogenic AgNPs obtained after a 24-h reaction period were used to engineer CHI-based conjugates and designated as CHI‒AgNPs1 to CHI‒AgNPs5, subject to the C. arvensis AgNPs concentration.
30791374	9	1	theme	Gram-	1660:1664	arg1	isolates					1676:1683	Gram+ and Gram- bacterial isolates	1650:1683	Gram+ and Gram- bacterial isolates	1650:1683	Gram+ and Gram- bacterial isolates, i.e., Staphylococcus aureus, and Escherichia coli, were used to test the antibacterial activities of newly developed CHI‒AgNPs conjugates.
30791374	9	1	theme	Gram-	1660:1664	arg1	coli					1731:1734	Escherichia coli	1719:1734	Escherichia coli	1719:1734	Gram+ and Gram- bacterial isolates, i.e., Staphylococcus aureus, and Escherichia coli, were used to test the antibacterial activities of newly developed CHI‒AgNPs conjugates.
30791374	9	1	theme	Gram-	1660:1664	arg1	i.e.					1686:1689	i.e.	1686:1689	i.e.	1686:1689	Gram+ and Gram- bacterial isolates, i.e., Staphylococcus aureus, and Escherichia coli, were used to test the antibacterial activities of newly developed CHI‒AgNPs conjugates.
30791374	9	1	theme	Gram-	1660:1664	arg1	aureus					1707:1712	Staphylococcus aureus	1692:1712	Staphylococcus aureus	1692:1712	Gram+ and Gram- bacterial isolates, i.e., Staphylococcus aureus, and Escherichia coli, were used to test the antibacterial activities of newly developed CHI‒AgNPs conjugates.
30791374	1	2	theme	major	279:283	arg1	threat					285:290	a major threat	277:290	a major threat to human safety	277:306	In the 21st century, with ever-increasing consciousness and social awareness, researchers must tackle the microbial infections that pose a major threat to human safety.
30791374	7	3	theme	C.	1511:1512	arg1	concentration					1529:1541	the C. arvensis AgNPs concentration	1507:1541	the C. arvensis AgNPs concentration	1507:1541	Biogenic AgNPs obtained after a 24-h reaction period were used to engineer CHI-based conjugates and designated as CHI‒AgNPs1 to CHI‒AgNPs5, subject to the C. arvensis AgNPs concentration.
30791374	1	4	theme	21st	147:150	arg1	century					152:158	the 21st century	143:158	the 21st century	143:158	In the 21st century, with ever-increasing consciousness and social awareness, researchers must tackle the microbial infections that pose a major threat to human safety.
30791374	11	5	theme	CHI‒AgNPs	2015:2023	arg1	conjugates					2025:2034	CHI‒AgNPs conjugates	2015:2034	CHI‒AgNPs conjugates	2015:2034	The antibiofilm potential of CHI‒AgNPs conjugates was tested against Pseudomonas aeruginosa.
30791374	13	6	theme	anticancer	2287:2296	arg1	potentialities					2298:2311	antibacterial, antibiofilm, and anticancer potentialities	2255:2311	antibacterial, antibiofilm, and anticancer potentialities	2255:2311	In summary, the newly engineered CHI‒AgNPs conjugates with antibacterial, antibiofilm, and anticancer potentialities are potential candidate materials for biomedical applications.
30791374	5	7	with	conjugates	862:871	arg1	antimicrobial					878:890	antimicrobial	878:890	antimicrobial	878:890	In this context, eco-friendly and novel conjugates with antimicrobial, antibiofilm, and anticancer potentialities were developed using biogenic silver nanoparticles (AgNPs) from Convolvulus arvensis (C. arvensis) extract and chitosan (CHI).
30791374	5	7	with	conjugates	862:871	arg1	potentialities					921:934	anticancer potentialities	910:934	anticancer potentialities	910:934	In this context, eco-friendly and novel conjugates with antimicrobial, antibiofilm, and anticancer potentialities were developed using biogenic silver nanoparticles (AgNPs) from Convolvulus arvensis (C. arvensis) extract and chitosan (CHI).
30791374	5	7	with	conjugates	862:871	arg1	antibiofilm					893:903	antibiofilm	893:903	antibiofilm	893:903	In this context, eco-friendly and novel conjugates with antimicrobial, antibiofilm, and anticancer potentialities were developed using biogenic silver nanoparticles (AgNPs) from Convolvulus arvensis (C. arvensis) extract and chitosan (CHI).
30791374	1	8	theme	social	200:205	arg1	awareness					207:215	social awareness	200:215	social awareness	200:215	In the 21st century, with ever-increasing consciousness and social awareness, researchers must tackle the microbial infections that pose a major threat to human safety.
30791374	13	9	theme	CHI‒AgNPs	2229:2237	arg1	conjugates					2239:2248	the newly engineered CHI‒AgNPs conjugates	2208:2248	the newly engineered CHI‒AgNPs conjugates with antibacterial, antibiofilm, and anticancer potentialities	2208:2311	In summary, the newly engineered CHI‒AgNPs conjugates with antibacterial, antibiofilm, and anticancer potentialities are potential candidate materials for biomedical applications.
30791374	13	9	theme	CHI‒AgNPs	2229:2237	arg1	materials					2337:2345	potential candidate materials	2317:2345	potential candidate materials for biomedical applications	2317:2373	In summary, the newly engineered CHI‒AgNPs conjugates with antibacterial, antibiofilm, and anticancer potentialities are potential candidate materials for biomedical applications.
30791374	10	10	dep	sample	1854:1859	arg1	comparison					1828:1837	comparison	1828:1837	comparison	1828:1837	In comparison to the control sample with bacterial cell count 1.5 × 10⁸ CFU/mL, a notable reduction in the log values was recorded for the CHI‒AgNPs3 conjugate.
30791374	10	11	from	reduction	1915:1923	arg1	values					1936:1941	the log values	1928:1941	the log values	1928:1941	In comparison to the control sample with bacterial cell count 1.5 × 10⁸ CFU/mL, a notable reduction in the log values was recorded for the CHI‒AgNPs3 conjugate.
30791374	13	12	with	conjugates	2239:2248	arg1	potentialities					2298:2311	antibacterial, antibiofilm, and anticancer potentialities	2255:2311	antibacterial, antibiofilm, and anticancer potentialities	2255:2311	In summary, the newly engineered CHI‒AgNPs conjugates with antibacterial, antibiofilm, and anticancer potentialities are potential candidate materials for biomedical applications.
30791374	2	13	theme	many	313:316	arg1	reasons					318:324	many reasons	313:324	many reasons	313:324	For many reasons, the emergence/re-emergence of threatening pathogens has increased and poses a serious challenge to health care services.
30791374	6	14	theme	FTIR	1123:1126	arg1	spectroscopy					1128:1139	UV-Vis and FTIR spectroscopy	1112:1139	spectroscopy	1128:1139	A range of instrumental and imaging tools, i.e., UV-Vis and FTIR spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy-dispersive spectroscopy (EDX), and X-ray diffraction (XRD), were employed to characterize the freshly extracted C. arvensis AgNPs.
30791374	13	15	theme	biomedical	2351:2360	arg1	applications					2362:2373	biomedical applications	2351:2373	biomedical applications	2351:2373	In summary, the newly engineered CHI‒AgNPs conjugates with antibacterial, antibiofilm, and anticancer potentialities are potential candidate materials for biomedical applications.
30791374	6	16	theme	arvensis	1340:1347	arg1	AgNPs					1349:1353	the freshly extracted C. arvensis AgNPs	1315:1353	the freshly extracted C. arvensis AgNPs	1315:1353	A range of instrumental and imaging tools, i.e., UV-Vis and FTIR spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy-dispersive spectroscopy (EDX), and X-ray diffraction (XRD), were employed to characterize the freshly extracted C. arvensis AgNPs.
30791374	3	17	dep	materials	578:586	arg1	engineer					591:598	engineer	591:598	to engineer antibacterial matrices	588:621	Considering the changing dynamics of 21st-century materials with medical potentialities, the integration of bioactive agents into materials to engineer antibacterial matrices has received limited attention so far.
30791374	7	18	theme	24-h	1388:1391	arg1	period					1402:1407	a 24-h reaction period	1386:1407	a 24-h reaction period	1386:1407	Biogenic AgNPs obtained after a 24-h reaction period were used to engineer CHI-based conjugates and designated as CHI‒AgNPs1 to CHI‒AgNPs5, subject to the C. arvensis AgNPs concentration.
30791374	6	19	theme	extracted	1327:1335	arg1	AgNPs					1349:1353	the freshly extracted C. arvensis AgNPs	1315:1353	the freshly extracted C. arvensis AgNPs	1315:1353	A range of instrumental and imaging tools, i.e., UV-Vis and FTIR spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy-dispersive spectroscopy (EDX), and X-ray diffraction (XRD), were employed to characterize the freshly extracted C. arvensis AgNPs.
30791374	10	20	theme	control	1846:1852	arg1	sample					1854:1859	the control sample	1842:1859	the control sample with bacterial cell count 1.5 × 10⁸ CFU/mL	1842:1902	In comparison to the control sample with bacterial cell count 1.5 × 10⁸ CFU/mL, a notable reduction in the log values was recorded for the CHI‒AgNPs3 conjugate.
30791374	13	21	theme	potential	2317:2325	arg1	conjugates					2239:2248	the newly engineered CHI‒AgNPs conjugates	2208:2248	the newly engineered CHI‒AgNPs conjugates with antibacterial, antibiofilm, and anticancer potentialities	2208:2311	In summary, the newly engineered CHI‒AgNPs conjugates with antibacterial, antibiofilm, and anticancer potentialities are potential candidate materials for biomedical applications.
30791374	13	21	theme	potential	2317:2325	arg1	materials					2337:2345	potential candidate materials	2317:2345	potential candidate materials for biomedical applications	2317:2373	In summary, the newly engineered CHI‒AgNPs conjugates with antibacterial, antibiofilm, and anticancer potentialities are potential candidate materials for biomedical applications.
30791374	6	22	theme	tools	1099:1103	arg1	range					1065:1069	A range	1063:1069	A range of instrumental and imaging tools, i.e., UV-Vis and FTIR spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy-dispersive spectroscopy (EDX), and X-ray diffraction (XRD),	1063:1283	A range of instrumental and imaging tools, i.e., UV-Vis and FTIR spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy-dispersive spectroscopy (EDX), and X-ray diffraction (XRD), were employed to characterize the freshly extracted C. arvensis AgNPs.
30791374	5	23	theme	eco-friendly	839:850	arg1	conjugates					862:871	eco-friendly and novel conjugates	839:871	eco-friendly and novel conjugates with antimicrobial, antibiofilm, and anticancer potentialities	839:934	In this context, eco-friendly and novel conjugates with antimicrobial, antibiofilm, and anticancer potentialities were developed using biogenic silver nanoparticles (AgNPs) from Convolvulus arvensis (C. arvensis) extract and chitosan (CHI).
30791374	13	24	theme	antibiofilm	2270:2280	arg1	potentialities					2298:2311	antibacterial, antibiofilm, and anticancer potentialities	2255:2311	antibacterial, antibiofilm, and anticancer potentialities	2255:2311	In summary, the newly engineered CHI‒AgNPs conjugates with antibacterial, antibiofilm, and anticancer potentialities are potential candidate materials for biomedical applications.
30791374	9	25	theme	antibacterial	1759:1771	arg1	activities					1773:1782	the antibacterial activities	1755:1782	the antibacterial activities of newly developed CHI‒AgNPs conjugates	1755:1822	Gram+ and Gram- bacterial isolates, i.e., Staphylococcus aureus, and Escherichia coli, were used to test the antibacterial activities of newly developed CHI‒AgNPs conjugates.
30791374	4	26	theme	microbial	771:779	arg1	contaminations					781:794	microbial contaminations	771:794	microbial contaminations in healthcare facilities	771:819	Thus, antimicrobial active conjugates are considered potential candidates to eradicate infections and reduce microbial contaminations in healthcare facilities.
30791374	5	27	theme	novel	856:860	arg1	conjugates					862:871	eco-friendly and novel conjugates	839:871	eco-friendly and novel conjugates with antimicrobial, antibiofilm, and anticancer potentialities	839:934	In this context, eco-friendly and novel conjugates with antimicrobial, antibiofilm, and anticancer potentialities were developed using biogenic silver nanoparticles (AgNPs) from Convolvulus arvensis (C. arvensis) extract and chitosan (CHI).
30791374	5	28	theme	Convolvulus	1000:1010	arg1	arvensis					1012:1019	Convolvulus arvensis	1000:1019	Convolvulus arvensis (C. arvensis) extract	1000:1041	In this context, eco-friendly and novel conjugates with antimicrobial, antibiofilm, and anticancer potentialities were developed using biogenic silver nanoparticles (AgNPs) from Convolvulus arvensis (C. arvensis) extract and chitosan (CHI).
30791374	5	28	theme	Convolvulus	1000:1010	arg1	arvensis					1025:1032	C. arvensis	1022:1032	C. arvensis	1022:1032	In this context, eco-friendly and novel conjugates with antimicrobial, antibiofilm, and anticancer potentialities were developed using biogenic silver nanoparticles (AgNPs) from Convolvulus arvensis (C. arvensis) extract and chitosan (CHI).
30791374	12	29	theme	CHI‒AgNPs3	2093:2102	arg1	conjugate					2104:2112	the CHI‒AgNPs3 conjugate	2089:2112	the CHI‒AgNPs3 conjugate	2089:2112	Moreover, the CHI‒AgNPs3 conjugate also showed substantial cytotoxicity against the MCF-7 (breast cancer) cell line.
30791374	4	30	theme	healthcare	799:808	arg1	facilities					810:819	healthcare facilities	799:819	healthcare facilities	799:819	Thus, antimicrobial active conjugates are considered potential candidates to eradicate infections and reduce microbial contaminations in healthcare facilities.
30791374	9	31	theme	developed	1793:1801	arg1	conjugates					1813:1822	newly developed CHI‒AgNPs conjugates	1787:1822	newly developed CHI‒AgNPs conjugates	1787:1822	Gram+ and Gram- bacterial isolates, i.e., Staphylococcus aureus, and Escherichia coli, were used to test the antibacterial activities of newly developed CHI‒AgNPs conjugates.
30791374	8	32	theme	stipulated	1554:1563	arg1	period					1573:1578	the stipulated loading period	1550:1578	the stipulated loading period	1550:1578	After the stipulated loading period, 92% loading efficiency (LE) was recorded for a CHI‒AgNPs3 conjugate.
30791374	3	33	theme	changing	464:471	arg1	dynamics					473:480	the changing dynamics	460:480	the changing dynamics of 21st-century materials with medical potentialities	460:534	Considering the changing dynamics of 21st-century materials with medical potentialities, the integration of bioactive agents into materials to engineer antibacterial matrices has received limited attention so far.
30791374	9	34	theme	conjugates	1813:1822	arg1	activities					1773:1782	the antibacterial activities	1755:1782	the antibacterial activities of newly developed CHI‒AgNPs conjugates	1755:1822	Gram+ and Gram- bacterial isolates, i.e., Staphylococcus aureus, and Escherichia coli, were used to test the antibacterial activities of newly developed CHI‒AgNPs conjugates.
30791374	6	35	dep	spectroscopy	1128:1139	arg1	i.e.					1106:1109	i.e.	1106:1109	i.e.	1106:1109	A range of instrumental and imaging tools, i.e., UV-Vis and FTIR spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy-dispersive spectroscopy (EDX), and X-ray diffraction (XRD), were employed to characterize the freshly extracted C. arvensis AgNPs.
30791374	2	36	theme	serious	405:411	arg1	challenge					413:421	a serious challenge	403:421	a serious challenge to health care services	403:445	For many reasons, the emergence/re-emergence of threatening pathogens has increased and poses a serious challenge to health care services.
30791374	0	37	theme	Biogenic	0:7	arg1	Conjugates					31:40	Biogenic Nanoparticle‒Chitosan Conjugates	0:40	Biogenic Nanoparticle‒Chitosan Conjugates with Antimicrobial, Antibiofilm, and Anticancer Potentialities: Development and Characterization.	0:138	Biogenic Nanoparticle‒Chitosan Conjugates with Antimicrobial, Antibiofilm, and Anticancer Potentialities: Development and Characterization.
30791374	0	37	theme	Biogenic	0:7	arg1	Characterization					122:137	Characterization	122:137	Characterization	122:137	Biogenic Nanoparticle‒Chitosan Conjugates with Antimicrobial, Antibiofilm, and Anticancer Potentialities: Development and Characterization.
30791374	0	37	theme	Biogenic	0:7	arg1	Development					106:116	Development	106:116	Development	106:116	Biogenic Nanoparticle‒Chitosan Conjugates with Antimicrobial, Antibiofilm, and Anticancer Potentialities: Development and Characterization.
30791374	1	38	theme	human	295:299	arg1	safety					301:306	human safety	295:306	human safety	295:306	In the 21st century, with ever-increasing consciousness and social awareness, researchers must tackle the microbial infections that pose a major threat to human safety.
30791374	7	39	theme	Biogenic	1356:1363	arg1	CHI‒AgNPs1					1470:1479	CHI‒AgNPs1	1470:1479	CHI‒AgNPs1 to CHI‒AgNPs5	1470:1493	Biogenic AgNPs obtained after a 24-h reaction period were used to engineer CHI-based conjugates and designated as CHI‒AgNPs1 to CHI‒AgNPs5, subject to the C. arvensis AgNPs concentration.
30791374	7	39	theme	Biogenic	1356:1363	arg1	AgNPs					1365:1369	Biogenic AgNPs	1356:1369	Biogenic AgNPs obtained after a 24-h reaction period	1356:1407	Biogenic AgNPs obtained after a 24-h reaction period were used to engineer CHI-based conjugates and designated as CHI‒AgNPs1 to CHI‒AgNPs5, subject to the C. arvensis AgNPs concentration.
30791374	8	40	theme	92	1581:1582	arg1	%					1583:1583	%	1583:1583	%	1583:1583	After the stipulated loading period, 92% loading efficiency (LE) was recorded for a CHI‒AgNPs3 conjugate.
30791374	3	41	theme	materials	498:506	arg1	dynamics					473:480	the changing dynamics	460:480	the changing dynamics of 21st-century materials with medical potentialities	460:534	Considering the changing dynamics of 21st-century materials with medical potentialities, the integration of bioactive agents into materials to engineer antibacterial matrices has received limited attention so far.
30791374	5	42	theme	anticancer	910:919	arg1	potentialities					921:934	anticancer potentialities	910:934	anticancer potentialities	910:934	In this context, eco-friendly and novel conjugates with antimicrobial, antibiofilm, and anticancer potentialities were developed using biogenic silver nanoparticles (AgNPs) from Convolvulus arvensis (C. arvensis) extract and chitosan (CHI).
30791374	6	43	theme	energy-dispersive	1218:1234	arg1	EDX					1250:1252	EDX	1250:1252	EDX	1250:1252	A range of instrumental and imaging tools, i.e., UV-Vis and FTIR spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy-dispersive spectroscopy (EDX), and X-ray diffraction (XRD), were employed to characterize the freshly extracted C. arvensis AgNPs.
30791374	6	43	theme	energy-dispersive	1218:1234	arg1	spectroscopy					1236:1247	energy-dispersive spectroscopy	1218:1247	energy-dispersive spectroscopy (EDX)	1218:1253	A range of instrumental and imaging tools, i.e., UV-Vis and FTIR spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy-dispersive spectroscopy (EDX), and X-ray diffraction (XRD), were employed to characterize the freshly extracted C. arvensis AgNPs.
30791374	6	44	theme	UV-Vis	1112:1117	arg1	spectroscopy					1128:1139	UV-Vis and FTIR spectroscopy	1112:1139	spectroscopy	1128:1139	A range of instrumental and imaging tools, i.e., UV-Vis and FTIR spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy-dispersive spectroscopy (EDX), and X-ray diffraction (XRD), were employed to characterize the freshly extracted C. arvensis AgNPs.
30791374	8	45	theme	loading	1585:1591	arg1	LE					1605:1606	LE	1605:1606	LE	1605:1606	After the stipulated loading period, 92% loading efficiency (LE) was recorded for a CHI‒AgNPs3 conjugate.
30791374	8	45	theme	loading	1585:1591	arg1	efficiency					1593:1602	92% loading efficiency	1581:1602	92% loading efficiency (LE)	1581:1607	After the stipulated loading period, 92% loading efficiency (LE) was recorded for a CHI‒AgNPs3 conjugate.
30791374	10	46	theme	cell	1876:1879	arg1	CFU/mL					1897:1902	bacterial cell count 1.5 × 10⁸ CFU/mL	1866:1902	bacterial cell count 1.5 × 10⁸ CFU/mL	1866:1902	In comparison to the control sample with bacterial cell count 1.5 × 10⁸ CFU/mL, a notable reduction in the log values was recorded for the CHI‒AgNPs3 conjugate.
30791374	2	47	theme	care	433:436	arg1	services					438:445	health care services	426:445	health care services	426:445	For many reasons, the emergence/re-emergence of threatening pathogens has increased and poses a serious challenge to health care services.
30791374	0	48	with	Conjugates	31:40	arg1	Potentialities					90:103	Anticancer Potentialities	79:103	Anticancer Potentialities	79:103	Biogenic Nanoparticle‒Chitosan Conjugates with Antimicrobial, Antibiofilm, and Anticancer Potentialities: Development and Characterization.
30791374	0	48	with	Conjugates	31:40	arg1	Antibiofilm					62:72	Antibiofilm	62:72	Antibiofilm	62:72	Biogenic Nanoparticle‒Chitosan Conjugates with Antimicrobial, Antibiofilm, and Anticancer Potentialities: Development and Characterization.
30791374	0	48	with	Conjugates	31:40	arg1	Antimicrobial					47:59	Antimicrobial	47:59	Antimicrobial	47:59	Biogenic Nanoparticle‒Chitosan Conjugates with Antimicrobial, Antibiofilm, and Anticancer Potentialities: Development and Characterization.
30791374	2	49	theme	pathogens	369:377	arg1	emergence/re-emergence					331:352	the emergence/re-emergence	327:352	the emergence/re-emergence of threatening pathogens	327:377	For many reasons, the emergence/re-emergence of threatening pathogens has increased and poses a serious challenge to health care services.
30791374	6	50	theme	transmission	1178:1189	arg1	TEM					1212:1214	TEM	1212:1214	TEM	1212:1214	A range of instrumental and imaging tools, i.e., UV-Vis and FTIR spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy-dispersive spectroscopy (EDX), and X-ray diffraction (XRD), were employed to characterize the freshly extracted C. arvensis AgNPs.
30791374	6	50	theme	transmission	1178:1189	arg1	microscopy					1200:1209	transmission electron microscopy	1178:1209	transmission electron microscopy (TEM)	1178:1215	A range of instrumental and imaging tools, i.e., UV-Vis and FTIR spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy-dispersive spectroscopy (EDX), and X-ray diffraction (XRD), were employed to characterize the freshly extracted C. arvensis AgNPs.
30791374	10	51	theme	10⁸	1893:1895	arg1	CFU/mL					1897:1902	bacterial cell count 1.5 × 10⁸ CFU/mL	1866:1902	bacterial cell count 1.5 × 10⁸ CFU/mL	1866:1902	In comparison to the control sample with bacterial cell count 1.5 × 10⁸ CFU/mL, a notable reduction in the log values was recorded for the CHI‒AgNPs3 conjugate.
30791374	12	52	theme	cancer	2177:2182	arg1	line					2190:2193	the MCF-7 (breast cancer) cell line	2159:2193	the MCF-7 (breast cancer) cell line	2159:2193	Moreover, the CHI‒AgNPs3 conjugate also showed substantial cytotoxicity against the MCF-7 (breast cancer) cell line.
30791374	13	53	from	materials	2337:2345	arg1	summary					2199:2205	summary	2199:2205	summary	2199:2205	In summary, the newly engineered CHI‒AgNPs conjugates with antibacterial, antibiofilm, and anticancer potentialities are potential candidate materials for biomedical applications.
30791374	3	54	theme	medical	513:519	arg1	potentialities					521:534	medical potentialities	513:534	medical potentialities	513:534	Considering the changing dynamics of 21st-century materials with medical potentialities, the integration of bioactive agents into materials to engineer antibacterial matrices has received limited attention so far.
30791374	5	55	from	chitosan	1047:1054	arg1	AgNPs					988:992	AgNPs	988:992	AgNPs	988:992	In this context, eco-friendly and novel conjugates with antimicrobial, antibiofilm, and anticancer potentialities were developed using biogenic silver nanoparticles (AgNPs) from Convolvulus arvensis (C. arvensis) extract and chitosan (CHI).
30791374	5	55	from	chitosan	1047:1054	arg1	nanoparticles					973:985	biogenic silver nanoparticles	957:985	biogenic silver nanoparticles (AgNPs) from Convolvulus arvensis (C. arvensis) extract and chitosan (CHI)	957:1060	In this context, eco-friendly and novel conjugates with antimicrobial, antibiofilm, and anticancer potentialities were developed using biogenic silver nanoparticles (AgNPs) from Convolvulus arvensis (C. arvensis) extract and chitosan (CHI).
30791374	1	56	theme	microbial	246:254	arg1	infections					256:265	the microbial infections	242:265	the microbial infections that pose a major threat to human safety	242:306	In the 21st century, with ever-increasing consciousness and social awareness, researchers must tackle the microbial infections that pose a major threat to human safety.
30791374	0	57	dep	Conjugates	31:40	arg1	Conjugates					31:40	Biogenic Nanoparticle‒Chitosan Conjugates	0:40	Biogenic Nanoparticle‒Chitosan Conjugates with Antimicrobial, Antibiofilm, and Anticancer Potentialities: Development and Characterization.	0:138	Biogenic Nanoparticle‒Chitosan Conjugates with Antimicrobial, Antibiofilm, and Anticancer Potentialities: Development and Characterization.
30791374	0	57	dep	Conjugates	31:40	arg1	Characterization					122:137	Characterization	122:137	Characterization	122:137	Biogenic Nanoparticle‒Chitosan Conjugates with Antimicrobial, Antibiofilm, and Anticancer Potentialities: Development and Characterization.
30791374	0	57	dep	Conjugates	31:40	arg1	Development					106:116	Development	106:116	Development	106:116	Biogenic Nanoparticle‒Chitosan Conjugates with Antimicrobial, Antibiofilm, and Anticancer Potentialities: Development and Characterization.
30791374	3	58	theme	21st-century	485:496	arg1	materials					498:506	21st-century materials	485:506	21st-century materials with medical potentialities	485:534	Considering the changing dynamics of 21st-century materials with medical potentialities, the integration of bioactive agents into materials to engineer antibacterial matrices has received limited attention so far.
30791374	12	59	theme	cell	2185:2188	arg1	line					2190:2193	the MCF-7 (breast cancer) cell line	2159:2193	the MCF-7 (breast cancer) cell line	2159:2193	Moreover, the CHI‒AgNPs3 conjugate also showed substantial cytotoxicity against the MCF-7 (breast cancer) cell line.
30791374	3	60	theme	bioactive	556:564	arg1	agents					566:571	bioactive agents	556:571	bioactive agents	556:571	Considering the changing dynamics of 21st-century materials with medical potentialities, the integration of bioactive agents into materials to engineer antibacterial matrices has received limited attention so far.
30791374	5	61	theme	silver	966:971	arg1	AgNPs					988:992	AgNPs	988:992	AgNPs	988:992	In this context, eco-friendly and novel conjugates with antimicrobial, antibiofilm, and anticancer potentialities were developed using biogenic silver nanoparticles (AgNPs) from Convolvulus arvensis (C. arvensis) extract and chitosan (CHI).
30791374	5	61	theme	silver	966:971	arg1	nanoparticles					973:985	biogenic silver nanoparticles	957:985	biogenic silver nanoparticles (AgNPs) from Convolvulus arvensis (C. arvensis) extract and chitosan (CHI)	957:1060	In this context, eco-friendly and novel conjugates with antimicrobial, antibiofilm, and anticancer potentialities were developed using biogenic silver nanoparticles (AgNPs) from Convolvulus arvensis (C. arvensis) extract and chitosan (CHI).
30791374	7	62	theme	arvensis	1514:1521	arg1	concentration					1529:1541	the C. arvensis AgNPs concentration	1507:1541	the C. arvensis AgNPs concentration	1507:1541	Biogenic AgNPs obtained after a 24-h reaction period were used to engineer CHI-based conjugates and designated as CHI‒AgNPs1 to CHI‒AgNPs5, subject to the C. arvensis AgNPs concentration.
30791374	10	63	theme	notable	1907:1913	arg1	reduction					1915:1923	a notable reduction	1905:1923	a notable reduction in the log values	1905:1941	In comparison to the control sample with bacterial cell count 1.5 × 10⁸ CFU/mL, a notable reduction in the log values was recorded for the CHI‒AgNPs3 conjugate.
30791374	9	64	theme	bacterial	1666:1674	arg1	isolates					1676:1683	Gram+ and Gram- bacterial isolates	1650:1683	Gram+ and Gram- bacterial isolates	1650:1683	Gram+ and Gram- bacterial isolates, i.e., Staphylococcus aureus, and Escherichia coli, were used to test the antibacterial activities of newly developed CHI‒AgNPs conjugates.
30791374	9	64	theme	bacterial	1666:1674	arg1	coli					1731:1734	Escherichia coli	1719:1734	Escherichia coli	1719:1734	Gram+ and Gram- bacterial isolates, i.e., Staphylococcus aureus, and Escherichia coli, were used to test the antibacterial activities of newly developed CHI‒AgNPs conjugates.
30791374	9	64	theme	bacterial	1666:1674	arg1	i.e.					1686:1689	i.e.	1686:1689	i.e.	1686:1689	Gram+ and Gram- bacterial isolates, i.e., Staphylococcus aureus, and Escherichia coli, were used to test the antibacterial activities of newly developed CHI‒AgNPs conjugates.
30791374	9	64	theme	bacterial	1666:1674	arg1	aureus					1707:1712	Staphylococcus aureus	1692:1712	Staphylococcus aureus	1692:1712	Gram+ and Gram- bacterial isolates, i.e., Staphylococcus aureus, and Escherichia coli, were used to test the antibacterial activities of newly developed CHI‒AgNPs conjugates.
30791374	6	65	theme	electron	1151:1158	arg1	SEM					1172:1174	SEM	1172:1174	SEM	1172:1174	A range of instrumental and imaging tools, i.e., UV-Vis and FTIR spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy-dispersive spectroscopy (EDX), and X-ray diffraction (XRD), were employed to characterize the freshly extracted C. arvensis AgNPs.
30791374	6	65	theme	electron	1151:1158	arg1	microscopy					1160:1169	scanning electron microscopy	1142:1169	scanning electron microscopy (SEM)	1142:1175	A range of instrumental and imaging tools, i.e., UV-Vis and FTIR spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy-dispersive spectroscopy (EDX), and X-ray diffraction (XRD), were employed to characterize the freshly extracted C. arvensis AgNPs.
30791374	10	66	theme	log	1932:1934	arg1	values					1936:1941	the log values	1928:1941	the log values	1928:1941	In comparison to the control sample with bacterial cell count 1.5 × 10⁸ CFU/mL, a notable reduction in the log values was recorded for the CHI‒AgNPs3 conjugate.
30791374	11	67	theme	antibiofilm	1990:2000	arg1	potential					2002:2010	The antibiofilm potential	1986:2010	The antibiofilm potential of CHI‒AgNPs conjugates	1986:2034	The antibiofilm potential of CHI‒AgNPs conjugates was tested against Pseudomonas aeruginosa.
30791374	5	68	theme	biogenic	957:964	arg1	AgNPs					988:992	AgNPs	988:992	AgNPs	988:992	In this context, eco-friendly and novel conjugates with antimicrobial, antibiofilm, and anticancer potentialities were developed using biogenic silver nanoparticles (AgNPs) from Convolvulus arvensis (C. arvensis) extract and chitosan (CHI).
30791374	5	68	theme	biogenic	957:964	arg1	nanoparticles					973:985	biogenic silver nanoparticles	957:985	biogenic silver nanoparticles (AgNPs) from Convolvulus arvensis (C. arvensis) extract and chitosan (CHI)	957:1060	In this context, eco-friendly and novel conjugates with antimicrobial, antibiofilm, and anticancer potentialities were developed using biogenic silver nanoparticles (AgNPs) from Convolvulus arvensis (C. arvensis) extract and chitosan (CHI).
30791374	4	69	theme	potential	715:723	arg1	candidates					725:734	potential candidates	715:734	potential candidates	715:734	Thus, antimicrobial active conjugates are considered potential candidates to eradicate infections and reduce microbial contaminations in healthcare facilities.
30791374	12	70	theme	MCF-7	2163:2167	arg1	line					2190:2193	the MCF-7 (breast cancer) cell line	2159:2193	the MCF-7 (breast cancer) cell line	2159:2193	Moreover, the CHI‒AgNPs3 conjugate also showed substantial cytotoxicity against the MCF-7 (breast cancer) cell line.
30791374	3	71	theme	antibacterial	600:612	arg1	matrices					614:621	antibacterial matrices	600:621	antibacterial matrices	600:621	Considering the changing dynamics of 21st-century materials with medical potentialities, the integration of bioactive agents into materials to engineer antibacterial matrices has received limited attention so far.
30791374	13	72	theme	candidate	2327:2335	arg1	conjugates					2239:2248	the newly engineered CHI‒AgNPs conjugates	2208:2248	the newly engineered CHI‒AgNPs conjugates with antibacterial, antibiofilm, and anticancer potentialities	2208:2311	In summary, the newly engineered CHI‒AgNPs conjugates with antibacterial, antibiofilm, and anticancer potentialities are potential candidate materials for biomedical applications.
30791374	13	72	theme	candidate	2327:2335	arg1	materials					2337:2345	potential candidate materials	2317:2345	potential candidate materials for biomedical applications	2317:2373	In summary, the newly engineered CHI‒AgNPs conjugates with antibacterial, antibiofilm, and anticancer potentialities are potential candidate materials for biomedical applications.
30791374	7	73	theme	CHI-based	1431:1439	arg1	conjugates					1441:1450	CHI-based conjugates	1431:1450	CHI-based conjugates	1431:1450	Biogenic AgNPs obtained after a 24-h reaction period were used to engineer CHI-based conjugates and designated as CHI‒AgNPs1 to CHI‒AgNPs5, subject to the C. arvensis AgNPs concentration.
30791374	4	74	theme	active	682:687	arg1	conjugates					689:698	antimicrobial active conjugates	668:698	antimicrobial active conjugates	668:698	Thus, antimicrobial active conjugates are considered potential candidates to eradicate infections and reduce microbial contaminations in healthcare facilities.
30791374	3	75	with	materials	498:506	arg1	potentialities					521:534	medical potentialities	513:534	medical potentialities	513:534	Considering the changing dynamics of 21st-century materials with medical potentialities, the integration of bioactive agents into materials to engineer antibacterial matrices has received limited attention so far.
30791374	12	76	theme	substantial	2126:2136	arg1	cytotoxicity					2138:2149	substantial cytotoxicity	2126:2149	substantial cytotoxicity against the MCF-7 (breast cancer) cell line	2126:2193	Moreover, the CHI‒AgNPs3 conjugate also showed substantial cytotoxicity against the MCF-7 (breast cancer) cell line.
30791374	3	77	theme	limited	636:642	arg1	attention					644:652	limited attention	636:652	limited attention	636:652	Considering the changing dynamics of 21st-century materials with medical potentialities, the integration of bioactive agents into materials to engineer antibacterial matrices has received limited attention so far.
30791374	5	78	theme	arvensis	1012:1019	arg1	extract					1035:1041	Convolvulus arvensis (C. arvensis) extract	1000:1041	Convolvulus arvensis (C. arvensis) extract	1000:1041	In this context, eco-friendly and novel conjugates with antimicrobial, antibiofilm, and anticancer potentialities were developed using biogenic silver nanoparticles (AgNPs) from Convolvulus arvensis (C. arvensis) extract and chitosan (CHI).
30791374	10	79	with	sample	1854:1859	arg1	CFU/mL					1897:1902	bacterial cell count 1.5 × 10⁸ CFU/mL	1866:1902	bacterial cell count 1.5 × 10⁸ CFU/mL	1866:1902	In comparison to the control sample with bacterial cell count 1.5 × 10⁸ CFU/mL, a notable reduction in the log values was recorded for the CHI‒AgNPs3 conjugate.
30791374	6	80	theme	X-ray	1260:1264	arg1	diffraction					1266:1276	X-ray diffraction	1260:1276	X-ray diffraction (XRD)	1260:1282	A range of instrumental and imaging tools, i.e., UV-Vis and FTIR spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy-dispersive spectroscopy (EDX), and X-ray diffraction (XRD), were employed to characterize the freshly extracted C. arvensis AgNPs.
30791374	6	80	theme	X-ray	1260:1264	arg1	XRD					1279:1281	XRD	1279:1281	XRD	1279:1281	A range of instrumental and imaging tools, i.e., UV-Vis and FTIR spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy-dispersive spectroscopy (EDX), and X-ray diffraction (XRD), were employed to characterize the freshly extracted C. arvensis AgNPs.
30791374	10	81	theme	CHI‒AgNPs3	1964:1973	arg1	conjugate					1975:1983	the CHI‒AgNPs3 conjugate	1960:1983	the CHI‒AgNPs3 conjugate	1960:1983	In comparison to the control sample with bacterial cell count 1.5 × 10⁸ CFU/mL, a notable reduction in the log values was recorded for the CHI‒AgNPs3 conjugate.
30791374	6	82	theme	C.	1337:1338	arg1	AgNPs					1349:1353	the freshly extracted C. arvensis AgNPs	1315:1353	the freshly extracted C. arvensis AgNPs	1315:1353	A range of instrumental and imaging tools, i.e., UV-Vis and FTIR spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy-dispersive spectroscopy (EDX), and X-ray diffraction (XRD), were employed to characterize the freshly extracted C. arvensis AgNPs.
30791374	5	83	theme	C.	1022:1023	arg1	arvensis					1012:1019	Convolvulus arvensis	1000:1019	Convolvulus arvensis (C. arvensis) extract	1000:1041	In this context, eco-friendly and novel conjugates with antimicrobial, antibiofilm, and anticancer potentialities were developed using biogenic silver nanoparticles (AgNPs) from Convolvulus arvensis (C. arvensis) extract and chitosan (CHI).
30791374	5	83	theme	C.	1022:1023	arg1	arvensis					1025:1032	C. arvensis	1022:1032	C. arvensis	1022:1032	In this context, eco-friendly and novel conjugates with antimicrobial, antibiofilm, and anticancer potentialities were developed using biogenic silver nanoparticles (AgNPs) from Convolvulus arvensis (C. arvensis) extract and chitosan (CHI).
30791374	11	84	theme	conjugates	2025:2034	arg1	potential					2002:2010	The antibiofilm potential	1986:2010	The antibiofilm potential of CHI‒AgNPs conjugates	1986:2034	The antibiofilm potential of CHI‒AgNPs conjugates was tested against Pseudomonas aeruginosa.
30791374	7	85	theme	reaction	1393:1400	arg1	period					1402:1407	a 24-h reaction period	1386:1407	a 24-h reaction period	1386:1407	Biogenic AgNPs obtained after a 24-h reaction period were used to engineer CHI-based conjugates and designated as CHI‒AgNPs1 to CHI‒AgNPs5, subject to the C. arvensis AgNPs concentration.
30791374	7	86	used	used	1414:1417	arg2	CHI‒AgNPs1					1470:1479	CHI‒AgNPs1	1470:1479	CHI‒AgNPs1 to CHI‒AgNPs5	1470:1493	Biogenic AgNPs obtained after a 24-h reaction period were used to engineer CHI-based conjugates and designated as CHI‒AgNPs1 to CHI‒AgNPs5, subject to the C. arvensis AgNPs concentration.
30791374	7	86	used	used	1414:1417	arg2	AgNPs					1365:1369	Biogenic AgNPs	1356:1369	Biogenic AgNPs obtained after a 24-h reaction period	1356:1407	Biogenic AgNPs obtained after a 24-h reaction period were used to engineer CHI-based conjugates and designated as CHI‒AgNPs1 to CHI‒AgNPs5, subject to the C. arvensis AgNPs concentration.
30791374	0	87	theme	Anticancer	79:88	arg1	Potentialities					90:103	Anticancer Potentialities	79:103	Anticancer Potentialities	79:103	Biogenic Nanoparticle‒Chitosan Conjugates with Antimicrobial, Antibiofilm, and Anticancer Potentialities: Development and Characterization.
30791374	6	88	theme	imaging	1091:1097	arg1	tools					1099:1103	instrumental and imaging tools	1074:1103	instrumental and imaging tools	1074:1103	A range of instrumental and imaging tools, i.e., UV-Vis and FTIR spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy-dispersive spectroscopy (EDX), and X-ray diffraction (XRD), were employed to characterize the freshly extracted C. arvensis AgNPs.
30791374	1	89	theme	ever-increasing	166:180	arg1	consciousness					182:194	ever-increasing consciousness	166:194	ever-increasing consciousness	166:194	In the 21st century, with ever-increasing consciousness and social awareness, researchers must tackle the microbial infections that pose a major threat to human safety.
30791374	6	90	theme	instrumental	1074:1085	arg1	tools					1099:1103	instrumental and imaging tools	1074:1103	instrumental and imaging tools	1074:1103	A range of instrumental and imaging tools, i.e., UV-Vis and FTIR spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy-dispersive spectroscopy (EDX), and X-ray diffraction (XRD), were employed to characterize the freshly extracted C. arvensis AgNPs.
30791374	8	91	theme	loading	1565:1571	arg1	period					1573:1578	the stipulated loading period	1550:1578	the stipulated loading period	1550:1578	After the stipulated loading period, 92% loading efficiency (LE) was recorded for a CHI‒AgNPs3 conjugate.
30791374	5	92	from	extract	1035:1041	arg1	AgNPs					988:992	AgNPs	988:992	AgNPs	988:992	In this context, eco-friendly and novel conjugates with antimicrobial, antibiofilm, and anticancer potentialities were developed using biogenic silver nanoparticles (AgNPs) from Convolvulus arvensis (C. arvensis) extract and chitosan (CHI).
30791374	5	92	from	extract	1035:1041	arg1	nanoparticles					973:985	biogenic silver nanoparticles	957:985	biogenic silver nanoparticles (AgNPs) from Convolvulus arvensis (C. arvensis) extract and chitosan (CHI)	957:1060	In this context, eco-friendly and novel conjugates with antimicrobial, antibiofilm, and anticancer potentialities were developed using biogenic silver nanoparticles (AgNPs) from Convolvulus arvensis (C. arvensis) extract and chitosan (CHI).
30791374	6	93	theme	scanning	1142:1149	arg1	SEM					1172:1174	SEM	1172:1174	SEM	1172:1174	A range of instrumental and imaging tools, i.e., UV-Vis and FTIR spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy-dispersive spectroscopy (EDX), and X-ray diffraction (XRD), were employed to characterize the freshly extracted C. arvensis AgNPs.
30791374	6	93	theme	scanning	1142:1149	arg1	microscopy					1160:1169	scanning electron microscopy	1142:1169	scanning electron microscopy (SEM)	1142:1175	A range of instrumental and imaging tools, i.e., UV-Vis and FTIR spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy-dispersive spectroscopy (EDX), and X-ray diffraction (XRD), were employed to characterize the freshly extracted C. arvensis AgNPs.
30791374	9	94	theme	CHI‒AgNPs	1803:1811	arg1	conjugates					1813:1822	newly developed CHI‒AgNPs conjugates	1787:1822	newly developed CHI‒AgNPs conjugates	1787:1822	Gram+ and Gram- bacterial isolates, i.e., Staphylococcus aureus, and Escherichia coli, were used to test the antibacterial activities of newly developed CHI‒AgNPs conjugates.
30791374	0	95	theme	Nanoparticle‒Chitosan	9:29	arg1	Conjugates					31:40	Biogenic Nanoparticle‒Chitosan Conjugates	0:40	Biogenic Nanoparticle‒Chitosan Conjugates with Antimicrobial, Antibiofilm, and Anticancer Potentialities: Development and Characterization.	0:138	Biogenic Nanoparticle‒Chitosan Conjugates with Antimicrobial, Antibiofilm, and Anticancer Potentialities: Development and Characterization.
30791374	0	95	theme	Nanoparticle‒Chitosan	9:29	arg1	Characterization					122:137	Characterization	122:137	Characterization	122:137	Biogenic Nanoparticle‒Chitosan Conjugates with Antimicrobial, Antibiofilm, and Anticancer Potentialities: Development and Characterization.
30791374	0	95	theme	Nanoparticle‒Chitosan	9:29	arg1	Development					106:116	Development	106:116	Development	106:116	Biogenic Nanoparticle‒Chitosan Conjugates with Antimicrobial, Antibiofilm, and Anticancer Potentialities: Development and Characterization.
30791374	8	96	theme	%	1583:1583	arg1	LE					1605:1606	LE	1605:1606	LE	1605:1606	After the stipulated loading period, 92% loading efficiency (LE) was recorded for a CHI‒AgNPs3 conjugate.
30791374	8	96	theme	%	1583:1583	arg1	efficiency					1593:1602	92% loading efficiency	1581:1602	92% loading efficiency (LE)	1581:1607	After the stipulated loading period, 92% loading efficiency (LE) was recorded for a CHI‒AgNPs3 conjugate.
30791374	10	97	theme	count	1881:1885	arg1	CFU/mL					1897:1902	bacterial cell count 1.5 × 10⁸ CFU/mL	1866:1902	bacterial cell count 1.5 × 10⁸ CFU/mL	1866:1902	In comparison to the control sample with bacterial cell count 1.5 × 10⁸ CFU/mL, a notable reduction in the log values was recorded for the CHI‒AgNPs3 conjugate.
30791374	13	98	theme	antibacterial	2255:2267	arg1	potentialities					2298:2311	antibacterial, antibiofilm, and anticancer potentialities	2255:2311	antibacterial, antibiofilm, and anticancer potentialities	2255:2311	In summary, the newly engineered CHI‒AgNPs conjugates with antibacterial, antibiofilm, and anticancer potentialities are potential candidate materials for biomedical applications.
30791374	9	99	used	used	1742:1745	arg2	aureus					1707:1712	Staphylococcus aureus	1692:1712	Staphylococcus aureus	1692:1712	Gram+ and Gram- bacterial isolates, i.e., Staphylococcus aureus, and Escherichia coli, were used to test the antibacterial activities of newly developed CHI‒AgNPs conjugates.
30791374	9	99	used	used	1742:1745	arg2	coli					1731:1734	Escherichia coli	1719:1734	Escherichia coli	1719:1734	Gram+ and Gram- bacterial isolates, i.e., Staphylococcus aureus, and Escherichia coli, were used to test the antibacterial activities of newly developed CHI‒AgNPs conjugates.
30791374	9	99	used	used	1742:1745	arg2	i.e.					1686:1689	i.e.	1686:1689	i.e.	1686:1689	Gram+ and Gram- bacterial isolates, i.e., Staphylococcus aureus, and Escherichia coli, were used to test the antibacterial activities of newly developed CHI‒AgNPs conjugates.
30791374	9	99	used	used	1742:1745	arg2	isolates					1676:1683	Gram+ and Gram- bacterial isolates	1650:1683	Gram+ and Gram- bacterial isolates	1650:1683	Gram+ and Gram- bacterial isolates, i.e., Staphylococcus aureus, and Escherichia coli, were used to test the antibacterial activities of newly developed CHI‒AgNPs conjugates.
30791374	4	100	from	contaminations	781:794	arg1	facilities					810:819	healthcare facilities	799:819	healthcare facilities	799:819	Thus, antimicrobial active conjugates are considered potential candidates to eradicate infections and reduce microbial contaminations in healthcare facilities.
30791374	10	101	theme	bacterial	1866:1874	arg1	CFU/mL					1897:1902	bacterial cell count 1.5 × 10⁸ CFU/mL	1866:1902	bacterial cell count 1.5 × 10⁸ CFU/mL	1866:1902	In comparison to the control sample with bacterial cell count 1.5 × 10⁸ CFU/mL, a notable reduction in the log values was recorded for the CHI‒AgNPs3 conjugate.
30791374	4	102	theme	antimicrobial	668:680	arg1	conjugates					689:698	antimicrobial active conjugates	668:698	antimicrobial active conjugates	668:698	Thus, antimicrobial active conjugates are considered potential candidates to eradicate infections and reduce microbial contaminations in healthcare facilities.
30791374	2	103	theme	health	426:431	arg1	services					438:445	health care services	426:445	health care services	426:445	For many reasons, the emergence/re-emergence of threatening pathogens has increased and poses a serious challenge to health care services.
30791374	6	104	theme	electron	1191:1198	arg1	TEM					1212:1214	TEM	1212:1214	TEM	1212:1214	A range of instrumental and imaging tools, i.e., UV-Vis and FTIR spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy-dispersive spectroscopy (EDX), and X-ray diffraction (XRD), were employed to characterize the freshly extracted C. arvensis AgNPs.
30791374	6	104	theme	electron	1191:1198	arg1	microscopy					1200:1209	transmission electron microscopy	1178:1209	transmission electron microscopy (TEM)	1178:1215	A range of instrumental and imaging tools, i.e., UV-Vis and FTIR spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy-dispersive spectroscopy (EDX), and X-ray diffraction (XRD), were employed to characterize the freshly extracted C. arvensis AgNPs.
30791374	2	105	theme	threatening	357:367	arg1	pathogens					369:377	threatening pathogens	357:377	threatening pathogens	357:377	For many reasons, the emergence/re-emergence of threatening pathogens has increased and poses a serious challenge to health care services.
30791374	13	106	theme	engineered	2218:2227	arg1	conjugates					2239:2248	the newly engineered CHI‒AgNPs conjugates	2208:2248	the newly engineered CHI‒AgNPs conjugates with antibacterial, antibiofilm, and anticancer potentialities	2208:2311	In summary, the newly engineered CHI‒AgNPs conjugates with antibacterial, antibiofilm, and anticancer potentialities are potential candidate materials for biomedical applications.
30791374	13	106	theme	engineered	2218:2227	arg1	materials					2337:2345	potential candidate materials	2317:2345	potential candidate materials for biomedical applications	2317:2373	In summary, the newly engineered CHI‒AgNPs conjugates with antibacterial, antibiofilm, and anticancer potentialities are potential candidate materials for biomedical applications.
30791374	10	107	theme	×	1891:1891	arg1	CFU/mL					1897:1902	bacterial cell count 1.5 × 10⁸ CFU/mL	1866:1902	bacterial cell count 1.5 × 10⁸ CFU/mL	1866:1902	In comparison to the control sample with bacterial cell count 1.5 × 10⁸ CFU/mL, a notable reduction in the log values was recorded for the CHI‒AgNPs3 conjugate.
30791374	12	108	theme	breast	2170:2175	arg1	line					2190:2193	the MCF-7 (breast cancer) cell line	2159:2193	the MCF-7 (breast cancer) cell line	2159:2193	Moreover, the CHI‒AgNPs3 conjugate also showed substantial cytotoxicity against the MCF-7 (breast cancer) cell line.
30791374	9	109	theme	Gram+	1650:1654	arg1	isolates					1676:1683	Gram+ and Gram- bacterial isolates	1650:1683	Gram+ and Gram- bacterial isolates	1650:1683	Gram+ and Gram- bacterial isolates, i.e., Staphylococcus aureus, and Escherichia coli, were used to test the antibacterial activities of newly developed CHI‒AgNPs conjugates.
30791374	9	109	theme	Gram+	1650:1654	arg1	coli					1731:1734	Escherichia coli	1719:1734	Escherichia coli	1719:1734	Gram+ and Gram- bacterial isolates, i.e., Staphylococcus aureus, and Escherichia coli, were used to test the antibacterial activities of newly developed CHI‒AgNPs conjugates.
30791374	9	109	theme	Gram+	1650:1654	arg1	i.e.					1686:1689	i.e.	1686:1689	i.e.	1686:1689	Gram+ and Gram- bacterial isolates, i.e., Staphylococcus aureus, and Escherichia coli, were used to test the antibacterial activities of newly developed CHI‒AgNPs conjugates.
30791374	9	109	theme	Gram+	1650:1654	arg1	aureus					1707:1712	Staphylococcus aureus	1692:1712	Staphylococcus aureus	1692:1712	Gram+ and Gram- bacterial isolates, i.e., Staphylococcus aureus, and Escherichia coli, were used to test the antibacterial activities of newly developed CHI‒AgNPs conjugates.
30791374	8	110	theme	CHI‒AgNPs3	1628:1637	arg1	conjugate					1639:1647	a CHI‒AgNPs3 conjugate	1626:1647	a CHI‒AgNPs3 conjugate	1626:1647	After the stipulated loading period, 92% loading efficiency (LE) was recorded for a CHI‒AgNPs3 conjugate.
30791374	3	111	theme	agents	566:571	arg1	integration					541:551	the integration	537:551	the integration of bioactive agents into materials to engineer antibacterial matrices	537:621	Considering the changing dynamics of 21st-century materials with medical potentialities, the integration of bioactive agents into materials to engineer antibacterial matrices has received limited attention so far.
30172244	7	0	theme	release	1264:1270	arg1	profiles					1272:1279	responsive release profiles	1253:1279	responsive release profiles	1253:1279	Moreover, the curcumin loaded hydrogel showed good antioxidant ability and pH responsive release profiles.
30172244	1	1	theme	great	289:293	arg1	significance					305:316	great practical significance	289:316	great practical significance	289:316	Designing wound dressing materials with outstanding therapeutic effects, self-healing, adhesiveness and suitable mechanical property has great practical significance in healthcare, especially for joints skin wound healing.
30172244	9	2	theme	self-healing	1609:1620	arg1	property					1642:1649	self-healing and good mechanical property	1609:1649	self-healing and good mechanical property	1609:1649	Taken together, the antibacterial adhesive hydrogels with self-healing and good mechanical property offer significant promise as dressing materials for joints skin wound healing.
30172244	8	3	theme	vascular	1470:1477	arg1	VEGF					1506:1509	VEGF	1506:1509	VEGF	1506:1509	In vivo experiments indicated that curcumin loaded hydrogels significantly accelerated wound healing rate with higher granulation tissue thickness and collagen disposition and upregulated vascular endothelial growth factor (VEGF) in a full-thickness skin defect model.
30172244	8	3	theme	vascular	1470:1477	arg1	factor					1498:1503	vascular endothelial growth factor	1470:1503	vascular endothelial growth factor (VEGF)	1470:1510	In vivo experiments indicated that curcumin loaded hydrogels significantly accelerated wound healing rate with higher granulation tissue thickness and collagen disposition and upregulated vascular endothelial growth factor (VEGF) in a full-thickness skin defect model.
30172244	8	4	theme	granulation	1400:1410	arg1	thickness					1419:1427	higher granulation tissue thickness	1393:1427	higher granulation tissue thickness	1393:1427	In vivo experiments indicated that curcumin loaded hydrogels significantly accelerated wound healing rate with higher granulation tissue thickness and collagen disposition and upregulated vascular endothelial growth factor (VEGF) in a full-thickness skin defect model.
30172244	9	5	theme	good	1626:1629	arg1	property					1642:1649	self-healing and good mechanical property	1609:1649	self-healing and good mechanical property	1609:1649	Taken together, the antibacterial adhesive hydrogels with self-healing and good mechanical property offer significant promise as dressing materials for joints skin wound healing.
30172244	7	6	theme	good	1221:1224	arg1	ability					1238:1244	good antioxidant ability	1221:1244	good antioxidant ability	1221:1244	Moreover, the curcumin loaded hydrogel showed good antioxidant ability and pH responsive release profiles.
30172244	8	7	theme	collagen	1433:1440	arg1	disposition					1442:1452	collagen disposition	1433:1452	collagen disposition	1433:1452	In vivo experiments indicated that curcumin loaded hydrogels significantly accelerated wound healing rate with higher granulation tissue thickness and collagen disposition and upregulated vascular endothelial growth factor (VEGF) in a full-thickness skin defect model.
30172244	0	8	theme	skin	132:135	arg1	healing					143:149	skin wound healing	132:149	skin wound healing	132:149	Antibacterial adhesive injectable hydrogels with rapid self-healing, extensibility and compressibility as wound dressing for joints skin wound healing.
30172244	9	9	theme	significant	1657:1667	arg1	promise					1669:1675	significant promise	1657:1675	significant promise	1657:1675	Taken together, the antibacterial adhesive hydrogels with self-healing and good mechanical property offer significant promise as dressing materials for joints skin wound healing.
30172244	9	9	theme	significant	1657:1667	arg1	materials					1689:1697	dressing materials	1680:1697	dressing materials for joints skin wound healing	1680:1727	Taken together, the antibacterial adhesive hydrogels with self-healing and good mechanical property offer significant promise as dressing materials for joints skin wound healing.
30172244	8	10	theme	healing	1375:1381	arg1	rate					1383:1386	wound healing rate	1369:1386	wound healing rate	1369:1386	In vivo experiments indicated that curcumin loaded hydrogels significantly accelerated wound healing rate with higher granulation tissue thickness and collagen disposition and upregulated vascular endothelial growth factor (VEGF) in a full-thickness skin defect model.
30172244	5	11	theme	suitable	944:951	arg1	stretchable					953:963	suitable stretchable	944:963	suitable stretchable	944:963	The hydrogel dressings showed suitable stretchable and compressive property, comparable modulus with human skin, good adhesiveness and fast self-healing ability to bear deformation.
30172244	4	12	theme	inherent	768:775	arg1	property					791:798	The inherent antibacterial property	764:798	The inherent antibacterial property	764:798	The inherent antibacterial property, pH-dependent biodegradation and release behavior were investigated to confirm multi-functions of wound dressing.
30172244	7	13	dep	ability	1238:1244	arg1	profiles					1272:1279	responsive release profiles	1253:1279	responsive release profiles	1253:1279	Moreover, the curcumin loaded hydrogel showed good antioxidant ability and pH responsive release profiles.
30172244	8	14	theme	growth	1491:1496	arg1	VEGF					1506:1509	VEGF	1506:1509	VEGF	1506:1509	In vivo experiments indicated that curcumin loaded hydrogels significantly accelerated wound healing rate with higher granulation tissue thickness and collagen disposition and upregulated vascular endothelial growth factor (VEGF) in a full-thickness skin defect model.
30172244	8	14	theme	growth	1491:1496	arg1	factor					1498:1503	vascular endothelial growth factor	1470:1503	vascular endothelial growth factor (VEGF)	1470:1510	In vivo experiments indicated that curcumin loaded hydrogels significantly accelerated wound healing rate with higher granulation tissue thickness and collagen disposition and upregulated vascular endothelial growth factor (VEGF) in a full-thickness skin defect model.
30172244	5	15	theme	good	1027:1030	arg1	adhesiveness					1032:1043	good adhesiveness	1027:1043	good adhesiveness	1027:1043	The hydrogel dressings showed suitable stretchable and compressive property, comparable modulus with human skin, good adhesiveness and fast self-healing ability to bear deformation.
30172244	9	16	theme	mechanical	1631:1640	arg1	property					1642:1649	self-healing and good mechanical property	1609:1649	self-healing and good mechanical property	1609:1649	Taken together, the antibacterial adhesive hydrogels with self-healing and good mechanical property offer significant promise as dressing materials for joints skin wound healing.
30172244	3	17	theme	quaternized	651:661	arg1	QCS					673:675	QCS	673:675	QCS	673:675	By combining the dynamic Schiff base and copolymer micelle cross-linking in one system, a series of hydrogels were prepared by mixing quaternized chitosan (QCS) and benzaldehyde-terminated Pluronic®F127 (PF127-CHO) under physiological conditions.
30172244	3	17	theme	quaternized	651:661	arg1	chitosan					663:670	quaternized chitosan	651:670	quaternized chitosan (QCS)	651:676	By combining the dynamic Schiff base and copolymer micelle cross-linking in one system, a series of hydrogels were prepared by mixing quaternized chitosan (QCS) and benzaldehyde-terminated Pluronic®F127 (PF127-CHO) under physiological conditions.
30172244	5	18	theme	comparable	991:1000	arg1	modulus					1002:1008	comparable modulus	991:1008	comparable modulus with human skin	991:1024	The hydrogel dressings showed suitable stretchable and compressive property, comparable modulus with human skin, good adhesiveness and fast self-healing ability to bear deformation.
30172244	0	19	dep	healing	143:149	arg1	hydrogels					34:42	Antibacterial adhesive injectable hydrogels	0:42	Antibacterial adhesive injectable hydrogels with rapid self-healing, extensibility and compressibility as wound dressing for joints	0:130	Antibacterial adhesive injectable hydrogels with rapid self-healing, extensibility and compressibility as wound dressing for joints skin wound healing.
30172244	5	20	with	modulus	1002:1008	arg1	skin					1021:1024	human skin	1015:1024	human skin	1015:1024	The hydrogel dressings showed suitable stretchable and compressive property, comparable modulus with human skin, good adhesiveness and fast self-healing ability to bear deformation.
30172244	0	21	theme	wound	106:110	arg1	dressing					112:119	wound dressing	106:119	wound dressing for joints	106:130	Antibacterial adhesive injectable hydrogels with rapid self-healing, extensibility and compressibility as wound dressing for joints skin wound healing.
30172244	4	22	theme	antibacterial	777:789	arg1	property					791:798	The inherent antibacterial property	764:798	The inherent antibacterial property	764:798	The inherent antibacterial property, pH-dependent biodegradation and release behavior were investigated to confirm multi-functions of wound dressing.
30172244	8	23	theme	skin	1532:1535	arg1	model					1544:1548	a full-thickness skin defect model	1515:1548	a full-thickness skin defect model	1515:1548	In vivo experiments indicated that curcumin loaded hydrogels significantly accelerated wound healing rate with higher granulation tissue thickness and collagen disposition and upregulated vascular endothelial growth factor (VEGF) in a full-thickness skin defect model.
30172244	2	24	theme	micelle/hydrogel	427:442	arg1	composites					444:453	self-healing injectable micelle/hydrogel composites	403:453	self-healing injectable micelle/hydrogel composites	403:453	Here, we designed a kind of self-healing injectable micelle/hydrogel composites with multi-functions as wound dressing for joint skin damage.
30172244	2	25	theme	joint	498:502	arg1	damage					509:514	joint skin damage	498:514	joint skin damage	498:514	Here, we designed a kind of self-healing injectable micelle/hydrogel composites with multi-functions as wound dressing for joint skin damage.
30172244	4	26	theme	pH-dependent	801:812	arg1	biodegradation					814:827	pH-dependent biodegradation	801:827	pH-dependent biodegradation	801:827	The inherent antibacterial property, pH-dependent biodegradation and release behavior were investigated to confirm multi-functions of wound dressing.
30172244	2	27	theme	self-healing	403:414	arg1	composites					444:453	self-healing injectable micelle/hydrogel composites	403:453	self-healing injectable micelle/hydrogel composites	403:453	Here, we designed a kind of self-healing injectable micelle/hydrogel composites with multi-functions as wound dressing for joint skin damage.
30172244	0	28	theme	Antibacterial	0:12	arg1	hydrogels					34:42	Antibacterial adhesive injectable hydrogels	0:42	Antibacterial adhesive injectable hydrogels with rapid self-healing, extensibility and compressibility as wound dressing for joints	0:130	Antibacterial adhesive injectable hydrogels with rapid self-healing, extensibility and compressibility as wound dressing for joints skin wound healing.
30172244	0	29	theme	injectable	23:32	arg1	hydrogels					34:42	Antibacterial adhesive injectable hydrogels	0:42	Antibacterial adhesive injectable hydrogels with rapid self-healing, extensibility and compressibility as wound dressing for joints	0:130	Antibacterial adhesive injectable hydrogels with rapid self-healing, extensibility and compressibility as wound dressing for joints skin wound healing.
30172244	5	30	theme	self-healing	1054:1065	arg1	ability					1067:1073	fast self-healing ability	1049:1073	fast self-healing ability to bear deformation	1049:1093	The hydrogel dressings showed suitable stretchable and compressive property, comparable modulus with human skin, good adhesiveness and fast self-healing ability to bear deformation.
30172244	3	31	theme	benzaldehyde-terminated	682:704	arg1	PF127-CHO					721:729	PF127-CHO	721:729	PF127-CHO	721:729	By combining the dynamic Schiff base and copolymer micelle cross-linking in one system, a series of hydrogels were prepared by mixing quaternized chitosan (QCS) and benzaldehyde-terminated Pluronic®F127 (PF127-CHO) under physiological conditions.
30172244	3	31	theme	benzaldehyde-terminated	682:704	arg1	Pluronic®F127					706:718	benzaldehyde-terminated Pluronic®F127	682:718	benzaldehyde-terminated Pluronic®F127 (PF127-CHO)	682:730	By combining the dynamic Schiff base and copolymer micelle cross-linking in one system, a series of hydrogels were prepared by mixing quaternized chitosan (QCS) and benzaldehyde-terminated Pluronic®F127 (PF127-CHO) under physiological conditions.
30172244	4	32	theme	dressing	904:911	arg1	multi-functions					879:893	multi-functions	879:893	multi-functions of wound dressing	879:911	The inherent antibacterial property, pH-dependent biodegradation and release behavior were investigated to confirm multi-functions of wound dressing.
30172244	3	33	theme	dynamic	534:540	arg1	base					549:552	the dynamic Schiff base	530:552	the dynamic Schiff base	530:552	By combining the dynamic Schiff base and copolymer micelle cross-linking in one system, a series of hydrogels were prepared by mixing quaternized chitosan (QCS) and benzaldehyde-terminated Pluronic®F127 (PF127-CHO) under physiological conditions.
30172244	9	34	theme	joints	1703:1708	arg1	healing					1721:1727	joints skin wound healing	1703:1727	joints skin wound healing	1703:1727	Taken together, the antibacterial adhesive hydrogels with self-healing and good mechanical property offer significant promise as dressing materials for joints skin wound healing.
30172244	1	35	theme	skin	355:358	arg1	healing					366:372	joints skin wound healing	348:372	joints skin wound healing	348:372	Designing wound dressing materials with outstanding therapeutic effects, self-healing, adhesiveness and suitable mechanical property has great practical significance in healthcare, especially for joints skin wound healing.
30172244	1	36	contain	has	285:287	arg2	significance					305:316	great practical significance	289:316	great practical significance	289:316	Designing wound dressing materials with outstanding therapeutic effects, self-healing, adhesiveness and suitable mechanical property has great practical significance in healthcare, especially for joints skin wound healing.
30172244	1	36	contain	has	285:287	arg1	self-healing					225:236	self-healing	225:236	self-healing	225:236	Designing wound dressing materials with outstanding therapeutic effects, self-healing, adhesiveness and suitable mechanical property has great practical significance in healthcare, especially for joints skin wound healing.
30172244	1	36	contain	has	285:287	arg1	property					276:283	suitable mechanical property	256:283	suitable mechanical property	256:283	Designing wound dressing materials with outstanding therapeutic effects, self-healing, adhesiveness and suitable mechanical property has great practical significance in healthcare, especially for joints skin wound healing.
30172244	1	36	contain	has	285:287	arg1	adhesiveness					239:250	adhesiveness	239:250	adhesiveness	239:250	Designing wound dressing materials with outstanding therapeutic effects, self-healing, adhesiveness and suitable mechanical property has great practical significance in healthcare, especially for joints skin wound healing.
30172244	5	37	with	stretchable	953:963	arg1	skin					1021:1024	human skin	1015:1024	human skin	1015:1024	The hydrogel dressings showed suitable stretchable and compressive property, comparable modulus with human skin, good adhesiveness and fast self-healing ability to bear deformation.
30172244	9	38	with	hydrogels	1594:1602	arg1	property					1642:1649	self-healing and good mechanical property	1609:1649	self-healing and good mechanical property	1609:1649	Taken together, the antibacterial adhesive hydrogels with self-healing and good mechanical property offer significant promise as dressing materials for joints skin wound healing.
30172244	2	39	with	kind	395:398	arg1	multi-functions					460:474	multi-functions	460:474	multi-functions as wound dressing for joint skin damage	460:514	Here, we designed a kind of self-healing injectable micelle/hydrogel composites with multi-functions as wound dressing for joint skin damage.
30172244	9	40	theme	wound	1715:1719	arg1	healing					1721:1727	joints skin wound healing	1703:1727	joints skin wound healing	1703:1727	Taken together, the antibacterial adhesive hydrogels with self-healing and good mechanical property offer significant promise as dressing materials for joints skin wound healing.
30172244	3	41	theme	copolymer	558:566	arg1	cross-linking					576:588	copolymer micelle cross-linking	558:588	copolymer micelle cross-linking	558:588	By combining the dynamic Schiff base and copolymer micelle cross-linking in one system, a series of hydrogels were prepared by mixing quaternized chitosan (QCS) and benzaldehyde-terminated Pluronic®F127 (PF127-CHO) under physiological conditions.
30172244	1	42	theme	mechanical	265:274	arg1	property					276:283	suitable mechanical property	256:283	suitable mechanical property	256:283	Designing wound dressing materials with outstanding therapeutic effects, self-healing, adhesiveness and suitable mechanical property has great practical significance in healthcare, especially for joints skin wound healing.
30172244	6	43	theme	efficient	1120:1128	arg1	performance					1141:1151	efficient hemostatic performance	1120:1151	efficient hemostatic performance	1120:1151	The hydrogels exhibited efficient hemostatic performance and biocompatibility.
30172244	7	44	dep	loaded	1198:1203	arg1	curcumin					1189:1196	curcumin	1189:1196	curcumin	1189:1196	Moreover, the curcumin loaded hydrogel showed good antioxidant ability and pH responsive release profiles.
30172244	7	45	theme	responsive	1253:1262	arg1	profiles					1272:1279	responsive release profiles	1253:1279	responsive release profiles	1253:1279	Moreover, the curcumin loaded hydrogel showed good antioxidant ability and pH responsive release profiles.
30172244	9	46	theme	adhesive	1585:1592	arg1	hydrogels					1594:1602	the antibacterial adhesive hydrogels	1567:1602	the antibacterial adhesive hydrogels with self-healing and good mechanical property	1567:1649	Taken together, the antibacterial adhesive hydrogels with self-healing and good mechanical property offer significant promise as dressing materials for joints skin wound healing.
30172244	8	47	theme	In	1282:1283	arg1	experiments					1290:1300	In vivo experiments	1282:1300	In vivo experiments	1282:1300	In vivo experiments indicated that curcumin loaded hydrogels significantly accelerated wound healing rate with higher granulation tissue thickness and collagen disposition and upregulated vascular endothelial growth factor (VEGF) in a full-thickness skin defect model.
30172244	9	48	theme	dressing	1680:1687	arg1	promise					1669:1675	significant promise	1657:1675	significant promise	1657:1675	Taken together, the antibacterial adhesive hydrogels with self-healing and good mechanical property offer significant promise as dressing materials for joints skin wound healing.
30172244	9	48	theme	dressing	1680:1687	arg1	materials					1689:1697	dressing materials	1680:1697	dressing materials for joints skin wound healing	1680:1727	Taken together, the antibacterial adhesive hydrogels with self-healing and good mechanical property offer significant promise as dressing materials for joints skin wound healing.
30172244	3	49	theme	physiological	738:750	arg1	conditions					752:761	physiological conditions	738:761	physiological conditions	738:761	By combining the dynamic Schiff base and copolymer micelle cross-linking in one system, a series of hydrogels were prepared by mixing quaternized chitosan (QCS) and benzaldehyde-terminated Pluronic®F127 (PF127-CHO) under physiological conditions.
30172244	1	50	theme	practical	295:303	arg1	significance					305:316	great practical significance	289:316	great practical significance	289:316	Designing wound dressing materials with outstanding therapeutic effects, self-healing, adhesiveness and suitable mechanical property has great practical significance in healthcare, especially for joints skin wound healing.
30172244	7	51	theme	antioxidant	1226:1236	arg1	ability					1238:1244	good antioxidant ability	1221:1244	good antioxidant ability	1221:1244	Moreover, the curcumin loaded hydrogel showed good antioxidant ability and pH responsive release profiles.
30172244	5	52	theme	compressive	969:979	arg1	property					981:988	compressive property	969:988	compressive property	969:988	The hydrogel dressings showed suitable stretchable and compressive property, comparable modulus with human skin, good adhesiveness and fast self-healing ability to bear deformation.
30172244	8	53	theme	higher	1393:1398	arg1	thickness					1419:1427	higher granulation tissue thickness	1393:1427	higher granulation tissue thickness	1393:1427	In vivo experiments indicated that curcumin loaded hydrogels significantly accelerated wound healing rate with higher granulation tissue thickness and collagen disposition and upregulated vascular endothelial growth factor (VEGF) in a full-thickness skin defect model.
30172244	1	54	theme	therapeutic	204:214	arg1	effects					216:222	therapeutic effects	204:222	therapeutic effects	204:222	Designing wound dressing materials with outstanding therapeutic effects, self-healing, adhesiveness and suitable mechanical property has great practical significance in healthcare, especially for joints skin wound healing.
30172244	8	55	theme	loaded	1326:1331	arg1	hydrogels					1333:1341	curcumin loaded hydrogels	1317:1341	curcumin loaded hydrogels	1317:1341	In vivo experiments indicated that curcumin loaded hydrogels significantly accelerated wound healing rate with higher granulation tissue thickness and collagen disposition and upregulated vascular endothelial growth factor (VEGF) in a full-thickness skin defect model.
30172244	8	56	theme	tissue	1412:1417	arg1	thickness					1419:1427	higher granulation tissue thickness	1393:1427	higher granulation tissue thickness	1393:1427	In vivo experiments indicated that curcumin loaded hydrogels significantly accelerated wound healing rate with higher granulation tissue thickness and collagen disposition and upregulated vascular endothelial growth factor (VEGF) in a full-thickness skin defect model.
30172244	5	57	with	adhesiveness	1032:1043	arg1	skin					1021:1024	human skin	1015:1024	human skin	1015:1024	The hydrogel dressings showed suitable stretchable and compressive property, comparable modulus with human skin, good adhesiveness and fast self-healing ability to bear deformation.
30172244	7	58	theme	loaded	1198:1203	arg1	hydrogel					1205:1212	the curcumin loaded hydrogel	1185:1212	the curcumin loaded hydrogel	1185:1212	Moreover, the curcumin loaded hydrogel showed good antioxidant ability and pH responsive release profiles.
30172244	5	59	theme	hydrogel	918:925	arg1	dressings					927:935	The hydrogel dressings	914:935	The hydrogel dressings	914:935	The hydrogel dressings showed suitable stretchable and compressive property, comparable modulus with human skin, good adhesiveness and fast self-healing ability to bear deformation.
30172244	8	60	theme	wound	1369:1373	arg1	healing					1375:1381	wound healing	1369:1381	wound healing rate	1369:1386	In vivo experiments indicated that curcumin loaded hydrogels significantly accelerated wound healing rate with higher granulation tissue thickness and collagen disposition and upregulated vascular endothelial growth factor (VEGF) in a full-thickness skin defect model.
30172244	5	61	with	ability	1067:1073	arg1	skin					1021:1024	human skin	1015:1024	human skin	1015:1024	The hydrogel dressings showed suitable stretchable and compressive property, comparable modulus with human skin, good adhesiveness and fast self-healing ability to bear deformation.
30172244	3	62	theme	hydrogels	617:625	arg1	series					607:612	a series	605:612	a series of hydrogels	605:625	By combining the dynamic Schiff base and copolymer micelle cross-linking in one system, a series of hydrogels were prepared by mixing quaternized chitosan (QCS) and benzaldehyde-terminated Pluronic®F127 (PF127-CHO) under physiological conditions.
30172244	5	63	theme	human	1015:1019	arg1	skin					1021:1024	human skin	1015:1024	human skin	1015:1024	The hydrogel dressings showed suitable stretchable and compressive property, comparable modulus with human skin, good adhesiveness and fast self-healing ability to bear deformation.
30172244	0	64	theme	wound	137:141	arg1	healing					143:149	skin wound healing	132:149	skin wound healing	132:149	Antibacterial adhesive injectable hydrogels with rapid self-healing, extensibility and compressibility as wound dressing for joints skin wound healing.
30172244	8	65	theme	endothelial	1479:1489	arg1	VEGF					1506:1509	VEGF	1506:1509	VEGF	1506:1509	In vivo experiments indicated that curcumin loaded hydrogels significantly accelerated wound healing rate with higher granulation tissue thickness and collagen disposition and upregulated vascular endothelial growth factor (VEGF) in a full-thickness skin defect model.
30172244	8	65	theme	endothelial	1479:1489	arg1	factor					1498:1503	vascular endothelial growth factor	1470:1503	vascular endothelial growth factor (VEGF)	1470:1510	In vivo experiments indicated that curcumin loaded hydrogels significantly accelerated wound healing rate with higher granulation tissue thickness and collagen disposition and upregulated vascular endothelial growth factor (VEGF) in a full-thickness skin defect model.
30172244	9	66	theme	skin	1710:1713	arg1	healing					1721:1727	joints skin wound healing	1703:1727	joints skin wound healing	1703:1727	Taken together, the antibacterial adhesive hydrogels with self-healing and good mechanical property offer significant promise as dressing materials for joints skin wound healing.
30172244	6	67	theme	hemostatic	1130:1139	arg1	performance					1141:1151	efficient hemostatic performance	1120:1151	efficient hemostatic performance	1120:1151	The hydrogels exhibited efficient hemostatic performance and biocompatibility.
30172244	1	68	theme	dressing	168:175	arg1	materials					177:185	wound dressing materials	162:185	wound dressing materials	162:185	Designing wound dressing materials with outstanding therapeutic effects, self-healing, adhesiveness and suitable mechanical property has great practical significance in healthcare, especially for joints skin wound healing.
30172244	4	69	theme	release	833:839	arg1	behavior					841:848	release behavior	833:848	release behavior	833:848	The inherent antibacterial property, pH-dependent biodegradation and release behavior were investigated to confirm multi-functions of wound dressing.
30172244	8	70	dep	In	1282:1283	arg1	vivo					1285:1288	vivo	1285:1288	vivo	1285:1288	In vivo experiments indicated that curcumin loaded hydrogels significantly accelerated wound healing rate with higher granulation tissue thickness and collagen disposition and upregulated vascular endothelial growth factor (VEGF) in a full-thickness skin defect model.
30172244	8	71	theme	full-thickness	1517:1530	arg1	model					1544:1548	a full-thickness skin defect model	1515:1548	a full-thickness skin defect model	1515:1548	In vivo experiments indicated that curcumin loaded hydrogels significantly accelerated wound healing rate with higher granulation tissue thickness and collagen disposition and upregulated vascular endothelial growth factor (VEGF) in a full-thickness skin defect model.
30172244	2	72	theme	injectable	416:425	arg1	composites					444:453	self-healing injectable micelle/hydrogel composites	403:453	self-healing injectable micelle/hydrogel composites	403:453	Here, we designed a kind of self-healing injectable micelle/hydrogel composites with multi-functions as wound dressing for joint skin damage.
30172244	0	73	theme	adhesive	14:21	arg1	hydrogels					34:42	Antibacterial adhesive injectable hydrogels	0:42	Antibacterial adhesive injectable hydrogels with rapid self-healing, extensibility and compressibility as wound dressing for joints	0:130	Antibacterial adhesive injectable hydrogels with rapid self-healing, extensibility and compressibility as wound dressing for joints skin wound healing.
30172244	8	74	theme	defect	1537:1542	arg1	model					1544:1548	a full-thickness skin defect model	1515:1548	a full-thickness skin defect model	1515:1548	In vivo experiments indicated that curcumin loaded hydrogels significantly accelerated wound healing rate with higher granulation tissue thickness and collagen disposition and upregulated vascular endothelial growth factor (VEGF) in a full-thickness skin defect model.
30172244	5	75	theme	fast	1049:1052	arg1	ability					1067:1073	fast self-healing ability	1049:1073	fast self-healing ability to bear deformation	1049:1093	The hydrogel dressings showed suitable stretchable and compressive property, comparable modulus with human skin, good adhesiveness and fast self-healing ability to bear deformation.
30172244	2	76	theme	wound	479:483	arg1	dressing					485:492	wound dressing	479:492	wound dressing for joint skin damage	479:514	Here, we designed a kind of self-healing injectable micelle/hydrogel composites with multi-functions as wound dressing for joint skin damage.
30172244	0	77	theme	rapid	49:53	arg1	self-healing					55:66	rapid self-healing	49:66	rapid self-healing	49:66	Antibacterial adhesive injectable hydrogels with rapid self-healing, extensibility and compressibility as wound dressing for joints skin wound healing.
30172244	4	78	theme	wound	898:902	arg1	dressing					904:911	wound dressing	898:911	wound dressing	898:911	The inherent antibacterial property, pH-dependent biodegradation and release behavior were investigated to confirm multi-functions of wound dressing.
30172244	0	79	with	hydrogels	34:42	arg1	compressibility					87:101	compressibility	87:101	compressibility	87:101	Antibacterial adhesive injectable hydrogels with rapid self-healing, extensibility and compressibility as wound dressing for joints skin wound healing.
30172244	0	79	with	hydrogels	34:42	arg1	extensibility					69:81	extensibility	69:81	extensibility	69:81	Antibacterial adhesive injectable hydrogels with rapid self-healing, extensibility and compressibility as wound dressing for joints skin wound healing.
30172244	0	79	with	hydrogels	34:42	arg1	self-healing					55:66	rapid self-healing	49:66	rapid self-healing	49:66	Antibacterial adhesive injectable hydrogels with rapid self-healing, extensibility and compressibility as wound dressing for joints skin wound healing.
30172244	2	80	theme	composites	444:453	arg1	kind					395:398	a kind	393:398	a kind of self-healing injectable micelle/hydrogel composites with multi-functions as wound dressing for joint skin damage	393:514	Here, we designed a kind of self-healing injectable micelle/hydrogel composites with multi-functions as wound dressing for joint skin damage.
30172244	1	81	theme	joints	348:353	arg1	healing					366:372	joints skin wound healing	348:372	joints skin wound healing	348:372	Designing wound dressing materials with outstanding therapeutic effects, self-healing, adhesiveness and suitable mechanical property has great practical significance in healthcare, especially for joints skin wound healing.
30172244	2	82	theme	skin	504:507	arg1	damage					509:514	joint skin damage	498:514	joint skin damage	498:514	Here, we designed a kind of self-healing injectable micelle/hydrogel composites with multi-functions as wound dressing for joint skin damage.
30172244	5	83	with	property	981:988	arg1	skin					1021:1024	human skin	1015:1024	human skin	1015:1024	The hydrogel dressings showed suitable stretchable and compressive property, comparable modulus with human skin, good adhesiveness and fast self-healing ability to bear deformation.
30172244	1	84	theme	wound	162:166	arg1	materials					177:185	wound dressing materials	162:185	wound dressing materials	162:185	Designing wound dressing materials with outstanding therapeutic effects, self-healing, adhesiveness and suitable mechanical property has great practical significance in healthcare, especially for joints skin wound healing.
30172244	3	85	theme	Schiff	542:547	arg1	base					549:552	the dynamic Schiff base	530:552	the dynamic Schiff base	530:552	By combining the dynamic Schiff base and copolymer micelle cross-linking in one system, a series of hydrogels were prepared by mixing quaternized chitosan (QCS) and benzaldehyde-terminated Pluronic®F127 (PF127-CHO) under physiological conditions.
30172244	1	86	theme	wound	360:364	arg1	healing					366:372	joints skin wound healing	348:372	joints skin wound healing	348:372	Designing wound dressing materials with outstanding therapeutic effects, self-healing, adhesiveness and suitable mechanical property has great practical significance in healthcare, especially for joints skin wound healing.
30172244	8	87	dep	loaded	1326:1331	arg1	curcumin					1317:1324	curcumin	1317:1324	curcumin	1317:1324	In vivo experiments indicated that curcumin loaded hydrogels significantly accelerated wound healing rate with higher granulation tissue thickness and collagen disposition and upregulated vascular endothelial growth factor (VEGF) in a full-thickness skin defect model.
30172244	1	88	theme	suitable	256:263	arg1	property					276:283	suitable mechanical property	256:283	suitable mechanical property	256:283	Designing wound dressing materials with outstanding therapeutic effects, self-healing, adhesiveness and suitable mechanical property has great practical significance in healthcare, especially for joints skin wound healing.
30172244	3	89	theme	micelle	568:574	arg1	cross-linking					576:588	copolymer micelle cross-linking	558:588	copolymer micelle cross-linking	558:588	By combining the dynamic Schiff base and copolymer micelle cross-linking in one system, a series of hydrogels were prepared by mixing quaternized chitosan (QCS) and benzaldehyde-terminated Pluronic®F127 (PF127-CHO) under physiological conditions.
30172244	9	90	theme	antibacterial	1571:1583	arg1	hydrogels					1594:1602	the antibacterial adhesive hydrogels	1567:1602	the antibacterial adhesive hydrogels with self-healing and good mechanical property	1567:1649	Taken together, the antibacterial adhesive hydrogels with self-healing and good mechanical property offer significant promise as dressing materials for joints skin wound healing.
31718523	5	0	theme	RNA	593:595	arg1	genes					597:601	ribosomal RNA genes	583:601	ribosomal RNA genes	583:601	The fecal gut microbiota was analyzed by sequencing 16S ribosomal RNA genes.
31718523	2	1	theme	high-fat	318:325	arg1	HFD					333:335	HFD	333:335	HFD	333:335	Here, we studied the effects of CN supplementation and voluntary exercise on obesity and gut microbiota in high-fat diet (HFD)-fed mice.
31718523	2	1	theme	high-fat	318:325	arg1	diet					327:330	high-fat diet	318:330	high-fat diet (HFD)-fed mice	318:345	Here, we studied the effects of CN supplementation and voluntary exercise on obesity and gut microbiota in high-fat diet (HFD)-fed mice.
31718523	0	2	theme	gut	66:68	arg1	microbiota					70:79	gut microbiota	66:79	gut microbiota	66:79	Effect of dietary cellulose nanofiber and exercise on obesity and gut microbiota in mice fed a high-fat-diet.
31718523	4	3	theme	body	469:472	arg1	weight					474:479	body weight	469:479	body weight	469:479	CN intake and exercise together suppressed the increase in body weight and fat mass, and improved glucose tolerance.
31718523	1	4	with	consumption	135:145	arg1	exercise					152:159	exercise	152:159	exercise	152:159	Cellulose nanofiber (CN) consumption with exercise could be a potential strategy to control obesity.
31718523	5	5	theme	gut	537:539	arg1	microbiota					541:550	The fecal gut microbiota	527:550	The fecal gut microbiota	527:550	The fecal gut microbiota was analyzed by sequencing 16S ribosomal RNA genes.
31718523	4	6	theme	CN	410:411	arg1	intake					413:418	CN intake	410:418	CN intake	410:418	CN intake and exercise together suppressed the increase in body weight and fat mass, and improved glucose tolerance.
31718523	9	7	theme	butyrate	931:938	arg1	families					918:925	These two families	908:925	These two families	908:925	These two families are butyrate producers.
31718523	9	7	theme	butyrate	931:938	arg1	producers					940:948	butyrate producers	931:948	butyrate producers	931:948	These two families are butyrate producers.
31718523	0	8	from	Effect	0:5	arg1	obesity					54:60	obesity	54:60	obesity	54:60	Effect of dietary cellulose nanofiber and exercise on obesity and gut microbiota in mice fed a high-fat-diet.
31718523	0	8	from	Effect	0:5	arg1	microbiota					70:79	gut microbiota	66:79	gut microbiota	66:79	Effect of dietary cellulose nanofiber and exercise on obesity and gut microbiota in mice fed a high-fat-diet.
31718523	4	9	from	increase	457:464	arg1	weight					474:479	body weight	469:479	body weight	469:479	CN intake and exercise together suppressed the increase in body weight and fat mass, and improved glucose tolerance.
31718523	4	9	from	increase	457:464	arg1	mass					489:492	fat mass	485:492	fat mass	485:492	CN intake and exercise together suppressed the increase in body weight and fat mass, and improved glucose tolerance.
31718523	2	10	from	obesity	288:294	arg1	mice					342:345	high-fat diet (HFD)-fed mice	318:345	high-fat diet (HFD)-fed mice	318:345	Here, we studied the effects of CN supplementation and voluntary exercise on obesity and gut microbiota in high-fat diet (HFD)-fed mice.
31718523	2	11	theme	supplementation	246:260	arg1	effects					232:238	the effects	228:238	the effects of CN supplementation and voluntary exercise on obesity and gut microbiota in high-fat diet (HFD)-fed mice	228:345	Here, we studied the effects of CN supplementation and voluntary exercise on obesity and gut microbiota in high-fat diet (HFD)-fed mice.
31718523	6	12	from	shift	644:648	arg1	composition					668:678	the microbiota composition	653:678	the microbiota composition resulting from exercise, but not from CN supplementation	653:735	Principal component analysis revealed a shift in the microbiota composition resulting from exercise, but not from CN supplementation.
31718523	2	13	from	microbiota	304:313	arg1	mice					342:345	high-fat diet (HFD)-fed mice	318:345	high-fat diet (HFD)-fed mice	318:345	Here, we studied the effects of CN supplementation and voluntary exercise on obesity and gut microbiota in high-fat diet (HFD)-fed mice.
31718523	2	14	theme	CN	243:244	arg1	supplementation					246:260	CN supplementation	243:260	CN supplementation	243:260	Here, we studied the effects of CN supplementation and voluntary exercise on obesity and gut microbiota in high-fat diet (HFD)-fed mice.
31718523	6	15	theme	microbiota	657:666	arg1	composition					668:678	the microbiota composition	653:678	the microbiota composition resulting from exercise, but not from CN supplementation	653:735	Principal component analysis revealed a shift in the microbiota composition resulting from exercise, but not from CN supplementation.
31718523	10	16	from	amount	974:979	arg1	cecum					999:1003	the cecum	995:1003	the cecum	995:1003	Exercise increased the amount of acetate in the cecum.
31718523	11	17	theme	microbiota	1147:1156	arg1	balance					1128:1134	the balance	1124:1134	the balance of the gut microbiota	1124:1156	These results suggest that CN consumption improves exercise performance and exerts anti-obesity effects by modulating the balance of the gut microbiota.
31718523	3	18	theme	wheel	386:390	arg1	running					392:398	voluntary wheel running	376:398	voluntary wheel running activity	376:407	Consumption of CN increased voluntary wheel running activity.
31718523	2	19	theme	-fed	337:340	arg1	mice					342:345	high-fat diet (HFD)-fed mice	318:345	high-fat diet (HFD)-fed mice	318:345	Here, we studied the effects of CN supplementation and voluntary exercise on obesity and gut microbiota in high-fat diet (HFD)-fed mice.
31718523	8	20	theme	CN	863:864	arg1	intake					866:871	CN intake	863:871	CN intake	863:871	Exercise also increased Ruminococcaceae, whereas exercise and CN intake together increased Eubacteriaceae.
31718523	11	21	theme	exercise	1057:1064	arg1	performance					1066:1076	exercise performance	1057:1076	exercise performance	1057:1076	These results suggest that CN consumption improves exercise performance and exerts anti-obesity effects by modulating the balance of the gut microbiota.
31718523	5	22	theme	ribosomal	583:591	arg1	genes					597:601	ribosomal RNA genes	583:601	ribosomal RNA genes	583:601	The fecal gut microbiota was analyzed by sequencing 16S ribosomal RNA genes.
31718523	2	23	theme	exercise	276:283	arg1	effects					232:238	the effects	228:238	the effects of CN supplementation and voluntary exercise on obesity and gut microbiota in high-fat diet (HFD)-fed mice	228:345	Here, we studied the effects of CN supplementation and voluntary exercise on obesity and gut microbiota in high-fat diet (HFD)-fed mice.
31718523	3	24	theme	voluntary	376:384	arg1	running					392:398	voluntary wheel running	376:398	voluntary wheel running activity	376:407	Consumption of CN increased voluntary wheel running activity.
31718523	0	25	theme	cellulose	18:26	arg1	Effect					0:5	Effect	0:5	Effect of dietary cellulose nanofiber and exercise on obesity and gut microbiota in mice	0:87	Effect of dietary cellulose nanofiber and exercise on obesity and gut microbiota in mice fed a high-fat-diet.
31718523	5	26	theme	fecal	531:535	arg1	microbiota					541:550	The fecal gut microbiota	527:550	The fecal gut microbiota	527:550	The fecal gut microbiota was analyzed by sequencing 16S ribosomal RNA genes.
31718523	2	27	theme	voluntary	266:274	arg1	exercise					276:283	voluntary exercise	266:283	voluntary exercise	266:283	Here, we studied the effects of CN supplementation and voluntary exercise on obesity and gut microbiota in high-fat diet (HFD)-fed mice.
31718523	0	28	theme	dietary	10:16	arg1	cellulose					18:26	dietary cellulose nanofiber and exercise	10:49	dietary cellulose nanofiber and exercise	10:49	Effect of dietary cellulose nanofiber and exercise on obesity and gut microbiota in mice fed a high-fat-diet.
31718523	4	29	theme	glucose	508:514	arg1	tolerance					516:524	glucose tolerance	508:524	glucose tolerance	508:524	CN intake and exercise together suppressed the increase in body weight and fat mass, and improved glucose tolerance.
31718523	0	30	from	microbiota	70:79	arg1	mice					84:87	mice	84:87	mice	84:87	Effect of dietary cellulose nanofiber and exercise on obesity and gut microbiota in mice fed a high-fat-diet.
31718523	11	31	theme	CN	1033:1034	arg1	consumption					1036:1046	CN consumption	1033:1046	CN consumption	1033:1046	These results suggest that CN consumption improves exercise performance and exerts anti-obesity effects by modulating the balance of the gut microbiota.
31718523	1	32	theme	potential	172:180	arg1	strategy					182:189	a potential strategy	170:189	a potential strategy to control obesity	170:208	Cellulose nanofiber (CN) consumption with exercise could be a potential strategy to control obesity.
31718523	1	32	theme	potential	172:180	arg1	consumption					135:145	Cellulose nanofiber (CN) consumption	110:145	Cellulose nanofiber (CN) consumption with exercise	110:159	Cellulose nanofiber (CN) consumption with exercise could be a potential strategy to control obesity.
31718523	6	33	theme	component	614:622	arg1	analysis					624:631	Principal component analysis	604:631	Principal component analysis	604:631	Principal component analysis revealed a shift in the microbiota composition resulting from exercise, but not from CN supplementation.
31718523	11	34	theme	anti-obesity	1089:1100	arg1	effects					1102:1108	anti-obesity effects	1089:1108	anti-obesity effects	1089:1108	These results suggest that CN consumption improves exercise performance and exerts anti-obesity effects by modulating the balance of the gut microbiota.
31718523	3	35	theme	CN	363:364	arg1	Consumption					348:358	Consumption	348:358	Consumption of CN	348:364	Consumption of CN increased voluntary wheel running activity.
31718523	0	36	from	obesity	54:60	arg1	mice					84:87	mice	84:87	mice	84:87	Effect of dietary cellulose nanofiber and exercise on obesity and gut microbiota in mice fed a high-fat-diet.
31718523	6	37	theme	CN	718:719	arg1	supplementation					721:735	CN supplementation	718:735	CN supplementation	718:735	Principal component analysis revealed a shift in the microbiota composition resulting from exercise, but not from CN supplementation.
31718523	2	38	theme	gut	300:302	arg1	microbiota					304:313	gut microbiota	300:313	gut microbiota	300:313	Here, we studied the effects of CN supplementation and voluntary exercise on obesity and gut microbiota in high-fat diet (HFD)-fed mice.
31718523	6	39	theme	Principal	604:612	arg1	analysis					624:631	Principal component analysis	604:631	Principal component analysis	604:631	Principal component analysis revealed a shift in the microbiota composition resulting from exercise, but not from CN supplementation.
31718523	4	40	theme	fat	485:487	arg1	mass					489:492	fat mass	485:492	fat mass	485:492	CN intake and exercise together suppressed the increase in body weight and fat mass, and improved glucose tolerance.
31718523	0	41	dep	cellulose	18:26	arg1	exercise					42:49	exercise	42:49	exercise	42:49	Effect of dietary cellulose nanofiber and exercise on obesity and gut microbiota in mice fed a high-fat-diet.
31718523	0	41	dep	cellulose	18:26	arg1	nanofiber					28:36	nanofiber	28:36	nanofiber	28:36	Effect of dietary cellulose nanofiber and exercise on obesity and gut microbiota in mice fed a high-fat-diet.
31718523	1	42	theme	Cellulose	110:118	arg1	CN					131:132	CN	131:132	CN	131:132	Cellulose nanofiber (CN) consumption with exercise could be a potential strategy to control obesity.
31718523	1	42	theme	Cellulose	110:118	arg1	nanofiber					120:128	Cellulose nanofiber	110:128	Cellulose nanofiber (CN) consumption with exercise	110:159	Cellulose nanofiber (CN) consumption with exercise could be a potential strategy to control obesity.
31718523	2	43	from	effects	232:238	arg1	obesity					288:294	obesity	288:294	obesity	288:294	Here, we studied the effects of CN supplementation and voluntary exercise on obesity and gut microbiota in high-fat diet (HFD)-fed mice.
31718523	2	43	from	effects	232:238	arg1	microbiota					304:313	gut microbiota	300:313	gut microbiota	300:313	Here, we studied the effects of CN supplementation and voluntary exercise on obesity and gut microbiota in high-fat diet (HFD)-fed mice.
31718523	1	44	theme	nanofiber	120:128	arg1	strategy					182:189	a potential strategy	170:189	a potential strategy to control obesity	170:208	Cellulose nanofiber (CN) consumption with exercise could be a potential strategy to control obesity.
31718523	1	44	theme	nanofiber	120:128	arg1	consumption					135:145	Cellulose nanofiber (CN) consumption	110:145	Cellulose nanofiber (CN) consumption with exercise	110:159	Cellulose nanofiber (CN) consumption with exercise could be a potential strategy to control obesity.
31718523	11	45	theme	gut	1143:1145	arg1	microbiota					1147:1156	the gut microbiota	1139:1156	the gut microbiota	1139:1156	These results suggest that CN consumption improves exercise performance and exerts anti-obesity effects by modulating the balance of the gut microbiota.
31718523	3	46	theme	running	392:398	arg1	activity					400:407	voluntary wheel running activity	376:407	voluntary wheel running activity	376:407	Consumption of CN increased voluntary wheel running activity.
31718523	10	47	theme	acetate	984:990	arg1	amount					974:979	the amount	970:979	the amount of acetate in the cecum	970:1003	Exercise increased the amount of acetate in the cecum.
31718523	10	47	theme	acetate	984:990	arg1	acetate					984:990	acetate	984:990	acetate	984:990	Exercise increased the amount of acetate in the cecum.
31718523	2	48	theme	diet	327:330	arg1	mice					342:345	high-fat diet (HFD)-fed mice	318:345	high-fat diet (HFD)-fed mice	318:345	Here, we studied the effects of CN supplementation and voluntary exercise on obesity and gut microbiota in high-fat diet (HFD)-fed mice.
31003566	2	0	theme	potentially-prebiotic	339:359	arg1	fibers					369:374	potentially-prebiotic dietary fibers	339:374	potentially-prebiotic dietary fibers	339:374	We demonstrated the applicability of a mictotiter plate in the in vitro fermentation models for the screening of potentially-prebiotic dietary fibers.
31003566	8	1	theme	oat	1452:1454	arg1	fractions					1430:1438	Insoluble and soluble rye bran fractions	1399:1438	Insoluble and soluble rye bran fractions	1399:1438	Insoluble and soluble rye bran fractions and soluble oat fiber were the best for controlling the level of proteobacteria at the level below 2%.
31003566	8	1	theme	oat	1452:1454	arg1	fiber					1456:1460	soluble oat fiber	1444:1460	soluble oat fiber	1444:1460	Insoluble and soluble rye bran fractions and soluble oat fiber were the best for controlling the level of proteobacteria at the level below 2%.
31003566	8	1	theme	oat	1452:1454	arg1	best					1471:1474	best	1471:1474	best	1471:1474	Insoluble and soluble rye bran fractions and soluble oat fiber were the best for controlling the level of proteobacteria at the level below 2%.
31003566	3	2	theme	fatty	511:515	arg1	acid					517:520	short-chain fatty acid	499:520	short-chain fatty acid production	499:531	The effects of seven rye bran-, oat- and linseed-derived fiber preparations on the human fecal microbiota composition and short-chain fatty acid production were studied.
31003566	6	3	theme	significant	1148:1158	arg1	increase					1160:1167	a significant increase	1146:1167	a significant increase in butyrate production	1146:1190	The fermentation of rye bran, enzymatically treated rye bran, its insoluble fraction, soluble oat fiber and a mixture of rye fiber:soluble oat fiber:linseed resulted in a significant increase in butyrate production and a bifidogenic effect in the absence of the antibiotic.
31003566	2	4	theme	mictotiter	265:274	arg1	plate					276:280	a mictotiter plate	263:280	a mictotiter plate	263:280	We demonstrated the applicability of a mictotiter plate in the in vitro fermentation models for the screening of potentially-prebiotic dietary fibers.
31003566	2	5	theme	fermentation	298:309	arg1	models					311:316	the in vitro fermentation models	285:316	the in vitro fermentation models for the screening of potentially-prebiotic dietary fibers	285:374	We demonstrated the applicability of a mictotiter plate in the in vitro fermentation models for the screening of potentially-prebiotic dietary fibers.
31003566	5	6	theme	Sutterilaceae	916:928	arg1	levels					930:935	proteobacterial Sutterilaceae levels	900:935	proteobacterial Sutterilaceae levels	900:935	The antibiotic induced a shift in the bacterial community in the absence of fibers by decreasing the relative amounts of Bifidobacteriaceae, Bacteroidaceae, Prevotellaceae, Lachnospiraceae and Ruminococcaceae, and increasing proteobacterial Sutterilaceae levels from 1% to 11% of the total microbiota.
31003566	7	7	theme	relative	1364:1371	arg1	bifidobacteria					1383:1396	bifidobacteria	1383:1396	bifidobacteria	1383:1396	These fibers were also able to counteract the negative effects of the antibiotic and prevent the decrease in the relative amount of bifidobacteria.
31003566	7	7	theme	relative	1364:1371	arg1	amount					1373:1378	the relative amount	1360:1378	the relative amount of bifidobacteria	1360:1396	These fibers were also able to counteract the negative effects of the antibiotic and prevent the decrease in the relative amount of bifidobacteria.
31003566	4	8	theme	amoxicillin-clavulanate	632:654	arg1	effects					621:627	the harmful effects	609:627	the harmful effects of amoxicillin-clavulanate on the microbiota	609:672	The model was also used to study whether fibers can alleviate the harmful effects of amoxicillin-clavulanate on the microbiota.
31003566	5	9	theme	bacterial	713:721	arg1	community					723:731	the bacterial community	709:731	the bacterial community in the absence of fibers	709:756	The antibiotic induced a shift in the bacterial community in the absence of fibers by decreasing the relative amounts of Bifidobacteriaceae, Bacteroidaceae, Prevotellaceae, Lachnospiraceae and Ruminococcaceae, and increasing proteobacterial Sutterilaceae levels from 1% to 11% of the total microbiota.
31003566	2	10	theme	in	289:290	arg1	models					311:316	the in vitro fermentation models	285:316	the in vitro fermentation models for the screening of potentially-prebiotic dietary fibers	285:374	We demonstrated the applicability of a mictotiter plate in the in vitro fermentation models for the screening of potentially-prebiotic dietary fibers.
31003566	8	11	theme	rye	1421:1423	arg1	fractions					1430:1438	Insoluble and soluble rye bran fractions	1399:1438	Insoluble and soluble rye bran fractions	1399:1438	Insoluble and soluble rye bran fractions and soluble oat fiber were the best for controlling the level of proteobacteria at the level below 2%.
31003566	8	11	theme	rye	1421:1423	arg1	fiber					1456:1460	soluble oat fiber	1444:1460	soluble oat fiber	1444:1460	Insoluble and soluble rye bran fractions and soluble oat fiber were the best for controlling the level of proteobacteria at the level below 2%.
31003566	8	11	theme	rye	1421:1423	arg1	best					1471:1474	best	1471:1474	best	1471:1474	Insoluble and soluble rye bran fractions and soluble oat fiber were the best for controlling the level of proteobacteria at the level below 2%.
31003566	6	12	theme	oat	1071:1073	arg1	fiber					1075:1079	soluble oat fiber	1063:1079	soluble oat fiber	1063:1079	The fermentation of rye bran, enzymatically treated rye bran, its insoluble fraction, soluble oat fiber and a mixture of rye fiber:soluble oat fiber:linseed resulted in a significant increase in butyrate production and a bifidogenic effect in the absence of the antibiotic.
31003566	1	13	theme	gut	210:212	arg1	microbiota					214:223	the gut microbiota	206:223	the gut microbiota	206:223	The development of prebiotic fibers requires fast high-throughput screening of their effects on the gut microbiota.
31003566	1	14	from	effects	195:201	arg1	microbiota					214:223	the gut microbiota	206:223	the gut microbiota	206:223	The development of prebiotic fibers requires fast high-throughput screening of their effects on the gut microbiota.
31003566	8	15	theme	soluble	1444:1450	arg1	fractions					1430:1438	Insoluble and soluble rye bran fractions	1399:1438	Insoluble and soluble rye bran fractions	1399:1438	Insoluble and soluble rye bran fractions and soluble oat fiber were the best for controlling the level of proteobacteria at the level below 2%.
31003566	8	15	theme	soluble	1444:1450	arg1	fiber					1456:1460	soluble oat fiber	1444:1460	soluble oat fiber	1444:1460	Insoluble and soluble rye bran fractions and soluble oat fiber were the best for controlling the level of proteobacteria at the level below 2%.
31003566	8	15	theme	soluble	1444:1450	arg1	best					1471:1474	best	1471:1474	best	1471:1474	Insoluble and soluble rye bran fractions and soluble oat fiber were the best for controlling the level of proteobacteria at the level below 2%.
31003566	1	16	theme	fibers	139:144	arg1	development					114:124	The development	110:124	The development of prebiotic fibers	110:144	The development of prebiotic fibers requires fast high-throughput screening of their effects on the gut microbiota.
31003566	6	17	theme	rye	1029:1031	arg1	bran					1033:1036	enzymatically treated rye bran	1007:1036	enzymatically treated rye bran	1007:1036	The fermentation of rye bran, enzymatically treated rye bran, its insoluble fraction, soluble oat fiber and a mixture of rye fiber:soluble oat fiber:linseed resulted in a significant increase in butyrate production and a bifidogenic effect in the absence of the antibiotic.
31003566	0	18	theme	Microbiota	58:67	arg1	Effects					69:75	the Gut Microbiota Effects	50:75	the Gut Microbiota Effects of Different Fiber Preparations	50:107	A Small In Vitro Fermentation Model for Screening the Gut Microbiota Effects of Different Fiber Preparations.
31003566	1	19	theme	fast	155:158	arg1	screening					176:184	fast high-throughput screening	155:184	fast high-throughput screening of their effects on the gut microbiota	155:223	The development of prebiotic fibers requires fast high-throughput screening of their effects on the gut microbiota.
31003566	5	20	dep	%	950:950	arg1	to					945:946	to	945:946	to	945:946	The antibiotic induced a shift in the bacterial community in the absence of fibers by decreasing the relative amounts of Bifidobacteriaceae, Bacteroidaceae, Prevotellaceae, Lachnospiraceae and Ruminococcaceae, and increasing proteobacterial Sutterilaceae levels from 1% to 11% of the total microbiota.
31003566	6	21	theme	bran	1001:1004	arg1	fermentation					981:992	The fermentation	977:992	The fermentation of rye bran, enzymatically treated rye bran, its insoluble fraction, soluble oat fiber and a mixture of rye fiber:soluble oat fiber:linseed	977:1132	The fermentation of rye bran, enzymatically treated rye bran, its insoluble fraction, soluble oat fiber and a mixture of rye fiber:soluble oat fiber:linseed resulted in a significant increase in butyrate production and a bifidogenic effect in the absence of the antibiotic.
31003566	3	22	theme	fiber	434:438	arg1	preparations					440:451	seven rye bran-, oat- and linseed-derived fiber preparations	392:451	seven rye bran-, oat- and linseed-derived fiber preparations	392:451	The effects of seven rye bran-, oat- and linseed-derived fiber preparations on the human fecal microbiota composition and short-chain fatty acid production were studied.
31003566	5	23	theme	fibers	751:756	arg1	absence					740:746	the absence	736:746	the absence of fibers	736:756	The antibiotic induced a shift in the bacterial community in the absence of fibers by decreasing the relative amounts of Bifidobacteriaceae, Bacteroidaceae, Prevotellaceae, Lachnospiraceae and Ruminococcaceae, and increasing proteobacterial Sutterilaceae levels from 1% to 11% of the total microbiota.
31003566	0	24	theme	Gut	54:56	arg1	Effects					69:75	the Gut Microbiota Effects	50:75	the Gut Microbiota Effects of Different Fiber Preparations	50:107	A Small In Vitro Fermentation Model for Screening the Gut Microbiota Effects of Different Fiber Preparations.
31003566	6	25	from	increase	1160:1167	arg1	absence					1224:1230	the absence	1220:1230	the absence of the antibiotic	1220:1248	The fermentation of rye bran, enzymatically treated rye bran, its insoluble fraction, soluble oat fiber and a mixture of rye fiber:soluble oat fiber:linseed resulted in a significant increase in butyrate production and a bifidogenic effect in the absence of the antibiotic.
31003566	6	25	from	increase	1160:1167	arg1	production					1181:1190	butyrate production	1172:1190	butyrate production	1172:1190	The fermentation of rye bran, enzymatically treated rye bran, its insoluble fraction, soluble oat fiber and a mixture of rye fiber:soluble oat fiber:linseed resulted in a significant increase in butyrate production and a bifidogenic effect in the absence of the antibiotic.
31003566	5	26	theme	total	959:963	arg1	microbiota					965:974	the total microbiota	955:974	the total microbiota	955:974	The antibiotic induced a shift in the bacterial community in the absence of fibers by decreasing the relative amounts of Bifidobacteriaceae, Bacteroidaceae, Prevotellaceae, Lachnospiraceae and Ruminococcaceae, and increasing proteobacterial Sutterilaceae levels from 1% to 11% of the total microbiota.
31003566	2	27	from	applicability	246:258	arg1	models					311:316	the in vitro fermentation models	285:316	the in vitro fermentation models for the screening of potentially-prebiotic dietary fibers	285:374	We demonstrated the applicability of a mictotiter plate in the in vitro fermentation models for the screening of potentially-prebiotic dietary fibers.
31003566	7	28	theme	negative	1297:1304	arg1	effects					1306:1312	the negative effects	1293:1312	the negative effects of the antibiotic	1293:1330	These fibers were also able to counteract the negative effects of the antibiotic and prevent the decrease in the relative amount of bifidobacteria.
31003566	3	29	theme	human	460:464	arg1	composition					483:493	the human fecal microbiota composition	456:493	the human fecal microbiota composition	456:493	The effects of seven rye bran-, oat- and linseed-derived fiber preparations on the human fecal microbiota composition and short-chain fatty acid production were studied.
31003566	0	30	theme	In	8:9	arg1	Model					30:34	A Small In Vitro Fermentation Model	0:34	A Small In Vitro Fermentation Model for Screening the Gut Microbiota Effects of Different Fiber Preparations	0:107	A Small In Vitro Fermentation Model for Screening the Gut Microbiota Effects of Different Fiber Preparations.
31003566	0	31	theme	Fermentation	17:28	arg1	Model					30:34	A Small In Vitro Fermentation Model	0:34	A Small In Vitro Fermentation Model for Screening the Gut Microbiota Effects of Different Fiber Preparations	0:107	A Small In Vitro Fermentation Model for Screening the Gut Microbiota Effects of Different Fiber Preparations.
31003566	6	32	theme	insoluble	1043:1051	arg1	fraction					1053:1060	its insoluble fraction	1039:1060	its insoluble fraction	1039:1060	The fermentation of rye bran, enzymatically treated rye bran, its insoluble fraction, soluble oat fiber and a mixture of rye fiber:soluble oat fiber:linseed resulted in a significant increase in butyrate production and a bifidogenic effect in the absence of the antibiotic.
31003566	3	33	theme	rye	398:400	arg1	preparations					440:451	seven rye bran-, oat- and linseed-derived fiber preparations	392:451	seven rye bran-, oat- and linseed-derived fiber preparations	392:451	The effects of seven rye bran-, oat- and linseed-derived fiber preparations on the human fecal microbiota composition and short-chain fatty acid production were studied.
31003566	6	34	theme	oat	1116:1118	arg1	fiber					1120:1124	soluble oat fiber	1108:1124	The fermentation of rye bran, enzymatically treated rye bran, its insoluble fraction, soluble oat fiber and a mixture of rye fiber:soluble oat fiber:linseed	977:1132	The fermentation of rye bran, enzymatically treated rye bran, its insoluble fraction, soluble oat fiber and a mixture of rye fiber:soluble oat fiber:linseed resulted in a significant increase in butyrate production and a bifidogenic effect in the absence of the antibiotic.
31003566	6	35	theme	butyrate	1172:1179	arg1	production					1181:1190	butyrate production	1172:1190	butyrate production	1172:1190	The fermentation of rye bran, enzymatically treated rye bran, its insoluble fraction, soluble oat fiber and a mixture of rye fiber:soluble oat fiber:linseed resulted in a significant increase in butyrate production and a bifidogenic effect in the absence of the antibiotic.
31003566	8	36	theme	Insoluble	1399:1407	arg1	fractions					1430:1438	Insoluble and soluble rye bran fractions	1399:1438	Insoluble and soluble rye bran fractions	1399:1438	Insoluble and soluble rye bran fractions and soluble oat fiber were the best for controlling the level of proteobacteria at the level below 2%.
31003566	8	36	theme	Insoluble	1399:1407	arg1	fiber					1456:1460	soluble oat fiber	1444:1460	soluble oat fiber	1444:1460	Insoluble and soluble rye bran fractions and soluble oat fiber were the best for controlling the level of proteobacteria at the level below 2%.
31003566	8	36	theme	Insoluble	1399:1407	arg1	best					1471:1474	best	1471:1474	best	1471:1474	Insoluble and soluble rye bran fractions and soluble oat fiber were the best for controlling the level of proteobacteria at the level below 2%.
31003566	3	37	theme	microbiota	472:481	arg1	composition					483:493	the human fecal microbiota composition	456:493	the human fecal microbiota composition	456:493	The effects of seven rye bran-, oat- and linseed-derived fiber preparations on the human fecal microbiota composition and short-chain fatty acid production were studied.
31003566	5	38	from	shift	700:704	arg1	community					723:731	the bacterial community	709:731	the bacterial community in the absence of fibers	709:756	The antibiotic induced a shift in the bacterial community in the absence of fibers by decreasing the relative amounts of Bifidobacteriaceae, Bacteroidaceae, Prevotellaceae, Lachnospiraceae and Ruminococcaceae, and increasing proteobacterial Sutterilaceae levels from 1% to 11% of the total microbiota.
31003566	0	39	dep	In	8:9	arg1	Vitro					11:15	Vitro	11:15	Vitro	11:15	A Small In Vitro Fermentation Model for Screening the Gut Microbiota Effects of Different Fiber Preparations.
31003566	8	40	theme	soluble	1413:1419	arg1	fractions					1430:1438	Insoluble and soluble rye bran fractions	1399:1438	Insoluble and soluble rye bran fractions	1399:1438	Insoluble and soluble rye bran fractions and soluble oat fiber were the best for controlling the level of proteobacteria at the level below 2%.
31003566	8	40	theme	soluble	1413:1419	arg1	fiber					1456:1460	soluble oat fiber	1444:1460	soluble oat fiber	1444:1460	Insoluble and soluble rye bran fractions and soluble oat fiber were the best for controlling the level of proteobacteria at the level below 2%.
31003566	8	40	theme	soluble	1413:1419	arg1	best					1471:1474	best	1471:1474	best	1471:1474	Insoluble and soluble rye bran fractions and soluble oat fiber were the best for controlling the level of proteobacteria at the level below 2%.
31003566	6	41	theme	rye	1098:1100	arg1	fiber					1102:1106	rye fiber	1098:1106	rye fiber	1098:1106	The fermentation of rye bran, enzymatically treated rye bran, its insoluble fraction, soluble oat fiber and a mixture of rye fiber:soluble oat fiber:linseed resulted in a significant increase in butyrate production and a bifidogenic effect in the absence of the antibiotic.
31003566	4	42	theme	harmful	613:619	arg1	effects					621:627	the harmful effects	609:627	the harmful effects of amoxicillin-clavulanate on the microbiota	609:672	The model was also used to study whether fibers can alleviate the harmful effects of amoxicillin-clavulanate on the microbiota.
31003566	4	43	used	used	566:569	arg2	model					551:555	The model	547:555	The model	547:555	The model was also used to study whether fibers can alleviate the harmful effects of amoxicillin-clavulanate on the microbiota.
31003566	2	44	theme	dietary	361:367	arg1	fibers					369:374	potentially-prebiotic dietary fibers	339:374	potentially-prebiotic dietary fibers	339:374	We demonstrated the applicability of a mictotiter plate in the in vitro fermentation models for the screening of potentially-prebiotic dietary fibers.
31003566	5	45	from	community	723:731	arg1	absence					740:746	the absence	736:746	the absence of fibers	736:756	The antibiotic induced a shift in the bacterial community in the absence of fibers by decreasing the relative amounts of Bifidobacteriaceae, Bacteroidaceae, Prevotellaceae, Lachnospiraceae and Ruminococcaceae, and increasing proteobacterial Sutterilaceae levels from 1% to 11% of the total microbiota.
31003566	6	46	theme	mixture	1087:1093	arg1	fermentation					981:992	The fermentation	977:992	The fermentation of rye bran, enzymatically treated rye bran, its insoluble fraction, soluble oat fiber and a mixture of rye fiber:soluble oat fiber:linseed	977:1132	The fermentation of rye bran, enzymatically treated rye bran, its insoluble fraction, soluble oat fiber and a mixture of rye fiber:soluble oat fiber:linseed resulted in a significant increase in butyrate production and a bifidogenic effect in the absence of the antibiotic.
31003566	6	47	theme	antibiotic	1239:1248	arg1	absence					1224:1230	the absence	1220:1230	the absence of the antibiotic	1220:1248	The fermentation of rye bran, enzymatically treated rye bran, its insoluble fraction, soluble oat fiber and a mixture of rye fiber:soluble oat fiber:linseed resulted in a significant increase in butyrate production and a bifidogenic effect in the absence of the antibiotic.
31003566	2	48	theme	plate	276:280	arg1	applicability					246:258	the applicability	242:258	the applicability of a mictotiter plate in the in vitro fermentation models for the screening of potentially-prebiotic dietary fibers	242:374	We demonstrated the applicability of a mictotiter plate in the in vitro fermentation models for the screening of potentially-prebiotic dietary fibers.
31003566	1	49	theme	high-throughput	160:174	arg1	screening					176:184	fast high-throughput screening	155:184	fast high-throughput screening of their effects on the gut microbiota	155:223	The development of prebiotic fibers requires fast high-throughput screening of their effects on the gut microbiota.
31003566	3	50	link	linseed-derived	418:432	arg1	preparations					440:451	seven rye bran-, oat- and linseed-derived fiber preparations	392:451	seven rye bran-, oat- and linseed-derived fiber preparations	392:451	The effects of seven rye bran-, oat- and linseed-derived fiber preparations on the human fecal microbiota composition and short-chain fatty acid production were studied.
31003566	5	51	theme	relative	776:783	arg1	Ruminococcaceae					868:882	Ruminococcaceae	868:882	Ruminococcaceae	868:882	The antibiotic induced a shift in the bacterial community in the absence of fibers by decreasing the relative amounts of Bifidobacteriaceae, Bacteroidaceae, Prevotellaceae, Lachnospiraceae and Ruminococcaceae, and increasing proteobacterial Sutterilaceae levels from 1% to 11% of the total microbiota.
31003566	5	51	theme	relative	776:783	arg1	amounts					785:791	the relative amounts	772:791	the relative amounts of Bifidobacteriaceae, Bacteroidaceae, Prevotellaceae, Lachnospiraceae and Ruminococcaceae	772:882	The antibiotic induced a shift in the bacterial community in the absence of fibers by decreasing the relative amounts of Bifidobacteriaceae, Bacteroidaceae, Prevotellaceae, Lachnospiraceae and Ruminococcaceae, and increasing proteobacterial Sutterilaceae levels from 1% to 11% of the total microbiota.
31003566	5	51	theme	relative	776:783	arg1	Lachnospiraceae					848:862	Lachnospiraceae	848:862	Lachnospiraceae	848:862	The antibiotic induced a shift in the bacterial community in the absence of fibers by decreasing the relative amounts of Bifidobacteriaceae, Bacteroidaceae, Prevotellaceae, Lachnospiraceae and Ruminococcaceae, and increasing proteobacterial Sutterilaceae levels from 1% to 11% of the total microbiota.
31003566	5	51	theme	relative	776:783	arg1	Bacteroidaceae					816:829	Bacteroidaceae	816:829	Bacteroidaceae	816:829	The antibiotic induced a shift in the bacterial community in the absence of fibers by decreasing the relative amounts of Bifidobacteriaceae, Bacteroidaceae, Prevotellaceae, Lachnospiraceae and Ruminococcaceae, and increasing proteobacterial Sutterilaceae levels from 1% to 11% of the total microbiota.
31003566	5	52	theme	proteobacterial	900:914	arg1	levels					930:935	proteobacterial Sutterilaceae levels	900:935	proteobacterial Sutterilaceae levels	900:935	The antibiotic induced a shift in the bacterial community in the absence of fibers by decreasing the relative amounts of Bifidobacteriaceae, Bacteroidaceae, Prevotellaceae, Lachnospiraceae and Ruminococcaceae, and increasing proteobacterial Sutterilaceae levels from 1% to 11% of the total microbiota.
31003566	6	53	theme	rye	997:999	arg1	bran					1001:1004	rye bran	997:1004	rye bran	997:1004	The fermentation of rye bran, enzymatically treated rye bran, its insoluble fraction, soluble oat fiber and a mixture of rye fiber:soluble oat fiber:linseed resulted in a significant increase in butyrate production and a bifidogenic effect in the absence of the antibiotic.
31003566	7	54	theme	bifidobacteria	1383:1396	arg1	bifidobacteria					1383:1396	bifidobacteria	1383:1396	bifidobacteria	1383:1396	These fibers were also able to counteract the negative effects of the antibiotic and prevent the decrease in the relative amount of bifidobacteria.
31003566	7	54	theme	bifidobacteria	1383:1396	arg1	amount					1373:1378	the relative amount	1360:1378	the relative amount of bifidobacteria	1360:1396	These fibers were also able to counteract the negative effects of the antibiotic and prevent the decrease in the relative amount of bifidobacteria.
31003566	1	55	theme	effects	195:201	arg1	screening					176:184	fast high-throughput screening	155:184	fast high-throughput screening of their effects on the gut microbiota	155:223	The development of prebiotic fibers requires fast high-throughput screening of their effects on the gut microbiota.
31003566	8	56	theme	bran	1425:1428	arg1	fractions					1430:1438	Insoluble and soluble rye bran fractions	1399:1438	Insoluble and soluble rye bran fractions	1399:1438	Insoluble and soluble rye bran fractions and soluble oat fiber were the best for controlling the level of proteobacteria at the level below 2%.
31003566	8	56	theme	bran	1425:1428	arg1	fiber					1456:1460	soluble oat fiber	1444:1460	soluble oat fiber	1444:1460	Insoluble and soluble rye bran fractions and soluble oat fiber were the best for controlling the level of proteobacteria at the level below 2%.
31003566	8	56	theme	bran	1425:1428	arg1	best					1471:1474	best	1471:1474	best	1471:1474	Insoluble and soluble rye bran fractions and soluble oat fiber were the best for controlling the level of proteobacteria at the level below 2%.
31003566	5	57	theme	%	943:943	arg1	microbiota					965:974	the total microbiota	955:974	the total microbiota	955:974	The antibiotic induced a shift in the bacterial community in the absence of fibers by decreasing the relative amounts of Bifidobacteriaceae, Bacteroidaceae, Prevotellaceae, Lachnospiraceae and Ruminococcaceae, and increasing proteobacterial Sutterilaceae levels from 1% to 11% of the total microbiota.
31003566	5	57	theme	%	943:943	arg1	%					950:950	1% to 11%	942:950	1% to 11% of the total microbiota	942:974	The antibiotic induced a shift in the bacterial community in the absence of fibers by decreasing the relative amounts of Bifidobacteriaceae, Bacteroidaceae, Prevotellaceae, Lachnospiraceae and Ruminococcaceae, and increasing proteobacterial Sutterilaceae levels from 1% to 11% of the total microbiota.
31003566	5	58	theme	Ruminococcaceae	868:882	arg1	Ruminococcaceae					868:882	Ruminococcaceae	868:882	Ruminococcaceae	868:882	The antibiotic induced a shift in the bacterial community in the absence of fibers by decreasing the relative amounts of Bifidobacteriaceae, Bacteroidaceae, Prevotellaceae, Lachnospiraceae and Ruminococcaceae, and increasing proteobacterial Sutterilaceae levels from 1% to 11% of the total microbiota.
31003566	5	58	theme	Ruminococcaceae	868:882	arg1	amounts					785:791	the relative amounts	772:791	the relative amounts of Bifidobacteriaceae, Bacteroidaceae, Prevotellaceae, Lachnospiraceae and Ruminococcaceae	772:882	The antibiotic induced a shift in the bacterial community in the absence of fibers by decreasing the relative amounts of Bifidobacteriaceae, Bacteroidaceae, Prevotellaceae, Lachnospiraceae and Ruminococcaceae, and increasing proteobacterial Sutterilaceae levels from 1% to 11% of the total microbiota.
31003566	5	58	theme	Ruminococcaceae	868:882	arg1	Lachnospiraceae					848:862	Lachnospiraceae	848:862	Lachnospiraceae	848:862	The antibiotic induced a shift in the bacterial community in the absence of fibers by decreasing the relative amounts of Bifidobacteriaceae, Bacteroidaceae, Prevotellaceae, Lachnospiraceae and Ruminococcaceae, and increasing proteobacterial Sutterilaceae levels from 1% to 11% of the total microbiota.
31003566	5	58	theme	Ruminococcaceae	868:882	arg1	Bacteroidaceae					816:829	Bacteroidaceae	816:829	Bacteroidaceae	816:829	The antibiotic induced a shift in the bacterial community in the absence of fibers by decreasing the relative amounts of Bifidobacteriaceae, Bacteroidaceae, Prevotellaceae, Lachnospiraceae and Ruminococcaceae, and increasing proteobacterial Sutterilaceae levels from 1% to 11% of the total microbiota.
31003566	6	59	dep	fermentation	981:992	arg1	linseed					1126:1132	linseed	1126:1132	The fermentation of rye bran, enzymatically treated rye bran, its insoluble fraction, soluble oat fiber and a mixture of rye fiber:soluble oat fiber:linseed	977:1132	The fermentation of rye bran, enzymatically treated rye bran, its insoluble fraction, soluble oat fiber and a mixture of rye fiber:soluble oat fiber:linseed resulted in a significant increase in butyrate production and a bifidogenic effect in the absence of the antibiotic.
31003566	6	59	dep	fermentation	981:992	arg1	fiber					1120:1124	soluble oat fiber	1108:1124	The fermentation of rye bran, enzymatically treated rye bran, its insoluble fraction, soluble oat fiber and a mixture of rye fiber:soluble oat fiber:linseed	977:1132	The fermentation of rye bran, enzymatically treated rye bran, its insoluble fraction, soluble oat fiber and a mixture of rye fiber:soluble oat fiber:linseed resulted in a significant increase in butyrate production and a bifidogenic effect in the absence of the antibiotic.
31003566	6	60	theme	bifidogenic	1198:1208	arg1	effect					1210:1215	a bifidogenic effect	1196:1215	a bifidogenic effect in the absence of the antibiotic	1196:1248	The fermentation of rye bran, enzymatically treated rye bran, its insoluble fraction, soluble oat fiber and a mixture of rye fiber:soluble oat fiber:linseed resulted in a significant increase in butyrate production and a bifidogenic effect in the absence of the antibiotic.
31003566	2	61	dep	in	289:290	arg1	vitro					292:296	vitro	292:296	vitro	292:296	We demonstrated the applicability of a mictotiter plate in the in vitro fermentation models for the screening of potentially-prebiotic dietary fibers.
31003566	6	62	from	effect	1210:1215	arg1	absence					1224:1230	the absence	1220:1230	the absence of the antibiotic	1220:1248	The fermentation of rye bran, enzymatically treated rye bran, its insoluble fraction, soluble oat fiber and a mixture of rye fiber:soluble oat fiber:linseed resulted in a significant increase in butyrate production and a bifidogenic effect in the absence of the antibiotic.
31003566	6	62	from	effect	1210:1215	arg1	production					1181:1190	butyrate production	1172:1190	butyrate production	1172:1190	The fermentation of rye bran, enzymatically treated rye bran, its insoluble fraction, soluble oat fiber and a mixture of rye fiber:soluble oat fiber:linseed resulted in a significant increase in butyrate production and a bifidogenic effect in the absence of the antibiotic.
31003566	1	63	from	microbiota	214:223	arg1	screening					176:184	fast high-throughput screening	155:184	fast high-throughput screening of their effects on the gut microbiota	155:223	The development of prebiotic fibers requires fast high-throughput screening of their effects on the gut microbiota.
31003566	4	64	from	effects	621:627	arg1	microbiota					663:672	the microbiota	659:672	the microbiota	659:672	The model was also used to study whether fibers can alleviate the harmful effects of amoxicillin-clavulanate on the microbiota.
31003566	5	65	theme	Lachnospiraceae	848:862	arg1	Ruminococcaceae					868:882	Ruminococcaceae	868:882	Ruminococcaceae	868:882	The antibiotic induced a shift in the bacterial community in the absence of fibers by decreasing the relative amounts of Bifidobacteriaceae, Bacteroidaceae, Prevotellaceae, Lachnospiraceae and Ruminococcaceae, and increasing proteobacterial Sutterilaceae levels from 1% to 11% of the total microbiota.
31003566	5	65	theme	Lachnospiraceae	848:862	arg1	amounts					785:791	the relative amounts	772:791	the relative amounts of Bifidobacteriaceae, Bacteroidaceae, Prevotellaceae, Lachnospiraceae and Ruminococcaceae	772:882	The antibiotic induced a shift in the bacterial community in the absence of fibers by decreasing the relative amounts of Bifidobacteriaceae, Bacteroidaceae, Prevotellaceae, Lachnospiraceae and Ruminococcaceae, and increasing proteobacterial Sutterilaceae levels from 1% to 11% of the total microbiota.
31003566	5	65	theme	Lachnospiraceae	848:862	arg1	Lachnospiraceae					848:862	Lachnospiraceae	848:862	Lachnospiraceae	848:862	The antibiotic induced a shift in the bacterial community in the absence of fibers by decreasing the relative amounts of Bifidobacteriaceae, Bacteroidaceae, Prevotellaceae, Lachnospiraceae and Ruminococcaceae, and increasing proteobacterial Sutterilaceae levels from 1% to 11% of the total microbiota.
31003566	5	65	theme	Lachnospiraceae	848:862	arg1	Bacteroidaceae					816:829	Bacteroidaceae	816:829	Bacteroidaceae	816:829	The antibiotic induced a shift in the bacterial community in the absence of fibers by decreasing the relative amounts of Bifidobacteriaceae, Bacteroidaceae, Prevotellaceae, Lachnospiraceae and Ruminococcaceae, and increasing proteobacterial Sutterilaceae levels from 1% to 11% of the total microbiota.
31003566	1	66	theme	prebiotic	129:137	arg1	fibers					139:144	prebiotic fibers	129:144	prebiotic fibers	129:144	The development of prebiotic fibers requires fast high-throughput screening of their effects on the gut microbiota.
31003566	6	67	theme	bran	1033:1036	arg1	fermentation					981:992	The fermentation	977:992	The fermentation of rye bran, enzymatically treated rye bran, its insoluble fraction, soluble oat fiber and a mixture of rye fiber:soluble oat fiber:linseed	977:1132	The fermentation of rye bran, enzymatically treated rye bran, its insoluble fraction, soluble oat fiber and a mixture of rye fiber:soluble oat fiber:linseed resulted in a significant increase in butyrate production and a bifidogenic effect in the absence of the antibiotic.
31003566	7	68	theme	antibiotic	1321:1330	arg1	effects					1306:1312	the negative effects	1293:1312	the negative effects of the antibiotic	1293:1330	These fibers were also able to counteract the negative effects of the antibiotic and prevent the decrease in the relative amount of bifidobacteria.
31003566	1	69	from	screening	176:184	arg1	microbiota					214:223	the gut microbiota	206:223	the gut microbiota	206:223	The development of prebiotic fibers requires fast high-throughput screening of their effects on the gut microbiota.
31003566	6	70	theme	treated	1021:1027	arg1	bran					1033:1036	enzymatically treated rye bran	1007:1036	enzymatically treated rye bran	1007:1036	The fermentation of rye bran, enzymatically treated rye bran, its insoluble fraction, soluble oat fiber and a mixture of rye fiber:soluble oat fiber:linseed resulted in a significant increase in butyrate production and a bifidogenic effect in the absence of the antibiotic.
31003566	0	71	theme	Different	80:88	arg1	Preparations					96:107	Different Fiber Preparations	80:107	Different Fiber Preparations	80:107	A Small In Vitro Fermentation Model for Screening the Gut Microbiota Effects of Different Fiber Preparations.
31003566	0	72	theme	Preparations	96:107	arg1	Effects					69:75	the Gut Microbiota Effects	50:75	the Gut Microbiota Effects of Different Fiber Preparations	50:107	A Small In Vitro Fermentation Model for Screening the Gut Microbiota Effects of Different Fiber Preparations.
31003566	3	73	from	effects	381:387	arg1	production					522:531	short-chain fatty acid production	499:531	short-chain fatty acid production	499:531	The effects of seven rye bran-, oat- and linseed-derived fiber preparations on the human fecal microbiota composition and short-chain fatty acid production were studied.
31003566	3	73	from	effects	381:387	arg1	composition					483:493	the human fecal microbiota composition	456:493	the human fecal microbiota composition	456:493	The effects of seven rye bran-, oat- and linseed-derived fiber preparations on the human fecal microbiota composition and short-chain fatty acid production were studied.
31003566	8	74	theme	proteobacteria	1505:1518	arg1	level					1496:1500	the level	1492:1500	the level of proteobacteria	1492:1518	Insoluble and soluble rye bran fractions and soluble oat fiber were the best for controlling the level of proteobacteria at the level below 2%.
31003566	3	75	theme	preparations	440:451	arg1	effects					381:387	The effects	377:387	The effects of seven rye bran-, oat- and linseed-derived fiber preparations on the human fecal microbiota composition and short-chain fatty acid production	377:531	The effects of seven rye bran-, oat- and linseed-derived fiber preparations on the human fecal microbiota composition and short-chain fatty acid production were studied.
31003566	5	76	theme	Bacteroidaceae	816:829	arg1	Ruminococcaceae					868:882	Ruminococcaceae	868:882	Ruminococcaceae	868:882	The antibiotic induced a shift in the bacterial community in the absence of fibers by decreasing the relative amounts of Bifidobacteriaceae, Bacteroidaceae, Prevotellaceae, Lachnospiraceae and Ruminococcaceae, and increasing proteobacterial Sutterilaceae levels from 1% to 11% of the total microbiota.
31003566	5	76	theme	Bacteroidaceae	816:829	arg1	amounts					785:791	the relative amounts	772:791	the relative amounts of Bifidobacteriaceae, Bacteroidaceae, Prevotellaceae, Lachnospiraceae and Ruminococcaceae	772:882	The antibiotic induced a shift in the bacterial community in the absence of fibers by decreasing the relative amounts of Bifidobacteriaceae, Bacteroidaceae, Prevotellaceae, Lachnospiraceae and Ruminococcaceae, and increasing proteobacterial Sutterilaceae levels from 1% to 11% of the total microbiota.
31003566	5	76	theme	Bacteroidaceae	816:829	arg1	Lachnospiraceae					848:862	Lachnospiraceae	848:862	Lachnospiraceae	848:862	The antibiotic induced a shift in the bacterial community in the absence of fibers by decreasing the relative amounts of Bifidobacteriaceae, Bacteroidaceae, Prevotellaceae, Lachnospiraceae and Ruminococcaceae, and increasing proteobacterial Sutterilaceae levels from 1% to 11% of the total microbiota.
31003566	5	76	theme	Bacteroidaceae	816:829	arg1	Bacteroidaceae					816:829	Bacteroidaceae	816:829	Bacteroidaceae	816:829	The antibiotic induced a shift in the bacterial community in the absence of fibers by decreasing the relative amounts of Bifidobacteriaceae, Bacteroidaceae, Prevotellaceae, Lachnospiraceae and Ruminococcaceae, and increasing proteobacterial Sutterilaceae levels from 1% to 11% of the total microbiota.
31003566	6	77	theme	fiber	1075:1079	arg1	fermentation					981:992	The fermentation	977:992	The fermentation of rye bran, enzymatically treated rye bran, its insoluble fraction, soluble oat fiber and a mixture of rye fiber:soluble oat fiber:linseed	977:1132	The fermentation of rye bran, enzymatically treated rye bran, its insoluble fraction, soluble oat fiber and a mixture of rye fiber:soluble oat fiber:linseed resulted in a significant increase in butyrate production and a bifidogenic effect in the absence of the antibiotic.
31003566	3	78	theme	linseed-derived	418:432	arg1	preparations					440:451	seven rye bran-, oat- and linseed-derived fiber preparations	392:451	seven rye bran-, oat- and linseed-derived fiber preparations	392:451	The effects of seven rye bran-, oat- and linseed-derived fiber preparations on the human fecal microbiota composition and short-chain fatty acid production were studied.
31003566	5	79	theme	microbiota	965:974	arg1	microbiota					965:974	the total microbiota	955:974	the total microbiota	955:974	The antibiotic induced a shift in the bacterial community in the absence of fibers by decreasing the relative amounts of Bifidobacteriaceae, Bacteroidaceae, Prevotellaceae, Lachnospiraceae and Ruminococcaceae, and increasing proteobacterial Sutterilaceae levels from 1% to 11% of the total microbiota.
31003566	5	79	theme	microbiota	965:974	arg1	%					950:950	1% to 11%	942:950	1% to 11% of the total microbiota	942:974	The antibiotic induced a shift in the bacterial community in the absence of fibers by decreasing the relative amounts of Bifidobacteriaceae, Bacteroidaceae, Prevotellaceae, Lachnospiraceae and Ruminococcaceae, and increasing proteobacterial Sutterilaceae levels from 1% to 11% of the total microbiota.
31003566	7	80	from	decrease	1348:1355	arg1	bifidobacteria					1383:1396	bifidobacteria	1383:1396	bifidobacteria	1383:1396	These fibers were also able to counteract the negative effects of the antibiotic and prevent the decrease in the relative amount of bifidobacteria.
31003566	7	80	from	decrease	1348:1355	arg1	amount					1373:1378	the relative amount	1360:1378	the relative amount of bifidobacteria	1360:1396	These fibers were also able to counteract the negative effects of the antibiotic and prevent the decrease in the relative amount of bifidobacteria.
31003566	6	81	theme	soluble	1063:1069	arg1	fiber					1075:1079	soluble oat fiber	1063:1079	soluble oat fiber	1063:1079	The fermentation of rye bran, enzymatically treated rye bran, its insoluble fraction, soluble oat fiber and a mixture of rye fiber:soluble oat fiber:linseed resulted in a significant increase in butyrate production and a bifidogenic effect in the absence of the antibiotic.
31003566	3	82	theme	fecal	466:470	arg1	composition					483:493	the human fecal microbiota composition	456:493	the human fecal microbiota composition	456:493	The effects of seven rye bran-, oat- and linseed-derived fiber preparations on the human fecal microbiota composition and short-chain fatty acid production were studied.
31003566	6	83	theme	fraction	1053:1060	arg1	fermentation					981:992	The fermentation	977:992	The fermentation of rye bran, enzymatically treated rye bran, its insoluble fraction, soluble oat fiber and a mixture of rye fiber:soluble oat fiber:linseed	977:1132	The fermentation of rye bran, enzymatically treated rye bran, its insoluble fraction, soluble oat fiber and a mixture of rye fiber:soluble oat fiber:linseed resulted in a significant increase in butyrate production and a bifidogenic effect in the absence of the antibiotic.
31003566	3	84	theme	oat-	409:412	arg1	preparations					440:451	seven rye bran-, oat- and linseed-derived fiber preparations	392:451	seven rye bran-, oat- and linseed-derived fiber preparations	392:451	The effects of seven rye bran-, oat- and linseed-derived fiber preparations on the human fecal microbiota composition and short-chain fatty acid production were studied.
31003566	0	85	theme	Fiber	90:94	arg1	Preparations					96:107	Different Fiber Preparations	80:107	Different Fiber Preparations	80:107	A Small In Vitro Fermentation Model for Screening the Gut Microbiota Effects of Different Fiber Preparations.
31003566	3	86	theme	bran-	402:406	arg1	preparations					440:451	seven rye bran-, oat- and linseed-derived fiber preparations	392:451	seven rye bran-, oat- and linseed-derived fiber preparations	392:451	The effects of seven rye bran-, oat- and linseed-derived fiber preparations on the human fecal microbiota composition and short-chain fatty acid production were studied.
31003566	3	87	theme	acid	517:520	arg1	production					522:531	short-chain fatty acid production	499:531	short-chain fatty acid production	499:531	The effects of seven rye bran-, oat- and linseed-derived fiber preparations on the human fecal microbiota composition and short-chain fatty acid production were studied.
31003566	3	88	theme	short-chain	499:509	arg1	acid					517:520	short-chain fatty acid	499:520	short-chain fatty acid production	499:531	The effects of seven rye bran-, oat- and linseed-derived fiber preparations on the human fecal microbiota composition and short-chain fatty acid production were studied.
31003566	6	89	theme	soluble	1108:1114	arg1	fiber					1120:1124	soluble oat fiber	1108:1124	The fermentation of rye bran, enzymatically treated rye bran, its insoluble fraction, soluble oat fiber and a mixture of rye fiber:soluble oat fiber:linseed	977:1132	The fermentation of rye bran, enzymatically treated rye bran, its insoluble fraction, soluble oat fiber and a mixture of rye fiber:soluble oat fiber:linseed resulted in a significant increase in butyrate production and a bifidogenic effect in the absence of the antibiotic.
31003566	2	90	theme	fibers	369:374	arg1	screening					326:334	the screening	322:334	the screening of potentially-prebiotic dietary fibers	322:374	We demonstrated the applicability of a mictotiter plate in the in vitro fermentation models for the screening of potentially-prebiotic dietary fibers.
31003566	6	91	theme	fiber	1102:1106	arg1	fiber					1075:1079	soluble oat fiber	1063:1079	soluble oat fiber	1063:1079	The fermentation of rye bran, enzymatically treated rye bran, its insoluble fraction, soluble oat fiber and a mixture of rye fiber:soluble oat fiber:linseed resulted in a significant increase in butyrate production and a bifidogenic effect in the absence of the antibiotic.
31003566	6	91	theme	fiber	1102:1106	arg1	bran					1033:1036	enzymatically treated rye bran	1007:1036	enzymatically treated rye bran	1007:1036	The fermentation of rye bran, enzymatically treated rye bran, its insoluble fraction, soluble oat fiber and a mixture of rye fiber:soluble oat fiber:linseed resulted in a significant increase in butyrate production and a bifidogenic effect in the absence of the antibiotic.
31003566	6	91	theme	fiber	1102:1106	arg1	bran					1001:1004	rye bran	997:1004	rye bran	997:1004	The fermentation of rye bran, enzymatically treated rye bran, its insoluble fraction, soluble oat fiber and a mixture of rye fiber:soluble oat fiber:linseed resulted in a significant increase in butyrate production and a bifidogenic effect in the absence of the antibiotic.
31003566	6	91	theme	fiber	1102:1106	arg1	fraction					1053:1060	its insoluble fraction	1039:1060	its insoluble fraction	1039:1060	The fermentation of rye bran, enzymatically treated rye bran, its insoluble fraction, soluble oat fiber and a mixture of rye fiber:soluble oat fiber:linseed resulted in a significant increase in butyrate production and a bifidogenic effect in the absence of the antibiotic.
31003566	6	91	theme	fiber	1102:1106	arg1	mixture					1087:1093	a mixture	1085:1093	a mixture of rye fiber	1085:1106	The fermentation of rye bran, enzymatically treated rye bran, its insoluble fraction, soluble oat fiber and a mixture of rye fiber:soluble oat fiber:linseed resulted in a significant increase in butyrate production and a bifidogenic effect in the absence of the antibiotic.
31003566	0	92	theme	Small	2:6	arg1	Model					30:34	A Small In Vitro Fermentation Model	0:34	A Small In Vitro Fermentation Model for Screening the Gut Microbiota Effects of Different Fiber Preparations	0:107	A Small In Vitro Fermentation Model for Screening the Gut Microbiota Effects of Different Fiber Preparations.
31609624	2	0	theme	body	600:603	arg1	distribution					605:616	the cellular and body distribution	583:616	distribution	605:616	We now investigated the physicochemical mechanisms underlying these different behaviors of NP variations at biological barriers and their influence on the cellular and body distribution.
31609624	3	1	theme	retina	712:717	arg1	imaging					719:725	retina imaging	712:725	retina imaging ex vivo	712:733	Retinal whole mounts from rats injected in vivo with fluorescent PBCA NPs were processed for retina imaging ex vivo to obtain a detailed distribution of NPs with cellular resolution in retinal tissue.
31609624	1	2	theme	poloxamer	151:159	arg1	composition					136:146	the composition	132:146	the composition of poloxamer 188-modified, DEAE-dextran-stabilized (PDD) polybutylcyanoacrylate (PBCA) nanoparticles (NPs)	132:253	Minor changes in the composition of poloxamer 188-modified, DEAE-dextran-stabilized (PDD) polybutylcyanoacrylate (PBCA) nanoparticles (NPs), by altering the physicochemical parameters (such as size or surface charge), can substantially influence their delivery kinetics across the blood-retina barrier (BRB) in vivo.
31609624	4	3	theme	retinal	1000:1006	arg1	cells					1017:1021	retinal ganglion cells	1000:1021	retinal ganglion cells (RGCs)	1000:1028	In line with previous in vivo imaging results, NPs with a larger size and medium surface charge accumulated more readily in brain tissue, and they could be more easily detected in retinal ganglion cells (RGCs), demonstrating the potential of these NPs for drug delivery into neurons.
31609624	4	3	theme	retinal	1000:1006	arg1	RGCs					1024:1027	RGCs	1024:1027	RGCs	1024:1027	In line with previous in vivo imaging results, NPs with a larger size and medium surface charge accumulated more readily in brain tissue, and they could be more easily detected in retinal ganglion cells (RGCs), demonstrating the potential of these NPs for drug delivery into neurons.
31609624	7	4	theme	minor	1456:1460	arg1	changes					1462:1468	minor changes	1456:1468	minor changes of NPs' physicochemical parameters	1456:1503	In this way, minor changes of NPs' physicochemical parameters can significantly impact their rate of brain/body biodistribution.
31609624	2	5	theme	physicochemical	456:470	arg1	mechanisms					472:481	the physicochemical mechanisms	452:481	the physicochemical mechanisms underlying these different behaviors of NP variations at biological barriers	452:558	We now investigated the physicochemical mechanisms underlying these different behaviors of NP variations at biological barriers and their influence on the cellular and body distribution.
31609624	3	6	theme	PBCA	684:687	arg1	NPs					689:691	fluorescent PBCA NPs	672:691	fluorescent PBCA NPs	672:691	Retinal whole mounts from rats injected in vivo with fluorescent PBCA NPs were processed for retina imaging ex vivo to obtain a detailed distribution of NPs with cellular resolution in retinal tissue.
31609624	3	7	from	distribution	756:767	arg1	tissue					812:817	retinal tissue	804:817	retinal tissue	804:817	Retinal whole mounts from rats injected in vivo with fluorescent PBCA NPs were processed for retina imaging ex vivo to obtain a detailed distribution of NPs with cellular resolution in retinal tissue.
31609624	1	8	theme	188-modified	161:172	arg1	poloxamer					151:159	poloxamer 188-modified	151:172	poloxamer 188-modified	151:172	Minor changes in the composition of poloxamer 188-modified, DEAE-dextran-stabilized (PDD) polybutylcyanoacrylate (PBCA) nanoparticles (NPs), by altering the physicochemical parameters (such as size or surface charge), can substantially influence their delivery kinetics across the blood-retina barrier (BRB) in vivo.
31609624	1	8	theme	188-modified	161:172	arg1	nanoparticles					235:247	DEAE-dextran-stabilized (PDD) polybutylcyanoacrylate (PBCA) nanoparticles	175:247	DEAE-dextran-stabilized (PDD) polybutylcyanoacrylate (PBCA) nanoparticles (NPs)	175:253	Minor changes in the composition of poloxamer 188-modified, DEAE-dextran-stabilized (PDD) polybutylcyanoacrylate (PBCA) nanoparticles (NPs), by altering the physicochemical parameters (such as size or surface charge), can substantially influence their delivery kinetics across the blood-retina barrier (BRB) in vivo.
31609624	2	9	from	mechanisms	472:481	arg1	distribution					605:616	the cellular and body distribution	583:616	distribution	605:616	We now investigated the physicochemical mechanisms underlying these different behaviors of NP variations at biological barriers and their influence on the cellular and body distribution.
31609624	0	10	theme	Systemic	92:99	arg1	Interactions					101:112	Systemic Interactions	92:112	Systemic Interactions	92:112	How Nanoparticle Physicochemical Parameters Affect Drug Delivery to Cells in the Retina via Systemic Interactions.
31609624	2	11	theme	cellular	587:594	arg1	distribution					605:616	the cellular and body distribution	583:616	distribution	605:616	We now investigated the physicochemical mechanisms underlying these different behaviors of NP variations at biological barriers and their influence on the cellular and body distribution.
31609624	4	12	with	line	823:826	arg1	results					858:864	previous in vivo imaging results	833:864	previous in vivo imaging results	833:864	In line with previous in vivo imaging results, NPs with a larger size and medium surface charge accumulated more readily in brain tissue, and they could be more easily detected in retinal ganglion cells (RGCs), demonstrating the potential of these NPs for drug delivery into neurons.
31609624	3	13	theme	retinal	804:810	arg1	tissue					812:817	retinal tissue	804:817	retinal tissue	804:817	Retinal whole mounts from rats injected in vivo with fluorescent PBCA NPs were processed for retina imaging ex vivo to obtain a detailed distribution of NPs with cellular resolution in retinal tissue.
31609624	7	14	from	impact	1523:1528	arg1	way					1451:1453	this way	1446:1453	this way	1446:1453	In this way, minor changes of NPs' physicochemical parameters can significantly impact their rate of brain/body biodistribution.
31609624	4	15	located	detected	988:995	arg1	cells					1017:1021	retinal ganglion cells	1000:1021	retinal ganglion cells (RGCs)	1000:1028	In line with previous in vivo imaging results, NPs with a larger size and medium surface charge accumulated more readily in brain tissue, and they could be more easily detected in retinal ganglion cells (RGCs), demonstrating the potential of these NPs for drug delivery into neurons.
31609624	4	15	located	detected	988:995	arg1	RGCs					1024:1027	RGCs	1024:1027	RGCs	1024:1027	In line with previous in vivo imaging results, NPs with a larger size and medium surface charge accumulated more readily in brain tissue, and they could be more easily detected in retinal ganglion cells (RGCs), demonstrating the potential of these NPs for drug delivery into neurons.
31609624	4	15	located	detected	988:995	arg2	they					962:965	they	962:965	they	962:965	In line with previous in vivo imaging results, NPs with a larger size and medium surface charge accumulated more readily in brain tissue, and they could be more easily detected in retinal ganglion cells (RGCs), demonstrating the potential of these NPs for drug delivery into neurons.
31609624	7	16	theme	biodistribution	1555:1569	arg1	rate					1536:1539	their rate	1530:1539	their rate of brain/body biodistribution	1530:1569	In this way, minor changes of NPs' physicochemical parameters can significantly impact their rate of brain/body biodistribution.
31609624	5	17	theme	brain	1260:1264	arg1	tissue					1266:1271	brain tissue	1260:1271	brain tissue	1260:1271	The biodistribution of the NPs revealed a higher accumulation of small-sized NPs in peripheral organs, which may reduce the passage of these particles into brain tissue via a "steal effect" mechanism.
31609624	2	18	from	influence	570:578	arg1	distribution					605:616	the cellular and body distribution	583:616	distribution	605:616	We now investigated the physicochemical mechanisms underlying these different behaviors of NP variations at biological barriers and their influence on the cellular and body distribution.
31609624	3	19	from	rats	645:648	arg1	mounts					633:638	Retinal whole mounts	619:638	Retinal whole mounts from rats injected in vivo with fluorescent PBCA NPs	619:691	Retinal whole mounts from rats injected in vivo with fluorescent PBCA NPs were processed for retina imaging ex vivo to obtain a detailed distribution of NPs with cellular resolution in retinal tissue.
31609624	3	20	theme	ex	727:728	arg1	imaging					719:725	retina imaging	712:725	retina imaging ex vivo	712:733	Retinal whole mounts from rats injected in vivo with fluorescent PBCA NPs were processed for retina imaging ex vivo to obtain a detailed distribution of NPs with cellular resolution in retinal tissue.
31609624	3	21	with	NPs	772:774	arg1	resolution					790:799	cellular resolution	781:799	cellular resolution	781:799	Retinal whole mounts from rats injected in vivo with fluorescent PBCA NPs were processed for retina imaging ex vivo to obtain a detailed distribution of NPs with cellular resolution in retinal tissue.
31609624	4	22	theme	NPs	1068:1070	arg1	potential					1049:1057	the potential	1045:1057	the potential of these NPs for drug delivery into neurons	1045:1101	In line with previous in vivo imaging results, NPs with a larger size and medium surface charge accumulated more readily in brain tissue, and they could be more easily detected in retinal ganglion cells (RGCs), demonstrating the potential of these NPs for drug delivery into neurons.
31609624	4	23	theme	imaging	850:856	arg1	results					858:864	previous in vivo imaging results	833:864	previous in vivo imaging results	833:864	In line with previous in vivo imaging results, NPs with a larger size and medium surface charge accumulated more readily in brain tissue, and they could be more easily detected in retinal ganglion cells (RGCs), demonstrating the potential of these NPs for drug delivery into neurons.
31609624	2	24	theme	different	500:508	arg1	behaviors					510:518	these different behaviors	494:518	these different behaviors of NP variations at biological barriers	494:558	We now investigated the physicochemical mechanisms underlying these different behaviors of NP variations at biological barriers and their influence on the cellular and body distribution.
31609624	7	25	theme	physicochemical	1478:1492	arg1	parameters					1494:1503	NPs' physicochemical parameters	1473:1503	NPs' physicochemical parameters	1473:1503	In this way, minor changes of NPs' physicochemical parameters can significantly impact their rate of brain/body biodistribution.
31609624	1	26	theme	DEAE-dextran-stabilized	175:197	arg1	NPs					250:252	NPs	250:252	NPs	250:252	Minor changes in the composition of poloxamer 188-modified, DEAE-dextran-stabilized (PDD) polybutylcyanoacrylate (PBCA) nanoparticles (NPs), by altering the physicochemical parameters (such as size or surface charge), can substantially influence their delivery kinetics across the blood-retina barrier (BRB) in vivo.
31609624	1	26	theme	DEAE-dextran-stabilized	175:197	arg1	poloxamer					151:159	poloxamer 188-modified	151:172	poloxamer 188-modified	151:172	Minor changes in the composition of poloxamer 188-modified, DEAE-dextran-stabilized (PDD) polybutylcyanoacrylate (PBCA) nanoparticles (NPs), by altering the physicochemical parameters (such as size or surface charge), can substantially influence their delivery kinetics across the blood-retina barrier (BRB) in vivo.
31609624	1	26	theme	DEAE-dextran-stabilized	175:197	arg1	nanoparticles					235:247	DEAE-dextran-stabilized (PDD) polybutylcyanoacrylate (PBCA) nanoparticles	175:247	DEAE-dextran-stabilized (PDD) polybutylcyanoacrylate (PBCA) nanoparticles (NPs)	175:253	Minor changes in the composition of poloxamer 188-modified, DEAE-dextran-stabilized (PDD) polybutylcyanoacrylate (PBCA) nanoparticles (NPs), by altering the physicochemical parameters (such as size or surface charge), can substantially influence their delivery kinetics across the blood-retina barrier (BRB) in vivo.
31609624	0	27	theme	Nanoparticle	4:15	arg1	Parameters					33:42	Nanoparticle Physicochemical Parameters	4:42	Nanoparticle Physicochemical Parameters	4:42	How Nanoparticle Physicochemical Parameters Affect Drug Delivery to Cells in the Retina via Systemic Interactions.
31609624	1	28	from	barrier	409:415	arg1	vivo					426:429	vivo	426:429	vivo	426:429	Minor changes in the composition of poloxamer 188-modified, DEAE-dextran-stabilized (PDD) polybutylcyanoacrylate (PBCA) nanoparticles (NPs), by altering the physicochemical parameters (such as size or surface charge), can substantially influence their delivery kinetics across the blood-retina barrier (BRB) in vivo.
31609624	4	29	theme	in	842:843	arg1	results					858:864	previous in vivo imaging results	833:864	previous in vivo imaging results	833:864	In line with previous in vivo imaging results, NPs with a larger size and medium surface charge accumulated more readily in brain tissue, and they could be more easily detected in retinal ganglion cells (RGCs), demonstrating the potential of these NPs for drug delivery into neurons.
31609624	4	30	theme	drug	1076:1079	arg1	delivery					1081:1088	drug delivery	1076:1088	drug delivery into neurons	1076:1101	In line with previous in vivo imaging results, NPs with a larger size and medium surface charge accumulated more readily in brain tissue, and they could be more easily detected in retinal ganglion cells (RGCs), demonstrating the potential of these NPs for drug delivery into neurons.
31609624	4	31	theme	previous	833:840	arg1	results					858:864	previous in vivo imaging results	833:864	previous in vivo imaging results	833:864	In line with previous in vivo imaging results, NPs with a larger size and medium surface charge accumulated more readily in brain tissue, and they could be more easily detected in retinal ganglion cells (RGCs), demonstrating the potential of these NPs for drug delivery into neurons.
31609624	2	32	from	behaviors	510:518	arg1	barriers					551:558	biological barriers	540:558	biological barriers	540:558	We now investigated the physicochemical mechanisms underlying these different behaviors of NP variations at biological barriers and their influence on the cellular and body distribution.
31609624	5	33	from	accumulation	1153:1164	arg1	organs					1199:1204	peripheral organs	1188:1204	peripheral organs	1188:1204	The biodistribution of the NPs revealed a higher accumulation of small-sized NPs in peripheral organs, which may reduce the passage of these particles into brain tissue via a "steal effect" mechanism.
31609624	1	34	theme	PDD	200:202	arg1	NPs					250:252	NPs	250:252	NPs	250:252	Minor changes in the composition of poloxamer 188-modified, DEAE-dextran-stabilized (PDD) polybutylcyanoacrylate (PBCA) nanoparticles (NPs), by altering the physicochemical parameters (such as size or surface charge), can substantially influence their delivery kinetics across the blood-retina barrier (BRB) in vivo.
31609624	1	34	theme	PDD	200:202	arg1	poloxamer					151:159	poloxamer 188-modified	151:172	poloxamer 188-modified	151:172	Minor changes in the composition of poloxamer 188-modified, DEAE-dextran-stabilized (PDD) polybutylcyanoacrylate (PBCA) nanoparticles (NPs), by altering the physicochemical parameters (such as size or surface charge), can substantially influence their delivery kinetics across the blood-retina barrier (BRB) in vivo.
31609624	1	34	theme	PDD	200:202	arg1	nanoparticles					235:247	DEAE-dextran-stabilized (PDD) polybutylcyanoacrylate (PBCA) nanoparticles	175:247	DEAE-dextran-stabilized (PDD) polybutylcyanoacrylate (PBCA) nanoparticles (NPs)	175:253	Minor changes in the composition of poloxamer 188-modified, DEAE-dextran-stabilized (PDD) polybutylcyanoacrylate (PBCA) nanoparticles (NPs), by altering the physicochemical parameters (such as size or surface charge), can substantially influence their delivery kinetics across the blood-retina barrier (BRB) in vivo.
31609624	1	35	theme	physicochemical	272:286	arg1	parameters					288:297	the physicochemical parameters	268:297	the physicochemical parameters (such as size or surface charge)	268:330	Minor changes in the composition of poloxamer 188-modified, DEAE-dextran-stabilized (PDD) polybutylcyanoacrylate (PBCA) nanoparticles (NPs), by altering the physicochemical parameters (such as size or surface charge), can substantially influence their delivery kinetics across the blood-retina barrier (BRB) in vivo.
31609624	1	36	from	changes	121:127	arg1	composition					136:146	the composition	132:146	the composition of poloxamer 188-modified, DEAE-dextran-stabilized (PDD) polybutylcyanoacrylate (PBCA) nanoparticles (NPs)	132:253	Minor changes in the composition of poloxamer 188-modified, DEAE-dextran-stabilized (PDD) polybutylcyanoacrylate (PBCA) nanoparticles (NPs), by altering the physicochemical parameters (such as size or surface charge), can substantially influence their delivery kinetics across the blood-retina barrier (BRB) in vivo.
31609624	0	37	theme	Physicochemical	17:31	arg1	Parameters					33:42	Nanoparticle Physicochemical Parameters	4:42	Nanoparticle Physicochemical Parameters	4:42	How Nanoparticle Physicochemical Parameters Affect Drug Delivery to Cells in the Retina via Systemic Interactions.
31609624	2	38	theme	biological	540:549	arg1	barriers					551:558	biological barriers	540:558	biological barriers	540:558	We now investigated the physicochemical mechanisms underlying these different behaviors of NP variations at biological barriers and their influence on the cellular and body distribution.
31609624	1	39	theme	delivery	367:374	arg1	kinetics					376:383	their delivery kinetics	361:383	their delivery kinetics	361:383	Minor changes in the composition of poloxamer 188-modified, DEAE-dextran-stabilized (PDD) polybutylcyanoacrylate (PBCA) nanoparticles (NPs), by altering the physicochemical parameters (such as size or surface charge), can substantially influence their delivery kinetics across the blood-retina barrier (BRB) in vivo.
31609624	6	40	theme	nervous	1421:1427	arg1	CNS					1437:1439	CNS	1437:1439	CNS	1437:1439	Thus, systemic interactions significantly determine the potential of NPs to deliver markers or drugs to the central nervous system (CNS).
31609624	6	40	theme	nervous	1421:1427	arg1	system					1429:1434	the central nervous system	1409:1434	the central nervous system (CNS)	1409:1440	Thus, systemic interactions significantly determine the potential of NPs to deliver markers or drugs to the central nervous system (CNS).
31609624	5	41	theme	particles	1245:1253	arg1	passage					1228:1234	the passage	1224:1234	the passage of these particles into brain tissue	1224:1271	The biodistribution of the NPs revealed a higher accumulation of small-sized NPs in peripheral organs, which may reduce the passage of these particles into brain tissue via a "steal effect" mechanism.
31609624	1	42	theme	polybutylcyanoacrylate	205:226	arg1	NPs					250:252	NPs	250:252	NPs	250:252	Minor changes in the composition of poloxamer 188-modified, DEAE-dextran-stabilized (PDD) polybutylcyanoacrylate (PBCA) nanoparticles (NPs), by altering the physicochemical parameters (such as size or surface charge), can substantially influence their delivery kinetics across the blood-retina barrier (BRB) in vivo.
31609624	1	42	theme	polybutylcyanoacrylate	205:226	arg1	poloxamer					151:159	poloxamer 188-modified	151:172	poloxamer 188-modified	151:172	Minor changes in the composition of poloxamer 188-modified, DEAE-dextran-stabilized (PDD) polybutylcyanoacrylate (PBCA) nanoparticles (NPs), by altering the physicochemical parameters (such as size or surface charge), can substantially influence their delivery kinetics across the blood-retina barrier (BRB) in vivo.
31609624	1	42	theme	polybutylcyanoacrylate	205:226	arg1	nanoparticles					235:247	DEAE-dextran-stabilized (PDD) polybutylcyanoacrylate (PBCA) nanoparticles	175:247	DEAE-dextran-stabilized (PDD) polybutylcyanoacrylate (PBCA) nanoparticles (NPs)	175:253	Minor changes in the composition of poloxamer 188-modified, DEAE-dextran-stabilized (PDD) polybutylcyanoacrylate (PBCA) nanoparticles (NPs), by altering the physicochemical parameters (such as size or surface charge), can substantially influence their delivery kinetics across the blood-retina barrier (BRB) in vivo.
31609624	0	43	theme	Drug	51:54	arg1	Delivery					56:63	Drug Delivery	51:63	Drug Delivery to Cells	51:72	How Nanoparticle Physicochemical Parameters Affect Drug Delivery to Cells in the Retina via Systemic Interactions.
31609624	5	44	theme	NPs	1131:1133	arg1	biodistribution					1108:1122	The biodistribution	1104:1122	The biodistribution of the NPs	1104:1133	The biodistribution of the NPs revealed a higher accumulation of small-sized NPs in peripheral organs, which may reduce the passage of these particles into brain tissue via a "steal effect" mechanism.
31609624	4	45	theme	surface	901:907	arg1	charge					909:914	medium surface charge	894:914	medium surface charge	894:914	In line with previous in vivo imaging results, NPs with a larger size and medium surface charge accumulated more readily in brain tissue, and they could be more easily detected in retinal ganglion cells (RGCs), demonstrating the potential of these NPs for drug delivery into neurons.
31609624	6	46	theme	NPs	1374:1376	arg1	potential					1361:1369	the potential	1357:1369	the potential of NPs to deliver markers or drugs to the central nervous system (CNS)	1357:1440	Thus, systemic interactions significantly determine the potential of NPs to deliver markers or drugs to the central nervous system (CNS).
31609624	4	47	with	NPs	867:869	arg1	charge					909:914	medium surface charge	894:914	medium surface charge	894:914	In line with previous in vivo imaging results, NPs with a larger size and medium surface charge accumulated more readily in brain tissue, and they could be more easily detected in retinal ganglion cells (RGCs), demonstrating the potential of these NPs for drug delivery into neurons.
31609624	4	47	with	NPs	867:869	arg1	size					885:888	a larger size	876:888	a larger size	876:888	In line with previous in vivo imaging results, NPs with a larger size and medium surface charge accumulated more readily in brain tissue, and they could be more easily detected in retinal ganglion cells (RGCs), demonstrating the potential of these NPs for drug delivery into neurons.
31609624	5	48	theme	steal	1280:1284	arg1	"					1292:1292	a "steal effect"	1277:1292	a "steal effect" mechanism	1277:1302	The biodistribution of the NPs revealed a higher accumulation of small-sized NPs in peripheral organs, which may reduce the passage of these particles into brain tissue via a "steal effect" mechanism.
31609624	2	49	theme	variations	526:535	arg1	behaviors					510:518	these different behaviors	494:518	these different behaviors of NP variations at biological barriers	494:558	We now investigated the physicochemical mechanisms underlying these different behaviors of NP variations at biological barriers and their influence on the cellular and body distribution.
31609624	5	50	theme	NPs	1181:1183	arg1	accumulation					1153:1164	a higher accumulation	1144:1164	a higher accumulation of small-sized NPs in peripheral organs, which may reduce the passage of these particles into brain tissue via a "steal effect" mechanism	1144:1302	The biodistribution of the NPs revealed a higher accumulation of small-sized NPs in peripheral organs, which may reduce the passage of these particles into brain tissue via a "steal effect" mechanism.
31609624	1	51	theme	PBCA	229:232	arg1	NPs					250:252	NPs	250:252	NPs	250:252	Minor changes in the composition of poloxamer 188-modified, DEAE-dextran-stabilized (PDD) polybutylcyanoacrylate (PBCA) nanoparticles (NPs), by altering the physicochemical parameters (such as size or surface charge), can substantially influence their delivery kinetics across the blood-retina barrier (BRB) in vivo.
31609624	1	51	theme	PBCA	229:232	arg1	poloxamer					151:159	poloxamer 188-modified	151:172	poloxamer 188-modified	151:172	Minor changes in the composition of poloxamer 188-modified, DEAE-dextran-stabilized (PDD) polybutylcyanoacrylate (PBCA) nanoparticles (NPs), by altering the physicochemical parameters (such as size or surface charge), can substantially influence their delivery kinetics across the blood-retina barrier (BRB) in vivo.
31609624	1	51	theme	PBCA	229:232	arg1	nanoparticles					235:247	DEAE-dextran-stabilized (PDD) polybutylcyanoacrylate (PBCA) nanoparticles	175:247	DEAE-dextran-stabilized (PDD) polybutylcyanoacrylate (PBCA) nanoparticles (NPs)	175:253	Minor changes in the composition of poloxamer 188-modified, DEAE-dextran-stabilized (PDD) polybutylcyanoacrylate (PBCA) nanoparticles (NPs), by altering the physicochemical parameters (such as size or surface charge), can substantially influence their delivery kinetics across the blood-retina barrier (BRB) in vivo.
31609624	2	52	theme	NP	523:524	arg1	variations					526:535	NP variations	523:535	NP variations at biological barriers	523:558	We now investigated the physicochemical mechanisms underlying these different behaviors of NP variations at biological barriers and their influence on the cellular and body distribution.
31609624	7	53	theme	brain/body	1544:1553	arg1	biodistribution					1555:1569	brain/body biodistribution	1544:1569	brain/body biodistribution	1544:1569	In this way, minor changes of NPs' physicochemical parameters can significantly impact their rate of brain/body biodistribution.
31609624	1	54	theme	Minor	115:119	arg1	changes					121:127	Minor changes	115:127	Minor changes in the composition of poloxamer 188-modified, DEAE-dextran-stabilized (PDD) polybutylcyanoacrylate (PBCA) nanoparticles (NPs)	115:253	Minor changes in the composition of poloxamer 188-modified, DEAE-dextran-stabilized (PDD) polybutylcyanoacrylate (PBCA) nanoparticles (NPs), by altering the physicochemical parameters (such as size or surface charge), can substantially influence their delivery kinetics across the blood-retina barrier (BRB) in vivo.
31609624	4	55	theme	medium	894:899	arg1	charge					909:914	medium surface charge	894:914	medium surface charge	894:914	In line with previous in vivo imaging results, NPs with a larger size and medium surface charge accumulated more readily in brain tissue, and they could be more easily detected in retinal ganglion cells (RGCs), demonstrating the potential of these NPs for drug delivery into neurons.
31609624	3	56	theme	detailed	747:754	arg1	distribution					756:767	a detailed distribution	745:767	a detailed distribution of NPs with cellular resolution in retinal tissue	745:817	Retinal whole mounts from rats injected in vivo with fluorescent PBCA NPs were processed for retina imaging ex vivo to obtain a detailed distribution of NPs with cellular resolution in retinal tissue.
31609624	1	57	theme	surface	316:322	arg1	charge					324:329	surface charge	316:329	surface charge	316:329	Minor changes in the composition of poloxamer 188-modified, DEAE-dextran-stabilized (PDD) polybutylcyanoacrylate (PBCA) nanoparticles (NPs), by altering the physicochemical parameters (such as size or surface charge), can substantially influence their delivery kinetics across the blood-retina barrier (BRB) in vivo.
31609624	1	58	theme	blood-retina	396:407	arg1	barrier					409:415	the blood-retina barrier	392:415	the blood-retina barrier (BRB) in vivo	392:429	Minor changes in the composition of poloxamer 188-modified, DEAE-dextran-stabilized (PDD) polybutylcyanoacrylate (PBCA) nanoparticles (NPs), by altering the physicochemical parameters (such as size or surface charge), can substantially influence their delivery kinetics across the blood-retina barrier (BRB) in vivo.
31609624	1	58	theme	blood-retina	396:407	arg1	BRB					418:420	BRB	418:420	BRB	418:420	Minor changes in the composition of poloxamer 188-modified, DEAE-dextran-stabilized (PDD) polybutylcyanoacrylate (PBCA) nanoparticles (NPs), by altering the physicochemical parameters (such as size or surface charge), can substantially influence their delivery kinetics across the blood-retina barrier (BRB) in vivo.
31609624	6	59	theme	central	1413:1419	arg1	CNS					1437:1439	CNS	1437:1439	CNS	1437:1439	Thus, systemic interactions significantly determine the potential of NPs to deliver markers or drugs to the central nervous system (CNS).
31609624	6	59	theme	central	1413:1419	arg1	system					1429:1434	the central nervous system	1409:1434	the central nervous system (CNS)	1409:1440	Thus, systemic interactions significantly determine the potential of NPs to deliver markers or drugs to the central nervous system (CNS).
31609624	3	60	dep	ex	727:728	arg1	vivo					730:733	vivo	730:733	vivo	730:733	Retinal whole mounts from rats injected in vivo with fluorescent PBCA NPs were processed for retina imaging ex vivo to obtain a detailed distribution of NPs with cellular resolution in retinal tissue.
31609624	4	61	theme	larger	878:883	arg1	size					885:888	a larger size	876:888	a larger size	876:888	In line with previous in vivo imaging results, NPs with a larger size and medium surface charge accumulated more readily in brain tissue, and they could be more easily detected in retinal ganglion cells (RGCs), demonstrating the potential of these NPs for drug delivery into neurons.
31609624	3	62	theme	fluorescent	672:682	arg1	NPs					689:691	fluorescent PBCA NPs	672:691	fluorescent PBCA NPs	672:691	Retinal whole mounts from rats injected in vivo with fluorescent PBCA NPs were processed for retina imaging ex vivo to obtain a detailed distribution of NPs with cellular resolution in retinal tissue.
31609624	3	63	theme	Retinal	619:625	arg1	mounts					633:638	Retinal whole mounts	619:638	Retinal whole mounts from rats injected in vivo with fluorescent PBCA NPs	619:691	Retinal whole mounts from rats injected in vivo with fluorescent PBCA NPs were processed for retina imaging ex vivo to obtain a detailed distribution of NPs with cellular resolution in retinal tissue.
31609624	5	64	theme	"	1292:1292	arg1	mechanism					1294:1302	a "steal effect" mechanism	1277:1302	a "steal effect" mechanism	1277:1302	The biodistribution of the NPs revealed a higher accumulation of small-sized NPs in peripheral organs, which may reduce the passage of these particles into brain tissue via a "steal effect" mechanism.
31609624	3	65	theme	whole	627:631	arg1	mounts					633:638	Retinal whole mounts	619:638	Retinal whole mounts from rats injected in vivo with fluorescent PBCA NPs	619:691	Retinal whole mounts from rats injected in vivo with fluorescent PBCA NPs were processed for retina imaging ex vivo to obtain a detailed distribution of NPs with cellular resolution in retinal tissue.
31609624	5	66	theme	small-sized	1169:1179	arg1	NPs					1181:1183	small-sized NPs	1169:1183	small-sized NPs	1169:1183	The biodistribution of the NPs revealed a higher accumulation of small-sized NPs in peripheral organs, which may reduce the passage of these particles into brain tissue via a "steal effect" mechanism.
31609624	4	67	theme	brain	944:948	arg1	tissue					950:955	brain tissue	944:955	brain tissue	944:955	In line with previous in vivo imaging results, NPs with a larger size and medium surface charge accumulated more readily in brain tissue, and they could be more easily detected in retinal ganglion cells (RGCs), demonstrating the potential of these NPs for drug delivery into neurons.
31609624	5	68	theme	peripheral	1188:1197	arg1	organs					1199:1204	peripheral organs	1188:1204	peripheral organs	1188:1204	The biodistribution of the NPs revealed a higher accumulation of small-sized NPs in peripheral organs, which may reduce the passage of these particles into brain tissue via a "steal effect" mechanism.
31609624	6	69	theme	systemic	1311:1318	arg1	interactions					1320:1331	systemic interactions	1311:1331	systemic interactions	1311:1331	Thus, systemic interactions significantly determine the potential of NPs to deliver markers or drugs to the central nervous system (CNS).
31609624	2	70	from	barriers	551:558	arg1	behaviors					510:518	these different behaviors	494:518	these different behaviors of NP variations at biological barriers	494:558	We now investigated the physicochemical mechanisms underlying these different behaviors of NP variations at biological barriers and their influence on the cellular and body distribution.
31609624	2	70	from	barriers	551:558	arg1	variations					526:535	NP variations	523:535	NP variations at biological barriers	523:558	We now investigated the physicochemical mechanisms underlying these different behaviors of NP variations at biological barriers and their influence on the cellular and body distribution.
31609624	5	71	theme	higher	1146:1151	arg1	accumulation					1153:1164	a higher accumulation	1144:1164	a higher accumulation of small-sized NPs in peripheral organs, which may reduce the passage of these particles into brain tissue via a "steal effect" mechanism	1144:1302	The biodistribution of the NPs revealed a higher accumulation of small-sized NPs in peripheral organs, which may reduce the passage of these particles into brain tissue via a "steal effect" mechanism.
31609624	1	72	dep	parameters	288:297	arg1	such					300:303	such	300:303	such	300:303	Minor changes in the composition of poloxamer 188-modified, DEAE-dextran-stabilized (PDD) polybutylcyanoacrylate (PBCA) nanoparticles (NPs), by altering the physicochemical parameters (such as size or surface charge), can substantially influence their delivery kinetics across the blood-retina barrier (BRB) in vivo.
31609624	3	73	theme	cellular	781:788	arg1	resolution					790:799	cellular resolution	781:799	cellular resolution	781:799	Retinal whole mounts from rats injected in vivo with fluorescent PBCA NPs were processed for retina imaging ex vivo to obtain a detailed distribution of NPs with cellular resolution in retinal tissue.
31609624	5	74	theme	effect	1286:1291	arg1	"					1292:1292	a "steal effect"	1277:1292	a "steal effect" mechanism	1277:1302	The biodistribution of the NPs revealed a higher accumulation of small-sized NPs in peripheral organs, which may reduce the passage of these particles into brain tissue via a "steal effect" mechanism.
31609624	4	75	dep	in	842:843	arg1	vivo					845:848	vivo	845:848	vivo	845:848	In line with previous in vivo imaging results, NPs with a larger size and medium surface charge accumulated more readily in brain tissue, and they could be more easily detected in retinal ganglion cells (RGCs), demonstrating the potential of these NPs for drug delivery into neurons.
31609624	4	76	theme	ganglion	1008:1015	arg1	cells					1017:1021	retinal ganglion cells	1000:1021	retinal ganglion cells (RGCs)	1000:1028	In line with previous in vivo imaging results, NPs with a larger size and medium surface charge accumulated more readily in brain tissue, and they could be more easily detected in retinal ganglion cells (RGCs), demonstrating the potential of these NPs for drug delivery into neurons.
31609624	4	76	theme	ganglion	1008:1015	arg1	RGCs					1024:1027	RGCs	1024:1027	RGCs	1024:1027	In line with previous in vivo imaging results, NPs with a larger size and medium surface charge accumulated more readily in brain tissue, and they could be more easily detected in retinal ganglion cells (RGCs), demonstrating the potential of these NPs for drug delivery into neurons.
31609624	3	77	theme	NPs	772:774	arg1	distribution					756:767	a detailed distribution	745:767	a detailed distribution of NPs with cellular resolution in retinal tissue	745:817	Retinal whole mounts from rats injected in vivo with fluorescent PBCA NPs were processed for retina imaging ex vivo to obtain a detailed distribution of NPs with cellular resolution in retinal tissue.
30180924	14	0	theme	RSCIC	1409:1413	arg1	MCT13T					1381:1386	MCT13T	1381:1386	MCT13T (=DSM 105457T=BAC RE RSCIC 7T)	1381:1417	The type strain is MCT13T (=DSM 105457T=BAC RE RSCIC 7T).
30180924	14	0	theme	RSCIC	1409:1413	arg1	7T					1415:1416	=DSM 105457T=BAC RE RSCIC 7T	1389:1416	=DSM 105457T=BAC RE RSCIC 7T	1389:1416	The type strain is MCT13T (=DSM 105457T=BAC RE RSCIC 7T).
30180924	11	1	theme	closest	993:999	arg1	relative					1001:1008	the closest relative	989:1008	the closest relative of MCT13T to be Sphingomonas koreensis (98.52 %)	989:1057	The 16S rRNA gene phylogenetic analysis performed on the whole sequence, showed the closest relative of MCT13T to be Sphingomonas koreensis (98.52 %); however, there are several genotypic and phenotypic differences between the novel isolate and the type strain JSS26T of S. koreensis.
30180924	12	2	theme	novel	1252:1256	arg1	species					1258:1264	a novel species	1250:1264	a novel species	1250:1264	On the basis of these results, strain MCT13T represents a novel species in the genus Sphingomonas, for which the name Sphingomonas turrisvirgatae sp.
30180924	2	3	theme	agar-pitting	113:124	arg1	colony					126:131	A yellow pigmented and agar-pitting colony	90:131	A yellow pigmented and agar-pitting colony	90:131	A yellow pigmented and agar-pitting colony was isolated from a water sample obtained from a drainage ditch within a disused system of constructed wetlands.
30180924	11	4	theme	16S	913:915	arg1	rRNA					917:920	16S rRNA	913:920	The 16S rRNA gene phylogenetic analysis	909:947	The 16S rRNA gene phylogenetic analysis performed on the whole sequence, showed the closest relative of MCT13T to be Sphingomonas koreensis (98.52 %); however, there are several genotypic and phenotypic differences between the novel isolate and the type strain JSS26T of S. koreensis.
30180924	4	5	theme	non-motile	384:393	arg1	strain					395:400	This rod-shaped, Gram-negative, oxidase- and catalase-positive, aerobic, non-spore-forming, and non-motile strain	288:400	This rod-shaped, Gram-negative, oxidase- and catalase-positive, aerobic, non-spore-forming, and non-motile strain	288:400	This rod-shaped, Gram-negative, oxidase- and catalase-positive, aerobic, non-spore-forming, and non-motile strain formed round colonies and grew optimally at pH 7.5±0.2, at 28-30 °C on LB agar, with 0-0.5 % NaCl.
30180924	12	6	theme	Sphingomonas	1312:1323	arg1	sp					1340:1341	the name Sphingomonas turrisvirgatae sp	1303:1341	the name Sphingomonas turrisvirgatae sp	1303:1341	On the basis of these results, strain MCT13T represents a novel species in the genus Sphingomonas, for which the name Sphingomonas turrisvirgatae sp.
30180924	2	7	theme	water	153:157	arg1	sample					159:164	a water sample	151:164	a water sample obtained from a drainage ditch within a disused system of constructed wetlands	151:243	A yellow pigmented and agar-pitting colony was isolated from a water sample obtained from a drainage ditch within a disused system of constructed wetlands.
30180924	4	8	theme	round	409:413	arg1	colonies					415:422	round colonies	409:422	round colonies	409:422	This rod-shaped, Gram-negative, oxidase- and catalase-positive, aerobic, non-spore-forming, and non-motile strain formed round colonies and grew optimally at pH 7.5±0.2, at 28-30 °C on LB agar, with 0-0.5 % NaCl.
30180924	12	9	theme	name	1307:1310	arg1	sp					1340:1341	the name Sphingomonas turrisvirgatae sp	1303:1341	the name Sphingomonas turrisvirgatae sp	1303:1341	On the basis of these results, strain MCT13T represents a novel species in the genus Sphingomonas, for which the name Sphingomonas turrisvirgatae sp.
30180924	9	10	theme	major	755:759	arg1	sphingoglycolipid					779:795	sphingoglycolipid	779:795	sphingoglycolipid	779:795	The major polar lipids were sphingoglycolipid, diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol.
30180924	9	10	theme	major	755:759	arg1	lipids					767:772	The major polar lipids	751:772	The major polar lipids	751:772	The major polar lipids were sphingoglycolipid, diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol.
30180924	5	11	theme	Sphingomonas	574:585	arg1	stricto					594:600	sensu stricto	588:600	sensu stricto	588:600	The 16S rRNA gene sequence analysis placed the MCT13T isolate within the Sphingomonas (sensu stricto) cluster.
30180924	5	11	theme	Sphingomonas	574:585	arg1	cluster					603:609	the Sphingomonas (sensu stricto) cluster	570:609	the Sphingomonas (sensu stricto) cluster	570:609	The 16S rRNA gene sequence analysis placed the MCT13T isolate within the Sphingomonas (sensu stricto) cluster.
30180924	10	12	theme	major	877:881	arg1	polyamine					883:891	The major polyamine	873:891	The major polyamine	873:891	The major polyamine was spermidine.
30180924	10	12	theme	major	877:881	arg1	spermidine					897:906	spermidine	897:906	spermidine	897:906	The major polyamine was spermidine.
30180924	14	13	theme	type	1366:1369	arg1	MCT13T					1381:1386	MCT13T	1381:1386	MCT13T (=DSM 105457T=BAC RE RSCIC 7T)	1381:1417	The type strain is MCT13T (=DSM 105457T=BAC RE RSCIC 7T).
30180924	14	13	theme	type	1366:1369	arg1	strain					1371:1376	The type strain	1362:1376	The type strain	1362:1376	The type strain is MCT13T (=DSM 105457T=BAC RE RSCIC 7T).
30180924	11	14	theme	Sphingomonas	1026:1037	arg1	koreensis					1039:1047	Sphingomonas koreensis	1026:1047	Sphingomonas koreensis (98.52 %)	1026:1057	The 16S rRNA gene phylogenetic analysis performed on the whole sequence, showed the closest relative of MCT13T to be Sphingomonas koreensis (98.52 %); however, there are several genotypic and phenotypic differences between the novel isolate and the type strain JSS26T of S. koreensis.
30180924	11	14	theme	Sphingomonas	1026:1037	arg1	%					1056:1056	98.52 %	1050:1056	98.52 %	1050:1056	The 16S rRNA gene phylogenetic analysis performed on the whole sequence, showed the closest relative of MCT13T to be Sphingomonas koreensis (98.52 %); however, there are several genotypic and phenotypic differences between the novel isolate and the type strain JSS26T of S. koreensis.
30180924	11	15	theme	phenotypic	1101:1110	arg1	differences					1112:1122	several genotypic and phenotypic differences	1079:1122	several genotypic and phenotypic differences between the novel isolate and the type strain JSS26T of S. koreensis	1079:1191	The 16S rRNA gene phylogenetic analysis performed on the whole sequence, showed the closest relative of MCT13T to be Sphingomonas koreensis (98.52 %); however, there are several genotypic and phenotypic differences between the novel isolate and the type strain JSS26T of S. koreensis.
30180924	4	16	theme	LB	473:474	arg1	agar					476:479	LB agar	473:479	LB agar	473:479	This rod-shaped, Gram-negative, oxidase- and catalase-positive, aerobic, non-spore-forming, and non-motile strain formed round colonies and grew optimally at pH 7.5±0.2, at 28-30 °C on LB agar, with 0-0.5 % NaCl.
30180924	5	17	theme	sensu	588:592	arg1	stricto					594:600	sensu stricto	588:600	sensu stricto	588:600	The 16S rRNA gene sequence analysis placed the MCT13T isolate within the Sphingomonas (sensu stricto) cluster.
30180924	5	17	theme	sensu	588:592	arg1	cluster					603:609	the Sphingomonas (sensu stricto) cluster	570:609	the Sphingomonas (sensu stricto) cluster	570:609	The 16S rRNA gene sequence analysis placed the MCT13T isolate within the Sphingomonas (sensu stricto) cluster.
30180924	4	18	theme	non-spore-forming	361:377	arg1	strain					395:400	This rod-shaped, Gram-negative, oxidase- and catalase-positive, aerobic, non-spore-forming, and non-motile strain	288:400	This rod-shaped, Gram-negative, oxidase- and catalase-positive, aerobic, non-spore-forming, and non-motile strain	288:400	This rod-shaped, Gram-negative, oxidase- and catalase-positive, aerobic, non-spore-forming, and non-motile strain formed round colonies and grew optimally at pH 7.5±0.2, at 28-30 °C on LB agar, with 0-0.5 % NaCl.
30180924	12	19	theme	turrisvirgatae	1325:1338	arg1	sp					1340:1341	the name Sphingomonas turrisvirgatae sp	1303:1341	the name Sphingomonas turrisvirgatae sp	1303:1341	On the basis of these results, strain MCT13T represents a novel species in the genus Sphingomonas, for which the name Sphingomonas turrisvirgatae sp.
30180924	4	20	theme	rod-shaped	293:302	arg1	strain					395:400	This rod-shaped, Gram-negative, oxidase- and catalase-positive, aerobic, non-spore-forming, and non-motile strain	288:400	This rod-shaped, Gram-negative, oxidase- and catalase-positive, aerobic, non-spore-forming, and non-motile strain	288:400	This rod-shaped, Gram-negative, oxidase- and catalase-positive, aerobic, non-spore-forming, and non-motile strain formed round colonies and grew optimally at pH 7.5±0.2, at 28-30 °C on LB agar, with 0-0.5 % NaCl.
30180924	0	21	theme	turrisvirgatae	13:26	arg1	sp					28:29	Sphingomonas turrisvirgatae sp	0:29	Sphingomonas turrisvirgatae sp.	0:30	Sphingomonas turrisvirgatae sp.
30180924	4	22	from	28-30 °C	461:468	arg1	agar					476:479	LB agar	473:479	LB agar	473:479	This rod-shaped, Gram-negative, oxidase- and catalase-positive, aerobic, non-spore-forming, and non-motile strain formed round colonies and grew optimally at pH 7.5±0.2, at 28-30 °C on LB agar, with 0-0.5 % NaCl.
30180924	0	23	theme	Sphingomonas	0:11	arg1	sp					28:29	Sphingomonas turrisvirgatae sp	0:29	Sphingomonas turrisvirgatae sp.	0:30	Sphingomonas turrisvirgatae sp.
30180924	11	24	theme	phylogenetic	927:938	arg1	analysis					940:947	The 16S rRNA gene phylogenetic analysis	909:947	The 16S rRNA gene phylogenetic analysis	909:947	The 16S rRNA gene phylogenetic analysis performed on the whole sequence, showed the closest relative of MCT13T to be Sphingomonas koreensis (98.52 %); however, there are several genotypic and phenotypic differences between the novel isolate and the type strain JSS26T of S. koreensis.
30180924	12	25	dep	results	1216:1222	arg1	the					1197:1199	the	1197:1199	the	1197:1199	On the basis of these results, strain MCT13T represents a novel species in the genus Sphingomonas, for which the name Sphingomonas turrisvirgatae sp.
30180924	12	25	dep	results	1216:1222	arg1	basis					1201:1205	basis	1201:1205	basis	1201:1205	On the basis of these results, strain MCT13T represents a novel species in the genus Sphingomonas, for which the name Sphingomonas turrisvirgatae sp.
30180924	12	26	theme	strain	1225:1230	arg1	MCT13T					1232:1237	strain MCT13T	1225:1237	strain MCT13T	1225:1237	On the basis of these results, strain MCT13T represents a novel species in the genus Sphingomonas, for which the name Sphingomonas turrisvirgatae sp.
30180924	5	27	theme	MCT13T	548:553	arg1	isolate					555:561	the MCT13T isolate	544:561	the MCT13T isolate	544:561	The 16S rRNA gene sequence analysis placed the MCT13T isolate within the Sphingomonas (sensu stricto) cluster.
30180924	11	28	theme	novel	1136:1140	arg1	isolate					1142:1148	the novel isolate	1132:1148	the novel isolate	1132:1148	The 16S rRNA gene phylogenetic analysis performed on the whole sequence, showed the closest relative of MCT13T to be Sphingomonas koreensis (98.52 %); however, there are several genotypic and phenotypic differences between the novel isolate and the type strain JSS26T of S. koreensis.
30180924	11	29	theme	rRNA	917:920	arg1	analysis					940:947	The 16S rRNA gene phylogenetic analysis	909:947	The 16S rRNA gene phylogenetic analysis	909:947	The 16S rRNA gene phylogenetic analysis performed on the whole sequence, showed the closest relative of MCT13T to be Sphingomonas koreensis (98.52 %); however, there are several genotypic and phenotypic differences between the novel isolate and the type strain JSS26T of S. koreensis.
30180924	5	30	theme	gene	514:517	arg1	analysis					528:535	The 16S rRNA gene sequence analysis	501:535	The 16S rRNA gene sequence analysis	501:535	The 16S rRNA gene sequence analysis placed the MCT13T isolate within the Sphingomonas (sensu stricto) cluster.
30180924	7	31	theme	only	648:651	arg1	Q10					677:679	Q10	677:679	Q10	677:679	The only observed ubiquinone was Q10.
30180924	7	31	theme	only	648:651	arg1	ubiquinone					662:671	The only observed ubiquinone	644:671	The only observed ubiquinone	644:671	The only observed ubiquinone was Q10.
30180924	11	32	theme	whole	966:970	arg1	sequence					972:979	the whole sequence	962:979	the whole sequence	962:979	The 16S rRNA gene phylogenetic analysis performed on the whole sequence, showed the closest relative of MCT13T to be Sphingomonas koreensis (98.52 %); however, there are several genotypic and phenotypic differences between the novel isolate and the type strain JSS26T of S. koreensis.
30180924	11	33	theme	gene	922:925	arg1	analysis					940:947	The 16S rRNA gene phylogenetic analysis	909:947	The 16S rRNA gene phylogenetic analysis	909:947	The 16S rRNA gene phylogenetic analysis performed on the whole sequence, showed the closest relative of MCT13T to be Sphingomonas koreensis (98.52 %); however, there are several genotypic and phenotypic differences between the novel isolate and the type strain JSS26T of S. koreensis.
30180924	11	34	theme	type	1158:1161	arg1	strain					1163:1168	the type strain	1154:1168	the type strain	1154:1168	The 16S rRNA gene phylogenetic analysis performed on the whole sequence, showed the closest relative of MCT13T to be Sphingomonas koreensis (98.52 %); however, there are several genotypic and phenotypic differences between the novel isolate and the type strain JSS26T of S. koreensis.
30180924	7	35	theme	observed	653:660	arg1	Q10					677:679	Q10	677:679	Q10	677:679	The only observed ubiquinone was Q10.
30180924	7	35	theme	observed	653:660	arg1	ubiquinone					662:671	The only observed ubiquinone	644:671	The only observed ubiquinone	644:671	The only observed ubiquinone was Q10.
30180924	2	36	theme	constructed	224:234	arg1	wetlands					236:243	constructed wetlands	224:243	constructed wetlands	224:243	A yellow pigmented and agar-pitting colony was isolated from a water sample obtained from a drainage ditch within a disused system of constructed wetlands.
30180924	14	37	theme	105457T=BAC	1394:1404	arg1	MCT13T					1381:1386	MCT13T	1381:1386	MCT13T (=DSM 105457T=BAC RE RSCIC 7T)	1381:1417	The type strain is MCT13T (=DSM 105457T=BAC RE RSCIC 7T).
30180924	14	37	theme	105457T=BAC	1394:1404	arg1	7T					1415:1416	=DSM 105457T=BAC RE RSCIC 7T	1389:1416	=DSM 105457T=BAC RE RSCIC 7T	1389:1416	The type strain is MCT13T (=DSM 105457T=BAC RE RSCIC 7T).
30180924	2	38	theme	pigmented	99:107	arg1	colony					126:131	A yellow pigmented and agar-pitting colony	90:131	A yellow pigmented and agar-pitting colony	90:131	A yellow pigmented and agar-pitting colony was isolated from a water sample obtained from a drainage ditch within a disused system of constructed wetlands.
30180924	4	39	theme	0-0.5 	487:492	arg1	%					493:493	%	493:493	%	493:493	This rod-shaped, Gram-negative, oxidase- and catalase-positive, aerobic, non-spore-forming, and non-motile strain formed round colonies and grew optimally at pH 7.5±0.2, at 28-30 °C on LB agar, with 0-0.5 % NaCl.
30180924	8	40	theme	fatty	692:696	arg1	acids					698:702	The major fatty acids	682:702	The major fatty acids	682:702	The major fatty acids included C17 : 1ω6c and C18 : 1ω7c/C18 : 1ω6c.
30180924	4	41	theme	%	493:493	arg1	 NaCl					494:498	0-0.5 % NaCl	487:498	0-0.5 % NaCl	487:498	This rod-shaped, Gram-negative, oxidase- and catalase-positive, aerobic, non-spore-forming, and non-motile strain formed round colonies and grew optimally at pH 7.5±0.2, at 28-30 °C on LB agar, with 0-0.5 % NaCl.
30180924	8	42	dep	C17 	713:716	arg1	C18 					728:731	C18 	728:731	C18 	728:731	The major fatty acids included C17 : 1ω6c and C18 : 1ω7c/C18 : 1ω6c.
30180924	8	42	dep	C17 	713:716	arg1	 1ω6c					718:722	 1ω6c	718:722	 1ω6c	718:722	The major fatty acids included C17 : 1ω6c and C18 : 1ω7c/C18 : 1ω6c.
30180924	8	42	dep	C17 	713:716	arg1	 1ω6c					744:748	 1ω6c	744:748	 1ω6c	744:748	The major fatty acids included C17 : 1ω6c and C18 : 1ω7c/C18 : 1ω6c.
30180924	8	42	dep	C17 	713:716	arg1	 1ω7c/C18 					733:742	 1ω7c/C18 	733:742	 1ω7c/C18 	733:742	The major fatty acids included C17 : 1ω6c and C18 : 1ω7c/C18 : 1ω6c.
30180924	5	43	theme	rRNA	509:512	arg1	analysis					528:535	The 16S rRNA gene sequence analysis	501:535	The 16S rRNA gene sequence analysis	501:535	The 16S rRNA gene sequence analysis placed the MCT13T isolate within the Sphingomonas (sensu stricto) cluster.
30180924	8	44	theme	major	686:690	arg1	acids					698:702	The major fatty acids	682:702	The major fatty acids	682:702	The major fatty acids included C17 : 1ω6c and C18 : 1ω7c/C18 : 1ω6c.
30180924	2	45	theme	disused	206:212	arg1	system					214:219	a disused system	204:219	a disused system of constructed wetlands	204:243	A yellow pigmented and agar-pitting colony was isolated from a water sample obtained from a drainage ditch within a disused system of constructed wetlands.
30180924	4	46	theme	aerobic	352:358	arg1	strain					395:400	This rod-shaped, Gram-negative, oxidase- and catalase-positive, aerobic, non-spore-forming, and non-motile strain	288:400	This rod-shaped, Gram-negative, oxidase- and catalase-positive, aerobic, non-spore-forming, and non-motile strain	288:400	This rod-shaped, Gram-negative, oxidase- and catalase-positive, aerobic, non-spore-forming, and non-motile strain formed round colonies and grew optimally at pH 7.5±0.2, at 28-30 °C on LB agar, with 0-0.5 % NaCl.
30180924	6	47	theme	DNA	616:618	arg1	G+C content					620:630	The DNA G+C content	612:630	The DNA G+C content	612:630	The DNA G+C content was 65.3 %.
30180924	6	47	theme	DNA	616:618	arg1	%					641:641	65.3 %	636:641	65.3 %	636:641	The DNA G+C content was 65.3 %.
30180924	1	48	theme	agar-degrading	41:54	arg1	species					56:62	an agar-degrading species	38:62	an agar-degrading species	38:62	nov., an agar-degrading species isolated from freshwater.
30180924	11	49	theme	genotypic	1087:1095	arg1	differences					1112:1122	several genotypic and phenotypic differences	1079:1122	several genotypic and phenotypic differences between the novel isolate and the type strain JSS26T of S. koreensis	1079:1191	The 16S rRNA gene phylogenetic analysis performed on the whole sequence, showed the closest relative of MCT13T to be Sphingomonas koreensis (98.52 %); however, there are several genotypic and phenotypic differences between the novel isolate and the type strain JSS26T of S. koreensis.
30180924	5	50	theme	sequence	519:526	arg1	analysis					528:535	The 16S rRNA gene sequence analysis	501:535	The 16S rRNA gene sequence analysis	501:535	The 16S rRNA gene sequence analysis placed the MCT13T isolate within the Sphingomonas (sensu stricto) cluster.
30180924	2	51	attach	isolated	137:144	arg1	sample					159:164	a water sample	151:164	a water sample obtained from a drainage ditch within a disused system of constructed wetlands	151:243	A yellow pigmented and agar-pitting colony was isolated from a water sample obtained from a drainage ditch within a disused system of constructed wetlands.
30180924	2	51	attach	isolated	137:144	arg2	colony					126:131	A yellow pigmented and agar-pitting colony	90:131	A yellow pigmented and agar-pitting colony	90:131	A yellow pigmented and agar-pitting colony was isolated from a water sample obtained from a drainage ditch within a disused system of constructed wetlands.
30180924	9	52	theme	polar	761:765	arg1	sphingoglycolipid					779:795	sphingoglycolipid	779:795	sphingoglycolipid	779:795	The major polar lipids were sphingoglycolipid, diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol.
30180924	9	52	theme	polar	761:765	arg1	lipids					767:772	The major polar lipids	751:772	The major polar lipids	751:772	The major polar lipids were sphingoglycolipid, diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol.
30180924	11	53	theme	koreensis	1183:1191	arg1	isolate					1142:1148	the novel isolate	1132:1148	the novel isolate	1132:1148	The 16S rRNA gene phylogenetic analysis performed on the whole sequence, showed the closest relative of MCT13T to be Sphingomonas koreensis (98.52 %); however, there are several genotypic and phenotypic differences between the novel isolate and the type strain JSS26T of S. koreensis.
30180924	11	53	theme	koreensis	1183:1191	arg1	strain					1163:1168	the type strain	1154:1168	the type strain	1154:1168	The 16S rRNA gene phylogenetic analysis performed on the whole sequence, showed the closest relative of MCT13T to be Sphingomonas koreensis (98.52 %); however, there are several genotypic and phenotypic differences between the novel isolate and the type strain JSS26T of S. koreensis.
30180924	2	54	theme	ditch	191:195	arg1	drainage					182:189	a drainage	180:189	a drainage ditch within a disused system of constructed wetlands	180:243	A yellow pigmented and agar-pitting colony was isolated from a water sample obtained from a drainage ditch within a disused system of constructed wetlands.
30180924	5	55	theme	16S	505:507	arg1	rRNA					509:512	The 16S rRNA	501:512	The 16S rRNA gene sequence analysis	501:535	The 16S rRNA gene sequence analysis placed the MCT13T isolate within the Sphingomonas (sensu stricto) cluster.
30180924	4	56	theme	Gram-negative	305:317	arg1	strain					395:400	This rod-shaped, Gram-negative, oxidase- and catalase-positive, aerobic, non-spore-forming, and non-motile strain	288:400	This rod-shaped, Gram-negative, oxidase- and catalase-positive, aerobic, non-spore-forming, and non-motile strain	288:400	This rod-shaped, Gram-negative, oxidase- and catalase-positive, aerobic, non-spore-forming, and non-motile strain formed round colonies and grew optimally at pH 7.5±0.2, at 28-30 °C on LB agar, with 0-0.5 % NaCl.
30180924	11	57	dep	isolate	1142:1148	arg1	JSS26T					1170:1175	JSS26T	1170:1175	JSS26T	1170:1175	The 16S rRNA gene phylogenetic analysis performed on the whole sequence, showed the closest relative of MCT13T to be Sphingomonas koreensis (98.52 %); however, there are several genotypic and phenotypic differences between the novel isolate and the type strain JSS26T of S. koreensis.
30180924	14	58	theme	RE	1406:1407	arg1	MCT13T					1381:1386	MCT13T	1381:1386	MCT13T (=DSM 105457T=BAC RE RSCIC 7T)	1381:1417	The type strain is MCT13T (=DSM 105457T=BAC RE RSCIC 7T).
30180924	14	58	theme	RE	1406:1407	arg1	7T					1415:1416	=DSM 105457T=BAC RE RSCIC 7T	1389:1416	=DSM 105457T=BAC RE RSCIC 7T	1389:1416	The type strain is MCT13T (=DSM 105457T=BAC RE RSCIC 7T).
30180924	12	59	theme	genus	1273:1277	arg1	Sphingomonas					1279:1290	the genus Sphingomonas	1269:1290	the genus Sphingomonas	1269:1290	On the basis of these results, strain MCT13T represents a novel species in the genus Sphingomonas, for which the name Sphingomonas turrisvirgatae sp.
30180924	14	60	theme	=DSM	1389:1392	arg1	MCT13T					1381:1386	MCT13T	1381:1386	MCT13T (=DSM 105457T=BAC RE RSCIC 7T)	1381:1417	The type strain is MCT13T (=DSM 105457T=BAC RE RSCIC 7T).
30180924	14	60	theme	=DSM	1389:1392	arg1	7T					1415:1416	=DSM 105457T=BAC RE RSCIC 7T	1389:1416	=DSM 105457T=BAC RE RSCIC 7T	1389:1416	The type strain is MCT13T (=DSM 105457T=BAC RE RSCIC 7T).
30180924	11	61	theme	several	1079:1085	arg1	differences					1112:1122	several genotypic and phenotypic differences	1079:1122	several genotypic and phenotypic differences between the novel isolate and the type strain JSS26T of S. koreensis	1079:1191	The 16S rRNA gene phylogenetic analysis performed on the whole sequence, showed the closest relative of MCT13T to be Sphingomonas koreensis (98.52 %); however, there are several genotypic and phenotypic differences between the novel isolate and the type strain JSS26T of S. koreensis.
30180924	2	62	theme	wetlands	236:243	arg1	system					214:219	a disused system	204:219	a disused system of constructed wetlands	204:243	A yellow pigmented and agar-pitting colony was isolated from a water sample obtained from a drainage ditch within a disused system of constructed wetlands.
30180924	4	63	theme	oxidase-	320:327	arg1	strain					395:400	This rod-shaped, Gram-negative, oxidase- and catalase-positive, aerobic, non-spore-forming, and non-motile strain	288:400	This rod-shaped, Gram-negative, oxidase- and catalase-positive, aerobic, non-spore-forming, and non-motile strain	288:400	This rod-shaped, Gram-negative, oxidase- and catalase-positive, aerobic, non-spore-forming, and non-motile strain formed round colonies and grew optimally at pH 7.5±0.2, at 28-30 °C on LB agar, with 0-0.5 % NaCl.
30180924	11	64	theme	MCT13T	1013:1018	arg1	relative					1001:1008	the closest relative	989:1008	the closest relative of MCT13T to be Sphingomonas koreensis (98.52 %)	989:1057	The 16S rRNA gene phylogenetic analysis performed on the whole sequence, showed the closest relative of MCT13T to be Sphingomonas koreensis (98.52 %); however, there are several genotypic and phenotypic differences between the novel isolate and the type strain JSS26T of S. koreensis.
30180924	12	65	from	species	1258:1264	arg1	Sphingomonas					1279:1290	the genus Sphingomonas	1269:1290	the genus Sphingomonas	1269:1290	On the basis of these results, strain MCT13T represents a novel species in the genus Sphingomonas, for which the name Sphingomonas turrisvirgatae sp.
30180924	2	66	theme	yellow	92:97	arg1	colony					126:131	A yellow pigmented and agar-pitting colony	90:131	A yellow pigmented and agar-pitting colony	90:131	A yellow pigmented and agar-pitting colony was isolated from a water sample obtained from a drainage ditch within a disused system of constructed wetlands.
30729887	0	0	theme	diabetes	95:102	arg1	treatment					104:112	diabetes treatment	95:112	diabetes treatment	95:112	Stability and biological testing of taurine-conjugated bile acid antioxidant microcapsules for diabetes treatment.
30729887	1	1	contain	possess	149:155	arg2	effects					192:198	positive formulation-stabilization effects	157:198	positive formulation-stabilization effects	157:198	AIM Taurine-conjugated bile acids possess positive formulation-stabilization effects, which are desirable in diabetes treatments.
30729887	1	1	contain	possess	149:155	arg1	acids					143:147	AIM Taurine-conjugated bile acids	115:147	AIM Taurine-conjugated bile acids	115:147	AIM Taurine-conjugated bile acids possess positive formulation-stabilization effects, which are desirable in diabetes treatments.
30729887	1	2	from	desirable	211:219	arg1	treatments					233:242	diabetes treatments	224:242	diabetes treatments	224:242	AIM Taurine-conjugated bile acids possess positive formulation-stabilization effects, which are desirable in diabetes treatments.
30729887	3	3	theme	PB-TCA	498:503	arg1	formulations					505:516	new PB-TCA formulations	494:516	new PB-TCA formulations using new polymers	494:535	This study aimed to design new PB-TCA formulations using new polymers, and examine antioxidant and antidiabetic effects using β-cells for PB with or without TCA.
30729887	2	4	theme	drug	390:393	arg1	delivery					362:369	the delivery	358:369	the delivery of the antioxidant drug, probucol (PB)	358:408	The taurine-conjugated bile acid, taurocholic acid (TCA), has shown promising formulation-stabilizing effects on the delivery of the antioxidant drug, probucol (PB), but success is limited due to its poor release profile.
30729887	2	5	theme	promising	313:321	arg1	effects					347:353	promising formulation-stabilizing effects	313:353	promising formulation-stabilizing effects on the delivery of the antioxidant drug, probucol (PB)	313:408	The taurine-conjugated bile acid, taurocholic acid (TCA), has shown promising formulation-stabilizing effects on the delivery of the antioxidant drug, probucol (PB), but success is limited due to its poor release profile.
30729887	1	6	theme	Taurine-conjugated	119:136	arg1	acids					143:147	AIM Taurine-conjugated bile acids	115:147	AIM Taurine-conjugated bile acids	115:147	AIM Taurine-conjugated bile acids possess positive formulation-stabilization effects, which are desirable in diabetes treatments.
30729887	6	7	theme	oral	1047:1050	arg1	delivery					1052:1059	the oral delivery	1043:1059	the oral delivery of anti-diabetic compounds	1043:1086	CONCLUSION TCA can optimize the oral delivery of anti-diabetic compounds.
30729887	4	8	theme	polymers	728:735	arg1	esters					699:704	alginate-insoluble esters	680:704	alginate-insoluble esters of polymethylacrylate polymers encapsulating PB and TCA	680:760	MATERIALS AND METHODS Different formulations using alginate-insoluble esters of polymethylacrylate polymers encapsulating PB and TCA were developed, microencapsulated and examined for stability and biological activity.
30729887	5	9	theme	polymethylacrylate	965:982	arg1	composition					984:994	polymethylacrylate composition	965:994	polymethylacrylate composition	965:994	RESULTS TCA addition to new PB matrices improved osmotic and mechanical properties, and this effect was dependent on polymethylacrylate composition and concentration.
30729887	4	10	theme	polymethylacrylate	709:726	arg1	polymers					728:735	polymethylacrylate polymers	709:735	polymethylacrylate polymers encapsulating PB and TCA	709:760	MATERIALS AND METHODS Different formulations using alginate-insoluble esters of polymethylacrylate polymers encapsulating PB and TCA were developed, microencapsulated and examined for stability and biological activity.
30729887	2	11	theme	antioxidant	378:388	arg1	probucol					396:403	probucol	396:403	probucol (PB)	396:408	The taurine-conjugated bile acid, taurocholic acid (TCA), has shown promising formulation-stabilizing effects on the delivery of the antioxidant drug, probucol (PB), but success is limited due to its poor release profile.
30729887	2	11	theme	antioxidant	378:388	arg1	drug					390:393	the antioxidant drug	374:393	the antioxidant drug	374:393	The taurine-conjugated bile acid, taurocholic acid (TCA), has shown promising formulation-stabilizing effects on the delivery of the antioxidant drug, probucol (PB), but success is limited due to its poor release profile.
30729887	5	12	theme	RESULTS	848:854	arg1	addition					860:867	RESULTS TCA addition	848:867	RESULTS TCA addition to new PB matrices	848:886	RESULTS TCA addition to new PB matrices improved osmotic and mechanical properties, and this effect was dependent on polymethylacrylate composition and concentration.
30729887	5	13	theme	mechanical	909:918	arg1	properties					920:929	osmotic and mechanical properties	897:929	osmotic and mechanical properties	897:929	RESULTS TCA addition to new PB matrices improved osmotic and mechanical properties, and this effect was dependent on polymethylacrylate composition and concentration.
30729887	5	14	theme	osmotic	897:903	arg1	properties					920:929	osmotic and mechanical properties	897:929	osmotic and mechanical properties	897:929	RESULTS TCA addition to new PB matrices improved osmotic and mechanical properties, and this effect was dependent on polymethylacrylate composition and concentration.
30729887	5	15	theme	TCA	856:858	arg1	addition					860:867	RESULTS TCA addition	848:867	RESULTS TCA addition to new PB matrices	848:886	RESULTS TCA addition to new PB matrices improved osmotic and mechanical properties, and this effect was dependent on polymethylacrylate composition and concentration.
30729887	4	16	theme	Different	651:659	arg1	formulations					661:672	Different formulations	651:672	Different formulations	651:672	MATERIALS AND METHODS Different formulations using alginate-insoluble esters of polymethylacrylate polymers encapsulating PB and TCA were developed, microencapsulated and examined for stability and biological activity.
30729887	2	17	theme	release	450:456	arg1	profile					458:464	its poor release profile	441:464	its poor release profile	441:464	The taurine-conjugated bile acid, taurocholic acid (TCA), has shown promising formulation-stabilizing effects on the delivery of the antioxidant drug, probucol (PB), but success is limited due to its poor release profile.
30729887	3	18	theme	antidiabetic	566:577	arg1	effects					579:585	antioxidant and antidiabetic effects	550:585	antioxidant and antidiabetic effects using β-cells for PB with or without TCA	550:626	This study aimed to design new PB-TCA formulations using new polymers, and examine antioxidant and antidiabetic effects using β-cells for PB with or without TCA.
30729887	4	19	theme	alginate-insoluble	680:697	arg1	esters					699:704	alginate-insoluble esters	680:704	alginate-insoluble esters of polymethylacrylate polymers encapsulating PB and TCA	680:760	MATERIALS AND METHODS Different formulations using alginate-insoluble esters of polymethylacrylate polymers encapsulating PB and TCA were developed, microencapsulated and examined for stability and biological activity.
30729887	2	20	theme	poor	445:448	arg1	profile					458:464	its poor release profile	441:464	its poor release profile	441:464	The taurine-conjugated bile acid, taurocholic acid (TCA), has shown promising formulation-stabilizing effects on the delivery of the antioxidant drug, probucol (PB), but success is limited due to its poor release profile.
30729887	1	21	theme	diabetes	224:231	arg1	treatments					233:242	diabetes treatments	224:242	diabetes treatments	224:242	AIM Taurine-conjugated bile acids possess positive formulation-stabilization effects, which are desirable in diabetes treatments.
30729887	3	22	theme	antioxidant	550:560	arg1	effects					579:585	antioxidant and antidiabetic effects	550:585	antioxidant and antidiabetic effects using β-cells for PB with or without TCA	550:626	This study aimed to design new PB-TCA formulations using new polymers, and examine antioxidant and antidiabetic effects using β-cells for PB with or without TCA.
30729887	5	23	theme	new	872:874	arg1	matrices					879:886	new PB matrices	872:886	new PB matrices	872:886	RESULTS TCA addition to new PB matrices improved osmotic and mechanical properties, and this effect was dependent on polymethylacrylate composition and concentration.
30729887	0	24	theme	biological	14:23	arg1	testing					25:31	biological testing	14:31	biological testing	14:31	Stability and biological testing of taurine-conjugated bile acid antioxidant microcapsules for diabetes treatment.
30729887	2	25	theme	formulation-stabilizing	323:345	arg1	effects					347:353	promising formulation-stabilizing effects	313:353	promising formulation-stabilizing effects on the delivery of the antioxidant drug, probucol (PB)	313:408	The taurine-conjugated bile acid, taurocholic acid (TCA), has shown promising formulation-stabilizing effects on the delivery of the antioxidant drug, probucol (PB), but success is limited due to its poor release profile.
30729887	5	26	theme	PB	876:877	arg1	matrices					879:886	new PB matrices	872:886	new PB matrices	872:886	RESULTS TCA addition to new PB matrices improved osmotic and mechanical properties, and this effect was dependent on polymethylacrylate composition and concentration.
30729887	0	27	theme	taurine-conjugated	36:53	arg1	antioxidant					65:75	taurine-conjugated bile acid antioxidant	36:75	taurine-conjugated bile acid antioxidant	36:75	Stability and biological testing of taurine-conjugated bile acid antioxidant microcapsules for diabetes treatment.
30729887	6	28	theme	CONCLUSION	1015:1024	arg1	TCA					1026:1028	CONCLUSION TCA	1015:1028	CONCLUSION TCA	1015:1028	CONCLUSION TCA can optimize the oral delivery of anti-diabetic compounds.
30729887	2	29	theme	taurocholic	279:289	arg1	TCA					297:299	TCA	297:299	TCA	297:299	The taurine-conjugated bile acid, taurocholic acid (TCA), has shown promising formulation-stabilizing effects on the delivery of the antioxidant drug, probucol (PB), but success is limited due to its poor release profile.
30729887	2	29	theme	taurocholic	279:289	arg1	acid					273:276	The taurine-conjugated bile acid	245:276	The taurine-conjugated bile acid	245:276	The taurine-conjugated bile acid, taurocholic acid (TCA), has shown promising formulation-stabilizing effects on the delivery of the antioxidant drug, probucol (PB), but success is limited due to its poor release profile.
30729887	2	29	theme	taurocholic	279:289	arg1	acid					291:294	taurocholic acid	279:294	taurocholic acid (TCA)	279:300	The taurine-conjugated bile acid, taurocholic acid (TCA), has shown promising formulation-stabilizing effects on the delivery of the antioxidant drug, probucol (PB), but success is limited due to its poor release profile.
30729887	0	30	theme	acid	60:63	arg1	antioxidant					65:75	taurine-conjugated bile acid antioxidant	36:75	taurine-conjugated bile acid antioxidant	36:75	Stability and biological testing of taurine-conjugated bile acid antioxidant microcapsules for diabetes treatment.
30729887	4	31	dep	MATERIALS	629:637	arg1	formulations					661:672	Different formulations	651:672	Different formulations	651:672	MATERIALS AND METHODS Different formulations using alginate-insoluble esters of polymethylacrylate polymers encapsulating PB and TCA were developed, microencapsulated and examined for stability and biological activity.
30729887	1	32	theme	bile	138:141	arg1	acids					143:147	AIM Taurine-conjugated bile acids	115:147	AIM Taurine-conjugated bile acids	115:147	AIM Taurine-conjugated bile acids possess positive formulation-stabilization effects, which are desirable in diabetes treatments.
30729887	0	33	theme	bile	55:58	arg1	antioxidant					65:75	taurine-conjugated bile acid antioxidant	36:75	taurine-conjugated bile acid antioxidant	36:75	Stability and biological testing of taurine-conjugated bile acid antioxidant microcapsules for diabetes treatment.
30729887	2	34	theme	taurine-conjugated	249:266	arg1	acid					273:276	The taurine-conjugated bile acid	245:276	The taurine-conjugated bile acid	245:276	The taurine-conjugated bile acid, taurocholic acid (TCA), has shown promising formulation-stabilizing effects on the delivery of the antioxidant drug, probucol (PB), but success is limited due to its poor release profile.
30729887	2	34	theme	taurine-conjugated	249:266	arg1	acid					291:294	taurocholic acid	279:294	taurocholic acid (TCA)	279:300	The taurine-conjugated bile acid, taurocholic acid (TCA), has shown promising formulation-stabilizing effects on the delivery of the antioxidant drug, probucol (PB), but success is limited due to its poor release profile.
30729887	2	35	theme	bile	268:271	arg1	acid					273:276	The taurine-conjugated bile acid	245:276	The taurine-conjugated bile acid	245:276	The taurine-conjugated bile acid, taurocholic acid (TCA), has shown promising formulation-stabilizing effects on the delivery of the antioxidant drug, probucol (PB), but success is limited due to its poor release profile.
30729887	2	35	theme	bile	268:271	arg1	acid					291:294	taurocholic acid	279:294	taurocholic acid (TCA)	279:300	The taurine-conjugated bile acid, taurocholic acid (TCA), has shown promising formulation-stabilizing effects on the delivery of the antioxidant drug, probucol (PB), but success is limited due to its poor release profile.
30729887	1	36	from	treatments	233:242	arg1	desirable					211:219	desirable	211:219	desirable	211:219	AIM Taurine-conjugated bile acids possess positive formulation-stabilization effects, which are desirable in diabetes treatments.
30729887	3	37	theme	new	494:496	arg1	formulations					505:516	new PB-TCA formulations	494:516	new PB-TCA formulations using new polymers	494:535	This study aimed to design new PB-TCA formulations using new polymers, and examine antioxidant and antidiabetic effects using β-cells for PB with or without TCA.
30729887	4	38	theme	biological	827:836	arg1	activity					838:845	biological activity	827:845	biological activity	827:845	MATERIALS AND METHODS Different formulations using alginate-insoluble esters of polymethylacrylate polymers encapsulating PB and TCA were developed, microencapsulated and examined for stability and biological activity.
30729887	1	39	theme	positive	157:164	arg1	effects					192:198	positive formulation-stabilization effects	157:198	positive formulation-stabilization effects	157:198	AIM Taurine-conjugated bile acids possess positive formulation-stabilization effects, which are desirable in diabetes treatments.
30729887	1	40	theme	formulation-stabilization	166:190	arg1	effects					192:198	positive formulation-stabilization effects	157:198	positive formulation-stabilization effects	157:198	AIM Taurine-conjugated bile acids possess positive formulation-stabilization effects, which are desirable in diabetes treatments.
30729887	6	41	theme	compounds	1078:1086	arg1	delivery					1052:1059	the oral delivery	1043:1059	the oral delivery of anti-diabetic compounds	1043:1086	CONCLUSION TCA can optimize the oral delivery of anti-diabetic compounds.
30729887	0	42	theme	antioxidant	65:75	arg1	Stability					0:8	Stability	0:8	Stability	0:8	Stability and biological testing of taurine-conjugated bile acid antioxidant microcapsules for diabetes treatment.
30729887	0	42	theme	antioxidant	65:75	arg1	testing					25:31	biological testing	14:31	biological testing	14:31	Stability and biological testing of taurine-conjugated bile acid antioxidant microcapsules for diabetes treatment.
30729887	3	43	theme	new	524:526	arg1	polymers					528:535	new polymers	524:535	new polymers	524:535	This study aimed to design new PB-TCA formulations using new polymers, and examine antioxidant and antidiabetic effects using β-cells for PB with or without TCA.
30729887	2	44	from	effects	347:353	arg1	delivery					362:369	the delivery	358:369	the delivery of the antioxidant drug, probucol (PB)	358:408	The taurine-conjugated bile acid, taurocholic acid (TCA), has shown promising formulation-stabilizing effects on the delivery of the antioxidant drug, probucol (PB), but success is limited due to its poor release profile.
30729887	6	45	theme	anti-diabetic	1064:1076	arg1	compounds					1078:1086	anti-diabetic compounds	1064:1086	anti-diabetic compounds	1064:1086	CONCLUSION TCA can optimize the oral delivery of anti-diabetic compounds.
29729694	0	0	theme	endoglucanase	79:91	arg1	production					65:74	enhanced production	56:74	enhanced production of endoglucanase by locally isolated fungal strain in solid state fermentation	56:153	Influence of medium composition and physical factors on enhanced production of endoglucanase by locally isolated fungal strain in solid state fermentation.
29729694	5	1	theme	hydrolysis	752:761	arg1	zone					763:766	cellulose hydrolysis zone	742:766	cellulose hydrolysis zone	742:766	Qualitative screening was carried out on the basis of cellulose hydrolysis zone.
29729694	9	2	theme	size	1152:1155	arg1	1.5ml					1157:1161	inoculum size 1.5ml	1143:1161	inoculum size 1.5ml	1143:1161	The maximal enzyme productivity was recorded in 72 hours, 40°C, pH 5, inoculum size 1.5ml, and moisture content (1:1).
29729694	9	2	theme	size	1152:1155	arg1	hours					1124:1128	72 hours	1121:1128	72 hours	1121:1128	The maximal enzyme productivity was recorded in 72 hours, 40°C, pH 5, inoculum size 1.5ml, and moisture content (1:1).
29729694	5	3	theme	zone	763:766	arg1	basis					733:737	the basis	729:737	the basis of cellulose hydrolysis zone	729:766	Qualitative screening was carried out on the basis of cellulose hydrolysis zone.
29729694	8	4	theme	wheat	1034:1038	arg1	bran					1040:1043	M 2 containing wheat bran	1019:1043	M 2 containing wheat bran	1019:1043	Different fermentation media were evaluated and M 2 containing wheat bran gave maximum EG production.
29729694	0	5	from	Influence	0:8	arg1	production					65:74	enhanced production	56:74	enhanced production of endoglucanase by locally isolated fungal strain in solid state fermentation	56:153	Influence of medium composition and physical factors on enhanced production of endoglucanase by locally isolated fungal strain in solid state fermentation.
29729694	8	6	theme	maximum	1050:1056	arg1	production					1061:1070	maximum EG production	1050:1070	maximum EG production	1050:1070	Different fermentation media were evaluated and M 2 containing wheat bran gave maximum EG production.
29729694	7	7	dep	selected	901:908	arg1	identified					910:919	identified	910:919	was selected identified	897:919	The fungal culture, showing highest EG potential was selected identified and assigned the code Aspergillus fumigatus BBT2.
29729694	2	8	theme	cellulose	289:297	arg1	hydrolysis					275:284	the catalytic hydrolysis	261:284	the catalytic hydrolysis of cellulose	261:297	Endoglucanase are involved in the catalytic hydrolysis of cellulose and plays a pivotal role in different sectors like pharmaceutical, textile, detergent, and food processing as well as paper and pulp industry.
29729694	7	9	theme	highest	876:882	arg1	potential					887:895	highest EG potential	876:895	highest EG potential	876:895	The fungal culture, showing highest EG potential was selected identified and assigned the code Aspergillus fumigatus BBT2.
29729694	7	10	dep	Aspergillus	943:953	arg1	fumigatus					955:963	fumigatus	955:963	fumigatus	955:963	The fungal culture, showing highest EG potential was selected identified and assigned the code Aspergillus fumigatus BBT2.
29729694	3	11	theme	better	539:544	arg1	option					546:551	a better option	537:551	a better option over other chemical catalysts	537:581	With consumers getting more and more aware of environmental issues, industries find enzymes as a better option over other chemical catalysts.
29729694	9	12	theme	maximal	1077:1083	arg1	productivity					1092:1103	The maximal enzyme productivity	1073:1103	The maximal enzyme productivity	1073:1103	The maximal enzyme productivity was recorded in 72 hours, 40°C, pH 5, inoculum size 1.5ml, and moisture content (1:1).
29729694	0	13	theme	fungal	113:118	arg1	strain					120:125	locally isolated fungal strain	96:125	locally isolated fungal strain	96:125	Influence of medium composition and physical factors on enhanced production of endoglucanase by locally isolated fungal strain in solid state fermentation.
29729694	9	14	theme	moisture	1168:1175	arg1	1:1					1186:1188	1:1	1186:1188	1:1	1186:1188	The maximal enzyme productivity was recorded in 72 hours, 40°C, pH 5, inoculum size 1.5ml, and moisture content (1:1).
29729694	9	14	theme	moisture	1168:1175	arg1	content					1177:1183	moisture content	1168:1183	moisture content (1:1)	1168:1189	The maximal enzyme productivity was recorded in 72 hours, 40°C, pH 5, inoculum size 1.5ml, and moisture content (1:1).
29729694	9	14	theme	moisture	1168:1175	arg1	hours					1124:1128	72 hours	1121:1128	72 hours	1121:1128	The maximal enzyme productivity was recorded in 72 hours, 40°C, pH 5, inoculum size 1.5ml, and moisture content (1:1).
29729694	9	15	theme	enzyme	1085:1090	arg1	productivity					1092:1103	The maximal enzyme productivity	1073:1103	The maximal enzyme productivity	1073:1103	The maximal enzyme productivity was recorded in 72 hours, 40°C, pH 5, inoculum size 1.5ml, and moisture content (1:1).
29729694	0	16	theme	isolated	104:111	arg1	strain					120:125	locally isolated fungal strain	96:125	locally isolated fungal strain	96:125	Influence of medium composition and physical factors on enhanced production of endoglucanase by locally isolated fungal strain in solid state fermentation.
29729694	4	17	attach	isolated	651:658	arg1	sources					679:685	the different sources	665:685	the different sources	665:685	In the current research different thermophilic fungal strains were isolated from the different sources.
29729694	4	17	attach	isolated	651:658	arg2	strains					638:644	different thermophilic fungal strains	608:644	different thermophilic fungal strains	608:644	In the current research different thermophilic fungal strains were isolated from the different sources.
29729694	8	18	theme	EG	1058:1059	arg1	production					1061:1070	maximum EG production	1050:1070	maximum EG production	1050:1070	Different fermentation media were evaluated and M 2 containing wheat bran gave maximum EG production.
29729694	8	19	theme	M	1019:1019	arg1	bran					1040:1043	M 2 containing wheat bran	1019:1043	M 2 containing wheat bran	1019:1043	Different fermentation media were evaluated and M 2 containing wheat bran gave maximum EG production.
29729694	2	20	theme	catalytic	265:273	arg1	hydrolysis					275:284	the catalytic hydrolysis	261:284	the catalytic hydrolysis of cellulose	261:297	Endoglucanase are involved in the catalytic hydrolysis of cellulose and plays a pivotal role in different sectors like pharmaceutical, textile, detergent, and food processing as well as paper and pulp industry.
29729694	2	21	theme	pulp	427:430	arg1	industry					432:439	pulp industry	427:439	pulp industry	427:439	Endoglucanase are involved in the catalytic hydrolysis of cellulose and plays a pivotal role in different sectors like pharmaceutical, textile, detergent, and food processing as well as paper and pulp industry.
29729694	6	22	theme	quantitative	773:784	arg1	screening					786:794	The quantitative screening	769:794	The quantitative screening	769:794	The quantitative screening was carried out employing solid state fermentation.
29729694	2	23	theme	pivotal	311:317	arg1	role					319:322	a pivotal role	309:322	a pivotal role	309:322	Endoglucanase are involved in the catalytic hydrolysis of cellulose and plays a pivotal role in different sectors like pharmaceutical, textile, detergent, and food processing as well as paper and pulp industry.
29729694	7	24	theme	fungal	852:857	arg1	culture					859:865	The fungal culture	848:865	The fungal culture	848:865	The fungal culture, showing highest EG potential was selected identified and assigned the code Aspergillus fumigatus BBT2.
29729694	6	25	theme	solid	822:826	arg1	fermentation					834:845	solid state fermentation	822:845	solid state fermentation	822:845	The quantitative screening was carried out employing solid state fermentation.
29729694	8	26	theme	containing	1023:1032	arg1	bran					1040:1043	M 2 containing wheat bran	1019:1043	M 2 containing wheat bran	1019:1043	Different fermentation media were evaluated and M 2 containing wheat bran gave maximum EG production.
29729694	10	27	theme	best	1240:1243	arg1	carbon					1245:1250	carbon	1245:1250	carbon	1245:1250	Glucose (1%) and peptone (1%) were optimized as best carbon and nitrogen sources, respectively.
29729694	10	27	theme	best	1240:1243	arg1	Glucose					1192:1198	Glucose	1192:1198	Glucose (1%)	1192:1203	Glucose (1%) and peptone (1%) were optimized as best carbon and nitrogen sources, respectively.
29729694	10	27	theme	best	1240:1243	arg1	peptone					1209:1215	peptone	1209:1215	peptone (1%)	1209:1220	Glucose (1%) and peptone (1%) were optimized as best carbon and nitrogen sources, respectively.
29729694	0	28	theme	composition	20:30	arg1	Influence					0:8	Influence	0:8	Influence of medium composition and physical factors on enhanced production of endoglucanase by locally isolated fungal strain in solid state fermentation.	0:154	Influence of medium composition and physical factors on enhanced production of endoglucanase by locally isolated fungal strain in solid state fermentation.
29729694	5	29	theme	Qualitative	688:698	arg1	screening					700:708	Qualitative screening	688:708	Qualitative screening	688:708	Qualitative screening was carried out on the basis of cellulose hydrolysis zone.
29729694	1	30	theme	important	189:197	arg1	enzymes					199:205	the most important enzymes	180:205	the most important enzymes of the cellulase group	180:228	Endoglucanase is one of the most important enzymes of the cellulase group.
29729694	0	31	theme	medium	13:18	arg1	composition					20:30	medium composition	13:30	medium composition	13:30	Influence of medium composition and physical factors on enhanced production of endoglucanase by locally isolated fungal strain in solid state fermentation.
29729694	1	32	theme	enzymes	199:205	arg1	enzymes					199:205	the most important enzymes	180:205	the most important enzymes of the cellulase group	180:228	Endoglucanase is one of the most important enzymes of the cellulase group.
29729694	1	32	theme	enzymes	199:205	arg1	one					173:175	one	173:175	one	173:175	Endoglucanase is one of the most important enzymes of the cellulase group.
29729694	0	33	theme	physical	36:43	arg1	factors					45:51	physical factors	36:51	physical factors	36:51	Influence of medium composition and physical factors on enhanced production of endoglucanase by locally isolated fungal strain in solid state fermentation.
29729694	0	34	theme	state	136:140	arg1	fermentation					142:153	solid state fermentation	130:153	solid state fermentation	130:153	Influence of medium composition and physical factors on enhanced production of endoglucanase by locally isolated fungal strain in solid state fermentation.
29729694	3	35	theme	chemical	564:571	arg1	catalysts					573:581	other chemical catalysts	558:581	other chemical catalysts	558:581	With consumers getting more and more aware of environmental issues, industries find enzymes as a better option over other chemical catalysts.
29729694	4	36	theme	fungal	631:636	arg1	strains					638:644	different thermophilic fungal strains	608:644	different thermophilic fungal strains	608:644	In the current research different thermophilic fungal strains were isolated from the different sources.
29729694	4	37	theme	different	669:677	arg1	sources					679:685	the different sources	665:685	the different sources	665:685	In the current research different thermophilic fungal strains were isolated from the different sources.
29729694	0	38	theme	solid	130:134	arg1	fermentation					142:153	solid state fermentation	130:153	solid state fermentation	130:153	Influence of medium composition and physical factors on enhanced production of endoglucanase by locally isolated fungal strain in solid state fermentation.
29729694	0	39	from	production	65:74	arg1	fermentation					142:153	solid state fermentation	130:153	solid state fermentation	130:153	Influence of medium composition and physical factors on enhanced production of endoglucanase by locally isolated fungal strain in solid state fermentation.
29729694	2	40	theme	food	390:393	arg1	processing					395:404	food processing	390:404	food processing	390:404	Endoglucanase are involved in the catalytic hydrolysis of cellulose and plays a pivotal role in different sectors like pharmaceutical, textile, detergent, and food processing as well as paper and pulp industry.
29729694	5	41	theme	cellulose	742:750	arg1	zone					763:766	cellulose hydrolysis zone	742:766	cellulose hydrolysis zone	742:766	Qualitative screening was carried out on the basis of cellulose hydrolysis zone.
29729694	10	42	theme	nitrogen	1256:1263	arg1	sources					1265:1271	nitrogen sources	1256:1271	nitrogen sources	1256:1271	Glucose (1%) and peptone (1%) were optimized as best carbon and nitrogen sources, respectively.
29729694	10	42	theme	nitrogen	1256:1263	arg1	Glucose					1192:1198	Glucose	1192:1198	Glucose (1%)	1192:1203	Glucose (1%) and peptone (1%) were optimized as best carbon and nitrogen sources, respectively.
29729694	10	42	theme	nitrogen	1256:1263	arg1	peptone					1209:1215	peptone	1209:1215	peptone (1%)	1209:1220	Glucose (1%) and peptone (1%) were optimized as best carbon and nitrogen sources, respectively.
29729694	0	43	theme	factors	45:51	arg1	Influence					0:8	Influence	0:8	Influence of medium composition and physical factors on enhanced production of endoglucanase by locally isolated fungal strain in solid state fermentation.	0:154	Influence of medium composition and physical factors on enhanced production of endoglucanase by locally isolated fungal strain in solid state fermentation.
29729694	3	44	theme	other	558:562	arg1	catalysts					573:581	other chemical catalysts	558:581	other chemical catalysts	558:581	With consumers getting more and more aware of environmental issues, industries find enzymes as a better option over other chemical catalysts.
29729694	2	45	theme	different	327:335	arg1	sectors					337:343	different sectors	327:343	different sectors like pharmaceutical, textile, detergent, and food processing	327:404	Endoglucanase are involved in the catalytic hydrolysis of cellulose and plays a pivotal role in different sectors like pharmaceutical, textile, detergent, and food processing as well as paper and pulp industry.
29729694	8	46	theme	fermentation	981:992	arg1	media					994:998	Different fermentation media	971:998	Different fermentation media	971:998	Different fermentation media were evaluated and M 2 containing wheat bran gave maximum EG production.
29729694	9	47	theme	inoculum	1143:1150	arg1	1.5ml					1157:1161	inoculum size 1.5ml	1143:1161	inoculum size 1.5ml	1143:1161	The maximal enzyme productivity was recorded in 72 hours, 40°C, pH 5, inoculum size 1.5ml, and moisture content (1:1).
29729694	9	47	theme	inoculum	1143:1150	arg1	hours					1124:1128	72 hours	1121:1128	72 hours	1121:1128	The maximal enzyme productivity was recorded in 72 hours, 40°C, pH 5, inoculum size 1.5ml, and moisture content (1:1).
29729694	1	48	theme	cellulase	214:222	arg1	group					224:228	the cellulase group	210:228	the cellulase group	210:228	Endoglucanase is one of the most important enzymes of the cellulase group.
29729694	6	49	theme	state	828:832	arg1	fermentation					834:845	solid state fermentation	822:845	solid state fermentation	822:845	The quantitative screening was carried out employing solid state fermentation.
29729694	7	50	theme	Aspergillus	943:953	arg1	BBT2					965:968	Aspergillus fumigatus BBT2	943:968	Aspergillus fumigatus BBT2	943:968	The fungal culture, showing highest EG potential was selected identified and assigned the code Aspergillus fumigatus BBT2.
29729694	1	51	theme	group	224:228	arg1	enzymes					199:205	the most important enzymes	180:205	the most important enzymes of the cellulase group	180:228	Endoglucanase is one of the most important enzymes of the cellulase group.
29729694	4	52	theme	thermophilic	618:629	arg1	strains					638:644	different thermophilic fungal strains	608:644	different thermophilic fungal strains	608:644	In the current research different thermophilic fungal strains were isolated from the different sources.
29729694	4	53	theme	different	608:616	arg1	strains					638:644	different thermophilic fungal strains	608:644	different thermophilic fungal strains	608:644	In the current research different thermophilic fungal strains were isolated from the different sources.
29729694	3	54	theme	environmental	488:500	arg1	issues					502:507	environmental issues	488:507	environmental issues	488:507	With consumers getting more and more aware of environmental issues, industries find enzymes as a better option over other chemical catalysts.
29729694	4	55	theme	current	591:597	arg1	research					599:606	the current research	587:606	the current research	587:606	In the current research different thermophilic fungal strains were isolated from the different sources.
29729694	0	56	theme	enhanced	56:63	arg1	production					65:74	enhanced production	56:74	enhanced production of endoglucanase by locally isolated fungal strain in solid state fermentation	56:153	Influence of medium composition and physical factors on enhanced production of endoglucanase by locally isolated fungal strain in solid state fermentation.
29729694	8	57	theme	Different	971:979	arg1	media					994:998	Different fermentation media	971:998	Different fermentation media	971:998	Different fermentation media were evaluated and M 2 containing wheat bran gave maximum EG production.
29729694	3	58	theme	issues	502:507	arg1	aware					479:483	aware	479:483	aware of environmental issues	479:507	With consumers getting more and more aware of environmental issues, industries find enzymes as a better option over other chemical catalysts.
29729694	7	59	theme	EG	884:885	arg1	potential					887:895	highest EG potential	876:895	highest EG potential	876:895	The fungal culture, showing highest EG potential was selected identified and assigned the code Aspergillus fumigatus BBT2.
31342578	0	0	theme	cis-prenyltransferase-like	78:103	arg1	protein					107:113	cis-prenyltransferase-like 1 protein	78:113	cis-prenyltransferase-like 1 protein	78:113	Uncovering mechanisms of rubber biosynthesis in Taraxacum koksaghyz - role of cis-prenyltransferase-like 1 protein.
31342578	4	1	dep	catalyzes	574:582	arg1	Located					450:456	Located	450:456	Located	450:456	Located at the particle surface, the rubber transferase complex, member of the cis-prenyltransferase (cisPT) enzyme family, catalyzes the elongation of the rubber chains.
31342578	1	2	from	rubber	216:221	arg1	roots					230:234	its roots	226:234	its roots	226:234	The Russian dandelion Taraxacum koksaghyz synthesizes considerable amounts of high-molecular-weight rubber in its roots.
31342578	10	3	theme	shell	1792:1796	arg1	components					1758:1767	components	1758:1767	components of the rubber particle shell	1758:1796	MS-based comparison of latex proteomes from TkCPTL1-RNAi plants and T. koksaghyz wild-types discovered putative factors that convert metabolites in biosynthetic pathways connected to isoprenoids or that synthesize components of the rubber particle shell.
31342578	9	4	theme	low-expressed	1431:1443	arg1	TkCPTL2					1445:1451	the low-expressed TkCPTL2	1427:1451	the low-expressed TkCPTL2	1427:1451	Conversely, downregulating the low-expressed TkCPTL2 showed no altered phenotype, suggesting its protein function is redundant in T. koksaghyz.
31342578	7	5	theme	phospholipid	1220:1231	arg1	composition					1233:1243	phospholipid composition	1220:1243	phospholipid composition	1220:1243	Analyses of latex from these TkCPTL1-RNAi plants revealed particles that were similar to native rubber particles regarding their particle size, phospholipid composition, and presence of small rubber particle proteins (SRPPs).
31342578	0	6	from	mechanisms	11:20	arg1	role					70:73	role	70:73	role	70:73	Uncovering mechanisms of rubber biosynthesis in Taraxacum koksaghyz - role of cis-prenyltransferase-like 1 protein.
31342578	0	6	from	mechanisms	11:20	arg1	koksaghyz					58:66	Taraxacum koksaghyz	48:66	Taraxacum koksaghyz	48:66	Uncovering mechanisms of rubber biosynthesis in Taraxacum koksaghyz - role of cis-prenyltransferase-like 1 protein.
31342578	1	7	from	amounts	183:189	arg1	roots					230:234	its roots	226:234	its roots	226:234	The Russian dandelion Taraxacum koksaghyz synthesizes considerable amounts of high-molecular-weight rubber in its roots.
31342578	10	8	theme	particle	1783:1790	arg1	shell					1792:1796	the rubber particle shell	1772:1796	the rubber particle shell	1772:1796	MS-based comparison of latex proteomes from TkCPTL1-RNAi plants and T. koksaghyz wild-types discovered putative factors that convert metabolites in biosynthetic pathways connected to isoprenoids or that synthesize components of the rubber particle shell.
31342578	10	9	theme	TkCPTL1-RNAi	1588:1599	arg1	plants					1601:1606	TkCPTL1-RNAi plants	1588:1606	TkCPTL1-RNAi plants	1588:1606	MS-based comparison of latex proteomes from TkCPTL1-RNAi plants and T. koksaghyz wild-types discovered putative factors that convert metabolites in biosynthetic pathways connected to isoprenoids or that synthesize components of the rubber particle shell.
31342578	4	10	theme	chains	613:618	arg1	elongation					588:597	the elongation	584:597	the elongation of the rubber chains	584:618	Located at the particle surface, the rubber transferase complex, member of the cis-prenyltransferase (cisPT) enzyme family, catalyzes the elongation of the rubber chains.
31342578	0	11	theme	protein	107:113	arg1	koksaghyz					58:66	Taraxacum koksaghyz	48:66	Taraxacum koksaghyz	48:66	Uncovering mechanisms of rubber biosynthesis in Taraxacum koksaghyz - role of cis-prenyltransferase-like 1 protein.
31342578	5	12	theme	transferase	638:648	arg1	heteromer					650:658	An active rubber transferase heteromer	621:658	An active rubber transferase heteromer	621:658	An active rubber transferase heteromer requires a cisPT subunit (CPT) as well as a CPT-like subunit (CPTL), of which T. koksaghyz has two homologous forms: TkCPTL1 and TkCPTL2, which potentially associate with the rubber transferase complex.
31342578	10	13	theme	T. koksaghyz	1612:1623	arg1	wild-types					1625:1634	T. koksaghyz wild-types	1612:1634	T. koksaghyz wild-types	1612:1634	MS-based comparison of latex proteomes from TkCPTL1-RNAi plants and T. koksaghyz wild-types discovered putative factors that convert metabolites in biosynthetic pathways connected to isoprenoids or that synthesize components of the rubber particle shell.
31342578	6	14	theme	unaffected	982:991	arg1	content					1002:1008	unaffected dolichol content	982:1008	unaffected dolichol content	982:1008	Knockdown of TkCPTL1, which is predominantly expressed in latex, led to abolished poly(cis-1,4-isoprene) synthesis but unaffected dolichol content, whereas levels of triterpenes and inulin were elevated in roots.
31342578	7	15	theme	rubber	1268:1273	arg1	SRPPs					1294:1298	SRPPs	1294:1298	SRPPs	1294:1298	Analyses of latex from these TkCPTL1-RNAi plants revealed particles that were similar to native rubber particles regarding their particle size, phospholipid composition, and presence of small rubber particle proteins (SRPPs).
31342578	7	15	theme	rubber	1268:1273	arg1	proteins					1284:1291	small rubber particle proteins	1262:1291	small rubber particle proteins (SRPPs)	1262:1299	Analyses of latex from these TkCPTL1-RNAi plants revealed particles that were similar to native rubber particles regarding their particle size, phospholipid composition, and presence of small rubber particle proteins (SRPPs).
31342578	5	16	contain	has	751:753	arg1	T. koksaghyz					738:749	T. koksaghyz	738:749	T. koksaghyz	738:749	An active rubber transferase heteromer requires a cisPT subunit (CPT) as well as a CPT-like subunit (CPTL), of which T. koksaghyz has two homologous forms: TkCPTL1 and TkCPTL2, which potentially associate with the rubber transferase complex.
31342578	5	16	contain	has	751:753	arg2	TkCPTL1					777:783	TkCPTL1	777:783	TkCPTL1	777:783	An active rubber transferase heteromer requires a cisPT subunit (CPT) as well as a CPT-like subunit (CPTL), of which T. koksaghyz has two homologous forms: TkCPTL1 and TkCPTL2, which potentially associate with the rubber transferase complex.
31342578	5	16	contain	has	751:753	arg2	forms					770:774	two homologous forms	755:774	two homologous forms: TkCPTL1 and TkCPTL2	755:795	An active rubber transferase heteromer requires a cisPT subunit (CPT) as well as a CPT-like subunit (CPTL), of which T. koksaghyz has two homologous forms: TkCPTL1 and TkCPTL2, which potentially associate with the rubber transferase complex.
31342578	5	16	contain	has	751:753	arg2	TkCPTL2					789:795	TkCPTL2	789:795	TkCPTL2	789:795	An active rubber transferase heteromer requires a cisPT subunit (CPT) as well as a CPT-like subunit (CPTL), of which T. koksaghyz has two homologous forms: TkCPTL1 and TkCPTL2, which potentially associate with the rubber transferase complex.
31342578	7	17	theme	TkCPTL1-RNAi	1105:1116	arg1	plants					1118:1123	these TkCPTL1-RNAi plants	1099:1123	these TkCPTL1-RNAi plants	1099:1123	Analyses of latex from these TkCPTL1-RNAi plants revealed particles that were similar to native rubber particles regarding their particle size, phospholipid composition, and presence of small rubber particle proteins (SRPPs).
31342578	9	18	from	T. koksaghyz	1530:1541	arg1	redundant					1517:1525	redundant	1517:1525	redundant	1517:1525	Conversely, downregulating the low-expressed TkCPTL2 showed no altered phenotype, suggesting its protein function is redundant in T. koksaghyz.
31342578	10	19	theme	rubber	1776:1781	arg1	shell					1792:1796	the rubber particle shell	1772:1796	the rubber particle shell	1772:1796	MS-based comparison of latex proteomes from TkCPTL1-RNAi plants and T. koksaghyz wild-types discovered putative factors that convert metabolites in biosynthetic pathways connected to isoprenoids or that synthesize components of the rubber particle shell.
31342578	7	20	theme	proteins	1284:1291	arg1	size					1214:1217	particle size	1205:1217	particle size	1205:1217	Analyses of latex from these TkCPTL1-RNAi plants revealed particles that were similar to native rubber particles regarding their particle size, phospholipid composition, and presence of small rubber particle proteins (SRPPs).
31342578	7	20	theme	proteins	1284:1291	arg1	composition					1233:1243	phospholipid composition	1220:1243	phospholipid composition	1220:1243	Analyses of latex from these TkCPTL1-RNAi plants revealed particles that were similar to native rubber particles regarding their particle size, phospholipid composition, and presence of small rubber particle proteins (SRPPs).
31342578	7	20	theme	proteins	1284:1291	arg1	presence					1250:1257	presence	1250:1257	presence of small rubber particle proteins (SRPPs)	1250:1299	Analyses of latex from these TkCPTL1-RNAi plants revealed particles that were similar to native rubber particles regarding their particle size, phospholipid composition, and presence of small rubber particle proteins (SRPPs).
31342578	7	21	theme	latex	1088:1092	arg1	Analyses					1076:1083	Analyses	1076:1083	Analyses of latex from these TkCPTL1-RNAi plants	1076:1123	Analyses of latex from these TkCPTL1-RNAi plants revealed particles that were similar to native rubber particles regarding their particle size, phospholipid composition, and presence of small rubber particle proteins (SRPPs).
31342578	6	22	theme	inulin	1045:1050	arg1	levels					1019:1024	levels	1019:1024	levels of triterpenes and inulin	1019:1050	Knockdown of TkCPTL1, which is predominantly expressed in latex, led to abolished poly(cis-1,4-isoprene) synthesis but unaffected dolichol content, whereas levels of triterpenes and inulin were elevated in roots.
31342578	1	23	theme	high-molecular-weight	194:214	arg1	rubber					216:221	high-molecular-weight rubber	194:221	high-molecular-weight rubber in its roots	194:234	The Russian dandelion Taraxacum koksaghyz synthesizes considerable amounts of high-molecular-weight rubber in its roots.
31342578	3	24	theme	cis-1,4-isoprene	392:407	arg1	polymers					409:416	The cis-1,4-isoprene polymers	388:416	The cis-1,4-isoprene polymers	388:416	The cis-1,4-isoprene polymers are stored in rubber particles.
31342578	4	25	theme	cis-prenyltransferase	529:549	arg1	family					566:571	the cis-prenyltransferase (cisPT) enzyme family	525:571	the cis-prenyltransferase (cisPT) enzyme family	525:571	Located at the particle surface, the rubber transferase complex, member of the cis-prenyltransferase (cisPT) enzyme family, catalyzes the elongation of the rubber chains.
31342578	5	26	theme	cisPT	671:675	arg1	subunit					677:683	a cisPT subunit	669:683	a cisPT subunit (CPT) as well as a CPT-like subunit (CPTL), of which T. koksaghyz has two homologous forms: TkCPTL1 and TkCPTL2, which potentially associate with the rubber transferase complex	669:860	An active rubber transferase heteromer requires a cisPT subunit (CPT) as well as a CPT-like subunit (CPTL), of which T. koksaghyz has two homologous forms: TkCPTL1 and TkCPTL2, which potentially associate with the rubber transferase complex.
31342578	5	26	theme	cisPT	671:675	arg1	CPT					686:688	CPT	686:688	CPT	686:688	An active rubber transferase heteromer requires a cisPT subunit (CPT) as well as a CPT-like subunit (CPTL), of which T. koksaghyz has two homologous forms: TkCPTL1 and TkCPTL2, which potentially associate with the rubber transferase complex.
31342578	9	27	from	redundant	1517:1525	arg1	T. koksaghyz					1530:1541	T. koksaghyz	1530:1541	T. koksaghyz	1530:1541	Conversely, downregulating the low-expressed TkCPTL2 showed no altered phenotype, suggesting its protein function is redundant in T. koksaghyz.
31342578	10	28	theme	putative	1647:1654	arg1	factors					1656:1662	putative factors	1647:1662	putative factors that convert metabolites in biosynthetic pathways connected to isoprenoids or that synthesize components of the rubber particle shell	1647:1796	MS-based comparison of latex proteomes from TkCPTL1-RNAi plants and T. koksaghyz wild-types discovered putative factors that convert metabolites in biosynthetic pathways connected to isoprenoids or that synthesize components of the rubber particle shell.
31342578	1	29	theme	rubber	216:221	arg1	amounts					183:189	considerable amounts	170:189	considerable amounts of high-molecular-weight rubber in its roots	170:234	The Russian dandelion Taraxacum koksaghyz synthesizes considerable amounts of high-molecular-weight rubber in its roots.
31342578	1	29	theme	rubber	216:221	arg1	rubber					216:221	high-molecular-weight rubber	194:221	high-molecular-weight rubber in its roots	194:234	The Russian dandelion Taraxacum koksaghyz synthesizes considerable amounts of high-molecular-weight rubber in its roots.
31342578	3	30	theme	rubber	432:437	arg1	particles					439:447	rubber particles	432:447	rubber particles	432:447	The cis-1,4-isoprene polymers are stored in rubber particles.
31342578	10	31	from	metabolites	1677:1687	arg1	pathways					1705:1712	biosynthetic pathways	1692:1712	biosynthetic pathways connected to isoprenoids	1692:1737	MS-based comparison of latex proteomes from TkCPTL1-RNAi plants and T. koksaghyz wild-types discovered putative factors that convert metabolites in biosynthetic pathways connected to isoprenoids or that synthesize components of the rubber particle shell.
31342578	7	32	theme	rubber	1172:1177	arg1	particles					1179:1187	native rubber particles	1165:1187	native rubber particles regarding their particle size, phospholipid composition, and presence of small rubber particle proteins (SRPPs)	1165:1299	Analyses of latex from these TkCPTL1-RNAi plants revealed particles that were similar to native rubber particles regarding their particle size, phospholipid composition, and presence of small rubber particle proteins (SRPPs).
31342578	9	33	theme	altered	1463:1469	arg1	phenotype					1471:1479	no altered phenotype	1460:1479	no altered phenotype	1460:1479	Conversely, downregulating the low-expressed TkCPTL2 showed no altered phenotype, suggesting its protein function is redundant in T. koksaghyz.
31342578	4	34	theme	enzyme	559:564	arg1	family					566:571	the cis-prenyltransferase (cisPT) enzyme family	525:571	the cis-prenyltransferase (cisPT) enzyme family	525:571	Located at the particle surface, the rubber transferase complex, member of the cis-prenyltransferase (cisPT) enzyme family, catalyzes the elongation of the rubber chains.
31342578	2	35	theme	T. koksaghyz	357:368	arg1	establishment					340:352	the establishment	336:352	the establishment of T. koksaghyz as a rubber crop	336:385	The characterization of factors that participate in natural rubber biosynthesis is fundamental for the establishment of T. koksaghyz as a rubber crop.
31342578	10	36	theme	biosynthetic	1692:1703	arg1	pathways					1705:1712	biosynthetic pathways	1692:1712	biosynthetic pathways connected to isoprenoids	1692:1737	MS-based comparison of latex proteomes from TkCPTL1-RNAi plants and T. koksaghyz wild-types discovered putative factors that convert metabolites in biosynthetic pathways connected to isoprenoids or that synthesize components of the rubber particle shell.
31342578	0	37	theme	rubber	25:30	arg1	biosynthesis					32:43	rubber biosynthesis	25:43	rubber biosynthesis	25:43	Uncovering mechanisms of rubber biosynthesis in Taraxacum koksaghyz - role of cis-prenyltransferase-like 1 protein.
31342578	5	38	dep	forms	770:774	arg1	TkCPTL2					789:795	TkCPTL2	789:795	TkCPTL2	789:795	An active rubber transferase heteromer requires a cisPT subunit (CPT) as well as a CPT-like subunit (CPTL), of which T. koksaghyz has two homologous forms: TkCPTL1 and TkCPTL2, which potentially associate with the rubber transferase complex.
31342578	5	38	dep	forms	770:774	arg1	TkCPTL1					777:783	TkCPTL1	777:783	TkCPTL1	777:783	An active rubber transferase heteromer requires a cisPT subunit (CPT) as well as a CPT-like subunit (CPTL), of which T. koksaghyz has two homologous forms: TkCPTL1 and TkCPTL2, which potentially associate with the rubber transferase complex.
31342578	5	38	dep	forms	770:774	arg1	forms					770:774	two homologous forms	755:774	two homologous forms: TkCPTL1 and TkCPTL2	755:795	An active rubber transferase heteromer requires a cisPT subunit (CPT) as well as a CPT-like subunit (CPTL), of which T. koksaghyz has two homologous forms: TkCPTL1 and TkCPTL2, which potentially associate with the rubber transferase complex.
31342578	5	39	theme	active	624:629	arg1	heteromer					650:658	An active rubber transferase heteromer	621:658	An active rubber transferase heteromer	621:658	An active rubber transferase heteromer requires a cisPT subunit (CPT) as well as a CPT-like subunit (CPTL), of which T. koksaghyz has two homologous forms: TkCPTL1 and TkCPTL2, which potentially associate with the rubber transferase complex.
31342578	5	40	theme	rubber	631:636	arg1	heteromer					650:658	An active rubber transferase heteromer	621:658	An active rubber transferase heteromer	621:658	An active rubber transferase heteromer requires a cisPT subunit (CPT) as well as a CPT-like subunit (CPTL), of which T. koksaghyz has two homologous forms: TkCPTL1 and TkCPTL2, which potentially associate with the rubber transferase complex.
31342578	7	41	theme	native	1165:1170	arg1	particles					1179:1187	native rubber particles	1165:1187	native rubber particles regarding their particle size, phospholipid composition, and presence of small rubber particle proteins (SRPPs)	1165:1299	Analyses of latex from these TkCPTL1-RNAi plants revealed particles that were similar to native rubber particles regarding their particle size, phospholipid composition, and presence of small rubber particle proteins (SRPPs).
31342578	4	42	theme	rubber	606:611	arg1	chains					613:618	the rubber chains	602:618	the rubber chains	602:618	Located at the particle surface, the rubber transferase complex, member of the cis-prenyltransferase (cisPT) enzyme family, catalyzes the elongation of the rubber chains.
31342578	4	43	theme	transferase	494:504	arg1	member					515:520	member	515:520	member of the cis-prenyltransferase (cisPT) enzyme family	515:571	Located at the particle surface, the rubber transferase complex, member of the cis-prenyltransferase (cisPT) enzyme family, catalyzes the elongation of the rubber chains.
31342578	4	43	theme	transferase	494:504	arg1	complex					506:512	the rubber transferase complex	483:512	the rubber transferase complex	483:512	Located at the particle surface, the rubber transferase complex, member of the cis-prenyltransferase (cisPT) enzyme family, catalyzes the elongation of the rubber chains.
31342578	5	44	theme	rubber	835:840	arg1	complex					854:860	the rubber transferase complex	831:860	the rubber transferase complex	831:860	An active rubber transferase heteromer requires a cisPT subunit (CPT) as well as a CPT-like subunit (CPTL), of which T. koksaghyz has two homologous forms: TkCPTL1 and TkCPTL2, which potentially associate with the rubber transferase complex.
31342578	7	45	theme	particle	1205:1212	arg1	size					1214:1217	particle size	1205:1217	particle size	1205:1217	Analyses of latex from these TkCPTL1-RNAi plants revealed particles that were similar to native rubber particles regarding their particle size, phospholipid composition, and presence of small rubber particle proteins (SRPPs).
31342578	4	46	theme	rubber	487:492	arg1	member					515:520	member	515:520	member of the cis-prenyltransferase (cisPT) enzyme family	515:571	Located at the particle surface, the rubber transferase complex, member of the cis-prenyltransferase (cisPT) enzyme family, catalyzes the elongation of the rubber chains.
31342578	4	46	theme	rubber	487:492	arg1	complex					506:512	the rubber transferase complex	483:512	the rubber transferase complex	483:512	Located at the particle surface, the rubber transferase complex, member of the cis-prenyltransferase (cisPT) enzyme family, catalyzes the elongation of the rubber chains.
31342578	5	47	theme	transferase	842:852	arg1	complex					854:860	the rubber transferase complex	831:860	the rubber transferase complex	831:860	An active rubber transferase heteromer requires a cisPT subunit (CPT) as well as a CPT-like subunit (CPTL), of which T. koksaghyz has two homologous forms: TkCPTL1 and TkCPTL2, which potentially associate with the rubber transferase complex.
31342578	0	48	theme	biosynthesis	32:43	arg1	mechanisms					11:20	mechanisms	11:20	mechanisms of rubber biosynthesis in Taraxacum koksaghyz - role of cis-prenyltransferase-like 1 protein	11:113	Uncovering mechanisms of rubber biosynthesis in Taraxacum koksaghyz - role of cis-prenyltransferase-like 1 protein.
31342578	6	49	theme	poly	945:948	arg1	synthesis					968:976	abolished poly(cis-1,4-isoprene) synthesis	935:976	abolished poly(cis-1,4-isoprene) synthesis	935:976	Knockdown of TkCPTL1, which is predominantly expressed in latex, led to abolished poly(cis-1,4-isoprene) synthesis but unaffected dolichol content, whereas levels of triterpenes and inulin were elevated in roots.
31342578	6	50	theme	dolichol	993:1000	arg1	content					1002:1008	unaffected dolichol content	982:1008	unaffected dolichol content	982:1008	Knockdown of TkCPTL1, which is predominantly expressed in latex, led to abolished poly(cis-1,4-isoprene) synthesis but unaffected dolichol content, whereas levels of triterpenes and inulin were elevated in roots.
31342578	10	51	from	plants	1601:1606	arg1	comparison					1553:1562	MS-based comparison	1544:1562	MS-based comparison of latex proteomes from TkCPTL1-RNAi plants and T. koksaghyz wild-types	1544:1634	MS-based comparison of latex proteomes from TkCPTL1-RNAi plants and T. koksaghyz wild-types discovered putative factors that convert metabolites in biosynthetic pathways connected to isoprenoids or that synthesize components of the rubber particle shell.
31342578	6	52	theme	triterpenes	1029:1039	arg1	levels					1019:1024	levels	1019:1024	levels of triterpenes and inulin	1019:1050	Knockdown of TkCPTL1, which is predominantly expressed in latex, led to abolished poly(cis-1,4-isoprene) synthesis but unaffected dolichol content, whereas levels of triterpenes and inulin were elevated in roots.
31342578	10	53	from	wild-types	1625:1634	arg1	comparison					1553:1562	MS-based comparison	1544:1562	MS-based comparison of latex proteomes from TkCPTL1-RNAi plants and T. koksaghyz wild-types	1544:1634	MS-based comparison of latex proteomes from TkCPTL1-RNAi plants and T. koksaghyz wild-types discovered putative factors that convert metabolites in biosynthetic pathways connected to isoprenoids or that synthesize components of the rubber particle shell.
31342578	4	54	theme	family	566:571	arg1	member					515:520	member	515:520	member of the cis-prenyltransferase (cisPT) enzyme family	515:571	Located at the particle surface, the rubber transferase complex, member of the cis-prenyltransferase (cisPT) enzyme family, catalyzes the elongation of the rubber chains.
31342578	4	54	theme	family	566:571	arg1	complex					506:512	the rubber transferase complex	483:512	the rubber transferase complex	483:512	Located at the particle surface, the rubber transferase complex, member of the cis-prenyltransferase (cisPT) enzyme family, catalyzes the elongation of the rubber chains.
31342578	10	55	theme	proteomes	1573:1581	arg1	comparison					1553:1562	MS-based comparison	1544:1562	MS-based comparison of latex proteomes from TkCPTL1-RNAi plants and T. koksaghyz wild-types	1544:1634	MS-based comparison of latex proteomes from TkCPTL1-RNAi plants and T. koksaghyz wild-types discovered putative factors that convert metabolites in biosynthetic pathways connected to isoprenoids or that synthesize components of the rubber particle shell.
31342578	2	56	theme	factors	261:267	arg1	fundamental					320:330	fundamental	320:330	fundamental	320:330	The characterization of factors that participate in natural rubber biosynthesis is fundamental for the establishment of T. koksaghyz as a rubber crop.
31342578	2	56	theme	factors	261:267	arg1	characterization					241:256	The characterization	237:256	The characterization of factors that participate in natural rubber biosynthesis	237:315	The characterization of factors that participate in natural rubber biosynthesis is fundamental for the establishment of T. koksaghyz as a rubber crop.
31342578	5	57	theme	CPT-like	704:711	arg1	CPTL					722:725	CPTL	722:725	CPTL	722:725	An active rubber transferase heteromer requires a cisPT subunit (CPT) as well as a CPT-like subunit (CPTL), of which T. koksaghyz has two homologous forms: TkCPTL1 and TkCPTL2, which potentially associate with the rubber transferase complex.
31342578	5	57	theme	CPT-like	704:711	arg1	subunit					713:719	a CPT-like subunit	702:719	a CPT-like subunit (CPTL)	702:726	An active rubber transferase heteromer requires a cisPT subunit (CPT) as well as a CPT-like subunit (CPTL), of which T. koksaghyz has two homologous forms: TkCPTL1 and TkCPTL2, which potentially associate with the rubber transferase complex.
31342578	1	58	theme	Russian	120:126	arg1	koksaghyz					148:156	The Russian dandelion Taraxacum koksaghyz	116:156	The Russian dandelion Taraxacum koksaghyz	116:156	The Russian dandelion Taraxacum koksaghyz synthesizes considerable amounts of high-molecular-weight rubber in its roots.
31342578	0	59	theme	Taraxacum	48:56	arg1	koksaghyz					58:66	Taraxacum koksaghyz	48:66	Taraxacum koksaghyz	48:66	Uncovering mechanisms of rubber biosynthesis in Taraxacum koksaghyz - role of cis-prenyltransferase-like 1 protein.
31342578	7	60	from	plants	1118:1123	arg1	Analyses					1076:1083	Analyses	1076:1083	Analyses of latex from these TkCPTL1-RNAi plants	1076:1123	Analyses of latex from these TkCPTL1-RNAi plants revealed particles that were similar to native rubber particles regarding their particle size, phospholipid composition, and presence of small rubber particle proteins (SRPPs).
31342578	5	61	dep	has	751:753	arg1	associate					816:824	associate	816:824	associate with the rubber transferase complex	816:860	An active rubber transferase heteromer requires a cisPT subunit (CPT) as well as a CPT-like subunit (CPTL), of which T. koksaghyz has two homologous forms: TkCPTL1 and TkCPTL2, which potentially associate with the rubber transferase complex.
31342578	10	62	theme	latex	1567:1571	arg1	proteomes					1573:1581	latex proteomes	1567:1581	latex proteomes	1567:1581	MS-based comparison of latex proteomes from TkCPTL1-RNAi plants and T. koksaghyz wild-types discovered putative factors that convert metabolites in biosynthetic pathways connected to isoprenoids or that synthesize components of the rubber particle shell.
31342578	1	63	from	roots	230:234	arg1	amounts					183:189	considerable amounts	170:189	considerable amounts of high-molecular-weight rubber in its roots	170:234	The Russian dandelion Taraxacum koksaghyz synthesizes considerable amounts of high-molecular-weight rubber in its roots.
31342578	1	63	from	roots	230:234	arg1	rubber					216:221	high-molecular-weight rubber	194:221	high-molecular-weight rubber in its roots	194:234	The Russian dandelion Taraxacum koksaghyz synthesizes considerable amounts of high-molecular-weight rubber in its roots.
31342578	1	64	theme	dandelion	128:136	arg1	koksaghyz					148:156	The Russian dandelion Taraxacum koksaghyz	116:156	The Russian dandelion Taraxacum koksaghyz	116:156	The Russian dandelion Taraxacum koksaghyz synthesizes considerable amounts of high-molecular-weight rubber in its roots.
31342578	7	65	theme	small	1262:1266	arg1	SRPPs					1294:1298	SRPPs	1294:1298	SRPPs	1294:1298	Analyses of latex from these TkCPTL1-RNAi plants revealed particles that were similar to native rubber particles regarding their particle size, phospholipid composition, and presence of small rubber particle proteins (SRPPs).
31342578	7	65	theme	small	1262:1266	arg1	proteins					1284:1291	small rubber particle proteins	1262:1291	small rubber particle proteins (SRPPs)	1262:1299	Analyses of latex from these TkCPTL1-RNAi plants revealed particles that were similar to native rubber particles regarding their particle size, phospholipid composition, and presence of small rubber particle proteins (SRPPs).
31342578	4	66	from	surface	474:480	arg1	Located					450:456	Located	450:456	Located	450:456	Located at the particle surface, the rubber transferase complex, member of the cis-prenyltransferase (cisPT) enzyme family, catalyzes the elongation of the rubber chains.
31342578	4	67	theme	particle	465:472	arg1	surface					474:480	the particle surface	461:480	the particle surface	461:480	Located at the particle surface, the rubber transferase complex, member of the cis-prenyltransferase (cisPT) enzyme family, catalyzes the elongation of the rubber chains.
31342578	8	68	theme	phospholipid	1360:1371	arg1	shell					1373:1377	a phospholipid shell	1358:1377	a phospholipid shell stabilized by SRPPs	1358:1397	We found that the particles encapsulated triterpenes in a phospholipid shell stabilized by SRPPs.
31342578	1	69	theme	Taraxacum	138:146	arg1	koksaghyz					148:156	The Russian dandelion Taraxacum koksaghyz	116:156	The Russian dandelion Taraxacum koksaghyz	116:156	The Russian dandelion Taraxacum koksaghyz synthesizes considerable amounts of high-molecular-weight rubber in its roots.
31342578	6	70	theme	TkCPTL1	876:882	arg1	Knockdown					863:871	Knockdown	863:871	Knockdown of TkCPTL1, which is predominantly expressed in latex,	863:926	Knockdown of TkCPTL1, which is predominantly expressed in latex, led to abolished poly(cis-1,4-isoprene) synthesis but unaffected dolichol content, whereas levels of triterpenes and inulin were elevated in roots.
31342578	2	71	theme	rubber	297:302	arg1	biosynthesis					304:315	natural rubber biosynthesis	289:315	natural rubber biosynthesis	289:315	The characterization of factors that participate in natural rubber biosynthesis is fundamental for the establishment of T. koksaghyz as a rubber crop.
31342578	7	72	theme	particle	1275:1282	arg1	SRPPs					1294:1298	SRPPs	1294:1298	SRPPs	1294:1298	Analyses of latex from these TkCPTL1-RNAi plants revealed particles that were similar to native rubber particles regarding their particle size, phospholipid composition, and presence of small rubber particle proteins (SRPPs).
31342578	7	72	theme	particle	1275:1282	arg1	proteins					1284:1291	small rubber particle proteins	1262:1291	small rubber particle proteins (SRPPs)	1262:1299	Analyses of latex from these TkCPTL1-RNAi plants revealed particles that were similar to native rubber particles regarding their particle size, phospholipid composition, and presence of small rubber particle proteins (SRPPs).
31342578	8	73	from	triterpenes	1343:1353	arg1	shell					1373:1377	a phospholipid shell	1358:1377	a phospholipid shell stabilized by SRPPs	1358:1397	We found that the particles encapsulated triterpenes in a phospholipid shell stabilized by SRPPs.
31342578	2	74	theme	rubber	375:380	arg1	crop					382:385	a rubber crop	373:385	a rubber crop	373:385	The characterization of factors that participate in natural rubber biosynthesis is fundamental for the establishment of T. koksaghyz as a rubber crop.
31342578	9	75	theme	protein	1497:1503	arg1	function					1505:1512	its protein function	1493:1512	its protein function	1493:1512	Conversely, downregulating the low-expressed TkCPTL2 showed no altered phenotype, suggesting its protein function is redundant in T. koksaghyz.
31342578	2	76	theme	natural	289:295	arg1	biosynthesis					304:315	natural rubber biosynthesis	289:315	natural rubber biosynthesis	289:315	The characterization of factors that participate in natural rubber biosynthesis is fundamental for the establishment of T. koksaghyz as a rubber crop.
31342578	10	77	theme	MS-based	1544:1551	arg1	comparison					1553:1562	MS-based comparison	1544:1562	MS-based comparison of latex proteomes from TkCPTL1-RNAi plants and T. koksaghyz wild-types	1544:1634	MS-based comparison of latex proteomes from TkCPTL1-RNAi plants and T. koksaghyz wild-types discovered putative factors that convert metabolites in biosynthetic pathways connected to isoprenoids or that synthesize components of the rubber particle shell.
31342578	5	78	theme	homologous	759:768	arg1	TkCPTL2					789:795	TkCPTL2	789:795	TkCPTL2	789:795	An active rubber transferase heteromer requires a cisPT subunit (CPT) as well as a CPT-like subunit (CPTL), of which T. koksaghyz has two homologous forms: TkCPTL1 and TkCPTL2, which potentially associate with the rubber transferase complex.
31342578	5	78	theme	homologous	759:768	arg1	TkCPTL1					777:783	TkCPTL1	777:783	TkCPTL1	777:783	An active rubber transferase heteromer requires a cisPT subunit (CPT) as well as a CPT-like subunit (CPTL), of which T. koksaghyz has two homologous forms: TkCPTL1 and TkCPTL2, which potentially associate with the rubber transferase complex.
31342578	5	78	theme	homologous	759:768	arg1	forms					770:774	two homologous forms	755:774	two homologous forms: TkCPTL1 and TkCPTL2	755:795	An active rubber transferase heteromer requires a cisPT subunit (CPT) as well as a CPT-like subunit (CPTL), of which T. koksaghyz has two homologous forms: TkCPTL1 and TkCPTL2, which potentially associate with the rubber transferase complex.
31342578	4	79	theme	cisPT	552:556	arg1	family					566:571	the cis-prenyltransferase (cisPT) enzyme family	525:571	the cis-prenyltransferase (cisPT) enzyme family	525:571	Located at the particle surface, the rubber transferase complex, member of the cis-prenyltransferase (cisPT) enzyme family, catalyzes the elongation of the rubber chains.
31342578	9	80	dep	suggesting	1482:1491	arg1	redundant					1517:1525	redundant	1517:1525	redundant	1517:1525	Conversely, downregulating the low-expressed TkCPTL2 showed no altered phenotype, suggesting its protein function is redundant in T. koksaghyz.
31342578	1	81	theme	considerable	170:181	arg1	amounts					183:189	considerable amounts	170:189	considerable amounts of high-molecular-weight rubber in its roots	170:234	The Russian dandelion Taraxacum koksaghyz synthesizes considerable amounts of high-molecular-weight rubber in its roots.
31342578	1	81	theme	considerable	170:181	arg1	rubber					216:221	high-molecular-weight rubber	194:221	high-molecular-weight rubber in its roots	194:234	The Russian dandelion Taraxacum koksaghyz synthesizes considerable amounts of high-molecular-weight rubber in its roots.
31342578	6	82	theme	abolished	935:943	arg1	synthesis					968:976	abolished poly(cis-1,4-isoprene) synthesis	935:976	abolished poly(cis-1,4-isoprene) synthesis	935:976	Knockdown of TkCPTL1, which is predominantly expressed in latex, led to abolished poly(cis-1,4-isoprene) synthesis but unaffected dolichol content, whereas levels of triterpenes and inulin were elevated in roots.
31141234	11	0	theme	unique	1430:1435	arg1	signatures					1467:1476	unique fecal microbial and metabolic signatures	1430:1476	unique fecal microbial and metabolic signatures in psoriatic patients	1430:1498	These data demonstrate unique fecal microbial and metabolic signatures in psoriatic patients.
31141234	2	1	with	patients	318:325	arg1	psoriasis					339:347	active psoriasis	332:347	active psoriasis	332:347	This work aimed to characterize the gut microbial signature of patients with active psoriasis as compared with age-, body mass index- and comorbidity-matched non-psoriatic controls and to correlate them with differential expression of metabolic pathways.
31141234	11	2	theme	fecal	1437:1441	arg1	signatures					1467:1476	unique fecal microbial and metabolic signatures	1430:1476	unique fecal microbial and metabolic signatures in psoriatic patients	1430:1498	These data demonstrate unique fecal microbial and metabolic signatures in psoriatic patients.
31141234	3	3	theme	16S	543:545	arg1	rRNA					547:550	16S rRNA	543:550	16S rRNA	543:550	Fecal samples were processed and 16S rRNA was sequenced.
31141234	10	4	theme	PICRUSt	1285:1291	arg1	analysis					1293:1300	PICRUSt analysis	1285:1300	PICRUSt analysis	1285:1300	PICRUSt analysis revealed increases in metabolic pathways related to lipopolysaccharide function in the psoriatic cohort.
31141234	7	5	dep	Firmicutes	819:828	arg1	phyla					849:853	phyla	849:853	phyla	849:853	Psoriatic patients had a significant increase in the Firmicutes and Actinobacteria phyla as compared with matched controls.
31141234	7	5	dep	Firmicutes	819:828	arg1	the					815:817	the	815:817	the	815:817	Psoriatic patients had a significant increase in the Firmicutes and Actinobacteria phyla as compared with matched controls.
31141234	4	6	used	used	579:582	arg2	PICRUSt					567:573	PICRUSt	567:573	PICRUSt	567:573	PICRUSt was used to perform an analysis of metabolic pathways.
31141234	7	7	contain	had	785:787	arg1	patients					776:783	Psoriatic patients	766:783	Psoriatic patients	766:783	Psoriatic patients had a significant increase in the Firmicutes and Actinobacteria phyla as compared with matched controls.
31141234	7	7	contain	had	785:787	arg2	increase					803:810	a significant increase	789:810	a significant increase in the Firmicutes and Actinobacteria phyla	789:853	Psoriatic patients had a significant increase in the Firmicutes and Actinobacteria phyla as compared with matched controls.
31141234	9	8	theme	false	1071:1075	arg1	<0.05					1093:1097	<0.05	1093:1097	<0.05	1093:1097	At the species level, the psoriatic patients showed significant increases in the relative proportions of (false discovery rate, <0.05) in Ruminoccocus gnavus, Dorea formicigenerans and Collinsella aerofaciens, while Prevotella copri and Parabacteroides distasonis were significantly decreased as compared with controls.
31141234	9	8	theme	false	1071:1075	arg1	rate					1087:1090	false discovery rate	1071:1090	false discovery rate	1071:1090	At the species level, the psoriatic patients showed significant increases in the relative proportions of (false discovery rate, <0.05) in Ruminoccocus gnavus, Dorea formicigenerans and Collinsella aerofaciens, while Prevotella copri and Parabacteroides distasonis were significantly decreased as compared with controls.
31141234	4	9	theme	pathways	620:627	arg1	analysis					598:605	an analysis	595:605	an analysis of metabolic pathways	595:627	PICRUSt was used to perform an analysis of metabolic pathways.
31141234	11	10	theme	psoriatic	1481:1489	arg1	patients					1491:1498	psoriatic patients	1481:1498	psoriatic patients	1481:1498	These data demonstrate unique fecal microbial and metabolic signatures in psoriatic patients.
31141234	2	11	theme	microbial	295:303	arg1	signature					305:313	the gut microbial signature	287:313	the gut microbial signature of patients with active psoriasis	287:347	This work aimed to characterize the gut microbial signature of patients with active psoriasis as compared with age-, body mass index- and comorbidity-matched non-psoriatic controls and to correlate them with differential expression of metabolic pathways.
31141234	7	12	theme	matched	872:878	arg1	controls					880:887	matched controls	872:887	matched controls	872:887	Psoriatic patients had a significant increase in the Firmicutes and Actinobacteria phyla as compared with matched controls.
31141234	4	13	theme	metabolic	610:618	arg1	pathways					620:627	metabolic pathways	610:627	metabolic pathways	610:627	PICRUSt was used to perform an analysis of metabolic pathways.
31141234	11	14	theme	metabolic	1457:1465	arg1	signatures					1467:1476	unique fecal microbial and metabolic signatures	1430:1476	unique fecal microbial and metabolic signatures in psoriatic patients	1430:1498	These data demonstrate unique fecal microbial and metabolic signatures in psoriatic patients.
31141234	9	15	theme	discovery	1077:1085	arg1	<0.05					1093:1097	<0.05	1093:1097	<0.05	1093:1097	At the species level, the psoriatic patients showed significant increases in the relative proportions of (false discovery rate, <0.05) in Ruminoccocus gnavus, Dorea formicigenerans and Collinsella aerofaciens, while Prevotella copri and Parabacteroides distasonis were significantly decreased as compared with controls.
31141234	9	15	theme	discovery	1077:1085	arg1	rate					1087:1090	false discovery rate	1071:1090	false discovery rate	1071:1090	At the species level, the psoriatic patients showed significant increases in the relative proportions of (false discovery rate, <0.05) in Ruminoccocus gnavus, Dorea formicigenerans and Collinsella aerofaciens, while Prevotella copri and Parabacteroides distasonis were significantly decreased as compared with controls.
31141234	7	16	from	increase	803:810	arg1	Actinobacteria					834:847	Actinobacteria	834:847	Actinobacteria	834:847	Psoriatic patients had a significant increase in the Firmicutes and Actinobacteria phyla as compared with matched controls.
31141234	7	16	from	increase	803:810	arg1	Firmicutes					819:828	Firmicutes	819:828	Firmicutes	819:828	Psoriatic patients had a significant increase in the Firmicutes and Actinobacteria phyla as compared with matched controls.
31141234	2	17	theme	gut	291:293	arg1	signature					305:313	the gut microbial signature	287:313	the gut microbial signature of patients with active psoriasis	287:347	This work aimed to characterize the gut microbial signature of patients with active psoriasis as compared with age-, body mass index- and comorbidity-matched non-psoriatic controls and to correlate them with differential expression of metabolic pathways.
31141234	6	18	theme	significant	704:714	arg1	difference					716:725	a significant difference	702:725	a significant difference in β-diversity between the two groups	702:763	There was a significant difference in β-diversity between the two groups.
31141234	2	19	theme	non-psoriatic	413:425	arg1	controls					427:434	comorbidity-matched non-psoriatic controls	393:434	comorbidity-matched non-psoriatic controls	393:434	This work aimed to characterize the gut microbial signature of patients with active psoriasis as compared with age-, body mass index- and comorbidity-matched non-psoriatic controls and to correlate them with differential expression of metabolic pathways.
31141234	9	20	theme	psoriatic	991:999	arg1	patients					1001:1008	the psoriatic patients	987:1008	the psoriatic patients	987:1008	At the species level, the psoriatic patients showed significant increases in the relative proportions of (false discovery rate, <0.05) in Ruminoccocus gnavus, Dorea formicigenerans and Collinsella aerofaciens, while Prevotella copri and Parabacteroides distasonis were significantly decreased as compared with controls.
31141234	0	21	contain	have	19:22	arg1	patients					10:17	Psoriatic patients	0:17	Psoriatic patients	0:17	Psoriatic patients have a distinct structural and functional fecal microbiota compared with controls.
31141234	0	21	contain	have	19:22	arg2	microbiota					67:76	a distinct structural and functional fecal microbiota	24:76	a distinct structural and functional fecal microbiota	24:76	Psoriatic patients have a distinct structural and functional fecal microbiota compared with controls.
31141234	0	22	theme	Psoriatic	0:8	arg1	patients					10:17	Psoriatic patients	0:17	Psoriatic patients	0:17	Psoriatic patients have a distinct structural and functional fecal microbiota compared with controls.
31141234	8	23	theme	genus	897:901	arg1	level					903:907	the genus level	893:907	the genus level	893:907	At the genus level, psoriatic patients had a unique bacterial composition.
31141234	2	24	theme	comorbidity-matched	393:411	arg1	controls					427:434	comorbidity-matched non-psoriatic controls	393:434	comorbidity-matched non-psoriatic controls	393:434	This work aimed to characterize the gut microbial signature of patients with active psoriasis as compared with age-, body mass index- and comorbidity-matched non-psoriatic controls and to correlate them with differential expression of metabolic pathways.
31141234	9	25	theme	species	972:978	arg1	level					980:984	the species level	968:984	the species level	968:984	At the species level, the psoriatic patients showed significant increases in the relative proportions of (false discovery rate, <0.05) in Ruminoccocus gnavus, Dorea formicigenerans and Collinsella aerofaciens, while Prevotella copri and Parabacteroides distasonis were significantly decreased as compared with controls.
31141234	2	26	theme	active	332:337	arg1	psoriasis					339:347	active psoriasis	332:347	active psoriasis	332:347	This work aimed to characterize the gut microbial signature of patients with active psoriasis as compared with age-, body mass index- and comorbidity-matched non-psoriatic controls and to correlate them with differential expression of metabolic pathways.
31141234	1	27	theme	several	180:186	arg1	diseases					217:224	several immune-mediated inflammatory diseases	180:224	several immune-mediated inflammatory diseases such as psoriatic arthritis	180:252	Alterations in the gut microbiome have been implicated in the pathogenesis of several immune-mediated inflammatory diseases such as psoriatic arthritis.
31141234	1	27	theme	several	180:186	arg1	arthritis					244:252	psoriatic arthritis	234:252	psoriatic arthritis	234:252	Alterations in the gut microbiome have been implicated in the pathogenesis of several immune-mediated inflammatory diseases such as psoriatic arthritis.
31141234	2	28	theme	differential	463:474	arg1	expression					476:485	differential expression	463:485	differential expression of metabolic pathways	463:507	This work aimed to characterize the gut microbial signature of patients with active psoriasis as compared with age-, body mass index- and comorbidity-matched non-psoriatic controls and to correlate them with differential expression of metabolic pathways.
31141234	1	29	theme	immune-mediated	188:202	arg1	diseases					217:224	several immune-mediated inflammatory diseases	180:224	several immune-mediated inflammatory diseases such as psoriatic arthritis	180:252	Alterations in the gut microbiome have been implicated in the pathogenesis of several immune-mediated inflammatory diseases such as psoriatic arthritis.
31141234	1	29	theme	immune-mediated	188:202	arg1	arthritis					244:252	psoriatic arthritis	234:252	psoriatic arthritis	234:252	Alterations in the gut microbiome have been implicated in the pathogenesis of several immune-mediated inflammatory diseases such as psoriatic arthritis.
31141234	2	30	theme	patients	318:325	arg1	signature					305:313	the gut microbial signature	287:313	the gut microbial signature of patients with active psoriasis	287:347	This work aimed to characterize the gut microbial signature of patients with active psoriasis as compared with age-, body mass index- and comorbidity-matched non-psoriatic controls and to correlate them with differential expression of metabolic pathways.
31141234	10	31	theme	lipopolysaccharide	1354:1371	arg1	function					1373:1380	lipopolysaccharide function	1354:1380	lipopolysaccharide function in the psoriatic cohort	1354:1404	PICRUSt analysis revealed increases in metabolic pathways related to lipopolysaccharide function in the psoriatic cohort.
31141234	1	32	theme	inflammatory	204:215	arg1	diseases					217:224	several immune-mediated inflammatory diseases	180:224	several immune-mediated inflammatory diseases such as psoriatic arthritis	180:252	Alterations in the gut microbiome have been implicated in the pathogenesis of several immune-mediated inflammatory diseases such as psoriatic arthritis.
31141234	1	32	theme	inflammatory	204:215	arg1	arthritis					244:252	psoriatic arthritis	234:252	psoriatic arthritis	234:252	Alterations in the gut microbiome have been implicated in the pathogenesis of several immune-mediated inflammatory diseases such as psoriatic arthritis.
31141234	6	33	from	difference	716:725	arg1	β-diversity					730:740	β-diversity	730:740	β-diversity	730:740	There was a significant difference in β-diversity between the two groups.
31141234	10	34	theme	related	1343:1349	arg1	pathways					1334:1341	metabolic pathways	1324:1341	metabolic pathways related to lipopolysaccharide function in the psoriatic cohort	1324:1404	PICRUSt analysis revealed increases in metabolic pathways related to lipopolysaccharide function in the psoriatic cohort.
31141234	1	35	theme	diseases	217:224	arg1	pathogenesis					164:175	the pathogenesis	160:175	the pathogenesis of several immune-mediated inflammatory diseases such as psoriatic arthritis	160:252	Alterations in the gut microbiome have been implicated in the pathogenesis of several immune-mediated inflammatory diseases such as psoriatic arthritis.
31141234	0	36	theme	structural	35:44	arg1	microbiota					67:76	a distinct structural and functional fecal microbiota	24:76	a distinct structural and functional fecal microbiota	24:76	Psoriatic patients have a distinct structural and functional fecal microbiota compared with controls.
31141234	10	37	theme	metabolic	1324:1332	arg1	pathways					1334:1341	metabolic pathways	1324:1341	metabolic pathways related to lipopolysaccharide function in the psoriatic cohort	1324:1404	PICRUSt analysis revealed increases in metabolic pathways related to lipopolysaccharide function in the psoriatic cohort.
31141234	11	38	from	signatures	1467:1476	arg1	patients					1491:1498	psoriatic patients	1481:1498	psoriatic patients	1481:1498	These data demonstrate unique fecal microbial and metabolic signatures in psoriatic patients.
31141234	8	39	theme	psoriatic	910:918	arg1	patients					920:927	psoriatic patients	910:927	psoriatic patients	910:927	At the genus level, psoriatic patients had a unique bacterial composition.
31141234	0	40	theme	distinct	26:33	arg1	microbiota					67:76	a distinct structural and functional fecal microbiota	24:76	a distinct structural and functional fecal microbiota	24:76	Psoriatic patients have a distinct structural and functional fecal microbiota compared with controls.
31141234	2	41	theme	pathways	500:507	arg1	expression					476:485	differential expression	463:485	differential expression of metabolic pathways	463:507	This work aimed to characterize the gut microbial signature of patients with active psoriasis as compared with age-, body mass index- and comorbidity-matched non-psoriatic controls and to correlate them with differential expression of metabolic pathways.
31141234	10	42	from	increases	1311:1319	arg1	pathways					1334:1341	metabolic pathways	1324:1341	metabolic pathways related to lipopolysaccharide function in the psoriatic cohort	1324:1404	PICRUSt analysis revealed increases in metabolic pathways related to lipopolysaccharide function in the psoriatic cohort.
31141234	2	43	theme	metabolic	490:498	arg1	pathways					500:507	metabolic pathways	490:507	metabolic pathways	490:507	This work aimed to characterize the gut microbial signature of patients with active psoriasis as compared with age-, body mass index- and comorbidity-matched non-psoriatic controls and to correlate them with differential expression of metabolic pathways.
31141234	7	44	theme	significant	791:801	arg1	increase					803:810	a significant increase	789:810	a significant increase in the Firmicutes and Actinobacteria phyla	789:853	Psoriatic patients had a significant increase in the Firmicutes and Actinobacteria phyla as compared with matched controls.
31141234	0	45	theme	fecal	61:65	arg1	microbiota					67:76	a distinct structural and functional fecal microbiota	24:76	a distinct structural and functional fecal microbiota	24:76	Psoriatic patients have a distinct structural and functional fecal microbiota compared with controls.
31141234	10	46	theme	psoriatic	1389:1397	arg1	cohort					1399:1404	the psoriatic cohort	1385:1404	the psoriatic cohort	1385:1404	PICRUSt analysis revealed increases in metabolic pathways related to lipopolysaccharide function in the psoriatic cohort.
31141234	9	47	theme	significant	1017:1027	arg1	increases					1029:1037	significant increases	1017:1037	significant increases in the relative proportions of (false discovery rate, <0.05)	1017:1098	At the species level, the psoriatic patients showed significant increases in the relative proportions of (false discovery rate, <0.05) in Ruminoccocus gnavus, Dorea formicigenerans and Collinsella aerofaciens, while Prevotella copri and Parabacteroides distasonis were significantly decreased as compared with controls.
31141234	8	48	contain	had	929:931	arg2	composition					952:962	a unique bacterial composition	933:962	a unique bacterial composition	933:962	At the genus level, psoriatic patients had a unique bacterial composition.
31141234	8	48	contain	had	929:931	arg1	patients					920:927	psoriatic patients	910:927	psoriatic patients	910:927	At the genus level, psoriatic patients had a unique bacterial composition.
31141234	8	49	theme	bacterial	942:950	arg1	composition					952:962	a unique bacterial composition	933:962	a unique bacterial composition	933:962	At the genus level, psoriatic patients had a unique bacterial composition.
31141234	1	50	theme	gut	121:123	arg1	microbiome					125:134	the gut microbiome	117:134	the gut microbiome	117:134	Alterations in the gut microbiome have been implicated in the pathogenesis of several immune-mediated inflammatory diseases such as psoriatic arthritis.
31141234	1	51	from	Alterations	102:112	arg1	microbiome					125:134	the gut microbiome	117:134	the gut microbiome	117:134	Alterations in the gut microbiome have been implicated in the pathogenesis of several immune-mediated inflammatory diseases such as psoriatic arthritis.
31141234	8	52	theme	unique	935:940	arg1	composition					952:962	a unique bacterial composition	933:962	a unique bacterial composition	933:962	At the genus level, psoriatic patients had a unique bacterial composition.
31141234	3	53	theme	Fecal	510:514	arg1	samples					516:522	Fecal samples	510:522	Fecal samples	510:522	Fecal samples were processed and 16S rRNA was sequenced.
31141234	9	54	from	increases	1029:1037	arg1	proportions					1055:1065	the relative proportions	1042:1065	the relative proportions of (false discovery rate, <0.05)	1042:1098	At the species level, the psoriatic patients showed significant increases in the relative proportions of (false discovery rate, <0.05) in Ruminoccocus gnavus, Dorea formicigenerans and Collinsella aerofaciens, while Prevotella copri and Parabacteroides distasonis were significantly decreased as compared with controls.
31141234	1	55	theme	psoriatic	234:242	arg1	arthritis					244:252	psoriatic arthritis	234:252	psoriatic arthritis	234:252	Alterations in the gut microbiome have been implicated in the pathogenesis of several immune-mediated inflammatory diseases such as psoriatic arthritis.
31141234	7	56	theme	Psoriatic	766:774	arg1	patients					776:783	Psoriatic patients	766:783	Psoriatic patients	766:783	Psoriatic patients had a significant increase in the Firmicutes and Actinobacteria phyla as compared with matched controls.
31141234	11	57	theme	microbial	1443:1451	arg1	signatures					1467:1476	unique fecal microbial and metabolic signatures	1430:1476	unique fecal microbial and metabolic signatures in psoriatic patients	1430:1498	These data demonstrate unique fecal microbial and metabolic signatures in psoriatic patients.
31141234	10	58	from	function	1373:1380	arg1	cohort					1399:1404	the psoriatic cohort	1385:1404	the psoriatic cohort	1385:1404	PICRUSt analysis revealed increases in metabolic pathways related to lipopolysaccharide function in the psoriatic cohort.
31141234	2	59	theme	mass	377:380	arg1	index-					382:387	body mass index-	372:387	body mass index-	372:387	This work aimed to characterize the gut microbial signature of patients with active psoriasis as compared with age-, body mass index- and comorbidity-matched non-psoriatic controls and to correlate them with differential expression of metabolic pathways.
31141234	9	60	theme	relative	1046:1053	arg1	proportions					1055:1065	the relative proportions	1042:1065	the relative proportions of (false discovery rate, <0.05)	1042:1098	At the species level, the psoriatic patients showed significant increases in the relative proportions of (false discovery rate, <0.05) in Ruminoccocus gnavus, Dorea formicigenerans and Collinsella aerofaciens, while Prevotella copri and Parabacteroides distasonis were significantly decreased as compared with controls.
31141234	0	61	theme	functional	50:59	arg1	microbiota					67:76	a distinct structural and functional fecal microbiota	24:76	a distinct structural and functional fecal microbiota	24:76	Psoriatic patients have a distinct structural and functional fecal microbiota compared with controls.
31141234	2	62	theme	body	372:375	arg1	index-					382:387	body mass index-	372:387	body mass index-	372:387	This work aimed to characterize the gut microbial signature of patients with active psoriasis as compared with age-, body mass index- and comorbidity-matched non-psoriatic controls and to correlate them with differential expression of metabolic pathways.
30429227	7	0	theme	gastrointestinal	1746:1761	arg1	tract					1763:1767	the gastrointestinal tract	1742:1767	the gastrointestinal tract	1742:1767	Recent studies suggested that consideration of the microbiota is unavoidable to understand inflammation and tumorigenesis in the gastrointestinal tract.
30429227	7	1	from	tumorigenesis	1725:1737	arg1	tract					1763:1767	the gastrointestinal tract	1742:1767	the gastrointestinal tract	1742:1767	Recent studies suggested that consideration of the microbiota is unavoidable to understand inflammation and tumorigenesis in the gastrointestinal tract.
30429227	6	2	theme	colitis-associated	1219:1236	arg1	animals					1242:1248	colitis-associated CRC animals	1219:1248	colitis-associated CRC animals	1219:1248	We show direct evidence that the inhibition of tumor formation provided by B. fragilis in colitis-associated CRC animals was dependent on the production of polysaccharide A (PSA) from B. fragilis and that Toll-like receptor 2 (TLR2) signaling was responsible for the protective function of B. fragilisIMPORTANCE The incidence of colorectal cancer (CRC) is rapidly growing worldwide, and there is therefore a greater emphasis on studies of the treatment or prevention of CRC pathogenesis.
30429227	1	3	theme	colorectal	222:231	arg1	CRC					241:243	CRC	241:243	CRC	241:243	Many patients with chronic inflammation of the gut, such as that observed in inflammatory bowel disease (IBD), develop colorectal cancer (CRC).
30429227	1	3	theme	colorectal	222:231	arg1	cancer					233:238	colorectal cancer	222:238	colorectal cancer (CRC)	222:244	Many patients with chronic inflammation of the gut, such as that observed in inflammatory bowel disease (IBD), develop colorectal cancer (CRC).
30429227	11	4	used	used	2287:2290	arg2	microorganism					2254:2266	the commensal microorganism	2240:2266	the commensal microorganism B. fragilis	2240:2278	Our results suggest that the commensal microorganism B. fragilis can be used to prevent inflammation-associated CRC development and may provide an effective therapeutic strategy for CRC.
30429227	8	5	theme	colitis-associated	1809:1826	arg1	CRC					1828:1830	colitis-associated CRC	1809:1830	colitis-associated CRC	1809:1830	We demonstrate, using a mouse model of colitis-associated CRC, that human commensal B. fragilis protects against colon tumorigenesis.
30429227	6	6	theme	cancer	1469:1474	arg1	incidence					1445:1453	B. fragilisIMPORTANCE The incidence	1419:1453	B. fragilisIMPORTANCE The incidence of colorectal cancer (CRC)	1419:1480	We show direct evidence that the inhibition of tumor formation provided by B. fragilis in colitis-associated CRC animals was dependent on the production of polysaccharide A (PSA) from B. fragilis and that Toll-like receptor 2 (TLR2) signaling was responsible for the protective function of B. fragilisIMPORTANCE The incidence of colorectal cancer (CRC) is rapidly growing worldwide, and there is therefore a greater emphasis on studies of the treatment or prevention of CRC pathogenesis.
30429227	5	7	theme	protective	806:815	arg1	role					817:820	a protective role	804:820	a protective role of B. fragilis treatment against colon tumorigenesis using an azoxymethane (AOM)/DSS-induced model of colitis-associated colon cancer in mice	804:962	Further, we reveal a protective role of B. fragilis treatment against colon tumorigenesis using an azoxymethane (AOM)/DSS-induced model of colitis-associated colon cancer in mice and demonstrate that the decreased tumorigenesis by B. fragilis administration is accompanied by inhibited expression of C-C chemokine receptor 5 (CCR5) in the gut.
30429227	6	8	theme	formation	1182:1190	arg1	dependent					1254:1262	dependent	1254:1262	dependent	1254:1262	We show direct evidence that the inhibition of tumor formation provided by B. fragilis in colitis-associated CRC animals was dependent on the production of polysaccharide A (PSA) from B. fragilis and that Toll-like receptor 2 (TLR2) signaling was responsible for the protective function of B. fragilisIMPORTANCE The incidence of colorectal cancer (CRC) is rapidly growing worldwide, and there is therefore a greater emphasis on studies of the treatment or prevention of CRC pathogenesis.
30429227	6	8	theme	formation	1182:1190	arg1	inhibition					1162:1171	the inhibition	1158:1171	the inhibition of tumor formation provided by B. fragilis in colitis-associated CRC animals	1158:1248	We show direct evidence that the inhibition of tumor formation provided by B. fragilis in colitis-associated CRC animals was dependent on the production of polysaccharide A (PSA) from B. fragilis and that Toll-like receptor 2 (TLR2) signaling was responsible for the protective function of B. fragilisIMPORTANCE The incidence of colorectal cancer (CRC) is rapidly growing worldwide, and there is therefore a greater emphasis on studies of the treatment or prevention of CRC pathogenesis.
30429227	3	9	theme	protective	541:550	arg1	role					552:555	a protective role	539:555	a protective role	539:555	The human commensal Bacteroides fragilis is best exemplified playing a protective role against the development of experimental colitis in several animal disease models.
30429227	11	10	theme	commensal	2244:2252	arg1	microorganism					2254:2266	the commensal microorganism	2240:2266	the commensal microorganism B. fragilis	2240:2278	Our results suggest that the commensal microorganism B. fragilis can be used to prevent inflammation-associated CRC development and may provide an effective therapeutic strategy for CRC.
30429227	8	11	theme	human	1838:1842	arg1	commensal					1844:1852	human commensal	1838:1852	human commensal B. fragilis	1838:1864	We demonstrate, using a mouse model of colitis-associated CRC, that human commensal B. fragilis protects against colon tumorigenesis.
30429227	6	12	theme	The	1441:1443	arg1	incidence					1445:1453	B. fragilisIMPORTANCE The incidence	1419:1453	B. fragilisIMPORTANCE The incidence of colorectal cancer (CRC)	1419:1480	We show direct evidence that the inhibition of tumor formation provided by B. fragilis in colitis-associated CRC animals was dependent on the production of polysaccharide A (PSA) from B. fragilis and that Toll-like receptor 2 (TLR2) signaling was responsible for the protective function of B. fragilisIMPORTANCE The incidence of colorectal cancer (CRC) is rapidly growing worldwide, and there is therefore a greater emphasis on studies of the treatment or prevention of CRC pathogenesis.
30429227	1	13	theme	inflammatory	180:191	arg1	IBD					208:210	IBD	208:210	IBD	208:210	Many patients with chronic inflammation of the gut, such as that observed in inflammatory bowel disease (IBD), develop colorectal cancer (CRC).
30429227	1	13	theme	inflammatory	180:191	arg1	disease					199:205	inflammatory bowel disease	180:205	inflammatory bowel disease (IBD)	180:211	Many patients with chronic inflammation of the gut, such as that observed in inflammatory bowel disease (IBD), develop colorectal cancer (CRC).
30429227	6	14	dep	show	1132:1135	arg1	growing					1493:1499	growing	1493:1499	show direct evidence that the inhibition of tumor formation provided by B. fragilis in colitis-associated CRC animals was dependent on the production of polysaccharide A (PSA) from B. fragilis and that Toll-like receptor 2 (TLR2) signaling was responsible for the protective function of B. fragilisIMPORTANCE The incidence of colorectal cancer (CRC) is rapidly growing worldwide	1132:1509	We show direct evidence that the inhibition of tumor formation provided by B. fragilis in colitis-associated CRC animals was dependent on the production of polysaccharide A (PSA) from B. fragilis and that Toll-like receptor 2 (TLR2) signaling was responsible for the protective function of B. fragilisIMPORTANCE The incidence of colorectal cancer (CRC) is rapidly growing worldwide, and there is therefore a greater emphasis on studies of the treatment or prevention of CRC pathogenesis.
30429227	6	15	theme	pathogenesis	1603:1614	arg1	prevention					1585:1594	prevention	1585:1594	prevention	1585:1594	We show direct evidence that the inhibition of tumor formation provided by B. fragilis in colitis-associated CRC animals was dependent on the production of polysaccharide A (PSA) from B. fragilis and that Toll-like receptor 2 (TLR2) signaling was responsible for the protective function of B. fragilisIMPORTANCE The incidence of colorectal cancer (CRC) is rapidly growing worldwide, and there is therefore a greater emphasis on studies of the treatment or prevention of CRC pathogenesis.
30429227	6	15	theme	pathogenesis	1603:1614	arg1	treatment					1572:1580	the treatment	1568:1580	the treatment	1568:1580	We show direct evidence that the inhibition of tumor formation provided by B. fragilis in colitis-associated CRC animals was dependent on the production of polysaccharide A (PSA) from B. fragilis and that Toll-like receptor 2 (TLR2) signaling was responsible for the protective function of B. fragilisIMPORTANCE The incidence of colorectal cancer (CRC) is rapidly growing worldwide, and there is therefore a greater emphasis on studies of the treatment or prevention of CRC pathogenesis.
30429227	6	16	theme	tumor	1176:1180	arg1	formation					1182:1190	tumor formation	1176:1190	tumor formation provided by B. fragilis in colitis-associated CRC animals	1176:1248	We show direct evidence that the inhibition of tumor formation provided by B. fragilis in colitis-associated CRC animals was dependent on the production of polysaccharide A (PSA) from B. fragilis and that Toll-like receptor 2 (TLR2) signaling was responsible for the protective function of B. fragilisIMPORTANCE The incidence of colorectal cancer (CRC) is rapidly growing worldwide, and there is therefore a greater emphasis on studies of the treatment or prevention of CRC pathogenesis.
30429227	8	17	theme	B.	1854:1855	arg1	commensal					1844:1852	human commensal	1838:1852	human commensal B. fragilis	1838:1864	We demonstrate, using a mouse model of colitis-associated CRC, that human commensal B. fragilis protects against colon tumorigenesis.
30429227	1	18	located	observed	168:175	arg2	that					163:166	that	163:166	that	163:166	Many patients with chronic inflammation of the gut, such as that observed in inflammatory bowel disease (IBD), develop colorectal cancer (CRC).
30429227	1	18	located	observed	168:175	arg1	IBD					208:210	IBD	208:210	IBD	208:210	Many patients with chronic inflammation of the gut, such as that observed in inflammatory bowel disease (IBD), develop colorectal cancer (CRC).
30429227	1	18	located	observed	168:175	arg1	disease					199:205	inflammatory bowel disease	180:205	inflammatory bowel disease (IBD)	180:211	Many patients with chronic inflammation of the gut, such as that observed in inflammatory bowel disease (IBD), develop colorectal cancer (CRC).
30429227	5	19	theme	decreased	989:997	arg1	tumorigenesis					999:1011	the decreased tumorigenesis	985:1011	the decreased tumorigenesis by B. fragilis administration	985:1041	Further, we reveal a protective role of B. fragilis treatment against colon tumorigenesis using an azoxymethane (AOM)/DSS-induced model of colitis-associated colon cancer in mice and demonstrate that the decreased tumorigenesis by B. fragilis administration is accompanied by inhibited expression of C-C chemokine receptor 5 (CCR5) in the gut.
30429227	8	20	theme	colon	1883:1887	arg1	tumorigenesis					1889:1901	colon tumorigenesis	1883:1901	colon tumorigenesis	1883:1901	We demonstrate, using a mouse model of colitis-associated CRC, that human commensal B. fragilis protects against colon tumorigenesis.
30429227	6	21	theme	direct	1137:1142	arg1	evidence					1144:1151	direct evidence that the inhibition of tumor formation provided by B. fragilis in colitis-associated CRC animals was dependent on the production of polysaccharide A (PSA) from B. fragilis and that Toll-like receptor 2 (TLR2) signaling was responsible for the protective function of B. fragilisIMPORTANCE The incidence of colorectal cancer (CRC)	1137:1480	direct evidence that the inhibition of tumor formation provided by B. fragilis in colitis-associated CRC animals was dependent on the production of polysaccharide A (PSA) from B. fragilis and that Toll-like receptor 2 (TLR2) signaling was responsible for the protective function of B. fragilisIMPORTANCE The incidence of colorectal cancer (CRC)	1137:1480	We show direct evidence that the inhibition of tumor formation provided by B. fragilis in colitis-associated CRC animals was dependent on the production of polysaccharide A (PSA) from B. fragilis and that Toll-like receptor 2 (TLR2) signaling was responsible for the protective function of B. fragilisIMPORTANCE The incidence of colorectal cancer (CRC) is rapidly growing worldwide, and there is therefore a greater emphasis on studies of the treatment or prevention of CRC pathogenesis.
30429227	4	22	dep	B.	751:752	arg1	fragilis					754:761	fragilis	754:761	fragilis	754:761	In this study, we found that gut inflammation caused by dextran sulfate sodium (DSS) treatment was inhibited by B. fragilis colonization in mice.
30429227	0	23	theme	Murine	49:54	arg1	Model					56:60	a Murine Model	47:60	a Murine Model of Colitis-Associated Colorectal Cancer	47:100	The Protective Role of Bacteroides fragilis in a Murine Model of Colitis-Associated Colorectal Cancer.
30429227	5	24	theme	C-C	1085:1087	arg1	receptor					1099:1106	C-C chemokine receptor 5	1085:1108	C-C chemokine receptor 5 (CCR5)	1085:1115	Further, we reveal a protective role of B. fragilis treatment against colon tumorigenesis using an azoxymethane (AOM)/DSS-induced model of colitis-associated colon cancer in mice and demonstrate that the decreased tumorigenesis by B. fragilis administration is accompanied by inhibited expression of C-C chemokine receptor 5 (CCR5) in the gut.
30429227	5	24	theme	C-C	1085:1087	arg1	CCR5					1111:1114	CCR5	1111:1114	CCR5	1111:1114	Further, we reveal a protective role of B. fragilis treatment against colon tumorigenesis using an azoxymethane (AOM)/DSS-induced model of colitis-associated colon cancer in mice and demonstrate that the decreased tumorigenesis by B. fragilis administration is accompanied by inhibited expression of C-C chemokine receptor 5 (CCR5) in the gut.
30429227	3	25	theme	experimental	584:595	arg1	colitis					597:603	experimental colitis	584:603	experimental colitis	584:603	The human commensal Bacteroides fragilis is best exemplified playing a protective role against the development of experimental colitis in several animal disease models.
30429227	0	26	from	Role	15:18	arg1	Model					56:60	a Murine Model	47:60	a Murine Model of Colitis-Associated Colorectal Cancer	47:100	The Protective Role of Bacteroides fragilis in a Murine Model of Colitis-Associated Colorectal Cancer.
30429227	5	27	theme	receptor	1099:1106	arg1	expression					1071:1080	inhibited expression	1061:1080	inhibited expression of C-C chemokine receptor 5 (CCR5) in the gut	1061:1126	Further, we reveal a protective role of B. fragilis treatment against colon tumorigenesis using an azoxymethane (AOM)/DSS-induced model of colitis-associated colon cancer in mice and demonstrate that the decreased tumorigenesis by B. fragilis administration is accompanied by inhibited expression of C-C chemokine receptor 5 (CCR5) in the gut.
30429227	7	28	theme	Recent	1617:1622	arg1	studies					1624:1630	Recent studies	1617:1630	Recent studies	1617:1630	Recent studies suggested that consideration of the microbiota is unavoidable to understand inflammation and tumorigenesis in the gastrointestinal tract.
30429227	9	29	theme	expression	2005:2014	arg1	inhibition					1991:2000	inhibition	1991:2000	inhibition of expression of the chemokine receptor CCR5 in the colon	1991:2058	The protective role against tumor formation provided by B. fragilis is associated with inhibition of expression of the chemokine receptor CCR5 in the colon.
30429227	2	30	from	inflammation	403:414	arg1	diseases					425:432	these diseases	419:432	these diseases	419:432	Recent studies have reported that the development of IBD and CRC partly results from an imbalanced composition of intestinal microbiota and that intestinal inflammation in these diseases can be modulated by the microbiota.
30429227	0	31	theme	Colorectal	84:93	arg1	Cancer					95:100	Colitis-Associated Colorectal Cancer	65:100	Colitis-Associated Colorectal Cancer	65:100	The Protective Role of Bacteroides fragilis in a Murine Model of Colitis-Associated Colorectal Cancer.
30429227	10	32	theme	polysaccharide	2152:2165	arg1	production					2169:2178	polysaccharide A production	2152:2178	polysaccharide A production	2152:2178	The molecular mechanism for protection against CRC provided by B. fragilis is dependent on polysaccharide A production and is mediated by TLR2 signaling.
30429227	6	33	theme	A	1300:1300	arg1	production					1271:1280	the production	1267:1280	the production of polysaccharide A (PSA) from B. fragilis	1267:1323	We show direct evidence that the inhibition of tumor formation provided by B. fragilis in colitis-associated CRC animals was dependent on the production of polysaccharide A (PSA) from B. fragilis and that Toll-like receptor 2 (TLR2) signaling was responsible for the protective function of B. fragilisIMPORTANCE The incidence of colorectal cancer (CRC) is rapidly growing worldwide, and there is therefore a greater emphasis on studies of the treatment or prevention of CRC pathogenesis.
30429227	6	34	theme	greater	1537:1543	arg1	emphasis					1545:1552	a greater emphasis	1535:1552	a greater emphasis on studies of the treatment or prevention of CRC pathogenesis	1535:1614	We show direct evidence that the inhibition of tumor formation provided by B. fragilis in colitis-associated CRC animals was dependent on the production of polysaccharide A (PSA) from B. fragilis and that Toll-like receptor 2 (TLR2) signaling was responsible for the protective function of B. fragilisIMPORTANCE The incidence of colorectal cancer (CRC) is rapidly growing worldwide, and there is therefore a greater emphasis on studies of the treatment or prevention of CRC pathogenesis.
30429227	5	35	theme	colitis-associated	924:941	arg1	cancer					949:954	colitis-associated colon cancer	924:954	colitis-associated colon cancer in mice	924:962	Further, we reveal a protective role of B. fragilis treatment against colon tumorigenesis using an azoxymethane (AOM)/DSS-induced model of colitis-associated colon cancer in mice and demonstrate that the decreased tumorigenesis by B. fragilis administration is accompanied by inhibited expression of C-C chemokine receptor 5 (CCR5) in the gut.
30429227	11	36	theme	effective	2362:2370	arg1	strategy					2384:2391	an effective therapeutic strategy	2359:2391	an effective therapeutic strategy for CRC	2359:2399	Our results suggest that the commensal microorganism B. fragilis can be used to prevent inflammation-associated CRC development and may provide an effective therapeutic strategy for CRC.
30429227	4	37	theme	sulfate	703:709	arg1	sodium					711:716	dextran sulfate sodium	695:716	dextran sulfate sodium (DSS) treatment	695:732	In this study, we found that gut inflammation caused by dextran sulfate sodium (DSS) treatment was inhibited by B. fragilis colonization in mice.
30429227	4	37	theme	sulfate	703:709	arg1	DSS					719:721	DSS	719:721	DSS	719:721	In this study, we found that gut inflammation caused by dextran sulfate sodium (DSS) treatment was inhibited by B. fragilis colonization in mice.
30429227	2	38	theme	intestinal	392:401	arg1	inflammation					403:414	intestinal inflammation	392:414	intestinal inflammation in these diseases	392:432	Recent studies have reported that the development of IBD and CRC partly results from an imbalanced composition of intestinal microbiota and that intestinal inflammation in these diseases can be modulated by the microbiota.
30429227	5	39	dep	B.	825:826	arg1	fragilis					828:835	fragilis	828:835	fragilis	828:835	Further, we reveal a protective role of B. fragilis treatment against colon tumorigenesis using an azoxymethane (AOM)/DSS-induced model of colitis-associated colon cancer in mice and demonstrate that the decreased tumorigenesis by B. fragilis administration is accompanied by inhibited expression of C-C chemokine receptor 5 (CCR5) in the gut.
30429227	9	40	theme	tumor	1932:1936	arg1	formation					1938:1946	tumor formation	1932:1946	tumor formation provided by B. fragilis	1932:1970	The protective role against tumor formation provided by B. fragilis is associated with inhibition of expression of the chemokine receptor CCR5 in the colon.
30429227	11	41	theme	therapeutic	2372:2382	arg1	strategy					2384:2391	an effective therapeutic strategy	2359:2391	an effective therapeutic strategy for CRC	2359:2399	Our results suggest that the commensal microorganism B. fragilis can be used to prevent inflammation-associated CRC development and may provide an effective therapeutic strategy for CRC.
30429227	1	42	theme	Many	103:106	arg1	that					163:166	that	163:166	that	163:166	Many patients with chronic inflammation of the gut, such as that observed in inflammatory bowel disease (IBD), develop colorectal cancer (CRC).
30429227	1	42	theme	Many	103:106	arg1	patients					108:115	Many patients	103:115	Many patients	103:115	Many patients with chronic inflammation of the gut, such as that observed in inflammatory bowel disease (IBD), develop colorectal cancer (CRC).
30429227	3	43	from	development	569:579	arg1	models					631:636	several animal disease models	608:636	several animal disease models	608:636	The human commensal Bacteroides fragilis is best exemplified playing a protective role against the development of experimental colitis in several animal disease models.
30429227	2	44	theme	intestinal	361:370	arg1	microbiota					372:381	intestinal microbiota	361:381	intestinal microbiota	361:381	Recent studies have reported that the development of IBD and CRC partly results from an imbalanced composition of intestinal microbiota and that intestinal inflammation in these diseases can be modulated by the microbiota.
30429227	6	45	theme	receptor	1344:1351	arg1	signaling					1362:1370	Toll-like receptor 2 (TLR2) signaling	1334:1370	Toll-like receptor 2 (TLR2) signaling	1334:1370	We show direct evidence that the inhibition of tumor formation provided by B. fragilis in colitis-associated CRC animals was dependent on the production of polysaccharide A (PSA) from B. fragilis and that Toll-like receptor 2 (TLR2) signaling was responsible for the protective function of B. fragilisIMPORTANCE The incidence of colorectal cancer (CRC) is rapidly growing worldwide, and there is therefore a greater emphasis on studies of the treatment or prevention of CRC pathogenesis.
30429227	0	46	theme	Bacteroides	23:33	arg1	fragilis					35:42	Bacteroides fragilis	23:42	Bacteroides fragilis	23:42	The Protective Role of Bacteroides fragilis in a Murine Model of Colitis-Associated Colorectal Cancer.
30429227	3	47	theme	animal	616:621	arg1	models					631:636	several animal disease models	608:636	several animal disease models	608:636	The human commensal Bacteroides fragilis is best exemplified playing a protective role against the development of experimental colitis in several animal disease models.
30429227	2	48	theme	IBD	300:302	arg1	development					285:295	the development	281:295	the development of IBD and CRC	281:310	Recent studies have reported that the development of IBD and CRC partly results from an imbalanced composition of intestinal microbiota and that intestinal inflammation in these diseases can be modulated by the microbiota.
30429227	9	49	theme	CCR5	2042:2045	arg1	expression					2005:2014	expression	2005:2014	expression of the chemokine receptor CCR5 in the colon	2005:2058	The protective role against tumor formation provided by B. fragilis is associated with inhibition of expression of the chemokine receptor CCR5 in the colon.
30429227	5	50	theme	cancer	949:954	arg1	model					915:919	an azoxymethane (AOM)/DSS-induced model	881:919	an azoxymethane (AOM)/DSS-induced model of colitis-associated colon cancer in mice	881:962	Further, we reveal a protective role of B. fragilis treatment against colon tumorigenesis using an azoxymethane (AOM)/DSS-induced model of colitis-associated colon cancer in mice and demonstrate that the decreased tumorigenesis by B. fragilis administration is accompanied by inhibited expression of C-C chemokine receptor 5 (CCR5) in the gut.
30429227	3	51	theme	human	474:478	arg1	fragilis					502:509	The human commensal Bacteroides fragilis	470:509	The human commensal Bacteroides fragilis	470:509	The human commensal Bacteroides fragilis is best exemplified playing a protective role against the development of experimental colitis in several animal disease models.
30429227	6	52	from	emphasis	1545:1552	arg1	studies					1557:1563	studies	1557:1563	studies of the treatment or prevention of CRC pathogenesis	1557:1614	We show direct evidence that the inhibition of tumor formation provided by B. fragilis in colitis-associated CRC animals was dependent on the production of polysaccharide A (PSA) from B. fragilis and that Toll-like receptor 2 (TLR2) signaling was responsible for the protective function of B. fragilisIMPORTANCE The incidence of colorectal cancer (CRC) is rapidly growing worldwide, and there is therefore a greater emphasis on studies of the treatment or prevention of CRC pathogenesis.
30429227	9	53	from	colon	2054:2058	arg1	inhibition					1991:2000	inhibition	1991:2000	inhibition of expression of the chemokine receptor CCR5 in the colon	1991:2058	The protective role against tumor formation provided by B. fragilis is associated with inhibition of expression of the chemokine receptor CCR5 in the colon.
30429227	8	54	theme	mouse	1794:1798	arg1	model					1800:1804	a mouse model	1792:1804	a mouse model of colitis-associated CRC	1792:1830	We demonstrate, using a mouse model of colitis-associated CRC, that human commensal B. fragilis protects against colon tumorigenesis.
30429227	6	55	theme	CRC	1238:1240	arg1	animals					1242:1248	colitis-associated CRC animals	1219:1248	colitis-associated CRC animals	1219:1248	We show direct evidence that the inhibition of tumor formation provided by B. fragilis in colitis-associated CRC animals was dependent on the production of polysaccharide A (PSA) from B. fragilis and that Toll-like receptor 2 (TLR2) signaling was responsible for the protective function of B. fragilisIMPORTANCE The incidence of colorectal cancer (CRC) is rapidly growing worldwide, and there is therefore a greater emphasis on studies of the treatment or prevention of CRC pathogenesis.
30429227	2	56	theme	CRC	308:310	arg1	development					285:295	the development	281:295	the development of IBD and CRC	281:310	Recent studies have reported that the development of IBD and CRC partly results from an imbalanced composition of intestinal microbiota and that intestinal inflammation in these diseases can be modulated by the microbiota.
30429227	9	57	theme	chemokine	2023:2031	arg1	CCR5					2042:2045	the chemokine receptor CCR5	2019:2045	the chemokine receptor CCR5	2019:2045	The protective role against tumor formation provided by B. fragilis is associated with inhibition of expression of the chemokine receptor CCR5 in the colon.
30429227	5	58	dep	B.	1016:1017	arg1	fragilis					1019:1026	fragilis	1019:1026	fragilis	1019:1026	Further, we reveal a protective role of B. fragilis treatment against colon tumorigenesis using an azoxymethane (AOM)/DSS-induced model of colitis-associated colon cancer in mice and demonstrate that the decreased tumorigenesis by B. fragilis administration is accompanied by inhibited expression of C-C chemokine receptor 5 (CCR5) in the gut.
30429227	11	59	dep	B.	2268:2269	arg1	fragilis					2271:2278	fragilis	2271:2278	fragilis	2271:2278	Our results suggest that the commensal microorganism B. fragilis can be used to prevent inflammation-associated CRC development and may provide an effective therapeutic strategy for CRC.
30429227	2	60	theme	Recent	247:252	arg1	studies					254:260	Recent studies	247:260	Recent studies	247:260	Recent studies have reported that the development of IBD and CRC partly results from an imbalanced composition of intestinal microbiota and that intestinal inflammation in these diseases can be modulated by the microbiota.
30429227	5	61	from	model	915:919	arg1	mice					959:962	mice	959:962	mice	959:962	Further, we reveal a protective role of B. fragilis treatment against colon tumorigenesis using an azoxymethane (AOM)/DSS-induced model of colitis-associated colon cancer in mice and demonstrate that the decreased tumorigenesis by B. fragilis administration is accompanied by inhibited expression of C-C chemokine receptor 5 (CCR5) in the gut.
30429227	5	62	theme	treatment	837:845	arg1	role					817:820	a protective role	804:820	a protective role of B. fragilis treatment against colon tumorigenesis using an azoxymethane (AOM)/DSS-induced model of colitis-associated colon cancer in mice	804:962	Further, we reveal a protective role of B. fragilis treatment against colon tumorigenesis using an azoxymethane (AOM)/DSS-induced model of colitis-associated colon cancer in mice and demonstrate that the decreased tumorigenesis by B. fragilis administration is accompanied by inhibited expression of C-C chemokine receptor 5 (CCR5) in the gut.
30429227	10	63	theme	molecular	2065:2073	arg1	dependent					2139:2147	dependent	2139:2147	dependent	2139:2147	The molecular mechanism for protection against CRC provided by B. fragilis is dependent on polysaccharide A production and is mediated by TLR2 signaling.
30429227	10	63	theme	molecular	2065:2073	arg1	mechanism					2075:2083	The molecular mechanism	2061:2083	The molecular mechanism for protection against CRC provided by B. fragilis	2061:2134	The molecular mechanism for protection against CRC provided by B. fragilis is dependent on polysaccharide A production and is mediated by TLR2 signaling.
30429227	6	64	theme	colorectal	1458:1467	arg1	cancer					1469:1474	colorectal cancer	1458:1474	colorectal cancer (CRC)	1458:1480	We show direct evidence that the inhibition of tumor formation provided by B. fragilis in colitis-associated CRC animals was dependent on the production of polysaccharide A (PSA) from B. fragilis and that Toll-like receptor 2 (TLR2) signaling was responsible for the protective function of B. fragilisIMPORTANCE The incidence of colorectal cancer (CRC) is rapidly growing worldwide, and there is therefore a greater emphasis on studies of the treatment or prevention of CRC pathogenesis.
30429227	6	64	theme	colorectal	1458:1467	arg1	CRC					1477:1479	CRC	1477:1479	CRC	1477:1479	We show direct evidence that the inhibition of tumor formation provided by B. fragilis in colitis-associated CRC animals was dependent on the production of polysaccharide A (PSA) from B. fragilis and that Toll-like receptor 2 (TLR2) signaling was responsible for the protective function of B. fragilisIMPORTANCE The incidence of colorectal cancer (CRC) is rapidly growing worldwide, and there is therefore a greater emphasis on studies of the treatment or prevention of CRC pathogenesis.
30429227	8	65	theme	CRC	1828:1830	arg1	model					1800:1804	a mouse model	1792:1804	a mouse model of colitis-associated CRC	1792:1830	We demonstrate, using a mouse model of colitis-associated CRC, that human commensal B. fragilis protects against colon tumorigenesis.
30429227	3	66	theme	Bacteroides	490:500	arg1	fragilis					502:509	The human commensal Bacteroides fragilis	470:509	The human commensal Bacteroides fragilis	470:509	The human commensal Bacteroides fragilis is best exemplified playing a protective role against the development of experimental colitis in several animal disease models.
30429227	5	67	from	mice	959:962	arg1	model					915:919	an azoxymethane (AOM)/DSS-induced model	881:919	an azoxymethane (AOM)/DSS-induced model of colitis-associated colon cancer in mice	881:962	Further, we reveal a protective role of B. fragilis treatment against colon tumorigenesis using an azoxymethane (AOM)/DSS-induced model of colitis-associated colon cancer in mice and demonstrate that the decreased tumorigenesis by B. fragilis administration is accompanied by inhibited expression of C-C chemokine receptor 5 (CCR5) in the gut.
30429227	5	68	from	cancer	949:954	arg1	mice					959:962	mice	959:962	mice	959:962	Further, we reveal a protective role of B. fragilis treatment against colon tumorigenesis using an azoxymethane (AOM)/DSS-induced model of colitis-associated colon cancer in mice and demonstrate that the decreased tumorigenesis by B. fragilis administration is accompanied by inhibited expression of C-C chemokine receptor 5 (CCR5) in the gut.
30429227	6	69	theme	polysaccharide	1285:1298	arg1	PSA					1303:1305	PSA	1303:1305	PSA	1303:1305	We show direct evidence that the inhibition of tumor formation provided by B. fragilis in colitis-associated CRC animals was dependent on the production of polysaccharide A (PSA) from B. fragilis and that Toll-like receptor 2 (TLR2) signaling was responsible for the protective function of B. fragilisIMPORTANCE The incidence of colorectal cancer (CRC) is rapidly growing worldwide, and there is therefore a greater emphasis on studies of the treatment or prevention of CRC pathogenesis.
30429227	6	69	theme	polysaccharide	1285:1298	arg1	A					1300:1300	polysaccharide A	1285:1300	polysaccharide A (PSA)	1285:1306	We show direct evidence that the inhibition of tumor formation provided by B. fragilis in colitis-associated CRC animals was dependent on the production of polysaccharide A (PSA) from B. fragilis and that Toll-like receptor 2 (TLR2) signaling was responsible for the protective function of B. fragilisIMPORTANCE The incidence of colorectal cancer (CRC) is rapidly growing worldwide, and there is therefore a greater emphasis on studies of the treatment or prevention of CRC pathogenesis.
30429227	6	70	theme	incidence	1445:1453	arg1	function					1407:1414	the protective function	1392:1414	the protective function of B. fragilisIMPORTANCE The incidence of colorectal cancer (CRC)	1392:1480	We show direct evidence that the inhibition of tumor formation provided by B. fragilis in colitis-associated CRC animals was dependent on the production of polysaccharide A (PSA) from B. fragilis and that Toll-like receptor 2 (TLR2) signaling was responsible for the protective function of B. fragilisIMPORTANCE The incidence of colorectal cancer (CRC) is rapidly growing worldwide, and there is therefore a greater emphasis on studies of the treatment or prevention of CRC pathogenesis.
30429227	6	71	theme	fragilisIMPORTANCE	1422:1439	arg1	incidence					1445:1453	B. fragilisIMPORTANCE The incidence	1419:1453	B. fragilisIMPORTANCE The incidence of colorectal cancer (CRC)	1419:1480	We show direct evidence that the inhibition of tumor formation provided by B. fragilis in colitis-associated CRC animals was dependent on the production of polysaccharide A (PSA) from B. fragilis and that Toll-like receptor 2 (TLR2) signaling was responsible for the protective function of B. fragilisIMPORTANCE The incidence of colorectal cancer (CRC) is rapidly growing worldwide, and there is therefore a greater emphasis on studies of the treatment or prevention of CRC pathogenesis.
30429227	1	72	theme	bowel	193:197	arg1	IBD					208:210	IBD	208:210	IBD	208:210	Many patients with chronic inflammation of the gut, such as that observed in inflammatory bowel disease (IBD), develop colorectal cancer (CRC).
30429227	1	72	theme	bowel	193:197	arg1	disease					199:205	inflammatory bowel disease	180:205	inflammatory bowel disease (IBD)	180:211	Many patients with chronic inflammation of the gut, such as that observed in inflammatory bowel disease (IBD), develop colorectal cancer (CRC).
30429227	6	73	theme	CRC	1599:1601	arg1	pathogenesis					1603:1614	CRC pathogenesis	1599:1614	CRC pathogenesis	1599:1614	We show direct evidence that the inhibition of tumor formation provided by B. fragilis in colitis-associated CRC animals was dependent on the production of polysaccharide A (PSA) from B. fragilis and that Toll-like receptor 2 (TLR2) signaling was responsible for the protective function of B. fragilisIMPORTANCE The incidence of colorectal cancer (CRC) is rapidly growing worldwide, and there is therefore a greater emphasis on studies of the treatment or prevention of CRC pathogenesis.
30429227	5	74	theme	colon	855:859	arg1	tumorigenesis					861:873	colon tumorigenesis	855:873	colon tumorigenesis using an azoxymethane (AOM)/DSS-induced model of colitis-associated colon cancer in mice	855:962	Further, we reveal a protective role of B. fragilis treatment against colon tumorigenesis using an azoxymethane (AOM)/DSS-induced model of colitis-associated colon cancer in mice and demonstrate that the decreased tumorigenesis by B. fragilis administration is accompanied by inhibited expression of C-C chemokine receptor 5 (CCR5) in the gut.
30429227	6	75	theme	prevention	1585:1594	arg1	studies					1557:1563	studies	1557:1563	studies of the treatment or prevention of CRC pathogenesis	1557:1614	We show direct evidence that the inhibition of tumor formation provided by B. fragilis in colitis-associated CRC animals was dependent on the production of polysaccharide A (PSA) from B. fragilis and that Toll-like receptor 2 (TLR2) signaling was responsible for the protective function of B. fragilisIMPORTANCE The incidence of colorectal cancer (CRC) is rapidly growing worldwide, and there is therefore a greater emphasis on studies of the treatment or prevention of CRC pathogenesis.
30429227	9	76	theme	protective	1908:1917	arg1	role					1919:1922	The protective role	1904:1922	The protective role against tumor formation provided by B. fragilis	1904:1970	The protective role against tumor formation provided by B. fragilis is associated with inhibition of expression of the chemokine receptor CCR5 in the colon.
30429227	4	77	theme	gut	668:670	arg1	inflammation					672:683	gut inflammation	668:683	gut inflammation caused by dextran sulfate sodium (DSS) treatment	668:732	In this study, we found that gut inflammation caused by dextran sulfate sodium (DSS) treatment was inhibited by B. fragilis colonization in mice.
30429227	3	78	theme	colitis	597:603	arg1	development					569:579	the development	565:579	the development of experimental colitis in several animal disease models	565:636	The human commensal Bacteroides fragilis is best exemplified playing a protective role against the development of experimental colitis in several animal disease models.
30429227	4	79	from	colonization	763:774	arg1	mice					779:782	mice	779:782	mice	779:782	In this study, we found that gut inflammation caused by dextran sulfate sodium (DSS) treatment was inhibited by B. fragilis colonization in mice.
30429227	1	80	theme	chronic	122:128	arg1	inflammation					130:141	chronic inflammation	122:141	chronic inflammation of the gut	122:152	Many patients with chronic inflammation of the gut, such as that observed in inflammatory bowel disease (IBD), develop colorectal cancer (CRC).
30429227	7	81	theme	microbiota	1668:1677	arg1	consideration					1647:1659	consideration	1647:1659	consideration of the microbiota	1647:1677	Recent studies suggested that consideration of the microbiota is unavoidable to understand inflammation and tumorigenesis in the gastrointestinal tract.
30429227	6	82	theme	treatment	1572:1580	arg1	studies					1557:1563	studies	1557:1563	studies of the treatment or prevention of CRC pathogenesis	1557:1614	We show direct evidence that the inhibition of tumor formation provided by B. fragilis in colitis-associated CRC animals was dependent on the production of polysaccharide A (PSA) from B. fragilis and that Toll-like receptor 2 (TLR2) signaling was responsible for the protective function of B. fragilisIMPORTANCE The incidence of colorectal cancer (CRC) is rapidly growing worldwide, and there is therefore a greater emphasis on studies of the treatment or prevention of CRC pathogenesis.
30429227	5	83	theme	B.	1016:1017	arg1	administration					1028:1041	B. fragilis administration	1016:1041	B. fragilis administration	1016:1041	Further, we reveal a protective role of B. fragilis treatment against colon tumorigenesis using an azoxymethane (AOM)/DSS-induced model of colitis-associated colon cancer in mice and demonstrate that the decreased tumorigenesis by B. fragilis administration is accompanied by inhibited expression of C-C chemokine receptor 5 (CCR5) in the gut.
30429227	5	84	theme	B.	825:826	arg1	treatment					837:845	B. fragilis treatment	825:845	B. fragilis treatment	825:845	Further, we reveal a protective role of B. fragilis treatment against colon tumorigenesis using an azoxymethane (AOM)/DSS-induced model of colitis-associated colon cancer in mice and demonstrate that the decreased tumorigenesis by B. fragilis administration is accompanied by inhibited expression of C-C chemokine receptor 5 (CCR5) in the gut.
30429227	0	85	theme	Colitis-Associated	65:82	arg1	Cancer					95:100	Colitis-Associated Colorectal Cancer	65:100	Colitis-Associated Colorectal Cancer	65:100	The Protective Role of Bacteroides fragilis in a Murine Model of Colitis-Associated Colorectal Cancer.
30429227	5	86	theme	chemokine	1089:1097	arg1	receptor					1099:1106	C-C chemokine receptor 5	1085:1108	C-C chemokine receptor 5 (CCR5)	1085:1115	Further, we reveal a protective role of B. fragilis treatment against colon tumorigenesis using an azoxymethane (AOM)/DSS-induced model of colitis-associated colon cancer in mice and demonstrate that the decreased tumorigenesis by B. fragilis administration is accompanied by inhibited expression of C-C chemokine receptor 5 (CCR5) in the gut.
30429227	5	86	theme	chemokine	1089:1097	arg1	CCR5					1111:1114	CCR5	1111:1114	CCR5	1111:1114	Further, we reveal a protective role of B. fragilis treatment against colon tumorigenesis using an azoxymethane (AOM)/DSS-induced model of colitis-associated colon cancer in mice and demonstrate that the decreased tumorigenesis by B. fragilis administration is accompanied by inhibited expression of C-C chemokine receptor 5 (CCR5) in the gut.
30429227	1	87	theme	gut	150:152	arg1	inflammation					130:141	chronic inflammation	122:141	chronic inflammation of the gut	122:152	Many patients with chronic inflammation of the gut, such as that observed in inflammatory bowel disease (IBD), develop colorectal cancer (CRC).
30429227	0	88	theme	Cancer	95:100	arg1	Model					56:60	a Murine Model	47:60	a Murine Model of Colitis-Associated Colorectal Cancer	47:100	The Protective Role of Bacteroides fragilis in a Murine Model of Colitis-Associated Colorectal Cancer.
30429227	6	89	from	fragilis	1316:1323	arg1	production					1271:1280	the production	1267:1280	the production of polysaccharide A (PSA) from B. fragilis	1267:1323	We show direct evidence that the inhibition of tumor formation provided by B. fragilis in colitis-associated CRC animals was dependent on the production of polysaccharide A (PSA) from B. fragilis and that Toll-like receptor 2 (TLR2) signaling was responsible for the protective function of B. fragilisIMPORTANCE The incidence of colorectal cancer (CRC) is rapidly growing worldwide, and there is therefore a greater emphasis on studies of the treatment or prevention of CRC pathogenesis.
30429227	11	90	theme	inflammation-associated	2303:2325	arg1	development					2331:2341	inflammation-associated CRC development	2303:2341	inflammation-associated CRC development	2303:2341	Our results suggest that the commensal microorganism B. fragilis can be used to prevent inflammation-associated CRC development and may provide an effective therapeutic strategy for CRC.
30429227	5	91	theme	/DSS-induced	902:913	arg1	model					915:919	an azoxymethane (AOM)/DSS-induced model	881:919	an azoxymethane (AOM)/DSS-induced model of colitis-associated colon cancer in mice	881:962	Further, we reveal a protective role of B. fragilis treatment against colon tumorigenesis using an azoxymethane (AOM)/DSS-induced model of colitis-associated colon cancer in mice and demonstrate that the decreased tumorigenesis by B. fragilis administration is accompanied by inhibited expression of C-C chemokine receptor 5 (CCR5) in the gut.
30429227	10	92	theme	A	2167:2167	arg1	production					2169:2178	polysaccharide A production	2152:2178	polysaccharide A production	2152:2178	The molecular mechanism for protection against CRC provided by B. fragilis is dependent on polysaccharide A production and is mediated by TLR2 signaling.
30429227	9	93	from	expression	2005:2014	arg1	colon					2054:2058	the colon	2050:2058	the colon	2050:2058	The protective role against tumor formation provided by B. fragilis is associated with inhibition of expression of the chemokine receptor CCR5 in the colon.
30429227	5	94	theme	inhibited	1061:1069	arg1	expression					1071:1080	inhibited expression	1061:1080	inhibited expression of C-C chemokine receptor 5 (CCR5) in the gut	1061:1126	Further, we reveal a protective role of B. fragilis treatment against colon tumorigenesis using an azoxymethane (AOM)/DSS-induced model of colitis-associated colon cancer in mice and demonstrate that the decreased tumorigenesis by B. fragilis administration is accompanied by inhibited expression of C-C chemokine receptor 5 (CCR5) in the gut.
30429227	10	95	theme	TLR2	2199:2202	arg1	signaling					2204:2212	TLR2 signaling	2199:2212	TLR2 signaling	2199:2212	The molecular mechanism for protection against CRC provided by B. fragilis is dependent on polysaccharide A production and is mediated by TLR2 signaling.
30429227	2	96	theme	imbalanced	335:344	arg1	composition					346:356	an imbalanced composition	332:356	an imbalanced composition of intestinal microbiota	332:381	Recent studies have reported that the development of IBD and CRC partly results from an imbalanced composition of intestinal microbiota and that intestinal inflammation in these diseases can be modulated by the microbiota.
30429227	0	97	theme	Protective	4:13	arg1	Role					15:18	The Protective Role	0:18	The Protective Role of Bacteroides fragilis in a Murine Model of Colitis-Associated Colorectal Cancer	0:100	The Protective Role of Bacteroides fragilis in a Murine Model of Colitis-Associated Colorectal Cancer.
30429227	1	98	with	patients	108:115	arg1	inflammation					130:141	chronic inflammation	122:141	chronic inflammation of the gut	122:152	Many patients with chronic inflammation of the gut, such as that observed in inflammatory bowel disease (IBD), develop colorectal cancer (CRC).
30429227	4	99	theme	dextran	695:701	arg1	sodium					711:716	dextran sulfate sodium	695:716	dextran sulfate sodium (DSS) treatment	695:732	In this study, we found that gut inflammation caused by dextran sulfate sodium (DSS) treatment was inhibited by B. fragilis colonization in mice.
30429227	4	99	theme	dextran	695:701	arg1	DSS					719:721	DSS	719:721	DSS	719:721	In this study, we found that gut inflammation caused by dextran sulfate sodium (DSS) treatment was inhibited by B. fragilis colonization in mice.
30429227	11	100	theme	CRC	2327:2329	arg1	development					2331:2341	inflammation-associated CRC development	2303:2341	inflammation-associated CRC development	2303:2341	Our results suggest that the commensal microorganism B. fragilis can be used to prevent inflammation-associated CRC development and may provide an effective therapeutic strategy for CRC.
30429227	3	101	theme	disease	623:629	arg1	models					631:636	several animal disease models	608:636	several animal disease models	608:636	The human commensal Bacteroides fragilis is best exemplified playing a protective role against the development of experimental colitis in several animal disease models.
30429227	4	102	theme	sodium	711:716	arg1	treatment					724:732	dextran sulfate sodium (DSS) treatment	695:732	dextran sulfate sodium (DSS) treatment	695:732	In this study, we found that gut inflammation caused by dextran sulfate sodium (DSS) treatment was inhibited by B. fragilis colonization in mice.
30429227	2	103	theme	microbiota	372:381	arg1	composition					346:356	an imbalanced composition	332:356	an imbalanced composition of intestinal microbiota	332:381	Recent studies have reported that the development of IBD and CRC partly results from an imbalanced composition of intestinal microbiota and that intestinal inflammation in these diseases can be modulated by the microbiota.
30429227	0	104	theme	fragilis	35:42	arg1	Role					15:18	The Protective Role	0:18	The Protective Role of Bacteroides fragilis in a Murine Model of Colitis-Associated Colorectal Cancer	0:100	The Protective Role of Bacteroides fragilis in a Murine Model of Colitis-Associated Colorectal Cancer.
30429227	6	105	theme	protective	1396:1405	arg1	function					1407:1414	the protective function	1392:1414	the protective function of B. fragilisIMPORTANCE The incidence of colorectal cancer (CRC)	1392:1480	We show direct evidence that the inhibition of tumor formation provided by B. fragilis in colitis-associated CRC animals was dependent on the production of polysaccharide A (PSA) from B. fragilis and that Toll-like receptor 2 (TLR2) signaling was responsible for the protective function of B. fragilisIMPORTANCE The incidence of colorectal cancer (CRC) is rapidly growing worldwide, and there is therefore a greater emphasis on studies of the treatment or prevention of CRC pathogenesis.
30429227	8	106	dep	B.	1854:1855	arg1	fragilis					1857:1864	fragilis	1857:1864	fragilis	1857:1864	We demonstrate, using a mouse model of colitis-associated CRC, that human commensal B. fragilis protects against colon tumorigenesis.
30429227	3	107	theme	several	608:614	arg1	models					631:636	several animal disease models	608:636	several animal disease models	608:636	The human commensal Bacteroides fragilis is best exemplified playing a protective role against the development of experimental colitis in several animal disease models.
30429227	6	108	theme	Toll-like	1334:1342	arg1	receptor					1344:1351	Toll-like receptor 2	1334:1353	Toll-like receptor 2 (TLR2) signaling	1334:1370	We show direct evidence that the inhibition of tumor formation provided by B. fragilis in colitis-associated CRC animals was dependent on the production of polysaccharide A (PSA) from B. fragilis and that Toll-like receptor 2 (TLR2) signaling was responsible for the protective function of B. fragilisIMPORTANCE The incidence of colorectal cancer (CRC) is rapidly growing worldwide, and there is therefore a greater emphasis on studies of the treatment or prevention of CRC pathogenesis.
30429227	6	108	theme	Toll-like	1334:1342	arg1	TLR2					1356:1359	TLR2	1356:1359	TLR2	1356:1359	We show direct evidence that the inhibition of tumor formation provided by B. fragilis in colitis-associated CRC animals was dependent on the production of polysaccharide A (PSA) from B. fragilis and that Toll-like receptor 2 (TLR2) signaling was responsible for the protective function of B. fragilisIMPORTANCE The incidence of colorectal cancer (CRC) is rapidly growing worldwide, and there is therefore a greater emphasis on studies of the treatment or prevention of CRC pathogenesis.
30429227	9	109	from	inhibition	1991:2000	arg1	colon					2054:2058	the colon	2050:2058	the colon	2050:2058	The protective role against tumor formation provided by B. fragilis is associated with inhibition of expression of the chemokine receptor CCR5 in the colon.
30429227	9	110	theme	receptor	2033:2040	arg1	CCR5					2042:2045	the chemokine receptor CCR5	2019:2045	the chemokine receptor CCR5	2019:2045	The protective role against tumor formation provided by B. fragilis is associated with inhibition of expression of the chemokine receptor CCR5 in the colon.
30429227	6	111	theme	B.	1419:1420	arg1	incidence					1445:1453	B. fragilisIMPORTANCE The incidence	1419:1453	B. fragilisIMPORTANCE The incidence of colorectal cancer (CRC)	1419:1480	We show direct evidence that the inhibition of tumor formation provided by B. fragilis in colitis-associated CRC animals was dependent on the production of polysaccharide A (PSA) from B. fragilis and that Toll-like receptor 2 (TLR2) signaling was responsible for the protective function of B. fragilisIMPORTANCE The incidence of colorectal cancer (CRC) is rapidly growing worldwide, and there is therefore a greater emphasis on studies of the treatment or prevention of CRC pathogenesis.
30429227	5	112	theme	colon	943:947	arg1	cancer					949:954	colitis-associated colon cancer	924:954	colitis-associated colon cancer in mice	924:962	Further, we reveal a protective role of B. fragilis treatment against colon tumorigenesis using an azoxymethane (AOM)/DSS-induced model of colitis-associated colon cancer in mice and demonstrate that the decreased tumorigenesis by B. fragilis administration is accompanied by inhibited expression of C-C chemokine receptor 5 (CCR5) in the gut.
30429227	3	113	theme	commensal	480:488	arg1	fragilis					502:509	The human commensal Bacteroides fragilis	470:509	The human commensal Bacteroides fragilis	470:509	The human commensal Bacteroides fragilis is best exemplified playing a protective role against the development of experimental colitis in several animal disease models.
30429227	7	114	from	inflammation	1708:1719	arg1	tract					1763:1767	the gastrointestinal tract	1742:1767	the gastrointestinal tract	1742:1767	Recent studies suggested that consideration of the microbiota is unavoidable to understand inflammation and tumorigenesis in the gastrointestinal tract.
30429227	11	115	theme	B.	2268:2269	arg1	microorganism					2254:2266	the commensal microorganism	2240:2266	the commensal microorganism B. fragilis	2240:2278	Our results suggest that the commensal microorganism B. fragilis can be used to prevent inflammation-associated CRC development and may provide an effective therapeutic strategy for CRC.
30429227	4	116	theme	B.	751:752	arg1	colonization					763:774	B. fragilis colonization	751:774	B. fragilis colonization in mice	751:782	In this study, we found that gut inflammation caused by dextran sulfate sodium (DSS) treatment was inhibited by B. fragilis colonization in mice.
30429227	5	117	from	expression	1071:1080	arg1	gut					1124:1126	the gut	1120:1126	the gut	1120:1126	Further, we reveal a protective role of B. fragilis treatment against colon tumorigenesis using an azoxymethane (AOM)/DSS-induced model of colitis-associated colon cancer in mice and demonstrate that the decreased tumorigenesis by B. fragilis administration is accompanied by inhibited expression of C-C chemokine receptor 5 (CCR5) in the gut.
30838933	7	0	theme	physical	1205:1212	arg1	properties					1246:1255	good physical, chemical, and biocompatibility properties	1200:1255	good physical, chemical, and biocompatibility properties	1200:1255	Impact Statement In this study, we demonstrated that the silk fibroin-bacterial cellulose (SF-BC) membrane possessed good physical, chemical, and biocompatibility properties in vitro.
30838933	6	1	theme	uterus	956:961	arg1	arteriogenesis					922:935	arteriogenesis	922:935	arteriogenesis	922:935	The results showed that SF-BC loaded with rhSDF-1α significantly enhanced endometrial regeneration and arteriogenesis of the injured rat uterus, which led to improved pregnancy outcomes, thus indicating much promise for functional uterine repair and regeneration.
30838933	6	1	theme	uterus	956:961	arg1	regeneration					905:916	endometrial regeneration	893:916	endometrial regeneration	893:916	The results showed that SF-BC loaded with rhSDF-1α significantly enhanced endometrial regeneration and arteriogenesis of the injured rat uterus, which led to improved pregnancy outcomes, thus indicating much promise for functional uterine repair and regeneration.
30838933	9	2	theme	uterine	1648:1654	arg1	injury					1656:1661	uterine injury	1648:1661	uterine injury	1648:1661	The results thus suggest that SF-BC loaded with rhSDF-1α has good potential in future clinical applications for the repair of uterine injury.
30838933	1	3	theme	uterus	169:174	arg1	infection					130:138	Severe infection	123:138	Severe infection	123:138	Severe infection and mechanical injury of the uterus may lead to infertility and miscarriage.
30838933	1	3	theme	uterus	169:174	arg1	injury					155:160	mechanical injury	144:160	mechanical injury	144:160	Severe infection and mechanical injury of the uterus may lead to infertility and miscarriage.
30838933	6	4	theme	pregnancy	986:994	arg1	outcomes					996:1003	improved pregnancy outcomes	977:1003	improved pregnancy outcomes	977:1003	The results showed that SF-BC loaded with rhSDF-1α significantly enhanced endometrial regeneration and arteriogenesis of the injured rat uterus, which led to improved pregnancy outcomes, thus indicating much promise for functional uterine repair and regeneration.
30838933	5	5	theme	tissue	743:748	arg1	efficacy					763:770	The tissue regeneration efficacy	739:770	The tissue regeneration efficacy of the three groups	739:790	The tissue regeneration efficacy of the three groups was analyzed and compared.
30838933	6	6	theme	endometrial	893:903	arg1	regeneration					905:916	endometrial regeneration	893:916	endometrial regeneration	893:916	The results showed that SF-BC loaded with rhSDF-1α significantly enhanced endometrial regeneration and arteriogenesis of the injured rat uterus, which led to improved pregnancy outcomes, thus indicating much promise for functional uterine repair and regeneration.
30838933	9	7	theme	injury	1656:1661	arg1	repair					1638:1643	the repair	1634:1643	the repair of uterine injury	1634:1661	The results thus suggest that SF-BC loaded with rhSDF-1α has good potential in future clinical applications for the repair of uterine injury.
30838933	7	8	theme	biocompatibility	1229:1244	arg1	properties					1246:1255	good physical, chemical, and biocompatibility properties	1200:1255	good physical, chemical, and biocompatibility properties	1200:1255	Impact Statement In this study, we demonstrated that the silk fibroin-bacterial cellulose (SF-BC) membrane possessed good physical, chemical, and biocompatibility properties in vitro.
30838933	4	9	theme	injury	580:585	arg1	model					563:567	A rat model	557:567	A rat model of uterine injury	557:585	A rat model of uterine injury was utilized for this study, which was composed of three groups as follows: blank control, implantation with SF-BC only, or SF-BC loaded with rhSDF-1α.
30838933	8	10	theme	uterus	1514:1519	arg1	outcome					1491:1497	the pregnancy outcome	1477:1497	the pregnancy outcome of the damaged uterus	1477:1519	The in vivo study showed that the incorporation of recombinant human stromal cell-derived factor-1α (rhSDF-1α) within the SF-BC membrane promoted regeneration of full-thickness uterine injury and also improved the pregnancy outcome of the damaged uterus.
30838933	8	11	theme	stromal	1336:1342	arg1	rhSDF-1α					1368:1375	rhSDF-1α	1368:1375	rhSDF-1α	1368:1375	The in vivo study showed that the incorporation of recombinant human stromal cell-derived factor-1α (rhSDF-1α) within the SF-BC membrane promoted regeneration of full-thickness uterine injury and also improved the pregnancy outcome of the damaged uterus.
30838933	8	11	theme	stromal	1336:1342	arg1	factor-1α					1357:1365	recombinant human stromal cell-derived factor-1α	1318:1365	recombinant human stromal cell-derived factor-1α (rhSDF-1α)	1318:1376	The in vivo study showed that the incorporation of recombinant human stromal cell-derived factor-1α (rhSDF-1α) within the SF-BC membrane promoted regeneration of full-thickness uterine injury and also improved the pregnancy outcome of the damaged uterus.
30838933	2	12	theme	uterine	301:307	arg1	injury					309:314	uterine injury	301:314	uterine injury	301:314	Currently, there is a lack of effective treatment modality for functional repair of uterine injury.
30838933	0	13	theme	Uterus	108:113	arg1	Repair					115:120	the Uterus Repair	104:120	the Uterus Repair	104:120	Local Delivery of Silk-Cellulose Incorporated with Stromal Cell-Derived Factor-1α Functionally Improves the Uterus Repair.
30838933	4	14	with	implantation	678:689	arg1	SF-BC					696:700	SF-BC	696:700	SF-BC only	696:705	A rat model of uterine injury was utilized for this study, which was composed of three groups as follows: blank control, implantation with SF-BC only, or SF-BC loaded with rhSDF-1α.
30838933	8	15	theme	human	1330:1334	arg1	rhSDF-1α					1368:1375	rhSDF-1α	1368:1375	rhSDF-1α	1368:1375	The in vivo study showed that the incorporation of recombinant human stromal cell-derived factor-1α (rhSDF-1α) within the SF-BC membrane promoted regeneration of full-thickness uterine injury and also improved the pregnancy outcome of the damaged uterus.
30838933	8	15	theme	human	1330:1334	arg1	factor-1α					1357:1365	recombinant human stromal cell-derived factor-1α	1318:1365	recombinant human stromal cell-derived factor-1α (rhSDF-1α)	1318:1376	The in vivo study showed that the incorporation of recombinant human stromal cell-derived factor-1α (rhSDF-1α) within the SF-BC membrane promoted regeneration of full-thickness uterine injury and also improved the pregnancy outcome of the damaged uterus.
30838933	3	16	theme	cellulose	521:529	arg1	carrier					548:554	a silk fibroin-bacterial cellulose (SF-BC) membrane carrier	496:554	a silk fibroin-bacterial cellulose (SF-BC) membrane carrier	496:554	To address this clinical challenge, this study aimed to develop a chemotactic composite scaffold by incorporating recombinant human stromal cell-derived factor-1α (rhSDF-1α) into a silk fibroin-bacterial cellulose (SF-BC) membrane carrier.
30838933	9	17	contain	has	1579:1581	arg2	potential					1588:1596	good potential	1583:1596	good potential	1583:1596	The results thus suggest that SF-BC loaded with rhSDF-1α has good potential in future clinical applications for the repair of uterine injury.
30838933	9	17	contain	has	1579:1581	arg1	SF-BC					1552:1556	SF-BC	1552:1556	SF-BC loaded with rhSDF-1α	1552:1577	The results thus suggest that SF-BC loaded with rhSDF-1α has good potential in future clinical applications for the repair of uterine injury.
30838933	2	18	theme	functional	280:289	arg1	repair					291:296	functional repair	280:296	functional repair of uterine injury	280:314	Currently, there is a lack of effective treatment modality for functional repair of uterine injury.
30838933	3	19	theme	stromal	449:455	arg1	rhSDF-1α					481:488	rhSDF-1α	481:488	rhSDF-1α	481:488	To address this clinical challenge, this study aimed to develop a chemotactic composite scaffold by incorporating recombinant human stromal cell-derived factor-1α (rhSDF-1α) into a silk fibroin-bacterial cellulose (SF-BC) membrane carrier.
30838933	3	19	theme	stromal	449:455	arg1	factor-1α					470:478	recombinant human stromal cell-derived factor-1α	431:478	recombinant human stromal cell-derived factor-1α (rhSDF-1α)	431:489	To address this clinical challenge, this study aimed to develop a chemotactic composite scaffold by incorporating recombinant human stromal cell-derived factor-1α (rhSDF-1α) into a silk fibroin-bacterial cellulose (SF-BC) membrane carrier.
30838933	4	20	with	control	669:675	arg1	SF-BC					696:700	SF-BC	696:700	SF-BC only	696:705	A rat model of uterine injury was utilized for this study, which was composed of three groups as follows: blank control, implantation with SF-BC only, or SF-BC loaded with rhSDF-1α.
30838933	0	21	theme	Local	0:4	arg1	Delivery					6:13	Local Delivery	0:13	Local Delivery of Silk-Cellulose Incorporated with Stromal Cell-Derived Factor-1α	0:80	Local Delivery of Silk-Cellulose Incorporated with Stromal Cell-Derived Factor-1α Functionally Improves the Uterus Repair.
30838933	3	22	theme	cell-derived	457:468	arg1	rhSDF-1α					481:488	rhSDF-1α	481:488	rhSDF-1α	481:488	To address this clinical challenge, this study aimed to develop a chemotactic composite scaffold by incorporating recombinant human stromal cell-derived factor-1α (rhSDF-1α) into a silk fibroin-bacterial cellulose (SF-BC) membrane carrier.
30838933	3	22	theme	cell-derived	457:468	arg1	factor-1α					470:478	recombinant human stromal cell-derived factor-1α	431:478	recombinant human stromal cell-derived factor-1α (rhSDF-1α)	431:489	To address this clinical challenge, this study aimed to develop a chemotactic composite scaffold by incorporating recombinant human stromal cell-derived factor-1α (rhSDF-1α) into a silk fibroin-bacterial cellulose (SF-BC) membrane carrier.
30838933	7	23	theme	chemical	1215:1222	arg1	properties					1246:1255	good physical, chemical, and biocompatibility properties	1200:1255	good physical, chemical, and biocompatibility properties	1200:1255	Impact Statement In this study, we demonstrated that the silk fibroin-bacterial cellulose (SF-BC) membrane possessed good physical, chemical, and biocompatibility properties in vitro.
30838933	3	24	theme	recombinant	431:441	arg1	rhSDF-1α					481:488	rhSDF-1α	481:488	rhSDF-1α	481:488	To address this clinical challenge, this study aimed to develop a chemotactic composite scaffold by incorporating recombinant human stromal cell-derived factor-1α (rhSDF-1α) into a silk fibroin-bacterial cellulose (SF-BC) membrane carrier.
30838933	3	24	theme	recombinant	431:441	arg1	factor-1α					470:478	recombinant human stromal cell-derived factor-1α	431:478	recombinant human stromal cell-derived factor-1α (rhSDF-1α)	431:489	To address this clinical challenge, this study aimed to develop a chemotactic composite scaffold by incorporating recombinant human stromal cell-derived factor-1α (rhSDF-1α) into a silk fibroin-bacterial cellulose (SF-BC) membrane carrier.
30838933	3	25	theme	chemotactic	383:393	arg1	scaffold					405:412	a chemotactic composite scaffold	381:412	a chemotactic composite scaffold	381:412	To address this clinical challenge, this study aimed to develop a chemotactic composite scaffold by incorporating recombinant human stromal cell-derived factor-1α (rhSDF-1α) into a silk fibroin-bacterial cellulose (SF-BC) membrane carrier.
30838933	3	26	link	cell-derived	457:468	arg1	rhSDF-1α					481:488	rhSDF-1α	481:488	rhSDF-1α	481:488	To address this clinical challenge, this study aimed to develop a chemotactic composite scaffold by incorporating recombinant human stromal cell-derived factor-1α (rhSDF-1α) into a silk fibroin-bacterial cellulose (SF-BC) membrane carrier.
30838933	3	26	link	cell-derived	457:468	arg1	factor-1α					470:478	recombinant human stromal cell-derived factor-1α	431:478	recombinant human stromal cell-derived factor-1α (rhSDF-1α)	431:489	To address this clinical challenge, this study aimed to develop a chemotactic composite scaffold by incorporating recombinant human stromal cell-derived factor-1α (rhSDF-1α) into a silk fibroin-bacterial cellulose (SF-BC) membrane carrier.
30838933	5	27	theme	groups	785:790	arg1	efficacy					763:770	The tissue regeneration efficacy	739:770	The tissue regeneration efficacy of the three groups	739:790	The tissue regeneration efficacy of the three groups was analyzed and compared.
30838933	8	28	theme	factor-1α	1357:1365	arg1	incorporation					1301:1313	the incorporation	1297:1313	the incorporation of recombinant human stromal cell-derived factor-1α (rhSDF-1α) within the SF-BC membrane	1297:1402	The in vivo study showed that the incorporation of recombinant human stromal cell-derived factor-1α (rhSDF-1α) within the SF-BC membrane promoted regeneration of full-thickness uterine injury and also improved the pregnancy outcome of the damaged uterus.
30838933	3	29	theme	human	443:447	arg1	rhSDF-1α					481:488	rhSDF-1α	481:488	rhSDF-1α	481:488	To address this clinical challenge, this study aimed to develop a chemotactic composite scaffold by incorporating recombinant human stromal cell-derived factor-1α (rhSDF-1α) into a silk fibroin-bacterial cellulose (SF-BC) membrane carrier.
30838933	3	29	theme	human	443:447	arg1	factor-1α					470:478	recombinant human stromal cell-derived factor-1α	431:478	recombinant human stromal cell-derived factor-1α (rhSDF-1α)	431:489	To address this clinical challenge, this study aimed to develop a chemotactic composite scaffold by incorporating recombinant human stromal cell-derived factor-1α (rhSDF-1α) into a silk fibroin-bacterial cellulose (SF-BC) membrane carrier.
30838933	0	30	theme	Silk-Cellulose	18:31	arg1	Delivery					6:13	Local Delivery	0:13	Local Delivery of Silk-Cellulose Incorporated with Stromal Cell-Derived Factor-1α	0:80	Local Delivery of Silk-Cellulose Incorporated with Stromal Cell-Derived Factor-1α Functionally Improves the Uterus Repair.
30838933	4	31	theme	rat	559:561	arg1	model					563:567	A rat model	557:567	A rat model of uterine injury	557:585	A rat model of uterine injury was utilized for this study, which was composed of three groups as follows: blank control, implantation with SF-BC only, or SF-BC loaded with rhSDF-1α.
30838933	2	32	theme	injury	309:314	arg1	repair					291:296	functional repair	280:296	functional repair of uterine injury	280:314	Currently, there is a lack of effective treatment modality for functional repair of uterine injury.
30838933	6	33	theme	functional	1039:1048	arg1	repair					1058:1063	functional uterine repair	1039:1063	functional uterine repair	1039:1063	The results showed that SF-BC loaded with rhSDF-1α significantly enhanced endometrial regeneration and arteriogenesis of the injured rat uterus, which led to improved pregnancy outcomes, thus indicating much promise for functional uterine repair and regeneration.
30838933	7	34	theme	cellulose	1163:1171	arg1	membrane					1181:1188	the silk fibroin-bacterial cellulose (SF-BC) membrane	1136:1188	the silk fibroin-bacterial cellulose (SF-BC) membrane	1136:1188	Impact Statement In this study, we demonstrated that the silk fibroin-bacterial cellulose (SF-BC) membrane possessed good physical, chemical, and biocompatibility properties in vitro.
30838933	4	35	theme	uterine	572:578	arg1	injury					580:585	uterine injury	572:585	uterine injury	572:585	A rat model of uterine injury was utilized for this study, which was composed of three groups as follows: blank control, implantation with SF-BC only, or SF-BC loaded with rhSDF-1α.
30838933	3	36	theme	membrane	539:546	arg1	carrier					548:554	a silk fibroin-bacterial cellulose (SF-BC) membrane carrier	496:554	a silk fibroin-bacterial cellulose (SF-BC) membrane carrier	496:554	To address this clinical challenge, this study aimed to develop a chemotactic composite scaffold by incorporating recombinant human stromal cell-derived factor-1α (rhSDF-1α) into a silk fibroin-bacterial cellulose (SF-BC) membrane carrier.
30838933	1	37	theme	Severe	123:128	arg1	infection					130:138	Severe infection	123:138	Severe infection	123:138	Severe infection and mechanical injury of the uterus may lead to infertility and miscarriage.
30838933	8	38	theme	uterine	1444:1450	arg1	injury					1452:1457	full-thickness uterine injury	1429:1457	full-thickness uterine injury	1429:1457	The in vivo study showed that the incorporation of recombinant human stromal cell-derived factor-1α (rhSDF-1α) within the SF-BC membrane promoted regeneration of full-thickness uterine injury and also improved the pregnancy outcome of the damaged uterus.
30838933	5	39	theme	regeneration	750:761	arg1	efficacy					763:770	The tissue regeneration efficacy	739:770	The tissue regeneration efficacy of the three groups	739:790	The tissue regeneration efficacy of the three groups was analyzed and compared.
30838933	6	40	theme	rat	952:954	arg1	uterus					956:961	the injured rat uterus	940:961	the injured rat uterus	940:961	The results showed that SF-BC loaded with rhSDF-1α significantly enhanced endometrial regeneration and arteriogenesis of the injured rat uterus, which led to improved pregnancy outcomes, thus indicating much promise for functional uterine repair and regeneration.
30838933	7	41	theme	SF-BC	1174:1178	arg1	membrane					1181:1188	the silk fibroin-bacterial cellulose (SF-BC) membrane	1136:1188	the silk fibroin-bacterial cellulose (SF-BC) membrane	1136:1188	Impact Statement In this study, we demonstrated that the silk fibroin-bacterial cellulose (SF-BC) membrane possessed good physical, chemical, and biocompatibility properties in vitro.
30838933	8	42	theme	SF-BC	1389:1393	arg1	membrane					1395:1402	the SF-BC membrane	1385:1402	the SF-BC membrane	1385:1402	The in vivo study showed that the incorporation of recombinant human stromal cell-derived factor-1α (rhSDF-1α) within the SF-BC membrane promoted regeneration of full-thickness uterine injury and also improved the pregnancy outcome of the damaged uterus.
30838933	6	43	theme	uterine	1050:1056	arg1	repair					1058:1063	functional uterine repair	1039:1063	functional uterine repair	1039:1063	The results showed that SF-BC loaded with rhSDF-1α significantly enhanced endometrial regeneration and arteriogenesis of the injured rat uterus, which led to improved pregnancy outcomes, thus indicating much promise for functional uterine repair and regeneration.
30838933	8	44	theme	in	1271:1272	arg1	study					1279:1283	The in vivo study	1267:1283	The in vivo study	1267:1283	The in vivo study showed that the incorporation of recombinant human stromal cell-derived factor-1α (rhSDF-1α) within the SF-BC membrane promoted regeneration of full-thickness uterine injury and also improved the pregnancy outcome of the damaged uterus.
30838933	7	45	theme	silk	1140:1143	arg1	membrane					1181:1188	the silk fibroin-bacterial cellulose (SF-BC) membrane	1136:1188	the silk fibroin-bacterial cellulose (SF-BC) membrane	1136:1188	Impact Statement In this study, we demonstrated that the silk fibroin-bacterial cellulose (SF-BC) membrane possessed good physical, chemical, and biocompatibility properties in vitro.
30838933	6	46	theme	injured	944:950	arg1	uterus					956:961	the injured rat uterus	940:961	the injured rat uterus	940:961	The results showed that SF-BC loaded with rhSDF-1α significantly enhanced endometrial regeneration and arteriogenesis of the injured rat uterus, which led to improved pregnancy outcomes, thus indicating much promise for functional uterine repair and regeneration.
30838933	6	47	theme	improved	977:984	arg1	outcomes					996:1003	improved pregnancy outcomes	977:1003	improved pregnancy outcomes	977:1003	The results showed that SF-BC loaded with rhSDF-1α significantly enhanced endometrial regeneration and arteriogenesis of the injured rat uterus, which led to improved pregnancy outcomes, thus indicating much promise for functional uterine repair and regeneration.
30838933	7	48	theme	fibroin-bacterial	1145:1161	arg1	membrane					1181:1188	the silk fibroin-bacterial cellulose (SF-BC) membrane	1136:1188	the silk fibroin-bacterial cellulose (SF-BC) membrane	1136:1188	Impact Statement In this study, we demonstrated that the silk fibroin-bacterial cellulose (SF-BC) membrane possessed good physical, chemical, and biocompatibility properties in vitro.
30838933	6	49	theme	much	1022:1025	arg1	promise					1027:1033	much promise	1022:1033	much promise for functional uterine repair and regeneration	1022:1080	The results showed that SF-BC loaded with rhSDF-1α significantly enhanced endometrial regeneration and arteriogenesis of the injured rat uterus, which led to improved pregnancy outcomes, thus indicating much promise for functional uterine repair and regeneration.
30838933	0	50	theme	Cell-Derived	59:70	arg1	Factor-1α					72:80	Stromal Cell-Derived Factor-1α	51:80	Stromal Cell-Derived Factor-1α	51:80	Local Delivery of Silk-Cellulose Incorporated with Stromal Cell-Derived Factor-1α Functionally Improves the Uterus Repair.
30838933	4	51	used	utilized	591:598	arg2	model					563:567	A rat model	557:567	A rat model of uterine injury	557:585	A rat model of uterine injury was utilized for this study, which was composed of three groups as follows: blank control, implantation with SF-BC only, or SF-BC loaded with rhSDF-1α.
30838933	8	52	link	cell-derived	1344:1355	arg1	rhSDF-1α					1368:1375	rhSDF-1α	1368:1375	rhSDF-1α	1368:1375	The in vivo study showed that the incorporation of recombinant human stromal cell-derived factor-1α (rhSDF-1α) within the SF-BC membrane promoted regeneration of full-thickness uterine injury and also improved the pregnancy outcome of the damaged uterus.
30838933	8	52	link	cell-derived	1344:1355	arg1	factor-1α					1357:1365	recombinant human stromal cell-derived factor-1α	1318:1365	recombinant human stromal cell-derived factor-1α (rhSDF-1α)	1318:1376	The in vivo study showed that the incorporation of recombinant human stromal cell-derived factor-1α (rhSDF-1α) within the SF-BC membrane promoted regeneration of full-thickness uterine injury and also improved the pregnancy outcome of the damaged uterus.
30838933	9	53	theme	clinical	1608:1615	arg1	applications					1617:1628	future clinical applications	1601:1628	future clinical applications for the repair of uterine injury	1601:1661	The results thus suggest that SF-BC loaded with rhSDF-1α has good potential in future clinical applications for the repair of uterine injury.
30838933	3	54	theme	silk	498:501	arg1	carrier					548:554	a silk fibroin-bacterial cellulose (SF-BC) membrane carrier	496:554	a silk fibroin-bacterial cellulose (SF-BC) membrane carrier	496:554	To address this clinical challenge, this study aimed to develop a chemotactic composite scaffold by incorporating recombinant human stromal cell-derived factor-1α (rhSDF-1α) into a silk fibroin-bacterial cellulose (SF-BC) membrane carrier.
30838933	0	55	theme	Stromal	51:57	arg1	Factor-1α					72:80	Stromal Cell-Derived Factor-1α	51:80	Stromal Cell-Derived Factor-1α	51:80	Local Delivery of Silk-Cellulose Incorporated with Stromal Cell-Derived Factor-1α Functionally Improves the Uterus Repair.
30838933	4	56	with	SF-BC	711:715	arg1	SF-BC					696:700	SF-BC	696:700	SF-BC only	696:705	A rat model of uterine injury was utilized for this study, which was composed of three groups as follows: blank control, implantation with SF-BC only, or SF-BC loaded with rhSDF-1α.
30838933	8	57	theme	pregnancy	1481:1489	arg1	outcome					1491:1497	the pregnancy outcome	1477:1497	the pregnancy outcome of the damaged uterus	1477:1519	The in vivo study showed that the incorporation of recombinant human stromal cell-derived factor-1α (rhSDF-1α) within the SF-BC membrane promoted regeneration of full-thickness uterine injury and also improved the pregnancy outcome of the damaged uterus.
30838933	3	58	theme	fibroin-bacterial	503:519	arg1	carrier					548:554	a silk fibroin-bacterial cellulose (SF-BC) membrane carrier	496:554	a silk fibroin-bacterial cellulose (SF-BC) membrane carrier	496:554	To address this clinical challenge, this study aimed to develop a chemotactic composite scaffold by incorporating recombinant human stromal cell-derived factor-1α (rhSDF-1α) into a silk fibroin-bacterial cellulose (SF-BC) membrane carrier.
30838933	8	59	theme	injury	1452:1457	arg1	regeneration					1413:1424	regeneration	1413:1424	regeneration of full-thickness uterine injury	1413:1457	The in vivo study showed that the incorporation of recombinant human stromal cell-derived factor-1α (rhSDF-1α) within the SF-BC membrane promoted regeneration of full-thickness uterine injury and also improved the pregnancy outcome of the damaged uterus.
30838933	8	60	theme	full-thickness	1429:1442	arg1	injury					1452:1457	full-thickness uterine injury	1429:1457	full-thickness uterine injury	1429:1457	The in vivo study showed that the incorporation of recombinant human stromal cell-derived factor-1α (rhSDF-1α) within the SF-BC membrane promoted regeneration of full-thickness uterine injury and also improved the pregnancy outcome of the damaged uterus.
30838933	2	61	theme	modality	267:274	arg1	lack					239:242	a lack	237:242	a lack of effective treatment modality for functional repair of uterine injury	237:314	Currently, there is a lack of effective treatment modality for functional repair of uterine injury.
30838933	7	62	contain	possessed	1190:1198	arg1	membrane					1181:1188	the silk fibroin-bacterial cellulose (SF-BC) membrane	1136:1188	the silk fibroin-bacterial cellulose (SF-BC) membrane	1136:1188	Impact Statement In this study, we demonstrated that the silk fibroin-bacterial cellulose (SF-BC) membrane possessed good physical, chemical, and biocompatibility properties in vitro.
30838933	7	62	contain	possessed	1190:1198	arg2	properties					1246:1255	good physical, chemical, and biocompatibility properties	1200:1255	good physical, chemical, and biocompatibility properties	1200:1255	Impact Statement In this study, we demonstrated that the silk fibroin-bacterial cellulose (SF-BC) membrane possessed good physical, chemical, and biocompatibility properties in vitro.
30838933	1	63	theme	mechanical	144:153	arg1	injury					155:160	mechanical injury	144:160	mechanical injury	144:160	Severe infection and mechanical injury of the uterus may lead to infertility and miscarriage.
30838933	9	64	theme	good	1583:1586	arg1	potential					1588:1596	good potential	1583:1596	good potential	1583:1596	The results thus suggest that SF-BC loaded with rhSDF-1α has good potential in future clinical applications for the repair of uterine injury.
30838933	8	65	theme	cell-derived	1344:1355	arg1	rhSDF-1α					1368:1375	rhSDF-1α	1368:1375	rhSDF-1α	1368:1375	The in vivo study showed that the incorporation of recombinant human stromal cell-derived factor-1α (rhSDF-1α) within the SF-BC membrane promoted regeneration of full-thickness uterine injury and also improved the pregnancy outcome of the damaged uterus.
30838933	8	65	theme	cell-derived	1344:1355	arg1	factor-1α					1357:1365	recombinant human stromal cell-derived factor-1α	1318:1365	recombinant human stromal cell-derived factor-1α (rhSDF-1α)	1318:1376	The in vivo study showed that the incorporation of recombinant human stromal cell-derived factor-1α (rhSDF-1α) within the SF-BC membrane promoted regeneration of full-thickness uterine injury and also improved the pregnancy outcome of the damaged uterus.
30838933	2	66	theme	treatment	257:265	arg1	modality					267:274	effective treatment modality	247:274	effective treatment modality for functional repair of uterine injury	247:314	Currently, there is a lack of effective treatment modality for functional repair of uterine injury.
30838933	8	67	dep	in	1271:1272	arg1	vivo					1274:1277	vivo	1274:1277	vivo	1274:1277	The in vivo study showed that the incorporation of recombinant human stromal cell-derived factor-1α (rhSDF-1α) within the SF-BC membrane promoted regeneration of full-thickness uterine injury and also improved the pregnancy outcome of the damaged uterus.
30838933	8	68	theme	recombinant	1318:1328	arg1	rhSDF-1α					1368:1375	rhSDF-1α	1368:1375	rhSDF-1α	1368:1375	The in vivo study showed that the incorporation of recombinant human stromal cell-derived factor-1α (rhSDF-1α) within the SF-BC membrane promoted regeneration of full-thickness uterine injury and also improved the pregnancy outcome of the damaged uterus.
30838933	8	68	theme	recombinant	1318:1328	arg1	factor-1α					1357:1365	recombinant human stromal cell-derived factor-1α	1318:1365	recombinant human stromal cell-derived factor-1α (rhSDF-1α)	1318:1376	The in vivo study showed that the incorporation of recombinant human stromal cell-derived factor-1α (rhSDF-1α) within the SF-BC membrane promoted regeneration of full-thickness uterine injury and also improved the pregnancy outcome of the damaged uterus.
30838933	7	69	theme	Impact	1083:1088	arg1	Statement					1090:1098	Impact Statement	1083:1098	Impact Statement	1083:1098	Impact Statement In this study, we demonstrated that the silk fibroin-bacterial cellulose (SF-BC) membrane possessed good physical, chemical, and biocompatibility properties in vitro.
30838933	3	70	theme	clinical	333:340	arg1	challenge					342:350	this clinical challenge	328:350	this clinical challenge	328:350	To address this clinical challenge, this study aimed to develop a chemotactic composite scaffold by incorporating recombinant human stromal cell-derived factor-1α (rhSDF-1α) into a silk fibroin-bacterial cellulose (SF-BC) membrane carrier.
30838933	2	71	theme	effective	247:255	arg1	modality					267:274	effective treatment modality	247:274	effective treatment modality for functional repair of uterine injury	247:314	Currently, there is a lack of effective treatment modality for functional repair of uterine injury.
30838933	3	72	theme	SF-BC	532:536	arg1	carrier					548:554	a silk fibroin-bacterial cellulose (SF-BC) membrane carrier	496:554	a silk fibroin-bacterial cellulose (SF-BC) membrane carrier	496:554	To address this clinical challenge, this study aimed to develop a chemotactic composite scaffold by incorporating recombinant human stromal cell-derived factor-1α (rhSDF-1α) into a silk fibroin-bacterial cellulose (SF-BC) membrane carrier.
30838933	9	73	theme	future	1601:1606	arg1	applications					1617:1628	future clinical applications	1601:1628	future clinical applications for the repair of uterine injury	1601:1661	The results thus suggest that SF-BC loaded with rhSDF-1α has good potential in future clinical applications for the repair of uterine injury.
30838933	3	74	theme	composite	395:403	arg1	scaffold					405:412	a chemotactic composite scaffold	381:412	a chemotactic composite scaffold	381:412	To address this clinical challenge, this study aimed to develop a chemotactic composite scaffold by incorporating recombinant human stromal cell-derived factor-1α (rhSDF-1α) into a silk fibroin-bacterial cellulose (SF-BC) membrane carrier.
30838933	8	75	theme	damaged	1506:1512	arg1	uterus					1514:1519	the damaged uterus	1502:1519	the damaged uterus	1502:1519	The in vivo study showed that the incorporation of recombinant human stromal cell-derived factor-1α (rhSDF-1α) within the SF-BC membrane promoted regeneration of full-thickness uterine injury and also improved the pregnancy outcome of the damaged uterus.
30838933	4	76	dep	groups	644:649	arg1	implantation					678:689	implantation	678:689	implantation with SF-BC only	678:705	A rat model of uterine injury was utilized for this study, which was composed of three groups as follows: blank control, implantation with SF-BC only, or SF-BC loaded with rhSDF-1α.
30838933	4	76	dep	groups	644:649	arg1	control					669:675	blank control	663:675	blank control	663:675	A rat model of uterine injury was utilized for this study, which was composed of three groups as follows: blank control, implantation with SF-BC only, or SF-BC loaded with rhSDF-1α.
30838933	4	76	dep	groups	644:649	arg1	groups					644:649	three groups	638:649	three groups as follows: blank control, implantation with SF-BC only, or SF-BC loaded with rhSDF-1α	638:736	A rat model of uterine injury was utilized for this study, which was composed of three groups as follows: blank control, implantation with SF-BC only, or SF-BC loaded with rhSDF-1α.
30838933	4	76	dep	groups	644:649	arg1	SF-BC					711:715	SF-BC	711:715	SF-BC loaded with rhSDF-1α	711:736	A rat model of uterine injury was utilized for this study, which was composed of three groups as follows: blank control, implantation with SF-BC only, or SF-BC loaded with rhSDF-1α.
30838933	4	77	theme	blank	663:667	arg1	control					669:675	blank control	663:675	blank control	663:675	A rat model of uterine injury was utilized for this study, which was composed of three groups as follows: blank control, implantation with SF-BC only, or SF-BC loaded with rhSDF-1α.
30838933	4	77	theme	blank	663:667	arg1	groups					644:649	three groups	638:649	three groups as follows: blank control, implantation with SF-BC only, or SF-BC loaded with rhSDF-1α	638:736	A rat model of uterine injury was utilized for this study, which was composed of three groups as follows: blank control, implantation with SF-BC only, or SF-BC loaded with rhSDF-1α.
30838933	7	78	theme	good	1200:1203	arg1	properties					1246:1255	good physical, chemical, and biocompatibility properties	1200:1255	good physical, chemical, and biocompatibility properties	1200:1255	Impact Statement In this study, we demonstrated that the silk fibroin-bacterial cellulose (SF-BC) membrane possessed good physical, chemical, and biocompatibility properties in vitro.
29764229	3	0	theme	MPH	695:697	arg1	powder					710:715	MPH hemostatic powder	695:715	MPH hemostatic powder	695:715	OBJECTIVE The aim of our study is to compare the rate of complications in patients who underwent cardiac implantable electronic device (CIED) implantation utilizing MPH hemostatic powder to the rate of complications in those patients who underwent standard procedure protocol without MPH hemostatic powder.
29764229	3	1	from	patients	636:643	arg1	rate					605:608	the rate	601:608	the rate of complications in those patients who underwent standard procedure protocol without MPH hemostatic powder	601:715	OBJECTIVE The aim of our study is to compare the rate of complications in patients who underwent cardiac implantable electronic device (CIED) implantation utilizing MPH hemostatic powder to the rate of complications in those patients who underwent standard procedure protocol without MPH hemostatic powder.
29764229	2	2	theme	wide	290:293	arg1	tools					306:310	tools	306:310	tools for management of intraoperative bleeding	306:352	To date, there is a wide variety of tools for management of intraoperative bleeding but it still remains unclear what methods are preferred.
29764229	2	2	theme	wide	290:293	arg1	variety					295:301	a wide variety	288:301	a wide variety of tools for management of intraoperative bleeding	288:352	To date, there is a wide variety of tools for management of intraoperative bleeding but it still remains unclear what methods are preferred.
29764229	3	3	theme	device	539:544	arg1	implantation					553:564	cardiac implantable electronic device (CIED) implantation	508:564	cardiac implantable electronic device (CIED) implantation utilizing MPH hemostatic powder to the rate of complications in those patients who underwent standard procedure protocol without MPH hemostatic powder	508:715	OBJECTIVE The aim of our study is to compare the rate of complications in patients who underwent cardiac implantable electronic device (CIED) implantation utilizing MPH hemostatic powder to the rate of complications in those patients who underwent standard procedure protocol without MPH hemostatic powder.
29764229	4	4	used	used	818:821	arg2	METHODS					718:724	METHODS	718:724	METHODS In our study, a new plant-derived microporous polysaccharide hemostatic powder (Arista)	718:812	METHODS In our study, a new plant-derived microporous polysaccharide hemostatic powder (Arista) was used.
29764229	1	5	theme	potential	185:193	arg1	complications					195:207	potential complications	185:207	potential complications after electrophysiology (EP) device implantation procedures	185:267	BACKGROUND Surgical site bleeding and infection are potential complications after electrophysiology (EP) device implantation procedures.
29764229	1	5	theme	potential	185:193	arg1	bleeding					158:165	bleeding	158:165	bleeding	158:165	BACKGROUND Surgical site bleeding and infection are potential complications after electrophysiology (EP) device implantation procedures.
29764229	1	5	theme	potential	185:193	arg1	infection					171:179	infection	171:179	infection	171:179	BACKGROUND Surgical site bleeding and infection are potential complications after electrophysiology (EP) device implantation procedures.
29764229	3	6	theme	MPH	576:578	arg1	powder					591:596	MPH hemostatic powder	576:596	MPH hemostatic powder	576:596	OBJECTIVE The aim of our study is to compare the rate of complications in patients who underwent cardiac implantable electronic device (CIED) implantation utilizing MPH hemostatic powder to the rate of complications in those patients who underwent standard procedure protocol without MPH hemostatic powder.
29764229	11	7	theme	rates	1962:1966	arg1	reduction					1890:1898	reduction	1890:1898	reduction of the infections rates and device implantation site hematoma rates	1890:1966	CONCLUSION Using MPH hemostatic powder for post-procedural hemostasis was shown to result in a significant reduction in the rate of overall post-procedural complications (a composite endpoint of hematoma and infections), and a trend in reduction of the infections rates and device implantation site hematoma rates.
29764229	0	8	theme	infection	122:130	arg1	rates					93:97	postoperative rates	79:97	postoperative rates of pocket hematoma and infection	79:130	Use of MPH hemostatic powder for electrophysiology device implantation reduces postoperative rates of pocket hematoma and infection.
29764229	5	9	theme	MPH	1053:1055	arg1	powder					1068:1073	no MPH hemostatic powder	1050:1073	no MPH hemostatic powder	1050:1073	A total of 283 consecutive patients were retrospectively studied to assess the rate of complications in patients who underwent CIED implantation with MPH hemostatic powder (n = 77, MPH hemostatic powder) and without (n = 206, no MPH hemostatic powder).
29764229	5	9	theme	MPH	1053:1055	arg1	n = 206					1041:1047	n = 206	1041:1047	n = 206	1041:1047	A total of 283 consecutive patients were retrospectively studied to assess the rate of complications in patients who underwent CIED implantation with MPH hemostatic powder (n = 77, MPH hemostatic powder) and without (n = 206, no MPH hemostatic powder).
29764229	8	10	from	group	1358:1362	arg1	%					1371:1371	0.4%	1368:1371	0.4%	1368:1371	The rate of device implantation site MPH hematoma in the MPH hemostatic powder group was 0.4%, versus 0.9% in the other group.
29764229	8	10	from	group	1358:1362	arg1	rate					1283:1286	The rate	1279:1286	The rate of device implantation site MPH hematoma in the MPH hemostatic powder group	1279:1362	The rate of device implantation site MPH hematoma in the MPH hemostatic powder group was 0.4%, versus 0.9% in the other group.
29764229	11	11	theme	site	1948:1951	arg1	rates					1962:1966	device implantation site hematoma rates	1928:1966	device implantation site hematoma rates	1928:1966	CONCLUSION Using MPH hemostatic powder for post-procedural hemostasis was shown to result in a significant reduction in the rate of overall post-procedural complications (a composite endpoint of hematoma and infections), and a trend in reduction of the infections rates and device implantation site hematoma rates.
29764229	11	12	theme	overall	1786:1792	arg1	complications					1810:1822	overall post-procedural complications	1786:1822	overall post-procedural complications (a composite endpoint of hematoma and infections)	1786:1872	CONCLUSION Using MPH hemostatic powder for post-procedural hemostasis was shown to result in a significant reduction in the rate of overall post-procedural complications (a composite endpoint of hematoma and infections), and a trend in reduction of the infections rates and device implantation site hematoma rates.
29764229	7	13	theme	powder	1142:1147	arg1	group					1149:1153	The MPH hemostatic powder group	1123:1153	The MPH hemostatic powder group of patients	1123:1165	RESULTS The MPH hemostatic powder group of patients had a lower complication rate when compared to no MPH hemostatic powder, 0.3% vs. 1.7% (p < .05), respectively.
29764229	7	13	theme	powder	1142:1147	arg1	patients					1158:1165	patients	1158:1165	patients	1158:1165	RESULTS The MPH hemostatic powder group of patients had a lower complication rate when compared to no MPH hemostatic powder, 0.3% vs. 1.7% (p < .05), respectively.
29764229	5	14	theme	patients	851:858	arg1	total					826:830	A total	824:830	A total of 283 consecutive patients	824:858	A total of 283 consecutive patients were retrospectively studied to assess the rate of complications in patients who underwent CIED implantation with MPH hemostatic powder (n = 77, MPH hemostatic powder) and without (n = 206, no MPH hemostatic powder).
29764229	5	15	dep	powder	989:994	arg1	n = 77					997:1002	n = 77	997:1002	n = 77	997:1002	A total of 283 consecutive patients were retrospectively studied to assess the rate of complications in patients who underwent CIED implantation with MPH hemostatic powder (n = 77, MPH hemostatic powder) and without (n = 206, no MPH hemostatic powder).
29764229	5	15	dep	powder	989:994	arg1	powder					1020:1025	MPH hemostatic powder	1005:1025	MPH hemostatic powder	1005:1025	A total of 283 consecutive patients were retrospectively studied to assess the rate of complications in patients who underwent CIED implantation with MPH hemostatic powder (n = 77, MPH hemostatic powder) and without (n = 206, no MPH hemostatic powder).
29764229	11	16	theme	infections	1862:1871	arg1	endpoint					1837:1844	a composite endpoint	1825:1844	a composite endpoint of hematoma and infections	1825:1871	CONCLUSION Using MPH hemostatic powder for post-procedural hemostasis was shown to result in a significant reduction in the rate of overall post-procedural complications (a composite endpoint of hematoma and infections), and a trend in reduction of the infections rates and device implantation site hematoma rates.
29764229	11	17	theme	trend	1881:1885	arg1	rate					1778:1781	the rate	1774:1781	the rate of overall post-procedural complications (a composite endpoint of hematoma and infections), and a trend in reduction of the infections rates and device implantation site hematoma rates	1774:1966	CONCLUSION Using MPH hemostatic powder for post-procedural hemostasis was shown to result in a significant reduction in the rate of overall post-procedural complications (a composite endpoint of hematoma and infections), and a trend in reduction of the infections rates and device implantation site hematoma rates.
29764229	4	18	theme	plant-derived	746:758	arg1	polysaccharide					772:785	a new plant-derived microporous polysaccharide hemostatic powder (Arista)	740:812	a new plant-derived microporous polysaccharide hemostatic powder (Arista)	740:812	METHODS In our study, a new plant-derived microporous polysaccharide hemostatic powder (Arista) was used.
29764229	4	18	theme	plant-derived	746:758	arg1	study					733:737	our study	729:737	our study	729:737	METHODS In our study, a new plant-derived microporous polysaccharide hemostatic powder (Arista) was used.
29764229	11	19	from	complications	1810:1822	arg1	reduction					1890:1898	reduction	1890:1898	reduction of the infections rates and device implantation site hematoma rates	1890:1966	CONCLUSION Using MPH hemostatic powder for post-procedural hemostasis was shown to result in a significant reduction in the rate of overall post-procedural complications (a composite endpoint of hematoma and infections), and a trend in reduction of the infections rates and device implantation site hematoma rates.
29764229	9	20	theme	postoperative	1420:1432	arg1	infections					1434:1443	no postoperative infections	1417:1443	no postoperative infections	1417:1443	There were no postoperative infections in the MPH hemostatic powder group versus 3.2% infections in the other group.
29764229	11	21	theme	hemostatic	1675:1684	arg1	powder					1686:1691	MPH hemostatic powder	1671:1691	MPH hemostatic powder for post-procedural hemostasis	1671:1722	CONCLUSION Using MPH hemostatic powder for post-procedural hemostasis was shown to result in a significant reduction in the rate of overall post-procedural complications (a composite endpoint of hematoma and infections), and a trend in reduction of the infections rates and device implantation site hematoma rates.
29764229	0	22	theme	postoperative	79:91	arg1	rates					93:97	postoperative rates	79:97	postoperative rates of pocket hematoma and infection	79:130	Use of MPH hemostatic powder for electrophysiology device implantation reduces postoperative rates of pocket hematoma and infection.
29764229	11	23	from	rate	1778:1781	arg1	reduction					1890:1898	reduction	1890:1898	reduction of the infections rates and device implantation site hematoma rates	1890:1966	CONCLUSION Using MPH hemostatic powder for post-procedural hemostasis was shown to result in a significant reduction in the rate of overall post-procedural complications (a composite endpoint of hematoma and infections), and a trend in reduction of the infections rates and device implantation site hematoma rates.
29764229	4	24	theme	new	742:744	arg1	polysaccharide					772:785	a new plant-derived microporous polysaccharide hemostatic powder (Arista)	740:812	a new plant-derived microporous polysaccharide hemostatic powder (Arista)	740:812	METHODS In our study, a new plant-derived microporous polysaccharide hemostatic powder (Arista) was used.
29764229	4	24	theme	new	742:744	arg1	study					733:737	our study	729:737	our study	729:737	METHODS In our study, a new plant-derived microporous polysaccharide hemostatic powder (Arista) was used.
29764229	7	25	theme	complication	1179:1190	arg1	%					1243:1243	0.3%	1240:1243	0.3%	1240:1243	RESULTS The MPH hemostatic powder group of patients had a lower complication rate when compared to no MPH hemostatic powder, 0.3% vs. 1.7% (p < .05), respectively.
29764229	7	25	theme	complication	1179:1190	arg1	%					1252:1252	1.7%	1249:1252	1.7%	1249:1252	RESULTS The MPH hemostatic powder group of patients had a lower complication rate when compared to no MPH hemostatic powder, 0.3% vs. 1.7% (p < .05), respectively.
29764229	7	25	theme	complication	1179:1190	arg1	rate					1192:1195	a lower complication rate	1171:1195	a lower complication rate when compared to no MPH hemostatic powder	1171:1237	RESULTS The MPH hemostatic powder group of patients had a lower complication rate when compared to no MPH hemostatic powder, 0.3% vs. 1.7% (p < .05), respectively.
29764229	11	26	theme	infections	1907:1916	arg1	rates					1918:1922	the infections rates	1903:1922	the infections rates	1903:1922	CONCLUSION Using MPH hemostatic powder for post-procedural hemostasis was shown to result in a significant reduction in the rate of overall post-procedural complications (a composite endpoint of hematoma and infections), and a trend in reduction of the infections rates and device implantation site hematoma rates.
29764229	9	27	theme	MPH	1452:1454	arg1	group					1474:1478	the MPH hemostatic powder group	1448:1478	the MPH hemostatic powder group	1448:1478	There were no postoperative infections in the MPH hemostatic powder group versus 3.2% infections in the other group.
29764229	7	28	dep	RESULTS	1115:1121	arg1	had					1167:1169	had	1167:1169	had	1167:1169	RESULTS The MPH hemostatic powder group of patients had a lower complication rate when compared to no MPH hemostatic powder, 0.3% vs. 1.7% (p < .05), respectively.
29764229	10	29	theme	complication	1579:1590	arg1	risk					1555:1558	increased risk	1545:1558	increased risk of post-procedural complication	1545:1590	The main predictor of increased risk of post-procedural complication was the usage of anticoagulation with a hazard ration of 2.7.
29764229	8	30	theme	device	1291:1296	arg1	hematoma					1320:1327	device implantation site MPH hematoma	1291:1327	device implantation site MPH hematoma in the MPH hemostatic powder group	1291:1362	The rate of device implantation site MPH hematoma in the MPH hemostatic powder group was 0.4%, versus 0.9% in the other group.
29764229	3	31	theme	study	436:440	arg1	aim					425:427	The aim	421:427	The aim of our study	421:440	OBJECTIVE The aim of our study is to compare the rate of complications in patients who underwent cardiac implantable electronic device (CIED) implantation utilizing MPH hemostatic powder to the rate of complications in those patients who underwent standard procedure protocol without MPH hemostatic powder.
29764229	0	32	theme	hematoma	109:116	arg1	rates					93:97	postoperative rates	79:97	postoperative rates of pocket hematoma and infection	79:130	Use of MPH hemostatic powder for electrophysiology device implantation reduces postoperative rates of pocket hematoma and infection.
29764229	9	33	theme	powder	1467:1472	arg1	group					1474:1478	the MPH hemostatic powder group	1448:1478	the MPH hemostatic powder group	1448:1478	There were no postoperative infections in the MPH hemostatic powder group versus 3.2% infections in the other group.
29764229	8	34	theme	site	1311:1314	arg1	hematoma					1320:1327	device implantation site MPH hematoma	1291:1327	device implantation site MPH hematoma in the MPH hemostatic powder group	1291:1362	The rate of device implantation site MPH hematoma in the MPH hemostatic powder group was 0.4%, versus 0.9% in the other group.
29764229	11	35	theme	post-procedural	1794:1808	arg1	complications					1810:1822	overall post-procedural complications	1786:1822	overall post-procedural complications (a composite endpoint of hematoma and infections)	1786:1872	CONCLUSION Using MPH hemostatic powder for post-procedural hemostasis was shown to result in a significant reduction in the rate of overall post-procedural complications (a composite endpoint of hematoma and infections), and a trend in reduction of the infections rates and device implantation site hematoma rates.
29764229	7	36	theme	patients	1158:1165	arg1	group					1149:1153	The MPH hemostatic powder group	1123:1153	The MPH hemostatic powder group of patients	1123:1165	RESULTS The MPH hemostatic powder group of patients had a lower complication rate when compared to no MPH hemostatic powder, 0.3% vs. 1.7% (p < .05), respectively.
29764229	7	36	theme	patients	1158:1165	arg1	patients					1158:1165	patients	1158:1165	patients	1158:1165	RESULTS The MPH hemostatic powder group of patients had a lower complication rate when compared to no MPH hemostatic powder, 0.3% vs. 1.7% (p < .05), respectively.
29764229	5	37	theme	CIED	951:954	arg1	implantation					956:967	CIED implantation	951:967	CIED implantation with MPH hemostatic powder (n = 77, MPH hemostatic powder) and without (n = 206, no MPH hemostatic powder)	951:1074	A total of 283 consecutive patients were retrospectively studied to assess the rate of complications in patients who underwent CIED implantation with MPH hemostatic powder (n = 77, MPH hemostatic powder) and without (n = 206, no MPH hemostatic powder).
29764229	8	38	theme	hematoma	1320:1327	arg1	%					1371:1371	0.4%	1368:1371	0.4%	1368:1371	The rate of device implantation site MPH hematoma in the MPH hemostatic powder group was 0.4%, versus 0.9% in the other group.
29764229	8	38	theme	hematoma	1320:1327	arg1	rate					1283:1286	The rate	1279:1286	The rate of device implantation site MPH hematoma in the MPH hemostatic powder group	1279:1362	The rate of device implantation site MPH hematoma in the MPH hemostatic powder group was 0.4%, versus 0.9% in the other group.
29764229	0	39	theme	MPH	7:9	arg1	powder					22:27	MPH hemostatic powder	7:27	MPH hemostatic powder	7:27	Use of MPH hemostatic powder for electrophysiology device implantation reduces postoperative rates of pocket hematoma and infection.
29764229	10	40	theme	2.7	1649:1651	arg1	ration					1639:1644	a hazard ration	1630:1644	a hazard ration of 2.7	1630:1651	The main predictor of increased risk of post-procedural complication was the usage of anticoagulation with a hazard ration of 2.7.
29764229	10	41	with	usage	1600:1604	arg1	ration					1639:1644	a hazard ration	1630:1644	a hazard ration of 2.7	1630:1651	The main predictor of increased risk of post-procedural complication was the usage of anticoagulation with a hazard ration of 2.7.
29764229	9	42	from	infections	1492:1501	arg1	group					1516:1520	the other group	1506:1520	the other group	1506:1520	There were no postoperative infections in the MPH hemostatic powder group versus 3.2% infections in the other group.
29764229	11	43	theme	composite	1827:1835	arg1	endpoint					1837:1844	a composite endpoint	1825:1844	a composite endpoint of hematoma and infections	1825:1871	CONCLUSION Using MPH hemostatic powder for post-procedural hemostasis was shown to result in a significant reduction in the rate of overall post-procedural complications (a composite endpoint of hematoma and infections), and a trend in reduction of the infections rates and device implantation site hematoma rates.
29764229	10	44	theme	post-procedural	1563:1577	arg1	complication					1579:1590	post-procedural complication	1563:1590	post-procedural complication	1563:1590	The main predictor of increased risk of post-procedural complication was the usage of anticoagulation with a hazard ration of 2.7.
29764229	0	45	theme	powder	22:27	arg1	Use					0:2	Use	0:2	Use of MPH hemostatic powder for electrophysiology device implantation	0:69	Use of MPH hemostatic powder for electrophysiology device implantation reduces postoperative rates of pocket hematoma and infection.
29764229	7	46	theme	hemostatic	1131:1140	arg1	group					1149:1153	The MPH hemostatic powder group	1123:1153	The MPH hemostatic powder group of patients	1123:1165	RESULTS The MPH hemostatic powder group of patients had a lower complication rate when compared to no MPH hemostatic powder, 0.3% vs. 1.7% (p < .05), respectively.
29764229	7	46	theme	hemostatic	1131:1140	arg1	patients					1158:1165	patients	1158:1165	patients	1158:1165	RESULTS The MPH hemostatic powder group of patients had a lower complication rate when compared to no MPH hemostatic powder, 0.3% vs. 1.7% (p < .05), respectively.
29764229	11	47	dep	complications	1810:1822	arg1	endpoint					1837:1844	a composite endpoint	1825:1844	a composite endpoint of hematoma and infections	1825:1871	CONCLUSION Using MPH hemostatic powder for post-procedural hemostasis was shown to result in a significant reduction in the rate of overall post-procedural complications (a composite endpoint of hematoma and infections), and a trend in reduction of the infections rates and device implantation site hematoma rates.
29764229	8	48	theme	hemostatic	1340:1349	arg1	group					1358:1362	the MPH hemostatic powder group	1332:1362	the MPH hemostatic powder group	1332:1362	The rate of device implantation site MPH hematoma in the MPH hemostatic powder group was 0.4%, versus 0.9% in the other group.
29764229	7	49	contain	had	1167:1169	arg1	group					1149:1153	The MPH hemostatic powder group	1123:1153	The MPH hemostatic powder group of patients	1123:1165	RESULTS The MPH hemostatic powder group of patients had a lower complication rate when compared to no MPH hemostatic powder, 0.3% vs. 1.7% (p < .05), respectively.
29764229	7	49	contain	had	1167:1169	arg2	%					1243:1243	0.3%	1240:1243	0.3%	1240:1243	RESULTS The MPH hemostatic powder group of patients had a lower complication rate when compared to no MPH hemostatic powder, 0.3% vs. 1.7% (p < .05), respectively.
29764229	7	49	contain	had	1167:1169	arg2	rate					1192:1195	a lower complication rate	1171:1195	a lower complication rate when compared to no MPH hemostatic powder	1171:1237	RESULTS The MPH hemostatic powder group of patients had a lower complication rate when compared to no MPH hemostatic powder, 0.3% vs. 1.7% (p < .05), respectively.
29764229	7	49	contain	had	1167:1169	arg2	%					1252:1252	1.7%	1249:1252	1.7%	1249:1252	RESULTS The MPH hemostatic powder group of patients had a lower complication rate when compared to no MPH hemostatic powder, 0.3% vs. 1.7% (p < .05), respectively.
29764229	7	49	contain	had	1167:1169	arg1	patients					1158:1165	patients	1158:1165	patients	1158:1165	RESULTS The MPH hemostatic powder group of patients had a lower complication rate when compared to no MPH hemostatic powder, 0.3% vs. 1.7% (p < .05), respectively.
29764229	10	50	theme	anticoagulation	1609:1623	arg1	predictor					1532:1540	The main predictor	1523:1540	The main predictor of increased risk of post-procedural complication	1523:1590	The main predictor of increased risk of post-procedural complication was the usage of anticoagulation with a hazard ration of 2.7.
29764229	10	50	theme	anticoagulation	1609:1623	arg1	usage					1600:1604	the usage	1596:1604	the usage of anticoagulation with a hazard ration of 2.7	1596:1651	The main predictor of increased risk of post-procedural complication was the usage of anticoagulation with a hazard ration of 2.7.
29764229	0	51	theme	electrophysiology	33:49	arg1	implantation					58:69	electrophysiology device implantation	33:69	electrophysiology device implantation	33:69	Use of MPH hemostatic powder for electrophysiology device implantation reduces postoperative rates of pocket hematoma and infection.
29764229	9	52	theme	other	1510:1514	arg1	group					1516:1520	the other group	1506:1520	the other group	1506:1520	There were no postoperative infections in the MPH hemostatic powder group versus 3.2% infections in the other group.
29764229	1	53	theme	device	238:243	arg1	procedures					258:267	electrophysiology (EP) device implantation procedures	215:267	electrophysiology (EP) device implantation procedures	215:267	BACKGROUND Surgical site bleeding and infection are potential complications after electrophysiology (EP) device implantation procedures.
29764229	3	54	theme	procedure	668:676	arg1	protocol					678:685	standard procedure protocol	659:685	standard procedure protocol	659:685	OBJECTIVE The aim of our study is to compare the rate of complications in patients who underwent cardiac implantable electronic device (CIED) implantation utilizing MPH hemostatic powder to the rate of complications in those patients who underwent standard procedure protocol without MPH hemostatic powder.
29764229	3	55	theme	cardiac	508:514	arg1	implantation					553:564	cardiac implantable electronic device (CIED) implantation	508:564	cardiac implantable electronic device (CIED) implantation utilizing MPH hemostatic powder to the rate of complications in those patients who underwent standard procedure protocol without MPH hemostatic powder	508:715	OBJECTIVE The aim of our study is to compare the rate of complications in patients who underwent cardiac implantable electronic device (CIED) implantation utilizing MPH hemostatic powder to the rate of complications in those patients who underwent standard procedure protocol without MPH hemostatic powder.
29764229	4	56	from	METHODS	718:724	arg1	polysaccharide					772:785	a new plant-derived microporous polysaccharide hemostatic powder (Arista)	740:812	a new plant-derived microporous polysaccharide hemostatic powder (Arista)	740:812	METHODS In our study, a new plant-derived microporous polysaccharide hemostatic powder (Arista) was used.
29764229	4	56	from	METHODS	718:724	arg1	study					733:737	our study	729:737	our study	729:737	METHODS In our study, a new plant-derived microporous polysaccharide hemostatic powder (Arista) was used.
29764229	5	57	theme	hemostatic	1009:1018	arg1	n = 77					997:1002	n = 77	997:1002	n = 77	997:1002	A total of 283 consecutive patients were retrospectively studied to assess the rate of complications in patients who underwent CIED implantation with MPH hemostatic powder (n = 77, MPH hemostatic powder) and without (n = 206, no MPH hemostatic powder).
29764229	5	57	theme	hemostatic	1009:1018	arg1	powder					1020:1025	MPH hemostatic powder	1005:1025	MPH hemostatic powder	1005:1025	A total of 283 consecutive patients were retrospectively studied to assess the rate of complications in patients who underwent CIED implantation with MPH hemostatic powder (n = 77, MPH hemostatic powder) and without (n = 206, no MPH hemostatic powder).
29764229	11	58	from	reduction	1890:1898	arg1	rate					1778:1781	the rate	1774:1781	the rate of overall post-procedural complications (a composite endpoint of hematoma and infections), and a trend in reduction of the infections rates and device implantation site hematoma rates	1774:1966	CONCLUSION Using MPH hemostatic powder for post-procedural hemostasis was shown to result in a significant reduction in the rate of overall post-procedural complications (a composite endpoint of hematoma and infections), and a trend in reduction of the infections rates and device implantation site hematoma rates.
29764229	7	59	theme	MPH	1217:1219	arg1	powder					1232:1237	no MPH hemostatic powder	1214:1237	no MPH hemostatic powder	1214:1237	RESULTS The MPH hemostatic powder group of patients had a lower complication rate when compared to no MPH hemostatic powder, 0.3% vs. 1.7% (p < .05), respectively.
29764229	3	60	theme	hemostatic	699:708	arg1	powder					710:715	MPH hemostatic powder	695:715	MPH hemostatic powder	695:715	OBJECTIVE The aim of our study is to compare the rate of complications in patients who underwent cardiac implantable electronic device (CIED) implantation utilizing MPH hemostatic powder to the rate of complications in those patients who underwent standard procedure protocol without MPH hemostatic powder.
29764229	11	61	theme	significant	1749:1759	arg1	reduction					1761:1769	a significant reduction	1747:1769	a significant reduction in the rate of overall post-procedural complications (a composite endpoint of hematoma and infections), and a trend in reduction of the infections rates and device implantation site hematoma rates	1747:1966	CONCLUSION Using MPH hemostatic powder for post-procedural hemostasis was shown to result in a significant reduction in the rate of overall post-procedural complications (a composite endpoint of hematoma and infections), and a trend in reduction of the infections rates and device implantation site hematoma rates.
29764229	3	62	theme	electronic	528:537	arg1	CIED					547:550	CIED	547:550	CIED	547:550	OBJECTIVE The aim of our study is to compare the rate of complications in patients who underwent cardiac implantable electronic device (CIED) implantation utilizing MPH hemostatic powder to the rate of complications in those patients who underwent standard procedure protocol without MPH hemostatic powder.
29764229	3	62	theme	electronic	528:537	arg1	device					539:544	implantable electronic device	516:544	cardiac implantable electronic device (CIED) implantation utilizing MPH hemostatic powder to the rate of complications in those patients who underwent standard procedure protocol without MPH hemostatic powder	508:715	OBJECTIVE The aim of our study is to compare the rate of complications in patients who underwent cardiac implantable electronic device (CIED) implantation utilizing MPH hemostatic powder to the rate of complications in those patients who underwent standard procedure protocol without MPH hemostatic powder.
29764229	11	63	from	trend	1881:1885	arg1	reduction					1890:1898	reduction	1890:1898	reduction of the infections rates and device implantation site hematoma rates	1890:1966	CONCLUSION Using MPH hemostatic powder for post-procedural hemostasis was shown to result in a significant reduction in the rate of overall post-procedural complications (a composite endpoint of hematoma and infections), and a trend in reduction of the infections rates and device implantation site hematoma rates.
29764229	5	64	with	implantation	956:967	arg1	powder					1068:1073	no MPH hemostatic powder	1050:1073	no MPH hemostatic powder	1050:1073	A total of 283 consecutive patients were retrospectively studied to assess the rate of complications in patients who underwent CIED implantation with MPH hemostatic powder (n = 77, MPH hemostatic powder) and without (n = 206, no MPH hemostatic powder).
29764229	5	64	with	implantation	956:967	arg1	powder					989:994	MPH hemostatic powder	974:994	MPH hemostatic powder (n = 77, MPH hemostatic powder)	974:1026	A total of 283 consecutive patients were retrospectively studied to assess the rate of complications in patients who underwent CIED implantation with MPH hemostatic powder (n = 77, MPH hemostatic powder) and without (n = 206, no MPH hemostatic powder).
29764229	5	64	with	implantation	956:967	arg1	n = 206					1041:1047	n = 206	1041:1047	n = 206	1041:1047	A total of 283 consecutive patients were retrospectively studied to assess the rate of complications in patients who underwent CIED implantation with MPH hemostatic powder (n = 77, MPH hemostatic powder) and without (n = 206, no MPH hemostatic powder).
29764229	8	65	from	%	1371:1371	arg1	group					1399:1403	the other group	1389:1403	the other group	1389:1403	The rate of device implantation site MPH hematoma in the MPH hemostatic powder group was 0.4%, versus 0.9% in the other group.
29764229	11	66	theme	device	1928:1933	arg1	rates					1962:1966	device implantation site hematoma rates	1928:1966	device implantation site hematoma rates	1928:1966	CONCLUSION Using MPH hemostatic powder for post-procedural hemostasis was shown to result in a significant reduction in the rate of overall post-procedural complications (a composite endpoint of hematoma and infections), and a trend in reduction of the infections rates and device implantation site hematoma rates.
29764229	2	67	theme	tools	306:310	arg1	tools					306:310	tools	306:310	tools for management of intraoperative bleeding	306:352	To date, there is a wide variety of tools for management of intraoperative bleeding but it still remains unclear what methods are preferred.
29764229	2	67	theme	tools	306:310	arg1	variety					295:301	a wide variety	288:301	a wide variety of tools for management of intraoperative bleeding	288:352	To date, there is a wide variety of tools for management of intraoperative bleeding but it still remains unclear what methods are preferred.
29764229	1	68	theme	electrophysiology	215:231	arg1	procedures					258:267	electrophysiology (EP) device implantation procedures	215:267	electrophysiology (EP) device implantation procedures	215:267	BACKGROUND Surgical site bleeding and infection are potential complications after electrophysiology (EP) device implantation procedures.
29764229	11	69	theme	implantation	1935:1946	arg1	rates					1962:1966	device implantation site hematoma rates	1928:1966	device implantation site hematoma rates	1928:1966	CONCLUSION Using MPH hemostatic powder for post-procedural hemostasis was shown to result in a significant reduction in the rate of overall post-procedural complications (a composite endpoint of hematoma and infections), and a trend in reduction of the infections rates and device implantation site hematoma rates.
29764229	10	70	theme	main	1527:1530	arg1	predictor					1532:1540	The main predictor	1523:1540	The main predictor of increased risk of post-procedural complication	1523:1590	The main predictor of increased risk of post-procedural complication was the usage of anticoagulation with a hazard ration of 2.7.
29764229	10	70	theme	main	1527:1530	arg1	usage					1600:1604	the usage	1596:1604	the usage of anticoagulation with a hazard ration of 2.7	1596:1651	The main predictor of increased risk of post-procedural complication was the usage of anticoagulation with a hazard ration of 2.7.
29764229	11	71	theme	post-procedural	1697:1711	arg1	hemostasis					1713:1722	post-procedural hemostasis	1697:1722	post-procedural hemostasis	1697:1722	CONCLUSION Using MPH hemostatic powder for post-procedural hemostasis was shown to result in a significant reduction in the rate of overall post-procedural complications (a composite endpoint of hematoma and infections), and a trend in reduction of the infections rates and device implantation site hematoma rates.
29764229	5	72	theme	hemostatic	1057:1066	arg1	powder					1068:1073	no MPH hemostatic powder	1050:1073	no MPH hemostatic powder	1050:1073	A total of 283 consecutive patients were retrospectively studied to assess the rate of complications in patients who underwent CIED implantation with MPH hemostatic powder (n = 77, MPH hemostatic powder) and without (n = 206, no MPH hemostatic powder).
29764229	5	72	theme	hemostatic	1057:1066	arg1	n = 206					1041:1047	n = 206	1041:1047	n = 206	1041:1047	A total of 283 consecutive patients were retrospectively studied to assess the rate of complications in patients who underwent CIED implantation with MPH hemostatic powder (n = 77, MPH hemostatic powder) and without (n = 206, no MPH hemostatic powder).
29764229	5	73	theme	consecutive	839:849	arg1	patients					851:858	283 consecutive patients	835:858	283 consecutive patients	835:858	A total of 283 consecutive patients were retrospectively studied to assess the rate of complications in patients who underwent CIED implantation with MPH hemostatic powder (n = 77, MPH hemostatic powder) and without (n = 206, no MPH hemostatic powder).
29764229	11	74	theme	hematoma	1953:1960	arg1	rates					1962:1966	device implantation site hematoma rates	1928:1966	device implantation site hematoma rates	1928:1966	CONCLUSION Using MPH hemostatic powder for post-procedural hemostasis was shown to result in a significant reduction in the rate of overall post-procedural complications (a composite endpoint of hematoma and infections), and a trend in reduction of the infections rates and device implantation site hematoma rates.
29764229	7	75	theme	lower	1173:1177	arg1	%					1243:1243	0.3%	1240:1243	0.3%	1240:1243	RESULTS The MPH hemostatic powder group of patients had a lower complication rate when compared to no MPH hemostatic powder, 0.3% vs. 1.7% (p < .05), respectively.
29764229	7	75	theme	lower	1173:1177	arg1	%					1252:1252	1.7%	1249:1252	1.7%	1249:1252	RESULTS The MPH hemostatic powder group of patients had a lower complication rate when compared to no MPH hemostatic powder, 0.3% vs. 1.7% (p < .05), respectively.
29764229	7	75	theme	lower	1173:1177	arg1	rate					1192:1195	a lower complication rate	1171:1195	a lower complication rate when compared to no MPH hemostatic powder	1171:1237	RESULTS The MPH hemostatic powder group of patients had a lower complication rate when compared to no MPH hemostatic powder, 0.3% vs. 1.7% (p < .05), respectively.
29764229	3	76	theme	hemostatic	580:589	arg1	powder					591:596	MPH hemostatic powder	576:596	MPH hemostatic powder	576:596	OBJECTIVE The aim of our study is to compare the rate of complications in patients who underwent cardiac implantable electronic device (CIED) implantation utilizing MPH hemostatic powder to the rate of complications in those patients who underwent standard procedure protocol without MPH hemostatic powder.
29764229	5	77	theme	complications	911:923	arg1	rate					903:906	the rate	899:906	the rate of complications	899:923	A total of 283 consecutive patients were retrospectively studied to assess the rate of complications in patients who underwent CIED implantation with MPH hemostatic powder (n = 77, MPH hemostatic powder) and without (n = 206, no MPH hemostatic powder).
29764229	10	78	theme	hazard	1632:1637	arg1	ration					1639:1644	a hazard ration	1630:1644	a hazard ration of 2.7	1630:1651	The main predictor of increased risk of post-procedural complication was the usage of anticoagulation with a hazard ration of 2.7.
29764229	3	79	theme	complications	613:625	arg1	rate					605:608	the rate	601:608	the rate of complications in those patients who underwent standard procedure protocol without MPH hemostatic powder	601:715	OBJECTIVE The aim of our study is to compare the rate of complications in patients who underwent cardiac implantable electronic device (CIED) implantation utilizing MPH hemostatic powder to the rate of complications in those patients who underwent standard procedure protocol without MPH hemostatic powder.
29764229	11	80	theme	rates	1918:1922	arg1	reduction					1890:1898	reduction	1890:1898	reduction of the infections rates and device implantation site hematoma rates	1890:1966	CONCLUSION Using MPH hemostatic powder for post-procedural hemostasis was shown to result in a significant reduction in the rate of overall post-procedural complications (a composite endpoint of hematoma and infections), and a trend in reduction of the infections rates and device implantation site hematoma rates.
29764229	8	81	theme	other	1393:1397	arg1	group					1399:1403	the other group	1389:1403	the other group	1389:1403	The rate of device implantation site MPH hematoma in the MPH hemostatic powder group was 0.4%, versus 0.9% in the other group.
29764229	11	82	theme	hematoma	1849:1856	arg1	endpoint					1837:1844	a composite endpoint	1825:1844	a composite endpoint of hematoma and infections	1825:1871	CONCLUSION Using MPH hemostatic powder for post-procedural hemostasis was shown to result in a significant reduction in the rate of overall post-procedural complications (a composite endpoint of hematoma and infections), and a trend in reduction of the infections rates and device implantation site hematoma rates.
29764229	9	83	dep	%	1490:1490	arg1	infections					1492:1501	infections	1492:1501	3.2% infections in the other group	1487:1520	There were no postoperative infections in the MPH hemostatic powder group versus 3.2% infections in the other group.
29764229	4	84	dep	polysaccharide	772:785	arg1	Arista					806:811	Arista	806:811	Arista	806:811	METHODS In our study, a new plant-derived microporous polysaccharide hemostatic powder (Arista) was used.
29764229	4	84	dep	polysaccharide	772:785	arg1	powder					798:803	hemostatic powder	787:803	a new plant-derived microporous polysaccharide hemostatic powder (Arista)	740:812	METHODS In our study, a new plant-derived microporous polysaccharide hemostatic powder (Arista) was used.
29764229	0	85	theme	pocket	102:107	arg1	hematoma					109:116	pocket hematoma	102:116	pocket hematoma	102:116	Use of MPH hemostatic powder for electrophysiology device implantation reduces postoperative rates of pocket hematoma and infection.
29764229	5	86	theme	MPH	974:976	arg1	powder					989:994	MPH hemostatic powder	974:994	MPH hemostatic powder (n = 77, MPH hemostatic powder)	974:1026	A total of 283 consecutive patients were retrospectively studied to assess the rate of complications in patients who underwent CIED implantation with MPH hemostatic powder (n = 77, MPH hemostatic powder) and without (n = 206, no MPH hemostatic powder).
29764229	5	87	theme	hemostatic	978:987	arg1	powder					989:994	MPH hemostatic powder	974:994	MPH hemostatic powder (n = 77, MPH hemostatic powder)	974:1026	A total of 283 consecutive patients were retrospectively studied to assess the rate of complications in patients who underwent CIED implantation with MPH hemostatic powder (n = 77, MPH hemostatic powder) and without (n = 206, no MPH hemostatic powder).
29764229	4	88	theme	microporous	760:770	arg1	polysaccharide					772:785	a new plant-derived microporous polysaccharide hemostatic powder (Arista)	740:812	a new plant-derived microporous polysaccharide hemostatic powder (Arista)	740:812	METHODS In our study, a new plant-derived microporous polysaccharide hemostatic powder (Arista) was used.
29764229	4	88	theme	microporous	760:770	arg1	study					733:737	our study	729:737	our study	729:737	METHODS In our study, a new plant-derived microporous polysaccharide hemostatic powder (Arista) was used.
29764229	3	89	from	rate	605:608	arg1	patients					636:643	those patients	630:643	those patients who underwent standard procedure protocol without MPH hemostatic powder	630:715	OBJECTIVE The aim of our study is to compare the rate of complications in patients who underwent cardiac implantable electronic device (CIED) implantation utilizing MPH hemostatic powder to the rate of complications in those patients who underwent standard procedure protocol without MPH hemostatic powder.
29764229	11	90	theme	MPH	1671:1673	arg1	powder					1686:1691	MPH hemostatic powder	1671:1691	MPH hemostatic powder for post-procedural hemostasis	1671:1722	CONCLUSION Using MPH hemostatic powder for post-procedural hemostasis was shown to result in a significant reduction in the rate of overall post-procedural complications (a composite endpoint of hematoma and infections), and a trend in reduction of the infections rates and device implantation site hematoma rates.
29764229	9	91	theme	hemostatic	1456:1465	arg1	group					1474:1478	the MPH hemostatic powder group	1448:1478	the MPH hemostatic powder group	1448:1478	There were no postoperative infections in the MPH hemostatic powder group versus 3.2% infections in the other group.
29764229	3	92	dep	OBJECTIVE	411:419	arg1	is					442:443	is	442:443	is to compare the rate of complications in patients who underwent cardiac implantable electronic device (CIED) implantation utilizing MPH hemostatic powder to the rate of complications in those patients who underwent standard procedure protocol without MPH hemostatic powder	442:715	OBJECTIVE The aim of our study is to compare the rate of complications in patients who underwent cardiac implantable electronic device (CIED) implantation utilizing MPH hemostatic powder to the rate of complications in those patients who underwent standard procedure protocol without MPH hemostatic powder.
29764229	4	93	theme	hemostatic	787:796	arg1	Arista					806:811	Arista	806:811	Arista	806:811	METHODS In our study, a new plant-derived microporous polysaccharide hemostatic powder (Arista) was used.
29764229	4	93	theme	hemostatic	787:796	arg1	powder					798:803	hemostatic powder	787:803	a new plant-derived microporous polysaccharide hemostatic powder (Arista)	740:812	METHODS In our study, a new plant-derived microporous polysaccharide hemostatic powder (Arista) was used.
29764229	3	94	from	complications	613:625	arg1	patients					636:643	those patients	630:643	those patients who underwent standard procedure protocol without MPH hemostatic powder	630:715	OBJECTIVE The aim of our study is to compare the rate of complications in patients who underwent cardiac implantable electronic device (CIED) implantation utilizing MPH hemostatic powder to the rate of complications in those patients who underwent standard procedure protocol without MPH hemostatic powder.
29764229	10	95	theme	increased	1545:1553	arg1	risk					1555:1558	increased risk	1545:1558	increased risk of post-procedural complication	1545:1590	The main predictor of increased risk of post-procedural complication was the usage of anticoagulation with a hazard ration of 2.7.
29764229	11	96	from	reduction	1761:1769	arg1	rate					1778:1781	the rate	1774:1781	the rate of overall post-procedural complications (a composite endpoint of hematoma and infections), and a trend in reduction of the infections rates and device implantation site hematoma rates	1774:1966	CONCLUSION Using MPH hemostatic powder for post-procedural hemostasis was shown to result in a significant reduction in the rate of overall post-procedural complications (a composite endpoint of hematoma and infections), and a trend in reduction of the infections rates and device implantation site hematoma rates.
29764229	8	97	theme	implantation	1298:1309	arg1	hematoma					1320:1327	device implantation site MPH hematoma	1291:1327	device implantation site MPH hematoma in the MPH hemostatic powder group	1291:1362	The rate of device implantation site MPH hematoma in the MPH hemostatic powder group was 0.4%, versus 0.9% in the other group.
29764229	11	98	theme	complications	1810:1822	arg1	rate					1778:1781	the rate	1774:1781	the rate of overall post-procedural complications (a composite endpoint of hematoma and infections), and a trend in reduction of the infections rates and device implantation site hematoma rates	1774:1966	CONCLUSION Using MPH hemostatic powder for post-procedural hemostasis was shown to result in a significant reduction in the rate of overall post-procedural complications (a composite endpoint of hematoma and infections), and a trend in reduction of the infections rates and device implantation site hematoma rates.
29764229	0	99	theme	hemostatic	11:20	arg1	powder					22:27	MPH hemostatic powder	7:27	MPH hemostatic powder	7:27	Use of MPH hemostatic powder for electrophysiology device implantation reduces postoperative rates of pocket hematoma and infection.
29764229	8	100	theme	MPH	1316:1318	arg1	hematoma					1320:1327	device implantation site MPH hematoma	1291:1327	device implantation site MPH hematoma in the MPH hemostatic powder group	1291:1362	The rate of device implantation site MPH hematoma in the MPH hemostatic powder group was 0.4%, versus 0.9% in the other group.
29764229	4	101	link	plant-derived	746:758	arg1	polysaccharide					772:785	a new plant-derived microporous polysaccharide hemostatic powder (Arista)	740:812	a new plant-derived microporous polysaccharide hemostatic powder (Arista)	740:812	METHODS In our study, a new plant-derived microporous polysaccharide hemostatic powder (Arista) was used.
29764229	4	101	link	plant-derived	746:758	arg1	study					733:737	our study	729:737	our study	729:737	METHODS In our study, a new plant-derived microporous polysaccharide hemostatic powder (Arista) was used.
29764229	7	102	theme	MPH	1127:1129	arg1	group					1149:1153	The MPH hemostatic powder group	1123:1153	The MPH hemostatic powder group of patients	1123:1165	RESULTS The MPH hemostatic powder group of patients had a lower complication rate when compared to no MPH hemostatic powder, 0.3% vs. 1.7% (p < .05), respectively.
29764229	7	102	theme	MPH	1127:1129	arg1	patients					1158:1165	patients	1158:1165	patients	1158:1165	RESULTS The MPH hemostatic powder group of patients had a lower complication rate when compared to no MPH hemostatic powder, 0.3% vs. 1.7% (p < .05), respectively.
29764229	10	103	theme	risk	1555:1558	arg1	predictor					1532:1540	The main predictor	1523:1540	The main predictor of increased risk of post-procedural complication	1523:1590	The main predictor of increased risk of post-procedural complication was the usage of anticoagulation with a hazard ration of 2.7.
29764229	10	103	theme	risk	1555:1558	arg1	usage					1600:1604	the usage	1596:1604	the usage of anticoagulation with a hazard ration of 2.7	1596:1651	The main predictor of increased risk of post-procedural complication was the usage of anticoagulation with a hazard ration of 2.7.
29764229	0	104	theme	device	51:56	arg1	implantation					58:69	electrophysiology device implantation	33:69	electrophysiology device implantation	33:69	Use of MPH hemostatic powder for electrophysiology device implantation reduces postoperative rates of pocket hematoma and infection.
29764229	8	105	theme	MPH	1336:1338	arg1	group					1358:1362	the MPH hemostatic powder group	1332:1362	the MPH hemostatic powder group	1332:1362	The rate of device implantation site MPH hematoma in the MPH hemostatic powder group was 0.4%, versus 0.9% in the other group.
29764229	2	106	theme	bleeding	345:352	arg1	management					316:325	management	316:325	management of intraoperative bleeding	316:352	To date, there is a wide variety of tools for management of intraoperative bleeding but it still remains unclear what methods are preferred.
29764229	3	107	theme	standard	659:666	arg1	protocol					678:685	standard procedure protocol	659:685	standard procedure protocol	659:685	OBJECTIVE The aim of our study is to compare the rate of complications in patients who underwent cardiac implantable electronic device (CIED) implantation utilizing MPH hemostatic powder to the rate of complications in those patients who underwent standard procedure protocol without MPH hemostatic powder.
29764229	8	108	theme	powder	1351:1356	arg1	group					1358:1362	the MPH hemostatic powder group	1332:1362	the MPH hemostatic powder group	1332:1362	The rate of device implantation site MPH hematoma in the MPH hemostatic powder group was 0.4%, versus 0.9% in the other group.
29764229	3	109	theme	complications	468:480	arg1	rate					460:463	the rate	456:463	the rate of complications	456:480	OBJECTIVE The aim of our study is to compare the rate of complications in patients who underwent cardiac implantable electronic device (CIED) implantation utilizing MPH hemostatic powder to the rate of complications in those patients who underwent standard procedure protocol without MPH hemostatic powder.
29764229	8	110	from	rate	1283:1286	arg1	group					1358:1362	the MPH hemostatic powder group	1332:1362	the MPH hemostatic powder group	1332:1362	The rate of device implantation site MPH hematoma in the MPH hemostatic powder group was 0.4%, versus 0.9% in the other group.
29764229	1	111	theme	implantation	245:256	arg1	procedures					258:267	electrophysiology (EP) device implantation procedures	215:267	electrophysiology (EP) device implantation procedures	215:267	BACKGROUND Surgical site bleeding and infection are potential complications after electrophysiology (EP) device implantation procedures.
29764229	5	112	theme	MPH	1005:1007	arg1	n = 77					997:1002	n = 77	997:1002	n = 77	997:1002	A total of 283 consecutive patients were retrospectively studied to assess the rate of complications in patients who underwent CIED implantation with MPH hemostatic powder (n = 77, MPH hemostatic powder) and without (n = 206, no MPH hemostatic powder).
29764229	5	112	theme	MPH	1005:1007	arg1	powder					1020:1025	MPH hemostatic powder	1005:1025	MPH hemostatic powder	1005:1025	A total of 283 consecutive patients were retrospectively studied to assess the rate of complications in patients who underwent CIED implantation with MPH hemostatic powder (n = 77, MPH hemostatic powder) and without (n = 206, no MPH hemostatic powder).
29764229	7	113	theme	hemostatic	1221:1230	arg1	powder					1232:1237	no MPH hemostatic powder	1214:1237	no MPH hemostatic powder	1214:1237	RESULTS The MPH hemostatic powder group of patients had a lower complication rate when compared to no MPH hemostatic powder, 0.3% vs. 1.7% (p < .05), respectively.
29764229	3	114	theme	implantable	516:526	arg1	CIED					547:550	CIED	547:550	CIED	547:550	OBJECTIVE The aim of our study is to compare the rate of complications in patients who underwent cardiac implantable electronic device (CIED) implantation utilizing MPH hemostatic powder to the rate of complications in those patients who underwent standard procedure protocol without MPH hemostatic powder.
29764229	3	114	theme	implantable	516:526	arg1	device					539:544	implantable electronic device	516:544	cardiac implantable electronic device (CIED) implantation utilizing MPH hemostatic powder to the rate of complications in those patients who underwent standard procedure protocol without MPH hemostatic powder	508:715	OBJECTIVE The aim of our study is to compare the rate of complications in patients who underwent cardiac implantable electronic device (CIED) implantation utilizing MPH hemostatic powder to the rate of complications in those patients who underwent standard procedure protocol without MPH hemostatic powder.
29764229	8	115	from	hematoma	1320:1327	arg1	group					1358:1362	the MPH hemostatic powder group	1332:1362	the MPH hemostatic powder group	1332:1362	The rate of device implantation site MPH hematoma in the MPH hemostatic powder group was 0.4%, versus 0.9% in the other group.
30259170	4	0	theme	controlled	519:528	arg1	conditions					530:539	controlled conditions	519:539	controlled conditions	519:539	half a year from seeds at ambient control (525 ppm), 750, 900, and 1000 ppm atmospheric pCO2 under controlled conditions.
30259170	5	1	theme	wall	605:608	arg1	composition					610:620	the cell wall composition	596:620	the cell wall composition of the	596:627	Increasing pCO2 enhanced biomass production, modified the cell wall composition of the leaves in favor of cellulose at the expense of lignin, and enhanced the foliar non-structural carbohydrate level, in particular the sucrose content; as well as total N content of leaves by increased levels of all major N fractions, i.e., soluble proteins, total amino acids, and structural N.
30259170	1	2	theme	lignin	238:243	arg1	expense					227:233	the expense	223:233	the expense of lignin	223:243	Atmospheric p CO 2 impacts Quercus petraea biomass production and cell wall composition of the leaves in favor of cellulose at the expense of lignin, and enhances foliar non-structural carbohydrate levels and sucrose contents in a pCO 2 concentration-dependent manner.
30259170	5	3	theme	carbohydrate	723:734	arg1	level					736:740	the foliar non-structural carbohydrate level	697:740	the foliar non-structural carbohydrate level	697:740	Increasing pCO2 enhanced biomass production, modified the cell wall composition of the leaves in favor of cellulose at the expense of lignin, and enhanced the foliar non-structural carbohydrate level, in particular the sucrose content; as well as total N content of leaves by increased levels of all major N fractions, i.e., soluble proteins, total amino acids, and structural N.
30259170	0	4	theme	Atmospheric	0:10	arg1	pCO2					12:15	Atmospheric pCO2	0:15	Atmospheric pCO2	0:15	Atmospheric pCO2 impacts leaf structural and physiological traits in Quercus petraea seedlings.
30259170	5	5	theme	total	789:793	arg1	content					797:803	total N content	789:803	total N content of leaves by increased levels of all major N fractions, i.e., soluble proteins, total amino acids, and structural N	789:919	Increasing pCO2 enhanced biomass production, modified the cell wall composition of the leaves in favor of cellulose at the expense of lignin, and enhanced the foliar non-structural carbohydrate level, in particular the sucrose content; as well as total N content of leaves by increased levels of all major N fractions, i.e., soluble proteins, total amino acids, and structural N.
30259170	8	6	theme	reductase	1153:1161	arg1	activity					1163:1170	High in vitro glutathione reductase activity	1127:1170	High in vitro glutathione reductase activity at reduced H2O2 contents	1127:1195	High in vitro glutathione reductase activity at reduced H2O2 contents suggests enhanced ROS scavenging, but increased lipid peroxidation may also have contributed, as indicated by a negative correlation between malone dialdehyde and H2O2 contents.
30259170	10	7	theme	atmospheric	1500:1510	arg1	pCO2					1512:1515	atmospheric pCO2	1500:1515	atmospheric pCO2	1500:1515	Apparently, the interaction of atmospheric pCO2 with leaf structural and physiological traits of Q. petraea seedlings is characterized by a dynamic response depending on the pCO2 level.
30259170	4	8	from	seeds	437:441	arg1	year					427:430	year	427:430	year	427:430	half a year from seeds at ambient control (525 ppm), 750, 900, and 1000 ppm atmospheric pCO2 under controlled conditions.
30259170	1	9	theme	the	187:189	arg1	petraea					131:137	Quercus petraea	123:137	Quercus petraea	123:137	Atmospheric p CO 2 impacts Quercus petraea biomass production and cell wall composition of the leaves in favor of cellulose at the expense of lignin, and enhances foliar non-structural carbohydrate levels and sucrose contents in a pCO 2 concentration-dependent manner.
30259170	1	9	theme	the	187:189	arg1	composition					172:182	cell wall composition	162:182	cell wall composition	162:182	Atmospheric p CO 2 impacts Quercus petraea biomass production and cell wall composition of the leaves in favor of cellulose at the expense of lignin, and enhances foliar non-structural carbohydrate levels and sucrose contents in a pCO 2 concentration-dependent manner.
30259170	5	10	theme	fractions	850:858	arg1	levels					828:833	increased levels	818:833	increased levels of all major N fractions, i.e., soluble proteins, total amino acids, and structural N	818:919	Increasing pCO2 enhanced biomass production, modified the cell wall composition of the leaves in favor of cellulose at the expense of lignin, and enhanced the foliar non-structural carbohydrate level, in particular the sucrose content; as well as total N content of leaves by increased levels of all major N fractions, i.e., soluble proteins, total amino acids, and structural N.
30259170	8	11	theme	H2O2	1183:1186	arg1	contents					1188:1195	reduced H2O2 contents	1175:1195	reduced H2O2 contents	1175:1195	High in vitro glutathione reductase activity at reduced H2O2 contents suggests enhanced ROS scavenging, but increased lipid peroxidation may also have contributed, as indicated by a negative correlation between malone dialdehyde and H2O2 contents.
30259170	10	12	theme	structural	1527:1536	arg1	traits					1556:1561	leaf structural and physiological traits	1522:1561	leaf structural and physiological traits of Q. petraea seedlings	1522:1585	Apparently, the interaction of atmospheric pCO2 with leaf structural and physiological traits of Q. petraea seedlings is characterized by a dynamic response depending on the pCO2 level.
30259170	4	13	dep	year	427:430	arg1	a					425:425	a	425:425	a	425:425	half a year from seeds at ambient control (525 ppm), 750, 900, and 1000 ppm atmospheric pCO2 under controlled conditions.
30259170	5	14	from	expense	665:671	arg1	cellulose					648:656	cellulose	648:656	cellulose at the expense of lignin	648:681	Increasing pCO2 enhanced biomass production, modified the cell wall composition of the leaves in favor of cellulose at the expense of lignin, and enhanced the foliar non-structural carbohydrate level, in particular the sucrose content; as well as total N content of leaves by increased levels of all major N fractions, i.e., soluble proteins, total amino acids, and structural N.
30259170	8	15	theme	High	1127:1130	arg1	activity					1163:1170	High in vitro glutathione reductase activity	1127:1170	High in vitro glutathione reductase activity at reduced H2O2 contents	1127:1195	High in vitro glutathione reductase activity at reduced H2O2 contents suggests enhanced ROS scavenging, but increased lipid peroxidation may also have contributed, as indicated by a negative correlation between malone dialdehyde and H2O2 contents.
30259170	5	16	dep	proteins	875:882	arg1	i.e.					861:864	i.e.	861:864	i.e.	861:864	Increasing pCO2 enhanced biomass production, modified the cell wall composition of the leaves in favor of cellulose at the expense of lignin, and enhanced the foliar non-structural carbohydrate level, in particular the sucrose content; as well as total N content of leaves by increased levels of all major N fractions, i.e., soluble proteins, total amino acids, and structural N.
30259170	8	17	theme	ROS	1215:1217	arg1	scavenging					1219:1228	enhanced ROS scavenging	1206:1228	enhanced ROS scavenging	1206:1228	High in vitro glutathione reductase activity at reduced H2O2 contents suggests enhanced ROS scavenging, but increased lipid peroxidation may also have contributed, as indicated by a negative correlation between malone dialdehyde and H2O2 contents.
30259170	5	18	theme	major	842:846	arg1	fractions					850:858	all major N fractions	838:858	all major N fractions	838:858	Increasing pCO2 enhanced biomass production, modified the cell wall composition of the leaves in favor of cellulose at the expense of lignin, and enhanced the foliar non-structural carbohydrate level, in particular the sucrose content; as well as total N content of leaves by increased levels of all major N fractions, i.e., soluble proteins, total amino acids, and structural N.
30259170	8	19	theme	increased	1235:1243	arg1	peroxidation					1251:1262	increased lipid peroxidation	1235:1262	increased lipid peroxidation	1235:1262	High in vitro glutathione reductase activity at reduced H2O2 contents suggests enhanced ROS scavenging, but increased lipid peroxidation may also have contributed, as indicated by a negative correlation between malone dialdehyde and H2O2 contents.
30259170	0	20	theme	petraea	77:83	arg1	seedlings					85:93	Quercus petraea seedlings	69:93	Quercus petraea seedlings	69:93	Atmospheric pCO2 impacts leaf structural and physiological traits in Quercus petraea seedlings.
30259170	1	21	theme	pCO	327:329	arg1	manner					357:362	a pCO 2 concentration-dependent manner	325:362	a pCO 2 concentration-dependent manner	325:362	Atmospheric p CO 2 impacts Quercus petraea biomass production and cell wall composition of the leaves in favor of cellulose at the expense of lignin, and enhances foliar non-structural carbohydrate levels and sucrose contents in a pCO 2 concentration-dependent manner.
30259170	5	22	theme	Increasing	542:551	arg1	pCO2					553:556	Increasing pCO2	542:556	Increasing pCO2	542:556	Increasing pCO2 enhanced biomass production, modified the cell wall composition of the leaves in favor of cellulose at the expense of lignin, and enhanced the foliar non-structural carbohydrate level, in particular the sucrose content; as well as total N content of leaves by increased levels of all major N fractions, i.e., soluble proteins, total amino acids, and structural N.
30259170	1	23	theme	cell	162:165	arg1	composition					172:182	cell wall composition	162:182	cell wall composition	162:182	Atmospheric p CO 2 impacts Quercus petraea biomass production and cell wall composition of the leaves in favor of cellulose at the expense of lignin, and enhances foliar non-structural carbohydrate levels and sucrose contents in a pCO 2 concentration-dependent manner.
30259170	7	24	dep	the	1077:1079	arg1	indicated					1091:1099	indicated	1091:1099	indicated by reduced H2O2 contents	1091:1124	Increasing pCO2 alleviated oxidative stress in the leaves as indicated by reduced H2O2 contents.
30259170	7	24	dep	the	1077:1079	arg1	leaves					1081:1086	leaves	1081:1086	leaves	1081:1086	Increasing pCO2 alleviated oxidative stress in the leaves as indicated by reduced H2O2 contents.
30259170	7	25	theme	reduced	1104:1110	arg1	contents					1117:1124	reduced H2O2 contents	1104:1124	reduced H2O2 contents	1104:1124	Increasing pCO2 alleviated oxidative stress in the leaves as indicated by reduced H2O2 contents.
30259170	1	26	theme	concentration-dependent	333:355	arg1	manner					357:362	a pCO 2 concentration-dependent manner	325:362	a pCO 2 concentration-dependent manner	325:362	Atmospheric p CO 2 impacts Quercus petraea biomass production and cell wall composition of the leaves in favor of cellulose at the expense of lignin, and enhances foliar non-structural carbohydrate levels and sucrose contents in a pCO 2 concentration-dependent manner.
30259170	5	27	theme	soluble	867:873	arg1	proteins					875:882	soluble proteins	867:882	soluble proteins	867:882	Increasing pCO2 enhanced biomass production, modified the cell wall composition of the leaves in favor of cellulose at the expense of lignin, and enhanced the foliar non-structural carbohydrate level, in particular the sucrose content; as well as total N content of leaves by increased levels of all major N fractions, i.e., soluble proteins, total amino acids, and structural N.
30259170	2	28	theme	petraea	386:392	arg1	oak					373:375	Sessile oak	365:375	Sessile oak (Quercus petraea Liebl.)	365:400	Sessile oak (Quercus petraea Liebl.)
30259170	2	28	theme	petraea	386:392	arg1	Liebl					394:398	Quercus petraea Liebl.	378:399	Quercus petraea Liebl.	378:399	Sessile oak (Quercus petraea Liebl.)
30259170	4	29	theme	ambient	446:452	arg1	525 ppm					463:469	525 ppm	463:469	525 ppm	463:469	half a year from seeds at ambient control (525 ppm), 750, 900, and 1000 ppm atmospheric pCO2 under controlled conditions.
30259170	4	29	theme	ambient	446:452	arg1	control					454:460	ambient control	446:460	ambient control (525 ppm)	446:470	half a year from seeds at ambient control (525 ppm), 750, 900, and 1000 ppm atmospheric pCO2 under controlled conditions.
30259170	5	30	theme	particular	746:755	arg1	content					769:775	the sucrose content	757:775	particular the sucrose content; as well as total N content of leaves by increased levels of all major N fractions, i.e., soluble proteins, total amino acids, and structural N	746:919	Increasing pCO2 enhanced biomass production, modified the cell wall composition of the leaves in favor of cellulose at the expense of lignin, and enhanced the foliar non-structural carbohydrate level, in particular the sucrose content; as well as total N content of leaves by increased levels of all major N fractions, i.e., soluble proteins, total amino acids, and structural N.
30259170	1	31	theme	Atmospheric	96:106	arg1	CO					110:111	Atmospheric p CO 2	96:113	Atmospheric p CO 2	96:113	Atmospheric p CO 2 impacts Quercus petraea biomass production and cell wall composition of the leaves in favor of cellulose at the expense of lignin, and enhances foliar non-structural carbohydrate levels and sucrose contents in a pCO 2 concentration-dependent manner.
30259170	6	32	theme	acid	947:950	arg1	level					952:956	The enhanced total amino acid level	922:956	The enhanced total amino acid level	922:956	The enhanced total amino acid level was largely due to 2-ketoglutarate and oxalo acetate-derived compounds.
30259170	6	32	theme	acid	947:950	arg1	due					970:972	due	970:972	due	970:972	The enhanced total amino acid level was largely due to 2-ketoglutarate and oxalo acetate-derived compounds.
30259170	5	33	theme	sucrose	761:767	arg1	content					769:775	the sucrose content	757:775	particular the sucrose content; as well as total N content of leaves by increased levels of all major N fractions, i.e., soluble proteins, total amino acids, and structural N	746:919	Increasing pCO2 enhanced biomass production, modified the cell wall composition of the leaves in favor of cellulose at the expense of lignin, and enhanced the foliar non-structural carbohydrate level, in particular the sucrose content; as well as total N content of leaves by increased levels of all major N fractions, i.e., soluble proteins, total amino acids, and structural N.
30259170	1	34	theme	non-structural	266:279	arg1	levels					294:299	foliar non-structural carbohydrate levels	259:299	foliar non-structural carbohydrate levels	259:299	Atmospheric p CO 2 impacts Quercus petraea biomass production and cell wall composition of the leaves in favor of cellulose at the expense of lignin, and enhances foliar non-structural carbohydrate levels and sucrose contents in a pCO 2 concentration-dependent manner.
30259170	8	35	dep	in	1132:1133	arg1	vitro					1135:1139	vitro	1135:1139	vitro	1135:1139	High in vitro glutathione reductase activity at reduced H2O2 contents suggests enhanced ROS scavenging, but increased lipid peroxidation may also have contributed, as indicated by a negative correlation between malone dialdehyde and H2O2 contents.
30259170	6	36	theme	total	935:939	arg1	level					952:956	The enhanced total amino acid level	922:956	The enhanced total amino acid level	922:956	The enhanced total amino acid level was largely due to 2-ketoglutarate and oxalo acetate-derived compounds.
30259170	6	36	theme	total	935:939	arg1	due					970:972	due	970:972	due	970:972	The enhanced total amino acid level was largely due to 2-ketoglutarate and oxalo acetate-derived compounds.
30259170	4	37	theme	atmospheric	496:506	arg1	pCO2					508:511	ambient control (525 ppm), 750, 900, and 1000 ppm atmospheric pCO2	446:511	ambient control (525 ppm), 750, 900, and 1000 ppm atmospheric pCO2	446:511	half a year from seeds at ambient control (525 ppm), 750, 900, and 1000 ppm atmospheric pCO2 under controlled conditions.
30259170	10	38	theme	seedlings	1577:1585	arg1	traits					1556:1561	leaf structural and physiological traits	1522:1561	leaf structural and physiological traits of Q. petraea seedlings	1522:1585	Apparently, the interaction of atmospheric pCO2 with leaf structural and physiological traits of Q. petraea seedlings is characterized by a dynamic response depending on the pCO2 level.
30259170	7	39	theme	oxidative	1057:1065	arg1	stress					1067:1072	oxidative stress	1057:1072	oxidative stress	1057:1072	Increasing pCO2 alleviated oxidative stress in the leaves as indicated by reduced H2O2 contents.
30259170	0	40	theme	structural	30:39	arg1	traits					59:64	leaf structural and physiological traits	25:64	leaf structural and physiological traits in Quercus petraea seedlings	25:93	Atmospheric pCO2 impacts leaf structural and physiological traits in Quercus petraea seedlings.
30259170	5	41	theme	structural	908:917	arg1	N					919:919	structural N	908:919	structural N	908:919	Increasing pCO2 enhanced biomass production, modified the cell wall composition of the leaves in favor of cellulose at the expense of lignin, and enhanced the foliar non-structural carbohydrate level, in particular the sucrose content; as well as total N content of leaves by increased levels of all major N fractions, i.e., soluble proteins, total amino acids, and structural N.
30259170	4	42	theme	750	473:475	arg1	525 ppm					463:469	525 ppm	463:469	525 ppm	463:469	half a year from seeds at ambient control (525 ppm), 750, 900, and 1000 ppm atmospheric pCO2 under controlled conditions.
30259170	4	42	theme	750	473:475	arg1	control					454:460	ambient control	446:460	ambient control (525 ppm)	446:470	half a year from seeds at ambient control (525 ppm), 750, 900, and 1000 ppm atmospheric pCO2 under controlled conditions.
30259170	8	43	theme	negative	1309:1316	arg1	correlation					1318:1328	a negative correlation	1307:1328	a negative correlation between malone dialdehyde and H2O2 contents	1307:1372	High in vitro glutathione reductase activity at reduced H2O2 contents suggests enhanced ROS scavenging, but increased lipid peroxidation may also have contributed, as indicated by a negative correlation between malone dialdehyde and H2O2 contents.
30259170	0	44	theme	physiological	45:57	arg1	traits					59:64	leaf structural and physiological traits	25:64	leaf structural and physiological traits in Quercus petraea seedlings	25:93	Atmospheric pCO2 impacts leaf structural and physiological traits in Quercus petraea seedlings.
30259170	1	45	theme	sucrose	305:311	arg1	contents					313:320	sucrose contents	305:320	sucrose contents	305:320	Atmospheric p CO 2 impacts Quercus petraea biomass production and cell wall composition of the leaves in favor of cellulose at the expense of lignin, and enhances foliar non-structural carbohydrate levels and sucrose contents in a pCO 2 concentration-dependent manner.
30259170	4	46	theme	900	478:480	arg1	525 ppm					463:469	525 ppm	463:469	525 ppm	463:469	half a year from seeds at ambient control (525 ppm), 750, 900, and 1000 ppm atmospheric pCO2 under controlled conditions.
30259170	4	46	theme	900	478:480	arg1	control					454:460	ambient control	446:460	ambient control (525 ppm)	446:470	half a year from seeds at ambient control (525 ppm), 750, 900, and 1000 ppm atmospheric pCO2 under controlled conditions.
30259170	5	47	theme	amino	891:895	arg1	acids					897:901	total amino acids	885:901	total amino acids	885:901	Increasing pCO2 enhanced biomass production, modified the cell wall composition of the leaves in favor of cellulose at the expense of lignin, and enhanced the foliar non-structural carbohydrate level, in particular the sucrose content; as well as total N content of leaves by increased levels of all major N fractions, i.e., soluble proteins, total amino acids, and structural N.
30259170	6	48	theme	oxalo	997:1001	arg1	compounds					1019:1027	2-ketoglutarate and oxalo acetate-derived compounds	977:1027	2-ketoglutarate and oxalo acetate-derived compounds	977:1027	The enhanced total amino acid level was largely due to 2-ketoglutarate and oxalo acetate-derived compounds.
30259170	2	49	theme	Sessile	365:371	arg1	oak					373:375	Sessile oak	365:375	Sessile oak (Quercus petraea Liebl.)	365:400	Sessile oak (Quercus petraea Liebl.)
30259170	2	49	theme	Sessile	365:371	arg1	Liebl					394:398	Quercus petraea Liebl.	378:399	Quercus petraea Liebl.	378:399	Sessile oak (Quercus petraea Liebl.)
30259170	6	50	theme	2-ketoglutarate	977:991	arg1	compounds					1019:1027	2-ketoglutarate and oxalo acetate-derived compounds	977:1027	2-ketoglutarate and oxalo acetate-derived compounds	977:1027	The enhanced total amino acid level was largely due to 2-ketoglutarate and oxalo acetate-derived compounds.
30259170	5	51	theme	cell	600:603	arg1	composition					610:620	the cell wall composition	596:620	the cell wall composition of the	596:627	Increasing pCO2 enhanced biomass production, modified the cell wall composition of the leaves in favor of cellulose at the expense of lignin, and enhanced the foliar non-structural carbohydrate level, in particular the sucrose content; as well as total N content of leaves by increased levels of all major N fractions, i.e., soluble proteins, total amino acids, and structural N.
30259170	1	52	theme	wall	167:170	arg1	composition					172:182	cell wall composition	162:182	cell wall composition	162:182	Atmospheric p CO 2 impacts Quercus petraea biomass production and cell wall composition of the leaves in favor of cellulose at the expense of lignin, and enhances foliar non-structural carbohydrate levels and sucrose contents in a pCO 2 concentration-dependent manner.
30259170	8	53	theme	in	1132:1133	arg1	activity					1163:1170	High in vitro glutathione reductase activity	1127:1170	High in vitro glutathione reductase activity at reduced H2O2 contents	1127:1195	High in vitro glutathione reductase activity at reduced H2O2 contents suggests enhanced ROS scavenging, but increased lipid peroxidation may also have contributed, as indicated by a negative correlation between malone dialdehyde and H2O2 contents.
30259170	10	54	theme	pCO2	1512:1515	arg1	interaction					1485:1495	the interaction	1481:1495	the interaction of atmospheric pCO2 with leaf structural and physiological traits of Q. petraea seedlings	1481:1585	Apparently, the interaction of atmospheric pCO2 with leaf structural and physiological traits of Q. petraea seedlings is characterized by a dynamic response depending on the pCO2 level.
30259170	5	55	theme	foliar	701:706	arg1	level					736:740	the foliar non-structural carbohydrate level	697:740	the foliar non-structural carbohydrate level	697:740	Increasing pCO2 enhanced biomass production, modified the cell wall composition of the leaves in favor of cellulose at the expense of lignin, and enhanced the foliar non-structural carbohydrate level, in particular the sucrose content; as well as total N content of leaves by increased levels of all major N fractions, i.e., soluble proteins, total amino acids, and structural N.
30259170	5	56	theme	N	795:795	arg1	content					797:803	total N content	789:803	total N content of leaves by increased levels of all major N fractions, i.e., soluble proteins, total amino acids, and structural N	789:919	Increasing pCO2 enhanced biomass production, modified the cell wall composition of the leaves in favor of cellulose at the expense of lignin, and enhanced the foliar non-structural carbohydrate level, in particular the sucrose content; as well as total N content of leaves by increased levels of all major N fractions, i.e., soluble proteins, total amino acids, and structural N.
30259170	8	57	theme	glutathione	1141:1151	arg1	activity					1163:1170	High in vitro glutathione reductase activity	1127:1170	High in vitro glutathione reductase activity at reduced H2O2 contents	1127:1195	High in vitro glutathione reductase activity at reduced H2O2 contents suggests enhanced ROS scavenging, but increased lipid peroxidation may also have contributed, as indicated by a negative correlation between malone dialdehyde and H2O2 contents.
30259170	8	58	theme	H2O2	1360:1363	arg1	contents					1365:1372	H2O2 contents	1360:1372	H2O2 contents	1360:1372	High in vitro glutathione reductase activity at reduced H2O2 contents suggests enhanced ROS scavenging, but increased lipid peroxidation may also have contributed, as indicated by a negative correlation between malone dialdehyde and H2O2 contents.
30259170	5	59	theme	increased	818:826	arg1	levels					828:833	increased levels	818:833	increased levels of all major N fractions, i.e., soluble proteins, total amino acids, and structural N	818:919	Increasing pCO2 enhanced biomass production, modified the cell wall composition of the leaves in favor of cellulose at the expense of lignin, and enhanced the foliar non-structural carbohydrate level, in particular the sucrose content; as well as total N content of leaves by increased levels of all major N fractions, i.e., soluble proteins, total amino acids, and structural N.
30259170	8	60	theme	reduced	1175:1181	arg1	contents					1188:1195	reduced H2O2 contents	1175:1195	reduced H2O2 contents	1175:1195	High in vitro glutathione reductase activity at reduced H2O2 contents suggests enhanced ROS scavenging, but increased lipid peroxidation may also have contributed, as indicated by a negative correlation between malone dialdehyde and H2O2 contents.
30259170	10	61	theme	leaf	1522:1525	arg1	traits					1556:1561	leaf structural and physiological traits	1522:1561	leaf structural and physiological traits of Q. petraea seedlings	1522:1585	Apparently, the interaction of atmospheric pCO2 with leaf structural and physiological traits of Q. petraea seedlings is characterized by a dynamic response depending on the pCO2 level.
30259170	5	62	theme	the	625:627	arg1	composition					610:620	the cell wall composition	596:620	the cell wall composition of the	596:627	Increasing pCO2 enhanced biomass production, modified the cell wall composition of the leaves in favor of cellulose at the expense of lignin, and enhanced the foliar non-structural carbohydrate level, in particular the sucrose content; as well as total N content of leaves by increased levels of all major N fractions, i.e., soluble proteins, total amino acids, and structural N.
30259170	1	63	theme	Quercus	123:129	arg1	petraea					131:137	Quercus petraea	123:137	Quercus petraea	123:137	Atmospheric p CO 2 impacts Quercus petraea biomass production and cell wall composition of the leaves in favor of cellulose at the expense of lignin, and enhances foliar non-structural carbohydrate levels and sucrose contents in a pCO 2 concentration-dependent manner.
30259170	8	64	theme	enhanced	1206:1213	arg1	scavenging					1219:1228	enhanced ROS scavenging	1206:1228	enhanced ROS scavenging	1206:1228	High in vitro glutathione reductase activity at reduced H2O2 contents suggests enhanced ROS scavenging, but increased lipid peroxidation may also have contributed, as indicated by a negative correlation between malone dialdehyde and H2O2 contents.
30259170	0	65	theme	Quercus	69:75	arg1	petraea					77:83	Quercus petraea	69:83	Quercus petraea seedlings	69:93	Atmospheric pCO2 impacts leaf structural and physiological traits in Quercus petraea seedlings.
30259170	1	66	theme	biomass	139:145	arg1	production					147:156	biomass production	139:156	biomass production	139:156	Atmospheric p CO 2 impacts Quercus petraea biomass production and cell wall composition of the leaves in favor of cellulose at the expense of lignin, and enhances foliar non-structural carbohydrate levels and sucrose contents in a pCO 2 concentration-dependent manner.
30259170	7	67	theme	H2O2	1112:1115	arg1	contents					1117:1124	reduced H2O2 contents	1104:1124	reduced H2O2 contents	1104:1124	Increasing pCO2 alleviated oxidative stress in the leaves as indicated by reduced H2O2 contents.
30259170	1	68	from	composition	172:182	arg1	favor					201:205	favor	201:205	favor	201:205	Atmospheric p CO 2 impacts Quercus petraea biomass production and cell wall composition of the leaves in favor of cellulose at the expense of lignin, and enhances foliar non-structural carbohydrate levels and sucrose contents in a pCO 2 concentration-dependent manner.
30259170	0	69	from	traits	59:64	arg1	seedlings					85:93	Quercus petraea seedlings	69:93	Quercus petraea seedlings	69:93	Atmospheric pCO2 impacts leaf structural and physiological traits in Quercus petraea seedlings.
30259170	8	70	theme	lipid	1245:1249	arg1	peroxidation					1251:1262	increased lipid peroxidation	1235:1262	increased lipid peroxidation	1235:1262	High in vitro glutathione reductase activity at reduced H2O2 contents suggests enhanced ROS scavenging, but increased lipid peroxidation may also have contributed, as indicated by a negative correlation between malone dialdehyde and H2O2 contents.
30259170	1	71	dep	petraea	131:137	arg1	production					147:156	biomass production	139:156	biomass production	139:156	Atmospheric p CO 2 impacts Quercus petraea biomass production and cell wall composition of the leaves in favor of cellulose at the expense of lignin, and enhances foliar non-structural carbohydrate levels and sucrose contents in a pCO 2 concentration-dependent manner.
30259170	5	72	theme	lignin	676:681	arg1	expense					665:671	the expense	661:671	the expense of lignin	661:681	Increasing pCO2 enhanced biomass production, modified the cell wall composition of the leaves in favor of cellulose at the expense of lignin, and enhanced the foliar non-structural carbohydrate level, in particular the sucrose content; as well as total N content of leaves by increased levels of all major N fractions, i.e., soluble proteins, total amino acids, and structural N.
30259170	5	73	theme	biomass	567:573	arg1	production					575:584	biomass production	567:584	biomass production	567:584	Increasing pCO2 enhanced biomass production, modified the cell wall composition of the leaves in favor of cellulose at the expense of lignin, and enhanced the foliar non-structural carbohydrate level, in particular the sucrose content; as well as total N content of leaves by increased levels of all major N fractions, i.e., soluble proteins, total amino acids, and structural N.
30259170	2	74	theme	Quercus	378:384	arg1	oak					373:375	Sessile oak	365:375	Sessile oak (Quercus petraea Liebl.)	365:400	Sessile oak (Quercus petraea Liebl.)
30259170	2	74	theme	Quercus	378:384	arg1	Liebl					394:398	Quercus petraea Liebl.	378:399	Quercus petraea Liebl.	378:399	Sessile oak (Quercus petraea Liebl.)
30259170	4	75	from	pCO2	508:511	arg1	seeds					437:441	seeds	437:441	seeds at ambient control (525 ppm), 750, 900, and 1000 ppm atmospheric pCO2	437:511	half a year from seeds at ambient control (525 ppm), 750, 900, and 1000 ppm atmospheric pCO2 under controlled conditions.
30259170	1	76	from	petraea	131:137	arg1	favor					201:205	favor	201:205	favor	201:205	Atmospheric p CO 2 impacts Quercus petraea biomass production and cell wall composition of the leaves in favor of cellulose at the expense of lignin, and enhances foliar non-structural carbohydrate levels and sucrose contents in a pCO 2 concentration-dependent manner.
30259170	5	77	theme	N	848:848	arg1	fractions					850:858	all major N fractions	838:858	all major N fractions	838:858	Increasing pCO2 enhanced biomass production, modified the cell wall composition of the leaves in favor of cellulose at the expense of lignin, and enhanced the foliar non-structural carbohydrate level, in particular the sucrose content; as well as total N content of leaves by increased levels of all major N fractions, i.e., soluble proteins, total amino acids, and structural N.
30259170	10	78	theme	physiological	1542:1554	arg1	traits					1556:1561	leaf structural and physiological traits	1522:1561	leaf structural and physiological traits of Q. petraea seedlings	1522:1585	Apparently, the interaction of atmospheric pCO2 with leaf structural and physiological traits of Q. petraea seedlings is characterized by a dynamic response depending on the pCO2 level.
30259170	0	79	theme	leaf	25:28	arg1	traits					59:64	leaf structural and physiological traits	25:64	leaf structural and physiological traits in Quercus petraea seedlings	25:93	Atmospheric pCO2 impacts leaf structural and physiological traits in Quercus petraea seedlings.
30259170	1	80	theme	foliar	259:264	arg1	levels					294:299	foliar non-structural carbohydrate levels	259:299	foliar non-structural carbohydrate levels	259:299	Atmospheric p CO 2 impacts Quercus petraea biomass production and cell wall composition of the leaves in favor of cellulose at the expense of lignin, and enhances foliar non-structural carbohydrate levels and sucrose contents in a pCO 2 concentration-dependent manner.
30259170	8	81	from	contents	1188:1195	arg1	activity					1163:1170	High in vitro glutathione reductase activity	1127:1170	High in vitro glutathione reductase activity at reduced H2O2 contents	1127:1195	High in vitro glutathione reductase activity at reduced H2O2 contents suggests enhanced ROS scavenging, but increased lipid peroxidation may also have contributed, as indicated by a negative correlation between malone dialdehyde and H2O2 contents.
30259170	1	82	theme	p	108:108	arg1	CO					110:111	Atmospheric p CO 2	96:113	Atmospheric p CO 2	96:113	Atmospheric p CO 2 impacts Quercus petraea biomass production and cell wall composition of the leaves in favor of cellulose at the expense of lignin, and enhances foliar non-structural carbohydrate levels and sucrose contents in a pCO 2 concentration-dependent manner.
30259170	6	83	theme	amino	941:945	arg1	level					952:956	The enhanced total amino acid level	922:956	The enhanced total amino acid level	922:956	The enhanced total amino acid level was largely due to 2-ketoglutarate and oxalo acetate-derived compounds.
30259170	6	83	theme	amino	941:945	arg1	due					970:972	due	970:972	due	970:972	The enhanced total amino acid level was largely due to 2-ketoglutarate and oxalo acetate-derived compounds.
30259170	6	84	link	acetate-derived	1003:1017	arg1	compounds					1019:1027	2-ketoglutarate and oxalo acetate-derived compounds	977:1027	2-ketoglutarate and oxalo acetate-derived compounds	977:1027	The enhanced total amino acid level was largely due to 2-ketoglutarate and oxalo acetate-derived compounds.
30259170	10	85	theme	pCO2	1643:1646	arg1	level					1648:1652	the pCO2 level	1639:1652	the pCO2 level	1639:1652	Apparently, the interaction of atmospheric pCO2 with leaf structural and physiological traits of Q. petraea seedlings is characterized by a dynamic response depending on the pCO2 level.
30259170	5	86	theme	non-structural	708:721	arg1	level					736:740	the foliar non-structural carbohydrate level	697:740	the foliar non-structural carbohydrate level	697:740	Increasing pCO2 enhanced biomass production, modified the cell wall composition of the leaves in favor of cellulose at the expense of lignin, and enhanced the foliar non-structural carbohydrate level, in particular the sucrose content; as well as total N content of leaves by increased levels of all major N fractions, i.e., soluble proteins, total amino acids, and structural N.
30259170	1	87	theme	carbohydrate	281:292	arg1	levels					294:299	foliar non-structural carbohydrate levels	259:299	foliar non-structural carbohydrate levels	259:299	Atmospheric p CO 2 impacts Quercus petraea biomass production and cell wall composition of the leaves in favor of cellulose at the expense of lignin, and enhances foliar non-structural carbohydrate levels and sucrose contents in a pCO 2 concentration-dependent manner.
30259170	6	88	theme	enhanced	926:933	arg1	level					952:956	The enhanced total amino acid level	922:956	The enhanced total amino acid level	922:956	The enhanced total amino acid level was largely due to 2-ketoglutarate and oxalo acetate-derived compounds.
30259170	6	88	theme	enhanced	926:933	arg1	due					970:972	due	970:972	due	970:972	The enhanced total amino acid level was largely due to 2-ketoglutarate and oxalo acetate-derived compounds.
30259170	10	89	theme	Q.	1566:1567	arg1	petraea					1569:1575	Q. petraea	1566:1575	Q. petraea seedlings	1566:1585	Apparently, the interaction of atmospheric pCO2 with leaf structural and physiological traits of Q. petraea seedlings is characterized by a dynamic response depending on the pCO2 level.
30259170	1	90	dep	the	187:189	arg1	leaves					191:196	leaves	191:196	leaves	191:196	Atmospheric p CO 2 impacts Quercus petraea biomass production and cell wall composition of the leaves in favor of cellulose at the expense of lignin, and enhances foliar non-structural carbohydrate levels and sucrose contents in a pCO 2 concentration-dependent manner.
30259170	10	91	theme	petraea	1569:1575	arg1	seedlings					1577:1585	Q. petraea seedlings	1566:1585	Q. petraea seedlings	1566:1585	Apparently, the interaction of atmospheric pCO2 with leaf structural and physiological traits of Q. petraea seedlings is characterized by a dynamic response depending on the pCO2 level.
30259170	7	92	theme	Increasing	1030:1039	arg1	pCO2					1041:1044	Increasing pCO2	1030:1044	Increasing pCO2	1030:1044	Increasing pCO2 alleviated oxidative stress in the leaves as indicated by reduced H2O2 contents.
30259170	10	93	with	interaction	1485:1495	arg1	traits					1556:1561	leaf structural and physiological traits	1522:1561	leaf structural and physiological traits of Q. petraea seedlings	1522:1585	Apparently, the interaction of atmospheric pCO2 with leaf structural and physiological traits of Q. petraea seedlings is characterized by a dynamic response depending on the pCO2 level.
30259170	10	94	theme	dynamic	1609:1615	arg1	response					1617:1624	a dynamic response	1607:1624	a dynamic response depending on the pCO2 level	1607:1652	Apparently, the interaction of atmospheric pCO2 with leaf structural and physiological traits of Q. petraea seedlings is characterized by a dynamic response depending on the pCO2 level.
30259170	5	95	theme	total	885:889	arg1	acids					897:901	total amino acids	885:901	total amino acids	885:901	Increasing pCO2 enhanced biomass production, modified the cell wall composition of the leaves in favor of cellulose at the expense of lignin, and enhanced the foliar non-structural carbohydrate level, in particular the sucrose content; as well as total N content of leaves by increased levels of all major N fractions, i.e., soluble proteins, total amino acids, and structural N.
30259170	6	96	theme	acetate-derived	1003:1017	arg1	compounds					1019:1027	2-ketoglutarate and oxalo acetate-derived compounds	977:1027	2-ketoglutarate and oxalo acetate-derived compounds	977:1027	The enhanced total amino acid level was largely due to 2-ketoglutarate and oxalo acetate-derived compounds.
30259170	8	97	theme	malone	1338:1343	arg1	dialdehyde					1345:1354	malone dialdehyde	1338:1354	malone dialdehyde	1338:1354	High in vitro glutathione reductase activity at reduced H2O2 contents suggests enhanced ROS scavenging, but increased lipid peroxidation may also have contributed, as indicated by a negative correlation between malone dialdehyde and H2O2 contents.
29300153	8	0	theme	name	1410:1413	arg1	sp					1441:1442	the name Bifidobacterium catulorum sp	1406:1442	the name Bifidobacterium catulorum sp	1406:1442	Based on the phylogenetic, genotypic and phenotypic data reported, strain MRM 8.19T represents a novel taxon within the genus Bifidobacterium for which the name Bifidobacterium catulorum sp.
29300153	2	1	theme	baby	323:326	arg1	marmosets					335:343	five baby common marmosets	318:343	five baby common marmosets	318:343	In our previous study based on hsp60 PCR-restriction fragment length polymorphism and 16S rRNA gene sequencing, we stated that the bifidobacterial strains isolated from the individual faecal samples of five baby common marmosets constituted different phylogenetically isolated groups of the genus Bifidobacterium.
29300153	1	2	from	faeces	59:64	arg1	taxon					44:48	a novel taxon	36:48	a novel taxon from the faeces of the baby common marmoset (Callithrix jacchus)	36:113	nov., a novel taxon from the faeces of the baby common marmoset (Callithrix jacchus).
29300153	1	2	from	faeces	59:64	arg1	nov.					30:33	nov.	30:33	nov.	30:33	nov., a novel taxon from the faeces of the baby common marmoset (Callithrix jacchus).
29300153	6	3	theme	rRNA	986:989	arg1	Analysis					970:977	Analysis	970:977	Analysis of 16S rRNA and hsp60 gene sequences	970:1014	Analysis of 16S rRNA and hsp60 gene sequences revealed that strain MRM 8.19T was related to B. tissieri DSM 100201T (95.8 %) and to Bifidobacterium bifidum ATCC 29521T (93.7 %), respectively.
29300153	6	4	dep	rRNA	986:989	arg1	sequences					1006:1014	gene sequences	1001:1014	gene sequences	1001:1014	Analysis of 16S rRNA and hsp60 gene sequences revealed that strain MRM 8.19T was related to B. tissieri DSM 100201T (95.8 %) and to Bifidobacterium bifidum ATCC 29521T (93.7 %), respectively.
29300153	5	5	theme	rRNA	915:918	arg1	analysis					891:898	analysis	891:898	analysis of partial 16S rRNA, hsp60, clpC, dnaJ, dnaG and rpoB gene sequences	891:967	Another strain, designated MRM 8.19T, has been classified as member of the genus Bifidobacterium on the basis of positive results for fructose-6-phosphate phosphoketolase activity and analysis of partial 16S rRNA, hsp60, clpC, dnaJ, dnaG and rpoB gene sequences.
29300153	5	5	theme	rRNA	915:918	arg1	activity					878:885	fructose-6-phosphate phosphoketolase activity	841:885	fructose-6-phosphate phosphoketolase activity	841:885	Another strain, designated MRM 8.19T, has been classified as member of the genus Bifidobacterium on the basis of positive results for fructose-6-phosphate phosphoketolase activity and analysis of partial 16S rRNA, hsp60, clpC, dnaJ, dnaG and rpoB gene sequences.
29300153	6	6	theme	B.	1062:1063	arg1	100201T					1078:1084	B. tissieri DSM 100201T	1062:1084	B. tissieri DSM 100201T (95.8 %)	1062:1093	Analysis of 16S rRNA and hsp60 gene sequences revealed that strain MRM 8.19T was related to B. tissieri DSM 100201T (95.8 %) and to Bifidobacterium bifidum ATCC 29521T (93.7 %), respectively.
29300153	6	6	theme	B.	1062:1063	arg1	%					1092:1092	95.8 %	1087:1092	95.8 %	1087:1092	Analysis of 16S rRNA and hsp60 gene sequences revealed that strain MRM 8.19T was related to B. tissieri DSM 100201T (95.8 %) and to Bifidobacterium bifidum ATCC 29521T (93.7 %), respectively.
29300153	6	7	theme	16S	982:984	arg1	rRNA					986:989	16S rRNA	982:989	16S rRNA	982:989	Analysis of 16S rRNA and hsp60 gene sequences revealed that strain MRM 8.19T was related to B. tissieri DSM 100201T (95.8 %) and to Bifidobacterium bifidum ATCC 29521T (93.7 %), respectively.
29300153	8	8	theme	catulorum	1431:1439	arg1	sp					1441:1442	the name Bifidobacterium catulorum sp	1406:1442	the name Bifidobacterium catulorum sp	1406:1442	Based on the phylogenetic, genotypic and phenotypic data reported, strain MRM 8.19T represents a novel taxon within the genus Bifidobacterium for which the name Bifidobacterium catulorum sp.
29300153	2	9	theme	bifidobacterial	247:261	arg1	strains					263:269	the bifidobacterial strains	243:269	the bifidobacterial strains isolated from the individual faecal samples of five baby common marmosets	243:343	In our previous study based on hsp60 PCR-restriction fragment length polymorphism and 16S rRNA gene sequencing, we stated that the bifidobacterial strains isolated from the individual faecal samples of five baby common marmosets constituted different phylogenetically isolated groups of the genus Bifidobacterium.
29300153	8	10	theme	phenotypic	1295:1304	arg1	data					1306:1309	the phylogenetic, genotypic and phenotypic data	1263:1309	data	1306:1309	Based on the phylogenetic, genotypic and phenotypic data reported, strain MRM 8.19T represents a novel taxon within the genus Bifidobacterium for which the name Bifidobacterium catulorum sp.
29300153	9	11	theme	103154T=JCM	1477:1487	arg1	31794T					1489:1494	type strain MRM 8.19T=DSM 103154T=JCM 31794T	1451:1494	type strain MRM 8.19T=DSM 103154T=JCM 31794T	1451:1494	nov. (type strain MRM 8.19T=DSM 103154T=JCM 31794T) is proposed.
29300153	9	11	theme	103154T=JCM	1477:1487	arg1	nov					1445:1447	nov	1445:1447	nov	1445:1447	nov. (type strain MRM 8.19T=DSM 103154T=JCM 31794T) is proposed.
29300153	5	12	theme	gene	954:957	arg1	sequences					959:967	rpoB gene sequences	949:967	rpoB gene sequences	949:967	Another strain, designated MRM 8.19T, has been classified as member of the genus Bifidobacterium on the basis of positive results for fructose-6-phosphate phosphoketolase activity and analysis of partial 16S rRNA, hsp60, clpC, dnaJ, dnaG and rpoB gene sequences.
29300153	3	13	theme	genus	534:538	arg1	Bifidobacterium					540:554	the genus Bifidobacterium	530:554	the genus Bifidobacterium	530:554	In that study, we also proposed that these isolated groups potentially represented novel species of the genus Bifidobacterium.
29300153	5	14	theme	hsp60	921:925	arg1	analysis					891:898	analysis	891:898	analysis of partial 16S rRNA, hsp60, clpC, dnaJ, dnaG and rpoB gene sequences	891:967	Another strain, designated MRM 8.19T, has been classified as member of the genus Bifidobacterium on the basis of positive results for fructose-6-phosphate phosphoketolase activity and analysis of partial 16S rRNA, hsp60, clpC, dnaJ, dnaG and rpoB gene sequences.
29300153	5	14	theme	hsp60	921:925	arg1	activity					878:885	fructose-6-phosphate phosphoketolase activity	841:885	fructose-6-phosphate phosphoketolase activity	841:885	Another strain, designated MRM 8.19T, has been classified as member of the genus Bifidobacterium on the basis of positive results for fructose-6-phosphate phosphoketolase activity and analysis of partial 16S rRNA, hsp60, clpC, dnaJ, dnaG and rpoB gene sequences.
29300153	6	15	theme	strain	1030:1035	arg1	8.19T					1041:1045	strain MRM 8.19T	1030:1045	strain MRM 8.19T	1030:1045	Analysis of 16S rRNA and hsp60 gene sequences revealed that strain MRM 8.19T was related to B. tissieri DSM 100201T (95.8 %) and to Bifidobacterium bifidum ATCC 29521T (93.7 %), respectively.
29300153	1	16	dep	marmoset	85:92	arg1	jacchus					106:112	Callithrix jacchus	95:112	Callithrix jacchus	95:112	nov., a novel taxon from the faeces of the baby common marmoset (Callithrix jacchus).
29300153	6	17	dep	bifidum	1118:1124	arg1	29521T					1131:1136	ATCC 29521T	1126:1136	Bifidobacterium bifidum ATCC 29521T (93.7 %)	1102:1145	Analysis of 16S rRNA and hsp60 gene sequences revealed that strain MRM 8.19T was related to B. tissieri DSM 100201T (95.8 %) and to Bifidobacterium bifidum ATCC 29521T (93.7 %), respectively.
29300153	2	18	theme	isolated	384:391	arg1	Bifidobacterium					413:427	the genus Bifidobacterium	403:427	the genus Bifidobacterium	403:427	In our previous study based on hsp60 PCR-restriction fragment length polymorphism and 16S rRNA gene sequencing, we stated that the bifidobacterial strains isolated from the individual faecal samples of five baby common marmosets constituted different phylogenetically isolated groups of the genus Bifidobacterium.
29300153	2	18	theme	isolated	384:391	arg1	groups					393:398	different phylogenetically isolated groups	357:398	different phylogenetically isolated groups of the genus Bifidobacterium	357:427	In our previous study based on hsp60 PCR-restriction fragment length polymorphism and 16S rRNA gene sequencing, we stated that the bifidobacterial strains isolated from the individual faecal samples of five baby common marmosets constituted different phylogenetically isolated groups of the genus Bifidobacterium.
29300153	6	19	theme	ATCC	1126:1129	arg1	29521T					1131:1136	ATCC 29521T	1126:1136	Bifidobacterium bifidum ATCC 29521T (93.7 %)	1102:1145	Analysis of 16S rRNA and hsp60 gene sequences revealed that strain MRM 8.19T was related to B. tissieri DSM 100201T (95.8 %) and to Bifidobacterium bifidum ATCC 29521T (93.7 %), respectively.
29300153	0	20	theme	catulorum	16:24	arg1	sp					26:27	Bifidobacterium catulorum sp	0:27	Bifidobacterium catulorum sp.	0:28	Bifidobacterium catulorum sp.
29300153	3	21	theme	novel	513:517	arg1	species					519:525	novel species	513:525	novel species of the genus Bifidobacterium	513:554	In that study, we also proposed that these isolated groups potentially represented novel species of the genus Bifidobacterium.
29300153	9	22	theme	8.19T=DSM	1467:1475	arg1	31794T					1489:1494	type strain MRM 8.19T=DSM 103154T=JCM 31794T	1451:1494	type strain MRM 8.19T=DSM 103154T=JCM 31794T	1451:1494	nov. (type strain MRM 8.19T=DSM 103154T=JCM 31794T) is proposed.
29300153	9	22	theme	8.19T=DSM	1467:1475	arg1	nov					1445:1447	nov	1445:1447	nov	1445:1447	nov. (type strain MRM 8.19T=DSM 103154T=JCM 31794T) is proposed.
29300153	5	23	theme	dnaJ	934:937	arg1	analysis					891:898	analysis	891:898	analysis of partial 16S rRNA, hsp60, clpC, dnaJ, dnaG and rpoB gene sequences	891:967	Another strain, designated MRM 8.19T, has been classified as member of the genus Bifidobacterium on the basis of positive results for fructose-6-phosphate phosphoketolase activity and analysis of partial 16S rRNA, hsp60, clpC, dnaJ, dnaG and rpoB gene sequences.
29300153	5	23	theme	dnaJ	934:937	arg1	activity					878:885	fructose-6-phosphate phosphoketolase activity	841:885	fructose-6-phosphate phosphoketolase activity	841:885	Another strain, designated MRM 8.19T, has been classified as member of the genus Bifidobacterium on the basis of positive results for fructose-6-phosphate phosphoketolase activity and analysis of partial 16S rRNA, hsp60, clpC, dnaJ, dnaG and rpoB gene sequences.
29300153	2	24	theme	different	357:365	arg1	Bifidobacterium					413:427	the genus Bifidobacterium	403:427	the genus Bifidobacterium	403:427	In our previous study based on hsp60 PCR-restriction fragment length polymorphism and 16S rRNA gene sequencing, we stated that the bifidobacterial strains isolated from the individual faecal samples of five baby common marmosets constituted different phylogenetically isolated groups of the genus Bifidobacterium.
29300153	2	24	theme	different	357:365	arg1	groups					393:398	different phylogenetically isolated groups	357:398	different phylogenetically isolated groups of the genus Bifidobacterium	357:427	In our previous study based on hsp60 PCR-restriction fragment length polymorphism and 16S rRNA gene sequencing, we stated that the bifidobacterial strains isolated from the individual faecal samples of five baby common marmosets constituted different phylogenetically isolated groups of the genus Bifidobacterium.
29300153	0	25	theme	Bifidobacterium	0:14	arg1	sp					26:27	Bifidobacterium catulorum sp	0:27	Bifidobacterium catulorum sp.	0:28	Bifidobacterium catulorum sp.
29300153	5	26	theme	16S	911:913	arg1	rRNA					915:918	partial 16S rRNA	903:918	partial 16S rRNA	903:918	Another strain, designated MRM 8.19T, has been classified as member of the genus Bifidobacterium on the basis of positive results for fructose-6-phosphate phosphoketolase activity and analysis of partial 16S rRNA, hsp60, clpC, dnaJ, dnaG and rpoB gene sequences.
29300153	9	27	theme	strain	1456:1461	arg1	31794T					1489:1494	type strain MRM 8.19T=DSM 103154T=JCM 31794T	1451:1494	type strain MRM 8.19T=DSM 103154T=JCM 31794T	1451:1494	nov. (type strain MRM 8.19T=DSM 103154T=JCM 31794T) is proposed.
29300153	9	27	theme	strain	1456:1461	arg1	nov					1445:1447	nov	1445:1447	nov	1445:1447	nov. (type strain MRM 8.19T=DSM 103154T=JCM 31794T) is proposed.
29300153	8	28	theme	novel	1351:1355	arg1	taxon					1357:1361	a novel taxon	1349:1361	a novel taxon within the genus Bifidobacterium for which the name Bifidobacterium catulorum sp	1349:1442	Based on the phylogenetic, genotypic and phenotypic data reported, strain MRM 8.19T represents a novel taxon within the genus Bifidobacterium for which the name Bifidobacterium catulorum sp.
29300153	9	29	theme	MRM	1463:1465	arg1	31794T					1489:1494	type strain MRM 8.19T=DSM 103154T=JCM 31794T	1451:1494	type strain MRM 8.19T=DSM 103154T=JCM 31794T	1451:1494	nov. (type strain MRM 8.19T=DSM 103154T=JCM 31794T) is proposed.
29300153	9	29	theme	MRM	1463:1465	arg1	nov					1445:1447	nov	1445:1447	nov	1445:1447	nov. (type strain MRM 8.19T=DSM 103154T=JCM 31794T) is proposed.
29300153	7	30	theme	63.7 mol	1190:1197	arg1	composition					1174:1184	The DNA G+C composition	1162:1184	The DNA G+C composition	1162:1184	The DNA G+C composition was 63.7 mol% and the peptidoglycan structure was l-Orn(Lys)-l-Ser.
29300153	7	30	theme	63.7 mol	1190:1197	arg1	%					1198:1198	63.7 mol%	1190:1198	63.7 mol%	1190:1198	The DNA G+C composition was 63.7 mol% and the peptidoglycan structure was l-Orn(Lys)-l-Ser.
29300153	1	31	theme	baby	73:76	arg1	marmoset					85:92	the baby common marmoset	69:92	the baby common marmoset (Callithrix jacchus)	69:113	nov., a novel taxon from the faeces of the baby common marmoset (Callithrix jacchus).
29300153	2	32	theme	genus	407:411	arg1	Bifidobacterium					413:427	the genus Bifidobacterium	403:427	the genus Bifidobacterium	403:427	In our previous study based on hsp60 PCR-restriction fragment length polymorphism and 16S rRNA gene sequencing, we stated that the bifidobacterial strains isolated from the individual faecal samples of five baby common marmosets constituted different phylogenetically isolated groups of the genus Bifidobacterium.
29300153	2	33	theme	Bifidobacterium	413:427	arg1	Bifidobacterium					413:427	the genus Bifidobacterium	403:427	the genus Bifidobacterium	403:427	In our previous study based on hsp60 PCR-restriction fragment length polymorphism and 16S rRNA gene sequencing, we stated that the bifidobacterial strains isolated from the individual faecal samples of five baby common marmosets constituted different phylogenetically isolated groups of the genus Bifidobacterium.
29300153	2	33	theme	Bifidobacterium	413:427	arg1	groups					393:398	different phylogenetically isolated groups	357:398	different phylogenetically isolated groups of the genus Bifidobacterium	357:427	In our previous study based on hsp60 PCR-restriction fragment length polymorphism and 16S rRNA gene sequencing, we stated that the bifidobacterial strains isolated from the individual faecal samples of five baby common marmosets constituted different phylogenetically isolated groups of the genus Bifidobacterium.
29300153	5	34	theme	sequences	959:967	arg1	analysis					891:898	analysis	891:898	analysis of partial 16S rRNA, hsp60, clpC, dnaJ, dnaG and rpoB gene sequences	891:967	Another strain, designated MRM 8.19T, has been classified as member of the genus Bifidobacterium on the basis of positive results for fructose-6-phosphate phosphoketolase activity and analysis of partial 16S rRNA, hsp60, clpC, dnaJ, dnaG and rpoB gene sequences.
29300153	5	34	theme	sequences	959:967	arg1	activity					878:885	fructose-6-phosphate phosphoketolase activity	841:885	fructose-6-phosphate phosphoketolase activity	841:885	Another strain, designated MRM 8.19T, has been classified as member of the genus Bifidobacterium on the basis of positive results for fructose-6-phosphate phosphoketolase activity and analysis of partial 16S rRNA, hsp60, clpC, dnaJ, dnaG and rpoB gene sequences.
29300153	1	35	theme	common	78:83	arg1	marmoset					85:92	the baby common marmoset	69:92	the baby common marmoset (Callithrix jacchus)	69:113	nov., a novel taxon from the faeces of the baby common marmoset (Callithrix jacchus).
29300153	5	36	theme	partial	903:909	arg1	rRNA					915:918	partial 16S rRNA	903:918	partial 16S rRNA	903:918	Another strain, designated MRM 8.19T, has been classified as member of the genus Bifidobacterium on the basis of positive results for fructose-6-phosphate phosphoketolase activity and analysis of partial 16S rRNA, hsp60, clpC, dnaJ, dnaG and rpoB gene sequences.
29300153	2	37	attach	isolated	271:278	arg1	samples					307:313	the individual faecal samples	285:313	the individual faecal samples of five baby common marmosets	285:343	In our previous study based on hsp60 PCR-restriction fragment length polymorphism and 16S rRNA gene sequencing, we stated that the bifidobacterial strains isolated from the individual faecal samples of five baby common marmosets constituted different phylogenetically isolated groups of the genus Bifidobacterium.
29300153	2	37	attach	isolated	271:278	arg2	strains					263:269	the bifidobacterial strains	243:269	the bifidobacterial strains isolated from the individual faecal samples of five baby common marmosets	243:343	In our previous study based on hsp60 PCR-restriction fragment length polymorphism and 16S rRNA gene sequencing, we stated that the bifidobacterial strains isolated from the individual faecal samples of five baby common marmosets constituted different phylogenetically isolated groups of the genus Bifidobacterium.
29300153	5	38	theme	fructose-6-phosphate	841:860	arg1	activity					878:885	fructose-6-phosphate phosphoketolase activity	841:885	fructose-6-phosphate phosphoketolase activity	841:885	Another strain, designated MRM 8.19T, has been classified as member of the genus Bifidobacterium on the basis of positive results for fructose-6-phosphate phosphoketolase activity and analysis of partial 16S rRNA, hsp60, clpC, dnaJ, dnaG and rpoB gene sequences.
29300153	2	39	theme	rRNA	206:209	arg1	sequencing					216:225	16S rRNA gene sequencing	202:225	16S rRNA gene sequencing	202:225	In our previous study based on hsp60 PCR-restriction fragment length polymorphism and 16S rRNA gene sequencing, we stated that the bifidobacterial strains isolated from the individual faecal samples of five baby common marmosets constituted different phylogenetically isolated groups of the genus Bifidobacterium.
29300153	4	40	theme	Bifidobacterium	653:667	arg1	hapali					669:674	Bifidobacterium hapali	653:674	Bifidobacterium hapali	653:674	Out of them, Bifidobacterium aesculapii, Bifidobacterium myosotis, Bifidobacterium tissieri and Bifidobacterium hapali, have been described recently.
29300153	5	41	theme	genus	782:786	arg1	Bifidobacterium					788:802	the genus Bifidobacterium	778:802	the genus Bifidobacterium	778:802	Another strain, designated MRM 8.19T, has been classified as member of the genus Bifidobacterium on the basis of positive results for fructose-6-phosphate phosphoketolase activity and analysis of partial 16S rRNA, hsp60, clpC, dnaJ, dnaG and rpoB gene sequences.
29300153	5	42	theme	MRM	734:736	arg1	8.19T					738:742	MRM 8.19T	734:742	MRM 8.19T	734:742	Another strain, designated MRM 8.19T, has been classified as member of the genus Bifidobacterium on the basis of positive results for fructose-6-phosphate phosphoketolase activity and analysis of partial 16S rRNA, hsp60, clpC, dnaJ, dnaG and rpoB gene sequences.
29300153	8	43	theme	genus	1374:1378	arg1	Bifidobacterium					1380:1394	the genus Bifidobacterium	1370:1394	the genus Bifidobacterium for which the name Bifidobacterium catulorum sp	1370:1442	Based on the phylogenetic, genotypic and phenotypic data reported, strain MRM 8.19T represents a novel taxon within the genus Bifidobacterium for which the name Bifidobacterium catulorum sp.
29300153	2	44	theme	16S	202:204	arg1	sequencing					216:225	16S rRNA gene sequencing	202:225	16S rRNA gene sequencing	202:225	In our previous study based on hsp60 PCR-restriction fragment length polymorphism and 16S rRNA gene sequencing, we stated that the bifidobacterial strains isolated from the individual faecal samples of five baby common marmosets constituted different phylogenetically isolated groups of the genus Bifidobacterium.
29300153	5	45	theme	phosphoketolase	862:876	arg1	activity					878:885	fructose-6-phosphate phosphoketolase activity	841:885	fructose-6-phosphate phosphoketolase activity	841:885	Another strain, designated MRM 8.19T, has been classified as member of the genus Bifidobacterium on the basis of positive results for fructose-6-phosphate phosphoketolase activity and analysis of partial 16S rRNA, hsp60, clpC, dnaJ, dnaG and rpoB gene sequences.
29300153	5	46	theme	Bifidobacterium	788:802	arg1	member					768:773	member	768:773	member of the genus Bifidobacterium	768:802	Another strain, designated MRM 8.19T, has been classified as member of the genus Bifidobacterium on the basis of positive results for fructose-6-phosphate phosphoketolase activity and analysis of partial 16S rRNA, hsp60, clpC, dnaJ, dnaG and rpoB gene sequences.
29300153	5	46	theme	Bifidobacterium	788:802	arg1	strain					715:720	Another strain	707:720	Another strain	707:720	Another strain, designated MRM 8.19T, has been classified as member of the genus Bifidobacterium on the basis of positive results for fructose-6-phosphate phosphoketolase activity and analysis of partial 16S rRNA, hsp60, clpC, dnaJ, dnaG and rpoB gene sequences.
29300153	5	47	theme	positive	820:827	arg1	results					829:835	positive results	820:835	positive results for fructose-6-phosphate phosphoketolase activity and analysis of partial 16S rRNA, hsp60, clpC, dnaJ, dnaG and rpoB gene sequences	820:967	Another strain, designated MRM 8.19T, has been classified as member of the genus Bifidobacterium on the basis of positive results for fructose-6-phosphate phosphoketolase activity and analysis of partial 16S rRNA, hsp60, clpC, dnaJ, dnaG and rpoB gene sequences.
29300153	2	48	theme	previous	123:130	arg1	study					132:136	our previous study	119:136	our previous study based on hsp60 PCR-restriction fragment length polymorphism and 16S rRNA gene sequencing	119:225	In our previous study based on hsp60 PCR-restriction fragment length polymorphism and 16S rRNA gene sequencing, we stated that the bifidobacterial strains isolated from the individual faecal samples of five baby common marmosets constituted different phylogenetically isolated groups of the genus Bifidobacterium.
29300153	8	49	theme	MRM	1328:1330	arg1	8.19T					1332:1336	strain MRM 8.19T	1321:1336	strain MRM 8.19T	1321:1336	Based on the phylogenetic, genotypic and phenotypic data reported, strain MRM 8.19T represents a novel taxon within the genus Bifidobacterium for which the name Bifidobacterium catulorum sp.
29300153	6	50	theme	DSM	1074:1076	arg1	100201T					1078:1084	B. tissieri DSM 100201T	1062:1084	B. tissieri DSM 100201T (95.8 %)	1062:1093	Analysis of 16S rRNA and hsp60 gene sequences revealed that strain MRM 8.19T was related to B. tissieri DSM 100201T (95.8 %) and to Bifidobacterium bifidum ATCC 29521T (93.7 %), respectively.
29300153	6	50	theme	DSM	1074:1076	arg1	%					1092:1092	95.8 %	1087:1092	95.8 %	1087:1092	Analysis of 16S rRNA and hsp60 gene sequences revealed that strain MRM 8.19T was related to B. tissieri DSM 100201T (95.8 %) and to Bifidobacterium bifidum ATCC 29521T (93.7 %), respectively.
29300153	1	51	theme	marmoset	85:92	arg1	faeces					59:64	the faeces	55:64	the faeces of the baby common marmoset (Callithrix jacchus)	55:113	nov., a novel taxon from the faeces of the baby common marmoset (Callithrix jacchus).
29300153	5	52	theme	dnaG	940:943	arg1	analysis					891:898	analysis	891:898	analysis of partial 16S rRNA, hsp60, clpC, dnaJ, dnaG and rpoB gene sequences	891:967	Another strain, designated MRM 8.19T, has been classified as member of the genus Bifidobacterium on the basis of positive results for fructose-6-phosphate phosphoketolase activity and analysis of partial 16S rRNA, hsp60, clpC, dnaJ, dnaG and rpoB gene sequences.
29300153	5	52	theme	dnaG	940:943	arg1	activity					878:885	fructose-6-phosphate phosphoketolase activity	841:885	fructose-6-phosphate phosphoketolase activity	841:885	Another strain, designated MRM 8.19T, has been classified as member of the genus Bifidobacterium on the basis of positive results for fructose-6-phosphate phosphoketolase activity and analysis of partial 16S rRNA, hsp60, clpC, dnaJ, dnaG and rpoB gene sequences.
29300153	2	53	theme	length	178:183	arg1	polymorphism					185:196	hsp60 PCR-restriction fragment length polymorphism	147:196	hsp60 PCR-restriction fragment length polymorphism	147:196	In our previous study based on hsp60 PCR-restriction fragment length polymorphism and 16S rRNA gene sequencing, we stated that the bifidobacterial strains isolated from the individual faecal samples of five baby common marmosets constituted different phylogenetically isolated groups of the genus Bifidobacterium.
29300153	8	54	theme	strain	1321:1326	arg1	8.19T					1332:1336	strain MRM 8.19T	1321:1336	strain MRM 8.19T	1321:1336	Based on the phylogenetic, genotypic and phenotypic data reported, strain MRM 8.19T represents a novel taxon within the genus Bifidobacterium for which the name Bifidobacterium catulorum sp.
29300153	7	55	theme	peptidoglycan	1208:1220	arg1	-l-Ser					1246:1251	-l-Ser	1246:1251	-l-Ser	1246:1251	The DNA G+C composition was 63.7 mol% and the peptidoglycan structure was l-Orn(Lys)-l-Ser.
29300153	7	55	theme	peptidoglycan	1208:1220	arg1	structure					1222:1230	the peptidoglycan structure	1204:1230	the peptidoglycan structure	1204:1230	The DNA G+C composition was 63.7 mol% and the peptidoglycan structure was l-Orn(Lys)-l-Ser.
29300153	3	56	theme	isolated	473:480	arg1	groups					482:487	these isolated groups	467:487	these isolated groups	467:487	In that study, we also proposed that these isolated groups potentially represented novel species of the genus Bifidobacterium.
29300153	1	57	theme	novel	38:42	arg1	taxon					44:48	a novel taxon	36:48	a novel taxon from the faeces of the baby common marmoset (Callithrix jacchus)	36:113	nov., a novel taxon from the faeces of the baby common marmoset (Callithrix jacchus).
29300153	1	57	theme	novel	38:42	arg1	nov.					30:33	nov.	30:33	nov.	30:33	nov., a novel taxon from the faeces of the baby common marmoset (Callithrix jacchus).
29300153	2	58	theme	fragment	169:176	arg1	polymorphism					185:196	hsp60 PCR-restriction fragment length polymorphism	147:196	hsp60 PCR-restriction fragment length polymorphism	147:196	In our previous study based on hsp60 PCR-restriction fragment length polymorphism and 16S rRNA gene sequencing, we stated that the bifidobacterial strains isolated from the individual faecal samples of five baby common marmosets constituted different phylogenetically isolated groups of the genus Bifidobacterium.
29300153	8	59	theme	genotypic	1281:1289	arg1	data					1306:1309	the phylogenetic, genotypic and phenotypic data	1263:1309	data	1306:1309	Based on the phylogenetic, genotypic and phenotypic data reported, strain MRM 8.19T represents a novel taxon within the genus Bifidobacterium for which the name Bifidobacterium catulorum sp.
29300153	6	60	theme	gene	1001:1004	arg1	sequences					1006:1014	gene sequences	1001:1014	gene sequences	1001:1014	Analysis of 16S rRNA and hsp60 gene sequences revealed that strain MRM 8.19T was related to B. tissieri DSM 100201T (95.8 %) and to Bifidobacterium bifidum ATCC 29521T (93.7 %), respectively.
29300153	2	61	theme	faecal	300:305	arg1	samples					307:313	the individual faecal samples	285:313	the individual faecal samples of five baby common marmosets	285:343	In our previous study based on hsp60 PCR-restriction fragment length polymorphism and 16S rRNA gene sequencing, we stated that the bifidobacterial strains isolated from the individual faecal samples of five baby common marmosets constituted different phylogenetically isolated groups of the genus Bifidobacterium.
29300153	3	62	theme	Bifidobacterium	540:554	arg1	species					519:525	novel species	513:525	novel species of the genus Bifidobacterium	513:554	In that study, we also proposed that these isolated groups potentially represented novel species of the genus Bifidobacterium.
29300153	8	63	theme	phylogenetic	1267:1278	arg1	data					1306:1309	the phylogenetic, genotypic and phenotypic data	1263:1309	data	1306:1309	Based on the phylogenetic, genotypic and phenotypic data reported, strain MRM 8.19T represents a novel taxon within the genus Bifidobacterium for which the name Bifidobacterium catulorum sp.
29300153	2	64	theme	PCR-restriction	153:167	arg1	polymorphism					185:196	hsp60 PCR-restriction fragment length polymorphism	147:196	hsp60 PCR-restriction fragment length polymorphism	147:196	In our previous study based on hsp60 PCR-restriction fragment length polymorphism and 16S rRNA gene sequencing, we stated that the bifidobacterial strains isolated from the individual faecal samples of five baby common marmosets constituted different phylogenetically isolated groups of the genus Bifidobacterium.
29300153	5	65	theme	rpoB	949:952	arg1	sequences					959:967	rpoB gene sequences	949:967	rpoB gene sequences	949:967	Another strain, designated MRM 8.19T, has been classified as member of the genus Bifidobacterium on the basis of positive results for fructose-6-phosphate phosphoketolase activity and analysis of partial 16S rRNA, hsp60, clpC, dnaJ, dnaG and rpoB gene sequences.
29300153	2	66	theme	individual	289:298	arg1	samples					307:313	the individual faecal samples	285:313	the individual faecal samples of five baby common marmosets	285:343	In our previous study based on hsp60 PCR-restriction fragment length polymorphism and 16S rRNA gene sequencing, we stated that the bifidobacterial strains isolated from the individual faecal samples of five baby common marmosets constituted different phylogenetically isolated groups of the genus Bifidobacterium.
29300153	5	67	theme	clpC	928:931	arg1	analysis					891:898	analysis	891:898	analysis of partial 16S rRNA, hsp60, clpC, dnaJ, dnaG and rpoB gene sequences	891:967	Another strain, designated MRM 8.19T, has been classified as member of the genus Bifidobacterium on the basis of positive results for fructose-6-phosphate phosphoketolase activity and analysis of partial 16S rRNA, hsp60, clpC, dnaJ, dnaG and rpoB gene sequences.
29300153	5	67	theme	clpC	928:931	arg1	activity					878:885	fructose-6-phosphate phosphoketolase activity	841:885	fructose-6-phosphate phosphoketolase activity	841:885	Another strain, designated MRM 8.19T, has been classified as member of the genus Bifidobacterium on the basis of positive results for fructose-6-phosphate phosphoketolase activity and analysis of partial 16S rRNA, hsp60, clpC, dnaJ, dnaG and rpoB gene sequences.
29300153	7	68	theme	DNA	1166:1168	arg1	composition					1174:1184	The DNA G+C composition	1162:1184	The DNA G+C composition	1162:1184	The DNA G+C composition was 63.7 mol% and the peptidoglycan structure was l-Orn(Lys)-l-Ser.
29300153	7	68	theme	DNA	1166:1168	arg1	%					1198:1198	63.7 mol%	1190:1198	63.7 mol%	1190:1198	The DNA G+C composition was 63.7 mol% and the peptidoglycan structure was l-Orn(Lys)-l-Ser.
29300153	2	69	theme	hsp60	147:151	arg1	polymorphism					185:196	hsp60 PCR-restriction fragment length polymorphism	147:196	hsp60 PCR-restriction fragment length polymorphism	147:196	In our previous study based on hsp60 PCR-restriction fragment length polymorphism and 16S rRNA gene sequencing, we stated that the bifidobacterial strains isolated from the individual faecal samples of five baby common marmosets constituted different phylogenetically isolated groups of the genus Bifidobacterium.
29300153	5	70	dep	results	829:835	arg1	basis					811:815	basis	811:815	basis	811:815	Another strain, designated MRM 8.19T, has been classified as member of the genus Bifidobacterium on the basis of positive results for fructose-6-phosphate phosphoketolase activity and analysis of partial 16S rRNA, hsp60, clpC, dnaJ, dnaG and rpoB gene sequences.
29300153	5	70	dep	results	829:835	arg1	the					807:809	the	807:809	the	807:809	Another strain, designated MRM 8.19T, has been classified as member of the genus Bifidobacterium on the basis of positive results for fructose-6-phosphate phosphoketolase activity and analysis of partial 16S rRNA, hsp60, clpC, dnaJ, dnaG and rpoB gene sequences.
29300153	6	71	theme	MRM	1037:1039	arg1	8.19T					1041:1045	strain MRM 8.19T	1030:1045	strain MRM 8.19T	1030:1045	Analysis of 16S rRNA and hsp60 gene sequences revealed that strain MRM 8.19T was related to B. tissieri DSM 100201T (95.8 %) and to Bifidobacterium bifidum ATCC 29521T (93.7 %), respectively.
29300153	2	72	theme	marmosets	335:343	arg1	samples					307:313	the individual faecal samples	285:313	the individual faecal samples of five baby common marmosets	285:343	In our previous study based on hsp60 PCR-restriction fragment length polymorphism and 16S rRNA gene sequencing, we stated that the bifidobacterial strains isolated from the individual faecal samples of five baby common marmosets constituted different phylogenetically isolated groups of the genus Bifidobacterium.
29300153	6	73	dep	B.	1062:1063	arg1	tissieri					1065:1072	tissieri	1065:1072	tissieri	1065:1072	Analysis of 16S rRNA and hsp60 gene sequences revealed that strain MRM 8.19T was related to B. tissieri DSM 100201T (95.8 %) and to Bifidobacterium bifidum ATCC 29521T (93.7 %), respectively.
29300153	6	74	theme	hsp60	995:999	arg1	Analysis					970:977	Analysis	970:977	Analysis of 16S rRNA and hsp60 gene sequences	970:1014	Analysis of 16S rRNA and hsp60 gene sequences revealed that strain MRM 8.19T was related to B. tissieri DSM 100201T (95.8 %) and to Bifidobacterium bifidum ATCC 29521T (93.7 %), respectively.
29300153	7	75	theme	G+C	1170:1172	arg1	composition					1174:1184	The DNA G+C composition	1162:1184	The DNA G+C composition	1162:1184	The DNA G+C composition was 63.7 mol% and the peptidoglycan structure was l-Orn(Lys)-l-Ser.
29300153	7	75	theme	G+C	1170:1172	arg1	%					1198:1198	63.7 mol%	1190:1198	63.7 mol%	1190:1198	The DNA G+C composition was 63.7 mol% and the peptidoglycan structure was l-Orn(Lys)-l-Ser.
29300153	8	76	theme	Bifidobacterium	1415:1429	arg1	sp					1441:1442	the name Bifidobacterium catulorum sp	1406:1442	the name Bifidobacterium catulorum sp	1406:1442	Based on the phylogenetic, genotypic and phenotypic data reported, strain MRM 8.19T represents a novel taxon within the genus Bifidobacterium for which the name Bifidobacterium catulorum sp.
29300153	2	77	theme	gene	211:214	arg1	sequencing					216:225	16S rRNA gene sequencing	202:225	16S rRNA gene sequencing	202:225	In our previous study based on hsp60 PCR-restriction fragment length polymorphism and 16S rRNA gene sequencing, we stated that the bifidobacterial strains isolated from the individual faecal samples of five baby common marmosets constituted different phylogenetically isolated groups of the genus Bifidobacterium.
29300153	9	78	theme	type	1451:1454	arg1	31794T					1489:1494	type strain MRM 8.19T=DSM 103154T=JCM 31794T	1451:1494	type strain MRM 8.19T=DSM 103154T=JCM 31794T	1451:1494	nov. (type strain MRM 8.19T=DSM 103154T=JCM 31794T) is proposed.
29300153	9	78	theme	type	1451:1454	arg1	nov					1445:1447	nov	1445:1447	nov	1445:1447	nov. (type strain MRM 8.19T=DSM 103154T=JCM 31794T) is proposed.
29300153	2	79	theme	common	328:333	arg1	marmosets					335:343	five baby common marmosets	318:343	five baby common marmosets	318:343	In our previous study based on hsp60 PCR-restriction fragment length polymorphism and 16S rRNA gene sequencing, we stated that the bifidobacterial strains isolated from the individual faecal samples of five baby common marmosets constituted different phylogenetically isolated groups of the genus Bifidobacterium.
30889640	0	0	theme	-chitosan/alumina	74:90	arg1	nanowires					92:100	poly (3-hydroxybutyrate)-chitosan/alumina nanowires	50:100	poly (3-hydroxybutyrate)-chitosan/alumina nanowires	50:100	Potential of an electrospun composite scaffold of poly (3-hydroxybutyrate)-chitosan/alumina nanowires in bone tissue engineering applications.
30889640	1	1	theme	material	157:164	arg1	types					166:170	material types	157:170	material types for tissue engineering scaffolds and the design of methods	157:229	The choice of material types for tissue engineering scaffolds and the design of methods are contributive in yielding the proper result.
30889640	10	2	theme	cells	1031:1035	arg1	Proliferation					994:1006	Proliferation	994:1006	Proliferation	994:1006	Proliferation and viability of MG-63 cells and alkaline phosphatase secretion are significantly higher on scaffolds containing Alumina than that of the PHB or PHB-CTS.
30889640	10	2	theme	cells	1031:1035	arg1	secretion					1062:1070	alkaline phosphatase secretion	1041:1070	alkaline phosphatase secretion	1041:1070	Proliferation and viability of MG-63 cells and alkaline phosphatase secretion are significantly higher on scaffolds containing Alumina than that of the PHB or PHB-CTS.
30889640	10	2	theme	cells	1031:1035	arg1	viability					1012:1020	viability	1012:1020	viability	1012:1020	Proliferation and viability of MG-63 cells and alkaline phosphatase secretion are significantly higher on scaffolds containing Alumina than that of the PHB or PHB-CTS.
30889640	3	3	theme	Alumina	303:309	arg1	nanowires					311:319	Alumina nanowires	303:319	Alumina nanowires	303:319	Alumina nanowires are added to (Polyhydroxybutyrate-Chitosan) PHB-CTS alloy solution, and the scaffolds are prepared by electrospinning method.
30889640	11	4	theme	cell	1218:1221	arg1	behavior					1223:1230	cell behavior	1218:1230	cell behavior	1218:1230	The appropriate properties of Alumina which affected in cell behavior, hydrophilicity enhancement, bioactivity and mechanical properties make it contribution agent in bone tissue engineering.
30889640	5	5	theme	surface	573:579	arg1	roughness					581:589	The surface roughness	569:589	The surface roughness of the fibers	569:603	The surface roughness of the fibers is confirmed by FESEM and AFM.
30889640	1	6	theme	types	166:170	arg1	contributive					235:246	contributive	235:246	contributive	235:246	The choice of material types for tissue engineering scaffolds and the design of methods are contributive in yielding the proper result.
30889640	1	6	theme	types	166:170	arg1	choice					147:152	The choice	143:152	The choice of material types for tissue engineering scaffolds and the design of methods	143:229	The choice of material types for tissue engineering scaffolds and the design of methods are contributive in yielding the proper result.
30889640	2	7	from	wt	299:300	arg1	study					287:291	this study	282:291	this study	282:291	In this study, 1-5% wt.
30889640	9	8	theme	Alumina	875:881	arg1	scaffolds					894:902	Alumina containing scaffolds	875:902	Alumina containing scaffolds	875:902	Formation of calcium phosphate sediments only on the surface of Alumina containing scaffolds after 7 and 28 days of immersion in SBF is observed by SEM, and verified by XRD analysis.
30889640	0	9	from	Potential	0:8	arg1	applications					129:140	bone tissue engineering applications	105:140	bone tissue engineering applications	105:140	Potential of an electrospun composite scaffold of poly (3-hydroxybutyrate)-chitosan/alumina nanowires in bone tissue engineering applications.
30889640	10	10	contain	containing	1110:1119	arg1	scaffolds					1100:1108	scaffolds	1100:1108	scaffolds containing Alumina	1100:1127	Proliferation and viability of MG-63 cells and alkaline phosphatase secretion are significantly higher on scaffolds containing Alumina than that of the PHB or PHB-CTS.
30889640	10	10	contain	containing	1110:1119	arg2	Alumina					1121:1127	Alumina	1121:1127	Alumina	1121:1127	Proliferation and viability of MG-63 cells and alkaline phosphatase secretion are significantly higher on scaffolds containing Alumina than that of the PHB or PHB-CTS.
30889640	9	11	theme	XRD	980:982	arg1	analysis					984:991	XRD analysis	980:991	XRD analysis	980:991	Formation of calcium phosphate sediments only on the surface of Alumina containing scaffolds after 7 and 28 days of immersion in SBF is observed by SEM, and verified by XRD analysis.
30889640	1	12	theme	proper	264:269	arg1	result					271:276	the proper result	260:276	the proper result	260:276	The choice of material types for tissue engineering scaffolds and the design of methods are contributive in yielding the proper result.
30889640	0	13	theme	nanowires	92:100	arg1	scaffold					38:45	an electrospun composite scaffold	13:45	an electrospun composite scaffold of poly (3-hydroxybutyrate)-chitosan/alumina nanowires	13:100	Potential of an electrospun composite scaffold of poly (3-hydroxybutyrate)-chitosan/alumina nanowires in bone tissue engineering applications.
30889640	9	14	from	28 days	916:922	arg1	SBF					940:942	SBF	940:942	SBF	940:942	Formation of calcium phosphate sediments only on the surface of Alumina containing scaffolds after 7 and 28 days of immersion in SBF is observed by SEM, and verified by XRD analysis.
30889640	7	15	theme	wt	798:799	arg1	presence					783:790	presence	783:790	presence of 3% wt	783:799	The tensile strength of the PHB-CTS scaffold increase up to >10 fold in presence of 3% wt.
30889640	0	16	theme	tissue	110:115	arg1	applications					129:140	bone tissue engineering applications	105:140	bone tissue engineering applications	105:140	Potential of an electrospun composite scaffold of poly (3-hydroxybutyrate)-chitosan/alumina nanowires in bone tissue engineering applications.
30889640	10	17	theme	MG-63	1025:1029	arg1	cells					1031:1035	MG-63 cells	1025:1035	MG-63 cells	1025:1035	Proliferation and viability of MG-63 cells and alkaline phosphatase secretion are significantly higher on scaffolds containing Alumina than that of the PHB or PHB-CTS.
30889640	5	18	theme	fibers	598:603	arg1	roughness					581:589	The surface roughness	569:589	The surface roughness of the fibers	569:603	The surface roughness of the fibers is confirmed by FESEM and AFM.
30889640	0	19	theme	bone	105:108	arg1	applications					129:140	bone tissue engineering applications	105:140	bone tissue engineering applications	105:140	Potential of an electrospun composite scaffold of poly (3-hydroxybutyrate)-chitosan/alumina nanowires in bone tissue engineering applications.
30889640	9	20	theme	immersion	927:935	arg1	28 days					916:922	28 days	916:922	28 days of immersion in SBF	916:942	Formation of calcium phosphate sediments only on the surface of Alumina containing scaffolds after 7 and 28 days of immersion in SBF is observed by SEM, and verified by XRD analysis.
30889640	9	21	theme	scaffolds	894:902	arg1	surface					864:870	the surface	860:870	the surface of Alumina containing scaffolds	860:902	Formation of calcium phosphate sediments only on the surface of Alumina containing scaffolds after 7 and 28 days of immersion in SBF is observed by SEM, and verified by XRD analysis.
30889640	7	22	theme	3	795:795	arg1	%					796:796	%	796:796	%	796:796	The tensile strength of the PHB-CTS scaffold increase up to >10 fold in presence of 3% wt.
30889640	1	23	theme	tissue	176:181	arg1	scaffolds					195:203	tissue engineering scaffolds	176:203	tissue engineering scaffolds	176:203	The choice of material types for tissue engineering scaffolds and the design of methods are contributive in yielding the proper result.
30889640	3	24	theme	alloy	373:377	arg1	solution					379:386	PHB-CTS alloy solution	365:386	(Polyhydroxybutyrate-Chitosan) PHB-CTS alloy solution	334:386	Alumina nanowires are added to (Polyhydroxybutyrate-Chitosan) PHB-CTS alloy solution, and the scaffolds are prepared by electrospinning method.
30889640	3	24	theme	alloy	373:377	arg1	Polyhydroxybutyrate-Chitosan					335:362	Polyhydroxybutyrate-Chitosan	335:362	Polyhydroxybutyrate-Chitosan	335:362	Alumina nanowires are added to (Polyhydroxybutyrate-Chitosan) PHB-CTS alloy solution, and the scaffolds are prepared by electrospinning method.
30889640	4	25	theme	porosity	468:475	arg1	percentages					477:487	porosity percentages	468:487	porosity percentages	468:487	The fiber diameters, porosity percentages and uniform distribution of Alumina nanowires are assessed by SEM, EDS and TEM.
30889640	11	26	dep	it	1304:1305	arg1	agent					1320:1324	contribution agent	1307:1324	it contribution agent in bone tissue engineering	1304:1351	The appropriate properties of Alumina which affected in cell behavior, hydrophilicity enhancement, bioactivity and mechanical properties make it contribution agent in bone tissue engineering.
30889640	7	27	theme	%	796:796	arg1	wt					798:799	3% wt	795:799	3% wt	795:799	The tensile strength of the PHB-CTS scaffold increase up to >10 fold in presence of 3% wt.
30889640	1	28	theme	engineering	183:193	arg1	scaffolds					195:203	tissue engineering scaffolds	176:203	tissue engineering scaffolds	176:203	The choice of material types for tissue engineering scaffolds and the design of methods are contributive in yielding the proper result.
30889640	11	29	theme	mechanical	1277:1286	arg1	properties					1288:1297	mechanical properties	1277:1297	mechanical properties	1277:1297	The appropriate properties of Alumina which affected in cell behavior, hydrophilicity enhancement, bioactivity and mechanical properties make it contribution agent in bone tissue engineering.
30889640	11	30	theme	hydrophilicity	1233:1246	arg1	enhancement					1248:1258	hydrophilicity enhancement	1233:1258	hydrophilicity enhancement	1233:1258	The appropriate properties of Alumina which affected in cell behavior, hydrophilicity enhancement, bioactivity and mechanical properties make it contribution agent in bone tissue engineering.
30889640	6	31	theme	nanofibers	657:666	arg1	crystallinity					640:652	The crystallinity	636:652	The crystallinity of nanofibers	636:666	The crystallinity of nanofibers is calculated by DSC and verified by FTIR.
30889640	0	32	theme	electrospun	16:26	arg1	scaffold					38:45	an electrospun composite scaffold	13:45	an electrospun composite scaffold of poly (3-hydroxybutyrate)-chitosan/alumina nanowires	13:100	Potential of an electrospun composite scaffold of poly (3-hydroxybutyrate)-chitosan/alumina nanowires in bone tissue engineering applications.
30889640	10	33	theme	phosphatase	1050:1060	arg1	secretion					1062:1070	alkaline phosphatase secretion	1041:1070	alkaline phosphatase secretion	1041:1070	Proliferation and viability of MG-63 cells and alkaline phosphatase secretion are significantly higher on scaffolds containing Alumina than that of the PHB or PHB-CTS.
30889640	9	34	theme	phosphate	832:840	arg1	Formation					811:819	Formation	811:819	Formation of calcium phosphate	811:840	Formation of calcium phosphate sediments only on the surface of Alumina containing scaffolds after 7 and 28 days of immersion in SBF is observed by SEM, and verified by XRD analysis.
30889640	3	35	theme	PHB-CTS	365:371	arg1	solution					379:386	PHB-CTS alloy solution	365:386	(Polyhydroxybutyrate-Chitosan) PHB-CTS alloy solution	334:386	Alumina nanowires are added to (Polyhydroxybutyrate-Chitosan) PHB-CTS alloy solution, and the scaffolds are prepared by electrospinning method.
30889640	3	35	theme	PHB-CTS	365:371	arg1	Polyhydroxybutyrate-Chitosan					335:362	Polyhydroxybutyrate-Chitosan	335:362	Polyhydroxybutyrate-Chitosan	335:362	Alumina nanowires are added to (Polyhydroxybutyrate-Chitosan) PHB-CTS alloy solution, and the scaffolds are prepared by electrospinning method.
30889640	10	36	theme	alkaline	1041:1048	arg1	phosphatase					1050:1060	alkaline phosphatase	1041:1060	alkaline phosphatase secretion	1041:1070	Proliferation and viability of MG-63 cells and alkaline phosphatase secretion are significantly higher on scaffolds containing Alumina than that of the PHB or PHB-CTS.
30889640	0	37	theme	engineering	117:127	arg1	applications					129:140	bone tissue engineering applications	105:140	bone tissue engineering applications	105:140	Potential of an electrospun composite scaffold of poly (3-hydroxybutyrate)-chitosan/alumina nanowires in bone tissue engineering applications.
30889640	0	38	theme	scaffold	38:45	arg1	Potential					0:8	Potential	0:8	Potential of an electrospun composite scaffold of poly (3-hydroxybutyrate)-chitosan/alumina nanowires in bone tissue engineering applications.	0:141	Potential of an electrospun composite scaffold of poly (3-hydroxybutyrate)-chitosan/alumina nanowires in bone tissue engineering applications.
30889640	11	39	theme	Alumina	1192:1198	arg1	properties					1178:1187	The appropriate properties	1162:1187	The appropriate properties of Alumina which affected in cell behavior, hydrophilicity enhancement, bioactivity and mechanical properties	1162:1297	The appropriate properties of Alumina which affected in cell behavior, hydrophilicity enhancement, bioactivity and mechanical properties make it contribution agent in bone tissue engineering.
30889640	0	40	theme	composite	28:36	arg1	scaffold					38:45	an electrospun composite scaffold	13:45	an electrospun composite scaffold of poly (3-hydroxybutyrate)-chitosan/alumina nanowires	13:100	Potential of an electrospun composite scaffold of poly (3-hydroxybutyrate)-chitosan/alumina nanowires in bone tissue engineering applications.
30889640	4	41	theme	nanowires	525:533	arg1	diameters					457:465	The fiber diameters	447:465	The fiber diameters	447:465	The fiber diameters, porosity percentages and uniform distribution of Alumina nanowires are assessed by SEM, EDS and TEM.
30889640	4	41	theme	nanowires	525:533	arg1	percentages					477:487	porosity percentages	468:487	porosity percentages	468:487	The fiber diameters, porosity percentages and uniform distribution of Alumina nanowires are assessed by SEM, EDS and TEM.
30889640	4	41	theme	nanowires	525:533	arg1	distribution					501:512	uniform distribution	493:512	uniform distribution	493:512	The fiber diameters, porosity percentages and uniform distribution of Alumina nanowires are assessed by SEM, EDS and TEM.
30889640	11	42	theme	contribution	1307:1318	arg1	agent					1320:1324	contribution agent	1307:1324	it contribution agent in bone tissue engineering	1304:1351	The appropriate properties of Alumina which affected in cell behavior, hydrophilicity enhancement, bioactivity and mechanical properties make it contribution agent in bone tissue engineering.
30889640	4	43	theme	fiber	451:455	arg1	diameters					457:465	The fiber diameters	447:465	The fiber diameters	447:465	The fiber diameters, porosity percentages and uniform distribution of Alumina nanowires are assessed by SEM, EDS and TEM.
30889640	9	44	theme	containing	883:892	arg1	scaffolds					894:902	Alumina containing scaffolds	875:902	Alumina containing scaffolds	875:902	Formation of calcium phosphate sediments only on the surface of Alumina containing scaffolds after 7 and 28 days of immersion in SBF is observed by SEM, and verified by XRD analysis.
30889640	4	45	theme	Alumina	517:523	arg1	nanowires					525:533	Alumina nanowires	517:533	Alumina nanowires	517:533	The fiber diameters, porosity percentages and uniform distribution of Alumina nanowires are assessed by SEM, EDS and TEM.
30889640	9	46	theme	calcium	824:830	arg1	phosphate					832:840	calcium phosphate	824:840	calcium phosphate	824:840	Formation of calcium phosphate sediments only on the surface of Alumina containing scaffolds after 7 and 28 days of immersion in SBF is observed by SEM, and verified by XRD analysis.
30889640	7	47	theme	PHB-CTS	739:745	arg1	scaffold					747:754	the PHB-CTS scaffold	735:754	the PHB-CTS scaffold	735:754	The tensile strength of the PHB-CTS scaffold increase up to >10 fold in presence of 3% wt.
30889640	11	48	from	agent	1320:1324	arg1	engineering					1341:1351	bone tissue engineering	1329:1351	bone tissue engineering	1329:1351	The appropriate properties of Alumina which affected in cell behavior, hydrophilicity enhancement, bioactivity and mechanical properties make it contribution agent in bone tissue engineering.
30889640	10	49	dep	PHB	1146:1148	arg1	the					1142:1144	the	1142:1144	the	1142:1144	Proliferation and viability of MG-63 cells and alkaline phosphatase secretion are significantly higher on scaffolds containing Alumina than that of the PHB or PHB-CTS.
30889640	11	50	theme	appropriate	1166:1176	arg1	properties					1178:1187	The appropriate properties	1162:1187	The appropriate properties of Alumina which affected in cell behavior, hydrophilicity enhancement, bioactivity and mechanical properties	1162:1297	The appropriate properties of Alumina which affected in cell behavior, hydrophilicity enhancement, bioactivity and mechanical properties make it contribution agent in bone tissue engineering.
30889640	1	51	theme	methods	223:229	arg1	scaffolds					195:203	tissue engineering scaffolds	176:203	tissue engineering scaffolds	176:203	The choice of material types for tissue engineering scaffolds and the design of methods are contributive in yielding the proper result.
30889640	1	51	theme	methods	223:229	arg1	design					213:218	the design	209:218	the design of methods	209:229	The choice of material types for tissue engineering scaffolds and the design of methods are contributive in yielding the proper result.
30889640	2	52	from	study	287:291	arg1	wt					299:300	wt	299:300	wt	299:300	In this study, 1-5% wt.
30889640	11	53	theme	bone	1329:1332	arg1	engineering					1341:1351	bone tissue engineering	1329:1351	bone tissue engineering	1329:1351	The appropriate properties of Alumina which affected in cell behavior, hydrophilicity enhancement, bioactivity and mechanical properties make it contribution agent in bone tissue engineering.
30889640	11	54	theme	tissue	1334:1339	arg1	engineering					1341:1351	bone tissue engineering	1329:1351	bone tissue engineering	1329:1351	The appropriate properties of Alumina which affected in cell behavior, hydrophilicity enhancement, bioactivity and mechanical properties make it contribution agent in bone tissue engineering.
30889640	4	55	theme	uniform	493:499	arg1	distribution					501:512	uniform distribution	493:512	uniform distribution	493:512	The fiber diameters, porosity percentages and uniform distribution of Alumina nanowires are assessed by SEM, EDS and TEM.
30889640	7	56	theme	tensile	715:721	arg1	strength					723:730	The tensile strength	711:730	The tensile strength of the PHB-CTS scaffold	711:754	The tensile strength of the PHB-CTS scaffold increase up to >10 fold in presence of 3% wt.
30889640	7	57	theme	scaffold	747:754	arg1	strength					723:730	The tensile strength	711:730	The tensile strength of the PHB-CTS scaffold	711:754	The tensile strength of the PHB-CTS scaffold increase up to >10 fold in presence of 3% wt.
31238493	9	0	from	positions	1503:1511	arg1	residues					1491:1498	the three E-GL residues	1476:1498	the three E-GL residues at positions E-152, E-156, and E-158	1476:1535	From our data, we propose that the three E-GL residues at positions E-152, E-156, and E-158 greatly influence the accessibility of neutralizing antibody epitopes on ZIKV.
31238493	2	1	theme	defects	335:341	arg1	cause					279:283	the cause	275:283	the cause of congenital Zika disease and other neurological defects	275:341	ZIKV infection is recognized as the cause of congenital Zika disease and other neurological defects, with no specific prophylactic or therapeutic treatments.
31238493	2	1	theme	defects	335:341	arg1	infection					248:256	ZIKV infection	243:256	ZIKV infection	243:256	ZIKV infection is recognized as the cause of congenital Zika disease and other neurological defects, with no specific prophylactic or therapeutic treatments.
31238493	9	2	from	accessibility	1559:1571	arg1	ZIKV					1610:1613	ZIKV	1610:1613	ZIKV	1610:1613	From our data, we propose that the three E-GL residues at positions E-152, E-156, and E-158 greatly influence the accessibility of neutralizing antibody epitopes on ZIKV.
31238493	1	3	theme	health	227:232	arg1	concern					234:240	major public health concern	214:240	major public health concern	214:240	Zika virus (ZIKV) is an emerging mosquito-borne flavivirus which is of major public health concern.
31238493	8	4	theme	Antibody-mediated	1263:1279	arg1	neutralization					1286:1299	Antibody-mediated ZIKV neutralization	1263:1299	Antibody-mediated ZIKV neutralization	1263:1299	Antibody-mediated ZIKV neutralization was effective against viral strain MR766, whereas epidemic ZIKV strains were poorly sensitive to neutralization by anti-ZIKBeHMR-2 immune sera.
31238493	2	5	theme	neurological	322:333	arg1	defects					335:341	other neurological defects	316:341	other neurological defects	316:341	ZIKV infection is recognized as the cause of congenital Zika disease and other neurological defects, with no specific prophylactic or therapeutic treatments.
31238493	6	6	theme	Adult	983:987	arg1	mice					996:999	Adult BALB/c mice	983:999	Adult BALB/c mice inoculated intraperitoneally with ZIKBeHMR-2	983:1044	Adult BALB/c mice inoculated intraperitoneally with ZIKBeHMR-2 developed anti-ZIKV antibodies directed against viral proteins E and NS1 and a booster dose increased antibody titers.
31238493	6	7	dep	proteins	1100:1107	arg1	E					1109:1109	E	1109:1109	E	1109:1109	Adult BALB/c mice inoculated intraperitoneally with ZIKBeHMR-2 developed anti-ZIKV antibodies directed against viral proteins E and NS1 and a booster dose increased antibody titers.
31238493	6	7	dep	proteins	1100:1107	arg1	proteins					1100:1107	viral proteins E and NS1	1094:1117	viral proteins E and NS1	1094:1117	Adult BALB/c mice inoculated intraperitoneally with ZIKBeHMR-2 developed anti-ZIKV antibodies directed against viral proteins E and NS1 and a booster dose increased antibody titers.
31238493	6	7	dep	proteins	1100:1107	arg1	NS1					1115:1117	NS1	1115:1117	NS1	1115:1117	Adult BALB/c mice inoculated intraperitoneally with ZIKBeHMR-2 developed anti-ZIKV antibodies directed against viral proteins E and NS1 and a booster dose increased antibody titers.
31238493	8	8	theme	ZIKV	1360:1363	arg1	strains					1365:1371	epidemic ZIKV strains	1351:1371	epidemic ZIKV strains	1351:1371	Antibody-mediated ZIKV neutralization was effective against viral strain MR766, whereas epidemic ZIKV strains were poorly sensitive to neutralization by anti-ZIKBeHMR-2 immune sera.
31238493	4	9	theme	epidemic	798:805	arg1	BeH819015					814:822	the epidemic strain BeH819015	794:822	the epidemic strain BeH819015	794:822	In the present study, we evaluate the immunogenicity of chimeric viral clone ZIKBeHMR-2, in which the region encoding the structural proteins of the African strain MR766 backbone was replaced with its counterpart from the epidemic strain BeH819015.
31238493	3	10	theme	effective	510:518	arg1	vaccines					520:527	effective vaccines	510:527	effective vaccines that confer protection against ZIKV infection	510:573	As the humoral immune response is an essential component of protective immunity, there is an urgent need for effective vaccines that confer protection against ZIKV infection.
31238493	9	11	theme	epitopes	1598:1605	arg1	accessibility					1559:1571	the accessibility	1555:1571	the accessibility of neutralizing antibody epitopes on ZIKV	1555:1613	From our data, we propose that the three E-GL residues at positions E-152, E-156, and E-158 greatly influence the accessibility of neutralizing antibody epitopes on ZIKV.
31238493	5	12	theme	glycan	903:908	arg1	loop					910:913	the glycan loop	899:913	the glycan loop of the E protein (E-GL) making ZIKBeHMR-2 a non-glycosylated virus	899:980	Three amino-acid substitutions I152T, T156I, and H158Y were introduced in the glycan loop of the E protein (E-GL) making ZIKBeHMR-2 a non-glycosylated virus.
31238493	6	13	theme	booster	1125:1131	arg1	dose					1133:1136	a booster dose	1123:1136	a booster dose	1123:1136	Adult BALB/c mice inoculated intraperitoneally with ZIKBeHMR-2 developed anti-ZIKV antibodies directed against viral proteins E and NS1 and a booster dose increased antibody titers.
31238493	5	14	theme	protein	924:930	arg1	loop					910:913	the glycan loop	899:913	the glycan loop of the E protein (E-GL) making ZIKBeHMR-2 a non-glycosylated virus	899:980	Three amino-acid substitutions I152T, T156I, and H158Y were introduced in the glycan loop of the E protein (E-GL) making ZIKBeHMR-2 a non-glycosylated virus.
31238493	4	15	theme	African	725:731	arg1	backbone					746:753	the African strain MR766 backbone	721:753	the African strain MR766 backbone	721:753	In the present study, we evaluate the immunogenicity of chimeric viral clone ZIKBeHMR-2, in which the region encoding the structural proteins of the African strain MR766 backbone was replaced with its counterpart from the epidemic strain BeH819015.
31238493	5	16	dep	ZIKBeHMR-2	946:955	arg1	virus					976:980	a non-glycosylated virus	957:980	ZIKBeHMR-2 a non-glycosylated virus	946:980	Three amino-acid substitutions I152T, T156I, and H158Y were introduced in the glycan loop of the E protein (E-GL) making ZIKBeHMR-2 a non-glycosylated virus.
31238493	3	17	theme	protective	461:470	arg1	immunity					472:479	protective immunity	461:479	protective immunity	461:479	As the humoral immune response is an essential component of protective immunity, there is an urgent need for effective vaccines that confer protection against ZIKV infection.
31238493	3	18	theme	immunity	472:479	arg1	response					423:430	the humoral immune response	404:430	the humoral immune response	404:430	As the humoral immune response is an essential component of protective immunity, there is an urgent need for effective vaccines that confer protection against ZIKV infection.
31238493	3	18	theme	immunity	472:479	arg1	component					448:456	an essential component	435:456	an essential component of protective immunity	435:479	As the humoral immune response is an essential component of protective immunity, there is an urgent need for effective vaccines that confer protection against ZIKV infection.
31238493	6	19	theme	anti-ZIKV	1056:1064	arg1	antibodies					1066:1075	anti-ZIKV antibodies	1056:1075	anti-ZIKV antibodies directed against viral proteins E and NS1	1056:1117	Adult BALB/c mice inoculated intraperitoneally with ZIKBeHMR-2 developed anti-ZIKV antibodies directed against viral proteins E and NS1 and a booster dose increased antibody titers.
31238493	4	20	theme	backbone	746:753	arg1	proteins					709:716	the structural proteins	694:716	the structural proteins of the African strain MR766 backbone	694:753	In the present study, we evaluate the immunogenicity of chimeric viral clone ZIKBeHMR-2, in which the region encoding the structural proteins of the African strain MR766 backbone was replaced with its counterpart from the epidemic strain BeH819015.
31238493	4	21	theme	clone	647:651	arg1	ZIKBeHMR-2					653:662	chimeric viral clone ZIKBeHMR-2	632:662	chimeric viral clone ZIKBeHMR-2	632:662	In the present study, we evaluate the immunogenicity of chimeric viral clone ZIKBeHMR-2, in which the region encoding the structural proteins of the African strain MR766 backbone was replaced with its counterpart from the epidemic strain BeH819015.
31238493	3	22	theme	immune	416:421	arg1	response					423:430	the humoral immune response	404:430	the humoral immune response	404:430	As the humoral immune response is an essential component of protective immunity, there is an urgent need for effective vaccines that confer protection against ZIKV infection.
31238493	3	22	theme	immune	416:421	arg1	component					448:456	an essential component	435:456	an essential component of protective immunity	435:479	As the humoral immune response is an essential component of protective immunity, there is an urgent need for effective vaccines that confer protection against ZIKV infection.
31238493	0	23	theme	E-glycan	86:93	arg1	Loop					95:98	E-glycan Loop	86:98	E-glycan Loop	86:98	A Chimeric Zika Virus between Viral Strains MR766 and BeH819015 Highlights a Role for E-glycan Loop in Antibody-mediated Virus Neutralization.
31238493	4	24	from	BeH819015	814:822	arg1	counterpart					777:787	its counterpart	773:787	its counterpart from the epidemic strain BeH819015	773:822	In the present study, we evaluate the immunogenicity of chimeric viral clone ZIKBeHMR-2, in which the region encoding the structural proteins of the African strain MR766 backbone was replaced with its counterpart from the epidemic strain BeH819015.
31238493	4	25	theme	strain	807:812	arg1	BeH819015					814:822	the epidemic strain BeH819015	794:822	the epidemic strain BeH819015	794:822	In the present study, we evaluate the immunogenicity of chimeric viral clone ZIKBeHMR-2, in which the region encoding the structural proteins of the African strain MR766 backbone was replaced with its counterpart from the epidemic strain BeH819015.
31238493	4	26	theme	MR766	740:744	arg1	backbone					746:753	the African strain MR766 backbone	721:753	the African strain MR766 backbone	721:753	In the present study, we evaluate the immunogenicity of chimeric viral clone ZIKBeHMR-2, in which the region encoding the structural proteins of the African strain MR766 backbone was replaced with its counterpart from the epidemic strain BeH819015.
31238493	8	27	theme	anti-ZIKBeHMR-2	1416:1430	arg1	sera					1439:1442	anti-ZIKBeHMR-2 immune sera	1416:1442	anti-ZIKBeHMR-2 immune sera	1416:1442	Antibody-mediated ZIKV neutralization was effective against viral strain MR766, whereas epidemic ZIKV strains were poorly sensitive to neutralization by anti-ZIKBeHMR-2 immune sera.
31238493	2	28	theme	prophylactic	361:372	arg1	treatments					389:398	no specific prophylactic or therapeutic treatments	349:398	no specific prophylactic or therapeutic treatments	349:398	ZIKV infection is recognized as the cause of congenital Zika disease and other neurological defects, with no specific prophylactic or therapeutic treatments.
31238493	0	29	theme	Chimeric	2:9	arg1	Virus					16:20	A Chimeric Zika Virus	0:20	A Chimeric Zika Virus between Viral Strains MR766 and BeH819015	0:62	A Chimeric Zika Virus between Viral Strains MR766 and BeH819015 Highlights a Role for E-glycan Loop in Antibody-mediated Virus Neutralization.
31238493	3	30	theme	urgent	494:499	arg1	need					501:504	an urgent need	491:504	an urgent need for effective vaccines that confer protection against ZIKV infection	491:573	As the humoral immune response is an essential component of protective immunity, there is an urgent need for effective vaccines that confer protection against ZIKV infection.
31238493	2	31	theme	congenital	288:297	arg1	disease					304:310	congenital Zika disease	288:310	congenital Zika disease	288:310	ZIKV infection is recognized as the cause of congenital Zika disease and other neurological defects, with no specific prophylactic or therapeutic treatments.
31238493	2	32	theme	specific	352:359	arg1	treatments					389:398	no specific prophylactic or therapeutic treatments	349:398	no specific prophylactic or therapeutic treatments	349:398	ZIKV infection is recognized as the cause of congenital Zika disease and other neurological defects, with no specific prophylactic or therapeutic treatments.
31238493	5	33	gly	non-glycosylated	959:974	arg1	virus					976:980	a non-glycosylated virus	957:980	ZIKBeHMR-2 a non-glycosylated virus	946:980	Three amino-acid substitutions I152T, T156I, and H158Y were introduced in the glycan loop of the E protein (E-GL) making ZIKBeHMR-2 a non-glycosylated virus.
31238493	5	34	theme	amino-acid	831:840	arg1	substitutions					842:854	Three amino-acid substitutions	825:854	Three amino-acid substitutions I152T, T156I, and H158Y	825:878	Three amino-acid substitutions I152T, T156I, and H158Y were introduced in the glycan loop of the E protein (E-GL) making ZIKBeHMR-2 a non-glycosylated virus.
31238493	5	34	theme	amino-acid	831:840	arg1	T156I					863:867	T156I	863:867	T156I	863:867	Three amino-acid substitutions I152T, T156I, and H158Y were introduced in the glycan loop of the E protein (E-GL) making ZIKBeHMR-2 a non-glycosylated virus.
31238493	5	34	theme	amino-acid	831:840	arg1	H158Y					874:878	H158Y	874:878	H158Y	874:878	Three amino-acid substitutions I152T, T156I, and H158Y were introduced in the glycan loop of the E protein (E-GL) making ZIKBeHMR-2 a non-glycosylated virus.
31238493	5	34	theme	amino-acid	831:840	arg1	I152T					856:860	I152T	856:860	I152T	856:860	Three amino-acid substitutions I152T, T156I, and H158Y were introduced in the glycan loop of the E protein (E-GL) making ZIKBeHMR-2 a non-glycosylated virus.
31238493	4	35	theme	structural	698:707	arg1	proteins					709:716	the structural proteins	694:716	the structural proteins of the African strain MR766 backbone	694:753	In the present study, we evaluate the immunogenicity of chimeric viral clone ZIKBeHMR-2, in which the region encoding the structural proteins of the African strain MR766 backbone was replaced with its counterpart from the epidemic strain BeH819015.
31238493	3	36	theme	humoral	408:414	arg1	response					423:430	the humoral immune response	404:430	the humoral immune response	404:430	As the humoral immune response is an essential component of protective immunity, there is an urgent need for effective vaccines that confer protection against ZIKV infection.
31238493	3	36	theme	humoral	408:414	arg1	component					448:456	an essential component	435:456	an essential component of protective immunity	435:479	As the humoral immune response is an essential component of protective immunity, there is an urgent need for effective vaccines that confer protection against ZIKV infection.
31238493	0	37	theme	Antibody-mediated	103:119	arg1	Neutralization					127:140	Antibody-mediated Virus Neutralization	103:140	Antibody-mediated Virus Neutralization	103:140	A Chimeric Zika Virus between Viral Strains MR766 and BeH819015 Highlights a Role for E-glycan Loop in Antibody-mediated Virus Neutralization.
31238493	8	38	theme	immune	1432:1437	arg1	sera					1439:1442	anti-ZIKBeHMR-2 immune sera	1416:1442	anti-ZIKBeHMR-2 immune sera	1416:1442	Antibody-mediated ZIKV neutralization was effective against viral strain MR766, whereas epidemic ZIKV strains were poorly sensitive to neutralization by anti-ZIKBeHMR-2 immune sera.
31238493	9	39	theme	antibody	1589:1596	arg1	epitopes					1598:1605	neutralizing antibody epitopes	1576:1605	neutralizing antibody epitopes	1576:1605	From our data, we propose that the three E-GL residues at positions E-152, E-156, and E-158 greatly influence the accessibility of neutralizing antibody epitopes on ZIKV.
31238493	7	40	with	Immunization	1165:1176	arg1	ZIKBeHMR-2					1183:1192	ZIKBeHMR-2	1183:1192	ZIKBeHMR-2	1183:1192	Immunization with ZIKBeHMR-2 resulted in a rapid production of neutralizing anti-ZIKV antibodies.
31238493	1	41	theme	emerging	167:174	arg1	virus					148:152	Zika virus	143:152	Zika virus (ZIKV)	143:159	Zika virus (ZIKV) is an emerging mosquito-borne flavivirus which is of major public health concern.
31238493	1	41	theme	emerging	167:174	arg1	flavivirus					191:200	an emerging mosquito-borne flavivirus	164:200	an emerging mosquito-borne flavivirus which is of major public health concern	164:240	Zika virus (ZIKV) is an emerging mosquito-borne flavivirus which is of major public health concern.
31238493	0	42	theme	Zika	11:14	arg1	Virus					16:20	A Chimeric Zika Virus	0:20	A Chimeric Zika Virus between Viral Strains MR766 and BeH819015	0:62	A Chimeric Zika Virus between Viral Strains MR766 and BeH819015 Highlights a Role for E-glycan Loop in Antibody-mediated Virus Neutralization.
31238493	6	43	theme	antibody	1148:1155	arg1	titers					1157:1162	antibody titers	1148:1162	antibody titers	1148:1162	Adult BALB/c mice inoculated intraperitoneally with ZIKBeHMR-2 developed anti-ZIKV antibodies directed against viral proteins E and NS1 and a booster dose increased antibody titers.
31238493	7	44	theme	neutralizing	1228:1239	arg1	antibodies					1251:1260	neutralizing anti-ZIKV antibodies	1228:1260	neutralizing anti-ZIKV antibodies	1228:1260	Immunization with ZIKBeHMR-2 resulted in a rapid production of neutralizing anti-ZIKV antibodies.
31238493	1	45	theme	mosquito-borne	176:189	arg1	virus					148:152	Zika virus	143:152	Zika virus (ZIKV)	143:159	Zika virus (ZIKV) is an emerging mosquito-borne flavivirus which is of major public health concern.
31238493	1	45	theme	mosquito-borne	176:189	arg1	flavivirus					191:200	an emerging mosquito-borne flavivirus	164:200	an emerging mosquito-borne flavivirus which is of major public health concern	164:240	Zika virus (ZIKV) is an emerging mosquito-borne flavivirus which is of major public health concern.
31238493	0	46	theme	Viral	30:34	arg1	Strains					36:42	Viral Strains	30:42	Viral Strains MR766 and BeH819015	30:62	A Chimeric Zika Virus between Viral Strains MR766 and BeH819015 Highlights a Role for E-glycan Loop in Antibody-mediated Virus Neutralization.
31238493	0	46	theme	Viral	30:34	arg1	BeH819015					54:62	BeH819015	54:62	BeH819015	54:62	A Chimeric Zika Virus between Viral Strains MR766 and BeH819015 Highlights a Role for E-glycan Loop in Antibody-mediated Virus Neutralization.
31238493	0	46	theme	Viral	30:34	arg1	MR766					44:48	MR766	44:48	MR766	44:48	A Chimeric Zika Virus between Viral Strains MR766 and BeH819015 Highlights a Role for E-glycan Loop in Antibody-mediated Virus Neutralization.
31238493	5	47	theme	E	922:922	arg1	protein					924:930	the E protein	918:930	the E protein (E-GL) making ZIKBeHMR-2 a non-glycosylated virus	918:980	Three amino-acid substitutions I152T, T156I, and H158Y were introduced in the glycan loop of the E protein (E-GL) making ZIKBeHMR-2 a non-glycosylated virus.
31238493	5	47	theme	E	922:922	arg1	E-GL					933:936	E-GL	933:936	E-GL	933:936	Three amino-acid substitutions I152T, T156I, and H158Y were introduced in the glycan loop of the E protein (E-GL) making ZIKBeHMR-2 a non-glycosylated virus.
31238493	7	48	theme	anti-ZIKV	1241:1249	arg1	antibodies					1251:1260	neutralizing anti-ZIKV antibodies	1228:1260	neutralizing anti-ZIKV antibodies	1228:1260	Immunization with ZIKBeHMR-2 resulted in a rapid production of neutralizing anti-ZIKV antibodies.
31238493	5	49	theme	non-glycosylated	959:974	arg1	virus					976:980	a non-glycosylated virus	957:980	ZIKBeHMR-2 a non-glycosylated virus	946:980	Three amino-acid substitutions I152T, T156I, and H158Y were introduced in the glycan loop of the E protein (E-GL) making ZIKBeHMR-2 a non-glycosylated virus.
31238493	4	50	theme	viral	641:645	arg1	ZIKBeHMR-2					653:662	chimeric viral clone ZIKBeHMR-2	632:662	chimeric viral clone ZIKBeHMR-2	632:662	In the present study, we evaluate the immunogenicity of chimeric viral clone ZIKBeHMR-2, in which the region encoding the structural proteins of the African strain MR766 backbone was replaced with its counterpart from the epidemic strain BeH819015.
31238493	9	51	theme	E-GL	1486:1489	arg1	residues					1491:1498	the three E-GL residues	1476:1498	the three E-GL residues at positions E-152, E-156, and E-158	1476:1535	From our data, we propose that the three E-GL residues at positions E-152, E-156, and E-158 greatly influence the accessibility of neutralizing antibody epitopes on ZIKV.
31238493	4	52	theme	chimeric	632:639	arg1	ZIKBeHMR-2					653:662	chimeric viral clone ZIKBeHMR-2	632:662	chimeric viral clone ZIKBeHMR-2	632:662	In the present study, we evaluate the immunogenicity of chimeric viral clone ZIKBeHMR-2, in which the region encoding the structural proteins of the African strain MR766 backbone was replaced with its counterpart from the epidemic strain BeH819015.
31238493	0	53	theme	Virus	121:125	arg1	Neutralization					127:140	Antibody-mediated Virus Neutralization	103:140	Antibody-mediated Virus Neutralization	103:140	A Chimeric Zika Virus between Viral Strains MR766 and BeH819015 Highlights a Role for E-glycan Loop in Antibody-mediated Virus Neutralization.
31238493	0	54	dep	Strains	36:42	arg1	Strains					36:42	Viral Strains	30:42	Viral Strains MR766 and BeH819015	30:62	A Chimeric Zika Virus between Viral Strains MR766 and BeH819015 Highlights a Role for E-glycan Loop in Antibody-mediated Virus Neutralization.
31238493	0	54	dep	Strains	36:42	arg1	BeH819015					54:62	BeH819015	54:62	BeH819015	54:62	A Chimeric Zika Virus between Viral Strains MR766 and BeH819015 Highlights a Role for E-glycan Loop in Antibody-mediated Virus Neutralization.
31238493	0	54	dep	Strains	36:42	arg1	MR766					44:48	MR766	44:48	MR766	44:48	A Chimeric Zika Virus between Viral Strains MR766 and BeH819015 Highlights a Role for E-glycan Loop in Antibody-mediated Virus Neutralization.
31238493	2	55	theme	therapeutic	377:387	arg1	treatments					389:398	no specific prophylactic or therapeutic treatments	349:398	no specific prophylactic or therapeutic treatments	349:398	ZIKV infection is recognized as the cause of congenital Zika disease and other neurological defects, with no specific prophylactic or therapeutic treatments.
31238493	6	56	theme	BALB/c	989:994	arg1	mice					996:999	Adult BALB/c mice	983:999	Adult BALB/c mice inoculated intraperitoneally with ZIKBeHMR-2	983:1044	Adult BALB/c mice inoculated intraperitoneally with ZIKBeHMR-2 developed anti-ZIKV antibodies directed against viral proteins E and NS1 and a booster dose increased antibody titers.
31238493	3	57	theme	ZIKV	560:563	arg1	infection					565:573	ZIKV infection	560:573	ZIKV infection	560:573	As the humoral immune response is an essential component of protective immunity, there is an urgent need for effective vaccines that confer protection against ZIKV infection.
31238493	2	58	theme	other	316:320	arg1	defects					335:341	other neurological defects	316:341	other neurological defects	316:341	ZIKV infection is recognized as the cause of congenital Zika disease and other neurological defects, with no specific prophylactic or therapeutic treatments.
31238493	5	59	dep	substitutions	842:854	arg1	substitutions					842:854	Three amino-acid substitutions	825:854	Three amino-acid substitutions I152T, T156I, and H158Y	825:878	Three amino-acid substitutions I152T, T156I, and H158Y were introduced in the glycan loop of the E protein (E-GL) making ZIKBeHMR-2 a non-glycosylated virus.
31238493	5	59	dep	substitutions	842:854	arg1	T156I					863:867	T156I	863:867	T156I	863:867	Three amino-acid substitutions I152T, T156I, and H158Y were introduced in the glycan loop of the E protein (E-GL) making ZIKBeHMR-2 a non-glycosylated virus.
31238493	5	59	dep	substitutions	842:854	arg1	H158Y					874:878	H158Y	874:878	H158Y	874:878	Three amino-acid substitutions I152T, T156I, and H158Y were introduced in the glycan loop of the E protein (E-GL) making ZIKBeHMR-2 a non-glycosylated virus.
31238493	5	59	dep	substitutions	842:854	arg1	I152T					856:860	I152T	856:860	I152T	856:860	Three amino-acid substitutions I152T, T156I, and H158Y were introduced in the glycan loop of the E protein (E-GL) making ZIKBeHMR-2 a non-glycosylated virus.
31238493	9	60	theme	neutralizing	1576:1587	arg1	epitopes					1598:1605	neutralizing antibody epitopes	1576:1605	neutralizing antibody epitopes	1576:1605	From our data, we propose that the three E-GL residues at positions E-152, E-156, and E-158 greatly influence the accessibility of neutralizing antibody epitopes on ZIKV.
31238493	7	61	theme	antibodies	1251:1260	arg1	production					1214:1223	a rapid production	1206:1223	a rapid production of neutralizing anti-ZIKV antibodies	1206:1260	Immunization with ZIKBeHMR-2 resulted in a rapid production of neutralizing anti-ZIKV antibodies.
31238493	2	62	theme	ZIKV	243:246	arg1	cause					279:283	the cause	275:283	the cause of congenital Zika disease and other neurological defects	275:341	ZIKV infection is recognized as the cause of congenital Zika disease and other neurological defects, with no specific prophylactic or therapeutic treatments.
31238493	2	62	theme	ZIKV	243:246	arg1	infection					248:256	ZIKV infection	243:256	ZIKV infection	243:256	ZIKV infection is recognized as the cause of congenital Zika disease and other neurological defects, with no specific prophylactic or therapeutic treatments.
31238493	4	63	theme	ZIKBeHMR-2	653:662	arg1	immunogenicity					614:627	the immunogenicity	610:627	the immunogenicity of chimeric viral clone ZIKBeHMR-2, in which the region encoding the structural proteins of the African strain MR766 backbone was replaced with its counterpart from the epidemic strain BeH819015	610:822	In the present study, we evaluate the immunogenicity of chimeric viral clone ZIKBeHMR-2, in which the region encoding the structural proteins of the African strain MR766 backbone was replaced with its counterpart from the epidemic strain BeH819015.
31238493	2	64	theme	disease	304:310	arg1	cause					279:283	the cause	275:283	the cause of congenital Zika disease and other neurological defects	275:341	ZIKV infection is recognized as the cause of congenital Zika disease and other neurological defects, with no specific prophylactic or therapeutic treatments.
31238493	2	64	theme	disease	304:310	arg1	infection					248:256	ZIKV infection	243:256	ZIKV infection	243:256	ZIKV infection is recognized as the cause of congenital Zika disease and other neurological defects, with no specific prophylactic or therapeutic treatments.
31238493	4	65	theme	present	583:589	arg1	study					591:595	the present study	579:595	the present study	579:595	In the present study, we evaluate the immunogenicity of chimeric viral clone ZIKBeHMR-2, in which the region encoding the structural proteins of the African strain MR766 backbone was replaced with its counterpart from the epidemic strain BeH819015.
31238493	4	66	theme	strain	733:738	arg1	backbone					746:753	the African strain MR766 backbone	721:753	the African strain MR766 backbone	721:753	In the present study, we evaluate the immunogenicity of chimeric viral clone ZIKBeHMR-2, in which the region encoding the structural proteins of the African strain MR766 backbone was replaced with its counterpart from the epidemic strain BeH819015.
31238493	2	67	theme	Zika	299:302	arg1	disease					304:310	congenital Zika disease	288:310	congenital Zika disease	288:310	ZIKV infection is recognized as the cause of congenital Zika disease and other neurological defects, with no specific prophylactic or therapeutic treatments.
31238493	8	68	theme	epidemic	1351:1358	arg1	strains					1365:1371	epidemic ZIKV strains	1351:1371	epidemic ZIKV strains	1351:1371	Antibody-mediated ZIKV neutralization was effective against viral strain MR766, whereas epidemic ZIKV strains were poorly sensitive to neutralization by anti-ZIKBeHMR-2 immune sera.
31238493	8	69	theme	viral	1323:1327	arg1	strain					1329:1334	viral strain MR766	1323:1340	viral strain MR766	1323:1340	Antibody-mediated ZIKV neutralization was effective against viral strain MR766, whereas epidemic ZIKV strains were poorly sensitive to neutralization by anti-ZIKBeHMR-2 immune sera.
31238493	7	70	theme	rapid	1208:1212	arg1	production					1214:1223	a rapid production	1206:1223	a rapid production of neutralizing anti-ZIKV antibodies	1206:1260	Immunization with ZIKBeHMR-2 resulted in a rapid production of neutralizing anti-ZIKV antibodies.
31238493	0	71	from	Role	77:80	arg1	Neutralization					127:140	Antibody-mediated Virus Neutralization	103:140	Antibody-mediated Virus Neutralization	103:140	A Chimeric Zika Virus between Viral Strains MR766 and BeH819015 Highlights a Role for E-glycan Loop in Antibody-mediated Virus Neutralization.
31238493	8	72	theme	ZIKV	1281:1284	arg1	neutralization					1286:1299	Antibody-mediated ZIKV neutralization	1263:1299	Antibody-mediated ZIKV neutralization	1263:1299	Antibody-mediated ZIKV neutralization was effective against viral strain MR766, whereas epidemic ZIKV strains were poorly sensitive to neutralization by anti-ZIKBeHMR-2 immune sera.
31238493	3	73	theme	essential	438:446	arg1	response					423:430	the humoral immune response	404:430	the humoral immune response	404:430	As the humoral immune response is an essential component of protective immunity, there is an urgent need for effective vaccines that confer protection against ZIKV infection.
31238493	3	73	theme	essential	438:446	arg1	component					448:456	an essential component	435:456	an essential component of protective immunity	435:479	As the humoral immune response is an essential component of protective immunity, there is an urgent need for effective vaccines that confer protection against ZIKV infection.
31238493	6	74	theme	viral	1094:1098	arg1	E					1109:1109	E	1109:1109	E	1109:1109	Adult BALB/c mice inoculated intraperitoneally with ZIKBeHMR-2 developed anti-ZIKV antibodies directed against viral proteins E and NS1 and a booster dose increased antibody titers.
31238493	6	74	theme	viral	1094:1098	arg1	proteins					1100:1107	viral proteins E and NS1	1094:1117	viral proteins E and NS1	1094:1117	Adult BALB/c mice inoculated intraperitoneally with ZIKBeHMR-2 developed anti-ZIKV antibodies directed against viral proteins E and NS1 and a booster dose increased antibody titers.
31238493	6	74	theme	viral	1094:1098	arg1	NS1					1115:1117	NS1	1115:1117	NS1	1115:1117	Adult BALB/c mice inoculated intraperitoneally with ZIKBeHMR-2 developed anti-ZIKV antibodies directed against viral proteins E and NS1 and a booster dose increased antibody titers.
31238493	1	75	theme	major	214:218	arg1	concern					234:240	major public health concern	214:240	major public health concern	214:240	Zika virus (ZIKV) is an emerging mosquito-borne flavivirus which is of major public health concern.
31238493	1	76	theme	Zika	143:146	arg1	flavivirus					191:200	an emerging mosquito-borne flavivirus	164:200	an emerging mosquito-borne flavivirus which is of major public health concern	164:240	Zika virus (ZIKV) is an emerging mosquito-borne flavivirus which is of major public health concern.
31238493	1	76	theme	Zika	143:146	arg1	ZIKV					155:158	ZIKV	155:158	ZIKV	155:158	Zika virus (ZIKV) is an emerging mosquito-borne flavivirus which is of major public health concern.
31238493	1	76	theme	Zika	143:146	arg1	virus					148:152	Zika virus	143:152	Zika virus (ZIKV)	143:159	Zika virus (ZIKV) is an emerging mosquito-borne flavivirus which is of major public health concern.
31238493	1	77	theme	public	220:225	arg1	concern					234:240	major public health concern	214:240	major public health concern	214:240	Zika virus (ZIKV) is an emerging mosquito-borne flavivirus which is of major public health concern.
31017374	2	0	theme	electrospun	364:374	arg1	membrane					386:393	a drug-loaded double-layered electrospun nanofiber membrane	335:393	a drug-loaded double-layered electrospun nanofiber membrane to prevent the occurrence of epidural adhesion	335:440	In this study, we report a drug-loaded double-layered electrospun nanofiber membrane to prevent the occurrence of epidural adhesion.
31017374	8	1	theme	adhesion	1087:1094	arg1	formation					1096:1104	the epidural adhesion formation	1074:1104	the epidural adhesion formation based on the adhesion scores, histological and biochemical evaluations	1074:1175	In a rabbit laminectomy model, the double-layered membrane can effectively prevent the epidural adhesion formation based on the adhesion scores, histological and biochemical evaluations.
31017374	9	2	theme	inflammation	1240:1251	arg1	reaction					1253:1260	the inflammation reaction	1236:1260	the inflammation reaction	1236:1260	The combination release of MX and MMC can signally reduce the inflammation reaction and collagen I/III expression relative to the case with the membranes loaded with only either one type of the drugs.
31017374	2	3	theme	double-layered	349:362	arg1	membrane					386:393	a drug-loaded double-layered electrospun nanofiber membrane	335:393	a drug-loaded double-layered electrospun nanofiber membrane to prevent the occurrence of epidural adhesion	335:440	In this study, we report a drug-loaded double-layered electrospun nanofiber membrane to prevent the occurrence of epidural adhesion.
31017374	10	4	theme	drug	1436:1439	arg1	system					1450:1455	dual drug delivery system	1431:1455	dual drug delivery system	1431:1455	This approach offers new progresses in constructing dual drug delivery system and provides innovative barrier strategy in inhibiting epidural adhesion post-laminectomy.
31017374	9	5	with	case	1308:1311	arg1	membranes					1322:1330	the membranes	1318:1330	the membranes loaded with only either one type of the drugs	1318:1376	The combination release of MX and MMC can signally reduce the inflammation reaction and collagen I/III expression relative to the case with the membranes loaded with only either one type of the drugs.
31017374	10	6	theme	epidural	1512:1519	arg1	post-laminectomy					1530:1545	epidural adhesion post-laminectomy	1512:1545	epidural adhesion post-laminectomy	1512:1545	This approach offers new progresses in constructing dual drug delivery system and provides innovative barrier strategy in inhibiting epidural adhesion post-laminectomy.
31017374	1	7	theme	nerve	275:279	arg1	compression					281:291	nerve compression	275:291	nerve compression	275:291	Epidural adhesion between the spinal dura and the surrounding fibrous tissue often occurs post-laminectomy, resulting in clinical symptoms such as nerve compression and severe pain.
31017374	9	8	theme	drugs	1372:1376	arg1	type					1360:1363	only either one type	1344:1363	only either one type of the drugs	1344:1376	The combination release of MX and MMC can signally reduce the inflammation reaction and collagen I/III expression relative to the case with the membranes loaded with only either one type of the drugs.
31017374	0	9	theme	adhesions	117:125	arg1	prevention					94:103	the prevention	90:103	the prevention of epidural adhesions	90:125	Effective delivery of mitomycin-C and meloxicam by double-layer electrospun membranes for the prevention of epidural adhesions.
31017374	6	10	theme	drugs	818:822	arg1	types					809:813	The two types	801:813	The two types of drugs	801:822	The two types of drugs are released from the double-layered membrane within about 12 days.
31017374	8	11	theme	adhesion	1119:1126	arg1	evaluations					1165:1175	histological and biochemical evaluations	1136:1175	histological and biochemical evaluations	1136:1175	In a rabbit laminectomy model, the double-layered membrane can effectively prevent the epidural adhesion formation based on the adhesion scores, histological and biochemical evaluations.
31017374	8	11	theme	adhesion	1119:1126	arg1	scores					1128:1133	the adhesion scores	1115:1133	the adhesion scores	1115:1133	In a rabbit laminectomy model, the double-layered membrane can effectively prevent the epidural adhesion formation based on the adhesion scores, histological and biochemical evaluations.
31017374	4	12	theme	spinal	606:611	arg1	dura					613:616	the spinal dura	602:616	the spinal dura	602:616	The bottom layer contacting to the spinal dura is loaded with meloxicam (MX) to prevent inflammation.
31017374	3	13	theme	chitosan	525:532	arg1	mixture					487:493	a mixture	485:493	a mixture of polycaprolactone (PCL) and chitosan (CS)	485:537	The nanofibers in both layers are made of a mixture of polycaprolactone (PCL) and chitosan (CS) but at different weight ratios.
31017374	5	14	theme	collagen	791:798	arg1	synthesis					770:778	the synthesis	766:778	the synthesis of DNA and collagen	766:798	The top layer that contacts to the fibrous tissue is doped with mitomycin-C (MMC) to inhibit the synthesis of DNA and collagen.
31017374	0	15	theme	epidural	108:115	arg1	adhesions					117:125	epidural adhesions	108:125	epidural adhesions	108:125	Effective delivery of mitomycin-C and meloxicam by double-layer electrospun membranes for the prevention of epidural adhesions.
31017374	9	16	theme	I/III	1275:1279	arg1	expression					1281:1290	collagen I/III expression	1266:1290	collagen I/III expression	1266:1290	The combination release of MX and MMC can signally reduce the inflammation reaction and collagen I/III expression relative to the case with the membranes loaded with only either one type of the drugs.
31017374	1	17	theme	surrounding	178:188	arg1	tissue					198:203	the surrounding fibrous tissue	174:203	the surrounding fibrous tissue	174:203	Epidural adhesion between the spinal dura and the surrounding fibrous tissue often occurs post-laminectomy, resulting in clinical symptoms such as nerve compression and severe pain.
31017374	8	18	theme	laminectomy	1003:1013	arg1	model					1015:1019	a rabbit laminectomy model	994:1019	a rabbit laminectomy model	994:1019	In a rabbit laminectomy model, the double-layered membrane can effectively prevent the epidural adhesion formation based on the adhesion scores, histological and biochemical evaluations.
31017374	8	19	theme	epidural	1078:1085	arg1	formation					1096:1104	the epidural adhesion formation	1074:1104	the epidural adhesion formation based on the adhesion scores, histological and biochemical evaluations	1074:1175	In a rabbit laminectomy model, the double-layered membrane can effectively prevent the epidural adhesion formation based on the adhesion scores, histological and biochemical evaluations.
31017374	3	20	theme	different	546:554	arg1	ratios					563:568	different weight ratios	546:568	different weight ratios	546:568	The nanofibers in both layers are made of a mixture of polycaprolactone (PCL) and chitosan (CS) but at different weight ratios.
31017374	9	21	theme	collagen	1266:1273	arg1	expression					1281:1290	collagen I/III expression	1266:1290	collagen I/III expression	1266:1290	The combination release of MX and MMC can signally reduce the inflammation reaction and collagen I/III expression relative to the case with the membranes loaded with only either one type of the drugs.
31017374	1	22	theme	fibrous	190:196	arg1	tissue					198:203	the surrounding fibrous tissue	174:203	the surrounding fibrous tissue	174:203	Epidural adhesion between the spinal dura and the surrounding fibrous tissue often occurs post-laminectomy, resulting in clinical symptoms such as nerve compression and severe pain.
31017374	0	23	theme	Effective	0:8	arg1	delivery					10:17	Effective delivery	0:17	Effective delivery of mitomycin-C and meloxicam by double-layer electrospun membranes for the prevention of epidural adhesions.	0:126	Effective delivery of mitomycin-C and meloxicam by double-layer electrospun membranes for the prevention of epidural adhesions.
31017374	9	24	theme	relative	1292:1299	arg1	reaction					1253:1260	the inflammation reaction	1236:1260	the inflammation reaction	1236:1260	The combination release of MX and MMC can signally reduce the inflammation reaction and collagen I/III expression relative to the case with the membranes loaded with only either one type of the drugs.
31017374	8	25	theme	rabbit	996:1001	arg1	model					1015:1019	a rabbit laminectomy model	994:1019	a rabbit laminectomy model	994:1019	In a rabbit laminectomy model, the double-layered membrane can effectively prevent the epidural adhesion formation based on the adhesion scores, histological and biochemical evaluations.
31017374	3	26	theme	weight	556:561	arg1	ratios					563:568	different weight ratios	546:568	different weight ratios	546:568	The nanofibers in both layers are made of a mixture of polycaprolactone (PCL) and chitosan (CS) but at different weight ratios.
31017374	0	27	theme	mitomycin-C	22:32	arg1	delivery					10:17	Effective delivery	0:17	Effective delivery of mitomycin-C and meloxicam by double-layer electrospun membranes for the prevention of epidural adhesions.	0:126	Effective delivery of mitomycin-C and meloxicam by double-layer electrospun membranes for the prevention of epidural adhesions.
31017374	1	28	theme	severe	297:302	arg1	pain					304:307	severe pain	297:307	severe pain	297:307	Epidural adhesion between the spinal dura and the surrounding fibrous tissue often occurs post-laminectomy, resulting in clinical symptoms such as nerve compression and severe pain.
31017374	10	29	theme	new	1400:1402	arg1	progresses					1404:1413	new progresses	1400:1413	new progresses	1400:1413	This approach offers new progresses in constructing dual drug delivery system and provides innovative barrier strategy in inhibiting epidural adhesion post-laminectomy.
31017374	7	30	theme	fibroblasts	928:938	arg1	proliferation					940:952	fibroblasts proliferation	928:952	fibroblasts proliferation	928:952	Meanwhile, the membrane can inhibit fibroblasts proliferation in vitro while show no cytotoxicity.
31017374	10	31	theme	delivery	1441:1448	arg1	system					1450:1455	dual drug delivery system	1431:1455	dual drug delivery system	1431:1455	This approach offers new progresses in constructing dual drug delivery system and provides innovative barrier strategy in inhibiting epidural adhesion post-laminectomy.
31017374	9	32	theme	combination	1182:1192	arg1	release					1194:1200	The combination release	1178:1200	The combination release of MX and MMC	1178:1214	The combination release of MX and MMC can signally reduce the inflammation reaction and collagen I/III expression relative to the case with the membranes loaded with only either one type of the drugs.
31017374	2	33	theme	adhesion	433:440	arg1	occurrence					410:419	the occurrence	406:419	the occurrence of epidural adhesion	406:440	In this study, we report a drug-loaded double-layered electrospun nanofiber membrane to prevent the occurrence of epidural adhesion.
31017374	0	34	theme	meloxicam	38:46	arg1	delivery					10:17	Effective delivery	0:17	Effective delivery of mitomycin-C and meloxicam by double-layer electrospun membranes for the prevention of epidural adhesions.	0:126	Effective delivery of mitomycin-C and meloxicam by double-layer electrospun membranes for the prevention of epidural adhesions.
31017374	3	35	theme	polycaprolactone	498:513	arg1	mixture					487:493	a mixture	485:493	a mixture of polycaprolactone (PCL) and chitosan (CS)	485:537	The nanofibers in both layers are made of a mixture of polycaprolactone (PCL) and chitosan (CS) but at different weight ratios.
31017374	5	36	theme	top	677:679	arg1	layer					681:685	The top layer	673:685	The top layer that contacts to the fibrous tissue	673:721	The top layer that contacts to the fibrous tissue is doped with mitomycin-C (MMC) to inhibit the synthesis of DNA and collagen.
31017374	5	37	theme	DNA	783:785	arg1	synthesis					770:778	the synthesis	766:778	the synthesis of DNA and collagen	766:798	The top layer that contacts to the fibrous tissue is doped with mitomycin-C (MMC) to inhibit the synthesis of DNA and collagen.
31017374	2	38	theme	epidural	424:431	arg1	adhesion					433:440	epidural adhesion	424:440	epidural adhesion	424:440	In this study, we report a drug-loaded double-layered electrospun nanofiber membrane to prevent the occurrence of epidural adhesion.
31017374	8	39	theme	biochemical	1153:1163	arg1	evaluations					1165:1175	histological and biochemical evaluations	1136:1175	histological and biochemical evaluations	1136:1175	In a rabbit laminectomy model, the double-layered membrane can effectively prevent the epidural adhesion formation based on the adhesion scores, histological and biochemical evaluations.
31017374	8	39	theme	biochemical	1153:1163	arg1	scores					1128:1133	the adhesion scores	1115:1133	the adhesion scores	1115:1133	In a rabbit laminectomy model, the double-layered membrane can effectively prevent the epidural adhesion formation based on the adhesion scores, histological and biochemical evaluations.
31017374	10	40	theme	dual	1431:1434	arg1	system					1450:1455	dual drug delivery system	1431:1455	dual drug delivery system	1431:1455	This approach offers new progresses in constructing dual drug delivery system and provides innovative barrier strategy in inhibiting epidural adhesion post-laminectomy.
31017374	10	41	theme	adhesion	1521:1528	arg1	post-laminectomy					1530:1545	epidural adhesion post-laminectomy	1512:1545	epidural adhesion post-laminectomy	1512:1545	This approach offers new progresses in constructing dual drug delivery system and provides innovative barrier strategy in inhibiting epidural adhesion post-laminectomy.
31017374	0	42	theme	double-layer	51:62	arg1	membranes					76:84	double-layer electrospun membranes	51:84	double-layer electrospun membranes for the prevention of epidural adhesions	51:125	Effective delivery of mitomycin-C and meloxicam by double-layer electrospun membranes for the prevention of epidural adhesions.
31017374	6	43	theme	about	877:881	arg1	12 days					883:889	about 12 days	877:889	about 12 days	877:889	The two types of drugs are released from the double-layered membrane within about 12 days.
31017374	10	44	theme	barrier	1481:1487	arg1	strategy					1489:1496	innovative barrier strategy	1470:1496	innovative barrier strategy	1470:1496	This approach offers new progresses in constructing dual drug delivery system and provides innovative barrier strategy in inhibiting epidural adhesion post-laminectomy.
31017374	9	45	theme	MX	1205:1206	arg1	release					1194:1200	The combination release	1178:1200	The combination release of MX and MMC	1178:1214	The combination release of MX and MMC can signally reduce the inflammation reaction and collagen I/III expression relative to the case with the membranes loaded with only either one type of the drugs.
31017374	2	46	theme	drug-loaded	337:347	arg1	membrane					386:393	a drug-loaded double-layered electrospun nanofiber membrane	335:393	a drug-loaded double-layered electrospun nanofiber membrane to prevent the occurrence of epidural adhesion	335:440	In this study, we report a drug-loaded double-layered electrospun nanofiber membrane to prevent the occurrence of epidural adhesion.
31017374	10	47	theme	innovative	1470:1479	arg1	strategy					1489:1496	innovative barrier strategy	1470:1496	innovative barrier strategy	1470:1496	This approach offers new progresses in constructing dual drug delivery system and provides innovative barrier strategy in inhibiting epidural adhesion post-laminectomy.
31017374	4	48	theme	bottom	575:580	arg1	layer					582:586	The bottom layer	571:586	The bottom layer contacting to the spinal dura	571:616	The bottom layer contacting to the spinal dura is loaded with meloxicam (MX) to prevent inflammation.
31017374	1	49	theme	Epidural	128:135	arg1	adhesion					137:144	Epidural adhesion	128:144	Epidural adhesion between the spinal dura and the surrounding fibrous tissue	128:203	Epidural adhesion between the spinal dura and the surrounding fibrous tissue often occurs post-laminectomy, resulting in clinical symptoms such as nerve compression and severe pain.
31017374	6	50	attach	released	828:835	arg2	types					809:813	The two types	801:813	The two types of drugs	801:822	The two types of drugs are released from the double-layered membrane within about 12 days.
31017374	6	50	attach	released	828:835	arg1	membrane					861:868	the double-layered membrane	842:868	the double-layered membrane	842:868	The two types of drugs are released from the double-layered membrane within about 12 days.
31017374	8	51	theme	double-layered	1026:1039	arg1	membrane					1041:1048	the double-layered membrane	1022:1048	the double-layered membrane	1022:1048	In a rabbit laminectomy model, the double-layered membrane can effectively prevent the epidural adhesion formation based on the adhesion scores, histological and biochemical evaluations.
31017374	9	52	theme	MMC	1212:1214	arg1	release					1194:1200	The combination release	1178:1200	The combination release of MX and MMC	1178:1214	The combination release of MX and MMC can signally reduce the inflammation reaction and collagen I/III expression relative to the case with the membranes loaded with only either one type of the drugs.
31017374	1	53	theme	spinal	158:163	arg1	dura					165:168	the spinal dura	154:168	the spinal dura	154:168	Epidural adhesion between the spinal dura and the surrounding fibrous tissue often occurs post-laminectomy, resulting in clinical symptoms such as nerve compression and severe pain.
31017374	5	54	theme	fibrous	708:714	arg1	tissue					716:721	the fibrous tissue	704:721	the fibrous tissue	704:721	The top layer that contacts to the fibrous tissue is doped with mitomycin-C (MMC) to inhibit the synthesis of DNA and collagen.
31017374	3	55	from	nanofibers	447:456	arg1	layers					466:471	both layers	461:471	both layers	461:471	The nanofibers in both layers are made of a mixture of polycaprolactone (PCL) and chitosan (CS) but at different weight ratios.
31017374	1	56	theme	clinical	249:256	arg1	pain					304:307	severe pain	297:307	severe pain	297:307	Epidural adhesion between the spinal dura and the surrounding fibrous tissue often occurs post-laminectomy, resulting in clinical symptoms such as nerve compression and severe pain.
31017374	1	56	theme	clinical	249:256	arg1	symptoms					258:265	clinical symptoms	249:265	clinical symptoms such as nerve compression and severe pain	249:307	Epidural adhesion between the spinal dura and the surrounding fibrous tissue often occurs post-laminectomy, resulting in clinical symptoms such as nerve compression and severe pain.
31017374	1	56	theme	clinical	249:256	arg1	compression					281:291	nerve compression	275:291	nerve compression	275:291	Epidural adhesion between the spinal dura and the surrounding fibrous tissue often occurs post-laminectomy, resulting in clinical symptoms such as nerve compression and severe pain.
31017374	0	57	theme	electrospun	64:74	arg1	membranes					76:84	double-layer electrospun membranes	51:84	double-layer electrospun membranes for the prevention of epidural adhesions	51:125	Effective delivery of mitomycin-C and meloxicam by double-layer electrospun membranes for the prevention of epidural adhesions.
31017374	6	58	theme	double-layered	846:859	arg1	membrane					861:868	the double-layered membrane	842:868	the double-layered membrane	842:868	The two types of drugs are released from the double-layered membrane within about 12 days.
31017374	8	59	theme	histological	1136:1147	arg1	evaluations					1165:1175	histological and biochemical evaluations	1136:1175	histological and biochemical evaluations	1136:1175	In a rabbit laminectomy model, the double-layered membrane can effectively prevent the epidural adhesion formation based on the adhesion scores, histological and biochemical evaluations.
31017374	8	59	theme	histological	1136:1147	arg1	scores					1128:1133	the adhesion scores	1115:1133	the adhesion scores	1115:1133	In a rabbit laminectomy model, the double-layered membrane can effectively prevent the epidural adhesion formation based on the adhesion scores, histological and biochemical evaluations.
31017374	2	60	theme	nanofiber	376:384	arg1	membrane					386:393	a drug-loaded double-layered electrospun nanofiber membrane	335:393	a drug-loaded double-layered electrospun nanofiber membrane to prevent the occurrence of epidural adhesion	335:440	In this study, we report a drug-loaded double-layered electrospun nanofiber membrane to prevent the occurrence of epidural adhesion.
29874067	2	0	theme	glutathione-cleavable	511:531	arg1	bond					543:546	a glutathione-cleavable disulfide bond	509:546	a glutathione-cleavable disulfide bond	509:546	Herein, we innovatively develop a theranostic supramolecular polymer using β-cyclodextrin as the host and camptothecin (CPT) as the guest linked by a glutathione-cleavable disulfide bond.
29874067	4	1	theme	hydrogen	960:967	arg1	bonds					969:973	hydrogen bonds	960:973	hydrogen bonds	960:973	Supramolecular nanoparticles can be prepared through orthogonal self-assembly driven by π-π stacking interaction, host-guest complexation, and hydrogen bonds.
29874067	1	2	theme	past	192:195	arg1	decades					197:203	the past decades	188:203	the past decades	188:203	Nanomedicines have achieved several breakthroughs in cancer treatment over the past decades; however, their potential immunotoxicities are ignored, which results in serious adverse effects and greatly reduces the potential in clinical translation.
29874067	1	3	theme	serious	278:284	arg1	effects					294:300	serious adverse effects	278:300	serious adverse effects	278:300	Nanomedicines have achieved several breakthroughs in cancer treatment over the past decades; however, their potential immunotoxicities are ignored, which results in serious adverse effects and greatly reduces the potential in clinical translation.
29874067	4	4	theme	π-π	905:907	arg1	interaction					918:928	π-π stacking interaction	905:928	π-π stacking interaction	905:928	Supramolecular nanoparticles can be prepared through orthogonal self-assembly driven by π-π stacking interaction, host-guest complexation, and hydrogen bonds.
29874067	1	5	theme	adverse	286:292	arg1	effects					294:300	serious adverse effects	278:300	serious adverse effects	278:300	Nanomedicines have achieved several breakthroughs in cancer treatment over the past decades; however, their potential immunotoxicities are ignored, which results in serious adverse effects and greatly reduces the potential in clinical translation.
29874067	6	6	dep	In	1238:1239	arg1	vivo					1241:1244	vivo	1241:1244	vivo	1241:1244	In vivo investigations demonstrate this supramolecular nanomedicine possesses superior antitumor performance and antimetastasis capability.
29874067	6	7	theme	antimetastasis	1351:1364	arg1	capability					1366:1375	antimetastasis capability	1351:1375	antimetastasis capability	1351:1375	In vivo investigations demonstrate this supramolecular nanomedicine possesses superior antitumor performance and antimetastasis capability.
29874067	3	8	theme	CPT	622:624	arg1	solubility					608:617	the solubility	604:617	the solubility of CPT	604:624	The supramolecular polymerization remarkably increases the solubility of CPT by a factor of 232 and effectively inhibits its lactone ring opening in physiological environment, which is favorable for intravenous formulation and maintenance of the therapeutic efficacy.
29874067	6	9	contain	possesses	1306:1314	arg2	capability					1366:1375	antimetastasis capability	1351:1375	antimetastasis capability	1351:1375	In vivo investigations demonstrate this supramolecular nanomedicine possesses superior antitumor performance and antimetastasis capability.
29874067	6	9	contain	possesses	1306:1314	arg2	performance					1335:1345	superior antitumor performance	1316:1345	superior antitumor performance	1316:1345	In vivo investigations demonstrate this supramolecular nanomedicine possesses superior antitumor performance and antimetastasis capability.
29874067	6	9	contain	possesses	1306:1314	arg1	nanomedicine					1293:1304	this supramolecular nanomedicine	1273:1304	this supramolecular nanomedicine	1273:1304	In vivo investigations demonstrate this supramolecular nanomedicine possesses superior antitumor performance and antimetastasis capability.
29874067	7	10	theme	pioneering	1383:1392	arg1	example					1394:1400	This pioneering example	1378:1400	This pioneering example integrating the advantages of the dynamic nature of supramolecular chemistry and nanotechnology	1378:1496	This pioneering example integrating the advantages of the dynamic nature of supramolecular chemistry and nanotechnology provides a promising platform for cancer theranostics.
29874067	5	11	theme	long-term	1212:1220	arg1	toxicity					1191:1198	systemic toxicity	1182:1198	systemic toxicity	1182:1198	The sophisticated nanomedicine constructed from the obtained supramolecular polymer can be specifically delivered to tumor sites and rapidly excreted from body after drug release, thus effectively avoiding systemic toxicity, especially long-term immunotoxicity.
29874067	5	11	theme	long-term	1212:1220	arg1	immunotoxicity					1222:1235	long-term immunotoxicity	1212:1235	long-term immunotoxicity	1212:1235	The sophisticated nanomedicine constructed from the obtained supramolecular polymer can be specifically delivered to tumor sites and rapidly excreted from body after drug release, thus effectively avoiding systemic toxicity, especially long-term immunotoxicity.
29874067	5	12	theme	supramolecular	1037:1050	arg1	polymer					1052:1058	the obtained supramolecular polymer	1024:1058	the obtained supramolecular polymer	1024:1058	The sophisticated nanomedicine constructed from the obtained supramolecular polymer can be specifically delivered to tumor sites and rapidly excreted from body after drug release, thus effectively avoiding systemic toxicity, especially long-term immunotoxicity.
29874067	7	13	theme	dynamic	1436:1442	arg1	nature					1444:1449	the dynamic nature	1432:1449	the dynamic nature of supramolecular chemistry and nanotechnology	1432:1496	This pioneering example integrating the advantages of the dynamic nature of supramolecular chemistry and nanotechnology provides a promising platform for cancer theranostics.
29874067	4	14	theme	host-guest	931:940	arg1	complexation					942:953	host-guest complexation	931:953	host-guest complexation	931:953	Supramolecular nanoparticles can be prepared through orthogonal self-assembly driven by π-π stacking interaction, host-guest complexation, and hydrogen bonds.
29874067	7	15	theme	promising	1509:1517	arg1	platform					1519:1526	a promising platform	1507:1526	a promising platform for cancer theranostics	1507:1550	This pioneering example integrating the advantages of the dynamic nature of supramolecular chemistry and nanotechnology provides a promising platform for cancer theranostics.
29874067	5	16	theme	tumor	1093:1097	arg1	sites					1099:1103	tumor sites	1093:1103	tumor sites	1093:1103	The sophisticated nanomedicine constructed from the obtained supramolecular polymer can be specifically delivered to tumor sites and rapidly excreted from body after drug release, thus effectively avoiding systemic toxicity, especially long-term immunotoxicity.
29874067	7	17	theme	cancer	1532:1537	arg1	theranostics					1539:1550	cancer theranostics	1532:1550	cancer theranostics	1532:1550	This pioneering example integrating the advantages of the dynamic nature of supramolecular chemistry and nanotechnology provides a promising platform for cancer theranostics.
29874067	1	18	from	potential	326:334	arg1	translation					348:358	clinical translation	339:358	clinical translation	339:358	Nanomedicines have achieved several breakthroughs in cancer treatment over the past decades; however, their potential immunotoxicities are ignored, which results in serious adverse effects and greatly reduces the potential in clinical translation.
29874067	6	19	theme	antitumor	1325:1333	arg1	performance					1335:1345	superior antitumor performance	1316:1345	superior antitumor performance	1316:1345	In vivo investigations demonstrate this supramolecular nanomedicine possesses superior antitumor performance and antimetastasis capability.
29874067	0	20	theme	Polymer-Based	15:27	arg1	Nanomedicine					29:40	Supramolecular Polymer-Based Nanomedicine	0:40	Supramolecular Polymer-Based Nanomedicine	0:40	Supramolecular Polymer-Based Nanomedicine: High Therapeutic Performance and Negligible Long-Term Immunotoxicity.
29874067	6	21	theme	superior	1316:1323	arg1	performance					1335:1345	superior antitumor performance	1316:1345	superior antitumor performance	1316:1345	In vivo investigations demonstrate this supramolecular nanomedicine possesses superior antitumor performance and antimetastasis capability.
29874067	7	22	theme	supramolecular	1454:1467	arg1	chemistry					1469:1477	supramolecular chemistry	1454:1477	supramolecular chemistry	1454:1477	This pioneering example integrating the advantages of the dynamic nature of supramolecular chemistry and nanotechnology provides a promising platform for cancer theranostics.
29874067	0	23	theme	Supramolecular	0:13	arg1	Nanomedicine					29:40	Supramolecular Polymer-Based Nanomedicine	0:40	Supramolecular Polymer-Based Nanomedicine	0:40	Supramolecular Polymer-Based Nanomedicine: High Therapeutic Performance and Negligible Long-Term Immunotoxicity.
29874067	1	24	from	breakthroughs	149:161	arg1	treatment					173:181	cancer treatment	166:181	cancer treatment	166:181	Nanomedicines have achieved several breakthroughs in cancer treatment over the past decades; however, their potential immunotoxicities are ignored, which results in serious adverse effects and greatly reduces the potential in clinical translation.
29874067	6	25	theme	supramolecular	1278:1291	arg1	nanomedicine					1293:1304	this supramolecular nanomedicine	1273:1304	this supramolecular nanomedicine	1273:1304	In vivo investigations demonstrate this supramolecular nanomedicine possesses superior antitumor performance and antimetastasis capability.
29874067	4	26	theme	Supramolecular	817:830	arg1	nanoparticles					832:844	Supramolecular nanoparticles	817:844	Supramolecular nanoparticles	817:844	Supramolecular nanoparticles can be prepared through orthogonal self-assembly driven by π-π stacking interaction, host-guest complexation, and hydrogen bonds.
29874067	3	27	theme	232	641:643	arg1	factor					631:636	a factor	629:636	a factor of 232	629:643	The supramolecular polymerization remarkably increases the solubility of CPT by a factor of 232 and effectively inhibits its lactone ring opening in physiological environment, which is favorable for intravenous formulation and maintenance of the therapeutic efficacy.
29874067	1	28	theme	potential	221:229	arg1	immunotoxicities					231:246	their potential immunotoxicities	215:246	their potential immunotoxicities	215:246	Nanomedicines have achieved several breakthroughs in cancer treatment over the past decades; however, their potential immunotoxicities are ignored, which results in serious adverse effects and greatly reduces the potential in clinical translation.
29874067	3	29	theme	therapeutic	795:805	arg1	efficacy					807:814	the therapeutic efficacy	791:814	the therapeutic efficacy	791:814	The supramolecular polymerization remarkably increases the solubility of CPT by a factor of 232 and effectively inhibits its lactone ring opening in physiological environment, which is favorable for intravenous formulation and maintenance of the therapeutic efficacy.
29874067	5	30	theme	systemic	1182:1189	arg1	toxicity					1191:1198	systemic toxicity	1182:1198	systemic toxicity	1182:1198	The sophisticated nanomedicine constructed from the obtained supramolecular polymer can be specifically delivered to tumor sites and rapidly excreted from body after drug release, thus effectively avoiding systemic toxicity, especially long-term immunotoxicity.
29874067	5	30	theme	systemic	1182:1189	arg1	immunotoxicity					1222:1235	long-term immunotoxicity	1212:1235	long-term immunotoxicity	1212:1235	The sophisticated nanomedicine constructed from the obtained supramolecular polymer can be specifically delivered to tumor sites and rapidly excreted from body after drug release, thus effectively avoiding systemic toxicity, especially long-term immunotoxicity.
29874067	1	31	theme	several	141:147	arg1	breakthroughs					149:161	several breakthroughs	141:161	several breakthroughs in cancer treatment	141:181	Nanomedicines have achieved several breakthroughs in cancer treatment over the past decades; however, their potential immunotoxicities are ignored, which results in serious adverse effects and greatly reduces the potential in clinical translation.
29874067	2	32	theme	supramolecular	407:420	arg1	polymer					422:428	a theranostic supramolecular polymer	393:428	a theranostic supramolecular polymer using β-cyclodextrin as the host and camptothecin (CPT) as the guest linked by a glutathione-cleavable disulfide bond	393:546	Herein, we innovatively develop a theranostic supramolecular polymer using β-cyclodextrin as the host and camptothecin (CPT) as the guest linked by a glutathione-cleavable disulfide bond.
29874067	0	33	theme	Therapeutic	48:58	arg1	Performance					60:70	High Therapeutic Performance	43:70	High Therapeutic Performance	43:70	Supramolecular Polymer-Based Nanomedicine: High Therapeutic Performance and Negligible Long-Term Immunotoxicity.
29874067	3	34	theme	efficacy	807:814	arg1	maintenance					776:786	maintenance	776:786	maintenance	776:786	The supramolecular polymerization remarkably increases the solubility of CPT by a factor of 232 and effectively inhibits its lactone ring opening in physiological environment, which is favorable for intravenous formulation and maintenance of the therapeutic efficacy.
29874067	3	34	theme	efficacy	807:814	arg1	formulation					760:770	formulation	760:770	formulation	760:770	The supramolecular polymerization remarkably increases the solubility of CPT by a factor of 232 and effectively inhibits its lactone ring opening in physiological environment, which is favorable for intravenous formulation and maintenance of the therapeutic efficacy.
29874067	3	35	theme	physiological	698:710	arg1	environment					712:722	physiological environment	698:722	physiological environment	698:722	The supramolecular polymerization remarkably increases the solubility of CPT by a factor of 232 and effectively inhibits its lactone ring opening in physiological environment, which is favorable for intravenous formulation and maintenance of the therapeutic efficacy.
29874067	2	36	dep	host	458:461	arg1	the					454:456	the	454:456	the	454:456	Herein, we innovatively develop a theranostic supramolecular polymer using β-cyclodextrin as the host and camptothecin (CPT) as the guest linked by a glutathione-cleavable disulfide bond.
29874067	7	37	theme	nanotechnology	1483:1496	arg1	nature					1444:1449	the dynamic nature	1432:1449	the dynamic nature of supramolecular chemistry and nanotechnology	1432:1496	This pioneering example integrating the advantages of the dynamic nature of supramolecular chemistry and nanotechnology provides a promising platform for cancer theranostics.
29874067	0	38	theme	High	43:46	arg1	Performance					60:70	High Therapeutic Performance	43:70	High Therapeutic Performance	43:70	Supramolecular Polymer-Based Nanomedicine: High Therapeutic Performance and Negligible Long-Term Immunotoxicity.
29874067	3	39	theme	ring	682:685	arg1	opening					687:693	its lactone ring opening	670:693	its lactone ring opening	670:693	The supramolecular polymerization remarkably increases the solubility of CPT by a factor of 232 and effectively inhibits its lactone ring opening in physiological environment, which is favorable for intravenous formulation and maintenance of the therapeutic efficacy.
29874067	1	40	theme	clinical	339:346	arg1	translation					348:358	clinical translation	339:358	clinical translation	339:358	Nanomedicines have achieved several breakthroughs in cancer treatment over the past decades; however, their potential immunotoxicities are ignored, which results in serious adverse effects and greatly reduces the potential in clinical translation.
29874067	0	41	theme	Negligible	76:85	arg1	Immunotoxicity					97:110	Negligible Long-Term Immunotoxicity	76:110	Negligible Long-Term Immunotoxicity	76:110	Supramolecular Polymer-Based Nanomedicine: High Therapeutic Performance and Negligible Long-Term Immunotoxicity.
29874067	5	42	theme	drug	1142:1145	arg1	release					1147:1153	drug release	1142:1153	drug release	1142:1153	The sophisticated nanomedicine constructed from the obtained supramolecular polymer can be specifically delivered to tumor sites and rapidly excreted from body after drug release, thus effectively avoiding systemic toxicity, especially long-term immunotoxicity.
29874067	6	43	theme	In	1238:1239	arg1	investigations					1246:1259	In vivo investigations	1238:1259	In vivo investigations	1238:1259	In vivo investigations demonstrate this supramolecular nanomedicine possesses superior antitumor performance and antimetastasis capability.
29874067	7	44	theme	chemistry	1469:1477	arg1	nature					1444:1449	the dynamic nature	1432:1449	the dynamic nature of supramolecular chemistry and nanotechnology	1432:1496	This pioneering example integrating the advantages of the dynamic nature of supramolecular chemistry and nanotechnology provides a promising platform for cancer theranostics.
29874067	4	45	theme	stacking	909:916	arg1	interaction					918:928	π-π stacking interaction	905:928	π-π stacking interaction	905:928	Supramolecular nanoparticles can be prepared through orthogonal self-assembly driven by π-π stacking interaction, host-guest complexation, and hydrogen bonds.
29874067	7	46	theme	nature	1444:1449	arg1	advantages					1418:1427	the advantages	1414:1427	the advantages of the dynamic nature of supramolecular chemistry and nanotechnology	1414:1496	This pioneering example integrating the advantages of the dynamic nature of supramolecular chemistry and nanotechnology provides a promising platform for cancer theranostics.
29874067	4	47	theme	orthogonal	870:879	arg1	self-assembly					881:893	orthogonal self-assembly	870:893	orthogonal self-assembly driven by π-π stacking interaction, host-guest complexation, and hydrogen bonds	870:973	Supramolecular nanoparticles can be prepared through orthogonal self-assembly driven by π-π stacking interaction, host-guest complexation, and hydrogen bonds.
29874067	1	48	theme	cancer	166:171	arg1	treatment					173:181	cancer treatment	166:181	cancer treatment	166:181	Nanomedicines have achieved several breakthroughs in cancer treatment over the past decades; however, their potential immunotoxicities are ignored, which results in serious adverse effects and greatly reduces the potential in clinical translation.
29874067	3	49	theme	lactone	674:680	arg1	opening					687:693	its lactone ring opening	670:693	its lactone ring opening	670:693	The supramolecular polymerization remarkably increases the solubility of CPT by a factor of 232 and effectively inhibits its lactone ring opening in physiological environment, which is favorable for intravenous formulation and maintenance of the therapeutic efficacy.
29874067	5	50	theme	sophisticated	980:992	arg1	nanomedicine					994:1005	The sophisticated nanomedicine	976:1005	The sophisticated nanomedicine constructed from the obtained supramolecular polymer	976:1058	The sophisticated nanomedicine constructed from the obtained supramolecular polymer can be specifically delivered to tumor sites and rapidly excreted from body after drug release, thus effectively avoiding systemic toxicity, especially long-term immunotoxicity.
29874067	5	51	theme	obtained	1028:1035	arg1	polymer					1052:1058	the obtained supramolecular polymer	1024:1058	the obtained supramolecular polymer	1024:1058	The sophisticated nanomedicine constructed from the obtained supramolecular polymer can be specifically delivered to tumor sites and rapidly excreted from body after drug release, thus effectively avoiding systemic toxicity, especially long-term immunotoxicity.
29874067	0	52	theme	Long-Term	87:95	arg1	Immunotoxicity					97:110	Negligible Long-Term Immunotoxicity	76:110	Negligible Long-Term Immunotoxicity	76:110	Supramolecular Polymer-Based Nanomedicine: High Therapeutic Performance and Negligible Long-Term Immunotoxicity.
29874067	3	53	theme	supramolecular	553:566	arg1	polymerization					568:581	The supramolecular polymerization	549:581	The supramolecular polymerization	549:581	The supramolecular polymerization remarkably increases the solubility of CPT by a factor of 232 and effectively inhibits its lactone ring opening in physiological environment, which is favorable for intravenous formulation and maintenance of the therapeutic efficacy.
29874067	2	54	theme	disulfide	533:541	arg1	bond					543:546	a glutathione-cleavable disulfide bond	509:546	a glutathione-cleavable disulfide bond	509:546	Herein, we innovatively develop a theranostic supramolecular polymer using β-cyclodextrin as the host and camptothecin (CPT) as the guest linked by a glutathione-cleavable disulfide bond.
29874067	3	55	theme	intravenous	748:758	arg1	formulation					760:770	formulation	760:770	formulation	760:770	The supramolecular polymerization remarkably increases the solubility of CPT by a factor of 232 and effectively inhibits its lactone ring opening in physiological environment, which is favorable for intravenous formulation and maintenance of the therapeutic efficacy.
29874067	2	56	theme	theranostic	395:405	arg1	polymer					422:428	a theranostic supramolecular polymer	393:428	a theranostic supramolecular polymer using β-cyclodextrin as the host and camptothecin (CPT) as the guest linked by a glutathione-cleavable disulfide bond	393:546	Herein, we innovatively develop a theranostic supramolecular polymer using β-cyclodextrin as the host and camptothecin (CPT) as the guest linked by a glutathione-cleavable disulfide bond.
31641523	4	0	theme	Research	494:501	arg1	design					503:508	Research design	494:508	Research design	494:508	Research design and methods Zucker diabetic fatty rats (n=32) were randomly divided into four groups, and had respectively gastric administration of normal saline (control), metformin (215.15 mg/kg/day), acarbose (32.27 mg/kg/day), or sitagliptin (10.76 mg/kg/day) for 4 weeks.
31641523	10	1	theme	Lactobacillus	1453:1465	arg1	abundance					1434:1442	the relative abundance	1421:1442	the relative abundance of genus Lactobacillus	1421:1465	Metformin and sitagliptin increased the relative abundance of genus Lactobacillus.
31641523	3	2	from	comparisons	427:437	arg1	composition					481:491	gut microbiota composition	466:491	gut microbiota composition	466:491	The aim of the study was to observe the comparisons among those medications on gut microbiota composition.
31641523	6	3	theme	fecal	940:944	arg1	samples					946:952	the fecal samples	936:952	the fecal samples	936:952	16S rRNA gene sequencing was used to access the microbiota in the fecal samples.
31641523	12	4	theme	beneficial	1728:1737	arg1	bacteria					1739:1746	the beneficial bacteria	1724:1746	the beneficial bacteria	1724:1746	Conclusions Metformin, acarbose and sitagliptin exerted different effects on the composition of gut microbiota and selectively increased the beneficial bacteria.
31641523	0	5	theme	gut	63:65	arg1	microbiota					67:76	gut microbiota	63:76	gut microbiota in Zucker diabetic fatty rats	63:106	Effects of metformin, acarbose, and sitagliptin monotherapy on gut microbiota in Zucker diabetic fatty rats.
31641523	4	6	theme	methods	514:520	arg1	n=32					550:553	n=32	550:553	n=32	550:553	Research design and methods Zucker diabetic fatty rats (n=32) were randomly divided into four groups, and had respectively gastric administration of normal saline (control), metformin (215.15 mg/kg/day), acarbose (32.27 mg/kg/day), or sitagliptin (10.76 mg/kg/day) for 4 weeks.
31641523	4	6	theme	methods	514:520	arg1	rats					544:547	Research design and methods Zucker diabetic fatty rats	494:547	Research design and methods Zucker diabetic fatty rats (n=32)	494:554	Research design and methods Zucker diabetic fatty rats (n=32) were randomly divided into four groups, and had respectively gastric administration of normal saline (control), metformin (215.15 mg/kg/day), acarbose (32.27 mg/kg/day), or sitagliptin (10.76 mg/kg/day) for 4 weeks.
31641523	10	7	theme	genus	1447:1451	arg1	Lactobacillus					1453:1465	genus Lactobacillus	1447:1465	genus Lactobacillus	1447:1465	Metformin and sitagliptin increased the relative abundance of genus Lactobacillus.
31641523	11	8	theme	acarbose	1571:1578	arg1	group					1580:1584	acarbose group	1571:1584	acarbose group	1571:1584	Metagenomic prediction showed that the functional profiles of carbohydrate metabolism were enriched in acarbose group.
31641523	4	9	theme	metformin	668:676	arg1	administration					625:638	gastric administration	617:638	gastric administration of normal saline (control), metformin (215.15 mg/kg/day), acarbose (32.27 mg/kg/day), or sitagliptin (10.76 mg/kg/day) for 4 weeks	617:769	Research design and methods Zucker diabetic fatty rats (n=32) were randomly divided into four groups, and had respectively gastric administration of normal saline (control), metformin (215.15 mg/kg/day), acarbose (32.27 mg/kg/day), or sitagliptin (10.76 mg/kg/day) for 4 weeks.
31641523	0	10	theme	Zucker	81:86	arg1	rats					103:106	Zucker diabetic fatty rats	81:106	Zucker diabetic fatty rats	81:106	Effects of metformin, acarbose, and sitagliptin monotherapy on gut microbiota in Zucker diabetic fatty rats.
31641523	0	11	from	Effects	0:6	arg1	microbiota					67:76	gut microbiota	63:76	gut microbiota in Zucker diabetic fatty rats	63:106	Effects of metformin, acarbose, and sitagliptin monotherapy on gut microbiota in Zucker diabetic fatty rats.
31641523	4	12	theme	sitagliptin	729:739	arg1	administration					625:638	gastric administration	617:638	gastric administration of normal saline (control), metformin (215.15 mg/kg/day), acarbose (32.27 mg/kg/day), or sitagliptin (10.76 mg/kg/day) for 4 weeks	617:769	Research design and methods Zucker diabetic fatty rats (n=32) were randomly divided into four groups, and had respectively gastric administration of normal saline (control), metformin (215.15 mg/kg/day), acarbose (32.27 mg/kg/day), or sitagliptin (10.76 mg/kg/day) for 4 weeks.
31641523	4	13	theme	saline	650:655	arg1	administration					625:638	gastric administration	617:638	gastric administration of normal saline (control), metformin (215.15 mg/kg/day), acarbose (32.27 mg/kg/day), or sitagliptin (10.76 mg/kg/day) for 4 weeks	617:769	Research design and methods Zucker diabetic fatty rats (n=32) were randomly divided into four groups, and had respectively gastric administration of normal saline (control), metformin (215.15 mg/kg/day), acarbose (32.27 mg/kg/day), or sitagliptin (10.76 mg/kg/day) for 4 weeks.
31641523	9	14	theme	relative	1278:1285	arg1	higher					1359:1364	higher	1359:1364	higher	1359:1364	The relative abundance of genera Ruminococcus 2 and Bifidobacterium was dramatically higher in acarbose group.
31641523	9	14	theme	relative	1278:1285	arg1	abundance					1287:1295	The relative abundance	1274:1295	The relative abundance of genera Ruminococcus 2 and Bifidobacterium	1274:1340	The relative abundance of genera Ruminococcus 2 and Bifidobacterium was dramatically higher in acarbose group.
31641523	4	15	theme	acarbose	698:705	arg1	administration					625:638	gastric administration	617:638	gastric administration of normal saline (control), metformin (215.15 mg/kg/day), acarbose (32.27 mg/kg/day), or sitagliptin (10.76 mg/kg/day) for 4 weeks	617:769	Research design and methods Zucker diabetic fatty rats (n=32) were randomly divided into four groups, and had respectively gastric administration of normal saline (control), metformin (215.15 mg/kg/day), acarbose (32.27 mg/kg/day), or sitagliptin (10.76 mg/kg/day) for 4 weeks.
31641523	3	16	theme	microbiota	470:479	arg1	composition					481:491	gut microbiota composition	466:491	gut microbiota composition	466:491	The aim of the study was to observe the comparisons among those medications on gut microbiota composition.
31641523	0	17	theme	fatty	97:101	arg1	rats					103:106	Zucker diabetic fatty rats	81:106	Zucker diabetic fatty rats	81:106	Effects of metformin, acarbose, and sitagliptin monotherapy on gut microbiota in Zucker diabetic fatty rats.
31641523	1	18	theme	gut	157:159	arg1	microbiota					161:170	gut microbiota	157:170	gut microbiota	157:170	Objective Recent studies have demonstrated that gut microbiota was closely related to metabolic disorders such as type 2 diabetes.
31641523	0	19	theme	diabetic	88:95	arg1	rats					103:106	Zucker diabetic fatty rats	81:106	Zucker diabetic fatty rats	81:106	Effects of metformin, acarbose, and sitagliptin monotherapy on gut microbiota in Zucker diabetic fatty rats.
31641523	9	20	theme	genera	1300:1305	arg1	Ruminococcus					1307:1318	genera Ruminococcus 2	1300:1320	genera Ruminococcus 2	1300:1320	The relative abundance of genera Ruminococcus 2 and Bifidobacterium was dramatically higher in acarbose group.
31641523	7	21	theme	postprandial	1046:1057	arg1	levels					1073:1078	fasting and postprandial blood glucose levels	1034:1078	fasting and postprandial blood glucose levels	1034:1078	Results Metformin, acarbose, and sitagliptin monotherapy effectively decreased fasting and postprandial blood glucose levels (p<0.001).
31641523	4	22	theme	normal	643:648	arg1	saline					650:655	normal saline	643:655	normal saline (control)	643:665	Research design and methods Zucker diabetic fatty rats (n=32) were randomly divided into four groups, and had respectively gastric administration of normal saline (control), metformin (215.15 mg/kg/day), acarbose (32.27 mg/kg/day), or sitagliptin (10.76 mg/kg/day) for 4 weeks.
31641523	4	22	theme	normal	643:648	arg1	control					658:664	control	658:664	control	658:664	Research design and methods Zucker diabetic fatty rats (n=32) were randomly divided into four groups, and had respectively gastric administration of normal saline (control), metformin (215.15 mg/kg/day), acarbose (32.27 mg/kg/day), or sitagliptin (10.76 mg/kg/day) for 4 weeks.
31641523	1	23	theme	Recent	119:124	arg1	studies					126:132	Objective Recent studies	109:132	Objective Recent studies	109:132	Objective Recent studies have demonstrated that gut microbiota was closely related to metabolic disorders such as type 2 diabetes.
31641523	7	24	theme	blood	1059:1063	arg1	levels					1073:1078	fasting and postprandial blood glucose levels	1034:1078	fasting and postprandial blood glucose levels	1034:1078	Results Metformin, acarbose, and sitagliptin monotherapy effectively decreased fasting and postprandial blood glucose levels (p<0.001).
31641523	5	25	theme	tolerance	837:845	arg1	test					847:850	an intragastric starch tolerance test	814:850	an intragastric starch tolerance test after the treatments	814:871	Blood glucose levels were measured during an intragastric starch tolerance test after the treatments.
31641523	5	26	theme	Blood	772:776	arg1	levels					786:791	Blood glucose levels	772:791	Blood glucose levels	772:791	Blood glucose levels were measured during an intragastric starch tolerance test after the treatments.
31641523	7	27	theme	glucose	1065:1071	arg1	levels					1073:1078	fasting and postprandial blood glucose levels	1034:1078	fasting and postprandial blood glucose levels	1034:1078	Results Metformin, acarbose, and sitagliptin monotherapy effectively decreased fasting and postprandial blood glucose levels (p<0.001).
31641523	5	28	theme	intragastric	817:828	arg1	test					847:850	an intragastric starch tolerance test	814:850	an intragastric starch tolerance test after the treatments	814:871	Blood glucose levels were measured during an intragastric starch tolerance test after the treatments.
31641523	13	29	theme	drugs	1855:1859	arg1	effects					1827:1833	the hypoglycemic effects	1810:1833	the hypoglycemic effects of the antidiabetic drugs	1810:1859	Supplementation with specific probiotics may further improve the hypoglycemic effects of the antidiabetic drugs.
31641523	13	30	theme	specific	1770:1777	arg1	probiotics					1779:1788	specific probiotics	1770:1788	specific probiotics	1770:1788	Supplementation with specific probiotics may further improve the hypoglycemic effects of the antidiabetic drugs.
31641523	5	31	theme	glucose	778:784	arg1	levels					786:791	Blood glucose levels	772:791	Blood glucose levels	772:791	Blood glucose levels were measured during an intragastric starch tolerance test after the treatments.
31641523	12	32	theme	different	1643:1651	arg1	effects					1653:1659	different effects	1643:1659	different effects	1643:1659	Conclusions Metformin, acarbose and sitagliptin exerted different effects on the composition of gut microbiota and selectively increased the beneficial bacteria.
31641523	13	33	theme	antidiabetic	1842:1853	arg1	drugs					1855:1859	the antidiabetic drugs	1838:1859	the antidiabetic drugs	1838:1859	Supplementation with specific probiotics may further improve the hypoglycemic effects of the antidiabetic drugs.
31641523	9	34	theme	acarbose	1369:1376	arg1	group					1378:1382	acarbose group	1369:1382	acarbose group	1369:1382	The relative abundance of genera Ruminococcus 2 and Bifidobacterium was dramatically higher in acarbose group.
31641523	4	35	theme	gastric	617:623	arg1	administration					625:638	gastric administration	617:638	gastric administration of normal saline (control), metformin (215.15 mg/kg/day), acarbose (32.27 mg/kg/day), or sitagliptin (10.76 mg/kg/day) for 4 weeks	617:769	Research design and methods Zucker diabetic fatty rats (n=32) were randomly divided into four groups, and had respectively gastric administration of normal saline (control), metformin (215.15 mg/kg/day), acarbose (32.27 mg/kg/day), or sitagliptin (10.76 mg/kg/day) for 4 weeks.
31641523	0	36	theme	metformin	11:19	arg1	Effects					0:6	Effects	0:6	Effects of metformin, acarbose, and sitagliptin monotherapy on gut microbiota in Zucker diabetic fatty rats	0:106	Effects of metformin, acarbose, and sitagliptin monotherapy on gut microbiota in Zucker diabetic fatty rats.
31641523	9	37	theme	Bifidobacterium	1326:1340	arg1	higher					1359:1364	higher	1359:1364	higher	1359:1364	The relative abundance of genera Ruminococcus 2 and Bifidobacterium was dramatically higher in acarbose group.
31641523	9	37	theme	Bifidobacterium	1326:1340	arg1	abundance					1287:1295	The relative abundance	1274:1295	The relative abundance of genera Ruminococcus 2 and Bifidobacterium	1274:1340	The relative abundance of genera Ruminococcus 2 and Bifidobacterium was dramatically higher in acarbose group.
31641523	6	38	used	used	903:906	arg2	sequencing					888:897	16S rRNA gene sequencing	874:897	16S rRNA gene sequencing	874:897	16S rRNA gene sequencing was used to access the microbiota in the fecal samples.
31641523	0	39	from	microbiota	67:76	arg1	rats					103:106	Zucker diabetic fatty rats	81:106	Zucker diabetic fatty rats	81:106	Effects of metformin, acarbose, and sitagliptin monotherapy on gut microbiota in Zucker diabetic fatty rats.
31641523	11	40	theme	Metagenomic	1468:1478	arg1	prediction					1480:1489	Metagenomic prediction	1468:1489	Metagenomic prediction	1468:1489	Metagenomic prediction showed that the functional profiles of carbohydrate metabolism were enriched in acarbose group.
31641523	6	41	theme	gene	883:886	arg1	sequencing					888:897	16S rRNA gene sequencing	874:897	16S rRNA gene sequencing	874:897	16S rRNA gene sequencing was used to access the microbiota in the fecal samples.
31641523	3	42	theme	study	402:406	arg1	aim					391:393	The aim	387:393	The aim of the study	387:406	The aim of the study was to observe the comparisons among those medications on gut microbiota composition.
31641523	13	43	with	Supplementation	1749:1763	arg1	probiotics					1779:1788	specific probiotics	1770:1788	specific probiotics	1770:1788	Supplementation with specific probiotics may further improve the hypoglycemic effects of the antidiabetic drugs.
31641523	7	44	dep	Results	955:961	arg1	monotherapy					1000:1010	sitagliptin monotherapy	988:1010	sitagliptin monotherapy	988:1010	Results Metformin, acarbose, and sitagliptin monotherapy effectively decreased fasting and postprandial blood glucose levels (p<0.001).
31641523	7	44	dep	Results	955:961	arg1	Results					955:961	Results Metformin, acarbose, and sitagliptin monotherapy	955:1010	Results Metformin, acarbose, and sitagliptin monotherapy	955:1010	Results Metformin, acarbose, and sitagliptin monotherapy effectively decreased fasting and postprandial blood glucose levels (p<0.001).
31641523	7	44	dep	Results	955:961	arg1	acarbose					974:981	acarbose	974:981	acarbose	974:981	Results Metformin, acarbose, and sitagliptin monotherapy effectively decreased fasting and postprandial blood glucose levels (p<0.001).
31641523	7	44	dep	Results	955:961	arg1	Metformin					963:971	Metformin	963:971	Metformin	963:971	Results Metformin, acarbose, and sitagliptin monotherapy effectively decreased fasting and postprandial blood glucose levels (p<0.001).
31641523	1	45	theme	metabolic	195:203	arg1	diabetes					230:237	type 2 diabetes	223:237	type 2 diabetes	223:237	Objective Recent studies have demonstrated that gut microbiota was closely related to metabolic disorders such as type 2 diabetes.
31641523	1	45	theme	metabolic	195:203	arg1	disorders					205:213	metabolic disorders	195:213	metabolic disorders such as type 2 diabetes	195:237	Objective Recent studies have demonstrated that gut microbiota was closely related to metabolic disorders such as type 2 diabetes.
31641523	0	46	theme	acarbose	22:29	arg1	Effects					0:6	Effects	0:6	Effects of metformin, acarbose, and sitagliptin monotherapy on gut microbiota in Zucker diabetic fatty rats	0:106	Effects of metformin, acarbose, and sitagliptin monotherapy on gut microbiota in Zucker diabetic fatty rats.
31641523	4	47	theme	diabetic	529:536	arg1	n=32					550:553	n=32	550:553	n=32	550:553	Research design and methods Zucker diabetic fatty rats (n=32) were randomly divided into four groups, and had respectively gastric administration of normal saline (control), metformin (215.15 mg/kg/day), acarbose (32.27 mg/kg/day), or sitagliptin (10.76 mg/kg/day) for 4 weeks.
31641523	4	47	theme	diabetic	529:536	arg1	rats					544:547	Research design and methods Zucker diabetic fatty rats	494:547	Research design and methods Zucker diabetic fatty rats (n=32)	494:554	Research design and methods Zucker diabetic fatty rats (n=32) were randomly divided into four groups, and had respectively gastric administration of normal saline (control), metformin (215.15 mg/kg/day), acarbose (32.27 mg/kg/day), or sitagliptin (10.76 mg/kg/day) for 4 weeks.
31641523	7	48	theme	fasting	1034:1040	arg1	levels					1073:1078	fasting and postprandial blood glucose levels	1034:1078	fasting and postprandial blood glucose levels	1034:1078	Results Metformin, acarbose, and sitagliptin monotherapy effectively decreased fasting and postprandial blood glucose levels (p<0.001).
31641523	8	49	theme	specific	1116:1123	arg1	cluster					1125:1131	specific cluster	1116:1131	specific cluster	1116:1131	Acarbose group displayed specific cluster and enterotype mainly composed by Ruminococcus 2 while Lactobacillus was the dominant bacterium in the enterotype of the other three groups.
31641523	8	50	theme	other	1254:1258	arg1	groups					1266:1271	the other three groups	1250:1271	the other three groups	1250:1271	Acarbose group displayed specific cluster and enterotype mainly composed by Ruminococcus 2 while Lactobacillus was the dominant bacterium in the enterotype of the other three groups.
31641523	0	51	theme	sitagliptin	36:46	arg1	monotherapy					48:58	sitagliptin monotherapy	36:58	sitagliptin monotherapy	36:58	Effects of metformin, acarbose, and sitagliptin monotherapy on gut microbiota in Zucker diabetic fatty rats.
31641523	4	52	theme	Zucker	522:527	arg1	n=32					550:553	n=32	550:553	n=32	550:553	Research design and methods Zucker diabetic fatty rats (n=32) were randomly divided into four groups, and had respectively gastric administration of normal saline (control), metformin (215.15 mg/kg/day), acarbose (32.27 mg/kg/day), or sitagliptin (10.76 mg/kg/day) for 4 weeks.
31641523	4	52	theme	Zucker	522:527	arg1	rats					544:547	Research design and methods Zucker diabetic fatty rats	494:547	Research design and methods Zucker diabetic fatty rats (n=32)	494:554	Research design and methods Zucker diabetic fatty rats (n=32) were randomly divided into four groups, and had respectively gastric administration of normal saline (control), metformin (215.15 mg/kg/day), acarbose (32.27 mg/kg/day), or sitagliptin (10.76 mg/kg/day) for 4 weeks.
31641523	8	53	theme	Acarbose	1091:1098	arg1	group					1100:1104	Acarbose group	1091:1104	Acarbose group	1091:1104	Acarbose group displayed specific cluster and enterotype mainly composed by Ruminococcus 2 while Lactobacillus was the dominant bacterium in the enterotype of the other three groups.
31641523	6	54	theme	16S	874:876	arg1	sequencing					888:897	16S rRNA gene sequencing	874:897	16S rRNA gene sequencing	874:897	16S rRNA gene sequencing was used to access the microbiota in the fecal samples.
31641523	3	55	theme	gut	466:468	arg1	composition					481:491	gut microbiota composition	466:491	gut microbiota composition	466:491	The aim of the study was to observe the comparisons among those medications on gut microbiota composition.
31641523	6	56	theme	rRNA	878:881	arg1	sequencing					888:897	16S rRNA gene sequencing	874:897	16S rRNA gene sequencing	874:897	16S rRNA gene sequencing was used to access the microbiota in the fecal samples.
31641523	13	57	theme	hypoglycemic	1814:1825	arg1	effects					1827:1833	the hypoglycemic effects	1810:1833	the hypoglycemic effects of the antidiabetic drugs	1810:1859	Supplementation with specific probiotics may further improve the hypoglycemic effects of the antidiabetic drugs.
31641523	8	58	theme	groups	1266:1271	arg1	enterotype					1236:1245	the enterotype	1232:1245	the enterotype of the other three groups	1232:1271	Acarbose group displayed specific cluster and enterotype mainly composed by Ruminococcus 2 while Lactobacillus was the dominant bacterium in the enterotype of the other three groups.
31641523	11	59	theme	functional	1507:1516	arg1	profiles					1518:1525	the functional profiles	1503:1525	the functional profiles of carbohydrate metabolism	1503:1552	Metagenomic prediction showed that the functional profiles of carbohydrate metabolism were enriched in acarbose group.
31641523	4	60	theme	fatty	538:542	arg1	n=32					550:553	n=32	550:553	n=32	550:553	Research design and methods Zucker diabetic fatty rats (n=32) were randomly divided into four groups, and had respectively gastric administration of normal saline (control), metformin (215.15 mg/kg/day), acarbose (32.27 mg/kg/day), or sitagliptin (10.76 mg/kg/day) for 4 weeks.
31641523	4	60	theme	fatty	538:542	arg1	rats					544:547	Research design and methods Zucker diabetic fatty rats	494:547	Research design and methods Zucker diabetic fatty rats (n=32)	494:554	Research design and methods Zucker diabetic fatty rats (n=32) were randomly divided into four groups, and had respectively gastric administration of normal saline (control), metformin (215.15 mg/kg/day), acarbose (32.27 mg/kg/day), or sitagliptin (10.76 mg/kg/day) for 4 weeks.
31641523	6	61	from	access	911:916	arg1	samples					946:952	the fecal samples	936:952	the fecal samples	936:952	16S rRNA gene sequencing was used to access the microbiota in the fecal samples.
31641523	10	62	theme	relative	1425:1432	arg1	abundance					1434:1442	the relative abundance	1421:1442	the relative abundance of genus Lactobacillus	1421:1465	Metformin and sitagliptin increased the relative abundance of genus Lactobacillus.
31641523	2	63	theme	antidiabetic	245:256	arg1	metformin					280:288	metformin	280:288	metformin	280:288	Oral antidiabetic medications including metformin, acarbose and sitagliptin lowered blood glucose levels via acting on the gastrointestinal tract.
31641523	2	63	theme	antidiabetic	245:256	arg1	medications					258:268	Oral antidiabetic medications	240:268	Oral antidiabetic medications including metformin, acarbose and sitagliptin	240:314	Oral antidiabetic medications including metformin, acarbose and sitagliptin lowered blood glucose levels via acting on the gastrointestinal tract.
31641523	2	63	theme	antidiabetic	245:256	arg1	sitagliptin					304:314	sitagliptin	304:314	sitagliptin	304:314	Oral antidiabetic medications including metformin, acarbose and sitagliptin lowered blood glucose levels via acting on the gastrointestinal tract.
31641523	2	63	theme	antidiabetic	245:256	arg1	acarbose					291:298	acarbose	291:298	acarbose	291:298	Oral antidiabetic medications including metformin, acarbose and sitagliptin lowered blood glucose levels via acting on the gastrointestinal tract.
31641523	8	64	from	bacterium	1219:1227	arg1	enterotype					1236:1245	the enterotype	1232:1245	the enterotype of the other three groups	1232:1271	Acarbose group displayed specific cluster and enterotype mainly composed by Ruminococcus 2 while Lactobacillus was the dominant bacterium in the enterotype of the other three groups.
31641523	1	65	theme	Objective	109:117	arg1	studies					126:132	Objective Recent studies	109:132	Objective Recent studies	109:132	Objective Recent studies have demonstrated that gut microbiota was closely related to metabolic disorders such as type 2 diabetes.
31641523	7	66	theme	sitagliptin	988:998	arg1	monotherapy					1000:1010	sitagliptin monotherapy	988:1010	sitagliptin monotherapy	988:1010	Results Metformin, acarbose, and sitagliptin monotherapy effectively decreased fasting and postprandial blood glucose levels (p<0.001).
31641523	7	66	theme	sitagliptin	988:998	arg1	Results					955:961	Results Metformin, acarbose, and sitagliptin monotherapy	955:1010	Results Metformin, acarbose, and sitagliptin monotherapy	955:1010	Results Metformin, acarbose, and sitagliptin monotherapy effectively decreased fasting and postprandial blood glucose levels (p<0.001).
31641523	12	67	dep	Conclusions	1587:1597	arg1	sitagliptin					1623:1633	sitagliptin	1623:1633	sitagliptin	1623:1633	Conclusions Metformin, acarbose and sitagliptin exerted different effects on the composition of gut microbiota and selectively increased the beneficial bacteria.
31641523	12	67	dep	Conclusions	1587:1597	arg1	Conclusions					1587:1597	Conclusions Metformin, acarbose and sitagliptin	1587:1633	Conclusions Metformin, acarbose and sitagliptin	1587:1633	Conclusions Metformin, acarbose and sitagliptin exerted different effects on the composition of gut microbiota and selectively increased the beneficial bacteria.
31641523	12	67	dep	Conclusions	1587:1597	arg1	acarbose					1610:1617	acarbose	1610:1617	acarbose	1610:1617	Conclusions Metformin, acarbose and sitagliptin exerted different effects on the composition of gut microbiota and selectively increased the beneficial bacteria.
31641523	12	67	dep	Conclusions	1587:1597	arg1	Metformin					1599:1607	Metformin	1599:1607	Metformin	1599:1607	Conclusions Metformin, acarbose and sitagliptin exerted different effects on the composition of gut microbiota and selectively increased the beneficial bacteria.
31641523	2	68	theme	Oral	240:243	arg1	metformin					280:288	metformin	280:288	metformin	280:288	Oral antidiabetic medications including metformin, acarbose and sitagliptin lowered blood glucose levels via acting on the gastrointestinal tract.
31641523	2	68	theme	Oral	240:243	arg1	medications					258:268	Oral antidiabetic medications	240:268	Oral antidiabetic medications including metformin, acarbose and sitagliptin	240:314	Oral antidiabetic medications including metformin, acarbose and sitagliptin lowered blood glucose levels via acting on the gastrointestinal tract.
31641523	2	68	theme	Oral	240:243	arg1	sitagliptin					304:314	sitagliptin	304:314	sitagliptin	304:314	Oral antidiabetic medications including metformin, acarbose and sitagliptin lowered blood glucose levels via acting on the gastrointestinal tract.
31641523	2	68	theme	Oral	240:243	arg1	acarbose					291:298	acarbose	291:298	acarbose	291:298	Oral antidiabetic medications including metformin, acarbose and sitagliptin lowered blood glucose levels via acting on the gastrointestinal tract.
31641523	12	69	theme	gut	1683:1685	arg1	microbiota					1687:1696	gut microbiota	1683:1696	gut microbiota	1683:1696	Conclusions Metformin, acarbose and sitagliptin exerted different effects on the composition of gut microbiota and selectively increased the beneficial bacteria.
31641523	2	70	theme	blood	324:328	arg1	levels					338:343	blood glucose levels	324:343	blood glucose levels	324:343	Oral antidiabetic medications including metformin, acarbose and sitagliptin lowered blood glucose levels via acting on the gastrointestinal tract.
31641523	8	71	theme	dominant	1210:1217	arg1	bacterium					1219:1227	the dominant bacterium	1206:1227	the dominant bacterium in the enterotype of the other three groups	1206:1271	Acarbose group displayed specific cluster and enterotype mainly composed by Ruminococcus 2 while Lactobacillus was the dominant bacterium in the enterotype of the other three groups.
31641523	8	71	theme	dominant	1210:1217	arg1	Lactobacillus					1188:1200	Lactobacillus	1188:1200	Lactobacillus	1188:1200	Acarbose group displayed specific cluster and enterotype mainly composed by Ruminococcus 2 while Lactobacillus was the dominant bacterium in the enterotype of the other three groups.
31641523	2	72	theme	glucose	330:336	arg1	levels					338:343	blood glucose levels	324:343	blood glucose levels	324:343	Oral antidiabetic medications including metformin, acarbose and sitagliptin lowered blood glucose levels via acting on the gastrointestinal tract.
31641523	7	73	dep	decreased	1024:1032	arg1	p<0.001					1081:1087	p<0.001	1081:1087	p<0.001	1081:1087	Results Metformin, acarbose, and sitagliptin monotherapy effectively decreased fasting and postprandial blood glucose levels (p<0.001).
31641523	11	74	theme	carbohydrate	1530:1541	arg1	metabolism					1543:1552	carbohydrate metabolism	1530:1552	carbohydrate metabolism	1530:1552	Metagenomic prediction showed that the functional profiles of carbohydrate metabolism were enriched in acarbose group.
31641523	2	75	theme	gastrointestinal	363:378	arg1	tract					380:384	the gastrointestinal tract	359:384	the gastrointestinal tract	359:384	Oral antidiabetic medications including metformin, acarbose and sitagliptin lowered blood glucose levels via acting on the gastrointestinal tract.
31641523	5	76	theme	starch	830:835	arg1	test					847:850	an intragastric starch tolerance test	814:850	an intragastric starch tolerance test after the treatments	814:871	Blood glucose levels were measured during an intragastric starch tolerance test after the treatments.
31641523	1	77	theme	type	223:226	arg1	diabetes					230:237	type 2 diabetes	223:237	type 2 diabetes	223:237	Objective Recent studies have demonstrated that gut microbiota was closely related to metabolic disorders such as type 2 diabetes.
31641523	0	78	theme	monotherapy	48:58	arg1	Effects					0:6	Effects	0:6	Effects of metformin, acarbose, and sitagliptin monotherapy on gut microbiota in Zucker diabetic fatty rats	0:106	Effects of metformin, acarbose, and sitagliptin monotherapy on gut microbiota in Zucker diabetic fatty rats.
31641523	4	79	theme	design	503:508	arg1	n=32					550:553	n=32	550:553	n=32	550:553	Research design and methods Zucker diabetic fatty rats (n=32) were randomly divided into four groups, and had respectively gastric administration of normal saline (control), metformin (215.15 mg/kg/day), acarbose (32.27 mg/kg/day), or sitagliptin (10.76 mg/kg/day) for 4 weeks.
31641523	4	79	theme	design	503:508	arg1	rats					544:547	Research design and methods Zucker diabetic fatty rats	494:547	Research design and methods Zucker diabetic fatty rats (n=32)	494:554	Research design and methods Zucker diabetic fatty rats (n=32) were randomly divided into four groups, and had respectively gastric administration of normal saline (control), metformin (215.15 mg/kg/day), acarbose (32.27 mg/kg/day), or sitagliptin (10.76 mg/kg/day) for 4 weeks.
31641523	12	80	theme	microbiota	1687:1696	arg1	composition					1668:1678	the composition	1664:1678	the composition of gut microbiota	1664:1696	Conclusions Metformin, acarbose and sitagliptin exerted different effects on the composition of gut microbiota and selectively increased the beneficial bacteria.
31641523	9	81	theme	Ruminococcus	1307:1318	arg1	higher					1359:1364	higher	1359:1364	higher	1359:1364	The relative abundance of genera Ruminococcus 2 and Bifidobacterium was dramatically higher in acarbose group.
31641523	9	81	theme	Ruminococcus	1307:1318	arg1	abundance					1287:1295	The relative abundance	1274:1295	The relative abundance of genera Ruminococcus 2 and Bifidobacterium	1274:1340	The relative abundance of genera Ruminococcus 2 and Bifidobacterium was dramatically higher in acarbose group.
31641523	11	82	theme	metabolism	1543:1552	arg1	profiles					1518:1525	the functional profiles	1503:1525	the functional profiles of carbohydrate metabolism	1503:1552	Metagenomic prediction showed that the functional profiles of carbohydrate metabolism were enriched in acarbose group.
31483241	12	0	theme	novel	1160:1164	arg1	species					1166:1172	a novel species	1158:1172	a novel species	1158:1172	Based on phenotypic and chemotaxonomic characteristics, strain T3246-1T was considered to represent a novel species of the genus Haloactinobacterium, for which the name Haloactinobacterium glacieicola sp.
31483241	7	1	theme	whole-cell	549:558	arg1	sugars					560:565	The whole-cell sugars	545:565	The whole-cell sugars	545:565	The whole-cell sugars were rhamnose, xylose and mannose.
31483241	7	1	theme	whole-cell	549:558	arg1	rhamnose					572:579	rhamnose	572:579	rhamnose	572:579	The whole-cell sugars were rhamnose, xylose and mannose.
31483241	12	2	theme	genus	1181:1185	arg1	Haloactinobacterium					1187:1205	the genus Haloactinobacterium	1177:1205	the genus Haloactinobacterium	1177:1205	Based on phenotypic and chemotaxonomic characteristics, strain T3246-1T was considered to represent a novel species of the genus Haloactinobacterium, for which the name Haloactinobacterium glacieicola sp.
31483241	2	3	theme	motile	107:112	arg1	bacterium					114:122	A Gram-stain-positive, rod-shaped and motile bacterium	69:122	A Gram-stain-positive, rod-shaped and motile bacterium	69:122	A Gram-stain-positive, rod-shaped and motile bacterium with lateral flagellum, designated T3246-1T, was isolated from an ice core, which was drilled from Hariqin Glacier on the Tibetan Plateau, PR China.
31483241	10	4	theme	YIM	906:908	arg1	93306T					910:915	Haloactinobacterium album YIM 93306T	880:915	Haloactinobacterium album YIM 93306T with 95.99 % similarity	880:939	Results of phylogenetic analysis based on 16S rRNA gene sequences showed that strain T3246-1T formed a lineage within the genus Haloactinobacterium and was closely related to Haloactinobacterium album YIM 93306T with 95.99 % similarity.
31483241	10	5	theme	Haloactinobacterium	880:898	arg1	93306T					910:915	Haloactinobacterium album YIM 93306T	880:915	Haloactinobacterium album YIM 93306T with 95.99 % similarity	880:939	Results of phylogenetic analysis based on 16S rRNA gene sequences showed that strain T3246-1T formed a lineage within the genus Haloactinobacterium and was closely related to Haloactinobacterium album YIM 93306T with 95.99 % similarity.
31483241	4	6	theme	T3246-1T	378:385	arg1	 0					404:405	 0	404:405	 0	404:405	The major fatty acid of strain T3246-1T was anteiso-C15 : 0.
31483241	4	6	theme	T3246-1T	378:385	arg1	acid					363:366	The major fatty acid	347:366	The major fatty acid of strain T3246-1T	347:385	The major fatty acid of strain T3246-1T was anteiso-C15 : 0.
31483241	1	7	theme	ice	59:61	arg1	core					63:66	an ice core	56:66	an ice core	56:66	nov., isolated from an ice core.
31483241	14	8	theme	1.13535T=JCM	1317:1328	arg1	32923T					1330:1335	=CGMCC 1.13535T=JCM 32923T	1310:1335	=CGMCC 1.13535T=JCM 32923T	1310:1335	The type strain is T3246-1T (=CGMCC 1.13535T=JCM 32923T).
31483241	14	8	theme	1.13535T=JCM	1317:1328	arg1	T3246-1T					1300:1307	T3246-1T	1300:1307	T3246-1T (=CGMCC 1.13535T=JCM 32923T)	1300:1336	The type strain is T3246-1T (=CGMCC 1.13535T=JCM 32923T).
31483241	4	9	theme	strain	371:376	arg1	T3246-1T					378:385	strain T3246-1T	371:385	strain T3246-1T	371:385	The major fatty acid of strain T3246-1T was anteiso-C15 : 0.
31483241	2	10	theme	Tibetan	246:252	arg1	Plateau					254:260	the Tibetan Plateau	242:260	the Tibetan Plateau	242:260	A Gram-stain-positive, rod-shaped and motile bacterium with lateral flagellum, designated T3246-1T, was isolated from an ice core, which was drilled from Hariqin Glacier on the Tibetan Plateau, PR China.
31483241	2	10	theme	Tibetan	246:252	arg1	China					266:270	PR China	263:270	PR China	263:270	A Gram-stain-positive, rod-shaped and motile bacterium with lateral flagellum, designated T3246-1T, was isolated from an ice core, which was drilled from Hariqin Glacier on the Tibetan Plateau, PR China.
31483241	10	11	theme	16S	747:749	arg1	sequences					761:769	16S rRNA gene sequences	747:769	16S rRNA gene sequences	747:769	Results of phylogenetic analysis based on 16S rRNA gene sequences showed that strain T3246-1T formed a lineage within the genus Haloactinobacterium and was closely related to Haloactinobacterium album YIM 93306T with 95.99 % similarity.
31483241	14	12	theme	=CGMCC	1310:1315	arg1	32923T					1330:1335	=CGMCC 1.13535T=JCM 32923T	1310:1335	=CGMCC 1.13535T=JCM 32923T	1310:1335	The type strain is T3246-1T (=CGMCC 1.13535T=JCM 32923T).
31483241	14	12	theme	=CGMCC	1310:1315	arg1	T3246-1T					1300:1307	T3246-1T	1300:1307	T3246-1T (=CGMCC 1.13535T=JCM 32923T)	1300:1336	The type strain is T3246-1T (=CGMCC 1.13535T=JCM 32923T).
31483241	8	13	theme	cell-wall	612:620	arg1	peptidoglycan					622:634	The major cell-wall peptidoglycan	602:634	The major cell-wall peptidoglycan	602:634	The major cell-wall peptidoglycan was lysine.
31483241	8	13	theme	cell-wall	612:620	arg1	lysine					640:645	lysine	640:645	lysine	640:645	The major cell-wall peptidoglycan was lysine.
31483241	3	14	dep	%	333:333	arg1	w/v					336:338	w/v	336:338	w/v	336:338	It grew optimally at 20 °C, pH 7-8 and in the presence of 3 % (w/v) NaCl.
31483241	9	15	theme	DNA	660:662	arg1	content					668:674	The genomic DNA G+C content	648:674	The genomic DNA G+C content of the strain	648:688	The genomic DNA G+C content of the strain was 71.4 mol%.
31483241	9	15	theme	DNA	660:662	arg1	%					702:702	71.4 mol%	694:702	71.4 mol%	694:702	The genomic DNA G+C content of the strain was 71.4 mol%.
31483241	12	16	theme	Haloactinobacterium	1187:1205	arg1	species					1166:1172	a novel species	1158:1172	a novel species	1158:1172	Based on phenotypic and chemotaxonomic characteristics, strain T3246-1T was considered to represent a novel species of the genus Haloactinobacterium, for which the name Haloactinobacterium glacieicola sp.
31483241	10	17	theme	analysis	729:736	arg1	Results					705:711	Results	705:711	Results of phylogenetic analysis based on 16S rRNA gene sequences	705:769	Results of phylogenetic analysis based on 16S rRNA gene sequences showed that strain T3246-1T formed a lineage within the genus Haloactinobacterium and was closely related to Haloactinobacterium album YIM 93306T with 95.99 % similarity.
31483241	0	18	theme	glacieicola	20:30	arg1	sp					32:33	Haloactinobacterium glacieicola sp	0:33	Haloactinobacterium glacieicola sp.	0:34	Haloactinobacterium glacieicola sp.
31483241	2	19	theme	lateral	129:135	arg1	flagellum					137:145	lateral flagellum	129:145	lateral flagellum	129:145	A Gram-stain-positive, rod-shaped and motile bacterium with lateral flagellum, designated T3246-1T, was isolated from an ice core, which was drilled from Hariqin Glacier on the Tibetan Plateau, PR China.
31483241	9	20	theme	G+C	664:666	arg1	content					668:674	The genomic DNA G+C content	648:674	The genomic DNA G+C content of the strain	648:688	The genomic DNA G+C content of the strain was 71.4 mol%.
31483241	9	20	theme	G+C	664:666	arg1	%					702:702	71.4 mol%	694:702	71.4 mol%	694:702	The genomic DNA G+C content of the strain was 71.4 mol%.
31483241	5	21	theme	Major	408:412	arg1	lipids					420:425	Major polar lipids	408:425	Major polar lipids	408:425	Major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol.
31483241	0	22	theme	Haloactinobacterium	0:18	arg1	sp					32:33	Haloactinobacterium glacieicola sp	0:33	Haloactinobacterium glacieicola sp.	0:34	Haloactinobacterium glacieicola sp.
31483241	3	23	theme	NaCl	341:344	arg1	presence					319:326	the presence	315:326	the presence of 3 % (w/v) NaCl	315:344	It grew optimally at 20 °C, pH 7-8 and in the presence of 3 % (w/v) NaCl.
31483241	6	24	theme	isoprenoid	525:534	arg1	quinone					536:542	the dominant isoprenoid quinone	512:542	the dominant isoprenoid quinone	512:542	MK-8 was the dominant isoprenoid quinone.
31483241	6	24	theme	isoprenoid	525:534	arg1	MK-8					503:506	MK-8	503:506	MK-8	503:506	MK-8 was the dominant isoprenoid quinone.
31483241	12	25	theme	name	1222:1225	arg1	sp					1259:1260	the name Haloactinobacterium glacieicola sp	1218:1260	the name Haloactinobacterium glacieicola sp	1218:1260	Based on phenotypic and chemotaxonomic characteristics, strain T3246-1T was considered to represent a novel species of the genus Haloactinobacterium, for which the name Haloactinobacterium glacieicola sp.
31483241	6	26	theme	dominant	516:523	arg1	quinone					536:542	the dominant isoprenoid quinone	512:542	the dominant isoprenoid quinone	512:542	MK-8 was the dominant isoprenoid quinone.
31483241	6	26	theme	dominant	516:523	arg1	MK-8					503:506	MK-8	503:506	MK-8	503:506	MK-8 was the dominant isoprenoid quinone.
31483241	9	27	theme	genomic	652:658	arg1	content					668:674	The genomic DNA G+C content	648:674	The genomic DNA G+C content of the strain	648:688	The genomic DNA G+C content of the strain was 71.4 mol%.
31483241	9	27	theme	genomic	652:658	arg1	%					702:702	71.4 mol%	694:702	71.4 mol%	694:702	The genomic DNA G+C content of the strain was 71.4 mol%.
31483241	4	28	theme	fatty	357:361	arg1	 0					404:405	 0	404:405	 0	404:405	The major fatty acid of strain T3246-1T was anteiso-C15 : 0.
31483241	4	28	theme	fatty	357:361	arg1	acid					363:366	The major fatty acid	347:366	The major fatty acid of strain T3246-1T	347:385	The major fatty acid of strain T3246-1T was anteiso-C15 : 0.
31483241	2	29	theme	PR	263:264	arg1	Plateau					254:260	the Tibetan Plateau	242:260	the Tibetan Plateau	242:260	A Gram-stain-positive, rod-shaped and motile bacterium with lateral flagellum, designated T3246-1T, was isolated from an ice core, which was drilled from Hariqin Glacier on the Tibetan Plateau, PR China.
31483241	2	29	theme	PR	263:264	arg1	China					266:270	PR China	263:270	PR China	263:270	A Gram-stain-positive, rod-shaped and motile bacterium with lateral flagellum, designated T3246-1T, was isolated from an ice core, which was drilled from Hariqin Glacier on the Tibetan Plateau, PR China.
31483241	2	30	attach	isolated	173:180	arg1	core					194:197	an ice core	187:197	an ice core	187:197	A Gram-stain-positive, rod-shaped and motile bacterium with lateral flagellum, designated T3246-1T, was isolated from an ice core, which was drilled from Hariqin Glacier on the Tibetan Plateau, PR China.
31483241	2	30	attach	isolated	173:180	arg2	bacterium					114:122	A Gram-stain-positive, rod-shaped and motile bacterium	69:122	A Gram-stain-positive, rod-shaped and motile bacterium	69:122	A Gram-stain-positive, rod-shaped and motile bacterium with lateral flagellum, designated T3246-1T, was isolated from an ice core, which was drilled from Hariqin Glacier on the Tibetan Plateau, PR China.
31483241	10	31	theme	phylogenetic	716:727	arg1	analysis					729:736	phylogenetic analysis	716:736	phylogenetic analysis based on 16S rRNA gene sequences	716:769	Results of phylogenetic analysis based on 16S rRNA gene sequences showed that strain T3246-1T formed a lineage within the genus Haloactinobacterium and was closely related to Haloactinobacterium album YIM 93306T with 95.99 % similarity.
31483241	10	32	with	93306T	910:915	arg1	similarity					930:939	95.99 % similarity	922:939	95.99 % similarity	922:939	Results of phylogenetic analysis based on 16S rRNA gene sequences showed that strain T3246-1T formed a lineage within the genus Haloactinobacterium and was closely related to Haloactinobacterium album YIM 93306T with 95.99 % similarity.
31483241	4	33	theme	major	351:355	arg1	 0					404:405	 0	404:405	 0	404:405	The major fatty acid of strain T3246-1T was anteiso-C15 : 0.
31483241	4	33	theme	major	351:355	arg1	acid					363:366	The major fatty acid	347:366	The major fatty acid of strain T3246-1T	347:385	The major fatty acid of strain T3246-1T was anteiso-C15 : 0.
31483241	9	34	theme	strain	683:688	arg1	content					668:674	The genomic DNA G+C content	648:674	The genomic DNA G+C content of the strain	648:688	The genomic DNA G+C content of the strain was 71.4 mol%.
31483241	9	34	theme	strain	683:688	arg1	%					702:702	71.4 mol%	694:702	71.4 mol%	694:702	The genomic DNA G+C content of the strain was 71.4 mol%.
31483241	2	35	with	bacterium	114:122	arg1	flagellum					137:145	lateral flagellum	129:145	lateral flagellum	129:145	A Gram-stain-positive, rod-shaped and motile bacterium with lateral flagellum, designated T3246-1T, was isolated from an ice core, which was drilled from Hariqin Glacier on the Tibetan Plateau, PR China.
31483241	10	36	theme	gene	756:759	arg1	sequences					761:769	16S rRNA gene sequences	747:769	16S rRNA gene sequences	747:769	Results of phylogenetic analysis based on 16S rRNA gene sequences showed that strain T3246-1T formed a lineage within the genus Haloactinobacterium and was closely related to Haloactinobacterium album YIM 93306T with 95.99 % similarity.
31483241	11	37	theme	album	1028:1032	arg1	93306T					1038:1043	Haloactinobacterium album YIM 93306T	1008:1043	Haloactinobacterium album YIM 93306T	1008:1043	The average nucleotide identity value between strain T3246-1T and Haloactinobacterium album YIM 93306T was 76.65 %.
31483241	2	38	theme	rod-shaped	92:101	arg1	bacterium					114:122	A Gram-stain-positive, rod-shaped and motile bacterium	69:122	A Gram-stain-positive, rod-shaped and motile bacterium	69:122	A Gram-stain-positive, rod-shaped and motile bacterium with lateral flagellum, designated T3246-1T, was isolated from an ice core, which was drilled from Hariqin Glacier on the Tibetan Plateau, PR China.
31483241	12	39	dep	Haloactinobacterium	1227:1245	arg1	glacieicola					1247:1257	glacieicola	1247:1257	glacieicola	1247:1257	Based on phenotypic and chemotaxonomic characteristics, strain T3246-1T was considered to represent a novel species of the genus Haloactinobacterium, for which the name Haloactinobacterium glacieicola sp.
31483241	10	40	theme	rRNA	751:754	arg1	sequences					761:769	16S rRNA gene sequences	747:769	16S rRNA gene sequences	747:769	Results of phylogenetic analysis based on 16S rRNA gene sequences showed that strain T3246-1T formed a lineage within the genus Haloactinobacterium and was closely related to Haloactinobacterium album YIM 93306T with 95.99 % similarity.
31483241	3	41	theme	3 	331:332	arg1	%					333:333	%	333:333	%	333:333	It grew optimally at 20 °C, pH 7-8 and in the presence of 3 % (w/v) NaCl.
31483241	8	42	theme	major	606:610	arg1	peptidoglycan					622:634	The major cell-wall peptidoglycan	602:634	The major cell-wall peptidoglycan	602:634	The major cell-wall peptidoglycan was lysine.
31483241	8	42	theme	major	606:610	arg1	lysine					640:645	lysine	640:645	lysine	640:645	The major cell-wall peptidoglycan was lysine.
31483241	9	43	theme	71.4 mol	694:701	arg1	content					668:674	The genomic DNA G+C content	648:674	The genomic DNA G+C content of the strain	648:688	The genomic DNA G+C content of the strain was 71.4 mol%.
31483241	9	43	theme	71.4 mol	694:701	arg1	%					702:702	71.4 mol%	694:702	71.4 mol%	694:702	The genomic DNA G+C content of the strain was 71.4 mol%.
31483241	11	44	theme	Haloactinobacterium	1008:1026	arg1	93306T					1038:1043	Haloactinobacterium album YIM 93306T	1008:1043	Haloactinobacterium album YIM 93306T	1008:1043	The average nucleotide identity value between strain T3246-1T and Haloactinobacterium album YIM 93306T was 76.65 %.
31483241	11	45	theme	nucleotide	954:963	arg1	value					974:978	The average nucleotide identity value	942:978	The average nucleotide identity value between strain T3246-1T and Haloactinobacterium album YIM 93306T	942:1043	The average nucleotide identity value between strain T3246-1T and Haloactinobacterium album YIM 93306T was 76.65 %.
31483241	11	45	theme	nucleotide	954:963	arg1	%					1055:1055	76.65 %	1049:1055	76.65 %	1049:1055	The average nucleotide identity value between strain T3246-1T and Haloactinobacterium album YIM 93306T was 76.65 %.
31483241	3	46	theme	%	333:333	arg1	NaCl					341:344	3 % (w/v) NaCl	331:344	3 % (w/v) NaCl	331:344	It grew optimally at 20 °C, pH 7-8 and in the presence of 3 % (w/v) NaCl.
31483241	2	47	theme	Gram-stain-positive	71:89	arg1	bacterium					114:122	A Gram-stain-positive, rod-shaped and motile bacterium	69:122	A Gram-stain-positive, rod-shaped and motile bacterium	69:122	A Gram-stain-positive, rod-shaped and motile bacterium with lateral flagellum, designated T3246-1T, was isolated from an ice core, which was drilled from Hariqin Glacier on the Tibetan Plateau, PR China.
31483241	10	48	theme	album	900:904	arg1	93306T					910:915	Haloactinobacterium album YIM 93306T	880:915	Haloactinobacterium album YIM 93306T with 95.99 % similarity	880:939	Results of phylogenetic analysis based on 16S rRNA gene sequences showed that strain T3246-1T formed a lineage within the genus Haloactinobacterium and was closely related to Haloactinobacterium album YIM 93306T with 95.99 % similarity.
31483241	11	49	theme	identity	965:972	arg1	value					974:978	The average nucleotide identity value	942:978	The average nucleotide identity value between strain T3246-1T and Haloactinobacterium album YIM 93306T	942:1043	The average nucleotide identity value between strain T3246-1T and Haloactinobacterium album YIM 93306T was 76.65 %.
31483241	11	49	theme	identity	965:972	arg1	%					1055:1055	76.65 %	1049:1055	76.65 %	1049:1055	The average nucleotide identity value between strain T3246-1T and Haloactinobacterium album YIM 93306T was 76.65 %.
31483241	2	50	theme	ice	190:192	arg1	core					194:197	an ice core	187:197	an ice core	187:197	A Gram-stain-positive, rod-shaped and motile bacterium with lateral flagellum, designated T3246-1T, was isolated from an ice core, which was drilled from Hariqin Glacier on the Tibetan Plateau, PR China.
31483241	10	51	theme	strain	783:788	arg1	T3246-1T					790:797	strain T3246-1T	783:797	strain T3246-1T	783:797	Results of phylogenetic analysis based on 16S rRNA gene sequences showed that strain T3246-1T formed a lineage within the genus Haloactinobacterium and was closely related to Haloactinobacterium album YIM 93306T with 95.99 % similarity.
31483241	10	52	theme	genus	827:831	arg1	Haloactinobacterium					833:851	the genus Haloactinobacterium	823:851	the genus Haloactinobacterium	823:851	Results of phylogenetic analysis based on 16S rRNA gene sequences showed that strain T3246-1T formed a lineage within the genus Haloactinobacterium and was closely related to Haloactinobacterium album YIM 93306T with 95.99 % similarity.
31483241	5	53	theme	polar	414:418	arg1	lipids					420:425	Major polar lipids	408:425	Major polar lipids	408:425	Major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol.
31483241	11	54	theme	average	946:952	arg1	value					974:978	The average nucleotide identity value	942:978	The average nucleotide identity value between strain T3246-1T and Haloactinobacterium album YIM 93306T	942:1043	The average nucleotide identity value between strain T3246-1T and Haloactinobacterium album YIM 93306T was 76.65 %.
31483241	11	54	theme	average	946:952	arg1	%					1055:1055	76.65 %	1049:1055	76.65 %	1049:1055	The average nucleotide identity value between strain T3246-1T and Haloactinobacterium album YIM 93306T was 76.65 %.
31483241	12	55	theme	Haloactinobacterium	1227:1245	arg1	sp					1259:1260	the name Haloactinobacterium glacieicola sp	1218:1260	the name Haloactinobacterium glacieicola sp	1218:1260	Based on phenotypic and chemotaxonomic characteristics, strain T3246-1T was considered to represent a novel species of the genus Haloactinobacterium, for which the name Haloactinobacterium glacieicola sp.
31483241	10	56	theme	%	928:928	arg1	similarity					930:939	95.99 % similarity	922:939	95.99 % similarity	922:939	Results of phylogenetic analysis based on 16S rRNA gene sequences showed that strain T3246-1T formed a lineage within the genus Haloactinobacterium and was closely related to Haloactinobacterium album YIM 93306T with 95.99 % similarity.
31483241	11	57	theme	strain	988:993	arg1	T3246-1T					995:1002	strain T3246-1T	988:1002	strain T3246-1T	988:1002	The average nucleotide identity value between strain T3246-1T and Haloactinobacterium album YIM 93306T was 76.65 %.
31483241	11	58	theme	YIM	1034:1036	arg1	93306T					1038:1043	Haloactinobacterium album YIM 93306T	1008:1043	Haloactinobacterium album YIM 93306T	1008:1043	The average nucleotide identity value between strain T3246-1T and Haloactinobacterium album YIM 93306T was 76.65 %.
31483241	14	59	theme	type	1285:1288	arg1	T3246-1T					1300:1307	T3246-1T	1300:1307	T3246-1T (=CGMCC 1.13535T=JCM 32923T)	1300:1336	The type strain is T3246-1T (=CGMCC 1.13535T=JCM 32923T).
31483241	14	59	theme	type	1285:1288	arg1	strain					1290:1295	The type strain	1281:1295	The type strain	1281:1295	The type strain is T3246-1T (=CGMCC 1.13535T=JCM 32923T).
31483241	12	60	theme	phenotypic	1067:1076	arg1	characteristics					1097:1111	phenotypic and chemotaxonomic characteristics	1067:1111	phenotypic and chemotaxonomic characteristics	1067:1111	Based on phenotypic and chemotaxonomic characteristics, strain T3246-1T was considered to represent a novel species of the genus Haloactinobacterium, for which the name Haloactinobacterium glacieicola sp.
31483241	10	61	theme	95.99 	922:927	arg1	%					928:928	%	928:928	%	928:928	Results of phylogenetic analysis based on 16S rRNA gene sequences showed that strain T3246-1T formed a lineage within the genus Haloactinobacterium and was closely related to Haloactinobacterium album YIM 93306T with 95.99 % similarity.
31483241	12	62	theme	chemotaxonomic	1082:1095	arg1	characteristics					1097:1111	phenotypic and chemotaxonomic characteristics	1067:1111	phenotypic and chemotaxonomic characteristics	1067:1111	Based on phenotypic and chemotaxonomic characteristics, strain T3246-1T was considered to represent a novel species of the genus Haloactinobacterium, for which the name Haloactinobacterium glacieicola sp.
31483241	12	63	theme	strain	1114:1119	arg1	T3246-1T					1121:1128	strain T3246-1T	1114:1128	strain T3246-1T	1114:1128	Based on phenotypic and chemotaxonomic characteristics, strain T3246-1T was considered to represent a novel species of the genus Haloactinobacterium, for which the name Haloactinobacterium glacieicola sp.
31208015	7	0	theme	alpha	1111:1115	arg1	parameters					1127:1136	alpha diversity parameters	1111:1136	alpha diversity parameters	1111:1136	After the intervention with prebiotics, we found a drastic decrease in alpha diversity parameters, as well as a change of beta diversity, without a clear directionality toward a healthy microbiota.
31208015	2	1	from	dysbiosis	385:393	arg1	infection					421:429	HIV infection	417:429	HIV infection	417:429	Although, the HIV-associated gut microbiota was studied in depth, few works addressed the dysbiosis of oral microbiota in HIV infection and, to our knowledge, no studies on intervention with prebiotics were performed.
31208015	1	2	theme	early	160:164	arg1	depletion					166:174	an early depletion	157:174	an early depletion of the mucosal associated T helper (CD4+) cells that impair the host immunity and impact the oral and gut microbiomes	157:292	Human immunodeficiency virus (HIV) infection is characterized by an early depletion of the mucosal associated T helper (CD4+) cells that impair the host immunity and impact the oral and gut microbiomes.
31208015	6	3	theme	HIV	952:954	arg1	status					956:961	the HIV status	948:961	the HIV status	948:961	At baseline, the different groups shared the same most abundant genera, but the HIV status had an impact on the saliva microbiota composition and diversity parameters.
31208015	6	4	theme	microbiota	991:1000	arg1	composition					1002:1012	the saliva microbiota composition	980:1012	the saliva microbiota composition	980:1012	At baseline, the different groups shared the same most abundant genera, but the HIV status had an impact on the saliva microbiota composition and diversity parameters.
31208015	9	5	theme	gastrointestinal	1500:1515	arg1	tract					1517:1521	the lower gastrointestinal tract	1490:1521	the lower gastrointestinal tract	1490:1521	On the basis of 100% identity clustering, we detected saliva sequences in the feces datasets, suggesting a drag of microorganisms from the upper to the lower gastrointestinal tract.
31208015	1	6	theme	host	240:243	arg1	immunity					245:252	the host immunity	236:252	the host immunity	236:252	Human immunodeficiency virus (HIV) infection is characterized by an early depletion of the mucosal associated T helper (CD4+) cells that impair the host immunity and impact the oral and gut microbiomes.
31208015	3	7	from	microbiota	595:604	arg1	patients					613:620	HIV patients	609:620	HIV patients	609:620	We studied the effect of a six-week-long prebiotic administration on the salivary microbiota in HIV patients and healthy subjects.
31208015	3	7	from	microbiota	595:604	arg1	subjects					634:641	healthy subjects	626:641	healthy subjects	626:641	We studied the effect of a six-week-long prebiotic administration on the salivary microbiota in HIV patients and healthy subjects.
31208015	7	8	from	decrease	1099:1106	arg1	parameters					1127:1136	alpha diversity parameters	1111:1136	alpha diversity parameters	1111:1136	After the intervention with prebiotics, we found a drastic decrease in alpha diversity parameters, as well as a change of beta diversity, without a clear directionality toward a healthy microbiota.
31208015	6	9	from	impact	970:975	arg1	composition					1002:1012	the saliva microbiota composition	980:1012	the saliva microbiota composition	980:1012	At baseline, the different groups shared the same most abundant genera, but the HIV status had an impact on the saliva microbiota composition and diversity parameters.
31208015	6	9	from	impact	970:975	arg1	parameters					1028:1037	diversity parameters	1018:1037	diversity parameters	1018:1037	At baseline, the different groups shared the same most abundant genera, but the HIV status had an impact on the saliva microbiota composition and diversity parameters.
31208015	4	10	theme	fecal	700:704	arg1	community					715:723	the fecal bacteria community	696:723	the fecal bacteria community	696:723	Also, the co-occurrence of saliva microorganisms in the fecal bacteria community was explored.
31208015	8	11	theme	differential	1264:1275	arg1	response					1277:1284	a differential response	1262:1284	a differential response to the prebiotics	1262:1302	Interestingly, we found a differential response to the prebiotics, depending on the initial microbiota.
31208015	6	12	contain	had	963:965	arg2	impact					970:975	an impact	967:975	an impact on the saliva microbiota composition and diversity parameters	967:1037	At baseline, the different groups shared the same most abundant genera, but the HIV status had an impact on the saliva microbiota composition and diversity parameters.
31208015	6	12	contain	had	963:965	arg1	status					956:961	the HIV status	948:961	the HIV status	948:961	At baseline, the different groups shared the same most abundant genera, but the HIV status had an impact on the saliva microbiota composition and diversity parameters.
31208015	7	13	with	intervention	1050:1061	arg1	prebiotics					1068:1077	prebiotics	1068:1077	prebiotics	1068:1077	After the intervention with prebiotics, we found a drastic decrease in alpha diversity parameters, as well as a change of beta diversity, without a clear directionality toward a healthy microbiota.
31208015	0	14	from	Intervention	50:61	arg1	Individuals					79:89	HIV-Infected Individuals	66:89	HIV-Infected Individuals	66:89	Modulation of Saliva Microbiota through Prebiotic Intervention in HIV-Infected Individuals.
31208015	5	15	theme	ribosomal	808:816	arg1	RNA					818:820	16S ribosomal RNA	804:820	deep 16S ribosomal RNA (rRNA) gene sequencing	799:843	We assessed salivary and feces microbiota composition using deep 16S ribosomal RNA (rRNA) gene sequencing with Illumina methodology.
31208015	5	15	theme	ribosomal	808:816	arg1	rRNA					823:826	rRNA	823:826	rRNA	823:826	We assessed salivary and feces microbiota composition using deep 16S ribosomal RNA (rRNA) gene sequencing with Illumina methodology.
31208015	5	16	theme	microbiota	770:779	arg1	composition					781:791	microbiota composition	770:791	microbiota composition	770:791	We assessed salivary and feces microbiota composition using deep 16S ribosomal RNA (rRNA) gene sequencing with Illumina methodology.
31208015	9	17	theme	microorganisms	1457:1470	arg1	drag					1449:1452	a drag	1447:1452	a drag of microorganisms from the upper to the lower gastrointestinal tract	1447:1521	On the basis of 100% identity clustering, we detected saliva sequences in the feces datasets, suggesting a drag of microorganisms from the upper to the lower gastrointestinal tract.
31208015	5	18	theme	gene	829:832	arg1	sequencing					834:843	deep 16S ribosomal RNA (rRNA) gene sequencing	799:843	deep 16S ribosomal RNA (rRNA) gene sequencing	799:843	We assessed salivary and feces microbiota composition using deep 16S ribosomal RNA (rRNA) gene sequencing with Illumina methodology.
31208015	6	19	theme	saliva	984:989	arg1	composition					1002:1012	the saliva microbiota composition	980:1012	the saliva microbiota composition	980:1012	At baseline, the different groups shared the same most abundant genera, but the HIV status had an impact on the saliva microbiota composition and diversity parameters.
31208015	7	20	theme	drastic	1091:1097	arg1	decrease					1099:1106	a drastic decrease	1089:1106	a drastic decrease in alpha diversity parameters	1089:1136	After the intervention with prebiotics, we found a drastic decrease in alpha diversity parameters, as well as a change of beta diversity, without a clear directionality toward a healthy microbiota.
31208015	1	21	theme	Human	92:96	arg1	virus					115:119	Human immunodeficiency virus	92:119	Human immunodeficiency virus (HIV) infection	92:135	Human immunodeficiency virus (HIV) infection is characterized by an early depletion of the mucosal associated T helper (CD4+) cells that impair the host immunity and impact the oral and gut microbiomes.
31208015	1	21	theme	Human	92:96	arg1	HIV					122:124	HIV	122:124	HIV	122:124	Human immunodeficiency virus (HIV) infection is characterized by an early depletion of the mucosal associated T helper (CD4+) cells that impair the host immunity and impact the oral and gut microbiomes.
31208015	2	22	theme	few	361:363	arg1	works					365:369	few works	361:369	few works	361:369	Although, the HIV-associated gut microbiota was studied in depth, few works addressed the dysbiosis of oral microbiota in HIV infection and, to our knowledge, no studies on intervention with prebiotics were performed.
31208015	2	23	theme	HIV	417:419	arg1	infection					421:429	HIV infection	417:429	HIV infection	417:429	Although, the HIV-associated gut microbiota was studied in depth, few works addressed the dysbiosis of oral microbiota in HIV infection and, to our knowledge, no studies on intervention with prebiotics were performed.
31208015	2	24	with	studies	457:463	arg1	prebiotics					486:495	prebiotics	486:495	prebiotics	486:495	Although, the HIV-associated gut microbiota was studied in depth, few works addressed the dysbiosis of oral microbiota in HIV infection and, to our knowledge, no studies on intervention with prebiotics were performed.
31208015	1	25	theme	immunodeficiency	98:113	arg1	virus					115:119	Human immunodeficiency virus	92:119	Human immunodeficiency virus (HIV) infection	92:135	Human immunodeficiency virus (HIV) infection is characterized by an early depletion of the mucosal associated T helper (CD4+) cells that impair the host immunity and impact the oral and gut microbiomes.
31208015	1	25	theme	immunodeficiency	98:113	arg1	HIV					122:124	HIV	122:124	HIV	122:124	Human immunodeficiency virus (HIV) infection is characterized by an early depletion of the mucosal associated T helper (CD4+) cells that impair the host immunity and impact the oral and gut microbiomes.
31208015	7	26	theme	diversity	1117:1125	arg1	parameters					1127:1136	alpha diversity parameters	1111:1136	alpha diversity parameters	1111:1136	After the intervention with prebiotics, we found a drastic decrease in alpha diversity parameters, as well as a change of beta diversity, without a clear directionality toward a healthy microbiota.
31208015	1	27	theme	mucosal	183:189	arg1	cells					218:222	the mucosal associated T helper (CD4+) cells	179:222	the mucosal associated T helper (CD4+) cells that impair the host immunity and impact the oral and gut microbiomes	179:292	Human immunodeficiency virus (HIV) infection is characterized by an early depletion of the mucosal associated T helper (CD4+) cells that impair the host immunity and impact the oral and gut microbiomes.
31208015	7	28	theme	diversity	1167:1175	arg1	change					1152:1157	a change	1150:1157	a change of beta diversity	1150:1175	After the intervention with prebiotics, we found a drastic decrease in alpha diversity parameters, as well as a change of beta diversity, without a clear directionality toward a healthy microbiota.
31208015	7	28	theme	diversity	1167:1175	arg1	decrease					1099:1106	a drastic decrease	1089:1106	a drastic decrease in alpha diversity parameters	1089:1136	After the intervention with prebiotics, we found a drastic decrease in alpha diversity parameters, as well as a change of beta diversity, without a clear directionality toward a healthy microbiota.
31208015	5	29	dep	salivary	751:758	arg1	composition					781:791	microbiota composition	770:791	microbiota composition	770:791	We assessed salivary and feces microbiota composition using deep 16S ribosomal RNA (rRNA) gene sequencing with Illumina methodology.
31208015	1	30	theme	virus	115:119	arg1	infection					127:135	Human immunodeficiency virus (HIV) infection	92:135	Human immunodeficiency virus (HIV) infection	92:135	Human immunodeficiency virus (HIV) infection is characterized by an early depletion of the mucosal associated T helper (CD4+) cells that impair the host immunity and impact the oral and gut microbiomes.
31208015	1	31	theme	associated	191:200	arg1	cells					218:222	the mucosal associated T helper (CD4+) cells	179:222	the mucosal associated T helper (CD4+) cells that impair the host immunity and impact the oral and gut microbiomes	179:292	Human immunodeficiency virus (HIV) infection is characterized by an early depletion of the mucosal associated T helper (CD4+) cells that impair the host immunity and impact the oral and gut microbiomes.
31208015	0	32	theme	Microbiota	21:30	arg1	Modulation					0:9	Modulation	0:9	Modulation of Saliva Microbiota through Prebiotic Intervention in HIV-Infected Individuals.	0:90	Modulation of Saliva Microbiota through Prebiotic Intervention in HIV-Infected Individuals.
31208015	6	33	theme	diversity	1018:1026	arg1	parameters					1028:1037	diversity parameters	1018:1037	diversity parameters	1018:1037	At baseline, the different groups shared the same most abundant genera, but the HIV status had an impact on the saliva microbiota composition and diversity parameters.
31208015	8	34	theme	initial	1322:1328	arg1	microbiota					1330:1339	the initial microbiota	1318:1339	the initial microbiota	1318:1339	Interestingly, we found a differential response to the prebiotics, depending on the initial microbiota.
31208015	1	35	theme	T	202:202	arg1	cells					218:222	the mucosal associated T helper (CD4+) cells	179:222	the mucosal associated T helper (CD4+) cells that impair the host immunity and impact the oral and gut microbiomes	179:292	Human immunodeficiency virus (HIV) infection is characterized by an early depletion of the mucosal associated T helper (CD4+) cells that impair the host immunity and impact the oral and gut microbiomes.
31208015	2	36	theme	oral	398:401	arg1	microbiota					403:412	oral microbiota	398:412	oral microbiota	398:412	Although, the HIV-associated gut microbiota was studied in depth, few works addressed the dysbiosis of oral microbiota in HIV infection and, to our knowledge, no studies on intervention with prebiotics were performed.
31208015	1	37	theme	oral	269:272	arg1	microbiomes					282:292	the oral and gut microbiomes	265:292	microbiomes	282:292	Human immunodeficiency virus (HIV) infection is characterized by an early depletion of the mucosal associated T helper (CD4+) cells that impair the host immunity and impact the oral and gut microbiomes.
31208015	3	38	theme	HIV	609:611	arg1	patients					613:620	HIV patients	609:620	HIV patients	609:620	We studied the effect of a six-week-long prebiotic administration on the salivary microbiota in HIV patients and healthy subjects.
31208015	4	39	theme	bacteria	706:713	arg1	community					715:723	the fecal bacteria community	696:723	the fecal bacteria community	696:723	Also, the co-occurrence of saliva microorganisms in the fecal bacteria community was explored.
31208015	1	40	theme	helper	204:209	arg1	cells					218:222	the mucosal associated T helper (CD4+) cells	179:222	the mucosal associated T helper (CD4+) cells that impair the host immunity and impact the oral and gut microbiomes	179:292	Human immunodeficiency virus (HIV) infection is characterized by an early depletion of the mucosal associated T helper (CD4+) cells that impair the host immunity and impact the oral and gut microbiomes.
31208015	0	41	theme	Prebiotic	40:48	arg1	Intervention					50:61	Prebiotic Intervention	40:61	Prebiotic Intervention in HIV-Infected Individuals	40:89	Modulation of Saliva Microbiota through Prebiotic Intervention in HIV-Infected Individuals.
31208015	6	42	theme	same	917:920	arg1	genera					936:941	the same most abundant genera	913:941	the same most abundant genera	913:941	At baseline, the different groups shared the same most abundant genera, but the HIV status had an impact on the saliva microbiota composition and diversity parameters.
31208015	7	43	theme	healthy	1218:1224	arg1	microbiota					1226:1235	a healthy microbiota	1216:1235	a healthy microbiota	1216:1235	After the intervention with prebiotics, we found a drastic decrease in alpha diversity parameters, as well as a change of beta diversity, without a clear directionality toward a healthy microbiota.
31208015	3	44	theme	six-week-long	540:552	arg1	administration					564:577	a six-week-long prebiotic administration	538:577	a six-week-long prebiotic administration	538:577	We studied the effect of a six-week-long prebiotic administration on the salivary microbiota in HIV patients and healthy subjects.
31208015	9	45	from	upper	1481:1485	arg1	drag					1449:1452	a drag	1447:1452	a drag of microorganisms from the upper to the lower gastrointestinal tract	1447:1521	On the basis of 100% identity clustering, we detected saliva sequences in the feces datasets, suggesting a drag of microorganisms from the upper to the lower gastrointestinal tract.
31208015	5	46	theme	RNA	818:820	arg1	sequencing					834:843	deep 16S ribosomal RNA (rRNA) gene sequencing	799:843	deep 16S ribosomal RNA (rRNA) gene sequencing	799:843	We assessed salivary and feces microbiota composition using deep 16S ribosomal RNA (rRNA) gene sequencing with Illumina methodology.
31208015	9	47	theme	100	1358:1360	arg1	%					1361:1361	%	1361:1361	%	1361:1361	On the basis of 100% identity clustering, we detected saliva sequences in the feces datasets, suggesting a drag of microorganisms from the upper to the lower gastrointestinal tract.
31208015	1	48	theme	gut	278:280	arg1	microbiomes					282:292	the oral and gut microbiomes	265:292	microbiomes	282:292	Human immunodeficiency virus (HIV) infection is characterized by an early depletion of the mucosal associated T helper (CD4+) cells that impair the host immunity and impact the oral and gut microbiomes.
31208015	9	49	theme	saliva	1396:1401	arg1	sequences					1403:1411	saliva sequences	1396:1411	saliva sequences	1396:1411	On the basis of 100% identity clustering, we detected saliva sequences in the feces datasets, suggesting a drag of microorganisms from the upper to the lower gastrointestinal tract.
31208015	2	50	theme	microbiota	403:412	arg1	dysbiosis					385:393	the dysbiosis	381:393	the dysbiosis of oral microbiota in HIV infection	381:429	Although, the HIV-associated gut microbiota was studied in depth, few works addressed the dysbiosis of oral microbiota in HIV infection and, to our knowledge, no studies on intervention with prebiotics were performed.
31208015	1	51	theme	CD4+	212:215	arg1	cells					218:222	the mucosal associated T helper (CD4+) cells	179:222	the mucosal associated T helper (CD4+) cells that impair the host immunity and impact the oral and gut microbiomes	179:292	Human immunodeficiency virus (HIV) infection is characterized by an early depletion of the mucosal associated T helper (CD4+) cells that impair the host immunity and impact the oral and gut microbiomes.
31208015	9	52	dep	clustering	1372:1381	arg1	the					1345:1347	the	1345:1347	the	1345:1347	On the basis of 100% identity clustering, we detected saliva sequences in the feces datasets, suggesting a drag of microorganisms from the upper to the lower gastrointestinal tract.
31208015	9	52	dep	clustering	1372:1381	arg1	basis					1349:1353	basis	1349:1353	basis	1349:1353	On the basis of 100% identity clustering, we detected saliva sequences in the feces datasets, suggesting a drag of microorganisms from the upper to the lower gastrointestinal tract.
31208015	9	53	theme	%	1361:1361	arg1	clustering					1372:1381	100% identity clustering	1358:1381	100% identity clustering	1358:1381	On the basis of 100% identity clustering, we detected saliva sequences in the feces datasets, suggesting a drag of microorganisms from the upper to the lower gastrointestinal tract.
31208015	5	54	theme	deep	799:802	arg1	sequencing					834:843	deep 16S ribosomal RNA (rRNA) gene sequencing	799:843	deep 16S ribosomal RNA (rRNA) gene sequencing	799:843	We assessed salivary and feces microbiota composition using deep 16S ribosomal RNA (rRNA) gene sequencing with Illumina methodology.
31208015	6	55	theme	abundant	927:934	arg1	genera					936:941	the same most abundant genera	913:941	the same most abundant genera	913:941	At baseline, the different groups shared the same most abundant genera, but the HIV status had an impact on the saliva microbiota composition and diversity parameters.
31208015	2	56	theme	gut	324:326	arg1	microbiota					328:337	the HIV-associated gut microbiota	305:337	the HIV-associated gut microbiota	305:337	Although, the HIV-associated gut microbiota was studied in depth, few works addressed the dysbiosis of oral microbiota in HIV infection and, to our knowledge, no studies on intervention with prebiotics were performed.
31208015	9	57	theme	feces	1420:1424	arg1	datasets					1426:1433	the feces datasets	1416:1433	the feces datasets	1416:1433	On the basis of 100% identity clustering, we detected saliva sequences in the feces datasets, suggesting a drag of microorganisms from the upper to the lower gastrointestinal tract.
31208015	7	58	from	change	1152:1157	arg1	parameters					1127:1136	alpha diversity parameters	1111:1136	alpha diversity parameters	1111:1136	After the intervention with prebiotics, we found a drastic decrease in alpha diversity parameters, as well as a change of beta diversity, without a clear directionality toward a healthy microbiota.
31208015	2	59	theme	HIV-associated	309:322	arg1	microbiota					328:337	the HIV-associated gut microbiota	305:337	the HIV-associated gut microbiota	305:337	Although, the HIV-associated gut microbiota was studied in depth, few works addressed the dysbiosis of oral microbiota in HIV infection and, to our knowledge, no studies on intervention with prebiotics were performed.
31208015	6	60	theme	different	889:897	arg1	groups					899:904	the different groups	885:904	the different groups	885:904	At baseline, the different groups shared the same most abundant genera, but the HIV status had an impact on the saliva microbiota composition and diversity parameters.
31208015	4	61	theme	saliva	671:676	arg1	microorganisms					678:691	saliva microorganisms	671:691	saliva microorganisms	671:691	Also, the co-occurrence of saliva microorganisms in the fecal bacteria community was explored.
31208015	2	62	from	studies	457:463	arg1	intervention					468:479	intervention	468:479	intervention	468:479	Although, the HIV-associated gut microbiota was studied in depth, few works addressed the dysbiosis of oral microbiota in HIV infection and, to our knowledge, no studies on intervention with prebiotics were performed.
31208015	3	63	theme	prebiotic	554:562	arg1	administration					564:577	a six-week-long prebiotic administration	538:577	a six-week-long prebiotic administration	538:577	We studied the effect of a six-week-long prebiotic administration on the salivary microbiota in HIV patients and healthy subjects.
31208015	7	64	theme	clear	1188:1192	arg1	directionality					1194:1207	a clear directionality	1186:1207	a clear directionality toward a healthy microbiota	1186:1235	After the intervention with prebiotics, we found a drastic decrease in alpha diversity parameters, as well as a change of beta diversity, without a clear directionality toward a healthy microbiota.
31208015	3	65	theme	salivary	586:593	arg1	microbiota					595:604	the salivary microbiota	582:604	the salivary microbiota in HIV patients and healthy subjects	582:641	We studied the effect of a six-week-long prebiotic administration on the salivary microbiota in HIV patients and healthy subjects.
31208015	4	66	theme	microorganisms	678:691	arg1	co-occurrence					654:666	the co-occurrence	650:666	the co-occurrence of saliva microorganisms in the fecal bacteria community	650:723	Also, the co-occurrence of saliva microorganisms in the fecal bacteria community was explored.
31208015	1	67	theme	cells	218:222	arg1	depletion					166:174	an early depletion	157:174	an early depletion of the mucosal associated T helper (CD4+) cells that impair the host immunity and impact the oral and gut microbiomes	157:292	Human immunodeficiency virus (HIV) infection is characterized by an early depletion of the mucosal associated T helper (CD4+) cells that impair the host immunity and impact the oral and gut microbiomes.
31208015	7	68	theme	beta	1162:1165	arg1	diversity					1167:1175	beta diversity	1162:1175	beta diversity	1162:1175	After the intervention with prebiotics, we found a drastic decrease in alpha diversity parameters, as well as a change of beta diversity, without a clear directionality toward a healthy microbiota.
31208015	3	69	theme	healthy	626:632	arg1	subjects					634:641	healthy subjects	626:641	healthy subjects	626:641	We studied the effect of a six-week-long prebiotic administration on the salivary microbiota in HIV patients and healthy subjects.
31208015	3	70	theme	administration	564:577	arg1	effect					528:533	the effect	524:533	the effect of a six-week-long prebiotic administration on the salivary microbiota in HIV patients and healthy subjects	524:641	We studied the effect of a six-week-long prebiotic administration on the salivary microbiota in HIV patients and healthy subjects.
31208015	5	71	theme	16S	804:806	arg1	RNA					818:820	16S ribosomal RNA	804:820	deep 16S ribosomal RNA (rRNA) gene sequencing	799:843	We assessed salivary and feces microbiota composition using deep 16S ribosomal RNA (rRNA) gene sequencing with Illumina methodology.
31208015	5	71	theme	16S	804:806	arg1	rRNA					823:826	rRNA	823:826	rRNA	823:826	We assessed salivary and feces microbiota composition using deep 16S ribosomal RNA (rRNA) gene sequencing with Illumina methodology.
31208015	3	72	from	effect	528:533	arg1	microbiota					595:604	the salivary microbiota	582:604	the salivary microbiota in HIV patients and healthy subjects	582:641	We studied the effect of a six-week-long prebiotic administration on the salivary microbiota in HIV patients and healthy subjects.
31208015	9	73	theme	lower	1494:1498	arg1	tract					1517:1521	the lower gastrointestinal tract	1490:1521	the lower gastrointestinal tract	1490:1521	On the basis of 100% identity clustering, we detected saliva sequences in the feces datasets, suggesting a drag of microorganisms from the upper to the lower gastrointestinal tract.
31208015	0	74	theme	HIV-Infected	66:77	arg1	Individuals					79:89	HIV-Infected Individuals	66:89	HIV-Infected Individuals	66:89	Modulation of Saliva Microbiota through Prebiotic Intervention in HIV-Infected Individuals.
31208015	9	75	theme	identity	1363:1370	arg1	clustering					1372:1381	100% identity clustering	1358:1381	100% identity clustering	1358:1381	On the basis of 100% identity clustering, we detected saliva sequences in the feces datasets, suggesting a drag of microorganisms from the upper to the lower gastrointestinal tract.
31208015	0	76	theme	Saliva	14:19	arg1	Microbiota					21:30	Saliva Microbiota	14:30	Saliva Microbiota	14:30	Modulation of Saliva Microbiota through Prebiotic Intervention in HIV-Infected Individuals.
31208015	5	77	theme	Illumina	850:857	arg1	methodology					859:869	Illumina methodology	850:869	Illumina methodology	850:869	We assessed salivary and feces microbiota composition using deep 16S ribosomal RNA (rRNA) gene sequencing with Illumina methodology.
31208015	4	78	from	co-occurrence	654:666	arg1	community					715:723	the fecal bacteria community	696:723	the fecal bacteria community	696:723	Also, the co-occurrence of saliva microorganisms in the fecal bacteria community was explored.
30471564	0	0	theme	C57BL/6	84:90	arg1	mice					94:97	C57BL/6 J mice	84:97	C57BL/6 J mice	84:97	Blueberry polyphenols extract as a potential prebiotic with anti-obesity effects on C57BL/6 J mice by modulating the gut microbiota.
30471564	8	1	theme	C57BL/6	978:984	arg1	mice					988:991	C57BL/6 J mice	978:991	C57BL/6 J mice	978:991	Furthermore, 16S rRNA gene sequencing of the fecal microbiota suggested that PPE changed the composition of the gut microbiota in C57BL/6 J mice and modulated specific bacteria such as Proteobacteria, Deferribacteres, Actinobacteria, Bifidobacterium, Desulfovibrio, Adlercreutzia, Helicobacter, Flexispira, and Prevotella.
30471564	5	2	theme	J	572:572	arg1	mice					574:577	Four-week-old C57BL/6 J mice	550:577	Four-week-old C57BL/6 J mice	550:577	Four-week-old C57BL/6 J mice were fed a normal-fat diet or HFD with or without PPE or Orlistat for 12 weeks.
30471564	4	3	theme	J	506:506	arg1	mice					508:511	C57BL/6 J mice	498:511	C57BL/6 J mice	498:511	This study investigated the potential positive effect of blueberry polyphenol extract (PPE) on high-fat diet (HFD)-induced obesity in C57BL/6 J mice by modulation of the gut microbiota.
30471564	6	4	theme	disordered	734:743	arg1	metabolism					751:760	disordered lipid metabolism	734:760	disordered lipid metabolism	734:760	Mice fed HFD exhibited increased body weight and adipose tissue weight and disordered lipid metabolism.
30471564	8	5	theme	J	986:986	arg1	mice					988:991	C57BL/6 J mice	978:991	C57BL/6 J mice	978:991	Furthermore, 16S rRNA gene sequencing of the fecal microbiota suggested that PPE changed the composition of the gut microbiota in C57BL/6 J mice and modulated specific bacteria such as Proteobacteria, Deferribacteres, Actinobacteria, Bifidobacterium, Desulfovibrio, Adlercreutzia, Helicobacter, Flexispira, and Prevotella.
30471564	10	6	theme	HFD-induced	1407:1417	arg1	obesity					1419:1425	HFD-induced obesity	1407:1425	HFD-induced obesity in C57BL/6 J mice	1407:1443	Our findings suggest that PPE, as a potential prebiotic agent, influences the gut microbiota to positively affect HFD-induced obesity in C57BL/6 J mice.
30471564	4	7	theme	C57BL/6	498:504	arg1	mice					508:511	C57BL/6 J mice	498:511	C57BL/6 J mice	498:511	This study investigated the potential positive effect of blueberry polyphenol extract (PPE) on high-fat diet (HFD)-induced obesity in C57BL/6 J mice by modulation of the gut microbiota.
30471564	8	8	theme	fecal	893:897	arg1	microbiota					899:908	the fecal microbiota	889:908	the fecal microbiota	889:908	Furthermore, 16S rRNA gene sequencing of the fecal microbiota suggested that PPE changed the composition of the gut microbiota in C57BL/6 J mice and modulated specific bacteria such as Proteobacteria, Deferribacteres, Actinobacteria, Bifidobacterium, Desulfovibrio, Adlercreutzia, Helicobacter, Flexispira, and Prevotella.
30471564	4	9	theme	positive	402:409	arg1	effect					411:416	the potential positive effect	388:416	the potential positive effect of blueberry polyphenol extract (PPE) on high-fat diet (HFD)-induced obesity in C57BL/6 J mice	388:511	This study investigated the potential positive effect of blueberry polyphenol extract (PPE) on high-fat diet (HFD)-induced obesity in C57BL/6 J mice by modulation of the gut microbiota.
30471564	0	10	theme	J	92:92	arg1	mice					94:97	C57BL/6 J mice	84:97	C57BL/6 J mice	84:97	Blueberry polyphenols extract as a potential prebiotic with anti-obesity effects on C57BL/6 J mice by modulating the gut microbiota.
30471564	4	11	from	obesity	487:493	arg1	mice					508:511	C57BL/6 J mice	498:511	C57BL/6 J mice	498:511	This study investigated the potential positive effect of blueberry polyphenol extract (PPE) on high-fat diet (HFD)-induced obesity in C57BL/6 J mice by modulation of the gut microbiota.
30471564	4	12	theme	blueberry	421:429	arg1	PPE					451:453	PPE	451:453	PPE	451:453	This study investigated the potential positive effect of blueberry polyphenol extract (PPE) on high-fat diet (HFD)-induced obesity in C57BL/6 J mice by modulation of the gut microbiota.
30471564	4	12	theme	blueberry	421:429	arg1	extract					442:448	blueberry polyphenol extract	421:448	blueberry polyphenol extract (PPE)	421:454	This study investigated the potential positive effect of blueberry polyphenol extract (PPE) on high-fat diet (HFD)-induced obesity in C57BL/6 J mice by modulation of the gut microbiota.
30471564	9	13	theme	gut	1277:1279	arg1	microbiota					1281:1290	the gut microbiota	1273:1290	the gut microbiota	1273:1290	Orlistat also improved obesity and metabolic alterations of HFD mice and modulated the composition of the gut microbiota.
30471564	10	14	theme	J	1438:1438	arg1	mice					1440:1443	C57BL/6 J mice	1430:1443	C57BL/6 J mice	1430:1443	Our findings suggest that PPE, as a potential prebiotic agent, influences the gut microbiota to positively affect HFD-induced obesity in C57BL/6 J mice.
30471564	10	15	theme	potential	1329:1337	arg1	agent					1349:1353	a potential prebiotic agent	1327:1353	a potential prebiotic agent	1327:1353	Our findings suggest that PPE, as a potential prebiotic agent, influences the gut microbiota to positively affect HFD-induced obesity in C57BL/6 J mice.
30471564	10	15	theme	potential	1329:1337	arg1	microbiota					1375:1384	the gut microbiota	1367:1384	the gut microbiota to positively affect HFD-induced obesity in C57BL/6 J mice	1367:1443	Our findings suggest that PPE, as a potential prebiotic agent, influences the gut microbiota to positively affect HFD-induced obesity in C57BL/6 J mice.
30471564	4	16	from	effect	411:416	arg1	obesity					487:493	high-fat diet (HFD)-induced obesity	459:493	high-fat diet (HFD)-induced obesity in C57BL/6 J mice	459:511	This study investigated the potential positive effect of blueberry polyphenol extract (PPE) on high-fat diet (HFD)-induced obesity in C57BL/6 J mice by modulation of the gut microbiota.
30471564	7	17	theme	lipid	820:824	arg1	metabolism					826:835	lipid metabolism	820:835	lipid metabolism	820:835	In contrast, PPE inhibited body weight gain and returned lipid metabolism to normal.
30471564	4	18	theme	-induced	478:485	arg1	obesity					487:493	high-fat diet (HFD)-induced obesity	459:493	high-fat diet (HFD)-induced obesity in C57BL/6 J mice	459:511	This study investigated the potential positive effect of blueberry polyphenol extract (PPE) on high-fat diet (HFD)-induced obesity in C57BL/6 J mice by modulation of the gut microbiota.
30471564	6	19	theme	lipid	745:749	arg1	metabolism					751:760	disordered lipid metabolism	734:760	disordered lipid metabolism	734:760	Mice fed HFD exhibited increased body weight and adipose tissue weight and disordered lipid metabolism.
30471564	5	20	theme	Four-week-old	550:562	arg1	mice					574:577	Four-week-old C57BL/6 J mice	550:577	Four-week-old C57BL/6 J mice	550:577	Four-week-old C57BL/6 J mice were fed a normal-fat diet or HFD with or without PPE or Orlistat for 12 weeks.
30471564	1	21	theme	various	165:171	arg1	benefits					180:187	their various health benefits	159:187	their various health benefits	159:187	Polyphenols are known for their various health benefits.
30471564	5	22	theme	C57BL/6	564:570	arg1	mice					574:577	Four-week-old C57BL/6 J mice	550:577	Four-week-old C57BL/6 J mice	550:577	Four-week-old C57BL/6 J mice were fed a normal-fat diet or HFD with or without PPE or Orlistat for 12 weeks.
30471564	1	23	theme	health	173:178	arg1	benefits					180:187	their various health benefits	159:187	their various health benefits	159:187	Polyphenols are known for their various health benefits.
30471564	0	24	theme	Blueberry	0:8	arg1	polyphenols					10:20	Blueberry polyphenols	0:20	Blueberry polyphenols	0:20	Blueberry polyphenols extract as a potential prebiotic with anti-obesity effects on C57BL/6 J mice by modulating the gut microbiota.
30471564	4	25	theme	gut	534:536	arg1	microbiota					538:547	the gut microbiota	530:547	the gut microbiota	530:547	This study investigated the potential positive effect of blueberry polyphenol extract (PPE) on high-fat diet (HFD)-induced obesity in C57BL/6 J mice by modulation of the gut microbiota.
30471564	8	26	theme	specific	1007:1014	arg1	Prevotella					1159:1168	Prevotella	1159:1168	Prevotella	1159:1168	Furthermore, 16S rRNA gene sequencing of the fecal microbiota suggested that PPE changed the composition of the gut microbiota in C57BL/6 J mice and modulated specific bacteria such as Proteobacteria, Deferribacteres, Actinobacteria, Bifidobacterium, Desulfovibrio, Adlercreutzia, Helicobacter, Flexispira, and Prevotella.
30471564	8	26	theme	specific	1007:1014	arg1	Actinobacteria					1066:1079	Actinobacteria	1066:1079	Actinobacteria	1066:1079	Furthermore, 16S rRNA gene sequencing of the fecal microbiota suggested that PPE changed the composition of the gut microbiota in C57BL/6 J mice and modulated specific bacteria such as Proteobacteria, Deferribacteres, Actinobacteria, Bifidobacterium, Desulfovibrio, Adlercreutzia, Helicobacter, Flexispira, and Prevotella.
30471564	8	26	theme	specific	1007:1014	arg1	Adlercreutzia					1114:1126	Adlercreutzia	1114:1126	Adlercreutzia	1114:1126	Furthermore, 16S rRNA gene sequencing of the fecal microbiota suggested that PPE changed the composition of the gut microbiota in C57BL/6 J mice and modulated specific bacteria such as Proteobacteria, Deferribacteres, Actinobacteria, Bifidobacterium, Desulfovibrio, Adlercreutzia, Helicobacter, Flexispira, and Prevotella.
30471564	8	26	theme	specific	1007:1014	arg1	Proteobacteria					1033:1046	Proteobacteria	1033:1046	Proteobacteria	1033:1046	Furthermore, 16S rRNA gene sequencing of the fecal microbiota suggested that PPE changed the composition of the gut microbiota in C57BL/6 J mice and modulated specific bacteria such as Proteobacteria, Deferribacteres, Actinobacteria, Bifidobacterium, Desulfovibrio, Adlercreutzia, Helicobacter, Flexispira, and Prevotella.
30471564	8	26	theme	specific	1007:1014	arg1	Bifidobacterium					1082:1096	Bifidobacterium	1082:1096	Bifidobacterium	1082:1096	Furthermore, 16S rRNA gene sequencing of the fecal microbiota suggested that PPE changed the composition of the gut microbiota in C57BL/6 J mice and modulated specific bacteria such as Proteobacteria, Deferribacteres, Actinobacteria, Bifidobacterium, Desulfovibrio, Adlercreutzia, Helicobacter, Flexispira, and Prevotella.
30471564	8	26	theme	specific	1007:1014	arg1	Desulfovibrio					1099:1111	Desulfovibrio	1099:1111	Desulfovibrio	1099:1111	Furthermore, 16S rRNA gene sequencing of the fecal microbiota suggested that PPE changed the composition of the gut microbiota in C57BL/6 J mice and modulated specific bacteria such as Proteobacteria, Deferribacteres, Actinobacteria, Bifidobacterium, Desulfovibrio, Adlercreutzia, Helicobacter, Flexispira, and Prevotella.
30471564	8	26	theme	specific	1007:1014	arg1	Flexispira					1143:1152	Flexispira	1143:1152	Flexispira	1143:1152	Furthermore, 16S rRNA gene sequencing of the fecal microbiota suggested that PPE changed the composition of the gut microbiota in C57BL/6 J mice and modulated specific bacteria such as Proteobacteria, Deferribacteres, Actinobacteria, Bifidobacterium, Desulfovibrio, Adlercreutzia, Helicobacter, Flexispira, and Prevotella.
30471564	8	26	theme	specific	1007:1014	arg1	bacteria					1016:1023	specific bacteria	1007:1023	specific bacteria such as Proteobacteria	1007:1046	Furthermore, 16S rRNA gene sequencing of the fecal microbiota suggested that PPE changed the composition of the gut microbiota in C57BL/6 J mice and modulated specific bacteria such as Proteobacteria, Deferribacteres, Actinobacteria, Bifidobacterium, Desulfovibrio, Adlercreutzia, Helicobacter, Flexispira, and Prevotella.
30471564	4	27	theme	potential	392:400	arg1	effect					411:416	the potential positive effect	388:416	the potential positive effect of blueberry polyphenol extract (PPE) on high-fat diet (HFD)-induced obesity in C57BL/6 J mice	388:511	This study investigated the potential positive effect of blueberry polyphenol extract (PPE) on high-fat diet (HFD)-induced obesity in C57BL/6 J mice by modulation of the gut microbiota.
30471564	4	28	theme	extract	442:448	arg1	effect					411:416	the potential positive effect	388:416	the potential positive effect of blueberry polyphenol extract (PPE) on high-fat diet (HFD)-induced obesity in C57BL/6 J mice	388:511	This study investigated the potential positive effect of blueberry polyphenol extract (PPE) on high-fat diet (HFD)-induced obesity in C57BL/6 J mice by modulation of the gut microbiota.
30471564	7	29	theme	body	790:793	arg1	gain					802:805	body weight gain	790:805	body weight gain	790:805	In contrast, PPE inhibited body weight gain and returned lipid metabolism to normal.
30471564	8	30	theme	microbiota	964:973	arg1	composition					941:951	the composition	937:951	the composition of the gut microbiota in C57BL/6 J mice	937:991	Furthermore, 16S rRNA gene sequencing of the fecal microbiota suggested that PPE changed the composition of the gut microbiota in C57BL/6 J mice and modulated specific bacteria such as Proteobacteria, Deferribacteres, Actinobacteria, Bifidobacterium, Desulfovibrio, Adlercreutzia, Helicobacter, Flexispira, and Prevotella.
30471564	2	31	with	sources	214:220	arg1	benefits					258:265	reported health benefits	242:265	reported health benefits	242:265	Blueberries are dietary sources of polyphenols with reported health benefits.
30471564	0	32	theme	gut	117:119	arg1	microbiota					121:130	the gut microbiota	113:130	the gut microbiota	113:130	Blueberry polyphenols extract as a potential prebiotic with anti-obesity effects on C57BL/6 J mice by modulating the gut microbiota.
30471564	8	33	theme	gut	960:962	arg1	microbiota					964:973	the gut microbiota	956:973	the gut microbiota	956:973	Furthermore, 16S rRNA gene sequencing of the fecal microbiota suggested that PPE changed the composition of the gut microbiota in C57BL/6 J mice and modulated specific bacteria such as Proteobacteria, Deferribacteres, Actinobacteria, Bifidobacterium, Desulfovibrio, Adlercreutzia, Helicobacter, Flexispira, and Prevotella.
30471564	7	34	theme	weight	795:800	arg1	gain					802:805	body weight gain	790:805	body weight gain	790:805	In contrast, PPE inhibited body weight gain and returned lipid metabolism to normal.
30471564	6	35	theme	body	692:695	arg1	weight					697:702	increased body weight	682:702	increased body weight	682:702	Mice fed HFD exhibited increased body weight and adipose tissue weight and disordered lipid metabolism.
30471564	8	36	theme	rRNA	865:868	arg1	sequencing					875:884	16S rRNA gene sequencing	861:884	16S rRNA gene sequencing of the fecal microbiota	861:908	Furthermore, 16S rRNA gene sequencing of the fecal microbiota suggested that PPE changed the composition of the gut microbiota in C57BL/6 J mice and modulated specific bacteria such as Proteobacteria, Deferribacteres, Actinobacteria, Bifidobacterium, Desulfovibrio, Adlercreutzia, Helicobacter, Flexispira, and Prevotella.
30471564	10	37	theme	gut	1371:1373	arg1	agent					1349:1353	a potential prebiotic agent	1327:1353	a potential prebiotic agent	1327:1353	Our findings suggest that PPE, as a potential prebiotic agent, influences the gut microbiota to positively affect HFD-induced obesity in C57BL/6 J mice.
30471564	10	37	theme	gut	1371:1373	arg1	microbiota					1375:1384	the gut microbiota	1367:1384	the gut microbiota to positively affect HFD-induced obesity in C57BL/6 J mice	1367:1443	Our findings suggest that PPE, as a potential prebiotic agent, influences the gut microbiota to positively affect HFD-induced obesity in C57BL/6 J mice.
30471564	0	38	theme	potential	35:43	arg1	prebiotic					45:53	a potential prebiotic	33:53	a potential prebiotic with anti-obesity effects on C57BL/6 J mice	33:97	Blueberry polyphenols extract as a potential prebiotic with anti-obesity effects on C57BL/6 J mice by modulating the gut microbiota.
30471564	4	39	theme	microbiota	538:547	arg1	modulation					516:525	modulation	516:525	modulation of the gut microbiota	516:547	This study investigated the potential positive effect of blueberry polyphenol extract (PPE) on high-fat diet (HFD)-induced obesity in C57BL/6 J mice by modulation of the gut microbiota.
30471564	8	40	theme	16S	861:863	arg1	sequencing					875:884	16S rRNA gene sequencing	861:884	16S rRNA gene sequencing of the fecal microbiota	861:908	Furthermore, 16S rRNA gene sequencing of the fecal microbiota suggested that PPE changed the composition of the gut microbiota in C57BL/6 J mice and modulated specific bacteria such as Proteobacteria, Deferribacteres, Actinobacteria, Bifidobacterium, Desulfovibrio, Adlercreutzia, Helicobacter, Flexispira, and Prevotella.
30471564	0	41	from	effects	73:79	arg1	mice					94:97	C57BL/6 J mice	84:97	C57BL/6 J mice	84:97	Blueberry polyphenols extract as a potential prebiotic with anti-obesity effects on C57BL/6 J mice by modulating the gut microbiota.
30471564	2	42	theme	polyphenols	225:235	arg1	Blueberries					190:200	Blueberries	190:200	Blueberries	190:200	Blueberries are dietary sources of polyphenols with reported health benefits.
30471564	2	42	theme	polyphenols	225:235	arg1	sources					214:220	dietary sources	206:220	dietary sources of polyphenols with reported health benefits	206:265	Blueberries are dietary sources of polyphenols with reported health benefits.
30471564	10	43	theme	C57BL/6	1430:1436	arg1	mice					1440:1443	C57BL/6 J mice	1430:1443	C57BL/6 J mice	1430:1443	Our findings suggest that PPE, as a potential prebiotic agent, influences the gut microbiota to positively affect HFD-induced obesity in C57BL/6 J mice.
30471564	10	44	from	obesity	1419:1425	arg1	mice					1440:1443	C57BL/6 J mice	1430:1443	C57BL/6 J mice	1430:1443	Our findings suggest that PPE, as a potential prebiotic agent, influences the gut microbiota to positively affect HFD-induced obesity in C57BL/6 J mice.
30471564	3	45	theme	polyphenols	299:309	arg1	role					281:284	the role	277:284	the role of blueberry polyphenols in alleviating obesity	277:332	However, the role of blueberry polyphenols in alleviating obesity is not completely understood.
30471564	4	46	theme	high-fat	459:466	arg1	HFD					474:476	HFD	474:476	HFD	474:476	This study investigated the potential positive effect of blueberry polyphenol extract (PPE) on high-fat diet (HFD)-induced obesity in C57BL/6 J mice by modulation of the gut microbiota.
30471564	4	46	theme	high-fat	459:466	arg1	diet					468:471	high-fat diet	459:471	high-fat diet (HFD)	459:477	This study investigated the potential positive effect of blueberry polyphenol extract (PPE) on high-fat diet (HFD)-induced obesity in C57BL/6 J mice by modulation of the gut microbiota.
30471564	6	47	theme	tissue	716:721	arg1	weight					723:728	adipose tissue weight	708:728	adipose tissue weight	708:728	Mice fed HFD exhibited increased body weight and adipose tissue weight and disordered lipid metabolism.
30471564	8	48	from	composition	941:951	arg1	mice					988:991	C57BL/6 J mice	978:991	C57BL/6 J mice	978:991	Furthermore, 16S rRNA gene sequencing of the fecal microbiota suggested that PPE changed the composition of the gut microbiota in C57BL/6 J mice and modulated specific bacteria such as Proteobacteria, Deferribacteres, Actinobacteria, Bifidobacterium, Desulfovibrio, Adlercreutzia, Helicobacter, Flexispira, and Prevotella.
30471564	2	49	theme	dietary	206:212	arg1	Blueberries					190:200	Blueberries	190:200	Blueberries	190:200	Blueberries are dietary sources of polyphenols with reported health benefits.
30471564	2	49	theme	dietary	206:212	arg1	sources					214:220	dietary sources	206:220	dietary sources of polyphenols with reported health benefits	206:265	Blueberries are dietary sources of polyphenols with reported health benefits.
30471564	9	50	theme	HFD	1231:1233	arg1	mice					1235:1238	HFD mice	1231:1238	HFD mice	1231:1238	Orlistat also improved obesity and metabolic alterations of HFD mice and modulated the composition of the gut microbiota.
30471564	6	51	theme	adipose	708:714	arg1	weight					723:728	adipose tissue weight	708:728	adipose tissue weight	708:728	Mice fed HFD exhibited increased body weight and adipose tissue weight and disordered lipid metabolism.
30471564	9	52	theme	metabolic	1206:1214	arg1	alterations					1216:1226	metabolic alterations	1206:1226	metabolic alterations	1206:1226	Orlistat also improved obesity and metabolic alterations of HFD mice and modulated the composition of the gut microbiota.
30471564	8	53	theme	gene	870:873	arg1	sequencing					875:884	16S rRNA gene sequencing	861:884	16S rRNA gene sequencing of the fecal microbiota	861:908	Furthermore, 16S rRNA gene sequencing of the fecal microbiota suggested that PPE changed the composition of the gut microbiota in C57BL/6 J mice and modulated specific bacteria such as Proteobacteria, Deferribacteres, Actinobacteria, Bifidobacterium, Desulfovibrio, Adlercreutzia, Helicobacter, Flexispira, and Prevotella.
30471564	9	54	theme	microbiota	1281:1290	arg1	composition					1258:1268	the composition	1254:1268	the composition of the gut microbiota	1254:1290	Orlistat also improved obesity and metabolic alterations of HFD mice and modulated the composition of the gut microbiota.
30471564	4	55	theme	polyphenol	431:440	arg1	PPE					451:453	PPE	451:453	PPE	451:453	This study investigated the potential positive effect of blueberry polyphenol extract (PPE) on high-fat diet (HFD)-induced obesity in C57BL/6 J mice by modulation of the gut microbiota.
30471564	4	55	theme	polyphenol	431:440	arg1	extract					442:448	blueberry polyphenol extract	421:448	blueberry polyphenol extract (PPE)	421:454	This study investigated the potential positive effect of blueberry polyphenol extract (PPE) on high-fat diet (HFD)-induced obesity in C57BL/6 J mice by modulation of the gut microbiota.
30471564	5	56	dep	normal-fat	590:599	arg1	diet					601:604	diet	601:604	diet	601:604	Four-week-old C57BL/6 J mice were fed a normal-fat diet or HFD with or without PPE or Orlistat for 12 weeks.
30471564	3	57	theme	blueberry	289:297	arg1	polyphenols					299:309	blueberry polyphenols	289:309	blueberry polyphenols	289:309	However, the role of blueberry polyphenols in alleviating obesity is not completely understood.
30471564	9	58	theme	mice	1235:1238	arg1	obesity					1194:1200	obesity	1194:1200	obesity	1194:1200	Orlistat also improved obesity and metabolic alterations of HFD mice and modulated the composition of the gut microbiota.
30471564	9	58	theme	mice	1235:1238	arg1	alterations					1216:1226	metabolic alterations	1206:1226	metabolic alterations	1206:1226	Orlistat also improved obesity and metabolic alterations of HFD mice and modulated the composition of the gut microbiota.
30471564	8	59	theme	microbiota	899:908	arg1	sequencing					875:884	16S rRNA gene sequencing	861:884	16S rRNA gene sequencing of the fecal microbiota	861:908	Furthermore, 16S rRNA gene sequencing of the fecal microbiota suggested that PPE changed the composition of the gut microbiota in C57BL/6 J mice and modulated specific bacteria such as Proteobacteria, Deferribacteres, Actinobacteria, Bifidobacterium, Desulfovibrio, Adlercreutzia, Helicobacter, Flexispira, and Prevotella.
30471564	2	60	theme	health	251:256	arg1	benefits					258:265	reported health benefits	242:265	reported health benefits	242:265	Blueberries are dietary sources of polyphenols with reported health benefits.
30471564	0	61	with	prebiotic	45:53	arg1	effects					73:79	anti-obesity effects	60:79	anti-obesity effects on C57BL/6 J mice	60:97	Blueberry polyphenols extract as a potential prebiotic with anti-obesity effects on C57BL/6 J mice by modulating the gut microbiota.
30471564	10	62	theme	prebiotic	1339:1347	arg1	agent					1349:1353	a potential prebiotic agent	1327:1353	a potential prebiotic agent	1327:1353	Our findings suggest that PPE, as a potential prebiotic agent, influences the gut microbiota to positively affect HFD-induced obesity in C57BL/6 J mice.
30471564	10	62	theme	prebiotic	1339:1347	arg1	microbiota					1375:1384	the gut microbiota	1367:1384	the gut microbiota to positively affect HFD-induced obesity in C57BL/6 J mice	1367:1443	Our findings suggest that PPE, as a potential prebiotic agent, influences the gut microbiota to positively affect HFD-induced obesity in C57BL/6 J mice.
30471564	0	63	theme	anti-obesity	60:71	arg1	effects					73:79	anti-obesity effects	60:79	anti-obesity effects on C57BL/6 J mice	60:97	Blueberry polyphenols extract as a potential prebiotic with anti-obesity effects on C57BL/6 J mice by modulating the gut microbiota.
30471564	2	64	theme	reported	242:249	arg1	benefits					258:265	reported health benefits	242:265	reported health benefits	242:265	Blueberries are dietary sources of polyphenols with reported health benefits.
30471564	6	65	theme	increased	682:690	arg1	weight					697:702	increased body weight	682:702	increased body weight	682:702	Mice fed HFD exhibited increased body weight and adipose tissue weight and disordered lipid metabolism.
30165202	6	0	theme	chondrogenic	963:974	arg1	differentiation					976:990	maintained chondrogenic differentiation	952:990	maintained chondrogenic differentiation	952:990	In vitro assays demonstrated that AFF-MSCs possessed the typical MSC morphology and phenotype, and maintained chondrogenic differentiation properties when encapsulated within the hydrogel.
30165202	13	1	theme	accessible	1943:1952	arg1	approach					1954:1961	a simple and easy accessible approach	1925:1961	a simple and easy accessible approach to obtain a new stem cell source from arthroscopic flushing fluid (AFF-MSCs), which probably contains plenty of MSCs from synovium and synovial fluid	1925:2111	Hereby, we explored a simple and easy accessible approach to obtain a new stem cell source from arthroscopic flushing fluid (AFF-MSCs), which probably contains plenty of MSCs from synovium and synovial fluid.
30165202	11	2	theme	chondrogenic	1761:1772	arg1	potential					1774:1782	superior chondrogenic potential	1752:1782	superior chondrogenic potential	1752:1782	Recent studies determined that MSCs derived from synovium and synovial fluid exhibited superior chondrogenic potential.
30165202	3	3	theme	cartilage	502:510	arg1	regeneration					512:523	cartilage regeneration	502:523	cartilage regeneration	502:523	This study was designed to test the feasibility of using MSCs found in the human arthroscopic flushing fluid (AFF) for cartilage regeneration, by incorporating them into a newly developed one-step rapid cross-linking hyper-branched polyPEGDA/HA hydrogel.
30165202	1	4	theme	engineering	263:273	arg1	strategies					275:284	mesenchymal stem cell (MSC)-based tissue engineering strategies	222:284	mesenchymal stem cell (MSC)-based tissue engineering strategies	222:284	Many attempts have been made to repair articular cartilage defects, including mesenchymal stem cell (MSC)-based tissue engineering strategies.
30165202	12	5	theme	clinical	1883:1890	arg1	application					1892:1902	clinical application	1883:1902	clinical application	1883:1902	However, no feasible methods to harvest these human tissues and cells have been impeding them for clinical application.
30165202	4	6	theme	flushing	695:702	arg1	fluid					704:708	the original intra-articular flushing fluid	666:708	the original intra-articular flushing fluid of 10 patients prior to arthroscopic procedures	666:756	AFF-MSCs were isolated from the original intra-articular flushing fluid of 10 patients prior to arthroscopic procedures.
30165202	9	7	theme	mesenchymal	1510:1520	arg1	cell					1527:1530	mesenchymal stem cell	1510:1530	mesenchymal stem cell	1510:1530	STATEMENT OF SIGNIFICANCE Many attempts have been made to repair the defects of articular cartilage, including mesenchymal stem cell (MSC)-based tissue engineering strategies.
30165202	1	8	theme	cartilage	193:201	arg1	defects					203:209	articular cartilage defects	183:209	articular cartilage defects	183:209	Many attempts have been made to repair articular cartilage defects, including mesenchymal stem cell (MSC)-based tissue engineering strategies.
30165202	1	8	theme	cartilage	193:201	arg1	strategies					275:284	mesenchymal stem cell (MSC)-based tissue engineering strategies	222:284	mesenchymal stem cell (MSC)-based tissue engineering strategies	222:284	Many attempts have been made to repair articular cartilage defects, including mesenchymal stem cell (MSC)-based tissue engineering strategies.
30165202	8	9	theme	human	1287:1291	arg1	AFF-MSCs					1293:1300	human AFF-MSCs	1287:1300	human AFF-MSCs	1287:1300	These data suggest that human AFF-MSCs are a novel and abundant MSC source that have high therapeutic value for cartilage regeneration.
30165202	8	9	theme	human	1287:1291	arg1	source					1331:1336	a novel and abundant MSC source	1306:1336	a novel and abundant MSC source that have high therapeutic value for cartilage regeneration	1306:1396	These data suggest that human AFF-MSCs are a novel and abundant MSC source that have high therapeutic value for cartilage regeneration.
30165202	14	10	link	cross-linked	2206:2217	arg1	hydrogel					2232:2239	one-step rapid cross-linked polyPEGDA/HA hydrogel	2191:2239	one-step rapid cross-linked polyPEGDA/HA hydrogel	2191:2239	Further experiments demonstrated that encapsulation of these stem cells with one-step rapid cross-linked polyPEGDA/HA hydrogel held very encouraging potential for cartilage regeneration.
30165202	3	11	theme	one-step	571:578	arg1	hydrogel					628:635	a newly developed one-step rapid cross-linking hyper-branched polyPEGDA/HA hydrogel	553:635	a newly developed one-step rapid cross-linking hyper-branched polyPEGDA/HA hydrogel	553:635	This study was designed to test the feasibility of using MSCs found in the human arthroscopic flushing fluid (AFF) for cartilage regeneration, by incorporating them into a newly developed one-step rapid cross-linking hyper-branched polyPEGDA/HA hydrogel.
30165202	9	12	theme	tissue	1544:1549	arg1	strategies					1563:1572	mesenchymal stem cell (MSC)-based tissue engineering strategies	1510:1572	mesenchymal stem cell (MSC)-based tissue engineering strategies	1510:1572	STATEMENT OF SIGNIFICANCE Many attempts have been made to repair the defects of articular cartilage, including mesenchymal stem cell (MSC)-based tissue engineering strategies.
30165202	8	13	theme	therapeutic	1353:1363	arg1	value					1365:1369	high therapeutic value	1348:1369	high therapeutic value for cartilage regeneration	1348:1396	These data suggest that human AFF-MSCs are a novel and abundant MSC source that have high therapeutic value for cartilage regeneration.
30165202	1	14	theme	mesenchymal	222:232	arg1	cell					239:242	mesenchymal stem cell	222:242	mesenchymal stem cell	222:242	Many attempts have been made to repair articular cartilage defects, including mesenchymal stem cell (MSC)-based tissue engineering strategies.
30165202	8	15	theme	novel	1308:1312	arg1	AFF-MSCs					1293:1300	human AFF-MSCs	1287:1300	human AFF-MSCs	1287:1300	These data suggest that human AFF-MSCs are a novel and abundant MSC source that have high therapeutic value for cartilage regeneration.
30165202	8	15	theme	novel	1308:1312	arg1	source					1331:1336	a novel and abundant MSC source	1306:1336	a novel and abundant MSC source that have high therapeutic value for cartilage regeneration	1306:1396	These data suggest that human AFF-MSCs are a novel and abundant MSC source that have high therapeutic value for cartilage regeneration.
30165202	4	16	theme	arthroscopic	734:745	arg1	procedures					747:756	arthroscopic procedures	734:756	arthroscopic procedures	734:756	AFF-MSCs were isolated from the original intra-articular flushing fluid of 10 patients prior to arthroscopic procedures.
30165202	1	17	dep	-based	249:254	arg1	cell					239:242	mesenchymal stem cell	222:242	mesenchymal stem cell	222:242	Many attempts have been made to repair articular cartilage defects, including mesenchymal stem cell (MSC)-based tissue engineering strategies.
30165202	6	18	dep	In	853:854	arg1	vitro					856:860	vitro	856:860	vitro	856:860	In vitro assays demonstrated that AFF-MSCs possessed the typical MSC morphology and phenotype, and maintained chondrogenic differentiation properties when encapsulated within the hydrogel.
30165202	4	19	theme	original	670:677	arg1	fluid					704:708	the original intra-articular flushing fluid	666:708	the original intra-articular flushing fluid of 10 patients prior to arthroscopic procedures	666:756	AFF-MSCs were isolated from the original intra-articular flushing fluid of 10 patients prior to arthroscopic procedures.
30165202	9	20	theme	stem	1522:1525	arg1	cell					1527:1530	mesenchymal stem cell	1510:1530	mesenchymal stem cell	1510:1530	STATEMENT OF SIGNIFICANCE Many attempts have been made to repair the defects of articular cartilage, including mesenchymal stem cell (MSC)-based tissue engineering strategies.
30165202	3	21	theme	polyPEGDA/HA	615:626	arg1	hydrogel					628:635	a newly developed one-step rapid cross-linking hyper-branched polyPEGDA/HA hydrogel	553:635	a newly developed one-step rapid cross-linking hyper-branched polyPEGDA/HA hydrogel	553:635	This study was designed to test the feasibility of using MSCs found in the human arthroscopic flushing fluid (AFF) for cartilage regeneration, by incorporating them into a newly developed one-step rapid cross-linking hyper-branched polyPEGDA/HA hydrogel.
30165202	7	22	theme	cartilage	1115:1123	arg1	defects					1125:1131	full-thickness cartilage defects	1100:1131	full-thickness cartilage defects generated in a rat model	1100:1156	The AFF-MSC/hydrogel composite could significantly repair full-thickness cartilage defects generated in a rat model after 8 weeks of implantation; smooth cartilage was formed with evidence of hyaline cartilage formation.
30165202	8	23	theme	abundant	1318:1325	arg1	AFF-MSCs					1293:1300	human AFF-MSCs	1287:1300	human AFF-MSCs	1287:1300	These data suggest that human AFF-MSCs are a novel and abundant MSC source that have high therapeutic value for cartilage regeneration.
30165202	8	23	theme	abundant	1318:1325	arg1	source					1331:1336	a novel and abundant MSC source	1306:1336	a novel and abundant MSC source that have high therapeutic value for cartilage regeneration	1306:1396	These data suggest that human AFF-MSCs are a novel and abundant MSC source that have high therapeutic value for cartilage regeneration.
30165202	6	24	contain	possessed	896:904	arg2	differentiation					976:990	maintained chondrogenic differentiation	952:990	maintained chondrogenic differentiation	952:990	In vitro assays demonstrated that AFF-MSCs possessed the typical MSC morphology and phenotype, and maintained chondrogenic differentiation properties when encapsulated within the hydrogel.
30165202	6	24	contain	possessed	896:904	arg2	morphology					922:931	the typical MSC morphology	906:931	the typical MSC morphology	906:931	In vitro assays demonstrated that AFF-MSCs possessed the typical MSC morphology and phenotype, and maintained chondrogenic differentiation properties when encapsulated within the hydrogel.
30165202	6	24	contain	possessed	896:904	arg1	AFF-MSCs					887:894	AFF-MSCs	887:894	AFF-MSCs	887:894	In vitro assays demonstrated that AFF-MSCs possessed the typical MSC morphology and phenotype, and maintained chondrogenic differentiation properties when encapsulated within the hydrogel.
30165202	6	24	contain	possessed	896:904	arg2	phenotype					937:945	phenotype	937:945	phenotype	937:945	In vitro assays demonstrated that AFF-MSCs possessed the typical MSC morphology and phenotype, and maintained chondrogenic differentiation properties when encapsulated within the hydrogel.
30165202	14	25	theme	cartilage	2277:2285	arg1	regeneration					2287:2298	cartilage regeneration	2277:2298	cartilage regeneration	2277:2298	Further experiments demonstrated that encapsulation of these stem cells with one-step rapid cross-linked polyPEGDA/HA hydrogel held very encouraging potential for cartilage regeneration.
30165202	7	26	theme	rat	1148:1150	arg1	model					1152:1156	a rat model	1146:1156	a rat model	1146:1156	The AFF-MSC/hydrogel composite could significantly repair full-thickness cartilage defects generated in a rat model after 8 weeks of implantation; smooth cartilage was formed with evidence of hyaline cartilage formation.
30165202	3	27	theme	cross-linking	586:598	arg1	hydrogel					628:635	a newly developed one-step rapid cross-linking hyper-branched polyPEGDA/HA hydrogel	553:635	a newly developed one-step rapid cross-linking hyper-branched polyPEGDA/HA hydrogel	553:635	This study was designed to test the feasibility of using MSCs found in the human arthroscopic flushing fluid (AFF) for cartilage regeneration, by incorporating them into a newly developed one-step rapid cross-linking hyper-branched polyPEGDA/HA hydrogel.
30165202	11	28	theme	Recent	1665:1670	arg1	studies					1672:1678	Recent studies	1665:1678	Recent studies	1665:1678	Recent studies determined that MSCs derived from synovium and synovial fluid exhibited superior chondrogenic potential.
30165202	4	29	theme	patients	716:723	arg1	fluid					704:708	the original intra-articular flushing fluid	666:708	the original intra-articular flushing fluid of 10 patients prior to arthroscopic procedures	666:756	AFF-MSCs were isolated from the original intra-articular flushing fluid of 10 patients prior to arthroscopic procedures.
30165202	7	30	theme	AFF-MSC/hydrogel	1046:1061	arg1	composite					1063:1071	The AFF-MSC/hydrogel composite	1042:1071	The AFF-MSC/hydrogel composite	1042:1071	The AFF-MSC/hydrogel composite could significantly repair full-thickness cartilage defects generated in a rat model after 8 weeks of implantation; smooth cartilage was formed with evidence of hyaline cartilage formation.
30165202	13	31	from	synovium	2085:2092	arg1	plenty					2065:2070	plenty	2065:2070	plenty of MSCs from synovium and synovial fluid	2065:2111	Hereby, we explored a simple and easy accessible approach to obtain a new stem cell source from arthroscopic flushing fluid (AFF-MSCs), which probably contains plenty of MSCs from synovium and synovial fluid.
30165202	12	32	theme	harvest	1817:1823	arg1	tissues					1837:1843	these human tissues	1825:1843	these human tissues	1825:1843	However, no feasible methods to harvest these human tissues and cells have been impeding them for clinical application.
30165202	13	33	theme	arthroscopic	2001:2012	arg1	AFF-MSCs					2030:2037	AFF-MSCs	2030:2037	AFF-MSCs	2030:2037	Hereby, we explored a simple and easy accessible approach to obtain a new stem cell source from arthroscopic flushing fluid (AFF-MSCs), which probably contains plenty of MSCs from synovium and synovial fluid.
30165202	13	33	theme	arthroscopic	2001:2012	arg1	fluid					2023:2027	arthroscopic flushing fluid	2001:2027	arthroscopic flushing fluid (AFF-MSCs)	2001:2038	Hereby, we explored a simple and easy accessible approach to obtain a new stem cell source from arthroscopic flushing fluid (AFF-MSCs), which probably contains plenty of MSCs from synovium and synovial fluid.
30165202	5	34	theme	thiolated	821:829	arg1	HA					848:849	HA	848:849	HA	848:849	The hydrogel was fabricated with hyper-branched polyPEGDA and thiolated hyaluronic acid (HA).
30165202	5	34	theme	thiolated	821:829	arg1	acid					842:845	thiolated hyaluronic acid	821:845	thiolated hyaluronic acid (HA)	821:850	The hydrogel was fabricated with hyper-branched polyPEGDA and thiolated hyaluronic acid (HA).
30165202	12	35	theme	human	1831:1835	arg1	tissues					1837:1843	these human tissues	1825:1843	these human tissues	1825:1843	However, no feasible methods to harvest these human tissues and cells have been impeding them for clinical application.
30165202	14	36	theme	encouraging	2251:2261	arg1	potential					2263:2271	very encouraging potential	2246:2271	very encouraging potential	2246:2271	Further experiments demonstrated that encapsulation of these stem cells with one-step rapid cross-linked polyPEGDA/HA hydrogel held very encouraging potential for cartilage regeneration.
30165202	2	37	dep	challenging	370:380	arg1	optimizing					325:334	optimizing	325:334	optimizing MSC sources	325:346	Although this approach shows promise, optimizing MSC sources and their delivery is challenging.
30165202	0	38	theme	one-step	106:113	arg1	hydrogel					134:141	a one-step rapid cross-linked hydrogel	104:141	a one-step rapid cross-linked hydrogel	104:141	Cartilage regeneration using arthroscopic flushing fluid-derived mesenchymal stem cells encapsulated in a one-step rapid cross-linked hydrogel.
30165202	13	39	from	fluid	2023:2027	arg1	source					1989:1994	a new stem cell source	1973:1994	a new stem cell source	1973:1994	Hereby, we explored a simple and easy accessible approach to obtain a new stem cell source from arthroscopic flushing fluid (AFF-MSCs), which probably contains plenty of MSCs from synovium and synovial fluid.
30165202	10	40	theme	Optimizing	1575:1584	arg1	sources					1590:1596	Optimizing MSC sources	1575:1596	Optimizing MSC sources	1575:1596	Optimizing MSC sources and their delivery approaches still remain clinically challenging.
30165202	13	41	theme	stem	1979:1982	arg1	source					1989:1994	a new stem cell source	1973:1994	a new stem cell source	1973:1994	Hereby, we explored a simple and easy accessible approach to obtain a new stem cell source from arthroscopic flushing fluid (AFF-MSCs), which probably contains plenty of MSCs from synovium and synovial fluid.
30165202	0	42	theme	cross-linked	121:132	arg1	hydrogel					134:141	a one-step rapid cross-linked hydrogel	104:141	a one-step rapid cross-linked hydrogel	104:141	Cartilage regeneration using arthroscopic flushing fluid-derived mesenchymal stem cells encapsulated in a one-step rapid cross-linked hydrogel.
30165202	9	43	theme	Many	1425:1428	arg1	STATEMENT					1399:1407	STATEMENT	1399:1407	STATEMENT OF SIGNIFICANCE Many attempts	1399:1437	STATEMENT OF SIGNIFICANCE Many attempts have been made to repair the defects of articular cartilage, including mesenchymal stem cell (MSC)-based tissue engineering strategies.
30165202	5	44	theme	hyaluronic	831:840	arg1	HA					848:849	HA	848:849	HA	848:849	The hydrogel was fabricated with hyper-branched polyPEGDA and thiolated hyaluronic acid (HA).
30165202	5	44	theme	hyaluronic	831:840	arg1	acid					842:845	thiolated hyaluronic acid	821:845	thiolated hyaluronic acid (HA)	821:850	The hydrogel was fabricated with hyper-branched polyPEGDA and thiolated hyaluronic acid (HA).
30165202	14	45	theme	Further	2114:2120	arg1	experiments					2122:2132	Further experiments	2114:2132	Further experiments	2114:2132	Further experiments demonstrated that encapsulation of these stem cells with one-step rapid cross-linked polyPEGDA/HA hydrogel held very encouraging potential for cartilage regeneration.
30165202	14	46	theme	polyPEGDA/HA	2219:2230	arg1	hydrogel					2232:2239	one-step rapid cross-linked polyPEGDA/HA hydrogel	2191:2239	one-step rapid cross-linked polyPEGDA/HA hydrogel	2191:2239	Further experiments demonstrated that encapsulation of these stem cells with one-step rapid cross-linked polyPEGDA/HA hydrogel held very encouraging potential for cartilage regeneration.
30165202	3	47	theme	using	434:438	arg1	MSCs					440:443	using MSCs	434:443	using MSCs found in the human arthroscopic flushing fluid (AFF)	434:496	This study was designed to test the feasibility of using MSCs found in the human arthroscopic flushing fluid (AFF) for cartilage regeneration, by incorporating them into a newly developed one-step rapid cross-linking hyper-branched polyPEGDA/HA hydrogel.
30165202	0	48	theme	Cartilage	0:8	arg1	regeneration					10:21	Cartilage regeneration	0:21	Cartilage regeneration	0:21	Cartilage regeneration using arthroscopic flushing fluid-derived mesenchymal stem cells encapsulated in a one-step rapid cross-linked hydrogel.
30165202	14	49	theme	rapid	2200:2204	arg1	hydrogel					2232:2239	one-step rapid cross-linked polyPEGDA/HA hydrogel	2191:2239	one-step rapid cross-linked polyPEGDA/HA hydrogel	2191:2239	Further experiments demonstrated that encapsulation of these stem cells with one-step rapid cross-linked polyPEGDA/HA hydrogel held very encouraging potential for cartilage regeneration.
30165202	3	50	theme	arthroscopic	464:475	arg1	AFF					493:495	AFF	493:495	AFF	493:495	This study was designed to test the feasibility of using MSCs found in the human arthroscopic flushing fluid (AFF) for cartilage regeneration, by incorporating them into a newly developed one-step rapid cross-linking hyper-branched polyPEGDA/HA hydrogel.
30165202	3	50	theme	arthroscopic	464:475	arg1	fluid					486:490	the human arthroscopic flushing fluid	454:490	the human arthroscopic flushing fluid (AFF)	454:496	This study was designed to test the feasibility of using MSCs found in the human arthroscopic flushing fluid (AFF) for cartilage regeneration, by incorporating them into a newly developed one-step rapid cross-linking hyper-branched polyPEGDA/HA hydrogel.
30165202	6	51	theme	MSC	918:920	arg1	morphology					922:931	the typical MSC morphology	906:931	the typical MSC morphology	906:931	In vitro assays demonstrated that AFF-MSCs possessed the typical MSC morphology and phenotype, and maintained chondrogenic differentiation properties when encapsulated within the hydrogel.
30165202	9	52	dep	-based	1537:1542	arg1	cell					1527:1530	mesenchymal stem cell	1510:1530	mesenchymal stem cell	1510:1530	STATEMENT OF SIGNIFICANCE Many attempts have been made to repair the defects of articular cartilage, including mesenchymal stem cell (MSC)-based tissue engineering strategies.
30165202	0	53	theme	flushing	42:49	arg1	cells					82:86	arthroscopic flushing fluid-derived mesenchymal stem cells	29:86	arthroscopic flushing fluid-derived mesenchymal stem cells encapsulated in a one-step rapid cross-linked hydrogel	29:141	Cartilage regeneration using arthroscopic flushing fluid-derived mesenchymal stem cells encapsulated in a one-step rapid cross-linked hydrogel.
30165202	7	54	theme	formation	1252:1260	arg1	evidence					1222:1229	evidence	1222:1229	evidence of hyaline cartilage formation	1222:1260	The AFF-MSC/hydrogel composite could significantly repair full-thickness cartilage defects generated in a rat model after 8 weeks of implantation; smooth cartilage was formed with evidence of hyaline cartilage formation.
30165202	13	55	contain	contains	2056:2063	arg2	plenty					2065:2070	plenty	2065:2070	plenty of MSCs from synovium and synovial fluid	2065:2111	Hereby, we explored a simple and easy accessible approach to obtain a new stem cell source from arthroscopic flushing fluid (AFF-MSCs), which probably contains plenty of MSCs from synovium and synovial fluid.
30165202	13	55	contain	contains	2056:2063	arg1	source					1989:1994	a new stem cell source	1973:1994	a new stem cell source	1973:1994	Hereby, we explored a simple and easy accessible approach to obtain a new stem cell source from arthroscopic flushing fluid (AFF-MSCs), which probably contains plenty of MSCs from synovium and synovial fluid.
30165202	14	56	theme	stem	2175:2178	arg1	cells					2180:2184	these stem cells	2169:2184	these stem cells	2169:2184	Further experiments demonstrated that encapsulation of these stem cells with one-step rapid cross-linked polyPEGDA/HA hydrogel held very encouraging potential for cartilage regeneration.
30165202	0	57	theme	mesenchymal	65:75	arg1	cells					82:86	arthroscopic flushing fluid-derived mesenchymal stem cells	29:86	arthroscopic flushing fluid-derived mesenchymal stem cells encapsulated in a one-step rapid cross-linked hydrogel	29:141	Cartilage regeneration using arthroscopic flushing fluid-derived mesenchymal stem cells encapsulated in a one-step rapid cross-linked hydrogel.
30165202	9	58	theme	articular	1479:1487	arg1	cartilage					1489:1497	articular cartilage	1479:1497	articular cartilage	1479:1497	STATEMENT OF SIGNIFICANCE Many attempts have been made to repair the defects of articular cartilage, including mesenchymal stem cell (MSC)-based tissue engineering strategies.
30165202	6	59	dep	morphology	922:931	arg1	properties					992:1001	properties	992:1001	properties	992:1001	In vitro assays demonstrated that AFF-MSCs possessed the typical MSC morphology and phenotype, and maintained chondrogenic differentiation properties when encapsulated within the hydrogel.
30165202	13	60	theme	simple	1927:1932	arg1	approach					1954:1961	a simple and easy accessible approach	1925:1961	a simple and easy accessible approach to obtain a new stem cell source from arthroscopic flushing fluid (AFF-MSCs), which probably contains plenty of MSCs from synovium and synovial fluid	1925:2111	Hereby, we explored a simple and easy accessible approach to obtain a new stem cell source from arthroscopic flushing fluid (AFF-MSCs), which probably contains plenty of MSCs from synovium and synovial fluid.
30165202	11	61	theme	superior	1752:1759	arg1	potential					1774:1782	superior chondrogenic potential	1752:1782	superior chondrogenic potential	1752:1782	Recent studies determined that MSCs derived from synovium and synovial fluid exhibited superior chondrogenic potential.
30165202	8	62	theme	MSC	1327:1329	arg1	AFF-MSCs					1293:1300	human AFF-MSCs	1287:1300	human AFF-MSCs	1287:1300	These data suggest that human AFF-MSCs are a novel and abundant MSC source that have high therapeutic value for cartilage regeneration.
30165202	8	62	theme	MSC	1327:1329	arg1	source					1331:1336	a novel and abundant MSC source	1306:1336	a novel and abundant MSC source that have high therapeutic value for cartilage regeneration	1306:1396	These data suggest that human AFF-MSCs are a novel and abundant MSC source that have high therapeutic value for cartilage regeneration.
30165202	13	63	theme	easy	1938:1941	arg1	approach					1954:1961	a simple and easy accessible approach	1925:1961	a simple and easy accessible approach to obtain a new stem cell source from arthroscopic flushing fluid (AFF-MSCs), which probably contains plenty of MSCs from synovium and synovial fluid	1925:2111	Hereby, we explored a simple and easy accessible approach to obtain a new stem cell source from arthroscopic flushing fluid (AFF-MSCs), which probably contains plenty of MSCs from synovium and synovial fluid.
30165202	1	64	theme	tissue	256:261	arg1	strategies					275:284	mesenchymal stem cell (MSC)-based tissue engineering strategies	222:284	mesenchymal stem cell (MSC)-based tissue engineering strategies	222:284	Many attempts have been made to repair articular cartilage defects, including mesenchymal stem cell (MSC)-based tissue engineering strategies.
30165202	6	65	theme	maintained	952:961	arg1	differentiation					976:990	maintained chondrogenic differentiation	952:990	maintained chondrogenic differentiation	952:990	In vitro assays demonstrated that AFF-MSCs possessed the typical MSC morphology and phenotype, and maintained chondrogenic differentiation properties when encapsulated within the hydrogel.
30165202	6	66	theme	In	853:854	arg1	assays					862:867	In vitro assays	853:867	In vitro assays	853:867	In vitro assays demonstrated that AFF-MSCs possessed the typical MSC morphology and phenotype, and maintained chondrogenic differentiation properties when encapsulated within the hydrogel.
30165202	3	67	theme	developed	561:569	arg1	hydrogel					628:635	a newly developed one-step rapid cross-linking hyper-branched polyPEGDA/HA hydrogel	553:635	a newly developed one-step rapid cross-linking hyper-branched polyPEGDA/HA hydrogel	553:635	This study was designed to test the feasibility of using MSCs found in the human arthroscopic flushing fluid (AFF) for cartilage regeneration, by incorporating them into a newly developed one-step rapid cross-linking hyper-branched polyPEGDA/HA hydrogel.
30165202	11	68	attach	derived	1701:1707	arg2	MSCs					1696:1699	MSCs	1696:1699	MSCs derived from synovium and synovial fluid	1696:1740	Recent studies determined that MSCs derived from synovium and synovial fluid exhibited superior chondrogenic potential.
30165202	11	68	attach	derived	1701:1707	arg1	synovium					1714:1721	synovium	1714:1721	synovium	1714:1721	Recent studies determined that MSCs derived from synovium and synovial fluid exhibited superior chondrogenic potential.
30165202	11	68	attach	derived	1701:1707	arg1	fluid					1736:1740	synovial fluid	1727:1740	synovial fluid	1727:1740	Recent studies determined that MSCs derived from synovium and synovial fluid exhibited superior chondrogenic potential.
30165202	7	69	theme	smooth	1189:1194	arg1	cartilage					1196:1204	smooth cartilage	1189:1204	smooth cartilage	1189:1204	The AFF-MSC/hydrogel composite could significantly repair full-thickness cartilage defects generated in a rat model after 8 weeks of implantation; smooth cartilage was formed with evidence of hyaline cartilage formation.
30165202	1	70	theme	articular	183:191	arg1	defects					203:209	articular cartilage defects	183:209	articular cartilage defects	183:209	Many attempts have been made to repair articular cartilage defects, including mesenchymal stem cell (MSC)-based tissue engineering strategies.
30165202	1	70	theme	articular	183:191	arg1	strategies					275:284	mesenchymal stem cell (MSC)-based tissue engineering strategies	222:284	mesenchymal stem cell (MSC)-based tissue engineering strategies	222:284	Many attempts have been made to repair articular cartilage defects, including mesenchymal stem cell (MSC)-based tissue engineering strategies.
30165202	7	71	theme	hyaline	1234:1240	arg1	formation					1252:1260	hyaline cartilage formation	1234:1260	hyaline cartilage formation	1234:1260	The AFF-MSC/hydrogel composite could significantly repair full-thickness cartilage defects generated in a rat model after 8 weeks of implantation; smooth cartilage was formed with evidence of hyaline cartilage formation.
30165202	8	72	contain	have	1343:1346	arg1	AFF-MSCs					1293:1300	human AFF-MSCs	1287:1300	human AFF-MSCs	1287:1300	These data suggest that human AFF-MSCs are a novel and abundant MSC source that have high therapeutic value for cartilage regeneration.
30165202	8	72	contain	have	1343:1346	arg2	value					1365:1369	high therapeutic value	1348:1369	high therapeutic value for cartilage regeneration	1348:1396	These data suggest that human AFF-MSCs are a novel and abundant MSC source that have high therapeutic value for cartilage regeneration.
30165202	8	72	contain	have	1343:1346	arg1	source					1331:1336	a novel and abundant MSC source	1306:1336	a novel and abundant MSC source that have high therapeutic value for cartilage regeneration	1306:1396	These data suggest that human AFF-MSCs are a novel and abundant MSC source that have high therapeutic value for cartilage regeneration.
30165202	4	73	theme	intra-articular	679:693	arg1	fluid					704:708	the original intra-articular flushing fluid	666:708	the original intra-articular flushing fluid of 10 patients prior to arthroscopic procedures	666:756	AFF-MSCs were isolated from the original intra-articular flushing fluid of 10 patients prior to arthroscopic procedures.
30165202	7	74	theme	implantation	1175:1186	arg1	8 weeks					1164:1170	8 weeks	1164:1170	8 weeks of implantation	1164:1186	The AFF-MSC/hydrogel composite could significantly repair full-thickness cartilage defects generated in a rat model after 8 weeks of implantation; smooth cartilage was formed with evidence of hyaline cartilage formation.
30165202	14	75	with	encapsulation	2152:2164	arg1	hydrogel					2232:2239	one-step rapid cross-linked polyPEGDA/HA hydrogel	2191:2239	one-step rapid cross-linked polyPEGDA/HA hydrogel	2191:2239	Further experiments demonstrated that encapsulation of these stem cells with one-step rapid cross-linked polyPEGDA/HA hydrogel held very encouraging potential for cartilage regeneration.
30165202	2	76	theme	MSC	336:338	arg1	sources					340:346	MSC sources	336:346	MSC sources	336:346	Although this approach shows promise, optimizing MSC sources and their delivery is challenging.
30165202	9	77	theme	-based	1537:1542	arg1	strategies					1563:1572	mesenchymal stem cell (MSC)-based tissue engineering strategies	1510:1572	mesenchymal stem cell (MSC)-based tissue engineering strategies	1510:1572	STATEMENT OF SIGNIFICANCE Many attempts have been made to repair the defects of articular cartilage, including mesenchymal stem cell (MSC)-based tissue engineering strategies.
30165202	0	78	link	fluid-derived	51:63	arg1	cells					82:86	arthroscopic flushing fluid-derived mesenchymal stem cells	29:86	arthroscopic flushing fluid-derived mesenchymal stem cells encapsulated in a one-step rapid cross-linked hydrogel	29:141	Cartilage regeneration using arthroscopic flushing fluid-derived mesenchymal stem cells encapsulated in a one-step rapid cross-linked hydrogel.
30165202	14	79	theme	cells	2180:2184	arg1	encapsulation					2152:2164	encapsulation	2152:2164	encapsulation of these stem cells with one-step rapid cross-linked polyPEGDA/HA hydrogel	2152:2239	Further experiments demonstrated that encapsulation of these stem cells with one-step rapid cross-linked polyPEGDA/HA hydrogel held very encouraging potential for cartilage regeneration.
30165202	9	80	theme	engineering	1551:1561	arg1	strategies					1563:1572	mesenchymal stem cell (MSC)-based tissue engineering strategies	1510:1572	mesenchymal stem cell (MSC)-based tissue engineering strategies	1510:1572	STATEMENT OF SIGNIFICANCE Many attempts have been made to repair the defects of articular cartilage, including mesenchymal stem cell (MSC)-based tissue engineering strategies.
30165202	8	81	theme	high	1348:1351	arg1	value					1365:1369	high therapeutic value	1348:1369	high therapeutic value for cartilage regeneration	1348:1396	These data suggest that human AFF-MSCs are a novel and abundant MSC source that have high therapeutic value for cartilage regeneration.
30165202	3	82	theme	hyper-branched	600:613	arg1	hydrogel					628:635	a newly developed one-step rapid cross-linking hyper-branched polyPEGDA/HA hydrogel	553:635	a newly developed one-step rapid cross-linking hyper-branched polyPEGDA/HA hydrogel	553:635	This study was designed to test the feasibility of using MSCs found in the human arthroscopic flushing fluid (AFF) for cartilage regeneration, by incorporating them into a newly developed one-step rapid cross-linking hyper-branched polyPEGDA/HA hydrogel.
30165202	1	83	theme	stem	234:237	arg1	cell					239:242	mesenchymal stem cell	222:242	mesenchymal stem cell	222:242	Many attempts have been made to repair articular cartilage defects, including mesenchymal stem cell (MSC)-based tissue engineering strategies.
30165202	3	84	theme	rapid	580:584	arg1	hydrogel					628:635	a newly developed one-step rapid cross-linking hyper-branched polyPEGDA/HA hydrogel	553:635	a newly developed one-step rapid cross-linking hyper-branched polyPEGDA/HA hydrogel	553:635	This study was designed to test the feasibility of using MSCs found in the human arthroscopic flushing fluid (AFF) for cartilage regeneration, by incorporating them into a newly developed one-step rapid cross-linking hyper-branched polyPEGDA/HA hydrogel.
30165202	8	85	theme	cartilage	1375:1383	arg1	regeneration					1385:1396	cartilage regeneration	1375:1396	cartilage regeneration	1375:1396	These data suggest that human AFF-MSCs are a novel and abundant MSC source that have high therapeutic value for cartilage regeneration.
30165202	7	86	theme	full-thickness	1100:1113	arg1	defects					1125:1131	full-thickness cartilage defects	1100:1131	full-thickness cartilage defects generated in a rat model	1100:1156	The AFF-MSC/hydrogel composite could significantly repair full-thickness cartilage defects generated in a rat model after 8 weeks of implantation; smooth cartilage was formed with evidence of hyaline cartilage formation.
30165202	0	87	theme	fluid-derived	51:63	arg1	cells					82:86	arthroscopic flushing fluid-derived mesenchymal stem cells	29:86	arthroscopic flushing fluid-derived mesenchymal stem cells encapsulated in a one-step rapid cross-linked hydrogel	29:141	Cartilage regeneration using arthroscopic flushing fluid-derived mesenchymal stem cells encapsulated in a one-step rapid cross-linked hydrogel.
30165202	5	88	theme	hyper-branched	792:805	arg1	polyPEGDA					807:815	hyper-branched polyPEGDA	792:815	hyper-branched polyPEGDA	792:815	The hydrogel was fabricated with hyper-branched polyPEGDA and thiolated hyaluronic acid (HA).
30165202	13	89	from	fluid	2107:2111	arg1	plenty					2065:2070	plenty	2065:2070	plenty of MSCs from synovium and synovial fluid	2065:2111	Hereby, we explored a simple and easy accessible approach to obtain a new stem cell source from arthroscopic flushing fluid (AFF-MSCs), which probably contains plenty of MSCs from synovium and synovial fluid.
30165202	4	90	theme	prior	725:729	arg1	patients					716:723	10 patients	713:723	10 patients prior to arthroscopic procedures	713:756	AFF-MSCs were isolated from the original intra-articular flushing fluid of 10 patients prior to arthroscopic procedures.
30165202	13	91	theme	flushing	2014:2021	arg1	AFF-MSCs					2030:2037	AFF-MSCs	2030:2037	AFF-MSCs	2030:2037	Hereby, we explored a simple and easy accessible approach to obtain a new stem cell source from arthroscopic flushing fluid (AFF-MSCs), which probably contains plenty of MSCs from synovium and synovial fluid.
30165202	13	91	theme	flushing	2014:2021	arg1	fluid					2023:2027	arthroscopic flushing fluid	2001:2027	arthroscopic flushing fluid (AFF-MSCs)	2001:2038	Hereby, we explored a simple and easy accessible approach to obtain a new stem cell source from arthroscopic flushing fluid (AFF-MSCs), which probably contains plenty of MSCs from synovium and synovial fluid.
30165202	0	92	theme	rapid	115:119	arg1	hydrogel					134:141	a one-step rapid cross-linked hydrogel	104:141	a one-step rapid cross-linked hydrogel	104:141	Cartilage regeneration using arthroscopic flushing fluid-derived mesenchymal stem cells encapsulated in a one-step rapid cross-linked hydrogel.
30165202	13	93	theme	new	1975:1977	arg1	source					1989:1994	a new stem cell source	1973:1994	a new stem cell source	1973:1994	Hereby, we explored a simple and easy accessible approach to obtain a new stem cell source from arthroscopic flushing fluid (AFF-MSCs), which probably contains plenty of MSCs from synovium and synovial fluid.
30165202	1	94	dep	Many	144:147	arg1	attempts					149:156	attempts	149:156	attempts	149:156	Many attempts have been made to repair articular cartilage defects, including mesenchymal stem cell (MSC)-based tissue engineering strategies.
30165202	13	95	theme	cell	1984:1987	arg1	source					1989:1994	a new stem cell source	1973:1994	a new stem cell source	1973:1994	Hereby, we explored a simple and easy accessible approach to obtain a new stem cell source from arthroscopic flushing fluid (AFF-MSCs), which probably contains plenty of MSCs from synovium and synovial fluid.
30165202	13	96	theme	MSCs	2075:2078	arg1	plenty					2065:2070	plenty	2065:2070	plenty of MSCs from synovium and synovial fluid	2065:2111	Hereby, we explored a simple and easy accessible approach to obtain a new stem cell source from arthroscopic flushing fluid (AFF-MSCs), which probably contains plenty of MSCs from synovium and synovial fluid.
30165202	3	97	located	found	445:449	arg1	AFF					493:495	AFF	493:495	AFF	493:495	This study was designed to test the feasibility of using MSCs found in the human arthroscopic flushing fluid (AFF) for cartilage regeneration, by incorporating them into a newly developed one-step rapid cross-linking hyper-branched polyPEGDA/HA hydrogel.
30165202	3	97	located	found	445:449	arg1	fluid					486:490	the human arthroscopic flushing fluid	454:490	the human arthroscopic flushing fluid (AFF)	454:496	This study was designed to test the feasibility of using MSCs found in the human arthroscopic flushing fluid (AFF) for cartilage regeneration, by incorporating them into a newly developed one-step rapid cross-linking hyper-branched polyPEGDA/HA hydrogel.
30165202	3	97	located	found	445:449	arg2	MSCs					440:443	using MSCs	434:443	using MSCs found in the human arthroscopic flushing fluid (AFF)	434:496	This study was designed to test the feasibility of using MSCs found in the human arthroscopic flushing fluid (AFF) for cartilage regeneration, by incorporating them into a newly developed one-step rapid cross-linking hyper-branched polyPEGDA/HA hydrogel.
30165202	12	98	theme	feasible	1797:1804	arg1	methods					1806:1812	no feasible methods	1794:1812	no feasible methods to harvest these human tissues and cells	1794:1853	However, no feasible methods to harvest these human tissues and cells have been impeding them for clinical application.
30165202	14	99	theme	cross-linked	2206:2217	arg1	hydrogel					2232:2239	one-step rapid cross-linked polyPEGDA/HA hydrogel	2191:2239	one-step rapid cross-linked polyPEGDA/HA hydrogel	2191:2239	Further experiments demonstrated that encapsulation of these stem cells with one-step rapid cross-linked polyPEGDA/HA hydrogel held very encouraging potential for cartilage regeneration.
30165202	3	100	theme	MSCs	440:443	arg1	feasibility					419:429	the feasibility	415:429	the feasibility of using MSCs found in the human arthroscopic flushing fluid (AFF)	415:496	This study was designed to test the feasibility of using MSCs found in the human arthroscopic flushing fluid (AFF) for cartilage regeneration, by incorporating them into a newly developed one-step rapid cross-linking hyper-branched polyPEGDA/HA hydrogel.
30165202	0	101	theme	arthroscopic	29:40	arg1	cells					82:86	arthroscopic flushing fluid-derived mesenchymal stem cells	29:86	arthroscopic flushing fluid-derived mesenchymal stem cells encapsulated in a one-step rapid cross-linked hydrogel	29:141	Cartilage regeneration using arthroscopic flushing fluid-derived mesenchymal stem cells encapsulated in a one-step rapid cross-linked hydrogel.
30165202	13	102	theme	synovial	2098:2105	arg1	fluid					2107:2111	synovial fluid	2098:2111	synovial fluid	2098:2111	Hereby, we explored a simple and easy accessible approach to obtain a new stem cell source from arthroscopic flushing fluid (AFF-MSCs), which probably contains plenty of MSCs from synovium and synovial fluid.
30165202	14	103	theme	one-step	2191:2198	arg1	hydrogel					2232:2239	one-step rapid cross-linked polyPEGDA/HA hydrogel	2191:2239	one-step rapid cross-linked polyPEGDA/HA hydrogel	2191:2239	Further experiments demonstrated that encapsulation of these stem cells with one-step rapid cross-linked polyPEGDA/HA hydrogel held very encouraging potential for cartilage regeneration.
30165202	3	104	theme	flushing	477:484	arg1	AFF					493:495	AFF	493:495	AFF	493:495	This study was designed to test the feasibility of using MSCs found in the human arthroscopic flushing fluid (AFF) for cartilage regeneration, by incorporating them into a newly developed one-step rapid cross-linking hyper-branched polyPEGDA/HA hydrogel.
30165202	3	104	theme	flushing	477:484	arg1	fluid					486:490	the human arthroscopic flushing fluid	454:490	the human arthroscopic flushing fluid (AFF)	454:496	This study was designed to test the feasibility of using MSCs found in the human arthroscopic flushing fluid (AFF) for cartilage regeneration, by incorporating them into a newly developed one-step rapid cross-linking hyper-branched polyPEGDA/HA hydrogel.
30165202	7	105	theme	cartilage	1242:1250	arg1	formation					1252:1260	hyaline cartilage formation	1234:1260	hyaline cartilage formation	1234:1260	The AFF-MSC/hydrogel composite could significantly repair full-thickness cartilage defects generated in a rat model after 8 weeks of implantation; smooth cartilage was formed with evidence of hyaline cartilage formation.
30165202	6	106	theme	typical	910:916	arg1	morphology					922:931	the typical MSC morphology	906:931	the typical MSC morphology	906:931	In vitro assays demonstrated that AFF-MSCs possessed the typical MSC morphology and phenotype, and maintained chondrogenic differentiation properties when encapsulated within the hydrogel.
30165202	9	107	dep	Many	1425:1428	arg1	attempts					1430:1437	attempts	1430:1437	attempts	1430:1437	STATEMENT OF SIGNIFICANCE Many attempts have been made to repair the defects of articular cartilage, including mesenchymal stem cell (MSC)-based tissue engineering strategies.
30165202	10	108	theme	MSC	1586:1588	arg1	sources					1590:1596	Optimizing MSC sources	1575:1596	Optimizing MSC sources	1575:1596	Optimizing MSC sources and their delivery approaches still remain clinically challenging.
30165202	3	109	theme	human	458:462	arg1	AFF					493:495	AFF	493:495	AFF	493:495	This study was designed to test the feasibility of using MSCs found in the human arthroscopic flushing fluid (AFF) for cartilage regeneration, by incorporating them into a newly developed one-step rapid cross-linking hyper-branched polyPEGDA/HA hydrogel.
30165202	3	109	theme	human	458:462	arg1	fluid					486:490	the human arthroscopic flushing fluid	454:490	the human arthroscopic flushing fluid (AFF)	454:496	This study was designed to test the feasibility of using MSCs found in the human arthroscopic flushing fluid (AFF) for cartilage regeneration, by incorporating them into a newly developed one-step rapid cross-linking hyper-branched polyPEGDA/HA hydrogel.
30165202	0	110	theme	stem	77:80	arg1	cells					82:86	arthroscopic flushing fluid-derived mesenchymal stem cells	29:86	arthroscopic flushing fluid-derived mesenchymal stem cells encapsulated in a one-step rapid cross-linked hydrogel	29:141	Cartilage regeneration using arthroscopic flushing fluid-derived mesenchymal stem cells encapsulated in a one-step rapid cross-linked hydrogel.
30165202	4	111	attach	isolated	652:659	arg2	AFF-MSCs					638:645	AFF-MSCs	638:645	AFF-MSCs	638:645	AFF-MSCs were isolated from the original intra-articular flushing fluid of 10 patients prior to arthroscopic procedures.
30165202	4	111	attach	isolated	652:659	arg1	fluid					704:708	the original intra-articular flushing fluid	666:708	the original intra-articular flushing fluid of 10 patients prior to arthroscopic procedures	666:756	AFF-MSCs were isolated from the original intra-articular flushing fluid of 10 patients prior to arthroscopic procedures.
30165202	10	112	theme	delivery	1608:1615	arg1	approaches					1617:1626	their delivery approaches	1602:1626	their delivery approaches	1602:1626	Optimizing MSC sources and their delivery approaches still remain clinically challenging.
30165202	9	113	theme	cartilage	1489:1497	arg1	strategies					1563:1572	mesenchymal stem cell (MSC)-based tissue engineering strategies	1510:1572	mesenchymal stem cell (MSC)-based tissue engineering strategies	1510:1572	STATEMENT OF SIGNIFICANCE Many attempts have been made to repair the defects of articular cartilage, including mesenchymal stem cell (MSC)-based tissue engineering strategies.
30165202	9	113	theme	cartilage	1489:1497	arg1	defects					1468:1474	the defects	1464:1474	the defects	1464:1474	STATEMENT OF SIGNIFICANCE Many attempts have been made to repair the defects of articular cartilage, including mesenchymal stem cell (MSC)-based tissue engineering strategies.
30165202	0	114	link	cross-linked	121:132	arg1	hydrogel					134:141	a one-step rapid cross-linked hydrogel	104:141	a one-step rapid cross-linked hydrogel	104:141	Cartilage regeneration using arthroscopic flushing fluid-derived mesenchymal stem cells encapsulated in a one-step rapid cross-linked hydrogel.
30165202	11	115	theme	synovial	1727:1734	arg1	fluid					1736:1740	synovial fluid	1727:1740	synovial fluid	1727:1740	Recent studies determined that MSCs derived from synovium and synovial fluid exhibited superior chondrogenic potential.
30165202	1	116	theme	-based	249:254	arg1	strategies					275:284	mesenchymal stem cell (MSC)-based tissue engineering strategies	222:284	mesenchymal stem cell (MSC)-based tissue engineering strategies	222:284	Many attempts have been made to repair articular cartilage defects, including mesenchymal stem cell (MSC)-based tissue engineering strategies.
30744123	5	0	dep	able	1051:1054	arg1	Using					956:960	Using	956:960	Using gas chromatography and liquid chromatography tandem mass spectrometry (LC-MS/MS)	956:1041	Using gas chromatography and liquid chromatography tandem mass spectrometry (LC-MS/MS) we were able to analyze fatty acid composition and the formation of lipid mediators called oxylipins.
30744123	2	1	theme	essential	356:364	arg1	acids					408:412	polyunsaturated fatty acids	386:412	polyunsaturated fatty acids (n-6 and n-3 PUFAs)	386:432	Besides lowering lipoproteins and triglycerides, apheresis also decreases levels of essential omega-6 and omega-3 polyunsaturated fatty acids (n-6 and n-3 PUFAs) in blood plasma.
30744123	2	1	theme	essential	356:364	arg1	PUFAs					427:431	n-6 and n-3 PUFAs	415:431	PUFAs	427:431	Besides lowering lipoproteins and triglycerides, apheresis also decreases levels of essential omega-6 and omega-3 polyunsaturated fatty acids (n-6 and n-3 PUFAs) in blood plasma.
30744123	6	2	theme	significant	1306:1316	arg1	increases					1318:1326	significant increases	1306:1326	significant increases of PUFA-derived lipoxygenase-, as well as cyclooxygenase- and cytochrome P450-derived lipid mediators	1306:1428	Our data illustrate-particularly in HELP-treated patients-significant decreases of essential omega-6 and omega-3 polyunsaturated fatty acids in blood plasma but significant increases of PUFA-derived lipoxygenase-, as well as cyclooxygenase- and cytochrome P450-derived lipid mediators.
30744123	2	3	theme	lowering	280:287	arg1	lipoproteins					289:300	lowering lipoproteins	280:300	lowering lipoproteins	280:300	Besides lowering lipoproteins and triglycerides, apheresis also decreases levels of essential omega-6 and omega-3 polyunsaturated fatty acids (n-6 and n-3 PUFAs) in blood plasma.
30744123	6	4	from	plasma	1295:1300	arg1	decreases					1215:1223	HELP-treated patients-significant decreases	1181:1223	HELP-treated patients-significant decreases of essential omega-6 and omega-3 polyunsaturated fatty acids in blood plasma	1181:1300	Our data illustrate-particularly in HELP-treated patients-significant decreases of essential omega-6 and omega-3 polyunsaturated fatty acids in blood plasma but significant increases of PUFA-derived lipoxygenase-, as well as cyclooxygenase- and cytochrome P450-derived lipid mediators.
30744123	2	5	from	levels	346:351	arg1	plasma					443:448	blood plasma	437:448	blood plasma	437:448	Besides lowering lipoproteins and triglycerides, apheresis also decreases levels of essential omega-6 and omega-3 polyunsaturated fatty acids (n-6 and n-3 PUFAs) in blood plasma.
30744123	6	6	theme	HELP-treated	1181:1192	arg1	decreases					1215:1223	HELP-treated patients-significant decreases	1181:1223	HELP-treated patients-significant decreases of essential omega-6 and omega-3 polyunsaturated fatty acids in blood plasma	1181:1300	Our data illustrate-particularly in HELP-treated patients-significant decreases of essential omega-6 and omega-3 polyunsaturated fatty acids in blood plasma but significant increases of PUFA-derived lipoxygenase-, as well as cyclooxygenase- and cytochrome P450-derived lipid mediators.
30744123	7	7	link	PUFA-derived	1510:1521	arg1	mediators					1529:1537	n-3 PUFA-derived lipid mediators	1506:1537	n-3 PUFA-derived lipid mediators	1506:1537	Given that n-3 PUFAs in particular are presumed to be cardioprotective and n-3 PUFA-derived lipid mediators might limit inflammatory reactions, these data indicate that n-3 PUFA supplementation in the context of lipid apheresis treatment might have additional benefits through apheresis-triggered protective n-3 PUFA-derived lipid mediators.
30744123	7	8	theme	PUFA-derived	1510:1521	arg1	mediators					1529:1537	n-3 PUFA-derived lipid mediators	1506:1537	n-3 PUFA-derived lipid mediators	1506:1537	Given that n-3 PUFAs in particular are presumed to be cardioprotective and n-3 PUFA-derived lipid mediators might limit inflammatory reactions, these data indicate that n-3 PUFA supplementation in the context of lipid apheresis treatment might have additional benefits through apheresis-triggered protective n-3 PUFA-derived lipid mediators.
30744123	7	9	theme	PUFA-derived	1743:1754	arg1	mediators					1762:1770	apheresis-triggered protective n-3 PUFA-derived lipid mediators	1708:1770	apheresis-triggered protective n-3 PUFA-derived lipid mediators	1708:1770	Given that n-3 PUFAs in particular are presumed to be cardioprotective and n-3 PUFA-derived lipid mediators might limit inflammatory reactions, these data indicate that n-3 PUFA supplementation in the context of lipid apheresis treatment might have additional benefits through apheresis-triggered protective n-3 PUFA-derived lipid mediators.
30744123	1	10	theme	elevated	238:245	arg1	a					260:260	a	260:260	a	260:260	Lipoprotein apheresis reliably reduces low-density lipoprotein (LDL) cholesterol in patients with atherosclerotic disease and therapy-refractory hypercholesterolemia or elevated lipoprotein (a) (Lp(a)).
30744123	1	10	theme	elevated	238:245	arg1	lipoprotein					247:257	elevated lipoprotein	238:257	elevated lipoprotein (a) (Lp(a))	238:269	Lipoprotein apheresis reliably reduces low-density lipoprotein (LDL) cholesterol in patients with atherosclerotic disease and therapy-refractory hypercholesterolemia or elevated lipoprotein (a) (Lp(a)).
30744123	7	11	theme	protective	1728:1737	arg1	mediators					1762:1770	apheresis-triggered protective n-3 PUFA-derived lipid mediators	1708:1770	apheresis-triggered protective n-3 PUFA-derived lipid mediators	1708:1770	Given that n-3 PUFAs in particular are presumed to be cardioprotective and n-3 PUFA-derived lipid mediators might limit inflammatory reactions, these data indicate that n-3 PUFA supplementation in the context of lipid apheresis treatment might have additional benefits through apheresis-triggered protective n-3 PUFA-derived lipid mediators.
30744123	4	12	theme	pre-	920:923	arg1	comparison					944:953	a direct pre- and post-apheresis comparison	911:953	a direct pre- and post-apheresis comparison	911:953	The study presented here analyzed lipid mediator profiles in the plasma of patients with hyperlipidemia treated by one of three different apheresis methods, either HELP, direct absorption (DA), or membrane filtration (MDF), in a direct pre- and post-apheresis comparison.
30744123	6	13	theme	acids	1280:1284	arg1	decreases					1215:1223	HELP-treated patients-significant decreases	1181:1223	HELP-treated patients-significant decreases of essential omega-6 and omega-3 polyunsaturated fatty acids in blood plasma	1181:1300	Our data illustrate-particularly in HELP-treated patients-significant decreases of essential omega-6 and omega-3 polyunsaturated fatty acids in blood plasma but significant increases of PUFA-derived lipoxygenase-, as well as cyclooxygenase- and cytochrome P450-derived lipid mediators.
30744123	5	14	theme	acid	1073:1076	arg1	composition					1078:1088	fatty acid composition	1067:1088	fatty acid composition	1067:1088	Using gas chromatography and liquid chromatography tandem mass spectrometry (LC-MS/MS) we were able to analyze fatty acid composition and the formation of lipid mediators called oxylipins.
30744123	3	15	theme	lipid	636:640	arg1	mediators					642:650	potentially pro-inflammatory and pro-thrombotic lipid mediators	588:650	potentially pro-inflammatory and pro-thrombotic lipid mediators derived from n-6 and n-3 PUFAs	588:681	In contrast, heparin-induced extracorporeal low-density lipoprotein precipitation (HELP) lipid apheresis might increase the formation of potentially pro-inflammatory and pro-thrombotic lipid mediators derived from n-6 and n-3 PUFAs.
30744123	6	16	theme	polyunsaturated	1258:1272	arg1	acids					1280:1284	essential omega-6 and omega-3 polyunsaturated fatty acids	1228:1284	acids	1280:1284	Our data illustrate-particularly in HELP-treated patients-significant decreases of essential omega-6 and omega-3 polyunsaturated fatty acids in blood plasma but significant increases of PUFA-derived lipoxygenase-, as well as cyclooxygenase- and cytochrome P450-derived lipid mediators.
30744123	7	17	theme	additional	1680:1689	arg1	benefits					1691:1698	additional benefits	1680:1698	additional benefits	1680:1698	Given that n-3 PUFAs in particular are presumed to be cardioprotective and n-3 PUFA-derived lipid mediators might limit inflammatory reactions, these data indicate that n-3 PUFA supplementation in the context of lipid apheresis treatment might have additional benefits through apheresis-triggered protective n-3 PUFA-derived lipid mediators.
30744123	6	18	theme	lipoxygenase-	1344:1356	arg1	mediators					1420:1428	PUFA-derived lipoxygenase-, as well as cyclooxygenase- and cytochrome P450-derived lipid mediators	1331:1428	PUFA-derived lipoxygenase-, as well as cyclooxygenase- and cytochrome P450-derived lipid mediators	1331:1428	Our data illustrate-particularly in HELP-treated patients-significant decreases of essential omega-6 and omega-3 polyunsaturated fatty acids in blood plasma but significant increases of PUFA-derived lipoxygenase-, as well as cyclooxygenase- and cytochrome P450-derived lipid mediators.
30744123	3	19	theme	n-6	665:667	arg1	PUFAs					677:681	n-6 and n-3 PUFAs	665:681	PUFAs	677:681	In contrast, heparin-induced extracorporeal low-density lipoprotein precipitation (HELP) lipid apheresis might increase the formation of potentially pro-inflammatory and pro-thrombotic lipid mediators derived from n-6 and n-3 PUFAs.
30744123	3	20	attach	derived	652:658	arg1	PUFAs					677:681	n-6 and n-3 PUFAs	665:681	PUFAs	677:681	In contrast, heparin-induced extracorporeal low-density lipoprotein precipitation (HELP) lipid apheresis might increase the formation of potentially pro-inflammatory and pro-thrombotic lipid mediators derived from n-6 and n-3 PUFAs.
30744123	3	20	attach	derived	652:658	arg2	mediators					642:650	potentially pro-inflammatory and pro-thrombotic lipid mediators	588:650	potentially pro-inflammatory and pro-thrombotic lipid mediators derived from n-6 and n-3 PUFAs	588:681	In contrast, heparin-induced extracorporeal low-density lipoprotein precipitation (HELP) lipid apheresis might increase the formation of potentially pro-inflammatory and pro-thrombotic lipid mediators derived from n-6 and n-3 PUFAs.
30744123	4	21	theme	lipid	718:722	arg1	profiles					733:740	lipid mediator profiles	718:740	lipid mediator profiles in the plasma of patients with hyperlipidemia treated by one of three different apheresis methods, either HELP, direct absorption (DA), or membrane filtration (MDF)	718:905	The study presented here analyzed lipid mediator profiles in the plasma of patients with hyperlipidemia treated by one of three different apheresis methods, either HELP, direct absorption (DA), or membrane filtration (MDF), in a direct pre- and post-apheresis comparison.
30744123	5	22	theme	lipid	1111:1115	arg1	mediators					1117:1125	lipid mediators	1111:1125	lipid mediators called oxylipins	1111:1142	Using gas chromatography and liquid chromatography tandem mass spectrometry (LC-MS/MS) we were able to analyze fatty acid composition and the formation of lipid mediators called oxylipins.
30744123	7	23	theme	apheresis	1649:1657	arg1	treatment					1659:1667	lipid apheresis treatment	1643:1667	lipid apheresis treatment	1643:1667	Given that n-3 PUFAs in particular are presumed to be cardioprotective and n-3 PUFA-derived lipid mediators might limit inflammatory reactions, these data indicate that n-3 PUFA supplementation in the context of lipid apheresis treatment might have additional benefits through apheresis-triggered protective n-3 PUFA-derived lipid mediators.
30744123	7	24	link	PUFA-derived	1743:1754	arg1	mediators					1762:1770	apheresis-triggered protective n-3 PUFA-derived lipid mediators	1708:1770	apheresis-triggered protective n-3 PUFA-derived lipid mediators	1708:1770	Given that n-3 PUFAs in particular are presumed to be cardioprotective and n-3 PUFA-derived lipid mediators might limit inflammatory reactions, these data indicate that n-3 PUFA supplementation in the context of lipid apheresis treatment might have additional benefits through apheresis-triggered protective n-3 PUFA-derived lipid mediators.
30744123	1	25	theme	atherosclerotic	167:181	arg1	disease					183:189	atherosclerotic disease	167:189	atherosclerotic disease	167:189	Lipoprotein apheresis reliably reduces low-density lipoprotein (LDL) cholesterol in patients with atherosclerotic disease and therapy-refractory hypercholesterolemia or elevated lipoprotein (a) (Lp(a)).
30744123	4	26	theme	post-apheresis	929:942	arg1	comparison					944:953	a direct pre- and post-apheresis comparison	911:953	a direct pre- and post-apheresis comparison	911:953	The study presented here analyzed lipid mediator profiles in the plasma of patients with hyperlipidemia treated by one of three different apheresis methods, either HELP, direct absorption (DA), or membrane filtration (MDF), in a direct pre- and post-apheresis comparison.
30744123	6	27	from	omega-6	1238:1244	arg1	plasma					1295:1300	blood plasma	1289:1300	blood plasma	1289:1300	Our data illustrate-particularly in HELP-treated patients-significant decreases of essential omega-6 and omega-3 polyunsaturated fatty acids in blood plasma but significant increases of PUFA-derived lipoxygenase-, as well as cyclooxygenase- and cytochrome P450-derived lipid mediators.
30744123	6	28	theme	mediators	1420:1428	arg1	increases					1318:1326	significant increases	1306:1326	significant increases of PUFA-derived lipoxygenase-, as well as cyclooxygenase- and cytochrome P450-derived lipid mediators	1306:1428	Our data illustrate-particularly in HELP-treated patients-significant decreases of essential omega-6 and omega-3 polyunsaturated fatty acids in blood plasma but significant increases of PUFA-derived lipoxygenase-, as well as cyclooxygenase- and cytochrome P450-derived lipid mediators.
30744123	1	29	with	patients	153:160	arg1	disease					183:189	atherosclerotic disease	167:189	atherosclerotic disease	167:189	Lipoprotein apheresis reliably reduces low-density lipoprotein (LDL) cholesterol in patients with atherosclerotic disease and therapy-refractory hypercholesterolemia or elevated lipoprotein (a) (Lp(a)).
30744123	1	29	with	patients	153:160	arg1	hypercholesterolemia					214:233	therapy-refractory hypercholesterolemia	195:233	therapy-refractory hypercholesterolemia	195:233	Lipoprotein apheresis reliably reduces low-density lipoprotein (LDL) cholesterol in patients with atherosclerotic disease and therapy-refractory hypercholesterolemia or elevated lipoprotein (a) (Lp(a)).
30744123	1	29	with	patients	153:160	arg1	lipoprotein					247:257	elevated lipoprotein	238:257	elevated lipoprotein (a) (Lp(a))	238:269	Lipoprotein apheresis reliably reduces low-density lipoprotein (LDL) cholesterol in patients with atherosclerotic disease and therapy-refractory hypercholesterolemia or elevated lipoprotein (a) (Lp(a)).
30744123	1	29	with	patients	153:160	arg1	a					260:260	a	260:260	a	260:260	Lipoprotein apheresis reliably reduces low-density lipoprotein (LDL) cholesterol in patients with atherosclerotic disease and therapy-refractory hypercholesterolemia or elevated lipoprotein (a) (Lp(a)).
30744123	5	30	theme	gas	962:964	arg1	chromatography					966:979	gas chromatography	962:979	gas chromatography	962:979	Using gas chromatography and liquid chromatography tandem mass spectrometry (LC-MS/MS) we were able to analyze fatty acid composition and the formation of lipid mediators called oxylipins.
30744123	2	31	theme	fatty	402:406	arg1	acids					408:412	polyunsaturated fatty acids	386:412	polyunsaturated fatty acids (n-6 and n-3 PUFAs)	386:432	Besides lowering lipoproteins and triglycerides, apheresis also decreases levels of essential omega-6 and omega-3 polyunsaturated fatty acids (n-6 and n-3 PUFAs) in blood plasma.
30744123	2	31	theme	fatty	402:406	arg1	PUFAs					427:431	n-6 and n-3 PUFAs	415:431	PUFAs	427:431	Besides lowering lipoproteins and triglycerides, apheresis also decreases levels of essential omega-6 and omega-3 polyunsaturated fatty acids (n-6 and n-3 PUFAs) in blood plasma.
30744123	0	32	theme	Lipid	14:18	arg1	Formation					29:37	Lipid Mediator Formation	14:37	Lipid Mediator Formation	14:37	Activation of Lipid Mediator Formation Due to Lipoprotein Apheresis.
30744123	7	33	theme	PUFA	1604:1607	arg1	supplementation					1609:1623	n-3 PUFA supplementation	1600:1623	n-3 PUFA supplementation in the context of lipid apheresis treatment	1600:1667	Given that n-3 PUFAs in particular are presumed to be cardioprotective and n-3 PUFA-derived lipid mediators might limit inflammatory reactions, these data indicate that n-3 PUFA supplementation in the context of lipid apheresis treatment might have additional benefits through apheresis-triggered protective n-3 PUFA-derived lipid mediators.
30744123	1	34	theme	lipoprotein	120:130	arg1	cholesterol					138:148	low-density lipoprotein (LDL) cholesterol	108:148	low-density lipoprotein (LDL) cholesterol in patients with atherosclerotic disease and therapy-refractory hypercholesterolemia or elevated lipoprotein (a) (Lp(a))	108:269	Lipoprotein apheresis reliably reduces low-density lipoprotein (LDL) cholesterol in patients with atherosclerotic disease and therapy-refractory hypercholesterolemia or elevated lipoprotein (a) (Lp(a)).
30744123	7	35	theme	lipid	1756:1760	arg1	mediators					1762:1770	apheresis-triggered protective n-3 PUFA-derived lipid mediators	1708:1770	apheresis-triggered protective n-3 PUFA-derived lipid mediators	1708:1770	Given that n-3 PUFAs in particular are presumed to be cardioprotective and n-3 PUFA-derived lipid mediators might limit inflammatory reactions, these data indicate that n-3 PUFA supplementation in the context of lipid apheresis treatment might have additional benefits through apheresis-triggered protective n-3 PUFA-derived lipid mediators.
30744123	0	36	theme	Formation	29:37	arg1	Activation					0:9	Activation	0:9	Activation of Lipid Mediator Formation	0:37	Activation of Lipid Mediator Formation Due to Lipoprotein Apheresis.
30744123	3	37	theme	n-3	673:675	arg1	PUFAs					677:681	n-6 and n-3 PUFAs	665:681	PUFAs	677:681	In contrast, heparin-induced extracorporeal low-density lipoprotein precipitation (HELP) lipid apheresis might increase the formation of potentially pro-inflammatory and pro-thrombotic lipid mediators derived from n-6 and n-3 PUFAs.
30744123	4	38	theme	direct	854:859	arg1	DA					873:874	DA	873:874	DA	873:874	The study presented here analyzed lipid mediator profiles in the plasma of patients with hyperlipidemia treated by one of three different apheresis methods, either HELP, direct absorption (DA), or membrane filtration (MDF), in a direct pre- and post-apheresis comparison.
30744123	4	38	theme	direct	854:859	arg1	absorption					861:870	direct absorption	854:870	direct absorption (DA)	854:875	The study presented here analyzed lipid mediator profiles in the plasma of patients with hyperlipidemia treated by one of three different apheresis methods, either HELP, direct absorption (DA), or membrane filtration (MDF), in a direct pre- and post-apheresis comparison.
30744123	3	39	theme	low-density	495:505	arg1	lipoprotein					507:517	extracorporeal low-density lipoprotein	480:517	heparin-induced extracorporeal low-density lipoprotein precipitation (HELP) lipid apheresis	464:554	In contrast, heparin-induced extracorporeal low-density lipoprotein precipitation (HELP) lipid apheresis might increase the formation of potentially pro-inflammatory and pro-thrombotic lipid mediators derived from n-6 and n-3 PUFAs.
30744123	4	40	theme	apheresis	822:830	arg1	methods					832:838	one of three different apheresis methods	799:838	one of three different apheresis methods	799:838	The study presented here analyzed lipid mediator profiles in the plasma of patients with hyperlipidemia treated by one of three different apheresis methods, either HELP, direct absorption (DA), or membrane filtration (MDF), in a direct pre- and post-apheresis comparison.
30744123	3	41	theme	heparin-induced	464:478	arg1	apheresis					546:554	heparin-induced extracorporeal low-density lipoprotein precipitation (HELP) lipid apheresis	464:554	heparin-induced extracorporeal low-density lipoprotein precipitation (HELP) lipid apheresis	464:554	In contrast, heparin-induced extracorporeal low-density lipoprotein precipitation (HELP) lipid apheresis might increase the formation of potentially pro-inflammatory and pro-thrombotic lipid mediators derived from n-6 and n-3 PUFAs.
30744123	5	42	theme	chromatography	992:1005	arg1	LC-MS/MS					1033:1040	LC-MS/MS	1033:1040	LC-MS/MS	1033:1040	Using gas chromatography and liquid chromatography tandem mass spectrometry (LC-MS/MS) we were able to analyze fatty acid composition and the formation of lipid mediators called oxylipins.
30744123	5	42	theme	chromatography	992:1005	arg1	spectrometry					1019:1030	liquid chromatography tandem mass spectrometry	985:1030	liquid chromatography tandem mass spectrometry (LC-MS/MS)	985:1041	Using gas chromatography and liquid chromatography tandem mass spectrometry (LC-MS/MS) we were able to analyze fatty acid composition and the formation of lipid mediators called oxylipins.
30744123	2	43	theme	omega-3	378:384	arg1	levels					346:351	levels	346:351	levels of essential omega-6 and omega-3 polyunsaturated fatty acids (n-6 and n-3 PUFAs) in blood plasma	346:448	Besides lowering lipoproteins and triglycerides, apheresis also decreases levels of essential omega-6 and omega-3 polyunsaturated fatty acids (n-6 and n-3 PUFAs) in blood plasma.
30744123	7	44	theme	inflammatory	1551:1562	arg1	reactions					1564:1572	inflammatory reactions	1551:1572	inflammatory reactions	1551:1572	Given that n-3 PUFAs in particular are presumed to be cardioprotective and n-3 PUFA-derived lipid mediators might limit inflammatory reactions, these data indicate that n-3 PUFA supplementation in the context of lipid apheresis treatment might have additional benefits through apheresis-triggered protective n-3 PUFA-derived lipid mediators.
30744123	5	45	theme	mass	1014:1017	arg1	LC-MS/MS					1033:1040	LC-MS/MS	1033:1040	LC-MS/MS	1033:1040	Using gas chromatography and liquid chromatography tandem mass spectrometry (LC-MS/MS) we were able to analyze fatty acid composition and the formation of lipid mediators called oxylipins.
30744123	5	45	theme	mass	1014:1017	arg1	spectrometry					1019:1030	liquid chromatography tandem mass spectrometry	985:1030	liquid chromatography tandem mass spectrometry (LC-MS/MS)	985:1041	Using gas chromatography and liquid chromatography tandem mass spectrometry (LC-MS/MS) we were able to analyze fatty acid composition and the formation of lipid mediators called oxylipins.
30744123	6	46	theme	PUFA-derived	1331:1342	arg1	mediators					1420:1428	PUFA-derived lipoxygenase-, as well as cyclooxygenase- and cytochrome P450-derived lipid mediators	1331:1428	PUFA-derived lipoxygenase-, as well as cyclooxygenase- and cytochrome P450-derived lipid mediators	1331:1428	Our data illustrate-particularly in HELP-treated patients-significant decreases of essential omega-6 and omega-3 polyunsaturated fatty acids in blood plasma but significant increases of PUFA-derived lipoxygenase-, as well as cyclooxygenase- and cytochrome P450-derived lipid mediators.
30744123	3	47	theme	precipitation	519:531	arg1	apheresis					546:554	heparin-induced extracorporeal low-density lipoprotein precipitation (HELP) lipid apheresis	464:554	heparin-induced extracorporeal low-density lipoprotein precipitation (HELP) lipid apheresis	464:554	In contrast, heparin-induced extracorporeal low-density lipoprotein precipitation (HELP) lipid apheresis might increase the formation of potentially pro-inflammatory and pro-thrombotic lipid mediators derived from n-6 and n-3 PUFAs.
30744123	7	48	theme	lipid	1523:1527	arg1	mediators					1529:1537	n-3 PUFA-derived lipid mediators	1506:1537	n-3 PUFA-derived lipid mediators	1506:1537	Given that n-3 PUFAs in particular are presumed to be cardioprotective and n-3 PUFA-derived lipid mediators might limit inflammatory reactions, these data indicate that n-3 PUFA supplementation in the context of lipid apheresis treatment might have additional benefits through apheresis-triggered protective n-3 PUFA-derived lipid mediators.
30744123	4	49	theme	membrane	881:888	arg1	MDF					902:904	MDF	902:904	MDF	902:904	The study presented here analyzed lipid mediator profiles in the plasma of patients with hyperlipidemia treated by one of three different apheresis methods, either HELP, direct absorption (DA), or membrane filtration (MDF), in a direct pre- and post-apheresis comparison.
30744123	4	49	theme	membrane	881:888	arg1	filtration					890:899	membrane filtration	881:899	membrane filtration (MDF)	881:905	The study presented here analyzed lipid mediator profiles in the plasma of patients with hyperlipidemia treated by one of three different apheresis methods, either HELP, direct absorption (DA), or membrane filtration (MDF), in a direct pre- and post-apheresis comparison.
30744123	6	50	theme	patients-significant	1194:1213	arg1	decreases					1215:1223	HELP-treated patients-significant decreases	1181:1223	HELP-treated patients-significant decreases of essential omega-6 and omega-3 polyunsaturated fatty acids in blood plasma	1181:1300	Our data illustrate-particularly in HELP-treated patients-significant decreases of essential omega-6 and omega-3 polyunsaturated fatty acids in blood plasma but significant increases of PUFA-derived lipoxygenase-, as well as cyclooxygenase- and cytochrome P450-derived lipid mediators.
30744123	3	51	theme	lipid	540:544	arg1	apheresis					546:554	heparin-induced extracorporeal low-density lipoprotein precipitation (HELP) lipid apheresis	464:554	heparin-induced extracorporeal low-density lipoprotein precipitation (HELP) lipid apheresis	464:554	In contrast, heparin-induced extracorporeal low-density lipoprotein precipitation (HELP) lipid apheresis might increase the formation of potentially pro-inflammatory and pro-thrombotic lipid mediators derived from n-6 and n-3 PUFAs.
30744123	7	52	from	supplementation	1609:1623	arg1	context					1632:1638	the context	1628:1638	the context of lipid apheresis treatment	1628:1667	Given that n-3 PUFAs in particular are presumed to be cardioprotective and n-3 PUFA-derived lipid mediators might limit inflammatory reactions, these data indicate that n-3 PUFA supplementation in the context of lipid apheresis treatment might have additional benefits through apheresis-triggered protective n-3 PUFA-derived lipid mediators.
30744123	6	53	link	PUFA-derived	1331:1342	arg1	mediators					1420:1428	PUFA-derived lipoxygenase-, as well as cyclooxygenase- and cytochrome P450-derived lipid mediators	1331:1428	PUFA-derived lipoxygenase-, as well as cyclooxygenase- and cytochrome P450-derived lipid mediators	1331:1428	Our data illustrate-particularly in HELP-treated patients-significant decreases of essential omega-6 and omega-3 polyunsaturated fatty acids in blood plasma but significant increases of PUFA-derived lipoxygenase-, as well as cyclooxygenase- and cytochrome P450-derived lipid mediators.
30744123	6	54	theme	blood	1289:1293	arg1	plasma					1295:1300	blood plasma	1289:1300	blood plasma	1289:1300	Our data illustrate-particularly in HELP-treated patients-significant decreases of essential omega-6 and omega-3 polyunsaturated fatty acids in blood plasma but significant increases of PUFA-derived lipoxygenase-, as well as cyclooxygenase- and cytochrome P450-derived lipid mediators.
30744123	1	55	theme	therapy-refractory	195:212	arg1	hypercholesterolemia					214:233	therapy-refractory hypercholesterolemia	195:233	therapy-refractory hypercholesterolemia	195:233	Lipoprotein apheresis reliably reduces low-density lipoprotein (LDL) cholesterol in patients with atherosclerotic disease and therapy-refractory hypercholesterolemia or elevated lipoprotein (a) (Lp(a)).
30744123	7	56	theme	n-3	1739:1741	arg1	mediators					1762:1770	apheresis-triggered protective n-3 PUFA-derived lipid mediators	1708:1770	apheresis-triggered protective n-3 PUFA-derived lipid mediators	1708:1770	Given that n-3 PUFAs in particular are presumed to be cardioprotective and n-3 PUFA-derived lipid mediators might limit inflammatory reactions, these data indicate that n-3 PUFA supplementation in the context of lipid apheresis treatment might have additional benefits through apheresis-triggered protective n-3 PUFA-derived lipid mediators.
30744123	7	57	theme	apheresis-triggered	1708:1726	arg1	mediators					1762:1770	apheresis-triggered protective n-3 PUFA-derived lipid mediators	1708:1770	apheresis-triggered protective n-3 PUFA-derived lipid mediators	1708:1770	Given that n-3 PUFAs in particular are presumed to be cardioprotective and n-3 PUFA-derived lipid mediators might limit inflammatory reactions, these data indicate that n-3 PUFA supplementation in the context of lipid apheresis treatment might have additional benefits through apheresis-triggered protective n-3 PUFA-derived lipid mediators.
30744123	6	58	theme	cyclooxygenase-	1370:1384	arg1	mediators					1420:1428	PUFA-derived lipoxygenase-, as well as cyclooxygenase- and cytochrome P450-derived lipid mediators	1331:1428	PUFA-derived lipoxygenase-, as well as cyclooxygenase- and cytochrome P450-derived lipid mediators	1331:1428	Our data illustrate-particularly in HELP-treated patients-significant decreases of essential omega-6 and omega-3 polyunsaturated fatty acids in blood plasma but significant increases of PUFA-derived lipoxygenase-, as well as cyclooxygenase- and cytochrome P450-derived lipid mediators.
30744123	5	59	theme	fatty	1067:1071	arg1	composition					1078:1088	fatty acid composition	1067:1088	fatty acid composition	1067:1088	Using gas chromatography and liquid chromatography tandem mass spectrometry (LC-MS/MS) we were able to analyze fatty acid composition and the formation of lipid mediators called oxylipins.
30744123	3	60	theme	pro-thrombotic	621:634	arg1	mediators					642:650	potentially pro-inflammatory and pro-thrombotic lipid mediators	588:650	potentially pro-inflammatory and pro-thrombotic lipid mediators derived from n-6 and n-3 PUFAs	588:681	In contrast, heparin-induced extracorporeal low-density lipoprotein precipitation (HELP) lipid apheresis might increase the formation of potentially pro-inflammatory and pro-thrombotic lipid mediators derived from n-6 and n-3 PUFAs.
30744123	4	61	theme	direct	913:918	arg1	comparison					944:953	a direct pre- and post-apheresis comparison	911:953	a direct pre- and post-apheresis comparison	911:953	The study presented here analyzed lipid mediator profiles in the plasma of patients with hyperlipidemia treated by one of three different apheresis methods, either HELP, direct absorption (DA), or membrane filtration (MDF), in a direct pre- and post-apheresis comparison.
30744123	6	62	theme	fatty	1274:1278	arg1	acids					1280:1284	essential omega-6 and omega-3 polyunsaturated fatty acids	1228:1284	acids	1280:1284	Our data illustrate-particularly in HELP-treated patients-significant decreases of essential omega-6 and omega-3 polyunsaturated fatty acids in blood plasma but significant increases of PUFA-derived lipoxygenase-, as well as cyclooxygenase- and cytochrome P450-derived lipid mediators.
30744123	3	63	theme	pro-inflammatory	600:615	arg1	mediators					642:650	potentially pro-inflammatory and pro-thrombotic lipid mediators	588:650	potentially pro-inflammatory and pro-thrombotic lipid mediators derived from n-6 and n-3 PUFAs	588:681	In contrast, heparin-induced extracorporeal low-density lipoprotein precipitation (HELP) lipid apheresis might increase the formation of potentially pro-inflammatory and pro-thrombotic lipid mediators derived from n-6 and n-3 PUFAs.
30744123	4	64	with	patients	759:766	arg1	hyperlipidemia					773:786	hyperlipidemia	773:786	hyperlipidemia	773:786	The study presented here analyzed lipid mediator profiles in the plasma of patients with hyperlipidemia treated by one of three different apheresis methods, either HELP, direct absorption (DA), or membrane filtration (MDF), in a direct pre- and post-apheresis comparison.
30744123	1	65	dep	lipoprotein	247:257	arg1	a					267:267	a	267:267	a	267:267	Lipoprotein apheresis reliably reduces low-density lipoprotein (LDL) cholesterol in patients with atherosclerotic disease and therapy-refractory hypercholesterolemia or elevated lipoprotein (a) (Lp(a)).
30744123	1	65	dep	lipoprotein	247:257	arg1	Lp					264:265	Lp	264:265	Lp(a)	264:268	Lipoprotein apheresis reliably reduces low-density lipoprotein (LDL) cholesterol in patients with atherosclerotic disease and therapy-refractory hypercholesterolemia or elevated lipoprotein (a) (Lp(a)).
30744123	7	66	theme	treatment	1659:1667	arg1	context					1632:1638	the context	1628:1638	the context of lipid apheresis treatment	1628:1667	Given that n-3 PUFAs in particular are presumed to be cardioprotective and n-3 PUFA-derived lipid mediators might limit inflammatory reactions, these data indicate that n-3 PUFA supplementation in the context of lipid apheresis treatment might have additional benefits through apheresis-triggered protective n-3 PUFA-derived lipid mediators.
30744123	6	67	theme	omega-6	1238:1244	arg1	decreases					1215:1223	HELP-treated patients-significant decreases	1181:1223	HELP-treated patients-significant decreases of essential omega-6 and omega-3 polyunsaturated fatty acids in blood plasma	1181:1300	Our data illustrate-particularly in HELP-treated patients-significant decreases of essential omega-6 and omega-3 polyunsaturated fatty acids in blood plasma but significant increases of PUFA-derived lipoxygenase-, as well as cyclooxygenase- and cytochrome P450-derived lipid mediators.
30744123	3	68	theme	mediators	642:650	arg1	formation					575:583	the formation	571:583	the formation of potentially pro-inflammatory and pro-thrombotic lipid mediators derived from n-6 and n-3 PUFAs	571:681	In contrast, heparin-induced extracorporeal low-density lipoprotein precipitation (HELP) lipid apheresis might increase the formation of potentially pro-inflammatory and pro-thrombotic lipid mediators derived from n-6 and n-3 PUFAs.
30744123	7	69	theme	lipid	1643:1647	arg1	treatment					1659:1667	lipid apheresis treatment	1643:1667	lipid apheresis treatment	1643:1667	Given that n-3 PUFAs in particular are presumed to be cardioprotective and n-3 PUFA-derived lipid mediators might limit inflammatory reactions, these data indicate that n-3 PUFA supplementation in the context of lipid apheresis treatment might have additional benefits through apheresis-triggered protective n-3 PUFA-derived lipid mediators.
30744123	5	70	theme	mediators	1117:1125	arg1	formation					1098:1106	the formation	1094:1106	the formation of lipid mediators called oxylipins	1094:1142	Using gas chromatography and liquid chromatography tandem mass spectrometry (LC-MS/MS) we were able to analyze fatty acid composition and the formation of lipid mediators called oxylipins.
30744123	5	70	theme	mediators	1117:1125	arg1	composition					1078:1088	fatty acid composition	1067:1088	fatty acid composition	1067:1088	Using gas chromatography and liquid chromatography tandem mass spectrometry (LC-MS/MS) we were able to analyze fatty acid composition and the formation of lipid mediators called oxylipins.
30744123	2	71	from	plasma	443:448	arg1	levels					346:351	levels	346:351	levels of essential omega-6 and omega-3 polyunsaturated fatty acids (n-6 and n-3 PUFAs) in blood plasma	346:448	Besides lowering lipoproteins and triglycerides, apheresis also decreases levels of essential omega-6 and omega-3 polyunsaturated fatty acids (n-6 and n-3 PUFAs) in blood plasma.
30744123	4	72	theme	mediator	724:731	arg1	profiles					733:740	lipid mediator profiles	718:740	lipid mediator profiles in the plasma of patients with hyperlipidemia treated by one of three different apheresis methods, either HELP, direct absorption (DA), or membrane filtration (MDF)	718:905	The study presented here analyzed lipid mediator profiles in the plasma of patients with hyperlipidemia treated by one of three different apheresis methods, either HELP, direct absorption (DA), or membrane filtration (MDF), in a direct pre- and post-apheresis comparison.
30744123	7	73	contain	have	1675:1678	arg2	benefits					1691:1698	additional benefits	1680:1698	additional benefits	1680:1698	Given that n-3 PUFAs in particular are presumed to be cardioprotective and n-3 PUFA-derived lipid mediators might limit inflammatory reactions, these data indicate that n-3 PUFA supplementation in the context of lipid apheresis treatment might have additional benefits through apheresis-triggered protective n-3 PUFA-derived lipid mediators.
30744123	7	73	contain	have	1675:1678	arg1	supplementation					1609:1623	n-3 PUFA supplementation	1600:1623	n-3 PUFA supplementation in the context of lipid apheresis treatment	1600:1667	Given that n-3 PUFAs in particular are presumed to be cardioprotective and n-3 PUFA-derived lipid mediators might limit inflammatory reactions, these data indicate that n-3 PUFA supplementation in the context of lipid apheresis treatment might have additional benefits through apheresis-triggered protective n-3 PUFA-derived lipid mediators.
30744123	6	74	theme	lipid	1414:1418	arg1	mediators					1420:1428	PUFA-derived lipoxygenase-, as well as cyclooxygenase- and cytochrome P450-derived lipid mediators	1331:1428	PUFA-derived lipoxygenase-, as well as cyclooxygenase- and cytochrome P450-derived lipid mediators	1331:1428	Our data illustrate-particularly in HELP-treated patients-significant decreases of essential omega-6 and omega-3 polyunsaturated fatty acids in blood plasma but significant increases of PUFA-derived lipoxygenase-, as well as cyclooxygenase- and cytochrome P450-derived lipid mediators.
30744123	1	75	theme	Lipoprotein	69:79	arg1	apheresis					81:89	Lipoprotein apheresis	69:89	Lipoprotein apheresis	69:89	Lipoprotein apheresis reliably reduces low-density lipoprotein (LDL) cholesterol in patients with atherosclerotic disease and therapy-refractory hypercholesterolemia or elevated lipoprotein (a) (Lp(a)).
30744123	6	76	from	decreases	1215:1223	arg1	plasma					1295:1300	blood plasma	1289:1300	blood plasma	1289:1300	Our data illustrate-particularly in HELP-treated patients-significant decreases of essential omega-6 and omega-3 polyunsaturated fatty acids in blood plasma but significant increases of PUFA-derived lipoxygenase-, as well as cyclooxygenase- and cytochrome P450-derived lipid mediators.
30744123	2	77	theme	acids	408:412	arg1	levels					346:351	levels	346:351	levels of essential omega-6 and omega-3 polyunsaturated fatty acids (n-6 and n-3 PUFAs) in blood plasma	346:448	Besides lowering lipoproteins and triglycerides, apheresis also decreases levels of essential omega-6 and omega-3 polyunsaturated fatty acids (n-6 and n-3 PUFAs) in blood plasma.
30744123	1	78	from	cholesterol	138:148	arg1	patients					153:160	patients	153:160	patients with atherosclerotic disease and therapy-refractory hypercholesterolemia or elevated lipoprotein (a) (Lp(a))	153:269	Lipoprotein apheresis reliably reduces low-density lipoprotein (LDL) cholesterol in patients with atherosclerotic disease and therapy-refractory hypercholesterolemia or elevated lipoprotein (a) (Lp(a)).
30744123	3	79	dep	precipitation	519:531	arg1	HELP					534:537	HELP	534:537	HELP	534:537	In contrast, heparin-induced extracorporeal low-density lipoprotein precipitation (HELP) lipid apheresis might increase the formation of potentially pro-inflammatory and pro-thrombotic lipid mediators derived from n-6 and n-3 PUFAs.
30744123	2	80	theme	blood	437:441	arg1	plasma					443:448	blood plasma	437:448	blood plasma	437:448	Besides lowering lipoproteins and triglycerides, apheresis also decreases levels of essential omega-6 and omega-3 polyunsaturated fatty acids (n-6 and n-3 PUFAs) in blood plasma.
30744123	4	81	theme	patients	759:766	arg1	DA					873:874	DA	873:874	DA	873:874	The study presented here analyzed lipid mediator profiles in the plasma of patients with hyperlipidemia treated by one of three different apheresis methods, either HELP, direct absorption (DA), or membrane filtration (MDF), in a direct pre- and post-apheresis comparison.
30744123	4	81	theme	patients	759:766	arg1	absorption					861:870	direct absorption	854:870	direct absorption (DA)	854:875	The study presented here analyzed lipid mediator profiles in the plasma of patients with hyperlipidemia treated by one of three different apheresis methods, either HELP, direct absorption (DA), or membrane filtration (MDF), in a direct pre- and post-apheresis comparison.
30744123	4	81	theme	patients	759:766	arg1	plasma					749:754	the plasma	745:754	the plasma of patients with hyperlipidemia treated by one of three different apheresis methods	745:838	The study presented here analyzed lipid mediator profiles in the plasma of patients with hyperlipidemia treated by one of three different apheresis methods, either HELP, direct absorption (DA), or membrane filtration (MDF), in a direct pre- and post-apheresis comparison.
30744123	4	81	theme	patients	759:766	arg1	MDF					902:904	MDF	902:904	MDF	902:904	The study presented here analyzed lipid mediator profiles in the plasma of patients with hyperlipidemia treated by one of three different apheresis methods, either HELP, direct absorption (DA), or membrane filtration (MDF), in a direct pre- and post-apheresis comparison.
30744123	4	81	theme	patients	759:766	arg1	filtration					890:899	membrane filtration	881:899	membrane filtration (MDF)	881:905	The study presented here analyzed lipid mediator profiles in the plasma of patients with hyperlipidemia treated by one of three different apheresis methods, either HELP, direct absorption (DA), or membrane filtration (MDF), in a direct pre- and post-apheresis comparison.
30744123	2	82	theme	polyunsaturated	386:400	arg1	acids					408:412	polyunsaturated fatty acids	386:412	polyunsaturated fatty acids (n-6 and n-3 PUFAs)	386:432	Besides lowering lipoproteins and triglycerides, apheresis also decreases levels of essential omega-6 and omega-3 polyunsaturated fatty acids (n-6 and n-3 PUFAs) in blood plasma.
30744123	2	82	theme	polyunsaturated	386:400	arg1	PUFAs					427:431	n-6 and n-3 PUFAs	415:431	PUFAs	427:431	Besides lowering lipoproteins and triglycerides, apheresis also decreases levels of essential omega-6 and omega-3 polyunsaturated fatty acids (n-6 and n-3 PUFAs) in blood plasma.
30744123	0	83	theme	Mediator	20:27	arg1	Formation					29:37	Lipid Mediator Formation	14:37	Lipid Mediator Formation	14:37	Activation of Lipid Mediator Formation Due to Lipoprotein Apheresis.
30744123	1	84	theme	low-density	108:118	arg1	LDL					133:135	LDL	133:135	LDL	133:135	Lipoprotein apheresis reliably reduces low-density lipoprotein (LDL) cholesterol in patients with atherosclerotic disease and therapy-refractory hypercholesterolemia or elevated lipoprotein (a) (Lp(a)).
30744123	1	84	theme	low-density	108:118	arg1	lipoprotein					120:130	low-density lipoprotein	108:130	low-density lipoprotein (LDL) cholesterol in patients with atherosclerotic disease and therapy-refractory hypercholesterolemia or elevated lipoprotein (a) (Lp(a))	108:269	Lipoprotein apheresis reliably reduces low-density lipoprotein (LDL) cholesterol in patients with atherosclerotic disease and therapy-refractory hypercholesterolemia or elevated lipoprotein (a) (Lp(a)).
30744123	7	85	theme	n-3	1442:1444	arg1	PUFAs					1446:1450	n-3 PUFAs	1442:1450	n-3 PUFAs in particular	1442:1464	Given that n-3 PUFAs in particular are presumed to be cardioprotective and n-3 PUFA-derived lipid mediators might limit inflammatory reactions, these data indicate that n-3 PUFA supplementation in the context of lipid apheresis treatment might have additional benefits through apheresis-triggered protective n-3 PUFA-derived lipid mediators.
30744123	2	86	theme	n-3	423:425	arg1	acids					408:412	polyunsaturated fatty acids	386:412	polyunsaturated fatty acids (n-6 and n-3 PUFAs)	386:432	Besides lowering lipoproteins and triglycerides, apheresis also decreases levels of essential omega-6 and omega-3 polyunsaturated fatty acids (n-6 and n-3 PUFAs) in blood plasma.
30744123	2	86	theme	n-3	423:425	arg1	PUFAs					427:431	n-6 and n-3 PUFAs	415:431	PUFAs	427:431	Besides lowering lipoproteins and triglycerides, apheresis also decreases levels of essential omega-6 and omega-3 polyunsaturated fatty acids (n-6 and n-3 PUFAs) in blood plasma.
30744123	7	87	from	PUFAs	1446:1450	arg1	particular					1455:1464	particular	1455:1464	particular	1455:1464	Given that n-3 PUFAs in particular are presumed to be cardioprotective and n-3 PUFA-derived lipid mediators might limit inflammatory reactions, these data indicate that n-3 PUFA supplementation in the context of lipid apheresis treatment might have additional benefits through apheresis-triggered protective n-3 PUFA-derived lipid mediators.
30744123	2	88	theme	n-6	415:417	arg1	acids					408:412	polyunsaturated fatty acids	386:412	polyunsaturated fatty acids (n-6 and n-3 PUFAs)	386:432	Besides lowering lipoproteins and triglycerides, apheresis also decreases levels of essential omega-6 and omega-3 polyunsaturated fatty acids (n-6 and n-3 PUFAs) in blood plasma.
30744123	2	88	theme	n-6	415:417	arg1	PUFAs					427:431	n-6 and n-3 PUFAs	415:431	PUFAs	427:431	Besides lowering lipoproteins and triglycerides, apheresis also decreases levels of essential omega-6 and omega-3 polyunsaturated fatty acids (n-6 and n-3 PUFAs) in blood plasma.
30744123	0	89	theme	Lipoprotein	46:56	arg1	Apheresis					58:66	Lipoprotein Apheresis	46:66	Lipoprotein Apheresis	46:66	Activation of Lipid Mediator Formation Due to Lipoprotein Apheresis.
30744123	7	90	theme	n-3	1600:1602	arg1	PUFA					1604:1607	n-3 PUFA	1600:1607	n-3 PUFA supplementation in the context of lipid apheresis treatment	1600:1667	Given that n-3 PUFAs in particular are presumed to be cardioprotective and n-3 PUFA-derived lipid mediators might limit inflammatory reactions, these data indicate that n-3 PUFA supplementation in the context of lipid apheresis treatment might have additional benefits through apheresis-triggered protective n-3 PUFA-derived lipid mediators.
30744123	3	91	theme	lipoprotein	507:517	arg1	precipitation					519:531	extracorporeal low-density lipoprotein precipitation	480:531	heparin-induced extracorporeal low-density lipoprotein precipitation (HELP) lipid apheresis	464:554	In contrast, heparin-induced extracorporeal low-density lipoprotein precipitation (HELP) lipid apheresis might increase the formation of potentially pro-inflammatory and pro-thrombotic lipid mediators derived from n-6 and n-3 PUFAs.
30744123	0	92	dep	Activation	0:9	arg1	Apheresis					58:66	Lipoprotein Apheresis	46:66	Lipoprotein Apheresis	46:66	Activation of Lipid Mediator Formation Due to Lipoprotein Apheresis.
30744123	5	93	theme	liquid	985:990	arg1	LC-MS/MS					1033:1040	LC-MS/MS	1033:1040	LC-MS/MS	1033:1040	Using gas chromatography and liquid chromatography tandem mass spectrometry (LC-MS/MS) we were able to analyze fatty acid composition and the formation of lipid mediators called oxylipins.
30744123	5	93	theme	liquid	985:990	arg1	spectrometry					1019:1030	liquid chromatography tandem mass spectrometry	985:1030	liquid chromatography tandem mass spectrometry (LC-MS/MS)	985:1041	Using gas chromatography and liquid chromatography tandem mass spectrometry (LC-MS/MS) we were able to analyze fatty acid composition and the formation of lipid mediators called oxylipins.
30744123	2	94	from	acids	408:412	arg1	plasma					443:448	blood plasma	437:448	blood plasma	437:448	Besides lowering lipoproteins and triglycerides, apheresis also decreases levels of essential omega-6 and omega-3 polyunsaturated fatty acids (n-6 and n-3 PUFAs) in blood plasma.
30744123	4	95	theme	different	812:820	arg1	methods					832:838	one of three different apheresis methods	799:838	one of three different apheresis methods	799:838	The study presented here analyzed lipid mediator profiles in the plasma of patients with hyperlipidemia treated by one of three different apheresis methods, either HELP, direct absorption (DA), or membrane filtration (MDF), in a direct pre- and post-apheresis comparison.
30744123	3	96	theme	extracorporeal	480:493	arg1	lipoprotein					507:517	extracorporeal low-density lipoprotein	480:517	heparin-induced extracorporeal low-density lipoprotein precipitation (HELP) lipid apheresis	464:554	In contrast, heparin-induced extracorporeal low-density lipoprotein precipitation (HELP) lipid apheresis might increase the formation of potentially pro-inflammatory and pro-thrombotic lipid mediators derived from n-6 and n-3 PUFAs.
30744123	5	97	theme	tandem	1007:1012	arg1	LC-MS/MS					1033:1040	LC-MS/MS	1033:1040	LC-MS/MS	1033:1040	Using gas chromatography and liquid chromatography tandem mass spectrometry (LC-MS/MS) we were able to analyze fatty acid composition and the formation of lipid mediators called oxylipins.
30744123	5	97	theme	tandem	1007:1012	arg1	spectrometry					1019:1030	liquid chromatography tandem mass spectrometry	985:1030	liquid chromatography tandem mass spectrometry (LC-MS/MS)	985:1041	Using gas chromatography and liquid chromatography tandem mass spectrometry (LC-MS/MS) we were able to analyze fatty acid composition and the formation of lipid mediators called oxylipins.
30744123	4	98	from	profiles	733:740	arg1	absorption					861:870	direct absorption	854:870	direct absorption (DA)	854:875	The study presented here analyzed lipid mediator profiles in the plasma of patients with hyperlipidemia treated by one of three different apheresis methods, either HELP, direct absorption (DA), or membrane filtration (MDF), in a direct pre- and post-apheresis comparison.
30744123	4	98	from	profiles	733:740	arg1	plasma					749:754	the plasma	745:754	the plasma of patients with hyperlipidemia treated by one of three different apheresis methods	745:838	The study presented here analyzed lipid mediator profiles in the plasma of patients with hyperlipidemia treated by one of three different apheresis methods, either HELP, direct absorption (DA), or membrane filtration (MDF), in a direct pre- and post-apheresis comparison.
30744123	4	98	from	profiles	733:740	arg1	DA					873:874	DA	873:874	DA	873:874	The study presented here analyzed lipid mediator profiles in the plasma of patients with hyperlipidemia treated by one of three different apheresis methods, either HELP, direct absorption (DA), or membrane filtration (MDF), in a direct pre- and post-apheresis comparison.
30744123	4	98	from	profiles	733:740	arg1	MDF					902:904	MDF	902:904	MDF	902:904	The study presented here analyzed lipid mediator profiles in the plasma of patients with hyperlipidemia treated by one of three different apheresis methods, either HELP, direct absorption (DA), or membrane filtration (MDF), in a direct pre- and post-apheresis comparison.
30744123	4	98	from	profiles	733:740	arg1	HELP					848:851	HELP	848:851	HELP	848:851	The study presented here analyzed lipid mediator profiles in the plasma of patients with hyperlipidemia treated by one of three different apheresis methods, either HELP, direct absorption (DA), or membrane filtration (MDF), in a direct pre- and post-apheresis comparison.
30744123	4	98	from	profiles	733:740	arg1	filtration					890:899	membrane filtration	881:899	membrane filtration (MDF)	881:905	The study presented here analyzed lipid mediator profiles in the plasma of patients with hyperlipidemia treated by one of three different apheresis methods, either HELP, direct absorption (DA), or membrane filtration (MDF), in a direct pre- and post-apheresis comparison.
31745602	4	0	theme	Flavobacterium	435:448	arg1	IMCC26223T					461:470	Flavobacterium soyangense IMCC26223T	435:470	Flavobacterium soyangense IMCC26223T (97.0%)	435:478	Phylogenetic analyses using 16S rRNA showed that strain GS13T is a member of the genus Flavobacterium, with highest similarity to Flavobacterium soyangense IMCC26223T (97.0%).
31745602	4	0	theme	Flavobacterium	435:448	arg1	%					477:477	97.0%	473:477	97.0%	473:477	Phylogenetic analyses using 16S rRNA showed that strain GS13T is a member of the genus Flavobacterium, with highest similarity to Flavobacterium soyangense IMCC26223T (97.0%).
31745602	8	1	theme	predominant	778:788	arg1	MK-6					814:817	MK-6	814:817	MK-6	814:817	The predominant respiratory quinone was MK-6.
31745602	8	1	theme	predominant	778:788	arg1	quinone					802:808	The predominant respiratory quinone	774:808	The predominant respiratory quinone	774:808	The predominant respiratory quinone was MK-6.
31745602	5	2	theme	36.2 mol	521:528	arg1	%					529:529	36.2 mol%	521:529	36.2 mol%	521:529	The DNA G+C content of strain GS13T was 36.2 mol%.
31745602	5	2	theme	36.2 mol	521:528	arg1	content					493:499	The DNA G+C content	481:499	The DNA G+C content of strain GS13T	481:515	The DNA G+C content of strain GS13T was 36.2 mol%.
31745602	6	3	theme	fatty	545:549	arg1	acids					551:555	The dominant fatty acids	532:555	The dominant fatty acids	532:555	The dominant fatty acids were summed feature 3 (C16:1ω7c and/or C16:1ω6c) and iso-C15:0.
31745602	6	3	theme	fatty	545:549	arg1	feature					569:575	summed feature 3	562:577	summed feature 3 (C16:1ω7c and/or C16:1ω6c)	562:604	The dominant fatty acids were summed feature 3 (C16:1ω7c and/or C16:1ω6c) and iso-C15:0.
31745602	9	4	theme	closely-related	885:899	arg1	species					916:922	closely-related Flavobacterium species	885:922	closely-related Flavobacterium species	885:922	Our data demonstrate that strain GS13T can be distinguished from closely-related Flavobacterium species.
31745602	2	5	dep	Gram-negative	96:108	arg1	rod-shaped					123:132	rod-shaped	123:132	rod-shaped	123:132	A Gram-negative, non-motile, rod-shaped bacterial strain, designated GS13T, was isolated from sediments in a branch of the Nackdong River in Sangju, Korea.
31745602	2	5	dep	Gram-negative	96:108	arg1	non-motile					111:120	non-motile	111:120	non-motile	111:120	A Gram-negative, non-motile, rod-shaped bacterial strain, designated GS13T, was isolated from sediments in a branch of the Nackdong River in Sangju, Korea.
31745602	6	6	theme	dominant	536:543	arg1	acids					551:555	The dominant fatty acids	532:555	The dominant fatty acids	532:555	The dominant fatty acids were summed feature 3 (C16:1ω7c and/or C16:1ω6c) and iso-C15:0.
31745602	6	6	theme	dominant	536:543	arg1	feature					569:575	summed feature 3	562:577	summed feature 3 (C16:1ω7c and/or C16:1ω6c)	562:604	The dominant fatty acids were summed feature 3 (C16:1ω7c and/or C16:1ω6c) and iso-C15:0.
31745602	7	7	theme	major	625:629	arg1	lipids					637:642	The major polar lipids	621:642	The major polar lipids	621:642	The major polar lipids were phosphatidylethanolamine, three unidentified aminolipids, three unidentified lipids, and one unidentified aminophospholipid.
31745602	7	7	theme	major	625:629	arg1	phosphatidylethanolamine					649:672	phosphatidylethanolamine	649:672	phosphatidylethanolamine	649:672	The major polar lipids were phosphatidylethanolamine, three unidentified aminolipids, three unidentified lipids, and one unidentified aminophospholipid.
31745602	2	8	from	sediments	188:196	arg1	branch					203:208	a branch	201:208	a branch of the Nackdong River in Sangju, Korea	201:247	A Gram-negative, non-motile, rod-shaped bacterial strain, designated GS13T, was isolated from sediments in a branch of the Nackdong River in Sangju, Korea.
31745602	4	9	theme	highest	413:419	arg1	similarity					421:430	highest similarity	413:430	highest similarity to Flavobacterium soyangense IMCC26223T (97.0%)	413:478	Phylogenetic analyses using 16S rRNA showed that strain GS13T is a member of the genus Flavobacterium, with highest similarity to Flavobacterium soyangense IMCC26223T (97.0%).
31745602	12	10	theme	type	1044:1047	arg1	GS13T					1059:1063	GS13T	1059:1063	GS13T (=KCTC 62569T = JCM 32765T)	1059:1091	The type strain is GS13T (=KCTC 62569T = JCM 32765T).
31745602	12	10	theme	type	1044:1047	arg1	strain					1049:1054	The type strain	1040:1054	The type strain	1040:1054	The type strain is GS13T (=KCTC 62569T = JCM 32765T).
31745602	7	11	theme	polar	631:635	arg1	lipids					637:642	The major polar lipids	621:642	The major polar lipids	621:642	The major polar lipids were phosphatidylethanolamine, three unidentified aminolipids, three unidentified lipids, and one unidentified aminophospholipid.
31745602	7	11	theme	polar	631:635	arg1	phosphatidylethanolamine					649:672	phosphatidylethanolamine	649:672	phosphatidylethanolamine	649:672	The major polar lipids were phosphatidylethanolamine, three unidentified aminolipids, three unidentified lipids, and one unidentified aminophospholipid.
31745602	3	12	theme	%	297:297	arg1	NaCl					299:302	0% NaCl	296:302	0% NaCl	296:302	Optimal growth occurred at pH 7.0, 20 °C, and 0% NaCl.
31745602	4	13	theme	strain	354:359	arg1	member					372:377	a member	370:377	a member of the genus Flavobacterium	370:405	Phylogenetic analyses using 16S rRNA showed that strain GS13T is a member of the genus Flavobacterium, with highest similarity to Flavobacterium soyangense IMCC26223T (97.0%).
31745602	4	13	theme	strain	354:359	arg1	GS13T					361:365	strain GS13T	354:365	strain GS13T	354:365	Phylogenetic analyses using 16S rRNA showed that strain GS13T is a member of the genus Flavobacterium, with highest similarity to Flavobacterium soyangense IMCC26223T (97.0%).
31745602	12	14	theme	62569T = JCM	1072:1083	arg1	32765T					1085:1090	=KCTC 62569T = JCM 32765T	1066:1090	=KCTC 62569T = JCM 32765T	1066:1090	The type strain is GS13T (=KCTC 62569T = JCM 32765T).
31745602	12	14	theme	62569T = JCM	1072:1083	arg1	GS13T					1059:1063	GS13T	1059:1063	GS13T (=KCTC 62569T = JCM 32765T)	1059:1091	The type strain is GS13T (=KCTC 62569T = JCM 32765T).
31745602	2	15	from	River	226:230	arg1	Korea					243:247	Korea	243:247	Korea	243:247	A Gram-negative, non-motile, rod-shaped bacterial strain, designated GS13T, was isolated from sediments in a branch of the Nackdong River in Sangju, Korea.
31745602	2	16	from	branch	203:208	arg1	Korea					243:247	Korea	243:247	Korea	243:247	A Gram-negative, non-motile, rod-shaped bacterial strain, designated GS13T, was isolated from sediments in a branch of the Nackdong River in Sangju, Korea.
31745602	0	17	theme	nackdongense	15:26	arg1	sp					28:29	Flavobacterium nackdongense sp	0:29	Flavobacterium nackdongense sp	0:29	Flavobacterium nackdongense sp.
31745602	5	18	theme	strain	504:509	arg1	GS13T					511:515	strain GS13T	504:515	strain GS13T	504:515	The DNA G+C content of strain GS13T was 36.2 mol%.
31745602	0	19	theme	Flavobacterium	0:13	arg1	sp					28:29	Flavobacterium nackdongense sp	0:29	Flavobacterium nackdongense sp	0:29	Flavobacterium nackdongense sp.
31745602	1	20	attach	isolated	70:77	arg2	bacterium					60:68	a cellulose-degrading bacterium	38:68	a cellulose-degrading bacterium isolated from sediment	38:91	nov., a cellulose-degrading bacterium isolated from sediment.
31745602	1	20	attach	isolated	70:77	arg1	sediment					84:91	sediment	84:91	sediment	84:91	nov., a cellulose-degrading bacterium isolated from sediment.
31745602	5	21	theme	GS13T	511:515	arg1	%					529:529	36.2 mol%	521:529	36.2 mol%	521:529	The DNA G+C content of strain GS13T was 36.2 mol%.
31745602	5	21	theme	GS13T	511:515	arg1	content					493:499	The DNA G+C content	481:499	The DNA G+C content of strain GS13T	481:515	The DNA G+C content of strain GS13T was 36.2 mol%.
31745602	4	22	theme	16S	333:335	arg1	rRNA					337:340	16S rRNA	333:340	16S rRNA	333:340	Phylogenetic analyses using 16S rRNA showed that strain GS13T is a member of the genus Flavobacterium, with highest similarity to Flavobacterium soyangense IMCC26223T (97.0%).
31745602	2	23	attach	isolated	174:181	arg2	strain					144:149	A Gram-negative, non-motile, rod-shaped bacterial strain	94:149	A Gram-negative, non-motile, rod-shaped bacterial strain	94:149	A Gram-negative, non-motile, rod-shaped bacterial strain, designated GS13T, was isolated from sediments in a branch of the Nackdong River in Sangju, Korea.
31745602	2	23	attach	isolated	174:181	arg1	sediments					188:196	sediments	188:196	sediments in a branch of the Nackdong River in Sangju, Korea	188:247	A Gram-negative, non-motile, rod-shaped bacterial strain, designated GS13T, was isolated from sediments in a branch of the Nackdong River in Sangju, Korea.
31745602	5	24	theme	G+C	489:491	arg1	%					529:529	36.2 mol%	521:529	36.2 mol%	521:529	The DNA G+C content of strain GS13T was 36.2 mol%.
31745602	5	24	theme	G+C	489:491	arg1	content					493:499	The DNA G+C content	481:499	The DNA G+C content of strain GS13T	481:515	The DNA G+C content of strain GS13T was 36.2 mol%.
31745602	4	25	theme	genus	386:390	arg1	Flavobacterium					392:405	the genus Flavobacterium	382:405	the genus Flavobacterium	382:405	Phylogenetic analyses using 16S rRNA showed that strain GS13T is a member of the genus Flavobacterium, with highest similarity to Flavobacterium soyangense IMCC26223T (97.0%).
31745602	2	26	from	Korea	243:247	arg1	branch					203:208	a branch	201:208	a branch of the Nackdong River in Sangju, Korea	201:247	A Gram-negative, non-motile, rod-shaped bacterial strain, designated GS13T, was isolated from sediments in a branch of the Nackdong River in Sangju, Korea.
31745602	4	27	with	member	372:377	arg1	similarity					421:430	highest similarity	413:430	highest similarity to Flavobacterium soyangense IMCC26223T (97.0%)	413:478	Phylogenetic analyses using 16S rRNA showed that strain GS13T is a member of the genus Flavobacterium, with highest similarity to Flavobacterium soyangense IMCC26223T (97.0%).
31745602	2	28	theme	River	226:230	arg1	branch					203:208	a branch	201:208	a branch of the Nackdong River in Sangju, Korea	201:247	A Gram-negative, non-motile, rod-shaped bacterial strain, designated GS13T, was isolated from sediments in a branch of the Nackdong River in Sangju, Korea.
31745602	3	29	theme	Optimal	250:256	arg1	growth					258:263	Optimal growth	250:263	Optimal growth	250:263	Optimal growth occurred at pH 7.0, 20 °C, and 0% NaCl.
31745602	2	30	theme	Nackdong	217:224	arg1	River					226:230	the Nackdong River	213:230	the Nackdong River in Sangju, Korea	213:247	A Gram-negative, non-motile, rod-shaped bacterial strain, designated GS13T, was isolated from sediments in a branch of the Nackdong River in Sangju, Korea.
31745602	9	31	theme	Flavobacterium	901:914	arg1	species					916:922	closely-related Flavobacterium species	885:922	closely-related Flavobacterium species	885:922	Our data demonstrate that strain GS13T can be distinguished from closely-related Flavobacterium species.
31745602	12	32	theme	=KCTC	1066:1070	arg1	32765T					1085:1090	=KCTC 62569T = JCM 32765T	1066:1090	=KCTC 62569T = JCM 32765T	1066:1090	The type strain is GS13T (=KCTC 62569T = JCM 32765T).
31745602	12	32	theme	=KCTC	1066:1070	arg1	GS13T					1059:1063	GS13T	1059:1063	GS13T (=KCTC 62569T = JCM 32765T)	1059:1091	The type strain is GS13T (=KCTC 62569T = JCM 32765T).
31745602	2	33	theme	Gram-negative	96:108	arg1	strain					144:149	A Gram-negative, non-motile, rod-shaped bacterial strain	94:149	A Gram-negative, non-motile, rod-shaped bacterial strain	94:149	A Gram-negative, non-motile, rod-shaped bacterial strain, designated GS13T, was isolated from sediments in a branch of the Nackdong River in Sangju, Korea.
31745602	4	34	theme	Flavobacterium	392:405	arg1	member					372:377	a member	370:377	a member of the genus Flavobacterium	370:405	Phylogenetic analyses using 16S rRNA showed that strain GS13T is a member of the genus Flavobacterium, with highest similarity to Flavobacterium soyangense IMCC26223T (97.0%).
31745602	4	34	theme	Flavobacterium	392:405	arg1	GS13T					361:365	strain GS13T	354:365	strain GS13T	354:365	Phylogenetic analyses using 16S rRNA showed that strain GS13T is a member of the genus Flavobacterium, with highest similarity to Flavobacterium soyangense IMCC26223T (97.0%).
31745602	5	35	theme	DNA	485:487	arg1	%					529:529	36.2 mol%	521:529	36.2 mol%	521:529	The DNA G+C content of strain GS13T was 36.2 mol%.
31745602	5	35	theme	DNA	485:487	arg1	content					493:499	The DNA G+C content	481:499	The DNA G+C content of strain GS13T	481:515	The DNA G+C content of strain GS13T was 36.2 mol%.
31745602	10	36	theme	nackdongense	1018:1029	arg1	sp					1031:1032	the name Flavobacterium nackdongense sp	994:1032	the name Flavobacterium nackdongense sp	994:1032	Thus, strain GS13T is a novel Flavobacterium species, and we propose the name Flavobacterium nackdongense sp.
31745602	2	37	theme	bacterial	134:142	arg1	strain					144:149	A Gram-negative, non-motile, rod-shaped bacterial strain	94:149	A Gram-negative, non-motile, rod-shaped bacterial strain	94:149	A Gram-negative, non-motile, rod-shaped bacterial strain, designated GS13T, was isolated from sediments in a branch of the Nackdong River in Sangju, Korea.
31745602	10	38	theme	Flavobacterium	1003:1016	arg1	sp					1031:1032	the name Flavobacterium nackdongense sp	994:1032	the name Flavobacterium nackdongense sp	994:1032	Thus, strain GS13T is a novel Flavobacterium species, and we propose the name Flavobacterium nackdongense sp.
31745602	4	39	theme	Phylogenetic	305:316	arg1	analyses					318:325	Phylogenetic analyses	305:325	Phylogenetic analyses using 16S rRNA	305:340	Phylogenetic analyses using 16S rRNA showed that strain GS13T is a member of the genus Flavobacterium, with highest similarity to Flavobacterium soyangense IMCC26223T (97.0%).
31745602	9	40	theme	strain	846:851	arg1	GS13T					853:857	strain GS13T	846:857	strain GS13T	846:857	Our data demonstrate that strain GS13T can be distinguished from closely-related Flavobacterium species.
31745602	10	41	theme	name	998:1001	arg1	sp					1031:1032	the name Flavobacterium nackdongense sp	994:1032	the name Flavobacterium nackdongense sp	994:1032	Thus, strain GS13T is a novel Flavobacterium species, and we propose the name Flavobacterium nackdongense sp.
31745602	10	42	theme	Flavobacterium	955:968	arg1	species					970:976	a novel Flavobacterium species	947:976	a novel Flavobacterium species	947:976	Thus, strain GS13T is a novel Flavobacterium species, and we propose the name Flavobacterium nackdongense sp.
31745602	10	42	theme	Flavobacterium	955:968	arg1	GS13T					938:942	strain GS13T	931:942	strain GS13T	931:942	Thus, strain GS13T is a novel Flavobacterium species, and we propose the name Flavobacterium nackdongense sp.
31745602	10	43	theme	strain	931:936	arg1	species					970:976	a novel Flavobacterium species	947:976	a novel Flavobacterium species	947:976	Thus, strain GS13T is a novel Flavobacterium species, and we propose the name Flavobacterium nackdongense sp.
31745602	10	43	theme	strain	931:936	arg1	GS13T					938:942	strain GS13T	931:942	strain GS13T	931:942	Thus, strain GS13T is a novel Flavobacterium species, and we propose the name Flavobacterium nackdongense sp.
31745602	1	44	dep	bacterium	60:68	arg1	nov.					32:35	nov.	32:35	nov.	32:35	nov., a cellulose-degrading bacterium isolated from sediment.
31745602	10	45	theme	novel	949:953	arg1	species					970:976	a novel Flavobacterium species	947:976	a novel Flavobacterium species	947:976	Thus, strain GS13T is a novel Flavobacterium species, and we propose the name Flavobacterium nackdongense sp.
31745602	10	45	theme	novel	949:953	arg1	GS13T					938:942	strain GS13T	931:942	strain GS13T	931:942	Thus, strain GS13T is a novel Flavobacterium species, and we propose the name Flavobacterium nackdongense sp.
31745602	6	46	dep	feature	569:575	arg1	C16:1ω6c					596:603	C16:1ω6c	596:603	C16:1ω6c	596:603	The dominant fatty acids were summed feature 3 (C16:1ω7c and/or C16:1ω6c) and iso-C15:0.
31745602	6	46	dep	feature	569:575	arg1	C16:1ω7c					580:587	C16:1ω7c	580:587	C16:1ω7c	580:587	The dominant fatty acids were summed feature 3 (C16:1ω7c and/or C16:1ω6c) and iso-C15:0.
31745602	7	47	theme	unidentified	681:692	arg1	aminolipids					694:704	three unidentified aminolipids	675:704	three unidentified aminolipids	675:704	The major polar lipids were phosphatidylethanolamine, three unidentified aminolipids, three unidentified lipids, and one unidentified aminophospholipid.
31745602	7	47	theme	unidentified	681:692	arg1	phosphatidylethanolamine					649:672	phosphatidylethanolamine	649:672	phosphatidylethanolamine	649:672	The major polar lipids were phosphatidylethanolamine, three unidentified aminolipids, three unidentified lipids, and one unidentified aminophospholipid.
31745602	6	48	theme	summed	562:567	arg1	acids					551:555	The dominant fatty acids	532:555	The dominant fatty acids	532:555	The dominant fatty acids were summed feature 3 (C16:1ω7c and/or C16:1ω6c) and iso-C15:0.
31745602	6	48	theme	summed	562:567	arg1	feature					569:575	summed feature 3	562:577	summed feature 3 (C16:1ω7c and/or C16:1ω6c)	562:604	The dominant fatty acids were summed feature 3 (C16:1ω7c and/or C16:1ω6c) and iso-C15:0.
31745602	3	49	theme	0	296:296	arg1	%					297:297	%	297:297	%	297:297	Optimal growth occurred at pH 7.0, 20 °C, and 0% NaCl.
31745602	4	50	theme	soyangense	450:459	arg1	IMCC26223T					461:470	Flavobacterium soyangense IMCC26223T	435:470	Flavobacterium soyangense IMCC26223T (97.0%)	435:478	Phylogenetic analyses using 16S rRNA showed that strain GS13T is a member of the genus Flavobacterium, with highest similarity to Flavobacterium soyangense IMCC26223T (97.0%).
31745602	4	50	theme	soyangense	450:459	arg1	%					477:477	97.0%	473:477	97.0%	473:477	Phylogenetic analyses using 16S rRNA showed that strain GS13T is a member of the genus Flavobacterium, with highest similarity to Flavobacterium soyangense IMCC26223T (97.0%).
31745602	1	51	theme	cellulose-degrading	40:58	arg1	bacterium					60:68	a cellulose-degrading bacterium	38:68	a cellulose-degrading bacterium isolated from sediment	38:91	nov., a cellulose-degrading bacterium isolated from sediment.
31745602	8	52	theme	respiratory	790:800	arg1	MK-6					814:817	MK-6	814:817	MK-6	814:817	The predominant respiratory quinone was MK-6.
31745602	8	52	theme	respiratory	790:800	arg1	quinone					802:808	The predominant respiratory quinone	774:808	The predominant respiratory quinone	774:808	The predominant respiratory quinone was MK-6.
31745602	7	53	theme	unidentified	713:724	arg1	phosphatidylethanolamine					649:672	phosphatidylethanolamine	649:672	phosphatidylethanolamine	649:672	The major polar lipids were phosphatidylethanolamine, three unidentified aminolipids, three unidentified lipids, and one unidentified aminophospholipid.
31745602	7	53	theme	unidentified	713:724	arg1	lipids					726:731	three unidentified lipids	707:731	three unidentified lipids	707:731	The major polar lipids were phosphatidylethanolamine, three unidentified aminolipids, three unidentified lipids, and one unidentified aminophospholipid.
30172300	6	0	from	reduction	963:971	arg1	bacteria					988:995	recoverable bacteria	976:995	recoverable bacteria	976:995	There was a 3-4 log reduction in recoverable bacteria when antimicrobial dressings were applied to biofilms cultured for 48 h, and left in situ for a further 24 h. Two-species biofilms of S. aureus and P. aeruginosa inoculated at a 1:1 ratio, were also reproducibly cultured at both 20 °C and 37 °C; of particular note was a definitive Gram-negative shift within the population that occurred only at 37 °C.
30172300	5	1	theme	72 h	873:876	arg1	counts					900:905	72 h giving consistent log counts	873:905	72 h giving consistent log counts of 8-10 colony forming units (CFU)	873:940	Single species biofilms of Staphylococcus aureus or Pseudomonas aeruginosa were reproducibly cultured over 72 h giving consistent log counts of 8-10 colony forming units (CFU).
30172300	6	2	theme	3-4	955:957	arg1	reduction					963:971	a 3-4 log reduction	953:971	a 3-4 log reduction in recoverable bacteria	953:995	There was a 3-4 log reduction in recoverable bacteria when antimicrobial dressings were applied to biofilms cultured for 48 h, and left in situ for a further 24 h. Two-species biofilms of S. aureus and P. aeruginosa inoculated at a 1:1 ratio, were also reproducibly cultured at both 20 °C and 37 °C; of particular note was a definitive Gram-negative shift within the population that occurred only at 37 °C.
30172300	0	3	theme	wound	77:81	arg1	infection					83:91	chronic wound infection	69:91	chronic wound infection	69:91	A novel flow-system to establish experimental biofilms for modelling chronic wound infection and testing the efficacy of wound dressings.
30172300	1	4	with	culture	294:300	arg1	possibility					311:321	the possibility	307:321	the possibility of applying topical antimicrobial treatments	307:366	Several models exist for the study of chronic wound infection, but few combine all of the necessary elements to allow high throughput, reproducible biofilm culture with the possibility of applying topical antimicrobial treatments.
30172300	5	5	theme	giving	878:883	arg1	counts					900:905	72 h giving consistent log counts	873:905	72 h giving consistent log counts of 8-10 colony forming units (CFU)	873:940	Single species biofilms of Staphylococcus aureus or Pseudomonas aeruginosa were reproducibly cultured over 72 h giving consistent log counts of 8-10 colony forming units (CFU).
30172300	1	6	theme	necessary	228:236	arg1	elements					238:245	the necessary elements	224:245	the necessary elements	224:245	Several models exist for the study of chronic wound infection, but few combine all of the necessary elements to allow high throughput, reproducible biofilm culture with the possibility of applying topical antimicrobial treatments.
30172300	6	7	dep	cultured	1209:1216	arg1	left					1074:1077	left	1074:1077	left	1074:1077	There was a 3-4 log reduction in recoverable bacteria when antimicrobial dressings were applied to biofilms cultured for 48 h, and left in situ for a further 24 h. Two-species biofilms of S. aureus and P. aeruginosa inoculated at a 1:1 ratio, were also reproducibly cultured at both 20 °C and 37 °C; of particular note was a definitive Gram-negative shift within the population that occurred only at 37 °C.
30172300	3	8	theme	new	529:531	arg1	device					546:551	a new biofilm flow device	527:551	a new biofilm flow device for study of wound biofilms	527:579	In this manuscript, a new biofilm flow device for study of wound biofilms is reported.
30172300	6	9	theme	24 h.	1101:1105	arg1	biofilms					1119:1126	a further 24 h. Two-species biofilms	1091:1126	a further 24 h. Two-species biofilms of S. aureus and P. aeruginosa inoculated at a 1:1 ratio	1091:1183	There was a 3-4 log reduction in recoverable bacteria when antimicrobial dressings were applied to biofilms cultured for 48 h, and left in situ for a further 24 h. Two-species biofilms of S. aureus and P. aeruginosa inoculated at a 1:1 ratio, were also reproducibly cultured at both 20 °C and 37 °C; of particular note was a definitive Gram-negative shift within the population that occurred only at 37 °C.
30172300	1	10	theme	topical	335:341	arg1	treatments					357:366	topical antimicrobial treatments	335:366	topical antimicrobial treatments	335:366	Several models exist for the study of chronic wound infection, but few combine all of the necessary elements to allow high throughput, reproducible biofilm culture with the possibility of applying topical antimicrobial treatments.
30172300	3	11	theme	biofilm	533:539	arg1	device					546:551	a new biofilm flow device	527:551	a new biofilm flow device for study of wound biofilms	527:579	In this manuscript, a new biofilm flow device for study of wound biofilms is reported.
30172300	6	12	theme	definitive	1268:1277	arg1	shift					1293:1297	a definitive Gram-negative shift	1266:1297	a definitive Gram-negative shift	1266:1297	There was a 3-4 log reduction in recoverable bacteria when antimicrobial dressings were applied to biofilms cultured for 48 h, and left in situ for a further 24 h. Two-species biofilms of S. aureus and P. aeruginosa inoculated at a 1:1 ratio, were also reproducibly cultured at both 20 °C and 37 °C; of particular note was a definitive Gram-negative shift within the population that occurred only at 37 °C.
30172300	1	13	theme	antimicrobial	343:355	arg1	treatments					357:366	topical antimicrobial treatments	335:366	topical antimicrobial treatments	335:366	Several models exist for the study of chronic wound infection, but few combine all of the necessary elements to allow high throughput, reproducible biofilm culture with the possibility of applying topical antimicrobial treatments.
30172300	6	14	theme	antimicrobial	1002:1014	arg1	dressings					1016:1024	antimicrobial dressings	1002:1024	antimicrobial dressings	1002:1024	There was a 3-4 log reduction in recoverable bacteria when antimicrobial dressings were applied to biofilms cultured for 48 h, and left in situ for a further 24 h. Two-species biofilms of S. aureus and P. aeruginosa inoculated at a 1:1 ratio, were also reproducibly cultured at both 20 °C and 37 °C; of particular note was a definitive Gram-negative shift within the population that occurred only at 37 °C.
30172300	5	15	theme	Single	766:771	arg1	biofilms					781:788	Single species biofilms	766:788	Single species biofilms of Staphylococcus aureus or Pseudomonas aeruginosa	766:839	Single species biofilms of Staphylococcus aureus or Pseudomonas aeruginosa were reproducibly cultured over 72 h giving consistent log counts of 8-10 colony forming units (CFU).
30172300	5	16	theme	aeruginosa	830:839	arg1	biofilms					781:788	Single species biofilms	766:788	Single species biofilms of Staphylococcus aureus or Pseudomonas aeruginosa	766:839	Single species biofilms of Staphylococcus aureus or Pseudomonas aeruginosa were reproducibly cultured over 72 h giving consistent log counts of 8-10 colony forming units (CFU).
30172300	0	17	theme	novel	2:6	arg1	flow-system					8:18	A novel flow-system	0:18	A novel flow-system	0:18	A novel flow-system to establish experimental biofilms for modelling chronic wound infection and testing the efficacy of wound dressings.
30172300	4	18	used	used	659:662	arg2	device					598:603	The device	594:603	The device	594:603	The device is 3D printed, straightforward to operate, and can be used to investigate single and mixed species biofilms, as well as the efficacy of antimicrobial dressings.
30172300	4	19	theme	species	696:702	arg1	biofilms					704:711	single and mixed species biofilms	679:711	single and mixed species biofilms	679:711	The device is 3D printed, straightforward to operate, and can be used to investigate single and mixed species biofilms, as well as the efficacy of antimicrobial dressings.
30172300	2	20	theme	providing	429:437	arg1	nutrients					439:447	providing nutrients	429:447	providing nutrients combined with biofilm growth at the air-liquid interface	429:504	Furthermore, few take into account the appropriate means of providing nutrients combined with biofilm growth at the air-liquid interface.
30172300	4	21	theme	mixed	690:694	arg1	biofilms					704:711	single and mixed species biofilms	679:711	single and mixed species biofilms	679:711	The device is 3D printed, straightforward to operate, and can be used to investigate single and mixed species biofilms, as well as the efficacy of antimicrobial dressings.
30172300	1	22	theme	chronic	176:182	arg1	infection					190:198	chronic wound infection	176:198	chronic wound infection	176:198	Several models exist for the study of chronic wound infection, but few combine all of the necessary elements to allow high throughput, reproducible biofilm culture with the possibility of applying topical antimicrobial treatments.
30172300	5	23	theme	species	773:779	arg1	biofilms					781:788	Single species biofilms	766:788	Single species biofilms of Staphylococcus aureus or Pseudomonas aeruginosa	766:839	Single species biofilms of Staphylococcus aureus or Pseudomonas aeruginosa were reproducibly cultured over 72 h giving consistent log counts of 8-10 colony forming units (CFU).
30172300	1	24	theme	high	256:259	arg1	throughput					261:270	high throughput	256:270	high throughput	256:270	Several models exist for the study of chronic wound infection, but few combine all of the necessary elements to allow high throughput, reproducible biofilm culture with the possibility of applying topical antimicrobial treatments.
30172300	1	24	theme	high	256:259	arg1	culture					294:300	reproducible biofilm culture	273:300	reproducible biofilm culture with the possibility of applying topical antimicrobial treatments	273:366	Several models exist for the study of chronic wound infection, but few combine all of the necessary elements to allow high throughput, reproducible biofilm culture with the possibility of applying topical antimicrobial treatments.
30172300	0	25	theme	wound	121:125	arg1	dressings					127:135	wound dressings	121:135	wound dressings	121:135	A novel flow-system to establish experimental biofilms for modelling chronic wound infection and testing the efficacy of wound dressings.
30172300	3	26	theme	flow	541:544	arg1	device					546:551	a new biofilm flow device	527:551	a new biofilm flow device for study of wound biofilms	527:579	In this manuscript, a new biofilm flow device for study of wound biofilms is reported.
30172300	6	27	theme	1:1	1175:1177	arg1	ratio					1179:1183	a 1:1 ratio	1173:1183	a 1:1 ratio	1173:1183	There was a 3-4 log reduction in recoverable bacteria when antimicrobial dressings were applied to biofilms cultured for 48 h, and left in situ for a further 24 h. Two-species biofilms of S. aureus and P. aeruginosa inoculated at a 1:1 ratio, were also reproducibly cultured at both 20 °C and 37 °C; of particular note was a definitive Gram-negative shift within the population that occurred only at 37 °C.
30172300	2	28	theme	appropriate	408:418	arg1	means					420:424	the appropriate means	404:424	the appropriate means of providing nutrients combined with biofilm growth at the air-liquid interface	404:504	Furthermore, few take into account the appropriate means of providing nutrients combined with biofilm growth at the air-liquid interface.
30172300	5	29	theme	colony	915:920	arg1	CFU					937:939	CFU	937:939	CFU	937:939	Single species biofilms of Staphylococcus aureus or Pseudomonas aeruginosa were reproducibly cultured over 72 h giving consistent log counts of 8-10 colony forming units (CFU).
30172300	5	29	theme	colony	915:920	arg1	units					930:934	8-10 colony forming units	910:934	8-10 colony forming units (CFU)	910:940	Single species biofilms of Staphylococcus aureus or Pseudomonas aeruginosa were reproducibly cultured over 72 h giving consistent log counts of 8-10 colony forming units (CFU).
30172300	6	30	theme	note	1257:1260	arg1	shift					1293:1297	a definitive Gram-negative shift	1266:1297	a definitive Gram-negative shift	1266:1297	There was a 3-4 log reduction in recoverable bacteria when antimicrobial dressings were applied to biofilms cultured for 48 h, and left in situ for a further 24 h. Two-species biofilms of S. aureus and P. aeruginosa inoculated at a 1:1 ratio, were also reproducibly cultured at both 20 °C and 37 °C; of particular note was a definitive Gram-negative shift within the population that occurred only at 37 °C.
30172300	5	31	theme	units	930:934	arg1	counts					900:905	72 h giving consistent log counts	873:905	72 h giving consistent log counts of 8-10 colony forming units (CFU)	873:940	Single species biofilms of Staphylococcus aureus or Pseudomonas aeruginosa were reproducibly cultured over 72 h giving consistent log counts of 8-10 colony forming units (CFU).
30172300	1	32	theme	infection	190:198	arg1	study					167:171	the study	163:171	the study of chronic wound infection	163:198	Several models exist for the study of chronic wound infection, but few combine all of the necessary elements to allow high throughput, reproducible biofilm culture with the possibility of applying topical antimicrobial treatments.
30172300	0	33	theme	experimental	33:44	arg1	biofilms					46:53	experimental biofilms	33:53	experimental biofilms	33:53	A novel flow-system to establish experimental biofilms for modelling chronic wound infection and testing the efficacy of wound dressings.
30172300	6	34	theme	particular	1246:1255	arg1	note					1257:1260	particular note	1246:1260	particular note	1246:1260	There was a 3-4 log reduction in recoverable bacteria when antimicrobial dressings were applied to biofilms cultured for 48 h, and left in situ for a further 24 h. Two-species biofilms of S. aureus and P. aeruginosa inoculated at a 1:1 ratio, were also reproducibly cultured at both 20 °C and 37 °C; of particular note was a definitive Gram-negative shift within the population that occurred only at 37 °C.
30172300	3	35	theme	biofilms	572:579	arg1	study					557:561	study	557:561	study of wound biofilms	557:579	In this manuscript, a new biofilm flow device for study of wound biofilms is reported.
30172300	5	36	theme	aureus	808:813	arg1	biofilms					781:788	Single species biofilms	766:788	Single species biofilms of Staphylococcus aureus or Pseudomonas aeruginosa	766:839	Single species biofilms of Staphylococcus aureus or Pseudomonas aeruginosa were reproducibly cultured over 72 h giving consistent log counts of 8-10 colony forming units (CFU).
30172300	2	37	theme	biofilm	463:469	arg1	growth					471:476	biofilm growth	463:476	biofilm growth	463:476	Furthermore, few take into account the appropriate means of providing nutrients combined with biofilm growth at the air-liquid interface.
30172300	1	38	theme	reproducible	273:284	arg1	throughput					261:270	high throughput	256:270	high throughput	256:270	Several models exist for the study of chronic wound infection, but few combine all of the necessary elements to allow high throughput, reproducible biofilm culture with the possibility of applying topical antimicrobial treatments.
30172300	1	38	theme	reproducible	273:284	arg1	culture					294:300	reproducible biofilm culture	273:300	reproducible biofilm culture with the possibility of applying topical antimicrobial treatments	273:366	Several models exist for the study of chronic wound infection, but few combine all of the necessary elements to allow high throughput, reproducible biofilm culture with the possibility of applying topical antimicrobial treatments.
30172300	0	39	theme	dressings	127:135	arg1	efficacy					109:116	the efficacy	105:116	the efficacy of wound dressings	105:135	A novel flow-system to establish experimental biofilms for modelling chronic wound infection and testing the efficacy of wound dressings.
30172300	1	40	theme	biofilm	286:292	arg1	throughput					261:270	high throughput	256:270	high throughput	256:270	Several models exist for the study of chronic wound infection, but few combine all of the necessary elements to allow high throughput, reproducible biofilm culture with the possibility of applying topical antimicrobial treatments.
30172300	1	40	theme	biofilm	286:292	arg1	culture					294:300	reproducible biofilm culture	273:300	reproducible biofilm culture with the possibility of applying topical antimicrobial treatments	273:366	Several models exist for the study of chronic wound infection, but few combine all of the necessary elements to allow high throughput, reproducible biofilm culture with the possibility of applying topical antimicrobial treatments.
30172300	5	41	theme	consistent	885:894	arg1	counts					900:905	72 h giving consistent log counts	873:905	72 h giving consistent log counts of 8-10 colony forming units (CFU)	873:940	Single species biofilms of Staphylococcus aureus or Pseudomonas aeruginosa were reproducibly cultured over 72 h giving consistent log counts of 8-10 colony forming units (CFU).
30172300	3	42	theme	wound	566:570	arg1	biofilms					572:579	wound biofilms	566:579	wound biofilms	566:579	In this manuscript, a new biofilm flow device for study of wound biofilms is reported.
30172300	5	43	theme	log	896:898	arg1	counts					900:905	72 h giving consistent log counts	873:905	72 h giving consistent log counts of 8-10 colony forming units (CFU)	873:940	Single species biofilms of Staphylococcus aureus or Pseudomonas aeruginosa were reproducibly cultured over 72 h giving consistent log counts of 8-10 colony forming units (CFU).
30172300	6	44	theme	aureus	1134:1139	arg1	biofilms					1119:1126	a further 24 h. Two-species biofilms	1091:1126	a further 24 h. Two-species biofilms of S. aureus and P. aeruginosa inoculated at a 1:1 ratio	1091:1183	There was a 3-4 log reduction in recoverable bacteria when antimicrobial dressings were applied to biofilms cultured for 48 h, and left in situ for a further 24 h. Two-species biofilms of S. aureus and P. aeruginosa inoculated at a 1:1 ratio, were also reproducibly cultured at both 20 °C and 37 °C; of particular note was a definitive Gram-negative shift within the population that occurred only at 37 °C.
30172300	6	45	theme	aeruginosa	1148:1157	arg1	biofilms					1119:1126	a further 24 h. Two-species biofilms	1091:1126	a further 24 h. Two-species biofilms of S. aureus and P. aeruginosa inoculated at a 1:1 ratio	1091:1183	There was a 3-4 log reduction in recoverable bacteria when antimicrobial dressings were applied to biofilms cultured for 48 h, and left in situ for a further 24 h. Two-species biofilms of S. aureus and P. aeruginosa inoculated at a 1:1 ratio, were also reproducibly cultured at both 20 °C and 37 °C; of particular note was a definitive Gram-negative shift within the population that occurred only at 37 °C.
30172300	5	46	theme	forming	922:928	arg1	CFU					937:939	CFU	937:939	CFU	937:939	Single species biofilms of Staphylococcus aureus or Pseudomonas aeruginosa were reproducibly cultured over 72 h giving consistent log counts of 8-10 colony forming units (CFU).
30172300	5	46	theme	forming	922:928	arg1	units					930:934	8-10 colony forming units	910:934	8-10 colony forming units (CFU)	910:940	Single species biofilms of Staphylococcus aureus or Pseudomonas aeruginosa were reproducibly cultured over 72 h giving consistent log counts of 8-10 colony forming units (CFU).
30172300	6	47	theme	recoverable	976:986	arg1	bacteria					988:995	recoverable bacteria	976:995	recoverable bacteria	976:995	There was a 3-4 log reduction in recoverable bacteria when antimicrobial dressings were applied to biofilms cultured for 48 h, and left in situ for a further 24 h. Two-species biofilms of S. aureus and P. aeruginosa inoculated at a 1:1 ratio, were also reproducibly cultured at both 20 °C and 37 °C; of particular note was a definitive Gram-negative shift within the population that occurred only at 37 °C.
30172300	6	48	theme	Two-species	1107:1117	arg1	biofilms					1119:1126	a further 24 h. Two-species biofilms	1091:1126	a further 24 h. Two-species biofilms of S. aureus and P. aeruginosa inoculated at a 1:1 ratio	1091:1183	There was a 3-4 log reduction in recoverable bacteria when antimicrobial dressings were applied to biofilms cultured for 48 h, and left in situ for a further 24 h. Two-species biofilms of S. aureus and P. aeruginosa inoculated at a 1:1 ratio, were also reproducibly cultured at both 20 °C and 37 °C; of particular note was a definitive Gram-negative shift within the population that occurred only at 37 °C.
30172300	4	49	theme	single	679:684	arg1	biofilms					704:711	single and mixed species biofilms	679:711	single and mixed species biofilms	679:711	The device is 3D printed, straightforward to operate, and can be used to investigate single and mixed species biofilms, as well as the efficacy of antimicrobial dressings.
30172300	6	50	theme	Gram-negative	1279:1291	arg1	shift					1293:1297	a definitive Gram-negative shift	1266:1297	a definitive Gram-negative shift	1266:1297	There was a 3-4 log reduction in recoverable bacteria when antimicrobial dressings were applied to biofilms cultured for 48 h, and left in situ for a further 24 h. Two-species biofilms of S. aureus and P. aeruginosa inoculated at a 1:1 ratio, were also reproducibly cultured at both 20 °C and 37 °C; of particular note was a definitive Gram-negative shift within the population that occurred only at 37 °C.
30172300	0	51	theme	chronic	69:75	arg1	infection					83:91	chronic wound infection	69:91	chronic wound infection	69:91	A novel flow-system to establish experimental biofilms for modelling chronic wound infection and testing the efficacy of wound dressings.
30172300	4	52	theme	dressings	755:763	arg1	biofilms					704:711	single and mixed species biofilms	679:711	single and mixed species biofilms	679:711	The device is 3D printed, straightforward to operate, and can be used to investigate single and mixed species biofilms, as well as the efficacy of antimicrobial dressings.
30172300	4	52	theme	dressings	755:763	arg1	efficacy					729:736	the efficacy	725:736	the efficacy of antimicrobial dressings	725:763	The device is 3D printed, straightforward to operate, and can be used to investigate single and mixed species biofilms, as well as the efficacy of antimicrobial dressings.
30172300	1	53	theme	wound	184:188	arg1	infection					190:198	chronic wound infection	176:198	chronic wound infection	176:198	Several models exist for the study of chronic wound infection, but few combine all of the necessary elements to allow high throughput, reproducible biofilm culture with the possibility of applying topical antimicrobial treatments.
30172300	1	54	theme	Several	138:144	arg1	models					146:151	Several models	138:151	Several models	138:151	Several models exist for the study of chronic wound infection, but few combine all of the necessary elements to allow high throughput, reproducible biofilm culture with the possibility of applying topical antimicrobial treatments.
30172300	6	55	theme	further	1093:1099	arg1	biofilms					1119:1126	a further 24 h. Two-species biofilms	1091:1126	a further 24 h. Two-species biofilms of S. aureus and P. aeruginosa inoculated at a 1:1 ratio	1091:1183	There was a 3-4 log reduction in recoverable bacteria when antimicrobial dressings were applied to biofilms cultured for 48 h, and left in situ for a further 24 h. Two-species biofilms of S. aureus and P. aeruginosa inoculated at a 1:1 ratio, were also reproducibly cultured at both 20 °C and 37 °C; of particular note was a definitive Gram-negative shift within the population that occurred only at 37 °C.
30172300	2	56	theme	air-liquid	485:494	arg1	interface					496:504	the air-liquid interface	481:504	the air-liquid interface	481:504	Furthermore, few take into account the appropriate means of providing nutrients combined with biofilm growth at the air-liquid interface.
30172300	4	57	theme	antimicrobial	741:753	arg1	dressings					755:763	antimicrobial dressings	741:763	antimicrobial dressings	741:763	The device is 3D printed, straightforward to operate, and can be used to investigate single and mixed species biofilms, as well as the efficacy of antimicrobial dressings.
30172300	2	58	theme	nutrients	439:447	arg1	means					420:424	the appropriate means	404:424	the appropriate means of providing nutrients combined with biofilm growth at the air-liquid interface	404:504	Furthermore, few take into account the appropriate means of providing nutrients combined with biofilm growth at the air-liquid interface.
30172300	6	59	theme	log	959:961	arg1	reduction					963:971	a 3-4 log reduction	953:971	a 3-4 log reduction in recoverable bacteria	953:995	There was a 3-4 log reduction in recoverable bacteria when antimicrobial dressings were applied to biofilms cultured for 48 h, and left in situ for a further 24 h. Two-species biofilms of S. aureus and P. aeruginosa inoculated at a 1:1 ratio, were also reproducibly cultured at both 20 °C and 37 °C; of particular note was a definitive Gram-negative shift within the population that occurred only at 37 °C.
30534686	1	0	theme	MOFs-based	119:128	arg1	hydrogel					130:137	An as-synthesized MOFs-based hydrogel	101:137	An as-synthesized MOFs-based hydrogel	101:137	An as-synthesized MOFs-based hydrogel exhibits high sensitivity for β-lactamase through an "ON-OFF-OFF-ON" luminescence trigger.
30534686	0	1	theme	MOFs-based	35:44	arg1	hydrogel					56:63	a MOFs-based composite hydrogel	33:63	Diagnosis of penicillin allergy: a MOFs-based composite hydrogel for detecting β-lactamase in serum.	0:99	Diagnosis of penicillin allergy: a MOFs-based composite hydrogel for detecting β-lactamase in serum.
30534686	1	2	theme	luminescence	208:219	arg1	trigger					221:227	an "ON-OFF-OFF-ON" luminescence trigger	189:227	an "ON-OFF-OFF-ON" luminescence trigger	189:227	An as-synthesized MOFs-based hydrogel exhibits high sensitivity for β-lactamase through an "ON-OFF-OFF-ON" luminescence trigger.
30534686	2	3	from	detection	333:341	arg1	serum					361:365	serum	361:365	serum	361:365	It allows achieving an unprecedented strategy for the judgement of penicillin allergy via luminescence detection of β-lactamase in serum.
30534686	2	4	theme	β-lactamase	346:356	arg1	detection					333:341	luminescence detection	320:341	luminescence detection of β-lactamase in serum	320:365	It allows achieving an unprecedented strategy for the judgement of penicillin allergy via luminescence detection of β-lactamase in serum.
30534686	2	5	theme	allergy	308:314	arg1	judgement					284:292	the judgement	280:292	the judgement of penicillin allergy	280:314	It allows achieving an unprecedented strategy for the judgement of penicillin allergy via luminescence detection of β-lactamase in serum.
30534686	2	6	theme	unprecedented	253:265	arg1	strategy					267:274	an unprecedented strategy	250:274	an unprecedented strategy for the judgement of penicillin allergy	250:314	It allows achieving an unprecedented strategy for the judgement of penicillin allergy via luminescence detection of β-lactamase in serum.
30534686	0	7	theme	composite	46:54	arg1	hydrogel					56:63	a MOFs-based composite hydrogel	33:63	Diagnosis of penicillin allergy: a MOFs-based composite hydrogel for detecting β-lactamase in serum.	0:99	Diagnosis of penicillin allergy: a MOFs-based composite hydrogel for detecting β-lactamase in serum.
30534686	1	8	theme	high	148:151	arg1	sensitivity					153:163	high sensitivity	148:163	high sensitivity for β-lactamase	148:179	An as-synthesized MOFs-based hydrogel exhibits high sensitivity for β-lactamase through an "ON-OFF-OFF-ON" luminescence trigger.
30534686	1	9	theme	ON-OFF-OFF-ON	193:205	arg1	trigger					221:227	an "ON-OFF-OFF-ON" luminescence trigger	189:227	an "ON-OFF-OFF-ON" luminescence trigger	189:227	An as-synthesized MOFs-based hydrogel exhibits high sensitivity for β-lactamase through an "ON-OFF-OFF-ON" luminescence trigger.
30534686	0	10	theme	allergy	24:30	arg1	Diagnosis					0:8	Diagnosis	0:8	Diagnosis of penicillin allergy: a MOFs-based composite hydrogel for detecting β-lactamase in serum.	0:99	Diagnosis of penicillin allergy: a MOFs-based composite hydrogel for detecting β-lactamase in serum.
30534686	2	11	theme	penicillin	297:306	arg1	allergy					308:314	penicillin allergy	297:314	penicillin allergy	297:314	It allows achieving an unprecedented strategy for the judgement of penicillin allergy via luminescence detection of β-lactamase in serum.
30534686	1	12	theme	as-synthesized	104:117	arg1	hydrogel					130:137	An as-synthesized MOFs-based hydrogel	101:137	An as-synthesized MOFs-based hydrogel	101:137	An as-synthesized MOFs-based hydrogel exhibits high sensitivity for β-lactamase through an "ON-OFF-OFF-ON" luminescence trigger.
30534686	0	13	dep	Diagnosis	0:8	arg1	hydrogel					56:63	a MOFs-based composite hydrogel	33:63	Diagnosis of penicillin allergy: a MOFs-based composite hydrogel for detecting β-lactamase in serum.	0:99	Diagnosis of penicillin allergy: a MOFs-based composite hydrogel for detecting β-lactamase in serum.
30534686	2	14	theme	luminescence	320:331	arg1	detection					333:341	luminescence detection	320:341	luminescence detection of β-lactamase in serum	320:365	It allows achieving an unprecedented strategy for the judgement of penicillin allergy via luminescence detection of β-lactamase in serum.
30534686	1	15	theme	"	206:206	arg1	trigger					221:227	an "ON-OFF-OFF-ON" luminescence trigger	189:227	an "ON-OFF-OFF-ON" luminescence trigger	189:227	An as-synthesized MOFs-based hydrogel exhibits high sensitivity for β-lactamase through an "ON-OFF-OFF-ON" luminescence trigger.
30534686	0	16	theme	penicillin	13:22	arg1	allergy					24:30	penicillin allergy	13:30	penicillin allergy	13:30	Diagnosis of penicillin allergy: a MOFs-based composite hydrogel for detecting β-lactamase in serum.
31432793	12	0	theme	significant	1362:1372	arg1	scavenging					1407:1416	1' diphenyl picryl-hydrazyle scavenging	1378:1416	1' diphenyl picryl-hydrazyle scavenging (80.1%)	1378:1424	Also showed significant 1', 1' diphenyl picryl-hydrazyle scavenging (80.1%) at 15 µg/mL.
31432793	12	0	theme	significant	1362:1372	arg1	%					1423:1423	80.1%	1419:1423	80.1%	1419:1423	Also showed significant 1', 1' diphenyl picryl-hydrazyle scavenging (80.1%) at 15 µg/mL.
31432793	2	1	dep	Fourier	416:422	arg1	transform					424:432	transform	424:432	transform infrared spectroscopy	424:454	Analytical tools: ultraviolet-visible (UV-vis), dynamic light scattering, zeta potential, transmission electron microscope, X-ray diffraction (XRD), and Fourier transform infrared spectroscopy were employed.
31432793	8	2	theme	considerable	929:940	arg1	activity					953:960	considerable peroxidase activity	929:960	considerable peroxidase activity	929:960	NP displayed considerable peroxidase activity via catalysis of 3, 3', 5, 5'-tetramethylbenzidine oxidation by H2O2.
31432793	3	3	theme	Wide	471:474	arg1	absorption					494:503	Wide continuous UV-vis absorption	471:503	Wide continuous UV-vis absorption	471:503	Wide continuous UV-vis absorption and black solution colouration proved Pt NP formation.
31432793	1	4	theme	gum	228:230	arg1	ghatti					232:237	gum ghatti	228:237	gum ghatti (Anogeissus latifolia)	228:260	A single pot, green method for platinum nanoparticles (Pt NP) production was devised with gum ghatti (Anogeissus latifolia).
31432793	13	5	theme	applications	1535:1546	arg1	range					1497:1501	a range	1495:1501	a range of biological and environmental applications	1495:1546	Author envisages that the biogenic Pt NP can be used in a range of biological and environmental applications.
31432793	10	6	with	comparison	1189:1198	arg1	peroxidase					1218:1227	horse radish peroxidase	1205:1227	horse radish peroxidase	1205:1227	In comparison with horse radish peroxidase, its applicability as an artificial peroxidase is advantageous.
31432793	9	7	theme	salt	1115:1118	arg1	concentration					1120:1132	salt concentration	1115:1132	salt concentration (0-100 mM)	1115:1143	NP showed exceptional stability towards varying pH (3-11), temperature (25-100°C), salt concentration (0-100 mM) and storage time duration (0-12 months).
31432793	9	7	theme	salt	1115:1118	arg1	mM					1141:1142	0-100 mM	1135:1142	0-100 mM	1135:1142	NP showed exceptional stability towards varying pH (3-11), temperature (25-100°C), salt concentration (0-100 mM) and storage time duration (0-12 months).
31432793	2	8	theme	light	319:323	arg1	scattering					325:334	dynamic light scattering	311:334	dynamic light scattering	311:334	Analytical tools: ultraviolet-visible (UV-vis), dynamic light scattering, zeta potential, transmission electron microscope, X-ray diffraction (XRD), and Fourier transform infrared spectroscopy were employed.
31432793	13	9	used	used	1487:1490	arg2	NP					1477:1478	the biogenic Pt NP	1461:1478	the biogenic Pt NP	1461:1478	Author envisages that the biogenic Pt NP can be used in a range of biological and environmental applications.
31432793	0	10	theme	PCR	96:98	arg1	enhancing					100:108	PCR enhancing and antioxidant activities	96:135	enhancing	100:108	Multifaceted activities of plant gum synthesised platinum nanoparticles: catalytic, peroxidase, PCR enhancing and antioxidant activities.
31432793	0	10	theme	PCR	96:98	arg1	nanoparticles					58:70	platinum nanoparticles	49:70	platinum nanoparticles	49:70	Multifaceted activities of plant gum synthesised platinum nanoparticles: catalytic, peroxidase, PCR enhancing and antioxidant activities.
31432793	8	11	theme	5'-tetramethylbenzidine	989:1011	arg1	oxidation					1013:1021	3, 3', 5, 5'-tetramethylbenzidine oxidation	979:1021	3, 3', 5, 5'-tetramethylbenzidine oxidation	979:1021	NP displayed considerable peroxidase activity via catalysis of 3, 3', 5, 5'-tetramethylbenzidine oxidation by H2O2.
31432793	3	12	theme	UV-vis	487:492	arg1	absorption					494:503	Wide continuous UV-vis absorption	471:503	Wide continuous UV-vis absorption	471:503	Wide continuous UV-vis absorption and black solution colouration proved Pt NP formation.
31432793	2	13	theme	Analytical	263:272	arg1	tools					274:278	Analytical tools	263:278	Analytical tools: ultraviolet-visible (UV-vis), dynamic light scattering, zeta potential, transmission electron microscope, X-ray diffraction (XRD), and Fourier transform infrared spectroscopy were employed.	263:469	Analytical tools: ultraviolet-visible (UV-vis), dynamic light scattering, zeta potential, transmission electron microscope, X-ray diffraction (XRD), and Fourier transform infrared spectroscopy were employed.
31432793	3	14	theme	Pt	543:544	arg1	formation					549:557	Pt NP formation	543:557	Pt NP formation	543:557	Wide continuous UV-vis absorption and black solution colouration proved Pt NP formation.
31432793	4	15	theme	crystalline	579:589	arg1	structure					591:599	Face-centred cubic crystalline structure	560:599	Face-centred cubic crystalline structure of NP	560:605	Face-centred cubic crystalline structure of NP was evidenced from XRD.
31432793	13	16	theme	biogenic	1465:1472	arg1	NP					1477:1478	the biogenic Pt NP	1461:1478	the biogenic Pt NP	1461:1478	Author envisages that the biogenic Pt NP can be used in a range of biological and environmental applications.
31432793	7	17	theme	Catalytic	841:849	arg1	action					851:856	Catalytic action	841:856	Catalytic action of NP	841:862	Catalytic action of NP was probed via NaBH4 reduction of arsenazo-III dye.
31432793	10	18	theme	artificial	1254:1263	arg1	peroxidase					1265:1274	an artificial peroxidase	1251:1274	an artificial peroxidase	1251:1274	In comparison with horse radish peroxidase, its applicability as an artificial peroxidase is advantageous.
31432793	6	19	theme	chain	782:786	arg1	PCR					798:800	PCR	798:800	PCR	798:800	NP demonstrated a myriad of properties including catalytic, peroxidase, polymerase chain reaction (PCR) enhancing and antioxidant activities.
31432793	6	19	theme	chain	782:786	arg1	reaction					788:795	polymerase chain reaction	771:795	polymerase chain reaction (PCR)	771:801	NP demonstrated a myriad of properties including catalytic, peroxidase, polymerase chain reaction (PCR) enhancing and antioxidant activities.
31432793	9	20	theme	storage	1149:1155	arg1	duration					1162:1169	storage time duration	1149:1169	storage time duration (0-12 months)	1149:1183	NP showed exceptional stability towards varying pH (3-11), temperature (25-100°C), salt concentration (0-100 mM) and storage time duration (0-12 months).
31432793	9	20	theme	storage	1149:1155	arg1	months					1177:1182	0-12 months	1172:1182	0-12 months	1172:1182	NP showed exceptional stability towards varying pH (3-11), temperature (25-100°C), salt concentration (0-100 mM) and storage time duration (0-12 months).
31432793	10	21	theme	radish	1211:1216	arg1	peroxidase					1218:1227	horse radish peroxidase	1205:1227	horse radish peroxidase	1205:1227	In comparison with horse radish peroxidase, its applicability as an artificial peroxidase is advantageous.
31432793	4	22	theme	cubic	573:577	arg1	structure					591:599	Face-centred cubic crystalline structure	560:599	Face-centred cubic crystalline structure of NP	560:605	Face-centred cubic crystalline structure of NP was evidenced from XRD.
31432793	2	23	theme	X-ray	387:391	arg1	XRD					406:408	XRD	406:408	XRD	406:408	Analytical tools: ultraviolet-visible (UV-vis), dynamic light scattering, zeta potential, transmission electron microscope, X-ray diffraction (XRD), and Fourier transform infrared spectroscopy were employed.
31432793	2	23	theme	X-ray	387:391	arg1	diffraction					393:403	X-ray diffraction	387:403	X-ray diffraction (XRD)	387:409	Analytical tools: ultraviolet-visible (UV-vis), dynamic light scattering, zeta potential, transmission electron microscope, X-ray diffraction (XRD), and Fourier transform infrared spectroscopy were employed.
31432793	5	24	with	spherical	654:662	arg1	size					685:688	a mean particle size	669:688	a mean particle size of 3 nm	669:696	NPs formed were nearly spherical with a mean particle size of 3 nm.
31432793	2	25	theme	electron	366:373	arg1	microscope					375:384	transmission electron microscope	353:384	transmission electron microscope	353:384	Analytical tools: ultraviolet-visible (UV-vis), dynamic light scattering, zeta potential, transmission electron microscope, X-ray diffraction (XRD), and Fourier transform infrared spectroscopy were employed.
31432793	11	26	theme	PCR	1329:1331	arg1	yield					1333:1337	PCR yield	1329:1337	PCR yield	1329:1337	NP caused a two-fold enhancement in PCR yield at 0.4 nM.
31432793	4	27	theme	Face-centred	560:571	arg1	structure					591:599	Face-centred cubic crystalline structure	560:599	Face-centred cubic crystalline structure of NP	560:605	Face-centred cubic crystalline structure of NP was evidenced from XRD.
31432793	7	28	theme	dye	911:913	arg1	reduction					885:893	NaBH4 reduction	879:893	NaBH4 reduction of arsenazo-III dye	879:913	Catalytic action of NP was probed via NaBH4 reduction of arsenazo-III dye.
31432793	3	29	theme	black	509:513	arg1	colouration					524:534	black solution colouration	509:534	black solution colouration	509:534	Wide continuous UV-vis absorption and black solution colouration proved Pt NP formation.
31432793	9	30	theme	exceptional	1042:1052	arg1	stability					1054:1062	exceptional stability	1042:1062	exceptional stability towards varying pH (3-11), temperature (25-100°C), salt concentration (0-100 mM) and storage time duration (0-12 months)	1042:1183	NP showed exceptional stability towards varying pH (3-11), temperature (25-100°C), salt concentration (0-100 mM) and storage time duration (0-12 months).
31432793	2	31	theme	transmission	353:364	arg1	microscope					375:384	transmission electron microscope	353:384	transmission electron microscope	353:384	Analytical tools: ultraviolet-visible (UV-vis), dynamic light scattering, zeta potential, transmission electron microscope, X-ray diffraction (XRD), and Fourier transform infrared spectroscopy were employed.
31432793	8	32	theme	3	979:979	arg1	oxidation					1013:1021	3, 3', 5, 5'-tetramethylbenzidine oxidation	979:1021	3, 3', 5, 5'-tetramethylbenzidine oxidation	979:1021	NP displayed considerable peroxidase activity via catalysis of 3, 3', 5, 5'-tetramethylbenzidine oxidation by H2O2.
31432793	3	33	theme	NP	546:547	arg1	formation					549:557	Pt NP formation	543:557	Pt NP formation	543:557	Wide continuous UV-vis absorption and black solution colouration proved Pt NP formation.
31432793	1	34	theme	platinum	169:176	arg1	nanoparticles					178:190	platinum nanoparticles	169:190	platinum nanoparticles (Pt NP) production	169:209	A single pot, green method for platinum nanoparticles (Pt NP) production was devised with gum ghatti (Anogeissus latifolia).
31432793	1	34	theme	platinum	169:176	arg1	NP					196:197	Pt NP	193:197	Pt NP	193:197	A single pot, green method for platinum nanoparticles (Pt NP) production was devised with gum ghatti (Anogeissus latifolia).
31432793	0	35	theme	Multifaceted	0:11	arg1	activities					13:22	Multifaceted activities	0:22	Multifaceted activities of plant gum	0:35	Multifaceted activities of plant gum synthesised platinum nanoparticles: catalytic, peroxidase, PCR enhancing and antioxidant activities.
31432793	12	36	theme	diphenyl	1381:1388	arg1	scavenging					1407:1416	1' diphenyl picryl-hydrazyle scavenging	1378:1416	1' diphenyl picryl-hydrazyle scavenging (80.1%)	1378:1424	Also showed significant 1', 1' diphenyl picryl-hydrazyle scavenging (80.1%) at 15 µg/mL.
31432793	12	36	theme	diphenyl	1381:1388	arg1	%					1423:1423	80.1%	1419:1423	80.1%	1419:1423	Also showed significant 1', 1' diphenyl picryl-hydrazyle scavenging (80.1%) at 15 µg/mL.
31432793	13	37	theme	Pt	1474:1475	arg1	NP					1477:1478	the biogenic Pt NP	1461:1478	the biogenic Pt NP	1461:1478	Author envisages that the biogenic Pt NP can be used in a range of biological and environmental applications.
31432793	1	38	theme	nanoparticles	178:190	arg1	production					200:209	platinum nanoparticles (Pt NP) production	169:209	platinum nanoparticles (Pt NP) production	169:209	A single pot, green method for platinum nanoparticles (Pt NP) production was devised with gum ghatti (Anogeissus latifolia).
31432793	0	39	theme	plant	27:31	arg1	gum					33:35	plant gum	27:35	plant gum	27:35	Multifaceted activities of plant gum synthesised platinum nanoparticles: catalytic, peroxidase, PCR enhancing and antioxidant activities.
31432793	10	40	theme	horse	1205:1209	arg1	peroxidase					1218:1227	horse radish peroxidase	1205:1227	horse radish peroxidase	1205:1227	In comparison with horse radish peroxidase, its applicability as an artificial peroxidase is advantageous.
31432793	5	41	theme	particle	676:683	arg1	size					685:688	a mean particle size	669:688	a mean particle size of 3 nm	669:696	NPs formed were nearly spherical with a mean particle size of 3 nm.
31432793	6	42	theme	polymerase	771:780	arg1	PCR					798:800	PCR	798:800	PCR	798:800	NP demonstrated a myriad of properties including catalytic, peroxidase, polymerase chain reaction (PCR) enhancing and antioxidant activities.
31432793	6	42	theme	polymerase	771:780	arg1	reaction					788:795	polymerase chain reaction	771:795	polymerase chain reaction (PCR)	771:801	NP demonstrated a myriad of properties including catalytic, peroxidase, polymerase chain reaction (PCR) enhancing and antioxidant activities.
31432793	10	43	from	advantageous	1279:1290	arg1	comparison					1189:1198	comparison	1189:1198	comparison with horse radish peroxidase	1189:1227	In comparison with horse radish peroxidase, its applicability as an artificial peroxidase is advantageous.
31432793	0	44	theme	antioxidant	114:124	arg1	activities					126:135	PCR enhancing and antioxidant activities	96:135	activities	126:135	Multifaceted activities of plant gum synthesised platinum nanoparticles: catalytic, peroxidase, PCR enhancing and antioxidant activities.
31432793	0	44	theme	antioxidant	114:124	arg1	nanoparticles					58:70	platinum nanoparticles	49:70	platinum nanoparticles	49:70	Multifaceted activities of plant gum synthesised platinum nanoparticles: catalytic, peroxidase, PCR enhancing and antioxidant activities.
31432793	9	45	theme	time	1157:1160	arg1	duration					1162:1169	storage time duration	1149:1169	storage time duration (0-12 months)	1149:1183	NP showed exceptional stability towards varying pH (3-11), temperature (25-100°C), salt concentration (0-100 mM) and storage time duration (0-12 months).
31432793	9	45	theme	time	1157:1160	arg1	months					1177:1182	0-12 months	1172:1182	0-12 months	1172:1182	NP showed exceptional stability towards varying pH (3-11), temperature (25-100°C), salt concentration (0-100 mM) and storage time duration (0-12 months).
31432793	1	46	theme	green	152:156	arg1	method					158:163	A single pot, green method	138:163	method	158:163	A single pot, green method for platinum nanoparticles (Pt NP) production was devised with gum ghatti (Anogeissus latifolia).
31432793	1	47	theme	Pt	193:194	arg1	nanoparticles					178:190	platinum nanoparticles	169:190	platinum nanoparticles (Pt NP) production	169:209	A single pot, green method for platinum nanoparticles (Pt NP) production was devised with gum ghatti (Anogeissus latifolia).
31432793	1	47	theme	Pt	193:194	arg1	NP					196:197	Pt NP	193:197	Pt NP	193:197	A single pot, green method for platinum nanoparticles (Pt NP) production was devised with gum ghatti (Anogeissus latifolia).
31432793	2	48	theme	infrared	434:441	arg1	spectroscopy					443:454	infrared spectroscopy	434:454	infrared spectroscopy	434:454	Analytical tools: ultraviolet-visible (UV-vis), dynamic light scattering, zeta potential, transmission electron microscope, X-ray diffraction (XRD), and Fourier transform infrared spectroscopy were employed.
31432793	6	49	dep	catalytic	748:756	arg1	activities					829:838	activities	829:838	activities	829:838	NP demonstrated a myriad of properties including catalytic, peroxidase, polymerase chain reaction (PCR) enhancing and antioxidant activities.
31432793	0	50	dep	nanoparticles	58:70	arg1	catalytic					73:81	catalytic	73:81	catalytic	73:81	Multifaceted activities of plant gum synthesised platinum nanoparticles: catalytic, peroxidase, PCR enhancing and antioxidant activities.
31432793	0	51	theme	gum	33:35	arg1	activities					13:22	Multifaceted activities	0:22	Multifaceted activities of plant gum	0:35	Multifaceted activities of plant gum synthesised platinum nanoparticles: catalytic, peroxidase, PCR enhancing and antioxidant activities.
31432793	5	52	theme	3 nm	693:696	arg1	size					685:688	a mean particle size	669:688	a mean particle size of 3 nm	669:696	NPs formed were nearly spherical with a mean particle size of 3 nm.
31432793	6	53	theme	properties	727:736	arg1	myriad					717:722	a myriad	715:722	a myriad of properties including catalytic, peroxidase, polymerase chain reaction (PCR) enhancing and antioxidant activities	715:838	NP demonstrated a myriad of properties including catalytic, peroxidase, polymerase chain reaction (PCR) enhancing and antioxidant activities.
31432793	2	54	theme	dynamic	311:317	arg1	scattering					325:334	dynamic light scattering	311:334	dynamic light scattering	311:334	Analytical tools: ultraviolet-visible (UV-vis), dynamic light scattering, zeta potential, transmission electron microscope, X-ray diffraction (XRD), and Fourier transform infrared spectroscopy were employed.
31432793	0	55	theme	platinum	49:56	arg1	peroxidase					84:93	peroxidase	84:93	peroxidase	84:93	Multifaceted activities of plant gum synthesised platinum nanoparticles: catalytic, peroxidase, PCR enhancing and antioxidant activities.
31432793	0	55	theme	platinum	49:56	arg1	enhancing					100:108	PCR enhancing and antioxidant activities	96:135	enhancing	100:108	Multifaceted activities of plant gum synthesised platinum nanoparticles: catalytic, peroxidase, PCR enhancing and antioxidant activities.
31432793	0	55	theme	platinum	49:56	arg1	activities					126:135	PCR enhancing and antioxidant activities	96:135	activities	126:135	Multifaceted activities of plant gum synthesised platinum nanoparticles: catalytic, peroxidase, PCR enhancing and antioxidant activities.
31432793	0	55	theme	platinum	49:56	arg1	nanoparticles					58:70	platinum nanoparticles	49:70	platinum nanoparticles	49:70	Multifaceted activities of plant gum synthesised platinum nanoparticles: catalytic, peroxidase, PCR enhancing and antioxidant activities.
31432793	13	56	theme	environmental	1521:1533	arg1	applications					1535:1546	biological and environmental applications	1506:1546	biological and environmental applications	1506:1546	Author envisages that the biogenic Pt NP can be used in a range of biological and environmental applications.
31432793	8	57	theme	oxidation	1013:1021	arg1	catalysis					966:974	catalysis	966:974	catalysis of 3, 3', 5, 5'-tetramethylbenzidine oxidation by H2O2	966:1029	NP displayed considerable peroxidase activity via catalysis of 3, 3', 5, 5'-tetramethylbenzidine oxidation by H2O2.
31432793	3	58	theme	continuous	476:485	arg1	absorption					494:503	Wide continuous UV-vis absorption	471:503	Wide continuous UV-vis absorption	471:503	Wide continuous UV-vis absorption and black solution colouration proved Pt NP formation.
31432793	2	59	dep	tools	274:278	arg1	employed					461:468	employed	461:468	were employed	456:468	Analytical tools: ultraviolet-visible (UV-vis), dynamic light scattering, zeta potential, transmission electron microscope, X-ray diffraction (XRD), and Fourier transform infrared spectroscopy were employed.
31432793	7	60	theme	NaBH4	879:883	arg1	reduction					885:893	NaBH4 reduction	879:893	NaBH4 reduction of arsenazo-III dye	879:913	Catalytic action of NP was probed via NaBH4 reduction of arsenazo-III dye.
31432793	7	61	theme	arsenazo-III	898:909	arg1	dye					911:913	arsenazo-III dye	898:913	arsenazo-III dye	898:913	Catalytic action of NP was probed via NaBH4 reduction of arsenazo-III dye.
31432793	13	62	theme	biological	1506:1515	arg1	applications					1535:1546	biological and environmental applications	1506:1546	biological and environmental applications	1506:1546	Author envisages that the biogenic Pt NP can be used in a range of biological and environmental applications.
31432793	1	63	theme	single	140:145	arg1	method					158:163	A single pot, green method	138:163	method	158:163	A single pot, green method for platinum nanoparticles (Pt NP) production was devised with gum ghatti (Anogeissus latifolia).
31432793	5	64	theme	mean	671:674	arg1	size					685:688	a mean particle size	669:688	a mean particle size of 3 nm	669:696	NPs formed were nearly spherical with a mean particle size of 3 nm.
31432793	12	65	theme	picryl-hydrazyle	1390:1405	arg1	scavenging					1407:1416	1' diphenyl picryl-hydrazyle scavenging	1378:1416	1' diphenyl picryl-hydrazyle scavenging (80.1%)	1378:1424	Also showed significant 1', 1' diphenyl picryl-hydrazyle scavenging (80.1%) at 15 µg/mL.
31432793	12	65	theme	picryl-hydrazyle	1390:1405	arg1	%					1423:1423	80.1%	1419:1423	80.1%	1419:1423	Also showed significant 1', 1' diphenyl picryl-hydrazyle scavenging (80.1%) at 15 µg/mL.
31432793	3	66	theme	solution	515:522	arg1	colouration					524:534	black solution colouration	509:534	black solution colouration	509:534	Wide continuous UV-vis absorption and black solution colouration proved Pt NP formation.
31432793	2	67	dep	ultraviolet-visible	281:299	arg1	UV-vis					302:307	UV-vis	302:307	UV-vis	302:307	Analytical tools: ultraviolet-visible (UV-vis), dynamic light scattering, zeta potential, transmission electron microscope, X-ray diffraction (XRD), and Fourier transform infrared spectroscopy were employed.
31432793	11	68	theme	two-fold	1305:1312	arg1	enhancement					1314:1324	a two-fold enhancement	1303:1324	a two-fold enhancement	1303:1324	NP caused a two-fold enhancement in PCR yield at 0.4 nM.
31432793	1	69	dep	ghatti	232:237	arg1	latifolia					251:259	Anogeissus latifolia	240:259	Anogeissus latifolia	240:259	A single pot, green method for platinum nanoparticles (Pt NP) production was devised with gum ghatti (Anogeissus latifolia).
31432793	2	70	theme	zeta	337:340	arg1	potential					342:350	zeta potential	337:350	zeta potential	337:350	Analytical tools: ultraviolet-visible (UV-vis), dynamic light scattering, zeta potential, transmission electron microscope, X-ray diffraction (XRD), and Fourier transform infrared spectroscopy were employed.
31432793	8	71	theme	peroxidase	942:951	arg1	activity					953:960	considerable peroxidase activity	929:960	considerable peroxidase activity	929:960	NP displayed considerable peroxidase activity via catalysis of 3, 3', 5, 5'-tetramethylbenzidine oxidation by H2O2.
31432793	1	72	theme	pot	147:149	arg1	method					158:163	A single pot, green method	138:163	method	158:163	A single pot, green method for platinum nanoparticles (Pt NP) production was devised with gum ghatti (Anogeissus latifolia).
31432793	7	73	theme	NP	861:862	arg1	action					851:856	Catalytic action	841:856	Catalytic action of NP	841:862	Catalytic action of NP was probed via NaBH4 reduction of arsenazo-III dye.
31432793	4	74	theme	NP	604:605	arg1	structure					591:599	Face-centred cubic crystalline structure	560:599	Face-centred cubic crystalline structure of NP	560:605	Face-centred cubic crystalline structure of NP was evidenced from XRD.
29796594	7	0	theme	inter-alpha-trypsin	1196:1214	arg1	chain					1232:1236	inter-alpha-trypsin inhibitor heavy chain	1196:1236	inter-alpha-trypsin inhibitor heavy chain H4	1196:1239	The preferred plasma protein ligands for a sialyl-T antigen-selective SLBR are proteoglycan 4 (lubricin) and inter-alpha-trypsin inhibitor heavy chain H4.
29796594	3	1	theme	platelet	557:564	arg1	GPIbα					566:570	platelet GPIbα	557:570	platelet GPIbα	557:570	Two known receptors for the Siglec-like adhesins are the salivary mucin MG2/MUC7 and platelet GPIbα, and the interaction of streptococci with these targets may contribute to oral colonization and endocarditis, respectively.
29796594	9	2	theme	protease	1515:1522	arg1	cascades					1524:1531	the complement and kallikrein protease cascades	1485:1531	cascades	1524:1531	All four SLBRs recognize C1 inhibitor but detect distinctly different glycoforms of this key regulator of the complement and kallikrein protease cascades.
29796594	11	3	theme	adhesins	1759:1766	arg1	interaction					1732:1742	the interaction	1728:1742	the interaction of Siglec-like adhesins with different subsets of plasma glycoproteins	1728:1813	The combined results suggest that the interaction of Siglec-like adhesins with different subsets of plasma glycoproteins could have a significant impact on the propensity of streptococci to establish endocardial infections.
29796594	2	4	theme	repeat	331:336	arg1	adhesins					338:345	cell surface serine-rich repeat adhesins	306:345	cell surface serine-rich repeat adhesins containing "Siglec-like" binding regions (SLBRs) that mediate attachment to α2-3-linked sialic acids on human glycoproteins	306:469	These organisms express cell surface serine-rich repeat adhesins containing "Siglec-like" binding regions (SLBRs) that mediate attachment to α2-3-linked sialic acids on human glycoproteins.
29796594	2	5	theme	surface	311:317	arg1	adhesins					338:345	cell surface serine-rich repeat adhesins	306:345	cell surface serine-rich repeat adhesins containing "Siglec-like" binding regions (SLBRs) that mediate attachment to α2-3-linked sialic acids on human glycoproteins	306:469	These organisms express cell surface serine-rich repeat adhesins containing "Siglec-like" binding regions (SLBRs) that mediate attachment to α2-3-linked sialic acids on human glycoproteins.
29796594	10	6	theme	other	1678:1682	arg1	diseases					1684:1691	other diseases	1678:1691	other diseases	1678:1691	The four plasma ligands have potential roles in thrombosis and inflammation, and each has been cited as a biomarker for one or more vascular or other diseases.
29796594	2	7	theme	Siglec-like	359:369	arg1	SLBRs					389:393	SLBRs	389:393	SLBRs	389:393	These organisms express cell surface serine-rich repeat adhesins containing "Siglec-like" binding regions (SLBRs) that mediate attachment to α2-3-linked sialic acids on human glycoproteins.
29796594	2	7	theme	Siglec-like	359:369	arg1	regions					380:386	"Siglec-like" binding regions	358:386	"Siglec-like" binding regions (SLBRs) that mediate attachment to α2-3-linked sialic acids on human glycoproteins	358:469	These organisms express cell surface serine-rich repeat adhesins containing "Siglec-like" binding regions (SLBRs) that mediate attachment to α2-3-linked sialic acids on human glycoproteins.
29796594	1	8	theme	oral	223:226	arg1	microbiota					228:237	the normal oral microbiota	212:237	the normal oral microbiota	212:237	Streptococcus gordonii and Streptococcus sanguinis are typically found among the normal oral microbiota but can also cause infective endocarditis.
29796594	8	9	theme	platelet	1362:1369	arg1	GPIbα					1371:1375	platelet GPIbα	1362:1375	platelet GPIbα	1362:1375	Conversely, the preferred ligand for a 3'sialyllactosamine-selective SLBR is glycocalicin (the extracellular portion of platelet GPIbα).
29796594	6	10	theme	small	1018:1022	arg1	subset					1024:1029	a surprisingly small subset	1003:1029	a surprisingly small subset of plasma proteins that are extensively O-glycosylated	1003:1084	We found that the SLBRs recognize a surprisingly small subset of plasma proteins that are extensively O-glycosylated.
29796594	7	11	theme	sialyl-T	1130:1137	arg1	SLBR					1157:1160	a sialyl-T antigen-selective SLBR	1128:1160	a sialyl-T antigen-selective SLBR	1128:1160	The preferred plasma protein ligands for a sialyl-T antigen-selective SLBR are proteoglycan 4 (lubricin) and inter-alpha-trypsin inhibitor heavy chain H4.
29796594	3	12	theme	oral	646:649	arg1	colonization					651:662	oral colonization	646:662	oral colonization	646:662	Two known receptors for the Siglec-like adhesins are the salivary mucin MG2/MUC7 and platelet GPIbα, and the interaction of streptococci with these targets may contribute to oral colonization and endocarditis, respectively.
29796594	10	13	from	roles	1573:1577	arg1	inflammation					1597:1608	inflammation	1597:1608	inflammation	1597:1608	The four plasma ligands have potential roles in thrombosis and inflammation, and each has been cited as a biomarker for one or more vascular or other diseases.
29796594	10	13	from	roles	1573:1577	arg1	thrombosis					1582:1591	thrombosis	1582:1591	thrombosis	1582:1591	The four plasma ligands have potential roles in thrombosis and inflammation, and each has been cited as a biomarker for one or more vascular or other diseases.
29796594	7	14	theme	protein	1108:1114	arg1	ligands					1116:1122	The preferred plasma protein ligands	1087:1122	The preferred plasma protein ligands for a sialyl-T antigen-selective SLBR	1087:1160	The preferred plasma protein ligands for a sialyl-T antigen-selective SLBR are proteoglycan 4 (lubricin) and inter-alpha-trypsin inhibitor heavy chain H4.
29796594	7	14	theme	protein	1108:1114	arg1	proteoglycan					1166:1177	proteoglycan 4	1166:1179	proteoglycan 4 (lubricin)	1166:1190	The preferred plasma protein ligands for a sialyl-T antigen-selective SLBR are proteoglycan 4 (lubricin) and inter-alpha-trypsin inhibitor heavy chain H4.
29796594	4	15	theme	preferences	740:750	arg1	diversity					727:735	a surprising diversity	714:735	a surprising diversity of preferences for defined glycans, ranging from highly selective to broader specificity	714:824	The SLBRs display a surprising diversity of preferences for defined glycans, ranging from highly selective to broader specificity.
29796594	11	16	theme	significant	1828:1838	arg1	impact					1840:1845	a significant impact	1826:1845	a significant impact	1826:1845	The combined results suggest that the interaction of Siglec-like adhesins with different subsets of plasma glycoproteins could have a significant impact on the propensity of streptococci to establish endocardial infections.
29796594	0	17	theme	plasma	72:77	arg1	glycoproteins					79:91	human plasma glycoproteins	66:91	human plasma glycoproteins	66:91	Streptococcal Siglec-like adhesins recognize different subsets of human plasma glycoproteins: implications for infective endocarditis.
29796594	5	18	theme	sialoglycan	945:955	arg1	structures					957:966	different α2-3 sialoglycan structures	930:966	different α2-3 sialoglycan structures	930:966	In this report, we characterize the glycoproteins in human plasma recognized by four SLBRs that prefer different α2-3 sialoglycan structures.
29796594	2	19	gly	glycoproteins	457:469	arg1	glycoproteins					457:469	human glycoproteins	451:469	human glycoproteins	451:469	These organisms express cell surface serine-rich repeat adhesins containing "Siglec-like" binding regions (SLBRs) that mediate attachment to α2-3-linked sialic acids on human glycoproteins.
29796594	4	20	theme	defined	756:762	arg1	glycans					764:770	defined glycans	756:770	defined glycans	756:770	The SLBRs display a surprising diversity of preferences for defined glycans, ranging from highly selective to broader specificity.
29796594	11	21	theme	streptococci	1868:1879	arg1	propensity					1854:1863	the propensity	1850:1863	the propensity of streptococci to establish endocardial infections	1850:1915	The combined results suggest that the interaction of Siglec-like adhesins with different subsets of plasma glycoproteins could have a significant impact on the propensity of streptococci to establish endocardial infections.
29796594	0	22	gly	glycoproteins	79:91	arg1	glycoproteins					79:91	human plasma glycoproteins	66:91	human plasma glycoproteins	66:91	Streptococcal Siglec-like adhesins recognize different subsets of human plasma glycoproteins: implications for infective endocarditis.
29796594	6	23	theme	proteins	1041:1048	arg1	subset					1024:1029	a surprisingly small subset	1003:1029	a surprisingly small subset of plasma proteins that are extensively O-glycosylated	1003:1084	We found that the SLBRs recognize a surprisingly small subset of plasma proteins that are extensively O-glycosylated.
29796594	9	24	gly	glycoforms	1449:1458	arg1	regulator					1472:1480	this key regulator	1463:1480	this key regulator of the complement and kallikrein protease cascades	1463:1531	All four SLBRs recognize C1 inhibitor but detect distinctly different glycoforms of this key regulator of the complement and kallikrein protease cascades.
29796594	10	25	theme	potential	1563:1571	arg1	roles					1573:1577	potential roles	1563:1577	potential roles in thrombosis and inflammation	1563:1608	The four plasma ligands have potential roles in thrombosis and inflammation, and each has been cited as a biomarker for one or more vascular or other diseases.
29796594	2	26	theme	α2-3-linked	423:433	arg1	acids					442:446	α2-3-linked sialic acids	423:446	α2-3-linked sialic acids	423:446	These organisms express cell surface serine-rich repeat adhesins containing "Siglec-like" binding regions (SLBRs) that mediate attachment to α2-3-linked sialic acids on human glycoproteins.
29796594	0	27	theme	Streptococcal	0:12	arg1	adhesins					26:33	Streptococcal Siglec-like adhesins	0:33	Streptococcal Siglec-like adhesins	0:33	Streptococcal Siglec-like adhesins recognize different subsets of human plasma glycoproteins: implications for infective endocarditis.
29796594	8	28	dep	glycocalicin	1319:1330	arg1	portion					1351:1357	the extracellular portion	1333:1357	the extracellular portion of platelet GPIbα	1333:1375	Conversely, the preferred ligand for a 3'sialyllactosamine-selective SLBR is glycocalicin (the extracellular portion of platelet GPIbα).
29796594	4	29	theme	broader	806:812	arg1	specificity					814:824	broader specificity	806:824	broader specificity	806:824	The SLBRs display a surprising diversity of preferences for defined glycans, ranging from highly selective to broader specificity.
29796594	5	30	theme	human	880:884	arg1	plasma					886:891	human plasma	880:891	human plasma recognized by four SLBRs that prefer different α2-3 sialoglycan structures	880:966	In this report, we characterize the glycoproteins in human plasma recognized by four SLBRs that prefer different α2-3 sialoglycan structures.
29796594	9	31	theme	different	1439:1447	arg1	glycoforms					1449:1458	distinctly different glycoforms	1428:1458	distinctly different glycoforms of this key regulator of the complement and kallikrein protease cascades	1428:1531	All four SLBRs recognize C1 inhibitor but detect distinctly different glycoforms of this key regulator of the complement and kallikrein protease cascades.
29796594	11	32	theme	plasma	1794:1799	arg1	glycoproteins					1801:1813	plasma glycoproteins	1794:1813	plasma glycoproteins	1794:1813	The combined results suggest that the interaction of Siglec-like adhesins with different subsets of plasma glycoproteins could have a significant impact on the propensity of streptococci to establish endocardial infections.
29796594	5	33	gly	glycoproteins	863:875	arg1	glycoproteins					863:875	the glycoproteins	859:875	the glycoproteins in human plasma recognized by four SLBRs that prefer different α2-3 sialoglycan structures	859:966	In this report, we characterize the glycoproteins in human plasma recognized by four SLBRs that prefer different α2-3 sialoglycan structures.
29796594	2	34	theme	human	451:455	arg1	glycoproteins					457:469	human glycoproteins	451:469	human glycoproteins	451:469	These organisms express cell surface serine-rich repeat adhesins containing "Siglec-like" binding regions (SLBRs) that mediate attachment to α2-3-linked sialic acids on human glycoproteins.
29796594	0	35	theme	different	45:53	arg1	subsets					55:61	different subsets	45:61	different subsets of human plasma glycoproteins: implications for infective endocarditis	45:132	Streptococcal Siglec-like adhesins recognize different subsets of human plasma glycoproteins: implications for infective endocarditis.
29796594	2	36	contain	containing	347:356	arg1	adhesins					338:345	cell surface serine-rich repeat adhesins	306:345	cell surface serine-rich repeat adhesins containing "Siglec-like" binding regions (SLBRs) that mediate attachment to α2-3-linked sialic acids on human glycoproteins	306:469	These organisms express cell surface serine-rich repeat adhesins containing "Siglec-like" binding regions (SLBRs) that mediate attachment to α2-3-linked sialic acids on human glycoproteins.
29796594	2	36	contain	containing	347:356	arg2	SLBRs					389:393	SLBRs	389:393	SLBRs	389:393	These organisms express cell surface serine-rich repeat adhesins containing "Siglec-like" binding regions (SLBRs) that mediate attachment to α2-3-linked sialic acids on human glycoproteins.
29796594	2	36	contain	containing	347:356	arg2	regions					380:386	"Siglec-like" binding regions	358:386	"Siglec-like" binding regions (SLBRs) that mediate attachment to α2-3-linked sialic acids on human glycoproteins	358:469	These organisms express cell surface serine-rich repeat adhesins containing "Siglec-like" binding regions (SLBRs) that mediate attachment to α2-3-linked sialic acids on human glycoproteins.
29796594	3	37	theme	mucin	538:542	arg1	receptors					482:490	Two known receptors	472:490	Two known receptors for the Siglec-like adhesins	472:519	Two known receptors for the Siglec-like adhesins are the salivary mucin MG2/MUC7 and platelet GPIbα, and the interaction of streptococci with these targets may contribute to oral colonization and endocarditis, respectively.
29796594	3	37	theme	mucin	538:542	arg1	MG2/MUC7					544:551	the salivary mucin MG2/MUC7	525:551	the salivary mucin MG2/MUC7	525:551	Two known receptors for the Siglec-like adhesins are the salivary mucin MG2/MUC7 and platelet GPIbα, and the interaction of streptococci with these targets may contribute to oral colonization and endocarditis, respectively.
29796594	7	38	theme	heavy	1226:1230	arg1	chain					1232:1236	inter-alpha-trypsin inhibitor heavy chain	1196:1236	inter-alpha-trypsin inhibitor heavy chain H4	1196:1239	The preferred plasma protein ligands for a sialyl-T antigen-selective SLBR are proteoglycan 4 (lubricin) and inter-alpha-trypsin inhibitor heavy chain H4.
29796594	9	39	theme	key	1468:1470	arg1	regulator					1472:1480	this key regulator	1463:1480	this key regulator of the complement and kallikrein protease cascades	1463:1531	All four SLBRs recognize C1 inhibitor but detect distinctly different glycoforms of this key regulator of the complement and kallikrein protease cascades.
29796594	9	40	theme	complement	1489:1498	arg1	regulator					1472:1480	this key regulator	1463:1480	this key regulator of the complement and kallikrein protease cascades	1463:1531	All four SLBRs recognize C1 inhibitor but detect distinctly different glycoforms of this key regulator of the complement and kallikrein protease cascades.
29796594	0	41	theme	human	66:70	arg1	glycoproteins					79:91	human plasma glycoproteins	66:91	human plasma glycoproteins	66:91	Streptococcal Siglec-like adhesins recognize different subsets of human plasma glycoproteins: implications for infective endocarditis.
29796594	9	42	theme	kallikrein	1504:1513	arg1	cascades					1524:1531	the complement and kallikrein protease cascades	1485:1531	cascades	1524:1531	All four SLBRs recognize C1 inhibitor but detect distinctly different glycoforms of this key regulator of the complement and kallikrein protease cascades.
29796594	11	43	with	interaction	1732:1742	arg1	subsets					1783:1789	different subsets	1773:1789	different subsets of plasma glycoproteins	1773:1813	The combined results suggest that the interaction of Siglec-like adhesins with different subsets of plasma glycoproteins could have a significant impact on the propensity of streptococci to establish endocardial infections.
29796594	9	44	theme	cascades	1524:1531	arg1	regulator					1472:1480	this key regulator	1463:1480	this key regulator of the complement and kallikrein protease cascades	1463:1531	All four SLBRs recognize C1 inhibitor but detect distinctly different glycoforms of this key regulator of the complement and kallikrein protease cascades.
29796594	2	45	theme	serine-rich	319:329	arg1	adhesins					338:345	cell surface serine-rich repeat adhesins	306:345	cell surface serine-rich repeat adhesins containing "Siglec-like" binding regions (SLBRs) that mediate attachment to α2-3-linked sialic acids on human glycoproteins	306:469	These organisms express cell surface serine-rich repeat adhesins containing "Siglec-like" binding regions (SLBRs) that mediate attachment to α2-3-linked sialic acids on human glycoproteins.
29796594	11	46	theme	Siglec-like	1747:1757	arg1	adhesins					1759:1766	Siglec-like adhesins	1747:1766	Siglec-like adhesins	1747:1766	The combined results suggest that the interaction of Siglec-like adhesins with different subsets of plasma glycoproteins could have a significant impact on the propensity of streptococci to establish endocardial infections.
29796594	2	47	theme	"	370:370	arg1	SLBRs					389:393	SLBRs	389:393	SLBRs	389:393	These organisms express cell surface serine-rich repeat adhesins containing "Siglec-like" binding regions (SLBRs) that mediate attachment to α2-3-linked sialic acids on human glycoproteins.
29796594	2	47	theme	"	370:370	arg1	regions					380:386	"Siglec-like" binding regions	358:386	"Siglec-like" binding regions (SLBRs) that mediate attachment to α2-3-linked sialic acids on human glycoproteins	358:469	These organisms express cell surface serine-rich repeat adhesins containing "Siglec-like" binding regions (SLBRs) that mediate attachment to α2-3-linked sialic acids on human glycoproteins.
29796594	1	48	theme	normal	216:221	arg1	microbiota					228:237	the normal oral microbiota	212:237	the normal oral microbiota	212:237	Streptococcus gordonii and Streptococcus sanguinis are typically found among the normal oral microbiota but can also cause infective endocarditis.
29796594	8	49	theme	preferred	1258:1266	arg1	ligand					1268:1273	the preferred ligand	1254:1273	the preferred ligand for a 3'sialyllactosamine-selective SLBR	1254:1314	Conversely, the preferred ligand for a 3'sialyllactosamine-selective SLBR is glycocalicin (the extracellular portion of platelet GPIbα).
29796594	8	49	theme	preferred	1258:1266	arg1	glycocalicin					1319:1330	glycocalicin	1319:1330	glycocalicin (the extracellular portion of platelet GPIbα)	1319:1376	Conversely, the preferred ligand for a 3'sialyllactosamine-selective SLBR is glycocalicin (the extracellular portion of platelet GPIbα).
29796594	6	50	theme	plasma	1034:1039	arg1	proteins					1041:1048	plasma proteins	1034:1048	plasma proteins that are extensively O-glycosylated	1034:1084	We found that the SLBRs recognize a surprisingly small subset of plasma proteins that are extensively O-glycosylated.
29796594	2	51	from	attachment	409:418	arg1	glycoproteins					457:469	human glycoproteins	451:469	human glycoproteins	451:469	These organisms express cell surface serine-rich repeat adhesins containing "Siglec-like" binding regions (SLBRs) that mediate attachment to α2-3-linked sialic acids on human glycoproteins.
29796594	8	52	theme	extracellular	1337:1349	arg1	portion					1351:1357	the extracellular portion	1333:1357	the extracellular portion of platelet GPIbα	1333:1375	Conversely, the preferred ligand for a 3'sialyllactosamine-selective SLBR is glycocalicin (the extracellular portion of platelet GPIbα).
29796594	7	53	theme	antigen-selective	1139:1155	arg1	SLBR					1157:1160	a sialyl-T antigen-selective SLBR	1128:1160	a sialyl-T antigen-selective SLBR	1128:1160	The preferred plasma protein ligands for a sialyl-T antigen-selective SLBR are proteoglycan 4 (lubricin) and inter-alpha-trypsin inhibitor heavy chain H4.
29796594	3	54	theme	streptococci	596:607	arg1	interaction					581:591	the interaction	577:591	the interaction of streptococci with these targets	577:626	Two known receptors for the Siglec-like adhesins are the salivary mucin MG2/MUC7 and platelet GPIbα, and the interaction of streptococci with these targets may contribute to oral colonization and endocarditis, respectively.
29796594	10	55	contain	have	1558:1561	arg1	ligands					1550:1556	The four plasma ligands	1534:1556	The four plasma ligands	1534:1556	The four plasma ligands have potential roles in thrombosis and inflammation, and each has been cited as a biomarker for one or more vascular or other diseases.
29796594	10	55	contain	have	1558:1561	arg2	roles					1573:1577	potential roles	1563:1577	potential roles in thrombosis and inflammation	1563:1608	The four plasma ligands have potential roles in thrombosis and inflammation, and each has been cited as a biomarker for one or more vascular or other diseases.
29796594	11	56	theme	endocardial	1894:1904	arg1	infections					1906:1915	endocardial infections	1894:1915	endocardial infections	1894:1915	The combined results suggest that the interaction of Siglec-like adhesins with different subsets of plasma glycoproteins could have a significant impact on the propensity of streptococci to establish endocardial infections.
29796594	4	57	theme	surprising	716:725	arg1	diversity					727:735	a surprising diversity	714:735	a surprising diversity of preferences for defined glycans, ranging from highly selective to broader specificity	714:824	The SLBRs display a surprising diversity of preferences for defined glycans, ranging from highly selective to broader specificity.
29796594	8	58	theme	3'sialyllactosamine-selective	1281:1309	arg1	SLBR					1311:1314	a 3'sialyllactosamine-selective SLBR	1279:1314	a 3'sialyllactosamine-selective SLBR	1279:1314	Conversely, the preferred ligand for a 3'sialyllactosamine-selective SLBR is glycocalicin (the extracellular portion of platelet GPIbα).
29796594	8	59	theme	GPIbα	1371:1375	arg1	portion					1351:1357	the extracellular portion	1333:1357	the extracellular portion of platelet GPIbα	1333:1375	Conversely, the preferred ligand for a 3'sialyllactosamine-selective SLBR is glycocalicin (the extracellular portion of platelet GPIbα).
29796594	7	60	theme	preferred	1091:1099	arg1	ligands					1116:1122	The preferred plasma protein ligands	1087:1122	The preferred plasma protein ligands for a sialyl-T antigen-selective SLBR	1087:1160	The preferred plasma protein ligands for a sialyl-T antigen-selective SLBR are proteoglycan 4 (lubricin) and inter-alpha-trypsin inhibitor heavy chain H4.
29796594	7	60	theme	preferred	1091:1099	arg1	proteoglycan					1166:1177	proteoglycan 4	1166:1179	proteoglycan 4 (lubricin)	1166:1190	The preferred plasma protein ligands for a sialyl-T antigen-selective SLBR are proteoglycan 4 (lubricin) and inter-alpha-trypsin inhibitor heavy chain H4.
29796594	7	61	theme	plasma	1101:1106	arg1	ligands					1116:1122	The preferred plasma protein ligands	1087:1122	The preferred plasma protein ligands for a sialyl-T antigen-selective SLBR	1087:1160	The preferred plasma protein ligands for a sialyl-T antigen-selective SLBR are proteoglycan 4 (lubricin) and inter-alpha-trypsin inhibitor heavy chain H4.
29796594	7	61	theme	plasma	1101:1106	arg1	proteoglycan					1166:1177	proteoglycan 4	1166:1179	proteoglycan 4 (lubricin)	1166:1190	The preferred plasma protein ligands for a sialyl-T antigen-selective SLBR are proteoglycan 4 (lubricin) and inter-alpha-trypsin inhibitor heavy chain H4.
29796594	2	62	theme	cell	306:309	arg1	adhesins					338:345	cell surface serine-rich repeat adhesins	306:345	cell surface serine-rich repeat adhesins containing "Siglec-like" binding regions (SLBRs) that mediate attachment to α2-3-linked sialic acids on human glycoproteins	306:469	These organisms express cell surface serine-rich repeat adhesins containing "Siglec-like" binding regions (SLBRs) that mediate attachment to α2-3-linked sialic acids on human glycoproteins.
29796594	5	63	theme	different	930:938	arg1	structures					957:966	different α2-3 sialoglycan structures	930:966	different α2-3 sialoglycan structures	930:966	In this report, we characterize the glycoproteins in human plasma recognized by four SLBRs that prefer different α2-3 sialoglycan structures.
29796594	11	64	gly	glycoproteins	1801:1813	arg1	glycoproteins					1801:1813	plasma glycoproteins	1794:1813	plasma glycoproteins	1794:1813	The combined results suggest that the interaction of Siglec-like adhesins with different subsets of plasma glycoproteins could have a significant impact on the propensity of streptococci to establish endocardial infections.
29796594	0	65	theme	glycoproteins	79:91	arg1	subsets					55:61	different subsets	45:61	different subsets of human plasma glycoproteins: implications for infective endocarditis	45:132	Streptococcal Siglec-like adhesins recognize different subsets of human plasma glycoproteins: implications for infective endocarditis.
29796594	5	66	theme	α2-3	940:943	arg1	structures					957:966	different α2-3 sialoglycan structures	930:966	different α2-3 sialoglycan structures	930:966	In this report, we characterize the glycoproteins in human plasma recognized by four SLBRs that prefer different α2-3 sialoglycan structures.
29796594	11	67	contain	have	1821:1824	arg1	interaction					1732:1742	the interaction	1728:1742	the interaction of Siglec-like adhesins with different subsets of plasma glycoproteins	1728:1813	The combined results suggest that the interaction of Siglec-like adhesins with different subsets of plasma glycoproteins could have a significant impact on the propensity of streptococci to establish endocardial infections.
29796594	11	67	contain	have	1821:1824	arg2	impact					1840:1845	a significant impact	1826:1845	a significant impact	1826:1845	The combined results suggest that the interaction of Siglec-like adhesins with different subsets of plasma glycoproteins could have a significant impact on the propensity of streptococci to establish endocardial infections.
29796594	10	68	theme	plasma	1543:1548	arg1	ligands					1550:1556	The four plasma ligands	1534:1556	The four plasma ligands	1534:1556	The four plasma ligands have potential roles in thrombosis and inflammation, and each has been cited as a biomarker for one or more vascular or other diseases.
29796594	0	69	theme	infective	111:119	arg1	endocarditis					121:132	infective endocarditis	111:132	infective endocarditis	111:132	Streptococcal Siglec-like adhesins recognize different subsets of human plasma glycoproteins: implications for infective endocarditis.
29796594	2	70	theme	binding	372:378	arg1	SLBRs					389:393	SLBRs	389:393	SLBRs	389:393	These organisms express cell surface serine-rich repeat adhesins containing "Siglec-like" binding regions (SLBRs) that mediate attachment to α2-3-linked sialic acids on human glycoproteins.
29796594	2	70	theme	binding	372:378	arg1	regions					380:386	"Siglec-like" binding regions	358:386	"Siglec-like" binding regions (SLBRs) that mediate attachment to α2-3-linked sialic acids on human glycoproteins	358:469	These organisms express cell surface serine-rich repeat adhesins containing "Siglec-like" binding regions (SLBRs) that mediate attachment to α2-3-linked sialic acids on human glycoproteins.
29796594	9	71	theme	C1	1404:1405	arg1	inhibitor					1407:1415	C1 inhibitor	1404:1415	C1 inhibitor	1404:1415	All four SLBRs recognize C1 inhibitor but detect distinctly different glycoforms of this key regulator of the complement and kallikrein protease cascades.
29796594	2	72	theme	sialic	435:440	arg1	acids					442:446	α2-3-linked sialic acids	423:446	α2-3-linked sialic acids	423:446	These organisms express cell surface serine-rich repeat adhesins containing "Siglec-like" binding regions (SLBRs) that mediate attachment to α2-3-linked sialic acids on human glycoproteins.
29796594	0	73	theme	Siglec-like	14:24	arg1	adhesins					26:33	Streptococcal Siglec-like adhesins	0:33	Streptococcal Siglec-like adhesins	0:33	Streptococcal Siglec-like adhesins recognize different subsets of human plasma glycoproteins: implications for infective endocarditis.
29796594	5	74	from	glycoproteins	863:875	arg1	plasma					886:891	human plasma	880:891	human plasma recognized by four SLBRs that prefer different α2-3 sialoglycan structures	880:966	In this report, we characterize the glycoproteins in human plasma recognized by four SLBRs that prefer different α2-3 sialoglycan structures.
29796594	3	75	theme	known	476:480	arg1	receptors					482:490	Two known receptors	472:490	Two known receptors for the Siglec-like adhesins	472:519	Two known receptors for the Siglec-like adhesins are the salivary mucin MG2/MUC7 and platelet GPIbα, and the interaction of streptococci with these targets may contribute to oral colonization and endocarditis, respectively.
29796594	3	75	theme	known	476:480	arg1	MG2/MUC7					544:551	the salivary mucin MG2/MUC7	525:551	the salivary mucin MG2/MUC7	525:551	Two known receptors for the Siglec-like adhesins are the salivary mucin MG2/MUC7 and platelet GPIbα, and the interaction of streptococci with these targets may contribute to oral colonization and endocarditis, respectively.
29796594	2	76	link	α2-3-linked	423:433	arg1	acids					442:446	α2-3-linked sialic acids	423:446	α2-3-linked sialic acids	423:446	These organisms express cell surface serine-rich repeat adhesins containing "Siglec-like" binding regions (SLBRs) that mediate attachment to α2-3-linked sialic acids on human glycoproteins.
29796594	7	77	theme	chain	1232:1236	arg1	H4					1238:1239	inter-alpha-trypsin inhibitor heavy chain H4	1196:1239	inter-alpha-trypsin inhibitor heavy chain H4	1196:1239	The preferred plasma protein ligands for a sialyl-T antigen-selective SLBR are proteoglycan 4 (lubricin) and inter-alpha-trypsin inhibitor heavy chain H4.
29796594	3	78	with	interaction	581:591	arg1	targets					620:626	these targets	614:626	these targets	614:626	Two known receptors for the Siglec-like adhesins are the salivary mucin MG2/MUC7 and platelet GPIbα, and the interaction of streptococci with these targets may contribute to oral colonization and endocarditis, respectively.
29796594	11	79	theme	different	1773:1781	arg1	subsets					1783:1789	different subsets	1773:1789	different subsets of plasma glycoproteins	1773:1813	The combined results suggest that the interaction of Siglec-like adhesins with different subsets of plasma glycoproteins could have a significant impact on the propensity of streptococci to establish endocardial infections.
29796594	3	80	theme	Siglec-like	500:510	arg1	adhesins					512:519	the Siglec-like adhesins	496:519	the Siglec-like adhesins	496:519	Two known receptors for the Siglec-like adhesins are the salivary mucin MG2/MUC7 and platelet GPIbα, and the interaction of streptococci with these targets may contribute to oral colonization and endocarditis, respectively.
29796594	7	81	theme	inhibitor	1216:1224	arg1	chain					1232:1236	inter-alpha-trypsin inhibitor heavy chain	1196:1236	inter-alpha-trypsin inhibitor heavy chain H4	1196:1239	The preferred plasma protein ligands for a sialyl-T antigen-selective SLBR are proteoglycan 4 (lubricin) and inter-alpha-trypsin inhibitor heavy chain H4.
29796594	0	82	dep	subsets	55:61	arg1	implications					94:105	implications	94:105	different subsets of human plasma glycoproteins: implications for infective endocarditis	45:132	Streptococcal Siglec-like adhesins recognize different subsets of human plasma glycoproteins: implications for infective endocarditis.
29796594	9	83	theme	regulator	1472:1480	arg1	glycoforms					1449:1458	distinctly different glycoforms	1428:1458	distinctly different glycoforms of this key regulator of the complement and kallikrein protease cascades	1428:1531	All four SLBRs recognize C1 inhibitor but detect distinctly different glycoforms of this key regulator of the complement and kallikrein protease cascades.
29796594	11	84	theme	glycoproteins	1801:1813	arg1	subsets					1783:1789	different subsets	1773:1789	different subsets of plasma glycoproteins	1773:1813	The combined results suggest that the interaction of Siglec-like adhesins with different subsets of plasma glycoproteins could have a significant impact on the propensity of streptococci to establish endocardial infections.
29796594	11	85	theme	combined	1698:1705	arg1	results					1707:1713	The combined results	1694:1713	The combined results	1694:1713	The combined results suggest that the interaction of Siglec-like adhesins with different subsets of plasma glycoproteins could have a significant impact on the propensity of streptococci to establish endocardial infections.
29796594	3	86	theme	salivary	529:536	arg1	receptors					482:490	Two known receptors	472:490	Two known receptors for the Siglec-like adhesins	472:519	Two known receptors for the Siglec-like adhesins are the salivary mucin MG2/MUC7 and platelet GPIbα, and the interaction of streptococci with these targets may contribute to oral colonization and endocarditis, respectively.
29796594	3	86	theme	salivary	529:536	arg1	MG2/MUC7					544:551	the salivary mucin MG2/MUC7	525:551	the salivary mucin MG2/MUC7	525:551	Two known receptors for the Siglec-like adhesins are the salivary mucin MG2/MUC7 and platelet GPIbα, and the interaction of streptococci with these targets may contribute to oral colonization and endocarditis, respectively.
29796594	1	87	theme	infective	258:266	arg1	endocarditis					268:279	infective endocarditis	258:279	infective endocarditis	258:279	Streptococcus gordonii and Streptococcus sanguinis are typically found among the normal oral microbiota but can also cause infective endocarditis.
29796594	6	88	gly	O-glycosylated	1071:1084	arg1	proteins					1041:1048	plasma proteins	1034:1048	plasma proteins that are extensively O-glycosylated	1034:1084	We found that the SLBRs recognize a surprisingly small subset of plasma proteins that are extensively O-glycosylated.
30860882	3	0	theme	Female	323:328	arg1	mice					337:340	Female C57Bl6 mice	323:340	Female C57Bl6 mice	323:340	Female C57Bl6 mice were fed chow or an obesogenic high-fat/high-sucrose (HF/HS) diet with subsequent mating of F1 and F2 female offspring to lean males to develop F2 and F3 generations, respectively.
30860882	7	1	theme	HF/HS	1121:1125	arg1	feeding					1127:1133	HF/HS feeding	1121:1133	HF/HS feeding	1121:1133	HF/HS feeding leads to increased periportal fibrosis and inflammation in chow-fed offspring without increased hepatic steatosis.
30860882	4	2	theme	acid	658:661	arg1	homeostasis					668:678	bile acid (BA) homeostasis	653:678	bile acid (BA) homeostasis	653:678	Offspring were fed chow or fibrogenic (high transfat, cholesterol, fructose) diets, and histopathological, metabolic changes, and bile acid (BA) homeostasis was evaluated.
30860882	5	3	theme	Chow-fed	695:702	arg1	offspring					707:715	Chow-fed F1 offspring	695:715	Chow-fed F1 offspring from maternal HF/HS lineages (HF/HS)	695:752	Chow-fed F1 offspring from maternal HF/HS lineages (HF/HS) developed periportal fibrosis and inflammation with aging, without differences in hepatic steatosis but increased BA pool size and shifts in BA composition.
30860882	3	4	theme	C57Bl6	330:335	arg1	mice					337:340	Female C57Bl6 mice	323:340	Female C57Bl6 mice	323:340	Female C57Bl6 mice were fed chow or an obesogenic high-fat/high-sucrose (HF/HS) diet with subsequent mating of F1 and F2 female offspring to lean males to develop F2 and F3 generations, respectively.
30860882	0	5	from	impact	18:23	arg1	homeostasis					76:86	offspring bile acid homeostasis	56:86	offspring bile acid homeostasis	56:86	Transgenerational impact of maternal obesogenic diet on offspring bile acid homeostasis and nonalcoholic fatty liver disease.
30860882	0	5	from	impact	18:23	arg1	disease					117:123	nonalcoholic fatty liver disease	92:123	nonalcoholic fatty liver disease	92:123	Transgenerational impact of maternal obesogenic diet on offspring bile acid homeostasis and nonalcoholic fatty liver disease.
30860882	1	6	theme	risk	161:164	arg1	factor					166:171	a risk factor	159:171	a risk factor for metabolic syndrome and nonalcoholic fatty liver disease (NAFLD) in offspring	159:252	Studies show maternal obesity is a risk factor for metabolic syndrome and nonalcoholic fatty liver disease (NAFLD) in offspring.
30860882	4	7	theme	fibrogenic	550:559	arg1	diets					600:604	fibrogenic (high transfat, cholesterol, fructose) diets	550:604	fibrogenic (high transfat, cholesterol, fructose) diets	550:604	Offspring were fed chow or fibrogenic (high transfat, cholesterol, fructose) diets, and histopathological, metabolic changes, and bile acid (BA) homeostasis was evaluated.
30860882	0	8	theme	fatty	105:109	arg1	disease					117:123	nonalcoholic fatty liver disease	92:123	nonalcoholic fatty liver disease	92:123	Transgenerational impact of maternal obesogenic diet on offspring bile acid homeostasis and nonalcoholic fatty liver disease.
30860882	5	9	from	lineages	737:744	arg1	offspring					707:715	Chow-fed F1 offspring	695:715	Chow-fed F1 offspring from maternal HF/HS lineages (HF/HS)	695:752	Chow-fed F1 offspring from maternal HF/HS lineages (HF/HS) developed periportal fibrosis and inflammation with aging, without differences in hepatic steatosis but increased BA pool size and shifts in BA composition.
30860882	7	10	from	fibrosis	1165:1172	arg1	offspring					1203:1211	chow-fed offspring	1194:1211	chow-fed offspring without increased hepatic steatosis	1194:1247	HF/HS feeding leads to increased periportal fibrosis and inflammation in chow-fed offspring without increased hepatic steatosis.
30860882	1	11	dep	show	134:137	arg1	factor					166:171	a risk factor	159:171	a risk factor for metabolic syndrome and nonalcoholic fatty liver disease (NAFLD) in offspring	159:252	Studies show maternal obesity is a risk factor for metabolic syndrome and nonalcoholic fatty liver disease (NAFLD) in offspring.
30860882	6	12	theme	F1	1065:1066	arg1	offspring					1068:1076	F1 offspring	1065:1076	F1 offspring	1065:1076	F1, but not F2 or F3, offspring from HF/HS showed increased steatosis on a fibrogenic diet, yet inflammation and fibrosis were paradoxically decreased in F1 offspring, a trend continued in F2 and F3 offspring.
30860882	0	13	theme	nonalcoholic	92:103	arg1	disease					117:123	nonalcoholic fatty liver disease	92:123	nonalcoholic fatty liver disease	92:123	Transgenerational impact of maternal obesogenic diet on offspring bile acid homeostasis and nonalcoholic fatty liver disease.
30860882	3	14	with	diet	403:406	arg1	mating					424:429	subsequent mating	413:429	subsequent mating of F1 and F2 female offspring to lean males	413:473	Female C57Bl6 mice were fed chow or an obesogenic high-fat/high-sucrose (HF/HS) diet with subsequent mating of F1 and F2 female offspring to lean males to develop F2 and F3 generations, respectively.
30860882	7	15	theme	periportal	1154:1163	arg1	fibrosis					1165:1172	increased periportal fibrosis	1144:1172	increased periportal fibrosis	1144:1172	HF/HS feeding leads to increased periportal fibrosis and inflammation in chow-fed offspring without increased hepatic steatosis.
30860882	2	16	theme	potential	273:281	arg1	mechanisms					283:292	potential mechanisms	273:292	potential mechanisms underlying these phenotypes	273:320	Here we evaluated potential mechanisms underlying these phenotypes.
30860882	9	17	theme	complex	1412:1418	arg1	adaptations					1420:1430	complex adaptations	1412:1430	complex adaptations in NAFLD phenotypes with maternal diet	1412:1469	These findings highlight complex adaptations in NAFLD phenotypes with maternal diet.
30860882	4	18	theme	high	562:565	arg1	cholesterol					577:587	cholesterol	577:587	cholesterol	577:587	Offspring were fed chow or fibrogenic (high transfat, cholesterol, fructose) diets, and histopathological, metabolic changes, and bile acid (BA) homeostasis was evaluated.
30860882	4	18	theme	high	562:565	arg1	transfat					567:574	high transfat	562:574	high transfat	562:574	Offspring were fed chow or fibrogenic (high transfat, cholesterol, fructose) diets, and histopathological, metabolic changes, and bile acid (BA) homeostasis was evaluated.
30860882	4	18	theme	high	562:565	arg1	fructose					590:597	fructose	590:597	fructose	590:597	Offspring were fed chow or fibrogenic (high transfat, cholesterol, fructose) diets, and histopathological, metabolic changes, and bile acid (BA) homeostasis was evaluated.
30860882	3	19	theme	fed	347:349	arg1	chow					351:354	fed chow	347:354	fed chow	347:354	Female C57Bl6 mice were fed chow or an obesogenic high-fat/high-sucrose (HF/HS) diet with subsequent mating of F1 and F2 female offspring to lean males to develop F2 and F3 generations, respectively.
30860882	7	20	theme	chow-fed	1194:1201	arg1	offspring					1203:1211	chow-fed offspring	1194:1211	chow-fed offspring without increased hepatic steatosis	1194:1247	HF/HS feeding leads to increased periportal fibrosis and inflammation in chow-fed offspring without increased hepatic steatosis.
30860882	8	21	from	dams	1307:1310	arg1	offspring					1286:1294	fibrogenic diet-fed F1 offspring	1263:1294	fibrogenic diet-fed F1 offspring from HF/HS dams	1263:1310	By contrast, fibrogenic diet-fed F1 offspring from HF/HS dams exhibited worse hepatic steatosis but decreased inflammation and fibrosis.
30860882	6	22	dep	F2	1100:1101	arg1	offspring					1110:1118	offspring	1110:1118	offspring	1110:1118	F1, but not F2 or F3, offspring from HF/HS showed increased steatosis on a fibrogenic diet, yet inflammation and fibrosis were paradoxically decreased in F1 offspring, a trend continued in F2 and F3 offspring.
30860882	6	23	theme	fibrogenic	986:995	arg1	diet					997:1000	a fibrogenic diet	984:1000	a fibrogenic diet	984:1000	F1, but not F2 or F3, offspring from HF/HS showed increased steatosis on a fibrogenic diet, yet inflammation and fibrosis were paradoxically decreased in F1 offspring, a trend continued in F2 and F3 offspring.
30860882	3	24	theme	F1	434:435	arg1	offspring					451:459	F1 and F2 female offspring	434:459	F1 and F2 female offspring	434:459	Female C57Bl6 mice were fed chow or an obesogenic high-fat/high-sucrose (HF/HS) diet with subsequent mating of F1 and F2 female offspring to lean males to develop F2 and F3 generations, respectively.
30860882	0	25	theme	liver	111:115	arg1	disease					117:123	nonalcoholic fatty liver disease	92:123	nonalcoholic fatty liver disease	92:123	Transgenerational impact of maternal obesogenic diet on offspring bile acid homeostasis and nonalcoholic fatty liver disease.
30860882	5	26	theme	HF/HS	731:735	arg1	HF/HS					747:751	HF/HS	747:751	HF/HS	747:751	Chow-fed F1 offspring from maternal HF/HS lineages (HF/HS) developed periportal fibrosis and inflammation with aging, without differences in hepatic steatosis but increased BA pool size and shifts in BA composition.
30860882	5	26	theme	HF/HS	731:735	arg1	lineages					737:744	maternal HF/HS lineages	722:744	maternal HF/HS lineages (HF/HS)	722:752	Chow-fed F1 offspring from maternal HF/HS lineages (HF/HS) developed periportal fibrosis and inflammation with aging, without differences in hepatic steatosis but increased BA pool size and shifts in BA composition.
30860882	7	27	from	inflammation	1178:1189	arg1	offspring					1203:1211	chow-fed offspring	1194:1211	chow-fed offspring without increased hepatic steatosis	1194:1247	HF/HS feeding leads to increased periportal fibrosis and inflammation in chow-fed offspring without increased hepatic steatosis.
30860882	0	28	theme	Transgenerational	0:16	arg1	impact					18:23	Transgenerational impact	0:23	Transgenerational impact of maternal obesogenic diet on offspring bile acid homeostasis and nonalcoholic fatty liver disease.	0:124	Transgenerational impact of maternal obesogenic diet on offspring bile acid homeostasis and nonalcoholic fatty liver disease.
30860882	4	29	theme	histopathological	611:627	arg1	changes					640:646	metabolic changes	630:646	metabolic changes	630:646	Offspring were fed chow or fibrogenic (high transfat, cholesterol, fructose) diets, and histopathological, metabolic changes, and bile acid (BA) homeostasis was evaluated.
30860882	5	30	theme	maternal	722:729	arg1	HF/HS					747:751	HF/HS	747:751	HF/HS	747:751	Chow-fed F1 offspring from maternal HF/HS lineages (HF/HS) developed periportal fibrosis and inflammation with aging, without differences in hepatic steatosis but increased BA pool size and shifts in BA composition.
30860882	5	30	theme	maternal	722:729	arg1	lineages					737:744	maternal HF/HS lineages	722:744	maternal HF/HS lineages (HF/HS)	722:752	Chow-fed F1 offspring from maternal HF/HS lineages (HF/HS) developed periportal fibrosis and inflammation with aging, without differences in hepatic steatosis but increased BA pool size and shifts in BA composition.
30860882	9	31	with	phenotypes	1441:1450	arg1	diet					1466:1469	maternal diet	1457:1469	maternal diet	1457:1469	These findings highlight complex adaptations in NAFLD phenotypes with maternal diet.
30860882	5	32	theme	F1	704:705	arg1	offspring					707:715	Chow-fed F1 offspring	695:715	Chow-fed F1 offspring from maternal HF/HS lineages (HF/HS)	695:752	Chow-fed F1 offspring from maternal HF/HS lineages (HF/HS) developed periportal fibrosis and inflammation with aging, without differences in hepatic steatosis but increased BA pool size and shifts in BA composition.
30860882	3	33	dep	F2	486:487	arg1	generations					496:506	generations	496:506	generations	496:506	Female C57Bl6 mice were fed chow or an obesogenic high-fat/high-sucrose (HF/HS) diet with subsequent mating of F1 and F2 female offspring to lean males to develop F2 and F3 generations, respectively.
30860882	1	34	theme	metabolic	177:185	arg1	syndrome					187:194	metabolic syndrome	177:194	metabolic syndrome	177:194	Studies show maternal obesity is a risk factor for metabolic syndrome and nonalcoholic fatty liver disease (NAFLD) in offspring.
30860882	3	35	theme	HF/HS	396:400	arg1	diet					403:406	an obesogenic high-fat/high-sucrose (HF/HS) diet	359:406	an obesogenic high-fat/high-sucrose (HF/HS) diet with subsequent mating of F1 and F2 female offspring to lean males to develop F2 and F3 generations	359:506	Female C57Bl6 mice were fed chow or an obesogenic high-fat/high-sucrose (HF/HS) diet with subsequent mating of F1 and F2 female offspring to lean males to develop F2 and F3 generations, respectively.
30860882	4	36	theme	metabolic	630:638	arg1	changes					640:646	metabolic changes	630:646	metabolic changes	630:646	Offspring were fed chow or fibrogenic (high transfat, cholesterol, fructose) diets, and histopathological, metabolic changes, and bile acid (BA) homeostasis was evaluated.
30860882	0	37	theme	maternal	28:35	arg1	diet					48:51	maternal obesogenic diet	28:51	maternal obesogenic diet	28:51	Transgenerational impact of maternal obesogenic diet on offspring bile acid homeostasis and nonalcoholic fatty liver disease.
30860882	5	38	theme	BA	895:896	arg1	composition					898:908	BA composition	895:908	BA composition	895:908	Chow-fed F1 offspring from maternal HF/HS lineages (HF/HS) developed periportal fibrosis and inflammation with aging, without differences in hepatic steatosis but increased BA pool size and shifts in BA composition.
30860882	3	39	theme	offspring	451:459	arg1	mating					424:429	subsequent mating	413:429	subsequent mating of F1 and F2 female offspring to lean males	413:473	Female C57Bl6 mice were fed chow or an obesogenic high-fat/high-sucrose (HF/HS) diet with subsequent mating of F1 and F2 female offspring to lean males to develop F2 and F3 generations, respectively.
30860882	3	40	theme	high-fat/high-sucrose	373:393	arg1	diet					403:406	an obesogenic high-fat/high-sucrose (HF/HS) diet	359:406	an obesogenic high-fat/high-sucrose (HF/HS) diet with subsequent mating of F1 and F2 female offspring to lean males to develop F2 and F3 generations	359:506	Female C57Bl6 mice were fed chow or an obesogenic high-fat/high-sucrose (HF/HS) diet with subsequent mating of F1 and F2 female offspring to lean males to develop F2 and F3 generations, respectively.
30860882	8	41	theme	worse	1322:1326	arg1	steatosis					1336:1344	worse hepatic steatosis	1322:1344	worse hepatic steatosis	1322:1344	By contrast, fibrogenic diet-fed F1 offspring from HF/HS dams exhibited worse hepatic steatosis but decreased inflammation and fibrosis.
30860882	4	42	theme	bile	653:656	arg1	BA					664:665	BA	664:665	BA	664:665	Offspring were fed chow or fibrogenic (high transfat, cholesterol, fructose) diets, and histopathological, metabolic changes, and bile acid (BA) homeostasis was evaluated.
30860882	4	42	theme	bile	653:656	arg1	acid					658:661	bile acid	653:661	bile acid (BA) homeostasis	653:678	Offspring were fed chow or fibrogenic (high transfat, cholesterol, fructose) diets, and histopathological, metabolic changes, and bile acid (BA) homeostasis was evaluated.
30860882	1	43	theme	nonalcoholic	200:211	arg1	NAFLD					234:238	NAFLD	234:238	NAFLD	234:238	Studies show maternal obesity is a risk factor for metabolic syndrome and nonalcoholic fatty liver disease (NAFLD) in offspring.
30860882	1	43	theme	nonalcoholic	200:211	arg1	disease					225:231	nonalcoholic fatty liver disease	200:231	nonalcoholic fatty liver disease (NAFLD)	200:239	Studies show maternal obesity is a risk factor for metabolic syndrome and nonalcoholic fatty liver disease (NAFLD) in offspring.
30860882	0	44	theme	diet	48:51	arg1	impact					18:23	Transgenerational impact	0:23	Transgenerational impact of maternal obesogenic diet on offspring bile acid homeostasis and nonalcoholic fatty liver disease.	0:124	Transgenerational impact of maternal obesogenic diet on offspring bile acid homeostasis and nonalcoholic fatty liver disease.
30860882	7	45	theme	increased	1221:1229	arg1	steatosis					1239:1247	increased hepatic steatosis	1221:1247	increased hepatic steatosis	1221:1247	HF/HS feeding leads to increased periportal fibrosis and inflammation in chow-fed offspring without increased hepatic steatosis.
30860882	3	46	theme	F2	441:442	arg1	offspring					451:459	F1 and F2 female offspring	434:459	F1 and F2 female offspring	434:459	Female C57Bl6 mice were fed chow or an obesogenic high-fat/high-sucrose (HF/HS) diet with subsequent mating of F1 and F2 female offspring to lean males to develop F2 and F3 generations, respectively.
30860882	8	47	theme	hepatic	1328:1334	arg1	steatosis					1336:1344	worse hepatic steatosis	1322:1344	worse hepatic steatosis	1322:1344	By contrast, fibrogenic diet-fed F1 offspring from HF/HS dams exhibited worse hepatic steatosis but decreased inflammation and fibrosis.
30860882	1	48	theme	fatty	213:217	arg1	NAFLD					234:238	NAFLD	234:238	NAFLD	234:238	Studies show maternal obesity is a risk factor for metabolic syndrome and nonalcoholic fatty liver disease (NAFLD) in offspring.
30860882	1	48	theme	fatty	213:217	arg1	disease					225:231	nonalcoholic fatty liver disease	200:231	nonalcoholic fatty liver disease (NAFLD)	200:239	Studies show maternal obesity is a risk factor for metabolic syndrome and nonalcoholic fatty liver disease (NAFLD) in offspring.
30860882	0	49	theme	obesogenic	37:46	arg1	diet					48:51	maternal obesogenic diet	28:51	maternal obesogenic diet	28:51	Transgenerational impact of maternal obesogenic diet on offspring bile acid homeostasis and nonalcoholic fatty liver disease.
30860882	3	50	theme	subsequent	413:422	arg1	mating					424:429	subsequent mating	413:429	subsequent mating of F1 and F2 female offspring to lean males	413:473	Female C57Bl6 mice were fed chow or an obesogenic high-fat/high-sucrose (HF/HS) diet with subsequent mating of F1 and F2 female offspring to lean males to develop F2 and F3 generations, respectively.
30860882	9	51	theme	NAFLD	1435:1439	arg1	phenotypes					1441:1450	NAFLD phenotypes	1435:1450	NAFLD phenotypes with maternal diet	1435:1469	These findings highlight complex adaptations in NAFLD phenotypes with maternal diet.
30860882	3	52	theme	obesogenic	362:371	arg1	diet					403:406	an obesogenic high-fat/high-sucrose (HF/HS) diet	359:406	an obesogenic high-fat/high-sucrose (HF/HS) diet with subsequent mating of F1 and F2 female offspring to lean males to develop F2 and F3 generations	359:506	Female C57Bl6 mice were fed chow or an obesogenic high-fat/high-sucrose (HF/HS) diet with subsequent mating of F1 and F2 female offspring to lean males to develop F2 and F3 generations, respectively.
30860882	1	53	theme	liver	219:223	arg1	NAFLD					234:238	NAFLD	234:238	NAFLD	234:238	Studies show maternal obesity is a risk factor for metabolic syndrome and nonalcoholic fatty liver disease (NAFLD) in offspring.
30860882	1	53	theme	liver	219:223	arg1	disease					225:231	nonalcoholic fatty liver disease	200:231	nonalcoholic fatty liver disease (NAFLD)	200:239	Studies show maternal obesity is a risk factor for metabolic syndrome and nonalcoholic fatty liver disease (NAFLD) in offspring.
30860882	0	54	theme	offspring	56:64	arg1	homeostasis					76:86	offspring bile acid homeostasis	56:86	offspring bile acid homeostasis	56:86	Transgenerational impact of maternal obesogenic diet on offspring bile acid homeostasis and nonalcoholic fatty liver disease.
30860882	8	55	theme	HF/HS	1301:1305	arg1	dams					1307:1310	HF/HS dams	1301:1310	HF/HS dams	1301:1310	By contrast, fibrogenic diet-fed F1 offspring from HF/HS dams exhibited worse hepatic steatosis but decreased inflammation and fibrosis.
30860882	4	56	dep	diets	600:604	arg1	cholesterol					577:587	cholesterol	577:587	cholesterol	577:587	Offspring were fed chow or fibrogenic (high transfat, cholesterol, fructose) diets, and histopathological, metabolic changes, and bile acid (BA) homeostasis was evaluated.
30860882	4	56	dep	diets	600:604	arg1	transfat					567:574	high transfat	562:574	high transfat	562:574	Offspring were fed chow or fibrogenic (high transfat, cholesterol, fructose) diets, and histopathological, metabolic changes, and bile acid (BA) homeostasis was evaluated.
30860882	4	56	dep	diets	600:604	arg1	fructose					590:597	fructose	590:597	fructose	590:597	Offspring were fed chow or fibrogenic (high transfat, cholesterol, fructose) diets, and histopathological, metabolic changes, and bile acid (BA) homeostasis was evaluated.
30860882	5	57	theme	BA	868:869	arg1	size					876:879	BA pool size	868:879	BA pool size	868:879	Chow-fed F1 offspring from maternal HF/HS lineages (HF/HS) developed periportal fibrosis and inflammation with aging, without differences in hepatic steatosis but increased BA pool size and shifts in BA composition.
30860882	5	58	theme	periportal	764:773	arg1	fibrosis					775:782	periportal fibrosis	764:782	periportal fibrosis	764:782	Chow-fed F1 offspring from maternal HF/HS lineages (HF/HS) developed periportal fibrosis and inflammation with aging, without differences in hepatic steatosis but increased BA pool size and shifts in BA composition.
30860882	5	59	theme	pool	871:874	arg1	size					876:879	BA pool size	868:879	BA pool size	868:879	Chow-fed F1 offspring from maternal HF/HS lineages (HF/HS) developed periportal fibrosis and inflammation with aging, without differences in hepatic steatosis but increased BA pool size and shifts in BA composition.
30860882	3	60	with	chow	351:354	arg1	mating					424:429	subsequent mating	413:429	subsequent mating of F1 and F2 female offspring to lean males	413:473	Female C57Bl6 mice were fed chow or an obesogenic high-fat/high-sucrose (HF/HS) diet with subsequent mating of F1 and F2 female offspring to lean males to develop F2 and F3 generations, respectively.
30860882	3	61	theme	lean	464:467	arg1	males					469:473	lean males	464:473	lean males	464:473	Female C57Bl6 mice were fed chow or an obesogenic high-fat/high-sucrose (HF/HS) diet with subsequent mating of F1 and F2 female offspring to lean males to develop F2 and F3 generations, respectively.
30860882	8	62	theme	F1	1283:1284	arg1	offspring					1286:1294	fibrogenic diet-fed F1 offspring	1263:1294	fibrogenic diet-fed F1 offspring from HF/HS dams	1263:1310	By contrast, fibrogenic diet-fed F1 offspring from HF/HS dams exhibited worse hepatic steatosis but decreased inflammation and fibrosis.
30860882	8	63	theme	diet-fed	1274:1281	arg1	offspring					1286:1294	fibrogenic diet-fed F1 offspring	1263:1294	fibrogenic diet-fed F1 offspring from HF/HS dams	1263:1310	By contrast, fibrogenic diet-fed F1 offspring from HF/HS dams exhibited worse hepatic steatosis but decreased inflammation and fibrosis.
30860882	5	64	from	differences	821:831	arg1	steatosis					844:852	hepatic steatosis	836:852	hepatic steatosis	836:852	Chow-fed F1 offspring from maternal HF/HS lineages (HF/HS) developed periportal fibrosis and inflammation with aging, without differences in hepatic steatosis but increased BA pool size and shifts in BA composition.
30860882	1	65	theme	maternal	139:146	arg1	obesity					148:154	maternal obesity	139:154	maternal obesity	139:154	Studies show maternal obesity is a risk factor for metabolic syndrome and nonalcoholic fatty liver disease (NAFLD) in offspring.
30860882	8	66	theme	fibrogenic	1263:1272	arg1	offspring					1286:1294	fibrogenic diet-fed F1 offspring	1263:1294	fibrogenic diet-fed F1 offspring from HF/HS dams	1263:1310	By contrast, fibrogenic diet-fed F1 offspring from HF/HS dams exhibited worse hepatic steatosis but decreased inflammation and fibrosis.
30860882	9	67	theme	maternal	1457:1464	arg1	diet					1466:1469	maternal diet	1457:1469	maternal diet	1457:1469	These findings highlight complex adaptations in NAFLD phenotypes with maternal diet.
30860882	0	68	theme	acid	71:74	arg1	homeostasis					76:86	offspring bile acid homeostasis	56:86	offspring bile acid homeostasis	56:86	Transgenerational impact of maternal obesogenic diet on offspring bile acid homeostasis and nonalcoholic fatty liver disease.
30860882	3	69	theme	female	444:449	arg1	offspring					451:459	F1 and F2 female offspring	434:459	F1 and F2 female offspring	434:459	Female C57Bl6 mice were fed chow or an obesogenic high-fat/high-sucrose (HF/HS) diet with subsequent mating of F1 and F2 female offspring to lean males to develop F2 and F3 generations, respectively.
30860882	7	70	theme	hepatic	1231:1237	arg1	steatosis					1239:1247	increased hepatic steatosis	1221:1247	increased hepatic steatosis	1221:1247	HF/HS feeding leads to increased periportal fibrosis and inflammation in chow-fed offspring without increased hepatic steatosis.
30860882	5	71	theme	hepatic	836:842	arg1	steatosis					844:852	hepatic steatosis	836:852	hepatic steatosis	836:852	Chow-fed F1 offspring from maternal HF/HS lineages (HF/HS) developed periportal fibrosis and inflammation with aging, without differences in hepatic steatosis but increased BA pool size and shifts in BA composition.
30860882	9	72	from	adaptations	1420:1430	arg1	phenotypes					1441:1450	NAFLD phenotypes	1435:1450	NAFLD phenotypes with maternal diet	1435:1469	These findings highlight complex adaptations in NAFLD phenotypes with maternal diet.
30860882	0	73	theme	bile	66:69	arg1	homeostasis					76:86	offspring bile acid homeostasis	56:86	offspring bile acid homeostasis	56:86	Transgenerational impact of maternal obesogenic diet on offspring bile acid homeostasis and nonalcoholic fatty liver disease.
30860882	6	74	dep	decreased	1052:1060	arg1	continued					1087:1095	continued	1087:1095	continued in F2 and F3 offspring	1087:1118	F1, but not F2 or F3, offspring from HF/HS showed increased steatosis on a fibrogenic diet, yet inflammation and fibrosis were paradoxically decreased in F1 offspring, a trend continued in F2 and F3 offspring.
30860882	1	75	from	factor	166:171	arg1	offspring					244:252	offspring	244:252	offspring	244:252	Studies show maternal obesity is a risk factor for metabolic syndrome and nonalcoholic fatty liver disease (NAFLD) in offspring.
30860882	7	76	theme	increased	1144:1152	arg1	fibrosis					1165:1172	increased periportal fibrosis	1144:1172	increased periportal fibrosis	1144:1172	HF/HS feeding leads to increased periportal fibrosis and inflammation in chow-fed offspring without increased hepatic steatosis.
30860882	6	77	theme	increased	961:969	arg1	steatosis					971:979	increased steatosis	961:979	increased steatosis	961:979	F1, but not F2 or F3, offspring from HF/HS showed increased steatosis on a fibrogenic diet, yet inflammation and fibrosis were paradoxically decreased in F1 offspring, a trend continued in F2 and F3 offspring.
30562060	4	0	from	changes	897:903	arg1	transcriptome					951:963	enteroendocrine cell transcriptome	930:963	enteroendocrine cell transcriptome in the ileum and colon	930:986	We fed GPR41-red fluorescent protein mice, an enteroendocrine reporter mouse strain, chow, high-fat diet (HFD), or HFD supplemented either with 10% nonfermentable fiber cellulose or fermentable flaxseed fibers for 12 wk to assess changes in cecal gut microbiota, enteroendocrine cell transcriptome in the ileum and colon, and physiological parameters.
30562060	4	0	from	changes	897:903	arg1	parameters					1007:1016	physiological parameters	993:1016	physiological parameters	993:1016	We fed GPR41-red fluorescent protein mice, an enteroendocrine reporter mouse strain, chow, high-fat diet (HFD), or HFD supplemented either with 10% nonfermentable fiber cellulose or fermentable flaxseed fibers for 12 wk to assess changes in cecal gut microbiota, enteroendocrine cell transcriptome in the ileum and colon, and physiological parameters.
30562060	4	0	from	changes	897:903	arg1	microbiota					918:927	cecal gut microbiota	908:927	cecal gut microbiota	908:927	We fed GPR41-red fluorescent protein mice, an enteroendocrine reporter mouse strain, chow, high-fat diet (HFD), or HFD supplemented either with 10% nonfermentable fiber cellulose or fermentable flaxseed fibers for 12 wk to assess changes in cecal gut microbiota, enteroendocrine cell transcriptome in the ileum and colon, and physiological parameters.
30562060	4	1	theme	fiber	830:834	arg1	cellulose					836:844	10% nonfermentable fiber cellulose	811:844	10% nonfermentable fiber cellulose	811:844	We fed GPR41-red fluorescent protein mice, an enteroendocrine reporter mouse strain, chow, high-fat diet (HFD), or HFD supplemented either with 10% nonfermentable fiber cellulose or fermentable flaxseed fibers for 12 wk to assess changes in cecal gut microbiota, enteroendocrine cell transcriptome in the ileum and colon, and physiological parameters.
30562060	4	2	theme	physiological	993:1005	arg1	parameters					1007:1016	physiological parameters	993:1016	physiological parameters	993:1016	We fed GPR41-red fluorescent protein mice, an enteroendocrine reporter mouse strain, chow, high-fat diet (HFD), or HFD supplemented either with 10% nonfermentable fiber cellulose or fermentable flaxseed fibers for 12 wk to assess changes in cecal gut microbiota, enteroendocrine cell transcriptome in the ileum and colon, and physiological parameters.
30562060	8	3	theme	flaxseed	1662:1669	arg1	fibers					1671:1676	flaxseed fibers	1662:1676	flaxseed fibers	1662:1676	Our study shows that flaxseed fibers alter cecal microbial ecology, are fermented to SCFAs in the cecum, and modulate enteroendocrine cell transcriptome in the colon, which may contribute to their metabolically favorable phenotype.
30562060	1	4	theme	microbiota	254:263	arg1	activity					234:241	the enzymatic activity	220:241	the enzymatic activity of the gut microbiota for complete metabolism into short-chain fatty acids (SCFAs)	220:324	Dietary fibers, an integral part of the human diet, require the enzymatic activity of the gut microbiota for complete metabolism into short-chain fatty acids (SCFAs).
30562060	6	5	theme	butyrate	1247:1254	arg1	levels					1227:1232	levels	1227:1232	levels of the SCFAs butyrate similar to the chow diet	1227:1279	The shifts in cecal bacterial composition restored levels of the SCFAs butyrate similar to the chow diet, resulting in colonic but not ileal enteroendocrine cell transcriptional changes in genes related to cell cycle, mRNA, and protein transport compared with HFD.
30562060	1	6	theme	complete	269:276	arg1	metabolism					278:287	complete metabolism	269:287	complete metabolism into short-chain fatty acids (SCFAs)	269:324	Dietary fibers, an integral part of the human diet, require the enzymatic activity of the gut microbiota for complete metabolism into short-chain fatty acids (SCFAs).
30562060	8	7	theme	microbial	1690:1698	arg1	ecology					1700:1706	cecal microbial ecology	1684:1706	cecal microbial ecology	1684:1706	Our study shows that flaxseed fibers alter cecal microbial ecology, are fermented to SCFAs in the cecum, and modulate enteroendocrine cell transcriptome in the colon, which may contribute to their metabolically favorable phenotype.
30562060	4	8	theme	%	813:813	arg1	cellulose					836:844	10% nonfermentable fiber cellulose	811:844	10% nonfermentable fiber cellulose	811:844	We fed GPR41-red fluorescent protein mice, an enteroendocrine reporter mouse strain, chow, high-fat diet (HFD), or HFD supplemented either with 10% nonfermentable fiber cellulose or fermentable flaxseed fibers for 12 wk to assess changes in cecal gut microbiota, enteroendocrine cell transcriptome in the ileum and colon, and physiological parameters.
30562060	1	9	theme	human	200:204	arg1	diet					206:209	the human diet	196:209	the human diet	196:209	Dietary fibers, an integral part of the human diet, require the enzymatic activity of the gut microbiota for complete metabolism into short-chain fatty acids (SCFAs).
30562060	6	10	dep	resulting	1282:1290	arg1	compared					1422:1429	compared	1422:1429	compared with HFD	1422:1438	The shifts in cecal bacterial composition restored levels of the SCFAs butyrate similar to the chow diet, resulting in colonic but not ileal enteroendocrine cell transcriptional changes in genes related to cell cycle, mRNA, and protein transport compared with HFD.
30562060	0	11	theme	diet-induced	138:149	arg1	obesity					151:157	diet-induced obesity	138:157	diet-induced obesity	138:157	Microbial fermentation of flaxseed fibers modulates the transcriptome of GPR41-expressing enteroendocrine cells and protects mice against diet-induced obesity.
30562060	5	12	theme	flaxseed	1036:1043	arg1	fibers					1045:1050	flaxseed fibers	1036:1050	flaxseed fibers	1036:1050	We observed that flaxseed fibers restructured the gut microbiota and promoted proliferation of the genera Bifidobacterium and Akkermansia compared with HFD.
30562060	4	13	theme	flaxseed	861:868	arg1	fibers					870:875	fermentable flaxseed fibers	849:875	fermentable flaxseed fibers	849:875	We fed GPR41-red fluorescent protein mice, an enteroendocrine reporter mouse strain, chow, high-fat diet (HFD), or HFD supplemented either with 10% nonfermentable fiber cellulose or fermentable flaxseed fibers for 12 wk to assess changes in cecal gut microbiota, enteroendocrine cell transcriptome in the ileum and colon, and physiological parameters.
30562060	6	14	theme	bacterial	1196:1204	arg1	composition					1206:1216	cecal bacterial composition	1190:1216	cecal bacterial composition	1190:1216	The shifts in cecal bacterial composition restored levels of the SCFAs butyrate similar to the chow diet, resulting in colonic but not ileal enteroendocrine cell transcriptional changes in genes related to cell cycle, mRNA, and protein transport compared with HFD.
30562060	3	15	theme	fermentation	563:574	arg1	profiles					576:583	their fermentation profiles	557:583	their fermentation profiles	557:583	Flaxseed fibers improve metabolism in rodents and mice, but their fermentation profiles, effects on enteroendocrine cells, and associated metabolic benefits are unknown.
30562060	6	16	theme	chow	1271:1274	arg1	diet					1276:1279	the chow diet	1267:1279	the chow diet	1267:1279	The shifts in cecal bacterial composition restored levels of the SCFAs butyrate similar to the chow diet, resulting in colonic but not ileal enteroendocrine cell transcriptional changes in genes related to cell cycle, mRNA, and protein transport compared with HFD.
30562060	1	17	theme	Dietary	160:166	arg1	part					188:191	an integral part	176:191	an integral part of the human diet	176:209	Dietary fibers, an integral part of the human diet, require the enzymatic activity of the gut microbiota for complete metabolism into short-chain fatty acids (SCFAs).
30562060	1	17	theme	Dietary	160:166	arg1	fibers					168:173	Dietary fibers	160:173	Dietary fibers	160:173	Dietary fibers, an integral part of the human diet, require the enzymatic activity of the gut microbiota for complete metabolism into short-chain fatty acids (SCFAs).
30562060	4	18	theme	cell	946:949	arg1	transcriptome					951:963	enteroendocrine cell transcriptome	930:963	enteroendocrine cell transcriptome in the ileum and colon	930:986	We fed GPR41-red fluorescent protein mice, an enteroendocrine reporter mouse strain, chow, high-fat diet (HFD), or HFD supplemented either with 10% nonfermentable fiber cellulose or fermentable flaxseed fibers for 12 wk to assess changes in cecal gut microbiota, enteroendocrine cell transcriptome in the ileum and colon, and physiological parameters.
30562060	4	19	theme	fluorescent	684:694	arg1	mice					704:707	GPR41-red fluorescent protein mice	674:707	GPR41-red fluorescent protein mice	674:707	We fed GPR41-red fluorescent protein mice, an enteroendocrine reporter mouse strain, chow, high-fat diet (HFD), or HFD supplemented either with 10% nonfermentable fiber cellulose or fermentable flaxseed fibers for 12 wk to assess changes in cecal gut microbiota, enteroendocrine cell transcriptome in the ileum and colon, and physiological parameters.
30562060	4	19	theme	fluorescent	684:694	arg1	strain					744:749	an enteroendocrine reporter mouse strain	710:749	an enteroendocrine reporter mouse strain	710:749	We fed GPR41-red fluorescent protein mice, an enteroendocrine reporter mouse strain, chow, high-fat diet (HFD), or HFD supplemented either with 10% nonfermentable fiber cellulose or fermentable flaxseed fibers for 12 wk to assess changes in cecal gut microbiota, enteroendocrine cell transcriptome in the ileum and colon, and physiological parameters.
30562060	3	20	from	metabolism	521:530	arg1	mice					547:550	mice	547:550	mice	547:550	Flaxseed fibers improve metabolism in rodents and mice, but their fermentation profiles, effects on enteroendocrine cells, and associated metabolic benefits are unknown.
30562060	3	20	from	metabolism	521:530	arg1	rodents					535:541	rodents	535:541	rodents	535:541	Flaxseed fibers improve metabolism in rodents and mice, but their fermentation profiles, effects on enteroendocrine cells, and associated metabolic benefits are unknown.
30562060	0	21	theme	enteroendocrine	90:104	arg1	cells					106:110	GPR41-expressing enteroendocrine cells	73:110	GPR41-expressing enteroendocrine cells	73:110	Microbial fermentation of flaxseed fibers modulates the transcriptome of GPR41-expressing enteroendocrine cells and protects mice against diet-induced obesity.
30562060	8	22	theme	cell	1775:1778	arg1	transcriptome					1780:1792	enteroendocrine cell transcriptome	1759:1792	enteroendocrine cell transcriptome	1759:1792	Our study shows that flaxseed fibers alter cecal microbial ecology, are fermented to SCFAs in the cecum, and modulate enteroendocrine cell transcriptome in the colon, which may contribute to their metabolically favorable phenotype.
30562060	1	23	theme	integral	179:186	arg1	part					188:191	an integral part	176:191	an integral part of the human diet	176:209	Dietary fibers, an integral part of the human diet, require the enzymatic activity of the gut microbiota for complete metabolism into short-chain fatty acids (SCFAs).
30562060	1	23	theme	integral	179:186	arg1	fibers					168:173	Dietary fibers	160:173	Dietary fibers	160:173	Dietary fibers, an integral part of the human diet, require the enzymatic activity of the gut microbiota for complete metabolism into short-chain fatty acids (SCFAs).
30562060	7	24	theme	diet-induced	1540:1551	arg1	obesity					1553:1559	diet-induced obesity	1540:1559	diet-induced obesity	1540:1559	Consistent with the effects on enteroendocrine functions, flaxseed fibers also protected mice from diet-induced obesity, potentially by preventing a reduction in energy expenditure induced by an HFD.
30562060	6	25	from	shifts	1180:1185	arg1	composition					1206:1216	cecal bacterial composition	1190:1216	cecal bacterial composition	1190:1216	The shifts in cecal bacterial composition restored levels of the SCFAs butyrate similar to the chow diet, resulting in colonic but not ileal enteroendocrine cell transcriptional changes in genes related to cell cycle, mRNA, and protein transport compared with HFD.
30562060	6	26	theme	cell	1333:1336	arg1	changes					1354:1360	colonic but not ileal enteroendocrine cell transcriptional changes	1295:1360	colonic but not ileal enteroendocrine cell transcriptional changes in genes related to cell cycle, mRNA, and protein transport	1295:1420	The shifts in cecal bacterial composition restored levels of the SCFAs butyrate similar to the chow diet, resulting in colonic but not ileal enteroendocrine cell transcriptional changes in genes related to cell cycle, mRNA, and protein transport compared with HFD.
30562060	4	27	theme	gut	914:916	arg1	microbiota					918:927	cecal gut microbiota	908:927	cecal gut microbiota	908:927	We fed GPR41-red fluorescent protein mice, an enteroendocrine reporter mouse strain, chow, high-fat diet (HFD), or HFD supplemented either with 10% nonfermentable fiber cellulose or fermentable flaxseed fibers for 12 wk to assess changes in cecal gut microbiota, enteroendocrine cell transcriptome in the ileum and colon, and physiological parameters.
30562060	4	28	theme	high-fat	758:765	arg1	HFD					773:775	HFD	773:775	HFD	773:775	We fed GPR41-red fluorescent protein mice, an enteroendocrine reporter mouse strain, chow, high-fat diet (HFD), or HFD supplemented either with 10% nonfermentable fiber cellulose or fermentable flaxseed fibers for 12 wk to assess changes in cecal gut microbiota, enteroendocrine cell transcriptome in the ileum and colon, and physiological parameters.
30562060	4	28	theme	high-fat	758:765	arg1	diet					767:770	high-fat diet	758:770	high-fat diet (HFD)	758:776	We fed GPR41-red fluorescent protein mice, an enteroendocrine reporter mouse strain, chow, high-fat diet (HFD), or HFD supplemented either with 10% nonfermentable fiber cellulose or fermentable flaxseed fibers for 12 wk to assess changes in cecal gut microbiota, enteroendocrine cell transcriptome in the ileum and colon, and physiological parameters.
30562060	4	28	theme	high-fat	758:765	arg1	strain					744:749	an enteroendocrine reporter mouse strain	710:749	an enteroendocrine reporter mouse strain	710:749	We fed GPR41-red fluorescent protein mice, an enteroendocrine reporter mouse strain, chow, high-fat diet (HFD), or HFD supplemented either with 10% nonfermentable fiber cellulose or fermentable flaxseed fibers for 12 wk to assess changes in cecal gut microbiota, enteroendocrine cell transcriptome in the ileum and colon, and physiological parameters.
30562060	6	29	theme	ileal	1311:1315	arg1	changes					1354:1360	colonic but not ileal enteroendocrine cell transcriptional changes	1295:1360	colonic but not ileal enteroendocrine cell transcriptional changes in genes related to cell cycle, mRNA, and protein transport	1295:1420	The shifts in cecal bacterial composition restored levels of the SCFAs butyrate similar to the chow diet, resulting in colonic but not ileal enteroendocrine cell transcriptional changes in genes related to cell cycle, mRNA, and protein transport compared with HFD.
30562060	5	30	theme	gut	1069:1071	arg1	microbiota					1073:1082	the gut microbiota	1065:1082	the gut microbiota	1065:1082	We observed that flaxseed fibers restructured the gut microbiota and promoted proliferation of the genera Bifidobacterium and Akkermansia compared with HFD.
30562060	5	31	dep	genera	1118:1123	arg1	Bifidobacterium					1125:1139	Bifidobacterium	1125:1139	Bifidobacterium	1125:1139	We observed that flaxseed fibers restructured the gut microbiota and promoted proliferation of the genera Bifidobacterium and Akkermansia compared with HFD.
30562060	5	31	dep	genera	1118:1123	arg1	Akkermansia					1145:1155	Akkermansia	1145:1155	Akkermansia	1145:1155	We observed that flaxseed fibers restructured the gut microbiota and promoted proliferation of the genera Bifidobacterium and Akkermansia compared with HFD.
30562060	0	32	theme	GPR41-expressing	73:88	arg1	cells					106:110	GPR41-expressing enteroendocrine cells	73:110	GPR41-expressing enteroendocrine cells	73:110	Microbial fermentation of flaxseed fibers modulates the transcriptome of GPR41-expressing enteroendocrine cells and protects mice against diet-induced obesity.
30562060	4	33	theme	reporter	729:736	arg1	chow					752:755	chow	752:755	chow	752:755	We fed GPR41-red fluorescent protein mice, an enteroendocrine reporter mouse strain, chow, high-fat diet (HFD), or HFD supplemented either with 10% nonfermentable fiber cellulose or fermentable flaxseed fibers for 12 wk to assess changes in cecal gut microbiota, enteroendocrine cell transcriptome in the ileum and colon, and physiological parameters.
30562060	4	33	theme	reporter	729:736	arg1	diet					767:770	high-fat diet	758:770	high-fat diet (HFD)	758:776	We fed GPR41-red fluorescent protein mice, an enteroendocrine reporter mouse strain, chow, high-fat diet (HFD), or HFD supplemented either with 10% nonfermentable fiber cellulose or fermentable flaxseed fibers for 12 wk to assess changes in cecal gut microbiota, enteroendocrine cell transcriptome in the ileum and colon, and physiological parameters.
30562060	4	33	theme	reporter	729:736	arg1	strain					744:749	an enteroendocrine reporter mouse strain	710:749	an enteroendocrine reporter mouse strain	710:749	We fed GPR41-red fluorescent protein mice, an enteroendocrine reporter mouse strain, chow, high-fat diet (HFD), or HFD supplemented either with 10% nonfermentable fiber cellulose or fermentable flaxseed fibers for 12 wk to assess changes in cecal gut microbiota, enteroendocrine cell transcriptome in the ileum and colon, and physiological parameters.
30562060	4	33	theme	reporter	729:736	arg1	HFD					782:784	HFD	782:784	HFD	782:784	We fed GPR41-red fluorescent protein mice, an enteroendocrine reporter mouse strain, chow, high-fat diet (HFD), or HFD supplemented either with 10% nonfermentable fiber cellulose or fermentable flaxseed fibers for 12 wk to assess changes in cecal gut microbiota, enteroendocrine cell transcriptome in the ileum and colon, and physiological parameters.
30562060	4	33	theme	reporter	729:736	arg1	mice					704:707	GPR41-red fluorescent protein mice	674:707	GPR41-red fluorescent protein mice	674:707	We fed GPR41-red fluorescent protein mice, an enteroendocrine reporter mouse strain, chow, high-fat diet (HFD), or HFD supplemented either with 10% nonfermentable fiber cellulose or fermentable flaxseed fibers for 12 wk to assess changes in cecal gut microbiota, enteroendocrine cell transcriptome in the ileum and colon, and physiological parameters.
30562060	2	34	theme	physiology	381:390	arg1	SCFAs					327:331	SCFAs	327:331	SCFAs	327:331	SCFAs are important modulators of host metabolism and physiology and act in part as signaling molecules by activating G protein-coupled receptors (GPCRs), such as GPR41.
30562060	2	34	theme	physiology	381:390	arg1	modulators					347:356	important modulators	337:356	important modulators of host metabolism and physiology	337:390	SCFAs are important modulators of host metabolism and physiology and act in part as signaling molecules by activating G protein-coupled receptors (GPCRs), such as GPR41.
30562060	0	35	theme	Microbial	0:8	arg1	fermentation					10:21	Microbial fermentation	0:21	Microbial fermentation of flaxseed fibers	0:40	Microbial fermentation of flaxseed fibers modulates the transcriptome of GPR41-expressing enteroendocrine cells and protects mice against diet-induced obesity.
30562060	8	36	theme	favorable	1852:1860	arg1	phenotype					1862:1870	their metabolically favorable phenotype	1832:1870	their metabolically favorable phenotype	1832:1870	Our study shows that flaxseed fibers alter cecal microbial ecology, are fermented to SCFAs in the cecum, and modulate enteroendocrine cell transcriptome in the colon, which may contribute to their metabolically favorable phenotype.
30562060	2	37	theme	signaling	411:419	arg1	molecules					421:429	signaling molecules	411:429	signaling molecules	411:429	SCFAs are important modulators of host metabolism and physiology and act in part as signaling molecules by activating G protein-coupled receptors (GPCRs), such as GPR41.
30562060	2	37	theme	signaling	411:419	arg1	SCFAs					327:331	SCFAs	327:331	SCFAs	327:331	SCFAs are important modulators of host metabolism and physiology and act in part as signaling molecules by activating G protein-coupled receptors (GPCRs), such as GPR41.
30562060	1	38	theme	short-chain	294:304	arg1	SCFAs					319:323	SCFAs	319:323	SCFAs	319:323	Dietary fibers, an integral part of the human diet, require the enzymatic activity of the gut microbiota for complete metabolism into short-chain fatty acids (SCFAs).
30562060	1	38	theme	short-chain	294:304	arg1	acids					312:316	short-chain fatty acids	294:316	short-chain fatty acids (SCFAs)	294:324	Dietary fibers, an integral part of the human diet, require the enzymatic activity of the gut microbiota for complete metabolism into short-chain fatty acids (SCFAs).
30562060	3	39	theme	metabolic	635:643	arg1	benefits					645:652	associated metabolic benefits	624:652	associated metabolic benefits	624:652	Flaxseed fibers improve metabolism in rodents and mice, but their fermentation profiles, effects on enteroendocrine cells, and associated metabolic benefits are unknown.
30562060	0	40	theme	fibers	35:40	arg1	fermentation					10:21	Microbial fermentation	0:21	Microbial fermentation of flaxseed fibers	0:40	Microbial fermentation of flaxseed fibers modulates the transcriptome of GPR41-expressing enteroendocrine cells and protects mice against diet-induced obesity.
30562060	4	41	dep	cellulose	836:844	arg1	either					799:804	either	799:804	either	799:804	We fed GPR41-red fluorescent protein mice, an enteroendocrine reporter mouse strain, chow, high-fat diet (HFD), or HFD supplemented either with 10% nonfermentable fiber cellulose or fermentable flaxseed fibers for 12 wk to assess changes in cecal gut microbiota, enteroendocrine cell transcriptome in the ileum and colon, and physiological parameters.
30562060	2	42	theme	important	337:345	arg1	SCFAs					327:331	SCFAs	327:331	SCFAs	327:331	SCFAs are important modulators of host metabolism and physiology and act in part as signaling molecules by activating G protein-coupled receptors (GPCRs), such as GPR41.
30562060	2	42	theme	important	337:345	arg1	modulators					347:356	important modulators	337:356	important modulators of host metabolism and physiology	337:390	SCFAs are important modulators of host metabolism and physiology and act in part as signaling molecules by activating G protein-coupled receptors (GPCRs), such as GPR41.
30562060	7	43	theme	enteroendocrine	1472:1486	arg1	functions					1488:1496	enteroendocrine functions	1472:1496	enteroendocrine functions	1472:1496	Consistent with the effects on enteroendocrine functions, flaxseed fibers also protected mice from diet-induced obesity, potentially by preventing a reduction in energy expenditure induced by an HFD.
30562060	1	44	theme	enzymatic	224:232	arg1	activity					234:241	the enzymatic activity	220:241	the enzymatic activity of the gut microbiota for complete metabolism into short-chain fatty acids (SCFAs)	220:324	Dietary fibers, an integral part of the human diet, require the enzymatic activity of the gut microbiota for complete metabolism into short-chain fatty acids (SCFAs).
30562060	3	45	from	benefits	645:652	arg1	cells					613:617	enteroendocrine cells	597:617	enteroendocrine cells	597:617	Flaxseed fibers improve metabolism in rodents and mice, but their fermentation profiles, effects on enteroendocrine cells, and associated metabolic benefits are unknown.
30562060	3	46	theme	Flaxseed	497:504	arg1	fibers					506:511	Flaxseed fibers	497:511	Flaxseed fibers	497:511	Flaxseed fibers improve metabolism in rodents and mice, but their fermentation profiles, effects on enteroendocrine cells, and associated metabolic benefits are unknown.
30562060	4	47	dep	ileum	972:976	arg1	the					968:970	the	968:970	the	968:970	We fed GPR41-red fluorescent protein mice, an enteroendocrine reporter mouse strain, chow, high-fat diet (HFD), or HFD supplemented either with 10% nonfermentable fiber cellulose or fermentable flaxseed fibers for 12 wk to assess changes in cecal gut microbiota, enteroendocrine cell transcriptome in the ileum and colon, and physiological parameters.
30562060	7	48	with	Consistent	1441:1450	arg1	effects					1461:1467	the effects	1457:1467	the effects on enteroendocrine functions	1457:1496	Consistent with the effects on enteroendocrine functions, flaxseed fibers also protected mice from diet-induced obesity, potentially by preventing a reduction in energy expenditure induced by an HFD.
30562060	1	49	theme	gut	250:252	arg1	microbiota					254:263	the gut microbiota	246:263	the gut microbiota	246:263	Dietary fibers, an integral part of the human diet, require the enzymatic activity of the gut microbiota for complete metabolism into short-chain fatty acids (SCFAs).
30562060	4	50	theme	nonfermentable	815:828	arg1	cellulose					836:844	10% nonfermentable fiber cellulose	811:844	10% nonfermentable fiber cellulose	811:844	We fed GPR41-red fluorescent protein mice, an enteroendocrine reporter mouse strain, chow, high-fat diet (HFD), or HFD supplemented either with 10% nonfermentable fiber cellulose or fermentable flaxseed fibers for 12 wk to assess changes in cecal gut microbiota, enteroendocrine cell transcriptome in the ileum and colon, and physiological parameters.
30562060	6	51	theme	similar	1256:1262	arg1	levels					1227:1232	levels	1227:1232	levels of the SCFAs butyrate similar to the chow diet	1227:1279	The shifts in cecal bacterial composition restored levels of the SCFAs butyrate similar to the chow diet, resulting in colonic but not ileal enteroendocrine cell transcriptional changes in genes related to cell cycle, mRNA, and protein transport compared with HFD.
30562060	6	52	theme	SCFAs	1241:1245	arg1	butyrate					1247:1254	the SCFAs butyrate	1237:1254	the SCFAs butyrate	1237:1254	The shifts in cecal bacterial composition restored levels of the SCFAs butyrate similar to the chow diet, resulting in colonic but not ileal enteroendocrine cell transcriptional changes in genes related to cell cycle, mRNA, and protein transport compared with HFD.
30562060	8	53	theme	cecal	1684:1688	arg1	ecology					1700:1706	cecal microbial ecology	1684:1706	cecal microbial ecology	1684:1706	Our study shows that flaxseed fibers alter cecal microbial ecology, are fermented to SCFAs in the cecum, and modulate enteroendocrine cell transcriptome in the colon, which may contribute to their metabolically favorable phenotype.
30562060	4	54	theme	10	811:812	arg1	%					813:813	%	813:813	%	813:813	We fed GPR41-red fluorescent protein mice, an enteroendocrine reporter mouse strain, chow, high-fat diet (HFD), or HFD supplemented either with 10% nonfermentable fiber cellulose or fermentable flaxseed fibers for 12 wk to assess changes in cecal gut microbiota, enteroendocrine cell transcriptome in the ileum and colon, and physiological parameters.
30562060	1	55	theme	diet	206:209	arg1	part					188:191	an integral part	176:191	an integral part of the human diet	176:209	Dietary fibers, an integral part of the human diet, require the enzymatic activity of the gut microbiota for complete metabolism into short-chain fatty acids (SCFAs).
30562060	1	55	theme	diet	206:209	arg1	fibers					168:173	Dietary fibers	160:173	Dietary fibers	160:173	Dietary fibers, an integral part of the human diet, require the enzymatic activity of the gut microbiota for complete metabolism into short-chain fatty acids (SCFAs).
30562060	7	56	theme	energy	1603:1608	arg1	expenditure					1610:1620	energy expenditure	1603:1620	energy expenditure induced by an HFD	1603:1638	Consistent with the effects on enteroendocrine functions, flaxseed fibers also protected mice from diet-induced obesity, potentially by preventing a reduction in energy expenditure induced by an HFD.
30562060	6	57	theme	colonic	1295:1301	arg1	changes					1354:1360	colonic but not ileal enteroendocrine cell transcriptional changes	1295:1360	colonic but not ileal enteroendocrine cell transcriptional changes in genes related to cell cycle, mRNA, and protein transport	1295:1420	The shifts in cecal bacterial composition restored levels of the SCFAs butyrate similar to the chow diet, resulting in colonic but not ileal enteroendocrine cell transcriptional changes in genes related to cell cycle, mRNA, and protein transport compared with HFD.
30562060	4	58	theme	fermentable	849:859	arg1	fibers					870:875	fermentable flaxseed fibers	849:875	fermentable flaxseed fibers	849:875	We fed GPR41-red fluorescent protein mice, an enteroendocrine reporter mouse strain, chow, high-fat diet (HFD), or HFD supplemented either with 10% nonfermentable fiber cellulose or fermentable flaxseed fibers for 12 wk to assess changes in cecal gut microbiota, enteroendocrine cell transcriptome in the ileum and colon, and physiological parameters.
30562060	6	59	theme	cecal	1190:1194	arg1	composition					1206:1216	cecal bacterial composition	1190:1216	cecal bacterial composition	1190:1216	The shifts in cecal bacterial composition restored levels of the SCFAs butyrate similar to the chow diet, resulting in colonic but not ileal enteroendocrine cell transcriptional changes in genes related to cell cycle, mRNA, and protein transport compared with HFD.
30562060	4	60	theme	protein	696:702	arg1	mice					704:707	GPR41-red fluorescent protein mice	674:707	GPR41-red fluorescent protein mice	674:707	We fed GPR41-red fluorescent protein mice, an enteroendocrine reporter mouse strain, chow, high-fat diet (HFD), or HFD supplemented either with 10% nonfermentable fiber cellulose or fermentable flaxseed fibers for 12 wk to assess changes in cecal gut microbiota, enteroendocrine cell transcriptome in the ileum and colon, and physiological parameters.
30562060	4	60	theme	protein	696:702	arg1	strain					744:749	an enteroendocrine reporter mouse strain	710:749	an enteroendocrine reporter mouse strain	710:749	We fed GPR41-red fluorescent protein mice, an enteroendocrine reporter mouse strain, chow, high-fat diet (HFD), or HFD supplemented either with 10% nonfermentable fiber cellulose or fermentable flaxseed fibers for 12 wk to assess changes in cecal gut microbiota, enteroendocrine cell transcriptome in the ileum and colon, and physiological parameters.
30562060	3	61	theme	enteroendocrine	597:611	arg1	cells					613:617	enteroendocrine cells	597:617	enteroendocrine cells	597:617	Flaxseed fibers improve metabolism in rodents and mice, but their fermentation profiles, effects on enteroendocrine cells, and associated metabolic benefits are unknown.
30562060	6	62	theme	related	1371:1377	arg1	genes					1365:1369	genes	1365:1369	genes related to cell cycle, mRNA, and protein transport	1365:1420	The shifts in cecal bacterial composition restored levels of the SCFAs butyrate similar to the chow diet, resulting in colonic but not ileal enteroendocrine cell transcriptional changes in genes related to cell cycle, mRNA, and protein transport compared with HFD.
30562060	0	63	theme	cells	106:110	arg1	transcriptome					56:68	the transcriptome	52:68	the transcriptome of GPR41-expressing enteroendocrine cells	52:110	Microbial fermentation of flaxseed fibers modulates the transcriptome of GPR41-expressing enteroendocrine cells and protects mice against diet-induced obesity.
30562060	6	64	from	changes	1354:1360	arg1	genes					1365:1369	genes	1365:1369	genes related to cell cycle, mRNA, and protein transport	1365:1420	The shifts in cecal bacterial composition restored levels of the SCFAs butyrate similar to the chow diet, resulting in colonic but not ileal enteroendocrine cell transcriptional changes in genes related to cell cycle, mRNA, and protein transport compared with HFD.
30562060	4	65	from	parameters	1007:1016	arg1	colon					982:986	colon	982:986	colon	982:986	We fed GPR41-red fluorescent protein mice, an enteroendocrine reporter mouse strain, chow, high-fat diet (HFD), or HFD supplemented either with 10% nonfermentable fiber cellulose or fermentable flaxseed fibers for 12 wk to assess changes in cecal gut microbiota, enteroendocrine cell transcriptome in the ileum and colon, and physiological parameters.
30562060	4	65	from	parameters	1007:1016	arg1	ileum					972:976	ileum	972:976	ileum	972:976	We fed GPR41-red fluorescent protein mice, an enteroendocrine reporter mouse strain, chow, high-fat diet (HFD), or HFD supplemented either with 10% nonfermentable fiber cellulose or fermentable flaxseed fibers for 12 wk to assess changes in cecal gut microbiota, enteroendocrine cell transcriptome in the ileum and colon, and physiological parameters.
30562060	4	66	theme	enteroendocrine	930:944	arg1	transcriptome					951:963	enteroendocrine cell transcriptome	930:963	enteroendocrine cell transcriptome in the ileum and colon	930:986	We fed GPR41-red fluorescent protein mice, an enteroendocrine reporter mouse strain, chow, high-fat diet (HFD), or HFD supplemented either with 10% nonfermentable fiber cellulose or fermentable flaxseed fibers for 12 wk to assess changes in cecal gut microbiota, enteroendocrine cell transcriptome in the ileum and colon, and physiological parameters.
30562060	4	67	theme	GPR41-red	674:682	arg1	mice					704:707	GPR41-red fluorescent protein mice	674:707	GPR41-red fluorescent protein mice	674:707	We fed GPR41-red fluorescent protein mice, an enteroendocrine reporter mouse strain, chow, high-fat diet (HFD), or HFD supplemented either with 10% nonfermentable fiber cellulose or fermentable flaxseed fibers for 12 wk to assess changes in cecal gut microbiota, enteroendocrine cell transcriptome in the ileum and colon, and physiological parameters.
30562060	4	67	theme	GPR41-red	674:682	arg1	strain					744:749	an enteroendocrine reporter mouse strain	710:749	an enteroendocrine reporter mouse strain	710:749	We fed GPR41-red fluorescent protein mice, an enteroendocrine reporter mouse strain, chow, high-fat diet (HFD), or HFD supplemented either with 10% nonfermentable fiber cellulose or fermentable flaxseed fibers for 12 wk to assess changes in cecal gut microbiota, enteroendocrine cell transcriptome in the ileum and colon, and physiological parameters.
30562060	6	68	theme	transcriptional	1338:1352	arg1	changes					1354:1360	colonic but not ileal enteroendocrine cell transcriptional changes	1295:1360	colonic but not ileal enteroendocrine cell transcriptional changes in genes related to cell cycle, mRNA, and protein transport	1295:1420	The shifts in cecal bacterial composition restored levels of the SCFAs butyrate similar to the chow diet, resulting in colonic but not ileal enteroendocrine cell transcriptional changes in genes related to cell cycle, mRNA, and protein transport compared with HFD.
30562060	7	69	from	reduction	1590:1598	arg1	expenditure					1610:1620	energy expenditure	1603:1620	energy expenditure induced by an HFD	1603:1638	Consistent with the effects on enteroendocrine functions, flaxseed fibers also protected mice from diet-induced obesity, potentially by preventing a reduction in energy expenditure induced by an HFD.
30562060	8	70	theme	enteroendocrine	1759:1773	arg1	transcriptome					1780:1792	enteroendocrine cell transcriptome	1759:1792	enteroendocrine cell transcriptome	1759:1792	Our study shows that flaxseed fibers alter cecal microbial ecology, are fermented to SCFAs in the cecum, and modulate enteroendocrine cell transcriptome in the colon, which may contribute to their metabolically favorable phenotype.
30562060	3	71	from	effects	586:592	arg1	cells					613:617	enteroendocrine cells	597:617	enteroendocrine cells	597:617	Flaxseed fibers improve metabolism in rodents and mice, but their fermentation profiles, effects on enteroendocrine cells, and associated metabolic benefits are unknown.
30562060	6	72	theme	enteroendocrine	1317:1331	arg1	changes					1354:1360	colonic but not ileal enteroendocrine cell transcriptional changes	1295:1360	colonic but not ileal enteroendocrine cell transcriptional changes in genes related to cell cycle, mRNA, and protein transport	1295:1420	The shifts in cecal bacterial composition restored levels of the SCFAs butyrate similar to the chow diet, resulting in colonic but not ileal enteroendocrine cell transcriptional changes in genes related to cell cycle, mRNA, and protein transport compared with HFD.
30562060	4	73	theme	cecal	908:912	arg1	microbiota					918:927	cecal gut microbiota	908:927	cecal gut microbiota	908:927	We fed GPR41-red fluorescent protein mice, an enteroendocrine reporter mouse strain, chow, high-fat diet (HFD), or HFD supplemented either with 10% nonfermentable fiber cellulose or fermentable flaxseed fibers for 12 wk to assess changes in cecal gut microbiota, enteroendocrine cell transcriptome in the ileum and colon, and physiological parameters.
30562060	6	74	theme	protein	1404:1410	arg1	transport					1412:1420	protein transport	1404:1420	protein transport	1404:1420	The shifts in cecal bacterial composition restored levels of the SCFAs butyrate similar to the chow diet, resulting in colonic but not ileal enteroendocrine cell transcriptional changes in genes related to cell cycle, mRNA, and protein transport compared with HFD.
30562060	4	75	theme	enteroendocrine	713:727	arg1	chow					752:755	chow	752:755	chow	752:755	We fed GPR41-red fluorescent protein mice, an enteroendocrine reporter mouse strain, chow, high-fat diet (HFD), or HFD supplemented either with 10% nonfermentable fiber cellulose or fermentable flaxseed fibers for 12 wk to assess changes in cecal gut microbiota, enteroendocrine cell transcriptome in the ileum and colon, and physiological parameters.
30562060	4	75	theme	enteroendocrine	713:727	arg1	diet					767:770	high-fat diet	758:770	high-fat diet (HFD)	758:776	We fed GPR41-red fluorescent protein mice, an enteroendocrine reporter mouse strain, chow, high-fat diet (HFD), or HFD supplemented either with 10% nonfermentable fiber cellulose or fermentable flaxseed fibers for 12 wk to assess changes in cecal gut microbiota, enteroendocrine cell transcriptome in the ileum and colon, and physiological parameters.
30562060	4	75	theme	enteroendocrine	713:727	arg1	strain					744:749	an enteroendocrine reporter mouse strain	710:749	an enteroendocrine reporter mouse strain	710:749	We fed GPR41-red fluorescent protein mice, an enteroendocrine reporter mouse strain, chow, high-fat diet (HFD), or HFD supplemented either with 10% nonfermentable fiber cellulose or fermentable flaxseed fibers for 12 wk to assess changes in cecal gut microbiota, enteroendocrine cell transcriptome in the ileum and colon, and physiological parameters.
30562060	4	75	theme	enteroendocrine	713:727	arg1	HFD					782:784	HFD	782:784	HFD	782:784	We fed GPR41-red fluorescent protein mice, an enteroendocrine reporter mouse strain, chow, high-fat diet (HFD), or HFD supplemented either with 10% nonfermentable fiber cellulose or fermentable flaxseed fibers for 12 wk to assess changes in cecal gut microbiota, enteroendocrine cell transcriptome in the ileum and colon, and physiological parameters.
30562060	4	75	theme	enteroendocrine	713:727	arg1	mice					704:707	GPR41-red fluorescent protein mice	674:707	GPR41-red fluorescent protein mice	674:707	We fed GPR41-red fluorescent protein mice, an enteroendocrine reporter mouse strain, chow, high-fat diet (HFD), or HFD supplemented either with 10% nonfermentable fiber cellulose or fermentable flaxseed fibers for 12 wk to assess changes in cecal gut microbiota, enteroendocrine cell transcriptome in the ileum and colon, and physiological parameters.
30562060	0	76	theme	flaxseed	26:33	arg1	fibers					35:40	flaxseed fibers	26:40	flaxseed fibers	26:40	Microbial fermentation of flaxseed fibers modulates the transcriptome of GPR41-expressing enteroendocrine cells and protects mice against diet-induced obesity.
30562060	3	77	theme	associated	624:633	arg1	benefits					645:652	associated metabolic benefits	624:652	associated metabolic benefits	624:652	Flaxseed fibers improve metabolism in rodents and mice, but their fermentation profiles, effects on enteroendocrine cells, and associated metabolic benefits are unknown.
30562060	4	78	theme	mouse	738:742	arg1	chow					752:755	chow	752:755	chow	752:755	We fed GPR41-red fluorescent protein mice, an enteroendocrine reporter mouse strain, chow, high-fat diet (HFD), or HFD supplemented either with 10% nonfermentable fiber cellulose or fermentable flaxseed fibers for 12 wk to assess changes in cecal gut microbiota, enteroendocrine cell transcriptome in the ileum and colon, and physiological parameters.
30562060	4	78	theme	mouse	738:742	arg1	diet					767:770	high-fat diet	758:770	high-fat diet (HFD)	758:776	We fed GPR41-red fluorescent protein mice, an enteroendocrine reporter mouse strain, chow, high-fat diet (HFD), or HFD supplemented either with 10% nonfermentable fiber cellulose or fermentable flaxseed fibers for 12 wk to assess changes in cecal gut microbiota, enteroendocrine cell transcriptome in the ileum and colon, and physiological parameters.
30562060	4	78	theme	mouse	738:742	arg1	strain					744:749	an enteroendocrine reporter mouse strain	710:749	an enteroendocrine reporter mouse strain	710:749	We fed GPR41-red fluorescent protein mice, an enteroendocrine reporter mouse strain, chow, high-fat diet (HFD), or HFD supplemented either with 10% nonfermentable fiber cellulose or fermentable flaxseed fibers for 12 wk to assess changes in cecal gut microbiota, enteroendocrine cell transcriptome in the ileum and colon, and physiological parameters.
30562060	4	78	theme	mouse	738:742	arg1	HFD					782:784	HFD	782:784	HFD	782:784	We fed GPR41-red fluorescent protein mice, an enteroendocrine reporter mouse strain, chow, high-fat diet (HFD), or HFD supplemented either with 10% nonfermentable fiber cellulose or fermentable flaxseed fibers for 12 wk to assess changes in cecal gut microbiota, enteroendocrine cell transcriptome in the ileum and colon, and physiological parameters.
30562060	4	78	theme	mouse	738:742	arg1	mice					704:707	GPR41-red fluorescent protein mice	674:707	GPR41-red fluorescent protein mice	674:707	We fed GPR41-red fluorescent protein mice, an enteroendocrine reporter mouse strain, chow, high-fat diet (HFD), or HFD supplemented either with 10% nonfermentable fiber cellulose or fermentable flaxseed fibers for 12 wk to assess changes in cecal gut microbiota, enteroendocrine cell transcriptome in the ileum and colon, and physiological parameters.
30562060	3	79	from	profiles	576:583	arg1	cells					613:617	enteroendocrine cells	597:617	enteroendocrine cells	597:617	Flaxseed fibers improve metabolism in rodents and mice, but their fermentation profiles, effects on enteroendocrine cells, and associated metabolic benefits are unknown.
30562060	2	80	theme	metabolism	366:375	arg1	SCFAs					327:331	SCFAs	327:331	SCFAs	327:331	SCFAs are important modulators of host metabolism and physiology and act in part as signaling molecules by activating G protein-coupled receptors (GPCRs), such as GPR41.
30562060	2	80	theme	metabolism	366:375	arg1	modulators					347:356	important modulators	337:356	important modulators of host metabolism and physiology	337:390	SCFAs are important modulators of host metabolism and physiology and act in part as signaling molecules by activating G protein-coupled receptors (GPCRs), such as GPR41.
30562060	1	81	theme	fatty	306:310	arg1	SCFAs					319:323	SCFAs	319:323	SCFAs	319:323	Dietary fibers, an integral part of the human diet, require the enzymatic activity of the gut microbiota for complete metabolism into short-chain fatty acids (SCFAs).
30562060	1	81	theme	fatty	306:310	arg1	acids					312:316	short-chain fatty acids	294:316	short-chain fatty acids (SCFAs)	294:324	Dietary fibers, an integral part of the human diet, require the enzymatic activity of the gut microbiota for complete metabolism into short-chain fatty acids (SCFAs).
30562060	6	82	theme	cell	1382:1385	arg1	cycle					1387:1391	cell cycle	1382:1391	cell cycle	1382:1391	The shifts in cecal bacterial composition restored levels of the SCFAs butyrate similar to the chow diet, resulting in colonic but not ileal enteroendocrine cell transcriptional changes in genes related to cell cycle, mRNA, and protein transport compared with HFD.
30562060	5	83	theme	genera	1118:1123	arg1	proliferation					1097:1109	proliferation	1097:1109	proliferation of the genera Bifidobacterium and Akkermansia	1097:1155	We observed that flaxseed fibers restructured the gut microbiota and promoted proliferation of the genera Bifidobacterium and Akkermansia compared with HFD.
30562060	7	84	theme	flaxseed	1499:1506	arg1	fibers					1508:1513	flaxseed fibers	1499:1513	flaxseed fibers	1499:1513	Consistent with the effects on enteroendocrine functions, flaxseed fibers also protected mice from diet-induced obesity, potentially by preventing a reduction in energy expenditure induced by an HFD.
30562060	2	85	theme	protein-coupled	447:461	arg1	receptors					463:471	G protein-coupled receptors	445:471	G protein-coupled receptors (GPCRs)	445:479	SCFAs are important modulators of host metabolism and physiology and act in part as signaling molecules by activating G protein-coupled receptors (GPCRs), such as GPR41.
30562060	2	85	theme	protein-coupled	447:461	arg1	GPR41					490:494	GPR41	490:494	GPR41	490:494	SCFAs are important modulators of host metabolism and physiology and act in part as signaling molecules by activating G protein-coupled receptors (GPCRs), such as GPR41.
30562060	2	85	theme	protein-coupled	447:461	arg1	GPCRs					474:478	GPCRs	474:478	GPCRs	474:478	SCFAs are important modulators of host metabolism and physiology and act in part as signaling molecules by activating G protein-coupled receptors (GPCRs), such as GPR41.
30562060	4	86	from	transcriptome	951:963	arg1	colon					982:986	colon	982:986	colon	982:986	We fed GPR41-red fluorescent protein mice, an enteroendocrine reporter mouse strain, chow, high-fat diet (HFD), or HFD supplemented either with 10% nonfermentable fiber cellulose or fermentable flaxseed fibers for 12 wk to assess changes in cecal gut microbiota, enteroendocrine cell transcriptome in the ileum and colon, and physiological parameters.
30562060	4	86	from	transcriptome	951:963	arg1	ileum					972:976	ileum	972:976	ileum	972:976	We fed GPR41-red fluorescent protein mice, an enteroendocrine reporter mouse strain, chow, high-fat diet (HFD), or HFD supplemented either with 10% nonfermentable fiber cellulose or fermentable flaxseed fibers for 12 wk to assess changes in cecal gut microbiota, enteroendocrine cell transcriptome in the ileum and colon, and physiological parameters.
30562060	7	87	from	effects	1461:1467	arg1	functions					1488:1496	enteroendocrine functions	1472:1496	enteroendocrine functions	1472:1496	Consistent with the effects on enteroendocrine functions, flaxseed fibers also protected mice from diet-induced obesity, potentially by preventing a reduction in energy expenditure induced by an HFD.
30562060	2	88	theme	host	361:364	arg1	metabolism					366:375	host metabolism	361:375	host metabolism	361:375	SCFAs are important modulators of host metabolism and physiology and act in part as signaling molecules by activating G protein-coupled receptors (GPCRs), such as GPR41.
30562060	4	89	from	microbiota	918:927	arg1	colon					982:986	colon	982:986	colon	982:986	We fed GPR41-red fluorescent protein mice, an enteroendocrine reporter mouse strain, chow, high-fat diet (HFD), or HFD supplemented either with 10% nonfermentable fiber cellulose or fermentable flaxseed fibers for 12 wk to assess changes in cecal gut microbiota, enteroendocrine cell transcriptome in the ileum and colon, and physiological parameters.
30562060	4	89	from	microbiota	918:927	arg1	ileum					972:976	ileum	972:976	ileum	972:976	We fed GPR41-red fluorescent protein mice, an enteroendocrine reporter mouse strain, chow, high-fat diet (HFD), or HFD supplemented either with 10% nonfermentable fiber cellulose or fermentable flaxseed fibers for 12 wk to assess changes in cecal gut microbiota, enteroendocrine cell transcriptome in the ileum and colon, and physiological parameters.
30498207	2	0	from	differences	398:408	arg1	microbiota					421:430	the gut microbiota and plasma lipopolysaccharide level	413:466	microbiota	421:430	In this study, to increase our understanding of the potential cause of the high rate of infection in postsplenectomy patients, we evaluated the differences in the gut microbiota and plasma lipopolysaccharide level of patients after splenectomy relative to those of healthy controls.
30498207	2	0	from	differences	398:408	arg1	level					462:466	the gut microbiota and plasma lipopolysaccharide level	413:466	level	462:466	In this study, to increase our understanding of the potential cause of the high rate of infection in postsplenectomy patients, we evaluated the differences in the gut microbiota and plasma lipopolysaccharide level of patients after splenectomy relative to those of healthy controls.
30498207	1	1	theme	chronic	156:162	arg1	inflammation					175:186	the chronic, low-grade inflammation	152:186	inflammation	175:186	Splenectomy carries a long-term risk of postoperative infection, and the chronic, low-grade inflammation associated with endotoxemia may be related to the gut microbiota.
30498207	1	1	theme	chronic	156:162	arg1	related					223:229	related	223:229	related	223:229	Splenectomy carries a long-term risk of postoperative infection, and the chronic, low-grade inflammation associated with endotoxemia may be related to the gut microbiota.
30498207	6	2	theme	gut	1004:1006	arg1	composition					1019:1029	gut microbiota composition	1004:1029	gut microbiota composition	1004:1029	Significant differences were observed in gut microbiota composition with regard to the relative bacterial abundances of 2 phyla, 7 families, and 15 genera.
30498207	2	3	theme	patients	471:478	arg1	microbiota					421:430	the gut microbiota and plasma lipopolysaccharide level	413:466	microbiota	421:430	In this study, to increase our understanding of the potential cause of the high rate of infection in postsplenectomy patients, we evaluated the differences in the gut microbiota and plasma lipopolysaccharide level of patients after splenectomy relative to those of healthy controls.
30498207	2	3	theme	patients	471:478	arg1	level					462:466	the gut microbiota and plasma lipopolysaccharide level	413:466	level	462:466	In this study, to increase our understanding of the potential cause of the high rate of infection in postsplenectomy patients, we evaluated the differences in the gut microbiota and plasma lipopolysaccharide level of patients after splenectomy relative to those of healthy controls.
30498207	6	4	theme	families	1094:1101	arg1	abundances					1069:1078	the relative bacterial abundances	1046:1078	the relative bacterial abundances of 2 phyla, 7 families, and 15 genera	1046:1116	Significant differences were observed in gut microbiota composition with regard to the relative bacterial abundances of 2 phyla, 7 families, and 15 genera.
30498207	2	5	theme	rate	334:337	arg1	cause					316:320	the potential cause	302:320	the potential cause of the high rate of infection in postsplenectomy patients	302:378	In this study, to increase our understanding of the potential cause of the high rate of infection in postsplenectomy patients, we evaluated the differences in the gut microbiota and plasma lipopolysaccharide level of patients after splenectomy relative to those of healthy controls.
30498207	9	6	theme	biosynthesis	1478:1489	arg1	proteins					1491:1498	lipopolysaccharide biosynthesis proteins	1459:1498	lipopolysaccharide biosynthesis proteins	1459:1498	The PICRUSt analysis showed that the relative abundances of lipopolysaccharide biosynthesis proteins and lipopolysaccharide biosynthesis pathways were higher in the SP group and were positively associated with the plasma lipopolysaccharide level.
30498207	2	7	theme	healthy	519:525	arg1	controls					527:534	healthy controls	519:534	healthy controls	519:534	In this study, to increase our understanding of the potential cause of the high rate of infection in postsplenectomy patients, we evaluated the differences in the gut microbiota and plasma lipopolysaccharide level of patients after splenectomy relative to those of healthy controls.
30498207	8	8	theme	time	1393:1396	arg1	length					1365:1370	the length	1361:1370	the length of the postoperative time	1361:1396	The degree of the microbiota alteration increased with the length of the postoperative time.
30498207	2	9	from	cause	316:320	arg1	patients					371:378	postsplenectomy patients	355:378	postsplenectomy patients	355:378	In this study, to increase our understanding of the potential cause of the high rate of infection in postsplenectomy patients, we evaluated the differences in the gut microbiota and plasma lipopolysaccharide level of patients after splenectomy relative to those of healthy controls.
30498207	5	10	theme	gene	867:870	arg1	sequencing					872:881	rRNA gene sequencing	862:881	rRNA gene sequencing	862:881	Fecal samples were used for gut microbiota analysis via 16s rRNA gene sequencing, blood examinations and plasma lipopolysaccharide measurements were also taken.
30498207	9	11	theme	SP	1564:1565	arg1	group					1567:1571	the SP group	1560:1571	the SP group	1560:1571	The PICRUSt analysis showed that the relative abundances of lipopolysaccharide biosynthesis proteins and lipopolysaccharide biosynthesis pathways were higher in the SP group and were positively associated with the plasma lipopolysaccharide level.
30498207	5	12	theme	lipopolysaccharide	914:931	arg1	measurements					933:944	plasma lipopolysaccharide measurements	907:944	plasma lipopolysaccharide measurements	907:944	Fecal samples were used for gut microbiota analysis via 16s rRNA gene sequencing, blood examinations and plasma lipopolysaccharide measurements were also taken.
30498207	9	13	theme	lipopolysaccharide	1504:1521	arg1	pathways					1536:1543	lipopolysaccharide biosynthesis pathways	1504:1543	lipopolysaccharide biosynthesis pathways	1504:1543	The PICRUSt analysis showed that the relative abundances of lipopolysaccharide biosynthesis proteins and lipopolysaccharide biosynthesis pathways were higher in the SP group and were positively associated with the plasma lipopolysaccharide level.
30498207	11	14	theme	increased	1893:1901	arg1	risk					1903:1906	an increased risk	1890:1906	an increased risk of complications	1890:1923	Further studies are needed to verify whether such alterations after splenectomy are related to an increased risk of complications.
30498207	11	15	theme	complications	1911:1923	arg1	risk					1903:1906	an increased risk	1890:1906	an increased risk of complications	1890:1923	Further studies are needed to verify whether such alterations after splenectomy are related to an increased risk of complications.
30498207	9	16	theme	relative	1436:1443	arg1	abundances					1445:1454	the relative abundances	1432:1454	the relative abundances of lipopolysaccharide biosynthesis proteins and lipopolysaccharide biosynthesis pathways	1432:1543	The PICRUSt analysis showed that the relative abundances of lipopolysaccharide biosynthesis proteins and lipopolysaccharide biosynthesis pathways were higher in the SP group and were positively associated with the plasma lipopolysaccharide level.
30498207	9	16	theme	relative	1436:1443	arg1	higher					1550:1555	higher	1550:1555	higher	1550:1555	The PICRUSt analysis showed that the relative abundances of lipopolysaccharide biosynthesis proteins and lipopolysaccharide biosynthesis pathways were higher in the SP group and were positively associated with the plasma lipopolysaccharide level.
30498207	4	17	theme	SP	704:705	arg1	group					707:711	The SP group	700:711	The SP group	700:711	The SP group was subdivided into three subgroups according to the length of their postoperative time.
30498207	7	18	theme	SP	1180:1181	arg1	group					1183:1187	the SP group	1176:1187	the SP group	1176:1187	The lipopolysaccharide level was significantly higher in the SP group than in the HC group and were negatively associated with five bacterial families with low abundance in the SP group.
30498207	5	19	theme	blood	884:888	arg1	examinations					890:901	blood examinations	884:901	blood examinations	884:901	Fecal samples were used for gut microbiota analysis via 16s rRNA gene sequencing, blood examinations and plasma lipopolysaccharide measurements were also taken.
30498207	6	20	theme	phyla	1085:1089	arg1	abundances					1069:1078	the relative bacterial abundances	1046:1078	the relative bacterial abundances of 2 phyla, 7 families, and 15 genera	1046:1116	Significant differences were observed in gut microbiota composition with regard to the relative bacterial abundances of 2 phyla, 7 families, and 15 genera.
30498207	5	21	used	used	821:824	arg2	samples					808:814	Fecal samples	802:814	Fecal samples	802:814	Fecal samples were used for gut microbiota analysis via 16s rRNA gene sequencing, blood examinations and plasma lipopolysaccharide measurements were also taken.
30498207	1	22	theme	low-grade	165:173	arg1	inflammation					175:186	the chronic, low-grade inflammation	152:186	inflammation	175:186	Splenectomy carries a long-term risk of postoperative infection, and the chronic, low-grade inflammation associated with endotoxemia may be related to the gut microbiota.
30498207	1	22	theme	low-grade	165:173	arg1	related					223:229	related	223:229	related	223:229	Splenectomy carries a long-term risk of postoperative infection, and the chronic, low-grade inflammation associated with endotoxemia may be related to the gut microbiota.
30498207	7	23	theme	lipopolysaccharide	1123:1140	arg1	level					1142:1146	The lipopolysaccharide level	1119:1146	The lipopolysaccharide level	1119:1146	The lipopolysaccharide level was significantly higher in the SP group than in the HC group and were negatively associated with five bacterial families with low abundance in the SP group.
30498207	7	23	theme	lipopolysaccharide	1123:1140	arg1	higher					1166:1171	higher	1166:1171	higher	1166:1171	The lipopolysaccharide level was significantly higher in the SP group than in the HC group and were negatively associated with five bacterial families with low abundance in the SP group.
30498207	6	24	theme	relative	1050:1057	arg1	abundances					1069:1078	the relative bacterial abundances	1046:1078	the relative bacterial abundances of 2 phyla, 7 families, and 15 genera	1046:1116	Significant differences were observed in gut microbiota composition with regard to the relative bacterial abundances of 2 phyla, 7 families, and 15 genera.
30498207	6	25	located	observed	992:999	arg2	differences					975:985	Significant differences	963:985	Significant differences	963:985	Significant differences were observed in gut microbiota composition with regard to the relative bacterial abundances of 2 phyla, 7 families, and 15 genera.
30498207	6	25	located	observed	992:999	arg1	composition					1019:1029	gut microbiota composition	1004:1029	gut microbiota composition	1004:1029	Significant differences were observed in gut microbiota composition with regard to the relative bacterial abundances of 2 phyla, 7 families, and 15 genera.
30498207	0	26	theme	gut	8:10	arg1	microbiota					12:21	Altered gut microbiota	0:21	Altered gut microbiota after traumatic splenectomy	0:49	Altered gut microbiota after traumatic splenectomy is associated with endotoxemia.
30498207	7	27	theme	HC	1201:1202	arg1	group					1204:1208	the HC group	1197:1208	the HC group	1197:1208	The lipopolysaccharide level was significantly higher in the SP group than in the HC group and were negatively associated with five bacterial families with low abundance in the SP group.
30498207	7	28	with	families	1261:1268	arg1	abundance					1279:1287	low abundance	1275:1287	low abundance in the SP group	1275:1303	The lipopolysaccharide level was significantly higher in the SP group than in the HC group and were negatively associated with five bacterial families with low abundance in the SP group.
30498207	2	29	from	rate	334:337	arg1	patients					371:378	postsplenectomy patients	355:378	postsplenectomy patients	355:378	In this study, to increase our understanding of the potential cause of the high rate of infection in postsplenectomy patients, we evaluated the differences in the gut microbiota and plasma lipopolysaccharide level of patients after splenectomy relative to those of healthy controls.
30498207	2	30	theme	potential	306:314	arg1	cause					316:320	the potential cause	302:320	the potential cause of the high rate of infection in postsplenectomy patients	302:378	In this study, to increase our understanding of the potential cause of the high rate of infection in postsplenectomy patients, we evaluated the differences in the gut microbiota and plasma lipopolysaccharide level of patients after splenectomy relative to those of healthy controls.
30498207	7	31	theme	bacterial	1251:1259	arg1	families					1261:1268	five bacterial families	1246:1268	five bacterial families with low abundance in the SP group	1246:1303	The lipopolysaccharide level was significantly higher in the SP group than in the HC group and were negatively associated with five bacterial families with low abundance in the SP group.
30498207	7	32	theme	SP	1296:1297	arg1	group					1299:1303	the SP group	1292:1303	the SP group	1292:1303	The lipopolysaccharide level was significantly higher in the SP group than in the HC group and were negatively associated with five bacterial families with low abundance in the SP group.
30498207	0	33	theme	Altered	0:6	arg1	microbiota					12:21	Altered gut microbiota	0:21	Altered gut microbiota after traumatic splenectomy	0:49	Altered gut microbiota after traumatic splenectomy is associated with endotoxemia.
30498207	10	34	located	observed	1675:1682	arg2	alterations					1658:1668	Significant alterations	1646:1668	Significant alterations	1646:1668	Significant alterations were observed in the gut microbiota of the splenectomized patients and were associated with plasma lipopolysaccharide level.
30498207	10	34	located	observed	1675:1682	arg1	microbiota					1695:1704	the gut microbiota	1687:1704	the gut microbiota of the splenectomized patients	1687:1735	Significant alterations were observed in the gut microbiota of the splenectomized patients and were associated with plasma lipopolysaccharide level.
30498207	5	35	theme	microbiota	834:843	arg1	analysis					845:852	gut microbiota analysis	830:852	gut microbiota analysis	830:852	Fecal samples were used for gut microbiota analysis via 16s rRNA gene sequencing, blood examinations and plasma lipopolysaccharide measurements were also taken.
30498207	8	36	theme	alteration	1335:1344	arg1	degree					1310:1315	The degree	1306:1315	The degree of the microbiota alteration	1306:1344	The degree of the microbiota alteration increased with the length of the postoperative time.
30498207	2	37	theme	postsplenectomy	355:369	arg1	patients					371:378	postsplenectomy patients	355:378	postsplenectomy patients	355:378	In this study, to increase our understanding of the potential cause of the high rate of infection in postsplenectomy patients, we evaluated the differences in the gut microbiota and plasma lipopolysaccharide level of patients after splenectomy relative to those of healthy controls.
30498207	2	38	theme	gut	417:419	arg1	microbiota					421:430	the gut microbiota and plasma lipopolysaccharide level	413:466	microbiota	421:430	In this study, to increase our understanding of the potential cause of the high rate of infection in postsplenectomy patients, we evaluated the differences in the gut microbiota and plasma lipopolysaccharide level of patients after splenectomy relative to those of healthy controls.
30498207	6	39	theme	Significant	963:973	arg1	differences					975:985	Significant differences	963:985	Significant differences	963:985	Significant differences were observed in gut microbiota composition with regard to the relative bacterial abundances of 2 phyla, 7 families, and 15 genera.
30498207	5	40	theme	plasma	907:912	arg1	measurements					933:944	plasma lipopolysaccharide measurements	907:944	plasma lipopolysaccharide measurements	907:944	Fecal samples were used for gut microbiota analysis via 16s rRNA gene sequencing, blood examinations and plasma lipopolysaccharide measurements were also taken.
30498207	1	41	theme	long-term	105:113	arg1	risk					115:118	a long-term risk	103:118	a long-term risk of postoperative infection	103:145	Splenectomy carries a long-term risk of postoperative infection, and the chronic, low-grade inflammation associated with endotoxemia may be related to the gut microbiota.
30498207	6	42	theme	bacterial	1059:1067	arg1	abundances					1069:1078	the relative bacterial abundances	1046:1078	the relative bacterial abundances of 2 phyla, 7 families, and 15 genera	1046:1116	Significant differences were observed in gut microbiota composition with regard to the relative bacterial abundances of 2 phyla, 7 families, and 15 genera.
30498207	8	43	theme	microbiota	1324:1333	arg1	alteration					1335:1344	the microbiota alteration	1320:1344	the microbiota alteration	1320:1344	The degree of the microbiota alteration increased with the length of the postoperative time.
30498207	9	44	theme	PICRUSt	1403:1409	arg1	analysis					1411:1418	The PICRUSt analysis	1399:1418	The PICRUSt analysis	1399:1418	The PICRUSt analysis showed that the relative abundances of lipopolysaccharide biosynthesis proteins and lipopolysaccharide biosynthesis pathways were higher in the SP group and were positively associated with the plasma lipopolysaccharide level.
30498207	5	45	theme	Fecal	802:806	arg1	samples					808:814	Fecal samples	802:814	Fecal samples	802:814	Fecal samples were used for gut microbiota analysis via 16s rRNA gene sequencing, blood examinations and plasma lipopolysaccharide measurements were also taken.
30498207	6	46	theme	genera	1111:1116	arg1	abundances					1069:1078	the relative bacterial abundances	1046:1078	the relative bacterial abundances of 2 phyla, 7 families, and 15 genera	1046:1116	Significant differences were observed in gut microbiota composition with regard to the relative bacterial abundances of 2 phyla, 7 families, and 15 genera.
30498207	9	47	theme	proteins	1491:1498	arg1	abundances					1445:1454	the relative abundances	1432:1454	the relative abundances of lipopolysaccharide biosynthesis proteins and lipopolysaccharide biosynthesis pathways	1432:1543	The PICRUSt analysis showed that the relative abundances of lipopolysaccharide biosynthesis proteins and lipopolysaccharide biosynthesis pathways were higher in the SP group and were positively associated with the plasma lipopolysaccharide level.
30498207	9	47	theme	proteins	1491:1498	arg1	higher					1550:1555	higher	1550:1555	higher	1550:1555	The PICRUSt analysis showed that the relative abundances of lipopolysaccharide biosynthesis proteins and lipopolysaccharide biosynthesis pathways were higher in the SP group and were positively associated with the plasma lipopolysaccharide level.
30498207	7	48	theme	low	1275:1277	arg1	abundance					1279:1287	low abundance	1275:1287	low abundance in the SP group	1275:1303	The lipopolysaccharide level was significantly higher in the SP group than in the HC group and were negatively associated with five bacterial families with low abundance in the SP group.
30498207	0	49	theme	traumatic	29:37	arg1	splenectomy					39:49	traumatic splenectomy	29:49	traumatic splenectomy	29:49	Altered gut microbiota after traumatic splenectomy is associated with endotoxemia.
30498207	10	50	theme	Significant	1646:1656	arg1	alterations					1658:1668	Significant alterations	1646:1668	Significant alterations	1646:1668	Significant alterations were observed in the gut microbiota of the splenectomized patients and were associated with plasma lipopolysaccharide level.
30498207	9	51	theme	lipopolysaccharide	1459:1476	arg1	proteins					1491:1498	lipopolysaccharide biosynthesis proteins	1459:1498	lipopolysaccharide biosynthesis proteins	1459:1498	The PICRUSt analysis showed that the relative abundances of lipopolysaccharide biosynthesis proteins and lipopolysaccharide biosynthesis pathways were higher in the SP group and were positively associated with the plasma lipopolysaccharide level.
30498207	4	52	theme	time	796:799	arg1	length					766:771	the length	762:771	the length of their postoperative time	762:799	The SP group was subdivided into three subgroups according to the length of their postoperative time.
30498207	2	53	from	patients	371:378	arg1	cause					316:320	the potential cause	302:320	the potential cause of the high rate of infection in postsplenectomy patients	302:378	In this study, to increase our understanding of the potential cause of the high rate of infection in postsplenectomy patients, we evaluated the differences in the gut microbiota and plasma lipopolysaccharide level of patients after splenectomy relative to those of healthy controls.
30498207	5	54	theme	gut	830:832	arg1	analysis					845:852	gut microbiota analysis	830:852	gut microbiota analysis	830:852	Fecal samples were used for gut microbiota analysis via 16s rRNA gene sequencing, blood examinations and plasma lipopolysaccharide measurements were also taken.
30498207	2	55	theme	lipopolysaccharide	443:460	arg1	level					462:466	the gut microbiota and plasma lipopolysaccharide level	413:466	level	462:466	In this study, to increase our understanding of the potential cause of the high rate of infection in postsplenectomy patients, we evaluated the differences in the gut microbiota and plasma lipopolysaccharide level of patients after splenectomy relative to those of healthy controls.
30498207	10	56	theme	plasma	1762:1767	arg1	level					1788:1792	plasma lipopolysaccharide level	1762:1792	plasma lipopolysaccharide level	1762:1792	Significant alterations were observed in the gut microbiota of the splenectomized patients and were associated with plasma lipopolysaccharide level.
30498207	8	57	theme	postoperative	1379:1391	arg1	time					1393:1396	the postoperative time	1375:1396	the postoperative time	1375:1396	The degree of the microbiota alteration increased with the length of the postoperative time.
30498207	7	58	from	abundance	1279:1287	arg1	group					1299:1303	the SP group	1292:1303	the SP group	1292:1303	The lipopolysaccharide level was significantly higher in the SP group than in the HC group and were negatively associated with five bacterial families with low abundance in the SP group.
30498207	2	59	theme	high	329:332	arg1	rate					334:337	the high rate	325:337	the high rate of infection in postsplenectomy patients	325:378	In this study, to increase our understanding of the potential cause of the high rate of infection in postsplenectomy patients, we evaluated the differences in the gut microbiota and plasma lipopolysaccharide level of patients after splenectomy relative to those of healthy controls.
30498207	2	60	theme	plasma	436:441	arg1	level					462:466	the gut microbiota and plasma lipopolysaccharide level	413:466	level	462:466	In this study, to increase our understanding of the potential cause of the high rate of infection in postsplenectomy patients, we evaluated the differences in the gut microbiota and plasma lipopolysaccharide level of patients after splenectomy relative to those of healthy controls.
30498207	3	61	theme	healthy	593:599	arg1	individuals					601:611	42 healthy individuals	590:611	42 healthy individuals	590:611	Thirty-two patients having undergone splenectomy and 42 healthy individuals were enrolled into the splenectomy (SP) and healthy control (HC) groups, respectively.
30498207	1	62	theme	postoperative	123:135	arg1	infection					137:145	postoperative infection	123:145	postoperative infection	123:145	Splenectomy carries a long-term risk of postoperative infection, and the chronic, low-grade inflammation associated with endotoxemia may be related to the gut microbiota.
30498207	1	63	contain	carries	95:101	arg2	risk					115:118	a long-term risk	103:118	a long-term risk of postoperative infection	103:145	Splenectomy carries a long-term risk of postoperative infection, and the chronic, low-grade inflammation associated with endotoxemia may be related to the gut microbiota.
30498207	1	63	contain	carries	95:101	arg1	Splenectomy					83:93	Splenectomy	83:93	Splenectomy	83:93	Splenectomy carries a long-term risk of postoperative infection, and the chronic, low-grade inflammation associated with endotoxemia may be related to the gut microbiota.
30498207	9	64	theme	plasma	1613:1618	arg1	level					1639:1643	the plasma lipopolysaccharide level	1609:1643	the plasma lipopolysaccharide level	1609:1643	The PICRUSt analysis showed that the relative abundances of lipopolysaccharide biosynthesis proteins and lipopolysaccharide biosynthesis pathways were higher in the SP group and were positively associated with the plasma lipopolysaccharide level.
30498207	11	65	theme	such	1840:1843	arg1	alterations					1845:1855	such alterations	1840:1855	such alterations after splenectomy	1840:1873	Further studies are needed to verify whether such alterations after splenectomy are related to an increased risk of complications.
30498207	10	66	theme	splenectomized	1713:1726	arg1	patients					1728:1735	the splenectomized patients	1709:1735	the splenectomized patients	1709:1735	Significant alterations were observed in the gut microbiota of the splenectomized patients and were associated with plasma lipopolysaccharide level.
30498207	9	67	theme	pathways	1536:1543	arg1	abundances					1445:1454	the relative abundances	1432:1454	the relative abundances of lipopolysaccharide biosynthesis proteins and lipopolysaccharide biosynthesis pathways	1432:1543	The PICRUSt analysis showed that the relative abundances of lipopolysaccharide biosynthesis proteins and lipopolysaccharide biosynthesis pathways were higher in the SP group and were positively associated with the plasma lipopolysaccharide level.
30498207	9	67	theme	pathways	1536:1543	arg1	higher					1550:1555	higher	1550:1555	higher	1550:1555	The PICRUSt analysis showed that the relative abundances of lipopolysaccharide biosynthesis proteins and lipopolysaccharide biosynthesis pathways were higher in the SP group and were positively associated with the plasma lipopolysaccharide level.
30498207	1	68	theme	infection	137:145	arg1	risk					115:118	a long-term risk	103:118	a long-term risk of postoperative infection	103:145	Splenectomy carries a long-term risk of postoperative infection, and the chronic, low-grade inflammation associated with endotoxemia may be related to the gut microbiota.
30498207	10	69	theme	lipopolysaccharide	1769:1786	arg1	level					1788:1792	plasma lipopolysaccharide level	1762:1792	plasma lipopolysaccharide level	1762:1792	Significant alterations were observed in the gut microbiota of the splenectomized patients and were associated with plasma lipopolysaccharide level.
30498207	11	70	theme	Further	1795:1801	arg1	studies					1803:1809	Further studies	1795:1809	Further studies	1795:1809	Further studies are needed to verify whether such alterations after splenectomy are related to an increased risk of complications.
30498207	3	71	theme	healthy	657:663	arg1	control					665:671	healthy control	657:671	healthy control (HC)	657:676	Thirty-two patients having undergone splenectomy and 42 healthy individuals were enrolled into the splenectomy (SP) and healthy control (HC) groups, respectively.
30498207	3	71	theme	healthy	657:663	arg1	HC					674:675	HC	674:675	HC	674:675	Thirty-two patients having undergone splenectomy and 42 healthy individuals were enrolled into the splenectomy (SP) and healthy control (HC) groups, respectively.
30498207	3	72	theme	splenectomy	636:646	arg1	groups					678:683	the splenectomy (SP) and healthy control (HC) groups	632:683	the splenectomy (SP) and healthy control (HC) groups	632:683	Thirty-two patients having undergone splenectomy and 42 healthy individuals were enrolled into the splenectomy (SP) and healthy control (HC) groups, respectively.
30498207	10	73	theme	gut	1691:1693	arg1	microbiota					1695:1704	the gut microbiota	1687:1704	the gut microbiota of the splenectomized patients	1687:1735	Significant alterations were observed in the gut microbiota of the splenectomized patients and were associated with plasma lipopolysaccharide level.
30498207	2	74	theme	cause	316:320	arg1	understanding					285:297	our understanding	281:297	our understanding of the potential cause of the high rate of infection in postsplenectomy patients	281:378	In this study, to increase our understanding of the potential cause of the high rate of infection in postsplenectomy patients, we evaluated the differences in the gut microbiota and plasma lipopolysaccharide level of patients after splenectomy relative to those of healthy controls.
30498207	10	75	theme	patients	1728:1735	arg1	microbiota					1695:1704	the gut microbiota	1687:1704	the gut microbiota of the splenectomized patients	1687:1735	Significant alterations were observed in the gut microbiota of the splenectomized patients and were associated with plasma lipopolysaccharide level.
30498207	2	76	theme	relative	498:505	arg1	splenectomy					486:496	splenectomy	486:496	splenectomy relative to those of healthy controls	486:534	In this study, to increase our understanding of the potential cause of the high rate of infection in postsplenectomy patients, we evaluated the differences in the gut microbiota and plasma lipopolysaccharide level of patients after splenectomy relative to those of healthy controls.
30498207	3	77	theme	control	665:671	arg1	groups					678:683	the splenectomy (SP) and healthy control (HC) groups	632:683	the splenectomy (SP) and healthy control (HC) groups	632:683	Thirty-two patients having undergone splenectomy and 42 healthy individuals were enrolled into the splenectomy (SP) and healthy control (HC) groups, respectively.
30498207	4	78	theme	postoperative	782:794	arg1	time					796:799	their postoperative time	776:799	their postoperative time	776:799	The SP group was subdivided into three subgroups according to the length of their postoperative time.
30498207	9	79	theme	biosynthesis	1523:1534	arg1	pathways					1536:1543	lipopolysaccharide biosynthesis pathways	1504:1543	lipopolysaccharide biosynthesis pathways	1504:1543	The PICRUSt analysis showed that the relative abundances of lipopolysaccharide biosynthesis proteins and lipopolysaccharide biosynthesis pathways were higher in the SP group and were positively associated with the plasma lipopolysaccharide level.
30498207	5	80	theme	rRNA	862:865	arg1	sequencing					872:881	rRNA gene sequencing	862:881	rRNA gene sequencing	862:881	Fecal samples were used for gut microbiota analysis via 16s rRNA gene sequencing, blood examinations and plasma lipopolysaccharide measurements were also taken.
30498207	1	81	theme	gut	238:240	arg1	microbiota					242:251	the gut microbiota	234:251	the gut microbiota	234:251	Splenectomy carries a long-term risk of postoperative infection, and the chronic, low-grade inflammation associated with endotoxemia may be related to the gut microbiota.
30498207	2	82	theme	infection	342:350	arg1	rate					334:337	the high rate	325:337	the high rate of infection in postsplenectomy patients	325:378	In this study, to increase our understanding of the potential cause of the high rate of infection in postsplenectomy patients, we evaluated the differences in the gut microbiota and plasma lipopolysaccharide level of patients after splenectomy relative to those of healthy controls.
30498207	9	83	theme	lipopolysaccharide	1620:1637	arg1	level					1639:1643	the plasma lipopolysaccharide level	1609:1643	the plasma lipopolysaccharide level	1609:1643	The PICRUSt analysis showed that the relative abundances of lipopolysaccharide biosynthesis proteins and lipopolysaccharide biosynthesis pathways were higher in the SP group and were positively associated with the plasma lipopolysaccharide level.
30498207	6	84	theme	microbiota	1008:1017	arg1	composition					1019:1029	gut microbiota composition	1004:1029	gut microbiota composition	1004:1029	Significant differences were observed in gut microbiota composition with regard to the relative bacterial abundances of 2 phyla, 7 families, and 15 genera.
30497605	2	0	theme	deposition	466:475	arg1	rate					477:480	the deposition rate	462:480	the deposition rate of lees	462:488	This research aimed to: develop yeast immobilisation processes on two wine-compatible supports; study the effects of yeast type (IOC 18-2007 and 55A) and the immobilisation support type (oak chips and cellulose powder) on the fermentation kinetics, the deposition rate of lees and the volatile composition of the finished sparkling wine; compare the fermentation parameters of the wines inoculated with immobilised or non-immobilised cells.
30497605	2	0	theme	deposition	466:475	arg1	kinetics					452:459	the fermentation kinetics	435:459	the fermentation kinetics	435:459	This research aimed to: develop yeast immobilisation processes on two wine-compatible supports; study the effects of yeast type (IOC 18-2007 and 55A) and the immobilisation support type (oak chips and cellulose powder) on the fermentation kinetics, the deposition rate of lees and the volatile composition of the finished sparkling wine; compare the fermentation parameters of the wines inoculated with immobilised or non-immobilised cells.
30497605	3	1	from	immobilisation	661:674	arg1	chips					692:696	oak chips	688:696	oak chips	688:696	Proper immobilisation of yeast on oak chips and cellulose powder was demonstrated by electron microscopy.
30497605	3	1	from	immobilisation	661:674	arg1	powder					712:717	cellulose powder	702:717	cellulose powder	702:717	Proper immobilisation of yeast on oak chips and cellulose powder was demonstrated by electron microscopy.
30497605	7	2	theme	yeast	1328:1332	arg1	immobilisation					1334:1347	yeast immobilisation	1328:1347	yeast immobilisation	1328:1347	Oak chips were the more appropriate support for yeast immobilisation.
30497605	6	3	theme	wines	1115:1119	arg1	compounds					1079:1087	the volatile compounds	1066:1087	the volatile compounds of the finished sparkling wines	1066:1119	The analysis of the volatile compounds of the finished sparkling wines showed significant differences in the formation of esters, acids, alcohols, aldehydes and lactones according to the yeast and the immobilisation support used.
30497605	4	4	dep	way	864:866	arg1	they					868:871	they	868:871	they	868:871	Total sugar consumption occurred in under 60 days in all bottles, regardless of the strain used and the way they were inoculated in wine.
30497605	3	5	theme	yeast	679:683	arg1	immobilisation					661:674	Proper immobilisation	654:674	Proper immobilisation of yeast on oak chips and cellulose powder	654:717	Proper immobilisation of yeast on oak chips and cellulose powder was demonstrated by electron microscopy.
30497605	6	6	theme	aldehydes	1197:1205	arg1	formation					1159:1167	the formation	1155:1167	the formation of esters, acids, alcohols, aldehydes and lactones	1155:1218	The analysis of the volatile compounds of the finished sparkling wines showed significant differences in the formation of esters, acids, alcohols, aldehydes and lactones according to the yeast and the immobilisation support used.
30497605	0	7	theme	sparkling	86:94	arg1	wine					96:99	bottle-fermented sparkling wine	69:99	bottle-fermented sparkling wine	69:99	Immobilisation of yeasts on oak chips or cellulose powder for use in bottle-fermented sparkling wine.
30497605	0	8	from	Immobilisation	0:13	arg1	chips					32:36	oak chips	28:36	oak chips	28:36	Immobilisation of yeasts on oak chips or cellulose powder for use in bottle-fermented sparkling wine.
30497605	0	8	from	Immobilisation	0:13	arg1	powder					51:56	cellulose powder	41:56	cellulose powder	41:56	Immobilisation of yeasts on oak chips or cellulose powder for use in bottle-fermented sparkling wine.
30497605	2	9	theme	fermentation	563:574	arg1	parameters					576:585	the fermentation parameters	559:585	the fermentation parameters of the wines inoculated with immobilised or non-immobilised cells	559:651	This research aimed to: develop yeast immobilisation processes on two wine-compatible supports; study the effects of yeast type (IOC 18-2007 and 55A) and the immobilisation support type (oak chips and cellulose powder) on the fermentation kinetics, the deposition rate of lees and the volatile composition of the finished sparkling wine; compare the fermentation parameters of the wines inoculated with immobilised or non-immobilised cells.
30497605	2	10	theme	non-immobilised	631:645	arg1	cells					647:651	immobilised or non-immobilised cells	616:651	immobilised or non-immobilised cells	616:651	This research aimed to: develop yeast immobilisation processes on two wine-compatible supports; study the effects of yeast type (IOC 18-2007 and 55A) and the immobilisation support type (oak chips and cellulose powder) on the fermentation kinetics, the deposition rate of lees and the volatile composition of the finished sparkling wine; compare the fermentation parameters of the wines inoculated with immobilised or non-immobilised cells.
30497605	4	11	theme	Total	760:764	arg1	consumption					772:782	Total sugar consumption	760:782	Total sugar consumption	760:782	Total sugar consumption occurred in under 60 days in all bottles, regardless of the strain used and the way they were inoculated in wine.
30497605	5	12	contain	containing	950:959	arg2	cells					973:977	immobilised cells	961:977	immobilised cells	961:977	Deposition of lees was 3-fold faster in the bottles containing immobilised cells than in those with free cells; no addition of adjuvants was necessary.
30497605	5	12	contain	containing	950:959	arg1	bottles					942:948	the bottles	938:948	the bottles containing immobilised cells	938:977	Deposition of lees was 3-fold faster in the bottles containing immobilised cells than in those with free cells; no addition of adjuvants was necessary.
30497605	0	13	theme	bottle-fermented	69:84	arg1	wine					96:99	bottle-fermented sparkling wine	69:99	bottle-fermented sparkling wine	69:99	Immobilisation of yeasts on oak chips or cellulose powder for use in bottle-fermented sparkling wine.
30497605	8	14	from	differences	1365:1375	arg1	analysis					1394:1401	the sensorial analysis	1380:1401	the sensorial analysis of the sparkling wines produced by the different strategies	1380:1461	No significant differences in the sensorial analysis of the sparkling wines produced by the different strategies were found.
30497605	2	15	dep	effects	319:325	arg1	study					309:313	study	309:313	study	309:313	This research aimed to: develop yeast immobilisation processes on two wine-compatible supports; study the effects of yeast type (IOC 18-2007 and 55A) and the immobilisation support type (oak chips and cellulose powder) on the fermentation kinetics, the deposition rate of lees and the volatile composition of the finished sparkling wine; compare the fermentation parameters of the wines inoculated with immobilised or non-immobilised cells.
30497605	6	16	theme	lactones	1211:1218	arg1	formation					1159:1167	the formation	1155:1167	the formation of esters, acids, alcohols, aldehydes and lactones	1155:1218	The analysis of the volatile compounds of the finished sparkling wines showed significant differences in the formation of esters, acids, alcohols, aldehydes and lactones according to the yeast and the immobilisation support used.
30497605	2	17	theme	immobilised	616:626	arg1	cells					647:651	immobilised or non-immobilised cells	616:651	immobilised or non-immobilised cells	616:651	This research aimed to: develop yeast immobilisation processes on two wine-compatible supports; study the effects of yeast type (IOC 18-2007 and 55A) and the immobilisation support type (oak chips and cellulose powder) on the fermentation kinetics, the deposition rate of lees and the volatile composition of the finished sparkling wine; compare the fermentation parameters of the wines inoculated with immobilised or non-immobilised cells.
30497605	6	18	theme	significant	1128:1138	arg1	differences					1140:1150	significant differences	1128:1150	significant differences in the formation of esters, acids, alcohols, aldehydes and lactones	1128:1218	The analysis of the volatile compounds of the finished sparkling wines showed significant differences in the formation of esters, acids, alcohols, aldehydes and lactones according to the yeast and the immobilisation support used.
30497605	2	19	theme	immobilisation	371:384	arg1	type					394:397	the immobilisation support type	367:397	the immobilisation support type (oak chips and cellulose powder)	367:430	This research aimed to: develop yeast immobilisation processes on two wine-compatible supports; study the effects of yeast type (IOC 18-2007 and 55A) and the immobilisation support type (oak chips and cellulose powder) on the fermentation kinetics, the deposition rate of lees and the volatile composition of the finished sparkling wine; compare the fermentation parameters of the wines inoculated with immobilised or non-immobilised cells.
30497605	2	20	theme	type	394:397	arg1	effects					319:325	the effects	315:325	the effects of yeast type (IOC 18-2007 and 55A) and the immobilisation support type (oak chips and cellulose powder) on the fermentation kinetics, the deposition rate of lees and the volatile composition of the finished sparkling wine	315:548	This research aimed to: develop yeast immobilisation processes on two wine-compatible supports; study the effects of yeast type (IOC 18-2007 and 55A) and the immobilisation support type (oak chips and cellulose powder) on the fermentation kinetics, the deposition rate of lees and the volatile composition of the finished sparkling wine; compare the fermentation parameters of the wines inoculated with immobilised or non-immobilised cells.
30497605	2	21	theme	lees	485:488	arg1	rate					477:480	the deposition rate	462:480	the deposition rate of lees	462:488	This research aimed to: develop yeast immobilisation processes on two wine-compatible supports; study the effects of yeast type (IOC 18-2007 and 55A) and the immobilisation support type (oak chips and cellulose powder) on the fermentation kinetics, the deposition rate of lees and the volatile composition of the finished sparkling wine; compare the fermentation parameters of the wines inoculated with immobilised or non-immobilised cells.
30497605	2	21	theme	lees	485:488	arg1	composition					507:517	the volatile composition	494:517	the volatile composition of the finished sparkling wine	494:548	This research aimed to: develop yeast immobilisation processes on two wine-compatible supports; study the effects of yeast type (IOC 18-2007 and 55A) and the immobilisation support type (oak chips and cellulose powder) on the fermentation kinetics, the deposition rate of lees and the volatile composition of the finished sparkling wine; compare the fermentation parameters of the wines inoculated with immobilised or non-immobilised cells.
30497605	2	21	theme	lees	485:488	arg1	kinetics					452:459	the fermentation kinetics	435:459	the fermentation kinetics	435:459	This research aimed to: develop yeast immobilisation processes on two wine-compatible supports; study the effects of yeast type (IOC 18-2007 and 55A) and the immobilisation support type (oak chips and cellulose powder) on the fermentation kinetics, the deposition rate of lees and the volatile composition of the finished sparkling wine; compare the fermentation parameters of the wines inoculated with immobilised or non-immobilised cells.
30497605	4	22	theme	sugar	766:770	arg1	consumption					772:782	Total sugar consumption	760:782	Total sugar consumption	760:782	Total sugar consumption occurred in under 60 days in all bottles, regardless of the strain used and the way they were inoculated in wine.
30497605	2	23	theme	support	386:392	arg1	type					394:397	the immobilisation support type	367:397	the immobilisation support type (oak chips and cellulose powder)	367:430	This research aimed to: develop yeast immobilisation processes on two wine-compatible supports; study the effects of yeast type (IOC 18-2007 and 55A) and the immobilisation support type (oak chips and cellulose powder) on the fermentation kinetics, the deposition rate of lees and the volatile composition of the finished sparkling wine; compare the fermentation parameters of the wines inoculated with immobilised or non-immobilised cells.
30497605	7	24	theme	appropriate	1304:1314	arg1	support					1316:1322	the more appropriate support	1295:1322	the more appropriate support for yeast immobilisation	1295:1347	Oak chips were the more appropriate support for yeast immobilisation.
30497605	7	24	theme	appropriate	1304:1314	arg1	chips					1284:1288	Oak chips	1280:1288	Oak chips	1280:1288	Oak chips were the more appropriate support for yeast immobilisation.
30497605	3	25	theme	cellulose	702:710	arg1	powder					712:717	cellulose powder	702:717	cellulose powder	702:717	Proper immobilisation of yeast on oak chips and cellulose powder was demonstrated by electron microscopy.
30497605	2	26	theme	wines	594:598	arg1	parameters					576:585	the fermentation parameters	559:585	the fermentation parameters of the wines inoculated with immobilised or non-immobilised cells	559:651	This research aimed to: develop yeast immobilisation processes on two wine-compatible supports; study the effects of yeast type (IOC 18-2007 and 55A) and the immobilisation support type (oak chips and cellulose powder) on the fermentation kinetics, the deposition rate of lees and the volatile composition of the finished sparkling wine; compare the fermentation parameters of the wines inoculated with immobilised or non-immobilised cells.
30497605	2	27	theme	yeast	330:334	arg1	type					336:339	yeast type	330:339	yeast type (IOC 18-2007 and 55A)	330:361	This research aimed to: develop yeast immobilisation processes on two wine-compatible supports; study the effects of yeast type (IOC 18-2007 and 55A) and the immobilisation support type (oak chips and cellulose powder) on the fermentation kinetics, the deposition rate of lees and the volatile composition of the finished sparkling wine; compare the fermentation parameters of the wines inoculated with immobilised or non-immobilised cells.
30497605	6	28	from	differences	1140:1150	arg1	formation					1159:1167	the formation	1155:1167	the formation of esters, acids, alcohols, aldehydes and lactones	1155:1218	The analysis of the volatile compounds of the finished sparkling wines showed significant differences in the formation of esters, acids, alcohols, aldehydes and lactones according to the yeast and the immobilisation support used.
30497605	1	29	theme	cerevisiae	193:202	arg1	strains					204:210	Sacharomyces cerevisiae strains	180:210	Sacharomyces cerevisiae strains	180:210	Sparkling wine production comprises two successive fermentations performed by Sacharomyces cerevisiae strains.
30497605	3	30	theme	electron	739:746	arg1	microscopy					748:757	electron microscopy	739:757	electron microscopy	739:757	Proper immobilisation of yeast on oak chips and cellulose powder was demonstrated by electron microscopy.
30497605	2	31	dep	type	336:339	arg1	IOC					342:344	IOC 18-2007	342:352	IOC 18-2007	342:352	This research aimed to: develop yeast immobilisation processes on two wine-compatible supports; study the effects of yeast type (IOC 18-2007 and 55A) and the immobilisation support type (oak chips and cellulose powder) on the fermentation kinetics, the deposition rate of lees and the volatile composition of the finished sparkling wine; compare the fermentation parameters of the wines inoculated with immobilised or non-immobilised cells.
30497605	2	31	dep	type	336:339	arg1	55A					358:360	55A	358:360	55A	358:360	This research aimed to: develop yeast immobilisation processes on two wine-compatible supports; study the effects of yeast type (IOC 18-2007 and 55A) and the immobilisation support type (oak chips and cellulose powder) on the fermentation kinetics, the deposition rate of lees and the volatile composition of the finished sparkling wine; compare the fermentation parameters of the wines inoculated with immobilised or non-immobilised cells.
30497605	2	32	from	processes	266:274	arg1	wine-compatible					283:297	wine-compatible	283:297	wine-compatible	283:297	This research aimed to: develop yeast immobilisation processes on two wine-compatible supports; study the effects of yeast type (IOC 18-2007 and 55A) and the immobilisation support type (oak chips and cellulose powder) on the fermentation kinetics, the deposition rate of lees and the volatile composition of the finished sparkling wine; compare the fermentation parameters of the wines inoculated with immobilised or non-immobilised cells.
30497605	5	33	theme	adjuvants	1025:1033	arg1	addition					1013:1020	no addition	1010:1020	no addition of adjuvants	1010:1033	Deposition of lees was 3-fold faster in the bottles containing immobilised cells than in those with free cells; no addition of adjuvants was necessary.
30497605	6	34	dep	support	1266:1272	arg1	used					1274:1277	used	1274:1277	support used	1266:1277	The analysis of the volatile compounds of the finished sparkling wines showed significant differences in the formation of esters, acids, alcohols, aldehydes and lactones according to the yeast and the immobilisation support used.
30497605	2	35	theme	finished	526:533	arg1	wine					545:548	the finished sparkling wine	522:548	the finished sparkling wine	522:548	This research aimed to: develop yeast immobilisation processes on two wine-compatible supports; study the effects of yeast type (IOC 18-2007 and 55A) and the immobilisation support type (oak chips and cellulose powder) on the fermentation kinetics, the deposition rate of lees and the volatile composition of the finished sparkling wine; compare the fermentation parameters of the wines inoculated with immobilised or non-immobilised cells.
30497605	8	36	theme	significant	1353:1363	arg1	differences					1365:1375	No significant differences	1350:1375	No significant differences in the sensorial analysis of the sparkling wines produced by the different strategies	1350:1461	No significant differences in the sensorial analysis of the sparkling wines produced by the different strategies were found.
30497605	6	37	theme	finished	1096:1103	arg1	wines					1115:1119	the finished sparkling wines	1092:1119	the finished sparkling wines	1092:1119	The analysis of the volatile compounds of the finished sparkling wines showed significant differences in the formation of esters, acids, alcohols, aldehydes and lactones according to the yeast and the immobilisation support used.
30497605	6	38	theme	alcohols	1187:1194	arg1	formation					1159:1167	the formation	1155:1167	the formation of esters, acids, alcohols, aldehydes and lactones	1155:1218	The analysis of the volatile compounds of the finished sparkling wines showed significant differences in the formation of esters, acids, alcohols, aldehydes and lactones according to the yeast and the immobilisation support used.
30497605	0	39	theme	yeasts	18:23	arg1	Immobilisation					0:13	Immobilisation	0:13	Immobilisation of yeasts on oak chips or cellulose powder for use in bottle-fermented sparkling wine.	0:100	Immobilisation of yeasts on oak chips or cellulose powder for use in bottle-fermented sparkling wine.
30497605	5	40	theme	lees	912:915	arg1	Deposition					898:907	Deposition	898:907	Deposition of lees	898:915	Deposition of lees was 3-fold faster in the bottles containing immobilised cells than in those with free cells; no addition of adjuvants was necessary.
30497605	6	41	theme	sparkling	1105:1113	arg1	wines					1115:1119	the finished sparkling wines	1092:1119	the finished sparkling wines	1092:1119	The analysis of the volatile compounds of the finished sparkling wines showed significant differences in the formation of esters, acids, alcohols, aldehydes and lactones according to the yeast and the immobilisation support used.
30497605	6	42	theme	compounds	1079:1087	arg1	analysis					1054:1061	The analysis	1050:1061	The analysis of the volatile compounds of the finished sparkling wines	1050:1119	The analysis of the volatile compounds of the finished sparkling wines showed significant differences in the formation of esters, acids, alcohols, aldehydes and lactones according to the yeast and the immobilisation support used.
30497605	0	43	from	use	62:64	arg1	wine					96:99	bottle-fermented sparkling wine	69:99	bottle-fermented sparkling wine	69:99	Immobilisation of yeasts on oak chips or cellulose powder for use in bottle-fermented sparkling wine.
30497605	2	44	theme	yeast	245:249	arg1	processes					266:274	yeast immobilisation processes	245:274	yeast immobilisation processes on two wine-compatible supports	245:306	This research aimed to: develop yeast immobilisation processes on two wine-compatible supports; study the effects of yeast type (IOC 18-2007 and 55A) and the immobilisation support type (oak chips and cellulose powder) on the fermentation kinetics, the deposition rate of lees and the volatile composition of the finished sparkling wine; compare the fermentation parameters of the wines inoculated with immobilised or non-immobilised cells.
30497605	2	45	theme	oak	400:402	arg1	chips					404:408	oak chips	400:408	oak chips	400:408	This research aimed to: develop yeast immobilisation processes on two wine-compatible supports; study the effects of yeast type (IOC 18-2007 and 55A) and the immobilisation support type (oak chips and cellulose powder) on the fermentation kinetics, the deposition rate of lees and the volatile composition of the finished sparkling wine; compare the fermentation parameters of the wines inoculated with immobilised or non-immobilised cells.
30497605	0	46	theme	oak	28:30	arg1	chips					32:36	oak chips	28:36	oak chips	28:36	Immobilisation of yeasts on oak chips or cellulose powder for use in bottle-fermented sparkling wine.
30497605	7	47	theme	Oak	1280:1282	arg1	support					1316:1322	the more appropriate support	1295:1322	the more appropriate support for yeast immobilisation	1295:1347	Oak chips were the more appropriate support for yeast immobilisation.
30497605	7	47	theme	Oak	1280:1282	arg1	chips					1284:1288	Oak chips	1280:1288	Oak chips	1280:1288	Oak chips were the more appropriate support for yeast immobilisation.
30497605	0	48	theme	cellulose	41:49	arg1	powder					51:56	cellulose powder	41:56	cellulose powder	41:56	Immobilisation of yeasts on oak chips or cellulose powder for use in bottle-fermented sparkling wine.
30497605	2	49	theme	volatile	498:505	arg1	composition					507:517	the volatile composition	494:517	the volatile composition of the finished sparkling wine	494:548	This research aimed to: develop yeast immobilisation processes on two wine-compatible supports; study the effects of yeast type (IOC 18-2007 and 55A) and the immobilisation support type (oak chips and cellulose powder) on the fermentation kinetics, the deposition rate of lees and the volatile composition of the finished sparkling wine; compare the fermentation parameters of the wines inoculated with immobilised or non-immobilised cells.
30497605	2	49	theme	volatile	498:505	arg1	kinetics					452:459	the fermentation kinetics	435:459	the fermentation kinetics	435:459	This research aimed to: develop yeast immobilisation processes on two wine-compatible supports; study the effects of yeast type (IOC 18-2007 and 55A) and the immobilisation support type (oak chips and cellulose powder) on the fermentation kinetics, the deposition rate of lees and the volatile composition of the finished sparkling wine; compare the fermentation parameters of the wines inoculated with immobilised or non-immobilised cells.
30497605	8	50	theme	sparkling	1410:1418	arg1	wines					1420:1424	the sparkling wines	1406:1424	the sparkling wines produced by the different strategies	1406:1461	No significant differences in the sensorial analysis of the sparkling wines produced by the different strategies were found.
30497605	6	51	theme	acids	1180:1184	arg1	formation					1159:1167	the formation	1155:1167	the formation of esters, acids, alcohols, aldehydes and lactones	1155:1218	The analysis of the volatile compounds of the finished sparkling wines showed significant differences in the formation of esters, acids, alcohols, aldehydes and lactones according to the yeast and the immobilisation support used.
30497605	1	52	theme	Sparkling	102:110	arg1	production					117:126	Sparkling wine production	102:126	Sparkling wine production	102:126	Sparkling wine production comprises two successive fermentations performed by Sacharomyces cerevisiae strains.
30497605	5	53	theme	free	998:1001	arg1	cells					1003:1007	free cells	998:1007	free cells	998:1007	Deposition of lees was 3-fold faster in the bottles containing immobilised cells than in those with free cells; no addition of adjuvants was necessary.
30497605	1	54	theme	wine	112:115	arg1	production					117:126	Sparkling wine production	102:126	Sparkling wine production	102:126	Sparkling wine production comprises two successive fermentations performed by Sacharomyces cerevisiae strains.
30497605	8	55	theme	sensorial	1384:1392	arg1	analysis					1394:1401	the sensorial analysis	1380:1401	the sensorial analysis of the sparkling wines produced by the different strategies	1380:1461	No significant differences in the sensorial analysis of the sparkling wines produced by the different strategies were found.
30497605	2	56	theme	wine	545:548	arg1	rate					477:480	the deposition rate	462:480	the deposition rate of lees	462:488	This research aimed to: develop yeast immobilisation processes on two wine-compatible supports; study the effects of yeast type (IOC 18-2007 and 55A) and the immobilisation support type (oak chips and cellulose powder) on the fermentation kinetics, the deposition rate of lees and the volatile composition of the finished sparkling wine; compare the fermentation parameters of the wines inoculated with immobilised or non-immobilised cells.
30497605	2	56	theme	wine	545:548	arg1	composition					507:517	the volatile composition	494:517	the volatile composition of the finished sparkling wine	494:548	This research aimed to: develop yeast immobilisation processes on two wine-compatible supports; study the effects of yeast type (IOC 18-2007 and 55A) and the immobilisation support type (oak chips and cellulose powder) on the fermentation kinetics, the deposition rate of lees and the volatile composition of the finished sparkling wine; compare the fermentation parameters of the wines inoculated with immobilised or non-immobilised cells.
30497605	2	56	theme	wine	545:548	arg1	kinetics					452:459	the fermentation kinetics	435:459	the fermentation kinetics	435:459	This research aimed to: develop yeast immobilisation processes on two wine-compatible supports; study the effects of yeast type (IOC 18-2007 and 55A) and the immobilisation support type (oak chips and cellulose powder) on the fermentation kinetics, the deposition rate of lees and the volatile composition of the finished sparkling wine; compare the fermentation parameters of the wines inoculated with immobilised or non-immobilised cells.
30497605	6	57	theme	esters	1172:1177	arg1	formation					1159:1167	the formation	1155:1167	the formation of esters, acids, alcohols, aldehydes and lactones	1155:1218	The analysis of the volatile compounds of the finished sparkling wines showed significant differences in the formation of esters, acids, alcohols, aldehydes and lactones according to the yeast and the immobilisation support used.
30497605	6	58	theme	volatile	1070:1077	arg1	compounds					1079:1087	the volatile compounds	1066:1087	the volatile compounds of the finished sparkling wines	1066:1119	The analysis of the volatile compounds of the finished sparkling wines showed significant differences in the formation of esters, acids, alcohols, aldehydes and lactones according to the yeast and the immobilisation support used.
30497605	2	59	theme	sparkling	535:543	arg1	wine					545:548	the finished sparkling wine	522:548	the finished sparkling wine	522:548	This research aimed to: develop yeast immobilisation processes on two wine-compatible supports; study the effects of yeast type (IOC 18-2007 and 55A) and the immobilisation support type (oak chips and cellulose powder) on the fermentation kinetics, the deposition rate of lees and the volatile composition of the finished sparkling wine; compare the fermentation parameters of the wines inoculated with immobilised or non-immobilised cells.
30497605	8	60	theme	different	1442:1450	arg1	strategies					1452:1461	the different strategies	1438:1461	the different strategies	1438:1461	No significant differences in the sensorial analysis of the sparkling wines produced by the different strategies were found.
30497605	5	61	theme	immobilised	961:971	arg1	cells					973:977	immobilised cells	961:977	immobilised cells	961:977	Deposition of lees was 3-fold faster in the bottles containing immobilised cells than in those with free cells; no addition of adjuvants was necessary.
30497605	2	62	dep	wine-compatible	283:297	arg1	supports					299:306	supports	299:306	supports	299:306	This research aimed to: develop yeast immobilisation processes on two wine-compatible supports; study the effects of yeast type (IOC 18-2007 and 55A) and the immobilisation support type (oak chips and cellulose powder) on the fermentation kinetics, the deposition rate of lees and the volatile composition of the finished sparkling wine; compare the fermentation parameters of the wines inoculated with immobilised or non-immobilised cells.
30497605	2	63	theme	type	336:339	arg1	effects					319:325	the effects	315:325	the effects of yeast type (IOC 18-2007 and 55A) and the immobilisation support type (oak chips and cellulose powder) on the fermentation kinetics, the deposition rate of lees and the volatile composition of the finished sparkling wine	315:548	This research aimed to: develop yeast immobilisation processes on two wine-compatible supports; study the effects of yeast type (IOC 18-2007 and 55A) and the immobilisation support type (oak chips and cellulose powder) on the fermentation kinetics, the deposition rate of lees and the volatile composition of the finished sparkling wine; compare the fermentation parameters of the wines inoculated with immobilised or non-immobilised cells.
30497605	6	64	dep	immobilisation	1251:1264	arg1	support					1266:1272	support	1266:1272	support used	1266:1277	The analysis of the volatile compounds of the finished sparkling wines showed significant differences in the formation of esters, acids, alcohols, aldehydes and lactones according to the yeast and the immobilisation support used.
30497605	8	65	theme	wines	1420:1424	arg1	analysis					1394:1401	the sensorial analysis	1380:1401	the sensorial analysis of the sparkling wines produced by the different strategies	1380:1461	No significant differences in the sensorial analysis of the sparkling wines produced by the different strategies were found.
30497605	3	66	theme	Proper	654:659	arg1	immobilisation					661:674	Proper immobilisation	654:674	Proper immobilisation of yeast on oak chips and cellulose powder	654:717	Proper immobilisation of yeast on oak chips and cellulose powder was demonstrated by electron microscopy.
30497605	2	67	dep	type	394:397	arg1	chips					404:408	oak chips	400:408	oak chips	400:408	This research aimed to: develop yeast immobilisation processes on two wine-compatible supports; study the effects of yeast type (IOC 18-2007 and 55A) and the immobilisation support type (oak chips and cellulose powder) on the fermentation kinetics, the deposition rate of lees and the volatile composition of the finished sparkling wine; compare the fermentation parameters of the wines inoculated with immobilised or non-immobilised cells.
30497605	2	67	dep	type	394:397	arg1	powder					424:429	cellulose powder	414:429	cellulose powder	414:429	This research aimed to: develop yeast immobilisation processes on two wine-compatible supports; study the effects of yeast type (IOC 18-2007 and 55A) and the immobilisation support type (oak chips and cellulose powder) on the fermentation kinetics, the deposition rate of lees and the volatile composition of the finished sparkling wine; compare the fermentation parameters of the wines inoculated with immobilised or non-immobilised cells.
30497605	2	68	theme	cellulose	414:422	arg1	powder					424:429	cellulose powder	414:429	cellulose powder	414:429	This research aimed to: develop yeast immobilisation processes on two wine-compatible supports; study the effects of yeast type (IOC 18-2007 and 55A) and the immobilisation support type (oak chips and cellulose powder) on the fermentation kinetics, the deposition rate of lees and the volatile composition of the finished sparkling wine; compare the fermentation parameters of the wines inoculated with immobilised or non-immobilised cells.
30497605	1	69	theme	Sacharomyces	180:191	arg1	strains					204:210	Sacharomyces cerevisiae strains	180:210	Sacharomyces cerevisiae strains	180:210	Sparkling wine production comprises two successive fermentations performed by Sacharomyces cerevisiae strains.
30497605	2	70	theme	fermentation	439:450	arg1	rate					477:480	the deposition rate	462:480	the deposition rate of lees	462:488	This research aimed to: develop yeast immobilisation processes on two wine-compatible supports; study the effects of yeast type (IOC 18-2007 and 55A) and the immobilisation support type (oak chips and cellulose powder) on the fermentation kinetics, the deposition rate of lees and the volatile composition of the finished sparkling wine; compare the fermentation parameters of the wines inoculated with immobilised or non-immobilised cells.
30497605	2	70	theme	fermentation	439:450	arg1	composition					507:517	the volatile composition	494:517	the volatile composition of the finished sparkling wine	494:548	This research aimed to: develop yeast immobilisation processes on two wine-compatible supports; study the effects of yeast type (IOC 18-2007 and 55A) and the immobilisation support type (oak chips and cellulose powder) on the fermentation kinetics, the deposition rate of lees and the volatile composition of the finished sparkling wine; compare the fermentation parameters of the wines inoculated with immobilised or non-immobilised cells.
30497605	2	70	theme	fermentation	439:450	arg1	kinetics					452:459	the fermentation kinetics	435:459	the fermentation kinetics	435:459	This research aimed to: develop yeast immobilisation processes on two wine-compatible supports; study the effects of yeast type (IOC 18-2007 and 55A) and the immobilisation support type (oak chips and cellulose powder) on the fermentation kinetics, the deposition rate of lees and the volatile composition of the finished sparkling wine; compare the fermentation parameters of the wines inoculated with immobilised or non-immobilised cells.
30497605	3	71	theme	oak	688:690	arg1	chips					692:696	oak chips	688:696	oak chips	688:696	Proper immobilisation of yeast on oak chips and cellulose powder was demonstrated by electron microscopy.
30497605	2	72	theme	immobilisation	251:264	arg1	processes					266:274	yeast immobilisation processes	245:274	yeast immobilisation processes on two wine-compatible supports	245:306	This research aimed to: develop yeast immobilisation processes on two wine-compatible supports; study the effects of yeast type (IOC 18-2007 and 55A) and the immobilisation support type (oak chips and cellulose powder) on the fermentation kinetics, the deposition rate of lees and the volatile composition of the finished sparkling wine; compare the fermentation parameters of the wines inoculated with immobilised or non-immobilised cells.
30497605	1	73	theme	successive	142:151	arg1	fermentations					153:165	two successive fermentations	138:165	two successive fermentations performed by Sacharomyces cerevisiae strains	138:210	Sparkling wine production comprises two successive fermentations performed by Sacharomyces cerevisiae strains.
30497605	2	74	from	effects	319:325	arg1	rate					477:480	the deposition rate	462:480	the deposition rate of lees	462:488	This research aimed to: develop yeast immobilisation processes on two wine-compatible supports; study the effects of yeast type (IOC 18-2007 and 55A) and the immobilisation support type (oak chips and cellulose powder) on the fermentation kinetics, the deposition rate of lees and the volatile composition of the finished sparkling wine; compare the fermentation parameters of the wines inoculated with immobilised or non-immobilised cells.
30497605	2	74	from	effects	319:325	arg1	composition					507:517	the volatile composition	494:517	the volatile composition of the finished sparkling wine	494:548	This research aimed to: develop yeast immobilisation processes on two wine-compatible supports; study the effects of yeast type (IOC 18-2007 and 55A) and the immobilisation support type (oak chips and cellulose powder) on the fermentation kinetics, the deposition rate of lees and the volatile composition of the finished sparkling wine; compare the fermentation parameters of the wines inoculated with immobilised or non-immobilised cells.
30497605	2	74	from	effects	319:325	arg1	kinetics					452:459	the fermentation kinetics	435:459	the fermentation kinetics	435:459	This research aimed to: develop yeast immobilisation processes on two wine-compatible supports; study the effects of yeast type (IOC 18-2007 and 55A) and the immobilisation support type (oak chips and cellulose powder) on the fermentation kinetics, the deposition rate of lees and the volatile composition of the finished sparkling wine; compare the fermentation parameters of the wines inoculated with immobilised or non-immobilised cells.
30234478	8	0	from	peptidoglycan	1104:1116	arg1	presence					1060:1067	The presence	1056:1067	The presence of meso-diaminopimelic acid in the peptidoglycan and mannose, arabinose and ribose in the cell wall of strain EA-1T	1056:1183	The presence of meso-diaminopimelic acid in the peptidoglycan and mannose, arabinose and ribose in the cell wall of strain EA-1T were detected.
30234478	8	1	from	mannose	1122:1128	arg1	peptidoglycan					1104:1116	the peptidoglycan	1100:1116	the peptidoglycan	1100:1116	The presence of meso-diaminopimelic acid in the peptidoglycan and mannose, arabinose and ribose in the cell wall of strain EA-1T were detected.
30234478	6	2	theme	menaquinone	831:841	arg1	quinone					810:816	the quinone	806:816	the quinone detected	806:825	The dominant fatty acids of the novel isolate were anteiso-C17 : 0 (49.9 %), iso-C17 : 0 (23.0 %) and iso-C16 : 0 (13.3 %), while the quinone detected was menaquinone MK-7.
30234478	6	2	theme	menaquinone	831:841	arg1	MK-7					843:846	menaquinone MK-7	831:846	menaquinone MK-7	831:846	The dominant fatty acids of the novel isolate were anteiso-C17 : 0 (49.9 %), iso-C17 : 0 (23.0 %) and iso-C16 : 0 (13.3 %), while the quinone detected was menaquinone MK-7.
30234478	9	3	theme	carbon	1305:1310	arg1	source					1312:1317	the sole carbon source	1296:1317	the sole carbon source	1296:1317	The strain was able to grow heterotrophically as well as autotrophically with carbon dioxide as the sole carbon source and with hydrogen and oxygen as electron donor and acceptor, respectively.
30234478	4	4	dep	97.85 	607:612	arg1	to					604:605	to	604:605	to	604:605	The sequence similarity of the five 16S rRNA gene copies to its closest relative, Kyrpidia tusciae, ranged from 97.79 to 97.85 %.
30234478	4	5	theme	sequence	490:497	arg1	similarity					499:508	The sequence similarity	486:508	The sequence similarity of the five 16S rRNA gene copies to its closest relative, Kyrpidia tusciae,	486:584	The sequence similarity of the five 16S rRNA gene copies to its closest relative, Kyrpidia tusciae, ranged from 97.79 to 97.85 %.
30234478	8	6	theme	meso-diaminopimelic	1072:1090	arg1	acid					1092:1095	meso-diaminopimelic acid	1072:1095	meso-diaminopimelic acid in the peptidoglycan	1072:1116	The presence of meso-diaminopimelic acid in the peptidoglycan and mannose, arabinose and ribose in the cell wall of strain EA-1T were detected.
30234478	12	7	theme	type	1615:1618	arg1	strain					1620:1625	The type strain	1611:1625	The type strain	1611:1625	The type strain is strain EA-1T (=DSM 106492T=CCOS1194T).
30234478	12	7	theme	type	1615:1618	arg1	EA-1T					1637:1641	strain EA-1T	1630:1641	strain EA-1T (=DSM 106492T=CCOS1194T)	1630:1666	The type strain is strain EA-1T (=DSM 106492T=CCOS1194T).
30234478	8	8	attach	presence	1060:1067	arg2	acid					1092:1095	meso-diaminopimelic acid	1072:1095	meso-diaminopimelic acid in the peptidoglycan	1072:1116	The presence of meso-diaminopimelic acid in the peptidoglycan and mannose, arabinose and ribose in the cell wall of strain EA-1T were detected.
30234478	8	8	attach	presence	1060:1067	arg2	mannose					1122:1128	mannose	1122:1128	mannose	1122:1128	The presence of meso-diaminopimelic acid in the peptidoglycan and mannose, arabinose and ribose in the cell wall of strain EA-1T were detected.
30234478	8	8	attach	presence	1060:1067	arg1	wall					1164:1167	the cell wall	1155:1167	the cell wall of strain EA-1T	1155:1183	The presence of meso-diaminopimelic acid in the peptidoglycan and mannose, arabinose and ribose in the cell wall of strain EA-1T were detected.
30234478	10	9	theme	novel	1510:1514	arg1	species					1516:1522	a novel species	1508:1522	a novel species	1508:1522	Based on its chemotaxonomic, physiological and genomic characteristics, the new strain is considered to represent a novel species within the genus Kyrpidia, for which the name Kyrpidiaspormannii sp.
30234478	2	10	theme	sediment	310:317	arg1	samples					319:325	hydrothermal sediment samples	297:325	hydrothermal sediment samples from the Azores (São Miguel, Portugal)	297:364	A Gram-stain-positive, rod-shaped, non-motile, spore-forming bacterium, strain EA-1T, was isolated from hydrothermal sediment samples from the Azores (São Miguel, Portugal).
30234478	8	11	theme	acid	1092:1095	arg1	presence					1060:1067	The presence	1056:1067	The presence of meso-diaminopimelic acid in the peptidoglycan and mannose, arabinose and ribose in the cell wall of strain EA-1T	1056:1183	The presence of meso-diaminopimelic acid in the peptidoglycan and mannose, arabinose and ribose in the cell wall of strain EA-1T were detected.
30234478	8	12	from	arabinose	1131:1139	arg1	peptidoglycan					1104:1116	the peptidoglycan	1100:1116	the peptidoglycan	1100:1116	The presence of meso-diaminopimelic acid in the peptidoglycan and mannose, arabinose and ribose in the cell wall of strain EA-1T were detected.
30234478	3	13	with	affiliation	449:459	arg1	Kyrpidia					476:483	the genus Kyrpidia	466:483	the genus Kyrpidia	466:483	16S rRNA gene sequence analysis of the isolated bacterium revealed a phylogenetic affiliation with the genus Kyrpidia.
30234478	1	14	dep	thermophilic	32:43	arg1	facultative					66:76	facultative	66:76	facultative	66:76	nov., a thermophilic, hydrogen-oxidizing, facultative autotroph, isolated from hydrothermal systems at São Miguel Island, and emended description of the genus Kyrpidia.
30234478	1	14	dep	thermophilic	32:43	arg1	hydrogen-oxidizing					46:63	hydrogen-oxidizing	46:63	hydrogen-oxidizing	46:63	nov., a thermophilic, hydrogen-oxidizing, facultative autotroph, isolated from hydrothermal systems at São Miguel Island, and emended description of the genus Kyrpidia.
30234478	10	15	theme	genus	1535:1539	arg1	Kyrpidia					1541:1548	the genus Kyrpidia	1531:1548	the genus Kyrpidia	1531:1548	Based on its chemotaxonomic, physiological and genomic characteristics, the new strain is considered to represent a novel species within the genus Kyrpidia, for which the name Kyrpidiaspormannii sp.
30234478	1	16	theme	emended	150:156	arg1	description					158:168	emended description	150:168	emended description of the genus Kyrpidia	150:190	nov., a thermophilic, hydrogen-oxidizing, facultative autotroph, isolated from hydrothermal systems at São Miguel Island, and emended description of the genus Kyrpidia.
30234478	4	17	theme	closest	550:556	arg1	relative					558:565	its closest relative	546:565	its closest relative	546:565	The sequence similarity of the five 16S rRNA gene copies to its closest relative, Kyrpidia tusciae, ranged from 97.79 to 97.85 %.
30234478	4	17	theme	closest	550:556	arg1	tusciae					577:583	Kyrpidia tusciae	568:583	Kyrpidia tusciae	568:583	The sequence similarity of the five 16S rRNA gene copies to its closest relative, Kyrpidia tusciae, ranged from 97.79 to 97.85 %.
30234478	2	18	dep	Gram-stain-positive	195:213	arg1	rod-shaped					216:225	rod-shaped	216:225	rod-shaped	216:225	A Gram-stain-positive, rod-shaped, non-motile, spore-forming bacterium, strain EA-1T, was isolated from hydrothermal sediment samples from the Azores (São Miguel, Portugal).
30234478	2	18	dep	Gram-stain-positive	195:213	arg1	spore-forming					240:252	spore-forming	240:252	spore-forming	240:252	A Gram-stain-positive, rod-shaped, non-motile, spore-forming bacterium, strain EA-1T, was isolated from hydrothermal sediment samples from the Azores (São Miguel, Portugal).
30234478	2	18	dep	Gram-stain-positive	195:213	arg1	non-motile					228:237	non-motile	228:237	non-motile	228:237	A Gram-stain-positive, rod-shaped, non-motile, spore-forming bacterium, strain EA-1T, was isolated from hydrothermal sediment samples from the Azores (São Miguel, Portugal).
30234478	10	19	theme	Kyrpidiaspormannii	1570:1587	arg1	sp					1589:1590	the name Kyrpidiaspormannii sp	1561:1590	the name Kyrpidiaspormannii sp	1561:1590	Based on its chemotaxonomic, physiological and genomic characteristics, the new strain is considered to represent a novel species within the genus Kyrpidia, for which the name Kyrpidiaspormannii sp.
30234478	12	20	theme	=DSM	1644:1647	arg1	EA-1T					1637:1641	strain EA-1T	1630:1641	strain EA-1T (=DSM 106492T=CCOS1194T)	1630:1666	The type strain is strain EA-1T (=DSM 106492T=CCOS1194T).
30234478	12	20	theme	=DSM	1644:1647	arg1	106492T=CCOS1194T					1649:1665	=DSM 106492T=CCOS1194T	1644:1665	=DSM 106492T=CCOS1194T	1644:1665	The type strain is strain EA-1T (=DSM 106492T=CCOS1194T).
30234478	3	21	theme	isolated	406:413	arg1	bacterium					415:423	the isolated bacterium	402:423	the isolated bacterium	402:423	16S rRNA gene sequence analysis of the isolated bacterium revealed a phylogenetic affiliation with the genus Kyrpidia.
30234478	0	22	theme	spormannii	9:18	arg1	sp					20:21	Kyrpidia spormannii sp	0:21	Kyrpidia spormannii sp.	0:22	Kyrpidia spormannii sp.
30234478	4	23	theme	gene	531:534	arg1	copies					536:541	the five 16S rRNA gene copies	513:541	the five 16S rRNA gene copies	513:541	The sequence similarity of the five 16S rRNA gene copies to its closest relative, Kyrpidia tusciae, ranged from 97.79 to 97.85 %.
30234478	10	24	theme	new	1470:1472	arg1	strain					1474:1479	the new strain	1466:1479	the new strain	1466:1479	Based on its chemotaxonomic, physiological and genomic characteristics, the new strain is considered to represent a novel species within the genus Kyrpidia, for which the name Kyrpidiaspormannii sp.
30234478	2	25	from	Azores	336:341	arg1	samples					319:325	hydrothermal sediment samples	297:325	hydrothermal sediment samples from the Azores (São Miguel, Portugal)	297:364	A Gram-stain-positive, rod-shaped, non-motile, spore-forming bacterium, strain EA-1T, was isolated from hydrothermal sediment samples from the Azores (São Miguel, Portugal).
30234478	2	26	theme	hydrothermal	297:308	arg1	samples					319:325	hydrothermal sediment samples	297:325	hydrothermal sediment samples from the Azores (São Miguel, Portugal)	297:364	A Gram-stain-positive, rod-shaped, non-motile, spore-forming bacterium, strain EA-1T, was isolated from hydrothermal sediment samples from the Azores (São Miguel, Portugal).
30234478	10	27	theme	chemotaxonomic	1407:1420	arg1	characteristics					1449:1463	its chemotaxonomic, physiological and genomic characteristics	1403:1463	its chemotaxonomic, physiological and genomic characteristics	1403:1463	Based on its chemotaxonomic, physiological and genomic characteristics, the new strain is considered to represent a novel species within the genus Kyrpidia, for which the name Kyrpidiaspormannii sp.
30234478	8	28	theme	cell	1159:1162	arg1	wall					1164:1167	the cell wall	1155:1167	the cell wall of strain EA-1T	1155:1183	The presence of meso-diaminopimelic acid in the peptidoglycan and mannose, arabinose and ribose in the cell wall of strain EA-1T were detected.
30234478	0	29	theme	Kyrpidia	0:7	arg1	sp					20:21	Kyrpidia spormannii sp	0:21	Kyrpidia spormannii sp.	0:22	Kyrpidia spormannii sp.
30234478	4	30	theme	rRNA	526:529	arg1	copies					536:541	the five 16S rRNA gene copies	513:541	the five 16S rRNA gene copies	513:541	The sequence similarity of the five 16S rRNA gene copies to its closest relative, Kyrpidia tusciae, ranged from 97.79 to 97.85 %.
30234478	6	31	theme	isolate	714:720	arg1	acids					695:699	The dominant fatty acids	676:699	The dominant fatty acids of the novel isolate	676:720	The dominant fatty acids of the novel isolate were anteiso-C17 : 0 (49.9 %), iso-C17 : 0 (23.0 %) and iso-C16 : 0 (13.3 %), while the quinone detected was menaquinone MK-7.
30234478	6	31	theme	isolate	714:720	arg1	 0					740:741	 0	740:741	 0	740:741	The dominant fatty acids of the novel isolate were anteiso-C17 : 0 (49.9 %), iso-C17 : 0 (23.0 %) and iso-C16 : 0 (13.3 %), while the quinone detected was menaquinone MK-7.
30234478	3	32	theme	phylogenetic	436:447	arg1	affiliation					449:459	a phylogenetic affiliation	434:459	a phylogenetic affiliation with the genus Kyrpidia	434:483	16S rRNA gene sequence analysis of the isolated bacterium revealed a phylogenetic affiliation with the genus Kyrpidia.
30234478	4	33	theme	16S	522:524	arg1	copies					536:541	the five 16S rRNA gene copies	513:541	the five 16S rRNA gene copies	513:541	The sequence similarity of the five 16S rRNA gene copies to its closest relative, Kyrpidia tusciae, ranged from 97.79 to 97.85 %.
30234478	5	34	theme	DNA-DNA	642:648	arg1	hybridization					650:662	DNA-DNA hybridization	642:662	DNA-DNA hybridization	642:662	The in silico estimate of DNA-DNA hybridization was 56.0 %.
30234478	1	35	from	Island	138:143	arg1	systems					116:122	hydrothermal systems	103:122	hydrothermal systems at São Miguel Island	103:143	nov., a thermophilic, hydrogen-oxidizing, facultative autotroph, isolated from hydrothermal systems at São Miguel Island, and emended description of the genus Kyrpidia.
30234478	9	36	theme	carbon	1278:1283	arg1	dioxide					1285:1291	carbon dioxide	1278:1291	carbon dioxide as the sole carbon source	1278:1317	The strain was able to grow heterotrophically as well as autotrophically with carbon dioxide as the sole carbon source and with hydrogen and oxygen as electron donor and acceptor, respectively.
30234478	1	37	theme	genus	177:181	arg1	Kyrpidia					183:190	the genus Kyrpidia	173:190	the genus Kyrpidia	173:190	nov., a thermophilic, hydrogen-oxidizing, facultative autotroph, isolated from hydrothermal systems at São Miguel Island, and emended description of the genus Kyrpidia.
30234478	4	38	theme	copies	536:541	arg1	similarity					499:508	The sequence similarity	486:508	The sequence similarity of the five 16S rRNA gene copies to its closest relative, Kyrpidia tusciae,	486:584	The sequence similarity of the five 16S rRNA gene copies to its closest relative, Kyrpidia tusciae, ranged from 97.79 to 97.85 %.
30234478	7	39	theme	unidentified	971:982	arg1	compounds					984:992	additional unidentified compounds	960:992	additional unidentified compounds comprising two glycolipids, two phospholipids and two lipids	960:1053	Analysis of polar lipids identified phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and additional unidentified compounds comprising two glycolipids, two phospholipids and two lipids.
30234478	1	40	theme	Kyrpidia	183:190	arg1	autotroph					78:86	a thermophilic, hydrogen-oxidizing, facultative autotroph	30:86	a thermophilic, hydrogen-oxidizing, facultative autotroph	30:86	nov., a thermophilic, hydrogen-oxidizing, facultative autotroph, isolated from hydrothermal systems at São Miguel Island, and emended description of the genus Kyrpidia.
30234478	1	40	theme	Kyrpidia	183:190	arg1	nov.					24:27	nov.	24:27	nov.	24:27	nov., a thermophilic, hydrogen-oxidizing, facultative autotroph, isolated from hydrothermal systems at São Miguel Island, and emended description of the genus Kyrpidia.
30234478	1	40	theme	Kyrpidia	183:190	arg1	description					158:168	emended description	150:168	emended description of the genus Kyrpidia	150:190	nov., a thermophilic, hydrogen-oxidizing, facultative autotroph, isolated from hydrothermal systems at São Miguel Island, and emended description of the genus Kyrpidia.
30234478	3	41	theme	gene	376:379	arg1	analysis					390:397	16S rRNA gene sequence analysis	367:397	16S rRNA gene sequence analysis of the isolated bacterium	367:423	16S rRNA gene sequence analysis of the isolated bacterium revealed a phylogenetic affiliation with the genus Kyrpidia.
30234478	2	42	attach	isolated	283:290	arg2	bacterium					254:262	A Gram-stain-positive, rod-shaped, non-motile, spore-forming bacterium	193:262	A Gram-stain-positive, rod-shaped, non-motile, spore-forming bacterium	193:262	A Gram-stain-positive, rod-shaped, non-motile, spore-forming bacterium, strain EA-1T, was isolated from hydrothermal sediment samples from the Azores (São Miguel, Portugal).
30234478	2	42	attach	isolated	283:290	arg1	samples					319:325	hydrothermal sediment samples	297:325	hydrothermal sediment samples from the Azores (São Miguel, Portugal)	297:364	A Gram-stain-positive, rod-shaped, non-motile, spore-forming bacterium, strain EA-1T, was isolated from hydrothermal sediment samples from the Azores (São Miguel, Portugal).
30234478	2	42	attach	isolated	283:290	arg2	strain					265:270	strain EA-1T	265:276	strain EA-1T	265:276	A Gram-stain-positive, rod-shaped, non-motile, spore-forming bacterium, strain EA-1T, was isolated from hydrothermal sediment samples from the Azores (São Miguel, Portugal).
30234478	8	43	from	ribose	1145:1150	arg1	peptidoglycan					1104:1116	the peptidoglycan	1100:1116	the peptidoglycan	1100:1116	The presence of meso-diaminopimelic acid in the peptidoglycan and mannose, arabinose and ribose in the cell wall of strain EA-1T were detected.
30234478	8	44	theme	EA-1T	1179:1183	arg1	wall					1164:1167	the cell wall	1155:1167	the cell wall of strain EA-1T	1155:1183	The presence of meso-diaminopimelic acid in the peptidoglycan and mannose, arabinose and ribose in the cell wall of strain EA-1T were detected.
30234478	10	45	theme	name	1565:1568	arg1	sp					1589:1590	the name Kyrpidiaspormannii sp	1561:1590	the name Kyrpidiaspormannii sp	1561:1590	Based on its chemotaxonomic, physiological and genomic characteristics, the new strain is considered to represent a novel species within the genus Kyrpidia, for which the name Kyrpidiaspormannii sp.
30234478	3	46	theme	bacterium	415:423	arg1	analysis					390:397	16S rRNA gene sequence analysis	367:397	16S rRNA gene sequence analysis of the isolated bacterium	367:423	16S rRNA gene sequence analysis of the isolated bacterium revealed a phylogenetic affiliation with the genus Kyrpidia.
30234478	3	47	theme	sequence	381:388	arg1	analysis					390:397	16S rRNA gene sequence analysis	367:397	16S rRNA gene sequence analysis of the isolated bacterium	367:423	16S rRNA gene sequence analysis of the isolated bacterium revealed a phylogenetic affiliation with the genus Kyrpidia.
30234478	8	48	theme	mannose	1122:1128	arg1	presence					1060:1067	The presence	1056:1067	The presence of meso-diaminopimelic acid in the peptidoglycan and mannose, arabinose and ribose in the cell wall of strain EA-1T	1056:1183	The presence of meso-diaminopimelic acid in the peptidoglycan and mannose, arabinose and ribose in the cell wall of strain EA-1T were detected.
30234478	7	49	theme	lipids	867:872	arg1	Analysis					849:856	Analysis	849:856	Analysis of polar lipids	849:872	Analysis of polar lipids identified phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and additional unidentified compounds comprising two glycolipids, two phospholipids and two lipids.
30234478	8	50	theme	strain	1172:1177	arg1	EA-1T					1179:1183	strain EA-1T	1172:1183	strain EA-1T	1172:1183	The presence of meso-diaminopimelic acid in the peptidoglycan and mannose, arabinose and ribose in the cell wall of strain EA-1T were detected.
30234478	3	51	theme	16S	367:369	arg1	analysis					390:397	16S rRNA gene sequence analysis	367:397	16S rRNA gene sequence analysis of the isolated bacterium	367:423	16S rRNA gene sequence analysis of the isolated bacterium revealed a phylogenetic affiliation with the genus Kyrpidia.
30234478	6	52	theme	novel	708:712	arg1	isolate					714:720	the novel isolate	704:720	the novel isolate	704:720	The dominant fatty acids of the novel isolate were anteiso-C17 : 0 (49.9 %), iso-C17 : 0 (23.0 %) and iso-C16 : 0 (13.3 %), while the quinone detected was menaquinone MK-7.
30234478	1	53	theme	hydrothermal	103:114	arg1	systems					116:122	hydrothermal systems	103:122	hydrothermal systems at São Miguel Island	103:143	nov., a thermophilic, hydrogen-oxidizing, facultative autotroph, isolated from hydrothermal systems at São Miguel Island, and emended description of the genus Kyrpidia.
30234478	3	54	theme	rRNA	371:374	arg1	analysis					390:397	16S rRNA gene sequence analysis	367:397	16S rRNA gene sequence analysis of the isolated bacterium	367:423	16S rRNA gene sequence analysis of the isolated bacterium revealed a phylogenetic affiliation with the genus Kyrpidia.
30234478	7	55	theme	additional	960:969	arg1	compounds					984:992	additional unidentified compounds	960:992	additional unidentified compounds comprising two glycolipids, two phospholipids and two lipids	960:1053	Analysis of polar lipids identified phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and additional unidentified compounds comprising two glycolipids, two phospholipids and two lipids.
30234478	8	56	from	acid	1092:1095	arg1	peptidoglycan					1104:1116	the peptidoglycan	1100:1116	the peptidoglycan	1100:1116	The presence of meso-diaminopimelic acid in the peptidoglycan and mannose, arabinose and ribose in the cell wall of strain EA-1T were detected.
30234478	2	57	theme	Gram-stain-positive	195:213	arg1	strain					265:270	strain EA-1T	265:276	strain EA-1T	265:276	A Gram-stain-positive, rod-shaped, non-motile, spore-forming bacterium, strain EA-1T, was isolated from hydrothermal sediment samples from the Azores (São Miguel, Portugal).
30234478	2	57	theme	Gram-stain-positive	195:213	arg1	bacterium					254:262	A Gram-stain-positive, rod-shaped, non-motile, spore-forming bacterium	193:262	A Gram-stain-positive, rod-shaped, non-motile, spore-forming bacterium	193:262	A Gram-stain-positive, rod-shaped, non-motile, spore-forming bacterium, strain EA-1T, was isolated from hydrothermal sediment samples from the Azores (São Miguel, Portugal).
30234478	3	58	theme	genus	470:474	arg1	Kyrpidia					476:483	the genus Kyrpidia	466:483	the genus Kyrpidia	466:483	16S rRNA gene sequence analysis of the isolated bacterium revealed a phylogenetic affiliation with the genus Kyrpidia.
30234478	9	59	dep	grow	1223:1226	arg1	dioxide					1285:1291	carbon dioxide	1278:1291	carbon dioxide as the sole carbon source	1278:1317	The strain was able to grow heterotrophically as well as autotrophically with carbon dioxide as the sole carbon source and with hydrogen and oxygen as electron donor and acceptor, respectively.
30234478	9	59	dep	grow	1223:1226	arg1	heterotrophically					1228:1244	heterotrophically	1228:1244	heterotrophically	1228:1244	The strain was able to grow heterotrophically as well as autotrophically with carbon dioxide as the sole carbon source and with hydrogen and oxygen as electron donor and acceptor, respectively.
30234478	1	60	attach	isolated	89:96	arg2	autotroph					78:86	a thermophilic, hydrogen-oxidizing, facultative autotroph	30:86	a thermophilic, hydrogen-oxidizing, facultative autotroph	30:86	nov., a thermophilic, hydrogen-oxidizing, facultative autotroph, isolated from hydrothermal systems at São Miguel Island, and emended description of the genus Kyrpidia.
30234478	1	60	attach	isolated	89:96	arg1	systems					116:122	hydrothermal systems	103:122	hydrothermal systems at São Miguel Island	103:143	nov., a thermophilic, hydrogen-oxidizing, facultative autotroph, isolated from hydrothermal systems at São Miguel Island, and emended description of the genus Kyrpidia.
30234478	6	61	theme	fatty	689:693	arg1	acids					695:699	The dominant fatty acids	676:699	The dominant fatty acids of the novel isolate	676:720	The dominant fatty acids of the novel isolate were anteiso-C17 : 0 (49.9 %), iso-C17 : 0 (23.0 %) and iso-C16 : 0 (13.3 %), while the quinone detected was menaquinone MK-7.
30234478	6	61	theme	fatty	689:693	arg1	 0					740:741	 0	740:741	 0	740:741	The dominant fatty acids of the novel isolate were anteiso-C17 : 0 (49.9 %), iso-C17 : 0 (23.0 %) and iso-C16 : 0 (13.3 %), while the quinone detected was menaquinone MK-7.
30234478	7	62	theme	polar	861:865	arg1	lipids					867:872	polar lipids	861:872	polar lipids	861:872	Analysis of polar lipids identified phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and additional unidentified compounds comprising two glycolipids, two phospholipids and two lipids.
30234478	6	63	theme	dominant	680:687	arg1	acids					695:699	The dominant fatty acids	676:699	The dominant fatty acids of the novel isolate	676:720	The dominant fatty acids of the novel isolate were anteiso-C17 : 0 (49.9 %), iso-C17 : 0 (23.0 %) and iso-C16 : 0 (13.3 %), while the quinone detected was menaquinone MK-7.
30234478	6	63	theme	dominant	680:687	arg1	 0					740:741	 0	740:741	 0	740:741	The dominant fatty acids of the novel isolate were anteiso-C17 : 0 (49.9 %), iso-C17 : 0 (23.0 %) and iso-C16 : 0 (13.3 %), while the quinone detected was menaquinone MK-7.
30234478	1	64	theme	São	127:129	arg1	Island					138:143	São Miguel Island	127:143	São Miguel Island	127:143	nov., a thermophilic, hydrogen-oxidizing, facultative autotroph, isolated from hydrothermal systems at São Miguel Island, and emended description of the genus Kyrpidia.
30234478	8	65	from	presence	1060:1067	arg1	peptidoglycan					1104:1116	the peptidoglycan	1100:1116	the peptidoglycan	1100:1116	The presence of meso-diaminopimelic acid in the peptidoglycan and mannose, arabinose and ribose in the cell wall of strain EA-1T were detected.
30234478	8	65	from	presence	1060:1067	arg1	wall					1164:1167	the cell wall	1155:1167	the cell wall of strain EA-1T	1155:1183	The presence of meso-diaminopimelic acid in the peptidoglycan and mannose, arabinose and ribose in the cell wall of strain EA-1T were detected.
30234478	9	66	theme	electron	1351:1358	arg1	donor					1360:1364	electron donor	1351:1364	electron donor	1351:1364	The strain was able to grow heterotrophically as well as autotrophically with carbon dioxide as the sole carbon source and with hydrogen and oxygen as electron donor and acceptor, respectively.
30234478	1	67	theme	Miguel	131:136	arg1	Island					138:143	São Miguel Island	127:143	São Miguel Island	127:143	nov., a thermophilic, hydrogen-oxidizing, facultative autotroph, isolated from hydrothermal systems at São Miguel Island, and emended description of the genus Kyrpidia.
30234478	12	68	theme	strain	1630:1635	arg1	strain					1620:1625	The type strain	1611:1625	The type strain	1611:1625	The type strain is strain EA-1T (=DSM 106492T=CCOS1194T).
30234478	12	68	theme	strain	1630:1635	arg1	EA-1T					1637:1641	strain EA-1T	1630:1641	strain EA-1T (=DSM 106492T=CCOS1194T)	1630:1666	The type strain is strain EA-1T (=DSM 106492T=CCOS1194T).
30234478	12	68	theme	strain	1630:1635	arg1	106492T=CCOS1194T					1649:1665	=DSM 106492T=CCOS1194T	1644:1665	=DSM 106492T=CCOS1194T	1644:1665	The type strain is strain EA-1T (=DSM 106492T=CCOS1194T).
30234478	10	69	theme	physiological	1423:1435	arg1	characteristics					1449:1463	its chemotaxonomic, physiological and genomic characteristics	1403:1463	its chemotaxonomic, physiological and genomic characteristics	1403:1463	Based on its chemotaxonomic, physiological and genomic characteristics, the new strain is considered to represent a novel species within the genus Kyrpidia, for which the name Kyrpidiaspormannii sp.
30234478	10	70	theme	genomic	1441:1447	arg1	characteristics					1449:1463	its chemotaxonomic, physiological and genomic characteristics	1403:1463	its chemotaxonomic, physiological and genomic characteristics	1403:1463	Based on its chemotaxonomic, physiological and genomic characteristics, the new strain is considered to represent a novel species within the genus Kyrpidia, for which the name Kyrpidiaspormannii sp.
30234478	1	71	theme	thermophilic	32:43	arg1	autotroph					78:86	a thermophilic, hydrogen-oxidizing, facultative autotroph	30:86	a thermophilic, hydrogen-oxidizing, facultative autotroph	30:86	nov., a thermophilic, hydrogen-oxidizing, facultative autotroph, isolated from hydrothermal systems at São Miguel Island, and emended description of the genus Kyrpidia.
30234478	9	72	theme	sole	1300:1303	arg1	source					1312:1317	the sole carbon source	1296:1317	the sole carbon source	1296:1317	The strain was able to grow heterotrophically as well as autotrophically with carbon dioxide as the sole carbon source and with hydrogen and oxygen as electron donor and acceptor, respectively.
29975568	10	0	from	status	1927:1932	arg1	pigs					1961:1964	high-fat diet-fed adult pigs	1937:1964	high-fat diet-fed adult pigs	1937:1964	Our results highlight the key role of perinatal nutrition on later microbiota and host metabolic adaptation to an unbalanced diet.-Le Bourgot, C., Ferret-Bernard, S., Apper, E., Taminiau, B., Cahu, A., Le Normand, L., Respondek, F., Le Huërou-Luron, I., Blat, S. Perinatal short-chain fructooligosaccharides program intestinal microbiota and improve enteroinsular axis function and inflammatory status in high-fat diet-fed adult pigs.
29975568	4	1	theme	gestation	646:654	arg1	end					639:641	the end	635:641	the end of gestation and the entire lactation	635:679	Sows were supplemented with scFOS or not during the end of gestation and the entire lactation, and offspring received scFOS accordingly during 1 mo after weaning.
29975568	6	2	theme	fecal	935:939	arg1	microbiota					941:950	the fecal microbiota	931:950	the fecal microbiota	931:950	Perinatal scFOS supplementation induced a persistent modulation of the composition of the fecal microbiota in adulthood, notably by increasing the Prevotella genus.
29975568	7	3	theme	peripheral	1166:1175	arg1	sensitivity					1185:1195	peripheral insulin sensitivity	1166:1195	peripheral insulin sensitivity	1166:1195	Meanwhile, scFOS animals displayed improved capacity to secrete glucagon-like peptide-1 and improved pancreas sensitivity to glucose without any changes in peripheral insulin sensitivity.
29975568	8	4	theme	secretory	1251:1259	arg1	secretion					1265:1273	ileal secretory IgA secretion	1245:1273	ileal secretory IgA secretion	1245:1273	Perinatal scFOS supplementation also increased ileal secretory IgA secretion and alkaline phosphatase activity and decreased TNF-α expression in adipose tissue.
29975568	1	5	theme	metabolic	217:225	arg1	functions					227:235	physiologic and metabolic functions	201:235	physiologic and metabolic functions	201:235	Perinatal nutrition programs physiologic and metabolic functions, with consequences on the susceptibility to develop metabolic diseases in adulthood.
29975568	8	6	theme	adipose	1343:1349	arg1	tissue					1351:1356	adipose tissue	1343:1356	adipose tissue	1343:1356	Perinatal scFOS supplementation also increased ileal secretory IgA secretion and alkaline phosphatase activity and decreased TNF-α expression in adipose tissue.
29975568	8	7	theme	alkaline	1279:1286	arg1	phosphatase					1288:1298	alkaline phosphatase	1279:1298	alkaline phosphatase activity	1279:1307	Perinatal scFOS supplementation also increased ileal secretory IgA secretion and alkaline phosphatase activity and decreased TNF-α expression in adipose tissue.
29975568	0	8	theme	diet-fed	151:158	arg1	pigs					166:169	high-fat diet-fed adult pigs	142:169	high-fat diet-fed adult pigs	142:169	Perinatal short-chain fructooligosaccharides program intestinal microbiota and improve enteroinsular axis function and inflammatory status in high-fat diet-fed adult pigs.
29975568	2	9	theme	key	350:352	arg1	factor					354:359	a key factor	348:359	a key factor of such programming	348:379	The microbiota represents a key factor of such programming.
29975568	10	10	from	role	1562:1565	arg1	microbiota					1599:1608	later microbiota	1593:1608	later microbiota	1593:1608	Our results highlight the key role of perinatal nutrition on later microbiota and host metabolic adaptation to an unbalanced diet.-Le Bourgot, C., Ferret-Bernard, S., Apper, E., Taminiau, B., Cahu, A., Le Normand, L., Respondek, F., Le Huërou-Luron, I., Blat, S. Perinatal short-chain fructooligosaccharides program intestinal microbiota and improve enteroinsular axis function and inflammatory status in high-fat diet-fed adult pigs.
29975568	10	10	from	role	1562:1565	arg1	program					1840:1846	host metabolic adaptation to an unbalanced diet.-Le Bourgot, C., Ferret-Bernard, S., Apper, E., Taminiau, B., Cahu, A., Le Normand, L., Respondek, F., Le Huërou-Luron, I., Blat, S. Perinatal short-chain fructooligosaccharides program	1614:1846	host metabolic adaptation to an unbalanced diet.-Le Bourgot, C., Ferret-Bernard, S., Apper, E., Taminiau, B., Cahu, A., Le Normand, L., Respondek, F., Le Huërou-Luron, I., Blat, S. Perinatal short-chain fructooligosaccharides program	1614:1846	Our results highlight the key role of perinatal nutrition on later microbiota and host metabolic adaptation to an unbalanced diet.-Le Bourgot, C., Ferret-Bernard, S., Apper, E., Taminiau, B., Cahu, A., Le Normand, L., Respondek, F., Le Huërou-Luron, I., Blat, S. Perinatal short-chain fructooligosaccharides program intestinal microbiota and improve enteroinsular axis function and inflammatory status in high-fat diet-fed adult pigs.
29975568	0	11	from	function	106:113	arg1	pigs					166:169	high-fat diet-fed adult pigs	142:169	high-fat diet-fed adult pigs	142:169	Perinatal short-chain fructooligosaccharides program intestinal microbiota and improve enteroinsular axis function and inflammatory status in high-fat diet-fed adult pigs.
29975568	7	12	theme	pancreas	1111:1118	arg1	sensitivity					1120:1130	pancreas sensitivity	1111:1130	pancreas sensitivity to glucose	1111:1141	Meanwhile, scFOS animals displayed improved capacity to secrete glucagon-like peptide-1 and improved pancreas sensitivity to glucose without any changes in peripheral insulin sensitivity.
29975568	10	13	theme	intestinal	1848:1857	arg1	microbiota					1859:1868	intestinal microbiota	1848:1868	intestinal microbiota	1848:1868	Our results highlight the key role of perinatal nutrition on later microbiota and host metabolic adaptation to an unbalanced diet.-Le Bourgot, C., Ferret-Bernard, S., Apper, E., Taminiau, B., Cahu, A., Le Normand, L., Respondek, F., Le Huërou-Luron, I., Blat, S. Perinatal short-chain fructooligosaccharides program intestinal microbiota and improve enteroinsular axis function and inflammatory status in high-fat diet-fed adult pigs.
29975568	8	14	from	expression	1329:1338	arg1	tissue					1351:1356	adipose tissue	1343:1356	adipose tissue	1343:1356	Perinatal scFOS supplementation also increased ileal secretory IgA secretion and alkaline phosphatase activity and decreased TNF-α expression in adipose tissue.
29975568	7	15	theme	glucagon-like	1074:1086	arg1	peptide-1					1088:1096	glucagon-like peptide-1	1074:1096	glucagon-like peptide-1	1074:1096	Meanwhile, scFOS animals displayed improved capacity to secrete glucagon-like peptide-1 and improved pancreas sensitivity to glucose without any changes in peripheral insulin sensitivity.
29975568	10	16	theme	enteroinsular	1882:1894	arg1	function					1901:1908	enteroinsular axis function	1882:1908	enteroinsular axis function	1882:1908	Our results highlight the key role of perinatal nutrition on later microbiota and host metabolic adaptation to an unbalanced diet.-Le Bourgot, C., Ferret-Bernard, S., Apper, E., Taminiau, B., Cahu, A., Le Normand, L., Respondek, F., Le Huërou-Luron, I., Blat, S. Perinatal short-chain fructooligosaccharides program intestinal microbiota and improve enteroinsular axis function and inflammatory status in high-fat diet-fed adult pigs.
29975568	0	17	theme	enteroinsular	87:99	arg1	function					106:113	enteroinsular axis function	87:113	enteroinsular axis function	87:113	Perinatal short-chain fructooligosaccharides program intestinal microbiota and improve enteroinsular axis function and inflammatory status in high-fat diet-fed adult pigs.
29975568	7	18	theme	Meanwhile	1010:1018	arg1	animals					1027:1033	Meanwhile, scFOS animals	1010:1033	Meanwhile, scFOS animals	1010:1033	Meanwhile, scFOS animals displayed improved capacity to secrete glucagon-like peptide-1 and improved pancreas sensitivity to glucose without any changes in peripheral insulin sensitivity.
29975568	3	19	with	association	522:532	arg1	changes					550:556	microbiota changes	539:556	microbiota changes in pigs used as human model	539:584	We investigated whether perinatal prebiotic [short-chain fructooligosaccharides (scFOS)] supplementation improved adult metabolic health in association with microbiota changes in pigs used as human model.
29975568	9	20	theme	scFOS	1384:1388	arg1	supplementation					1390:1404	perinatal scFOS supplementation	1374:1404	perinatal scFOS supplementation	1374:1404	In conclusion, perinatal scFOS supplementation induced long-lasting modulation of intestinal microbiota and had beneficial consequences on the host physiology in adulthood.
29975568	1	21	theme	physiologic	201:211	arg1	functions					227:235	physiologic and metabolic functions	201:235	physiologic and metabolic functions	201:235	Perinatal nutrition programs physiologic and metabolic functions, with consequences on the susceptibility to develop metabolic diseases in adulthood.
29975568	3	22	theme	prebiotic	416:424	arg1	supplementation					471:485	perinatal prebiotic [short-chain fructooligosaccharides (scFOS)] supplementation	406:485	perinatal prebiotic [short-chain fructooligosaccharides (scFOS)] supplementation	406:485	We investigated whether perinatal prebiotic [short-chain fructooligosaccharides (scFOS)] supplementation improved adult metabolic health in association with microbiota changes in pigs used as human model.
29975568	10	23	theme	key	1558:1560	arg1	role					1562:1565	the key role	1554:1565	the key role of perinatal nutrition on later microbiota and host metabolic adaptation to an unbalanced diet.-Le Bourgot, C., Ferret-Bernard, S., Apper, E., Taminiau, B., Cahu, A., Le Normand, L., Respondek, F., Le Huërou-Luron, I., Blat, S. Perinatal short-chain fructooligosaccharides program intestinal microbiota	1554:1868	Our results highlight the key role of perinatal nutrition on later microbiota and host metabolic adaptation to an unbalanced diet.-Le Bourgot, C., Ferret-Bernard, S., Apper, E., Taminiau, B., Cahu, A., Le Normand, L., Respondek, F., Le Huërou-Luron, I., Blat, S. Perinatal short-chain fructooligosaccharides program intestinal microbiota and improve enteroinsular axis function and inflammatory status in high-fat diet-fed adult pigs.
29975568	0	24	theme	inflammatory	119:130	arg1	status					132:137	inflammatory status	119:137	inflammatory status	119:137	Perinatal short-chain fructooligosaccharides program intestinal microbiota and improve enteroinsular axis function and inflammatory status in high-fat diet-fed adult pigs.
29975568	10	25	theme	later	1593:1597	arg1	microbiota					1599:1608	later microbiota	1593:1608	later microbiota	1593:1608	Our results highlight the key role of perinatal nutrition on later microbiota and host metabolic adaptation to an unbalanced diet.-Le Bourgot, C., Ferret-Bernard, S., Apper, E., Taminiau, B., Cahu, A., Le Normand, L., Respondek, F., Le Huërou-Luron, I., Blat, S. Perinatal short-chain fructooligosaccharides program intestinal microbiota and improve enteroinsular axis function and inflammatory status in high-fat diet-fed adult pigs.
29975568	3	26	theme	scFOS	463:467	arg1	supplementation					471:485	perinatal prebiotic [short-chain fructooligosaccharides (scFOS)] supplementation	406:485	perinatal prebiotic [short-chain fructooligosaccharides (scFOS)] supplementation	406:485	We investigated whether perinatal prebiotic [short-chain fructooligosaccharides (scFOS)] supplementation improved adult metabolic health in association with microbiota changes in pigs used as human model.
29975568	10	27	theme	nutrition	1580:1588	arg1	role					1562:1565	the key role	1554:1565	the key role of perinatal nutrition on later microbiota and host metabolic adaptation to an unbalanced diet.-Le Bourgot, C., Ferret-Bernard, S., Apper, E., Taminiau, B., Cahu, A., Le Normand, L., Respondek, F., Le Huërou-Luron, I., Blat, S. Perinatal short-chain fructooligosaccharides program intestinal microbiota	1554:1868	Our results highlight the key role of perinatal nutrition on later microbiota and host metabolic adaptation to an unbalanced diet.-Le Bourgot, C., Ferret-Bernard, S., Apper, E., Taminiau, B., Cahu, A., Le Normand, L., Respondek, F., Le Huërou-Luron, I., Blat, S. Perinatal short-chain fructooligosaccharides program intestinal microbiota and improve enteroinsular axis function and inflammatory status in high-fat diet-fed adult pigs.
29975568	3	28	theme	metabolic	502:510	arg1	health					512:517	adult metabolic health	496:517	adult metabolic health	496:517	We investigated whether perinatal prebiotic [short-chain fructooligosaccharides (scFOS)] supplementation improved adult metabolic health in association with microbiota changes in pigs used as human model.
29975568	0	29	theme	Perinatal	0:8	arg1	short-chain					10:20	Perinatal short-chain	0:20	Perinatal short-chain	0:20	Perinatal short-chain fructooligosaccharides program intestinal microbiota and improve enteroinsular axis function and inflammatory status in high-fat diet-fed adult pigs.
29975568	9	30	theme	intestinal	1441:1450	arg1	microbiota					1452:1461	intestinal microbiota	1441:1461	intestinal microbiota	1441:1461	In conclusion, perinatal scFOS supplementation induced long-lasting modulation of intestinal microbiota and had beneficial consequences on the host physiology in adulthood.
29975568	3	31	theme	fructooligosaccharides	439:460	arg1	supplementation					471:485	perinatal prebiotic [short-chain fructooligosaccharides (scFOS)] supplementation	406:485	perinatal prebiotic [short-chain fructooligosaccharides (scFOS)] supplementation	406:485	We investigated whether perinatal prebiotic [short-chain fructooligosaccharides (scFOS)] supplementation improved adult metabolic health in association with microbiota changes in pigs used as human model.
29975568	10	32	theme	metabolic	1619:1627	arg1	adaptation					1629:1638	host metabolic adaptation to an unbalanced diet.-Le Bourgot, C., Ferret-Bernard, S., Apper, E., Taminiau, B., Cahu, A., Le Normand, L., Respondek, F., Le Huërou-Luron, I., Blat, S. Perinatal short-chain fructooligosaccharides	1614:1838	host metabolic adaptation to an unbalanced diet.-Le Bourgot, C., Ferret-Bernard, S., Apper, E., Taminiau, B., Cahu, A., Le Normand, L., Respondek, F., Le Huërou-Luron, I., Blat, S. Perinatal short-chain fructooligosaccharides program	1614:1846	Our results highlight the key role of perinatal nutrition on later microbiota and host metabolic adaptation to an unbalanced diet.-Le Bourgot, C., Ferret-Bernard, S., Apper, E., Taminiau, B., Cahu, A., Le Normand, L., Respondek, F., Le Huërou-Luron, I., Blat, S. Perinatal short-chain fructooligosaccharides program intestinal microbiota and improve enteroinsular axis function and inflammatory status in high-fat diet-fed adult pigs.
29975568	7	33	from	changes	1155:1161	arg1	sensitivity					1185:1195	peripheral insulin sensitivity	1166:1195	peripheral insulin sensitivity	1166:1195	Meanwhile, scFOS animals displayed improved capacity to secrete glucagon-like peptide-1 and improved pancreas sensitivity to glucose without any changes in peripheral insulin sensitivity.
29975568	10	34	theme	inflammatory	1914:1925	arg1	status					1927:1932	inflammatory status	1914:1932	inflammatory status	1914:1932	Our results highlight the key role of perinatal nutrition on later microbiota and host metabolic adaptation to an unbalanced diet.-Le Bourgot, C., Ferret-Bernard, S., Apper, E., Taminiau, B., Cahu, A., Le Normand, L., Respondek, F., Le Huërou-Luron, I., Blat, S. Perinatal short-chain fructooligosaccharides program intestinal microbiota and improve enteroinsular axis function and inflammatory status in high-fat diet-fed adult pigs.
29975568	0	35	theme	intestinal	53:62	arg1	microbiota					64:73	program intestinal microbiota	45:73	program intestinal microbiota	45:73	Perinatal short-chain fructooligosaccharides program intestinal microbiota and improve enteroinsular axis function and inflammatory status in high-fat diet-fed adult pigs.
29975568	9	36	theme	beneficial	1471:1480	arg1	consequences					1482:1493	beneficial consequences	1471:1493	beneficial consequences	1471:1493	In conclusion, perinatal scFOS supplementation induced long-lasting modulation of intestinal microbiota and had beneficial consequences on the host physiology in adulthood.
29975568	6	37	theme	persistent	887:896	arg1	modulation					898:907	a persistent modulation	885:907	a persistent modulation of the composition of the fecal microbiota	885:950	Perinatal scFOS supplementation induced a persistent modulation of the composition of the fecal microbiota in adulthood, notably by increasing the Prevotella genus.
29975568	10	38	theme	diet-fed	1946:1953	arg1	pigs					1961:1964	high-fat diet-fed adult pigs	1937:1964	high-fat diet-fed adult pigs	1937:1964	Our results highlight the key role of perinatal nutrition on later microbiota and host metabolic adaptation to an unbalanced diet.-Le Bourgot, C., Ferret-Bernard, S., Apper, E., Taminiau, B., Cahu, A., Le Normand, L., Respondek, F., Le Huërou-Luron, I., Blat, S. Perinatal short-chain fructooligosaccharides program intestinal microbiota and improve enteroinsular axis function and inflammatory status in high-fat diet-fed adult pigs.
29975568	9	39	theme	host	1502:1505	arg1	physiology					1507:1516	the host physiology	1498:1516	the host physiology in adulthood	1498:1529	In conclusion, perinatal scFOS supplementation induced long-lasting modulation of intestinal microbiota and had beneficial consequences on the host physiology in adulthood.
29975568	9	40	from	physiology	1507:1516	arg1	adulthood					1521:1529	adulthood	1521:1529	adulthood	1521:1529	In conclusion, perinatal scFOS supplementation induced long-lasting modulation of intestinal microbiota and had beneficial consequences on the host physiology in adulthood.
29975568	6	41	theme	scFOS	855:859	arg1	supplementation					861:875	Perinatal scFOS supplementation	845:875	Perinatal scFOS supplementation	845:875	Perinatal scFOS supplementation induced a persistent modulation of the composition of the fecal microbiota in adulthood, notably by increasing the Prevotella genus.
29975568	4	42	theme	entire	664:669	arg1	lactation					671:679	the entire lactation	660:679	the entire lactation	660:679	Sows were supplemented with scFOS or not during the end of gestation and the entire lactation, and offspring received scFOS accordingly during 1 mo after weaning.
29975568	10	43	from	function	1901:1908	arg1	pigs					1961:1964	high-fat diet-fed adult pigs	1937:1964	high-fat diet-fed adult pigs	1937:1964	Our results highlight the key role of perinatal nutrition on later microbiota and host metabolic adaptation to an unbalanced diet.-Le Bourgot, C., Ferret-Bernard, S., Apper, E., Taminiau, B., Cahu, A., Le Normand, L., Respondek, F., Le Huërou-Luron, I., Blat, S. Perinatal short-chain fructooligosaccharides program intestinal microbiota and improve enteroinsular axis function and inflammatory status in high-fat diet-fed adult pigs.
29975568	7	44	theme	insulin	1177:1183	arg1	sensitivity					1185:1195	peripheral insulin sensitivity	1166:1195	peripheral insulin sensitivity	1166:1195	Meanwhile, scFOS animals displayed improved capacity to secrete glucagon-like peptide-1 and improved pancreas sensitivity to glucose without any changes in peripheral insulin sensitivity.
29975568	8	45	theme	scFOS	1208:1212	arg1	supplementation					1214:1228	Perinatal scFOS supplementation	1198:1228	Perinatal scFOS supplementation	1198:1228	Perinatal scFOS supplementation also increased ileal secretory IgA secretion and alkaline phosphatase activity and decreased TNF-α expression in adipose tissue.
29975568	6	46	theme	microbiota	941:950	arg1	composition					916:926	the composition	912:926	the composition of the fecal microbiota	912:950	Perinatal scFOS supplementation induced a persistent modulation of the composition of the fecal microbiota in adulthood, notably by increasing the Prevotella genus.
29975568	8	47	theme	ileal	1245:1249	arg1	secretion					1265:1273	ileal secretory IgA secretion	1245:1273	ileal secretory IgA secretion	1245:1273	Perinatal scFOS supplementation also increased ileal secretory IgA secretion and alkaline phosphatase activity and decreased TNF-α expression in adipose tissue.
29975568	6	48	theme	composition	916:926	arg1	modulation					898:907	a persistent modulation	885:907	a persistent modulation of the composition of the fecal microbiota	885:950	Perinatal scFOS supplementation induced a persistent modulation of the composition of the fecal microbiota in adulthood, notably by increasing the Prevotella genus.
29975568	8	49	theme	TNF-α	1323:1327	arg1	expression					1329:1338	TNF-α expression	1323:1338	TNF-α expression in adipose tissue	1323:1356	Perinatal scFOS supplementation also increased ileal secretory IgA secretion and alkaline phosphatase activity and decreased TNF-α expression in adipose tissue.
29975568	2	50	theme	such	364:367	arg1	programming					369:379	such programming	364:379	such programming	364:379	The microbiota represents a key factor of such programming.
29975568	8	51	theme	IgA	1261:1263	arg1	secretion					1265:1273	ileal secretory IgA secretion	1245:1273	ileal secretory IgA secretion	1245:1273	Perinatal scFOS supplementation also increased ileal secretory IgA secretion and alkaline phosphatase activity and decreased TNF-α expression in adipose tissue.
29975568	0	52	theme	adult	160:164	arg1	pigs					166:169	high-fat diet-fed adult pigs	142:169	high-fat diet-fed adult pigs	142:169	Perinatal short-chain fructooligosaccharides program intestinal microbiota and improve enteroinsular axis function and inflammatory status in high-fat diet-fed adult pigs.
29975568	6	53	theme	Prevotella	992:1001	arg1	genus					1003:1007	the Prevotella genus	988:1007	the Prevotella genus	988:1007	Perinatal scFOS supplementation induced a persistent modulation of the composition of the fecal microbiota in adulthood, notably by increasing the Prevotella genus.
29975568	1	54	from	consequences	243:254	arg1	susceptibility					263:276	the susceptibility	259:276	the susceptibility to develop metabolic diseases in adulthood	259:319	Perinatal nutrition programs physiologic and metabolic functions, with consequences on the susceptibility to develop metabolic diseases in adulthood.
29975568	1	55	from	diseases	299:306	arg1	adulthood					311:319	adulthood	311:319	adulthood	311:319	Perinatal nutrition programs physiologic and metabolic functions, with consequences on the susceptibility to develop metabolic diseases in adulthood.
29975568	8	56	theme	phosphatase	1288:1298	arg1	activity					1300:1307	alkaline phosphatase activity	1279:1307	alkaline phosphatase activity	1279:1307	Perinatal scFOS supplementation also increased ileal secretory IgA secretion and alkaline phosphatase activity and decreased TNF-α expression in adipose tissue.
29975568	10	57	theme	axis	1896:1899	arg1	function					1901:1908	enteroinsular axis function	1882:1908	enteroinsular axis function	1882:1908	Our results highlight the key role of perinatal nutrition on later microbiota and host metabolic adaptation to an unbalanced diet.-Le Bourgot, C., Ferret-Bernard, S., Apper, E., Taminiau, B., Cahu, A., Le Normand, L., Respondek, F., Le Huërou-Luron, I., Blat, S. Perinatal short-chain fructooligosaccharides program intestinal microbiota and improve enteroinsular axis function and inflammatory status in high-fat diet-fed adult pigs.
29975568	7	58	theme	improved	1045:1052	arg1	capacity					1054:1061	improved capacity to secrete glucagon-like peptide-1 and improved pancreas sensitivity to glucose without any changes in peripheral insulin sensitivity	1045:1195	improved capacity to secrete glucagon-like peptide-1 and improved pancreas sensitivity to glucose without any changes in peripheral insulin sensitivity	1045:1195	Meanwhile, scFOS animals displayed improved capacity to secrete glucagon-like peptide-1 and improved pancreas sensitivity to glucose without any changes in peripheral insulin sensitivity.
29975568	0	59	from	status	132:137	arg1	pigs					166:169	high-fat diet-fed adult pigs	142:169	high-fat diet-fed adult pigs	142:169	Perinatal short-chain fructooligosaccharides program intestinal microbiota and improve enteroinsular axis function and inflammatory status in high-fat diet-fed adult pigs.
29975568	0	60	theme	axis	101:104	arg1	function					106:113	enteroinsular axis function	87:113	enteroinsular axis function	87:113	Perinatal short-chain fructooligosaccharides program intestinal microbiota and improve enteroinsular axis function and inflammatory status in high-fat diet-fed adult pigs.
29975568	1	61	theme	Perinatal	172:180	arg1	nutrition					182:190	Perinatal nutrition	172:190	Perinatal nutrition	172:190	Perinatal nutrition programs physiologic and metabolic functions, with consequences on the susceptibility to develop metabolic diseases in adulthood.
29975568	10	62	dep	microbiota	1599:1608	arg1	microbiota					1859:1868	intestinal microbiota	1848:1868	intestinal microbiota	1848:1868	Our results highlight the key role of perinatal nutrition on later microbiota and host metabolic adaptation to an unbalanced diet.-Le Bourgot, C., Ferret-Bernard, S., Apper, E., Taminiau, B., Cahu, A., Le Normand, L., Respondek, F., Le Huërou-Luron, I., Blat, S. Perinatal short-chain fructooligosaccharides program intestinal microbiota and improve enteroinsular axis function and inflammatory status in high-fat diet-fed adult pigs.
29975568	9	63	theme	perinatal	1374:1382	arg1	supplementation					1390:1404	perinatal scFOS supplementation	1374:1404	perinatal scFOS supplementation	1374:1404	In conclusion, perinatal scFOS supplementation induced long-lasting modulation of intestinal microbiota and had beneficial consequences on the host physiology in adulthood.
29975568	3	64	theme	[short-chain	426:437	arg1	supplementation					471:485	perinatal prebiotic [short-chain fructooligosaccharides (scFOS)] supplementation	406:485	perinatal prebiotic [short-chain fructooligosaccharides (scFOS)] supplementation	406:485	We investigated whether perinatal prebiotic [short-chain fructooligosaccharides (scFOS)] supplementation improved adult metabolic health in association with microbiota changes in pigs used as human model.
29975568	0	65	theme	high-fat	142:149	arg1	pigs					166:169	high-fat diet-fed adult pigs	142:169	high-fat diet-fed adult pigs	142:169	Perinatal short-chain fructooligosaccharides program intestinal microbiota and improve enteroinsular axis function and inflammatory status in high-fat diet-fed adult pigs.
29975568	9	66	theme	long-lasting	1414:1425	arg1	modulation					1427:1436	long-lasting modulation	1414:1436	long-lasting modulation of intestinal microbiota	1414:1461	In conclusion, perinatal scFOS supplementation induced long-lasting modulation of intestinal microbiota and had beneficial consequences on the host physiology in adulthood.
29975568	3	67	theme	perinatal	406:414	arg1	supplementation					471:485	perinatal prebiotic [short-chain fructooligosaccharides (scFOS)] supplementation	406:485	perinatal prebiotic [short-chain fructooligosaccharides (scFOS)] supplementation	406:485	We investigated whether perinatal prebiotic [short-chain fructooligosaccharides (scFOS)] supplementation improved adult metabolic health in association with microbiota changes in pigs used as human model.
29975568	7	68	dep	capacity	1054:1061	arg1	secrete					1066:1072	secrete	1066:1072	to secrete glucagon-like peptide-1	1063:1096	Meanwhile, scFOS animals displayed improved capacity to secrete glucagon-like peptide-1 and improved pancreas sensitivity to glucose without any changes in peripheral insulin sensitivity.
29975568	7	68	dep	capacity	1054:1061	arg1	improved					1102:1109	improved	1102:1109	improved pancreas sensitivity to glucose without any changes in peripheral insulin sensitivity	1102:1195	Meanwhile, scFOS animals displayed improved capacity to secrete glucagon-like peptide-1 and improved pancreas sensitivity to glucose without any changes in peripheral insulin sensitivity.
29975568	10	69	theme	perinatal	1570:1578	arg1	nutrition					1580:1588	perinatal nutrition	1570:1588	perinatal nutrition	1570:1588	Our results highlight the key role of perinatal nutrition on later microbiota and host metabolic adaptation to an unbalanced diet.-Le Bourgot, C., Ferret-Bernard, S., Apper, E., Taminiau, B., Cahu, A., Le Normand, L., Respondek, F., Le Huërou-Luron, I., Blat, S. Perinatal short-chain fructooligosaccharides program intestinal microbiota and improve enteroinsular axis function and inflammatory status in high-fat diet-fed adult pigs.
29975568	7	70	dep	Meanwhile	1010:1018	arg1	scFOS					1021:1025	scFOS	1021:1025	scFOS	1021:1025	Meanwhile, scFOS animals displayed improved capacity to secrete glucagon-like peptide-1 and improved pancreas sensitivity to glucose without any changes in peripheral insulin sensitivity.
29975568	0	71	theme	program	45:51	arg1	microbiota					64:73	program intestinal microbiota	45:73	program intestinal microbiota	45:73	Perinatal short-chain fructooligosaccharides program intestinal microbiota and improve enteroinsular axis function and inflammatory status in high-fat diet-fed adult pigs.
29975568	9	72	theme	microbiota	1452:1461	arg1	modulation					1427:1436	long-lasting modulation	1414:1436	long-lasting modulation of intestinal microbiota	1414:1461	In conclusion, perinatal scFOS supplementation induced long-lasting modulation of intestinal microbiota and had beneficial consequences on the host physiology in adulthood.
29975568	10	73	theme	host	1614:1617	arg1	adaptation					1629:1638	host metabolic adaptation to an unbalanced diet.-Le Bourgot, C., Ferret-Bernard, S., Apper, E., Taminiau, B., Cahu, A., Le Normand, L., Respondek, F., Le Huërou-Luron, I., Blat, S. Perinatal short-chain fructooligosaccharides	1614:1838	host metabolic adaptation to an unbalanced diet.-Le Bourgot, C., Ferret-Bernard, S., Apper, E., Taminiau, B., Cahu, A., Le Normand, L., Respondek, F., Le Huërou-Luron, I., Blat, S. Perinatal short-chain fructooligosaccharides program	1614:1846	Our results highlight the key role of perinatal nutrition on later microbiota and host metabolic adaptation to an unbalanced diet.-Le Bourgot, C., Ferret-Bernard, S., Apper, E., Taminiau, B., Cahu, A., Le Normand, L., Respondek, F., Le Huërou-Luron, I., Blat, S. Perinatal short-chain fructooligosaccharides program intestinal microbiota and improve enteroinsular axis function and inflammatory status in high-fat diet-fed adult pigs.
29975568	10	74	theme	Normand	1737:1743	arg1	fructooligosaccharides					1817:1838	an unbalanced diet.-Le Bourgot, C., Ferret-Bernard, S., Apper, E., Taminiau, B., Cahu, A., Le Normand, L., Respondek, F., Le Huërou-Luron, I., Blat, S. Perinatal short-chain fructooligosaccharides	1643:1838	an unbalanced diet.-Le Bourgot, C., Ferret-Bernard, S., Apper, E., Taminiau, B., Cahu, A., Le Normand, L., Respondek, F., Le Huërou-Luron, I., Blat, S. Perinatal short-chain fructooligosaccharides	1643:1838	Our results highlight the key role of perinatal nutrition on later microbiota and host metabolic adaptation to an unbalanced diet.-Le Bourgot, C., Ferret-Bernard, S., Apper, E., Taminiau, B., Cahu, A., Le Normand, L., Respondek, F., Le Huërou-Luron, I., Blat, S. Perinatal short-chain fructooligosaccharides program intestinal microbiota and improve enteroinsular axis function and inflammatory status in high-fat diet-fed adult pigs.
29975568	5	75	theme	high-fat	809:816	arg1	diet					818:821	a high-fat diet	807:821	a high-fat diet for 3 mo once adults	807:842	Pigs were then fed a standard diet for 5 mo, followed by a high-fat diet for 3 mo once adults.
29975568	3	76	theme	adult	496:500	arg1	health					512:517	adult metabolic health	496:517	adult metabolic health	496:517	We investigated whether perinatal prebiotic [short-chain fructooligosaccharides (scFOS)] supplementation improved adult metabolic health in association with microbiota changes in pigs used as human model.
29975568	9	77	contain	had	1467:1469	arg2	consequences					1482:1493	beneficial consequences	1471:1493	beneficial consequences	1471:1493	In conclusion, perinatal scFOS supplementation induced long-lasting modulation of intestinal microbiota and had beneficial consequences on the host physiology in adulthood.
29975568	9	77	contain	had	1467:1469	arg1	supplementation					1390:1404	perinatal scFOS supplementation	1374:1404	perinatal scFOS supplementation	1374:1404	In conclusion, perinatal scFOS supplementation induced long-lasting modulation of intestinal microbiota and had beneficial consequences on the host physiology in adulthood.
29975568	3	78	theme	microbiota	539:548	arg1	changes					550:556	microbiota changes	539:556	microbiota changes in pigs used as human model	539:584	We investigated whether perinatal prebiotic [short-chain fructooligosaccharides (scFOS)] supplementation improved adult metabolic health in association with microbiota changes in pigs used as human model.
29975568	5	79	theme	standard	771:778	arg1	diet					780:783	a standard diet	769:783	a standard diet for 5 mo	769:792	Pigs were then fed a standard diet for 5 mo, followed by a high-fat diet for 3 mo once adults.
29975568	10	80	theme	adult	1955:1959	arg1	pigs					1961:1964	high-fat diet-fed adult pigs	1937:1964	high-fat diet-fed adult pigs	1937:1964	Our results highlight the key role of perinatal nutrition on later microbiota and host metabolic adaptation to an unbalanced diet.-Le Bourgot, C., Ferret-Bernard, S., Apper, E., Taminiau, B., Cahu, A., Le Normand, L., Respondek, F., Le Huërou-Luron, I., Blat, S. Perinatal short-chain fructooligosaccharides program intestinal microbiota and improve enteroinsular axis function and inflammatory status in high-fat diet-fed adult pigs.
29975568	10	81	dep	Normand	1737:1743	arg1	Blat					1786:1789	Blat	1786:1789	Blat	1786:1789	Our results highlight the key role of perinatal nutrition on later microbiota and host metabolic adaptation to an unbalanced diet.-Le Bourgot, C., Ferret-Bernard, S., Apper, E., Taminiau, B., Cahu, A., Le Normand, L., Respondek, F., Le Huërou-Luron, I., Blat, S. Perinatal short-chain fructooligosaccharides program intestinal microbiota and improve enteroinsular axis function and inflammatory status in high-fat diet-fed adult pigs.
29975568	10	81	dep	Normand	1737:1743	arg1	Le					1765:1766	Le	1765:1766	Le	1765:1766	Our results highlight the key role of perinatal nutrition on later microbiota and host metabolic adaptation to an unbalanced diet.-Le Bourgot, C., Ferret-Bernard, S., Apper, E., Taminiau, B., Cahu, A., Le Normand, L., Respondek, F., Le Huërou-Luron, I., Blat, S. Perinatal short-chain fructooligosaccharides program intestinal microbiota and improve enteroinsular axis function and inflammatory status in high-fat diet-fed adult pigs.
29975568	10	81	dep	Normand	1737:1743	arg1	S.					1792:1793	S.	1792:1793	S.	1792:1793	Our results highlight the key role of perinatal nutrition on later microbiota and host metabolic adaptation to an unbalanced diet.-Le Bourgot, C., Ferret-Bernard, S., Apper, E., Taminiau, B., Cahu, A., Le Normand, L., Respondek, F., Le Huërou-Luron, I., Blat, S. Perinatal short-chain fructooligosaccharides program intestinal microbiota and improve enteroinsular axis function and inflammatory status in high-fat diet-fed adult pigs.
29975568	10	81	dep	Normand	1737:1743	arg1	Respondek					1750:1758	Respondek	1750:1758	Respondek	1750:1758	Our results highlight the key role of perinatal nutrition on later microbiota and host metabolic adaptation to an unbalanced diet.-Le Bourgot, C., Ferret-Bernard, S., Apper, E., Taminiau, B., Cahu, A., Le Normand, L., Respondek, F., Le Huërou-Luron, I., Blat, S. Perinatal short-chain fructooligosaccharides program intestinal microbiota and improve enteroinsular axis function and inflammatory status in high-fat diet-fed adult pigs.
29975568	10	81	dep	Normand	1737:1743	arg1	I.					1782:1783	I.	1782:1783	I.	1782:1783	Our results highlight the key role of perinatal nutrition on later microbiota and host metabolic adaptation to an unbalanced diet.-Le Bourgot, C., Ferret-Bernard, S., Apper, E., Taminiau, B., Cahu, A., Le Normand, L., Respondek, F., Le Huërou-Luron, I., Blat, S. Perinatal short-chain fructooligosaccharides program intestinal microbiota and improve enteroinsular axis function and inflammatory status in high-fat diet-fed adult pigs.
29975568	10	81	dep	Normand	1737:1743	arg1	C.					1675:1676	C.	1675:1676	C.	1675:1676	Our results highlight the key role of perinatal nutrition on later microbiota and host metabolic adaptation to an unbalanced diet.-Le Bourgot, C., Ferret-Bernard, S., Apper, E., Taminiau, B., Cahu, A., Le Normand, L., Respondek, F., Le Huërou-Luron, I., Blat, S. Perinatal short-chain fructooligosaccharides program intestinal microbiota and improve enteroinsular axis function and inflammatory status in high-fat diet-fed adult pigs.
29975568	10	81	dep	Normand	1737:1743	arg1	F.					1761:1762	F.	1761:1762	F.	1761:1762	Our results highlight the key role of perinatal nutrition on later microbiota and host metabolic adaptation to an unbalanced diet.-Le Bourgot, C., Ferret-Bernard, S., Apper, E., Taminiau, B., Cahu, A., Le Normand, L., Respondek, F., Le Huërou-Luron, I., Blat, S. Perinatal short-chain fructooligosaccharides program intestinal microbiota and improve enteroinsular axis function and inflammatory status in high-fat diet-fed adult pigs.
29975568	3	82	from	changes	550:556	arg1	pigs					561:564	pigs	561:564	pigs used as human model	561:584	We investigated whether perinatal prebiotic [short-chain fructooligosaccharides (scFOS)] supplementation improved adult metabolic health in association with microbiota changes in pigs used as human model.
29975568	3	83	theme	human	574:578	arg1	model					580:584	human model	574:584	human model	574:584	We investigated whether perinatal prebiotic [short-chain fructooligosaccharides (scFOS)] supplementation improved adult metabolic health in association with microbiota changes in pigs used as human model.
29975568	4	84	theme	lactation	671:679	arg1	end					639:641	the end	635:641	the end of gestation and the entire lactation	635:679	Sows were supplemented with scFOS or not during the end of gestation and the entire lactation, and offspring received scFOS accordingly during 1 mo after weaning.
29975568	10	85	theme	adaptation	1629:1638	arg1	program					1840:1846	host metabolic adaptation to an unbalanced diet.-Le Bourgot, C., Ferret-Bernard, S., Apper, E., Taminiau, B., Cahu, A., Le Normand, L., Respondek, F., Le Huërou-Luron, I., Blat, S. Perinatal short-chain fructooligosaccharides program	1614:1846	host metabolic adaptation to an unbalanced diet.-Le Bourgot, C., Ferret-Bernard, S., Apper, E., Taminiau, B., Cahu, A., Le Normand, L., Respondek, F., Le Huërou-Luron, I., Blat, S. Perinatal short-chain fructooligosaccharides program	1614:1846	Our results highlight the key role of perinatal nutrition on later microbiota and host metabolic adaptation to an unbalanced diet.-Le Bourgot, C., Ferret-Bernard, S., Apper, E., Taminiau, B., Cahu, A., Le Normand, L., Respondek, F., Le Huërou-Luron, I., Blat, S. Perinatal short-chain fructooligosaccharides program intestinal microbiota and improve enteroinsular axis function and inflammatory status in high-fat diet-fed adult pigs.
29975568	10	86	theme	high-fat	1937:1944	arg1	pigs					1961:1964	high-fat diet-fed adult pigs	1937:1964	high-fat diet-fed adult pigs	1937:1964	Our results highlight the key role of perinatal nutrition on later microbiota and host metabolic adaptation to an unbalanced diet.-Le Bourgot, C., Ferret-Bernard, S., Apper, E., Taminiau, B., Cahu, A., Le Normand, L., Respondek, F., Le Huërou-Luron, I., Blat, S. Perinatal short-chain fructooligosaccharides program intestinal microbiota and improve enteroinsular axis function and inflammatory status in high-fat diet-fed adult pigs.
29975568	2	87	theme	programming	369:379	arg1	factor					354:359	a key factor	348:359	a key factor of such programming	348:379	The microbiota represents a key factor of such programming.
29975568	8	88	theme	Perinatal	1198:1206	arg1	supplementation					1214:1228	Perinatal scFOS supplementation	1198:1228	Perinatal scFOS supplementation	1198:1228	Perinatal scFOS supplementation also increased ileal secretory IgA secretion and alkaline phosphatase activity and decreased TNF-α expression in adipose tissue.
29975568	6	89	theme	Perinatal	845:853	arg1	supplementation					861:875	Perinatal scFOS supplementation	845:875	Perinatal scFOS supplementation	845:875	Perinatal scFOS supplementation induced a persistent modulation of the composition of the fecal microbiota in adulthood, notably by increasing the Prevotella genus.
29975568	1	90	theme	metabolic	289:297	arg1	diseases					299:306	metabolic diseases	289:306	metabolic diseases in adulthood	289:319	Perinatal nutrition programs physiologic and metabolic functions, with consequences on the susceptibility to develop metabolic diseases in adulthood.
30193408	9	0	from	level	1690:1694	arg1	enantiomer					1714:1723	the active (-) enantiomer	1699:1723	the active (-) enantiomer	1699:1723	In addition, the proposed method was applied in checking the enantiomeric purity of (-) BET in the presence of (+) BET at 1% level where the inactive (+) enantiomer was quantified with good accuracy and precision at 1% level in the active (-) enantiomer.
30193408	1	1	theme	betaxolol	261:269	arg1	method					247:252	Stereospecific separation method	221:252	Stereospecific separation method of (±) betaxolol, (±) carvedilol, and (±) sotalol using High Performance Thin Layer Chromatography (HPTLC) and β-cyclodextrin as chiral selector	221:397	Stereospecific separation method of (±) betaxolol, (±) carvedilol, and (±) sotalol using High Performance Thin Layer Chromatography (HPTLC) and β-cyclodextrin as chiral selector has been developed and validated.
30193408	0	2	dep	using	98:102	arg1	Application					162:172	Application	162:172	Application to check the enantiomeric purity of betaxolol	162:218	Box-Behnken response surface modeling assisted enantiomeric resolution of some racemic β-blockers using HPTLC and β-cyclodextrin as chiral mobile phase additive: Application to check the enantiomeric purity of betaxolol.
30193408	3	3	theme	mobile	719:724	arg1	acid-water					682:691	acetonitrile-methanol-acetic acid-water	653:691	acetonitrile-methanol-acetic acid-water (3.4:3.6:0.18:1 v/v)	653:712	The optimized method involves separation on Fluka HPTLC silica gel plates 60 F254 (20 × 10 cm) using acetonitrile-methanol-acetic acid-water (3.4:3.6:0.18:1 v/v) as a mobile phase containing 0.57 mM β-cyclodextrin.
30193408	3	3	theme	mobile	719:724	arg1	phase					726:730	a mobile phase	717:730	a mobile phase containing 0.57 mM β-cyclodextrin	717:764	The optimized method involves separation on Fluka HPTLC silica gel plates 60 F254 (20 × 10 cm) using acetonitrile-methanol-acetic acid-water (3.4:3.6:0.18:1 v/v) as a mobile phase containing 0.57 mM β-cyclodextrin.
30193408	6	4	theme	various	1227:1233	arg1	pharmaceuticals					1235:1249	various pharmaceuticals	1227:1249	various pharmaceuticals	1227:1249	The proposed method enables estimation of (-) and (+) enantiomers of betaxolol in drug substance and in various pharmaceuticals.
30193408	9	5	dep	BET	1559:1561	arg1	-					1556:1556	-	1556:1556	-	1556:1556	In addition, the proposed method was applied in checking the enantiomeric purity of (-) BET in the presence of (+) BET at 1% level where the inactive (+) enantiomer was quantified with good accuracy and precision at 1% level in the active (-) enantiomer.
30193408	7	6	dep	enantiomers	1329:1339	arg1	+					1326:1326	+	1326:1326	+	1326:1326	The detection limit of betaxolol was 0.15 and 0.13 μg band-1 for (-) and (+) enantiomers, respectively.
30193408	0	7	theme	chiral	132:137	arg1	phase					146:150	chiral mobile phase additive	132:159	chiral mobile phase additive	132:159	Box-Behnken response surface modeling assisted enantiomeric resolution of some racemic β-blockers using HPTLC and β-cyclodextrin as chiral mobile phase additive: Application to check the enantiomeric purity of betaxolol.
30193408	5	8	theme	phase	1080:1084	arg1	part					1061:1064	the organic part	1049:1064	the organic part of the mobile phase	1049:1084	Maximum separation of the enantiomers of the three drugs was obtained by optimizing concentration of chiral selector, the mobile phase composition including acetonitrile amount in the organic part of the mobile phase and the volume of acetic acid added.
30193408	5	9	from	amount	1039:1044	arg1	part					1061:1064	the organic part	1049:1064	the organic part of the mobile phase	1049:1084	Maximum separation of the enantiomers of the three drugs was obtained by optimizing concentration of chiral selector, the mobile phase composition including acetonitrile amount in the organic part of the mobile phase and the volume of acetic acid added.
30193408	1	10	theme	separation	236:245	arg1	method					247:252	Stereospecific separation method	221:252	Stereospecific separation method of (±) betaxolol, (±) carvedilol, and (±) sotalol using High Performance Thin Layer Chromatography (HPTLC) and β-cyclodextrin as chiral selector	221:397	Stereospecific separation method of (±) betaxolol, (±) carvedilol, and (±) sotalol using High Performance Thin Layer Chromatography (HPTLC) and β-cyclodextrin as chiral selector has been developed and validated.
30193408	9	11	theme	%	1594:1594	arg1	level					1596:1600	1% level	1593:1600	1% level	1593:1600	In addition, the proposed method was applied in checking the enantiomeric purity of (-) BET in the presence of (+) BET at 1% level where the inactive (+) enantiomer was quantified with good accuracy and precision at 1% level in the active (-) enantiomer.
30193408	3	12	theme	silica	608:613	arg1	plates					619:624	Fluka HPTLC silica gel plates 60 F254 (20 × 10 cm)	596:645	Fluka HPTLC silica gel plates 60 F254 (20 × 10 cm) using acetonitrile-methanol-acetic acid-water (3.4:3.6:0.18:1 v/v) as a mobile phase containing 0.57 mM β-cyclodextrin	596:764	The optimized method involves separation on Fluka HPTLC silica gel plates 60 F254 (20 × 10 cm) using acetonitrile-methanol-acetic acid-water (3.4:3.6:0.18:1 v/v) as a mobile phase containing 0.57 mM β-cyclodextrin.
30193408	9	13	theme	inactive	1612:1619	arg1	enantiomer					1625:1634	the inactive (+) enantiomer	1608:1634	the inactive (+) enantiomer	1608:1634	In addition, the proposed method was applied in checking the enantiomeric purity of (-) BET in the presence of (+) BET at 1% level where the inactive (+) enantiomer was quantified with good accuracy and precision at 1% level in the active (-) enantiomer.
30193408	1	14	theme	sotalol	296:302	arg1	method					247:252	Stereospecific separation method	221:252	Stereospecific separation method of (±) betaxolol, (±) carvedilol, and (±) sotalol using High Performance Thin Layer Chromatography (HPTLC) and β-cyclodextrin as chiral selector	221:397	Stereospecific separation method of (±) betaxolol, (±) carvedilol, and (±) sotalol using High Performance Thin Layer Chromatography (HPTLC) and β-cyclodextrin as chiral selector has been developed and validated.
30193408	3	15	theme	Fluka	596:600	arg1	plates					619:624	Fluka HPTLC silica gel plates 60 F254 (20 × 10 cm)	596:645	Fluka HPTLC silica gel plates 60 F254 (20 × 10 cm) using acetonitrile-methanol-acetic acid-water (3.4:3.6:0.18:1 v/v) as a mobile phase containing 0.57 mM β-cyclodextrin	596:764	The optimized method involves separation on Fluka HPTLC silica gel plates 60 F254 (20 × 10 cm) using acetonitrile-methanol-acetic acid-water (3.4:3.6:0.18:1 v/v) as a mobile phase containing 0.57 mM β-cyclodextrin.
30193408	9	16	from	level	1596:1600	arg1	presence					1570:1577	the presence	1566:1577	the presence of (+) BET at 1% level	1566:1600	In addition, the proposed method was applied in checking the enantiomeric purity of (-) BET in the presence of (+) BET at 1% level where the inactive (+) enantiomer was quantified with good accuracy and precision at 1% level in the active (-) enantiomer.
30193408	0	17	theme	β-blockers	87:96	arg1	resolution					60:69	Box-Behnken response surface modeling assisted enantiomeric resolution	0:69	Box-Behnken response surface modeling assisted enantiomeric resolution of some racemic β-blockers	0:96	Box-Behnken response surface modeling assisted enantiomeric resolution of some racemic β-blockers using HPTLC and β-cyclodextrin as chiral mobile phase additive: Application to check the enantiomeric purity of betaxolol.
30193408	1	18	theme	High	310:313	arg1	HPTLC					354:358	HPTLC	354:358	HPTLC	354:358	Stereospecific separation method of (±) betaxolol, (±) carvedilol, and (±) sotalol using High Performance Thin Layer Chromatography (HPTLC) and β-cyclodextrin as chiral selector has been developed and validated.
30193408	1	18	theme	High	310:313	arg1	Chromatography					338:351	High Performance Thin Layer Chromatography	310:351	High Performance Thin Layer Chromatography (HPTLC)	310:359	Stereospecific separation method of (±) betaxolol, (±) carvedilol, and (±) sotalol using High Performance Thin Layer Chromatography (HPTLC) and β-cyclodextrin as chiral selector has been developed and validated.
30193408	2	19	theme	operating	505:513	arg1	variables					515:523	the operating variables	501:523	the operating variables based on 15 trials design	501:549	The Box-Behnken surface response design was selected for optimizing the operating variables based on 15 trials design.
30193408	1	20	theme	Thin	327:330	arg1	HPTLC					354:358	HPTLC	354:358	HPTLC	354:358	Stereospecific separation method of (±) betaxolol, (±) carvedilol, and (±) sotalol using High Performance Thin Layer Chromatography (HPTLC) and β-cyclodextrin as chiral selector has been developed and validated.
30193408	1	20	theme	Thin	327:330	arg1	Chromatography					338:351	High Performance Thin Layer Chromatography	310:351	High Performance Thin Layer Chromatography (HPTLC)	310:359	Stereospecific separation method of (±) betaxolol, (±) carvedilol, and (±) sotalol using High Performance Thin Layer Chromatography (HPTLC) and β-cyclodextrin as chiral selector has been developed and validated.
30193408	5	21	theme	acid	1111:1114	arg1	volume					1094:1099	the volume	1090:1099	the volume of acetic acid added	1090:1120	Maximum separation of the enantiomers of the three drugs was obtained by optimizing concentration of chiral selector, the mobile phase composition including acetonitrile amount in the organic part of the mobile phase and the volume of acetic acid added.
30193408	5	21	theme	acid	1111:1114	arg1	concentration					953:965	concentration	953:965	concentration of chiral selector	953:984	Maximum separation of the enantiomers of the three drugs was obtained by optimizing concentration of chiral selector, the mobile phase composition including acetonitrile amount in the organic part of the mobile phase and the volume of acetic acid added.
30193408	5	21	theme	acid	1111:1114	arg1	composition					1004:1014	the mobile phase composition	987:1014	the mobile phase composition including acetonitrile amount in the organic part of the mobile phase	987:1084	Maximum separation of the enantiomers of the three drugs was obtained by optimizing concentration of chiral selector, the mobile phase composition including acetonitrile amount in the organic part of the mobile phase and the volume of acetic acid added.
30193408	9	22	dep	enantiomer	1714:1723	arg1	-					1711:1711	-	1711:1711	-	1711:1711	In addition, the proposed method was applied in checking the enantiomeric purity of (-) BET in the presence of (+) BET at 1% level where the inactive (+) enantiomer was quantified with good accuracy and precision at 1% level in the active (-) enantiomer.
30193408	5	23	theme	mobile	991:996	arg1	composition					1004:1014	the mobile phase composition	987:1014	the mobile phase composition including acetonitrile amount in the organic part of the mobile phase	987:1084	Maximum separation of the enantiomers of the three drugs was obtained by optimizing concentration of chiral selector, the mobile phase composition including acetonitrile amount in the organic part of the mobile phase and the volume of acetic acid added.
30193408	9	24	theme	good	1656:1659	arg1	accuracy					1661:1668	good accuracy	1656:1668	good accuracy	1656:1668	In addition, the proposed method was applied in checking the enantiomeric purity of (-) BET in the presence of (+) BET at 1% level where the inactive (+) enantiomer was quantified with good accuracy and precision at 1% level in the active (-) enantiomer.
30193408	0	25	theme	Box-Behnken	0:10	arg1	resolution					60:69	Box-Behnken response surface modeling assisted enantiomeric resolution	0:69	Box-Behnken response surface modeling assisted enantiomeric resolution of some racemic β-blockers	0:96	Box-Behnken response surface modeling assisted enantiomeric resolution of some racemic β-blockers using HPTLC and β-cyclodextrin as chiral mobile phase additive: Application to check the enantiomeric purity of betaxolol.
30193408	1	26	theme	±	273:273	arg1	carvedilol					276:285	(±) carvedilol	272:285	(±) carvedilol	272:285	Stereospecific separation method of (±) betaxolol, (±) carvedilol, and (±) sotalol using High Performance Thin Layer Chromatography (HPTLC) and β-cyclodextrin as chiral selector has been developed and validated.
30193408	0	27	theme	surface	21:27	arg1	resolution					60:69	Box-Behnken response surface modeling assisted enantiomeric resolution	0:69	Box-Behnken response surface modeling assisted enantiomeric resolution of some racemic β-blockers	0:96	Box-Behnken response surface modeling assisted enantiomeric resolution of some racemic β-blockers using HPTLC and β-cyclodextrin as chiral mobile phase additive: Application to check the enantiomeric purity of betaxolol.
30193408	1	28	theme	carvedilol	276:285	arg1	method					247:252	Stereospecific separation method	221:252	Stereospecific separation method of (±) betaxolol, (±) carvedilol, and (±) sotalol using High Performance Thin Layer Chromatography (HPTLC) and β-cyclodextrin as chiral selector	221:397	Stereospecific separation method of (±) betaxolol, (±) carvedilol, and (±) sotalol using High Performance Thin Layer Chromatography (HPTLC) and β-cyclodextrin as chiral selector has been developed and validated.
30193408	5	29	theme	chiral	970:975	arg1	selector					977:984	chiral selector	970:984	chiral selector	970:984	Maximum separation of the enantiomers of the three drugs was obtained by optimizing concentration of chiral selector, the mobile phase composition including acetonitrile amount in the organic part of the mobile phase and the volume of acetic acid added.
30193408	2	30	theme	surface	449:455	arg1	design					466:471	The Box-Behnken surface response design	433:471	The Box-Behnken surface response design	433:471	The Box-Behnken surface response design was selected for optimizing the operating variables based on 15 trials design.
30193408	3	31	from	separation	582:591	arg1	plates					619:624	Fluka HPTLC silica gel plates 60 F254 (20 × 10 cm)	596:645	Fluka HPTLC silica gel plates 60 F254 (20 × 10 cm) using acetonitrile-methanol-acetic acid-water (3.4:3.6:0.18:1 v/v) as a mobile phase containing 0.57 mM β-cyclodextrin	596:764	The optimized method involves separation on Fluka HPTLC silica gel plates 60 F254 (20 × 10 cm) using acetonitrile-methanol-acetic acid-water (3.4:3.6:0.18:1 v/v) as a mobile phase containing 0.57 mM β-cyclodextrin.
30193408	0	32	theme	assisted	38:45	arg1	resolution					60:69	Box-Behnken response surface modeling assisted enantiomeric resolution	0:69	Box-Behnken response surface modeling assisted enantiomeric resolution of some racemic β-blockers	0:96	Box-Behnken response surface modeling assisted enantiomeric resolution of some racemic β-blockers using HPTLC and β-cyclodextrin as chiral mobile phase additive: Application to check the enantiomeric purity of betaxolol.
30193408	2	33	theme	Box-Behnken	437:447	arg1	design					466:471	The Box-Behnken surface response design	433:471	The Box-Behnken surface response design	433:471	The Box-Behnken surface response design was selected for optimizing the operating variables based on 15 trials design.
30193408	7	34	theme	betaxolol	1275:1283	arg1	band-1					1306:1311	0.15 and 0.13 μg band-1	1289:1311	0.15 and 0.13 μg band-1 for (-)	1289:1319	The detection limit of betaxolol was 0.15 and 0.13 μg band-1 for (-) and (+) enantiomers, respectively.
30193408	7	34	theme	betaxolol	1275:1283	arg1	limit					1266:1270	The detection limit	1252:1270	The detection limit of betaxolol	1252:1283	The detection limit of betaxolol was 0.15 and 0.13 μg band-1 for (-) and (+) enantiomers, respectively.
30193408	9	35	theme	%	1688:1688	arg1	level					1690:1694	1% level	1687:1694	1% level in the active (-) enantiomer	1687:1723	In addition, the proposed method was applied in checking the enantiomeric purity of (-) BET in the presence of (+) BET at 1% level where the inactive (+) enantiomer was quantified with good accuracy and precision at 1% level in the active (-) enantiomer.
30193408	1	36	theme	±	293:293	arg1	sotalol					296:302	(±) sotalol	292:302	(±) sotalol	292:302	Stereospecific separation method of (±) betaxolol, (±) carvedilol, and (±) sotalol using High Performance Thin Layer Chromatography (HPTLC) and β-cyclodextrin as chiral selector has been developed and validated.
30193408	0	37	theme	betaxolol	210:218	arg1	purity					200:205	the enantiomeric purity	183:205	the enantiomeric purity of betaxolol	183:218	Box-Behnken response surface modeling assisted enantiomeric resolution of some racemic β-blockers using HPTLC and β-cyclodextrin as chiral mobile phase additive: Application to check the enantiomeric purity of betaxolol.
30193408	6	38	theme	proposed	1127:1134	arg1	method					1136:1141	The proposed method	1123:1141	The proposed method	1123:1141	The proposed method enables estimation of (-) and (+) enantiomers of betaxolol in drug substance and in various pharmaceuticals.
30193408	3	39	theme	acetonitrile-methanol-acetic	653:680	arg1	3.4:3.6:0.18:1 v/v					694:711	3.4:3.6:0.18:1 v/v	694:711	3.4:3.6:0.18:1 v/v	694:711	The optimized method involves separation on Fluka HPTLC silica gel plates 60 F254 (20 × 10 cm) using acetonitrile-methanol-acetic acid-water (3.4:3.6:0.18:1 v/v) as a mobile phase containing 0.57 mM β-cyclodextrin.
30193408	3	39	theme	acetonitrile-methanol-acetic	653:680	arg1	acid-water					682:691	acetonitrile-methanol-acetic acid-water	653:691	acetonitrile-methanol-acetic acid-water (3.4:3.6:0.18:1 v/v)	653:712	The optimized method involves separation on Fluka HPTLC silica gel plates 60 F254 (20 × 10 cm) using acetonitrile-methanol-acetic acid-water (3.4:3.6:0.18:1 v/v) as a mobile phase containing 0.57 mM β-cyclodextrin.
30193408	3	39	theme	acetonitrile-methanol-acetic	653:680	arg1	phase					726:730	a mobile phase	717:730	a mobile phase containing 0.57 mM β-cyclodextrin	717:764	The optimized method involves separation on Fluka HPTLC silica gel plates 60 F254 (20 × 10 cm) using acetonitrile-methanol-acetic acid-water (3.4:3.6:0.18:1 v/v) as a mobile phase containing 0.57 mM β-cyclodextrin.
30193408	9	40	theme	active	1703:1708	arg1	enantiomer					1714:1723	the active (-) enantiomer	1699:1723	the active (-) enantiomer	1699:1723	In addition, the proposed method was applied in checking the enantiomeric purity of (-) BET in the presence of (+) BET at 1% level where the inactive (+) enantiomer was quantified with good accuracy and precision at 1% level in the active (-) enantiomer.
30193408	5	41	theme	acetonitrile	1026:1037	arg1	amount					1039:1044	acetonitrile amount	1026:1044	acetonitrile amount in the organic part of the mobile phase	1026:1084	Maximum separation of the enantiomers of the three drugs was obtained by optimizing concentration of chiral selector, the mobile phase composition including acetonitrile amount in the organic part of the mobile phase and the volume of acetic acid added.
30193408	0	42	theme	enantiomeric	187:198	arg1	purity					200:205	the enantiomeric purity	183:205	the enantiomeric purity of betaxolol	183:218	Box-Behnken response surface modeling assisted enantiomeric resolution of some racemic β-blockers using HPTLC and β-cyclodextrin as chiral mobile phase additive: Application to check the enantiomeric purity of betaxolol.
30193408	9	43	theme	BET	1559:1561	arg1	checking					1519:1526	checking	1519:1526	checking the enantiomeric purity of (-) BET	1519:1561	In addition, the proposed method was applied in checking the enantiomeric purity of (-) BET in the presence of (+) BET at 1% level where the inactive (+) enantiomer was quantified with good accuracy and precision at 1% level in the active (-) enantiomer.
30193408	6	44	theme	and	1169:1171	arg1	enantiomers					1177:1187	(-) and (+) enantiomers	1165:1187	(-) and (+) enantiomers of betaxolol	1165:1200	The proposed method enables estimation of (-) and (+) enantiomers of betaxolol in drug substance and in various pharmaceuticals.
30193408	5	45	theme	Maximum	869:875	arg1	separation					877:886	Maximum separation	869:886	Maximum separation of the enantiomers of the three drugs	869:924	Maximum separation of the enantiomers of the three drugs was obtained by optimizing concentration of chiral selector, the mobile phase composition including acetonitrile amount in the organic part of the mobile phase and the volume of acetic acid added.
30193408	9	46	dep	BET	1586:1588	arg1	+					1583:1583	+	1583:1583	+	1583:1583	In addition, the proposed method was applied in checking the enantiomeric purity of (-) BET in the presence of (+) BET at 1% level where the inactive (+) enantiomer was quantified with good accuracy and precision at 1% level in the active (-) enantiomer.
30193408	9	47	dep	checking	1519:1526	arg1	purity					1545:1550	the enantiomeric purity	1528:1550	checking the enantiomeric purity of (-) BET	1519:1561	In addition, the proposed method was applied in checking the enantiomeric purity of (-) BET in the presence of (+) BET at 1% level where the inactive (+) enantiomer was quantified with good accuracy and precision at 1% level in the active (-) enantiomer.
30193408	0	48	theme	mobile	139:144	arg1	phase					146:150	chiral mobile phase additive	132:159	chiral mobile phase additive	132:159	Box-Behnken response surface modeling assisted enantiomeric resolution of some racemic β-blockers using HPTLC and β-cyclodextrin as chiral mobile phase additive: Application to check the enantiomeric purity of betaxolol.
30193408	9	49	theme	enantiomeric	1532:1543	arg1	purity					1545:1550	the enantiomeric purity	1528:1550	checking the enantiomeric purity of (-) BET	1519:1561	In addition, the proposed method was applied in checking the enantiomeric purity of (-) BET in the presence of (+) BET at 1% level where the inactive (+) enantiomer was quantified with good accuracy and precision at 1% level in the active (-) enantiomer.
30193408	9	50	from	presence	1570:1577	arg1	level					1596:1600	1% level	1593:1600	1% level	1593:1600	In addition, the proposed method was applied in checking the enantiomeric purity of (-) BET in the presence of (+) BET at 1% level where the inactive (+) enantiomer was quantified with good accuracy and precision at 1% level in the active (-) enantiomer.
30193408	9	51	theme	BET	1586:1588	arg1	presence					1570:1577	the presence	1566:1577	the presence of (+) BET at 1% level	1566:1600	In addition, the proposed method was applied in checking the enantiomeric purity of (-) BET in the presence of (+) BET at 1% level where the inactive (+) enantiomer was quantified with good accuracy and precision at 1% level in the active (-) enantiomer.
30193408	3	52	theme	0.57 mM	743:749	arg1	β-cyclodextrin					751:764	0.57 mM β-cyclodextrin	743:764	0.57 mM β-cyclodextrin	743:764	The optimized method involves separation on Fluka HPTLC silica gel plates 60 F254 (20 × 10 cm) using acetonitrile-methanol-acetic acid-water (3.4:3.6:0.18:1 v/v) as a mobile phase containing 0.57 mM β-cyclodextrin.
30193408	0	53	theme	additive	152:159	arg1	phase					146:150	chiral mobile phase additive	132:159	chiral mobile phase additive	132:159	Box-Behnken response surface modeling assisted enantiomeric resolution of some racemic β-blockers using HPTLC and β-cyclodextrin as chiral mobile phase additive: Application to check the enantiomeric purity of betaxolol.
30193408	1	54	theme	Stereospecific	221:234	arg1	method					247:252	Stereospecific separation method	221:252	Stereospecific separation method of (±) betaxolol, (±) carvedilol, and (±) sotalol using High Performance Thin Layer Chromatography (HPTLC) and β-cyclodextrin as chiral selector	221:397	Stereospecific separation method of (±) betaxolol, (±) carvedilol, and (±) sotalol using High Performance Thin Layer Chromatography (HPTLC) and β-cyclodextrin as chiral selector has been developed and validated.
30193408	9	55	theme	1	1593:1593	arg1	%					1594:1594	%	1594:1594	%	1594:1594	In addition, the proposed method was applied in checking the enantiomeric purity of (-) BET in the presence of (+) BET at 1% level where the inactive (+) enantiomer was quantified with good accuracy and precision at 1% level in the active (-) enantiomer.
30193408	6	56	theme	drug	1205:1208	arg1	substance					1210:1218	drug substance	1205:1218	drug substance	1205:1218	The proposed method enables estimation of (-) and (+) enantiomers of betaxolol in drug substance and in various pharmaceuticals.
30193408	3	57	theme	optimized	556:564	arg1	method					566:571	The optimized method	552:571	The optimized method	552:571	The optimized method involves separation on Fluka HPTLC silica gel plates 60 F254 (20 × 10 cm) using acetonitrile-methanol-acetic acid-water (3.4:3.6:0.18:1 v/v) as a mobile phase containing 0.57 mM β-cyclodextrin.
30193408	5	58	theme	drugs	920:924	arg1	enantiomers					895:905	the enantiomers	891:905	the enantiomers of the three drugs	891:924	Maximum separation of the enantiomers of the three drugs was obtained by optimizing concentration of chiral selector, the mobile phase composition including acetonitrile amount in the organic part of the mobile phase and the volume of acetic acid added.
30193408	6	59	theme	betaxolol	1192:1200	arg1	enantiomers					1177:1187	(-) and (+) enantiomers	1165:1187	(-) and (+) enantiomers of betaxolol	1165:1200	The proposed method enables estimation of (-) and (+) enantiomers of betaxolol in drug substance and in various pharmaceuticals.
30193408	9	60	dep	enantiomer	1625:1634	arg1	+					1622:1622	+	1622:1622	+	1622:1622	In addition, the proposed method was applied in checking the enantiomeric purity of (-) BET in the presence of (+) BET at 1% level where the inactive (+) enantiomer was quantified with good accuracy and precision at 1% level in the active (-) enantiomer.
30193408	1	61	theme	chiral	383:388	arg1	selector					390:397	chiral selector	383:397	chiral selector	383:397	Stereospecific separation method of (±) betaxolol, (±) carvedilol, and (±) sotalol using High Performance Thin Layer Chromatography (HPTLC) and β-cyclodextrin as chiral selector has been developed and validated.
30193408	3	62	theme	HPTLC	602:606	arg1	plates					619:624	Fluka HPTLC silica gel plates 60 F254 (20 × 10 cm)	596:645	Fluka HPTLC silica gel plates 60 F254 (20 × 10 cm) using acetonitrile-methanol-acetic acid-water (3.4:3.6:0.18:1 v/v) as a mobile phase containing 0.57 mM β-cyclodextrin	596:764	The optimized method involves separation on Fluka HPTLC silica gel plates 60 F254 (20 × 10 cm) using acetonitrile-methanol-acetic acid-water (3.4:3.6:0.18:1 v/v) as a mobile phase containing 0.57 mM β-cyclodextrin.
30193408	2	63	theme	trials	537:542	arg1	design					544:549	15 trials design	534:549	15 trials design	534:549	The Box-Behnken surface response design was selected for optimizing the operating variables based on 15 trials design.
30193408	6	64	theme	enantiomers	1177:1187	arg1	estimation					1151:1160	estimation	1151:1160	estimation of (-) and (+) enantiomers of betaxolol in drug substance and in various pharmaceuticals	1151:1249	The proposed method enables estimation of (-) and (+) enantiomers of betaxolol in drug substance and in various pharmaceuticals.
30193408	6	65	dep	enantiomers	1177:1187	arg1	-					1166:1166	-	1166:1166	-	1166:1166	The proposed method enables estimation of (-) and (+) enantiomers of betaxolol in drug substance and in various pharmaceuticals.
30193408	0	66	theme	racemic	79:85	arg1	β-blockers					87:96	some racemic β-blockers	74:96	some racemic β-blockers	74:96	Box-Behnken response surface modeling assisted enantiomeric resolution of some racemic β-blockers using HPTLC and β-cyclodextrin as chiral mobile phase additive: Application to check the enantiomeric purity of betaxolol.
30193408	5	67	theme	mobile	1073:1078	arg1	phase					1080:1084	the mobile phase	1069:1084	the mobile phase	1069:1084	Maximum separation of the enantiomers of the three drugs was obtained by optimizing concentration of chiral selector, the mobile phase composition including acetonitrile amount in the organic part of the mobile phase and the volume of acetic acid added.
30193408	5	68	theme	enantiomers	895:905	arg1	separation					877:886	Maximum separation	869:886	Maximum separation of the enantiomers of the three drugs	869:924	Maximum separation of the enantiomers of the three drugs was obtained by optimizing concentration of chiral selector, the mobile phase composition including acetonitrile amount in the organic part of the mobile phase and the volume of acetic acid added.
30193408	2	69	theme	response	457:464	arg1	design					466:471	The Box-Behnken surface response design	433:471	The Box-Behnken surface response design	433:471	The Box-Behnken surface response design was selected for optimizing the operating variables based on 15 trials design.
30193408	1	70	theme	Performance	315:325	arg1	HPTLC					354:358	HPTLC	354:358	HPTLC	354:358	Stereospecific separation method of (±) betaxolol, (±) carvedilol, and (±) sotalol using High Performance Thin Layer Chromatography (HPTLC) and β-cyclodextrin as chiral selector has been developed and validated.
30193408	1	70	theme	Performance	315:325	arg1	Chromatography					338:351	High Performance Thin Layer Chromatography	310:351	High Performance Thin Layer Chromatography (HPTLC)	310:359	Stereospecific separation method of (±) betaxolol, (±) carvedilol, and (±) sotalol using High Performance Thin Layer Chromatography (HPTLC) and β-cyclodextrin as chiral selector has been developed and validated.
30193408	5	71	theme	selector	977:984	arg1	volume					1094:1099	the volume	1090:1099	the volume of acetic acid added	1090:1120	Maximum separation of the enantiomers of the three drugs was obtained by optimizing concentration of chiral selector, the mobile phase composition including acetonitrile amount in the organic part of the mobile phase and the volume of acetic acid added.
30193408	5	71	theme	selector	977:984	arg1	concentration					953:965	concentration	953:965	concentration of chiral selector	953:984	Maximum separation of the enantiomers of the three drugs was obtained by optimizing concentration of chiral selector, the mobile phase composition including acetonitrile amount in the organic part of the mobile phase and the volume of acetic acid added.
30193408	5	71	theme	selector	977:984	arg1	composition					1004:1014	the mobile phase composition	987:1014	the mobile phase composition including acetonitrile amount in the organic part of the mobile phase	987:1084	Maximum separation of the enantiomers of the three drugs was obtained by optimizing concentration of chiral selector, the mobile phase composition including acetonitrile amount in the organic part of the mobile phase and the volume of acetic acid added.
30193408	4	72	theme	Densitometric	767:779	arg1	measurements					781:792	Densitometric measurements	767:792	Densitometric measurements	767:792	Densitometric measurements were made at 220 nm for betaxolol and sotalol or at 245 nm for carvedilol.
30193408	1	73	theme	Layer	332:336	arg1	HPTLC					354:358	HPTLC	354:358	HPTLC	354:358	Stereospecific separation method of (±) betaxolol, (±) carvedilol, and (±) sotalol using High Performance Thin Layer Chromatography (HPTLC) and β-cyclodextrin as chiral selector has been developed and validated.
30193408	1	73	theme	Layer	332:336	arg1	Chromatography					338:351	High Performance Thin Layer Chromatography	310:351	High Performance Thin Layer Chromatography (HPTLC)	310:359	Stereospecific separation method of (±) betaxolol, (±) carvedilol, and (±) sotalol using High Performance Thin Layer Chromatography (HPTLC) and β-cyclodextrin as chiral selector has been developed and validated.
30193408	0	74	theme	response	12:19	arg1	resolution					60:69	Box-Behnken response surface modeling assisted enantiomeric resolution	0:69	Box-Behnken response surface modeling assisted enantiomeric resolution of some racemic β-blockers	0:96	Box-Behnken response surface modeling assisted enantiomeric resolution of some racemic β-blockers using HPTLC and β-cyclodextrin as chiral mobile phase additive: Application to check the enantiomeric purity of betaxolol.
30193408	9	75	theme	proposed	1488:1495	arg1	method					1497:1502	the proposed method	1484:1502	the proposed method	1484:1502	In addition, the proposed method was applied in checking the enantiomeric purity of (-) BET in the presence of (+) BET at 1% level where the inactive (+) enantiomer was quantified with good accuracy and precision at 1% level in the active (-) enantiomer.
30193408	5	76	theme	acetic	1104:1109	arg1	acid					1111:1114	acetic acid	1104:1114	acetic acid added	1104:1120	Maximum separation of the enantiomers of the three drugs was obtained by optimizing concentration of chiral selector, the mobile phase composition including acetonitrile amount in the organic part of the mobile phase and the volume of acetic acid added.
30193408	3	77	contain	containing	732:741	arg1	acid-water					682:691	acetonitrile-methanol-acetic acid-water	653:691	acetonitrile-methanol-acetic acid-water (3.4:3.6:0.18:1 v/v)	653:712	The optimized method involves separation on Fluka HPTLC silica gel plates 60 F254 (20 × 10 cm) using acetonitrile-methanol-acetic acid-water (3.4:3.6:0.18:1 v/v) as a mobile phase containing 0.57 mM β-cyclodextrin.
30193408	3	77	contain	containing	732:741	arg2	β-cyclodextrin					751:764	0.57 mM β-cyclodextrin	743:764	0.57 mM β-cyclodextrin	743:764	The optimized method involves separation on Fluka HPTLC silica gel plates 60 F254 (20 × 10 cm) using acetonitrile-methanol-acetic acid-water (3.4:3.6:0.18:1 v/v) as a mobile phase containing 0.57 mM β-cyclodextrin.
30193408	3	77	contain	containing	732:741	arg1	phase					726:730	a mobile phase	717:730	a mobile phase containing 0.57 mM β-cyclodextrin	717:764	The optimized method involves separation on Fluka HPTLC silica gel plates 60 F254 (20 × 10 cm) using acetonitrile-methanol-acetic acid-water (3.4:3.6:0.18:1 v/v) as a mobile phase containing 0.57 mM β-cyclodextrin.
30193408	6	78	from	estimation	1151:1160	arg1	pharmaceuticals					1235:1249	various pharmaceuticals	1227:1249	various pharmaceuticals	1227:1249	The proposed method enables estimation of (-) and (+) enantiomers of betaxolol in drug substance and in various pharmaceuticals.
30193408	6	78	from	estimation	1151:1160	arg1	substance					1210:1218	drug substance	1205:1218	drug substance	1205:1218	The proposed method enables estimation of (-) and (+) enantiomers of betaxolol in drug substance and in various pharmaceuticals.
30193408	0	79	theme	modeling	29:36	arg1	resolution					60:69	Box-Behnken response surface modeling assisted enantiomeric resolution	0:69	Box-Behnken response surface modeling assisted enantiomeric resolution of some racemic β-blockers	0:96	Box-Behnken response surface modeling assisted enantiomeric resolution of some racemic β-blockers using HPTLC and β-cyclodextrin as chiral mobile phase additive: Application to check the enantiomeric purity of betaxolol.
30193408	0	80	theme	enantiomeric	47:58	arg1	resolution					60:69	Box-Behnken response surface modeling assisted enantiomeric resolution	0:69	Box-Behnken response surface modeling assisted enantiomeric resolution of some racemic β-blockers	0:96	Box-Behnken response surface modeling assisted enantiomeric resolution of some racemic β-blockers using HPTLC and β-cyclodextrin as chiral mobile phase additive: Application to check the enantiomeric purity of betaxolol.
30193408	9	81	theme	1	1687:1687	arg1	%					1688:1688	%	1688:1688	%	1688:1688	In addition, the proposed method was applied in checking the enantiomeric purity of (-) BET in the presence of (+) BET at 1% level where the inactive (+) enantiomer was quantified with good accuracy and precision at 1% level in the active (-) enantiomer.
30193408	3	82	theme	gel	615:617	arg1	plates					619:624	Fluka HPTLC silica gel plates 60 F254 (20 × 10 cm)	596:645	Fluka HPTLC silica gel plates 60 F254 (20 × 10 cm) using acetonitrile-methanol-acetic acid-water (3.4:3.6:0.18:1 v/v) as a mobile phase containing 0.57 mM β-cyclodextrin	596:764	The optimized method involves separation on Fluka HPTLC silica gel plates 60 F254 (20 × 10 cm) using acetonitrile-methanol-acetic acid-water (3.4:3.6:0.18:1 v/v) as a mobile phase containing 0.57 mM β-cyclodextrin.
30193408	7	83	dep	band-1	1306:1311	arg1	-					1318:1318	-	1318:1318	-	1318:1318	The detection limit of betaxolol was 0.15 and 0.13 μg band-1 for (-) and (+) enantiomers, respectively.
30193408	7	84	theme	detection	1256:1264	arg1	band-1					1306:1311	0.15 and 0.13 μg band-1	1289:1311	0.15 and 0.13 μg band-1 for (-)	1289:1319	The detection limit of betaxolol was 0.15 and 0.13 μg band-1 for (-) and (+) enantiomers, respectively.
30193408	7	84	theme	detection	1256:1264	arg1	limit					1266:1270	The detection limit	1252:1270	The detection limit of betaxolol	1252:1283	The detection limit of betaxolol was 0.15 and 0.13 μg band-1 for (-) and (+) enantiomers, respectively.
30193408	5	85	theme	organic	1053:1059	arg1	part					1061:1064	the organic part	1049:1064	the organic part of the mobile phase	1049:1084	Maximum separation of the enantiomers of the three drugs was obtained by optimizing concentration of chiral selector, the mobile phase composition including acetonitrile amount in the organic part of the mobile phase and the volume of acetic acid added.
30193408	5	86	theme	phase	998:1002	arg1	composition					1004:1014	the mobile phase composition	987:1014	the mobile phase composition including acetonitrile amount in the organic part of the mobile phase	987:1084	Maximum separation of the enantiomers of the three drugs was obtained by optimizing concentration of chiral selector, the mobile phase composition including acetonitrile amount in the organic part of the mobile phase and the volume of acetic acid added.
30193408	8	87	theme	detection	1360:1368	arg1	limits					1370:1375	The detection limits	1356:1375	The detection limits	1356:1375	The detection limits were found to be 0.2 and 0.3 μg band-1 for carvedilol and sotalol, respectively, as racemate.
30193408	8	87	theme	detection	1360:1368	arg1	band-1					1409:1414	0.2 and 0.3 μg band-1	1394:1414	0.2 and 0.3 μg band-1	1394:1414	The detection limits were found to be 0.2 and 0.3 μg band-1 for carvedilol and sotalol, respectively, as racemate.
30193408	3	88	dep	plates	619:624	arg1	60 F254					626:632	60 F254	626:632	60 F254	626:632	The optimized method involves separation on Fluka HPTLC silica gel plates 60 F254 (20 × 10 cm) using acetonitrile-methanol-acetic acid-water (3.4:3.6:0.18:1 v/v) as a mobile phase containing 0.57 mM β-cyclodextrin.
30193408	3	88	dep	plates	619:624	arg1	20 × 10 cm					635:644	20 × 10 cm	635:644	20 × 10 cm	635:644	The optimized method involves separation on Fluka HPTLC silica gel plates 60 F254 (20 × 10 cm) using acetonitrile-methanol-acetic acid-water (3.4:3.6:0.18:1 v/v) as a mobile phase containing 0.57 mM β-cyclodextrin.
30193408	1	89	theme	±	258:258	arg1	betaxolol					261:269	(±) betaxolol	257:269	(±) betaxolol	257:269	Stereospecific separation method of (±) betaxolol, (±) carvedilol, and (±) sotalol using High Performance Thin Layer Chromatography (HPTLC) and β-cyclodextrin as chiral selector has been developed and validated.
31171578	0	0	theme	Metabolic	67:75	arg1	Fluxes					77:82	Metabolic Fluxes	67:82	Metabolic Fluxes in Seedlings	67:95	A Genome-Scale Metabolic Model of Soybean (Glycine max) Highlights Metabolic Fluxes in Seedlings.
31171578	6	1	from	synthesis	1311:1319	arg1	range					1270:1274	a range	1268:1274	a range of imported amino acids in protein synthesis and catabolic processes	1268:1343	In particular, from 48 h onward, cotyledons were characterized by the oxidation of fatty acids to supply carbon for the tricarboxylic acid cycle as well as production of sucrose and glutamate for export to the HRA, while the HRA was characterized by the use of a range of imported amino acids in protein synthesis and catabolic processes.
31171578	7	2	theme	new	1397:1399	arg1	insight					1401:1407	new insight	1397:1407	new insight into well-characterized metabolic processes	1397:1451	Overall, the use of flux balance modeling provided new insight into well-characterized metabolic processes in an important crop plant due to their analysis within the context of a metabolic network and reinforces the relevance of the application of this technique to the analysis of complex plant metabolic systems.
31171578	4	3	from	analysis	660:667	arg1	composition					715:725	biomass composition	707:725	biomass composition	707:725	A detailed analysis of seedling growth and alterations in biomass composition was performed over 4 d of postgerminative growth and used to constrain the stoichiometric model.
31171578	6	4	from	processes	1335:1343	arg1	range					1270:1274	a range	1268:1274	a range of imported amino acids in protein synthesis and catabolic processes	1268:1343	In particular, from 48 h onward, cotyledons were characterized by the oxidation of fatty acids to supply carbon for the tricarboxylic acid cycle as well as production of sucrose and glutamate for export to the HRA, while the HRA was characterized by the use of a range of imported amino acids in protein synthesis and catabolic processes.
31171578	6	5	theme	supply	1105:1110	arg1	carbon					1112:1117	supply carbon	1105:1117	supply carbon for the tricarboxylic acid cycle as well as production of sucrose and glutamate	1105:1197	In particular, from 48 h onward, cotyledons were characterized by the oxidation of fatty acids to supply carbon for the tricarboxylic acid cycle as well as production of sucrose and glutamate for export to the HRA, while the HRA was characterized by the use of a range of imported amino acids in protein synthesis and catabolic processes.
31171578	7	6	theme	metabolic	1643:1651	arg1	systems					1653:1659	complex plant metabolic systems	1629:1659	complex plant metabolic systems	1629:1659	Overall, the use of flux balance modeling provided new insight into well-characterized metabolic processes in an important crop plant due to their analysis within the context of a metabolic network and reinforces the relevance of the application of this technique to the analysis of complex plant metabolic systems.
31171578	6	7	theme	protein	1303:1309	arg1	synthesis					1311:1319	protein synthesis	1303:1319	protein synthesis	1303:1319	In particular, from 48 h onward, cotyledons were characterized by the oxidation of fatty acids to supply carbon for the tricarboxylic acid cycle as well as production of sucrose and glutamate for export to the HRA, while the HRA was characterized by the use of a range of imported amino acids in protein synthesis and catabolic processes.
31171578	6	8	theme	sucrose	1177:1183	arg1	production					1163:1172	production	1163:1172	supply carbon for the tricarboxylic acid cycle as well as production of sucrose and glutamate	1105:1197	In particular, from 48 h onward, cotyledons were characterized by the oxidation of fatty acids to supply carbon for the tricarboxylic acid cycle as well as production of sucrose and glutamate for export to the HRA, while the HRA was characterized by the use of a range of imported amino acids in protein synthesis and catabolic processes.
31171578	6	8	theme	sucrose	1177:1183	arg1	carbon					1112:1117	supply carbon	1105:1117	supply carbon for the tricarboxylic acid cycle as well as production of sucrose and glutamate	1105:1197	In particular, from 48 h onward, cotyledons were characterized by the oxidation of fatty acids to supply carbon for the tricarboxylic acid cycle as well as production of sucrose and glutamate for export to the HRA, while the HRA was characterized by the use of a range of imported amino acids in protein synthesis and catabolic processes.
31171578	5	9	from	shifts	926:931	arg1	metabolism					944:953	primary metabolism	936:953	primary metabolism occurring during different periods postgermination	936:1004	Flux balance analysis revealed marked differences in metabolism between the two organs, together with shifts in primary metabolism occurring during different periods postgermination.
31171578	2	10	theme	breakdown	269:277	arg1	products					279:286	their breakdown products	263:286	their breakdown products	263:286	These reserves must be mobilized and metabolized, and their breakdown products must be distributed to the different organs of the growing seedling.
31171578	7	11	theme	systems	1653:1659	arg1	analysis					1617:1624	the analysis	1613:1624	the analysis of complex plant metabolic systems	1613:1659	Overall, the use of flux balance modeling provided new insight into well-characterized metabolic processes in an important crop plant due to their analysis within the context of a metabolic network and reinforces the relevance of the application of this technique to the analysis of complex plant metabolic systems.
31171578	6	12	theme	acid	1141:1144	arg1	cycle					1146:1150	the tricarboxylic acid cycle	1123:1150	the tricarboxylic acid cycle	1123:1150	In particular, from 48 h onward, cotyledons were characterized by the oxidation of fatty acids to supply carbon for the tricarboxylic acid cycle as well as production of sucrose and glutamate for export to the HRA, while the HRA was characterized by the use of a range of imported amino acids in protein synthesis and catabolic processes.
31171578	6	13	from	acids	1294:1298	arg1	synthesis					1311:1319	protein synthesis	1303:1319	protein synthesis	1303:1319	In particular, from 48 h onward, cotyledons were characterized by the oxidation of fatty acids to supply carbon for the tricarboxylic acid cycle as well as production of sucrose and glutamate for export to the HRA, while the HRA was characterized by the use of a range of imported amino acids in protein synthesis and catabolic processes.
31171578	6	13	from	acids	1294:1298	arg1	processes					1335:1343	catabolic processes	1325:1343	catabolic processes	1325:1343	In particular, from 48 h onward, cotyledons were characterized by the oxidation of fatty acids to supply carbon for the tricarboxylic acid cycle as well as production of sucrose and glutamate for export to the HRA, while the HRA was characterized by the use of a range of imported amino acids in protein synthesis and catabolic processes.
31171578	7	14	theme	balance	1371:1377	arg1	modeling					1379:1386	flux balance modeling	1366:1386	flux balance modeling	1366:1386	Overall, the use of flux balance modeling provided new insight into well-characterized metabolic processes in an important crop plant due to their analysis within the context of a metabolic network and reinforces the relevance of the application of this technique to the analysis of complex plant metabolic systems.
31171578	5	15	from	differences	862:872	arg1	metabolism					877:886	metabolism	877:886	metabolism	877:886	Flux balance analysis revealed marked differences in metabolism between the two organs, together with shifts in primary metabolism occurring during different periods postgermination.
31171578	3	16	theme	important	529:537	arg1	plant					544:548	this important crop plant	524:548	this important crop plant	524:548	Here, we investigated the mobilization of soybean (Glycine max) seed reserves during seedling growth by initially constructing a genome-scale stoichiometric model for this important crop plant and then adapting the model to reflect metabolism in the cotyledons and hypocotyl/root axis (HRA).
31171578	7	17	theme	complex	1629:1635	arg1	systems					1653:1659	complex plant metabolic systems	1629:1659	complex plant metabolic systems	1629:1659	Overall, the use of flux balance modeling provided new insight into well-characterized metabolic processes in an important crop plant due to their analysis within the context of a metabolic network and reinforces the relevance of the application of this technique to the analysis of complex plant metabolic systems.
31171578	5	18	theme	balance	829:835	arg1	analysis					837:844	Flux balance analysis	824:844	Flux balance analysis	824:844	Flux balance analysis revealed marked differences in metabolism between the two organs, together with shifts in primary metabolism occurring during different periods postgermination.
31171578	6	19	theme	acids	1294:1298	arg1	range					1270:1274	a range	1268:1274	a range of imported amino acids in protein synthesis and catabolic processes	1268:1343	In particular, from 48 h onward, cotyledons were characterized by the oxidation of fatty acids to supply carbon for the tricarboxylic acid cycle as well as production of sucrose and glutamate for export to the HRA, while the HRA was characterized by the use of a range of imported amino acids in protein synthesis and catabolic processes.
31171578	3	20	from	metabolism	589:598	arg1	cotyledons					607:616	the cotyledons	603:616	the cotyledons	603:616	Here, we investigated the mobilization of soybean (Glycine max) seed reserves during seedling growth by initially constructing a genome-scale stoichiometric model for this important crop plant and then adapting the model to reflect metabolism in the cotyledons and hypocotyl/root axis (HRA).
31171578	3	21	theme	Glycine	408:414	arg1	soybean					399:405	soybean	399:405	soybean (Glycine max) seed reserves	399:433	Here, we investigated the mobilization of soybean (Glycine max) seed reserves during seedling growth by initially constructing a genome-scale stoichiometric model for this important crop plant and then adapting the model to reflect metabolism in the cotyledons and hypocotyl/root axis (HRA).
31171578	3	21	theme	Glycine	408:414	arg1	max					416:418	Glycine max	408:418	Glycine max	408:418	Here, we investigated the mobilization of soybean (Glycine max) seed reserves during seedling growth by initially constructing a genome-scale stoichiometric model for this important crop plant and then adapting the model to reflect metabolism in the cotyledons and hypocotyl/root axis (HRA).
31171578	3	22	from	axis	637:640	arg1	cotyledons					607:616	the cotyledons	603:616	the cotyledons	603:616	Here, we investigated the mobilization of soybean (Glycine max) seed reserves during seedling growth by initially constructing a genome-scale stoichiometric model for this important crop plant and then adapting the model to reflect metabolism in the cotyledons and hypocotyl/root axis (HRA).
31171578	7	23	theme	modeling	1379:1386	arg1	use					1359:1361	the use	1355:1361	the use of flux balance modeling	1355:1386	Overall, the use of flux balance modeling provided new insight into well-characterized metabolic processes in an important crop plant due to their analysis within the context of a metabolic network and reinforces the relevance of the application of this technique to the analysis of complex plant metabolic systems.
31171578	4	24	theme	stoichiometric	802:815	arg1	model					817:821	the stoichiometric model	798:821	the stoichiometric model	798:821	A detailed analysis of seedling growth and alterations in biomass composition was performed over 4 d of postgerminative growth and used to constrain the stoichiometric model.
31171578	7	25	theme	network	1536:1542	arg1	context					1513:1519	the context	1509:1519	the context of a metabolic network	1509:1542	Overall, the use of flux balance modeling provided new insight into well-characterized metabolic processes in an important crop plant due to their analysis within the context of a metabolic network and reinforces the relevance of the application of this technique to the analysis of complex plant metabolic systems.
31171578	4	26	used	used	780:783	arg2	analysis					660:667	A detailed analysis	649:667	A detailed analysis of seedling growth and alterations in biomass composition	649:725	A detailed analysis of seedling growth and alterations in biomass composition was performed over 4 d of postgerminative growth and used to constrain the stoichiometric model.
31171578	7	27	theme	well-characterized	1414:1431	arg1	processes					1443:1451	well-characterized metabolic processes	1414:1451	well-characterized metabolic processes	1414:1451	Overall, the use of flux balance modeling provided new insight into well-characterized metabolic processes in an important crop plant due to their analysis within the context of a metabolic network and reinforces the relevance of the application of this technique to the analysis of complex plant metabolic systems.
31171578	0	28	theme	Genome-Scale	2:13	arg1	max					51:53	Glycine max	43:53	Glycine max	43:53	A Genome-Scale Metabolic Model of Soybean (Glycine max) Highlights Metabolic Fluxes in Seedlings.
31171578	0	28	theme	Genome-Scale	2:13	arg1	Model					25:29	A Genome-Scale Metabolic Model	0:29	A Genome-Scale Metabolic Model of Soybean (Glycine max)	0:54	A Genome-Scale Metabolic Model of Soybean (Glycine max) Highlights Metabolic Fluxes in Seedlings.
31171578	4	29	from	composition	715:725	arg1	analysis					660:667	A detailed analysis	649:667	A detailed analysis of seedling growth and alterations in biomass composition	649:725	A detailed analysis of seedling growth and alterations in biomass composition was performed over 4 d of postgerminative growth and used to constrain the stoichiometric model.
31171578	4	30	theme	biomass	707:713	arg1	composition					715:725	biomass composition	707:725	biomass composition	707:725	A detailed analysis of seedling growth and alterations in biomass composition was performed over 4 d of postgerminative growth and used to constrain the stoichiometric model.
31171578	3	31	theme	soybean	399:405	arg1	reserves					426:433	soybean (Glycine max) seed reserves	399:433	soybean (Glycine max) seed reserves	399:433	Here, we investigated the mobilization of soybean (Glycine max) seed reserves during seedling growth by initially constructing a genome-scale stoichiometric model for this important crop plant and then adapting the model to reflect metabolism in the cotyledons and hypocotyl/root axis (HRA).
31171578	6	32	from	range	1270:1274	arg1	synthesis					1311:1319	protein synthesis	1303:1319	protein synthesis	1303:1319	In particular, from 48 h onward, cotyledons were characterized by the oxidation of fatty acids to supply carbon for the tricarboxylic acid cycle as well as production of sucrose and glutamate for export to the HRA, while the HRA was characterized by the use of a range of imported amino acids in protein synthesis and catabolic processes.
31171578	6	32	from	range	1270:1274	arg1	processes					1335:1343	catabolic processes	1325:1343	catabolic processes	1325:1343	In particular, from 48 h onward, cotyledons were characterized by the oxidation of fatty acids to supply carbon for the tricarboxylic acid cycle as well as production of sucrose and glutamate for export to the HRA, while the HRA was characterized by the use of a range of imported amino acids in protein synthesis and catabolic processes.
31171578	6	33	theme	tricarboxylic	1127:1139	arg1	cycle					1146:1150	the tricarboxylic acid cycle	1123:1150	the tricarboxylic acid cycle	1123:1150	In particular, from 48 h onward, cotyledons were characterized by the oxidation of fatty acids to supply carbon for the tricarboxylic acid cycle as well as production of sucrose and glutamate for export to the HRA, while the HRA was characterized by the use of a range of imported amino acids in protein synthesis and catabolic processes.
31171578	5	34	theme	marked	855:860	arg1	differences					862:872	marked differences	855:872	marked differences in metabolism between the two organs	855:909	Flux balance analysis revealed marked differences in metabolism between the two organs, together with shifts in primary metabolism occurring during different periods postgermination.
31171578	6	35	theme	amino	1288:1292	arg1	acids					1294:1298	imported amino acids	1279:1298	imported amino acids in protein synthesis and catabolic processes	1279:1343	In particular, from 48 h onward, cotyledons were characterized by the oxidation of fatty acids to supply carbon for the tricarboxylic acid cycle as well as production of sucrose and glutamate for export to the HRA, while the HRA was characterized by the use of a range of imported amino acids in protein synthesis and catabolic processes.
31171578	3	36	theme	crop	539:542	arg1	plant					544:548	this important crop plant	524:548	this important crop plant	524:548	Here, we investigated the mobilization of soybean (Glycine max) seed reserves during seedling growth by initially constructing a genome-scale stoichiometric model for this important crop plant and then adapting the model to reflect metabolism in the cotyledons and hypocotyl/root axis (HRA).
31171578	3	37	theme	reserves	426:433	arg1	mobilization					383:394	the mobilization	379:394	the mobilization of soybean (Glycine max) seed reserves	379:433	Here, we investigated the mobilization of soybean (Glycine max) seed reserves during seedling growth by initially constructing a genome-scale stoichiometric model for this important crop plant and then adapting the model to reflect metabolism in the cotyledons and hypocotyl/root axis (HRA).
31171578	0	38	theme	Metabolic	15:23	arg1	max					51:53	Glycine max	43:53	Glycine max	43:53	A Genome-Scale Metabolic Model of Soybean (Glycine max) Highlights Metabolic Fluxes in Seedlings.
31171578	0	38	theme	Metabolic	15:23	arg1	Model					25:29	A Genome-Scale Metabolic Model	0:29	A Genome-Scale Metabolic Model of Soybean (Glycine max)	0:54	A Genome-Scale Metabolic Model of Soybean (Glycine max) Highlights Metabolic Fluxes in Seedlings.
31171578	3	39	theme	hypocotyl/root	622:635	arg1	HRA					643:645	HRA	643:645	HRA	643:645	Here, we investigated the mobilization of soybean (Glycine max) seed reserves during seedling growth by initially constructing a genome-scale stoichiometric model for this important crop plant and then adapting the model to reflect metabolism in the cotyledons and hypocotyl/root axis (HRA).
31171578	3	39	theme	hypocotyl/root	622:635	arg1	axis					637:640	hypocotyl/root axis	622:640	hypocotyl/root axis (HRA)	622:646	Here, we investigated the mobilization of soybean (Glycine max) seed reserves during seedling growth by initially constructing a genome-scale stoichiometric model for this important crop plant and then adapting the model to reflect metabolism in the cotyledons and hypocotyl/root axis (HRA).
31171578	7	40	theme	technique	1600:1608	arg1	application					1580:1590	the application	1576:1590	the application of this technique to the analysis of complex plant metabolic systems	1576:1659	Overall, the use of flux balance modeling provided new insight into well-characterized metabolic processes in an important crop plant due to their analysis within the context of a metabolic network and reinforces the relevance of the application of this technique to the analysis of complex plant metabolic systems.
31171578	3	41	theme	genome-scale	486:497	arg1	model					514:518	a genome-scale stoichiometric model	484:518	a genome-scale stoichiometric model for this important crop plant	484:548	Here, we investigated the mobilization of soybean (Glycine max) seed reserves during seedling growth by initially constructing a genome-scale stoichiometric model for this important crop plant and then adapting the model to reflect metabolism in the cotyledons and hypocotyl/root axis (HRA).
31171578	0	42	theme	Soybean	34:40	arg1	max					51:53	Glycine max	43:53	Glycine max	43:53	A Genome-Scale Metabolic Model of Soybean (Glycine max) Highlights Metabolic Fluxes in Seedlings.
31171578	0	42	theme	Soybean	34:40	arg1	Model					25:29	A Genome-Scale Metabolic Model	0:29	A Genome-Scale Metabolic Model of Soybean (Glycine max)	0:54	A Genome-Scale Metabolic Model of Soybean (Glycine max) Highlights Metabolic Fluxes in Seedlings.
31171578	4	43	theme	seedling	672:679	arg1	growth					681:686	seedling growth	672:686	seedling growth	672:686	A detailed analysis of seedling growth and alterations in biomass composition was performed over 4 d of postgerminative growth and used to constrain the stoichiometric model.
31171578	6	44	theme	range	1270:1274	arg1	use					1261:1263	the use	1257:1263	the use of a range of imported amino acids in protein synthesis and catabolic processes	1257:1343	In particular, from 48 h onward, cotyledons were characterized by the oxidation of fatty acids to supply carbon for the tricarboxylic acid cycle as well as production of sucrose and glutamate for export to the HRA, while the HRA was characterized by the use of a range of imported amino acids in protein synthesis and catabolic processes.
31171578	6	45	theme	h	1030:1030	arg1	onward					1032:1037	48 h onward	1027:1037	48 h onward	1027:1037	In particular, from 48 h onward, cotyledons were characterized by the oxidation of fatty acids to supply carbon for the tricarboxylic acid cycle as well as production of sucrose and glutamate for export to the HRA, while the HRA was characterized by the use of a range of imported amino acids in protein synthesis and catabolic processes.
31171578	7	46	theme	crop	1469:1472	arg1	plant					1474:1478	an important crop plant	1456:1478	an important crop plant due to their analysis within the context of a metabolic network	1456:1542	Overall, the use of flux balance modeling provided new insight into well-characterized metabolic processes in an important crop plant due to their analysis within the context of a metabolic network and reinforces the relevance of the application of this technique to the analysis of complex plant metabolic systems.
31171578	1	47	theme	seed	195:198	arg1	tissues					200:206	seed tissues	195:206	seed tissues	195:206	Until they become photoautotrophic juvenile plants, seedlings depend upon the reserves stored in seed tissues.
31171578	4	48	theme	detailed	651:658	arg1	analysis					660:667	A detailed analysis	649:667	A detailed analysis of seedling growth and alterations in biomass composition	649:725	A detailed analysis of seedling growth and alterations in biomass composition was performed over 4 d of postgerminative growth and used to constrain the stoichiometric model.
31171578	7	49	theme	plant	1637:1641	arg1	systems					1653:1659	complex plant metabolic systems	1629:1659	complex plant metabolic systems	1629:1659	Overall, the use of flux balance modeling provided new insight into well-characterized metabolic processes in an important crop plant due to their analysis within the context of a metabolic network and reinforces the relevance of the application of this technique to the analysis of complex plant metabolic systems.
31171578	5	50	theme	Flux	824:827	arg1	analysis					837:844	Flux balance analysis	824:844	Flux balance analysis	824:844	Flux balance analysis revealed marked differences in metabolism between the two organs, together with shifts in primary metabolism occurring during different periods postgermination.
31171578	3	51	theme	seed	421:424	arg1	reserves					426:433	soybean (Glycine max) seed reserves	399:433	soybean (Glycine max) seed reserves	399:433	Here, we investigated the mobilization of soybean (Glycine max) seed reserves during seedling growth by initially constructing a genome-scale stoichiometric model for this important crop plant and then adapting the model to reflect metabolism in the cotyledons and hypocotyl/root axis (HRA).
31171578	0	52	theme	Glycine	43:49	arg1	max					51:53	Glycine max	43:53	Glycine max	43:53	A Genome-Scale Metabolic Model of Soybean (Glycine max) Highlights Metabolic Fluxes in Seedlings.
31171578	0	52	theme	Glycine	43:49	arg1	Model					25:29	A Genome-Scale Metabolic Model	0:29	A Genome-Scale Metabolic Model of Soybean (Glycine max)	0:54	A Genome-Scale Metabolic Model of Soybean (Glycine max) Highlights Metabolic Fluxes in Seedlings.
31171578	7	53	theme	due	1480:1482	arg1	plant					1474:1478	an important crop plant	1456:1478	an important crop plant due to their analysis within the context of a metabolic network	1456:1542	Overall, the use of flux balance modeling provided new insight into well-characterized metabolic processes in an important crop plant due to their analysis within the context of a metabolic network and reinforces the relevance of the application of this technique to the analysis of complex plant metabolic systems.
31171578	6	54	theme	48	1027:1028	arg1	h					1030:1030	h	1030:1030	h	1030:1030	In particular, from 48 h onward, cotyledons were characterized by the oxidation of fatty acids to supply carbon for the tricarboxylic acid cycle as well as production of sucrose and glutamate for export to the HRA, while the HRA was characterized by the use of a range of imported amino acids in protein synthesis and catabolic processes.
31171578	6	55	theme	catabolic	1325:1333	arg1	processes					1335:1343	catabolic processes	1325:1343	catabolic processes	1325:1343	In particular, from 48 h onward, cotyledons were characterized by the oxidation of fatty acids to supply carbon for the tricarboxylic acid cycle as well as production of sucrose and glutamate for export to the HRA, while the HRA was characterized by the use of a range of imported amino acids in protein synthesis and catabolic processes.
31171578	6	56	theme	fatty	1090:1094	arg1	acids					1096:1100	fatty acids	1090:1100	fatty acids	1090:1100	In particular, from 48 h onward, cotyledons were characterized by the oxidation of fatty acids to supply carbon for the tricarboxylic acid cycle as well as production of sucrose and glutamate for export to the HRA, while the HRA was characterized by the use of a range of imported amino acids in protein synthesis and catabolic processes.
31171578	4	57	theme	growth	681:686	arg1	analysis					660:667	A detailed analysis	649:667	A detailed analysis of seedling growth and alterations in biomass composition	649:725	A detailed analysis of seedling growth and alterations in biomass composition was performed over 4 d of postgerminative growth and used to constrain the stoichiometric model.
31171578	2	58	theme	growing	339:345	arg1	seedling					347:354	the growing seedling	335:354	the growing seedling	335:354	These reserves must be mobilized and metabolized, and their breakdown products must be distributed to the different organs of the growing seedling.
31171578	6	59	theme	imported	1279:1286	arg1	acids					1294:1298	imported amino acids	1279:1298	imported amino acids in protein synthesis and catabolic processes	1279:1343	In particular, from 48 h onward, cotyledons were characterized by the oxidation of fatty acids to supply carbon for the tricarboxylic acid cycle as well as production of sucrose and glutamate for export to the HRA, while the HRA was characterized by the use of a range of imported amino acids in protein synthesis and catabolic processes.
31171578	7	60	theme	important	1459:1467	arg1	plant					1474:1478	an important crop plant	1456:1478	an important crop plant due to their analysis within the context of a metabolic network	1456:1542	Overall, the use of flux balance modeling provided new insight into well-characterized metabolic processes in an important crop plant due to their analysis within the context of a metabolic network and reinforces the relevance of the application of this technique to the analysis of complex plant metabolic systems.
31171578	6	61	theme	glutamate	1189:1197	arg1	production					1163:1172	production	1163:1172	supply carbon for the tricarboxylic acid cycle as well as production of sucrose and glutamate	1105:1197	In particular, from 48 h onward, cotyledons were characterized by the oxidation of fatty acids to supply carbon for the tricarboxylic acid cycle as well as production of sucrose and glutamate for export to the HRA, while the HRA was characterized by the use of a range of imported amino acids in protein synthesis and catabolic processes.
31171578	6	61	theme	glutamate	1189:1197	arg1	carbon					1112:1117	supply carbon	1105:1117	supply carbon for the tricarboxylic acid cycle as well as production of sucrose and glutamate	1105:1197	In particular, from 48 h onward, cotyledons were characterized by the oxidation of fatty acids to supply carbon for the tricarboxylic acid cycle as well as production of sucrose and glutamate for export to the HRA, while the HRA was characterized by the use of a range of imported amino acids in protein synthesis and catabolic processes.
31171578	2	62	theme	different	315:323	arg1	organs					325:330	the different organs	311:330	the different organs of the growing seedling	311:354	These reserves must be mobilized and metabolized, and their breakdown products must be distributed to the different organs of the growing seedling.
31171578	4	63	from	alterations	692:702	arg1	composition					715:725	biomass composition	707:725	biomass composition	707:725	A detailed analysis of seedling growth and alterations in biomass composition was performed over 4 d of postgerminative growth and used to constrain the stoichiometric model.
31171578	7	64	theme	flux	1366:1369	arg1	modeling					1379:1386	flux balance modeling	1366:1386	flux balance modeling	1366:1386	Overall, the use of flux balance modeling provided new insight into well-characterized metabolic processes in an important crop plant due to their analysis within the context of a metabolic network and reinforces the relevance of the application of this technique to the analysis of complex plant metabolic systems.
31171578	1	65	theme	juvenile	133:140	arg1	plants					142:147	photoautotrophic juvenile plants	116:147	photoautotrophic juvenile plants	116:147	Until they become photoautotrophic juvenile plants, seedlings depend upon the reserves stored in seed tissues.
31171578	4	66	theme	postgerminative	753:767	arg1	growth					769:774	postgerminative growth	753:774	postgerminative growth	753:774	A detailed analysis of seedling growth and alterations in biomass composition was performed over 4 d of postgerminative growth and used to constrain the stoichiometric model.
31171578	3	67	theme	seedling	442:449	arg1	growth					451:456	seedling growth	442:456	seedling growth	442:456	Here, we investigated the mobilization of soybean (Glycine max) seed reserves during seedling growth by initially constructing a genome-scale stoichiometric model for this important crop plant and then adapting the model to reflect metabolism in the cotyledons and hypocotyl/root axis (HRA).
31171578	6	68	theme	acids	1096:1100	arg1	oxidation					1077:1085	the oxidation	1073:1085	the oxidation of fatty acids to supply carbon for the tricarboxylic acid cycle as well as production of sucrose and glutamate	1073:1197	In particular, from 48 h onward, cotyledons were characterized by the oxidation of fatty acids to supply carbon for the tricarboxylic acid cycle as well as production of sucrose and glutamate for export to the HRA, while the HRA was characterized by the use of a range of imported amino acids in protein synthesis and catabolic processes.
31171578	7	69	theme	metabolic	1526:1534	arg1	network					1536:1542	a metabolic network	1524:1542	a metabolic network	1524:1542	Overall, the use of flux balance modeling provided new insight into well-characterized metabolic processes in an important crop plant due to their analysis within the context of a metabolic network and reinforces the relevance of the application of this technique to the analysis of complex plant metabolic systems.
31171578	7	70	theme	application	1580:1590	arg1	relevance					1563:1571	the relevance	1559:1571	the relevance of the application of this technique to the analysis of complex plant metabolic systems	1559:1659	Overall, the use of flux balance modeling provided new insight into well-characterized metabolic processes in an important crop plant due to their analysis within the context of a metabolic network and reinforces the relevance of the application of this technique to the analysis of complex plant metabolic systems.
31171578	5	71	theme	primary	936:942	arg1	metabolism					944:953	primary metabolism	936:953	primary metabolism occurring during different periods postgermination	936:1004	Flux balance analysis revealed marked differences in metabolism between the two organs, together with shifts in primary metabolism occurring during different periods postgermination.
31171578	1	72	theme	photoautotrophic	116:131	arg1	plants					142:147	photoautotrophic juvenile plants	116:147	photoautotrophic juvenile plants	116:147	Until they become photoautotrophic juvenile plants, seedlings depend upon the reserves stored in seed tissues.
31171578	4	73	from	growth	681:686	arg1	composition					715:725	biomass composition	707:725	biomass composition	707:725	A detailed analysis of seedling growth and alterations in biomass composition was performed over 4 d of postgerminative growth and used to constrain the stoichiometric model.
31171578	4	74	theme	alterations	692:702	arg1	analysis					660:667	A detailed analysis	649:667	A detailed analysis of seedling growth and alterations in biomass composition	649:725	A detailed analysis of seedling growth and alterations in biomass composition was performed over 4 d of postgerminative growth and used to constrain the stoichiometric model.
31171578	0	75	from	Fluxes	77:82	arg1	Seedlings					87:95	Seedlings	87:95	Seedlings	87:95	A Genome-Scale Metabolic Model of Soybean (Glycine max) Highlights Metabolic Fluxes in Seedlings.
31171578	2	76	theme	seedling	347:354	arg1	organs					325:330	the different organs	311:330	the different organs of the growing seedling	311:354	These reserves must be mobilized and metabolized, and their breakdown products must be distributed to the different organs of the growing seedling.
31171578	5	77	theme	different	972:980	arg1	periods					982:988	different periods	972:988	different periods postgermination	972:1004	Flux balance analysis revealed marked differences in metabolism between the two organs, together with shifts in primary metabolism occurring during different periods postgermination.
31171578	7	78	theme	metabolic	1433:1441	arg1	processes					1443:1451	well-characterized metabolic processes	1414:1451	well-characterized metabolic processes	1414:1451	Overall, the use of flux balance modeling provided new insight into well-characterized metabolic processes in an important crop plant due to their analysis within the context of a metabolic network and reinforces the relevance of the application of this technique to the analysis of complex plant metabolic systems.
31171578	4	79	theme	growth	769:774	arg1	d					748:748	4 d	746:748	4 d of postgerminative growth	746:774	A detailed analysis of seedling growth and alterations in biomass composition was performed over 4 d of postgerminative growth and used to constrain the stoichiometric model.
31171578	3	80	theme	stoichiometric	499:512	arg1	model					514:518	a genome-scale stoichiometric model	484:518	a genome-scale stoichiometric model for this important crop plant	484:548	Here, we investigated the mobilization of soybean (Glycine max) seed reserves during seedling growth by initially constructing a genome-scale stoichiometric model for this important crop plant and then adapting the model to reflect metabolism in the cotyledons and hypocotyl/root axis (HRA).
30953001	0	0	theme	colonic	92:98	arg1	composition					110:120	the colonic bacterial composition	88:120	the colonic bacterial composition	88:120	Deficiency in intestinal epithelial Reg4 ameliorates intestinal inflammation and alters the colonic bacterial composition.
30953001	1	1	link	islet-derived	140:152	arg1	member					161:166	The regenerating islet-derived family member 4	123:168	The regenerating islet-derived family member 4 (Reg4) in the gastrointestinal tract	123:205	The regenerating islet-derived family member 4 (Reg4) in the gastrointestinal tract is up-regulated during intestinal inflammation.
30953001	1	1	link	islet-derived	140:152	arg1	Reg4					171:174	Reg4	171:174	Reg4	171:174	The regenerating islet-derived family member 4 (Reg4) in the gastrointestinal tract is up-regulated during intestinal inflammation.
30953001	4	2	theme	pediatric	702:710	arg1	patients					736:743	pediatric intestinal failure (IF) patients	702:743	pediatric intestinal failure (IF) patients	702:743	We showed that the elevated serum Reg4 in pediatric intestinal failure (IF) patients were positively correlated with the serum concentrations of proinflammatory cytokines interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α).
30953001	10	3	theme	experimental	1654:1665	arg1	colitis					1667:1673	experimental colitis	1654:1673	experimental colitis	1654:1673	In conclusion, our findings demonstrated intestinal-epithelial Reg4 deficiency protects against experimental colitis and mucosal injury via a mechanism involving alteration of bacterial homeostasis and STAT3 activation.
30953001	10	4	theme	mucosal	1679:1685	arg1	injury					1687:1692	mucosal injury	1679:1692	mucosal injury	1679:1692	In conclusion, our findings demonstrated intestinal-epithelial Reg4 deficiency protects against experimental colitis and mucosal injury via a mechanism involving alteration of bacterial homeostasis and STAT3 activation.
30953001	6	5	theme	transcription	1071:1083	arg1	factor					1085:1090	activating transcription factor 2	1060:1092	activating transcription factor 2 (ATF2)	1060:1099	The Reg4 was indicated as a novel target of activating transcription factor 2 (ATF2) that enhanced Reg4 expression during the intestinal inflammation.
30953001	6	5	theme	transcription	1071:1083	arg1	ATF2					1095:1098	ATF2	1095:1098	ATF2	1095:1098	The Reg4 was indicated as a novel target of activating transcription factor 2 (ATF2) that enhanced Reg4 expression during the intestinal inflammation.
30953001	4	6	from	Reg4	694:697	arg1	patients					736:743	pediatric intestinal failure (IF) patients	702:743	pediatric intestinal failure (IF) patients	702:743	We showed that the elevated serum Reg4 in pediatric intestinal failure (IF) patients were positively correlated with the serum concentrations of proinflammatory cytokines interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α).
30953001	3	7	theme	functional	365:374	arg1	roles					376:380	the functional roles	361:380	the functional roles	361:380	In the current study, the functional roles and involved mechanisms of intestinal epithelial Reg4 in intestinal inflammation were studied in healthy and inflamed states using human intestinal specimens, an intestinal conditional Reg4 knockout mouse (Reg4ΔIEC) model and dextran sulfate sodium (DSS)-induced colitis model.
30953001	0	8	from	Deficiency	0:9	arg1	Reg4					36:39	intestinal epithelial Reg4	14:39	intestinal epithelial Reg4	14:39	Deficiency in intestinal epithelial Reg4 ameliorates intestinal inflammation and alters the colonic bacterial composition.
30953001	6	9	theme	intestinal	1142:1151	arg1	inflammation					1153:1164	the intestinal inflammation	1138:1164	the intestinal inflammation	1138:1164	The Reg4 was indicated as a novel target of activating transcription factor 2 (ATF2) that enhanced Reg4 expression during the intestinal inflammation.
30953001	3	10	theme	knockout	572:579	arg1	specimens					530:538	human intestinal specimens	513:538	human intestinal specimens	513:538	In the current study, the functional roles and involved mechanisms of intestinal epithelial Reg4 in intestinal inflammation were studied in healthy and inflamed states using human intestinal specimens, an intestinal conditional Reg4 knockout mouse (Reg4ΔIEC) model and dextran sulfate sodium (DSS)-induced colitis model.
30953001	3	10	theme	knockout	572:579	arg1	model					598:602	an intestinal conditional Reg4 knockout mouse (Reg4ΔIEC) model	541:602	an intestinal conditional Reg4 knockout mouse (Reg4ΔIEC) model	541:602	In the current study, the functional roles and involved mechanisms of intestinal epithelial Reg4 in intestinal inflammation were studied in healthy and inflamed states using human intestinal specimens, an intestinal conditional Reg4 knockout mouse (Reg4ΔIEC) model and dextran sulfate sodium (DSS)-induced colitis model.
30953001	6	11	theme	Reg4	1115:1118	arg1	expression					1120:1129	Reg4 expression	1115:1129	Reg4 expression	1115:1129	The Reg4 was indicated as a novel target of activating transcription factor 2 (ATF2) that enhanced Reg4 expression during the intestinal inflammation.
30953001	0	12	theme	bacterial	100:108	arg1	composition					110:120	the colonic bacterial composition	88:120	the colonic bacterial composition	88:120	Deficiency in intestinal epithelial Reg4 ameliorates intestinal inflammation and alters the colonic bacterial composition.
30953001	10	13	theme	bacterial	1734:1742	arg1	homeostasis					1744:1754	bacterial homeostasis	1734:1754	bacterial homeostasis	1734:1754	In conclusion, our findings demonstrated intestinal-epithelial Reg4 deficiency protects against experimental colitis and mucosal injury via a mechanism involving alteration of bacterial homeostasis and STAT3 activation.
30953001	7	14	theme	Reg4ΔIEC	1233:1240	arg1	mice					1242:1245	Reg4ΔIEC mice	1233:1245	Reg4ΔIEC mice	1233:1245	In vivo, the DSS-induced colitis was significantly ameliorated in Reg4ΔIEC mice.
30953001	3	15	theme	mouse	581:585	arg1	specimens					530:538	human intestinal specimens	513:538	human intestinal specimens	513:538	In the current study, the functional roles and involved mechanisms of intestinal epithelial Reg4 in intestinal inflammation were studied in healthy and inflamed states using human intestinal specimens, an intestinal conditional Reg4 knockout mouse (Reg4ΔIEC) model and dextran sulfate sodium (DSS)-induced colitis model.
30953001	3	15	theme	mouse	581:585	arg1	model					598:602	an intestinal conditional Reg4 knockout mouse (Reg4ΔIEC) model	541:602	an intestinal conditional Reg4 knockout mouse (Reg4ΔIEC) model	541:602	In the current study, the functional roles and involved mechanisms of intestinal epithelial Reg4 in intestinal inflammation were studied in healthy and inflamed states using human intestinal specimens, an intestinal conditional Reg4 knockout mouse (Reg4ΔIEC) model and dextran sulfate sodium (DSS)-induced colitis model.
30953001	3	16	theme	human	513:517	arg1	model					653:657	dextran sulfate sodium (DSS)-induced colitis model	608:657	dextran sulfate sodium (DSS)-induced colitis model	608:657	In the current study, the functional roles and involved mechanisms of intestinal epithelial Reg4 in intestinal inflammation were studied in healthy and inflamed states using human intestinal specimens, an intestinal conditional Reg4 knockout mouse (Reg4ΔIEC) model and dextran sulfate sodium (DSS)-induced colitis model.
30953001	3	16	theme	human	513:517	arg1	specimens					530:538	human intestinal specimens	513:538	human intestinal specimens	513:538	In the current study, the functional roles and involved mechanisms of intestinal epithelial Reg4 in intestinal inflammation were studied in healthy and inflamed states using human intestinal specimens, an intestinal conditional Reg4 knockout mouse (Reg4ΔIEC) model and dextran sulfate sodium (DSS)-induced colitis model.
30953001	3	16	theme	human	513:517	arg1	model					598:602	an intestinal conditional Reg4 knockout mouse (Reg4ΔIEC) model	541:602	an intestinal conditional Reg4 knockout mouse (Reg4ΔIEC) model	541:602	In the current study, the functional roles and involved mechanisms of intestinal epithelial Reg4 in intestinal inflammation were studied in healthy and inflamed states using human intestinal specimens, an intestinal conditional Reg4 knockout mouse (Reg4ΔIEC) model and dextran sulfate sodium (DSS)-induced colitis model.
30953001	4	17	theme	tumor	856:860	arg1	necrosis					862:869	tumor necrosis factor-α 	856:879	tumor necrosis factor-α (TNF-α)	856:886	We showed that the elevated serum Reg4 in pediatric intestinal failure (IF) patients were positively correlated with the serum concentrations of proinflammatory cytokines interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α).
30953001	4	17	theme	tumor	856:860	arg1	TNF-α					881:885	TNF-α	881:885	TNF-α	881:885	We showed that the elevated serum Reg4 in pediatric intestinal failure (IF) patients were positively correlated with the serum concentrations of proinflammatory cytokines interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α).
30953001	4	17	theme	tumor	856:860	arg1	cytokines					821:829	proinflammatory cytokines	805:829	proinflammatory cytokines interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α)	805:886	We showed that the elevated serum Reg4 in pediatric intestinal failure (IF) patients were positively correlated with the serum concentrations of proinflammatory cytokines interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α).
30953001	6	18	theme	factor	1085:1090	arg1	target					1050:1055	a novel target	1042:1055	a novel target of activating transcription factor 2 (ATF2) that enhanced Reg4 expression during the intestinal inflammation	1042:1164	The Reg4 was indicated as a novel target of activating transcription factor 2 (ATF2) that enhanced Reg4 expression during the intestinal inflammation.
30953001	6	18	theme	factor	1085:1090	arg1	Reg4					1020:1023	The Reg4	1016:1023	The Reg4	1016:1023	The Reg4 was indicated as a novel target of activating transcription factor 2 (ATF2) that enhanced Reg4 expression during the intestinal inflammation.
30953001	3	19	theme	conditional	555:565	arg1	specimens					530:538	human intestinal specimens	513:538	human intestinal specimens	513:538	In the current study, the functional roles and involved mechanisms of intestinal epithelial Reg4 in intestinal inflammation were studied in healthy and inflamed states using human intestinal specimens, an intestinal conditional Reg4 knockout mouse (Reg4ΔIEC) model and dextran sulfate sodium (DSS)-induced colitis model.
30953001	3	19	theme	conditional	555:565	arg1	model					598:602	an intestinal conditional Reg4 knockout mouse (Reg4ΔIEC) model	541:602	an intestinal conditional Reg4 knockout mouse (Reg4ΔIEC) model	541:602	In the current study, the functional roles and involved mechanisms of intestinal epithelial Reg4 in intestinal inflammation were studied in healthy and inflamed states using human intestinal specimens, an intestinal conditional Reg4 knockout mouse (Reg4ΔIEC) model and dextran sulfate sodium (DSS)-induced colitis model.
30953001	3	20	theme	intestinal	439:448	arg1	inflammation					450:461	intestinal inflammation	439:461	intestinal inflammation	439:461	In the current study, the functional roles and involved mechanisms of intestinal epithelial Reg4 in intestinal inflammation were studied in healthy and inflamed states using human intestinal specimens, an intestinal conditional Reg4 knockout mouse (Reg4ΔIEC) model and dextran sulfate sodium (DSS)-induced colitis model.
30953001	4	21	dep	cytokines	821:829	arg1	necrosis					862:869	tumor necrosis factor-α 	856:879	tumor necrosis factor-α (TNF-α)	856:886	We showed that the elevated serum Reg4 in pediatric intestinal failure (IF) patients were positively correlated with the serum concentrations of proinflammatory cytokines interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α).
30953001	4	21	dep	cytokines	821:829	arg1	TNF-α					881:885	TNF-α	881:885	TNF-α	881:885	We showed that the elevated serum Reg4 in pediatric intestinal failure (IF) patients were positively correlated with the serum concentrations of proinflammatory cytokines interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α).
30953001	4	21	dep	cytokines	821:829	arg1	cytokines					821:829	proinflammatory cytokines	805:829	proinflammatory cytokines interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α)	805:886	We showed that the elevated serum Reg4 in pediatric intestinal failure (IF) patients were positively correlated with the serum concentrations of proinflammatory cytokines interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α).
30953001	4	21	dep	cytokines	821:829	arg1	IL-6					846:849	IL-6	846:849	IL-6	846:849	We showed that the elevated serum Reg4 in pediatric intestinal failure (IF) patients were positively correlated with the serum concentrations of proinflammatory cytokines interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α).
30953001	4	21	dep	cytokines	821:829	arg1	interleukin-6					831:843	interleukin-6	831:843	interleukin-6 (IL-6)	831:850	We showed that the elevated serum Reg4 in pediatric intestinal failure (IF) patients were positively correlated with the serum concentrations of proinflammatory cytokines interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α).
30953001	3	22	theme	epithelial	420:429	arg1	Reg4					431:434	intestinal epithelial Reg4	409:434	intestinal epithelial Reg4	409:434	In the current study, the functional roles and involved mechanisms of intestinal epithelial Reg4 in intestinal inflammation were studied in healthy and inflamed states using human intestinal specimens, an intestinal conditional Reg4 knockout mouse (Reg4ΔIEC) model and dextran sulfate sodium (DSS)-induced colitis model.
30953001	3	23	from	mechanisms	395:404	arg1	inflammation					450:461	intestinal inflammation	439:461	intestinal inflammation	439:461	In the current study, the functional roles and involved mechanisms of intestinal epithelial Reg4 in intestinal inflammation were studied in healthy and inflamed states using human intestinal specimens, an intestinal conditional Reg4 knockout mouse (Reg4ΔIEC) model and dextran sulfate sodium (DSS)-induced colitis model.
30953001	8	24	theme	colonic	1343:1349	arg1	epithelium					1351:1360	the colonic epithelium	1339:1360	the colonic epithelium	1339:1360	Reg4ΔIEC mice altered the colonic bacterial composition and reduced the bacteria adhere to the colonic epithelium.
30953001	8	25	theme	bacterial	1282:1290	arg1	composition					1292:1302	the colonic bacterial composition	1270:1302	the colonic bacterial composition	1270:1302	Reg4ΔIEC mice altered the colonic bacterial composition and reduced the bacteria adhere to the colonic epithelium.
30953001	2	26	theme	Reg4	294:297	arg1	unknown					330:336	unknown	330:336	unknown	330:336	However, the physiological function of Reg4 in the inflammation is largely unknown.
30953001	2	26	theme	Reg4	294:297	arg1	function					282:289	the physiological function	264:289	the physiological function of Reg4 in the inflammation	264:317	However, the physiological function of Reg4 in the inflammation is largely unknown.
30953001	9	27	theme	organoids	1422:1430	arg1	growth					1404:1409	the growth	1400:1409	the growth of colonic organoids	1400:1430	In vitro, Reg4 was showed to promote the growth of colonic organoids, and that this occurs through a mechanism involving activation of signal transducer and activator of transcription 3 (STAT3).
30953001	9	28	theme	transducer	1505:1514	arg1	activation					1484:1493	activation	1484:1493	activation of signal transducer and activator of transcription 3 (STAT3)	1484:1555	In vitro, Reg4 was showed to promote the growth of colonic organoids, and that this occurs through a mechanism involving activation of signal transducer and activator of transcription 3 (STAT3).
30953001	1	29	from	member	161:166	arg1	tract					201:205	the gastrointestinal tract	180:205	the gastrointestinal tract	180:205	The regenerating islet-derived family member 4 (Reg4) in the gastrointestinal tract is up-regulated during intestinal inflammation.
30953001	5	30	theme	crypt	931:935	arg1	protein					947:953	the crypt base Reg4 protein	927:953	the crypt base Reg4 protein	927:953	In inflamed intestine of IF patients, the crypt base Reg4 protein was increased and highly expressed towards the luminal face.
30953001	1	31	theme	gastrointestinal	184:199	arg1	tract					201:205	the gastrointestinal tract	180:205	the gastrointestinal tract	180:205	The regenerating islet-derived family member 4 (Reg4) in the gastrointestinal tract is up-regulated during intestinal inflammation.
30953001	5	32	theme	base	937:940	arg1	protein					947:953	the crypt base Reg4 protein	927:953	the crypt base Reg4 protein	927:953	In inflamed intestine of IF patients, the crypt base Reg4 protein was increased and highly expressed towards the luminal face.
30953001	8	33	theme	Reg4ΔIEC	1248:1255	arg1	mice					1257:1260	Reg4ΔIEC mice	1248:1260	Reg4ΔIEC mice	1248:1260	Reg4ΔIEC mice altered the colonic bacterial composition and reduced the bacteria adhere to the colonic epithelium.
30953001	6	34	theme	novel	1044:1048	arg1	target					1050:1055	a novel target	1042:1055	a novel target of activating transcription factor 2 (ATF2) that enhanced Reg4 expression during the intestinal inflammation	1042:1164	The Reg4 was indicated as a novel target of activating transcription factor 2 (ATF2) that enhanced Reg4 expression during the intestinal inflammation.
30953001	6	34	theme	novel	1044:1048	arg1	Reg4					1020:1023	The Reg4	1016:1023	The Reg4	1016:1023	The Reg4 was indicated as a novel target of activating transcription factor 2 (ATF2) that enhanced Reg4 expression during the intestinal inflammation.
30953001	0	35	theme	epithelial	25:34	arg1	Reg4					36:39	intestinal epithelial Reg4	14:39	intestinal epithelial Reg4	14:39	Deficiency in intestinal epithelial Reg4 ameliorates intestinal inflammation and alters the colonic bacterial composition.
30953001	4	36	theme	cytokines	821:829	arg1	concentrations					787:800	the serum concentrations	777:800	the serum concentrations of proinflammatory cytokines interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α)	777:886	We showed that the elevated serum Reg4 in pediatric intestinal failure (IF) patients were positively correlated with the serum concentrations of proinflammatory cytokines interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α).
30953001	9	37	theme	colonic	1414:1420	arg1	organoids					1422:1430	colonic organoids	1414:1430	colonic organoids	1414:1430	In vitro, Reg4 was showed to promote the growth of colonic organoids, and that this occurs through a mechanism involving activation of signal transducer and activator of transcription 3 (STAT3).
30953001	5	38	theme	Reg4	942:945	arg1	protein					947:953	the crypt base Reg4 protein	927:953	the crypt base Reg4 protein	927:953	In inflamed intestine of IF patients, the crypt base Reg4 protein was increased and highly expressed towards the luminal face.
30953001	0	39	theme	intestinal	14:23	arg1	Reg4					36:39	intestinal epithelial Reg4	14:39	intestinal epithelial Reg4	14:39	Deficiency in intestinal epithelial Reg4 ameliorates intestinal inflammation and alters the colonic bacterial composition.
30953001	4	40	theme	serum	781:785	arg1	concentrations					787:800	the serum concentrations	777:800	the serum concentrations of proinflammatory cytokines interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α)	777:886	We showed that the elevated serum Reg4 in pediatric intestinal failure (IF) patients were positively correlated with the serum concentrations of proinflammatory cytokines interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α).
30953001	3	41	theme	Reg4ΔIEC	588:595	arg1	specimens					530:538	human intestinal specimens	513:538	human intestinal specimens	513:538	In the current study, the functional roles and involved mechanisms of intestinal epithelial Reg4 in intestinal inflammation were studied in healthy and inflamed states using human intestinal specimens, an intestinal conditional Reg4 knockout mouse (Reg4ΔIEC) model and dextran sulfate sodium (DSS)-induced colitis model.
30953001	3	41	theme	Reg4ΔIEC	588:595	arg1	model					598:602	an intestinal conditional Reg4 knockout mouse (Reg4ΔIEC) model	541:602	an intestinal conditional Reg4 knockout mouse (Reg4ΔIEC) model	541:602	In the current study, the functional roles and involved mechanisms of intestinal epithelial Reg4 in intestinal inflammation were studied in healthy and inflamed states using human intestinal specimens, an intestinal conditional Reg4 knockout mouse (Reg4ΔIEC) model and dextran sulfate sodium (DSS)-induced colitis model.
30953001	3	42	theme	inflamed	491:498	arg1	states					500:505	healthy and inflamed states	479:505	healthy and inflamed states	479:505	In the current study, the functional roles and involved mechanisms of intestinal epithelial Reg4 in intestinal inflammation were studied in healthy and inflamed states using human intestinal specimens, an intestinal conditional Reg4 knockout mouse (Reg4ΔIEC) model and dextran sulfate sodium (DSS)-induced colitis model.
30953001	3	43	theme	Reg4	431:434	arg1	mechanisms					395:404	involved mechanisms	386:404	involved mechanisms	386:404	In the current study, the functional roles and involved mechanisms of intestinal epithelial Reg4 in intestinal inflammation were studied in healthy and inflamed states using human intestinal specimens, an intestinal conditional Reg4 knockout mouse (Reg4ΔIEC) model and dextran sulfate sodium (DSS)-induced colitis model.
30953001	3	43	theme	Reg4	431:434	arg1	roles					376:380	the functional roles	361:380	the functional roles	361:380	In the current study, the functional roles and involved mechanisms of intestinal epithelial Reg4 in intestinal inflammation were studied in healthy and inflamed states using human intestinal specimens, an intestinal conditional Reg4 knockout mouse (Reg4ΔIEC) model and dextran sulfate sodium (DSS)-induced colitis model.
30953001	3	44	theme	intestinal	519:528	arg1	model					653:657	dextran sulfate sodium (DSS)-induced colitis model	608:657	dextran sulfate sodium (DSS)-induced colitis model	608:657	In the current study, the functional roles and involved mechanisms of intestinal epithelial Reg4 in intestinal inflammation were studied in healthy and inflamed states using human intestinal specimens, an intestinal conditional Reg4 knockout mouse (Reg4ΔIEC) model and dextran sulfate sodium (DSS)-induced colitis model.
30953001	3	44	theme	intestinal	519:528	arg1	specimens					530:538	human intestinal specimens	513:538	human intestinal specimens	513:538	In the current study, the functional roles and involved mechanisms of intestinal epithelial Reg4 in intestinal inflammation were studied in healthy and inflamed states using human intestinal specimens, an intestinal conditional Reg4 knockout mouse (Reg4ΔIEC) model and dextran sulfate sodium (DSS)-induced colitis model.
30953001	3	44	theme	intestinal	519:528	arg1	model					598:602	an intestinal conditional Reg4 knockout mouse (Reg4ΔIEC) model	541:602	an intestinal conditional Reg4 knockout mouse (Reg4ΔIEC) model	541:602	In the current study, the functional roles and involved mechanisms of intestinal epithelial Reg4 in intestinal inflammation were studied in healthy and inflamed states using human intestinal specimens, an intestinal conditional Reg4 knockout mouse (Reg4ΔIEC) model and dextran sulfate sodium (DSS)-induced colitis model.
30953001	5	45	theme	luminal	1002:1008	arg1	face					1010:1013	the luminal face	998:1013	the luminal face	998:1013	In inflamed intestine of IF patients, the crypt base Reg4 protein was increased and highly expressed towards the luminal face.
30953001	2	46	from	function	282:289	arg1	inflammation					306:317	the inflammation	302:317	the inflammation	302:317	However, the physiological function of Reg4 in the inflammation is largely unknown.
30953001	6	47	theme	activating	1060:1069	arg1	factor					1085:1090	activating transcription factor 2	1060:1092	activating transcription factor 2 (ATF2)	1060:1099	The Reg4 was indicated as a novel target of activating transcription factor 2 (ATF2) that enhanced Reg4 expression during the intestinal inflammation.
30953001	6	47	theme	activating	1060:1069	arg1	ATF2					1095:1098	ATF2	1095:1098	ATF2	1095:1098	The Reg4 was indicated as a novel target of activating transcription factor 2 (ATF2) that enhanced Reg4 expression during the intestinal inflammation.
30953001	10	48	theme	intestinal-epithelial	1599:1619	arg1	deficiency					1626:1635	intestinal-epithelial Reg4 deficiency	1599:1635	intestinal-epithelial Reg4 deficiency protects against experimental colitis and mucosal injury via a mechanism involving alteration of bacterial homeostasis and STAT3 activation	1599:1775	In conclusion, our findings demonstrated intestinal-epithelial Reg4 deficiency protects against experimental colitis and mucosal injury via a mechanism involving alteration of bacterial homeostasis and STAT3 activation.
30953001	3	49	theme	colitis	645:651	arg1	model					653:657	dextran sulfate sodium (DSS)-induced colitis model	608:657	dextran sulfate sodium (DSS)-induced colitis model	608:657	In the current study, the functional roles and involved mechanisms of intestinal epithelial Reg4 in intestinal inflammation were studied in healthy and inflamed states using human intestinal specimens, an intestinal conditional Reg4 knockout mouse (Reg4ΔIEC) model and dextran sulfate sodium (DSS)-induced colitis model.
30953001	3	49	theme	colitis	645:651	arg1	specimens					530:538	human intestinal specimens	513:538	human intestinal specimens	513:538	In the current study, the functional roles and involved mechanisms of intestinal epithelial Reg4 in intestinal inflammation were studied in healthy and inflamed states using human intestinal specimens, an intestinal conditional Reg4 knockout mouse (Reg4ΔIEC) model and dextran sulfate sodium (DSS)-induced colitis model.
30953001	8	50	dep	bacteria	1320:1327	arg1	adhere					1329:1334	adhere	1329:1334	adhere to the colonic epithelium	1329:1360	Reg4ΔIEC mice altered the colonic bacterial composition and reduced the bacteria adhere to the colonic epithelium.
30953001	1	51	theme	intestinal	230:239	arg1	inflammation					241:252	intestinal inflammation	230:252	intestinal inflammation	230:252	The regenerating islet-derived family member 4 (Reg4) in the gastrointestinal tract is up-regulated during intestinal inflammation.
30953001	3	52	theme	intestinal	409:418	arg1	Reg4					431:434	intestinal epithelial Reg4	409:434	intestinal epithelial Reg4	409:434	In the current study, the functional roles and involved mechanisms of intestinal epithelial Reg4 in intestinal inflammation were studied in healthy and inflamed states using human intestinal specimens, an intestinal conditional Reg4 knockout mouse (Reg4ΔIEC) model and dextran sulfate sodium (DSS)-induced colitis model.
30953001	4	53	theme	elevated	679:686	arg1	Reg4					694:697	the elevated serum Reg4	675:697	the elevated serum Reg4 in pediatric intestinal failure (IF) patients	675:743	We showed that the elevated serum Reg4 in pediatric intestinal failure (IF) patients were positively correlated with the serum concentrations of proinflammatory cytokines interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α).
30953001	3	54	from	roles	376:380	arg1	inflammation					450:461	intestinal inflammation	439:461	intestinal inflammation	439:461	In the current study, the functional roles and involved mechanisms of intestinal epithelial Reg4 in intestinal inflammation were studied in healthy and inflamed states using human intestinal specimens, an intestinal conditional Reg4 knockout mouse (Reg4ΔIEC) model and dextran sulfate sodium (DSS)-induced colitis model.
30953001	1	55	theme	regenerating	127:138	arg1	member					161:166	The regenerating islet-derived family member 4	123:168	The regenerating islet-derived family member 4 (Reg4) in the gastrointestinal tract	123:205	The regenerating islet-derived family member 4 (Reg4) in the gastrointestinal tract is up-regulated during intestinal inflammation.
30953001	1	55	theme	regenerating	127:138	arg1	Reg4					171:174	Reg4	171:174	Reg4	171:174	The regenerating islet-derived family member 4 (Reg4) in the gastrointestinal tract is up-regulated during intestinal inflammation.
30953001	0	56	theme	intestinal	53:62	arg1	inflammation					64:75	intestinal inflammation	53:75	intestinal inflammation	53:75	Deficiency in intestinal epithelial Reg4 ameliorates intestinal inflammation and alters the colonic bacterial composition.
30953001	9	57	theme	activator	1520:1528	arg1	activation					1484:1493	activation	1484:1493	activation of signal transducer and activator of transcription 3 (STAT3)	1484:1555	In vitro, Reg4 was showed to promote the growth of colonic organoids, and that this occurs through a mechanism involving activation of signal transducer and activator of transcription 3 (STAT3).
30953001	10	58	theme	STAT3	1760:1764	arg1	activation					1766:1775	STAT3 activation	1760:1775	STAT3 activation	1760:1775	In conclusion, our findings demonstrated intestinal-epithelial Reg4 deficiency protects against experimental colitis and mucosal injury via a mechanism involving alteration of bacterial homeostasis and STAT3 activation.
30953001	7	59	theme	DSS-induced	1180:1190	arg1	colitis					1192:1198	the DSS-induced colitis	1176:1198	the DSS-induced colitis	1176:1198	In vivo, the DSS-induced colitis was significantly ameliorated in Reg4ΔIEC mice.
30953001	1	60	theme	islet-derived	140:152	arg1	member					161:166	The regenerating islet-derived family member 4	123:168	The regenerating islet-derived family member 4 (Reg4) in the gastrointestinal tract	123:205	The regenerating islet-derived family member 4 (Reg4) in the gastrointestinal tract is up-regulated during intestinal inflammation.
30953001	1	60	theme	islet-derived	140:152	arg1	Reg4					171:174	Reg4	171:174	Reg4	171:174	The regenerating islet-derived family member 4 (Reg4) in the gastrointestinal tract is up-regulated during intestinal inflammation.
30953001	10	61	theme	Reg4	1621:1624	arg1	deficiency					1626:1635	intestinal-epithelial Reg4 deficiency	1599:1635	intestinal-epithelial Reg4 deficiency protects against experimental colitis and mucosal injury via a mechanism involving alteration of bacterial homeostasis and STAT3 activation	1599:1775	In conclusion, our findings demonstrated intestinal-epithelial Reg4 deficiency protects against experimental colitis and mucosal injury via a mechanism involving alteration of bacterial homeostasis and STAT3 activation.
30953001	4	62	theme	proinflammatory	805:819	arg1	necrosis					862:869	tumor necrosis factor-α 	856:879	tumor necrosis factor-α (TNF-α)	856:886	We showed that the elevated serum Reg4 in pediatric intestinal failure (IF) patients were positively correlated with the serum concentrations of proinflammatory cytokines interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α).
30953001	4	62	theme	proinflammatory	805:819	arg1	cytokines					821:829	proinflammatory cytokines	805:829	proinflammatory cytokines interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α)	805:886	We showed that the elevated serum Reg4 in pediatric intestinal failure (IF) patients were positively correlated with the serum concentrations of proinflammatory cytokines interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α).
30953001	4	62	theme	proinflammatory	805:819	arg1	interleukin-6					831:843	interleukin-6	831:843	interleukin-6 (IL-6)	831:850	We showed that the elevated serum Reg4 in pediatric intestinal failure (IF) patients were positively correlated with the serum concentrations of proinflammatory cytokines interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α).
30953001	4	63	theme	failure	723:729	arg1	patients					736:743	pediatric intestinal failure (IF) patients	702:743	pediatric intestinal failure (IF) patients	702:743	We showed that the elevated serum Reg4 in pediatric intestinal failure (IF) patients were positively correlated with the serum concentrations of proinflammatory cytokines interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α).
30953001	9	64	theme	signal	1498:1503	arg1	STAT3					1550:1554	STAT3	1550:1554	STAT3	1550:1554	In vitro, Reg4 was showed to promote the growth of colonic organoids, and that this occurs through a mechanism involving activation of signal transducer and activator of transcription 3 (STAT3).
30953001	9	64	theme	signal	1498:1503	arg1	transducer					1505:1514	signal transducer	1498:1514	signal transducer	1498:1514	In vitro, Reg4 was showed to promote the growth of colonic organoids, and that this occurs through a mechanism involving activation of signal transducer and activator of transcription 3 (STAT3).
30953001	1	65	theme	family	154:159	arg1	member					161:166	The regenerating islet-derived family member 4	123:168	The regenerating islet-derived family member 4 (Reg4) in the gastrointestinal tract	123:205	The regenerating islet-derived family member 4 (Reg4) in the gastrointestinal tract is up-regulated during intestinal inflammation.
30953001	1	65	theme	family	154:159	arg1	Reg4					171:174	Reg4	171:174	Reg4	171:174	The regenerating islet-derived family member 4 (Reg4) in the gastrointestinal tract is up-regulated during intestinal inflammation.
30953001	10	66	theme	homeostasis	1744:1754	arg1	activation					1766:1775	STAT3 activation	1760:1775	STAT3 activation	1760:1775	In conclusion, our findings demonstrated intestinal-epithelial Reg4 deficiency protects against experimental colitis and mucosal injury via a mechanism involving alteration of bacterial homeostasis and STAT3 activation.
30953001	10	66	theme	homeostasis	1744:1754	arg1	alteration					1720:1729	alteration	1720:1729	alteration of bacterial homeostasis	1720:1754	In conclusion, our findings demonstrated intestinal-epithelial Reg4 deficiency protects against experimental colitis and mucosal injury via a mechanism involving alteration of bacterial homeostasis and STAT3 activation.
30953001	3	67	theme	Reg4	567:570	arg1	specimens					530:538	human intestinal specimens	513:538	human intestinal specimens	513:538	In the current study, the functional roles and involved mechanisms of intestinal epithelial Reg4 in intestinal inflammation were studied in healthy and inflamed states using human intestinal specimens, an intestinal conditional Reg4 knockout mouse (Reg4ΔIEC) model and dextran sulfate sodium (DSS)-induced colitis model.
30953001	3	67	theme	Reg4	567:570	arg1	model					598:602	an intestinal conditional Reg4 knockout mouse (Reg4ΔIEC) model	541:602	an intestinal conditional Reg4 knockout mouse (Reg4ΔIEC) model	541:602	In the current study, the functional roles and involved mechanisms of intestinal epithelial Reg4 in intestinal inflammation were studied in healthy and inflamed states using human intestinal specimens, an intestinal conditional Reg4 knockout mouse (Reg4ΔIEC) model and dextran sulfate sodium (DSS)-induced colitis model.
30953001	3	68	theme	-induced	636:643	arg1	model					653:657	dextran sulfate sodium (DSS)-induced colitis model	608:657	dextran sulfate sodium (DSS)-induced colitis model	608:657	In the current study, the functional roles and involved mechanisms of intestinal epithelial Reg4 in intestinal inflammation were studied in healthy and inflamed states using human intestinal specimens, an intestinal conditional Reg4 knockout mouse (Reg4ΔIEC) model and dextran sulfate sodium (DSS)-induced colitis model.
30953001	3	68	theme	-induced	636:643	arg1	specimens					530:538	human intestinal specimens	513:538	human intestinal specimens	513:538	In the current study, the functional roles and involved mechanisms of intestinal epithelial Reg4 in intestinal inflammation were studied in healthy and inflamed states using human intestinal specimens, an intestinal conditional Reg4 knockout mouse (Reg4ΔIEC) model and dextran sulfate sodium (DSS)-induced colitis model.
30953001	4	69	theme	serum	688:692	arg1	Reg4					694:697	the elevated serum Reg4	675:697	the elevated serum Reg4 in pediatric intestinal failure (IF) patients	675:743	We showed that the elevated serum Reg4 in pediatric intestinal failure (IF) patients were positively correlated with the serum concentrations of proinflammatory cytokines interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α).
30953001	3	70	theme	current	346:352	arg1	study					354:358	the current study	342:358	the current study	342:358	In the current study, the functional roles and involved mechanisms of intestinal epithelial Reg4 in intestinal inflammation were studied in healthy and inflamed states using human intestinal specimens, an intestinal conditional Reg4 knockout mouse (Reg4ΔIEC) model and dextran sulfate sodium (DSS)-induced colitis model.
30953001	3	71	theme	healthy	479:485	arg1	states					500:505	healthy and inflamed states	479:505	healthy and inflamed states	479:505	In the current study, the functional roles and involved mechanisms of intestinal epithelial Reg4 in intestinal inflammation were studied in healthy and inflamed states using human intestinal specimens, an intestinal conditional Reg4 knockout mouse (Reg4ΔIEC) model and dextran sulfate sodium (DSS)-induced colitis model.
30953001	2	72	theme	physiological	268:280	arg1	unknown					330:336	unknown	330:336	unknown	330:336	However, the physiological function of Reg4 in the inflammation is largely unknown.
30953001	2	72	theme	physiological	268:280	arg1	function					282:289	the physiological function	264:289	the physiological function of Reg4 in the inflammation	264:317	However, the physiological function of Reg4 in the inflammation is largely unknown.
30953001	5	73	theme	inflamed	892:899	arg1	intestine					901:909	inflamed intestine	892:909	inflamed intestine of IF patients	892:924	In inflamed intestine of IF patients, the crypt base Reg4 protein was increased and highly expressed towards the luminal face.
30953001	10	74	dep	deficiency	1626:1635	arg1	protects					1637:1644	protects	1637:1644	protects against experimental colitis and mucosal injury via a mechanism involving alteration of bacterial homeostasis and STAT3 activation	1637:1775	In conclusion, our findings demonstrated intestinal-epithelial Reg4 deficiency protects against experimental colitis and mucosal injury via a mechanism involving alteration of bacterial homeostasis and STAT3 activation.
30953001	3	75	theme	involved	386:393	arg1	mechanisms					395:404	involved mechanisms	386:404	involved mechanisms	386:404	In the current study, the functional roles and involved mechanisms of intestinal epithelial Reg4 in intestinal inflammation were studied in healthy and inflamed states using human intestinal specimens, an intestinal conditional Reg4 knockout mouse (Reg4ΔIEC) model and dextran sulfate sodium (DSS)-induced colitis model.
30953001	9	76	theme	transcription	1533:1545	arg1	STAT3					1550:1554	STAT3	1550:1554	STAT3	1550:1554	In vitro, Reg4 was showed to promote the growth of colonic organoids, and that this occurs through a mechanism involving activation of signal transducer and activator of transcription 3 (STAT3).
30953001	9	76	theme	transcription	1533:1545	arg1	activator					1520:1528	activator	1520:1528	activator	1520:1528	In vitro, Reg4 was showed to promote the growth of colonic organoids, and that this occurs through a mechanism involving activation of signal transducer and activator of transcription 3 (STAT3).
30953001	9	76	theme	transcription	1533:1545	arg1	transducer					1505:1514	signal transducer	1498:1514	signal transducer	1498:1514	In vitro, Reg4 was showed to promote the growth of colonic organoids, and that this occurs through a mechanism involving activation of signal transducer and activator of transcription 3 (STAT3).
30953001	3	77	theme	intestinal	544:553	arg1	specimens					530:538	human intestinal specimens	513:538	human intestinal specimens	513:538	In the current study, the functional roles and involved mechanisms of intestinal epithelial Reg4 in intestinal inflammation were studied in healthy and inflamed states using human intestinal specimens, an intestinal conditional Reg4 knockout mouse (Reg4ΔIEC) model and dextran sulfate sodium (DSS)-induced colitis model.
30953001	3	77	theme	intestinal	544:553	arg1	model					598:602	an intestinal conditional Reg4 knockout mouse (Reg4ΔIEC) model	541:602	an intestinal conditional Reg4 knockout mouse (Reg4ΔIEC) model	541:602	In the current study, the functional roles and involved mechanisms of intestinal epithelial Reg4 in intestinal inflammation were studied in healthy and inflamed states using human intestinal specimens, an intestinal conditional Reg4 knockout mouse (Reg4ΔIEC) model and dextran sulfate sodium (DSS)-induced colitis model.
30953001	8	78	theme	colonic	1274:1280	arg1	composition					1292:1302	the colonic bacterial composition	1270:1302	the colonic bacterial composition	1270:1302	Reg4ΔIEC mice altered the colonic bacterial composition and reduced the bacteria adhere to the colonic epithelium.
30953001	4	79	theme	intestinal	712:721	arg1	failure					723:729	intestinal failure	712:729	pediatric intestinal failure (IF) patients	702:743	We showed that the elevated serum Reg4 in pediatric intestinal failure (IF) patients were positively correlated with the serum concentrations of proinflammatory cytokines interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α).
31693914	6	0	from	alterations	999:1009	arg1	coordination					1020:1031	motor coordination	1014:1031	motor coordination	1014:1031	The treatment with HypEO-βCD produced a longer-lasting anti-hyperalgesic effect compared to HypEO, without alterations in motor coordination or myorelaxant effects.
31693914	6	0	from	alterations	999:1009	arg1	effects					1048:1054	myorelaxant effects	1036:1054	myorelaxant effects	1036:1054	The treatment with HypEO-βCD produced a longer-lasting anti-hyperalgesic effect compared to HypEO, without alterations in motor coordination or myorelaxant effects.
31693914	9	1	theme	pain-inhibitory	1431:1445	arg1	pathway					1447:1453	the central pain-inhibitory pathway	1419:1453	the central pain-inhibitory pathway	1419:1453	We demonstrated that the anti-hyperalgesic effect produced by HypEO was improved after complexation with β-CD and this seems to be related to the central pain-inhibitory pathway, suggesting the possible use of E. fruticosa for chronic pain management.
31693914	8	2	from	decrease	1210:1217	arg1	expression					1234:1243	Fos protein expression	1222:1243	Fos protein expression in the spinal cord (p < 0.001)	1222:1274	Immunofluorescence assay demonstrated a decrease in Fos protein expression in the spinal cord (p < 0.001).
31693914	7	3	theme	anti-hyperalgesic	1110:1126	arg1	effect					1128:1133	a significant anti-hyperalgesic effect	1096:1133	a significant anti-hyperalgesic effect	1096:1133	Moreover, HypEO and HypEO-βCD produced a significant anti-hyperalgesic effect over 7 consecutive treatment days.
31693914	9	4	theme	central	1423:1429	arg1	pathway					1447:1453	the central pain-inhibitory pathway	1419:1453	the central pain-inhibitory pathway	1419:1453	We demonstrated that the anti-hyperalgesic effect produced by HypEO was improved after complexation with β-CD and this seems to be related to the central pain-inhibitory pathway, suggesting the possible use of E. fruticosa for chronic pain management.
31693914	2	5	theme	pain	521:524	arg1	model					533:537	a chronic widespread non-inflammatory muscle pain animal model	476:537	a chronic widespread non-inflammatory muscle pain animal model (a mice fibromyalgia-like model, FM)	476:574	We aimed to evaluate the anti-hyperalgesic effect of essential oil obtained from E. fruticosa (HypEO) complexed with βCD (HypEO-βCD) in a chronic widespread non-inflammatory muscle pain animal model (a mice fibromyalgia-like model, FM).
31693914	7	6	theme	significant	1098:1108	arg1	effect					1128:1133	a significant anti-hyperalgesic effect	1096:1133	a significant anti-hyperalgesic effect	1096:1133	Moreover, HypEO and HypEO-βCD produced a significant anti-hyperalgesic effect over 7 consecutive treatment days.
31693914	1	7	theme	important	260:268	arg1	herb					289:292	an important aromatic medicinal herb	257:292	an important aromatic medicinal herb used in folk medicine in northeastern Brazil	257:337	Eplingiella fruticosa (Lamiaceae), formally known as Hyptis fruticosa, is an important aromatic medicinal herb used in folk medicine in northeastern Brazil.
31693914	1	7	theme	important	260:268	arg1	fruticosa					195:203	Eplingiella fruticosa	183:203	Eplingiella fruticosa (Lamiaceae)	183:215	Eplingiella fruticosa (Lamiaceae), formally known as Hyptis fruticosa, is an important aromatic medicinal herb used in folk medicine in northeastern Brazil.
31693914	0	8	theme	anti-hyperalgesic	91:107	arg1	effect					109:114	its anti-hyperalgesic effect	87:114	its anti-hyperalgesic effect	87:114	Eplingiella fruticosa (Lamiaceae) essential oil complexed with β-cyclodextrin improves its anti-hyperalgesic effect in a chronic widespread non-inflammatory muscle pain animal model.
31693914	2	9	from	βCD	457:459	arg1	model					533:537	a chronic widespread non-inflammatory muscle pain animal model	476:537	a chronic widespread non-inflammatory muscle pain animal model (a mice fibromyalgia-like model, FM)	476:574	We aimed to evaluate the anti-hyperalgesic effect of essential oil obtained from E. fruticosa (HypEO) complexed with βCD (HypEO-βCD) in a chronic widespread non-inflammatory muscle pain animal model (a mice fibromyalgia-like model, FM).
31693914	1	10	from	medicine	307:314	arg1	Brazil					332:337	Brazil	332:337	Brazil	332:337	Eplingiella fruticosa (Lamiaceae), formally known as Hyptis fruticosa, is an important aromatic medicinal herb used in folk medicine in northeastern Brazil.
31693914	1	11	theme	aromatic	270:277	arg1	herb					289:292	an important aromatic medicinal herb	257:292	an important aromatic medicinal herb used in folk medicine in northeastern Brazil	257:337	Eplingiella fruticosa (Lamiaceae), formally known as Hyptis fruticosa, is an important aromatic medicinal herb used in folk medicine in northeastern Brazil.
31693914	1	11	theme	aromatic	270:277	arg1	fruticosa					195:203	Eplingiella fruticosa	183:203	Eplingiella fruticosa (Lamiaceae)	183:215	Eplingiella fruticosa (Lamiaceae), formally known as Hyptis fruticosa, is an important aromatic medicinal herb used in folk medicine in northeastern Brazil.
31693914	2	12	theme	non-inflammatory	497:512	arg1	model					533:537	a chronic widespread non-inflammatory muscle pain animal model	476:537	a chronic widespread non-inflammatory muscle pain animal model (a mice fibromyalgia-like model, FM)	476:574	We aimed to evaluate the anti-hyperalgesic effect of essential oil obtained from E. fruticosa (HypEO) complexed with βCD (HypEO-βCD) in a chronic widespread non-inflammatory muscle pain animal model (a mice fibromyalgia-like model, FM).
31693914	1	13	theme	Eplingiella	183:193	arg1	Lamiaceae					206:214	Lamiaceae	206:214	Lamiaceae	206:214	Eplingiella fruticosa (Lamiaceae), formally known as Hyptis fruticosa, is an important aromatic medicinal herb used in folk medicine in northeastern Brazil.
31693914	1	13	theme	Eplingiella	183:193	arg1	fruticosa					195:203	Eplingiella fruticosa	183:203	Eplingiella fruticosa (Lamiaceae)	183:215	Eplingiella fruticosa (Lamiaceae), formally known as Hyptis fruticosa, is an important aromatic medicinal herb used in folk medicine in northeastern Brazil.
31693914	1	13	theme	Eplingiella	183:193	arg1	herb					289:292	an important aromatic medicinal herb	257:292	an important aromatic medicinal herb used in folk medicine in northeastern Brazil	257:337	Eplingiella fruticosa (Lamiaceae), formally known as Hyptis fruticosa, is an important aromatic medicinal herb used in folk medicine in northeastern Brazil.
31693914	2	14	theme	widespread	486:495	arg1	model					533:537	a chronic widespread non-inflammatory muscle pain animal model	476:537	a chronic widespread non-inflammatory muscle pain animal model (a mice fibromyalgia-like model, FM)	476:574	We aimed to evaluate the anti-hyperalgesic effect of essential oil obtained from E. fruticosa (HypEO) complexed with βCD (HypEO-βCD) in a chronic widespread non-inflammatory muscle pain animal model (a mice fibromyalgia-like model, FM).
31693914	8	15	from	expression	1234:1243	arg1	p < 0.001					1265:1273	p < 0.001	1265:1273	p < 0.001	1265:1273	Immunofluorescence assay demonstrated a decrease in Fos protein expression in the spinal cord (p < 0.001).
31693914	8	15	from	expression	1234:1243	arg1	cord					1259:1262	the spinal cord	1248:1262	the spinal cord (p < 0.001)	1248:1274	Immunofluorescence assay demonstrated a decrease in Fos protein expression in the spinal cord (p < 0.001).
31693914	7	16	theme	treatment	1154:1162	arg1	days					1164:1167	7 consecutive treatment days	1140:1167	7 consecutive treatment days	1140:1167	Moreover, HypEO and HypEO-βCD produced a significant anti-hyperalgesic effect over 7 consecutive treatment days.
31693914	9	17	with	complexation	1364:1375	arg1	β-CD					1382:1385	β-CD	1382:1385	β-CD	1382:1385	We demonstrated that the anti-hyperalgesic effect produced by HypEO was improved after complexation with β-CD and this seems to be related to the central pain-inhibitory pathway, suggesting the possible use of E. fruticosa for chronic pain management.
31693914	2	18	theme	essential	393:401	arg1	oil					403:405	essential oil	393:405	essential oil obtained from E. fruticosa (HypEO) complexed with βCD (HypEO-βCD) in a chronic widespread non-inflammatory muscle pain animal model (a mice fibromyalgia-like model, FM)	393:574	We aimed to evaluate the anti-hyperalgesic effect of essential oil obtained from E. fruticosa (HypEO) complexed with βCD (HypEO-βCD) in a chronic widespread non-inflammatory muscle pain animal model (a mice fibromyalgia-like model, FM).
31693914	0	19	theme	fruticosa	12:20	arg1	oil					44:46	Eplingiella fruticosa (Lamiaceae) essential oil	0:46	Eplingiella fruticosa (Lamiaceae) essential oil complexed with β-cyclodextrin	0:76	Eplingiella fruticosa (Lamiaceae) essential oil complexed with β-cyclodextrin improves its anti-hyperalgesic effect in a chronic widespread non-inflammatory muscle pain animal model.
31693914	0	20	theme	pain	164:167	arg1	model					176:180	a chronic widespread non-inflammatory muscle pain animal model	119:180	a chronic widespread non-inflammatory muscle pain animal model	119:180	Eplingiella fruticosa (Lamiaceae) essential oil complexed with β-cyclodextrin improves its anti-hyperalgesic effect in a chronic widespread non-inflammatory muscle pain animal model.
31693914	0	21	theme	Eplingiella	0:10	arg1	oil					44:46	Eplingiella fruticosa (Lamiaceae) essential oil	0:46	Eplingiella fruticosa (Lamiaceae) essential oil complexed with β-cyclodextrin	0:76	Eplingiella fruticosa (Lamiaceae) essential oil complexed with β-cyclodextrin improves its anti-hyperalgesic effect in a chronic widespread non-inflammatory muscle pain animal model.
31693914	1	22	theme	medicinal	279:287	arg1	herb					289:292	an important aromatic medicinal herb	257:292	an important aromatic medicinal herb used in folk medicine in northeastern Brazil	257:337	Eplingiella fruticosa (Lamiaceae), formally known as Hyptis fruticosa, is an important aromatic medicinal herb used in folk medicine in northeastern Brazil.
31693914	1	22	theme	medicinal	279:287	arg1	fruticosa					195:203	Eplingiella fruticosa	183:203	Eplingiella fruticosa (Lamiaceae)	183:215	Eplingiella fruticosa (Lamiaceae), formally known as Hyptis fruticosa, is an important aromatic medicinal herb used in folk medicine in northeastern Brazil.
31693914	6	23	with	treatment	896:904	arg1	HypEO-βCD					911:919	HypEO-βCD	911:919	HypEO-βCD	911:919	The treatment with HypEO-βCD produced a longer-lasting anti-hyperalgesic effect compared to HypEO, without alterations in motor coordination or myorelaxant effects.
31693914	5	24	theme	other	846:850	arg1	compounds					852:860	21 other compounds	843:860	21 other compounds	843:860	(E)-caryophyllene, bicyclogermacrene, 1,8-cineole, α-pinene, β-pinene and 21 other compounds were identified in the HypEO.
31693914	0	25	theme	Lamiaceae	23:31	arg1	oil					44:46	Eplingiella fruticosa (Lamiaceae) essential oil	0:46	Eplingiella fruticosa (Lamiaceae) essential oil complexed with β-cyclodextrin	0:76	Eplingiella fruticosa (Lamiaceae) essential oil complexed with β-cyclodextrin improves its anti-hyperalgesic effect in a chronic widespread non-inflammatory muscle pain animal model.
31693914	6	26	theme	anti-hyperalgesic	947:963	arg1	effect					965:970	a longer-lasting anti-hyperalgesic effect	930:970	a longer-lasting anti-hyperalgesic effect	930:970	The treatment with HypEO-βCD produced a longer-lasting anti-hyperalgesic effect compared to HypEO, without alterations in motor coordination or myorelaxant effects.
31693914	2	27	theme	a	540:540	arg1	FM					572:573	FM	572:573	FM	572:573	We aimed to evaluate the anti-hyperalgesic effect of essential oil obtained from E. fruticosa (HypEO) complexed with βCD (HypEO-βCD) in a chronic widespread non-inflammatory muscle pain animal model (a mice fibromyalgia-like model, FM).
31693914	2	27	theme	a	540:540	arg1	mice					542:545	a mice	540:545	a mice	540:545	We aimed to evaluate the anti-hyperalgesic effect of essential oil obtained from E. fruticosa (HypEO) complexed with βCD (HypEO-βCD) in a chronic widespread non-inflammatory muscle pain animal model (a mice fibromyalgia-like model, FM).
31693914	6	28	theme	longer-lasting	932:945	arg1	effect					965:970	a longer-lasting anti-hyperalgesic effect	930:970	a longer-lasting anti-hyperalgesic effect	930:970	The treatment with HypEO-βCD produced a longer-lasting anti-hyperalgesic effect compared to HypEO, without alterations in motor coordination or myorelaxant effects.
31693914	0	29	theme	chronic	121:127	arg1	model					176:180	a chronic widespread non-inflammatory muscle pain animal model	119:180	a chronic widespread non-inflammatory muscle pain animal model	119:180	Eplingiella fruticosa (Lamiaceae) essential oil complexed with β-cyclodextrin improves its anti-hyperalgesic effect in a chronic widespread non-inflammatory muscle pain animal model.
31693914	4	30	theme	spinal	721:726	arg1	cord					728:731	the spinal cord	717:731	the spinal cord	717:731	Moreover, Fos protein expression in the spinal cord was assessed by immunofluorescence.
31693914	8	31	theme	Immunofluorescence	1170:1187	arg1	assay					1189:1193	Immunofluorescence assay	1170:1193	Immunofluorescence assay	1170:1193	Immunofluorescence assay demonstrated a decrease in Fos protein expression in the spinal cord (p < 0.001).
31693914	6	32	theme	myorelaxant	1036:1046	arg1	effects					1048:1054	myorelaxant effects	1036:1054	myorelaxant effects	1036:1054	The treatment with HypEO-βCD produced a longer-lasting anti-hyperalgesic effect compared to HypEO, without alterations in motor coordination or myorelaxant effects.
31693914	0	33	theme	essential	34:42	arg1	oil					44:46	Eplingiella fruticosa (Lamiaceae) essential oil	0:46	Eplingiella fruticosa (Lamiaceae) essential oil complexed with β-cyclodextrin	0:76	Eplingiella fruticosa (Lamiaceae) essential oil complexed with β-cyclodextrin improves its anti-hyperalgesic effect in a chronic widespread non-inflammatory muscle pain animal model.
31693914	0	34	theme	muscle	157:162	arg1	model					176:180	a chronic widespread non-inflammatory muscle pain animal model	119:180	a chronic widespread non-inflammatory muscle pain animal model	119:180	Eplingiella fruticosa (Lamiaceae) essential oil complexed with β-cyclodextrin improves its anti-hyperalgesic effect in a chronic widespread non-inflammatory muscle pain animal model.
31693914	9	35	theme	possible	1471:1478	arg1	use					1480:1482	the possible use	1467:1482	the possible use of E. fruticosa for chronic pain management	1467:1526	We demonstrated that the anti-hyperalgesic effect produced by HypEO was improved after complexation with β-CD and this seems to be related to the central pain-inhibitory pathway, suggesting the possible use of E. fruticosa for chronic pain management.
31693914	2	36	theme	oil	403:405	arg1	effect					383:388	the anti-hyperalgesic effect	361:388	the anti-hyperalgesic effect of essential oil obtained from E. fruticosa (HypEO) complexed with βCD (HypEO-βCD) in a chronic widespread non-inflammatory muscle pain animal model (a mice fibromyalgia-like model, FM)	361:574	We aimed to evaluate the anti-hyperalgesic effect of essential oil obtained from E. fruticosa (HypEO) complexed with βCD (HypEO-βCD) in a chronic widespread non-inflammatory muscle pain animal model (a mice fibromyalgia-like model, FM).
31693914	1	37	theme	folk	302:305	arg1	medicine					307:314	folk medicine	302:314	folk medicine in northeastern Brazil	302:337	Eplingiella fruticosa (Lamiaceae), formally known as Hyptis fruticosa, is an important aromatic medicinal herb used in folk medicine in northeastern Brazil.
31693914	2	38	theme	animal	526:531	arg1	model					533:537	a chronic widespread non-inflammatory muscle pain animal model	476:537	a chronic widespread non-inflammatory muscle pain animal model (a mice fibromyalgia-like model, FM)	476:574	We aimed to evaluate the anti-hyperalgesic effect of essential oil obtained from E. fruticosa (HypEO) complexed with βCD (HypEO-βCD) in a chronic widespread non-inflammatory muscle pain animal model (a mice fibromyalgia-like model, FM).
31693914	3	39	theme	hydro	604:608	arg1	distillation					610:621	hydro distillation	604:621	hydro distillation	604:621	The HypEO was extracted by hydro distillation and its chemical composition was determined by GC-MS/FID.
31693914	8	40	theme	Fos	1222:1224	arg1	expression					1234:1243	Fos protein expression	1222:1243	Fos protein expression in the spinal cord (p < 0.001)	1222:1274	Immunofluorescence assay demonstrated a decrease in Fos protein expression in the spinal cord (p < 0.001).
31693914	9	41	theme	fruticosa	1490:1498	arg1	use					1480:1482	the possible use	1467:1482	the possible use of E. fruticosa for chronic pain management	1467:1526	We demonstrated that the anti-hyperalgesic effect produced by HypEO was improved after complexation with β-CD and this seems to be related to the central pain-inhibitory pathway, suggesting the possible use of E. fruticosa for chronic pain management.
31693914	2	42	dep	model	533:537	arg1	FM					572:573	FM	572:573	FM	572:573	We aimed to evaluate the anti-hyperalgesic effect of essential oil obtained from E. fruticosa (HypEO) complexed with βCD (HypEO-βCD) in a chronic widespread non-inflammatory muscle pain animal model (a mice fibromyalgia-like model, FM).
31693914	2	42	dep	model	533:537	arg1	mice					542:545	a mice	540:545	a mice	540:545	We aimed to evaluate the anti-hyperalgesic effect of essential oil obtained from E. fruticosa (HypEO) complexed with βCD (HypEO-βCD) in a chronic widespread non-inflammatory muscle pain animal model (a mice fibromyalgia-like model, FM).
31693914	0	43	theme	animal	169:174	arg1	model					176:180	a chronic widespread non-inflammatory muscle pain animal model	119:180	a chronic widespread non-inflammatory muscle pain animal model	119:180	Eplingiella fruticosa (Lamiaceae) essential oil complexed with β-cyclodextrin improves its anti-hyperalgesic effect in a chronic widespread non-inflammatory muscle pain animal model.
31693914	4	44	theme	Fos	691:693	arg1	expression					703:712	Fos protein expression	691:712	Fos protein expression in the spinal cord	691:731	Moreover, Fos protein expression in the spinal cord was assessed by immunofluorescence.
31693914	0	45	theme	non-inflammatory	140:155	arg1	model					176:180	a chronic widespread non-inflammatory muscle pain animal model	119:180	a chronic widespread non-inflammatory muscle pain animal model	119:180	Eplingiella fruticosa (Lamiaceae) essential oil complexed with β-cyclodextrin improves its anti-hyperalgesic effect in a chronic widespread non-inflammatory muscle pain animal model.
31693914	0	46	theme	widespread	129:138	arg1	model					176:180	a chronic widespread non-inflammatory muscle pain animal model	119:180	a chronic widespread non-inflammatory muscle pain animal model	119:180	Eplingiella fruticosa (Lamiaceae) essential oil complexed with β-cyclodextrin improves its anti-hyperalgesic effect in a chronic widespread non-inflammatory muscle pain animal model.
31693914	8	47	theme	protein	1226:1232	arg1	expression					1234:1243	Fos protein expression	1222:1243	Fos protein expression in the spinal cord (p < 0.001)	1222:1274	Immunofluorescence assay demonstrated a decrease in Fos protein expression in the spinal cord (p < 0.001).
31693914	9	48	theme	chronic	1504:1510	arg1	management					1517:1526	chronic pain management	1504:1526	chronic pain management	1504:1526	We demonstrated that the anti-hyperalgesic effect produced by HypEO was improved after complexation with β-CD and this seems to be related to the central pain-inhibitory pathway, suggesting the possible use of E. fruticosa for chronic pain management.
31693914	4	49	from	expression	703:712	arg1	cord					728:731	the spinal cord	717:731	the spinal cord	717:731	Moreover, Fos protein expression in the spinal cord was assessed by immunofluorescence.
31693914	2	50	theme	fibromyalgia-like	547:563	arg1	model					565:569	fibromyalgia-like model	547:569	fibromyalgia-like model	547:569	We aimed to evaluate the anti-hyperalgesic effect of essential oil obtained from E. fruticosa (HypEO) complexed with βCD (HypEO-βCD) in a chronic widespread non-inflammatory muscle pain animal model (a mice fibromyalgia-like model, FM).
31693914	7	51	theme	consecutive	1142:1152	arg1	days					1164:1167	7 consecutive treatment days	1140:1167	7 consecutive treatment days	1140:1167	Moreover, HypEO and HypEO-βCD produced a significant anti-hyperalgesic effect over 7 consecutive treatment days.
31693914	9	52	theme	pain	1512:1515	arg1	management					1517:1526	chronic pain management	1504:1526	chronic pain management	1504:1526	We demonstrated that the anti-hyperalgesic effect produced by HypEO was improved after complexation with β-CD and this seems to be related to the central pain-inhibitory pathway, suggesting the possible use of E. fruticosa for chronic pain management.
31693914	6	53	theme	motor	1014:1018	arg1	coordination					1020:1031	motor coordination	1014:1031	motor coordination	1014:1031	The treatment with HypEO-βCD produced a longer-lasting anti-hyperalgesic effect compared to HypEO, without alterations in motor coordination or myorelaxant effects.
31693914	3	54	theme	chemical	631:638	arg1	composition					640:650	its chemical composition	627:650	its chemical composition	627:650	The HypEO was extracted by hydro distillation and its chemical composition was determined by GC-MS/FID.
31693914	4	55	theme	protein	695:701	arg1	expression					703:712	Fos protein expression	691:712	Fos protein expression in the spinal cord	691:731	Moreover, Fos protein expression in the spinal cord was assessed by immunofluorescence.
31693914	1	56	theme	Hyptis	236:241	arg1	fruticosa					243:251	Hyptis fruticosa	236:251	Hyptis fruticosa	236:251	Eplingiella fruticosa (Lamiaceae), formally known as Hyptis fruticosa, is an important aromatic medicinal herb used in folk medicine in northeastern Brazil.
31693914	2	57	theme	chronic	478:484	arg1	model					533:537	a chronic widespread non-inflammatory muscle pain animal model	476:537	a chronic widespread non-inflammatory muscle pain animal model (a mice fibromyalgia-like model, FM)	476:574	We aimed to evaluate the anti-hyperalgesic effect of essential oil obtained from E. fruticosa (HypEO) complexed with βCD (HypEO-βCD) in a chronic widespread non-inflammatory muscle pain animal model (a mice fibromyalgia-like model, FM).
31693914	2	58	theme	anti-hyperalgesic	365:381	arg1	effect					383:388	the anti-hyperalgesic effect	361:388	the anti-hyperalgesic effect of essential oil obtained from E. fruticosa (HypEO) complexed with βCD (HypEO-βCD) in a chronic widespread non-inflammatory muscle pain animal model (a mice fibromyalgia-like model, FM)	361:574	We aimed to evaluate the anti-hyperalgesic effect of essential oil obtained from E. fruticosa (HypEO) complexed with βCD (HypEO-βCD) in a chronic widespread non-inflammatory muscle pain animal model (a mice fibromyalgia-like model, FM).
31693914	8	59	theme	spinal	1252:1257	arg1	p < 0.001					1265:1273	p < 0.001	1265:1273	p < 0.001	1265:1273	Immunofluorescence assay demonstrated a decrease in Fos protein expression in the spinal cord (p < 0.001).
31693914	8	59	theme	spinal	1252:1257	arg1	cord					1259:1262	the spinal cord	1248:1262	the spinal cord (p < 0.001)	1248:1274	Immunofluorescence assay demonstrated a decrease in Fos protein expression in the spinal cord (p < 0.001).
31693914	2	60	dep	mice	542:545	arg1	model					565:569	fibromyalgia-like model	547:569	fibromyalgia-like model	547:569	We aimed to evaluate the anti-hyperalgesic effect of essential oil obtained from E. fruticosa (HypEO) complexed with βCD (HypEO-βCD) in a chronic widespread non-inflammatory muscle pain animal model (a mice fibromyalgia-like model, FM).
31693914	9	61	theme	anti-hyperalgesic	1302:1318	arg1	effect					1320:1325	the anti-hyperalgesic effect	1298:1325	the anti-hyperalgesic effect produced by HypEO	1298:1343	We demonstrated that the anti-hyperalgesic effect produced by HypEO was improved after complexation with β-CD and this seems to be related to the central pain-inhibitory pathway, suggesting the possible use of E. fruticosa for chronic pain management.
31693914	2	62	theme	muscle	514:519	arg1	model					533:537	a chronic widespread non-inflammatory muscle pain animal model	476:537	a chronic widespread non-inflammatory muscle pain animal model (a mice fibromyalgia-like model, FM)	476:574	We aimed to evaluate the anti-hyperalgesic effect of essential oil obtained from E. fruticosa (HypEO) complexed with βCD (HypEO-βCD) in a chronic widespread non-inflammatory muscle pain animal model (a mice fibromyalgia-like model, FM).
31157612	5	0	theme	respiratory	603:613	arg1	feature					453:459	summed feature 3	446:461	summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c)	446:503	Cells contained summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0 and iso-C17 : 0 3-OH as its major cellular fatty acids and menaquinone-7 as the only respiratory quinone.
31157612	5	0	theme	respiratory	603:613	arg1	quinone					615:621	the only respiratory quinone	594:621	the only respiratory quinone	594:621	Cells contained summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0 and iso-C17 : 0 3-OH as its major cellular fatty acids and menaquinone-7 as the only respiratory quinone.
31157612	8	1	theme	highest	872:878	arg1	similarity					889:898	the highest sequence similarity	868:898	the highest sequence similarity (96.7 %) to Pedobacteragri JCM 15120T	868:936	On the basis 16S rRNA gene sequence comparison, strain JDX94T showed the highest sequence similarity (96.7 %) to Pedobacteragri JCM 15120T, followed by Pedobacteralluvionis DSM 19624T (96.3 %).
31157612	8	1	theme	highest	872:878	arg1	%					906:906	96.7 %	901:906	96.7 %	901:906	On the basis 16S rRNA gene sequence comparison, strain JDX94T showed the highest sequence similarity (96.7 %) to Pedobacteragri JCM 15120T, followed by Pedobacteralluvionis DSM 19624T (96.3 %).
31157612	8	2	theme	strain	847:852	arg1	JDX94T					854:859	strain JDX94T	847:859	strain JDX94T	847:859	On the basis 16S rRNA gene sequence comparison, strain JDX94T showed the highest sequence similarity (96.7 %) to Pedobacteragri JCM 15120T, followed by Pedobacteralluvionis DSM 19624T (96.3 %).
31157612	9	3	theme	strain	1087:1092	arg1	JDX94T					1094:1099	strain JDX94T	1087:1099	strain JDX94T	1087:1099	Furthermore, the average nucleotide identity and digital DNA-DNA hybridization values between strain JDX94T and related species of the genus Pedobacter were 74.6-79.2 % and 18.9-24.5 %, respectively.
31157612	4	4	with	4-37 °C	317:323	arg1	optimum					333:339	an optimum	330:339	an optimum at 28 °C	330:348	The strain displayed growth at 4-37 °C with an optimum at 28 °C, with 0-1.0 % (w/v) NaCl (optimum, 0%) and at pH 6.0-9.0 (optimum, pH 7.0-7.5).
31157612	9	5	theme	genus	1128:1132	arg1	Pedobacter					1134:1143	the genus Pedobacter	1124:1143	the genus Pedobacter	1124:1143	Furthermore, the average nucleotide identity and digital DNA-DNA hybridization values between strain JDX94T and related species of the genus Pedobacter were 74.6-79.2 % and 18.9-24.5 %, respectively.
31157612	4	6	theme	%	362:362	arg1	optimum					376:382	optimum	376:382	optimum	376:382	The strain displayed growth at 4-37 °C with an optimum at 28 °C, with 0-1.0 % (w/v) NaCl (optimum, 0%) and at pH 6.0-9.0 (optimum, pH 7.0-7.5).
31157612	4	6	theme	%	362:362	arg1	NaCl					370:373	0-1.0 % (w/v) NaCl	356:373	0-1.0 % (w/v) NaCl (optimum, 0%)	356:387	The strain displayed growth at 4-37 °C with an optimum at 28 °C, with 0-1.0 % (w/v) NaCl (optimum, 0%) and at pH 6.0-9.0 (optimum, pH 7.0-7.5).
31157612	9	7	theme	identity	1029:1036	arg1	values					1072:1077	the average nucleotide identity and digital DNA-DNA hybridization values	1006:1077	the average nucleotide identity and digital DNA-DNA hybridization values between strain JDX94T and related species of the genus Pedobacter	1006:1143	Furthermore, the average nucleotide identity and digital DNA-DNA hybridization values between strain JDX94T and related species of the genus Pedobacter were 74.6-79.2 % and 18.9-24.5 %, respectively.
31157612	9	7	theme	identity	1029:1036	arg1	%					1176:1176	74.6-79.2 % and 18.9-24.5 %	1150:1176	74.6-79.2 % and 18.9-24.5 %	1150:1176	Furthermore, the average nucleotide identity and digital DNA-DNA hybridization values between strain JDX94T and related species of the genus Pedobacter were 74.6-79.2 % and 18.9-24.5 %, respectively.
31157612	10	8	theme	name	1266:1269	arg1	sp					1291:1292	the name Pedobacterchinensis sp	1262:1292	the name Pedobacterchinensis sp	1262:1292	Based on the presented results, we propose a novel species for which the name Pedobacterchinensis sp.
31157612	2	9	theme	bacterial	98:106	arg1	JDX94T					116:121	JDX94T	116:121	JDX94T	116:121	A novel bacterial strain, JDX94T, was isolated from tundra soil sampled north of the Yellow River station, Arctic.
31157612	2	9	theme	bacterial	98:106	arg1	strain					108:113	A novel bacterial strain	90:113	A novel bacterial strain	90:113	A novel bacterial strain, JDX94T, was isolated from tundra soil sampled north of the Yellow River station, Arctic.
31157612	10	10	theme	presented	1206:1214	arg1	results					1216:1222	the presented results	1202:1222	the presented results	1202:1222	Based on the presented results, we propose a novel species for which the name Pedobacterchinensis sp.
31157612	6	11	theme	strain	651:656	arg1	JDX94T					658:663	strain JDX94T	651:663	strain JDX94T	651:663	The polar lipid profile of strain JDX94T consisted of phosphatidylethanolamine, two unidentified aminolipids and four unknown polar lipids.
31157612	8	12	theme	basis	806:810	arg1	comparison					835:844	the basis 16S rRNA gene sequence comparison	802:844	the basis 16S rRNA gene sequence comparison	802:844	On the basis 16S rRNA gene sequence comparison, strain JDX94T showed the highest sequence similarity (96.7 %) to Pedobacteragri JCM 15120T, followed by Pedobacteralluvionis DSM 19624T (96.3 %).
31157612	9	13	theme	Pedobacter	1134:1143	arg1	JDX94T					1094:1099	strain JDX94T	1087:1099	strain JDX94T	1087:1099	Furthermore, the average nucleotide identity and digital DNA-DNA hybridization values between strain JDX94T and related species of the genus Pedobacter were 74.6-79.2 % and 18.9-24.5 %, respectively.
31157612	9	13	theme	Pedobacter	1134:1143	arg1	species					1113:1119	related species	1105:1119	related species of the genus Pedobacter	1105:1143	Furthermore, the average nucleotide identity and digital DNA-DNA hybridization values between strain JDX94T and related species of the genus Pedobacter were 74.6-79.2 % and 18.9-24.5 %, respectively.
31157612	8	14	theme	sequence	880:887	arg1	similarity					889:898	the highest sequence similarity	868:898	the highest sequence similarity (96.7 %) to Pedobacteragri JCM 15120T	868:936	On the basis 16S rRNA gene sequence comparison, strain JDX94T showed the highest sequence similarity (96.7 %) to Pedobacteragri JCM 15120T, followed by Pedobacteralluvionis DSM 19624T (96.3 %).
31157612	8	14	theme	sequence	880:887	arg1	%					906:906	96.7 %	901:906	96.7 %	901:906	On the basis 16S rRNA gene sequence comparison, strain JDX94T showed the highest sequence similarity (96.7 %) to Pedobacteragri JCM 15120T, followed by Pedobacteralluvionis DSM 19624T (96.3 %).
31157612	4	15	theme	w/v	365:367	arg1	optimum					376:382	optimum	376:382	optimum	376:382	The strain displayed growth at 4-37 °C with an optimum at 28 °C, with 0-1.0 % (w/v) NaCl (optimum, 0%) and at pH 6.0-9.0 (optimum, pH 7.0-7.5).
31157612	4	15	theme	w/v	365:367	arg1	NaCl					370:373	0-1.0 % (w/v) NaCl	356:373	0-1.0 % (w/v) NaCl (optimum, 0%)	356:387	The strain displayed growth at 4-37 °C with an optimum at 28 °C, with 0-1.0 % (w/v) NaCl (optimum, 0%) and at pH 6.0-9.0 (optimum, pH 7.0-7.5).
31157612	9	16	theme	DNA-DNA	1050:1056	arg1	hybridization					1058:1070	digital DNA-DNA hybridization	1042:1070	digital DNA-DNA hybridization	1042:1070	Furthermore, the average nucleotide identity and digital DNA-DNA hybridization values between strain JDX94T and related species of the genus Pedobacter were 74.6-79.2 % and 18.9-24.5 %, respectively.
31157612	5	17	dep	feature	453:459	arg1	C16 					475:478	C16 	475:478	C16 	475:478	Cells contained summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0 and iso-C17 : 0 3-OH as its major cellular fatty acids and menaquinone-7 as the only respiratory quinone.
31157612	6	18	theme	polar	750:754	arg1	lipids					756:761	four unknown polar lipids	737:761	four unknown polar lipids	737:761	The polar lipid profile of strain JDX94T consisted of phosphatidylethanolamine, two unidentified aminolipids and four unknown polar lipids.
31157612	8	19	theme	sequence	826:833	arg1	comparison					835:844	the basis 16S rRNA gene sequence comparison	802:844	the basis 16S rRNA gene sequence comparison	802:844	On the basis 16S rRNA gene sequence comparison, strain JDX94T showed the highest sequence similarity (96.7 %) to Pedobacteragri JCM 15120T, followed by Pedobacteralluvionis DSM 19624T (96.3 %).
31157612	1	20	theme	Arctic	70:75	arg1	soil					84:87	Arctic tundra soil	70:87	Arctic tundra soil	70:87	nov., a cellulose-decomposing bacterium from Arctic tundra soil.
31157612	6	21	theme	unknown	742:748	arg1	lipids					756:761	four unknown polar lipids	737:761	four unknown polar lipids	737:761	The polar lipid profile of strain JDX94T consisted of phosphatidylethanolamine, two unidentified aminolipids and four unknown polar lipids.
31157612	6	22	theme	lipid	634:638	arg1	profile					640:646	The polar lipid profile	624:646	The polar lipid profile of strain JDX94T	624:663	The polar lipid profile of strain JDX94T consisted of phosphatidylethanolamine, two unidentified aminolipids and four unknown polar lipids.
31157612	1	23	theme	tundra	77:82	arg1	soil					84:87	Arctic tundra soil	70:87	Arctic tundra soil	70:87	nov., a cellulose-decomposing bacterium from Arctic tundra soil.
31157612	0	24	theme	chinensis	11:19	arg1	sp					21:22	Pedobacter chinensis sp	0:22	Pedobacter chinensis sp.	0:23	Pedobacter chinensis sp.
31157612	9	25	theme	 and	1161:1164	arg1	%					1176:1176	74.6-79.2 % and 18.9-24.5 %	1150:1176	74.6-79.2 % and 18.9-24.5 %	1150:1176	Furthermore, the average nucleotide identity and digital DNA-DNA hybridization values between strain JDX94T and related species of the genus Pedobacter were 74.6-79.2 % and 18.9-24.5 %, respectively.
31157612	9	25	theme	 and	1161:1164	arg1	values					1072:1077	the average nucleotide identity and digital DNA-DNA hybridization values	1006:1077	the average nucleotide identity and digital DNA-DNA hybridization values between strain JDX94T and related species of the genus Pedobacter	1006:1143	Furthermore, the average nucleotide identity and digital DNA-DNA hybridization values between strain JDX94T and related species of the genus Pedobacter were 74.6-79.2 % and 18.9-24.5 %, respectively.
31157612	6	26	theme	polar	628:632	arg1	profile					640:646	The polar lipid profile	624:646	The polar lipid profile of strain JDX94T	624:663	The polar lipid profile of strain JDX94T consisted of phosphatidylethanolamine, two unidentified aminolipids and four unknown polar lipids.
31157612	0	27	theme	Pedobacter	0:9	arg1	sp					21:22	Pedobacter chinensis sp	0:22	Pedobacter chinensis sp.	0:23	Pedobacter chinensis sp.
31157612	9	28	theme	18.9-24.5 	1166:1175	arg1	%					1176:1176	74.6-79.2 % and 18.9-24.5 %	1150:1176	74.6-79.2 % and 18.9-24.5 %	1150:1176	Furthermore, the average nucleotide identity and digital DNA-DNA hybridization values between strain JDX94T and related species of the genus Pedobacter were 74.6-79.2 % and 18.9-24.5 %, respectively.
31157612	9	28	theme	18.9-24.5 	1166:1175	arg1	values					1072:1077	the average nucleotide identity and digital DNA-DNA hybridization values	1006:1077	the average nucleotide identity and digital DNA-DNA hybridization values between strain JDX94T and related species of the genus Pedobacter	1006:1143	Furthermore, the average nucleotide identity and digital DNA-DNA hybridization values between strain JDX94T and related species of the genus Pedobacter were 74.6-79.2 % and 18.9-24.5 %, respectively.
31157612	10	29	theme	Pedobacterchinensis	1271:1289	arg1	sp					1291:1292	the name Pedobacterchinensis sp	1262:1292	the name Pedobacterchinensis sp	1262:1292	Based on the presented results, we propose a novel species for which the name Pedobacterchinensis sp.
31157612	9	30	theme	digital	1042:1048	arg1	hybridization					1058:1070	digital DNA-DNA hybridization	1042:1070	digital DNA-DNA hybridization	1042:1070	Furthermore, the average nucleotide identity and digital DNA-DNA hybridization values between strain JDX94T and related species of the genus Pedobacter were 74.6-79.2 % and 18.9-24.5 %, respectively.
31157612	8	31	theme	Pedobacteragri	912:925	arg1	15120T					931:936	Pedobacteragri JCM 15120T	912:936	Pedobacteragri JCM 15120T	912:936	On the basis 16S rRNA gene sequence comparison, strain JDX94T showed the highest sequence similarity (96.7 %) to Pedobacteragri JCM 15120T, followed by Pedobacteralluvionis DSM 19624T (96.3 %).
31157612	7	32	theme	37.5 mol	788:795	arg1	%					796:796	37.5 mol%	788:796	37.5 mol%	788:796	The DNA G+C content was 37.5 mol%.
31157612	7	32	theme	37.5 mol	788:795	arg1	G+C content					772:782	The DNA G+C content	764:782	The DNA G+C content	764:782	The DNA G+C content was 37.5 mol%.
31157612	4	33	from	28 °C	344:348	arg1	optimum					333:339	an optimum	330:339	an optimum at 28 °C	330:348	The strain displayed growth at 4-37 °C with an optimum at 28 °C, with 0-1.0 % (w/v) NaCl (optimum, 0%) and at pH 6.0-9.0 (optimum, pH 7.0-7.5).
31157612	6	34	theme	JDX94T	658:663	arg1	profile					640:646	The polar lipid profile	624:646	The polar lipid profile of strain JDX94T	624:663	The polar lipid profile of strain JDX94T consisted of phosphatidylethanolamine, two unidentified aminolipids and four unknown polar lipids.
31157612	5	35	theme	major	546:550	arg1	acids					567:571	its major cellular fatty acids	542:571	its major cellular fatty acids	542:571	Cells contained summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0 and iso-C17 : 0 3-OH as its major cellular fatty acids and menaquinone-7 as the only respiratory quinone.
31157612	5	35	theme	major	546:550	arg1	feature					453:459	summed feature 3	446:461	summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c)	446:503	Cells contained summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0 and iso-C17 : 0 3-OH as its major cellular fatty acids and menaquinone-7 as the only respiratory quinone.
31157612	9	36	theme	hybridization	1058:1070	arg1	values					1072:1077	the average nucleotide identity and digital DNA-DNA hybridization values	1006:1077	the average nucleotide identity and digital DNA-DNA hybridization values between strain JDX94T and related species of the genus Pedobacter	1006:1143	Furthermore, the average nucleotide identity and digital DNA-DNA hybridization values between strain JDX94T and related species of the genus Pedobacter were 74.6-79.2 % and 18.9-24.5 %, respectively.
31157612	9	36	theme	hybridization	1058:1070	arg1	%					1176:1176	74.6-79.2 % and 18.9-24.5 %	1150:1176	74.6-79.2 % and 18.9-24.5 %	1150:1176	Furthermore, the average nucleotide identity and digital DNA-DNA hybridization values between strain JDX94T and related species of the genus Pedobacter were 74.6-79.2 % and 18.9-24.5 %, respectively.
31157612	10	37	theme	novel	1238:1242	arg1	species					1244:1250	a novel species	1236:1250	a novel species for which the name Pedobacterchinensis sp	1236:1292	Based on the presented results, we propose a novel species for which the name Pedobacterchinensis sp.
31157612	8	38	theme	gene	821:824	arg1	comparison					835:844	the basis 16S rRNA gene sequence comparison	802:844	the basis 16S rRNA gene sequence comparison	802:844	On the basis 16S rRNA gene sequence comparison, strain JDX94T showed the highest sequence similarity (96.7 %) to Pedobacteragri JCM 15120T, followed by Pedobacteralluvionis DSM 19624T (96.3 %).
31157612	5	39	theme	cellular	552:559	arg1	acids					567:571	its major cellular fatty acids	542:571	its major cellular fatty acids	542:571	Cells contained summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0 and iso-C17 : 0 3-OH as its major cellular fatty acids and menaquinone-7 as the only respiratory quinone.
31157612	5	39	theme	cellular	552:559	arg1	feature					453:459	summed feature 3	446:461	summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c)	446:503	Cells contained summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0 and iso-C17 : 0 3-OH as its major cellular fatty acids and menaquinone-7 as the only respiratory quinone.
31157612	2	40	theme	sampled	154:160	arg1	north					162:166	tundra soil sampled north	142:166	tundra soil sampled north of the Yellow River station, Arctic	142:202	A novel bacterial strain, JDX94T, was isolated from tundra soil sampled north of the Yellow River station, Arctic.
31157612	11	41	theme	1H00335T=	1349:1357	arg1	62850T					1364:1369	=MCCC 1H00335T= KCTC 62850T	1343:1369	=MCCC 1H00335T= KCTC 62850T	1343:1369	nov. is suggested, with the type strain JDX94T (=MCCC 1H00335T= KCTC 62850T).
31157612	11	41	theme	1H00335T=	1349:1357	arg1	JDX94T					1335:1340	the type strain JDX94T	1319:1340	the type strain JDX94T (=MCCC 1H00335T= KCTC 62850T)	1319:1370	nov. is suggested, with the type strain JDX94T (=MCCC 1H00335T= KCTC 62850T).
31157612	11	42	theme	KCTC	1359:1362	arg1	62850T					1364:1369	=MCCC 1H00335T= KCTC 62850T	1343:1369	=MCCC 1H00335T= KCTC 62850T	1343:1369	nov. is suggested, with the type strain JDX94T (=MCCC 1H00335T= KCTC 62850T).
31157612	11	42	theme	KCTC	1359:1362	arg1	JDX94T					1335:1340	the type strain JDX94T	1319:1340	the type strain JDX94T (=MCCC 1H00335T= KCTC 62850T)	1319:1370	nov. is suggested, with the type strain JDX94T (=MCCC 1H00335T= KCTC 62850T).
31157612	5	43	dep	iso-C15 	506:513	arg1	3-OH					534:537	 0 3-OH	531:537	iso-C15 : 0 and iso-C17 : 0 3-OH	506:537	Cells contained summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0 and iso-C17 : 0 3-OH as its major cellular fatty acids and menaquinone-7 as the only respiratory quinone.
31157612	5	43	dep	iso-C15 	506:513	arg1	 0					515:516	 0	515:516	 0	515:516	Cells contained summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0 and iso-C17 : 0 3-OH as its major cellular fatty acids and menaquinone-7 as the only respiratory quinone.
31157612	5	43	dep	iso-C15 	506:513	arg1	iso-C17 					522:529	iso-C17 	522:529	iso-C17 	522:529	Cells contained summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0 and iso-C17 : 0 3-OH as its major cellular fatty acids and menaquinone-7 as the only respiratory quinone.
31157612	2	44	theme	novel	92:96	arg1	JDX94T					116:121	JDX94T	116:121	JDX94T	116:121	A novel bacterial strain, JDX94T, was isolated from tundra soil sampled north of the Yellow River station, Arctic.
31157612	2	44	theme	novel	92:96	arg1	strain					108:113	A novel bacterial strain	90:113	A novel bacterial strain	90:113	A novel bacterial strain, JDX94T, was isolated from tundra soil sampled north of the Yellow River station, Arctic.
31157612	5	45	theme	fatty	561:565	arg1	acids					567:571	its major cellular fatty acids	542:571	its major cellular fatty acids	542:571	Cells contained summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0 and iso-C17 : 0 3-OH as its major cellular fatty acids and menaquinone-7 as the only respiratory quinone.
31157612	5	45	theme	fatty	561:565	arg1	feature					453:459	summed feature 3	446:461	summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c)	446:503	Cells contained summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0 and iso-C17 : 0 3-OH as its major cellular fatty acids and menaquinone-7 as the only respiratory quinone.
31157612	2	46	theme	soil	149:152	arg1	north					162:166	tundra soil sampled north	142:166	tundra soil sampled north of the Yellow River station, Arctic	142:202	A novel bacterial strain, JDX94T, was isolated from tundra soil sampled north of the Yellow River station, Arctic.
31157612	9	47	theme	nucleotide	1018:1027	arg1	identity					1029:1036	average nucleotide identity	1010:1036	average nucleotide identity	1010:1036	Furthermore, the average nucleotide identity and digital DNA-DNA hybridization values between strain JDX94T and related species of the genus Pedobacter were 74.6-79.2 % and 18.9-24.5 %, respectively.
31157612	9	48	theme	average	1010:1016	arg1	identity					1029:1036	average nucleotide identity	1010:1036	average nucleotide identity	1010:1036	Furthermore, the average nucleotide identity and digital DNA-DNA hybridization values between strain JDX94T and related species of the genus Pedobacter were 74.6-79.2 % and 18.9-24.5 %, respectively.
31157612	2	49	theme	tundra	142:147	arg1	north					162:166	tundra soil sampled north	142:166	tundra soil sampled north of the Yellow River station, Arctic	142:202	A novel bacterial strain, JDX94T, was isolated from tundra soil sampled north of the Yellow River station, Arctic.
31157612	5	50	theme	summed	446:451	arg1	acids					567:571	its major cellular fatty acids	542:571	its major cellular fatty acids	542:571	Cells contained summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0 and iso-C17 : 0 3-OH as its major cellular fatty acids and menaquinone-7 as the only respiratory quinone.
31157612	5	50	theme	summed	446:451	arg1	iso-C15 					506:513	iso-C15 	506:513	iso-C15 : 0 and iso-C17 : 0 3-OH	506:537	Cells contained summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0 and iso-C17 : 0 3-OH as its major cellular fatty acids and menaquinone-7 as the only respiratory quinone.
31157612	5	50	theme	summed	446:451	arg1	feature					453:459	summed feature 3	446:461	summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c)	446:503	Cells contained summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0 and iso-C17 : 0 3-OH as its major cellular fatty acids and menaquinone-7 as the only respiratory quinone.
31157612	5	50	theme	summed	446:451	arg1	quinone					615:621	the only respiratory quinone	594:621	the only respiratory quinone	594:621	Cells contained summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0 and iso-C17 : 0 3-OH as its major cellular fatty acids and menaquinone-7 as the only respiratory quinone.
31157612	2	51	attach	isolated	128:135	arg1	north					162:166	tundra soil sampled north	142:166	tundra soil sampled north of the Yellow River station, Arctic	142:202	A novel bacterial strain, JDX94T, was isolated from tundra soil sampled north of the Yellow River station, Arctic.
31157612	2	51	attach	isolated	128:135	arg2	JDX94T					116:121	JDX94T	116:121	JDX94T	116:121	A novel bacterial strain, JDX94T, was isolated from tundra soil sampled north of the Yellow River station, Arctic.
31157612	2	51	attach	isolated	128:135	arg2	strain					108:113	A novel bacterial strain	90:113	A novel bacterial strain	90:113	A novel bacterial strain, JDX94T, was isolated from tundra soil sampled north of the Yellow River station, Arctic.
31157612	8	52	theme	JCM	927:929	arg1	15120T					931:936	Pedobacteragri JCM 15120T	912:936	Pedobacteragri JCM 15120T	912:936	On the basis 16S rRNA gene sequence comparison, strain JDX94T showed the highest sequence similarity (96.7 %) to Pedobacteragri JCM 15120T, followed by Pedobacteralluvionis DSM 19624T (96.3 %).
31157612	4	53	dep	optimum	408:414	arg1	pH					417:418	pH 7.0-7.5	417:426	pH 7.0-7.5	417:426	The strain displayed growth at 4-37 °C with an optimum at 28 °C, with 0-1.0 % (w/v) NaCl (optimum, 0%) and at pH 6.0-9.0 (optimum, pH 7.0-7.5).
31157612	5	54	theme	 0	531:532	arg1	3-OH					534:537	 0 3-OH	531:537	iso-C15 : 0 and iso-C17 : 0 3-OH	506:537	Cells contained summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0 and iso-C17 : 0 3-OH as its major cellular fatty acids and menaquinone-7 as the only respiratory quinone.
31157612	2	55	theme	River	182:186	arg1	Arctic					197:202	Arctic	197:202	Arctic	197:202	A novel bacterial strain, JDX94T, was isolated from tundra soil sampled north of the Yellow River station, Arctic.
31157612	2	55	theme	River	182:186	arg1	station					188:194	the Yellow River station	171:194	the Yellow River station	171:194	A novel bacterial strain, JDX94T, was isolated from tundra soil sampled north of the Yellow River station, Arctic.
31157612	2	56	theme	station	188:194	arg1	north					162:166	tundra soil sampled north	142:166	tundra soil sampled north of the Yellow River station, Arctic	142:202	A novel bacterial strain, JDX94T, was isolated from tundra soil sampled north of the Yellow River station, Arctic.
31157612	6	57	theme	unidentified	708:719	arg1	aminolipids					721:731	two unidentified aminolipids	704:731	two unidentified aminolipids	704:731	The polar lipid profile of strain JDX94T consisted of phosphatidylethanolamine, two unidentified aminolipids and four unknown polar lipids.
31157612	7	58	theme	DNA	768:770	arg1	%					796:796	37.5 mol%	788:796	37.5 mol%	788:796	The DNA G+C content was 37.5 mol%.
31157612	7	58	theme	DNA	768:770	arg1	G+C content					772:782	The DNA G+C content	764:782	The DNA G+C content	764:782	The DNA G+C content was 37.5 mol%.
31157612	9	59	theme	%	1160:1160	arg1	%					1176:1176	74.6-79.2 % and 18.9-24.5 %	1150:1176	74.6-79.2 % and 18.9-24.5 %	1150:1176	Furthermore, the average nucleotide identity and digital DNA-DNA hybridization values between strain JDX94T and related species of the genus Pedobacter were 74.6-79.2 % and 18.9-24.5 %, respectively.
31157612	9	59	theme	%	1160:1160	arg1	values					1072:1077	the average nucleotide identity and digital DNA-DNA hybridization values	1006:1077	the average nucleotide identity and digital DNA-DNA hybridization values between strain JDX94T and related species of the genus Pedobacter	1006:1143	Furthermore, the average nucleotide identity and digital DNA-DNA hybridization values between strain JDX94T and related species of the genus Pedobacter were 74.6-79.2 % and 18.9-24.5 %, respectively.
31157612	8	60	theme	Pedobacteralluvionis	951:970	arg1	19624T					976:981	Pedobacteralluvionis DSM 19624T	951:981	Pedobacteralluvionis DSM 19624T (96.3 %)	951:990	On the basis 16S rRNA gene sequence comparison, strain JDX94T showed the highest sequence similarity (96.7 %) to Pedobacteragri JCM 15120T, followed by Pedobacteralluvionis DSM 19624T (96.3 %).
31157612	8	60	theme	Pedobacteralluvionis	951:970	arg1	%					989:989	96.3 %	984:989	96.3 %	984:989	On the basis 16S rRNA gene sequence comparison, strain JDX94T showed the highest sequence similarity (96.7 %) to Pedobacteragri JCM 15120T, followed by Pedobacteralluvionis DSM 19624T (96.3 %).
31157612	2	61	theme	Yellow	175:180	arg1	Arctic					197:202	Arctic	197:202	Arctic	197:202	A novel bacterial strain, JDX94T, was isolated from tundra soil sampled north of the Yellow River station, Arctic.
31157612	2	61	theme	Yellow	175:180	arg1	station					188:194	the Yellow River station	171:194	the Yellow River station	171:194	A novel bacterial strain, JDX94T, was isolated from tundra soil sampled north of the Yellow River station, Arctic.
31157612	8	62	theme	rRNA	816:819	arg1	comparison					835:844	the basis 16S rRNA gene sequence comparison	802:844	the basis 16S rRNA gene sequence comparison	802:844	On the basis 16S rRNA gene sequence comparison, strain JDX94T showed the highest sequence similarity (96.7 %) to Pedobacteragri JCM 15120T, followed by Pedobacteralluvionis DSM 19624T (96.3 %).
31157612	4	63	dep	optimum	376:382	arg1	%					386:386	0%	385:386	0%	385:386	The strain displayed growth at 4-37 °C with an optimum at 28 °C, with 0-1.0 % (w/v) NaCl (optimum, 0%) and at pH 6.0-9.0 (optimum, pH 7.0-7.5).
31157612	9	64	theme	related	1105:1111	arg1	species					1113:1119	related species	1105:1119	related species of the genus Pedobacter	1105:1143	Furthermore, the average nucleotide identity and digital DNA-DNA hybridization values between strain JDX94T and related species of the genus Pedobacter were 74.6-79.2 % and 18.9-24.5 %, respectively.
31157612	5	65	contain	contained	436:444	arg2	quinone					615:621	the only respiratory quinone	594:621	the only respiratory quinone	594:621	Cells contained summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0 and iso-C17 : 0 3-OH as its major cellular fatty acids and menaquinone-7 as the only respiratory quinone.
31157612	5	65	contain	contained	436:444	arg2	acids					567:571	its major cellular fatty acids	542:571	its major cellular fatty acids	542:571	Cells contained summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0 and iso-C17 : 0 3-OH as its major cellular fatty acids and menaquinone-7 as the only respiratory quinone.
31157612	5	65	contain	contained	436:444	arg1	Cells					430:434	Cells	430:434	Cells	430:434	Cells contained summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0 and iso-C17 : 0 3-OH as its major cellular fatty acids and menaquinone-7 as the only respiratory quinone.
31157612	5	65	contain	contained	436:444	arg2	iso-C15 					506:513	iso-C15 	506:513	iso-C15 : 0 and iso-C17 : 0 3-OH	506:537	Cells contained summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0 and iso-C17 : 0 3-OH as its major cellular fatty acids and menaquinone-7 as the only respiratory quinone.
31157612	5	65	contain	contained	436:444	arg2	feature					453:459	summed feature 3	446:461	summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c)	446:503	Cells contained summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0 and iso-C17 : 0 3-OH as its major cellular fatty acids and menaquinone-7 as the only respiratory quinone.
31157612	1	66	from	soil	84:87	arg1	bacterium					55:63	a cellulose-decomposing bacterium	31:63	a cellulose-decomposing bacterium from Arctic tundra soil	31:87	nov., a cellulose-decomposing bacterium from Arctic tundra soil.
31157612	1	66	from	soil	84:87	arg1	nov.					25:28	nov.	25:28	nov.	25:28	nov., a cellulose-decomposing bacterium from Arctic tundra soil.
31157612	11	67	theme	type	1323:1326	arg1	62850T					1364:1369	=MCCC 1H00335T= KCTC 62850T	1343:1369	=MCCC 1H00335T= KCTC 62850T	1343:1369	nov. is suggested, with the type strain JDX94T (=MCCC 1H00335T= KCTC 62850T).
31157612	11	67	theme	type	1323:1326	arg1	JDX94T					1335:1340	the type strain JDX94T	1319:1340	the type strain JDX94T (=MCCC 1H00335T= KCTC 62850T)	1319:1370	nov. is suggested, with the type strain JDX94T (=MCCC 1H00335T= KCTC 62850T).
31157612	11	68	theme	strain	1328:1333	arg1	62850T					1364:1369	=MCCC 1H00335T= KCTC 62850T	1343:1369	=MCCC 1H00335T= KCTC 62850T	1343:1369	nov. is suggested, with the type strain JDX94T (=MCCC 1H00335T= KCTC 62850T).
31157612	11	68	theme	strain	1328:1333	arg1	JDX94T					1335:1340	the type strain JDX94T	1319:1340	the type strain JDX94T (=MCCC 1H00335T= KCTC 62850T)	1319:1370	nov. is suggested, with the type strain JDX94T (=MCCC 1H00335T= KCTC 62850T).
31157612	8	69	theme	DSM	972:974	arg1	19624T					976:981	Pedobacteralluvionis DSM 19624T	951:981	Pedobacteralluvionis DSM 19624T (96.3 %)	951:990	On the basis 16S rRNA gene sequence comparison, strain JDX94T showed the highest sequence similarity (96.7 %) to Pedobacteragri JCM 15120T, followed by Pedobacteralluvionis DSM 19624T (96.3 %).
31157612	8	69	theme	DSM	972:974	arg1	%					989:989	96.3 %	984:989	96.3 %	984:989	On the basis 16S rRNA gene sequence comparison, strain JDX94T showed the highest sequence similarity (96.7 %) to Pedobacteragri JCM 15120T, followed by Pedobacteralluvionis DSM 19624T (96.3 %).
31157612	1	70	theme	cellulose-decomposing	33:53	arg1	bacterium					55:63	a cellulose-decomposing bacterium	31:63	a cellulose-decomposing bacterium from Arctic tundra soil	31:87	nov., a cellulose-decomposing bacterium from Arctic tundra soil.
31157612	1	70	theme	cellulose-decomposing	33:53	arg1	nov.					25:28	nov.	25:28	nov.	25:28	nov., a cellulose-decomposing bacterium from Arctic tundra soil.
31157612	8	71	theme	16S	812:814	arg1	comparison					835:844	the basis 16S rRNA gene sequence comparison	802:844	the basis 16S rRNA gene sequence comparison	802:844	On the basis 16S rRNA gene sequence comparison, strain JDX94T showed the highest sequence similarity (96.7 %) to Pedobacteragri JCM 15120T, followed by Pedobacteralluvionis DSM 19624T (96.3 %).
31157612	5	72	dep	C16 	475:478	arg1	 1ω6c					498:502	 1ω6c	498:502	 1ω6c	498:502	Cells contained summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0 and iso-C17 : 0 3-OH as its major cellular fatty acids and menaquinone-7 as the only respiratory quinone.
31157612	5	72	dep	C16 	475:478	arg1	C16 					493:496	C16 	493:496	C16 	493:496	Cells contained summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0 and iso-C17 : 0 3-OH as its major cellular fatty acids and menaquinone-7 as the only respiratory quinone.
31157612	5	72	dep	C16 	475:478	arg1	 1ω7c					480:484	 1ω7c	480:484	 1ω7c	480:484	Cells contained summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0 and iso-C17 : 0 3-OH as its major cellular fatty acids and menaquinone-7 as the only respiratory quinone.
31157612	11	73	theme	=MCCC	1343:1347	arg1	62850T					1364:1369	=MCCC 1H00335T= KCTC 62850T	1343:1369	=MCCC 1H00335T= KCTC 62850T	1343:1369	nov. is suggested, with the type strain JDX94T (=MCCC 1H00335T= KCTC 62850T).
31157612	11	73	theme	=MCCC	1343:1347	arg1	JDX94T					1335:1340	the type strain JDX94T	1319:1340	the type strain JDX94T (=MCCC 1H00335T= KCTC 62850T)	1319:1370	nov. is suggested, with the type strain JDX94T (=MCCC 1H00335T= KCTC 62850T).
31128871	9	0	theme	concentration	1806:1818	arg1	units					2092:2096	milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units	1773:2096	milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments	1773:2130	Supplementing live yeast to lactating dairy cows did not affect dry matter intake (26.0 kg/d), milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments).
31128871	6	1	theme	milk	1217:1220	arg1	component					1222:1230	milk component	1217:1230	milk component	1217:1230	Dry matter intake, milk yield, and milk component concentrations and yields were analyzed using repeated measures.
31128871	1	2	theme	plausible	230:238	arg1	disturbances					252:263	plausible nutritional disturbances	230:263	plausible nutritional disturbances	230:263	We aimed to evaluate the use of a live-yeast product as a means to attenuate plausible nutritional disturbances when feeding relatively low-forage diets containing rapidly fermentable carbohydrates (i.e., wheat) to high-producing cows in early to mid lactation.
31128871	1	3	dep	mid	400:402	arg1	to					397:398	to	397:398	to	397:398	We aimed to evaluate the use of a live-yeast product as a means to attenuate plausible nutritional disturbances when feeding relatively low-forage diets containing rapidly fermentable carbohydrates (i.e., wheat) to high-producing cows in early to mid lactation.
31128871	9	4	dep	units	2092:2096	arg1	averages					2103:2110	all averages	2099:2110	milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments	1773:2130	Supplementing live yeast to lactating dairy cows did not affect dry matter intake (26.0 kg/d), milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments).
31128871	9	5	theme	lactose	1966:1972	arg1	yield					1974:1978	milk lactose yield	1961:1978	milk lactose yield (2.35 kg/d)	1961:1990	Supplementing live yeast to lactating dairy cows did not affect dry matter intake (26.0 kg/d), milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments).
31128871	9	5	theme	lactose	1966:1972	arg1	kg/d					1986:1989	2.35 kg/d	1981:1989	2.35 kg/d	1981:1989	Supplementing live yeast to lactating dairy cows did not affect dry matter intake (26.0 kg/d), milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments).
31128871	9	6	theme	treatments	2121:2130	arg1	averages					2103:2110	all averages	2099:2110	milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments	1773:2130	Supplementing live yeast to lactating dairy cows did not affect dry matter intake (26.0 kg/d), milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments).
31128871	0	7	theme	Production	0:9	arg1	performance					11:21	Production performance	0:21	Production performance	0:21	Production performance and nutrient digestibility of lactating dairy cows fed low-forage diets with and without the addition of a live-yeast supplement.
31128871	8	8	theme	residual	1662:1669	arg1	error					1671:1675	the random residual error	1651:1675	the random residual error	1651:1675	The statistical model for analyzing BW gain, body condition score gain, and dry matter and nutrient digestibilities included the effects of block, treatment, and the random residual error.
31128871	9	9	theme	milk	1829:1832	arg1	yield					1838:1842	milk fat yield	1829:1842	milk fat yield (1.72 kg/d)	1829:1854	Supplementing live yeast to lactating dairy cows did not affect dry matter intake (26.0 kg/d), milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments).
31128871	9	9	theme	milk	1829:1832	arg1	kg/d					1850:1853	1.72 kg/d	1845:1853	1.72 kg/d	1845:1853	Supplementing live yeast to lactating dairy cows did not affect dry matter intake (26.0 kg/d), milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments).
31128871	10	10	theme	starch	2217:2222	arg1	digestibilities					2138:2152	The digestibilities	2134:2152	The digestibilities of dry matter (70.2%), crude protein (71.4%), NDF (36.4%), and starch (99.8%)	2134:2230	The digestibilities of dry matter (70.2%), crude protein (71.4%), NDF (36.4%), and starch (99.8%) were not affected by treatments.
31128871	11	11	theme	cows	2407:2410	arg1	performance					2344:2354	production performance	2333:2354	production performance	2333:2354	In conclusion, the supplementation of the live yeast did not affect production performance and nutrient digestibility of high-producing dairy cows.
31128871	11	11	theme	cows	2407:2410	arg1	digestibility					2369:2381	nutrient digestibility	2360:2381	nutrient digestibility	2360:2381	In conclusion, the supplementation of the live yeast did not affect production performance and nutrient digestibility of high-producing dairy cows.
31128871	3	12	theme	corn	843:846	arg1	silage					848:853	corn silage	843:853	36.7% corn silage	837:853	The formulated diets contained 36.7% corn silage, 8.3% alfalfa hay, and 55% concentrate.
31128871	2	13	theme	Holstein	631:638	arg1	cows					640:643	Holstein cows	631:643	Holstein cows	631:643	Eight primiparous [mean ± SD; 569 ± 35 kg of body weight (BW) and 80 ± 29 d in milk (DIM) at the beginning of the experiment] and 16 multiparous (665 ± 67 kg of BW and 64 ± 10 DIM at the beginning of the experiment) Holstein cows were blocked by parity and DIM, and randomly assigned to 1 of 2 diets (control vs. yeast) for a 12-wk-long period according to randomized complete block design.
31128871	9	14	theme	yield	1838:1842	arg1	units					2092:2096	milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units	1773:2096	milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments	1773:2130	Supplementing live yeast to lactating dairy cows did not affect dry matter intake (26.0 kg/d), milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments).
31128871	2	15	theme	BW	576:577	arg1	BW					576:577	BW	576:577	BW	576:577	Eight primiparous [mean ± SD; 569 ± 35 kg of body weight (BW) and 80 ± 29 d in milk (DIM) at the beginning of the experiment] and 16 multiparous (665 ± 67 kg of BW and 64 ± 10 DIM at the beginning of the experiment) Holstein cows were blocked by parity and DIM, and randomly assigned to 1 of 2 diets (control vs. yeast) for a 12-wk-long period according to randomized complete block design.
31128871	2	15	theme	BW	576:577	arg1	kg					570:571	kg	570:571	kg	570:571	Eight primiparous [mean ± SD; 569 ± 35 kg of body weight (BW) and 80 ± 29 d in milk (DIM) at the beginning of the experiment] and 16 multiparous (665 ± 67 kg of BW and 64 ± 10 DIM at the beginning of the experiment) Holstein cows were blocked by parity and DIM, and randomly assigned to 1 of 2 diets (control vs. yeast) for a 12-wk-long period according to randomized complete block design.
31128871	12	16	theme	passage	2480:2486	arg1	rate					2488:2491	NDF passage rate	2476:2491	NDF passage rate	2476:2491	A potential interaction between live-yeast supplementation and NDF passage rate, which may have hindered the beneficial effects of live-yeast supplementation on production performance and nutrient utilization, deserves further research.
31128871	6	17	theme	repeated	1278:1285	arg1	measures					1287:1294	repeated measures	1278:1294	repeated measures	1278:1294	Dry matter intake, milk yield, and milk component concentrations and yields were analyzed using repeated measures.
31128871	7	18	theme	treatment	1394:1402	arg1	interaction					1404:1414	treatment interaction	1394:1414	treatment interaction	1394:1414	The statistical model for these variables included the effects of block, treatment, the block by treatment interaction, week, the treatment by week interaction, and the random residual error.
31128871	2	19	from	kg	454:455	arg1	multiparous					548:558	multiparous	548:558	multiparous	548:558	Eight primiparous [mean ± SD; 569 ± 35 kg of body weight (BW) and 80 ± 29 d in milk (DIM) at the beginning of the experiment] and 16 multiparous (665 ± 67 kg of BW and 64 ± 10 DIM at the beginning of the experiment) Holstein cows were blocked by parity and DIM, and randomly assigned to 1 of 2 diets (control vs. yeast) for a 12-wk-long period according to randomized complete block design.
31128871	2	19	from	kg	454:455	arg1	milk					494:497	milk	494:497	milk (DIM) at the beginning of the experiment]	494:539	Eight primiparous [mean ± SD; 569 ± 35 kg of body weight (BW) and 80 ± 29 d in milk (DIM) at the beginning of the experiment] and 16 multiparous (665 ± 67 kg of BW and 64 ± 10 DIM at the beginning of the experiment) Holstein cows were blocked by parity and DIM, and randomly assigned to 1 of 2 diets (control vs. yeast) for a 12-wk-long period according to randomized complete block design.
31128871	2	19	from	kg	454:455	arg1	DIM					500:502	DIM	500:502	DIM	500:502	Eight primiparous [mean ± SD; 569 ± 35 kg of body weight (BW) and 80 ± 29 d in milk (DIM) at the beginning of the experiment] and 16 multiparous (665 ± 67 kg of BW and 64 ± 10 DIM at the beginning of the experiment) Holstein cows were blocked by parity and DIM, and randomly assigned to 1 of 2 diets (control vs. yeast) for a 12-wk-long period according to randomized complete block design.
31128871	2	20	theme	experiment	619:628	arg1	beginning					602:610	the beginning	598:610	the beginning of the experiment	598:628	Eight primiparous [mean ± SD; 569 ± 35 kg of body weight (BW) and 80 ± 29 d in milk (DIM) at the beginning of the experiment] and 16 multiparous (665 ± 67 kg of BW and 64 ± 10 DIM at the beginning of the experiment) Holstein cows were blocked by parity and DIM, and randomly assigned to 1 of 2 diets (control vs. yeast) for a 12-wk-long period according to randomized complete block design.
31128871	6	21	theme	milk	1201:1204	arg1	yield					1206:1210	milk yield	1201:1210	milk yield	1201:1210	Dry matter intake, milk yield, and milk component concentrations and yields were analyzed using repeated measures.
31128871	1	22	theme	product	198:204	arg1	means					211:215	a means	209:215	a means to attenuate plausible nutritional disturbances when feeding relatively low-forage diets containing rapidly fermentable carbohydrates (i.e., wheat) to high-producing cows in early to mid lactation	209:412	We aimed to evaluate the use of a live-yeast product as a means to attenuate plausible nutritional disturbances when feeding relatively low-forage diets containing rapidly fermentable carbohydrates (i.e., wheat) to high-producing cows in early to mid lactation.
31128871	1	22	theme	product	198:204	arg1	use					178:180	the use	174:180	the use of a live-yeast product	174:204	We aimed to evaluate the use of a live-yeast product as a means to attenuate plausible nutritional disturbances when feeding relatively low-forage diets containing rapidly fermentable carbohydrates (i.e., wheat) to high-producing cows in early to mid lactation.
31128871	7	23	theme	block	1385:1389	arg1	effects					1352:1358	the effects	1348:1358	the effects of block, treatment, the block by treatment interaction, week, the treatment by week interaction, and the random residual error	1348:1486	The statistical model for these variables included the effects of block, treatment, the block by treatment interaction, week, the treatment by week interaction, and the random residual error.
31128871	4	24	theme	1011	956:959	arg1	cfu/d					961:965	approximately 5.4 × 1011 cfu/d	936:965	approximately 5.4 × 1011 cfu/d of Saccharomyces cerevisiae (BeneSacc; Global Nutritech Biotechnology LLC, Richmond, VA)	936:1054	The yeast diet was formulated to provide approximately 5.4 × 1011 cfu/d of Saccharomyces cerevisiae (BeneSacc; Global Nutritech Biotechnology LLC, Richmond, VA).
31128871	0	25	theme	supplement	141:150	arg1	addition					116:123	the addition	112:123	the addition of a live-yeast supplement	112:150	Production performance and nutrient digestibility of lactating dairy cows fed low-forage diets with and without the addition of a live-yeast supplement.
31128871	12	26	theme	live-yeast	2445:2454	arg1	supplementation					2456:2470	live-yeast supplementation	2445:2470	live-yeast supplementation	2445:2470	A potential interaction between live-yeast supplementation and NDF passage rate, which may have hindered the beneficial effects of live-yeast supplementation on production performance and nutrient utilization, deserves further research.
31128871	8	27	theme	BW	1525:1526	arg1	gain					1528:1531	BW gain	1525:1531	BW gain	1525:1531	The statistical model for analyzing BW gain, body condition score gain, and dry matter and nutrient digestibilities included the effects of block, treatment, and the random residual error.
31128871	9	28	theme	lactating	1706:1714	arg1	cows					1722:1725	lactating dairy cows	1706:1725	lactating dairy cows	1706:1725	Supplementing live yeast to lactating dairy cows did not affect dry matter intake (26.0 kg/d), milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments).
31128871	9	29	theme	milk	1857:1860	arg1	concentration					1870:1882	milk protein concentration	1857:1882	milk protein concentration (2.96%)	1857:1890	Supplementing live yeast to lactating dairy cows did not affect dry matter intake (26.0 kg/d), milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments).
31128871	9	29	theme	milk	1857:1860	arg1	%					1889:1889	2.96%	1885:1889	2.96%	1885:1889	Supplementing live yeast to lactating dairy cows did not affect dry matter intake (26.0 kg/d), milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments).
31128871	7	30	theme	error	1482:1486	arg1	effects					1352:1358	the effects	1348:1358	the effects of block, treatment, the block by treatment interaction, week, the treatment by week interaction, and the random residual error	1348:1486	The statistical model for these variables included the effects of block, treatment, the block by treatment interaction, week, the treatment by week interaction, and the random residual error.
31128871	2	31	theme	weight	465:470	arg1	BW					473:474	BW	473:474	BW	473:474	Eight primiparous [mean ± SD; 569 ± 35 kg of body weight (BW) and 80 ± 29 d in milk (DIM) at the beginning of the experiment] and 16 multiparous (665 ± 67 kg of BW and 64 ± 10 DIM at the beginning of the experiment) Holstein cows were blocked by parity and DIM, and randomly assigned to 1 of 2 diets (control vs. yeast) for a 12-wk-long period according to randomized complete block design.
31128871	2	31	theme	weight	465:470	arg1	kg					454:455	kg	454:455	kg	454:455	Eight primiparous [mean ± SD; 569 ± 35 kg of body weight (BW) and 80 ± 29 d in milk (DIM) at the beginning of the experiment] and 16 multiparous (665 ± 67 kg of BW and 64 ± 10 DIM at the beginning of the experiment) Holstein cows were blocked by parity and DIM, and randomly assigned to 1 of 2 diets (control vs. yeast) for a 12-wk-long period according to randomized complete block design.
31128871	2	31	theme	weight	465:470	arg1	weight					465:470	body weight	460:470	body weight (BW)	460:475	Eight primiparous [mean ± SD; 569 ± 35 kg of body weight (BW) and 80 ± 29 d in milk (DIM) at the beginning of the experiment] and 16 multiparous (665 ± 67 kg of BW and 64 ± 10 DIM at the beginning of the experiment) Holstein cows were blocked by parity and DIM, and randomly assigned to 1 of 2 diets (control vs. yeast) for a 12-wk-long period according to randomized complete block design.
31128871	10	32	theme	protein	2183:2189	arg1	digestibilities					2138:2152	The digestibilities	2134:2152	The digestibilities of dry matter (70.2%), crude protein (71.4%), NDF (36.4%), and starch (99.8%)	2134:2230	The digestibilities of dry matter (70.2%), crude protein (71.4%), NDF (36.4%), and starch (99.8%) were not affected by treatments.
31128871	5	33	theme	nutrient	1069:1076	arg1	digestibility					1078:1090	Total-tract nutrient digestibility	1057:1090	Total-tract nutrient digestibility	1057:1090	Total-tract nutrient digestibility was estimated using 240-h undigested neutral detergent fiber (NDF) as an internal marker.
31128871	9	34	theme	concentration	1870:1882	arg1	units					2092:2096	milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units	1773:2096	milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments	1773:2130	Supplementing live yeast to lactating dairy cows did not affect dry matter intake (26.0 kg/d), milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments).
31128871	0	35	theme	cows	69:72	arg1	performance					11:21	Production performance	0:21	Production performance	0:21	Production performance and nutrient digestibility of lactating dairy cows fed low-forage diets with and without the addition of a live-yeast supplement.
31128871	0	35	theme	cows	69:72	arg1	digestibility					36:48	nutrient digestibility	27:48	nutrient digestibility	27:48	Production performance and nutrient digestibility of lactating dairy cows fed low-forage diets with and without the addition of a live-yeast supplement.
31128871	8	36	theme	condition	1539:1547	arg1	gain					1555:1558	body condition score gain	1534:1558	body condition score gain	1534:1558	The statistical model for analyzing BW gain, body condition score gain, and dry matter and nutrient digestibilities included the effects of block, treatment, and the random residual error.
31128871	7	37	theme	random	1466:1471	arg1	error					1482:1486	the random residual error	1462:1486	the random residual error	1462:1486	The statistical model for these variables included the effects of block, treatment, the block by treatment interaction, week, the treatment by week interaction, and the random residual error.
31128871	2	38	theme	randomized	772:781	arg1	design					798:803	randomized complete block design	772:803	randomized complete block design	772:803	Eight primiparous [mean ± SD; 569 ± 35 kg of body weight (BW) and 80 ± 29 d in milk (DIM) at the beginning of the experiment] and 16 multiparous (665 ± 67 kg of BW and 64 ± 10 DIM at the beginning of the experiment) Holstein cows were blocked by parity and DIM, and randomly assigned to 1 of 2 diets (control vs. yeast) for a 12-wk-long period according to randomized complete block design.
31128871	0	39	theme	low-forage	78:87	arg1	diets					89:93	low-forage diets	78:93	low-forage diets with and without the addition of a live-yeast supplement	78:150	Production performance and nutrient digestibility of lactating dairy cows fed low-forage diets with and without the addition of a live-yeast supplement.
31128871	9	40	theme	weight	2031:2036	arg1	gain					2038:2041	body weight gain	2026:2041	body weight gain (0.62 kg/d)	2026:2053	Supplementing live yeast to lactating dairy cows did not affect dry matter intake (26.0 kg/d), milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments).
31128871	9	40	theme	weight	2031:2036	arg1	kg/d					2049:2052	0.62 kg/d	2044:2052	0.62 kg/d	2044:2052	Supplementing live yeast to lactating dairy cows did not affect dry matter intake (26.0 kg/d), milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments).
31128871	4	41	theme	Global	1006:1011	arg1	LLC					1037:1039	Global Nutritech Biotechnology LLC	1006:1039	Global Nutritech Biotechnology LLC	1006:1039	The yeast diet was formulated to provide approximately 5.4 × 1011 cfu/d of Saccharomyces cerevisiae (BeneSacc; Global Nutritech Biotechnology LLC, Richmond, VA).
31128871	4	42	theme	Biotechnology	1023:1035	arg1	LLC					1037:1039	Global Nutritech Biotechnology LLC	1006:1039	Global Nutritech Biotechnology LLC	1006:1039	The yeast diet was formulated to provide approximately 5.4 × 1011 cfu/d of Saccharomyces cerevisiae (BeneSacc; Global Nutritech Biotechnology LLC, Richmond, VA).
31128871	5	43	theme	undigested	1118:1127	arg1	NDF					1154:1156	NDF	1154:1156	NDF	1154:1156	Total-tract nutrient digestibility was estimated using 240-h undigested neutral detergent fiber (NDF) as an internal marker.
31128871	5	43	theme	undigested	1118:1127	arg1	fiber					1147:1151	240-h undigested neutral detergent fiber	1112:1151	240-h undigested neutral detergent fiber (NDF)	1112:1157	Total-tract nutrient digestibility was estimated using 240-h undigested neutral detergent fiber (NDF) as an internal marker.
31128871	5	43	theme	undigested	1118:1127	arg1	marker					1174:1179	an internal marker	1162:1179	an internal marker	1162:1179	Total-tract nutrient digestibility was estimated using 240-h undigested neutral detergent fiber (NDF) as an internal marker.
31128871	9	44	theme	milk	1893:1896	arg1	yield					1906:1910	milk protein yield	1893:1910	milk protein yield (1.43 kg/d)	1893:1922	Supplementing live yeast to lactating dairy cows did not affect dry matter intake (26.0 kg/d), milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments).
31128871	9	44	theme	milk	1893:1896	arg1	kg/d					1918:1921	1.43 kg/d	1913:1921	1.43 kg/d	1913:1921	Supplementing live yeast to lactating dairy cows did not affect dry matter intake (26.0 kg/d), milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments).
31128871	11	45	theme	dairy	2401:2405	arg1	cows					2407:2410	high-producing dairy cows	2386:2410	high-producing dairy cows	2386:2410	In conclusion, the supplementation of the live yeast did not affect production performance and nutrient digestibility of high-producing dairy cows.
31128871	2	46	theme	block	792:796	arg1	design					798:803	randomized complete block design	772:803	randomized complete block design	772:803	Eight primiparous [mean ± SD; 569 ± 35 kg of body weight (BW) and 80 ± 29 d in milk (DIM) at the beginning of the experiment] and 16 multiparous (665 ± 67 kg of BW and 64 ± 10 DIM at the beginning of the experiment) Holstein cows were blocked by parity and DIM, and randomly assigned to 1 of 2 diets (control vs. yeast) for a 12-wk-long period according to randomized complete block design.
31128871	4	47	theme	cerevisiae	984:993	arg1	cfu/d					961:965	approximately 5.4 × 1011 cfu/d	936:965	approximately 5.4 × 1011 cfu/d of Saccharomyces cerevisiae (BeneSacc; Global Nutritech Biotechnology LLC, Richmond, VA)	936:1054	The yeast diet was formulated to provide approximately 5.4 × 1011 cfu/d of Saccharomyces cerevisiae (BeneSacc; Global Nutritech Biotechnology LLC, Richmond, VA).
31128871	5	48	theme	detergent	1137:1145	arg1	NDF					1154:1156	NDF	1154:1156	NDF	1154:1156	Total-tract nutrient digestibility was estimated using 240-h undigested neutral detergent fiber (NDF) as an internal marker.
31128871	5	48	theme	detergent	1137:1145	arg1	fiber					1147:1151	240-h undigested neutral detergent fiber	1112:1151	240-h undigested neutral detergent fiber (NDF)	1112:1157	Total-tract nutrient digestibility was estimated using 240-h undigested neutral detergent fiber (NDF) as an internal marker.
31128871	5	48	theme	detergent	1137:1145	arg1	marker					1174:1179	an internal marker	1162:1179	an internal marker	1162:1179	Total-tract nutrient digestibility was estimated using 240-h undigested neutral detergent fiber (NDF) as an internal marker.
31128871	9	49	theme	yield	1906:1910	arg1	units					2092:2096	milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units	1773:2096	milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments	1773:2130	Supplementing live yeast to lactating dairy cows did not affect dry matter intake (26.0 kg/d), milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments).
31128871	3	50	dep	%	859:859	arg1	hay					869:871	hay	869:871	8.3% alfalfa hay	856:871	The formulated diets contained 36.7% corn silage, 8.3% alfalfa hay, and 55% concentrate.
31128871	2	51	dep	±	439:439	arg1	SD					441:442	SD	441:442	Eight primiparous [mean ± SD; 569 ± 35 kg of body weight (BW) and 80 ± 29 d in milk (DIM) at the beginning of the experiment] and 16 multiparous (665 ± 67 kg of BW and 64 ± 10 DIM at the beginning of the experiment) Holstein cows	415:643	Eight primiparous [mean ± SD; 569 ± 35 kg of body weight (BW) and 80 ± 29 d in milk (DIM) at the beginning of the experiment] and 16 multiparous (665 ± 67 kg of BW and 64 ± 10 DIM at the beginning of the experiment) Holstein cows were blocked by parity and DIM, and randomly assigned to 1 of 2 diets (control vs. yeast) for a 12-wk-long period according to randomized complete block design.
31128871	8	52	theme	nutrient	1580:1587	arg1	digestibilities					1589:1603	nutrient digestibilities	1580:1603	nutrient digestibilities	1580:1603	The statistical model for analyzing BW gain, body condition score gain, and dry matter and nutrient digestibilities included the effects of block, treatment, and the random residual error.
31128871	9	53	theme	milk	1773:1776	arg1	yield					1778:1782	milk yield	1773:1782	milk yield (48.1 kg/d)	1773:1794	Supplementing live yeast to lactating dairy cows did not affect dry matter intake (26.0 kg/d), milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments).
31128871	9	53	theme	milk	1773:1776	arg1	kg/d					1790:1793	48.1 kg/d	1785:1793	48.1 kg/d	1785:1793	Supplementing live yeast to lactating dairy cows did not affect dry matter intake (26.0 kg/d), milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments).
31128871	2	54	theme	[mean	433:437	arg1	±					439:439	Eight primiparous [mean ±	415:439	Eight primiparous [mean ± SD; 569 ± 35 kg of body weight (BW) and 80 ± 29 d in milk (DIM) at the beginning of the experiment] and 16 multiparous (665 ± 67 kg of BW and 64 ± 10 DIM at the beginning of the experiment) Holstein cows	415:643	Eight primiparous [mean ± SD; 569 ± 35 kg of body weight (BW) and 80 ± 29 d in milk (DIM) at the beginning of the experiment] and 16 multiparous (665 ± 67 kg of BW and 64 ± 10 DIM at the beginning of the experiment) Holstein cows were blocked by parity and DIM, and randomly assigned to 1 of 2 diets (control vs. yeast) for a 12-wk-long period according to randomized complete block design.
31128871	9	55	theme	body	2060:2063	arg1	gain					2081:2084	body condition score gain	2060:2084	body condition score gain	2060:2084	Supplementing live yeast to lactating dairy cows did not affect dry matter intake (26.0 kg/d), milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments).
31128871	7	56	theme	week	1417:1420	arg1	effects					1352:1358	the effects	1348:1358	the effects of block, treatment, the block by treatment interaction, week, the treatment by week interaction, and the random residual error	1348:1486	The statistical model for these variables included the effects of block, treatment, the block by treatment interaction, week, the treatment by week interaction, and the random residual error.
31128871	2	57	dep	kg	570:571	arg1	DIM					591:593	10 DIM	588:593	10 DIM	588:593	Eight primiparous [mean ± SD; 569 ± 35 kg of body weight (BW) and 80 ± 29 d in milk (DIM) at the beginning of the experiment] and 16 multiparous (665 ± 67 kg of BW and 64 ± 10 DIM at the beginning of the experiment) Holstein cows were blocked by parity and DIM, and randomly assigned to 1 of 2 diets (control vs. yeast) for a 12-wk-long period according to randomized complete block design.
31128871	2	58	from	beginning	602:610	arg1	BW					576:577	BW	576:577	BW	576:577	Eight primiparous [mean ± SD; 569 ± 35 kg of body weight (BW) and 80 ± 29 d in milk (DIM) at the beginning of the experiment] and 16 multiparous (665 ± 67 kg of BW and 64 ± 10 DIM at the beginning of the experiment) Holstein cows were blocked by parity and DIM, and randomly assigned to 1 of 2 diets (control vs. yeast) for a 12-wk-long period according to randomized complete block design.
31128871	2	58	from	beginning	602:610	arg1	kg					570:571	kg	570:571	kg	570:571	Eight primiparous [mean ± SD; 569 ± 35 kg of body weight (BW) and 80 ± 29 d in milk (DIM) at the beginning of the experiment] and 16 multiparous (665 ± 67 kg of BW and 64 ± 10 DIM at the beginning of the experiment) Holstein cows were blocked by parity and DIM, and randomly assigned to 1 of 2 diets (control vs. yeast) for a 12-wk-long period according to randomized complete block design.
31128871	6	59	theme	matter	1186:1191	arg1	intake					1193:1198	matter intake	1186:1198	matter intake	1186:1198	Dry matter intake, milk yield, and milk component concentrations and yields were analyzed using repeated measures.
31128871	9	60	theme	score	2075:2079	arg1	gain					2081:2084	body condition score gain	2060:2084	body condition score gain	2060:2084	Supplementing live yeast to lactating dairy cows did not affect dry matter intake (26.0 kg/d), milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments).
31128871	2	61	theme	12-wk-long	741:750	arg1	period					752:757	a 12-wk-long period	739:757	a 12-wk-long period	739:757	Eight primiparous [mean ± SD; 569 ± 35 kg of body weight (BW) and 80 ± 29 d in milk (DIM) at the beginning of the experiment] and 16 multiparous (665 ± 67 kg of BW and 64 ± 10 DIM at the beginning of the experiment) Holstein cows were blocked by parity and DIM, and randomly assigned to 1 of 2 diets (control vs. yeast) for a 12-wk-long period according to randomized complete block design.
31128871	2	62	dep	diets	709:713	arg1	yeast					728:732	yeast	728:732	yeast	728:732	Eight primiparous [mean ± SD; 569 ± 35 kg of body weight (BW) and 80 ± 29 d in milk (DIM) at the beginning of the experiment] and 16 multiparous (665 ± 67 kg of BW and 64 ± 10 DIM at the beginning of the experiment) Holstein cows were blocked by parity and DIM, and randomly assigned to 1 of 2 diets (control vs. yeast) for a 12-wk-long period according to randomized complete block design.
31128871	2	62	dep	diets	709:713	arg1	control					716:722	control	716:722	control	716:722	Eight primiparous [mean ± SD; 569 ± 35 kg of body weight (BW) and 80 ± 29 d in milk (DIM) at the beginning of the experiment] and 16 multiparous (665 ± 67 kg of BW and 64 ± 10 DIM at the beginning of the experiment) Holstein cows were blocked by parity and DIM, and randomly assigned to 1 of 2 diets (control vs. yeast) for a 12-wk-long period according to randomized complete block design.
31128871	9	63	theme	milk	1925:1928	arg1	concentration					1938:1950	milk lactose concentration	1925:1950	milk lactose concentration (4.84%)	1925:1958	Supplementing live yeast to lactating dairy cows did not affect dry matter intake (26.0 kg/d), milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments).
31128871	9	63	theme	milk	1925:1928	arg1	%					1957:1957	4.84%	1953:1957	4.84%	1953:1957	Supplementing live yeast to lactating dairy cows did not affect dry matter intake (26.0 kg/d), milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments).
31128871	0	64	theme	lactating	53:61	arg1	cows					69:72	lactating dairy cows	53:72	lactating dairy cows	53:72	Production performance and nutrient digestibility of lactating dairy cows fed low-forage diets with and without the addition of a live-yeast supplement.
31128871	2	65	dep	kg	454:455	arg1	d					489:489	29 d	486:489	29 d	486:489	Eight primiparous [mean ± SD; 569 ± 35 kg of body weight (BW) and 80 ± 29 d in milk (DIM) at the beginning of the experiment] and 16 multiparous (665 ± 67 kg of BW and 64 ± 10 DIM at the beginning of the experiment) Holstein cows were blocked by parity and DIM, and randomly assigned to 1 of 2 diets (control vs. yeast) for a 12-wk-long period according to randomized complete block design.
31128871	12	66	theme	further	2632:2638	arg1	research					2640:2647	further research	2632:2647	further research	2632:2647	A potential interaction between live-yeast supplementation and NDF passage rate, which may have hindered the beneficial effects of live-yeast supplementation on production performance and nutrient utilization, deserves further research.
31128871	12	67	theme	production	2574:2583	arg1	performance					2585:2595	production performance	2574:2595	production performance	2574:2595	A potential interaction between live-yeast supplementation and NDF passage rate, which may have hindered the beneficial effects of live-yeast supplementation on production performance and nutrient utilization, deserves further research.
31128871	9	68	theme	concentration	1938:1950	arg1	units					2092:2096	milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units	1773:2096	milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments	1773:2130	Supplementing live yeast to lactating dairy cows did not affect dry matter intake (26.0 kg/d), milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments).
31128871	4	69	dep	BeneSacc	996:1003	arg1	LLC					1037:1039	Global Nutritech Biotechnology LLC	1006:1039	Global Nutritech Biotechnology LLC	1006:1039	The yeast diet was formulated to provide approximately 5.4 × 1011 cfu/d of Saccharomyces cerevisiae (BeneSacc; Global Nutritech Biotechnology LLC, Richmond, VA).
31128871	10	70	theme	matter	2161:2166	arg1	digestibilities					2138:2152	The digestibilities	2134:2152	The digestibilities of dry matter (70.2%), crude protein (71.4%), NDF (36.4%), and starch (99.8%)	2134:2230	The digestibilities of dry matter (70.2%), crude protein (71.4%), NDF (36.4%), and starch (99.8%) were not affected by treatments.
31128871	4	71	dep	cerevisiae	984:993	arg1	BeneSacc					996:1003	BeneSacc	996:1003	BeneSacc	996:1003	The yeast diet was formulated to provide approximately 5.4 × 1011 cfu/d of Saccharomyces cerevisiae (BeneSacc; Global Nutritech Biotechnology LLC, Richmond, VA).
31128871	4	71	dep	cerevisiae	984:993	arg1	VA					1052:1053	VA	1052:1053	VA	1052:1053	The yeast diet was formulated to provide approximately 5.4 × 1011 cfu/d of Saccharomyces cerevisiae (BeneSacc; Global Nutritech Biotechnology LLC, Richmond, VA).
31128871	0	72	theme	dairy	63:67	arg1	cows					69:72	lactating dairy cows	53:72	lactating dairy cows	53:72	Production performance and nutrient digestibility of lactating dairy cows fed low-forage diets with and without the addition of a live-yeast supplement.
31128871	9	73	theme	fat	1802:1804	arg1	concentration					1806:1818	milk fat concentration	1797:1818	milk fat concentration (3.61%)	1797:1826	Supplementing live yeast to lactating dairy cows did not affect dry matter intake (26.0 kg/d), milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments).
31128871	9	73	theme	fat	1802:1804	arg1	%					1825:1825	3.61%	1821:1825	3.61%	1821:1825	Supplementing live yeast to lactating dairy cows did not affect dry matter intake (26.0 kg/d), milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments).
31128871	6	74	theme	component	1222:1230	arg1	concentrations					1232:1245	Dry matter intake, milk yield, and milk component concentrations	1182:1245	Dry matter intake, milk yield, and milk component concentrations	1182:1245	Dry matter intake, milk yield, and milk component concentrations and yields were analyzed using repeated measures.
31128871	10	75	theme	NDF	2200:2202	arg1	digestibilities					2138:2152	The digestibilities	2134:2152	The digestibilities of dry matter (70.2%), crude protein (71.4%), NDF (36.4%), and starch (99.8%)	2134:2230	The digestibilities of dry matter (70.2%), crude protein (71.4%), NDF (36.4%), and starch (99.8%) were not affected by treatments.
31128871	12	76	from	effects	2533:2539	arg1	performance					2585:2595	production performance	2574:2595	production performance	2574:2595	A potential interaction between live-yeast supplementation and NDF passage rate, which may have hindered the beneficial effects of live-yeast supplementation on production performance and nutrient utilization, deserves further research.
31128871	12	76	from	effects	2533:2539	arg1	utilization					2610:2620	nutrient utilization	2601:2620	nutrient utilization	2601:2620	A potential interaction between live-yeast supplementation and NDF passage rate, which may have hindered the beneficial effects of live-yeast supplementation on production performance and nutrient utilization, deserves further research.
31128871	12	77	theme	supplementation	2555:2569	arg1	effects					2533:2539	the beneficial effects	2518:2539	the beneficial effects of live-yeast supplementation on production performance and nutrient utilization	2518:2620	A potential interaction between live-yeast supplementation and NDF passage rate, which may have hindered the beneficial effects of live-yeast supplementation on production performance and nutrient utilization, deserves further research.
31128871	9	78	theme	milk	1961:1964	arg1	yield					1974:1978	milk lactose yield	1961:1978	milk lactose yield (2.35 kg/d)	1961:1990	Supplementing live yeast to lactating dairy cows did not affect dry matter intake (26.0 kg/d), milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments).
31128871	9	78	theme	milk	1961:1964	arg1	kg/d					1986:1989	2.35 kg/d	1981:1989	2.35 kg/d	1981:1989	Supplementing live yeast to lactating dairy cows did not affect dry matter intake (26.0 kg/d), milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments).
31128871	1	79	theme	nutritional	240:250	arg1	disturbances					252:263	plausible nutritional disturbances	230:263	plausible nutritional disturbances	230:263	We aimed to evaluate the use of a live-yeast product as a means to attenuate plausible nutritional disturbances when feeding relatively low-forage diets containing rapidly fermentable carbohydrates (i.e., wheat) to high-producing cows in early to mid lactation.
31128871	3	80	theme	formulated	810:819	arg1	diets					821:825	The formulated diets	806:825	The formulated diets	806:825	The formulated diets contained 36.7% corn silage, 8.3% alfalfa hay, and 55% concentrate.
31128871	8	81	theme	block	1629:1633	arg1	effects					1618:1624	the effects	1614:1624	the effects of block, treatment, and the random residual error	1614:1675	The statistical model for analyzing BW gain, body condition score gain, and dry matter and nutrient digestibilities included the effects of block, treatment, and the random residual error.
31128871	9	82	theme	yield	1974:1978	arg1	units					2092:2096	milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units	1773:2096	milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments	1773:2130	Supplementing live yeast to lactating dairy cows did not affect dry matter intake (26.0 kg/d), milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments).
31128871	3	83	dep	%	880:880	arg1	concentrate					882:892	concentrate	882:892	55% concentrate	878:892	The formulated diets contained 36.7% corn silage, 8.3% alfalfa hay, and 55% concentrate.
31128871	2	84	theme	experiment	529:538	arg1	beginning					512:520	the beginning	508:520	the beginning of the experiment	508:538	Eight primiparous [mean ± SD; 569 ± 35 kg of body weight (BW) and 80 ± 29 d in milk (DIM) at the beginning of the experiment] and 16 multiparous (665 ± 67 kg of BW and 64 ± 10 DIM at the beginning of the experiment) Holstein cows were blocked by parity and DIM, and randomly assigned to 1 of 2 diets (control vs. yeast) for a 12-wk-long period according to randomized complete block design.
31128871	11	85	theme	live	2307:2310	arg1	yeast					2312:2316	the live yeast	2303:2316	the live yeast	2303:2316	In conclusion, the supplementation of the live yeast did not affect production performance and nutrient digestibility of high-producing dairy cows.
31128871	12	86	theme	beneficial	2522:2531	arg1	effects					2533:2539	the beneficial effects	2518:2539	the beneficial effects of live-yeast supplementation on production performance and nutrient utilization	2518:2620	A potential interaction between live-yeast supplementation and NDF passage rate, which may have hindered the beneficial effects of live-yeast supplementation on production performance and nutrient utilization, deserves further research.
31128871	8	87	theme	treatment	1636:1644	arg1	effects					1618:1624	the effects	1614:1624	the effects of block, treatment, and the random residual error	1614:1675	The statistical model for analyzing BW gain, body condition score gain, and dry matter and nutrient digestibilities included the effects of block, treatment, and the random residual error.
31128871	12	88	theme	NDF	2476:2478	arg1	rate					2488:2491	NDF passage rate	2476:2491	NDF passage rate	2476:2491	A potential interaction between live-yeast supplementation and NDF passage rate, which may have hindered the beneficial effects of live-yeast supplementation on production performance and nutrient utilization, deserves further research.
31128871	11	89	theme	nutrient	2360:2367	arg1	digestibility					2369:2381	nutrient digestibility	2360:2381	nutrient digestibility	2360:2381	In conclusion, the supplementation of the live yeast did not affect production performance and nutrient digestibility of high-producing dairy cows.
31128871	9	90	theme	fat	1834:1836	arg1	yield					1838:1842	milk fat yield	1829:1842	milk fat yield (1.72 kg/d)	1829:1854	Supplementing live yeast to lactating dairy cows did not affect dry matter intake (26.0 kg/d), milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments).
31128871	9	90	theme	fat	1834:1836	arg1	kg/d					1850:1853	1.72 kg/d	1845:1853	1.72 kg/d	1845:1853	Supplementing live yeast to lactating dairy cows did not affect dry matter intake (26.0 kg/d), milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments).
31128871	1	91	contain	containing	306:315	arg2	carbohydrates					337:349	rapidly fermentable carbohydrates	317:349	rapidly fermentable carbohydrates (i.e., wheat) to high-producing cows	317:386	We aimed to evaluate the use of a live-yeast product as a means to attenuate plausible nutritional disturbances when feeding relatively low-forage diets containing rapidly fermentable carbohydrates (i.e., wheat) to high-producing cows in early to mid lactation.
31128871	1	91	contain	containing	306:315	arg1	diets					300:304	relatively low-forage diets	278:304	relatively low-forage diets containing rapidly fermentable carbohydrates (i.e., wheat) to high-producing cows in early to mid lactation	278:412	We aimed to evaluate the use of a live-yeast product as a means to attenuate plausible nutritional disturbances when feeding relatively low-forage diets containing rapidly fermentable carbohydrates (i.e., wheat) to high-producing cows in early to mid lactation.
31128871	10	92	theme	crude	2177:2181	arg1	protein					2183:2189	crude protein	2177:2189	crude protein (71.4%)	2177:2197	The digestibilities of dry matter (70.2%), crude protein (71.4%), NDF (36.4%), and starch (99.8%) were not affected by treatments.
31128871	10	92	theme	crude	2177:2181	arg1	%					2196:2196	71.4%	2192:2196	71.4%	2192:2196	The digestibilities of dry matter (70.2%), crude protein (71.4%), NDF (36.4%), and starch (99.8%) were not affected by treatments.
31128871	4	93	theme	yeast	899:903	arg1	diet					905:908	The yeast diet	895:908	The yeast diet	895:908	The yeast diet was formulated to provide approximately 5.4 × 1011 cfu/d of Saccharomyces cerevisiae (BeneSacc; Global Nutritech Biotechnology LLC, Richmond, VA).
31128871	0	94	theme	live-yeast	130:139	arg1	supplement					141:150	a live-yeast supplement	128:150	a live-yeast supplement	128:150	Production performance and nutrient digestibility of lactating dairy cows fed low-forage diets with and without the addition of a live-yeast supplement.
31128871	9	95	theme	milk	1993:1996	arg1	nitrogen					2003:2010	milk urea nitrogen	1993:2010	milk urea nitrogen (7.99 mg/dL)	1993:2023	Supplementing live yeast to lactating dairy cows did not affect dry matter intake (26.0 kg/d), milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments).
31128871	9	95	theme	milk	1993:1996	arg1	mg/dL					2018:2022	7.99 mg/dL	2013:2022	7.99 mg/dL	2013:2022	Supplementing live yeast to lactating dairy cows did not affect dry matter intake (26.0 kg/d), milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments).
31128871	1	96	theme	live-yeast	187:196	arg1	product					198:204	a live-yeast product	185:204	a live-yeast product	185:204	We aimed to evaluate the use of a live-yeast product as a means to attenuate plausible nutritional disturbances when feeding relatively low-forage diets containing rapidly fermentable carbohydrates (i.e., wheat) to high-producing cows in early to mid lactation.
31128871	9	97	theme	live	1692:1695	arg1	yeast					1697:1701	live yeast	1692:1701	live yeast to lactating dairy cows	1692:1725	Supplementing live yeast to lactating dairy cows did not affect dry matter intake (26.0 kg/d), milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments).
31128871	8	98	theme	error	1671:1675	arg1	effects					1618:1624	the effects	1614:1624	the effects of block, treatment, and the random residual error	1614:1675	The statistical model for analyzing BW gain, body condition score gain, and dry matter and nutrient digestibilities included the effects of block, treatment, and the random residual error.
31128871	9	99	theme	nitrogen	2003:2010	arg1	units					2092:2096	milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units	1773:2096	milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments	1773:2130	Supplementing live yeast to lactating dairy cows did not affect dry matter intake (26.0 kg/d), milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments).
31128871	7	100	theme	treatment	1370:1378	arg1	effects					1352:1358	the effects	1348:1358	the effects of block, treatment, the block by treatment interaction, week, the treatment by week interaction, and the random residual error	1348:1486	The statistical model for these variables included the effects of block, treatment, the block by treatment interaction, week, the treatment by week interaction, and the random residual error.
31128871	4	101	theme	Saccharomyces	970:982	arg1	cerevisiae					984:993	Saccharomyces cerevisiae	970:993	Saccharomyces cerevisiae (BeneSacc; Global Nutritech Biotechnology LLC, Richmond, VA)	970:1054	The yeast diet was formulated to provide approximately 5.4 × 1011 cfu/d of Saccharomyces cerevisiae (BeneSacc; Global Nutritech Biotechnology LLC, Richmond, VA).
31128871	11	102	theme	production	2333:2342	arg1	performance					2344:2354	production performance	2333:2354	production performance	2333:2354	In conclusion, the supplementation of the live yeast did not affect production performance and nutrient digestibility of high-producing dairy cows.
31128871	9	103	theme	dry	1742:1744	arg1	intake					1753:1758	dry matter intake	1742:1758	dry matter intake (26.0 kg/d), milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments)	1742:2131	Supplementing live yeast to lactating dairy cows did not affect dry matter intake (26.0 kg/d), milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments).
31128871	9	103	theme	dry	1742:1744	arg1	kg/d					1766:1769	26.0 kg/d	1761:1769	26.0 kg/d	1761:1769	Supplementing live yeast to lactating dairy cows did not affect dry matter intake (26.0 kg/d), milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments).
31128871	7	104	theme	block	1363:1367	arg1	effects					1352:1358	the effects	1348:1358	the effects of block, treatment, the block by treatment interaction, week, the treatment by week interaction, and the random residual error	1348:1486	The statistical model for these variables included the effects of block, treatment, the block by treatment interaction, week, the treatment by week interaction, and the random residual error.
31128871	9	105	theme	dairy	1716:1720	arg1	cows					1722:1725	lactating dairy cows	1706:1725	lactating dairy cows	1706:1725	Supplementing live yeast to lactating dairy cows did not affect dry matter intake (26.0 kg/d), milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments).
31128871	5	106	theme	Total-tract	1057:1067	arg1	digestibility					1078:1090	Total-tract nutrient digestibility	1057:1090	Total-tract nutrient digestibility	1057:1090	Total-tract nutrient digestibility was estimated using 240-h undigested neutral detergent fiber (NDF) as an internal marker.
31128871	9	107	theme	protein	1862:1868	arg1	concentration					1870:1882	milk protein concentration	1857:1882	milk protein concentration (2.96%)	1857:1890	Supplementing live yeast to lactating dairy cows did not affect dry matter intake (26.0 kg/d), milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments).
31128871	9	107	theme	protein	1862:1868	arg1	%					1889:1889	2.96%	1885:1889	2.96%	1885:1889	Supplementing live yeast to lactating dairy cows did not affect dry matter intake (26.0 kg/d), milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments).
31128871	12	108	theme	potential	2415:2423	arg1	interaction					2425:2435	A potential interaction	2413:2435	A potential interaction	2413:2435	A potential interaction between live-yeast supplementation and NDF passage rate, which may have hindered the beneficial effects of live-yeast supplementation on production performance and nutrient utilization, deserves further research.
31128871	9	109	dep	intake	1753:1758	arg1	units					2092:2096	milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units	1773:2096	milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments	1773:2130	Supplementing live yeast to lactating dairy cows did not affect dry matter intake (26.0 kg/d), milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments).
31128871	2	110	theme	body	460:463	arg1	BW					473:474	BW	473:474	BW	473:474	Eight primiparous [mean ± SD; 569 ± 35 kg of body weight (BW) and 80 ± 29 d in milk (DIM) at the beginning of the experiment] and 16 multiparous (665 ± 67 kg of BW and 64 ± 10 DIM at the beginning of the experiment) Holstein cows were blocked by parity and DIM, and randomly assigned to 1 of 2 diets (control vs. yeast) for a 12-wk-long period according to randomized complete block design.
31128871	2	110	theme	body	460:463	arg1	weight					465:470	body weight	460:470	body weight (BW)	460:475	Eight primiparous [mean ± SD; 569 ± 35 kg of body weight (BW) and 80 ± 29 d in milk (DIM) at the beginning of the experiment] and 16 multiparous (665 ± 67 kg of BW and 64 ± 10 DIM at the beginning of the experiment) Holstein cows were blocked by parity and DIM, and randomly assigned to 1 of 2 diets (control vs. yeast) for a 12-wk-long period according to randomized complete block design.
31128871	2	111	theme	complete	783:790	arg1	block					792:796	complete block	783:796	randomized complete block design	772:803	Eight primiparous [mean ± SD; 569 ± 35 kg of body weight (BW) and 80 ± 29 d in milk (DIM) at the beginning of the experiment] and 16 multiparous (665 ± 67 kg of BW and 64 ± 10 DIM at the beginning of the experiment) Holstein cows were blocked by parity and DIM, and randomly assigned to 1 of 2 diets (control vs. yeast) for a 12-wk-long period according to randomized complete block design.
31128871	4	112	theme	×	954:954	arg1	cfu/d					961:965	approximately 5.4 × 1011 cfu/d	936:965	approximately 5.4 × 1011 cfu/d of Saccharomyces cerevisiae (BeneSacc; Global Nutritech Biotechnology LLC, Richmond, VA)	936:1054	The yeast diet was formulated to provide approximately 5.4 × 1011 cfu/d of Saccharomyces cerevisiae (BeneSacc; Global Nutritech Biotechnology LLC, Richmond, VA).
31128871	9	113	theme	body	2026:2029	arg1	gain					2038:2041	body weight gain	2026:2041	body weight gain (0.62 kg/d)	2026:2053	Supplementing live yeast to lactating dairy cows did not affect dry matter intake (26.0 kg/d), milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments).
31128871	9	113	theme	body	2026:2029	arg1	kg/d					2049:2052	0.62 kg/d	2044:2052	0.62 kg/d	2044:2052	Supplementing live yeast to lactating dairy cows did not affect dry matter intake (26.0 kg/d), milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments).
31128871	8	114	theme	body	1534:1537	arg1	gain					1555:1558	body condition score gain	1534:1558	body condition score gain	1534:1558	The statistical model for analyzing BW gain, body condition score gain, and dry matter and nutrient digestibilities included the effects of block, treatment, and the random residual error.
31128871	7	115	theme	residual	1473:1480	arg1	error					1482:1486	the random residual error	1462:1486	the random residual error	1462:1486	The statistical model for these variables included the effects of block, treatment, the block by treatment interaction, week, the treatment by week interaction, and the random residual error.
31128871	4	116	theme	Nutritech	1013:1021	arg1	LLC					1037:1039	Global Nutritech Biotechnology LLC	1006:1039	Global Nutritech Biotechnology LLC	1006:1039	The yeast diet was formulated to provide approximately 5.4 × 1011 cfu/d of Saccharomyces cerevisiae (BeneSacc; Global Nutritech Biotechnology LLC, Richmond, VA).
31128871	9	117	theme	gain	2038:2041	arg1	units					2092:2096	milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units	1773:2096	milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments	1773:2130	Supplementing live yeast to lactating dairy cows did not affect dry matter intake (26.0 kg/d), milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments).
31128871	8	118	theme	score	1549:1553	arg1	gain					1555:1558	body condition score gain	1534:1558	body condition score gain	1534:1558	The statistical model for analyzing BW gain, body condition score gain, and dry matter and nutrient digestibilities included the effects of block, treatment, and the random residual error.
31128871	2	119	dep	multiparous	548:558	arg1	BW					576:577	BW	576:577	BW	576:577	Eight primiparous [mean ± SD; 569 ± 35 kg of body weight (BW) and 80 ± 29 d in milk (DIM) at the beginning of the experiment] and 16 multiparous (665 ± 67 kg of BW and 64 ± 10 DIM at the beginning of the experiment) Holstein cows were blocked by parity and DIM, and randomly assigned to 1 of 2 diets (control vs. yeast) for a 12-wk-long period according to randomized complete block design.
31128871	2	119	dep	multiparous	548:558	arg1	±					586:586	64 ±	583:586	64 ±	583:586	Eight primiparous [mean ± SD; 569 ± 35 kg of body weight (BW) and 80 ± 29 d in milk (DIM) at the beginning of the experiment] and 16 multiparous (665 ± 67 kg of BW and 64 ± 10 DIM at the beginning of the experiment) Holstein cows were blocked by parity and DIM, and randomly assigned to 1 of 2 diets (control vs. yeast) for a 12-wk-long period according to randomized complete block design.
31128871	2	119	dep	multiparous	548:558	arg1	kg					570:571	kg	570:571	kg	570:571	Eight primiparous [mean ± SD; 569 ± 35 kg of body weight (BW) and 80 ± 29 d in milk (DIM) at the beginning of the experiment] and 16 multiparous (665 ± 67 kg of BW and 64 ± 10 DIM at the beginning of the experiment) Holstein cows were blocked by parity and DIM, and randomly assigned to 1 of 2 diets (control vs. yeast) for a 12-wk-long period according to randomized complete block design.
31128871	9	120	theme	matter	1746:1751	arg1	intake					1753:1758	dry matter intake	1742:1758	dry matter intake (26.0 kg/d), milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments)	1742:2131	Supplementing live yeast to lactating dairy cows did not affect dry matter intake (26.0 kg/d), milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments).
31128871	9	120	theme	matter	1746:1751	arg1	kg/d					1766:1769	26.0 kg/d	1761:1769	26.0 kg/d	1761:1769	Supplementing live yeast to lactating dairy cows did not affect dry matter intake (26.0 kg/d), milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments).
31128871	8	121	theme	statistical	1493:1503	arg1	model					1505:1509	The statistical model	1489:1509	The statistical model for analyzing BW gain, body condition score gain, and dry matter and nutrient digestibilities	1489:1603	The statistical model for analyzing BW gain, body condition score gain, and dry matter and nutrient digestibilities included the effects of block, treatment, and the random residual error.
31128871	5	122	theme	240-h	1112:1116	arg1	NDF					1154:1156	NDF	1154:1156	NDF	1154:1156	Total-tract nutrient digestibility was estimated using 240-h undigested neutral detergent fiber (NDF) as an internal marker.
31128871	5	122	theme	240-h	1112:1116	arg1	fiber					1147:1151	240-h undigested neutral detergent fiber	1112:1151	240-h undigested neutral detergent fiber (NDF)	1112:1157	Total-tract nutrient digestibility was estimated using 240-h undigested neutral detergent fiber (NDF) as an internal marker.
31128871	5	122	theme	240-h	1112:1116	arg1	marker					1174:1179	an internal marker	1162:1179	an internal marker	1162:1179	Total-tract nutrient digestibility was estimated using 240-h undigested neutral detergent fiber (NDF) as an internal marker.
31128871	11	123	theme	high-producing	2386:2399	arg1	cows					2407:2410	high-producing dairy cows	2386:2410	high-producing dairy cows	2386:2410	In conclusion, the supplementation of the live yeast did not affect production performance and nutrient digestibility of high-producing dairy cows.
31128871	5	124	theme	neutral	1129:1135	arg1	NDF					1154:1156	NDF	1154:1156	NDF	1154:1156	Total-tract nutrient digestibility was estimated using 240-h undigested neutral detergent fiber (NDF) as an internal marker.
31128871	5	124	theme	neutral	1129:1135	arg1	fiber					1147:1151	240-h undigested neutral detergent fiber	1112:1151	240-h undigested neutral detergent fiber (NDF)	1112:1157	Total-tract nutrient digestibility was estimated using 240-h undigested neutral detergent fiber (NDF) as an internal marker.
31128871	5	124	theme	neutral	1129:1135	arg1	marker					1174:1179	an internal marker	1162:1179	an internal marker	1162:1179	Total-tract nutrient digestibility was estimated using 240-h undigested neutral detergent fiber (NDF) as an internal marker.
31128871	9	125	theme	protein	1898:1904	arg1	yield					1906:1910	milk protein yield	1893:1910	milk protein yield (1.43 kg/d)	1893:1922	Supplementing live yeast to lactating dairy cows did not affect dry matter intake (26.0 kg/d), milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments).
31128871	9	125	theme	protein	1898:1904	arg1	kg/d					1918:1921	1.43 kg/d	1913:1921	1.43 kg/d	1913:1921	Supplementing live yeast to lactating dairy cows did not affect dry matter intake (26.0 kg/d), milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments).
31128871	1	126	theme	high-producing	368:381	arg1	cows					383:386	high-producing cows	368:386	high-producing cows	368:386	We aimed to evaluate the use of a live-yeast product as a means to attenuate plausible nutritional disturbances when feeding relatively low-forage diets containing rapidly fermentable carbohydrates (i.e., wheat) to high-producing cows in early to mid lactation.
31128871	2	127	dep	SD	441:442	arg1	BW					473:474	BW	473:474	BW	473:474	Eight primiparous [mean ± SD; 569 ± 35 kg of body weight (BW) and 80 ± 29 d in milk (DIM) at the beginning of the experiment] and 16 multiparous (665 ± 67 kg of BW and 64 ± 10 DIM at the beginning of the experiment) Holstein cows were blocked by parity and DIM, and randomly assigned to 1 of 2 diets (control vs. yeast) for a 12-wk-long period according to randomized complete block design.
31128871	2	127	dep	SD	441:442	arg1	kg					454:455	kg	454:455	kg	454:455	Eight primiparous [mean ± SD; 569 ± 35 kg of body weight (BW) and 80 ± 29 d in milk (DIM) at the beginning of the experiment] and 16 multiparous (665 ± 67 kg of BW and 64 ± 10 DIM at the beginning of the experiment) Holstein cows were blocked by parity and DIM, and randomly assigned to 1 of 2 diets (control vs. yeast) for a 12-wk-long period according to randomized complete block design.
31128871	2	127	dep	SD	441:442	arg1	weight					465:470	body weight	460:470	body weight (BW)	460:475	Eight primiparous [mean ± SD; 569 ± 35 kg of body weight (BW) and 80 ± 29 d in milk (DIM) at the beginning of the experiment] and 16 multiparous (665 ± 67 kg of BW and 64 ± 10 DIM at the beginning of the experiment) Holstein cows were blocked by parity and DIM, and randomly assigned to 1 of 2 diets (control vs. yeast) for a 12-wk-long period according to randomized complete block design.
31128871	2	127	dep	SD	441:442	arg1	±					484:484	80 ±	481:484	80 ±	481:484	Eight primiparous [mean ± SD; 569 ± 35 kg of body weight (BW) and 80 ± 29 d in milk (DIM) at the beginning of the experiment] and 16 multiparous (665 ± 67 kg of BW and 64 ± 10 DIM at the beginning of the experiment) Holstein cows were blocked by parity and DIM, and randomly assigned to 1 of 2 diets (control vs. yeast) for a 12-wk-long period according to randomized complete block design.
31128871	8	128	theme	dry	1565:1567	arg1	matter					1569:1574	dry matter	1565:1574	dry matter	1565:1574	The statistical model for analyzing BW gain, body condition score gain, and dry matter and nutrient digestibilities included the effects of block, treatment, and the random residual error.
31128871	7	129	theme	week	1440:1443	arg1	interaction					1445:1455	week interaction	1440:1455	week interaction	1440:1455	The statistical model for these variables included the effects of block, treatment, the block by treatment interaction, week, the treatment by week interaction, and the random residual error.
31128871	8	130	theme	random	1655:1660	arg1	error					1671:1675	the random residual error	1651:1675	the random residual error	1651:1675	The statistical model for analyzing BW gain, body condition score gain, and dry matter and nutrient digestibilities included the effects of block, treatment, and the random residual error.
31128871	0	131	theme	nutrient	27:34	arg1	digestibility					36:48	nutrient digestibility	27:48	nutrient digestibility	27:48	Production performance and nutrient digestibility of lactating dairy cows fed low-forage diets with and without the addition of a live-yeast supplement.
31128871	7	132	theme	treatment	1427:1435	arg1	effects					1352:1358	the effects	1348:1358	the effects of block, treatment, the block by treatment interaction, week, the treatment by week interaction, and the random residual error	1348:1486	The statistical model for these variables included the effects of block, treatment, the block by treatment interaction, week, the treatment by week interaction, and the random residual error.
31128871	2	133	theme	primiparous	421:431	arg1	±					439:439	Eight primiparous [mean ±	415:439	Eight primiparous [mean ± SD; 569 ± 35 kg of body weight (BW) and 80 ± 29 d in milk (DIM) at the beginning of the experiment] and 16 multiparous (665 ± 67 kg of BW and 64 ± 10 DIM at the beginning of the experiment) Holstein cows	415:643	Eight primiparous [mean ± SD; 569 ± 35 kg of body weight (BW) and 80 ± 29 d in milk (DIM) at the beginning of the experiment] and 16 multiparous (665 ± 67 kg of BW and 64 ± 10 DIM at the beginning of the experiment) Holstein cows were blocked by parity and DIM, and randomly assigned to 1 of 2 diets (control vs. yeast) for a 12-wk-long period according to randomized complete block design.
31128871	2	134	from	beginning	512:520	arg1	milk					494:497	milk	494:497	milk (DIM) at the beginning of the experiment]	494:539	Eight primiparous [mean ± SD; 569 ± 35 kg of body weight (BW) and 80 ± 29 d in milk (DIM) at the beginning of the experiment] and 16 multiparous (665 ± 67 kg of BW and 64 ± 10 DIM at the beginning of the experiment) Holstein cows were blocked by parity and DIM, and randomly assigned to 1 of 2 diets (control vs. yeast) for a 12-wk-long period according to randomized complete block design.
31128871	2	134	from	beginning	512:520	arg1	DIM					500:502	DIM	500:502	DIM	500:502	Eight primiparous [mean ± SD; 569 ± 35 kg of body weight (BW) and 80 ± 29 d in milk (DIM) at the beginning of the experiment] and 16 multiparous (665 ± 67 kg of BW and 64 ± 10 DIM at the beginning of the experiment) Holstein cows were blocked by parity and DIM, and randomly assigned to 1 of 2 diets (control vs. yeast) for a 12-wk-long period according to randomized complete block design.
31128871	6	135	theme	intake	1193:1198	arg1	concentrations					1232:1245	Dry matter intake, milk yield, and milk component concentrations	1182:1245	Dry matter intake, milk yield, and milk component concentrations	1182:1245	Dry matter intake, milk yield, and milk component concentrations and yields were analyzed using repeated measures.
31128871	1	136	theme	low-forage	289:298	arg1	diets					300:304	relatively low-forage diets	278:304	relatively low-forage diets containing rapidly fermentable carbohydrates (i.e., wheat) to high-producing cows in early to mid lactation	278:412	We aimed to evaluate the use of a live-yeast product as a means to attenuate plausible nutritional disturbances when feeding relatively low-forage diets containing rapidly fermentable carbohydrates (i.e., wheat) to high-producing cows in early to mid lactation.
31128871	9	137	theme	condition	2065:2073	arg1	gain					2081:2084	body condition score gain	2060:2084	body condition score gain	2060:2084	Supplementing live yeast to lactating dairy cows did not affect dry matter intake (26.0 kg/d), milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments).
31128871	9	138	theme	yield	1778:1782	arg1	units					2092:2096	milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units	1773:2096	milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments	1773:2130	Supplementing live yeast to lactating dairy cows did not affect dry matter intake (26.0 kg/d), milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments).
31128871	6	139	theme	Dry	1182:1184	arg1	concentrations					1232:1245	Dry matter intake, milk yield, and milk component concentrations	1182:1245	Dry matter intake, milk yield, and milk component concentrations	1182:1245	Dry matter intake, milk yield, and milk component concentrations and yields were analyzed using repeated measures.
31128871	9	140	theme	gain	2081:2084	arg1	yield					1778:1782	milk yield	1773:1782	milk yield (48.1 kg/d)	1773:1794	Supplementing live yeast to lactating dairy cows did not affect dry matter intake (26.0 kg/d), milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments).
31128871	9	140	theme	gain	2081:2084	arg1	kg/d					1790:1793	48.1 kg/d	1785:1793	48.1 kg/d	1785:1793	Supplementing live yeast to lactating dairy cows did not affect dry matter intake (26.0 kg/d), milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments).
31128871	7	141	theme	statistical	1301:1311	arg1	model					1313:1317	The statistical model	1297:1317	The statistical model for these variables	1297:1337	The statistical model for these variables included the effects of block, treatment, the block by treatment interaction, week, the treatment by week interaction, and the random residual error.
31128871	6	142	theme	yield	1206:1210	arg1	concentrations					1232:1245	Dry matter intake, milk yield, and milk component concentrations	1182:1245	Dry matter intake, milk yield, and milk component concentrations	1182:1245	Dry matter intake, milk yield, and milk component concentrations and yields were analyzed using repeated measures.
31128871	3	143	contain	contained	827:835	arg2	%					880:880	55%	878:880	55% concentrate	878:892	The formulated diets contained 36.7% corn silage, 8.3% alfalfa hay, and 55% concentrate.
31128871	3	143	contain	contained	827:835	arg1	diets					821:825	The formulated diets	806:825	The formulated diets	806:825	The formulated diets contained 36.7% corn silage, 8.3% alfalfa hay, and 55% concentrate.
31128871	3	143	contain	contained	827:835	arg2	%					859:859	8.3% alfalfa	856:867	8.3% alfalfa hay	856:871	The formulated diets contained 36.7% corn silage, 8.3% alfalfa hay, and 55% concentrate.
31128871	3	143	contain	contained	827:835	arg2	%					841:841	36.7%	837:841	36.7% corn silage	837:853	The formulated diets contained 36.7% corn silage, 8.3% alfalfa hay, and 55% concentrate.
31128871	1	144	dep	wheat	358:362	arg1	i.e.					352:355	i.e.	352:355	i.e.	352:355	We aimed to evaluate the use of a live-yeast product as a means to attenuate plausible nutritional disturbances when feeding relatively low-forage diets containing rapidly fermentable carbohydrates (i.e., wheat) to high-producing cows in early to mid lactation.
31128871	12	145	theme	live-yeast	2544:2553	arg1	supplementation					2555:2569	live-yeast supplementation	2544:2569	live-yeast supplementation	2544:2569	A potential interaction between live-yeast supplementation and NDF passage rate, which may have hindered the beneficial effects of live-yeast supplementation on production performance and nutrient utilization, deserves further research.
31128871	5	146	theme	internal	1165:1172	arg1	fiber					1147:1151	240-h undigested neutral detergent fiber	1112:1151	240-h undigested neutral detergent fiber (NDF)	1112:1157	Total-tract nutrient digestibility was estimated using 240-h undigested neutral detergent fiber (NDF) as an internal marker.
31128871	5	146	theme	internal	1165:1172	arg1	marker					1174:1179	an internal marker	1162:1179	an internal marker	1162:1179	Total-tract nutrient digestibility was estimated using 240-h undigested neutral detergent fiber (NDF) as an internal marker.
31128871	9	147	theme	lactose	1930:1936	arg1	concentration					1938:1950	milk lactose concentration	1925:1950	milk lactose concentration (4.84%)	1925:1958	Supplementing live yeast to lactating dairy cows did not affect dry matter intake (26.0 kg/d), milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments).
31128871	9	147	theme	lactose	1930:1936	arg1	%					1957:1957	4.84%	1953:1957	4.84%	1953:1957	Supplementing live yeast to lactating dairy cows did not affect dry matter intake (26.0 kg/d), milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments).
31128871	1	148	theme	fermentable	325:335	arg1	carbohydrates					337:349	rapidly fermentable carbohydrates	317:349	rapidly fermentable carbohydrates (i.e., wheat) to high-producing cows	317:386	We aimed to evaluate the use of a live-yeast product as a means to attenuate plausible nutritional disturbances when feeding relatively low-forage diets containing rapidly fermentable carbohydrates (i.e., wheat) to high-producing cows in early to mid lactation.
31128871	11	149	theme	yeast	2312:2316	arg1	supplementation					2284:2298	the supplementation	2280:2298	the supplementation of the live yeast	2280:2316	In conclusion, the supplementation of the live yeast did not affect production performance and nutrient digestibility of high-producing dairy cows.
31128871	9	150	theme	urea	1998:2001	arg1	nitrogen					2003:2010	milk urea nitrogen	1993:2010	milk urea nitrogen (7.99 mg/dL)	1993:2023	Supplementing live yeast to lactating dairy cows did not affect dry matter intake (26.0 kg/d), milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments).
31128871	9	150	theme	urea	1998:2001	arg1	mg/dL					2018:2022	7.99 mg/dL	2013:2022	7.99 mg/dL	2013:2022	Supplementing live yeast to lactating dairy cows did not affect dry matter intake (26.0 kg/d), milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments).
31128871	12	151	theme	nutrient	2601:2608	arg1	utilization					2610:2620	nutrient utilization	2601:2620	nutrient utilization	2601:2620	A potential interaction between live-yeast supplementation and NDF passage rate, which may have hindered the beneficial effects of live-yeast supplementation on production performance and nutrient utilization, deserves further research.
31128871	2	152	dep	milk	494:497	arg1	cows					640:643	Holstein cows	631:643	Holstein cows	631:643	Eight primiparous [mean ± SD; 569 ± 35 kg of body weight (BW) and 80 ± 29 d in milk (DIM) at the beginning of the experiment] and 16 multiparous (665 ± 67 kg of BW and 64 ± 10 DIM at the beginning of the experiment) Holstein cows were blocked by parity and DIM, and randomly assigned to 1 of 2 diets (control vs. yeast) for a 12-wk-long period according to randomized complete block design.
31128871	9	153	theme	milk	1797:1800	arg1	concentration					1806:1818	milk fat concentration	1797:1818	milk fat concentration (3.61%)	1797:1826	Supplementing live yeast to lactating dairy cows did not affect dry matter intake (26.0 kg/d), milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments).
31128871	9	153	theme	milk	1797:1800	arg1	%					1825:1825	3.61%	1821:1825	3.61%	1821:1825	Supplementing live yeast to lactating dairy cows did not affect dry matter intake (26.0 kg/d), milk yield (48.1 kg/d), milk fat concentration (3.61%), milk fat yield (1.72 kg/d), milk protein concentration (2.96%), milk protein yield (1.43 kg/d), milk lactose concentration (4.84%), milk lactose yield (2.35 kg/d), milk urea nitrogen (7.99 mg/dL), body weight gain (0.62 kg/d), and body condition score gain (0.02 units; all averages of the 2 treatments).
31128871	10	154	theme	dry	2157:2159	arg1	matter					2161:2166	dry matter	2157:2166	dry matter (70.2%)	2157:2174	The digestibilities of dry matter (70.2%), crude protein (71.4%), NDF (36.4%), and starch (99.8%) were not affected by treatments.
31128871	10	154	theme	dry	2157:2159	arg1	%					2173:2173	70.2%	2169:2173	70.2%	2169:2173	The digestibilities of dry matter (70.2%), crude protein (71.4%), NDF (36.4%), and starch (99.8%) were not affected by treatments.
31128871	3	155	dep	%	841:841	arg1	silage					848:853	corn silage	843:853	36.7% corn silage	837:853	The formulated diets contained 36.7% corn silage, 8.3% alfalfa hay, and 55% concentrate.
29981947	8	0	theme	wash	1170:1173	arg1	paradigm					1175:1182	This wash paradigm	1165:1182	This wash paradigm	1165:1182	This wash paradigm did not significantly affect collagen or glycosaminoglycan content, but it was sufficient to remove any trace of the cytotoxic compound based on conditioned media experiments.
29981947	7	1	theme	DNase	1148:1152	arg1	treatment					1154:1162	DNase treatment	1148:1162	DNase treatment	1148:1162	Clearance of the cellular components was then achieved in an apoptosis-dependent manner using a gentle wash in hypertonic phosphate buffered saline followed by DNase treatment.
29981947	9	2	theme	intact	1452:1457	arg1	lamina					1465:1470	an intact basal lamina	1449:1470	an intact basal lamina microarchitecture mimicking that of native, unprocessed nerve	1449:1532	The resulting acellular tissue graft was immunogenically tolerated in vivo and exhibited an intact basal lamina microarchitecture mimicking that of native, unprocessed nerve.
29981947	12	3	theme	pro-regenerative	2086:2101	arg1	effects					2103:2109	the pro-regenerative effects	2082:2109	the pro-regenerative effects	2082:2109	Current technologies typically rely on the use of harsh agents for clearing cellular debris, altering the tissue structure and potentially diminishing the pro-regenerative effects.
29981947	2	4	theme	cellular	308:315	arg1	components					317:326	cellular components	308:326	cellular components	308:326	Many current protocols nonetheless rely on detergents that aid extraction of cellular components but also damage tissue architecture.
29981947	9	5	theme	lamina	1465:1470	arg1	microarchitecture					1472:1488	an intact basal lamina microarchitecture	1449:1488	an intact basal lamina microarchitecture mimicking that of native, unprocessed nerve	1449:1532	The resulting acellular tissue graft was immunogenically tolerated in vivo and exhibited an intact basal lamina microarchitecture mimicking that of native, unprocessed nerve.
29981947	7	6	theme	buffered	1120:1127	arg1	saline					1129:1134	hypertonic phosphate buffered saline	1099:1134	hypertonic phosphate buffered saline followed by DNase treatment	1099:1162	Clearance of the cellular components was then achieved in an apoptosis-dependent manner using a gentle wash in hypertonic phosphate buffered saline followed by DNase treatment.
29981947	6	7	theme	peripheral	815:824	arg1	tissue					832:837	peripheral nerve tissue	815:837	peripheral nerve tissue	815:837	We have demonstrated that treating peripheral nerve tissue with camptothecin induced both an early marker of apoptosis, cleaved caspase-3 expression, as well as a late stage marker, TUNEL+ DNA fragmentation.
29981947	1	8	theme	tissue	187:192	arg1	methods					222:228	tissue and organ decellularization methods	187:228	tissue and organ decellularization methods	187:228	Preservation of tissue structure is often a primary goal when optimizing tissue and organ decellularization methods.
29981947	8	9	theme	collagen	1213:1220	arg1	content					1243:1249	collagen or glycosaminoglycan content	1213:1249	content	1243:1249	This wash paradigm did not significantly affect collagen or glycosaminoglycan content, but it was sufficient to remove any trace of the cytotoxic compound based on conditioned media experiments.
29981947	6	10	theme	cleaved	900:906	arg1	expression					918:927	cleaved caspase-3 expression	900:927	cleaved caspase-3 expression	900:927	We have demonstrated that treating peripheral nerve tissue with camptothecin induced both an early marker of apoptosis, cleaved caspase-3 expression, as well as a late stage marker, TUNEL+ DNA fragmentation.
29981947	7	11	theme	hypertonic	1099:1108	arg1	saline					1129:1134	hypertonic phosphate buffered saline	1099:1134	hypertonic phosphate buffered saline followed by DNase treatment	1099:1162	Clearance of the cellular components was then achieved in an apoptosis-dependent manner using a gentle wash in hypertonic phosphate buffered saline followed by DNase treatment.
29981947	14	12	theme	hypertonic	2436:2445	arg1	buffers					2452:2458	only hypertonic wash buffers	2431:2458	only hypertonic wash buffers	2431:2458	The novelty of this process is in the induction of programmed cell death - or apoptosis - via a general cytotoxin, thereby enabling antigen clearance using only hypertonic wash buffers.
29981947	1	13	theme	organ	198:202	arg1	methods					222:228	tissue and organ decellularization methods	187:228	tissue and organ decellularization methods	187:228	Preservation of tissue structure is often a primary goal when optimizing tissue and organ decellularization methods.
29981947	8	14	theme	glycosaminoglycan	1225:1241	arg1	content					1243:1249	collagen or glycosaminoglycan content	1213:1249	content	1243:1249	This wash paradigm did not significantly affect collagen or glycosaminoglycan content, but it was sufficient to remove any trace of the cytotoxic compound based on conditioned media experiments.
29981947	11	15	theme	organ	1870:1874	arg1	replacements					1876:1887	non-immunogenic organ replacements	1854:1887	non-immunogenic organ replacements for a broad range of health applications	1854:1928	STATEMENT OF SIGNIFICANCE Tissue decellularization has expanded the ability to generate non-immunogenic organ replacements for a broad range of health applications.
29981947	13	16	theme	nerve	2213:2217	arg1	tissue					2219:2224	peripheral nerve tissue	2202:2224	peripheral nerve tissue	2202:2224	We have developed a method for effectively, yet gently, removing cellular components from peripheral nerve tissue while preserving the native tissue architecture.
29981947	6	17	theme	TUNEL+	962:967	arg1	marker					954:959	a late stage marker	941:959	a late stage marker	941:959	We have demonstrated that treating peripheral nerve tissue with camptothecin induced both an early marker of apoptosis, cleaved caspase-3 expression, as well as a late stage marker, TUNEL+ DNA fragmentation.
29981947	6	17	theme	TUNEL+	962:967	arg1	fragmentation					973:985	TUNEL+ DNA fragmentation	962:985	TUNEL+ DNA fragmentation	962:985	We have demonstrated that treating peripheral nerve tissue with camptothecin induced both an early marker of apoptosis, cleaved caspase-3 expression, as well as a late stage marker, TUNEL+ DNA fragmentation.
29981947	13	18	theme	native	2247:2252	arg1	architecture					2261:2272	the native tissue architecture	2243:2272	the native tissue architecture	2243:2272	We have developed a method for effectively, yet gently, removing cellular components from peripheral nerve tissue while preserving the native tissue architecture.
29981947	15	19	theme	low	2578:2580	arg1	responses					2589:2597	low immune responses	2578:2597	low immune responses comparable to an isograft negative control in a model of subcutaneous implantation	2578:2680	The resulting acellular nerve scaffolds are nearly identical to unprocessed tissue on a microscopic level and elicit low immune responses comparable to an isograft negative control in a model of subcutaneous implantation.
29981947	12	20	theme	tissue	2037:2042	arg1	structure					2044:2052	the tissue structure	2033:2052	the tissue structure	2033:2052	Current technologies typically rely on the use of harsh agents for clearing cellular debris, altering the tissue structure and potentially diminishing the pro-regenerative effects.
29981947	8	21	theme	compound	1311:1318	arg1	trace					1288:1292	any trace	1284:1292	any trace of the cytotoxic compound based on conditioned media experiments	1284:1357	This wash paradigm did not significantly affect collagen or glycosaminoglycan content, but it was sufficient to remove any trace of the cytotoxic compound based on conditioned media experiments.
29981947	10	22	theme	reagents	1644:1651	arg1	use					1631:1633	the use	1627:1633	the use of harsh reagents	1627:1651	Hence, ex vivo induction of apoptosis is a promising method to decellularize tissue without the use of harsh reagents while better preserving the benefits of native tissue such as tissue-specific composition and microarchitecture.
29981947	11	23	theme	broad	1895:1899	arg1	range					1901:1905	a broad range	1893:1905	a broad range of health applications	1893:1928	STATEMENT OF SIGNIFICANCE Tissue decellularization has expanded the ability to generate non-immunogenic organ replacements for a broad range of health applications.
29981947	13	24	theme	cellular	2177:2184	arg1	components					2186:2195	cellular components	2177:2195	cellular components	2177:2195	We have developed a method for effectively, yet gently, removing cellular components from peripheral nerve tissue while preserving the native tissue architecture.
29981947	2	25	theme	current	236:242	arg1	protocols					244:252	Many current protocols	231:252	Many current protocols	231:252	Many current protocols nonetheless rely on detergents that aid extraction of cellular components but also damage tissue architecture.
29981947	7	26	theme	components	1014:1023	arg1	Clearance					988:996	Clearance	988:996	Clearance of the cellular components	988:1023	Clearance of the cellular components was then achieved in an apoptosis-dependent manner using a gentle wash in hypertonic phosphate buffered saline followed by DNase treatment.
29981947	1	27	theme	structure	137:145	arg1	goal					166:169	a primary goal	156:169	a primary goal when optimizing tissue and organ decellularization methods	156:228	Preservation of tissue structure is often a primary goal when optimizing tissue and organ decellularization methods.
29981947	1	27	theme	structure	137:145	arg1	Preservation					114:125	Preservation	114:125	Preservation of tissue structure	114:145	Preservation of tissue structure is often a primary goal when optimizing tissue and organ decellularization methods.
29981947	6	28	theme	late	943:946	arg1	marker					954:959	a late stage marker	941:959	a late stage marker	941:959	We have demonstrated that treating peripheral nerve tissue with camptothecin induced both an early marker of apoptosis, cleaved caspase-3 expression, as well as a late stage marker, TUNEL+ DNA fragmentation.
29981947	6	28	theme	late	943:946	arg1	fragmentation					973:985	TUNEL+ DNA fragmentation	962:985	TUNEL+ DNA fragmentation	962:985	We have demonstrated that treating peripheral nerve tissue with camptothecin induced both an early marker of apoptosis, cleaved caspase-3 expression, as well as a late stage marker, TUNEL+ DNA fragmentation.
29981947	15	29	theme	microscopic	2549:2559	arg1	level					2561:2565	a microscopic level	2547:2565	a microscopic level	2547:2565	The resulting acellular nerve scaffolds are nearly identical to unprocessed tissue on a microscopic level and elicit low immune responses comparable to an isograft negative control in a model of subcutaneous implantation.
29981947	8	30	theme	media	1341:1345	arg1	experiments					1347:1357	conditioned media experiments	1329:1357	conditioned media experiments	1329:1357	This wash paradigm did not significantly affect collagen or glycosaminoglycan content, but it was sufficient to remove any trace of the cytotoxic compound based on conditioned media experiments.
29981947	11	31	theme	decellularization	1799:1815	arg1	STATEMENT					1766:1774	STATEMENT	1766:1774	STATEMENT OF SIGNIFICANCE Tissue decellularization	1766:1815	STATEMENT OF SIGNIFICANCE Tissue decellularization has expanded the ability to generate non-immunogenic organ replacements for a broad range of health applications.
29981947	13	32	theme	tissue	2254:2259	arg1	architecture					2261:2272	the native tissue architecture	2243:2272	the native tissue architecture	2243:2272	We have developed a method for effectively, yet gently, removing cellular components from peripheral nerve tissue while preserving the native tissue architecture.
29981947	15	33	theme	unprocessed	2525:2535	arg1	tissue					2537:2542	unprocessed tissue	2525:2542	unprocessed tissue on a microscopic level	2525:2565	The resulting acellular nerve scaffolds are nearly identical to unprocessed tissue on a microscopic level and elicit low immune responses comparable to an isograft negative control in a model of subcutaneous implantation.
29981947	1	34	theme	primary	158:164	arg1	Preservation					114:125	Preservation	114:125	Preservation of tissue structure	114:145	Preservation of tissue structure is often a primary goal when optimizing tissue and organ decellularization methods.
29981947	1	34	theme	primary	158:164	arg1	goal					166:169	a primary goal	156:169	a primary goal when optimizing tissue and organ decellularization methods	156:228	Preservation of tissue structure is often a primary goal when optimizing tissue and organ decellularization methods.
29981947	15	35	from	control	2634:2640	arg1	model					2647:2651	a model	2645:2651	a model of subcutaneous implantation	2645:2680	The resulting acellular nerve scaffolds are nearly identical to unprocessed tissue on a microscopic level and elicit low immune responses comparable to an isograft negative control in a model of subcutaneous implantation.
29981947	0	36	theme	nerve	90:94	arg1	structure					103:111	nerve tissue structure	90:111	nerve tissue structure	90:111	Development of an apoptosis-assisted decellularization method for maximal preservation of nerve tissue structure.
29981947	14	37	theme	general	2371:2377	arg1	cytotoxin					2379:2387	a general cytotoxin	2369:2387	a general cytotoxin	2369:2387	The novelty of this process is in the induction of programmed cell death - or apoptosis - via a general cytotoxin, thereby enabling antigen clearance using only hypertonic wash buffers.
29981947	0	38	theme	structure	103:111	arg1	preservation					74:85	maximal preservation	66:85	maximal preservation of nerve tissue structure	66:111	Development of an apoptosis-assisted decellularization method for maximal preservation of nerve tissue structure.
29981947	12	39	theme	cellular	2007:2014	arg1	debris					2016:2021	cellular debris	2007:2021	cellular debris	2007:2021	Current technologies typically rely on the use of harsh agents for clearing cellular debris, altering the tissue structure and potentially diminishing the pro-regenerative effects.
29981947	15	40	theme	isograft	2616:2623	arg1	control					2634:2640	an isograft negative control	2613:2640	an isograft negative control in a model of subcutaneous implantation	2613:2680	The resulting acellular nerve scaffolds are nearly identical to unprocessed tissue on a microscopic level and elicit low immune responses comparable to an isograft negative control in a model of subcutaneous implantation.
29981947	3	41	theme	cell	460:463	arg1	removal					465:471	cell removal	460:471	cell removal	460:471	It may be more beneficial to leverage an innate cellular process such as apoptosis and promote cell removal without the use of damaging reagents.
29981947	5	42	theme	wash	766:769	arg1	buffers					771:777	only mild wash buffers	756:777	only mild wash buffers	756:777	We have developed a method that leverages this process to achieve tissue decellularization using only mild wash buffers.
29981947	15	43	theme	nerve	2485:2489	arg1	scaffolds					2491:2499	The resulting acellular nerve scaffolds	2461:2499	The resulting acellular nerve scaffolds	2461:2499	The resulting acellular nerve scaffolds are nearly identical to unprocessed tissue on a microscopic level and elicit low immune responses comparable to an isograft negative control in a model of subcutaneous implantation.
29981947	15	43	theme	nerve	2485:2489	arg1	identical					2512:2520	identical	2512:2520	identical	2512:2520	The resulting acellular nerve scaffolds are nearly identical to unprocessed tissue on a microscopic level and elicit low immune responses comparable to an isograft negative control in a model of subcutaneous implantation.
29981947	3	44	theme	innate	406:411	arg1	process					422:428	an innate cellular process	403:428	an innate cellular process	403:428	It may be more beneficial to leverage an innate cellular process such as apoptosis and promote cell removal without the use of damaging reagents.
29981947	14	45	theme	apoptosis	2353:2361	arg1	induction					2313:2321	the induction	2309:2321	the induction of programmed cell death - or apoptosis - via a general cytotoxin	2309:2387	The novelty of this process is in the induction of programmed cell death - or apoptosis - via a general cytotoxin, thereby enabling antigen clearance using only hypertonic wash buffers.
29981947	12	46	theme	harsh	1981:1985	arg1	agents					1987:1992	harsh agents	1981:1992	harsh agents	1981:1992	Current technologies typically rely on the use of harsh agents for clearing cellular debris, altering the tissue structure and potentially diminishing the pro-regenerative effects.
29981947	15	47	theme	resulting	2465:2473	arg1	scaffolds					2491:2499	The resulting acellular nerve scaffolds	2461:2499	The resulting acellular nerve scaffolds	2461:2499	The resulting acellular nerve scaffolds are nearly identical to unprocessed tissue on a microscopic level and elicit low immune responses comparable to an isograft negative control in a model of subcutaneous implantation.
29981947	15	47	theme	resulting	2465:2473	arg1	identical					2512:2520	identical	2512:2520	identical	2512:2520	The resulting acellular nerve scaffolds are nearly identical to unprocessed tissue on a microscopic level and elicit low immune responses comparable to an isograft negative control in a model of subcutaneous implantation.
29981947	10	48	theme	ex	1542:1543	arg1	induction					1550:1558	ex vivo induction	1542:1558	ex vivo induction of apoptosis	1542:1571	Hence, ex vivo induction of apoptosis is a promising method to decellularize tissue without the use of harsh reagents while better preserving the benefits of native tissue such as tissue-specific composition and microarchitecture.
29981947	10	48	theme	ex	1542:1543	arg1	method					1588:1593	a promising method	1576:1593	a promising method to decellularize tissue without the use of harsh reagents while better preserving the benefits of native tissue such as tissue-specific composition and microarchitecture	1576:1763	Hence, ex vivo induction of apoptosis is a promising method to decellularize tissue without the use of harsh reagents while better preserving the benefits of native tissue such as tissue-specific composition and microarchitecture.
29981947	10	49	theme	tissue-specific	1715:1729	arg1	composition					1731:1741	tissue-specific composition	1715:1741	tissue-specific composition	1715:1741	Hence, ex vivo induction of apoptosis is a promising method to decellularize tissue without the use of harsh reagents while better preserving the benefits of native tissue such as tissue-specific composition and microarchitecture.
29981947	7	50	theme	cellular	1005:1012	arg1	components					1014:1023	the cellular components	1001:1023	the cellular components	1001:1023	Clearance of the cellular components was then achieved in an apoptosis-dependent manner using a gentle wash in hypertonic phosphate buffered saline followed by DNase treatment.
29981947	9	51	theme	acellular	1374:1382	arg1	graft					1391:1395	The resulting acellular tissue graft	1360:1395	The resulting acellular tissue graft	1360:1395	The resulting acellular tissue graft was immunogenically tolerated in vivo and exhibited an intact basal lamina microarchitecture mimicking that of native, unprocessed nerve.
29981947	14	52	theme	cell	2337:2340	arg1	death					2342:2346	programmed cell death	2326:2346	programmed cell death	2326:2346	The novelty of this process is in the induction of programmed cell death - or apoptosis - via a general cytotoxin, thereby enabling antigen clearance using only hypertonic wash buffers.
29981947	0	53	theme	decellularization	37:53	arg1	method					55:60	an apoptosis-assisted decellularization method	15:60	an apoptosis-assisted decellularization method for maximal preservation of nerve tissue structure	15:111	Development of an apoptosis-assisted decellularization method for maximal preservation of nerve tissue structure.
29981947	6	54	dep	marker	879:884	arg1	both					865:868	both	865:868	both	865:868	We have demonstrated that treating peripheral nerve tissue with camptothecin induced both an early marker of apoptosis, cleaved caspase-3 expression, as well as a late stage marker, TUNEL+ DNA fragmentation.
29981947	10	55	theme	apoptosis	1563:1571	arg1	induction					1550:1558	ex vivo induction	1542:1558	ex vivo induction of apoptosis	1542:1571	Hence, ex vivo induction of apoptosis is a promising method to decellularize tissue without the use of harsh reagents while better preserving the benefits of native tissue such as tissue-specific composition and microarchitecture.
29981947	10	55	theme	apoptosis	1563:1571	arg1	method					1588:1593	a promising method	1576:1593	a promising method to decellularize tissue without the use of harsh reagents while better preserving the benefits of native tissue such as tissue-specific composition and microarchitecture	1576:1763	Hence, ex vivo induction of apoptosis is a promising method to decellularize tissue without the use of harsh reagents while better preserving the benefits of native tissue such as tissue-specific composition and microarchitecture.
29981947	6	56	theme	early	873:877	arg1	marker					879:884	an early marker	870:884	an early marker of apoptosis	870:897	We have demonstrated that treating peripheral nerve tissue with camptothecin induced both an early marker of apoptosis, cleaved caspase-3 expression, as well as a late stage marker, TUNEL+ DNA fragmentation.
29981947	11	57	theme	Tissue	1792:1797	arg1	decellularization					1799:1815	SIGNIFICANCE Tissue decellularization	1779:1815	SIGNIFICANCE Tissue decellularization	1779:1815	STATEMENT OF SIGNIFICANCE Tissue decellularization has expanded the ability to generate non-immunogenic organ replacements for a broad range of health applications.
29981947	3	58	theme	damaging	492:499	arg1	reagents					501:508	damaging reagents	492:508	damaging reagents	492:508	It may be more beneficial to leverage an innate cellular process such as apoptosis and promote cell removal without the use of damaging reagents.
29981947	12	59	theme	Current	1931:1937	arg1	technologies					1939:1950	Current technologies	1931:1950	Current technologies	1931:1950	Current technologies typically rely on the use of harsh agents for clearing cellular debris, altering the tissue structure and potentially diminishing the pro-regenerative effects.
29981947	14	60	theme	programmed	2326:2335	arg1	death					2342:2346	programmed cell death	2326:2346	programmed cell death	2326:2346	The novelty of this process is in the induction of programmed cell death - or apoptosis - via a general cytotoxin, thereby enabling antigen clearance using only hypertonic wash buffers.
29981947	9	61	dep	native	1508:1513	arg1	unprocessed					1516:1526	unprocessed	1516:1526	unprocessed	1516:1526	The resulting acellular tissue graft was immunogenically tolerated in vivo and exhibited an intact basal lamina microarchitecture mimicking that of native, unprocessed nerve.
29981947	15	62	theme	subcutaneous	2656:2667	arg1	implantation					2669:2680	subcutaneous implantation	2656:2680	subcutaneous implantation	2656:2680	The resulting acellular nerve scaffolds are nearly identical to unprocessed tissue on a microscopic level and elicit low immune responses comparable to an isograft negative control in a model of subcutaneous implantation.
29981947	4	63	theme	internal	589:596	arg1	components					598:607	its internal components	585:607	its internal components	585:607	During apoptosis, a cell detaches from the extracellular matrix, degrades its internal components, and fragments its contents for easier clearance.
29981947	2	64	theme	components	317:326	arg1	extraction					294:303	extraction	294:303	extraction of cellular components	294:326	Many current protocols nonetheless rely on detergents that aid extraction of cellular components but also damage tissue architecture.
29981947	9	65	theme	basal	1459:1463	arg1	lamina					1465:1470	an intact basal lamina	1449:1470	an intact basal lamina microarchitecture mimicking that of native, unprocessed nerve	1449:1532	The resulting acellular tissue graft was immunogenically tolerated in vivo and exhibited an intact basal lamina microarchitecture mimicking that of native, unprocessed nerve.
29981947	7	66	from	wash	1091:1094	arg1	saline					1129:1134	hypertonic phosphate buffered saline	1099:1134	hypertonic phosphate buffered saline followed by DNase treatment	1099:1162	Clearance of the cellular components was then achieved in an apoptosis-dependent manner using a gentle wash in hypertonic phosphate buffered saline followed by DNase treatment.
29981947	14	67	theme	process	2295:2301	arg1	novelty					2279:2285	The novelty	2275:2285	The novelty of this process	2275:2301	The novelty of this process is in the induction of programmed cell death - or apoptosis - via a general cytotoxin, thereby enabling antigen clearance using only hypertonic wash buffers.
29981947	7	68	theme	phosphate	1110:1118	arg1	saline					1129:1134	hypertonic phosphate buffered saline	1099:1134	hypertonic phosphate buffered saline followed by DNase treatment	1099:1162	Clearance of the cellular components was then achieved in an apoptosis-dependent manner using a gentle wash in hypertonic phosphate buffered saline followed by DNase treatment.
29981947	6	69	theme	nerve	826:830	arg1	tissue					832:837	peripheral nerve tissue	815:837	peripheral nerve tissue	815:837	We have demonstrated that treating peripheral nerve tissue with camptothecin induced both an early marker of apoptosis, cleaved caspase-3 expression, as well as a late stage marker, TUNEL+ DNA fragmentation.
29981947	6	70	theme	caspase-3	908:916	arg1	expression					918:927	cleaved caspase-3 expression	900:927	cleaved caspase-3 expression	900:927	We have demonstrated that treating peripheral nerve tissue with camptothecin induced both an early marker of apoptosis, cleaved caspase-3 expression, as well as a late stage marker, TUNEL+ DNA fragmentation.
29981947	10	71	theme	harsh	1638:1642	arg1	reagents					1644:1651	harsh reagents	1638:1651	harsh reagents	1638:1651	Hence, ex vivo induction of apoptosis is a promising method to decellularize tissue without the use of harsh reagents while better preserving the benefits of native tissue such as tissue-specific composition and microarchitecture.
29981947	14	72	theme	wash	2447:2450	arg1	buffers					2452:2458	only hypertonic wash buffers	2431:2458	only hypertonic wash buffers	2431:2458	The novelty of this process is in the induction of programmed cell death - or apoptosis - via a general cytotoxin, thereby enabling antigen clearance using only hypertonic wash buffers.
29981947	2	73	theme	tissue	344:349	arg1	architecture					351:362	tissue architecture	344:362	tissue architecture	344:362	Many current protocols nonetheless rely on detergents that aid extraction of cellular components but also damage tissue architecture.
29981947	2	74	theme	Many	231:234	arg1	protocols					244:252	Many current protocols	231:252	Many current protocols	231:252	Many current protocols nonetheless rely on detergents that aid extraction of cellular components but also damage tissue architecture.
29981947	7	75	theme	gentle	1084:1089	arg1	wash					1091:1094	a gentle wash	1082:1094	a gentle wash in hypertonic phosphate buffered saline followed by DNase treatment	1082:1162	Clearance of the cellular components was then achieved in an apoptosis-dependent manner using a gentle wash in hypertonic phosphate buffered saline followed by DNase treatment.
29981947	9	76	theme	native	1508:1513	arg1	nerve					1528:1532	native, unprocessed nerve	1508:1532	native, unprocessed nerve	1508:1532	The resulting acellular tissue graft was immunogenically tolerated in vivo and exhibited an intact basal lamina microarchitecture mimicking that of native, unprocessed nerve.
29981947	6	77	theme	DNA	969:971	arg1	marker					954:959	a late stage marker	941:959	a late stage marker	941:959	We have demonstrated that treating peripheral nerve tissue with camptothecin induced both an early marker of apoptosis, cleaved caspase-3 expression, as well as a late stage marker, TUNEL+ DNA fragmentation.
29981947	6	77	theme	DNA	969:971	arg1	fragmentation					973:985	TUNEL+ DNA fragmentation	962:985	TUNEL+ DNA fragmentation	962:985	We have demonstrated that treating peripheral nerve tissue with camptothecin induced both an early marker of apoptosis, cleaved caspase-3 expression, as well as a late stage marker, TUNEL+ DNA fragmentation.
29981947	4	78	theme	easier	641:646	arg1	clearance					648:656	easier clearance	641:656	easier clearance	641:656	During apoptosis, a cell detaches from the extracellular matrix, degrades its internal components, and fragments its contents for easier clearance.
29981947	5	79	theme	tissue	725:730	arg1	decellularization					732:748	tissue decellularization	725:748	tissue decellularization using only mild wash buffers	725:777	We have developed a method that leverages this process to achieve tissue decellularization using only mild wash buffers.
29981947	15	80	theme	acellular	2475:2483	arg1	scaffolds					2491:2499	The resulting acellular nerve scaffolds	2461:2499	The resulting acellular nerve scaffolds	2461:2499	The resulting acellular nerve scaffolds are nearly identical to unprocessed tissue on a microscopic level and elicit low immune responses comparable to an isograft negative control in a model of subcutaneous implantation.
29981947	15	80	theme	acellular	2475:2483	arg1	identical					2512:2520	identical	2512:2520	identical	2512:2520	The resulting acellular nerve scaffolds are nearly identical to unprocessed tissue on a microscopic level and elicit low immune responses comparable to an isograft negative control in a model of subcutaneous implantation.
29981947	11	81	theme	health	1910:1915	arg1	applications					1917:1928	health applications	1910:1928	health applications	1910:1928	STATEMENT OF SIGNIFICANCE Tissue decellularization has expanded the ability to generate non-immunogenic organ replacements for a broad range of health applications.
29981947	7	82	theme	apoptosis-dependent	1049:1067	arg1	manner					1069:1074	an apoptosis-dependent manner	1046:1074	an apoptosis-dependent manner using a gentle wash in hypertonic phosphate buffered saline followed by DNase treatment	1046:1162	Clearance of the cellular components was then achieved in an apoptosis-dependent manner using a gentle wash in hypertonic phosphate buffered saline followed by DNase treatment.
29981947	15	83	theme	immune	2582:2587	arg1	responses					2589:2597	low immune responses	2578:2597	low immune responses comparable to an isograft negative control in a model of subcutaneous implantation	2578:2680	The resulting acellular nerve scaffolds are nearly identical to unprocessed tissue on a microscopic level and elicit low immune responses comparable to an isograft negative control in a model of subcutaneous implantation.
29981947	1	84	theme	tissue	130:135	arg1	structure					137:145	tissue structure	130:145	tissue structure	130:145	Preservation of tissue structure is often a primary goal when optimizing tissue and organ decellularization methods.
29981947	6	85	theme	stage	948:952	arg1	marker					954:959	a late stage marker	941:959	a late stage marker	941:959	We have demonstrated that treating peripheral nerve tissue with camptothecin induced both an early marker of apoptosis, cleaved caspase-3 expression, as well as a late stage marker, TUNEL+ DNA fragmentation.
29981947	6	85	theme	stage	948:952	arg1	fragmentation					973:985	TUNEL+ DNA fragmentation	962:985	TUNEL+ DNA fragmentation	962:985	We have demonstrated that treating peripheral nerve tissue with camptothecin induced both an early marker of apoptosis, cleaved caspase-3 expression, as well as a late stage marker, TUNEL+ DNA fragmentation.
29981947	8	86	theme	cytotoxic	1301:1309	arg1	compound					1311:1318	the cytotoxic compound	1297:1318	the cytotoxic compound based on conditioned media experiments	1297:1357	This wash paradigm did not significantly affect collagen or glycosaminoglycan content, but it was sufficient to remove any trace of the cytotoxic compound based on conditioned media experiments.
29981947	14	87	theme	antigen	2407:2413	arg1	clearance					2415:2423	antigen clearance	2407:2423	antigen clearance using only hypertonic wash buffers	2407:2458	The novelty of this process is in the induction of programmed cell death - or apoptosis - via a general cytotoxin, thereby enabling antigen clearance using only hypertonic wash buffers.
29981947	11	88	theme	applications	1917:1928	arg1	range					1901:1905	a broad range	1893:1905	a broad range of health applications	1893:1928	STATEMENT OF SIGNIFICANCE Tissue decellularization has expanded the ability to generate non-immunogenic organ replacements for a broad range of health applications.
29981947	13	89	theme	peripheral	2202:2211	arg1	tissue					2219:2224	peripheral nerve tissue	2202:2224	peripheral nerve tissue	2202:2224	We have developed a method for effectively, yet gently, removing cellular components from peripheral nerve tissue while preserving the native tissue architecture.
29981947	1	90	theme	decellularization	204:220	arg1	methods					222:228	tissue and organ decellularization methods	187:228	tissue and organ decellularization methods	187:228	Preservation of tissue structure is often a primary goal when optimizing tissue and organ decellularization methods.
29981947	8	91	theme	conditioned	1329:1339	arg1	media					1341:1345	conditioned media	1329:1345	conditioned media experiments	1329:1357	This wash paradigm did not significantly affect collagen or glycosaminoglycan content, but it was sufficient to remove any trace of the cytotoxic compound based on conditioned media experiments.
29981947	0	92	theme	tissue	96:101	arg1	structure					103:111	nerve tissue structure	90:111	nerve tissue structure	90:111	Development of an apoptosis-assisted decellularization method for maximal preservation of nerve tissue structure.
29981947	11	93	theme	non-immunogenic	1854:1868	arg1	replacements					1876:1887	non-immunogenic organ replacements	1854:1887	non-immunogenic organ replacements for a broad range of health applications	1854:1928	STATEMENT OF SIGNIFICANCE Tissue decellularization has expanded the ability to generate non-immunogenic organ replacements for a broad range of health applications.
29981947	5	94	theme	mild	761:764	arg1	buffers					771:777	only mild wash buffers	756:777	only mild wash buffers	756:777	We have developed a method that leverages this process to achieve tissue decellularization using only mild wash buffers.
29981947	15	95	theme	negative	2625:2632	arg1	control					2634:2640	an isograft negative control	2613:2640	an isograft negative control in a model of subcutaneous implantation	2613:2680	The resulting acellular nerve scaffolds are nearly identical to unprocessed tissue on a microscopic level and elicit low immune responses comparable to an isograft negative control in a model of subcutaneous implantation.
29981947	13	96	attach	removing	2168:2175	arg2	method					2132:2137	a method	2130:2137	a method for effectively, yet gently, removing cellular components from peripheral nerve tissue while preserving the native tissue architecture	2130:2272	We have developed a method for effectively, yet gently, removing cellular components from peripheral nerve tissue while preserving the native tissue architecture.
29981947	13	96	attach	removing	2168:2175	arg1	tissue					2219:2224	peripheral nerve tissue	2202:2224	peripheral nerve tissue	2202:2224	We have developed a method for effectively, yet gently, removing cellular components from peripheral nerve tissue while preserving the native tissue architecture.
29981947	10	97	dep	ex	1542:1543	arg1	vivo					1545:1548	vivo	1545:1548	vivo	1545:1548	Hence, ex vivo induction of apoptosis is a promising method to decellularize tissue without the use of harsh reagents while better preserving the benefits of native tissue such as tissue-specific composition and microarchitecture.
29981947	12	98	theme	agents	1987:1992	arg1	use					1974:1976	the use	1970:1976	the use of harsh agents for clearing cellular debris, altering the tissue structure and potentially diminishing the pro-regenerative effects	1970:2109	Current technologies typically rely on the use of harsh agents for clearing cellular debris, altering the tissue structure and potentially diminishing the pro-regenerative effects.
29981947	3	99	theme	cellular	413:420	arg1	process					422:428	an innate cellular process	403:428	an innate cellular process	403:428	It may be more beneficial to leverage an innate cellular process such as apoptosis and promote cell removal without the use of damaging reagents.
29981947	0	100	theme	apoptosis-assisted	18:35	arg1	method					55:60	an apoptosis-assisted decellularization method	15:60	an apoptosis-assisted decellularization method for maximal preservation of nerve tissue structure	15:111	Development of an apoptosis-assisted decellularization method for maximal preservation of nerve tissue structure.
29981947	9	101	theme	resulting	1364:1372	arg1	graft					1391:1395	The resulting acellular tissue graft	1360:1395	The resulting acellular tissue graft	1360:1395	The resulting acellular tissue graft was immunogenically tolerated in vivo and exhibited an intact basal lamina microarchitecture mimicking that of native, unprocessed nerve.
29981947	15	102	theme	comparable	2599:2608	arg1	responses					2589:2597	low immune responses	2578:2597	low immune responses comparable to an isograft negative control in a model of subcutaneous implantation	2578:2680	The resulting acellular nerve scaffolds are nearly identical to unprocessed tissue on a microscopic level and elicit low immune responses comparable to an isograft negative control in a model of subcutaneous implantation.
29981947	14	103	theme	death	2342:2346	arg1	induction					2313:2321	the induction	2309:2321	the induction of programmed cell death - or apoptosis - via a general cytotoxin	2309:2387	The novelty of this process is in the induction of programmed cell death - or apoptosis - via a general cytotoxin, thereby enabling antigen clearance using only hypertonic wash buffers.
29981947	0	104	theme	method	55:60	arg1	Development					0:10	Development	0:10	Development of an apoptosis-assisted decellularization method for maximal preservation of nerve tissue structure.	0:112	Development of an apoptosis-assisted decellularization method for maximal preservation of nerve tissue structure.
29981947	9	105	theme	tissue	1384:1389	arg1	graft					1391:1395	The resulting acellular tissue graft	1360:1395	The resulting acellular tissue graft	1360:1395	The resulting acellular tissue graft was immunogenically tolerated in vivo and exhibited an intact basal lamina microarchitecture mimicking that of native, unprocessed nerve.
29981947	6	106	theme	apoptosis	889:897	arg1	marker					954:959	a late stage marker	941:959	a late stage marker	941:959	We have demonstrated that treating peripheral nerve tissue with camptothecin induced both an early marker of apoptosis, cleaved caspase-3 expression, as well as a late stage marker, TUNEL+ DNA fragmentation.
29981947	6	106	theme	apoptosis	889:897	arg1	expression					918:927	cleaved caspase-3 expression	900:927	cleaved caspase-3 expression	900:927	We have demonstrated that treating peripheral nerve tissue with camptothecin induced both an early marker of apoptosis, cleaved caspase-3 expression, as well as a late stage marker, TUNEL+ DNA fragmentation.
29981947	6	106	theme	apoptosis	889:897	arg1	fragmentation					973:985	TUNEL+ DNA fragmentation	962:985	TUNEL+ DNA fragmentation	962:985	We have demonstrated that treating peripheral nerve tissue with camptothecin induced both an early marker of apoptosis, cleaved caspase-3 expression, as well as a late stage marker, TUNEL+ DNA fragmentation.
29981947	6	106	theme	apoptosis	889:897	arg1	marker					879:884	an early marker	870:884	an early marker of apoptosis	870:897	We have demonstrated that treating peripheral nerve tissue with camptothecin induced both an early marker of apoptosis, cleaved caspase-3 expression, as well as a late stage marker, TUNEL+ DNA fragmentation.
29981947	10	107	theme	native	1693:1698	arg1	tissue					1700:1705	native tissue	1693:1705	native tissue	1693:1705	Hence, ex vivo induction of apoptosis is a promising method to decellularize tissue without the use of harsh reagents while better preserving the benefits of native tissue such as tissue-specific composition and microarchitecture.
29981947	10	108	theme	tissue	1700:1705	arg1	microarchitecture					1747:1763	microarchitecture	1747:1763	microarchitecture	1747:1763	Hence, ex vivo induction of apoptosis is a promising method to decellularize tissue without the use of harsh reagents while better preserving the benefits of native tissue such as tissue-specific composition and microarchitecture.
29981947	10	108	theme	tissue	1700:1705	arg1	composition					1731:1741	tissue-specific composition	1715:1741	tissue-specific composition	1715:1741	Hence, ex vivo induction of apoptosis is a promising method to decellularize tissue without the use of harsh reagents while better preserving the benefits of native tissue such as tissue-specific composition and microarchitecture.
29981947	10	108	theme	tissue	1700:1705	arg1	benefits					1681:1688	the benefits	1677:1688	the benefits of native tissue such as tissue-specific composition and microarchitecture	1677:1763	Hence, ex vivo induction of apoptosis is a promising method to decellularize tissue without the use of harsh reagents while better preserving the benefits of native tissue such as tissue-specific composition and microarchitecture.
29981947	0	109	theme	maximal	66:72	arg1	preservation					74:85	maximal preservation	66:85	maximal preservation of nerve tissue structure	66:111	Development of an apoptosis-assisted decellularization method for maximal preservation of nerve tissue structure.
29981947	15	110	from	tissue	2537:2542	arg1	level					2561:2565	a microscopic level	2547:2565	a microscopic level	2547:2565	The resulting acellular nerve scaffolds are nearly identical to unprocessed tissue on a microscopic level and elicit low immune responses comparable to an isograft negative control in a model of subcutaneous implantation.
29981947	4	111	theme	extracellular	554:566	arg1	matrix					568:573	the extracellular matrix	550:573	the extracellular matrix	550:573	During apoptosis, a cell detaches from the extracellular matrix, degrades its internal components, and fragments its contents for easier clearance.
29981947	11	112	theme	SIGNIFICANCE	1779:1790	arg1	decellularization					1799:1815	SIGNIFICANCE Tissue decellularization	1779:1815	SIGNIFICANCE Tissue decellularization	1779:1815	STATEMENT OF SIGNIFICANCE Tissue decellularization has expanded the ability to generate non-immunogenic organ replacements for a broad range of health applications.
29981947	3	113	theme	reagents	501:508	arg1	use					485:487	the use	481:487	the use of damaging reagents	481:508	It may be more beneficial to leverage an innate cellular process such as apoptosis and promote cell removal without the use of damaging reagents.
29981947	15	114	theme	implantation	2669:2680	arg1	model					2647:2651	a model	2645:2651	a model of subcutaneous implantation	2645:2680	The resulting acellular nerve scaffolds are nearly identical to unprocessed tissue on a microscopic level and elicit low immune responses comparable to an isograft negative control in a model of subcutaneous implantation.
29981947	10	115	theme	promising	1578:1586	arg1	induction					1550:1558	ex vivo induction	1542:1558	ex vivo induction of apoptosis	1542:1571	Hence, ex vivo induction of apoptosis is a promising method to decellularize tissue without the use of harsh reagents while better preserving the benefits of native tissue such as tissue-specific composition and microarchitecture.
29981947	10	115	theme	promising	1578:1586	arg1	method					1588:1593	a promising method	1576:1593	a promising method to decellularize tissue without the use of harsh reagents while better preserving the benefits of native tissue such as tissue-specific composition and microarchitecture	1576:1763	Hence, ex vivo induction of apoptosis is a promising method to decellularize tissue without the use of harsh reagents while better preserving the benefits of native tissue such as tissue-specific composition and microarchitecture.
31140604	5	0	from	intake	712:717	arg1	group					837:841	the IMO + S + L group	821:841	the IMO + S + L group (P < 0.01)	821:852	RESULTS The average daily feed intake of lactating sows in the IMO + S group (P < 0.05) and the average litter gain and total milk yield in the IMO + S + L group (P < 0.01) were significantly higher than those of the sows in the other treatment groups.
31140604	5	0	from	intake	712:717	arg1	group					752:756	the IMO + S group	740:756	the IMO + S group (P < 0.05)	740:767	RESULTS The average daily feed intake of lactating sows in the IMO + S group (P < 0.05) and the average litter gain and total milk yield in the IMO + S + L group (P < 0.01) were significantly higher than those of the sows in the other treatment groups.
31140604	5	0	from	intake	712:717	arg1	P < 0.05					759:766	P < 0.05	759:766	P < 0.05	759:766	RESULTS The average daily feed intake of lactating sows in the IMO + S group (P < 0.05) and the average litter gain and total milk yield in the IMO + S + L group (P < 0.01) were significantly higher than those of the sows in the other treatment groups.
31140604	5	0	from	intake	712:717	arg1	P < 0.01					844:851	P < 0.01	844:851	P < 0.01	844:851	RESULTS The average daily feed intake of lactating sows in the IMO + S group (P < 0.05) and the average litter gain and total milk yield in the IMO + S + L group (P < 0.01) were significantly higher than those of the sows in the other treatment groups.
31140604	8	1	theme	growth	1280:1285	arg1	hormone					1287:1293	growth hormone	1280:1293	growth hormone	1280:1293	The concentrations of growth hormone and immunoglobulins A and G in milk were higher in sows supplemented with IMO, IMO + S, IMO + L, or IMO + S + L (P < 0.05).
31140604	6	2	theme	alanine	957:963	arg1	aminotransferase					965:980	serum alanine aminotransferase	951:980	serum alanine aminotransferase	951:980	In addition, the serum alanine aminotransferase and alkaline phosphatase concentrations in all treatment groups were lower than those in the CON group (P < 0.05).
31140604	1	3	from	period	94:99	arg1	composition					157:167	milk composition	152:167	milk composition of sows	152:175	supplementation during perinatal period on lactational performance, blood metabolites, and milk composition of sows.
31140604	1	3	from	period	94:99	arg1	performance					116:126	lactational performance	104:126	lactational performance	104:126	supplementation during perinatal period on lactational performance, blood metabolites, and milk composition of sows.
31140604	1	3	from	period	94:99	arg1	metabolites					135:145	blood metabolites	129:145	blood metabolites	129:145	supplementation during perinatal period on lactational performance, blood metabolites, and milk composition of sows.
31140604	2	4	dep	BACKGROUND	178:187	arg1	evaluated					207:215	evaluated	207:215	evaluated the effects of isomaltooligosaccharide (IMO) and Bacillus spp	207:277	BACKGROUND In this study, we evaluated the effects of isomaltooligosaccharide (IMO) and Bacillus spp.
31140604	5	5	from	gain	792:795	arg1	group					837:841	the IMO + S + L group	821:841	the IMO + S + L group (P < 0.01)	821:852	RESULTS The average daily feed intake of lactating sows in the IMO + S group (P < 0.05) and the average litter gain and total milk yield in the IMO + S + L group (P < 0.01) were significantly higher than those of the sows in the other treatment groups.
31140604	5	5	from	gain	792:795	arg1	group					752:756	the IMO + S group	740:756	the IMO + S group (P < 0.05)	740:767	RESULTS The average daily feed intake of lactating sows in the IMO + S group (P < 0.05) and the average litter gain and total milk yield in the IMO + S + L group (P < 0.01) were significantly higher than those of the sows in the other treatment groups.
31140604	5	5	from	gain	792:795	arg1	P < 0.05					759:766	P < 0.05	759:766	P < 0.05	759:766	RESULTS The average daily feed intake of lactating sows in the IMO + S group (P < 0.05) and the average litter gain and total milk yield in the IMO + S + L group (P < 0.01) were significantly higher than those of the sows in the other treatment groups.
31140604	5	5	from	gain	792:795	arg1	P < 0.01					844:851	P < 0.01	844:851	P < 0.01	844:851	RESULTS The average daily feed intake of lactating sows in the IMO + S group (P < 0.05) and the average litter gain and total milk yield in the IMO + S + L group (P < 0.01) were significantly higher than those of the sows in the other treatment groups.
31140604	1	6	theme	sows	172:175	arg1	composition					157:167	milk composition	152:167	milk composition of sows	152:175	supplementation during perinatal period on lactational performance, blood metabolites, and milk composition of sows.
31140604	1	6	theme	sows	172:175	arg1	performance					116:126	lactational performance	104:126	lactational performance	104:126	supplementation during perinatal period on lactational performance, blood metabolites, and milk composition of sows.
31140604	1	6	theme	sows	172:175	arg1	metabolites					135:145	blood metabolites	129:145	blood metabolites	129:145	supplementation during perinatal period on lactational performance, blood metabolites, and milk composition of sows.
31140604	11	7	theme	IMO + S + L	1611:1621	arg1	groups					1633:1638	the IMO + S and IMO + S + L treatment groups	1595:1638	groups	1633:1638	Sows in the IMO + S and IMO + S + L treatment groups exhibited the best performance.
31140604	3	8	theme	milk	350:353	arg1	composition					355:365	milk composition	350:365	milk composition of sows	350:373	in perinatal diets on lactational performance, blood metabolites, and milk composition of sows.
31140604	4	9	theme	CON	490:492	arg1	group					494:498	CON group	490:498	CON group	490:498	Multiparous gestating sows (N = 130) with similar body conditions were fed diets without containing IMO (control, CON group), IMO (IMO group), IMO and Bacillus subtilis (IMO + S group), IMO and Bacillus licheniformis (IMO + L group), and IMO and both B. subtilis and B. licheniformis (IMO + S + L group).
31140604	4	10	theme	gestating	388:396	arg1	sows					398:401	Multiparous gestating sows	376:401	Multiparous gestating sows (N = 130) with similar body conditions	376:440	Multiparous gestating sows (N = 130) with similar body conditions were fed diets without containing IMO (control, CON group), IMO (IMO group), IMO and Bacillus subtilis (IMO + S group), IMO and Bacillus licheniformis (IMO + L group), and IMO and both B. subtilis and B. licheniformis (IMO + S + L group).
31140604	4	10	theme	gestating	388:396	arg1	N = 130					404:410	N = 130	404:410	N = 130	404:410	Multiparous gestating sows (N = 130) with similar body conditions were fed diets without containing IMO (control, CON group), IMO (IMO group), IMO and Bacillus subtilis (IMO + S group), IMO and Bacillus licheniformis (IMO + L group), and IMO and both B. subtilis and B. licheniformis (IMO + S + L group).
31140604	5	11	dep	gain	792:795	arg1	the					773:775	the	773:775	the	773:775	RESULTS The average daily feed intake of lactating sows in the IMO + S group (P < 0.05) and the average litter gain and total milk yield in the IMO + S + L group (P < 0.01) were significantly higher than those of the sows in the other treatment groups.
31140604	4	12	theme	containing	465:474	arg1	IMO					476:478	containing IMO	465:478	containing IMO (control, CON group)	465:499	Multiparous gestating sows (N = 130) with similar body conditions were fed diets without containing IMO (control, CON group), IMO (IMO group), IMO and Bacillus subtilis (IMO + S group), IMO and Bacillus licheniformis (IMO + L group), and IMO and both B. subtilis and B. licheniformis (IMO + S + L group).
31140604	4	12	theme	containing	465:474	arg1	control					481:487	control	481:487	control	481:487	Multiparous gestating sows (N = 130) with similar body conditions were fed diets without containing IMO (control, CON group), IMO (IMO group), IMO and Bacillus subtilis (IMO + S group), IMO and Bacillus licheniformis (IMO + L group), and IMO and both B. subtilis and B. licheniformis (IMO + S + L group).
31140604	6	13	theme	alkaline	986:993	arg1	phosphatase					995:1005	alkaline phosphatase	986:1005	alkaline phosphatase	986:1005	In addition, the serum alanine aminotransferase and alkaline phosphatase concentrations in all treatment groups were lower than those in the CON group (P < 0.05).
31140604	7	14	from	colostrum	1143:1151	arg1	milk					1206:1209	milk	1206:1209	milk	1206:1209	Furthermore, the highest proportion of fat in colostrum (0.05 < P < 0.1) and lowest proportion of lactose in milk were observed in the IMO + L group (P < 0.05).
31140604	5	15	theme	total	801:805	arg1	RESULTS					681:687	RESULTS	681:687	RESULTS The average daily feed intake of lactating sows in the IMO + S group (P < 0.05) and the average litter gain and total milk yield in the IMO + S + L group (P < 0.01)	681:852	RESULTS The average daily feed intake of lactating sows in the IMO + S group (P < 0.05) and the average litter gain and total milk yield in the IMO + S + L group (P < 0.01) were significantly higher than those of the sows in the other treatment groups.
31140604	5	15	theme	total	801:805	arg1	yield					812:816	total milk yield	801:816	total milk yield	801:816	RESULTS The average daily feed intake of lactating sows in the IMO + S group (P < 0.05) and the average litter gain and total milk yield in the IMO + S + L group (P < 0.01) were significantly higher than those of the sows in the other treatment groups.
31140604	5	16	theme	IMO + S + L	825:835	arg1	group					837:841	the IMO + S + L group	821:841	the IMO + S + L group (P < 0.01)	821:852	RESULTS The average daily feed intake of lactating sows in the IMO + S group (P < 0.05) and the average litter gain and total milk yield in the IMO + S + L group (P < 0.01) were significantly higher than those of the sows in the other treatment groups.
31140604	5	16	theme	IMO + S + L	825:835	arg1	P < 0.01					844:851	P < 0.01	844:851	P < 0.01	844:851	RESULTS The average daily feed intake of lactating sows in the IMO + S group (P < 0.05) and the average litter gain and total milk yield in the IMO + S + L group (P < 0.01) were significantly higher than those of the sows in the other treatment groups.
31140604	7	17	from	proportion	1122:1131	arg1	proportion					1181:1190	lowest proportion	1174:1190	lowest proportion of lactose in milk	1174:1209	Furthermore, the highest proportion of fat in colostrum (0.05 < P < 0.1) and lowest proportion of lactose in milk were observed in the IMO + L group (P < 0.05).
31140604	7	17	from	proportion	1122:1131	arg1	colostrum					1143:1151	colostrum (0.05 < P < 0.1)	1143:1168	colostrum (0.05 < P < 0.1)	1143:1168	Furthermore, the highest proportion of fat in colostrum (0.05 < P < 0.1) and lowest proportion of lactose in milk were observed in the IMO + L group (P < 0.05).
31140604	1	18	theme	perinatal	84:92	arg1	period					94:99	perinatal period	84:99	perinatal period on lactational performance, blood metabolites, and milk composition of sows	84:175	supplementation during perinatal period on lactational performance, blood metabolites, and milk composition of sows.
31140604	5	19	from	yield	812:816	arg1	group					837:841	the IMO + S + L group	821:841	the IMO + S + L group (P < 0.01)	821:852	RESULTS The average daily feed intake of lactating sows in the IMO + S group (P < 0.05) and the average litter gain and total milk yield in the IMO + S + L group (P < 0.01) were significantly higher than those of the sows in the other treatment groups.
31140604	5	19	from	yield	812:816	arg1	group					752:756	the IMO + S group	740:756	the IMO + S group (P < 0.05)	740:767	RESULTS The average daily feed intake of lactating sows in the IMO + S group (P < 0.05) and the average litter gain and total milk yield in the IMO + S + L group (P < 0.01) were significantly higher than those of the sows in the other treatment groups.
31140604	5	19	from	yield	812:816	arg1	P < 0.05					759:766	P < 0.05	759:766	P < 0.05	759:766	RESULTS The average daily feed intake of lactating sows in the IMO + S group (P < 0.05) and the average litter gain and total milk yield in the IMO + S + L group (P < 0.01) were significantly higher than those of the sows in the other treatment groups.
31140604	5	19	from	yield	812:816	arg1	P < 0.01					844:851	P < 0.01	844:851	P < 0.01	844:851	RESULTS The average daily feed intake of lactating sows in the IMO + S group (P < 0.05) and the average litter gain and total milk yield in the IMO + S + L group (P < 0.01) were significantly higher than those of the sows in the other treatment groups.
31140604	10	20	theme	lactational	1515:1525	arg1	performance					1527:1537	the sows' lactational performance	1505:1537	the sows' lactational performance	1505:1537	supplementation during the perinatal period improved the sows' lactational performance by affecting their metabolism and milk quality.
31140604	6	21	theme	serum	951:955	arg1	aminotransferase					965:980	serum alanine aminotransferase	951:980	serum alanine aminotransferase	951:980	In addition, the serum alanine aminotransferase and alkaline phosphatase concentrations in all treatment groups were lower than those in the CON group (P < 0.05).
31140604	7	22	theme	lactose	1195:1201	arg1	proportion					1181:1190	lowest proportion	1174:1190	lowest proportion of lactose in milk	1174:1209	Furthermore, the highest proportion of fat in colostrum (0.05 < P < 0.1) and lowest proportion of lactose in milk were observed in the IMO + L group (P < 0.05).
31140604	7	22	theme	lactose	1195:1201	arg1	colostrum					1143:1151	colostrum (0.05 < P < 0.1)	1143:1168	colostrum (0.05 < P < 0.1)	1143:1168	Furthermore, the highest proportion of fat in colostrum (0.05 < P < 0.1) and lowest proportion of lactose in milk were observed in the IMO + L group (P < 0.05).
31140604	5	23	from	sows	732:735	arg1	group					752:756	the IMO + S group	740:756	the IMO + S group (P < 0.05)	740:767	RESULTS The average daily feed intake of lactating sows in the IMO + S group (P < 0.05) and the average litter gain and total milk yield in the IMO + S + L group (P < 0.01) were significantly higher than those of the sows in the other treatment groups.
31140604	5	23	from	sows	732:735	arg1	P < 0.05					759:766	P < 0.05	759:766	P < 0.05	759:766	RESULTS The average daily feed intake of lactating sows in the IMO + S group (P < 0.05) and the average litter gain and total milk yield in the IMO + S + L group (P < 0.01) were significantly higher than those of the sows in the other treatment groups.
31140604	7	24	theme	fat	1136:1138	arg1	proportion					1122:1131	the highest proportion	1110:1131	the highest proportion of fat in colostrum (0.05 < P < 0.1) and lowest proportion of lactose in milk	1110:1209	Furthermore, the highest proportion of fat in colostrum (0.05 < P < 0.1) and lowest proportion of lactose in milk were observed in the IMO + L group (P < 0.05).
31140604	7	25	theme	lowest	1174:1179	arg1	proportion					1181:1190	lowest proportion	1174:1190	lowest proportion of lactose in milk	1174:1209	Furthermore, the highest proportion of fat in colostrum (0.05 < P < 0.1) and lowest proportion of lactose in milk were observed in the IMO + L group (P < 0.05).
31140604	5	26	theme	lactating	722:730	arg1	sows					732:735	lactating sows	722:735	lactating sows in the IMO + S group (P < 0.05)	722:767	RESULTS The average daily feed intake of lactating sows in the IMO + S group (P < 0.05) and the average litter gain and total milk yield in the IMO + S + L group (P < 0.01) were significantly higher than those of the sows in the other treatment groups.
31140604	4	27	theme	Multiparous	376:386	arg1	sows					398:401	Multiparous gestating sows	376:401	Multiparous gestating sows (N = 130) with similar body conditions	376:440	Multiparous gestating sows (N = 130) with similar body conditions were fed diets without containing IMO (control, CON group), IMO (IMO group), IMO and Bacillus subtilis (IMO + S group), IMO and Bacillus licheniformis (IMO + L group), and IMO and both B. subtilis and B. licheniformis (IMO + S + L group).
31140604	4	27	theme	Multiparous	376:386	arg1	N = 130					404:410	N = 130	404:410	N = 130	404:410	Multiparous gestating sows (N = 130) with similar body conditions were fed diets without containing IMO (control, CON group), IMO (IMO group), IMO and Bacillus subtilis (IMO + S group), IMO and Bacillus licheniformis (IMO + L group), and IMO and both B. subtilis and B. licheniformis (IMO + S + L group).
31140604	1	28	theme	lactational	104:114	arg1	performance					116:126	lactational performance	104:126	lactational performance	104:126	supplementation during perinatal period on lactational performance, blood metabolites, and milk composition of sows.
31140604	4	29	theme	IMO + L	594:600	arg1	IMO					562:564	IMO	562:564	IMO	562:564	Multiparous gestating sows (N = 130) with similar body conditions were fed diets without containing IMO (control, CON group), IMO (IMO group), IMO and Bacillus subtilis (IMO + S group), IMO and Bacillus licheniformis (IMO + L group), and IMO and both B. subtilis and B. licheniformis (IMO + S + L group).
31140604	4	29	theme	IMO + L	594:600	arg1	group					602:606	IMO + L group	594:606	IMO + L group	594:606	Multiparous gestating sows (N = 130) with similar body conditions were fed diets without containing IMO (control, CON group), IMO (IMO group), IMO and Bacillus subtilis (IMO + S group), IMO and Bacillus licheniformis (IMO + L group), and IMO and both B. subtilis and B. licheniformis (IMO + S + L group).
31140604	4	30	with	sows	398:401	arg1	conditions					431:440	similar body conditions	418:440	similar body conditions	418:440	Multiparous gestating sows (N = 130) with similar body conditions were fed diets without containing IMO (control, CON group), IMO (IMO group), IMO and Bacillus subtilis (IMO + S group), IMO and Bacillus licheniformis (IMO + L group), and IMO and both B. subtilis and B. licheniformis (IMO + S + L group).
31140604	4	31	theme	IMO	507:509	arg1	IMO					502:504	IMO	502:504	IMO (IMO group)	502:516	Multiparous gestating sows (N = 130) with similar body conditions were fed diets without containing IMO (control, CON group), IMO (IMO group), IMO and Bacillus subtilis (IMO + S group), IMO and Bacillus licheniformis (IMO + L group), and IMO and both B. subtilis and B. licheniformis (IMO + S + L group).
31140604	4	31	theme	IMO	507:509	arg1	group					511:515	IMO group	507:515	IMO group	507:515	Multiparous gestating sows (N = 130) with similar body conditions were fed diets without containing IMO (control, CON group), IMO (IMO group), IMO and Bacillus subtilis (IMO + S group), IMO and Bacillus licheniformis (IMO + L group), and IMO and both B. subtilis and B. licheniformis (IMO + S + L group).
31140604	5	32	dep	RESULTS	681:687	arg1	gain					792:795	average litter gain	777:795	average litter gain	777:795	RESULTS The average daily feed intake of lactating sows in the IMO + S group (P < 0.05) and the average litter gain and total milk yield in the IMO + S + L group (P < 0.01) were significantly higher than those of the sows in the other treatment groups.
31140604	5	32	dep	RESULTS	681:687	arg1	intake					712:717	The average daily feed intake	689:717	The average daily feed intake of lactating sows in the IMO + S group (P < 0.05)	689:767	RESULTS The average daily feed intake of lactating sows in the IMO + S group (P < 0.05) and the average litter gain and total milk yield in the IMO + S + L group (P < 0.01) were significantly higher than those of the sows in the other treatment groups.
31140604	5	32	dep	RESULTS	681:687	arg1	RESULTS					681:687	RESULTS	681:687	RESULTS The average daily feed intake of lactating sows in the IMO + S group (P < 0.05) and the average litter gain and total milk yield in the IMO + S + L group (P < 0.01)	681:852	RESULTS The average daily feed intake of lactating sows in the IMO + S group (P < 0.05) and the average litter gain and total milk yield in the IMO + S + L group (P < 0.01) were significantly higher than those of the sows in the other treatment groups.
31140604	7	33	theme	highest	1114:1120	arg1	proportion					1122:1131	the highest proportion	1110:1131	the highest proportion of fat in colostrum (0.05 < P < 0.1) and lowest proportion of lactose in milk	1110:1209	Furthermore, the highest proportion of fat in colostrum (0.05 < P < 0.1) and lowest proportion of lactose in milk were observed in the IMO + L group (P < 0.05).
31140604	6	34	theme	treatment	1029:1037	arg1	groups					1039:1044	all treatment groups	1025:1044	all treatment groups	1025:1044	In addition, the serum alanine aminotransferase and alkaline phosphatase concentrations in all treatment groups were lower than those in the CON group (P < 0.05).
31140604	7	35	theme	IMO + L	1232:1238	arg1	P < 0.05					1247:1254	P < 0.05	1247:1254	P < 0.05	1247:1254	Furthermore, the highest proportion of fat in colostrum (0.05 < P < 0.1) and lowest proportion of lactose in milk were observed in the IMO + L group (P < 0.05).
31140604	7	35	theme	IMO + L	1232:1238	arg1	group					1240:1244	the IMO + L group	1228:1244	the IMO + L group (P < 0.05)	1228:1255	Furthermore, the highest proportion of fat in colostrum (0.05 < P < 0.1) and lowest proportion of lactose in milk were observed in the IMO + L group (P < 0.05).
31140604	3	36	theme	sows	370:373	arg1	performance					314:324	lactational performance	302:324	lactational performance	302:324	in perinatal diets on lactational performance, blood metabolites, and milk composition of sows.
31140604	3	36	theme	sows	370:373	arg1	metabolites					333:343	blood metabolites	327:343	blood metabolites	327:343	in perinatal diets on lactational performance, blood metabolites, and milk composition of sows.
31140604	3	36	theme	sows	370:373	arg1	composition					355:365	milk composition	350:365	milk composition of sows	350:373	in perinatal diets on lactational performance, blood metabolites, and milk composition of sows.
31140604	11	37	theme	treatment	1623:1631	arg1	groups					1633:1638	the IMO + S and IMO + S + L treatment groups	1595:1638	groups	1633:1638	Sows in the IMO + S and IMO + S + L treatment groups exhibited the best performance.
31140604	2	38	theme	spp	275:277	arg1	effects					221:227	the effects	217:227	the effects of isomaltooligosaccharide (IMO) and Bacillus spp	217:277	BACKGROUND In this study, we evaluated the effects of isomaltooligosaccharide (IMO) and Bacillus spp.
31140604	4	39	theme	IMO + S + L	661:671	arg1	group					673:677	IMO + S + L group	661:677	IMO + S + L group	661:677	Multiparous gestating sows (N = 130) with similar body conditions were fed diets without containing IMO (control, CON group), IMO (IMO group), IMO and Bacillus subtilis (IMO + S group), IMO and Bacillus licheniformis (IMO + L group), and IMO and both B. subtilis and B. licheniformis (IMO + S + L group).
31140604	4	39	theme	IMO + S + L	661:671	arg1	B. subtilis					627:637	B. subtilis	627:637	B. subtilis	627:637	Multiparous gestating sows (N = 130) with similar body conditions were fed diets without containing IMO (control, CON group), IMO (IMO group), IMO and Bacillus subtilis (IMO + S group), IMO and Bacillus licheniformis (IMO + L group), and IMO and both B. subtilis and B. licheniformis (IMO + S + L group).
31140604	8	40	from	concentrations	1262:1275	arg1	milk					1326:1329	milk	1326:1329	milk	1326:1329	The concentrations of growth hormone and immunoglobulins A and G in milk were higher in sows supplemented with IMO, IMO + S, IMO + L, or IMO + S + L (P < 0.05).
31140604	5	41	theme	IMO + S	744:750	arg1	group					752:756	the IMO + S group	740:756	the IMO + S group (P < 0.05)	740:767	RESULTS The average daily feed intake of lactating sows in the IMO + S group (P < 0.05) and the average litter gain and total milk yield in the IMO + S + L group (P < 0.01) were significantly higher than those of the sows in the other treatment groups.
31140604	5	41	theme	IMO + S	744:750	arg1	P < 0.05					759:766	P < 0.05	759:766	P < 0.05	759:766	RESULTS The average daily feed intake of lactating sows in the IMO + S group (P < 0.05) and the average litter gain and total milk yield in the IMO + S + L group (P < 0.01) were significantly higher than those of the sows in the other treatment groups.
31140604	5	42	theme	average	693:699	arg1	intake					712:717	The average daily feed intake	689:717	The average daily feed intake of lactating sows in the IMO + S group (P < 0.05)	689:767	RESULTS The average daily feed intake of lactating sows in the IMO + S group (P < 0.05) and the average litter gain and total milk yield in the IMO + S + L group (P < 0.01) were significantly higher than those of the sows in the other treatment groups.
31140604	5	42	theme	average	693:699	arg1	RESULTS					681:687	RESULTS	681:687	RESULTS The average daily feed intake of lactating sows in the IMO + S group (P < 0.05) and the average litter gain and total milk yield in the IMO + S + L group (P < 0.01)	681:852	RESULTS The average daily feed intake of lactating sows in the IMO + S group (P < 0.05) and the average litter gain and total milk yield in the IMO + S + L group (P < 0.01) were significantly higher than those of the sows in the other treatment groups.
31140604	7	43	located	observed	1216:1223	arg2	proportion					1122:1131	the highest proportion	1110:1131	the highest proportion of fat in colostrum (0.05 < P < 0.1) and lowest proportion of lactose in milk	1110:1209	Furthermore, the highest proportion of fat in colostrum (0.05 < P < 0.1) and lowest proportion of lactose in milk were observed in the IMO + L group (P < 0.05).
31140604	7	43	located	observed	1216:1223	arg1	P < 0.05					1247:1254	P < 0.05	1247:1254	P < 0.05	1247:1254	Furthermore, the highest proportion of fat in colostrum (0.05 < P < 0.1) and lowest proportion of lactose in milk were observed in the IMO + L group (P < 0.05).
31140604	7	43	located	observed	1216:1223	arg1	group					1240:1244	the IMO + L group	1228:1244	the IMO + L group (P < 0.05)	1228:1255	Furthermore, the highest proportion of fat in colostrum (0.05 < P < 0.1) and lowest proportion of lactose in milk were observed in the IMO + L group (P < 0.05).
31140604	2	44	theme	isomaltooligosaccharide	232:254	arg1	effects					221:227	the effects	217:227	the effects of isomaltooligosaccharide (IMO) and Bacillus spp	217:277	BACKGROUND In this study, we evaluated the effects of isomaltooligosaccharide (IMO) and Bacillus spp.
31140604	5	45	theme	daily	701:705	arg1	intake					712:717	The average daily feed intake	689:717	The average daily feed intake of lactating sows in the IMO + S group (P < 0.05)	689:767	RESULTS The average daily feed intake of lactating sows in the IMO + S group (P < 0.05) and the average litter gain and total milk yield in the IMO + S + L group (P < 0.01) were significantly higher than those of the sows in the other treatment groups.
31140604	5	45	theme	daily	701:705	arg1	RESULTS					681:687	RESULTS	681:687	RESULTS The average daily feed intake of lactating sows in the IMO + S group (P < 0.05) and the average litter gain and total milk yield in the IMO + S + L group (P < 0.01)	681:852	RESULTS The average daily feed intake of lactating sows in the IMO + S group (P < 0.05) and the average litter gain and total milk yield in the IMO + S + L group (P < 0.01) were significantly higher than those of the sows in the other treatment groups.
31140604	12	46	theme	Chemical	1690:1697	arg1	Industry					1699:1706	Chemical Industry	1690:1706	Chemical Industry	1690:1706	© 2019 Society of Chemical Industry.
31140604	5	47	theme	sows	732:735	arg1	gain					792:795	average litter gain	777:795	average litter gain	777:795	RESULTS The average daily feed intake of lactating sows in the IMO + S group (P < 0.05) and the average litter gain and total milk yield in the IMO + S + L group (P < 0.01) were significantly higher than those of the sows in the other treatment groups.
31140604	5	47	theme	sows	732:735	arg1	RESULTS					681:687	RESULTS	681:687	RESULTS The average daily feed intake of lactating sows in the IMO + S group (P < 0.05) and the average litter gain and total milk yield in the IMO + S + L group (P < 0.01)	681:852	RESULTS The average daily feed intake of lactating sows in the IMO + S group (P < 0.05) and the average litter gain and total milk yield in the IMO + S + L group (P < 0.01) were significantly higher than those of the sows in the other treatment groups.
31140604	5	47	theme	sows	732:735	arg1	intake					712:717	The average daily feed intake	689:717	The average daily feed intake of lactating sows in the IMO + S group (P < 0.05)	689:767	RESULTS The average daily feed intake of lactating sows in the IMO + S group (P < 0.05) and the average litter gain and total milk yield in the IMO + S + L group (P < 0.01) were significantly higher than those of the sows in the other treatment groups.
31140604	5	47	theme	sows	732:735	arg1	yield					812:816	total milk yield	801:816	total milk yield	801:816	RESULTS The average daily feed intake of lactating sows in the IMO + S group (P < 0.05) and the average litter gain and total milk yield in the IMO + S + L group (P < 0.01) were significantly higher than those of the sows in the other treatment groups.
31140604	5	48	from	group	752:756	arg1	gain					792:795	average litter gain	777:795	average litter gain	777:795	RESULTS The average daily feed intake of lactating sows in the IMO + S group (P < 0.05) and the average litter gain and total milk yield in the IMO + S + L group (P < 0.01) were significantly higher than those of the sows in the other treatment groups.
31140604	5	48	from	group	752:756	arg1	RESULTS					681:687	RESULTS	681:687	RESULTS The average daily feed intake of lactating sows in the IMO + S group (P < 0.05) and the average litter gain and total milk yield in the IMO + S + L group (P < 0.01)	681:852	RESULTS The average daily feed intake of lactating sows in the IMO + S group (P < 0.05) and the average litter gain and total milk yield in the IMO + S + L group (P < 0.01) were significantly higher than those of the sows in the other treatment groups.
31140604	5	48	from	group	752:756	arg1	intake					712:717	The average daily feed intake	689:717	The average daily feed intake of lactating sows in the IMO + S group (P < 0.05)	689:767	RESULTS The average daily feed intake of lactating sows in the IMO + S group (P < 0.05) and the average litter gain and total milk yield in the IMO + S + L group (P < 0.01) were significantly higher than those of the sows in the other treatment groups.
31140604	5	48	from	group	752:756	arg1	yield					812:816	total milk yield	801:816	total milk yield	801:816	RESULTS The average daily feed intake of lactating sows in the IMO + S group (P < 0.05) and the average litter gain and total milk yield in the IMO + S + L group (P < 0.01) were significantly higher than those of the sows in the other treatment groups.
31140604	5	49	theme	feed	707:710	arg1	intake					712:717	The average daily feed intake	689:717	The average daily feed intake of lactating sows in the IMO + S group (P < 0.05)	689:767	RESULTS The average daily feed intake of lactating sows in the IMO + S group (P < 0.05) and the average litter gain and total milk yield in the IMO + S + L group (P < 0.01) were significantly higher than those of the sows in the other treatment groups.
31140604	5	49	theme	feed	707:710	arg1	RESULTS					681:687	RESULTS	681:687	RESULTS The average daily feed intake of lactating sows in the IMO + S group (P < 0.05) and the average litter gain and total milk yield in the IMO + S + L group (P < 0.01)	681:852	RESULTS The average daily feed intake of lactating sows in the IMO + S group (P < 0.05) and the average litter gain and total milk yield in the IMO + S + L group (P < 0.01) were significantly higher than those of the sows in the other treatment groups.
31140604	5	50	theme	average	777:783	arg1	gain					792:795	average litter gain	777:795	average litter gain	777:795	RESULTS The average daily feed intake of lactating sows in the IMO + S group (P < 0.05) and the average litter gain and total milk yield in the IMO + S + L group (P < 0.01) were significantly higher than those of the sows in the other treatment groups.
31140604	5	50	theme	average	777:783	arg1	RESULTS					681:687	RESULTS	681:687	RESULTS The average daily feed intake of lactating sows in the IMO + S group (P < 0.05) and the average litter gain and total milk yield in the IMO + S + L group (P < 0.01)	681:852	RESULTS The average daily feed intake of lactating sows in the IMO + S group (P < 0.05) and the average litter gain and total milk yield in the IMO + S + L group (P < 0.01) were significantly higher than those of the sows in the other treatment groups.
31140604	4	51	theme	fed	447:449	arg1	diets					451:455	fed diets	447:455	fed diets without containing IMO (control, CON group), IMO (IMO group), IMO and Bacillus subtilis (IMO + S group), IMO and Bacillus licheniformis (IMO + L group), and IMO	447:616	Multiparous gestating sows (N = 130) with similar body conditions were fed diets without containing IMO (control, CON group), IMO (IMO group), IMO and Bacillus subtilis (IMO + S group), IMO and Bacillus licheniformis (IMO + L group), and IMO and both B. subtilis and B. licheniformis (IMO + S + L group).
31140604	11	52	from	Sows	1587:1590	arg1	IMO + S					1599:1605	the IMO + S and IMO + S + L treatment groups	1595:1638	IMO + S	1599:1605	Sows in the IMO + S and IMO + S + L treatment groups exhibited the best performance.
31140604	11	52	from	Sows	1587:1590	arg1	groups					1633:1638	the IMO + S and IMO + S + L treatment groups	1595:1638	groups	1633:1638	Sows in the IMO + S and IMO + S + L treatment groups exhibited the best performance.
31140604	1	53	theme	blood	129:133	arg1	metabolites					135:145	blood metabolites	129:145	blood metabolites	129:145	supplementation during perinatal period on lactational performance, blood metabolites, and milk composition of sows.
31140604	11	54	theme	best	1654:1657	arg1	performance					1659:1669	the best performance	1650:1669	the best performance	1650:1669	Sows in the IMO + S and IMO + S + L treatment groups exhibited the best performance.
31140604	3	55	theme	lactational	302:312	arg1	performance					314:324	lactational performance	302:324	lactational performance	302:324	in perinatal diets on lactational performance, blood metabolites, and milk composition of sows.
31140604	5	56	theme	other	910:914	arg1	groups					926:931	the other treatment groups	906:931	the other treatment groups	906:931	RESULTS The average daily feed intake of lactating sows in the IMO + S group (P < 0.05) and the average litter gain and total milk yield in the IMO + S + L group (P < 0.01) were significantly higher than those of the sows in the other treatment groups.
31140604	5	57	theme	milk	807:810	arg1	RESULTS					681:687	RESULTS	681:687	RESULTS The average daily feed intake of lactating sows in the IMO + S group (P < 0.05) and the average litter gain and total milk yield in the IMO + S + L group (P < 0.01)	681:852	RESULTS The average daily feed intake of lactating sows in the IMO + S group (P < 0.05) and the average litter gain and total milk yield in the IMO + S + L group (P < 0.01) were significantly higher than those of the sows in the other treatment groups.
31140604	5	57	theme	milk	807:810	arg1	yield					812:816	total milk yield	801:816	total milk yield	801:816	RESULTS The average daily feed intake of lactating sows in the IMO + S group (P < 0.05) and the average litter gain and total milk yield in the IMO + S + L group (P < 0.01) were significantly higher than those of the sows in the other treatment groups.
31140604	8	58	theme	hormone	1287:1293	arg1	concentrations					1262:1275	The concentrations	1258:1275	The concentrations of growth hormone and immunoglobulins A and G in milk	1258:1329	The concentrations of growth hormone and immunoglobulins A and G in milk were higher in sows supplemented with IMO, IMO + S, IMO + L, or IMO + S + L (P < 0.05).
31140604	8	58	theme	hormone	1287:1293	arg1	higher					1336:1341	higher	1336:1341	higher	1336:1341	The concentrations of growth hormone and immunoglobulins A and G in milk were higher in sows supplemented with IMO, IMO + S, IMO + L, or IMO + S + L (P < 0.05).
31140604	4	59	dep	control	481:487	arg1	group					494:498	CON group	490:498	CON group	490:498	Multiparous gestating sows (N = 130) with similar body conditions were fed diets without containing IMO (control, CON group), IMO (IMO group), IMO and Bacillus subtilis (IMO + S group), IMO and Bacillus licheniformis (IMO + L group), and IMO and both B. subtilis and B. licheniformis (IMO + S + L group).
31140604	3	60	theme	perinatal	283:291	arg1	diets					293:297	perinatal diets	283:297	perinatal diets on lactational performance, blood metabolites, and milk composition of sows	283:373	in perinatal diets on lactational performance, blood metabolites, and milk composition of sows.
31140604	5	61	theme	treatment	916:924	arg1	groups					926:931	the other treatment groups	906:931	the other treatment groups	906:931	RESULTS The average daily feed intake of lactating sows in the IMO + S group (P < 0.05) and the average litter gain and total milk yield in the IMO + S + L group (P < 0.01) were significantly higher than those of the sows in the other treatment groups.
31140604	6	62	from	concentrations	1007:1020	arg1	groups					1039:1044	all treatment groups	1025:1044	all treatment groups	1025:1044	In addition, the serum alanine aminotransferase and alkaline phosphatase concentrations in all treatment groups were lower than those in the CON group (P < 0.05).
31140604	6	63	theme	phosphatase	995:1005	arg1	concentrations					1007:1020	the serum alanine aminotransferase and alkaline phosphatase concentrations	947:1020	the serum alanine aminotransferase and alkaline phosphatase concentrations in all treatment groups	947:1044	In addition, the serum alanine aminotransferase and alkaline phosphatase concentrations in all treatment groups were lower than those in the CON group (P < 0.05).
31140604	6	63	theme	phosphatase	995:1005	arg1	lower					1051:1055	lower	1051:1055	lower	1051:1055	In addition, the serum alanine aminotransferase and alkaline phosphatase concentrations in all treatment groups were lower than those in the CON group (P < 0.05).
31140604	10	64	theme	milk	1573:1576	arg1	quality					1578:1584	milk quality	1573:1584	milk quality	1573:1584	supplementation during the perinatal period improved the sows' lactational performance by affecting their metabolism and milk quality.
31140604	4	65	theme	IMO + S	546:552	arg1	IMO					519:521	IMO	519:521	IMO	519:521	Multiparous gestating sows (N = 130) with similar body conditions were fed diets without containing IMO (control, CON group), IMO (IMO group), IMO and Bacillus subtilis (IMO + S group), IMO and Bacillus licheniformis (IMO + L group), and IMO and both B. subtilis and B. licheniformis (IMO + S + L group).
31140604	4	65	theme	IMO + S	546:552	arg1	group					554:558	IMO + S group	546:558	IMO + S group	546:558	Multiparous gestating sows (N = 130) with similar body conditions were fed diets without containing IMO (control, CON group), IMO (IMO group), IMO and Bacillus subtilis (IMO + S group), IMO and Bacillus licheniformis (IMO + L group), and IMO and both B. subtilis and B. licheniformis (IMO + S + L group).
31140604	8	66	theme	immunoglobulins A	1299:1315	arg1	concentrations					1262:1275	The concentrations	1258:1275	The concentrations of growth hormone and immunoglobulins A and G in milk	1258:1329	The concentrations of growth hormone and immunoglobulins A and G in milk were higher in sows supplemented with IMO, IMO + S, IMO + L, or IMO + S + L (P < 0.05).
31140604	8	66	theme	immunoglobulins A	1299:1315	arg1	higher					1336:1341	higher	1336:1341	higher	1336:1341	The concentrations of growth hormone and immunoglobulins A and G in milk were higher in sows supplemented with IMO, IMO + S, IMO + L, or IMO + S + L (P < 0.05).
31140604	4	67	theme	body	426:429	arg1	conditions					431:440	similar body conditions	418:440	similar body conditions	418:440	Multiparous gestating sows (N = 130) with similar body conditions were fed diets without containing IMO (control, CON group), IMO (IMO group), IMO and Bacillus subtilis (IMO + S group), IMO and Bacillus licheniformis (IMO + L group), and IMO and both B. subtilis and B. licheniformis (IMO + S + L group).
31140604	6	68	theme	aminotransferase	965:980	arg1	concentrations					1007:1020	the serum alanine aminotransferase and alkaline phosphatase concentrations	947:1020	the serum alanine aminotransferase and alkaline phosphatase concentrations in all treatment groups	947:1044	In addition, the serum alanine aminotransferase and alkaline phosphatase concentrations in all treatment groups were lower than those in the CON group (P < 0.05).
31140604	6	68	theme	aminotransferase	965:980	arg1	lower					1051:1055	lower	1051:1055	lower	1051:1055	In addition, the serum alanine aminotransferase and alkaline phosphatase concentrations in all treatment groups were lower than those in the CON group (P < 0.05).
31140604	3	69	from	diets	293:297	arg1	performance					314:324	lactational performance	302:324	lactational performance	302:324	in perinatal diets on lactational performance, blood metabolites, and milk composition of sows.
31140604	3	69	from	diets	293:297	arg1	metabolites					333:343	blood metabolites	327:343	blood metabolites	327:343	in perinatal diets on lactational performance, blood metabolites, and milk composition of sows.
31140604	3	69	from	diets	293:297	arg1	composition					355:365	milk composition	350:365	milk composition of sows	350:373	in perinatal diets on lactational performance, blood metabolites, and milk composition of sows.
31140604	3	70	theme	blood	327:331	arg1	metabolites					333:343	blood metabolites	327:343	blood metabolites	327:343	in perinatal diets on lactational performance, blood metabolites, and milk composition of sows.
31140604	4	71	theme	similar	418:424	arg1	conditions					431:440	similar body conditions	418:440	similar body conditions	418:440	Multiparous gestating sows (N = 130) with similar body conditions were fed diets without containing IMO (control, CON group), IMO (IMO group), IMO and Bacillus subtilis (IMO + S group), IMO and Bacillus licheniformis (IMO + L group), and IMO and both B. subtilis and B. licheniformis (IMO + S + L group).
31140604	6	72	theme	CON	1075:1077	arg1	group					1079:1083	the CON group	1071:1083	the CON group (P < 0.05)	1071:1094	In addition, the serum alanine aminotransferase and alkaline phosphatase concentrations in all treatment groups were lower than those in the CON group (P < 0.05).
31140604	6	72	theme	CON	1075:1077	arg1	P < 0.05					1086:1093	P < 0.05	1086:1093	P < 0.05	1086:1093	In addition, the serum alanine aminotransferase and alkaline phosphatase concentrations in all treatment groups were lower than those in the CON group (P < 0.05).
31140604	7	73	from	proportion	1181:1190	arg1	milk					1206:1209	milk	1206:1209	milk	1206:1209	Furthermore, the highest proportion of fat in colostrum (0.05 < P < 0.1) and lowest proportion of lactose in milk were observed in the IMO + L group (P < 0.05).
31140604	1	74	theme	milk	152:155	arg1	composition					157:167	milk composition	152:167	milk composition of sows	152:175	supplementation during perinatal period on lactational performance, blood metabolites, and milk composition of sows.
31140604	10	75	theme	perinatal	1479:1487	arg1	period					1489:1494	the perinatal period	1475:1494	the perinatal period	1475:1494	supplementation during the perinatal period improved the sows' lactational performance by affecting their metabolism and milk quality.
31140604	5	76	theme	litter	785:790	arg1	gain					792:795	average litter gain	777:795	average litter gain	777:795	RESULTS The average daily feed intake of lactating sows in the IMO + S group (P < 0.05) and the average litter gain and total milk yield in the IMO + S + L group (P < 0.01) were significantly higher than those of the sows in the other treatment groups.
31140604	5	76	theme	litter	785:790	arg1	RESULTS					681:687	RESULTS	681:687	RESULTS The average daily feed intake of lactating sows in the IMO + S group (P < 0.05) and the average litter gain and total milk yield in the IMO + S + L group (P < 0.01)	681:852	RESULTS The average daily feed intake of lactating sows in the IMO + S group (P < 0.05) and the average litter gain and total milk yield in the IMO + S + L group (P < 0.01) were significantly higher than those of the sows in the other treatment groups.
31232683	4	0	theme	NaCl	421:424	arg1	presence					397:404	the presence	393:404	the presence of 0-4 % (w/v) NaCl	393:424	The strain grew in the presence of 0-4 % (w/v) NaCl, between pH 6-9.4, and over a temperature range of 15-40 °C, with optimum growth at 30 °C.
31232683	5	1	theme	strain	543:548	arg1	TKZ-21T					550:556	strain TKZ-21T	543:556	strain TKZ-21T	543:556	The peptidoglycan type of strain TKZ-21T was A4β, containing l-ornithine as diagnostic diamino acid and d-glutamic acid as the interpeptide bridge.
31232683	9	2	dep	analysis	989:996	arg1	the					953:955	the	953:955	the	953:955	On the basis of 16S rRNA gene sequence analysis, strain TKZ-21T was closely related to Cellulomonas fimi (98.5 % sequence similarity) and Cellulomonas biazotea (98.3 %).
31232683	9	2	dep	analysis	989:996	arg1	basis					957:961	basis	957:961	basis	957:961	On the basis of 16S rRNA gene sequence analysis, strain TKZ-21T was closely related to Cellulomonas fimi (98.5 % sequence similarity) and Cellulomonas biazotea (98.3 %).
31232683	11	3	theme	Cellulomonas	1483:1494	arg1	sp					1505:1506	the name Cellulomonas algicola sp	1474:1506	the name Cellulomonas algicola sp	1474:1506	On the basis of fatty acid and MALDI-TOF MS profile analysis, phylogenetic analyses, genomic analysis, and phenotypic data, it is proposed that the isolate be classified as a representative of a novel species of the genus Cellulomonas, with the name Cellulomonas algicola sp.
31232683	10	4	theme	genome	1124:1129	arg1	value					1140:1144	The genome orthoANI value	1120:1144	The genome orthoANI value between strain TKZ-21T and C. biazotea and C. fimi	1120:1195	The genome orthoANI value between strain TKZ-21T and C. biazotea and C. fimi were 84.7 and 84.2 %, respectively.
31232683	10	4	theme	genome	1124:1129	arg1	%					1216:1216	84.7 and 84.2 %	1202:1216	84.7 and 84.2 %	1202:1216	The genome orthoANI value between strain TKZ-21T and C. biazotea and C. fimi were 84.7 and 84.2 %, respectively.
31232683	5	5	theme	TKZ-21T	550:556	arg1	type					535:538	The peptidoglycan type	517:538	The peptidoglycan type of strain TKZ-21T	517:556	The peptidoglycan type of strain TKZ-21T was A4β, containing l-ornithine as diagnostic diamino acid and d-glutamic acid as the interpeptide bridge.
31232683	5	5	theme	TKZ-21T	550:556	arg1	A4β					562:564	A4β	562:564	A4β	562:564	The peptidoglycan type of strain TKZ-21T was A4β, containing l-ornithine as diagnostic diamino acid and d-glutamic acid as the interpeptide bridge.
31232683	9	6	theme	98.5 	1056:1060	arg1	%					1061:1061	%	1061:1061	%	1061:1061	On the basis of 16S rRNA gene sequence analysis, strain TKZ-21T was closely related to Cellulomonas fimi (98.5 % sequence similarity) and Cellulomonas biazotea (98.3 %).
31232683	11	7	theme	genomic	1318:1324	arg1	analysis					1326:1333	genomic analysis	1318:1333	genomic analysis	1318:1333	On the basis of fatty acid and MALDI-TOF MS profile analysis, phylogenetic analyses, genomic analysis, and phenotypic data, it is proposed that the isolate be classified as a representative of a novel species of the genus Cellulomonas, with the name Cellulomonas algicola sp.
31232683	7	8	theme	ninhydrin-positive	775:792	arg1	glycolipid					794:803	ninhydrin-positive glycolipid	775:803	ninhydrin-positive glycolipid	775:803	The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, ninhydrin-positive glycolipid, and unidentified phospholipids.
31232683	4	9	theme	15-40 °C	477:484	arg1	range					468:472	a temperature range	454:472	a temperature range of 15-40 °C	454:484	The strain grew in the presence of 0-4 % (w/v) NaCl, between pH 6-9.4, and over a temperature range of 15-40 °C, with optimum growth at 30 °C.
31232683	5	10	theme	d-glutamic	621:630	arg1	acid					632:635	d-glutamic acid	621:635	d-glutamic acid	621:635	The peptidoglycan type of strain TKZ-21T was A4β, containing l-ornithine as diagnostic diamino acid and d-glutamic acid as the interpeptide bridge.
31232683	11	11	theme	genus	1449:1453	arg1	Cellulomonas					1455:1466	the genus Cellulomonas	1445:1466	the genus Cellulomonas	1445:1466	On the basis of fatty acid and MALDI-TOF MS profile analysis, phylogenetic analyses, genomic analysis, and phenotypic data, it is proposed that the isolate be classified as a representative of a novel species of the genus Cellulomonas, with the name Cellulomonas algicola sp.
31232683	13	12	theme	type	1518:1521	arg1	TKZ-21T					1533:1539	TKZ-21T	1533:1539	TKZ-21T (=NBRC 112905T=TBRC 8129T)	1533:1566	The type strain is TKZ-21T (=NBRC 112905T=TBRC 8129T).
31232683	13	12	theme	type	1518:1521	arg1	strain					1523:1528	The type strain	1514:1528	The type strain	1514:1528	The type strain is TKZ-21T (=NBRC 112905T=TBRC 8129T).
31232683	9	13	theme	%	1061:1061	arg1	similarity					1072:1081	98.5 % sequence similarity	1056:1081	98.5 % sequence similarity	1056:1081	On the basis of 16S rRNA gene sequence analysis, strain TKZ-21T was closely related to Cellulomonas fimi (98.5 % sequence similarity) and Cellulomonas biazotea (98.3 %).
31232683	9	13	theme	%	1061:1061	arg1	fimi					1050:1053	Cellulomonas fimi	1037:1053	Cellulomonas fimi (98.5 % sequence similarity)	1037:1082	On the basis of 16S rRNA gene sequence analysis, strain TKZ-21T was closely related to Cellulomonas fimi (98.5 % sequence similarity) and Cellulomonas biazotea (98.3 %).
31232683	2	14	theme	polyphasic	244:253	arg1	approach					265:272	a polyphasic taxonomic approach	242:272	a polyphasic taxonomic approach	242:272	An actinomycete strain, TKZ-21T, was isolated from a freshwater alga (Chetophoraceae) collected from the Takizawa River, Yamanashi, Japan, and examined using a polyphasic taxonomic approach.
31232683	11	15	theme	novel	1428:1432	arg1	species					1434:1440	a novel species	1426:1440	a novel species	1426:1440	On the basis of fatty acid and MALDI-TOF MS profile analysis, phylogenetic analyses, genomic analysis, and phenotypic data, it is proposed that the isolate be classified as a representative of a novel species of the genus Cellulomonas, with the name Cellulomonas algicola sp.
31232683	4	16	theme	optimum	492:498	arg1	growth					500:505	optimum growth	492:505	optimum growth at 30 °C	492:514	The strain grew in the presence of 0-4 % (w/v) NaCl, between pH 6-9.4, and over a temperature range of 15-40 °C, with optimum growth at 30 °C.
31232683	11	17	theme	acid	1255:1258	arg1	analysis					1285:1292	fatty acid and MALDI-TOF MS profile analysis	1249:1292	fatty acid and MALDI-TOF MS profile analysis	1249:1292	On the basis of fatty acid and MALDI-TOF MS profile analysis, phylogenetic analyses, genomic analysis, and phenotypic data, it is proposed that the isolate be classified as a representative of a novel species of the genus Cellulomonas, with the name Cellulomonas algicola sp.
31232683	11	18	theme	name	1478:1481	arg1	sp					1505:1506	the name Cellulomonas algicola sp	1474:1506	the name Cellulomonas algicola sp	1474:1506	On the basis of fatty acid and MALDI-TOF MS profile analysis, phylogenetic analyses, genomic analysis, and phenotypic data, it is proposed that the isolate be classified as a representative of a novel species of the genus Cellulomonas, with the name Cellulomonas algicola sp.
31232683	8	19	theme	75.6 mol	939:946	arg1	G+C content					923:933	the DNA G+C content	915:933	the DNA G+C content	915:933	The major cellular fatty acids were anteiso-C15 : 0 and anteiso-C17 : 0, and the DNA G+C content was 75.6 mol%.
31232683	8	19	theme	75.6 mol	939:946	arg1	%					947:947	75.6 mol%	939:947	75.6 mol%	939:947	The major cellular fatty acids were anteiso-C15 : 0 and anteiso-C17 : 0, and the DNA G+C content was 75.6 mol%.
31232683	9	20	theme	sequence	1063:1070	arg1	similarity					1072:1081	98.5 % sequence similarity	1056:1081	98.5 % sequence similarity	1056:1081	On the basis of 16S rRNA gene sequence analysis, strain TKZ-21T was closely related to Cellulomonas fimi (98.5 % sequence similarity) and Cellulomonas biazotea (98.3 %).
31232683	9	20	theme	sequence	1063:1070	arg1	fimi					1050:1053	Cellulomonas fimi	1037:1053	Cellulomonas fimi (98.5 % sequence similarity)	1037:1082	On the basis of 16S rRNA gene sequence analysis, strain TKZ-21T was closely related to Cellulomonas fimi (98.5 % sequence similarity) and Cellulomonas biazotea (98.3 %).
31232683	9	21	theme	16S	966:968	arg1	analysis					989:996	16S rRNA gene sequence analysis	966:996	16S rRNA gene sequence analysis	966:996	On the basis of 16S rRNA gene sequence analysis, strain TKZ-21T was closely related to Cellulomonas fimi (98.5 % sequence similarity) and Cellulomonas biazotea (98.3 %).
31232683	11	22	theme	MS	1274:1275	arg1	analysis					1285:1292	fatty acid and MALDI-TOF MS profile analysis	1249:1292	fatty acid and MALDI-TOF MS profile analysis	1249:1292	On the basis of fatty acid and MALDI-TOF MS profile analysis, phylogenetic analyses, genomic analysis, and phenotypic data, it is proposed that the isolate be classified as a representative of a novel species of the genus Cellulomonas, with the name Cellulomonas algicola sp.
31232683	11	23	theme	MALDI-TOF	1264:1272	arg1	MS					1274:1275	MALDI-TOF MS	1264:1275	MALDI-TOF MS	1264:1275	On the basis of fatty acid and MALDI-TOF MS profile analysis, phylogenetic analyses, genomic analysis, and phenotypic data, it is proposed that the isolate be classified as a representative of a novel species of the genus Cellulomonas, with the name Cellulomonas algicola sp.
31232683	4	24	theme	%	413:413	arg1	NaCl					421:424	0-4 % (w/v) NaCl	409:424	0-4 % (w/v) NaCl	409:424	The strain grew in the presence of 0-4 % (w/v) NaCl, between pH 6-9.4, and over a temperature range of 15-40 °C, with optimum growth at 30 °C.
31232683	8	25	dep	anteiso-C15 	874:885	arg1	 0					907:908	 0	907:908	 0	907:908	The major cellular fatty acids were anteiso-C15 : 0 and anteiso-C17 : 0, and the DNA G+C content was 75.6 mol%.
31232683	8	25	dep	anteiso-C15 	874:885	arg1	anteiso-C17 					894:905	anteiso-C17 	894:905	anteiso-C17 	894:905	The major cellular fatty acids were anteiso-C15 : 0 and anteiso-C17 : 0, and the DNA G+C content was 75.6 mol%.
31232683	8	25	dep	anteiso-C15 	874:885	arg1	 0					887:888	 0	887:888	 0	887:888	The major cellular fatty acids were anteiso-C15 : 0 and anteiso-C17 : 0, and the DNA G+C content was 75.6 mol%.
31232683	5	26	theme	peptidoglycan	521:533	arg1	type					535:538	The peptidoglycan type	517:538	The peptidoglycan type of strain TKZ-21T	517:556	The peptidoglycan type of strain TKZ-21T was A4β, containing l-ornithine as diagnostic diamino acid and d-glutamic acid as the interpeptide bridge.
31232683	5	26	theme	peptidoglycan	521:533	arg1	A4β					562:564	A4β	562:564	A4β	562:564	The peptidoglycan type of strain TKZ-21T was A4β, containing l-ornithine as diagnostic diamino acid and d-glutamic acid as the interpeptide bridge.
31232683	1	27	theme	freshwater	67:76	arg1	alga					78:81	a freshwater alga	65:81	a freshwater alga	65:81	nov., an actinobacterium isolated from a freshwater alga.
31232683	9	28	theme	rRNA	970:973	arg1	analysis					989:996	16S rRNA gene sequence analysis	966:996	16S rRNA gene sequence analysis	966:996	On the basis of 16S rRNA gene sequence analysis, strain TKZ-21T was closely related to Cellulomonas fimi (98.5 % sequence similarity) and Cellulomonas biazotea (98.3 %).
31232683	11	29	theme	profile	1277:1283	arg1	analysis					1285:1292	fatty acid and MALDI-TOF MS profile analysis	1249:1292	fatty acid and MALDI-TOF MS profile analysis	1249:1292	On the basis of fatty acid and MALDI-TOF MS profile analysis, phylogenetic analyses, genomic analysis, and phenotypic data, it is proposed that the isolate be classified as a representative of a novel species of the genus Cellulomonas, with the name Cellulomonas algicola sp.
31232683	0	30	theme	algicola	13:20	arg1	sp					22:23	Cellulomonas algicola sp	0:23	Cellulomonas algicola sp.	0:24	Cellulomonas algicola sp.
31232683	9	31	theme	gene	975:978	arg1	analysis					989:996	16S rRNA gene sequence analysis	966:996	16S rRNA gene sequence analysis	966:996	On the basis of 16S rRNA gene sequence analysis, strain TKZ-21T was closely related to Cellulomonas fimi (98.5 % sequence similarity) and Cellulomonas biazotea (98.3 %).
31232683	0	32	theme	Cellulomonas	0:11	arg1	sp					22:23	Cellulomonas algicola sp	0:23	Cellulomonas algicola sp.	0:24	Cellulomonas algicola sp.
31232683	4	33	theme	temperature	456:466	arg1	range					468:472	a temperature range	454:472	a temperature range of 15-40 °C	454:484	The strain grew in the presence of 0-4 % (w/v) NaCl, between pH 6-9.4, and over a temperature range of 15-40 °C, with optimum growth at 30 °C.
31232683	8	34	theme	DNA	919:921	arg1	G+C content					923:933	the DNA G+C content	915:933	the DNA G+C content	915:933	The major cellular fatty acids were anteiso-C15 : 0 and anteiso-C17 : 0, and the DNA G+C content was 75.6 mol%.
31232683	8	34	theme	DNA	919:921	arg1	%					947:947	75.6 mol%	939:947	75.6 mol%	939:947	The major cellular fatty acids were anteiso-C15 : 0 and anteiso-C17 : 0, and the DNA G+C content was 75.6 mol%.
31232683	9	35	theme	strain	999:1004	arg1	TKZ-21T					1006:1012	strain TKZ-21T	999:1012	strain TKZ-21T	999:1012	On the basis of 16S rRNA gene sequence analysis, strain TKZ-21T was closely related to Cellulomonas fimi (98.5 % sequence similarity) and Cellulomonas biazotea (98.3 %).
31232683	1	36	attach	isolated	51:58	arg2	actinobacterium					35:49	an actinobacterium	32:49	an actinobacterium isolated from a freshwater alga	32:81	nov., an actinobacterium isolated from a freshwater alga.
31232683	1	36	attach	isolated	51:58	arg1	alga					78:81	a freshwater alga	65:81	a freshwater alga	65:81	nov., an actinobacterium isolated from a freshwater alga.
31232683	5	37	theme	diagnostic	593:602	arg1	acid					612:615	diagnostic diamino acid	593:615	diagnostic diamino acid	593:615	The peptidoglycan type of strain TKZ-21T was A4β, containing l-ornithine as diagnostic diamino acid and d-glutamic acid as the interpeptide bridge.
31232683	6	38	theme	predominant	669:679	arg1	MK-9					697:700	MK-9	697:700	MK-9(H4)	697:704	The predominant menaquinone was MK-9(H4).
31232683	6	38	theme	predominant	669:679	arg1	menaquinone					681:691	The predominant menaquinone	665:691	The predominant menaquinone	665:691	The predominant menaquinone was MK-9(H4).
31232683	9	39	theme	analysis	989:996	arg1	related					1026:1032	related	1026:1032	related	1026:1032	On the basis of 16S rRNA gene sequence analysis, strain TKZ-21T was closely related to Cellulomonas fimi (98.5 % sequence similarity) and Cellulomonas biazotea (98.3 %).
31232683	9	40	theme	Cellulomonas	1088:1099	arg1	%					1116:1116	98.3 %	1111:1116	98.3 %	1111:1116	On the basis of 16S rRNA gene sequence analysis, strain TKZ-21T was closely related to Cellulomonas fimi (98.5 % sequence similarity) and Cellulomonas biazotea (98.3 %).
31232683	9	40	theme	Cellulomonas	1088:1099	arg1	biazotea					1101:1108	Cellulomonas biazotea	1088:1108	Cellulomonas biazotea (98.3 %)	1088:1117	On the basis of 16S rRNA gene sequence analysis, strain TKZ-21T was closely related to Cellulomonas fimi (98.5 % sequence similarity) and Cellulomonas biazotea (98.3 %).
31232683	5	41	theme	diamino	604:610	arg1	acid					612:615	diagnostic diamino acid	593:615	diagnostic diamino acid	593:615	The peptidoglycan type of strain TKZ-21T was A4β, containing l-ornithine as diagnostic diamino acid and d-glutamic acid as the interpeptide bridge.
31232683	2	42	theme	taxonomic	255:263	arg1	approach					265:272	a polyphasic taxonomic approach	242:272	a polyphasic taxonomic approach	242:272	An actinomycete strain, TKZ-21T, was isolated from a freshwater alga (Chetophoraceae) collected from the Takizawa River, Yamanashi, Japan, and examined using a polyphasic taxonomic approach.
31232683	10	43	theme	strain	1154:1159	arg1	TKZ-21T					1161:1167	strain TKZ-21T	1154:1167	strain TKZ-21T	1154:1167	The genome orthoANI value between strain TKZ-21T and C. biazotea and C. fimi were 84.7 and 84.2 %, respectively.
31232683	1	44	dep	actinobacterium	35:49	arg1	nov.					26:29	nov.	26:29	nov.	26:29	nov., an actinobacterium isolated from a freshwater alga.
31232683	4	45	dep	%	413:413	arg1	w/v					416:418	w/v	416:418	w/v	416:418	The strain grew in the presence of 0-4 % (w/v) NaCl, between pH 6-9.4, and over a temperature range of 15-40 °C, with optimum growth at 30 °C.
31232683	3	46	dep	positive	297:304	arg1	rod-shaped					362:371	rod-shaped	362:371	rod-shaped	362:371	Cells were Gram-stain positive, aerobic, non-sporulating, motile, and coccoid or short rod-shaped.
31232683	7	47	theme	polar	711:715	arg1	lipids					717:722	The polar lipids	707:722	The polar lipids	707:722	The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, ninhydrin-positive glycolipid, and unidentified phospholipids.
31232683	7	47	theme	polar	711:715	arg1	diphosphatidylglycerol					729:750	diphosphatidylglycerol	729:750	diphosphatidylglycerol	729:750	The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, ninhydrin-positive glycolipid, and unidentified phospholipids.
31232683	10	48	theme	biazotea	1176:1183	arg1	fimi					1192:1195	C. biazotea and C. fimi	1173:1195	C. biazotea and C. fimi	1173:1195	The genome orthoANI value between strain TKZ-21T and C. biazotea and C. fimi were 84.7 and 84.2 %, respectively.
31232683	2	49	theme	actinomycete	87:98	arg1	TKZ-21T					108:114	TKZ-21T	108:114	TKZ-21T	108:114	An actinomycete strain, TKZ-21T, was isolated from a freshwater alga (Chetophoraceae) collected from the Takizawa River, Yamanashi, Japan, and examined using a polyphasic taxonomic approach.
31232683	2	49	theme	actinomycete	87:98	arg1	strain					100:105	An actinomycete strain	84:105	An actinomycete strain	84:105	An actinomycete strain, TKZ-21T, was isolated from a freshwater alga (Chetophoraceae) collected from the Takizawa River, Yamanashi, Japan, and examined using a polyphasic taxonomic approach.
31232683	4	50	theme	0-4 	409:412	arg1	%					413:413	%	413:413	%	413:413	The strain grew in the presence of 0-4 % (w/v) NaCl, between pH 6-9.4, and over a temperature range of 15-40 °C, with optimum growth at 30 °C.
31232683	8	51	theme	cellular	848:855	arg1	anteiso-C15 					874:885	anteiso-C15 	874:885	anteiso-C15 	874:885	The major cellular fatty acids were anteiso-C15 : 0 and anteiso-C17 : 0, and the DNA G+C content was 75.6 mol%.
31232683	8	51	theme	cellular	848:855	arg1	acids					863:867	The major cellular fatty acids	838:867	The major cellular fatty acids	838:867	The major cellular fatty acids were anteiso-C15 : 0 and anteiso-C17 : 0, and the DNA G+C content was 75.6 mol%.
31232683	11	52	theme	fatty	1249:1253	arg1	acid					1255:1258	fatty acid	1249:1258	fatty acid	1249:1258	On the basis of fatty acid and MALDI-TOF MS profile analysis, phylogenetic analyses, genomic analysis, and phenotypic data, it is proposed that the isolate be classified as a representative of a novel species of the genus Cellulomonas, with the name Cellulomonas algicola sp.
31232683	2	53	theme	freshwater	137:146	arg1	Chetophoraceae					154:167	Chetophoraceae	154:167	Chetophoraceae	154:167	An actinomycete strain, TKZ-21T, was isolated from a freshwater alga (Chetophoraceae) collected from the Takizawa River, Yamanashi, Japan, and examined using a polyphasic taxonomic approach.
31232683	2	53	theme	freshwater	137:146	arg1	alga					148:151	a freshwater alga	135:151	a freshwater alga (Chetophoraceae) collected from the Takizawa River, Yamanashi, Japan	135:220	An actinomycete strain, TKZ-21T, was isolated from a freshwater alga (Chetophoraceae) collected from the Takizawa River, Yamanashi, Japan, and examined using a polyphasic taxonomic approach.
31232683	8	54	theme	major	842:846	arg1	anteiso-C15 					874:885	anteiso-C15 	874:885	anteiso-C15 	874:885	The major cellular fatty acids were anteiso-C15 : 0 and anteiso-C17 : 0, and the DNA G+C content was 75.6 mol%.
31232683	8	54	theme	major	842:846	arg1	acids					863:867	The major cellular fatty acids	838:867	The major cellular fatty acids	838:867	The major cellular fatty acids were anteiso-C15 : 0 and anteiso-C17 : 0, and the DNA G+C content was 75.6 mol%.
31232683	13	55	theme	=NBRC	1542:1546	arg1	8129T					1561:1565	=NBRC 112905T=TBRC 8129T	1542:1565	=NBRC 112905T=TBRC 8129T	1542:1565	The type strain is TKZ-21T (=NBRC 112905T=TBRC 8129T).
31232683	13	55	theme	=NBRC	1542:1546	arg1	TKZ-21T					1533:1539	TKZ-21T	1533:1539	TKZ-21T (=NBRC 112905T=TBRC 8129T)	1533:1566	The type strain is TKZ-21T (=NBRC 112905T=TBRC 8129T).
31232683	5	56	contain	containing	567:576	arg1	type					535:538	The peptidoglycan type	517:538	The peptidoglycan type of strain TKZ-21T	517:556	The peptidoglycan type of strain TKZ-21T was A4β, containing l-ornithine as diagnostic diamino acid and d-glutamic acid as the interpeptide bridge.
31232683	5	56	contain	containing	567:576	arg2	bridge					657:662	the interpeptide bridge	640:662	the interpeptide bridge	640:662	The peptidoglycan type of strain TKZ-21T was A4β, containing l-ornithine as diagnostic diamino acid and d-glutamic acid as the interpeptide bridge.
31232683	5	56	contain	containing	567:576	arg2	l-ornithine					578:588	l-ornithine	578:588	l-ornithine	578:588	The peptidoglycan type of strain TKZ-21T was A4β, containing l-ornithine as diagnostic diamino acid and d-glutamic acid as the interpeptide bridge.
31232683	5	56	contain	containing	567:576	arg1	A4β					562:564	A4β	562:564	A4β	562:564	The peptidoglycan type of strain TKZ-21T was A4β, containing l-ornithine as diagnostic diamino acid and d-glutamic acid as the interpeptide bridge.
31232683	11	57	theme	algicola	1496:1503	arg1	sp					1505:1506	the name Cellulomonas algicola sp	1474:1506	the name Cellulomonas algicola sp	1474:1506	On the basis of fatty acid and MALDI-TOF MS profile analysis, phylogenetic analyses, genomic analysis, and phenotypic data, it is proposed that the isolate be classified as a representative of a novel species of the genus Cellulomonas, with the name Cellulomonas algicola sp.
31232683	11	58	dep	analysis	1285:1292	arg1	the					1236:1238	the	1236:1238	the	1236:1238	On the basis of fatty acid and MALDI-TOF MS profile analysis, phylogenetic analyses, genomic analysis, and phenotypic data, it is proposed that the isolate be classified as a representative of a novel species of the genus Cellulomonas, with the name Cellulomonas algicola sp.
31232683	11	58	dep	analysis	1285:1292	arg1	basis					1240:1244	basis	1240:1244	basis	1240:1244	On the basis of fatty acid and MALDI-TOF MS profile analysis, phylogenetic analyses, genomic analysis, and phenotypic data, it is proposed that the isolate be classified as a representative of a novel species of the genus Cellulomonas, with the name Cellulomonas algicola sp.
31232683	8	59	theme	fatty	857:861	arg1	anteiso-C15 					874:885	anteiso-C15 	874:885	anteiso-C15 	874:885	The major cellular fatty acids were anteiso-C15 : 0 and anteiso-C17 : 0, and the DNA G+C content was 75.6 mol%.
31232683	8	59	theme	fatty	857:861	arg1	acids					863:867	The major cellular fatty acids	838:867	The major cellular fatty acids	838:867	The major cellular fatty acids were anteiso-C15 : 0 and anteiso-C17 : 0, and the DNA G+C content was 75.6 mol%.
31232683	9	60	theme	sequence	980:987	arg1	analysis					989:996	16S rRNA gene sequence analysis	966:996	16S rRNA gene sequence analysis	966:996	On the basis of 16S rRNA gene sequence analysis, strain TKZ-21T was closely related to Cellulomonas fimi (98.5 % sequence similarity) and Cellulomonas biazotea (98.3 %).
31232683	11	61	theme	species	1434:1440	arg1	representative					1408:1421	a representative	1406:1421	a representative of a novel species of the genus Cellulomonas, with the name Cellulomonas algicola sp	1406:1506	On the basis of fatty acid and MALDI-TOF MS profile analysis, phylogenetic analyses, genomic analysis, and phenotypic data, it is proposed that the isolate be classified as a representative of a novel species of the genus Cellulomonas, with the name Cellulomonas algicola sp.
31232683	11	61	theme	species	1434:1440	arg1	isolate					1381:1387	the isolate	1377:1387	the isolate	1377:1387	On the basis of fatty acid and MALDI-TOF MS profile analysis, phylogenetic analyses, genomic analysis, and phenotypic data, it is proposed that the isolate be classified as a representative of a novel species of the genus Cellulomonas, with the name Cellulomonas algicola sp.
31232683	2	62	attach	isolated	121:128	arg2	strain					100:105	An actinomycete strain	84:105	An actinomycete strain	84:105	An actinomycete strain, TKZ-21T, was isolated from a freshwater alga (Chetophoraceae) collected from the Takizawa River, Yamanashi, Japan, and examined using a polyphasic taxonomic approach.
31232683	2	62	attach	isolated	121:128	arg1	Chetophoraceae					154:167	Chetophoraceae	154:167	Chetophoraceae	154:167	An actinomycete strain, TKZ-21T, was isolated from a freshwater alga (Chetophoraceae) collected from the Takizawa River, Yamanashi, Japan, and examined using a polyphasic taxonomic approach.
31232683	2	62	attach	isolated	121:128	arg1	alga					148:151	a freshwater alga	135:151	a freshwater alga (Chetophoraceae) collected from the Takizawa River, Yamanashi, Japan	135:220	An actinomycete strain, TKZ-21T, was isolated from a freshwater alga (Chetophoraceae) collected from the Takizawa River, Yamanashi, Japan, and examined using a polyphasic taxonomic approach.
31232683	2	62	attach	isolated	121:128	arg2	TKZ-21T					108:114	TKZ-21T	108:114	TKZ-21T	108:114	An actinomycete strain, TKZ-21T, was isolated from a freshwater alga (Chetophoraceae) collected from the Takizawa River, Yamanashi, Japan, and examined using a polyphasic taxonomic approach.
31232683	7	63	theme	unidentified	810:821	arg1	phospholipids					823:835	unidentified phospholipids	810:835	unidentified phospholipids	810:835	The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, ninhydrin-positive glycolipid, and unidentified phospholipids.
31232683	10	64	theme	C.	1189:1190	arg1	fimi					1192:1195	C. biazotea and C. fimi	1173:1195	C. biazotea and C. fimi	1173:1195	The genome orthoANI value between strain TKZ-21T and C. biazotea and C. fimi were 84.7 and 84.2 %, respectively.
31232683	5	65	theme	interpeptide	644:655	arg1	l-ornithine					578:588	l-ornithine	578:588	l-ornithine	578:588	The peptidoglycan type of strain TKZ-21T was A4β, containing l-ornithine as diagnostic diamino acid and d-glutamic acid as the interpeptide bridge.
31232683	5	65	theme	interpeptide	644:655	arg1	bridge					657:662	the interpeptide bridge	640:662	the interpeptide bridge	640:662	The peptidoglycan type of strain TKZ-21T was A4β, containing l-ornithine as diagnostic diamino acid and d-glutamic acid as the interpeptide bridge.
31232683	11	66	theme	phenotypic	1340:1349	arg1	data					1351:1354	phenotypic data	1340:1354	phenotypic data	1340:1354	On the basis of fatty acid and MALDI-TOF MS profile analysis, phylogenetic analyses, genomic analysis, and phenotypic data, it is proposed that the isolate be classified as a representative of a novel species of the genus Cellulomonas, with the name Cellulomonas algicola sp.
31232683	9	67	theme	Cellulomonas	1037:1048	arg1	similarity					1072:1081	98.5 % sequence similarity	1056:1081	98.5 % sequence similarity	1056:1081	On the basis of 16S rRNA gene sequence analysis, strain TKZ-21T was closely related to Cellulomonas fimi (98.5 % sequence similarity) and Cellulomonas biazotea (98.3 %).
31232683	9	67	theme	Cellulomonas	1037:1048	arg1	fimi					1050:1053	Cellulomonas fimi	1037:1053	Cellulomonas fimi (98.5 % sequence similarity)	1037:1082	On the basis of 16S rRNA gene sequence analysis, strain TKZ-21T was closely related to Cellulomonas fimi (98.5 % sequence similarity) and Cellulomonas biazotea (98.3 %).
31232683	11	68	theme	phylogenetic	1295:1306	arg1	analyses					1308:1315	phylogenetic analyses	1295:1315	phylogenetic analyses	1295:1315	On the basis of fatty acid and MALDI-TOF MS profile analysis, phylogenetic analyses, genomic analysis, and phenotypic data, it is proposed that the isolate be classified as a representative of a novel species of the genus Cellulomonas, with the name Cellulomonas algicola sp.
31232683	11	69	with	species	1434:1440	arg1	sp					1505:1506	the name Cellulomonas algicola sp	1474:1506	the name Cellulomonas algicola sp	1474:1506	On the basis of fatty acid and MALDI-TOF MS profile analysis, phylogenetic analyses, genomic analysis, and phenotypic data, it is proposed that the isolate be classified as a representative of a novel species of the genus Cellulomonas, with the name Cellulomonas algicola sp.
31232683	4	70	from	30 °C	510:514	arg1	growth					500:505	optimum growth	492:505	optimum growth at 30 °C	492:514	The strain grew in the presence of 0-4 % (w/v) NaCl, between pH 6-9.4, and over a temperature range of 15-40 °C, with optimum growth at 30 °C.
31232683	11	71	theme	Cellulomonas	1455:1466	arg1	species					1434:1440	a novel species	1426:1440	a novel species	1426:1440	On the basis of fatty acid and MALDI-TOF MS profile analysis, phylogenetic analyses, genomic analysis, and phenotypic data, it is proposed that the isolate be classified as a representative of a novel species of the genus Cellulomonas, with the name Cellulomonas algicola sp.
31232683	13	72	theme	112905T=TBRC	1548:1559	arg1	8129T					1561:1565	=NBRC 112905T=TBRC 8129T	1542:1565	=NBRC 112905T=TBRC 8129T	1542:1565	The type strain is TKZ-21T (=NBRC 112905T=TBRC 8129T).
31232683	13	72	theme	112905T=TBRC	1548:1559	arg1	TKZ-21T					1533:1539	TKZ-21T	1533:1539	TKZ-21T (=NBRC 112905T=TBRC 8129T)	1533:1566	The type strain is TKZ-21T (=NBRC 112905T=TBRC 8129T).
31232683	10	73	theme	orthoANI	1131:1138	arg1	value					1140:1144	The genome orthoANI value	1120:1144	The genome orthoANI value between strain TKZ-21T and C. biazotea and C. fimi	1120:1195	The genome orthoANI value between strain TKZ-21T and C. biazotea and C. fimi were 84.7 and 84.2 %, respectively.
31232683	10	73	theme	orthoANI	1131:1138	arg1	%					1216:1216	84.7 and 84.2 %	1202:1216	84.7 and 84.2 %	1202:1216	The genome orthoANI value between strain TKZ-21T and C. biazotea and C. fimi were 84.7 and 84.2 %, respectively.
30547859	11	0	theme	Nocardioides	1305:1316	arg1	species					1284:1290	a novel species	1276:1290	a novel species	1276:1290	Based on the polyphasic analyses, the isolate is considered to represent a novel species of the genus Nocardioides, for which the name Nocardioidesgansuensis sp.
30547859	3	1	theme	rRNA	183:186	arg1	similarities					202:213	The highest 16S rRNA gene sequence similarities	167:213	The highest 16S rRNA gene sequence similarities	167:213	The highest 16S rRNA gene sequence similarities were to those of Nocardioides sediminis MSL-01T (97.10 %), Nocardioides aquiterrae GW-9T (97.08 %) and Nocardioides terrigena DS-17T (96.94 %).
30547859	7	2	theme	DNA	838:840	arg1	G+C content					842:852	a DNA G+C content	836:852	a DNA G+C content of 70.9 %	836:862	Its genome size was 4.76 Mb, comprising 4517 predicted genes with a DNA G+C content of 70.9 %.
30547859	4	3	theme	rRNA	438:441	arg1	trees					467:471	16S rRNA gene-based phylogenetic trees	434:471	16S rRNA gene-based phylogenetic trees	434:471	Strain WSJ-1T grouped with N. terrigena DS-17T and N. sediminis MSL-01T in 16S rRNA gene-based phylogenetic trees.
30547859	3	4	theme	gene	188:191	arg1	similarities					202:213	The highest 16S rRNA gene sequence similarities	167:213	The highest 16S rRNA gene sequence similarities	167:213	The highest 16S rRNA gene sequence similarities were to those of Nocardioides sediminis MSL-01T (97.10 %), Nocardioides aquiterrae GW-9T (97.08 %) and Nocardioides terrigena DS-17T (96.94 %).
30547859	5	5	theme	WSJ-1T/N.	535:543	arg1	JCM11813T					556:564	WSJ-1T/N. aquiterrae JCM11813T	535:564	WSJ-1T/N. aquiterrae JCM11813T	535:564	The DNA-DNA relatedness of WSJ-1T/N. sediminis JCM19559T and WSJ-1T/N. aquiterrae JCM11813T were 44.8 and 29.2 %, respectively.
30547859	4	6	from	MSL-01T	423:429	arg1	trees					467:471	16S rRNA gene-based phylogenetic trees	434:471	16S rRNA gene-based phylogenetic trees	434:471	Strain WSJ-1T grouped with N. terrigena DS-17T and N. sediminis MSL-01T in 16S rRNA gene-based phylogenetic trees.
30547859	11	7	theme	Nocardioidesgansuensis	1338:1359	arg1	sp					1361:1362	the name Nocardioidesgansuensis sp	1329:1362	the name Nocardioidesgansuensis sp	1329:1362	Based on the polyphasic analyses, the isolate is considered to represent a novel species of the genus Nocardioides, for which the name Nocardioidesgansuensis sp.
30547859	4	8	theme	16S	434:436	arg1	rRNA					438:441	16S rRNA	434:441	16S rRNA gene-based phylogenetic trees	434:471	Strain WSJ-1T grouped with N. terrigena DS-17T and N. sediminis MSL-01T in 16S rRNA gene-based phylogenetic trees.
30547859	3	9	theme	highest	171:177	arg1	rRNA					183:186	The highest 16S rRNA	167:186	The highest 16S rRNA gene sequence similarities	167:213	The highest 16S rRNA gene sequence similarities were to those of Nocardioides sediminis MSL-01T (97.10 %), Nocardioides aquiterrae GW-9T (97.08 %) and Nocardioides terrigena DS-17T (96.94 %).
30547859	5	10	theme	aquiterrae	545:554	arg1	JCM11813T					556:564	WSJ-1T/N. aquiterrae JCM11813T	535:564	WSJ-1T/N. aquiterrae JCM11813T	535:564	The DNA-DNA relatedness of WSJ-1T/N. sediminis JCM19559T and WSJ-1T/N. aquiterrae JCM11813T were 44.8 and 29.2 %, respectively.
30547859	1	11	theme	geopark	48:54	arg1	soil					56:59	geopark soil	48:59	geopark soil	48:59	nov., isolated from geopark soil.
30547859	3	12	theme	16S	179:181	arg1	rRNA					183:186	The highest 16S rRNA	167:186	The highest 16S rRNA gene sequence similarities	167:213	The highest 16S rRNA gene sequence similarities were to those of Nocardioides sediminis MSL-01T (97.10 %), Nocardioides aquiterrae GW-9T (97.08 %) and Nocardioides terrigena DS-17T (96.94 %).
30547859	5	13	theme	JCM11813T	556:564	arg1	%					585:585	44.8 and 29.2 %	571:585	44.8 and 29.2 %	571:585	The DNA-DNA relatedness of WSJ-1T/N. sediminis JCM19559T and WSJ-1T/N. aquiterrae JCM11813T were 44.8 and 29.2 %, respectively.
30547859	5	13	theme	JCM11813T	556:564	arg1	relatedness					486:496	The DNA-DNA relatedness	474:496	The DNA-DNA relatedness of WSJ-1T/N. sediminis JCM19559T and WSJ-1T/N. aquiterrae JCM11813T	474:564	The DNA-DNA relatedness of WSJ-1T/N. sediminis JCM19559T and WSJ-1T/N. aquiterrae JCM11813T were 44.8 and 29.2 %, respectively.
30547859	7	14	theme	genome	774:779	arg1	size					781:784	Its genome size	770:784	Its genome size	770:784	Its genome size was 4.76 Mb, comprising 4517 predicted genes with a DNA G+C content of 70.9 %.
30547859	9	15	theme	polar	1038:1042	arg1	phosphatidylglycerol					1056:1075	phosphatidylglycerol	1056:1075	phosphatidylglycerol	1056:1075	The polar lipids were phosphatidylglycerol, diphosphatidylglycerol and phosphatidylinositol.
30547859	9	15	theme	polar	1038:1042	arg1	lipids					1044:1049	The polar lipids	1034:1049	The polar lipids	1034:1049	The polar lipids were phosphatidylglycerol, diphosphatidylglycerol and phosphatidylinositol.
30547859	8	16	theme	diamino	989:995	arg1	menaquinone-8					889:901	menaquinone-8	889:901	menaquinone-8(H4)	889:905	Strain WSJ-1T contained menaquinone-8(H4) as the major respiratory quinone and ll-2,6-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
30547859	8	16	theme	diamino	989:995	arg1	acid					997:1000	the diagnostic diamino acid	974:1000	the diagnostic diamino acid in the cell-wall peptidoglycan	974:1031	Strain WSJ-1T contained menaquinone-8(H4) as the major respiratory quinone and ll-2,6-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
30547859	2	17	theme	geopark	125:131	arg1	soil					133:136	geopark soil	125:136	geopark soil	125:136	A novel bacterial strain, designated WSJ-1T, was isolated from geopark soil in Gansu province, PR China.
30547859	8	18	theme	major	914:918	arg1	menaquinone-8					889:901	menaquinone-8	889:901	menaquinone-8(H4)	889:905	Strain WSJ-1T contained menaquinone-8(H4) as the major respiratory quinone and ll-2,6-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
30547859	8	18	theme	major	914:918	arg1	quinone					932:938	the major respiratory quinone	910:938	the major respiratory quinone	910:938	Strain WSJ-1T contained menaquinone-8(H4) as the major respiratory quinone and ll-2,6-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
30547859	10	19	theme	fatty	1146:1150	arg1	iso-C16 					1163:1170	iso-C16 	1163:1170	iso-C16 	1163:1170	The major cellular fatty acids were iso-C16 : 0, C18 : 1ω9c and C17 : 1ω8c.
30547859	10	19	theme	fatty	1146:1150	arg1	acids					1152:1156	The major cellular fatty acids	1127:1156	The major cellular fatty acids	1127:1156	The major cellular fatty acids were iso-C16 : 0, C18 : 1ω9c and C17 : 1ω8c.
30547859	6	20	theme	sequences	653:661	arg1	values					630:635	Average nucleotide identity values	602:635	Average nucleotide identity values of whole genome sequences of WSJ-1T/N. terrigena KCTC19217T and WSJ-1T/N. sediminis KCTC19271T	602:730	Average nucleotide identity values of whole genome sequences of WSJ-1T/N. terrigena KCTC19217T and WSJ-1T/N. sediminis KCTC19271T were 78.83 and 78.83 %, respectively.
30547859	13	21	theme	49117T=CCTCC	1416:1427	arg1	2018027T					1432:1439	=KCTC 49117T=CCTCC AB 2018027T	1410:1439	=KCTC 49117T=CCTCC AB 2018027T	1410:1439	The type strain is WSJ-1T (=KCTC 49117T=CCTCC AB 2018027T).
30547859	13	21	theme	49117T=CCTCC	1416:1427	arg1	WSJ-1T					1402:1407	WSJ-1T	1402:1407	WSJ-1T (=KCTC 49117T=CCTCC AB 2018027T)	1402:1440	The type strain is WSJ-1T (=KCTC 49117T=CCTCC AB 2018027T).
30547859	8	22	theme	diagnostic	978:987	arg1	menaquinone-8					889:901	menaquinone-8	889:901	menaquinone-8(H4)	889:905	Strain WSJ-1T contained menaquinone-8(H4) as the major respiratory quinone and ll-2,6-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
30547859	8	22	theme	diagnostic	978:987	arg1	acid					997:1000	the diagnostic diamino acid	974:1000	the diagnostic diamino acid in the cell-wall peptidoglycan	974:1031	Strain WSJ-1T contained menaquinone-8(H4) as the major respiratory quinone and ll-2,6-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
30547859	4	23	theme	sediminis	413:421	arg1	MSL-01T					423:429	N. sediminis MSL-01T	410:429	N. sediminis MSL-01T	410:429	Strain WSJ-1T grouped with N. terrigena DS-17T and N. sediminis MSL-01T in 16S rRNA gene-based phylogenetic trees.
30547859	11	24	theme	polyphasic	1216:1225	arg1	analyses					1227:1234	the polyphasic analyses	1212:1234	the polyphasic analyses	1212:1234	Based on the polyphasic analyses, the isolate is considered to represent a novel species of the genus Nocardioides, for which the name Nocardioidesgansuensis sp.
30547859	5	25	theme	WSJ-1T/N.	501:509	arg1	JCM19559T					521:529	WSJ-1T/N. sediminis JCM19559T	501:529	WSJ-1T/N. sediminis JCM19559T	501:529	The DNA-DNA relatedness of WSJ-1T/N. sediminis JCM19559T and WSJ-1T/N. aquiterrae JCM11813T were 44.8 and 29.2 %, respectively.
30547859	6	26	theme	genome	646:651	arg1	sequences					653:661	whole genome sequences	640:661	whole genome sequences of WSJ-1T/N. terrigena KCTC19217T and WSJ-1T/N. sediminis KCTC19271T	640:730	Average nucleotide identity values of whole genome sequences of WSJ-1T/N. terrigena KCTC19217T and WSJ-1T/N. sediminis KCTC19271T were 78.83 and 78.83 %, respectively.
30547859	10	27	theme	major	1131:1135	arg1	iso-C16 					1163:1170	iso-C16 	1163:1170	iso-C16 	1163:1170	The major cellular fatty acids were iso-C16 : 0, C18 : 1ω9c and C17 : 1ω8c.
30547859	10	27	theme	major	1131:1135	arg1	acids					1152:1156	The major cellular fatty acids	1127:1156	The major cellular fatty acids	1127:1156	The major cellular fatty acids were iso-C16 : 0, C18 : 1ω9c and C17 : 1ω8c.
30547859	4	28	theme	N.	410:411	arg1	MSL-01T					423:429	N. sediminis MSL-01T	410:429	N. sediminis MSL-01T	410:429	Strain WSJ-1T grouped with N. terrigena DS-17T and N. sediminis MSL-01T in 16S rRNA gene-based phylogenetic trees.
30547859	3	29	theme	sequence	193:200	arg1	similarities					202:213	The highest 16S rRNA gene sequence similarities	167:213	The highest 16S rRNA gene sequence similarities	167:213	The highest 16S rRNA gene sequence similarities were to those of Nocardioides sediminis MSL-01T (97.10 %), Nocardioides aquiterrae GW-9T (97.08 %) and Nocardioides terrigena DS-17T (96.94 %).
30547859	5	30	theme	sediminis	511:519	arg1	JCM19559T					521:529	WSJ-1T/N. sediminis JCM19559T	501:529	WSJ-1T/N. sediminis JCM19559T	501:529	The DNA-DNA relatedness of WSJ-1T/N. sediminis JCM19559T and WSJ-1T/N. aquiterrae JCM11813T were 44.8 and 29.2 %, respectively.
30547859	13	31	theme	=KCTC	1410:1414	arg1	2018027T					1432:1439	=KCTC 49117T=CCTCC AB 2018027T	1410:1439	=KCTC 49117T=CCTCC AB 2018027T	1410:1439	The type strain is WSJ-1T (=KCTC 49117T=CCTCC AB 2018027T).
30547859	13	31	theme	=KCTC	1410:1414	arg1	WSJ-1T					1402:1407	WSJ-1T	1402:1407	WSJ-1T (=KCTC 49117T=CCTCC AB 2018027T)	1402:1440	The type strain is WSJ-1T (=KCTC 49117T=CCTCC AB 2018027T).
30547859	6	32	theme	whole	640:644	arg1	sequences					653:661	whole genome sequences	640:661	whole genome sequences of WSJ-1T/N. terrigena KCTC19217T and WSJ-1T/N. sediminis KCTC19271T	640:730	Average nucleotide identity values of whole genome sequences of WSJ-1T/N. terrigena KCTC19217T and WSJ-1T/N. sediminis KCTC19271T were 78.83 and 78.83 %, respectively.
30547859	11	33	theme	novel	1278:1282	arg1	species					1284:1290	a novel species	1276:1290	a novel species	1276:1290	Based on the polyphasic analyses, the isolate is considered to represent a novel species of the genus Nocardioides, for which the name Nocardioidesgansuensis sp.
30547859	2	34	theme	PR	157:158	arg1	province					147:154	Gansu province	141:154	Gansu province	141:154	A novel bacterial strain, designated WSJ-1T, was isolated from geopark soil in Gansu province, PR China.
30547859	2	34	theme	PR	157:158	arg1	China					160:164	PR China	157:164	PR China	157:164	A novel bacterial strain, designated WSJ-1T, was isolated from geopark soil in Gansu province, PR China.
30547859	5	35	theme	JCM19559T	521:529	arg1	%					585:585	44.8 and 29.2 %	571:585	44.8 and 29.2 %	571:585	The DNA-DNA relatedness of WSJ-1T/N. sediminis JCM19559T and WSJ-1T/N. aquiterrae JCM11813T were 44.8 and 29.2 %, respectively.
30547859	5	35	theme	JCM19559T	521:529	arg1	relatedness					486:496	The DNA-DNA relatedness	474:496	The DNA-DNA relatedness of WSJ-1T/N. sediminis JCM19559T and WSJ-1T/N. aquiterrae JCM11813T	474:564	The DNA-DNA relatedness of WSJ-1T/N. sediminis JCM19559T and WSJ-1T/N. aquiterrae JCM11813T were 44.8 and 29.2 %, respectively.
30547859	4	36	from	DS-17T	399:404	arg1	trees					467:471	16S rRNA gene-based phylogenetic trees	434:471	16S rRNA gene-based phylogenetic trees	434:471	Strain WSJ-1T grouped with N. terrigena DS-17T and N. sediminis MSL-01T in 16S rRNA gene-based phylogenetic trees.
30547859	10	37	theme	cellular	1137:1144	arg1	iso-C16 					1163:1170	iso-C16 	1163:1170	iso-C16 	1163:1170	The major cellular fatty acids were iso-C16 : 0, C18 : 1ω9c and C17 : 1ω8c.
30547859	10	37	theme	cellular	1137:1144	arg1	acids					1152:1156	The major cellular fatty acids	1127:1156	The major cellular fatty acids	1127:1156	The major cellular fatty acids were iso-C16 : 0, C18 : 1ω9c and C17 : 1ω8c.
30547859	8	38	theme	ll-2,6-diaminopimelic	944:964	arg1	acid					966:969	ll-2,6-diaminopimelic acid	944:969	ll-2,6-diaminopimelic acid	944:969	Strain WSJ-1T contained menaquinone-8(H4) as the major respiratory quinone and ll-2,6-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
30547859	6	39	theme	identity	621:628	arg1	values					630:635	Average nucleotide identity values	602:635	Average nucleotide identity values of whole genome sequences of WSJ-1T/N. terrigena KCTC19217T and WSJ-1T/N. sediminis KCTC19271T	602:730	Average nucleotide identity values of whole genome sequences of WSJ-1T/N. terrigena KCTC19217T and WSJ-1T/N. sediminis KCTC19271T were 78.83 and 78.83 %, respectively.
30547859	7	40	theme	predicted	815:823	arg1	genes					825:829	4517 predicted genes	810:829	4517 predicted genes	810:829	Its genome size was 4.76 Mb, comprising 4517 predicted genes with a DNA G+C content of 70.9 %.
30547859	6	41	theme	nucleotide	610:619	arg1	values					630:635	Average nucleotide identity values	602:635	Average nucleotide identity values of whole genome sequences of WSJ-1T/N. terrigena KCTC19217T and WSJ-1T/N. sediminis KCTC19271T	602:730	Average nucleotide identity values of whole genome sequences of WSJ-1T/N. terrigena KCTC19217T and WSJ-1T/N. sediminis KCTC19271T were 78.83 and 78.83 %, respectively.
30547859	11	42	theme	genus	1299:1303	arg1	Nocardioides					1305:1316	the genus Nocardioides	1295:1316	the genus Nocardioides	1295:1316	Based on the polyphasic analyses, the isolate is considered to represent a novel species of the genus Nocardioides, for which the name Nocardioidesgansuensis sp.
30547859	4	43	theme	phylogenetic	454:465	arg1	trees					467:471	16S rRNA gene-based phylogenetic trees	434:471	16S rRNA gene-based phylogenetic trees	434:471	Strain WSJ-1T grouped with N. terrigena DS-17T and N. sediminis MSL-01T in 16S rRNA gene-based phylogenetic trees.
30547859	4	44	theme	terrigena	389:397	arg1	DS-17T					399:404	N. terrigena DS-17T	386:404	N. terrigena DS-17T	386:404	Strain WSJ-1T grouped with N. terrigena DS-17T and N. sediminis MSL-01T in 16S rRNA gene-based phylogenetic trees.
30547859	11	45	theme	name	1333:1336	arg1	sp					1361:1362	the name Nocardioidesgansuensis sp	1329:1362	the name Nocardioidesgansuensis sp	1329:1362	Based on the polyphasic analyses, the isolate is considered to represent a novel species of the genus Nocardioides, for which the name Nocardioidesgansuensis sp.
30547859	8	46	theme	Strain	865:870	arg1	WSJ-1T					872:877	Strain WSJ-1T	865:877	Strain WSJ-1T	865:877	Strain WSJ-1T contained menaquinone-8(H4) as the major respiratory quinone and ll-2,6-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
30547859	8	47	from	acid	997:1000	arg1	peptidoglycan					1019:1031	the cell-wall peptidoglycan	1005:1031	the cell-wall peptidoglycan	1005:1031	Strain WSJ-1T contained menaquinone-8(H4) as the major respiratory quinone and ll-2,6-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
30547859	4	48	theme	gene-based	443:452	arg1	trees					467:471	16S rRNA gene-based phylogenetic trees	434:471	16S rRNA gene-based phylogenetic trees	434:471	Strain WSJ-1T grouped with N. terrigena DS-17T and N. sediminis MSL-01T in 16S rRNA gene-based phylogenetic trees.
30547859	4	49	theme	N.	386:387	arg1	DS-17T					399:404	N. terrigena DS-17T	386:404	N. terrigena DS-17T	386:404	Strain WSJ-1T grouped with N. terrigena DS-17T and N. sediminis MSL-01T in 16S rRNA gene-based phylogenetic trees.
30547859	5	50	theme	DNA-DNA	478:484	arg1	%					585:585	44.8 and 29.2 %	571:585	44.8 and 29.2 %	571:585	The DNA-DNA relatedness of WSJ-1T/N. sediminis JCM19559T and WSJ-1T/N. aquiterrae JCM11813T were 44.8 and 29.2 %, respectively.
30547859	5	50	theme	DNA-DNA	478:484	arg1	relatedness					486:496	The DNA-DNA relatedness	474:496	The DNA-DNA relatedness of WSJ-1T/N. sediminis JCM19559T and WSJ-1T/N. aquiterrae JCM11813T	474:564	The DNA-DNA relatedness of WSJ-1T/N. sediminis JCM19559T and WSJ-1T/N. aquiterrae JCM11813T were 44.8 and 29.2 %, respectively.
30547859	2	51	theme	bacterial	70:78	arg1	strain					80:85	A novel bacterial strain	62:85	A novel bacterial strain	62:85	A novel bacterial strain, designated WSJ-1T, was isolated from geopark soil in Gansu province, PR China.
30547859	10	52	dep	iso-C16 	1163:1170	arg1	C18 					1176:1179	C18 	1176:1179	C18 	1176:1179	The major cellular fatty acids were iso-C16 : 0, C18 : 1ω9c and C17 : 1ω8c.
30547859	2	53	theme	Gansu	141:145	arg1	province					147:154	Gansu province	141:154	Gansu province	141:154	A novel bacterial strain, designated WSJ-1T, was isolated from geopark soil in Gansu province, PR China.
30547859	2	53	theme	Gansu	141:145	arg1	China					160:164	PR China	157:164	PR China	157:164	A novel bacterial strain, designated WSJ-1T, was isolated from geopark soil in Gansu province, PR China.
30547859	6	54	theme	Average	602:608	arg1	values					630:635	Average nucleotide identity values	602:635	Average nucleotide identity values of whole genome sequences of WSJ-1T/N. terrigena KCTC19217T and WSJ-1T/N. sediminis KCTC19271T	602:730	Average nucleotide identity values of whole genome sequences of WSJ-1T/N. terrigena KCTC19217T and WSJ-1T/N. sediminis KCTC19271T were 78.83 and 78.83 %, respectively.
30547859	2	55	theme	novel	64:68	arg1	strain					80:85	A novel bacterial strain	62:85	A novel bacterial strain	62:85	A novel bacterial strain, designated WSJ-1T, was isolated from geopark soil in Gansu province, PR China.
30547859	8	56	theme	respiratory	920:930	arg1	menaquinone-8					889:901	menaquinone-8	889:901	menaquinone-8(H4)	889:905	Strain WSJ-1T contained menaquinone-8(H4) as the major respiratory quinone and ll-2,6-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
30547859	8	56	theme	respiratory	920:930	arg1	quinone					932:938	the major respiratory quinone	910:938	the major respiratory quinone	910:938	Strain WSJ-1T contained menaquinone-8(H4) as the major respiratory quinone and ll-2,6-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
30547859	2	57	attach	isolated	111:118	arg2	strain					80:85	A novel bacterial strain	62:85	A novel bacterial strain	62:85	A novel bacterial strain, designated WSJ-1T, was isolated from geopark soil in Gansu province, PR China.
30547859	2	57	attach	isolated	111:118	arg1	soil					133:136	geopark soil	125:136	geopark soil	125:136	A novel bacterial strain, designated WSJ-1T, was isolated from geopark soil in Gansu province, PR China.
30547859	7	58	theme	%	862:862	arg1	G+C content					842:852	a DNA G+C content	836:852	a DNA G+C content of 70.9 %	836:862	Its genome size was 4.76 Mb, comprising 4517 predicted genes with a DNA G+C content of 70.9 %.
30547859	6	59	theme	KCTC19271T	721:730	arg1	sequences					653:661	whole genome sequences	640:661	whole genome sequences of WSJ-1T/N. terrigena KCTC19217T and WSJ-1T/N. sediminis KCTC19271T	640:730	Average nucleotide identity values of whole genome sequences of WSJ-1T/N. terrigena KCTC19217T and WSJ-1T/N. sediminis KCTC19271T were 78.83 and 78.83 %, respectively.
30547859	13	60	theme	type	1387:1390	arg1	WSJ-1T					1402:1407	WSJ-1T	1402:1407	WSJ-1T (=KCTC 49117T=CCTCC AB 2018027T)	1402:1440	The type strain is WSJ-1T (=KCTC 49117T=CCTCC AB 2018027T).
30547859	13	60	theme	type	1387:1390	arg1	strain					1392:1397	The type strain	1383:1397	The type strain	1383:1397	The type strain is WSJ-1T (=KCTC 49117T=CCTCC AB 2018027T).
30547859	4	61	theme	Strain	359:364	arg1	WSJ-1T					366:371	Strain WSJ-1T	359:371	Strain WSJ-1T	359:371	Strain WSJ-1T grouped with N. terrigena DS-17T and N. sediminis MSL-01T in 16S rRNA gene-based phylogenetic trees.
30547859	6	62	theme	KCTC19217T	686:695	arg1	sequences					653:661	whole genome sequences	640:661	whole genome sequences of WSJ-1T/N. terrigena KCTC19217T and WSJ-1T/N. sediminis KCTC19271T	640:730	Average nucleotide identity values of whole genome sequences of WSJ-1T/N. terrigena KCTC19217T and WSJ-1T/N. sediminis KCTC19271T were 78.83 and 78.83 %, respectively.
30547859	10	63	dep	C18 	1176:1179	arg1	C17 					1191:1194	C17 	1191:1194	C17 	1191:1194	The major cellular fatty acids were iso-C16 : 0, C18 : 1ω9c and C17 : 1ω8c.
30547859	10	63	dep	C18 	1176:1179	arg1	 1ω9c					1181:1185	 1ω9c	1181:1185	 1ω9c	1181:1185	The major cellular fatty acids were iso-C16 : 0, C18 : 1ω9c and C17 : 1ω8c.
30547859	10	63	dep	C18 	1176:1179	arg1	 1ω8c					1196:1200	 1ω8c	1196:1200	 1ω8c	1196:1200	The major cellular fatty acids were iso-C16 : 0, C18 : 1ω9c and C17 : 1ω8c.
30547859	13	64	theme	AB	1429:1430	arg1	2018027T					1432:1439	=KCTC 49117T=CCTCC AB 2018027T	1410:1439	=KCTC 49117T=CCTCC AB 2018027T	1410:1439	The type strain is WSJ-1T (=KCTC 49117T=CCTCC AB 2018027T).
30547859	13	64	theme	AB	1429:1430	arg1	WSJ-1T					1402:1407	WSJ-1T	1402:1407	WSJ-1T (=KCTC 49117T=CCTCC AB 2018027T)	1402:1440	The type strain is WSJ-1T (=KCTC 49117T=CCTCC AB 2018027T).
30547859	8	65	theme	cell-wall	1009:1017	arg1	peptidoglycan					1019:1031	the cell-wall peptidoglycan	1005:1031	the cell-wall peptidoglycan	1005:1031	Strain WSJ-1T contained menaquinone-8(H4) as the major respiratory quinone and ll-2,6-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
30547859	8	66	contain	contained	879:887	arg1	WSJ-1T					872:877	Strain WSJ-1T	865:877	Strain WSJ-1T	865:877	Strain WSJ-1T contained menaquinone-8(H4) as the major respiratory quinone and ll-2,6-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
30547859	8	66	contain	contained	879:887	arg2	quinone					932:938	the major respiratory quinone	910:938	the major respiratory quinone	910:938	Strain WSJ-1T contained menaquinone-8(H4) as the major respiratory quinone and ll-2,6-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
30547859	8	66	contain	contained	879:887	arg2	H4					903:904	H4	903:904	H4	903:904	Strain WSJ-1T contained menaquinone-8(H4) as the major respiratory quinone and ll-2,6-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
30547859	8	66	contain	contained	879:887	arg2	menaquinone-8					889:901	menaquinone-8	889:901	menaquinone-8(H4)	889:905	Strain WSJ-1T contained menaquinone-8(H4) as the major respiratory quinone and ll-2,6-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
30547859	8	66	contain	contained	879:887	arg2	acid					997:1000	the diagnostic diamino acid	974:1000	the diagnostic diamino acid in the cell-wall peptidoglycan	974:1031	Strain WSJ-1T contained menaquinone-8(H4) as the major respiratory quinone and ll-2,6-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
31759022	8	0	located	observed	1489:1496	arg1	surface					1505:1511	the surface	1501:1511	the surface of cellulose/cuttlebone scaffolds	1501:1545	A nice cauliflower-like structures and needle-like dents of the spherical aggregates, which are characteristic to hydroxyapatite precursors, were observed on the surface of cellulose/cuttlebone scaffolds by SEM.
31759022	8	0	located	observed	1489:1496	arg2	dents					1394:1398	needle-like dents	1382:1398	needle-like dents	1382:1398	A nice cauliflower-like structures and needle-like dents of the spherical aggregates, which are characteristic to hydroxyapatite precursors, were observed on the surface of cellulose/cuttlebone scaffolds by SEM.
31759022	8	0	located	observed	1489:1496	arg2	structures					1367:1376	nice cauliflower-like structures	1345:1376	nice cauliflower-like structures	1345:1376	A nice cauliflower-like structures and needle-like dents of the spherical aggregates, which are characteristic to hydroxyapatite precursors, were observed on the surface of cellulose/cuttlebone scaffolds by SEM.
31759022	4	1	link	derived	634:640	arg1	polymer					642:648	naturally derived polymer	624:648	naturally derived polymer	624:648	In this study, three-dimensional (3D) porous scaffolds from naturally derived polymer, namely regenerated cellulose, were prepared using a non-hydrolytic sol-gel and lyophilization techniques.
31759022	11	2	theme	tissue	2067:2072	arg1	engineering					2074:2084	bone tissue engineering	2062:2084	bone tissue engineering	2062:2084	Proliferative and osteoconductive effects on the osteoblast-like MG-63 cells demonstrate the cellulose/cuttlebone scaffolds soaked in 10x SBF as a favourable material for bone tissue engineering.
31759022	1	3	theme	physicochemical	295:309	arg1	characteristics					326:340	favourable structural, physicochemical and functional characteristics	272:340	favourable structural, physicochemical and functional characteristics	272:340	Polymer-based scaffolds have already gained popularity in many biomedical applications due to convenient routes for fabrication and favourable structural, physicochemical and functional characteristics.
31759022	6	4	theme	cuttlebone	1010:1019	arg1	activity					998:1005	Biogenic activity	989:1005	Biogenic activity of cuttlebone	989:1019	Biogenic activity of cuttlebone is explained by its chemical composition, which includes polysaccharide β-chitin and macro-, micro- and trace elements favourable for mineralization.
31759022	9	5	from	re-deposition	1680:1692	arg1	surface					1740:1746	scaffold surface	1731:1746	scaffold surface	1731:1746	10x SBF coating enhanced cell attachment to the scaffolds because SBF elements are known to increase bioactivity by inducing re-deposition of carbonate apatite crystallites on scaffold surface.
31759022	1	6	theme	functional	315:324	arg1	characteristics					326:340	favourable structural, physicochemical and functional characteristics	272:340	favourable structural, physicochemical and functional characteristics	272:340	Polymer-based scaffolds have already gained popularity in many biomedical applications due to convenient routes for fabrication and favourable structural, physicochemical and functional characteristics.
31759022	1	7	theme	biomedical	203:212	arg1	applications					214:225	many biomedical applications	198:225	many biomedical applications due to convenient routes for fabrication and favourable structural, physicochemical and functional characteristics	198:340	Polymer-based scaffolds have already gained popularity in many biomedical applications due to convenient routes for fabrication and favourable structural, physicochemical and functional characteristics.
31759022	5	8	theme	osteoconductive	767:781	arg1	properties					783:792	osteoconductive properties	767:792	osteoconductive properties of the polymeric scaffolds	767:819	To induce osteoconductive properties of the polymeric scaffolds, cuttlebone microparticles were immobilized and the surface coating was achieved via in vitro mineralization using 10-fold concentrated simulated body fluid (10x SBF).
31759022	8	9	theme	needle-like	1382:1392	arg1	dents					1394:1398	needle-like dents	1382:1398	needle-like dents	1382:1398	A nice cauliflower-like structures and needle-like dents of the spherical aggregates, which are characteristic to hydroxyapatite precursors, were observed on the surface of cellulose/cuttlebone scaffolds by SEM.
31759022	10	10	theme	calcium	1763:1769	arg1	depositions					1785:1795	calcium and phosphate depositions	1763:1795	calcium and phosphate depositions	1763:1795	Additionally, calcium and phosphate depositions were clearly observed on the developed scaffolds using von Kossa and Alizarin Red S staining.
31759022	7	11	theme	long-term	1215:1223	arg1	mineralization					1245:1258	long-term (24 weeks) in vitro mineralization	1215:1258	long-term (24 weeks) in vitro mineralization	1215:1258	Parallel the scaffolds were examined during long-term (24 weeks) in vitro mineralization in 1x SBF for the purpose to investigate apatite-forming ability of the scaffolds.
31759022	1	12	theme	due	227:229	arg1	applications					214:225	many biomedical applications	198:225	many biomedical applications due to convenient routes for fabrication and favourable structural, physicochemical and functional characteristics	198:340	Polymer-based scaffolds have already gained popularity in many biomedical applications due to convenient routes for fabrication and favourable structural, physicochemical and functional characteristics.
31759022	6	13	theme	polysaccharide	1078:1091	arg1	β-chitin					1093:1100	polysaccharide β-chitin	1078:1100	polysaccharide β-chitin	1078:1100	Biogenic activity of cuttlebone is explained by its chemical composition, which includes polysaccharide β-chitin and macro-, micro- and trace elements favourable for mineralization.
31759022	4	14	theme	non-hydrolytic	703:716	arg1	sol-gel					718:724	a non-hydrolytic sol-gel	701:724	a non-hydrolytic sol-gel	701:724	In this study, three-dimensional (3D) porous scaffolds from naturally derived polymer, namely regenerated cellulose, were prepared using a non-hydrolytic sol-gel and lyophilization techniques.
31759022	5	15	theme	polymeric	801:809	arg1	scaffolds					811:819	the polymeric scaffolds	797:819	the polymeric scaffolds	797:819	To induce osteoconductive properties of the polymeric scaffolds, cuttlebone microparticles were immobilized and the surface coating was achieved via in vitro mineralization using 10-fold concentrated simulated body fluid (10x SBF).
31759022	2	16	theme	polymeric	352:360	arg1	scaffolds					362:370	polymeric scaffolds	352:370	polymeric scaffolds	352:370	However, polymeric scaffolds lack osteoconductivity and some synthetic polymers carry the risk of inflammatory response caused by degradation by-products.
31759022	5	17	theme	simulated	957:965	arg1	fluid					972:976	simulated body fluid	957:976	10-fold concentrated simulated body fluid (10x SBF)	936:986	To induce osteoconductive properties of the polymeric scaffolds, cuttlebone microparticles were immobilized and the surface coating was achieved via in vitro mineralization using 10-fold concentrated simulated body fluid (10x SBF).
31759022	8	18	theme	spherical	1407:1415	arg1	aggregates					1417:1426	the spherical aggregates	1403:1426	the spherical aggregates	1403:1426	A nice cauliflower-like structures and needle-like dents of the spherical aggregates, which are characteristic to hydroxyapatite precursors, were observed on the surface of cellulose/cuttlebone scaffolds by SEM.
31759022	7	19	dep	long-term	1215:1223	arg1	in					1236:1237	in	1236:1237	in	1236:1237	Parallel the scaffolds were examined during long-term (24 weeks) in vitro mineralization in 1x SBF for the purpose to investigate apatite-forming ability of the scaffolds.
31759022	7	19	dep	long-term	1215:1223	arg1	24 weeks					1226:1233	24 weeks	1226:1233	24 weeks	1226:1233	Parallel the scaffolds were examined during long-term (24 weeks) in vitro mineralization in 1x SBF for the purpose to investigate apatite-forming ability of the scaffolds.
31759022	9	20	theme	SBF	1559:1561	arg1	coating					1563:1569	SBF coating	1559:1569	SBF coating	1559:1569	10x SBF coating enhanced cell attachment to the scaffolds because SBF elements are known to increase bioactivity by inducing re-deposition of carbonate apatite crystallites on scaffold surface.
31759022	8	21	theme	nice	1345:1348	arg1	structures					1367:1376	nice cauliflower-like structures	1345:1376	nice cauliflower-like structures	1345:1376	A nice cauliflower-like structures and needle-like dents of the spherical aggregates, which are characteristic to hydroxyapatite precursors, were observed on the surface of cellulose/cuttlebone scaffolds by SEM.
31759022	10	22	theme	phosphate	1775:1783	arg1	depositions					1785:1795	calcium and phosphate depositions	1763:1795	calcium and phosphate depositions	1763:1795	Additionally, calcium and phosphate depositions were clearly observed on the developed scaffolds using von Kossa and Alizarin Red S staining.
31759022	7	23	theme	Parallel	1171:1178	arg1	scaffolds					1184:1192	the scaffolds	1180:1192	Parallel the scaffolds	1171:1192	Parallel the scaffolds were examined during long-term (24 weeks) in vitro mineralization in 1x SBF for the purpose to investigate apatite-forming ability of the scaffolds.
31759022	1	24	theme	Polymer-based	140:152	arg1	scaffolds					154:162	Polymer-based scaffolds	140:162	Polymer-based scaffolds	140:162	Polymer-based scaffolds have already gained popularity in many biomedical applications due to convenient routes for fabrication and favourable structural, physicochemical and functional characteristics.
31759022	7	25	theme	1x	1263:1264	arg1	SBF					1266:1268	1x SBF	1263:1268	1x SBF for the purpose to investigate apatite-forming ability of the scaffolds	1263:1340	Parallel the scaffolds were examined during long-term (24 weeks) in vitro mineralization in 1x SBF for the purpose to investigate apatite-forming ability of the scaffolds.
31759022	11	26	theme	osteoconductive	1909:1923	arg1	effects					1925:1931	Proliferative and osteoconductive effects	1891:1931	Proliferative and osteoconductive effects on the osteoblast-like MG-63 cells	1891:1966	Proliferative and osteoconductive effects on the osteoblast-like MG-63 cells demonstrate the cellulose/cuttlebone scaffolds soaked in 10x SBF as a favourable material for bone tissue engineering.
31759022	0	27	theme	reinforcement	62:74	arg1	effect					4:9	The effect	0:9	The effect of biomimetic coating and cuttlebone microparticle reinforcement on the osteoconductive properties of cellulose-based scaffolds.	0:138	The effect of biomimetic coating and cuttlebone microparticle reinforcement on the osteoconductive properties of cellulose-based scaffolds.
31759022	4	28	theme	three-dimensional	579:595	arg1	scaffolds					609:617	three-dimensional (3D) porous scaffolds	579:617	three-dimensional (3D) porous scaffolds	579:617	In this study, three-dimensional (3D) porous scaffolds from naturally derived polymer, namely regenerated cellulose, were prepared using a non-hydrolytic sol-gel and lyophilization techniques.
31759022	7	29	theme	scaffolds	1332:1340	arg1	ability					1317:1323	apatite-forming ability	1301:1323	apatite-forming ability of the scaffolds	1301:1340	Parallel the scaffolds were examined during long-term (24 weeks) in vitro mineralization in 1x SBF for the purpose to investigate apatite-forming ability of the scaffolds.
31759022	11	30	theme	osteoblast-like	1940:1954	arg1	cells					1962:1966	the osteoblast-like MG-63 cells	1936:1966	the osteoblast-like MG-63 cells	1936:1966	Proliferative and osteoconductive effects on the osteoblast-like MG-63 cells demonstrate the cellulose/cuttlebone scaffolds soaked in 10x SBF as a favourable material for bone tissue engineering.
31759022	9	31	theme	carbonate	1697:1705	arg1	crystallites					1715:1726	carbonate apatite crystallites	1697:1726	carbonate apatite crystallites	1697:1726	10x SBF coating enhanced cell attachment to the scaffolds because SBF elements are known to increase bioactivity by inducing re-deposition of carbonate apatite crystallites on scaffold surface.
31759022	3	32	theme	tissue	544:549	arg1	engineering					551:561	bone tissue engineering	539:561	bone tissue engineering	539:561	Those facts limit their practical use in bone tissue engineering.
31759022	10	33	theme	developed	1826:1834	arg1	scaffolds					1836:1844	the developed scaffolds	1822:1844	the developed scaffolds using von Kossa and Alizarin Red S staining	1822:1888	Additionally, calcium and phosphate depositions were clearly observed on the developed scaffolds using von Kossa and Alizarin Red S staining.
31759022	0	34	theme	cellulose-based	113:127	arg1	scaffolds					129:137	cellulose-based scaffolds	113:137	cellulose-based scaffolds	113:137	The effect of biomimetic coating and cuttlebone microparticle reinforcement on the osteoconductive properties of cellulose-based scaffolds.
31759022	9	35	theme	crystallites	1715:1726	arg1	re-deposition					1680:1692	re-deposition	1680:1692	re-deposition of carbonate apatite crystallites on scaffold surface	1680:1746	10x SBF coating enhanced cell attachment to the scaffolds because SBF elements are known to increase bioactivity by inducing re-deposition of carbonate apatite crystallites on scaffold surface.
31759022	2	36	theme	response	454:461	arg1	risk					433:436	the risk	429:436	the risk of inflammatory response caused by degradation by-products	429:495	However, polymeric scaffolds lack osteoconductivity and some synthetic polymers carry the risk of inflammatory response caused by degradation by-products.
31759022	9	37	theme	scaffold	1731:1738	arg1	surface					1740:1746	scaffold surface	1731:1746	scaffold surface	1731:1746	10x SBF coating enhanced cell attachment to the scaffolds because SBF elements are known to increase bioactivity by inducing re-deposition of carbonate apatite crystallites on scaffold surface.
31759022	8	38	theme	cellulose/cuttlebone	1516:1535	arg1	scaffolds					1537:1545	cellulose/cuttlebone scaffolds	1516:1545	cellulose/cuttlebone scaffolds	1516:1545	A nice cauliflower-like structures and needle-like dents of the spherical aggregates, which are characteristic to hydroxyapatite precursors, were observed on the surface of cellulose/cuttlebone scaffolds by SEM.
31759022	3	39	theme	bone	539:542	arg1	engineering					551:561	bone tissue engineering	539:561	bone tissue engineering	539:561	Those facts limit their practical use in bone tissue engineering.
31759022	5	40	theme	10-fold	936:942	arg1	fluid					972:976	simulated body fluid	957:976	10-fold concentrated simulated body fluid (10x SBF)	936:986	To induce osteoconductive properties of the polymeric scaffolds, cuttlebone microparticles were immobilized and the surface coating was achieved via in vitro mineralization using 10-fold concentrated simulated body fluid (10x SBF).
31759022	4	41	theme	derived	634:640	arg1	polymer					642:648	naturally derived polymer	624:648	naturally derived polymer	624:648	In this study, three-dimensional (3D) porous scaffolds from naturally derived polymer, namely regenerated cellulose, were prepared using a non-hydrolytic sol-gel and lyophilization techniques.
31759022	9	42	theme	cell	1580:1583	arg1	attachment					1585:1594	cell attachment	1580:1594	cell attachment	1580:1594	10x SBF coating enhanced cell attachment to the scaffolds because SBF elements are known to increase bioactivity by inducing re-deposition of carbonate apatite crystallites on scaffold surface.
31759022	0	43	theme	coating	25:31	arg1	effect					4:9	The effect	0:9	The effect of biomimetic coating and cuttlebone microparticle reinforcement on the osteoconductive properties of cellulose-based scaffolds.	0:138	The effect of biomimetic coating and cuttlebone microparticle reinforcement on the osteoconductive properties of cellulose-based scaffolds.
31759022	10	44	theme	S	1879:1879	arg1	staining					1881:1888	von Kossa and Alizarin Red S staining	1852:1888	staining	1881:1888	Additionally, calcium and phosphate depositions were clearly observed on the developed scaffolds using von Kossa and Alizarin Red S staining.
31759022	5	45	theme	in	906:907	arg1	mineralization					915:928	in vitro mineralization	906:928	in vitro mineralization using 10-fold concentrated simulated body fluid (10x SBF)	906:986	To induce osteoconductive properties of the polymeric scaffolds, cuttlebone microparticles were immobilized and the surface coating was achieved via in vitro mineralization using 10-fold concentrated simulated body fluid (10x SBF).
31759022	4	46	theme	porous	602:607	arg1	scaffolds					609:617	three-dimensional (3D) porous scaffolds	579:617	three-dimensional (3D) porous scaffolds	579:617	In this study, three-dimensional (3D) porous scaffolds from naturally derived polymer, namely regenerated cellulose, were prepared using a non-hydrolytic sol-gel and lyophilization techniques.
31759022	10	47	theme	Alizarin	1866:1873	arg1	staining					1881:1888	von Kossa and Alizarin Red S staining	1852:1888	staining	1881:1888	Additionally, calcium and phosphate depositions were clearly observed on the developed scaffolds using von Kossa and Alizarin Red S staining.
31759022	8	48	theme	cauliflower-like	1350:1365	arg1	structures					1367:1376	nice cauliflower-like structures	1345:1376	nice cauliflower-like structures	1345:1376	A nice cauliflower-like structures and needle-like dents of the spherical aggregates, which are characteristic to hydroxyapatite precursors, were observed on the surface of cellulose/cuttlebone scaffolds by SEM.
31759022	1	49	theme	favourable	272:281	arg1	characteristics					326:340	favourable structural, physicochemical and functional characteristics	272:340	favourable structural, physicochemical and functional characteristics	272:340	Polymer-based scaffolds have already gained popularity in many biomedical applications due to convenient routes for fabrication and favourable structural, physicochemical and functional characteristics.
31759022	7	50	theme	apatite-forming	1301:1315	arg1	ability					1317:1323	apatite-forming ability	1301:1323	apatite-forming ability of the scaffolds	1301:1340	Parallel the scaffolds were examined during long-term (24 weeks) in vitro mineralization in 1x SBF for the purpose to investigate apatite-forming ability of the scaffolds.
31759022	5	51	theme	surface	873:879	arg1	coating					881:887	the surface coating	869:887	the surface coating	869:887	To induce osteoconductive properties of the polymeric scaffolds, cuttlebone microparticles were immobilized and the surface coating was achieved via in vitro mineralization using 10-fold concentrated simulated body fluid (10x SBF).
31759022	11	52	theme	bone	2062:2065	arg1	engineering					2074:2084	bone tissue engineering	2062:2084	bone tissue engineering	2062:2084	Proliferative and osteoconductive effects on the osteoblast-like MG-63 cells demonstrate the cellulose/cuttlebone scaffolds soaked in 10x SBF as a favourable material for bone tissue engineering.
31759022	1	53	theme	many	198:201	arg1	applications					214:225	many biomedical applications	198:225	many biomedical applications due to convenient routes for fabrication and favourable structural, physicochemical and functional characteristics	198:340	Polymer-based scaffolds have already gained popularity in many biomedical applications due to convenient routes for fabrication and favourable structural, physicochemical and functional characteristics.
31759022	0	54	theme	cuttlebone	37:46	arg1	reinforcement					62:74	cuttlebone microparticle reinforcement	37:74	cuttlebone microparticle reinforcement	37:74	The effect of biomimetic coating and cuttlebone microparticle reinforcement on the osteoconductive properties of cellulose-based scaffolds.
31759022	6	55	theme	Biogenic	989:996	arg1	activity					998:1005	Biogenic activity	989:1005	Biogenic activity of cuttlebone	989:1019	Biogenic activity of cuttlebone is explained by its chemical composition, which includes polysaccharide β-chitin and macro-, micro- and trace elements favourable for mineralization.
31759022	5	56	dep	in	906:907	arg1	vitro					909:913	vitro	909:913	vitro	909:913	To induce osteoconductive properties of the polymeric scaffolds, cuttlebone microparticles were immobilized and the surface coating was achieved via in vitro mineralization using 10-fold concentrated simulated body fluid (10x SBF).
31759022	7	57	dep	in	1236:1237	arg1	vitro					1239:1243	vitro	1239:1243	vitro	1239:1243	Parallel the scaffolds were examined during long-term (24 weeks) in vitro mineralization in 1x SBF for the purpose to investigate apatite-forming ability of the scaffolds.
31759022	4	58	theme	lyophilization	730:743	arg1	techniques					745:754	a non-hydrolytic sol-gel and lyophilization techniques	701:754	a non-hydrolytic sol-gel and lyophilization techniques	701:754	In this study, three-dimensional (3D) porous scaffolds from naturally derived polymer, namely regenerated cellulose, were prepared using a non-hydrolytic sol-gel and lyophilization techniques.
31759022	10	59	located	observed	1810:1817	arg2	depositions					1785:1795	calcium and phosphate depositions	1763:1795	calcium and phosphate depositions	1763:1795	Additionally, calcium and phosphate depositions were clearly observed on the developed scaffolds using von Kossa and Alizarin Red S staining.
31759022	10	59	located	observed	1810:1817	arg1	scaffolds					1836:1844	the developed scaffolds	1822:1844	the developed scaffolds using von Kossa and Alizarin Red S staining	1822:1888	Additionally, calcium and phosphate depositions were clearly observed on the developed scaffolds using von Kossa and Alizarin Red S staining.
31759022	5	60	theme	concentrated	944:955	arg1	fluid					972:976	simulated body fluid	957:976	10-fold concentrated simulated body fluid (10x SBF)	936:986	To induce osteoconductive properties of the polymeric scaffolds, cuttlebone microparticles were immobilized and the surface coating was achieved via in vitro mineralization using 10-fold concentrated simulated body fluid (10x SBF).
31759022	10	61	theme	Red	1875:1877	arg1	staining					1881:1888	von Kossa and Alizarin Red S staining	1852:1888	staining	1881:1888	Additionally, calcium and phosphate depositions were clearly observed on the developed scaffolds using von Kossa and Alizarin Red S staining.
31759022	5	62	theme	scaffolds	811:819	arg1	properties					783:792	osteoconductive properties	767:792	osteoconductive properties of the polymeric scaffolds	767:819	To induce osteoconductive properties of the polymeric scaffolds, cuttlebone microparticles were immobilized and the surface coating was achieved via in vitro mineralization using 10-fold concentrated simulated body fluid (10x SBF).
31759022	5	63	theme	body	967:970	arg1	fluid					972:976	simulated body fluid	957:976	10-fold concentrated simulated body fluid (10x SBF)	936:986	To induce osteoconductive properties of the polymeric scaffolds, cuttlebone microparticles were immobilized and the surface coating was achieved via in vitro mineralization using 10-fold concentrated simulated body fluid (10x SBF).
31759022	4	64	theme	sol-gel	718:724	arg1	techniques					745:754	a non-hydrolytic sol-gel and lyophilization techniques	701:754	a non-hydrolytic sol-gel and lyophilization techniques	701:754	In this study, three-dimensional (3D) porous scaffolds from naturally derived polymer, namely regenerated cellulose, were prepared using a non-hydrolytic sol-gel and lyophilization techniques.
31759022	6	65	theme	chemical	1041:1048	arg1	composition					1050:1060	its chemical composition	1037:1060	its chemical composition	1037:1060	Biogenic activity of cuttlebone is explained by its chemical composition, which includes polysaccharide β-chitin and macro-, micro- and trace elements favourable for mineralization.
31759022	11	66	from	effects	1925:1931	arg1	cells					1962:1966	the osteoblast-like MG-63 cells	1936:1966	the osteoblast-like MG-63 cells	1936:1966	Proliferative and osteoconductive effects on the osteoblast-like MG-63 cells demonstrate the cellulose/cuttlebone scaffolds soaked in 10x SBF as a favourable material for bone tissue engineering.
31759022	8	67	theme	aggregates	1417:1426	arg1	structures					1367:1376	nice cauliflower-like structures	1345:1376	nice cauliflower-like structures	1345:1376	A nice cauliflower-like structures and needle-like dents of the spherical aggregates, which are characteristic to hydroxyapatite precursors, were observed on the surface of cellulose/cuttlebone scaffolds by SEM.
31759022	8	67	theme	aggregates	1417:1426	arg1	dents					1394:1398	needle-like dents	1382:1398	needle-like dents	1382:1398	A nice cauliflower-like structures and needle-like dents of the spherical aggregates, which are characteristic to hydroxyapatite precursors, were observed on the surface of cellulose/cuttlebone scaffolds by SEM.
31759022	6	68	theme	favourable	1140:1149	arg1	macro-					1106:1111	macro-	1106:1111	macro-	1106:1111	Biogenic activity of cuttlebone is explained by its chemical composition, which includes polysaccharide β-chitin and macro-, micro- and trace elements favourable for mineralization.
31759022	2	69	dep	lack	372:375	arg1	carry					423:427	carry	423:427	lack osteoconductivity and some synthetic polymers carry the risk of inflammatory response caused by degradation by-products	372:495	However, polymeric scaffolds lack osteoconductivity and some synthetic polymers carry the risk of inflammatory response caused by degradation by-products.
31759022	6	70	theme	trace	1125:1129	arg1	elements					1131:1138	trace elements	1125:1138	trace elements	1125:1138	Biogenic activity of cuttlebone is explained by its chemical composition, which includes polysaccharide β-chitin and macro-, micro- and trace elements favourable for mineralization.
31759022	0	71	theme	osteoconductive	83:97	arg1	properties					99:108	the osteoconductive properties	79:108	the osteoconductive properties of cellulose-based scaffolds	79:137	The effect of biomimetic coating and cuttlebone microparticle reinforcement on the osteoconductive properties of cellulose-based scaffolds.
31759022	0	72	from	effect	4:9	arg1	properties					99:108	the osteoconductive properties	79:108	the osteoconductive properties of cellulose-based scaffolds	79:137	The effect of biomimetic coating and cuttlebone microparticle reinforcement on the osteoconductive properties of cellulose-based scaffolds.
31759022	8	73	theme	hydroxyapatite	1457:1470	arg1	precursors					1472:1481	hydroxyapatite precursors	1457:1481	hydroxyapatite precursors	1457:1481	A nice cauliflower-like structures and needle-like dents of the spherical aggregates, which are characteristic to hydroxyapatite precursors, were observed on the surface of cellulose/cuttlebone scaffolds by SEM.
31759022	5	74	theme	cuttlebone	822:831	arg1	microparticles					833:846	cuttlebone microparticles	822:846	cuttlebone microparticles	822:846	To induce osteoconductive properties of the polymeric scaffolds, cuttlebone microparticles were immobilized and the surface coating was achieved via in vitro mineralization using 10-fold concentrated simulated body fluid (10x SBF).
31759022	11	75	theme	MG-63	1956:1960	arg1	cells					1962:1966	the osteoblast-like MG-63 cells	1936:1966	the osteoblast-like MG-63 cells	1936:1966	Proliferative and osteoconductive effects on the osteoblast-like MG-63 cells demonstrate the cellulose/cuttlebone scaffolds soaked in 10x SBF as a favourable material for bone tissue engineering.
31759022	0	76	theme	scaffolds	129:137	arg1	properties					99:108	the osteoconductive properties	79:108	the osteoconductive properties of cellulose-based scaffolds	79:137	The effect of biomimetic coating and cuttlebone microparticle reinforcement on the osteoconductive properties of cellulose-based scaffolds.
31759022	1	77	theme	convenient	234:243	arg1	routes					245:250	convenient routes	234:250	convenient routes for fabrication and favourable structural, physicochemical and functional characteristics	234:340	Polymer-based scaffolds have already gained popularity in many biomedical applications due to convenient routes for fabrication and favourable structural, physicochemical and functional characteristics.
31759022	9	78	theme	apatite	1707:1713	arg1	crystallites					1715:1726	carbonate apatite crystallites	1697:1726	carbonate apatite crystallites	1697:1726	10x SBF coating enhanced cell attachment to the scaffolds because SBF elements are known to increase bioactivity by inducing re-deposition of carbonate apatite crystallites on scaffold surface.
31759022	3	79	theme	practical	522:530	arg1	use					532:534	their practical use	516:534	their practical use in bone tissue engineering	516:561	Those facts limit their practical use in bone tissue engineering.
31759022	4	80	from	polymer	642:648	arg1	scaffolds					609:617	three-dimensional (3D) porous scaffolds	579:617	three-dimensional (3D) porous scaffolds	579:617	In this study, three-dimensional (3D) porous scaffolds from naturally derived polymer, namely regenerated cellulose, were prepared using a non-hydrolytic sol-gel and lyophilization techniques.
31759022	11	81	theme	cellulose/cuttlebone	1984:2003	arg1	scaffolds					2005:2013	the cellulose/cuttlebone scaffolds	1980:2013	the cellulose/cuttlebone scaffolds soaked in 10x SBF as a favourable material for bone tissue engineering	1980:2084	Proliferative and osteoconductive effects on the osteoblast-like MG-63 cells demonstrate the cellulose/cuttlebone scaffolds soaked in 10x SBF as a favourable material for bone tissue engineering.
31759022	2	82	theme	inflammatory	441:452	arg1	response					454:461	inflammatory response	441:461	inflammatory response caused by degradation by-products	441:495	However, polymeric scaffolds lack osteoconductivity and some synthetic polymers carry the risk of inflammatory response caused by degradation by-products.
31759022	0	83	theme	biomimetic	14:23	arg1	coating					25:31	biomimetic coating	14:31	biomimetic coating	14:31	The effect of biomimetic coating and cuttlebone microparticle reinforcement on the osteoconductive properties of cellulose-based scaffolds.
31759022	8	84	dep	structures	1367:1376	arg1	A					1343:1343	A	1343:1343	A	1343:1343	A nice cauliflower-like structures and needle-like dents of the spherical aggregates, which are characteristic to hydroxyapatite precursors, were observed on the surface of cellulose/cuttlebone scaffolds by SEM.
31759022	10	85	theme	von	1852:1854	arg1	Kossa					1856:1860	von Kossa and Alizarin Red S staining	1852:1888	Kossa	1856:1860	Additionally, calcium and phosphate depositions were clearly observed on the developed scaffolds using von Kossa and Alizarin Red S staining.
31759022	9	86	theme	SBF	1621:1623	arg1	elements					1625:1632	SBF elements	1621:1632	SBF elements	1621:1632	10x SBF coating enhanced cell attachment to the scaffolds because SBF elements are known to increase bioactivity by inducing re-deposition of carbonate apatite crystallites on scaffold surface.
31759022	8	87	theme	scaffolds	1537:1545	arg1	surface					1505:1511	the surface	1501:1511	the surface of cellulose/cuttlebone scaffolds	1501:1545	A nice cauliflower-like structures and needle-like dents of the spherical aggregates, which are characteristic to hydroxyapatite precursors, were observed on the surface of cellulose/cuttlebone scaffolds by SEM.
31759022	3	88	from	use	532:534	arg1	engineering					551:561	bone tissue engineering	539:561	bone tissue engineering	539:561	Those facts limit their practical use in bone tissue engineering.
31759022	2	89	theme	degradation	473:483	arg1	by-products					485:495	degradation by-products	473:495	degradation by-products	473:495	However, polymeric scaffolds lack osteoconductivity and some synthetic polymers carry the risk of inflammatory response caused by degradation by-products.
31759022	0	90	theme	microparticle	48:60	arg1	reinforcement					62:74	cuttlebone microparticle reinforcement	37:74	cuttlebone microparticle reinforcement	37:74	The effect of biomimetic coating and cuttlebone microparticle reinforcement on the osteoconductive properties of cellulose-based scaffolds.
31759022	4	91	dep	three-dimensional	579:595	arg1	3D					598:599	3D	598:599	3D	598:599	In this study, three-dimensional (3D) porous scaffolds from naturally derived polymer, namely regenerated cellulose, were prepared using a non-hydrolytic sol-gel and lyophilization techniques.
31759022	11	92	theme	Proliferative	1891:1903	arg1	effects					1925:1931	Proliferative and osteoconductive effects	1891:1931	Proliferative and osteoconductive effects on the osteoblast-like MG-63 cells	1891:1966	Proliferative and osteoconductive effects on the osteoblast-like MG-63 cells demonstrate the cellulose/cuttlebone scaffolds soaked in 10x SBF as a favourable material for bone tissue engineering.
31759022	2	93	theme	synthetic	404:412	arg1	polymers					414:421	some synthetic polymers	399:421	some synthetic polymers	399:421	However, polymeric scaffolds lack osteoconductivity and some synthetic polymers carry the risk of inflammatory response caused by degradation by-products.
31759022	5	94	dep	fluid	972:976	arg1	SBF					983:985	SBF	983:985	10x SBF	979:985	To induce osteoconductive properties of the polymeric scaffolds, cuttlebone microparticles were immobilized and the surface coating was achieved via in vitro mineralization using 10-fold concentrated simulated body fluid (10x SBF).
31759022	11	95	theme	favourable	2038:2047	arg1	material					2049:2056	a favourable material	2036:2056	a favourable material for bone tissue engineering	2036:2084	Proliferative and osteoconductive effects on the osteoblast-like MG-63 cells demonstrate the cellulose/cuttlebone scaffolds soaked in 10x SBF as a favourable material for bone tissue engineering.
31759022	1	96	theme	structural	283:292	arg1	characteristics					326:340	favourable structural, physicochemical and functional characteristics	272:340	favourable structural, physicochemical and functional characteristics	272:340	Polymer-based scaffolds have already gained popularity in many biomedical applications due to convenient routes for fabrication and favourable structural, physicochemical and functional characteristics.
31857577	1	0	theme	microorganisms	162:175	arg1	community					149:157	a huge community	142:157	a huge community of microorganisms that play essential roles in immune modulation and homeostasis maintenance	142:250	The gut microbiota represents a huge community of microorganisms that play essential roles in immune modulation and homeostasis maintenance.
31857577	7	1	theme	oral	1005:1008	arg1	delivery					1010:1017	oral delivery	1005:1017	oral delivery	1005:1017	We demonstrate their enhanced efficacies in oral delivery and treatment using two murine models of colitis.
31857577	8	2	theme	biointerfacial	1085:1098	arg1	self-assembly					1115:1127	biointerfacial supramolecular self-assembly	1085:1127	biointerfacial supramolecular self-assembly	1085:1127	We suggest that biointerfacial supramolecular self-assembly may provide a unique platform to generate advanced bacterial therapeutics for the treatment of various diseases.
31857577	7	3	theme	enhanced	982:989	arg1	efficacies					991:1000	their enhanced efficacies	976:1000	their enhanced efficacies in oral delivery and treatment	976:1031	We demonstrate their enhanced efficacies in oral delivery and treatment using two murine models of colitis.
31857577	8	4	theme	advanced	1171:1178	arg1	therapeutics					1190:1201	advanced bacterial therapeutics	1171:1201	advanced bacterial therapeutics for the treatment of various diseases	1171:1239	We suggest that biointerfacial supramolecular self-assembly may provide a unique platform to generate advanced bacterial therapeutics for the treatment of various diseases.
31857577	4	5	theme	gut	634:636	arg1	microbes					638:645	coat gut microbes	629:645	coat gut microbes	629:645	Here, we report a simple yet highly efficient method to coat gut microbes via biointerfacial supramolecular self-assembly.
31857577	7	6	from	efficacies	991:1000	arg1	treatment					1023:1031	treatment	1023:1031	treatment	1023:1031	We demonstrate their enhanced efficacies in oral delivery and treatment using two murine models of colitis.
31857577	7	6	from	efficacies	991:1000	arg1	delivery					1010:1017	oral delivery	1005:1017	oral delivery	1005:1017	We demonstrate their enhanced efficacies in oral delivery and treatment using two murine models of colitis.
31857577	7	7	theme	colitis	1060:1066	arg1	models					1050:1055	two murine models	1039:1055	two murine models of colitis	1039:1066	We demonstrate their enhanced efficacies in oral delivery and treatment using two murine models of colitis.
31857577	3	8	theme	gastrointestinal	549:564	arg1	tract					566:570	the gastrointestinal tract	545:570	the gastrointestinal tract	545:570	However, the development of oral bacterial therapeutics has been restricted by low bioavailability and limited retention in the gastrointestinal tract.
31857577	4	9	theme	coat	629:632	arg1	microbes					638:645	coat gut microbes	629:645	coat gut microbes	629:645	Here, we report a simple yet highly efficient method to coat gut microbes via biointerfacial supramolecular self-assembly.
31857577	6	10	theme	environmental	890:902	arg1	assaults					904:911	environmental assaults	890:911	environmental assaults	890:911	Bacteria coated with an extra self-assembled lipid membrane exhibit significantly improved survival against environmental assaults and almost unchanged viability and bioactivity.
31857577	0	11	theme	coated	54:59	arg1	bacteria					61:68	lipid membrane coated bacteria	39:68	lipid membrane coated bacteria	39:68	Biointerfacial self-assembly generates lipid membrane coated bacteria for enhanced oral delivery and treatment.
31857577	1	12	theme	essential	187:195	arg1	roles					197:201	essential roles	187:201	essential roles	187:201	The gut microbiota represents a huge community of microorganisms that play essential roles in immune modulation and homeostasis maintenance.
31857577	3	13	from	retention	532:540	arg1	tract					566:570	the gastrointestinal tract	545:570	the gastrointestinal tract	545:570	However, the development of oral bacterial therapeutics has been restricted by low bioavailability and limited retention in the gastrointestinal tract.
31857577	3	14	theme	therapeutics	464:475	arg1	development					434:444	the development	430:444	the development of oral bacterial therapeutics	430:475	However, the development of oral bacterial therapeutics has been restricted by low bioavailability and limited retention in the gastrointestinal tract.
31857577	0	15	theme	Biointerfacial	0:13	arg1	self-assembly					15:27	Biointerfacial self-assembly	0:27	Biointerfacial self-assembly	0:27	Biointerfacial self-assembly generates lipid membrane coated bacteria for enhanced oral delivery and treatment.
31857577	6	16	theme	unchanged	924:932	arg1	viability					934:942	viability	934:942	viability	934:942	Bacteria coated with an extra self-assembled lipid membrane exhibit significantly improved survival against environmental assaults and almost unchanged viability and bioactivity.
31857577	6	17	theme	improved	864:871	arg1	survival					873:880	significantly improved survival	850:880	significantly improved survival against environmental assaults and almost unchanged viability and bioactivity	850:958	Bacteria coated with an extra self-assembled lipid membrane exhibit significantly improved survival against environmental assaults and almost unchanged viability and bioactivity.
31857577	8	18	theme	unique	1143:1148	arg1	platform					1150:1157	a unique platform	1141:1157	a unique platform to generate advanced bacterial therapeutics for the treatment of various diseases	1141:1239	We suggest that biointerfacial supramolecular self-assembly may provide a unique platform to generate advanced bacterial therapeutics for the treatment of various diseases.
31857577	0	19	theme	lipid	39:43	arg1	bacteria					61:68	lipid membrane coated bacteria	39:68	lipid membrane coated bacteria	39:68	Biointerfacial self-assembly generates lipid membrane coated bacteria for enhanced oral delivery and treatment.
31857577	4	20	theme	simple	591:596	arg1	method					619:624	a simple yet highly efficient method	589:624	a simple yet highly efficient method to coat gut microbes	589:645	Here, we report a simple yet highly efficient method to coat gut microbes via biointerfacial supramolecular self-assembly.
31857577	5	21	theme	biocompatible	760:772	arg1	lipids					774:779	biocompatible lipids	760:779	biocompatible lipids	760:779	Coating can be performed within 15 min by simply vortexing with biocompatible lipids.
31857577	7	22	theme	murine	1043:1048	arg1	models					1050:1055	two murine models	1039:1055	two murine models of colitis	1039:1066	We demonstrate their enhanced efficacies in oral delivery and treatment using two murine models of colitis.
31857577	1	23	theme	immune	206:211	arg1	modulation					213:222	immune modulation	206:222	immune modulation	206:222	The gut microbiota represents a huge community of microorganisms that play essential roles in immune modulation and homeostasis maintenance.
31857577	4	24	theme	efficient	609:617	arg1	method					619:624	a simple yet highly efficient method	589:624	a simple yet highly efficient method to coat gut microbes	589:645	Here, we report a simple yet highly efficient method to coat gut microbes via biointerfacial supramolecular self-assembly.
31857577	6	25	theme	lipid	827:831	arg1	membrane					833:840	an extra self-assembled lipid membrane	803:840	an extra self-assembled lipid membrane	803:840	Bacteria coated with an extra self-assembled lipid membrane exhibit significantly improved survival against environmental assaults and almost unchanged viability and bioactivity.
31857577	1	26	theme	gut	116:118	arg1	microbiota					120:129	The gut microbiota	112:129	The gut microbiota	112:129	The gut microbiota represents a huge community of microorganisms that play essential roles in immune modulation and homeostasis maintenance.
31857577	3	27	theme	oral	449:452	arg1	therapeutics					464:475	oral bacterial therapeutics	449:475	oral bacterial therapeutics	449:475	However, the development of oral bacterial therapeutics has been restricted by low bioavailability and limited retention in the gastrointestinal tract.
31857577	8	28	theme	diseases	1232:1239	arg1	treatment					1211:1219	the treatment	1207:1219	the treatment of various diseases	1207:1239	We suggest that biointerfacial supramolecular self-assembly may provide a unique platform to generate advanced bacterial therapeutics for the treatment of various diseases.
31857577	0	29	theme	membrane	45:52	arg1	bacteria					61:68	lipid membrane coated bacteria	39:68	lipid membrane coated bacteria	39:68	Biointerfacial self-assembly generates lipid membrane coated bacteria for enhanced oral delivery and treatment.
31857577	3	30	theme	bacterial	454:462	arg1	therapeutics					464:475	oral bacterial therapeutics	449:475	oral bacterial therapeutics	449:475	However, the development of oral bacterial therapeutics has been restricted by low bioavailability and limited retention in the gastrointestinal tract.
31857577	2	31	theme	Microbiota	253:262	arg1	transplantation					264:278	Microbiota transplantation	253:278	Microbiota transplantation	253:278	Microbiota transplantation is an important approach to prevent and treat disease as it can inhibit pathogen colonization and positively modulate bacterial composition.
31857577	2	31	theme	Microbiota	253:262	arg1	approach					296:303	an important approach	283:303	an important approach to prevent and treat disease as it can inhibit pathogen colonization and positively modulate bacterial composition	283:418	Microbiota transplantation is an important approach to prevent and treat disease as it can inhibit pathogen colonization and positively modulate bacterial composition.
31857577	3	32	theme	low	500:502	arg1	bioavailability					504:518	low bioavailability	500:518	low bioavailability	500:518	However, the development of oral bacterial therapeutics has been restricted by low bioavailability and limited retention in the gastrointestinal tract.
31857577	8	33	theme	various	1224:1230	arg1	diseases					1232:1239	various diseases	1224:1239	various diseases	1224:1239	We suggest that biointerfacial supramolecular self-assembly may provide a unique platform to generate advanced bacterial therapeutics for the treatment of various diseases.
31857577	8	34	theme	bacterial	1180:1188	arg1	therapeutics					1190:1201	advanced bacterial therapeutics	1171:1201	advanced bacterial therapeutics for the treatment of various diseases	1171:1239	We suggest that biointerfacial supramolecular self-assembly may provide a unique platform to generate advanced bacterial therapeutics for the treatment of various diseases.
31857577	3	35	from	bioavailability	504:518	arg1	tract					566:570	the gastrointestinal tract	545:570	the gastrointestinal tract	545:570	However, the development of oral bacterial therapeutics has been restricted by low bioavailability and limited retention in the gastrointestinal tract.
31857577	6	36	theme	self-assembled	812:825	arg1	membrane					833:840	an extra self-assembled lipid membrane	803:840	an extra self-assembled lipid membrane	803:840	Bacteria coated with an extra self-assembled lipid membrane exhibit significantly improved survival against environmental assaults and almost unchanged viability and bioactivity.
31857577	2	37	theme	pathogen	352:359	arg1	colonization					361:372	pathogen colonization	352:372	pathogen colonization	352:372	Microbiota transplantation is an important approach to prevent and treat disease as it can inhibit pathogen colonization and positively modulate bacterial composition.
31857577	2	38	theme	important	286:294	arg1	transplantation					264:278	Microbiota transplantation	253:278	Microbiota transplantation	253:278	Microbiota transplantation is an important approach to prevent and treat disease as it can inhibit pathogen colonization and positively modulate bacterial composition.
31857577	2	38	theme	important	286:294	arg1	approach					296:303	an important approach	283:303	an important approach to prevent and treat disease as it can inhibit pathogen colonization and positively modulate bacterial composition	283:418	Microbiota transplantation is an important approach to prevent and treat disease as it can inhibit pathogen colonization and positively modulate bacterial composition.
31857577	6	39	theme	extra	806:810	arg1	membrane					833:840	an extra self-assembled lipid membrane	803:840	an extra self-assembled lipid membrane	803:840	Bacteria coated with an extra self-assembled lipid membrane exhibit significantly improved survival against environmental assaults and almost unchanged viability and bioactivity.
31857577	1	40	theme	huge	144:147	arg1	community					149:157	a huge community	142:157	a huge community of microorganisms that play essential roles in immune modulation and homeostasis maintenance	142:250	The gut microbiota represents a huge community of microorganisms that play essential roles in immune modulation and homeostasis maintenance.
31857577	3	41	theme	limited	524:530	arg1	retention					532:540	limited retention	524:540	limited retention	524:540	However, the development of oral bacterial therapeutics has been restricted by low bioavailability and limited retention in the gastrointestinal tract.
31857577	0	42	theme	oral	83:86	arg1	delivery					88:95	enhanced oral delivery	74:95	enhanced oral delivery	74:95	Biointerfacial self-assembly generates lipid membrane coated bacteria for enhanced oral delivery and treatment.
31857577	1	43	theme	homeostasis	228:238	arg1	maintenance					240:250	homeostasis maintenance	228:250	homeostasis maintenance	228:250	The gut microbiota represents a huge community of microorganisms that play essential roles in immune modulation and homeostasis maintenance.
31857577	8	44	theme	supramolecular	1100:1113	arg1	self-assembly					1115:1127	biointerfacial supramolecular self-assembly	1085:1127	biointerfacial supramolecular self-assembly	1085:1127	We suggest that biointerfacial supramolecular self-assembly may provide a unique platform to generate advanced bacterial therapeutics for the treatment of various diseases.
31857577	4	45	theme	supramolecular	666:679	arg1	self-assembly					681:693	biointerfacial supramolecular self-assembly	651:693	biointerfacial supramolecular self-assembly	651:693	Here, we report a simple yet highly efficient method to coat gut microbes via biointerfacial supramolecular self-assembly.
31857577	0	46	theme	enhanced	74:81	arg1	delivery					88:95	enhanced oral delivery	74:95	enhanced oral delivery	74:95	Biointerfacial self-assembly generates lipid membrane coated bacteria for enhanced oral delivery and treatment.
31857577	2	47	theme	bacterial	398:406	arg1	composition					408:418	bacterial composition	398:418	bacterial composition	398:418	Microbiota transplantation is an important approach to prevent and treat disease as it can inhibit pathogen colonization and positively modulate bacterial composition.
31857577	4	48	theme	biointerfacial	651:664	arg1	self-assembly					681:693	biointerfacial supramolecular self-assembly	651:693	biointerfacial supramolecular self-assembly	651:693	Here, we report a simple yet highly efficient method to coat gut microbes via biointerfacial supramolecular self-assembly.
30827149	4	0	theme	intergroup	942:951	arg1	variability					953:963	intergroup variability	942:963	intergroup variability	942:963	In most cases, in vitro dietary interventions caused microbiota-dependent modulations as a result of intergroup variability, but also specific changes in microbial groups were shared between the three microbiotas, highlighting specific diet-microbial taxa connections.
30827149	4	1	from	changes	984:990	arg1	groups					1005:1010	microbial groups	995:1010	microbial groups	995:1010	In most cases, in vitro dietary interventions caused microbiota-dependent modulations as a result of intergroup variability, but also specific changes in microbial groups were shared between the three microbiotas, highlighting specific diet-microbial taxa connections.
30827149	3	2	theme	gas	779:781	arg1	chromatography					783:796	gas chromatography	779:796	gas chromatography	779:796	Short- and branched-chain fatty acids production and microbiota composition were assessed by means of gas chromatography and 16S rRNA gene profiling, respectively.
30827149	2	3	theme	well-controlled	611:625	arg1	TNO					627:629	the well-controlled TNO	607:629	the well-controlled TNO in vitro model of the proximal colon (TIM-2)	607:674	We here explored the effects of Bacillus coagulans GBI-30, 6086 (BC30), β-glucans, and innovative whole-grain pastas, with or without these functional ingredients, on gut microbiota from three groups of children, presenting different susceptibility to type 1 diabetes, by using the well-controlled TNO in vitro model of the proximal colon (TIM-2).
30827149	2	4	theme	functional	469:478	arg1	ingredients					480:490	these functional ingredients	463:490	these functional ingredients	463:490	We here explored the effects of Bacillus coagulans GBI-30, 6086 (BC30), β-glucans, and innovative whole-grain pastas, with or without these functional ingredients, on gut microbiota from three groups of children, presenting different susceptibility to type 1 diabetes, by using the well-controlled TNO in vitro model of the proximal colon (TIM-2).
30827149	0	5	theme	in	89:90	arg1	model					98:102	TIM-2 in vitro model	83:102	TIM-2 in vitro model of the proximal colon	83:124	Effects of functional pasta ingredients on different gut microbiota as revealed by TIM-2 in vitro model of the proximal colon.
30827149	0	6	from	Effects	0:6	arg1	microbiota					57:66	different gut microbiota	43:66	different gut microbiota	43:66	Effects of functional pasta ingredients on different gut microbiota as revealed by TIM-2 in vitro model of the proximal colon.
30827149	1	7	from	imbalances	288:297	arg1	communities					316:326	gut microbial communities	302:326	gut microbial communities	302:326	Diet-related modulation of gut microbiota and its metabolic activity represents an intriguing research context, particularly in the case of disorders related to imbalances in gut microbial communities.
30827149	0	8	theme	TIM-2	83:87	arg1	model					98:102	TIM-2 in vitro model	83:102	TIM-2 in vitro model of the proximal colon	83:124	Effects of functional pasta ingredients on different gut microbiota as revealed by TIM-2 in vitro model of the proximal colon.
30827149	4	9	theme	variability	953:963	arg1	modulations					915:925	microbiota-dependent modulations	894:925	microbiota-dependent modulations	894:925	In most cases, in vitro dietary interventions caused microbiota-dependent modulations as a result of intergroup variability, but also specific changes in microbial groups were shared between the three microbiotas, highlighting specific diet-microbial taxa connections.
30827149	4	9	theme	variability	953:963	arg1	result					932:937	a result	930:937	a result of intergroup variability	930:963	In most cases, in vitro dietary interventions caused microbiota-dependent modulations as a result of intergroup variability, but also specific changes in microbial groups were shared between the three microbiotas, highlighting specific diet-microbial taxa connections.
30827149	2	10	dep	Bacillus	361:368	arg1	coagulans					370:378	coagulans	370:378	coagulans	370:378	We here explored the effects of Bacillus coagulans GBI-30, 6086 (BC30), β-glucans, and innovative whole-grain pastas, with or without these functional ingredients, on gut microbiota from three groups of children, presenting different susceptibility to type 1 diabetes, by using the well-controlled TNO in vitro model of the proximal colon (TIM-2).
30827149	0	11	dep	in	89:90	arg1	vitro					92:96	vitro	92:96	vitro	92:96	Effects of functional pasta ingredients on different gut microbiota as revealed by TIM-2 in vitro model of the proximal colon.
30827149	1	12	theme	gut	154:156	arg1	microbiota					158:167	gut microbiota	154:167	gut microbiota	154:167	Diet-related modulation of gut microbiota and its metabolic activity represents an intriguing research context, particularly in the case of disorders related to imbalances in gut microbial communities.
30827149	2	13	theme	innovative	416:425	arg1	pastas					439:444	innovative whole-grain pastas	416:444	innovative whole-grain pastas	416:444	We here explored the effects of Bacillus coagulans GBI-30, 6086 (BC30), β-glucans, and innovative whole-grain pastas, with or without these functional ingredients, on gut microbiota from three groups of children, presenting different susceptibility to type 1 diabetes, by using the well-controlled TNO in vitro model of the proximal colon (TIM-2).
30827149	1	14	theme	microbiota	158:167	arg1	modulation					140:149	Diet-related modulation	127:149	Diet-related modulation of gut microbiota and its metabolic activity	127:194	Diet-related modulation of gut microbiota and its metabolic activity represents an intriguing research context, particularly in the case of disorders related to imbalances in gut microbial communities.
30827149	2	15	theme	pastas	439:444	arg1	effects					350:356	the effects	346:356	the effects of Bacillus coagulans GBI-30, 6086 (BC30), β-glucans, and innovative whole-grain pastas, with or without these functional ingredients, on gut microbiota from three groups of children	346:539	We here explored the effects of Bacillus coagulans GBI-30, 6086 (BC30), β-glucans, and innovative whole-grain pastas, with or without these functional ingredients, on gut microbiota from three groups of children, presenting different susceptibility to type 1 diabetes, by using the well-controlled TNO in vitro model of the proximal colon (TIM-2).
30827149	2	16	theme	vitro	634:638	arg1	model					640:644	vitro model	634:644	vitro model of the proximal colon (TIM-2)	634:674	We here explored the effects of Bacillus coagulans GBI-30, 6086 (BC30), β-glucans, and innovative whole-grain pastas, with or without these functional ingredients, on gut microbiota from three groups of children, presenting different susceptibility to type 1 diabetes, by using the well-controlled TNO in vitro model of the proximal colon (TIM-2).
30827149	4	17	theme	microbiota-dependent	894:913	arg1	modulations					915:925	microbiota-dependent modulations	894:925	microbiota-dependent modulations	894:925	In most cases, in vitro dietary interventions caused microbiota-dependent modulations as a result of intergroup variability, but also specific changes in microbial groups were shared between the three microbiotas, highlighting specific diet-microbial taxa connections.
30827149	4	17	theme	microbiota-dependent	894:913	arg1	result					932:937	a result	930:937	a result of intergroup variability	930:963	In most cases, in vitro dietary interventions caused microbiota-dependent modulations as a result of intergroup variability, but also specific changes in microbial groups were shared between the three microbiotas, highlighting specific diet-microbial taxa connections.
30827149	4	18	theme	specific	975:982	arg1	changes					984:990	specific changes	975:990	specific changes in microbial groups	975:1010	In most cases, in vitro dietary interventions caused microbiota-dependent modulations as a result of intergroup variability, but also specific changes in microbial groups were shared between the three microbiotas, highlighting specific diet-microbial taxa connections.
30827149	3	19	theme	branched-chain	688:701	arg1	acids					709:713	branched-chain fatty acids	688:713	branched-chain fatty acids	688:713	Short- and branched-chain fatty acids production and microbiota composition were assessed by means of gas chromatography and 16S rRNA gene profiling, respectively.
30827149	2	20	theme	type	581:584	arg1	diabetes					588:595	type 1 diabetes	581:595	type 1 diabetes	581:595	We here explored the effects of Bacillus coagulans GBI-30, 6086 (BC30), β-glucans, and innovative whole-grain pastas, with or without these functional ingredients, on gut microbiota from three groups of children, presenting different susceptibility to type 1 diabetes, by using the well-controlled TNO in vitro model of the proximal colon (TIM-2).
30827149	4	21	theme	specific	1068:1075	arg1	connections					1097:1107	specific diet-microbial taxa connections	1068:1107	specific diet-microbial taxa connections	1068:1107	In most cases, in vitro dietary interventions caused microbiota-dependent modulations as a result of intergroup variability, but also specific changes in microbial groups were shared between the three microbiotas, highlighting specific diet-microbial taxa connections.
30827149	2	22	theme	Bacillus	361:368	arg1	GBI-30					380:385	Bacillus coagulans GBI-30	361:385	Bacillus coagulans GBI-30	361:385	We here explored the effects of Bacillus coagulans GBI-30, 6086 (BC30), β-glucans, and innovative whole-grain pastas, with or without these functional ingredients, on gut microbiota from three groups of children, presenting different susceptibility to type 1 diabetes, by using the well-controlled TNO in vitro model of the proximal colon (TIM-2).
30827149	0	23	theme	pasta	22:26	arg1	ingredients					28:38	functional pasta ingredients	11:38	functional pasta ingredients	11:38	Effects of functional pasta ingredients on different gut microbiota as revealed by TIM-2 in vitro model of the proximal colon.
30827149	1	24	theme	disorders	267:275	arg1	case					259:262	the case	255:262	the case of disorders related to imbalances in gut microbial communities	255:326	Diet-related modulation of gut microbiota and its metabolic activity represents an intriguing research context, particularly in the case of disorders related to imbalances in gut microbial communities.
30827149	2	25	from	effects	350:356	arg1	microbiota					500:509	gut microbiota	496:509	gut microbiota from three groups of children	496:539	We here explored the effects of Bacillus coagulans GBI-30, 6086 (BC30), β-glucans, and innovative whole-grain pastas, with or without these functional ingredients, on gut microbiota from three groups of children, presenting different susceptibility to type 1 diabetes, by using the well-controlled TNO in vitro model of the proximal colon (TIM-2).
30827149	1	26	theme	metabolic	177:185	arg1	activity					187:194	its metabolic activity	173:194	its metabolic activity	173:194	Diet-related modulation of gut microbiota and its metabolic activity represents an intriguing research context, particularly in the case of disorders related to imbalances in gut microbial communities.
30827149	0	27	theme	functional	11:20	arg1	ingredients					28:38	functional pasta ingredients	11:38	functional pasta ingredients	11:38	Effects of functional pasta ingredients on different gut microbiota as revealed by TIM-2 in vitro model of the proximal colon.
30827149	1	28	theme	related	277:283	arg1	disorders					267:275	disorders	267:275	disorders related to imbalances in gut microbial communities	267:326	Diet-related modulation of gut microbiota and its metabolic activity represents an intriguing research context, particularly in the case of disorders related to imbalances in gut microbial communities.
30827149	4	29	theme	in	856:857	arg1	interventions					873:885	in vitro dietary interventions	856:885	in vitro dietary interventions	856:885	In most cases, in vitro dietary interventions caused microbiota-dependent modulations as a result of intergroup variability, but also specific changes in microbial groups were shared between the three microbiotas, highlighting specific diet-microbial taxa connections.
30827149	4	30	theme	dietary	865:871	arg1	interventions					873:885	in vitro dietary interventions	856:885	in vitro dietary interventions	856:885	In most cases, in vitro dietary interventions caused microbiota-dependent modulations as a result of intergroup variability, but also specific changes in microbial groups were shared between the three microbiotas, highlighting specific diet-microbial taxa connections.
30827149	4	31	theme	diet-microbial	1077:1090	arg1	connections					1097:1107	specific diet-microbial taxa connections	1068:1107	specific diet-microbial taxa connections	1068:1107	In most cases, in vitro dietary interventions caused microbiota-dependent modulations as a result of intergroup variability, but also specific changes in microbial groups were shared between the three microbiotas, highlighting specific diet-microbial taxa connections.
30827149	4	32	theme	taxa	1092:1095	arg1	connections					1097:1107	specific diet-microbial taxa connections	1068:1107	specific diet-microbial taxa connections	1068:1107	In most cases, in vitro dietary interventions caused microbiota-dependent modulations as a result of intergroup variability, but also specific changes in microbial groups were shared between the three microbiotas, highlighting specific diet-microbial taxa connections.
30827149	1	33	theme	activity	187:194	arg1	modulation					140:149	Diet-related modulation	127:149	Diet-related modulation of gut microbiota and its metabolic activity	127:194	Diet-related modulation of gut microbiota and its metabolic activity represents an intriguing research context, particularly in the case of disorders related to imbalances in gut microbial communities.
30827149	0	34	theme	colon	120:124	arg1	model					98:102	TIM-2 in vitro model	83:102	TIM-2 in vitro model of the proximal colon	83:124	Effects of functional pasta ingredients on different gut microbiota as revealed by TIM-2 in vitro model of the proximal colon.
30827149	3	35	theme	16S	802:804	arg1	rRNA					806:809	16S rRNA	802:809	16S rRNA	802:809	Short- and branched-chain fatty acids production and microbiota composition were assessed by means of gas chromatography and 16S rRNA gene profiling, respectively.
30827149	0	36	theme	ingredients	28:38	arg1	Effects					0:6	Effects	0:6	Effects of functional pasta ingredients on different gut microbiota	0:66	Effects of functional pasta ingredients on different gut microbiota as revealed by TIM-2 in vitro model of the proximal colon.
30827149	0	37	theme	proximal	111:118	arg1	colon					120:124	the proximal colon	107:124	the proximal colon	107:124	Effects of functional pasta ingredients on different gut microbiota as revealed by TIM-2 in vitro model of the proximal colon.
30827149	2	38	theme	β-glucans	401:409	arg1	effects					350:356	the effects	346:356	the effects of Bacillus coagulans GBI-30, 6086 (BC30), β-glucans, and innovative whole-grain pastas, with or without these functional ingredients, on gut microbiota from three groups of children	346:539	We here explored the effects of Bacillus coagulans GBI-30, 6086 (BC30), β-glucans, and innovative whole-grain pastas, with or without these functional ingredients, on gut microbiota from three groups of children, presenting different susceptibility to type 1 diabetes, by using the well-controlled TNO in vitro model of the proximal colon (TIM-2).
30827149	2	39	theme	gut	496:498	arg1	microbiota					500:509	gut microbiota	496:509	gut microbiota from three groups of children	496:539	We here explored the effects of Bacillus coagulans GBI-30, 6086 (BC30), β-glucans, and innovative whole-grain pastas, with or without these functional ingredients, on gut microbiota from three groups of children, presenting different susceptibility to type 1 diabetes, by using the well-controlled TNO in vitro model of the proximal colon (TIM-2).
30827149	2	40	theme	6086	388:391	arg1	effects					350:356	the effects	346:356	the effects of Bacillus coagulans GBI-30, 6086 (BC30), β-glucans, and innovative whole-grain pastas, with or without these functional ingredients, on gut microbiota from three groups of children	346:539	We here explored the effects of Bacillus coagulans GBI-30, 6086 (BC30), β-glucans, and innovative whole-grain pastas, with or without these functional ingredients, on gut microbiota from three groups of children, presenting different susceptibility to type 1 diabetes, by using the well-controlled TNO in vitro model of the proximal colon (TIM-2).
30827149	0	41	theme	gut	53:55	arg1	microbiota					57:66	different gut microbiota	43:66	different gut microbiota	43:66	Effects of functional pasta ingredients on different gut microbiota as revealed by TIM-2 in vitro model of the proximal colon.
30827149	3	42	theme	microbiota	730:739	arg1	composition					741:751	microbiota composition	730:751	microbiota composition	730:751	Short- and branched-chain fatty acids production and microbiota composition were assessed by means of gas chromatography and 16S rRNA gene profiling, respectively.
30827149	4	43	theme	most	844:847	arg1	cases					849:853	most cases	844:853	most cases	844:853	In most cases, in vitro dietary interventions caused microbiota-dependent modulations as a result of intergroup variability, but also specific changes in microbial groups were shared between the three microbiotas, highlighting specific diet-microbial taxa connections.
30827149	3	44	theme	fatty	703:707	arg1	acids					709:713	branched-chain fatty acids	688:713	branched-chain fatty acids	688:713	Short- and branched-chain fatty acids production and microbiota composition were assessed by means of gas chromatography and 16S rRNA gene profiling, respectively.
30827149	2	45	theme	proximal	653:660	arg1	TIM-2					669:673	TIM-2	669:673	TIM-2	669:673	We here explored the effects of Bacillus coagulans GBI-30, 6086 (BC30), β-glucans, and innovative whole-grain pastas, with or without these functional ingredients, on gut microbiota from three groups of children, presenting different susceptibility to type 1 diabetes, by using the well-controlled TNO in vitro model of the proximal colon (TIM-2).
30827149	2	45	theme	proximal	653:660	arg1	colon					662:666	the proximal colon	649:666	the proximal colon (TIM-2)	649:674	We here explored the effects of Bacillus coagulans GBI-30, 6086 (BC30), β-glucans, and innovative whole-grain pastas, with or without these functional ingredients, on gut microbiota from three groups of children, presenting different susceptibility to type 1 diabetes, by using the well-controlled TNO in vitro model of the proximal colon (TIM-2).
30827149	0	46	theme	different	43:51	arg1	microbiota					57:66	different gut microbiota	43:66	different gut microbiota	43:66	Effects of functional pasta ingredients on different gut microbiota as revealed by TIM-2 in vitro model of the proximal colon.
30827149	1	47	theme	gut	302:304	arg1	communities					316:326	gut microbial communities	302:326	gut microbial communities	302:326	Diet-related modulation of gut microbiota and its metabolic activity represents an intriguing research context, particularly in the case of disorders related to imbalances in gut microbial communities.
30827149	2	48	theme	children	532:539	arg1	children					532:539	children	532:539	children	532:539	We here explored the effects of Bacillus coagulans GBI-30, 6086 (BC30), β-glucans, and innovative whole-grain pastas, with or without these functional ingredients, on gut microbiota from three groups of children, presenting different susceptibility to type 1 diabetes, by using the well-controlled TNO in vitro model of the proximal colon (TIM-2).
30827149	2	48	theme	children	532:539	arg1	groups					522:527	three groups	516:527	three groups of children	516:539	We here explored the effects of Bacillus coagulans GBI-30, 6086 (BC30), β-glucans, and innovative whole-grain pastas, with or without these functional ingredients, on gut microbiota from three groups of children, presenting different susceptibility to type 1 diabetes, by using the well-controlled TNO in vitro model of the proximal colon (TIM-2).
30827149	2	49	theme	different	553:561	arg1	susceptibility					563:576	different susceptibility	553:576	different susceptibility to type 1 diabetes	553:595	We here explored the effects of Bacillus coagulans GBI-30, 6086 (BC30), β-glucans, and innovative whole-grain pastas, with or without these functional ingredients, on gut microbiota from three groups of children, presenting different susceptibility to type 1 diabetes, by using the well-controlled TNO in vitro model of the proximal colon (TIM-2).
30827149	1	50	theme	microbial	306:314	arg1	communities					316:326	gut microbial communities	302:326	gut microbial communities	302:326	Diet-related modulation of gut microbiota and its metabolic activity represents an intriguing research context, particularly in the case of disorders related to imbalances in gut microbial communities.
30827149	2	51	from	groups	522:527	arg1	microbiota					500:509	gut microbiota	496:509	gut microbiota from three groups of children	496:539	We here explored the effects of Bacillus coagulans GBI-30, 6086 (BC30), β-glucans, and innovative whole-grain pastas, with or without these functional ingredients, on gut microbiota from three groups of children, presenting different susceptibility to type 1 diabetes, by using the well-controlled TNO in vitro model of the proximal colon (TIM-2).
30827149	4	52	dep	in	856:857	arg1	vitro					859:863	vitro	859:863	vitro	859:863	In most cases, in vitro dietary interventions caused microbiota-dependent modulations as a result of intergroup variability, but also specific changes in microbial groups were shared between the three microbiotas, highlighting specific diet-microbial taxa connections.
30827149	3	53	theme	gene	811:814	arg1	profiling					816:824	gene profiling	811:824	gene profiling	811:824	Short- and branched-chain fatty acids production and microbiota composition were assessed by means of gas chromatography and 16S rRNA gene profiling, respectively.
30827149	1	54	theme	intriguing	210:219	arg1	context					230:236	an intriguing research context	207:236	an intriguing research context	207:236	Diet-related modulation of gut microbiota and its metabolic activity represents an intriguing research context, particularly in the case of disorders related to imbalances in gut microbial communities.
30827149	2	55	theme	colon	662:666	arg1	model					640:644	vitro model	634:644	vitro model of the proximal colon (TIM-2)	634:674	We here explored the effects of Bacillus coagulans GBI-30, 6086 (BC30), β-glucans, and innovative whole-grain pastas, with or without these functional ingredients, on gut microbiota from three groups of children, presenting different susceptibility to type 1 diabetes, by using the well-controlled TNO in vitro model of the proximal colon (TIM-2).
30827149	2	56	from	TNO	627:629	arg1	model					640:644	vitro model	634:644	vitro model of the proximal colon (TIM-2)	634:674	We here explored the effects of Bacillus coagulans GBI-30, 6086 (BC30), β-glucans, and innovative whole-grain pastas, with or without these functional ingredients, on gut microbiota from three groups of children, presenting different susceptibility to type 1 diabetes, by using the well-controlled TNO in vitro model of the proximal colon (TIM-2).
30827149	1	57	theme	research	221:228	arg1	context					230:236	an intriguing research context	207:236	an intriguing research context	207:236	Diet-related modulation of gut microbiota and its metabolic activity represents an intriguing research context, particularly in the case of disorders related to imbalances in gut microbial communities.
30827149	3	58	dep	chromatography	783:796	arg1	profiling					816:824	gene profiling	811:824	gene profiling	811:824	Short- and branched-chain fatty acids production and microbiota composition were assessed by means of gas chromatography and 16S rRNA gene profiling, respectively.
30827149	2	59	theme	GBI-30	380:385	arg1	effects					350:356	the effects	346:356	the effects of Bacillus coagulans GBI-30, 6086 (BC30), β-glucans, and innovative whole-grain pastas, with or without these functional ingredients, on gut microbiota from three groups of children	346:539	We here explored the effects of Bacillus coagulans GBI-30, 6086 (BC30), β-glucans, and innovative whole-grain pastas, with or without these functional ingredients, on gut microbiota from three groups of children, presenting different susceptibility to type 1 diabetes, by using the well-controlled TNO in vitro model of the proximal colon (TIM-2).
30827149	2	60	theme	whole-grain	427:437	arg1	pastas					439:444	innovative whole-grain pastas	416:444	innovative whole-grain pastas	416:444	We here explored the effects of Bacillus coagulans GBI-30, 6086 (BC30), β-glucans, and innovative whole-grain pastas, with or without these functional ingredients, on gut microbiota from three groups of children, presenting different susceptibility to type 1 diabetes, by using the well-controlled TNO in vitro model of the proximal colon (TIM-2).
30827149	3	61	dep	Short-	677:682	arg1	production					715:724	production	715:724	production	715:724	Short- and branched-chain fatty acids production and microbiota composition were assessed by means of gas chromatography and 16S rRNA gene profiling, respectively.
30827149	3	61	dep	Short-	677:682	arg1	composition					741:751	microbiota composition	730:751	microbiota composition	730:751	Short- and branched-chain fatty acids production and microbiota composition were assessed by means of gas chromatography and 16S rRNA gene profiling, respectively.
30827149	4	62	theme	microbial	995:1003	arg1	groups					1005:1010	microbial groups	995:1010	microbial groups	995:1010	In most cases, in vitro dietary interventions caused microbiota-dependent modulations as a result of intergroup variability, but also specific changes in microbial groups were shared between the three microbiotas, highlighting specific diet-microbial taxa connections.
30827149	1	63	theme	Diet-related	127:138	arg1	modulation					140:149	Diet-related modulation	127:149	Diet-related modulation of gut microbiota and its metabolic activity	127:194	Diet-related modulation of gut microbiota and its metabolic activity represents an intriguing research context, particularly in the case of disorders related to imbalances in gut microbial communities.
31500015	0	0	theme	copper	96:101	arg1	release					85:91	pH-responsive release	71:91	pH-responsive release of copper and its bioactivity	71:121	Electrophoretic deposition of GHK-Cu loaded MSN-chitosan coatings with pH-responsive release of copper and its bioactivity.
31500015	1	1	theme	infections	336:345	arg1	incidence					310:318	the incidence	306:318	the incidence of post-surgery infections	306:345	Despite the fact that titanium has been widely applied in the replacement of bone defects, prosthesis failure still occurred because of the lack of adequate bone-bonding ability and the incidence of post-surgery infections.
31500015	1	1	theme	infections	336:345	arg1	lack					264:267	the lack	260:267	the lack of adequate bone-bonding ability	260:300	Despite the fact that titanium has been widely applied in the replacement of bone defects, prosthesis failure still occurred because of the lack of adequate bone-bonding ability and the incidence of post-surgery infections.
31500015	5	2	from	surface	985:991	arg1	activity					949:956	The functional activity	934:956	The functional activity of Cu2+ releasing from the surface	934:991	The functional activity of Cu2+ releasing from the surface was dependent on the pH value of the titanium surface.
31500015	5	2	from	surface	985:991	arg1	dependent					997:1005	dependent	997:1005	dependent	997:1005	The functional activity of Cu2+ releasing from the surface was dependent on the pH value of the titanium surface.
31500015	2	3	theme	biomedical	499:508	arg1	application					510:520	biomedical application	499:520	biomedical application	499:520	Concentration-dependent effects of therapeutic copper ions (Cu2+) for antibacterial and osteogenic activity have been well-established in the field of biomedical application.
31500015	2	4	from	well-established	466:481	arg1	field					490:494	the field	486:494	the field of biomedical application	486:520	Concentration-dependent effects of therapeutic copper ions (Cu2+) for antibacterial and osteogenic activity have been well-established in the field of biomedical application.
31500015	2	5	theme	application	510:520	arg1	field					490:494	the field	486:494	the field of biomedical application	486:520	Concentration-dependent effects of therapeutic copper ions (Cu2+) for antibacterial and osteogenic activity have been well-established in the field of biomedical application.
31500015	6	6	theme	Cu2+	1084:1087	arg1	release					1073:1079	the controllable release	1056:1079	the controllable release of Cu2+	1056:1087	Through the controllable release of Cu2+, the coating achieved not only inhibited adhesion of bacteria but also had good cytocompatibility.
31500015	3	7	theme	silica	561:566	arg1	MSN					583:585	MSN	583:585	MSN	583:585	In this study, we prepared mesoporous silica nanoparticles (MSN) and MSN-COOH with uniform sphere size (~100 nm) and developed multifunctional chitosan coatings loaded with MSN@GHK-Cu (glycyl-L-histidyl-l-lysine-Cu2+) as a suitable strategy by electrophoretic deposition (EPD).
31500015	3	7	theme	silica	561:566	arg1	nanoparticles					568:580	mesoporous silica nanoparticles	550:580	mesoporous silica nanoparticles (MSN)	550:586	In this study, we prepared mesoporous silica nanoparticles (MSN) and MSN-COOH with uniform sphere size (~100 nm) and developed multifunctional chitosan coatings loaded with MSN@GHK-Cu (glycyl-L-histidyl-l-lysine-Cu2+) as a suitable strategy by electrophoretic deposition (EPD).
31500015	3	8	theme	@	699:699	arg1	glycyl-L-histidyl-l-lysine-Cu2+					708:738	glycyl-L-histidyl-l-lysine-Cu2+	708:738	glycyl-L-histidyl-l-lysine-Cu2+	708:738	In this study, we prepared mesoporous silica nanoparticles (MSN) and MSN-COOH with uniform sphere size (~100 nm) and developed multifunctional chitosan coatings loaded with MSN@GHK-Cu (glycyl-L-histidyl-l-lysine-Cu2+) as a suitable strategy by electrophoretic deposition (EPD).
31500015	3	8	theme	@	699:699	arg1	GHK-Cu					700:705	MSN@GHK-Cu	696:705	MSN@GHK-Cu (glycyl-L-histidyl-l-lysine-Cu2+)	696:739	In this study, we prepared mesoporous silica nanoparticles (MSN) and MSN-COOH with uniform sphere size (~100 nm) and developed multifunctional chitosan coatings loaded with MSN@GHK-Cu (glycyl-L-histidyl-l-lysine-Cu2+) as a suitable strategy by electrophoretic deposition (EPD).
31500015	6	9	theme	good	1164:1167	arg1	cytocompatibility					1169:1185	good cytocompatibility	1164:1185	good cytocompatibility	1164:1185	Through the controllable release of Cu2+, the coating achieved not only inhibited adhesion of bacteria but also had good cytocompatibility.
31500015	3	10	theme	suitable	746:753	arg1	strategy					755:762	a suitable strategy	744:762	a suitable strategy by electrophoretic deposition (EPD)	744:798	In this study, we prepared mesoporous silica nanoparticles (MSN) and MSN-COOH with uniform sphere size (~100 nm) and developed multifunctional chitosan coatings loaded with MSN@GHK-Cu (glycyl-L-histidyl-l-lysine-Cu2+) as a suitable strategy by electrophoretic deposition (EPD).
31500015	3	10	theme	suitable	746:753	arg1	coatings					675:682	multifunctional chitosan coatings	650:682	multifunctional chitosan coatings loaded with MSN@GHK-Cu (glycyl-L-histidyl-l-lysine-Cu2+)	650:739	In this study, we prepared mesoporous silica nanoparticles (MSN) and MSN-COOH with uniform sphere size (~100 nm) and developed multifunctional chitosan coatings loaded with MSN@GHK-Cu (glycyl-L-histidyl-l-lysine-Cu2+) as a suitable strategy by electrophoretic deposition (EPD).
31500015	6	11	theme	controllable	1060:1071	arg1	release					1073:1079	the controllable release	1056:1079	the controllable release of Cu2+	1056:1087	Through the controllable release of Cu2+, the coating achieved not only inhibited adhesion of bacteria but also had good cytocompatibility.
31500015	0	12	theme	bioactivity	111:121	arg1	release					85:91	pH-responsive release	71:91	pH-responsive release of copper and its bioactivity	71:121	Electrophoretic deposition of GHK-Cu loaded MSN-chitosan coatings with pH-responsive release of copper and its bioactivity.
31500015	3	13	theme	mesoporous	550:559	arg1	MSN					583:585	MSN	583:585	MSN	583:585	In this study, we prepared mesoporous silica nanoparticles (MSN) and MSN-COOH with uniform sphere size (~100 nm) and developed multifunctional chitosan coatings loaded with MSN@GHK-Cu (glycyl-L-histidyl-l-lysine-Cu2+) as a suitable strategy by electrophoretic deposition (EPD).
31500015	3	13	theme	mesoporous	550:559	arg1	nanoparticles					568:580	mesoporous silica nanoparticles	550:580	mesoporous silica nanoparticles (MSN)	550:586	In this study, we prepared mesoporous silica nanoparticles (MSN) and MSN-COOH with uniform sphere size (~100 nm) and developed multifunctional chitosan coatings loaded with MSN@GHK-Cu (glycyl-L-histidyl-l-lysine-Cu2+) as a suitable strategy by electrophoretic deposition (EPD).
31500015	7	14	theme	drug	1327:1330	arg1	delivery					1307:1314	the controlled delivery	1292:1314	the controlled delivery in situ of drug or other biomolecules	1292:1352	The coating based on EPD technique could be considered as a promising surface modification approach for the controlled delivery in situ of drug or other biomolecules.
31500015	3	15	theme	chitosan	666:673	arg1	strategy					755:762	a suitable strategy	744:762	a suitable strategy by electrophoretic deposition (EPD)	744:798	In this study, we prepared mesoporous silica nanoparticles (MSN) and MSN-COOH with uniform sphere size (~100 nm) and developed multifunctional chitosan coatings loaded with MSN@GHK-Cu (glycyl-L-histidyl-l-lysine-Cu2+) as a suitable strategy by electrophoretic deposition (EPD).
31500015	3	15	theme	chitosan	666:673	arg1	coatings					675:682	multifunctional chitosan coatings	650:682	multifunctional chitosan coatings loaded with MSN@GHK-Cu (glycyl-L-histidyl-l-lysine-Cu2+)	650:739	In this study, we prepared mesoporous silica nanoparticles (MSN) and MSN-COOH with uniform sphere size (~100 nm) and developed multifunctional chitosan coatings loaded with MSN@GHK-Cu (glycyl-L-histidyl-l-lysine-Cu2+) as a suitable strategy by electrophoretic deposition (EPD).
31500015	2	16	from	field	490:494	arg1	well-established					466:481	well-established	466:481	well-established	466:481	Concentration-dependent effects of therapeutic copper ions (Cu2+) for antibacterial and osteogenic activity have been well-established in the field of biomedical application.
31500015	7	17	theme	EPD	1209:1211	arg1	technique					1213:1221	EPD technique	1209:1221	EPD technique	1209:1221	The coating based on EPD technique could be considered as a promising surface modification approach for the controlled delivery in situ of drug or other biomolecules.
31500015	5	18	theme	functional	938:947	arg1	activity					949:956	The functional activity	934:956	The functional activity of Cu2+ releasing from the surface	934:991	The functional activity of Cu2+ releasing from the surface was dependent on the pH value of the titanium surface.
31500015	5	18	theme	functional	938:947	arg1	dependent					997:1005	dependent	997:1005	dependent	997:1005	The functional activity of Cu2+ releasing from the surface was dependent on the pH value of the titanium surface.
31500015	4	19	dep	microstructure	805:818	arg1	The					801:803	The	801:803	The	801:803	The microstructure and composition of the coating were comprehensively characterized by using SEM, XRD, FTIR, and TEM, respectively.
31500015	0	20	theme	Electrophoretic	0:14	arg1	deposition					16:25	Electrophoretic deposition	0:25	Electrophoretic deposition of GHK-Cu	0:35	Electrophoretic deposition of GHK-Cu loaded MSN-chitosan coatings with pH-responsive release of copper and its bioactivity.
31500015	3	21	theme	electrophoretic	767:781	arg1	EPD					795:797	EPD	795:797	EPD	795:797	In this study, we prepared mesoporous silica nanoparticles (MSN) and MSN-COOH with uniform sphere size (~100 nm) and developed multifunctional chitosan coatings loaded with MSN@GHK-Cu (glycyl-L-histidyl-l-lysine-Cu2+) as a suitable strategy by electrophoretic deposition (EPD).
31500015	3	21	theme	electrophoretic	767:781	arg1	deposition					783:792	electrophoretic deposition	767:792	electrophoretic deposition (EPD)	767:798	In this study, we prepared mesoporous silica nanoparticles (MSN) and MSN-COOH with uniform sphere size (~100 nm) and developed multifunctional chitosan coatings loaded with MSN@GHK-Cu (glycyl-L-histidyl-l-lysine-Cu2+) as a suitable strategy by electrophoretic deposition (EPD).
31500015	6	22	theme	bacteria	1142:1149	arg1	adhesion					1130:1137	adhesion	1130:1137	adhesion of bacteria	1130:1149	Through the controllable release of Cu2+, the coating achieved not only inhibited adhesion of bacteria but also had good cytocompatibility.
31500015	2	23	theme	ions	402:405	arg1	effects					372:378	Concentration-dependent effects	348:378	Concentration-dependent effects of therapeutic copper ions (Cu2+) for antibacterial and osteogenic activity	348:454	Concentration-dependent effects of therapeutic copper ions (Cu2+) for antibacterial and osteogenic activity have been well-established in the field of biomedical application.
31500015	0	24	theme	GHK-Cu	30:35	arg1	deposition					16:25	Electrophoretic deposition	0:25	Electrophoretic deposition of GHK-Cu	0:35	Electrophoretic deposition of GHK-Cu loaded MSN-chitosan coatings with pH-responsive release of copper and its bioactivity.
31500015	1	25	theme	adequate	272:279	arg1	ability					294:300	adequate bone-bonding ability	272:300	adequate bone-bonding ability	272:300	Despite the fact that titanium has been widely applied in the replacement of bone defects, prosthesis failure still occurred because of the lack of adequate bone-bonding ability and the incidence of post-surgery infections.
31500015	5	26	theme	pH	1014:1015	arg1	value					1017:1021	the pH value	1010:1021	the pH value of the titanium surface	1010:1045	The functional activity of Cu2+ releasing from the surface was dependent on the pH value of the titanium surface.
31500015	2	27	theme	osteogenic	436:445	arg1	activity					447:454	antibacterial and osteogenic activity	418:454	antibacterial and osteogenic activity	418:454	Concentration-dependent effects of therapeutic copper ions (Cu2+) for antibacterial and osteogenic activity have been well-established in the field of biomedical application.
31500015	1	28	theme	bone-bonding	281:292	arg1	ability					294:300	adequate bone-bonding ability	272:300	adequate bone-bonding ability	272:300	Despite the fact that titanium has been widely applied in the replacement of bone defects, prosthesis failure still occurred because of the lack of adequate bone-bonding ability and the incidence of post-surgery infections.
31500015	3	29	theme	multifunctional	650:664	arg1	strategy					755:762	a suitable strategy	744:762	a suitable strategy by electrophoretic deposition (EPD)	744:798	In this study, we prepared mesoporous silica nanoparticles (MSN) and MSN-COOH with uniform sphere size (~100 nm) and developed multifunctional chitosan coatings loaded with MSN@GHK-Cu (glycyl-L-histidyl-l-lysine-Cu2+) as a suitable strategy by electrophoretic deposition (EPD).
31500015	3	29	theme	multifunctional	650:664	arg1	coatings					675:682	multifunctional chitosan coatings	650:682	multifunctional chitosan coatings loaded with MSN@GHK-Cu (glycyl-L-histidyl-l-lysine-Cu2+)	650:739	In this study, we prepared mesoporous silica nanoparticles (MSN) and MSN-COOH with uniform sphere size (~100 nm) and developed multifunctional chitosan coatings loaded with MSN@GHK-Cu (glycyl-L-histidyl-l-lysine-Cu2+) as a suitable strategy by electrophoretic deposition (EPD).
31500015	5	30	theme	Cu2+	961:964	arg1	activity					949:956	The functional activity	934:956	The functional activity of Cu2+ releasing from the surface	934:991	The functional activity of Cu2+ releasing from the surface was dependent on the pH value of the titanium surface.
31500015	5	30	theme	Cu2+	961:964	arg1	dependent					997:1005	dependent	997:1005	dependent	997:1005	The functional activity of Cu2+ releasing from the surface was dependent on the pH value of the titanium surface.
31500015	1	31	theme	bone	201:204	arg1	defects					206:212	bone defects	201:212	bone defects	201:212	Despite the fact that titanium has been widely applied in the replacement of bone defects, prosthesis failure still occurred because of the lack of adequate bone-bonding ability and the incidence of post-surgery infections.
31500015	0	32	theme	MSN-chitosan	44:55	arg1	coatings					57:64	MSN-chitosan coatings	44:64	MSN-chitosan coatings	44:64	Electrophoretic deposition of GHK-Cu loaded MSN-chitosan coatings with pH-responsive release of copper and its bioactivity.
31500015	1	33	theme	ability	294:300	arg1	incidence					310:318	the incidence	306:318	the incidence of post-surgery infections	306:345	Despite the fact that titanium has been widely applied in the replacement of bone defects, prosthesis failure still occurred because of the lack of adequate bone-bonding ability and the incidence of post-surgery infections.
31500015	1	33	theme	ability	294:300	arg1	lack					264:267	the lack	260:267	the lack of adequate bone-bonding ability	260:300	Despite the fact that titanium has been widely applied in the replacement of bone defects, prosthesis failure still occurred because of the lack of adequate bone-bonding ability and the incidence of post-surgery infections.
31500015	1	34	theme	defects	206:212	arg1	replacement					186:196	the replacement	182:196	the replacement of bone defects	182:212	Despite the fact that titanium has been widely applied in the replacement of bone defects, prosthesis failure still occurred because of the lack of adequate bone-bonding ability and the incidence of post-surgery infections.
31500015	3	35	theme	uniform	606:612	arg1	~100 nm					627:633	~100 nm	627:633	~100 nm	627:633	In this study, we prepared mesoporous silica nanoparticles (MSN) and MSN-COOH with uniform sphere size (~100 nm) and developed multifunctional chitosan coatings loaded with MSN@GHK-Cu (glycyl-L-histidyl-l-lysine-Cu2+) as a suitable strategy by electrophoretic deposition (EPD).
31500015	3	35	theme	uniform	606:612	arg1	size					621:624	uniform sphere size	606:624	uniform sphere size (~100 nm)	606:634	In this study, we prepared mesoporous silica nanoparticles (MSN) and MSN-COOH with uniform sphere size (~100 nm) and developed multifunctional chitosan coatings loaded with MSN@GHK-Cu (glycyl-L-histidyl-l-lysine-Cu2+) as a suitable strategy by electrophoretic deposition (EPD).
31500015	2	36	theme	antibacterial	418:430	arg1	activity					447:454	antibacterial and osteogenic activity	418:454	antibacterial and osteogenic activity	418:454	Concentration-dependent effects of therapeutic copper ions (Cu2+) for antibacterial and osteogenic activity have been well-established in the field of biomedical application.
31500015	3	37	theme	sphere	614:619	arg1	~100 nm					627:633	~100 nm	627:633	~100 nm	627:633	In this study, we prepared mesoporous silica nanoparticles (MSN) and MSN-COOH with uniform sphere size (~100 nm) and developed multifunctional chitosan coatings loaded with MSN@GHK-Cu (glycyl-L-histidyl-l-lysine-Cu2+) as a suitable strategy by electrophoretic deposition (EPD).
31500015	3	37	theme	sphere	614:619	arg1	size					621:624	uniform sphere size	606:624	uniform sphere size (~100 nm)	606:634	In this study, we prepared mesoporous silica nanoparticles (MSN) and MSN-COOH with uniform sphere size (~100 nm) and developed multifunctional chitosan coatings loaded with MSN@GHK-Cu (glycyl-L-histidyl-l-lysine-Cu2+) as a suitable strategy by electrophoretic deposition (EPD).
31500015	5	38	theme	surface	1039:1045	arg1	value					1017:1021	the pH value	1010:1021	the pH value of the titanium surface	1010:1045	The functional activity of Cu2+ releasing from the surface was dependent on the pH value of the titanium surface.
31500015	2	39	theme	Concentration-dependent	348:370	arg1	effects					372:378	Concentration-dependent effects	348:378	Concentration-dependent effects of therapeutic copper ions (Cu2+) for antibacterial and osteogenic activity	348:454	Concentration-dependent effects of therapeutic copper ions (Cu2+) for antibacterial and osteogenic activity have been well-established in the field of biomedical application.
31500015	5	40	theme	titanium	1030:1037	arg1	surface					1039:1045	the titanium surface	1026:1045	the titanium surface	1026:1045	The functional activity of Cu2+ releasing from the surface was dependent on the pH value of the titanium surface.
31500015	4	41	theme	coating	843:849	arg1	composition					824:834	composition	824:834	composition	824:834	The microstructure and composition of the coating were comprehensively characterized by using SEM, XRD, FTIR, and TEM, respectively.
31500015	4	41	theme	coating	843:849	arg1	microstructure					805:818	microstructure	805:818	microstructure	805:818	The microstructure and composition of the coating were comprehensively characterized by using SEM, XRD, FTIR, and TEM, respectively.
31500015	7	42	theme	modification	1266:1277	arg1	coating					1192:1198	The coating	1188:1198	The coating based on EPD technique	1188:1221	The coating based on EPD technique could be considered as a promising surface modification approach for the controlled delivery in situ of drug or other biomolecules.
31500015	7	42	theme	modification	1266:1277	arg1	approach					1279:1286	a promising surface modification approach	1246:1286	a promising surface modification approach for the controlled delivery in situ of drug or other biomolecules	1246:1352	The coating based on EPD technique could be considered as a promising surface modification approach for the controlled delivery in situ of drug or other biomolecules.
31500015	3	43	theme	MSN	696:698	arg1	glycyl-L-histidyl-l-lysine-Cu2+					708:738	glycyl-L-histidyl-l-lysine-Cu2+	708:738	glycyl-L-histidyl-l-lysine-Cu2+	708:738	In this study, we prepared mesoporous silica nanoparticles (MSN) and MSN-COOH with uniform sphere size (~100 nm) and developed multifunctional chitosan coatings loaded with MSN@GHK-Cu (glycyl-L-histidyl-l-lysine-Cu2+) as a suitable strategy by electrophoretic deposition (EPD).
31500015	3	43	theme	MSN	696:698	arg1	GHK-Cu					700:705	MSN@GHK-Cu	696:705	MSN@GHK-Cu (glycyl-L-histidyl-l-lysine-Cu2+)	696:739	In this study, we prepared mesoporous silica nanoparticles (MSN) and MSN-COOH with uniform sphere size (~100 nm) and developed multifunctional chitosan coatings loaded with MSN@GHK-Cu (glycyl-L-histidyl-l-lysine-Cu2+) as a suitable strategy by electrophoretic deposition (EPD).
31500015	7	44	theme	biomolecules	1341:1352	arg1	delivery					1307:1314	the controlled delivery	1292:1314	the controlled delivery in situ of drug or other biomolecules	1292:1352	The coating based on EPD technique could be considered as a promising surface modification approach for the controlled delivery in situ of drug or other biomolecules.
31500015	7	45	from	delivery	1307:1314	arg1	situ					1319:1322	situ	1319:1322	situ	1319:1322	The coating based on EPD technique could be considered as a promising surface modification approach for the controlled delivery in situ of drug or other biomolecules.
31500015	7	46	theme	promising	1248:1256	arg1	coating					1192:1198	The coating	1188:1198	The coating based on EPD technique	1188:1221	The coating based on EPD technique could be considered as a promising surface modification approach for the controlled delivery in situ of drug or other biomolecules.
31500015	7	46	theme	promising	1248:1256	arg1	approach					1279:1286	a promising surface modification approach	1246:1286	a promising surface modification approach for the controlled delivery in situ of drug or other biomolecules	1246:1352	The coating based on EPD technique could be considered as a promising surface modification approach for the controlled delivery in situ of drug or other biomolecules.
31500015	1	47	theme	prosthesis	215:224	arg1	failure					226:232	prosthesis failure	215:232	prosthesis failure	215:232	Despite the fact that titanium has been widely applied in the replacement of bone defects, prosthesis failure still occurred because of the lack of adequate bone-bonding ability and the incidence of post-surgery infections.
31500015	5	48	theme	releasing	966:974	arg1	Cu2+					961:964	Cu2+ releasing	961:974	Cu2+ releasing	961:974	The functional activity of Cu2+ releasing from the surface was dependent on the pH value of the titanium surface.
31500015	7	49	theme	surface	1258:1264	arg1	coating					1192:1198	The coating	1188:1198	The coating based on EPD technique	1188:1221	The coating based on EPD technique could be considered as a promising surface modification approach for the controlled delivery in situ of drug or other biomolecules.
31500015	7	49	theme	surface	1258:1264	arg1	approach					1279:1286	a promising surface modification approach	1246:1286	a promising surface modification approach for the controlled delivery in situ of drug or other biomolecules	1246:1352	The coating based on EPD technique could be considered as a promising surface modification approach for the controlled delivery in situ of drug or other biomolecules.
31500015	6	50	contain	had	1160:1162	arg2	cytocompatibility					1169:1185	good cytocompatibility	1164:1185	good cytocompatibility	1164:1185	Through the controllable release of Cu2+, the coating achieved not only inhibited adhesion of bacteria but also had good cytocompatibility.
31500015	6	50	contain	had	1160:1162	arg1	coating					1094:1100	the coating	1090:1100	the coating	1090:1100	Through the controllable release of Cu2+, the coating achieved not only inhibited adhesion of bacteria but also had good cytocompatibility.
31500015	7	51	theme	controlled	1296:1305	arg1	delivery					1307:1314	the controlled delivery	1292:1314	the controlled delivery in situ of drug or other biomolecules	1292:1352	The coating based on EPD technique could be considered as a promising surface modification approach for the controlled delivery in situ of drug or other biomolecules.
31500015	2	52	theme	copper	395:400	arg1	Cu2+					408:411	Cu2+	408:411	Cu2+	408:411	Concentration-dependent effects of therapeutic copper ions (Cu2+) for antibacterial and osteogenic activity have been well-established in the field of biomedical application.
31500015	2	52	theme	copper	395:400	arg1	ions					402:405	therapeutic copper ions	383:405	therapeutic copper ions (Cu2+)	383:412	Concentration-dependent effects of therapeutic copper ions (Cu2+) for antibacterial and osteogenic activity have been well-established in the field of biomedical application.
31500015	0	53	theme	pH-responsive	71:83	arg1	release					85:91	pH-responsive release	71:91	pH-responsive release of copper and its bioactivity	71:121	Electrophoretic deposition of GHK-Cu loaded MSN-chitosan coatings with pH-responsive release of copper and its bioactivity.
31500015	1	54	theme	post-surgery	323:334	arg1	infections					336:345	post-surgery infections	323:345	post-surgery infections	323:345	Despite the fact that titanium has been widely applied in the replacement of bone defects, prosthesis failure still occurred because of the lack of adequate bone-bonding ability and the incidence of post-surgery infections.
31500015	7	55	theme	other	1335:1339	arg1	biomolecules					1341:1352	other biomolecules	1335:1352	other biomolecules	1335:1352	The coating based on EPD technique could be considered as a promising surface modification approach for the controlled delivery in situ of drug or other biomolecules.
31500015	2	56	theme	therapeutic	383:393	arg1	Cu2+					408:411	Cu2+	408:411	Cu2+	408:411	Concentration-dependent effects of therapeutic copper ions (Cu2+) for antibacterial and osteogenic activity have been well-established in the field of biomedical application.
31500015	2	56	theme	therapeutic	383:393	arg1	ions					402:405	therapeutic copper ions	383:405	therapeutic copper ions (Cu2+)	383:412	Concentration-dependent effects of therapeutic copper ions (Cu2+) for antibacterial and osteogenic activity have been well-established in the field of biomedical application.
30142524	7	0	theme	Cur-loaded	1430:1439	arg1	films					1451:1455	the Cur-loaded lipid@CNF films	1426:1455	the Cur-loaded lipid@CNF films	1426:1455	The in vivo anti-psoriatic efficacy test revealed that the Cur-loaded lipid@CNF films ameliorated the psoriatic skin symptoms in IMQ-induced mice, reducing the pro-inflammatory cytokine levels in the skin almost comparable to a commercially available topical corticosteroid cream.
30142524	6	1	theme	lipid	1054:1058	arg1	films					1064:1068	The Cur-loaded lipid@CNF films	1039:1068	The Cur-loaded lipid@CNF films	1039:1068	The Cur-loaded lipid@CNF films exhibited more than 2.0-fold increases in Cur deposition to the epidermis of imiquimod (IMQ)-induced psoriatic mouse compared with the films without lipids, which potentially resulted from the amorphous state of Cur observed in the DSC and PXRD analyses and the permeation-enhancing effect of lipids.
30142524	4	2	contain	containing	675:684	arg2	EP					713:714	shea butter and Capmul MCM EP	686:714	EP	713:714	The lipid composition of the NLCs was optimized based on the efficiency of Cur delivery to the artificial skin and mechanical strength of the developed films, where a composition containing shea butter and Capmul MCM EP exhibited the highest values of 233.2 ± 96.6 μg/cm2/mg and 4.86 ± 0.14 MPa, respectively.
30142524	4	2	contain	containing	675:684	arg2	butter					691:696	shea butter and Capmul MCM EP	686:714	butter	691:696	The lipid composition of the NLCs was optimized based on the efficiency of Cur delivery to the artificial skin and mechanical strength of the developed films, where a composition containing shea butter and Capmul MCM EP exhibited the highest values of 233.2 ± 96.6 μg/cm2/mg and 4.86 ± 0.14 MPa, respectively.
30142524	4	2	contain	containing	675:684	arg1	composition					663:673	a composition	661:673	a composition containing shea butter and Capmul MCM EP	661:714	The lipid composition of the NLCs was optimized based on the efficiency of Cur delivery to the artificial skin and mechanical strength of the developed films, where a composition containing shea butter and Capmul MCM EP exhibited the highest values of 233.2 ± 96.6 μg/cm2/mg and 4.86 ± 0.14 MPa, respectively.
30142524	2	3	theme	lipid	383:387	arg1	carriers					389:396	nanostructured lipid carriers	368:396	curcumin (Cur)-loaded nanostructured lipid carriers (NLCs)	346:403	Herein, CNF film was prepared as a topical drug delivery system by hybridizing curcumin (Cur)-loaded nanostructured lipid carriers (NLCs).
30142524	2	3	theme	lipid	383:387	arg1	NLCs					399:402	NLCs	399:402	NLCs	399:402	Herein, CNF film was prepared as a topical drug delivery system by hybridizing curcumin (Cur)-loaded nanostructured lipid carriers (NLCs).
30142524	4	4	theme	artificial	591:600	arg1	skin					602:605	artificial skin	591:605	artificial skin	591:605	The lipid composition of the NLCs was optimized based on the efficiency of Cur delivery to the artificial skin and mechanical strength of the developed films, where a composition containing shea butter and Capmul MCM EP exhibited the highest values of 233.2 ± 96.6 μg/cm2/mg and 4.86 ± 0.14 MPa, respectively.
30142524	2	5	theme	delivery	315:322	arg1	system					324:329	a topical drug delivery system	300:329	a topical drug delivery system	300:329	Herein, CNF film was prepared as a topical drug delivery system by hybridizing curcumin (Cur)-loaded nanostructured lipid carriers (NLCs).
30142524	2	5	theme	delivery	315:322	arg1	film					279:282	CNF film	275:282	CNF film	275:282	Herein, CNF film was prepared as a topical drug delivery system by hybridizing curcumin (Cur)-loaded nanostructured lipid carriers (NLCs).
30142524	9	6	theme	topical	1847:1853	arg1	films					1814:1818	the developed CNF films	1796:1818	the developed CNF films	1796:1818	These findings suggest that the developed CNF films can be used as a promising topical drug delivery system for psoriasis therapy.
30142524	9	6	theme	topical	1847:1853	arg1	system					1869:1874	a promising topical drug delivery system	1835:1874	a promising topical drug delivery system for psoriasis therapy	1835:1896	These findings suggest that the developed CNF films can be used as a promising topical drug delivery system for psoriasis therapy.
30142524	2	7	theme	topical	302:308	arg1	system					324:329	a topical drug delivery system	300:329	a topical drug delivery system	300:329	Herein, CNF film was prepared as a topical drug delivery system by hybridizing curcumin (Cur)-loaded nanostructured lipid carriers (NLCs).
30142524	2	7	theme	topical	302:308	arg1	film					279:282	CNF film	275:282	CNF film	275:282	Herein, CNF film was prepared as a topical drug delivery system by hybridizing curcumin (Cur)-loaded nanostructured lipid carriers (NLCs).
30142524	7	8	theme	anti-psoriatic	1383:1396	arg1	test					1407:1410	The in vivo anti-psoriatic efficacy test	1371:1410	The in vivo anti-psoriatic efficacy test	1371:1410	The in vivo anti-psoriatic efficacy test revealed that the Cur-loaded lipid@CNF films ameliorated the psoriatic skin symptoms in IMQ-induced mice, reducing the pro-inflammatory cytokine levels in the skin almost comparable to a commercially available topical corticosteroid cream.
30142524	6	9	theme	Cur	1112:1114	arg1	deposition					1116:1125	Cur deposition	1112:1125	Cur deposition to the epidermis of imiquimod (IMQ)-induced psoriatic mouse	1112:1185	The Cur-loaded lipid@CNF films exhibited more than 2.0-fold increases in Cur deposition to the epidermis of imiquimod (IMQ)-induced psoriatic mouse compared with the films without lipids, which potentially resulted from the amorphous state of Cur observed in the DSC and PXRD analyses and the permeation-enhancing effect of lipids.
30142524	9	10	theme	delivery	1860:1867	arg1	films					1814:1818	the developed CNF films	1796:1818	the developed CNF films	1796:1818	These findings suggest that the developed CNF films can be used as a promising topical drug delivery system for psoriasis therapy.
30142524	9	10	theme	delivery	1860:1867	arg1	system					1869:1874	a promising topical drug delivery system	1835:1874	a promising topical drug delivery system for psoriasis therapy	1835:1896	These findings suggest that the developed CNF films can be used as a promising topical drug delivery system for psoriasis therapy.
30142524	1	11	theme	mechanical	223:232	arg1	properties					234:243	excellent mechanical properties	213:243	excellent mechanical properties	213:243	Cellulose nanofiber (CNF) is an attractive biomaterial given its film-forming properties, excellent mechanical properties and biocompatibility.
30142524	3	12	with	NLCs	406:409	arg1	diameter					423:430	a mean diameter	416:430	a mean diameter of ≈500 nm	416:441	NLCs with a mean diameter of ≈500 nm were fabricated by using a solvent diffusion method.
30142524	5	13	theme	FTIR	1024:1027	arg1	analyses					1029:1036	FTIR analyses	1024:1036	FTIR analyses	1024:1036	The Cur-loaded lipid-hybridized CNF (lipid@CNF) films with a smooth rather than particle-embedded surface were obtained by vacuum filtration of the NLCs and CNF mixture, which were confirmed by TEM, SEM, AFM, XPS, and FTIR analyses.
30142524	8	14	theme	Cur	1702:1704	arg1	deposition					1706:1715	the enhanced Cur deposition	1689:1715	the enhanced Cur deposition along with the skin hydration effect of the films	1689:1765	These results could be attributed to the enhanced Cur deposition along with the skin hydration effect of the films.
30142524	6	15	theme	mouse	1181:1185	arg1	epidermis					1134:1142	the epidermis	1130:1142	the epidermis of imiquimod (IMQ)-induced psoriatic mouse	1130:1185	The Cur-loaded lipid@CNF films exhibited more than 2.0-fold increases in Cur deposition to the epidermis of imiquimod (IMQ)-induced psoriatic mouse compared with the films without lipids, which potentially resulted from the amorphous state of Cur observed in the DSC and PXRD analyses and the permeation-enhancing effect of lipids.
30142524	4	16	theme	films	648:652	arg1	skin					602:605	artificial skin	591:605	artificial skin	591:605	The lipid composition of the NLCs was optimized based on the efficiency of Cur delivery to the artificial skin and mechanical strength of the developed films, where a composition containing shea butter and Capmul MCM EP exhibited the highest values of 233.2 ± 96.6 μg/cm2/mg and 4.86 ± 0.14 MPa, respectively.
30142524	4	16	theme	films	648:652	arg1	strength					622:629	mechanical strength	611:629	mechanical strength	611:629	The lipid composition of the NLCs was optimized based on the efficiency of Cur delivery to the artificial skin and mechanical strength of the developed films, where a composition containing shea butter and Capmul MCM EP exhibited the highest values of 233.2 ± 96.6 μg/cm2/mg and 4.86 ± 0.14 MPa, respectively.
30142524	6	17	theme	amorphous	1263:1271	arg1	state					1273:1277	the amorphous state	1259:1277	the amorphous state of Cur observed in the DSC and PXRD analyses	1259:1322	The Cur-loaded lipid@CNF films exhibited more than 2.0-fold increases in Cur deposition to the epidermis of imiquimod (IMQ)-induced psoriatic mouse compared with the films without lipids, which potentially resulted from the amorphous state of Cur observed in the DSC and PXRD analyses and the permeation-enhancing effect of lipids.
30142524	2	18	theme	CNF	275:277	arg1	system					324:329	a topical drug delivery system	300:329	a topical drug delivery system	300:329	Herein, CNF film was prepared as a topical drug delivery system by hybridizing curcumin (Cur)-loaded nanostructured lipid carriers (NLCs).
30142524	2	18	theme	CNF	275:277	arg1	film					279:282	CNF film	275:282	CNF film	275:282	Herein, CNF film was prepared as a topical drug delivery system by hybridizing curcumin (Cur)-loaded nanostructured lipid carriers (NLCs).
30142524	0	19	from	dermatitis	103:112	arg1	mice					117:120	mice	117:120	mice	117:120	Curcumin-loaded lipid-hybridized cellulose nanofiber film ameliorates imiquimod-induced psoriasis-like dermatitis in mice.
30142524	6	20	theme	-induced	1162:1169	arg1	mouse					1181:1185	imiquimod (IMQ)-induced psoriatic mouse	1147:1185	imiquimod (IMQ)-induced psoriatic mouse	1147:1185	The Cur-loaded lipid@CNF films exhibited more than 2.0-fold increases in Cur deposition to the epidermis of imiquimod (IMQ)-induced psoriatic mouse compared with the films without lipids, which potentially resulted from the amorphous state of Cur observed in the DSC and PXRD analyses and the permeation-enhancing effect of lipids.
30142524	4	21	theme	MCM	709:711	arg1	EP					713:714	shea butter and Capmul MCM EP	686:714	EP	713:714	The lipid composition of the NLCs was optimized based on the efficiency of Cur delivery to the artificial skin and mechanical strength of the developed films, where a composition containing shea butter and Capmul MCM EP exhibited the highest values of 233.2 ± 96.6 μg/cm2/mg and 4.86 ± 0.14 MPa, respectively.
30142524	8	22	theme	hydration	1737:1745	arg1	effect					1747:1752	the skin hydration effect	1728:1752	the skin hydration effect of the films	1728:1765	These results could be attributed to the enhanced Cur deposition along with the skin hydration effect of the films.
30142524	1	23	theme	attractive	155:164	arg1	nanofiber					133:141	Cellulose nanofiber	123:141	Cellulose nanofiber (CNF)	123:147	Cellulose nanofiber (CNF) is an attractive biomaterial given its film-forming properties, excellent mechanical properties and biocompatibility.
30142524	1	23	theme	attractive	155:164	arg1	biomaterial					166:176	an attractive biomaterial	152:176	an attractive biomaterial given its film-forming properties, excellent mechanical properties and biocompatibility	152:264	Cellulose nanofiber (CNF) is an attractive biomaterial given its film-forming properties, excellent mechanical properties and biocompatibility.
30142524	7	24	theme	pro-inflammatory	1531:1546	arg1	levels					1557:1562	the pro-inflammatory cytokine levels	1527:1562	the pro-inflammatory cytokine levels in the skin almost comparable to a commercially available topical corticosteroid cream	1527:1649	The in vivo anti-psoriatic efficacy test revealed that the Cur-loaded lipid@CNF films ameliorated the psoriatic skin symptoms in IMQ-induced mice, reducing the pro-inflammatory cytokine levels in the skin almost comparable to a commercially available topical corticosteroid cream.
30142524	5	25	dep	NLCs	954:957	arg1	the					950:952	the	950:952	the	950:952	The Cur-loaded lipid-hybridized CNF (lipid@CNF) films with a smooth rather than particle-embedded surface were obtained by vacuum filtration of the NLCs and CNF mixture, which were confirmed by TEM, SEM, AFM, XPS, and FTIR analyses.
30142524	6	26	theme	lipids	1363:1368	arg1	effect					1353:1358	the permeation-enhancing effect	1328:1358	the permeation-enhancing effect of lipids	1328:1368	The Cur-loaded lipid@CNF films exhibited more than 2.0-fold increases in Cur deposition to the epidermis of imiquimod (IMQ)-induced psoriatic mouse compared with the films without lipids, which potentially resulted from the amorphous state of Cur observed in the DSC and PXRD analyses and the permeation-enhancing effect of lipids.
30142524	6	26	theme	lipids	1363:1368	arg1	state					1273:1277	the amorphous state	1259:1277	the amorphous state of Cur observed in the DSC and PXRD analyses	1259:1322	The Cur-loaded lipid@CNF films exhibited more than 2.0-fold increases in Cur deposition to the epidermis of imiquimod (IMQ)-induced psoriatic mouse compared with the films without lipids, which potentially resulted from the amorphous state of Cur observed in the DSC and PXRD analyses and the permeation-enhancing effect of lipids.
30142524	5	27	theme	particle-embedded	886:902	arg1	surface					904:910	a smooth rather than particle-embedded surface	865:910	a smooth rather than particle-embedded surface	865:910	The Cur-loaded lipid-hybridized CNF (lipid@CNF) films with a smooth rather than particle-embedded surface were obtained by vacuum filtration of the NLCs and CNF mixture, which were confirmed by TEM, SEM, AFM, XPS, and FTIR analyses.
30142524	7	28	from	levels	1557:1562	arg1	skin					1571:1574	the skin	1567:1574	the skin almost comparable to a commercially available topical corticosteroid cream	1567:1649	The in vivo anti-psoriatic efficacy test revealed that the Cur-loaded lipid@CNF films ameliorated the psoriatic skin symptoms in IMQ-induced mice, reducing the pro-inflammatory cytokine levels in the skin almost comparable to a commercially available topical corticosteroid cream.
30142524	8	29	theme	films	1761:1765	arg1	effect					1747:1752	the skin hydration effect	1728:1752	the skin hydration effect of the films	1728:1765	These results could be attributed to the enhanced Cur deposition along with the skin hydration effect of the films.
30142524	1	30	theme	film-forming	188:199	arg1	properties					201:210	its film-forming properties	184:210	its film-forming properties	184:210	Cellulose nanofiber (CNF) is an attractive biomaterial given its film-forming properties, excellent mechanical properties and biocompatibility.
30142524	5	31	theme	mixture	967:973	arg1	filtration					936:945	vacuum filtration	929:945	vacuum filtration of the NLCs and CNF mixture, which were confirmed by TEM, SEM, AFM, XPS, and FTIR analyses	929:1036	The Cur-loaded lipid-hybridized CNF (lipid@CNF) films with a smooth rather than particle-embedded surface were obtained by vacuum filtration of the NLCs and CNF mixture, which were confirmed by TEM, SEM, AFM, XPS, and FTIR analyses.
30142524	7	32	dep	in	1375:1376	arg1	vivo					1378:1381	vivo	1378:1381	vivo	1378:1381	The in vivo anti-psoriatic efficacy test revealed that the Cur-loaded lipid@CNF films ameliorated the psoriatic skin symptoms in IMQ-induced mice, reducing the pro-inflammatory cytokine levels in the skin almost comparable to a commercially available topical corticosteroid cream.
30142524	7	33	theme	corticosteroid	1630:1643	arg1	cream					1645:1649	a commercially available topical corticosteroid cream	1597:1649	a commercially available topical corticosteroid cream	1597:1649	The in vivo anti-psoriatic efficacy test revealed that the Cur-loaded lipid@CNF films ameliorated the psoriatic skin symptoms in IMQ-induced mice, reducing the pro-inflammatory cytokine levels in the skin almost comparable to a commercially available topical corticosteroid cream.
30142524	4	34	theme	highest	730:736	arg1	values					738:743	the highest values	726:743	the highest values of 233.2 ± 96.6 μg/cm2/mg and 4.86 ± 0.14 MPa	726:789	The lipid composition of the NLCs was optimized based on the efficiency of Cur delivery to the artificial skin and mechanical strength of the developed films, where a composition containing shea butter and Capmul MCM EP exhibited the highest values of 233.2 ± 96.6 μg/cm2/mg and 4.86 ± 0.14 MPa, respectively.
30142524	3	35	theme	≈500 nm	435:441	arg1	diameter					423:430	a mean diameter	416:430	a mean diameter of ≈500 nm	416:441	NLCs with a mean diameter of ≈500 nm were fabricated by using a solvent diffusion method.
30142524	0	36	theme	Curcumin-loaded	0:14	arg1	film					53:56	Curcumin-loaded lipid-hybridized cellulose nanofiber film	0:56	Curcumin-loaded lipid-hybridized cellulose nanofiber film	0:56	Curcumin-loaded lipid-hybridized cellulose nanofiber film ameliorates imiquimod-induced psoriasis-like dermatitis in mice.
30142524	7	37	dep	mice	1512:1515	arg1	reducing					1518:1525	reducing	1518:1525	reducing the pro-inflammatory cytokine levels in the skin almost comparable to a commercially available topical corticosteroid cream	1518:1649	The in vivo anti-psoriatic efficacy test revealed that the Cur-loaded lipid@CNF films ameliorated the psoriatic skin symptoms in IMQ-induced mice, reducing the pro-inflammatory cytokine levels in the skin almost comparable to a commercially available topical corticosteroid cream.
30142524	7	38	theme	available	1612:1620	arg1	cream					1645:1649	a commercially available topical corticosteroid cream	1597:1649	a commercially available topical corticosteroid cream	1597:1649	The in vivo anti-psoriatic efficacy test revealed that the Cur-loaded lipid@CNF films ameliorated the psoriatic skin symptoms in IMQ-induced mice, reducing the pro-inflammatory cytokine levels in the skin almost comparable to a commercially available topical corticosteroid cream.
30142524	3	39	theme	solvent	470:476	arg1	method					488:493	a solvent diffusion method	468:493	a solvent diffusion method	468:493	NLCs with a mean diameter of ≈500 nm were fabricated by using a solvent diffusion method.
30142524	0	40	theme	cellulose	33:41	arg1	film					53:56	Curcumin-loaded lipid-hybridized cellulose nanofiber film	0:56	Curcumin-loaded lipid-hybridized cellulose nanofiber film	0:56	Curcumin-loaded lipid-hybridized cellulose nanofiber film ameliorates imiquimod-induced psoriasis-like dermatitis in mice.
30142524	7	41	theme	psoriatic	1473:1481	arg1	symptoms					1488:1495	the psoriatic skin symptoms	1469:1495	the psoriatic skin symptoms in IMQ-induced mice, reducing the pro-inflammatory cytokine levels in the skin almost comparable to a commercially available topical corticosteroid cream	1469:1649	The in vivo anti-psoriatic efficacy test revealed that the Cur-loaded lipid@CNF films ameliorated the psoriatic skin symptoms in IMQ-induced mice, reducing the pro-inflammatory cytokine levels in the skin almost comparable to a commercially available topical corticosteroid cream.
30142524	4	42	theme	shea	686:689	arg1	butter					691:696	shea butter and Capmul MCM EP	686:714	butter	691:696	The lipid composition of the NLCs was optimized based on the efficiency of Cur delivery to the artificial skin and mechanical strength of the developed films, where a composition containing shea butter and Capmul MCM EP exhibited the highest values of 233.2 ± 96.6 μg/cm2/mg and 4.86 ± 0.14 MPa, respectively.
30142524	2	43	theme	nanostructured	368:381	arg1	carriers					389:396	nanostructured lipid carriers	368:396	curcumin (Cur)-loaded nanostructured lipid carriers (NLCs)	346:403	Herein, CNF film was prepared as a topical drug delivery system by hybridizing curcumin (Cur)-loaded nanostructured lipid carriers (NLCs).
30142524	2	43	theme	nanostructured	368:381	arg1	NLCs					399:402	NLCs	399:402	NLCs	399:402	Herein, CNF film was prepared as a topical drug delivery system by hybridizing curcumin (Cur)-loaded nanostructured lipid carriers (NLCs).
30142524	5	44	theme	Cur-loaded	810:819	arg1	CNF					838:840	Cur-loaded lipid-hybridized CNF	810:840	The Cur-loaded lipid-hybridized CNF (lipid@CNF) films with a smooth rather than particle-embedded surface	806:910	The Cur-loaded lipid-hybridized CNF (lipid@CNF) films with a smooth rather than particle-embedded surface were obtained by vacuum filtration of the NLCs and CNF mixture, which were confirmed by TEM, SEM, AFM, XPS, and FTIR analyses.
30142524	5	44	theme	Cur-loaded	810:819	arg1	CNF					849:851	lipid@CNF	843:851	lipid@CNF	843:851	The Cur-loaded lipid-hybridized CNF (lipid@CNF) films with a smooth rather than particle-embedded surface were obtained by vacuum filtration of the NLCs and CNF mixture, which were confirmed by TEM, SEM, AFM, XPS, and FTIR analyses.
30142524	7	45	theme	CNF	1447:1449	arg1	films					1451:1455	the Cur-loaded lipid@CNF films	1426:1455	the Cur-loaded lipid@CNF films	1426:1455	The in vivo anti-psoriatic efficacy test revealed that the Cur-loaded lipid@CNF films ameliorated the psoriatic skin symptoms in IMQ-induced mice, reducing the pro-inflammatory cytokine levels in the skin almost comparable to a commercially available topical corticosteroid cream.
30142524	9	46	theme	developed	1800:1808	arg1	films					1814:1818	the developed CNF films	1796:1818	the developed CNF films	1796:1818	These findings suggest that the developed CNF films can be used as a promising topical drug delivery system for psoriasis therapy.
30142524	9	46	theme	developed	1800:1808	arg1	system					1869:1874	a promising topical drug delivery system	1835:1874	a promising topical drug delivery system for psoriasis therapy	1835:1896	These findings suggest that the developed CNF films can be used as a promising topical drug delivery system for psoriasis therapy.
30142524	7	47	theme	lipid	1441:1445	arg1	films					1451:1455	the Cur-loaded lipid@CNF films	1426:1455	the Cur-loaded lipid@CNF films	1426:1455	The in vivo anti-psoriatic efficacy test revealed that the Cur-loaded lipid@CNF films ameliorated the psoriatic skin symptoms in IMQ-induced mice, reducing the pro-inflammatory cytokine levels in the skin almost comparable to a commercially available topical corticosteroid cream.
30142524	5	48	theme	lipid	843:847	arg1	CNF					838:840	Cur-loaded lipid-hybridized CNF	810:840	The Cur-loaded lipid-hybridized CNF (lipid@CNF) films with a smooth rather than particle-embedded surface	806:910	The Cur-loaded lipid-hybridized CNF (lipid@CNF) films with a smooth rather than particle-embedded surface were obtained by vacuum filtration of the NLCs and CNF mixture, which were confirmed by TEM, SEM, AFM, XPS, and FTIR analyses.
30142524	5	48	theme	lipid	843:847	arg1	CNF					849:851	lipid@CNF	843:851	lipid@CNF	843:851	The Cur-loaded lipid-hybridized CNF (lipid@CNF) films with a smooth rather than particle-embedded surface were obtained by vacuum filtration of the NLCs and CNF mixture, which were confirmed by TEM, SEM, AFM, XPS, and FTIR analyses.
30142524	6	49	theme	permeation-enhancing	1332:1351	arg1	effect					1353:1358	the permeation-enhancing effect	1328:1358	the permeation-enhancing effect of lipids	1328:1368	The Cur-loaded lipid@CNF films exhibited more than 2.0-fold increases in Cur deposition to the epidermis of imiquimod (IMQ)-induced psoriatic mouse compared with the films without lipids, which potentially resulted from the amorphous state of Cur observed in the DSC and PXRD analyses and the permeation-enhancing effect of lipids.
30142524	6	50	theme	@	1059:1059	arg1	films					1064:1068	The Cur-loaded lipid@CNF films	1039:1068	The Cur-loaded lipid@CNF films	1039:1068	The Cur-loaded lipid@CNF films exhibited more than 2.0-fold increases in Cur deposition to the epidermis of imiquimod (IMQ)-induced psoriatic mouse compared with the films without lipids, which potentially resulted from the amorphous state of Cur observed in the DSC and PXRD analyses and the permeation-enhancing effect of lipids.
30142524	4	51	theme	mechanical	611:620	arg1	strength					622:629	mechanical strength	611:629	mechanical strength	611:629	The lipid composition of the NLCs was optimized based on the efficiency of Cur delivery to the artificial skin and mechanical strength of the developed films, where a composition containing shea butter and Capmul MCM EP exhibited the highest values of 233.2 ± 96.6 μg/cm2/mg and 4.86 ± 0.14 MPa, respectively.
30142524	6	52	theme	Cur-loaded	1043:1052	arg1	films					1064:1068	The Cur-loaded lipid@CNF films	1039:1068	The Cur-loaded lipid@CNF films	1039:1068	The Cur-loaded lipid@CNF films exhibited more than 2.0-fold increases in Cur deposition to the epidermis of imiquimod (IMQ)-induced psoriatic mouse compared with the films without lipids, which potentially resulted from the amorphous state of Cur observed in the DSC and PXRD analyses and the permeation-enhancing effect of lipids.
30142524	4	53	theme	delivery	575:582	arg1	efficiency					557:566	the efficiency	553:566	the efficiency of Cur delivery to the artificial skin and mechanical strength of the developed films, where a composition containing shea butter and Capmul MCM EP exhibited the highest values of 233.2 ± 96.6 μg/cm2/mg and 4.86 ± 0.14 MPa, respectively	553:803	The lipid composition of the NLCs was optimized based on the efficiency of Cur delivery to the artificial skin and mechanical strength of the developed films, where a composition containing shea butter and Capmul MCM EP exhibited the highest values of 233.2 ± 96.6 μg/cm2/mg and 4.86 ± 0.14 MPa, respectively.
30142524	7	54	theme	efficacy	1398:1405	arg1	test					1407:1410	The in vivo anti-psoriatic efficacy test	1371:1410	The in vivo anti-psoriatic efficacy test	1371:1410	The in vivo anti-psoriatic efficacy test revealed that the Cur-loaded lipid@CNF films ameliorated the psoriatic skin symptoms in IMQ-induced mice, reducing the pro-inflammatory cytokine levels in the skin almost comparable to a commercially available topical corticosteroid cream.
30142524	6	55	located	observed	1286:1293	arg2	Cur					1282:1284	Cur	1282:1284	Cur observed in the DSC and PXRD analyses	1282:1322	The Cur-loaded lipid@CNF films exhibited more than 2.0-fold increases in Cur deposition to the epidermis of imiquimod (IMQ)-induced psoriatic mouse compared with the films without lipids, which potentially resulted from the amorphous state of Cur observed in the DSC and PXRD analyses and the permeation-enhancing effect of lipids.
30142524	6	55	located	observed	1286:1293	arg1	analyses					1315:1322	the DSC and PXRD analyses	1298:1322	analyses	1315:1322	The Cur-loaded lipid@CNF films exhibited more than 2.0-fold increases in Cur deposition to the epidermis of imiquimod (IMQ)-induced psoriatic mouse compared with the films without lipids, which potentially resulted from the amorphous state of Cur observed in the DSC and PXRD analyses and the permeation-enhancing effect of lipids.
30142524	7	56	theme	in	1375:1376	arg1	test					1407:1410	The in vivo anti-psoriatic efficacy test	1371:1410	The in vivo anti-psoriatic efficacy test	1371:1410	The in vivo anti-psoriatic efficacy test revealed that the Cur-loaded lipid@CNF films ameliorated the psoriatic skin symptoms in IMQ-induced mice, reducing the pro-inflammatory cytokine levels in the skin almost comparable to a commercially available topical corticosteroid cream.
30142524	9	57	theme	promising	1837:1845	arg1	films					1814:1818	the developed CNF films	1796:1818	the developed CNF films	1796:1818	These findings suggest that the developed CNF films can be used as a promising topical drug delivery system for psoriasis therapy.
30142524	9	57	theme	promising	1837:1845	arg1	system					1869:1874	a promising topical drug delivery system	1835:1874	a promising topical drug delivery system for psoriasis therapy	1835:1896	These findings suggest that the developed CNF films can be used as a promising topical drug delivery system for psoriasis therapy.
30142524	2	58	theme	drug	310:313	arg1	system					324:329	a topical drug delivery system	300:329	a topical drug delivery system	300:329	Herein, CNF film was prepared as a topical drug delivery system by hybridizing curcumin (Cur)-loaded nanostructured lipid carriers (NLCs).
30142524	2	58	theme	drug	310:313	arg1	film					279:282	CNF film	275:282	CNF film	275:282	Herein, CNF film was prepared as a topical drug delivery system by hybridizing curcumin (Cur)-loaded nanostructured lipid carriers (NLCs).
30142524	5	59	theme	smooth	867:872	arg1	surface					904:910	a smooth rather than particle-embedded surface	865:910	a smooth rather than particle-embedded surface	865:910	The Cur-loaded lipid-hybridized CNF (lipid@CNF) films with a smooth rather than particle-embedded surface were obtained by vacuum filtration of the NLCs and CNF mixture, which were confirmed by TEM, SEM, AFM, XPS, and FTIR analyses.
30142524	0	60	theme	imiquimod-induced	70:86	arg1	dermatitis					103:112	imiquimod-induced psoriasis-like dermatitis	70:112	imiquimod-induced psoriasis-like dermatitis in mice	70:120	Curcumin-loaded lipid-hybridized cellulose nanofiber film ameliorates imiquimod-induced psoriasis-like dermatitis in mice.
30142524	9	61	theme	drug	1855:1858	arg1	films					1814:1818	the developed CNF films	1796:1818	the developed CNF films	1796:1818	These findings suggest that the developed CNF films can be used as a promising topical drug delivery system for psoriasis therapy.
30142524	9	61	theme	drug	1855:1858	arg1	system					1869:1874	a promising topical drug delivery system	1835:1874	a promising topical drug delivery system for psoriasis therapy	1835:1896	These findings suggest that the developed CNF films can be used as a promising topical drug delivery system for psoriasis therapy.
30142524	1	62	theme	excellent	213:221	arg1	properties					234:243	excellent mechanical properties	213:243	excellent mechanical properties	213:243	Cellulose nanofiber (CNF) is an attractive biomaterial given its film-forming properties, excellent mechanical properties and biocompatibility.
30142524	7	63	theme	comparable	1583:1592	arg1	skin					1571:1574	the skin	1567:1574	the skin almost comparable to a commercially available topical corticosteroid cream	1567:1649	The in vivo anti-psoriatic efficacy test revealed that the Cur-loaded lipid@CNF films ameliorated the psoriatic skin symptoms in IMQ-induced mice, reducing the pro-inflammatory cytokine levels in the skin almost comparable to a commercially available topical corticosteroid cream.
30142524	6	64	theme	Cur	1282:1284	arg1	effect					1353:1358	the permeation-enhancing effect	1328:1358	the permeation-enhancing effect of lipids	1328:1368	The Cur-loaded lipid@CNF films exhibited more than 2.0-fold increases in Cur deposition to the epidermis of imiquimod (IMQ)-induced psoriatic mouse compared with the films without lipids, which potentially resulted from the amorphous state of Cur observed in the DSC and PXRD analyses and the permeation-enhancing effect of lipids.
30142524	6	64	theme	Cur	1282:1284	arg1	state					1273:1277	the amorphous state	1259:1277	the amorphous state of Cur observed in the DSC and PXRD analyses	1259:1322	The Cur-loaded lipid@CNF films exhibited more than 2.0-fold increases in Cur deposition to the epidermis of imiquimod (IMQ)-induced psoriatic mouse compared with the films without lipids, which potentially resulted from the amorphous state of Cur observed in the DSC and PXRD analyses and the permeation-enhancing effect of lipids.
30142524	5	65	theme	@	848:848	arg1	CNF					838:840	Cur-loaded lipid-hybridized CNF	810:840	The Cur-loaded lipid-hybridized CNF (lipid@CNF) films with a smooth rather than particle-embedded surface	806:910	The Cur-loaded lipid-hybridized CNF (lipid@CNF) films with a smooth rather than particle-embedded surface were obtained by vacuum filtration of the NLCs and CNF mixture, which were confirmed by TEM, SEM, AFM, XPS, and FTIR analyses.
30142524	5	65	theme	@	848:848	arg1	CNF					849:851	lipid@CNF	843:851	lipid@CNF	843:851	The Cur-loaded lipid-hybridized CNF (lipid@CNF) films with a smooth rather than particle-embedded surface were obtained by vacuum filtration of the NLCs and CNF mixture, which were confirmed by TEM, SEM, AFM, XPS, and FTIR analyses.
30142524	6	66	theme	2.0-fold	1090:1097	arg1	increases					1099:1107	2.0-fold increases	1090:1107	2.0-fold increases in Cur deposition to the epidermis of imiquimod (IMQ)-induced psoriatic mouse	1090:1185	The Cur-loaded lipid@CNF films exhibited more than 2.0-fold increases in Cur deposition to the epidermis of imiquimod (IMQ)-induced psoriatic mouse compared with the films without lipids, which potentially resulted from the amorphous state of Cur observed in the DSC and PXRD analyses and the permeation-enhancing effect of lipids.
30142524	9	67	theme	psoriasis	1880:1888	arg1	therapy					1890:1896	psoriasis therapy	1880:1896	psoriasis therapy	1880:1896	These findings suggest that the developed CNF films can be used as a promising topical drug delivery system for psoriasis therapy.
30142524	8	68	theme	enhanced	1693:1700	arg1	deposition					1706:1715	the enhanced Cur deposition	1689:1715	the enhanced Cur deposition along with the skin hydration effect of the films	1689:1765	These results could be attributed to the enhanced Cur deposition along with the skin hydration effect of the films.
30142524	6	69	theme	psoriatic	1171:1179	arg1	mouse					1181:1185	imiquimod (IMQ)-induced psoriatic mouse	1147:1185	imiquimod (IMQ)-induced psoriatic mouse	1147:1185	The Cur-loaded lipid@CNF films exhibited more than 2.0-fold increases in Cur deposition to the epidermis of imiquimod (IMQ)-induced psoriatic mouse compared with the films without lipids, which potentially resulted from the amorphous state of Cur observed in the DSC and PXRD analyses and the permeation-enhancing effect of lipids.
30142524	4	70	theme	developed	638:646	arg1	films					648:652	the developed films	634:652	the developed films	634:652	The lipid composition of the NLCs was optimized based on the efficiency of Cur delivery to the artificial skin and mechanical strength of the developed films, where a composition containing shea butter and Capmul MCM EP exhibited the highest values of 233.2 ± 96.6 μg/cm2/mg and 4.86 ± 0.14 MPa, respectively.
30142524	7	71	theme	cytokine	1548:1555	arg1	levels					1557:1562	the pro-inflammatory cytokine levels	1527:1562	the pro-inflammatory cytokine levels in the skin almost comparable to a commercially available topical corticosteroid cream	1527:1649	The in vivo anti-psoriatic efficacy test revealed that the Cur-loaded lipid@CNF films ameliorated the psoriatic skin symptoms in IMQ-induced mice, reducing the pro-inflammatory cytokine levels in the skin almost comparable to a commercially available topical corticosteroid cream.
30142524	4	72	theme	Capmul	702:707	arg1	EP					713:714	shea butter and Capmul MCM EP	686:714	EP	713:714	The lipid composition of the NLCs was optimized based on the efficiency of Cur delivery to the artificial skin and mechanical strength of the developed films, where a composition containing shea butter and Capmul MCM EP exhibited the highest values of 233.2 ± 96.6 μg/cm2/mg and 4.86 ± 0.14 MPa, respectively.
30142524	5	73	theme	vacuum	929:934	arg1	filtration					936:945	vacuum filtration	929:945	vacuum filtration of the NLCs and CNF mixture, which were confirmed by TEM, SEM, AFM, XPS, and FTIR analyses	929:1036	The Cur-loaded lipid-hybridized CNF (lipid@CNF) films with a smooth rather than particle-embedded surface were obtained by vacuum filtration of the NLCs and CNF mixture, which were confirmed by TEM, SEM, AFM, XPS, and FTIR analyses.
30142524	4	74	dep	skin	602:605	arg1	the					587:589	the	587:589	the	587:589	The lipid composition of the NLCs was optimized based on the efficiency of Cur delivery to the artificial skin and mechanical strength of the developed films, where a composition containing shea butter and Capmul MCM EP exhibited the highest values of 233.2 ± 96.6 μg/cm2/mg and 4.86 ± 0.14 MPa, respectively.
30142524	8	75	theme	skin	1732:1735	arg1	effect					1747:1752	the skin hydration effect	1728:1752	the skin hydration effect of the films	1728:1765	These results could be attributed to the enhanced Cur deposition along with the skin hydration effect of the films.
30142524	6	76	theme	PXRD	1310:1313	arg1	analyses					1315:1322	the DSC and PXRD analyses	1298:1322	analyses	1315:1322	The Cur-loaded lipid@CNF films exhibited more than 2.0-fold increases in Cur deposition to the epidermis of imiquimod (IMQ)-induced psoriatic mouse compared with the films without lipids, which potentially resulted from the amorphous state of Cur observed in the DSC and PXRD analyses and the permeation-enhancing effect of lipids.
30142524	7	77	theme	IMQ-induced	1500:1510	arg1	mice					1512:1515	IMQ-induced mice	1500:1515	IMQ-induced mice	1500:1515	The in vivo anti-psoriatic efficacy test revealed that the Cur-loaded lipid@CNF films ameliorated the psoriatic skin symptoms in IMQ-induced mice, reducing the pro-inflammatory cytokine levels in the skin almost comparable to a commercially available topical corticosteroid cream.
30142524	4	78	theme	NLCs	525:528	arg1	composition					506:516	The lipid composition	496:516	The lipid composition of the NLCs	496:528	The lipid composition of the NLCs was optimized based on the efficiency of Cur delivery to the artificial skin and mechanical strength of the developed films, where a composition containing shea butter and Capmul MCM EP exhibited the highest values of 233.2 ± 96.6 μg/cm2/mg and 4.86 ± 0.14 MPa, respectively.
30142524	6	79	theme	DSC	1302:1304	arg1	analyses					1315:1322	the DSC and PXRD analyses	1298:1322	analyses	1315:1322	The Cur-loaded lipid@CNF films exhibited more than 2.0-fold increases in Cur deposition to the epidermis of imiquimod (IMQ)-induced psoriatic mouse compared with the films without lipids, which potentially resulted from the amorphous state of Cur observed in the DSC and PXRD analyses and the permeation-enhancing effect of lipids.
30142524	5	80	theme	CNF	963:965	arg1	mixture					967:973	CNF mixture	963:973	CNF mixture	963:973	The Cur-loaded lipid-hybridized CNF (lipid@CNF) films with a smooth rather than particle-embedded surface were obtained by vacuum filtration of the NLCs and CNF mixture, which were confirmed by TEM, SEM, AFM, XPS, and FTIR analyses.
30142524	4	81	theme	233.2 ± 96.6 μg/cm2/mg	748:769	arg1	values					738:743	the highest values	726:743	the highest values of 233.2 ± 96.6 μg/cm2/mg and 4.86 ± 0.14 MPa	726:789	The lipid composition of the NLCs was optimized based on the efficiency of Cur delivery to the artificial skin and mechanical strength of the developed films, where a composition containing shea butter and Capmul MCM EP exhibited the highest values of 233.2 ± 96.6 μg/cm2/mg and 4.86 ± 0.14 MPa, respectively.
30142524	4	82	theme	lipid	500:504	arg1	composition					506:516	The lipid composition	496:516	The lipid composition of the NLCs	496:528	The lipid composition of the NLCs was optimized based on the efficiency of Cur delivery to the artificial skin and mechanical strength of the developed films, where a composition containing shea butter and Capmul MCM EP exhibited the highest values of 233.2 ± 96.6 μg/cm2/mg and 4.86 ± 0.14 MPa, respectively.
30142524	4	83	theme	4.86 ± 0.14 MPa	775:789	arg1	values					738:743	the highest values	726:743	the highest values of 233.2 ± 96.6 μg/cm2/mg and 4.86 ± 0.14 MPa	726:789	The lipid composition of the NLCs was optimized based on the efficiency of Cur delivery to the artificial skin and mechanical strength of the developed films, where a composition containing shea butter and Capmul MCM EP exhibited the highest values of 233.2 ± 96.6 μg/cm2/mg and 4.86 ± 0.14 MPa, respectively.
30142524	0	84	theme	lipid-hybridized	16:31	arg1	film					53:56	Curcumin-loaded lipid-hybridized cellulose nanofiber film	0:56	Curcumin-loaded lipid-hybridized cellulose nanofiber film	0:56	Curcumin-loaded lipid-hybridized cellulose nanofiber film ameliorates imiquimod-induced psoriasis-like dermatitis in mice.
30142524	1	85	theme	Cellulose	123:131	arg1	nanofiber					133:141	Cellulose nanofiber	123:141	Cellulose nanofiber (CNF)	123:147	Cellulose nanofiber (CNF) is an attractive biomaterial given its film-forming properties, excellent mechanical properties and biocompatibility.
30142524	1	85	theme	Cellulose	123:131	arg1	biomaterial					166:176	an attractive biomaterial	152:176	an attractive biomaterial given its film-forming properties, excellent mechanical properties and biocompatibility	152:264	Cellulose nanofiber (CNF) is an attractive biomaterial given its film-forming properties, excellent mechanical properties and biocompatibility.
30142524	1	85	theme	Cellulose	123:131	arg1	CNF					144:146	CNF	144:146	CNF	144:146	Cellulose nanofiber (CNF) is an attractive biomaterial given its film-forming properties, excellent mechanical properties and biocompatibility.
30142524	6	86	from	increases	1099:1107	arg1	deposition					1116:1125	Cur deposition	1112:1125	Cur deposition to the epidermis of imiquimod (IMQ)-induced psoriatic mouse	1112:1185	The Cur-loaded lipid@CNF films exhibited more than 2.0-fold increases in Cur deposition to the epidermis of imiquimod (IMQ)-induced psoriatic mouse compared with the films without lipids, which potentially resulted from the amorphous state of Cur observed in the DSC and PXRD analyses and the permeation-enhancing effect of lipids.
30142524	0	87	theme	nanofiber	43:51	arg1	film					53:56	Curcumin-loaded lipid-hybridized cellulose nanofiber film	0:56	Curcumin-loaded lipid-hybridized cellulose nanofiber film	0:56	Curcumin-loaded lipid-hybridized cellulose nanofiber film ameliorates imiquimod-induced psoriasis-like dermatitis in mice.
30142524	5	88	theme	CNF	838:840	arg1	films					854:858	The Cur-loaded lipid-hybridized CNF (lipid@CNF) films	806:858	The Cur-loaded lipid-hybridized CNF (lipid@CNF) films with a smooth rather than particle-embedded surface	806:910	The Cur-loaded lipid-hybridized CNF (lipid@CNF) films with a smooth rather than particle-embedded surface were obtained by vacuum filtration of the NLCs and CNF mixture, which were confirmed by TEM, SEM, AFM, XPS, and FTIR analyses.
30142524	7	89	from	symptoms	1488:1495	arg1	mice					1512:1515	IMQ-induced mice	1500:1515	IMQ-induced mice	1500:1515	The in vivo anti-psoriatic efficacy test revealed that the Cur-loaded lipid@CNF films ameliorated the psoriatic skin symptoms in IMQ-induced mice, reducing the pro-inflammatory cytokine levels in the skin almost comparable to a commercially available topical corticosteroid cream.
30142524	5	90	theme	NLCs	954:957	arg1	filtration					936:945	vacuum filtration	929:945	vacuum filtration of the NLCs and CNF mixture, which were confirmed by TEM, SEM, AFM, XPS, and FTIR analyses	929:1036	The Cur-loaded lipid-hybridized CNF (lipid@CNF) films with a smooth rather than particle-embedded surface were obtained by vacuum filtration of the NLCs and CNF mixture, which were confirmed by TEM, SEM, AFM, XPS, and FTIR analyses.
30142524	7	91	theme	topical	1622:1628	arg1	cream					1645:1649	a commercially available topical corticosteroid cream	1597:1649	a commercially available topical corticosteroid cream	1597:1649	The in vivo anti-psoriatic efficacy test revealed that the Cur-loaded lipid@CNF films ameliorated the psoriatic skin symptoms in IMQ-induced mice, reducing the pro-inflammatory cytokine levels in the skin almost comparable to a commercially available topical corticosteroid cream.
30142524	4	92	theme	Cur	571:573	arg1	delivery					575:582	Cur delivery	571:582	Cur delivery to the artificial skin and mechanical strength of the developed films, where a composition containing shea butter and Capmul MCM EP exhibited the highest values of 233.2 ± 96.6 μg/cm2/mg and 4.86 ± 0.14 MPa, respectively	571:803	The lipid composition of the NLCs was optimized based on the efficiency of Cur delivery to the artificial skin and mechanical strength of the developed films, where a composition containing shea butter and Capmul MCM EP exhibited the highest values of 233.2 ± 96.6 μg/cm2/mg and 4.86 ± 0.14 MPa, respectively.
30142524	5	93	with	films	854:858	arg1	surface					904:910	a smooth rather than particle-embedded surface	865:910	a smooth rather than particle-embedded surface	865:910	The Cur-loaded lipid-hybridized CNF (lipid@CNF) films with a smooth rather than particle-embedded surface were obtained by vacuum filtration of the NLCs and CNF mixture, which were confirmed by TEM, SEM, AFM, XPS, and FTIR analyses.
30142524	3	94	theme	diffusion	478:486	arg1	method					488:493	a solvent diffusion method	468:493	a solvent diffusion method	468:493	NLCs with a mean diameter of ≈500 nm were fabricated by using a solvent diffusion method.
30142524	7	95	theme	skin	1483:1486	arg1	symptoms					1488:1495	the psoriatic skin symptoms	1469:1495	the psoriatic skin symptoms in IMQ-induced mice, reducing the pro-inflammatory cytokine levels in the skin almost comparable to a commercially available topical corticosteroid cream	1469:1649	The in vivo anti-psoriatic efficacy test revealed that the Cur-loaded lipid@CNF films ameliorated the psoriatic skin symptoms in IMQ-induced mice, reducing the pro-inflammatory cytokine levels in the skin almost comparable to a commercially available topical corticosteroid cream.
30142524	0	96	theme	psoriasis-like	88:101	arg1	dermatitis					103:112	imiquimod-induced psoriasis-like dermatitis	70:112	imiquimod-induced psoriasis-like dermatitis in mice	70:120	Curcumin-loaded lipid-hybridized cellulose nanofiber film ameliorates imiquimod-induced psoriasis-like dermatitis in mice.
30142524	3	97	theme	mean	418:421	arg1	diameter					423:430	a mean diameter	416:430	a mean diameter of ≈500 nm	416:441	NLCs with a mean diameter of ≈500 nm were fabricated by using a solvent diffusion method.
30142524	2	98	theme	-loaded	360:366	arg1	carriers					389:396	nanostructured lipid carriers	368:396	curcumin (Cur)-loaded nanostructured lipid carriers (NLCs)	346:403	Herein, CNF film was prepared as a topical drug delivery system by hybridizing curcumin (Cur)-loaded nanostructured lipid carriers (NLCs).
30142524	2	98	theme	-loaded	360:366	arg1	NLCs					399:402	NLCs	399:402	NLCs	399:402	Herein, CNF film was prepared as a topical drug delivery system by hybridizing curcumin (Cur)-loaded nanostructured lipid carriers (NLCs).
30142524	9	99	used	used	1827:1830	arg2	system					1869:1874	a promising topical drug delivery system	1835:1874	a promising topical drug delivery system for psoriasis therapy	1835:1896	These findings suggest that the developed CNF films can be used as a promising topical drug delivery system for psoriasis therapy.
30142524	9	99	used	used	1827:1830	arg2	films					1814:1818	the developed CNF films	1796:1818	the developed CNF films	1796:1818	These findings suggest that the developed CNF films can be used as a promising topical drug delivery system for psoriasis therapy.
30142524	9	100	theme	CNF	1810:1812	arg1	films					1814:1818	the developed CNF films	1796:1818	the developed CNF films	1796:1818	These findings suggest that the developed CNF films can be used as a promising topical drug delivery system for psoriasis therapy.
30142524	9	100	theme	CNF	1810:1812	arg1	system					1869:1874	a promising topical drug delivery system	1835:1874	a promising topical drug delivery system for psoriasis therapy	1835:1896	These findings suggest that the developed CNF films can be used as a promising topical drug delivery system for psoriasis therapy.
30142524	7	101	theme	@	1446:1446	arg1	films					1451:1455	the Cur-loaded lipid@CNF films	1426:1455	the Cur-loaded lipid@CNF films	1426:1455	The in vivo anti-psoriatic efficacy test revealed that the Cur-loaded lipid@CNF films ameliorated the psoriatic skin symptoms in IMQ-induced mice, reducing the pro-inflammatory cytokine levels in the skin almost comparable to a commercially available topical corticosteroid cream.
30142524	6	102	theme	CNF	1060:1062	arg1	films					1064:1068	The Cur-loaded lipid@CNF films	1039:1068	The Cur-loaded lipid@CNF films	1039:1068	The Cur-loaded lipid@CNF films exhibited more than 2.0-fold increases in Cur deposition to the epidermis of imiquimod (IMQ)-induced psoriatic mouse compared with the films without lipids, which potentially resulted from the amorphous state of Cur observed in the DSC and PXRD analyses and the permeation-enhancing effect of lipids.
30142524	5	103	theme	lipid-hybridized	821:836	arg1	CNF					838:840	Cur-loaded lipid-hybridized CNF	810:840	The Cur-loaded lipid-hybridized CNF (lipid@CNF) films with a smooth rather than particle-embedded surface	806:910	The Cur-loaded lipid-hybridized CNF (lipid@CNF) films with a smooth rather than particle-embedded surface were obtained by vacuum filtration of the NLCs and CNF mixture, which were confirmed by TEM, SEM, AFM, XPS, and FTIR analyses.
30142524	5	103	theme	lipid-hybridized	821:836	arg1	CNF					849:851	lipid@CNF	843:851	lipid@CNF	843:851	The Cur-loaded lipid-hybridized CNF (lipid@CNF) films with a smooth rather than particle-embedded surface were obtained by vacuum filtration of the NLCs and CNF mixture, which were confirmed by TEM, SEM, AFM, XPS, and FTIR analyses.
30547854	14	0	theme	strains	1383:1389	arg1	classification					1357:1370	the classification	1353:1370	the classification of the two strains into the genus Microbacterium	1353:1419	These results are consistent with the classification of the two strains into the genus Microbacterium.
30547854	15	1	theme	species	1538:1544	arg1	representatives					1511:1525	representatives	1511:1525	representatives of a novel species in the genus Microbacterium, for which the name Microbacterium suaedae sp	1511:1618	On the basis of the evidence presented in this study, strains YZYP 306T and YZGP 509 are representatives of a novel species in the genus Microbacterium, for which the name Microbacterium suaedae sp.
30547854	15	1	theme	species	1538:1544	arg1	strains					1476:1482	strains	1476:1482	strains YZYP 306T and YZGP 509	1476:1505	On the basis of the evidence presented in this study, strains YZYP 306T and YZGP 509 are representatives of a novel species in the genus Microbacterium, for which the name Microbacterium suaedae sp.
30547854	15	2	from	species	1538:1544	arg1	Microbacterium					1559:1572	the genus Microbacterium	1549:1572	the genus Microbacterium	1549:1572	On the basis of the evidence presented in this study, strains YZYP 306T and YZGP 509 are representatives of a novel species in the genus Microbacterium, for which the name Microbacterium suaedae sp.
30547854	3	3	dep	Gram-stain-positive	259:277	arg1	aerobic					280:286	aerobic	280:286	aerobic	280:286	Cells were Gram-stain-positive, aerobic, non-motile, short rods.
30547854	3	3	dep	Gram-stain-positive	259:277	arg1	short					301:305	short	301:305	short	301:305	Cells were Gram-stain-positive, aerobic, non-motile, short rods.
30547854	3	3	dep	Gram-stain-positive	259:277	arg1	non-motile					289:298	non-motile	289:298	non-motile	289:298	Cells were Gram-stain-positive, aerobic, non-motile, short rods.
30547854	5	4	dep	Microbacterium	656:669	arg1	sorbitolivorans					671:685	sorbitolivorans	671:685	sorbitolivorans	671:685	Phylogenetic analyses of the 16S rRNA gene sequences placed the two strains within the genus Microbacterium with the highest similarities to Microbacterium indicum BBH6T (97.8 %) and Microbacterium sorbitolivorans SZDIS-1-1T (97.2 %).
30547854	4	5	theme	strain	353:358	arg1	YZGP					360:363	strain YZGP 509	353:367	strain YZGP 509	353:367	Strain YZYP 306T grew at 4-40 °C, while strain YZGP 509 grew at 4-42 °C, with optimum growth at 28 °C, and they both grew at pH 6.0-12.0 and 0-15 % (w/v) NaCl.
30547854	17	6	theme	1.16261T=KCTC	1676:1688	arg1	306T					1663:1666	YZYP 306T	1658:1666	YZYP 306T (=CGMCC 1.16261T=KCTC 49101T)	1658:1696	The type strain is YZYP 306T (=CGMCC 1.16261T=KCTC 49101T).
30547854	17	6	theme	1.16261T=KCTC	1676:1688	arg1	49101T					1690:1695	=CGMCC 1.16261T=KCTC 49101T	1669:1695	=CGMCC 1.16261T=KCTC 49101T	1669:1695	The type strain is YZYP 306T (=CGMCC 1.16261T=KCTC 49101T).
30547854	5	7	theme	Microbacterium	614:627	arg1	BBH6T					637:641	Microbacterium indicum BBH6T	614:641	Microbacterium indicum BBH6T (97.8 %)	614:650	Phylogenetic analyses of the 16S rRNA gene sequences placed the two strains within the genus Microbacterium with the highest similarities to Microbacterium indicum BBH6T (97.8 %) and Microbacterium sorbitolivorans SZDIS-1-1T (97.2 %).
30547854	5	7	theme	Microbacterium	614:627	arg1	%					649:649	97.8 %	644:649	97.8 %	644:649	Phylogenetic analyses of the 16S rRNA gene sequences placed the two strains within the genus Microbacterium with the highest similarities to Microbacterium indicum BBH6T (97.8 %) and Microbacterium sorbitolivorans SZDIS-1-1T (97.2 %).
30547854	7	8	theme	genomic	801:807	arg1	68.49					865:869	68.49	865:869	68.49	865:869	The genomic DNA G+C contents of strains YZYP 306T and YZGP 509 were 68.49 and 68.53 mol%, respectively.
30547854	7	8	theme	genomic	801:807	arg1	contents					817:824	The genomic DNA G+C contents	797:824	The genomic DNA G+C contents of strains YZYP 306T and YZGP 509	797:858	The genomic DNA G+C contents of strains YZYP 306T and YZGP 509 were 68.49 and 68.53 mol%, respectively.
30547854	7	9	theme	YZYP	837:840	arg1	306T					842:845	YZYP 306T	837:845	YZYP 306T	837:845	The genomic DNA G+C contents of strains YZYP 306T and YZGP 509 were 68.49 and 68.53 mol%, respectively.
30547854	7	9	theme	YZYP	837:840	arg1	strains					829:835	strains YZYP 306T and YZGP 509	829:858	strains YZYP 306T and YZGP 509	829:858	The genomic DNA G+C contents of strains YZYP 306T and YZGP 509 were 68.49 and 68.53 mol%, respectively.
30547854	1	10	theme	Suaeda	47:52	arg1	aralocaspica					54:65	Suaeda aralocaspica	47:65	Suaeda aralocaspica	47:65	nov., isolated from Suaeda aralocaspica.
30547854	15	11	theme	genus	1553:1557	arg1	Microbacterium					1559:1572	the genus Microbacterium	1549:1572	the genus Microbacterium	1549:1572	On the basis of the evidence presented in this study, strains YZYP 306T and YZGP 509 are representatives of a novel species in the genus Microbacterium, for which the name Microbacterium suaedae sp.
30547854	5	12	theme	indicum	629:635	arg1	BBH6T					637:641	Microbacterium indicum BBH6T	614:641	Microbacterium indicum BBH6T (97.8 %)	614:650	Phylogenetic analyses of the 16S rRNA gene sequences placed the two strains within the genus Microbacterium with the highest similarities to Microbacterium indicum BBH6T (97.8 %) and Microbacterium sorbitolivorans SZDIS-1-1T (97.2 %).
30547854	5	12	theme	indicum	629:635	arg1	%					649:649	97.8 %	644:649	97.8 %	644:649	Phylogenetic analyses of the 16S rRNA gene sequences placed the two strains within the genus Microbacterium with the highest similarities to Microbacterium indicum BBH6T (97.8 %) and Microbacterium sorbitolivorans SZDIS-1-1T (97.2 %).
30547854	5	13	theme	highest	590:596	arg1	similarities					598:609	the highest similarities	586:609	the highest similarities to Microbacterium indicum BBH6T (97.8 %) and Microbacterium sorbitolivorans SZDIS-1-1T (97.2 %)	586:705	Phylogenetic analyses of the 16S rRNA gene sequences placed the two strains within the genus Microbacterium with the highest similarities to Microbacterium indicum BBH6T (97.8 %) and Microbacterium sorbitolivorans SZDIS-1-1T (97.2 %).
30547854	7	14	theme	68.53 mol	875:883	arg1	%					884:884	68.53 mol%	875:884	68.53 mol%	875:884	The genomic DNA G+C contents of strains YZYP 306T and YZGP 509 were 68.49 and 68.53 mol%, respectively.
30547854	14	15	theme	genus	1400:1404	arg1	Microbacterium					1406:1419	the genus Microbacterium	1396:1419	the genus Microbacterium	1396:1419	These results are consistent with the classification of the two strains into the genus Microbacterium.
30547854	14	16	with	consistent	1337:1346	arg1	classification					1357:1370	the classification	1353:1370	the classification of the two strains into the genus Microbacterium	1353:1419	These results are consistent with the classification of the two strains into the genus Microbacterium.
30547854	5	17	theme	rRNA	506:509	arg1	sequences					516:524	the 16S rRNA gene sequences	498:524	the 16S rRNA gene sequences	498:524	Phylogenetic analyses of the 16S rRNA gene sequences placed the two strains within the genus Microbacterium with the highest similarities to Microbacterium indicum BBH6T (97.8 %) and Microbacterium sorbitolivorans SZDIS-1-1T (97.2 %).
30547854	5	18	theme	16S	502:504	arg1	sequences					516:524	the 16S rRNA gene sequences	498:524	the 16S rRNA gene sequences	498:524	Phylogenetic analyses of the 16S rRNA gene sequences placed the two strains within the genus Microbacterium with the highest similarities to Microbacterium indicum BBH6T (97.8 %) and Microbacterium sorbitolivorans SZDIS-1-1T (97.2 %).
30547854	15	19	from	Microbacterium	1559:1572	arg1	representatives					1511:1525	representatives	1511:1525	representatives of a novel species in the genus Microbacterium, for which the name Microbacterium suaedae sp	1511:1618	On the basis of the evidence presented in this study, strains YZYP 306T and YZGP 509 are representatives of a novel species in the genus Microbacterium, for which the name Microbacterium suaedae sp.
30547854	15	19	from	Microbacterium	1559:1572	arg1	strains					1476:1482	strains	1476:1482	strains YZYP 306T and YZGP 509	1476:1505	On the basis of the evidence presented in this study, strains YZYP 306T and YZGP 509 are representatives of a novel species in the genus Microbacterium, for which the name Microbacterium suaedae sp.
30547854	5	20	with	Microbacterium	566:579	arg1	similarities					598:609	the highest similarities	586:609	the highest similarities to Microbacterium indicum BBH6T (97.8 %) and Microbacterium sorbitolivorans SZDIS-1-1T (97.2 %)	586:705	Phylogenetic analyses of the 16S rRNA gene sequences placed the two strains within the genus Microbacterium with the highest similarities to Microbacterium indicum BBH6T (97.8 %) and Microbacterium sorbitolivorans SZDIS-1-1T (97.2 %).
30547854	15	21	theme	novel	1532:1536	arg1	species					1538:1544	a novel species	1530:1544	a novel species	1530:1544	On the basis of the evidence presented in this study, strains YZYP 306T and YZGP 509 are representatives of a novel species in the genus Microbacterium, for which the name Microbacterium suaedae sp.
30547854	4	22	from	°C	412:413	arg1	growth					399:404	optimum growth	391:404	optimum growth at 28 °C	391:413	Strain YZYP 306T grew at 4-40 °C, while strain YZGP 509 grew at 4-42 °C, with optimum growth at 28 °C, and they both grew at pH 6.0-12.0 and 0-15 % (w/v) NaCl.
30547854	7	23	theme	DNA	809:811	arg1	68.49					865:869	68.49	865:869	68.49	865:869	The genomic DNA G+C contents of strains YZYP 306T and YZGP 509 were 68.49 and 68.53 mol%, respectively.
30547854	7	23	theme	DNA	809:811	arg1	contents					817:824	The genomic DNA G+C contents	797:824	The genomic DNA G+C contents of strains YZYP 306T and YZGP 509	797:858	The genomic DNA G+C contents of strains YZYP 306T and YZGP 509 were 68.49 and 68.53 mol%, respectively.
30547854	0	24	theme	suaedae	15:21	arg1	sp					23:24	Microbacterium suaedae sp	0:24	Microbacterium suaedae sp.	0:25	Microbacterium suaedae sp.
30547854	5	25	theme	sequences	516:524	arg1	analyses					486:493	Phylogenetic analyses	473:493	Phylogenetic analyses of the 16S rRNA gene sequences	473:524	Phylogenetic analyses of the 16S rRNA gene sequences placed the two strains within the genus Microbacterium with the highest similarities to Microbacterium indicum BBH6T (97.8 %) and Microbacterium sorbitolivorans SZDIS-1-1T (97.2 %).
30547854	7	26	theme	G+C	813:815	arg1	68.49					865:869	68.49	865:869	68.49	865:869	The genomic DNA G+C contents of strains YZYP 306T and YZGP 509 were 68.49 and 68.53 mol%, respectively.
30547854	7	26	theme	G+C	813:815	arg1	contents					817:824	The genomic DNA G+C contents	797:824	The genomic DNA G+C contents of strains YZYP 306T and YZGP 509	797:858	The genomic DNA G+C contents of strains YZYP 306T and YZGP 509 were 68.49 and 68.53 mol%, respectively.
30547854	0	27	theme	Microbacterium	0:13	arg1	sp					23:24	Microbacterium suaedae sp	0:24	Microbacterium suaedae sp.	0:25	Microbacterium suaedae sp.
30547854	4	28	theme	%	459:459	arg1	NaCl					467:470	0-15 % (w/v) NaCl	454:470	0-15 % (w/v) NaCl	454:470	Strain YZYP 306T grew at 4-40 °C, while strain YZGP 509 grew at 4-42 °C, with optimum growth at 28 °C, and they both grew at pH 6.0-12.0 and 0-15 % (w/v) NaCl.
30547854	8	29	theme	cell-wall	920:928	arg1	ornithine					945:953	ornithine	945:953	ornithine	945:953	The characteristic cell-wall amino acid was ornithine.
30547854	8	29	theme	cell-wall	920:928	arg1	acid					936:939	The characteristic cell-wall amino acid	901:939	The characteristic cell-wall amino acid	901:939	The characteristic cell-wall amino acid was ornithine.
30547854	15	30	dep	evidence	1442:1449	arg1	basis					1429:1433	basis	1429:1433	basis	1429:1433	On the basis of the evidence presented in this study, strains YZYP 306T and YZGP 509 are representatives of a novel species in the genus Microbacterium, for which the name Microbacterium suaedae sp.
30547854	15	30	dep	evidence	1442:1449	arg1	the					1425:1427	the	1425:1427	the	1425:1427	On the basis of the evidence presented in this study, strains YZYP 306T and YZGP 509 are representatives of a novel species in the genus Microbacterium, for which the name Microbacterium suaedae sp.
30547854	6	31	theme	identity	731:738	arg1	%					794:794	78.3 %	789:794	78.3 %	789:794	The average nucleotide identity value between YZYP 306T and M. indicum BBH6T was 78.3 %.
30547854	6	31	theme	identity	731:738	arg1	value					740:744	The average nucleotide identity value	708:744	The average nucleotide identity value between YZYP 306T and M. indicum BBH6T	708:783	The average nucleotide identity value between YZYP 306T and M. indicum BBH6T was 78.3 %.
30547854	9	32	theme	Whole-cell	956:965	arg1	sugars					967:972	Whole-cell sugars	956:972	Whole-cell sugars	956:972	Whole-cell sugars were galactose, mannose and ribose.
30547854	4	33	theme	0-15 	454:458	arg1	NaCl					467:470	0-15 % (w/v) NaCl	454:470	0-15 % (w/v) NaCl	454:470	Strain YZYP 306T grew at 4-40 °C, while strain YZGP 509 grew at 4-42 °C, with optimum growth at 28 °C, and they both grew at pH 6.0-12.0 and 0-15 % (w/v) NaCl.
30547854	17	34	theme	type	1643:1646	arg1	strain					1648:1653	The type strain	1639:1653	The type strain	1639:1653	The type strain is YZYP 306T (=CGMCC 1.16261T=KCTC 49101T).
30547854	17	34	theme	type	1643:1646	arg1	306T					1663:1666	YZYP 306T	1658:1666	YZYP 306T (=CGMCC 1.16261T=KCTC 49101T)	1658:1696	The type strain is YZYP 306T (=CGMCC 1.16261T=KCTC 49101T).
30547854	5	35	theme	Phylogenetic	473:484	arg1	analyses					486:493	Phylogenetic analyses	473:493	Phylogenetic analyses of the 16S rRNA gene sequences	473:524	Phylogenetic analyses of the 16S rRNA gene sequences placed the two strains within the genus Microbacterium with the highest similarities to Microbacterium indicum BBH6T (97.8 %) and Microbacterium sorbitolivorans SZDIS-1-1T (97.2 %).
30547854	8	36	theme	characteristic	905:918	arg1	ornithine					945:953	ornithine	945:953	ornithine	945:953	The characteristic cell-wall amino acid was ornithine.
30547854	8	36	theme	characteristic	905:918	arg1	acid					936:939	The characteristic cell-wall amino acid	901:939	The characteristic cell-wall amino acid	901:939	The characteristic cell-wall amino acid was ornithine.
30547854	7	37	theme	strains	829:835	arg1	68.49					865:869	68.49	865:869	68.49	865:869	The genomic DNA G+C contents of strains YZYP 306T and YZGP 509 were 68.49 and 68.53 mol%, respectively.
30547854	7	37	theme	strains	829:835	arg1	contents					817:824	The genomic DNA G+C contents	797:824	The genomic DNA G+C contents of strains YZYP 306T and YZGP 509	797:858	The genomic DNA G+C contents of strains YZYP 306T and YZGP 509 were 68.49 and 68.53 mol%, respectively.
30547854	11	38	theme	major	1063:1067	arg1	acids					1084:1088	The major cellular fatty acids	1059:1088	The major cellular fatty acids	1059:1088	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
30547854	11	38	theme	major	1063:1067	arg1	anteiso-C15 					1095:1106	anteiso-C15 	1095:1106	anteiso-C15 	1095:1106	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
30547854	4	39	theme	w/v	462:464	arg1	NaCl					467:470	0-15 % (w/v) NaCl	454:470	0-15 % (w/v) NaCl	454:470	Strain YZYP 306T grew at 4-40 °C, while strain YZGP 509 grew at 4-42 °C, with optimum growth at 28 °C, and they both grew at pH 6.0-12.0 and 0-15 % (w/v) NaCl.
30547854	3	40	theme	Gram-stain-positive	259:277	arg1	rods					307:310	Gram-stain-positive, aerobic, non-motile, short rods	259:310	Gram-stain-positive, aerobic, non-motile, short rods	259:310	Cells were Gram-stain-positive, aerobic, non-motile, short rods.
30547854	15	41	dep	strains	1476:1482	arg1	306T					1489:1492	YZYP 306T	1484:1492	YZYP 306T	1484:1492	On the basis of the evidence presented in this study, strains YZYP 306T and YZGP 509 are representatives of a novel species in the genus Microbacterium, for which the name Microbacterium suaedae sp.
30547854	15	41	dep	strains	1476:1482	arg1	strains					1476:1482	strains	1476:1482	strains YZYP 306T and YZGP 509	1476:1505	On the basis of the evidence presented in this study, strains YZYP 306T and YZGP 509 are representatives of a novel species in the genus Microbacterium, for which the name Microbacterium suaedae sp.
30547854	15	41	dep	strains	1476:1482	arg1	YZGP					1498:1501	YZGP 509	1498:1505	YZGP 509	1498:1505	On the basis of the evidence presented in this study, strains YZYP 306T and YZGP 509 are representatives of a novel species in the genus Microbacterium, for which the name Microbacterium suaedae sp.
30547854	15	42	theme	Microbacterium	1594:1607	arg1	sp					1617:1618	the name Microbacterium suaedae sp	1585:1618	the name Microbacterium suaedae sp	1585:1618	On the basis of the evidence presented in this study, strains YZYP 306T and YZGP 509 are representatives of a novel species in the genus Microbacterium, for which the name Microbacterium suaedae sp.
30547854	5	43	theme	Microbacterium	656:669	arg1	SZDIS-1-1T					687:696	Microbacterium sorbitolivorans SZDIS-1-1T	656:696	Microbacterium sorbitolivorans SZDIS-1-1T (97.2 %)	656:705	Phylogenetic analyses of the 16S rRNA gene sequences placed the two strains within the genus Microbacterium with the highest similarities to Microbacterium indicum BBH6T (97.8 %) and Microbacterium sorbitolivorans SZDIS-1-1T (97.2 %).
30547854	5	43	theme	Microbacterium	656:669	arg1	%					704:704	97.2 %	699:704	97.2 %	699:704	Phylogenetic analyses of the 16S rRNA gene sequences placed the two strains within the genus Microbacterium with the highest similarities to Microbacterium indicum BBH6T (97.8 %) and Microbacterium sorbitolivorans SZDIS-1-1T (97.2 %).
30547854	11	44	theme	cellular	1069:1076	arg1	acids					1084:1088	The major cellular fatty acids	1059:1088	The major cellular fatty acids	1059:1088	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
30547854	11	44	theme	cellular	1069:1076	arg1	anteiso-C15 					1095:1106	anteiso-C15 	1095:1106	anteiso-C15 	1095:1106	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
30547854	2	45	theme	Suaeda	148:153	arg1	aralocaspica					155:166	the halophyte Suaeda aralocaspica	134:166	the halophyte Suaeda aralocaspica collected from the southern edge of the Gurbantunggut desert	134:227	Two bacterial strains, YZYP 306T and YZGP 509, were isolated from the halophyte Suaeda aralocaspica collected from the southern edge of the Gurbantunggut desert, north-west China.
30547854	2	45	theme	Suaeda	148:153	arg1	China					241:245	north-west China	230:245	north-west China	230:245	Two bacterial strains, YZYP 306T and YZGP 509, were isolated from the halophyte Suaeda aralocaspica collected from the southern edge of the Gurbantunggut desert, north-west China.
30547854	2	46	theme	Gurbantunggut	208:220	arg1	desert					222:227	the Gurbantunggut desert	204:227	the Gurbantunggut desert	204:227	Two bacterial strains, YZYP 306T and YZGP 509, were isolated from the halophyte Suaeda aralocaspica collected from the southern edge of the Gurbantunggut desert, north-west China.
30547854	15	47	theme	suaedae	1609:1615	arg1	sp					1617:1618	the name Microbacterium suaedae sp	1585:1618	the name Microbacterium suaedae sp	1585:1618	On the basis of the evidence presented in this study, strains YZYP 306T and YZGP 509 are representatives of a novel species in the genus Microbacterium, for which the name Microbacterium suaedae sp.
30547854	4	48	dep	grew	430:433	arg1	both					425:428	both	425:428	both	425:428	Strain YZYP 306T grew at 4-40 °C, while strain YZGP 509 grew at 4-42 °C, with optimum growth at 28 °C, and they both grew at pH 6.0-12.0 and 0-15 % (w/v) NaCl.
30547854	11	49	theme	fatty	1078:1082	arg1	acids					1084:1088	The major cellular fatty acids	1059:1088	The major cellular fatty acids	1059:1088	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
30547854	11	49	theme	fatty	1078:1082	arg1	anteiso-C15 					1095:1106	anteiso-C15 	1095:1106	anteiso-C15 	1095:1106	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
30547854	2	50	theme	halophyte	138:146	arg1	aralocaspica					155:166	the halophyte Suaeda aralocaspica	134:166	the halophyte Suaeda aralocaspica collected from the southern edge of the Gurbantunggut desert	134:227	Two bacterial strains, YZYP 306T and YZGP 509, were isolated from the halophyte Suaeda aralocaspica collected from the southern edge of the Gurbantunggut desert, north-west China.
30547854	2	50	theme	halophyte	138:146	arg1	China					241:245	north-west China	230:245	north-west China	230:245	Two bacterial strains, YZYP 306T and YZGP 509, were isolated from the halophyte Suaeda aralocaspica collected from the southern edge of the Gurbantunggut desert, north-west China.
30547854	4	51	theme	YZYP	320:323	arg1	306T					325:328	Strain YZYP 306T	313:328	Strain YZYP 306T	313:328	Strain YZYP 306T grew at 4-40 °C, while strain YZGP 509 grew at 4-42 °C, with optimum growth at 28 °C, and they both grew at pH 6.0-12.0 and 0-15 % (w/v) NaCl.
30547854	10	52	theme	acyl	1014:1017	arg1	glycolyl					1049:1056	glycolyl	1049:1056	glycolyl	1049:1056	The acyl type of the peptidoglycan was glycolyl.
30547854	10	52	theme	acyl	1014:1017	arg1	type					1019:1022	The acyl type	1010:1022	The acyl type of the peptidoglycan	1010:1043	The acyl type of the peptidoglycan was glycolyl.
30547854	17	53	theme	YZYP	1658:1661	arg1	strain					1648:1653	The type strain	1639:1653	The type strain	1639:1653	The type strain is YZYP 306T (=CGMCC 1.16261T=KCTC 49101T).
30547854	17	53	theme	YZYP	1658:1661	arg1	306T					1663:1666	YZYP 306T	1658:1666	YZYP 306T (=CGMCC 1.16261T=KCTC 49101T)	1658:1696	The type strain is YZYP 306T (=CGMCC 1.16261T=KCTC 49101T).
30547854	17	53	theme	YZYP	1658:1661	arg1	49101T					1690:1695	=CGMCC 1.16261T=KCTC 49101T	1669:1695	=CGMCC 1.16261T=KCTC 49101T	1669:1695	The type strain is YZYP 306T (=CGMCC 1.16261T=KCTC 49101T).
30547854	4	54	theme	optimum	391:397	arg1	growth					399:404	optimum growth	391:404	optimum growth at 28 °C	391:413	Strain YZYP 306T grew at 4-40 °C, while strain YZGP 509 grew at 4-42 °C, with optimum growth at 28 °C, and they both grew at pH 6.0-12.0 and 0-15 % (w/v) NaCl.
30547854	4	55	theme	Strain	313:318	arg1	306T					325:328	Strain YZYP 306T	313:328	Strain YZYP 306T	313:328	Strain YZYP 306T grew at 4-40 °C, while strain YZGP 509 grew at 4-42 °C, with optimum growth at 28 °C, and they both grew at pH 6.0-12.0 and 0-15 % (w/v) NaCl.
30547854	2	56	attach	isolated	120:127	arg2	306T					96:99	306T	96:99	306T	96:99	Two bacterial strains, YZYP 306T and YZGP 509, were isolated from the halophyte Suaeda aralocaspica collected from the southern edge of the Gurbantunggut desert, north-west China.
30547854	2	56	attach	isolated	120:127	arg2	strains					82:88	Two bacterial strains	68:88	Two bacterial strains	68:88	Two bacterial strains, YZYP 306T and YZGP 509, were isolated from the halophyte Suaeda aralocaspica collected from the southern edge of the Gurbantunggut desert, north-west China.
30547854	2	56	attach	isolated	120:127	arg1	aralocaspica					155:166	the halophyte Suaeda aralocaspica	134:166	the halophyte Suaeda aralocaspica collected from the southern edge of the Gurbantunggut desert	134:227	Two bacterial strains, YZYP 306T and YZGP 509, were isolated from the halophyte Suaeda aralocaspica collected from the southern edge of the Gurbantunggut desert, north-west China.
30547854	2	56	attach	isolated	120:127	arg2	YZGP					105:108	YZGP	105:108	YZGP	105:108	Two bacterial strains, YZYP 306T and YZGP 509, were isolated from the halophyte Suaeda aralocaspica collected from the southern edge of the Gurbantunggut desert, north-west China.
30547854	2	56	attach	isolated	120:127	arg1	China					241:245	north-west China	230:245	north-west China	230:245	Two bacterial strains, YZYP 306T and YZGP 509, were isolated from the halophyte Suaeda aralocaspica collected from the southern edge of the Gurbantunggut desert, north-west China.
30547854	15	57	theme	evidence	1442:1449	arg1	representatives					1511:1525	representatives	1511:1525	representatives of a novel species in the genus Microbacterium, for which the name Microbacterium suaedae sp	1511:1618	On the basis of the evidence presented in this study, strains YZYP 306T and YZGP 509 are representatives of a novel species in the genus Microbacterium, for which the name Microbacterium suaedae sp.
30547854	15	57	theme	evidence	1442:1449	arg1	strains					1476:1482	strains	1476:1482	strains YZYP 306T and YZGP 509	1476:1505	On the basis of the evidence presented in this study, strains YZYP 306T and YZGP 509 are representatives of a novel species in the genus Microbacterium, for which the name Microbacterium suaedae sp.
30547854	5	58	theme	genus	560:564	arg1	Microbacterium					566:579	the genus Microbacterium	556:579	the genus Microbacterium with the highest similarities to Microbacterium indicum BBH6T (97.8 %) and Microbacterium sorbitolivorans SZDIS-1-1T (97.2 %)	556:705	Phylogenetic analyses of the 16S rRNA gene sequences placed the two strains within the genus Microbacterium with the highest similarities to Microbacterium indicum BBH6T (97.8 %) and Microbacterium sorbitolivorans SZDIS-1-1T (97.2 %).
30547854	2	59	theme	southern	187:194	arg1	edge					196:199	the southern edge	183:199	the southern edge of the Gurbantunggut desert	183:227	Two bacterial strains, YZYP 306T and YZGP 509, were isolated from the halophyte Suaeda aralocaspica collected from the southern edge of the Gurbantunggut desert, north-west China.
30547854	5	60	theme	gene	511:514	arg1	sequences					516:524	the 16S rRNA gene sequences	498:524	the 16S rRNA gene sequences	498:524	Phylogenetic analyses of the 16S rRNA gene sequences placed the two strains within the genus Microbacterium with the highest similarities to Microbacterium indicum BBH6T (97.8 %) and Microbacterium sorbitolivorans SZDIS-1-1T (97.2 %).
30547854	15	61	theme	YZYP	1484:1487	arg1	306T					1489:1492	YZYP 306T	1484:1492	YZYP 306T	1484:1492	On the basis of the evidence presented in this study, strains YZYP 306T and YZGP 509 are representatives of a novel species in the genus Microbacterium, for which the name Microbacterium suaedae sp.
30547854	15	61	theme	YZYP	1484:1487	arg1	strains					1476:1482	strains	1476:1482	strains YZYP 306T and YZGP 509	1476:1505	On the basis of the evidence presented in this study, strains YZYP 306T and YZGP 509 are representatives of a novel species in the genus Microbacterium, for which the name Microbacterium suaedae sp.
30547854	13	62	theme	unidentified	1294:1305	arg1	glycolipid					1307:1316	an unidentified glycolipid	1291:1316	an unidentified glycolipid	1291:1316	The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid and an unidentified glycolipid.
30547854	8	63	theme	amino	930:934	arg1	ornithine					945:953	ornithine	945:953	ornithine	945:953	The characteristic cell-wall amino acid was ornithine.
30547854	8	63	theme	amino	930:934	arg1	acid					936:939	The characteristic cell-wall amino acid	901:939	The characteristic cell-wall amino acid	901:939	The characteristic cell-wall amino acid was ornithine.
30547854	2	64	theme	bacterial	72:80	arg1	YZGP					105:108	YZGP	105:108	YZGP	105:108	Two bacterial strains, YZYP 306T and YZGP 509, were isolated from the halophyte Suaeda aralocaspica collected from the southern edge of the Gurbantunggut desert, north-west China.
30547854	2	64	theme	bacterial	72:80	arg1	strains					82:88	Two bacterial strains	68:88	Two bacterial strains	68:88	Two bacterial strains, YZYP 306T and YZGP 509, were isolated from the halophyte Suaeda aralocaspica collected from the southern edge of the Gurbantunggut desert, north-west China.
30547854	2	64	theme	bacterial	72:80	arg1	306T					96:99	306T	96:99	306T	96:99	Two bacterial strains, YZYP 306T and YZGP 509, were isolated from the halophyte Suaeda aralocaspica collected from the southern edge of the Gurbantunggut desert, north-west China.
30547854	11	65	dep	anteiso-C15 	1095:1106	arg1	 0					1125:1126	 0	1125:1126	 0	1125:1126	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
30547854	11	65	dep	anteiso-C15 	1095:1106	arg1	 0					1108:1109	 0	1108:1109	 0	1108:1109	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
30547854	11	65	dep	anteiso-C15 	1095:1106	arg1	 0					1141:1142	 0	1141:1142	 0	1141:1142	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
30547854	11	65	dep	anteiso-C15 	1095:1106	arg1	iso-C16 					1132:1139	iso-C16 	1132:1139	iso-C16 	1132:1139	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
30547854	6	66	theme	nucleotide	720:729	arg1	%					794:794	78.3 %	789:794	78.3 %	789:794	The average nucleotide identity value between YZYP 306T and M. indicum BBH6T was 78.3 %.
30547854	6	66	theme	nucleotide	720:729	arg1	value					740:744	The average nucleotide identity value	708:744	The average nucleotide identity value between YZYP 306T and M. indicum BBH6T	708:783	The average nucleotide identity value between YZYP 306T and M. indicum BBH6T was 78.3 %.
30547854	12	67	theme	major	1149:1153	arg1	menaquinones					1155:1166	The major menaquinones	1145:1166	The major menaquinones	1145:1166	The major menaquinones were MK-10 and MK-11.
30547854	12	67	theme	major	1149:1153	arg1	MK-10					1173:1177	MK-10	1173:1177	MK-10	1173:1177	The major menaquinones were MK-10 and MK-11.
30547854	15	68	from	representatives	1511:1525	arg1	Microbacterium					1559:1572	the genus Microbacterium	1549:1572	the genus Microbacterium	1549:1572	On the basis of the evidence presented in this study, strains YZYP 306T and YZGP 509 are representatives of a novel species in the genus Microbacterium, for which the name Microbacterium suaedae sp.
30547854	15	69	theme	name	1589:1592	arg1	sp					1617:1618	the name Microbacterium suaedae sp	1585:1618	the name Microbacterium suaedae sp	1585:1618	On the basis of the evidence presented in this study, strains YZYP 306T and YZGP 509 are representatives of a novel species in the genus Microbacterium, for which the name Microbacterium suaedae sp.
30547854	17	70	theme	=CGMCC	1669:1674	arg1	306T					1663:1666	YZYP 306T	1658:1666	YZYP 306T (=CGMCC 1.16261T=KCTC 49101T)	1658:1696	The type strain is YZYP 306T (=CGMCC 1.16261T=KCTC 49101T).
30547854	17	70	theme	=CGMCC	1669:1674	arg1	49101T					1690:1695	=CGMCC 1.16261T=KCTC 49101T	1669:1695	=CGMCC 1.16261T=KCTC 49101T	1669:1695	The type strain is YZYP 306T (=CGMCC 1.16261T=KCTC 49101T).
30547854	6	71	theme	average	712:718	arg1	%					794:794	78.3 %	789:794	78.3 %	789:794	The average nucleotide identity value between YZYP 306T and M. indicum BBH6T was 78.3 %.
30547854	6	71	theme	average	712:718	arg1	value					740:744	The average nucleotide identity value	708:744	The average nucleotide identity value between YZYP 306T and M. indicum BBH6T	708:783	The average nucleotide identity value between YZYP 306T and M. indicum BBH6T was 78.3 %.
30547854	2	72	theme	north-west	230:239	arg1	aralocaspica					155:166	the halophyte Suaeda aralocaspica	134:166	the halophyte Suaeda aralocaspica collected from the southern edge of the Gurbantunggut desert	134:227	Two bacterial strains, YZYP 306T and YZGP 509, were isolated from the halophyte Suaeda aralocaspica collected from the southern edge of the Gurbantunggut desert, north-west China.
30547854	2	72	theme	north-west	230:239	arg1	China					241:245	north-west China	230:245	north-west China	230:245	Two bacterial strains, YZYP 306T and YZGP 509, were isolated from the halophyte Suaeda aralocaspica collected from the southern edge of the Gurbantunggut desert, north-west China.
30547854	7	73	dep	strains	829:835	arg1	YZGP					851:854	YZGP 509	851:858	YZGP 509	851:858	The genomic DNA G+C contents of strains YZYP 306T and YZGP 509 were 68.49 and 68.53 mol%, respectively.
30547854	7	73	dep	strains	829:835	arg1	306T					842:845	YZYP 306T	837:845	YZYP 306T	837:845	The genomic DNA G+C contents of strains YZYP 306T and YZGP 509 were 68.49 and 68.53 mol%, respectively.
30547854	7	73	dep	strains	829:835	arg1	strains					829:835	strains YZYP 306T and YZGP 509	829:858	strains YZYP 306T and YZGP 509	829:858	The genomic DNA G+C contents of strains YZYP 306T and YZGP 509 were 68.49 and 68.53 mol%, respectively.
30547854	10	74	theme	peptidoglycan	1031:1043	arg1	glycolyl					1049:1056	glycolyl	1049:1056	glycolyl	1049:1056	The acyl type of the peptidoglycan was glycolyl.
30547854	10	74	theme	peptidoglycan	1031:1043	arg1	type					1019:1022	The acyl type	1010:1022	The acyl type of the peptidoglycan	1010:1043	The acyl type of the peptidoglycan was glycolyl.
30547854	2	75	theme	desert	222:227	arg1	edge					196:199	the southern edge	183:199	the southern edge of the Gurbantunggut desert	183:227	Two bacterial strains, YZYP 306T and YZGP 509, were isolated from the halophyte Suaeda aralocaspica collected from the southern edge of the Gurbantunggut desert, north-west China.
30547854	13	76	theme	unidentified	1261:1272	arg1	phospholipid					1274:1285	an unidentified phospholipid	1258:1285	an unidentified phospholipid	1258:1285	The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid and an unidentified glycolipid.
30547854	13	77	theme	polar	1194:1198	arg1	lipids					1200:1205	The polar lipids	1190:1205	The polar lipids	1190:1205	The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid and an unidentified glycolipid.
30547854	13	77	theme	polar	1194:1198	arg1	diphosphatidylglycerol					1212:1233	diphosphatidylglycerol	1212:1233	diphosphatidylglycerol	1212:1233	The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid and an unidentified glycolipid.
29894834	5	0	theme	substitution	765:776	arg1	degree					778:783	fatty acid substitution degree	754:783	fatty acid substitution degree of the chitosan derivative	754:810	The surface hydrophobicity of the nanoparticles increased with the increases of fatty acid hydrophobicity and also fatty acid substitution degree of the chitosan derivative.
29894834	6	1	theme	better	941:946	arg1	efficacy					961:968	better antidiabetic efficacy	941:968	better antidiabetic efficacy	941:968	The nanoparticles with higher surface hydrophobicity displayed higher hepatocyte absorption, more accumulation in the liver and better antidiabetic efficacy.
29894834	7	2	theme	oleic	1147:1151	arg1	acid					1153:1156	oleic acid	1147:1156	oleic acid	1147:1156	Compared with free insulin treatment group, the relative pharmacological availabilities were 233% and 311% for the groups treated with the nanoparticles having lauric acid and oleic acid, respectively, after subcutaneous injection into diabetic mice.
29894834	5	3	theme	surface	643:649	arg1	hydrophobicity					651:664	The surface hydrophobicity	639:664	The surface hydrophobicity of the nanoparticles	639:685	The surface hydrophobicity of the nanoparticles increased with the increases of fatty acid hydrophobicity and also fatty acid substitution degree of the chitosan derivative.
29894834	7	4	theme	treatment	998:1006	arg1	group					1008:1012	free insulin treatment group	985:1012	free insulin treatment group	985:1012	Compared with free insulin treatment group, the relative pharmacological availabilities were 233% and 311% for the groups treated with the nanoparticles having lauric acid and oleic acid, respectively, after subcutaneous injection into diabetic mice.
29894834	4	5	theme	loading	572:578	arg1	capacity					580:587	loading capacity	572:587	loading capacity of the chitosan derivatives	572:615	Both of insulin loading efficiency and loading capacity of the chitosan derivatives were higher than 98%.
29894834	5	6	theme	acid	725:728	arg1	hydrophobicity					730:743	fatty acid hydrophobicity	719:743	fatty acid hydrophobicity	719:743	The surface hydrophobicity of the nanoparticles increased with the increases of fatty acid hydrophobicity and also fatty acid substitution degree of the chitosan derivative.
29894834	2	7	theme	modified	280:287	arg1	carriers					327:334	the carriers	323:334	the carriers	323:334	In this study, lauric acid and oleic acid were respectively conjugated to quaternized chitosan, and the fatty acid modified chitosan derivatives were used as the carriers to deliver the loaded insulin to liver.
29894834	2	7	theme	modified	280:287	arg1	derivatives					298:308	the fatty acid modified chitosan derivatives	265:308	the fatty acid modified chitosan derivatives	265:308	In this study, lauric acid and oleic acid were respectively conjugated to quaternized chitosan, and the fatty acid modified chitosan derivatives were used as the carriers to deliver the loaded insulin to liver.
29894834	5	8	theme	hydrophobicity	730:743	arg1	increases					706:714	the increases	702:714	the increases of fatty acid hydrophobicity	702:743	The surface hydrophobicity of the nanoparticles increased with the increases of fatty acid hydrophobicity and also fatty acid substitution degree of the chitosan derivative.
29894834	2	9	theme	fatty	269:273	arg1	acid					275:278	the fatty acid	265:278	the fatty acid	265:278	In this study, lauric acid and oleic acid were respectively conjugated to quaternized chitosan, and the fatty acid modified chitosan derivatives were used as the carriers to deliver the loaded insulin to liver.
29894834	7	10	theme	free	985:988	arg1	group					1008:1012	free insulin treatment group	985:1012	free insulin treatment group	985:1012	Compared with free insulin treatment group, the relative pharmacological availabilities were 233% and 311% for the groups treated with the nanoparticles having lauric acid and oleic acid, respectively, after subcutaneous injection into diabetic mice.
29894834	3	11	with	nanoparticles	380:392	arg1	sizes					399:403	sizes	399:403	sizes of about 280 nm	399:419	The nanoparticles with sizes of about 280 nm were fabricated via the electrostatic and hydrophobic interactions between insulin and the chitosan derivative.
29894834	3	12	theme	hydrophobic	463:473	arg1	interactions					475:486	the electrostatic and hydrophobic interactions	441:486	the electrostatic and hydrophobic interactions between insulin and the chitosan derivative	441:530	The nanoparticles with sizes of about 280 nm were fabricated via the electrostatic and hydrophobic interactions between insulin and the chitosan derivative.
29894834	7	13	theme	insulin	990:996	arg1	group					1008:1012	free insulin treatment group	985:1012	free insulin treatment group	985:1012	Compared with free insulin treatment group, the relative pharmacological availabilities were 233% and 311% for the groups treated with the nanoparticles having lauric acid and oleic acid, respectively, after subcutaneous injection into diabetic mice.
29894834	3	14	theme	280 nm	414:419	arg1	sizes					399:403	sizes	399:403	sizes of about 280 nm	399:419	The nanoparticles with sizes of about 280 nm were fabricated via the electrostatic and hydrophobic interactions between insulin and the chitosan derivative.
29894834	5	15	theme	derivative	801:810	arg1	degree					778:783	fatty acid substitution degree	754:783	fatty acid substitution degree of the chitosan derivative	754:810	The surface hydrophobicity of the nanoparticles increased with the increases of fatty acid hydrophobicity and also fatty acid substitution degree of the chitosan derivative.
29894834	7	16	theme	lauric	1131:1136	arg1	acid					1138:1141	lauric acid	1131:1141	lauric acid	1131:1141	Compared with free insulin treatment group, the relative pharmacological availabilities were 233% and 311% for the groups treated with the nanoparticles having lauric acid and oleic acid, respectively, after subcutaneous injection into diabetic mice.
29894834	2	17	used	used	315:318	arg2	derivatives					298:308	the fatty acid modified chitosan derivatives	265:308	the fatty acid modified chitosan derivatives	265:308	In this study, lauric acid and oleic acid were respectively conjugated to quaternized chitosan, and the fatty acid modified chitosan derivatives were used as the carriers to deliver the loaded insulin to liver.
29894834	2	17	used	used	315:318	arg2	carriers					327:334	the carriers	323:334	the carriers	323:334	In this study, lauric acid and oleic acid were respectively conjugated to quaternized chitosan, and the fatty acid modified chitosan derivatives were used as the carriers to deliver the loaded insulin to liver.
29894834	7	18	theme	diabetic	1207:1214	arg1	mice					1216:1219	diabetic mice	1207:1219	diabetic mice	1207:1219	Compared with free insulin treatment group, the relative pharmacological availabilities were 233% and 311% for the groups treated with the nanoparticles having lauric acid and oleic acid, respectively, after subcutaneous injection into diabetic mice.
29894834	6	19	theme	more	906:909	arg1	accumulation					911:922	more accumulation	906:922	more accumulation in the liver	906:935	The nanoparticles with higher surface hydrophobicity displayed higher hepatocyte absorption, more accumulation in the liver and better antidiabetic efficacy.
29894834	5	20	theme	fatty	754:758	arg1	acid					760:763	fatty acid	754:763	fatty acid substitution degree of the chitosan derivative	754:810	The surface hydrophobicity of the nanoparticles increased with the increases of fatty acid hydrophobicity and also fatty acid substitution degree of the chitosan derivative.
29894834	6	21	from	efficacy	961:968	arg1	liver					931:935	the liver	927:935	the liver	927:935	The nanoparticles with higher surface hydrophobicity displayed higher hepatocyte absorption, more accumulation in the liver and better antidiabetic efficacy.
29894834	8	22	theme	acid	1291:1294	arg1	polymer					1305:1311	fatty acid modified polymer	1285:1311	fatty acid modified polymer	1285:1311	This study demonstrates that the nanoparticles fabricated from fatty acid modified polymer are an effective liver-targeted delivery system of insulin.
29894834	0	23	theme	Fatty	0:4	arg1	acid					6:9	Fatty acid	0:9	Fatty acid	0:9	Fatty acid and quaternary ammonium modified chitosan nanoparticles for insulin delivery.
29894834	4	24	theme	loading	549:555	arg1	efficiency					557:566	insulin loading efficiency	541:566	insulin loading efficiency	541:566	Both of insulin loading efficiency and loading capacity of the chitosan derivatives were higher than 98%.
29894834	6	25	theme	higher	836:841	arg1	hydrophobicity					851:864	higher surface hydrophobicity	836:864	higher surface hydrophobicity	836:864	The nanoparticles with higher surface hydrophobicity displayed higher hepatocyte absorption, more accumulation in the liver and better antidiabetic efficacy.
29894834	8	26	theme	modified	1296:1303	arg1	polymer					1305:1311	fatty acid modified polymer	1285:1311	fatty acid modified polymer	1285:1311	This study demonstrates that the nanoparticles fabricated from fatty acid modified polymer are an effective liver-targeted delivery system of insulin.
29894834	8	27	theme	fatty	1285:1289	arg1	acid					1291:1294	fatty acid	1285:1294	fatty acid modified polymer	1285:1311	This study demonstrates that the nanoparticles fabricated from fatty acid modified polymer are an effective liver-targeted delivery system of insulin.
29894834	0	28	theme	quaternary	15:24	arg1	ammonium					26:33	quaternary ammonium	15:33	quaternary ammonium	15:33	Fatty acid and quaternary ammonium modified chitosan nanoparticles for insulin delivery.
29894834	4	29	theme	insulin	541:547	arg1	efficiency					557:566	insulin loading efficiency	541:566	insulin loading efficiency	541:566	Both of insulin loading efficiency and loading capacity of the chitosan derivatives were higher than 98%.
29894834	2	30	theme	chitosan	289:296	arg1	carriers					327:334	the carriers	323:334	the carriers	323:334	In this study, lauric acid and oleic acid were respectively conjugated to quaternized chitosan, and the fatty acid modified chitosan derivatives were used as the carriers to deliver the loaded insulin to liver.
29894834	2	30	theme	chitosan	289:296	arg1	derivatives					298:308	the fatty acid modified chitosan derivatives	265:308	the fatty acid modified chitosan derivatives	265:308	In this study, lauric acid and oleic acid were respectively conjugated to quaternized chitosan, and the fatty acid modified chitosan derivatives were used as the carriers to deliver the loaded insulin to liver.
29894834	6	31	from	absorption	894:903	arg1	liver					931:935	the liver	927:935	the liver	927:935	The nanoparticles with higher surface hydrophobicity displayed higher hepatocyte absorption, more accumulation in the liver and better antidiabetic efficacy.
29894834	1	32	theme	major	102:106	arg1	organ					108:112	the major organ	98:112	the major organ where insulin performs its physiological function	98:162	Liver is the major organ where insulin performs its physiological function.
29894834	1	32	theme	major	102:106	arg1	Liver					89:93	Liver	89:93	Liver	89:93	Liver is the major organ where insulin performs its physiological function.
29894834	3	33	theme	electrostatic	445:457	arg1	interactions					475:486	the electrostatic and hydrophobic interactions	441:486	the electrostatic and hydrophobic interactions between insulin and the chitosan derivative	441:530	The nanoparticles with sizes of about 280 nm were fabricated via the electrostatic and hydrophobic interactions between insulin and the chitosan derivative.
29894834	8	34	theme	effective	1320:1328	arg1	system					1354:1359	an effective liver-targeted delivery system	1317:1359	an effective liver-targeted delivery system of insulin	1317:1370	This study demonstrates that the nanoparticles fabricated from fatty acid modified polymer are an effective liver-targeted delivery system of insulin.
29894834	8	34	theme	effective	1320:1328	arg1	nanoparticles					1255:1267	the nanoparticles	1251:1267	the nanoparticles fabricated from fatty acid modified polymer	1251:1311	This study demonstrates that the nanoparticles fabricated from fatty acid modified polymer are an effective liver-targeted delivery system of insulin.
29894834	5	35	theme	fatty	719:723	arg1	hydrophobicity					730:743	fatty acid hydrophobicity	719:743	fatty acid hydrophobicity	719:743	The surface hydrophobicity of the nanoparticles increased with the increases of fatty acid hydrophobicity and also fatty acid substitution degree of the chitosan derivative.
29894834	6	36	theme	hepatocyte	883:892	arg1	absorption					894:903	higher hepatocyte absorption	876:903	higher hepatocyte absorption	876:903	The nanoparticles with higher surface hydrophobicity displayed higher hepatocyte absorption, more accumulation in the liver and better antidiabetic efficacy.
29894834	7	37	theme	subcutaneous	1179:1190	arg1	injection					1192:1200	subcutaneous injection	1179:1200	subcutaneous injection into diabetic mice	1179:1219	Compared with free insulin treatment group, the relative pharmacological availabilities were 233% and 311% for the groups treated with the nanoparticles having lauric acid and oleic acid, respectively, after subcutaneous injection into diabetic mice.
29894834	8	38	theme	delivery	1345:1352	arg1	system					1354:1359	an effective liver-targeted delivery system	1317:1359	an effective liver-targeted delivery system of insulin	1317:1370	This study demonstrates that the nanoparticles fabricated from fatty acid modified polymer are an effective liver-targeted delivery system of insulin.
29894834	8	38	theme	delivery	1345:1352	arg1	nanoparticles					1255:1267	the nanoparticles	1251:1267	the nanoparticles fabricated from fatty acid modified polymer	1251:1311	This study demonstrates that the nanoparticles fabricated from fatty acid modified polymer are an effective liver-targeted delivery system of insulin.
29894834	5	39	theme	chitosan	792:799	arg1	derivative					801:810	the chitosan derivative	788:810	the chitosan derivative	788:810	The surface hydrophobicity of the nanoparticles increased with the increases of fatty acid hydrophobicity and also fatty acid substitution degree of the chitosan derivative.
29894834	6	40	from	accumulation	911:922	arg1	liver					931:935	the liver	927:935	the liver	927:935	The nanoparticles with higher surface hydrophobicity displayed higher hepatocyte absorption, more accumulation in the liver and better antidiabetic efficacy.
29894834	6	41	theme	higher	876:881	arg1	absorption					894:903	higher hepatocyte absorption	876:903	higher hepatocyte absorption	876:903	The nanoparticles with higher surface hydrophobicity displayed higher hepatocyte absorption, more accumulation in the liver and better antidiabetic efficacy.
29894834	8	42	theme	liver-targeted	1330:1343	arg1	system					1354:1359	an effective liver-targeted delivery system	1317:1359	an effective liver-targeted delivery system of insulin	1317:1370	This study demonstrates that the nanoparticles fabricated from fatty acid modified polymer are an effective liver-targeted delivery system of insulin.
29894834	8	42	theme	liver-targeted	1330:1343	arg1	nanoparticles					1255:1267	the nanoparticles	1251:1267	the nanoparticles fabricated from fatty acid modified polymer	1251:1311	This study demonstrates that the nanoparticles fabricated from fatty acid modified polymer are an effective liver-targeted delivery system of insulin.
29894834	0	43	theme	chitosan	44:51	arg1	nanoparticles					53:65	chitosan nanoparticles	44:65	chitosan nanoparticles for insulin delivery	44:86	Fatty acid and quaternary ammonium modified chitosan nanoparticles for insulin delivery.
29894834	5	44	theme	nanoparticles	673:685	arg1	hydrophobicity					651:664	The surface hydrophobicity	639:664	The surface hydrophobicity of the nanoparticles	639:685	The surface hydrophobicity of the nanoparticles increased with the increases of fatty acid hydrophobicity and also fatty acid substitution degree of the chitosan derivative.
29894834	2	45	theme	oleic	196:200	arg1	acid					202:205	oleic acid	196:205	oleic acid	196:205	In this study, lauric acid and oleic acid were respectively conjugated to quaternized chitosan, and the fatty acid modified chitosan derivatives were used as the carriers to deliver the loaded insulin to liver.
29894834	2	46	dep	modified	280:287	arg1	acid					275:278	the fatty acid	265:278	the fatty acid	265:278	In this study, lauric acid and oleic acid were respectively conjugated to quaternized chitosan, and the fatty acid modified chitosan derivatives were used as the carriers to deliver the loaded insulin to liver.
29894834	4	47	theme	derivatives	605:615	arg1	efficiency					557:566	insulin loading efficiency	541:566	insulin loading efficiency	541:566	Both of insulin loading efficiency and loading capacity of the chitosan derivatives were higher than 98%.
29894834	4	47	theme	derivatives	605:615	arg1	capacity					580:587	loading capacity	572:587	loading capacity of the chitosan derivatives	572:615	Both of insulin loading efficiency and loading capacity of the chitosan derivatives were higher than 98%.
29894834	6	48	theme	surface	843:849	arg1	hydrophobicity					851:864	higher surface hydrophobicity	836:864	higher surface hydrophobicity	836:864	The nanoparticles with higher surface hydrophobicity displayed higher hepatocyte absorption, more accumulation in the liver and better antidiabetic efficacy.
29894834	8	49	theme	insulin	1364:1370	arg1	system					1354:1359	an effective liver-targeted delivery system	1317:1359	an effective liver-targeted delivery system of insulin	1317:1370	This study demonstrates that the nanoparticles fabricated from fatty acid modified polymer are an effective liver-targeted delivery system of insulin.
29894834	8	49	theme	insulin	1364:1370	arg1	nanoparticles					1255:1267	the nanoparticles	1251:1267	the nanoparticles fabricated from fatty acid modified polymer	1251:1311	This study demonstrates that the nanoparticles fabricated from fatty acid modified polymer are an effective liver-targeted delivery system of insulin.
29894834	7	50	theme	relative	1019:1026	arg1	availabilities					1044:1057	the relative pharmacological availabilities	1015:1057	the relative pharmacological availabilities	1015:1057	Compared with free insulin treatment group, the relative pharmacological availabilities were 233% and 311% for the groups treated with the nanoparticles having lauric acid and oleic acid, respectively, after subcutaneous injection into diabetic mice.
29894834	7	50	theme	relative	1019:1026	arg1	%					1067:1067	233%	1064:1067	233%	1064:1067	Compared with free insulin treatment group, the relative pharmacological availabilities were 233% and 311% for the groups treated with the nanoparticles having lauric acid and oleic acid, respectively, after subcutaneous injection into diabetic mice.
29894834	1	51	theme	physiological	141:153	arg1	function					155:162	its physiological function	137:162	its physiological function	137:162	Liver is the major organ where insulin performs its physiological function.
29894834	4	52	theme	chitosan	596:603	arg1	derivatives					605:615	the chitosan derivatives	592:615	the chitosan derivatives	592:615	Both of insulin loading efficiency and loading capacity of the chitosan derivatives were higher than 98%.
29894834	0	53	theme	insulin	71:77	arg1	delivery					79:86	insulin delivery	71:86	insulin delivery	71:86	Fatty acid and quaternary ammonium modified chitosan nanoparticles for insulin delivery.
29894834	2	54	theme	lauric	180:185	arg1	acid					187:190	lauric acid	180:190	lauric acid	180:190	In this study, lauric acid and oleic acid were respectively conjugated to quaternized chitosan, and the fatty acid modified chitosan derivatives were used as the carriers to deliver the loaded insulin to liver.
29894834	2	55	theme	quaternized	239:249	arg1	chitosan					251:258	quaternized chitosan	239:258	quaternized chitosan	239:258	In this study, lauric acid and oleic acid were respectively conjugated to quaternized chitosan, and the fatty acid modified chitosan derivatives were used as the carriers to deliver the loaded insulin to liver.
29894834	7	56	theme	pharmacological	1028:1042	arg1	availabilities					1044:1057	the relative pharmacological availabilities	1015:1057	the relative pharmacological availabilities	1015:1057	Compared with free insulin treatment group, the relative pharmacological availabilities were 233% and 311% for the groups treated with the nanoparticles having lauric acid and oleic acid, respectively, after subcutaneous injection into diabetic mice.
29894834	7	56	theme	pharmacological	1028:1042	arg1	%					1067:1067	233%	1064:1067	233%	1064:1067	Compared with free insulin treatment group, the relative pharmacological availabilities were 233% and 311% for the groups treated with the nanoparticles having lauric acid and oleic acid, respectively, after subcutaneous injection into diabetic mice.
29894834	2	57	theme	loaded	351:356	arg1	insulin					358:364	the loaded insulin	347:364	the loaded insulin	347:364	In this study, lauric acid and oleic acid were respectively conjugated to quaternized chitosan, and the fatty acid modified chitosan derivatives were used as the carriers to deliver the loaded insulin to liver.
29894834	7	58	contain	having	1124:1129	arg2	acid					1153:1156	oleic acid	1147:1156	oleic acid	1147:1156	Compared with free insulin treatment group, the relative pharmacological availabilities were 233% and 311% for the groups treated with the nanoparticles having lauric acid and oleic acid, respectively, after subcutaneous injection into diabetic mice.
29894834	7	58	contain	having	1124:1129	arg1	nanoparticles					1110:1122	the nanoparticles	1106:1122	the nanoparticles having lauric acid and oleic acid	1106:1156	Compared with free insulin treatment group, the relative pharmacological availabilities were 233% and 311% for the groups treated with the nanoparticles having lauric acid and oleic acid, respectively, after subcutaneous injection into diabetic mice.
29894834	7	58	contain	having	1124:1129	arg2	acid					1138:1141	lauric acid	1131:1141	lauric acid	1131:1141	Compared with free insulin treatment group, the relative pharmacological availabilities were 233% and 311% for the groups treated with the nanoparticles having lauric acid and oleic acid, respectively, after subcutaneous injection into diabetic mice.
29894834	5	59	theme	acid	760:763	arg1	degree					778:783	fatty acid substitution degree	754:783	fatty acid substitution degree of the chitosan derivative	754:810	The surface hydrophobicity of the nanoparticles increased with the increases of fatty acid hydrophobicity and also fatty acid substitution degree of the chitosan derivative.
29894834	6	60	theme	antidiabetic	948:959	arg1	efficacy					961:968	better antidiabetic efficacy	941:968	better antidiabetic efficacy	941:968	The nanoparticles with higher surface hydrophobicity displayed higher hepatocyte absorption, more accumulation in the liver and better antidiabetic efficacy.
29894834	6	61	with	nanoparticles	817:829	arg1	hydrophobicity					851:864	higher surface hydrophobicity	836:864	higher surface hydrophobicity	836:864	The nanoparticles with higher surface hydrophobicity displayed higher hepatocyte absorption, more accumulation in the liver and better antidiabetic efficacy.
29894834	3	62	theme	chitosan	512:519	arg1	derivative					521:530	the chitosan derivative	508:530	the chitosan derivative	508:530	The nanoparticles with sizes of about 280 nm were fabricated via the electrostatic and hydrophobic interactions between insulin and the chitosan derivative.
30602571	0	0	theme	MAPK	93:96	arg1	signaling					98:106	MAPK signaling	93:106	MAPK signaling	93:106	An immune-stimulating proteoglycan from the medicinal mushroom Huaier up-regulates NF-κB and MAPK signaling via Toll-like receptor 4.
30602571	4	1	theme	∼5.59	473:477	arg1	Da					485:486	∼5.59 × 104 Da	473:486	∼5.59 × 104 Da from Huaier aqueous extract	473:514	Here, we isolated a proteoglycan with a molecular mass of ∼5.59 × 104 Da from Huaier aqueous extract.
30602571	11	2	theme	cancer	1800:1805	arg1	therapy					1807:1813	cancer therapy	1800:1813	cancer therapy	1800:1813	This study has identified the TPG-1 proteoglycan as an antitumor agent and provided insights into TPG-1's molecular mechanism, suggesting a potential utility for applying this agent in cancer therapy.
30602571	2	3	from	efficacy	274:281	arg1	management					293:302	cancer management	286:302	cancer management	286:302	(Huaier) is a mushroom with a long history of use as a medicinal ingredient in China and exhibits good clinical efficacy in cancer management.
30602571	4	4	theme	molecular	455:463	arg1	mass					465:468	a molecular mass	453:468	a molecular mass of ∼5.59 × 104 Da from Huaier aqueous extract	453:514	Here, we isolated a proteoglycan with a molecular mass of ∼5.59 × 104 Da from Huaier aqueous extract.
30602571	6	5	theme	necrosis	861:868	arg1	TNFα					880:883	TNFα	880:883	TNFα	880:883	Using biochemical assays and immunoblotting, we found that exposing murine RAW264.7 macrophages to TPG-1 promotes the production of nitric oxide (NO), tumor necrosis factor α (TNFα), and interleukin-6 (IL-6) through Toll-like receptor 4 (TLR4)-dependent activation of NF-κB and mitogen-activated protein kinase (MAPK) signaling.
30602571	6	5	theme	necrosis	861:868	arg1	factor					870:875	tumor necrosis factor α	855:877	tumor necrosis factor α (TNFα)	855:884	Using biochemical assays and immunoblotting, we found that exposing murine RAW264.7 macrophages to TPG-1 promotes the production of nitric oxide (NO), tumor necrosis factor α (TNFα), and interleukin-6 (IL-6) through Toll-like receptor 4 (TLR4)-dependent activation of NF-κB and mitogen-activated protein kinase (MAPK) signaling.
30602571	4	6	theme	Da	485:486	arg1	mass					465:468	a molecular mass	453:468	a molecular mass of ∼5.59 × 104 Da from Huaier aqueous extract	453:514	Here, we isolated a proteoglycan with a molecular mass of ∼5.59 × 104 Da from Huaier aqueous extract.
30602571	6	7	theme	RAW264.7	779:786	arg1	macrophages					788:798	murine RAW264.7 macrophages	772:798	murine RAW264.7 macrophages	772:798	Using biochemical assays and immunoblotting, we found that exposing murine RAW264.7 macrophages to TPG-1 promotes the production of nitric oxide (NO), tumor necrosis factor α (TNFα), and interleukin-6 (IL-6) through Toll-like receptor 4 (TLR4)-dependent activation of NF-κB and mitogen-activated protein kinase (MAPK) signaling.
30602571	6	8	theme	kinase	1008:1013	arg1	signaling					1022:1030	mitogen-activated protein kinase (MAPK) signaling	982:1030	mitogen-activated protein kinase (MAPK) signaling	982:1030	Using biochemical assays and immunoblotting, we found that exposing murine RAW264.7 macrophages to TPG-1 promotes the production of nitric oxide (NO), tumor necrosis factor α (TNFα), and interleukin-6 (IL-6) through Toll-like receptor 4 (TLR4)-dependent activation of NF-κB and mitogen-activated protein kinase (MAPK) signaling.
30602571	7	9	theme	hepatoma	1113:1120	arg1	cells					1128:1132	human hepatoma HepG2 cells	1107:1132	human hepatoma HepG2 cells	1107:1132	Of note, the TPG-1 treatment significantly inhibited the tumorigenesis of human hepatoma HepG2 cells likely at least in part by increasing serum levels of TNFα and promoting leukocyte infiltration into tumors in nude mice.
30602571	4	10	theme	104	481:483	arg1	Da					485:486	∼5.59 × 104 Da	473:486	∼5.59 × 104 Da from Huaier aqueous extract	473:514	Here, we isolated a proteoglycan with a molecular mass of ∼5.59 × 104 Da from Huaier aqueous extract.
30602571	6	11	theme	murine	772:777	arg1	macrophages					788:798	murine RAW264.7 macrophages	772:798	murine RAW264.7 macrophages	772:798	Using biochemical assays and immunoblotting, we found that exposing murine RAW264.7 macrophages to TPG-1 promotes the production of nitric oxide (NO), tumor necrosis factor α (TNFα), and interleukin-6 (IL-6) through Toll-like receptor 4 (TLR4)-dependent activation of NF-κB and mitogen-activated protein kinase (MAPK) signaling.
30602571	2	12	theme	clinical	265:272	arg1	efficacy					274:281	good clinical efficacy	260:281	good clinical efficacy in cancer management	260:302	(Huaier) is a mushroom with a long history of use as a medicinal ingredient in China and exhibits good clinical efficacy in cancer management.
30602571	9	13	theme	TLR4-NF-κB/MAPK	1502:1516	arg1	cassette					1528:1535	the TLR4-NF-κB/MAPK signaling cassette	1498:1535	the TLR4-NF-κB/MAPK signaling cassette	1498:1535	We conclude that TPG-1 exerts antitumor activity partially through an immune-potentiating effect due to activation of the TLR4-NF-κB/MAPK signaling cassette.
30602571	2	14	dep	mushroom	176:183	arg1	Huaier					163:168	Huaier	163:168	Huaier	163:168	(Huaier) is a mushroom with a long history of use as a medicinal ingredient in China and exhibits good clinical efficacy in cancer management.
30602571	3	15	theme	Huaier	342:347	arg1	components					328:337	the antitumor components	314:337	the antitumor components of Huaier	314:347	However, the antitumor components of Huaier and the underlying molecular mechanisms remain poorly understood.
30602571	3	15	theme	Huaier	342:347	arg1	mechanisms					378:387	the underlying molecular mechanisms	353:387	the underlying molecular mechanisms	353:387	However, the antitumor components of Huaier and the underlying molecular mechanisms remain poorly understood.
30602571	0	16	from	Huaier	63:68	arg1	proteoglycan					22:33	An immune-stimulating proteoglycan	0:33	An immune-stimulating proteoglycan from the medicinal mushroom Huaier	0:68	An immune-stimulating proteoglycan from the medicinal mushroom Huaier up-regulates NF-κB and MAPK signaling via Toll-like receptor 4.
30602571	5	17	theme	total	626:630	arg1	carbohydrate					632:643	its total carbohydrate	622:643	its total carbohydrate	622:643	We named this proteoglycan TPG-1, and using FTIR and additional biochemical analyses, we determined that its total carbohydrate and protein compositions are 43.9 and 41.2%, respectively.
30602571	5	17	theme	total	626:630	arg1	compositions					657:668	protein compositions	649:668	protein compositions	649:668	We named this proteoglycan TPG-1, and using FTIR and additional biochemical analyses, we determined that its total carbohydrate and protein compositions are 43.9 and 41.2%, respectively.
30602571	5	17	theme	total	626:630	arg1	%					687:687	43.9 and 41.2%	674:687	43.9 and 41.2%	674:687	We named this proteoglycan TPG-1, and using FTIR and additional biochemical analyses, we determined that its total carbohydrate and protein compositions are 43.9 and 41.2%, respectively.
30602571	7	18	theme	TPG-1	1046:1050	arg1	treatment					1052:1060	the TPG-1 treatment	1042:1060	the TPG-1 treatment	1042:1060	Of note, the TPG-1 treatment significantly inhibited the tumorigenesis of human hepatoma HepG2 cells likely at least in part by increasing serum levels of TNFα and promoting leukocyte infiltration into tumors in nude mice.
30602571	2	19	theme	good	260:263	arg1	efficacy					274:281	good clinical efficacy	260:281	good clinical efficacy in cancer management	260:302	(Huaier) is a mushroom with a long history of use as a medicinal ingredient in China and exhibits good clinical efficacy in cancer management.
30602571	9	20	theme	antitumor	1410:1418	arg1	activity					1420:1427	antitumor activity	1410:1427	antitumor activity	1410:1427	We conclude that TPG-1 exerts antitumor activity partially through an immune-potentiating effect due to activation of the TLR4-NF-κB/MAPK signaling cassette.
30602571	3	21	theme	antitumor	318:326	arg1	components					328:337	the antitumor components	314:337	the antitumor components of Huaier	314:347	However, the antitumor components of Huaier and the underlying molecular mechanisms remain poorly understood.
30602571	5	22	theme	biochemical	581:591	arg1	analyses					593:600	additional biochemical analyses	570:600	additional biochemical analyses	570:600	We named this proteoglycan TPG-1, and using FTIR and additional biochemical analyses, we determined that its total carbohydrate and protein compositions are 43.9 and 41.2%, respectively.
30602571	5	23	theme	additional	570:579	arg1	analyses					593:600	additional biochemical analyses	570:600	additional biochemical analyses	570:600	We named this proteoglycan TPG-1, and using FTIR and additional biochemical analyses, we determined that its total carbohydrate and protein compositions are 43.9 and 41.2%, respectively.
30602571	9	24	theme	due	1477:1479	arg1	effect					1470:1475	an immune-potentiating effect	1447:1475	an immune-potentiating effect due to activation of the TLR4-NF-κB/MAPK signaling cassette	1447:1535	We conclude that TPG-1 exerts antitumor activity partially through an immune-potentiating effect due to activation of the TLR4-NF-κB/MAPK signaling cassette.
30602571	10	25	theme	candidate	1574:1582	arg1	TPG-1					1549:1553	TPG-1	1549:1553	TPG-1	1549:1553	Therefore, TPG-1 may be a promising candidate drug for cancer immunotherapy.
30602571	10	25	theme	candidate	1574:1582	arg1	drug					1584:1587	a promising candidate drug	1562:1587	a promising candidate drug for cancer immunotherapy	1562:1612	Therefore, TPG-1 may be a promising candidate drug for cancer immunotherapy.
30602571	6	26	theme	protein	1000:1006	arg1	MAPK					1016:1019	MAPK	1016:1019	MAPK	1016:1019	Using biochemical assays and immunoblotting, we found that exposing murine RAW264.7 macrophages to TPG-1 promotes the production of nitric oxide (NO), tumor necrosis factor α (TNFα), and interleukin-6 (IL-6) through Toll-like receptor 4 (TLR4)-dependent activation of NF-κB and mitogen-activated protein kinase (MAPK) signaling.
30602571	6	26	theme	protein	1000:1006	arg1	kinase					1008:1013	mitogen-activated protein kinase	982:1013	mitogen-activated protein kinase (MAPK) signaling	982:1030	Using biochemical assays and immunoblotting, we found that exposing murine RAW264.7 macrophages to TPG-1 promotes the production of nitric oxide (NO), tumor necrosis factor α (TNFα), and interleukin-6 (IL-6) through Toll-like receptor 4 (TLR4)-dependent activation of NF-κB and mitogen-activated protein kinase (MAPK) signaling.
30602571	0	27	theme	Toll-like	112:120	arg1	receptor					122:129	Toll-like receptor 4	112:131	Toll-like receptor 4	112:131	An immune-stimulating proteoglycan from the medicinal mushroom Huaier up-regulates NF-κB and MAPK signaling via Toll-like receptor 4.
30602571	10	28	theme	cancer	1593:1598	arg1	immunotherapy					1600:1612	cancer immunotherapy	1593:1612	cancer immunotherapy	1593:1612	Therefore, TPG-1 may be a promising candidate drug for cancer immunotherapy.
30602571	4	29	from	extract	508:514	arg1	mass					465:468	a molecular mass	453:468	a molecular mass of ∼5.59 × 104 Da from Huaier aqueous extract	453:514	Here, we isolated a proteoglycan with a molecular mass of ∼5.59 × 104 Da from Huaier aqueous extract.
30602571	4	29	from	extract	508:514	arg1	Da					485:486	∼5.59 × 104 Da	473:486	∼5.59 × 104 Da from Huaier aqueous extract	473:514	Here, we isolated a proteoglycan with a molecular mass of ∼5.59 × 104 Da from Huaier aqueous extract.
30602571	5	30	theme	proteoglycan	531:542	arg1	TPG-1					544:548	this proteoglycan TPG-1	526:548	this proteoglycan TPG-1	526:548	We named this proteoglycan TPG-1, and using FTIR and additional biochemical analyses, we determined that its total carbohydrate and protein compositions are 43.9 and 41.2%, respectively.
30602571	4	31	with	proteoglycan	435:446	arg1	mass					465:468	a molecular mass	453:468	a molecular mass of ∼5.59 × 104 Da from Huaier aqueous extract	453:514	Here, we isolated a proteoglycan with a molecular mass of ∼5.59 × 104 Da from Huaier aqueous extract.
30602571	0	32	theme	immune-stimulating	3:20	arg1	proteoglycan					22:33	An immune-stimulating proteoglycan	0:33	An immune-stimulating proteoglycan from the medicinal mushroom Huaier	0:68	An immune-stimulating proteoglycan from the medicinal mushroom Huaier up-regulates NF-κB and MAPK signaling via Toll-like receptor 4.
30602571	6	33	theme	oxide	843:847	arg1	production					822:831	the production	818:831	the production of nitric oxide (NO), tumor necrosis factor α (TNFα), and interleukin-6 (IL-6)	818:910	Using biochemical assays and immunoblotting, we found that exposing murine RAW264.7 macrophages to TPG-1 promotes the production of nitric oxide (NO), tumor necrosis factor α (TNFα), and interleukin-6 (IL-6) through Toll-like receptor 4 (TLR4)-dependent activation of NF-κB and mitogen-activated protein kinase (MAPK) signaling.
30602571	8	34	theme	good	1277:1280	arg1	activity					1292:1299	good antitumor activity	1277:1299	good antitumor activity	1277:1299	TPG-1 also exhibited good antitumor activity in hepatoma H22-bearing mice and had no obvious adverse effects in these mice.
30602571	10	35	theme	promising	1564:1572	arg1	TPG-1					1549:1553	TPG-1	1549:1553	TPG-1	1549:1553	Therefore, TPG-1 may be a promising candidate drug for cancer immunotherapy.
30602571	10	35	theme	promising	1564:1572	arg1	drug					1584:1587	a promising candidate drug	1562:1587	a promising candidate drug for cancer immunotherapy	1562:1612	Therefore, TPG-1 may be a promising candidate drug for cancer immunotherapy.
30602571	3	36	theme	molecular	368:376	arg1	mechanisms					378:387	the underlying molecular mechanisms	353:387	the underlying molecular mechanisms	353:387	However, the antitumor components of Huaier and the underlying molecular mechanisms remain poorly understood.
30602571	2	37	theme	cancer	286:291	arg1	management					293:302	cancer management	286:302	cancer management	286:302	(Huaier) is a mushroom with a long history of use as a medicinal ingredient in China and exhibits good clinical efficacy in cancer management.
30602571	2	38	from	ingredient	227:236	arg1	China					241:245	China	241:245	China	241:245	(Huaier) is a mushroom with a long history of use as a medicinal ingredient in China and exhibits good clinical efficacy in cancer management.
30602571	6	39	theme	NF-κB	972:976	arg1	activation					958:967	Toll-like receptor 4 (TLR4)-dependent activation	920:967	Toll-like receptor 4 (TLR4)-dependent activation of NF-κB	920:976	Using biochemical assays and immunoblotting, we found that exposing murine RAW264.7 macrophages to TPG-1 promotes the production of nitric oxide (NO), tumor necrosis factor α (TNFα), and interleukin-6 (IL-6) through Toll-like receptor 4 (TLR4)-dependent activation of NF-κB and mitogen-activated protein kinase (MAPK) signaling.
30602571	6	39	theme	NF-κB	972:976	arg1	signaling					1022:1030	mitogen-activated protein kinase (MAPK) signaling	982:1030	mitogen-activated protein kinase (MAPK) signaling	982:1030	Using biochemical assays and immunoblotting, we found that exposing murine RAW264.7 macrophages to TPG-1 promotes the production of nitric oxide (NO), tumor necrosis factor α (TNFα), and interleukin-6 (IL-6) through Toll-like receptor 4 (TLR4)-dependent activation of NF-κB and mitogen-activated protein kinase (MAPK) signaling.
30602571	6	40	theme	interleukin-6	891:903	arg1	production					822:831	the production	818:831	the production of nitric oxide (NO), tumor necrosis factor α (TNFα), and interleukin-6 (IL-6)	818:910	Using biochemical assays and immunoblotting, we found that exposing murine RAW264.7 macrophages to TPG-1 promotes the production of nitric oxide (NO), tumor necrosis factor α (TNFα), and interleukin-6 (IL-6) through Toll-like receptor 4 (TLR4)-dependent activation of NF-κB and mitogen-activated protein kinase (MAPK) signaling.
30602571	8	41	theme	H22-bearing	1313:1323	arg1	mice					1325:1328	hepatoma H22-bearing mice	1304:1328	hepatoma H22-bearing mice	1304:1328	TPG-1 also exhibited good antitumor activity in hepatoma H22-bearing mice and had no obvious adverse effects in these mice.
30602571	9	42	theme	signaling	1518:1526	arg1	cassette					1528:1535	the TLR4-NF-κB/MAPK signaling cassette	1498:1535	the TLR4-NF-κB/MAPK signaling cassette	1498:1535	We conclude that TPG-1 exerts antitumor activity partially through an immune-potentiating effect due to activation of the TLR4-NF-κB/MAPK signaling cassette.
30602571	7	43	theme	leukocyte	1207:1215	arg1	infiltration					1217:1228	leukocyte infiltration	1207:1228	leukocyte infiltration into tumors in nude mice	1207:1253	Of note, the TPG-1 treatment significantly inhibited the tumorigenesis of human hepatoma HepG2 cells likely at least in part by increasing serum levels of TNFα and promoting leukocyte infiltration into tumors in nude mice.
30602571	8	44	contain	had	1334:1336	arg1	TPG-1					1256:1260	TPG-1	1256:1260	TPG-1	1256:1260	TPG-1 also exhibited good antitumor activity in hepatoma H22-bearing mice and had no obvious adverse effects in these mice.
30602571	8	44	contain	had	1334:1336	arg2	effects					1357:1363	no obvious adverse effects	1338:1363	no obvious adverse effects	1338:1363	TPG-1 also exhibited good antitumor activity in hepatoma H22-bearing mice and had no obvious adverse effects in these mice.
30602571	6	45	theme	nitric	836:841	arg1	NO					850:851	NO	850:851	NO	850:851	Using biochemical assays and immunoblotting, we found that exposing murine RAW264.7 macrophages to TPG-1 promotes the production of nitric oxide (NO), tumor necrosis factor α (TNFα), and interleukin-6 (IL-6) through Toll-like receptor 4 (TLR4)-dependent activation of NF-κB and mitogen-activated protein kinase (MAPK) signaling.
30602571	6	45	theme	nitric	836:841	arg1	oxide					843:847	nitric oxide	836:847	nitric oxide (NO)	836:852	Using biochemical assays and immunoblotting, we found that exposing murine RAW264.7 macrophages to TPG-1 promotes the production of nitric oxide (NO), tumor necrosis factor α (TNFα), and interleukin-6 (IL-6) through Toll-like receptor 4 (TLR4)-dependent activation of NF-κB and mitogen-activated protein kinase (MAPK) signaling.
30602571	8	46	theme	antitumor	1282:1290	arg1	activity					1292:1299	good antitumor activity	1277:1299	good antitumor activity	1277:1299	TPG-1 also exhibited good antitumor activity in hepatoma H22-bearing mice and had no obvious adverse effects in these mice.
30602571	11	47	from	agent	1791:1795	arg1	therapy					1807:1813	cancer therapy	1800:1813	cancer therapy	1800:1813	This study has identified the TPG-1 proteoglycan as an antitumor agent and provided insights into TPG-1's molecular mechanism, suggesting a potential utility for applying this agent in cancer therapy.
30602571	0	48	theme	medicinal	44:52	arg1	Huaier					63:68	the medicinal mushroom Huaier	40:68	the medicinal mushroom Huaier	40:68	An immune-stimulating proteoglycan from the medicinal mushroom Huaier up-regulates NF-κB and MAPK signaling via Toll-like receptor 4.
30602571	11	49	theme	molecular	1721:1729	arg1	mechanism					1731:1739	TPG-1's molecular mechanism	1713:1739	TPG-1's molecular mechanism	1713:1739	This study has identified the TPG-1 proteoglycan as an antitumor agent and provided insights into TPG-1's molecular mechanism, suggesting a potential utility for applying this agent in cancer therapy.
30602571	8	50	theme	adverse	1349:1355	arg1	effects					1357:1363	no obvious adverse effects	1338:1363	no obvious adverse effects	1338:1363	TPG-1 also exhibited good antitumor activity in hepatoma H22-bearing mice and had no obvious adverse effects in these mice.
30602571	8	51	theme	hepatoma	1304:1311	arg1	mice					1325:1328	hepatoma H22-bearing mice	1304:1328	hepatoma H22-bearing mice	1304:1328	TPG-1 also exhibited good antitumor activity in hepatoma H22-bearing mice and had no obvious adverse effects in these mice.
30602571	6	52	theme	-dependent	947:956	arg1	activation					958:967	Toll-like receptor 4 (TLR4)-dependent activation	920:967	Toll-like receptor 4 (TLR4)-dependent activation of NF-κB	920:976	Using biochemical assays and immunoblotting, we found that exposing murine RAW264.7 macrophages to TPG-1 promotes the production of nitric oxide (NO), tumor necrosis factor α (TNFα), and interleukin-6 (IL-6) through Toll-like receptor 4 (TLR4)-dependent activation of NF-κB and mitogen-activated protein kinase (MAPK) signaling.
30602571	7	53	theme	cells	1128:1132	arg1	tumorigenesis					1090:1102	the tumorigenesis	1086:1102	the tumorigenesis of human hepatoma HepG2 cells	1086:1132	Of note, the TPG-1 treatment significantly inhibited the tumorigenesis of human hepatoma HepG2 cells likely at least in part by increasing serum levels of TNFα and promoting leukocyte infiltration into tumors in nude mice.
30602571	4	54	theme	Huaier	493:498	arg1	extract					508:514	Huaier aqueous extract	493:514	Huaier aqueous extract	493:514	Here, we isolated a proteoglycan with a molecular mass of ∼5.59 × 104 Da from Huaier aqueous extract.
30602571	9	55	theme	cassette	1528:1535	arg1	activation					1484:1493	activation	1484:1493	activation of the TLR4-NF-κB/MAPK signaling cassette	1484:1535	We conclude that TPG-1 exerts antitumor activity partially through an immune-potentiating effect due to activation of the TLR4-NF-κB/MAPK signaling cassette.
30602571	7	56	theme	human	1107:1111	arg1	cells					1128:1132	human hepatoma HepG2 cells	1107:1132	human hepatoma HepG2 cells	1107:1132	Of note, the TPG-1 treatment significantly inhibited the tumorigenesis of human hepatoma HepG2 cells likely at least in part by increasing serum levels of TNFα and promoting leukocyte infiltration into tumors in nude mice.
30602571	11	57	theme	potential	1755:1763	arg1	utility					1765:1771	a potential utility	1753:1771	a potential utility for applying this agent in cancer therapy	1753:1813	This study has identified the TPG-1 proteoglycan as an antitumor agent and provided insights into TPG-1's molecular mechanism, suggesting a potential utility for applying this agent in cancer therapy.
30602571	2	58	theme	use	208:210	arg1	history					197:203	a long history	190:203	a long history of use as a medicinal ingredient in China	190:245	(Huaier) is a mushroom with a long history of use as a medicinal ingredient in China and exhibits good clinical efficacy in cancer management.
30602571	0	59	theme	mushroom	54:61	arg1	Huaier					63:68	the medicinal mushroom Huaier	40:68	the medicinal mushroom Huaier	40:68	An immune-stimulating proteoglycan from the medicinal mushroom Huaier up-regulates NF-κB and MAPK signaling via Toll-like receptor 4.
30602571	6	60	theme	factor	870:875	arg1	production					822:831	the production	818:831	the production of nitric oxide (NO), tumor necrosis factor α (TNFα), and interleukin-6 (IL-6)	818:910	Using biochemical assays and immunoblotting, we found that exposing murine RAW264.7 macrophages to TPG-1 promotes the production of nitric oxide (NO), tumor necrosis factor α (TNFα), and interleukin-6 (IL-6) through Toll-like receptor 4 (TLR4)-dependent activation of NF-κB and mitogen-activated protein kinase (MAPK) signaling.
30602571	6	61	theme	tumor	855:859	arg1	TNFα					880:883	TNFα	880:883	TNFα	880:883	Using biochemical assays and immunoblotting, we found that exposing murine RAW264.7 macrophages to TPG-1 promotes the production of nitric oxide (NO), tumor necrosis factor α (TNFα), and interleukin-6 (IL-6) through Toll-like receptor 4 (TLR4)-dependent activation of NF-κB and mitogen-activated protein kinase (MAPK) signaling.
30602571	6	61	theme	tumor	855:859	arg1	factor					870:875	tumor necrosis factor α	855:877	tumor necrosis factor α (TNFα)	855:884	Using biochemical assays and immunoblotting, we found that exposing murine RAW264.7 macrophages to TPG-1 promotes the production of nitric oxide (NO), tumor necrosis factor α (TNFα), and interleukin-6 (IL-6) through Toll-like receptor 4 (TLR4)-dependent activation of NF-κB and mitogen-activated protein kinase (MAPK) signaling.
30602571	4	62	from	mass	465:468	arg1	extract					508:514	Huaier aqueous extract	493:514	Huaier aqueous extract	493:514	Here, we isolated a proteoglycan with a molecular mass of ∼5.59 × 104 Da from Huaier aqueous extract.
30602571	6	63	theme	biochemical	710:720	arg1	assays					722:727	biochemical assays	710:727	biochemical assays	710:727	Using biochemical assays and immunoblotting, we found that exposing murine RAW264.7 macrophages to TPG-1 promotes the production of nitric oxide (NO), tumor necrosis factor α (TNFα), and interleukin-6 (IL-6) through Toll-like receptor 4 (TLR4)-dependent activation of NF-κB and mitogen-activated protein kinase (MAPK) signaling.
30602571	7	64	theme	TNFα	1188:1191	arg1	levels					1178:1183	serum levels	1172:1183	serum levels of TNFα	1172:1191	Of note, the TPG-1 treatment significantly inhibited the tumorigenesis of human hepatoma HepG2 cells likely at least in part by increasing serum levels of TNFα and promoting leukocyte infiltration into tumors in nude mice.
30602571	4	65	theme	aqueous	500:506	arg1	extract					508:514	Huaier aqueous extract	493:514	Huaier aqueous extract	493:514	Here, we isolated a proteoglycan with a molecular mass of ∼5.59 × 104 Da from Huaier aqueous extract.
30602571	5	66	theme	protein	649:655	arg1	%					687:687	43.9 and 41.2%	674:687	43.9 and 41.2%	674:687	We named this proteoglycan TPG-1, and using FTIR and additional biochemical analyses, we determined that its total carbohydrate and protein compositions are 43.9 and 41.2%, respectively.
30602571	5	66	theme	protein	649:655	arg1	compositions					657:668	protein compositions	649:668	protein compositions	649:668	We named this proteoglycan TPG-1, and using FTIR and additional biochemical analyses, we determined that its total carbohydrate and protein compositions are 43.9 and 41.2%, respectively.
30602571	5	66	theme	protein	649:655	arg1	carbohydrate					632:643	its total carbohydrate	622:643	its total carbohydrate	622:643	We named this proteoglycan TPG-1, and using FTIR and additional biochemical analyses, we determined that its total carbohydrate and protein compositions are 43.9 and 41.2%, respectively.
30602571	2	67	theme	long	192:195	arg1	history					197:203	a long history	190:203	a long history of use as a medicinal ingredient in China	190:245	(Huaier) is a mushroom with a long history of use as a medicinal ingredient in China and exhibits good clinical efficacy in cancer management.
30602571	6	68	theme	Toll-like	920:928	arg1	receptor					930:937	Toll-like receptor 4	920:939	Toll-like receptor 4	920:939	Using biochemical assays and immunoblotting, we found that exposing murine RAW264.7 macrophages to TPG-1 promotes the production of nitric oxide (NO), tumor necrosis factor α (TNFα), and interleukin-6 (IL-6) through Toll-like receptor 4 (TLR4)-dependent activation of NF-κB and mitogen-activated protein kinase (MAPK) signaling.
30602571	7	69	theme	nude	1245:1248	arg1	mice					1250:1253	nude mice	1245:1253	nude mice	1245:1253	Of note, the TPG-1 treatment significantly inhibited the tumorigenesis of human hepatoma HepG2 cells likely at least in part by increasing serum levels of TNFα and promoting leukocyte infiltration into tumors in nude mice.
30602571	1	70	theme	Trametes	134:141	arg1	Murr					156:159	Trametes robiniophila Murr	134:159	Trametes robiniophila Murr.	134:160	Trametes robiniophila Murr.
30602571	9	71	theme	immune-potentiating	1450:1468	arg1	effect					1470:1475	an immune-potentiating effect	1447:1475	an immune-potentiating effect due to activation of the TLR4-NF-κB/MAPK signaling cassette	1447:1535	We conclude that TPG-1 exerts antitumor activity partially through an immune-potentiating effect due to activation of the TLR4-NF-κB/MAPK signaling cassette.
30602571	6	72	dep	-dependent	947:956	arg1	receptor					930:937	Toll-like receptor 4	920:939	Toll-like receptor 4	920:939	Using biochemical assays and immunoblotting, we found that exposing murine RAW264.7 macrophages to TPG-1 promotes the production of nitric oxide (NO), tumor necrosis factor α (TNFα), and interleukin-6 (IL-6) through Toll-like receptor 4 (TLR4)-dependent activation of NF-κB and mitogen-activated protein kinase (MAPK) signaling.
30602571	1	73	theme	robiniophila	143:154	arg1	Murr					156:159	Trametes robiniophila Murr	134:159	Trametes robiniophila Murr.	134:160	Trametes robiniophila Murr.
30602571	2	74	with	mushroom	176:183	arg1	history					197:203	a long history	190:203	a long history of use as a medicinal ingredient in China	190:245	(Huaier) is a mushroom with a long history of use as a medicinal ingredient in China and exhibits good clinical efficacy in cancer management.
30602571	11	75	theme	TPG-1	1645:1649	arg1	agent					1680:1684	an antitumor agent	1667:1684	an antitumor agent	1667:1684	This study has identified the TPG-1 proteoglycan as an antitumor agent and provided insights into TPG-1's molecular mechanism, suggesting a potential utility for applying this agent in cancer therapy.
30602571	11	75	theme	TPG-1	1645:1649	arg1	proteoglycan					1651:1662	the TPG-1 proteoglycan	1641:1662	the TPG-1 proteoglycan	1641:1662	This study has identified the TPG-1 proteoglycan as an antitumor agent and provided insights into TPG-1's molecular mechanism, suggesting a potential utility for applying this agent in cancer therapy.
30602571	8	76	theme	obvious	1341:1347	arg1	effects					1357:1363	no obvious adverse effects	1338:1363	no obvious adverse effects	1338:1363	TPG-1 also exhibited good antitumor activity in hepatoma H22-bearing mice and had no obvious adverse effects in these mice.
30602571	7	77	from	tumors	1235:1240	arg1	mice					1250:1253	nude mice	1245:1253	nude mice	1245:1253	Of note, the TPG-1 treatment significantly inhibited the tumorigenesis of human hepatoma HepG2 cells likely at least in part by increasing serum levels of TNFα and promoting leukocyte infiltration into tumors in nude mice.
30602571	11	78	theme	antitumor	1670:1678	arg1	agent					1680:1684	an antitumor agent	1667:1684	an antitumor agent	1667:1684	This study has identified the TPG-1 proteoglycan as an antitumor agent and provided insights into TPG-1's molecular mechanism, suggesting a potential utility for applying this agent in cancer therapy.
30602571	11	78	theme	antitumor	1670:1678	arg1	proteoglycan					1651:1662	the TPG-1 proteoglycan	1641:1662	the TPG-1 proteoglycan	1641:1662	This study has identified the TPG-1 proteoglycan as an antitumor agent and provided insights into TPG-1's molecular mechanism, suggesting a potential utility for applying this agent in cancer therapy.
30602571	6	79	theme	mitogen-activated	982:998	arg1	MAPK					1016:1019	MAPK	1016:1019	MAPK	1016:1019	Using biochemical assays and immunoblotting, we found that exposing murine RAW264.7 macrophages to TPG-1 promotes the production of nitric oxide (NO), tumor necrosis factor α (TNFα), and interleukin-6 (IL-6) through Toll-like receptor 4 (TLR4)-dependent activation of NF-κB and mitogen-activated protein kinase (MAPK) signaling.
30602571	6	79	theme	mitogen-activated	982:998	arg1	kinase					1008:1013	mitogen-activated protein kinase	982:1013	mitogen-activated protein kinase (MAPK) signaling	982:1030	Using biochemical assays and immunoblotting, we found that exposing murine RAW264.7 macrophages to TPG-1 promotes the production of nitric oxide (NO), tumor necrosis factor α (TNFα), and interleukin-6 (IL-6) through Toll-like receptor 4 (TLR4)-dependent activation of NF-κB and mitogen-activated protein kinase (MAPK) signaling.
30602571	4	80	theme	×	479:479	arg1	Da					485:486	∼5.59 × 104 Da	473:486	∼5.59 × 104 Da from Huaier aqueous extract	473:514	Here, we isolated a proteoglycan with a molecular mass of ∼5.59 × 104 Da from Huaier aqueous extract.
30602571	2	81	theme	medicinal	217:225	arg1	ingredient					227:236	a medicinal ingredient	215:236	a medicinal ingredient in China	215:245	(Huaier) is a mushroom with a long history of use as a medicinal ingredient in China and exhibits good clinical efficacy in cancer management.
30602571	7	82	theme	serum	1172:1176	arg1	levels					1178:1183	serum levels	1172:1183	serum levels of TNFα	1172:1191	Of note, the TPG-1 treatment significantly inhibited the tumorigenesis of human hepatoma HepG2 cells likely at least in part by increasing serum levels of TNFα and promoting leukocyte infiltration into tumors in nude mice.
30602571	3	83	theme	underlying	357:366	arg1	mechanisms					378:387	the underlying molecular mechanisms	353:387	the underlying molecular mechanisms	353:387	However, the antitumor components of Huaier and the underlying molecular mechanisms remain poorly understood.
30602571	7	84	theme	HepG2	1122:1126	arg1	cells					1128:1132	human hepatoma HepG2 cells	1107:1132	human hepatoma HepG2 cells	1107:1132	Of note, the TPG-1 treatment significantly inhibited the tumorigenesis of human hepatoma HepG2 cells likely at least in part by increasing serum levels of TNFα and promoting leukocyte infiltration into tumors in nude mice.
30362936	11	0	theme	muramic	1199:1205	arg1	type					1212:1215	The muramic acid type	1195:1215	The muramic acid type of peptidoglycan	1195:1232	The muramic acid type of peptidoglycan was N-glycolyl.
30362936	11	0	theme	muramic	1199:1205	arg1	N-glycolyl					1238:1247	N-glycolyl	1238:1247	N-glycolyl	1238:1247	The muramic acid type of peptidoglycan was N-glycolyl.
30362936	2	1	theme	YJYP	128:131	arg1	strains					119:125	Two actinobacterial strains	99:125	Two actinobacterial strains	99:125	Two actinobacterial strains, YJYP 303T and YZYP 518, were isolated from two species of halophytes collected from the southern edge of the Gurbantunggut Desert.
30362936	2	1	theme	YJYP	128:131	arg1	303T					133:136	YJYP 303T	128:136	YJYP 303T	128:136	Two actinobacterial strains, YJYP 303T and YZYP 518, were isolated from two species of halophytes collected from the southern edge of the Gurbantunggut Desert.
30362936	5	2	theme	genus	569:573	arg1	Microbacterium					575:588	the genus Microbacterium	565:588	the genus Microbacterium	565:588	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the two strains are associated with members of the genus Microbacterium.
30362936	13	3	theme	cell-wall	1356:1364	arg1	acids					1372:1376	The cell-wall amino acids	1352:1376	The cell-wall amino acids	1352:1376	The cell-wall amino acids were glutamic acid, ornithine, glycine and alanine.
30362936	13	3	theme	cell-wall	1356:1364	arg1	acid					1392:1395	glutamic acid	1383:1395	glutamic acid	1383:1395	The cell-wall amino acids were glutamic acid, ornithine, glycine and alanine.
30362936	7	4	theme	average	787:793	arg1	identity					806:813	average nucleotide identity	787:813	The average nucleotide identity values of strain YJYP 303T and YZYP 518 to M. halotolerans YIM 70130T	783:883	The average nucleotide identity values of strain YJYP 303T and YZYP 518 to M. halotolerans YIM 70130T were determined to be 79.97 and 80.03 %, respectively.
30362936	6	5	theme	YIM	681:683	arg1	identity					724:731	97.58 % 16S rRNA gene sequence identity	693:731	97.58 % 16S rRNA gene sequence identity	693:731	In the phylogenetic tree, the two strains shared a clade with Microbacterium halotolerans YIM 70130T (97.58 % 16S rRNA gene sequence identity) and Microbacterium populi KCTC 29152T (96.54 %).
30362936	6	5	theme	YIM	681:683	arg1	70130T					685:690	Microbacterium halotolerans YIM 70130T	653:690	Microbacterium halotolerans YIM 70130T (97.58 % 16S rRNA gene sequence identity)	653:732	In the phylogenetic tree, the two strains shared a clade with Microbacterium halotolerans YIM 70130T (97.58 % 16S rRNA gene sequence identity) and Microbacterium populi KCTC 29152T (96.54 %).
30362936	3	6	dep	Gram-stain-positive	270:288	arg1	aerobic					291:297	aerobic	291:297	aerobic	291:297	Cells were Gram-stain-positive, aerobic, short rods and without flagella.
30362936	3	6	dep	Gram-stain-positive	270:288	arg1	short					300:304	short	300:304	short	300:304	Cells were Gram-stain-positive, aerobic, short rods and without flagella.
30362936	4	7	theme	NaCl	442:445	arg1	presence					413:420	the presence	409:420	the presence of up to 15 % (w/v) NaCl	409:445	Growth of the two strains was found to occur at 4-44 °C, pH 6.0-12.0 and in the presence of up to 15 % (w/v) NaCl.
30362936	5	8	theme	Microbacterium	575:588	arg1	members					554:560	members	554:560	members of the genus Microbacterium	554:588	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the two strains are associated with members of the genus Microbacterium.
30362936	6	9	theme	rRNA	705:708	arg1	70130T					685:690	Microbacterium halotolerans YIM 70130T	653:690	Microbacterium halotolerans YIM 70130T (97.58 % 16S rRNA gene sequence identity)	653:732	In the phylogenetic tree, the two strains shared a clade with Microbacterium halotolerans YIM 70130T (97.58 % 16S rRNA gene sequence identity) and Microbacterium populi KCTC 29152T (96.54 %).
30362936	6	9	theme	rRNA	705:708	arg1	identity					724:731	97.58 % 16S rRNA gene sequence identity	693:731	97.58 % 16S rRNA gene sequence identity	693:731	In the phylogenetic tree, the two strains shared a clade with Microbacterium halotolerans YIM 70130T (97.58 % 16S rRNA gene sequence identity) and Microbacterium populi KCTC 29152T (96.54 %).
30362936	6	10	with	clade	642:646	arg1	identity					724:731	97.58 % 16S rRNA gene sequence identity	693:731	97.58 % 16S rRNA gene sequence identity	693:731	In the phylogenetic tree, the two strains shared a clade with Microbacterium halotolerans YIM 70130T (97.58 % 16S rRNA gene sequence identity) and Microbacterium populi KCTC 29152T (96.54 %).
30362936	6	10	with	clade	642:646	arg1	70130T					685:690	Microbacterium halotolerans YIM 70130T	653:690	Microbacterium halotolerans YIM 70130T (97.58 % 16S rRNA gene sequence identity)	653:732	In the phylogenetic tree, the two strains shared a clade with Microbacterium halotolerans YIM 70130T (97.58 % 16S rRNA gene sequence identity) and Microbacterium populi KCTC 29152T (96.54 %).
30362936	6	10	with	clade	642:646	arg1	29152T					765:770	Microbacterium populi KCTC 29152T	738:770	Microbacterium populi KCTC 29152T (96.54 %)	738:780	In the phylogenetic tree, the two strains shared a clade with Microbacterium halotolerans YIM 70130T (97.58 % 16S rRNA gene sequence identity) and Microbacterium populi KCTC 29152T (96.54 %).
30362936	6	10	with	clade	642:646	arg1	%					779:779	96.54 %	773:779	96.54 %	773:779	In the phylogenetic tree, the two strains shared a clade with Microbacterium halotolerans YIM 70130T (97.58 % 16S rRNA gene sequence identity) and Microbacterium populi KCTC 29152T (96.54 %).
30362936	7	11	theme	YZYP	846:849	arg1	%					923:923	79.97 and 80.03 %	907:923	79.97 and 80.03 %	907:923	The average nucleotide identity values of strain YJYP 303T and YZYP 518 to M. halotolerans YIM 70130T were determined to be 79.97 and 80.03 %, respectively.
30362936	7	11	theme	YZYP	846:849	arg1	values					815:820	The average nucleotide identity values	783:820	The average nucleotide identity values of strain YJYP 303T and YZYP 518 to M. halotolerans YIM 70130T	783:883	The average nucleotide identity values of strain YJYP 303T and YZYP 518 to M. halotolerans YIM 70130T were determined to be 79.97 and 80.03 %, respectively.
30362936	1	12	theme	endophytic	46:55	arg1	actinobacterium					57:71	a novel endophytic actinobacterium	38:71	a novel endophytic actinobacterium	38:71	nov., a novel endophytic actinobacterium isolated from halophytes.
30362936	1	12	theme	endophytic	46:55	arg1	nov.					32:35	nov.	32:35	nov.	32:35	nov., a novel endophytic actinobacterium isolated from halophytes.
30362936	7	13	theme	strain	825:830	arg1	303T					837:840	strain YJYP 303T	825:840	strain YJYP 303T	825:840	The average nucleotide identity values of strain YJYP 303T and YZYP 518 to M. halotolerans YIM 70130T were determined to be 79.97 and 80.03 %, respectively.
30362936	17	14	theme	YJYP	1808:1811	arg1	49100T					1840:1845	=CGMCC 1.16264T=KCTC 49100T	1819:1845	=CGMCC 1.16264T=KCTC 49100T	1819:1845	The type strain is YJYP 303T (=CGMCC 1.16264T=KCTC 49100T).
30362936	17	14	theme	YJYP	1808:1811	arg1	strain					1798:1803	The type strain	1789:1803	The type strain	1789:1803	The type strain is YJYP 303T (=CGMCC 1.16264T=KCTC 49100T).
30362936	17	14	theme	YJYP	1808:1811	arg1	303T					1813:1816	YJYP 303T	1808:1816	YJYP 303T (=CGMCC 1.16264T=KCTC 49100T)	1808:1846	The type strain is YJYP 303T (=CGMCC 1.16264T=KCTC 49100T).
30362936	12	15	theme	whole-cell	1254:1263	arg1	sugars					1265:1270	The whole-cell sugars	1250:1270	The whole-cell sugars	1250:1270	The whole-cell sugars were mannose, ribose, rhamnose, glucose, galactose and two unidentified sugars.
30362936	12	15	theme	whole-cell	1254:1263	arg1	mannose					1277:1283	mannose	1277:1283	mannose	1277:1283	The whole-cell sugars were mannose, ribose, rhamnose, glucose, galactose and two unidentified sugars.
30362936	6	16	theme	sequence	715:722	arg1	70130T					685:690	Microbacterium halotolerans YIM 70130T	653:690	Microbacterium halotolerans YIM 70130T (97.58 % 16S rRNA gene sequence identity)	653:732	In the phylogenetic tree, the two strains shared a clade with Microbacterium halotolerans YIM 70130T (97.58 % 16S rRNA gene sequence identity) and Microbacterium populi KCTC 29152T (96.54 %).
30362936	6	16	theme	sequence	715:722	arg1	identity					724:731	97.58 % 16S rRNA gene sequence identity	693:731	97.58 % 16S rRNA gene sequence identity	693:731	In the phylogenetic tree, the two strains shared a clade with Microbacterium halotolerans YIM 70130T (97.58 % 16S rRNA gene sequence identity) and Microbacterium populi KCTC 29152T (96.54 %).
30362936	10	17	theme	predominant	1122:1132	arg1	quinones					1146:1153	The predominant respiratory quinones	1118:1153	The predominant respiratory quinones	1118:1153	The predominant respiratory quinones was MK-11, followed by MK-10 and MK-12.
30362936	10	17	theme	predominant	1122:1132	arg1	MK-11					1159:1163	MK-11	1159:1163	MK-11	1159:1163	The predominant respiratory quinones was MK-11, followed by MK-10 and MK-12.
30362936	11	18	theme	peptidoglycan	1220:1232	arg1	type					1212:1215	The muramic acid type	1195:1215	The muramic acid type of peptidoglycan	1195:1232	The muramic acid type of peptidoglycan was N-glycolyl.
30362936	11	18	theme	peptidoglycan	1220:1232	arg1	N-glycolyl					1238:1247	N-glycolyl	1238:1247	N-glycolyl	1238:1247	The muramic acid type of peptidoglycan was N-glycolyl.
30362936	15	19	theme	Microbacteriumhalophytorum	1740:1765	arg1	sp					1767:1768	the name Microbacteriumhalophytorum sp	1731:1768	the name Microbacteriumhalophytorum sp	1731:1768	On the basis of the evidence presented in this study, strains YJYP 303T and YZYP 518 are characterized as members of a novel species in the genus Microbacterium, for which the name Microbacteriumhalophytorum sp.
30362936	6	20	theme	gene	710:713	arg1	70130T					685:690	Microbacterium halotolerans YIM 70130T	653:690	Microbacterium halotolerans YIM 70130T (97.58 % 16S rRNA gene sequence identity)	653:732	In the phylogenetic tree, the two strains shared a clade with Microbacterium halotolerans YIM 70130T (97.58 % 16S rRNA gene sequence identity) and Microbacterium populi KCTC 29152T (96.54 %).
30362936	6	20	theme	gene	710:713	arg1	identity					724:731	97.58 % 16S rRNA gene sequence identity	693:731	97.58 % 16S rRNA gene sequence identity	693:731	In the phylogenetic tree, the two strains shared a clade with Microbacterium halotolerans YIM 70130T (97.58 % 16S rRNA gene sequence identity) and Microbacterium populi KCTC 29152T (96.54 %).
30362936	15	21	theme	YZYP	1635:1638	arg1	518					1640:1642	YZYP 518	1635:1642	YZYP 518	1635:1642	On the basis of the evidence presented in this study, strains YJYP 303T and YZYP 518 are characterized as members of a novel species in the genus Microbacterium, for which the name Microbacteriumhalophytorum sp.
30362936	17	22	theme	type	1793:1796	arg1	strain					1798:1803	The type strain	1789:1803	The type strain	1789:1803	The type strain is YJYP 303T (=CGMCC 1.16264T=KCTC 49100T).
30362936	17	22	theme	type	1793:1796	arg1	303T					1813:1816	YJYP 303T	1808:1816	YJYP 303T (=CGMCC 1.16264T=KCTC 49100T)	1808:1846	The type strain is YJYP 303T (=CGMCC 1.16264T=KCTC 49100T).
30362936	14	23	theme	unidentified	1501:1512	arg1	phospholipid					1514:1525	an unidentified phospholipid	1498:1525	an unidentified phospholipid	1498:1525	The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid and an unidentified glycolipid.
30362936	2	24	theme	YZYP	142:145	arg1	strains					119:125	Two actinobacterial strains	99:125	Two actinobacterial strains	99:125	Two actinobacterial strains, YJYP 303T and YZYP 518, were isolated from two species of halophytes collected from the southern edge of the Gurbantunggut Desert.
30362936	2	24	theme	YZYP	142:145	arg1	518					147:149	YZYP 518	142:149	YZYP 518	142:149	Two actinobacterial strains, YJYP 303T and YZYP 518, were isolated from two species of halophytes collected from the southern edge of the Gurbantunggut Desert.
30362936	4	25	theme	strains	351:357	arg1	Growth					333:338	Growth	333:338	Growth of the two strains	333:357	Growth of the two strains was found to occur at 4-44 °C, pH 6.0-12.0 and in the presence of up to 15 % (w/v) NaCl.
30362936	15	26	theme	YJYP	1621:1624	arg1	303T					1626:1629	YJYP 303T	1621:1629	YJYP 303T	1621:1629	On the basis of the evidence presented in this study, strains YJYP 303T and YZYP 518 are characterized as members of a novel species in the genus Microbacterium, for which the name Microbacteriumhalophytorum sp.
30362936	0	27	theme	halophytorum	15:26	arg1	sp					28:29	Microbacterium halophytorum sp	0:29	Microbacterium halophytorum sp.	0:30	Microbacterium halophytorum sp.
30362936	11	28	theme	acid	1207:1210	arg1	type					1212:1215	The muramic acid type	1195:1215	The muramic acid type of peptidoglycan	1195:1232	The muramic acid type of peptidoglycan was N-glycolyl.
30362936	11	28	theme	acid	1207:1210	arg1	N-glycolyl					1238:1247	N-glycolyl	1238:1247	N-glycolyl	1238:1247	The muramic acid type of peptidoglycan was N-glycolyl.
30362936	8	29	theme	genomic	944:950	arg1	%					1024:1024	69.72 and 70.57 %	1008:1024	69.72 and 70.57 %	1008:1024	The genomic DNA G+C contents of strains YJYP 303T and YZYP 518 were 69.72 and 70.57 %, respectively.
30362936	8	29	theme	genomic	944:950	arg1	G+C contents					956:967	The genomic DNA G+C contents	940:967	The genomic DNA G+C contents of strains YJYP 303T and YZYP 518	940:1001	The genomic DNA G+C contents of strains YJYP 303T and YZYP 518 were 69.72 and 70.57 %, respectively.
30362936	6	30	theme	16S	701:703	arg1	70130T					685:690	Microbacterium halotolerans YIM 70130T	653:690	Microbacterium halotolerans YIM 70130T (97.58 % 16S rRNA gene sequence identity)	653:732	In the phylogenetic tree, the two strains shared a clade with Microbacterium halotolerans YIM 70130T (97.58 % 16S rRNA gene sequence identity) and Microbacterium populi KCTC 29152T (96.54 %).
30362936	6	30	theme	16S	701:703	arg1	identity					724:731	97.58 % 16S rRNA gene sequence identity	693:731	97.58 % 16S rRNA gene sequence identity	693:731	In the phylogenetic tree, the two strains shared a clade with Microbacterium halotolerans YIM 70130T (97.58 % 16S rRNA gene sequence identity) and Microbacterium populi KCTC 29152T (96.54 %).
30362936	7	31	theme	303T	837:840	arg1	%					923:923	79.97 and 80.03 %	907:923	79.97 and 80.03 %	907:923	The average nucleotide identity values of strain YJYP 303T and YZYP 518 to M. halotolerans YIM 70130T were determined to be 79.97 and 80.03 %, respectively.
30362936	7	31	theme	303T	837:840	arg1	values					815:820	The average nucleotide identity values	783:820	The average nucleotide identity values of strain YJYP 303T and YZYP 518 to M. halotolerans YIM 70130T	783:883	The average nucleotide identity values of strain YJYP 303T and YZYP 518 to M. halotolerans YIM 70130T were determined to be 79.97 and 80.03 %, respectively.
30362936	0	32	theme	Microbacterium	0:13	arg1	sp					28:29	Microbacterium halophytorum sp	0:29	Microbacterium halophytorum sp.	0:30	Microbacterium halophytorum sp.
30362936	4	33	dep	%	434:434	arg1	15 					431:433	15 	431:433	15 	431:433	Growth of the two strains was found to occur at 4-44 °C, pH 6.0-12.0 and in the presence of up to 15 % (w/v) NaCl.
30362936	4	33	dep	%	434:434	arg1	w/v					437:439	w/v	437:439	w/v	437:439	Growth of the two strains was found to occur at 4-44 °C, pH 6.0-12.0 and in the presence of up to 15 % (w/v) NaCl.
30362936	15	34	theme	name	1735:1738	arg1	sp					1767:1768	the name Microbacteriumhalophytorum sp	1731:1768	the name Microbacteriumhalophytorum sp	1731:1768	On the basis of the evidence presented in this study, strains YJYP 303T and YZYP 518 are characterized as members of a novel species in the genus Microbacterium, for which the name Microbacteriumhalophytorum sp.
30362936	6	35	theme	%	699:699	arg1	70130T					685:690	Microbacterium halotolerans YIM 70130T	653:690	Microbacterium halotolerans YIM 70130T (97.58 % 16S rRNA gene sequence identity)	653:732	In the phylogenetic tree, the two strains shared a clade with Microbacterium halotolerans YIM 70130T (97.58 % 16S rRNA gene sequence identity) and Microbacterium populi KCTC 29152T (96.54 %).
30362936	6	35	theme	%	699:699	arg1	identity					724:731	97.58 % 16S rRNA gene sequence identity	693:731	97.58 % 16S rRNA gene sequence identity	693:731	In the phylogenetic tree, the two strains shared a clade with Microbacterium halotolerans YIM 70130T (97.58 % 16S rRNA gene sequence identity) and Microbacterium populi KCTC 29152T (96.54 %).
30362936	15	36	from	members	1665:1671	arg1	Microbacterium					1705:1718	the genus Microbacterium	1695:1718	the genus Microbacterium	1695:1718	On the basis of the evidence presented in this study, strains YJYP 303T and YZYP 518 are characterized as members of a novel species in the genus Microbacterium, for which the name Microbacteriumhalophytorum sp.
30362936	4	37	dep	15 	431:433	arg1	to					428:429	to	428:429	to	428:429	Growth of the two strains was found to occur at 4-44 °C, pH 6.0-12.0 and in the presence of up to 15 % (w/v) NaCl.
30362936	15	38	from	Microbacterium	1705:1718	arg1	strains					1613:1619	strains	1613:1619	strains YJYP 303T and YZYP 518	1613:1642	On the basis of the evidence presented in this study, strains YJYP 303T and YZYP 518 are characterized as members of a novel species in the genus Microbacterium, for which the name Microbacteriumhalophytorum sp.
30362936	15	38	from	Microbacterium	1705:1718	arg1	members					1665:1671	members	1665:1671	members	1665:1671	On the basis of the evidence presented in this study, strains YJYP 303T and YZYP 518 are characterized as members of a novel species in the genus Microbacterium, for which the name Microbacteriumhalophytorum sp.
30362936	6	39	theme	97.58 	693:698	arg1	70130T					685:690	Microbacterium halotolerans YIM 70130T	653:690	Microbacterium halotolerans YIM 70130T (97.58 % 16S rRNA gene sequence identity)	653:732	In the phylogenetic tree, the two strains shared a clade with Microbacterium halotolerans YIM 70130T (97.58 % 16S rRNA gene sequence identity) and Microbacterium populi KCTC 29152T (96.54 %).
30362936	6	39	theme	97.58 	693:698	arg1	identity					724:731	97.58 % 16S rRNA gene sequence identity	693:731	97.58 % 16S rRNA gene sequence identity	693:731	In the phylogenetic tree, the two strains shared a clade with Microbacterium halotolerans YIM 70130T (97.58 % 16S rRNA gene sequence identity) and Microbacterium populi KCTC 29152T (96.54 %).
30362936	13	40	theme	amino	1366:1370	arg1	acids					1372:1376	The cell-wall amino acids	1352:1376	The cell-wall amino acids	1352:1376	The cell-wall amino acids were glutamic acid, ornithine, glycine and alanine.
30362936	13	40	theme	amino	1366:1370	arg1	acid					1392:1395	glutamic acid	1383:1395	glutamic acid	1383:1395	The cell-wall amino acids were glutamic acid, ornithine, glycine and alanine.
30362936	3	41	theme	Gram-stain-positive	270:288	arg1	rods					306:309	Gram-stain-positive, aerobic, short rods	270:309	Gram-stain-positive, aerobic, short rods	270:309	Cells were Gram-stain-positive, aerobic, short rods and without flagella.
30362936	15	42	dep	evidence	1579:1586	arg1	basis					1566:1570	basis	1566:1570	basis	1566:1570	On the basis of the evidence presented in this study, strains YJYP 303T and YZYP 518 are characterized as members of a novel species in the genus Microbacterium, for which the name Microbacteriumhalophytorum sp.
30362936	15	42	dep	evidence	1579:1586	arg1	the					1562:1564	the	1562:1564	the	1562:1564	On the basis of the evidence presented in this study, strains YJYP 303T and YZYP 518 are characterized as members of a novel species in the genus Microbacterium, for which the name Microbacteriumhalophytorum sp.
30362936	14	43	theme	polar	1434:1438	arg1	lipids					1440:1445	The polar lipids	1430:1445	The polar lipids	1430:1445	The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid and an unidentified glycolipid.
30362936	14	43	theme	polar	1434:1438	arg1	diphosphatidylglycerol					1452:1473	diphosphatidylglycerol	1452:1473	diphosphatidylglycerol	1452:1473	The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid and an unidentified glycolipid.
30362936	6	44	theme	phylogenetic	598:609	arg1	tree					611:614	the phylogenetic tree	594:614	the phylogenetic tree	594:614	In the phylogenetic tree, the two strains shared a clade with Microbacterium halotolerans YIM 70130T (97.58 % 16S rRNA gene sequence identity) and Microbacterium populi KCTC 29152T (96.54 %).
30362936	8	45	dep	strains	972:978	arg1	303T					985:988	YJYP 303T	980:988	YJYP 303T	980:988	The genomic DNA G+C contents of strains YJYP 303T and YZYP 518 were 69.72 and 70.57 %, respectively.
30362936	8	45	dep	strains	972:978	arg1	518					999:1001	YZYP 518	994:1001	YZYP 518	994:1001	The genomic DNA G+C contents of strains YJYP 303T and YZYP 518 were 69.72 and 70.57 %, respectively.
30362936	17	46	theme	1.16264T=KCTC	1826:1838	arg1	49100T					1840:1845	=CGMCC 1.16264T=KCTC 49100T	1819:1845	=CGMCC 1.16264T=KCTC 49100T	1819:1845	The type strain is YJYP 303T (=CGMCC 1.16264T=KCTC 49100T).
30362936	17	46	theme	1.16264T=KCTC	1826:1838	arg1	303T					1813:1816	YJYP 303T	1808:1816	YJYP 303T (=CGMCC 1.16264T=KCTC 49100T)	1808:1846	The type strain is YJYP 303T (=CGMCC 1.16264T=KCTC 49100T).
30362936	8	47	theme	strains	972:978	arg1	%					1024:1024	69.72 and 70.57 %	1008:1024	69.72 and 70.57 %	1008:1024	The genomic DNA G+C contents of strains YJYP 303T and YZYP 518 were 69.72 and 70.57 %, respectively.
30362936	8	47	theme	strains	972:978	arg1	G+C contents					956:967	The genomic DNA G+C contents	940:967	The genomic DNA G+C contents of strains YJYP 303T and YZYP 518	940:1001	The genomic DNA G+C contents of strains YJYP 303T and YZYP 518 were 69.72 and 70.57 %, respectively.
30362936	8	48	theme	DNA	952:954	arg1	%					1024:1024	69.72 and 70.57 %	1008:1024	69.72 and 70.57 %	1008:1024	The genomic DNA G+C contents of strains YJYP 303T and YZYP 518 were 69.72 and 70.57 %, respectively.
30362936	8	48	theme	DNA	952:954	arg1	G+C contents					956:967	The genomic DNA G+C contents	940:967	The genomic DNA G+C contents of strains YJYP 303T and YZYP 518	940:1001	The genomic DNA G+C contents of strains YJYP 303T and YZYP 518 were 69.72 and 70.57 %, respectively.
30362936	10	49	theme	respiratory	1134:1144	arg1	quinones					1146:1153	The predominant respiratory quinones	1118:1153	The predominant respiratory quinones	1118:1153	The predominant respiratory quinones was MK-11, followed by MK-10 and MK-12.
30362936	10	49	theme	respiratory	1134:1144	arg1	MK-11					1159:1163	MK-11	1159:1163	MK-11	1159:1163	The predominant respiratory quinones was MK-11, followed by MK-10 and MK-12.
30362936	15	50	theme	genus	1699:1703	arg1	Microbacterium					1705:1718	the genus Microbacterium	1695:1718	the genus Microbacterium	1695:1718	On the basis of the evidence presented in this study, strains YJYP 303T and YZYP 518 are characterized as members of a novel species in the genus Microbacterium, for which the name Microbacteriumhalophytorum sp.
30362936	2	51	theme	actinobacterial	103:117	arg1	strains					119:125	Two actinobacterial strains	99:125	Two actinobacterial strains	99:125	Two actinobacterial strains, YJYP 303T and YZYP 518, were isolated from two species of halophytes collected from the southern edge of the Gurbantunggut Desert.
30362936	2	51	theme	actinobacterial	103:117	arg1	303T					133:136	YJYP 303T	128:136	YJYP 303T	128:136	Two actinobacterial strains, YJYP 303T and YZYP 518, were isolated from two species of halophytes collected from the southern edge of the Gurbantunggut Desert.
30362936	2	51	theme	actinobacterial	103:117	arg1	518					147:149	YZYP 518	142:149	YZYP 518	142:149	Two actinobacterial strains, YJYP 303T and YZYP 518, were isolated from two species of halophytes collected from the southern edge of the Gurbantunggut Desert.
30362936	12	52	theme	unidentified	1331:1342	arg1	sugars					1344:1349	two unidentified sugars	1327:1349	two unidentified sugars	1327:1349	The whole-cell sugars were mannose, ribose, rhamnose, glucose, galactose and two unidentified sugars.
30362936	2	53	theme	southern	216:223	arg1	edge					225:228	the southern edge	212:228	the southern edge of the Gurbantunggut Desert	212:256	Two actinobacterial strains, YJYP 303T and YZYP 518, were isolated from two species of halophytes collected from the southern edge of the Gurbantunggut Desert.
30362936	2	54	attach	isolated	157:164	arg2	strains					119:125	Two actinobacterial strains	99:125	Two actinobacterial strains	99:125	Two actinobacterial strains, YJYP 303T and YZYP 518, were isolated from two species of halophytes collected from the southern edge of the Gurbantunggut Desert.
30362936	2	54	attach	isolated	157:164	arg2	518					147:149	YZYP 518	142:149	YZYP 518	142:149	Two actinobacterial strains, YJYP 303T and YZYP 518, were isolated from two species of halophytes collected from the southern edge of the Gurbantunggut Desert.
30362936	2	54	attach	isolated	157:164	arg2	303T					133:136	YJYP 303T	128:136	YJYP 303T	128:136	Two actinobacterial strains, YJYP 303T and YZYP 518, were isolated from two species of halophytes collected from the southern edge of the Gurbantunggut Desert.
30362936	2	54	attach	isolated	157:164	arg1	species					175:181	two species	171:181	two species of halophytes collected from the southern edge of the Gurbantunggut Desert	171:256	Two actinobacterial strains, YJYP 303T and YZYP 518, were isolated from two species of halophytes collected from the southern edge of the Gurbantunggut Desert.
30362936	15	55	theme	species	1684:1690	arg1	strains					1613:1619	strains	1613:1619	strains YJYP 303T and YZYP 518	1613:1642	On the basis of the evidence presented in this study, strains YJYP 303T and YZYP 518 are characterized as members of a novel species in the genus Microbacterium, for which the name Microbacteriumhalophytorum sp.
30362936	15	55	theme	species	1684:1690	arg1	members					1665:1671	members	1665:1671	members	1665:1671	On the basis of the evidence presented in this study, strains YJYP 303T and YZYP 518 are characterized as members of a novel species in the genus Microbacterium, for which the name Microbacteriumhalophytorum sp.
30362936	4	56	dep	NaCl	442:445	arg1	%					434:434	%	434:434	%	434:434	Growth of the two strains was found to occur at 4-44 °C, pH 6.0-12.0 and in the presence of up to 15 % (w/v) NaCl.
30362936	9	57	theme	major	1045:1049	arg1	anteiso-C15 					1068:1079	anteiso-C15 	1068:1079	anteiso-C15 	1068:1079	The major fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
30362936	9	57	theme	major	1045:1049	arg1	acids					1057:1061	The major fatty acids	1041:1061	The major fatty acids	1041:1061	The major fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
30362936	14	58	theme	unidentified	1534:1545	arg1	glycolipid					1547:1556	an unidentified glycolipid	1531:1556	an unidentified glycolipid	1531:1556	The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid and an unidentified glycolipid.
30362936	5	59	theme	16S	479:481	arg1	sequences					493:501	16S rRNA gene sequences	479:501	16S rRNA gene sequences	479:501	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the two strains are associated with members of the genus Microbacterium.
30362936	17	60	theme	=CGMCC	1819:1824	arg1	49100T					1840:1845	=CGMCC 1.16264T=KCTC 49100T	1819:1845	=CGMCC 1.16264T=KCTC 49100T	1819:1845	The type strain is YJYP 303T (=CGMCC 1.16264T=KCTC 49100T).
30362936	17	60	theme	=CGMCC	1819:1824	arg1	303T					1813:1816	YJYP 303T	1808:1816	YJYP 303T (=CGMCC 1.16264T=KCTC 49100T)	1808:1846	The type strain is YJYP 303T (=CGMCC 1.16264T=KCTC 49100T).
30362936	8	61	theme	YJYP	980:983	arg1	303T					985:988	YJYP 303T	980:988	YJYP 303T	980:988	The genomic DNA G+C contents of strains YJYP 303T and YZYP 518 were 69.72 and 70.57 %, respectively.
30362936	6	62	theme	KCTC	760:763	arg1	29152T					765:770	Microbacterium populi KCTC 29152T	738:770	Microbacterium populi KCTC 29152T (96.54 %)	738:780	In the phylogenetic tree, the two strains shared a clade with Microbacterium halotolerans YIM 70130T (97.58 % 16S rRNA gene sequence identity) and Microbacterium populi KCTC 29152T (96.54 %).
30362936	6	62	theme	KCTC	760:763	arg1	%					779:779	96.54 %	773:779	96.54 %	773:779	In the phylogenetic tree, the two strains shared a clade with Microbacterium halotolerans YIM 70130T (97.58 % 16S rRNA gene sequence identity) and Microbacterium populi KCTC 29152T (96.54 %).
30362936	6	63	theme	populi	753:758	arg1	29152T					765:770	Microbacterium populi KCTC 29152T	738:770	Microbacterium populi KCTC 29152T (96.54 %)	738:780	In the phylogenetic tree, the two strains shared a clade with Microbacterium halotolerans YIM 70130T (97.58 % 16S rRNA gene sequence identity) and Microbacterium populi KCTC 29152T (96.54 %).
30362936	6	63	theme	populi	753:758	arg1	%					779:779	96.54 %	773:779	96.54 %	773:779	In the phylogenetic tree, the two strains shared a clade with Microbacterium halotolerans YIM 70130T (97.58 % 16S rRNA gene sequence identity) and Microbacterium populi KCTC 29152T (96.54 %).
30362936	5	64	theme	rRNA	483:486	arg1	sequences					493:501	16S rRNA gene sequences	479:501	16S rRNA gene sequences	479:501	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the two strains are associated with members of the genus Microbacterium.
30362936	9	65	theme	fatty	1051:1055	arg1	anteiso-C15 					1068:1079	anteiso-C15 	1068:1079	anteiso-C15 	1068:1079	The major fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
30362936	9	65	theme	fatty	1051:1055	arg1	acids					1057:1061	The major fatty acids	1041:1061	The major fatty acids	1041:1061	The major fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
30362936	7	66	dep	YZYP	846:849	arg1	70130T					878:883	70130T	878:883	70130T	878:883	The average nucleotide identity values of strain YJYP 303T and YZYP 518 to M. halotolerans YIM 70130T were determined to be 79.97 and 80.03 %, respectively.
30362936	7	66	dep	YZYP	846:849	arg1	to					855:856	to	855:856	to	855:856	The average nucleotide identity values of strain YJYP 303T and YZYP 518 to M. halotolerans YIM 70130T were determined to be 79.97 and 80.03 %, respectively.
30362936	7	67	theme	YJYP	832:835	arg1	303T					837:840	strain YJYP 303T	825:840	strain YJYP 303T	825:840	The average nucleotide identity values of strain YJYP 303T and YZYP 518 to M. halotolerans YIM 70130T were determined to be 79.97 and 80.03 %, respectively.
30362936	8	68	theme	YZYP	994:997	arg1	518					999:1001	YZYP 518	994:1001	YZYP 518	994:1001	The genomic DNA G+C contents of strains YJYP 303T and YZYP 518 were 69.72 and 70.57 %, respectively.
30362936	2	69	theme	halophytes	186:195	arg1	species					175:181	two species	171:181	two species of halophytes collected from the southern edge of the Gurbantunggut Desert	171:256	Two actinobacterial strains, YJYP 303T and YZYP 518, were isolated from two species of halophytes collected from the southern edge of the Gurbantunggut Desert.
30362936	2	70	theme	Desert	251:256	arg1	edge					225:228	the southern edge	212:228	the southern edge of the Gurbantunggut Desert	212:256	Two actinobacterial strains, YJYP 303T and YZYP 518, were isolated from two species of halophytes collected from the southern edge of the Gurbantunggut Desert.
30362936	5	71	theme	gene	488:491	arg1	sequences					493:501	16S rRNA gene sequences	479:501	16S rRNA gene sequences	479:501	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the two strains are associated with members of the genus Microbacterium.
30362936	13	72	theme	glutamic	1383:1390	arg1	acids					1372:1376	The cell-wall amino acids	1352:1376	The cell-wall amino acids	1352:1376	The cell-wall amino acids were glutamic acid, ornithine, glycine and alanine.
30362936	13	72	theme	glutamic	1383:1390	arg1	acid					1392:1395	glutamic acid	1383:1395	glutamic acid	1383:1395	The cell-wall amino acids were glutamic acid, ornithine, glycine and alanine.
30362936	6	73	theme	halotolerans	668:679	arg1	identity					724:731	97.58 % 16S rRNA gene sequence identity	693:731	97.58 % 16S rRNA gene sequence identity	693:731	In the phylogenetic tree, the two strains shared a clade with Microbacterium halotolerans YIM 70130T (97.58 % 16S rRNA gene sequence identity) and Microbacterium populi KCTC 29152T (96.54 %).
30362936	6	73	theme	halotolerans	668:679	arg1	70130T					685:690	Microbacterium halotolerans YIM 70130T	653:690	Microbacterium halotolerans YIM 70130T (97.58 % 16S rRNA gene sequence identity)	653:732	In the phylogenetic tree, the two strains shared a clade with Microbacterium halotolerans YIM 70130T (97.58 % 16S rRNA gene sequence identity) and Microbacterium populi KCTC 29152T (96.54 %).
30362936	6	74	theme	Microbacterium	738:751	arg1	29152T					765:770	Microbacterium populi KCTC 29152T	738:770	Microbacterium populi KCTC 29152T (96.54 %)	738:780	In the phylogenetic tree, the two strains shared a clade with Microbacterium halotolerans YIM 70130T (97.58 % 16S rRNA gene sequence identity) and Microbacterium populi KCTC 29152T (96.54 %).
30362936	6	74	theme	Microbacterium	738:751	arg1	%					779:779	96.54 %	773:779	96.54 %	773:779	In the phylogenetic tree, the two strains shared a clade with Microbacterium halotolerans YIM 70130T (97.58 % 16S rRNA gene sequence identity) and Microbacterium populi KCTC 29152T (96.54 %).
30362936	2	75	theme	Gurbantunggut	237:249	arg1	Desert					251:256	the Gurbantunggut Desert	233:256	the Gurbantunggut Desert	233:256	Two actinobacterial strains, YJYP 303T and YZYP 518, were isolated from two species of halophytes collected from the southern edge of the Gurbantunggut Desert.
30362936	6	76	theme	Microbacterium	653:666	arg1	identity					724:731	97.58 % 16S rRNA gene sequence identity	693:731	97.58 % 16S rRNA gene sequence identity	693:731	In the phylogenetic tree, the two strains shared a clade with Microbacterium halotolerans YIM 70130T (97.58 % 16S rRNA gene sequence identity) and Microbacterium populi KCTC 29152T (96.54 %).
30362936	6	76	theme	Microbacterium	653:666	arg1	70130T					685:690	Microbacterium halotolerans YIM 70130T	653:690	Microbacterium halotolerans YIM 70130T (97.58 % 16S rRNA gene sequence identity)	653:732	In the phylogenetic tree, the two strains shared a clade with Microbacterium halotolerans YIM 70130T (97.58 % 16S rRNA gene sequence identity) and Microbacterium populi KCTC 29152T (96.54 %).
30362936	7	77	theme	nucleotide	795:804	arg1	identity					806:813	average nucleotide identity	787:813	The average nucleotide identity values of strain YJYP 303T and YZYP 518 to M. halotolerans YIM 70130T	783:883	The average nucleotide identity values of strain YJYP 303T and YZYP 518 to M. halotolerans YIM 70130T were determined to be 79.97 and 80.03 %, respectively.
30362936	5	78	theme	Phylogenetic	448:459	arg1	analysis					461:468	Phylogenetic analysis	448:468	Phylogenetic analysis based on 16S rRNA gene sequences	448:501	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the two strains are associated with members of the genus Microbacterium.
30362936	7	79	theme	identity	806:813	arg1	%					923:923	79.97 and 80.03 %	907:923	79.97 and 80.03 %	907:923	The average nucleotide identity values of strain YJYP 303T and YZYP 518 to M. halotolerans YIM 70130T were determined to be 79.97 and 80.03 %, respectively.
30362936	7	79	theme	identity	806:813	arg1	values					815:820	The average nucleotide identity values	783:820	The average nucleotide identity values of strain YJYP 303T and YZYP 518 to M. halotolerans YIM 70130T	783:883	The average nucleotide identity values of strain YJYP 303T and YZYP 518 to M. halotolerans YIM 70130T were determined to be 79.97 and 80.03 %, respectively.
30362936	15	80	theme	novel	1678:1682	arg1	species					1684:1690	a novel species	1676:1690	a novel species in the genus Microbacterium	1676:1718	On the basis of the evidence presented in this study, strains YJYP 303T and YZYP 518 are characterized as members of a novel species in the genus Microbacterium, for which the name Microbacteriumhalophytorum sp.
30362936	15	81	from	species	1684:1690	arg1	Microbacterium					1705:1718	the genus Microbacterium	1695:1718	the genus Microbacterium	1695:1718	On the basis of the evidence presented in this study, strains YJYP 303T and YZYP 518 are characterized as members of a novel species in the genus Microbacterium, for which the name Microbacteriumhalophytorum sp.
30362936	1	82	theme	novel	40:44	arg1	actinobacterium					57:71	a novel endophytic actinobacterium	38:71	a novel endophytic actinobacterium	38:71	nov., a novel endophytic actinobacterium isolated from halophytes.
30362936	1	82	theme	novel	40:44	arg1	nov.					32:35	nov.	32:35	nov.	32:35	nov., a novel endophytic actinobacterium isolated from halophytes.
30362936	15	83	dep	strains	1613:1619	arg1	303T					1626:1629	YJYP 303T	1621:1629	YJYP 303T	1621:1629	On the basis of the evidence presented in this study, strains YJYP 303T and YZYP 518 are characterized as members of a novel species in the genus Microbacterium, for which the name Microbacteriumhalophytorum sp.
30362936	15	83	dep	strains	1613:1619	arg1	518					1640:1642	YZYP 518	1635:1642	YZYP 518	1635:1642	On the basis of the evidence presented in this study, strains YJYP 303T and YZYP 518 are characterized as members of a novel species in the genus Microbacterium, for which the name Microbacteriumhalophytorum sp.
30362936	9	84	dep	anteiso-C15 	1068:1079	arg1	 0					1114:1115	 0	1114:1115	 0	1114:1115	The major fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
30362936	9	84	dep	anteiso-C15 	1068:1079	arg1	iso-C16 					1105:1112	iso-C16 	1105:1112	iso-C16 	1105:1112	The major fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
30362936	9	84	dep	anteiso-C15 	1068:1079	arg1	 0					1081:1082	 0	1081:1082	 0	1081:1082	The major fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
30362936	9	84	dep	anteiso-C15 	1068:1079	arg1	 0					1098:1099	 0	1098:1099	 0	1098:1099	The major fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
31408660	1	0	theme	CXC	283:285	arg1	hydroxyapatite					332:345	recombination BMP-2 (rhBMP-2)/recombination CXC chemokine ligand-13 (rhCXCL13)-loaded hollow hydroxyapatite	239:345	recombination BMP-2 (rhBMP-2)/recombination CXC chemokine ligand-13 (rhCXCL13)-loaded hollow hydroxyapatite (HA) microspheres/chitosan (CS) composite	239:387	AIMS The present study aimed to investigate the mechanism of bone repair mediated by recombination BMP-2 (rhBMP-2)/recombination CXC chemokine ligand-13 (rhCXCL13)-loaded hollow hydroxyapatite (HA) microspheres/chitosan (CS) composite.
31408660	1	0	theme	CXC	283:285	arg1	HA					348:349	HA	348:349	HA	348:349	AIMS The present study aimed to investigate the mechanism of bone repair mediated by recombination BMP-2 (rhBMP-2)/recombination CXC chemokine ligand-13 (rhCXCL13)-loaded hollow hydroxyapatite (HA) microspheres/chitosan (CS) composite.
31408660	9	1	from	roles	1587:1591	arg1	formation					1625:1633	bone formation	1620:1633	bone formation	1620:1633	The in vitro molecular experiments including RT-PCR and WB further demonstrated the roles of rhBMP-2 and rhCXCL13 in bone formation and bone repair.
31408660	9	1	from	roles	1587:1591	arg1	repair					1644:1649	bone repair	1639:1649	bone repair	1639:1649	The in vitro molecular experiments including RT-PCR and WB further demonstrated the roles of rhBMP-2 and rhCXCL13 in bone formation and bone repair.
31408660	10	2	dep	indicated	1677:1685	arg1	SIGNIFICANCE					1652:1663	SIGNIFICANCE	1652:1663	SIGNIFICANCE	1652:1663	SIGNIFICANCE Our results indicated that the hollow HA microspheres/CS composite could be effective as a delivery vehicle for rhBMP-2 and rhCXCL13 in bone regeneration and bone repair.
31408660	3	3	theme	release	555:561	arg1	solution					563:570	rhBMP-2 sustained release solution	537:570	rhBMP-2 sustained release solution on ALP activity	537:586	Secondly, the effect of rhBMP-2 sustained release solution on ALP activity and rhCXCL13 sustained release solution on cell migration of rat bone marrow mesenchyme stem cells was tested.
31408660	5	4	theme	Runx2	888:892	arg1	expression					874:883	the protein expression	862:883	the protein expression of Runx2	862:892	Finally, the mRNAs expression of osteogenic marker genes and the protein expression of Runx2 was tested by reverse transcription-polymerase chain reaction (RT-PCR) and western blotting (WB), respectively.
31408660	5	4	theme	Runx2	888:892	arg1	expression					820:829	the mRNAs expression	810:829	the mRNAs expression of osteogenic marker genes	810:856	Finally, the mRNAs expression of osteogenic marker genes and the protein expression of Runx2 was tested by reverse transcription-polymerase chain reaction (RT-PCR) and western blotting (WB), respectively.
31408660	1	5	theme	bone	215:218	arg1	repair					220:225	bone repair	215:225	bone repair mediated by recombination BMP-2 (rhBMP-2)/recombination CXC chemokine ligand-13 (rhCXCL13)-loaded hollow hydroxyapatite (HA) microspheres/chitosan (CS) composite	215:387	AIMS The present study aimed to investigate the mechanism of bone repair mediated by recombination BMP-2 (rhBMP-2)/recombination CXC chemokine ligand-13 (rhCXCL13)-loaded hollow hydroxyapatite (HA) microspheres/chitosan (CS) composite.
31408660	0	6	dep	chemokine	72:80	arg1	composite					143:151	Ligand-13-loaded hollow hydroxyapatite microspheres/chitosan composite	82:151	recombination BMP-2/ recombination CXC chemokine Ligand-13-loaded hollow hydroxyapatite microspheres/chitosan composite	33:151	Study of bone repair mediated by recombination BMP-2/ recombination CXC chemokine Ligand-13-loaded hollow hydroxyapatite microspheres/chitosan composite.
31408660	10	7	theme	bone	1823:1826	arg1	repair					1828:1833	bone repair	1823:1833	bone repair	1823:1833	SIGNIFICANCE Our results indicated that the hollow HA microspheres/CS composite could be effective as a delivery vehicle for rhBMP-2 and rhCXCL13 in bone regeneration and bone repair.
31408660	8	8	theme	bone	1490:1493	arg1	tissue					1495:1500	bone tissue	1490:1500	bone tissue	1490:1500	X-ray scoring of radius and flexural strength test showed that rhBMP-2 and rhCXCL13 could promote bone healing and improve the bending resistance of bone tissue.
31408660	11	9	theme	bone	1873:1876	arg1	regeneration					1878:1889	bone regeneration	1873:1889	bone regeneration	1873:1889	In this process, rhBMP-2 may promote bone regeneration by regulating bone marrow MSCs cells recruited by rhCXCL13.
31408660	5	10	theme	marker	845:850	arg1	genes					852:856	osteogenic marker genes	834:856	osteogenic marker genes	834:856	Finally, the mRNAs expression of osteogenic marker genes and the protein expression of Runx2 was tested by reverse transcription-polymerase chain reaction (RT-PCR) and western blotting (WB), respectively.
31408660	1	11	theme	recombination	239:251	arg1	hydroxyapatite					332:345	recombination BMP-2 (rhBMP-2)/recombination CXC chemokine ligand-13 (rhCXCL13)-loaded hollow hydroxyapatite	239:345	recombination BMP-2 (rhBMP-2)/recombination CXC chemokine ligand-13 (rhCXCL13)-loaded hollow hydroxyapatite (HA) microspheres/chitosan (CS) composite	239:387	AIMS The present study aimed to investigate the mechanism of bone repair mediated by recombination BMP-2 (rhBMP-2)/recombination CXC chemokine ligand-13 (rhCXCL13)-loaded hollow hydroxyapatite (HA) microspheres/chitosan (CS) composite.
31408660	1	11	theme	recombination	239:251	arg1	HA					348:349	HA	348:349	HA	348:349	AIMS The present study aimed to investigate the mechanism of bone repair mediated by recombination BMP-2 (rhBMP-2)/recombination CXC chemokine ligand-13 (rhCXCL13)-loaded hollow hydroxyapatite (HA) microspheres/chitosan (CS) composite.
31408660	8	12	theme	X-ray	1341:1345	arg1	scoring					1347:1353	X-ray scoring	1341:1353	X-ray scoring of radius and flexural strength test	1341:1390	X-ray scoring of radius and flexural strength test showed that rhBMP-2 and rhCXCL13 could promote bone healing and improve the bending resistance of bone tissue.
31408660	3	13	theme	release	611:617	arg1	solution					619:626	rhCXCL13 sustained release solution	592:626	rhCXCL13 sustained release solution	592:626	Secondly, the effect of rhBMP-2 sustained release solution on ALP activity and rhCXCL13 sustained release solution on cell migration of rat bone marrow mesenchyme stem cells was tested.
31408660	3	14	from	activity	579:586	arg1	effect					527:532	the effect	523:532	the effect of rhBMP-2 sustained release solution on ALP activity and rhCXCL13 sustained release solution on cell migration of rat bone marrow mesenchyme stem cells	523:685	Secondly, the effect of rhBMP-2 sustained release solution on ALP activity and rhCXCL13 sustained release solution on cell migration of rat bone marrow mesenchyme stem cells was tested.
31408660	3	15	theme	rat	649:651	arg1	cells					681:685	rat bone marrow mesenchyme stem cells	649:685	rat bone marrow mesenchyme stem cells	649:685	Secondly, the effect of rhBMP-2 sustained release solution on ALP activity and rhCXCL13 sustained release solution on cell migration of rat bone marrow mesenchyme stem cells was tested.
31408660	1	16	theme	present	163:169	arg1	study					171:175	The present study	159:175	The present study	159:175	AIMS The present study aimed to investigate the mechanism of bone repair mediated by recombination BMP-2 (rhBMP-2)/recombination CXC chemokine ligand-13 (rhCXCL13)-loaded hollow hydroxyapatite (HA) microspheres/chitosan (CS) composite.
31408660	0	17	theme	recombination	54:66	arg1	chemokine					72:80	recombination BMP-2/ recombination CXC chemokine	33:80	recombination BMP-2/ recombination CXC chemokine Ligand-13-loaded hollow hydroxyapatite microspheres/chitosan composite	33:151	Study of bone repair mediated by recombination BMP-2/ recombination CXC chemokine Ligand-13-loaded hollow hydroxyapatite microspheres/chitosan composite.
31408660	5	18	theme	genes	852:856	arg1	expression					874:883	the protein expression	862:883	the protein expression of Runx2	862:892	Finally, the mRNAs expression of osteogenic marker genes and the protein expression of Runx2 was tested by reverse transcription-polymerase chain reaction (RT-PCR) and western blotting (WB), respectively.
31408660	5	18	theme	genes	852:856	arg1	expression					820:829	the mRNAs expression	810:829	the mRNAs expression of osteogenic marker genes	810:856	Finally, the mRNAs expression of osteogenic marker genes and the protein expression of Runx2 was tested by reverse transcription-polymerase chain reaction (RT-PCR) and western blotting (WB), respectively.
31408660	5	19	theme	reverse	908:914	arg1	RT-PCR					957:962	RT-PCR	957:962	RT-PCR	957:962	Finally, the mRNAs expression of osteogenic marker genes and the protein expression of Runx2 was tested by reverse transcription-polymerase chain reaction (RT-PCR) and western blotting (WB), respectively.
31408660	5	19	theme	reverse	908:914	arg1	reaction					947:954	reverse transcription-polymerase chain reaction	908:954	reverse transcription-polymerase chain reaction (RT-PCR)	908:963	Finally, the mRNAs expression of osteogenic marker genes and the protein expression of Runx2 was tested by reverse transcription-polymerase chain reaction (RT-PCR) and western blotting (WB), respectively.
31408660	4	20	theme	differentiation	720:734	arg1	osteoblasts					708:718	osteoblasts differentiation test, X-ray scoring and three-point bending test	708:783	osteoblasts differentiation test, X-ray scoring and three-point bending test	708:783	Thirdly, osteoblasts differentiation test, X-ray scoring and three-point bending test were performed.
31408660	4	20	theme	differentiation	720:734	arg1	test					736:739	differentiation test	720:739	differentiation test	720:739	Thirdly, osteoblasts differentiation test, X-ray scoring and three-point bending test were performed.
31408660	5	21	theme	chain	941:945	arg1	RT-PCR					957:962	RT-PCR	957:962	RT-PCR	957:962	Finally, the mRNAs expression of osteogenic marker genes and the protein expression of Runx2 was tested by reverse transcription-polymerase chain reaction (RT-PCR) and western blotting (WB), respectively.
31408660	5	21	theme	chain	941:945	arg1	reaction					947:954	reverse transcription-polymerase chain reaction	908:954	reverse transcription-polymerase chain reaction (RT-PCR)	908:963	Finally, the mRNAs expression of osteogenic marker genes and the protein expression of Runx2 was tested by reverse transcription-polymerase chain reaction (RT-PCR) and western blotting (WB), respectively.
31408660	6	22	theme	FINDINGS	1010:1017	arg1	RhBMP-2					1019:1025	KEY FINDINGS RhBMP-2	1006:1025	KEY FINDINGS RhBMP-2	1006:1025	KEY FINDINGS RhBMP-2 could significantly promote the proliferation and differentiation, and RhCXCL13 could promote the migration of rat bone marrow MSCs.
31408660	0	23	theme	hollow	99:104	arg1	composite					143:151	Ligand-13-loaded hollow hydroxyapatite microspheres/chitosan composite	82:151	recombination BMP-2/ recombination CXC chemokine Ligand-13-loaded hollow hydroxyapatite microspheres/chitosan composite	33:151	Study of bone repair mediated by recombination BMP-2/ recombination CXC chemokine Ligand-13-loaded hollow hydroxyapatite microspheres/chitosan composite.
31408660	4	24	dep	osteoblasts	708:718	arg1	osteoblasts					708:718	osteoblasts differentiation test, X-ray scoring and three-point bending test	708:783	osteoblasts differentiation test, X-ray scoring and three-point bending test	708:783	Thirdly, osteoblasts differentiation test, X-ray scoring and three-point bending test were performed.
31408660	4	24	dep	osteoblasts	708:718	arg1	test					780:783	three-point bending test	760:783	three-point bending test	760:783	Thirdly, osteoblasts differentiation test, X-ray scoring and three-point bending test were performed.
31408660	4	24	dep	osteoblasts	708:718	arg1	test					736:739	differentiation test	720:739	differentiation test	720:739	Thirdly, osteoblasts differentiation test, X-ray scoring and three-point bending test were performed.
31408660	4	24	dep	osteoblasts	708:718	arg1	scoring					748:754	X-ray scoring	742:754	X-ray scoring	742:754	Thirdly, osteoblasts differentiation test, X-ray scoring and three-point bending test were performed.
31408660	2	25	dep	MATERIALS	390:398	arg1	investigated					499:510	investigated	499:510	was investigated	495:510	MATERIALS AND METHODS Firstly, the biological activity of rhBMP-2 and rhCXCL13 released from the complex was investigated.
31408660	10	26	theme	microspheres/CS	1706:1720	arg1	composite					1722:1730	the hollow HA microspheres/CS composite	1692:1730	the hollow HA microspheres/CS composite	1692:1730	SIGNIFICANCE Our results indicated that the hollow HA microspheres/CS composite could be effective as a delivery vehicle for rhBMP-2 and rhCXCL13 in bone regeneration and bone repair.
31408660	10	26	theme	microspheres/CS	1706:1720	arg1	effective					1741:1749	effective	1741:1749	effective	1741:1749	SIGNIFICANCE Our results indicated that the hollow HA microspheres/CS composite could be effective as a delivery vehicle for rhBMP-2 and rhCXCL13 in bone regeneration and bone repair.
31408660	0	27	theme	microspheres/chitosan	121:141	arg1	composite					143:151	Ligand-13-loaded hollow hydroxyapatite microspheres/chitosan composite	82:151	recombination BMP-2/ recombination CXC chemokine Ligand-13-loaded hollow hydroxyapatite microspheres/chitosan composite	33:151	Study of bone repair mediated by recombination BMP-2/ recombination CXC chemokine Ligand-13-loaded hollow hydroxyapatite microspheres/chitosan composite.
31408660	9	28	theme	rhCXCL13	1608:1615	arg1	roles					1587:1591	the roles	1583:1591	the roles of rhBMP-2 and rhCXCL13 in bone formation and bone repair	1583:1649	The in vitro molecular experiments including RT-PCR and WB further demonstrated the roles of rhBMP-2 and rhCXCL13 in bone formation and bone repair.
31408660	4	29	theme	three-point	760:770	arg1	osteoblasts					708:718	osteoblasts differentiation test, X-ray scoring and three-point bending test	708:783	osteoblasts differentiation test, X-ray scoring and three-point bending test	708:783	Thirdly, osteoblasts differentiation test, X-ray scoring and three-point bending test were performed.
31408660	4	29	theme	three-point	760:770	arg1	test					780:783	three-point bending test	760:783	three-point bending test	760:783	Thirdly, osteoblasts differentiation test, X-ray scoring and three-point bending test were performed.
31408660	10	30	theme	hollow	1696:1701	arg1	composite					1722:1730	the hollow HA microspheres/CS composite	1692:1730	the hollow HA microspheres/CS composite	1692:1730	SIGNIFICANCE Our results indicated that the hollow HA microspheres/CS composite could be effective as a delivery vehicle for rhBMP-2 and rhCXCL13 in bone regeneration and bone repair.
31408660	10	30	theme	hollow	1696:1701	arg1	effective					1741:1749	effective	1741:1749	effective	1741:1749	SIGNIFICANCE Our results indicated that the hollow HA microspheres/CS composite could be effective as a delivery vehicle for rhBMP-2 and rhCXCL13 in bone regeneration and bone repair.
31408660	5	31	theme	osteogenic	834:843	arg1	genes					852:856	osteogenic marker genes	834:856	osteogenic marker genes	834:856	Finally, the mRNAs expression of osteogenic marker genes and the protein expression of Runx2 was tested by reverse transcription-polymerase chain reaction (RT-PCR) and western blotting (WB), respectively.
31408660	5	32	theme	western	969:975	arg1	blotting					977:984	western blotting	969:984	western blotting (WB)	969:989	Finally, the mRNAs expression of osteogenic marker genes and the protein expression of Runx2 was tested by reverse transcription-polymerase chain reaction (RT-PCR) and western blotting (WB), respectively.
31408660	5	32	theme	western	969:975	arg1	WB					987:988	WB	987:988	WB	987:988	Finally, the mRNAs expression of osteogenic marker genes and the protein expression of Runx2 was tested by reverse transcription-polymerase chain reaction (RT-PCR) and western blotting (WB), respectively.
31408660	0	33	theme	bone	9:12	arg1	repair					14:19	bone repair	9:19	bone repair	9:19	Study of bone repair mediated by recombination BMP-2/ recombination CXC chemokine Ligand-13-loaded hollow hydroxyapatite microspheres/chitosan composite.
31408660	10	34	theme	bone	1801:1804	arg1	regeneration					1806:1817	bone regeneration	1801:1817	bone regeneration	1801:1817	SIGNIFICANCE Our results indicated that the hollow HA microspheres/CS composite could be effective as a delivery vehicle for rhBMP-2 and rhCXCL13 in bone regeneration and bone repair.
31408660	3	35	theme	stem	676:679	arg1	cells					681:685	rat bone marrow mesenchyme stem cells	649:685	rat bone marrow mesenchyme stem cells	649:685	Secondly, the effect of rhBMP-2 sustained release solution on ALP activity and rhCXCL13 sustained release solution on cell migration of rat bone marrow mesenchyme stem cells was tested.
31408660	2	36	theme	biological	425:434	arg1	activity					436:443	the biological activity	421:443	the biological activity of rhBMP-2 and rhCXCL13 released from the complex	421:493	MATERIALS AND METHODS Firstly, the biological activity of rhBMP-2 and rhCXCL13 released from the complex was investigated.
31408660	7	37	theme	activity	1177:1184	arg1	Detection					1160:1168	Detection	1160:1168	Detection of ALP activity and calcium salt deposition	1160:1212	Detection of ALP activity and calcium salt deposition showed that rhBMP-2 and rhCXCL13 could significantly improve the biological activity and promote cell differentiation ability.
31408660	1	38	theme	hollow	325:330	arg1	hydroxyapatite					332:345	recombination BMP-2 (rhBMP-2)/recombination CXC chemokine ligand-13 (rhCXCL13)-loaded hollow hydroxyapatite	239:345	recombination BMP-2 (rhBMP-2)/recombination CXC chemokine ligand-13 (rhCXCL13)-loaded hollow hydroxyapatite (HA) microspheres/chitosan (CS) composite	239:387	AIMS The present study aimed to investigate the mechanism of bone repair mediated by recombination BMP-2 (rhBMP-2)/recombination CXC chemokine ligand-13 (rhCXCL13)-loaded hollow hydroxyapatite (HA) microspheres/chitosan (CS) composite.
31408660	1	38	theme	hollow	325:330	arg1	HA					348:349	HA	348:349	HA	348:349	AIMS The present study aimed to investigate the mechanism of bone repair mediated by recombination BMP-2 (rhBMP-2)/recombination CXC chemokine ligand-13 (rhCXCL13)-loaded hollow hydroxyapatite (HA) microspheres/chitosan (CS) composite.
31408660	3	39	theme	marrow	658:663	arg1	cells					681:685	rat bone marrow mesenchyme stem cells	649:685	rat bone marrow mesenchyme stem cells	649:685	Secondly, the effect of rhBMP-2 sustained release solution on ALP activity and rhCXCL13 sustained release solution on cell migration of rat bone marrow mesenchyme stem cells was tested.
31408660	11	40	theme	bone	1905:1908	arg1	cells					1922:1926	bone marrow MSCs cells	1905:1926	bone marrow MSCs cells recruited by rhCXCL13	1905:1948	In this process, rhBMP-2 may promote bone regeneration by regulating bone marrow MSCs cells recruited by rhCXCL13.
31408660	0	41	theme	recombination	33:45	arg1	chemokine					72:80	recombination BMP-2/ recombination CXC chemokine	33:80	recombination BMP-2/ recombination CXC chemokine Ligand-13-loaded hollow hydroxyapatite microspheres/chitosan composite	33:151	Study of bone repair mediated by recombination BMP-2/ recombination CXC chemokine Ligand-13-loaded hollow hydroxyapatite microspheres/chitosan composite.
31408660	6	42	theme	MSCs	1154:1157	arg1	migration					1125:1133	the migration	1121:1133	the migration of rat bone marrow MSCs	1121:1157	KEY FINDINGS RhBMP-2 could significantly promote the proliferation and differentiation, and RhCXCL13 could promote the migration of rat bone marrow MSCs.
31408660	8	43	theme	bending	1468:1474	arg1	resistance					1476:1485	the bending resistance	1464:1485	the bending resistance of bone tissue	1464:1500	X-ray scoring of radius and flexural strength test showed that rhBMP-2 and rhCXCL13 could promote bone healing and improve the bending resistance of bone tissue.
31408660	1	44	theme	BMP-2	253:257	arg1	hydroxyapatite					332:345	recombination BMP-2 (rhBMP-2)/recombination CXC chemokine ligand-13 (rhCXCL13)-loaded hollow hydroxyapatite	239:345	recombination BMP-2 (rhBMP-2)/recombination CXC chemokine ligand-13 (rhCXCL13)-loaded hollow hydroxyapatite (HA) microspheres/chitosan (CS) composite	239:387	AIMS The present study aimed to investigate the mechanism of bone repair mediated by recombination BMP-2 (rhBMP-2)/recombination CXC chemokine ligand-13 (rhCXCL13)-loaded hollow hydroxyapatite (HA) microspheres/chitosan (CS) composite.
31408660	1	44	theme	BMP-2	253:257	arg1	HA					348:349	HA	348:349	HA	348:349	AIMS The present study aimed to investigate the mechanism of bone repair mediated by recombination BMP-2 (rhBMP-2)/recombination CXC chemokine ligand-13 (rhCXCL13)-loaded hollow hydroxyapatite (HA) microspheres/chitosan (CS) composite.
31408660	7	45	theme	cell	1311:1314	arg1	ability					1332:1338	cell differentiation ability	1311:1338	cell differentiation ability	1311:1338	Detection of ALP activity and calcium salt deposition showed that rhBMP-2 and rhCXCL13 could significantly improve the biological activity and promote cell differentiation ability.
31408660	9	46	dep	in	1507:1508	arg1	vitro					1510:1514	vitro	1510:1514	vitro	1510:1514	The in vitro molecular experiments including RT-PCR and WB further demonstrated the roles of rhBMP-2 and rhCXCL13 in bone formation and bone repair.
31408660	1	47	theme	rhBMP-2	260:266	arg1	hydroxyapatite					332:345	recombination BMP-2 (rhBMP-2)/recombination CXC chemokine ligand-13 (rhCXCL13)-loaded hollow hydroxyapatite	239:345	recombination BMP-2 (rhBMP-2)/recombination CXC chemokine ligand-13 (rhCXCL13)-loaded hollow hydroxyapatite (HA) microspheres/chitosan (CS) composite	239:387	AIMS The present study aimed to investigate the mechanism of bone repair mediated by recombination BMP-2 (rhBMP-2)/recombination CXC chemokine ligand-13 (rhCXCL13)-loaded hollow hydroxyapatite (HA) microspheres/chitosan (CS) composite.
31408660	1	47	theme	rhBMP-2	260:266	arg1	HA					348:349	HA	348:349	HA	348:349	AIMS The present study aimed to investigate the mechanism of bone repair mediated by recombination BMP-2 (rhBMP-2)/recombination CXC chemokine ligand-13 (rhCXCL13)-loaded hollow hydroxyapatite (HA) microspheres/chitosan (CS) composite.
31408660	5	48	theme	mRNAs	814:818	arg1	expression					820:829	the mRNAs expression	810:829	the mRNAs expression of osteogenic marker genes	810:856	Finally, the mRNAs expression of osteogenic marker genes and the protein expression of Runx2 was tested by reverse transcription-polymerase chain reaction (RT-PCR) and western blotting (WB), respectively.
31408660	11	49	theme	MSCs	1917:1920	arg1	cells					1922:1926	bone marrow MSCs cells	1905:1926	bone marrow MSCs cells recruited by rhCXCL13	1905:1948	In this process, rhBMP-2 may promote bone regeneration by regulating bone marrow MSCs cells recruited by rhCXCL13.
31408660	1	50	theme	/recombination	268:281	arg1	hydroxyapatite					332:345	recombination BMP-2 (rhBMP-2)/recombination CXC chemokine ligand-13 (rhCXCL13)-loaded hollow hydroxyapatite	239:345	recombination BMP-2 (rhBMP-2)/recombination CXC chemokine ligand-13 (rhCXCL13)-loaded hollow hydroxyapatite (HA) microspheres/chitosan (CS) composite	239:387	AIMS The present study aimed to investigate the mechanism of bone repair mediated by recombination BMP-2 (rhBMP-2)/recombination CXC chemokine ligand-13 (rhCXCL13)-loaded hollow hydroxyapatite (HA) microspheres/chitosan (CS) composite.
31408660	1	50	theme	/recombination	268:281	arg1	HA					348:349	HA	348:349	HA	348:349	AIMS The present study aimed to investigate the mechanism of bone repair mediated by recombination BMP-2 (rhBMP-2)/recombination CXC chemokine ligand-13 (rhCXCL13)-loaded hollow hydroxyapatite (HA) microspheres/chitosan (CS) composite.
31408660	7	51	theme	biological	1279:1288	arg1	activity					1290:1297	the biological activity	1275:1297	the biological activity	1275:1297	Detection of ALP activity and calcium salt deposition showed that rhBMP-2 and rhCXCL13 could significantly improve the biological activity and promote cell differentiation ability.
31408660	3	52	from	effect	527:532	arg1	activity					579:586	ALP activity	575:586	ALP activity	575:586	Secondly, the effect of rhBMP-2 sustained release solution on ALP activity and rhCXCL13 sustained release solution on cell migration of rat bone marrow mesenchyme stem cells was tested.
31408660	3	52	from	effect	527:532	arg1	migration					636:644	cell migration	631:644	cell migration of rat bone marrow mesenchyme stem cells	631:685	Secondly, the effect of rhBMP-2 sustained release solution on ALP activity and rhCXCL13 sustained release solution on cell migration of rat bone marrow mesenchyme stem cells was tested.
31408660	3	53	theme	solution	563:570	arg1	effect					527:532	the effect	523:532	the effect of rhBMP-2 sustained release solution on ALP activity and rhCXCL13 sustained release solution on cell migration of rat bone marrow mesenchyme stem cells	523:685	Secondly, the effect of rhBMP-2 sustained release solution on ALP activity and rhCXCL13 sustained release solution on cell migration of rat bone marrow mesenchyme stem cells was tested.
31408660	1	54	theme	chemokine	287:295	arg1	hydroxyapatite					332:345	recombination BMP-2 (rhBMP-2)/recombination CXC chemokine ligand-13 (rhCXCL13)-loaded hollow hydroxyapatite	239:345	recombination BMP-2 (rhBMP-2)/recombination CXC chemokine ligand-13 (rhCXCL13)-loaded hollow hydroxyapatite (HA) microspheres/chitosan (CS) composite	239:387	AIMS The present study aimed to investigate the mechanism of bone repair mediated by recombination BMP-2 (rhBMP-2)/recombination CXC chemokine ligand-13 (rhCXCL13)-loaded hollow hydroxyapatite (HA) microspheres/chitosan (CS) composite.
31408660	1	54	theme	chemokine	287:295	arg1	HA					348:349	HA	348:349	HA	348:349	AIMS The present study aimed to investigate the mechanism of bone repair mediated by recombination BMP-2 (rhBMP-2)/recombination CXC chemokine ligand-13 (rhCXCL13)-loaded hollow hydroxyapatite (HA) microspheres/chitosan (CS) composite.
31408660	8	55	theme	strength	1378:1385	arg1	test					1387:1390	flexural strength test	1369:1390	flexural strength test	1369:1390	X-ray scoring of radius and flexural strength test showed that rhBMP-2 and rhCXCL13 could promote bone healing and improve the bending resistance of bone tissue.
31408660	3	56	theme	sustained	545:553	arg1	solution					563:570	rhBMP-2 sustained release solution	537:570	rhBMP-2 sustained release solution on ALP activity	537:586	Secondly, the effect of rhBMP-2 sustained release solution on ALP activity and rhCXCL13 sustained release solution on cell migration of rat bone marrow mesenchyme stem cells was tested.
31408660	10	57	from	rhBMP-2	1777:1783	arg1	regeneration					1806:1817	bone regeneration	1801:1817	bone regeneration	1801:1817	SIGNIFICANCE Our results indicated that the hollow HA microspheres/CS composite could be effective as a delivery vehicle for rhBMP-2 and rhCXCL13 in bone regeneration and bone repair.
31408660	10	57	from	rhBMP-2	1777:1783	arg1	repair					1828:1833	bone repair	1823:1833	bone repair	1823:1833	SIGNIFICANCE Our results indicated that the hollow HA microspheres/CS composite could be effective as a delivery vehicle for rhBMP-2 and rhCXCL13 in bone regeneration and bone repair.
31408660	10	58	from	rhCXCL13	1789:1796	arg1	regeneration					1806:1817	bone regeneration	1801:1817	bone regeneration	1801:1817	SIGNIFICANCE Our results indicated that the hollow HA microspheres/CS composite could be effective as a delivery vehicle for rhBMP-2 and rhCXCL13 in bone regeneration and bone repair.
31408660	10	58	from	rhCXCL13	1789:1796	arg1	repair					1828:1833	bone repair	1823:1833	bone repair	1823:1833	SIGNIFICANCE Our results indicated that the hollow HA microspheres/CS composite could be effective as a delivery vehicle for rhBMP-2 and rhCXCL13 in bone regeneration and bone repair.
31408660	3	59	theme	ALP	575:577	arg1	activity					579:586	ALP activity	575:586	ALP activity	575:586	Secondly, the effect of rhBMP-2 sustained release solution on ALP activity and rhCXCL13 sustained release solution on cell migration of rat bone marrow mesenchyme stem cells was tested.
31408660	1	60	theme	repair	220:225	arg1	mechanism					202:210	the mechanism	198:210	the mechanism of bone repair mediated by recombination BMP-2 (rhBMP-2)/recombination CXC chemokine ligand-13 (rhCXCL13)-loaded hollow hydroxyapatite (HA) microspheres/chitosan (CS) composite	198:387	AIMS The present study aimed to investigate the mechanism of bone repair mediated by recombination BMP-2 (rhBMP-2)/recombination CXC chemokine ligand-13 (rhCXCL13)-loaded hollow hydroxyapatite (HA) microspheres/chitosan (CS) composite.
31408660	8	61	theme	tissue	1495:1500	arg1	resistance					1476:1485	the bending resistance	1464:1485	the bending resistance of bone tissue	1464:1500	X-ray scoring of radius and flexural strength test showed that rhBMP-2 and rhCXCL13 could promote bone healing and improve the bending resistance of bone tissue.
31408660	9	62	theme	bone	1620:1623	arg1	formation					1625:1633	bone formation	1620:1633	bone formation	1620:1633	The in vitro molecular experiments including RT-PCR and WB further demonstrated the roles of rhBMP-2 and rhCXCL13 in bone formation and bone repair.
31408660	11	63	theme	marrow	1910:1915	arg1	cells					1922:1926	bone marrow MSCs cells	1905:1926	bone marrow MSCs cells recruited by rhCXCL13	1905:1948	In this process, rhBMP-2 may promote bone regeneration by regulating bone marrow MSCs cells recruited by rhCXCL13.
31408660	3	64	theme	solution	619:626	arg1	effect					527:532	the effect	523:532	the effect of rhBMP-2 sustained release solution on ALP activity and rhCXCL13 sustained release solution on cell migration of rat bone marrow mesenchyme stem cells	523:685	Secondly, the effect of rhBMP-2 sustained release solution on ALP activity and rhCXCL13 sustained release solution on cell migration of rat bone marrow mesenchyme stem cells was tested.
31408660	5	65	theme	protein	866:872	arg1	expression					874:883	the protein expression	862:883	the protein expression of Runx2	862:892	Finally, the mRNAs expression of osteogenic marker genes and the protein expression of Runx2 was tested by reverse transcription-polymerase chain reaction (RT-PCR) and western blotting (WB), respectively.
31408660	3	66	theme	sustained	601:609	arg1	solution					619:626	rhCXCL13 sustained release solution	592:626	rhCXCL13 sustained release solution	592:626	Secondly, the effect of rhBMP-2 sustained release solution on ALP activity and rhCXCL13 sustained release solution on cell migration of rat bone marrow mesenchyme stem cells was tested.
31408660	4	67	theme	X-ray	742:746	arg1	osteoblasts					708:718	osteoblasts differentiation test, X-ray scoring and three-point bending test	708:783	osteoblasts differentiation test, X-ray scoring and three-point bending test	708:783	Thirdly, osteoblasts differentiation test, X-ray scoring and three-point bending test were performed.
31408660	4	67	theme	X-ray	742:746	arg1	scoring					748:754	X-ray scoring	742:754	X-ray scoring	742:754	Thirdly, osteoblasts differentiation test, X-ray scoring and three-point bending test were performed.
31408660	0	68	theme	CXC	68:70	arg1	chemokine					72:80	recombination BMP-2/ recombination CXC chemokine	33:80	recombination BMP-2/ recombination CXC chemokine Ligand-13-loaded hollow hydroxyapatite microspheres/chitosan composite	33:151	Study of bone repair mediated by recombination BMP-2/ recombination CXC chemokine Ligand-13-loaded hollow hydroxyapatite microspheres/chitosan composite.
31408660	3	69	theme	cell	631:634	arg1	migration					636:644	cell migration	631:644	cell migration of rat bone marrow mesenchyme stem cells	631:685	Secondly, the effect of rhBMP-2 sustained release solution on ALP activity and rhCXCL13 sustained release solution on cell migration of rat bone marrow mesenchyme stem cells was tested.
31408660	9	70	theme	in	1507:1508	arg1	experiments					1526:1536	The in vitro molecular experiments	1503:1536	The in vitro molecular experiments including RT-PCR and WB	1503:1560	The in vitro molecular experiments including RT-PCR and WB further demonstrated the roles of rhBMP-2 and rhCXCL13 in bone formation and bone repair.
31408660	8	71	theme	radius	1358:1363	arg1	scoring					1347:1353	X-ray scoring	1341:1353	X-ray scoring of radius and flexural strength test	1341:1390	X-ray scoring of radius and flexural strength test showed that rhBMP-2 and rhCXCL13 could promote bone healing and improve the bending resistance of bone tissue.
31408660	0	72	theme	Ligand-13-loaded	82:97	arg1	composite					143:151	Ligand-13-loaded hollow hydroxyapatite microspheres/chitosan composite	82:151	recombination BMP-2/ recombination CXC chemokine Ligand-13-loaded hollow hydroxyapatite microspheres/chitosan composite	33:151	Study of bone repair mediated by recombination BMP-2/ recombination CXC chemokine Ligand-13-loaded hollow hydroxyapatite microspheres/chitosan composite.
31408660	5	73	theme	transcription-polymerase	916:939	arg1	RT-PCR					957:962	RT-PCR	957:962	RT-PCR	957:962	Finally, the mRNAs expression of osteogenic marker genes and the protein expression of Runx2 was tested by reverse transcription-polymerase chain reaction (RT-PCR) and western blotting (WB), respectively.
31408660	5	73	theme	transcription-polymerase	916:939	arg1	reaction					947:954	reverse transcription-polymerase chain reaction	908:954	reverse transcription-polymerase chain reaction (RT-PCR)	908:963	Finally, the mRNAs expression of osteogenic marker genes and the protein expression of Runx2 was tested by reverse transcription-polymerase chain reaction (RT-PCR) and western blotting (WB), respectively.
31408660	8	74	theme	flexural	1369:1376	arg1	test					1387:1390	flexural strength test	1369:1390	flexural strength test	1369:1390	X-ray scoring of radius and flexural strength test showed that rhBMP-2 and rhCXCL13 could promote bone healing and improve the bending resistance of bone tissue.
31408660	3	75	from	solution	563:570	arg1	activity					579:586	ALP activity	575:586	ALP activity	575:586	Secondly, the effect of rhBMP-2 sustained release solution on ALP activity and rhCXCL13 sustained release solution on cell migration of rat bone marrow mesenchyme stem cells was tested.
31408660	0	76	theme	hydroxyapatite	106:119	arg1	composite					143:151	Ligand-13-loaded hollow hydroxyapatite microspheres/chitosan composite	82:151	recombination BMP-2/ recombination CXC chemokine Ligand-13-loaded hollow hydroxyapatite microspheres/chitosan composite	33:151	Study of bone repair mediated by recombination BMP-2/ recombination CXC chemokine Ligand-13-loaded hollow hydroxyapatite microspheres/chitosan composite.
31408660	6	77	theme	KEY	1006:1008	arg1	FINDINGS					1010:1017	KEY FINDINGS	1006:1017	KEY FINDINGS RhBMP-2	1006:1025	KEY FINDINGS RhBMP-2 could significantly promote the proliferation and differentiation, and RhCXCL13 could promote the migration of rat bone marrow MSCs.
31408660	9	78	theme	rhBMP-2	1596:1602	arg1	roles					1587:1591	the roles	1583:1591	the roles of rhBMP-2 and rhCXCL13 in bone formation and bone repair	1583:1649	The in vitro molecular experiments including RT-PCR and WB further demonstrated the roles of rhBMP-2 and rhCXCL13 in bone formation and bone repair.
31408660	8	79	theme	test	1387:1390	arg1	scoring					1347:1353	X-ray scoring	1341:1353	X-ray scoring of radius and flexural strength test	1341:1390	X-ray scoring of radius and flexural strength test showed that rhBMP-2 and rhCXCL13 could promote bone healing and improve the bending resistance of bone tissue.
31408660	6	80	theme	bone	1142:1145	arg1	MSCs					1154:1157	rat bone marrow MSCs	1138:1157	rat bone marrow MSCs	1138:1157	KEY FINDINGS RhBMP-2 could significantly promote the proliferation and differentiation, and RhCXCL13 could promote the migration of rat bone marrow MSCs.
31408660	1	81	theme	composite	379:387	arg1	hydroxyapatite					332:345	recombination BMP-2 (rhBMP-2)/recombination CXC chemokine ligand-13 (rhCXCL13)-loaded hollow hydroxyapatite	239:345	recombination BMP-2 (rhBMP-2)/recombination CXC chemokine ligand-13 (rhCXCL13)-loaded hollow hydroxyapatite (HA) microspheres/chitosan (CS) composite	239:387	AIMS The present study aimed to investigate the mechanism of bone repair mediated by recombination BMP-2 (rhBMP-2)/recombination CXC chemokine ligand-13 (rhCXCL13)-loaded hollow hydroxyapatite (HA) microspheres/chitosan (CS) composite.
31408660	1	81	theme	composite	379:387	arg1	HA					348:349	HA	348:349	HA	348:349	AIMS The present study aimed to investigate the mechanism of bone repair mediated by recombination BMP-2 (rhBMP-2)/recombination CXC chemokine ligand-13 (rhCXCL13)-loaded hollow hydroxyapatite (HA) microspheres/chitosan (CS) composite.
31408660	1	82	dep	AIMS	154:157	arg1	aimed					177:181	aimed	177:181	aimed to investigate the mechanism of bone repair mediated by recombination BMP-2 (rhBMP-2)/recombination CXC chemokine ligand-13 (rhCXCL13)-loaded hollow hydroxyapatite (HA) microspheres/chitosan (CS) composite	177:387	AIMS The present study aimed to investigate the mechanism of bone repair mediated by recombination BMP-2 (rhBMP-2)/recombination CXC chemokine ligand-13 (rhCXCL13)-loaded hollow hydroxyapatite (HA) microspheres/chitosan (CS) composite.
31408660	10	83	theme	HA	1703:1704	arg1	composite					1722:1730	the hollow HA microspheres/CS composite	1692:1730	the hollow HA microspheres/CS composite	1692:1730	SIGNIFICANCE Our results indicated that the hollow HA microspheres/CS composite could be effective as a delivery vehicle for rhBMP-2 and rhCXCL13 in bone regeneration and bone repair.
31408660	10	83	theme	HA	1703:1704	arg1	effective					1741:1749	effective	1741:1749	effective	1741:1749	SIGNIFICANCE Our results indicated that the hollow HA microspheres/CS composite could be effective as a delivery vehicle for rhBMP-2 and rhCXCL13 in bone regeneration and bone repair.
31408660	7	84	theme	salt	1198:1201	arg1	deposition					1203:1212	calcium salt deposition	1190:1212	calcium salt deposition	1190:1212	Detection of ALP activity and calcium salt deposition showed that rhBMP-2 and rhCXCL13 could significantly improve the biological activity and promote cell differentiation ability.
31408660	2	85	attach	released	469:476	arg2	rhBMP-2					448:454	rhBMP-2	448:454	rhBMP-2	448:454	MATERIALS AND METHODS Firstly, the biological activity of rhBMP-2 and rhCXCL13 released from the complex was investigated.
31408660	2	85	attach	released	469:476	arg1	complex					487:493	the complex	483:493	the complex	483:493	MATERIALS AND METHODS Firstly, the biological activity of rhBMP-2 and rhCXCL13 released from the complex was investigated.
31408660	2	86	theme	rhCXCL13	460:467	arg1	activity					436:443	the biological activity	421:443	the biological activity of rhBMP-2 and rhCXCL13 released from the complex	421:493	MATERIALS AND METHODS Firstly, the biological activity of rhBMP-2 and rhCXCL13 released from the complex was investigated.
31408660	0	87	theme	repair	14:19	arg1	Study					0:4	Study	0:4	Study of bone repair	0:19	Study of bone repair mediated by recombination BMP-2/ recombination CXC chemokine Ligand-13-loaded hollow hydroxyapatite microspheres/chitosan composite.
31408660	2	88	theme	rhBMP-2	448:454	arg1	activity					436:443	the biological activity	421:443	the biological activity of rhBMP-2 and rhCXCL13 released from the complex	421:493	MATERIALS AND METHODS Firstly, the biological activity of rhBMP-2 and rhCXCL13 released from the complex was investigated.
31408660	3	89	theme	mesenchyme	665:674	arg1	cells					681:685	rat bone marrow mesenchyme stem cells	649:685	rat bone marrow mesenchyme stem cells	649:685	Secondly, the effect of rhBMP-2 sustained release solution on ALP activity and rhCXCL13 sustained release solution on cell migration of rat bone marrow mesenchyme stem cells was tested.
31408660	4	90	theme	bending	772:778	arg1	osteoblasts					708:718	osteoblasts differentiation test, X-ray scoring and three-point bending test	708:783	osteoblasts differentiation test, X-ray scoring and three-point bending test	708:783	Thirdly, osteoblasts differentiation test, X-ray scoring and three-point bending test were performed.
31408660	4	90	theme	bending	772:778	arg1	test					780:783	three-point bending test	760:783	three-point bending test	760:783	Thirdly, osteoblasts differentiation test, X-ray scoring and three-point bending test were performed.
31408660	3	91	from	solution	619:626	arg1	activity					579:586	ALP activity	575:586	ALP activity	575:586	Secondly, the effect of rhBMP-2 sustained release solution on ALP activity and rhCXCL13 sustained release solution on cell migration of rat bone marrow mesenchyme stem cells was tested.
31408660	7	92	theme	ALP	1173:1175	arg1	activity					1177:1184	ALP activity	1173:1184	ALP activity	1173:1184	Detection of ALP activity and calcium salt deposition showed that rhBMP-2 and rhCXCL13 could significantly improve the biological activity and promote cell differentiation ability.
31408660	1	93	theme	-loaded	317:323	arg1	hydroxyapatite					332:345	recombination BMP-2 (rhBMP-2)/recombination CXC chemokine ligand-13 (rhCXCL13)-loaded hollow hydroxyapatite	239:345	recombination BMP-2 (rhBMP-2)/recombination CXC chemokine ligand-13 (rhCXCL13)-loaded hollow hydroxyapatite (HA) microspheres/chitosan (CS) composite	239:387	AIMS The present study aimed to investigate the mechanism of bone repair mediated by recombination BMP-2 (rhBMP-2)/recombination CXC chemokine ligand-13 (rhCXCL13)-loaded hollow hydroxyapatite (HA) microspheres/chitosan (CS) composite.
31408660	1	93	theme	-loaded	317:323	arg1	HA					348:349	HA	348:349	HA	348:349	AIMS The present study aimed to investigate the mechanism of bone repair mediated by recombination BMP-2 (rhBMP-2)/recombination CXC chemokine ligand-13 (rhCXCL13)-loaded hollow hydroxyapatite (HA) microspheres/chitosan (CS) composite.
31408660	9	94	theme	molecular	1516:1524	arg1	experiments					1526:1536	The in vitro molecular experiments	1503:1536	The in vitro molecular experiments including RT-PCR and WB	1503:1560	The in vitro molecular experiments including RT-PCR and WB further demonstrated the roles of rhBMP-2 and rhCXCL13 in bone formation and bone repair.
31408660	9	95	theme	bone	1639:1642	arg1	repair					1644:1649	bone repair	1639:1649	bone repair	1639:1649	The in vitro molecular experiments including RT-PCR and WB further demonstrated the roles of rhBMP-2 and rhCXCL13 in bone formation and bone repair.
31408660	3	96	theme	bone	653:656	arg1	cells					681:685	rat bone marrow mesenchyme stem cells	649:685	rat bone marrow mesenchyme stem cells	649:685	Secondly, the effect of rhBMP-2 sustained release solution on ALP activity and rhCXCL13 sustained release solution on cell migration of rat bone marrow mesenchyme stem cells was tested.
31408660	8	97	theme	bone	1439:1442	arg1	healing					1444:1450	bone healing	1439:1450	bone healing	1439:1450	X-ray scoring of radius and flexural strength test showed that rhBMP-2 and rhCXCL13 could promote bone healing and improve the bending resistance of bone tissue.
31408660	0	98	theme	BMP-2/	47:52	arg1	chemokine					72:80	recombination BMP-2/ recombination CXC chemokine	33:80	recombination BMP-2/ recombination CXC chemokine Ligand-13-loaded hollow hydroxyapatite microspheres/chitosan composite	33:151	Study of bone repair mediated by recombination BMP-2/ recombination CXC chemokine Ligand-13-loaded hollow hydroxyapatite microspheres/chitosan composite.
31408660	7	99	theme	differentiation	1316:1330	arg1	ability					1332:1338	cell differentiation ability	1311:1338	cell differentiation ability	1311:1338	Detection of ALP activity and calcium salt deposition showed that rhBMP-2 and rhCXCL13 could significantly improve the biological activity and promote cell differentiation ability.
31408660	10	100	theme	delivery	1756:1763	arg1	vehicle					1765:1771	a delivery vehicle	1754:1771	a delivery vehicle for rhBMP-2 and rhCXCL13 in bone regeneration and bone repair	1754:1833	SIGNIFICANCE Our results indicated that the hollow HA microspheres/CS composite could be effective as a delivery vehicle for rhBMP-2 and rhCXCL13 in bone regeneration and bone repair.
31408660	6	101	theme	marrow	1147:1152	arg1	MSCs					1154:1157	rat bone marrow MSCs	1138:1157	rat bone marrow MSCs	1138:1157	KEY FINDINGS RhBMP-2 could significantly promote the proliferation and differentiation, and RhCXCL13 could promote the migration of rat bone marrow MSCs.
31408660	3	102	theme	cells	681:685	arg1	migration					636:644	cell migration	631:644	cell migration of rat bone marrow mesenchyme stem cells	631:685	Secondly, the effect of rhBMP-2 sustained release solution on ALP activity and rhCXCL13 sustained release solution on cell migration of rat bone marrow mesenchyme stem cells was tested.
31408660	3	103	theme	rhBMP-2	537:543	arg1	solution					563:570	rhBMP-2 sustained release solution	537:570	rhBMP-2 sustained release solution on ALP activity	537:586	Secondly, the effect of rhBMP-2 sustained release solution on ALP activity and rhCXCL13 sustained release solution on cell migration of rat bone marrow mesenchyme stem cells was tested.
31408660	6	104	theme	rat	1138:1140	arg1	MSCs					1154:1157	rat bone marrow MSCs	1138:1157	rat bone marrow MSCs	1138:1157	KEY FINDINGS RhBMP-2 could significantly promote the proliferation and differentiation, and RhCXCL13 could promote the migration of rat bone marrow MSCs.
31408660	7	105	theme	calcium	1190:1196	arg1	deposition					1203:1212	calcium salt deposition	1190:1212	calcium salt deposition	1190:1212	Detection of ALP activity and calcium salt deposition showed that rhBMP-2 and rhCXCL13 could significantly improve the biological activity and promote cell differentiation ability.
31408660	7	106	theme	deposition	1203:1212	arg1	Detection					1160:1168	Detection	1160:1168	Detection of ALP activity and calcium salt deposition	1160:1212	Detection of ALP activity and calcium salt deposition showed that rhBMP-2 and rhCXCL13 could significantly improve the biological activity and promote cell differentiation ability.
31931381	0	0	theme	tomato	68:73	arg1	seedlings					75:83	tomato seedlings	68:83	tomato seedlings	68:83	Cell wall structure and composition is affected by light quality in tomato seedlings.
31931381	6	1	theme	outer	852:856	arg1	layer					858:862	the outer layer	848:862	the outer layer	848:862	However, longitudinal transmission electron microscopy indicates a high frequency of microfibrils close to parallels related to the elongation axis in the outer layer.
31931381	7	2	theme	X-ray	921:925	arg1	scattering					927:936	ultra-high resolution small angle X-ray scattering	887:936	ultra-high resolution small angle X-ray scattering	887:936	This was confirmed by ultra-high resolution small angle X-ray scattering.
31931381	13	3	theme	blue	1616:1619	arg1	light					1621:1625	blue light	1616:1625	blue light	1616:1625	Hypocotyl growth was stimulated by gibberellins under blue light.
31931381	12	4	theme	wall	1542:1545	arg1	circumstances					1547:1559	thin cell wall circumstances	1532:1559	thin cell wall circumstances	1532:1559	Auxins marginally stimulated growth in thin cell wall circumstances.
31931381	8	5	theme	longitudinal	1023:1034	arg1	direction					1036:1044	the longitudinal direction	1019:1044	the longitudinal direction	1019:1044	These data suggest that cellulose microfibrils would be passively reoriented in the longitudinal direction.
31931381	5	6	theme	inside	577:582	arg1	surface					590:596	the inside layer surface	573:596	the inside layer surface of cell wall	573:609	Additionally, the inside layer surface of cell wall presented random deposited microfibrillae angles under far-red light and in the dark.
31931381	7	7	theme	resolution	898:907	arg1	scattering					927:936	ultra-high resolution small angle X-ray scattering	887:936	ultra-high resolution small angle X-ray scattering	887:936	This was confirmed by ultra-high resolution small angle X-ray scattering.
31931381	9	8	theme	multinet	1226:1233	arg1	hypothesis					1242:1251	the multinet growth hypothesis	1222:1251	the multinet growth hypothesis	1222:1251	As the cell expands, the most recently deposited layers (inside) behave differentially oriented compared to older (outer) layers in the dark or under FR lights, agreeing with the multinet growth hypothesis.
31931381	8	9	theme	cellulose	963:971	arg1	microfibrils					973:984	cellulose microfibrils	963:984	cellulose microfibrils	963:984	These data suggest that cellulose microfibrils would be passively reoriented in the longitudinal direction.
31931381	4	10	theme	intermediate	501:512	arg1	values					514:519	intermediate values	501:519	intermediate values	501:519	Cell walls was thicker under blue and white lights and thinner under far-red light and in the dark, while intermediate values was observed for red or green lights.
31931381	3	11	theme	strong	303:308	arg1	correlation					323:333	A strong and negative correlation	301:333	A strong and negative correlation	301:333	A strong and negative correlation was found between cell wall thickness and hypocotyl growth.
31931381	9	12	theme	older	1155:1159	arg1	layers					1169:1174	older (outer) layers	1155:1174	older (outer) layers in the dark or under FR lights	1155:1205	As the cell expands, the most recently deposited layers (inside) behave differentially oriented compared to older (outer) layers in the dark or under FR lights, agreeing with the multinet growth hypothesis.
31931381	5	13	theme	layer	584:588	arg1	surface					590:596	the inside layer surface	573:596	the inside layer surface of cell wall	573:609	Additionally, the inside layer surface of cell wall presented random deposited microfibrillae angles under far-red light and in the dark.
31931381	7	14	theme	small	909:913	arg1	scattering					927:936	ultra-high resolution small angle X-ray scattering	887:936	ultra-high resolution small angle X-ray scattering	887:936	This was confirmed by ultra-high resolution small angle X-ray scattering.
31931381	9	15	dep	older	1155:1159	arg1	outer					1162:1166	outer	1162:1166	outer	1162:1166	As the cell expands, the most recently deposited layers (inside) behave differentially oriented compared to older (outer) layers in the dark or under FR lights, agreeing with the multinet growth hypothesis.
31931381	5	16	theme	far-red	666:672	arg1	light					674:678	far-red light	666:678	far-red light	666:678	Additionally, the inside layer surface of cell wall presented random deposited microfibrillae angles under far-red light and in the dark.
31931381	4	17	theme	blue	424:427	arg1	lights					439:444	blue and white lights	424:444	blue and white lights	424:444	Cell walls was thicker under blue and white lights and thinner under far-red light and in the dark, while intermediate values was observed for red or green lights.
31931381	10	18	theme	Ca	1259:1260	arg1	levels					1273:1278	High Ca and pectin levels	1254:1278	High Ca and pectin levels	1254:1278	High Ca and pectin levels were found in the cell wall of seedlings growing under blue and white light, also contributing to the low extensibility of the cell wall.
31931381	11	19	theme	Low	1418:1420	arg1	contents					1436:1443	Low Ca and pectin contents	1418:1443	Low Ca and pectin contents	1418:1443	Low Ca and pectin contents were found in the dark and under far-red light.
31931381	12	20	theme	cell	1537:1540	arg1	circumstances					1547:1559	thin cell wall circumstances	1532:1559	thin cell wall circumstances	1532:1559	Auxins marginally stimulated growth in thin cell wall circumstances.
31931381	2	21	theme	wall	269:272	arg1	structure					274:282	cell wall structure	264:282	cell wall structure	264:282	Tomato seedlings growing at different light qualities (white, blue, green, red, far-red) and in the dark displayed alterations in cell wall structure and composition.
31931381	6	22	from	axis	840:843	arg1	layer					858:862	the outer layer	848:862	the outer layer	848:862	However, longitudinal transmission electron microscopy indicates a high frequency of microfibrils close to parallels related to the elongation axis in the outer layer.
31931381	2	23	theme	Tomato	134:139	arg1	seedlings					141:149	Tomato seedlings	134:149	Tomato seedlings growing at different light qualities (white, blue, green, red, far-red) and in the dark	134:237	Tomato seedlings growing at different light qualities (white, blue, green, red, far-red) and in the dark displayed alterations in cell wall structure and composition.
31931381	6	24	theme	electron	732:739	arg1	microscopy					741:750	longitudinal transmission electron microscopy	706:750	longitudinal transmission electron microscopy	706:750	However, longitudinal transmission electron microscopy indicates a high frequency of microfibrils close to parallels related to the elongation axis in the outer layer.
31931381	10	25	theme	white	1344:1348	arg1	light					1350:1354	blue and white light	1335:1354	blue and white light	1335:1354	High Ca and pectin levels were found in the cell wall of seedlings growing under blue and white light, also contributing to the low extensibility of the cell wall.
31931381	12	26	theme	thin	1532:1535	arg1	circumstances					1547:1559	thin cell wall circumstances	1532:1559	thin cell wall circumstances	1532:1559	Auxins marginally stimulated growth in thin cell wall circumstances.
31931381	9	27	from	layers	1169:1174	arg1	dark					1183:1186	the dark	1179:1186	the dark	1179:1186	As the cell expands, the most recently deposited layers (inside) behave differentially oriented compared to older (outer) layers in the dark or under FR lights, agreeing with the multinet growth hypothesis.
31931381	4	28	theme	white	433:437	arg1	lights					439:444	blue and white lights	424:444	blue and white lights	424:444	Cell walls was thicker under blue and white lights and thinner under far-red light and in the dark, while intermediate values was observed for red or green lights.
31931381	10	29	theme	low	1382:1384	arg1	extensibility					1386:1398	the low extensibility	1378:1398	the low extensibility of the cell wall	1378:1415	High Ca and pectin levels were found in the cell wall of seedlings growing under blue and white light, also contributing to the low extensibility of the cell wall.
31931381	6	30	theme	transmission	719:730	arg1	microscopy					741:750	longitudinal transmission electron microscopy	706:750	longitudinal transmission electron microscopy	706:750	However, longitudinal transmission electron microscopy indicates a high frequency of microfibrils close to parallels related to the elongation axis in the outer layer.
31931381	10	31	theme	pectin	1266:1271	arg1	levels					1273:1278	High Ca and pectin levels	1254:1278	High Ca and pectin levels	1254:1278	High Ca and pectin levels were found in the cell wall of seedlings growing under blue and white light, also contributing to the low extensibility of the cell wall.
31931381	2	32	dep	red	209:211	arg1	far-red					214:220	far-red	214:220	far-red	214:220	Tomato seedlings growing at different light qualities (white, blue, green, red, far-red) and in the dark displayed alterations in cell wall structure and composition.
31931381	0	33	theme	wall	5:8	arg1	structure					10:18	Cell wall structure	0:18	Cell wall structure	0:18	Cell wall structure and composition is affected by light quality in tomato seedlings.
31931381	6	34	theme	longitudinal	706:717	arg1	microscopy					741:750	longitudinal transmission electron microscopy	706:750	longitudinal transmission electron microscopy	706:750	However, longitudinal transmission electron microscopy indicates a high frequency of microfibrils close to parallels related to the elongation axis in the outer layer.
31931381	2	35	theme	cell	264:267	arg1	structure					274:282	cell wall structure	264:282	cell wall structure	264:282	Tomato seedlings growing at different light qualities (white, blue, green, red, far-red) and in the dark displayed alterations in cell wall structure and composition.
31931381	6	36	theme	elongation	829:838	arg1	axis					840:843	the elongation axis	825:843	the elongation axis in the outer layer	825:862	However, longitudinal transmission electron microscopy indicates a high frequency of microfibrils close to parallels related to the elongation axis in the outer layer.
31931381	0	37	theme	Cell	0:3	arg1	structure					10:18	Cell wall structure	0:18	Cell wall structure	0:18	Cell wall structure and composition is affected by light quality in tomato seedlings.
31931381	9	38	theme	FR	1197:1198	arg1	lights					1200:1205	FR lights	1197:1205	FR lights	1197:1205	As the cell expands, the most recently deposited layers (inside) behave differentially oriented compared to older (outer) layers in the dark or under FR lights, agreeing with the multinet growth hypothesis.
31931381	10	39	theme	seedlings	1311:1319	arg1	wall					1303:1306	the cell wall	1294:1306	the cell wall of seedlings growing under blue and white light	1294:1354	High Ca and pectin levels were found in the cell wall of seedlings growing under blue and white light, also contributing to the low extensibility of the cell wall.
31931381	3	40	theme	hypocotyl	377:385	arg1	growth					387:392	hypocotyl growth	377:392	hypocotyl growth	377:392	A strong and negative correlation was found between cell wall thickness and hypocotyl growth.
31931381	3	41	theme	negative	314:321	arg1	correlation					323:333	A strong and negative correlation	301:333	A strong and negative correlation	301:333	A strong and negative correlation was found between cell wall thickness and hypocotyl growth.
31931381	10	42	located	found	1285:1289	arg2	levels					1273:1278	High Ca and pectin levels	1254:1278	High Ca and pectin levels	1254:1278	High Ca and pectin levels were found in the cell wall of seedlings growing under blue and white light, also contributing to the low extensibility of the cell wall.
31931381	10	42	located	found	1285:1289	arg1	wall					1303:1306	the cell wall	1294:1306	the cell wall of seedlings growing under blue and white light	1294:1354	High Ca and pectin levels were found in the cell wall of seedlings growing under blue and white light, also contributing to the low extensibility of the cell wall.
31931381	5	43	theme	random	621:626	arg1	angles					653:658	random deposited microfibrillae angles	621:658	random deposited microfibrillae angles	621:658	Additionally, the inside layer surface of cell wall presented random deposited microfibrillae angles under far-red light and in the dark.
31931381	7	44	theme	ultra-high	887:896	arg1	scattering					927:936	ultra-high resolution small angle X-ray scattering	887:936	ultra-high resolution small angle X-ray scattering	887:936	This was confirmed by ultra-high resolution small angle X-ray scattering.
31931381	9	45	theme	cell	1054:1057	arg1	expands					1059:1065	the cell expands	1050:1065	the cell expands	1050:1065	As the cell expands, the most recently deposited layers (inside) behave differentially oriented compared to older (outer) layers in the dark or under FR lights, agreeing with the multinet growth hypothesis.
31931381	5	46	theme	deposited	628:636	arg1	angles					653:658	random deposited microfibrillae angles	621:658	random deposited microfibrillae angles	621:658	Additionally, the inside layer surface of cell wall presented random deposited microfibrillae angles under far-red light and in the dark.
31931381	2	47	theme	light	172:176	arg1	qualities					178:186	different light qualities	162:186	different light qualities (white, blue, green, red, far-red)	162:221	Tomato seedlings growing at different light qualities (white, blue, green, red, far-red) and in the dark displayed alterations in cell wall structure and composition.
31931381	5	48	attach	presented	611:619	arg2	surface					590:596	the inside layer surface	573:596	the inside layer surface of cell wall	573:609	Additionally, the inside layer surface of cell wall presented random deposited microfibrillae angles under far-red light and in the dark.
31931381	5	48	attach	presented	611:619	arg1	dark					691:694	the dark	687:694	the dark	687:694	Additionally, the inside layer surface of cell wall presented random deposited microfibrillae angles under far-red light and in the dark.
31931381	2	49	dep	blue	196:199	arg1	green					202:206	green	202:206	green	202:206	Tomato seedlings growing at different light qualities (white, blue, green, red, far-red) and in the dark displayed alterations in cell wall structure and composition.
31931381	3	50	theme	wall	358:361	arg1	thickness					363:371	cell wall thickness	353:371	cell wall thickness	353:371	A strong and negative correlation was found between cell wall thickness and hypocotyl growth.
31931381	5	51	theme	microfibrillae	638:651	arg1	angles					653:658	random deposited microfibrillae angles	621:658	random deposited microfibrillae angles	621:658	Additionally, the inside layer surface of cell wall presented random deposited microfibrillae angles under far-red light and in the dark.
31931381	2	52	theme	different	162:170	arg1	qualities					178:186	different light qualities	162:186	different light qualities (white, blue, green, red, far-red)	162:221	Tomato seedlings growing at different light qualities (white, blue, green, red, far-red) and in the dark displayed alterations in cell wall structure and composition.
31931381	11	53	theme	pectin	1429:1434	arg1	contents					1436:1443	Low Ca and pectin contents	1418:1443	Low Ca and pectin contents	1418:1443	Low Ca and pectin contents were found in the dark and under far-red light.
31931381	11	54	theme	Ca	1422:1423	arg1	contents					1436:1443	Low Ca and pectin contents	1418:1443	Low Ca and pectin contents	1418:1443	Low Ca and pectin contents were found in the dark and under far-red light.
31931381	0	55	from	quality	57:63	arg1	seedlings					75:83	tomato seedlings	68:83	tomato seedlings	68:83	Cell wall structure and composition is affected by light quality in tomato seedlings.
31931381	10	56	theme	blue	1335:1338	arg1	light					1350:1354	blue and white light	1335:1354	blue and white light	1335:1354	High Ca and pectin levels were found in the cell wall of seedlings growing under blue and white light, also contributing to the low extensibility of the cell wall.
31931381	4	57	theme	far-red	464:470	arg1	light					472:476	far-red light	464:476	far-red light	464:476	Cell walls was thicker under blue and white lights and thinner under far-red light and in the dark, while intermediate values was observed for red or green lights.
31931381	11	58	located	found	1450:1454	arg1	dark					1463:1466	the dark	1459:1466	the dark	1459:1466	Low Ca and pectin contents were found in the dark and under far-red light.
31931381	11	58	located	found	1450:1454	arg2	contents					1436:1443	Low Ca and pectin contents	1418:1443	Low Ca and pectin contents	1418:1443	Low Ca and pectin contents were found in the dark and under far-red light.
31931381	6	59	theme	related	814:820	arg1	parallels					804:812	parallels	804:812	parallels related to the elongation axis in the outer layer	804:862	However, longitudinal transmission electron microscopy indicates a high frequency of microfibrils close to parallels related to the elongation axis in the outer layer.
31931381	9	60	theme	growth	1235:1240	arg1	hypothesis					1242:1251	the multinet growth hypothesis	1222:1251	the multinet growth hypothesis	1222:1251	As the cell expands, the most recently deposited layers (inside) behave differentially oriented compared to older (outer) layers in the dark or under FR lights, agreeing with the multinet growth hypothesis.
31931381	1	61	theme	many	100:103	arg1	aspects					105:111	many aspects	100:111	many aspects of cell development	100:131	Light affects many aspects of cell development.
31931381	3	62	theme	cell	353:356	arg1	thickness					363:371	cell wall thickness	353:371	cell wall thickness	353:371	A strong and negative correlation was found between cell wall thickness and hypocotyl growth.
31931381	10	63	theme	cell	1298:1301	arg1	wall					1303:1306	the cell wall	1294:1306	the cell wall of seedlings growing under blue and white light	1294:1354	High Ca and pectin levels were found in the cell wall of seedlings growing under blue and white light, also contributing to the low extensibility of the cell wall.
31931381	5	64	theme	cell	601:604	arg1	wall					606:609	cell wall	601:609	cell wall	601:609	Additionally, the inside layer surface of cell wall presented random deposited microfibrillae angles under far-red light and in the dark.
31931381	6	65	theme	microfibrils	782:793	arg1	frequency					769:777	a high frequency	762:777	a high frequency of microfibrils close to parallels related to the elongation axis in the outer layer	762:862	However, longitudinal transmission electron microscopy indicates a high frequency of microfibrils close to parallels related to the elongation axis in the outer layer.
31931381	13	66	theme	Hypocotyl	1562:1570	arg1	growth					1572:1577	Hypocotyl growth	1562:1577	Hypocotyl growth	1562:1577	Hypocotyl growth was stimulated by gibberellins under blue light.
31931381	10	67	theme	High	1254:1257	arg1	levels					1273:1278	High Ca and pectin levels	1254:1278	High Ca and pectin levels	1254:1278	High Ca and pectin levels were found in the cell wall of seedlings growing under blue and white light, also contributing to the low extensibility of the cell wall.
31931381	5	68	theme	wall	606:609	arg1	surface					590:596	the inside layer surface	573:596	the inside layer surface of cell wall	573:609	Additionally, the inside layer surface of cell wall presented random deposited microfibrillae angles under far-red light and in the dark.
31931381	4	69	theme	Cell	395:398	arg1	walls					400:404	Cell walls	395:404	Cell walls	395:404	Cell walls was thicker under blue and white lights and thinner under far-red light and in the dark, while intermediate values was observed for red or green lights.
31931381	9	70	theme	deposited	1086:1094	arg1	inside					1104:1109	inside	1104:1109	inside	1104:1109	As the cell expands, the most recently deposited layers (inside) behave differentially oriented compared to older (outer) layers in the dark or under FR lights, agreeing with the multinet growth hypothesis.
31931381	9	70	theme	deposited	1086:1094	arg1	layers					1096:1101	the most recently deposited layers	1068:1101	the most recently deposited layers (inside)	1068:1110	As the cell expands, the most recently deposited layers (inside) behave differentially oriented compared to older (outer) layers in the dark or under FR lights, agreeing with the multinet growth hypothesis.
31931381	11	71	theme	far-red	1478:1484	arg1	light					1486:1490	far-red light	1478:1490	far-red light	1478:1490	Low Ca and pectin contents were found in the dark and under far-red light.
31931381	4	72	theme	red	538:540	arg1	lights					551:556	red or green lights	538:556	red or green lights	538:556	Cell walls was thicker under blue and white lights and thinner under far-red light and in the dark, while intermediate values was observed for red or green lights.
31931381	1	73	theme	cell	116:119	arg1	development					121:131	cell development	116:131	cell development	116:131	Light affects many aspects of cell development.
31931381	9	74	dep	behave	1112:1117	arg1	oriented					1134:1141	oriented	1134:1141	oriented	1134:1141	As the cell expands, the most recently deposited layers (inside) behave differentially oriented compared to older (outer) layers in the dark or under FR lights, agreeing with the multinet growth hypothesis.
31931381	2	75	dep	qualities	178:186	arg1	white					189:193	white	189:193	white	189:193	Tomato seedlings growing at different light qualities (white, blue, green, red, far-red) and in the dark displayed alterations in cell wall structure and composition.
31931381	6	76	theme	high	764:767	arg1	frequency					769:777	a high frequency	762:777	a high frequency of microfibrils close to parallels related to the elongation axis in the outer layer	762:862	However, longitudinal transmission electron microscopy indicates a high frequency of microfibrils close to parallels related to the elongation axis in the outer layer.
31931381	10	77	theme	cell	1407:1410	arg1	wall					1412:1415	the cell wall	1403:1415	the cell wall	1403:1415	High Ca and pectin levels were found in the cell wall of seedlings growing under blue and white light, also contributing to the low extensibility of the cell wall.
31931381	1	78	theme	development	121:131	arg1	aspects					105:111	many aspects	100:111	many aspects of cell development	100:131	Light affects many aspects of cell development.
31931381	10	79	theme	wall	1412:1415	arg1	extensibility					1386:1398	the low extensibility	1378:1398	the low extensibility of the cell wall	1378:1415	High Ca and pectin levels were found in the cell wall of seedlings growing under blue and white light, also contributing to the low extensibility of the cell wall.
31931381	4	80	theme	green	545:549	arg1	lights					551:556	red or green lights	538:556	red or green lights	538:556	Cell walls was thicker under blue and white lights and thinner under far-red light and in the dark, while intermediate values was observed for red or green lights.
31931381	0	81	theme	light	51:55	arg1	quality					57:63	light quality	51:63	light quality in tomato seedlings	51:83	Cell wall structure and composition is affected by light quality in tomato seedlings.
31931381	7	82	theme	angle	915:919	arg1	scattering					927:936	ultra-high resolution small angle X-ray scattering	887:936	ultra-high resolution small angle X-ray scattering	887:936	This was confirmed by ultra-high resolution small angle X-ray scattering.
31931381	2	83	from	alterations	249:259	arg1	composition					288:298	composition	288:298	composition	288:298	Tomato seedlings growing at different light qualities (white, blue, green, red, far-red) and in the dark displayed alterations in cell wall structure and composition.
31931381	2	83	from	alterations	249:259	arg1	structure					274:282	cell wall structure	264:282	cell wall structure	264:282	Tomato seedlings growing at different light qualities (white, blue, green, red, far-red) and in the dark displayed alterations in cell wall structure and composition.
31931381	2	84	dep	white	189:193	arg1	blue					196:199	blue	196:199	blue	196:199	Tomato seedlings growing at different light qualities (white, blue, green, red, far-red) and in the dark displayed alterations in cell wall structure and composition.
31931381	2	84	dep	white	189:193	arg1	red					209:211	red	209:211	red	209:211	Tomato seedlings growing at different light qualities (white, blue, green, red, far-red) and in the dark displayed alterations in cell wall structure and composition.
29316991	12	0	theme	birth	1754:1758	arg1	weight					1760:1765	optimum birth weight	1746:1765	optimum birth weight	1746:1765	An appropriately balanced intake of dietary CHO and fat during pregnancy could support optimum birth weight.
29316991	10	1	theme	higher	1362:1367	arg1	intakes					1369:1375	higher intakes	1362:1375	higher intakes of glucose (10 g/d) and lactose (1 g/d)	1362:1415	Maternal diet in trimester 2 suggested that higher intakes of glucose (10 g/d) and lactose (1 g/d) were both associated with higher birth weight of 52 g (95 % CI 4, 100; P=0·03) and 5 g (95 % CI 2, 7; P<0·001) respectively.
29316991	11	2	theme	dietary	1566:1572	arg1	composition					1588:1598	dietary macronutrient composition	1566:1598	dietary macronutrient composition during pregnancy	1566:1615	These results show that dietary macronutrient composition during pregnancy is associated with birth weight outcomes.
29316991	7	3	theme	CHO	856:858	arg1	consumption					860:870	each additional 10 g/d CHO consumption	833:870	each additional 10 g/d CHO consumption	833:870	Multiple linear regression models adjusted for alcohol and smoking in trimester 1, showed that each additional 10 g/d CHO consumption was associated with an increase of 4 g (95 % CI 1, 7; P=0·003) in birth weight.
29316991	12	4	theme	fat	1711:1713	arg1	intake					1685:1690	An appropriately balanced intake	1659:1690	An appropriately balanced intake of dietary CHO and fat during pregnancy	1659:1730	An appropriately balanced intake of dietary CHO and fat during pregnancy could support optimum birth weight.
29316991	2	5	theme	differential	151:162	arg1	impact					164:169	the differential impact	147:169	the differential impact of maternal macronutrient consumption: carbohydrates (CHO), fats and protein on birth weight	147:262	There is lack of evidence on the differential impact of maternal macronutrient consumption: carbohydrates (CHO), fats and protein on birth weight.
29316991	7	6	theme	10	849:850	arg1	g/d					852:854	g/d	852:854	g/d	852:854	Multiple linear regression models adjusted for alcohol and smoking in trimester 1, showed that each additional 10 g/d CHO consumption was associated with an increase of 4 g (95 % CI 1, 7; P=0·003) in birth weight.
29316991	8	7	theme	contributing	1097:1108	arg1	macronutrients					1110:1123	energy contributing macronutrients	1090:1123	energy contributing macronutrients in each model	1090:1137	Conversely, an additional 10 g/d fat intake was associated with a lower birth weight of 8 g (95 % CI 0, 16; P=0·04) when we accounted for energy contributing macronutrients in each model, and maternal height, weight, parity, ethnicity, gestational age at delivery and sex of the baby.
29316991	4	8	from	part	494:497	arg1	UK					555:556	UK	555:556	UK	555:556	This analyses included 1,196 women with singleton pregnancies who were part of the CAffeine and REproductive health study in Leeds, UK between 2003 and 2006.
29316991	9	9	theme	association	1265:1275	arg1	evidence					1250:1257	no evidence	1247:1257	no evidence of an association between protein intake and birth weight	1247:1315	There was no evidence of an association between protein intake and birth weight.
29316991	9	10	theme	protein	1285:1291	arg1	intake					1293:1298	protein intake	1285:1298	protein intake	1285:1298	There was no evidence of an association between protein intake and birth weight.
29316991	12	11	theme	CHO	1703:1705	arg1	intake					1685:1690	An appropriately balanced intake	1659:1690	An appropriately balanced intake of dietary CHO and fat during pregnancy	1659:1730	An appropriately balanced intake of dietary CHO and fat during pregnancy could support optimum birth weight.
29316991	10	12	theme	g	1502:1502	arg1	weight					1456:1461	higher birth weight	1443:1461	higher birth weight of 52 g (95 % CI 4, 100; P=0·03) and 5 g (95 % CI 2, 7; P<0·001) respectively	1443:1539	Maternal diet in trimester 2 suggested that higher intakes of glucose (10 g/d) and lactose (1 g/d) were both associated with higher birth weight of 52 g (95 % CI 4, 100; P=0·03) and 5 g (95 % CI 2, 7; P<0·001) respectively.
29316991	12	13	theme	balanced	1676:1683	arg1	intake					1685:1690	An appropriately balanced intake	1659:1690	An appropriately balanced intake of dietary CHO and fat during pregnancy	1659:1730	An appropriately balanced intake of dietary CHO and fat during pregnancy could support optimum birth weight.
29316991	4	14	theme	singleton	463:471	arg1	pregnancies					473:483	singleton pregnancies	463:483	singleton pregnancies	463:483	This analyses included 1,196 women with singleton pregnancies who were part of the CAffeine and REproductive health study in Leeds, UK between 2003 and 2006.
29316991	7	15	dep	CI	917:918	arg1	P=0·003					926:932	P=0·003	926:932	P=0·003	926:932	Multiple linear regression models adjusted for alcohol and smoking in trimester 1, showed that each additional 10 g/d CHO consumption was associated with an increase of 4 g (95 % CI 1, 7; P=0·003) in birth weight.
29316991	7	15	dep	CI	917:918	arg1	7					923:923	7	923:923	7	923:923	Multiple linear regression models adjusted for alcohol and smoking in trimester 1, showed that each additional 10 g/d CHO consumption was associated with an increase of 4 g (95 % CI 1, 7; P=0·003) in birth weight.
29316991	11	16	theme	weight	1642:1647	arg1	outcomes					1649:1656	birth weight outcomes	1636:1656	birth weight outcomes	1636:1656	These results show that dietary macronutrient composition during pregnancy is associated with birth weight outcomes.
29316991	10	17	from	diet	1327:1330	arg1	trimester					1335:1343	trimester 2	1335:1345	trimester 2	1335:1345	Maternal diet in trimester 2 suggested that higher intakes of glucose (10 g/d) and lactose (1 g/d) were both associated with higher birth weight of 52 g (95 % CI 4, 100; P=0·03) and 5 g (95 % CI 2, 7; P<0·001) respectively.
29316991	3	18	theme	dietary	314:320	arg1	intakes					336:342	maternal dietary macronutrient intakes	305:342	maternal dietary macronutrient intakes	305:342	We investigated the association between maternal dietary macronutrient intakes and their sub-components such as saccharides and fatty acids and birth weight.
29316991	3	18	theme	dietary	314:320	arg1	saccharides					377:387	saccharides	377:387	saccharides	377:387	We investigated the association between maternal dietary macronutrient intakes and their sub-components such as saccharides and fatty acids and birth weight.
29316991	3	18	theme	dietary	314:320	arg1	acids					399:403	fatty acids	393:403	fatty acids	393:403	We investigated the association between maternal dietary macronutrient intakes and their sub-components such as saccharides and fatty acids and birth weight.
29316991	6	19	theme	Dietary	623:629	arg1	information					631:641	Dietary information	623:641	Dietary information	623:641	Dietary information was collected twice using a 24-h dietary recall about 8-12 weeks and 13-27 weeks of gestation.
29316991	8	20	from	weight	1161:1166	arg1	model					1133:1137	each model	1128:1137	each model	1128:1137	Conversely, an additional 10 g/d fat intake was associated with a lower birth weight of 8 g (95 % CI 0, 16; P=0·04) when we accounted for energy contributing macronutrients in each model, and maternal height, weight, parity, ethnicity, gestational age at delivery and sex of the baby.
29316991	8	21	dep	CI	1050:1051	arg1	P=0·04					1060:1065	P=0·04	1060:1065	P=0·04	1060:1065	Conversely, an additional 10 g/d fat intake was associated with a lower birth weight of 8 g (95 % CI 0, 16; P=0·04) when we accounted for energy contributing macronutrients in each model, and maternal height, weight, parity, ethnicity, gestational age at delivery and sex of the baby.
29316991	8	22	theme	gestational	1188:1198	arg1	age					1200:1202	gestational age	1188:1202	gestational age at delivery	1188:1214	Conversely, an additional 10 g/d fat intake was associated with a lower birth weight of 8 g (95 % CI 0, 16; P=0·04) when we accounted for energy contributing macronutrients in each model, and maternal height, weight, parity, ethnicity, gestational age at delivery and sex of the baby.
29316991	8	23	from	height	1153:1158	arg1	model					1133:1137	each model	1128:1137	each model	1128:1137	Conversely, an additional 10 g/d fat intake was associated with a lower birth weight of 8 g (95 % CI 0, 16; P=0·04) when we accounted for energy contributing macronutrients in each model, and maternal height, weight, parity, ethnicity, gestational age at delivery and sex of the baby.
29316991	10	24	theme	birth	1450:1454	arg1	weight					1456:1461	higher birth weight	1443:1461	higher birth weight of 52 g (95 % CI 4, 100; P=0·03) and 5 g (95 % CI 2, 7; P<0·001) respectively	1443:1539	Maternal diet in trimester 2 suggested that higher intakes of glucose (10 g/d) and lactose (1 g/d) were both associated with higher birth weight of 52 g (95 % CI 4, 100; P=0·03) and 5 g (95 % CI 2, 7; P<0·001) respectively.
29316991	8	25	from	ethnicity	1177:1185	arg1	model					1133:1137	each model	1128:1137	each model	1128:1137	Conversely, an additional 10 g/d fat intake was associated with a lower birth weight of 8 g (95 % CI 0, 16; P=0·04) when we accounted for energy contributing macronutrients in each model, and maternal height, weight, parity, ethnicity, gestational age at delivery and sex of the baby.
29316991	3	26	theme	fatty	393:397	arg1	acids					399:403	fatty acids	393:403	fatty acids	393:403	We investigated the association between maternal dietary macronutrient intakes and their sub-components such as saccharides and fatty acids and birth weight.
29316991	8	27	theme	fat	985:987	arg1	intake					989:994	an additional 10 g/d fat intake	964:994	an additional 10 g/d fat intake	964:994	Conversely, an additional 10 g/d fat intake was associated with a lower birth weight of 8 g (95 % CI 0, 16; P=0·04) when we accounted for energy contributing macronutrients in each model, and maternal height, weight, parity, ethnicity, gestational age at delivery and sex of the baby.
29316991	8	28	from	parity	1169:1174	arg1	model					1133:1137	each model	1128:1137	each model	1128:1137	Conversely, an additional 10 g/d fat intake was associated with a lower birth weight of 8 g (95 % CI 0, 16; P=0·04) when we accounted for energy contributing macronutrients in each model, and maternal height, weight, parity, ethnicity, gestational age at delivery and sex of the baby.
29316991	1	29	theme	observational	97:109	arg1	study					111:115	An observational study	94:115	An observational study	94:115	An observational study.
29316991	7	30	from	increase	895:902	arg1	weight					944:949	birth weight	938:949	birth weight	938:949	Multiple linear regression models adjusted for alcohol and smoking in trimester 1, showed that each additional 10 g/d CHO consumption was associated with an increase of 4 g (95 % CI 1, 7; P=0·003) in birth weight.
29316991	6	31	theme	dietary	676:682	arg1	recall					684:689	a 24-h dietary recall	669:689	a 24-h dietary recall about 8-12 weeks and 13-27 weeks of gestation	669:735	Dietary information was collected twice using a 24-h dietary recall about 8-12 weeks and 13-27 weeks of gestation.
29316991	10	32	theme	g	1469:1469	arg1	weight					1456:1461	higher birth weight	1443:1461	higher birth weight of 52 g (95 % CI 4, 100; P=0·03) and 5 g (95 % CI 2, 7; P<0·001) respectively	1443:1539	Maternal diet in trimester 2 suggested that higher intakes of glucose (10 g/d) and lactose (1 g/d) were both associated with higher birth weight of 52 g (95 % CI 4, 100; P=0·03) and 5 g (95 % CI 2, 7; P<0·001) respectively.
29316991	0	33	theme	macronutrient	11:23	arg1	composition					25:35	dietary macronutrient composition	3:35	dietary macronutrient composition during pregnancy associated with offspring birth weight	3:91	Is dietary macronutrient composition during pregnancy associated with offspring birth weight?
29316991	10	34	theme	Maternal	1318:1325	arg1	diet					1327:1330	Maternal diet	1318:1330	Maternal diet in trimester 2	1318:1345	Maternal diet in trimester 2 suggested that higher intakes of glucose (10 g/d) and lactose (1 g/d) were both associated with higher birth weight of 52 g (95 % CI 4, 100; P=0·03) and 5 g (95 % CI 2, 7; P<0·001) respectively.
29316991	2	35	theme	maternal	174:181	arg1	fats					231:234	fats	231:234	fats	231:234	There is lack of evidence on the differential impact of maternal macronutrient consumption: carbohydrates (CHO), fats and protein on birth weight.
29316991	2	35	theme	maternal	174:181	arg1	consumption					197:207	maternal macronutrient consumption: carbohydrates (CHO)	174:228	maternal macronutrient consumption: carbohydrates (CHO)	174:228	There is lack of evidence on the differential impact of maternal macronutrient consumption: carbohydrates (CHO), fats and protein on birth weight.
29316991	8	36	theme	baby	1231:1234	arg1	parity					1169:1174	parity	1169:1174	parity	1169:1174	Conversely, an additional 10 g/d fat intake was associated with a lower birth weight of 8 g (95 % CI 0, 16; P=0·04) when we accounted for energy contributing macronutrients in each model, and maternal height, weight, parity, ethnicity, gestational age at delivery and sex of the baby.
29316991	8	36	theme	baby	1231:1234	arg1	ethnicity					1177:1185	ethnicity	1177:1185	ethnicity	1177:1185	Conversely, an additional 10 g/d fat intake was associated with a lower birth weight of 8 g (95 % CI 0, 16; P=0·04) when we accounted for energy contributing macronutrients in each model, and maternal height, weight, parity, ethnicity, gestational age at delivery and sex of the baby.
29316991	8	36	theme	baby	1231:1234	arg1	macronutrients					1110:1123	energy contributing macronutrients	1090:1123	energy contributing macronutrients in each model	1090:1137	Conversely, an additional 10 g/d fat intake was associated with a lower birth weight of 8 g (95 % CI 0, 16; P=0·04) when we accounted for energy contributing macronutrients in each model, and maternal height, weight, parity, ethnicity, gestational age at delivery and sex of the baby.
29316991	8	36	theme	baby	1231:1234	arg1	sex					1220:1222	sex	1220:1222	sex	1220:1222	Conversely, an additional 10 g/d fat intake was associated with a lower birth weight of 8 g (95 % CI 0, 16; P=0·04) when we accounted for energy contributing macronutrients in each model, and maternal height, weight, parity, ethnicity, gestational age at delivery and sex of the baby.
29316991	8	36	theme	baby	1231:1234	arg1	height					1153:1158	maternal height	1144:1158	maternal height	1144:1158	Conversely, an additional 10 g/d fat intake was associated with a lower birth weight of 8 g (95 % CI 0, 16; P=0·04) when we accounted for energy contributing macronutrients in each model, and maternal height, weight, parity, ethnicity, gestational age at delivery and sex of the baby.
29316991	8	36	theme	baby	1231:1234	arg1	weight					1161:1166	weight	1161:1166	weight	1161:1166	Conversely, an additional 10 g/d fat intake was associated with a lower birth weight of 8 g (95 % CI 0, 16; P=0·04) when we accounted for energy contributing macronutrients in each model, and maternal height, weight, parity, ethnicity, gestational age at delivery and sex of the baby.
29316991	8	36	theme	baby	1231:1234	arg1	age					1200:1202	gestational age	1188:1202	gestational age at delivery	1188:1214	Conversely, an additional 10 g/d fat intake was associated with a lower birth weight of 8 g (95 % CI 0, 16; P=0·04) when we accounted for energy contributing macronutrients in each model, and maternal height, weight, parity, ethnicity, gestational age at delivery and sex of the baby.
29316991	4	37	theme	health	532:537	arg1	study					539:543	the CAffeine and REproductive health study	502:543	the CAffeine and REproductive health study	502:543	This analyses included 1,196 women with singleton pregnancies who were part of the CAffeine and REproductive health study in Leeds, UK between 2003 and 2006.
29316991	10	38	dep	g	1469:1469	arg1	%					1475:1475	95 % CI	1472:1478	95 % CI	1472:1478	Maternal diet in trimester 2 suggested that higher intakes of glucose (10 g/d) and lactose (1 g/d) were both associated with higher birth weight of 52 g (95 % CI 4, 100; P=0·03) and 5 g (95 % CI 2, 7; P<0·001) respectively.
29316991	8	39	theme	lower	1018:1022	arg1	weight					1030:1035	a lower birth weight	1016:1035	a lower birth weight of 8 g	1016:1042	Conversely, an additional 10 g/d fat intake was associated with a lower birth weight of 8 g (95 % CI 0, 16; P=0·04) when we accounted for energy contributing macronutrients in each model, and maternal height, weight, parity, ethnicity, gestational age at delivery and sex of the baby.
29316991	8	40	from	age	1200:1202	arg1	model					1133:1137	each model	1128:1137	each model	1128:1137	Conversely, an additional 10 g/d fat intake was associated with a lower birth weight of 8 g (95 % CI 0, 16; P=0·04) when we accounted for energy contributing macronutrients in each model, and maternal height, weight, parity, ethnicity, gestational age at delivery and sex of the baby.
29316991	7	41	theme	linear	747:752	arg1	models					765:770	Multiple linear regression models	738:770	Multiple linear regression models	738:770	Multiple linear regression models adjusted for alcohol and smoking in trimester 1, showed that each additional 10 g/d CHO consumption was associated with an increase of 4 g (95 % CI 1, 7; P=0·003) in birth weight.
29316991	2	42	dep	consumption	197:207	arg1	CHO					225:227	CHO	225:227	CHO	225:227	There is lack of evidence on the differential impact of maternal macronutrient consumption: carbohydrates (CHO), fats and protein on birth weight.
29316991	2	42	dep	consumption	197:207	arg1	carbohydrates					210:222	carbohydrates	210:222	maternal macronutrient consumption: carbohydrates (CHO)	174:228	There is lack of evidence on the differential impact of maternal macronutrient consumption: carbohydrates (CHO), fats and protein on birth weight.
29316991	10	43	theme	glucose	1380:1386	arg1	intakes					1369:1375	higher intakes	1362:1375	higher intakes of glucose (10 g/d) and lactose (1 g/d)	1362:1415	Maternal diet in trimester 2 suggested that higher intakes of glucose (10 g/d) and lactose (1 g/d) were both associated with higher birth weight of 52 g (95 % CI 4, 100; P=0·03) and 5 g (95 % CI 2, 7; P<0·001) respectively.
29316991	2	44	theme	consumption	197:207	arg1	impact					164:169	the differential impact	147:169	the differential impact of maternal macronutrient consumption: carbohydrates (CHO), fats and protein on birth weight	147:262	There is lack of evidence on the differential impact of maternal macronutrient consumption: carbohydrates (CHO), fats and protein on birth weight.
29316991	11	45	theme	macronutrient	1574:1586	arg1	composition					1588:1598	dietary macronutrient composition	1566:1598	dietary macronutrient composition during pregnancy	1566:1615	These results show that dietary macronutrient composition during pregnancy is associated with birth weight outcomes.
29316991	2	46	from	impact	164:169	arg1	weight					257:262	birth weight	251:262	birth weight	251:262	There is lack of evidence on the differential impact of maternal macronutrient consumption: carbohydrates (CHO), fats and protein on birth weight.
29316991	6	47	theme	13-27	712:716	arg1	weeks					718:722	13-27 weeks	712:722	13-27 weeks	712:722	Dietary information was collected twice using a 24-h dietary recall about 8-12 weeks and 13-27 weeks of gestation.
29316991	8	48	from	sex	1220:1222	arg1	model					1133:1137	each model	1128:1137	each model	1128:1137	Conversely, an additional 10 g/d fat intake was associated with a lower birth weight of 8 g (95 % CI 0, 16; P=0·04) when we accounted for energy contributing macronutrients in each model, and maternal height, weight, parity, ethnicity, gestational age at delivery and sex of the baby.
29316991	12	49	theme	optimum	1746:1752	arg1	weight					1760:1765	optimum birth weight	1746:1765	optimum birth weight	1746:1765	An appropriately balanced intake of dietary CHO and fat during pregnancy could support optimum birth weight.
29316991	8	50	from	delivery	1207:1214	arg1	parity					1169:1174	parity	1169:1174	parity	1169:1174	Conversely, an additional 10 g/d fat intake was associated with a lower birth weight of 8 g (95 % CI 0, 16; P=0·04) when we accounted for energy contributing macronutrients in each model, and maternal height, weight, parity, ethnicity, gestational age at delivery and sex of the baby.
29316991	8	50	from	delivery	1207:1214	arg1	ethnicity					1177:1185	ethnicity	1177:1185	ethnicity	1177:1185	Conversely, an additional 10 g/d fat intake was associated with a lower birth weight of 8 g (95 % CI 0, 16; P=0·04) when we accounted for energy contributing macronutrients in each model, and maternal height, weight, parity, ethnicity, gestational age at delivery and sex of the baby.
29316991	8	50	from	delivery	1207:1214	arg1	macronutrients					1110:1123	energy contributing macronutrients	1090:1123	energy contributing macronutrients in each model	1090:1137	Conversely, an additional 10 g/d fat intake was associated with a lower birth weight of 8 g (95 % CI 0, 16; P=0·04) when we accounted for energy contributing macronutrients in each model, and maternal height, weight, parity, ethnicity, gestational age at delivery and sex of the baby.
29316991	8	50	from	delivery	1207:1214	arg1	sex					1220:1222	sex	1220:1222	sex	1220:1222	Conversely, an additional 10 g/d fat intake was associated with a lower birth weight of 8 g (95 % CI 0, 16; P=0·04) when we accounted for energy contributing macronutrients in each model, and maternal height, weight, parity, ethnicity, gestational age at delivery and sex of the baby.
29316991	8	50	from	delivery	1207:1214	arg1	height					1153:1158	maternal height	1144:1158	maternal height	1144:1158	Conversely, an additional 10 g/d fat intake was associated with a lower birth weight of 8 g (95 % CI 0, 16; P=0·04) when we accounted for energy contributing macronutrients in each model, and maternal height, weight, parity, ethnicity, gestational age at delivery and sex of the baby.
29316991	8	50	from	delivery	1207:1214	arg1	weight					1161:1166	weight	1161:1166	weight	1161:1166	Conversely, an additional 10 g/d fat intake was associated with a lower birth weight of 8 g (95 % CI 0, 16; P=0·04) when we accounted for energy contributing macronutrients in each model, and maternal height, weight, parity, ethnicity, gestational age at delivery and sex of the baby.
29316991	8	50	from	delivery	1207:1214	arg1	age					1200:1202	gestational age	1188:1202	gestational age at delivery	1188:1214	Conversely, an additional 10 g/d fat intake was associated with a lower birth weight of 8 g (95 % CI 0, 16; P=0·04) when we accounted for energy contributing macronutrients in each model, and maternal height, weight, parity, ethnicity, gestational age at delivery and sex of the baby.
29316991	8	51	theme	%	1048:1048	arg1	CI					1050:1051	95 % CI 0	1045:1053	95 % CI 0	1045:1053	Conversely, an additional 10 g/d fat intake was associated with a lower birth weight of 8 g (95 % CI 0, 16; P=0·04) when we accounted for energy contributing macronutrients in each model, and maternal height, weight, parity, ethnicity, gestational age at delivery and sex of the baby.
29316991	7	52	theme	g/d	852:854	arg1	consumption					860:870	each additional 10 g/d CHO consumption	833:870	each additional 10 g/d CHO consumption	833:870	Multiple linear regression models adjusted for alcohol and smoking in trimester 1, showed that each additional 10 g/d CHO consumption was associated with an increase of 4 g (95 % CI 1, 7; P=0·003) in birth weight.
29316991	8	53	theme	energy	1090:1095	arg1	macronutrients					1110:1123	energy contributing macronutrients	1090:1123	energy contributing macronutrients in each model	1090:1137	Conversely, an additional 10 g/d fat intake was associated with a lower birth weight of 8 g (95 % CI 0, 16; P=0·04) when we accounted for energy contributing macronutrients in each model, and maternal height, weight, parity, ethnicity, gestational age at delivery and sex of the baby.
29316991	2	54	theme	evidence	135:142	arg1	lack					127:130	lack	127:130	lack of evidence	127:142	There is lack of evidence on the differential impact of maternal macronutrient consumption: carbohydrates (CHO), fats and protein on birth weight.
29316991	10	55	theme	%	1508:1508	arg1	CI					1510:1511	95 % CI 2	1505:1513	95 % CI 2	1505:1513	Maternal diet in trimester 2 suggested that higher intakes of glucose (10 g/d) and lactose (1 g/d) were both associated with higher birth weight of 52 g (95 % CI 4, 100; P=0·03) and 5 g (95 % CI 2, 7; P<0·001) respectively.
29316991	10	55	theme	%	1508:1508	arg1	g					1502:1502	5 g	1500:1502	5 g (95 % CI 2, 7; P<0·001) respectively	1500:1539	Maternal diet in trimester 2 suggested that higher intakes of glucose (10 g/d) and lactose (1 g/d) were both associated with higher birth weight of 52 g (95 % CI 4, 100; P=0·03) and 5 g (95 % CI 2, 7; P<0·001) respectively.
29316991	7	56	theme	additional	838:847	arg1	consumption					860:870	each additional 10 g/d CHO consumption	833:870	each additional 10 g/d CHO consumption	833:870	Multiple linear regression models adjusted for alcohol and smoking in trimester 1, showed that each additional 10 g/d CHO consumption was associated with an increase of 4 g (95 % CI 1, 7; P=0·003) in birth weight.
29316991	10	57	theme	lactose	1401:1407	arg1	intakes					1369:1375	higher intakes	1362:1375	higher intakes of glucose (10 g/d) and lactose (1 g/d)	1362:1415	Maternal diet in trimester 2 suggested that higher intakes of glucose (10 g/d) and lactose (1 g/d) were both associated with higher birth weight of 52 g (95 % CI 4, 100; P=0·03) and 5 g (95 % CI 2, 7; P<0·001) respectively.
29316991	12	58	theme	dietary	1695:1701	arg1	CHO					1703:1705	dietary CHO	1695:1705	dietary CHO	1695:1705	An appropriately balanced intake of dietary CHO and fat during pregnancy could support optimum birth weight.
29316991	10	59	dep	CI	1510:1511	arg1	P<0·001					1519:1525	P<0·001	1519:1525	P<0·001	1519:1525	Maternal diet in trimester 2 suggested that higher intakes of glucose (10 g/d) and lactose (1 g/d) were both associated with higher birth weight of 52 g (95 % CI 4, 100; P=0·03) and 5 g (95 % CI 2, 7; P<0·001) respectively.
29316991	10	59	dep	CI	1510:1511	arg1	7					1516:1516	7	1516:1516	7	1516:1516	Maternal diet in trimester 2 suggested that higher intakes of glucose (10 g/d) and lactose (1 g/d) were both associated with higher birth weight of 52 g (95 % CI 4, 100; P=0·03) and 5 g (95 % CI 2, 7; P<0·001) respectively.
29316991	9	60	theme	birth	1304:1308	arg1	weight					1310:1315	birth weight	1304:1315	birth weight	1304:1315	There was no evidence of an association between protein intake and birth weight.
29316991	8	61	dep	associated	1000:1009	arg1	CI					1050:1051	95 % CI 0	1045:1053	95 % CI 0	1045:1053	Conversely, an additional 10 g/d fat intake was associated with a lower birth weight of 8 g (95 % CI 0, 16; P=0·04) when we accounted for energy contributing macronutrients in each model, and maternal height, weight, parity, ethnicity, gestational age at delivery and sex of the baby.
29316991	8	62	from	macronutrients	1110:1123	arg1	model					1133:1137	each model	1128:1137	each model	1128:1137	Conversely, an additional 10 g/d fat intake was associated with a lower birth weight of 8 g (95 % CI 0, 16; P=0·04) when we accounted for energy contributing macronutrients in each model, and maternal height, weight, parity, ethnicity, gestational age at delivery and sex of the baby.
29316991	3	63	theme	maternal	305:312	arg1	intakes					336:342	maternal dietary macronutrient intakes	305:342	maternal dietary macronutrient intakes	305:342	We investigated the association between maternal dietary macronutrient intakes and their sub-components such as saccharides and fatty acids and birth weight.
29316991	3	63	theme	maternal	305:312	arg1	saccharides					377:387	saccharides	377:387	saccharides	377:387	We investigated the association between maternal dietary macronutrient intakes and their sub-components such as saccharides and fatty acids and birth weight.
29316991	3	63	theme	maternal	305:312	arg1	acids					399:403	fatty acids	393:403	fatty acids	393:403	We investigated the association between maternal dietary macronutrient intakes and their sub-components such as saccharides and fatty acids and birth weight.
29316991	8	64	theme	maternal	1144:1151	arg1	height					1153:1158	maternal height	1144:1158	maternal height	1144:1158	Conversely, an additional 10 g/d fat intake was associated with a lower birth weight of 8 g (95 % CI 0, 16; P=0·04) when we accounted for energy contributing macronutrients in each model, and maternal height, weight, parity, ethnicity, gestational age at delivery and sex of the baby.
29316991	2	65	theme	birth	251:255	arg1	weight					257:262	birth weight	251:262	birth weight	251:262	There is lack of evidence on the differential impact of maternal macronutrient consumption: carbohydrates (CHO), fats and protein on birth weight.
29316991	7	66	theme	birth	938:942	arg1	weight					944:949	birth weight	938:949	birth weight	938:949	Multiple linear regression models adjusted for alcohol and smoking in trimester 1, showed that each additional 10 g/d CHO consumption was associated with an increase of 4 g (95 % CI 1, 7; P=0·003) in birth weight.
29316991	0	67	theme	birth	80:84	arg1	weight					86:91	birth weight	80:91	birth weight	80:91	Is dietary macronutrient composition during pregnancy associated with offspring birth weight?
29316991	2	68	theme	protein	240:246	arg1	impact					164:169	the differential impact	147:169	the differential impact of maternal macronutrient consumption: carbohydrates (CHO), fats and protein on birth weight	147:262	There is lack of evidence on the differential impact of maternal macronutrient consumption: carbohydrates (CHO), fats and protein on birth weight.
29316991	4	69	theme	CAffeine	506:513	arg1	study					539:543	the CAffeine and REproductive health study	502:543	the CAffeine and REproductive health study	502:543	This analyses included 1,196 women with singleton pregnancies who were part of the CAffeine and REproductive health study in Leeds, UK between 2003 and 2006.
29316991	3	70	theme	macronutrient	322:334	arg1	intakes					336:342	maternal dietary macronutrient intakes	305:342	maternal dietary macronutrient intakes	305:342	We investigated the association between maternal dietary macronutrient intakes and their sub-components such as saccharides and fatty acids and birth weight.
29316991	3	70	theme	macronutrient	322:334	arg1	saccharides					377:387	saccharides	377:387	saccharides	377:387	We investigated the association between maternal dietary macronutrient intakes and their sub-components such as saccharides and fatty acids and birth weight.
29316991	3	70	theme	macronutrient	322:334	arg1	acids					399:403	fatty acids	393:403	fatty acids	393:403	We investigated the association between maternal dietary macronutrient intakes and their sub-components such as saccharides and fatty acids and birth weight.
29316991	4	71	theme	REproductive	519:530	arg1	study					539:543	the CAffeine and REproductive health study	502:543	the CAffeine and REproductive health study	502:543	This analyses included 1,196 women with singleton pregnancies who were part of the CAffeine and REproductive health study in Leeds, UK between 2003 and 2006.
29316991	8	72	theme	additional	967:976	arg1	intake					989:994	an additional 10 g/d fat intake	964:994	an additional 10 g/d fat intake	964:994	Conversely, an additional 10 g/d fat intake was associated with a lower birth weight of 8 g (95 % CI 0, 16; P=0·04) when we accounted for energy contributing macronutrients in each model, and maternal height, weight, parity, ethnicity, gestational age at delivery and sex of the baby.
29316991	7	73	theme	%	915:915	arg1	CI					917:918	95 % CI 1	912:920	95 % CI 1	912:920	Multiple linear regression models adjusted for alcohol and smoking in trimester 1, showed that each additional 10 g/d CHO consumption was associated with an increase of 4 g (95 % CI 1, 7; P=0·003) in birth weight.
29316991	7	73	theme	%	915:915	arg1	g					909:909	4 g	907:909	4 g (95 % CI 1, 7; P=0·003)	907:933	Multiple linear regression models adjusted for alcohol and smoking in trimester 1, showed that each additional 10 g/d CHO consumption was associated with an increase of 4 g (95 % CI 1, 7; P=0·003) in birth weight.
29316991	8	74	theme	g/d	981:983	arg1	intake					989:994	an additional 10 g/d fat intake	964:994	an additional 10 g/d fat intake	964:994	Conversely, an additional 10 g/d fat intake was associated with a lower birth weight of 8 g (95 % CI 0, 16; P=0·04) when we accounted for energy contributing macronutrients in each model, and maternal height, weight, parity, ethnicity, gestational age at delivery and sex of the baby.
29316991	6	75	theme	8-12	697:700	arg1	weeks					702:706	8-12 weeks	697:706	8-12 weeks	697:706	Dietary information was collected twice using a 24-h dietary recall about 8-12 weeks and 13-27 weeks of gestation.
29316991	10	76	theme	higher	1443:1448	arg1	weight					1456:1461	higher birth weight	1443:1461	higher birth weight of 52 g (95 % CI 4, 100; P=0·03) and 5 g (95 % CI 2, 7; P<0·001) respectively	1443:1539	Maternal diet in trimester 2 suggested that higher intakes of glucose (10 g/d) and lactose (1 g/d) were both associated with higher birth weight of 52 g (95 % CI 4, 100; P=0·03) and 5 g (95 % CI 2, 7; P<0·001) respectively.
29316991	0	77	theme	dietary	3:9	arg1	composition					25:35	dietary macronutrient composition	3:35	dietary macronutrient composition during pregnancy associated with offspring birth weight	3:91	Is dietary macronutrient composition during pregnancy associated with offspring birth weight?
29316991	4	78	with	women	452:456	arg1	pregnancies					473:483	singleton pregnancies	463:483	singleton pregnancies	463:483	This analyses included 1,196 women with singleton pregnancies who were part of the CAffeine and REproductive health study in Leeds, UK between 2003 and 2006.
29316991	7	79	theme	g	909:909	arg1	increase					895:902	an increase	892:902	an increase of 4 g (95 % CI 1, 7; P=0·003) in birth weight	892:949	Multiple linear regression models adjusted for alcohol and smoking in trimester 1, showed that each additional 10 g/d CHO consumption was associated with an increase of 4 g (95 % CI 1, 7; P=0·003) in birth weight.
29316991	2	80	theme	macronutrient	183:195	arg1	fats					231:234	fats	231:234	fats	231:234	There is lack of evidence on the differential impact of maternal macronutrient consumption: carbohydrates (CHO), fats and protein on birth weight.
29316991	2	80	theme	macronutrient	183:195	arg1	consumption					197:207	maternal macronutrient consumption: carbohydrates (CHO)	174:228	maternal macronutrient consumption: carbohydrates (CHO)	174:228	There is lack of evidence on the differential impact of maternal macronutrient consumption: carbohydrates (CHO), fats and protein on birth weight.
29316991	10	81	dep	%	1475:1475	arg1	P=0·03					1488:1493	P=0·03	1488:1493	P=0·03	1488:1493	Maternal diet in trimester 2 suggested that higher intakes of glucose (10 g/d) and lactose (1 g/d) were both associated with higher birth weight of 52 g (95 % CI 4, 100; P=0·03) and 5 g (95 % CI 2, 7; P<0·001) respectively.
29316991	6	82	theme	24-h	671:674	arg1	recall					684:689	a 24-h dietary recall	669:689	a 24-h dietary recall about 8-12 weeks and 13-27 weeks of gestation	669:735	Dietary information was collected twice using a 24-h dietary recall about 8-12 weeks and 13-27 weeks of gestation.
29316991	4	83	theme	study	539:543	arg1	part					494:497	part	494:497	part of the CAffeine and REproductive health study	494:543	This analyses included 1,196 women with singleton pregnancies who were part of the CAffeine and REproductive health study in Leeds, UK between 2003 and 2006.
29316991	11	84	theme	birth	1636:1640	arg1	outcomes					1649:1656	birth weight outcomes	1636:1656	birth weight outcomes	1636:1656	These results show that dietary macronutrient composition during pregnancy is associated with birth weight outcomes.
29316991	8	85	theme	birth	1024:1028	arg1	weight					1030:1035	a lower birth weight	1016:1035	a lower birth weight of 8 g	1016:1042	Conversely, an additional 10 g/d fat intake was associated with a lower birth weight of 8 g (95 % CI 0, 16; P=0·04) when we accounted for energy contributing macronutrients in each model, and maternal height, weight, parity, ethnicity, gestational age at delivery and sex of the baby.
29316991	3	86	theme	birth	409:413	arg1	weight					415:420	birth weight	409:420	birth weight	409:420	We investigated the association between maternal dietary macronutrient intakes and their sub-components such as saccharides and fatty acids and birth weight.
29316991	6	87	theme	gestation	727:735	arg1	weeks					702:706	8-12 weeks	697:706	8-12 weeks	697:706	Dietary information was collected twice using a 24-h dietary recall about 8-12 weeks and 13-27 weeks of gestation.
29316991	6	87	theme	gestation	727:735	arg1	weeks					718:722	13-27 weeks	712:722	13-27 weeks	712:722	Dietary information was collected twice using a 24-h dietary recall about 8-12 weeks and 13-27 weeks of gestation.
29316991	7	88	theme	regression	754:763	arg1	models					765:770	Multiple linear regression models	738:770	Multiple linear regression models	738:770	Multiple linear regression models adjusted for alcohol and smoking in trimester 1, showed that each additional 10 g/d CHO consumption was associated with an increase of 4 g (95 % CI 1, 7; P=0·003) in birth weight.
29316991	8	89	theme	g	1042:1042	arg1	weight					1030:1035	a lower birth weight	1016:1035	a lower birth weight of 8 g	1016:1042	Conversely, an additional 10 g/d fat intake was associated with a lower birth weight of 8 g (95 % CI 0, 16; P=0·04) when we accounted for energy contributing macronutrients in each model, and maternal height, weight, parity, ethnicity, gestational age at delivery and sex of the baby.
29316991	7	90	theme	Multiple	738:745	arg1	models					765:770	Multiple linear regression models	738:770	Multiple linear regression models	738:770	Multiple linear regression models adjusted for alcohol and smoking in trimester 1, showed that each additional 10 g/d CHO consumption was associated with an increase of 4 g (95 % CI 1, 7; P=0·003) in birth weight.
29281616	0	0	theme	fluid	71:75	arg1	composition					77:87	epithelial lining fluid composition	53:87	epithelial lining fluid composition in infants undergoing cardiac surgery with cardiopulmonary bypass	53:153	Influence of the type of congenital heart defects on epithelial lining fluid composition in infants undergoing cardiac surgery with cardiopulmonary bypass.
29281616	5	1	theme	surfactant	703:712	arg1	B					729:729	B	729:729	B (SP-A, SP-B)	729:742	We measured ELF phospholipids, surfactant proteins A and B (SP-A, SP-B), albumin, and myeloperoxidase activity.
29281616	5	1	theme	surfactant	703:712	arg1	A					723:723	A	723:723	A	723:723	We measured ELF phospholipids, surfactant proteins A and B (SP-A, SP-B), albumin, and myeloperoxidase activity.
29281616	5	1	theme	surfactant	703:712	arg1	proteins					714:721	surfactant proteins	703:721	surfactant proteins A and B (SP-A, SP-B)	703:742	We measured ELF phospholipids, surfactant proteins A and B (SP-A, SP-B), albumin, and myeloperoxidase activity.
29281616	7	2	located	found	1092:1096	arg2	levels					1068:1073	Increased levels	1058:1073	Increased levels of ELF SP-B	1058:1085	Increased levels of ELF SP-B were found in all defects, increased myeloperoxidase activity in all except the TOF, and increased levels of ELF albumin and SP-A only in ASD/VSD patients.
29281616	7	2	located	found	1092:1096	arg1	defects					1105:1111	all defects	1101:1111	all defects	1101:1111	Increased levels of ELF SP-B were found in all defects, increased myeloperoxidase activity in all except the TOF, and increased levels of ELF albumin and SP-A only in ASD/VSD patients.
29281616	1	3	theme	BackgroundIn	156:167	arg1	children					169:176	BackgroundIn children	156:176	BackgroundIn children with congenital heart disease (CHD), altered pulmonary circulation	156:243	BackgroundIn children with congenital heart disease (CHD), altered pulmonary circulation compromises gas exchange.
29281616	1	4	theme	gas	257:259	arg1	exchange					261:268	gas exchange	257:268	gas exchange	257:268	BackgroundIn children with congenital heart disease (CHD), altered pulmonary circulation compromises gas exchange.
29281616	0	5	theme	lining	64:69	arg1	fluid					71:75	epithelial lining fluid	53:75	epithelial lining fluid composition in infants undergoing cardiac surgery with cardiopulmonary bypass	53:153	Influence of the type of congenital heart defects on epithelial lining fluid composition in infants undergoing cardiac surgery with cardiopulmonary bypass.
29281616	3	6	from	effect	404:409	arg1	injury					437:442	lung injury	432:442	lung injury	432:442	No data are available on the effect of different CHDs on lung injury.
29281616	1	7	with	children	169:176	arg1	circulation					233:243	altered pulmonary circulation	215:243	altered pulmonary circulation	215:243	BackgroundIn children with congenital heart disease (CHD), altered pulmonary circulation compromises gas exchange.
29281616	1	7	with	children	169:176	arg1	CHD					209:211	CHD	209:211	CHD	209:211	BackgroundIn children with congenital heart disease (CHD), altered pulmonary circulation compromises gas exchange.
29281616	1	7	with	children	169:176	arg1	disease					200:206	congenital heart disease	183:206	congenital heart disease (CHD)	183:212	BackgroundIn children with congenital heart disease (CHD), altered pulmonary circulation compromises gas exchange.
29281616	6	8	with	infants	796:802	arg1	heart/lung					843:852	normal heart/lung	836:852	normal heart/lung served as controls.ResultsHeart defects	836:892	TAs from 12 infants (age 1.0 (0.9-2.9) months) with normal heart/lung served as controls.ResultsHeart defects were transposition of great arteries (19), tetralogy of Fallot (TOF, 20), atrial/ventricular septal defect (ASD/VSD, 22), and hypoplastic left heart syndrome (11).
29281616	6	9	from	infants	796:802	arg1	TAs					784:786	TAs	784:786	TAs from 12 infants (age 1.0 (0.9-2.9) months) with normal heart/lung served as controls.ResultsHeart defects	784:892	TAs from 12 infants (age 1.0 (0.9-2.9) months) with normal heart/lung served as controls.ResultsHeart defects were transposition of great arteries (19), tetralogy of Fallot (TOF, 20), atrial/ventricular septal defect (ASD/VSD, 22), and hypoplastic left heart syndrome (11).
29281616	0	10	from	Influence	0:8	arg1	composition					77:87	epithelial lining fluid composition	53:87	epithelial lining fluid composition in infants undergoing cardiac surgery with cardiopulmonary bypass	53:153	Influence of the type of congenital heart defects on epithelial lining fluid composition in infants undergoing cardiac surgery with cardiopulmonary bypass.
29281616	5	11	dep	SP-A	732:735	arg1	SP-B					738:741	SP-B	738:741	SP-B	738:741	We measured ELF phospholipids, surfactant proteins A and B (SP-A, SP-B), albumin, and myeloperoxidase activity.
29281616	4	12	with	children	538:545	arg1	aspirates					572:580	CHD.MethodsTracheal aspirates	552:580	CHD.MethodsTracheal aspirates (TAs) from 72 CHD children (age 2.9 (0.4-5.7) months)	552:634	The aim of this study was to analyze epithelial lining fluid (ELF) surfactant composition in children with CHD.MethodsTracheal aspirates (TAs) from 72 CHD children (age 2.9 (0.4-5.7) months) were obtained before and after CPB.
29281616	4	12	with	children	538:545	arg1	TAs					583:585	TAs	583:585	TAs	583:585	The aim of this study was to analyze epithelial lining fluid (ELF) surfactant composition in children with CHD.MethodsTracheal aspirates (TAs) from 72 CHD children (age 2.9 (0.4-5.7) months) were obtained before and after CPB.
29281616	7	13	theme	SP-A	1212:1215	arg1	levels					1186:1191	levels	1186:1191	levels of ELF albumin and SP-A	1186:1215	Increased levels of ELF SP-B were found in all defects, increased myeloperoxidase activity in all except the TOF, and increased levels of ELF albumin and SP-A only in ASD/VSD patients.
29281616	8	14	from	patterns	1392:1399	arg1	CHD					1414:1416	different CHD	1404:1416	different CHD	1404:1416	Postoperatively, ELF findings remained unchanged except for a further increase in myeloperoxidase activity.ConclusionELF composition has distinctive patterns in different CHD.
29281616	6	15	theme	hypoplastic	1020:1030	arg1	11					1053:1054	11	1053:1054	11	1053:1054	TAs from 12 infants (age 1.0 (0.9-2.9) months) with normal heart/lung served as controls.ResultsHeart defects were transposition of great arteries (19), tetralogy of Fallot (TOF, 20), atrial/ventricular septal defect (ASD/VSD, 22), and hypoplastic left heart syndrome (11).
29281616	6	15	theme	hypoplastic	1020:1030	arg1	syndrome					1043:1050	hypoplastic left heart syndrome	1020:1050	hypoplastic left heart syndrome (11)	1020:1055	TAs from 12 infants (age 1.0 (0.9-2.9) months) with normal heart/lung served as controls.ResultsHeart defects were transposition of great arteries (19), tetralogy of Fallot (TOF, 20), atrial/ventricular septal defect (ASD/VSD, 22), and hypoplastic left heart syndrome (11).
29281616	6	16	theme	controls.ResultsHeart	864:884	arg1	defects					886:892	controls.ResultsHeart defects	864:892	controls.ResultsHeart defects	864:892	TAs from 12 infants (age 1.0 (0.9-2.9) months) with normal heart/lung served as controls.ResultsHeart defects were transposition of great arteries (19), tetralogy of Fallot (TOF, 20), atrial/ventricular septal defect (ASD/VSD, 22), and hypoplastic left heart syndrome (11).
29281616	8	17	theme	activity.ConclusionELF	1341:1362	arg1	composition					1364:1374	myeloperoxidase activity.ConclusionELF composition	1325:1374	myeloperoxidase activity.ConclusionELF composition has distinctive patterns in different CHD	1325:1416	Postoperatively, ELF findings remained unchanged except for a further increase in myeloperoxidase activity.ConclusionELF composition has distinctive patterns in different CHD.
29281616	7	18	theme	albumin	1200:1206	arg1	levels					1186:1191	levels	1186:1191	levels of ELF albumin and SP-A	1186:1215	Increased levels of ELF SP-B were found in all defects, increased myeloperoxidase activity in all except the TOF, and increased levels of ELF albumin and SP-A only in ASD/VSD patients.
29281616	2	19	theme	pulmonary	281:289	arg1	dysfunction					291:301	pulmonary dysfunction	281:301	pulmonary dysfunction	281:301	Moreover, pulmonary dysfunction is a complication of cardiac surgery with cardiopulmonary bypass (CPB).
29281616	2	19	theme	pulmonary	281:289	arg1	complication					308:319	a complication	306:319	a complication of cardiac surgery with cardiopulmonary bypass (CPB)	306:372	Moreover, pulmonary dysfunction is a complication of cardiac surgery with cardiopulmonary bypass (CPB).
29281616	3	20	theme	different	414:422	arg1	CHDs					424:427	different CHDs	414:427	different CHDs	414:427	No data are available on the effect of different CHDs on lung injury.
29281616	5	21	theme	myeloperoxidase	758:772	arg1	activity					774:781	myeloperoxidase activity	758:781	myeloperoxidase activity	758:781	We measured ELF phospholipids, surfactant proteins A and B (SP-A, SP-B), albumin, and myeloperoxidase activity.
29281616	5	22	theme	ELF	684:686	arg1	phospholipids					688:700	ELF phospholipids	684:700	ELF phospholipids	684:700	We measured ELF phospholipids, surfactant proteins A and B (SP-A, SP-B), albumin, and myeloperoxidase activity.
29281616	8	23	theme	distinctive	1380:1390	arg1	patterns					1392:1399	distinctive patterns	1380:1399	distinctive patterns in different CHD	1380:1416	Postoperatively, ELF findings remained unchanged except for a further increase in myeloperoxidase activity.ConclusionELF composition has distinctive patterns in different CHD.
29281616	4	24	theme	CHD.MethodsTracheal	552:570	arg1	aspirates					572:580	CHD.MethodsTracheal aspirates	552:580	CHD.MethodsTracheal aspirates (TAs) from 72 CHD children (age 2.9 (0.4-5.7) months)	552:634	The aim of this study was to analyze epithelial lining fluid (ELF) surfactant composition in children with CHD.MethodsTracheal aspirates (TAs) from 72 CHD children (age 2.9 (0.4-5.7) months) were obtained before and after CPB.
29281616	4	24	theme	CHD.MethodsTracheal	552:570	arg1	TAs					583:585	TAs	583:585	TAs	583:585	The aim of this study was to analyze epithelial lining fluid (ELF) surfactant composition in children with CHD.MethodsTracheal aspirates (TAs) from 72 CHD children (age 2.9 (0.4-5.7) months) were obtained before and after CPB.
29281616	6	25	theme	arteries	922:929	arg1	11					1053:1054	11	1053:1054	11	1053:1054	TAs from 12 infants (age 1.0 (0.9-2.9) months) with normal heart/lung served as controls.ResultsHeart defects were transposition of great arteries (19), tetralogy of Fallot (TOF, 20), atrial/ventricular septal defect (ASD/VSD, 22), and hypoplastic left heart syndrome (11).
29281616	6	25	theme	arteries	922:929	arg1	transposition					899:911	transposition	899:911	transposition of great arteries (19)	899:934	TAs from 12 infants (age 1.0 (0.9-2.9) months) with normal heart/lung served as controls.ResultsHeart defects were transposition of great arteries (19), tetralogy of Fallot (TOF, 20), atrial/ventricular septal defect (ASD/VSD, 22), and hypoplastic left heart syndrome (11).
29281616	6	25	theme	arteries	922:929	arg1	defect					994:999	atrial/ventricular septal defect	968:999	atrial/ventricular septal defect (ASD/VSD, 22)	968:1013	TAs from 12 infants (age 1.0 (0.9-2.9) months) with normal heart/lung served as controls.ResultsHeart defects were transposition of great arteries (19), tetralogy of Fallot (TOF, 20), atrial/ventricular septal defect (ASD/VSD, 22), and hypoplastic left heart syndrome (11).
29281616	6	25	theme	arteries	922:929	arg1	syndrome					1043:1050	hypoplastic left heart syndrome	1020:1050	hypoplastic left heart syndrome (11)	1020:1055	TAs from 12 infants (age 1.0 (0.9-2.9) months) with normal heart/lung served as controls.ResultsHeart defects were transposition of great arteries (19), tetralogy of Fallot (TOF, 20), atrial/ventricular septal defect (ASD/VSD, 22), and hypoplastic left heart syndrome (11).
29281616	6	25	theme	arteries	922:929	arg1	tetralogy					937:945	tetralogy	937:945	tetralogy of Fallot (TOF, 20)	937:965	TAs from 12 infants (age 1.0 (0.9-2.9) months) with normal heart/lung served as controls.ResultsHeart defects were transposition of great arteries (19), tetralogy of Fallot (TOF, 20), atrial/ventricular septal defect (ASD/VSD, 22), and hypoplastic left heart syndrome (11).
29281616	1	26	theme	congenital	183:192	arg1	circulation					233:243	altered pulmonary circulation	215:243	altered pulmonary circulation	215:243	BackgroundIn children with congenital heart disease (CHD), altered pulmonary circulation compromises gas exchange.
29281616	1	26	theme	congenital	183:192	arg1	CHD					209:211	CHD	209:211	CHD	209:211	BackgroundIn children with congenital heart disease (CHD), altered pulmonary circulation compromises gas exchange.
29281616	1	26	theme	congenital	183:192	arg1	disease					200:206	congenital heart disease	183:206	congenital heart disease (CHD)	183:212	BackgroundIn children with congenital heart disease (CHD), altered pulmonary circulation compromises gas exchange.
29281616	4	27	theme	study	461:465	arg1	aim					449:451	The aim	445:451	The aim of this study	445:465	The aim of this study was to analyze epithelial lining fluid (ELF) surfactant composition in children with CHD.MethodsTracheal aspirates (TAs) from 72 CHD children (age 2.9 (0.4-5.7) months) were obtained before and after CPB.
29281616	7	28	theme	Increased	1058:1066	arg1	levels					1068:1073	Increased levels	1058:1073	Increased levels of ELF SP-B	1058:1085	Increased levels of ELF SP-B were found in all defects, increased myeloperoxidase activity in all except the TOF, and increased levels of ELF albumin and SP-A only in ASD/VSD patients.
29281616	2	29	theme	cardiac	324:330	arg1	surgery					332:338	cardiac surgery	324:338	cardiac surgery with cardiopulmonary bypass (CPB)	324:372	Moreover, pulmonary dysfunction is a complication of cardiac surgery with cardiopulmonary bypass (CPB).
29281616	1	30	theme	heart	194:198	arg1	circulation					233:243	altered pulmonary circulation	215:243	altered pulmonary circulation	215:243	BackgroundIn children with congenital heart disease (CHD), altered pulmonary circulation compromises gas exchange.
29281616	1	30	theme	heart	194:198	arg1	CHD					209:211	CHD	209:211	CHD	209:211	BackgroundIn children with congenital heart disease (CHD), altered pulmonary circulation compromises gas exchange.
29281616	1	30	theme	heart	194:198	arg1	disease					200:206	congenital heart disease	183:206	congenital heart disease (CHD)	183:212	BackgroundIn children with congenital heart disease (CHD), altered pulmonary circulation compromises gas exchange.
29281616	4	31	theme	lining	493:498	arg1	ELF					507:509	ELF	507:509	ELF	507:509	The aim of this study was to analyze epithelial lining fluid (ELF) surfactant composition in children with CHD.MethodsTracheal aspirates (TAs) from 72 CHD children (age 2.9 (0.4-5.7) months) were obtained before and after CPB.
29281616	4	31	theme	lining	493:498	arg1	fluid					500:504	epithelial lining fluid	482:504	epithelial lining fluid (ELF)	482:510	The aim of this study was to analyze epithelial lining fluid (ELF) surfactant composition in children with CHD.MethodsTracheal aspirates (TAs) from 72 CHD children (age 2.9 (0.4-5.7) months) were obtained before and after CPB.
29281616	9	32	theme	ELF	1463:1465	arg1	changes					1479:1485	the ELF biochemical changes	1459:1485	the ELF biochemical changes	1459:1485	We speculate that a better knowledge of the ELF biochemical changes may help to prevent respiratory complications.
29281616	6	33	theme	22	1011:1012	arg1	ASD/VSD					1002:1008	ASD/VSD	1002:1008	ASD/VSD	1002:1008	TAs from 12 infants (age 1.0 (0.9-2.9) months) with normal heart/lung served as controls.ResultsHeart defects were transposition of great arteries (19), tetralogy of Fallot (TOF, 20), atrial/ventricular septal defect (ASD/VSD, 22), and hypoplastic left heart syndrome (11).
29281616	3	34	theme	CHDs	424:427	arg1	effect					404:409	the effect	400:409	the effect of different CHDs on lung injury	400:442	No data are available on the effect of different CHDs on lung injury.
29281616	0	35	theme	type	17:20	arg1	Influence					0:8	Influence	0:8	Influence of the type of congenital heart defects on epithelial lining fluid composition in infants undergoing cardiac surgery with cardiopulmonary bypass	0:153	Influence of the type of congenital heart defects on epithelial lining fluid composition in infants undergoing cardiac surgery with cardiopulmonary bypass.
29281616	4	36	theme	surfactant	512:521	arg1	composition					523:533	surfactant composition	512:533	surfactant composition in children with CHD.MethodsTracheal aspirates (TAs) from 72 CHD children (age 2.9 (0.4-5.7) months)	512:634	The aim of this study was to analyze epithelial lining fluid (ELF) surfactant composition in children with CHD.MethodsTracheal aspirates (TAs) from 72 CHD children (age 2.9 (0.4-5.7) months) were obtained before and after CPB.
29281616	7	37	theme	ELF	1196:1198	arg1	albumin					1200:1206	ELF albumin	1196:1206	ELF albumin	1196:1206	Increased levels of ELF SP-B were found in all defects, increased myeloperoxidase activity in all except the TOF, and increased levels of ELF albumin and SP-A only in ASD/VSD patients.
29281616	7	38	theme	myeloperoxidase	1124:1138	arg1	activity					1140:1147	myeloperoxidase activity	1124:1147	myeloperoxidase activity	1124:1147	Increased levels of ELF SP-B were found in all defects, increased myeloperoxidase activity in all except the TOF, and increased levels of ELF albumin and SP-A only in ASD/VSD patients.
29281616	6	39	theme	atrial/ventricular	968:985	arg1	defect					994:999	atrial/ventricular septal defect	968:999	atrial/ventricular septal defect (ASD/VSD, 22)	968:1013	TAs from 12 infants (age 1.0 (0.9-2.9) months) with normal heart/lung served as controls.ResultsHeart defects were transposition of great arteries (19), tetralogy of Fallot (TOF, 20), atrial/ventricular septal defect (ASD/VSD, 22), and hypoplastic left heart syndrome (11).
29281616	0	40	theme	cardiac	111:117	arg1	surgery					119:125	cardiac surgery	111:125	cardiac surgery	111:125	Influence of the type of congenital heart defects on epithelial lining fluid composition in infants undergoing cardiac surgery with cardiopulmonary bypass.
29281616	8	41	theme	ELF	1260:1262	arg1	findings					1264:1271	ELF findings	1260:1271	ELF findings	1260:1271	Postoperatively, ELF findings remained unchanged except for a further increase in myeloperoxidase activity.ConclusionELF composition has distinctive patterns in different CHD.
29281616	7	42	theme	ELF	1078:1080	arg1	SP-B					1082:1085	ELF SP-B	1078:1085	ELF SP-B	1078:1085	Increased levels of ELF SP-B were found in all defects, increased myeloperoxidase activity in all except the TOF, and increased levels of ELF albumin and SP-A only in ASD/VSD patients.
29281616	4	43	from	children	600:607	arg1	aspirates					572:580	CHD.MethodsTracheal aspirates	552:580	CHD.MethodsTracheal aspirates (TAs) from 72 CHD children (age 2.9 (0.4-5.7) months)	552:634	The aim of this study was to analyze epithelial lining fluid (ELF) surfactant composition in children with CHD.MethodsTracheal aspirates (TAs) from 72 CHD children (age 2.9 (0.4-5.7) months) were obtained before and after CPB.
29281616	4	43	from	children	600:607	arg1	TAs					583:585	TAs	583:585	TAs	583:585	The aim of this study was to analyze epithelial lining fluid (ELF) surfactant composition in children with CHD.MethodsTracheal aspirates (TAs) from 72 CHD children (age 2.9 (0.4-5.7) months) were obtained before and after CPB.
29281616	4	44	theme	CHD	596:598	arg1	months					628:633	age 2.9 (0.4-5.7) months	610:633	age 2.9 (0.4-5.7) months	610:633	The aim of this study was to analyze epithelial lining fluid (ELF) surfactant composition in children with CHD.MethodsTracheal aspirates (TAs) from 72 CHD children (age 2.9 (0.4-5.7) months) were obtained before and after CPB.
29281616	4	44	theme	CHD	596:598	arg1	children					600:607	72 CHD children	593:607	72 CHD children (age 2.9 (0.4-5.7) months)	593:634	The aim of this study was to analyze epithelial lining fluid (ELF) surfactant composition in children with CHD.MethodsTracheal aspirates (TAs) from 72 CHD children (age 2.9 (0.4-5.7) months) were obtained before and after CPB.
29281616	0	45	theme	congenital	25:34	arg1	defects					42:48	congenital heart defects	25:48	congenital heart defects	25:48	Influence of the type of congenital heart defects on epithelial lining fluid composition in infants undergoing cardiac surgery with cardiopulmonary bypass.
29281616	5	46	dep	proteins	714:721	arg1	B					729:729	B	729:729	B (SP-A, SP-B)	729:742	We measured ELF phospholipids, surfactant proteins A and B (SP-A, SP-B), albumin, and myeloperoxidase activity.
29281616	5	46	dep	proteins	714:721	arg1	A					723:723	A	723:723	A	723:723	We measured ELF phospholipids, surfactant proteins A and B (SP-A, SP-B), albumin, and myeloperoxidase activity.
29281616	5	46	dep	proteins	714:721	arg1	SP-A					732:735	SP-A	732:735	SP-A	732:735	We measured ELF phospholipids, surfactant proteins A and B (SP-A, SP-B), albumin, and myeloperoxidase activity.
29281616	5	46	dep	proteins	714:721	arg1	proteins					714:721	surfactant proteins	703:721	surfactant proteins A and B (SP-A, SP-B)	703:742	We measured ELF phospholipids, surfactant proteins A and B (SP-A, SP-B), albumin, and myeloperoxidase activity.
29281616	2	47	with	surgery	332:338	arg1	CPB					369:371	CPB	369:371	CPB	369:371	Moreover, pulmonary dysfunction is a complication of cardiac surgery with cardiopulmonary bypass (CPB).
29281616	2	47	with	surgery	332:338	arg1	bypass					361:366	bypass	361:366	bypass	361:366	Moreover, pulmonary dysfunction is a complication of cardiac surgery with cardiopulmonary bypass (CPB).
29281616	6	48	theme	normal	836:841	arg1	heart/lung					843:852	normal heart/lung	836:852	normal heart/lung served as controls.ResultsHeart defects	836:892	TAs from 12 infants (age 1.0 (0.9-2.9) months) with normal heart/lung served as controls.ResultsHeart defects were transposition of great arteries (19), tetralogy of Fallot (TOF, 20), atrial/ventricular septal defect (ASD/VSD, 22), and hypoplastic left heart syndrome (11).
29281616	4	49	theme	age	610:612	arg1	months					628:633	age 2.9 (0.4-5.7) months	610:633	age 2.9 (0.4-5.7) months	610:633	The aim of this study was to analyze epithelial lining fluid (ELF) surfactant composition in children with CHD.MethodsTracheal aspirates (TAs) from 72 CHD children (age 2.9 (0.4-5.7) months) were obtained before and after CPB.
29281616	4	49	theme	age	610:612	arg1	children					600:607	72 CHD children	593:607	72 CHD children (age 2.9 (0.4-5.7) months)	593:634	The aim of this study was to analyze epithelial lining fluid (ELF) surfactant composition in children with CHD.MethodsTracheal aspirates (TAs) from 72 CHD children (age 2.9 (0.4-5.7) months) were obtained before and after CPB.
29281616	4	50	from	composition	523:533	arg1	children					538:545	children	538:545	children with CHD.MethodsTracheal aspirates (TAs) from 72 CHD children (age 2.9 (0.4-5.7) months)	538:634	The aim of this study was to analyze epithelial lining fluid (ELF) surfactant composition in children with CHD.MethodsTracheal aspirates (TAs) from 72 CHD children (age 2.9 (0.4-5.7) months) were obtained before and after CPB.
29281616	6	51	theme	Fallot	950:955	arg1	11					1053:1054	11	1053:1054	11	1053:1054	TAs from 12 infants (age 1.0 (0.9-2.9) months) with normal heart/lung served as controls.ResultsHeart defects were transposition of great arteries (19), tetralogy of Fallot (TOF, 20), atrial/ventricular septal defect (ASD/VSD, 22), and hypoplastic left heart syndrome (11).
29281616	6	51	theme	Fallot	950:955	arg1	transposition					899:911	transposition	899:911	transposition of great arteries (19)	899:934	TAs from 12 infants (age 1.0 (0.9-2.9) months) with normal heart/lung served as controls.ResultsHeart defects were transposition of great arteries (19), tetralogy of Fallot (TOF, 20), atrial/ventricular septal defect (ASD/VSD, 22), and hypoplastic left heart syndrome (11).
29281616	6	51	theme	Fallot	950:955	arg1	defect					994:999	atrial/ventricular septal defect	968:999	atrial/ventricular septal defect (ASD/VSD, 22)	968:1013	TAs from 12 infants (age 1.0 (0.9-2.9) months) with normal heart/lung served as controls.ResultsHeart defects were transposition of great arteries (19), tetralogy of Fallot (TOF, 20), atrial/ventricular septal defect (ASD/VSD, 22), and hypoplastic left heart syndrome (11).
29281616	6	51	theme	Fallot	950:955	arg1	syndrome					1043:1050	hypoplastic left heart syndrome	1020:1050	hypoplastic left heart syndrome (11)	1020:1055	TAs from 12 infants (age 1.0 (0.9-2.9) months) with normal heart/lung served as controls.ResultsHeart defects were transposition of great arteries (19), tetralogy of Fallot (TOF, 20), atrial/ventricular septal defect (ASD/VSD, 22), and hypoplastic left heart syndrome (11).
29281616	6	51	theme	Fallot	950:955	arg1	tetralogy					937:945	tetralogy	937:945	tetralogy of Fallot (TOF, 20)	937:965	TAs from 12 infants (age 1.0 (0.9-2.9) months) with normal heart/lung served as controls.ResultsHeart defects were transposition of great arteries (19), tetralogy of Fallot (TOF, 20), atrial/ventricular septal defect (ASD/VSD, 22), and hypoplastic left heart syndrome (11).
29281616	0	52	theme	defects	42:48	arg1	type					17:20	the type	13:20	the type of congenital heart defects	13:48	Influence of the type of congenital heart defects on epithelial lining fluid composition in infants undergoing cardiac surgery with cardiopulmonary bypass.
29281616	9	53	theme	better	1439:1444	arg1	knowledge					1446:1454	a better knowledge	1437:1454	a better knowledge of the ELF biochemical changes	1437:1485	We speculate that a better knowledge of the ELF biochemical changes may help to prevent respiratory complications.
29281616	8	54	theme	further	1305:1311	arg1	increase					1313:1320	a further increase	1303:1320	a further increase in myeloperoxidase activity.ConclusionELF composition has distinctive patterns in different CHD	1303:1416	Postoperatively, ELF findings remained unchanged except for a further increase in myeloperoxidase activity.ConclusionELF composition has distinctive patterns in different CHD.
29281616	0	55	theme	heart	36:40	arg1	defects					42:48	congenital heart defects	25:48	congenital heart defects	25:48	Influence of the type of congenital heart defects on epithelial lining fluid composition in infants undergoing cardiac surgery with cardiopulmonary bypass.
29281616	4	56	dep	analyze	474:480	arg1	obtained					641:648	obtained	641:648	were obtained before and after CPB	636:669	The aim of this study was to analyze epithelial lining fluid (ELF) surfactant composition in children with CHD.MethodsTracheal aspirates (TAs) from 72 CHD children (age 2.9 (0.4-5.7) months) were obtained before and after CPB.
29281616	8	57	theme	different	1404:1412	arg1	CHD					1414:1416	different CHD	1404:1416	different CHD	1404:1416	Postoperatively, ELF findings remained unchanged except for a further increase in myeloperoxidase activity.ConclusionELF composition has distinctive patterns in different CHD.
29281616	6	58	theme	great	916:920	arg1	19					932:933	19	932:933	19	932:933	TAs from 12 infants (age 1.0 (0.9-2.9) months) with normal heart/lung served as controls.ResultsHeart defects were transposition of great arteries (19), tetralogy of Fallot (TOF, 20), atrial/ventricular septal defect (ASD/VSD, 22), and hypoplastic left heart syndrome (11).
29281616	6	58	theme	great	916:920	arg1	arteries					922:929	great arteries	916:929	great arteries (19)	916:934	TAs from 12 infants (age 1.0 (0.9-2.9) months) with normal heart/lung served as controls.ResultsHeart defects were transposition of great arteries (19), tetralogy of Fallot (TOF, 20), atrial/ventricular septal defect (ASD/VSD, 22), and hypoplastic left heart syndrome (11).
29281616	6	59	dep	defect	994:999	arg1	ASD/VSD					1002:1008	ASD/VSD	1002:1008	ASD/VSD	1002:1008	TAs from 12 infants (age 1.0 (0.9-2.9) months) with normal heart/lung served as controls.ResultsHeart defects were transposition of great arteries (19), tetralogy of Fallot (TOF, 20), atrial/ventricular septal defect (ASD/VSD, 22), and hypoplastic left heart syndrome (11).
29281616	8	60	dep	composition	1364:1374	arg1	has					1376:1378	has	1376:1378	has distinctive patterns in different CHD	1376:1416	Postoperatively, ELF findings remained unchanged except for a further increase in myeloperoxidase activity.ConclusionELF composition has distinctive patterns in different CHD.
29281616	7	61	theme	ASD/VSD	1225:1231	arg1	patients					1233:1240	ASD/VSD patients	1225:1240	ASD/VSD patients	1225:1240	Increased levels of ELF SP-B were found in all defects, increased myeloperoxidase activity in all except the TOF, and increased levels of ELF albumin and SP-A only in ASD/VSD patients.
29281616	6	62	theme	septal	987:992	arg1	defect					994:999	atrial/ventricular septal defect	968:999	atrial/ventricular septal defect (ASD/VSD, 22)	968:1013	TAs from 12 infants (age 1.0 (0.9-2.9) months) with normal heart/lung served as controls.ResultsHeart defects were transposition of great arteries (19), tetralogy of Fallot (TOF, 20), atrial/ventricular septal defect (ASD/VSD, 22), and hypoplastic left heart syndrome (11).
29281616	1	63	theme	altered	215:221	arg1	circulation					233:243	altered pulmonary circulation	215:243	altered pulmonary circulation	215:243	BackgroundIn children with congenital heart disease (CHD), altered pulmonary circulation compromises gas exchange.
29281616	1	63	theme	altered	215:221	arg1	disease					200:206	congenital heart disease	183:206	congenital heart disease (CHD)	183:212	BackgroundIn children with congenital heart disease (CHD), altered pulmonary circulation compromises gas exchange.
29281616	4	64	theme	epithelial	482:491	arg1	ELF					507:509	ELF	507:509	ELF	507:509	The aim of this study was to analyze epithelial lining fluid (ELF) surfactant composition in children with CHD.MethodsTracheal aspirates (TAs) from 72 CHD children (age 2.9 (0.4-5.7) months) were obtained before and after CPB.
29281616	4	64	theme	epithelial	482:491	arg1	fluid					500:504	epithelial lining fluid	482:504	epithelial lining fluid (ELF)	482:510	The aim of this study was to analyze epithelial lining fluid (ELF) surfactant composition in children with CHD.MethodsTracheal aspirates (TAs) from 72 CHD children (age 2.9 (0.4-5.7) months) were obtained before and after CPB.
29281616	6	65	theme	left	1032:1035	arg1	11					1053:1054	11	1053:1054	11	1053:1054	TAs from 12 infants (age 1.0 (0.9-2.9) months) with normal heart/lung served as controls.ResultsHeart defects were transposition of great arteries (19), tetralogy of Fallot (TOF, 20), atrial/ventricular septal defect (ASD/VSD, 22), and hypoplastic left heart syndrome (11).
29281616	6	65	theme	left	1032:1035	arg1	syndrome					1043:1050	hypoplastic left heart syndrome	1020:1050	hypoplastic left heart syndrome (11)	1020:1055	TAs from 12 infants (age 1.0 (0.9-2.9) months) with normal heart/lung served as controls.ResultsHeart defects were transposition of great arteries (19), tetralogy of Fallot (TOF, 20), atrial/ventricular septal defect (ASD/VSD, 22), and hypoplastic left heart syndrome (11).
29281616	8	66	from	increase	1313:1320	arg1	composition					1364:1374	myeloperoxidase activity.ConclusionELF composition	1325:1374	myeloperoxidase activity.ConclusionELF composition has distinctive patterns in different CHD	1325:1416	Postoperatively, ELF findings remained unchanged except for a further increase in myeloperoxidase activity.ConclusionELF composition has distinctive patterns in different CHD.
29281616	2	67	theme	surgery	332:338	arg1	dysfunction					291:301	pulmonary dysfunction	281:301	pulmonary dysfunction	281:301	Moreover, pulmonary dysfunction is a complication of cardiac surgery with cardiopulmonary bypass (CPB).
29281616	2	67	theme	surgery	332:338	arg1	complication					308:319	a complication	306:319	a complication of cardiac surgery with cardiopulmonary bypass (CPB)	306:372	Moreover, pulmonary dysfunction is a complication of cardiac surgery with cardiopulmonary bypass (CPB).
29281616	1	68	theme	pulmonary	223:231	arg1	circulation					233:243	altered pulmonary circulation	215:243	altered pulmonary circulation	215:243	BackgroundIn children with congenital heart disease (CHD), altered pulmonary circulation compromises gas exchange.
29281616	1	68	theme	pulmonary	223:231	arg1	disease					200:206	congenital heart disease	183:206	congenital heart disease (CHD)	183:212	BackgroundIn children with congenital heart disease (CHD), altered pulmonary circulation compromises gas exchange.
29281616	0	69	theme	epithelial	53:62	arg1	fluid					71:75	epithelial lining fluid	53:75	epithelial lining fluid composition in infants undergoing cardiac surgery with cardiopulmonary bypass	53:153	Influence of the type of congenital heart defects on epithelial lining fluid composition in infants undergoing cardiac surgery with cardiopulmonary bypass.
29281616	0	70	from	composition	77:87	arg1	infants					92:98	infants	92:98	infants undergoing cardiac surgery with cardiopulmonary bypass	92:153	Influence of the type of congenital heart defects on epithelial lining fluid composition in infants undergoing cardiac surgery with cardiopulmonary bypass.
29281616	9	71	theme	biochemical	1467:1477	arg1	changes					1479:1485	the ELF biochemical changes	1459:1485	the ELF biochemical changes	1459:1485	We speculate that a better knowledge of the ELF biochemical changes may help to prevent respiratory complications.
29281616	7	72	theme	SP-B	1082:1085	arg1	levels					1068:1073	Increased levels	1058:1073	Increased levels of ELF SP-B	1058:1085	Increased levels of ELF SP-B were found in all defects, increased myeloperoxidase activity in all except the TOF, and increased levels of ELF albumin and SP-A only in ASD/VSD patients.
29281616	3	73	theme	lung	432:435	arg1	injury					437:442	lung injury	432:442	lung injury	432:442	No data are available on the effect of different CHDs on lung injury.
29281616	6	74	theme	age	805:807	arg1	infants					796:802	12 infants	793:802	12 infants (age 1.0 (0.9-2.9) months) with normal heart/lung served as controls.ResultsHeart defects	793:892	TAs from 12 infants (age 1.0 (0.9-2.9) months) with normal heart/lung served as controls.ResultsHeart defects were transposition of great arteries (19), tetralogy of Fallot (TOF, 20), atrial/ventricular septal defect (ASD/VSD, 22), and hypoplastic left heart syndrome (11).
29281616	6	74	theme	age	805:807	arg1	months					823:828	age 1.0 (0.9-2.9) months	805:828	age 1.0 (0.9-2.9) months	805:828	TAs from 12 infants (age 1.0 (0.9-2.9) months) with normal heart/lung served as controls.ResultsHeart defects were transposition of great arteries (19), tetralogy of Fallot (TOF, 20), atrial/ventricular septal defect (ASD/VSD, 22), and hypoplastic left heart syndrome (11).
29281616	9	75	theme	respiratory	1507:1517	arg1	complications					1519:1531	respiratory complications	1507:1531	respiratory complications	1507:1531	We speculate that a better knowledge of the ELF biochemical changes may help to prevent respiratory complications.
29281616	6	76	theme	heart	1037:1041	arg1	11					1053:1054	11	1053:1054	11	1053:1054	TAs from 12 infants (age 1.0 (0.9-2.9) months) with normal heart/lung served as controls.ResultsHeart defects were transposition of great arteries (19), tetralogy of Fallot (TOF, 20), atrial/ventricular septal defect (ASD/VSD, 22), and hypoplastic left heart syndrome (11).
29281616	6	76	theme	heart	1037:1041	arg1	syndrome					1043:1050	hypoplastic left heart syndrome	1020:1050	hypoplastic left heart syndrome (11)	1020:1055	TAs from 12 infants (age 1.0 (0.9-2.9) months) with normal heart/lung served as controls.ResultsHeart defects were transposition of great arteries (19), tetralogy of Fallot (TOF, 20), atrial/ventricular septal defect (ASD/VSD, 22), and hypoplastic left heart syndrome (11).
29281616	8	77	theme	myeloperoxidase	1325:1339	arg1	composition					1364:1374	myeloperoxidase activity.ConclusionELF composition	1325:1374	myeloperoxidase activity.ConclusionELF composition has distinctive patterns in different CHD	1325:1416	Postoperatively, ELF findings remained unchanged except for a further increase in myeloperoxidase activity.ConclusionELF composition has distinctive patterns in different CHD.
29281616	9	78	theme	changes	1479:1485	arg1	knowledge					1446:1454	a better knowledge	1437:1454	a better knowledge of the ELF biochemical changes	1437:1485	We speculate that a better knowledge of the ELF biochemical changes may help to prevent respiratory complications.
31063121	2	0	theme	Gram-stain-positive	104:122	arg1	bacterium					164:172	A Gram-stain-positive, aerobic, rod-shaped, endospore-forming bacterium	102:172	A Gram-stain-positive, aerobic, rod-shaped, endospore-forming bacterium	102:172	A Gram-stain-positive, aerobic, rod-shaped, endospore-forming bacterium, designated strain M2MS4P-1T, was isolated from surface-sterilized bark of Sonneratiaapetala sampled in Guangxi, China.
31063121	5	1	theme	type	653:656	arg1	strain					658:663	the type strain	649:663	the type strain of C. luojiensis HY-22RT	649:688	The average nucleotide identity value and estimated DDH value between strain M2MS4P-1T and the type strain of C. luojiensis HY-22RT were 79.2 and 20.1 %, respectively.
31063121	8	2	theme	strain	1017:1022	arg1	M2MS4P-1T					1024:1032	strain M2MS4P-1T	1017:1032	strain M2MS4P-1T	1017:1032	The predominant isoprenoid quinone in strain M2MS4P-1T was menaquinone-7.
31063121	12	3	theme	strain	1419:1424	arg1	M2MS4P-1T					1426:1434	strain M2MS4P-1T	1419:1434	strain M2MS4P-1T	1419:1434	According to the phylogenetic, phenotypic and chemotaxonomic evidence, strain M2MS4P-1T was clearly distinguishable from other species with validly published names in the genus Cohnella and should therefore be classified as a novel species, for which we suggest the name Cohnellaendophytica sp.
31063121	12	3	theme	strain	1419:1424	arg1	species					1580:1586	a novel species	1572:1586	a novel species	1572:1586	According to the phylogenetic, phenotypic and chemotaxonomic evidence, strain M2MS4P-1T was clearly distinguishable from other species with validly published names in the genus Cohnella and should therefore be classified as a novel species, for which we suggest the name Cohnellaendophytica sp.
31063121	4	4	theme	sequence	402:409	arg1	comparisons					411:421	16S rRNA gene sequence comparisons	388:421	16S rRNA gene sequence comparisons	388:421	16S rRNA gene sequence comparisons revealed that strain M2MS4P-1T belonged to the genus Cohnella and was most closely to Cohnella luojiensis HY-22RT (98.4 % similarity).
31063121	12	5	theme	published	1496:1504	arg1	names					1506:1510	validly published names	1488:1510	validly published names in the genus Cohnella	1488:1532	According to the phylogenetic, phenotypic and chemotaxonomic evidence, strain M2MS4P-1T was clearly distinguishable from other species with validly published names in the genus Cohnella and should therefore be classified as a novel species, for which we suggest the name Cohnellaendophytica sp.
31063121	12	6	with	species	1475:1481	arg1	names					1506:1510	validly published names	1488:1510	validly published names in the genus Cohnella	1488:1532	According to the phylogenetic, phenotypic and chemotaxonomic evidence, strain M2MS4P-1T was clearly distinguishable from other species with validly published names in the genus Cohnella and should therefore be classified as a novel species, for which we suggest the name Cohnellaendophytica sp.
31063121	4	7	theme	gene	397:400	arg1	comparisons					411:421	16S rRNA gene sequence comparisons	388:421	16S rRNA gene sequence comparisons	388:421	16S rRNA gene sequence comparisons revealed that strain M2MS4P-1T belonged to the genus Cohnella and was most closely to Cohnella luojiensis HY-22RT (98.4 % similarity).
31063121	1	8	theme	endophytic	39:48	arg1	bacterium					50:58	a novel endophytic bacterium	31:58	a novel endophytic bacterium isolated from bark of Sonneratia apetala	31:99	nov., a novel endophytic bacterium isolated from bark of Sonneratia apetala.
31063121	2	9	attach	isolated	208:215	arg1	bark					241:244	surface-sterilized bark	222:244	surface-sterilized bark of Sonneratiaapetala sampled in Guangxi, China	222:291	A Gram-stain-positive, aerobic, rod-shaped, endospore-forming bacterium, designated strain M2MS4P-1T, was isolated from surface-sterilized bark of Sonneratiaapetala sampled in Guangxi, China.
31063121	2	9	attach	isolated	208:215	arg2	bacterium					164:172	A Gram-stain-positive, aerobic, rod-shaped, endospore-forming bacterium	102:172	A Gram-stain-positive, aerobic, rod-shaped, endospore-forming bacterium	102:172	A Gram-stain-positive, aerobic, rod-shaped, endospore-forming bacterium, designated strain M2MS4P-1T, was isolated from surface-sterilized bark of Sonneratiaapetala sampled in Guangxi, China.
31063121	3	10	theme	taxonomic	368:376	arg1	position					378:385	its taxonomic position	364:385	its taxonomic position	364:385	The bacterium was characterized by a polyphasic approach to determine its taxonomic position.
31063121	12	11	theme	chemotaxonomic	1394:1407	arg1	evidence					1409:1416	the phylogenetic, phenotypic and chemotaxonomic evidence	1361:1416	the phylogenetic, phenotypic and chemotaxonomic evidence	1361:1416	According to the phylogenetic, phenotypic and chemotaxonomic evidence, strain M2MS4P-1T was clearly distinguishable from other species with validly published names in the genus Cohnella and should therefore be classified as a novel species, for which we suggest the name Cohnellaendophytica sp.
31063121	5	12	theme	C.	668:669	arg1	HY-22RT					682:688	C. luojiensis HY-22RT	668:688	C. luojiensis HY-22RT	668:688	The average nucleotide identity value and estimated DDH value between strain M2MS4P-1T and the type strain of C. luojiensis HY-22RT were 79.2 and 20.1 %, respectively.
31063121	7	13	dep	optimum	885:891	arg1	7.0-8.0					897:903	pH 7.0-8.0	894:903	pH 7.0-8.0	894:903	Strain M2MS4P-1T grew in the pH range 6.0-9.0 (optimum, pH 7.0-8.0), at temperatures between 10-37 °C (30 °C) and in 0-1 % (w/v) NaCl (0 %).
31063121	12	14	theme	genus	1519:1523	arg1	Cohnella					1525:1532	the genus Cohnella	1515:1532	the genus Cohnella	1515:1532	According to the phylogenetic, phenotypic and chemotaxonomic evidence, strain M2MS4P-1T was clearly distinguishable from other species with validly published names in the genus Cohnella and should therefore be classified as a novel species, for which we suggest the name Cohnellaendophytica sp.
31063121	5	15	theme	strain	628:633	arg1	M2MS4P-1T					635:643	strain M2MS4P-1T	628:643	strain M2MS4P-1T	628:643	The average nucleotide identity value and estimated DDH value between strain M2MS4P-1T and the type strain of C. luojiensis HY-22RT were 79.2 and 20.1 %, respectively.
31063121	6	16	theme	diffusible	783:792	arg1	pigments					794:801	no diffusible pigments	780:801	no diffusible pigments	780:801	Neither substrate nor aerial mycelia were formed, and no diffusible pigments were observed on the media tested.
31063121	12	17	theme	phenotypic	1379:1388	arg1	evidence					1409:1416	the phylogenetic, phenotypic and chemotaxonomic evidence	1361:1416	the phylogenetic, phenotypic and chemotaxonomic evidence	1361:1416	According to the phylogenetic, phenotypic and chemotaxonomic evidence, strain M2MS4P-1T was clearly distinguishable from other species with validly published names in the genus Cohnella and should therefore be classified as a novel species, for which we suggest the name Cohnellaendophytica sp.
31063121	12	18	theme	Cohnellaendophytica	1619:1637	arg1	sp					1639:1640	the name Cohnellaendophytica sp	1610:1640	the name Cohnellaendophytica sp	1610:1640	According to the phylogenetic, phenotypic and chemotaxonomic evidence, strain M2MS4P-1T was clearly distinguishable from other species with validly published names in the genus Cohnella and should therefore be classified as a novel species, for which we suggest the name Cohnellaendophytica sp.
31063121	14	19	theme	43011T=CGMCC	1684:1695	arg1	M2MS4P-1T					1667:1675	M2MS4P-1T	1667:1675	M2MS4P-1T (=KCTC 43011T=CGMCC 1.13745T)	1667:1705	The type strain is M2MS4P-1T (=KCTC 43011T=CGMCC 1.13745T).
31063121	14	19	theme	43011T=CGMCC	1684:1695	arg1	1.13745T					1697:1704	=KCTC 43011T=CGMCC 1.13745T	1678:1704	=KCTC 43011T=CGMCC 1.13745T	1678:1704	The type strain is M2MS4P-1T (=KCTC 43011T=CGMCC 1.13745T).
31063121	5	20	theme	nucleotide	570:579	arg1	identity					581:588	The average nucleotide identity	558:588	The average nucleotide identity value	558:594	The average nucleotide identity value and estimated DDH value between strain M2MS4P-1T and the type strain of C. luojiensis HY-22RT were 79.2 and 20.1 %, respectively.
31063121	0	21	theme	endophytica	9:19	arg1	sp					21:22	Cohnella endophytica sp	0:22	Cohnella endophytica sp.	0:23	Cohnella endophytica sp.
31063121	5	22	theme	identity	581:588	arg1	value					590:594	The average nucleotide identity value	558:594	The average nucleotide identity value	558:594	The average nucleotide identity value and estimated DDH value between strain M2MS4P-1T and the type strain of C. luojiensis HY-22RT were 79.2 and 20.1 %, respectively.
31063121	7	23	theme	Strain	838:843	arg1	M2MS4P-1T					845:853	Strain M2MS4P-1T	838:853	Strain M2MS4P-1T	838:853	Strain M2MS4P-1T grew in the pH range 6.0-9.0 (optimum, pH 7.0-8.0), at temperatures between 10-37 °C (30 °C) and in 0-1 % (w/v) NaCl (0 %).
31063121	0	24	theme	Cohnella	0:7	arg1	sp					21:22	Cohnella endophytica sp	0:22	Cohnella endophytica sp.	0:23	Cohnella endophytica sp.
31063121	12	25	theme	other	1469:1473	arg1	species					1475:1481	other species	1469:1481	other species with validly published names in the genus Cohnella	1469:1532	According to the phylogenetic, phenotypic and chemotaxonomic evidence, strain M2MS4P-1T was clearly distinguishable from other species with validly published names in the genus Cohnella and should therefore be classified as a novel species, for which we suggest the name Cohnellaendophytica sp.
31063121	4	26	theme	rRNA	392:395	arg1	comparisons					411:421	16S rRNA gene sequence comparisons	388:421	16S rRNA gene sequence comparisons	388:421	16S rRNA gene sequence comparisons revealed that strain M2MS4P-1T belonged to the genus Cohnella and was most closely to Cohnella luojiensis HY-22RT (98.4 % similarity).
31063121	7	27	theme	pH	894:895	arg1	7.0-8.0					897:903	pH 7.0-8.0	894:903	pH 7.0-8.0	894:903	Strain M2MS4P-1T grew in the pH range 6.0-9.0 (optimum, pH 7.0-8.0), at temperatures between 10-37 °C (30 °C) and in 0-1 % (w/v) NaCl (0 %).
31063121	8	28	theme	predominant	983:993	arg1	menaquinone-7					1038:1050	menaquinone-7	1038:1050	menaquinone-7	1038:1050	The predominant isoprenoid quinone in strain M2MS4P-1T was menaquinone-7.
31063121	8	28	theme	predominant	983:993	arg1	quinone					1006:1012	The predominant isoprenoid quinone	979:1012	The predominant isoprenoid quinone in strain M2MS4P-1T	979:1032	The predominant isoprenoid quinone in strain M2MS4P-1T was menaquinone-7.
31063121	4	29	theme	16S	388:390	arg1	comparisons					411:421	16S rRNA gene sequence comparisons	388:421	16S rRNA gene sequence comparisons	388:421	16S rRNA gene sequence comparisons revealed that strain M2MS4P-1T belonged to the genus Cohnella and was most closely to Cohnella luojiensis HY-22RT (98.4 % similarity).
31063121	1	30	theme	Sonneratia	82:91	arg1	apetala					93:99	Sonneratia apetala	82:99	Sonneratia apetala	82:99	nov., a novel endophytic bacterium isolated from bark of Sonneratia apetala.
31063121	5	31	theme	estimated	600:608	arg1	value					614:618	estimated DDH value	600:618	estimated DDH value	600:618	The average nucleotide identity value and estimated DDH value between strain M2MS4P-1T and the type strain of C. luojiensis HY-22RT were 79.2 and 20.1 %, respectively.
31063121	10	32	theme	unidentified	1285:1296	arg1	phospholipids					1298:1310	two unidentified phospholipids	1281:1310	two unidentified phospholipids	1281:1310	The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, lysyl-phosphatidylglycerol, four unidentified aminophospholipids and two unidentified phospholipids.
31063121	14	33	theme	=KCTC	1678:1682	arg1	M2MS4P-1T					1667:1675	M2MS4P-1T	1667:1675	M2MS4P-1T (=KCTC 43011T=CGMCC 1.13745T)	1667:1705	The type strain is M2MS4P-1T (=KCTC 43011T=CGMCC 1.13745T).
31063121	14	33	theme	=KCTC	1678:1682	arg1	1.13745T					1697:1704	=KCTC 43011T=CGMCC 1.13745T	1678:1704	=KCTC 43011T=CGMCC 1.13745T	1678:1704	The type strain is M2MS4P-1T (=KCTC 43011T=CGMCC 1.13745T).
31063121	1	34	theme	apetala	93:99	arg1	bark					74:77	bark	74:77	bark of Sonneratia apetala	74:99	nov., a novel endophytic bacterium isolated from bark of Sonneratia apetala.
31063121	12	35	theme	name	1614:1617	arg1	sp					1639:1640	the name Cohnellaendophytica sp	1610:1640	the name Cohnellaendophytica sp	1610:1640	According to the phylogenetic, phenotypic and chemotaxonomic evidence, strain M2MS4P-1T was clearly distinguishable from other species with validly published names in the genus Cohnella and should therefore be classified as a novel species, for which we suggest the name Cohnellaendophytica sp.
31063121	12	36	from	species	1475:1481	arg1	distinguishable					1448:1462	distinguishable	1448:1462	distinguishable	1448:1462	According to the phylogenetic, phenotypic and chemotaxonomic evidence, strain M2MS4P-1T was clearly distinguishable from other species with validly published names in the genus Cohnella and should therefore be classified as a novel species, for which we suggest the name Cohnellaendophytica sp.
31063121	1	37	attach	isolated	60:67	arg1	bark					74:77	bark	74:77	bark of Sonneratia apetala	74:99	nov., a novel endophytic bacterium isolated from bark of Sonneratia apetala.
31063121	1	37	attach	isolated	60:67	arg2	bacterium					50:58	a novel endophytic bacterium	31:58	a novel endophytic bacterium isolated from bark of Sonneratia apetala	31:99	nov., a novel endophytic bacterium isolated from bark of Sonneratia apetala.
31063121	10	38	theme	unidentified	1245:1256	arg1	aminophospholipids					1258:1275	four unidentified aminophospholipids	1240:1275	four unidentified aminophospholipids	1240:1275	The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, lysyl-phosphatidylglycerol, four unidentified aminophospholipids and two unidentified phospholipids.
31063121	9	39	theme	major	1057:1061	arg1	acids					1069:1073	The major fatty acids	1053:1073	The major fatty acids	1053:1073	The major fatty acids were anteiso-C15 : 0 and iso-C16 : 0.
31063121	9	39	theme	major	1057:1061	arg1	anteiso-C15 					1080:1091	anteiso-C15 	1080:1091	anteiso-C15 	1080:1091	The major fatty acids were anteiso-C15 : 0 and iso-C16 : 0.
31063121	11	40	theme	51.5 mol	1337:1344	arg1	G+C content					1321:1331	The DNA G+C content	1313:1331	The DNA G+C content	1313:1331	The DNA G+C content was 51.5 mol%.
31063121	11	40	theme	51.5 mol	1337:1344	arg1	%					1345:1345	51.5 mol%	1337:1345	51.5 mol%	1337:1345	The DNA G+C content was 51.5 mol%.
31063121	4	41	theme	98.4 	538:542	arg1	%					543:543	%	543:543	%	543:543	16S rRNA gene sequence comparisons revealed that strain M2MS4P-1T belonged to the genus Cohnella and was most closely to Cohnella luojiensis HY-22RT (98.4 % similarity).
31063121	8	42	theme	isoprenoid	995:1004	arg1	menaquinone-7					1038:1050	menaquinone-7	1038:1050	menaquinone-7	1038:1050	The predominant isoprenoid quinone in strain M2MS4P-1T was menaquinone-7.
31063121	8	42	theme	isoprenoid	995:1004	arg1	quinone					1006:1012	The predominant isoprenoid quinone	979:1012	The predominant isoprenoid quinone in strain M2MS4P-1T	979:1032	The predominant isoprenoid quinone in strain M2MS4P-1T was menaquinone-7.
31063121	2	43	theme	surface-sterilized	222:239	arg1	bark					241:244	surface-sterilized bark	222:244	surface-sterilized bark of Sonneratiaapetala sampled in Guangxi, China	222:291	A Gram-stain-positive, aerobic, rod-shaped, endospore-forming bacterium, designated strain M2MS4P-1T, was isolated from surface-sterilized bark of Sonneratiaapetala sampled in Guangxi, China.
31063121	7	44	theme	%	959:959	arg1	NaCl					967:970	0-1 % (w/v) NaCl	955:970	0-1 % (w/v) NaCl (0 %)	955:976	Strain M2MS4P-1T grew in the pH range 6.0-9.0 (optimum, pH 7.0-8.0), at temperatures between 10-37 °C (30 °C) and in 0-1 % (w/v) NaCl (0 %).
31063121	7	44	theme	%	959:959	arg1	%					975:975	0 %	973:975	0 %	973:975	Strain M2MS4P-1T grew in the pH range 6.0-9.0 (optimum, pH 7.0-8.0), at temperatures between 10-37 °C (30 °C) and in 0-1 % (w/v) NaCl (0 %).
31063121	4	45	theme	%	543:543	arg1	similarity					545:554	98.4 % similarity	538:554	98.4 % similarity	538:554	16S rRNA gene sequence comparisons revealed that strain M2MS4P-1T belonged to the genus Cohnella and was most closely to Cohnella luojiensis HY-22RT (98.4 % similarity).
31063121	11	46	theme	DNA	1317:1319	arg1	G+C content					1321:1331	The DNA G+C content	1313:1331	The DNA G+C content	1313:1331	The DNA G+C content was 51.5 mol%.
31063121	11	46	theme	DNA	1317:1319	arg1	%					1345:1345	51.5 mol%	1337:1345	51.5 mol%	1337:1345	The DNA G+C content was 51.5 mol%.
31063121	4	47	dep	HY-22RT	529:535	arg1	similarity					545:554	98.4 % similarity	538:554	98.4 % similarity	538:554	16S rRNA gene sequence comparisons revealed that strain M2MS4P-1T belonged to the genus Cohnella and was most closely to Cohnella luojiensis HY-22RT (98.4 % similarity).
31063121	5	48	theme	luojiensis	671:680	arg1	HY-22RT					682:688	C. luojiensis HY-22RT	668:688	C. luojiensis HY-22RT	668:688	The average nucleotide identity value and estimated DDH value between strain M2MS4P-1T and the type strain of C. luojiensis HY-22RT were 79.2 and 20.1 %, respectively.
31063121	4	49	theme	genus	470:474	arg1	Cohnella					476:483	the genus Cohnella	466:483	the genus Cohnella	466:483	16S rRNA gene sequence comparisons revealed that strain M2MS4P-1T belonged to the genus Cohnella and was most closely to Cohnella luojiensis HY-22RT (98.4 % similarity).
31063121	12	50	theme	novel	1574:1578	arg1	M2MS4P-1T					1426:1434	strain M2MS4P-1T	1419:1434	strain M2MS4P-1T	1419:1434	According to the phylogenetic, phenotypic and chemotaxonomic evidence, strain M2MS4P-1T was clearly distinguishable from other species with validly published names in the genus Cohnella and should therefore be classified as a novel species, for which we suggest the name Cohnellaendophytica sp.
31063121	12	50	theme	novel	1574:1578	arg1	species					1580:1586	a novel species	1572:1586	a novel species	1572:1586	According to the phylogenetic, phenotypic and chemotaxonomic evidence, strain M2MS4P-1T was clearly distinguishable from other species with validly published names in the genus Cohnella and should therefore be classified as a novel species, for which we suggest the name Cohnellaendophytica sp.
31063121	5	51	theme	HY-22RT	682:688	arg1	strain					658:663	the type strain	649:663	the type strain of C. luojiensis HY-22RT	649:688	The average nucleotide identity value and estimated DDH value between strain M2MS4P-1T and the type strain of C. luojiensis HY-22RT were 79.2 and 20.1 %, respectively.
31063121	5	51	theme	HY-22RT	682:688	arg1	M2MS4P-1T					635:643	strain M2MS4P-1T	628:643	strain M2MS4P-1T	628:643	The average nucleotide identity value and estimated DDH value between strain M2MS4P-1T and the type strain of C. luojiensis HY-22RT were 79.2 and 20.1 %, respectively.
31063121	4	52	theme	strain	437:442	arg1	M2MS4P-1T					444:452	strain M2MS4P-1T	437:452	strain M2MS4P-1T	437:452	16S rRNA gene sequence comparisons revealed that strain M2MS4P-1T belonged to the genus Cohnella and was most closely to Cohnella luojiensis HY-22RT (98.4 % similarity).
31063121	6	53	theme	aerial	748:753	arg1	mycelia					755:761	aerial mycelia	748:761	aerial mycelia	748:761	Neither substrate nor aerial mycelia were formed, and no diffusible pigments were observed on the media tested.
31063121	2	54	dep	Gram-stain-positive	104:122	arg1	endospore-forming					146:162	endospore-forming	146:162	endospore-forming	146:162	A Gram-stain-positive, aerobic, rod-shaped, endospore-forming bacterium, designated strain M2MS4P-1T, was isolated from surface-sterilized bark of Sonneratiaapetala sampled in Guangxi, China.
31063121	2	54	dep	Gram-stain-positive	104:122	arg1	rod-shaped					134:143	rod-shaped	134:143	rod-shaped	134:143	A Gram-stain-positive, aerobic, rod-shaped, endospore-forming bacterium, designated strain M2MS4P-1T, was isolated from surface-sterilized bark of Sonneratiaapetala sampled in Guangxi, China.
31063121	2	54	dep	Gram-stain-positive	104:122	arg1	aerobic					125:131	aerobic	125:131	aerobic	125:131	A Gram-stain-positive, aerobic, rod-shaped, endospore-forming bacterium, designated strain M2MS4P-1T, was isolated from surface-sterilized bark of Sonneratiaapetala sampled in Guangxi, China.
31063121	7	55	theme	w/v	962:964	arg1	NaCl					967:970	0-1 % (w/v) NaCl	955:970	0-1 % (w/v) NaCl (0 %)	955:976	Strain M2MS4P-1T grew in the pH range 6.0-9.0 (optimum, pH 7.0-8.0), at temperatures between 10-37 °C (30 °C) and in 0-1 % (w/v) NaCl (0 %).
31063121	7	55	theme	w/v	962:964	arg1	%					975:975	0 %	973:975	0 %	973:975	Strain M2MS4P-1T grew in the pH range 6.0-9.0 (optimum, pH 7.0-8.0), at temperatures between 10-37 °C (30 °C) and in 0-1 % (w/v) NaCl (0 %).
31063121	14	56	theme	type	1652:1655	arg1	M2MS4P-1T					1667:1675	M2MS4P-1T	1667:1675	M2MS4P-1T (=KCTC 43011T=CGMCC 1.13745T)	1667:1705	The type strain is M2MS4P-1T (=KCTC 43011T=CGMCC 1.13745T).
31063121	14	56	theme	type	1652:1655	arg1	strain					1657:1662	The type strain	1648:1662	The type strain	1648:1662	The type strain is M2MS4P-1T (=KCTC 43011T=CGMCC 1.13745T).
31063121	5	57	theme	DDH	610:612	arg1	value					614:618	estimated DDH value	600:618	estimated DDH value	600:618	The average nucleotide identity value and estimated DDH value between strain M2MS4P-1T and the type strain of C. luojiensis HY-22RT were 79.2 and 20.1 %, respectively.
31063121	9	58	dep	anteiso-C15 	1080:1091	arg1	 0					1109:1110	 0	1109:1110	anteiso-C15 : 0 and iso-C16 : 0	1080:1110	The major fatty acids were anteiso-C15 : 0 and iso-C16 : 0.
31063121	9	58	dep	anteiso-C15 	1080:1091	arg1	iso-C16 					1100:1107	iso-C16 	1100:1107	iso-C16 	1100:1107	The major fatty acids were anteiso-C15 : 0 and iso-C16 : 0.
31063121	9	58	dep	anteiso-C15 	1080:1091	arg1	 0					1093:1094	 0	1093:1094	 0	1093:1094	The major fatty acids were anteiso-C15 : 0 and iso-C16 : 0.
31063121	8	59	from	quinone	1006:1012	arg1	M2MS4P-1T					1024:1032	strain M2MS4P-1T	1017:1032	strain M2MS4P-1T	1017:1032	The predominant isoprenoid quinone in strain M2MS4P-1T was menaquinone-7.
31063121	5	60	theme	average	562:568	arg1	identity					581:588	The average nucleotide identity	558:588	The average nucleotide identity value	558:594	The average nucleotide identity value and estimated DDH value between strain M2MS4P-1T and the type strain of C. luojiensis HY-22RT were 79.2 and 20.1 %, respectively.
31063121	12	61	theme	phylogenetic	1365:1376	arg1	evidence					1409:1416	the phylogenetic, phenotypic and chemotaxonomic evidence	1361:1416	the phylogenetic, phenotypic and chemotaxonomic evidence	1361:1416	According to the phylogenetic, phenotypic and chemotaxonomic evidence, strain M2MS4P-1T was clearly distinguishable from other species with validly published names in the genus Cohnella and should therefore be classified as a novel species, for which we suggest the name Cohnellaendophytica sp.
31063121	9	62	theme	fatty	1063:1067	arg1	acids					1069:1073	The major fatty acids	1053:1073	The major fatty acids	1053:1073	The major fatty acids were anteiso-C15 : 0 and iso-C16 : 0.
31063121	9	62	theme	fatty	1063:1067	arg1	anteiso-C15 					1080:1091	anteiso-C15 	1080:1091	anteiso-C15 	1080:1091	The major fatty acids were anteiso-C15 : 0 and iso-C16 : 0.
31063121	1	63	dep	bacterium	50:58	arg1	nov.					25:28	nov.	25:28	nov.	25:28	nov., a novel endophytic bacterium isolated from bark of Sonneratia apetala.
31063121	3	64	theme	polyphasic	331:340	arg1	approach					342:349	a polyphasic approach	329:349	a polyphasic approach to determine its taxonomic position	329:385	The bacterium was characterized by a polyphasic approach to determine its taxonomic position.
31063121	2	65	theme	strain	186:191	arg1	M2MS4P-1T					193:201	strain M2MS4P-1T	186:201	strain M2MS4P-1T	186:201	A Gram-stain-positive, aerobic, rod-shaped, endospore-forming bacterium, designated strain M2MS4P-1T, was isolated from surface-sterilized bark of Sonneratiaapetala sampled in Guangxi, China.
31063121	2	66	theme	Sonneratiaapetala	249:265	arg1	bark					241:244	surface-sterilized bark	222:244	surface-sterilized bark of Sonneratiaapetala sampled in Guangxi, China	222:291	A Gram-stain-positive, aerobic, rod-shaped, endospore-forming bacterium, designated strain M2MS4P-1T, was isolated from surface-sterilized bark of Sonneratiaapetala sampled in Guangxi, China.
31063121	10	67	theme	polar	1117:1121	arg1	lipids					1123:1128	The polar lipids	1113:1128	The polar lipids	1113:1128	The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, lysyl-phosphatidylglycerol, four unidentified aminophospholipids and two unidentified phospholipids.
31063121	4	68	theme	Cohnella	509:516	arg1	luojiensis					518:527	Cohnella luojiensis	509:527	Cohnella luojiensis	509:527	16S rRNA gene sequence comparisons revealed that strain M2MS4P-1T belonged to the genus Cohnella and was most closely to Cohnella luojiensis HY-22RT (98.4 % similarity).
31063121	1	69	theme	novel	33:37	arg1	bacterium					50:58	a novel endophytic bacterium	31:58	a novel endophytic bacterium isolated from bark of Sonneratia apetala	31:99	nov., a novel endophytic bacterium isolated from bark of Sonneratia apetala.
31063121	12	70	from	names	1506:1510	arg1	Cohnella					1525:1532	the genus Cohnella	1515:1532	the genus Cohnella	1515:1532	According to the phylogenetic, phenotypic and chemotaxonomic evidence, strain M2MS4P-1T was clearly distinguishable from other species with validly published names in the genus Cohnella and should therefore be classified as a novel species, for which we suggest the name Cohnellaendophytica sp.
31063121	7	71	theme	pH	867:868	arg1	range					870:874	the pH range 6.0-9.0	863:882	the pH range 6.0-9.0 (optimum, pH 7.0-8.0)	863:904	Strain M2MS4P-1T grew in the pH range 6.0-9.0 (optimum, pH 7.0-8.0), at temperatures between 10-37 °C (30 °C) and in 0-1 % (w/v) NaCl (0 %).
31063121	7	71	theme	pH	867:868	arg1	optimum					885:891	optimum	885:891	optimum	885:891	Strain M2MS4P-1T grew in the pH range 6.0-9.0 (optimum, pH 7.0-8.0), at temperatures between 10-37 °C (30 °C) and in 0-1 % (w/v) NaCl (0 %).
31063121	6	72	located	observed	808:815	arg1	media					824:828	the media	820:828	the media tested	820:835	Neither substrate nor aerial mycelia were formed, and no diffusible pigments were observed on the media tested.
31063121	6	72	located	observed	808:815	arg2	pigments					794:801	no diffusible pigments	780:801	no diffusible pigments	780:801	Neither substrate nor aerial mycelia were formed, and no diffusible pigments were observed on the media tested.
30641899	3	0	theme	study	307:311	arg1	aim					295:297	The aim	291:297	The aim of this study	291:311	The aim of this study was to investigate the physicochemical characteristics, pharmacokinetics behavior, and cytotoxic efficacy of docetaxel (DTX)-loaded CS-coated FLs (C-FLs).
30641899	4	1	dep	evaporation	521:531	arg1	methods					562:568	methods	562:568	methods	562:568	DTX-loaded FLs and C-FLs were produced via thin-film evaporation and electrostatic deposition methods, respectively.
30641899	3	2	dep	pharmacokinetics	369:384	arg1	behavior					386:393	behavior	386:393	behavior	386:393	The aim of this study was to investigate the physicochemical characteristics, pharmacokinetics behavior, and cytotoxic efficacy of docetaxel (DTX)-loaded CS-coated FLs (C-FLs).
30641899	7	3	theme	particle	944:951	arg1	sizes					953:957	particle sizes	944:957	particle sizes in the nano-range, 137.6 ± 6.3 to 238.2 ± 14.2 nm, and an EE% of 59⁻94%	944:1029	Various FLs, dependent on the type of surfactant, were formed with particle sizes in the nano-range, 137.6 ± 6.3 to 238.2 ± 14.2 nm, and an EE% of 59⁻94%.
30641899	0	4	theme	Docetaxel	90:98	arg1	Bioavailability					71:85	Bioavailability	71:85	Bioavailability	71:85	Chitosan-Coated Flexible Liposomes Magnify the Anticancer Activity and Bioavailability of Docetaxel: Impact on Composition.
30641899	0	4	theme	Docetaxel	90:98	arg1	Activity					58:65	Anticancer Activity	47:65	Anticancer Activity	47:65	Chitosan-Coated Flexible Liposomes Magnify the Anticancer Activity and Bioavailability of Docetaxel: Impact on Composition.
30641899	5	5	theme	potential	660:668	arg1	profiles					731:738	the particle size, zeta potential, encapsulation efficiency (EE%), morphology, and DTX release profiles	636:738	the particle size, zeta potential, encapsulation efficiency (EE%), morphology, and DTX release profiles	636:738	To explore their physicochemical characterization, the particle size, zeta potential, encapsulation efficiency (EE%), morphology, and DTX release profiles were determined.
30641899	8	6	theme	C-FL	1199:1202	arg1	evident					1233:1239	evident	1233:1239	evident	1233:1239	Moreover, the zeta potential shifted from a negative to a positive value for C-FL with increased particle size and EE%, and the in vitro sustained-release profiles of C-FL compared to those of FL were evident.
30641899	8	6	theme	C-FL	1199:1202	arg1	profiles					1187:1194	the in vitro sustained-release profiles	1156:1194	the in vitro sustained-release profiles of C-FL compared to those of FL	1156:1226	Moreover, the zeta potential shifted from a negative to a positive value for C-FL with increased particle size and EE%, and the in vitro sustained-release profiles of C-FL compared to those of FL were evident.
30641899	9	7	theme	pharmacokinetic	1343:1357	arg1	parameters					1359:1368	enhanced pharmacokinetic parameters	1334:1368	enhanced pharmacokinetic parameters	1334:1368	The optimized C-FL containing sodium deoxycholate (NDC) and dicetyl phosphate (DP) elicited enhanced pharmacokinetic parameters and cytotoxic efficiency compared to those of the uncoated ones and Onkotaxel®.
30641899	5	8	theme	zeta	655:658	arg1	potential					660:668	zeta potential	655:668	zeta potential	655:668	To explore their physicochemical characterization, the particle size, zeta potential, encapsulation efficiency (EE%), morphology, and DTX release profiles were determined.
30641899	7	9	dep	±	984:984	arg1	nm					1006:1007	± 14.2 nm	999:1007	137.6 ± 6.3 to 238.2 ± 14.2 nm	978:1007	Various FLs, dependent on the type of surfactant, were formed with particle sizes in the nano-range, 137.6 ± 6.3 to 238.2 ± 14.2 nm, and an EE% of 59⁻94%.
30641899	5	10	theme	physicochemical	602:616	arg1	characterization					618:633	their physicochemical characterization	596:633	their physicochemical characterization	596:633	To explore their physicochemical characterization, the particle size, zeta potential, encapsulation efficiency (EE%), morphology, and DTX release profiles were determined.
30641899	5	11	theme	DTX	719:721	arg1	profiles					731:738	the particle size, zeta potential, encapsulation efficiency (EE%), morphology, and DTX release profiles	636:738	the particle size, zeta potential, encapsulation efficiency (EE%), morphology, and DTX release profiles	636:738	To explore their physicochemical characterization, the particle size, zeta potential, encapsulation efficiency (EE%), morphology, and DTX release profiles were determined.
30641899	5	12	theme	encapsulation	671:683	arg1	efficiency					685:694	encapsulation efficiency	671:694	encapsulation efficiency (EE%)	671:700	To explore their physicochemical characterization, the particle size, zeta potential, encapsulation efficiency (EE%), morphology, and DTX release profiles were determined.
30641899	5	12	theme	encapsulation	671:683	arg1	%					699:699	EE%	697:699	EE%	697:699	To explore their physicochemical characterization, the particle size, zeta potential, encapsulation efficiency (EE%), morphology, and DTX release profiles were determined.
30641899	7	13	theme	surfactant	915:924	arg1	type					907:910	the type	903:910	the type of surfactant	903:924	Various FLs, dependent on the type of surfactant, were formed with particle sizes in the nano-range, 137.6 ± 6.3 to 238.2 ± 14.2 nm, and an EE% of 59⁻94%.
30641899	5	14	theme	release	723:729	arg1	profiles					731:738	the particle size, zeta potential, encapsulation efficiency (EE%), morphology, and DTX release profiles	636:738	the particle size, zeta potential, encapsulation efficiency (EE%), morphology, and DTX release profiles	636:738	To explore their physicochemical characterization, the particle size, zeta potential, encapsulation efficiency (EE%), morphology, and DTX release profiles were determined.
30641899	8	15	theme	in	1160:1161	arg1	evident					1233:1239	evident	1233:1239	evident	1233:1239	Moreover, the zeta potential shifted from a negative to a positive value for C-FL with increased particle size and EE%, and the in vitro sustained-release profiles of C-FL compared to those of FL were evident.
30641899	8	15	theme	in	1160:1161	arg1	profiles					1187:1194	the in vitro sustained-release profiles	1156:1194	the in vitro sustained-release profiles of C-FL compared to those of FL	1156:1226	Moreover, the zeta potential shifted from a negative to a positive value for C-FL with increased particle size and EE%, and the in vitro sustained-release profiles of C-FL compared to those of FL were evident.
30641899	7	16	theme	EE	1017:1018	arg1	%					1019:1019	an EE%	1014:1019	an EE% of 59⁻94%	1014:1029	Various FLs, dependent on the type of surfactant, were formed with particle sizes in the nano-range, 137.6 ± 6.3 to 238.2 ± 14.2 nm, and an EE% of 59⁻94%.
30641899	7	16	theme	EE	1017:1018	arg1	%					1029:1029	59⁻94%	1024:1029	59⁻94%	1024:1029	Various FLs, dependent on the type of surfactant, were formed with particle sizes in the nano-range, 137.6 ± 6.3 to 238.2 ± 14.2 nm, and an EE% of 59⁻94%.
30641899	8	17	theme	EE	1147:1148	arg1	%					1149:1149	EE%	1147:1149	EE%	1147:1149	Moreover, the zeta potential shifted from a negative to a positive value for C-FL with increased particle size and EE%, and the in vitro sustained-release profiles of C-FL compared to those of FL were evident.
30641899	2	18	theme	delivery	270:277	arg1	properties					279:288	their drug delivery properties	259:288	their drug delivery properties	259:288	Coating them with chitosan (CS) could improve their drug delivery properties.
30641899	10	19	theme	DTX	1511:1513	arg1	delivery					1515:1522	DTX delivery	1511:1522	DTX delivery	1511:1522	In conclusion, this approach offers a promising solution for DTX delivery.
30641899	1	20	theme	promising	167:175	arg1	nano-carriers					177:189	promising nano-carriers	167:189	promising nano-carriers for anticancer drugs	167:210	Flexible liposomes (FLs) were developed as promising nano-carriers for anticancer drugs.
30641899	1	20	theme	promising	167:175	arg1	liposomes					133:141	Flexible liposomes	124:141	Flexible liposomes (FLs)	124:147	Flexible liposomes (FLs) were developed as promising nano-carriers for anticancer drugs.
30641899	3	21	theme	physicochemical	336:350	arg1	efficacy					410:417	cytotoxic efficacy	400:417	cytotoxic efficacy	400:417	The aim of this study was to investigate the physicochemical characteristics, pharmacokinetics behavior, and cytotoxic efficacy of docetaxel (DTX)-loaded CS-coated FLs (C-FLs).
30641899	3	21	theme	physicochemical	336:350	arg1	pharmacokinetics					369:384	pharmacokinetics	369:384	pharmacokinetics	369:384	The aim of this study was to investigate the physicochemical characteristics, pharmacokinetics behavior, and cytotoxic efficacy of docetaxel (DTX)-loaded CS-coated FLs (C-FLs).
30641899	3	21	theme	physicochemical	336:350	arg1	characteristics					352:366	the physicochemical characteristics	332:366	the physicochemical characteristics	332:366	The aim of this study was to investigate the physicochemical characteristics, pharmacokinetics behavior, and cytotoxic efficacy of docetaxel (DTX)-loaded CS-coated FLs (C-FLs).
30641899	5	22	theme	particle	640:647	arg1	size					649:652	particle size	640:652	particle size	640:652	To explore their physicochemical characterization, the particle size, zeta potential, encapsulation efficiency (EE%), morphology, and DTX release profiles were determined.
30641899	2	23	theme	drug	265:268	arg1	properties					279:288	their drug delivery properties	259:288	their drug delivery properties	259:288	Coating them with chitosan (CS) could improve their drug delivery properties.
30641899	3	24	theme	-loaded	437:443	arg1	C-FLs					460:464	docetaxel (DTX)-loaded CS-coated FLs (C-FLs)	422:465	docetaxel (DTX)-loaded CS-coated FLs (C-FLs)	422:465	The aim of this study was to investigate the physicochemical characteristics, pharmacokinetics behavior, and cytotoxic efficacy of docetaxel (DTX)-loaded CS-coated FLs (C-FLs).
30641899	0	25	theme	Flexible	16:23	arg1	Liposomes					25:33	Chitosan-Coated Flexible Liposomes	0:33	Chitosan-Coated Flexible Liposomes	0:33	Chitosan-Coated Flexible Liposomes Magnify the Anticancer Activity and Bioavailability of Docetaxel: Impact on Composition.
30641899	4	26	theme	DTX-loaded	468:477	arg1	FLs					479:481	DTX-loaded FLs	468:481	DTX-loaded FLs	468:481	DTX-loaded FLs and C-FLs were produced via thin-film evaporation and electrostatic deposition methods, respectively.
30641899	5	27	theme	size	649:652	arg1	profiles					731:738	the particle size, zeta potential, encapsulation efficiency (EE%), morphology, and DTX release profiles	636:738	the particle size, zeta potential, encapsulation efficiency (EE%), morphology, and DTX release profiles	636:738	To explore their physicochemical characterization, the particle size, zeta potential, encapsulation efficiency (EE%), morphology, and DTX release profiles were determined.
30641899	6	28	theme	pharmacokinetic	770:784	arg1	studies					786:792	pharmacokinetic studies	770:792	pharmacokinetic studies	770:792	In addition, pharmacokinetic studies were performed, and cytotoxic effect was assessed using colon cancer cells (HT29).
30641899	7	29	from	sizes	953:957	arg1	nano-range					966:975	the nano-range	962:975	the nano-range	962:975	Various FLs, dependent on the type of surfactant, were formed with particle sizes in the nano-range, 137.6 ± 6.3 to 238.2 ± 14.2 nm, and an EE% of 59⁻94%.
30641899	7	29	from	sizes	953:957	arg1	±					984:984	137.6 ± 6.3 to 238.2	978:997	137.6 ± 6.3 to 238.2 ± 14.2 nm	978:1007	Various FLs, dependent on the type of surfactant, were formed with particle sizes in the nano-range, 137.6 ± 6.3 to 238.2 ± 14.2 nm, and an EE% of 59⁻94%.
30641899	7	29	from	sizes	953:957	arg1	%					1019:1019	an EE%	1014:1019	an EE% of 59⁻94%	1014:1029	Various FLs, dependent on the type of surfactant, were formed with particle sizes in the nano-range, 137.6 ± 6.3 to 238.2 ± 14.2 nm, and an EE% of 59⁻94%.
30641899	7	29	from	sizes	953:957	arg1	%					1029:1029	59⁻94%	1024:1029	59⁻94%	1024:1029	Various FLs, dependent on the type of surfactant, were formed with particle sizes in the nano-range, 137.6 ± 6.3 to 238.2 ± 14.2 nm, and an EE% of 59⁻94%.
30641899	0	30	theme	Chitosan-Coated	0:14	arg1	Liposomes					25:33	Chitosan-Coated Flexible Liposomes	0:33	Chitosan-Coated Flexible Liposomes	0:33	Chitosan-Coated Flexible Liposomes Magnify the Anticancer Activity and Bioavailability of Docetaxel: Impact on Composition.
30641899	6	31	theme	cytotoxic	814:822	arg1	effect					824:829	cytotoxic effect	814:829	cytotoxic effect	814:829	In addition, pharmacokinetic studies were performed, and cytotoxic effect was assessed using colon cancer cells (HT29).
30641899	8	32	theme	zeta	1046:1049	arg1	potential					1051:1059	the zeta potential	1042:1059	the zeta potential	1042:1059	Moreover, the zeta potential shifted from a negative to a positive value for C-FL with increased particle size and EE%, and the in vitro sustained-release profiles of C-FL compared to those of FL were evident.
30641899	1	33	theme	anticancer	195:204	arg1	drugs					206:210	anticancer drugs	195:210	anticancer drugs	195:210	Flexible liposomes (FLs) were developed as promising nano-carriers for anticancer drugs.
30641899	8	34	theme	positive	1090:1097	arg1	value					1099:1103	a positive value	1088:1103	a positive value for C-FL with increased particle size and EE%	1088:1149	Moreover, the zeta potential shifted from a negative to a positive value for C-FL with increased particle size and EE%, and the in vitro sustained-release profiles of C-FL compared to those of FL were evident.
30641899	4	35	theme	electrostatic	537:549	arg1	deposition					551:560	electrostatic deposition	537:560	electrostatic deposition	537:560	DTX-loaded FLs and C-FLs were produced via thin-film evaporation and electrostatic deposition methods, respectively.
30641899	7	36	theme	%	1029:1029	arg1	nano-range					966:975	the nano-range	962:975	the nano-range	962:975	Various FLs, dependent on the type of surfactant, were formed with particle sizes in the nano-range, 137.6 ± 6.3 to 238.2 ± 14.2 nm, and an EE% of 59⁻94%.
30641899	7	36	theme	%	1029:1029	arg1	±					984:984	137.6 ± 6.3 to 238.2	978:997	137.6 ± 6.3 to 238.2 ± 14.2 nm	978:1007	Various FLs, dependent on the type of surfactant, were formed with particle sizes in the nano-range, 137.6 ± 6.3 to 238.2 ± 14.2 nm, and an EE% of 59⁻94%.
30641899	7	36	theme	%	1029:1029	arg1	%					1019:1019	an EE%	1014:1019	an EE% of 59⁻94%	1014:1029	Various FLs, dependent on the type of surfactant, were formed with particle sizes in the nano-range, 137.6 ± 6.3 to 238.2 ± 14.2 nm, and an EE% of 59⁻94%.
30641899	7	36	theme	%	1029:1029	arg1	%					1029:1029	59⁻94%	1024:1029	59⁻94%	1024:1029	Various FLs, dependent on the type of surfactant, were formed with particle sizes in the nano-range, 137.6 ± 6.3 to 238.2 ± 14.2 nm, and an EE% of 59⁻94%.
30641899	7	37	theme	±	999:999	arg1	nm					1006:1007	± 14.2 nm	999:1007	137.6 ± 6.3 to 238.2 ± 14.2 nm	978:1007	Various FLs, dependent on the type of surfactant, were formed with particle sizes in the nano-range, 137.6 ± 6.3 to 238.2 ± 14.2 nm, and an EE% of 59⁻94%.
30641899	9	38	theme	uncoated	1420:1427	arg1	ones					1429:1432	the uncoated ones	1416:1432	the uncoated ones	1416:1432	The optimized C-FL containing sodium deoxycholate (NDC) and dicetyl phosphate (DP) elicited enhanced pharmacokinetic parameters and cytotoxic efficiency compared to those of the uncoated ones and Onkotaxel®.
30641899	3	39	theme	C-FLs	460:464	arg1	efficacy					410:417	cytotoxic efficacy	400:417	cytotoxic efficacy	400:417	The aim of this study was to investigate the physicochemical characteristics, pharmacokinetics behavior, and cytotoxic efficacy of docetaxel (DTX)-loaded CS-coated FLs (C-FLs).
30641899	3	39	theme	C-FLs	460:464	arg1	pharmacokinetics					369:384	pharmacokinetics	369:384	pharmacokinetics	369:384	The aim of this study was to investigate the physicochemical characteristics, pharmacokinetics behavior, and cytotoxic efficacy of docetaxel (DTX)-loaded CS-coated FLs (C-FLs).
30641899	3	39	theme	C-FLs	460:464	arg1	characteristics					352:366	the physicochemical characteristics	332:366	the physicochemical characteristics	332:366	The aim of this study was to investigate the physicochemical characteristics, pharmacokinetics behavior, and cytotoxic efficacy of docetaxel (DTX)-loaded CS-coated FLs (C-FLs).
30641899	0	40	theme	Anticancer	47:56	arg1	Activity					58:65	Anticancer Activity	47:65	Anticancer Activity	47:65	Chitosan-Coated Flexible Liposomes Magnify the Anticancer Activity and Bioavailability of Docetaxel: Impact on Composition.
30641899	5	41	theme	morphology	703:712	arg1	profiles					731:738	the particle size, zeta potential, encapsulation efficiency (EE%), morphology, and DTX release profiles	636:738	the particle size, zeta potential, encapsulation efficiency (EE%), morphology, and DTX release profiles	636:738	To explore their physicochemical characterization, the particle size, zeta potential, encapsulation efficiency (EE%), morphology, and DTX release profiles were determined.
30641899	6	42	theme	cancer	856:861	arg1	HT29					870:873	HT29	870:873	HT29	870:873	In addition, pharmacokinetic studies were performed, and cytotoxic effect was assessed using colon cancer cells (HT29).
30641899	6	42	theme	cancer	856:861	arg1	cells					863:867	colon cancer cells	850:867	colon cancer cells (HT29)	850:874	In addition, pharmacokinetic studies were performed, and cytotoxic effect was assessed using colon cancer cells (HT29).
30641899	3	43	theme	CS-coated	445:453	arg1	C-FLs					460:464	docetaxel (DTX)-loaded CS-coated FLs (C-FLs)	422:465	docetaxel (DTX)-loaded CS-coated FLs (C-FLs)	422:465	The aim of this study was to investigate the physicochemical characteristics, pharmacokinetics behavior, and cytotoxic efficacy of docetaxel (DTX)-loaded CS-coated FLs (C-FLs).
30641899	8	44	theme	particle	1129:1136	arg1	size					1138:1141	increased particle size	1119:1141	increased particle size	1119:1141	Moreover, the zeta potential shifted from a negative to a positive value for C-FL with increased particle size and EE%, and the in vitro sustained-release profiles of C-FL compared to those of FL were evident.
30641899	4	45	theme	thin-film	511:519	arg1	evaporation					521:531	thin-film evaporation	511:531	thin-film evaporation	511:531	DTX-loaded FLs and C-FLs were produced via thin-film evaporation and electrostatic deposition methods, respectively.
30641899	10	46	theme	promising	1488:1496	arg1	solution					1498:1505	a promising solution	1486:1505	a promising solution for DTX delivery	1486:1522	In conclusion, this approach offers a promising solution for DTX delivery.
30641899	6	47	theme	colon	850:854	arg1	HT29					870:873	HT29	870:873	HT29	870:873	In addition, pharmacokinetic studies were performed, and cytotoxic effect was assessed using colon cancer cells (HT29).
30641899	6	47	theme	colon	850:854	arg1	cells					863:867	colon cancer cells	850:867	colon cancer cells (HT29)	850:874	In addition, pharmacokinetic studies were performed, and cytotoxic effect was assessed using colon cancer cells (HT29).
30641899	0	48	dep	Activity	58:65	arg1	the					43:45	the	43:45	the	43:45	Chitosan-Coated Flexible Liposomes Magnify the Anticancer Activity and Bioavailability of Docetaxel: Impact on Composition.
30641899	3	49	theme	FLs	455:457	arg1	C-FLs					460:464	docetaxel (DTX)-loaded CS-coated FLs (C-FLs)	422:465	docetaxel (DTX)-loaded CS-coated FLs (C-FLs)	422:465	The aim of this study was to investigate the physicochemical characteristics, pharmacokinetics behavior, and cytotoxic efficacy of docetaxel (DTX)-loaded CS-coated FLs (C-FLs).
30641899	3	50	theme	cytotoxic	400:408	arg1	efficacy					410:417	cytotoxic efficacy	400:417	cytotoxic efficacy	400:417	The aim of this study was to investigate the physicochemical characteristics, pharmacokinetics behavior, and cytotoxic efficacy of docetaxel (DTX)-loaded CS-coated FLs (C-FLs).
30641899	3	50	theme	cytotoxic	400:408	arg1	characteristics					352:366	the physicochemical characteristics	332:366	the physicochemical characteristics	332:366	The aim of this study was to investigate the physicochemical characteristics, pharmacokinetics behavior, and cytotoxic efficacy of docetaxel (DTX)-loaded CS-coated FLs (C-FLs).
30641899	9	51	theme	dicetyl	1302:1308	arg1	phosphate					1310:1318	dicetyl phosphate	1302:1318	dicetyl phosphate (DP)	1302:1323	The optimized C-FL containing sodium deoxycholate (NDC) and dicetyl phosphate (DP) elicited enhanced pharmacokinetic parameters and cytotoxic efficiency compared to those of the uncoated ones and Onkotaxel®.
30641899	9	51	theme	dicetyl	1302:1308	arg1	DP					1321:1322	DP	1321:1322	DP	1321:1322	The optimized C-FL containing sodium deoxycholate (NDC) and dicetyl phosphate (DP) elicited enhanced pharmacokinetic parameters and cytotoxic efficiency compared to those of the uncoated ones and Onkotaxel®.
30641899	5	52	theme	efficiency	685:694	arg1	profiles					731:738	the particle size, zeta potential, encapsulation efficiency (EE%), morphology, and DTX release profiles	636:738	the particle size, zeta potential, encapsulation efficiency (EE%), morphology, and DTX release profiles	636:738	To explore their physicochemical characterization, the particle size, zeta potential, encapsulation efficiency (EE%), morphology, and DTX release profiles were determined.
30641899	1	53	theme	Flexible	124:131	arg1	FLs					144:146	FLs	144:146	FLs	144:146	Flexible liposomes (FLs) were developed as promising nano-carriers for anticancer drugs.
30641899	1	53	theme	Flexible	124:131	arg1	liposomes					133:141	Flexible liposomes	124:141	Flexible liposomes (FLs)	124:147	Flexible liposomes (FLs) were developed as promising nano-carriers for anticancer drugs.
30641899	1	53	theme	Flexible	124:131	arg1	nano-carriers					177:189	promising nano-carriers	167:189	promising nano-carriers for anticancer drugs	167:210	Flexible liposomes (FLs) were developed as promising nano-carriers for anticancer drugs.
30641899	5	54	theme	EE	697:698	arg1	efficiency					685:694	encapsulation efficiency	671:694	encapsulation efficiency (EE%)	671:700	To explore their physicochemical characterization, the particle size, zeta potential, encapsulation efficiency (EE%), morphology, and DTX release profiles were determined.
30641899	5	54	theme	EE	697:698	arg1	%					699:699	EE%	697:699	EE%	697:699	To explore their physicochemical characterization, the particle size, zeta potential, encapsulation efficiency (EE%), morphology, and DTX release profiles were determined.
30641899	9	55	contain	containing	1261:1270	arg2	DP					1321:1322	DP	1321:1322	DP	1321:1322	The optimized C-FL containing sodium deoxycholate (NDC) and dicetyl phosphate (DP) elicited enhanced pharmacokinetic parameters and cytotoxic efficiency compared to those of the uncoated ones and Onkotaxel®.
30641899	9	55	contain	containing	1261:1270	arg2	NDC					1293:1295	NDC	1293:1295	NDC	1293:1295	The optimized C-FL containing sodium deoxycholate (NDC) and dicetyl phosphate (DP) elicited enhanced pharmacokinetic parameters and cytotoxic efficiency compared to those of the uncoated ones and Onkotaxel®.
30641899	9	55	contain	containing	1261:1270	arg2	phosphate					1310:1318	dicetyl phosphate	1302:1318	dicetyl phosphate (DP)	1302:1323	The optimized C-FL containing sodium deoxycholate (NDC) and dicetyl phosphate (DP) elicited enhanced pharmacokinetic parameters and cytotoxic efficiency compared to those of the uncoated ones and Onkotaxel®.
30641899	9	55	contain	containing	1261:1270	arg1	C-FL					1256:1259	The optimized C-FL	1242:1259	The optimized C-FL containing sodium deoxycholate (NDC) and dicetyl phosphate (DP)	1242:1323	The optimized C-FL containing sodium deoxycholate (NDC) and dicetyl phosphate (DP) elicited enhanced pharmacokinetic parameters and cytotoxic efficiency compared to those of the uncoated ones and Onkotaxel®.
30641899	9	55	contain	containing	1261:1270	arg2	deoxycholate					1279:1290	sodium deoxycholate	1272:1290	sodium deoxycholate (NDC)	1272:1296	The optimized C-FL containing sodium deoxycholate (NDC) and dicetyl phosphate (DP) elicited enhanced pharmacokinetic parameters and cytotoxic efficiency compared to those of the uncoated ones and Onkotaxel®.
30641899	8	56	theme	sustained-release	1169:1185	arg1	evident					1233:1239	evident	1233:1239	evident	1233:1239	Moreover, the zeta potential shifted from a negative to a positive value for C-FL with increased particle size and EE%, and the in vitro sustained-release profiles of C-FL compared to those of FL were evident.
30641899	8	56	theme	sustained-release	1169:1185	arg1	profiles					1187:1194	the in vitro sustained-release profiles	1156:1194	the in vitro sustained-release profiles of C-FL compared to those of FL	1156:1226	Moreover, the zeta potential shifted from a negative to a positive value for C-FL with increased particle size and EE%, and the in vitro sustained-release profiles of C-FL compared to those of FL were evident.
30641899	7	57	theme	dependent	890:898	arg1	FLs					885:887	Various FLs	877:887	Various FLs	877:887	Various FLs, dependent on the type of surfactant, were formed with particle sizes in the nano-range, 137.6 ± 6.3 to 238.2 ± 14.2 nm, and an EE% of 59⁻94%.
30641899	9	58	theme	optimized	1246:1254	arg1	C-FL					1256:1259	The optimized C-FL	1242:1259	The optimized C-FL containing sodium deoxycholate (NDC) and dicetyl phosphate (DP)	1242:1323	The optimized C-FL containing sodium deoxycholate (NDC) and dicetyl phosphate (DP) elicited enhanced pharmacokinetic parameters and cytotoxic efficiency compared to those of the uncoated ones and Onkotaxel®.
30641899	9	59	theme	cytotoxic	1374:1382	arg1	efficiency					1384:1393	cytotoxic efficiency	1374:1393	cytotoxic efficiency	1374:1393	The optimized C-FL containing sodium deoxycholate (NDC) and dicetyl phosphate (DP) elicited enhanced pharmacokinetic parameters and cytotoxic efficiency compared to those of the uncoated ones and Onkotaxel®.
30641899	7	60	theme	Various	877:883	arg1	FLs					885:887	Various FLs	877:887	Various FLs	877:887	Various FLs, dependent on the type of surfactant, were formed with particle sizes in the nano-range, 137.6 ± 6.3 to 238.2 ± 14.2 nm, and an EE% of 59⁻94%.
30641899	7	61	dep	238.2	993:997	arg1	to					990:991	to	990:991	to	990:991	Various FLs, dependent on the type of surfactant, were formed with particle sizes in the nano-range, 137.6 ± 6.3 to 238.2 ± 14.2 nm, and an EE% of 59⁻94%.
30641899	9	62	theme	enhanced	1334:1341	arg1	parameters					1359:1368	enhanced pharmacokinetic parameters	1334:1368	enhanced pharmacokinetic parameters	1334:1368	The optimized C-FL containing sodium deoxycholate (NDC) and dicetyl phosphate (DP) elicited enhanced pharmacokinetic parameters and cytotoxic efficiency compared to those of the uncoated ones and Onkotaxel®.
30641899	0	63	from	Impact	101:106	arg1	Composition					111:121	Composition	111:121	Composition	111:121	Chitosan-Coated Flexible Liposomes Magnify the Anticancer Activity and Bioavailability of Docetaxel: Impact on Composition.
30641899	8	64	dep	in	1160:1161	arg1	vitro					1163:1167	vitro	1163:1167	vitro	1163:1167	Moreover, the zeta potential shifted from a negative to a positive value for C-FL with increased particle size and EE%, and the in vitro sustained-release profiles of C-FL compared to those of FL were evident.
30641899	8	65	with	C-FL	1109:1112	arg1	size					1138:1141	increased particle size	1119:1141	increased particle size	1119:1141	Moreover, the zeta potential shifted from a negative to a positive value for C-FL with increased particle size and EE%, and the in vitro sustained-release profiles of C-FL compared to those of FL were evident.
30641899	8	65	with	C-FL	1109:1112	arg1	%					1149:1149	EE%	1147:1149	EE%	1147:1149	Moreover, the zeta potential shifted from a negative to a positive value for C-FL with increased particle size and EE%, and the in vitro sustained-release profiles of C-FL compared to those of FL were evident.
30641899	8	66	theme	increased	1119:1127	arg1	size					1138:1141	increased particle size	1119:1141	increased particle size	1119:1141	Moreover, the zeta potential shifted from a negative to a positive value for C-FL with increased particle size and EE%, and the in vitro sustained-release profiles of C-FL compared to those of FL were evident.
30641899	9	67	theme	sodium	1272:1277	arg1	NDC					1293:1295	NDC	1293:1295	NDC	1293:1295	The optimized C-FL containing sodium deoxycholate (NDC) and dicetyl phosphate (DP) elicited enhanced pharmacokinetic parameters and cytotoxic efficiency compared to those of the uncoated ones and Onkotaxel®.
30641899	9	67	theme	sodium	1272:1277	arg1	deoxycholate					1279:1290	sodium deoxycholate	1272:1290	sodium deoxycholate (NDC)	1272:1296	The optimized C-FL containing sodium deoxycholate (NDC) and dicetyl phosphate (DP) elicited enhanced pharmacokinetic parameters and cytotoxic efficiency compared to those of the uncoated ones and Onkotaxel®.
30537806	3	0	theme	enteric	671:677	arg1	material					696:703	enteric Eudragit L100-55 material	671:703	enteric Eudragit L100-55 material	671:703	Herein, we used insulin as a model protein drug; insulin-loaded N-(2-hydroxy)-propyl-3-trimethylammonium chloride modified chitosan (HTCC)/sodium tripolyphosphate (TPP) nanocomplex (NC) as a nanocore was further encapsulated into enteric Eudragit L100-55 material, through a two-step flash nanocomplexation (FNC) process in a reliable and scalable manner, forming our NC-in-Eudragit composite particles (NE).
30537806	3	1	theme	N-	505:506	arg1	chloride					546:553	insulin-loaded N-(2-hydroxy)-propyl-3-trimethylammonium chloride	490:553	insulin-loaded N-(2-hydroxy)-propyl-3-trimethylammonium chloride modified chitosan (HTCC)/sodium tripolyphosphate (TPP) nanocomplex (NC) as a nanocore	490:639	Herein, we used insulin as a model protein drug; insulin-loaded N-(2-hydroxy)-propyl-3-trimethylammonium chloride modified chitosan (HTCC)/sodium tripolyphosphate (TPP) nanocomplex (NC) as a nanocore was further encapsulated into enteric Eudragit L100-55 material, through a two-step flash nanocomplexation (FNC) process in a reliable and scalable manner, forming our NC-in-Eudragit composite particles (NE).
30537806	5	2	theme	optimized	1174:1182	arg1	NE					1184:1185	the optimized NE	1170:1185	the optimized NE	1170:1185	In addition, the oral administration of the optimized NE to type 1 diabetic rats could induce a very significant hypoglycemic effect with a relative oral bioavailability of 13.3%.
30537806	4	3	theme	site-specific	1036:1048	arg1	release					1055:1061	intestinal site-specific drug release	1025:1061	intestinal site-specific drug release as well as the improvement of oral delivery efficiency of insulin	1025:1127	Particle size and surface properties of our optimized NE were tailored to protect the loaded insulin from acidic degradation in the hostile stomach environment and to achieve intestinal site-specific drug release as well as the improvement of oral delivery efficiency of insulin.
30537806	3	4	theme	modified	555:562	arg1	nanocomplex					610:620	insulin-loaded N-(2-hydroxy)-propyl-3-trimethylammonium chloride modified chitosan (HTCC)/sodium tripolyphosphate (TPP) nanocomplex	490:620	insulin-loaded N-(2-hydroxy)-propyl-3-trimethylammonium chloride modified chitosan (HTCC)/sodium tripolyphosphate (TPP) nanocomplex (NC) as a nanocore	490:639	Herein, we used insulin as a model protein drug; insulin-loaded N-(2-hydroxy)-propyl-3-trimethylammonium chloride modified chitosan (HTCC)/sodium tripolyphosphate (TPP) nanocomplex (NC) as a nanocore was further encapsulated into enteric Eudragit L100-55 material, through a two-step flash nanocomplexation (FNC) process in a reliable and scalable manner, forming our NC-in-Eudragit composite particles (NE).
30537806	3	4	theme	modified	555:562	arg1	NC					623:624	NC	623:624	NC	623:624	Herein, we used insulin as a model protein drug; insulin-loaded N-(2-hydroxy)-propyl-3-trimethylammonium chloride modified chitosan (HTCC)/sodium tripolyphosphate (TPP) nanocomplex (NC) as a nanocore was further encapsulated into enteric Eudragit L100-55 material, through a two-step flash nanocomplexation (FNC) process in a reliable and scalable manner, forming our NC-in-Eudragit composite particles (NE).
30537806	4	5	theme	delivery	1098:1105	arg1	efficiency					1107:1116	oral delivery efficiency	1093:1116	oral delivery efficiency of insulin	1093:1127	Particle size and surface properties of our optimized NE were tailored to protect the loaded insulin from acidic degradation in the hostile stomach environment and to achieve intestinal site-specific drug release as well as the improvement of oral delivery efficiency of insulin.
30537806	3	6	theme	-propyl-3-trimethylammonium	518:544	arg1	chloride					546:553	insulin-loaded N-(2-hydroxy)-propyl-3-trimethylammonium chloride	490:553	insulin-loaded N-(2-hydroxy)-propyl-3-trimethylammonium chloride modified chitosan (HTCC)/sodium tripolyphosphate (TPP) nanocomplex (NC) as a nanocore	490:639	Herein, we used insulin as a model protein drug; insulin-loaded N-(2-hydroxy)-propyl-3-trimethylammonium chloride modified chitosan (HTCC)/sodium tripolyphosphate (TPP) nanocomplex (NC) as a nanocore was further encapsulated into enteric Eudragit L100-55 material, through a two-step flash nanocomplexation (FNC) process in a reliable and scalable manner, forming our NC-in-Eudragit composite particles (NE).
30537806	6	7	theme	surface	1518:1524	arg1	coating					1526:1532	homogeneous surface coating	1506:1532	homogeneous surface coating	1506:1532	Our results demonstrated that enteric encapsulation of nanotherapeutics using a FNC apparatus could cause drug formulations to possess better size controllability, batch-mode reproducibility, and homogeneous surface coating and then significantly enhance their oral bioavailability of insulin, indicating its great potential for clinical translation of oral protein therapeutics.
30537806	1	8	theme	administration	180:193	arg1	ease					172:175	its ease	168:175	its ease of administration	168:193	Oral drug delivery is a more favored mode of administration because of its ease of administration, high patient compliance, and low healthcare costs.
30537806	1	8	theme	administration	180:193	arg1	costs					240:244	low healthcare costs	225:244	low healthcare costs	225:244	Oral drug delivery is a more favored mode of administration because of its ease of administration, high patient compliance, and low healthcare costs.
30537806	1	8	theme	administration	180:193	arg1	compliance					209:218	high patient compliance	196:218	high patient compliance	196:218	Oral drug delivery is a more favored mode of administration because of its ease of administration, high patient compliance, and low healthcare costs.
30537806	4	9	theme	optimized	894:902	arg1	NE					904:905	our optimized NE	890:905	our optimized NE	890:905	Particle size and surface properties of our optimized NE were tailored to protect the loaded insulin from acidic degradation in the hostile stomach environment and to achieve intestinal site-specific drug release as well as the improvement of oral delivery efficiency of insulin.
30537806	3	10	theme	flash	725:729	arg1	process					754:760	a two-step flash nanocomplexation (FNC) process	714:760	a two-step flash nanocomplexation (FNC) process in a reliable and scalable manner	714:794	Herein, we used insulin as a model protein drug; insulin-loaded N-(2-hydroxy)-propyl-3-trimethylammonium chloride modified chitosan (HTCC)/sodium tripolyphosphate (TPP) nanocomplex (NC) as a nanocore was further encapsulated into enteric Eudragit L100-55 material, through a two-step flash nanocomplexation (FNC) process in a reliable and scalable manner, forming our NC-in-Eudragit composite particles (NE).
30537806	1	11	theme	high	196:199	arg1	compliance					209:218	high patient compliance	196:218	high patient compliance	196:218	Oral drug delivery is a more favored mode of administration because of its ease of administration, high patient compliance, and low healthcare costs.
30537806	3	12	theme	TPP	605:607	arg1	nanocomplex					610:620	insulin-loaded N-(2-hydroxy)-propyl-3-trimethylammonium chloride modified chitosan (HTCC)/sodium tripolyphosphate (TPP) nanocomplex	490:620	insulin-loaded N-(2-hydroxy)-propyl-3-trimethylammonium chloride modified chitosan (HTCC)/sodium tripolyphosphate (TPP) nanocomplex (NC) as a nanocore	490:639	Herein, we used insulin as a model protein drug; insulin-loaded N-(2-hydroxy)-propyl-3-trimethylammonium chloride modified chitosan (HTCC)/sodium tripolyphosphate (TPP) nanocomplex (NC) as a nanocore was further encapsulated into enteric Eudragit L100-55 material, through a two-step flash nanocomplexation (FNC) process in a reliable and scalable manner, forming our NC-in-Eudragit composite particles (NE).
30537806	3	12	theme	TPP	605:607	arg1	NC					623:624	NC	623:624	NC	623:624	Herein, we used insulin as a model protein drug; insulin-loaded N-(2-hydroxy)-propyl-3-trimethylammonium chloride modified chitosan (HTCC)/sodium tripolyphosphate (TPP) nanocomplex (NC) as a nanocore was further encapsulated into enteric Eudragit L100-55 material, through a two-step flash nanocomplexation (FNC) process in a reliable and scalable manner, forming our NC-in-Eudragit composite particles (NE).
30537806	4	13	theme	surface	868:874	arg1	properties					876:885	surface properties	868:885	surface properties	868:885	Particle size and surface properties of our optimized NE were tailored to protect the loaded insulin from acidic degradation in the hostile stomach environment and to achieve intestinal site-specific drug release as well as the improvement of oral delivery efficiency of insulin.
30537806	6	14	theme	drug	1416:1419	arg1	formulations					1421:1432	drug formulations	1416:1432	drug formulations	1416:1432	Our results demonstrated that enteric encapsulation of nanotherapeutics using a FNC apparatus could cause drug formulations to possess better size controllability, batch-mode reproducibility, and homogeneous surface coating and then significantly enhance their oral bioavailability of insulin, indicating its great potential for clinical translation of oral protein therapeutics.
30537806	3	15	theme	tripolyphosphate	587:602	arg1	nanocomplex					610:620	insulin-loaded N-(2-hydroxy)-propyl-3-trimethylammonium chloride modified chitosan (HTCC)/sodium tripolyphosphate (TPP) nanocomplex	490:620	insulin-loaded N-(2-hydroxy)-propyl-3-trimethylammonium chloride modified chitosan (HTCC)/sodium tripolyphosphate (TPP) nanocomplex (NC) as a nanocore	490:639	Herein, we used insulin as a model protein drug; insulin-loaded N-(2-hydroxy)-propyl-3-trimethylammonium chloride modified chitosan (HTCC)/sodium tripolyphosphate (TPP) nanocomplex (NC) as a nanocore was further encapsulated into enteric Eudragit L100-55 material, through a two-step flash nanocomplexation (FNC) process in a reliable and scalable manner, forming our NC-in-Eudragit composite particles (NE).
30537806	3	15	theme	tripolyphosphate	587:602	arg1	NC					623:624	NC	623:624	NC	623:624	Herein, we used insulin as a model protein drug; insulin-loaded N-(2-hydroxy)-propyl-3-trimethylammonium chloride modified chitosan (HTCC)/sodium tripolyphosphate (TPP) nanocomplex (NC) as a nanocore was further encapsulated into enteric Eudragit L100-55 material, through a two-step flash nanocomplexation (FNC) process in a reliable and scalable manner, forming our NC-in-Eudragit composite particles (NE).
30537806	6	16	theme	insulin	1595:1601	arg1	bioavailability					1576:1590	their oral bioavailability	1565:1590	their oral bioavailability of insulin	1565:1601	Our results demonstrated that enteric encapsulation of nanotherapeutics using a FNC apparatus could cause drug formulations to possess better size controllability, batch-mode reproducibility, and homogeneous surface coating and then significantly enhance their oral bioavailability of insulin, indicating its great potential for clinical translation of oral protein therapeutics.
30537806	5	17	theme	hypoglycemic	1243:1254	arg1	effect					1256:1261	a very significant hypoglycemic effect	1224:1261	a very significant hypoglycemic effect	1224:1261	In addition, the oral administration of the optimized NE to type 1 diabetic rats could induce a very significant hypoglycemic effect with a relative oral bioavailability of 13.3%.
30537806	3	18	theme	scalable	780:787	arg1	manner					789:794	a reliable and scalable manner	765:794	a reliable and scalable manner	765:794	Herein, we used insulin as a model protein drug; insulin-loaded N-(2-hydroxy)-propyl-3-trimethylammonium chloride modified chitosan (HTCC)/sodium tripolyphosphate (TPP) nanocomplex (NC) as a nanocore was further encapsulated into enteric Eudragit L100-55 material, through a two-step flash nanocomplexation (FNC) process in a reliable and scalable manner, forming our NC-in-Eudragit composite particles (NE).
30537806	3	19	theme	composite	824:832	arg1	NE					845:846	NE	845:846	NE	845:846	Herein, we used insulin as a model protein drug; insulin-loaded N-(2-hydroxy)-propyl-3-trimethylammonium chloride modified chitosan (HTCC)/sodium tripolyphosphate (TPP) nanocomplex (NC) as a nanocore was further encapsulated into enteric Eudragit L100-55 material, through a two-step flash nanocomplexation (FNC) process in a reliable and scalable manner, forming our NC-in-Eudragit composite particles (NE).
30537806	3	19	theme	composite	824:832	arg1	particles					834:842	our NC-in-Eudragit composite particles	805:842	our NC-in-Eudragit composite particles (NE)	805:847	Herein, we used insulin as a model protein drug; insulin-loaded N-(2-hydroxy)-propyl-3-trimethylammonium chloride modified chitosan (HTCC)/sodium tripolyphosphate (TPP) nanocomplex (NC) as a nanocore was further encapsulated into enteric Eudragit L100-55 material, through a two-step flash nanocomplexation (FNC) process in a reliable and scalable manner, forming our NC-in-Eudragit composite particles (NE).
30537806	6	20	theme	protein	1668:1674	arg1	therapeutics					1676:1687	oral protein therapeutics	1663:1687	oral protein therapeutics	1663:1687	Our results demonstrated that enteric encapsulation of nanotherapeutics using a FNC apparatus could cause drug formulations to possess better size controllability, batch-mode reproducibility, and homogeneous surface coating and then significantly enhance their oral bioavailability of insulin, indicating its great potential for clinical translation of oral protein therapeutics.
30537806	3	21	theme	reliable	767:774	arg1	manner					789:794	a reliable and scalable manner	765:794	a reliable and scalable manner	765:794	Herein, we used insulin as a model protein drug; insulin-loaded N-(2-hydroxy)-propyl-3-trimethylammonium chloride modified chitosan (HTCC)/sodium tripolyphosphate (TPP) nanocomplex (NC) as a nanocore was further encapsulated into enteric Eudragit L100-55 material, through a two-step flash nanocomplexation (FNC) process in a reliable and scalable manner, forming our NC-in-Eudragit composite particles (NE).
30537806	0	22	theme	Oral	74:77	arg1	Delivery					87:94	Site-Specific Oral Insulin Delivery	60:94	Site-Specific Oral Insulin Delivery	60:94	Scalable Manufacturing of Enteric Encapsulation Systems for Site-Specific Oral Insulin Delivery.
30537806	1	23	theme	administration	142:155	arg1	mode					134:137	a more favored mode	119:137	a more favored mode of administration	119:155	Oral drug delivery is a more favored mode of administration because of its ease of administration, high patient compliance, and low healthcare costs.
30537806	1	23	theme	administration	142:155	arg1	delivery					107:114	Oral drug delivery	97:114	Oral drug delivery	97:114	Oral drug delivery is a more favored mode of administration because of its ease of administration, high patient compliance, and low healthcare costs.
30537806	4	24	theme	acidic	956:961	arg1	degradation					963:973	acidic degradation	956:973	acidic degradation in the hostile stomach environment	956:1008	Particle size and surface properties of our optimized NE were tailored to protect the loaded insulin from acidic degradation in the hostile stomach environment and to achieve intestinal site-specific drug release as well as the improvement of oral delivery efficiency of insulin.
30537806	6	25	theme	enteric	1340:1346	arg1	encapsulation					1348:1360	enteric encapsulation	1340:1360	enteric encapsulation of nanotherapeutics using a FNC apparatus	1340:1402	Our results demonstrated that enteric encapsulation of nanotherapeutics using a FNC apparatus could cause drug formulations to possess better size controllability, batch-mode reproducibility, and homogeneous surface coating and then significantly enhance their oral bioavailability of insulin, indicating its great potential for clinical translation of oral protein therapeutics.
30537806	6	26	theme	oral	1663:1666	arg1	therapeutics					1676:1687	oral protein therapeutics	1663:1687	oral protein therapeutics	1663:1687	Our results demonstrated that enteric encapsulation of nanotherapeutics using a FNC apparatus could cause drug formulations to possess better size controllability, batch-mode reproducibility, and homogeneous surface coating and then significantly enhance their oral bioavailability of insulin, indicating its great potential for clinical translation of oral protein therapeutics.
30537806	2	27	theme	protein	264:270	arg1	formulations					272:283	no oral protein formulations	256:283	no oral protein formulations	256:283	However, no oral protein formulations are commercially available currently due to hostile gastrointestinal (GI) barriers resulting in insignificant oral bioavailability of macromolecular drugs.
30537806	2	28	theme	oral	395:398	arg1	bioavailability					400:414	insignificant oral bioavailability	381:414	insignificant oral bioavailability of macromolecular drugs	381:438	However, no oral protein formulations are commercially available currently due to hostile gastrointestinal (GI) barriers resulting in insignificant oral bioavailability of macromolecular drugs.
30537806	0	29	theme	Insulin	79:85	arg1	Delivery					87:94	Site-Specific Oral Insulin Delivery	60:94	Site-Specific Oral Insulin Delivery	60:94	Scalable Manufacturing of Enteric Encapsulation Systems for Site-Specific Oral Insulin Delivery.
30537806	0	30	theme	Scalable	0:7	arg1	Manufacturing					9:21	Scalable Manufacturing	0:21	Scalable Manufacturing of Enteric Encapsulation Systems for Site-Specific Oral Insulin Delivery.	0:95	Scalable Manufacturing of Enteric Encapsulation Systems for Site-Specific Oral Insulin Delivery.
30537806	6	31	theme	great	1619:1623	arg1	potential					1625:1633	its great potential	1615:1633	its great potential for clinical translation of oral protein therapeutics	1615:1687	Our results demonstrated that enteric encapsulation of nanotherapeutics using a FNC apparatus could cause drug formulations to possess better size controllability, batch-mode reproducibility, and homogeneous surface coating and then significantly enhance their oral bioavailability of insulin, indicating its great potential for clinical translation of oral protein therapeutics.
30537806	1	32	theme	drug	102:105	arg1	mode					134:137	a more favored mode	119:137	a more favored mode of administration	119:155	Oral drug delivery is a more favored mode of administration because of its ease of administration, high patient compliance, and low healthcare costs.
30537806	1	32	theme	drug	102:105	arg1	delivery					107:114	Oral drug delivery	97:114	Oral drug delivery	97:114	Oral drug delivery is a more favored mode of administration because of its ease of administration, high patient compliance, and low healthcare costs.
30537806	2	33	dep	gastrointestinal	337:352	arg1	GI					355:356	GI	355:356	GI	355:356	However, no oral protein formulations are commercially available currently due to hostile gastrointestinal (GI) barriers resulting in insignificant oral bioavailability of macromolecular drugs.
30537806	5	34	theme	relative	1270:1277	arg1	bioavailability					1284:1298	a relative oral bioavailability	1268:1298	a relative oral bioavailability of 13.3%	1268:1307	In addition, the oral administration of the optimized NE to type 1 diabetic rats could induce a very significant hypoglycemic effect with a relative oral bioavailability of 13.3%.
30537806	2	35	theme	drugs	434:438	arg1	bioavailability					400:414	insignificant oral bioavailability	381:414	insignificant oral bioavailability of macromolecular drugs	381:438	However, no oral protein formulations are commercially available currently due to hostile gastrointestinal (GI) barriers resulting in insignificant oral bioavailability of macromolecular drugs.
30537806	6	36	theme	FNC	1390:1392	arg1	apparatus					1394:1402	a FNC apparatus	1388:1402	a FNC apparatus	1388:1402	Our results demonstrated that enteric encapsulation of nanotherapeutics using a FNC apparatus could cause drug formulations to possess better size controllability, batch-mode reproducibility, and homogeneous surface coating and then significantly enhance their oral bioavailability of insulin, indicating its great potential for clinical translation of oral protein therapeutics.
30537806	0	37	theme	Encapsulation	34:46	arg1	Systems					48:54	Enteric Encapsulation Systems	26:54	Enteric Encapsulation Systems	26:54	Scalable Manufacturing of Enteric Encapsulation Systems for Site-Specific Oral Insulin Delivery.
30537806	4	38	theme	hostile	982:988	arg1	environment					998:1008	the hostile stomach environment	978:1008	the hostile stomach environment	978:1008	Particle size and surface properties of our optimized NE were tailored to protect the loaded insulin from acidic degradation in the hostile stomach environment and to achieve intestinal site-specific drug release as well as the improvement of oral delivery efficiency of insulin.
30537806	2	39	theme	gastrointestinal	337:352	arg1	barriers					359:366	hostile gastrointestinal (GI) barriers	329:366	hostile gastrointestinal (GI) barriers resulting in insignificant oral bioavailability of macromolecular drugs	329:438	However, no oral protein formulations are commercially available currently due to hostile gastrointestinal (GI) barriers resulting in insignificant oral bioavailability of macromolecular drugs.
30537806	6	40	contain	possess	1437:1443	arg2	reproducibility					1485:1499	batch-mode reproducibility	1474:1499	batch-mode reproducibility	1474:1499	Our results demonstrated that enteric encapsulation of nanotherapeutics using a FNC apparatus could cause drug formulations to possess better size controllability, batch-mode reproducibility, and homogeneous surface coating and then significantly enhance their oral bioavailability of insulin, indicating its great potential for clinical translation of oral protein therapeutics.
30537806	6	40	contain	possess	1437:1443	arg2	coating					1526:1532	homogeneous surface coating	1506:1532	homogeneous surface coating	1506:1532	Our results demonstrated that enteric encapsulation of nanotherapeutics using a FNC apparatus could cause drug formulations to possess better size controllability, batch-mode reproducibility, and homogeneous surface coating and then significantly enhance their oral bioavailability of insulin, indicating its great potential for clinical translation of oral protein therapeutics.
30537806	6	40	contain	possess	1437:1443	arg2	controllability					1457:1471	better size controllability	1445:1471	better size controllability	1445:1471	Our results demonstrated that enteric encapsulation of nanotherapeutics using a FNC apparatus could cause drug formulations to possess better size controllability, batch-mode reproducibility, and homogeneous surface coating and then significantly enhance their oral bioavailability of insulin, indicating its great potential for clinical translation of oral protein therapeutics.
30537806	6	40	contain	possess	1437:1443	arg1	formulations					1421:1432	drug formulations	1416:1432	drug formulations	1416:1432	Our results demonstrated that enteric encapsulation of nanotherapeutics using a FNC apparatus could cause drug formulations to possess better size controllability, batch-mode reproducibility, and homogeneous surface coating and then significantly enhance their oral bioavailability of insulin, indicating its great potential for clinical translation of oral protein therapeutics.
30537806	3	41	theme	model	470:474	arg1	drug					484:487	a model protein drug	468:487	a model protein drug	468:487	Herein, we used insulin as a model protein drug; insulin-loaded N-(2-hydroxy)-propyl-3-trimethylammonium chloride modified chitosan (HTCC)/sodium tripolyphosphate (TPP) nanocomplex (NC) as a nanocore was further encapsulated into enteric Eudragit L100-55 material, through a two-step flash nanocomplexation (FNC) process in a reliable and scalable manner, forming our NC-in-Eudragit composite particles (NE).
30537806	3	41	theme	model	470:474	arg1	insulin					457:463	insulin	457:463	insulin	457:463	Herein, we used insulin as a model protein drug; insulin-loaded N-(2-hydroxy)-propyl-3-trimethylammonium chloride modified chitosan (HTCC)/sodium tripolyphosphate (TPP) nanocomplex (NC) as a nanocore was further encapsulated into enteric Eudragit L100-55 material, through a two-step flash nanocomplexation (FNC) process in a reliable and scalable manner, forming our NC-in-Eudragit composite particles (NE).
30537806	6	42	theme	batch-mode	1474:1483	arg1	reproducibility					1485:1499	batch-mode reproducibility	1474:1499	batch-mode reproducibility	1474:1499	Our results demonstrated that enteric encapsulation of nanotherapeutics using a FNC apparatus could cause drug formulations to possess better size controllability, batch-mode reproducibility, and homogeneous surface coating and then significantly enhance their oral bioavailability of insulin, indicating its great potential for clinical translation of oral protein therapeutics.
30537806	5	43	theme	diabetic	1197:1204	arg1	rats					1206:1209	type 1 diabetic rats	1190:1209	type 1 diabetic rats	1190:1209	In addition, the oral administration of the optimized NE to type 1 diabetic rats could induce a very significant hypoglycemic effect with a relative oral bioavailability of 13.3%.
30537806	4	44	theme	intestinal	1025:1034	arg1	release					1055:1061	intestinal site-specific drug release	1025:1061	intestinal site-specific drug release as well as the improvement of oral delivery efficiency of insulin	1025:1127	Particle size and surface properties of our optimized NE were tailored to protect the loaded insulin from acidic degradation in the hostile stomach environment and to achieve intestinal site-specific drug release as well as the improvement of oral delivery efficiency of insulin.
30537806	3	45	theme	insulin-loaded	490:503	arg1	chloride					546:553	insulin-loaded N-(2-hydroxy)-propyl-3-trimethylammonium chloride	490:553	insulin-loaded N-(2-hydroxy)-propyl-3-trimethylammonium chloride modified chitosan (HTCC)/sodium tripolyphosphate (TPP) nanocomplex (NC) as a nanocore	490:639	Herein, we used insulin as a model protein drug; insulin-loaded N-(2-hydroxy)-propyl-3-trimethylammonium chloride modified chitosan (HTCC)/sodium tripolyphosphate (TPP) nanocomplex (NC) as a nanocore was further encapsulated into enteric Eudragit L100-55 material, through a two-step flash nanocomplexation (FNC) process in a reliable and scalable manner, forming our NC-in-Eudragit composite particles (NE).
30537806	6	46	theme	better	1445:1450	arg1	controllability					1457:1471	better size controllability	1445:1471	better size controllability	1445:1471	Our results demonstrated that enteric encapsulation of nanotherapeutics using a FNC apparatus could cause drug formulations to possess better size controllability, batch-mode reproducibility, and homogeneous surface coating and then significantly enhance their oral bioavailability of insulin, indicating its great potential for clinical translation of oral protein therapeutics.
30537806	4	47	theme	drug	1050:1053	arg1	release					1055:1061	intestinal site-specific drug release	1025:1061	intestinal site-specific drug release as well as the improvement of oral delivery efficiency of insulin	1025:1127	Particle size and surface properties of our optimized NE were tailored to protect the loaded insulin from acidic degradation in the hostile stomach environment and to achieve intestinal site-specific drug release as well as the improvement of oral delivery efficiency of insulin.
30537806	3	48	theme	Eudragit	679:686	arg1	material					696:703	enteric Eudragit L100-55 material	671:703	enteric Eudragit L100-55 material	671:703	Herein, we used insulin as a model protein drug; insulin-loaded N-(2-hydroxy)-propyl-3-trimethylammonium chloride modified chitosan (HTCC)/sodium tripolyphosphate (TPP) nanocomplex (NC) as a nanocore was further encapsulated into enteric Eudragit L100-55 material, through a two-step flash nanocomplexation (FNC) process in a reliable and scalable manner, forming our NC-in-Eudragit composite particles (NE).
30537806	3	49	theme	chloride	546:553	arg1	nanocomplex					610:620	insulin-loaded N-(2-hydroxy)-propyl-3-trimethylammonium chloride modified chitosan (HTCC)/sodium tripolyphosphate (TPP) nanocomplex	490:620	insulin-loaded N-(2-hydroxy)-propyl-3-trimethylammonium chloride modified chitosan (HTCC)/sodium tripolyphosphate (TPP) nanocomplex (NC) as a nanocore	490:639	Herein, we used insulin as a model protein drug; insulin-loaded N-(2-hydroxy)-propyl-3-trimethylammonium chloride modified chitosan (HTCC)/sodium tripolyphosphate (TPP) nanocomplex (NC) as a nanocore was further encapsulated into enteric Eudragit L100-55 material, through a two-step flash nanocomplexation (FNC) process in a reliable and scalable manner, forming our NC-in-Eudragit composite particles (NE).
30537806	3	49	theme	chloride	546:553	arg1	NC					623:624	NC	623:624	NC	623:624	Herein, we used insulin as a model protein drug; insulin-loaded N-(2-hydroxy)-propyl-3-trimethylammonium chloride modified chitosan (HTCC)/sodium tripolyphosphate (TPP) nanocomplex (NC) as a nanocore was further encapsulated into enteric Eudragit L100-55 material, through a two-step flash nanocomplexation (FNC) process in a reliable and scalable manner, forming our NC-in-Eudragit composite particles (NE).
30537806	5	50	theme	NE	1184:1185	arg1	administration					1152:1165	the oral administration	1143:1165	the oral administration of the optimized NE to type 1 diabetic rats	1143:1209	In addition, the oral administration of the optimized NE to type 1 diabetic rats could induce a very significant hypoglycemic effect with a relative oral bioavailability of 13.3%.
30537806	4	51	theme	Particle	850:857	arg1	size					859:862	Particle size	850:862	Particle size	850:862	Particle size and surface properties of our optimized NE were tailored to protect the loaded insulin from acidic degradation in the hostile stomach environment and to achieve intestinal site-specific drug release as well as the improvement of oral delivery efficiency of insulin.
30537806	4	52	theme	efficiency	1107:1116	arg1	improvement					1078:1088	the improvement	1074:1088	intestinal site-specific drug release as well as the improvement of oral delivery efficiency of insulin	1025:1127	Particle size and surface properties of our optimized NE were tailored to protect the loaded insulin from acidic degradation in the hostile stomach environment and to achieve intestinal site-specific drug release as well as the improvement of oral delivery efficiency of insulin.
30537806	4	52	theme	efficiency	1107:1116	arg1	release					1055:1061	intestinal site-specific drug release	1025:1061	intestinal site-specific drug release as well as the improvement of oral delivery efficiency of insulin	1025:1127	Particle size and surface properties of our optimized NE were tailored to protect the loaded insulin from acidic degradation in the hostile stomach environment and to achieve intestinal site-specific drug release as well as the improvement of oral delivery efficiency of insulin.
30537806	3	53	theme	two-step	716:723	arg1	process					754:760	a two-step flash nanocomplexation (FNC) process	714:760	a two-step flash nanocomplexation (FNC) process in a reliable and scalable manner	714:794	Herein, we used insulin as a model protein drug; insulin-loaded N-(2-hydroxy)-propyl-3-trimethylammonium chloride modified chitosan (HTCC)/sodium tripolyphosphate (TPP) nanocomplex (NC) as a nanocore was further encapsulated into enteric Eudragit L100-55 material, through a two-step flash nanocomplexation (FNC) process in a reliable and scalable manner, forming our NC-in-Eudragit composite particles (NE).
30537806	2	54	theme	oral	259:262	arg1	formulations					272:283	no oral protein formulations	256:283	no oral protein formulations	256:283	However, no oral protein formulations are commercially available currently due to hostile gastrointestinal (GI) barriers resulting in insignificant oral bioavailability of macromolecular drugs.
30537806	4	55	theme	insulin	1121:1127	arg1	efficiency					1107:1116	oral delivery efficiency	1093:1116	oral delivery efficiency of insulin	1093:1127	Particle size and surface properties of our optimized NE were tailored to protect the loaded insulin from acidic degradation in the hostile stomach environment and to achieve intestinal site-specific drug release as well as the improvement of oral delivery efficiency of insulin.
30537806	3	56	theme	HTCC	574:577	arg1	nanocomplex					610:620	insulin-loaded N-(2-hydroxy)-propyl-3-trimethylammonium chloride modified chitosan (HTCC)/sodium tripolyphosphate (TPP) nanocomplex	490:620	insulin-loaded N-(2-hydroxy)-propyl-3-trimethylammonium chloride modified chitosan (HTCC)/sodium tripolyphosphate (TPP) nanocomplex (NC) as a nanocore	490:639	Herein, we used insulin as a model protein drug; insulin-loaded N-(2-hydroxy)-propyl-3-trimethylammonium chloride modified chitosan (HTCC)/sodium tripolyphosphate (TPP) nanocomplex (NC) as a nanocore was further encapsulated into enteric Eudragit L100-55 material, through a two-step flash nanocomplexation (FNC) process in a reliable and scalable manner, forming our NC-in-Eudragit composite particles (NE).
30537806	3	56	theme	HTCC	574:577	arg1	NC					623:624	NC	623:624	NC	623:624	Herein, we used insulin as a model protein drug; insulin-loaded N-(2-hydroxy)-propyl-3-trimethylammonium chloride modified chitosan (HTCC)/sodium tripolyphosphate (TPP) nanocomplex (NC) as a nanocore was further encapsulated into enteric Eudragit L100-55 material, through a two-step flash nanocomplexation (FNC) process in a reliable and scalable manner, forming our NC-in-Eudragit composite particles (NE).
30537806	3	57	theme	FNC	749:751	arg1	process					754:760	a two-step flash nanocomplexation (FNC) process	714:760	a two-step flash nanocomplexation (FNC) process in a reliable and scalable manner	714:794	Herein, we used insulin as a model protein drug; insulin-loaded N-(2-hydroxy)-propyl-3-trimethylammonium chloride modified chitosan (HTCC)/sodium tripolyphosphate (TPP) nanocomplex (NC) as a nanocore was further encapsulated into enteric Eudragit L100-55 material, through a two-step flash nanocomplexation (FNC) process in a reliable and scalable manner, forming our NC-in-Eudragit composite particles (NE).
30537806	3	58	theme	chitosan	564:571	arg1	nanocomplex					610:620	insulin-loaded N-(2-hydroxy)-propyl-3-trimethylammonium chloride modified chitosan (HTCC)/sodium tripolyphosphate (TPP) nanocomplex	490:620	insulin-loaded N-(2-hydroxy)-propyl-3-trimethylammonium chloride modified chitosan (HTCC)/sodium tripolyphosphate (TPP) nanocomplex (NC) as a nanocore	490:639	Herein, we used insulin as a model protein drug; insulin-loaded N-(2-hydroxy)-propyl-3-trimethylammonium chloride modified chitosan (HTCC)/sodium tripolyphosphate (TPP) nanocomplex (NC) as a nanocore was further encapsulated into enteric Eudragit L100-55 material, through a two-step flash nanocomplexation (FNC) process in a reliable and scalable manner, forming our NC-in-Eudragit composite particles (NE).
30537806	3	58	theme	chitosan	564:571	arg1	NC					623:624	NC	623:624	NC	623:624	Herein, we used insulin as a model protein drug; insulin-loaded N-(2-hydroxy)-propyl-3-trimethylammonium chloride modified chitosan (HTCC)/sodium tripolyphosphate (TPP) nanocomplex (NC) as a nanocore was further encapsulated into enteric Eudragit L100-55 material, through a two-step flash nanocomplexation (FNC) process in a reliable and scalable manner, forming our NC-in-Eudragit composite particles (NE).
30537806	6	59	theme	homogeneous	1506:1516	arg1	coating					1526:1532	homogeneous surface coating	1506:1532	homogeneous surface coating	1506:1532	Our results demonstrated that enteric encapsulation of nanotherapeutics using a FNC apparatus could cause drug formulations to possess better size controllability, batch-mode reproducibility, and homogeneous surface coating and then significantly enhance their oral bioavailability of insulin, indicating its great potential for clinical translation of oral protein therapeutics.
30537806	4	60	theme	oral	1093:1096	arg1	efficiency					1107:1116	oral delivery efficiency	1093:1116	oral delivery efficiency of insulin	1093:1127	Particle size and surface properties of our optimized NE were tailored to protect the loaded insulin from acidic degradation in the hostile stomach environment and to achieve intestinal site-specific drug release as well as the improvement of oral delivery efficiency of insulin.
30537806	3	61	theme	nanocomplexation	731:746	arg1	process					754:760	a two-step flash nanocomplexation (FNC) process	714:760	a two-step flash nanocomplexation (FNC) process in a reliable and scalable manner	714:794	Herein, we used insulin as a model protein drug; insulin-loaded N-(2-hydroxy)-propyl-3-trimethylammonium chloride modified chitosan (HTCC)/sodium tripolyphosphate (TPP) nanocomplex (NC) as a nanocore was further encapsulated into enteric Eudragit L100-55 material, through a two-step flash nanocomplexation (FNC) process in a reliable and scalable manner, forming our NC-in-Eudragit composite particles (NE).
30537806	1	62	theme	patient	201:207	arg1	compliance					209:218	high patient compliance	196:218	high patient compliance	196:218	Oral drug delivery is a more favored mode of administration because of its ease of administration, high patient compliance, and low healthcare costs.
30537806	4	63	theme	NE	904:905	arg1	properties					876:885	surface properties	868:885	surface properties	868:885	Particle size and surface properties of our optimized NE were tailored to protect the loaded insulin from acidic degradation in the hostile stomach environment and to achieve intestinal site-specific drug release as well as the improvement of oral delivery efficiency of insulin.
30537806	4	63	theme	NE	904:905	arg1	size					859:862	Particle size	850:862	Particle size	850:862	Particle size and surface properties of our optimized NE were tailored to protect the loaded insulin from acidic degradation in the hostile stomach environment and to achieve intestinal site-specific drug release as well as the improvement of oral delivery efficiency of insulin.
30537806	3	64	theme	/sodium	579:585	arg1	nanocomplex					610:620	insulin-loaded N-(2-hydroxy)-propyl-3-trimethylammonium chloride modified chitosan (HTCC)/sodium tripolyphosphate (TPP) nanocomplex	490:620	insulin-loaded N-(2-hydroxy)-propyl-3-trimethylammonium chloride modified chitosan (HTCC)/sodium tripolyphosphate (TPP) nanocomplex (NC) as a nanocore	490:639	Herein, we used insulin as a model protein drug; insulin-loaded N-(2-hydroxy)-propyl-3-trimethylammonium chloride modified chitosan (HTCC)/sodium tripolyphosphate (TPP) nanocomplex (NC) as a nanocore was further encapsulated into enteric Eudragit L100-55 material, through a two-step flash nanocomplexation (FNC) process in a reliable and scalable manner, forming our NC-in-Eudragit composite particles (NE).
30537806	3	64	theme	/sodium	579:585	arg1	NC					623:624	NC	623:624	NC	623:624	Herein, we used insulin as a model protein drug; insulin-loaded N-(2-hydroxy)-propyl-3-trimethylammonium chloride modified chitosan (HTCC)/sodium tripolyphosphate (TPP) nanocomplex (NC) as a nanocore was further encapsulated into enteric Eudragit L100-55 material, through a two-step flash nanocomplexation (FNC) process in a reliable and scalable manner, forming our NC-in-Eudragit composite particles (NE).
30537806	5	65	theme	significant	1231:1241	arg1	effect					1256:1261	a very significant hypoglycemic effect	1224:1261	a very significant hypoglycemic effect	1224:1261	In addition, the oral administration of the optimized NE to type 1 diabetic rats could induce a very significant hypoglycemic effect with a relative oral bioavailability of 13.3%.
30537806	1	66	theme	favored	126:132	arg1	mode					134:137	a more favored mode	119:137	a more favored mode of administration	119:155	Oral drug delivery is a more favored mode of administration because of its ease of administration, high patient compliance, and low healthcare costs.
30537806	1	66	theme	favored	126:132	arg1	delivery					107:114	Oral drug delivery	97:114	Oral drug delivery	97:114	Oral drug delivery is a more favored mode of administration because of its ease of administration, high patient compliance, and low healthcare costs.
30537806	6	67	theme	therapeutics	1676:1687	arg1	translation					1648:1658	clinical translation	1639:1658	clinical translation of oral protein therapeutics	1639:1687	Our results demonstrated that enteric encapsulation of nanotherapeutics using a FNC apparatus could cause drug formulations to possess better size controllability, batch-mode reproducibility, and homogeneous surface coating and then significantly enhance their oral bioavailability of insulin, indicating its great potential for clinical translation of oral protein therapeutics.
30537806	6	68	theme	nanotherapeutics	1365:1380	arg1	encapsulation					1348:1360	enteric encapsulation	1340:1360	enteric encapsulation of nanotherapeutics using a FNC apparatus	1340:1402	Our results demonstrated that enteric encapsulation of nanotherapeutics using a FNC apparatus could cause drug formulations to possess better size controllability, batch-mode reproducibility, and homogeneous surface coating and then significantly enhance their oral bioavailability of insulin, indicating its great potential for clinical translation of oral protein therapeutics.
30537806	6	69	theme	oral	1571:1574	arg1	bioavailability					1576:1590	their oral bioavailability	1565:1590	their oral bioavailability of insulin	1565:1601	Our results demonstrated that enteric encapsulation of nanotherapeutics using a FNC apparatus could cause drug formulations to possess better size controllability, batch-mode reproducibility, and homogeneous surface coating and then significantly enhance their oral bioavailability of insulin, indicating its great potential for clinical translation of oral protein therapeutics.
30537806	4	70	theme	loaded	936:941	arg1	insulin					943:949	the loaded insulin	932:949	the loaded insulin	932:949	Particle size and surface properties of our optimized NE were tailored to protect the loaded insulin from acidic degradation in the hostile stomach environment and to achieve intestinal site-specific drug release as well as the improvement of oral delivery efficiency of insulin.
30537806	3	71	from	process	754:760	arg1	manner					789:794	a reliable and scalable manner	765:794	a reliable and scalable manner	765:794	Herein, we used insulin as a model protein drug; insulin-loaded N-(2-hydroxy)-propyl-3-trimethylammonium chloride modified chitosan (HTCC)/sodium tripolyphosphate (TPP) nanocomplex (NC) as a nanocore was further encapsulated into enteric Eudragit L100-55 material, through a two-step flash nanocomplexation (FNC) process in a reliable and scalable manner, forming our NC-in-Eudragit composite particles (NE).
30537806	3	72	theme	NC-in-Eudragit	809:822	arg1	NE					845:846	NE	845:846	NE	845:846	Herein, we used insulin as a model protein drug; insulin-loaded N-(2-hydroxy)-propyl-3-trimethylammonium chloride modified chitosan (HTCC)/sodium tripolyphosphate (TPP) nanocomplex (NC) as a nanocore was further encapsulated into enteric Eudragit L100-55 material, through a two-step flash nanocomplexation (FNC) process in a reliable and scalable manner, forming our NC-in-Eudragit composite particles (NE).
30537806	3	72	theme	NC-in-Eudragit	809:822	arg1	particles					834:842	our NC-in-Eudragit composite particles	805:842	our NC-in-Eudragit composite particles (NE)	805:847	Herein, we used insulin as a model protein drug; insulin-loaded N-(2-hydroxy)-propyl-3-trimethylammonium chloride modified chitosan (HTCC)/sodium tripolyphosphate (TPP) nanocomplex (NC) as a nanocore was further encapsulated into enteric Eudragit L100-55 material, through a two-step flash nanocomplexation (FNC) process in a reliable and scalable manner, forming our NC-in-Eudragit composite particles (NE).
30537806	6	73	theme	clinical	1639:1646	arg1	translation					1648:1658	clinical translation	1639:1658	clinical translation of oral protein therapeutics	1639:1687	Our results demonstrated that enteric encapsulation of nanotherapeutics using a FNC apparatus could cause drug formulations to possess better size controllability, batch-mode reproducibility, and homogeneous surface coating and then significantly enhance their oral bioavailability of insulin, indicating its great potential for clinical translation of oral protein therapeutics.
30537806	5	74	theme	%	1307:1307	arg1	bioavailability					1284:1298	a relative oral bioavailability	1268:1298	a relative oral bioavailability of 13.3%	1268:1307	In addition, the oral administration of the optimized NE to type 1 diabetic rats could induce a very significant hypoglycemic effect with a relative oral bioavailability of 13.3%.
30537806	2	75	theme	insignificant	381:393	arg1	bioavailability					400:414	insignificant oral bioavailability	381:414	insignificant oral bioavailability of macromolecular drugs	381:438	However, no oral protein formulations are commercially available currently due to hostile gastrointestinal (GI) barriers resulting in insignificant oral bioavailability of macromolecular drugs.
30537806	1	76	theme	Oral	97:100	arg1	mode					134:137	a more favored mode	119:137	a more favored mode of administration	119:155	Oral drug delivery is a more favored mode of administration because of its ease of administration, high patient compliance, and low healthcare costs.
30537806	1	76	theme	Oral	97:100	arg1	delivery					107:114	Oral drug delivery	97:114	Oral drug delivery	97:114	Oral drug delivery is a more favored mode of administration because of its ease of administration, high patient compliance, and low healthcare costs.
30537806	4	77	theme	stomach	990:996	arg1	environment					998:1008	the hostile stomach environment	978:1008	the hostile stomach environment	978:1008	Particle size and surface properties of our optimized NE were tailored to protect the loaded insulin from acidic degradation in the hostile stomach environment and to achieve intestinal site-specific drug release as well as the improvement of oral delivery efficiency of insulin.
30537806	3	78	used	used	452:455	arg2	we					449:450	we	449:450	we	449:450	Herein, we used insulin as a model protein drug; insulin-loaded N-(2-hydroxy)-propyl-3-trimethylammonium chloride modified chitosan (HTCC)/sodium tripolyphosphate (TPP) nanocomplex (NC) as a nanocore was further encapsulated into enteric Eudragit L100-55 material, through a two-step flash nanocomplexation (FNC) process in a reliable and scalable manner, forming our NC-in-Eudragit composite particles (NE).
30537806	0	79	theme	Enteric	26:32	arg1	Systems					48:54	Enteric Encapsulation Systems	26:54	Enteric Encapsulation Systems	26:54	Scalable Manufacturing of Enteric Encapsulation Systems for Site-Specific Oral Insulin Delivery.
30537806	5	80	theme	oral	1147:1150	arg1	administration					1152:1165	the oral administration	1143:1165	the oral administration of the optimized NE to type 1 diabetic rats	1143:1209	In addition, the oral administration of the optimized NE to type 1 diabetic rats could induce a very significant hypoglycemic effect with a relative oral bioavailability of 13.3%.
30537806	5	81	theme	oral	1279:1282	arg1	bioavailability					1284:1298	a relative oral bioavailability	1268:1298	a relative oral bioavailability of 13.3%	1268:1307	In addition, the oral administration of the optimized NE to type 1 diabetic rats could induce a very significant hypoglycemic effect with a relative oral bioavailability of 13.3%.
30537806	2	82	theme	macromolecular	419:432	arg1	drugs					434:438	macromolecular drugs	419:438	macromolecular drugs	419:438	However, no oral protein formulations are commercially available currently due to hostile gastrointestinal (GI) barriers resulting in insignificant oral bioavailability of macromolecular drugs.
30537806	0	83	theme	Systems	48:54	arg1	Manufacturing					9:21	Scalable Manufacturing	0:21	Scalable Manufacturing of Enteric Encapsulation Systems for Site-Specific Oral Insulin Delivery.	0:95	Scalable Manufacturing of Enteric Encapsulation Systems for Site-Specific Oral Insulin Delivery.
30537806	1	84	theme	low	225:227	arg1	costs					240:244	low healthcare costs	225:244	low healthcare costs	225:244	Oral drug delivery is a more favored mode of administration because of its ease of administration, high patient compliance, and low healthcare costs.
30537806	0	85	theme	Site-Specific	60:72	arg1	Delivery					87:94	Site-Specific Oral Insulin Delivery	60:94	Site-Specific Oral Insulin Delivery	60:94	Scalable Manufacturing of Enteric Encapsulation Systems for Site-Specific Oral Insulin Delivery.
30537806	6	86	dep	cause	1410:1414	arg1	enhance					1557:1563	enhance	1557:1563	enhance their oral bioavailability of insulin	1557:1601	Our results demonstrated that enteric encapsulation of nanotherapeutics using a FNC apparatus could cause drug formulations to possess better size controllability, batch-mode reproducibility, and homogeneous surface coating and then significantly enhance their oral bioavailability of insulin, indicating its great potential for clinical translation of oral protein therapeutics.
30537806	2	87	theme	hostile	329:335	arg1	barriers					359:366	hostile gastrointestinal (GI) barriers	329:366	hostile gastrointestinal (GI) barriers resulting in insignificant oral bioavailability of macromolecular drugs	329:438	However, no oral protein formulations are commercially available currently due to hostile gastrointestinal (GI) barriers resulting in insignificant oral bioavailability of macromolecular drugs.
30537806	4	88	from	degradation	963:973	arg1	environment					998:1008	the hostile stomach environment	978:1008	the hostile stomach environment	978:1008	Particle size and surface properties of our optimized NE were tailored to protect the loaded insulin from acidic degradation in the hostile stomach environment and to achieve intestinal site-specific drug release as well as the improvement of oral delivery efficiency of insulin.
30537806	3	89	theme	protein	476:482	arg1	drug					484:487	a model protein drug	468:487	a model protein drug	468:487	Herein, we used insulin as a model protein drug; insulin-loaded N-(2-hydroxy)-propyl-3-trimethylammonium chloride modified chitosan (HTCC)/sodium tripolyphosphate (TPP) nanocomplex (NC) as a nanocore was further encapsulated into enteric Eudragit L100-55 material, through a two-step flash nanocomplexation (FNC) process in a reliable and scalable manner, forming our NC-in-Eudragit composite particles (NE).
30537806	3	89	theme	protein	476:482	arg1	insulin					457:463	insulin	457:463	insulin	457:463	Herein, we used insulin as a model protein drug; insulin-loaded N-(2-hydroxy)-propyl-3-trimethylammonium chloride modified chitosan (HTCC)/sodium tripolyphosphate (TPP) nanocomplex (NC) as a nanocore was further encapsulated into enteric Eudragit L100-55 material, through a two-step flash nanocomplexation (FNC) process in a reliable and scalable manner, forming our NC-in-Eudragit composite particles (NE).
30537806	3	90	theme	L100-55	688:694	arg1	material					696:703	enteric Eudragit L100-55 material	671:703	enteric Eudragit L100-55 material	671:703	Herein, we used insulin as a model protein drug; insulin-loaded N-(2-hydroxy)-propyl-3-trimethylammonium chloride modified chitosan (HTCC)/sodium tripolyphosphate (TPP) nanocomplex (NC) as a nanocore was further encapsulated into enteric Eudragit L100-55 material, through a two-step flash nanocomplexation (FNC) process in a reliable and scalable manner, forming our NC-in-Eudragit composite particles (NE).
30537806	3	91	theme	2-hydroxy	508:516	arg1	chloride					546:553	insulin-loaded N-(2-hydroxy)-propyl-3-trimethylammonium chloride	490:553	insulin-loaded N-(2-hydroxy)-propyl-3-trimethylammonium chloride modified chitosan (HTCC)/sodium tripolyphosphate (TPP) nanocomplex (NC) as a nanocore	490:639	Herein, we used insulin as a model protein drug; insulin-loaded N-(2-hydroxy)-propyl-3-trimethylammonium chloride modified chitosan (HTCC)/sodium tripolyphosphate (TPP) nanocomplex (NC) as a nanocore was further encapsulated into enteric Eudragit L100-55 material, through a two-step flash nanocomplexation (FNC) process in a reliable and scalable manner, forming our NC-in-Eudragit composite particles (NE).
30537806	6	92	theme	size	1452:1455	arg1	controllability					1457:1471	better size controllability	1445:1471	better size controllability	1445:1471	Our results demonstrated that enteric encapsulation of nanotherapeutics using a FNC apparatus could cause drug formulations to possess better size controllability, batch-mode reproducibility, and homogeneous surface coating and then significantly enhance their oral bioavailability of insulin, indicating its great potential for clinical translation of oral protein therapeutics.
30537806	1	93	theme	healthcare	229:238	arg1	costs					240:244	low healthcare costs	225:244	low healthcare costs	225:244	Oral drug delivery is a more favored mode of administration because of its ease of administration, high patient compliance, and low healthcare costs.
29734804	2	0	theme	facie	518:522	arg1	endeavor					538:545	prima facie a challenging endeavor	512:545	prima facie a challenging endeavor	512:545	Though delivery of larger as opposed to smaller and/or actively transported molecules to the brain is prima facie a challenging endeavor, we wondered whether nanoparticles could improve the therapeutic index of existing drugs for use in treating brain tumors via these vascular effects.
29734804	1	1	theme	enhanced	353:360	arg1	permeability					362:373	the enhanced permeability	349:373	the enhanced permeability of tumor-associated blood vessels	349:407	Nanoparticles provide a unique opportunity to explore the benefits of selective distribution and release of cancer therapeutics at sites of disease through varying particle sizes and compositions that exploit the enhanced permeability of tumor-associated blood vessels.
29734804	4	2	theme	tumor	1221:1225	arg1	types					1227:1231	other tumor types	1215:1231	other tumor types	1215:1231	Among a small set of nanoparticle formulations, we found evidence for nanoparticle accumulation in the brain, and one such formulation demonstrated activity in an orthotopic model of glioma, suggesting that such nanoparticles could be useful for the treatment of glioma and brain metastases of other tumor types.
29734804	1	3	theme	unique	164:169	arg1	opportunity					171:181	a unique opportunity	162:181	a unique opportunity to explore the benefits of selective distribution and release of cancer therapeutics at sites of disease through varying particle sizes and compositions that exploit the enhanced permeability of tumor-associated blood vessels	162:407	Nanoparticles provide a unique opportunity to explore the benefits of selective distribution and release of cancer therapeutics at sites of disease through varying particle sizes and compositions that exploit the enhanced permeability of tumor-associated blood vessels.
29734804	3	4	theme	cabazitaxel-carboxymethyl	757:781	arg1	polymers					805:812	cabazitaxel-carboxymethyl cellulose amphiphilic polymers	757:812	cabazitaxel-carboxymethyl cellulose amphiphilic polymers	757:812	We therefore selected a family of nanoparticles composed of cabazitaxel-carboxymethyl cellulose amphiphilic polymers to investigate the potential for delivering a brain-penetrant taxane to intracranial brain tumors in mice.
29734804	2	5	theme	prima	512:516	arg1	endeavor					538:545	prima facie a challenging endeavor	512:545	prima facie a challenging endeavor	512:545	Though delivery of larger as opposed to smaller and/or actively transported molecules to the brain is prima facie a challenging endeavor, we wondered whether nanoparticles could improve the therapeutic index of existing drugs for use in treating brain tumors via these vascular effects.
29734804	4	6	theme	brain	1195:1199	arg1	metastases					1201:1210	glioma and brain metastases	1184:1210	metastases	1201:1210	Among a small set of nanoparticle formulations, we found evidence for nanoparticle accumulation in the brain, and one such formulation demonstrated activity in an orthotopic model of glioma, suggesting that such nanoparticles could be useful for the treatment of glioma and brain metastases of other tumor types.
29734804	4	7	theme	such	1128:1131	arg1	nanoparticles					1133:1145	such nanoparticles	1128:1145	such nanoparticles	1128:1145	Among a small set of nanoparticle formulations, we found evidence for nanoparticle accumulation in the brain, and one such formulation demonstrated activity in an orthotopic model of glioma, suggesting that such nanoparticles could be useful for the treatment of glioma and brain metastases of other tumor types.
29734804	0	8	theme	Chemotherapy	104:115	arg1	Delivery					117:124	Chemotherapy Delivery	104:124	Chemotherapy Delivery	104:124	Nanoparticle Formulation Derived from Carboxymethyl Cellulose, Polyethylene Glycol, and Cabazitaxel for Chemotherapy Delivery to the Brain.
29734804	2	9	theme	drugs	630:634	arg1	index					612:616	the therapeutic index	596:616	the therapeutic index of existing drugs for use	596:642	Though delivery of larger as opposed to smaller and/or actively transported molecules to the brain is prima facie a challenging endeavor, we wondered whether nanoparticles could improve the therapeutic index of existing drugs for use in treating brain tumors via these vascular effects.
29734804	2	10	theme	vascular	679:686	arg1	effects					688:694	these vascular effects	673:694	these vascular effects	673:694	Though delivery of larger as opposed to smaller and/or actively transported molecules to the brain is prima facie a challenging endeavor, we wondered whether nanoparticles could improve the therapeutic index of existing drugs for use in treating brain tumors via these vascular effects.
29734804	3	11	theme	intracranial	886:897	arg1	tumors					905:910	intracranial brain tumors	886:910	intracranial brain tumors in mice	886:918	We therefore selected a family of nanoparticles composed of cabazitaxel-carboxymethyl cellulose amphiphilic polymers to investigate the potential for delivering a brain-penetrant taxane to intracranial brain tumors in mice.
29734804	1	12	theme	tumor-associated	378:393	arg1	vessels					401:407	tumor-associated blood vessels	378:407	tumor-associated blood vessels	378:407	Nanoparticles provide a unique opportunity to explore the benefits of selective distribution and release of cancer therapeutics at sites of disease through varying particle sizes and compositions that exploit the enhanced permeability of tumor-associated blood vessels.
29734804	2	13	theme	existing	621:628	arg1	drugs					630:634	existing drugs	621:634	existing drugs	621:634	Though delivery of larger as opposed to smaller and/or actively transported molecules to the brain is prima facie a challenging endeavor, we wondered whether nanoparticles could improve the therapeutic index of existing drugs for use in treating brain tumors via these vascular effects.
29734804	1	14	theme	disease	280:286	arg1	sites					271:275	sites	271:275	sites of disease	271:286	Nanoparticles provide a unique opportunity to explore the benefits of selective distribution and release of cancer therapeutics at sites of disease through varying particle sizes and compositions that exploit the enhanced permeability of tumor-associated blood vessels.
29734804	1	14	theme	disease	280:286	arg1	disease					280:286	disease	280:286	disease	280:286	Nanoparticles provide a unique opportunity to explore the benefits of selective distribution and release of cancer therapeutics at sites of disease through varying particle sizes and compositions that exploit the enhanced permeability of tumor-associated blood vessels.
29734804	1	15	theme	blood	395:399	arg1	vessels					401:407	tumor-associated blood vessels	378:407	tumor-associated blood vessels	378:407	Nanoparticles provide a unique opportunity to explore the benefits of selective distribution and release of cancer therapeutics at sites of disease through varying particle sizes and compositions that exploit the enhanced permeability of tumor-associated blood vessels.
29734804	4	16	theme	glioma	1184:1189	arg1	metastases					1201:1210	glioma and brain metastases	1184:1210	metastases	1201:1210	Among a small set of nanoparticle formulations, we found evidence for nanoparticle accumulation in the brain, and one such formulation demonstrated activity in an orthotopic model of glioma, suggesting that such nanoparticles could be useful for the treatment of glioma and brain metastases of other tumor types.
29734804	2	17	theme	larger	429:434	arg1	delivery					417:424	delivery	417:424	delivery of larger as opposed to smaller and/or actively transported molecules to the brain	417:507	Though delivery of larger as opposed to smaller and/or actively transported molecules to the brain is prima facie a challenging endeavor, we wondered whether nanoparticles could improve the therapeutic index of existing drugs for use in treating brain tumors via these vascular effects.
29734804	3	18	theme	brain	899:903	arg1	tumors					905:910	intracranial brain tumors	886:910	intracranial brain tumors in mice	886:918	We therefore selected a family of nanoparticles composed of cabazitaxel-carboxymethyl cellulose amphiphilic polymers to investigate the potential for delivering a brain-penetrant taxane to intracranial brain tumors in mice.
29734804	1	19	theme	vessels	401:407	arg1	permeability					362:373	the enhanced permeability	349:373	the enhanced permeability of tumor-associated blood vessels	349:407	Nanoparticles provide a unique opportunity to explore the benefits of selective distribution and release of cancer therapeutics at sites of disease through varying particle sizes and compositions that exploit the enhanced permeability of tumor-associated blood vessels.
29734804	2	20	theme	brain	656:660	arg1	tumors					662:667	brain tumors	656:667	brain tumors	656:667	Though delivery of larger as opposed to smaller and/or actively transported molecules to the brain is prima facie a challenging endeavor, we wondered whether nanoparticles could improve the therapeutic index of existing drugs for use in treating brain tumors via these vascular effects.
29734804	2	21	theme	transported	474:484	arg1	molecules					486:494	actively transported molecules	465:494	actively transported molecules	465:494	Though delivery of larger as opposed to smaller and/or actively transported molecules to the brain is prima facie a challenging endeavor, we wondered whether nanoparticles could improve the therapeutic index of existing drugs for use in treating brain tumors via these vascular effects.
29734804	4	22	theme	metastases	1201:1210	arg1	treatment					1171:1179	the treatment	1167:1179	the treatment of glioma and brain metastases of other tumor types	1167:1231	Among a small set of nanoparticle formulations, we found evidence for nanoparticle accumulation in the brain, and one such formulation demonstrated activity in an orthotopic model of glioma, suggesting that such nanoparticles could be useful for the treatment of glioma and brain metastases of other tumor types.
29734804	4	23	theme	such	1039:1042	arg1	formulation					1044:1054	one such formulation	1035:1054	one such formulation	1035:1054	Among a small set of nanoparticle formulations, we found evidence for nanoparticle accumulation in the brain, and one such formulation demonstrated activity in an orthotopic model of glioma, suggesting that such nanoparticles could be useful for the treatment of glioma and brain metastases of other tumor types.
29734804	3	24	theme	cellulose	783:791	arg1	polymers					805:812	cabazitaxel-carboxymethyl cellulose amphiphilic polymers	757:812	cabazitaxel-carboxymethyl cellulose amphiphilic polymers	757:812	We therefore selected a family of nanoparticles composed of cabazitaxel-carboxymethyl cellulose amphiphilic polymers to investigate the potential for delivering a brain-penetrant taxane to intracranial brain tumors in mice.
29734804	0	25	theme	Nanoparticle	0:11	arg1	Formulation					13:23	Nanoparticle Formulation	0:23	Nanoparticle Formulation	0:23	Nanoparticle Formulation Derived from Carboxymethyl Cellulose, Polyethylene Glycol, and Cabazitaxel for Chemotherapy Delivery to the Brain.
29734804	4	26	theme	orthotopic	1084:1093	arg1	model					1095:1099	an orthotopic model	1081:1099	an orthotopic model of glioma	1081:1109	Among a small set of nanoparticle formulations, we found evidence for nanoparticle accumulation in the brain, and one such formulation demonstrated activity in an orthotopic model of glioma, suggesting that such nanoparticles could be useful for the treatment of glioma and brain metastases of other tumor types.
29734804	4	27	theme	small	929:933	arg1	set					935:937	a small set	927:937	a small set of nanoparticle formulations	927:966	Among a small set of nanoparticle formulations, we found evidence for nanoparticle accumulation in the brain, and one such formulation demonstrated activity in an orthotopic model of glioma, suggesting that such nanoparticles could be useful for the treatment of glioma and brain metastases of other tumor types.
29734804	4	27	theme	small	929:933	arg1	formulations					955:966	nanoparticle formulations	942:966	nanoparticle formulations	942:966	Among a small set of nanoparticle formulations, we found evidence for nanoparticle accumulation in the brain, and one such formulation demonstrated activity in an orthotopic model of glioma, suggesting that such nanoparticles could be useful for the treatment of glioma and brain metastases of other tumor types.
29734804	4	28	theme	glioma	1104:1109	arg1	model					1095:1099	an orthotopic model	1081:1099	an orthotopic model of glioma	1081:1109	Among a small set of nanoparticle formulations, we found evidence for nanoparticle accumulation in the brain, and one such formulation demonstrated activity in an orthotopic model of glioma, suggesting that such nanoparticles could be useful for the treatment of glioma and brain metastases of other tumor types.
29734804	1	29	theme	varying	296:302	arg1	sizes					313:317	varying particle sizes	296:317	varying particle sizes	296:317	Nanoparticles provide a unique opportunity to explore the benefits of selective distribution and release of cancer therapeutics at sites of disease through varying particle sizes and compositions that exploit the enhanced permeability of tumor-associated blood vessels.
29734804	1	30	theme	particle	304:311	arg1	sizes					313:317	varying particle sizes	296:317	varying particle sizes	296:317	Nanoparticles provide a unique opportunity to explore the benefits of selective distribution and release of cancer therapeutics at sites of disease through varying particle sizes and compositions that exploit the enhanced permeability of tumor-associated blood vessels.
29734804	4	31	from	accumulation	1004:1015	arg1	brain					1024:1028	the brain	1020:1028	the brain	1020:1028	Among a small set of nanoparticle formulations, we found evidence for nanoparticle accumulation in the brain, and one such formulation demonstrated activity in an orthotopic model of glioma, suggesting that such nanoparticles could be useful for the treatment of glioma and brain metastases of other tumor types.
29734804	1	32	theme	selective	210:218	arg1	distribution					220:231	selective distribution	210:231	selective distribution	210:231	Nanoparticles provide a unique opportunity to explore the benefits of selective distribution and release of cancer therapeutics at sites of disease through varying particle sizes and compositions that exploit the enhanced permeability of tumor-associated blood vessels.
29734804	3	33	from	tumors	905:910	arg1	mice					915:918	mice	915:918	mice	915:918	We therefore selected a family of nanoparticles composed of cabazitaxel-carboxymethyl cellulose amphiphilic polymers to investigate the potential for delivering a brain-penetrant taxane to intracranial brain tumors in mice.
29734804	1	34	theme	distribution	220:231	arg1	benefits					198:205	the benefits	194:205	the benefits of selective distribution and release of cancer therapeutics	194:266	Nanoparticles provide a unique opportunity to explore the benefits of selective distribution and release of cancer therapeutics at sites of disease through varying particle sizes and compositions that exploit the enhanced permeability of tumor-associated blood vessels.
29734804	0	35	theme	Carboxymethyl	38:50	arg1	Cellulose					52:60	Carboxymethyl Cellulose	38:60	Carboxymethyl Cellulose	38:60	Nanoparticle Formulation Derived from Carboxymethyl Cellulose, Polyethylene Glycol, and Cabazitaxel for Chemotherapy Delivery to the Brain.
29734804	4	36	theme	nanoparticle	991:1002	arg1	accumulation					1004:1015	nanoparticle accumulation	991:1015	nanoparticle accumulation in the brain	991:1028	Among a small set of nanoparticle formulations, we found evidence for nanoparticle accumulation in the brain, and one such formulation demonstrated activity in an orthotopic model of glioma, suggesting that such nanoparticles could be useful for the treatment of glioma and brain metastases of other tumor types.
29734804	0	37	theme	Polyethylene	63:74	arg1	Glycol					76:81	Polyethylene Glycol	63:81	Polyethylene Glycol	63:81	Nanoparticle Formulation Derived from Carboxymethyl Cellulose, Polyethylene Glycol, and Cabazitaxel for Chemotherapy Delivery to the Brain.
29734804	4	38	theme	formulations	955:966	arg1	set					935:937	a small set	927:937	a small set of nanoparticle formulations	927:966	Among a small set of nanoparticle formulations, we found evidence for nanoparticle accumulation in the brain, and one such formulation demonstrated activity in an orthotopic model of glioma, suggesting that such nanoparticles could be useful for the treatment of glioma and brain metastases of other tumor types.
29734804	4	38	theme	formulations	955:966	arg1	formulations					955:966	nanoparticle formulations	942:966	nanoparticle formulations	942:966	Among a small set of nanoparticle formulations, we found evidence for nanoparticle accumulation in the brain, and one such formulation demonstrated activity in an orthotopic model of glioma, suggesting that such nanoparticles could be useful for the treatment of glioma and brain metastases of other tumor types.
29734804	4	39	theme	nanoparticle	942:953	arg1	formulations					955:966	nanoparticle formulations	942:966	nanoparticle formulations	942:966	Among a small set of nanoparticle formulations, we found evidence for nanoparticle accumulation in the brain, and one such formulation demonstrated activity in an orthotopic model of glioma, suggesting that such nanoparticles could be useful for the treatment of glioma and brain metastases of other tumor types.
29734804	2	40	theme	therapeutic	600:610	arg1	index					612:616	the therapeutic index	596:616	the therapeutic index of existing drugs for use	596:642	Though delivery of larger as opposed to smaller and/or actively transported molecules to the brain is prima facie a challenging endeavor, we wondered whether nanoparticles could improve the therapeutic index of existing drugs for use in treating brain tumors via these vascular effects.
29734804	3	41	theme	amphiphilic	793:803	arg1	polymers					805:812	cabazitaxel-carboxymethyl cellulose amphiphilic polymers	757:812	cabazitaxel-carboxymethyl cellulose amphiphilic polymers	757:812	We therefore selected a family of nanoparticles composed of cabazitaxel-carboxymethyl cellulose amphiphilic polymers to investigate the potential for delivering a brain-penetrant taxane to intracranial brain tumors in mice.
29734804	4	42	theme	types	1227:1231	arg1	metastases					1201:1210	glioma and brain metastases	1184:1210	metastases	1201:1210	Among a small set of nanoparticle formulations, we found evidence for nanoparticle accumulation in the brain, and one such formulation demonstrated activity in an orthotopic model of glioma, suggesting that such nanoparticles could be useful for the treatment of glioma and brain metastases of other tumor types.
29734804	1	43	theme	release	237:243	arg1	benefits					198:205	the benefits	194:205	the benefits of selective distribution and release of cancer therapeutics	194:266	Nanoparticles provide a unique opportunity to explore the benefits of selective distribution and release of cancer therapeutics at sites of disease through varying particle sizes and compositions that exploit the enhanced permeability of tumor-associated blood vessels.
29734804	4	44	theme	other	1215:1219	arg1	types					1227:1231	other tumor types	1215:1231	other tumor types	1215:1231	Among a small set of nanoparticle formulations, we found evidence for nanoparticle accumulation in the brain, and one such formulation demonstrated activity in an orthotopic model of glioma, suggesting that such nanoparticles could be useful for the treatment of glioma and brain metastases of other tumor types.
29734804	2	45	theme	challenging	526:536	arg1	endeavor					538:545	prima facie a challenging endeavor	512:545	prima facie a challenging endeavor	512:545	Though delivery of larger as opposed to smaller and/or actively transported molecules to the brain is prima facie a challenging endeavor, we wondered whether nanoparticles could improve the therapeutic index of existing drugs for use in treating brain tumors via these vascular effects.
29734804	2	46	dep	larger	429:434	arg1	opposed					439:445	opposed	439:445	opposed to smaller and/or actively transported molecules	439:494	Though delivery of larger as opposed to smaller and/or actively transported molecules to the brain is prima facie a challenging endeavor, we wondered whether nanoparticles could improve the therapeutic index of existing drugs for use in treating brain tumors via these vascular effects.
29734804	1	47	theme	cancer	248:253	arg1	therapeutics					255:266	cancer therapeutics	248:266	cancer therapeutics	248:266	Nanoparticles provide a unique opportunity to explore the benefits of selective distribution and release of cancer therapeutics at sites of disease through varying particle sizes and compositions that exploit the enhanced permeability of tumor-associated blood vessels.
29734804	2	48	theme	a	524:524	arg1	endeavor					538:545	prima facie a challenging endeavor	512:545	prima facie a challenging endeavor	512:545	Though delivery of larger as opposed to smaller and/or actively transported molecules to the brain is prima facie a challenging endeavor, we wondered whether nanoparticles could improve the therapeutic index of existing drugs for use in treating brain tumors via these vascular effects.
29734804	3	49	theme	brain-penetrant	860:874	arg1	taxane					876:881	a brain-penetrant taxane	858:881	a brain-penetrant taxane to intracranial brain tumors in mice	858:918	We therefore selected a family of nanoparticles composed of cabazitaxel-carboxymethyl cellulose amphiphilic polymers to investigate the potential for delivering a brain-penetrant taxane to intracranial brain tumors in mice.
29734804	3	50	theme	nanoparticles	731:743	arg1	family					721:726	a family	719:726	a family of nanoparticles composed of cabazitaxel-carboxymethyl cellulose amphiphilic polymers	719:812	We therefore selected a family of nanoparticles composed of cabazitaxel-carboxymethyl cellulose amphiphilic polymers to investigate the potential for delivering a brain-penetrant taxane to intracranial brain tumors in mice.
29734804	1	51	theme	therapeutics	255:266	arg1	benefits					198:205	the benefits	194:205	the benefits of selective distribution and release of cancer therapeutics	194:266	Nanoparticles provide a unique opportunity to explore the benefits of selective distribution and release of cancer therapeutics at sites of disease through varying particle sizes and compositions that exploit the enhanced permeability of tumor-associated blood vessels.
31915457	0	0	theme	Polysaccharides	77:91	arg1	Cells					68:72	Immunocompetent Cells	52:72	Immunocompetent Cells of Polysaccharides from Sijunzi Decoction	52:114	A Comparison of Characterization and Its Actions on Immunocompetent Cells of Polysaccharides from Sijunzi Decoction.
31915457	8	1	theme	material	1695:1702	arg1	basis					1704:1708	material basis	1695:1708	material basis	1695:1708	It will provide references for the material basis and mechanism of SJZD immunomodulation activity.
31915457	2	2	from	effect	305:310	arg1	SJZD					336:339	SJZD	336:339	SJZD	336:339	To further understand the characterization and immunomodulatory effect of polysaccharides from SJZD, the monosaccharide compositions of crude polysaccharide (SJZDP), polysaccharide fraction (S-3), and homogeneous polysaccharide (S-3-AG) from SJZD were compared by GC analysis, as well as their immunomodulatory effects on Peyer's patch cells, splenocytes, and macrophages which are related to intestinal immunity, specific immunity, and nonspecific immunity.
31915457	0	3	from	Comparison	2:11	arg1	Cells					68:72	Immunocompetent Cells	52:72	Immunocompetent Cells of Polysaccharides from Sijunzi Decoction	52:114	A Comparison of Characterization and Its Actions on Immunocompetent Cells of Polysaccharides from Sijunzi Decoction.
31915457	1	4	theme	classic	147:153	arg1	recipe					155:160	a classic recipe	145:160	a classic recipe in Traditional Chinese Medicine (TCM) with strong immune-enhancement activity	145:238	Sijunzi decoction (SJZD) is a classic recipe in Traditional Chinese Medicine (TCM) with strong immune-enhancement activity.
31915457	1	4	theme	classic	147:153	arg1	decoction					125:133	Sijunzi decoction	117:133	Sijunzi decoction (SJZD)	117:140	Sijunzi decoction (SJZD) is a classic recipe in Traditional Chinese Medicine (TCM) with strong immune-enhancement activity.
31915457	6	5	theme	macrophages	1336:1346	arg1	activity					1324:1331	the phagocytic activity	1309:1331	the phagocytic activity of macrophages	1309:1346	In addition, S-3 could stimulate the phagocytic activity of macrophages, and S-3-AG restrained the proliferation of macrophages at the concentration of 50-200 µg/mL.
31915457	5	6	from	production	1220:1229	arg1	concentrations					1260:1273	the tested concentrations	1249:1273	the tested concentrations	1249:1273	However, S-3 and S-3-AG were able to stimulate splenocytes to secret IL-4, SJZDP had no effect on IL-4 production of splenocytes in the tested concentrations.
31915457	2	7	theme	patch	571:575	arg1	cells					577:581	Peyer's patch cells	563:581	Peyer's patch cells	563:581	To further understand the characterization and immunomodulatory effect of polysaccharides from SJZD, the monosaccharide compositions of crude polysaccharide (SJZDP), polysaccharide fraction (S-3), and homogeneous polysaccharide (S-3-AG) from SJZD were compared by GC analysis, as well as their immunomodulatory effects on Peyer's patch cells, splenocytes, and macrophages which are related to intestinal immunity, specific immunity, and nonspecific immunity.
31915457	2	8	from	SJZD	336:339	arg1	polysaccharides					315:329	polysaccharides	315:329	polysaccharides from SJZD	315:339	To further understand the characterization and immunomodulatory effect of polysaccharides from SJZD, the monosaccharide compositions of crude polysaccharide (SJZDP), polysaccharide fraction (S-3), and homogeneous polysaccharide (S-3-AG) from SJZD were compared by GC analysis, as well as their immunomodulatory effects on Peyer's patch cells, splenocytes, and macrophages which are related to intestinal immunity, specific immunity, and nonspecific immunity.
31915457	2	8	from	SJZD	336:339	arg1	characterization					267:282	the characterization	263:282	the characterization	263:282	To further understand the characterization and immunomodulatory effect of polysaccharides from SJZD, the monosaccharide compositions of crude polysaccharide (SJZDP), polysaccharide fraction (S-3), and homogeneous polysaccharide (S-3-AG) from SJZD were compared by GC analysis, as well as their immunomodulatory effects on Peyer's patch cells, splenocytes, and macrophages which are related to intestinal immunity, specific immunity, and nonspecific immunity.
31915457	2	8	from	SJZD	336:339	arg1	effect					305:310	immunomodulatory effect	288:310	immunomodulatory effect of polysaccharides from SJZD	288:339	To further understand the characterization and immunomodulatory effect of polysaccharides from SJZD, the monosaccharide compositions of crude polysaccharide (SJZDP), polysaccharide fraction (S-3), and homogeneous polysaccharide (S-3-AG) from SJZD were compared by GC analysis, as well as their immunomodulatory effects on Peyer's patch cells, splenocytes, and macrophages which are related to intestinal immunity, specific immunity, and nonspecific immunity.
31915457	2	9	theme	immunomodulatory	288:303	arg1	effect					305:310	immunomodulatory effect	288:310	immunomodulatory effect of polysaccharides from SJZD	288:339	To further understand the characterization and immunomodulatory effect of polysaccharides from SJZD, the monosaccharide compositions of crude polysaccharide (SJZDP), polysaccharide fraction (S-3), and homogeneous polysaccharide (S-3-AG) from SJZD were compared by GC analysis, as well as their immunomodulatory effects on Peyer's patch cells, splenocytes, and macrophages which are related to intestinal immunity, specific immunity, and nonspecific immunity.
31915457	5	10	theme	IL-4	1215:1218	arg1	production					1220:1229	IL-4 production	1215:1229	IL-4 production of splenocytes in the tested concentrations	1215:1273	However, S-3 and S-3-AG were able to stimulate splenocytes to secret IL-4, SJZDP had no effect on IL-4 production of splenocytes in the tested concentrations.
31915457	8	11	theme	SJZD	1727:1730	arg1	activity					1749:1756	SJZD immunomodulation activity	1727:1756	SJZD immunomodulation activity	1727:1756	It will provide references for the material basis and mechanism of SJZD immunomodulation activity.
31915457	3	12	contain	contained	738:746	arg1	S-3-AG					724:729	S-3-AG	724:729	S-3-AG	724:729	The results showed that S-3-AG mainly contained Ara with a proportion of 38.9%, while Glc accounted for the largest proportion in S-3 (55.6%) and SJZDP (87.6%).
31915457	3	12	contain	contained	738:746	arg2	Ara					748:750	Ara	748:750	Ara	748:750	The results showed that S-3-AG mainly contained Ara with a proportion of 38.9%, while Glc accounted for the largest proportion in S-3 (55.6%) and SJZDP (87.6%).
31915457	2	13	theme	GC	505:506	arg1	analysis					508:515	GC analysis	505:515	GC analysis	505:515	To further understand the characterization and immunomodulatory effect of polysaccharides from SJZD, the monosaccharide compositions of crude polysaccharide (SJZDP), polysaccharide fraction (S-3), and homogeneous polysaccharide (S-3-AG) from SJZD were compared by GC analysis, as well as their immunomodulatory effects on Peyer's patch cells, splenocytes, and macrophages which are related to intestinal immunity, specific immunity, and nonspecific immunity.
31915457	1	14	theme	Sijunzi	117:123	arg1	recipe					155:160	a classic recipe	145:160	a classic recipe in Traditional Chinese Medicine (TCM) with strong immune-enhancement activity	145:238	Sijunzi decoction (SJZD) is a classic recipe in Traditional Chinese Medicine (TCM) with strong immune-enhancement activity.
31915457	1	14	theme	Sijunzi	117:123	arg1	SJZD					136:139	SJZD	136:139	SJZD	136:139	Sijunzi decoction (SJZD) is a classic recipe in Traditional Chinese Medicine (TCM) with strong immune-enhancement activity.
31915457	1	14	theme	Sijunzi	117:123	arg1	decoction					125:133	Sijunzi decoction	117:133	Sijunzi decoction (SJZD)	117:140	Sijunzi decoction (SJZD) is a classic recipe in Traditional Chinese Medicine (TCM) with strong immune-enhancement activity.
31915457	4	15	theme	patch	938:942	arg1	cells					944:948	Peyer's patch cells	930:948	Peyer's patch cells	930:948	The SJZDP, S-3, and S-3-AG all showed strong capability to stimulate Peyer's patch cells to proliferate and produce IgA and promoted the proliferation and IFN-γ production of splenocytes and increased the NO production and TNF-α production of macrophages.
31915457	7	16	contain	have	1500:1503	arg2	effects					1532:1538	different immunomodulatory effects	1505:1538	different immunomodulatory effects	1505:1538	These results suggested that SJZDP, S-3, and S-3-AG might have different immunomodulatory effects on intestinal immunity, specific immunity, and nonspecific immunity due to their different monosaccharide compositions.
31915457	7	16	contain	have	1500:1503	arg1	S-3					1478:1480	S-3	1478:1480	S-3	1478:1480	These results suggested that SJZDP, S-3, and S-3-AG might have different immunomodulatory effects on intestinal immunity, specific immunity, and nonspecific immunity due to their different monosaccharide compositions.
31915457	7	16	contain	have	1500:1503	arg1	S-3-AG					1487:1492	S-3-AG	1487:1492	S-3-AG	1487:1492	These results suggested that SJZDP, S-3, and S-3-AG might have different immunomodulatory effects on intestinal immunity, specific immunity, and nonspecific immunity due to their different monosaccharide compositions.
31915457	7	16	contain	have	1500:1503	arg1	SJZDP					1471:1475	SJZDP	1471:1475	SJZDP	1471:1475	These results suggested that SJZDP, S-3, and S-3-AG might have different immunomodulatory effects on intestinal immunity, specific immunity, and nonspecific immunity due to their different monosaccharide compositions.
31915457	4	17	theme	IFN-γ	1016:1020	arg1	production					1022:1031	IFN-γ production	1016:1031	IFN-γ production	1016:1031	The SJZDP, S-3, and S-3-AG all showed strong capability to stimulate Peyer's patch cells to proliferate and produce IgA and promoted the proliferation and IFN-γ production of splenocytes and increased the NO production and TNF-α production of macrophages.
31915457	4	18	theme	strong	899:904	arg1	capability					906:915	strong capability	899:915	strong capability to stimulate Peyer's patch cells to proliferate and produce IgA and promoted the proliferation and IFN-γ production of splenocytes	899:1046	The SJZDP, S-3, and S-3-AG all showed strong capability to stimulate Peyer's patch cells to proliferate and produce IgA and promoted the proliferation and IFN-γ production of splenocytes and increased the NO production and TNF-α production of macrophages.
31915457	2	19	theme	specific	655:662	arg1	immunity					664:671	specific immunity	655:671	specific immunity	655:671	To further understand the characterization and immunomodulatory effect of polysaccharides from SJZD, the monosaccharide compositions of crude polysaccharide (SJZDP), polysaccharide fraction (S-3), and homogeneous polysaccharide (S-3-AG) from SJZD were compared by GC analysis, as well as their immunomodulatory effects on Peyer's patch cells, splenocytes, and macrophages which are related to intestinal immunity, specific immunity, and nonspecific immunity.
31915457	3	20	theme	%	777:777	arg1	proportion					759:768	a proportion	757:768	a proportion of 38.9%	757:777	The results showed that S-3-AG mainly contained Ara with a proportion of 38.9%, while Glc accounted for the largest proportion in S-3 (55.6%) and SJZDP (87.6%).
31915457	4	21	theme	splenocytes	1036:1046	arg1	proliferation					998:1010	proliferation	998:1010	proliferation	998:1010	The SJZDP, S-3, and S-3-AG all showed strong capability to stimulate Peyer's patch cells to proliferate and produce IgA and promoted the proliferation and IFN-γ production of splenocytes and increased the NO production and TNF-α production of macrophages.
31915457	4	21	theme	splenocytes	1036:1046	arg1	production					1022:1031	IFN-γ production	1016:1031	IFN-γ production	1016:1031	The SJZDP, S-3, and S-3-AG all showed strong capability to stimulate Peyer's patch cells to proliferate and produce IgA and promoted the proliferation and IFN-γ production of splenocytes and increased the NO production and TNF-α production of macrophages.
31915457	2	22	theme	homogeneous	442:452	arg1	polysaccharide					454:467	homogeneous polysaccharide	442:467	homogeneous polysaccharide	442:467	To further understand the characterization and immunomodulatory effect of polysaccharides from SJZD, the monosaccharide compositions of crude polysaccharide (SJZDP), polysaccharide fraction (S-3), and homogeneous polysaccharide (S-3-AG) from SJZD were compared by GC analysis, as well as their immunomodulatory effects on Peyer's patch cells, splenocytes, and macrophages which are related to intestinal immunity, specific immunity, and nonspecific immunity.
31915457	7	23	theme	immunomodulatory	1515:1530	arg1	effects					1532:1538	different immunomodulatory effects	1505:1538	different immunomodulatory effects	1505:1538	These results suggested that SJZDP, S-3, and S-3-AG might have different immunomodulatory effects on intestinal immunity, specific immunity, and nonspecific immunity due to their different monosaccharide compositions.
31915457	4	24	dep	stimulate	920:928	arg1	produce					969:975	produce	969:975	produce IgA	969:979	The SJZDP, S-3, and S-3-AG all showed strong capability to stimulate Peyer's patch cells to proliferate and produce IgA and promoted the proliferation and IFN-γ production of splenocytes and increased the NO production and TNF-α production of macrophages.
31915457	4	24	dep	stimulate	920:928	arg1	promoted					985:992	promoted	985:992	promoted the proliferation and IFN-γ production of splenocytes	985:1046	The SJZDP, S-3, and S-3-AG all showed strong capability to stimulate Peyer's patch cells to proliferate and produce IgA and promoted the proliferation and IFN-γ production of splenocytes and increased the NO production and TNF-α production of macrophages.
31915457	4	24	dep	stimulate	920:928	arg1	proliferate					953:963	proliferate	953:963	proliferate	953:963	The SJZDP, S-3, and S-3-AG all showed strong capability to stimulate Peyer's patch cells to proliferate and produce IgA and promoted the proliferation and IFN-γ production of splenocytes and increased the NO production and TNF-α production of macrophages.
31915457	8	25	theme	immunomodulation	1732:1747	arg1	activity					1749:1756	SJZD immunomodulation activity	1727:1756	SJZD immunomodulation activity	1727:1756	It will provide references for the material basis and mechanism of SJZD immunomodulation activity.
31915457	0	26	from	Actions	41:47	arg1	Cells					68:72	Immunocompetent Cells	52:72	Immunocompetent Cells of Polysaccharides from Sijunzi Decoction	52:114	A Comparison of Characterization and Its Actions on Immunocompetent Cells of Polysaccharides from Sijunzi Decoction.
31915457	1	27	theme	Traditional	165:175	arg1	TCM					195:197	TCM	195:197	TCM	195:197	Sijunzi decoction (SJZD) is a classic recipe in Traditional Chinese Medicine (TCM) with strong immune-enhancement activity.
31915457	1	27	theme	Traditional	165:175	arg1	Medicine					185:192	Traditional Chinese Medicine	165:192	Traditional Chinese Medicine (TCM)	165:198	Sijunzi decoction (SJZD) is a classic recipe in Traditional Chinese Medicine (TCM) with strong immune-enhancement activity.
31915457	6	28	theme	phagocytic	1313:1322	arg1	activity					1324:1331	the phagocytic activity	1309:1331	the phagocytic activity of macrophages	1309:1346	In addition, S-3 could stimulate the phagocytic activity of macrophages, and S-3-AG restrained the proliferation of macrophages at the concentration of 50-200 µg/mL.
31915457	8	29	dep	basis	1704:1708	arg1	the					1691:1693	the	1691:1693	the	1691:1693	It will provide references for the material basis and mechanism of SJZD immunomodulation activity.
31915457	2	30	theme	polysaccharides	315:329	arg1	characterization					267:282	the characterization	263:282	the characterization	263:282	To further understand the characterization and immunomodulatory effect of polysaccharides from SJZD, the monosaccharide compositions of crude polysaccharide (SJZDP), polysaccharide fraction (S-3), and homogeneous polysaccharide (S-3-AG) from SJZD were compared by GC analysis, as well as their immunomodulatory effects on Peyer's patch cells, splenocytes, and macrophages which are related to intestinal immunity, specific immunity, and nonspecific immunity.
31915457	2	30	theme	polysaccharides	315:329	arg1	effect					305:310	immunomodulatory effect	288:310	immunomodulatory effect of polysaccharides from SJZD	288:339	To further understand the characterization and immunomodulatory effect of polysaccharides from SJZD, the monosaccharide compositions of crude polysaccharide (SJZDP), polysaccharide fraction (S-3), and homogeneous polysaccharide (S-3-AG) from SJZD were compared by GC analysis, as well as their immunomodulatory effects on Peyer's patch cells, splenocytes, and macrophages which are related to intestinal immunity, specific immunity, and nonspecific immunity.
31915457	1	31	theme	Chinese	177:183	arg1	TCM					195:197	TCM	195:197	TCM	195:197	Sijunzi decoction (SJZD) is a classic recipe in Traditional Chinese Medicine (TCM) with strong immune-enhancement activity.
31915457	1	31	theme	Chinese	177:183	arg1	Medicine					185:192	Traditional Chinese Medicine	165:192	Traditional Chinese Medicine (TCM)	165:198	Sijunzi decoction (SJZD) is a classic recipe in Traditional Chinese Medicine (TCM) with strong immune-enhancement activity.
31915457	0	32	theme	Characterization	16:31	arg1	Actions					41:47	Its Actions	37:47	Its Actions on Immunocompetent Cells of Polysaccharides from Sijunzi Decoction	37:114	A Comparison of Characterization and Its Actions on Immunocompetent Cells of Polysaccharides from Sijunzi Decoction.
31915457	0	32	theme	Characterization	16:31	arg1	Comparison					2:11	A Comparison	0:11	A Comparison of Characterization	0:31	A Comparison of Characterization and Its Actions on Immunocompetent Cells of Polysaccharides from Sijunzi Decoction.
31915457	8	33	theme	activity	1749:1756	arg1	mechanism					1714:1722	mechanism	1714:1722	mechanism	1714:1722	It will provide references for the material basis and mechanism of SJZD immunomodulation activity.
31915457	8	33	theme	activity	1749:1756	arg1	basis					1704:1708	material basis	1695:1708	material basis	1695:1708	It will provide references for the material basis and mechanism of SJZD immunomodulation activity.
31915457	2	34	theme	intestinal	634:643	arg1	immunity					645:652	intestinal immunity	634:652	intestinal immunity	634:652	To further understand the characterization and immunomodulatory effect of polysaccharides from SJZD, the monosaccharide compositions of crude polysaccharide (SJZDP), polysaccharide fraction (S-3), and homogeneous polysaccharide (S-3-AG) from SJZD were compared by GC analysis, as well as their immunomodulatory effects on Peyer's patch cells, splenocytes, and macrophages which are related to intestinal immunity, specific immunity, and nonspecific immunity.
31915457	7	35	theme	specific	1564:1571	arg1	immunity					1573:1580	specific immunity	1564:1580	specific immunity	1564:1580	These results suggested that SJZDP, S-3, and S-3-AG might have different immunomodulatory effects on intestinal immunity, specific immunity, and nonspecific immunity due to their different monosaccharide compositions.
31915457	5	36	contain	had	1198:1200	arg1	S-3					1126:1128	S-3	1126:1128	S-3	1126:1128	However, S-3 and S-3-AG were able to stimulate splenocytes to secret IL-4, SJZDP had no effect on IL-4 production of splenocytes in the tested concentrations.
31915457	5	36	contain	had	1198:1200	arg2	effect					1205:1210	no effect	1202:1210	no effect	1202:1210	However, S-3 and S-3-AG were able to stimulate splenocytes to secret IL-4, SJZDP had no effect on IL-4 production of splenocytes in the tested concentrations.
31915457	7	37	theme	different	1505:1513	arg1	effects					1532:1538	different immunomodulatory effects	1505:1538	different immunomodulatory effects	1505:1538	These results suggested that SJZDP, S-3, and S-3-AG might have different immunomodulatory effects on intestinal immunity, specific immunity, and nonspecific immunity due to their different monosaccharide compositions.
31915457	2	38	from	SJZD	483:486	arg1	compositions					361:372	the monosaccharide compositions	342:372	the monosaccharide compositions of crude polysaccharide (SJZDP), polysaccharide fraction (S-3), and homogeneous polysaccharide (S-3-AG) from SJZD	342:486	To further understand the characterization and immunomodulatory effect of polysaccharides from SJZD, the monosaccharide compositions of crude polysaccharide (SJZDP), polysaccharide fraction (S-3), and homogeneous polysaccharide (S-3-AG) from SJZD were compared by GC analysis, as well as their immunomodulatory effects on Peyer's patch cells, splenocytes, and macrophages which are related to intestinal immunity, specific immunity, and nonspecific immunity.
31915457	3	39	from	proportion	816:825	arg1	S-3					830:832	S-3	830:832	S-3 (55.6%)	830:840	The results showed that S-3-AG mainly contained Ara with a proportion of 38.9%, while Glc accounted for the largest proportion in S-3 (55.6%) and SJZDP (87.6%).
31915457	3	39	from	proportion	816:825	arg1	%					839:839	55.6%	835:839	55.6%	835:839	The results showed that S-3-AG mainly contained Ara with a proportion of 38.9%, while Glc accounted for the largest proportion in S-3 (55.6%) and SJZDP (87.6%).
31915457	3	39	from	proportion	816:825	arg1	SJZDP					846:850	SJZDP	846:850	SJZDP (87.6%)	846:858	The results showed that S-3-AG mainly contained Ara with a proportion of 38.9%, while Glc accounted for the largest proportion in S-3 (55.6%) and SJZDP (87.6%).
31915457	3	39	from	proportion	816:825	arg1	%					857:857	87.6%	853:857	87.6%	853:857	The results showed that S-3-AG mainly contained Ara with a proportion of 38.9%, while Glc accounted for the largest proportion in S-3 (55.6%) and SJZDP (87.6%).
31915457	6	40	theme	macrophages	1392:1402	arg1	proliferation					1375:1387	the proliferation	1371:1387	the proliferation of macrophages	1371:1402	In addition, S-3 could stimulate the phagocytic activity of macrophages, and S-3-AG restrained the proliferation of macrophages at the concentration of 50-200 µg/mL.
31915457	4	41	theme	macrophages	1104:1114	arg1	production					1069:1078	NO production	1066:1078	NO production	1066:1078	The SJZDP, S-3, and S-3-AG all showed strong capability to stimulate Peyer's patch cells to proliferate and produce IgA and promoted the proliferation and IFN-γ production of splenocytes and increased the NO production and TNF-α production of macrophages.
31915457	4	41	theme	macrophages	1104:1114	arg1	production					1090:1099	TNF-α production	1084:1099	TNF-α production	1084:1099	The SJZDP, S-3, and S-3-AG all showed strong capability to stimulate Peyer's patch cells to proliferate and produce IgA and promoted the proliferation and IFN-γ production of splenocytes and increased the NO production and TNF-α production of macrophages.
31915457	4	42	dep	production	1069:1078	arg1	the					1062:1064	the	1062:1064	the	1062:1064	The SJZDP, S-3, and S-3-AG all showed strong capability to stimulate Peyer's patch cells to proliferate and produce IgA and promoted the proliferation and IFN-γ production of splenocytes and increased the NO production and TNF-α production of macrophages.
31915457	2	43	theme	fraction	422:429	arg1	compositions					361:372	the monosaccharide compositions	342:372	the monosaccharide compositions of crude polysaccharide (SJZDP), polysaccharide fraction (S-3), and homogeneous polysaccharide (S-3-AG) from SJZD	342:486	To further understand the characterization and immunomodulatory effect of polysaccharides from SJZD, the monosaccharide compositions of crude polysaccharide (SJZDP), polysaccharide fraction (S-3), and homogeneous polysaccharide (S-3-AG) from SJZD were compared by GC analysis, as well as their immunomodulatory effects on Peyer's patch cells, splenocytes, and macrophages which are related to intestinal immunity, specific immunity, and nonspecific immunity.
31915457	3	44	theme	largest	808:814	arg1	proportion					816:825	the largest proportion	804:825	the largest proportion in S-3 (55.6%) and SJZDP (87.6%)	804:858	The results showed that S-3-AG mainly contained Ara with a proportion of 38.9%, while Glc accounted for the largest proportion in S-3 (55.6%) and SJZDP (87.6%).
31915457	7	45	theme	different	1621:1629	arg1	compositions					1646:1657	their different monosaccharide compositions	1615:1657	their different monosaccharide compositions	1615:1657	These results suggested that SJZDP, S-3, and S-3-AG might have different immunomodulatory effects on intestinal immunity, specific immunity, and nonspecific immunity due to their different monosaccharide compositions.
31915457	0	46	from	Cells	68:72	arg1	Decoction					106:114	Decoction	106:114	Decoction	106:114	A Comparison of Characterization and Its Actions on Immunocompetent Cells of Polysaccharides from Sijunzi Decoction.
31915457	7	47	theme	nonspecific	1587:1597	arg1	immunity					1599:1606	nonspecific immunity	1587:1606	nonspecific immunity	1587:1606	These results suggested that SJZDP, S-3, and S-3-AG might have different immunomodulatory effects on intestinal immunity, specific immunity, and nonspecific immunity due to their different monosaccharide compositions.
31915457	2	48	theme	nonspecific	678:688	arg1	immunity					690:697	nonspecific immunity	678:697	nonspecific immunity	678:697	To further understand the characterization and immunomodulatory effect of polysaccharides from SJZD, the monosaccharide compositions of crude polysaccharide (SJZDP), polysaccharide fraction (S-3), and homogeneous polysaccharide (S-3-AG) from SJZD were compared by GC analysis, as well as their immunomodulatory effects on Peyer's patch cells, splenocytes, and macrophages which are related to intestinal immunity, specific immunity, and nonspecific immunity.
31915457	2	49	theme	polysaccharide	407:420	arg1	fraction					422:429	polysaccharide fraction	407:429	polysaccharide fraction (S-3)	407:435	To further understand the characterization and immunomodulatory effect of polysaccharides from SJZD, the monosaccharide compositions of crude polysaccharide (SJZDP), polysaccharide fraction (S-3), and homogeneous polysaccharide (S-3-AG) from SJZD were compared by GC analysis, as well as their immunomodulatory effects on Peyer's patch cells, splenocytes, and macrophages which are related to intestinal immunity, specific immunity, and nonspecific immunity.
31915457	2	49	theme	polysaccharide	407:420	arg1	S-3					432:434	S-3	432:434	S-3	432:434	To further understand the characterization and immunomodulatory effect of polysaccharides from SJZD, the monosaccharide compositions of crude polysaccharide (SJZDP), polysaccharide fraction (S-3), and homogeneous polysaccharide (S-3-AG) from SJZD were compared by GC analysis, as well as their immunomodulatory effects on Peyer's patch cells, splenocytes, and macrophages which are related to intestinal immunity, specific immunity, and nonspecific immunity.
31915457	7	50	theme	intestinal	1543:1552	arg1	immunity					1554:1561	intestinal immunity	1543:1561	intestinal immunity	1543:1561	These results suggested that SJZDP, S-3, and S-3-AG might have different immunomodulatory effects on intestinal immunity, specific immunity, and nonspecific immunity due to their different monosaccharide compositions.
31915457	4	51	dep	proliferation	998:1010	arg1	the					994:996	the	994:996	the	994:996	The SJZDP, S-3, and S-3-AG all showed strong capability to stimulate Peyer's patch cells to proliferate and produce IgA and promoted the proliferation and IFN-γ production of splenocytes and increased the NO production and TNF-α production of macrophages.
31915457	2	52	theme	monosaccharide	346:359	arg1	compositions					361:372	the monosaccharide compositions	342:372	the monosaccharide compositions of crude polysaccharide (SJZDP), polysaccharide fraction (S-3), and homogeneous polysaccharide (S-3-AG) from SJZD	342:486	To further understand the characterization and immunomodulatory effect of polysaccharides from SJZD, the monosaccharide compositions of crude polysaccharide (SJZDP), polysaccharide fraction (S-3), and homogeneous polysaccharide (S-3-AG) from SJZD were compared by GC analysis, as well as their immunomodulatory effects on Peyer's patch cells, splenocytes, and macrophages which are related to intestinal immunity, specific immunity, and nonspecific immunity.
31915457	2	53	theme	polysaccharide	454:467	arg1	compositions					361:372	the monosaccharide compositions	342:372	the monosaccharide compositions of crude polysaccharide (SJZDP), polysaccharide fraction (S-3), and homogeneous polysaccharide (S-3-AG) from SJZD	342:486	To further understand the characterization and immunomodulatory effect of polysaccharides from SJZD, the monosaccharide compositions of crude polysaccharide (SJZDP), polysaccharide fraction (S-3), and homogeneous polysaccharide (S-3-AG) from SJZD were compared by GC analysis, as well as their immunomodulatory effects on Peyer's patch cells, splenocytes, and macrophages which are related to intestinal immunity, specific immunity, and nonspecific immunity.
31915457	2	54	from	characterization	267:282	arg1	SJZD					336:339	SJZD	336:339	SJZD	336:339	To further understand the characterization and immunomodulatory effect of polysaccharides from SJZD, the monosaccharide compositions of crude polysaccharide (SJZDP), polysaccharide fraction (S-3), and homogeneous polysaccharide (S-3-AG) from SJZD were compared by GC analysis, as well as their immunomodulatory effects on Peyer's patch cells, splenocytes, and macrophages which are related to intestinal immunity, specific immunity, and nonspecific immunity.
31915457	1	55	with	recipe	155:160	arg1	activity					231:238	strong immune-enhancement activity	205:238	strong immune-enhancement activity	205:238	Sijunzi decoction (SJZD) is a classic recipe in Traditional Chinese Medicine (TCM) with strong immune-enhancement activity.
31915457	0	56	from	Decoction	106:114	arg1	Polysaccharides					77:91	Polysaccharides	77:91	Polysaccharides from Sijunzi Decoction	77:114	A Comparison of Characterization and Its Actions on Immunocompetent Cells of Polysaccharides from Sijunzi Decoction.
31915457	0	56	from	Decoction	106:114	arg1	Cells					68:72	Immunocompetent Cells	52:72	Immunocompetent Cells of Polysaccharides from Sijunzi Decoction	52:114	A Comparison of Characterization and Its Actions on Immunocompetent Cells of Polysaccharides from Sijunzi Decoction.
31915457	4	57	theme	TNF-α	1084:1088	arg1	production					1090:1099	TNF-α production	1084:1099	TNF-α production	1084:1099	The SJZDP, S-3, and S-3-AG all showed strong capability to stimulate Peyer's patch cells to proliferate and produce IgA and promoted the proliferation and IFN-γ production of splenocytes and increased the NO production and TNF-α production of macrophages.
31915457	4	58	theme	NO	1066:1067	arg1	production					1069:1078	NO production	1066:1078	NO production	1066:1078	The SJZDP, S-3, and S-3-AG all showed strong capability to stimulate Peyer's patch cells to proliferate and produce IgA and promoted the proliferation and IFN-γ production of splenocytes and increased the NO production and TNF-α production of macrophages.
31915457	2	59	theme	immunomodulatory	535:550	arg1	effects					552:558	their immunomodulatory effects	529:558	their immunomodulatory effects on Peyer's patch cells, splenocytes, and macrophages which are related to intestinal immunity, specific immunity, and nonspecific immunity	529:697	To further understand the characterization and immunomodulatory effect of polysaccharides from SJZD, the monosaccharide compositions of crude polysaccharide (SJZDP), polysaccharide fraction (S-3), and homogeneous polysaccharide (S-3-AG) from SJZD were compared by GC analysis, as well as their immunomodulatory effects on Peyer's patch cells, splenocytes, and macrophages which are related to intestinal immunity, specific immunity, and nonspecific immunity.
31915457	5	60	theme	tested	1253:1258	arg1	concentrations					1260:1273	the tested concentrations	1249:1273	the tested concentrations	1249:1273	However, S-3 and S-3-AG were able to stimulate splenocytes to secret IL-4, SJZDP had no effect on IL-4 production of splenocytes in the tested concentrations.
31915457	5	61	theme	secret	1179:1184	arg1	IL-4					1186:1189	secret IL-4	1179:1189	secret IL-4	1179:1189	However, S-3 and S-3-AG were able to stimulate splenocytes to secret IL-4, SJZDP had no effect on IL-4 production of splenocytes in the tested concentrations.
31915457	1	62	theme	strong	205:210	arg1	activity					231:238	strong immune-enhancement activity	205:238	strong immune-enhancement activity	205:238	Sijunzi decoction (SJZD) is a classic recipe in Traditional Chinese Medicine (TCM) with strong immune-enhancement activity.
31915457	6	63	theme	50-200 µg/mL	1428:1439	arg1	concentration					1411:1423	the concentration	1407:1423	the concentration of 50-200 µg/mL	1407:1439	In addition, S-3 could stimulate the phagocytic activity of macrophages, and S-3-AG restrained the proliferation of macrophages at the concentration of 50-200 µg/mL.
31915457	7	64	theme	due	1608:1610	arg1	immunity					1554:1561	intestinal immunity	1543:1561	intestinal immunity	1543:1561	These results suggested that SJZDP, S-3, and S-3-AG might have different immunomodulatory effects on intestinal immunity, specific immunity, and nonspecific immunity due to their different monosaccharide compositions.
31915457	5	65	theme	splenocytes	1234:1244	arg1	production					1220:1229	IL-4 production	1215:1229	IL-4 production of splenocytes in the tested concentrations	1215:1273	However, S-3 and S-3-AG were able to stimulate splenocytes to secret IL-4, SJZDP had no effect on IL-4 production of splenocytes in the tested concentrations.
31915457	2	66	theme	polysaccharide	383:396	arg1	compositions					361:372	the monosaccharide compositions	342:372	the monosaccharide compositions of crude polysaccharide (SJZDP), polysaccharide fraction (S-3), and homogeneous polysaccharide (S-3-AG) from SJZD	342:486	To further understand the characterization and immunomodulatory effect of polysaccharides from SJZD, the monosaccharide compositions of crude polysaccharide (SJZDP), polysaccharide fraction (S-3), and homogeneous polysaccharide (S-3-AG) from SJZD were compared by GC analysis, as well as their immunomodulatory effects on Peyer's patch cells, splenocytes, and macrophages which are related to intestinal immunity, specific immunity, and nonspecific immunity.
31915457	7	67	theme	monosaccharide	1631:1644	arg1	compositions					1646:1657	their different monosaccharide compositions	1615:1657	their different monosaccharide compositions	1615:1657	These results suggested that SJZDP, S-3, and S-3-AG might have different immunomodulatory effects on intestinal immunity, specific immunity, and nonspecific immunity due to their different monosaccharide compositions.
31915457	1	68	theme	immune-enhancement	212:229	arg1	activity					231:238	strong immune-enhancement activity	205:238	strong immune-enhancement activity	205:238	Sijunzi decoction (SJZD) is a classic recipe in Traditional Chinese Medicine (TCM) with strong immune-enhancement activity.
31915457	0	69	theme	Immunocompetent	52:66	arg1	Cells					68:72	Immunocompetent Cells	52:72	Immunocompetent Cells of Polysaccharides from Sijunzi Decoction	52:114	A Comparison of Characterization and Its Actions on Immunocompetent Cells of Polysaccharides from Sijunzi Decoction.
31915457	1	70	from	recipe	155:160	arg1	TCM					195:197	TCM	195:197	TCM	195:197	Sijunzi decoction (SJZD) is a classic recipe in Traditional Chinese Medicine (TCM) with strong immune-enhancement activity.
31915457	1	70	from	recipe	155:160	arg1	Medicine					185:192	Traditional Chinese Medicine	165:192	Traditional Chinese Medicine (TCM)	165:198	Sijunzi decoction (SJZD) is a classic recipe in Traditional Chinese Medicine (TCM) with strong immune-enhancement activity.
31915457	2	71	theme	crude	377:381	arg1	S-3-AG					470:475	S-3-AG	470:475	S-3-AG	470:475	To further understand the characterization and immunomodulatory effect of polysaccharides from SJZD, the monosaccharide compositions of crude polysaccharide (SJZDP), polysaccharide fraction (S-3), and homogeneous polysaccharide (S-3-AG) from SJZD were compared by GC analysis, as well as their immunomodulatory effects on Peyer's patch cells, splenocytes, and macrophages which are related to intestinal immunity, specific immunity, and nonspecific immunity.
31915457	2	71	theme	crude	377:381	arg1	SJZDP					399:403	SJZDP	399:403	SJZDP	399:403	To further understand the characterization and immunomodulatory effect of polysaccharides from SJZD, the monosaccharide compositions of crude polysaccharide (SJZDP), polysaccharide fraction (S-3), and homogeneous polysaccharide (S-3-AG) from SJZD were compared by GC analysis, as well as their immunomodulatory effects on Peyer's patch cells, splenocytes, and macrophages which are related to intestinal immunity, specific immunity, and nonspecific immunity.
31915457	2	71	theme	crude	377:381	arg1	polysaccharide					383:396	crude polysaccharide	377:396	crude polysaccharide (SJZDP)	377:404	To further understand the characterization and immunomodulatory effect of polysaccharides from SJZD, the monosaccharide compositions of crude polysaccharide (SJZDP), polysaccharide fraction (S-3), and homogeneous polysaccharide (S-3-AG) from SJZD were compared by GC analysis, as well as their immunomodulatory effects on Peyer's patch cells, splenocytes, and macrophages which are related to intestinal immunity, specific immunity, and nonspecific immunity.
31540479	8	0	theme	peptides	1656:1663	arg1	functions					1629:1637	the immunomodulatory functions	1608:1637	the immunomodulatory functions of LL-37-derived peptides with identical positive charge and amino acid composition	1608:1721	Overall, we demonstrated that the immunomodulatory functions of LL-37-derived peptides with identical positive charge and amino acid composition are directly associated with the predicted α-helical propensity and hydrophobicity of the peptides.
31540479	6	1	theme	significant	1274:1284	arg1	%					1302:1302	>70%	1299:1302	>70%	1299:1302	Peptide RT exhibited a significant correlation (>70%) between the suppression of LPS-induced cytokine/chemokine production and peptide-induced production of the anti-inflammatory cytokine IL-1RA.
31540479	6	1	theme	significant	1274:1284	arg1	correlation					1286:1296	a significant correlation	1272:1296	a significant correlation (>70%) between the suppression of LPS-induced cytokine/chemokine production and peptide-induced production of the anti-inflammatory cytokine IL-1RA	1272:1444	Peptide RT exhibited a significant correlation (>70%) between the suppression of LPS-induced cytokine/chemokine production and peptide-induced production of the anti-inflammatory cytokine IL-1RA.
31540479	1	2	theme	independent	439:449	arg1	mechanisms					428:437	intracellular mechanisms	414:437	intracellular mechanisms independent of peptide binding to LPS	414:475	The anti-endotoxin activity of the cationic peptide LL-37 and its derivative IG-19 is attributed to electrostatic interaction of the peptides' positive charge with negatively charged bacterial lipopolysaccharides (LPS), and in part to the alteration of intracellular mechanisms independent of peptide binding to LPS.
31540479	8	3	theme	identical	1670:1678	arg1	charge					1689:1694	identical positive charge	1670:1694	identical positive charge	1670:1694	Overall, we demonstrated that the immunomodulatory functions of LL-37-derived peptides with identical positive charge and amino acid composition are directly associated with the predicted α-helical propensity and hydrophobicity of the peptides.
31540479	1	4	theme	peptide	454:460	arg1	binding					462:468	peptide binding	454:468	peptide binding to LPS	454:475	The anti-endotoxin activity of the cationic peptide LL-37 and its derivative IG-19 is attributed to electrostatic interaction of the peptides' positive charge with negatively charged bacterial lipopolysaccharides (LPS), and in part to the alteration of intracellular mechanisms independent of peptide binding to LPS.
31540479	7	5	theme	C18	1483:1485	arg1	column					1487:1492	a C18 column	1481:1492	a C18 column	1481:1492	These results indicate that RT on a C18 column can be used as a predictor for the immunomodulatory functions of cationic peptides.
31540479	4	6	theme	cytokine/chemokine	854:871	arg1	production					873:882	LPS-induced cytokine/chemokine production	842:882	LPS-induced cytokine/chemokine production	842:882	Peptide IG-19 suppressed LPS-induced cytokine/chemokine production by >90%, IG-19a and IG-19b suppressed it by 40-50%, and IG-19c and IG-19d did not suppress cytokine/chemokine production at all.
31540479	3	7	theme	net	718:720	arg1	charge					722:727	identical net charge	708:727	identical net charge (+5)	708:732	All peptides had identical net charge (+5) and amino acid composition, but different hydrophobicity and α-helical propensity.
31540479	3	7	theme	net	718:720	arg1	+5					730:731	+5	730:731	+5	730:731	All peptides had identical net charge (+5) and amino acid composition, but different hydrophobicity and α-helical propensity.
31540479	6	8	theme	Peptide	1251:1257	arg1	RT					1259:1260	Peptide RT	1251:1260	Peptide RT	1251:1260	Peptide RT exhibited a significant correlation (>70%) between the suppression of LPS-induced cytokine/chemokine production and peptide-induced production of the anti-inflammatory cytokine IL-1RA.
31540479	8	9	theme	predicted	1756:1764	arg1	propensity					1776:1785	the predicted α-helical propensity	1752:1785	the predicted α-helical propensity	1752:1785	Overall, we demonstrated that the immunomodulatory functions of LL-37-derived peptides with identical positive charge and amino acid composition are directly associated with the predicted α-helical propensity and hydrophobicity of the peptides.
31540479	7	10	from	RT	1475:1476	arg1	column					1487:1492	a C18 column	1481:1492	a C18 column	1481:1492	These results indicate that RT on a C18 column can be used as a predictor for the immunomodulatory functions of cationic peptides.
31540479	5	11	dep	In	1013:1014	arg1	silico					1016:1021	silico	1016:1021	silico	1016:1021	In silico prediction algorithms and the peptide retention time (RT) on a C18 RP HPLC column indicated a linear association between α-helical propensity and hydrophobicity with the ability of the peptides to inhibit LPS-induced responses.
31540479	3	12	theme	amino	738:742	arg1	composition					749:759	amino acid composition	738:759	amino acid composition	738:759	All peptides had identical net charge (+5) and amino acid composition, but different hydrophobicity and α-helical propensity.
31540479	0	13	theme	Predicted	107:115	arg1	Propensity					127:136	Predicted α-Helical Propensity	107:136	Predicted α-Helical Propensity	107:136	Immunomodulatory Functions of the Human Cathelicidin LL-37 (aa 13-31)-Derived Peptides are Associated with Predicted α-Helical Propensity and Hydrophobic Index.
31540479	1	14	theme	peptide	205:211	arg1	LL-37					213:217	the cationic peptide LL-37	192:217	the cationic peptide LL-37	192:217	The anti-endotoxin activity of the cationic peptide LL-37 and its derivative IG-19 is attributed to electrostatic interaction of the peptides' positive charge with negatively charged bacterial lipopolysaccharides (LPS), and in part to the alteration of intracellular mechanisms independent of peptide binding to LPS.
31540479	2	15	theme	IG-19-derived	547:559	arg1	IG-19a-d					581:588	IG-19a-d	581:588	IG-19a-d	581:588	We examined the immunomodulatory responses induced by IG-19 and four IG-19-derived scrambled peptides (IG-19a-d), in the presence and absence of LPS, in macrophages and peripheral blood-derived mononuclear cells.
31540479	2	15	theme	IG-19-derived	547:559	arg1	peptides					571:578	four IG-19-derived scrambled peptides	542:578	four IG-19-derived scrambled peptides (IG-19a-d)	542:589	We examined the immunomodulatory responses induced by IG-19 and four IG-19-derived scrambled peptides (IG-19a-d), in the presence and absence of LPS, in macrophages and peripheral blood-derived mononuclear cells.
31540479	0	16	theme	Hydrophobic	142:152	arg1	Index					154:158	Hydrophobic Index	142:158	Hydrophobic Index	142:158	Immunomodulatory Functions of the Human Cathelicidin LL-37 (aa 13-31)-Derived Peptides are Associated with Predicted α-Helical Propensity and Hydrophobic Index.
31540479	2	17	theme	blood-derived	658:670	arg1	cells					684:688	peripheral blood-derived mononuclear cells	647:688	peripheral blood-derived mononuclear cells	647:688	We examined the immunomodulatory responses induced by IG-19 and four IG-19-derived scrambled peptides (IG-19a-d), in the presence and absence of LPS, in macrophages and peripheral blood-derived mononuclear cells.
31540479	3	18	theme	α-helical	795:803	arg1	propensity					805:814	α-helical propensity	795:814	α-helical propensity	795:814	All peptides had identical net charge (+5) and amino acid composition, but different hydrophobicity and α-helical propensity.
31540479	5	19	theme	peptide	1053:1059	arg1	RT					1077:1078	RT	1077:1078	RT	1077:1078	In silico prediction algorithms and the peptide retention time (RT) on a C18 RP HPLC column indicated a linear association between α-helical propensity and hydrophobicity with the ability of the peptides to inhibit LPS-induced responses.
31540479	5	19	theme	peptide	1053:1059	arg1	time					1071:1074	the peptide retention time	1049:1074	the peptide retention time (RT) on a C18 RP HPLC column	1049:1103	In silico prediction algorithms and the peptide retention time (RT) on a C18 RP HPLC column indicated a linear association between α-helical propensity and hydrophobicity with the ability of the peptides to inhibit LPS-induced responses.
31540479	0	20	theme	aa	60:61	arg1	Peptides					78:85	the Human Cathelicidin LL-37 (aa 13-31)-Derived Peptides	30:85	the Human Cathelicidin LL-37 (aa 13-31)-Derived Peptides	30:85	Immunomodulatory Functions of the Human Cathelicidin LL-37 (aa 13-31)-Derived Peptides are Associated with Predicted α-Helical Propensity and Hydrophobic Index.
31540479	5	21	theme	HPLC	1093:1096	arg1	column					1098:1103	a C18 RP HPLC column	1084:1103	a C18 RP HPLC column	1084:1103	In silico prediction algorithms and the peptide retention time (RT) on a C18 RP HPLC column indicated a linear association between α-helical propensity and hydrophobicity with the ability of the peptides to inhibit LPS-induced responses.
31540479	4	22	theme	cytokine/chemokine	975:992	arg1	production					994:1003	cytokine/chemokine production	975:1003	cytokine/chemokine production	975:1003	Peptide IG-19 suppressed LPS-induced cytokine/chemokine production by >90%, IG-19a and IG-19b suppressed it by 40-50%, and IG-19c and IG-19d did not suppress cytokine/chemokine production at all.
31540479	6	23	theme	peptide-induced	1378:1392	arg1	production					1394:1403	peptide-induced production	1378:1403	peptide-induced production of the anti-inflammatory cytokine IL-1RA	1378:1444	Peptide RT exhibited a significant correlation (>70%) between the suppression of LPS-induced cytokine/chemokine production and peptide-induced production of the anti-inflammatory cytokine IL-1RA.
31540479	1	24	theme	intracellular	414:426	arg1	mechanisms					428:437	intracellular mechanisms	414:437	intracellular mechanisms independent of peptide binding to LPS	414:475	The anti-endotoxin activity of the cationic peptide LL-37 and its derivative IG-19 is attributed to electrostatic interaction of the peptides' positive charge with negatively charged bacterial lipopolysaccharides (LPS), and in part to the alteration of intracellular mechanisms independent of peptide binding to LPS.
31540479	0	25	theme	Peptides	78:85	arg1	Functions					17:25	Immunomodulatory Functions	0:25	Immunomodulatory Functions of the Human Cathelicidin LL-37 (aa 13-31)-Derived Peptides	0:85	Immunomodulatory Functions of the Human Cathelicidin LL-37 (aa 13-31)-Derived Peptides are Associated with Predicted α-Helical Propensity and Hydrophobic Index.
31540479	7	26	theme	peptides	1568:1575	arg1	functions					1546:1554	the immunomodulatory functions	1525:1554	the immunomodulatory functions of cationic peptides	1525:1575	These results indicate that RT on a C18 column can be used as a predictor for the immunomodulatory functions of cationic peptides.
31540479	6	27	theme	LPS-induced	1332:1342	arg1	production					1363:1372	LPS-induced cytokine/chemokine production	1332:1372	LPS-induced cytokine/chemokine production	1332:1372	Peptide RT exhibited a significant correlation (>70%) between the suppression of LPS-induced cytokine/chemokine production and peptide-induced production of the anti-inflammatory cytokine IL-1RA.
31540479	5	28	theme	C18	1086:1088	arg1	column					1098:1103	a C18 RP HPLC column	1084:1103	a C18 RP HPLC column	1084:1103	In silico prediction algorithms and the peptide retention time (RT) on a C18 RP HPLC column indicated a linear association between α-helical propensity and hydrophobicity with the ability of the peptides to inhibit LPS-induced responses.
31540479	0	29	theme	Immunomodulatory	0:15	arg1	Functions					17:25	Immunomodulatory Functions	0:25	Immunomodulatory Functions of the Human Cathelicidin LL-37 (aa 13-31)-Derived Peptides	0:85	Immunomodulatory Functions of the Human Cathelicidin LL-37 (aa 13-31)-Derived Peptides are Associated with Predicted α-Helical Propensity and Hydrophobic Index.
31540479	1	30	theme	mechanisms	428:437	arg1	alteration					400:409	the alteration	396:409	the alteration of intracellular mechanisms independent of peptide binding to LPS	396:475	The anti-endotoxin activity of the cationic peptide LL-37 and its derivative IG-19 is attributed to electrostatic interaction of the peptides' positive charge with negatively charged bacterial lipopolysaccharides (LPS), and in part to the alteration of intracellular mechanisms independent of peptide binding to LPS.
31540479	5	31	with	association	1124:1134	arg1	ability					1193:1199	the ability	1189:1199	the ability of the peptides to inhibit LPS-induced responses	1189:1248	In silico prediction algorithms and the peptide retention time (RT) on a C18 RP HPLC column indicated a linear association between α-helical propensity and hydrophobicity with the ability of the peptides to inhibit LPS-induced responses.
31540479	6	32	theme	IL-1RA	1439:1444	arg1	production					1394:1403	peptide-induced production	1378:1403	peptide-induced production of the anti-inflammatory cytokine IL-1RA	1378:1444	Peptide RT exhibited a significant correlation (>70%) between the suppression of LPS-induced cytokine/chemokine production and peptide-induced production of the anti-inflammatory cytokine IL-1RA.
31540479	6	32	theme	IL-1RA	1439:1444	arg1	suppression					1317:1327	the suppression	1313:1327	the suppression of LPS-induced cytokine/chemokine production	1313:1372	Peptide RT exhibited a significant correlation (>70%) between the suppression of LPS-induced cytokine/chemokine production and peptide-induced production of the anti-inflammatory cytokine IL-1RA.
31540479	1	33	theme	positive	304:311	arg1	charge					313:318	the peptides' positive charge	290:318	the peptides' positive charge	290:318	The anti-endotoxin activity of the cationic peptide LL-37 and its derivative IG-19 is attributed to electrostatic interaction of the peptides' positive charge with negatively charged bacterial lipopolysaccharides (LPS), and in part to the alteration of intracellular mechanisms independent of peptide binding to LPS.
31540479	0	34	theme	Human	34:38	arg1	Peptides					78:85	the Human Cathelicidin LL-37 (aa 13-31)-Derived Peptides	30:85	the Human Cathelicidin LL-37 (aa 13-31)-Derived Peptides	30:85	Immunomodulatory Functions of the Human Cathelicidin LL-37 (aa 13-31)-Derived Peptides are Associated with Predicted α-Helical Propensity and Hydrophobic Index.
31540479	5	35	theme	linear	1117:1122	arg1	association					1124:1134	a linear association	1115:1134	a linear association between α-helical propensity and hydrophobicity with the ability of the peptides to inhibit LPS-induced responses	1115:1248	In silico prediction algorithms and the peptide retention time (RT) on a C18 RP HPLC column indicated a linear association between α-helical propensity and hydrophobicity with the ability of the peptides to inhibit LPS-induced responses.
31540479	0	36	theme	LL-37	53:57	arg1	Peptides					78:85	the Human Cathelicidin LL-37 (aa 13-31)-Derived Peptides	30:85	the Human Cathelicidin LL-37 (aa 13-31)-Derived Peptides	30:85	Immunomodulatory Functions of the Human Cathelicidin LL-37 (aa 13-31)-Derived Peptides are Associated with Predicted α-Helical Propensity and Hydrophobic Index.
31540479	1	37	theme	charged	336:342	arg1	lipopolysaccharides					354:372	negatively charged bacterial lipopolysaccharides	325:372	negatively charged bacterial lipopolysaccharides (LPS)	325:378	The anti-endotoxin activity of the cationic peptide LL-37 and its derivative IG-19 is attributed to electrostatic interaction of the peptides' positive charge with negatively charged bacterial lipopolysaccharides (LPS), and in part to the alteration of intracellular mechanisms independent of peptide binding to LPS.
31540479	1	37	theme	charged	336:342	arg1	LPS					375:377	LPS	375:377	LPS	375:377	The anti-endotoxin activity of the cationic peptide LL-37 and its derivative IG-19 is attributed to electrostatic interaction of the peptides' positive charge with negatively charged bacterial lipopolysaccharides (LPS), and in part to the alteration of intracellular mechanisms independent of peptide binding to LPS.
31540479	4	38	theme	LPS-induced	842:852	arg1	production					873:882	LPS-induced cytokine/chemokine production	842:882	LPS-induced cytokine/chemokine production	842:882	Peptide IG-19 suppressed LPS-induced cytokine/chemokine production by >90%, IG-19a and IG-19b suppressed it by 40-50%, and IG-19c and IG-19d did not suppress cytokine/chemokine production at all.
31540479	8	39	theme	positive	1680:1687	arg1	charge					1689:1694	identical positive charge	1670:1694	identical positive charge	1670:1694	Overall, we demonstrated that the immunomodulatory functions of LL-37-derived peptides with identical positive charge and amino acid composition are directly associated with the predicted α-helical propensity and hydrophobicity of the peptides.
31540479	5	40	theme	peptides	1208:1215	arg1	ability					1193:1199	the ability	1189:1199	the ability of the peptides to inhibit LPS-induced responses	1189:1248	In silico prediction algorithms and the peptide retention time (RT) on a C18 RP HPLC column indicated a linear association between α-helical propensity and hydrophobicity with the ability of the peptides to inhibit LPS-induced responses.
31540479	1	41	theme	IG-19	238:242	arg1	activity					180:187	The anti-endotoxin activity	161:187	The anti-endotoxin activity of the cationic peptide LL-37 and its derivative IG-19	161:242	The anti-endotoxin activity of the cationic peptide LL-37 and its derivative IG-19 is attributed to electrostatic interaction of the peptides' positive charge with negatively charged bacterial lipopolysaccharides (LPS), and in part to the alteration of intracellular mechanisms independent of peptide binding to LPS.
31540479	3	42	theme	identical	708:716	arg1	charge					722:727	identical net charge	708:727	identical net charge (+5)	708:732	All peptides had identical net charge (+5) and amino acid composition, but different hydrophobicity and α-helical propensity.
31540479	3	42	theme	identical	708:716	arg1	+5					730:731	+5	730:731	+5	730:731	All peptides had identical net charge (+5) and amino acid composition, but different hydrophobicity and α-helical propensity.
31540479	8	43	theme	acid	1706:1709	arg1	composition					1711:1721	amino acid composition	1700:1721	amino acid composition	1700:1721	Overall, we demonstrated that the immunomodulatory functions of LL-37-derived peptides with identical positive charge and amino acid composition are directly associated with the predicted α-helical propensity and hydrophobicity of the peptides.
31540479	8	44	theme	LL-37-derived	1642:1654	arg1	peptides					1656:1663	LL-37-derived peptides	1642:1663	LL-37-derived peptides with identical positive charge and amino acid composition	1642:1721	Overall, we demonstrated that the immunomodulatory functions of LL-37-derived peptides with identical positive charge and amino acid composition are directly associated with the predicted α-helical propensity and hydrophobicity of the peptides.
31540479	1	45	theme	electrostatic	261:273	arg1	interaction					275:285	electrostatic interaction	261:285	electrostatic interaction of the peptides' positive charge with negatively charged bacterial lipopolysaccharides (LPS)	261:378	The anti-endotoxin activity of the cationic peptide LL-37 and its derivative IG-19 is attributed to electrostatic interaction of the peptides' positive charge with negatively charged bacterial lipopolysaccharides (LPS), and in part to the alteration of intracellular mechanisms independent of peptide binding to LPS.
31540479	2	46	link	IG-19-derived	547:559	arg1	IG-19a-d					581:588	IG-19a-d	581:588	IG-19a-d	581:588	We examined the immunomodulatory responses induced by IG-19 and four IG-19-derived scrambled peptides (IG-19a-d), in the presence and absence of LPS, in macrophages and peripheral blood-derived mononuclear cells.
31540479	2	46	link	IG-19-derived	547:559	arg1	peptides					571:578	four IG-19-derived scrambled peptides	542:578	four IG-19-derived scrambled peptides (IG-19a-d)	542:589	We examined the immunomodulatory responses induced by IG-19 and four IG-19-derived scrambled peptides (IG-19a-d), in the presence and absence of LPS, in macrophages and peripheral blood-derived mononuclear cells.
31540479	1	47	theme	binding	462:468	arg1	independent					439:449	independent	439:449	independent	439:449	The anti-endotoxin activity of the cationic peptide LL-37 and its derivative IG-19 is attributed to electrostatic interaction of the peptides' positive charge with negatively charged bacterial lipopolysaccharides (LPS), and in part to the alteration of intracellular mechanisms independent of peptide binding to LPS.
31540479	1	48	theme	anti-endotoxin	165:178	arg1	activity					180:187	The anti-endotoxin activity	161:187	The anti-endotoxin activity of the cationic peptide LL-37 and its derivative IG-19	161:242	The anti-endotoxin activity of the cationic peptide LL-37 and its derivative IG-19 is attributed to electrostatic interaction of the peptides' positive charge with negatively charged bacterial lipopolysaccharides (LPS), and in part to the alteration of intracellular mechanisms independent of peptide binding to LPS.
31540479	6	49	theme	anti-inflammatory	1412:1428	arg1	IL-1RA					1439:1444	the anti-inflammatory cytokine IL-1RA	1408:1444	the anti-inflammatory cytokine IL-1RA	1408:1444	Peptide RT exhibited a significant correlation (>70%) between the suppression of LPS-induced cytokine/chemokine production and peptide-induced production of the anti-inflammatory cytokine IL-1RA.
31540479	2	50	theme	LPS	623:625	arg1	presence					599:606	presence	599:606	presence	599:606	We examined the immunomodulatory responses induced by IG-19 and four IG-19-derived scrambled peptides (IG-19a-d), in the presence and absence of LPS, in macrophages and peripheral blood-derived mononuclear cells.
31540479	2	50	theme	LPS	623:625	arg1	absence					612:618	absence	612:618	absence	612:618	We examined the immunomodulatory responses induced by IG-19 and four IG-19-derived scrambled peptides (IG-19a-d), in the presence and absence of LPS, in macrophages and peripheral blood-derived mononuclear cells.
31540479	3	51	theme	acid	744:747	arg1	composition					749:759	amino acid composition	738:759	amino acid composition	738:759	All peptides had identical net charge (+5) and amino acid composition, but different hydrophobicity and α-helical propensity.
31540479	0	52	theme	α-Helical	117:125	arg1	Propensity					127:136	Predicted α-Helical Propensity	107:136	Predicted α-Helical Propensity	107:136	Immunomodulatory Functions of the Human Cathelicidin LL-37 (aa 13-31)-Derived Peptides are Associated with Predicted α-Helical Propensity and Hydrophobic Index.
31540479	8	53	with	peptides	1656:1663	arg1	composition					1711:1721	amino acid composition	1700:1721	amino acid composition	1700:1721	Overall, we demonstrated that the immunomodulatory functions of LL-37-derived peptides with identical positive charge and amino acid composition are directly associated with the predicted α-helical propensity and hydrophobicity of the peptides.
31540479	8	53	with	peptides	1656:1663	arg1	charge					1689:1694	identical positive charge	1670:1694	identical positive charge	1670:1694	Overall, we demonstrated that the immunomodulatory functions of LL-37-derived peptides with identical positive charge and amino acid composition are directly associated with the predicted α-helical propensity and hydrophobicity of the peptides.
31540479	8	54	theme	α-helical	1766:1774	arg1	propensity					1776:1785	the predicted α-helical propensity	1752:1785	the predicted α-helical propensity	1752:1785	Overall, we demonstrated that the immunomodulatory functions of LL-37-derived peptides with identical positive charge and amino acid composition are directly associated with the predicted α-helical propensity and hydrophobicity of the peptides.
31540479	1	55	theme	cationic	196:203	arg1	LL-37					213:217	the cationic peptide LL-37	192:217	the cationic peptide LL-37	192:217	The anti-endotoxin activity of the cationic peptide LL-37 and its derivative IG-19 is attributed to electrostatic interaction of the peptides' positive charge with negatively charged bacterial lipopolysaccharides (LPS), and in part to the alteration of intracellular mechanisms independent of peptide binding to LPS.
31540479	2	56	theme	scrambled	561:569	arg1	IG-19a-d					581:588	IG-19a-d	581:588	IG-19a-d	581:588	We examined the immunomodulatory responses induced by IG-19 and four IG-19-derived scrambled peptides (IG-19a-d), in the presence and absence of LPS, in macrophages and peripheral blood-derived mononuclear cells.
31540479	2	56	theme	scrambled	561:569	arg1	peptides					571:578	four IG-19-derived scrambled peptides	542:578	four IG-19-derived scrambled peptides (IG-19a-d)	542:589	We examined the immunomodulatory responses induced by IG-19 and four IG-19-derived scrambled peptides (IG-19a-d), in the presence and absence of LPS, in macrophages and peripheral blood-derived mononuclear cells.
31540479	1	57	theme	LL-37	213:217	arg1	activity					180:187	The anti-endotoxin activity	161:187	The anti-endotoxin activity of the cationic peptide LL-37 and its derivative IG-19	161:242	The anti-endotoxin activity of the cationic peptide LL-37 and its derivative IG-19 is attributed to electrostatic interaction of the peptides' positive charge with negatively charged bacterial lipopolysaccharides (LPS), and in part to the alteration of intracellular mechanisms independent of peptide binding to LPS.
31540479	3	58	theme	different	766:774	arg1	hydrophobicity					776:789	different hydrophobicity	766:789	different hydrophobicity	766:789	All peptides had identical net charge (+5) and amino acid composition, but different hydrophobicity and α-helical propensity.
31540479	2	59	dep	presence	599:606	arg1	the					595:597	the	595:597	the	595:597	We examined the immunomodulatory responses induced by IG-19 and four IG-19-derived scrambled peptides (IG-19a-d), in the presence and absence of LPS, in macrophages and peripheral blood-derived mononuclear cells.
31540479	8	60	theme	peptides	1813:1820	arg1	propensity					1776:1785	the predicted α-helical propensity	1752:1785	the predicted α-helical propensity	1752:1785	Overall, we demonstrated that the immunomodulatory functions of LL-37-derived peptides with identical positive charge and amino acid composition are directly associated with the predicted α-helical propensity and hydrophobicity of the peptides.
31540479	8	60	theme	peptides	1813:1820	arg1	hydrophobicity					1791:1804	hydrophobicity	1791:1804	hydrophobicity	1791:1804	Overall, we demonstrated that the immunomodulatory functions of LL-37-derived peptides with identical positive charge and amino acid composition are directly associated with the predicted α-helical propensity and hydrophobicity of the peptides.
31540479	5	61	from	algorithms	1034:1043	arg1	column					1098:1103	a C18 RP HPLC column	1084:1103	a C18 RP HPLC column	1084:1103	In silico prediction algorithms and the peptide retention time (RT) on a C18 RP HPLC column indicated a linear association between α-helical propensity and hydrophobicity with the ability of the peptides to inhibit LPS-induced responses.
31540479	5	62	theme	retention	1061:1069	arg1	RT					1077:1078	RT	1077:1078	RT	1077:1078	In silico prediction algorithms and the peptide retention time (RT) on a C18 RP HPLC column indicated a linear association between α-helical propensity and hydrophobicity with the ability of the peptides to inhibit LPS-induced responses.
31540479	5	62	theme	retention	1061:1069	arg1	time					1071:1074	the peptide retention time	1049:1074	the peptide retention time (RT) on a C18 RP HPLC column	1049:1103	In silico prediction algorithms and the peptide retention time (RT) on a C18 RP HPLC column indicated a linear association between α-helical propensity and hydrophobicity with the ability of the peptides to inhibit LPS-induced responses.
31540479	2	63	theme	immunomodulatory	494:509	arg1	responses					511:519	the immunomodulatory responses	490:519	the immunomodulatory responses induced by IG-19 and four IG-19-derived scrambled peptides (IG-19a-d)	490:589	We examined the immunomodulatory responses induced by IG-19 and four IG-19-derived scrambled peptides (IG-19a-d), in the presence and absence of LPS, in macrophages and peripheral blood-derived mononuclear cells.
31540479	8	64	link	LL-37-derived	1642:1654	arg1	peptides					1656:1663	LL-37-derived peptides	1642:1663	LL-37-derived peptides with identical positive charge and amino acid composition	1642:1721	Overall, we demonstrated that the immunomodulatory functions of LL-37-derived peptides with identical positive charge and amino acid composition are directly associated with the predicted α-helical propensity and hydrophobicity of the peptides.
31540479	6	65	theme	production	1363:1372	arg1	production					1394:1403	peptide-induced production	1378:1403	peptide-induced production of the anti-inflammatory cytokine IL-1RA	1378:1444	Peptide RT exhibited a significant correlation (>70%) between the suppression of LPS-induced cytokine/chemokine production and peptide-induced production of the anti-inflammatory cytokine IL-1RA.
31540479	6	65	theme	production	1363:1372	arg1	suppression					1317:1327	the suppression	1313:1327	the suppression of LPS-induced cytokine/chemokine production	1313:1372	Peptide RT exhibited a significant correlation (>70%) between the suppression of LPS-induced cytokine/chemokine production and peptide-induced production of the anti-inflammatory cytokine IL-1RA.
31540479	0	66	theme	-Derived	69:76	arg1	Peptides					78:85	the Human Cathelicidin LL-37 (aa 13-31)-Derived Peptides	30:85	the Human Cathelicidin LL-37 (aa 13-31)-Derived Peptides	30:85	Immunomodulatory Functions of the Human Cathelicidin LL-37 (aa 13-31)-Derived Peptides are Associated with Predicted α-Helical Propensity and Hydrophobic Index.
31540479	1	67	theme	bacterial	344:352	arg1	lipopolysaccharides					354:372	negatively charged bacterial lipopolysaccharides	325:372	negatively charged bacterial lipopolysaccharides (LPS)	325:378	The anti-endotoxin activity of the cationic peptide LL-37 and its derivative IG-19 is attributed to electrostatic interaction of the peptides' positive charge with negatively charged bacterial lipopolysaccharides (LPS), and in part to the alteration of intracellular mechanisms independent of peptide binding to LPS.
31540479	1	67	theme	bacterial	344:352	arg1	LPS					375:377	LPS	375:377	LPS	375:377	The anti-endotoxin activity of the cationic peptide LL-37 and its derivative IG-19 is attributed to electrostatic interaction of the peptides' positive charge with negatively charged bacterial lipopolysaccharides (LPS), and in part to the alteration of intracellular mechanisms independent of peptide binding to LPS.
31540479	2	68	theme	peripheral	647:656	arg1	cells					684:688	peripheral blood-derived mononuclear cells	647:688	peripheral blood-derived mononuclear cells	647:688	We examined the immunomodulatory responses induced by IG-19 and four IG-19-derived scrambled peptides (IG-19a-d), in the presence and absence of LPS, in macrophages and peripheral blood-derived mononuclear cells.
31540479	3	69	contain	had	704:706	arg2	charge					722:727	identical net charge	708:727	identical net charge (+5)	708:732	All peptides had identical net charge (+5) and amino acid composition, but different hydrophobicity and α-helical propensity.
31540479	3	69	contain	had	704:706	arg1	peptides					695:702	All peptides	691:702	All peptides	691:702	All peptides had identical net charge (+5) and amino acid composition, but different hydrophobicity and α-helical propensity.
31540479	3	69	contain	had	704:706	arg2	+5					730:731	+5	730:731	+5	730:731	All peptides had identical net charge (+5) and amino acid composition, but different hydrophobicity and α-helical propensity.
31540479	3	69	contain	had	704:706	arg2	hydrophobicity					776:789	different hydrophobicity	766:789	different hydrophobicity	766:789	All peptides had identical net charge (+5) and amino acid composition, but different hydrophobicity and α-helical propensity.
31540479	3	69	contain	had	704:706	arg2	propensity					805:814	α-helical propensity	795:814	α-helical propensity	795:814	All peptides had identical net charge (+5) and amino acid composition, but different hydrophobicity and α-helical propensity.
31540479	3	69	contain	had	704:706	arg2	composition					749:759	amino acid composition	738:759	amino acid composition	738:759	All peptides had identical net charge (+5) and amino acid composition, but different hydrophobicity and α-helical propensity.
31540479	7	70	theme	cationic	1559:1566	arg1	peptides					1568:1575	cationic peptides	1559:1575	cationic peptides	1559:1575	These results indicate that RT on a C18 column can be used as a predictor for the immunomodulatory functions of cationic peptides.
31540479	5	71	from	time	1071:1074	arg1	column					1098:1103	a C18 RP HPLC column	1084:1103	a C18 RP HPLC column	1084:1103	In silico prediction algorithms and the peptide retention time (RT) on a C18 RP HPLC column indicated a linear association between α-helical propensity and hydrophobicity with the ability of the peptides to inhibit LPS-induced responses.
31540479	6	72	theme	cytokine/chemokine	1344:1361	arg1	production					1363:1372	LPS-induced cytokine/chemokine production	1332:1372	LPS-induced cytokine/chemokine production	1332:1372	Peptide RT exhibited a significant correlation (>70%) between the suppression of LPS-induced cytokine/chemokine production and peptide-induced production of the anti-inflammatory cytokine IL-1RA.
31540479	5	73	theme	RP	1090:1091	arg1	column					1098:1103	a C18 RP HPLC column	1084:1103	a C18 RP HPLC column	1084:1103	In silico prediction algorithms and the peptide retention time (RT) on a C18 RP HPLC column indicated a linear association between α-helical propensity and hydrophobicity with the ability of the peptides to inhibit LPS-induced responses.
31540479	2	74	theme	mononuclear	672:682	arg1	cells					684:688	peripheral blood-derived mononuclear cells	647:688	peripheral blood-derived mononuclear cells	647:688	We examined the immunomodulatory responses induced by IG-19 and four IG-19-derived scrambled peptides (IG-19a-d), in the presence and absence of LPS, in macrophages and peripheral blood-derived mononuclear cells.
31540479	0	75	theme	Cathelicidin	40:51	arg1	Peptides					78:85	the Human Cathelicidin LL-37 (aa 13-31)-Derived Peptides	30:85	the Human Cathelicidin LL-37 (aa 13-31)-Derived Peptides	30:85	Immunomodulatory Functions of the Human Cathelicidin LL-37 (aa 13-31)-Derived Peptides are Associated with Predicted α-Helical Propensity and Hydrophobic Index.
31540479	5	76	theme	In	1013:1014	arg1	algorithms					1034:1043	In silico prediction algorithms	1013:1043	In silico prediction algorithms	1013:1043	In silico prediction algorithms and the peptide retention time (RT) on a C18 RP HPLC column indicated a linear association between α-helical propensity and hydrophobicity with the ability of the peptides to inhibit LPS-induced responses.
31540479	2	77	link	blood-derived	658:670	arg1	cells					684:688	peripheral blood-derived mononuclear cells	647:688	peripheral blood-derived mononuclear cells	647:688	We examined the immunomodulatory responses induced by IG-19 and four IG-19-derived scrambled peptides (IG-19a-d), in the presence and absence of LPS, in macrophages and peripheral blood-derived mononuclear cells.
31540479	6	78	theme	cytokine	1430:1437	arg1	IL-1RA					1439:1444	the anti-inflammatory cytokine IL-1RA	1408:1444	the anti-inflammatory cytokine IL-1RA	1408:1444	Peptide RT exhibited a significant correlation (>70%) between the suppression of LPS-induced cytokine/chemokine production and peptide-induced production of the anti-inflammatory cytokine IL-1RA.
31540479	7	79	theme	immunomodulatory	1529:1544	arg1	functions					1546:1554	the immunomodulatory functions	1525:1554	the immunomodulatory functions of cationic peptides	1525:1575	These results indicate that RT on a C18 column can be used as a predictor for the immunomodulatory functions of cationic peptides.
31540479	5	80	theme	prediction	1023:1032	arg1	algorithms					1034:1043	In silico prediction algorithms	1013:1043	In silico prediction algorithms	1013:1043	In silico prediction algorithms and the peptide retention time (RT) on a C18 RP HPLC column indicated a linear association between α-helical propensity and hydrophobicity with the ability of the peptides to inhibit LPS-induced responses.
31540479	1	81	with	interaction	275:285	arg1	lipopolysaccharides					354:372	negatively charged bacterial lipopolysaccharides	325:372	negatively charged bacterial lipopolysaccharides (LPS)	325:378	The anti-endotoxin activity of the cationic peptide LL-37 and its derivative IG-19 is attributed to electrostatic interaction of the peptides' positive charge with negatively charged bacterial lipopolysaccharides (LPS), and in part to the alteration of intracellular mechanisms independent of peptide binding to LPS.
31540479	1	81	with	interaction	275:285	arg1	LPS					375:377	LPS	375:377	LPS	375:377	The anti-endotoxin activity of the cationic peptide LL-37 and its derivative IG-19 is attributed to electrostatic interaction of the peptides' positive charge with negatively charged bacterial lipopolysaccharides (LPS), and in part to the alteration of intracellular mechanisms independent of peptide binding to LPS.
31540479	5	82	theme	α-helical	1144:1152	arg1	propensity					1154:1163	α-helical propensity	1144:1163	α-helical propensity	1144:1163	In silico prediction algorithms and the peptide retention time (RT) on a C18 RP HPLC column indicated a linear association between α-helical propensity and hydrophobicity with the ability of the peptides to inhibit LPS-induced responses.
31540479	8	83	theme	immunomodulatory	1612:1627	arg1	functions					1629:1637	the immunomodulatory functions	1608:1637	the immunomodulatory functions of LL-37-derived peptides with identical positive charge and amino acid composition	1608:1721	Overall, we demonstrated that the immunomodulatory functions of LL-37-derived peptides with identical positive charge and amino acid composition are directly associated with the predicted α-helical propensity and hydrophobicity of the peptides.
31540479	8	84	theme	amino	1700:1704	arg1	composition					1711:1721	amino acid composition	1700:1721	amino acid composition	1700:1721	Overall, we demonstrated that the immunomodulatory functions of LL-37-derived peptides with identical positive charge and amino acid composition are directly associated with the predicted α-helical propensity and hydrophobicity of the peptides.
31540479	1	85	theme	derivative	227:236	arg1	IG-19					238:242	its derivative IG-19	223:242	its derivative IG-19	223:242	The anti-endotoxin activity of the cationic peptide LL-37 and its derivative IG-19 is attributed to electrostatic interaction of the peptides' positive charge with negatively charged bacterial lipopolysaccharides (LPS), and in part to the alteration of intracellular mechanisms independent of peptide binding to LPS.
31540479	7	86	used	used	1501:1504	arg2	RT					1475:1476	RT	1475:1476	RT on a C18 column	1475:1492	These results indicate that RT on a C18 column can be used as a predictor for the immunomodulatory functions of cationic peptides.
31540479	7	86	used	used	1501:1504	arg2	column					1487:1492	a C18 column	1481:1492	a C18 column	1481:1492	These results indicate that RT on a C18 column can be used as a predictor for the immunomodulatory functions of cationic peptides.
31540479	7	86	used	used	1501:1504	arg2	predictor					1511:1519	a predictor	1509:1519	a predictor for the immunomodulatory functions of cationic peptides	1509:1575	These results indicate that RT on a C18 column can be used as a predictor for the immunomodulatory functions of cationic peptides.
31540479	5	87	theme	LPS-induced	1228:1238	arg1	responses					1240:1248	LPS-induced responses	1228:1248	LPS-induced responses	1228:1248	In silico prediction algorithms and the peptide retention time (RT) on a C18 RP HPLC column indicated a linear association between α-helical propensity and hydrophobicity with the ability of the peptides to inhibit LPS-induced responses.
31780560	5	0	theme	lactose-nonabsorber	819:837	arg1	genotypes					839:847	lactose-nonabsorber genotypes	819:847	lactose-nonabsorber genotypes	819:847	Allo-HCT patients carrying lactose-nonabsorber genotypes showed compromised clearance of postantibiotic Enterococcus domination.
31780560	3	1	from	allo-HCT	559:566	arg1	models					616:621	gnotobiotic models	604:621	gnotobiotic models	604:621	We found that Enterococcus also expands in the mouse gastrointestinal tract after allo-HCT and exacerbates disease severity in gnotobiotic models.
31780560	5	2	contain	carrying	810:817	arg2	genotypes					839:847	lactose-nonabsorber genotypes	819:847	lactose-nonabsorber genotypes	819:847	Allo-HCT patients carrying lactose-nonabsorber genotypes showed compromised clearance of postantibiotic Enterococcus domination.
31780560	5	2	contain	carrying	810:817	arg1	patients					801:808	Allo-HCT patients	792:808	Allo-HCT patients carrying lactose-nonabsorber genotypes	792:847	Allo-HCT patients carrying lactose-nonabsorber genotypes showed compromised clearance of postantibiotic Enterococcus domination.
31780560	3	3	theme	mouse	524:528	arg1	tract					547:551	the mouse gastrointestinal tract	520:551	the mouse gastrointestinal tract	520:551	We found that Enterococcus also expands in the mouse gastrointestinal tract after allo-HCT and exacerbates disease severity in gnotobiotic models.
31780560	6	4	theme	commensal	987:995	arg1	bacterium					997:1005	a commensal bacterium	985:1005	a commensal bacterium that exacerbates an intestinal and systemic inflammatory disease	985:1070	We report lactose as a common nutrient that drives expansion of a commensal bacterium that exacerbates an intestinal and systemic inflammatory disease.
31780560	2	5	theme	cell	314:317	arg1	allo-HCT					336:343	allo-HCT	336:343	allo-HCT	336:343	In patients who received allogeneic hematopoietic cell transplantation (allo-HCT), we describe a high incidence of enterococcal expansion, which was associated with graft-versus-host disease (GVHD) and mortality.
31780560	2	5	theme	cell	314:317	arg1	transplantation					319:333	allogeneic hematopoietic cell transplantation	289:333	allogeneic hematopoietic cell transplantation (allo-HCT)	289:344	In patients who received allogeneic hematopoietic cell transplantation (allo-HCT), we describe a high incidence of enterococcal expansion, which was associated with graft-versus-host disease (GVHD) and mortality.
31780560	3	6	theme	exacerbates	572:582	arg1	severity					592:599	exacerbates disease severity	572:599	exacerbates disease severity	572:599	We found that Enterococcus also expands in the mouse gastrointestinal tract after allo-HCT and exacerbates disease severity in gnotobiotic models.
31780560	4	7	theme	Enterococcus	624:635	arg1	growth					637:642	Enterococcus growth	624:642	Enterococcus growth	624:642	Enterococcus growth is dependent on the disaccharide lactose, and dietary lactose depletion attenuates Enterococcus outgrowth and reduces the severity of GVHD in mice.
31780560	4	8	theme	disaccharide	664:675	arg1	lactose					677:683	the disaccharide lactose	660:683	the disaccharide lactose	660:683	Enterococcus growth is dependent on the disaccharide lactose, and dietary lactose depletion attenuates Enterococcus outgrowth and reduces the severity of GVHD in mice.
31780560	2	9	theme	hematopoietic	300:312	arg1	allo-HCT					336:343	allo-HCT	336:343	allo-HCT	336:343	In patients who received allogeneic hematopoietic cell transplantation (allo-HCT), we describe a high incidence of enterococcal expansion, which was associated with graft-versus-host disease (GVHD) and mortality.
31780560	2	9	theme	hematopoietic	300:312	arg1	transplantation					319:333	allogeneic hematopoietic cell transplantation	289:333	allogeneic hematopoietic cell transplantation (allo-HCT)	289:344	In patients who received allogeneic hematopoietic cell transplantation (allo-HCT), we describe a high incidence of enterococcal expansion, which was associated with graft-versus-host disease (GVHD) and mortality.
31780560	4	10	theme	lactose	698:704	arg1	depletion					706:714	dietary lactose depletion	690:714	dietary lactose depletion	690:714	Enterococcus growth is dependent on the disaccharide lactose, and dietary lactose depletion attenuates Enterococcus outgrowth and reduces the severity of GVHD in mice.
31780560	5	11	theme	Allo-HCT	792:799	arg1	patients					801:808	Allo-HCT patients	792:808	Allo-HCT patients carrying lactose-nonabsorber genotypes	792:847	Allo-HCT patients carrying lactose-nonabsorber genotypes showed compromised clearance of postantibiotic Enterococcus domination.
31780560	1	12	theme	intestinal	90:99	arg1	communities					111:121	intestinal microbial communities	90:121	intestinal microbial communities	90:121	Disruption of intestinal microbial communities appears to underlie many human illnesses, but the mechanisms that promote this dysbiosis and its adverse consequences are poorly understood.
31780560	4	13	theme	dietary	690:696	arg1	depletion					706:714	dietary lactose depletion	690:714	dietary lactose depletion	690:714	Enterococcus growth is dependent on the disaccharide lactose, and dietary lactose depletion attenuates Enterococcus outgrowth and reduces the severity of GVHD in mice.
31780560	5	14	theme	postantibiotic	881:894	arg1	domination					909:918	postantibiotic Enterococcus domination	881:918	postantibiotic Enterococcus domination	881:918	Allo-HCT patients carrying lactose-nonabsorber genotypes showed compromised clearance of postantibiotic Enterococcus domination.
31780560	1	15	theme	microbial	101:109	arg1	communities					111:121	intestinal microbial communities	90:121	intestinal microbial communities	90:121	Disruption of intestinal microbial communities appears to underlie many human illnesses, but the mechanisms that promote this dysbiosis and its adverse consequences are poorly understood.
31780560	5	16	theme	Enterococcus	896:907	arg1	domination					909:918	postantibiotic Enterococcus domination	881:918	postantibiotic Enterococcus domination	881:918	Allo-HCT patients carrying lactose-nonabsorber genotypes showed compromised clearance of postantibiotic Enterococcus domination.
31780560	3	17	from	expands	509:515	arg1	tract					547:551	the mouse gastrointestinal tract	520:551	the mouse gastrointestinal tract	520:551	We found that Enterococcus also expands in the mouse gastrointestinal tract after allo-HCT and exacerbates disease severity in gnotobiotic models.
31780560	3	18	theme	gnotobiotic	604:614	arg1	models					616:621	gnotobiotic models	604:621	gnotobiotic models	604:621	We found that Enterococcus also expands in the mouse gastrointestinal tract after allo-HCT and exacerbates disease severity in gnotobiotic models.
31780560	5	19	theme	domination	909:918	arg1	clearance					868:876	compromised clearance	856:876	compromised clearance of postantibiotic Enterococcus domination	856:918	Allo-HCT patients carrying lactose-nonabsorber genotypes showed compromised clearance of postantibiotic Enterococcus domination.
31780560	3	20	theme	disease	584:590	arg1	severity					592:599	exacerbates disease severity	572:599	exacerbates disease severity	572:599	We found that Enterococcus also expands in the mouse gastrointestinal tract after allo-HCT and exacerbates disease severity in gnotobiotic models.
31780560	1	21	theme	communities	111:121	arg1	Disruption					76:85	Disruption	76:85	Disruption of intestinal microbial communities	76:121	Disruption of intestinal microbial communities appears to underlie many human illnesses, but the mechanisms that promote this dysbiosis and its adverse consequences are poorly understood.
31780560	2	22	theme	expansion	392:400	arg1	incidence					366:374	a high incidence	359:374	a high incidence of enterococcal expansion, which was associated with graft-versus-host disease (GVHD) and mortality	359:474	In patients who received allogeneic hematopoietic cell transplantation (allo-HCT), we describe a high incidence of enterococcal expansion, which was associated with graft-versus-host disease (GVHD) and mortality.
31780560	6	23	theme	inflammatory	1051:1062	arg1	disease					1064:1070	an intestinal and systemic inflammatory disease	1024:1070	an intestinal and systemic inflammatory disease	1024:1070	We report lactose as a common nutrient that drives expansion of a commensal bacterium that exacerbates an intestinal and systemic inflammatory disease.
31780560	1	24	theme	adverse	220:226	arg1	consequences					228:239	its adverse consequences	216:239	its adverse consequences	216:239	Disruption of intestinal microbial communities appears to underlie many human illnesses, but the mechanisms that promote this dysbiosis and its adverse consequences are poorly understood.
31780560	2	25	theme	graft-versus-host	429:445	arg1	GVHD					456:459	GVHD	456:459	GVHD	456:459	In patients who received allogeneic hematopoietic cell transplantation (allo-HCT), we describe a high incidence of enterococcal expansion, which was associated with graft-versus-host disease (GVHD) and mortality.
31780560	2	25	theme	graft-versus-host	429:445	arg1	disease					447:453	graft-versus-host disease	429:453	graft-versus-host disease (GVHD)	429:460	In patients who received allogeneic hematopoietic cell transplantation (allo-HCT), we describe a high incidence of enterococcal expansion, which was associated with graft-versus-host disease (GVHD) and mortality.
31780560	5	26	theme	compromised	856:866	arg1	clearance					868:876	compromised clearance	856:876	compromised clearance of postantibiotic Enterococcus domination	856:918	Allo-HCT patients carrying lactose-nonabsorber genotypes showed compromised clearance of postantibiotic Enterococcus domination.
31780560	6	27	theme	systemic	1042:1049	arg1	disease					1064:1070	an intestinal and systemic inflammatory disease	1024:1070	an intestinal and systemic inflammatory disease	1024:1070	We report lactose as a common nutrient that drives expansion of a commensal bacterium that exacerbates an intestinal and systemic inflammatory disease.
31780560	6	28	theme	common	944:949	arg1	lactose					931:937	lactose	931:937	lactose	931:937	We report lactose as a common nutrient that drives expansion of a commensal bacterium that exacerbates an intestinal and systemic inflammatory disease.
31780560	6	28	theme	common	944:949	arg1	nutrient					951:958	a common nutrient	942:958	a common nutrient that drives expansion of a commensal bacterium that exacerbates an intestinal and systemic inflammatory disease	942:1070	We report lactose as a common nutrient that drives expansion of a commensal bacterium that exacerbates an intestinal and systemic inflammatory disease.
31780560	2	29	theme	high	361:364	arg1	incidence					366:374	a high incidence	359:374	a high incidence of enterococcal expansion, which was associated with graft-versus-host disease (GVHD) and mortality	359:474	In patients who received allogeneic hematopoietic cell transplantation (allo-HCT), we describe a high incidence of enterococcal expansion, which was associated with graft-versus-host disease (GVHD) and mortality.
31780560	0	30	theme	graft-versus-host	49:65	arg1	disease					67:73	graft-versus-host disease	49:73	graft-versus-host disease	49:73	Lactose drives Enterococcus expansion to promote graft-versus-host disease.
31780560	6	31	theme	intestinal	1027:1036	arg1	disease					1064:1070	an intestinal and systemic inflammatory disease	1024:1070	an intestinal and systemic inflammatory disease	1024:1070	We report lactose as a common nutrient that drives expansion of a commensal bacterium that exacerbates an intestinal and systemic inflammatory disease.
31780560	6	32	theme	bacterium	997:1005	arg1	expansion					972:980	expansion	972:980	expansion of a commensal bacterium that exacerbates an intestinal and systemic inflammatory disease	972:1070	We report lactose as a common nutrient that drives expansion of a commensal bacterium that exacerbates an intestinal and systemic inflammatory disease.
31780560	1	33	theme	many	143:146	arg1	illnesses					154:162	many human illnesses	143:162	many human illnesses	143:162	Disruption of intestinal microbial communities appears to underlie many human illnesses, but the mechanisms that promote this dysbiosis and its adverse consequences are poorly understood.
31780560	1	34	theme	human	148:152	arg1	illnesses					154:162	many human illnesses	143:162	many human illnesses	143:162	Disruption of intestinal microbial communities appears to underlie many human illnesses, but the mechanisms that promote this dysbiosis and its adverse consequences are poorly understood.
31780560	3	35	from	severity	592:599	arg1	models					616:621	gnotobiotic models	604:621	gnotobiotic models	604:621	We found that Enterococcus also expands in the mouse gastrointestinal tract after allo-HCT and exacerbates disease severity in gnotobiotic models.
31780560	2	36	theme	allogeneic	289:298	arg1	allo-HCT					336:343	allo-HCT	336:343	allo-HCT	336:343	In patients who received allogeneic hematopoietic cell transplantation (allo-HCT), we describe a high incidence of enterococcal expansion, which was associated with graft-versus-host disease (GVHD) and mortality.
31780560	2	36	theme	allogeneic	289:298	arg1	transplantation					319:333	allogeneic hematopoietic cell transplantation	289:333	allogeneic hematopoietic cell transplantation (allo-HCT)	289:344	In patients who received allogeneic hematopoietic cell transplantation (allo-HCT), we describe a high incidence of enterococcal expansion, which was associated with graft-versus-host disease (GVHD) and mortality.
31780560	4	37	theme	Enterococcus	727:738	arg1	outgrowth					740:748	Enterococcus outgrowth	727:748	Enterococcus outgrowth	727:748	Enterococcus growth is dependent on the disaccharide lactose, and dietary lactose depletion attenuates Enterococcus outgrowth and reduces the severity of GVHD in mice.
31780560	4	38	from	severity	766:773	arg1	mice					786:789	mice	786:789	mice	786:789	Enterococcus growth is dependent on the disaccharide lactose, and dietary lactose depletion attenuates Enterococcus outgrowth and reduces the severity of GVHD in mice.
31780560	4	39	theme	GVHD	778:781	arg1	severity					766:773	the severity	762:773	the severity of GVHD in mice	762:789	Enterococcus growth is dependent on the disaccharide lactose, and dietary lactose depletion attenuates Enterococcus outgrowth and reduces the severity of GVHD in mice.
31780560	3	40	theme	gastrointestinal	530:545	arg1	tract					547:551	the mouse gastrointestinal tract	520:551	the mouse gastrointestinal tract	520:551	We found that Enterococcus also expands in the mouse gastrointestinal tract after allo-HCT and exacerbates disease severity in gnotobiotic models.
31780560	0	41	theme	Enterococcus	15:26	arg1	expansion					28:36	Enterococcus expansion	15:36	Enterococcus expansion	15:36	Lactose drives Enterococcus expansion to promote graft-versus-host disease.
31780560	2	42	theme	enterococcal	379:390	arg1	expansion					392:400	enterococcal expansion	379:400	enterococcal expansion	379:400	In patients who received allogeneic hematopoietic cell transplantation (allo-HCT), we describe a high incidence of enterococcal expansion, which was associated with graft-versus-host disease (GVHD) and mortality.
30403584	5	0	theme	KCTC	719:722	arg1	19470T					724:729	N. panacisoli KCTC 19470T	705:729	N. panacisoli KCTC 19470T	705:729	The levels of DNA-DNA relatedness between strain S-34T and N. thalensis CCTCC AB 2016296T and between strain S-34T and N. panacisoli KCTC 19470T were 50.6 and 58.8 %, respectively.
30403584	2	1	theme	strain	117:122	arg1	S-34T					124:128	A Gram-stain-positive, non-motile, rod-shaped bacterial strain S-34T	61:128	A Gram-stain-positive, non-motile, rod-shaped bacterial strain S-34T	61:128	A Gram-stain-positive, non-motile, rod-shaped bacterial strain S-34T was isolated from forest soil.
30403584	3	2	theme	strain	207:212	arg1	S-34T					214:218	strain S-34T	207:218	strain S-34T	207:218	According to 16S rRNA gene sequence analysis, strain S-34T was related to Nocardioides members and showed the highest similarities to Nocardioides thalensis NCCP-696T (97.3 %) and Nocardioides panacisoliGsoil 346T (97.0 %), Nocardioides litorisoli X-2T (96.5 %) and Nocardioides immobilis FLL521T (96.4 %).
30403584	8	3	theme	genome	1203:1208	arg1	4.53 Mb					1237:1243	4.53 Mb	1237:1243	4.53 Mb containing 52 contigs and with a DNA G+C content of 71.2 mol%	1237:1305	The genome length of strain S-34T was 4.53 Mb containing 52 contigs and with a DNA G+C content of 71.2 mol%.
30403584	8	3	theme	genome	1203:1208	arg1	length					1210:1215	The genome length	1199:1215	The genome length of strain S-34T	1199:1231	The genome length of strain S-34T was 4.53 Mb containing 52 contigs and with a DNA G+C content of 71.2 mol%.
30403584	6	4	theme	AB	837:838	arg1	2017083T					840:847	N. immobilis CCTCC AB 2017083T	818:847	N. immobilis CCTCC AB 2017083T	818:847	The genome orthoANI value between strain S-34T and N. immobilis CCTCC AB 2017083T was 82.4 %.
30403584	2	5	dep	Gram-stain-positive	63:81	arg1	rod-shaped					96:105	rod-shaped	96:105	rod-shaped	96:105	A Gram-stain-positive, non-motile, rod-shaped bacterial strain S-34T was isolated from forest soil.
30403584	2	5	dep	Gram-stain-positive	63:81	arg1	non-motile					84:93	non-motile	84:93	non-motile	84:93	A Gram-stain-positive, non-motile, rod-shaped bacterial strain S-34T was isolated from forest soil.
30403584	2	6	theme	bacterial	107:115	arg1	S-34T					124:128	A Gram-stain-positive, non-motile, rod-shaped bacterial strain S-34T	61:128	A Gram-stain-positive, non-motile, rod-shaped bacterial strain S-34T	61:128	A Gram-stain-positive, non-motile, rod-shaped bacterial strain S-34T was isolated from forest soil.
30403584	5	7	theme	relatedness	608:618	arg1	levels					590:595	The levels	586:595	The levels of DNA-DNA relatedness between strain S-34T and N. thalensis CCTCC AB 2016296T and between strain S-34T and N. panacisoli KCTC 19470T	586:729	The levels of DNA-DNA relatedness between strain S-34T and N. thalensis CCTCC AB 2016296T and between strain S-34T and N. panacisoli KCTC 19470T were 50.6 and 58.8 %, respectively.
30403584	5	7	theme	relatedness	608:618	arg1	%					750:750	50.6 and 58.8 %	736:750	50.6 and 58.8 %	736:750	The levels of DNA-DNA relatedness between strain S-34T and N. thalensis CCTCC AB 2016296T and between strain S-34T and N. panacisoli KCTC 19470T were 50.6 and 58.8 %, respectively.
30403584	5	8	theme	DNA-DNA	600:606	arg1	relatedness					608:618	DNA-DNA relatedness	600:618	DNA-DNA relatedness between strain S-34T and N. thalensis CCTCC AB 2016296T and between strain S-34T and N. panacisoli KCTC 19470T	600:729	The levels of DNA-DNA relatedness between strain S-34T and N. thalensis CCTCC AB 2016296T and between strain S-34T and N. panacisoli KCTC 19470T were 50.6 and 58.8 %, respectively.
30403584	7	9	theme	Strain	861:866	arg1	S-34T					868:872	Strain S-34T	861:872	Strain S-34T	861:872	Strain S-34T had ll-diaminopimelic acid in the cell-wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol, four unknown phospholipids and one unknown lipid as the polar lipids, meanquinone-8(H4) as the only respiratory quinone and iso-C16 : 0, C17:1ω8c, C17:1ω6c, C17 : 0 and C17 : 0 10-methyl (tbsa) as the major fatty acids.
30403584	9	10	theme	phylogenetic	1408:1419	arg1	analyses					1421:1428	phylogenetic analyses	1408:1428	phylogenetic analyses	1408:1428	Strain S-34T could be distinguished from the other Nocardioides members mainly based on the data of phylogenetic analyses, DNA-DNA hybridization, polar lipids and some biochemical differences.
30403584	1	11	theme	forest	48:53	arg1	soil					55:58	forest soil	48:58	forest soil	48:58	nov., isolated from forest soil.
30403584	7	12	dep	had	874:876	arg1	C17 					1148:1151	C17 	1148:1151	C17 	1148:1151	Strain S-34T had ll-diaminopimelic acid in the cell-wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol, four unknown phospholipids and one unknown lipid as the polar lipids, meanquinone-8(H4) as the only respiratory quinone and iso-C16 : 0, C17:1ω8c, C17:1ω6c, C17 : 0 and C17 : 0 10-methyl (tbsa) as the major fatty acids.
30403584	7	12	dep	had	874:876	arg1	 0					1112:1113	 0	1112:1113	 0	1112:1113	Strain S-34T had ll-diaminopimelic acid in the cell-wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol, four unknown phospholipids and one unknown lipid as the polar lipids, meanquinone-8(H4) as the only respiratory quinone and iso-C16 : 0, C17:1ω8c, C17:1ω6c, C17 : 0 and C17 : 0 10-methyl (tbsa) as the major fatty acids.
30403584	7	12	dep	had	874:876	arg1	C17 					1136:1139	C17 	1136:1139	C17 	1136:1139	Strain S-34T had ll-diaminopimelic acid in the cell-wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol, four unknown phospholipids and one unknown lipid as the polar lipids, meanquinone-8(H4) as the only respiratory quinone and iso-C16 : 0, C17:1ω8c, C17:1ω6c, C17 : 0 and C17 : 0 10-methyl (tbsa) as the major fatty acids.
30403584	7	12	dep	had	874:876	arg1	10-methyl					1156:1164	 0 10-methyl	1153:1164	 0 10-methyl (tbsa) as the major fatty acids	1153:1196	Strain S-34T had ll-diaminopimelic acid in the cell-wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol, four unknown phospholipids and one unknown lipid as the polar lipids, meanquinone-8(H4) as the only respiratory quinone and iso-C16 : 0, C17:1ω8c, C17:1ω6c, C17 : 0 and C17 : 0 10-methyl (tbsa) as the major fatty acids.
30403584	7	12	dep	had	874:876	arg1	 0					1141:1142	 0	1141:1142	 0	1141:1142	Strain S-34T had ll-diaminopimelic acid in the cell-wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol, four unknown phospholipids and one unknown lipid as the polar lipids, meanquinone-8(H4) as the only respiratory quinone and iso-C16 : 0, C17:1ω8c, C17:1ω6c, C17 : 0 and C17 : 0 10-methyl (tbsa) as the major fatty acids.
30403584	7	12	dep	had	874:876	arg1	tbsa					1167:1170	tbsa	1167:1170	tbsa	1167:1170	Strain S-34T had ll-diaminopimelic acid in the cell-wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol, four unknown phospholipids and one unknown lipid as the polar lipids, meanquinone-8(H4) as the only respiratory quinone and iso-C16 : 0, C17:1ω8c, C17:1ω6c, C17 : 0 and C17 : 0 10-methyl (tbsa) as the major fatty acids.
30403584	5	13	theme	strain	688:693	arg1	S-34T					695:699	strain S-34T	688:699	strain S-34T	688:699	The levels of DNA-DNA relatedness between strain S-34T and N. thalensis CCTCC AB 2016296T and between strain S-34T and N. panacisoli KCTC 19470T were 50.6 and 58.8 %, respectively.
30403584	2	14	theme	forest	148:153	arg1	soil					155:158	forest soil	148:158	forest soil	148:158	A Gram-stain-positive, non-motile, rod-shaped bacterial strain S-34T was isolated from forest soil.
30403584	5	15	theme	strain	628:633	arg1	S-34T					635:639	strain S-34T	628:639	strain S-34T	628:639	The levels of DNA-DNA relatedness between strain S-34T and N. thalensis CCTCC AB 2016296T and between strain S-34T and N. panacisoli KCTC 19470T were 50.6 and 58.8 %, respectively.
30403584	7	16	theme	ll-diaminopimelic	878:894	arg1	acid					896:899	ll-diaminopimelic acid	878:899	ll-diaminopimelic acid in the cell-wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol, four unknown phospholipids and one unknown lipid as the polar lipids, meanquinone-8(H4)	878:1065	Strain S-34T had ll-diaminopimelic acid in the cell-wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol, four unknown phospholipids and one unknown lipid as the polar lipids, meanquinone-8(H4) as the only respiratory quinone and iso-C16 : 0, C17:1ω8c, C17:1ω6c, C17 : 0 and C17 : 0 10-methyl (tbsa) as the major fatty acids.
30403584	7	16	theme	ll-diaminopimelic	878:894	arg1	quinone					1091:1097	the only respiratory quinone	1070:1097	the only respiratory quinone	1070:1097	Strain S-34T had ll-diaminopimelic acid in the cell-wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol, four unknown phospholipids and one unknown lipid as the polar lipids, meanquinone-8(H4) as the only respiratory quinone and iso-C16 : 0, C17:1ω8c, C17:1ω6c, C17 : 0 and C17 : 0 10-methyl (tbsa) as the major fatty acids.
30403584	4	17	theme	strain	547:552	arg1	S-34T					554:558	strain S-34T	547:558	strain S-34T	547:558	Phylogenetic trees showed that strain S-34T fell within the cluster containing strain S-34T and N. immobilis FLL521T.
30403584	12	18	theme	=KCTC	1669:1673	arg1	2018079T					1691:1698	=KCTC 49137T=CCTCC AB 2018079T	1669:1698	=KCTC 49137T=CCTCC AB 2018079T	1669:1698	The type strain is S-34T (=KCTC 49137T=CCTCC AB 2018079T).
30403584	12	18	theme	=KCTC	1669:1673	arg1	S-34T					1662:1666	S-34T	1662:1666	S-34T (=KCTC 49137T=CCTCC AB 2018079T)	1662:1699	The type strain is S-34T (=KCTC 49137T=CCTCC AB 2018079T).
30403584	6	19	theme	CCTCC	831:835	arg1	2017083T					840:847	N. immobilis CCTCC AB 2017083T	818:847	N. immobilis CCTCC AB 2017083T	818:847	The genome orthoANI value between strain S-34T and N. immobilis CCTCC AB 2017083T was 82.4 %.
30403584	8	20	theme	S-34T	1227:1231	arg1	4.53 Mb					1237:1243	4.53 Mb	1237:1243	4.53 Mb containing 52 contigs and with a DNA G+C content of 71.2 mol%	1237:1305	The genome length of strain S-34T was 4.53 Mb containing 52 contigs and with a DNA G+C content of 71.2 mol%.
30403584	8	20	theme	S-34T	1227:1231	arg1	length					1210:1215	The genome length	1199:1215	The genome length of strain S-34T	1199:1231	The genome length of strain S-34T was 4.53 Mb containing 52 contigs and with a DNA G+C content of 71.2 mol%.
30403584	9	21	theme	Strain	1308:1313	arg1	S-34T					1315:1319	Strain S-34T	1308:1319	Strain S-34T	1308:1319	Strain S-34T could be distinguished from the other Nocardioides members mainly based on the data of phylogenetic analyses, DNA-DNA hybridization, polar lipids and some biochemical differences.
30403584	9	22	theme	analyses	1421:1428	arg1	data					1400:1403	the data	1396:1403	the data of phylogenetic analyses, DNA-DNA hybridization, polar lipids and some biochemical differences	1396:1498	Strain S-34T could be distinguished from the other Nocardioides members mainly based on the data of phylogenetic analyses, DNA-DNA hybridization, polar lipids and some biochemical differences.
30403584	6	23	theme	immobilis	821:829	arg1	2017083T					840:847	N. immobilis CCTCC AB 2017083T	818:847	N. immobilis CCTCC AB 2017083T	818:847	The genome orthoANI value between strain S-34T and N. immobilis CCTCC AB 2017083T was 82.4 %.
30403584	3	24	theme	Nocardioides	235:246	arg1	members					248:254	Nocardioides members	235:254	Nocardioides members	235:254	According to 16S rRNA gene sequence analysis, strain S-34T was related to Nocardioides members and showed the highest similarities to Nocardioides thalensis NCCP-696T (97.3 %) and Nocardioides panacisoliGsoil 346T (97.0 %), Nocardioides litorisoli X-2T (96.5 %) and Nocardioides immobilis FLL521T (96.4 %).
30403584	3	25	theme	gene	183:186	arg1	analysis					197:204	16S rRNA gene sequence analysis	174:204	16S rRNA gene sequence analysis	174:204	According to 16S rRNA gene sequence analysis, strain S-34T was related to Nocardioides members and showed the highest similarities to Nocardioides thalensis NCCP-696T (97.3 %) and Nocardioides panacisoliGsoil 346T (97.0 %), Nocardioides litorisoli X-2T (96.5 %) and Nocardioides immobilis FLL521T (96.4 %).
30403584	8	26	theme	strain	1220:1225	arg1	S-34T					1227:1231	strain S-34T	1220:1231	strain S-34T	1220:1231	The genome length of strain S-34T was 4.53 Mb containing 52 contigs and with a DNA G+C content of 71.2 mol%.
30403584	7	27	theme	unknown	984:990	arg1	phospholipids					992:1004	four unknown phospholipids	979:1004	four unknown phospholipids	979:1004	Strain S-34T had ll-diaminopimelic acid in the cell-wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol, four unknown phospholipids and one unknown lipid as the polar lipids, meanquinone-8(H4) as the only respiratory quinone and iso-C16 : 0, C17:1ω8c, C17:1ω6c, C17 : 0 and C17 : 0 10-methyl (tbsa) as the major fatty acids.
30403584	7	27	theme	unknown	984:990	arg1	peptidoglycan					918:930	the cell-wall peptidoglycan	904:930	the cell-wall peptidoglycan	904:930	Strain S-34T had ll-diaminopimelic acid in the cell-wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol, four unknown phospholipids and one unknown lipid as the polar lipids, meanquinone-8(H4) as the only respiratory quinone and iso-C16 : 0, C17:1ω8c, C17:1ω6c, C17 : 0 and C17 : 0 10-methyl (tbsa) as the major fatty acids.
30403584	0	28	theme	silvaticus	13:22	arg1	sp					24:25	Nocardioides silvaticus sp	0:25	Nocardioides silvaticus sp.	0:26	Nocardioides silvaticus sp.
30403584	6	29	theme	N.	818:819	arg1	2017083T					840:847	N. immobilis CCTCC AB 2017083T	818:847	N. immobilis CCTCC AB 2017083T	818:847	The genome orthoANI value between strain S-34T and N. immobilis CCTCC AB 2017083T was 82.4 %.
30403584	7	30	theme	polar	1035:1039	arg1	lipids					1041:1046	the polar lipids	1031:1046	the polar lipids	1031:1046	Strain S-34T had ll-diaminopimelic acid in the cell-wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol, four unknown phospholipids and one unknown lipid as the polar lipids, meanquinone-8(H4) as the only respiratory quinone and iso-C16 : 0, C17:1ω8c, C17:1ω6c, C17 : 0 and C17 : 0 10-methyl (tbsa) as the major fatty acids.
30403584	3	31	theme	sequence	188:195	arg1	analysis					197:204	16S rRNA gene sequence analysis	174:204	16S rRNA gene sequence analysis	174:204	According to 16S rRNA gene sequence analysis, strain S-34T was related to Nocardioides members and showed the highest similarities to Nocardioides thalensis NCCP-696T (97.3 %) and Nocardioides panacisoliGsoil 346T (97.0 %), Nocardioides litorisoli X-2T (96.5 %) and Nocardioides immobilis FLL521T (96.4 %).
30403584	10	32	theme	strain	1512:1517	arg1	S-34T					1519:1523	strain S-34T	1512:1523	strain S-34T	1512:1523	Therefore, strain S-34T represents a novel species of the genus Nocardioides, for which the name Nocardioidessilvaticus sp.
30403584	8	33	dep	4.53 Mb	1237:1243	arg1	G+C content					1282:1292	a DNA G+C content	1276:1292	a DNA G+C content of 71.2 mol%	1276:1305	The genome length of strain S-34T was 4.53 Mb containing 52 contigs and with a DNA G+C content of 71.2 mol%.
30403584	8	33	dep	4.53 Mb	1237:1243	arg1	containing					1245:1254	containing	1245:1254	containing 52 contigs	1245:1265	The genome length of strain S-34T was 4.53 Mb containing 52 contigs and with a DNA G+C content of 71.2 mol%.
30403584	0	34	theme	Nocardioides	0:11	arg1	sp					24:25	Nocardioides silvaticus sp	0:25	Nocardioides silvaticus sp.	0:26	Nocardioides silvaticus sp.
30403584	9	35	theme	differences	1488:1498	arg1	data					1400:1403	the data	1396:1403	the data of phylogenetic analyses, DNA-DNA hybridization, polar lipids and some biochemical differences	1396:1498	Strain S-34T could be distinguished from the other Nocardioides members mainly based on the data of phylogenetic analyses, DNA-DNA hybridization, polar lipids and some biochemical differences.
30403584	3	36	theme	16S	174:176	arg1	analysis					197:204	16S rRNA gene sequence analysis	174:204	16S rRNA gene sequence analysis	174:204	According to 16S rRNA gene sequence analysis, strain S-34T was related to Nocardioides members and showed the highest similarities to Nocardioides thalensis NCCP-696T (97.3 %) and Nocardioides panacisoliGsoil 346T (97.0 %), Nocardioides litorisoli X-2T (96.5 %) and Nocardioides immobilis FLL521T (96.4 %).
30403584	9	37	theme	DNA-DNA	1431:1437	arg1	hybridization					1439:1451	DNA-DNA hybridization	1431:1451	DNA-DNA hybridization	1431:1451	Strain S-34T could be distinguished from the other Nocardioides members mainly based on the data of phylogenetic analyses, DNA-DNA hybridization, polar lipids and some biochemical differences.
30403584	7	38	contain	had	874:876	arg1	S-34T					868:872	Strain S-34T	861:872	Strain S-34T	861:872	Strain S-34T had ll-diaminopimelic acid in the cell-wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol, four unknown phospholipids and one unknown lipid as the polar lipids, meanquinone-8(H4) as the only respiratory quinone and iso-C16 : 0, C17:1ω8c, C17:1ω6c, C17 : 0 and C17 : 0 10-methyl (tbsa) as the major fatty acids.
30403584	7	38	contain	had	874:876	arg2	acid					896:899	ll-diaminopimelic acid	878:899	ll-diaminopimelic acid in the cell-wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol, four unknown phospholipids and one unknown lipid as the polar lipids, meanquinone-8(H4)	878:1065	Strain S-34T had ll-diaminopimelic acid in the cell-wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol, four unknown phospholipids and one unknown lipid as the polar lipids, meanquinone-8(H4) as the only respiratory quinone and iso-C16 : 0, C17:1ω8c, C17:1ω6c, C17 : 0 and C17 : 0 10-methyl (tbsa) as the major fatty acids.
30403584	7	38	contain	had	874:876	arg2	quinone					1091:1097	the only respiratory quinone	1070:1097	the only respiratory quinone	1070:1097	Strain S-34T had ll-diaminopimelic acid in the cell-wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol, four unknown phospholipids and one unknown lipid as the polar lipids, meanquinone-8(H4) as the only respiratory quinone and iso-C16 : 0, C17:1ω8c, C17:1ω6c, C17 : 0 and C17 : 0 10-methyl (tbsa) as the major fatty acids.
30403584	5	39	theme	CCTCC	658:662	arg1	2016296T					667:674	N. thalensis CCTCC AB 2016296T	645:674	N. thalensis CCTCC AB 2016296T	645:674	The levels of DNA-DNA relatedness between strain S-34T and N. thalensis CCTCC AB 2016296T and between strain S-34T and N. panacisoli KCTC 19470T were 50.6 and 58.8 %, respectively.
30403584	3	40	theme	rRNA	178:181	arg1	analysis					197:204	16S rRNA gene sequence analysis	174:204	16S rRNA gene sequence analysis	174:204	According to 16S rRNA gene sequence analysis, strain S-34T was related to Nocardioides members and showed the highest similarities to Nocardioides thalensis NCCP-696T (97.3 %) and Nocardioides panacisoliGsoil 346T (97.0 %), Nocardioides litorisoli X-2T (96.5 %) and Nocardioides immobilis FLL521T (96.4 %).
30403584	9	41	theme	hybridization	1439:1451	arg1	data					1400:1403	the data	1396:1403	the data of phylogenetic analyses, DNA-DNA hybridization, polar lipids and some biochemical differences	1396:1498	Strain S-34T could be distinguished from the other Nocardioides members mainly based on the data of phylogenetic analyses, DNA-DNA hybridization, polar lipids and some biochemical differences.
30403584	7	42	theme	major	1180:1184	arg1	acids					1192:1196	the major fatty acids	1176:1196	the major fatty acids	1176:1196	Strain S-34T had ll-diaminopimelic acid in the cell-wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol, four unknown phospholipids and one unknown lipid as the polar lipids, meanquinone-8(H4) as the only respiratory quinone and iso-C16 : 0, C17:1ω8c, C17:1ω6c, C17 : 0 and C17 : 0 10-methyl (tbsa) as the major fatty acids.
30403584	5	43	theme	AB	664:665	arg1	2016296T					667:674	N. thalensis CCTCC AB 2016296T	645:674	N. thalensis CCTCC AB 2016296T	645:674	The levels of DNA-DNA relatedness between strain S-34T and N. thalensis CCTCC AB 2016296T and between strain S-34T and N. panacisoli KCTC 19470T were 50.6 and 58.8 %, respectively.
30403584	7	44	theme	fatty	1186:1190	arg1	acids					1192:1196	the major fatty acids	1176:1196	the major fatty acids	1176:1196	Strain S-34T had ll-diaminopimelic acid in the cell-wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol, four unknown phospholipids and one unknown lipid as the polar lipids, meanquinone-8(H4) as the only respiratory quinone and iso-C16 : 0, C17:1ω8c, C17:1ω6c, C17 : 0 and C17 : 0 10-methyl (tbsa) as the major fatty acids.
30403584	7	45	theme	respiratory	1079:1089	arg1	acid					896:899	ll-diaminopimelic acid	878:899	ll-diaminopimelic acid in the cell-wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol, four unknown phospholipids and one unknown lipid as the polar lipids, meanquinone-8(H4)	878:1065	Strain S-34T had ll-diaminopimelic acid in the cell-wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol, four unknown phospholipids and one unknown lipid as the polar lipids, meanquinone-8(H4) as the only respiratory quinone and iso-C16 : 0, C17:1ω8c, C17:1ω6c, C17 : 0 and C17 : 0 10-methyl (tbsa) as the major fatty acids.
30403584	7	45	theme	respiratory	1079:1089	arg1	quinone					1091:1097	the only respiratory quinone	1070:1097	the only respiratory quinone	1070:1097	Strain S-34T had ll-diaminopimelic acid in the cell-wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol, four unknown phospholipids and one unknown lipid as the polar lipids, meanquinone-8(H4) as the only respiratory quinone and iso-C16 : 0, C17:1ω8c, C17:1ω6c, C17 : 0 and C17 : 0 10-methyl (tbsa) as the major fatty acids.
30403584	10	46	theme	Nocardioides	1565:1576	arg1	species					1544:1550	a novel species	1536:1550	a novel species	1536:1550	Therefore, strain S-34T represents a novel species of the genus Nocardioides, for which the name Nocardioidessilvaticus sp.
30403584	7	47	theme	unknown	1014:1020	arg1	lipid					1022:1026	one unknown lipid	1010:1026	one unknown lipid	1010:1026	Strain S-34T had ll-diaminopimelic acid in the cell-wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol, four unknown phospholipids and one unknown lipid as the polar lipids, meanquinone-8(H4) as the only respiratory quinone and iso-C16 : 0, C17:1ω8c, C17:1ω6c, C17 : 0 and C17 : 0 10-methyl (tbsa) as the major fatty acids.
30403584	7	47	theme	unknown	1014:1020	arg1	peptidoglycan					918:930	the cell-wall peptidoglycan	904:930	the cell-wall peptidoglycan	904:930	Strain S-34T had ll-diaminopimelic acid in the cell-wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol, four unknown phospholipids and one unknown lipid as the polar lipids, meanquinone-8(H4) as the only respiratory quinone and iso-C16 : 0, C17:1ω8c, C17:1ω6c, C17 : 0 and C17 : 0 10-methyl (tbsa) as the major fatty acids.
30403584	8	48	theme	%	1305:1305	arg1	G+C content					1282:1292	a DNA G+C content	1276:1292	a DNA G+C content of 71.2 mol%	1276:1305	The genome length of strain S-34T was 4.53 Mb containing 52 contigs and with a DNA G+C content of 71.2 mol%.
30403584	9	49	theme	polar	1454:1458	arg1	lipids					1460:1465	polar lipids	1454:1465	polar lipids	1454:1465	Strain S-34T could be distinguished from the other Nocardioides members mainly based on the data of phylogenetic analyses, DNA-DNA hybridization, polar lipids and some biochemical differences.
30403584	10	50	theme	novel	1538:1542	arg1	species					1544:1550	a novel species	1536:1550	a novel species	1536:1550	Therefore, strain S-34T represents a novel species of the genus Nocardioides, for which the name Nocardioidessilvaticus sp.
30403584	6	51	theme	strain	801:806	arg1	S-34T					808:812	strain S-34T	801:812	strain S-34T	801:812	The genome orthoANI value between strain S-34T and N. immobilis CCTCC AB 2017083T was 82.4 %.
30403584	9	52	theme	lipids	1460:1465	arg1	data					1400:1403	the data	1396:1403	the data of phylogenetic analyses, DNA-DNA hybridization, polar lipids and some biochemical differences	1396:1498	Strain S-34T could be distinguished from the other Nocardioides members mainly based on the data of phylogenetic analyses, DNA-DNA hybridization, polar lipids and some biochemical differences.
30403584	4	53	theme	Phylogenetic	468:479	arg1	trees					481:485	Phylogenetic trees	468:485	Phylogenetic trees	468:485	Phylogenetic trees showed that strain S-34T fell within the cluster containing strain S-34T and N. immobilis FLL521T.
30403584	7	54	from	acid	896:899	arg1	diphosphatidylglycerol					933:954	diphosphatidylglycerol	933:954	diphosphatidylglycerol	933:954	Strain S-34T had ll-diaminopimelic acid in the cell-wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol, four unknown phospholipids and one unknown lipid as the polar lipids, meanquinone-8(H4) as the only respiratory quinone and iso-C16 : 0, C17:1ω8c, C17:1ω6c, C17 : 0 and C17 : 0 10-methyl (tbsa) as the major fatty acids.
30403584	7	54	from	acid	896:899	arg1	phospholipids					992:1004	four unknown phospholipids	979:1004	four unknown phospholipids	979:1004	Strain S-34T had ll-diaminopimelic acid in the cell-wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol, four unknown phospholipids and one unknown lipid as the polar lipids, meanquinone-8(H4) as the only respiratory quinone and iso-C16 : 0, C17:1ω8c, C17:1ω6c, C17 : 0 and C17 : 0 10-methyl (tbsa) as the major fatty acids.
30403584	7	54	from	acid	896:899	arg1	phosphatidylglycerol					957:976	phosphatidylglycerol	957:976	phosphatidylglycerol	957:976	Strain S-34T had ll-diaminopimelic acid in the cell-wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol, four unknown phospholipids and one unknown lipid as the polar lipids, meanquinone-8(H4) as the only respiratory quinone and iso-C16 : 0, C17:1ω8c, C17:1ω6c, C17 : 0 and C17 : 0 10-methyl (tbsa) as the major fatty acids.
30403584	7	54	from	acid	896:899	arg1	meanquinone-8					1049:1061	meanquinone-8	1049:1061	meanquinone-8(H4)	1049:1065	Strain S-34T had ll-diaminopimelic acid in the cell-wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol, four unknown phospholipids and one unknown lipid as the polar lipids, meanquinone-8(H4) as the only respiratory quinone and iso-C16 : 0, C17:1ω8c, C17:1ω6c, C17 : 0 and C17 : 0 10-methyl (tbsa) as the major fatty acids.
30403584	7	54	from	acid	896:899	arg1	lipid					1022:1026	one unknown lipid	1010:1026	one unknown lipid	1010:1026	Strain S-34T had ll-diaminopimelic acid in the cell-wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol, four unknown phospholipids and one unknown lipid as the polar lipids, meanquinone-8(H4) as the only respiratory quinone and iso-C16 : 0, C17:1ω8c, C17:1ω6c, C17 : 0 and C17 : 0 10-methyl (tbsa) as the major fatty acids.
30403584	7	54	from	acid	896:899	arg1	peptidoglycan					918:930	the cell-wall peptidoglycan	904:930	the cell-wall peptidoglycan	904:930	Strain S-34T had ll-diaminopimelic acid in the cell-wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol, four unknown phospholipids and one unknown lipid as the polar lipids, meanquinone-8(H4) as the only respiratory quinone and iso-C16 : 0, C17:1ω8c, C17:1ω6c, C17 : 0 and C17 : 0 10-methyl (tbsa) as the major fatty acids.
30403584	5	55	theme	N.	645:646	arg1	2016296T					667:674	N. thalensis CCTCC AB 2016296T	645:674	N. thalensis CCTCC AB 2016296T	645:674	The levels of DNA-DNA relatedness between strain S-34T and N. thalensis CCTCC AB 2016296T and between strain S-34T and N. panacisoli KCTC 19470T were 50.6 and 58.8 %, respectively.
30403584	4	56	theme	strain	499:504	arg1	S-34T					506:510	strain S-34T	499:510	strain S-34T	499:510	Phylogenetic trees showed that strain S-34T fell within the cluster containing strain S-34T and N. immobilis FLL521T.
30403584	10	57	theme	Nocardioidessilvaticus	1598:1619	arg1	sp					1621:1622	the name Nocardioidessilvaticus sp	1589:1622	the name Nocardioidessilvaticus sp	1589:1622	Therefore, strain S-34T represents a novel species of the genus Nocardioides, for which the name Nocardioidessilvaticus sp.
30403584	4	58	contain	containing	536:545	arg2	FLL521T					577:583	N. immobilis FLL521T	564:583	N. immobilis FLL521T	564:583	Phylogenetic trees showed that strain S-34T fell within the cluster containing strain S-34T and N. immobilis FLL521T.
30403584	4	58	contain	containing	536:545	arg1	cluster					528:534	the cluster	524:534	the cluster containing strain S-34T and N. immobilis FLL521T	524:583	Phylogenetic trees showed that strain S-34T fell within the cluster containing strain S-34T and N. immobilis FLL521T.
30403584	4	58	contain	containing	536:545	arg2	S-34T					554:558	strain S-34T	547:558	strain S-34T	547:558	Phylogenetic trees showed that strain S-34T fell within the cluster containing strain S-34T and N. immobilis FLL521T.
30403584	12	59	theme	AB	1688:1689	arg1	2018079T					1691:1698	=KCTC 49137T=CCTCC AB 2018079T	1669:1698	=KCTC 49137T=CCTCC AB 2018079T	1669:1698	The type strain is S-34T (=KCTC 49137T=CCTCC AB 2018079T).
30403584	12	59	theme	AB	1688:1689	arg1	S-34T					1662:1666	S-34T	1662:1666	S-34T (=KCTC 49137T=CCTCC AB 2018079T)	1662:1699	The type strain is S-34T (=KCTC 49137T=CCTCC AB 2018079T).
30403584	9	60	theme	other	1353:1357	arg1	members					1372:1378	the other Nocardioides members	1349:1378	the other Nocardioides members mainly based on the data of phylogenetic analyses, DNA-DNA hybridization, polar lipids and some biochemical differences	1349:1498	Strain S-34T could be distinguished from the other Nocardioides members mainly based on the data of phylogenetic analyses, DNA-DNA hybridization, polar lipids and some biochemical differences.
30403584	2	61	theme	Gram-stain-positive	63:81	arg1	S-34T					124:128	A Gram-stain-positive, non-motile, rod-shaped bacterial strain S-34T	61:128	A Gram-stain-positive, non-motile, rod-shaped bacterial strain S-34T	61:128	A Gram-stain-positive, non-motile, rod-shaped bacterial strain S-34T was isolated from forest soil.
30403584	3	62	theme	highest	271:277	arg1	similarities					279:290	the highest similarities	267:290	the highest similarities to Nocardioides thalensis NCCP-696T (97.3 %) and Nocardioides panacisoliGsoil 346T (97.0 %), Nocardioides litorisoli X-2T (96.5 %) and Nocardioides immobilis FLL521T (96.4 %)	267:465	According to 16S rRNA gene sequence analysis, strain S-34T was related to Nocardioides members and showed the highest similarities to Nocardioides thalensis NCCP-696T (97.3 %) and Nocardioides panacisoliGsoil 346T (97.0 %), Nocardioides litorisoli X-2T (96.5 %) and Nocardioides immobilis FLL521T (96.4 %).
30403584	6	63	theme	orthoANI	778:785	arg1	value					787:791	The genome orthoANI value	767:791	The genome orthoANI value between strain S-34T and N. immobilis CCTCC AB 2017083T	767:847	The genome orthoANI value between strain S-34T and N. immobilis CCTCC AB 2017083T was 82.4 %.
30403584	6	63	theme	orthoANI	778:785	arg1	%					858:858	82.4 %	853:858	82.4 %	853:858	The genome orthoANI value between strain S-34T and N. immobilis CCTCC AB 2017083T was 82.4 %.
30403584	9	64	theme	biochemical	1476:1486	arg1	differences					1488:1498	some biochemical differences	1471:1498	some biochemical differences	1471:1498	Strain S-34T could be distinguished from the other Nocardioides members mainly based on the data of phylogenetic analyses, DNA-DNA hybridization, polar lipids and some biochemical differences.
30403584	12	65	theme	49137T=CCTCC	1675:1686	arg1	2018079T					1691:1698	=KCTC 49137T=CCTCC AB 2018079T	1669:1698	=KCTC 49137T=CCTCC AB 2018079T	1669:1698	The type strain is S-34T (=KCTC 49137T=CCTCC AB 2018079T).
30403584	12	65	theme	49137T=CCTCC	1675:1686	arg1	S-34T					1662:1666	S-34T	1662:1666	S-34T (=KCTC 49137T=CCTCC AB 2018079T)	1662:1699	The type strain is S-34T (=KCTC 49137T=CCTCC AB 2018079T).
30403584	9	66	theme	Nocardioides	1359:1370	arg1	members					1372:1378	the other Nocardioides members	1349:1378	the other Nocardioides members mainly based on the data of phylogenetic analyses, DNA-DNA hybridization, polar lipids and some biochemical differences	1349:1498	Strain S-34T could be distinguished from the other Nocardioides members mainly based on the data of phylogenetic analyses, DNA-DNA hybridization, polar lipids and some biochemical differences.
30403584	4	67	theme	N.	564:565	arg1	FLL521T					577:583	N. immobilis FLL521T	564:583	N. immobilis FLL521T	564:583	Phylogenetic trees showed that strain S-34T fell within the cluster containing strain S-34T and N. immobilis FLL521T.
30403584	5	68	theme	thalensis	648:656	arg1	2016296T					667:674	N. thalensis CCTCC AB 2016296T	645:674	N. thalensis CCTCC AB 2016296T	645:674	The levels of DNA-DNA relatedness between strain S-34T and N. thalensis CCTCC AB 2016296T and between strain S-34T and N. panacisoli KCTC 19470T were 50.6 and 58.8 %, respectively.
30403584	6	69	theme	genome	771:776	arg1	value					787:791	The genome orthoANI value	767:791	The genome orthoANI value between strain S-34T and N. immobilis CCTCC AB 2017083T	767:847	The genome orthoANI value between strain S-34T and N. immobilis CCTCC AB 2017083T was 82.4 %.
30403584	6	69	theme	genome	771:776	arg1	%					858:858	82.4 %	853:858	82.4 %	853:858	The genome orthoANI value between strain S-34T and N. immobilis CCTCC AB 2017083T was 82.4 %.
30403584	8	70	theme	DNA	1278:1280	arg1	G+C content					1282:1292	a DNA G+C content	1276:1292	a DNA G+C content of 71.2 mol%	1276:1305	The genome length of strain S-34T was 4.53 Mb containing 52 contigs and with a DNA G+C content of 71.2 mol%.
30403584	2	71	attach	isolated	134:141	arg1	soil					155:158	forest soil	148:158	forest soil	148:158	A Gram-stain-positive, non-motile, rod-shaped bacterial strain S-34T was isolated from forest soil.
30403584	2	71	attach	isolated	134:141	arg2	S-34T					124:128	A Gram-stain-positive, non-motile, rod-shaped bacterial strain S-34T	61:128	A Gram-stain-positive, non-motile, rod-shaped bacterial strain S-34T	61:128	A Gram-stain-positive, non-motile, rod-shaped bacterial strain S-34T was isolated from forest soil.
30403584	10	72	theme	genus	1559:1563	arg1	Nocardioides					1565:1576	the genus Nocardioides	1555:1576	the genus Nocardioides	1555:1576	Therefore, strain S-34T represents a novel species of the genus Nocardioides, for which the name Nocardioidessilvaticus sp.
30403584	5	73	theme	N.	705:706	arg1	19470T					724:729	N. panacisoli KCTC 19470T	705:729	N. panacisoli KCTC 19470T	705:729	The levels of DNA-DNA relatedness between strain S-34T and N. thalensis CCTCC AB 2016296T and between strain S-34T and N. panacisoli KCTC 19470T were 50.6 and 58.8 %, respectively.
30403584	8	74	theme	71.2 mol	1297:1304	arg1	%					1305:1305	71.2 mol%	1297:1305	71.2 mol%	1297:1305	The genome length of strain S-34T was 4.53 Mb containing 52 contigs and with a DNA G+C content of 71.2 mol%.
30403584	12	75	theme	type	1647:1650	arg1	S-34T					1662:1666	S-34T	1662:1666	S-34T (=KCTC 49137T=CCTCC AB 2018079T)	1662:1699	The type strain is S-34T (=KCTC 49137T=CCTCC AB 2018079T).
30403584	12	75	theme	type	1647:1650	arg1	strain					1652:1657	The type strain	1643:1657	The type strain	1643:1657	The type strain is S-34T (=KCTC 49137T=CCTCC AB 2018079T).
30403584	10	76	theme	name	1593:1596	arg1	sp					1621:1622	the name Nocardioidessilvaticus sp	1589:1622	the name Nocardioidessilvaticus sp	1589:1622	Therefore, strain S-34T represents a novel species of the genus Nocardioides, for which the name Nocardioidessilvaticus sp.
30403584	5	77	theme	panacisoli	708:717	arg1	19470T					724:729	N. panacisoli KCTC 19470T	705:729	N. panacisoli KCTC 19470T	705:729	The levels of DNA-DNA relatedness between strain S-34T and N. thalensis CCTCC AB 2016296T and between strain S-34T and N. panacisoli KCTC 19470T were 50.6 and 58.8 %, respectively.
30403584	7	78	theme	cell-wall	908:916	arg1	diphosphatidylglycerol					933:954	diphosphatidylglycerol	933:954	diphosphatidylglycerol	933:954	Strain S-34T had ll-diaminopimelic acid in the cell-wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol, four unknown phospholipids and one unknown lipid as the polar lipids, meanquinone-8(H4) as the only respiratory quinone and iso-C16 : 0, C17:1ω8c, C17:1ω6c, C17 : 0 and C17 : 0 10-methyl (tbsa) as the major fatty acids.
30403584	7	78	theme	cell-wall	908:916	arg1	phospholipids					992:1004	four unknown phospholipids	979:1004	four unknown phospholipids	979:1004	Strain S-34T had ll-diaminopimelic acid in the cell-wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol, four unknown phospholipids and one unknown lipid as the polar lipids, meanquinone-8(H4) as the only respiratory quinone and iso-C16 : 0, C17:1ω8c, C17:1ω6c, C17 : 0 and C17 : 0 10-methyl (tbsa) as the major fatty acids.
30403584	7	78	theme	cell-wall	908:916	arg1	phosphatidylglycerol					957:976	phosphatidylglycerol	957:976	phosphatidylglycerol	957:976	Strain S-34T had ll-diaminopimelic acid in the cell-wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol, four unknown phospholipids and one unknown lipid as the polar lipids, meanquinone-8(H4) as the only respiratory quinone and iso-C16 : 0, C17:1ω8c, C17:1ω6c, C17 : 0 and C17 : 0 10-methyl (tbsa) as the major fatty acids.
30403584	7	78	theme	cell-wall	908:916	arg1	meanquinone-8					1049:1061	meanquinone-8	1049:1061	meanquinone-8(H4)	1049:1065	Strain S-34T had ll-diaminopimelic acid in the cell-wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol, four unknown phospholipids and one unknown lipid as the polar lipids, meanquinone-8(H4) as the only respiratory quinone and iso-C16 : 0, C17:1ω8c, C17:1ω6c, C17 : 0 and C17 : 0 10-methyl (tbsa) as the major fatty acids.
30403584	7	78	theme	cell-wall	908:916	arg1	lipid					1022:1026	one unknown lipid	1010:1026	one unknown lipid	1010:1026	Strain S-34T had ll-diaminopimelic acid in the cell-wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol, four unknown phospholipids and one unknown lipid as the polar lipids, meanquinone-8(H4) as the only respiratory quinone and iso-C16 : 0, C17:1ω8c, C17:1ω6c, C17 : 0 and C17 : 0 10-methyl (tbsa) as the major fatty acids.
30403584	7	78	theme	cell-wall	908:916	arg1	peptidoglycan					918:930	the cell-wall peptidoglycan	904:930	the cell-wall peptidoglycan	904:930	Strain S-34T had ll-diaminopimelic acid in the cell-wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol, four unknown phospholipids and one unknown lipid as the polar lipids, meanquinone-8(H4) as the only respiratory quinone and iso-C16 : 0, C17:1ω8c, C17:1ω6c, C17 : 0 and C17 : 0 10-methyl (tbsa) as the major fatty acids.
30403584	7	79	theme	 0	1153:1154	arg1	tbsa					1167:1170	tbsa	1167:1170	tbsa	1167:1170	Strain S-34T had ll-diaminopimelic acid in the cell-wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol, four unknown phospholipids and one unknown lipid as the polar lipids, meanquinone-8(H4) as the only respiratory quinone and iso-C16 : 0, C17:1ω8c, C17:1ω6c, C17 : 0 and C17 : 0 10-methyl (tbsa) as the major fatty acids.
30403584	7	79	theme	 0	1153:1154	arg1	10-methyl					1156:1164	 0 10-methyl	1153:1164	 0 10-methyl (tbsa) as the major fatty acids	1153:1196	Strain S-34T had ll-diaminopimelic acid in the cell-wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol, four unknown phospholipids and one unknown lipid as the polar lipids, meanquinone-8(H4) as the only respiratory quinone and iso-C16 : 0, C17:1ω8c, C17:1ω6c, C17 : 0 and C17 : 0 10-methyl (tbsa) as the major fatty acids.
30403584	4	80	theme	immobilis	567:575	arg1	FLL521T					577:583	N. immobilis FLL521T	564:583	N. immobilis FLL521T	564:583	Phylogenetic trees showed that strain S-34T fell within the cluster containing strain S-34T and N. immobilis FLL521T.
30444473	12	0	theme	CPCC	1221:1224	arg1	204380T					1226:1232	strain CPCC 204380T	1214:1232	strain CPCC 204380T	1214:1232	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CPCC 204380T should be placed in the family Micromonosporaceae, in which it formed a distinct lineage next to the genera Rhizocola, Catellatospora, Catelliglobosispora, Hamadaea and Allocatelliglobosispora.
30444473	13	1	theme	other	1770:1774	arg1	species					1794:1800	other validly described species	1770:1800	other validly described species	1770:1800	It shared the highest 16S rRNA gene sequence similarities with Rhizocola hellebori K12-0602T (96.1 %), Catellatospora chokoriensis 2-25/1T (95.9 %), Catelliglobosispora koreensis DSM 44566T (95.9 %), Hamadaea tsunoensis DSM 44101T (95.3 %) and Allocatelliglobosispora scoriae Sco-B14 T (94.2 %), and less than 94.0 % sequence similarity with other validly described species.
30444473	1	2	theme	rhizosphere	98:108	arg1	soil					110:113	rhizosphere soil	98:113	rhizosphere soil of the plant Calligonum mongolicum	98:148	nov., a new member of Micromonosporaceae isolated from rhizosphere soil of the plant Calligonum mongolicum.
30444473	16	3	theme	type	2149:2152	arg1	species					2154:2160	the type species	2145:2160	the type species	2145:2160	CPCC 204380T (=DSM 102292T=KCTC 39746 T) is the type strain of the type species.
30444473	5	4	theme	diagnostic	663:672	arg1	acid					618:621	meso-diaminopimelic acid	598:621	meso-diaminopimelic acid	598:621	The peptidoglycan contained meso-diaminopimelic acid and 3-hydroxydiaminopimelic acid as the diagnostic amino acids.
30444473	5	4	theme	diagnostic	663:672	arg1	acids					680:684	the diagnostic amino acids	659:684	the diagnostic amino acids	659:684	The peptidoglycan contained meso-diaminopimelic acid and 3-hydroxydiaminopimelic acid as the diagnostic amino acids.
30444473	5	4	theme	diagnostic	663:672	arg1	acid					651:654	3-hydroxydiaminopimelic acid	627:654	3-hydroxydiaminopimelic acid	627:654	The peptidoglycan contained meso-diaminopimelic acid and 3-hydroxydiaminopimelic acid as the diagnostic amino acids.
30444473	2	5	theme	rhizosphere	248:258	arg1	sample					265:270	a rhizosphere soil sample	246:270	a rhizosphere soil sample of the plant Calligonum mongolicum collected from Xinjiang Province, China	246:345	The taxonomic position of an actinobacterium, designated CPCC 204380T, which was isolated from a rhizosphere soil sample of the plant Calligonum mongolicum collected from Xinjiang Province, China, was established using a polyphasic approach.
30444473	13	6	dep	Allocatelliglobosispora	1672:1694	arg1	T					1712:1712	Sco-B14 T	1704:1712	Allocatelliglobosispora scoriae Sco-B14 T (94.2 %)	1672:1721	It shared the highest 16S rRNA gene sequence similarities with Rhizocola hellebori K12-0602T (96.1 %), Catellatospora chokoriensis 2-25/1T (95.9 %), Catelliglobosispora koreensis DSM 44566T (95.9 %), Hamadaea tsunoensis DSM 44101T (95.3 %) and Allocatelliglobosispora scoriae Sco-B14 T (94.2 %), and less than 94.0 % sequence similarity with other validly described species.
30444473	13	6	dep	Allocatelliglobosispora	1672:1694	arg1	%					1720:1720	94.2 %	1715:1720	94.2 %	1715:1720	It shared the highest 16S rRNA gene sequence similarities with Rhizocola hellebori K12-0602T (96.1 %), Catellatospora chokoriensis 2-25/1T (95.9 %), Catelliglobosispora koreensis DSM 44566T (95.9 %), Hamadaea tsunoensis DSM 44101T (95.3 %) and Allocatelliglobosispora scoriae Sco-B14 T (94.2 %), and less than 94.0 % sequence similarity with other validly described species.
30444473	13	6	dep	Allocatelliglobosispora	1672:1694	arg1	scoriae					1696:1702	Allocatelliglobosispora scoriae Sco-B14 T (94.2 %)	1672:1721	Allocatelliglobosispora scoriae Sco-B14 T (94.2 %)	1672:1721	It shared the highest 16S rRNA gene sequence similarities with Rhizocola hellebori K12-0602T (96.1 %), Catellatospora chokoriensis 2-25/1T (95.9 %), Catelliglobosispora koreensis DSM 44566T (95.9 %), Hamadaea tsunoensis DSM 44101T (95.3 %) and Allocatelliglobosispora scoriae Sco-B14 T (94.2 %), and less than 94.0 % sequence similarity with other validly described species.
30444473	16	7	theme	type	2130:2133	arg1	strain					2135:2140	the type strain	2126:2140	the type strain of the type species	2126:2160	CPCC 204380T (=DSM 102292T=KCTC 39746 T) is the type strain of the type species.
30444473	16	7	theme	type	2130:2133	arg1	204380T					2087:2093	CPCC 204380T	2082:2093	CPCC 204380T (=DSM 102292T=KCTC 39746 T)	2082:2121	CPCC 204380T (=DSM 102292T=KCTC 39746 T) is the type strain of the type species.
30444473	16	8	theme	CPCC	2082:2085	arg1	strain					2135:2140	the type strain	2126:2140	the type strain of the type species	2126:2160	CPCC 204380T (=DSM 102292T=KCTC 39746 T) is the type strain of the type species.
30444473	16	8	theme	CPCC	2082:2085	arg1	T					2120:2120	=DSM 102292T=KCTC 39746 T	2096:2120	=DSM 102292T=KCTC 39746 T	2096:2120	CPCC 204380T (=DSM 102292T=KCTC 39746 T) is the type strain of the type species.
30444473	16	8	theme	CPCC	2082:2085	arg1	204380T					2087:2093	CPCC 204380T	2082:2093	CPCC 204380T (=DSM 102292T=KCTC 39746 T)	2082:2121	CPCC 204380T (=DSM 102292T=KCTC 39746 T) is the type strain of the type species.
30444473	2	9	theme	Calligonum	285:294	arg1	mongolicum					296:305	the plant Calligonum mongolicum	275:305	the plant Calligonum mongolicum collected from Xinjiang Province, China	275:345	The taxonomic position of an actinobacterium, designated CPCC 204380T, which was isolated from a rhizosphere soil sample of the plant Calligonum mongolicum collected from Xinjiang Province, China, was established using a polyphasic approach.
30444473	14	10	from	genus	1962:1966	arg1	Micromonosporaceae					1982:1999	the family Micromonosporaceae	1971:1999	the family Micromonosporaceae	1971:1999	The combination of phylogenetic analysis and phenotypic characteristics supported the proposal of strain CPCC 204380T as representing a novel species of a new genus in the family Micromonosporaceae, for which the name Allorhizocola rhizosphaerae gen. nov., sp.
30444473	14	11	theme	new	1958:1960	arg1	genus					1962:1966	a new genus	1956:1966	a new genus in the family Micromonosporaceae	1956:1999	The combination of phylogenetic analysis and phenotypic characteristics supported the proposal of strain CPCC 204380T as representing a novel species of a new genus in the family Micromonosporaceae, for which the name Allorhizocola rhizosphaerae gen. nov., sp.
30444473	14	12	theme	phenotypic	1848:1857	arg1	characteristics					1859:1873	phenotypic characteristics	1848:1873	phenotypic characteristics	1848:1873	The combination of phylogenetic analysis and phenotypic characteristics supported the proposal of strain CPCC 204380T as representing a novel species of a new genus in the family Micromonosporaceae, for which the name Allorhizocola rhizosphaerae gen. nov., sp.
30444473	5	13	contain	contained	588:596	arg2	acids					680:684	the diagnostic amino acids	659:684	the diagnostic amino acids	659:684	The peptidoglycan contained meso-diaminopimelic acid and 3-hydroxydiaminopimelic acid as the diagnostic amino acids.
30444473	5	13	contain	contained	588:596	arg1	peptidoglycan					574:586	The peptidoglycan	570:586	The peptidoglycan	570:586	The peptidoglycan contained meso-diaminopimelic acid and 3-hydroxydiaminopimelic acid as the diagnostic amino acids.
30444473	5	13	contain	contained	588:596	arg2	acid					651:654	3-hydroxydiaminopimelic acid	627:654	3-hydroxydiaminopimelic acid	627:654	The peptidoglycan contained meso-diaminopimelic acid and 3-hydroxydiaminopimelic acid as the diagnostic amino acids.
30444473	5	13	contain	contained	588:596	arg2	acid					618:621	meso-diaminopimelic acid	598:621	meso-diaminopimelic acid	598:621	The peptidoglycan contained meso-diaminopimelic acid and 3-hydroxydiaminopimelic acid as the diagnostic amino acids.
30444473	16	14	theme	102292T=KCTC	2101:2112	arg1	T					2120:2120	=DSM 102292T=KCTC 39746 T	2096:2120	=DSM 102292T=KCTC 39746 T	2096:2120	CPCC 204380T (=DSM 102292T=KCTC 39746 T) is the type strain of the type species.
30444473	16	14	theme	102292T=KCTC	2101:2112	arg1	204380T					2087:2093	CPCC 204380T	2082:2093	CPCC 204380T (=DSM 102292T=KCTC 39746 T)	2082:2121	CPCC 204380T (=DSM 102292T=KCTC 39746 T) is the type strain of the type species.
30444473	10	15	theme	fatty	1048:1052	arg1	acids					1054:1058	The major fatty acids	1038:1058	The major fatty acids	1038:1058	The major fatty acids were iso-C15 : 0, iso-C16 : 0 and C17 : 1ω9c.
30444473	10	15	theme	fatty	1048:1052	arg1	 0					1074:1075	 0	1074:1075	 0	1074:1075	The major fatty acids were iso-C15 : 0, iso-C16 : 0 and C17 : 1ω9c.
30444473	10	15	theme	fatty	1048:1052	arg1	iso-C15 					1065:1072	iso-C15 	1065:1072	iso-C15 	1065:1072	The major fatty acids were iso-C15 : 0, iso-C16 : 0 and C17 : 1ω9c.
30444473	14	16	theme	analysis	1835:1842	arg1	combination					1807:1817	The combination	1803:1817	The combination of phylogenetic analysis and phenotypic characteristics	1803:1873	The combination of phylogenetic analysis and phenotypic characteristics supported the proposal of strain CPCC 204380T as representing a novel species of a new genus in the family Micromonosporaceae, for which the name Allorhizocola rhizosphaerae gen. nov., sp.
30444473	2	17	theme	CPCC	208:211	arg1	204380T					213:219	CPCC 204380T	208:219	CPCC 204380T	208:219	The taxonomic position of an actinobacterium, designated CPCC 204380T, which was isolated from a rhizosphere soil sample of the plant Calligonum mongolicum collected from Xinjiang Province, China, was established using a polyphasic approach.
30444473	14	18	from	species	1945:1951	arg1	Micromonosporaceae					1982:1999	the family Micromonosporaceae	1971:1999	the family Micromonosporaceae	1971:1999	The combination of phylogenetic analysis and phenotypic characteristics supported the proposal of strain CPCC 204380T as representing a novel species of a new genus in the family Micromonosporaceae, for which the name Allorhizocola rhizosphaerae gen. nov., sp.
30444473	6	19	theme	acyl	691:694	arg1	glycolyl					726:733	glycolyl	726:733	glycolyl	726:733	The acyl type of the peptidoglycan was glycolyl.
30444473	6	19	theme	acyl	691:694	arg1	type					696:699	The acyl type	687:699	The acyl type of the peptidoglycan	687:720	The acyl type of the peptidoglycan was glycolyl.
30444473	6	20	theme	peptidoglycan	708:720	arg1	glycolyl					726:733	glycolyl	726:733	glycolyl	726:733	The acyl type of the peptidoglycan was glycolyl.
30444473	6	20	theme	peptidoglycan	708:720	arg1	type					696:699	The acyl type	687:699	The acyl type of the peptidoglycan	687:720	The acyl type of the peptidoglycan was glycolyl.
30444473	10	21	dep	iso-C15 	1065:1072	arg1	acids					1054:1058	The major fatty acids	1038:1058	The major fatty acids	1038:1058	The major fatty acids were iso-C15 : 0, iso-C16 : 0 and C17 : 1ω9c.
30444473	10	21	dep	iso-C15 	1065:1072	arg1	iso-C16 					1078:1085	iso-C16 	1078:1085	iso-C16 	1078:1085	The major fatty acids were iso-C15 : 0, iso-C16 : 0 and C17 : 1ω9c.
30444473	10	21	dep	iso-C15 	1065:1072	arg1	 0					1074:1075	 0	1074:1075	 0	1074:1075	The major fatty acids were iso-C15 : 0, iso-C16 : 0 and C17 : 1ω9c.
30444473	10	21	dep	iso-C15 	1065:1072	arg1	iso-C15 					1065:1072	iso-C15 	1065:1072	iso-C15 	1065:1072	The major fatty acids were iso-C15 : 0, iso-C16 : 0 and C17 : 1ω9c.
30444473	13	22	theme	gene	1459:1462	arg1	similarities					1473:1484	the highest 16S rRNA gene sequence similarities	1438:1484	the highest 16S rRNA gene sequence similarities	1438:1484	It shared the highest 16S rRNA gene sequence similarities with Rhizocola hellebori K12-0602T (96.1 %), Catellatospora chokoriensis 2-25/1T (95.9 %), Catelliglobosispora koreensis DSM 44566T (95.9 %), Hamadaea tsunoensis DSM 44101T (95.3 %) and Allocatelliglobosispora scoriae Sco-B14 T (94.2 %), and less than 94.0 % sequence similarity with other validly described species.
30444473	12	23	theme	16S	1176:1178	arg1	sequences					1190:1198	16S rRNA gene sequences	1176:1198	16S rRNA gene sequences	1176:1198	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CPCC 204380T should be placed in the family Micromonosporaceae, in which it formed a distinct lineage next to the genera Rhizocola, Catellatospora, Catelliglobosispora, Hamadaea and Allocatelliglobosispora.
30444473	1	24	theme	Calligonum	128:137	arg1	mongolicum					139:148	the plant Calligonum mongolicum	118:148	the plant Calligonum mongolicum	118:148	nov., a new member of Micromonosporaceae isolated from rhizosphere soil of the plant Calligonum mongolicum.
30444473	10	25	dep	 0	1074:1075	arg1	 0					1087:1088	 0	1087:1088	 0	1087:1088	The major fatty acids were iso-C15 : 0, iso-C16 : 0 and C17 : 1ω9c.
30444473	10	25	dep	 0	1074:1075	arg1	 1ω9c					1099:1103	 1ω9c	1099:1103	 1ω9c	1099:1103	The major fatty acids were iso-C15 : 0, iso-C16 : 0 and C17 : 1ω9c.
30444473	10	25	dep	 0	1074:1075	arg1	C17 					1094:1097	C17 	1094:1097	C17 	1094:1097	The major fatty acids were iso-C15 : 0, iso-C16 : 0 and C17 : 1ω9c.
30444473	12	26	theme	gene	1185:1188	arg1	sequences					1190:1198	16S rRNA gene sequences	1176:1198	16S rRNA gene sequences	1176:1198	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CPCC 204380T should be placed in the family Micromonosporaceae, in which it formed a distinct lineage next to the genera Rhizocola, Catellatospora, Catelliglobosispora, Hamadaea and Allocatelliglobosispora.
30444473	12	27	theme	Phylogenetic	1145:1156	arg1	analysis					1158:1165	Phylogenetic analysis	1145:1165	Phylogenetic analysis based on 16S rRNA gene sequences	1145:1198	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CPCC 204380T should be placed in the family Micromonosporaceae, in which it formed a distinct lineage next to the genera Rhizocola, Catellatospora, Catelliglobosispora, Hamadaea and Allocatelliglobosispora.
30444473	14	28	from	Micromonosporaceae	1982:1999	arg1	species					1945:1951	a novel species	1937:1951	a novel species	1937:1951	The combination of phylogenetic analysis and phenotypic characteristics supported the proposal of strain CPCC 204380T as representing a novel species of a new genus in the family Micromonosporaceae, for which the name Allorhizocola rhizosphaerae gen. nov., sp.
30444473	14	29	theme	family	1975:1980	arg1	Micromonosporaceae					1982:1999	the family Micromonosporaceae	1971:1999	the family Micromonosporaceae	1971:1999	The combination of phylogenetic analysis and phenotypic characteristics supported the proposal of strain CPCC 204380T as representing a novel species of a new genus in the family Micromonosporaceae, for which the name Allorhizocola rhizosphaerae gen. nov., sp.
30444473	3	30	theme	aged	457:460	arg1	hyphae					462:467	aged hyphae	457:467	aged hyphae	457:467	Vegetative hyphae developed well and globose bodies formed from aged hyphae.
30444473	2	31	theme	polyphasic	372:381	arg1	approach					383:390	a polyphasic approach	370:390	a polyphasic approach	370:390	The taxonomic position of an actinobacterium, designated CPCC 204380T, which was isolated from a rhizosphere soil sample of the plant Calligonum mongolicum collected from Xinjiang Province, China, was established using a polyphasic approach.
30444473	4	32	theme	Spore	470:474	arg1	chains					476:481	Spore chains	470:481	Spore chains that differentiated from the vegetative hyphae	470:528	Spore chains that differentiated from the vegetative hyphae contained non-motile rod-shaped spores.
30444473	0	33	theme	Allorhizocola	0:12	arg1	nov.					33:36	Allorhizocola rhizosphaerae gen. nov.	0:36	Allorhizocola rhizosphaerae gen. nov.	0:36	Allorhizocola rhizosphaerae gen. nov., sp.
30444473	5	34	theme	meso-diaminopimelic	598:616	arg1	acid					618:621	meso-diaminopimelic acid	598:621	meso-diaminopimelic acid	598:621	The peptidoglycan contained meso-diaminopimelic acid and 3-hydroxydiaminopimelic acid as the diagnostic amino acids.
30444473	5	34	theme	meso-diaminopimelic	598:616	arg1	acid					651:654	3-hydroxydiaminopimelic acid	627:654	3-hydroxydiaminopimelic acid	627:654	The peptidoglycan contained meso-diaminopimelic acid and 3-hydroxydiaminopimelic acid as the diagnostic amino acids.
30444473	5	34	theme	meso-diaminopimelic	598:616	arg1	acids					680:684	the diagnostic amino acids	659:684	the diagnostic amino acids	659:684	The peptidoglycan contained meso-diaminopimelic acid and 3-hydroxydiaminopimelic acid as the diagnostic amino acids.
30444473	1	35	theme	Micromonosporaceae	65:82	arg1	member					55:60	a new member	49:60	a new member of Micromonosporaceae isolated from rhizosphere soil of the plant Calligonum mongolicum	49:148	nov., a new member of Micromonosporaceae isolated from rhizosphere soil of the plant Calligonum mongolicum.
30444473	1	35	theme	Micromonosporaceae	65:82	arg1	nov.					43:46	nov.	43:46	nov.	43:46	nov., a new member of Micromonosporaceae isolated from rhizosphere soil of the plant Calligonum mongolicum.
30444473	4	36	theme	rod-shaped	551:560	arg1	spores					562:567	non-motile rod-shaped spores	540:567	non-motile rod-shaped spores	540:567	Spore chains that differentiated from the vegetative hyphae contained non-motile rod-shaped spores.
30444473	14	37	theme	gen.	2049:2052	arg1	nov.					2054:2057	Allorhizocola rhizosphaerae gen. nov.	2021:2057	the name Allorhizocola rhizosphaerae gen. nov.	2012:2057	The combination of phylogenetic analysis and phenotypic characteristics supported the proposal of strain CPCC 204380T as representing a novel species of a new genus in the family Micromonosporaceae, for which the name Allorhizocola rhizosphaerae gen. nov., sp.
30444473	13	38	theme	described	1784:1792	arg1	species					1794:1800	other validly described species	1770:1800	other validly described species	1770:1800	It shared the highest 16S rRNA gene sequence similarities with Rhizocola hellebori K12-0602T (96.1 %), Catellatospora chokoriensis 2-25/1T (95.9 %), Catelliglobosispora koreensis DSM 44566T (95.9 %), Hamadaea tsunoensis DSM 44101T (95.3 %) and Allocatelliglobosispora scoriae Sco-B14 T (94.2 %), and less than 94.0 % sequence similarity with other validly described species.
30444473	12	39	dep	genera	1335:1340	arg1	Catellatospora					1353:1366	Catellatospora	1353:1366	Catellatospora	1353:1366	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CPCC 204380T should be placed in the family Micromonosporaceae, in which it formed a distinct lineage next to the genera Rhizocola, Catellatospora, Catelliglobosispora, Hamadaea and Allocatelliglobosispora.
30444473	12	39	dep	genera	1335:1340	arg1	Allocatelliglobosispora					1403:1425	Allocatelliglobosispora	1403:1425	Allocatelliglobosispora	1403:1425	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CPCC 204380T should be placed in the family Micromonosporaceae, in which it formed a distinct lineage next to the genera Rhizocola, Catellatospora, Catelliglobosispora, Hamadaea and Allocatelliglobosispora.
30444473	12	39	dep	genera	1335:1340	arg1	Hamadaea					1390:1397	Hamadaea	1390:1397	Hamadaea	1390:1397	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CPCC 204380T should be placed in the family Micromonosporaceae, in which it formed a distinct lineage next to the genera Rhizocola, Catellatospora, Catelliglobosispora, Hamadaea and Allocatelliglobosispora.
30444473	0	40	theme	gen.	28:31	arg1	nov.					33:36	Allorhizocola rhizosphaerae gen. nov.	0:36	Allorhizocola rhizosphaerae gen. nov.	0:36	Allorhizocola rhizosphaerae gen. nov., sp.
30444473	2	41	theme	soil	260:263	arg1	sample					265:270	a rhizosphere soil sample	246:270	a rhizosphere soil sample of the plant Calligonum mongolicum collected from Xinjiang Province, China	246:345	The taxonomic position of an actinobacterium, designated CPCC 204380T, which was isolated from a rhizosphere soil sample of the plant Calligonum mongolicum collected from Xinjiang Province, China, was established using a polyphasic approach.
30444473	3	42	theme	Vegetative	393:402	arg1	hyphae					404:409	Vegetative hyphae	393:409	Vegetative hyphae	393:409	Vegetative hyphae developed well and globose bodies formed from aged hyphae.
30444473	14	43	theme	Allorhizocola	2021:2033	arg1	nov.					2054:2057	Allorhizocola rhizosphaerae gen. nov.	2021:2057	the name Allorhizocola rhizosphaerae gen. nov.	2012:2057	The combination of phylogenetic analysis and phenotypic characteristics supported the proposal of strain CPCC 204380T as representing a novel species of a new genus in the family Micromonosporaceae, for which the name Allorhizocola rhizosphaerae gen. nov., sp.
30444473	13	44	dep	similarity	1754:1763	arg1	%					1743:1743	%	1743:1743	%	1743:1743	It shared the highest 16S rRNA gene sequence similarities with Rhizocola hellebori K12-0602T (96.1 %), Catellatospora chokoriensis 2-25/1T (95.9 %), Catelliglobosispora koreensis DSM 44566T (95.9 %), Hamadaea tsunoensis DSM 44101T (95.3 %) and Allocatelliglobosispora scoriae Sco-B14 T (94.2 %), and less than 94.0 % sequence similarity with other validly described species.
30444473	14	45	theme	novel	1939:1943	arg1	species					1945:1951	a novel species	1937:1951	a novel species	1937:1951	The combination of phylogenetic analysis and phenotypic characteristics supported the proposal of strain CPCC 204380T as representing a novel species of a new genus in the family Micromonosporaceae, for which the name Allorhizocola rhizosphaerae gen. nov., sp.
30444473	12	46	theme	family	1258:1263	arg1	Micromonosporaceae					1265:1282	the family Micromonosporaceae	1254:1282	the family Micromonosporaceae	1254:1282	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CPCC 204380T should be placed in the family Micromonosporaceae, in which it formed a distinct lineage next to the genera Rhizocola, Catellatospora, Catelliglobosispora, Hamadaea and Allocatelliglobosispora.
30444473	4	47	theme	vegetative	512:521	arg1	hyphae					523:528	the vegetative hyphae	508:528	the vegetative hyphae	508:528	Spore chains that differentiated from the vegetative hyphae contained non-motile rod-shaped spores.
30444473	7	48	theme	whole-cell	789:798	arg1	hydrolysates					800:811	whole-cell hydrolysates	789:811	whole-cell hydrolysates	789:811	Glucose, mannose, ribose and xylose were detected in whole-cell hydrolysates.
30444473	13	49	dep	%	1743:1743	arg1	94.0 					1738:1742	94.0 	1738:1742	94.0 	1738:1742	It shared the highest 16S rRNA gene sequence similarities with Rhizocola hellebori K12-0602T (96.1 %), Catellatospora chokoriensis 2-25/1T (95.9 %), Catelliglobosispora koreensis DSM 44566T (95.9 %), Hamadaea tsunoensis DSM 44101T (95.3 %) and Allocatelliglobosispora scoriae Sco-B14 T (94.2 %), and less than 94.0 % sequence similarity with other validly described species.
30444473	11	50	theme	genomic	1110:1116	arg1	content					1122:1128	The genomic G+C content	1106:1128	The genomic G+C content	1106:1128	The genomic G+C content was 64.9 mol%.
30444473	11	50	theme	genomic	1110:1116	arg1	%					1142:1142	64.9 mol%	1134:1142	64.9 mol%	1134:1142	The genomic G+C content was 64.9 mol%.
30444473	13	51	theme	16S	1450:1452	arg1	rRNA					1454:1457	the highest 16S rRNA	1438:1457	the highest 16S rRNA gene sequence similarities	1438:1484	It shared the highest 16S rRNA gene sequence similarities with Rhizocola hellebori K12-0602T (96.1 %), Catellatospora chokoriensis 2-25/1T (95.9 %), Catelliglobosispora koreensis DSM 44566T (95.9 %), Hamadaea tsunoensis DSM 44101T (95.3 %) and Allocatelliglobosispora scoriae Sco-B14 T (94.2 %), and less than 94.0 % sequence similarity with other validly described species.
30444473	14	52	theme	204380T	1913:1919	arg1	proposal					1889:1896	the proposal	1885:1896	the proposal of strain CPCC 204380T	1885:1919	The combination of phylogenetic analysis and phenotypic characteristics supported the proposal of strain CPCC 204380T as representing a novel species of a new genus in the family Micromonosporaceae, for which the name Allorhizocola rhizosphaerae gen. nov., sp.
30444473	13	53	theme	sequence	1745:1752	arg1	similarity					1754:1763	less than 94.0 % sequence similarity	1728:1763	less than 94.0 % sequence similarity	1728:1763	It shared the highest 16S rRNA gene sequence similarities with Rhizocola hellebori K12-0602T (96.1 %), Catellatospora chokoriensis 2-25/1T (95.9 %), Catelliglobosispora koreensis DSM 44566T (95.9 %), Hamadaea tsunoensis DSM 44101T (95.3 %) and Allocatelliglobosispora scoriae Sco-B14 T (94.2 %), and less than 94.0 % sequence similarity with other validly described species.
30444473	14	54	theme	strain	1901:1906	arg1	204380T					1913:1919	strain CPCC 204380T	1901:1919	strain CPCC 204380T	1901:1919	The combination of phylogenetic analysis and phenotypic characteristics supported the proposal of strain CPCC 204380T as representing a novel species of a new genus in the family Micromonosporaceae, for which the name Allorhizocola rhizosphaerae gen. nov., sp.
30444473	9	55	theme	lipid	904:908	arg1	profile					910:916	The polar lipid profile	894:916	The polar lipid profile	894:916	The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside.
30444473	13	56	dep	Hamadaea	1628:1635	arg1	44101T					1652:1657	DSM 44101T	1648:1657	Hamadaea tsunoensis DSM 44101T (95.3 %)	1628:1666	It shared the highest 16S rRNA gene sequence similarities with Rhizocola hellebori K12-0602T (96.1 %), Catellatospora chokoriensis 2-25/1T (95.9 %), Catelliglobosispora koreensis DSM 44566T (95.9 %), Hamadaea tsunoensis DSM 44101T (95.3 %) and Allocatelliglobosispora scoriae Sco-B14 T (94.2 %), and less than 94.0 % sequence similarity with other validly described species.
30444473	13	56	dep	Hamadaea	1628:1635	arg1	%					1665:1665	95.3 %	1660:1665	95.3 %	1660:1665	It shared the highest 16S rRNA gene sequence similarities with Rhizocola hellebori K12-0602T (96.1 %), Catellatospora chokoriensis 2-25/1T (95.9 %), Catelliglobosispora koreensis DSM 44566T (95.9 %), Hamadaea tsunoensis DSM 44101T (95.3 %) and Allocatelliglobosispora scoriae Sco-B14 T (94.2 %), and less than 94.0 % sequence similarity with other validly described species.
30444473	13	56	dep	Hamadaea	1628:1635	arg1	tsunoensis					1637:1646	Hamadaea tsunoensis DSM 44101T (95.3 %)	1628:1666	Hamadaea tsunoensis DSM 44101T (95.3 %)	1628:1666	It shared the highest 16S rRNA gene sequence similarities with Rhizocola hellebori K12-0602T (96.1 %), Catellatospora chokoriensis 2-25/1T (95.9 %), Catelliglobosispora koreensis DSM 44566T (95.9 %), Hamadaea tsunoensis DSM 44101T (95.3 %) and Allocatelliglobosispora scoriae Sco-B14 T (94.2 %), and less than 94.0 % sequence similarity with other validly described species.
30444473	12	57	theme	strain	1214:1219	arg1	204380T					1226:1232	strain CPCC 204380T	1214:1232	strain CPCC 204380T	1214:1232	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CPCC 204380T should be placed in the family Micromonosporaceae, in which it formed a distinct lineage next to the genera Rhizocola, Catellatospora, Catelliglobosispora, Hamadaea and Allocatelliglobosispora.
30444473	4	58	contain	contained	530:538	arg2	spores					562:567	non-motile rod-shaped spores	540:567	non-motile rod-shaped spores	540:567	Spore chains that differentiated from the vegetative hyphae contained non-motile rod-shaped spores.
30444473	4	58	contain	contained	530:538	arg1	chains					476:481	Spore chains	470:481	Spore chains that differentiated from the vegetative hyphae	470:528	Spore chains that differentiated from the vegetative hyphae contained non-motile rod-shaped spores.
30444473	14	59	dep	sp	2060:2061	arg1	name					2016:2019	the name	2012:2019	the name Allorhizocola rhizosphaerae gen. nov.	2012:2057	The combination of phylogenetic analysis and phenotypic characteristics supported the proposal of strain CPCC 204380T as representing a novel species of a new genus in the family Micromonosporaceae, for which the name Allorhizocola rhizosphaerae gen. nov., sp.
30444473	16	60	theme	species	2154:2160	arg1	strain					2135:2140	the type strain	2126:2140	the type strain of the type species	2126:2160	CPCC 204380T (=DSM 102292T=KCTC 39746 T) is the type strain of the type species.
30444473	16	60	theme	species	2154:2160	arg1	204380T					2087:2093	CPCC 204380T	2082:2093	CPCC 204380T (=DSM 102292T=KCTC 39746 T)	2082:2121	CPCC 204380T (=DSM 102292T=KCTC 39746 T) is the type strain of the type species.
30444473	13	61	theme	DSM	1607:1609	arg1	%					1624:1624	95.9 %	1619:1624	95.9 %	1619:1624	It shared the highest 16S rRNA gene sequence similarities with Rhizocola hellebori K12-0602T (96.1 %), Catellatospora chokoriensis 2-25/1T (95.9 %), Catelliglobosispora koreensis DSM 44566T (95.9 %), Hamadaea tsunoensis DSM 44101T (95.3 %) and Allocatelliglobosispora scoriae Sco-B14 T (94.2 %), and less than 94.0 % sequence similarity with other validly described species.
30444473	13	61	theme	DSM	1607:1609	arg1	44566T					1611:1616	DSM 44566T	1607:1616	Catelliglobosispora koreensis DSM 44566T (95.9 %)	1577:1625	It shared the highest 16S rRNA gene sequence similarities with Rhizocola hellebori K12-0602T (96.1 %), Catellatospora chokoriensis 2-25/1T (95.9 %), Catelliglobosispora koreensis DSM 44566T (95.9 %), Hamadaea tsunoensis DSM 44101T (95.3 %) and Allocatelliglobosispora scoriae Sco-B14 T (94.2 %), and less than 94.0 % sequence similarity with other validly described species.
30444473	5	62	theme	amino	674:678	arg1	acid					618:621	meso-diaminopimelic acid	598:621	meso-diaminopimelic acid	598:621	The peptidoglycan contained meso-diaminopimelic acid and 3-hydroxydiaminopimelic acid as the diagnostic amino acids.
30444473	5	62	theme	amino	674:678	arg1	acids					680:684	the diagnostic amino acids	659:684	the diagnostic amino acids	659:684	The peptidoglycan contained meso-diaminopimelic acid and 3-hydroxydiaminopimelic acid as the diagnostic amino acids.
30444473	5	62	theme	amino	674:678	arg1	acid					651:654	3-hydroxydiaminopimelic acid	627:654	3-hydroxydiaminopimelic acid	627:654	The peptidoglycan contained meso-diaminopimelic acid and 3-hydroxydiaminopimelic acid as the diagnostic amino acids.
30444473	2	63	theme	mongolicum	296:305	arg1	sample					265:270	a rhizosphere soil sample	246:270	a rhizosphere soil sample of the plant Calligonum mongolicum collected from Xinjiang Province, China	246:345	The taxonomic position of an actinobacterium, designated CPCC 204380T, which was isolated from a rhizosphere soil sample of the plant Calligonum mongolicum collected from Xinjiang Province, China, was established using a polyphasic approach.
30444473	16	64	theme	=DSM	2096:2099	arg1	T					2120:2120	=DSM 102292T=KCTC 39746 T	2096:2120	=DSM 102292T=KCTC 39746 T	2096:2120	CPCC 204380T (=DSM 102292T=KCTC 39746 T) is the type strain of the type species.
30444473	16	64	theme	=DSM	2096:2099	arg1	204380T					2087:2093	CPCC 204380T	2082:2093	CPCC 204380T (=DSM 102292T=KCTC 39746 T)	2082:2121	CPCC 204380T (=DSM 102292T=KCTC 39746 T) is the type strain of the type species.
30444473	2	65	theme	plant	279:283	arg1	mongolicum					296:305	the plant Calligonum mongolicum	275:305	the plant Calligonum mongolicum collected from Xinjiang Province, China	275:345	The taxonomic position of an actinobacterium, designated CPCC 204380T, which was isolated from a rhizosphere soil sample of the plant Calligonum mongolicum collected from Xinjiang Province, China, was established using a polyphasic approach.
30444473	14	66	theme	characteristics	1859:1873	arg1	combination					1807:1817	The combination	1803:1817	The combination of phylogenetic analysis and phenotypic characteristics	1803:1873	The combination of phylogenetic analysis and phenotypic characteristics supported the proposal of strain CPCC 204380T as representing a novel species of a new genus in the family Micromonosporaceae, for which the name Allorhizocola rhizosphaerae gen. nov., sp.
30444473	13	67	theme	Sco-B14	1704:1710	arg1	T					1712:1712	Sco-B14 T	1704:1712	Allocatelliglobosispora scoriae Sco-B14 T (94.2 %)	1672:1721	It shared the highest 16S rRNA gene sequence similarities with Rhizocola hellebori K12-0602T (96.1 %), Catellatospora chokoriensis 2-25/1T (95.9 %), Catelliglobosispora koreensis DSM 44566T (95.9 %), Hamadaea tsunoensis DSM 44101T (95.3 %) and Allocatelliglobosispora scoriae Sco-B14 T (94.2 %), and less than 94.0 % sequence similarity with other validly described species.
30444473	13	67	theme	Sco-B14	1704:1710	arg1	%					1720:1720	94.2 %	1715:1720	94.2 %	1715:1720	It shared the highest 16S rRNA gene sequence similarities with Rhizocola hellebori K12-0602T (96.1 %), Catellatospora chokoriensis 2-25/1T (95.9 %), Catelliglobosispora koreensis DSM 44566T (95.9 %), Hamadaea tsunoensis DSM 44101T (95.3 %) and Allocatelliglobosispora scoriae Sco-B14 T (94.2 %), and less than 94.0 % sequence similarity with other validly described species.
30444473	2	68	attach	isolated	232:239	arg1	sample					265:270	a rhizosphere soil sample	246:270	a rhizosphere soil sample of the plant Calligonum mongolicum collected from Xinjiang Province, China	246:345	The taxonomic position of an actinobacterium, designated CPCC 204380T, which was isolated from a rhizosphere soil sample of the plant Calligonum mongolicum collected from Xinjiang Province, China, was established using a polyphasic approach.
30444473	2	68	attach	isolated	232:239	arg2	actinobacterium					180:194	an actinobacterium	177:194	an actinobacterium	177:194	The taxonomic position of an actinobacterium, designated CPCC 204380T, which was isolated from a rhizosphere soil sample of the plant Calligonum mongolicum collected from Xinjiang Province, China, was established using a polyphasic approach.
30444473	10	69	theme	major	1042:1046	arg1	acids					1054:1058	The major fatty acids	1038:1058	The major fatty acids	1038:1058	The major fatty acids were iso-C15 : 0, iso-C16 : 0 and C17 : 1ω9c.
30444473	10	69	theme	major	1042:1046	arg1	 0					1074:1075	 0	1074:1075	 0	1074:1075	The major fatty acids were iso-C15 : 0, iso-C16 : 0 and C17 : 1ω9c.
30444473	10	69	theme	major	1042:1046	arg1	iso-C15 					1065:1072	iso-C15 	1065:1072	iso-C15 	1065:1072	The major fatty acids were iso-C15 : 0, iso-C16 : 0 and C17 : 1ω9c.
30444473	14	70	theme	phylogenetic	1822:1833	arg1	analysis					1835:1842	phylogenetic analysis	1822:1842	phylogenetic analysis	1822:1842	The combination of phylogenetic analysis and phenotypic characteristics supported the proposal of strain CPCC 204380T as representing a novel species of a new genus in the family Micromonosporaceae, for which the name Allorhizocola rhizosphaerae gen. nov., sp.
30444473	14	71	dep	name	2016:2019	arg1	nov.					2054:2057	Allorhizocola rhizosphaerae gen. nov.	2021:2057	the name Allorhizocola rhizosphaerae gen. nov.	2012:2057	The combination of phylogenetic analysis and phenotypic characteristics supported the proposal of strain CPCC 204380T as representing a novel species of a new genus in the family Micromonosporaceae, for which the name Allorhizocola rhizosphaerae gen. nov., sp.
30444473	1	72	attach	isolated	84:91	arg2	Micromonosporaceae					65:82	Micromonosporaceae	65:82	Micromonosporaceae isolated from rhizosphere soil of the plant Calligonum mongolicum	65:148	nov., a new member of Micromonosporaceae isolated from rhizosphere soil of the plant Calligonum mongolicum.
30444473	1	72	attach	isolated	84:91	arg1	soil					110:113	rhizosphere soil	98:113	rhizosphere soil of the plant Calligonum mongolicum	98:148	nov., a new member of Micromonosporaceae isolated from rhizosphere soil of the plant Calligonum mongolicum.
30444473	7	73	located	detected	777:784	arg2	mannose					745:751	mannose	745:751	mannose	745:751	Glucose, mannose, ribose and xylose were detected in whole-cell hydrolysates.
30444473	7	73	located	detected	777:784	arg2	xylose					765:770	xylose	765:770	xylose	765:770	Glucose, mannose, ribose and xylose were detected in whole-cell hydrolysates.
30444473	7	73	located	detected	777:784	arg2	Glucose					736:742	Glucose	736:742	Glucose	736:742	Glucose, mannose, ribose and xylose were detected in whole-cell hydrolysates.
30444473	7	73	located	detected	777:784	arg1	hydrolysates					800:811	whole-cell hydrolysates	789:811	whole-cell hydrolysates	789:811	Glucose, mannose, ribose and xylose were detected in whole-cell hydrolysates.
30444473	7	73	located	detected	777:784	arg2	ribose					754:759	ribose	754:759	ribose	754:759	Glucose, mannose, ribose and xylose were detected in whole-cell hydrolysates.
30444473	12	74	theme	rRNA	1180:1183	arg1	sequences					1190:1198	16S rRNA gene sequences	1176:1198	16S rRNA gene sequences	1176:1198	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CPCC 204380T should be placed in the family Micromonosporaceae, in which it formed a distinct lineage next to the genera Rhizocola, Catellatospora, Catelliglobosispora, Hamadaea and Allocatelliglobosispora.
30444473	1	75	theme	plant	122:126	arg1	mongolicum					139:148	the plant Calligonum mongolicum	118:148	the plant Calligonum mongolicum	118:148	nov., a new member of Micromonosporaceae isolated from rhizosphere soil of the plant Calligonum mongolicum.
30444473	12	76	theme	distinct	1306:1313	arg1	lineage					1315:1321	a distinct lineage	1304:1321	a distinct lineage next to the genera Rhizocola, Catellatospora, Catelliglobosispora, Hamadaea and Allocatelliglobosispora	1304:1425	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CPCC 204380T should be placed in the family Micromonosporaceae, in which it formed a distinct lineage next to the genera Rhizocola, Catellatospora, Catelliglobosispora, Hamadaea and Allocatelliglobosispora.
30444473	1	77	theme	mongolicum	139:148	arg1	soil					110:113	rhizosphere soil	98:113	rhizosphere soil of the plant Calligonum mongolicum	98:148	nov., a new member of Micromonosporaceae isolated from rhizosphere soil of the plant Calligonum mongolicum.
30444473	8	78	theme	predominant	818:828	arg1	menaquinone					830:840	The predominant menaquinone	814:840	The predominant menaquinone	814:840	The predominant menaquinone was MK-10(H8), followed by MK-10(H6) and MK-10(H4).
30444473	8	78	theme	predominant	818:828	arg1	MK-10					846:850	MK-10	846:850	MK-10	846:850	The predominant menaquinone was MK-10(H8), followed by MK-10(H6) and MK-10(H4).
30444473	5	79	theme	3-hydroxydiaminopimelic	627:649	arg1	acid					618:621	meso-diaminopimelic acid	598:621	meso-diaminopimelic acid	598:621	The peptidoglycan contained meso-diaminopimelic acid and 3-hydroxydiaminopimelic acid as the diagnostic amino acids.
30444473	5	79	theme	3-hydroxydiaminopimelic	627:649	arg1	acids					680:684	the diagnostic amino acids	659:684	the diagnostic amino acids	659:684	The peptidoglycan contained meso-diaminopimelic acid and 3-hydroxydiaminopimelic acid as the diagnostic amino acids.
30444473	5	79	theme	3-hydroxydiaminopimelic	627:649	arg1	acid					651:654	3-hydroxydiaminopimelic acid	627:654	3-hydroxydiaminopimelic acid	627:654	The peptidoglycan contained meso-diaminopimelic acid and 3-hydroxydiaminopimelic acid as the diagnostic amino acids.
30444473	1	80	theme	new	51:53	arg1	member					55:60	a new member	49:60	a new member of Micromonosporaceae isolated from rhizosphere soil of the plant Calligonum mongolicum	49:148	nov., a new member of Micromonosporaceae isolated from rhizosphere soil of the plant Calligonum mongolicum.
30444473	1	80	theme	new	51:53	arg1	nov.					43:46	nov.	43:46	nov.	43:46	nov., a new member of Micromonosporaceae isolated from rhizosphere soil of the plant Calligonum mongolicum.
30444473	16	81	theme	39746	2114:2118	arg1	T					2120:2120	=DSM 102292T=KCTC 39746 T	2096:2120	=DSM 102292T=KCTC 39746 T	2096:2120	CPCC 204380T (=DSM 102292T=KCTC 39746 T) is the type strain of the type species.
30444473	16	81	theme	39746	2114:2118	arg1	204380T					2087:2093	CPCC 204380T	2082:2093	CPCC 204380T (=DSM 102292T=KCTC 39746 T)	2082:2121	CPCC 204380T (=DSM 102292T=KCTC 39746 T) is the type strain of the type species.
30444473	3	82	theme	globose	430:436	arg1	bodies					438:443	well and globose bodies	421:443	bodies	438:443	Vegetative hyphae developed well and globose bodies formed from aged hyphae.
30444473	0	83	theme	rhizosphaerae	14:26	arg1	nov.					33:36	Allorhizocola rhizosphaerae gen. nov.	0:36	Allorhizocola rhizosphaerae gen. nov.	0:36	Allorhizocola rhizosphaerae gen. nov., sp.
30444473	14	84	theme	genus	1962:1966	arg1	species					1945:1951	a novel species	1937:1951	a novel species	1937:1951	The combination of phylogenetic analysis and phenotypic characteristics supported the proposal of strain CPCC 204380T as representing a novel species of a new genus in the family Micromonosporaceae, for which the name Allorhizocola rhizosphaerae gen. nov., sp.
30444473	3	85	theme	well	421:424	arg1	bodies					438:443	well and globose bodies	421:443	bodies	438:443	Vegetative hyphae developed well and globose bodies formed from aged hyphae.
30444473	14	86	theme	rhizosphaerae	2035:2047	arg1	nov.					2054:2057	Allorhizocola rhizosphaerae gen. nov.	2021:2057	the name Allorhizocola rhizosphaerae gen. nov.	2012:2057	The combination of phylogenetic analysis and phenotypic characteristics supported the proposal of strain CPCC 204380T as representing a novel species of a new genus in the family Micromonosporaceae, for which the name Allorhizocola rhizosphaerae gen. nov., sp.
30444473	13	87	theme	sequence	1464:1471	arg1	similarities					1473:1484	the highest 16S rRNA gene sequence similarities	1438:1484	the highest 16S rRNA gene sequence similarities	1438:1484	It shared the highest 16S rRNA gene sequence similarities with Rhizocola hellebori K12-0602T (96.1 %), Catellatospora chokoriensis 2-25/1T (95.9 %), Catelliglobosispora koreensis DSM 44566T (95.9 %), Hamadaea tsunoensis DSM 44101T (95.3 %) and Allocatelliglobosispora scoriae Sco-B14 T (94.2 %), and less than 94.0 % sequence similarity with other validly described species.
30444473	11	88	theme	G+C	1118:1120	arg1	content					1122:1128	The genomic G+C content	1106:1128	The genomic G+C content	1106:1128	The genomic G+C content was 64.9 mol%.
30444473	11	88	theme	G+C	1118:1120	arg1	%					1142:1142	64.9 mol%	1134:1142	64.9 mol%	1134:1142	The genomic G+C content was 64.9 mol%.
30444473	13	89	theme	DSM	1648:1650	arg1	44101T					1652:1657	DSM 44101T	1648:1657	Hamadaea tsunoensis DSM 44101T (95.3 %)	1628:1666	It shared the highest 16S rRNA gene sequence similarities with Rhizocola hellebori K12-0602T (96.1 %), Catellatospora chokoriensis 2-25/1T (95.9 %), Catelliglobosispora koreensis DSM 44566T (95.9 %), Hamadaea tsunoensis DSM 44101T (95.3 %) and Allocatelliglobosispora scoriae Sco-B14 T (94.2 %), and less than 94.0 % sequence similarity with other validly described species.
30444473	13	89	theme	DSM	1648:1650	arg1	%					1665:1665	95.3 %	1660:1665	95.3 %	1660:1665	It shared the highest 16S rRNA gene sequence similarities with Rhizocola hellebori K12-0602T (96.1 %), Catellatospora chokoriensis 2-25/1T (95.9 %), Catelliglobosispora koreensis DSM 44566T (95.9 %), Hamadaea tsunoensis DSM 44101T (95.3 %) and Allocatelliglobosispora scoriae Sco-B14 T (94.2 %), and less than 94.0 % sequence similarity with other validly described species.
30444473	2	90	theme	taxonomic	155:163	arg1	position					165:172	The taxonomic position	151:172	The taxonomic position of an actinobacterium, designated CPCC 204380T, which was isolated from a rhizosphere soil sample of the plant Calligonum mongolicum collected from Xinjiang Province, China,	151:346	The taxonomic position of an actinobacterium, designated CPCC 204380T, which was isolated from a rhizosphere soil sample of the plant Calligonum mongolicum collected from Xinjiang Province, China, was established using a polyphasic approach.
30444473	9	91	dep	diphosphatidylglycerol	931:952	arg1	mannoside					1027:1035	mannoside	1027:1035	mannoside	1027:1035	The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside.
30444473	0	92	dep	sp	39:40	arg1	nov.					33:36	Allorhizocola rhizosphaerae gen. nov.	0:36	Allorhizocola rhizosphaerae gen. nov.	0:36	Allorhizocola rhizosphaerae gen. nov., sp.
30444473	4	93	theme	non-motile	540:549	arg1	spores					562:567	non-motile rod-shaped spores	540:567	non-motile rod-shaped spores	540:567	Spore chains that differentiated from the vegetative hyphae contained non-motile rod-shaped spores.
30444473	13	94	theme	highest	1442:1448	arg1	rRNA					1454:1457	the highest 16S rRNA	1438:1457	the highest 16S rRNA gene sequence similarities	1438:1484	It shared the highest 16S rRNA gene sequence similarities with Rhizocola hellebori K12-0602T (96.1 %), Catellatospora chokoriensis 2-25/1T (95.9 %), Catelliglobosispora koreensis DSM 44566T (95.9 %), Hamadaea tsunoensis DSM 44101T (95.3 %) and Allocatelliglobosispora scoriae Sco-B14 T (94.2 %), and less than 94.0 % sequence similarity with other validly described species.
30444473	11	95	theme	64.9 mol	1134:1141	arg1	content					1122:1128	The genomic G+C content	1106:1128	The genomic G+C content	1106:1128	The genomic G+C content was 64.9 mol%.
30444473	11	95	theme	64.9 mol	1134:1141	arg1	%					1142:1142	64.9 mol%	1134:1142	64.9 mol%	1134:1142	The genomic G+C content was 64.9 mol%.
30444473	13	96	dep	Catelliglobosispora	1577:1595	arg1	%					1624:1624	95.9 %	1619:1624	95.9 %	1619:1624	It shared the highest 16S rRNA gene sequence similarities with Rhizocola hellebori K12-0602T (96.1 %), Catellatospora chokoriensis 2-25/1T (95.9 %), Catelliglobosispora koreensis DSM 44566T (95.9 %), Hamadaea tsunoensis DSM 44101T (95.3 %) and Allocatelliglobosispora scoriae Sco-B14 T (94.2 %), and less than 94.0 % sequence similarity with other validly described species.
30444473	13	96	dep	Catelliglobosispora	1577:1595	arg1	koreensis					1597:1605	Catelliglobosispora koreensis DSM 44566T (95.9 %)	1577:1625	Catelliglobosispora koreensis DSM 44566T (95.9 %)	1577:1625	It shared the highest 16S rRNA gene sequence similarities with Rhizocola hellebori K12-0602T (96.1 %), Catellatospora chokoriensis 2-25/1T (95.9 %), Catelliglobosispora koreensis DSM 44566T (95.9 %), Hamadaea tsunoensis DSM 44101T (95.3 %) and Allocatelliglobosispora scoriae Sco-B14 T (94.2 %), and less than 94.0 % sequence similarity with other validly described species.
30444473	13	96	dep	Catelliglobosispora	1577:1595	arg1	44566T					1611:1616	DSM 44566T	1607:1616	Catelliglobosispora koreensis DSM 44566T (95.9 %)	1577:1625	It shared the highest 16S rRNA gene sequence similarities with Rhizocola hellebori K12-0602T (96.1 %), Catellatospora chokoriensis 2-25/1T (95.9 %), Catelliglobosispora koreensis DSM 44566T (95.9 %), Hamadaea tsunoensis DSM 44101T (95.3 %) and Allocatelliglobosispora scoriae Sco-B14 T (94.2 %), and less than 94.0 % sequence similarity with other validly described species.
30444473	2	97	theme	actinobacterium	180:194	arg1	position					165:172	The taxonomic position	151:172	The taxonomic position of an actinobacterium, designated CPCC 204380T, which was isolated from a rhizosphere soil sample of the plant Calligonum mongolicum collected from Xinjiang Province, China,	151:346	The taxonomic position of an actinobacterium, designated CPCC 204380T, which was isolated from a rhizosphere soil sample of the plant Calligonum mongolicum collected from Xinjiang Province, China, was established using a polyphasic approach.
30444473	13	98	theme	rRNA	1454:1457	arg1	similarities					1473:1484	the highest 16S rRNA gene sequence similarities	1438:1484	the highest 16S rRNA gene sequence similarities	1438:1484	It shared the highest 16S rRNA gene sequence similarities with Rhizocola hellebori K12-0602T (96.1 %), Catellatospora chokoriensis 2-25/1T (95.9 %), Catelliglobosispora koreensis DSM 44566T (95.9 %), Hamadaea tsunoensis DSM 44101T (95.3 %) and Allocatelliglobosispora scoriae Sco-B14 T (94.2 %), and less than 94.0 % sequence similarity with other validly described species.
30444473	14	99	theme	CPCC	1908:1911	arg1	204380T					1913:1919	strain CPCC 204380T	1901:1919	strain CPCC 204380T	1901:1919	The combination of phylogenetic analysis and phenotypic characteristics supported the proposal of strain CPCC 204380T as representing a novel species of a new genus in the family Micromonosporaceae, for which the name Allorhizocola rhizosphaerae gen. nov., sp.
30444473	9	100	theme	polar	898:902	arg1	profile					910:916	The polar lipid profile	894:916	The polar lipid profile	894:916	The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside.
29458462	9	0	theme	gene	869:872	arg1	similarity					883:892	97.8, 97.6 and 97.0 % 16S rRNA gene sequence similarity	838:892	97.8, 97.6 and 97.0 % 16S rRNA gene sequence similarity	838:892	Strain Bb 2-3T was closely related to Lactobacillus bombicola DSM 28793T, Lactobacillus apis LMG 26964T and Lactobacillus helsingborgensis DSM 26265T, with 97.8, 97.6 and 97.0 % 16S rRNA gene sequence similarity, respectively.
29458462	9	1	theme	helsingborgensis	804:819	arg1	26265T					825:830	Lactobacillus helsingborgensis DSM 26265T	790:830	Lactobacillus helsingborgensis DSM 26265T	790:830	Strain Bb 2-3T was closely related to Lactobacillus bombicola DSM 28793T, Lactobacillus apis LMG 26964T and Lactobacillus helsingborgensis DSM 26265T, with 97.8, 97.6 and 97.0 % 16S rRNA gene sequence similarity, respectively.
29458462	15	2	theme	Bb	1577:1578	arg1	19955T					1604:1609	=DSM 102188T=ACCC 19955T	1586:1609	=DSM 102188T=ACCC 19955T	1586:1609	The type strain is Bb 2-3T (=DSM 102188T=ACCC 19955T).
29458462	15	2	theme	Bb	1577:1578	arg1	strain					1567:1572	The type strain	1558:1572	The type strain	1558:1572	The type strain is Bb 2-3T (=DSM 102188T=ACCC 19955T).
29458462	15	2	theme	Bb	1577:1578	arg1	2-3T					1580:1583	Bb 2-3T	1577:1583	Bb 2-3T (=DSM 102188T=ACCC 19955T)	1577:1610	The type strain is Bb 2-3T (=DSM 102188T=ACCC 19955T).
29458462	12	3	theme	strain	1310:1315	arg1	2-3T					1320:1323	strain Bb 2-3T	1310:1323	strain Bb 2-3T	1310:1323	The G+C content of the genomic DNA of strain Bb 2-3T was 37.4 mol%.
29458462	8	4	theme	peptidoglycan	631:643	arg1	l-Lys-d-Asp					654:664	The peptidoglycan type A4α l-Lys-d-Asp	627:664	The peptidoglycan type A4α l-Lys-d-Asp	627:664	The peptidoglycan type A4α l-Lys-d-Asp was determined.
29458462	2	5	theme	Apis	271:274	arg1	cerana					276:281	Apis cerana	271:281	Apis cerana collected from a hive in Kunming, China	271:321	A novel facultatively anaerobic, Gram-stain-positive, non-motile, non-spore-forming, catalase-negative bacterium of the genus Lactobacillus, designated strain Bb 2-3T, was isolated from bee bread of Apis cerana collected from a hive in Kunming, China.
29458462	12	6	theme	2-3T	1320:1323	arg1	DNA					1303:1305	the genomic DNA	1291:1305	the genomic DNA of strain Bb 2-3T	1291:1323	The G+C content of the genomic DNA of strain Bb 2-3T was 37.4 mol%.
29458462	8	7	theme	A4α	650:652	arg1	l-Lys-d-Asp					654:664	The peptidoglycan type A4α l-Lys-d-Asp	627:664	The peptidoglycan type A4α l-Lys-d-Asp	627:664	The peptidoglycan type A4α l-Lys-d-Asp was determined.
29458462	9	8	theme	Strain	682:687	arg1	2-3T					692:695	Strain Bb 2-3T	682:695	Strain Bb 2-3T	682:695	Strain Bb 2-3T was closely related to Lactobacillus bombicola DSM 28793T, Lactobacillus apis LMG 26964T and Lactobacillus helsingborgensis DSM 26265T, with 97.8, 97.6 and 97.0 % 16S rRNA gene sequence similarity, respectively.
29458462	11	9	theme	prokaryotic	1251:1261	arg1	species					1263:1269	genomic prokaryotic species	1243:1269	genomic prokaryotic species	1243:1269	The average nucleotide identity between strain Bb 2-3T and the type strains of closely related species was lower than the 95-96 % threshold value for delineation of genomic prokaryotic species.
29458462	9	10	theme	DSM	821:823	arg1	26265T					825:830	Lactobacillus helsingborgensis DSM 26265T	790:830	Lactobacillus helsingborgensis DSM 26265T	790:830	Strain Bb 2-3T was closely related to Lactobacillus bombicola DSM 28793T, Lactobacillus apis LMG 26964T and Lactobacillus helsingborgensis DSM 26265T, with 97.8, 97.6 and 97.0 % 16S rRNA gene sequence similarity, respectively.
29458462	2	11	theme	strain	224:229	arg1	2-3T					234:237	designated strain Bb 2-3T	213:237	designated strain Bb 2-3T	213:237	A novel facultatively anaerobic, Gram-stain-positive, non-motile, non-spore-forming, catalase-negative bacterium of the genus Lactobacillus, designated strain Bb 2-3T, was isolated from bee bread of Apis cerana collected from a hive in Kunming, China.
29458462	2	11	theme	strain	224:229	arg1	bacterium					175:183	A novel facultatively anaerobic, Gram-stain-positive, non-motile, non-spore-forming, catalase-negative bacterium	72:183	A novel facultatively anaerobic, Gram-stain-positive, non-motile, non-spore-forming, catalase-negative bacterium of the genus Lactobacillus	72:210	A novel facultatively anaerobic, Gram-stain-positive, non-motile, non-spore-forming, catalase-negative bacterium of the genus Lactobacillus, designated strain Bb 2-3T, was isolated from bee bread of Apis cerana collected from a hive in Kunming, China.
29458462	12	12	theme	DNA	1303:1305	arg1	%					1337:1337	37.4 mol%	1329:1337	37.4 mol%	1329:1337	The G+C content of the genomic DNA of strain Bb 2-3T was 37.4 mol%.
29458462	12	12	theme	DNA	1303:1305	arg1	G+C content					1276:1286	The G+C content	1272:1286	The G+C content of the genomic DNA of strain Bb 2-3T	1272:1323	The G+C content of the genomic DNA of strain Bb 2-3T was 37.4 mol%.
29458462	13	13	theme	genus	1473:1477	arg1	Lactobacillus					1479:1491	the genus Lactobacillus	1469:1491	the genus Lactobacillus	1469:1491	On the basis of phenotypic, chemotaxonomic and phylogenetic analyses, strain Bb 2-3T is proposed to represent a novel species of the genus Lactobacillus, for which we propose the name Lactobacillus panisapium sp.
29458462	5	14	theme	fatty	437:441	arg1	C18 					454:457	C18 	454:457	C18 	454:457	The predominant fatty acids were C18 : 1ω9c, C16 : 0 and C19 : 0 iso.
29458462	5	14	theme	fatty	437:441	arg1	acids					443:447	The predominant fatty acids	421:447	The predominant fatty acids	421:447	The predominant fatty acids were C18 : 1ω9c, C16 : 0 and C19 : 0 iso.
29458462	4	15	theme	5.0 g	405:409	arg1	l-1					411:413	l-1	411:413	l-1	411:413	Optimal growth occurred at 37 °C, pH 6.5 with 5.0 g l-1 NaCl.
29458462	1	16	dep	nov.	29:32	arg1	bread					65:69	honeybee Apis cerana bee bread	40:69	honeybee Apis cerana bee bread	40:69	nov., from honeybee Apis cerana bee bread.
29458462	6	17	theme	Respiratory	491:501	arg1	quinones					503:510	Respiratory quinones	491:510	Respiratory quinones	491:510	Respiratory quinones were not detected.
29458462	10	18	theme	Bb	985:986	arg1	2-3T					988:991	strain Bb 2-3T	978:991	strain Bb 2-3T	978:991	A comparison of two housekeeping genes, rpoA and pheS, revealed that strain Bb 2-3T was well separated from the reference strains of species of the genus Lactobacillus.
29458462	13	19	theme	novel	1452:1456	arg1	species					1458:1464	a novel species	1450:1464	a novel species	1450:1464	On the basis of phenotypic, chemotaxonomic and phylogenetic analyses, strain Bb 2-3T is proposed to represent a novel species of the genus Lactobacillus, for which we propose the name Lactobacillus panisapium sp.
29458462	2	20	theme	bee	258:260	arg1	bread					262:266	bee bread	258:266	bee bread of Apis cerana collected from a hive in Kunming, China	258:321	A novel facultatively anaerobic, Gram-stain-positive, non-motile, non-spore-forming, catalase-negative bacterium of the genus Lactobacillus, designated strain Bb 2-3T, was isolated from bee bread of Apis cerana collected from a hive in Kunming, China.
29458462	13	21	theme	Lactobacillus	1479:1491	arg1	species					1458:1464	a novel species	1450:1464	a novel species	1450:1464	On the basis of phenotypic, chemotaxonomic and phylogenetic analyses, strain Bb 2-3T is proposed to represent a novel species of the genus Lactobacillus, for which we propose the name Lactobacillus panisapium sp.
29458462	2	22	attach	isolated	244:251	arg1	bread					262:266	bee bread	258:266	bee bread of Apis cerana collected from a hive in Kunming, China	258:321	A novel facultatively anaerobic, Gram-stain-positive, non-motile, non-spore-forming, catalase-negative bacterium of the genus Lactobacillus, designated strain Bb 2-3T, was isolated from bee bread of Apis cerana collected from a hive in Kunming, China.
29458462	2	22	attach	isolated	244:251	arg2	bacterium					175:183	A novel facultatively anaerobic, Gram-stain-positive, non-motile, non-spore-forming, catalase-negative bacterium	72:183	A novel facultatively anaerobic, Gram-stain-positive, non-motile, non-spore-forming, catalase-negative bacterium of the genus Lactobacillus	72:210	A novel facultatively anaerobic, Gram-stain-positive, non-motile, non-spore-forming, catalase-negative bacterium of the genus Lactobacillus, designated strain Bb 2-3T, was isolated from bee bread of Apis cerana collected from a hive in Kunming, China.
29458462	2	22	attach	isolated	244:251	arg2	2-3T					234:237	designated strain Bb 2-3T	213:237	designated strain Bb 2-3T	213:237	A novel facultatively anaerobic, Gram-stain-positive, non-motile, non-spore-forming, catalase-negative bacterium of the genus Lactobacillus, designated strain Bb 2-3T, was isolated from bee bread of Apis cerana collected from a hive in Kunming, China.
29458462	9	23	theme	Lactobacillus	756:768	arg1	26964T					779:784	Lactobacillus apis LMG 26964T	756:784	Lactobacillus apis LMG 26964T	756:784	Strain Bb 2-3T was closely related to Lactobacillus bombicola DSM 28793T, Lactobacillus apis LMG 26964T and Lactobacillus helsingborgensis DSM 26265T, with 97.8, 97.6 and 97.0 % 16S rRNA gene sequence similarity, respectively.
29458462	2	24	from	hive	300:303	arg1	China					317:321	China	317:321	China	317:321	A novel facultatively anaerobic, Gram-stain-positive, non-motile, non-spore-forming, catalase-negative bacterium of the genus Lactobacillus, designated strain Bb 2-3T, was isolated from bee bread of Apis cerana collected from a hive in Kunming, China.
29458462	9	25	theme	LMG	775:777	arg1	26964T					779:784	Lactobacillus apis LMG 26964T	756:784	Lactobacillus apis LMG 26964T	756:784	Strain Bb 2-3T was closely related to Lactobacillus bombicola DSM 28793T, Lactobacillus apis LMG 26964T and Lactobacillus helsingborgensis DSM 26265T, with 97.8, 97.6 and 97.0 % 16S rRNA gene sequence similarity, respectively.
29458462	15	26	theme	=DSM	1586:1589	arg1	19955T					1604:1609	=DSM 102188T=ACCC 19955T	1586:1609	=DSM 102188T=ACCC 19955T	1586:1609	The type strain is Bb 2-3T (=DSM 102188T=ACCC 19955T).
29458462	15	26	theme	=DSM	1586:1589	arg1	2-3T					1580:1583	Bb 2-3T	1577:1583	Bb 2-3T (=DSM 102188T=ACCC 19955T)	1577:1610	The type strain is Bb 2-3T (=DSM 102188T=ACCC 19955T).
29458462	1	27	theme	cerana	54:59	arg1	bread					65:69	honeybee Apis cerana bee bread	40:69	honeybee Apis cerana bee bread	40:69	nov., from honeybee Apis cerana bee bread.
29458462	13	28	theme	phylogenetic	1387:1398	arg1	analyses					1400:1407	phenotypic, chemotaxonomic and phylogenetic analyses	1356:1407	phenotypic, chemotaxonomic and phylogenetic analyses	1356:1407	On the basis of phenotypic, chemotaxonomic and phylogenetic analyses, strain Bb 2-3T is proposed to represent a novel species of the genus Lactobacillus, for which we propose the name Lactobacillus panisapium sp.
29458462	9	29	with	related	709:715	arg1	similarity					883:892	97.8, 97.6 and 97.0 % 16S rRNA gene sequence similarity	838:892	97.8, 97.6 and 97.0 % 16S rRNA gene sequence similarity	838:892	Strain Bb 2-3T was closely related to Lactobacillus bombicola DSM 28793T, Lactobacillus apis LMG 26964T and Lactobacillus helsingborgensis DSM 26265T, with 97.8, 97.6 and 97.0 % 16S rRNA gene sequence similarity, respectively.
29458462	11	30	theme	nucleotide	1090:1099	arg1	identity					1101:1108	The average nucleotide identity	1078:1108	The average nucleotide identity between strain Bb 2-3T and the type strains of closely related species	1078:1179	The average nucleotide identity between strain Bb 2-3T and the type strains of closely related species was lower than the 95-96 % threshold value for delineation of genomic prokaryotic species.
29458462	11	30	theme	nucleotide	1090:1099	arg1	lower					1185:1189	lower	1185:1189	lower	1185:1189	The average nucleotide identity between strain Bb 2-3T and the type strains of closely related species was lower than the 95-96 % threshold value for delineation of genomic prokaryotic species.
29458462	11	31	theme	%	1206:1206	arg1	value					1218:1222	the 95-96 % threshold value	1196:1222	the 95-96 % threshold value for delineation of genomic prokaryotic species	1196:1269	The average nucleotide identity between strain Bb 2-3T and the type strains of closely related species was lower than the 95-96 % threshold value for delineation of genomic prokaryotic species.
29458462	0	32	theme	Lactobacillus	0:12	arg1	sp					25:26	Lactobacillus panisapium sp	0:26	Lactobacillus panisapium sp.	0:27	Lactobacillus panisapium sp.
29458462	13	33	theme	Lactobacillus	1524:1536	arg1	sp					1549:1550	the name Lactobacillus panisapium sp	1515:1550	the name Lactobacillus panisapium sp	1515:1550	On the basis of phenotypic, chemotaxonomic and phylogenetic analyses, strain Bb 2-3T is proposed to represent a novel species of the genus Lactobacillus, for which we propose the name Lactobacillus panisapium sp.
29458462	13	34	theme	phenotypic	1356:1365	arg1	analyses					1400:1407	phenotypic, chemotaxonomic and phylogenetic analyses	1356:1407	phenotypic, chemotaxonomic and phylogenetic analyses	1356:1407	On the basis of phenotypic, chemotaxonomic and phylogenetic analyses, strain Bb 2-3T is proposed to represent a novel species of the genus Lactobacillus, for which we propose the name Lactobacillus panisapium sp.
29458462	10	35	theme	species	1042:1048	arg1	strains					1031:1037	the reference strains	1017:1037	the reference strains of species of the genus Lactobacillus	1017:1075	A comparison of two housekeeping genes, rpoA and pheS, revealed that strain Bb 2-3T was well separated from the reference strains of species of the genus Lactobacillus.
29458462	11	36	theme	Bb	1125:1126	arg1	2-3T					1128:1131	strain Bb 2-3T	1118:1131	strain Bb 2-3T	1118:1131	The average nucleotide identity between strain Bb 2-3T and the type strains of closely related species was lower than the 95-96 % threshold value for delineation of genomic prokaryotic species.
29458462	13	37	theme	chemotaxonomic	1368:1381	arg1	analyses					1400:1407	phenotypic, chemotaxonomic and phylogenetic analyses	1356:1407	phenotypic, chemotaxonomic and phylogenetic analyses	1356:1407	On the basis of phenotypic, chemotaxonomic and phylogenetic analyses, strain Bb 2-3T is proposed to represent a novel species of the genus Lactobacillus, for which we propose the name Lactobacillus panisapium sp.
29458462	2	38	dep	anaerobic	94:102	arg1	Gram-stain-positive					105:123	Gram-stain-positive	105:123	Gram-stain-positive	105:123	A novel facultatively anaerobic, Gram-stain-positive, non-motile, non-spore-forming, catalase-negative bacterium of the genus Lactobacillus, designated strain Bb 2-3T, was isolated from bee bread of Apis cerana collected from a hive in Kunming, China.
29458462	2	38	dep	anaerobic	94:102	arg1	catalase-negative					157:173	catalase-negative	157:173	catalase-negative	157:173	A novel facultatively anaerobic, Gram-stain-positive, non-motile, non-spore-forming, catalase-negative bacterium of the genus Lactobacillus, designated strain Bb 2-3T, was isolated from bee bread of Apis cerana collected from a hive in Kunming, China.
29458462	2	38	dep	anaerobic	94:102	arg1	non-spore-forming					138:154	non-spore-forming	138:154	non-spore-forming	138:154	A novel facultatively anaerobic, Gram-stain-positive, non-motile, non-spore-forming, catalase-negative bacterium of the genus Lactobacillus, designated strain Bb 2-3T, was isolated from bee bread of Apis cerana collected from a hive in Kunming, China.
29458462	2	38	dep	anaerobic	94:102	arg1	non-motile					126:135	non-motile	126:135	non-motile	126:135	A novel facultatively anaerobic, Gram-stain-positive, non-motile, non-spore-forming, catalase-negative bacterium of the genus Lactobacillus, designated strain Bb 2-3T, was isolated from bee bread of Apis cerana collected from a hive in Kunming, China.
29458462	11	39	theme	species	1173:1179	arg1	strains					1146:1152	the type strains	1137:1152	the type strains of closely related species	1137:1179	The average nucleotide identity between strain Bb 2-3T and the type strains of closely related species was lower than the 95-96 % threshold value for delineation of genomic prokaryotic species.
29458462	11	39	theme	species	1173:1179	arg1	2-3T					1128:1131	strain Bb 2-3T	1118:1131	strain Bb 2-3T	1118:1131	The average nucleotide identity between strain Bb 2-3T and the type strains of closely related species was lower than the 95-96 % threshold value for delineation of genomic prokaryotic species.
29458462	10	40	theme	Lactobacillus	1063:1075	arg1	species					1042:1048	species	1042:1048	species of the genus Lactobacillus	1042:1075	A comparison of two housekeeping genes, rpoA and pheS, revealed that strain Bb 2-3T was well separated from the reference strains of species of the genus Lactobacillus.
29458462	9	41	dep	similarity	883:892	arg1	%					858:858	%	858:858	%	858:858	Strain Bb 2-3T was closely related to Lactobacillus bombicola DSM 28793T, Lactobacillus apis LMG 26964T and Lactobacillus helsingborgensis DSM 26265T, with 97.8, 97.6 and 97.0 % 16S rRNA gene sequence similarity, respectively.
29458462	10	42	theme	genes	942:946	arg1	comparison					911:920	A comparison	909:920	A comparison of two housekeeping genes, rpoA and pheS,	909:962	A comparison of two housekeeping genes, rpoA and pheS, revealed that strain Bb 2-3T was well separated from the reference strains of species of the genus Lactobacillus.
29458462	2	43	theme	genus	192:196	arg1	Lactobacillus					198:210	the genus Lactobacillus	188:210	the genus Lactobacillus	188:210	A novel facultatively anaerobic, Gram-stain-positive, non-motile, non-spore-forming, catalase-negative bacterium of the genus Lactobacillus, designated strain Bb 2-3T, was isolated from bee bread of Apis cerana collected from a hive in Kunming, China.
29458462	1	44	theme	honeybee	40:47	arg1	bread					65:69	honeybee Apis cerana bee bread	40:69	honeybee Apis cerana bee bread	40:69	nov., from honeybee Apis cerana bee bread.
29458462	9	45	theme	rRNA	864:867	arg1	similarity					883:892	97.8, 97.6 and 97.0 % 16S rRNA gene sequence similarity	838:892	97.8, 97.6 and 97.0 % 16S rRNA gene sequence similarity	838:892	Strain Bb 2-3T was closely related to Lactobacillus bombicola DSM 28793T, Lactobacillus apis LMG 26964T and Lactobacillus helsingborgensis DSM 26265T, with 97.8, 97.6 and 97.0 % 16S rRNA gene sequence similarity, respectively.
29458462	9	46	theme	sequence	874:881	arg1	similarity					883:892	97.8, 97.6 and 97.0 % 16S rRNA gene sequence similarity	838:892	97.8, 97.6 and 97.0 % 16S rRNA gene sequence similarity	838:892	Strain Bb 2-3T was closely related to Lactobacillus bombicola DSM 28793T, Lactobacillus apis LMG 26964T and Lactobacillus helsingborgensis DSM 26265T, with 97.8, 97.6 and 97.0 % 16S rRNA gene sequence similarity, respectively.
29458462	2	47	theme	anaerobic	94:102	arg1	2-3T					234:237	designated strain Bb 2-3T	213:237	designated strain Bb 2-3T	213:237	A novel facultatively anaerobic, Gram-stain-positive, non-motile, non-spore-forming, catalase-negative bacterium of the genus Lactobacillus, designated strain Bb 2-3T, was isolated from bee bread of Apis cerana collected from a hive in Kunming, China.
29458462	2	47	theme	anaerobic	94:102	arg1	bacterium					175:183	A novel facultatively anaerobic, Gram-stain-positive, non-motile, non-spore-forming, catalase-negative bacterium	72:183	A novel facultatively anaerobic, Gram-stain-positive, non-motile, non-spore-forming, catalase-negative bacterium of the genus Lactobacillus	72:210	A novel facultatively anaerobic, Gram-stain-positive, non-motile, non-spore-forming, catalase-negative bacterium of the genus Lactobacillus, designated strain Bb 2-3T, was isolated from bee bread of Apis cerana collected from a hive in Kunming, China.
29458462	2	48	theme	cerana	276:281	arg1	bread					262:266	bee bread	258:266	bee bread of Apis cerana collected from a hive in Kunming, China	258:321	A novel facultatively anaerobic, Gram-stain-positive, non-motile, non-spore-forming, catalase-negative bacterium of the genus Lactobacillus, designated strain Bb 2-3T, was isolated from bee bread of Apis cerana collected from a hive in Kunming, China.
29458462	12	49	theme	37.4 mol	1329:1336	arg1	%					1337:1337	37.4 mol%	1329:1337	37.4 mol%	1329:1337	The G+C content of the genomic DNA of strain Bb 2-3T was 37.4 mol%.
29458462	12	49	theme	37.4 mol	1329:1336	arg1	G+C content					1276:1286	The G+C content	1272:1286	The G+C content of the genomic DNA of strain Bb 2-3T	1272:1323	The G+C content of the genomic DNA of strain Bb 2-3T was 37.4 mol%.
29458462	13	50	dep	analyses	1400:1407	arg1	the					1343:1345	the	1343:1345	the	1343:1345	On the basis of phenotypic, chemotaxonomic and phylogenetic analyses, strain Bb 2-3T is proposed to represent a novel species of the genus Lactobacillus, for which we propose the name Lactobacillus panisapium sp.
29458462	13	50	dep	analyses	1400:1407	arg1	basis					1347:1351	basis	1347:1351	basis	1347:1351	On the basis of phenotypic, chemotaxonomic and phylogenetic analyses, strain Bb 2-3T is proposed to represent a novel species of the genus Lactobacillus, for which we propose the name Lactobacillus panisapium sp.
29458462	12	51	theme	Bb	1317:1318	arg1	2-3T					1320:1323	strain Bb 2-3T	1310:1323	strain Bb 2-3T	1310:1323	The G+C content of the genomic DNA of strain Bb 2-3T was 37.4 mol%.
29458462	11	52	theme	genomic	1243:1249	arg1	species					1263:1269	genomic prokaryotic species	1243:1269	genomic prokaryotic species	1243:1269	The average nucleotide identity between strain Bb 2-3T and the type strains of closely related species was lower than the 95-96 % threshold value for delineation of genomic prokaryotic species.
29458462	8	53	theme	type	645:648	arg1	l-Lys-d-Asp					654:664	The peptidoglycan type A4α l-Lys-d-Asp	627:664	The peptidoglycan type A4α l-Lys-d-Asp	627:664	The peptidoglycan type A4α l-Lys-d-Asp was determined.
29458462	15	54	theme	type	1562:1565	arg1	strain					1567:1572	The type strain	1558:1572	The type strain	1558:1572	The type strain is Bb 2-3T (=DSM 102188T=ACCC 19955T).
29458462	15	54	theme	type	1562:1565	arg1	2-3T					1580:1583	Bb 2-3T	1577:1583	Bb 2-3T (=DSM 102188T=ACCC 19955T)	1577:1610	The type strain is Bb 2-3T (=DSM 102188T=ACCC 19955T).
29458462	4	55	theme	Optimal	359:365	arg1	growth					367:372	Optimal growth	359:372	Optimal growth	359:372	Optimal growth occurred at 37 °C, pH 6.5 with 5.0 g l-1 NaCl.
29458462	9	56	theme	Bb	689:690	arg1	2-3T					692:695	Strain Bb 2-3T	682:695	Strain Bb 2-3T	682:695	Strain Bb 2-3T was closely related to Lactobacillus bombicola DSM 28793T, Lactobacillus apis LMG 26964T and Lactobacillus helsingborgensis DSM 26265T, with 97.8, 97.6 and 97.0 % 16S rRNA gene sequence similarity, respectively.
29458462	11	57	theme	95-96 	1200:1205	arg1	%					1206:1206	%	1206:1206	%	1206:1206	The average nucleotide identity between strain Bb 2-3T and the type strains of closely related species was lower than the 95-96 % threshold value for delineation of genomic prokaryotic species.
29458462	5	58	theme	predominant	425:435	arg1	C18 					454:457	C18 	454:457	C18 	454:457	The predominant fatty acids were C18 : 1ω9c, C16 : 0 and C19 : 0 iso.
29458462	5	58	theme	predominant	425:435	arg1	acids					443:447	The predominant fatty acids	421:447	The predominant fatty acids	421:447	The predominant fatty acids were C18 : 1ω9c, C16 : 0 and C19 : 0 iso.
29458462	11	59	theme	average	1082:1088	arg1	identity					1101:1108	The average nucleotide identity	1078:1108	The average nucleotide identity between strain Bb 2-3T and the type strains of closely related species	1078:1179	The average nucleotide identity between strain Bb 2-3T and the type strains of closely related species was lower than the 95-96 % threshold value for delineation of genomic prokaryotic species.
29458462	11	59	theme	average	1082:1088	arg1	lower					1185:1189	lower	1185:1189	lower	1185:1189	The average nucleotide identity between strain Bb 2-3T and the type strains of closely related species was lower than the 95-96 % threshold value for delineation of genomic prokaryotic species.
29458462	4	60	theme	l-1	411:413	arg1	NaCl					415:418	5.0 g l-1 NaCl	405:418	5.0 g l-1 NaCl	405:418	Optimal growth occurred at 37 °C, pH 6.5 with 5.0 g l-1 NaCl.
29458462	12	61	theme	genomic	1295:1301	arg1	DNA					1303:1305	the genomic DNA	1291:1305	the genomic DNA of strain Bb 2-3T	1291:1323	The G+C content of the genomic DNA of strain Bb 2-3T was 37.4 mol%.
29458462	11	62	theme	species	1263:1269	arg1	delineation					1228:1238	delineation	1228:1238	delineation of genomic prokaryotic species	1228:1269	The average nucleotide identity between strain Bb 2-3T and the type strains of closely related species was lower than the 95-96 % threshold value for delineation of genomic prokaryotic species.
29458462	2	63	theme	novel	74:78	arg1	2-3T					234:237	designated strain Bb 2-3T	213:237	designated strain Bb 2-3T	213:237	A novel facultatively anaerobic, Gram-stain-positive, non-motile, non-spore-forming, catalase-negative bacterium of the genus Lactobacillus, designated strain Bb 2-3T, was isolated from bee bread of Apis cerana collected from a hive in Kunming, China.
29458462	2	63	theme	novel	74:78	arg1	bacterium					175:183	A novel facultatively anaerobic, Gram-stain-positive, non-motile, non-spore-forming, catalase-negative bacterium	72:183	A novel facultatively anaerobic, Gram-stain-positive, non-motile, non-spore-forming, catalase-negative bacterium of the genus Lactobacillus	72:210	A novel facultatively anaerobic, Gram-stain-positive, non-motile, non-spore-forming, catalase-negative bacterium of the genus Lactobacillus, designated strain Bb 2-3T, was isolated from bee bread of Apis cerana collected from a hive in Kunming, China.
29458462	2	64	theme	Bb	231:232	arg1	2-3T					234:237	designated strain Bb 2-3T	213:237	designated strain Bb 2-3T	213:237	A novel facultatively anaerobic, Gram-stain-positive, non-motile, non-spore-forming, catalase-negative bacterium of the genus Lactobacillus, designated strain Bb 2-3T, was isolated from bee bread of Apis cerana collected from a hive in Kunming, China.
29458462	2	64	theme	Bb	231:232	arg1	bacterium					175:183	A novel facultatively anaerobic, Gram-stain-positive, non-motile, non-spore-forming, catalase-negative bacterium	72:183	A novel facultatively anaerobic, Gram-stain-positive, non-motile, non-spore-forming, catalase-negative bacterium of the genus Lactobacillus	72:210	A novel facultatively anaerobic, Gram-stain-positive, non-motile, non-spore-forming, catalase-negative bacterium of the genus Lactobacillus, designated strain Bb 2-3T, was isolated from bee bread of Apis cerana collected from a hive in Kunming, China.
29458462	9	65	theme	Lactobacillus	720:732	arg1	28793T					748:753	Lactobacillus bombicola DSM 28793T	720:753	Lactobacillus bombicola DSM 28793T	720:753	Strain Bb 2-3T was closely related to Lactobacillus bombicola DSM 28793T, Lactobacillus apis LMG 26964T and Lactobacillus helsingborgensis DSM 26265T, with 97.8, 97.6 and 97.0 % 16S rRNA gene sequence similarity, respectively.
29458462	10	66	theme	strain	978:983	arg1	2-3T					988:991	strain Bb 2-3T	978:991	strain Bb 2-3T	978:991	A comparison of two housekeeping genes, rpoA and pheS, revealed that strain Bb 2-3T was well separated from the reference strains of species of the genus Lactobacillus.
29458462	13	67	theme	Bb	1417:1418	arg1	2-3T					1420:1423	strain Bb 2-3T	1410:1423	strain Bb 2-3T	1410:1423	On the basis of phenotypic, chemotaxonomic and phylogenetic analyses, strain Bb 2-3T is proposed to represent a novel species of the genus Lactobacillus, for which we propose the name Lactobacillus panisapium sp.
29458462	9	68	theme	DSM	744:746	arg1	28793T					748:753	Lactobacillus bombicola DSM 28793T	720:753	Lactobacillus bombicola DSM 28793T	720:753	Strain Bb 2-3T was closely related to Lactobacillus bombicola DSM 28793T, Lactobacillus apis LMG 26964T and Lactobacillus helsingborgensis DSM 26265T, with 97.8, 97.6 and 97.0 % 16S rRNA gene sequence similarity, respectively.
29458462	15	69	theme	102188T=ACCC	1591:1602	arg1	19955T					1604:1609	=DSM 102188T=ACCC 19955T	1586:1609	=DSM 102188T=ACCC 19955T	1586:1609	The type strain is Bb 2-3T (=DSM 102188T=ACCC 19955T).
29458462	15	69	theme	102188T=ACCC	1591:1602	arg1	2-3T					1580:1583	Bb 2-3T	1577:1583	Bb 2-3T (=DSM 102188T=ACCC 19955T)	1577:1610	The type strain is Bb 2-3T (=DSM 102188T=ACCC 19955T).
29458462	13	70	theme	name	1519:1522	arg1	sp					1549:1550	the name Lactobacillus panisapium sp	1515:1550	the name Lactobacillus panisapium sp	1515:1550	On the basis of phenotypic, chemotaxonomic and phylogenetic analyses, strain Bb 2-3T is proposed to represent a novel species of the genus Lactobacillus, for which we propose the name Lactobacillus panisapium sp.
29458462	9	71	theme	apis	770:773	arg1	26964T					779:784	Lactobacillus apis LMG 26964T	756:784	Lactobacillus apis LMG 26964T	756:784	Strain Bb 2-3T was closely related to Lactobacillus bombicola DSM 28793T, Lactobacillus apis LMG 26964T and Lactobacillus helsingborgensis DSM 26265T, with 97.8, 97.6 and 97.0 % 16S rRNA gene sequence similarity, respectively.
29458462	1	72	theme	Apis	49:52	arg1	bread					65:69	honeybee Apis cerana bee bread	40:69	honeybee Apis cerana bee bread	40:69	nov., from honeybee Apis cerana bee bread.
29458462	10	73	theme	reference	1021:1029	arg1	strains					1031:1037	the reference strains	1017:1037	the reference strains of species of the genus Lactobacillus	1017:1075	A comparison of two housekeeping genes, rpoA and pheS, revealed that strain Bb 2-3T was well separated from the reference strains of species of the genus Lactobacillus.
29458462	1	74	theme	bee	61:63	arg1	bread					65:69	honeybee Apis cerana bee bread	40:69	honeybee Apis cerana bee bread	40:69	nov., from honeybee Apis cerana bee bread.
29458462	9	75	theme	Lactobacillus	790:802	arg1	26265T					825:830	Lactobacillus helsingborgensis DSM 26265T	790:830	Lactobacillus helsingborgensis DSM 26265T	790:830	Strain Bb 2-3T was closely related to Lactobacillus bombicola DSM 28793T, Lactobacillus apis LMG 26964T and Lactobacillus helsingborgensis DSM 26265T, with 97.8, 97.6 and 97.0 % 16S rRNA gene sequence similarity, respectively.
29458462	9	76	dep	Lactobacillus	720:732	arg1	bombicola					734:742	bombicola	734:742	bombicola	734:742	Strain Bb 2-3T was closely related to Lactobacillus bombicola DSM 28793T, Lactobacillus apis LMG 26964T and Lactobacillus helsingborgensis DSM 26265T, with 97.8, 97.6 and 97.0 % 16S rRNA gene sequence similarity, respectively.
29458462	0	77	theme	panisapium	14:23	arg1	sp					25:26	Lactobacillus panisapium sp	0:26	Lactobacillus panisapium sp.	0:27	Lactobacillus panisapium sp.
29458462	11	78	theme	threshold	1208:1216	arg1	value					1218:1222	the 95-96 % threshold value	1196:1222	the 95-96 % threshold value for delineation of genomic prokaryotic species	1196:1269	The average nucleotide identity between strain Bb 2-3T and the type strains of closely related species was lower than the 95-96 % threshold value for delineation of genomic prokaryotic species.
29458462	13	79	theme	strain	1410:1415	arg1	2-3T					1420:1423	strain Bb 2-3T	1410:1423	strain Bb 2-3T	1410:1423	On the basis of phenotypic, chemotaxonomic and phylogenetic analyses, strain Bb 2-3T is proposed to represent a novel species of the genus Lactobacillus, for which we propose the name Lactobacillus panisapium sp.
29458462	11	80	theme	strain	1118:1123	arg1	2-3T					1128:1131	strain Bb 2-3T	1118:1131	strain Bb 2-3T	1118:1131	The average nucleotide identity between strain Bb 2-3T and the type strains of closely related species was lower than the 95-96 % threshold value for delineation of genomic prokaryotic species.
29458462	13	81	theme	panisapium	1538:1547	arg1	sp					1549:1550	the name Lactobacillus panisapium sp	1515:1550	the name Lactobacillus panisapium sp	1515:1550	On the basis of phenotypic, chemotaxonomic and phylogenetic analyses, strain Bb 2-3T is proposed to represent a novel species of the genus Lactobacillus, for which we propose the name Lactobacillus panisapium sp.
29458462	9	82	dep	%	858:858	arg1	97.6					844:847	97.6	844:847	97.6	844:847	Strain Bb 2-3T was closely related to Lactobacillus bombicola DSM 28793T, Lactobacillus apis LMG 26964T and Lactobacillus helsingborgensis DSM 26265T, with 97.8, 97.6 and 97.0 % 16S rRNA gene sequence similarity, respectively.
29458462	9	82	dep	%	858:858	arg1	97.0 					853:857	97.0 	853:857	97.0 	853:857	Strain Bb 2-3T was closely related to Lactobacillus bombicola DSM 28793T, Lactobacillus apis LMG 26964T and Lactobacillus helsingborgensis DSM 26265T, with 97.8, 97.6 and 97.0 % 16S rRNA gene sequence similarity, respectively.
29458462	11	83	theme	type	1141:1144	arg1	strains					1146:1152	the type strains	1137:1152	the type strains of closely related species	1137:1179	The average nucleotide identity between strain Bb 2-3T and the type strains of closely related species was lower than the 95-96 % threshold value for delineation of genomic prokaryotic species.
29458462	2	84	theme	designated	213:222	arg1	2-3T					234:237	designated strain Bb 2-3T	213:237	designated strain Bb 2-3T	213:237	A novel facultatively anaerobic, Gram-stain-positive, non-motile, non-spore-forming, catalase-negative bacterium of the genus Lactobacillus, designated strain Bb 2-3T, was isolated from bee bread of Apis cerana collected from a hive in Kunming, China.
29458462	2	84	theme	designated	213:222	arg1	bacterium					175:183	A novel facultatively anaerobic, Gram-stain-positive, non-motile, non-spore-forming, catalase-negative bacterium	72:183	A novel facultatively anaerobic, Gram-stain-positive, non-motile, non-spore-forming, catalase-negative bacterium of the genus Lactobacillus	72:210	A novel facultatively anaerobic, Gram-stain-positive, non-motile, non-spore-forming, catalase-negative bacterium of the genus Lactobacillus, designated strain Bb 2-3T, was isolated from bee bread of Apis cerana collected from a hive in Kunming, China.
29458462	10	85	theme	genus	1057:1061	arg1	Lactobacillus					1063:1075	the genus Lactobacillus	1053:1075	the genus Lactobacillus	1053:1075	A comparison of two housekeeping genes, rpoA and pheS, revealed that strain Bb 2-3T was well separated from the reference strains of species of the genus Lactobacillus.
29458462	11	86	theme	related	1165:1171	arg1	species					1173:1179	closely related species	1157:1179	closely related species	1157:1179	The average nucleotide identity between strain Bb 2-3T and the type strains of closely related species was lower than the 95-96 % threshold value for delineation of genomic prokaryotic species.
29458462	2	87	theme	Lactobacillus	198:210	arg1	2-3T					234:237	designated strain Bb 2-3T	213:237	designated strain Bb 2-3T	213:237	A novel facultatively anaerobic, Gram-stain-positive, non-motile, non-spore-forming, catalase-negative bacterium of the genus Lactobacillus, designated strain Bb 2-3T, was isolated from bee bread of Apis cerana collected from a hive in Kunming, China.
29458462	2	87	theme	Lactobacillus	198:210	arg1	bacterium					175:183	A novel facultatively anaerobic, Gram-stain-positive, non-motile, non-spore-forming, catalase-negative bacterium	72:183	A novel facultatively anaerobic, Gram-stain-positive, non-motile, non-spore-forming, catalase-negative bacterium of the genus Lactobacillus	72:210	A novel facultatively anaerobic, Gram-stain-positive, non-motile, non-spore-forming, catalase-negative bacterium of the genus Lactobacillus, designated strain Bb 2-3T, was isolated from bee bread of Apis cerana collected from a hive in Kunming, China.
29458462	10	88	theme	housekeeping	929:940	arg1	pheS					958:961	pheS	958:961	pheS	958:961	A comparison of two housekeeping genes, rpoA and pheS, revealed that strain Bb 2-3T was well separated from the reference strains of species of the genus Lactobacillus.
29458462	10	88	theme	housekeeping	929:940	arg1	rpoA					949:952	rpoA	949:952	rpoA	949:952	A comparison of two housekeeping genes, rpoA and pheS, revealed that strain Bb 2-3T was well separated from the reference strains of species of the genus Lactobacillus.
29458462	10	88	theme	housekeeping	929:940	arg1	genes					942:946	two housekeeping genes	925:946	two housekeeping genes	925:946	A comparison of two housekeeping genes, rpoA and pheS, revealed that strain Bb 2-3T was well separated from the reference strains of species of the genus Lactobacillus.
29458462	9	89	theme	16S	860:862	arg1	similarity					883:892	97.8, 97.6 and 97.0 % 16S rRNA gene sequence similarity	838:892	97.8, 97.6 and 97.0 % 16S rRNA gene sequence similarity	838:892	Strain Bb 2-3T was closely related to Lactobacillus bombicola DSM 28793T, Lactobacillus apis LMG 26964T and Lactobacillus helsingborgensis DSM 26265T, with 97.8, 97.6 and 97.0 % 16S rRNA gene sequence similarity, respectively.
30445965	10	0	theme	AidP	1572:1575	arg1	activity					1560:1567	activity	1560:1567	activity of AidP	1560:1575	Furthermore, we demonstrated the effects of covalent and ionic bonding, showing that Zn2+ is important for activity of AidP in vivo.
30445965	6	1	theme	aidP	986:989	arg1	gene					991:994	the aidP gene	982:994	the aidP gene	982:994	Multiple sequence alignment analysis and phylogenetic analysis suggested that the aidP gene encodes a novel AHL lactonase enzyme.
30445965	6	1	theme	aidP	986:989	arg1	enzyme					1026:1031	a novel AHL lactonase enzyme	1004:1031	a novel AHL lactonase enzyme	1004:1031	Multiple sequence alignment analysis and phylogenetic analysis suggested that the aidP gene encodes a novel AHL lactonase enzyme.
30445965	14	2	theme	Chinese	2234:2240	arg1	cabbage					2242:2248	Chinese cabbage	2234:2248	Chinese cabbage	2234:2248	Since the enzyme is most active below human body temperature (below 28 °C), and lose its activity drastically above 32 °C, the results of a pectinolytic inhibition assay using Chinese cabbage indicated the potential of this anti-quorum sensing agent to be safely applied in the field trials.
30445965	11	3	theme	Chinese	1728:1734	arg1	cabbage					1736:1742	Chinese cabbage	1728:1742	Chinese cabbage	1728:1742	The pectinolytic inhibition assay confirmed that this enzyme attenuated the pathogenicity of the plant pathogen Pectobacterium carotovorum in Chinese cabbage.
30445965	12	4	dep	CONCLUSION	1745:1754	arg1	demonstrated					1759:1770	demonstrated	1759:1770	demonstrated that AidP is effective in attenuating the pathogenicity of P. carotovorum, a plant pathogen that causes soft-rot disease	1759:1891	CONCLUSION We demonstrated that AidP is effective in attenuating the pathogenicity of P. carotovorum, a plant pathogen that causes soft-rot disease.
30445965	12	5	theme	carotovorum	1834:1844	arg1	pathogenicity					1814:1826	the pathogenicity	1810:1826	the pathogenicity of P. carotovorum, a plant pathogen that causes soft-rot disease	1810:1891	CONCLUSION We demonstrated that AidP is effective in attenuating the pathogenicity of P. carotovorum, a plant pathogen that causes soft-rot disease.
30445965	4	6	theme	substituted	701:711	arg1	lactones					724:731	unsubstituted, and 3-oxo substituted homoserine lactones	676:731	unsubstituted, and 3-oxo substituted homoserine lactones	676:731	RESULTS Rapid resolution liquid chromatography analysis indicated that purified AidP possesses high AHL-degrading activity on unsubstituted, and 3-oxo substituted homoserine lactones.
30445965	14	7	theme	assay	2222:2226	arg1	results					2185:2191	the results	2181:2191	the results of a pectinolytic inhibition assay using Chinese cabbage	2181:2248	Since the enzyme is most active below human body temperature (below 28 °C), and lose its activity drastically above 32 °C, the results of a pectinolytic inhibition assay using Chinese cabbage indicated the potential of this anti-quorum sensing agent to be safely applied in the field trials.
30445965	11	8	theme	carotovorum	1713:1723	arg1	pathogenicity					1662:1674	the pathogenicity	1658:1674	the pathogenicity of the plant pathogen Pectobacterium carotovorum in Chinese cabbage	1658:1742	The pectinolytic inhibition assay confirmed that this enzyme attenuated the pathogenicity of the plant pathogen Pectobacterium carotovorum in Chinese cabbage.
30445965	11	9	theme	inhibition	1603:1612	arg1	assay					1614:1618	The pectinolytic inhibition assay	1586:1618	The pectinolytic inhibition assay	1586:1618	The pectinolytic inhibition assay confirmed that this enzyme attenuated the pathogenicity of the plant pathogen Pectobacterium carotovorum in Chinese cabbage.
30445965	8	10	theme	aidP	1304:1307	arg1	genes					1309:1313	several aidP genes	1296:1313	several aidP genes of Planococcus sp	1296:1331	Branch-site analysis of several aidP genes of Planococcus sp.
30445965	14	11	theme	pectinolytic	2198:2209	arg1	assay					2222:2226	a pectinolytic inhibition assay	2196:2226	a pectinolytic inhibition assay using Chinese cabbage	2196:2248	Since the enzyme is most active below human body temperature (below 28 °C), and lose its activity drastically above 32 °C, the results of a pectinolytic inhibition assay using Chinese cabbage indicated the potential of this anti-quorum sensing agent to be safely applied in the field trials.
30445965	1	12	theme	Gram-negative	192:204	arg1	Proteobacteria					206:219	some Gram-negative Proteobacteria	187:219	some Gram-negative Proteobacteria for bacterial cell-to-cell communication or quorum sensing	187:278	BACKGROUND N-acylhomoserine lactones (AHLs) are well-studied signalling molecules produced by some Gram-negative Proteobacteria for bacterial cell-to-cell communication or quorum sensing.
30445965	4	13	theme	unsubstituted	676:688	arg1	lactones					724:731	unsubstituted, and 3-oxo substituted homoserine lactones	676:731	unsubstituted, and 3-oxo substituted homoserine lactones	676:731	RESULTS Rapid resolution liquid chromatography analysis indicated that purified AidP possesses high AHL-degrading activity on unsubstituted, and 3-oxo substituted homoserine lactones.
30445965	2	14	theme	AHLs	332:335	arg1	degradation					317:327	the degradation	313:327	the degradation of AHLs by an Antarctic bacterium, Planococcus versutus L10.15T,	313:392	We have previously demonstrated the degradation of AHLs by an Antarctic bacterium, Planococcus versutus L10.15T, at low temperature through the production of an AHL lactonase.
30445965	8	15	theme	sp	1330:1331	arg1	genes					1309:1313	several aidP genes	1296:1313	several aidP genes of Planococcus sp	1296:1331	Branch-site analysis of several aidP genes of Planococcus sp.
30445965	0	16	theme	novel	22:26	arg1	lactonase					45:53	a novel N-acylhomoserine lactonase	20:53	a novel N-acylhomoserine lactonase	20:53	Characterization of a novel N-acylhomoserine lactonase, AidP, from Antarctic Planococcus sp.
30445965	0	16	theme	novel	22:26	arg1	AidP					56:59	AidP	56:59	AidP	56:59	Characterization of a novel N-acylhomoserine lactonase, AidP, from Antarctic Planococcus sp.
30445965	7	17	theme	homologous	1086:1095	arg1	genes					1097:1101	the homologous genes	1082:1101	the homologous genes	1082:1101	The amino acid composition analysis of aidP and the homologous genes suggested that it might be a cold-adapted enzyme, however, the optimum temperature is 28 °C, even though the thermal stability is low (reduced drastically above 32 °C).
30445965	5	18	theme	ring	890:893	arg1	bond					859:862	the ester bond	849:862	the ester bond of the homoserine lactone ring of AHLs	849:901	Liquid chromatography-mass spectrometry analysis confirmed that AidP functions as an AHL lactonase that hydrolyzes the ester bond of the homoserine lactone ring of AHLs.
30445965	8	19	theme	Branch-site	1272:1282	arg1	analysis					1284:1291	Branch-site analysis	1272:1291	Branch-site analysis of several aidP genes of Planococcus sp.	1272:1332	Branch-site analysis of several aidP genes of Planococcus sp.
30445965	6	20	theme	lactonase	1016:1024	arg1	gene					991:994	the aidP gene	982:994	the aidP gene	982:994	Multiple sequence alignment analysis and phylogenetic analysis suggested that the aidP gene encodes a novel AHL lactonase enzyme.
30445965	6	20	theme	lactonase	1016:1024	arg1	enzyme					1026:1031	a novel AHL lactonase enzyme	1004:1031	a novel AHL lactonase enzyme	1004:1031	Multiple sequence alignment analysis and phylogenetic analysis suggested that the aidP gene encodes a novel AHL lactonase enzyme.
30445965	5	21	theme	Liquid	734:739	arg1	analysis					774:781	Liquid chromatography-mass spectrometry analysis	734:781	Liquid chromatography-mass spectrometry analysis	734:781	Liquid chromatography-mass spectrometry analysis confirmed that AidP functions as an AHL lactonase that hydrolyzes the ester bond of the homoserine lactone ring of AHLs.
30445965	6	22	theme	novel	1006:1010	arg1	gene					991:994	the aidP gene	982:994	the aidP gene	982:994	Multiple sequence alignment analysis and phylogenetic analysis suggested that the aidP gene encodes a novel AHL lactonase enzyme.
30445965	6	22	theme	novel	1006:1010	arg1	enzyme					1026:1031	a novel AHL lactonase enzyme	1004:1031	a novel AHL lactonase enzyme	1004:1031	Multiple sequence alignment analysis and phylogenetic analysis suggested that the aidP gene encodes a novel AHL lactonase enzyme.
30445965	5	23	theme	spectrometry	761:772	arg1	analysis					774:781	Liquid chromatography-mass spectrometry analysis	734:781	Liquid chromatography-mass spectrometry analysis	734:781	Liquid chromatography-mass spectrometry analysis confirmed that AidP functions as an AHL lactonase that hydrolyzes the ester bond of the homoserine lactone ring of AHLs.
30445965	14	24	theme	sensing	2294:2300	arg1	agent					2302:2306	this anti-quorum sensing agent	2277:2306	this anti-quorum sensing agent	2277:2306	Since the enzyme is most active below human body temperature (below 28 °C), and lose its activity drastically above 32 °C, the results of a pectinolytic inhibition assay using Chinese cabbage indicated the potential of this anti-quorum sensing agent to be safely applied in the field trials.
30445965	0	25	from	sp	89:90	arg1	lactonase					45:53	a novel N-acylhomoserine lactonase	20:53	a novel N-acylhomoserine lactonase	20:53	Characterization of a novel N-acylhomoserine lactonase, AidP, from Antarctic Planococcus sp.
30445965	0	25	from	sp	89:90	arg1	Characterization					0:15	Characterization	0:15	Characterization of a novel N-acylhomoserine lactonase, AidP, from Antarctic Planococcus sp.	0:91	Characterization of a novel N-acylhomoserine lactonase, AidP, from Antarctic Planococcus sp.
30445965	0	25	from	sp	89:90	arg1	AidP					56:59	AidP	56:59	AidP	56:59	Characterization of a novel N-acylhomoserine lactonase, AidP, from Antarctic Planococcus sp.
30445965	13	26	theme	signalling	1993:2002	arg1	AHLs					2015:2018	AHLs	2015:2018	AHLs	2015:2018	This anti-quorum sensing agent is an enzyme with low thermal stability that degrades the bacterial signalling molecules (AHLs) that are produced by many pathogens.
30445965	13	26	theme	signalling	1993:2002	arg1	molecules					2004:2012	the bacterial signalling molecules	1979:2012	the bacterial signalling molecules (AHLs) that are produced by many pathogens	1979:2055	This anti-quorum sensing agent is an enzyme with low thermal stability that degrades the bacterial signalling molecules (AHLs) that are produced by many pathogens.
30445965	7	27	theme	composition	1049:1059	arg1	analysis					1061:1068	The amino acid composition analysis	1034:1068	The amino acid composition analysis of aidP and the homologous genes	1034:1101	The amino acid composition analysis of aidP and the homologous genes suggested that it might be a cold-adapted enzyme, however, the optimum temperature is 28 °C, even though the thermal stability is low (reduced drastically above 32 °C).
30445965	1	28	theme	well-studied	141:152	arg1	lactones					121:128	BACKGROUND N-acylhomoserine lactones	93:128	BACKGROUND N-acylhomoserine lactones (AHLs)	93:135	BACKGROUND N-acylhomoserine lactones (AHLs) are well-studied signalling molecules produced by some Gram-negative Proteobacteria for bacterial cell-to-cell communication or quorum sensing.
30445965	1	28	theme	well-studied	141:152	arg1	molecules					165:173	well-studied signalling molecules	141:173	well-studied signalling molecules produced by some Gram-negative Proteobacteria for bacterial cell-to-cell communication or quorum sensing	141:278	BACKGROUND N-acylhomoserine lactones (AHLs) are well-studied signalling molecules produced by some Gram-negative Proteobacteria for bacterial cell-to-cell communication or quorum sensing.
30445965	10	29	theme	covalent	1497:1504	arg1	bonding					1516:1522	covalent and ionic bonding	1497:1522	covalent and ionic bonding	1497:1522	Furthermore, we demonstrated the effects of covalent and ionic bonding, showing that Zn2+ is important for activity of AidP in vivo.
30445965	13	30	theme	thermal	1947:1953	arg1	stability					1955:1963	low thermal stability	1943:1963	low thermal stability	1943:1963	This anti-quorum sensing agent is an enzyme with low thermal stability that degrades the bacterial signalling molecules (AHLs) that are produced by many pathogens.
30445965	0	31	theme	Antarctic	67:75	arg1	sp					89:90	Antarctic Planococcus sp	67:90	Antarctic Planococcus sp	67:90	Characterization of a novel N-acylhomoserine lactonase, AidP, from Antarctic Planococcus sp.
30445965	7	32	theme	amino	1038:1042	arg1	composition					1049:1059	The amino acid composition	1034:1059	The amino acid composition analysis of aidP and the homologous genes	1034:1101	The amino acid composition analysis of aidP and the homologous genes suggested that it might be a cold-adapted enzyme, however, the optimum temperature is 28 °C, even though the thermal stability is low (reduced drastically above 32 °C).
30445965	0	33	from	Characterization	0:15	arg1	sp					89:90	Antarctic Planococcus sp	67:90	Antarctic Planococcus sp	67:90	Characterization of a novel N-acylhomoserine lactonase, AidP, from Antarctic Planococcus sp.
30445965	9	34	theme	phylogenetic	1348:1359	arg1	trees					1361:1365	the phylogenetic trees	1344:1365	the phylogenetic trees	1344:1365	branch on the phylogenetic trees also showed evidence of episodic positive selection of the gene in cold environments.
30445965	14	35	theme	anti-quorum	2282:2292	arg1	agent					2302:2306	this anti-quorum sensing agent	2277:2306	this anti-quorum sensing agent	2277:2306	Since the enzyme is most active below human body temperature (below 28 °C), and lose its activity drastically above 32 °C, the results of a pectinolytic inhibition assay using Chinese cabbage indicated the potential of this anti-quorum sensing agent to be safely applied in the field trials.
30445965	5	36	theme	AHL	819:821	arg1	lactonase					823:831	an AHL lactonase	816:831	an AHL lactonase that hydrolyzes the ester bond of the homoserine lactone ring of AHLs	816:901	Liquid chromatography-mass spectrometry analysis confirmed that AidP functions as an AHL lactonase that hydrolyzes the ester bond of the homoserine lactone ring of AHLs.
30445965	5	36	theme	AHL	819:821	arg1	AidP					798:801	AidP	798:801	AidP	798:801	Liquid chromatography-mass spectrometry analysis confirmed that AidP functions as an AHL lactonase that hydrolyzes the ester bond of the homoserine lactone ring of AHLs.
30445965	0	37	theme	lactonase	45:53	arg1	Characterization					0:15	Characterization	0:15	Characterization of a novel N-acylhomoserine lactonase, AidP, from Antarctic Planococcus sp.	0:91	Characterization of a novel N-acylhomoserine lactonase, AidP, from Antarctic Planococcus sp.
30445965	10	38	theme	ionic	1510:1514	arg1	bonding					1516:1522	covalent and ionic bonding	1497:1522	covalent and ionic bonding	1497:1522	Furthermore, we demonstrated the effects of covalent and ionic bonding, showing that Zn2+ is important for activity of AidP in vivo.
30445965	5	39	theme	lactone	882:888	arg1	ring					890:893	the homoserine lactone ring	867:893	the homoserine lactone ring of AHLs	867:901	Liquid chromatography-mass spectrometry analysis confirmed that AidP functions as an AHL lactonase that hydrolyzes the ester bond of the homoserine lactone ring of AHLs.
30445965	4	40	theme	resolution	564:573	arg1	chromatography					582:595	Rapid resolution liquid chromatography	558:595	Rapid resolution liquid chromatography analysis	558:604	RESULTS Rapid resolution liquid chromatography analysis indicated that purified AidP possesses high AHL-degrading activity on unsubstituted, and 3-oxo substituted homoserine lactones.
30445965	13	41	theme	anti-quorum	1899:1909	arg1	agent					1919:1923	This anti-quorum sensing agent	1894:1923	This anti-quorum sensing agent	1894:1923	This anti-quorum sensing agent is an enzyme with low thermal stability that degrades the bacterial signalling molecules (AHLs) that are produced by many pathogens.
30445965	13	41	theme	anti-quorum	1899:1909	arg1	enzyme					1931:1936	an enzyme	1928:1936	an enzyme with low thermal stability that degrades the bacterial signalling molecules (AHLs) that are produced by many pathogens	1928:2055	This anti-quorum sensing agent is an enzyme with low thermal stability that degrades the bacterial signalling molecules (AHLs) that are produced by many pathogens.
30445965	2	42	theme	lactonase	446:454	arg1	production					425:434	the production	421:434	the production of an AHL lactonase	421:454	We have previously demonstrated the degradation of AHLs by an Antarctic bacterium, Planococcus versutus L10.15T, at low temperature through the production of an AHL lactonase.
30445965	9	43	theme	episodic	1391:1398	arg1	selection					1409:1417	episodic positive selection	1391:1417	episodic positive selection of the gene in cold environments	1391:1450	branch on the phylogenetic trees also showed evidence of episodic positive selection of the gene in cold environments.
30445965	6	44	theme	phylogenetic	945:956	arg1	analysis					958:965	phylogenetic analysis	945:965	phylogenetic analysis	945:965	Multiple sequence alignment analysis and phylogenetic analysis suggested that the aidP gene encodes a novel AHL lactonase enzyme.
30445965	1	45	theme	N-acylhomoserine	104:119	arg1	AHLs					131:134	AHLs	131:134	AHLs	131:134	BACKGROUND N-acylhomoserine lactones (AHLs) are well-studied signalling molecules produced by some Gram-negative Proteobacteria for bacterial cell-to-cell communication or quorum sensing.
30445965	1	45	theme	N-acylhomoserine	104:119	arg1	lactones					121:128	BACKGROUND N-acylhomoserine lactones	93:128	BACKGROUND N-acylhomoserine lactones (AHLs)	93:135	BACKGROUND N-acylhomoserine lactones (AHLs) are well-studied signalling molecules produced by some Gram-negative Proteobacteria for bacterial cell-to-cell communication or quorum sensing.
30445965	1	45	theme	N-acylhomoserine	104:119	arg1	molecules					165:173	well-studied signalling molecules	141:173	well-studied signalling molecules produced by some Gram-negative Proteobacteria for bacterial cell-to-cell communication or quorum sensing	141:278	BACKGROUND N-acylhomoserine lactones (AHLs) are well-studied signalling molecules produced by some Gram-negative Proteobacteria for bacterial cell-to-cell communication or quorum sensing.
30445965	4	46	theme	chromatography	582:595	arg1	analysis					597:604	Rapid resolution liquid chromatography analysis	558:604	Rapid resolution liquid chromatography analysis	558:604	RESULTS Rapid resolution liquid chromatography analysis indicated that purified AidP possesses high AHL-degrading activity on unsubstituted, and 3-oxo substituted homoserine lactones.
30445965	0	47	theme	N-acylhomoserine	28:43	arg1	lactonase					45:53	a novel N-acylhomoserine lactonase	20:53	a novel N-acylhomoserine lactonase	20:53	Characterization of a novel N-acylhomoserine lactonase, AidP, from Antarctic Planococcus sp.
30445965	0	47	theme	N-acylhomoserine	28:43	arg1	AidP					56:59	AidP	56:59	AidP	56:59	Characterization of a novel N-acylhomoserine lactonase, AidP, from Antarctic Planococcus sp.
30445965	9	48	theme	selection	1409:1417	arg1	evidence					1379:1386	evidence	1379:1386	evidence of episodic positive selection of the gene in cold environments	1379:1450	branch on the phylogenetic trees also showed evidence of episodic positive selection of the gene in cold environments.
30445965	12	49	theme	soft-rot	1876:1883	arg1	disease					1885:1891	soft-rot disease	1876:1891	soft-rot disease	1876:1891	CONCLUSION We demonstrated that AidP is effective in attenuating the pathogenicity of P. carotovorum, a plant pathogen that causes soft-rot disease.
30445965	14	50	theme	body	2102:2105	arg1	temperature					2107:2117	human body temperature	2096:2117	human body temperature (below 28 °C)	2096:2131	Since the enzyme is most active below human body temperature (below 28 °C), and lose its activity drastically above 32 °C, the results of a pectinolytic inhibition assay using Chinese cabbage indicated the potential of this anti-quorum sensing agent to be safely applied in the field trials.
30445965	6	51	theme	sequence	913:920	arg1	analysis					932:939	Multiple sequence alignment analysis	904:939	Multiple sequence alignment analysis	904:939	Multiple sequence alignment analysis and phylogenetic analysis suggested that the aidP gene encodes a novel AHL lactonase enzyme.
30445965	3	52	theme	AHL	486:488	arg1	gene					500:503	the AHL lactonase gene	482:503	the AHL lactonase gene	482:503	In this study, we cloned the AHL lactonase gene and characterized the purified novel enzyme.
30445965	12	53	theme	plant	1849:1853	arg1	pathogen					1855:1862	a plant pathogen	1847:1862	a plant pathogen that causes soft-rot disease	1847:1891	CONCLUSION We demonstrated that AidP is effective in attenuating the pathogenicity of P. carotovorum, a plant pathogen that causes soft-rot disease.
30445965	12	53	theme	plant	1849:1853	arg1	carotovorum					1834:1844	P. carotovorum	1831:1844	P. carotovorum	1831:1844	CONCLUSION We demonstrated that AidP is effective in attenuating the pathogenicity of P. carotovorum, a plant pathogen that causes soft-rot disease.
30445965	3	54	theme	purified	527:534	arg1	enzyme					542:547	the purified novel enzyme	523:547	the purified novel enzyme	523:547	In this study, we cloned the AHL lactonase gene and characterized the purified novel enzyme.
30445965	1	55	theme	quorum	265:270	arg1	sensing					272:278	quorum sensing	265:278	quorum sensing	265:278	BACKGROUND N-acylhomoserine lactones (AHLs) are well-studied signalling molecules produced by some Gram-negative Proteobacteria for bacterial cell-to-cell communication or quorum sensing.
30445965	4	56	theme	AHL-degrading	650:662	arg1	activity					664:671	high AHL-degrading activity	645:671	high AHL-degrading activity on unsubstituted, and 3-oxo substituted homoserine lactones	645:731	RESULTS Rapid resolution liquid chromatography analysis indicated that purified AidP possesses high AHL-degrading activity on unsubstituted, and 3-oxo substituted homoserine lactones.
30445965	11	57	theme	Pectobacterium	1698:1711	arg1	carotovorum					1713:1723	the plant pathogen Pectobacterium carotovorum	1679:1723	the plant pathogen Pectobacterium carotovorum	1679:1723	The pectinolytic inhibition assay confirmed that this enzyme attenuated the pathogenicity of the plant pathogen Pectobacterium carotovorum in Chinese cabbage.
30445965	11	58	theme	plant	1683:1687	arg1	carotovorum					1713:1723	the plant pathogen Pectobacterium carotovorum	1679:1723	the plant pathogen Pectobacterium carotovorum	1679:1723	The pectinolytic inhibition assay confirmed that this enzyme attenuated the pathogenicity of the plant pathogen Pectobacterium carotovorum in Chinese cabbage.
30445965	7	59	theme	optimum	1166:1172	arg1	temperature					1174:1184	the optimum temperature	1162:1184	the optimum temperature	1162:1184	The amino acid composition analysis of aidP and the homologous genes suggested that it might be a cold-adapted enzyme, however, the optimum temperature is 28 °C, even though the thermal stability is low (reduced drastically above 32 °C).
30445965	7	59	theme	optimum	1166:1172	arg1	28 °C					1189:1193	28 °C	1189:1193	28 °C	1189:1193	The amino acid composition analysis of aidP and the homologous genes suggested that it might be a cold-adapted enzyme, however, the optimum temperature is 28 °C, even though the thermal stability is low (reduced drastically above 32 °C).
30445965	4	60	theme	purified	621:628	arg1	AidP					630:633	purified AidP	621:633	purified AidP	621:633	RESULTS Rapid resolution liquid chromatography analysis indicated that purified AidP possesses high AHL-degrading activity on unsubstituted, and 3-oxo substituted homoserine lactones.
30445965	14	61	theme	field	2336:2340	arg1	trials					2342:2347	the field trials	2332:2347	the field trials	2332:2347	Since the enzyme is most active below human body temperature (below 28 °C), and lose its activity drastically above 32 °C, the results of a pectinolytic inhibition assay using Chinese cabbage indicated the potential of this anti-quorum sensing agent to be safely applied in the field trials.
30445965	4	62	theme	3-oxo	695:699	arg1	lactones					724:731	unsubstituted, and 3-oxo substituted homoserine lactones	676:731	unsubstituted, and 3-oxo substituted homoserine lactones	676:731	RESULTS Rapid resolution liquid chromatography analysis indicated that purified AidP possesses high AHL-degrading activity on unsubstituted, and 3-oxo substituted homoserine lactones.
30445965	14	63	theme	inhibition	2211:2220	arg1	assay					2222:2226	a pectinolytic inhibition assay	2196:2226	a pectinolytic inhibition assay using Chinese cabbage	2196:2248	Since the enzyme is most active below human body temperature (below 28 °C), and lose its activity drastically above 32 °C, the results of a pectinolytic inhibition assay using Chinese cabbage indicated the potential of this anti-quorum sensing agent to be safely applied in the field trials.
30445965	2	64	theme	Antarctic	343:351	arg1	bacterium					353:361	an Antarctic bacterium	340:361	an Antarctic bacterium	340:361	We have previously demonstrated the degradation of AHLs by an Antarctic bacterium, Planococcus versutus L10.15T, at low temperature through the production of an AHL lactonase.
30445965	2	64	theme	Antarctic	343:351	arg1	L10.15T					385:391	Planococcus versutus L10.15T	364:391	Planococcus versutus L10.15T	364:391	We have previously demonstrated the degradation of AHLs by an Antarctic bacterium, Planococcus versutus L10.15T, at low temperature through the production of an AHL lactonase.
30445965	11	65	from	pathogenicity	1662:1674	arg1	cabbage					1736:1742	Chinese cabbage	1728:1742	Chinese cabbage	1728:1742	The pectinolytic inhibition assay confirmed that this enzyme attenuated the pathogenicity of the plant pathogen Pectobacterium carotovorum in Chinese cabbage.
30445965	4	66	theme	homoserine	713:722	arg1	lactones					724:731	unsubstituted, and 3-oxo substituted homoserine lactones	676:731	unsubstituted, and 3-oxo substituted homoserine lactones	676:731	RESULTS Rapid resolution liquid chromatography analysis indicated that purified AidP possesses high AHL-degrading activity on unsubstituted, and 3-oxo substituted homoserine lactones.
30445965	7	67	theme	cold-adapted	1132:1143	arg1	it					1118:1119	it	1118:1119	it	1118:1119	The amino acid composition analysis of aidP and the homologous genes suggested that it might be a cold-adapted enzyme, however, the optimum temperature is 28 °C, even though the thermal stability is low (reduced drastically above 32 °C).
30445965	7	67	theme	cold-adapted	1132:1143	arg1	enzyme					1145:1150	a cold-adapted enzyme	1130:1150	a cold-adapted enzyme	1130:1150	The amino acid composition analysis of aidP and the homologous genes suggested that it might be a cold-adapted enzyme, however, the optimum temperature is 28 °C, even though the thermal stability is low (reduced drastically above 32 °C).
30445965	11	68	theme	pectinolytic	1590:1601	arg1	assay					1614:1618	The pectinolytic inhibition assay	1586:1618	The pectinolytic inhibition assay	1586:1618	The pectinolytic inhibition assay confirmed that this enzyme attenuated the pathogenicity of the plant pathogen Pectobacterium carotovorum in Chinese cabbage.
30445965	5	69	theme	AHLs	898:901	arg1	ring					890:893	the homoserine lactone ring	867:893	the homoserine lactone ring of AHLs	867:901	Liquid chromatography-mass spectrometry analysis confirmed that AidP functions as an AHL lactonase that hydrolyzes the ester bond of the homoserine lactone ring of AHLs.
30445965	2	70	theme	low	397:399	arg1	temperature					401:411	low temperature	397:411	low temperature	397:411	We have previously demonstrated the degradation of AHLs by an Antarctic bacterium, Planococcus versutus L10.15T, at low temperature through the production of an AHL lactonase.
30445965	8	71	theme	genes	1309:1313	arg1	analysis					1284:1291	Branch-site analysis	1272:1291	Branch-site analysis of several aidP genes of Planococcus sp.	1272:1332	Branch-site analysis of several aidP genes of Planococcus sp.
30445965	8	72	theme	several	1296:1302	arg1	genes					1309:1313	several aidP genes	1296:1313	several aidP genes of Planococcus sp	1296:1331	Branch-site analysis of several aidP genes of Planococcus sp.
30445965	1	73	theme	bacterial	225:233	arg1	communication					248:260	bacterial cell-to-cell communication	225:260	bacterial cell-to-cell communication	225:260	BACKGROUND N-acylhomoserine lactones (AHLs) are well-studied signalling molecules produced by some Gram-negative Proteobacteria for bacterial cell-to-cell communication or quorum sensing.
30445965	8	74	theme	Planococcus	1318:1328	arg1	sp					1330:1331	Planococcus sp	1318:1331	Planococcus sp	1318:1331	Branch-site analysis of several aidP genes of Planococcus sp.
30445965	7	75	theme	genes	1097:1101	arg1	analysis					1061:1068	The amino acid composition analysis	1034:1068	The amino acid composition analysis of aidP and the homologous genes	1034:1101	The amino acid composition analysis of aidP and the homologous genes suggested that it might be a cold-adapted enzyme, however, the optimum temperature is 28 °C, even though the thermal stability is low (reduced drastically above 32 °C).
30445965	9	76	from	branch	1334:1339	arg1	trees					1361:1365	the phylogenetic trees	1344:1365	the phylogenetic trees	1344:1365	branch on the phylogenetic trees also showed evidence of episodic positive selection of the gene in cold environments.
30445965	6	77	theme	AHL	1012:1014	arg1	gene					991:994	the aidP gene	982:994	the aidP gene	982:994	Multiple sequence alignment analysis and phylogenetic analysis suggested that the aidP gene encodes a novel AHL lactonase enzyme.
30445965	6	77	theme	AHL	1012:1014	arg1	enzyme					1026:1031	a novel AHL lactonase enzyme	1004:1031	a novel AHL lactonase enzyme	1004:1031	Multiple sequence alignment analysis and phylogenetic analysis suggested that the aidP gene encodes a novel AHL lactonase enzyme.
30445965	13	78	with	enzyme	1931:1936	arg1	stability					1955:1963	low thermal stability	1943:1963	low thermal stability	1943:1963	This anti-quorum sensing agent is an enzyme with low thermal stability that degrades the bacterial signalling molecules (AHLs) that are produced by many pathogens.
30445965	5	79	theme	chromatography-mass	741:759	arg1	analysis					774:781	Liquid chromatography-mass spectrometry analysis	734:781	Liquid chromatography-mass spectrometry analysis	734:781	Liquid chromatography-mass spectrometry analysis confirmed that AidP functions as an AHL lactonase that hydrolyzes the ester bond of the homoserine lactone ring of AHLs.
30445965	13	80	theme	bacterial	1983:1991	arg1	AHLs					2015:2018	AHLs	2015:2018	AHLs	2015:2018	This anti-quorum sensing agent is an enzyme with low thermal stability that degrades the bacterial signalling molecules (AHLs) that are produced by many pathogens.
30445965	13	80	theme	bacterial	1983:1991	arg1	molecules					2004:2012	the bacterial signalling molecules	1979:2012	the bacterial signalling molecules (AHLs) that are produced by many pathogens	1979:2055	This anti-quorum sensing agent is an enzyme with low thermal stability that degrades the bacterial signalling molecules (AHLs) that are produced by many pathogens.
30445965	4	81	from	activity	664:671	arg1	lactones					724:731	unsubstituted, and 3-oxo substituted homoserine lactones	676:731	unsubstituted, and 3-oxo substituted homoserine lactones	676:731	RESULTS Rapid resolution liquid chromatography analysis indicated that purified AidP possesses high AHL-degrading activity on unsubstituted, and 3-oxo substituted homoserine lactones.
30445965	4	82	contain	possesses	635:643	arg2	activity					664:671	high AHL-degrading activity	645:671	high AHL-degrading activity on unsubstituted, and 3-oxo substituted homoserine lactones	645:731	RESULTS Rapid resolution liquid chromatography analysis indicated that purified AidP possesses high AHL-degrading activity on unsubstituted, and 3-oxo substituted homoserine lactones.
30445965	4	82	contain	possesses	635:643	arg1	AidP					630:633	purified AidP	621:633	purified AidP	621:633	RESULTS Rapid resolution liquid chromatography analysis indicated that purified AidP possesses high AHL-degrading activity on unsubstituted, and 3-oxo substituted homoserine lactones.
30445965	7	83	theme	aidP	1073:1076	arg1	analysis					1061:1068	The amino acid composition analysis	1034:1068	The amino acid composition analysis of aidP and the homologous genes	1034:1101	The amino acid composition analysis of aidP and the homologous genes suggested that it might be a cold-adapted enzyme, however, the optimum temperature is 28 °C, even though the thermal stability is low (reduced drastically above 32 °C).
30445965	13	84	theme	low	1943:1945	arg1	stability					1955:1963	low thermal stability	1943:1963	low thermal stability	1943:1963	This anti-quorum sensing agent is an enzyme with low thermal stability that degrades the bacterial signalling molecules (AHLs) that are produced by many pathogens.
30445965	0	85	theme	Planococcus	77:87	arg1	sp					89:90	Antarctic Planococcus sp	67:90	Antarctic Planococcus sp	67:90	Characterization of a novel N-acylhomoserine lactonase, AidP, from Antarctic Planococcus sp.
30445965	4	86	dep	RESULTS	550:556	arg1	indicated					606:614	indicated	606:614	indicated that purified AidP possesses high AHL-degrading activity on unsubstituted, and 3-oxo substituted homoserine lactones	606:731	RESULTS Rapid resolution liquid chromatography analysis indicated that purified AidP possesses high AHL-degrading activity on unsubstituted, and 3-oxo substituted homoserine lactones.
30445965	1	87	theme	signalling	154:163	arg1	lactones					121:128	BACKGROUND N-acylhomoserine lactones	93:128	BACKGROUND N-acylhomoserine lactones (AHLs)	93:135	BACKGROUND N-acylhomoserine lactones (AHLs) are well-studied signalling molecules produced by some Gram-negative Proteobacteria for bacterial cell-to-cell communication or quorum sensing.
30445965	1	87	theme	signalling	154:163	arg1	molecules					165:173	well-studied signalling molecules	141:173	well-studied signalling molecules produced by some Gram-negative Proteobacteria for bacterial cell-to-cell communication or quorum sensing	141:278	BACKGROUND N-acylhomoserine lactones (AHLs) are well-studied signalling molecules produced by some Gram-negative Proteobacteria for bacterial cell-to-cell communication or quorum sensing.
30445965	9	88	from	selection	1409:1417	arg1	environments					1439:1450	cold environments	1434:1450	cold environments	1434:1450	branch on the phylogenetic trees also showed evidence of episodic positive selection of the gene in cold environments.
30445965	7	89	theme	acid	1044:1047	arg1	composition					1049:1059	The amino acid composition	1034:1059	The amino acid composition analysis of aidP and the homologous genes	1034:1101	The amino acid composition analysis of aidP and the homologous genes suggested that it might be a cold-adapted enzyme, however, the optimum temperature is 28 °C, even though the thermal stability is low (reduced drastically above 32 °C).
30445965	10	90	theme	bonding	1516:1522	arg1	effects					1486:1492	the effects	1482:1492	the effects of covalent and ionic bonding	1482:1522	Furthermore, we demonstrated the effects of covalent and ionic bonding, showing that Zn2+ is important for activity of AidP in vivo.
30445965	5	91	theme	homoserine	871:880	arg1	ring					890:893	the homoserine lactone ring	867:893	the homoserine lactone ring of AHLs	867:901	Liquid chromatography-mass spectrometry analysis confirmed that AidP functions as an AHL lactonase that hydrolyzes the ester bond of the homoserine lactone ring of AHLs.
30445965	9	92	from	evidence	1379:1386	arg1	environments					1439:1450	cold environments	1434:1450	cold environments	1434:1450	branch on the phylogenetic trees also showed evidence of episodic positive selection of the gene in cold environments.
30445965	4	93	theme	liquid	575:580	arg1	chromatography					582:595	Rapid resolution liquid chromatography	558:595	Rapid resolution liquid chromatography analysis	558:604	RESULTS Rapid resolution liquid chromatography analysis indicated that purified AidP possesses high AHL-degrading activity on unsubstituted, and 3-oxo substituted homoserine lactones.
30445965	14	94	theme	agent	2302:2306	arg1	potential					2264:2272	the potential	2260:2272	the potential of this anti-quorum sensing agent to be safely applied in the field trials	2260:2347	Since the enzyme is most active below human body temperature (below 28 °C), and lose its activity drastically above 32 °C, the results of a pectinolytic inhibition assay using Chinese cabbage indicated the potential of this anti-quorum sensing agent to be safely applied in the field trials.
30445965	7	95	theme	thermal	1212:1218	arg1	stability					1220:1228	the thermal stability	1208:1228	the thermal stability	1208:1228	The amino acid composition analysis of aidP and the homologous genes suggested that it might be a cold-adapted enzyme, however, the optimum temperature is 28 °C, even though the thermal stability is low (reduced drastically above 32 °C).
30445965	7	95	theme	thermal	1212:1218	arg1	low					1233:1235	low	1233:1235	low	1233:1235	The amino acid composition analysis of aidP and the homologous genes suggested that it might be a cold-adapted enzyme, however, the optimum temperature is 28 °C, even though the thermal stability is low (reduced drastically above 32 °C).
30445965	1	96	theme	BACKGROUND	93:102	arg1	AHLs					131:134	AHLs	131:134	AHLs	131:134	BACKGROUND N-acylhomoserine lactones (AHLs) are well-studied signalling molecules produced by some Gram-negative Proteobacteria for bacterial cell-to-cell communication or quorum sensing.
30445965	1	96	theme	BACKGROUND	93:102	arg1	lactones					121:128	BACKGROUND N-acylhomoserine lactones	93:128	BACKGROUND N-acylhomoserine lactones (AHLs)	93:135	BACKGROUND N-acylhomoserine lactones (AHLs) are well-studied signalling molecules produced by some Gram-negative Proteobacteria for bacterial cell-to-cell communication or quorum sensing.
30445965	1	96	theme	BACKGROUND	93:102	arg1	molecules					165:173	well-studied signalling molecules	141:173	well-studied signalling molecules produced by some Gram-negative Proteobacteria for bacterial cell-to-cell communication or quorum sensing	141:278	BACKGROUND N-acylhomoserine lactones (AHLs) are well-studied signalling molecules produced by some Gram-negative Proteobacteria for bacterial cell-to-cell communication or quorum sensing.
30445965	2	97	dep	Planococcus	364:374	arg1	versutus					376:383	versutus	376:383	versutus	376:383	We have previously demonstrated the degradation of AHLs by an Antarctic bacterium, Planococcus versutus L10.15T, at low temperature through the production of an AHL lactonase.
30445965	13	98	theme	sensing	1911:1917	arg1	agent					1919:1923	This anti-quorum sensing agent	1894:1923	This anti-quorum sensing agent	1894:1923	This anti-quorum sensing agent is an enzyme with low thermal stability that degrades the bacterial signalling molecules (AHLs) that are produced by many pathogens.
30445965	13	98	theme	sensing	1911:1917	arg1	enzyme					1931:1936	an enzyme	1928:1936	an enzyme with low thermal stability that degrades the bacterial signalling molecules (AHLs) that are produced by many pathogens	1928:2055	This anti-quorum sensing agent is an enzyme with low thermal stability that degrades the bacterial signalling molecules (AHLs) that are produced by many pathogens.
30445965	2	99	theme	AHL	442:444	arg1	lactonase					446:454	an AHL lactonase	439:454	an AHL lactonase	439:454	We have previously demonstrated the degradation of AHLs by an Antarctic bacterium, Planococcus versutus L10.15T, at low temperature through the production of an AHL lactonase.
30445965	5	100	theme	ester	853:857	arg1	bond					859:862	the ester bond	849:862	the ester bond of the homoserine lactone ring of AHLs	849:901	Liquid chromatography-mass spectrometry analysis confirmed that AidP functions as an AHL lactonase that hydrolyzes the ester bond of the homoserine lactone ring of AHLs.
30445965	9	101	theme	positive	1400:1407	arg1	selection					1409:1417	episodic positive selection	1391:1417	episodic positive selection of the gene in cold environments	1391:1450	branch on the phylogenetic trees also showed evidence of episodic positive selection of the gene in cold environments.
30445965	9	102	from	environments	1439:1450	arg1	evidence					1379:1386	evidence	1379:1386	evidence of episodic positive selection of the gene in cold environments	1379:1450	branch on the phylogenetic trees also showed evidence of episodic positive selection of the gene in cold environments.
30445965	6	103	theme	alignment	922:930	arg1	analysis					932:939	Multiple sequence alignment analysis	904:939	Multiple sequence alignment analysis	904:939	Multiple sequence alignment analysis and phylogenetic analysis suggested that the aidP gene encodes a novel AHL lactonase enzyme.
30445965	4	104	theme	Rapid	558:562	arg1	chromatography					582:595	Rapid resolution liquid chromatography	558:595	Rapid resolution liquid chromatography analysis	558:604	RESULTS Rapid resolution liquid chromatography analysis indicated that purified AidP possesses high AHL-degrading activity on unsubstituted, and 3-oxo substituted homoserine lactones.
30445965	14	105	theme	human	2096:2100	arg1	temperature					2107:2117	human body temperature	2096:2117	human body temperature (below 28 °C)	2096:2131	Since the enzyme is most active below human body temperature (below 28 °C), and lose its activity drastically above 32 °C, the results of a pectinolytic inhibition assay using Chinese cabbage indicated the potential of this anti-quorum sensing agent to be safely applied in the field trials.
30445965	2	106	theme	Planococcus	364:374	arg1	bacterium					353:361	an Antarctic bacterium	340:361	an Antarctic bacterium	340:361	We have previously demonstrated the degradation of AHLs by an Antarctic bacterium, Planococcus versutus L10.15T, at low temperature through the production of an AHL lactonase.
30445965	2	106	theme	Planococcus	364:374	arg1	L10.15T					385:391	Planococcus versutus L10.15T	364:391	Planococcus versutus L10.15T	364:391	We have previously demonstrated the degradation of AHLs by an Antarctic bacterium, Planococcus versutus L10.15T, at low temperature through the production of an AHL lactonase.
30445965	9	107	theme	gene	1426:1429	arg1	selection					1409:1417	episodic positive selection	1391:1417	episodic positive selection of the gene in cold environments	1391:1450	branch on the phylogenetic trees also showed evidence of episodic positive selection of the gene in cold environments.
30445965	6	108	theme	Multiple	904:911	arg1	analysis					932:939	Multiple sequence alignment analysis	904:939	Multiple sequence alignment analysis	904:939	Multiple sequence alignment analysis and phylogenetic analysis suggested that the aidP gene encodes a novel AHL lactonase enzyme.
30445965	4	109	theme	high	645:648	arg1	activity					664:671	high AHL-degrading activity	645:671	high AHL-degrading activity on unsubstituted, and 3-oxo substituted homoserine lactones	645:731	RESULTS Rapid resolution liquid chromatography analysis indicated that purified AidP possesses high AHL-degrading activity on unsubstituted, and 3-oxo substituted homoserine lactones.
30445965	13	110	theme	many	2042:2045	arg1	pathogens					2047:2055	many pathogens	2042:2055	many pathogens	2042:2055	This anti-quorum sensing agent is an enzyme with low thermal stability that degrades the bacterial signalling molecules (AHLs) that are produced by many pathogens.
30445965	3	111	theme	lactonase	490:498	arg1	gene					500:503	the AHL lactonase gene	482:503	the AHL lactonase gene	482:503	In this study, we cloned the AHL lactonase gene and characterized the purified novel enzyme.
30445965	9	112	theme	cold	1434:1437	arg1	environments					1439:1450	cold environments	1434:1450	cold environments	1434:1450	branch on the phylogenetic trees also showed evidence of episodic positive selection of the gene in cold environments.
30445965	1	113	theme	cell-to-cell	235:246	arg1	communication					248:260	bacterial cell-to-cell communication	225:260	bacterial cell-to-cell communication	225:260	BACKGROUND N-acylhomoserine lactones (AHLs) are well-studied signalling molecules produced by some Gram-negative Proteobacteria for bacterial cell-to-cell communication or quorum sensing.
30445965	11	114	theme	pathogen	1689:1696	arg1	carotovorum					1713:1723	the plant pathogen Pectobacterium carotovorum	1679:1723	the plant pathogen Pectobacterium carotovorum	1679:1723	The pectinolytic inhibition assay confirmed that this enzyme attenuated the pathogenicity of the plant pathogen Pectobacterium carotovorum in Chinese cabbage.
30445965	3	115	theme	novel	536:540	arg1	enzyme					542:547	the purified novel enzyme	523:547	the purified novel enzyme	523:547	In this study, we cloned the AHL lactonase gene and characterized the purified novel enzyme.
30962814	7	0	theme	natural	1429:1435	arg1	activity					1449:1456	natural killer cell activity	1429:1456	natural killer cell activity	1429:1456	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	7	0	theme	natural	1429:1435	arg1	responses					1311:1319	the immune-related responses	1292:1319	the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M)	1292:1529	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	10	1	theme	supplement	1867:1876	arg1	use					1830:1832	reasonable use	1819:1832	reasonable use of medicinal/edible herb-derived supplement (extract)	1819:1886	It can be further developed as new strategy for reasonable use of medicinal/edible herb-derived supplement (extract) for pharmaceutical and healthcare applications.
30962814	10	2	theme	herb-derived	1854:1865	arg1	supplement					1867:1876	medicinal/edible herb-derived supplement	1837:1876	medicinal/edible herb-derived supplement (extract)	1837:1886	It can be further developed as new strategy for reasonable use of medicinal/edible herb-derived supplement (extract) for pharmaceutical and healthcare applications.
30962814	10	2	theme	herb-derived	1854:1865	arg1	extract					1879:1885	extract	1879:1885	extract	1879:1885	It can be further developed as new strategy for reasonable use of medicinal/edible herb-derived supplement (extract) for pharmaceutical and healthcare applications.
30962814	1	3	theme	supplements	219:229	arg1	extract					147:153	BACKGROUND Water extract	130:153	BACKGROUND Water extract (WAE)	130:159	BACKGROUND Water extract (WAE) and ultrafine powder (UFP) are two types of commonly used supplements in preparing various pharmaceutical products and functional foods.
30962814	1	3	theme	supplements	219:229	arg1	powder					175:180	ultrafine powder	165:180	ultrafine powder (UFP)	165:186	BACKGROUND Water extract (WAE) and ultrafine powder (UFP) are two types of commonly used supplements in preparing various pharmaceutical products and functional foods.
30962814	1	3	theme	supplements	219:229	arg1	types					196:200	two types	192:200	two types of commonly used supplements	192:229	BACKGROUND Water extract (WAE) and ultrafine powder (UFP) are two types of commonly used supplements in preparing various pharmaceutical products and functional foods.
30962814	8	4	theme	preparations	1564:1575	arg1	potency					1536:1542	The potency	1532:1542	The potency of three Astragalus preparations on immunomodulation	1532:1595	The potency of three Astragalus preparations on immunomodulation was observed to be WIE ≥ UFP > WAE.
30962814	8	4	theme	preparations	1564:1575	arg1	WIE ≥ UFP > WAE					1616:1630	WIE ≥ UFP > WAE	1616:1630	WIE ≥ UFP > WAE	1616:1630	The potency of three Astragalus preparations on immunomodulation was observed to be WIE ≥ UFP > WAE.
30962814	10	5	theme	pharmaceutical	1892:1905	arg1	applications					1922:1933	pharmaceutical and healthcare applications	1892:1933	pharmaceutical and healthcare applications	1892:1933	It can be further developed as new strategy for reasonable use of medicinal/edible herb-derived supplement (extract) for pharmaceutical and healthcare applications.
30962814	7	6	theme	blood	1362:1366	arg1	cells					1368:1372	peripheral white blood cells	1345:1372	peripheral white blood cells	1345:1372	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	3	7	theme	solvent	641:647	arg1	method					660:665	the gradient solvent extraction method	628:665	the gradient solvent extraction method	628:665	METHODS In this study, a new whole ingredients extract of Astragalus (WIE) was prepared using the gradient solvent extraction method.
30962814	7	8	theme	spleen	1386:1391	arg1	indexes					1393:1399	spleen indexes	1386:1399	spleen indexes	1386:1399	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	7	9	theme	peripheral	1345:1354	arg1	cells					1368:1372	peripheral white blood cells	1345:1372	peripheral white blood cells	1345:1372	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	2	10	theme	many	506:509	arg1	doctors					511:517	many doctors	506:517	many doctors	506:517	However, the correlations of the chemical compositions with the traditional functions between WAE and the herb itself, as well as the potential problems of safety for UFP have been more and more concerned by many doctors and customers.
30962814	10	11	theme	healthcare	1911:1920	arg1	applications					1922:1933	pharmaceutical and healthcare applications	1892:1933	pharmaceutical and healthcare applications	1892:1933	It can be further developed as new strategy for reasonable use of medicinal/edible herb-derived supplement (extract) for pharmaceutical and healthcare applications.
30962814	7	12	dep	immunoglobulins	1506:1520	arg1	G					1522:1522	G	1522:1522	G	1522:1522	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	7	12	dep	immunoglobulins	1506:1520	arg1	immunoglobulins					1506:1520	immunoglobulins G and M	1506:1528	immunoglobulins G and M	1506:1528	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	7	12	dep	immunoglobulins	1506:1520	arg1	M					1528:1528	M	1528:1528	M	1528:1528	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	7	13	theme	consecutive	1252:1262	arg1	days					1264:1267	18 consecutive days	1249:1267	18 consecutive days	1249:1267	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	7	14	dep	responses	1311:1319	arg1	subset					1478:1483	splenic lymphocyte subset	1459:1483	splenic lymphocyte subset	1459:1483	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	7	14	dep	responses	1311:1319	arg1	weight					1327:1332	body weight	1322:1332	body weight	1322:1332	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	7	14	dep	responses	1311:1319	arg1	activity					1449:1456	natural killer cell activity	1429:1456	natural killer cell activity	1429:1456	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	7	14	dep	responses	1311:1319	arg1	levels					1496:1501	serum levels	1490:1501	serum levels	1490:1501	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	7	14	dep	responses	1311:1319	arg1	proliferations					1413:1426	splenocyte proliferations	1402:1426	splenocyte proliferations	1402:1426	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	7	14	dep	responses	1311:1319	arg1	number					1335:1340	number	1335:1340	number of peripheral white blood cells, thymus and spleen indexes	1335:1399	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	7	14	dep	responses	1311:1319	arg1	responses					1311:1319	the immune-related responses	1292:1319	the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M)	1292:1529	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	10	15	theme	new	1802:1804	arg1	strategy					1806:1813	new strategy	1802:1813	new strategy for reasonable use of medicinal/edible herb-derived supplement (extract) for pharmaceutical and healthcare applications	1802:1933	It can be further developed as new strategy for reasonable use of medicinal/edible herb-derived supplement (extract) for pharmaceutical and healthcare applications.
30962814	10	15	theme	new	1802:1804	arg1	It					1771:1772	It	1771:1772	It	1771:1772	It can be further developed as new strategy for reasonable use of medicinal/edible herb-derived supplement (extract) for pharmaceutical and healthcare applications.
30962814	5	16	theme	UFP	859:861	arg1	effect					836:841	the in vivo immunomodulatory effect	807:841	the in vivo immunomodulatory effect of WIE, WAE and UFP of Astragalus	807:875	In addition, the in vivo immunomodulatory effect of WIE, WAE and UFP of Astragalus were comprehensively compared in cyclophosphamide (Cy)-induced immunosuppressive mice.
30962814	7	17	theme	immune-related	1296:1309	arg1	activity					1449:1456	natural killer cell activity	1429:1456	natural killer cell activity	1429:1456	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	7	17	theme	immune-related	1296:1309	arg1	weight					1327:1332	body weight	1322:1332	body weight	1322:1332	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	7	17	theme	immune-related	1296:1309	arg1	number					1335:1340	number	1335:1340	number of peripheral white blood cells, thymus and spleen indexes	1335:1399	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	7	17	theme	immune-related	1296:1309	arg1	responses					1311:1319	the immune-related responses	1292:1319	the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M)	1292:1529	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	7	17	theme	immune-related	1296:1309	arg1	subset					1478:1483	splenic lymphocyte subset	1459:1483	splenic lymphocyte subset	1459:1483	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	3	18	theme	whole	563:567	arg1	extract					581:587	a new whole ingredients extract	557:587	a new whole ingredients extract of Astragalus (WIE)	557:607	METHODS In this study, a new whole ingredients extract of Astragalus (WIE) was prepared using the gradient solvent extraction method.
30962814	5	19	theme	Astragalus	866:875	arg1	effect					836:841	the in vivo immunomodulatory effect	807:841	the in vivo immunomodulatory effect of WIE, WAE and UFP of Astragalus	807:875	In addition, the in vivo immunomodulatory effect of WIE, WAE and UFP of Astragalus were comprehensively compared in cyclophosphamide (Cy)-induced immunosuppressive mice.
30962814	9	20	theme	astragalus	1694:1703	arg1	integrity					1681:1689	the chemical integrity	1668:1689	the chemical integrity of astragalus	1668:1703	CONCLUSIONS WIE maximally retained the chemical integrity of astragalus, and presented better therapeutic effectiveness than UFP and WAE.
30962814	5	21	theme	WIE	846:848	arg1	effect					836:841	the in vivo immunomodulatory effect	807:841	the in vivo immunomodulatory effect of WIE, WAE and UFP of Astragalus	807:875	In addition, the in vivo immunomodulatory effect of WIE, WAE and UFP of Astragalus were comprehensively compared in cyclophosphamide (Cy)-induced immunosuppressive mice.
30962814	2	22	theme	safety	454:459	arg1	herb					404:407	the herb	400:407	the herb itself	400:414	However, the correlations of the chemical compositions with the traditional functions between WAE and the herb itself, as well as the potential problems of safety for UFP have been more and more concerned by many doctors and customers.
30962814	2	22	theme	safety	454:459	arg1	WAE					392:394	WAE	392:394	WAE	392:394	However, the correlations of the chemical compositions with the traditional functions between WAE and the herb itself, as well as the potential problems of safety for UFP have been more and more concerned by many doctors and customers.
30962814	2	22	theme	safety	454:459	arg1	problems					442:449	the potential problems	428:449	the potential problems of safety for UFP	428:467	However, the correlations of the chemical compositions with the traditional functions between WAE and the herb itself, as well as the potential problems of safety for UFP have been more and more concerned by many doctors and customers.
30962814	9	23	dep	WIE	1645:1647	arg1	retained					1659:1666	retained	1659:1666	WIE maximally retained the chemical integrity of astragalus	1645:1703	CONCLUSIONS WIE maximally retained the chemical integrity of astragalus, and presented better therapeutic effectiveness than UFP and WAE.
30962814	5	24	theme	WAE	851:853	arg1	effect					836:841	the in vivo immunomodulatory effect	807:841	the in vivo immunomodulatory effect of WIE, WAE and UFP of Astragalus	807:875	In addition, the in vivo immunomodulatory effect of WIE, WAE and UFP of Astragalus were comprehensively compared in cyclophosphamide (Cy)-induced immunosuppressive mice.
30962814	9	25	theme	better	1720:1725	arg1	effectiveness					1739:1751	better therapeutic effectiveness	1720:1751	better therapeutic effectiveness	1720:1751	CONCLUSIONS WIE maximally retained the chemical integrity of astragalus, and presented better therapeutic effectiveness than UFP and WAE.
30962814	0	26	theme	combined	79:86	arg1	evaluation					88:97	a combined evaluation	77:97	Immunomodulatory effects of a new whole ingredients extract from Astragalus: a combined evaluation on chemistry and pharmacology.	0:128	Immunomodulatory effects of a new whole ingredients extract from Astragalus: a combined evaluation on chemistry and pharmacology.
30962814	7	27	theme	serum	1490:1494	arg1	levels					1496:1501	serum levels	1490:1501	serum levels	1490:1501	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	6	28	dep	RESULTS	964:970	arg1	determined					1084:1093	determined	1084:1093	were determined to be more abundant than those in WAE	1079:1131	RESULTS The compositions and contents of main active ingredients (polysaccharides, saponins and flavonoids) in WIE were determined to be more abundant than those in WAE.
30962814	6	29	dep	compositions	976:987	arg1	The					972:974	The	972:974	The	972:974	RESULTS The compositions and contents of main active ingredients (polysaccharides, saponins and flavonoids) in WIE were determined to be more abundant than those in WAE.
30962814	4	30	theme	chemical	672:679	arg1	compositions					681:692	The chemical compositions	668:692	The chemical compositions of WIE and WAE	668:707	The chemical compositions of WIE and WAE were comparatively analysed using spectrophotometric and chromatographic approaches.
30962814	2	31	theme	traditional	362:372	arg1	functions					374:382	the traditional functions	358:382	the traditional functions between WAE and the herb itself, as well as the potential problems of safety for UFP	358:467	However, the correlations of the chemical compositions with the traditional functions between WAE and the herb itself, as well as the potential problems of safety for UFP have been more and more concerned by many doctors and customers.
30962814	0	32	theme	Immunomodulatory	0:15	arg1	effects					17:23	Immunomodulatory effects	0:23	Immunomodulatory effects of a new whole ingredients extract from Astragalus: a combined evaluation on chemistry and pharmacology.	0:128	Immunomodulatory effects of a new whole ingredients extract from Astragalus: a combined evaluation on chemistry and pharmacology.
30962814	1	33	theme	various	244:250	arg1	products					267:274	various pharmaceutical products	244:274	various pharmaceutical products	244:274	BACKGROUND Water extract (WAE) and ultrafine powder (UFP) are two types of commonly used supplements in preparing various pharmaceutical products and functional foods.
30962814	6	34	theme	ingredients	1017:1027	arg1	contents					993:1000	contents	993:1000	contents	993:1000	RESULTS The compositions and contents of main active ingredients (polysaccharides, saponins and flavonoids) in WIE were determined to be more abundant than those in WAE.
30962814	6	34	theme	ingredients	1017:1027	arg1	compositions					976:987	compositions	976:987	compositions	976:987	RESULTS The compositions and contents of main active ingredients (polysaccharides, saponins and flavonoids) in WIE were determined to be more abundant than those in WAE.
30962814	6	35	theme	main	1005:1008	arg1	ingredients					1017:1027	main active ingredients	1005:1027	main active ingredients (polysaccharides, saponins and flavonoids)	1005:1070	RESULTS The compositions and contents of main active ingredients (polysaccharides, saponins and flavonoids) in WIE were determined to be more abundant than those in WAE.
30962814	3	36	theme	Astragalus	592:601	arg1	extract					581:587	a new whole ingredients extract	557:587	a new whole ingredients extract of Astragalus (WIE)	557:607	METHODS In this study, a new whole ingredients extract of Astragalus (WIE) was prepared using the gradient solvent extraction method.
30962814	4	37	theme	chromatographic	766:780	arg1	approaches					782:791	spectrophotometric and chromatographic approaches	743:791	spectrophotometric and chromatographic approaches	743:791	The chemical compositions of WIE and WAE were comparatively analysed using spectrophotometric and chromatographic approaches.
30962814	5	38	theme	in	811:812	arg1	effect					836:841	the in vivo immunomodulatory effect	807:841	the in vivo immunomodulatory effect of WIE, WAE and UFP of Astragalus	807:875	In addition, the in vivo immunomodulatory effect of WIE, WAE and UFP of Astragalus were comprehensively compared in cyclophosphamide (Cy)-induced immunosuppressive mice.
30962814	1	39	theme	functional	280:289	arg1	foods					291:295	functional foods	280:295	functional foods	280:295	BACKGROUND Water extract (WAE) and ultrafine powder (UFP) are two types of commonly used supplements in preparing various pharmaceutical products and functional foods.
30962814	0	40	theme	new	30:32	arg1	extract					52:58	a new whole ingredients extract	28:58	a new whole ingredients extract	28:58	Immunomodulatory effects of a new whole ingredients extract from Astragalus: a combined evaluation on chemistry and pharmacology.
30962814	7	41	theme	splenic	1459:1465	arg1	responses					1311:1319	the immune-related responses	1292:1319	the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M)	1292:1529	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	7	41	theme	splenic	1459:1465	arg1	subset					1478:1483	splenic lymphocyte subset	1459:1483	splenic lymphocyte subset	1459:1483	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	5	42	dep	in	811:812	arg1	vivo					814:817	vivo	814:817	vivo	814:817	In addition, the in vivo immunomodulatory effect of WIE, WAE and UFP of Astragalus were comprehensively compared in cyclophosphamide (Cy)-induced immunosuppressive mice.
30962814	10	43	link	herb-derived	1854:1865	arg1	supplement					1867:1876	medicinal/edible herb-derived supplement	1837:1876	medicinal/edible herb-derived supplement (extract)	1837:1886	It can be further developed as new strategy for reasonable use of medicinal/edible herb-derived supplement (extract) for pharmaceutical and healthcare applications.
30962814	10	43	link	herb-derived	1854:1865	arg1	extract					1879:1885	extract	1879:1885	extract	1879:1885	It can be further developed as new strategy for reasonable use of medicinal/edible herb-derived supplement (extract) for pharmaceutical and healthcare applications.
30962814	0	44	theme	ingredients	40:50	arg1	extract					52:58	a new whole ingredients extract	28:58	a new whole ingredients extract	28:58	Immunomodulatory effects of a new whole ingredients extract from Astragalus: a combined evaluation on chemistry and pharmacology.
30962814	7	45	theme	killer	1437:1442	arg1	activity					1449:1456	natural killer cell activity	1429:1456	natural killer cell activity	1429:1456	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	7	45	theme	killer	1437:1442	arg1	responses					1311:1319	the immune-related responses	1292:1319	the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M)	1292:1529	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	5	46	theme	immunosuppressive	940:956	arg1	mice					958:961	cyclophosphamide (Cy)-induced immunosuppressive mice	910:961	cyclophosphamide (Cy)-induced immunosuppressive mice	910:961	In addition, the in vivo immunomodulatory effect of WIE, WAE and UFP of Astragalus were comprehensively compared in cyclophosphamide (Cy)-induced immunosuppressive mice.
30962814	4	47	theme	spectrophotometric	743:760	arg1	approaches					782:791	spectrophotometric and chromatographic approaches	743:791	spectrophotometric and chromatographic approaches	743:791	The chemical compositions of WIE and WAE were comparatively analysed using spectrophotometric and chromatographic approaches.
30962814	3	48	theme	gradient	632:639	arg1	method					660:665	the gradient solvent extraction method	628:665	the gradient solvent extraction method	628:665	METHODS In this study, a new whole ingredients extract of Astragalus (WIE) was prepared using the gradient solvent extraction method.
30962814	2	49	theme	chemical	331:338	arg1	compositions					340:351	the chemical compositions	327:351	the chemical compositions	327:351	However, the correlations of the chemical compositions with the traditional functions between WAE and the herb itself, as well as the potential problems of safety for UFP have been more and more concerned by many doctors and customers.
30962814	1	50	theme	used	214:217	arg1	supplements					219:229	commonly used supplements	205:229	commonly used supplements	205:229	BACKGROUND Water extract (WAE) and ultrafine powder (UFP) are two types of commonly used supplements in preparing various pharmaceutical products and functional foods.
30962814	7	51	theme	splenocyte	1402:1411	arg1	proliferations					1413:1426	splenocyte proliferations	1402:1426	splenocyte proliferations	1402:1426	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	7	52	theme	low	1195:1197	arg1	dosage					1199:1204	low dosage	1195:1204	low dosage of WIE (equalled to 1.0 g herb/kg/day)	1195:1243	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	10	53	theme	medicinal/edible	1837:1852	arg1	supplement					1867:1876	medicinal/edible herb-derived supplement	1837:1876	medicinal/edible herb-derived supplement (extract)	1837:1886	It can be further developed as new strategy for reasonable use of medicinal/edible herb-derived supplement (extract) for pharmaceutical and healthcare applications.
30962814	10	53	theme	medicinal/edible	1837:1852	arg1	extract					1879:1885	extract	1879:1885	extract	1879:1885	It can be further developed as new strategy for reasonable use of medicinal/edible herb-derived supplement (extract) for pharmaceutical and healthcare applications.
30962814	7	54	theme	indexes	1393:1399	arg1	subset					1478:1483	splenic lymphocyte subset	1459:1483	splenic lymphocyte subset	1459:1483	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	7	54	theme	indexes	1393:1399	arg1	weight					1327:1332	body weight	1322:1332	body weight	1322:1332	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	7	54	theme	indexes	1393:1399	arg1	activity					1449:1456	natural killer cell activity	1429:1456	natural killer cell activity	1429:1456	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	7	54	theme	indexes	1393:1399	arg1	levels					1496:1501	serum levels	1490:1501	serum levels	1490:1501	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	7	54	theme	indexes	1393:1399	arg1	proliferations					1413:1426	splenocyte proliferations	1402:1426	splenocyte proliferations	1402:1426	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	7	54	theme	indexes	1393:1399	arg1	number					1335:1340	number	1335:1340	number of peripheral white blood cells, thymus and spleen indexes	1335:1399	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	7	54	theme	indexes	1393:1399	arg1	responses					1311:1319	the immune-related responses	1292:1319	the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M)	1292:1529	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	7	55	theme	thymus	1375:1380	arg1	subset					1478:1483	splenic lymphocyte subset	1459:1483	splenic lymphocyte subset	1459:1483	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	7	55	theme	thymus	1375:1380	arg1	weight					1327:1332	body weight	1322:1332	body weight	1322:1332	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	7	55	theme	thymus	1375:1380	arg1	activity					1449:1456	natural killer cell activity	1429:1456	natural killer cell activity	1429:1456	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	7	55	theme	thymus	1375:1380	arg1	levels					1496:1501	serum levels	1490:1501	serum levels	1490:1501	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	7	55	theme	thymus	1375:1380	arg1	proliferations					1413:1426	splenocyte proliferations	1402:1426	splenocyte proliferations	1402:1426	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	7	55	theme	thymus	1375:1380	arg1	number					1335:1340	number	1335:1340	number of peripheral white blood cells, thymus and spleen indexes	1335:1399	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	7	55	theme	thymus	1375:1380	arg1	responses					1311:1319	the immune-related responses	1292:1319	the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M)	1292:1529	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	8	56	theme	Astragalus	1553:1562	arg1	preparations					1564:1575	three Astragalus preparations	1547:1575	three Astragalus preparations	1547:1575	The potency of three Astragalus preparations on immunomodulation was observed to be WIE ≥ UFP > WAE.
30962814	5	57	theme	immunomodulatory	819:834	arg1	effect					836:841	the in vivo immunomodulatory effect	807:841	the in vivo immunomodulatory effect of WIE, WAE and UFP of Astragalus	807:875	In addition, the in vivo immunomodulatory effect of WIE, WAE and UFP of Astragalus were comprehensively compared in cyclophosphamide (Cy)-induced immunosuppressive mice.
30962814	3	58	dep	METHODS	534:540	arg1	study					550:554	this study	545:554	this study	545:554	METHODS In this study, a new whole ingredients extract of Astragalus (WIE) was prepared using the gradient solvent extraction method.
30962814	7	59	theme	cells	1368:1372	arg1	subset					1478:1483	splenic lymphocyte subset	1459:1483	splenic lymphocyte subset	1459:1483	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	7	59	theme	cells	1368:1372	arg1	weight					1327:1332	body weight	1322:1332	body weight	1322:1332	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	7	59	theme	cells	1368:1372	arg1	activity					1449:1456	natural killer cell activity	1429:1456	natural killer cell activity	1429:1456	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	7	59	theme	cells	1368:1372	arg1	levels					1496:1501	serum levels	1490:1501	serum levels	1490:1501	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	7	59	theme	cells	1368:1372	arg1	proliferations					1413:1426	splenocyte proliferations	1402:1426	splenocyte proliferations	1402:1426	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	7	59	theme	cells	1368:1372	arg1	number					1335:1340	number	1335:1340	number of peripheral white blood cells, thymus and spleen indexes	1335:1399	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	7	59	theme	cells	1368:1372	arg1	responses					1311:1319	the immune-related responses	1292:1319	the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M)	1292:1529	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	3	60	theme	extraction	649:658	arg1	method					660:665	the gradient solvent extraction method	628:665	the gradient solvent extraction method	628:665	METHODS In this study, a new whole ingredients extract of Astragalus (WIE) was prepared using the gradient solvent extraction method.
30962814	6	61	from	contents	993:1000	arg1	WIE					1075:1077	WIE	1075:1077	WIE	1075:1077	RESULTS The compositions and contents of main active ingredients (polysaccharides, saponins and flavonoids) in WIE were determined to be more abundant than those in WAE.
30962814	1	62	theme	ultrafine	165:173	arg1	UFP					183:185	UFP	183:185	UFP	183:185	BACKGROUND Water extract (WAE) and ultrafine powder (UFP) are two types of commonly used supplements in preparing various pharmaceutical products and functional foods.
30962814	1	62	theme	ultrafine	165:173	arg1	powder					175:180	ultrafine powder	165:180	ultrafine powder (UFP)	165:186	BACKGROUND Water extract (WAE) and ultrafine powder (UFP) are two types of commonly used supplements in preparing various pharmaceutical products and functional foods.
30962814	1	62	theme	ultrafine	165:173	arg1	types					196:200	two types	192:200	two types of commonly used supplements	192:229	BACKGROUND Water extract (WAE) and ultrafine powder (UFP) are two types of commonly used supplements in preparing various pharmaceutical products and functional foods.
30962814	1	62	theme	ultrafine	165:173	arg1	extract					147:153	BACKGROUND Water extract	130:153	BACKGROUND Water extract (WAE)	130:159	BACKGROUND Water extract (WAE) and ultrafine powder (UFP) are two types of commonly used supplements in preparing various pharmaceutical products and functional foods.
30962814	7	63	theme	white	1356:1360	arg1	cells					1368:1372	peripheral white blood cells	1345:1372	peripheral white blood cells	1345:1372	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	8	64	from	potency	1536:1542	arg1	immunomodulation					1580:1595	immunomodulation	1580:1595	immunomodulation	1580:1595	The potency of three Astragalus preparations on immunomodulation was observed to be WIE ≥ UFP > WAE.
30962814	0	65	from	Astragalus	65:74	arg1	effects					17:23	Immunomodulatory effects	0:23	Immunomodulatory effects of a new whole ingredients extract from Astragalus: a combined evaluation on chemistry and pharmacology.	0:128	Immunomodulatory effects of a new whole ingredients extract from Astragalus: a combined evaluation on chemistry and pharmacology.
30962814	1	66	theme	BACKGROUND	130:139	arg1	extract					147:153	BACKGROUND Water extract	130:153	BACKGROUND Water extract (WAE)	130:159	BACKGROUND Water extract (WAE) and ultrafine powder (UFP) are two types of commonly used supplements in preparing various pharmaceutical products and functional foods.
30962814	1	66	theme	BACKGROUND	130:139	arg1	powder					175:180	ultrafine powder	165:180	ultrafine powder (UFP)	165:186	BACKGROUND Water extract (WAE) and ultrafine powder (UFP) are two types of commonly used supplements in preparing various pharmaceutical products and functional foods.
30962814	1	66	theme	BACKGROUND	130:139	arg1	types					196:200	two types	192:200	two types of commonly used supplements	192:229	BACKGROUND Water extract (WAE) and ultrafine powder (UFP) are two types of commonly used supplements in preparing various pharmaceutical products and functional foods.
30962814	1	66	theme	BACKGROUND	130:139	arg1	WAE					156:158	WAE	156:158	WAE	156:158	BACKGROUND Water extract (WAE) and ultrafine powder (UFP) are two types of commonly used supplements in preparing various pharmaceutical products and functional foods.
30962814	9	67	theme	chemical	1672:1679	arg1	integrity					1681:1689	the chemical integrity	1668:1689	the chemical integrity of astragalus	1668:1703	CONCLUSIONS WIE maximally retained the chemical integrity of astragalus, and presented better therapeutic effectiveness than UFP and WAE.
30962814	7	68	theme	body	1322:1325	arg1	responses					1311:1319	the immune-related responses	1292:1319	the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M)	1292:1529	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	7	68	theme	body	1322:1325	arg1	weight					1327:1332	body weight	1322:1332	body weight	1322:1332	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	5	69	theme	-induced	931:938	arg1	mice					958:961	cyclophosphamide (Cy)-induced immunosuppressive mice	910:961	cyclophosphamide (Cy)-induced immunosuppressive mice	910:961	In addition, the in vivo immunomodulatory effect of WIE, WAE and UFP of Astragalus were comprehensively compared in cyclophosphamide (Cy)-induced immunosuppressive mice.
30962814	3	70	theme	new	559:561	arg1	extract					581:587	a new whole ingredients extract	557:587	a new whole ingredients extract of Astragalus (WIE)	557:607	METHODS In this study, a new whole ingredients extract of Astragalus (WIE) was prepared using the gradient solvent extraction method.
30962814	0	71	from	evaluation	88:97	arg1	pharmacology					116:127	pharmacology	116:127	pharmacology	116:127	Immunomodulatory effects of a new whole ingredients extract from Astragalus: a combined evaluation on chemistry and pharmacology.
30962814	0	71	from	evaluation	88:97	arg1	chemistry					102:110	chemistry	102:110	chemistry	102:110	Immunomodulatory effects of a new whole ingredients extract from Astragalus: a combined evaluation on chemistry and pharmacology.
30962814	1	72	theme	Water	141:145	arg1	extract					147:153	BACKGROUND Water extract	130:153	BACKGROUND Water extract (WAE)	130:159	BACKGROUND Water extract (WAE) and ultrafine powder (UFP) are two types of commonly used supplements in preparing various pharmaceutical products and functional foods.
30962814	1	72	theme	Water	141:145	arg1	powder					175:180	ultrafine powder	165:180	ultrafine powder (UFP)	165:186	BACKGROUND Water extract (WAE) and ultrafine powder (UFP) are two types of commonly used supplements in preparing various pharmaceutical products and functional foods.
30962814	1	72	theme	Water	141:145	arg1	types					196:200	two types	192:200	two types of commonly used supplements	192:229	BACKGROUND Water extract (WAE) and ultrafine powder (UFP) are two types of commonly used supplements in preparing various pharmaceutical products and functional foods.
30962814	1	72	theme	Water	141:145	arg1	WAE					156:158	WAE	156:158	WAE	156:158	BACKGROUND Water extract (WAE) and ultrafine powder (UFP) are two types of commonly used supplements in preparing various pharmaceutical products and functional foods.
30962814	7	73	theme	immunoglobulins	1506:1520	arg1	subset					1478:1483	splenic lymphocyte subset	1459:1483	splenic lymphocyte subset	1459:1483	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	7	73	theme	immunoglobulins	1506:1520	arg1	weight					1327:1332	body weight	1322:1332	body weight	1322:1332	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	7	73	theme	immunoglobulins	1506:1520	arg1	activity					1449:1456	natural killer cell activity	1429:1456	natural killer cell activity	1429:1456	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	7	73	theme	immunoglobulins	1506:1520	arg1	levels					1496:1501	serum levels	1490:1501	serum levels	1490:1501	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	7	73	theme	immunoglobulins	1506:1520	arg1	proliferations					1413:1426	splenocyte proliferations	1402:1426	splenocyte proliferations	1402:1426	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	7	73	theme	immunoglobulins	1506:1520	arg1	number					1335:1340	number	1335:1340	number of peripheral white blood cells, thymus and spleen indexes	1335:1399	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	7	73	theme	immunoglobulins	1506:1520	arg1	responses					1311:1319	the immune-related responses	1292:1319	the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M)	1292:1529	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	7	74	theme	immunosuppressive	1148:1164	arg1	mice					1166:1169	Cy-induced immunosuppressive mice	1137:1169	Cy-induced immunosuppressive mice	1137:1169	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	7	75	theme	Cy-induced	1137:1146	arg1	mice					1166:1169	Cy-induced immunosuppressive mice	1137:1169	Cy-induced immunosuppressive mice	1137:1169	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	9	76	theme	therapeutic	1727:1737	arg1	effectiveness					1739:1751	better therapeutic effectiveness	1720:1751	better therapeutic effectiveness	1720:1751	CONCLUSIONS WIE maximally retained the chemical integrity of astragalus, and presented better therapeutic effectiveness than UFP and WAE.
30962814	6	77	from	compositions	976:987	arg1	WIE					1075:1077	WIE	1075:1077	WIE	1075:1077	RESULTS The compositions and contents of main active ingredients (polysaccharides, saponins and flavonoids) in WIE were determined to be more abundant than those in WAE.
30962814	4	78	theme	WIE	697:699	arg1	compositions					681:692	The chemical compositions	668:692	The chemical compositions of WIE and WAE	668:707	The chemical compositions of WIE and WAE were comparatively analysed using spectrophotometric and chromatographic approaches.
30962814	4	79	theme	WAE	705:707	arg1	compositions					681:692	The chemical compositions	668:692	The chemical compositions of WIE and WAE	668:707	The chemical compositions of WIE and WAE were comparatively analysed using spectrophotometric and chromatographic approaches.
30962814	6	80	dep	ingredients	1017:1027	arg1	flavonoids					1060:1069	flavonoids	1060:1069	flavonoids	1060:1069	RESULTS The compositions and contents of main active ingredients (polysaccharides, saponins and flavonoids) in WIE were determined to be more abundant than those in WAE.
30962814	6	80	dep	ingredients	1017:1027	arg1	saponins					1047:1054	saponins	1047:1054	saponins	1047:1054	RESULTS The compositions and contents of main active ingredients (polysaccharides, saponins and flavonoids) in WIE were determined to be more abundant than those in WAE.
30962814	6	80	dep	ingredients	1017:1027	arg1	polysaccharides					1030:1044	polysaccharides	1030:1044	polysaccharides	1030:1044	RESULTS The compositions and contents of main active ingredients (polysaccharides, saponins and flavonoids) in WIE were determined to be more abundant than those in WAE.
30962814	1	81	theme	pharmaceutical	252:265	arg1	products					267:274	various pharmaceutical products	244:274	various pharmaceutical products	244:274	BACKGROUND Water extract (WAE) and ultrafine powder (UFP) are two types of commonly used supplements in preparing various pharmaceutical products and functional foods.
30962814	6	82	theme	active	1010:1015	arg1	ingredients					1017:1027	main active ingredients	1005:1027	main active ingredients (polysaccharides, saponins and flavonoids)	1005:1070	RESULTS The compositions and contents of main active ingredients (polysaccharides, saponins and flavonoids) in WIE were determined to be more abundant than those in WAE.
30962814	0	83	theme	whole	34:38	arg1	extract					52:58	a new whole ingredients extract	28:58	a new whole ingredients extract	28:58	Immunomodulatory effects of a new whole ingredients extract from Astragalus: a combined evaluation on chemistry and pharmacology.
30962814	2	84	theme	potential	432:440	arg1	problems					442:449	the potential problems	428:449	the potential problems of safety for UFP	428:467	However, the correlations of the chemical compositions with the traditional functions between WAE and the herb itself, as well as the potential problems of safety for UFP have been more and more concerned by many doctors and customers.
30962814	3	85	theme	ingredients	569:579	arg1	extract					581:587	a new whole ingredients extract	557:587	a new whole ingredients extract of Astragalus (WIE)	557:607	METHODS In this study, a new whole ingredients extract of Astragalus (WIE) was prepared using the gradient solvent extraction method.
30962814	0	86	theme	extract	52:58	arg1	effects					17:23	Immunomodulatory effects	0:23	Immunomodulatory effects of a new whole ingredients extract from Astragalus: a combined evaluation on chemistry and pharmacology.	0:128	Immunomodulatory effects of a new whole ingredients extract from Astragalus: a combined evaluation on chemistry and pharmacology.
30962814	7	87	theme	lymphocyte	1467:1476	arg1	responses					1311:1319	the immune-related responses	1292:1319	the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M)	1292:1529	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	7	87	theme	lymphocyte	1467:1476	arg1	subset					1478:1483	splenic lymphocyte subset	1459:1483	splenic lymphocyte subset	1459:1483	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	7	88	dep	1.0 g	1226:1230	arg1	to					1223:1224	to	1223:1224	to	1223:1224	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	7	89	theme	WIE	1209:1211	arg1	dosage					1199:1204	low dosage	1195:1204	low dosage of WIE (equalled to 1.0 g herb/kg/day)	1195:1243	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	10	90	theme	reasonable	1819:1828	arg1	use					1830:1832	reasonable use	1819:1832	reasonable use of medicinal/edible herb-derived supplement (extract)	1819:1886	It can be further developed as new strategy for reasonable use of medicinal/edible herb-derived supplement (extract) for pharmaceutical and healthcare applications.
30962814	7	91	theme	cell	1444:1447	arg1	activity					1449:1456	natural killer cell activity	1429:1456	natural killer cell activity	1429:1456	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	7	91	theme	cell	1444:1447	arg1	responses					1311:1319	the immune-related responses	1292:1319	the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M)	1292:1529	In Cy-induced immunosuppressive mice, oral administered with low dosage of WIE (equalled to 1.0 g herb/kg/day) for 18 consecutive days significantly improved the immune-related responses (body weight, number of peripheral white blood cells, thymus and spleen indexes, splenocyte proliferations, natural killer cell activity, splenic lymphocyte subset, and serum levels of immunoglobulins G and M).
30962814	0	92	dep	effects	17:23	arg1	evaluation					88:97	a combined evaluation	77:97	Immunomodulatory effects of a new whole ingredients extract from Astragalus: a combined evaluation on chemistry and pharmacology.	0:128	Immunomodulatory effects of a new whole ingredients extract from Astragalus: a combined evaluation on chemistry and pharmacology.
30962814	2	93	with	correlations	311:322	arg1	functions					374:382	the traditional functions	358:382	the traditional functions between WAE and the herb itself, as well as the potential problems of safety for UFP	358:467	However, the correlations of the chemical compositions with the traditional functions between WAE and the herb itself, as well as the potential problems of safety for UFP have been more and more concerned by many doctors and customers.
30962814	2	94	theme	compositions	340:351	arg1	correlations					311:322	the correlations	307:322	the correlations of the chemical compositions with the traditional functions between WAE and the herb itself, as well as the potential problems of safety for UFP	307:467	However, the correlations of the chemical compositions with the traditional functions between WAE and the herb itself, as well as the potential problems of safety for UFP have been more and more concerned by many doctors and customers.
30810517	12	0	theme	hybridization	1133:1145	arg1	data					1147:1150	The physiological and DNA-DNA hybridization data	1103:1150	The physiological and DNA-DNA hybridization data	1103:1150	The physiological and DNA-DNA hybridization data allowed the differentiation of strain HSS6-8T from its related species.
30810517	5	1	theme	cell-wall	395:403	arg1	acid					413:416	the cell-wall muramic acid	391:416	the cell-wall muramic acid	391:416	The acyl type of the cell-wall muramic acid was glycolyl.
30810517	15	2	theme	type	1369:1372	arg1	HSS6-8T					1384:1390	HSS6-8T	1384:1390	HSS6-8T (=TBRC 8927T=JCM 17126T)	1384:1415	The type strain is HSS6-8T (=TBRC 8927T=JCM 17126T).
30810517	15	2	theme	type	1369:1372	arg1	strain					1374:1379	The type strain	1365:1379	The type strain	1365:1379	The type strain is HSS6-8T (=TBRC 8927T=JCM 17126T).
30810517	8	3	theme	major	694:698	arg1	MK-10					718:722	MK-10	718:722	MK-10	718:722	The major menaquinones were MK-10(H6) and MK-10(H4).
30810517	8	3	theme	major	694:698	arg1	menaquinones					700:711	The major menaquinones	690:711	The major menaquinones	690:711	The major menaquinones were MK-10(H6) and MK-10(H4).
30810517	15	4	theme	8927T=JCM	1399:1407	arg1	HSS6-8T					1384:1390	HSS6-8T	1384:1390	HSS6-8T (=TBRC 8927T=JCM 17126T)	1384:1415	The type strain is HSS6-8T (=TBRC 8927T=JCM 17126T).
30810517	15	4	theme	8927T=JCM	1399:1407	arg1	17126T					1409:1414	=TBRC 8927T=JCM 17126T	1393:1414	=TBRC 8927T=JCM 17126T	1393:1414	The type strain is HSS6-8T (=TBRC 8927T=JCM 17126T).
30810517	11	5	theme	Micromonospora	972:985	arg1	%					1010:1010	98.2 %	1005:1010	98.2 %	1005:1010	16S rRNA gene sequence analysis revealed that strain HSS6-8T was closely related to Micromonospora nigra DSM 43818T (98.2 %), Micromonospora eburnea DSM 44814T (98.2 %) and Micromonospora spongicola S3-1T (98.1 %).
30810517	11	5	theme	Micromonospora	972:985	arg1	DSM					993:995	Micromonospora nigra DSM 43818T	972:1002	Micromonospora nigra DSM 43818T (98.2 %)	972:1011	16S rRNA gene sequence analysis revealed that strain HSS6-8T was closely related to Micromonospora nigra DSM 43818T (98.2 %), Micromonospora eburnea DSM 44814T (98.2 %) and Micromonospora spongicola S3-1T (98.1 %).
30810517	5	6	theme	muramic	405:411	arg1	acid					413:416	the cell-wall muramic acid	391:416	the cell-wall muramic acid	391:416	The acyl type of the cell-wall muramic acid was glycolyl.
30810517	3	7	from	classification	244:257	arg1	Micromonospora					272:285	the genus Micromonospora	262:285	the genus Micromonospora	262:285	The strain had the chemotaxonomic properties consistent with its classification in the genus Micromonospora.
30810517	15	8	theme	=TBRC	1393:1397	arg1	HSS6-8T					1384:1390	HSS6-8T	1384:1390	HSS6-8T (=TBRC 8927T=JCM 17126T)	1384:1415	The type strain is HSS6-8T (=TBRC 8927T=JCM 17126T).
30810517	15	8	theme	=TBRC	1393:1397	arg1	17126T					1409:1414	=TBRC 8927T=JCM 17126T	1393:1414	=TBRC 8927T=JCM 17126T	1393:1414	The type strain is HSS6-8T (=TBRC 8927T=JCM 17126T).
30810517	11	9	theme	DSM	1037:1039	arg1	44814T					1041:1046	Micromonospora eburnea DSM 44814T	1014:1046	Micromonospora eburnea DSM 44814T (98.2 %)	1014:1055	16S rRNA gene sequence analysis revealed that strain HSS6-8T was closely related to Micromonospora nigra DSM 43818T (98.2 %), Micromonospora eburnea DSM 44814T (98.2 %) and Micromonospora spongicola S3-1T (98.1 %).
30810517	11	9	theme	DSM	1037:1039	arg1	%					1054:1054	98.2 %	1049:1054	98.2 %	1049:1054	16S rRNA gene sequence analysis revealed that strain HSS6-8T was closely related to Micromonospora nigra DSM 43818T (98.2 %), Micromonospora eburnea DSM 44814T (98.2 %) and Micromonospora spongicola S3-1T (98.1 %).
30810517	1	10	theme	hot	51:53	arg1	soil					62:65	hot spring soil	51:65	hot spring soil	51:65	nov., isolated from hot spring soil.
30810517	5	11	theme	acid	413:416	arg1	glycolyl					422:429	glycolyl	422:429	glycolyl	422:429	The acyl type of the cell-wall muramic acid was glycolyl.
30810517	5	11	theme	acid	413:416	arg1	type					383:386	The acyl type	374:386	The acyl type of the cell-wall muramic acid	374:416	The acyl type of the cell-wall muramic acid was glycolyl.
30810517	10	12	theme	genomic	861:867	arg1	DNA					869:871	the genomic DNA	857:871	the genomic DNA	857:871	The G+C content of the genomic DNA was 70.5 mol%.
30810517	1	13	theme	spring	55:60	arg1	soil					62:65	hot spring soil	51:65	hot spring soil	51:65	nov., isolated from hot spring soil.
30810517	4	14	contain	have	312:315	arg1	strain					292:297	The strain	288:297	The strain	288:297	The strain was found to have meso-diaminopimelic acid in the cell-wall peptidoglycan.
30810517	4	14	contain	have	312:315	arg2	acid					337:340	meso-diaminopimelic acid	317:340	meso-diaminopimelic acid	317:340	The strain was found to have meso-diaminopimelic acid in the cell-wall peptidoglycan.
30810517	3	15	theme	consistent	224:233	arg1	properties					213:222	the chemotaxonomic properties	194:222	the chemotaxonomic properties consistent with its classification in the genus Micromonospora	194:285	The strain had the chemotaxonomic properties consistent with its classification in the genus Micromonospora.
30810517	12	16	theme	related	1207:1213	arg1	species					1215:1221	its related species	1203:1221	its related species	1203:1221	The physiological and DNA-DNA hybridization data allowed the differentiation of strain HSS6-8T from its related species.
30810517	6	17	from	sugars	445:450	arg1	hydrolysates					464:475	the cell hydrolysates	455:475	the cell hydrolysates	455:475	The reducing sugars in the cell hydrolysates were glucose, arabinose, xylose, ribose, mannose, galactose and rhamnose.
30810517	4	18	theme	cell-wall	349:357	arg1	peptidoglycan					359:371	the cell-wall peptidoglycan	345:371	the cell-wall peptidoglycan	345:371	The strain was found to have meso-diaminopimelic acid in the cell-wall peptidoglycan.
30810517	12	19	dep	physiological	1107:1119	arg1	The					1103:1105	The	1103:1105	The	1103:1105	The physiological and DNA-DNA hybridization data allowed the differentiation of strain HSS6-8T from its related species.
30810517	13	20	theme	genus	1275:1279	arg1	Micromonospora					1281:1294	the genus Micromonospora	1271:1294	the genus Micromonospora	1271:1294	Thus, the strain represents a novel species of the genus Micromonospora, for which the name Micromonospora caldifontis sp.
30810517	0	21	theme	caldifontis	15:25	arg1	sp					27:28	Micromonospora caldifontis sp	0:28	Micromonospora caldifontis sp.	0:29	Micromonospora caldifontis sp.
30810517	3	22	theme	chemotaxonomic	198:211	arg1	properties					213:222	the chemotaxonomic properties	194:222	the chemotaxonomic properties consistent with its classification in the genus Micromonospora	194:285	The strain had the chemotaxonomic properties consistent with its classification in the genus Micromonospora.
30810517	0	23	theme	Micromonospora	0:13	arg1	sp					27:28	Micromonospora caldifontis sp	0:28	Micromonospora caldifontis sp.	0:29	Micromonospora caldifontis sp.
30810517	11	24	theme	gene	897:900	arg1	analysis					911:918	16S rRNA gene sequence analysis	888:918	16S rRNA gene sequence analysis	888:918	16S rRNA gene sequence analysis revealed that strain HSS6-8T was closely related to Micromonospora nigra DSM 43818T (98.2 %), Micromonospora eburnea DSM 44814T (98.2 %) and Micromonospora spongicola S3-1T (98.1 %).
30810517	2	25	theme	hot	162:164	arg1	soil					173:176	hot spring soil	162:176	hot spring soil	162:176	A single spore forming actinomycete, designated strain HSS6-8T, was isolated from a sample of hot spring soil.
30810517	9	26	dep	 0	788:789	arg1	anteiso-C15 					821:832	anteiso-C15 	821:832	anteiso-C15 	821:832	The major cellular fatty acids were iso-C16 : 0, anteiso-C17 : 0, C17 : 0 and anteiso-C15 : 0.
30810517	9	26	dep	 0	788:789	arg1	 0					814:815	 0	814:815	 0	814:815	The major cellular fatty acids were iso-C16 : 0, anteiso-C17 : 0, C17 : 0 and anteiso-C15 : 0.
30810517	9	26	dep	 0	788:789	arg1	C17 					809:812	C17 	809:812	C17 	809:812	The major cellular fatty acids were iso-C16 : 0, anteiso-C17 : 0, C17 : 0 and anteiso-C15 : 0.
30810517	9	26	dep	 0	788:789	arg1	 0					834:835	 0	834:835	 0	834:835	The major cellular fatty acids were iso-C16 : 0, anteiso-C17 : 0, C17 : 0 and anteiso-C15 : 0.
30810517	11	27	theme	sequence	902:909	arg1	analysis					911:918	16S rRNA gene sequence analysis	888:918	16S rRNA gene sequence analysis	888:918	16S rRNA gene sequence analysis revealed that strain HSS6-8T was closely related to Micromonospora nigra DSM 43818T (98.2 %), Micromonospora eburnea DSM 44814T (98.2 %) and Micromonospora spongicola S3-1T (98.1 %).
30810517	10	28	theme	70.5 mol	877:884	arg1	%					885:885	70.5 mol%	877:885	70.5 mol%	877:885	The G+C content of the genomic DNA was 70.5 mol%.
30810517	10	28	theme	70.5 mol	877:884	arg1	G+C content					842:852	The G+C content	838:852	The G+C content of the genomic DNA	838:871	The G+C content of the genomic DNA was 70.5 mol%.
30810517	9	29	dep	iso-C16 	779:786	arg1	anteiso-C17 					792:803	anteiso-C17 	792:803	anteiso-C17 	792:803	The major cellular fatty acids were iso-C16 : 0, anteiso-C17 : 0, C17 : 0 and anteiso-C15 : 0.
30810517	9	29	dep	iso-C16 	779:786	arg1	 0					788:789	 0	788:789	 0	788:789	The major cellular fatty acids were iso-C16 : 0, anteiso-C17 : 0, C17 : 0 and anteiso-C15 : 0.
30810517	11	30	theme	16S	888:890	arg1	rRNA					892:895	16S rRNA	888:895	16S rRNA gene sequence analysis	888:918	16S rRNA gene sequence analysis revealed that strain HSS6-8T was closely related to Micromonospora nigra DSM 43818T (98.2 %), Micromonospora eburnea DSM 44814T (98.2 %) and Micromonospora spongicola S3-1T (98.1 %).
30810517	12	31	theme	DNA-DNA	1125:1131	arg1	hybridization					1133:1145	DNA-DNA hybridization	1125:1145	DNA-DNA hybridization	1125:1145	The physiological and DNA-DNA hybridization data allowed the differentiation of strain HSS6-8T from its related species.
30810517	11	32	theme	eburnea	1029:1035	arg1	44814T					1041:1046	Micromonospora eburnea DSM 44814T	1014:1046	Micromonospora eburnea DSM 44814T (98.2 %)	1014:1055	16S rRNA gene sequence analysis revealed that strain HSS6-8T was closely related to Micromonospora nigra DSM 43818T (98.2 %), Micromonospora eburnea DSM 44814T (98.2 %) and Micromonospora spongicola S3-1T (98.1 %).
30810517	11	32	theme	eburnea	1029:1035	arg1	%					1054:1054	98.2 %	1049:1054	98.2 %	1049:1054	16S rRNA gene sequence analysis revealed that strain HSS6-8T was closely related to Micromonospora nigra DSM 43818T (98.2 %), Micromonospora eburnea DSM 44814T (98.2 %) and Micromonospora spongicola S3-1T (98.1 %).
30810517	13	33	theme	caldifontis	1331:1341	arg1	sp					1343:1344	the name Micromonospora caldifontis sp	1307:1344	the name Micromonospora caldifontis sp	1307:1344	Thus, the strain represents a novel species of the genus Micromonospora, for which the name Micromonospora caldifontis sp.
30810517	11	34	theme	rRNA	892:895	arg1	analysis					911:918	16S rRNA gene sequence analysis	888:918	16S rRNA gene sequence analysis	888:918	16S rRNA gene sequence analysis revealed that strain HSS6-8T was closely related to Micromonospora nigra DSM 43818T (98.2 %), Micromonospora eburnea DSM 44814T (98.2 %) and Micromonospora spongicola S3-1T (98.1 %).
30810517	2	35	theme	forming	83:89	arg1	actinomycete					91:102	A single spore forming actinomycete	68:102	A single spore forming actinomycete	68:102	A single spore forming actinomycete, designated strain HSS6-8T, was isolated from a sample of hot spring soil.
30810517	6	36	theme	cell	459:462	arg1	hydrolysates					464:475	the cell hydrolysates	455:475	the cell hydrolysates	455:475	The reducing sugars in the cell hydrolysates were glucose, arabinose, xylose, ribose, mannose, galactose and rhamnose.
30810517	9	37	theme	major	747:751	arg1	iso-C16 					779:786	iso-C16 	779:786	iso-C16 	779:786	The major cellular fatty acids were iso-C16 : 0, anteiso-C17 : 0, C17 : 0 and anteiso-C15 : 0.
30810517	9	37	theme	major	747:751	arg1	acids					768:772	The major cellular fatty acids	743:772	The major cellular fatty acids	743:772	The major cellular fatty acids were iso-C16 : 0, anteiso-C17 : 0, C17 : 0 and anteiso-C15 : 0.
30810517	4	38	theme	meso-diaminopimelic	317:335	arg1	acid					337:340	meso-diaminopimelic acid	317:340	meso-diaminopimelic acid	317:340	The strain was found to have meso-diaminopimelic acid in the cell-wall peptidoglycan.
30810517	2	39	theme	spore	77:81	arg1	actinomycete					91:102	A single spore forming actinomycete	68:102	A single spore forming actinomycete	68:102	A single spore forming actinomycete, designated strain HSS6-8T, was isolated from a sample of hot spring soil.
30810517	9	40	theme	cellular	753:760	arg1	iso-C16 					779:786	iso-C16 	779:786	iso-C16 	779:786	The major cellular fatty acids were iso-C16 : 0, anteiso-C17 : 0, C17 : 0 and anteiso-C15 : 0.
30810517	9	40	theme	cellular	753:760	arg1	acids					768:772	The major cellular fatty acids	743:772	The major cellular fatty acids	743:772	The major cellular fatty acids were iso-C16 : 0, anteiso-C17 : 0, C17 : 0 and anteiso-C15 : 0.
30810517	2	41	theme	single	70:75	arg1	actinomycete					91:102	A single spore forming actinomycete	68:102	A single spore forming actinomycete	68:102	A single spore forming actinomycete, designated strain HSS6-8T, was isolated from a sample of hot spring soil.
30810517	9	42	theme	fatty	762:766	arg1	iso-C16 					779:786	iso-C16 	779:786	iso-C16 	779:786	The major cellular fatty acids were iso-C16 : 0, anteiso-C17 : 0, C17 : 0 and anteiso-C15 : 0.
30810517	9	42	theme	fatty	762:766	arg1	acids					768:772	The major cellular fatty acids	743:772	The major cellular fatty acids	743:772	The major cellular fatty acids were iso-C16 : 0, anteiso-C17 : 0, C17 : 0 and anteiso-C15 : 0.
30810517	2	43	attach	isolated	136:143	arg1	sample					152:157	a sample	150:157	a sample of hot spring soil	150:176	A single spore forming actinomycete, designated strain HSS6-8T, was isolated from a sample of hot spring soil.
30810517	2	43	attach	isolated	136:143	arg2	actinomycete					91:102	A single spore forming actinomycete	68:102	A single spore forming actinomycete	68:102	A single spore forming actinomycete, designated strain HSS6-8T, was isolated from a sample of hot spring soil.
30810517	13	44	theme	Micromonospora	1281:1294	arg1	species					1260:1266	a novel species	1252:1266	a novel species	1252:1266	Thus, the strain represents a novel species of the genus Micromonospora, for which the name Micromonospora caldifontis sp.
30810517	3	45	theme	genus	266:270	arg1	Micromonospora					272:285	the genus Micromonospora	262:285	the genus Micromonospora	262:285	The strain had the chemotaxonomic properties consistent with its classification in the genus Micromonospora.
30810517	2	46	theme	soil	173:176	arg1	sample					152:157	a sample	150:157	a sample of hot spring soil	150:176	A single spore forming actinomycete, designated strain HSS6-8T, was isolated from a sample of hot spring soil.
30810517	12	47	from	species	1215:1221	arg1	differentiation					1164:1178	the differentiation	1160:1178	the differentiation of strain HSS6-8T from its related species	1160:1221	The physiological and DNA-DNA hybridization data allowed the differentiation of strain HSS6-8T from its related species.
30810517	3	48	with	consistent	224:233	arg1	classification					244:257	its classification	240:257	its classification in the genus Micromonospora	240:285	The strain had the chemotaxonomic properties consistent with its classification in the genus Micromonospora.
30810517	6	49	theme	reducing	436:443	arg1	sugars					445:450	The reducing sugars	432:450	The reducing sugars in the cell hydrolysates	432:475	The reducing sugars in the cell hydrolysates were glucose, arabinose, xylose, ribose, mannose, galactose and rhamnose.
30810517	6	49	theme	reducing	436:443	arg1	glucose					482:488	glucose	482:488	glucose	482:488	The reducing sugars in the cell hydrolysates were glucose, arabinose, xylose, ribose, mannose, galactose and rhamnose.
30810517	2	50	theme	spring	166:171	arg1	soil					173:176	hot spring soil	162:176	hot spring soil	162:176	A single spore forming actinomycete, designated strain HSS6-8T, was isolated from a sample of hot spring soil.
30810517	10	51	theme	DNA	869:871	arg1	%					885:885	70.5 mol%	877:885	70.5 mol%	877:885	The G+C content of the genomic DNA was 70.5 mol%.
30810517	10	51	theme	DNA	869:871	arg1	G+C content					842:852	The G+C content	838:852	The G+C content of the genomic DNA	838:871	The G+C content of the genomic DNA was 70.5 mol%.
30810517	13	52	theme	Micromonospora	1316:1329	arg1	sp					1343:1344	the name Micromonospora caldifontis sp	1307:1344	the name Micromonospora caldifontis sp	1307:1344	Thus, the strain represents a novel species of the genus Micromonospora, for which the name Micromonospora caldifontis sp.
30810517	5	53	theme	acyl	378:381	arg1	glycolyl					422:429	glycolyl	422:429	glycolyl	422:429	The acyl type of the cell-wall muramic acid was glycolyl.
30810517	5	53	theme	acyl	378:381	arg1	type					383:386	The acyl type	374:386	The acyl type of the cell-wall muramic acid	374:416	The acyl type of the cell-wall muramic acid was glycolyl.
30810517	3	54	contain	had	190:192	arg2	properties					213:222	the chemotaxonomic properties	194:222	the chemotaxonomic properties consistent with its classification in the genus Micromonospora	194:285	The strain had the chemotaxonomic properties consistent with its classification in the genus Micromonospora.
30810517	3	54	contain	had	190:192	arg1	strain					183:188	The strain	179:188	The strain	179:188	The strain had the chemotaxonomic properties consistent with its classification in the genus Micromonospora.
30810517	11	55	theme	Micromonospora	1014:1027	arg1	44814T					1041:1046	Micromonospora eburnea DSM 44814T	1014:1046	Micromonospora eburnea DSM 44814T (98.2 %)	1014:1055	16S rRNA gene sequence analysis revealed that strain HSS6-8T was closely related to Micromonospora nigra DSM 43818T (98.2 %), Micromonospora eburnea DSM 44814T (98.2 %) and Micromonospora spongicola S3-1T (98.1 %).
30810517	11	55	theme	Micromonospora	1014:1027	arg1	%					1054:1054	98.2 %	1049:1054	98.2 %	1049:1054	16S rRNA gene sequence analysis revealed that strain HSS6-8T was closely related to Micromonospora nigra DSM 43818T (98.2 %), Micromonospora eburnea DSM 44814T (98.2 %) and Micromonospora spongicola S3-1T (98.1 %).
30810517	11	56	theme	nigra	987:991	arg1	%					1010:1010	98.2 %	1005:1010	98.2 %	1005:1010	16S rRNA gene sequence analysis revealed that strain HSS6-8T was closely related to Micromonospora nigra DSM 43818T (98.2 %), Micromonospora eburnea DSM 44814T (98.2 %) and Micromonospora spongicola S3-1T (98.1 %).
30810517	11	56	theme	nigra	987:991	arg1	DSM					993:995	Micromonospora nigra DSM 43818T	972:1002	Micromonospora nigra DSM 43818T (98.2 %)	972:1011	16S rRNA gene sequence analysis revealed that strain HSS6-8T was closely related to Micromonospora nigra DSM 43818T (98.2 %), Micromonospora eburnea DSM 44814T (98.2 %) and Micromonospora spongicola S3-1T (98.1 %).
30810517	12	57	theme	strain	1183:1188	arg1	differentiation					1164:1178	the differentiation	1160:1178	the differentiation of strain HSS6-8T from its related species	1160:1221	The physiological and DNA-DNA hybridization data allowed the differentiation of strain HSS6-8T from its related species.
30810517	13	58	theme	novel	1254:1258	arg1	species					1260:1266	a novel species	1252:1266	a novel species	1252:1266	Thus, the strain represents a novel species of the genus Micromonospora, for which the name Micromonospora caldifontis sp.
30810517	2	59	theme	strain	116:121	arg1	HSS6-8T					123:129	strain HSS6-8T	116:129	strain HSS6-8T	116:129	A single spore forming actinomycete, designated strain HSS6-8T, was isolated from a sample of hot spring soil.
30810517	13	60	theme	name	1311:1314	arg1	sp					1343:1344	the name Micromonospora caldifontis sp	1307:1344	the name Micromonospora caldifontis sp	1307:1344	Thus, the strain represents a novel species of the genus Micromonospora, for which the name Micromonospora caldifontis sp.
30810517	12	61	theme	physiological	1107:1119	arg1	data					1147:1150	The physiological and DNA-DNA hybridization data	1103:1150	The physiological and DNA-DNA hybridization data	1103:1150	The physiological and DNA-DNA hybridization data allowed the differentiation of strain HSS6-8T from its related species.
30810517	11	62	theme	strain	934:939	arg1	HSS6-8T					941:947	strain HSS6-8T	934:947	strain HSS6-8T	934:947	16S rRNA gene sequence analysis revealed that strain HSS6-8T was closely related to Micromonospora nigra DSM 43818T (98.2 %), Micromonospora eburnea DSM 44814T (98.2 %) and Micromonospora spongicola S3-1T (98.1 %).
29680483	2	0	theme	fibroin	481:487	arg1	films					489:493	fibroin films	481:493	fibroin films	481:493	NT-loaded SF films composition shows predominance of β-sheet structures that conferred adequate mechanical properties, transparency, moderate roughness and low swelling index to fibroin films.
29680483	7	1	theme	gram-positive	1243:1255	arg1	microorganisms					1257:1270	gram-positive microorganisms	1243:1270	gram-positive microorganisms	1243:1270	In addition, anodic iontophoresis presented a bacteriostatic effect against gram-positive microorganisms.
29680483	3	2	theme	Infrared	496:503	arg1	spectroscopy					505:516	Infrared spectroscopy	496:516	Infrared spectroscopy	496:516	Infrared spectroscopy and thermal analysis suggested the presence of non-covalent interactions between NT and fibroin.
29680483	0	3	theme	wound	77:81	arg1	healing					83:89	wound healing	77:89	wound healing	77:89	Iontophoresis-stimulated silk fibroin films as a peptide delivery system for wound healing.
29680483	8	4	dep	0.6 mA/cm2	1320:1329	arg1	to					1317:1318	to	1317:1318	to	1317:1318	Different iontophoresis densities, from 0.2 to 0.6 mA/cm2, did not significantly reduce fibroblast viability after 30 min of application.
29680483	5	5	dep	E.	813:814	arg1	coli					816:819	coli	816:819	coli	816:819	In vitro studies in E. coli liposaccharide-stimulated macrophages showed a significant reduction of interleukins production after NT-loaded film application, whereas NT solution did not reduce them.
29680483	5	6	theme	NT-loaded	923:931	arg1	application					938:948	NT-loaded film application	923:948	NT-loaded film application	923:948	In vitro studies in E. coli liposaccharide-stimulated macrophages showed a significant reduction of interleukins production after NT-loaded film application, whereas NT solution did not reduce them.
29680483	8	7	theme	Different	1273:1281	arg1	densities					1297:1305	Different iontophoresis densities	1273:1305	Different iontophoresis densities	1273:1305	Different iontophoresis densities, from 0.2 to 0.6 mA/cm2, did not significantly reduce fibroblast viability after 30 min of application.
29680483	1	8	theme	inflammatory	222:233	arg1	process					235:241	the inflammatory process	218:241	the inflammatory process	218:241	Silk fibroin (SF) films containing a peptide, neurotensin (NT), stimulated by iontophoresis were developed aiming to modulate the inflammatory process and prevent the growth of microorganisms typical of wounds.
29680483	5	9	theme	production	906:915	arg1	reduction					880:888	a significant reduction	866:888	a significant reduction of interleukins production	866:915	In vitro studies in E. coli liposaccharide-stimulated macrophages showed a significant reduction of interleukins production after NT-loaded film application, whereas NT solution did not reduce them.
29680483	6	10	contain	containing	1029:1038	arg1	films					1023:1027	Bi-laminated NT-loaded fibroin films	992:1027	Bi-laminated NT-loaded fibroin films containing silver electrodes	992:1056	Bi-laminated NT-loaded fibroin films containing silver electrodes provided a burst release of NT when anodic iontophoresis was applied, enabling a rapid onset of drug action.
29680483	6	10	contain	containing	1029:1038	arg2	electrodes					1047:1056	silver electrodes	1040:1056	silver electrodes	1040:1056	Bi-laminated NT-loaded fibroin films containing silver electrodes provided a burst release of NT when anodic iontophoresis was applied, enabling a rapid onset of drug action.
29680483	6	11	theme	fibroin	1015:1021	arg1	films					1023:1027	Bi-laminated NT-loaded fibroin films	992:1027	Bi-laminated NT-loaded fibroin films containing silver electrodes	992:1056	Bi-laminated NT-loaded fibroin films containing silver electrodes provided a burst release of NT when anodic iontophoresis was applied, enabling a rapid onset of drug action.
29680483	5	12	theme	NT	959:960	arg1	solution					962:969	NT solution	959:969	NT solution	959:969	In vitro studies in E. coli liposaccharide-stimulated macrophages showed a significant reduction of interleukins production after NT-loaded film application, whereas NT solution did not reduce them.
29680483	6	13	theme	NT-loaded	1005:1013	arg1	films					1023:1027	Bi-laminated NT-loaded fibroin films	992:1027	Bi-laminated NT-loaded fibroin films containing silver electrodes	992:1056	Bi-laminated NT-loaded fibroin films containing silver electrodes provided a burst release of NT when anodic iontophoresis was applied, enabling a rapid onset of drug action.
29680483	4	14	theme	prolonged	759:767	arg1	release					769:775	its prolonged release	755:775	its prolonged release for up to 72 h	755:790	Using the MALDI imaging technique, it was possible to visualize the homogeneous NT distribution throughout the film surface, in addition to its prolonged release for up to 72 h.
29680483	6	15	theme	rapid	1139:1143	arg1	onset					1145:1149	a rapid onset	1137:1149	a rapid onset of drug action	1137:1164	Bi-laminated NT-loaded fibroin films containing silver electrodes provided a burst release of NT when anodic iontophoresis was applied, enabling a rapid onset of drug action.
29680483	4	16	theme	film	726:729	arg1	surface					731:737	the film surface	722:737	the film surface	722:737	Using the MALDI imaging technique, it was possible to visualize the homogeneous NT distribution throughout the film surface, in addition to its prolonged release for up to 72 h.
29680483	7	17	theme	anodic	1180:1185	arg1	iontophoresis					1187:1199	anodic iontophoresis	1180:1199	anodic iontophoresis	1180:1199	In addition, anodic iontophoresis presented a bacteriostatic effect against gram-positive microorganisms.
29680483	5	18	theme	liposaccharide-stimulated	821:845	arg1	macrophages					847:857	E. coli liposaccharide-stimulated macrophages	813:857	E. coli liposaccharide-stimulated macrophages	813:857	In vitro studies in E. coli liposaccharide-stimulated macrophages showed a significant reduction of interleukins production after NT-loaded film application, whereas NT solution did not reduce them.
29680483	4	19	dep	72 h	787:790	arg1	to					784:785	to	784:785	to	784:785	Using the MALDI imaging technique, it was possible to visualize the homogeneous NT distribution throughout the film surface, in addition to its prolonged release for up to 72 h.
29680483	5	20	theme	In	793:794	arg1	studies					802:808	In vitro studies	793:808	In vitro studies in E. coli liposaccharide-stimulated macrophages	793:857	In vitro studies in E. coli liposaccharide-stimulated macrophages showed a significant reduction of interleukins production after NT-loaded film application, whereas NT solution did not reduce them.
29680483	5	21	dep	In	793:794	arg1	vitro					796:800	vitro	796:800	vitro	796:800	In vitro studies in E. coli liposaccharide-stimulated macrophages showed a significant reduction of interleukins production after NT-loaded film application, whereas NT solution did not reduce them.
29680483	7	22	attach	presented	1201:1209	arg1	addition					1170:1177	addition	1170:1177	addition	1170:1177	In addition, anodic iontophoresis presented a bacteriostatic effect against gram-positive microorganisms.
29680483	7	22	attach	presented	1201:1209	arg2	iontophoresis					1187:1199	anodic iontophoresis	1180:1199	anodic iontophoresis	1180:1199	In addition, anodic iontophoresis presented a bacteriostatic effect against gram-positive microorganisms.
29680483	0	23	theme	Iontophoresis-stimulated	0:23	arg1	silk					25:28	Iontophoresis-stimulated silk	0:28	Iontophoresis-stimulated silk	0:28	Iontophoresis-stimulated silk fibroin films as a peptide delivery system for wound healing.
29680483	5	24	theme	significant	868:878	arg1	reduction					880:888	a significant reduction	866:888	a significant reduction of interleukins production	866:915	In vitro studies in E. coli liposaccharide-stimulated macrophages showed a significant reduction of interleukins production after NT-loaded film application, whereas NT solution did not reduce them.
29680483	5	25	from	studies	802:808	arg1	macrophages					847:857	E. coli liposaccharide-stimulated macrophages	813:857	E. coli liposaccharide-stimulated macrophages	813:857	In vitro studies in E. coli liposaccharide-stimulated macrophages showed a significant reduction of interleukins production after NT-loaded film application, whereas NT solution did not reduce them.
29680483	3	26	theme	interactions	578:589	arg1	presence					553:560	the presence	549:560	the presence of non-covalent interactions between NT and fibroin	549:612	Infrared spectroscopy and thermal analysis suggested the presence of non-covalent interactions between NT and fibroin.
29680483	6	27	theme	anodic	1094:1099	arg1	iontophoresis					1101:1113	anodic iontophoresis	1094:1113	anodic iontophoresis	1094:1113	Bi-laminated NT-loaded fibroin films containing silver electrodes provided a burst release of NT when anodic iontophoresis was applied, enabling a rapid onset of drug action.
29680483	2	28	theme	structures	364:373	arg1	predominance					340:351	predominance	340:351	predominance of β-sheet structures that conferred adequate mechanical properties, transparency, moderate roughness and low swelling index to fibroin films	340:493	NT-loaded SF films composition shows predominance of β-sheet structures that conferred adequate mechanical properties, transparency, moderate roughness and low swelling index to fibroin films.
29680483	2	29	theme	moderate	436:443	arg1	roughness					445:453	moderate roughness	436:453	moderate roughness	436:453	NT-loaded SF films composition shows predominance of β-sheet structures that conferred adequate mechanical properties, transparency, moderate roughness and low swelling index to fibroin films.
29680483	6	30	theme	Bi-laminated	992:1003	arg1	films					1023:1027	Bi-laminated NT-loaded fibroin films	992:1027	Bi-laminated NT-loaded fibroin films containing silver electrodes	992:1056	Bi-laminated NT-loaded fibroin films containing silver electrodes provided a burst release of NT when anodic iontophoresis was applied, enabling a rapid onset of drug action.
29680483	1	31	theme	Silk	92:95	arg1	SF					106:107	SF	106:107	SF	106:107	Silk fibroin (SF) films containing a peptide, neurotensin (NT), stimulated by iontophoresis were developed aiming to modulate the inflammatory process and prevent the growth of microorganisms typical of wounds.
29680483	1	31	theme	Silk	92:95	arg1	fibroin					97:103	Silk fibroin	92:103	Silk fibroin (SF) films containing a peptide, neurotensin (NT), stimulated by iontophoresis	92:182	Silk fibroin (SF) films containing a peptide, neurotensin (NT), stimulated by iontophoresis were developed aiming to modulate the inflammatory process and prevent the growth of microorganisms typical of wounds.
29680483	2	32	theme	β-sheet	356:362	arg1	structures					364:373	β-sheet structures	356:373	β-sheet structures that conferred adequate mechanical properties, transparency, moderate roughness and low swelling index to fibroin films	356:493	NT-loaded SF films composition shows predominance of β-sheet structures that conferred adequate mechanical properties, transparency, moderate roughness and low swelling index to fibroin films.
29680483	9	33	from	platform	1501:1508	arg1	conclusion					1414:1423	conclusion	1414:1423	conclusion	1414:1423	In conclusion, iontophoretic-stimulated peptide-loaded fibroin films could be a promising platform for the treatment of wounds.
29680483	6	34	theme	action	1159:1164	arg1	onset					1145:1149	a rapid onset	1137:1149	a rapid onset of drug action	1137:1164	Bi-laminated NT-loaded fibroin films containing silver electrodes provided a burst release of NT when anodic iontophoresis was applied, enabling a rapid onset of drug action.
29680483	1	35	theme	fibroin	97:103	arg1	films					110:114	Silk fibroin (SF) films	92:114	Silk fibroin (SF) films containing a peptide, neurotensin (NT), stimulated by iontophoresis	92:182	Silk fibroin (SF) films containing a peptide, neurotensin (NT), stimulated by iontophoresis were developed aiming to modulate the inflammatory process and prevent the growth of microorganisms typical of wounds.
29680483	9	36	theme	peptide-loaded	1451:1464	arg1	platform					1501:1508	a promising platform	1489:1508	a promising platform for the treatment of wounds	1489:1536	In conclusion, iontophoretic-stimulated peptide-loaded fibroin films could be a promising platform for the treatment of wounds.
29680483	9	36	theme	peptide-loaded	1451:1464	arg1	films					1474:1478	iontophoretic-stimulated peptide-loaded fibroin films	1426:1478	iontophoretic-stimulated peptide-loaded fibroin films	1426:1478	In conclusion, iontophoretic-stimulated peptide-loaded fibroin films could be a promising platform for the treatment of wounds.
29680483	6	37	theme	NT	1086:1087	arg1	release					1075:1081	a burst release	1067:1081	a burst release of NT	1067:1087	Bi-laminated NT-loaded fibroin films containing silver electrodes provided a burst release of NT when anodic iontophoresis was applied, enabling a rapid onset of drug action.
29680483	5	38	theme	E.	813:814	arg1	macrophages					847:857	E. coli liposaccharide-stimulated macrophages	813:857	E. coli liposaccharide-stimulated macrophages	813:857	In vitro studies in E. coli liposaccharide-stimulated macrophages showed a significant reduction of interleukins production after NT-loaded film application, whereas NT solution did not reduce them.
29680483	3	39	theme	non-covalent	565:576	arg1	interactions					578:589	non-covalent interactions	565:589	non-covalent interactions between NT and fibroin	565:612	Infrared spectroscopy and thermal analysis suggested the presence of non-covalent interactions between NT and fibroin.
29680483	5	40	theme	interleukins	893:904	arg1	production					906:915	interleukins production	893:915	interleukins production	893:915	In vitro studies in E. coli liposaccharide-stimulated macrophages showed a significant reduction of interleukins production after NT-loaded film application, whereas NT solution did not reduce them.
29680483	4	41	dep	release	769:775	arg1	addition					743:750	addition	743:750	addition	743:750	Using the MALDI imaging technique, it was possible to visualize the homogeneous NT distribution throughout the film surface, in addition to its prolonged release for up to 72 h.
29680483	9	42	theme	wounds	1531:1536	arg1	treatment					1518:1526	the treatment	1514:1526	the treatment of wounds	1514:1536	In conclusion, iontophoretic-stimulated peptide-loaded fibroin films could be a promising platform for the treatment of wounds.
29680483	2	43	theme	mechanical	399:408	arg1	properties					410:419	adequate mechanical properties	390:419	adequate mechanical properties	390:419	NT-loaded SF films composition shows predominance of β-sheet structures that conferred adequate mechanical properties, transparency, moderate roughness and low swelling index to fibroin films.
29680483	2	44	theme	swelling	463:470	arg1	index					472:476	low swelling index	459:476	low swelling index	459:476	NT-loaded SF films composition shows predominance of β-sheet structures that conferred adequate mechanical properties, transparency, moderate roughness and low swelling index to fibroin films.
29680483	1	45	theme	microorganisms	269:282	arg1	growth					259:264	the growth	255:264	the growth of microorganisms typical of wounds	255:300	Silk fibroin (SF) films containing a peptide, neurotensin (NT), stimulated by iontophoresis were developed aiming to modulate the inflammatory process and prevent the growth of microorganisms typical of wounds.
29680483	9	46	theme	promising	1491:1499	arg1	platform					1501:1508	a promising platform	1489:1508	a promising platform for the treatment of wounds	1489:1536	In conclusion, iontophoretic-stimulated peptide-loaded fibroin films could be a promising platform for the treatment of wounds.
29680483	9	46	theme	promising	1491:1499	arg1	films					1474:1478	iontophoretic-stimulated peptide-loaded fibroin films	1426:1478	iontophoretic-stimulated peptide-loaded fibroin films	1426:1478	In conclusion, iontophoretic-stimulated peptide-loaded fibroin films could be a promising platform for the treatment of wounds.
29680483	8	47	theme	fibroblast	1361:1370	arg1	viability					1372:1380	fibroblast viability	1361:1380	fibroblast viability	1361:1380	Different iontophoresis densities, from 0.2 to 0.6 mA/cm2, did not significantly reduce fibroblast viability after 30 min of application.
29680483	2	48	theme	adequate	390:397	arg1	properties					410:419	adequate mechanical properties	390:419	adequate mechanical properties	390:419	NT-loaded SF films composition shows predominance of β-sheet structures that conferred adequate mechanical properties, transparency, moderate roughness and low swelling index to fibroin films.
29680483	0	49	theme	delivery	57:64	arg1	system					66:71	a peptide delivery system	47:71	a peptide delivery system for wound healing	47:89	Iontophoresis-stimulated silk fibroin films as a peptide delivery system for wound healing.
29680483	0	49	theme	delivery	57:64	arg1	films					38:42	films	38:42	films	38:42	Iontophoresis-stimulated silk fibroin films as a peptide delivery system for wound healing.
29680483	1	50	theme	typical	284:290	arg1	microorganisms					269:282	microorganisms	269:282	microorganisms typical of wounds	269:300	Silk fibroin (SF) films containing a peptide, neurotensin (NT), stimulated by iontophoresis were developed aiming to modulate the inflammatory process and prevent the growth of microorganisms typical of wounds.
29680483	2	51	theme	films	316:320	arg1	composition					322:332	NT-loaded SF films composition	303:332	NT-loaded SF films composition	303:332	NT-loaded SF films composition shows predominance of β-sheet structures that conferred adequate mechanical properties, transparency, moderate roughness and low swelling index to fibroin films.
29680483	0	52	theme	peptide	49:55	arg1	system					66:71	a peptide delivery system	47:71	a peptide delivery system for wound healing	47:89	Iontophoresis-stimulated silk fibroin films as a peptide delivery system for wound healing.
29680483	0	52	theme	peptide	49:55	arg1	films					38:42	films	38:42	films	38:42	Iontophoresis-stimulated silk fibroin films as a peptide delivery system for wound healing.
29680483	9	53	theme	iontophoretic-stimulated	1426:1449	arg1	platform					1501:1508	a promising platform	1489:1508	a promising platform for the treatment of wounds	1489:1536	In conclusion, iontophoretic-stimulated peptide-loaded fibroin films could be a promising platform for the treatment of wounds.
29680483	9	53	theme	iontophoretic-stimulated	1426:1449	arg1	films					1474:1478	iontophoretic-stimulated peptide-loaded fibroin films	1426:1478	iontophoretic-stimulated peptide-loaded fibroin films	1426:1478	In conclusion, iontophoretic-stimulated peptide-loaded fibroin films could be a promising platform for the treatment of wounds.
29680483	7	54	theme	bacteriostatic	1213:1226	arg1	effect					1228:1233	a bacteriostatic effect	1211:1233	a bacteriostatic effect against gram-positive microorganisms	1211:1270	In addition, anodic iontophoresis presented a bacteriostatic effect against gram-positive microorganisms.
29680483	2	55	theme	SF	313:314	arg1	composition					322:332	NT-loaded SF films composition	303:332	NT-loaded SF films composition	303:332	NT-loaded SF films composition shows predominance of β-sheet structures that conferred adequate mechanical properties, transparency, moderate roughness and low swelling index to fibroin films.
29680483	1	56	theme	wounds	295:300	arg1	typical					284:290	typical	284:290	typical	284:290	Silk fibroin (SF) films containing a peptide, neurotensin (NT), stimulated by iontophoresis were developed aiming to modulate the inflammatory process and prevent the growth of microorganisms typical of wounds.
29680483	2	57	theme	NT-loaded	303:311	arg1	composition					322:332	NT-loaded SF films composition	303:332	NT-loaded SF films composition	303:332	NT-loaded SF films composition shows predominance of β-sheet structures that conferred adequate mechanical properties, transparency, moderate roughness and low swelling index to fibroin films.
29680483	9	58	theme	fibroin	1466:1472	arg1	platform					1501:1508	a promising platform	1489:1508	a promising platform for the treatment of wounds	1489:1536	In conclusion, iontophoretic-stimulated peptide-loaded fibroin films could be a promising platform for the treatment of wounds.
29680483	9	58	theme	fibroin	1466:1472	arg1	films					1474:1478	iontophoretic-stimulated peptide-loaded fibroin films	1426:1478	iontophoretic-stimulated peptide-loaded fibroin films	1426:1478	In conclusion, iontophoretic-stimulated peptide-loaded fibroin films could be a promising platform for the treatment of wounds.
29680483	5	59	theme	film	933:936	arg1	application					938:948	NT-loaded film application	923:948	NT-loaded film application	923:948	In vitro studies in E. coli liposaccharide-stimulated macrophages showed a significant reduction of interleukins production after NT-loaded film application, whereas NT solution did not reduce them.
29680483	8	60	theme	application	1398:1408	arg1	30 min					1388:1393	30 min	1388:1393	30 min of application	1388:1408	Different iontophoresis densities, from 0.2 to 0.6 mA/cm2, did not significantly reduce fibroblast viability after 30 min of application.
29680483	4	61	theme	homogeneous	683:693	arg1	distribution					698:709	the homogeneous NT distribution	679:709	the homogeneous NT distribution	679:709	Using the MALDI imaging technique, it was possible to visualize the homogeneous NT distribution throughout the film surface, in addition to its prolonged release for up to 72 h.
29680483	6	62	theme	drug	1154:1157	arg1	action					1159:1164	drug action	1154:1164	drug action	1154:1164	Bi-laminated NT-loaded fibroin films containing silver electrodes provided a burst release of NT when anodic iontophoresis was applied, enabling a rapid onset of drug action.
29680483	6	63	theme	burst	1069:1073	arg1	release					1075:1081	a burst release	1067:1081	a burst release of NT	1067:1087	Bi-laminated NT-loaded fibroin films containing silver electrodes provided a burst release of NT when anodic iontophoresis was applied, enabling a rapid onset of drug action.
29680483	8	64	from	0.6 mA/cm2	1320:1329	arg1	densities					1297:1305	Different iontophoresis densities	1273:1305	Different iontophoresis densities	1273:1305	Different iontophoresis densities, from 0.2 to 0.6 mA/cm2, did not significantly reduce fibroblast viability after 30 min of application.
29680483	4	65	theme	NT	695:696	arg1	distribution					698:709	the homogeneous NT distribution	679:709	the homogeneous NT distribution	679:709	Using the MALDI imaging technique, it was possible to visualize the homogeneous NT distribution throughout the film surface, in addition to its prolonged release for up to 72 h.
29680483	8	66	theme	iontophoresis	1283:1295	arg1	densities					1297:1305	Different iontophoresis densities	1273:1305	Different iontophoresis densities	1273:1305	Different iontophoresis densities, from 0.2 to 0.6 mA/cm2, did not significantly reduce fibroblast viability after 30 min of application.
29680483	4	67	theme	imaging	631:637	arg1	technique					639:647	the MALDI imaging technique	621:647	the MALDI imaging technique	621:647	Using the MALDI imaging technique, it was possible to visualize the homogeneous NT distribution throughout the film surface, in addition to its prolonged release for up to 72 h.
29680483	2	68	theme	low	459:461	arg1	index					472:476	low swelling index	459:476	low swelling index	459:476	NT-loaded SF films composition shows predominance of β-sheet structures that conferred adequate mechanical properties, transparency, moderate roughness and low swelling index to fibroin films.
29680483	1	69	contain	containing	116:125	arg1	films					110:114	Silk fibroin (SF) films	92:114	Silk fibroin (SF) films containing a peptide, neurotensin (NT), stimulated by iontophoresis	92:182	Silk fibroin (SF) films containing a peptide, neurotensin (NT), stimulated by iontophoresis were developed aiming to modulate the inflammatory process and prevent the growth of microorganisms typical of wounds.
29680483	1	69	contain	containing	116:125	arg2	neurotensin					138:148	neurotensin	138:148	neurotensin (NT)	138:153	Silk fibroin (SF) films containing a peptide, neurotensin (NT), stimulated by iontophoresis were developed aiming to modulate the inflammatory process and prevent the growth of microorganisms typical of wounds.
29680483	1	69	contain	containing	116:125	arg2	peptide					129:135	a peptide	127:135	a peptide	127:135	Silk fibroin (SF) films containing a peptide, neurotensin (NT), stimulated by iontophoresis were developed aiming to modulate the inflammatory process and prevent the growth of microorganisms typical of wounds.
29680483	6	70	theme	silver	1040:1045	arg1	electrodes					1047:1056	silver electrodes	1040:1056	silver electrodes	1040:1056	Bi-laminated NT-loaded fibroin films containing silver electrodes provided a burst release of NT when anodic iontophoresis was applied, enabling a rapid onset of drug action.
29680483	3	71	theme	thermal	522:528	arg1	analysis					530:537	thermal analysis	522:537	thermal analysis	522:537	Infrared spectroscopy and thermal analysis suggested the presence of non-covalent interactions between NT and fibroin.
29680483	4	72	theme	MALDI	625:629	arg1	technique					639:647	the MALDI imaging technique	621:647	the MALDI imaging technique	621:647	Using the MALDI imaging technique, it was possible to visualize the homogeneous NT distribution throughout the film surface, in addition to its prolonged release for up to 72 h.
29428970	7	0	theme	Amycolatopsis	1385:1397	arg1	sp					1410:1411	the name Amycolatopsis vastitatis sp	1376:1411	the name Amycolatopsis vastitatis sp	1376:1411	Consequently, the strain is considered to represent a novel species of Amycolatopsis for which the name Amycolatopsis vastitatis sp.
29428970	6	1	theme	Amycolatopsis	1052:1064	arg1	balhymycina					1066:1076	Amycolatopsis balhymycina	1052:1076	Amycolatopsis balhymycina	1052:1076	It was separated readily from the type strain of Amycolatopsis balhymycina, its near phylogenetic neighbour, based on multi-locus sequence data, by low average nucleotide identity (92.9%) and in silico DNA/DNA relatedness values (51.3%) calculated from draft genome assemblies.
29428970	9	2	theme	type	1436:1439	arg1	H5T					1451:1453	H5T	1451:1453	H5T (= NCIMB 14970T = NRRL B-65279T)	1451:1486	The type strain is H5T (= NCIMB 14970T = NRRL B-65279T).
29428970	9	2	theme	type	1436:1439	arg1	strain					1441:1446	The type strain	1432:1446	The type strain	1432:1446	The type strain is H5T (= NCIMB 14970T = NRRL B-65279T).
29428970	7	3	theme	Amycolatopsis	1352:1364	arg1	species					1341:1347	a novel species	1333:1347	a novel species of Amycolatopsis for which the name Amycolatopsis vastitatis sp	1333:1411	Consequently, the strain is considered to represent a novel species of Amycolatopsis for which the name Amycolatopsis vastitatis sp.
29428970	5	4	theme	Amycolatopsis	853:865	arg1	mediterranei					867:878	Amycolatopsis mediterranei	853:878	Amycolatopsis mediterranei	853:878	16S rRNA gene sequence analyses showed that the strain is closely related to the type strain of Amycolatopsis mediterranei but could be distinguished from this and other related Amycolatopsis strains using a broad range of phenotypic properties.
29428970	6	5	theme	nucleotide	1163:1172	arg1	%					1237:1237	51.3%	1233:1237	51.3%	1233:1237	It was separated readily from the type strain of Amycolatopsis balhymycina, its near phylogenetic neighbour, based on multi-locus sequence data, by low average nucleotide identity (92.9%) and in silico DNA/DNA relatedness values (51.3%) calculated from draft genome assemblies.
29428970	6	5	theme	nucleotide	1163:1172	arg1	identity					1174:1181	low average nucleotide identity	1151:1181	low average nucleotide identity (92.9%)	1151:1189	It was separated readily from the type strain of Amycolatopsis balhymycina, its near phylogenetic neighbour, based on multi-locus sequence data, by low average nucleotide identity (92.9%) and in silico DNA/DNA relatedness values (51.3%) calculated from draft genome assemblies.
29428970	6	5	theme	nucleotide	1163:1172	arg1	%					1188:1188	92.9%	1184:1188	92.9%	1184:1188	It was separated readily from the type strain of Amycolatopsis balhymycina, its near phylogenetic neighbour, based on multi-locus sequence data, by low average nucleotide identity (92.9%) and in silico DNA/DNA relatedness values (51.3%) calculated from draft genome assemblies.
29428970	3	6	theme	members	355:361	arg1	typical					344:350	typical	344:350	typical	344:350	The strain, isolate H5T, was shown to have chemical properties typical of members of the genus Amycolatopsis such as meso-diaminopimelic acid as the diamino acid in the cell wall peptidoglycan, arabinose and galactose as diagnostic sugars and MK-9(H4) as the predominant isoprenologue.
29428970	3	7	theme	diagnostic	502:511	arg1	properties					333:342	chemical properties	324:342	chemical properties typical of members of the genus Amycolatopsis such as meso-diaminopimelic acid	324:421	The strain, isolate H5T, was shown to have chemical properties typical of members of the genus Amycolatopsis such as meso-diaminopimelic acid as the diamino acid in the cell wall peptidoglycan, arabinose and galactose as diagnostic sugars and MK-9(H4) as the predominant isoprenologue.
29428970	3	7	theme	diagnostic	502:511	arg1	sugars					513:518	diagnostic sugars	502:518	diagnostic sugars	502:518	The strain, isolate H5T, was shown to have chemical properties typical of members of the genus Amycolatopsis such as meso-diaminopimelic acid as the diamino acid in the cell wall peptidoglycan, arabinose and galactose as diagnostic sugars and MK-9(H4) as the predominant isoprenologue.
29428970	4	8	theme	consistent	617:626	arg1	properties					606:615	cultural and morphological properties	579:615	cultural and morphological properties consistent with its classification in the genus, notably the formation of branching substrate hyphae which fragment into rod-like elements	579:754	It also has cultural and morphological properties consistent with its classification in the genus, notably the formation of branching substrate hyphae which fragment into rod-like elements.
29428970	3	9	theme	chemical	324:331	arg1	properties					333:342	chemical properties	324:342	chemical properties typical of members of the genus Amycolatopsis such as meso-diaminopimelic acid	324:421	The strain, isolate H5T, was shown to have chemical properties typical of members of the genus Amycolatopsis such as meso-diaminopimelic acid as the diamino acid in the cell wall peptidoglycan, arabinose and galactose as diagnostic sugars and MK-9(H4) as the predominant isoprenologue.
29428970	3	9	theme	chemical	324:331	arg1	sugars					513:518	diagnostic sugars	502:518	diagnostic sugars	502:518	The strain, isolate H5T, was shown to have chemical properties typical of members of the genus Amycolatopsis such as meso-diaminopimelic acid as the diamino acid in the cell wall peptidoglycan, arabinose and galactose as diagnostic sugars and MK-9(H4) as the predominant isoprenologue.
29428970	3	9	theme	chemical	324:331	arg1	acid					438:441	the diamino acid	426:441	the diamino acid in the cell wall peptidoglycan	426:472	The strain, isolate H5T, was shown to have chemical properties typical of members of the genus Amycolatopsis such as meso-diaminopimelic acid as the diamino acid in the cell wall peptidoglycan, arabinose and galactose as diagnostic sugars and MK-9(H4) as the predominant isoprenologue.
29428970	7	10	theme	vastitatis	1399:1408	arg1	sp					1410:1411	the name Amycolatopsis vastitatis sp	1376:1411	the name Amycolatopsis vastitatis sp	1376:1411	Consequently, the strain is considered to represent a novel species of Amycolatopsis for which the name Amycolatopsis vastitatis sp.
29428970	1	11	dep	Chile	113:117	arg1	nov.					29:32	nov.	29:32	nov.	29:32	nov., an isolate from a high altitude subsurface soil on Cerro Chajnantor, northern Chile.
29428970	1	11	dep	Chile	113:117	arg1	isolate					38:44	an isolate	35:44	an isolate from a high altitude subsurface soil on Cerro Chajnantor	35:101	nov., an isolate from a high altitude subsurface soil on Cerro Chajnantor, northern Chile.
29428970	3	12	from	arabinose	475:483	arg1	peptidoglycan					460:472	the cell wall peptidoglycan	446:472	the cell wall peptidoglycan	446:472	The strain, isolate H5T, was shown to have chemical properties typical of members of the genus Amycolatopsis such as meso-diaminopimelic acid as the diamino acid in the cell wall peptidoglycan, arabinose and galactose as diagnostic sugars and MK-9(H4) as the predominant isoprenologue.
29428970	2	13	theme	polyphasic	260:269	arg1	approach					271:278	a polyphasic approach	258:278	a polyphasic approach	258:278	The taxonomic position of a novel Amycolatopsis strain isolated from a high altitude Atacama Desert subsurface soil was established using a polyphasic approach.
29428970	6	14	theme	sequence	1133:1140	arg1	data					1142:1145	multi-locus sequence data	1121:1145	multi-locus sequence data	1121:1145	It was separated readily from the type strain of Amycolatopsis balhymycina, its near phylogenetic neighbour, based on multi-locus sequence data, by low average nucleotide identity (92.9%) and in silico DNA/DNA relatedness values (51.3%) calculated from draft genome assemblies.
29428970	3	15	dep	strain	285:290	arg1	isolate					293:299	isolate	293:299	isolate H5T	293:303	The strain, isolate H5T, was shown to have chemical properties typical of members of the genus Amycolatopsis such as meso-diaminopimelic acid as the diamino acid in the cell wall peptidoglycan, arabinose and galactose as diagnostic sugars and MK-9(H4) as the predominant isoprenologue.
29428970	2	16	theme	taxonomic	124:132	arg1	position					134:141	The taxonomic position	120:141	The taxonomic position of a novel Amycolatopsis strain isolated from a high altitude Atacama Desert subsurface soil	120:234	The taxonomic position of a novel Amycolatopsis strain isolated from a high altitude Atacama Desert subsurface soil was established using a polyphasic approach.
29428970	6	17	theme	DNA/DNA	1205:1211	arg1	values					1225:1230	in silico DNA/DNA relatedness values	1195:1230	in silico DNA/DNA relatedness values	1195:1230	It was separated readily from the type strain of Amycolatopsis balhymycina, its near phylogenetic neighbour, based on multi-locus sequence data, by low average nucleotide identity (92.9%) and in silico DNA/DNA relatedness values (51.3%) calculated from draft genome assemblies.
29428970	4	18	theme	branching	691:699	arg1	hyphae					711:716	branching substrate hyphae	691:716	branching substrate hyphae	691:716	It also has cultural and morphological properties consistent with its classification in the genus, notably the formation of branching substrate hyphae which fragment into rod-like elements.
29428970	1	19	theme	high	53:56	arg1	soil					78:81	a high altitude subsurface soil	51:81	a high altitude subsurface soil on Cerro Chajnantor	51:101	nov., an isolate from a high altitude subsurface soil on Cerro Chajnantor, northern Chile.
29428970	3	20	theme	meso-diaminopimelic	398:416	arg1	acid					418:421	meso-diaminopimelic acid	398:421	meso-diaminopimelic acid	398:421	The strain, isolate H5T, was shown to have chemical properties typical of members of the genus Amycolatopsis such as meso-diaminopimelic acid as the diamino acid in the cell wall peptidoglycan, arabinose and galactose as diagnostic sugars and MK-9(H4) as the predominant isoprenologue.
29428970	7	21	theme	name	1380:1383	arg1	sp					1410:1411	the name Amycolatopsis vastitatis sp	1376:1411	the name Amycolatopsis vastitatis sp	1376:1411	Consequently, the strain is considered to represent a novel species of Amycolatopsis for which the name Amycolatopsis vastitatis sp.
29428970	5	22	dep	rRNA	761:764	arg1	analyses					780:787	gene sequence analyses	766:787	16S rRNA gene sequence analyses	757:787	16S rRNA gene sequence analyses showed that the strain is closely related to the type strain of Amycolatopsis mediterranei but could be distinguished from this and other related Amycolatopsis strains using a broad range of phenotypic properties.
29428970	1	23	theme	altitude	58:65	arg1	soil					78:81	a high altitude subsurface soil	51:81	a high altitude subsurface soil on Cerro Chajnantor	51:101	nov., an isolate from a high altitude subsurface soil on Cerro Chajnantor, northern Chile.
29428970	4	24	from	classification	637:650	arg1	genus					659:663	the genus	655:663	the genus	655:663	It also has cultural and morphological properties consistent with its classification in the genus, notably the formation of branching substrate hyphae which fragment into rod-like elements.
29428970	6	25	theme	type	1037:1040	arg1	neighbour					1101:1109	its near phylogenetic neighbour	1079:1109	its near phylogenetic neighbour	1079:1109	It was separated readily from the type strain of Amycolatopsis balhymycina, its near phylogenetic neighbour, based on multi-locus sequence data, by low average nucleotide identity (92.9%) and in silico DNA/DNA relatedness values (51.3%) calculated from draft genome assemblies.
29428970	6	25	theme	type	1037:1040	arg1	strain					1042:1047	the type strain	1033:1047	the type strain of Amycolatopsis balhymycina	1033:1076	It was separated readily from the type strain of Amycolatopsis balhymycina, its near phylogenetic neighbour, based on multi-locus sequence data, by low average nucleotide identity (92.9%) and in silico DNA/DNA relatedness values (51.3%) calculated from draft genome assemblies.
29428970	0	26	theme	vastitatis	14:23	arg1	sp					25:26	Amycolatopsis vastitatis sp	0:26	Amycolatopsis vastitatis sp.	0:27	Amycolatopsis vastitatis sp.
29428970	6	27	theme	multi-locus	1121:1131	arg1	data					1142:1145	multi-locus sequence data	1121:1145	multi-locus sequence data	1121:1145	It was separated readily from the type strain of Amycolatopsis balhymycina, its near phylogenetic neighbour, based on multi-locus sequence data, by low average nucleotide identity (92.9%) and in silico DNA/DNA relatedness values (51.3%) calculated from draft genome assemblies.
29428970	6	28	theme	in	1195:1196	arg1	values					1225:1230	in silico DNA/DNA relatedness values	1195:1230	in silico DNA/DNA relatedness values	1195:1230	It was separated readily from the type strain of Amycolatopsis balhymycina, its near phylogenetic neighbour, based on multi-locus sequence data, by low average nucleotide identity (92.9%) and in silico DNA/DNA relatedness values (51.3%) calculated from draft genome assemblies.
29428970	1	29	theme	subsurface	67:76	arg1	soil					78:81	a high altitude subsurface soil	51:81	a high altitude subsurface soil on Cerro Chajnantor	51:101	nov., an isolate from a high altitude subsurface soil on Cerro Chajnantor, northern Chile.
29428970	6	30	theme	phylogenetic	1088:1099	arg1	strain					1042:1047	the type strain	1033:1047	the type strain of Amycolatopsis balhymycina	1033:1076	It was separated readily from the type strain of Amycolatopsis balhymycina, its near phylogenetic neighbour, based on multi-locus sequence data, by low average nucleotide identity (92.9%) and in silico DNA/DNA relatedness values (51.3%) calculated from draft genome assemblies.
29428970	6	30	theme	phylogenetic	1088:1099	arg1	neighbour					1101:1109	its near phylogenetic neighbour	1079:1109	its near phylogenetic neighbour	1079:1109	It was separated readily from the type strain of Amycolatopsis balhymycina, its near phylogenetic neighbour, based on multi-locus sequence data, by low average nucleotide identity (92.9%) and in silico DNA/DNA relatedness values (51.3%) calculated from draft genome assemblies.
29428970	0	31	theme	Amycolatopsis	0:12	arg1	sp					25:26	Amycolatopsis vastitatis sp	0:26	Amycolatopsis vastitatis sp.	0:27	Amycolatopsis vastitatis sp.
29428970	6	32	theme	average	1155:1161	arg1	%					1237:1237	51.3%	1233:1237	51.3%	1233:1237	It was separated readily from the type strain of Amycolatopsis balhymycina, its near phylogenetic neighbour, based on multi-locus sequence data, by low average nucleotide identity (92.9%) and in silico DNA/DNA relatedness values (51.3%) calculated from draft genome assemblies.
29428970	6	32	theme	average	1155:1161	arg1	identity					1174:1181	low average nucleotide identity	1151:1181	low average nucleotide identity (92.9%)	1151:1189	It was separated readily from the type strain of Amycolatopsis balhymycina, its near phylogenetic neighbour, based on multi-locus sequence data, by low average nucleotide identity (92.9%) and in silico DNA/DNA relatedness values (51.3%) calculated from draft genome assemblies.
29428970	6	32	theme	average	1155:1161	arg1	%					1188:1188	92.9%	1184:1188	92.9%	1184:1188	It was separated readily from the type strain of Amycolatopsis balhymycina, its near phylogenetic neighbour, based on multi-locus sequence data, by low average nucleotide identity (92.9%) and in silico DNA/DNA relatedness values (51.3%) calculated from draft genome assemblies.
29428970	2	33	attach	isolated	175:182	arg1	soil					231:234	a high altitude Atacama Desert subsurface soil	189:234	a high altitude Atacama Desert subsurface soil	189:234	The taxonomic position of a novel Amycolatopsis strain isolated from a high altitude Atacama Desert subsurface soil was established using a polyphasic approach.
29428970	2	33	attach	isolated	175:182	arg2	strain					168:173	a novel Amycolatopsis strain	146:173	a novel Amycolatopsis strain isolated from a high altitude Atacama Desert subsurface soil	146:234	The taxonomic position of a novel Amycolatopsis strain isolated from a high altitude Atacama Desert subsurface soil was established using a polyphasic approach.
29428970	5	34	theme	sequence	771:778	arg1	analyses					780:787	gene sequence analyses	766:787	16S rRNA gene sequence analyses	757:787	16S rRNA gene sequence analyses showed that the strain is closely related to the type strain of Amycolatopsis mediterranei but could be distinguished from this and other related Amycolatopsis strains using a broad range of phenotypic properties.
29428970	4	35	theme	hyphae	711:716	arg1	classification					637:650	its classification	633:650	its classification in the genus	633:663	It also has cultural and morphological properties consistent with its classification in the genus, notably the formation of branching substrate hyphae which fragment into rod-like elements.
29428970	4	35	theme	hyphae	711:716	arg1	formation					678:686	notably the formation	666:686	notably the formation of branching substrate hyphae which fragment into rod-like elements	666:754	It also has cultural and morphological properties consistent with its classification in the genus, notably the formation of branching substrate hyphae which fragment into rod-like elements.
29428970	3	36	theme	diamino	430:436	arg1	properties					333:342	chemical properties	324:342	chemical properties typical of members of the genus Amycolatopsis such as meso-diaminopimelic acid	324:421	The strain, isolate H5T, was shown to have chemical properties typical of members of the genus Amycolatopsis such as meso-diaminopimelic acid as the diamino acid in the cell wall peptidoglycan, arabinose and galactose as diagnostic sugars and MK-9(H4) as the predominant isoprenologue.
29428970	3	36	theme	diamino	430:436	arg1	acid					438:441	the diamino acid	426:441	the diamino acid in the cell wall peptidoglycan	426:472	The strain, isolate H5T, was shown to have chemical properties typical of members of the genus Amycolatopsis such as meso-diaminopimelic acid as the diamino acid in the cell wall peptidoglycan, arabinose and galactose as diagnostic sugars and MK-9(H4) as the predominant isoprenologue.
29428970	3	37	theme	typical	344:350	arg1	properties					333:342	chemical properties	324:342	chemical properties typical of members of the genus Amycolatopsis such as meso-diaminopimelic acid	324:421	The strain, isolate H5T, was shown to have chemical properties typical of members of the genus Amycolatopsis such as meso-diaminopimelic acid as the diamino acid in the cell wall peptidoglycan, arabinose and galactose as diagnostic sugars and MK-9(H4) as the predominant isoprenologue.
29428970	3	37	theme	typical	344:350	arg1	sugars					513:518	diagnostic sugars	502:518	diagnostic sugars	502:518	The strain, isolate H5T, was shown to have chemical properties typical of members of the genus Amycolatopsis such as meso-diaminopimelic acid as the diamino acid in the cell wall peptidoglycan, arabinose and galactose as diagnostic sugars and MK-9(H4) as the predominant isoprenologue.
29428970	3	37	theme	typical	344:350	arg1	acid					438:441	the diamino acid	426:441	the diamino acid in the cell wall peptidoglycan	426:472	The strain, isolate H5T, was shown to have chemical properties typical of members of the genus Amycolatopsis such as meso-diaminopimelic acid as the diamino acid in the cell wall peptidoglycan, arabinose and galactose as diagnostic sugars and MK-9(H4) as the predominant isoprenologue.
29428970	4	38	theme	rod-like	738:745	arg1	elements					747:754	rod-like elements	738:754	rod-like elements	738:754	It also has cultural and morphological properties consistent with its classification in the genus, notably the formation of branching substrate hyphae which fragment into rod-like elements.
29428970	2	39	theme	Amycolatopsis	154:166	arg1	strain					168:173	a novel Amycolatopsis strain	146:173	a novel Amycolatopsis strain isolated from a high altitude Atacama Desert subsurface soil	146:234	The taxonomic position of a novel Amycolatopsis strain isolated from a high altitude Atacama Desert subsurface soil was established using a polyphasic approach.
29428970	4	40	theme	substrate	701:709	arg1	hyphae					711:716	branching substrate hyphae	691:716	branching substrate hyphae	691:716	It also has cultural and morphological properties consistent with its classification in the genus, notably the formation of branching substrate hyphae which fragment into rod-like elements.
29428970	5	41	theme	properties	991:1000	arg1	range					971:975	a broad range	963:975	a broad range of phenotypic properties	963:1000	16S rRNA gene sequence analyses showed that the strain is closely related to the type strain of Amycolatopsis mediterranei but could be distinguished from this and other related Amycolatopsis strains using a broad range of phenotypic properties.
29428970	5	42	theme	related	927:933	arg1	strains					949:955	other related Amycolatopsis strains	921:955	other related Amycolatopsis strains	921:955	16S rRNA gene sequence analyses showed that the strain is closely related to the type strain of Amycolatopsis mediterranei but could be distinguished from this and other related Amycolatopsis strains using a broad range of phenotypic properties.
29428970	3	43	theme	genus	370:374	arg1	Amycolatopsis					376:388	the genus Amycolatopsis	366:388	the genus Amycolatopsis	366:388	The strain, isolate H5T, was shown to have chemical properties typical of members of the genus Amycolatopsis such as meso-diaminopimelic acid as the diamino acid in the cell wall peptidoglycan, arabinose and galactose as diagnostic sugars and MK-9(H4) as the predominant isoprenologue.
29428970	5	44	theme	other	921:925	arg1	strains					949:955	other related Amycolatopsis strains	921:955	other related Amycolatopsis strains	921:955	16S rRNA gene sequence analyses showed that the strain is closely related to the type strain of Amycolatopsis mediterranei but could be distinguished from this and other related Amycolatopsis strains using a broad range of phenotypic properties.
29428970	6	45	theme	genome	1262:1267	arg1	assemblies					1269:1278	draft genome assemblies	1256:1278	draft genome assemblies	1256:1278	It was separated readily from the type strain of Amycolatopsis balhymycina, its near phylogenetic neighbour, based on multi-locus sequence data, by low average nucleotide identity (92.9%) and in silico DNA/DNA relatedness values (51.3%) calculated from draft genome assemblies.
29428970	2	46	theme	novel	148:152	arg1	strain					168:173	a novel Amycolatopsis strain	146:173	a novel Amycolatopsis strain isolated from a high altitude Atacama Desert subsurface soil	146:234	The taxonomic position of a novel Amycolatopsis strain isolated from a high altitude Atacama Desert subsurface soil was established using a polyphasic approach.
29428970	2	47	theme	Desert	213:218	arg1	soil					231:234	a high altitude Atacama Desert subsurface soil	189:234	a high altitude Atacama Desert subsurface soil	189:234	The taxonomic position of a novel Amycolatopsis strain isolated from a high altitude Atacama Desert subsurface soil was established using a polyphasic approach.
29428970	4	48	theme	morphological	592:604	arg1	properties					606:615	cultural and morphological properties	579:615	cultural and morphological properties consistent with its classification in the genus, notably the formation of branching substrate hyphae which fragment into rod-like elements	579:754	It also has cultural and morphological properties consistent with its classification in the genus, notably the formation of branching substrate hyphae which fragment into rod-like elements.
29428970	3	49	theme	Amycolatopsis	376:388	arg1	acid					418:421	meso-diaminopimelic acid	398:421	meso-diaminopimelic acid	398:421	The strain, isolate H5T, was shown to have chemical properties typical of members of the genus Amycolatopsis such as meso-diaminopimelic acid as the diamino acid in the cell wall peptidoglycan, arabinose and galactose as diagnostic sugars and MK-9(H4) as the predominant isoprenologue.
29428970	3	49	theme	Amycolatopsis	376:388	arg1	members					355:361	members	355:361	members of the genus Amycolatopsis such as meso-diaminopimelic acid	355:421	The strain, isolate H5T, was shown to have chemical properties typical of members of the genus Amycolatopsis such as meso-diaminopimelic acid as the diamino acid in the cell wall peptidoglycan, arabinose and galactose as diagnostic sugars and MK-9(H4) as the predominant isoprenologue.
29428970	6	50	theme	draft	1256:1260	arg1	assemblies					1269:1278	draft genome assemblies	1256:1278	draft genome assemblies	1256:1278	It was separated readily from the type strain of Amycolatopsis balhymycina, its near phylogenetic neighbour, based on multi-locus sequence data, by low average nucleotide identity (92.9%) and in silico DNA/DNA relatedness values (51.3%) calculated from draft genome assemblies.
29428970	4	51	contain	has	575:577	arg1	It					567:568	It	567:568	It	567:568	It also has cultural and morphological properties consistent with its classification in the genus, notably the formation of branching substrate hyphae which fragment into rod-like elements.
29428970	4	51	contain	has	575:577	arg2	properties					606:615	cultural and morphological properties	579:615	cultural and morphological properties consistent with its classification in the genus, notably the formation of branching substrate hyphae which fragment into rod-like elements	579:754	It also has cultural and morphological properties consistent with its classification in the genus, notably the formation of branching substrate hyphae which fragment into rod-like elements.
29428970	2	52	theme	Atacama	205:211	arg1	soil					231:234	a high altitude Atacama Desert subsurface soil	189:234	a high altitude Atacama Desert subsurface soil	189:234	The taxonomic position of a novel Amycolatopsis strain isolated from a high altitude Atacama Desert subsurface soil was established using a polyphasic approach.
29428970	4	53	with	consistent	617:626	arg1	classification					637:650	its classification	633:650	its classification in the genus	633:663	It also has cultural and morphological properties consistent with its classification in the genus, notably the formation of branching substrate hyphae which fragment into rod-like elements.
29428970	4	53	with	consistent	617:626	arg1	formation					678:686	notably the formation	666:686	notably the formation of branching substrate hyphae which fragment into rod-like elements	666:754	It also has cultural and morphological properties consistent with its classification in the genus, notably the formation of branching substrate hyphae which fragment into rod-like elements.
29428970	2	54	theme	altitude	196:203	arg1	soil					231:234	a high altitude Atacama Desert subsurface soil	189:234	a high altitude Atacama Desert subsurface soil	189:234	The taxonomic position of a novel Amycolatopsis strain isolated from a high altitude Atacama Desert subsurface soil was established using a polyphasic approach.
29428970	9	55	theme	= NCIMB	1456:1462	arg1	H5T					1451:1453	H5T	1451:1453	H5T (= NCIMB 14970T = NRRL B-65279T)	1451:1486	The type strain is H5T (= NCIMB 14970T = NRRL B-65279T).
29428970	9	55	theme	= NCIMB	1456:1462	arg1	B-65279T					1478:1485	= NCIMB 14970T = NRRL B-65279T	1456:1485	= NCIMB 14970T = NRRL B-65279T	1456:1485	The type strain is H5T (= NCIMB 14970T = NRRL B-65279T).
29428970	3	56	theme	cell	450:453	arg1	peptidoglycan					460:472	the cell wall peptidoglycan	446:472	the cell wall peptidoglycan	446:472	The strain, isolate H5T, was shown to have chemical properties typical of members of the genus Amycolatopsis such as meso-diaminopimelic acid as the diamino acid in the cell wall peptidoglycan, arabinose and galactose as diagnostic sugars and MK-9(H4) as the predominant isoprenologue.
29428970	6	57	theme	relatedness	1213:1223	arg1	values					1225:1230	in silico DNA/DNA relatedness values	1195:1230	in silico DNA/DNA relatedness values	1195:1230	It was separated readily from the type strain of Amycolatopsis balhymycina, its near phylogenetic neighbour, based on multi-locus sequence data, by low average nucleotide identity (92.9%) and in silico DNA/DNA relatedness values (51.3%) calculated from draft genome assemblies.
29428970	9	58	theme	14970T = NRRL	1464:1476	arg1	H5T					1451:1453	H5T	1451:1453	H5T (= NCIMB 14970T = NRRL B-65279T)	1451:1486	The type strain is H5T (= NCIMB 14970T = NRRL B-65279T).
29428970	9	58	theme	14970T = NRRL	1464:1476	arg1	B-65279T					1478:1485	= NCIMB 14970T = NRRL B-65279T	1456:1485	= NCIMB 14970T = NRRL B-65279T	1456:1485	The type strain is H5T (= NCIMB 14970T = NRRL B-65279T).
29428970	2	59	theme	high	191:194	arg1	soil					231:234	a high altitude Atacama Desert subsurface soil	189:234	a high altitude Atacama Desert subsurface soil	189:234	The taxonomic position of a novel Amycolatopsis strain isolated from a high altitude Atacama Desert subsurface soil was established using a polyphasic approach.
29428970	3	60	theme	wall	455:458	arg1	peptidoglycan					460:472	the cell wall peptidoglycan	446:472	the cell wall peptidoglycan	446:472	The strain, isolate H5T, was shown to have chemical properties typical of members of the genus Amycolatopsis such as meso-diaminopimelic acid as the diamino acid in the cell wall peptidoglycan, arabinose and galactose as diagnostic sugars and MK-9(H4) as the predominant isoprenologue.
29428970	6	61	theme	low	1151:1153	arg1	%					1237:1237	51.3%	1233:1237	51.3%	1233:1237	It was separated readily from the type strain of Amycolatopsis balhymycina, its near phylogenetic neighbour, based on multi-locus sequence data, by low average nucleotide identity (92.9%) and in silico DNA/DNA relatedness values (51.3%) calculated from draft genome assemblies.
29428970	6	61	theme	low	1151:1153	arg1	identity					1174:1181	low average nucleotide identity	1151:1181	low average nucleotide identity (92.9%)	1151:1189	It was separated readily from the type strain of Amycolatopsis balhymycina, its near phylogenetic neighbour, based on multi-locus sequence data, by low average nucleotide identity (92.9%) and in silico DNA/DNA relatedness values (51.3%) calculated from draft genome assemblies.
29428970	6	61	theme	low	1151:1153	arg1	%					1188:1188	92.9%	1184:1188	92.9%	1184:1188	It was separated readily from the type strain of Amycolatopsis balhymycina, its near phylogenetic neighbour, based on multi-locus sequence data, by low average nucleotide identity (92.9%) and in silico DNA/DNA relatedness values (51.3%) calculated from draft genome assemblies.
29428970	5	62	theme	phenotypic	980:989	arg1	properties					991:1000	phenotypic properties	980:1000	phenotypic properties	980:1000	16S rRNA gene sequence analyses showed that the strain is closely related to the type strain of Amycolatopsis mediterranei but could be distinguished from this and other related Amycolatopsis strains using a broad range of phenotypic properties.
29428970	3	63	theme	predominant	540:550	arg1	isoprenologue					552:564	the predominant isoprenologue	536:564	the predominant isoprenologue	536:564	The strain, isolate H5T, was shown to have chemical properties typical of members of the genus Amycolatopsis such as meso-diaminopimelic acid as the diamino acid in the cell wall peptidoglycan, arabinose and galactose as diagnostic sugars and MK-9(H4) as the predominant isoprenologue.
29428970	3	64	contain	have	319:322	arg2	acid					438:441	the diamino acid	426:441	the diamino acid in the cell wall peptidoglycan	426:472	The strain, isolate H5T, was shown to have chemical properties typical of members of the genus Amycolatopsis such as meso-diaminopimelic acid as the diamino acid in the cell wall peptidoglycan, arabinose and galactose as diagnostic sugars and MK-9(H4) as the predominant isoprenologue.
29428970	3	64	contain	have	319:322	arg2	properties					333:342	chemical properties	324:342	chemical properties typical of members of the genus Amycolatopsis such as meso-diaminopimelic acid	324:421	The strain, isolate H5T, was shown to have chemical properties typical of members of the genus Amycolatopsis such as meso-diaminopimelic acid as the diamino acid in the cell wall peptidoglycan, arabinose and galactose as diagnostic sugars and MK-9(H4) as the predominant isoprenologue.
29428970	3	64	contain	have	319:322	arg1	strain					285:290	The strain	281:290	The strain	281:290	The strain, isolate H5T, was shown to have chemical properties typical of members of the genus Amycolatopsis such as meso-diaminopimelic acid as the diamino acid in the cell wall peptidoglycan, arabinose and galactose as diagnostic sugars and MK-9(H4) as the predominant isoprenologue.
29428970	3	64	contain	have	319:322	arg2	sugars					513:518	diagnostic sugars	502:518	diagnostic sugars	502:518	The strain, isolate H5T, was shown to have chemical properties typical of members of the genus Amycolatopsis such as meso-diaminopimelic acid as the diamino acid in the cell wall peptidoglycan, arabinose and galactose as diagnostic sugars and MK-9(H4) as the predominant isoprenologue.
29428970	1	65	theme	northern	104:111	arg1	Chile					113:117	northern Chile	104:117	northern Chile	104:117	nov., an isolate from a high altitude subsurface soil on Cerro Chajnantor, northern Chile.
29428970	7	66	theme	novel	1335:1339	arg1	species					1341:1347	a novel species	1333:1347	a novel species of Amycolatopsis for which the name Amycolatopsis vastitatis sp	1333:1411	Consequently, the strain is considered to represent a novel species of Amycolatopsis for which the name Amycolatopsis vastitatis sp.
29428970	3	67	from	acid	438:441	arg1	peptidoglycan					460:472	the cell wall peptidoglycan	446:472	the cell wall peptidoglycan	446:472	The strain, isolate H5T, was shown to have chemical properties typical of members of the genus Amycolatopsis such as meso-diaminopimelic acid as the diamino acid in the cell wall peptidoglycan, arabinose and galactose as diagnostic sugars and MK-9(H4) as the predominant isoprenologue.
29428970	5	68	theme	mediterranei	867:878	arg1	strain					843:848	the type strain	834:848	the type strain of Amycolatopsis mediterranei	834:878	16S rRNA gene sequence analyses showed that the strain is closely related to the type strain of Amycolatopsis mediterranei but could be distinguished from this and other related Amycolatopsis strains using a broad range of phenotypic properties.
29428970	5	69	theme	Amycolatopsis	935:947	arg1	strains					949:955	other related Amycolatopsis strains	921:955	other related Amycolatopsis strains	921:955	16S rRNA gene sequence analyses showed that the strain is closely related to the type strain of Amycolatopsis mediterranei but could be distinguished from this and other related Amycolatopsis strains using a broad range of phenotypic properties.
29428970	6	70	theme	balhymycina	1066:1076	arg1	neighbour					1101:1109	its near phylogenetic neighbour	1079:1109	its near phylogenetic neighbour	1079:1109	It was separated readily from the type strain of Amycolatopsis balhymycina, its near phylogenetic neighbour, based on multi-locus sequence data, by low average nucleotide identity (92.9%) and in silico DNA/DNA relatedness values (51.3%) calculated from draft genome assemblies.
29428970	6	70	theme	balhymycina	1066:1076	arg1	strain					1042:1047	the type strain	1033:1047	the type strain of Amycolatopsis balhymycina	1033:1076	It was separated readily from the type strain of Amycolatopsis balhymycina, its near phylogenetic neighbour, based on multi-locus sequence data, by low average nucleotide identity (92.9%) and in silico DNA/DNA relatedness values (51.3%) calculated from draft genome assemblies.
29428970	6	71	theme	near	1083:1086	arg1	strain					1042:1047	the type strain	1033:1047	the type strain of Amycolatopsis balhymycina	1033:1076	It was separated readily from the type strain of Amycolatopsis balhymycina, its near phylogenetic neighbour, based on multi-locus sequence data, by low average nucleotide identity (92.9%) and in silico DNA/DNA relatedness values (51.3%) calculated from draft genome assemblies.
29428970	6	71	theme	near	1083:1086	arg1	neighbour					1101:1109	its near phylogenetic neighbour	1079:1109	its near phylogenetic neighbour	1079:1109	It was separated readily from the type strain of Amycolatopsis balhymycina, its near phylogenetic neighbour, based on multi-locus sequence data, by low average nucleotide identity (92.9%) and in silico DNA/DNA relatedness values (51.3%) calculated from draft genome assemblies.
29428970	6	72	dep	in	1195:1196	arg1	silico					1198:1203	silico	1198:1203	silico	1198:1203	It was separated readily from the type strain of Amycolatopsis balhymycina, its near phylogenetic neighbour, based on multi-locus sequence data, by low average nucleotide identity (92.9%) and in silico DNA/DNA relatedness values (51.3%) calculated from draft genome assemblies.
29428970	2	73	theme	strain	168:173	arg1	position					134:141	The taxonomic position	120:141	The taxonomic position of a novel Amycolatopsis strain isolated from a high altitude Atacama Desert subsurface soil	120:234	The taxonomic position of a novel Amycolatopsis strain isolated from a high altitude Atacama Desert subsurface soil was established using a polyphasic approach.
29428970	5	74	theme	broad	965:969	arg1	range					971:975	a broad range	963:975	a broad range of phenotypic properties	963:1000	16S rRNA gene sequence analyses showed that the strain is closely related to the type strain of Amycolatopsis mediterranei but could be distinguished from this and other related Amycolatopsis strains using a broad range of phenotypic properties.
29428970	4	75	theme	cultural	579:586	arg1	properties					606:615	cultural and morphological properties	579:615	cultural and morphological properties consistent with its classification in the genus, notably the formation of branching substrate hyphae which fragment into rod-like elements	579:754	It also has cultural and morphological properties consistent with its classification in the genus, notably the formation of branching substrate hyphae which fragment into rod-like elements.
29428970	5	76	theme	type	838:841	arg1	strain					843:848	the type strain	834:848	the type strain of Amycolatopsis mediterranei	834:878	16S rRNA gene sequence analyses showed that the strain is closely related to the type strain of Amycolatopsis mediterranei but could be distinguished from this and other related Amycolatopsis strains using a broad range of phenotypic properties.
29428970	1	77	from	soil	78:81	arg1	Chajnantor					92:101	Chajnantor	92:101	Chajnantor	92:101	nov., an isolate from a high altitude subsurface soil on Cerro Chajnantor, northern Chile.
29428970	1	77	from	soil	78:81	arg1	nov.					29:32	nov.	29:32	nov.	29:32	nov., an isolate from a high altitude subsurface soil on Cerro Chajnantor, northern Chile.
29428970	1	77	from	soil	78:81	arg1	isolate					38:44	an isolate	35:44	an isolate from a high altitude subsurface soil on Cerro Chajnantor	35:101	nov., an isolate from a high altitude subsurface soil on Cerro Chajnantor, northern Chile.
29428970	5	78	theme	16S	757:759	arg1	rRNA					761:764	16S rRNA gene sequence analyses	757:787	16S rRNA gene sequence analyses	757:787	16S rRNA gene sequence analyses showed that the strain is closely related to the type strain of Amycolatopsis mediterranei but could be distinguished from this and other related Amycolatopsis strains using a broad range of phenotypic properties.
29428970	2	79	theme	subsurface	220:229	arg1	soil					231:234	a high altitude Atacama Desert subsurface soil	189:234	a high altitude Atacama Desert subsurface soil	189:234	The taxonomic position of a novel Amycolatopsis strain isolated from a high altitude Atacama Desert subsurface soil was established using a polyphasic approach.
29428970	5	80	theme	gene	766:769	arg1	analyses					780:787	gene sequence analyses	766:787	16S rRNA gene sequence analyses	757:787	16S rRNA gene sequence analyses showed that the strain is closely related to the type strain of Amycolatopsis mediterranei but could be distinguished from this and other related Amycolatopsis strains using a broad range of phenotypic properties.
29428970	3	81	from	galactose	489:497	arg1	peptidoglycan					460:472	the cell wall peptidoglycan	446:472	the cell wall peptidoglycan	446:472	The strain, isolate H5T, was shown to have chemical properties typical of members of the genus Amycolatopsis such as meso-diaminopimelic acid as the diamino acid in the cell wall peptidoglycan, arabinose and galactose as diagnostic sugars and MK-9(H4) as the predominant isoprenologue.
31127044	5	0	theme	MZB1-deficient	705:718	arg1	mice					720:723	MZB1-deficient mice	705:723	MZB1-deficient mice	705:723	MZB1-deficient mice are impaired in secreting large amounts of IgA into the gut in response to acute inflammation and develop severe colitis.
31127044	6	1	theme	gut	962:964	arg1	composition					977:987	the gut microbiota composition	958:987	the gut microbiota composition	958:987	Oral administration of a monoclonal IgA significantly ameliorated the colitis, accompanied by normalization of the gut microbiota composition.
31127044	3	2	theme	cell-specific	423:435	arg1	protein					437:443	B-1 cell-specific protein	419:443	B-1 cell-specific protein (MZB1)	419:450	Here, we demonstrate that the marginal zone B and B-1 cell-specific protein (MZB1) interacts with IgA through the α-heavy-chain tailpiece dependent on the penultimate cysteine residue and prevents the intracellular degradation of α-light-chain complexes.
31127044	3	2	theme	cell-specific	423:435	arg1	MZB1					446:449	MZB1	446:449	MZB1	446:449	Here, we demonstrate that the marginal zone B and B-1 cell-specific protein (MZB1) interacts with IgA through the α-heavy-chain tailpiece dependent on the penultimate cysteine residue and prevents the intracellular degradation of α-light-chain complexes.
31127044	7	3	theme	IgA	1166:1168	arg1	quality					1141:1147	quality	1141:1147	quality	1141:1147	The present study identifies a molecular chaperone that promotes J-chain binding to IgA and reveals an important mechanism that controls the quantity, quality, and function of IgA.
31127044	7	3	theme	IgA	1166:1168	arg1	function					1154:1161	function	1154:1161	function	1154:1161	The present study identifies a molecular chaperone that promotes J-chain binding to IgA and reveals an important mechanism that controls the quantity, quality, and function of IgA.
31127044	7	3	theme	IgA	1166:1168	arg1	quantity					1131:1138	quantity	1131:1138	quantity	1131:1138	The present study identifies a molecular chaperone that promotes J-chain binding to IgA and reveals an important mechanism that controls the quantity, quality, and function of IgA.
31127044	6	4	theme	Oral	847:850	arg1	administration					852:865	Oral administration	847:865	Oral administration of a monoclonal IgA	847:885	Oral administration of a monoclonal IgA significantly ameliorated the colitis, accompanied by normalization of the gut microbiota composition.
31127044	5	5	theme	severe	831:836	arg1	colitis					838:844	severe colitis	831:844	severe colitis	831:844	MZB1-deficient mice are impaired in secreting large amounts of IgA into the gut in response to acute inflammation and develop severe colitis.
31127044	1	6	theme	produced	146:153	arg1	IgA					119:121	IgA	119:121	IgA	119:121	IgA is the most abundantly produced antibody in the body and plays a crucial role in gut homeostasis and mucosal immunity.
31127044	1	6	theme	produced	146:153	arg1	antibody					155:162	the most abundantly produced antibody	126:162	the most abundantly produced antibody	126:162	IgA is the most abundantly produced antibody in the body and plays a crucial role in gut homeostasis and mucosal immunity.
31127044	1	7	theme	mucosal	224:230	arg1	immunity					232:239	mucosal immunity	224:239	mucosal immunity	224:239	IgA is the most abundantly produced antibody in the body and plays a crucial role in gut homeostasis and mucosal immunity.
31127044	7	8	dep	quantity	1131:1138	arg1	the					1127:1129	the	1127:1129	the	1127:1129	The present study identifies a molecular chaperone that promotes J-chain binding to IgA and reveals an important mechanism that controls the quantity, quality, and function of IgA.
31127044	3	9	dep	zone	408:411	arg1	protein					437:443	B-1 cell-specific protein	419:443	B-1 cell-specific protein (MZB1)	419:450	Here, we demonstrate that the marginal zone B and B-1 cell-specific protein (MZB1) interacts with IgA through the α-heavy-chain tailpiece dependent on the penultimate cysteine residue and prevents the intracellular degradation of α-light-chain complexes.
31127044	3	9	dep	zone	408:411	arg1	B					413:413	B	413:413	B	413:413	Here, we demonstrate that the marginal zone B and B-1 cell-specific protein (MZB1) interacts with IgA through the α-heavy-chain tailpiece dependent on the penultimate cysteine residue and prevents the intracellular degradation of α-light-chain complexes.
31127044	3	9	dep	zone	408:411	arg1	MZB1					446:449	MZB1	446:449	MZB1	446:449	Here, we demonstrate that the marginal zone B and B-1 cell-specific protein (MZB1) interacts with IgA through the α-heavy-chain tailpiece dependent on the penultimate cysteine residue and prevents the intracellular degradation of α-light-chain complexes.
31127044	4	10	theme	IgA	700:702	arg1	secretion					679:687	the secretion	675:687	the secretion of dimeric IgA	675:702	Moreover, MZB1 promotes J-chain binding to IgA and the secretion of dimeric IgA.
31127044	4	10	theme	IgA	700:702	arg1	binding					656:662	J-chain binding	648:662	J-chain binding to IgA	648:669	Moreover, MZB1 promotes J-chain binding to IgA and the secretion of dimeric IgA.
31127044	4	11	theme	J-chain	648:654	arg1	binding					656:662	J-chain binding	648:662	J-chain binding to IgA	648:669	Moreover, MZB1 promotes J-chain binding to IgA and the secretion of dimeric IgA.
31127044	0	12	theme	gut	101:103	arg1	inflammation					105:116	gut inflammation	101:116	gut inflammation	101:116	MZB1 promotes the secretion of J-chain-containing dimeric IgA and is critical for the suppression of gut inflammation.
31127044	7	13	theme	J-chain	1055:1061	arg1	binding					1063:1069	J-chain binding	1055:1069	J-chain binding to IgA	1055:1076	The present study identifies a molecular chaperone that promotes J-chain binding to IgA and reveals an important mechanism that controls the quantity, quality, and function of IgA.
31127044	2	14	theme	IgA	338:340	arg1	transport					342:350	IgA transport	338:350	IgA transport into the mucosa	338:366	IgA forms a dimer that covalently associates with the joining (J) chain, which is essential for IgA transport into the mucosa.
31127044	7	15	theme	important	1093:1101	arg1	mechanism					1103:1111	an important mechanism	1090:1111	an important mechanism that controls the quantity, quality, and function of IgA	1090:1168	The present study identifies a molecular chaperone that promotes J-chain binding to IgA and reveals an important mechanism that controls the quantity, quality, and function of IgA.
31127044	5	16	theme	large	751:755	arg1	amounts					757:763	large amounts	751:763	large amounts of IgA	751:770	MZB1-deficient mice are impaired in secreting large amounts of IgA into the gut in response to acute inflammation and develop severe colitis.
31127044	5	16	theme	large	751:755	arg1	IgA					768:770	IgA	768:770	IgA	768:770	MZB1-deficient mice are impaired in secreting large amounts of IgA into the gut in response to acute inflammation and develop severe colitis.
31127044	3	17	theme	α-heavy-chain	483:495	arg1	tailpiece					497:505	the α-heavy-chain tailpiece	479:505	the α-heavy-chain tailpiece dependent on the penultimate cysteine residue	479:551	Here, we demonstrate that the marginal zone B and B-1 cell-specific protein (MZB1) interacts with IgA through the α-heavy-chain tailpiece dependent on the penultimate cysteine residue and prevents the intracellular degradation of α-light-chain complexes.
31127044	5	18	theme	acute	800:804	arg1	inflammation					806:817	acute inflammation	800:817	acute inflammation	800:817	MZB1-deficient mice are impaired in secreting large amounts of IgA into the gut in response to acute inflammation and develop severe colitis.
31127044	7	19	theme	molecular	1021:1029	arg1	chaperone					1031:1039	a molecular chaperone	1019:1039	a molecular chaperone that promotes J-chain binding to IgA and reveals an important mechanism that controls the quantity, quality, and function of IgA	1019:1168	The present study identifies a molecular chaperone that promotes J-chain binding to IgA and reveals an important mechanism that controls the quantity, quality, and function of IgA.
31127044	4	20	theme	dimeric	692:698	arg1	IgA					700:702	dimeric IgA	692:702	dimeric IgA	692:702	Moreover, MZB1 promotes J-chain binding to IgA and the secretion of dimeric IgA.
31127044	5	21	theme	IgA	768:770	arg1	amounts					757:763	large amounts	751:763	large amounts of IgA	751:770	MZB1-deficient mice are impaired in secreting large amounts of IgA into the gut in response to acute inflammation and develop severe colitis.
31127044	5	21	theme	IgA	768:770	arg1	IgA					768:770	IgA	768:770	IgA	768:770	MZB1-deficient mice are impaired in secreting large amounts of IgA into the gut in response to acute inflammation and develop severe colitis.
31127044	0	22	theme	inflammation	105:116	arg1	suppression					86:96	the suppression	82:96	the suppression of gut inflammation	82:116	MZB1 promotes the secretion of J-chain-containing dimeric IgA and is critical for the suppression of gut inflammation.
31127044	3	23	theme	marginal	399:406	arg1	zone					408:411	the marginal zone B and B-1 cell-specific protein (MZB1)	395:450	the marginal zone B and B-1 cell-specific protein (MZB1)	395:450	Here, we demonstrate that the marginal zone B and B-1 cell-specific protein (MZB1) interacts with IgA through the α-heavy-chain tailpiece dependent on the penultimate cysteine residue and prevents the intracellular degradation of α-light-chain complexes.
31127044	0	24	theme	J-chain-containing	31:48	arg1	IgA					58:60	J-chain-containing dimeric IgA	31:60	J-chain-containing dimeric IgA	31:60	MZB1 promotes the secretion of J-chain-containing dimeric IgA and is critical for the suppression of gut inflammation.
31127044	3	25	theme	penultimate	524:534	arg1	residue					545:551	the penultimate cysteine residue	520:551	the penultimate cysteine residue	520:551	Here, we demonstrate that the marginal zone B and B-1 cell-specific protein (MZB1) interacts with IgA through the α-heavy-chain tailpiece dependent on the penultimate cysteine residue and prevents the intracellular degradation of α-light-chain complexes.
31127044	1	26	theme	crucial	188:194	arg1	role					196:199	a crucial role	186:199	a crucial role	186:199	IgA is the most abundantly produced antibody in the body and plays a crucial role in gut homeostasis and mucosal immunity.
31127044	0	27	theme	IgA	58:60	arg1	secretion					18:26	the secretion	14:26	the secretion of J-chain-containing dimeric IgA	14:60	MZB1 promotes the secretion of J-chain-containing dimeric IgA and is critical for the suppression of gut inflammation.
31127044	6	28	theme	IgA	883:885	arg1	administration					852:865	Oral administration	847:865	Oral administration of a monoclonal IgA	847:885	Oral administration of a monoclonal IgA significantly ameliorated the colitis, accompanied by normalization of the gut microbiota composition.
31127044	7	29	theme	present	994:1000	arg1	study					1002:1006	The present study	990:1006	The present study	990:1006	The present study identifies a molecular chaperone that promotes J-chain binding to IgA and reveals an important mechanism that controls the quantity, quality, and function of IgA.
31127044	3	30	theme	intracellular	570:582	arg1	degradation					584:594	the intracellular degradation	566:594	the intracellular degradation of α-light-chain complexes	566:621	Here, we demonstrate that the marginal zone B and B-1 cell-specific protein (MZB1) interacts with IgA through the α-heavy-chain tailpiece dependent on the penultimate cysteine residue and prevents the intracellular degradation of α-light-chain complexes.
31127044	3	31	theme	dependent	507:515	arg1	tailpiece					497:505	the α-heavy-chain tailpiece	479:505	the α-heavy-chain tailpiece dependent on the penultimate cysteine residue	479:551	Here, we demonstrate that the marginal zone B and B-1 cell-specific protein (MZB1) interacts with IgA through the α-heavy-chain tailpiece dependent on the penultimate cysteine residue and prevents the intracellular degradation of α-light-chain complexes.
31127044	2	32	theme	J	305:305	arg1	chain					308:312	the joining (J) chain	292:312	the joining (J) chain	292:312	IgA forms a dimer that covalently associates with the joining (J) chain, which is essential for IgA transport into the mucosa.
31127044	2	32	theme	J	305:305	arg1	essential					324:332	essential	324:332	essential	324:332	IgA forms a dimer that covalently associates with the joining (J) chain, which is essential for IgA transport into the mucosa.
31127044	0	33	theme	dimeric	50:56	arg1	IgA					58:60	J-chain-containing dimeric IgA	31:60	J-chain-containing dimeric IgA	31:60	MZB1 promotes the secretion of J-chain-containing dimeric IgA and is critical for the suppression of gut inflammation.
31127044	6	34	theme	monoclonal	872:881	arg1	IgA					883:885	a monoclonal IgA	870:885	a monoclonal IgA	870:885	Oral administration of a monoclonal IgA significantly ameliorated the colitis, accompanied by normalization of the gut microbiota composition.
31127044	3	35	theme	B-1	419:421	arg1	protein					437:443	B-1 cell-specific protein	419:443	B-1 cell-specific protein (MZB1)	419:450	Here, we demonstrate that the marginal zone B and B-1 cell-specific protein (MZB1) interacts with IgA through the α-heavy-chain tailpiece dependent on the penultimate cysteine residue and prevents the intracellular degradation of α-light-chain complexes.
31127044	3	35	theme	B-1	419:421	arg1	MZB1					446:449	MZB1	446:449	MZB1	446:449	Here, we demonstrate that the marginal zone B and B-1 cell-specific protein (MZB1) interacts with IgA through the α-heavy-chain tailpiece dependent on the penultimate cysteine residue and prevents the intracellular degradation of α-light-chain complexes.
31127044	5	36	dep	inflammation	806:817	arg1	response					788:795	response	788:795	response	788:795	MZB1-deficient mice are impaired in secreting large amounts of IgA into the gut in response to acute inflammation and develop severe colitis.
31127044	2	37	theme	joining	296:302	arg1	chain					308:312	the joining (J) chain	292:312	the joining (J) chain	292:312	IgA forms a dimer that covalently associates with the joining (J) chain, which is essential for IgA transport into the mucosa.
31127044	2	37	theme	joining	296:302	arg1	essential					324:332	essential	324:332	essential	324:332	IgA forms a dimer that covalently associates with the joining (J) chain, which is essential for IgA transport into the mucosa.
31127044	6	38	theme	composition	977:987	arg1	normalization					941:953	normalization	941:953	normalization of the gut microbiota composition	941:987	Oral administration of a monoclonal IgA significantly ameliorated the colitis, accompanied by normalization of the gut microbiota composition.
31127044	3	39	theme	α-light-chain	599:611	arg1	complexes					613:621	α-light-chain complexes	599:621	α-light-chain complexes	599:621	Here, we demonstrate that the marginal zone B and B-1 cell-specific protein (MZB1) interacts with IgA through the α-heavy-chain tailpiece dependent on the penultimate cysteine residue and prevents the intracellular degradation of α-light-chain complexes.
31127044	6	40	theme	microbiota	966:975	arg1	composition					977:987	the gut microbiota composition	958:987	the gut microbiota composition	958:987	Oral administration of a monoclonal IgA significantly ameliorated the colitis, accompanied by normalization of the gut microbiota composition.
31127044	3	41	theme	complexes	613:621	arg1	degradation					584:594	the intracellular degradation	566:594	the intracellular degradation of α-light-chain complexes	566:621	Here, we demonstrate that the marginal zone B and B-1 cell-specific protein (MZB1) interacts with IgA through the α-heavy-chain tailpiece dependent on the penultimate cysteine residue and prevents the intracellular degradation of α-light-chain complexes.
31127044	3	42	theme	cysteine	536:543	arg1	residue					545:551	the penultimate cysteine residue	520:551	the penultimate cysteine residue	520:551	Here, we demonstrate that the marginal zone B and B-1 cell-specific protein (MZB1) interacts with IgA through the α-heavy-chain tailpiece dependent on the penultimate cysteine residue and prevents the intracellular degradation of α-light-chain complexes.
31127044	1	43	theme	gut	204:206	arg1	homeostasis					208:218	gut homeostasis	204:218	gut homeostasis	204:218	IgA is the most abundantly produced antibody in the body and plays a crucial role in gut homeostasis and mucosal immunity.
31127044	1	44	from	antibody	155:162	arg1	body					171:174	the body	167:174	the body	167:174	IgA is the most abundantly produced antibody in the body and plays a crucial role in gut homeostasis and mucosal immunity.
30010169	9	0	theme	gut	1645:1647	arg1	microbiota					1649:1658	the disturbed gut microbiota	1631:1658	the disturbed gut microbiota	1631:1658	These findings suggest that LC27, LC67, and LM can alleviate alcoholic steatosis by inhibiting LPS-mediated NF-κB activation through restoration of the disturbed gut microbiota.
30010169	5	1	theme	actin	843:847	arg1	expression					849:858	α-smooth muscle actin expression	827:858	α-smooth muscle actin expression in the liver	827:871	These also suppressed ethanol-induced NF-κB activation and α-smooth muscle actin expression in the liver and increased ethanol-suppressed AMPK activation.
30010169	4	2	theme	TG	726:727	arg1	levels					737:742	ethanol-induced ALT, AST, TG, and TC levels	700:742	ethanol-induced ALT, AST, TG, and TC levels in the blood and liver	700:765	Oral administration of LC27, LC67, or LM in mice reduced ethanol-induced ALT, AST, TG, and TC levels in the blood and liver.
30010169	7	3	theme	blood	1310:1314	arg1	levels					1335:1340	blood lipopolysaccharide levels	1310:1340	blood lipopolysaccharide levels	1310:1340	Moreover, treatment with LC27, LC67, or LM restored the ethanol-disturbed gut microbiota composition, such as the increased population of Proteobacteria, and inhibited fecal and blood lipopolysaccharide levels.
30010169	9	4	theme	microbiota	1649:1658	arg1	restoration					1616:1626	restoration	1616:1626	restoration of the disturbed gut microbiota	1616:1658	These findings suggest that LC27, LC67, and LM can alleviate alcoholic steatosis by inhibiting LPS-mediated NF-κB activation through restoration of the disturbed gut microbiota.
30010169	4	5	theme	LC67	672:675	arg1	administration					648:661	Oral administration	643:661	Oral administration of LC27, LC67, or LM in mice	643:690	Oral administration of LC27, LC67, or LM in mice reduced ethanol-induced ALT, AST, TG, and TC levels in the blood and liver.
30010169	2	6	from	steatosis	466:474	arg1	mice					479:482	mice	479:482	mice	479:482	In the present study, we investigated the effects of Bifidobacterium longum LC67, Lactobacillus plantarum LC27, and their mixture (LM) against ethanol-induced steatosis in mice.
30010169	7	7	theme	lipopolysaccharide	1316:1333	arg1	levels					1335:1340	blood lipopolysaccharide levels	1310:1340	blood lipopolysaccharide levels	1310:1340	Moreover, treatment with LC27, LC67, or LM restored the ethanol-disturbed gut microbiota composition, such as the increased population of Proteobacteria, and inhibited fecal and blood lipopolysaccharide levels.
30010169	5	8	from	expression	849:858	arg1	liver					867:871	the liver	863:871	the liver	863:871	These also suppressed ethanol-induced NF-κB activation and α-smooth muscle actin expression in the liver and increased ethanol-suppressed AMPK activation.
30010169	5	9	theme	muscle	836:841	arg1	actin					843:847	α-smooth muscle actin	827:847	α-smooth muscle actin expression in the liver	827:871	These also suppressed ethanol-induced NF-κB activation and α-smooth muscle actin expression in the liver and increased ethanol-suppressed AMPK activation.
30010169	4	10	theme	ALT	716:718	arg1	levels					737:742	ethanol-induced ALT, AST, TG, and TC levels	700:742	ethanol-induced ALT, AST, TG, and TC levels in the blood and liver	700:765	Oral administration of LC27, LC67, or LM in mice reduced ethanol-induced ALT, AST, TG, and TC levels in the blood and liver.
30010169	7	11	theme	microbiota	1210:1219	arg1	composition					1221:1231	the ethanol-disturbed gut microbiota composition	1184:1231	the ethanol-disturbed gut microbiota composition	1184:1231	Moreover, treatment with LC27, LC67, or LM restored the ethanol-disturbed gut microbiota composition, such as the increased population of Proteobacteria, and inhibited fecal and blood lipopolysaccharide levels.
30010169	7	12	theme	increased	1246:1254	arg1	population					1256:1265	the increased population	1242:1265	the increased population of Proteobacteria	1242:1283	Moreover, treatment with LC27, LC67, or LM restored the ethanol-disturbed gut microbiota composition, such as the increased population of Proteobacteria, and inhibited fecal and blood lipopolysaccharide levels.
30010169	3	13	theme	TG	549:550	arg1	levels					580:585	ALT, AST, TG, TC, and lipopolysaccharide levels	539:585	ALT, AST, TG, TC, and lipopolysaccharide levels	539:585	Exposure to ethanol caused liver damage: it increased ALT, AST, TG, TC, and lipopolysaccharide levels in the blood and induced NF-κB activation in the liver.
30010169	2	14	theme	present	314:320	arg1	study					322:326	the present study	310:326	the present study	310:326	In the present study, we investigated the effects of Bifidobacterium longum LC67, Lactobacillus plantarum LC27, and their mixture (LM) against ethanol-induced steatosis in mice.
30010169	6	15	theme	tight	1074:1078	arg1	expression					1097:1106	tight junction protein expression	1074:1106	tight junction protein expression in the liver and colon	1074:1129	Treatment with LC27, LC67, or LM increased ethanol-suppressed alcohol dehydrogenase and acetaldehyde dehydrogenase activities in the liver, as well as tight junction protein expression in the liver and colon.
30010169	0	16	theme	NF-κB	125:129	arg1	activation					131:140	LPS-mediated NF-κB activation	112:140	LPS-mediated NF-κB activation	112:140	Lactobacillus plantarum LC27 and Bifidobacterium longum LC67 mitigate alcoholic steatosis in mice by inhibiting LPS-mediated NF-κB activation through restoration of the disturbed gut microbiota.
30010169	3	17	theme	liver	512:516	arg1	damage					518:523	liver damage	512:523	liver damage	512:523	Exposure to ethanol caused liver damage: it increased ALT, AST, TG, TC, and lipopolysaccharide levels in the blood and induced NF-κB activation in the liver.
30010169	6	18	theme	acetaldehyde	1011:1022	arg1	dehydrogenase					1024:1036	acetaldehyde dehydrogenase	1011:1036	acetaldehyde dehydrogenase	1011:1036	Treatment with LC27, LC67, or LM increased ethanol-suppressed alcohol dehydrogenase and acetaldehyde dehydrogenase activities in the liver, as well as tight junction protein expression in the liver and colon.
30010169	4	19	from	administration	648:661	arg1	mice					687:690	mice	687:690	mice	687:690	Oral administration of LC27, LC67, or LM in mice reduced ethanol-induced ALT, AST, TG, and TC levels in the blood and liver.
30010169	4	20	theme	Oral	643:646	arg1	administration					648:661	Oral administration	643:661	Oral administration of LC27, LC67, or LM in mice	643:690	Oral administration of LC27, LC67, or LM in mice reduced ethanol-induced ALT, AST, TG, and TC levels in the blood and liver.
30010169	0	21	theme	LPS-mediated	112:123	arg1	activation					131:140	LPS-mediated NF-κB activation	112:140	LPS-mediated NF-κB activation	112:140	Lactobacillus plantarum LC27 and Bifidobacterium longum LC67 mitigate alcoholic steatosis in mice by inhibiting LPS-mediated NF-κB activation through restoration of the disturbed gut microbiota.
30010169	3	22	theme	AST	544:546	arg1	levels					580:585	ALT, AST, TG, TC, and lipopolysaccharide levels	539:585	ALT, AST, TG, TC, and lipopolysaccharide levels	539:585	Exposure to ethanol caused liver damage: it increased ALT, AST, TG, TC, and lipopolysaccharide levels in the blood and induced NF-κB activation in the liver.
30010169	1	23	theme	Long-term	195:203	arg1	exposure					205:212	Long-term exposure	195:212	Long-term exposure to ethanol	195:223	Long-term exposure to ethanol simultaneously causes gastrointestinal inflammation, liver injury, and steatosis.
30010169	0	24	theme	plantarum	14:22	arg1	LC67					56:59	Lactobacillus plantarum LC27 and Bifidobacterium longum LC67	0:59	Lactobacillus plantarum LC27 and Bifidobacterium longum LC67	0:59	Lactobacillus plantarum LC27 and Bifidobacterium longum LC67 mitigate alcoholic steatosis in mice by inhibiting LPS-mediated NF-κB activation through restoration of the disturbed gut microbiota.
30010169	6	25	theme	dehydrogenase	993:1005	arg1	activities					1038:1047	ethanol-suppressed alcohol dehydrogenase and acetaldehyde dehydrogenase activities	966:1047	ethanol-suppressed alcohol dehydrogenase and acetaldehyde dehydrogenase activities in the liver	966:1060	Treatment with LC27, LC67, or LM increased ethanol-suppressed alcohol dehydrogenase and acetaldehyde dehydrogenase activities in the liver, as well as tight junction protein expression in the liver and colon.
30010169	4	26	theme	ethanol-induced	700:714	arg1	levels					737:742	ethanol-induced ALT, AST, TG, and TC levels	700:742	ethanol-induced ALT, AST, TG, and TC levels in the blood and liver	700:765	Oral administration of LC27, LC67, or LM in mice reduced ethanol-induced ALT, AST, TG, and TC levels in the blood and liver.
30010169	9	27	theme	LPS-mediated	1578:1589	arg1	activation					1597:1606	LPS-mediated NF-κB activation	1578:1606	LPS-mediated NF-κB activation	1578:1606	These findings suggest that LC27, LC67, and LM can alleviate alcoholic steatosis by inhibiting LPS-mediated NF-κB activation through restoration of the disturbed gut microbiota.
30010169	5	28	theme	ethanol-suppressed	887:904	arg1	activation					911:920	ethanol-suppressed AMPK activation	887:920	ethanol-suppressed AMPK activation	887:920	These also suppressed ethanol-induced NF-κB activation and α-smooth muscle actin expression in the liver and increased ethanol-suppressed AMPK activation.
30010169	6	29	dep	liver	1115:1119	arg1	the					1111:1113	the	1111:1113	the	1111:1113	Treatment with LC27, LC67, or LM increased ethanol-suppressed alcohol dehydrogenase and acetaldehyde dehydrogenase activities in the liver, as well as tight junction protein expression in the liver and colon.
30010169	2	30	theme	ethanol-induced	450:464	arg1	steatosis					466:474	ethanol-induced steatosis	450:474	ethanol-induced steatosis in mice	450:482	In the present study, we investigated the effects of Bifidobacterium longum LC67, Lactobacillus plantarum LC27, and their mixture (LM) against ethanol-induced steatosis in mice.
30010169	8	31	theme	ethanol-	1427:1434	arg1	cells					1476:1480	ethanol- or lipopolysaccharide-stimulated Caco-2 cells	1427:1480	cells	1476:1480	These inhibited NF-κB activation and increased tight junction protein expression in ethanol- or lipopolysaccharide-stimulated Caco-2 cells.
30010169	4	32	theme	LM	681:682	arg1	administration					648:661	Oral administration	643:661	Oral administration of LC27, LC67, or LM in mice	643:690	Oral administration of LC27, LC67, or LM in mice reduced ethanol-induced ALT, AST, TG, and TC levels in the blood and liver.
30010169	5	33	theme	AMPK	906:909	arg1	activation					911:920	ethanol-suppressed AMPK activation	887:920	ethanol-suppressed AMPK activation	887:920	These also suppressed ethanol-induced NF-κB activation and α-smooth muscle actin expression in the liver and increased ethanol-suppressed AMPK activation.
30010169	8	34	theme	NF-κB	1359:1363	arg1	activation					1365:1374	NF-κB activation	1359:1374	NF-κB activation	1359:1374	These inhibited NF-κB activation and increased tight junction protein expression in ethanol- or lipopolysaccharide-stimulated Caco-2 cells.
30010169	6	35	theme	dehydrogenase	1024:1036	arg1	activities					1038:1047	ethanol-suppressed alcohol dehydrogenase and acetaldehyde dehydrogenase activities	966:1047	ethanol-suppressed alcohol dehydrogenase and acetaldehyde dehydrogenase activities in the liver	966:1060	Treatment with LC27, LC67, or LM increased ethanol-suppressed alcohol dehydrogenase and acetaldehyde dehydrogenase activities in the liver, as well as tight junction protein expression in the liver and colon.
30010169	3	36	theme	TC	553:554	arg1	levels					580:585	ALT, AST, TG, TC, and lipopolysaccharide levels	539:585	ALT, AST, TG, TC, and lipopolysaccharide levels	539:585	Exposure to ethanol caused liver damage: it increased ALT, AST, TG, TC, and lipopolysaccharide levels in the blood and induced NF-κB activation in the liver.
30010169	5	37	theme	α-smooth	827:834	arg1	actin					843:847	α-smooth muscle actin	827:847	α-smooth muscle actin expression in the liver	827:871	These also suppressed ethanol-induced NF-κB activation and α-smooth muscle actin expression in the liver and increased ethanol-suppressed AMPK activation.
30010169	0	38	theme	longum	49:54	arg1	LC67					56:59	Lactobacillus plantarum LC27 and Bifidobacterium longum LC67	0:59	Lactobacillus plantarum LC27 and Bifidobacterium longum LC67	0:59	Lactobacillus plantarum LC27 and Bifidobacterium longum LC67 mitigate alcoholic steatosis in mice by inhibiting LPS-mediated NF-κB activation through restoration of the disturbed gut microbiota.
30010169	9	39	theme	alcoholic	1544:1552	arg1	steatosis					1554:1562	alcoholic steatosis	1544:1562	alcoholic steatosis	1544:1562	These findings suggest that LC27, LC67, and LM can alleviate alcoholic steatosis by inhibiting LPS-mediated NF-κB activation through restoration of the disturbed gut microbiota.
30010169	7	40	theme	ethanol-disturbed	1188:1204	arg1	composition					1221:1231	the ethanol-disturbed gut microbiota composition	1184:1231	the ethanol-disturbed gut microbiota composition	1184:1231	Moreover, treatment with LC27, LC67, or LM restored the ethanol-disturbed gut microbiota composition, such as the increased population of Proteobacteria, and inhibited fecal and blood lipopolysaccharide levels.
30010169	7	41	with	treatment	1142:1150	arg1	LC27					1157:1160	LC27	1157:1160	LC27	1157:1160	Moreover, treatment with LC27, LC67, or LM restored the ethanol-disturbed gut microbiota composition, such as the increased population of Proteobacteria, and inhibited fecal and blood lipopolysaccharide levels.
30010169	7	41	with	treatment	1142:1150	arg1	LC67					1163:1166	LC67	1163:1166	LC67	1163:1166	Moreover, treatment with LC27, LC67, or LM restored the ethanol-disturbed gut microbiota composition, such as the increased population of Proteobacteria, and inhibited fecal and blood lipopolysaccharide levels.
30010169	7	41	with	treatment	1142:1150	arg1	LM					1172:1173	LM	1172:1173	LM	1172:1173	Moreover, treatment with LC27, LC67, or LM restored the ethanol-disturbed gut microbiota composition, such as the increased population of Proteobacteria, and inhibited fecal and blood lipopolysaccharide levels.
30010169	7	42	theme	Proteobacteria	1270:1283	arg1	population					1256:1265	the increased population	1242:1265	the increased population of Proteobacteria	1242:1283	Moreover, treatment with LC27, LC67, or LM restored the ethanol-disturbed gut microbiota composition, such as the increased population of Proteobacteria, and inhibited fecal and blood lipopolysaccharide levels.
30010169	4	43	dep	blood	751:755	arg1	the					747:749	the	747:749	the	747:749	Oral administration of LC27, LC67, or LM in mice reduced ethanol-induced ALT, AST, TG, and TC levels in the blood and liver.
30010169	5	44	theme	ethanol-induced	790:804	arg1	activation					812:821	ethanol-induced NF-κB activation	790:821	ethanol-induced NF-κB activation	790:821	These also suppressed ethanol-induced NF-κB activation and α-smooth muscle actin expression in the liver and increased ethanol-suppressed AMPK activation.
30010169	0	45	from	steatosis	80:88	arg1	mice					93:96	mice	93:96	mice	93:96	Lactobacillus plantarum LC27 and Bifidobacterium longum LC67 mitigate alcoholic steatosis in mice by inhibiting LPS-mediated NF-κB activation through restoration of the disturbed gut microbiota.
30010169	9	46	theme	NF-κB	1591:1595	arg1	activation					1597:1606	LPS-mediated NF-κB activation	1578:1606	LPS-mediated NF-κB activation	1578:1606	These findings suggest that LC27, LC67, and LM can alleviate alcoholic steatosis by inhibiting LPS-mediated NF-κB activation through restoration of the disturbed gut microbiota.
30010169	1	47	theme	gastrointestinal	247:262	arg1	inflammation					264:275	gastrointestinal inflammation	247:275	gastrointestinal inflammation	247:275	Long-term exposure to ethanol simultaneously causes gastrointestinal inflammation, liver injury, and steatosis.
30010169	0	48	theme	disturbed	169:177	arg1	microbiota					183:192	the disturbed gut microbiota	165:192	the disturbed gut microbiota	165:192	Lactobacillus plantarum LC27 and Bifidobacterium longum LC67 mitigate alcoholic steatosis in mice by inhibiting LPS-mediated NF-κB activation through restoration of the disturbed gut microbiota.
30010169	4	49	theme	AST	721:723	arg1	levels					737:742	ethanol-induced ALT, AST, TG, and TC levels	700:742	ethanol-induced ALT, AST, TG, and TC levels in the blood and liver	700:765	Oral administration of LC27, LC67, or LM in mice reduced ethanol-induced ALT, AST, TG, and TC levels in the blood and liver.
30010169	3	50	theme	lipopolysaccharide	561:578	arg1	levels					580:585	ALT, AST, TG, TC, and lipopolysaccharide levels	539:585	ALT, AST, TG, TC, and lipopolysaccharide levels	539:585	Exposure to ethanol caused liver damage: it increased ALT, AST, TG, TC, and lipopolysaccharide levels in the blood and induced NF-κB activation in the liver.
30010169	2	51	theme	longum	376:381	arg1	effects					349:355	the effects	345:355	the effects of Bifidobacterium longum LC67, Lactobacillus plantarum LC27, and their mixture (LM) against ethanol-induced steatosis in mice	345:482	In the present study, we investigated the effects of Bifidobacterium longum LC67, Lactobacillus plantarum LC27, and their mixture (LM) against ethanol-induced steatosis in mice.
30010169	6	52	with	Treatment	923:931	arg1	LC67					944:947	LC67	944:947	LC67	944:947	Treatment with LC27, LC67, or LM increased ethanol-suppressed alcohol dehydrogenase and acetaldehyde dehydrogenase activities in the liver, as well as tight junction protein expression in the liver and colon.
30010169	6	52	with	Treatment	923:931	arg1	LM					953:954	LM	953:954	LM	953:954	Treatment with LC27, LC67, or LM increased ethanol-suppressed alcohol dehydrogenase and acetaldehyde dehydrogenase activities in the liver, as well as tight junction protein expression in the liver and colon.
30010169	6	52	with	Treatment	923:931	arg1	LC27					938:941	LC27	938:941	LC27	938:941	Treatment with LC27, LC67, or LM increased ethanol-suppressed alcohol dehydrogenase and acetaldehyde dehydrogenase activities in the liver, as well as tight junction protein expression in the liver and colon.
30010169	8	53	theme	Caco-2	1469:1474	arg1	cells					1476:1480	ethanol- or lipopolysaccharide-stimulated Caco-2 cells	1427:1480	cells	1476:1480	These inhibited NF-κB activation and increased tight junction protein expression in ethanol- or lipopolysaccharide-stimulated Caco-2 cells.
30010169	8	54	theme	lipopolysaccharide-stimulated	1439:1467	arg1	cells					1476:1480	ethanol- or lipopolysaccharide-stimulated Caco-2 cells	1427:1480	cells	1476:1480	These inhibited NF-κB activation and increased tight junction protein expression in ethanol- or lipopolysaccharide-stimulated Caco-2 cells.
30010169	6	55	from	expression	1097:1106	arg1	liver					1115:1119	liver	1115:1119	liver	1115:1119	Treatment with LC27, LC67, or LM increased ethanol-suppressed alcohol dehydrogenase and acetaldehyde dehydrogenase activities in the liver, as well as tight junction protein expression in the liver and colon.
30010169	6	55	from	expression	1097:1106	arg1	colon					1125:1129	colon	1125:1129	colon	1125:1129	Treatment with LC27, LC67, or LM increased ethanol-suppressed alcohol dehydrogenase and acetaldehyde dehydrogenase activities in the liver, as well as tight junction protein expression in the liver and colon.
30010169	6	55	from	expression	1097:1106	arg1	liver					1056:1060	the liver	1052:1060	the liver	1052:1060	Treatment with LC27, LC67, or LM increased ethanol-suppressed alcohol dehydrogenase and acetaldehyde dehydrogenase activities in the liver, as well as tight junction protein expression in the liver and colon.
30010169	6	56	theme	protein	1089:1095	arg1	expression					1097:1106	tight junction protein expression	1074:1106	tight junction protein expression in the liver and colon	1074:1129	Treatment with LC27, LC67, or LM increased ethanol-suppressed alcohol dehydrogenase and acetaldehyde dehydrogenase activities in the liver, as well as tight junction protein expression in the liver and colon.
30010169	7	57	theme	gut	1206:1208	arg1	composition					1221:1231	the ethanol-disturbed gut microbiota composition	1184:1231	the ethanol-disturbed gut microbiota composition	1184:1231	Moreover, treatment with LC27, LC67, or LM restored the ethanol-disturbed gut microbiota composition, such as the increased population of Proteobacteria, and inhibited fecal and blood lipopolysaccharide levels.
30010169	0	58	theme	microbiota	183:192	arg1	restoration					150:160	restoration	150:160	restoration of the disturbed gut microbiota	150:192	Lactobacillus plantarum LC27 and Bifidobacterium longum LC67 mitigate alcoholic steatosis in mice by inhibiting LPS-mediated NF-κB activation through restoration of the disturbed gut microbiota.
30010169	8	59	theme	tight	1390:1394	arg1	expression					1413:1422	increased tight junction protein expression	1380:1422	increased tight junction protein expression	1380:1422	These inhibited NF-κB activation and increased tight junction protein expression in ethanol- or lipopolysaccharide-stimulated Caco-2 cells.
30010169	4	60	theme	TC	734:735	arg1	levels					737:742	ethanol-induced ALT, AST, TG, and TC levels	700:742	ethanol-induced ALT, AST, TG, and TC levels in the blood and liver	700:765	Oral administration of LC27, LC67, or LM in mice reduced ethanol-induced ALT, AST, TG, and TC levels in the blood and liver.
30010169	6	61	from	activities	1038:1047	arg1	liver					1115:1119	liver	1115:1119	liver	1115:1119	Treatment with LC27, LC67, or LM increased ethanol-suppressed alcohol dehydrogenase and acetaldehyde dehydrogenase activities in the liver, as well as tight junction protein expression in the liver and colon.
30010169	6	61	from	activities	1038:1047	arg1	colon					1125:1129	colon	1125:1129	colon	1125:1129	Treatment with LC27, LC67, or LM increased ethanol-suppressed alcohol dehydrogenase and acetaldehyde dehydrogenase activities in the liver, as well as tight junction protein expression in the liver and colon.
30010169	6	61	from	activities	1038:1047	arg1	liver					1056:1060	the liver	1052:1060	the liver	1052:1060	Treatment with LC27, LC67, or LM increased ethanol-suppressed alcohol dehydrogenase and acetaldehyde dehydrogenase activities in the liver, as well as tight junction protein expression in the liver and colon.
30010169	6	62	theme	junction	1080:1087	arg1	expression					1097:1106	tight junction protein expression	1074:1106	tight junction protein expression in the liver and colon	1074:1129	Treatment with LC27, LC67, or LM increased ethanol-suppressed alcohol dehydrogenase and acetaldehyde dehydrogenase activities in the liver, as well as tight junction protein expression in the liver and colon.
30010169	6	63	theme	alcohol	985:991	arg1	dehydrogenase					993:1005	ethanol-suppressed alcohol dehydrogenase	966:1005	ethanol-suppressed alcohol dehydrogenase	966:1005	Treatment with LC27, LC67, or LM increased ethanol-suppressed alcohol dehydrogenase and acetaldehyde dehydrogenase activities in the liver, as well as tight junction protein expression in the liver and colon.
30010169	0	64	theme	gut	179:181	arg1	microbiota					183:192	the disturbed gut microbiota	165:192	the disturbed gut microbiota	165:192	Lactobacillus plantarum LC27 and Bifidobacterium longum LC67 mitigate alcoholic steatosis in mice by inhibiting LPS-mediated NF-κB activation through restoration of the disturbed gut microbiota.
30010169	3	65	theme	NF-κB	612:616	arg1	activation					618:627	NF-κB activation	612:627	NF-κB activation	612:627	Exposure to ethanol caused liver damage: it increased ALT, AST, TG, TC, and lipopolysaccharide levels in the blood and induced NF-κB activation in the liver.
30010169	5	66	from	activation	812:821	arg1	liver					867:871	the liver	863:871	the liver	863:871	These also suppressed ethanol-induced NF-κB activation and α-smooth muscle actin expression in the liver and increased ethanol-suppressed AMPK activation.
30010169	4	67	theme	LC27	666:669	arg1	administration					648:661	Oral administration	643:661	Oral administration of LC27, LC67, or LM in mice	643:690	Oral administration of LC27, LC67, or LM in mice reduced ethanol-induced ALT, AST, TG, and TC levels in the blood and liver.
30010169	6	68	theme	ethanol-suppressed	966:983	arg1	dehydrogenase					993:1005	ethanol-suppressed alcohol dehydrogenase	966:1005	ethanol-suppressed alcohol dehydrogenase	966:1005	Treatment with LC27, LC67, or LM increased ethanol-suppressed alcohol dehydrogenase and acetaldehyde dehydrogenase activities in the liver, as well as tight junction protein expression in the liver and colon.
30010169	8	69	theme	increased	1380:1388	arg1	expression					1413:1422	increased tight junction protein expression	1380:1422	increased tight junction protein expression	1380:1422	These inhibited NF-κB activation and increased tight junction protein expression in ethanol- or lipopolysaccharide-stimulated Caco-2 cells.
30010169	2	70	theme	mixture	429:435	arg1	effects					349:355	the effects	345:355	the effects of Bifidobacterium longum LC67, Lactobacillus plantarum LC27, and their mixture (LM) against ethanol-induced steatosis in mice	345:482	In the present study, we investigated the effects of Bifidobacterium longum LC67, Lactobacillus plantarum LC27, and their mixture (LM) against ethanol-induced steatosis in mice.
30010169	8	71	theme	protein	1405:1411	arg1	expression					1413:1422	increased tight junction protein expression	1380:1422	increased tight junction protein expression	1380:1422	These inhibited NF-κB activation and increased tight junction protein expression in ethanol- or lipopolysaccharide-stimulated Caco-2 cells.
30010169	4	72	from	levels	737:742	arg1	blood					751:755	blood	751:755	blood	751:755	Oral administration of LC27, LC67, or LM in mice reduced ethanol-induced ALT, AST, TG, and TC levels in the blood and liver.
30010169	4	72	from	levels	737:742	arg1	liver					761:765	liver	761:765	liver	761:765	Oral administration of LC27, LC67, or LM in mice reduced ethanol-induced ALT, AST, TG, and TC levels in the blood and liver.
30010169	2	73	theme	plantarum	403:411	arg1	effects					349:355	the effects	345:355	the effects of Bifidobacterium longum LC67, Lactobacillus plantarum LC27, and their mixture (LM) against ethanol-induced steatosis in mice	345:482	In the present study, we investigated the effects of Bifidobacterium longum LC67, Lactobacillus plantarum LC27, and their mixture (LM) against ethanol-induced steatosis in mice.
30010169	5	74	theme	NF-κB	806:810	arg1	activation					812:821	ethanol-induced NF-κB activation	790:821	ethanol-induced NF-κB activation	790:821	These also suppressed ethanol-induced NF-κB activation and α-smooth muscle actin expression in the liver and increased ethanol-suppressed AMPK activation.
30010169	1	75	theme	liver	278:282	arg1	injury					284:289	liver injury	278:289	liver injury	278:289	Long-term exposure to ethanol simultaneously causes gastrointestinal inflammation, liver injury, and steatosis.
30010169	0	76	theme	alcoholic	70:78	arg1	steatosis					80:88	alcoholic steatosis	70:88	alcoholic steatosis in mice	70:96	Lactobacillus plantarum LC27 and Bifidobacterium longum LC67 mitigate alcoholic steatosis in mice by inhibiting LPS-mediated NF-κB activation through restoration of the disturbed gut microbiota.
30010169	8	77	theme	junction	1396:1403	arg1	expression					1413:1422	increased tight junction protein expression	1380:1422	increased tight junction protein expression	1380:1422	These inhibited NF-κB activation and increased tight junction protein expression in ethanol- or lipopolysaccharide-stimulated Caco-2 cells.
30010169	3	78	theme	ALT	539:541	arg1	levels					580:585	ALT, AST, TG, TC, and lipopolysaccharide levels	539:585	ALT, AST, TG, TC, and lipopolysaccharide levels	539:585	Exposure to ethanol caused liver damage: it increased ALT, AST, TG, TC, and lipopolysaccharide levels in the blood and induced NF-κB activation in the liver.
30010169	9	79	theme	disturbed	1635:1643	arg1	microbiota					1649:1658	the disturbed gut microbiota	1631:1658	the disturbed gut microbiota	1631:1658	These findings suggest that LC27, LC67, and LM can alleviate alcoholic steatosis by inhibiting LPS-mediated NF-κB activation through restoration of the disturbed gut microbiota.
29437230	7	0	theme	tropical	1593:1600	arg1	perennial					1602:1610	a tropical perennial	1591:1610	a tropical perennial legume consumed in many Asian countries	1591:1650	PRACTICAL APPLICATION Japanese red sword bean (RSB) is a tropical perennial legume consumed in many Asian countries.
29437230	7	0	theme	tropical	1593:1600	arg1	APPLICATION					1546:1556	PRACTICAL APPLICATION Japanese red sword bean (RSB)	1536:1586	PRACTICAL APPLICATION Japanese red sword bean (RSB)	1536:1586	PRACTICAL APPLICATION Japanese red sword bean (RSB) is a tropical perennial legume consumed in many Asian countries.
29437230	3	1	theme	acid	608:611	arg1	composition					613:623	amino acid composition	602:623	amino acid composition	602:623	The amino acid composition and sequences of RSB lectin were similar to those of Con A. RSB lectin showed specificity to mannose, glucose, maltose, methyl-D-mannoside, and thyroglobulin, but not rhamnose, using mouse, sheep, and rabbit erythrocytes.
29437230	5	2	theme	-treated	1225:1232	arg1	populations					1234:1244	the control (0 μg/mL) and interleukin 2 (IL-2) (25 U)-treated populations	1172:1244	the control (0 μg/mL) and interleukin 2 (IL-2) (25 U)-treated populations	1172:1244	Notably, while treatment with RSB lectin and Con A (0.01 and 0.1 μg/mL) promoted similar levels of splenic NK cell activity, which were higher than that observed in the control (0 μg/mL) and interleukin 2 (IL-2) (25 U)-treated populations, RBS lectin exerted a significantly stronger anti-proliferative effect than Con A at a concentration of 125.0 μg per well.
29437230	2	3	theme	melanoma	531:538	arg1	proliferation					545:557	B16 melanoma cell proliferation	527:557	B16 melanoma cell proliferation	527:557	RSB lectin was purified using maltamyl-Sepharose 4B and subjected to amino acid composition and partial amino acid sequencing analyses, and evaluated for blood and carbohydrate specificity, mitogenic activity, splenic natural killer (NK) cell activity, and its effect on B16 melanoma cell proliferation, compared with Concanavalin A (Con A).
29437230	4	4	theme	greater	934:940	arg1	temperatures					921:932	temperatures	921:932	temperatures greater than 70 °C	921:951	Compared with Con A, RSB lectin showed low resistance to proteases and to temperatures greater than 70 °C, but high mitogenic activity for mouse splenic cells.
29437230	3	5	theme	lectin	646:651	arg1	sequences					629:637	sequences	629:637	sequences	629:637	The amino acid composition and sequences of RSB lectin were similar to those of Con A. RSB lectin showed specificity to mannose, glucose, maltose, methyl-D-mannoside, and thyroglobulin, but not rhamnose, using mouse, sheep, and rabbit erythrocytes.
29437230	3	5	theme	lectin	646:651	arg1	composition					613:623	amino acid composition	602:623	amino acid composition	602:623	The amino acid composition and sequences of RSB lectin were similar to those of Con A. RSB lectin showed specificity to mannose, glucose, maltose, methyl-D-mannoside, and thyroglobulin, but not rhamnose, using mouse, sheep, and rabbit erythrocytes.
29437230	8	6	theme	RSB	1653:1655	arg1	lectin					1657:1662	RSB lectin	1653:1662	RSB lectin	1653:1662	RSB lectin shows specificity to mannose, glucose, maltose, methyl-d-mannoside, and thyroglobulin, but not to rhamnose, using mouse, sheep, and rabbit erythrocytes.
29437230	2	7	theme	Concanavalin	574:585	arg1	A					587:587	Concanavalin A	574:587	Concanavalin A (Con A)	574:595	RSB lectin was purified using maltamyl-Sepharose 4B and subjected to amino acid composition and partial amino acid sequencing analyses, and evaluated for blood and carbohydrate specificity, mitogenic activity, splenic natural killer (NK) cell activity, and its effect on B16 melanoma cell proliferation, compared with Concanavalin A (Con A).
29437230	2	7	theme	Concanavalin	574:585	arg1	A					594:594	Con A	590:594	Con A	590:594	RSB lectin was purified using maltamyl-Sepharose 4B and subjected to amino acid composition and partial amino acid sequencing analyses, and evaluated for blood and carbohydrate specificity, mitogenic activity, splenic natural killer (NK) cell activity, and its effect on B16 melanoma cell proliferation, compared with Concanavalin A (Con A).
29437230	4	8	theme	splenic	992:998	arg1	cells					1000:1004	mouse splenic cells	986:1004	mouse splenic cells	986:1004	Compared with Con A, RSB lectin showed low resistance to proteases and to temperatures greater than 70 °C, but high mitogenic activity for mouse splenic cells.
29437230	10	9	contain	has	2126:2128	arg1	lectin					2119:2124	RSB lectin	2115:2124	RSB lectin	2115:2124	Thus, RSB lectin has the potential to be used as a bioactive protein in medical research.
29437230	10	9	contain	has	2126:2128	arg2	potential					2134:2142	the potential to be used as a bioactive protein in medical research	2130:2196	the potential to be used as a bioactive protein in medical research	2130:2196	Thus, RSB lectin has the potential to be used as a bioactive protein in medical research.
29437230	1	10	theme	chemical	147:154	arg1	profile					171:177	the chemical and biological profile	143:177	the chemical and biological profile of lectin isolated from Japanese red sword beans (Canavalia gladiata; RSBs)	143:253	In this study, we investigated the chemical and biological profile of lectin isolated from Japanese red sword beans (Canavalia gladiata; RSBs).
29437230	1	11	theme	biological	160:169	arg1	profile					171:177	the chemical and biological profile	143:177	the chemical and biological profile of lectin isolated from Japanese red sword beans (Canavalia gladiata; RSBs)	143:253	In this study, we investigated the chemical and biological profile of lectin isolated from Japanese red sword beans (Canavalia gladiata; RSBs).
29437230	7	12	theme	red	1567:1569	arg1	RSB					1583:1585	RSB	1583:1585	RSB	1583:1585	PRACTICAL APPLICATION Japanese red sword bean (RSB) is a tropical perennial legume consumed in many Asian countries.
29437230	7	12	theme	red	1567:1569	arg1	bean					1577:1580	Japanese red sword bean	1558:1580	PRACTICAL APPLICATION Japanese red sword bean (RSB)	1536:1586	PRACTICAL APPLICATION Japanese red sword bean (RSB) is a tropical perennial legume consumed in many Asian countries.
29437230	5	13	with	treatment	1022:1030	arg1	lectin					1041:1046	RSB lectin	1037:1046	RSB lectin	1037:1046	Notably, while treatment with RSB lectin and Con A (0.01 and 0.1 μg/mL) promoted similar levels of splenic NK cell activity, which were higher than that observed in the control (0 μg/mL) and interleukin 2 (IL-2) (25 U)-treated populations, RBS lectin exerted a significantly stronger anti-proliferative effect than Con A at a concentration of 125.0 μg per well.
29437230	5	13	with	treatment	1022:1030	arg1	A					1056:1056	Con A	1052:1056	Con A (0.01 and 0.1 μg/mL)	1052:1077	Notably, while treatment with RSB lectin and Con A (0.01 and 0.1 μg/mL) promoted similar levels of splenic NK cell activity, which were higher than that observed in the control (0 μg/mL) and interleukin 2 (IL-2) (25 U)-treated populations, RBS lectin exerted a significantly stronger anti-proliferative effect than Con A at a concentration of 125.0 μg per well.
29437230	9	14	theme	mouse	1937:1941	arg1	cells					1951:1955	mouse splenic cells	1937:1955	mouse splenic cells	1937:1955	RSB lectin exhibits similarities to Concanavalin A in amino acid composition and sequence, shows mitogenic activity for mouse splenic cells and strong anti-proliferative activity for B16 melanoma cells, and also enhances the activity of splenic natural killer (NK) cells against YAC-1 cells.
29437230	2	15	theme	RSB	256:258	arg1	lectin					260:265	RSB lectin	256:265	RSB lectin	256:265	RSB lectin was purified using maltamyl-Sepharose 4B and subjected to amino acid composition and partial amino acid sequencing analyses, and evaluated for blood and carbohydrate specificity, mitogenic activity, splenic natural killer (NK) cell activity, and its effect on B16 melanoma cell proliferation, compared with Concanavalin A (Con A).
29437230	2	16	theme	cell	494:497	arg1	activity					499:506	splenic natural killer (NK) cell activity	466:506	splenic natural killer (NK) cell activity	466:506	RSB lectin was purified using maltamyl-Sepharose 4B and subjected to amino acid composition and partial amino acid sequencing analyses, and evaluated for blood and carbohydrate specificity, mitogenic activity, splenic natural killer (NK) cell activity, and its effect on B16 melanoma cell proliferation, compared with Concanavalin A (Con A).
29437230	3	17	theme	RSB	685:687	arg1	lectin					689:694	Con A. RSB lectin	678:694	Con A. RSB lectin showed specificity to mannose, glucose, maltose, methyl-D-mannoside, and thyroglobulin, but not rhamnose	678:799	The amino acid composition and sequences of RSB lectin were similar to those of Con A. RSB lectin showed specificity to mannose, glucose, maltose, methyl-D-mannoside, and thyroglobulin, but not rhamnose, using mouse, sheep, and rabbit erythrocytes.
29437230	9	18	theme	anti-proliferative	1968:1985	arg1	activity					1987:1994	strong anti-proliferative activity	1961:1994	strong anti-proliferative activity for B16 melanoma cells	1961:2017	RSB lectin exhibits similarities to Concanavalin A in amino acid composition and sequence, shows mitogenic activity for mouse splenic cells and strong anti-proliferative activity for B16 melanoma cells, and also enhances the activity of splenic natural killer (NK) cells against YAC-1 cells.
29437230	6	19	used	used	1486:1489	arg2	agent					1529:1533	a potential cancer chemopreventive agent	1494:1533	a potential cancer chemopreventive agent	1494:1533	Overall, our results show that RSB lectin might exert immunological effects on mouse splenic cells and could thus be used as a potential cancer chemopreventive agent.
29437230	6	19	used	used	1486:1489	arg2	lectin					1404:1409	RSB lectin	1400:1409	RSB lectin	1400:1409	Overall, our results show that RSB lectin might exert immunological effects on mouse splenic cells and could thus be used as a potential cancer chemopreventive agent.
29437230	3	20	theme	Con	678:680	arg1	lectin					689:694	Con A. RSB lectin	678:694	Con A. RSB lectin showed specificity to mannose, glucose, maltose, methyl-D-mannoside, and thyroglobulin, but not rhamnose	678:799	The amino acid composition and sequences of RSB lectin were similar to those of Con A. RSB lectin showed specificity to mannose, glucose, maltose, methyl-D-mannoside, and thyroglobulin, but not rhamnose, using mouse, sheep, and rabbit erythrocytes.
29437230	7	21	theme	PRACTICAL	1536:1544	arg1	perennial					1602:1610	a tropical perennial	1591:1610	a tropical perennial legume consumed in many Asian countries	1591:1650	PRACTICAL APPLICATION Japanese red sword bean (RSB) is a tropical perennial legume consumed in many Asian countries.
29437230	7	21	theme	PRACTICAL	1536:1544	arg1	APPLICATION					1546:1556	PRACTICAL APPLICATION Japanese red sword bean (RSB)	1536:1586	PRACTICAL APPLICATION Japanese red sword bean (RSB)	1536:1586	PRACTICAL APPLICATION Japanese red sword bean (RSB) is a tropical perennial legume consumed in many Asian countries.
29437230	1	22	dep	gladiata	239:246	arg1	RSBs					249:252	RSBs	249:252	Canavalia gladiata; RSBs	229:252	In this study, we investigated the chemical and biological profile of lectin isolated from Japanese red sword beans (Canavalia gladiata; RSBs).
29437230	5	23	theme	activity	1122:1129	arg1	levels					1096:1101	similar levels	1088:1101	similar levels	1088:1101	Notably, while treatment with RSB lectin and Con A (0.01 and 0.1 μg/mL) promoted similar levels of splenic NK cell activity, which were higher than that observed in the control (0 μg/mL) and interleukin 2 (IL-2) (25 U)-treated populations, RBS lectin exerted a significantly stronger anti-proliferative effect than Con A at a concentration of 125.0 μg per well.
29437230	5	24	dep	-treated	1225:1232	arg1	IL-2					1213:1216	IL-2	1213:1216	IL-2	1213:1216	Notably, while treatment with RSB lectin and Con A (0.01 and 0.1 μg/mL) promoted similar levels of splenic NK cell activity, which were higher than that observed in the control (0 μg/mL) and interleukin 2 (IL-2) (25 U)-treated populations, RBS lectin exerted a significantly stronger anti-proliferative effect than Con A at a concentration of 125.0 μg per well.
29437230	5	24	dep	-treated	1225:1232	arg1	0 μg/mL					1185:1191	0 μg/mL	1185:1191	0 μg/mL	1185:1191	Notably, while treatment with RSB lectin and Con A (0.01 and 0.1 μg/mL) promoted similar levels of splenic NK cell activity, which were higher than that observed in the control (0 μg/mL) and interleukin 2 (IL-2) (25 U)-treated populations, RBS lectin exerted a significantly stronger anti-proliferative effect than Con A at a concentration of 125.0 μg per well.
29437230	5	24	dep	-treated	1225:1232	arg1	interleukin					1198:1208	interleukin 2	1198:1210	interleukin 2 (IL-2)	1198:1217	Notably, while treatment with RSB lectin and Con A (0.01 and 0.1 μg/mL) promoted similar levels of splenic NK cell activity, which were higher than that observed in the control (0 μg/mL) and interleukin 2 (IL-2) (25 U)-treated populations, RBS lectin exerted a significantly stronger anti-proliferative effect than Con A at a concentration of 125.0 μg per well.
29437230	5	24	dep	-treated	1225:1232	arg1	control					1176:1182	the control	1172:1182	the control (0 μg/mL)	1172:1192	Notably, while treatment with RSB lectin and Con A (0.01 and 0.1 μg/mL) promoted similar levels of splenic NK cell activity, which were higher than that observed in the control (0 μg/mL) and interleukin 2 (IL-2) (25 U)-treated populations, RBS lectin exerted a significantly stronger anti-proliferative effect than Con A at a concentration of 125.0 μg per well.
29437230	2	25	theme	splenic	466:472	arg1	killer					482:487	splenic natural killer	466:487	splenic natural killer (NK) cell activity	466:506	RSB lectin was purified using maltamyl-Sepharose 4B and subjected to amino acid composition and partial amino acid sequencing analyses, and evaluated for blood and carbohydrate specificity, mitogenic activity, splenic natural killer (NK) cell activity, and its effect on B16 melanoma cell proliferation, compared with Concanavalin A (Con A).
29437230	2	25	theme	splenic	466:472	arg1	NK					490:491	NK	490:491	NK	490:491	RSB lectin was purified using maltamyl-Sepharose 4B and subjected to amino acid composition and partial amino acid sequencing analyses, and evaluated for blood and carbohydrate specificity, mitogenic activity, splenic natural killer (NK) cell activity, and its effect on B16 melanoma cell proliferation, compared with Concanavalin A (Con A).
29437230	6	26	theme	splenic	1454:1460	arg1	cells					1462:1466	mouse splenic cells	1448:1466	mouse splenic cells	1448:1466	Overall, our results show that RSB lectin might exert immunological effects on mouse splenic cells and could thus be used as a potential cancer chemopreventive agent.
29437230	9	27	theme	B16	2000:2002	arg1	cells					2013:2017	B16 melanoma cells	2000:2017	B16 melanoma cells	2000:2017	RSB lectin exhibits similarities to Concanavalin A in amino acid composition and sequence, shows mitogenic activity for mouse splenic cells and strong anti-proliferative activity for B16 melanoma cells, and also enhances the activity of splenic natural killer (NK) cells against YAC-1 cells.
29437230	3	28	dep	similar	658:664	arg1	using					802:806	using	802:806	using mouse, sheep, and rabbit erythrocytes	802:844	The amino acid composition and sequences of RSB lectin were similar to those of Con A. RSB lectin showed specificity to mannose, glucose, maltose, methyl-D-mannoside, and thyroglobulin, but not rhamnose, using mouse, sheep, and rabbit erythrocytes.
29437230	10	29	theme	RSB	2115:2117	arg1	lectin					2119:2124	RSB lectin	2115:2124	RSB lectin	2115:2124	Thus, RSB lectin has the potential to be used as a bioactive protein in medical research.
29437230	5	30	theme	stronger	1282:1289	arg1	effect					1310:1315	a significantly stronger anti-proliferative effect	1266:1315	a significantly stronger anti-proliferative effect than Con A	1266:1326	Notably, while treatment with RSB lectin and Con A (0.01 and 0.1 μg/mL) promoted similar levels of splenic NK cell activity, which were higher than that observed in the control (0 μg/mL) and interleukin 2 (IL-2) (25 U)-treated populations, RBS lectin exerted a significantly stronger anti-proliferative effect than Con A at a concentration of 125.0 μg per well.
29437230	5	31	dep	A	1056:1056	arg1	0.01					1059:1062	0.01	1059:1062	0.01	1059:1062	Notably, while treatment with RSB lectin and Con A (0.01 and 0.1 μg/mL) promoted similar levels of splenic NK cell activity, which were higher than that observed in the control (0 μg/mL) and interleukin 2 (IL-2) (25 U)-treated populations, RBS lectin exerted a significantly stronger anti-proliferative effect than Con A at a concentration of 125.0 μg per well.
29437230	5	31	dep	A	1056:1056	arg1	0.1 μg/mL					1068:1076	0.1 μg/mL	1068:1076	0.1 μg/mL	1068:1076	Notably, while treatment with RSB lectin and Con A (0.01 and 0.1 μg/mL) promoted similar levels of splenic NK cell activity, which were higher than that observed in the control (0 μg/mL) and interleukin 2 (IL-2) (25 U)-treated populations, RBS lectin exerted a significantly stronger anti-proliferative effect than Con A at a concentration of 125.0 μg per well.
29437230	6	32	theme	mouse	1448:1452	arg1	cells					1462:1466	mouse splenic cells	1448:1466	mouse splenic cells	1448:1466	Overall, our results show that RSB lectin might exert immunological effects on mouse splenic cells and could thus be used as a potential cancer chemopreventive agent.
29437230	2	33	theme	Con	590:592	arg1	A					587:587	Concanavalin A	574:587	Concanavalin A (Con A)	574:595	RSB lectin was purified using maltamyl-Sepharose 4B and subjected to amino acid composition and partial amino acid sequencing analyses, and evaluated for blood and carbohydrate specificity, mitogenic activity, splenic natural killer (NK) cell activity, and its effect on B16 melanoma cell proliferation, compared with Concanavalin A (Con A).
29437230	2	33	theme	Con	590:592	arg1	A					594:594	Con A	590:594	Con A	590:594	RSB lectin was purified using maltamyl-Sepharose 4B and subjected to amino acid composition and partial amino acid sequencing analyses, and evaluated for blood and carbohydrate specificity, mitogenic activity, splenic natural killer (NK) cell activity, and its effect on B16 melanoma cell proliferation, compared with Concanavalin A (Con A).
29437230	5	34	theme	NK	1114:1115	arg1	activity					1122:1129	splenic NK cell activity	1106:1129	splenic NK cell activity	1106:1129	Notably, while treatment with RSB lectin and Con A (0.01 and 0.1 μg/mL) promoted similar levels of splenic NK cell activity, which were higher than that observed in the control (0 μg/mL) and interleukin 2 (IL-2) (25 U)-treated populations, RBS lectin exerted a significantly stronger anti-proliferative effect than Con A at a concentration of 125.0 μg per well.
29437230	9	35	from	similarities	1837:1848	arg1	composition					1882:1892	amino acid composition	1871:1892	amino acid composition	1871:1892	RSB lectin exhibits similarities to Concanavalin A in amino acid composition and sequence, shows mitogenic activity for mouse splenic cells and strong anti-proliferative activity for B16 melanoma cells, and also enhances the activity of splenic natural killer (NK) cells against YAC-1 cells.
29437230	9	35	from	similarities	1837:1848	arg1	sequence					1898:1905	sequence	1898:1905	sequence	1898:1905	RSB lectin exhibits similarities to Concanavalin A in amino acid composition and sequence, shows mitogenic activity for mouse splenic cells and strong anti-proliferative activity for B16 melanoma cells, and also enhances the activity of splenic natural killer (NK) cells against YAC-1 cells.
29437230	2	36	theme	killer	482:487	arg1	activity					499:506	splenic natural killer (NK) cell activity	466:506	splenic natural killer (NK) cell activity	466:506	RSB lectin was purified using maltamyl-Sepharose 4B and subjected to amino acid composition and partial amino acid sequencing analyses, and evaluated for blood and carbohydrate specificity, mitogenic activity, splenic natural killer (NK) cell activity, and its effect on B16 melanoma cell proliferation, compared with Concanavalin A (Con A).
29437230	6	37	theme	cancer	1506:1511	arg1	lectin					1404:1409	RSB lectin	1400:1409	RSB lectin	1400:1409	Overall, our results show that RSB lectin might exert immunological effects on mouse splenic cells and could thus be used as a potential cancer chemopreventive agent.
29437230	6	37	theme	cancer	1506:1511	arg1	agent					1529:1533	a potential cancer chemopreventive agent	1494:1533	a potential cancer chemopreventive agent	1494:1533	Overall, our results show that RSB lectin might exert immunological effects on mouse splenic cells and could thus be used as a potential cancer chemopreventive agent.
29437230	7	38	dep	APPLICATION	1546:1556	arg1	RSB					1583:1585	RSB	1583:1585	RSB	1583:1585	PRACTICAL APPLICATION Japanese red sword bean (RSB) is a tropical perennial legume consumed in many Asian countries.
29437230	7	38	dep	APPLICATION	1546:1556	arg1	bean					1577:1580	Japanese red sword bean	1558:1580	PRACTICAL APPLICATION Japanese red sword bean (RSB)	1536:1586	PRACTICAL APPLICATION Japanese red sword bean (RSB) is a tropical perennial legume consumed in many Asian countries.
29437230	2	39	theme	amino	360:364	arg1	analyses					382:389	partial amino acid sequencing analyses	352:389	partial amino acid sequencing analyses	352:389	RSB lectin was purified using maltamyl-Sepharose 4B and subjected to amino acid composition and partial amino acid sequencing analyses, and evaluated for blood and carbohydrate specificity, mitogenic activity, splenic natural killer (NK) cell activity, and its effect on B16 melanoma cell proliferation, compared with Concanavalin A (Con A).
29437230	0	40	theme	Cancer	82:87	arg1	Agent					105:109	a Potential Cancer Chemopreventive Agent	70:109	a Potential Cancer Chemopreventive Agent	70:109	Lectin Isolated from Japanese Red Sword Beans (Canavalia gladiata) as a Potential Cancer Chemopreventive Agent.
29437230	8	41	theme	rabbit	1796:1801	arg1	erythrocytes					1803:1814	mouse, sheep, and rabbit erythrocytes	1778:1814	mouse, sheep, and rabbit erythrocytes	1778:1814	RSB lectin shows specificity to mannose, glucose, maltose, methyl-d-mannoside, and thyroglobulin, but not to rhamnose, using mouse, sheep, and rabbit erythrocytes.
29437230	10	42	theme	bioactive	2160:2168	arg1	protein					2170:2176	a bioactive protein	2158:2176	a bioactive protein in medical research	2158:2196	Thus, RSB lectin has the potential to be used as a bioactive protein in medical research.
29437230	4	43	theme	Con	861:863	arg1	A					865:865	Con A	861:865	Con A	861:865	Compared with Con A, RSB lectin showed low resistance to proteases and to temperatures greater than 70 °C, but high mitogenic activity for mouse splenic cells.
29437230	8	44	theme	mouse	1778:1782	arg1	erythrocytes					1803:1814	mouse, sheep, and rabbit erythrocytes	1778:1814	mouse, sheep, and rabbit erythrocytes	1778:1814	RSB lectin shows specificity to mannose, glucose, maltose, methyl-d-mannoside, and thyroglobulin, but not to rhamnose, using mouse, sheep, and rabbit erythrocytes.
29437230	5	45	theme	RSB	1037:1039	arg1	lectin					1041:1046	RSB lectin	1037:1046	RSB lectin	1037:1046	Notably, while treatment with RSB lectin and Con A (0.01 and 0.1 μg/mL) promoted similar levels of splenic NK cell activity, which were higher than that observed in the control (0 μg/mL) and interleukin 2 (IL-2) (25 U)-treated populations, RBS lectin exerted a significantly stronger anti-proliferative effect than Con A at a concentration of 125.0 μg per well.
29437230	9	46	theme	splenic	2054:2060	arg1	cells					2082:2086	splenic natural killer (NK) cells	2054:2086	splenic natural killer (NK) cells	2054:2086	RSB lectin exhibits similarities to Concanavalin A in amino acid composition and sequence, shows mitogenic activity for mouse splenic cells and strong anti-proliferative activity for B16 melanoma cells, and also enhances the activity of splenic natural killer (NK) cells against YAC-1 cells.
29437230	8	47	theme	sheep	1785:1789	arg1	erythrocytes					1803:1814	mouse, sheep, and rabbit erythrocytes	1778:1814	mouse, sheep, and rabbit erythrocytes	1778:1814	RSB lectin shows specificity to mannose, glucose, maltose, methyl-d-mannoside, and thyroglobulin, but not to rhamnose, using mouse, sheep, and rabbit erythrocytes.
29437230	5	48	theme	125.0 μg	1350:1357	arg1	concentration					1333:1345	a concentration	1331:1345	a concentration of 125.0 μg per well	1331:1366	Notably, while treatment with RSB lectin and Con A (0.01 and 0.1 μg/mL) promoted similar levels of splenic NK cell activity, which were higher than that observed in the control (0 μg/mL) and interleukin 2 (IL-2) (25 U)-treated populations, RBS lectin exerted a significantly stronger anti-proliferative effect than Con A at a concentration of 125.0 μg per well.
29437230	9	49	theme	killer	2070:2075	arg1	cells					2082:2086	splenic natural killer (NK) cells	2054:2086	splenic natural killer (NK) cells	2054:2086	RSB lectin exhibits similarities to Concanavalin A in amino acid composition and sequence, shows mitogenic activity for mouse splenic cells and strong anti-proliferative activity for B16 melanoma cells, and also enhances the activity of splenic natural killer (NK) cells against YAC-1 cells.
29437230	2	50	theme	sequencing	371:380	arg1	analyses					382:389	partial amino acid sequencing analyses	352:389	partial amino acid sequencing analyses	352:389	RSB lectin was purified using maltamyl-Sepharose 4B and subjected to amino acid composition and partial amino acid sequencing analyses, and evaluated for blood and carbohydrate specificity, mitogenic activity, splenic natural killer (NK) cell activity, and its effect on B16 melanoma cell proliferation, compared with Concanavalin A (Con A).
29437230	0	51	theme	Red	30:32	arg1	Beans					40:44	Japanese Red Sword Beans	21:44	Japanese Red Sword Beans (Canavalia gladiata)	21:65	Lectin Isolated from Japanese Red Sword Beans (Canavalia gladiata) as a Potential Cancer Chemopreventive Agent.
29437230	0	51	theme	Red	30:32	arg1	gladiata					57:64	gladiata	57:64	gladiata	57:64	Lectin Isolated from Japanese Red Sword Beans (Canavalia gladiata) as a Potential Cancer Chemopreventive Agent.
29437230	10	52	theme	medical	2181:2187	arg1	research					2189:2196	medical research	2181:2196	medical research	2181:2196	Thus, RSB lectin has the potential to be used as a bioactive protein in medical research.
29437230	7	53	theme	many	1631:1634	arg1	countries					1642:1650	many Asian countries	1631:1650	many Asian countries	1631:1650	PRACTICAL APPLICATION Japanese red sword bean (RSB) is a tropical perennial legume consumed in many Asian countries.
29437230	2	54	theme	carbohydrate	420:431	arg1	specificity					433:443	carbohydrate specificity	420:443	carbohydrate specificity	420:443	RSB lectin was purified using maltamyl-Sepharose 4B and subjected to amino acid composition and partial amino acid sequencing analyses, and evaluated for blood and carbohydrate specificity, mitogenic activity, splenic natural killer (NK) cell activity, and its effect on B16 melanoma cell proliferation, compared with Concanavalin A (Con A).
29437230	5	55	theme	Con	1322:1324	arg1	A					1326:1326	Con A	1322:1326	Con A	1322:1326	Notably, while treatment with RSB lectin and Con A (0.01 and 0.1 μg/mL) promoted similar levels of splenic NK cell activity, which were higher than that observed in the control (0 μg/mL) and interleukin 2 (IL-2) (25 U)-treated populations, RBS lectin exerted a significantly stronger anti-proliferative effect than Con A at a concentration of 125.0 μg per well.
29437230	3	56	theme	rabbit	826:831	arg1	erythrocytes					833:844	mouse, sheep, and rabbit erythrocytes	808:844	erythrocytes	833:844	The amino acid composition and sequences of RSB lectin were similar to those of Con A. RSB lectin showed specificity to mannose, glucose, maltose, methyl-D-mannoside, and thyroglobulin, but not rhamnose, using mouse, sheep, and rabbit erythrocytes.
29437230	3	57	theme	amino	602:606	arg1	composition					613:623	amino acid composition	602:623	amino acid composition	602:623	The amino acid composition and sequences of RSB lectin were similar to those of Con A. RSB lectin showed specificity to mannose, glucose, maltose, methyl-D-mannoside, and thyroglobulin, but not rhamnose, using mouse, sheep, and rabbit erythrocytes.
29437230	2	58	theme	maltamyl-Sepharose	286:303	arg1	4B					305:306	maltamyl-Sepharose 4B	286:306	maltamyl-Sepharose 4B	286:306	RSB lectin was purified using maltamyl-Sepharose 4B and subjected to amino acid composition and partial amino acid sequencing analyses, and evaluated for blood and carbohydrate specificity, mitogenic activity, splenic natural killer (NK) cell activity, and its effect on B16 melanoma cell proliferation, compared with Concanavalin A (Con A).
29437230	4	59	theme	high	958:961	arg1	activity					973:980	high mitogenic activity	958:980	high mitogenic activity for mouse splenic cells	958:1004	Compared with Con A, RSB lectin showed low resistance to proteases and to temperatures greater than 70 °C, but high mitogenic activity for mouse splenic cells.
29437230	2	60	theme	acid	331:334	arg1	composition					336:346	amino acid composition	325:346	amino acid composition	325:346	RSB lectin was purified using maltamyl-Sepharose 4B and subjected to amino acid composition and partial amino acid sequencing analyses, and evaluated for blood and carbohydrate specificity, mitogenic activity, splenic natural killer (NK) cell activity, and its effect on B16 melanoma cell proliferation, compared with Concanavalin A (Con A).
29437230	1	61	theme	red	212:214	arg1	beans					222:226	Japanese red sword beans	203:226	Japanese red sword beans (Canavalia gladiata; RSBs)	203:253	In this study, we investigated the chemical and biological profile of lectin isolated from Japanese red sword beans (Canavalia gladiata; RSBs).
29437230	4	62	theme	low	886:888	arg1	resistance					890:899	low resistance	886:899	low resistance to proteases and to temperatures greater than 70 °C	886:951	Compared with Con A, RSB lectin showed low resistance to proteases and to temperatures greater than 70 °C, but high mitogenic activity for mouse splenic cells.
29437230	2	63	from	activity	499:506	arg1	proliferation					545:557	B16 melanoma cell proliferation	527:557	B16 melanoma cell proliferation	527:557	RSB lectin was purified using maltamyl-Sepharose 4B and subjected to amino acid composition and partial amino acid sequencing analyses, and evaluated for blood and carbohydrate specificity, mitogenic activity, splenic natural killer (NK) cell activity, and its effect on B16 melanoma cell proliferation, compared with Concanavalin A (Con A).
29437230	9	64	theme	strong	1961:1966	arg1	activity					1987:1994	strong anti-proliferative activity	1961:1994	strong anti-proliferative activity for B16 melanoma cells	1961:2017	RSB lectin exhibits similarities to Concanavalin A in amino acid composition and sequence, shows mitogenic activity for mouse splenic cells and strong anti-proliferative activity for B16 melanoma cells, and also enhances the activity of splenic natural killer (NK) cells against YAC-1 cells.
29437230	9	65	theme	acid	1877:1880	arg1	composition					1882:1892	amino acid composition	1871:1892	amino acid composition	1871:1892	RSB lectin exhibits similarities to Concanavalin A in amino acid composition and sequence, shows mitogenic activity for mouse splenic cells and strong anti-proliferative activity for B16 melanoma cells, and also enhances the activity of splenic natural killer (NK) cells against YAC-1 cells.
29437230	9	66	theme	mitogenic	1914:1922	arg1	activity					1924:1931	mitogenic activity	1914:1931	mitogenic activity for mouse splenic cells	1914:1955	RSB lectin exhibits similarities to Concanavalin A in amino acid composition and sequence, shows mitogenic activity for mouse splenic cells and strong anti-proliferative activity for B16 melanoma cells, and also enhances the activity of splenic natural killer (NK) cells against YAC-1 cells.
29437230	2	67	theme	cell	540:543	arg1	proliferation					545:557	B16 melanoma cell proliferation	527:557	B16 melanoma cell proliferation	527:557	RSB lectin was purified using maltamyl-Sepharose 4B and subjected to amino acid composition and partial amino acid sequencing analyses, and evaluated for blood and carbohydrate specificity, mitogenic activity, splenic natural killer (NK) cell activity, and its effect on B16 melanoma cell proliferation, compared with Concanavalin A (Con A).
29437230	1	68	theme	Canavalia	229:237	arg1	gladiata					239:246	Canavalia gladiata	229:246	Canavalia gladiata; RSBs	229:252	In this study, we investigated the chemical and biological profile of lectin isolated from Japanese red sword beans (Canavalia gladiata; RSBs).
29437230	2	69	theme	B16	527:529	arg1	proliferation					545:557	B16 melanoma cell proliferation	527:557	B16 melanoma cell proliferation	527:557	RSB lectin was purified using maltamyl-Sepharose 4B and subjected to amino acid composition and partial amino acid sequencing analyses, and evaluated for blood and carbohydrate specificity, mitogenic activity, splenic natural killer (NK) cell activity, and its effect on B16 melanoma cell proliferation, compared with Concanavalin A (Con A).
29437230	2	70	from	effect	517:522	arg1	proliferation					545:557	B16 melanoma cell proliferation	527:557	B16 melanoma cell proliferation	527:557	RSB lectin was purified using maltamyl-Sepharose 4B and subjected to amino acid composition and partial amino acid sequencing analyses, and evaluated for blood and carbohydrate specificity, mitogenic activity, splenic natural killer (NK) cell activity, and its effect on B16 melanoma cell proliferation, compared with Concanavalin A (Con A).
29437230	5	71	theme	RBS	1247:1249	arg1	lectin					1251:1256	RBS lectin	1247:1256	RBS lectin	1247:1256	Notably, while treatment with RSB lectin and Con A (0.01 and 0.1 μg/mL) promoted similar levels of splenic NK cell activity, which were higher than that observed in the control (0 μg/mL) and interleukin 2 (IL-2) (25 U)-treated populations, RBS lectin exerted a significantly stronger anti-proliferative effect than Con A at a concentration of 125.0 μg per well.
29437230	7	72	theme	sword	1571:1575	arg1	RSB					1583:1585	RSB	1583:1585	RSB	1583:1585	PRACTICAL APPLICATION Japanese red sword bean (RSB) is a tropical perennial legume consumed in many Asian countries.
29437230	7	72	theme	sword	1571:1575	arg1	bean					1577:1580	Japanese red sword bean	1558:1580	PRACTICAL APPLICATION Japanese red sword bean (RSB)	1536:1586	PRACTICAL APPLICATION Japanese red sword bean (RSB) is a tropical perennial legume consumed in many Asian countries.
29437230	3	73	theme	RSB	642:644	arg1	lectin					646:651	RSB lectin	642:651	RSB lectin	642:651	The amino acid composition and sequences of RSB lectin were similar to those of Con A. RSB lectin showed specificity to mannose, glucose, maltose, methyl-D-mannoside, and thyroglobulin, but not rhamnose, using mouse, sheep, and rabbit erythrocytes.
29437230	7	74	theme	Japanese	1558:1565	arg1	RSB					1583:1585	RSB	1583:1585	RSB	1583:1585	PRACTICAL APPLICATION Japanese red sword bean (RSB) is a tropical perennial legume consumed in many Asian countries.
29437230	7	74	theme	Japanese	1558:1565	arg1	bean					1577:1580	Japanese red sword bean	1558:1580	PRACTICAL APPLICATION Japanese red sword bean (RSB)	1536:1586	PRACTICAL APPLICATION Japanese red sword bean (RSB) is a tropical perennial legume consumed in many Asian countries.
29437230	2	75	from	activity	456:463	arg1	proliferation					545:557	B16 melanoma cell proliferation	527:557	B16 melanoma cell proliferation	527:557	RSB lectin was purified using maltamyl-Sepharose 4B and subjected to amino acid composition and partial amino acid sequencing analyses, and evaluated for blood and carbohydrate specificity, mitogenic activity, splenic natural killer (NK) cell activity, and its effect on B16 melanoma cell proliferation, compared with Concanavalin A (Con A).
29437230	4	76	theme	mouse	986:990	arg1	cells					1000:1004	mouse splenic cells	986:1004	mouse splenic cells	986:1004	Compared with Con A, RSB lectin showed low resistance to proteases and to temperatures greater than 70 °C, but high mitogenic activity for mouse splenic cells.
29437230	9	77	theme	splenic	1943:1949	arg1	cells					1951:1955	mouse splenic cells	1937:1955	mouse splenic cells	1937:1955	RSB lectin exhibits similarities to Concanavalin A in amino acid composition and sequence, shows mitogenic activity for mouse splenic cells and strong anti-proliferative activity for B16 melanoma cells, and also enhances the activity of splenic natural killer (NK) cells against YAC-1 cells.
29437230	1	78	theme	lectin	182:187	arg1	profile					171:177	the chemical and biological profile	143:177	the chemical and biological profile of lectin isolated from Japanese red sword beans (Canavalia gladiata; RSBs)	143:253	In this study, we investigated the chemical and biological profile of lectin isolated from Japanese red sword beans (Canavalia gladiata; RSBs).
29437230	9	79	theme	Concanavalin	1853:1864	arg1	A					1866:1866	Concanavalin A	1853:1866	Concanavalin A	1853:1866	RSB lectin exhibits similarities to Concanavalin A in amino acid composition and sequence, shows mitogenic activity for mouse splenic cells and strong anti-proliferative activity for B16 melanoma cells, and also enhances the activity of splenic natural killer (NK) cells against YAC-1 cells.
29437230	5	80	theme	cell	1117:1120	arg1	activity					1122:1129	splenic NK cell activity	1106:1129	splenic NK cell activity	1106:1129	Notably, while treatment with RSB lectin and Con A (0.01 and 0.1 μg/mL) promoted similar levels of splenic NK cell activity, which were higher than that observed in the control (0 μg/mL) and interleukin 2 (IL-2) (25 U)-treated populations, RBS lectin exerted a significantly stronger anti-proliferative effect than Con A at a concentration of 125.0 μg per well.
29437230	3	81	theme	A.	682:683	arg1	lectin					689:694	Con A. RSB lectin	678:694	Con A. RSB lectin showed specificity to mannose, glucose, maltose, methyl-D-mannoside, and thyroglobulin, but not rhamnose	678:799	The amino acid composition and sequences of RSB lectin were similar to those of Con A. RSB lectin showed specificity to mannose, glucose, maltose, methyl-D-mannoside, and thyroglobulin, but not rhamnose, using mouse, sheep, and rabbit erythrocytes.
29437230	1	82	dep	beans	222:226	arg1	gladiata					239:246	Canavalia gladiata	229:246	Canavalia gladiata; RSBs	229:252	In this study, we investigated the chemical and biological profile of lectin isolated from Japanese red sword beans (Canavalia gladiata; RSBs).
29437230	4	83	theme	mitogenic	963:971	arg1	activity					973:980	high mitogenic activity	958:980	high mitogenic activity for mouse splenic cells	958:1004	Compared with Con A, RSB lectin showed low resistance to proteases and to temperatures greater than 70 °C, but high mitogenic activity for mouse splenic cells.
29437230	9	84	theme	cells	2082:2086	arg1	activity					2042:2049	the activity	2038:2049	the activity of splenic natural killer (NK) cells against YAC-1 cells	2038:2106	RSB lectin exhibits similarities to Concanavalin A in amino acid composition and sequence, shows mitogenic activity for mouse splenic cells and strong anti-proliferative activity for B16 melanoma cells, and also enhances the activity of splenic natural killer (NK) cells against YAC-1 cells.
29437230	9	85	theme	melanoma	2004:2011	arg1	cells					2013:2017	B16 melanoma cells	2000:2017	B16 melanoma cells	2000:2017	RSB lectin exhibits similarities to Concanavalin A in amino acid composition and sequence, shows mitogenic activity for mouse splenic cells and strong anti-proliferative activity for B16 melanoma cells, and also enhances the activity of splenic natural killer (NK) cells against YAC-1 cells.
29437230	2	86	theme	mitogenic	446:454	arg1	activity					456:463	mitogenic activity	446:463	mitogenic activity	446:463	RSB lectin was purified using maltamyl-Sepharose 4B and subjected to amino acid composition and partial amino acid sequencing analyses, and evaluated for blood and carbohydrate specificity, mitogenic activity, splenic natural killer (NK) cell activity, and its effect on B16 melanoma cell proliferation, compared with Concanavalin A (Con A).
29437230	5	87	theme	anti-proliferative	1291:1308	arg1	effect					1310:1315	a significantly stronger anti-proliferative effect	1266:1315	a significantly stronger anti-proliferative effect than Con A	1266:1326	Notably, while treatment with RSB lectin and Con A (0.01 and 0.1 μg/mL) promoted similar levels of splenic NK cell activity, which were higher than that observed in the control (0 μg/mL) and interleukin 2 (IL-2) (25 U)-treated populations, RBS lectin exerted a significantly stronger anti-proliferative effect than Con A at a concentration of 125.0 μg per well.
29437230	5	88	theme	splenic	1106:1112	arg1	activity					1122:1129	splenic NK cell activity	1106:1129	splenic NK cell activity	1106:1129	Notably, while treatment with RSB lectin and Con A (0.01 and 0.1 μg/mL) promoted similar levels of splenic NK cell activity, which were higher than that observed in the control (0 μg/mL) and interleukin 2 (IL-2) (25 U)-treated populations, RBS lectin exerted a significantly stronger anti-proliferative effect than Con A at a concentration of 125.0 μg per well.
29437230	0	89	theme	Potential	72:80	arg1	Agent					105:109	a Potential Cancer Chemopreventive Agent	70:109	a Potential Cancer Chemopreventive Agent	70:109	Lectin Isolated from Japanese Red Sword Beans (Canavalia gladiata) as a Potential Cancer Chemopreventive Agent.
29437230	6	90	theme	chemopreventive	1513:1527	arg1	lectin					1404:1409	RSB lectin	1400:1409	RSB lectin	1400:1409	Overall, our results show that RSB lectin might exert immunological effects on mouse splenic cells and could thus be used as a potential cancer chemopreventive agent.
29437230	6	90	theme	chemopreventive	1513:1527	arg1	agent					1529:1533	a potential cancer chemopreventive agent	1494:1533	a potential cancer chemopreventive agent	1494:1533	Overall, our results show that RSB lectin might exert immunological effects on mouse splenic cells and could thus be used as a potential cancer chemopreventive agent.
29437230	0	91	theme	Chemopreventive	89:103	arg1	Agent					105:109	a Potential Cancer Chemopreventive Agent	70:109	a Potential Cancer Chemopreventive Agent	70:109	Lectin Isolated from Japanese Red Sword Beans (Canavalia gladiata) as a Potential Cancer Chemopreventive Agent.
29437230	6	92	theme	immunological	1423:1435	arg1	effects					1437:1443	immunological effects	1423:1443	immunological effects	1423:1443	Overall, our results show that RSB lectin might exert immunological effects on mouse splenic cells and could thus be used as a potential cancer chemopreventive agent.
29437230	3	93	theme	mouse	808:812	arg1	sheep					815:819	mouse, sheep, and rabbit erythrocytes	808:844	sheep	815:819	The amino acid composition and sequences of RSB lectin were similar to those of Con A. RSB lectin showed specificity to mannose, glucose, maltose, methyl-D-mannoside, and thyroglobulin, but not rhamnose, using mouse, sheep, and rabbit erythrocytes.
29437230	2	94	theme	natural	474:480	arg1	killer					482:487	splenic natural killer	466:487	splenic natural killer (NK) cell activity	466:506	RSB lectin was purified using maltamyl-Sepharose 4B and subjected to amino acid composition and partial amino acid sequencing analyses, and evaluated for blood and carbohydrate specificity, mitogenic activity, splenic natural killer (NK) cell activity, and its effect on B16 melanoma cell proliferation, compared with Concanavalin A (Con A).
29437230	2	94	theme	natural	474:480	arg1	NK					490:491	NK	490:491	NK	490:491	RSB lectin was purified using maltamyl-Sepharose 4B and subjected to amino acid composition and partial amino acid sequencing analyses, and evaluated for blood and carbohydrate specificity, mitogenic activity, splenic natural killer (NK) cell activity, and its effect on B16 melanoma cell proliferation, compared with Concanavalin A (Con A).
29437230	6	95	theme	potential	1496:1504	arg1	lectin					1404:1409	RSB lectin	1400:1409	RSB lectin	1400:1409	Overall, our results show that RSB lectin might exert immunological effects on mouse splenic cells and could thus be used as a potential cancer chemopreventive agent.
29437230	6	95	theme	potential	1496:1504	arg1	agent					1529:1533	a potential cancer chemopreventive agent	1494:1533	a potential cancer chemopreventive agent	1494:1533	Overall, our results show that RSB lectin might exert immunological effects on mouse splenic cells and could thus be used as a potential cancer chemopreventive agent.
29437230	2	96	theme	partial	352:358	arg1	analyses					382:389	partial amino acid sequencing analyses	352:389	partial amino acid sequencing analyses	352:389	RSB lectin was purified using maltamyl-Sepharose 4B and subjected to amino acid composition and partial amino acid sequencing analyses, and evaluated for blood and carbohydrate specificity, mitogenic activity, splenic natural killer (NK) cell activity, and its effect on B16 melanoma cell proliferation, compared with Concanavalin A (Con A).
29437230	10	97	from	protein	2170:2176	arg1	research					2189:2196	medical research	2181:2196	medical research	2181:2196	Thus, RSB lectin has the potential to be used as a bioactive protein in medical research.
29437230	0	98	theme	Japanese	21:28	arg1	Beans					40:44	Japanese Red Sword Beans	21:44	Japanese Red Sword Beans (Canavalia gladiata)	21:65	Lectin Isolated from Japanese Red Sword Beans (Canavalia gladiata) as a Potential Cancer Chemopreventive Agent.
29437230	0	98	theme	Japanese	21:28	arg1	gladiata					57:64	gladiata	57:64	gladiata	57:64	Lectin Isolated from Japanese Red Sword Beans (Canavalia gladiata) as a Potential Cancer Chemopreventive Agent.
29437230	3	99	dep	composition	613:623	arg1	The					598:600	The	598:600	The	598:600	The amino acid composition and sequences of RSB lectin were similar to those of Con A. RSB lectin showed specificity to mannose, glucose, maltose, methyl-D-mannoside, and thyroglobulin, but not rhamnose, using mouse, sheep, and rabbit erythrocytes.
29437230	6	100	theme	RSB	1400:1402	arg1	lectin					1404:1409	RSB lectin	1400:1409	RSB lectin	1400:1409	Overall, our results show that RSB lectin might exert immunological effects on mouse splenic cells and could thus be used as a potential cancer chemopreventive agent.
29437230	6	100	theme	RSB	1400:1402	arg1	agent					1529:1533	a potential cancer chemopreventive agent	1494:1533	a potential cancer chemopreventive agent	1494:1533	Overall, our results show that RSB lectin might exert immunological effects on mouse splenic cells and could thus be used as a potential cancer chemopreventive agent.
29437230	9	101	theme	RSB	1817:1819	arg1	lectin					1821:1826	RSB lectin	1817:1826	RSB lectin	1817:1826	RSB lectin exhibits similarities to Concanavalin A in amino acid composition and sequence, shows mitogenic activity for mouse splenic cells and strong anti-proliferative activity for B16 melanoma cells, and also enhances the activity of splenic natural killer (NK) cells against YAC-1 cells.
29437230	9	102	theme	YAC-1	2096:2100	arg1	cells					2102:2106	YAC-1 cells	2096:2106	YAC-1 cells	2096:2106	RSB lectin exhibits similarities to Concanavalin A in amino acid composition and sequence, shows mitogenic activity for mouse splenic cells and strong anti-proliferative activity for B16 melanoma cells, and also enhances the activity of splenic natural killer (NK) cells against YAC-1 cells.
29437230	0	103	theme	Sword	34:38	arg1	Beans					40:44	Japanese Red Sword Beans	21:44	Japanese Red Sword Beans (Canavalia gladiata)	21:65	Lectin Isolated from Japanese Red Sword Beans (Canavalia gladiata) as a Potential Cancer Chemopreventive Agent.
29437230	0	103	theme	Sword	34:38	arg1	gladiata					57:64	gladiata	57:64	gladiata	57:64	Lectin Isolated from Japanese Red Sword Beans (Canavalia gladiata) as a Potential Cancer Chemopreventive Agent.
29437230	5	104	theme	Con	1052:1054	arg1	A					1056:1056	Con A	1052:1056	Con A (0.01 and 0.1 μg/mL)	1052:1077	Notably, while treatment with RSB lectin and Con A (0.01 and 0.1 μg/mL) promoted similar levels of splenic NK cell activity, which were higher than that observed in the control (0 μg/mL) and interleukin 2 (IL-2) (25 U)-treated populations, RBS lectin exerted a significantly stronger anti-proliferative effect than Con A at a concentration of 125.0 μg per well.
29437230	4	105	theme	RSB	868:870	arg1	lectin					872:877	RSB lectin	868:877	RSB lectin	868:877	Compared with Con A, RSB lectin showed low resistance to proteases and to temperatures greater than 70 °C, but high mitogenic activity for mouse splenic cells.
29437230	2	106	theme	acid	366:369	arg1	analyses					382:389	partial amino acid sequencing analyses	352:389	partial amino acid sequencing analyses	352:389	RSB lectin was purified using maltamyl-Sepharose 4B and subjected to amino acid composition and partial amino acid sequencing analyses, and evaluated for blood and carbohydrate specificity, mitogenic activity, splenic natural killer (NK) cell activity, and its effect on B16 melanoma cell proliferation, compared with Concanavalin A (Con A).
29437230	7	107	theme	Asian	1636:1640	arg1	countries					1642:1650	many Asian countries	1631:1650	many Asian countries	1631:1650	PRACTICAL APPLICATION Japanese red sword bean (RSB) is a tropical perennial legume consumed in many Asian countries.
29437230	9	108	theme	natural	2062:2068	arg1	NK					2078:2079	NK	2078:2079	NK	2078:2079	RSB lectin exhibits similarities to Concanavalin A in amino acid composition and sequence, shows mitogenic activity for mouse splenic cells and strong anti-proliferative activity for B16 melanoma cells, and also enhances the activity of splenic natural killer (NK) cells against YAC-1 cells.
29437230	9	108	theme	natural	2062:2068	arg1	killer					2070:2075	natural killer	2062:2075	splenic natural killer (NK) cells	2054:2086	RSB lectin exhibits similarities to Concanavalin A in amino acid composition and sequence, shows mitogenic activity for mouse splenic cells and strong anti-proliferative activity for B16 melanoma cells, and also enhances the activity of splenic natural killer (NK) cells against YAC-1 cells.
29437230	1	109	attach	isolated	189:196	arg2	lectin					182:187	lectin	182:187	lectin isolated from Japanese red sword beans (Canavalia gladiata; RSBs)	182:253	In this study, we investigated the chemical and biological profile of lectin isolated from Japanese red sword beans (Canavalia gladiata; RSBs).
29437230	1	109	attach	isolated	189:196	arg1	beans					222:226	Japanese red sword beans	203:226	Japanese red sword beans (Canavalia gladiata; RSBs)	203:253	In this study, we investigated the chemical and biological profile of lectin isolated from Japanese red sword beans (Canavalia gladiata; RSBs).
29437230	1	110	theme	Japanese	203:210	arg1	beans					222:226	Japanese red sword beans	203:226	Japanese red sword beans (Canavalia gladiata; RSBs)	203:253	In this study, we investigated the chemical and biological profile of lectin isolated from Japanese red sword beans (Canavalia gladiata; RSBs).
29437230	9	111	theme	amino	1871:1875	arg1	composition					1882:1892	amino acid composition	1871:1892	amino acid composition	1871:1892	RSB lectin exhibits similarities to Concanavalin A in amino acid composition and sequence, shows mitogenic activity for mouse splenic cells and strong anti-proliferative activity for B16 melanoma cells, and also enhances the activity of splenic natural killer (NK) cells against YAC-1 cells.
29437230	1	112	theme	sword	216:220	arg1	beans					222:226	Japanese red sword beans	203:226	Japanese red sword beans (Canavalia gladiata; RSBs)	203:253	In this study, we investigated the chemical and biological profile of lectin isolated from Japanese red sword beans (Canavalia gladiata; RSBs).
29437230	2	113	theme	amino	325:329	arg1	composition					336:346	amino acid composition	325:346	amino acid composition	325:346	RSB lectin was purified using maltamyl-Sepharose 4B and subjected to amino acid composition and partial amino acid sequencing analyses, and evaluated for blood and carbohydrate specificity, mitogenic activity, splenic natural killer (NK) cell activity, and its effect on B16 melanoma cell proliferation, compared with Concanavalin A (Con A).
29437230	5	114	theme	similar	1088:1094	arg1	levels					1096:1101	similar levels	1088:1101	similar levels	1088:1101	Notably, while treatment with RSB lectin and Con A (0.01 and 0.1 μg/mL) promoted similar levels of splenic NK cell activity, which were higher than that observed in the control (0 μg/mL) and interleukin 2 (IL-2) (25 U)-treated populations, RBS lectin exerted a significantly stronger anti-proliferative effect than Con A at a concentration of 125.0 μg per well.
31139197	8	0	theme	screening	1297:1305	arg1	method					1307:1312	a complementary screening method	1281:1312	a complementary screening method directly targeting the presence and composition of relevant polysaccharides	1281:1388	The carbohydrate detecting microarrays analyses showed distinctive differences in the polysaccharide composition between the different species and carbohydrate detecting microarrays therefore has potential as a complementary screening method directly targeting the presence and composition of relevant polysaccharides.
31139197	8	0	theme	screening	1297:1305	arg1	potential					1268:1276	potential	1268:1276	potential	1268:1276	The carbohydrate detecting microarrays analyses showed distinctive differences in the polysaccharide composition between the different species and carbohydrate detecting microarrays therefore has potential as a complementary screening method directly targeting the presence and composition of relevant polysaccharides.
31139197	6	1	theme	study	715:719	arg1	aim					703:705	The aim	699:705	The aim of this study	699:719	The aim of this study was to use carbohydrate detecting microarrays to investigate the seasonal variation in the polysaccharide composition of two medicinal and two non-medicinal Aloe species over the course of a year.
31139197	4	2	theme	Aloe	561:564	arg1	products					566:573	Aloe products	561:573	Aloe products	561:573	The complexity of the polysaccharides has hindered development of relevant assays for authentication of Aloe products.
31139197	8	3	theme	polysaccharide	1158:1171	arg1	composition					1173:1183	the polysaccharide composition	1154:1183	the polysaccharide composition	1154:1183	The carbohydrate detecting microarrays analyses showed distinctive differences in the polysaccharide composition between the different species and carbohydrate detecting microarrays therefore has potential as a complementary screening method directly targeting the presence and composition of relevant polysaccharides.
31139197	4	4	theme	polysaccharides	479:493	arg1	complexity					461:470	The complexity	457:470	The complexity of the polysaccharides	457:493	The complexity of the polysaccharides has hindered development of relevant assays for authentication of Aloe products.
31139197	5	5	theme	Carbohydrate	576:587	arg1	method					645:650	a method	643:650	a method for profiling Aloe polysaccharide composition	643:696	Carbohydrate detecting microarrays have recently been suggested as a method for profiling Aloe polysaccharide composition.
31139197	5	5	theme	Carbohydrate	576:587	arg1	microarrays					599:609	Carbohydrate detecting microarrays	576:609	Carbohydrate detecting microarrays	576:609	Carbohydrate detecting microarrays have recently been suggested as a method for profiling Aloe polysaccharide composition.
31139197	7	6	from	cells	962:966	arg1	polysaccharides					982:996	the bioactive polysaccharides	968:996	the bioactive polysaccharides	968:996	Microscopy was used to explore where in the cells the bioactive polysaccharides are present and predict their functional role in the cell wall structure.
31139197	7	6	from	cells	962:966	arg1	present					1002:1008	present	1002:1008	present	1002:1008	Microscopy was used to explore where in the cells the bioactive polysaccharides are present and predict their functional role in the cell wall structure.
31139197	0	7	from	Detection	0:8	arg1	Polysaccharides					40:54	Aloe Polysaccharides	35:54	Aloe Polysaccharides	35:54	Detection of Seasonal Variation in Aloe Polysaccharides Using Carbohydrate Detecting Microarrays.
31139197	5	8	theme	detecting	589:597	arg1	method					645:650	a method	643:650	a method for profiling Aloe polysaccharide composition	643:696	Carbohydrate detecting microarrays have recently been suggested as a method for profiling Aloe polysaccharide composition.
31139197	5	8	theme	detecting	589:597	arg1	microarrays					599:609	Carbohydrate detecting microarrays	576:609	Carbohydrate detecting microarrays	576:609	Carbohydrate detecting microarrays have recently been suggested as a method for profiling Aloe polysaccharide composition.
31139197	8	9	dep	presence	1337:1344	arg1	the					1333:1335	the	1333:1335	the	1333:1335	The carbohydrate detecting microarrays analyses showed distinctive differences in the polysaccharide composition between the different species and carbohydrate detecting microarrays therefore has potential as a complementary screening method directly targeting the presence and composition of relevant polysaccharides.
31139197	7	10	theme	bioactive	972:980	arg1	polysaccharides					982:996	the bioactive polysaccharides	968:996	the bioactive polysaccharides	968:996	Microscopy was used to explore where in the cells the bioactive polysaccharides are present and predict their functional role in the cell wall structure.
31139197	7	10	theme	bioactive	972:980	arg1	present					1002:1008	present	1002:1008	present	1002:1008	Microscopy was used to explore where in the cells the bioactive polysaccharides are present and predict their functional role in the cell wall structure.
31139197	9	11	theme	polysaccharides	1615:1629	arg1	yield					1597:1601	higher yield	1590:1601	higher yield of relevant polysaccharides	1590:1629	The results also show changes in the polysaccharide composition over the year within the investigated species, which may be of importance for commercial growing in optimizing harvest times to obtain higher yield of relevant polysaccharides.
31139197	3	12	from	%	285:285	arg1	Aloe					319:322	the genus Aloe	309:322	the genus Aloe	309:322	Nearly 25% of the 600 species in the genus Aloe are used locally in traditional medicine, indicating that the bioactive components in Aloe vera may be common across the genus Aloe.
31139197	0	13	from	Polysaccharides	40:54	arg1	Detection					0:8	Detection	0:8	Detection of Seasonal Variation in Aloe Polysaccharides	0:54	Detection of Seasonal Variation in Aloe Polysaccharides Using Carbohydrate Detecting Microarrays.
31139197	3	14	used	used	328:331	arg2	species					298:304	the 600 species	290:304	the 600 species in the genus Aloe	290:322	Nearly 25% of the 600 species in the genus Aloe are used locally in traditional medicine, indicating that the bioactive components in Aloe vera may be common across the genus Aloe.
31139197	3	14	used	used	328:331	arg2	%					285:285	Nearly 25%	276:285	Nearly 25% of the 600 species in the genus Aloe	276:322	Nearly 25% of the 600 species in the genus Aloe are used locally in traditional medicine, indicating that the bioactive components in Aloe vera may be common across the genus Aloe.
31139197	8	15	theme	different	1197:1205	arg1	species					1207:1213	the different species	1193:1213	the different species	1193:1213	The carbohydrate detecting microarrays analyses showed distinctive differences in the polysaccharide composition between the different species and carbohydrate detecting microarrays therefore has potential as a complementary screening method directly targeting the presence and composition of relevant polysaccharides.
31139197	7	16	located	present	1002:1008	arg2	polysaccharides					982:996	the bioactive polysaccharides	968:996	the bioactive polysaccharides	968:996	Microscopy was used to explore where in the cells the bioactive polysaccharides are present and predict their functional role in the cell wall structure.
31139197	7	16	located	present	1002:1008	arg2	present					1002:1008	present	1002:1008	present	1002:1008	Microscopy was used to explore where in the cells the bioactive polysaccharides are present and predict their functional role in the cell wall structure.
31139197	7	16	located	present	1002:1008	arg1	cells					962:966	the cells	958:966	the cells	958:966	Microscopy was used to explore where in the cells the bioactive polysaccharides are present and predict their functional role in the cell wall structure.
31139197	8	17	theme	complementary	1283:1295	arg1	method					1307:1312	a complementary screening method	1281:1312	a complementary screening method directly targeting the presence and composition of relevant polysaccharides	1281:1388	The carbohydrate detecting microarrays analyses showed distinctive differences in the polysaccharide composition between the different species and carbohydrate detecting microarrays therefore has potential as a complementary screening method directly targeting the presence and composition of relevant polysaccharides.
31139197	8	17	theme	complementary	1283:1295	arg1	potential					1268:1276	potential	1268:1276	potential	1268:1276	The carbohydrate detecting microarrays analyses showed distinctive differences in the polysaccharide composition between the different species and carbohydrate detecting microarrays therefore has potential as a complementary screening method directly targeting the presence and composition of relevant polysaccharides.
31139197	4	18	theme	products	566:573	arg1	authentication					543:556	authentication	543:556	authentication of Aloe products	543:573	The complexity of the polysaccharides has hindered development of relevant assays for authentication of Aloe products.
31139197	0	19	theme	Aloe	35:38	arg1	Polysaccharides					40:54	Aloe Polysaccharides	35:54	Aloe Polysaccharides	35:54	Detection of Seasonal Variation in Aloe Polysaccharides Using Carbohydrate Detecting Microarrays.
31139197	9	20	from	changes	1413:1419	arg1	composition					1443:1453	the polysaccharide composition	1424:1453	the polysaccharide composition over the year within the investigated species, which may be of importance for commercial growing in optimizing harvest times to obtain higher yield of relevant polysaccharides	1424:1629	The results also show changes in the polysaccharide composition over the year within the investigated species, which may be of importance for commercial growing in optimizing harvest times to obtain higher yield of relevant polysaccharides.
31139197	7	21	from	role	1039:1042	arg1	structure					1061:1069	the cell wall structure	1047:1069	the cell wall structure	1047:1069	Microscopy was used to explore where in the cells the bioactive polysaccharides are present and predict their functional role in the cell wall structure.
31139197	8	22	theme	microarrays	1099:1109	arg1	analyses					1111:1118	The carbohydrate detecting microarrays analyses	1072:1118	The carbohydrate detecting microarrays analyses	1072:1118	The carbohydrate detecting microarrays analyses showed distinctive differences in the polysaccharide composition between the different species and carbohydrate detecting microarrays therefore has potential as a complementary screening method directly targeting the presence and composition of relevant polysaccharides.
31139197	8	23	theme	relevant	1365:1372	arg1	polysaccharides					1374:1388	relevant polysaccharides	1365:1388	relevant polysaccharides	1365:1388	The carbohydrate detecting microarrays analyses showed distinctive differences in the polysaccharide composition between the different species and carbohydrate detecting microarrays therefore has potential as a complementary screening method directly targeting the presence and composition of relevant polysaccharides.
31139197	3	24	theme	genus	313:317	arg1	Aloe					319:322	the genus Aloe	309:322	the genus Aloe	309:322	Nearly 25% of the 600 species in the genus Aloe are used locally in traditional medicine, indicating that the bioactive components in Aloe vera may be common across the genus Aloe.
31139197	1	25	theme	Aloe	98:101	arg1	gel					108:110	Aloe vera gel	98:110	Aloe vera gel	98:110	Aloe vera gel is a globally popular natural product used for the treatment of skin conditions.
31139197	1	25	theme	Aloe	98:101	arg1	product					142:148	a globally popular natural product	115:148	a globally popular natural product used for the treatment of skin conditions	115:190	Aloe vera gel is a globally popular natural product used for the treatment of skin conditions.
31139197	5	26	theme	polysaccharide	671:684	arg1	composition					686:696	Aloe polysaccharide composition	666:696	Aloe polysaccharide composition	666:696	Carbohydrate detecting microarrays have recently been suggested as a method for profiling Aloe polysaccharide composition.
31139197	1	27	theme	skin	176:179	arg1	conditions					181:190	skin conditions	176:190	skin conditions	176:190	Aloe vera gel is a globally popular natural product used for the treatment of skin conditions.
31139197	6	28	theme	year	912:915	arg1	course					900:905	the course	896:905	the course of a year	896:915	The aim of this study was to use carbohydrate detecting microarrays to investigate the seasonal variation in the polysaccharide composition of two medicinal and two non-medicinal Aloe species over the course of a year.
31139197	4	29	theme	assays	532:537	arg1	development					508:518	development	508:518	development of relevant assays for authentication of Aloe products	508:573	The complexity of the polysaccharides has hindered development of relevant assays for authentication of Aloe products.
31139197	1	30	theme	vera	103:106	arg1	gel					108:110	Aloe vera gel	98:110	Aloe vera gel	98:110	Aloe vera gel is a globally popular natural product used for the treatment of skin conditions.
31139197	1	30	theme	vera	103:106	arg1	product					142:148	a globally popular natural product	115:148	a globally popular natural product used for the treatment of skin conditions	115:190	Aloe vera gel is a globally popular natural product used for the treatment of skin conditions.
31139197	7	31	used	used	933:936	arg2	Microscopy					918:927	Microscopy	918:927	Microscopy	918:927	Microscopy was used to explore where in the cells the bioactive polysaccharides are present and predict their functional role in the cell wall structure.
31139197	3	32	theme	Aloe	410:413	arg1	vera					415:418	Aloe vera	410:418	Aloe vera	410:418	Nearly 25% of the 600 species in the genus Aloe are used locally in traditional medicine, indicating that the bioactive components in Aloe vera may be common across the genus Aloe.
31139197	0	33	theme	Variation	22:30	arg1	Detection					0:8	Detection	0:8	Detection of Seasonal Variation in Aloe Polysaccharides	0:54	Detection of Seasonal Variation in Aloe Polysaccharides Using Carbohydrate Detecting Microarrays.
31139197	4	34	theme	relevant	523:530	arg1	assays					532:537	relevant assays	523:537	relevant assays for authentication of Aloe products	523:573	The complexity of the polysaccharides has hindered development of relevant assays for authentication of Aloe products.
31139197	8	35	theme	carbohydrate	1076:1087	arg1	analyses					1111:1118	The carbohydrate detecting microarrays analyses	1072:1118	The carbohydrate detecting microarrays analyses	1072:1118	The carbohydrate detecting microarrays analyses showed distinctive differences in the polysaccharide composition between the different species and carbohydrate detecting microarrays therefore has potential as a complementary screening method directly targeting the presence and composition of relevant polysaccharides.
31139197	7	36	from	present	1002:1008	arg1	cells					962:966	the cells	958:966	the cells	958:966	Microscopy was used to explore where in the cells the bioactive polysaccharides are present and predict their functional role in the cell wall structure.
31139197	0	37	theme	Seasonal	13:20	arg1	Variation					22:30	Seasonal Variation	13:30	Seasonal Variation in Aloe Polysaccharides	13:54	Detection of Seasonal Variation in Aloe Polysaccharides Using Carbohydrate Detecting Microarrays.
31139197	6	38	theme	species	883:889	arg1	composition					827:837	the polysaccharide composition	808:837	the polysaccharide composition of two medicinal and two non-medicinal Aloe species	808:889	The aim of this study was to use carbohydrate detecting microarrays to investigate the seasonal variation in the polysaccharide composition of two medicinal and two non-medicinal Aloe species over the course of a year.
31139197	8	39	theme	detecting	1089:1097	arg1	analyses					1111:1118	The carbohydrate detecting microarrays analyses	1072:1118	The carbohydrate detecting microarrays analyses	1072:1118	The carbohydrate detecting microarrays analyses showed distinctive differences in the polysaccharide composition between the different species and carbohydrate detecting microarrays therefore has potential as a complementary screening method directly targeting the presence and composition of relevant polysaccharides.
31139197	3	40	theme	bioactive	386:394	arg1	common					427:432	common	427:432	common	427:432	Nearly 25% of the 600 species in the genus Aloe are used locally in traditional medicine, indicating that the bioactive components in Aloe vera may be common across the genus Aloe.
31139197	3	40	theme	bioactive	386:394	arg1	components					396:405	the bioactive components	382:405	the bioactive components in Aloe vera	382:418	Nearly 25% of the 600 species in the genus Aloe are used locally in traditional medicine, indicating that the bioactive components in Aloe vera may be common across the genus Aloe.
31139197	7	41	theme	cell	1051:1054	arg1	structure					1061:1069	the cell wall structure	1047:1069	the cell wall structure	1047:1069	Microscopy was used to explore where in the cells the bioactive polysaccharides are present and predict their functional role in the cell wall structure.
31139197	6	42	theme	Aloe	878:881	arg1	species					883:889	two medicinal and two non-medicinal Aloe species	842:889	species	883:889	The aim of this study was to use carbohydrate detecting microarrays to investigate the seasonal variation in the polysaccharide composition of two medicinal and two non-medicinal Aloe species over the course of a year.
31139197	1	43	theme	conditions	181:190	arg1	treatment					163:171	the treatment	159:171	the treatment of skin conditions	159:190	Aloe vera gel is a globally popular natural product used for the treatment of skin conditions.
31139197	6	44	from	variation	795:803	arg1	composition					827:837	the polysaccharide composition	808:837	the polysaccharide composition of two medicinal and two non-medicinal Aloe species	808:889	The aim of this study was to use carbohydrate detecting microarrays to investigate the seasonal variation in the polysaccharide composition of two medicinal and two non-medicinal Aloe species over the course of a year.
31139197	7	45	theme	wall	1056:1059	arg1	structure					1061:1069	the cell wall structure	1047:1069	the cell wall structure	1047:1069	Microscopy was used to explore where in the cells the bioactive polysaccharides are present and predict their functional role in the cell wall structure.
31139197	6	46	theme	non-medicinal	864:876	arg1	species					883:889	two medicinal and two non-medicinal Aloe species	842:889	species	883:889	The aim of this study was to use carbohydrate detecting microarrays to investigate the seasonal variation in the polysaccharide composition of two medicinal and two non-medicinal Aloe species over the course of a year.
31139197	3	47	theme	traditional	344:354	arg1	medicine					356:363	traditional medicine	344:363	traditional medicine	344:363	Nearly 25% of the 600 species in the genus Aloe are used locally in traditional medicine, indicating that the bioactive components in Aloe vera may be common across the genus Aloe.
31139197	9	48	theme	investigated	1480:1491	arg1	species					1493:1499	the investigated species	1476:1499	the investigated species	1476:1499	The results also show changes in the polysaccharide composition over the year within the investigated species, which may be of importance for commercial growing in optimizing harvest times to obtain higher yield of relevant polysaccharides.
31139197	7	49	attach	present	1002:1008	arg1	cells					962:966	the cells	958:966	the cells	958:966	Microscopy was used to explore where in the cells the bioactive polysaccharides are present and predict their functional role in the cell wall structure.
31139197	7	49	attach	present	1002:1008	arg2	present					1002:1008	present	1002:1008	present	1002:1008	Microscopy was used to explore where in the cells the bioactive polysaccharides are present and predict their functional role in the cell wall structure.
31139197	7	49	attach	present	1002:1008	arg2	polysaccharides					982:996	the bioactive polysaccharides	968:996	the bioactive polysaccharides	968:996	Microscopy was used to explore where in the cells the bioactive polysaccharides are present and predict their functional role in the cell wall structure.
31139197	9	50	theme	polysaccharide	1428:1441	arg1	composition					1443:1453	the polysaccharide composition	1424:1453	the polysaccharide composition over the year within the investigated species, which may be of importance for commercial growing in optimizing harvest times to obtain higher yield of relevant polysaccharides	1424:1629	The results also show changes in the polysaccharide composition over the year within the investigated species, which may be of importance for commercial growing in optimizing harvest times to obtain higher yield of relevant polysaccharides.
31139197	5	51	theme	Aloe	666:669	arg1	composition					686:696	Aloe polysaccharide composition	666:696	Aloe polysaccharide composition	666:696	Carbohydrate detecting microarrays have recently been suggested as a method for profiling Aloe polysaccharide composition.
31139197	9	52	theme	higher	1590:1595	arg1	yield					1597:1601	higher yield	1590:1601	higher yield of relevant polysaccharides	1590:1629	The results also show changes in the polysaccharide composition over the year within the investigated species, which may be of importance for commercial growing in optimizing harvest times to obtain higher yield of relevant polysaccharides.
31139197	3	53	from	components	396:405	arg1	vera					415:418	Aloe vera	410:418	Aloe vera	410:418	Nearly 25% of the 600 species in the genus Aloe are used locally in traditional medicine, indicating that the bioactive components in Aloe vera may be common across the genus Aloe.
31139197	3	54	theme	species	298:304	arg1	%					285:285	Nearly 25%	276:285	Nearly 25% of the 600 species in the genus Aloe	276:322	Nearly 25% of the 600 species in the genus Aloe are used locally in traditional medicine, indicating that the bioactive components in Aloe vera may be common across the genus Aloe.
31139197	3	54	theme	species	298:304	arg1	species					298:304	the 600 species	290:304	the 600 species in the genus Aloe	290:322	Nearly 25% of the 600 species in the genus Aloe are used locally in traditional medicine, indicating that the bioactive components in Aloe vera may be common across the genus Aloe.
31139197	9	55	theme	relevant	1606:1613	arg1	polysaccharides					1615:1629	relevant polysaccharides	1606:1629	relevant polysaccharides	1606:1629	The results also show changes in the polysaccharide composition over the year within the investigated species, which may be of importance for commercial growing in optimizing harvest times to obtain higher yield of relevant polysaccharides.
31139197	6	56	theme	medicinal	846:854	arg1	composition					827:837	the polysaccharide composition	808:837	the polysaccharide composition of two medicinal and two non-medicinal Aloe species	808:889	The aim of this study was to use carbohydrate detecting microarrays to investigate the seasonal variation in the polysaccharide composition of two medicinal and two non-medicinal Aloe species over the course of a year.
31139197	6	57	theme	detecting	745:753	arg1	microarrays					755:765	carbohydrate detecting microarrays	732:765	carbohydrate detecting microarrays	732:765	The aim of this study was to use carbohydrate detecting microarrays to investigate the seasonal variation in the polysaccharide composition of two medicinal and two non-medicinal Aloe species over the course of a year.
31139197	9	58	theme	harvest	1566:1572	arg1	times					1574:1578	harvest times	1566:1578	harvest times	1566:1578	The results also show changes in the polysaccharide composition over the year within the investigated species, which may be of importance for commercial growing in optimizing harvest times to obtain higher yield of relevant polysaccharides.
31139197	8	59	theme	carbohydrate	1219:1230	arg1	microarrays					1242:1252	carbohydrate detecting microarrays	1219:1252	carbohydrate detecting microarrays	1219:1252	The carbohydrate detecting microarrays analyses showed distinctive differences in the polysaccharide composition between the different species and carbohydrate detecting microarrays therefore has potential as a complementary screening method directly targeting the presence and composition of relevant polysaccharides.
31139197	7	60	theme	functional	1028:1037	arg1	role					1039:1042	their functional role	1022:1042	their functional role in the cell wall structure	1022:1069	Microscopy was used to explore where in the cells the bioactive polysaccharides are present and predict their functional role in the cell wall structure.
31139197	2	61	theme	useful	197:202	arg1	properties					204:213	Its useful properties	193:213	Its useful properties	193:213	Its useful properties are attributed to the presence of bioactive polysaccharides.
31139197	2	62	theme	polysaccharides	259:273	arg1	presence					237:244	the presence	233:244	the presence of bioactive polysaccharides	233:273	Its useful properties are attributed to the presence of bioactive polysaccharides.
31139197	6	63	theme	polysaccharide	812:825	arg1	composition					827:837	the polysaccharide composition	808:837	the polysaccharide composition of two medicinal and two non-medicinal Aloe species	808:889	The aim of this study was to use carbohydrate detecting microarrays to investigate the seasonal variation in the polysaccharide composition of two medicinal and two non-medicinal Aloe species over the course of a year.
31139197	1	64	theme	popular	126:132	arg1	gel					108:110	Aloe vera gel	98:110	Aloe vera gel	98:110	Aloe vera gel is a globally popular natural product used for the treatment of skin conditions.
31139197	1	64	theme	popular	126:132	arg1	product					142:148	a globally popular natural product	115:148	a globally popular natural product used for the treatment of skin conditions	115:190	Aloe vera gel is a globally popular natural product used for the treatment of skin conditions.
31139197	8	65	from	differences	1139:1149	arg1	composition					1173:1183	the polysaccharide composition	1154:1183	the polysaccharide composition	1154:1183	The carbohydrate detecting microarrays analyses showed distinctive differences in the polysaccharide composition between the different species and carbohydrate detecting microarrays therefore has potential as a complementary screening method directly targeting the presence and composition of relevant polysaccharides.
31139197	6	66	theme	carbohydrate	732:743	arg1	microarrays					755:765	carbohydrate detecting microarrays	732:765	carbohydrate detecting microarrays	732:765	The aim of this study was to use carbohydrate detecting microarrays to investigate the seasonal variation in the polysaccharide composition of two medicinal and two non-medicinal Aloe species over the course of a year.
31139197	2	67	theme	bioactive	249:257	arg1	polysaccharides					259:273	bioactive polysaccharides	249:273	bioactive polysaccharides	249:273	Its useful properties are attributed to the presence of bioactive polysaccharides.
31139197	1	68	theme	natural	134:140	arg1	gel					108:110	Aloe vera gel	98:110	Aloe vera gel	98:110	Aloe vera gel is a globally popular natural product used for the treatment of skin conditions.
31139197	1	68	theme	natural	134:140	arg1	product					142:148	a globally popular natural product	115:148	a globally popular natural product used for the treatment of skin conditions	115:190	Aloe vera gel is a globally popular natural product used for the treatment of skin conditions.
31139197	3	69	theme	genus	445:449	arg1	Aloe					451:454	the genus Aloe	441:454	the genus Aloe	441:454	Nearly 25% of the 600 species in the genus Aloe are used locally in traditional medicine, indicating that the bioactive components in Aloe vera may be common across the genus Aloe.
31139197	0	70	theme	Detecting	75:83	arg1	Microarrays					85:95	Carbohydrate Detecting Microarrays	62:95	Carbohydrate Detecting Microarrays	62:95	Detection of Seasonal Variation in Aloe Polysaccharides Using Carbohydrate Detecting Microarrays.
31139197	3	71	from	Aloe	319:322	arg1	%					285:285	Nearly 25%	276:285	Nearly 25% of the 600 species in the genus Aloe	276:322	Nearly 25% of the 600 species in the genus Aloe are used locally in traditional medicine, indicating that the bioactive components in Aloe vera may be common across the genus Aloe.
31139197	3	71	from	Aloe	319:322	arg1	species					298:304	the 600 species	290:304	the 600 species in the genus Aloe	290:322	Nearly 25% of the 600 species in the genus Aloe are used locally in traditional medicine, indicating that the bioactive components in Aloe vera may be common across the genus Aloe.
31139197	6	72	theme	seasonal	786:793	arg1	variation					795:803	the seasonal variation	782:803	the seasonal variation in the polysaccharide composition of two medicinal and two non-medicinal Aloe species	782:889	The aim of this study was to use carbohydrate detecting microarrays to investigate the seasonal variation in the polysaccharide composition of two medicinal and two non-medicinal Aloe species over the course of a year.
31139197	0	73	theme	Carbohydrate	62:73	arg1	Microarrays					85:95	Carbohydrate Detecting Microarrays	62:95	Carbohydrate Detecting Microarrays	62:95	Detection of Seasonal Variation in Aloe Polysaccharides Using Carbohydrate Detecting Microarrays.
31139197	0	74	from	Variation	22:30	arg1	Polysaccharides					40:54	Aloe Polysaccharides	35:54	Aloe Polysaccharides	35:54	Detection of Seasonal Variation in Aloe Polysaccharides Using Carbohydrate Detecting Microarrays.
31139197	8	75	theme	detecting	1232:1240	arg1	microarrays					1242:1252	carbohydrate detecting microarrays	1219:1252	carbohydrate detecting microarrays	1219:1252	The carbohydrate detecting microarrays analyses showed distinctive differences in the polysaccharide composition between the different species and carbohydrate detecting microarrays therefore has potential as a complementary screening method directly targeting the presence and composition of relevant polysaccharides.
31139197	8	76	theme	polysaccharides	1374:1388	arg1	composition					1350:1360	composition	1350:1360	composition	1350:1360	The carbohydrate detecting microarrays analyses showed distinctive differences in the polysaccharide composition between the different species and carbohydrate detecting microarrays therefore has potential as a complementary screening method directly targeting the presence and composition of relevant polysaccharides.
31139197	8	76	theme	polysaccharides	1374:1388	arg1	presence					1337:1344	presence	1337:1344	presence	1337:1344	The carbohydrate detecting microarrays analyses showed distinctive differences in the polysaccharide composition between the different species and carbohydrate detecting microarrays therefore has potential as a complementary screening method directly targeting the presence and composition of relevant polysaccharides.
31139197	8	77	theme	distinctive	1127:1137	arg1	differences					1139:1149	distinctive differences	1127:1149	distinctive differences in the polysaccharide composition between the different species and carbohydrate detecting microarrays	1127:1252	The carbohydrate detecting microarrays analyses showed distinctive differences in the polysaccharide composition between the different species and carbohydrate detecting microarrays therefore has potential as a complementary screening method directly targeting the presence and composition of relevant polysaccharides.
31139197	3	78	from	species	298:304	arg1	Aloe					319:322	the genus Aloe	309:322	the genus Aloe	309:322	Nearly 25% of the 600 species in the genus Aloe are used locally in traditional medicine, indicating that the bioactive components in Aloe vera may be common across the genus Aloe.
30761540	7	0	with	groups	1212:1217	arg1	n = 27					1225:1230	(n = 27)	1224:1231	(n = 27)	1224:1231	Patients were also divided into two groups with (n = 27) and without (n = 54) CT involvement, respectively.
30761540	2	1	theme	involvement	326:336	arg1	impact					291:296	the impact	287:296	the impact of cricothyroid (CT) muscle involvement on recovery	287:348	The present study examined the impact of cricothyroid (CT) muscle involvement on recovery using quantitative laryngeal electromyography (LEMG) of the thyroarytenoid-lateral cricoarytenoid (TA-LCA) muscle complex at multiple times postinjury.
30761540	1	2	theme	full	245:248	arg1	recovery					250:257	full recovery	245:257	full recovery	245:257	OBJECTIVES/HYPOTHESIS Wide variation in postinjury functional recovery is a hallmark of unilateral vocal fold paralysis (UVFP), ranging from zero to full recovery.
30761540	6	3	theme	P	1165:1165	arg1	assessment					1138:1147	the initial assessment	1126:1147	the initial assessment (300 ± 204 Hz) (P < .001)	1126:1173	The peak turn frequency of the TA-LCA muscle complex on the lesion side was improved at the follow-up (470 ± 294 Hz) compared with the initial assessment (300 ± 204 Hz) (P < .001).
30761540	6	3	theme	P	1165:1165	arg1	< .001					1167:1172	P < .001	1165:1172	P < .001	1165:1172	The peak turn frequency of the TA-LCA muscle complex on the lesion side was improved at the follow-up (470 ± 294 Hz) compared with the initial assessment (300 ± 204 Hz) (P < .001).
30761540	8	4	from	386 ± 265 Hz	1465:1476	arg1	involvement					1434:1444	CT involvement	1431:1444	CT involvement (from 345 ± 211 to 386 ± 265 Hz; P = .46)	1431:1486	TA-LCA muscle complex turn frequency improved in patients without CT involvement (from 277 ± 198 to 511 ± 301 Hz; P < .001), but not in those with CT involvement (from 345 ± 211 to 386 ± 265 Hz; P = .46).
30761540	3	5	theme	medical	545:551	arg1	center					553:558	a medical center	543:558	a medical center	543:558	STUDY DESIGN Prospective cohort study in a medical center.
30761540	9	6	theme	therapeutic	1601:1611	arg1	effects					1613:1619	similar therapeutic effects	1593:1619	similar therapeutic effects	1593:1619	Seventy-one of all patients received early intervention with intracordal hyaluronate injection, showing similar therapeutic effects in those with and without CT involvement.
30761540	11	7	theme	Early	1829:1833	arg1	interventions					1835:1847	Early interventions	1829:1847	Early interventions	1829:1847	Early interventions should be considered in patients with UVFP with CT involvement.
30761540	4	8	from	assessment	645:654	arg1	3					778:778	3	778:778	3	778:778	METHODS Eighty-one patients with UVFP (37 males and 44 females) received an initial assessment of quantitative LEMG, stroboscope, acoustic voice analysis and 36-Item Short Form Survey quality-of-life questionnaire at 3 to 6 months after UVFP onset and a follow-up assessment at 12 months after UVFP onset.
30761540	1	9	theme	unilateral	184:193	arg1	UVFP					217:220	UVFP	217:220	UVFP	217:220	OBJECTIVES/HYPOTHESIS Wide variation in postinjury functional recovery is a hallmark of unilateral vocal fold paralysis (UVFP), ranging from zero to full recovery.
30761540	1	9	theme	unilateral	184:193	arg1	paralysis					206:214	unilateral vocal fold paralysis	184:214	unilateral vocal fold paralysis (UVFP)	184:221	OBJECTIVES/HYPOTHESIS Wide variation in postinjury functional recovery is a hallmark of unilateral vocal fold paralysis (UVFP), ranging from zero to full recovery.
30761540	4	10	theme	quantitative	659:670	arg1	LEMG					672:675	quantitative LEMG	659:675	quantitative LEMG	659:675	METHODS Eighty-one patients with UVFP (37 males and 44 females) received an initial assessment of quantitative LEMG, stroboscope, acoustic voice analysis and 36-Item Short Form Survey quality-of-life questionnaire at 3 to 6 months after UVFP onset and a follow-up assessment at 12 months after UVFP onset.
30761540	8	11	from	511 ± 301 Hz	1384:1395	arg1	involvement					1353:1363	CT involvement	1350:1363	CT involvement (from 277 ± 198 to 511 ± 301 Hz; P < .001)	1350:1406	TA-LCA muscle complex turn frequency improved in patients without CT involvement (from 277 ± 198 to 511 ± 301 Hz; P < .001), but not in those with CT involvement (from 345 ± 211 to 386 ± 265 Hz; P = .46).
30761540	1	12	theme	fold	201:204	arg1	UVFP					217:220	UVFP	217:220	UVFP	217:220	OBJECTIVES/HYPOTHESIS Wide variation in postinjury functional recovery is a hallmark of unilateral vocal fold paralysis (UVFP), ranging from zero to full recovery.
30761540	1	12	theme	fold	201:204	arg1	paralysis					206:214	unilateral vocal fold paralysis	184:214	unilateral vocal fold paralysis (UVFP)	184:221	OBJECTIVES/HYPOTHESIS Wide variation in postinjury functional recovery is a hallmark of unilateral vocal fold paralysis (UVFP), ranging from zero to full recovery.
30761540	12	13	theme	EVIDENCE	1922:1929	arg1	LEVEL					1913:1917	LEVEL	1913:1917	LEVEL OF EVIDENCE	1913:1929	LEVEL OF EVIDENCE 2 Laryngoscope, 130:139-145, 2020.
30761540	12	13	theme	EVIDENCE	1922:1929	arg1	130:139-145					1947:1957	130:139-145	1947:1957	130:139-145	1947:1957	LEVEL OF EVIDENCE 2 Laryngoscope, 130:139-145, 2020.
30761540	12	13	theme	EVIDENCE	1922:1929	arg1	2020					1960:1963	2020	1960:1963	2020	1960:1963	LEVEL OF EVIDENCE 2 Laryngoscope, 130:139-145, 2020.
30761540	6	14	theme	initial	1130:1136	arg1	assessment					1138:1147	the initial assessment	1126:1147	the initial assessment (300 ± 204 Hz) (P < .001)	1126:1173	The peak turn frequency of the TA-LCA muscle complex on the lesion side was improved at the follow-up (470 ± 294 Hz) compared with the initial assessment (300 ± 204 Hz) (P < .001).
30761540	6	14	theme	initial	1130:1136	arg1	< .001					1167:1172	P < .001	1165:1172	P < .001	1165:1172	The peak turn frequency of the TA-LCA muscle complex on the lesion side was improved at the follow-up (470 ± 294 Hz) compared with the initial assessment (300 ± 204 Hz) (P < .001).
30761540	6	14	theme	initial	1130:1136	arg1	300 ± 204 Hz					1150:1161	300 ± 204 Hz	1150:1161	300 ± 204 Hz	1150:1161	The peak turn frequency of the TA-LCA muscle complex on the lesion side was improved at the follow-up (470 ± 294 Hz) compared with the initial assessment (300 ± 204 Hz) (P < .001).
30761540	3	15	theme	DESIGN	508:513	arg1	cohort					527:532	STUDY DESIGN Prospective cohort	502:532	STUDY DESIGN Prospective cohort	502:532	STUDY DESIGN Prospective cohort study in a medical center.
30761540	10	16	contain	has	1748:1750	arg1	UVFP					1681:1684	CONCLUSIONS Acute UVFP	1663:1684	CONCLUSIONS Acute UVFP with combined TA-LCA muscle complex and CT muscle involvement	1663:1746	CONCLUSIONS Acute UVFP with combined TA-LCA muscle complex and CT muscle involvement has a poor prognosis, with poorer recovery of TA-LCA muscle complex recruitment.
30761540	10	16	contain	has	1748:1750	arg2	prognosis					1759:1767	a poor prognosis	1752:1767	a poor prognosis	1752:1767	CONCLUSIONS Acute UVFP with combined TA-LCA muscle complex and CT muscle involvement has a poor prognosis, with poorer recovery of TA-LCA muscle complex recruitment.
30761540	4	17	theme	voice	700:704	arg1	analysis					706:713	acoustic voice analysis	691:713	acoustic voice analysis	691:713	METHODS Eighty-one patients with UVFP (37 males and 44 females) received an initial assessment of quantitative LEMG, stroboscope, acoustic voice analysis and 36-Item Short Form Survey quality-of-life questionnaire at 3 to 6 months after UVFP onset and a follow-up assessment at 12 months after UVFP onset.
30761540	10	18	theme	muscle	1729:1734	arg1	involvement					1736:1746	combined TA-LCA muscle complex and CT muscle involvement	1691:1746	involvement	1736:1746	CONCLUSIONS Acute UVFP with combined TA-LCA muscle complex and CT muscle involvement has a poor prognosis, with poorer recovery of TA-LCA muscle complex recruitment.
30761540	2	19	dep	cricothyroid	301:312	arg1	CT					315:316	CT	315:316	CT	315:316	The present study examined the impact of cricothyroid (CT) muscle involvement on recovery using quantitative laryngeal electromyography (LEMG) of the thyroarytenoid-lateral cricoarytenoid (TA-LCA) muscle complex at multiple times postinjury.
30761540	1	20	theme	functional	147:156	arg1	recovery					158:165	postinjury functional recovery	136:165	postinjury functional recovery	136:165	OBJECTIVES/HYPOTHESIS Wide variation in postinjury functional recovery is a hallmark of unilateral vocal fold paralysis (UVFP), ranging from zero to full recovery.
30761540	10	21	with	UVFP	1681:1684	arg1	complex					1714:1720	combined TA-LCA muscle complex and CT muscle involvement	1691:1746	complex	1714:1720	CONCLUSIONS Acute UVFP with combined TA-LCA muscle complex and CT muscle involvement has a poor prognosis, with poorer recovery of TA-LCA muscle complex recruitment.
30761540	10	21	with	UVFP	1681:1684	arg1	involvement					1736:1746	combined TA-LCA muscle complex and CT muscle involvement	1691:1746	involvement	1736:1746	CONCLUSIONS Acute UVFP with combined TA-LCA muscle complex and CT muscle involvement has a poor prognosis, with poorer recovery of TA-LCA muscle complex recruitment.
30761540	4	22	with	patients	580:587	arg1	UVFP					594:597	UVFP	594:597	UVFP	594:597	METHODS Eighty-one patients with UVFP (37 males and 44 females) received an initial assessment of quantitative LEMG, stroboscope, acoustic voice analysis and 36-Item Short Form Survey quality-of-life questionnaire at 3 to 6 months after UVFP onset and a follow-up assessment at 12 months after UVFP onset.
30761540	2	23	from	impact	291:296	arg1	recovery					341:348	recovery	341:348	recovery	341:348	The present study examined the impact of cricothyroid (CT) muscle involvement on recovery using quantitative laryngeal electromyography (LEMG) of the thyroarytenoid-lateral cricoarytenoid (TA-LCA) muscle complex at multiple times postinjury.
30761540	0	24	theme	vocal	74:78	arg1	paralysis					85:93	unilateral vocal fold paralysis	63:93	unilateral vocal fold paralysis	63:93	The impact of cricothyroid involvement on adductor recovery in unilateral vocal fold paralysis.
30761540	4	25	from	3	778:778	arg1	assessment					825:834	a follow-up assessment	813:834	a follow-up assessment at 12 months after UVFP onset	813:864	METHODS Eighty-one patients with UVFP (37 males and 44 females) received an initial assessment of quantitative LEMG, stroboscope, acoustic voice analysis and 36-Item Short Form Survey quality-of-life questionnaire at 3 to 6 months after UVFP onset and a follow-up assessment at 12 months after UVFP onset.
30761540	4	25	from	3	778:778	arg1	analysis					706:713	acoustic voice analysis	691:713	acoustic voice analysis	691:713	METHODS Eighty-one patients with UVFP (37 males and 44 females) received an initial assessment of quantitative LEMG, stroboscope, acoustic voice analysis and 36-Item Short Form Survey quality-of-life questionnaire at 3 to 6 months after UVFP onset and a follow-up assessment at 12 months after UVFP onset.
30761540	4	25	from	3	778:778	arg1	questionnaire					761:773	36-Item Short Form Survey quality-of-life questionnaire	719:773	36-Item Short Form Survey quality-of-life questionnaire at 3 to 6 months after UVFP onset	719:807	METHODS Eighty-one patients with UVFP (37 males and 44 females) received an initial assessment of quantitative LEMG, stroboscope, acoustic voice analysis and 36-Item Short Form Survey quality-of-life questionnaire at 3 to 6 months after UVFP onset and a follow-up assessment at 12 months after UVFP onset.
30761540	4	25	from	3	778:778	arg1	LEMG					672:675	quantitative LEMG	659:675	quantitative LEMG	659:675	METHODS Eighty-one patients with UVFP (37 males and 44 females) received an initial assessment of quantitative LEMG, stroboscope, acoustic voice analysis and 36-Item Short Form Survey quality-of-life questionnaire at 3 to 6 months after UVFP onset and a follow-up assessment at 12 months after UVFP onset.
30761540	4	25	from	3	778:778	arg1	stroboscope					678:688	stroboscope	678:688	stroboscope	678:688	METHODS Eighty-one patients with UVFP (37 males and 44 females) received an initial assessment of quantitative LEMG, stroboscope, acoustic voice analysis and 36-Item Short Form Survey quality-of-life questionnaire at 3 to 6 months after UVFP onset and a follow-up assessment at 12 months after UVFP onset.
30761540	4	25	from	3	778:778	arg1	assessment					645:654	an initial assessment	634:654	an initial assessment of quantitative LEMG, stroboscope, acoustic voice analysis and 36-Item Short Form Survey quality-of-life questionnaire at 3 to 6 months after UVFP onset	634:807	METHODS Eighty-one patients with UVFP (37 males and 44 females) received an initial assessment of quantitative LEMG, stroboscope, acoustic voice analysis and 36-Item Short Form Survey quality-of-life questionnaire at 3 to 6 months after UVFP onset and a follow-up assessment at 12 months after UVFP onset.
30761540	5	26	theme	follow-up	891:899	arg1	assessments					901:911	follow-up assessments	891:911	follow-up assessments	891:911	RESULTS The initial and follow-up assessments were performed at 4.3 ± 1.9 and 12.5 ± 1.3 months after UVFP onset, respectively.
30761540	6	27	theme	peak	999:1002	arg1	frequency					1009:1017	The peak turn frequency	995:1017	The peak turn frequency of the TA-LCA muscle complex on the lesion side	995:1065	The peak turn frequency of the TA-LCA muscle complex on the lesion side was improved at the follow-up (470 ± 294 Hz) compared with the initial assessment (300 ± 204 Hz) (P < .001).
30761540	8	28	theme	CT	1431:1432	arg1	involvement					1434:1444	CT involvement	1431:1444	CT involvement (from 345 ± 211 to 386 ± 265 Hz; P = .46)	1431:1486	TA-LCA muscle complex turn frequency improved in patients without CT involvement (from 277 ± 198 to 511 ± 301 Hz; P < .001), but not in those with CT involvement (from 345 ± 211 to 386 ± 265 Hz; P = .46).
30761540	4	29	theme	questionnaire	761:773	arg1	assessment					645:654	an initial assessment	634:654	an initial assessment of quantitative LEMG, stroboscope, acoustic voice analysis and 36-Item Short Form Survey quality-of-life questionnaire at 3 to 6 months after UVFP onset	634:807	METHODS Eighty-one patients with UVFP (37 males and 44 females) received an initial assessment of quantitative LEMG, stroboscope, acoustic voice analysis and 36-Item Short Form Survey quality-of-life questionnaire at 3 to 6 months after UVFP onset and a follow-up assessment at 12 months after UVFP onset.
30761540	4	29	theme	questionnaire	761:773	arg1	assessment					825:834	a follow-up assessment	813:834	a follow-up assessment at 12 months after UVFP onset	813:864	METHODS Eighty-one patients with UVFP (37 males and 44 females) received an initial assessment of quantitative LEMG, stroboscope, acoustic voice analysis and 36-Item Short Form Survey quality-of-life questionnaire at 3 to 6 months after UVFP onset and a follow-up assessment at 12 months after UVFP onset.
30761540	10	30	theme	combined	1691:1698	arg1	complex					1714:1720	combined TA-LCA muscle complex and CT muscle involvement	1691:1746	complex	1714:1720	CONCLUSIONS Acute UVFP with combined TA-LCA muscle complex and CT muscle involvement has a poor prognosis, with poorer recovery of TA-LCA muscle complex recruitment.
30761540	8	31	theme	turn	1306:1309	arg1	frequency					1311:1319	TA-LCA muscle complex turn frequency	1284:1319	TA-LCA muscle complex turn frequency	1284:1319	TA-LCA muscle complex turn frequency improved in patients without CT involvement (from 277 ± 198 to 511 ± 301 Hz; P < .001), but not in those with CT involvement (from 345 ± 211 to 386 ± 265 Hz; P = .46).
30761540	2	32	theme	TA-LCA	449:454	arg1	complex					464:470	the thyroarytenoid-lateral cricoarytenoid (TA-LCA) muscle complex	406:470	the thyroarytenoid-lateral cricoarytenoid (TA-LCA) muscle complex	406:470	The present study examined the impact of cricothyroid (CT) muscle involvement on recovery using quantitative laryngeal electromyography (LEMG) of the thyroarytenoid-lateral cricoarytenoid (TA-LCA) muscle complex at multiple times postinjury.
30761540	12	33	dep	LEVEL	1913:1917	arg1	Laryngoscope					1933:1944	2 Laryngoscope	1931:1944	2 Laryngoscope	1931:1944	LEVEL OF EVIDENCE 2 Laryngoscope, 130:139-145, 2020.
30761540	4	34	theme	Short	727:731	arg1	questionnaire					761:773	36-Item Short Form Survey quality-of-life questionnaire	719:773	36-Item Short Form Survey quality-of-life questionnaire at 3 to 6 months after UVFP onset	719:807	METHODS Eighty-one patients with UVFP (37 males and 44 females) received an initial assessment of quantitative LEMG, stroboscope, acoustic voice analysis and 36-Item Short Form Survey quality-of-life questionnaire at 3 to 6 months after UVFP onset and a follow-up assessment at 12 months after UVFP onset.
30761540	2	35	theme	laryngeal	369:377	arg1	electromyography					379:394	quantitative laryngeal electromyography	356:394	quantitative laryngeal electromyography (LEMG) of the thyroarytenoid-lateral cricoarytenoid (TA-LCA) muscle complex	356:470	The present study examined the impact of cricothyroid (CT) muscle involvement on recovery using quantitative laryngeal electromyography (LEMG) of the thyroarytenoid-lateral cricoarytenoid (TA-LCA) muscle complex at multiple times postinjury.
30761540	2	35	theme	laryngeal	369:377	arg1	LEMG					397:400	LEMG	397:400	LEMG	397:400	The present study examined the impact of cricothyroid (CT) muscle involvement on recovery using quantitative laryngeal electromyography (LEMG) of the thyroarytenoid-lateral cricoarytenoid (TA-LCA) muscle complex at multiple times postinjury.
30761540	1	36	from	variation	123:131	arg1	recovery					158:165	postinjury functional recovery	136:165	postinjury functional recovery	136:165	OBJECTIVES/HYPOTHESIS Wide variation in postinjury functional recovery is a hallmark of unilateral vocal fold paralysis (UVFP), ranging from zero to full recovery.
30761540	6	37	theme	lesion	1055:1060	arg1	side					1062:1065	the lesion side	1051:1065	the lesion side	1051:1065	The peak turn frequency of the TA-LCA muscle complex on the lesion side was improved at the follow-up (470 ± 294 Hz) compared with the initial assessment (300 ± 204 Hz) (P < .001).
30761540	2	38	theme	cricoarytenoid	433:446	arg1	complex					464:470	the thyroarytenoid-lateral cricoarytenoid (TA-LCA) muscle complex	406:470	the thyroarytenoid-lateral cricoarytenoid (TA-LCA) muscle complex	406:470	The present study examined the impact of cricothyroid (CT) muscle involvement on recovery using quantitative laryngeal electromyography (LEMG) of the thyroarytenoid-lateral cricoarytenoid (TA-LCA) muscle complex at multiple times postinjury.
30761540	4	39	theme	Survey	738:743	arg1	questionnaire					761:773	36-Item Short Form Survey quality-of-life questionnaire	719:773	36-Item Short Form Survey quality-of-life questionnaire at 3 to 6 months after UVFP onset	719:807	METHODS Eighty-one patients with UVFP (37 males and 44 females) received an initial assessment of quantitative LEMG, stroboscope, acoustic voice analysis and 36-Item Short Form Survey quality-of-life questionnaire at 3 to 6 months after UVFP onset and a follow-up assessment at 12 months after UVFP onset.
30761540	1	40	theme	Wide	118:121	arg1	variation					123:131	OBJECTIVES/HYPOTHESIS Wide variation	96:131	OBJECTIVES/HYPOTHESIS Wide variation in postinjury functional recovery	96:165	OBJECTIVES/HYPOTHESIS Wide variation in postinjury functional recovery is a hallmark of unilateral vocal fold paralysis (UVFP), ranging from zero to full recovery.
30761540	1	40	theme	Wide	118:121	arg1	hallmark					172:179	a hallmark	170:179	a hallmark	170:179	OBJECTIVES/HYPOTHESIS Wide variation in postinjury functional recovery is a hallmark of unilateral vocal fold paralysis (UVFP), ranging from zero to full recovery.
30761540	7	41	theme	CT	1254:1255	arg1	involvement					1257:1267	(n = 54) CT involvement	1245:1267	(n = 54) CT involvement	1245:1267	Patients were also divided into two groups with (n = 27) and without (n = 54) CT involvement, respectively.
30761540	4	42	theme	METHODS	561:567	arg1	patients					580:587	METHODS Eighty-one patients	561:587	METHODS Eighty-one patients with UVFP (37 males and 44 females)	561:623	METHODS Eighty-one patients with UVFP (37 males and 44 females) received an initial assessment of quantitative LEMG, stroboscope, acoustic voice analysis and 36-Item Short Form Survey quality-of-life questionnaire at 3 to 6 months after UVFP onset and a follow-up assessment at 12 months after UVFP onset.
30761540	8	43	theme	CT	1350:1351	arg1	involvement					1353:1363	CT involvement	1350:1363	CT involvement (from 277 ± 198 to 511 ± 301 Hz; P < .001)	1350:1406	TA-LCA muscle complex turn frequency improved in patients without CT involvement (from 277 ± 198 to 511 ± 301 Hz; P < .001), but not in those with CT involvement (from 345 ± 211 to 386 ± 265 Hz; P = .46).
30761540	0	44	theme	involvement	27:37	arg1	impact					4:9	The impact	0:9	The impact of cricothyroid involvement on adductor recovery in unilateral vocal fold paralysis	0:93	The impact of cricothyroid involvement on adductor recovery in unilateral vocal fold paralysis.
30761540	9	45	theme	patients	1508:1515	arg1	Seventy-one					1489:1499	Seventy-one	1489:1499	Seventy-one of all patients	1489:1515	Seventy-one of all patients received early intervention with intracordal hyaluronate injection, showing similar therapeutic effects in those with and without CT involvement.
30761540	6	46	theme	muscle	1033:1038	arg1	complex					1040:1046	the TA-LCA muscle complex	1022:1046	the TA-LCA muscle complex	1022:1046	The peak turn frequency of the TA-LCA muscle complex on the lesion side was improved at the follow-up (470 ± 294 Hz) compared with the initial assessment (300 ± 204 Hz) (P < .001).
30761540	2	47	theme	muscle	457:462	arg1	complex					464:470	the thyroarytenoid-lateral cricoarytenoid (TA-LCA) muscle complex	406:470	the thyroarytenoid-lateral cricoarytenoid (TA-LCA) muscle complex	406:470	The present study examined the impact of cricothyroid (CT) muscle involvement on recovery using quantitative laryngeal electromyography (LEMG) of the thyroarytenoid-lateral cricoarytenoid (TA-LCA) muscle complex at multiple times postinjury.
30761540	10	48	theme	poorer	1775:1780	arg1	recovery					1782:1789	poorer recovery	1775:1789	poorer recovery of TA-LCA muscle complex recruitment	1775:1826	CONCLUSIONS Acute UVFP with combined TA-LCA muscle complex and CT muscle involvement has a poor prognosis, with poorer recovery of TA-LCA muscle complex recruitment.
30761540	4	49	theme	follow-up	815:823	arg1	assessment					825:834	a follow-up assessment	813:834	a follow-up assessment at 12 months after UVFP onset	813:864	METHODS Eighty-one patients with UVFP (37 males and 44 females) received an initial assessment of quantitative LEMG, stroboscope, acoustic voice analysis and 36-Item Short Form Survey quality-of-life questionnaire at 3 to 6 months after UVFP onset and a follow-up assessment at 12 months after UVFP onset.
30761540	2	50	theme	muscle	319:324	arg1	involvement					326:336	cricothyroid (CT) muscle involvement	301:336	cricothyroid (CT) muscle involvement	301:336	The present study examined the impact of cricothyroid (CT) muscle involvement on recovery using quantitative laryngeal electromyography (LEMG) of the thyroarytenoid-lateral cricoarytenoid (TA-LCA) muscle complex at multiple times postinjury.
30761540	0	51	theme	adductor	42:49	arg1	recovery					51:58	adductor recovery	42:58	adductor recovery in unilateral vocal fold paralysis	42:93	The impact of cricothyroid involvement on adductor recovery in unilateral vocal fold paralysis.
30761540	9	52	theme	early	1526:1530	arg1	intervention					1532:1543	early intervention	1526:1543	early intervention	1526:1543	Seventy-one of all patients received early intervention with intracordal hyaluronate injection, showing similar therapeutic effects in those with and without CT involvement.
30761540	10	53	theme	poor	1754:1757	arg1	prognosis					1759:1767	a poor prognosis	1752:1767	a poor prognosis	1752:1767	CONCLUSIONS Acute UVFP with combined TA-LCA muscle complex and CT muscle involvement has a poor prognosis, with poorer recovery of TA-LCA muscle complex recruitment.
30761540	10	54	theme	recruitment	1816:1826	arg1	recovery					1782:1789	poorer recovery	1775:1789	poorer recovery of TA-LCA muscle complex recruitment	1775:1826	CONCLUSIONS Acute UVFP with combined TA-LCA muscle complex and CT muscle involvement has a poor prognosis, with poorer recovery of TA-LCA muscle complex recruitment.
30761540	10	55	theme	muscle	1801:1806	arg1	recruitment					1816:1826	TA-LCA muscle complex recruitment	1794:1826	TA-LCA muscle complex recruitment	1794:1826	CONCLUSIONS Acute UVFP with combined TA-LCA muscle complex and CT muscle involvement has a poor prognosis, with poorer recovery of TA-LCA muscle complex recruitment.
30761540	4	56	theme	UVFP	798:801	arg1	onset					803:807	UVFP onset	798:807	UVFP onset	798:807	METHODS Eighty-one patients with UVFP (37 males and 44 females) received an initial assessment of quantitative LEMG, stroboscope, acoustic voice analysis and 36-Item Short Form Survey quality-of-life questionnaire at 3 to 6 months after UVFP onset and a follow-up assessment at 12 months after UVFP onset.
30761540	3	57	theme	Prospective	515:525	arg1	cohort					527:532	STUDY DESIGN Prospective cohort	502:532	STUDY DESIGN Prospective cohort	502:532	STUDY DESIGN Prospective cohort study in a medical center.
30761540	9	58	theme	hyaluronate	1562:1572	arg1	injection					1574:1582	intracordal hyaluronate injection	1550:1582	intracordal hyaluronate injection	1550:1582	Seventy-one of all patients received early intervention with intracordal hyaluronate injection, showing similar therapeutic effects in those with and without CT involvement.
30761540	3	59	theme	STUDY	502:506	arg1	cohort					527:532	STUDY DESIGN Prospective cohort	502:532	STUDY DESIGN Prospective cohort	502:532	STUDY DESIGN Prospective cohort study in a medical center.
30761540	4	60	theme	UVFP	855:858	arg1	onset					860:864	UVFP onset	855:864	UVFP onset	855:864	METHODS Eighty-one patients with UVFP (37 males and 44 females) received an initial assessment of quantitative LEMG, stroboscope, acoustic voice analysis and 36-Item Short Form Survey quality-of-life questionnaire at 3 to 6 months after UVFP onset and a follow-up assessment at 12 months after UVFP onset.
30761540	10	61	theme	TA-LCA	1700:1705	arg1	complex					1714:1720	combined TA-LCA muscle complex and CT muscle involvement	1691:1746	complex	1714:1720	CONCLUSIONS Acute UVFP with combined TA-LCA muscle complex and CT muscle involvement has a poor prognosis, with poorer recovery of TA-LCA muscle complex recruitment.
30761540	10	62	theme	CT	1726:1727	arg1	involvement					1736:1746	combined TA-LCA muscle complex and CT muscle involvement	1691:1746	involvement	1736:1746	CONCLUSIONS Acute UVFP with combined TA-LCA muscle complex and CT muscle involvement has a poor prognosis, with poorer recovery of TA-LCA muscle complex recruitment.
30761540	9	63	theme	similar	1593:1599	arg1	effects					1613:1619	similar therapeutic effects	1593:1619	similar therapeutic effects	1593:1619	Seventy-one of all patients received early intervention with intracordal hyaluronate injection, showing similar therapeutic effects in those with and without CT involvement.
30761540	2	64	theme	present	264:270	arg1	study					272:276	The present study	260:276	The present study	260:276	The present study examined the impact of cricothyroid (CT) muscle involvement on recovery using quantitative laryngeal electromyography (LEMG) of the thyroarytenoid-lateral cricoarytenoid (TA-LCA) muscle complex at multiple times postinjury.
30761540	4	65	theme	initial	637:643	arg1	assessment					645:654	an initial assessment	634:654	an initial assessment of quantitative LEMG, stroboscope, acoustic voice analysis and 36-Item Short Form Survey quality-of-life questionnaire at 3 to 6 months after UVFP onset	634:807	METHODS Eighty-one patients with UVFP (37 males and 44 females) received an initial assessment of quantitative LEMG, stroboscope, acoustic voice analysis and 36-Item Short Form Survey quality-of-life questionnaire at 3 to 6 months after UVFP onset and a follow-up assessment at 12 months after UVFP onset.
30761540	2	66	theme	cricothyroid	301:312	arg1	involvement					326:336	cricothyroid (CT) muscle involvement	301:336	cricothyroid (CT) muscle involvement	301:336	The present study examined the impact of cricothyroid (CT) muscle involvement on recovery using quantitative laryngeal electromyography (LEMG) of the thyroarytenoid-lateral cricoarytenoid (TA-LCA) muscle complex at multiple times postinjury.
30761540	1	67	theme	vocal	195:199	arg1	UVFP					217:220	UVFP	217:220	UVFP	217:220	OBJECTIVES/HYPOTHESIS Wide variation in postinjury functional recovery is a hallmark of unilateral vocal fold paralysis (UVFP), ranging from zero to full recovery.
30761540	1	67	theme	vocal	195:199	arg1	paralysis					206:214	unilateral vocal fold paralysis	184:214	unilateral vocal fold paralysis (UVFP)	184:221	OBJECTIVES/HYPOTHESIS Wide variation in postinjury functional recovery is a hallmark of unilateral vocal fold paralysis (UVFP), ranging from zero to full recovery.
30761540	1	68	theme	paralysis	206:214	arg1	variation					123:131	OBJECTIVES/HYPOTHESIS Wide variation	96:131	OBJECTIVES/HYPOTHESIS Wide variation in postinjury functional recovery	96:165	OBJECTIVES/HYPOTHESIS Wide variation in postinjury functional recovery is a hallmark of unilateral vocal fold paralysis (UVFP), ranging from zero to full recovery.
30761540	1	68	theme	paralysis	206:214	arg1	hallmark					172:179	a hallmark	170:179	a hallmark	170:179	OBJECTIVES/HYPOTHESIS Wide variation in postinjury functional recovery is a hallmark of unilateral vocal fold paralysis (UVFP), ranging from zero to full recovery.
30761540	11	69	with	patients	1873:1880	arg1	UVFP					1887:1890	UVFP	1887:1890	UVFP	1887:1890	Early interventions should be considered in patients with UVFP with CT involvement.
30761540	11	69	with	patients	1873:1880	arg1	involvement					1900:1910	CT involvement	1897:1910	CT involvement	1897:1910	Early interventions should be considered in patients with UVFP with CT involvement.
30761540	11	70	theme	CT	1897:1898	arg1	involvement					1900:1910	CT involvement	1897:1910	CT involvement	1897:1910	Early interventions should be considered in patients with UVFP with CT involvement.
30761540	9	71	theme	CT	1647:1648	arg1	involvement					1650:1660	CT involvement	1647:1660	CT involvement	1647:1660	Seventy-one of all patients received early intervention with intracordal hyaluronate injection, showing similar therapeutic effects in those with and without CT involvement.
30761540	4	72	dep	patients	580:587	arg1	males					603:607	37 males	600:607	37 males	600:607	METHODS Eighty-one patients with UVFP (37 males and 44 females) received an initial assessment of quantitative LEMG, stroboscope, acoustic voice analysis and 36-Item Short Form Survey quality-of-life questionnaire at 3 to 6 months after UVFP onset and a follow-up assessment at 12 months after UVFP onset.
30761540	4	72	dep	patients	580:587	arg1	females					616:622	44 females	613:622	44 females	613:622	METHODS Eighty-one patients with UVFP (37 males and 44 females) received an initial assessment of quantitative LEMG, stroboscope, acoustic voice analysis and 36-Item Short Form Survey quality-of-life questionnaire at 3 to 6 months after UVFP onset and a follow-up assessment at 12 months after UVFP onset.
30761540	8	73	dep	511 ± 301 Hz	1384:1395	arg1	to					1381:1382	to	1381:1382	to	1381:1382	TA-LCA muscle complex turn frequency improved in patients without CT involvement (from 277 ± 198 to 511 ± 301 Hz; P < .001), but not in those with CT involvement (from 345 ± 211 to 386 ± 265 Hz; P = .46).
30761540	8	73	dep	511 ± 301 Hz	1384:1395	arg1	P					1398:1398	P < .001	1398:1405	277 ± 198 to 511 ± 301 Hz; P < .001	1371:1405	TA-LCA muscle complex turn frequency improved in patients without CT involvement (from 277 ± 198 to 511 ± 301 Hz; P < .001), but not in those with CT involvement (from 345 ± 211 to 386 ± 265 Hz; P = .46).
30761540	4	74	theme	acoustic	691:698	arg1	analysis					706:713	acoustic voice analysis	691:713	acoustic voice analysis	691:713	METHODS Eighty-one patients with UVFP (37 males and 44 females) received an initial assessment of quantitative LEMG, stroboscope, acoustic voice analysis and 36-Item Short Form Survey quality-of-life questionnaire at 3 to 6 months after UVFP onset and a follow-up assessment at 12 months after UVFP onset.
30761540	1	75	theme	postinjury	136:145	arg1	recovery					158:165	postinjury functional recovery	136:165	postinjury functional recovery	136:165	OBJECTIVES/HYPOTHESIS Wide variation in postinjury functional recovery is a hallmark of unilateral vocal fold paralysis (UVFP), ranging from zero to full recovery.
30761540	6	76	theme	TA-LCA	1026:1031	arg1	complex					1040:1046	the TA-LCA muscle complex	1022:1046	the TA-LCA muscle complex	1022:1046	The peak turn frequency of the TA-LCA muscle complex on the lesion side was improved at the follow-up (470 ± 294 Hz) compared with the initial assessment (300 ± 204 Hz) (P < .001).
30761540	8	77	theme	muscle	1291:1296	arg1	frequency					1311:1319	TA-LCA muscle complex turn frequency	1284:1319	TA-LCA muscle complex turn frequency	1284:1319	TA-LCA muscle complex turn frequency improved in patients without CT involvement (from 277 ± 198 to 511 ± 301 Hz; P < .001), but not in those with CT involvement (from 345 ± 211 to 386 ± 265 Hz; P = .46).
30761540	4	78	theme	analysis	706:713	arg1	assessment					645:654	an initial assessment	634:654	an initial assessment of quantitative LEMG, stroboscope, acoustic voice analysis and 36-Item Short Form Survey quality-of-life questionnaire at 3 to 6 months after UVFP onset	634:807	METHODS Eighty-one patients with UVFP (37 males and 44 females) received an initial assessment of quantitative LEMG, stroboscope, acoustic voice analysis and 36-Item Short Form Survey quality-of-life questionnaire at 3 to 6 months after UVFP onset and a follow-up assessment at 12 months after UVFP onset.
30761540	4	78	theme	analysis	706:713	arg1	assessment					825:834	a follow-up assessment	813:834	a follow-up assessment at 12 months after UVFP onset	813:864	METHODS Eighty-one patients with UVFP (37 males and 44 females) received an initial assessment of quantitative LEMG, stroboscope, acoustic voice analysis and 36-Item Short Form Survey quality-of-life questionnaire at 3 to 6 months after UVFP onset and a follow-up assessment at 12 months after UVFP onset.
30761540	0	79	theme	unilateral	63:72	arg1	paralysis					85:93	unilateral vocal fold paralysis	63:93	unilateral vocal fold paralysis	63:93	The impact of cricothyroid involvement on adductor recovery in unilateral vocal fold paralysis.
30761540	8	80	dep	386 ± 265 Hz	1465:1476	arg1	P					1479:1479	P = .46	1479:1485	345 ± 211 to 386 ± 265 Hz; P = .46	1452:1485	TA-LCA muscle complex turn frequency improved in patients without CT involvement (from 277 ± 198 to 511 ± 301 Hz; P < .001), but not in those with CT involvement (from 345 ± 211 to 386 ± 265 Hz; P = .46).
30761540	8	80	dep	386 ± 265 Hz	1465:1476	arg1	to					1462:1463	to	1462:1463	to	1462:1463	TA-LCA muscle complex turn frequency improved in patients without CT involvement (from 277 ± 198 to 511 ± 301 Hz; P < .001), but not in those with CT involvement (from 345 ± 211 to 386 ± 265 Hz; P = .46).
30761540	2	81	theme	postinjury	490:499	arg1	times					484:488	multiple times	475:488	multiple times postinjury	475:499	The present study examined the impact of cricothyroid (CT) muscle involvement on recovery using quantitative laryngeal electromyography (LEMG) of the thyroarytenoid-lateral cricoarytenoid (TA-LCA) muscle complex at multiple times postinjury.
30761540	4	82	theme	LEMG	672:675	arg1	assessment					645:654	an initial assessment	634:654	an initial assessment of quantitative LEMG, stroboscope, acoustic voice analysis and 36-Item Short Form Survey quality-of-life questionnaire at 3 to 6 months after UVFP onset	634:807	METHODS Eighty-one patients with UVFP (37 males and 44 females) received an initial assessment of quantitative LEMG, stroboscope, acoustic voice analysis and 36-Item Short Form Survey quality-of-life questionnaire at 3 to 6 months after UVFP onset and a follow-up assessment at 12 months after UVFP onset.
30761540	4	82	theme	LEMG	672:675	arg1	assessment					825:834	a follow-up assessment	813:834	a follow-up assessment at 12 months after UVFP onset	813:864	METHODS Eighty-one patients with UVFP (37 males and 44 females) received an initial assessment of quantitative LEMG, stroboscope, acoustic voice analysis and 36-Item Short Form Survey quality-of-life questionnaire at 3 to 6 months after UVFP onset and a follow-up assessment at 12 months after UVFP onset.
30761540	0	83	theme	fold	80:83	arg1	paralysis					85:93	unilateral vocal fold paralysis	63:93	unilateral vocal fold paralysis	63:93	The impact of cricothyroid involvement on adductor recovery in unilateral vocal fold paralysis.
30761540	6	84	theme	turn	1004:1007	arg1	frequency					1009:1017	The peak turn frequency	995:1017	The peak turn frequency of the TA-LCA muscle complex on the lesion side	995:1065	The peak turn frequency of the TA-LCA muscle complex on the lesion side was improved at the follow-up (470 ± 294 Hz) compared with the initial assessment (300 ± 204 Hz) (P < .001).
30761540	2	85	theme	multiple	475:482	arg1	times					484:488	multiple times	475:488	multiple times postinjury	475:499	The present study examined the impact of cricothyroid (CT) muscle involvement on recovery using quantitative laryngeal electromyography (LEMG) of the thyroarytenoid-lateral cricoarytenoid (TA-LCA) muscle complex at multiple times postinjury.
30761540	0	86	from	impact	4:9	arg1	recovery					51:58	adductor recovery	42:58	adductor recovery in unilateral vocal fold paralysis	42:93	The impact of cricothyroid involvement on adductor recovery in unilateral vocal fold paralysis.
30761540	6	87	from	frequency	1009:1017	arg1	side					1062:1065	the lesion side	1051:1065	the lesion side	1051:1065	The peak turn frequency of the TA-LCA muscle complex on the lesion side was improved at the follow-up (470 ± 294 Hz) compared with the initial assessment (300 ± 204 Hz) (P < .001).
30761540	4	88	theme	stroboscope	678:688	arg1	assessment					645:654	an initial assessment	634:654	an initial assessment of quantitative LEMG, stroboscope, acoustic voice analysis and 36-Item Short Form Survey quality-of-life questionnaire at 3 to 6 months after UVFP onset	634:807	METHODS Eighty-one patients with UVFP (37 males and 44 females) received an initial assessment of quantitative LEMG, stroboscope, acoustic voice analysis and 36-Item Short Form Survey quality-of-life questionnaire at 3 to 6 months after UVFP onset and a follow-up assessment at 12 months after UVFP onset.
30761540	4	88	theme	stroboscope	678:688	arg1	assessment					825:834	a follow-up assessment	813:834	a follow-up assessment at 12 months after UVFP onset	813:864	METHODS Eighty-one patients with UVFP (37 males and 44 females) received an initial assessment of quantitative LEMG, stroboscope, acoustic voice analysis and 36-Item Short Form Survey quality-of-life questionnaire at 3 to 6 months after UVFP onset and a follow-up assessment at 12 months after UVFP onset.
30761540	5	89	theme	UVFP	969:972	arg1	onset					974:978	UVFP onset	969:978	UVFP onset	969:978	RESULTS The initial and follow-up assessments were performed at 4.3 ± 1.9 and 12.5 ± 1.3 months after UVFP onset, respectively.
30761540	10	90	theme	muscle	1707:1712	arg1	complex					1714:1720	combined TA-LCA muscle complex and CT muscle involvement	1691:1746	complex	1714:1720	CONCLUSIONS Acute UVFP with combined TA-LCA muscle complex and CT muscle involvement has a poor prognosis, with poorer recovery of TA-LCA muscle complex recruitment.
30761540	0	91	from	recovery	51:58	arg1	paralysis					85:93	unilateral vocal fold paralysis	63:93	unilateral vocal fold paralysis	63:93	The impact of cricothyroid involvement on adductor recovery in unilateral vocal fold paralysis.
30761540	8	92	theme	TA-LCA	1284:1289	arg1	frequency					1311:1319	TA-LCA muscle complex turn frequency	1284:1319	TA-LCA muscle complex turn frequency	1284:1319	TA-LCA muscle complex turn frequency improved in patients without CT involvement (from 277 ± 198 to 511 ± 301 Hz; P < .001), but not in those with CT involvement (from 345 ± 211 to 386 ± 265 Hz; P = .46).
30761540	4	93	theme	quality-of-life	745:759	arg1	questionnaire					761:773	36-Item Short Form Survey quality-of-life questionnaire	719:773	36-Item Short Form Survey quality-of-life questionnaire at 3 to 6 months after UVFP onset	719:807	METHODS Eighty-one patients with UVFP (37 males and 44 females) received an initial assessment of quantitative LEMG, stroboscope, acoustic voice analysis and 36-Item Short Form Survey quality-of-life questionnaire at 3 to 6 months after UVFP onset and a follow-up assessment at 12 months after UVFP onset.
30761540	4	94	from	12 months	839:847	arg1	assessment					645:654	an initial assessment	634:654	an initial assessment of quantitative LEMG, stroboscope, acoustic voice analysis and 36-Item Short Form Survey quality-of-life questionnaire at 3 to 6 months after UVFP onset	634:807	METHODS Eighty-one patients with UVFP (37 males and 44 females) received an initial assessment of quantitative LEMG, stroboscope, acoustic voice analysis and 36-Item Short Form Survey quality-of-life questionnaire at 3 to 6 months after UVFP onset and a follow-up assessment at 12 months after UVFP onset.
30761540	4	94	from	12 months	839:847	arg1	assessment					825:834	a follow-up assessment	813:834	a follow-up assessment at 12 months after UVFP onset	813:864	METHODS Eighty-one patients with UVFP (37 males and 44 females) received an initial assessment of quantitative LEMG, stroboscope, acoustic voice analysis and 36-Item Short Form Survey quality-of-life questionnaire at 3 to 6 months after UVFP onset and a follow-up assessment at 12 months after UVFP onset.
30761540	8	95	theme	complex	1298:1304	arg1	frequency					1311:1319	TA-LCA muscle complex turn frequency	1284:1319	TA-LCA muscle complex turn frequency	1284:1319	TA-LCA muscle complex turn frequency improved in patients without CT involvement (from 277 ± 198 to 511 ± 301 Hz; P < .001), but not in those with CT involvement (from 345 ± 211 to 386 ± 265 Hz; P = .46).
30761540	4	96	from	assessment	825:834	arg1	3					778:778	3	778:778	3	778:778	METHODS Eighty-one patients with UVFP (37 males and 44 females) received an initial assessment of quantitative LEMG, stroboscope, acoustic voice analysis and 36-Item Short Form Survey quality-of-life questionnaire at 3 to 6 months after UVFP onset and a follow-up assessment at 12 months after UVFP onset.
30761540	4	97	theme	36-Item	719:725	arg1	questionnaire					761:773	36-Item Short Form Survey quality-of-life questionnaire	719:773	36-Item Short Form Survey quality-of-life questionnaire at 3 to 6 months after UVFP onset	719:807	METHODS Eighty-one patients with UVFP (37 males and 44 females) received an initial assessment of quantitative LEMG, stroboscope, acoustic voice analysis and 36-Item Short Form Survey quality-of-life questionnaire at 3 to 6 months after UVFP onset and a follow-up assessment at 12 months after UVFP onset.
30761540	2	98	theme	quantitative	356:367	arg1	electromyography					379:394	quantitative laryngeal electromyography	356:394	quantitative laryngeal electromyography (LEMG) of the thyroarytenoid-lateral cricoarytenoid (TA-LCA) muscle complex	356:470	The present study examined the impact of cricothyroid (CT) muscle involvement on recovery using quantitative laryngeal electromyography (LEMG) of the thyroarytenoid-lateral cricoarytenoid (TA-LCA) muscle complex at multiple times postinjury.
30761540	2	98	theme	quantitative	356:367	arg1	LEMG					397:400	LEMG	397:400	LEMG	397:400	The present study examined the impact of cricothyroid (CT) muscle involvement on recovery using quantitative laryngeal electromyography (LEMG) of the thyroarytenoid-lateral cricoarytenoid (TA-LCA) muscle complex at multiple times postinjury.
30761540	0	99	theme	cricothyroid	14:25	arg1	involvement					27:37	cricothyroid involvement	14:37	cricothyroid involvement	14:37	The impact of cricothyroid involvement on adductor recovery in unilateral vocal fold paralysis.
30761540	1	100	theme	OBJECTIVES/HYPOTHESIS	96:116	arg1	variation					123:131	OBJECTIVES/HYPOTHESIS Wide variation	96:131	OBJECTIVES/HYPOTHESIS Wide variation in postinjury functional recovery	96:165	OBJECTIVES/HYPOTHESIS Wide variation in postinjury functional recovery is a hallmark of unilateral vocal fold paralysis (UVFP), ranging from zero to full recovery.
30761540	1	100	theme	OBJECTIVES/HYPOTHESIS	96:116	arg1	hallmark					172:179	a hallmark	170:179	a hallmark	170:179	OBJECTIVES/HYPOTHESIS Wide variation in postinjury functional recovery is a hallmark of unilateral vocal fold paralysis (UVFP), ranging from zero to full recovery.
30761540	2	101	theme	thyroarytenoid-lateral	410:431	arg1	complex					464:470	the thyroarytenoid-lateral cricoarytenoid (TA-LCA) muscle complex	406:470	the thyroarytenoid-lateral cricoarytenoid (TA-LCA) muscle complex	406:470	The present study examined the impact of cricothyroid (CT) muscle involvement on recovery using quantitative laryngeal electromyography (LEMG) of the thyroarytenoid-lateral cricoarytenoid (TA-LCA) muscle complex at multiple times postinjury.
30761540	4	102	theme	Eighty-one	569:578	arg1	patients					580:587	METHODS Eighty-one patients	561:587	METHODS Eighty-one patients with UVFP (37 males and 44 females)	561:623	METHODS Eighty-one patients with UVFP (37 males and 44 females) received an initial assessment of quantitative LEMG, stroboscope, acoustic voice analysis and 36-Item Short Form Survey quality-of-life questionnaire at 3 to 6 months after UVFP onset and a follow-up assessment at 12 months after UVFP onset.
30761540	10	103	theme	TA-LCA	1794:1799	arg1	recruitment					1816:1826	TA-LCA muscle complex recruitment	1794:1826	TA-LCA muscle complex recruitment	1794:1826	CONCLUSIONS Acute UVFP with combined TA-LCA muscle complex and CT muscle involvement has a poor prognosis, with poorer recovery of TA-LCA muscle complex recruitment.
30761540	4	104	theme	Form	733:736	arg1	questionnaire					761:773	36-Item Short Form Survey quality-of-life questionnaire	719:773	36-Item Short Form Survey quality-of-life questionnaire at 3 to 6 months after UVFP onset	719:807	METHODS Eighty-one patients with UVFP (37 males and 44 females) received an initial assessment of quantitative LEMG, stroboscope, acoustic voice analysis and 36-Item Short Form Survey quality-of-life questionnaire at 3 to 6 months after UVFP onset and a follow-up assessment at 12 months after UVFP onset.
30761540	10	105	theme	Acute	1675:1679	arg1	UVFP					1681:1684	CONCLUSIONS Acute UVFP	1663:1684	CONCLUSIONS Acute UVFP with combined TA-LCA muscle complex and CT muscle involvement	1663:1746	CONCLUSIONS Acute UVFP with combined TA-LCA muscle complex and CT muscle involvement has a poor prognosis, with poorer recovery of TA-LCA muscle complex recruitment.
30761540	6	106	theme	complex	1040:1046	arg1	frequency					1009:1017	The peak turn frequency	995:1017	The peak turn frequency of the TA-LCA muscle complex on the lesion side	995:1065	The peak turn frequency of the TA-LCA muscle complex on the lesion side was improved at the follow-up (470 ± 294 Hz) compared with the initial assessment (300 ± 204 Hz) (P < .001).
30761540	2	107	theme	complex	464:470	arg1	electromyography					379:394	quantitative laryngeal electromyography	356:394	quantitative laryngeal electromyography (LEMG) of the thyroarytenoid-lateral cricoarytenoid (TA-LCA) muscle complex	356:470	The present study examined the impact of cricothyroid (CT) muscle involvement on recovery using quantitative laryngeal electromyography (LEMG) of the thyroarytenoid-lateral cricoarytenoid (TA-LCA) muscle complex at multiple times postinjury.
30761540	2	107	theme	complex	464:470	arg1	LEMG					397:400	LEMG	397:400	LEMG	397:400	The present study examined the impact of cricothyroid (CT) muscle involvement on recovery using quantitative laryngeal electromyography (LEMG) of the thyroarytenoid-lateral cricoarytenoid (TA-LCA) muscle complex at multiple times postinjury.
30761540	5	108	dep	RESULTS	867:873	arg1	performed					918:926	performed	918:926	performed	918:926	RESULTS The initial and follow-up assessments were performed at 4.3 ± 1.9 and 12.5 ± 1.3 months after UVFP onset, respectively.
30761540	10	109	theme	complex	1808:1814	arg1	recruitment					1816:1826	TA-LCA muscle complex recruitment	1794:1826	TA-LCA muscle complex recruitment	1794:1826	CONCLUSIONS Acute UVFP with combined TA-LCA muscle complex and CT muscle involvement has a poor prognosis, with poorer recovery of TA-LCA muscle complex recruitment.
30761540	9	110	theme	intracordal	1550:1560	arg1	injection					1574:1582	intracordal hyaluronate injection	1550:1582	intracordal hyaluronate injection	1550:1582	Seventy-one of all patients received early intervention with intracordal hyaluronate injection, showing similar therapeutic effects in those with and without CT involvement.
30761540	10	111	theme	CONCLUSIONS	1663:1673	arg1	UVFP					1681:1684	CONCLUSIONS Acute UVFP	1663:1684	CONCLUSIONS Acute UVFP with combined TA-LCA muscle complex and CT muscle involvement	1663:1746	CONCLUSIONS Acute UVFP with combined TA-LCA muscle complex and CT muscle involvement has a poor prognosis, with poorer recovery of TA-LCA muscle complex recruitment.
29314593	6	0	theme	freeze-dried	1188:1199	arg1	ones					1201:1204	the freeze-dried ones	1184:1204	the freeze-dried ones	1184:1204	Naturally dried CS/SiO2 hybrid provoked a more severe inflammation than the freeze-dried ones.
29314593	4	1	theme	electron	824:831	arg1	microscopy					833:842	transmission electron microscopy	811:842	transmission electron microscopy	811:842	Scanning electron microscopy demonstrated the hybrids possessed the uniform geometric structure, while, transmission electron microscopy displayed nanoscale silica, or HA nanoparticles dispersed homogeneously in the CS matrix, or CS/silica hybrids.
29314593	2	2	theme	hybrids	383:389	arg1	immuno-reactivity					352:368	the precise in vivo immuno-reactivity	332:368	the precise in vivo immuno-reactivity of silica-CS hybrids with various compositions	332:415	However, the precise in vivo immuno-reactivity of silica-CS hybrids with various compositions is still poorly defined.
29314593	4	3	theme	CS/silica	937:945	arg1	hybrids					947:953	CS/silica hybrids	937:953	CS/silica hybrids	937:953	Scanning electron microscopy demonstrated the hybrids possessed the uniform geometric structure, while, transmission electron microscopy displayed nanoscale silica, or HA nanoparticles dispersed homogeneously in the CS matrix, or CS/silica hybrids.
29314593	1	4	theme	silica-chitosan	149:163	arg1	scaffolds					170:178	Inorganic/organic hybrid silica-chitosan (CS) scaffolds	124:178	Inorganic/organic hybrid silica-chitosan (CS) scaffolds	124:178	Inorganic/organic hybrid silica-chitosan (CS) scaffolds have promising potential for bone defect repair, due to the controllable mechanical properties, degradation behavior, and scaffold morphology.
29314593	7	5	from	absence	1348:1354	arg1	muscle					1392:1397	muscle	1392:1397	muscle	1392:1397	Dendritic cells were attracted to invade into the implants embedded-muscle, but not be activated to prime the adaptive immunity, because the absence of cytotoxic T cells and B cells in muscle received the implants.
29314593	1	6	contain	have	180:183	arg2	potential					195:203	promising potential	185:203	promising potential for bone defect repair, due to the controllable mechanical properties, degradation behavior, and scaffold morphology	185:320	Inorganic/organic hybrid silica-chitosan (CS) scaffolds have promising potential for bone defect repair, due to the controllable mechanical properties, degradation behavior, and scaffold morphology.
29314593	1	6	contain	have	180:183	arg1	scaffolds					170:178	Inorganic/organic hybrid silica-chitosan (CS) scaffolds	124:178	Inorganic/organic hybrid silica-chitosan (CS) scaffolds	124:178	Inorganic/organic hybrid silica-chitosan (CS) scaffolds have promising potential for bone defect repair, due to the controllable mechanical properties, degradation behavior, and scaffold morphology.
29314593	3	7	dep	three-dimensional	475:491	arg1	3D					494:495	3D	494:495	3D	494:495	In this study, we fabricated the three-dimensional (3D) interconnected porous chitosan-silica (CS/SiO2 ) and chitosan-silica-hydroxyapatite (CS/SiO2 /HA) hybrids, through sol-gel process and 3D plotting skill, followed by the naturally or freeze drying separately.
29314593	3	8	theme	CS/SiO2	583:589	arg1	hybrids					596:602	interconnected porous chitosan-silica (CS/SiO2 ) and chitosan-silica-hydroxyapatite (CS/SiO2 /HA) hybrids	498:602	hybrids	596:602	In this study, we fabricated the three-dimensional (3D) interconnected porous chitosan-silica (CS/SiO2 ) and chitosan-silica-hydroxyapatite (CS/SiO2 /HA) hybrids, through sol-gel process and 3D plotting skill, followed by the naturally or freeze drying separately.
29314593	1	9	theme	bone	209:212	arg1	repair					221:226	bone defect repair	209:226	bone defect repair	209:226	Inorganic/organic hybrid silica-chitosan (CS) scaffolds have promising potential for bone defect repair, due to the controllable mechanical properties, degradation behavior, and scaffold morphology.
29314593	4	10	theme	CS	923:924	arg1	matrix					926:931	the CS matrix	919:931	the CS matrix	919:931	Scanning electron microscopy demonstrated the hybrids possessed the uniform geometric structure, while, transmission electron microscopy displayed nanoscale silica, or HA nanoparticles dispersed homogeneously in the CS matrix, or CS/silica hybrids.
29314593	4	11	theme	Scanning	707:714	arg1	microscopy					725:734	Scanning electron microscopy	707:734	Scanning electron microscopy	707:734	Scanning electron microscopy demonstrated the hybrids possessed the uniform geometric structure, while, transmission electron microscopy displayed nanoscale silica, or HA nanoparticles dispersed homogeneously in the CS matrix, or CS/silica hybrids.
29314593	12	12	dep	106A	2024:2027	arg1	1223-1235					2030:2038	1223-1235	2030:2038	1223-1235	2030:2038	J Biomed Mater Res Part A: 106A: 1223-1235, 2018.
29314593	2	13	theme	precise	336:342	arg1	immuno-reactivity					352:368	the precise in vivo immuno-reactivity	332:368	the precise in vivo immuno-reactivity of silica-CS hybrids with various compositions	332:415	However, the precise in vivo immuno-reactivity of silica-CS hybrids with various compositions is still poorly defined.
29314593	9	14	theme	Plasma	1557:1562	arg1	assay					1620:1624	Plasma complement C3 enzyme linked immunosorbent assay (ELISA) assay	1557:1624	Plasma complement C3 enzyme linked immunosorbent assay (ELISA) assay	1557:1624	Plasma complement C3 enzyme linked immunosorbent assay (ELISA) assay showed the hybrids induced C3 levels increase in early implanting phase, and the subsequent striking decrease.
29314593	10	15	theme	plotted	1789:1795	arg1	CS/SiO2					1804:1810	3D plotted porous CS/SiO2 and CS/SiO2 /HA hybrids	1786:1834	CS/SiO2	1804:1810	Thus, the present results suggest that, in vivo, 3D plotted porous CS/SiO2 and CS/SiO2 /HA hybrids are relatively biocompatible in vivo, which initiate a localized inflammatory procedure, instead of a systematic immune response.
29314593	1	16	theme	due	229:231	arg1	repair					221:226	bone defect repair	209:226	bone defect repair	209:226	Inorganic/organic hybrid silica-chitosan (CS) scaffolds have promising potential for bone defect repair, due to the controllable mechanical properties, degradation behavior, and scaffold morphology.
29314593	12	17	theme	Part	2016:2019	arg1	A					2021:2021	Part A	2016:2021	J Biomed Mater Res Part A: 106A: 1223-1235, 2018.	1997:2045	J Biomed Mater Res Part A: 106A: 1223-1235, 2018.
29314593	8	18	theme	splenocytes	1533:1543	arg1	activation					1545:1554	splenocytes activation	1533:1554	splenocytes activation	1533:1554	Fluorescence-activated cell sorting (FACS) analysis indicated the implanted hybrids were incapable to initiate splenocytes activation.
29314593	3	19	theme	plotting	636:643	arg1	skill					645:649	3D plotting skill	633:649	3D plotting skill	633:649	In this study, we fabricated the three-dimensional (3D) interconnected porous chitosan-silica (CS/SiO2 ) and chitosan-silica-hydroxyapatite (CS/SiO2 /HA) hybrids, through sol-gel process and 3D plotting skill, followed by the naturally or freeze drying separately.
29314593	9	20	theme	linked	1585:1590	arg1	assay					1620:1624	Plasma complement C3 enzyme linked immunosorbent assay (ELISA) assay	1557:1624	Plasma complement C3 enzyme linked immunosorbent assay (ELISA) assay	1557:1624	Plasma complement C3 enzyme linked immunosorbent assay (ELISA) assay showed the hybrids induced C3 levels increase in early implanting phase, and the subsequent striking decrease.
29314593	9	21	theme	assay	1606:1610	arg1	assay					1620:1624	Plasma complement C3 enzyme linked immunosorbent assay (ELISA) assay	1557:1624	Plasma complement C3 enzyme linked immunosorbent assay (ELISA) assay	1557:1624	Plasma complement C3 enzyme linked immunosorbent assay (ELISA) assay showed the hybrids induced C3 levels increase in early implanting phase, and the subsequent striking decrease.
29314593	4	22	theme	uniform	775:781	arg1	structure					793:801	the uniform geometric structure	771:801	the uniform geometric structure	771:801	Scanning electron microscopy demonstrated the hybrids possessed the uniform geometric structure, while, transmission electron microscopy displayed nanoscale silica, or HA nanoparticles dispersed homogeneously in the CS matrix, or CS/silica hybrids.
29314593	9	23	theme	early	1675:1679	arg1	phase					1692:1696	early implanting phase	1675:1696	early implanting phase	1675:1696	Plasma complement C3 enzyme linked immunosorbent assay (ELISA) assay showed the hybrids induced C3 levels increase in early implanting phase, and the subsequent striking decrease.
29314593	9	24	theme	ELISA	1613:1617	arg1	assay					1620:1624	Plasma complement C3 enzyme linked immunosorbent assay (ELISA) assay	1557:1624	Plasma complement C3 enzyme linked immunosorbent assay (ELISA) assay	1557:1624	Plasma complement C3 enzyme linked immunosorbent assay (ELISA) assay showed the hybrids induced C3 levels increase in early implanting phase, and the subsequent striking decrease.
29314593	9	25	from	increase	1663:1670	arg1	phase					1692:1696	early implanting phase	1675:1696	early implanting phase	1675:1696	Plasma complement C3 enzyme linked immunosorbent assay (ELISA) assay showed the hybrids induced C3 levels increase in early implanting phase, and the subsequent striking decrease.
29314593	2	26	dep	in	344:345	arg1	vivo					347:350	vivo	347:350	vivo	347:350	However, the precise in vivo immuno-reactivity of silica-CS hybrids with various compositions is still poorly defined.
29314593	0	27	theme	chitosan/SiO2	67:79	arg1	analysis					26:33	In vivo immuno-reactivity analysis	0:33	In vivo immuno-reactivity analysis of the porous three-dimensional chitosan/SiO2 and chitosan/SiO2	0:97	In vivo immuno-reactivity analysis of the porous three-dimensional chitosan/SiO2 and chitosan/SiO2 /hydroxyapatite hybrids.
29314593	10	28	theme	localized	1891:1899	arg1	procedure					1914:1922	a localized inflammatory procedure	1889:1922	a localized inflammatory procedure	1889:1922	Thus, the present results suggest that, in vivo, 3D plotted porous CS/SiO2 and CS/SiO2 /HA hybrids are relatively biocompatible in vivo, which initiate a localized inflammatory procedure, instead of a systematic immune response.
29314593	10	29	from	biocompatible	1851:1863	arg1	vivo					1868:1871	vivo	1868:1871	vivo	1868:1871	Thus, the present results suggest that, in vivo, 3D plotted porous CS/SiO2 and CS/SiO2 /HA hybrids are relatively biocompatible in vivo, which initiate a localized inflammatory procedure, instead of a systematic immune response.
29314593	0	30	theme	chitosan/SiO2	85:97	arg1	analysis					26:33	In vivo immuno-reactivity analysis	0:33	In vivo immuno-reactivity analysis of the porous three-dimensional chitosan/SiO2 and chitosan/SiO2	0:97	In vivo immuno-reactivity analysis of the porous three-dimensional chitosan/SiO2 and chitosan/SiO2 /hydroxyapatite hybrids.
29314593	9	31	link	linked	1585:1590	arg1	assay					1620:1624	Plasma complement C3 enzyme linked immunosorbent assay (ELISA) assay	1557:1624	Plasma complement C3 enzyme linked immunosorbent assay (ELISA) assay	1557:1624	Plasma complement C3 enzyme linked immunosorbent assay (ELISA) assay showed the hybrids induced C3 levels increase in early implanting phase, and the subsequent striking decrease.
29314593	5	32	theme	intramuscular	962:974	arg1	implantation					976:987	intramuscular implantation	962:987	intramuscular implantation	962:987	After intramuscular implantation, CS/SiO2 and CS/SiO2 /HA hybrids triggered a local and limited monocyte/macrophage infiltration and myofiber degeneration.
29314593	10	33	from	vivo	1868:1871	arg1	biocompatible					1851:1863	biocompatible	1851:1863	biocompatible	1851:1863	Thus, the present results suggest that, in vivo, 3D plotted porous CS/SiO2 and CS/SiO2 /HA hybrids are relatively biocompatible in vivo, which initiate a localized inflammatory procedure, instead of a systematic immune response.
29314593	8	34	theme	Fluorescence-activated	1422:1443	arg1	FACS					1459:1462	FACS	1459:1462	FACS	1459:1462	Fluorescence-activated cell sorting (FACS) analysis indicated the implanted hybrids were incapable to initiate splenocytes activation.
29314593	8	34	theme	Fluorescence-activated	1422:1443	arg1	sorting					1450:1456	Fluorescence-activated cell sorting	1422:1456	Fluorescence-activated cell sorting (FACS) analysis	1422:1472	Fluorescence-activated cell sorting (FACS) analysis indicated the implanted hybrids were incapable to initiate splenocytes activation.
29314593	9	35	theme	implanting	1681:1690	arg1	phase					1692:1696	early implanting phase	1675:1696	early implanting phase	1675:1696	Plasma complement C3 enzyme linked immunosorbent assay (ELISA) assay showed the hybrids induced C3 levels increase in early implanting phase, and the subsequent striking decrease.
29314593	11	36	dep	©	1966:1966	arg1	Inc.					1992:1995	Inc.	1992:1995	Inc.	1992:1995	© 2018 Wiley Periodicals, Inc.
29314593	5	37	theme	local	1034:1038	arg1	infiltration					1072:1083	a local and limited monocyte/macrophage infiltration	1032:1083	a local and limited monocyte/macrophage infiltration	1032:1083	After intramuscular implantation, CS/SiO2 and CS/SiO2 /HA hybrids triggered a local and limited monocyte/macrophage infiltration and myofiber degeneration.
29314593	9	38	from	decrease	1727:1734	arg1	phase					1692:1696	early implanting phase	1675:1696	early implanting phase	1675:1696	Plasma complement C3 enzyme linked immunosorbent assay (ELISA) assay showed the hybrids induced C3 levels increase in early implanting phase, and the subsequent striking decrease.
29314593	8	39	theme	sorting	1450:1456	arg1	analysis					1465:1472	Fluorescence-activated cell sorting (FACS) analysis	1422:1472	Fluorescence-activated cell sorting (FACS) analysis	1422:1472	Fluorescence-activated cell sorting (FACS) analysis indicated the implanted hybrids were incapable to initiate splenocytes activation.
29314593	10	40	theme	present	1747:1753	arg1	results					1755:1761	the present results	1743:1761	the present results	1743:1761	Thus, the present results suggest that, in vivo, 3D plotted porous CS/SiO2 and CS/SiO2 /HA hybrids are relatively biocompatible in vivo, which initiate a localized inflammatory procedure, instead of a systematic immune response.
29314593	5	41	theme	limited	1044:1050	arg1	infiltration					1072:1083	a local and limited monocyte/macrophage infiltration	1032:1083	a local and limited monocyte/macrophage infiltration	1032:1083	After intramuscular implantation, CS/SiO2 and CS/SiO2 /HA hybrids triggered a local and limited monocyte/macrophage infiltration and myofiber degeneration.
29314593	9	42	dep	showed	1626:1631	arg1	induced					1645:1651	induced	1645:1651	showed the hybrids induced C3 levels increase in early implanting phase, and the subsequent striking decrease	1626:1734	Plasma complement C3 enzyme linked immunosorbent assay (ELISA) assay showed the hybrids induced C3 levels increase in early implanting phase, and the subsequent striking decrease.
29314593	0	43	theme	In	0:1	arg1	analysis					26:33	In vivo immuno-reactivity analysis	0:33	In vivo immuno-reactivity analysis of the porous three-dimensional chitosan/SiO2 and chitosan/SiO2	0:97	In vivo immuno-reactivity analysis of the porous three-dimensional chitosan/SiO2 and chitosan/SiO2 /hydroxyapatite hybrids.
29314593	1	44	theme	scaffold	302:309	arg1	morphology					311:320	scaffold morphology	302:320	scaffold morphology	302:320	Inorganic/organic hybrid silica-chitosan (CS) scaffolds have promising potential for bone defect repair, due to the controllable mechanical properties, degradation behavior, and scaffold morphology.
29314593	5	45	theme	/HA	1010:1012	arg1	hybrids					1014:1020	CS/SiO2 /HA hybrids	1002:1020	CS/SiO2 /HA hybrids	1002:1020	After intramuscular implantation, CS/SiO2 and CS/SiO2 /HA hybrids triggered a local and limited monocyte/macrophage infiltration and myofiber degeneration.
29314593	0	46	theme	immuno-reactivity	8:24	arg1	analysis					26:33	In vivo immuno-reactivity analysis	0:33	In vivo immuno-reactivity analysis of the porous three-dimensional chitosan/SiO2 and chitosan/SiO2	0:97	In vivo immuno-reactivity analysis of the porous three-dimensional chitosan/SiO2 and chitosan/SiO2 /hydroxyapatite hybrids.
29314593	2	47	with	immuno-reactivity	352:368	arg1	compositions					404:415	various compositions	396:415	various compositions	396:415	However, the precise in vivo immuno-reactivity of silica-CS hybrids with various compositions is still poorly defined.
29314593	1	48	theme	hybrid	142:147	arg1	CS					166:167	CS	166:167	CS	166:167	Inorganic/organic hybrid silica-chitosan (CS) scaffolds have promising potential for bone defect repair, due to the controllable mechanical properties, degradation behavior, and scaffold morphology.
29314593	1	48	theme	hybrid	142:147	arg1	silica-chitosan					149:163	Inorganic/organic hybrid silica-chitosan	124:163	Inorganic/organic hybrid silica-chitosan (CS) scaffolds	124:178	Inorganic/organic hybrid silica-chitosan (CS) scaffolds have promising potential for bone defect repair, due to the controllable mechanical properties, degradation behavior, and scaffold morphology.
29314593	3	49	theme	freeze	681:686	arg1	drying					688:693	freeze drying	681:693	freeze drying	681:693	In this study, we fabricated the three-dimensional (3D) interconnected porous chitosan-silica (CS/SiO2 ) and chitosan-silica-hydroxyapatite (CS/SiO2 /HA) hybrids, through sol-gel process and 3D plotting skill, followed by the naturally or freeze drying separately.
29314593	4	50	theme	HA	875:876	arg1	nanoparticles					878:890	HA nanoparticles	875:890	HA nanoparticles	875:890	Scanning electron microscopy demonstrated the hybrids possessed the uniform geometric structure, while, transmission electron microscopy displayed nanoscale silica, or HA nanoparticles dispersed homogeneously in the CS matrix, or CS/silica hybrids.
29314593	3	51	theme	three-dimensional	475:491	arg1	CS/SiO2					537:543	interconnected porous chitosan-silica (CS/SiO2 ) and chitosan-silica-hydroxyapatite (CS/SiO2 /HA) hybrids	498:602	CS/SiO2	537:543	In this study, we fabricated the three-dimensional (3D) interconnected porous chitosan-silica (CS/SiO2 ) and chitosan-silica-hydroxyapatite (CS/SiO2 /HA) hybrids, through sol-gel process and 3D plotting skill, followed by the naturally or freeze drying separately.
29314593	6	52	theme	CS/SiO2	1128:1134	arg1	hybrid					1136:1141	Naturally dried CS/SiO2 hybrid	1112:1141	Naturally dried CS/SiO2 hybrid	1112:1141	Naturally dried CS/SiO2 hybrid provoked a more severe inflammation than the freeze-dried ones.
29314593	3	53	theme	chitosan-silica	520:534	arg1	CS/SiO2					537:543	interconnected porous chitosan-silica (CS/SiO2 ) and chitosan-silica-hydroxyapatite (CS/SiO2 /HA) hybrids	498:602	CS/SiO2	537:543	In this study, we fabricated the three-dimensional (3D) interconnected porous chitosan-silica (CS/SiO2 ) and chitosan-silica-hydroxyapatite (CS/SiO2 /HA) hybrids, through sol-gel process and 3D plotting skill, followed by the naturally or freeze drying separately.
29314593	0	54	theme	porous	42:47	arg1	chitosan/SiO2					67:79	the porous three-dimensional chitosan/SiO2	38:79	the porous three-dimensional chitosan/SiO2	38:79	In vivo immuno-reactivity analysis of the porous three-dimensional chitosan/SiO2 and chitosan/SiO2 /hydroxyapatite hybrids.
29314593	7	55	theme	cells	1371:1375	arg1	absence					1348:1354	the absence	1344:1354	the absence of cytotoxic T cells and B cells in muscle	1344:1397	Dendritic cells were attracted to invade into the implants embedded-muscle, but not be activated to prime the adaptive immunity, because the absence of cytotoxic T cells and B cells in muscle received the implants.
29314593	4	56	theme	nanoscale	854:862	arg1	silica					864:869	nanoscale silica	854:869	nanoscale silica	854:869	Scanning electron microscopy demonstrated the hybrids possessed the uniform geometric structure, while, transmission electron microscopy displayed nanoscale silica, or HA nanoparticles dispersed homogeneously in the CS matrix, or CS/silica hybrids.
29314593	3	57	theme	interconnected	498:511	arg1	CS/SiO2					537:543	interconnected porous chitosan-silica (CS/SiO2 ) and chitosan-silica-hydroxyapatite (CS/SiO2 /HA) hybrids	498:602	CS/SiO2	537:543	In this study, we fabricated the three-dimensional (3D) interconnected porous chitosan-silica (CS/SiO2 ) and chitosan-silica-hydroxyapatite (CS/SiO2 /HA) hybrids, through sol-gel process and 3D plotting skill, followed by the naturally or freeze drying separately.
29314593	1	58	theme	controllable	240:251	arg1	properties					264:273	the controllable mechanical properties	236:273	the controllable mechanical properties	236:273	Inorganic/organic hybrid silica-chitosan (CS) scaffolds have promising potential for bone defect repair, due to the controllable mechanical properties, degradation behavior, and scaffold morphology.
29314593	10	59	theme	CS/SiO2	1816:1822	arg1	hybrids					1828:1834	3D plotted porous CS/SiO2 and CS/SiO2 /HA hybrids	1786:1834	hybrids	1828:1834	Thus, the present results suggest that, in vivo, 3D plotted porous CS/SiO2 and CS/SiO2 /HA hybrids are relatively biocompatible in vivo, which initiate a localized inflammatory procedure, instead of a systematic immune response.
29314593	9	60	theme	striking	1718:1725	arg1	decrease					1727:1734	the subsequent striking decrease	1703:1734	the subsequent striking decrease	1703:1734	Plasma complement C3 enzyme linked immunosorbent assay (ELISA) assay showed the hybrids induced C3 levels increase in early implanting phase, and the subsequent striking decrease.
29314593	7	61	theme	cytotoxic	1359:1367	arg1	cells					1371:1375	cytotoxic T cells	1359:1375	cytotoxic T cells	1359:1375	Dendritic cells were attracted to invade into the implants embedded-muscle, but not be activated to prime the adaptive immunity, because the absence of cytotoxic T cells and B cells in muscle received the implants.
29314593	7	62	theme	B	1381:1381	arg1	cells					1383:1387	B cells	1381:1387	B cells	1381:1387	Dendritic cells were attracted to invade into the implants embedded-muscle, but not be activated to prime the adaptive immunity, because the absence of cytotoxic T cells and B cells in muscle received the implants.
29314593	5	63	theme	myofiber	1089:1096	arg1	degeneration					1098:1109	myofiber degeneration	1089:1109	myofiber degeneration	1089:1109	After intramuscular implantation, CS/SiO2 and CS/SiO2 /HA hybrids triggered a local and limited monocyte/macrophage infiltration and myofiber degeneration.
29314593	10	64	theme	porous	1797:1802	arg1	CS/SiO2					1804:1810	3D plotted porous CS/SiO2 and CS/SiO2 /HA hybrids	1786:1834	CS/SiO2	1804:1810	Thus, the present results suggest that, in vivo, 3D plotted porous CS/SiO2 and CS/SiO2 /HA hybrids are relatively biocompatible in vivo, which initiate a localized inflammatory procedure, instead of a systematic immune response.
29314593	1	65	theme	degradation	276:286	arg1	behavior					288:295	degradation behavior	276:295	degradation behavior	276:295	Inorganic/organic hybrid silica-chitosan (CS) scaffolds have promising potential for bone defect repair, due to the controllable mechanical properties, degradation behavior, and scaffold morphology.
29314593	7	66	theme	Dendritic	1207:1215	arg1	cells					1217:1221	Dendritic cells	1207:1221	Dendritic cells	1207:1221	Dendritic cells were attracted to invade into the implants embedded-muscle, but not be activated to prime the adaptive immunity, because the absence of cytotoxic T cells and B cells in muscle received the implants.
29314593	3	67	theme	/HA	591:593	arg1	hybrids					596:602	interconnected porous chitosan-silica (CS/SiO2 ) and chitosan-silica-hydroxyapatite (CS/SiO2 /HA) hybrids	498:602	hybrids	596:602	In this study, we fabricated the three-dimensional (3D) interconnected porous chitosan-silica (CS/SiO2 ) and chitosan-silica-hydroxyapatite (CS/SiO2 /HA) hybrids, through sol-gel process and 3D plotting skill, followed by the naturally or freeze drying separately.
29314593	2	68	theme	silica-CS	373:381	arg1	hybrids					383:389	silica-CS hybrids	373:389	silica-CS hybrids	373:389	However, the precise in vivo immuno-reactivity of silica-CS hybrids with various compositions is still poorly defined.
29314593	10	69	theme	/HA	1824:1826	arg1	hybrids					1828:1834	3D plotted porous CS/SiO2 and CS/SiO2 /HA hybrids	1786:1834	hybrids	1828:1834	Thus, the present results suggest that, in vivo, 3D plotted porous CS/SiO2 and CS/SiO2 /HA hybrids are relatively biocompatible in vivo, which initiate a localized inflammatory procedure, instead of a systematic immune response.
29314593	2	70	theme	in	344:345	arg1	immuno-reactivity					352:368	the precise in vivo immuno-reactivity	332:368	the precise in vivo immuno-reactivity of silica-CS hybrids with various compositions	332:415	However, the precise in vivo immuno-reactivity of silica-CS hybrids with various compositions is still poorly defined.
29314593	1	71	theme	defect	214:219	arg1	repair					221:226	bone defect repair	209:226	bone defect repair	209:226	Inorganic/organic hybrid silica-chitosan (CS) scaffolds have promising potential for bone defect repair, due to the controllable mechanical properties, degradation behavior, and scaffold morphology.
29314593	9	72	theme	complement	1564:1573	arg1	assay					1620:1624	Plasma complement C3 enzyme linked immunosorbent assay (ELISA) assay	1557:1624	Plasma complement C3 enzyme linked immunosorbent assay (ELISA) assay	1557:1624	Plasma complement C3 enzyme linked immunosorbent assay (ELISA) assay showed the hybrids induced C3 levels increase in early implanting phase, and the subsequent striking decrease.
29314593	3	73	theme	sol-gel	613:619	arg1	process					621:627	sol-gel process	613:627	sol-gel process	613:627	In this study, we fabricated the three-dimensional (3D) interconnected porous chitosan-silica (CS/SiO2 ) and chitosan-silica-hydroxyapatite (CS/SiO2 /HA) hybrids, through sol-gel process and 3D plotting skill, followed by the naturally or freeze drying separately.
29314593	9	74	theme	enzyme	1578:1583	arg1	assay					1620:1624	Plasma complement C3 enzyme linked immunosorbent assay (ELISA) assay	1557:1624	Plasma complement C3 enzyme linked immunosorbent assay (ELISA) assay	1557:1624	Plasma complement C3 enzyme linked immunosorbent assay (ELISA) assay showed the hybrids induced C3 levels increase in early implanting phase, and the subsequent striking decrease.
29314593	9	75	theme	immunosorbent	1592:1604	arg1	assay					1620:1624	Plasma complement C3 enzyme linked immunosorbent assay (ELISA) assay	1557:1624	Plasma complement C3 enzyme linked immunosorbent assay (ELISA) assay	1557:1624	Plasma complement C3 enzyme linked immunosorbent assay (ELISA) assay showed the hybrids induced C3 levels increase in early implanting phase, and the subsequent striking decrease.
29314593	9	76	theme	subsequent	1707:1716	arg1	decrease					1727:1734	the subsequent striking decrease	1703:1734	the subsequent striking decrease	1703:1734	Plasma complement C3 enzyme linked immunosorbent assay (ELISA) assay showed the hybrids induced C3 levels increase in early implanting phase, and the subsequent striking decrease.
29314593	6	77	theme	dried	1122:1126	arg1	hybrid					1136:1141	Naturally dried CS/SiO2 hybrid	1112:1141	Naturally dried CS/SiO2 hybrid	1112:1141	Naturally dried CS/SiO2 hybrid provoked a more severe inflammation than the freeze-dried ones.
29314593	4	78	theme	transmission	811:822	arg1	microscopy					833:842	transmission electron microscopy	811:842	transmission electron microscopy	811:842	Scanning electron microscopy demonstrated the hybrids possessed the uniform geometric structure, while, transmission electron microscopy displayed nanoscale silica, or HA nanoparticles dispersed homogeneously in the CS matrix, or CS/silica hybrids.
29314593	7	79	theme	adaptive	1317:1324	arg1	immunity					1326:1333	the adaptive immunity	1313:1333	the adaptive immunity	1313:1333	Dendritic cells were attracted to invade into the implants embedded-muscle, but not be activated to prime the adaptive immunity, because the absence of cytotoxic T cells and B cells in muscle received the implants.
29314593	10	80	theme	inflammatory	1901:1912	arg1	procedure					1914:1922	a localized inflammatory procedure	1889:1922	a localized inflammatory procedure	1889:1922	Thus, the present results suggest that, in vivo, 3D plotted porous CS/SiO2 and CS/SiO2 /HA hybrids are relatively biocompatible in vivo, which initiate a localized inflammatory procedure, instead of a systematic immune response.
29314593	4	81	theme	electron	716:723	arg1	microscopy					725:734	Scanning electron microscopy	707:734	Scanning electron microscopy	707:734	Scanning electron microscopy demonstrated the hybrids possessed the uniform geometric structure, while, transmission electron microscopy displayed nanoscale silica, or HA nanoparticles dispersed homogeneously in the CS matrix, or CS/silica hybrids.
29314593	1	82	theme	promising	185:193	arg1	potential					195:203	promising potential	185:203	promising potential for bone defect repair, due to the controllable mechanical properties, degradation behavior, and scaffold morphology	185:320	Inorganic/organic hybrid silica-chitosan (CS) scaffolds have promising potential for bone defect repair, due to the controllable mechanical properties, degradation behavior, and scaffold morphology.
29314593	12	83	dep	Res	2012:2014	arg1	A					2021:2021	Part A	2016:2021	J Biomed Mater Res Part A: 106A: 1223-1235, 2018.	1997:2045	J Biomed Mater Res Part A: 106A: 1223-1235, 2018.
29314593	12	83	dep	Res	2012:2014	arg1	106A					2024:2027	106A	2024:2027	106A	2024:2027	J Biomed Mater Res Part A: 106A: 1223-1235, 2018.
29314593	9	84	theme	C3	1575:1576	arg1	assay					1620:1624	Plasma complement C3 enzyme linked immunosorbent assay (ELISA) assay	1557:1624	Plasma complement C3 enzyme linked immunosorbent assay (ELISA) assay	1557:1624	Plasma complement C3 enzyme linked immunosorbent assay (ELISA) assay showed the hybrids induced C3 levels increase in early implanting phase, and the subsequent striking decrease.
29314593	9	85	theme	C3	1653:1654	arg1	levels					1656:1661	C3 levels	1653:1661	C3 levels increase in early implanting phase	1653:1696	Plasma complement C3 enzyme linked immunosorbent assay (ELISA) assay showed the hybrids induced C3 levels increase in early implanting phase, and the subsequent striking decrease.
29314593	8	86	theme	cell	1445:1448	arg1	FACS					1459:1462	FACS	1459:1462	FACS	1459:1462	Fluorescence-activated cell sorting (FACS) analysis indicated the implanted hybrids were incapable to initiate splenocytes activation.
29314593	8	86	theme	cell	1445:1448	arg1	sorting					1450:1456	Fluorescence-activated cell sorting	1422:1456	Fluorescence-activated cell sorting (FACS) analysis	1422:1472	Fluorescence-activated cell sorting (FACS) analysis indicated the implanted hybrids were incapable to initiate splenocytes activation.
29314593	5	87	theme	CS/SiO2	1002:1008	arg1	hybrids					1014:1020	CS/SiO2 /HA hybrids	1002:1020	CS/SiO2 /HA hybrids	1002:1020	After intramuscular implantation, CS/SiO2 and CS/SiO2 /HA hybrids triggered a local and limited monocyte/macrophage infiltration and myofiber degeneration.
29314593	6	88	theme	severe	1159:1164	arg1	inflammation					1166:1177	a more severe inflammation	1152:1177	a more severe inflammation than the freeze-dried ones	1152:1204	Naturally dried CS/SiO2 hybrid provoked a more severe inflammation than the freeze-dried ones.
29314593	10	89	theme	immune	1949:1954	arg1	response					1956:1963	a systematic immune response	1936:1963	a systematic immune response	1936:1963	Thus, the present results suggest that, in vivo, 3D plotted porous CS/SiO2 and CS/SiO2 /HA hybrids are relatively biocompatible in vivo, which initiate a localized inflammatory procedure, instead of a systematic immune response.
29314593	4	90	theme	geometric	783:791	arg1	structure					793:801	the uniform geometric structure	771:801	the uniform geometric structure	771:801	Scanning electron microscopy demonstrated the hybrids possessed the uniform geometric structure, while, transmission electron microscopy displayed nanoscale silica, or HA nanoparticles dispersed homogeneously in the CS matrix, or CS/silica hybrids.
29314593	1	91	theme	Inorganic/organic	124:140	arg1	CS					166:167	CS	166:167	CS	166:167	Inorganic/organic hybrid silica-chitosan (CS) scaffolds have promising potential for bone defect repair, due to the controllable mechanical properties, degradation behavior, and scaffold morphology.
29314593	1	91	theme	Inorganic/organic	124:140	arg1	silica-chitosan					149:163	Inorganic/organic hybrid silica-chitosan	124:163	Inorganic/organic hybrid silica-chitosan (CS) scaffolds	124:178	Inorganic/organic hybrid silica-chitosan (CS) scaffolds have promising potential for bone defect repair, due to the controllable mechanical properties, degradation behavior, and scaffold morphology.
29314593	7	92	theme	cells	1383:1387	arg1	absence					1348:1354	the absence	1344:1354	the absence of cytotoxic T cells and B cells in muscle	1344:1397	Dendritic cells were attracted to invade into the implants embedded-muscle, but not be activated to prime the adaptive immunity, because the absence of cytotoxic T cells and B cells in muscle received the implants.
29314593	10	93	theme	systematic	1938:1947	arg1	response					1956:1963	a systematic immune response	1936:1963	a systematic immune response	1936:1963	Thus, the present results suggest that, in vivo, 3D plotted porous CS/SiO2 and CS/SiO2 /HA hybrids are relatively biocompatible in vivo, which initiate a localized inflammatory procedure, instead of a systematic immune response.
29314593	0	94	theme	three-dimensional	49:65	arg1	chitosan/SiO2					67:79	the porous three-dimensional chitosan/SiO2	38:79	the porous three-dimensional chitosan/SiO2	38:79	In vivo immuno-reactivity analysis of the porous three-dimensional chitosan/SiO2 and chitosan/SiO2 /hydroxyapatite hybrids.
29314593	7	95	theme	T	1369:1369	arg1	cells					1371:1375	cytotoxic T cells	1359:1375	cytotoxic T cells	1359:1375	Dendritic cells were attracted to invade into the implants embedded-muscle, but not be activated to prime the adaptive immunity, because the absence of cytotoxic T cells and B cells in muscle received the implants.
29314593	2	96	theme	various	396:402	arg1	compositions					404:415	various compositions	396:415	various compositions	396:415	However, the precise in vivo immuno-reactivity of silica-CS hybrids with various compositions is still poorly defined.
29314593	8	97	theme	implanted	1488:1496	arg1	incapable					1511:1519	incapable	1511:1519	incapable	1511:1519	Fluorescence-activated cell sorting (FACS) analysis indicated the implanted hybrids were incapable to initiate splenocytes activation.
29314593	8	97	theme	implanted	1488:1496	arg1	hybrids					1498:1504	the implanted hybrids	1484:1504	the implanted hybrids	1484:1504	Fluorescence-activated cell sorting (FACS) analysis indicated the implanted hybrids were incapable to initiate splenocytes activation.
29314593	0	98	dep	In	0:1	arg1	vivo					3:6	vivo	3:6	vivo	3:6	In vivo immuno-reactivity analysis of the porous three-dimensional chitosan/SiO2 and chitosan/SiO2 /hydroxyapatite hybrids.
29314593	5	99	theme	monocyte/macrophage	1052:1070	arg1	infiltration					1072:1083	a local and limited monocyte/macrophage infiltration	1032:1083	a local and limited monocyte/macrophage infiltration	1032:1083	After intramuscular implantation, CS/SiO2 and CS/SiO2 /HA hybrids triggered a local and limited monocyte/macrophage infiltration and myofiber degeneration.
29314593	9	100	theme	levels	1656:1661	arg1	increase					1663:1670	C3 levels increase	1653:1670	C3 levels increase in early implanting phase	1653:1696	Plasma complement C3 enzyme linked immunosorbent assay (ELISA) assay showed the hybrids induced C3 levels increase in early implanting phase, and the subsequent striking decrease.
29314593	3	101	theme	porous	513:518	arg1	CS/SiO2					537:543	interconnected porous chitosan-silica (CS/SiO2 ) and chitosan-silica-hydroxyapatite (CS/SiO2 /HA) hybrids	498:602	CS/SiO2	537:543	In this study, we fabricated the three-dimensional (3D) interconnected porous chitosan-silica (CS/SiO2 ) and chitosan-silica-hydroxyapatite (CS/SiO2 /HA) hybrids, through sol-gel process and 3D plotting skill, followed by the naturally or freeze drying separately.
29314593	1	102	theme	mechanical	253:262	arg1	properties					264:273	the controllable mechanical properties	236:273	the controllable mechanical properties	236:273	Inorganic/organic hybrid silica-chitosan (CS) scaffolds have promising potential for bone defect repair, due to the controllable mechanical properties, degradation behavior, and scaffold morphology.
29314593	3	103	theme	chitosan-silica-hydroxyapatite	551:580	arg1	hybrids					596:602	interconnected porous chitosan-silica (CS/SiO2 ) and chitosan-silica-hydroxyapatite (CS/SiO2 /HA) hybrids	498:602	hybrids	596:602	In this study, we fabricated the three-dimensional (3D) interconnected porous chitosan-silica (CS/SiO2 ) and chitosan-silica-hydroxyapatite (CS/SiO2 /HA) hybrids, through sol-gel process and 3D plotting skill, followed by the naturally or freeze drying separately.
31647398	2	0	theme	Gram-stain-negative	102:120	arg1	bacterium					167:175	A Gram-stain-negative, non-motile, strictly aerobic, coccus-shaped bacterium	100:175	A Gram-stain-negative, non-motile, strictly aerobic, coccus-shaped bacterium	100:175	A Gram-stain-negative, non-motile, strictly aerobic, coccus-shaped bacterium, designated S14-83T, was isolated from a soil sample collected from the South Shetland Islands of Antarctica.
31647398	8	1	theme	Strain	950:955	arg1	S14-83T					957:963	Strain S14-83T	950:963	Strain S14-83T	950:963	Strain S14-83T was shown to be extremely resistant to gamma radiation (>10 kGy) and UV light (460 Jm-2).
31647398	3	2	theme	%	473:473	arg1	similarity					475:484	96.1 % similarity	468:484	96.1 % similarity	468:484	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain is a novel member of the genus Deinococcus, with Deinococcus alpinitundrae as its closest relative (96.1 % similarity).
31647398	3	2	theme	%	473:473	arg1	relative					458:465	its closest relative	446:465	its closest relative (96.1 % similarity)	446:485	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain is a novel member of the genus Deinococcus, with Deinococcus alpinitundrae as its closest relative (96.1 % similarity).
31647398	9	3	dep	Deinococcus	1223:1233	arg1	psychrotolerans					1235:1249	psychrotolerans	1235:1249	psychrotolerans	1235:1249	On the basis of phylogenetic, chemotaxonomic and phenotypic data presented here, strain S14-83T represents a novel species of the genus Deinococcus, for which the name Deinococcus psychrotolerans sp.
31647398	6	4	theme	major	744:748	arg1	glycolipid					730:739	glycolipid	730:739	glycolipid	730:739	As well as containing glycophospholipid, aminophospholipids and glycolipid as major polar lipids, there were also some unknown polar lipids.
31647398	6	4	theme	major	744:748	arg1	aminophospholipids					707:724	aminophospholipids	707:724	aminophospholipids	707:724	As well as containing glycophospholipid, aminophospholipids and glycolipid as major polar lipids, there were also some unknown polar lipids.
31647398	6	4	theme	major	744:748	arg1	lipids					756:761	major polar lipids	744:761	major polar lipids	744:761	As well as containing glycophospholipid, aminophospholipids and glycolipid as major polar lipids, there were also some unknown polar lipids.
31647398	6	4	theme	major	744:748	arg1	glycophospholipid					688:704	glycophospholipid	688:704	glycophospholipid	688:704	As well as containing glycophospholipid, aminophospholipids and glycolipid as major polar lipids, there were also some unknown polar lipids.
31647398	5	5	dep	C16 	630:633	arg1	 1ω6c					646:650	 1ω6c	646:650	 1ω6c	646:650	Major cellular fatty acids were summed feature 3 (C16 : 1ω7c/C16 : 1ω6c) and C16 : 0.
31647398	5	5	dep	C16 	630:633	arg1	 1ω7c/C16 					635:644	 1ω7c/C16 	635:644	 1ω7c/C16 	635:644	Major cellular fatty acids were summed feature 3 (C16 : 1ω7c/C16 : 1ω6c) and C16 : 0.
31647398	5	6	theme	summed	612:617	arg1	feature					619:625	summed feature 3	612:627	summed feature 3 (C16 : 1ω7c/C16 : 1ω6c)	612:651	Major cellular fatty acids were summed feature 3 (C16 : 1ω7c/C16 : 1ω6c) and C16 : 0.
31647398	2	7	attach	isolated	202:209	arg1	sample					223:228	a soil sample	216:228	a soil sample collected from the South Shetland Islands of Antarctica	216:284	A Gram-stain-negative, non-motile, strictly aerobic, coccus-shaped bacterium, designated S14-83T, was isolated from a soil sample collected from the South Shetland Islands of Antarctica.
31647398	2	7	attach	isolated	202:209	arg2	bacterium					167:175	A Gram-stain-negative, non-motile, strictly aerobic, coccus-shaped bacterium	100:175	A Gram-stain-negative, non-motile, strictly aerobic, coccus-shaped bacterium	100:175	A Gram-stain-negative, non-motile, strictly aerobic, coccus-shaped bacterium, designated S14-83T, was isolated from a soil sample collected from the South Shetland Islands of Antarctica.
31647398	9	8	theme	genus	1185:1189	arg1	Deinococcus					1191:1201	the genus Deinococcus	1181:1201	the genus Deinococcus	1181:1201	On the basis of phylogenetic, chemotaxonomic and phenotypic data presented here, strain S14-83T represents a novel species of the genus Deinococcus, for which the name Deinococcus psychrotolerans sp.
31647398	3	9	with	member	379:384	arg1	alpinitundrae					429:441	Deinococcus alpinitundrae	417:441	Deinococcus alpinitundrae	417:441	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain is a novel member of the genus Deinococcus, with Deinococcus alpinitundrae as its closest relative (96.1 % similarity).
31647398	11	10	theme	2015449T=	1311:1319	arg1	T					1332:1332	=CCTCC AB 2015449T= DSM 105285 T	1301:1332	=CCTCC AB 2015449T= DSM 105285 T	1301:1332	The type strain is S14-83T (=CCTCC AB 2015449T= DSM 105285 T).
31647398	11	10	theme	2015449T=	1311:1319	arg1	S14-83T					1292:1298	S14-83T	1292:1298	S14-83T (=CCTCC AB 2015449T= DSM 105285 T)	1292:1333	The type strain is S14-83T (=CCTCC AB 2015449T= DSM 105285 T).
31647398	3	11	theme	gene	327:330	arg1	sequences					332:340	16S rRNA gene sequences	318:340	16S rRNA gene sequences	318:340	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain is a novel member of the genus Deinococcus, with Deinococcus alpinitundrae as its closest relative (96.1 % similarity).
31647398	9	12	theme	Deinococcus	1191:1201	arg1	species					1170:1176	a novel species	1162:1176	a novel species	1162:1176	On the basis of phylogenetic, chemotaxonomic and phenotypic data presented here, strain S14-83T represents a novel species of the genus Deinococcus, for which the name Deinococcus psychrotolerans sp.
31647398	9	13	theme	phylogenetic	1071:1082	arg1	data					1115:1118	phylogenetic, chemotaxonomic and phenotypic data	1071:1118	phylogenetic, chemotaxonomic and phenotypic data presented here	1071:1133	On the basis of phylogenetic, chemotaxonomic and phenotypic data presented here, strain S14-83T represents a novel species of the genus Deinococcus, for which the name Deinococcus psychrotolerans sp.
31647398	11	14	theme	DSM	1321:1323	arg1	T					1332:1332	=CCTCC AB 2015449T= DSM 105285 T	1301:1332	=CCTCC AB 2015449T= DSM 105285 T	1301:1332	The type strain is S14-83T (=CCTCC AB 2015449T= DSM 105285 T).
31647398	11	14	theme	DSM	1321:1323	arg1	S14-83T					1292:1298	S14-83T	1292:1298	S14-83T (=CCTCC AB 2015449T= DSM 105285 T)	1292:1333	The type strain is S14-83T (=CCTCC AB 2015449T= DSM 105285 T).
31647398	5	15	theme	Major	580:584	arg1	acids					601:605	Major cellular fatty acids	580:605	Major cellular fatty acids	580:605	Major cellular fatty acids were summed feature 3 (C16 : 1ω7c/C16 : 1ω6c) and C16 : 0.
31647398	5	16	dep	feature	619:625	arg1	C16 					630:633	C16 	630:633	C16 	630:633	Major cellular fatty acids were summed feature 3 (C16 : 1ω7c/C16 : 1ω6c) and C16 : 0.
31647398	11	17	theme	type	1277:1280	arg1	strain					1282:1287	The type strain	1273:1287	The type strain	1273:1287	The type strain is S14-83T (=CCTCC AB 2015449T= DSM 105285 T).
31647398	11	17	theme	type	1277:1280	arg1	S14-83T					1292:1298	S14-83T	1292:1298	S14-83T (=CCTCC AB 2015449T= DSM 105285 T)	1292:1333	The type strain is S14-83T (=CCTCC AB 2015449T= DSM 105285 T).
31647398	6	18	theme	polar	793:797	arg1	lipids					799:804	some unknown polar lipids	780:804	some unknown polar lipids	780:804	As well as containing glycophospholipid, aminophospholipids and glycolipid as major polar lipids, there were also some unknown polar lipids.
31647398	5	19	theme	cellular	586:593	arg1	acids					601:605	Major cellular fatty acids	580:605	Major cellular fatty acids	580:605	Major cellular fatty acids were summed feature 3 (C16 : 1ω7c/C16 : 1ω6c) and C16 : 0.
31647398	9	20	theme	chemotaxonomic	1085:1098	arg1	data					1115:1118	phylogenetic, chemotaxonomic and phenotypic data	1071:1118	phylogenetic, chemotaxonomic and phenotypic data presented here	1071:1133	On the basis of phylogenetic, chemotaxonomic and phenotypic data presented here, strain S14-83T represents a novel species of the genus Deinococcus, for which the name Deinococcus psychrotolerans sp.
31647398	11	21	theme	=CCTCC AB	1301:1309	arg1	T					1332:1332	=CCTCC AB 2015449T= DSM 105285 T	1301:1332	=CCTCC AB 2015449T= DSM 105285 T	1301:1332	The type strain is S14-83T (=CCTCC AB 2015449T= DSM 105285 T).
31647398	11	21	theme	=CCTCC AB	1301:1309	arg1	S14-83T					1292:1298	S14-83T	1292:1298	S14-83T (=CCTCC AB 2015449T= DSM 105285 T)	1292:1333	The type strain is S14-83T (=CCTCC AB 2015449T= DSM 105285 T).
31647398	3	22	theme	genus	393:397	arg1	Deinococcus					399:409	the genus Deinococcus	389:409	the genus Deinococcus	389:409	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain is a novel member of the genus Deinococcus, with Deinococcus alpinitundrae as its closest relative (96.1 % similarity).
31647398	0	23	theme	Deinococcus	0:10	arg1	psychrotolerans					12:26	Deinococcus psychrotolerans	0:26	Deinococcus psychrotolerans	0:26	Deinococcus psychrotolerans sp.
31647398	4	24	theme	strain	515:520	arg1	G+C content					496:506	The DNA G+C content	488:506	The DNA G+C content of the strain	488:520	The DNA G+C content of the strain was 61.1 mol% and the major respiratory quinone was MK-8.
31647398	4	24	theme	strain	515:520	arg1	%					534:534	61.1 mol%	526:534	61.1 mol%	526:534	The DNA G+C content of the strain was 61.1 mol% and the major respiratory quinone was MK-8.
31647398	5	25	theme	fatty	595:599	arg1	acids					601:605	Major cellular fatty acids	580:605	Major cellular fatty acids	580:605	Major cellular fatty acids were summed feature 3 (C16 : 1ω7c/C16 : 1ω6c) and C16 : 0.
31647398	9	26	theme	strain	1136:1141	arg1	S14-83T					1143:1149	strain S14-83T	1136:1149	strain S14-83T	1136:1149	On the basis of phylogenetic, chemotaxonomic and phenotypic data presented here, strain S14-83T represents a novel species of the genus Deinococcus, for which the name Deinococcus psychrotolerans sp.
31647398	3	27	theme	Deinococcus	399:409	arg1	member					379:384	a novel member	371:384	a novel member of the genus Deinococcus	371:409	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain is a novel member of the genus Deinococcus, with Deinococcus alpinitundrae as its closest relative (96.1 % similarity).
31647398	3	27	theme	Deinococcus	399:409	arg1	strain					361:366	the strain	357:366	the strain	357:366	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain is a novel member of the genus Deinococcus, with Deinococcus alpinitundrae as its closest relative (96.1 % similarity).
31647398	7	28	theme	cell-wall	842:850	arg1	peptidoglycan					852:864	the cell-wall peptidoglycan	838:864	the cell-wall peptidoglycan	838:864	The diagnostic diamino acid in the cell-wall peptidoglycan was ornithine, corroborating the assignment of the strain to the genus Deinococcus.
31647398	7	29	from	acid	830:833	arg1	peptidoglycan					852:864	the cell-wall peptidoglycan	838:864	the cell-wall peptidoglycan	838:864	The diagnostic diamino acid in the cell-wall peptidoglycan was ornithine, corroborating the assignment of the strain to the genus Deinococcus.
31647398	4	30	theme	61.1 mol	526:533	arg1	G+C content					496:506	The DNA G+C content	488:506	The DNA G+C content of the strain	488:520	The DNA G+C content of the strain was 61.1 mol% and the major respiratory quinone was MK-8.
31647398	4	30	theme	61.1 mol	526:533	arg1	%					534:534	61.1 mol%	526:534	61.1 mol%	526:534	The DNA G+C content of the strain was 61.1 mol% and the major respiratory quinone was MK-8.
31647398	6	31	theme	unknown	785:791	arg1	lipids					799:804	some unknown polar lipids	780:804	some unknown polar lipids	780:804	As well as containing glycophospholipid, aminophospholipids and glycolipid as major polar lipids, there were also some unknown polar lipids.
31647398	11	32	theme	105285	1325:1330	arg1	T					1332:1332	=CCTCC AB 2015449T= DSM 105285 T	1301:1332	=CCTCC AB 2015449T= DSM 105285 T	1301:1332	The type strain is S14-83T (=CCTCC AB 2015449T= DSM 105285 T).
31647398	11	32	theme	105285	1325:1330	arg1	S14-83T					1292:1298	S14-83T	1292:1298	S14-83T (=CCTCC AB 2015449T= DSM 105285 T)	1292:1333	The type strain is S14-83T (=CCTCC AB 2015449T= DSM 105285 T).
31647398	9	33	theme	Deinococcus	1223:1233	arg1	sp					1251:1252	the name Deinococcus psychrotolerans sp	1214:1252	the name Deinococcus psychrotolerans sp	1214:1252	On the basis of phylogenetic, chemotaxonomic and phenotypic data presented here, strain S14-83T represents a novel species of the genus Deinococcus, for which the name Deinococcus psychrotolerans sp.
31647398	2	34	theme	soil	218:221	arg1	sample					223:228	a soil sample	216:228	a soil sample collected from the South Shetland Islands of Antarctica	216:284	A Gram-stain-negative, non-motile, strictly aerobic, coccus-shaped bacterium, designated S14-83T, was isolated from a soil sample collected from the South Shetland Islands of Antarctica.
31647398	9	35	theme	name	1218:1221	arg1	sp					1251:1252	the name Deinococcus psychrotolerans sp	1214:1252	the name Deinococcus psychrotolerans sp	1214:1252	On the basis of phylogenetic, chemotaxonomic and phenotypic data presented here, strain S14-83T represents a novel species of the genus Deinococcus, for which the name Deinococcus psychrotolerans sp.
31647398	4	36	theme	DNA	492:494	arg1	G+C content					496:506	The DNA G+C content	488:506	The DNA G+C content of the strain	488:520	The DNA G+C content of the strain was 61.1 mol% and the major respiratory quinone was MK-8.
31647398	4	36	theme	DNA	492:494	arg1	%					534:534	61.1 mol%	526:534	61.1 mol%	526:534	The DNA G+C content of the strain was 61.1 mol% and the major respiratory quinone was MK-8.
31647398	8	37	theme	gamma	1004:1008	arg1	>10 kGy					1021:1027	>10 kGy	1021:1027	>10 kGy	1021:1027	Strain S14-83T was shown to be extremely resistant to gamma radiation (>10 kGy) and UV light (460 Jm-2).
31647398	8	37	theme	gamma	1004:1008	arg1	radiation					1010:1018	gamma radiation	1004:1018	gamma radiation (>10 kGy)	1004:1028	Strain S14-83T was shown to be extremely resistant to gamma radiation (>10 kGy) and UV light (460 Jm-2).
31647398	7	38	theme	genus	931:935	arg1	Deinococcus					937:947	the genus Deinococcus	927:947	the genus Deinococcus	927:947	The diagnostic diamino acid in the cell-wall peptidoglycan was ornithine, corroborating the assignment of the strain to the genus Deinococcus.
31647398	3	39	theme	novel	373:377	arg1	member					379:384	a novel member	371:384	a novel member of the genus Deinococcus	371:409	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain is a novel member of the genus Deinococcus, with Deinococcus alpinitundrae as its closest relative (96.1 % similarity).
31647398	3	39	theme	novel	373:377	arg1	strain					361:366	the strain	357:366	the strain	357:366	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain is a novel member of the genus Deinococcus, with Deinococcus alpinitundrae as its closest relative (96.1 % similarity).
31647398	3	40	theme	Phylogenetic	287:298	arg1	analysis					300:307	Phylogenetic analysis	287:307	Phylogenetic analysis based on 16S rRNA gene sequences	287:340	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain is a novel member of the genus Deinococcus, with Deinococcus alpinitundrae as its closest relative (96.1 % similarity).
31647398	8	41	theme	UV	1034:1035	arg1	light					1037:1041	UV light	1034:1041	UV light (460 Jm-2)	1034:1052	Strain S14-83T was shown to be extremely resistant to gamma radiation (>10 kGy) and UV light (460 Jm-2).
31647398	8	41	theme	UV	1034:1035	arg1	Jm-2					1048:1051	460 Jm-2	1044:1051	460 Jm-2	1044:1051	Strain S14-83T was shown to be extremely resistant to gamma radiation (>10 kGy) and UV light (460 Jm-2).
31647398	9	42	theme	phenotypic	1104:1113	arg1	data					1115:1118	phylogenetic, chemotaxonomic and phenotypic data	1071:1118	phylogenetic, chemotaxonomic and phenotypic data presented here	1071:1133	On the basis of phylogenetic, chemotaxonomic and phenotypic data presented here, strain S14-83T represents a novel species of the genus Deinococcus, for which the name Deinococcus psychrotolerans sp.
31647398	4	43	theme	major	544:548	arg1	quinone					562:568	the major respiratory quinone	540:568	the major respiratory quinone	540:568	The DNA G+C content of the strain was 61.1 mol% and the major respiratory quinone was MK-8.
31647398	4	43	theme	major	544:548	arg1	MK-8					574:577	MK-8	574:577	MK-8	574:577	The DNA G+C content of the strain was 61.1 mol% and the major respiratory quinone was MK-8.
31647398	3	44	theme	96.1 	468:472	arg1	%					473:473	%	473:473	%	473:473	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain is a novel member of the genus Deinococcus, with Deinococcus alpinitundrae as its closest relative (96.1 % similarity).
31647398	2	45	dep	Gram-stain-negative	102:120	arg1	coccus-shaped					153:165	coccus-shaped	153:165	coccus-shaped	153:165	A Gram-stain-negative, non-motile, strictly aerobic, coccus-shaped bacterium, designated S14-83T, was isolated from a soil sample collected from the South Shetland Islands of Antarctica.
31647398	2	45	dep	Gram-stain-negative	102:120	arg1	non-motile					123:132	non-motile	123:132	non-motile	123:132	A Gram-stain-negative, non-motile, strictly aerobic, coccus-shaped bacterium, designated S14-83T, was isolated from a soil sample collected from the South Shetland Islands of Antarctica.
31647398	2	45	dep	Gram-stain-negative	102:120	arg1	aerobic					144:150	aerobic	144:150	aerobic	144:150	A Gram-stain-negative, non-motile, strictly aerobic, coccus-shaped bacterium, designated S14-83T, was isolated from a soil sample collected from the South Shetland Islands of Antarctica.
31647398	3	46	theme	closest	450:456	arg1	similarity					475:484	96.1 % similarity	468:484	96.1 % similarity	468:484	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain is a novel member of the genus Deinococcus, with Deinococcus alpinitundrae as its closest relative (96.1 % similarity).
31647398	3	46	theme	closest	450:456	arg1	relative					458:465	its closest relative	446:465	its closest relative (96.1 % similarity)	446:485	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain is a novel member of the genus Deinococcus, with Deinococcus alpinitundrae as its closest relative (96.1 % similarity).
31647398	0	47	dep	sp	28:29	arg1	psychrotolerans					12:26	Deinococcus psychrotolerans	0:26	Deinococcus psychrotolerans	0:26	Deinococcus psychrotolerans sp.
31647398	7	48	theme	diagnostic	811:820	arg1	acid					830:833	The diagnostic diamino acid	807:833	The diagnostic diamino acid in the cell-wall peptidoglycan	807:864	The diagnostic diamino acid in the cell-wall peptidoglycan was ornithine, corroborating the assignment of the strain to the genus Deinococcus.
31647398	7	48	theme	diagnostic	811:820	arg1	ornithine					870:878	ornithine	870:878	ornithine	870:878	The diagnostic diamino acid in the cell-wall peptidoglycan was ornithine, corroborating the assignment of the strain to the genus Deinococcus.
31647398	4	49	theme	respiratory	550:560	arg1	quinone					562:568	the major respiratory quinone	540:568	the major respiratory quinone	540:568	The DNA G+C content of the strain was 61.1 mol% and the major respiratory quinone was MK-8.
31647398	4	49	theme	respiratory	550:560	arg1	MK-8					574:577	MK-8	574:577	MK-8	574:577	The DNA G+C content of the strain was 61.1 mol% and the major respiratory quinone was MK-8.
31647398	3	50	theme	16S	318:320	arg1	sequences					332:340	16S rRNA gene sequences	318:340	16S rRNA gene sequences	318:340	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain is a novel member of the genus Deinococcus, with Deinococcus alpinitundrae as its closest relative (96.1 % similarity).
31647398	6	51	theme	polar	750:754	arg1	glycolipid					730:739	glycolipid	730:739	glycolipid	730:739	As well as containing glycophospholipid, aminophospholipids and glycolipid as major polar lipids, there were also some unknown polar lipids.
31647398	6	51	theme	polar	750:754	arg1	aminophospholipids					707:724	aminophospholipids	707:724	aminophospholipids	707:724	As well as containing glycophospholipid, aminophospholipids and glycolipid as major polar lipids, there were also some unknown polar lipids.
31647398	6	51	theme	polar	750:754	arg1	lipids					756:761	major polar lipids	744:761	major polar lipids	744:761	As well as containing glycophospholipid, aminophospholipids and glycolipid as major polar lipids, there were also some unknown polar lipids.
31647398	6	51	theme	polar	750:754	arg1	glycophospholipid					688:704	glycophospholipid	688:704	glycophospholipid	688:704	As well as containing glycophospholipid, aminophospholipids and glycolipid as major polar lipids, there were also some unknown polar lipids.
31647398	7	52	theme	strain	917:922	arg1	assignment					899:908	the assignment	895:908	the assignment of the strain to the genus Deinococcus	895:947	The diagnostic diamino acid in the cell-wall peptidoglycan was ornithine, corroborating the assignment of the strain to the genus Deinococcus.
31647398	7	53	theme	diamino	822:828	arg1	acid					830:833	The diagnostic diamino acid	807:833	The diagnostic diamino acid in the cell-wall peptidoglycan	807:864	The diagnostic diamino acid in the cell-wall peptidoglycan was ornithine, corroborating the assignment of the strain to the genus Deinococcus.
31647398	7	53	theme	diamino	822:828	arg1	ornithine					870:878	ornithine	870:878	ornithine	870:878	The diagnostic diamino acid in the cell-wall peptidoglycan was ornithine, corroborating the assignment of the strain to the genus Deinococcus.
31647398	9	54	dep	data	1115:1118	arg1	the					1058:1060	the	1058:1060	the	1058:1060	On the basis of phylogenetic, chemotaxonomic and phenotypic data presented here, strain S14-83T represents a novel species of the genus Deinococcus, for which the name Deinococcus psychrotolerans sp.
31647398	9	54	dep	data	1115:1118	arg1	basis					1062:1066	basis	1062:1066	basis	1062:1066	On the basis of phylogenetic, chemotaxonomic and phenotypic data presented here, strain S14-83T represents a novel species of the genus Deinococcus, for which the name Deinococcus psychrotolerans sp.
31647398	3	55	theme	rRNA	322:325	arg1	sequences					332:340	16S rRNA gene sequences	318:340	16S rRNA gene sequences	318:340	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain is a novel member of the genus Deinococcus, with Deinococcus alpinitundrae as its closest relative (96.1 % similarity).
31647398	9	56	theme	novel	1164:1168	arg1	species					1170:1176	a novel species	1162:1176	a novel species	1162:1176	On the basis of phylogenetic, chemotaxonomic and phenotypic data presented here, strain S14-83T represents a novel species of the genus Deinococcus, for which the name Deinococcus psychrotolerans sp.
29450536	8	0	theme	Nitrate/nitrite	1346:1360	arg1	levels					1362:1367	Nitrate/nitrite levels	1346:1367	Nitrate/nitrite levels in HLE cells	1346:1380	Nitrate/nitrite levels in HLE cells showed a significant increase with 2% Cu-Ch treatment compared to CS.
29450536	6	1	theme	HCE	1093:1095	arg1	cells					1097:1101	HCE cells	1093:1101	HCE cells treated with Cu-Ch (1% and 2%)	1093:1132	Results Wound closure significantly increased in HCE cells treated with Cu-Ch (1% and 2%) after 72 hours, while HLE cells showed a significant decrease in closure with Cu-Ch (1% and 2%) treatment compared to CS.
29450536	8	2	theme	2	1417:1417	arg1	%					1418:1418	%	1418:1418	%	1418:1418	Nitrate/nitrite levels in HLE cells showed a significant increase with 2% Cu-Ch treatment compared to CS.
29450536	1	3	theme	cell	234:237	arg1	migration					239:247	cell migration	234:247	cell migration	234:247	Purpose Nitric oxide (NO) has gained attention for its role in facilitating wound healing by promoting cell migration, while being cytoprotective in a variety of cell types.
29450536	4	4	theme	total	872:876	arg1	concentrations					894:907	total nitrate/nitrite concentrations	872:907	total nitrate/nitrite concentrations	872:907	Cell migration, cytotoxicity, apoptosis, and total nitrate/nitrite concentrations were measured at 24, 48, and 72 hours after injury and treatment.
29450536	1	5	from	variety	282:288	arg1	cytoprotective					262:275	cytoprotective	262:275	cytoprotective	262:275	Purpose Nitric oxide (NO) has gained attention for its role in facilitating wound healing by promoting cell migration, while being cytoprotective in a variety of cell types.
29450536	8	6	theme	Cu-Ch	1420:1424	arg1	treatment					1426:1434	2% Cu-Ch treatment	1417:1434	2% Cu-Ch treatment	1417:1434	Nitrate/nitrite levels in HLE cells showed a significant increase with 2% Cu-Ch treatment compared to CS.
29450536	3	7	dep	Methods	547:553	arg1	monitored					631:639	monitored	631:639	were monitored under no-scratch (CON), untreated scratch (CS), scratch + plain chitosan composite (0%), scratch + 1% copper solution Cu-Ch (1%), and scratch + 2% copper solution Cu-Ch (2%) conditions	626:824	Methods Human corneal epithelial (HCE) and human limbal epithelial (HLE) cells were monitored under no-scratch (CON), untreated scratch (CS), scratch + plain chitosan composite (0%), scratch + 1% copper solution Cu-Ch (1%), and scratch + 2% copper solution Cu-Ch (2%) conditions.
29450536	8	8	from	levels	1362:1367	arg1	cells					1376:1380	HLE cells	1372:1380	HLE cells	1372:1380	Nitrate/nitrite levels in HLE cells showed a significant increase with 2% Cu-Ch treatment compared to CS.
29450536	6	9	theme	Results	1044:1050	arg1	closure					1058:1064	Results Wound closure	1044:1064	Results Wound closure	1044:1064	Results Wound closure significantly increased in HCE cells treated with Cu-Ch (1% and 2%) after 72 hours, while HLE cells showed a significant decrease in closure with Cu-Ch (1% and 2%) treatment compared to CS.
29450536	3	10	theme	untreated	665:673	arg1	CS					684:685	CS	684:685	CS	684:685	Methods Human corneal epithelial (HCE) and human limbal epithelial (HLE) cells were monitored under no-scratch (CON), untreated scratch (CS), scratch + plain chitosan composite (0%), scratch + 1% copper solution Cu-Ch (1%), and scratch + 2% copper solution Cu-Ch (2%) conditions.
29450536	3	10	theme	untreated	665:673	arg1	scratch					675:681	untreated scratch	665:681	untreated scratch (CS)	665:686	Methods Human corneal epithelial (HCE) and human limbal epithelial (HLE) cells were monitored under no-scratch (CON), untreated scratch (CS), scratch + plain chitosan composite (0%), scratch + 1% copper solution Cu-Ch (1%), and scratch + 2% copper solution Cu-Ch (2%) conditions.
29450536	0	11	theme	Cell	118:121	arg1	Injury					123:128	Human Corneal and Limbal Epithelial Cell Injury	82:128	Injury	123:128	Administration of Nitric Oxide Through a Novel Copper-Chitosan Delivery System in Human Corneal and Limbal Epithelial Cell Injury.
29450536	11	12	from	differences	1719:1729	arg1	production					1737:1746	NO production	1734:1746	NO production	1734:1746	Differences between HCE and HLE responses may be due to intrinsic differences in NO metabolism, as evidenced by differences in NO production, potentially caused by differences in iNOS expression with treatment.
29450536	3	13	theme	scratch	689:695	arg1	conditions					815:824	no-scratch (CON), untreated scratch (CS), scratch + plain chitosan composite (0%), scratch + 1% copper solution Cu-Ch (1%), and scratch + 2% copper solution Cu-Ch (2%) conditions	647:824	no-scratch (CON), untreated scratch (CS), scratch + plain chitosan composite (0%), scratch + 1% copper solution Cu-Ch (1%), and scratch + 2% copper solution Cu-Ch (2%) conditions	647:824	Methods Human corneal epithelial (HCE) and human limbal epithelial (HLE) cells were monitored under no-scratch (CON), untreated scratch (CS), scratch + plain chitosan composite (0%), scratch + 1% copper solution Cu-Ch (1%), and scratch + 2% copper solution Cu-Ch (2%) conditions.
29450536	3	13	theme	scratch	689:695	arg1	%					812:812	2%	811:812	2%	811:812	Methods Human corneal epithelial (HCE) and human limbal epithelial (HLE) cells were monitored under no-scratch (CON), untreated scratch (CS), scratch + plain chitosan composite (0%), scratch + 1% copper solution Cu-Ch (1%), and scratch + 2% copper solution Cu-Ch (2%) conditions.
29450536	3	14	theme	corneal	561:567	arg1	HCE					581:583	Human corneal epithelial (HCE) and human limbal epithelial (HLE) cells	555:624	HCE	581:583	Methods Human corneal epithelial (HCE) and human limbal epithelial (HLE) cells were monitored under no-scratch (CON), untreated scratch (CS), scratch + plain chitosan composite (0%), scratch + 1% copper solution Cu-Ch (1%), and scratch + 2% copper solution Cu-Ch (2%) conditions.
29450536	2	15	theme	injury	539:544	arg1	model					484:488	an in vitro model	472:488	an in vitro model of corneal epithelial and limbal epithelial cell injury	472:544	We determined the efficacy of NO, administered using a novel application of copper-chitosan treatments (Cu-Ch), in facilitating corneal epithelial wound healing using an in vitro model of corneal epithelial and limbal epithelial cell injury.
29450536	11	16	theme	iNOS	1786:1789	arg1	expression					1791:1800	iNOS expression	1786:1800	iNOS expression with treatment	1786:1815	Differences between HCE and HLE responses may be due to intrinsic differences in NO metabolism, as evidenced by differences in NO production, potentially caused by differences in iNOS expression with treatment.
29450536	6	17	dep	showed	1166:1171	arg1	compared					1240:1247	compared	1240:1247	showed a significant decrease in closure with Cu-Ch (1% and 2%) treatment compared to CS	1166:1253	Results Wound closure significantly increased in HCE cells treated with Cu-Ch (1% and 2%) after 72 hours, while HLE cells showed a significant decrease in closure with Cu-Ch (1% and 2%) treatment compared to CS.
29450536	11	18	theme	intrinsic	1663:1671	arg1	differences					1673:1683	intrinsic differences	1663:1683	intrinsic differences in NO metabolism	1663:1700	Differences between HCE and HLE responses may be due to intrinsic differences in NO metabolism, as evidenced by differences in NO production, potentially caused by differences in iNOS expression with treatment.
29450536	3	19	theme	1	740:740	arg1	%					741:741	%	741:741	%	741:741	Methods Human corneal epithelial (HCE) and human limbal epithelial (HLE) cells were monitored under no-scratch (CON), untreated scratch (CS), scratch + plain chitosan composite (0%), scratch + 1% copper solution Cu-Ch (1%), and scratch + 2% copper solution Cu-Ch (2%) conditions.
29450536	2	20	theme	epithelial	523:532	arg1	injury					539:544	corneal epithelial and limbal epithelial cell injury	493:544	injury	539:544	We determined the efficacy of NO, administered using a novel application of copper-chitosan treatments (Cu-Ch), in facilitating corneal epithelial wound healing using an in vitro model of corneal epithelial and limbal epithelial cell injury.
29450536	3	21	theme	composite	714:722	arg1	chitosan					705:712	plain chitosan composite	699:722	plain chitosan composite	699:722	Methods Human corneal epithelial (HCE) and human limbal epithelial (HLE) cells were monitored under no-scratch (CON), untreated scratch (CS), scratch + plain chitosan composite (0%), scratch + 1% copper solution Cu-Ch (1%), and scratch + 2% copper solution Cu-Ch (2%) conditions.
29450536	1	22	theme	Purpose	131:137	arg1	oxide					146:150	Purpose Nitric oxide	131:150	Purpose Nitric oxide (NO)	131:155	Purpose Nitric oxide (NO) has gained attention for its role in facilitating wound healing by promoting cell migration, while being cytoprotective in a variety of cell types.
29450536	1	22	theme	Purpose	131:137	arg1	NO					153:154	NO	153:154	NO	153:154	Purpose Nitric oxide (NO) has gained attention for its role in facilitating wound healing by promoting cell migration, while being cytoprotective in a variety of cell types.
29450536	3	23	theme	scratch	730:736	arg1	conditions					815:824	no-scratch (CON), untreated scratch (CS), scratch + plain chitosan composite (0%), scratch + 1% copper solution Cu-Ch (1%), and scratch + 2% copper solution Cu-Ch (2%) conditions	647:824	no-scratch (CON), untreated scratch (CS), scratch + plain chitosan composite (0%), scratch + 1% copper solution Cu-Ch (1%), and scratch + 2% copper solution Cu-Ch (2%) conditions	647:824	Methods Human corneal epithelial (HCE) and human limbal epithelial (HLE) cells were monitored under no-scratch (CON), untreated scratch (CS), scratch + plain chitosan composite (0%), scratch + 1% copper solution Cu-Ch (1%), and scratch + 2% copper solution Cu-Ch (2%) conditions.
29450536	3	23	theme	scratch	730:736	arg1	%					812:812	2%	811:812	2%	811:812	Methods Human corneal epithelial (HCE) and human limbal epithelial (HLE) cells were monitored under no-scratch (CON), untreated scratch (CS), scratch + plain chitosan composite (0%), scratch + 1% copper solution Cu-Ch (1%), and scratch + 2% copper solution Cu-Ch (2%) conditions.
29450536	7	24	theme	Cu-Ch	1315:1319	arg1	treatments					1321:1330	1% and 2% Cu-Ch treatments	1305:1330	1% and 2% Cu-Ch treatments in HCE cells	1305:1343	Cytotoxic fragments decreased significantly with 1% and 2% Cu-Ch treatments in HCE cells.
29450536	3	25	theme	plain	699:703	arg1	chitosan					705:712	plain chitosan composite	699:722	plain chitosan composite	699:722	Methods Human corneal epithelial (HCE) and human limbal epithelial (HLE) cells were monitored under no-scratch (CON), untreated scratch (CS), scratch + plain chitosan composite (0%), scratch + 1% copper solution Cu-Ch (1%), and scratch + 2% copper solution Cu-Ch (2%) conditions.
29450536	0	26	theme	Delivery	63:70	arg1	System					72:77	a Novel Copper-Chitosan Delivery System	39:77	a Novel Copper-Chitosan Delivery System in Human Corneal and Limbal Epithelial Cell Injury	39:128	Administration of Nitric Oxide Through a Novel Copper-Chitosan Delivery System in Human Corneal and Limbal Epithelial Cell Injury.
29450536	1	27	theme	types	298:302	arg1	variety					282:288	a variety	280:288	a variety of cell types	280:302	Purpose Nitric oxide (NO) has gained attention for its role in facilitating wound healing by promoting cell migration, while being cytoprotective in a variety of cell types.
29450536	1	27	theme	types	298:302	arg1	types					298:302	cell types	293:302	cell types	293:302	Purpose Nitric oxide (NO) has gained attention for its role in facilitating wound healing by promoting cell migration, while being cytoprotective in a variety of cell types.
29450536	10	28	theme	HCE	1533:1535	arg1	healing					1543:1549	HCE wound healing	1533:1549	HCE wound healing	1533:1549	Conclusions Overall, HCE wound healing was accelerated with administration of Cu-Ch treatment.
29450536	2	29	theme	wound	452:456	arg1	healing					458:464	corneal epithelial wound healing	433:464	corneal epithelial wound healing	433:464	We determined the efficacy of NO, administered using a novel application of copper-chitosan treatments (Cu-Ch), in facilitating corneal epithelial wound healing using an in vitro model of corneal epithelial and limbal epithelial cell injury.
29450536	11	30	theme	NO	1688:1689	arg1	metabolism					1691:1700	NO metabolism	1688:1700	NO metabolism	1688:1700	Differences between HCE and HLE responses may be due to intrinsic differences in NO metabolism, as evidenced by differences in NO production, potentially caused by differences in iNOS expression with treatment.
29450536	3	31	theme	copper	743:748	arg1	Cu-Ch					759:763	1% copper solution Cu-Ch	740:763	1% copper solution Cu-Ch (1%)	740:768	Methods Human corneal epithelial (HCE) and human limbal epithelial (HLE) cells were monitored under no-scratch (CON), untreated scratch (CS), scratch + plain chitosan composite (0%), scratch + 1% copper solution Cu-Ch (1%), and scratch + 2% copper solution Cu-Ch (2%) conditions.
29450536	3	31	theme	copper	743:748	arg1	%					767:767	1%	766:767	1%	766:767	Methods Human corneal epithelial (HCE) and human limbal epithelial (HLE) cells were monitored under no-scratch (CON), untreated scratch (CS), scratch + plain chitosan composite (0%), scratch + 1% copper solution Cu-Ch (1%), and scratch + 2% copper solution Cu-Ch (2%) conditions.
29450536	7	32	theme	%	1306:1306	arg1	treatments					1321:1330	1% and 2% Cu-Ch treatments	1305:1330	1% and 2% Cu-Ch treatments in HCE cells	1305:1343	Cytotoxic fragments decreased significantly with 1% and 2% Cu-Ch treatments in HCE cells.
29450536	11	33	from	differences	1771:1781	arg1	expression					1791:1800	iNOS expression	1786:1800	iNOS expression with treatment	1786:1815	Differences between HCE and HLE responses may be due to intrinsic differences in NO metabolism, as evidenced by differences in NO production, potentially caused by differences in iNOS expression with treatment.
29450536	2	34	theme	in	475:476	arg1	model					484:488	an in vitro model	472:488	an in vitro model of corneal epithelial and limbal epithelial cell injury	472:544	We determined the efficacy of NO, administered using a novel application of copper-chitosan treatments (Cu-Ch), in facilitating corneal epithelial wound healing using an in vitro model of corneal epithelial and limbal epithelial cell injury.
29450536	0	35	theme	Limbal	100:105	arg1	Injury					123:128	Human Corneal and Limbal Epithelial Cell Injury	82:128	Injury	123:128	Administration of Nitric Oxide Through a Novel Copper-Chitosan Delivery System in Human Corneal and Limbal Epithelial Cell Injury.
29450536	11	36	with	expression	1791:1800	arg1	treatment					1807:1815	treatment	1807:1815	treatment	1807:1815	Differences between HCE and HLE responses may be due to intrinsic differences in NO metabolism, as evidenced by differences in NO production, potentially caused by differences in iNOS expression with treatment.
29450536	5	37	theme	iNOS	975:978	arg1	expression					980:989	iNOS expression	975:989	iNOS expression in HLE cells	975:1002	iNOS expression in HLE cells also was determined using Western blot.
29450536	10	38	theme	wound	1537:1541	arg1	healing					1543:1549	HCE wound healing	1533:1549	HCE wound healing	1533:1549	Conclusions Overall, HCE wound healing was accelerated with administration of Cu-Ch treatment.
29450536	2	39	theme	copper-chitosan	381:395	arg1	Cu-Ch					409:413	Cu-Ch	409:413	Cu-Ch	409:413	We determined the efficacy of NO, administered using a novel application of copper-chitosan treatments (Cu-Ch), in facilitating corneal epithelial wound healing using an in vitro model of corneal epithelial and limbal epithelial cell injury.
29450536	2	39	theme	copper-chitosan	381:395	arg1	treatments					397:406	copper-chitosan treatments	381:406	copper-chitosan treatments (Cu-Ch)	381:414	We determined the efficacy of NO, administered using a novel application of copper-chitosan treatments (Cu-Ch), in facilitating corneal epithelial wound healing using an in vitro model of corneal epithelial and limbal epithelial cell injury.
29450536	0	40	theme	Nitric	18:23	arg1	Oxide					25:29	Nitric Oxide	18:29	Nitric Oxide	18:29	Administration of Nitric Oxide Through a Novel Copper-Chitosan Delivery System in Human Corneal and Limbal Epithelial Cell Injury.
29450536	2	41	theme	corneal	433:439	arg1	healing					458:464	corneal epithelial wound healing	433:464	corneal epithelial wound healing	433:464	We determined the efficacy of NO, administered using a novel application of copper-chitosan treatments (Cu-Ch), in facilitating corneal epithelial wound healing using an in vitro model of corneal epithelial and limbal epithelial cell injury.
29450536	3	42	theme	epithelial	603:612	arg1	cells					620:624	Human corneal epithelial (HCE) and human limbal epithelial (HLE) cells	555:624	cells	620:624	Methods Human corneal epithelial (HCE) and human limbal epithelial (HLE) cells were monitored under no-scratch (CON), untreated scratch (CS), scratch + plain chitosan composite (0%), scratch + 1% copper solution Cu-Ch (1%), and scratch + 2% copper solution Cu-Ch (2%) conditions.
29450536	3	43	theme	solution	795:802	arg1	conditions					815:824	no-scratch (CON), untreated scratch (CS), scratch + plain chitosan composite (0%), scratch + 1% copper solution Cu-Ch (1%), and scratch + 2% copper solution Cu-Ch (2%) conditions	647:824	no-scratch (CON), untreated scratch (CS), scratch + plain chitosan composite (0%), scratch + 1% copper solution Cu-Ch (1%), and scratch + 2% copper solution Cu-Ch (2%) conditions	647:824	Methods Human corneal epithelial (HCE) and human limbal epithelial (HLE) cells were monitored under no-scratch (CON), untreated scratch (CS), scratch + plain chitosan composite (0%), scratch + 1% copper solution Cu-Ch (1%), and scratch + 2% copper solution Cu-Ch (2%) conditions.
29450536	3	43	theme	solution	795:802	arg1	%					812:812	2%	811:812	2%	811:812	Methods Human corneal epithelial (HCE) and human limbal epithelial (HLE) cells were monitored under no-scratch (CON), untreated scratch (CS), scratch + plain chitosan composite (0%), scratch + 1% copper solution Cu-Ch (1%), and scratch + 2% copper solution Cu-Ch (2%) conditions.
29450536	3	44	theme	human	590:594	arg1	cells					620:624	Human corneal epithelial (HCE) and human limbal epithelial (HLE) cells	555:624	cells	620:624	Methods Human corneal epithelial (HCE) and human limbal epithelial (HLE) cells were monitored under no-scratch (CON), untreated scratch (CS), scratch + plain chitosan composite (0%), scratch + 1% copper solution Cu-Ch (1%), and scratch + 2% copper solution Cu-Ch (2%) conditions.
29450536	0	45	theme	Novel	41:45	arg1	System					72:77	a Novel Copper-Chitosan Delivery System	39:77	a Novel Copper-Chitosan Delivery System in Human Corneal and Limbal Epithelial Cell Injury	39:128	Administration of Nitric Oxide Through a Novel Copper-Chitosan Delivery System in Human Corneal and Limbal Epithelial Cell Injury.
29450536	3	46	theme	%	786:786	arg1	conditions					815:824	no-scratch (CON), untreated scratch (CS), scratch + plain chitosan composite (0%), scratch + 1% copper solution Cu-Ch (1%), and scratch + 2% copper solution Cu-Ch (2%) conditions	647:824	no-scratch (CON), untreated scratch (CS), scratch + plain chitosan composite (0%), scratch + 1% copper solution Cu-Ch (1%), and scratch + 2% copper solution Cu-Ch (2%) conditions	647:824	Methods Human corneal epithelial (HCE) and human limbal epithelial (HLE) cells were monitored under no-scratch (CON), untreated scratch (CS), scratch + plain chitosan composite (0%), scratch + 1% copper solution Cu-Ch (1%), and scratch + 2% copper solution Cu-Ch (2%) conditions.
29450536	3	46	theme	%	786:786	arg1	%					812:812	2%	811:812	2%	811:812	Methods Human corneal epithelial (HCE) and human limbal epithelial (HLE) cells were monitored under no-scratch (CON), untreated scratch (CS), scratch + plain chitosan composite (0%), scratch + 1% copper solution Cu-Ch (1%), and scratch + 2% copper solution Cu-Ch (2%) conditions.
29450536	8	47	with	increase	1403:1410	arg1	treatment					1426:1434	2% Cu-Ch treatment	1417:1434	2% Cu-Ch treatment	1417:1434	Nitrate/nitrite levels in HLE cells showed a significant increase with 2% Cu-Ch treatment compared to CS.
29450536	8	48	theme	HLE	1372:1374	arg1	cells					1376:1380	HLE cells	1372:1380	HLE cells	1372:1380	Nitrate/nitrite levels in HLE cells showed a significant increase with 2% Cu-Ch treatment compared to CS.
29450536	3	49	theme	HLE	615:617	arg1	cells					620:624	Human corneal epithelial (HCE) and human limbal epithelial (HLE) cells	555:624	cells	620:624	Methods Human corneal epithelial (HCE) and human limbal epithelial (HLE) cells were monitored under no-scratch (CON), untreated scratch (CS), scratch + plain chitosan composite (0%), scratch + 1% copper solution Cu-Ch (1%), and scratch + 2% copper solution Cu-Ch (2%) conditions.
29450536	2	50	theme	NO	335:336	arg1	efficacy					323:330	the efficacy	319:330	the efficacy	319:330	We determined the efficacy of NO, administered using a novel application of copper-chitosan treatments (Cu-Ch), in facilitating corneal epithelial wound healing using an in vitro model of corneal epithelial and limbal epithelial cell injury.
29450536	3	51	theme	no-scratch	647:656	arg1	conditions					815:824	no-scratch (CON), untreated scratch (CS), scratch + plain chitosan composite (0%), scratch + 1% copper solution Cu-Ch (1%), and scratch + 2% copper solution Cu-Ch (2%) conditions	647:824	no-scratch (CON), untreated scratch (CS), scratch + plain chitosan composite (0%), scratch + 1% copper solution Cu-Ch (1%), and scratch + 2% copper solution Cu-Ch (2%) conditions	647:824	Methods Human corneal epithelial (HCE) and human limbal epithelial (HLE) cells were monitored under no-scratch (CON), untreated scratch (CS), scratch + plain chitosan composite (0%), scratch + 1% copper solution Cu-Ch (1%), and scratch + 2% copper solution Cu-Ch (2%) conditions.
29450536	3	51	theme	no-scratch	647:656	arg1	%					812:812	2%	811:812	2%	811:812	Methods Human corneal epithelial (HCE) and human limbal epithelial (HLE) cells were monitored under no-scratch (CON), untreated scratch (CS), scratch + plain chitosan composite (0%), scratch + 1% copper solution Cu-Ch (1%), and scratch + 2% copper solution Cu-Ch (2%) conditions.
29450536	8	52	theme	significant	1391:1401	arg1	increase					1403:1410	a significant increase	1389:1410	a significant increase with 2% Cu-Ch treatment	1389:1434	Nitrate/nitrite levels in HLE cells showed a significant increase with 2% Cu-Ch treatment compared to CS.
29450536	4	53	theme	nitrate/nitrite	878:892	arg1	concentrations					894:907	total nitrate/nitrite concentrations	872:907	total nitrate/nitrite concentrations	872:907	Cell migration, cytotoxicity, apoptosis, and total nitrate/nitrite concentrations were measured at 24, 48, and 72 hours after injury and treatment.
29450536	6	54	theme	Cu-Ch	1212:1216	arg1	treatment					1230:1238	Cu-Ch (1% and 2%) treatment	1212:1238	Cu-Ch (1% and 2%) treatment	1212:1238	Results Wound closure significantly increased in HCE cells treated with Cu-Ch (1% and 2%) after 72 hours, while HLE cells showed a significant decrease in closure with Cu-Ch (1% and 2%) treatment compared to CS.
29450536	3	55	theme	Cu-Ch	804:808	arg1	conditions					815:824	no-scratch (CON), untreated scratch (CS), scratch + plain chitosan composite (0%), scratch + 1% copper solution Cu-Ch (1%), and scratch + 2% copper solution Cu-Ch (2%) conditions	647:824	no-scratch (CON), untreated scratch (CS), scratch + plain chitosan composite (0%), scratch + 1% copper solution Cu-Ch (1%), and scratch + 2% copper solution Cu-Ch (2%) conditions	647:824	Methods Human corneal epithelial (HCE) and human limbal epithelial (HLE) cells were monitored under no-scratch (CON), untreated scratch (CS), scratch + plain chitosan composite (0%), scratch + 1% copper solution Cu-Ch (1%), and scratch + 2% copper solution Cu-Ch (2%) conditions.
29450536	3	55	theme	Cu-Ch	804:808	arg1	%					812:812	2%	811:812	2%	811:812	Methods Human corneal epithelial (HCE) and human limbal epithelial (HLE) cells were monitored under no-scratch (CON), untreated scratch (CS), scratch + plain chitosan composite (0%), scratch + 1% copper solution Cu-Ch (1%), and scratch + 2% copper solution Cu-Ch (2%) conditions.
29450536	1	56	from	cytoprotective	262:275	arg1	variety					282:288	a variety	280:288	a variety of cell types	280:302	Purpose Nitric oxide (NO) has gained attention for its role in facilitating wound healing by promoting cell migration, while being cytoprotective in a variety of cell types.
29450536	1	56	from	cytoprotective	262:275	arg1	types					298:302	cell types	293:302	cell types	293:302	Purpose Nitric oxide (NO) has gained attention for its role in facilitating wound healing by promoting cell migration, while being cytoprotective in a variety of cell types.
29450536	8	57	theme	%	1418:1418	arg1	treatment					1426:1434	2% Cu-Ch treatment	1417:1434	2% Cu-Ch treatment	1417:1434	Nitrate/nitrite levels in HLE cells showed a significant increase with 2% Cu-Ch treatment compared to CS.
29450536	6	58	theme	Wound	1052:1056	arg1	closure					1058:1064	Results Wound closure	1044:1064	Results Wound closure	1044:1064	Results Wound closure significantly increased in HCE cells treated with Cu-Ch (1% and 2%) after 72 hours, while HLE cells showed a significant decrease in closure with Cu-Ch (1% and 2%) treatment compared to CS.
29450536	5	59	from	expression	980:989	arg1	cells					998:1002	HLE cells	994:1002	HLE cells	994:1002	iNOS expression in HLE cells also was determined using Western blot.
29450536	11	60	theme	NO	1734:1735	arg1	production					1737:1746	NO production	1734:1746	NO production	1734:1746	Differences between HCE and HLE responses may be due to intrinsic differences in NO metabolism, as evidenced by differences in NO production, potentially caused by differences in iNOS expression with treatment.
29450536	6	61	dep	Cu-Ch	1116:1120	arg1	%					1124:1124	1%	1123:1124	1%	1123:1124	Results Wound closure significantly increased in HCE cells treated with Cu-Ch (1% and 2%) after 72 hours, while HLE cells showed a significant decrease in closure with Cu-Ch (1% and 2%) treatment compared to CS.
29450536	6	61	dep	Cu-Ch	1116:1120	arg1	%					1131:1131	2%	1130:1131	2%	1130:1131	Results Wound closure significantly increased in HCE cells treated with Cu-Ch (1% and 2%) after 72 hours, while HLE cells showed a significant decrease in closure with Cu-Ch (1% and 2%) treatment compared to CS.
29450536	2	62	theme	epithelial	501:510	arg1	model					484:488	an in vitro model	472:488	an in vitro model of corneal epithelial and limbal epithelial cell injury	472:544	We determined the efficacy of NO, administered using a novel application of copper-chitosan treatments (Cu-Ch), in facilitating corneal epithelial wound healing using an in vitro model of corneal epithelial and limbal epithelial cell injury.
29450536	11	63	from	differences	1673:1683	arg1	metabolism					1691:1700	NO metabolism	1688:1700	NO metabolism	1688:1700	Differences between HCE and HLE responses may be due to intrinsic differences in NO metabolism, as evidenced by differences in NO production, potentially caused by differences in iNOS expression with treatment.
29450536	3	64	theme	scratch	675:681	arg1	conditions					815:824	no-scratch (CON), untreated scratch (CS), scratch + plain chitosan composite (0%), scratch + 1% copper solution Cu-Ch (1%), and scratch + 2% copper solution Cu-Ch (2%) conditions	647:824	no-scratch (CON), untreated scratch (CS), scratch + plain chitosan composite (0%), scratch + 1% copper solution Cu-Ch (1%), and scratch + 2% copper solution Cu-Ch (2%) conditions	647:824	Methods Human corneal epithelial (HCE) and human limbal epithelial (HLE) cells were monitored under no-scratch (CON), untreated scratch (CS), scratch + plain chitosan composite (0%), scratch + 1% copper solution Cu-Ch (1%), and scratch + 2% copper solution Cu-Ch (2%) conditions.
29450536	3	64	theme	scratch	675:681	arg1	%					812:812	2%	811:812	2%	811:812	Methods Human corneal epithelial (HCE) and human limbal epithelial (HLE) cells were monitored under no-scratch (CON), untreated scratch (CS), scratch + plain chitosan composite (0%), scratch + 1% copper solution Cu-Ch (1%), and scratch + 2% copper solution Cu-Ch (2%) conditions.
29450536	6	65	from	decrease	1187:1194	arg1	closure					1199:1205	closure	1199:1205	closure with Cu-Ch (1% and 2%) treatment	1199:1238	Results Wound closure significantly increased in HCE cells treated with Cu-Ch (1% and 2%) after 72 hours, while HLE cells showed a significant decrease in closure with Cu-Ch (1% and 2%) treatment compared to CS.
29450536	3	66	theme	Human	555:559	arg1	HCE					581:583	Human corneal epithelial (HCE) and human limbal epithelial (HLE) cells	555:624	HCE	581:583	Methods Human corneal epithelial (HCE) and human limbal epithelial (HLE) cells were monitored under no-scratch (CON), untreated scratch (CS), scratch + plain chitosan composite (0%), scratch + 1% copper solution Cu-Ch (1%), and scratch + 2% copper solution Cu-Ch (2%) conditions.
29450536	7	67	theme	HCE	1335:1337	arg1	cells					1339:1343	HCE cells	1335:1343	HCE cells	1335:1343	Cytotoxic fragments decreased significantly with 1% and 2% Cu-Ch treatments in HCE cells.
29450536	0	68	from	System	72:77	arg1	Injury					123:128	Human Corneal and Limbal Epithelial Cell Injury	82:128	Injury	123:128	Administration of Nitric Oxide Through a Novel Copper-Chitosan Delivery System in Human Corneal and Limbal Epithelial Cell Injury.
29450536	0	68	from	System	72:77	arg1	Corneal					88:94	Human Corneal and Limbal Epithelial Cell Injury	82:128	Corneal	88:94	Administration of Nitric Oxide Through a Novel Copper-Chitosan Delivery System in Human Corneal and Limbal Epithelial Cell Injury.
29450536	2	69	theme	cell	534:537	arg1	injury					539:544	corneal epithelial and limbal epithelial cell injury	493:544	injury	539:544	We determined the efficacy of NO, administered using a novel application of copper-chitosan treatments (Cu-Ch), in facilitating corneal epithelial wound healing using an in vitro model of corneal epithelial and limbal epithelial cell injury.
29450536	3	70	theme	%	741:741	arg1	Cu-Ch					759:763	1% copper solution Cu-Ch	740:763	1% copper solution Cu-Ch (1%)	740:768	Methods Human corneal epithelial (HCE) and human limbal epithelial (HLE) cells were monitored under no-scratch (CON), untreated scratch (CS), scratch + plain chitosan composite (0%), scratch + 1% copper solution Cu-Ch (1%), and scratch + 2% copper solution Cu-Ch (2%) conditions.
29450536	3	70	theme	%	741:741	arg1	%					767:767	1%	766:767	1%	766:767	Methods Human corneal epithelial (HCE) and human limbal epithelial (HLE) cells were monitored under no-scratch (CON), untreated scratch (CS), scratch + plain chitosan composite (0%), scratch + 1% copper solution Cu-Ch (1%), and scratch + 2% copper solution Cu-Ch (2%) conditions.
29450536	7	71	theme	%	1313:1313	arg1	treatments					1321:1330	1% and 2% Cu-Ch treatments	1305:1330	1% and 2% Cu-Ch treatments in HCE cells	1305:1343	Cytotoxic fragments decreased significantly with 1% and 2% Cu-Ch treatments in HCE cells.
29450536	2	72	theme	limbal	516:521	arg1	injury					539:544	corneal epithelial and limbal epithelial cell injury	493:544	injury	539:544	We determined the efficacy of NO, administered using a novel application of copper-chitosan treatments (Cu-Ch), in facilitating corneal epithelial wound healing using an in vitro model of corneal epithelial and limbal epithelial cell injury.
29450536	6	73	theme	HLE	1156:1158	arg1	cells					1160:1164	HLE cells	1156:1164	HLE cells	1156:1164	Results Wound closure significantly increased in HCE cells treated with Cu-Ch (1% and 2%) after 72 hours, while HLE cells showed a significant decrease in closure with Cu-Ch (1% and 2%) treatment compared to CS.
29450536	3	74	theme	epithelial	569:578	arg1	HCE					581:583	Human corneal epithelial (HCE) and human limbal epithelial (HLE) cells	555:624	HCE	581:583	Methods Human corneal epithelial (HCE) and human limbal epithelial (HLE) cells were monitored under no-scratch (CON), untreated scratch (CS), scratch + plain chitosan composite (0%), scratch + 1% copper solution Cu-Ch (1%), and scratch + 2% copper solution Cu-Ch (2%) conditions.
29450536	1	75	theme	Nitric	139:144	arg1	oxide					146:150	Purpose Nitric oxide	131:150	Purpose Nitric oxide (NO)	131:155	Purpose Nitric oxide (NO) has gained attention for its role in facilitating wound healing by promoting cell migration, while being cytoprotective in a variety of cell types.
29450536	1	75	theme	Nitric	139:144	arg1	NO					153:154	NO	153:154	NO	153:154	Purpose Nitric oxide (NO) has gained attention for its role in facilitating wound healing by promoting cell migration, while being cytoprotective in a variety of cell types.
29450536	0	76	theme	Human	82:86	arg1	Corneal					88:94	Human Corneal and Limbal Epithelial Cell Injury	82:128	Corneal	88:94	Administration of Nitric Oxide Through a Novel Copper-Chitosan Delivery System in Human Corneal and Limbal Epithelial Cell Injury.
29450536	7	77	from	treatments	1321:1330	arg1	cells					1339:1343	HCE cells	1335:1343	HCE cells	1335:1343	Cytotoxic fragments decreased significantly with 1% and 2% Cu-Ch treatments in HCE cells.
29450536	2	78	theme	epithelial	441:450	arg1	healing					458:464	corneal epithelial wound healing	433:464	corneal epithelial wound healing	433:464	We determined the efficacy of NO, administered using a novel application of copper-chitosan treatments (Cu-Ch), in facilitating corneal epithelial wound healing using an in vitro model of corneal epithelial and limbal epithelial cell injury.
29450536	3	79	theme	solution	750:757	arg1	Cu-Ch					759:763	1% copper solution Cu-Ch	740:763	1% copper solution Cu-Ch (1%)	740:768	Methods Human corneal epithelial (HCE) and human limbal epithelial (HLE) cells were monitored under no-scratch (CON), untreated scratch (CS), scratch + plain chitosan composite (0%), scratch + 1% copper solution Cu-Ch (1%), and scratch + 2% copper solution Cu-Ch (2%) conditions.
29450536	3	79	theme	solution	750:757	arg1	%					767:767	1%	766:767	1%	766:767	Methods Human corneal epithelial (HCE) and human limbal epithelial (HLE) cells were monitored under no-scratch (CON), untreated scratch (CS), scratch + plain chitosan composite (0%), scratch + 1% copper solution Cu-Ch (1%), and scratch + 2% copper solution Cu-Ch (2%) conditions.
29450536	0	80	theme	Epithelial	107:116	arg1	Injury					123:128	Human Corneal and Limbal Epithelial Cell Injury	82:128	Injury	123:128	Administration of Nitric Oxide Through a Novel Copper-Chitosan Delivery System in Human Corneal and Limbal Epithelial Cell Injury.
29450536	6	81	with	closure	1199:1205	arg1	treatment					1230:1238	Cu-Ch (1% and 2%) treatment	1212:1238	Cu-Ch (1% and 2%) treatment	1212:1238	Results Wound closure significantly increased in HCE cells treated with Cu-Ch (1% and 2%) after 72 hours, while HLE cells showed a significant decrease in closure with Cu-Ch (1% and 2%) treatment compared to CS.
29450536	9	82	theme	iNOS	1506:1509	arg1	upregulation					1490:1501	an upregulation	1487:1501	an upregulation of iNOS	1487:1509	This increase is complemented with an upregulation of iNOS.
29450536	1	83	theme	wound	207:211	arg1	healing					213:219	wound healing	207:219	wound healing	207:219	Purpose Nitric oxide (NO) has gained attention for its role in facilitating wound healing by promoting cell migration, while being cytoprotective in a variety of cell types.
29450536	11	84	theme	HLE	1635:1637	arg1	responses					1639:1647	HLE responses	1635:1647	HLE responses	1635:1647	Differences between HCE and HLE responses may be due to intrinsic differences in NO metabolism, as evidenced by differences in NO production, potentially caused by differences in iNOS expression with treatment.
29450536	10	85	theme	Cu-Ch	1590:1594	arg1	treatment					1596:1604	Cu-Ch treatment	1590:1604	Cu-Ch treatment	1590:1604	Conclusions Overall, HCE wound healing was accelerated with administration of Cu-Ch treatment.
29450536	0	86	theme	Oxide	25:29	arg1	Administration					0:13	Administration	0:13	Administration of Nitric Oxide Through a Novel Copper-Chitosan Delivery System in Human Corneal and Limbal Epithelial Cell Injury.	0:129	Administration of Nitric Oxide Through a Novel Copper-Chitosan Delivery System in Human Corneal and Limbal Epithelial Cell Injury.
29450536	2	87	dep	in	475:476	arg1	vitro					478:482	vitro	478:482	vitro	478:482	We determined the efficacy of NO, administered using a novel application of copper-chitosan treatments (Cu-Ch), in facilitating corneal epithelial wound healing using an in vitro model of corneal epithelial and limbal epithelial cell injury.
29450536	2	88	theme	treatments	397:406	arg1	application					366:376	a novel application	358:376	a novel application of copper-chitosan treatments (Cu-Ch)	358:414	We determined the efficacy of NO, administered using a novel application of copper-chitosan treatments (Cu-Ch), in facilitating corneal epithelial wound healing using an in vitro model of corneal epithelial and limbal epithelial cell injury.
29450536	3	89	theme	limbal	596:601	arg1	cells					620:624	Human corneal epithelial (HCE) and human limbal epithelial (HLE) cells	555:624	cells	620:624	Methods Human corneal epithelial (HCE) and human limbal epithelial (HLE) cells were monitored under no-scratch (CON), untreated scratch (CS), scratch + plain chitosan composite (0%), scratch + 1% copper solution Cu-Ch (1%), and scratch + 2% copper solution Cu-Ch (2%) conditions.
29450536	6	90	theme	significant	1175:1185	arg1	decrease					1187:1194	a significant decrease	1173:1194	a significant decrease in closure with Cu-Ch (1% and 2%) treatment	1173:1238	Results Wound closure significantly increased in HCE cells treated with Cu-Ch (1% and 2%) after 72 hours, while HLE cells showed a significant decrease in closure with Cu-Ch (1% and 2%) treatment compared to CS.
29450536	5	91	theme	HLE	994:996	arg1	cells					998:1002	HLE cells	994:1002	HLE cells	994:1002	iNOS expression in HLE cells also was determined using Western blot.
29450536	3	92	theme	scratch	775:781	arg1	conditions					815:824	no-scratch (CON), untreated scratch (CS), scratch + plain chitosan composite (0%), scratch + 1% copper solution Cu-Ch (1%), and scratch + 2% copper solution Cu-Ch (2%) conditions	647:824	no-scratch (CON), untreated scratch (CS), scratch + plain chitosan composite (0%), scratch + 1% copper solution Cu-Ch (1%), and scratch + 2% copper solution Cu-Ch (2%) conditions	647:824	Methods Human corneal epithelial (HCE) and human limbal epithelial (HLE) cells were monitored under no-scratch (CON), untreated scratch (CS), scratch + plain chitosan composite (0%), scratch + 1% copper solution Cu-Ch (1%), and scratch + 2% copper solution Cu-Ch (2%) conditions.
29450536	3	92	theme	scratch	775:781	arg1	%					812:812	2%	811:812	2%	811:812	Methods Human corneal epithelial (HCE) and human limbal epithelial (HLE) cells were monitored under no-scratch (CON), untreated scratch (CS), scratch + plain chitosan composite (0%), scratch + 1% copper solution Cu-Ch (1%), and scratch + 2% copper solution Cu-Ch (2%) conditions.
29450536	2	93	theme	novel	360:364	arg1	application					366:376	a novel application	358:376	a novel application of copper-chitosan treatments (Cu-Ch)	358:414	We determined the efficacy of NO, administered using a novel application of copper-chitosan treatments (Cu-Ch), in facilitating corneal epithelial wound healing using an in vitro model of corneal epithelial and limbal epithelial cell injury.
29450536	0	94	theme	Copper-Chitosan	47:61	arg1	System					72:77	a Novel Copper-Chitosan Delivery System	39:77	a Novel Copper-Chitosan Delivery System in Human Corneal and Limbal Epithelial Cell Injury	39:128	Administration of Nitric Oxide Through a Novel Copper-Chitosan Delivery System in Human Corneal and Limbal Epithelial Cell Injury.
29450536	7	95	theme	Cytotoxic	1256:1264	arg1	fragments					1266:1274	Cytotoxic fragments	1256:1274	Cytotoxic fragments	1256:1274	Cytotoxic fragments decreased significantly with 1% and 2% Cu-Ch treatments in HCE cells.
29450536	6	96	dep	Cu-Ch	1212:1216	arg1	%					1220:1220	1%	1219:1220	1%	1219:1220	Results Wound closure significantly increased in HCE cells treated with Cu-Ch (1% and 2%) after 72 hours, while HLE cells showed a significant decrease in closure with Cu-Ch (1% and 2%) treatment compared to CS.
29450536	6	96	dep	Cu-Ch	1212:1216	arg1	%					1227:1227	2%	1226:1227	2%	1226:1227	Results Wound closure significantly increased in HCE cells treated with Cu-Ch (1% and 2%) after 72 hours, while HLE cells showed a significant decrease in closure with Cu-Ch (1% and 2%) treatment compared to CS.
29450536	4	97	theme	Cell	827:830	arg1	migration					832:840	Cell migration	827:840	Cell migration	827:840	Cell migration, cytotoxicity, apoptosis, and total nitrate/nitrite concentrations were measured at 24, 48, and 72 hours after injury and treatment.
29450536	1	98	theme	cell	293:296	arg1	types					298:302	cell types	293:302	cell types	293:302	Purpose Nitric oxide (NO) has gained attention for its role in facilitating wound healing by promoting cell migration, while being cytoprotective in a variety of cell types.
29450536	3	99	theme	copper	788:793	arg1	conditions					815:824	no-scratch (CON), untreated scratch (CS), scratch + plain chitosan composite (0%), scratch + 1% copper solution Cu-Ch (1%), and scratch + 2% copper solution Cu-Ch (2%) conditions	647:824	no-scratch (CON), untreated scratch (CS), scratch + plain chitosan composite (0%), scratch + 1% copper solution Cu-Ch (1%), and scratch + 2% copper solution Cu-Ch (2%) conditions	647:824	Methods Human corneal epithelial (HCE) and human limbal epithelial (HLE) cells were monitored under no-scratch (CON), untreated scratch (CS), scratch + plain chitosan composite (0%), scratch + 1% copper solution Cu-Ch (1%), and scratch + 2% copper solution Cu-Ch (2%) conditions.
29450536	3	99	theme	copper	788:793	arg1	%					812:812	2%	811:812	2%	811:812	Methods Human corneal epithelial (HCE) and human limbal epithelial (HLE) cells were monitored under no-scratch (CON), untreated scratch (CS), scratch + plain chitosan composite (0%), scratch + 1% copper solution Cu-Ch (1%), and scratch + 2% copper solution Cu-Ch (2%) conditions.
29450536	10	100	theme	treatment	1596:1604	arg1	administration					1572:1585	administration	1572:1585	administration of Cu-Ch treatment	1572:1604	Conclusions Overall, HCE wound healing was accelerated with administration of Cu-Ch treatment.
29450536	5	101	theme	Western	1030:1036	arg1	blot					1038:1041	Western blot	1030:1041	Western blot	1030:1041	iNOS expression in HLE cells also was determined using Western blot.
30893028	7	0	dep	C15 	800:803	arg1	C16 					817:820	C16 	817:820	C16 	817:820	The major fatty acids were C15 : 0 anteiso, C16 : 0, and C16 : 1 ω7c.
30893028	7	0	dep	C15 	800:803	arg1	 1 ω7c					835:840	 1 ω7c	835:840	 1 ω7c	835:840	The major fatty acids were C15 : 0 anteiso, C16 : 0, and C16 : 1 ω7c.
30893028	7	0	dep	C15 	800:803	arg1	 0					822:823	 0	822:823	 0	822:823	The major fatty acids were C15 : 0 anteiso, C16 : 0, and C16 : 1 ω7c.
30893028	7	0	dep	C15 	800:803	arg1	C16 					830:833	C16 	830:833	C16 	830:833	The major fatty acids were C15 : 0 anteiso, C16 : 0, and C16 : 1 ω7c.
30893028	3	1	dep	comparisons	298:308	arg1	the					251:253	the	251:253	the	251:253	On the basis of 16S rRNA gene sequence similarity comparisons, strain TB-66Tgrouped together with Filibacter limicola showing a 16S rRNA gene sequence similarity of 98.5 % to the type strain.
30893028	3	1	dep	comparisons	298:308	arg1	basis					255:259	basis	255:259	basis	255:259	On the basis of 16S rRNA gene sequence similarity comparisons, strain TB-66Tgrouped together with Filibacter limicola showing a 16S rRNA gene sequence similarity of 98.5 % to the type strain.
30893028	8	2	theme	mol	894:896	arg1	G+C content					847:857	The G+C content	843:857	The G+C content of the genomic DNA	843:876	The G+C content of the genomic DNA was 37.6 (±1.8) mol%.
30893028	8	2	theme	mol	894:896	arg1	%					897:897	37.6 (±1.8) mol%	882:897	37.6 (±1.8) mol%	882:897	The G+C content of the genomic DNA was 37.6 (±1.8) mol%.
30893028	3	3	theme	type	427:430	arg1	strain					432:437	the type strain	423:437	the type strain	423:437	On the basis of 16S rRNA gene sequence similarity comparisons, strain TB-66Tgrouped together with Filibacter limicola showing a 16S rRNA gene sequence similarity of 98.5 % to the type strain.
30893028	9	4	dep	13886T	1114:1119	arg1	Filibacter					1090:1099	the genus, Filibacter limicola DSM 13886T	1079:1119	Filibacter	1090:1099	On the basis of the genotypic and phenotypic properties it is clear that strain TB-66T represents a member of the genus Filibacter, but is distinct from the only other species in the genus, Filibacter limicola DSM 13886T.
30893028	5	5	theme	polar	525:529	arg1	profile					537:543	The polar lipid profile	521:543	The polar lipid profile	521:543	The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylserine and three unidentified phospholipids.
30893028	9	6	theme	strain	973:978	arg1	TB-66T					980:985	strain TB-66T	973:985	strain TB-66T	973:985	On the basis of the genotypic and phenotypic properties it is clear that strain TB-66T represents a member of the genus Filibacter, but is distinct from the only other species in the genus, Filibacter limicola DSM 13886T.
30893028	4	7	theme	menaquinone	503:513	arg1	MK-7					515:518	menaquinone MK-7	503:518	menaquinone MK-7	503:518	The quinone system of strain TB-66T consisted predominantly of menaquinone MK-7.
30893028	3	8	theme	sequence	278:285	arg1	comparisons					298:308	16S rRNA gene sequence similarity comparisons	264:308	16S rRNA gene sequence similarity comparisons	264:308	On the basis of 16S rRNA gene sequence similarity comparisons, strain TB-66Tgrouped together with Filibacter limicola showing a 16S rRNA gene sequence similarity of 98.5 % to the type strain.
30893028	12	9	theme	type	1197:1200	arg1	TB-66T					1212:1217	TB-66T	1212:1217	TB-66T (= CIP 111629T= LMG 30660T= CCM 8866T)	1212:1256	The type strain is TB-66T (= CIP 111629T= LMG 30660T= CCM 8866T).
30893028	12	9	theme	type	1197:1200	arg1	strain					1202:1207	The type strain	1193:1207	The type strain	1193:1207	The type strain is TB-66T (= CIP 111629T= LMG 30660T= CCM 8866T).
30893028	5	10	theme	lipid	531:535	arg1	profile					537:543	The polar lipid profile	521:543	The polar lipid profile	521:543	The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylserine and three unidentified phospholipids.
30893028	10	11	theme	novel	1135:1139	arg1	species					1141:1147	a novel species	1133:1147	a novel species with the name Filibacter tadaridae sp	1133:1185	We propose a novel species with the name Filibacter tadaridae sp.
30893028	3	12	theme	similarity	287:296	arg1	comparisons					298:308	16S rRNA gene sequence similarity comparisons	264:308	16S rRNA gene sequence similarity comparisons	264:308	On the basis of 16S rRNA gene sequence similarity comparisons, strain TB-66Tgrouped together with Filibacter limicola showing a 16S rRNA gene sequence similarity of 98.5 % to the type strain.
30893028	2	13	theme	Gram-stain-positive	132:150	arg1	bacterium					161:169	A Gram-stain-positive, aerobic bacterium	130:169	A Gram-stain-positive, aerobic bacterium	130:169	A Gram-stain-positive, aerobic bacterium, TB-66T, was isolated from a pile of bat guano in a cave of New Mexico, USA.
30893028	2	13	theme	Gram-stain-positive	132:150	arg1	TB-66T					172:177	TB-66T	172:177	TB-66T	172:177	A Gram-stain-positive, aerobic bacterium, TB-66T, was isolated from a pile of bat guano in a cave of New Mexico, USA.
30893028	9	14	theme	genus	1083:1087	arg1	13886T					1114:1119	the genus, Filibacter limicola DSM 13886T	1079:1119	13886T	1114:1119	On the basis of the genotypic and phenotypic properties it is clear that strain TB-66T represents a member of the genus Filibacter, but is distinct from the only other species in the genus, Filibacter limicola DSM 13886T.
30893028	1	15	dep	Tadarida	107:114	arg1	brasiliensis					116:127	Tadarida brasiliensis	107:127	Tadarida brasiliensis	107:127	nov., isolated from within a guano pile from a colony of Mexican free-tailed bats Tadarida brasiliensis.
30893028	1	15	dep	Tadarida	107:114	arg1	nov.					25:28	nov.	25:28	nov.	25:28	nov., isolated from within a guano pile from a colony of Mexican free-tailed bats Tadarida brasiliensis.
30893028	6	16	theme	peptidoglycan	724:736	arg1	type					738:741	The peptidoglycan type	720:741	The peptidoglycan type	720:741	The peptidoglycan type was A4α l-Lys-d-Glu (A11.33).
30893028	6	16	theme	peptidoglycan	724:736	arg1	l-Lys-d-Glu					751:761	A4α l-Lys-d-Glu	747:761	A4α l-Lys-d-Glu (A11.33)	747:770	The peptidoglycan type was A4α l-Lys-d-Glu (A11.33).
30893028	1	17	attach	isolated	31:38	arg1	colony					72:77	a colony	70:77	a colony of Mexican free-tailed bats	70:105	nov., isolated from within a guano pile from a colony of Mexican free-tailed bats Tadarida brasiliensis.
30893028	1	17	attach	isolated	31:38	arg2	nov.					25:28	nov.	25:28	nov.	25:28	nov., isolated from within a guano pile from a colony of Mexican free-tailed bats Tadarida brasiliensis.
30893028	3	18	theme	rRNA	268:271	arg1	comparisons					298:308	16S rRNA gene sequence similarity comparisons	264:308	16S rRNA gene sequence similarity comparisons	264:308	On the basis of 16S rRNA gene sequence similarity comparisons, strain TB-66Tgrouped together with Filibacter limicola showing a 16S rRNA gene sequence similarity of 98.5 % to the type strain.
30893028	9	19	dep	properties	945:954	arg1	the					903:905	the	903:905	the	903:905	On the basis of the genotypic and phenotypic properties it is clear that strain TB-66T represents a member of the genus Filibacter, but is distinct from the only other species in the genus, Filibacter limicola DSM 13886T.
30893028	9	19	dep	properties	945:954	arg1	basis					907:911	basis	907:911	basis	907:911	On the basis of the genotypic and phenotypic properties it is clear that strain TB-66T represents a member of the genus Filibacter, but is distinct from the only other species in the genus, Filibacter limicola DSM 13886T.
30893028	1	20	theme	guano	54:58	arg1	pile					60:63	a guano pile	52:63	a guano pile	52:63	nov., isolated from within a guano pile from a colony of Mexican free-tailed bats Tadarida brasiliensis.
30893028	10	21	theme	tadaridae	1174:1182	arg1	sp					1184:1185	the name Filibacter tadaridae sp	1154:1185	the name Filibacter tadaridae sp	1154:1185	We propose a novel species with the name Filibacter tadaridae sp.
30893028	3	22	theme	16S	376:378	arg1	similarity					399:408	a 16S rRNA gene sequence similarity	374:408	a 16S rRNA gene sequence similarity of 98.5 % to the type strain	374:437	On the basis of 16S rRNA gene sequence similarity comparisons, strain TB-66Tgrouped together with Filibacter limicola showing a 16S rRNA gene sequence similarity of 98.5 % to the type strain.
30893028	3	23	theme	gene	273:276	arg1	comparisons					298:308	16S rRNA gene sequence similarity comparisons	264:308	16S rRNA gene sequence similarity comparisons	264:308	On the basis of 16S rRNA gene sequence similarity comparisons, strain TB-66Tgrouped together with Filibacter limicola showing a 16S rRNA gene sequence similarity of 98.5 % to the type strain.
30893028	10	24	theme	Filibacter	1163:1172	arg1	sp					1184:1185	the name Filibacter tadaridae sp	1154:1185	the name Filibacter tadaridae sp	1154:1185	We propose a novel species with the name Filibacter tadaridae sp.
30893028	2	25	attach	isolated	184:191	arg1	pile					200:203	a pile	198:203	a pile of bat guano in a cave of New Mexico, USA	198:245	A Gram-stain-positive, aerobic bacterium, TB-66T, was isolated from a pile of bat guano in a cave of New Mexico, USA.
30893028	2	25	attach	isolated	184:191	arg2	TB-66T					172:177	TB-66T	172:177	TB-66T	172:177	A Gram-stain-positive, aerobic bacterium, TB-66T, was isolated from a pile of bat guano in a cave of New Mexico, USA.
30893028	2	25	attach	isolated	184:191	arg2	bacterium					161:169	A Gram-stain-positive, aerobic bacterium	130:169	A Gram-stain-positive, aerobic bacterium	130:169	A Gram-stain-positive, aerobic bacterium, TB-66T, was isolated from a pile of bat guano in a cave of New Mexico, USA.
30893028	0	26	theme	tadaridae	11:19	arg1	sp					21:22	Filibacter tadaridae sp	0:22	Filibacter tadaridae sp.	0:23	Filibacter tadaridae sp.
30893028	9	27	theme	limicola	1101:1108	arg1	13886T					1114:1119	the genus, Filibacter limicola DSM 13886T	1079:1119	13886T	1114:1119	On the basis of the genotypic and phenotypic properties it is clear that strain TB-66T represents a member of the genus Filibacter, but is distinct from the only other species in the genus, Filibacter limicola DSM 13886T.
30893028	12	28	theme	30660T=	1239:1245	arg1	8866T					1251:1255	= CIP 111629T= LMG 30660T= CCM 8866T	1220:1255	= CIP 111629T= LMG 30660T= CCM 8866T	1220:1255	The type strain is TB-66T (= CIP 111629T= LMG 30660T= CCM 8866T).
30893028	12	28	theme	30660T=	1239:1245	arg1	TB-66T					1212:1217	TB-66T	1212:1217	TB-66T (= CIP 111629T= LMG 30660T= CCM 8866T)	1212:1256	The type strain is TB-66T (= CIP 111629T= LMG 30660T= CCM 8866T).
30893028	2	29	from	pile	200:203	arg1	cave					223:226	a cave	221:226	a cave of New Mexico, USA	221:245	A Gram-stain-positive, aerobic bacterium, TB-66T, was isolated from a pile of bat guano in a cave of New Mexico, USA.
30893028	0	30	theme	Filibacter	0:9	arg1	sp					21:22	Filibacter tadaridae sp	0:22	Filibacter tadaridae sp.	0:23	Filibacter tadaridae sp.
30893028	9	31	theme	DSM	1110:1112	arg1	13886T					1114:1119	the genus, Filibacter limicola DSM 13886T	1079:1119	13886T	1114:1119	On the basis of the genotypic and phenotypic properties it is clear that strain TB-66T represents a member of the genus Filibacter, but is distinct from the only other species in the genus, Filibacter limicola DSM 13886T.
30893028	12	32	theme	LMG	1235:1237	arg1	8866T					1251:1255	= CIP 111629T= LMG 30660T= CCM 8866T	1220:1255	= CIP 111629T= LMG 30660T= CCM 8866T	1220:1255	The type strain is TB-66T (= CIP 111629T= LMG 30660T= CCM 8866T).
30893028	12	32	theme	LMG	1235:1237	arg1	TB-66T					1212:1217	TB-66T	1212:1217	TB-66T (= CIP 111629T= LMG 30660T= CCM 8866T)	1212:1256	The type strain is TB-66T (= CIP 111629T= LMG 30660T= CCM 8866T).
30893028	4	33	theme	TB-66T	469:474	arg1	system					452:457	The quinone system	440:457	The quinone system of strain TB-66T	440:474	The quinone system of strain TB-66T consisted predominantly of menaquinone MK-7.
30893028	2	34	theme	USA	243:245	arg1	cave					223:226	a cave	221:226	a cave of New Mexico, USA	221:245	A Gram-stain-positive, aerobic bacterium, TB-66T, was isolated from a pile of bat guano in a cave of New Mexico, USA.
30893028	9	35	theme	genus	1014:1018	arg1	Filibacter					1020:1029	the genus Filibacter	1010:1029	the genus Filibacter	1010:1029	On the basis of the genotypic and phenotypic properties it is clear that strain TB-66T represents a member of the genus Filibacter, but is distinct from the only other species in the genus, Filibacter limicola DSM 13886T.
30893028	2	36	dep	Gram-stain-positive	132:150	arg1	aerobic					153:159	aerobic	153:159	aerobic	153:159	A Gram-stain-positive, aerobic bacterium, TB-66T, was isolated from a pile of bat guano in a cave of New Mexico, USA.
30893028	12	37	theme	CCM	1247:1249	arg1	8866T					1251:1255	= CIP 111629T= LMG 30660T= CCM 8866T	1220:1255	= CIP 111629T= LMG 30660T= CCM 8866T	1220:1255	The type strain is TB-66T (= CIP 111629T= LMG 30660T= CCM 8866T).
30893028	12	37	theme	CCM	1247:1249	arg1	TB-66T					1212:1217	TB-66T	1212:1217	TB-66T (= CIP 111629T= LMG 30660T= CCM 8866T)	1212:1256	The type strain is TB-66T (= CIP 111629T= LMG 30660T= CCM 8866T).
30893028	8	38	theme	genomic	866:872	arg1	DNA					874:876	the genomic DNA	862:876	the genomic DNA	862:876	The G+C content of the genomic DNA was 37.6 (±1.8) mol%.
30893028	12	39	theme	=	1220:1220	arg1	8866T					1251:1255	= CIP 111629T= LMG 30660T= CCM 8866T	1220:1255	= CIP 111629T= LMG 30660T= CCM 8866T	1220:1255	The type strain is TB-66T (= CIP 111629T= LMG 30660T= CCM 8866T).
30893028	12	39	theme	=	1220:1220	arg1	TB-66T					1212:1217	TB-66T	1212:1217	TB-66T (= CIP 111629T= LMG 30660T= CCM 8866T)	1212:1256	The type strain is TB-66T (= CIP 111629T= LMG 30660T= CCM 8866T).
30893028	3	40	theme	gene	385:388	arg1	similarity					399:408	a 16S rRNA gene sequence similarity	374:408	a 16S rRNA gene sequence similarity of 98.5 % to the type strain	374:437	On the basis of 16S rRNA gene sequence similarity comparisons, strain TB-66Tgrouped together with Filibacter limicola showing a 16S rRNA gene sequence similarity of 98.5 % to the type strain.
30893028	5	41	contain	contained	545:553	arg2	phosphatidylserine					663:680	phosphatidylserine	663:680	phosphatidylserine	663:680	The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylserine and three unidentified phospholipids.
30893028	5	41	contain	contained	545:553	arg2	phosphatidylethanolamine					601:624	phosphatidylethanolamine	601:624	phosphatidylethanolamine	601:624	The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylserine and three unidentified phospholipids.
30893028	5	41	contain	contained	545:553	arg2	phosphatidylmonomethylethanolamine					627:660	phosphatidylmonomethylethanolamine	627:660	phosphatidylmonomethylethanolamine	627:660	The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylserine and three unidentified phospholipids.
30893028	5	41	contain	contained	545:553	arg2	phospholipids					705:717	three unidentified phospholipids	686:717	three unidentified phospholipids	686:717	The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylserine and three unidentified phospholipids.
30893028	5	41	contain	contained	545:553	arg1	profile					537:543	The polar lipid profile	521:543	The polar lipid profile	521:543	The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylserine and three unidentified phospholipids.
30893028	5	41	contain	contained	545:553	arg2	diphosphatidylglycerol					555:576	diphosphatidylglycerol	555:576	diphosphatidylglycerol	555:576	The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylserine and three unidentified phospholipids.
30893028	5	41	contain	contained	545:553	arg2	phosphatidylglycerol					579:598	phosphatidylglycerol	579:598	phosphatidylglycerol	579:598	The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylserine and three unidentified phospholipids.
30893028	7	42	theme	fatty	783:787	arg1	C15 					800:803	C15 	800:803	C15 	800:803	The major fatty acids were C15 : 0 anteiso, C16 : 0, and C16 : 1 ω7c.
30893028	7	42	theme	fatty	783:787	arg1	acids					789:793	The major fatty acids	773:793	The major fatty acids	773:793	The major fatty acids were C15 : 0 anteiso, C16 : 0, and C16 : 1 ω7c.
30893028	3	43	theme	sequence	390:397	arg1	similarity					399:408	a 16S rRNA gene sequence similarity	374:408	a 16S rRNA gene sequence similarity of 98.5 % to the type strain	374:437	On the basis of 16S rRNA gene sequence similarity comparisons, strain TB-66Tgrouped together with Filibacter limicola showing a 16S rRNA gene sequence similarity of 98.5 % to the type strain.
30893028	9	44	theme	genotypic	920:928	arg1	properties					945:954	the genotypic and phenotypic properties	916:954	the genotypic and phenotypic properties	916:954	On the basis of the genotypic and phenotypic properties it is clear that strain TB-66T represents a member of the genus Filibacter, but is distinct from the only other species in the genus, Filibacter limicola DSM 13886T.
30893028	4	45	theme	quinone	444:450	arg1	system					452:457	The quinone system	440:457	The quinone system of strain TB-66T	440:474	The quinone system of strain TB-66T consisted predominantly of menaquinone MK-7.
30893028	10	46	with	species	1141:1147	arg1	sp					1184:1185	the name Filibacter tadaridae sp	1154:1185	the name Filibacter tadaridae sp	1154:1185	We propose a novel species with the name Filibacter tadaridae sp.
30893028	12	47	theme	111629T=	1226:1233	arg1	8866T					1251:1255	= CIP 111629T= LMG 30660T= CCM 8866T	1220:1255	= CIP 111629T= LMG 30660T= CCM 8866T	1220:1255	The type strain is TB-66T (= CIP 111629T= LMG 30660T= CCM 8866T).
30893028	12	47	theme	111629T=	1226:1233	arg1	TB-66T					1212:1217	TB-66T	1212:1217	TB-66T (= CIP 111629T= LMG 30660T= CCM 8866T)	1212:1256	The type strain is TB-66T (= CIP 111629T= LMG 30660T= CCM 8866T).
30893028	8	48	theme	DNA	874:876	arg1	G+C content					847:857	The G+C content	843:857	The G+C content of the genomic DNA	843:876	The G+C content of the genomic DNA was 37.6 (±1.8) mol%.
30893028	8	48	theme	DNA	874:876	arg1	%					897:897	37.6 (±1.8) mol%	882:897	37.6 (±1.8) mol%	882:897	The G+C content of the genomic DNA was 37.6 (±1.8) mol%.
30893028	1	49	theme	Mexican	82:88	arg1	bats					102:105	Mexican free-tailed bats	82:105	Mexican free-tailed bats	82:105	nov., isolated from within a guano pile from a colony of Mexican free-tailed bats Tadarida brasiliensis.
30893028	4	50	theme	strain	462:467	arg1	TB-66T					469:474	strain TB-66T	462:474	strain TB-66T	462:474	The quinone system of strain TB-66T consisted predominantly of menaquinone MK-7.
30893028	9	51	theme	Filibacter	1020:1029	arg1	member					1000:1005	a member	998:1005	a member of the genus Filibacter	998:1029	On the basis of the genotypic and phenotypic properties it is clear that strain TB-66T represents a member of the genus Filibacter, but is distinct from the only other species in the genus, Filibacter limicola DSM 13886T.
30893028	12	52	theme	CIP	1222:1224	arg1	8866T					1251:1255	= CIP 111629T= LMG 30660T= CCM 8866T	1220:1255	= CIP 111629T= LMG 30660T= CCM 8866T	1220:1255	The type strain is TB-66T (= CIP 111629T= LMG 30660T= CCM 8866T).
30893028	12	52	theme	CIP	1222:1224	arg1	TB-66T					1212:1217	TB-66T	1212:1217	TB-66T (= CIP 111629T= LMG 30660T= CCM 8866T)	1212:1256	The type strain is TB-66T (= CIP 111629T= LMG 30660T= CCM 8866T).
30893028	1	53	theme	free-tailed	90:100	arg1	bats					102:105	Mexican free-tailed bats	82:105	Mexican free-tailed bats	82:105	nov., isolated from within a guano pile from a colony of Mexican free-tailed bats Tadarida brasiliensis.
30893028	9	54	theme	phenotypic	934:943	arg1	properties					945:954	the genotypic and phenotypic properties	916:954	the genotypic and phenotypic properties	916:954	On the basis of the genotypic and phenotypic properties it is clear that strain TB-66T represents a member of the genus Filibacter, but is distinct from the only other species in the genus, Filibacter limicola DSM 13886T.
30893028	7	55	theme	major	777:781	arg1	C15 					800:803	C15 	800:803	C15 	800:803	The major fatty acids were C15 : 0 anteiso, C16 : 0, and C16 : 1 ω7c.
30893028	7	55	theme	major	777:781	arg1	acids					789:793	The major fatty acids	773:793	The major fatty acids	773:793	The major fatty acids were C15 : 0 anteiso, C16 : 0, and C16 : 1 ω7c.
30893028	1	56	theme	bats	102:105	arg1	colony					72:77	a colony	70:77	a colony of Mexican free-tailed bats	70:105	nov., isolated from within a guano pile from a colony of Mexican free-tailed bats Tadarida brasiliensis.
30893028	9	57	theme	properties	945:954	arg1	clear					962:966	clear	962:966	clear	962:966	On the basis of the genotypic and phenotypic properties it is clear that strain TB-66T represents a member of the genus Filibacter, but is distinct from the only other species in the genus, Filibacter limicola DSM 13886T.
30893028	5	58	theme	unidentified	692:703	arg1	phospholipids					705:717	three unidentified phospholipids	686:717	three unidentified phospholipids	686:717	The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylserine and three unidentified phospholipids.
30893028	10	59	theme	name	1158:1161	arg1	sp					1184:1185	the name Filibacter tadaridae sp	1154:1185	the name Filibacter tadaridae sp	1154:1185	We propose a novel species with the name Filibacter tadaridae sp.
30893028	3	60	theme	%	418:418	arg1	similarity					399:408	a 16S rRNA gene sequence similarity	374:408	a 16S rRNA gene sequence similarity of 98.5 % to the type strain	374:437	On the basis of 16S rRNA gene sequence similarity comparisons, strain TB-66Tgrouped together with Filibacter limicola showing a 16S rRNA gene sequence similarity of 98.5 % to the type strain.
30893028	9	61	from	species	1068:1074	arg1	distinct					1039:1046	distinct	1039:1046	distinct	1039:1046	On the basis of the genotypic and phenotypic properties it is clear that strain TB-66T represents a member of the genus Filibacter, but is distinct from the only other species in the genus, Filibacter limicola DSM 13886T.
30893028	9	61	from	species	1068:1074	arg1	13886T					1114:1119	the genus, Filibacter limicola DSM 13886T	1079:1119	13886T	1114:1119	On the basis of the genotypic and phenotypic properties it is clear that strain TB-66T represents a member of the genus Filibacter, but is distinct from the only other species in the genus, Filibacter limicola DSM 13886T.
30893028	2	62	theme	guano	212:216	arg1	pile					200:203	a pile	198:203	a pile of bat guano in a cave of New Mexico, USA	198:245	A Gram-stain-positive, aerobic bacterium, TB-66T, was isolated from a pile of bat guano in a cave of New Mexico, USA.
30893028	6	63	theme	A4α	747:749	arg1	A11.33					764:769	A11.33	764:769	A11.33	764:769	The peptidoglycan type was A4α l-Lys-d-Glu (A11.33).
30893028	6	63	theme	A4α	747:749	arg1	type					738:741	The peptidoglycan type	720:741	The peptidoglycan type	720:741	The peptidoglycan type was A4α l-Lys-d-Glu (A11.33).
30893028	6	63	theme	A4α	747:749	arg1	l-Lys-d-Glu					751:761	A4α l-Lys-d-Glu	747:761	A4α l-Lys-d-Glu (A11.33)	747:770	The peptidoglycan type was A4α l-Lys-d-Glu (A11.33).
30893028	3	64	theme	rRNA	380:383	arg1	similarity					399:408	a 16S rRNA gene sequence similarity	374:408	a 16S rRNA gene sequence similarity of 98.5 % to the type strain	374:437	On the basis of 16S rRNA gene sequence similarity comparisons, strain TB-66Tgrouped together with Filibacter limicola showing a 16S rRNA gene sequence similarity of 98.5 % to the type strain.
30893028	3	65	theme	16S	264:266	arg1	comparisons					298:308	16S rRNA gene sequence similarity comparisons	264:308	16S rRNA gene sequence similarity comparisons	264:308	On the basis of 16S rRNA gene sequence similarity comparisons, strain TB-66Tgrouped together with Filibacter limicola showing a 16S rRNA gene sequence similarity of 98.5 % to the type strain.
30893028	2	66	theme	bat	208:210	arg1	guano					212:216	bat guano	208:216	bat guano	208:216	A Gram-stain-positive, aerobic bacterium, TB-66T, was isolated from a pile of bat guano in a cave of New Mexico, USA.
30893028	9	67	theme	other	1062:1066	arg1	species					1068:1074	the only other species	1053:1074	the only other species in the genus, Filibacter limicola DSM 13886T	1053:1119	On the basis of the genotypic and phenotypic properties it is clear that strain TB-66T represents a member of the genus Filibacter, but is distinct from the only other species in the genus, Filibacter limicola DSM 13886T.
29800418	10	0	theme	extracellular	1645:1657	arg1	processes					1666:1674	extracellular matrix processes	1645:1674	extracellular matrix processes	1645:1674	At the age of 14 d, a lower activity of cell cycle-related processes and a higher activity of extracellular matrix processes were observed in the jejunal mucosa of piglets supplemented with FOS compared with control piglets.
29800418	4	1	theme	microbial	640:648	arg1	structures					660:669	intestinal microbial community structures	629:669	intestinal microbial community structures	629:669	It is currently not known what the specificities are of intestinal microbial community structures in relation to the programming of the intestinal mucosal immune system and development of the intestinal barrier function.
29800418	10	2	theme	matrix	1659:1664	arg1	processes					1666:1674	extracellular matrix processes	1645:1674	extracellular matrix processes	1645:1674	At the age of 14 d, a lower activity of cell cycle-related processes and a higher activity of extracellular matrix processes were observed in the jejunal mucosa of piglets supplemented with FOS compared with control piglets.
29800418	4	3	theme	intestinal	765:774	arg1	function					784:791	the intestinal barrier function	761:791	the intestinal barrier function	761:791	It is currently not known what the specificities are of intestinal microbial community structures in relation to the programming of the intestinal mucosal immune system and development of the intestinal barrier function.
29800418	12	4	contain	had	2059:2061	arg1	piglets					2029:2035	piglets	2029:2035	piglets supplemented with FOS	2029:2057	Villi height and crypt depth in the jejunum were significantly different at day 25 between the experimental and control groups, where piglets supplemented with FOS had greater villi and deeper crypts.
29800418	12	4	contain	had	2059:2061	arg2	crypts					2088:2093	greater villi and deeper crypts	2063:2093	greater villi and deeper crypts	2063:2093	Villi height and crypt depth in the jejunum were significantly different at day 25 between the experimental and control groups, where piglets supplemented with FOS had greater villi and deeper crypts.
29800418	2	5	from	cells	379:383	arg1	mucosa					403:408	the intestinal mucosa	388:408	the intestinal mucosa	388:408	These processes depend heavily on close interactions between gut microbiota and host cells in the intestinal mucosa.
29800418	11	6	theme	processes	1824:1832	arg1	activity					1797:1804	the lower activity	1787:1804	the lower activity of immune-related processes in jejunal tissue	1787:1850	At day 25, the lower activity of immune-related processes in jejunal tissue was seen in piglets supplemented with FOS.
29800418	4	7	theme	intestinal	709:718	arg1	system					735:740	the intestinal mucosal immune system	705:740	the intestinal mucosal immune system	705:740	It is currently not known what the specificities are of intestinal microbial community structures in relation to the programming of the intestinal mucosal immune system and development of the intestinal barrier function.
29800418	5	8	theme	piglets	928:934	arg1	development					904:914	intestinal development	893:914	intestinal development of suckling piglets	893:934	The objective of the present study was to investigate the effects of a nutritional intervention on intestinal development of suckling piglets by daily oral administration of fructooligosaccharides (FOS) over a period of 12 d (days 2-14 of age).
29800418	12	9	from	depth	1918:1922	arg1	jejunum					1931:1937	the jejunum	1927:1937	the jejunum	1927:1937	Villi height and crypt depth in the jejunum were significantly different at day 25 between the experimental and control groups, where piglets supplemented with FOS had greater villi and deeper crypts.
29800418	4	10	theme	immune	728:733	arg1	system					735:740	the intestinal mucosal immune system	705:740	the intestinal mucosal immune system	705:740	It is currently not known what the specificities are of intestinal microbial community structures in relation to the programming of the intestinal mucosal immune system and development of the intestinal barrier function.
29800418	13	11	theme	significant	2185:2195	arg1	effects					2209:2215	significant bifidogenic effects	2185:2215	significant bifidogenic effects	2185:2215	We conclude that oral FOS administration during the early suckling period of piglets had significant bifidogenic effects on the microbiota in the colon and on gene expression in the jejunal mucosa by thus far unknown mechanisms.
29800418	2	12	theme	gut	355:357	arg1	microbiota					359:368	gut microbiota and host cells	355:383	microbiota	359:368	These processes depend heavily on close interactions between gut microbiota and host cells in the intestinal mucosa.
29800418	9	13	theme	significant	1423:1433	arg1	sets					1465:1468	significant differentially expressed gene sets	1423:1468	significant differentially expressed gene sets in mucosal tissues of the jejunum	1423:1502	In addition, significant differentially expressed gene sets in mucosal tissues of the jejunum were identified at both days 14 and 25 of age.
29800418	12	14	theme	crypt	1912:1916	arg1	depth					1918:1922	crypt depth	1912:1922	crypt depth	1912:1922	Villi height and crypt depth in the jejunum were significantly different at day 25 between the experimental and control groups, where piglets supplemented with FOS had greater villi and deeper crypts.
29800418	8	15	from	day	1402:1404	arg1	diversity					1389:1397	microbiota diversity	1378:1397	microbiota diversity at day 25	1378:1407	In the jejunum, significant changes were observed for microbiota composition at day 14, and microbiota diversity at day 25.
29800418	8	15	from	day	1402:1404	arg1	composition					1351:1361	microbiota composition	1340:1361	microbiota composition at day 14	1340:1371	In the jejunum, significant changes were observed for microbiota composition at day 14, and microbiota diversity at day 25.
29800418	9	16	theme	expressed	1450:1458	arg1	sets					1465:1468	significant differentially expressed gene sets	1423:1468	significant differentially expressed gene sets in mucosal tissues of the jejunum	1423:1502	In addition, significant differentially expressed gene sets in mucosal tissues of the jejunum were identified at both days 14 and 25 of age.
29800418	11	17	theme	immune-related	1809:1822	arg1	processes					1824:1832	immune-related processes	1809:1832	immune-related processes	1809:1832	At day 25, the lower activity of immune-related processes in jejunal tissue was seen in piglets supplemented with FOS.
29800418	10	18	theme	d	1568:1568	arg1	age					1558:1560	the age	1554:1560	the age of 14 d	1554:1568	At the age of 14 d, a lower activity of cell cycle-related processes and a higher activity of extracellular matrix processes were observed in the jejunal mucosa of piglets supplemented with FOS compared with control piglets.
29800418	5	19	dep	days	1020:1023	arg1	age					1033:1035	age	1033:1035	age	1033:1035	The objective of the present study was to investigate the effects of a nutritional intervention on intestinal development of suckling piglets by daily oral administration of fructooligosaccharides (FOS) over a period of 12 d (days 2-14 of age).
29800418	5	19	dep	days	1020:1023	arg1	2-14					1025:1028	2-14	1025:1028	2-14	1025:1028	The objective of the present study was to investigate the effects of a nutritional intervention on intestinal development of suckling piglets by daily oral administration of fructooligosaccharides (FOS) over a period of 12 d (days 2-14 of age).
29800418	9	20	theme	mucosal	1473:1479	arg1	tissues					1481:1487	mucosal tissues	1473:1487	mucosal tissues of the jejunum	1473:1502	In addition, significant differentially expressed gene sets in mucosal tissues of the jejunum were identified at both days 14 and 25 of age.
29800418	4	21	theme	function	784:791	arg1	programming					690:700	the programming	686:700	the programming of the intestinal mucosal immune system	686:740	It is currently not known what the specificities are of intestinal microbial community structures in relation to the programming of the intestinal mucosal immune system and development of the intestinal barrier function.
29800418	4	21	theme	function	784:791	arg1	development					746:756	development	746:756	development of the intestinal barrier function	746:791	It is currently not known what the specificities are of intestinal microbial community structures in relation to the programming of the intestinal mucosal immune system and development of the intestinal barrier function.
29800418	10	22	theme	jejunal	1697:1703	arg1	mucosa					1705:1710	the jejunal mucosa	1693:1710	the jejunal mucosa of piglets supplemented with FOS compared with control piglets	1693:1773	At the age of 14 d, a lower activity of cell cycle-related processes and a higher activity of extracellular matrix processes were observed in the jejunal mucosa of piglets supplemented with FOS compared with control piglets.
29800418	6	23	theme	microbiota	1046:1055	arg1	level					1067:1071	the microbiota community level	1042:1071	the microbiota community level	1042:1071	At the microbiota community level, a clear "bifidogenic" effect of the FOS administration was observed in the colon digesta at day 14.
29800418	6	24	located	observed	1133:1140	arg2	effect					1096:1101	a clear "bifidogenic" effect	1074:1101	a clear "bifidogenic" effect of the FOS administration	1074:1127	At the microbiota community level, a clear "bifidogenic" effect of the FOS administration was observed in the colon digesta at day 14.
29800418	6	24	located	observed	1133:1140	arg1	level					1067:1071	the microbiota community level	1042:1071	the microbiota community level	1042:1071	At the microbiota community level, a clear "bifidogenic" effect of the FOS administration was observed in the colon digesta at day 14.
29800418	6	24	located	observed	1133:1140	arg1	day					1166:1168	day 14	1166:1171	day 14	1166:1171	At the microbiota community level, a clear "bifidogenic" effect of the FOS administration was observed in the colon digesta at day 14.
29800418	6	24	located	observed	1133:1140	arg1	digesta					1155:1161	the colon digesta	1145:1161	the colon digesta	1145:1161	At the microbiota community level, a clear "bifidogenic" effect of the FOS administration was observed in the colon digesta at day 14.
29800418	5	25	theme	present	815:821	arg1	study					823:827	the present study	811:827	the present study	811:827	The objective of the present study was to investigate the effects of a nutritional intervention on intestinal development of suckling piglets by daily oral administration of fructooligosaccharides (FOS) over a period of 12 d (days 2-14 of age).
29800418	5	26	theme	intervention	877:888	arg1	effects					852:858	the effects	848:858	the effects of a nutritional intervention on intestinal development of suckling piglets	848:934	The objective of the present study was to investigate the effects of a nutritional intervention on intestinal development of suckling piglets by daily oral administration of fructooligosaccharides (FOS) over a period of 12 d (days 2-14 of age).
29800418	9	27	theme	jejunum	1496:1502	arg1	tissues					1481:1487	mucosal tissues	1473:1487	mucosal tissues of the jejunum	1473:1502	In addition, significant differentially expressed gene sets in mucosal tissues of the jejunum were identified at both days 14 and 25 of age.
29800418	5	28	theme	fructooligosaccharides	968:989	arg1	administration					950:963	daily oral administration	939:963	daily oral administration of fructooligosaccharides (FOS)	939:995	The objective of the present study was to investigate the effects of a nutritional intervention on intestinal development of suckling piglets by daily oral administration of fructooligosaccharides (FOS) over a period of 12 d (days 2-14 of age).
29800418	2	29	from	microbiota	359:368	arg1	mucosa					403:408	the intestinal mucosa	388:408	the intestinal mucosa	388:408	These processes depend heavily on close interactions between gut microbiota and host cells in the intestinal mucosa.
29800418	11	30	from	activity	1797:1804	arg1	tissue					1845:1850	jejunal tissue	1837:1850	jejunal tissue	1837:1850	At day 25, the lower activity of immune-related processes in jejunal tissue was seen in piglets supplemented with FOS.
29800418	12	31	theme	control	2007:2013	arg1	groups					2015:2020	the experimental and control groups	1986:2020	groups	2015:2020	Villi height and crypt depth in the jejunum were significantly different at day 25 between the experimental and control groups, where piglets supplemented with FOS had greater villi and deeper crypts.
29800418	10	32	theme	processes	1610:1618	arg1	activity					1579:1586	a lower activity	1571:1586	a lower activity of cell cycle-related processes	1571:1618	At the age of 14 d, a lower activity of cell cycle-related processes and a higher activity of extracellular matrix processes were observed in the jejunal mucosa of piglets supplemented with FOS compared with control piglets.
29800418	10	32	theme	processes	1610:1618	arg1	activity					1633:1640	a higher activity	1624:1640	a higher activity of extracellular matrix processes	1624:1674	At the age of 14 d, a lower activity of cell cycle-related processes and a higher activity of extracellular matrix processes were observed in the jejunal mucosa of piglets supplemented with FOS compared with control piglets.
29800418	8	33	theme	microbiota	1340:1349	arg1	composition					1351:1361	microbiota composition	1340:1361	microbiota composition at day 14	1340:1371	In the jejunum, significant changes were observed for microbiota composition at day 14, and microbiota diversity at day 25.
29800418	11	34	theme	jejunal	1837:1843	arg1	tissue					1845:1850	jejunal tissue	1837:1850	jejunal tissue	1837:1850	At day 25, the lower activity of immune-related processes in jejunal tissue was seen in piglets supplemented with FOS.
29800418	13	35	contain	had	2181:2183	arg1	administration					2122:2135	oral FOS administration	2113:2135	oral FOS administration during the early suckling period of piglets	2113:2179	We conclude that oral FOS administration during the early suckling period of piglets had significant bifidogenic effects on the microbiota in the colon and on gene expression in the jejunal mucosa by thus far unknown mechanisms.
29800418	13	35	contain	had	2181:2183	arg2	effects					2209:2215	significant bifidogenic effects	2185:2215	significant bifidogenic effects	2185:2215	We conclude that oral FOS administration during the early suckling period of piglets had significant bifidogenic effects on the microbiota in the colon and on gene expression in the jejunal mucosa by thus far unknown mechanisms.
29800418	1	36	from	importance	158:167	arg1	programming					193:203	programming the intestinal mucosal immune system and development of the intestinal barrier function	193:291	programming	193:203	Emerging knowledge shows the importance of early life events in programming the intestinal mucosal immune system and development of the intestinal barrier function.
29800418	1	36	from	importance	158:167	arg1	development					246:256	development	246:256	development of the intestinal barrier function	246:291	Emerging knowledge shows the importance of early life events in programming the intestinal mucosal immune system and development of the intestinal barrier function.
29800418	6	37	theme	"	1094:1094	arg1	effect					1096:1101	a clear "bifidogenic" effect	1074:1101	a clear "bifidogenic" effect of the FOS administration	1074:1127	At the microbiota community level, a clear "bifidogenic" effect of the FOS administration was observed in the colon digesta at day 14.
29800418	10	38	theme	lower	1573:1577	arg1	activity					1579:1586	a lower activity	1571:1586	a lower activity of cell cycle-related processes	1571:1618	At the age of 14 d, a lower activity of cell cycle-related processes and a higher activity of extracellular matrix processes were observed in the jejunal mucosa of piglets supplemented with FOS compared with control piglets.
29800418	0	39	theme	fructooligosaccharides	19:40	arg1	Supplementation					0:14	Supplementation	0:14	Supplementation of fructooligosaccharides to suckling piglets	0:60	Supplementation of fructooligosaccharides to suckling piglets affects intestinal microbiota colonization and immune development.
29800418	13	40	theme	suckling	2154:2161	arg1	period					2163:2168	the early suckling period	2144:2168	the early suckling period	2144:2168	We conclude that oral FOS administration during the early suckling period of piglets had significant bifidogenic effects on the microbiota in the colon and on gene expression in the jejunal mucosa by thus far unknown mechanisms.
29800418	9	41	theme	age	1546:1548	arg1	days					1528:1531	days 14 and 25	1528:1541	days	1528:1531	In addition, significant differentially expressed gene sets in mucosal tissues of the jejunum were identified at both days 14 and 25 of age.
29800418	1	42	theme	early	172:176	arg1	events					183:188	early life events	172:188	early life events	172:188	Emerging knowledge shows the importance of early life events in programming the intestinal mucosal immune system and development of the intestinal barrier function.
29800418	3	43	theme	intestinal	439:448	arg1	microbiota					450:459	the intestinal microbiota	435:459	the intestinal microbiota	435:459	In turn, development of the intestinal microbiota is largely dependent on available nutrients required for the specific microbial community structures to expand.
29800418	0	44	theme	microbiota	81:90	arg1	colonization					92:103	intestinal microbiota colonization	70:103	intestinal microbiota colonization	70:103	Supplementation of fructooligosaccharides to suckling piglets affects intestinal microbiota colonization and immune development.
29800418	10	45	theme	cycle-related	1596:1608	arg1	processes					1610:1618	cell cycle-related processes	1591:1618	cell cycle-related processes	1591:1618	At the age of 14 d, a lower activity of cell cycle-related processes and a higher activity of extracellular matrix processes were observed in the jejunal mucosa of piglets supplemented with FOS compared with control piglets.
29800418	3	46	theme	available	485:493	arg1	nutrients					495:503	available nutrients	485:503	available nutrients required for the specific microbial community structures to expand	485:570	In turn, development of the intestinal microbiota is largely dependent on available nutrients required for the specific microbial community structures to expand.
29800418	0	47	theme	suckling	45:52	arg1	piglets					54:60	suckling piglets	45:60	suckling piglets	45:60	Supplementation of fructooligosaccharides to suckling piglets affects intestinal microbiota colonization and immune development.
29800418	13	48	theme	oral	2113:2116	arg1	administration					2122:2135	oral FOS administration	2113:2135	oral FOS administration during the early suckling period of piglets	2113:2179	We conclude that oral FOS administration during the early suckling period of piglets had significant bifidogenic effects on the microbiota in the colon and on gene expression in the jejunal mucosa by thus far unknown mechanisms.
29800418	12	49	from	height	1901:1906	arg1	jejunum					1931:1937	the jejunum	1927:1937	the jejunum	1927:1937	Villi height and crypt depth in the jejunum were significantly different at day 25 between the experimental and control groups, where piglets supplemented with FOS had greater villi and deeper crypts.
29800418	7	50	from	mucosa	1278:1283	arg1	changes					1230:1236	significant changes	1218:1236	significant changes of local gene expression in the colonic mucosa	1218:1283	The former, however, did not translate into significant changes of local gene expression in the colonic mucosa.
29800418	7	51	theme	significant	1218:1228	arg1	changes					1230:1236	significant changes	1218:1236	significant changes of local gene expression in the colonic mucosa	1218:1283	The former, however, did not translate into significant changes of local gene expression in the colonic mucosa.
29800418	1	52	dep	programming	193:203	arg1	system					235:240	the intestinal mucosal immune system	205:240	the intestinal mucosal immune system	205:240	Emerging knowledge shows the importance of early life events in programming the intestinal mucosal immune system and development of the intestinal barrier function.
29800418	3	53	theme	microbial	531:539	arg1	structures					551:560	the specific microbial community structures	518:560	the specific microbial community structures to expand	518:570	In turn, development of the intestinal microbiota is largely dependent on available nutrients required for the specific microbial community structures to expand.
29800418	13	54	theme	unknown	2305:2311	arg1	mechanisms					2313:2322	thus far unknown mechanisms	2296:2322	thus far unknown mechanisms	2296:2322	We conclude that oral FOS administration during the early suckling period of piglets had significant bifidogenic effects on the microbiota in the colon and on gene expression in the jejunal mucosa by thus far unknown mechanisms.
29800418	7	55	theme	colonic	1270:1276	arg1	mucosa					1278:1283	the colonic mucosa	1266:1283	the colonic mucosa	1266:1283	The former, however, did not translate into significant changes of local gene expression in the colonic mucosa.
29800418	10	56	theme	control	1759:1765	arg1	piglets					1767:1773	control piglets	1759:1773	control piglets	1759:1773	At the age of 14 d, a lower activity of cell cycle-related processes and a higher activity of extracellular matrix processes were observed in the jejunal mucosa of piglets supplemented with FOS compared with control piglets.
29800418	11	57	theme	lower	1791:1795	arg1	activity					1797:1804	the lower activity	1787:1804	the lower activity of immune-related processes in jejunal tissue	1787:1850	At day 25, the lower activity of immune-related processes in jejunal tissue was seen in piglets supplemented with FOS.
29800418	2	58	theme	host	374:377	arg1	cells					379:383	gut microbiota and host cells	355:383	cells	379:383	These processes depend heavily on close interactions between gut microbiota and host cells in the intestinal mucosa.
29800418	5	59	theme	age	1033:1035	arg1	age					1033:1035	age	1033:1035	age	1033:1035	The objective of the present study was to investigate the effects of a nutritional intervention on intestinal development of suckling piglets by daily oral administration of fructooligosaccharides (FOS) over a period of 12 d (days 2-14 of age).
29800418	5	59	theme	age	1033:1035	arg1	2-14					1025:1028	2-14	1025:1028	2-14	1025:1028	The objective of the present study was to investigate the effects of a nutritional intervention on intestinal development of suckling piglets by daily oral administration of fructooligosaccharides (FOS) over a period of 12 d (days 2-14 of age).
29800418	1	60	theme	life	178:181	arg1	events					183:188	early life events	172:188	early life events	172:188	Emerging knowledge shows the importance of early life events in programming the intestinal mucosal immune system and development of the intestinal barrier function.
29800418	4	61	theme	community	650:658	arg1	structures					660:669	intestinal microbial community structures	629:669	intestinal microbial community structures	629:669	It is currently not known what the specificities are of intestinal microbial community structures in relation to the programming of the intestinal mucosal immune system and development of the intestinal barrier function.
29800418	13	62	from	expression	2260:2269	arg1	mucosa					2286:2291	the jejunal mucosa	2274:2291	the jejunal mucosa by thus far unknown mechanisms	2274:2322	We conclude that oral FOS administration during the early suckling period of piglets had significant bifidogenic effects on the microbiota in the colon and on gene expression in the jejunal mucosa by thus far unknown mechanisms.
29800418	1	63	theme	intestinal	265:274	arg1	function					284:291	the intestinal barrier function	261:291	the intestinal barrier function	261:291	Emerging knowledge shows the importance of early life events in programming the intestinal mucosal immune system and development of the intestinal barrier function.
29800418	8	64	located	observed	1327:1334	arg2	changes					1314:1320	significant changes	1302:1320	significant changes	1302:1320	In the jejunum, significant changes were observed for microbiota composition at day 14, and microbiota diversity at day 25.
29800418	8	64	located	observed	1327:1334	arg1	jejunum					1293:1299	the jejunum	1289:1299	the jejunum	1289:1299	In the jejunum, significant changes were observed for microbiota composition at day 14, and microbiota diversity at day 25.
29800418	6	65	theme	FOS	1110:1112	arg1	administration					1114:1127	the FOS administration	1106:1127	the FOS administration	1106:1127	At the microbiota community level, a clear "bifidogenic" effect of the FOS administration was observed in the colon digesta at day 14.
29800418	13	66	theme	piglets	2173:2179	arg1	administration					2122:2135	oral FOS administration	2113:2135	oral FOS administration during the early suckling period of piglets	2113:2179	We conclude that oral FOS administration during the early suckling period of piglets had significant bifidogenic effects on the microbiota in the colon and on gene expression in the jejunal mucosa by thus far unknown mechanisms.
29800418	4	67	from	structures	660:669	arg1	relation					674:681	relation	674:681	relation to the programming of the intestinal mucosal immune system and development of the intestinal barrier function	674:791	It is currently not known what the specificities are of intestinal microbial community structures in relation to the programming of the intestinal mucosal immune system and development of the intestinal barrier function.
29800418	2	68	theme	intestinal	392:401	arg1	mucosa					403:408	the intestinal mucosa	388:408	the intestinal mucosa	388:408	These processes depend heavily on close interactions between gut microbiota and host cells in the intestinal mucosa.
29800418	8	69	from	day	1366:1368	arg1	diversity					1389:1397	microbiota diversity	1378:1397	microbiota diversity at day 25	1378:1407	In the jejunum, significant changes were observed for microbiota composition at day 14, and microbiota diversity at day 25.
29800418	8	69	from	day	1366:1368	arg1	composition					1351:1361	microbiota composition	1340:1361	microbiota composition at day 14	1340:1371	In the jejunum, significant changes were observed for microbiota composition at day 14, and microbiota diversity at day 25.
29800418	1	70	theme	function	284:291	arg1	programming					193:203	programming the intestinal mucosal immune system and development of the intestinal barrier function	193:291	programming	193:203	Emerging knowledge shows the importance of early life events in programming the intestinal mucosal immune system and development of the intestinal barrier function.
29800418	1	70	theme	function	284:291	arg1	development					246:256	development	246:256	development of the intestinal barrier function	246:291	Emerging knowledge shows the importance of early life events in programming the intestinal mucosal immune system and development of the intestinal barrier function.
29800418	7	71	from	changes	1230:1236	arg1	mucosa					1278:1283	the colonic mucosa	1266:1283	the colonic mucosa	1266:1283	The former, however, did not translate into significant changes of local gene expression in the colonic mucosa.
29800418	2	72	theme	close	328:332	arg1	interactions					334:345	close interactions	328:345	close interactions between gut microbiota and host cells in the intestinal mucosa	328:408	These processes depend heavily on close interactions between gut microbiota and host cells in the intestinal mucosa.
29800418	4	73	theme	intestinal	629:638	arg1	structures					660:669	intestinal microbial community structures	629:669	intestinal microbial community structures	629:669	It is currently not known what the specificities are of intestinal microbial community structures in relation to the programming of the intestinal mucosal immune system and development of the intestinal barrier function.
29800418	13	74	theme	gene	2255:2258	arg1	expression					2260:2269	gene expression	2255:2269	gene expression in the jejunal mucosa by thus far unknown mechanisms	2255:2322	We conclude that oral FOS administration during the early suckling period of piglets had significant bifidogenic effects on the microbiota in the colon and on gene expression in the jejunal mucosa by thus far unknown mechanisms.
29800418	5	75	theme	suckling	919:926	arg1	piglets					928:934	suckling piglets	919:934	suckling piglets	919:934	The objective of the present study was to investigate the effects of a nutritional intervention on intestinal development of suckling piglets by daily oral administration of fructooligosaccharides (FOS) over a period of 12 d (days 2-14 of age).
29800418	7	76	from	expression	1252:1261	arg1	mucosa					1278:1283	the colonic mucosa	1266:1283	the colonic mucosa	1266:1283	The former, however, did not translate into significant changes of local gene expression in the colonic mucosa.
29800418	1	77	theme	intestinal	209:218	arg1	system					235:240	the intestinal mucosal immune system	205:240	the intestinal mucosal immune system	205:240	Emerging knowledge shows the importance of early life events in programming the intestinal mucosal immune system and development of the intestinal barrier function.
29800418	12	78	theme	Villi	1895:1899	arg1	height					1901:1906	Villi height	1895:1906	Villi height	1895:1906	Villi height and crypt depth in the jejunum were significantly different at day 25 between the experimental and control groups, where piglets supplemented with FOS had greater villi and deeper crypts.
29800418	4	79	theme	mucosal	720:726	arg1	system					735:740	the intestinal mucosal immune system	705:740	the intestinal mucosal immune system	705:740	It is currently not known what the specificities are of intestinal microbial community structures in relation to the programming of the intestinal mucosal immune system and development of the intestinal barrier function.
29800418	1	80	theme	immune	228:233	arg1	system					235:240	the intestinal mucosal immune system	205:240	the intestinal mucosal immune system	205:240	Emerging knowledge shows the importance of early life events in programming the intestinal mucosal immune system and development of the intestinal barrier function.
29800418	10	81	theme	piglets	1715:1721	arg1	mucosa					1705:1710	the jejunal mucosa	1693:1710	the jejunal mucosa of piglets supplemented with FOS compared with control piglets	1693:1773	At the age of 14 d, a lower activity of cell cycle-related processes and a higher activity of extracellular matrix processes were observed in the jejunal mucosa of piglets supplemented with FOS compared with control piglets.
29800418	6	82	theme	colon	1149:1153	arg1	digesta					1155:1161	the colon digesta	1145:1161	the colon digesta	1145:1161	At the microbiota community level, a clear "bifidogenic" effect of the FOS administration was observed in the colon digesta at day 14.
29800418	1	83	theme	mucosal	220:226	arg1	system					235:240	the intestinal mucosal immune system	205:240	the intestinal mucosal immune system	205:240	Emerging knowledge shows the importance of early life events in programming the intestinal mucosal immune system and development of the intestinal barrier function.
29800418	9	84	theme	gene	1460:1463	arg1	sets					1465:1468	significant differentially expressed gene sets	1423:1468	significant differentially expressed gene sets in mucosal tissues of the jejunum	1423:1502	In addition, significant differentially expressed gene sets in mucosal tissues of the jejunum were identified at both days 14 and 25 of age.
29800418	13	85	theme	bifidogenic	2197:2207	arg1	effects					2209:2215	significant bifidogenic effects	2185:2215	significant bifidogenic effects	2185:2215	We conclude that oral FOS administration during the early suckling period of piglets had significant bifidogenic effects on the microbiota in the colon and on gene expression in the jejunal mucosa by thus far unknown mechanisms.
29800418	12	86	theme	experimental	1990:2001	arg1	groups					2015:2020	the experimental and control groups	1986:2020	groups	2015:2020	Villi height and crypt depth in the jejunum were significantly different at day 25 between the experimental and control groups, where piglets supplemented with FOS had greater villi and deeper crypts.
29800418	5	87	theme	daily	939:943	arg1	administration					950:963	daily oral administration	939:963	daily oral administration of fructooligosaccharides (FOS)	939:995	The objective of the present study was to investigate the effects of a nutritional intervention on intestinal development of suckling piglets by daily oral administration of fructooligosaccharides (FOS) over a period of 12 d (days 2-14 of age).
29800418	6	88	theme	community	1057:1065	arg1	level					1067:1071	the microbiota community level	1042:1071	the microbiota community level	1042:1071	At the microbiota community level, a clear "bifidogenic" effect of the FOS administration was observed in the colon digesta at day 14.
29800418	4	89	theme	barrier	776:782	arg1	function					784:791	the intestinal barrier function	761:791	the intestinal barrier function	761:791	It is currently not known what the specificities are of intestinal microbial community structures in relation to the programming of the intestinal mucosal immune system and development of the intestinal barrier function.
29800418	10	90	theme	higher	1626:1631	arg1	activity					1633:1640	a higher activity	1624:1640	a higher activity of extracellular matrix processes	1624:1674	At the age of 14 d, a lower activity of cell cycle-related processes and a higher activity of extracellular matrix processes were observed in the jejunal mucosa of piglets supplemented with FOS compared with control piglets.
29800418	5	91	theme	oral	945:948	arg1	administration					950:963	daily oral administration	939:963	daily oral administration of fructooligosaccharides (FOS)	939:995	The objective of the present study was to investigate the effects of a nutritional intervention on intestinal development of suckling piglets by daily oral administration of fructooligosaccharides (FOS) over a period of 12 d (days 2-14 of age).
29800418	4	92	theme	system	735:740	arg1	programming					690:700	the programming	686:700	the programming of the intestinal mucosal immune system	686:740	It is currently not known what the specificities are of intestinal microbial community structures in relation to the programming of the intestinal mucosal immune system and development of the intestinal barrier function.
29800418	4	92	theme	system	735:740	arg1	development					746:756	development	746:756	development of the intestinal barrier function	746:791	It is currently not known what the specificities are of intestinal microbial community structures in relation to the programming of the intestinal mucosal immune system and development of the intestinal barrier function.
29800418	0	93	theme	immune	109:114	arg1	development					116:126	immune development	109:126	immune development	109:126	Supplementation of fructooligosaccharides to suckling piglets affects intestinal microbiota colonization and immune development.
29800418	5	94	from	effects	852:858	arg1	development					904:914	intestinal development	893:914	intestinal development of suckling piglets	893:934	The objective of the present study was to investigate the effects of a nutritional intervention on intestinal development of suckling piglets by daily oral administration of fructooligosaccharides (FOS) over a period of 12 d (days 2-14 of age).
29800418	5	95	theme	study	823:827	arg1	objective					798:806	The objective	794:806	The objective of the present study	794:827	The objective of the present study was to investigate the effects of a nutritional intervention on intestinal development of suckling piglets by daily oral administration of fructooligosaccharides (FOS) over a period of 12 d (days 2-14 of age).
29800418	12	96	from	day	1971:1973	arg1	different					1958:1966	different	1958:1966	different	1958:1966	Villi height and crypt depth in the jejunum were significantly different at day 25 between the experimental and control groups, where piglets supplemented with FOS had greater villi and deeper crypts.
29800418	1	97	theme	events	183:188	arg1	importance					158:167	the importance	154:167	the importance of early life events in programming the intestinal mucosal immune system and development of the intestinal barrier function	154:291	Emerging knowledge shows the importance of early life events in programming the intestinal mucosal immune system and development of the intestinal barrier function.
29800418	8	98	theme	significant	1302:1312	arg1	changes					1314:1320	significant changes	1302:1320	significant changes	1302:1320	In the jejunum, significant changes were observed for microbiota composition at day 14, and microbiota diversity at day 25.
29800418	5	99	theme	d	1017:1017	arg1	period					1004:1009	a period	1002:1009	a period of 12 d (days 2-14 of age)	1002:1036	The objective of the present study was to investigate the effects of a nutritional intervention on intestinal development of suckling piglets by daily oral administration of fructooligosaccharides (FOS) over a period of 12 d (days 2-14 of age).
29800418	3	100	theme	specific	522:529	arg1	structures					551:560	the specific microbial community structures	518:560	the specific microbial community structures to expand	518:570	In turn, development of the intestinal microbiota is largely dependent on available nutrients required for the specific microbial community structures to expand.
29800418	10	101	located	observed	1681:1688	arg1	age					1558:1560	the age	1554:1560	the age of 14 d	1554:1568	At the age of 14 d, a lower activity of cell cycle-related processes and a higher activity of extracellular matrix processes were observed in the jejunal mucosa of piglets supplemented with FOS compared with control piglets.
29800418	10	101	located	observed	1681:1688	arg2	activity					1579:1586	a lower activity	1571:1586	a lower activity of cell cycle-related processes	1571:1618	At the age of 14 d, a lower activity of cell cycle-related processes and a higher activity of extracellular matrix processes were observed in the jejunal mucosa of piglets supplemented with FOS compared with control piglets.
29800418	10	101	located	observed	1681:1688	arg1	mucosa					1705:1710	the jejunal mucosa	1693:1710	the jejunal mucosa of piglets supplemented with FOS compared with control piglets	1693:1773	At the age of 14 d, a lower activity of cell cycle-related processes and a higher activity of extracellular matrix processes were observed in the jejunal mucosa of piglets supplemented with FOS compared with control piglets.
29800418	10	101	located	observed	1681:1688	arg2	activity					1633:1640	a higher activity	1624:1640	a higher activity of extracellular matrix processes	1624:1674	At the age of 14 d, a lower activity of cell cycle-related processes and a higher activity of extracellular matrix processes were observed in the jejunal mucosa of piglets supplemented with FOS compared with control piglets.
29800418	3	102	from	turn	414:417	arg1	dependent					472:480	dependent	472:480	dependent	472:480	In turn, development of the intestinal microbiota is largely dependent on available nutrients required for the specific microbial community structures to expand.
29800418	5	103	theme	intestinal	893:902	arg1	development					904:914	intestinal development	893:914	intestinal development of suckling piglets	893:934	The objective of the present study was to investigate the effects of a nutritional intervention on intestinal development of suckling piglets by daily oral administration of fructooligosaccharides (FOS) over a period of 12 d (days 2-14 of age).
29800418	6	104	theme	bifidogenic	1083:1093	arg1	effect					1096:1101	a clear "bifidogenic" effect	1074:1101	a clear "bifidogenic" effect of the FOS administration	1074:1127	At the microbiota community level, a clear "bifidogenic" effect of the FOS administration was observed in the colon digesta at day 14.
29800418	5	105	theme	nutritional	865:875	arg1	intervention					877:888	a nutritional intervention	863:888	a nutritional intervention	863:888	The objective of the present study was to investigate the effects of a nutritional intervention on intestinal development of suckling piglets by daily oral administration of fructooligosaccharides (FOS) over a period of 12 d (days 2-14 of age).
29800418	13	106	theme	early	2148:2152	arg1	period					2163:2168	the early suckling period	2144:2168	the early suckling period	2144:2168	We conclude that oral FOS administration during the early suckling period of piglets had significant bifidogenic effects on the microbiota in the colon and on gene expression in the jejunal mucosa by thus far unknown mechanisms.
29800418	1	107	theme	Emerging	129:136	arg1	knowledge					138:146	Emerging knowledge	129:146	Emerging knowledge	129:146	Emerging knowledge shows the importance of early life events in programming the intestinal mucosal immune system and development of the intestinal barrier function.
29800418	6	108	theme	clear	1076:1080	arg1	effect					1096:1101	a clear "bifidogenic" effect	1074:1101	a clear "bifidogenic" effect of the FOS administration	1074:1127	At the microbiota community level, a clear "bifidogenic" effect of the FOS administration was observed in the colon digesta at day 14.
29800418	6	109	theme	administration	1114:1127	arg1	effect					1096:1101	a clear "bifidogenic" effect	1074:1101	a clear "bifidogenic" effect of the FOS administration	1074:1127	At the microbiota community level, a clear "bifidogenic" effect of the FOS administration was observed in the colon digesta at day 14.
29800418	3	110	theme	microbiota	450:459	arg1	development					420:430	development	420:430	development of the intestinal microbiota	420:459	In turn, development of the intestinal microbiota is largely dependent on available nutrients required for the specific microbial community structures to expand.
29800418	10	111	theme	cell	1591:1594	arg1	processes					1610:1618	cell cycle-related processes	1591:1618	cell cycle-related processes	1591:1618	At the age of 14 d, a lower activity of cell cycle-related processes and a higher activity of extracellular matrix processes were observed in the jejunal mucosa of piglets supplemented with FOS compared with control piglets.
29800418	0	112	theme	intestinal	70:79	arg1	colonization					92:103	intestinal microbiota colonization	70:103	intestinal microbiota colonization	70:103	Supplementation of fructooligosaccharides to suckling piglets affects intestinal microbiota colonization and immune development.
29800418	13	113	theme	FOS	2118:2120	arg1	administration					2122:2135	oral FOS administration	2113:2135	oral FOS administration during the early suckling period of piglets	2113:2179	We conclude that oral FOS administration during the early suckling period of piglets had significant bifidogenic effects on the microbiota in the colon and on gene expression in the jejunal mucosa by thus far unknown mechanisms.
29800418	9	114	from	sets	1465:1468	arg1	tissues					1481:1487	mucosal tissues	1473:1487	mucosal tissues of the jejunum	1473:1502	In addition, significant differentially expressed gene sets in mucosal tissues of the jejunum were identified at both days 14 and 25 of age.
29800418	12	115	dep	villi	2071:2075	arg1	greater					2063:2069	greater	2063:2069	greater	2063:2069	Villi height and crypt depth in the jejunum were significantly different at day 25 between the experimental and control groups, where piglets supplemented with FOS had greater villi and deeper crypts.
29800418	8	116	theme	microbiota	1378:1387	arg1	diversity					1389:1397	microbiota diversity	1378:1397	microbiota diversity at day 25	1378:1407	In the jejunum, significant changes were observed for microbiota composition at day 14, and microbiota diversity at day 25.
29800418	13	117	from	microbiota	2224:2233	arg1	colon					2242:2246	the colon	2238:2246	the colon	2238:2246	We conclude that oral FOS administration during the early suckling period of piglets had significant bifidogenic effects on the microbiota in the colon and on gene expression in the jejunal mucosa by thus far unknown mechanisms.
29800418	3	118	theme	community	541:549	arg1	structures					551:560	the specific microbial community structures	518:560	the specific microbial community structures to expand	518:570	In turn, development of the intestinal microbiota is largely dependent on available nutrients required for the specific microbial community structures to expand.
29800418	7	119	theme	expression	1252:1261	arg1	changes					1230:1236	significant changes	1218:1236	significant changes of local gene expression in the colonic mucosa	1218:1283	The former, however, did not translate into significant changes of local gene expression in the colonic mucosa.
29800418	12	120	theme	villi	2071:2075	arg1	crypts					2088:2093	greater villi and deeper crypts	2063:2093	greater villi and deeper crypts	2063:2093	Villi height and crypt depth in the jejunum were significantly different at day 25 between the experimental and control groups, where piglets supplemented with FOS had greater villi and deeper crypts.
29800418	7	121	theme	gene	1247:1250	arg1	expression					1252:1261	local gene expression	1241:1261	local gene expression in the colonic mucosa	1241:1283	The former, however, did not translate into significant changes of local gene expression in the colonic mucosa.
29800418	13	122	theme	jejunal	2278:2284	arg1	mucosa					2286:2291	the jejunal mucosa	2274:2291	the jejunal mucosa by thus far unknown mechanisms	2274:2322	We conclude that oral FOS administration during the early suckling period of piglets had significant bifidogenic effects on the microbiota in the colon and on gene expression in the jejunal mucosa by thus far unknown mechanisms.
29800418	7	123	theme	local	1241:1245	arg1	expression					1252:1261	local gene expression	1241:1261	local gene expression in the colonic mucosa	1241:1283	The former, however, did not translate into significant changes of local gene expression in the colonic mucosa.
29800418	12	124	theme	deeper	2081:2086	arg1	crypts					2088:2093	greater villi and deeper crypts	2063:2093	greater villi and deeper crypts	2063:2093	Villi height and crypt depth in the jejunum were significantly different at day 25 between the experimental and control groups, where piglets supplemented with FOS had greater villi and deeper crypts.
29800418	3	125	from	dependent	472:480	arg1	turn					414:417	turn	414:417	turn	414:417	In turn, development of the intestinal microbiota is largely dependent on available nutrients required for the specific microbial community structures to expand.
29800418	10	126	theme	processes	1666:1674	arg1	activity					1579:1586	a lower activity	1571:1586	a lower activity of cell cycle-related processes	1571:1618	At the age of 14 d, a lower activity of cell cycle-related processes and a higher activity of extracellular matrix processes were observed in the jejunal mucosa of piglets supplemented with FOS compared with control piglets.
29800418	10	126	theme	processes	1666:1674	arg1	activity					1633:1640	a higher activity	1624:1640	a higher activity of extracellular matrix processes	1624:1674	At the age of 14 d, a lower activity of cell cycle-related processes and a higher activity of extracellular matrix processes were observed in the jejunal mucosa of piglets supplemented with FOS compared with control piglets.
29800418	1	127	theme	barrier	276:282	arg1	function					284:291	the intestinal barrier function	261:291	the intestinal barrier function	261:291	Emerging knowledge shows the importance of early life events in programming the intestinal mucosal immune system and development of the intestinal barrier function.
31038452	11	0	theme	lipid	1193:1197	arg1	profile					1199:1205	The polar lipid profile	1183:1205	The polar lipid profile	1183:1205	The polar lipid profile contained unidentified phosphoglycolipids and unidentified glycolipids.
31038452	2	1	theme	aerobic	110:116	arg1	bacterium					148:156	A Gram-stain-negative, aerobic, non-motile and coccus-shaped bacterium	87:156	A Gram-stain-negative, aerobic, non-motile and coccus-shaped bacterium	87:156	A Gram-stain-negative, aerobic, non-motile and coccus-shaped bacterium, designated strain FeSDHB5-19T, was isolated from a biofilm sample collected from a radioactive thermal spring (Budapest, Hungary), after exposure to 5 kGy gamma radiation.
31038452	11	2	theme	unidentified	1253:1264	arg1	glycolipids					1266:1276	unidentified glycolipids	1253:1276	unidentified glycolipids	1253:1276	The polar lipid profile contained unidentified phosphoglycolipids and unidentified glycolipids.
31038452	10	3	theme	Deinococcus	1170:1180	arg1	typical					1149:1155	typical	1149:1155	typical	1149:1155	Strain FeSDHB5-19T possessed MK-8 as the predominant respiratory quinone, typical of the genus Deinococcus.
31038452	1	4	from	spring	68:73	arg1	Hungary					78:84	Hungary	78:84	Hungary	78:84	nov., isolated from a radioactive thermal spring in Hungary.
31038452	4	5	theme	gene	530:533	arg1	%					610:610	93.0 % or lower	605:619	93.0 % or lower	605:619	The 16S rRNA gene sequence similarity to type strains of other Deinococcus species were 93.0 % or lower.
31038452	4	5	theme	gene	530:533	arg1	similarity					544:553	The 16S rRNA gene sequence similarity	517:553	The 16S rRNA gene sequence similarity to type strains of other Deinococcus species	517:598	The 16S rRNA gene sequence similarity to type strains of other Deinococcus species were 93.0 % or lower.
31038452	5	6	theme	63.9 mol	698:705	arg1	%					706:706	63.9 mol%	698:706	63.9 mol%	698:706	The DNA G+C content of the draft genome sequence, consisting of 3.9 Mb, was 63.9 mol%.
31038452	5	6	theme	63.9 mol	698:705	arg1	G+C content					630:640	The DNA G+C content	622:640	The DNA G+C content of the draft genome sequence, consisting of 3.9 Mb,	622:692	The DNA G+C content of the draft genome sequence, consisting of 3.9 Mb, was 63.9 mol%.
31038452	2	7	theme	gamma	314:318	arg1	radiation					320:328	5 kGy gamma radiation	308:328	5 kGy gamma radiation	308:328	A Gram-stain-negative, aerobic, non-motile and coccus-shaped bacterium, designated strain FeSDHB5-19T, was isolated from a biofilm sample collected from a radioactive thermal spring (Budapest, Hungary), after exposure to 5 kGy gamma radiation.
31038452	4	8	theme	rRNA	525:528	arg1	%					610:610	93.0 % or lower	605:619	93.0 % or lower	605:619	The 16S rRNA gene sequence similarity to type strains of other Deinococcus species were 93.0 % or lower.
31038452	4	8	theme	rRNA	525:528	arg1	similarity					544:553	The 16S rRNA gene sequence similarity	517:553	The 16S rRNA gene sequence similarity to type strains of other Deinococcus species	517:598	The 16S rRNA gene sequence similarity to type strains of other Deinococcus species were 93.0 % or lower.
31038452	5	9	theme	sequence	662:669	arg1	%					706:706	63.9 mol%	698:706	63.9 mol%	698:706	The DNA G+C content of the draft genome sequence, consisting of 3.9 Mb, was 63.9 mol%.
31038452	5	9	theme	sequence	662:669	arg1	G+C content					630:640	The DNA G+C content	622:640	The DNA G+C content of the draft genome sequence, consisting of 3.9 Mb,	622:692	The DNA G+C content of the draft genome sequence, consisting of 3.9 Mb, was 63.9 mol%.
31038452	2	10	theme	Gram-stain-negative	89:107	arg1	bacterium					148:156	A Gram-stain-negative, aerobic, non-motile and coccus-shaped bacterium	87:156	A Gram-stain-negative, aerobic, non-motile and coccus-shaped bacterium	87:156	A Gram-stain-negative, aerobic, non-motile and coccus-shaped bacterium, designated strain FeSDHB5-19T, was isolated from a biofilm sample collected from a radioactive thermal spring (Budapest, Hungary), after exposure to 5 kGy gamma radiation.
31038452	13	11	theme	name	1540:1543	arg1	sp					1566:1567	the name Deinococcusfonticola sp	1536:1567	the name Deinococcusfonticola sp	1536:1567	According to its genotypic, phenotypic and chemotaxonomic characteristics, strain FeSDHB5-19T represents a novel species in the genus Deinococcus, for which the name Deinococcusfonticola sp.
31038452	13	12	theme	phenotypic	1407:1416	arg1	characteristics					1437:1451	its genotypic, phenotypic and chemotaxonomic characteristics	1392:1451	its genotypic, phenotypic and chemotaxonomic characteristics	1392:1451	According to its genotypic, phenotypic and chemotaxonomic characteristics, strain FeSDHB5-19T represents a novel species in the genus Deinococcus, for which the name Deinococcusfonticola sp.
31038452	13	13	theme	chemotaxonomic	1422:1435	arg1	characteristics					1437:1451	its genotypic, phenotypic and chemotaxonomic characteristics	1392:1451	its genotypic, phenotypic and chemotaxonomic characteristics	1392:1451	According to its genotypic, phenotypic and chemotaxonomic characteristics, strain FeSDHB5-19T represents a novel species in the genus Deinococcus, for which the name Deinococcusfonticola sp.
31038452	8	14	theme	cell-wall	959:967	arg1	type					983:986	The cell-wall peptidoglycan type	955:986	The cell-wall peptidoglycan type	955:986	The cell-wall peptidoglycan type was A3β l-Orn-Gly1-2.
31038452	8	14	theme	cell-wall	959:967	arg1	l-Orn-Gly1-2					996:1007	A3β l-Orn-Gly1-2	992:1007	A3β l-Orn-Gly1-2	992:1007	The cell-wall peptidoglycan type was A3β l-Orn-Gly1-2.
31038452	13	15	theme	Deinococcusfonticola	1545:1564	arg1	sp					1566:1567	the name Deinococcusfonticola sp	1536:1567	the name Deinococcusfonticola sp	1536:1567	According to its genotypic, phenotypic and chemotaxonomic characteristics, strain FeSDHB5-19T represents a novel species in the genus Deinococcus, for which the name Deinococcusfonticola sp.
31038452	1	16	theme	radioactive	48:58	arg1	spring					68:73	a radioactive thermal spring	46:73	a radioactive thermal spring in Hungary	46:84	nov., isolated from a radioactive thermal spring in Hungary.
31038452	4	17	theme	sequence	535:542	arg1	%					610:610	93.0 % or lower	605:619	93.0 % or lower	605:619	The 16S rRNA gene sequence similarity to type strains of other Deinococcus species were 93.0 % or lower.
31038452	4	17	theme	sequence	535:542	arg1	similarity					544:553	The 16S rRNA gene sequence similarity	517:553	The 16S rRNA gene sequence similarity to type strains of other Deinococcus species	517:598	The 16S rRNA gene sequence similarity to type strains of other Deinococcus species were 93.0 % or lower.
31038452	2	18	theme	coccus-shaped	134:146	arg1	bacterium					148:156	A Gram-stain-negative, aerobic, non-motile and coccus-shaped bacterium	87:156	A Gram-stain-negative, aerobic, non-motile and coccus-shaped bacterium	87:156	A Gram-stain-negative, aerobic, non-motile and coccus-shaped bacterium, designated strain FeSDHB5-19T, was isolated from a biofilm sample collected from a radioactive thermal spring (Budapest, Hungary), after exposure to 5 kGy gamma radiation.
31038452	13	19	theme	genus	1507:1511	arg1	Deinococcus					1513:1523	the genus Deinococcus	1503:1523	the genus Deinococcus	1503:1523	According to its genotypic, phenotypic and chemotaxonomic characteristics, strain FeSDHB5-19T represents a novel species in the genus Deinococcus, for which the name Deinococcusfonticola sp.
31038452	4	20	theme	other	574:578	arg1	species					592:598	other Deinococcus species	574:598	other Deinococcus species	574:598	The 16S rRNA gene sequence similarity to type strains of other Deinococcus species were 93.0 % or lower.
31038452	1	21	theme	thermal	60:66	arg1	spring					68:73	a radioactive thermal spring	46:73	a radioactive thermal spring in Hungary	46:84	nov., isolated from a radioactive thermal spring in Hungary.
31038452	2	22	attach	isolated	194:201	arg2	bacterium					148:156	A Gram-stain-negative, aerobic, non-motile and coccus-shaped bacterium	87:156	A Gram-stain-negative, aerobic, non-motile and coccus-shaped bacterium	87:156	A Gram-stain-negative, aerobic, non-motile and coccus-shaped bacterium, designated strain FeSDHB5-19T, was isolated from a biofilm sample collected from a radioactive thermal spring (Budapest, Hungary), after exposure to 5 kGy gamma radiation.
31038452	2	22	attach	isolated	194:201	arg1	sample					218:223	a biofilm sample	208:223	a biofilm sample collected from a radioactive thermal spring (Budapest, Hungary)	208:287	A Gram-stain-negative, aerobic, non-motile and coccus-shaped bacterium, designated strain FeSDHB5-19T, was isolated from a biofilm sample collected from a radioactive thermal spring (Budapest, Hungary), after exposure to 5 kGy gamma radiation.
31038452	13	23	from	species	1492:1498	arg1	Deinococcus					1513:1523	the genus Deinococcus	1503:1523	the genus Deinococcus	1503:1523	According to its genotypic, phenotypic and chemotaxonomic characteristics, strain FeSDHB5-19T represents a novel species in the genus Deinococcus, for which the name Deinococcusfonticola sp.
31038452	6	24	theme	optimum	858:864	arg1	growth					866:871	optimum growth	858:871	optimum growth at 0-0.5 % NaCl	858:887	Strain FeSHDB5-19T was found to grow at temperatures of 10-32 °C (optimum, 28 °C) and pH 5-10 (pH 6.5-7.5) and tolerated up to 1.5 % NaCl (w/v) with optimum growth at 0-0.5 % NaCl.
31038452	9	25	theme	whole-cell	1014:1023	arg1	sugars					1025:1030	The whole-cell sugars	1010:1030	The whole-cell sugars	1010:1030	The whole-cell sugars were glucose and low amounts of galactose.
31038452	9	25	theme	whole-cell	1014:1023	arg1	glucose					1037:1043	glucose	1037:1043	glucose	1037:1043	The whole-cell sugars were glucose and low amounts of galactose.
31038452	8	26	theme	A3β	992:994	arg1	type					983:986	The cell-wall peptidoglycan type	955:986	The cell-wall peptidoglycan type	955:986	The cell-wall peptidoglycan type was A3β l-Orn-Gly1-2.
31038452	8	26	theme	A3β	992:994	arg1	l-Orn-Gly1-2					996:1007	A3β l-Orn-Gly1-2	992:1007	A3β l-Orn-Gly1-2	992:1007	The cell-wall peptidoglycan type was A3β l-Orn-Gly1-2.
31038452	3	27	theme	gene	446:449	arg1	similarity					460:469	highest 16S rRNA gene sequence similarity	429:469	highest 16S rRNA gene sequence similarity	429:469	A polyphasic approach was used to study the taxonomic properties of strain FeSDHB5-19T, which had highest 16S rRNA gene sequence similarity to Deinococcus antarcticus G3-6-20T (96.5 %).
31038452	3	28	theme	FeSDHB5-19T	406:416	arg1	properties					385:394	the taxonomic properties	371:394	the taxonomic properties of strain FeSDHB5-19T, which had highest 16S rRNA gene sequence similarity to Deinococcus antarcticus G3-6-20T (96.5 %)	371:514	A polyphasic approach was used to study the taxonomic properties of strain FeSDHB5-19T, which had highest 16S rRNA gene sequence similarity to Deinococcus antarcticus G3-6-20T (96.5 %).
31038452	0	29	theme	fonticola	12:20	arg1	sp					22:23	Deinococcus fonticola sp	0:23	Deinococcus fonticola sp.	0:24	Deinococcus fonticola sp.
31038452	2	30	theme	non-motile	119:128	arg1	bacterium					148:156	A Gram-stain-negative, aerobic, non-motile and coccus-shaped bacterium	87:156	A Gram-stain-negative, aerobic, non-motile and coccus-shaped bacterium	87:156	A Gram-stain-negative, aerobic, non-motile and coccus-shaped bacterium, designated strain FeSDHB5-19T, was isolated from a biofilm sample collected from a radioactive thermal spring (Budapest, Hungary), after exposure to 5 kGy gamma radiation.
31038452	6	31	theme	Strain	709:714	arg1	FeSHDB5-19T					716:726	Strain FeSHDB5-19T	709:726	Strain FeSHDB5-19T	709:726	Strain FeSHDB5-19T was found to grow at temperatures of 10-32 °C (optimum, 28 °C) and pH 5-10 (pH 6.5-7.5) and tolerated up to 1.5 % NaCl (w/v) with optimum growth at 0-0.5 % NaCl.
31038452	15	32	theme	B.02639T=DSM	1627:1638	arg1	FeSDHB5-19T					1607:1617	FeSDHB5-19T	1607:1617	FeSDHB5-19T (=NCAIM B.02639T=DSM 106917T)	1607:1647	The type strain is FeSDHB5-19T (=NCAIM B.02639T=DSM 106917T).
31038452	15	32	theme	B.02639T=DSM	1627:1638	arg1	106917T					1640:1646	=NCAIM B.02639T=DSM 106917T	1620:1646	=NCAIM B.02639T=DSM 106917T	1620:1646	The type strain is FeSDHB5-19T (=NCAIM B.02639T=DSM 106917T).
31038452	5	33	theme	draft	649:653	arg1	sequence					662:669	the draft genome sequence	645:669	the draft genome sequence	645:669	The DNA G+C content of the draft genome sequence, consisting of 3.9 Mb, was 63.9 mol%.
31038452	0	34	theme	Deinococcus	0:10	arg1	sp					22:23	Deinococcus fonticola sp	0:23	Deinococcus fonticola sp.	0:24	Deinococcus fonticola sp.
31038452	9	35	theme	galactose	1064:1072	arg1	amounts					1053:1059	low amounts	1049:1059	low amounts of galactose	1049:1072	The whole-cell sugars were glucose and low amounts of galactose.
31038452	9	35	theme	galactose	1064:1072	arg1	sugars					1025:1030	The whole-cell sugars	1010:1030	The whole-cell sugars	1010:1030	The whole-cell sugars were glucose and low amounts of galactose.
31038452	9	35	theme	galactose	1064:1072	arg1	galactose					1064:1072	galactose	1064:1072	galactose	1064:1072	The whole-cell sugars were glucose and low amounts of galactose.
31038452	9	35	theme	galactose	1064:1072	arg1	glucose					1037:1043	glucose	1037:1043	glucose	1037:1043	The whole-cell sugars were glucose and low amounts of galactose.
31038452	9	36	theme	low	1049:1051	arg1	amounts					1053:1059	low amounts	1049:1059	low amounts of galactose	1049:1072	The whole-cell sugars were glucose and low amounts of galactose.
31038452	9	36	theme	low	1049:1051	arg1	galactose					1064:1072	galactose	1064:1072	galactose	1064:1072	The whole-cell sugars were glucose and low amounts of galactose.
31038452	3	37	theme	polyphasic	333:342	arg1	approach					344:351	A polyphasic approach	331:351	A polyphasic approach	331:351	A polyphasic approach was used to study the taxonomic properties of strain FeSDHB5-19T, which had highest 16S rRNA gene sequence similarity to Deinococcus antarcticus G3-6-20T (96.5 %).
31038452	6	38	theme	%	840:840	arg1	w/v					848:850	w/v	848:850	w/v	848:850	Strain FeSHDB5-19T was found to grow at temperatures of 10-32 °C (optimum, 28 °C) and pH 5-10 (pH 6.5-7.5) and tolerated up to 1.5 % NaCl (w/v) with optimum growth at 0-0.5 % NaCl.
31038452	6	38	theme	%	840:840	arg1	NaCl					842:845	1.5 % NaCl	836:845	1.5 % NaCl (w/v)	836:851	Strain FeSHDB5-19T was found to grow at temperatures of 10-32 °C (optimum, 28 °C) and pH 5-10 (pH 6.5-7.5) and tolerated up to 1.5 % NaCl (w/v) with optimum growth at 0-0.5 % NaCl.
31038452	3	39	dep	Deinococcus	474:484	arg1	antarcticus					486:496	antarcticus	486:496	antarcticus	486:496	A polyphasic approach was used to study the taxonomic properties of strain FeSDHB5-19T, which had highest 16S rRNA gene sequence similarity to Deinococcus antarcticus G3-6-20T (96.5 %).
31038452	4	40	theme	Deinococcus	580:590	arg1	species					592:598	other Deinococcus species	574:598	other Deinococcus species	574:598	The 16S rRNA gene sequence similarity to type strains of other Deinococcus species were 93.0 % or lower.
31038452	13	41	theme	genotypic	1396:1404	arg1	characteristics					1437:1451	its genotypic, phenotypic and chemotaxonomic characteristics	1392:1451	its genotypic, phenotypic and chemotaxonomic characteristics	1392:1451	According to its genotypic, phenotypic and chemotaxonomic characteristics, strain FeSDHB5-19T represents a novel species in the genus Deinococcus, for which the name Deinococcusfonticola sp.
31038452	4	42	theme	16S	521:523	arg1	%					610:610	93.0 % or lower	605:619	93.0 % or lower	605:619	The 16S rRNA gene sequence similarity to type strains of other Deinococcus species were 93.0 % or lower.
31038452	4	42	theme	16S	521:523	arg1	similarity					544:553	The 16S rRNA gene sequence similarity	517:553	The 16S rRNA gene sequence similarity to type strains of other Deinococcus species	517:598	The 16S rRNA gene sequence similarity to type strains of other Deinococcus species were 93.0 % or lower.
31038452	11	43	contain	contained	1207:1215	arg2	glycolipids					1266:1276	unidentified glycolipids	1253:1276	unidentified glycolipids	1253:1276	The polar lipid profile contained unidentified phosphoglycolipids and unidentified glycolipids.
31038452	11	43	contain	contained	1207:1215	arg1	profile					1199:1205	The polar lipid profile	1183:1205	The polar lipid profile	1183:1205	The polar lipid profile contained unidentified phosphoglycolipids and unidentified glycolipids.
31038452	11	43	contain	contained	1207:1215	arg2	phosphoglycolipids					1230:1247	unidentified phosphoglycolipids	1217:1247	unidentified phosphoglycolipids	1217:1247	The polar lipid profile contained unidentified phosphoglycolipids and unidentified glycolipids.
31038452	10	44	theme	respiratory	1128:1138	arg1	quinone					1140:1146	the predominant respiratory quinone	1112:1146	the predominant respiratory quinone	1112:1146	Strain FeSDHB5-19T possessed MK-8 as the predominant respiratory quinone, typical of the genus Deinococcus.
31038452	10	44	theme	respiratory	1128:1138	arg1	MK-8					1104:1107	MK-8	1104:1107	MK-8	1104:1107	Strain FeSDHB5-19T possessed MK-8 as the predominant respiratory quinone, typical of the genus Deinococcus.
31038452	3	45	theme	highest	429:435	arg1	similarity					460:469	highest 16S rRNA gene sequence similarity	429:469	highest 16S rRNA gene sequence similarity	429:469	A polyphasic approach was used to study the taxonomic properties of strain FeSDHB5-19T, which had highest 16S rRNA gene sequence similarity to Deinococcus antarcticus G3-6-20T (96.5 %).
31038452	13	46	theme	novel	1486:1490	arg1	species					1492:1498	a novel species	1484:1498	a novel species	1484:1498	According to its genotypic, phenotypic and chemotaxonomic characteristics, strain FeSDHB5-19T represents a novel species in the genus Deinococcus, for which the name Deinococcusfonticola sp.
31038452	6	47	theme	1.5 	836:839	arg1	%					840:840	%	840:840	%	840:840	Strain FeSHDB5-19T was found to grow at temperatures of 10-32 °C (optimum, 28 °C) and pH 5-10 (pH 6.5-7.5) and tolerated up to 1.5 % NaCl (w/v) with optimum growth at 0-0.5 % NaCl.
31038452	3	48	used	used	357:360	arg2	approach					344:351	A polyphasic approach	331:351	A polyphasic approach	331:351	A polyphasic approach was used to study the taxonomic properties of strain FeSDHB5-19T, which had highest 16S rRNA gene sequence similarity to Deinococcus antarcticus G3-6-20T (96.5 %).
31038452	2	49	theme	strain	170:175	arg1	FeSDHB5-19T					177:187	strain FeSDHB5-19T	170:187	strain FeSDHB5-19T	170:187	A Gram-stain-negative, aerobic, non-motile and coccus-shaped bacterium, designated strain FeSDHB5-19T, was isolated from a biofilm sample collected from a radioactive thermal spring (Budapest, Hungary), after exposure to 5 kGy gamma radiation.
31038452	10	50	theme	predominant	1116:1126	arg1	quinone					1140:1146	the predominant respiratory quinone	1112:1146	the predominant respiratory quinone	1112:1146	Strain FeSDHB5-19T possessed MK-8 as the predominant respiratory quinone, typical of the genus Deinococcus.
31038452	10	50	theme	predominant	1116:1126	arg1	MK-8					1104:1107	MK-8	1104:1107	MK-8	1104:1107	Strain FeSDHB5-19T possessed MK-8 as the predominant respiratory quinone, typical of the genus Deinococcus.
31038452	15	51	theme	=NCAIM	1620:1625	arg1	FeSDHB5-19T					1607:1617	FeSDHB5-19T	1607:1617	FeSDHB5-19T (=NCAIM B.02639T=DSM 106917T)	1607:1647	The type strain is FeSDHB5-19T (=NCAIM B.02639T=DSM 106917T).
31038452	15	51	theme	=NCAIM	1620:1625	arg1	106917T					1640:1646	=NCAIM B.02639T=DSM 106917T	1620:1646	=NCAIM B.02639T=DSM 106917T	1620:1646	The type strain is FeSDHB5-19T (=NCAIM B.02639T=DSM 106917T).
31038452	3	52	theme	strain	399:404	arg1	FeSDHB5-19T					406:416	strain FeSDHB5-19T	399:416	strain FeSDHB5-19T	399:416	A polyphasic approach was used to study the taxonomic properties of strain FeSDHB5-19T, which had highest 16S rRNA gene sequence similarity to Deinococcus antarcticus G3-6-20T (96.5 %).
31038452	6	53	dep	optimum	775:781	arg1	28 °C					784:788	28 °C	784:788	28 °C	784:788	Strain FeSHDB5-19T was found to grow at temperatures of 10-32 °C (optimum, 28 °C) and pH 5-10 (pH 6.5-7.5) and tolerated up to 1.5 % NaCl (w/v) with optimum growth at 0-0.5 % NaCl.
31038452	3	54	theme	taxonomic	375:383	arg1	properties					385:394	the taxonomic properties	371:394	the taxonomic properties of strain FeSDHB5-19T, which had highest 16S rRNA gene sequence similarity to Deinococcus antarcticus G3-6-20T (96.5 %)	371:514	A polyphasic approach was used to study the taxonomic properties of strain FeSDHB5-19T, which had highest 16S rRNA gene sequence similarity to Deinococcus antarcticus G3-6-20T (96.5 %).
31038452	10	55	theme	Strain	1075:1080	arg1	FeSDHB5-19T					1082:1092	Strain FeSDHB5-19T	1075:1092	Strain FeSDHB5-19T	1075:1092	Strain FeSDHB5-19T possessed MK-8 as the predominant respiratory quinone, typical of the genus Deinococcus.
31038452	10	56	theme	genus	1164:1168	arg1	Deinococcus					1170:1180	the genus Deinococcus	1160:1180	the genus Deinococcus	1160:1180	Strain FeSDHB5-19T possessed MK-8 as the predominant respiratory quinone, typical of the genus Deinococcus.
31038452	4	57	theme	species	592:598	arg1	strains					563:569	type strains	558:569	type strains of other Deinococcus species	558:598	The 16S rRNA gene sequence similarity to type strains of other Deinococcus species were 93.0 % or lower.
31038452	8	58	theme	peptidoglycan	969:981	arg1	type					983:986	The cell-wall peptidoglycan type	955:986	The cell-wall peptidoglycan type	955:986	The cell-wall peptidoglycan type was A3β l-Orn-Gly1-2.
31038452	8	58	theme	peptidoglycan	969:981	arg1	l-Orn-Gly1-2					996:1007	A3β l-Orn-Gly1-2	992:1007	A3β l-Orn-Gly1-2	992:1007	The cell-wall peptidoglycan type was A3β l-Orn-Gly1-2.
31038452	13	59	theme	strain	1454:1459	arg1	FeSDHB5-19T					1461:1471	strain FeSDHB5-19T	1454:1471	strain FeSDHB5-19T	1454:1471	According to its genotypic, phenotypic and chemotaxonomic characteristics, strain FeSDHB5-19T represents a novel species in the genus Deinococcus, for which the name Deinococcusfonticola sp.
31038452	3	60	contain	had	425:427	arg1	FeSDHB5-19T					406:416	strain FeSDHB5-19T	399:416	strain FeSDHB5-19T	399:416	A polyphasic approach was used to study the taxonomic properties of strain FeSDHB5-19T, which had highest 16S rRNA gene sequence similarity to Deinococcus antarcticus G3-6-20T (96.5 %).
31038452	3	60	contain	had	425:427	arg2	similarity					460:469	highest 16S rRNA gene sequence similarity	429:469	highest 16S rRNA gene sequence similarity	429:469	A polyphasic approach was used to study the taxonomic properties of strain FeSDHB5-19T, which had highest 16S rRNA gene sequence similarity to Deinococcus antarcticus G3-6-20T (96.5 %).
31038452	2	61	theme	biofilm	210:216	arg1	sample					218:223	a biofilm sample	208:223	a biofilm sample collected from a radioactive thermal spring (Budapest, Hungary)	208:287	A Gram-stain-negative, aerobic, non-motile and coccus-shaped bacterium, designated strain FeSDHB5-19T, was isolated from a biofilm sample collected from a radioactive thermal spring (Budapest, Hungary), after exposure to 5 kGy gamma radiation.
31038452	2	62	theme	kGy	310:312	arg1	radiation					320:328	5 kGy gamma radiation	308:328	5 kGy gamma radiation	308:328	A Gram-stain-negative, aerobic, non-motile and coccus-shaped bacterium, designated strain FeSDHB5-19T, was isolated from a biofilm sample collected from a radioactive thermal spring (Budapest, Hungary), after exposure to 5 kGy gamma radiation.
31038452	5	63	theme	genome	655:660	arg1	sequence					662:669	the draft genome sequence	645:669	the draft genome sequence	645:669	The DNA G+C content of the draft genome sequence, consisting of 3.9 Mb, was 63.9 mol%.
31038452	7	64	theme	predominant	894:904	arg1	acids					912:916	The predominant fatty acids	890:916	The predominant fatty acids (>10 %)	890:924	The predominant fatty acids (>10 %) were C16 : 0 and C16 : 1ω7c.
31038452	7	64	theme	predominant	894:904	arg1	%					923:923	>10 %	919:923	>10 %	919:923	The predominant fatty acids (>10 %) were C16 : 0 and C16 : 1ω7c.
31038452	7	64	theme	predominant	894:904	arg1	C16 					931:934	C16 	931:934	C16 	931:934	The predominant fatty acids (>10 %) were C16 : 0 and C16 : 1ω7c.
31038452	6	65	theme	10-32 °C	765:772	arg1	pH					795:796	pH 5-10	795:801	pH 5-10 (pH 6.5-7.5)	795:814	Strain FeSHDB5-19T was found to grow at temperatures of 10-32 °C (optimum, 28 °C) and pH 5-10 (pH 6.5-7.5) and tolerated up to 1.5 % NaCl (w/v) with optimum growth at 0-0.5 % NaCl.
31038452	6	65	theme	10-32 °C	765:772	arg1	pH					804:805	pH 6.5-7.5	804:813	pH 6.5-7.5	804:813	Strain FeSHDB5-19T was found to grow at temperatures of 10-32 °C (optimum, 28 °C) and pH 5-10 (pH 6.5-7.5) and tolerated up to 1.5 % NaCl (w/v) with optimum growth at 0-0.5 % NaCl.
31038452	6	65	theme	10-32 °C	765:772	arg1	temperatures					749:760	temperatures	749:760	temperatures of 10-32 °C (optimum, 28 °C)	749:789	Strain FeSHDB5-19T was found to grow at temperatures of 10-32 °C (optimum, 28 °C) and pH 5-10 (pH 6.5-7.5) and tolerated up to 1.5 % NaCl (w/v) with optimum growth at 0-0.5 % NaCl.
31038452	3	66	theme	sequence	451:458	arg1	similarity					460:469	highest 16S rRNA gene sequence similarity	429:469	highest 16S rRNA gene sequence similarity	429:469	A polyphasic approach was used to study the taxonomic properties of strain FeSDHB5-19T, which had highest 16S rRNA gene sequence similarity to Deinococcus antarcticus G3-6-20T (96.5 %).
31038452	5	67	theme	DNA	626:628	arg1	%					706:706	63.9 mol%	698:706	63.9 mol%	698:706	The DNA G+C content of the draft genome sequence, consisting of 3.9 Mb, was 63.9 mol%.
31038452	5	67	theme	DNA	626:628	arg1	G+C content					630:640	The DNA G+C content	622:640	The DNA G+C content of the draft genome sequence, consisting of 3.9 Mb,	622:692	The DNA G+C content of the draft genome sequence, consisting of 3.9 Mb, was 63.9 mol%.
31038452	6	68	from	 NaCl	883:887	arg1	growth					866:871	optimum growth	858:871	optimum growth at 0-0.5 % NaCl	858:887	Strain FeSHDB5-19T was found to grow at temperatures of 10-32 °C (optimum, 28 °C) and pH 5-10 (pH 6.5-7.5) and tolerated up to 1.5 % NaCl (w/v) with optimum growth at 0-0.5 % NaCl.
31038452	2	69	theme	thermal	254:260	arg1	spring					262:267	a radioactive thermal spring	240:267	a radioactive thermal spring (Budapest, Hungary)	240:287	A Gram-stain-negative, aerobic, non-motile and coccus-shaped bacterium, designated strain FeSDHB5-19T, was isolated from a biofilm sample collected from a radioactive thermal spring (Budapest, Hungary), after exposure to 5 kGy gamma radiation.
31038452	2	69	theme	thermal	254:260	arg1	Hungary					280:286	Hungary	280:286	Hungary	280:286	A Gram-stain-negative, aerobic, non-motile and coccus-shaped bacterium, designated strain FeSDHB5-19T, was isolated from a biofilm sample collected from a radioactive thermal spring (Budapest, Hungary), after exposure to 5 kGy gamma radiation.
31038452	10	70	theme	typical	1149:1155	arg1	quinone					1140:1146	the predominant respiratory quinone	1112:1146	the predominant respiratory quinone	1112:1146	Strain FeSDHB5-19T possessed MK-8 as the predominant respiratory quinone, typical of the genus Deinococcus.
31038452	10	70	theme	typical	1149:1155	arg1	MK-8					1104:1107	MK-8	1104:1107	MK-8	1104:1107	Strain FeSDHB5-19T possessed MK-8 as the predominant respiratory quinone, typical of the genus Deinococcus.
31038452	3	71	theme	16S	437:439	arg1	similarity					460:469	highest 16S rRNA gene sequence similarity	429:469	highest 16S rRNA gene sequence similarity	429:469	A polyphasic approach was used to study the taxonomic properties of strain FeSDHB5-19T, which had highest 16S rRNA gene sequence similarity to Deinococcus antarcticus G3-6-20T (96.5 %).
31038452	15	72	theme	type	1592:1595	arg1	FeSDHB5-19T					1607:1617	FeSDHB5-19T	1607:1617	FeSDHB5-19T (=NCAIM B.02639T=DSM 106917T)	1607:1647	The type strain is FeSDHB5-19T (=NCAIM B.02639T=DSM 106917T).
31038452	15	72	theme	type	1592:1595	arg1	strain					1597:1602	The type strain	1588:1602	The type strain	1588:1602	The type strain is FeSDHB5-19T (=NCAIM B.02639T=DSM 106917T).
31038452	4	73	theme	type	558:561	arg1	strains					563:569	type strains	558:569	type strains of other Deinococcus species	558:598	The 16S rRNA gene sequence similarity to type strains of other Deinococcus species were 93.0 % or lower.
31038452	2	74	theme	radioactive	242:252	arg1	spring					262:267	a radioactive thermal spring	240:267	a radioactive thermal spring (Budapest, Hungary)	240:287	A Gram-stain-negative, aerobic, non-motile and coccus-shaped bacterium, designated strain FeSDHB5-19T, was isolated from a biofilm sample collected from a radioactive thermal spring (Budapest, Hungary), after exposure to 5 kGy gamma radiation.
31038452	2	74	theme	radioactive	242:252	arg1	Hungary					280:286	Hungary	280:286	Hungary	280:286	A Gram-stain-negative, aerobic, non-motile and coccus-shaped bacterium, designated strain FeSDHB5-19T, was isolated from a biofilm sample collected from a radioactive thermal spring (Budapest, Hungary), after exposure to 5 kGy gamma radiation.
31038452	11	75	theme	polar	1187:1191	arg1	profile					1199:1205	The polar lipid profile	1183:1205	The polar lipid profile	1183:1205	The polar lipid profile contained unidentified phosphoglycolipids and unidentified glycolipids.
31038452	3	76	theme	rRNA	441:444	arg1	similarity					460:469	highest 16S rRNA gene sequence similarity	429:469	highest 16S rRNA gene sequence similarity	429:469	A polyphasic approach was used to study the taxonomic properties of strain FeSDHB5-19T, which had highest 16S rRNA gene sequence similarity to Deinococcus antarcticus G3-6-20T (96.5 %).
31038452	6	77	theme	0-0.5 	876:881	arg1	%					882:882	%	882:882	%	882:882	Strain FeSHDB5-19T was found to grow at temperatures of 10-32 °C (optimum, 28 °C) and pH 5-10 (pH 6.5-7.5) and tolerated up to 1.5 % NaCl (w/v) with optimum growth at 0-0.5 % NaCl.
31038452	6	78	dep	NaCl	842:845	arg1	up					830:831	up	830:831	up	830:831	Strain FeSHDB5-19T was found to grow at temperatures of 10-32 °C (optimum, 28 °C) and pH 5-10 (pH 6.5-7.5) and tolerated up to 1.5 % NaCl (w/v) with optimum growth at 0-0.5 % NaCl.
31038452	3	79	theme	Deinococcus	474:484	arg1	G3-6-20T					498:505	Deinococcus antarcticus G3-6-20T	474:505	Deinococcus antarcticus G3-6-20T (96.5 %)	474:514	A polyphasic approach was used to study the taxonomic properties of strain FeSDHB5-19T, which had highest 16S rRNA gene sequence similarity to Deinococcus antarcticus G3-6-20T (96.5 %).
31038452	3	79	theme	Deinococcus	474:484	arg1	%					513:513	96.5 %	508:513	96.5 %	508:513	A polyphasic approach was used to study the taxonomic properties of strain FeSDHB5-19T, which had highest 16S rRNA gene sequence similarity to Deinococcus antarcticus G3-6-20T (96.5 %).
31038452	11	80	theme	unidentified	1217:1228	arg1	phosphoglycolipids					1230:1247	unidentified phosphoglycolipids	1217:1247	unidentified phosphoglycolipids	1217:1247	The polar lipid profile contained unidentified phosphoglycolipids and unidentified glycolipids.
31038452	10	81	contain	possessed	1094:1102	arg2	quinone					1140:1146	the predominant respiratory quinone	1112:1146	the predominant respiratory quinone	1112:1146	Strain FeSDHB5-19T possessed MK-8 as the predominant respiratory quinone, typical of the genus Deinococcus.
31038452	10	81	contain	possessed	1094:1102	arg2	MK-8					1104:1107	MK-8	1104:1107	MK-8	1104:1107	Strain FeSDHB5-19T possessed MK-8 as the predominant respiratory quinone, typical of the genus Deinococcus.
31038452	10	81	contain	possessed	1094:1102	arg1	FeSDHB5-19T					1082:1092	Strain FeSDHB5-19T	1075:1092	Strain FeSDHB5-19T	1075:1092	Strain FeSDHB5-19T possessed MK-8 as the predominant respiratory quinone, typical of the genus Deinococcus.
31038452	7	82	theme	fatty	906:910	arg1	acids					912:916	The predominant fatty acids	890:916	The predominant fatty acids (>10 %)	890:924	The predominant fatty acids (>10 %) were C16 : 0 and C16 : 1ω7c.
31038452	7	82	theme	fatty	906:910	arg1	%					923:923	>10 %	919:923	>10 %	919:923	The predominant fatty acids (>10 %) were C16 : 0 and C16 : 1ω7c.
31038452	7	82	theme	fatty	906:910	arg1	C16 					931:934	C16 	931:934	C16 	931:934	The predominant fatty acids (>10 %) were C16 : 0 and C16 : 1ω7c.
31038452	12	83	theme	UV	1349:1350	arg1	J m-2					1361:1365	D10~800 J m-2	1353:1365	D10~800 J m-2	1353:1365	The isolate was found to be highly resistant to gamma (D10<8 kGy) and UV (D10~800 J m-2) radiation.
31038452	12	83	theme	UV	1349:1350	arg1	radiation					1368:1376	gamma (D10<8 kGy) and UV (D10~800 J m-2) radiation	1327:1376	radiation	1368:1376	The isolate was found to be highly resistant to gamma (D10<8 kGy) and UV (D10~800 J m-2) radiation.
31038452	6	84	theme	%	882:882	arg1	 NaCl					883:887	0-0.5 % NaCl	876:887	0-0.5 % NaCl	876:887	Strain FeSHDB5-19T was found to grow at temperatures of 10-32 °C (optimum, 28 °C) and pH 5-10 (pH 6.5-7.5) and tolerated up to 1.5 % NaCl (w/v) with optimum growth at 0-0.5 % NaCl.
31726738	4	0	theme	potential	944:952	arg1	functions					954:962	their potential functions	938:962	their potential functions	938:962	To investigate the relationships of IN, colitis, and gut microbiomes, a dextran sulfate sodium (DSS)-induced mice colitis model was tested to explore the protective effects of IN on macroscopic colitis symptoms, the histopathological structure, inflammation cytokines, and gut microbiota, and their potential functions.
31726738	3	1	theme	flora	470:474	arg1	alterations					444:454	alterations	444:454	alterations of intestinal flora related to IBD	444:489	Regarding the significance of alterations of intestinal flora related to IBD and the poor water solubility of the blue IN powder, we predicted that the protective action of IN on colitis may occur through modifying gut microbiota.
31726738	10	2	theme	neolacto	2199:2206	arg1	series					2208:2213	neolacto series	2199:2213	neolacto series	2199:2213	The inferred metagenomes from 16S data using PICRUSt demonstrated that decreased metabolic genes, such as through biosynthesis of siderophore group nonribosomal peptides, non-homologous end-joining, and glycosphingolipid biosynthesis of lacto and neolacto series, may maintain microbiota homeostasis during inflammation from IN treatment in DSS-induced colitis.
31726738	6	3	theme	indirubin	1100:1108	arg1	compositions					1073:1084	the main chemical compositions	1055:1084	the main chemical compositions of indigo and indirubin in IN	1055:1114	Firstly, because it was a mixture, the main chemical compositions of indigo and indirubin in IN were detected by ultra-performance liquid chromatography (UPLC).
31726738	6	4	theme	main	1059:1062	arg1	compositions					1073:1084	the main chemical compositions	1055:1084	the main chemical compositions of indigo and indirubin in IN	1055:1114	Firstly, because it was a mixture, the main chemical compositions of indigo and indirubin in IN were detected by ultra-performance liquid chromatography (UPLC).
31726738	10	5	theme	16S	1982:1984	arg1	data					1986:1989	16S data	1982:1989	16S data using PICRUSt	1982:2003	The inferred metagenomes from 16S data using PICRUSt demonstrated that decreased metabolic genes, such as through biosynthesis of siderophore group nonribosomal peptides, non-homologous end-joining, and glycosphingolipid biosynthesis of lacto and neolacto series, may maintain microbiota homeostasis during inflammation from IN treatment in DSS-induced colitis.
31726738	3	6	from	action	577:582	arg1	colitis					593:599	colitis	593:599	colitis	593:599	Regarding the significance of alterations of intestinal flora related to IBD and the poor water solubility of the blue IN powder, we predicted that the protective action of IN on colitis may occur through modifying gut microbiota.
31726738	6	7	theme	liquid	1151:1156	arg1	chromatography					1158:1171	ultra-performance liquid chromatography	1133:1171	ultra-performance liquid chromatography (UPLC)	1133:1178	Firstly, because it was a mixture, the main chemical compositions of indigo and indirubin in IN were detected by ultra-performance liquid chromatography (UPLC).
31726738	6	7	theme	liquid	1151:1156	arg1	UPLC					1174:1177	UPLC	1174:1177	UPLC	1174:1177	Firstly, because it was a mixture, the main chemical compositions of indigo and indirubin in IN were detected by ultra-performance liquid chromatography (UPLC).
31726738	3	8	theme	poor	499:502	arg1	solubility					510:519	the poor water solubility	495:519	the poor water solubility of the blue IN powder	495:541	Regarding the significance of alterations of intestinal flora related to IBD and the poor water solubility of the blue IN powder, we predicted that the protective action of IN on colitis may occur through modifying gut microbiota.
31726738	8	9	theme	16S	1581:1583	arg1	sequences					1590:1598	16S rDNA sequences	1581:1598	16S rDNA sequences analysis with the Illumina MiSeq platform	1581:1640	The results of 16S rDNA sequences analysis with the Illumina MiSeq platform showed that IN could modulate the balance of gut microbiota, especially by down-regulating the relative quantity of Turicibacter and up-regulating the relative quantity of Peptococcus.
31726738	4	10	theme	-induced	745:752	arg1	model					767:771	a dextran sulfate sodium (DSS)-induced mice colitis model	715:771	a dextran sulfate sodium (DSS)-induced mice colitis model	715:771	To investigate the relationships of IN, colitis, and gut microbiomes, a dextran sulfate sodium (DSS)-induced mice colitis model was tested to explore the protective effects of IN on macroscopic colitis symptoms, the histopathological structure, inflammation cytokines, and gut microbiota, and their potential functions.
31726738	7	11	theme	enzyme-linked	1266:1278	arg1	ELISA					1301:1305	ELISA	1301:1305	ELISA	1301:1305	The clinical activity score (CAS), hematoxylin and eosin (H&E) staining results, and enzyme-linked immunosorbent assay (ELISA) results in this study showed that IN greatly improved the health conditions of the tested colitis mice, ameliorated the histopathological structure of the colon tissue, down-regulated pro-inflammatory cytokines, and up-regulated anti-inflammatory cytokines.
31726738	7	11	theme	enzyme-linked	1266:1278	arg1	assay					1294:1298	enzyme-linked immunosorbent assay	1266:1298	enzyme-linked immunosorbent assay (ELISA)	1266:1306	The clinical activity score (CAS), hematoxylin and eosin (H&E) staining results, and enzyme-linked immunosorbent assay (ELISA) results in this study showed that IN greatly improved the health conditions of the tested colitis mice, ameliorated the histopathological structure of the colon tissue, down-regulated pro-inflammatory cytokines, and up-regulated anti-inflammatory cytokines.
31726738	10	12	theme	decreased	2023:2031	arg1	biosynthesis					2173:2184	glycosphingolipid biosynthesis	2155:2184	glycosphingolipid biosynthesis of lacto and neolacto series	2155:2213	The inferred metagenomes from 16S data using PICRUSt demonstrated that decreased metabolic genes, such as through biosynthesis of siderophore group nonribosomal peptides, non-homologous end-joining, and glycosphingolipid biosynthesis of lacto and neolacto series, may maintain microbiota homeostasis during inflammation from IN treatment in DSS-induced colitis.
31726738	10	12	theme	decreased	2023:2031	arg1	biosynthesis					2066:2077	biosynthesis	2066:2077	biosynthesis of siderophore group nonribosomal peptides	2066:2120	The inferred metagenomes from 16S data using PICRUSt demonstrated that decreased metabolic genes, such as through biosynthesis of siderophore group nonribosomal peptides, non-homologous end-joining, and glycosphingolipid biosynthesis of lacto and neolacto series, may maintain microbiota homeostasis during inflammation from IN treatment in DSS-induced colitis.
31726738	10	12	theme	decreased	2023:2031	arg1	genes					2043:2047	decreased metabolic genes	2023:2047	decreased metabolic genes	2023:2047	The inferred metagenomes from 16S data using PICRUSt demonstrated that decreased metabolic genes, such as through biosynthesis of siderophore group nonribosomal peptides, non-homologous end-joining, and glycosphingolipid biosynthesis of lacto and neolacto series, may maintain microbiota homeostasis during inflammation from IN treatment in DSS-induced colitis.
31726738	10	12	theme	decreased	2023:2031	arg1	end-joining					2138:2148	non-homologous end-joining	2123:2148	non-homologous end-joining	2123:2148	The inferred metagenomes from 16S data using PICRUSt demonstrated that decreased metabolic genes, such as through biosynthesis of siderophore group nonribosomal peptides, non-homologous end-joining, and glycosphingolipid biosynthesis of lacto and neolacto series, may maintain microbiota homeostasis during inflammation from IN treatment in DSS-induced colitis.
31726738	8	13	theme	Turicibacter	1758:1769	arg1	quantity					1746:1753	the relative quantity	1733:1753	the relative quantity of Turicibacter	1733:1769	The results of 16S rDNA sequences analysis with the Illumina MiSeq platform showed that IN could modulate the balance of gut microbiota, especially by down-regulating the relative quantity of Turicibacter and up-regulating the relative quantity of Peptococcus.
31726738	3	14	from	significance	428:439	arg1	powder					536:541	powder	536:541	powder	536:541	Regarding the significance of alterations of intestinal flora related to IBD and the poor water solubility of the blue IN powder, we predicted that the protective action of IN on colitis may occur through modifying gut microbiota.
31726738	8	15	theme	sequences	1590:1598	arg1	results					1570:1576	The results	1566:1576	The results of 16S rDNA sequences analysis with the Illumina MiSeq platform	1566:1640	The results of 16S rDNA sequences analysis with the Illumina MiSeq platform showed that IN could modulate the balance of gut microbiota, especially by down-regulating the relative quantity of Turicibacter and up-regulating the relative quantity of Peptococcus.
31726738	4	16	theme	colitis	759:765	arg1	model					767:771	a dextran sulfate sodium (DSS)-induced mice colitis model	715:771	a dextran sulfate sodium (DSS)-induced mice colitis model	715:771	To investigate the relationships of IN, colitis, and gut microbiomes, a dextran sulfate sodium (DSS)-induced mice colitis model was tested to explore the protective effects of IN on macroscopic colitis symptoms, the histopathological structure, inflammation cytokines, and gut microbiota, and their potential functions.
31726738	7	17	from	results	1308:1314	arg1	study					1324:1328	this study	1319:1328	this study	1319:1328	The clinical activity score (CAS), hematoxylin and eosin (H&E) staining results, and enzyme-linked immunosorbent assay (ELISA) results in this study showed that IN greatly improved the health conditions of the tested colitis mice, ameliorated the histopathological structure of the colon tissue, down-regulated pro-inflammatory cytokines, and up-regulated anti-inflammatory cytokines.
31726738	4	18	theme	IN	821:822	arg1	effects					810:816	the protective effects	795:816	the protective effects of IN on macroscopic colitis symptoms, the histopathological structure, inflammation cytokines, and gut microbiota, and their potential functions	795:962	To investigate the relationships of IN, colitis, and gut microbiomes, a dextran sulfate sodium (DSS)-induced mice colitis model was tested to explore the protective effects of IN on macroscopic colitis symptoms, the histopathological structure, inflammation cytokines, and gut microbiota, and their potential functions.
31726738	7	19	theme	staining	1244:1251	arg1	results					1253:1259	hematoxylin and eosin (H&E) staining results	1216:1259	hematoxylin and eosin (H&E) staining results	1216:1259	The clinical activity score (CAS), hematoxylin and eosin (H&E) staining results, and enzyme-linked immunosorbent assay (ELISA) results in this study showed that IN greatly improved the health conditions of the tested colitis mice, ameliorated the histopathological structure of the colon tissue, down-regulated pro-inflammatory cytokines, and up-regulated anti-inflammatory cytokines.
31726738	8	20	theme	relative	1793:1800	arg1	quantity					1802:1809	the relative quantity	1789:1809	the relative quantity of Peptococcus	1789:1824	The results of 16S rDNA sequences analysis with the Illumina MiSeq platform showed that IN could modulate the balance of gut microbiota, especially by down-regulating the relative quantity of Turicibacter and up-regulating the relative quantity of Peptococcus.
31726738	8	21	theme	Illumina	1618:1625	arg1	platform					1633:1640	the Illumina MiSeq platform	1614:1640	the Illumina MiSeq platform	1614:1640	The results of 16S rDNA sequences analysis with the Illumina MiSeq platform showed that IN could modulate the balance of gut microbiota, especially by down-regulating the relative quantity of Turicibacter and up-regulating the relative quantity of Peptococcus.
31726738	4	22	theme	macroscopic	827:837	arg1	symptoms					847:854	macroscopic colitis symptoms	827:854	macroscopic colitis symptoms	827:854	To investigate the relationships of IN, colitis, and gut microbiomes, a dextran sulfate sodium (DSS)-induced mice colitis model was tested to explore the protective effects of IN on macroscopic colitis symptoms, the histopathological structure, inflammation cytokines, and gut microbiota, and their potential functions.
31726738	7	23	theme	tissue	1469:1474	arg1	structure					1446:1454	the histopathological structure	1424:1454	the histopathological structure of the colon tissue	1424:1474	The clinical activity score (CAS), hematoxylin and eosin (H&E) staining results, and enzyme-linked immunosorbent assay (ELISA) results in this study showed that IN greatly improved the health conditions of the tested colitis mice, ameliorated the histopathological structure of the colon tissue, down-regulated pro-inflammatory cytokines, and up-regulated anti-inflammatory cytokines.
31726738	7	24	theme	eosin	1232:1236	arg1	results					1253:1259	hematoxylin and eosin (H&E) staining results	1216:1259	hematoxylin and eosin (H&E) staining results	1216:1259	The clinical activity score (CAS), hematoxylin and eosin (H&E) staining results, and enzyme-linked immunosorbent assay (ELISA) results in this study showed that IN greatly improved the health conditions of the tested colitis mice, ameliorated the histopathological structure of the colon tissue, down-regulated pro-inflammatory cytokines, and up-regulated anti-inflammatory cytokines.
31726738	9	25	theme	IN	1853:1854	arg1	related					1871:1877	related	1871:1877	related	1871:1877	The therapeutic effect of IN may be closely related to the anaerobic gram-positive bacteria of Turicibacter and Peptococcus.
31726738	9	25	theme	IN	1853:1854	arg1	effect					1843:1848	The therapeutic effect	1827:1848	The therapeutic effect of IN	1827:1854	The therapeutic effect of IN may be closely related to the anaerobic gram-positive bacteria of Turicibacter and Peptococcus.
31726738	4	26	from	effects	810:816	arg1	structure					879:887	the histopathological structure	857:887	the histopathological structure	857:887	To investigate the relationships of IN, colitis, and gut microbiomes, a dextran sulfate sodium (DSS)-induced mice colitis model was tested to explore the protective effects of IN on macroscopic colitis symptoms, the histopathological structure, inflammation cytokines, and gut microbiota, and their potential functions.
31726738	4	26	from	effects	810:816	arg1	cytokines					903:911	inflammation cytokines	890:911	inflammation cytokines	890:911	To investigate the relationships of IN, colitis, and gut microbiomes, a dextran sulfate sodium (DSS)-induced mice colitis model was tested to explore the protective effects of IN on macroscopic colitis symptoms, the histopathological structure, inflammation cytokines, and gut microbiota, and their potential functions.
31726738	4	26	from	effects	810:816	arg1	symptoms					847:854	macroscopic colitis symptoms	827:854	macroscopic colitis symptoms	827:854	To investigate the relationships of IN, colitis, and gut microbiomes, a dextran sulfate sodium (DSS)-induced mice colitis model was tested to explore the protective effects of IN on macroscopic colitis symptoms, the histopathological structure, inflammation cytokines, and gut microbiota, and their potential functions.
31726738	4	26	from	effects	810:816	arg1	microbiota					922:931	gut microbiota	918:931	gut microbiota	918:931	To investigate the relationships of IN, colitis, and gut microbiomes, a dextran sulfate sodium (DSS)-induced mice colitis model was tested to explore the protective effects of IN on macroscopic colitis symptoms, the histopathological structure, inflammation cytokines, and gut microbiota, and their potential functions.
31726738	4	26	from	effects	810:816	arg1	functions					954:962	their potential functions	938:962	their potential functions	938:962	To investigate the relationships of IN, colitis, and gut microbiomes, a dextran sulfate sodium (DSS)-induced mice colitis model was tested to explore the protective effects of IN on macroscopic colitis symptoms, the histopathological structure, inflammation cytokines, and gut microbiota, and their potential functions.
31726738	6	27	theme	indigo	1089:1094	arg1	compositions					1073:1084	the main chemical compositions	1055:1084	the main chemical compositions of indigo and indirubin in IN	1055:1114	Firstly, because it was a mixture, the main chemical compositions of indigo and indirubin in IN were detected by ultra-performance liquid chromatography (UPLC).
31726738	7	28	theme	hematoxylin	1216:1226	arg1	results					1253:1259	hematoxylin and eosin (H&E) staining results	1216:1259	hematoxylin and eosin (H&E) staining results	1216:1259	The clinical activity score (CAS), hematoxylin and eosin (H&E) staining results, and enzyme-linked immunosorbent assay (ELISA) results in this study showed that IN greatly improved the health conditions of the tested colitis mice, ameliorated the histopathological structure of the colon tissue, down-regulated pro-inflammatory cytokines, and up-regulated anti-inflammatory cytokines.
31726738	3	29	from	solubility	510:519	arg1	powder					536:541	powder	536:541	powder	536:541	Regarding the significance of alterations of intestinal flora related to IBD and the poor water solubility of the blue IN powder, we predicted that the protective action of IN on colitis may occur through modifying gut microbiota.
31726738	7	30	theme	assay	1294:1298	arg1	results					1308:1314	The clinical activity score (CAS), hematoxylin and eosin (H&E) staining results, and enzyme-linked immunosorbent assay (ELISA) results	1181:1314	The clinical activity score (CAS), hematoxylin and eosin (H&E) staining results, and enzyme-linked immunosorbent assay (ELISA) results in this study	1181:1328	The clinical activity score (CAS), hematoxylin and eosin (H&E) staining results, and enzyme-linked immunosorbent assay (ELISA) results in this study showed that IN greatly improved the health conditions of the tested colitis mice, ameliorated the histopathological structure of the colon tissue, down-regulated pro-inflammatory cytokines, and up-regulated anti-inflammatory cytokines.
31726738	10	31	theme	peptides	2113:2120	arg1	biosynthesis					2173:2184	glycosphingolipid biosynthesis	2155:2184	glycosphingolipid biosynthesis of lacto and neolacto series	2155:2213	The inferred metagenomes from 16S data using PICRUSt demonstrated that decreased metabolic genes, such as through biosynthesis of siderophore group nonribosomal peptides, non-homologous end-joining, and glycosphingolipid biosynthesis of lacto and neolacto series, may maintain microbiota homeostasis during inflammation from IN treatment in DSS-induced colitis.
31726738	10	31	theme	peptides	2113:2120	arg1	biosynthesis					2066:2077	biosynthesis	2066:2077	biosynthesis of siderophore group nonribosomal peptides	2066:2120	The inferred metagenomes from 16S data using PICRUSt demonstrated that decreased metabolic genes, such as through biosynthesis of siderophore group nonribosomal peptides, non-homologous end-joining, and glycosphingolipid biosynthesis of lacto and neolacto series, may maintain microbiota homeostasis during inflammation from IN treatment in DSS-induced colitis.
31726738	10	31	theme	peptides	2113:2120	arg1	end-joining					2138:2148	non-homologous end-joining	2123:2148	non-homologous end-joining	2123:2148	The inferred metagenomes from 16S data using PICRUSt demonstrated that decreased metabolic genes, such as through biosynthesis of siderophore group nonribosomal peptides, non-homologous end-joining, and glycosphingolipid biosynthesis of lacto and neolacto series, may maintain microbiota homeostasis during inflammation from IN treatment in DSS-induced colitis.
31726738	4	32	theme	inflammation	890:901	arg1	cytokines					903:911	inflammation cytokines	890:911	inflammation cytokines	890:911	To investigate the relationships of IN, colitis, and gut microbiomes, a dextran sulfate sodium (DSS)-induced mice colitis model was tested to explore the protective effects of IN on macroscopic colitis symptoms, the histopathological structure, inflammation cytokines, and gut microbiota, and their potential functions.
31726738	10	33	theme	group	2094:2098	arg1	peptides					2113:2120	siderophore group nonribosomal peptides	2082:2120	siderophore group nonribosomal peptides	2082:2120	The inferred metagenomes from 16S data using PICRUSt demonstrated that decreased metabolic genes, such as through biosynthesis of siderophore group nonribosomal peptides, non-homologous end-joining, and glycosphingolipid biosynthesis of lacto and neolacto series, may maintain microbiota homeostasis during inflammation from IN treatment in DSS-induced colitis.
31726738	1	34	theme	traditional	154:164	arg1	medicine					174:181	a traditional Chinese medicine	152:181	a traditional Chinese medicine	152:181	Indigo naturalis (IN) is a traditional Chinese medicine, named Qing-Dai, which is extracted from indigo plants and has been used to treat patients with inflammatory bowel disease (IBD) in China and Japan.
31726738	1	34	theme	traditional	154:164	arg1	naturalis					134:142	Indigo naturalis	127:142	Indigo naturalis (IN)	127:147	Indigo naturalis (IN) is a traditional Chinese medicine, named Qing-Dai, which is extracted from indigo plants and has been used to treat patients with inflammatory bowel disease (IBD) in China and Japan.
31726738	7	35	link	enzyme-linked	1266:1278	arg1	ELISA					1301:1305	ELISA	1301:1305	ELISA	1301:1305	The clinical activity score (CAS), hematoxylin and eosin (H&E) staining results, and enzyme-linked immunosorbent assay (ELISA) results in this study showed that IN greatly improved the health conditions of the tested colitis mice, ameliorated the histopathological structure of the colon tissue, down-regulated pro-inflammatory cytokines, and up-regulated anti-inflammatory cytokines.
31726738	7	35	link	enzyme-linked	1266:1278	arg1	assay					1294:1298	enzyme-linked immunosorbent assay	1266:1298	enzyme-linked immunosorbent assay (ELISA)	1266:1306	The clinical activity score (CAS), hematoxylin and eosin (H&E) staining results, and enzyme-linked immunosorbent assay (ELISA) results in this study showed that IN greatly improved the health conditions of the tested colitis mice, ameliorated the histopathological structure of the colon tissue, down-regulated pro-inflammatory cytokines, and up-regulated anti-inflammatory cytokines.
31726738	9	36	theme	gram-positive	1896:1908	arg1	bacteria					1910:1917	the anaerobic gram-positive bacteria	1882:1917	the anaerobic gram-positive bacteria of Turicibacter	1882:1933	The therapeutic effect of IN may be closely related to the anaerobic gram-positive bacteria of Turicibacter and Peptococcus.
31726738	0	37	theme	Intestinal	94:103	arg1	Community					116:124	the Intestinal Microbiota Community	90:124	the Intestinal Microbiota Community	90:124	Indigo Naturalis Ameliorates Dextran Sulfate Sodium-Induced Colitis in Mice by Modulating the Intestinal Microbiota Community.
31726738	8	38	theme	microbiota	1691:1700	arg1	balance					1676:1682	the balance	1672:1682	the balance of gut microbiota	1672:1700	The results of 16S rDNA sequences analysis with the Illumina MiSeq platform showed that IN could modulate the balance of gut microbiota, especially by down-regulating the relative quantity of Turicibacter and up-regulating the relative quantity of Peptococcus.
31726738	7	39	theme	colitis	1398:1404	arg1	mice					1406:1409	the tested colitis mice	1387:1409	the tested colitis mice	1387:1409	The clinical activity score (CAS), hematoxylin and eosin (H&E) staining results, and enzyme-linked immunosorbent assay (ELISA) results in this study showed that IN greatly improved the health conditions of the tested colitis mice, ameliorated the histopathological structure of the colon tissue, down-regulated pro-inflammatory cytokines, and up-regulated anti-inflammatory cytokines.
31726738	0	40	from	Colitis	60:66	arg1	Mice					71:74	Mice	71:74	Mice	71:74	Indigo Naturalis Ameliorates Dextran Sulfate Sodium-Induced Colitis in Mice by Modulating the Intestinal Microbiota Community.
31726738	10	41	theme	non-homologous	2123:2136	arg1	end-joining					2138:2148	non-homologous end-joining	2123:2148	non-homologous end-joining	2123:2148	The inferred metagenomes from 16S data using PICRUSt demonstrated that decreased metabolic genes, such as through biosynthesis of siderophore group nonribosomal peptides, non-homologous end-joining, and glycosphingolipid biosynthesis of lacto and neolacto series, may maintain microbiota homeostasis during inflammation from IN treatment in DSS-induced colitis.
31726738	4	42	theme	gut	698:700	arg1	microbiomes					702:712	IN, colitis, and gut microbiomes	681:712	IN, colitis, and gut microbiomes	681:712	To investigate the relationships of IN, colitis, and gut microbiomes, a dextran sulfate sodium (DSS)-induced mice colitis model was tested to explore the protective effects of IN on macroscopic colitis symptoms, the histopathological structure, inflammation cytokines, and gut microbiota, and their potential functions.
31726738	10	43	from	data	1986:1989	arg1	metagenomes					1965:1975	The inferred metagenomes	1952:1975	The inferred metagenomes from 16S data using PICRUSt	1952:2003	The inferred metagenomes from 16S data using PICRUSt demonstrated that decreased metabolic genes, such as through biosynthesis of siderophore group nonribosomal peptides, non-homologous end-joining, and glycosphingolipid biosynthesis of lacto and neolacto series, may maintain microbiota homeostasis during inflammation from IN treatment in DSS-induced colitis.
31726738	0	44	theme	Indigo	0:5	arg1	Naturalis					7:15	Indigo Naturalis	0:15	Indigo Naturalis	0:15	Indigo Naturalis Ameliorates Dextran Sulfate Sodium-Induced Colitis in Mice by Modulating the Intestinal Microbiota Community.
31726738	7	45	theme	score	1203:1207	arg1	results					1308:1314	The clinical activity score (CAS), hematoxylin and eosin (H&E) staining results, and enzyme-linked immunosorbent assay (ELISA) results	1181:1314	The clinical activity score (CAS), hematoxylin and eosin (H&E) staining results, and enzyme-linked immunosorbent assay (ELISA) results in this study	1181:1328	The clinical activity score (CAS), hematoxylin and eosin (H&E) staining results, and enzyme-linked immunosorbent assay (ELISA) results in this study showed that IN greatly improved the health conditions of the tested colitis mice, ameliorated the histopathological structure of the colon tissue, down-regulated pro-inflammatory cytokines, and up-regulated anti-inflammatory cytokines.
31726738	10	46	theme	glycosphingolipid	2155:2171	arg1	biosynthesis					2173:2184	glycosphingolipid biosynthesis	2155:2184	glycosphingolipid biosynthesis of lacto and neolacto series	2155:2213	The inferred metagenomes from 16S data using PICRUSt demonstrated that decreased metabolic genes, such as through biosynthesis of siderophore group nonribosomal peptides, non-homologous end-joining, and glycosphingolipid biosynthesis of lacto and neolacto series, may maintain microbiota homeostasis during inflammation from IN treatment in DSS-induced colitis.
31726738	1	47	used	used	251:254	arg2	naturalis					134:142	Indigo naturalis	127:142	Indigo naturalis (IN)	127:147	Indigo naturalis (IN) is a traditional Chinese medicine, named Qing-Dai, which is extracted from indigo plants and has been used to treat patients with inflammatory bowel disease (IBD) in China and Japan.
31726738	1	47	used	used	251:254	arg2	medicine					174:181	a traditional Chinese medicine	152:181	a traditional Chinese medicine	152:181	Indigo naturalis (IN) is a traditional Chinese medicine, named Qing-Dai, which is extracted from indigo plants and has been used to treat patients with inflammatory bowel disease (IBD) in China and Japan.
31726738	7	48	theme	clinical	1185:1192	arg1	score					1203:1207	The clinical activity score	1181:1207	The clinical activity score (CAS)	1181:1213	The clinical activity score (CAS), hematoxylin and eosin (H&E) staining results, and enzyme-linked immunosorbent assay (ELISA) results in this study showed that IN greatly improved the health conditions of the tested colitis mice, ameliorated the histopathological structure of the colon tissue, down-regulated pro-inflammatory cytokines, and up-regulated anti-inflammatory cytokines.
31726738	7	48	theme	clinical	1185:1192	arg1	CAS					1210:1212	CAS	1210:1212	CAS	1210:1212	The clinical activity score (CAS), hematoxylin and eosin (H&E) staining results, and enzyme-linked immunosorbent assay (ELISA) results in this study showed that IN greatly improved the health conditions of the tested colitis mice, ameliorated the histopathological structure of the colon tissue, down-regulated pro-inflammatory cytokines, and up-regulated anti-inflammatory cytokines.
31726738	3	49	theme	IN	587:588	arg1	action					577:582	the protective action	562:582	the protective action of IN on colitis	562:599	Regarding the significance of alterations of intestinal flora related to IBD and the poor water solubility of the blue IN powder, we predicted that the protective action of IN on colitis may occur through modifying gut microbiota.
31726738	2	50	theme	IN	368:369	arg1	effects					357:363	notable effects	349:363	notable effects of IN on colitis	349:380	Though there are notable effects of IN on colitis, the mechanisms remain elusive.
31726738	0	51	theme	Sulfate	37:43	arg1	Colitis					60:66	Dextran Sulfate Sodium-Induced Colitis	29:66	Dextran Sulfate Sodium-Induced Colitis in Mice	29:74	Indigo Naturalis Ameliorates Dextran Sulfate Sodium-Induced Colitis in Mice by Modulating the Intestinal Microbiota Community.
31726738	1	52	theme	inflammatory	279:290	arg1	IBD					307:309	IBD	307:309	IBD	307:309	Indigo naturalis (IN) is a traditional Chinese medicine, named Qing-Dai, which is extracted from indigo plants and has been used to treat patients with inflammatory bowel disease (IBD) in China and Japan.
31726738	1	52	theme	inflammatory	279:290	arg1	disease					298:304	inflammatory bowel disease	279:304	inflammatory bowel disease (IBD)	279:310	Indigo naturalis (IN) is a traditional Chinese medicine, named Qing-Dai, which is extracted from indigo plants and has been used to treat patients with inflammatory bowel disease (IBD) in China and Japan.
31726738	7	53	theme	health	1366:1371	arg1	conditions					1373:1382	the health conditions	1362:1382	the health conditions of the tested colitis mice	1362:1409	The clinical activity score (CAS), hematoxylin and eosin (H&E) staining results, and enzyme-linked immunosorbent assay (ELISA) results in this study showed that IN greatly improved the health conditions of the tested colitis mice, ameliorated the histopathological structure of the colon tissue, down-regulated pro-inflammatory cytokines, and up-regulated anti-inflammatory cytokines.
31726738	4	54	theme	gut	918:920	arg1	microbiota					922:931	gut microbiota	918:931	gut microbiota	918:931	To investigate the relationships of IN, colitis, and gut microbiomes, a dextran sulfate sodium (DSS)-induced mice colitis model was tested to explore the protective effects of IN on macroscopic colitis symptoms, the histopathological structure, inflammation cytokines, and gut microbiota, and their potential functions.
31726738	3	55	theme	alterations	444:454	arg1	solubility					510:519	the poor water solubility	495:519	the poor water solubility of the blue IN powder	495:541	Regarding the significance of alterations of intestinal flora related to IBD and the poor water solubility of the blue IN powder, we predicted that the protective action of IN on colitis may occur through modifying gut microbiota.
31726738	3	55	theme	alterations	444:454	arg1	significance					428:439	the significance	424:439	the significance of alterations of intestinal flora related to IBD	424:489	Regarding the significance of alterations of intestinal flora related to IBD and the poor water solubility of the blue IN powder, we predicted that the protective action of IN on colitis may occur through modifying gut microbiota.
31726738	2	56	from	effects	357:363	arg1	colitis					374:380	colitis	374:380	colitis	374:380	Though there are notable effects of IN on colitis, the mechanisms remain elusive.
31726738	10	57	theme	inferred	1956:1963	arg1	metagenomes					1965:1975	The inferred metagenomes	1952:1975	The inferred metagenomes from 16S data using PICRUSt	1952:2003	The inferred metagenomes from 16S data using PICRUSt demonstrated that decreased metabolic genes, such as through biosynthesis of siderophore group nonribosomal peptides, non-homologous end-joining, and glycosphingolipid biosynthesis of lacto and neolacto series, may maintain microbiota homeostasis during inflammation from IN treatment in DSS-induced colitis.
31726738	3	58	theme	related	476:482	arg1	flora					470:474	intestinal flora	459:474	intestinal flora related to IBD	459:489	Regarding the significance of alterations of intestinal flora related to IBD and the poor water solubility of the blue IN powder, we predicted that the protective action of IN on colitis may occur through modifying gut microbiota.
31726738	1	59	theme	indigo	224:229	arg1	plants					231:236	indigo plants	224:236	indigo plants	224:236	Indigo naturalis (IN) is a traditional Chinese medicine, named Qing-Dai, which is extracted from indigo plants and has been used to treat patients with inflammatory bowel disease (IBD) in China and Japan.
31726738	8	60	theme	relative	1737:1744	arg1	quantity					1746:1753	the relative quantity	1733:1753	the relative quantity of Turicibacter	1733:1769	The results of 16S rDNA sequences analysis with the Illumina MiSeq platform showed that IN could modulate the balance of gut microbiota, especially by down-regulating the relative quantity of Turicibacter and up-regulating the relative quantity of Peptococcus.
31726738	3	61	theme	intestinal	459:468	arg1	flora					470:474	intestinal flora	459:474	intestinal flora related to IBD	459:489	Regarding the significance of alterations of intestinal flora related to IBD and the poor water solubility of the blue IN powder, we predicted that the protective action of IN on colitis may occur through modifying gut microbiota.
31726738	10	62	theme	series	2208:2213	arg1	biosynthesis					2173:2184	glycosphingolipid biosynthesis	2155:2184	glycosphingolipid biosynthesis of lacto and neolacto series	2155:2213	The inferred metagenomes from 16S data using PICRUSt demonstrated that decreased metabolic genes, such as through biosynthesis of siderophore group nonribosomal peptides, non-homologous end-joining, and glycosphingolipid biosynthesis of lacto and neolacto series, may maintain microbiota homeostasis during inflammation from IN treatment in DSS-induced colitis.
31726738	10	62	theme	series	2208:2213	arg1	biosynthesis					2066:2077	biosynthesis	2066:2077	biosynthesis of siderophore group nonribosomal peptides	2066:2120	The inferred metagenomes from 16S data using PICRUSt demonstrated that decreased metabolic genes, such as through biosynthesis of siderophore group nonribosomal peptides, non-homologous end-joining, and glycosphingolipid biosynthesis of lacto and neolacto series, may maintain microbiota homeostasis during inflammation from IN treatment in DSS-induced colitis.
31726738	10	62	theme	series	2208:2213	arg1	end-joining					2138:2148	non-homologous end-joining	2123:2148	non-homologous end-joining	2123:2148	The inferred metagenomes from 16S data using PICRUSt demonstrated that decreased metabolic genes, such as through biosynthesis of siderophore group nonribosomal peptides, non-homologous end-joining, and glycosphingolipid biosynthesis of lacto and neolacto series, may maintain microbiota homeostasis during inflammation from IN treatment in DSS-induced colitis.
31726738	10	63	from	treatment	2280:2288	arg1	colitis					2305:2311	DSS-induced colitis	2293:2311	DSS-induced colitis	2293:2311	The inferred metagenomes from 16S data using PICRUSt demonstrated that decreased metabolic genes, such as through biosynthesis of siderophore group nonribosomal peptides, non-homologous end-joining, and glycosphingolipid biosynthesis of lacto and neolacto series, may maintain microbiota homeostasis during inflammation from IN treatment in DSS-induced colitis.
31726738	10	64	theme	metabolic	2033:2041	arg1	biosynthesis					2173:2184	glycosphingolipid biosynthesis	2155:2184	glycosphingolipid biosynthesis of lacto and neolacto series	2155:2213	The inferred metagenomes from 16S data using PICRUSt demonstrated that decreased metabolic genes, such as through biosynthesis of siderophore group nonribosomal peptides, non-homologous end-joining, and glycosphingolipid biosynthesis of lacto and neolacto series, may maintain microbiota homeostasis during inflammation from IN treatment in DSS-induced colitis.
31726738	10	64	theme	metabolic	2033:2041	arg1	biosynthesis					2066:2077	biosynthesis	2066:2077	biosynthesis of siderophore group nonribosomal peptides	2066:2120	The inferred metagenomes from 16S data using PICRUSt demonstrated that decreased metabolic genes, such as through biosynthesis of siderophore group nonribosomal peptides, non-homologous end-joining, and glycosphingolipid biosynthesis of lacto and neolacto series, may maintain microbiota homeostasis during inflammation from IN treatment in DSS-induced colitis.
31726738	10	64	theme	metabolic	2033:2041	arg1	genes					2043:2047	decreased metabolic genes	2023:2047	decreased metabolic genes	2023:2047	The inferred metagenomes from 16S data using PICRUSt demonstrated that decreased metabolic genes, such as through biosynthesis of siderophore group nonribosomal peptides, non-homologous end-joining, and glycosphingolipid biosynthesis of lacto and neolacto series, may maintain microbiota homeostasis during inflammation from IN treatment in DSS-induced colitis.
31726738	10	64	theme	metabolic	2033:2041	arg1	end-joining					2138:2148	non-homologous end-joining	2123:2148	non-homologous end-joining	2123:2148	The inferred metagenomes from 16S data using PICRUSt demonstrated that decreased metabolic genes, such as through biosynthesis of siderophore group nonribosomal peptides, non-homologous end-joining, and glycosphingolipid biosynthesis of lacto and neolacto series, may maintain microbiota homeostasis during inflammation from IN treatment in DSS-induced colitis.
31726738	6	65	theme	ultra-performance	1133:1149	arg1	chromatography					1158:1171	ultra-performance liquid chromatography	1133:1171	ultra-performance liquid chromatography (UPLC)	1133:1178	Firstly, because it was a mixture, the main chemical compositions of indigo and indirubin in IN were detected by ultra-performance liquid chromatography (UPLC).
31726738	6	65	theme	ultra-performance	1133:1149	arg1	UPLC					1174:1177	UPLC	1174:1177	UPLC	1174:1177	Firstly, because it was a mixture, the main chemical compositions of indigo and indirubin in IN were detected by ultra-performance liquid chromatography (UPLC).
31726738	1	66	theme	Chinese	166:172	arg1	medicine					174:181	a traditional Chinese medicine	152:181	a traditional Chinese medicine	152:181	Indigo naturalis (IN) is a traditional Chinese medicine, named Qing-Dai, which is extracted from indigo plants and has been used to treat patients with inflammatory bowel disease (IBD) in China and Japan.
31726738	1	66	theme	Chinese	166:172	arg1	naturalis					134:142	Indigo naturalis	127:142	Indigo naturalis (IN)	127:147	Indigo naturalis (IN) is a traditional Chinese medicine, named Qing-Dai, which is extracted from indigo plants and has been used to treat patients with inflammatory bowel disease (IBD) in China and Japan.
31726738	10	67	theme	microbiota	2229:2238	arg1	homeostasis					2240:2250	microbiota homeostasis	2229:2250	microbiota homeostasis	2229:2250	The inferred metagenomes from 16S data using PICRUSt demonstrated that decreased metabolic genes, such as through biosynthesis of siderophore group nonribosomal peptides, non-homologous end-joining, and glycosphingolipid biosynthesis of lacto and neolacto series, may maintain microbiota homeostasis during inflammation from IN treatment in DSS-induced colitis.
31726738	1	68	with	patients	265:272	arg1	IBD					307:309	IBD	307:309	IBD	307:309	Indigo naturalis (IN) is a traditional Chinese medicine, named Qing-Dai, which is extracted from indigo plants and has been used to treat patients with inflammatory bowel disease (IBD) in China and Japan.
31726738	1	68	with	patients	265:272	arg1	disease					298:304	inflammatory bowel disease	279:304	inflammatory bowel disease (IBD)	279:310	Indigo naturalis (IN) is a traditional Chinese medicine, named Qing-Dai, which is extracted from indigo plants and has been used to treat patients with inflammatory bowel disease (IBD) in China and Japan.
31726738	7	69	theme	immunosorbent	1280:1292	arg1	ELISA					1301:1305	ELISA	1301:1305	ELISA	1301:1305	The clinical activity score (CAS), hematoxylin and eosin (H&E) staining results, and enzyme-linked immunosorbent assay (ELISA) results in this study showed that IN greatly improved the health conditions of the tested colitis mice, ameliorated the histopathological structure of the colon tissue, down-regulated pro-inflammatory cytokines, and up-regulated anti-inflammatory cytokines.
31726738	7	69	theme	immunosorbent	1280:1292	arg1	assay					1294:1298	enzyme-linked immunosorbent assay	1266:1298	enzyme-linked immunosorbent assay (ELISA)	1266:1306	The clinical activity score (CAS), hematoxylin and eosin (H&E) staining results, and enzyme-linked immunosorbent assay (ELISA) results in this study showed that IN greatly improved the health conditions of the tested colitis mice, ameliorated the histopathological structure of the colon tissue, down-regulated pro-inflammatory cytokines, and up-regulated anti-inflammatory cytokines.
31726738	8	70	theme	rDNA	1585:1588	arg1	sequences					1590:1598	16S rDNA sequences	1581:1598	16S rDNA sequences analysis with the Illumina MiSeq platform	1581:1640	The results of 16S rDNA sequences analysis with the Illumina MiSeq platform showed that IN could modulate the balance of gut microbiota, especially by down-regulating the relative quantity of Turicibacter and up-regulating the relative quantity of Peptococcus.
31726738	7	71	theme	pro-inflammatory	1492:1507	arg1	cytokines					1509:1517	pro-inflammatory cytokines	1492:1517	pro-inflammatory cytokines	1492:1517	The clinical activity score (CAS), hematoxylin and eosin (H&E) staining results, and enzyme-linked immunosorbent assay (ELISA) results in this study showed that IN greatly improved the health conditions of the tested colitis mice, ameliorated the histopathological structure of the colon tissue, down-regulated pro-inflammatory cytokines, and up-regulated anti-inflammatory cytokines.
31726738	4	72	theme	mice	754:757	arg1	model					767:771	a dextran sulfate sodium (DSS)-induced mice colitis model	715:771	a dextran sulfate sodium (DSS)-induced mice colitis model	715:771	To investigate the relationships of IN, colitis, and gut microbiomes, a dextran sulfate sodium (DSS)-induced mice colitis model was tested to explore the protective effects of IN on macroscopic colitis symptoms, the histopathological structure, inflammation cytokines, and gut microbiota, and their potential functions.
31726738	3	73	theme	water	504:508	arg1	solubility					510:519	the poor water solubility	495:519	the poor water solubility of the blue IN powder	495:541	Regarding the significance of alterations of intestinal flora related to IBD and the poor water solubility of the blue IN powder, we predicted that the protective action of IN on colitis may occur through modifying gut microbiota.
31726738	7	74	theme	results	1253:1259	arg1	results					1308:1314	The clinical activity score (CAS), hematoxylin and eosin (H&E) staining results, and enzyme-linked immunosorbent assay (ELISA) results	1181:1314	The clinical activity score (CAS), hematoxylin and eosin (H&E) staining results, and enzyme-linked immunosorbent assay (ELISA) results in this study	1181:1328	The clinical activity score (CAS), hematoxylin and eosin (H&E) staining results, and enzyme-linked immunosorbent assay (ELISA) results in this study showed that IN greatly improved the health conditions of the tested colitis mice, ameliorated the histopathological structure of the colon tissue, down-regulated pro-inflammatory cytokines, and up-regulated anti-inflammatory cytokines.
31726738	9	75	theme	therapeutic	1831:1841	arg1	related					1871:1877	related	1871:1877	related	1871:1877	The therapeutic effect of IN may be closely related to the anaerobic gram-positive bacteria of Turicibacter and Peptococcus.
31726738	9	75	theme	therapeutic	1831:1841	arg1	effect					1843:1848	The therapeutic effect	1827:1848	The therapeutic effect of IN	1827:1854	The therapeutic effect of IN may be closely related to the anaerobic gram-positive bacteria of Turicibacter and Peptococcus.
31726738	8	76	theme	Peptococcus	1814:1824	arg1	quantity					1802:1809	the relative quantity	1789:1809	the relative quantity of Peptococcus	1789:1824	The results of 16S rDNA sequences analysis with the Illumina MiSeq platform showed that IN could modulate the balance of gut microbiota, especially by down-regulating the relative quantity of Turicibacter and up-regulating the relative quantity of Peptococcus.
31726738	7	77	theme	colon	1463:1467	arg1	tissue					1469:1474	the colon tissue	1459:1474	the colon tissue	1459:1474	The clinical activity score (CAS), hematoxylin and eosin (H&E) staining results, and enzyme-linked immunosorbent assay (ELISA) results in this study showed that IN greatly improved the health conditions of the tested colitis mice, ameliorated the histopathological structure of the colon tissue, down-regulated pro-inflammatory cytokines, and up-regulated anti-inflammatory cytokines.
31726738	10	78	theme	siderophore	2082:2092	arg1	peptides					2113:2120	siderophore group nonribosomal peptides	2082:2120	siderophore group nonribosomal peptides	2082:2120	The inferred metagenomes from 16S data using PICRUSt demonstrated that decreased metabolic genes, such as through biosynthesis of siderophore group nonribosomal peptides, non-homologous end-joining, and glycosphingolipid biosynthesis of lacto and neolacto series, may maintain microbiota homeostasis during inflammation from IN treatment in DSS-induced colitis.
31726738	3	79	theme	blue	528:531	arg1	solubility					510:519	the poor water solubility	495:519	the poor water solubility of the blue IN powder	495:541	Regarding the significance of alterations of intestinal flora related to IBD and the poor water solubility of the blue IN powder, we predicted that the protective action of IN on colitis may occur through modifying gut microbiota.
31726738	3	79	theme	blue	528:531	arg1	significance					428:439	the significance	424:439	the significance of alterations of intestinal flora related to IBD	424:489	Regarding the significance of alterations of intestinal flora related to IBD and the poor water solubility of the blue IN powder, we predicted that the protective action of IN on colitis may occur through modifying gut microbiota.
31726738	8	80	theme	MiSeq	1627:1631	arg1	platform					1633:1640	the Illumina MiSeq platform	1614:1640	the Illumina MiSeq platform	1614:1640	The results of 16S rDNA sequences analysis with the Illumina MiSeq platform showed that IN could modulate the balance of gut microbiota, especially by down-regulating the relative quantity of Turicibacter and up-regulating the relative quantity of Peptococcus.
31726738	1	81	theme	Indigo	127:132	arg1	IN					145:146	IN	145:146	IN	145:146	Indigo naturalis (IN) is a traditional Chinese medicine, named Qing-Dai, which is extracted from indigo plants and has been used to treat patients with inflammatory bowel disease (IBD) in China and Japan.
31726738	1	81	theme	Indigo	127:132	arg1	naturalis					134:142	Indigo naturalis	127:142	Indigo naturalis (IN)	127:147	Indigo naturalis (IN) is a traditional Chinese medicine, named Qing-Dai, which is extracted from indigo plants and has been used to treat patients with inflammatory bowel disease (IBD) in China and Japan.
31726738	1	81	theme	Indigo	127:132	arg1	medicine					174:181	a traditional Chinese medicine	152:181	a traditional Chinese medicine	152:181	Indigo naturalis (IN) is a traditional Chinese medicine, named Qing-Dai, which is extracted from indigo plants and has been used to treat patients with inflammatory bowel disease (IBD) in China and Japan.
31726738	10	82	theme	DSS-induced	2293:2303	arg1	colitis					2305:2311	DSS-induced colitis	2293:2311	DSS-induced colitis	2293:2311	The inferred metagenomes from 16S data using PICRUSt demonstrated that decreased metabolic genes, such as through biosynthesis of siderophore group nonribosomal peptides, non-homologous end-joining, and glycosphingolipid biosynthesis of lacto and neolacto series, may maintain microbiota homeostasis during inflammation from IN treatment in DSS-induced colitis.
31726738	7	83	theme	histopathological	1428:1444	arg1	structure					1446:1454	the histopathological structure	1424:1454	the histopathological structure of the colon tissue	1424:1474	The clinical activity score (CAS), hematoxylin and eosin (H&E) staining results, and enzyme-linked immunosorbent assay (ELISA) results in this study showed that IN greatly improved the health conditions of the tested colitis mice, ameliorated the histopathological structure of the colon tissue, down-regulated pro-inflammatory cytokines, and up-regulated anti-inflammatory cytokines.
31726738	4	84	theme	protective	799:808	arg1	effects					810:816	the protective effects	795:816	the protective effects of IN on macroscopic colitis symptoms, the histopathological structure, inflammation cytokines, and gut microbiota, and their potential functions	795:962	To investigate the relationships of IN, colitis, and gut microbiomes, a dextran sulfate sodium (DSS)-induced mice colitis model was tested to explore the protective effects of IN on macroscopic colitis symptoms, the histopathological structure, inflammation cytokines, and gut microbiota, and their potential functions.
31726738	4	85	theme	histopathological	861:877	arg1	structure					879:887	the histopathological structure	857:887	the histopathological structure	857:887	To investigate the relationships of IN, colitis, and gut microbiomes, a dextran sulfate sodium (DSS)-induced mice colitis model was tested to explore the protective effects of IN on macroscopic colitis symptoms, the histopathological structure, inflammation cytokines, and gut microbiota, and their potential functions.
31726738	6	86	theme	chemical	1064:1071	arg1	compositions					1073:1084	the main chemical compositions	1055:1084	the main chemical compositions of indigo and indirubin in IN	1055:1114	Firstly, because it was a mixture, the main chemical compositions of indigo and indirubin in IN were detected by ultra-performance liquid chromatography (UPLC).
31726738	10	87	theme	nonribosomal	2100:2111	arg1	peptides					2113:2120	siderophore group nonribosomal peptides	2082:2120	siderophore group nonribosomal peptides	2082:2120	The inferred metagenomes from 16S data using PICRUSt demonstrated that decreased metabolic genes, such as through biosynthesis of siderophore group nonribosomal peptides, non-homologous end-joining, and glycosphingolipid biosynthesis of lacto and neolacto series, may maintain microbiota homeostasis during inflammation from IN treatment in DSS-induced colitis.
31726738	6	88	from	compositions	1073:1084	arg1	IN					1113:1114	IN	1113:1114	IN	1113:1114	Firstly, because it was a mixture, the main chemical compositions of indigo and indirubin in IN were detected by ultra-performance liquid chromatography (UPLC).
31726738	5	89	used	used	990:993	arg2	SASP					980:983	SASP	980:983	SASP	980:983	Sulfasalazine (SASP) was used as the positive control.
31726738	5	89	used	used	990:993	arg2	Sulfasalazine					965:977	Sulfasalazine	965:977	Sulfasalazine (SASP)	965:984	Sulfasalazine (SASP) was used as the positive control.
31726738	5	89	used	used	990:993	arg2	control					1011:1017	the positive control	998:1017	the positive control	998:1017	Sulfasalazine (SASP) was used as the positive control.
31726738	7	90	theme	mice	1406:1409	arg1	conditions					1373:1382	the health conditions	1362:1382	the health conditions of the tested colitis mice	1362:1409	The clinical activity score (CAS), hematoxylin and eosin (H&E) staining results, and enzyme-linked immunosorbent assay (ELISA) results in this study showed that IN greatly improved the health conditions of the tested colitis mice, ameliorated the histopathological structure of the colon tissue, down-regulated pro-inflammatory cytokines, and up-regulated anti-inflammatory cytokines.
31726738	9	91	theme	anaerobic	1886:1894	arg1	bacteria					1910:1917	the anaerobic gram-positive bacteria	1882:1917	the anaerobic gram-positive bacteria of Turicibacter	1882:1933	The therapeutic effect of IN may be closely related to the anaerobic gram-positive bacteria of Turicibacter and Peptococcus.
31726738	0	92	theme	Microbiota	105:114	arg1	Community					116:124	the Intestinal Microbiota Community	90:124	the Intestinal Microbiota Community	90:124	Indigo Naturalis Ameliorates Dextran Sulfate Sodium-Induced Colitis in Mice by Modulating the Intestinal Microbiota Community.
31726738	7	93	theme	tested	1391:1396	arg1	mice					1406:1409	the tested colitis mice	1387:1409	the tested colitis mice	1387:1409	The clinical activity score (CAS), hematoxylin and eosin (H&E) staining results, and enzyme-linked immunosorbent assay (ELISA) results in this study showed that IN greatly improved the health conditions of the tested colitis mice, ameliorated the histopathological structure of the colon tissue, down-regulated pro-inflammatory cytokines, and up-regulated anti-inflammatory cytokines.
31726738	4	94	theme	colitis	839:845	arg1	symptoms					847:854	macroscopic colitis symptoms	827:854	macroscopic colitis symptoms	827:854	To investigate the relationships of IN, colitis, and gut microbiomes, a dextran sulfate sodium (DSS)-induced mice colitis model was tested to explore the protective effects of IN on macroscopic colitis symptoms, the histopathological structure, inflammation cytokines, and gut microbiota, and their potential functions.
31726738	4	95	theme	colitis	685:691	arg1	microbiomes					702:712	IN, colitis, and gut microbiomes	681:712	IN, colitis, and gut microbiomes	681:712	To investigate the relationships of IN, colitis, and gut microbiomes, a dextran sulfate sodium (DSS)-induced mice colitis model was tested to explore the protective effects of IN on macroscopic colitis symptoms, the histopathological structure, inflammation cytokines, and gut microbiota, and their potential functions.
31726738	8	96	theme	gut	1687:1689	arg1	microbiota					1691:1700	gut microbiota	1687:1700	gut microbiota	1687:1700	The results of 16S rDNA sequences analysis with the Illumina MiSeq platform showed that IN could modulate the balance of gut microbiota, especially by down-regulating the relative quantity of Turicibacter and up-regulating the relative quantity of Peptococcus.
31726738	8	97	dep	sequences	1590:1598	arg1	analysis					1600:1607	analysis	1600:1607	analysis	1600:1607	The results of 16S rDNA sequences analysis with the Illumina MiSeq platform showed that IN could modulate the balance of gut microbiota, especially by down-regulating the relative quantity of Turicibacter and up-regulating the relative quantity of Peptococcus.
31726738	2	98	theme	notable	349:355	arg1	effects					357:363	notable effects	349:363	notable effects of IN on colitis	349:380	Though there are notable effects of IN on colitis, the mechanisms remain elusive.
31726738	5	99	theme	positive	1002:1009	arg1	Sulfasalazine					965:977	Sulfasalazine	965:977	Sulfasalazine (SASP)	965:984	Sulfasalazine (SASP) was used as the positive control.
31726738	5	99	theme	positive	1002:1009	arg1	control					1011:1017	the positive control	998:1017	the positive control	998:1017	Sulfasalazine (SASP) was used as the positive control.
31726738	7	100	theme	activity	1194:1201	arg1	score					1203:1207	The clinical activity score	1181:1207	The clinical activity score (CAS)	1181:1213	The clinical activity score (CAS), hematoxylin and eosin (H&E) staining results, and enzyme-linked immunosorbent assay (ELISA) results in this study showed that IN greatly improved the health conditions of the tested colitis mice, ameliorated the histopathological structure of the colon tissue, down-regulated pro-inflammatory cytokines, and up-regulated anti-inflammatory cytokines.
31726738	7	100	theme	activity	1194:1201	arg1	CAS					1210:1212	CAS	1210:1212	CAS	1210:1212	The clinical activity score (CAS), hematoxylin and eosin (H&E) staining results, and enzyme-linked immunosorbent assay (ELISA) results in this study showed that IN greatly improved the health conditions of the tested colitis mice, ameliorated the histopathological structure of the colon tissue, down-regulated pro-inflammatory cytokines, and up-regulated anti-inflammatory cytokines.
31726738	8	101	with	analysis	1600:1607	arg1	platform					1633:1640	the Illumina MiSeq platform	1614:1640	the Illumina MiSeq platform	1614:1640	The results of 16S rDNA sequences analysis with the Illumina MiSeq platform showed that IN could modulate the balance of gut microbiota, especially by down-regulating the relative quantity of Turicibacter and up-regulating the relative quantity of Peptococcus.
31726738	3	102	from	blue	528:531	arg1	powder					536:541	powder	536:541	powder	536:541	Regarding the significance of alterations of intestinal flora related to IBD and the poor water solubility of the blue IN powder, we predicted that the protective action of IN on colitis may occur through modifying gut microbiota.
31726738	0	103	theme	Dextran	29:35	arg1	Sulfate					37:43	Dextran Sulfate	29:43	Dextran Sulfate Sodium-Induced Colitis in Mice	29:74	Indigo Naturalis Ameliorates Dextran Sulfate Sodium-Induced Colitis in Mice by Modulating the Intestinal Microbiota Community.
31726738	7	104	theme	anti-inflammatory	1537:1553	arg1	cytokines					1555:1563	anti-inflammatory cytokines	1537:1563	anti-inflammatory cytokines	1537:1563	The clinical activity score (CAS), hematoxylin and eosin (H&E) staining results, and enzyme-linked immunosorbent assay (ELISA) results in this study showed that IN greatly improved the health conditions of the tested colitis mice, ameliorated the histopathological structure of the colon tissue, down-regulated pro-inflammatory cytokines, and up-regulated anti-inflammatory cytokines.
31726738	4	105	theme	IN	681:682	arg1	microbiomes					702:712	IN, colitis, and gut microbiomes	681:712	IN, colitis, and gut microbiomes	681:712	To investigate the relationships of IN, colitis, and gut microbiomes, a dextran sulfate sodium (DSS)-induced mice colitis model was tested to explore the protective effects of IN on macroscopic colitis symptoms, the histopathological structure, inflammation cytokines, and gut microbiota, and their potential functions.
31726738	3	106	from	powder	536:541	arg1	solubility					510:519	the poor water solubility	495:519	the poor water solubility of the blue IN powder	495:541	Regarding the significance of alterations of intestinal flora related to IBD and the poor water solubility of the blue IN powder, we predicted that the protective action of IN on colitis may occur through modifying gut microbiota.
31726738	3	106	from	powder	536:541	arg1	significance					428:439	the significance	424:439	the significance of alterations of intestinal flora related to IBD	424:489	Regarding the significance of alterations of intestinal flora related to IBD and the poor water solubility of the blue IN powder, we predicted that the protective action of IN on colitis may occur through modifying gut microbiota.
31726738	3	106	from	powder	536:541	arg1	blue					528:531	blue	528:531	blue	528:531	Regarding the significance of alterations of intestinal flora related to IBD and the poor water solubility of the blue IN powder, we predicted that the protective action of IN on colitis may occur through modifying gut microbiota.
31726738	0	107	theme	Sodium-Induced	45:58	arg1	Colitis					60:66	Dextran Sulfate Sodium-Induced Colitis	29:66	Dextran Sulfate Sodium-Induced Colitis in Mice	29:74	Indigo Naturalis Ameliorates Dextran Sulfate Sodium-Induced Colitis in Mice by Modulating the Intestinal Microbiota Community.
31726738	3	108	theme	protective	566:575	arg1	action					577:582	the protective action	562:582	the protective action of IN on colitis	562:599	Regarding the significance of alterations of intestinal flora related to IBD and the poor water solubility of the blue IN powder, we predicted that the protective action of IN on colitis may occur through modifying gut microbiota.
31726738	1	109	theme	bowel	292:296	arg1	IBD					307:309	IBD	307:309	IBD	307:309	Indigo naturalis (IN) is a traditional Chinese medicine, named Qing-Dai, which is extracted from indigo plants and has been used to treat patients with inflammatory bowel disease (IBD) in China and Japan.
31726738	1	109	theme	bowel	292:296	arg1	disease					298:304	inflammatory bowel disease	279:304	inflammatory bowel disease (IBD)	279:310	Indigo naturalis (IN) is a traditional Chinese medicine, named Qing-Dai, which is extracted from indigo plants and has been used to treat patients with inflammatory bowel disease (IBD) in China and Japan.
31726738	10	110	theme	lacto	2189:2193	arg1	biosynthesis					2173:2184	glycosphingolipid biosynthesis	2155:2184	glycosphingolipid biosynthesis of lacto and neolacto series	2155:2213	The inferred metagenomes from 16S data using PICRUSt demonstrated that decreased metabolic genes, such as through biosynthesis of siderophore group nonribosomal peptides, non-homologous end-joining, and glycosphingolipid biosynthesis of lacto and neolacto series, may maintain microbiota homeostasis during inflammation from IN treatment in DSS-induced colitis.
31726738	10	110	theme	lacto	2189:2193	arg1	biosynthesis					2066:2077	biosynthesis	2066:2077	biosynthesis of siderophore group nonribosomal peptides	2066:2120	The inferred metagenomes from 16S data using PICRUSt demonstrated that decreased metabolic genes, such as through biosynthesis of siderophore group nonribosomal peptides, non-homologous end-joining, and glycosphingolipid biosynthesis of lacto and neolacto series, may maintain microbiota homeostasis during inflammation from IN treatment in DSS-induced colitis.
31726738	10	110	theme	lacto	2189:2193	arg1	end-joining					2138:2148	non-homologous end-joining	2123:2148	non-homologous end-joining	2123:2148	The inferred metagenomes from 16S data using PICRUSt demonstrated that decreased metabolic genes, such as through biosynthesis of siderophore group nonribosomal peptides, non-homologous end-joining, and glycosphingolipid biosynthesis of lacto and neolacto series, may maintain microbiota homeostasis during inflammation from IN treatment in DSS-induced colitis.
31726738	9	111	theme	Turicibacter	1922:1933	arg1	bacteria					1910:1917	the anaerobic gram-positive bacteria	1882:1917	the anaerobic gram-positive bacteria of Turicibacter	1882:1933	The therapeutic effect of IN may be closely related to the anaerobic gram-positive bacteria of Turicibacter and Peptococcus.
31726738	10	112	from	inflammation	2259:2270	arg1	treatment					2280:2288	treatment	2280:2288	treatment in DSS-induced colitis	2280:2311	The inferred metagenomes from 16S data using PICRUSt demonstrated that decreased metabolic genes, such as through biosynthesis of siderophore group nonribosomal peptides, non-homologous end-joining, and glycosphingolipid biosynthesis of lacto and neolacto series, may maintain microbiota homeostasis during inflammation from IN treatment in DSS-induced colitis.
31726738	4	113	theme	microbiomes	702:712	arg1	relationships					664:676	the relationships	660:676	the relationships of IN, colitis, and gut microbiomes	660:712	To investigate the relationships of IN, colitis, and gut microbiomes, a dextran sulfate sodium (DSS)-induced mice colitis model was tested to explore the protective effects of IN on macroscopic colitis symptoms, the histopathological structure, inflammation cytokines, and gut microbiota, and their potential functions.
31726738	3	114	theme	gut	629:631	arg1	microbiota					633:642	gut microbiota	629:642	gut microbiota	629:642	Regarding the significance of alterations of intestinal flora related to IBD and the poor water solubility of the blue IN powder, we predicted that the protective action of IN on colitis may occur through modifying gut microbiota.
31215863	3	0	theme	chemotaxonomic	295:308	arg1	properties					328:337	chemotaxonomic and morphological properties	295:337	chemotaxonomic and morphological properties consistent with classification in the genus Kribbella	295:391	Strain 16K104T was found to have chemotaxonomic and morphological properties consistent with classification in the genus Kribbella.
31215863	5	1	theme	type	831:834	arg1	strains					836:842	the closely related type strains	811:842	the closely related type strains of the genus	811:855	Multilocus sequence analysis (MLSA) using five housekeeping genes (gyrB, rpoB, relA, recA and atpD) for comparing the strain with all Kribbella type strains showed that the MLSA distances of strain 16K104T to the closely related type strains of the genus were much higher than the 0.04 threshold.
31215863	11	2	theme	Kribbella	1553:1561	arg1	sp					1574:1575	the name Kribbella turkmenica sp	1544:1575	the name Kribbella turkmenica sp	1544:1575	The results of digital DNA-DNA hybridization and average nucleotide identity analyses, in addition to MLSA phylogenetic distances, confirmed that the strain represents a novel species of the genus Kribbella, for which the name Kribbella turkmenica sp.
31215863	11	3	theme	phylogenetic	1433:1444	arg1	distances					1446:1454	MLSA phylogenetic distances	1428:1454	MLSA phylogenetic distances	1428:1454	The results of digital DNA-DNA hybridization and average nucleotide identity analyses, in addition to MLSA phylogenetic distances, confirmed that the strain represents a novel species of the genus Kribbella, for which the name Kribbella turkmenica sp.
31215863	8	4	theme	polar	1090:1094	arg1	diphosphatidylglycerol					1108:1129	diphosphatidylglycerol	1108:1129	diphosphatidylglycerol	1108:1129	The major polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol and phosphatidylinositol.
31215863	8	4	theme	polar	1090:1094	arg1	lipids					1096:1101	The major polar lipids	1080:1101	The major polar lipids	1080:1101	The major polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol and phosphatidylinositol.
31215863	11	5	theme	turkmenica	1563:1572	arg1	sp					1574:1575	the name Kribbella turkmenica sp	1544:1575	the name Kribbella turkmenica sp	1544:1575	The results of digital DNA-DNA hybridization and average nucleotide identity analyses, in addition to MLSA phylogenetic distances, confirmed that the strain represents a novel species of the genus Kribbella, for which the name Kribbella turkmenica sp.
31215863	13	6	theme	=JCM	1624:1627	arg1	49224T					1641:1646	=JCM 32914T=KCTC 49224T	1624:1646	=JCM 32914T=KCTC 49224T	1624:1646	The type strain is 16K104T (=JCM 32914T=KCTC 49224T).
31215863	13	6	theme	=JCM	1624:1627	arg1	16K104T					1615:1621	16K104T	1615:1621	16K104T (=JCM 32914T=KCTC 49224T)	1615:1647	The type strain is 16K104T (=JCM 32914T=KCTC 49224T).
31215863	5	7	theme	related	823:829	arg1	strains					836:842	the closely related type strains	811:842	the closely related type strains of the genus	811:855	Multilocus sequence analysis (MLSA) using five housekeeping genes (gyrB, rpoB, relA, recA and atpD) for comparing the strain with all Kribbella type strains showed that the MLSA distances of strain 16K104T to the closely related type strains of the genus were much higher than the 0.04 threshold.
31215863	4	8	theme	rRNA	573:576	arg1	tree					596:599	the 16S rRNA gene phylogenetic tree	565:599	the 16S rRNA gene phylogenetic tree	565:599	The strain shared the highest 16S rRNA gene sequence similarity with Kribbella albertanoniae BC640T (99.2 %), and formed a branch with Kribbella antibiotica YIM 31530T in the 16S rRNA gene phylogenetic tree.
31215863	8	9	theme	major	1084:1088	arg1	diphosphatidylglycerol					1108:1129	diphosphatidylglycerol	1108:1129	diphosphatidylglycerol	1108:1129	The major polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol and phosphatidylinositol.
31215863	8	9	theme	major	1084:1088	arg1	lipids					1096:1101	The major polar lipids	1080:1101	The major polar lipids	1080:1101	The major polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol and phosphatidylinositol.
31215863	2	10	theme	desert	135:140	arg1	soil					142:145	desert soil	135:145	desert soil collected from the Karakum Desert	135:179	A novel actinobacterial strain, designated 16K104T, was isolated from desert soil collected from the Karakum Desert and characterized using a polyphasic approach to clarify its taxonomic position.
31215863	13	11	theme	32914T=KCTC	1629:1639	arg1	49224T					1641:1646	=JCM 32914T=KCTC 49224T	1624:1646	=JCM 32914T=KCTC 49224T	1624:1646	The type strain is 16K104T (=JCM 32914T=KCTC 49224T).
31215863	13	11	theme	32914T=KCTC	1629:1639	arg1	16K104T					1615:1621	16K104T	1615:1621	16K104T (=JCM 32914T=KCTC 49224T)	1615:1647	The type strain is 16K104T (=JCM 32914T=KCTC 49224T).
31215863	1	12	theme	Karakum	49:55	arg1	Desert					57:62	the Karakum Desert	45:62	the Karakum Desert	45:62	nov., isolated from the Karakum Desert.
31215863	10	13	theme	fatty	1252:1256	arg1	iso-C16 					1269:1276	iso-C16 	1269:1276	iso-C16 	1269:1276	The major fatty acids were iso-C16 : 0, anteiso-C15:0, iso-C15 : 0 and iso-C17 : 0.
31215863	10	13	theme	fatty	1252:1256	arg1	acids					1258:1262	The major fatty acids	1242:1262	The major fatty acids	1242:1262	The major fatty acids were iso-C16 : 0, anteiso-C15:0, iso-C15 : 0 and iso-C17 : 0.
31215863	4	14	theme	16S	569:571	arg1	tree					596:599	the 16S rRNA gene phylogenetic tree	565:599	the 16S rRNA gene phylogenetic tree	565:599	The strain shared the highest 16S rRNA gene sequence similarity with Kribbella albertanoniae BC640T (99.2 %), and formed a branch with Kribbella antibiotica YIM 31530T in the 16S rRNA gene phylogenetic tree.
31215863	3	15	theme	consistent	339:348	arg1	properties					328:337	chemotaxonomic and morphological properties	295:337	chemotaxonomic and morphological properties consistent with classification in the genus Kribbella	295:391	Strain 16K104T was found to have chemotaxonomic and morphological properties consistent with classification in the genus Kribbella.
31215863	5	16	theme	Multilocus	602:611	arg1	analysis					622:629	Multilocus sequence analysis	602:629	Multilocus sequence analysis (MLSA) using five housekeeping genes (gyrB, rpoB, relA, recA and atpD) for comparing the strain with all Kribbella type strains	602:757	Multilocus sequence analysis (MLSA) using five housekeeping genes (gyrB, rpoB, relA, recA and atpD) for comparing the strain with all Kribbella type strains showed that the MLSA distances of strain 16K104T to the closely related type strains of the genus were much higher than the 0.04 threshold.
31215863	5	16	theme	Multilocus	602:611	arg1	MLSA					632:635	MLSA	632:635	MLSA	632:635	Multilocus sequence analysis (MLSA) using five housekeeping genes (gyrB, rpoB, relA, recA and atpD) for comparing the strain with all Kribbella type strains showed that the MLSA distances of strain 16K104T to the closely related type strains of the genus were much higher than the 0.04 threshold.
31215863	6	17	theme	diamino	974:980	arg1	acid					982:985	the diagnostic diamino acid	959:985	the diagnostic diamino acid in the cell-wall peptidoglycan	959:1016	The organism was found to contain ll-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
31215863	6	17	theme	diamino	974:980	arg1	acid					951:954	ll-diaminopimelic acid	933:954	ll-diaminopimelic acid	933:954	The organism was found to contain ll-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
31215863	10	18	theme	major	1246:1250	arg1	iso-C16 					1269:1276	iso-C16 	1269:1276	iso-C16 	1269:1276	The major fatty acids were iso-C16 : 0, anteiso-C15:0, iso-C15 : 0 and iso-C17 : 0.
31215863	10	18	theme	major	1246:1250	arg1	acids					1258:1262	The major fatty acids	1242:1262	The major fatty acids	1242:1262	The major fatty acids were iso-C16 : 0, anteiso-C15:0, iso-C15 : 0 and iso-C17 : 0.
31215863	5	19	theme	strain	793:798	arg1	16K104T					800:806	strain 16K104T	793:806	strain 16K104T	793:806	Multilocus sequence analysis (MLSA) using five housekeeping genes (gyrB, rpoB, relA, recA and atpD) for comparing the strain with all Kribbella type strains showed that the MLSA distances of strain 16K104T to the closely related type strains of the genus were much higher than the 0.04 threshold.
31215863	5	20	dep	genes	662:666	arg1	rpoB					675:678	rpoB	675:678	rpoB	675:678	Multilocus sequence analysis (MLSA) using five housekeeping genes (gyrB, rpoB, relA, recA and atpD) for comparing the strain with all Kribbella type strains showed that the MLSA distances of strain 16K104T to the closely related type strains of the genus were much higher than the 0.04 threshold.
31215863	5	20	dep	genes	662:666	arg1	relA					681:684	relA	681:684	relA	681:684	Multilocus sequence analysis (MLSA) using five housekeeping genes (gyrB, rpoB, relA, recA and atpD) for comparing the strain with all Kribbella type strains showed that the MLSA distances of strain 16K104T to the closely related type strains of the genus were much higher than the 0.04 threshold.
31215863	5	20	dep	genes	662:666	arg1	genes					662:666	five housekeeping genes	644:666	five housekeeping genes (gyrB, rpoB, relA, recA and atpD) for comparing the strain with all Kribbella type strains	644:757	Multilocus sequence analysis (MLSA) using five housekeeping genes (gyrB, rpoB, relA, recA and atpD) for comparing the strain with all Kribbella type strains showed that the MLSA distances of strain 16K104T to the closely related type strains of the genus were much higher than the 0.04 threshold.
31215863	5	20	dep	genes	662:666	arg1	gyrB					669:672	gyrB	669:672	gyrB	669:672	Multilocus sequence analysis (MLSA) using five housekeeping genes (gyrB, rpoB, relA, recA and atpD) for comparing the strain with all Kribbella type strains showed that the MLSA distances of strain 16K104T to the closely related type strains of the genus were much higher than the 0.04 threshold.
31215863	5	20	dep	genes	662:666	arg1	recA					687:690	recA	687:690	recA	687:690	Multilocus sequence analysis (MLSA) using five housekeeping genes (gyrB, rpoB, relA, recA and atpD) for comparing the strain with all Kribbella type strains showed that the MLSA distances of strain 16K104T to the closely related type strains of the genus were much higher than the 0.04 threshold.
31215863	5	20	dep	genes	662:666	arg1	atpD					696:699	atpD	696:699	atpD	696:699	Multilocus sequence analysis (MLSA) using five housekeeping genes (gyrB, rpoB, relA, recA and atpD) for comparing the strain with all Kribbella type strains showed that the MLSA distances of strain 16K104T to the closely related type strains of the genus were much higher than the 0.04 threshold.
31215863	5	21	theme	sequence	613:620	arg1	analysis					622:629	Multilocus sequence analysis	602:629	Multilocus sequence analysis (MLSA) using five housekeeping genes (gyrB, rpoB, relA, recA and atpD) for comparing the strain with all Kribbella type strains	602:757	Multilocus sequence analysis (MLSA) using five housekeeping genes (gyrB, rpoB, relA, recA and atpD) for comparing the strain with all Kribbella type strains showed that the MLSA distances of strain 16K104T to the closely related type strains of the genus were much higher than the 0.04 threshold.
31215863	5	21	theme	sequence	613:620	arg1	MLSA					632:635	MLSA	632:635	MLSA	632:635	Multilocus sequence analysis (MLSA) using five housekeeping genes (gyrB, rpoB, relA, recA and atpD) for comparing the strain with all Kribbella type strains showed that the MLSA distances of strain 16K104T to the closely related type strains of the genus were much higher than the 0.04 threshold.
31215863	3	22	contain	have	290:293	arg1	16K104T					269:275	Strain 16K104T	262:275	Strain 16K104T	262:275	Strain 16K104T was found to have chemotaxonomic and morphological properties consistent with classification in the genus Kribbella.
31215863	3	22	contain	have	290:293	arg2	properties					328:337	chemotaxonomic and morphological properties	295:337	chemotaxonomic and morphological properties consistent with classification in the genus Kribbella	295:391	Strain 16K104T was found to have chemotaxonomic and morphological properties consistent with classification in the genus Kribbella.
31215863	5	23	theme	16K104T	800:806	arg1	distances					780:788	the MLSA distances	771:788	the MLSA distances of strain 16K104T to the closely related type strains of the genus	771:855	Multilocus sequence analysis (MLSA) using five housekeeping genes (gyrB, rpoB, relA, recA and atpD) for comparing the strain with all Kribbella type strains showed that the MLSA distances of strain 16K104T to the closely related type strains of the genus were much higher than the 0.04 threshold.
31215863	5	23	theme	16K104T	800:806	arg1	higher					867:872	higher	867:872	higher	867:872	Multilocus sequence analysis (MLSA) using five housekeeping genes (gyrB, rpoB, relA, recA and atpD) for comparing the strain with all Kribbella type strains showed that the MLSA distances of strain 16K104T to the closely related type strains of the genus were much higher than the 0.04 threshold.
31215863	0	24	theme	turkmenica	10:19	arg1	sp					21:22	Kribbella turkmenica sp	0:22	Kribbella turkmenica sp.	0:23	Kribbella turkmenica sp.
31215863	11	25	dep	distances	1446:1454	arg1	addition					1416:1423	addition	1416:1423	addition	1416:1423	The results of digital DNA-DNA hybridization and average nucleotide identity analyses, in addition to MLSA phylogenetic distances, confirmed that the strain represents a novel species of the genus Kribbella, for which the name Kribbella turkmenica sp.
31215863	11	26	theme	hybridization	1357:1369	arg1	analyses					1403:1410	digital DNA-DNA hybridization and average nucleotide identity analyses	1341:1410	digital DNA-DNA hybridization and average nucleotide identity analyses	1341:1410	The results of digital DNA-DNA hybridization and average nucleotide identity analyses, in addition to MLSA phylogenetic distances, confirmed that the strain represents a novel species of the genus Kribbella, for which the name Kribbella turkmenica sp.
31215863	0	27	theme	Kribbella	0:8	arg1	sp					21:22	Kribbella turkmenica sp	0:22	Kribbella turkmenica sp.	0:23	Kribbella turkmenica sp.
31215863	2	28	theme	Karakum	166:172	arg1	Desert					174:179	the Karakum Desert	162:179	the Karakum Desert	162:179	A novel actinobacterial strain, designated 16K104T, was isolated from desert soil collected from the Karakum Desert and characterized using a polyphasic approach to clarify its taxonomic position.
31215863	6	29	from	acid	982:985	arg1	peptidoglycan					1004:1016	the cell-wall peptidoglycan	990:1016	the cell-wall peptidoglycan	990:1016	The organism was found to contain ll-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
31215863	7	30	theme	whole-cell	1023:1032	arg1	sugars					1034:1039	The whole-cell sugars	1019:1039	The whole-cell sugars	1019:1039	The whole-cell sugars were identified as ribose and glucose.
31215863	7	30	theme	whole-cell	1023:1032	arg1	glucose					1071:1077	glucose	1071:1077	glucose	1071:1077	The whole-cell sugars were identified as ribose and glucose.
31215863	7	30	theme	whole-cell	1023:1032	arg1	ribose					1060:1065	ribose	1060:1065	ribose	1060:1065	The whole-cell sugars were identified as ribose and glucose.
31215863	11	31	theme	analyses	1403:1410	arg1	results					1330:1336	The results	1326:1336	The results of digital DNA-DNA hybridization and average nucleotide identity analyses	1326:1410	The results of digital DNA-DNA hybridization and average nucleotide identity analyses, in addition to MLSA phylogenetic distances, confirmed that the strain represents a novel species of the genus Kribbella, for which the name Kribbella turkmenica sp.
31215863	9	32	theme	predominant	1204:1214	arg1	menaquinone					1216:1226	The predominant menaquinone	1200:1226	The predominant menaquinone	1200:1226	The predominant menaquinone was MK-9(H4).
31215863	9	32	theme	predominant	1204:1214	arg1	MK-9					1232:1235	MK-9	1232:1235	MK-9(H4)	1232:1239	The predominant menaquinone was MK-9(H4).
31215863	3	33	theme	Strain	262:267	arg1	16K104T					269:275	Strain 16K104T	262:275	Strain 16K104T	262:275	Strain 16K104T was found to have chemotaxonomic and morphological properties consistent with classification in the genus Kribbella.
31215863	11	34	theme	novel	1496:1500	arg1	species					1502:1508	a novel species	1494:1508	a novel species	1494:1508	The results of digital DNA-DNA hybridization and average nucleotide identity analyses, in addition to MLSA phylogenetic distances, confirmed that the strain represents a novel species of the genus Kribbella, for which the name Kribbella turkmenica sp.
31215863	3	35	theme	genus	377:381	arg1	Kribbella					383:391	the genus Kribbella	373:391	the genus Kribbella	373:391	Strain 16K104T was found to have chemotaxonomic and morphological properties consistent with classification in the genus Kribbella.
31215863	11	36	theme	DNA-DNA	1349:1355	arg1	hybridization					1357:1369	digital DNA-DNA hybridization	1341:1369	digital DNA-DNA hybridization	1341:1369	The results of digital DNA-DNA hybridization and average nucleotide identity analyses, in addition to MLSA phylogenetic distances, confirmed that the strain represents a novel species of the genus Kribbella, for which the name Kribbella turkmenica sp.
31215863	11	37	theme	nucleotide	1383:1392	arg1	identity					1394:1401	average nucleotide identity	1375:1401	average nucleotide identity	1375:1401	The results of digital DNA-DNA hybridization and average nucleotide identity analyses, in addition to MLSA phylogenetic distances, confirmed that the strain represents a novel species of the genus Kribbella, for which the name Kribbella turkmenica sp.
31215863	5	38	theme	MLSA	775:778	arg1	distances					780:788	the MLSA distances	771:788	the MLSA distances of strain 16K104T to the closely related type strains of the genus	771:855	Multilocus sequence analysis (MLSA) using five housekeeping genes (gyrB, rpoB, relA, recA and atpD) for comparing the strain with all Kribbella type strains showed that the MLSA distances of strain 16K104T to the closely related type strains of the genus were much higher than the 0.04 threshold.
31215863	5	38	theme	MLSA	775:778	arg1	higher					867:872	higher	867:872	higher	867:872	Multilocus sequence analysis (MLSA) using five housekeeping genes (gyrB, rpoB, relA, recA and atpD) for comparing the strain with all Kribbella type strains showed that the MLSA distances of strain 16K104T to the closely related type strains of the genus were much higher than the 0.04 threshold.
31215863	11	39	theme	average	1375:1381	arg1	identity					1394:1401	average nucleotide identity	1375:1401	average nucleotide identity	1375:1401	The results of digital DNA-DNA hybridization and average nucleotide identity analyses, in addition to MLSA phylogenetic distances, confirmed that the strain represents a novel species of the genus Kribbella, for which the name Kribbella turkmenica sp.
31215863	4	40	theme	sequence	438:445	arg1	similarity					447:456	the highest 16S rRNA gene sequence similarity	412:456	the highest 16S rRNA gene sequence similarity	412:456	The strain shared the highest 16S rRNA gene sequence similarity with Kribbella albertanoniae BC640T (99.2 %), and formed a branch with Kribbella antibiotica YIM 31530T in the 16S rRNA gene phylogenetic tree.
31215863	3	41	with	consistent	339:348	arg1	classification					355:368	classification	355:368	classification in the genus Kribbella	355:391	Strain 16K104T was found to have chemotaxonomic and morphological properties consistent with classification in the genus Kribbella.
31215863	11	42	theme	identity	1394:1401	arg1	analyses					1403:1410	digital DNA-DNA hybridization and average nucleotide identity analyses	1341:1410	digital DNA-DNA hybridization and average nucleotide identity analyses	1341:1410	The results of digital DNA-DNA hybridization and average nucleotide identity analyses, in addition to MLSA phylogenetic distances, confirmed that the strain represents a novel species of the genus Kribbella, for which the name Kribbella turkmenica sp.
31215863	6	43	theme	diagnostic	963:972	arg1	acid					982:985	the diagnostic diamino acid	959:985	the diagnostic diamino acid in the cell-wall peptidoglycan	959:1016	The organism was found to contain ll-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
31215863	6	43	theme	diagnostic	963:972	arg1	acid					951:954	ll-diaminopimelic acid	933:954	ll-diaminopimelic acid	933:954	The organism was found to contain ll-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
31215863	3	44	from	classification	355:368	arg1	Kribbella					383:391	the genus Kribbella	373:391	the genus Kribbella	373:391	Strain 16K104T was found to have chemotaxonomic and morphological properties consistent with classification in the genus Kribbella.
31215863	2	45	theme	polyphasic	207:216	arg1	approach					218:225	a polyphasic approach	205:225	a polyphasic approach to clarify its taxonomic position	205:259	A novel actinobacterial strain, designated 16K104T, was isolated from desert soil collected from the Karakum Desert and characterized using a polyphasic approach to clarify its taxonomic position.
31215863	11	46	theme	Kribbella	1523:1531	arg1	species					1502:1508	a novel species	1494:1508	a novel species	1494:1508	The results of digital DNA-DNA hybridization and average nucleotide identity analyses, in addition to MLSA phylogenetic distances, confirmed that the strain represents a novel species of the genus Kribbella, for which the name Kribbella turkmenica sp.
31215863	2	47	theme	actinobacterial	73:87	arg1	strain					89:94	A novel actinobacterial strain	65:94	A novel actinobacterial strain	65:94	A novel actinobacterial strain, designated 16K104T, was isolated from desert soil collected from the Karakum Desert and characterized using a polyphasic approach to clarify its taxonomic position.
31215863	5	48	theme	housekeeping	649:660	arg1	rpoB					675:678	rpoB	675:678	rpoB	675:678	Multilocus sequence analysis (MLSA) using five housekeeping genes (gyrB, rpoB, relA, recA and atpD) for comparing the strain with all Kribbella type strains showed that the MLSA distances of strain 16K104T to the closely related type strains of the genus were much higher than the 0.04 threshold.
31215863	5	48	theme	housekeeping	649:660	arg1	relA					681:684	relA	681:684	relA	681:684	Multilocus sequence analysis (MLSA) using five housekeeping genes (gyrB, rpoB, relA, recA and atpD) for comparing the strain with all Kribbella type strains showed that the MLSA distances of strain 16K104T to the closely related type strains of the genus were much higher than the 0.04 threshold.
31215863	5	48	theme	housekeeping	649:660	arg1	genes					662:666	five housekeeping genes	644:666	five housekeeping genes (gyrB, rpoB, relA, recA and atpD) for comparing the strain with all Kribbella type strains	644:757	Multilocus sequence analysis (MLSA) using five housekeeping genes (gyrB, rpoB, relA, recA and atpD) for comparing the strain with all Kribbella type strains showed that the MLSA distances of strain 16K104T to the closely related type strains of the genus were much higher than the 0.04 threshold.
31215863	5	48	theme	housekeeping	649:660	arg1	gyrB					669:672	gyrB	669:672	gyrB	669:672	Multilocus sequence analysis (MLSA) using five housekeeping genes (gyrB, rpoB, relA, recA and atpD) for comparing the strain with all Kribbella type strains showed that the MLSA distances of strain 16K104T to the closely related type strains of the genus were much higher than the 0.04 threshold.
31215863	5	48	theme	housekeeping	649:660	arg1	recA					687:690	recA	687:690	recA	687:690	Multilocus sequence analysis (MLSA) using five housekeeping genes (gyrB, rpoB, relA, recA and atpD) for comparing the strain with all Kribbella type strains showed that the MLSA distances of strain 16K104T to the closely related type strains of the genus were much higher than the 0.04 threshold.
31215863	5	48	theme	housekeeping	649:660	arg1	atpD					696:699	atpD	696:699	atpD	696:699	Multilocus sequence analysis (MLSA) using five housekeeping genes (gyrB, rpoB, relA, recA and atpD) for comparing the strain with all Kribbella type strains showed that the MLSA distances of strain 16K104T to the closely related type strains of the genus were much higher than the 0.04 threshold.
31215863	5	49	theme	Kribbella	736:744	arg1	strains					751:757	all Kribbella type strains	732:757	all Kribbella type strains	732:757	Multilocus sequence analysis (MLSA) using five housekeeping genes (gyrB, rpoB, relA, recA and atpD) for comparing the strain with all Kribbella type strains showed that the MLSA distances of strain 16K104T to the closely related type strains of the genus were much higher than the 0.04 threshold.
31215863	2	50	theme	novel	67:71	arg1	strain					89:94	A novel actinobacterial strain	65:94	A novel actinobacterial strain	65:94	A novel actinobacterial strain, designated 16K104T, was isolated from desert soil collected from the Karakum Desert and characterized using a polyphasic approach to clarify its taxonomic position.
31215863	4	51	theme	16S	424:426	arg1	similarity					447:456	the highest 16S rRNA gene sequence similarity	412:456	the highest 16S rRNA gene sequence similarity	412:456	The strain shared the highest 16S rRNA gene sequence similarity with Kribbella albertanoniae BC640T (99.2 %), and formed a branch with Kribbella antibiotica YIM 31530T in the 16S rRNA gene phylogenetic tree.
31215863	13	52	theme	type	1600:1603	arg1	strain					1605:1610	The type strain	1596:1610	The type strain	1596:1610	The type strain is 16K104T (=JCM 32914T=KCTC 49224T).
31215863	13	52	theme	type	1600:1603	arg1	16K104T					1615:1621	16K104T	1615:1621	16K104T (=JCM 32914T=KCTC 49224T)	1615:1647	The type strain is 16K104T (=JCM 32914T=KCTC 49224T).
31215863	4	53	theme	highest	416:422	arg1	similarity					447:456	the highest 16S rRNA gene sequence similarity	412:456	the highest 16S rRNA gene sequence similarity	412:456	The strain shared the highest 16S rRNA gene sequence similarity with Kribbella albertanoniae BC640T (99.2 %), and formed a branch with Kribbella antibiotica YIM 31530T in the 16S rRNA gene phylogenetic tree.
31215863	2	54	theme	taxonomic	242:250	arg1	position					252:259	its taxonomic position	238:259	its taxonomic position	238:259	A novel actinobacterial strain, designated 16K104T, was isolated from desert soil collected from the Karakum Desert and characterized using a polyphasic approach to clarify its taxonomic position.
31215863	10	55	dep	iso-C16 	1269:1276	arg1	 0					1322:1323	 0	1322:1323	 0	1322:1323	The major fatty acids were iso-C16 : 0, anteiso-C15:0, iso-C15 : 0 and iso-C17 : 0.
31215863	10	55	dep	iso-C16 	1269:1276	arg1	iso-C17 					1313:1320	iso-C17 	1313:1320	iso-C17 	1313:1320	The major fatty acids were iso-C16 : 0, anteiso-C15:0, iso-C15 : 0 and iso-C17 : 0.
31215863	10	55	dep	iso-C16 	1269:1276	arg1	 0					1306:1307	 0	1306:1307	 0	1306:1307	The major fatty acids were iso-C16 : 0, anteiso-C15:0, iso-C15 : 0 and iso-C17 : 0.
31215863	10	55	dep	iso-C16 	1269:1276	arg1	 0					1278:1279	 0	1278:1279	 0	1278:1279	The major fatty acids were iso-C16 : 0, anteiso-C15:0, iso-C15 : 0 and iso-C17 : 0.
31215863	5	56	theme	genus	851:855	arg1	strains					836:842	the closely related type strains	811:842	the closely related type strains of the genus	811:855	Multilocus sequence analysis (MLSA) using five housekeeping genes (gyrB, rpoB, relA, recA and atpD) for comparing the strain with all Kribbella type strains showed that the MLSA distances of strain 16K104T to the closely related type strains of the genus were much higher than the 0.04 threshold.
31215863	11	57	theme	MLSA	1428:1431	arg1	distances					1446:1454	MLSA phylogenetic distances	1428:1454	MLSA phylogenetic distances	1428:1454	The results of digital DNA-DNA hybridization and average nucleotide identity analyses, in addition to MLSA phylogenetic distances, confirmed that the strain represents a novel species of the genus Kribbella, for which the name Kribbella turkmenica sp.
31215863	4	58	theme	phylogenetic	583:594	arg1	tree					596:599	the 16S rRNA gene phylogenetic tree	565:599	the 16S rRNA gene phylogenetic tree	565:599	The strain shared the highest 16S rRNA gene sequence similarity with Kribbella albertanoniae BC640T (99.2 %), and formed a branch with Kribbella antibiotica YIM 31530T in the 16S rRNA gene phylogenetic tree.
31215863	11	59	theme	genus	1517:1521	arg1	Kribbella					1523:1531	the genus Kribbella	1513:1531	the genus Kribbella	1513:1531	The results of digital DNA-DNA hybridization and average nucleotide identity analyses, in addition to MLSA phylogenetic distances, confirmed that the strain represents a novel species of the genus Kribbella, for which the name Kribbella turkmenica sp.
31215863	4	60	theme	gene	433:436	arg1	similarity					447:456	the highest 16S rRNA gene sequence similarity	412:456	the highest 16S rRNA gene sequence similarity	412:456	The strain shared the highest 16S rRNA gene sequence similarity with Kribbella albertanoniae BC640T (99.2 %), and formed a branch with Kribbella antibiotica YIM 31530T in the 16S rRNA gene phylogenetic tree.
31215863	6	61	contain	contain	925:931	arg1	organism					903:910	The organism	899:910	The organism	899:910	The organism was found to contain ll-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
31215863	6	61	contain	contain	925:931	arg2	acid					951:954	ll-diaminopimelic acid	933:954	ll-diaminopimelic acid	933:954	The organism was found to contain ll-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
31215863	6	61	contain	contain	925:931	arg2	acid					982:985	the diagnostic diamino acid	959:985	the diagnostic diamino acid in the cell-wall peptidoglycan	959:1016	The organism was found to contain ll-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
31215863	2	62	attach	isolated	121:128	arg2	strain					89:94	A novel actinobacterial strain	65:94	A novel actinobacterial strain	65:94	A novel actinobacterial strain, designated 16K104T, was isolated from desert soil collected from the Karakum Desert and characterized using a polyphasic approach to clarify its taxonomic position.
31215863	2	62	attach	isolated	121:128	arg1	soil					142:145	desert soil	135:145	desert soil collected from the Karakum Desert	135:179	A novel actinobacterial strain, designated 16K104T, was isolated from desert soil collected from the Karakum Desert and characterized using a polyphasic approach to clarify its taxonomic position.
31215863	4	63	theme	gene	578:581	arg1	tree					596:599	the 16S rRNA gene phylogenetic tree	565:599	the 16S rRNA gene phylogenetic tree	565:599	The strain shared the highest 16S rRNA gene sequence similarity with Kribbella albertanoniae BC640T (99.2 %), and formed a branch with Kribbella antibiotica YIM 31530T in the 16S rRNA gene phylogenetic tree.
31215863	3	64	theme	morphological	314:326	arg1	properties					328:337	chemotaxonomic and morphological properties	295:337	chemotaxonomic and morphological properties consistent with classification in the genus Kribbella	295:391	Strain 16K104T was found to have chemotaxonomic and morphological properties consistent with classification in the genus Kribbella.
31215863	4	65	theme	rRNA	428:431	arg1	similarity					447:456	the highest 16S rRNA gene sequence similarity	412:456	the highest 16S rRNA gene sequence similarity	412:456	The strain shared the highest 16S rRNA gene sequence similarity with Kribbella albertanoniae BC640T (99.2 %), and formed a branch with Kribbella antibiotica YIM 31530T in the 16S rRNA gene phylogenetic tree.
31215863	11	66	theme	name	1548:1551	arg1	sp					1574:1575	the name Kribbella turkmenica sp	1544:1575	the name Kribbella turkmenica sp	1544:1575	The results of digital DNA-DNA hybridization and average nucleotide identity analyses, in addition to MLSA phylogenetic distances, confirmed that the strain represents a novel species of the genus Kribbella, for which the name Kribbella turkmenica sp.
31215863	6	67	theme	cell-wall	994:1002	arg1	peptidoglycan					1004:1016	the cell-wall peptidoglycan	990:1016	the cell-wall peptidoglycan	990:1016	The organism was found to contain ll-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
31215863	6	68	theme	ll-diaminopimelic	933:949	arg1	acid					982:985	the diagnostic diamino acid	959:985	the diagnostic diamino acid in the cell-wall peptidoglycan	959:1016	The organism was found to contain ll-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
31215863	6	68	theme	ll-diaminopimelic	933:949	arg1	acid					951:954	ll-diaminopimelic acid	933:954	ll-diaminopimelic acid	933:954	The organism was found to contain ll-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
31215863	5	69	theme	type	746:749	arg1	strains					751:757	all Kribbella type strains	732:757	all Kribbella type strains	732:757	Multilocus sequence analysis (MLSA) using five housekeeping genes (gyrB, rpoB, relA, recA and atpD) for comparing the strain with all Kribbella type strains showed that the MLSA distances of strain 16K104T to the closely related type strains of the genus were much higher than the 0.04 threshold.
31215863	11	70	theme	digital	1341:1347	arg1	hybridization					1357:1369	digital DNA-DNA hybridization	1341:1369	digital DNA-DNA hybridization	1341:1369	The results of digital DNA-DNA hybridization and average nucleotide identity analyses, in addition to MLSA phylogenetic distances, confirmed that the strain represents a novel species of the genus Kribbella, for which the name Kribbella turkmenica sp.
29458491	8	0	theme	major	681:685	arg1	acids					702:706	The major cellular fatty acids	677:706	The major cellular fatty acids	677:706	The major cellular fatty acids consisted of iso-C15 : 0, iso-C16 : 0 and anteiso-C15 : 0.
29458491	8	1	theme	fatty	696:700	arg1	acids					702:706	The major cellular fatty acids	677:706	The major cellular fatty acids	677:706	The major cellular fatty acids consisted of iso-C15 : 0, iso-C16 : 0 and anteiso-C15 : 0.
29458491	2	2	dep	WPS1-2T	152:158	arg1	strain					145:150	A novel endophytic actinomycete, strain WPS1-2T	112:158	strain	145:150	A novel endophytic actinomycete, strain WPS1-2T, isolated from a root of Globba winitii C. H. Wright, was characterized taxonomically by using a polyphasic approach.
29458491	10	3	theme	B	943:943	arg1	gene					952:955	the gyrase subunit B (gyrB) gene	924:955	the gyrase subunit B (gyrB) gene	924:955	Phylogenetic analysis of the gyrase subunit B (gyrB) gene indicated that the strain was related to M. costi CS1-12T.
29458491	10	4	theme	costi	1001:1005	arg1	CS1-12T					1007:1013	M. costi CS1-12T	998:1013	M. costi CS1-12T	998:1013	Phylogenetic analysis of the gyrase subunit B (gyrB) gene indicated that the strain was related to M. costi CS1-12T.
29458491	9	5	theme	16S	784:786	arg1	rRNA					788:791	16S rRNA	784:791	the 16S rRNA gene sequence of the strain	780:819	According to the 16S rRNA gene sequence of the strain, WPS1-2T showed highest similarity to Micromonospora costi CS1-12T (99.02 %).
29458491	6	6	contain	contained	568:576	arg2	ribose					587:592	ribose	587:592	ribose	587:592	Whole-cell hydrolysates contained glucose, ribose, arabinose and xylose.
29458491	6	6	contain	contained	568:576	arg2	glucose					578:584	glucose	578:584	glucose	578:584	Whole-cell hydrolysates contained glucose, ribose, arabinose and xylose.
29458491	6	6	contain	contained	568:576	arg1	hydrolysates					555:566	Whole-cell hydrolysates	544:566	Whole-cell hydrolysates	544:566	Whole-cell hydrolysates contained glucose, ribose, arabinose and xylose.
29458491	6	6	contain	contained	568:576	arg2	xylose					609:614	xylose	609:614	xylose	609:614	Whole-cell hydrolysates contained glucose, ribose, arabinose and xylose.
29458491	6	6	contain	contained	568:576	arg2	arabinose					595:603	arabinose	595:603	arabinose	595:603	Whole-cell hydrolysates contained glucose, ribose, arabinose and xylose.
29458491	10	7	theme	gene	952:955	arg1	analysis					912:919	Phylogenetic analysis	899:919	Phylogenetic analysis of the gyrase subunit B (gyrB) gene	899:955	Phylogenetic analysis of the gyrase subunit B (gyrB) gene indicated that the strain was related to M. costi CS1-12T.
29458491	10	8	theme	M.	998:999	arg1	CS1-12T					1007:1013	M. costi CS1-12T	998:1013	M. costi CS1-12T	998:1013	Phylogenetic analysis of the gyrase subunit B (gyrB) gene indicated that the strain was related to M. costi CS1-12T.
29458491	9	9	theme	rRNA	788:791	arg1	sequence					798:805	the 16S rRNA gene sequence	780:805	the 16S rRNA gene sequence of the strain	780:819	According to the 16S rRNA gene sequence of the strain, WPS1-2T showed highest similarity to Micromonospora costi CS1-12T (99.02 %).
29458491	4	10	theme	Single	373:378	arg1	spores					380:385	Single spores	373:385	Single spores	373:385	Single spores were observed directly on substrate mycelia.
29458491	7	11	theme	predominant	621:631	arg1	MK-10					651:655	MK-10	651:655	MK-10	651:655	The predominant menaquinones were MK-10(H8) and MK-10(H10).
29458491	7	11	theme	predominant	621:631	arg1	menaquinones					633:644	The predominant menaquinones	617:644	The predominant menaquinones	617:644	The predominant menaquinones were MK-10(H8) and MK-10(H10).
29458491	6	12	theme	Whole-cell	544:553	arg1	hydrolysates					555:566	Whole-cell hydrolysates	544:566	Whole-cell hydrolysates	544:566	Whole-cell hydrolysates contained glucose, ribose, arabinose and xylose.
29458491	8	13	theme	cellular	687:694	arg1	acids					702:706	The major cellular fatty acids	677:706	The major cellular fatty acids	677:706	The major cellular fatty acids consisted of iso-C15 : 0, iso-C16 : 0 and anteiso-C15 : 0.
29458491	9	14	theme	gene	793:796	arg1	sequence					798:805	the 16S rRNA gene sequence	780:805	the 16S rRNA gene sequence of the strain	780:819	According to the 16S rRNA gene sequence of the strain, WPS1-2T showed highest similarity to Micromonospora costi CS1-12T (99.02 %).
29458491	12	15	theme	morphological	1145:1157	arg1	data					1206:1209	morphological, chemotaxonomic, physiological and biochemical data	1145:1209	morphological, chemotaxonomic, physiological and biochemical data together with DNA-DNA relatedness values	1145:1250	The strain could be distinguished from closely related type strains by using a combination of morphological, chemotaxonomic, physiological and biochemical data together with DNA-DNA relatedness values.
29458491	5	16	theme	meso-diaminopimelic	484:502	arg1	acid					504:507	meso-diaminopimelic acid	484:507	meso-diaminopimelic acid	484:507	The cell-wall peptidoglycan of the strain contained meso-diaminopimelic acid and 3-OH-meso-diaminopimelic acid.
29458491	13	17	theme	Micromonospora	1388:1401	arg1	sp					1411:1412	the name Micromonospora globbae sp	1379:1412	the name Micromonospora globbae sp	1379:1412	Based on these observations, strain WPS1-2T is considered to represent a novel species of the genus Micromonospora, for which the name Micromonospora globbae sp.
29458491	12	18	theme	type	1106:1109	arg1	strains					1111:1117	closely related type strains	1090:1117	closely related type strains	1090:1117	The strain could be distinguished from closely related type strains by using a combination of morphological, chemotaxonomic, physiological and biochemical data together with DNA-DNA relatedness values.
29458491	2	19	theme	actinomycete	131:142	arg1	WPS1-2T					152:158	A novel endophytic actinomycete, strain WPS1-2T	112:158	WPS1-2T	152:158	A novel endophytic actinomycete, strain WPS1-2T, isolated from a root of Globba winitii C. H. Wright, was characterized taxonomically by using a polyphasic approach.
29458491	2	20	dep	Globba	185:190	arg1	winitii					192:198	Globba winitii C. H. Wright	185:211	Globba winitii C. H. Wright	185:211	A novel endophytic actinomycete, strain WPS1-2T, isolated from a root of Globba winitii C. H. Wright, was characterized taxonomically by using a polyphasic approach.
29458491	2	20	dep	Globba	185:190	arg1	Wright					206:211	Wright	206:211	Wright	206:211	A novel endophytic actinomycete, strain WPS1-2T, isolated from a root of Globba winitii C. H. Wright, was characterized taxonomically by using a polyphasic approach.
29458491	12	21	theme	relatedness	1233:1243	arg1	values					1245:1250	DNA-DNA relatedness values	1225:1250	DNA-DNA relatedness values	1225:1250	The strain could be distinguished from closely related type strains by using a combination of morphological, chemotaxonomic, physiological and biochemical data together with DNA-DNA relatedness values.
29458491	12	22	theme	related	1098:1104	arg1	strains					1111:1117	closely related type strains	1090:1117	closely related type strains	1090:1117	The strain could be distinguished from closely related type strains by using a combination of morphological, chemotaxonomic, physiological and biochemical data together with DNA-DNA relatedness values.
29458491	2	23	theme	endophytic	120:129	arg1	WPS1-2T					152:158	A novel endophytic actinomycete, strain WPS1-2T	112:158	WPS1-2T	152:158	A novel endophytic actinomycete, strain WPS1-2T, isolated from a root of Globba winitii C. H. Wright, was characterized taxonomically by using a polyphasic approach.
29458491	12	24	theme	DNA-DNA	1225:1231	arg1	values					1245:1250	DNA-DNA relatedness values	1225:1250	DNA-DNA relatedness values	1225:1250	The strain could be distinguished from closely related type strains by using a combination of morphological, chemotaxonomic, physiological and biochemical data together with DNA-DNA relatedness values.
29458491	0	25	theme	globbae	15:21	arg1	sp					23:24	Micromonospora globbae sp	0:24	Micromonospora globbae sp.	0:25	Micromonospora globbae sp.
29458491	2	26	theme	novel	114:118	arg1	WPS1-2T					152:158	A novel endophytic actinomycete, strain WPS1-2T	112:158	WPS1-2T	152:158	A novel endophytic actinomycete, strain WPS1-2T, isolated from a root of Globba winitii C. H. Wright, was characterized taxonomically by using a polyphasic approach.
29458491	13	27	theme	novel	1326:1330	arg1	species					1332:1338	a novel species	1324:1338	a novel species	1324:1338	Based on these observations, strain WPS1-2T is considered to represent a novel species of the genus Micromonospora, for which the name Micromonospora globbae sp.
29458491	5	28	contain	contained	474:482	arg2	acid					538:541	3-OH-meso-diaminopimelic acid	513:541	3-OH-meso-diaminopimelic acid	513:541	The cell-wall peptidoglycan of the strain contained meso-diaminopimelic acid and 3-OH-meso-diaminopimelic acid.
29458491	5	28	contain	contained	474:482	arg2	acid					504:507	meso-diaminopimelic acid	484:507	meso-diaminopimelic acid	484:507	The cell-wall peptidoglycan of the strain contained meso-diaminopimelic acid and 3-OH-meso-diaminopimelic acid.
29458491	5	28	contain	contained	474:482	arg1	peptidoglycan					446:458	The cell-wall peptidoglycan	432:458	The cell-wall peptidoglycan of the strain	432:472	The cell-wall peptidoglycan of the strain contained meso-diaminopimelic acid and 3-OH-meso-diaminopimelic acid.
29458491	5	29	theme	3-OH-meso-diaminopimelic	513:536	arg1	acid					538:541	3-OH-meso-diaminopimelic acid	513:541	3-OH-meso-diaminopimelic acid	513:541	The cell-wall peptidoglycan of the strain contained meso-diaminopimelic acid and 3-OH-meso-diaminopimelic acid.
29458491	0	30	theme	Micromonospora	0:13	arg1	sp					23:24	Micromonospora globbae sp	0:24	Micromonospora globbae sp.	0:25	Micromonospora globbae sp.
29458491	1	31	attach	isolated	60:67	arg2	actinomycete					47:58	an endophytic actinomycete	33:58	an endophytic actinomycete isolated from roots of Globba winitii C. H. Wright	33:109	nov., an endophytic actinomycete isolated from roots of Globba winitii C. H. Wright.
29458491	1	31	attach	isolated	60:67	arg1	roots					74:78	roots	74:78	roots of Globba winitii C. H. Wright	74:109	nov., an endophytic actinomycete isolated from roots of Globba winitii C. H. Wright.
29458491	8	32	dep	consisted	708:716	arg1	 0					730:731	 0	730:731	 0	730:731	The major cellular fatty acids consisted of iso-C15 : 0, iso-C16 : 0 and anteiso-C15 : 0.
29458491	10	33	theme	Phylogenetic	899:910	arg1	analysis					912:919	Phylogenetic analysis	899:919	Phylogenetic analysis of the gyrase subunit B (gyrB) gene	899:955	Phylogenetic analysis of the gyrase subunit B (gyrB) gene indicated that the strain was related to M. costi CS1-12T.
29458491	12	34	theme	data	1206:1209	arg1	combination					1130:1140	a combination	1128:1140	a combination of morphological, chemotaxonomic, physiological and biochemical data together with DNA-DNA relatedness values	1128:1250	The strain could be distinguished from closely related type strains by using a combination of morphological, chemotaxonomic, physiological and biochemical data together with DNA-DNA relatedness values.
29458491	4	35	located	observed	392:399	arg2	spores					380:385	Single spores	373:385	Single spores	373:385	Single spores were observed directly on substrate mycelia.
29458491	4	35	located	observed	392:399	arg1	mycelia					423:429	substrate mycelia	413:429	substrate mycelia	413:429	Single spores were observed directly on substrate mycelia.
29458491	15	36	theme	39787T=NBRC	1467:1477	arg1	2405T					1493:1497	=KCTC 39787T=NBRC 112325T=TISTR 2405T	1461:1497	=KCTC 39787T=NBRC 112325T=TISTR 2405T	1461:1497	The type strain is WPS1-2T (=KCTC 39787T=NBRC 112325T=TISTR 2405T).
29458491	15	36	theme	39787T=NBRC	1467:1477	arg1	WPS1-2T					1452:1458	WPS1-2T	1452:1458	WPS1-2T (=KCTC 39787T=NBRC 112325T=TISTR 2405T)	1452:1498	The type strain is WPS1-2T (=KCTC 39787T=NBRC 112325T=TISTR 2405T).
29458491	2	37	theme	polyphasic	257:266	arg1	approach					268:275	a polyphasic approach	255:275	a polyphasic approach	255:275	A novel endophytic actinomycete, strain WPS1-2T, isolated from a root of Globba winitii C. H. Wright, was characterized taxonomically by using a polyphasic approach.
29458491	5	38	theme	strain	467:472	arg1	peptidoglycan					446:458	The cell-wall peptidoglycan	432:458	The cell-wall peptidoglycan of the strain	432:472	The cell-wall peptidoglycan of the strain contained meso-diaminopimelic acid and 3-OH-meso-diaminopimelic acid.
29458491	2	39	attach	isolated	161:168	arg1	root					177:180	a root	175:180	a root of Globba winitii C. H. Wright	175:211	A novel endophytic actinomycete, strain WPS1-2T, isolated from a root of Globba winitii C. H. Wright, was characterized taxonomically by using a polyphasic approach.
29458491	2	39	attach	isolated	161:168	arg2	WPS1-2T					152:158	A novel endophytic actinomycete, strain WPS1-2T	112:158	WPS1-2T	152:158	A novel endophytic actinomycete, strain WPS1-2T, isolated from a root of Globba winitii C. H. Wright, was characterized taxonomically by using a polyphasic approach.
29458491	12	40	theme	chemotaxonomic	1160:1173	arg1	data					1206:1209	morphological, chemotaxonomic, physiological and biochemical data	1145:1209	morphological, chemotaxonomic, physiological and biochemical data together with DNA-DNA relatedness values	1145:1250	The strain could be distinguished from closely related type strains by using a combination of morphological, chemotaxonomic, physiological and biochemical data together with DNA-DNA relatedness values.
29458491	15	41	theme	112325T=TISTR	1479:1491	arg1	2405T					1493:1497	=KCTC 39787T=NBRC 112325T=TISTR 2405T	1461:1497	=KCTC 39787T=NBRC 112325T=TISTR 2405T	1461:1497	The type strain is WPS1-2T (=KCTC 39787T=NBRC 112325T=TISTR 2405T).
29458491	15	41	theme	112325T=TISTR	1479:1491	arg1	WPS1-2T					1452:1458	WPS1-2T	1452:1458	WPS1-2T (=KCTC 39787T=NBRC 112325T=TISTR 2405T)	1452:1498	The type strain is WPS1-2T (=KCTC 39787T=NBRC 112325T=TISTR 2405T).
29458491	10	42	theme	subunit	935:941	arg1	gene					952:955	the gyrase subunit B (gyrB) gene	924:955	the gyrase subunit B (gyrB) gene	924:955	Phylogenetic analysis of the gyrase subunit B (gyrB) gene indicated that the strain was related to M. costi CS1-12T.
29458491	9	43	theme	strain	814:819	arg1	sequence					798:805	the 16S rRNA gene sequence	780:805	the 16S rRNA gene sequence of the strain	780:819	According to the 16S rRNA gene sequence of the strain, WPS1-2T showed highest similarity to Micromonospora costi CS1-12T (99.02 %).
29458491	9	44	theme	highest	837:843	arg1	similarity					845:854	highest similarity	837:854	highest similarity to Micromonospora costi CS1-12T (99.02 %)	837:896	According to the 16S rRNA gene sequence of the strain, WPS1-2T showed highest similarity to Micromonospora costi CS1-12T (99.02 %).
29458491	10	45	theme	gyrase	928:933	arg1	gene					952:955	the gyrase subunit B (gyrB) gene	924:955	the gyrase subunit B (gyrB) gene	924:955	Phylogenetic analysis of the gyrase subunit B (gyrB) gene indicated that the strain was related to M. costi CS1-12T.
29458491	11	46	theme	DNA	1020:1022	arg1	G+C content					1024:1034	The DNA G+C content	1016:1034	The DNA G+C content	1016:1034	The DNA G+C content was 73.7 mol%.
29458491	11	46	theme	DNA	1020:1022	arg1	%					1048:1048	73.7 mol%	1040:1048	73.7 mol%	1040:1048	The DNA G+C content was 73.7 mol%.
29458491	8	47	dep	 0	730:731	arg1	anteiso-C15 					750:761	anteiso-C15 	750:761	anteiso-C15 	750:761	The major cellular fatty acids consisted of iso-C15 : 0, iso-C16 : 0 and anteiso-C15 : 0.
29458491	8	47	dep	 0	730:731	arg1	 0					743:744	 0	743:744	 0	743:744	The major cellular fatty acids consisted of iso-C15 : 0, iso-C16 : 0 and anteiso-C15 : 0.
29458491	8	47	dep	 0	730:731	arg1	 0					763:764	 0	763:764	 0	763:764	The major cellular fatty acids consisted of iso-C15 : 0, iso-C16 : 0 and anteiso-C15 : 0.
29458491	11	48	theme	73.7 mol	1040:1047	arg1	G+C content					1024:1034	The DNA G+C content	1016:1034	The DNA G+C content	1016:1034	The DNA G+C content was 73.7 mol%.
29458491	11	48	theme	73.7 mol	1040:1047	arg1	%					1048:1048	73.7 mol%	1040:1048	73.7 mol%	1040:1048	The DNA G+C content was 73.7 mol%.
29458491	13	49	theme	name	1383:1386	arg1	sp					1411:1412	the name Micromonospora globbae sp	1379:1412	the name Micromonospora globbae sp	1379:1412	Based on these observations, strain WPS1-2T is considered to represent a novel species of the genus Micromonospora, for which the name Micromonospora globbae sp.
29458491	3	50	theme	Strain	278:283	arg1	WPS1-2T					285:291	Strain WPS1-2T	278:291	Strain WPS1-2T	278:291	Strain WPS1-2T exhibited identical characteristics to the members of the genus Micromonospora.
29458491	2	51	theme	Globba	185:190	arg1	root					177:180	a root	175:180	a root of Globba winitii C. H. Wright	175:211	A novel endophytic actinomycete, strain WPS1-2T, isolated from a root of Globba winitii C. H. Wright, was characterized taxonomically by using a polyphasic approach.
29458491	1	52	theme	Wright	104:109	arg1	roots					74:78	roots	74:78	roots of Globba winitii C. H. Wright	74:109	nov., an endophytic actinomycete isolated from roots of Globba winitii C. H. Wright.
29458491	12	53	theme	biochemical	1194:1204	arg1	data					1206:1209	morphological, chemotaxonomic, physiological and biochemical data	1145:1209	morphological, chemotaxonomic, physiological and biochemical data together with DNA-DNA relatedness values	1145:1250	The strain could be distinguished from closely related type strains by using a combination of morphological, chemotaxonomic, physiological and biochemical data together with DNA-DNA relatedness values.
29458491	1	54	theme	endophytic	36:45	arg1	actinomycete					47:58	an endophytic actinomycete	33:58	an endophytic actinomycete isolated from roots of Globba winitii C. H. Wright	33:109	nov., an endophytic actinomycete isolated from roots of Globba winitii C. H. Wright.
29458491	9	55	theme	Micromonospora	859:872	arg1	CS1-12T					880:886	Micromonospora costi CS1-12T	859:886	Micromonospora costi CS1-12T (99.02 %)	859:896	According to the 16S rRNA gene sequence of the strain, WPS1-2T showed highest similarity to Micromonospora costi CS1-12T (99.02 %).
29458491	9	55	theme	Micromonospora	859:872	arg1	%					895:895	99.02 %	889:895	99.02 %	889:895	According to the 16S rRNA gene sequence of the strain, WPS1-2T showed highest similarity to Micromonospora costi CS1-12T (99.02 %).
29458491	1	56	dep	actinomycete	47:58	arg1	nov.					27:30	nov.	27:30	nov.	27:30	nov., an endophytic actinomycete isolated from roots of Globba winitii C. H. Wright.
29458491	9	57	theme	costi	874:878	arg1	CS1-12T					880:886	Micromonospora costi CS1-12T	859:886	Micromonospora costi CS1-12T (99.02 %)	859:896	According to the 16S rRNA gene sequence of the strain, WPS1-2T showed highest similarity to Micromonospora costi CS1-12T (99.02 %).
29458491	9	57	theme	costi	874:878	arg1	%					895:895	99.02 %	889:895	99.02 %	889:895	According to the 16S rRNA gene sequence of the strain, WPS1-2T showed highest similarity to Micromonospora costi CS1-12T (99.02 %).
29458491	15	58	theme	type	1437:1440	arg1	WPS1-2T					1452:1458	WPS1-2T	1452:1458	WPS1-2T (=KCTC 39787T=NBRC 112325T=TISTR 2405T)	1452:1498	The type strain is WPS1-2T (=KCTC 39787T=NBRC 112325T=TISTR 2405T).
29458491	15	58	theme	type	1437:1440	arg1	strain					1442:1447	The type strain	1433:1447	The type strain	1433:1447	The type strain is WPS1-2T (=KCTC 39787T=NBRC 112325T=TISTR 2405T).
29458491	10	59	theme	gyrB	946:949	arg1	gene					952:955	the gyrase subunit B (gyrB) gene	924:955	the gyrase subunit B (gyrB) gene	924:955	Phylogenetic analysis of the gyrase subunit B (gyrB) gene indicated that the strain was related to M. costi CS1-12T.
29458491	15	60	theme	=KCTC	1461:1465	arg1	2405T					1493:1497	=KCTC 39787T=NBRC 112325T=TISTR 2405T	1461:1497	=KCTC 39787T=NBRC 112325T=TISTR 2405T	1461:1497	The type strain is WPS1-2T (=KCTC 39787T=NBRC 112325T=TISTR 2405T).
29458491	15	60	theme	=KCTC	1461:1465	arg1	WPS1-2T					1452:1458	WPS1-2T	1452:1458	WPS1-2T (=KCTC 39787T=NBRC 112325T=TISTR 2405T)	1452:1498	The type strain is WPS1-2T (=KCTC 39787T=NBRC 112325T=TISTR 2405T).
29458491	12	61	theme	physiological	1176:1188	arg1	data					1206:1209	morphological, chemotaxonomic, physiological and biochemical data	1145:1209	morphological, chemotaxonomic, physiological and biochemical data together with DNA-DNA relatedness values	1145:1250	The strain could be distinguished from closely related type strains by using a combination of morphological, chemotaxonomic, physiological and biochemical data together with DNA-DNA relatedness values.
29458491	5	62	theme	cell-wall	436:444	arg1	peptidoglycan					446:458	The cell-wall peptidoglycan	432:458	The cell-wall peptidoglycan of the strain	432:472	The cell-wall peptidoglycan of the strain contained meso-diaminopimelic acid and 3-OH-meso-diaminopimelic acid.
29458491	13	63	theme	strain	1282:1287	arg1	WPS1-2T					1289:1295	strain WPS1-2T	1282:1295	strain WPS1-2T	1282:1295	Based on these observations, strain WPS1-2T is considered to represent a novel species of the genus Micromonospora, for which the name Micromonospora globbae sp.
29458491	3	64	theme	identical	303:311	arg1	characteristics					313:327	identical characteristics	303:327	identical characteristics	303:327	Strain WPS1-2T exhibited identical characteristics to the members of the genus Micromonospora.
29458491	4	65	theme	substrate	413:421	arg1	mycelia					423:429	substrate mycelia	413:429	substrate mycelia	413:429	Single spores were observed directly on substrate mycelia.
29458491	13	66	theme	globbae	1403:1409	arg1	sp					1411:1412	the name Micromonospora globbae sp	1379:1412	the name Micromonospora globbae sp	1379:1412	Based on these observations, strain WPS1-2T is considered to represent a novel species of the genus Micromonospora, for which the name Micromonospora globbae sp.
29458491	13	67	theme	genus	1347:1351	arg1	Micromonospora					1353:1366	the genus Micromonospora	1343:1366	the genus Micromonospora	1343:1366	Based on these observations, strain WPS1-2T is considered to represent a novel species of the genus Micromonospora, for which the name Micromonospora globbae sp.
29458491	13	68	theme	Micromonospora	1353:1366	arg1	species					1332:1338	a novel species	1324:1338	a novel species	1324:1338	Based on these observations, strain WPS1-2T is considered to represent a novel species of the genus Micromonospora, for which the name Micromonospora globbae sp.
29458491	3	69	theme	genus	351:355	arg1	Micromonospora					357:370	the genus Micromonospora	347:370	the genus Micromonospora	347:370	Strain WPS1-2T exhibited identical characteristics to the members of the genus Micromonospora.
29458491	3	70	theme	Micromonospora	357:370	arg1	members					336:342	the members	332:342	the members of the genus Micromonospora	332:370	Strain WPS1-2T exhibited identical characteristics to the members of the genus Micromonospora.
29393579	7	0	theme	xylan	1102:1106	arg1	genes					1121:1125	xylan biosynthetic genes	1102:1125	the xylan biosynthetic genes IRX9, IRX10, and FRA8	1098:1147	The knat7 loss-of-function mutants had lower transcript levels of the xylan biosynthetic genes IRX9, IRX10, and FRA8, whereas KNAT7 overexpression plants had higher mRNA levels for IRX9, IRX10, IRX14L, and FRA8.
29393579	7	0	theme	xylan	1102:1106	arg1	IRX10					1133:1137	IRX10	1133:1137	IRX10	1133:1137	The knat7 loss-of-function mutants had lower transcript levels of the xylan biosynthetic genes IRX9, IRX10, and FRA8, whereas KNAT7 overexpression plants had higher mRNA levels for IRX9, IRX10, IRX14L, and FRA8.
29393579	7	0	theme	xylan	1102:1106	arg1	FRA8					1144:1147	FRA8	1144:1147	FRA8	1144:1147	The knat7 loss-of-function mutants had lower transcript levels of the xylan biosynthetic genes IRX9, IRX10, and FRA8, whereas KNAT7 overexpression plants had higher mRNA levels for IRX9, IRX10, IRX14L, and FRA8.
29393579	7	0	theme	xylan	1102:1106	arg1	IRX9					1127:1130	IRX9	1127:1130	IRX9	1127:1130	The knat7 loss-of-function mutants had lower transcript levels of the xylan biosynthetic genes IRX9, IRX10, and FRA8, whereas KNAT7 overexpression plants had higher mRNA levels for IRX9, IRX10, IRX14L, and FRA8.
29393579	7	1	contain	had	1186:1188	arg2	levels					1202:1207	higher mRNA levels	1190:1207	higher mRNA levels for IRX9, IRX10, IRX14L, and FRA8	1190:1241	The knat7 loss-of-function mutants had lower transcript levels of the xylan biosynthetic genes IRX9, IRX10, and FRA8, whereas KNAT7 overexpression plants had higher mRNA levels for IRX9, IRX10, IRX14L, and FRA8.
29393579	7	1	contain	had	1186:1188	arg1	plants					1179:1184	KNAT7 overexpression plants	1158:1184	KNAT7 overexpression plants	1158:1184	The knat7 loss-of-function mutants had lower transcript levels of the xylan biosynthetic genes IRX9, IRX10, and FRA8, whereas KNAT7 overexpression plants had higher mRNA levels for IRX9, IRX10, IRX14L, and FRA8.
29393579	1	2	theme	secondary	163:171	arg1	wall					178:181	the secondary cell wall	159:181	the secondary cell wall	159:181	Xylan is the major plant hemicellulosic polysaccharide in the secondary cell wall.
29393579	6	3	from	levels	892:897	arg1	stems					922:926	knat7 inflorescence stems	902:926	knat7 inflorescence stems	902:926	Glycosyl linkage and sugar composition analyses revealed lower xylan levels in knat7 inflorescence stems, relative to WT; a finding supported by labeling of inflorescence walls with xylan-specific antibodies.
29393579	6	4	theme	xylan	886:890	arg1	levels					892:897	lower xylan levels	880:897	lower xylan levels	880:897	Glycosyl linkage and sugar composition analyses revealed lower xylan levels in knat7 inflorescence stems, relative to WT; a finding supported by labeling of inflorescence walls with xylan-specific antibodies.
29393579	7	5	theme	biosynthetic	1108:1119	arg1	genes					1121:1125	xylan biosynthetic genes	1102:1125	the xylan biosynthetic genes IRX9, IRX10, and FRA8	1098:1147	The knat7 loss-of-function mutants had lower transcript levels of the xylan biosynthetic genes IRX9, IRX10, and FRA8, whereas KNAT7 overexpression plants had higher mRNA levels for IRX9, IRX10, IRX14L, and FRA8.
29393579	7	5	theme	biosynthetic	1108:1119	arg1	IRX10					1133:1137	IRX10	1133:1137	IRX10	1133:1137	The knat7 loss-of-function mutants had lower transcript levels of the xylan biosynthetic genes IRX9, IRX10, and FRA8, whereas KNAT7 overexpression plants had higher mRNA levels for IRX9, IRX10, IRX14L, and FRA8.
29393579	7	5	theme	biosynthetic	1108:1119	arg1	FRA8					1144:1147	FRA8	1144:1147	FRA8	1144:1147	The knat7 loss-of-function mutants had lower transcript levels of the xylan biosynthetic genes IRX9, IRX10, and FRA8, whereas KNAT7 overexpression plants had higher mRNA levels for IRX9, IRX10, IRX14L, and FRA8.
29393579	7	5	theme	biosynthetic	1108:1119	arg1	IRX9					1127:1130	IRX9	1127:1130	IRX9	1127:1130	The knat7 loss-of-function mutants had lower transcript levels of the xylan biosynthetic genes IRX9, IRX10, and FRA8, whereas KNAT7 overexpression plants had higher mRNA levels for IRX9, IRX10, IRX14L, and FRA8.
29393579	7	6	theme	lower	1071:1075	arg1	levels					1088:1093	lower transcript levels	1071:1093	lower transcript levels of the xylan biosynthetic genes IRX9, IRX10, and FRA8	1071:1147	The knat7 loss-of-function mutants had lower transcript levels of the xylan biosynthetic genes IRX9, IRX10, and FRA8, whereas KNAT7 overexpression plants had higher mRNA levels for IRX9, IRX10, IRX14L, and FRA8.
29393579	1	7	theme	cell	173:176	arg1	wall					178:181	the secondary cell wall	159:181	the secondary cell wall	159:181	Xylan is the major plant hemicellulosic polysaccharide in the secondary cell wall.
29393579	4	8	from	walls	650:654	arg1	stems					732:736	inflorescence stems	718:736	inflorescence stems	718:736	The knat7 T-DNA insertion mutants have thinner vessel element walls and xylary fibers, and thicker interfascicular fiber walls in inflorescence stems, relative to wild-type (WT).
29393579	6	9	with	labeling	968:975	arg1	antibodies					1020:1029	xylan-specific antibodies	1005:1029	xylan-specific antibodies	1005:1029	Glycosyl linkage and sugar composition analyses revealed lower xylan levels in knat7 inflorescence stems, relative to WT; a finding supported by labeling of inflorescence walls with xylan-specific antibodies.
29393579	4	10	from	walls	709:713	arg1	stems					732:736	inflorescence stems	718:736	inflorescence stems	718:736	The knat7 T-DNA insertion mutants have thinner vessel element walls and xylary fibers, and thicker interfascicular fiber walls in inflorescence stems, relative to wild-type (WT).
29393579	3	11	theme	XYLEM	536:540	arg1	IRX14L					551:556	IRX14L	551:556	IRX14L	551:556	Using transactivation analyses, we demonstrate that KNAT7 activates the promoters of the xylan biosynthetic genes, IRREGULAR XYLEM 9 (IRX9), IRX10, IRREGULAR XYLEM 14-LIKE (IRX14L), and FRAGILE FIBER 8 (FRA8).
29393579	3	11	theme	XYLEM	536:540	arg1	14-LIKE					542:548	IRREGULAR XYLEM 14-LIKE	526:548	IRREGULAR XYLEM 14-LIKE (IRX14L)	526:557	Using transactivation analyses, we demonstrate that KNAT7 activates the promoters of the xylan biosynthetic genes, IRREGULAR XYLEM 9 (IRX9), IRX10, IRREGULAR XYLEM 14-LIKE (IRX14L), and FRAGILE FIBER 8 (FRA8).
29393579	3	11	theme	XYLEM	536:540	arg1	genes					486:490	the xylan biosynthetic genes	463:490	the xylan biosynthetic genes	463:490	Using transactivation analyses, we demonstrate that KNAT7 activates the promoters of the xylan biosynthetic genes, IRREGULAR XYLEM 9 (IRX9), IRX10, IRREGULAR XYLEM 14-LIKE (IRX14L), and FRAGILE FIBER 8 (FRA8).
29393579	4	12	theme	inflorescence	718:730	arg1	stems					732:736	inflorescence stems	718:736	inflorescence stems	718:736	The knat7 T-DNA insertion mutants have thinner vessel element walls and xylary fibers, and thicker interfascicular fiber walls in inflorescence stems, relative to wild-type (WT).
29393579	4	13	theme	xylary	660:665	arg1	fibers					667:672	xylary fibers	660:672	xylary fibers	660:672	The knat7 T-DNA insertion mutants have thinner vessel element walls and xylary fibers, and thicker interfascicular fiber walls in inflorescence stems, relative to wild-type (WT).
29393579	5	14	theme	opposite	805:812	arg1	effects					814:820	opposite effects	805:820	opposite effects	805:820	KNAT7 overexpression plants exhibited opposite effects.
29393579	7	15	theme	transcript	1077:1086	arg1	levels					1088:1093	lower transcript levels	1071:1093	lower transcript levels of the xylan biosynthetic genes IRX9, IRX10, and FRA8	1071:1147	The knat7 loss-of-function mutants had lower transcript levels of the xylan biosynthetic genes IRX9, IRX10, and FRA8, whereas KNAT7 overexpression plants had higher mRNA levels for IRX9, IRX10, IRX14L, and FRA8.
29393579	2	16	theme	wall	290:293	arg1	biosynthesis					295:306	secondary cell wall biosynthesis	275:306	secondary cell wall biosynthesis	275:306	The transcription factor KNOTTED-LIKE HOMEOBOX OF ARABIDOPSIS THALIANA 7 (KNAT7) regulates secondary cell wall biosynthesis, but its exact role in regulating xylan biosynthesis remains unclear.
29393579	4	17	dep	wild-type	751:759	arg1	WT					762:763	WT	762:763	WT	762:763	The knat7 T-DNA insertion mutants have thinner vessel element walls and xylary fibers, and thicker interfascicular fiber walls in inflorescence stems, relative to wild-type (WT).
29393579	6	18	theme	Glycosyl	823:830	arg1	linkage					832:838	Glycosyl linkage	823:838	Glycosyl linkage	823:838	Glycosyl linkage and sugar composition analyses revealed lower xylan levels in knat7 inflorescence stems, relative to WT; a finding supported by labeling of inflorescence walls with xylan-specific antibodies.
29393579	7	19	theme	KNAT7	1158:1162	arg1	plants					1179:1184	KNAT7 overexpression plants	1158:1184	KNAT7 overexpression plants	1158:1184	The knat7 loss-of-function mutants had lower transcript levels of the xylan biosynthetic genes IRX9, IRX10, and FRA8, whereas KNAT7 overexpression plants had higher mRNA levels for IRX9, IRX10, IRX14L, and FRA8.
29393579	6	20	theme	composition	850:860	arg1	analyses					862:869	Glycosyl linkage and sugar composition analyses	823:869	Glycosyl linkage and sugar composition analyses	823:869	Glycosyl linkage and sugar composition analyses revealed lower xylan levels in knat7 inflorescence stems, relative to WT; a finding supported by labeling of inflorescence walls with xylan-specific antibodies.
29393579	2	21	theme	cell	285:288	arg1	biosynthesis					295:306	secondary cell wall biosynthesis	275:306	secondary cell wall biosynthesis	275:306	The transcription factor KNOTTED-LIKE HOMEOBOX OF ARABIDOPSIS THALIANA 7 (KNAT7) regulates secondary cell wall biosynthesis, but its exact role in regulating xylan biosynthesis remains unclear.
29393579	6	22	theme	relative	929:936	arg1	levels					892:897	lower xylan levels	880:897	lower xylan levels	880:897	Glycosyl linkage and sugar composition analyses revealed lower xylan levels in knat7 inflorescence stems, relative to WT; a finding supported by labeling of inflorescence walls with xylan-specific antibodies.
29393579	6	23	theme	sugar	844:848	arg1	composition					850:860	sugar composition	844:860	sugar composition	844:860	Glycosyl linkage and sugar composition analyses revealed lower xylan levels in knat7 inflorescence stems, relative to WT; a finding supported by labeling of inflorescence walls with xylan-specific antibodies.
29393579	7	24	theme	higher	1190:1195	arg1	levels					1202:1207	higher mRNA levels	1190:1207	higher mRNA levels for IRX9, IRX10, IRX14L, and FRA8	1190:1241	The knat7 loss-of-function mutants had lower transcript levels of the xylan biosynthetic genes IRX9, IRX10, and FRA8, whereas KNAT7 overexpression plants had higher mRNA levels for IRX9, IRX10, IRX14L, and FRA8.
29393579	1	25	from	polysaccharide	141:154	arg1	wall					178:181	the secondary cell wall	159:181	the secondary cell wall	159:181	Xylan is the major plant hemicellulosic polysaccharide in the secondary cell wall.
29393579	4	26	theme	fiber	703:707	arg1	walls					709:713	thicker interfascicular fiber walls	679:713	thicker interfascicular fiber walls	679:713	The knat7 T-DNA insertion mutants have thinner vessel element walls and xylary fibers, and thicker interfascicular fiber walls in inflorescence stems, relative to wild-type (WT).
29393579	4	27	theme	vessel	635:640	arg1	walls					650:654	thinner vessel element walls	627:654	thinner vessel element walls	627:654	The knat7 T-DNA insertion mutants have thinner vessel element walls and xylary fibers, and thicker interfascicular fiber walls in inflorescence stems, relative to wild-type (WT).
29393579	4	28	contain	have	622:625	arg2	fibers					667:672	xylary fibers	660:672	xylary fibers	660:672	The knat7 T-DNA insertion mutants have thinner vessel element walls and xylary fibers, and thicker interfascicular fiber walls in inflorescence stems, relative to wild-type (WT).
29393579	4	28	contain	have	622:625	arg2	walls					650:654	thinner vessel element walls	627:654	thinner vessel element walls	627:654	The knat7 T-DNA insertion mutants have thinner vessel element walls and xylary fibers, and thicker interfascicular fiber walls in inflorescence stems, relative to wild-type (WT).
29393579	4	28	contain	have	622:625	arg1	mutants					614:620	The knat7 T-DNA insertion mutants	588:620	The knat7 T-DNA insertion mutants	588:620	The knat7 T-DNA insertion mutants have thinner vessel element walls and xylary fibers, and thicker interfascicular fiber walls in inflorescence stems, relative to wild-type (WT).
29393579	4	28	contain	have	622:625	arg2	walls					709:713	thicker interfascicular fiber walls	679:713	thicker interfascicular fiber walls	679:713	The knat7 T-DNA insertion mutants have thinner vessel element walls and xylary fibers, and thicker interfascicular fiber walls in inflorescence stems, relative to wild-type (WT).
29393579	2	29	theme	exact	317:321	arg1	role					323:326	its exact role	313:326	its exact role in regulating xylan biosynthesis	313:359	The transcription factor KNOTTED-LIKE HOMEOBOX OF ARABIDOPSIS THALIANA 7 (KNAT7) regulates secondary cell wall biosynthesis, but its exact role in regulating xylan biosynthesis remains unclear.
29393579	4	30	theme	thinner	627:633	arg1	walls					650:654	thinner vessel element walls	627:654	thinner vessel element walls	627:654	The knat7 T-DNA insertion mutants have thinner vessel element walls and xylary fibers, and thicker interfascicular fiber walls in inflorescence stems, relative to wild-type (WT).
29393579	0	31	theme	xylan	27:31	arg1	biosynthesis					33:44	xylan biosynthesis	27:44	xylan biosynthesis	27:44	KNAT7 positively regulates xylan biosynthesis by directly activating IRX9 expression in Arabidopsis.
29393579	8	32	theme	mobility	1260:1267	arg1	assays					1275:1280	Electrophoretic mobility shift assays	1244:1280	Electrophoretic mobility shift assays	1244:1280	Electrophoretic mobility shift assays indicated that KNAT7 binds to the IRX9 promoter.
29393579	7	33	theme	loss-of-function	1042:1057	arg1	mutants					1059:1065	The knat7 loss-of-function mutants	1032:1065	The knat7 loss-of-function mutants	1032:1065	The knat7 loss-of-function mutants had lower transcript levels of the xylan biosynthetic genes IRX9, IRX10, and FRA8, whereas KNAT7 overexpression plants had higher mRNA levels for IRX9, IRX10, IRX14L, and FRA8.
29393579	2	34	theme	ARABIDOPSIS	234:244	arg1	THALIANA					246:253	ARABIDOPSIS THALIANA 7	234:255	ARABIDOPSIS THALIANA 7 (KNAT7)	234:263	The transcription factor KNOTTED-LIKE HOMEOBOX OF ARABIDOPSIS THALIANA 7 (KNAT7) regulates secondary cell wall biosynthesis, but its exact role in regulating xylan biosynthesis remains unclear.
29393579	2	34	theme	ARABIDOPSIS	234:244	arg1	KNAT7					258:262	KNAT7	258:262	KNAT7	258:262	The transcription factor KNOTTED-LIKE HOMEOBOX OF ARABIDOPSIS THALIANA 7 (KNAT7) regulates secondary cell wall biosynthesis, but its exact role in regulating xylan biosynthesis remains unclear.
29393579	6	35	theme	xylan-specific	1005:1018	arg1	antibodies					1020:1029	xylan-specific antibodies	1005:1029	xylan-specific antibodies	1005:1029	Glycosyl linkage and sugar composition analyses revealed lower xylan levels in knat7 inflorescence stems, relative to WT; a finding supported by labeling of inflorescence walls with xylan-specific antibodies.
29393579	3	36	theme	genes	486:490	arg1	promoters					450:458	the promoters	446:458	the promoters of the xylan biosynthetic genes, IRREGULAR XYLEM 9 (IRX9), IRX10, IRREGULAR XYLEM 14-LIKE (IRX14L), and FRAGILE FIBER 8 (FRA8)	446:585	Using transactivation analyses, we demonstrate that KNAT7 activates the promoters of the xylan biosynthetic genes, IRREGULAR XYLEM 9 (IRX9), IRX10, IRREGULAR XYLEM 14-LIKE (IRX14L), and FRAGILE FIBER 8 (FRA8).
29393579	4	37	theme	interfascicular	687:701	arg1	walls					709:713	thicker interfascicular fiber walls	679:713	thicker interfascicular fiber walls	679:713	The knat7 T-DNA insertion mutants have thinner vessel element walls and xylary fibers, and thicker interfascicular fiber walls in inflorescence stems, relative to wild-type (WT).
29393579	4	38	theme	relative	739:746	arg1	walls					650:654	thinner vessel element walls	627:654	thinner vessel element walls	627:654	The knat7 T-DNA insertion mutants have thinner vessel element walls and xylary fibers, and thicker interfascicular fiber walls in inflorescence stems, relative to wild-type (WT).
29393579	2	39	theme	transcription	188:200	arg1	factor					202:207	The transcription factor	184:207	The transcription factor KNOTTED-LIKE HOMEOBOX OF ARABIDOPSIS THALIANA 7 (KNAT7)	184:263	The transcription factor KNOTTED-LIKE HOMEOBOX OF ARABIDOPSIS THALIANA 7 (KNAT7) regulates secondary cell wall biosynthesis, but its exact role in regulating xylan biosynthesis remains unclear.
29393579	5	40	theme	KNAT7	767:771	arg1	plants					788:793	KNAT7 overexpression plants	767:793	KNAT7 overexpression plants	767:793	KNAT7 overexpression plants exhibited opposite effects.
29393579	7	41	dep	had	1067:1069	arg1	whereas					1150:1156	whereas	1150:1156	whereas	1150:1156	The knat7 loss-of-function mutants had lower transcript levels of the xylan biosynthetic genes IRX9, IRX10, and FRA8, whereas KNAT7 overexpression plants had higher mRNA levels for IRX9, IRX10, IRX14L, and FRA8.
29393579	2	42	theme	xylan	342:346	arg1	biosynthesis					348:359	xylan biosynthesis	342:359	xylan biosynthesis	342:359	The transcription factor KNOTTED-LIKE HOMEOBOX OF ARABIDOPSIS THALIANA 7 (KNAT7) regulates secondary cell wall biosynthesis, but its exact role in regulating xylan biosynthesis remains unclear.
29393579	4	43	theme	insertion	604:612	arg1	mutants					614:620	The knat7 T-DNA insertion mutants	588:620	The knat7 T-DNA insertion mutants	588:620	The knat7 T-DNA insertion mutants have thinner vessel element walls and xylary fibers, and thicker interfascicular fiber walls in inflorescence stems, relative to wild-type (WT).
29393579	4	44	theme	T-DNA	598:602	arg1	mutants					614:620	The knat7 T-DNA insertion mutants	588:620	The knat7 T-DNA insertion mutants	588:620	The knat7 T-DNA insertion mutants have thinner vessel element walls and xylary fibers, and thicker interfascicular fiber walls in inflorescence stems, relative to wild-type (WT).
29393579	3	45	theme	xylan	467:471	arg1	FIBER					572:576	FRAGILE FIBER 8	564:578	FRAGILE FIBER 8 (FRA8)	564:585	Using transactivation analyses, we demonstrate that KNAT7 activates the promoters of the xylan biosynthetic genes, IRREGULAR XYLEM 9 (IRX9), IRX10, IRREGULAR XYLEM 14-LIKE (IRX14L), and FRAGILE FIBER 8 (FRA8).
29393579	3	45	theme	xylan	467:471	arg1	14-LIKE					542:548	IRREGULAR XYLEM 14-LIKE	526:548	IRREGULAR XYLEM 14-LIKE (IRX14L)	526:557	Using transactivation analyses, we demonstrate that KNAT7 activates the promoters of the xylan biosynthetic genes, IRREGULAR XYLEM 9 (IRX9), IRX10, IRREGULAR XYLEM 14-LIKE (IRX14L), and FRAGILE FIBER 8 (FRA8).
29393579	3	45	theme	xylan	467:471	arg1	genes					486:490	the xylan biosynthetic genes	463:490	the xylan biosynthetic genes	463:490	Using transactivation analyses, we demonstrate that KNAT7 activates the promoters of the xylan biosynthetic genes, IRREGULAR XYLEM 9 (IRX9), IRX10, IRREGULAR XYLEM 14-LIKE (IRX14L), and FRAGILE FIBER 8 (FRA8).
29393579	3	45	theme	xylan	467:471	arg1	XYLEM					503:507	IRREGULAR XYLEM 9	493:509	IRREGULAR XYLEM 9 (IRX9)	493:516	Using transactivation analyses, we demonstrate that KNAT7 activates the promoters of the xylan biosynthetic genes, IRREGULAR XYLEM 9 (IRX9), IRX10, IRREGULAR XYLEM 14-LIKE (IRX14L), and FRAGILE FIBER 8 (FRA8).
29393579	3	45	theme	xylan	467:471	arg1	IRX10					519:523	IRX10	519:523	IRX10	519:523	Using transactivation analyses, we demonstrate that KNAT7 activates the promoters of the xylan biosynthetic genes, IRREGULAR XYLEM 9 (IRX9), IRX10, IRREGULAR XYLEM 14-LIKE (IRX14L), and FRAGILE FIBER 8 (FRA8).
29393579	9	46	theme	xylan	1400:1404	arg1	biosynthesis					1406:1417	xylan biosynthesis	1400:1417	xylan biosynthesis	1400:1417	These results support the hypothesis that KNAT7 positively regulates xylan biosynthesis.
29393579	0	47	from	expression	74:83	arg1	Arabidopsis					88:98	Arabidopsis	88:98	Arabidopsis	88:98	KNAT7 positively regulates xylan biosynthesis by directly activating IRX9 expression in Arabidopsis.
29393579	3	48	theme	transactivation	384:398	arg1	analyses					400:407	transactivation analyses	384:407	transactivation analyses	384:407	Using transactivation analyses, we demonstrate that KNAT7 activates the promoters of the xylan biosynthetic genes, IRREGULAR XYLEM 9 (IRX9), IRX10, IRREGULAR XYLEM 14-LIKE (IRX14L), and FRAGILE FIBER 8 (FRA8).
29393579	8	49	theme	Electrophoretic	1244:1258	arg1	assays					1275:1280	Electrophoretic mobility shift assays	1244:1280	Electrophoretic mobility shift assays	1244:1280	Electrophoretic mobility shift assays indicated that KNAT7 binds to the IRX9 promoter.
29393579	3	50	theme	biosynthetic	473:484	arg1	FIBER					572:576	FRAGILE FIBER 8	564:578	FRAGILE FIBER 8 (FRA8)	564:585	Using transactivation analyses, we demonstrate that KNAT7 activates the promoters of the xylan biosynthetic genes, IRREGULAR XYLEM 9 (IRX9), IRX10, IRREGULAR XYLEM 14-LIKE (IRX14L), and FRAGILE FIBER 8 (FRA8).
29393579	3	50	theme	biosynthetic	473:484	arg1	14-LIKE					542:548	IRREGULAR XYLEM 14-LIKE	526:548	IRREGULAR XYLEM 14-LIKE (IRX14L)	526:557	Using transactivation analyses, we demonstrate that KNAT7 activates the promoters of the xylan biosynthetic genes, IRREGULAR XYLEM 9 (IRX9), IRX10, IRREGULAR XYLEM 14-LIKE (IRX14L), and FRAGILE FIBER 8 (FRA8).
29393579	3	50	theme	biosynthetic	473:484	arg1	genes					486:490	the xylan biosynthetic genes	463:490	the xylan biosynthetic genes	463:490	Using transactivation analyses, we demonstrate that KNAT7 activates the promoters of the xylan biosynthetic genes, IRREGULAR XYLEM 9 (IRX9), IRX10, IRREGULAR XYLEM 14-LIKE (IRX14L), and FRAGILE FIBER 8 (FRA8).
29393579	3	50	theme	biosynthetic	473:484	arg1	XYLEM					503:507	IRREGULAR XYLEM 9	493:509	IRREGULAR XYLEM 9 (IRX9)	493:516	Using transactivation analyses, we demonstrate that KNAT7 activates the promoters of the xylan biosynthetic genes, IRREGULAR XYLEM 9 (IRX9), IRX10, IRREGULAR XYLEM 14-LIKE (IRX14L), and FRAGILE FIBER 8 (FRA8).
29393579	3	50	theme	biosynthetic	473:484	arg1	IRX10					519:523	IRX10	519:523	IRX10	519:523	Using transactivation analyses, we demonstrate that KNAT7 activates the promoters of the xylan biosynthetic genes, IRREGULAR XYLEM 9 (IRX9), IRX10, IRREGULAR XYLEM 14-LIKE (IRX14L), and FRAGILE FIBER 8 (FRA8).
29393579	7	51	theme	genes	1121:1125	arg1	levels					1088:1093	lower transcript levels	1071:1093	lower transcript levels of the xylan biosynthetic genes IRX9, IRX10, and FRA8	1071:1147	The knat7 loss-of-function mutants had lower transcript levels of the xylan biosynthetic genes IRX9, IRX10, and FRA8, whereas KNAT7 overexpression plants had higher mRNA levels for IRX9, IRX10, IRX14L, and FRA8.
29393579	7	52	theme	overexpression	1164:1177	arg1	plants					1179:1184	KNAT7 overexpression plants	1158:1184	KNAT7 overexpression plants	1158:1184	The knat7 loss-of-function mutants had lower transcript levels of the xylan biosynthetic genes IRX9, IRX10, and FRA8, whereas KNAT7 overexpression plants had higher mRNA levels for IRX9, IRX10, IRX14L, and FRA8.
29393579	2	53	theme	THALIANA	246:253	arg1	HOMEOBOX					222:229	The transcription factor KNOTTED-LIKE HOMEOBOX	184:229	The transcription factor KNOTTED-LIKE HOMEOBOX OF ARABIDOPSIS THALIANA 7 (KNAT7)	184:263	The transcription factor KNOTTED-LIKE HOMEOBOX OF ARABIDOPSIS THALIANA 7 (KNAT7) regulates secondary cell wall biosynthesis, but its exact role in regulating xylan biosynthesis remains unclear.
29393579	8	54	theme	IRX9	1316:1319	arg1	promoter					1321:1328	the IRX9 promoter	1312:1328	the IRX9 promoter	1312:1328	Electrophoretic mobility shift assays indicated that KNAT7 binds to the IRX9 promoter.
29393579	1	55	theme	major	114:118	arg1	Xylan					101:105	Xylan	101:105	Xylan	101:105	Xylan is the major plant hemicellulosic polysaccharide in the secondary cell wall.
29393579	1	55	theme	major	114:118	arg1	polysaccharide					141:154	the major plant hemicellulosic polysaccharide	110:154	the major plant hemicellulosic polysaccharide in the secondary cell wall	110:181	Xylan is the major plant hemicellulosic polysaccharide in the secondary cell wall.
29393579	6	56	theme	lower	880:884	arg1	levels					892:897	lower xylan levels	880:897	lower xylan levels	880:897	Glycosyl linkage and sugar composition analyses revealed lower xylan levels in knat7 inflorescence stems, relative to WT; a finding supported by labeling of inflorescence walls with xylan-specific antibodies.
29393579	6	57	theme	inflorescence	908:920	arg1	stems					922:926	knat7 inflorescence stems	902:926	knat7 inflorescence stems	902:926	Glycosyl linkage and sugar composition analyses revealed lower xylan levels in knat7 inflorescence stems, relative to WT; a finding supported by labeling of inflorescence walls with xylan-specific antibodies.
29393579	7	58	theme	mRNA	1197:1200	arg1	levels					1202:1207	higher mRNA levels	1190:1207	higher mRNA levels for IRX9, IRX10, IRX14L, and FRA8	1190:1241	The knat7 loss-of-function mutants had lower transcript levels of the xylan biosynthetic genes IRX9, IRX10, and FRA8, whereas KNAT7 overexpression plants had higher mRNA levels for IRX9, IRX10, IRX14L, and FRA8.
29393579	3	59	theme	FRAGILE	564:570	arg1	FIBER					572:576	FRAGILE FIBER 8	564:578	FRAGILE FIBER 8 (FRA8)	564:585	Using transactivation analyses, we demonstrate that KNAT7 activates the promoters of the xylan biosynthetic genes, IRREGULAR XYLEM 9 (IRX9), IRX10, IRREGULAR XYLEM 14-LIKE (IRX14L), and FRAGILE FIBER 8 (FRA8).
29393579	3	59	theme	FRAGILE	564:570	arg1	FRA8					581:584	FRA8	581:584	FRA8	581:584	Using transactivation analyses, we demonstrate that KNAT7 activates the promoters of the xylan biosynthetic genes, IRREGULAR XYLEM 9 (IRX9), IRX10, IRREGULAR XYLEM 14-LIKE (IRX14L), and FRAGILE FIBER 8 (FRA8).
29393579	3	59	theme	FRAGILE	564:570	arg1	genes					486:490	the xylan biosynthetic genes	463:490	the xylan biosynthetic genes	463:490	Using transactivation analyses, we demonstrate that KNAT7 activates the promoters of the xylan biosynthetic genes, IRREGULAR XYLEM 9 (IRX9), IRX10, IRREGULAR XYLEM 14-LIKE (IRX14L), and FRAGILE FIBER 8 (FRA8).
29393579	1	60	theme	plant	120:124	arg1	Xylan					101:105	Xylan	101:105	Xylan	101:105	Xylan is the major plant hemicellulosic polysaccharide in the secondary cell wall.
29393579	1	60	theme	plant	120:124	arg1	polysaccharide					141:154	the major plant hemicellulosic polysaccharide	110:154	the major plant hemicellulosic polysaccharide in the secondary cell wall	110:181	Xylan is the major plant hemicellulosic polysaccharide in the secondary cell wall.
29393579	6	61	theme	knat7	902:906	arg1	stems					922:926	knat7 inflorescence stems	902:926	knat7 inflorescence stems	902:926	Glycosyl linkage and sugar composition analyses revealed lower xylan levels in knat7 inflorescence stems, relative to WT; a finding supported by labeling of inflorescence walls with xylan-specific antibodies.
29393579	2	62	theme	secondary	275:283	arg1	biosynthesis					295:306	secondary cell wall biosynthesis	275:306	secondary cell wall biosynthesis	275:306	The transcription factor KNOTTED-LIKE HOMEOBOX OF ARABIDOPSIS THALIANA 7 (KNAT7) regulates secondary cell wall biosynthesis, but its exact role in regulating xylan biosynthesis remains unclear.
29393579	6	63	theme	inflorescence	980:992	arg1	walls					994:998	inflorescence walls	980:998	inflorescence walls	980:998	Glycosyl linkage and sugar composition analyses revealed lower xylan levels in knat7 inflorescence stems, relative to WT; a finding supported by labeling of inflorescence walls with xylan-specific antibodies.
29393579	1	64	theme	hemicellulosic	126:139	arg1	Xylan					101:105	Xylan	101:105	Xylan	101:105	Xylan is the major plant hemicellulosic polysaccharide in the secondary cell wall.
29393579	1	64	theme	hemicellulosic	126:139	arg1	polysaccharide					141:154	the major plant hemicellulosic polysaccharide	110:154	the major plant hemicellulosic polysaccharide in the secondary cell wall	110:181	Xylan is the major plant hemicellulosic polysaccharide in the secondary cell wall.
29393579	7	65	contain	had	1067:1069	arg2	levels					1088:1093	lower transcript levels	1071:1093	lower transcript levels of the xylan biosynthetic genes IRX9, IRX10, and FRA8	1071:1147	The knat7 loss-of-function mutants had lower transcript levels of the xylan biosynthetic genes IRX9, IRX10, and FRA8, whereas KNAT7 overexpression plants had higher mRNA levels for IRX9, IRX10, IRX14L, and FRA8.
29393579	7	65	contain	had	1067:1069	arg1	mutants					1059:1065	The knat7 loss-of-function mutants	1032:1065	The knat7 loss-of-function mutants	1032:1065	The knat7 loss-of-function mutants had lower transcript levels of the xylan biosynthetic genes IRX9, IRX10, and FRA8, whereas KNAT7 overexpression plants had higher mRNA levels for IRX9, IRX10, IRX14L, and FRA8.
29393579	6	66	theme	walls	994:998	arg1	labeling					968:975	labeling	968:975	labeling of inflorescence walls with xylan-specific antibodies	968:1029	Glycosyl linkage and sugar composition analyses revealed lower xylan levels in knat7 inflorescence stems, relative to WT; a finding supported by labeling of inflorescence walls with xylan-specific antibodies.
29393579	3	67	theme	IRREGULAR	526:534	arg1	IRX14L					551:556	IRX14L	551:556	IRX14L	551:556	Using transactivation analyses, we demonstrate that KNAT7 activates the promoters of the xylan biosynthetic genes, IRREGULAR XYLEM 9 (IRX9), IRX10, IRREGULAR XYLEM 14-LIKE (IRX14L), and FRAGILE FIBER 8 (FRA8).
29393579	3	67	theme	IRREGULAR	526:534	arg1	14-LIKE					542:548	IRREGULAR XYLEM 14-LIKE	526:548	IRREGULAR XYLEM 14-LIKE (IRX14L)	526:557	Using transactivation analyses, we demonstrate that KNAT7 activates the promoters of the xylan biosynthetic genes, IRREGULAR XYLEM 9 (IRX9), IRX10, IRREGULAR XYLEM 14-LIKE (IRX14L), and FRAGILE FIBER 8 (FRA8).
29393579	3	67	theme	IRREGULAR	526:534	arg1	genes					486:490	the xylan biosynthetic genes	463:490	the xylan biosynthetic genes	463:490	Using transactivation analyses, we demonstrate that KNAT7 activates the promoters of the xylan biosynthetic genes, IRREGULAR XYLEM 9 (IRX9), IRX10, IRREGULAR XYLEM 14-LIKE (IRX14L), and FRAGILE FIBER 8 (FRA8).
29393579	2	68	theme	KNOTTED-LIKE	209:220	arg1	HOMEOBOX					222:229	The transcription factor KNOTTED-LIKE HOMEOBOX	184:229	The transcription factor KNOTTED-LIKE HOMEOBOX OF ARABIDOPSIS THALIANA 7 (KNAT7)	184:263	The transcription factor KNOTTED-LIKE HOMEOBOX OF ARABIDOPSIS THALIANA 7 (KNAT7) regulates secondary cell wall biosynthesis, but its exact role in regulating xylan biosynthesis remains unclear.
29393579	4	69	theme	thicker	679:685	arg1	walls					709:713	thicker interfascicular fiber walls	679:713	thicker interfascicular fiber walls	679:713	The knat7 T-DNA insertion mutants have thinner vessel element walls and xylary fibers, and thicker interfascicular fiber walls in inflorescence stems, relative to wild-type (WT).
29393579	8	70	theme	shift	1269:1273	arg1	assays					1275:1280	Electrophoretic mobility shift assays	1244:1280	Electrophoretic mobility shift assays	1244:1280	Electrophoretic mobility shift assays indicated that KNAT7 binds to the IRX9 promoter.
29393579	3	71	theme	IRREGULAR	493:501	arg1	genes					486:490	the xylan biosynthetic genes	463:490	the xylan biosynthetic genes	463:490	Using transactivation analyses, we demonstrate that KNAT7 activates the promoters of the xylan biosynthetic genes, IRREGULAR XYLEM 9 (IRX9), IRX10, IRREGULAR XYLEM 14-LIKE (IRX14L), and FRAGILE FIBER 8 (FRA8).
29393579	3	71	theme	IRREGULAR	493:501	arg1	XYLEM					503:507	IRREGULAR XYLEM 9	493:509	IRREGULAR XYLEM 9 (IRX9)	493:516	Using transactivation analyses, we demonstrate that KNAT7 activates the promoters of the xylan biosynthetic genes, IRREGULAR XYLEM 9 (IRX9), IRX10, IRREGULAR XYLEM 14-LIKE (IRX14L), and FRAGILE FIBER 8 (FRA8).
29393579	3	71	theme	IRREGULAR	493:501	arg1	IRX9					512:515	IRX9	512:515	IRX9	512:515	Using transactivation analyses, we demonstrate that KNAT7 activates the promoters of the xylan biosynthetic genes, IRREGULAR XYLEM 9 (IRX9), IRX10, IRREGULAR XYLEM 14-LIKE (IRX14L), and FRAGILE FIBER 8 (FRA8).
29393579	6	72	theme	linkage	832:838	arg1	analyses					862:869	Glycosyl linkage and sugar composition analyses	823:869	Glycosyl linkage and sugar composition analyses	823:869	Glycosyl linkage and sugar composition analyses revealed lower xylan levels in knat7 inflorescence stems, relative to WT; a finding supported by labeling of inflorescence walls with xylan-specific antibodies.
29393579	2	73	theme	factor	202:207	arg1	HOMEOBOX					222:229	The transcription factor KNOTTED-LIKE HOMEOBOX	184:229	The transcription factor KNOTTED-LIKE HOMEOBOX OF ARABIDOPSIS THALIANA 7 (KNAT7)	184:263	The transcription factor KNOTTED-LIKE HOMEOBOX OF ARABIDOPSIS THALIANA 7 (KNAT7) regulates secondary cell wall biosynthesis, but its exact role in regulating xylan biosynthesis remains unclear.
29393579	4	74	theme	knat7	592:596	arg1	mutants					614:620	The knat7 T-DNA insertion mutants	588:620	The knat7 T-DNA insertion mutants	588:620	The knat7 T-DNA insertion mutants have thinner vessel element walls and xylary fibers, and thicker interfascicular fiber walls in inflorescence stems, relative to wild-type (WT).
29393579	4	75	from	fibers	667:672	arg1	stems					732:736	inflorescence stems	718:736	inflorescence stems	718:736	The knat7 T-DNA insertion mutants have thinner vessel element walls and xylary fibers, and thicker interfascicular fiber walls in inflorescence stems, relative to wild-type (WT).
29393579	6	76	dep	revealed	871:878	arg1	finding					947:953	a finding	945:953	a finding supported by labeling of inflorescence walls with xylan-specific antibodies	945:1029	Glycosyl linkage and sugar composition analyses revealed lower xylan levels in knat7 inflorescence stems, relative to WT; a finding supported by labeling of inflorescence walls with xylan-specific antibodies.
29393579	0	77	theme	IRX9	69:72	arg1	expression					74:83	IRX9 expression	69:83	IRX9 expression in Arabidopsis	69:98	KNAT7 positively regulates xylan biosynthesis by directly activating IRX9 expression in Arabidopsis.
29393579	5	78	theme	overexpression	773:786	arg1	plants					788:793	KNAT7 overexpression plants	767:793	KNAT7 overexpression plants	767:793	KNAT7 overexpression plants exhibited opposite effects.
29393579	4	79	theme	element	642:648	arg1	walls					650:654	thinner vessel element walls	627:654	thinner vessel element walls	627:654	The knat7 T-DNA insertion mutants have thinner vessel element walls and xylary fibers, and thicker interfascicular fiber walls in inflorescence stems, relative to wild-type (WT).
29393579	7	80	dep	genes	1121:1125	arg1	genes					1121:1125	xylan biosynthetic genes	1102:1125	the xylan biosynthetic genes IRX9, IRX10, and FRA8	1098:1147	The knat7 loss-of-function mutants had lower transcript levels of the xylan biosynthetic genes IRX9, IRX10, and FRA8, whereas KNAT7 overexpression plants had higher mRNA levels for IRX9, IRX10, IRX14L, and FRA8.
29393579	7	80	dep	genes	1121:1125	arg1	IRX10					1133:1137	IRX10	1133:1137	IRX10	1133:1137	The knat7 loss-of-function mutants had lower transcript levels of the xylan biosynthetic genes IRX9, IRX10, and FRA8, whereas KNAT7 overexpression plants had higher mRNA levels for IRX9, IRX10, IRX14L, and FRA8.
29393579	7	80	dep	genes	1121:1125	arg1	FRA8					1144:1147	FRA8	1144:1147	FRA8	1144:1147	The knat7 loss-of-function mutants had lower transcript levels of the xylan biosynthetic genes IRX9, IRX10, and FRA8, whereas KNAT7 overexpression plants had higher mRNA levels for IRX9, IRX10, IRX14L, and FRA8.
29393579	7	80	dep	genes	1121:1125	arg1	IRX9					1127:1130	IRX9	1127:1130	IRX9	1127:1130	The knat7 loss-of-function mutants had lower transcript levels of the xylan biosynthetic genes IRX9, IRX10, and FRA8, whereas KNAT7 overexpression plants had higher mRNA levels for IRX9, IRX10, IRX14L, and FRA8.
31074673	4	0	theme	sulfate	788:794	arg1	sodium					796:801	dextran sulfate sodium	780:801	dextran sulfate sodium (DSS)	780:807	This study evaluated the phenolic profile of CA and the effects of the daily consumption of CA leaf infusion on the formation of ACF, histopathological lesions, and molecular biomarkers after azoxymethane (AOM) and dextran sulfate sodium (DSS) induced premalignant colon lesions in rats treated with for 16 and 32 weeks.
31074673	4	0	theme	sulfate	788:794	arg1	DSS					804:806	DSS	804:806	DSS	804:806	This study evaluated the phenolic profile of CA and the effects of the daily consumption of CA leaf infusion on the formation of ACF, histopathological lesions, and molecular biomarkers after azoxymethane (AOM) and dextran sulfate sodium (DSS) induced premalignant colon lesions in rats treated with for 16 and 32 weeks.
31074673	10	1	theme	preventive	1609:1618	arg1	effects					1620:1626	preventive effects	1609:1626	preventive effects against AOM-/DSS-induced premalignant colon lesions in colon rats	1609:1692	Our data suggest that CA treatment has preventive effects against AOM-/DSS-induced premalignant colon lesions in colon rats at the promotion level, inhibiting the cell proliferation of early neoplastic lesions and colonic inflammation through the decrease of β-catenin by 41.8% at the subchronic stage and 29% at the chronic stage, along with a 46.2% reduction of cyclooxygenase 2 (COX-2) at long term, despite a high expression of NF-κB (30.3% at the subchronic stage and 22.8% at the chronic stage).
31074673	10	2	theme	chronic	1887:1893	arg1	stage					1895:1899	the chronic stage	1883:1899	the chronic stage	1883:1899	Our data suggest that CA treatment has preventive effects against AOM-/DSS-induced premalignant colon lesions in colon rats at the promotion level, inhibiting the cell proliferation of early neoplastic lesions and colonic inflammation through the decrease of β-catenin by 41.8% at the subchronic stage and 29% at the chronic stage, along with a 46.2% reduction of cyclooxygenase 2 (COX-2) at long term, despite a high expression of NF-κB (30.3% at the subchronic stage and 22.8% at the chronic stage).
31074673	10	3	from	stage	1895:1899	arg1	%					1846:1846	41.8%	1842:1846	41.8% at the subchronic stage	1842:1870	Our data suggest that CA treatment has preventive effects against AOM-/DSS-induced premalignant colon lesions in colon rats at the promotion level, inhibiting the cell proliferation of early neoplastic lesions and colonic inflammation through the decrease of β-catenin by 41.8% at the subchronic stage and 29% at the chronic stage, along with a 46.2% reduction of cyclooxygenase 2 (COX-2) at long term, despite a high expression of NF-κB (30.3% at the subchronic stage and 22.8% at the chronic stage).
31074673	10	3	from	stage	1895:1899	arg1	%					1878:1878	29%	1876:1878	29% at the chronic stage	1876:1899	Our data suggest that CA treatment has preventive effects against AOM-/DSS-induced premalignant colon lesions in colon rats at the promotion level, inhibiting the cell proliferation of early neoplastic lesions and colonic inflammation through the decrease of β-catenin by 41.8% at the subchronic stage and 29% at the chronic stage, along with a 46.2% reduction of cyclooxygenase 2 (COX-2) at long term, despite a high expression of NF-κB (30.3% at the subchronic stage and 22.8% at the chronic stage).
31074673	10	4	theme	lesions	1772:1778	arg1	inflammation					1792:1803	colonic inflammation	1784:1803	colonic inflammation	1784:1803	Our data suggest that CA treatment has preventive effects against AOM-/DSS-induced premalignant colon lesions in colon rats at the promotion level, inhibiting the cell proliferation of early neoplastic lesions and colonic inflammation through the decrease of β-catenin by 41.8% at the subchronic stage and 29% at the chronic stage, along with a 46.2% reduction of cyclooxygenase 2 (COX-2) at long term, despite a high expression of NF-κB (30.3% at the subchronic stage and 22.8% at the chronic stage).
31074673	10	4	theme	lesions	1772:1778	arg1	proliferation					1738:1750	the cell proliferation	1729:1750	the cell proliferation of early neoplastic lesions	1729:1778	Our data suggest that CA treatment has preventive effects against AOM-/DSS-induced premalignant colon lesions in colon rats at the promotion level, inhibiting the cell proliferation of early neoplastic lesions and colonic inflammation through the decrease of β-catenin by 41.8% at the subchronic stage and 29% at the chronic stage, along with a 46.2% reduction of cyclooxygenase 2 (COX-2) at long term, despite a high expression of NF-κB (30.3% at the subchronic stage and 22.8% at the chronic stage).
31074673	6	5	theme	blue	1146:1149	arg1	methylene					1136:1144	methylene blue	1136:1149	methylene blue to look for ACF	1136:1165	After sacrifice, a 4 cm segment was collected from the distal part of the colon and stained with methylene blue to look for ACF.
31074673	1	6	theme	precursor	221:229	arg1	lesion					231:236	the precursor lesion	217:236	the precursor lesion of colorectal carcinogenesis (CRC), one of the most common malignancies in the world	217:321	Aberrant crypt foci (ACF) is the precursor lesion of colorectal carcinogenesis (CRC), one of the most common malignancies in the world.
31074673	1	6	theme	precursor	221:229	arg1	foci					203:206	Aberrant crypt foci	188:206	Aberrant crypt foci (ACF)	188:212	Aberrant crypt foci (ACF) is the precursor lesion of colorectal carcinogenesis (CRC), one of the most common malignancies in the world.
31074673	4	7	theme	biomarkers	740:749	arg1	formation					681:689	the formation	677:689	the formation of ACF, histopathological lesions, and molecular biomarkers after azoxymethane (AOM) and dextran sulfate sodium (DSS)	677:807	This study evaluated the phenolic profile of CA and the effects of the daily consumption of CA leaf infusion on the formation of ACF, histopathological lesions, and molecular biomarkers after azoxymethane (AOM) and dextran sulfate sodium (DSS) induced premalignant colon lesions in rats treated with for 16 and 32 weeks.
31074673	6	8	theme	4 cm	1058:1061	arg1	segment					1063:1069	a 4 cm segment	1056:1069	a 4 cm segment	1056:1069	After sacrifice, a 4 cm segment was collected from the distal part of the colon and stained with methylene blue to look for ACF.
31074673	10	9	theme	early	1755:1759	arg1	lesions					1772:1778	early neoplastic lesions	1755:1778	early neoplastic lesions	1755:1778	Our data suggest that CA treatment has preventive effects against AOM-/DSS-induced premalignant colon lesions in colon rats at the promotion level, inhibiting the cell proliferation of early neoplastic lesions and colonic inflammation through the decrease of β-catenin by 41.8% at the subchronic stage and 29% at the chronic stage, along with a 46.2% reduction of cyclooxygenase 2 (COX-2) at long term, despite a high expression of NF-κB (30.3% at the subchronic stage and 22.8% at the chronic stage).
31074673	3	10	theme	botanical	456:464	arg1	potential					466:474	the botanical potential	452:474	the botanical potential of preventive plant products	452:503	One example of the botanical potential of preventive plant products is Cnidoscolus aconitifolius (CA), commonly known as Chaya.
31074673	4	11	theme	phenolic	590:597	arg1	profile					599:605	the phenolic profile	586:605	the phenolic profile of CA	586:611	This study evaluated the phenolic profile of CA and the effects of the daily consumption of CA leaf infusion on the formation of ACF, histopathological lesions, and molecular biomarkers after azoxymethane (AOM) and dextran sulfate sodium (DSS) induced premalignant colon lesions in rats treated with for 16 and 32 weeks.
31074673	10	12	theme	CA	1592:1593	arg1	treatment					1595:1603	CA treatment	1592:1603	CA treatment	1592:1603	Our data suggest that CA treatment has preventive effects against AOM-/DSS-induced premalignant colon lesions in colon rats at the promotion level, inhibiting the cell proliferation of early neoplastic lesions and colonic inflammation through the decrease of β-catenin by 41.8% at the subchronic stage and 29% at the chronic stage, along with a 46.2% reduction of cyclooxygenase 2 (COX-2) at long term, despite a high expression of NF-κB (30.3% at the subchronic stage and 22.8% at the chronic stage).
31074673	4	13	theme	infusion	665:672	arg1	consumption					642:652	the daily consumption	632:652	the daily consumption of CA leaf infusion	632:672	This study evaluated the phenolic profile of CA and the effects of the daily consumption of CA leaf infusion on the formation of ACF, histopathological lesions, and molecular biomarkers after azoxymethane (AOM) and dextran sulfate sodium (DSS) induced premalignant colon lesions in rats treated with for 16 and 32 weeks.
31074673	10	14	theme	cyclooxygenase	1934:1947	arg1	reduction					1921:1929	a 46.2% reduction	1913:1929	a 46.2% reduction of cyclooxygenase 2 (COX-2) at long term	1913:1970	Our data suggest that CA treatment has preventive effects against AOM-/DSS-induced premalignant colon lesions in colon rats at the promotion level, inhibiting the cell proliferation of early neoplastic lesions and colonic inflammation through the decrease of β-catenin by 41.8% at the subchronic stage and 29% at the chronic stage, along with a 46.2% reduction of cyclooxygenase 2 (COX-2) at long term, despite a high expression of NF-κB (30.3% at the subchronic stage and 22.8% at the chronic stage).
31074673	10	15	from	term	1967:1970	arg1	reduction					1921:1929	a 46.2% reduction	1913:1929	a 46.2% reduction of cyclooxygenase 2 (COX-2) at long term	1913:1970	Our data suggest that CA treatment has preventive effects against AOM-/DSS-induced premalignant colon lesions in colon rats at the promotion level, inhibiting the cell proliferation of early neoplastic lesions and colonic inflammation through the decrease of β-catenin by 41.8% at the subchronic stage and 29% at the chronic stage, along with a 46.2% reduction of cyclooxygenase 2 (COX-2) at long term, despite a high expression of NF-κB (30.3% at the subchronic stage and 22.8% at the chronic stage).
31074673	10	16	theme	colonic	1784:1790	arg1	inflammation					1792:1803	colonic inflammation	1784:1803	colonic inflammation	1784:1803	Our data suggest that CA treatment has preventive effects against AOM-/DSS-induced premalignant colon lesions in colon rats at the promotion level, inhibiting the cell proliferation of early neoplastic lesions and colonic inflammation through the decrease of β-catenin by 41.8% at the subchronic stage and 29% at the chronic stage, along with a 46.2% reduction of cyclooxygenase 2 (COX-2) at long term, despite a high expression of NF-κB (30.3% at the subchronic stage and 22.8% at the chronic stage).
31074673	3	17	theme	plant	490:494	arg1	products					496:503	preventive plant products	479:503	preventive plant products	479:503	One example of the botanical potential of preventive plant products is Cnidoscolus aconitifolius (CA), commonly known as Chaya.
31074673	9	18	from	Lesions	1508:1514	arg1	tissue					1544:1549	colon tissue	1538:1549	colon tissue	1538:1549	Lesions on kidney, liver, and colon tissue were also reduced.
31074673	9	18	from	Lesions	1508:1514	arg1	liver					1527:1531	liver	1527:1531	liver	1527:1531	Lesions on kidney, liver, and colon tissue were also reduced.
31074673	9	18	from	Lesions	1508:1514	arg1	kidney					1519:1524	kidney	1519:1524	kidney	1519:1524	Lesions on kidney, liver, and colon tissue were also reduced.
31074673	4	19	theme	premalignant	817:828	arg1	lesions					836:842	premalignant colon lesions	817:842	premalignant colon lesions	817:842	This study evaluated the phenolic profile of CA and the effects of the daily consumption of CA leaf infusion on the formation of ACF, histopathological lesions, and molecular biomarkers after azoxymethane (AOM) and dextran sulfate sodium (DSS) induced premalignant colon lesions in rats treated with for 16 and 32 weeks.
31074673	6	20	theme	colon	1113:1117	arg1	part					1101:1104	the distal part	1090:1104	the distal part of the colon	1090:1117	After sacrifice, a 4 cm segment was collected from the distal part of the colon and stained with methylene blue to look for ACF.
31074673	10	21	theme	β-catenin	1829:1837	arg1	decrease					1817:1824	the decrease	1813:1824	the decrease of β-catenin by 41.8% at the subchronic stage and 29% at the chronic stage	1813:1899	Our data suggest that CA treatment has preventive effects against AOM-/DSS-induced premalignant colon lesions in colon rats at the promotion level, inhibiting the cell proliferation of early neoplastic lesions and colonic inflammation through the decrease of β-catenin by 41.8% at the subchronic stage and 29% at the chronic stage, along with a 46.2% reduction of cyclooxygenase 2 (COX-2) at long term, despite a high expression of NF-κB (30.3% at the subchronic stage and 22.8% at the chronic stage).
31074673	0	22	theme	Aberrant	106:113	arg1	Model					130:134	an Aberrant Crypt Foci Rat Model	103:134	an Aberrant Crypt Foci Rat Model Induced by Azoxymethane and Dextran Sulfate Sodium	103:185	Preventive Effect of an Infusion of the Aqueous Extract of Chaya Leaves (Cnidoscolus aconitifolius) in an Aberrant Crypt Foci Rat Model Induced by Azoxymethane and Dextran Sulfate Sodium.
31074673	5	23	theme	reversed	948:955	arg1	RP-HPCC-DAD					1025:1035	RP-HPCC-DAD	1025:1035	RP-HPCC-DAD	1025:1035	The phenolic composition of the CA infusion was identified by reversed phase-high performance liquid chromatography-diode array detection (RP-HPCC-DAD).
31074673	5	23	theme	reversed	948:955	arg1	detection					1014:1022	reversed phase-high performance liquid chromatography-diode array detection	948:1022	reversed phase-high performance liquid chromatography-diode array detection (RP-HPCC-DAD)	948:1036	The phenolic composition of the CA infusion was identified by reversed phase-high performance liquid chromatography-diode array detection (RP-HPCC-DAD).
31074673	1	24	theme	Aberrant	188:195	arg1	lesion					231:236	the precursor lesion	217:236	the precursor lesion of colorectal carcinogenesis (CRC), one of the most common malignancies in the world	217:321	Aberrant crypt foci (ACF) is the precursor lesion of colorectal carcinogenesis (CRC), one of the most common malignancies in the world.
31074673	1	24	theme	Aberrant	188:195	arg1	ACF					209:211	ACF	209:211	ACF	209:211	Aberrant crypt foci (ACF) is the precursor lesion of colorectal carcinogenesis (CRC), one of the most common malignancies in the world.
31074673	1	24	theme	Aberrant	188:195	arg1	foci					203:206	Aberrant crypt foci	188:206	Aberrant crypt foci (ACF)	188:212	Aberrant crypt foci (ACF) is the precursor lesion of colorectal carcinogenesis (CRC), one of the most common malignancies in the world.
31074673	9	25	theme	colon	1538:1542	arg1	tissue					1544:1549	colon tissue	1538:1549	colon tissue	1538:1549	Lesions on kidney, liver, and colon tissue were also reduced.
31074673	0	26	theme	Foci	121:124	arg1	Model					130:134	an Aberrant Crypt Foci Rat Model	103:134	an Aberrant Crypt Foci Rat Model Induced by Azoxymethane and Dextran Sulfate Sodium	103:185	Preventive Effect of an Infusion of the Aqueous Extract of Chaya Leaves (Cnidoscolus aconitifolius) in an Aberrant Crypt Foci Rat Model Induced by Azoxymethane and Dextran Sulfate Sodium.
31074673	6	27	theme	distal	1094:1099	arg1	part					1101:1104	the distal part	1090:1104	the distal part of the colon	1090:1117	After sacrifice, a 4 cm segment was collected from the distal part of the colon and stained with methylene blue to look for ACF.
31074673	5	28	theme	infusion	921:928	arg1	composition					899:909	The phenolic composition	886:909	The phenolic composition of the CA infusion	886:928	The phenolic composition of the CA infusion was identified by reversed phase-high performance liquid chromatography-diode array detection (RP-HPCC-DAD).
31074673	10	29	theme	colon	1666:1670	arg1	lesions					1672:1678	AOM-/DSS-induced premalignant colon lesions	1636:1678	AOM-/DSS-induced premalignant colon lesions	1636:1678	Our data suggest that CA treatment has preventive effects against AOM-/DSS-induced premalignant colon lesions in colon rats at the promotion level, inhibiting the cell proliferation of early neoplastic lesions and colonic inflammation through the decrease of β-catenin by 41.8% at the subchronic stage and 29% at the chronic stage, along with a 46.2% reduction of cyclooxygenase 2 (COX-2) at long term, despite a high expression of NF-κB (30.3% at the subchronic stage and 22.8% at the chronic stage).
31074673	2	30	theme	ACF	432:434	arg1	risk					413:416	a reduced risk	403:416	a reduced risk of developing ACF	403:434	Many studies have reported that people with higher phytochemical intake are at a reduced risk of developing ACF.
31074673	7	31	theme	liver	1196:1200	arg1	4 μm					1181:1184	4 μm	1181:1184	4 μm of colon, liver, and kidney	1181:1212	Furthermore, 4 μm of colon, liver, and kidney was collected and stained with hematoxylin and eosin for histopathological analysis, along with 7 μm of colon for immunohistochemistry analysis.
31074673	0	32	theme	Extract	48:54	arg1	Infusion					24:31	an Infusion	21:31	an Infusion of the Aqueous Extract of Chaya	21:63	Preventive Effect of an Infusion of the Aqueous Extract of Chaya Leaves (Cnidoscolus aconitifolius) in an Aberrant Crypt Foci Rat Model Induced by Azoxymethane and Dextran Sulfate Sodium.
31074673	10	33	theme	AOM-/DSS-induced	1636:1651	arg1	lesions					1672:1678	AOM-/DSS-induced premalignant colon lesions	1636:1678	AOM-/DSS-induced premalignant colon lesions	1636:1678	Our data suggest that CA treatment has preventive effects against AOM-/DSS-induced premalignant colon lesions in colon rats at the promotion level, inhibiting the cell proliferation of early neoplastic lesions and colonic inflammation through the decrease of β-catenin by 41.8% at the subchronic stage and 29% at the chronic stage, along with a 46.2% reduction of cyclooxygenase 2 (COX-2) at long term, despite a high expression of NF-κB (30.3% at the subchronic stage and 22.8% at the chronic stage).
31074673	10	34	from	stage	2033:2037	arg1	%					2047:2047	22.8%	2043:2047	22.8% at the chronic stage	2043:2068	Our data suggest that CA treatment has preventive effects against AOM-/DSS-induced premalignant colon lesions in colon rats at the promotion level, inhibiting the cell proliferation of early neoplastic lesions and colonic inflammation through the decrease of β-catenin by 41.8% at the subchronic stage and 29% at the chronic stage, along with a 46.2% reduction of cyclooxygenase 2 (COX-2) at long term, despite a high expression of NF-κB (30.3% at the subchronic stage and 22.8% at the chronic stage).
31074673	10	34	from	stage	2033:2037	arg1	%					2013:2013	30.3%	2009:2013	30.3% at the subchronic stage	2009:2037	Our data suggest that CA treatment has preventive effects against AOM-/DSS-induced premalignant colon lesions in colon rats at the promotion level, inhibiting the cell proliferation of early neoplastic lesions and colonic inflammation through the decrease of β-catenin by 41.8% at the subchronic stage and 29% at the chronic stage, along with a 46.2% reduction of cyclooxygenase 2 (COX-2) at long term, despite a high expression of NF-κB (30.3% at the subchronic stage and 22.8% at the chronic stage).
31074673	10	35	theme	long	1962:1965	arg1	term					1967:1970	long term	1962:1970	long term	1962:1970	Our data suggest that CA treatment has preventive effects against AOM-/DSS-induced premalignant colon lesions in colon rats at the promotion level, inhibiting the cell proliferation of early neoplastic lesions and colonic inflammation through the decrease of β-catenin by 41.8% at the subchronic stage and 29% at the chronic stage, along with a 46.2% reduction of cyclooxygenase 2 (COX-2) at long term, despite a high expression of NF-κB (30.3% at the subchronic stage and 22.8% at the chronic stage).
31074673	0	36	theme	Chaya	59:63	arg1	Extract					48:54	the Aqueous Extract	36:54	the Aqueous Extract of Chaya	36:63	Preventive Effect of an Infusion of the Aqueous Extract of Chaya Leaves (Cnidoscolus aconitifolius) in an Aberrant Crypt Foci Rat Model Induced by Azoxymethane and Dextran Sulfate Sodium.
31074673	8	37	theme	phenolic	1366:1373	arg1	compounds					1375:1383	Eleven phenolic compounds	1359:1383	Eleven phenolic compounds	1359:1383	Eleven phenolic compounds were identified in the infusions, and ACF formation was reduced by 29.5% at the subchronic and by 64.6% at chronic stages.
31074673	8	38	from	stages	1500:1505	arg1	subchronic					1465:1474	subchronic	1465:1474	subchronic	1465:1474	Eleven phenolic compounds were identified in the infusions, and ACF formation was reduced by 29.5% at the subchronic and by 64.6% at chronic stages.
31074673	10	39	theme	subchronic	1855:1864	arg1	stage					1866:1870	the subchronic stage	1851:1870	the subchronic stage	1851:1870	Our data suggest that CA treatment has preventive effects against AOM-/DSS-induced premalignant colon lesions in colon rats at the promotion level, inhibiting the cell proliferation of early neoplastic lesions and colonic inflammation through the decrease of β-catenin by 41.8% at the subchronic stage and 29% at the chronic stage, along with a 46.2% reduction of cyclooxygenase 2 (COX-2) at long term, despite a high expression of NF-κB (30.3% at the subchronic stage and 22.8% at the chronic stage).
31074673	5	40	theme	performance	968:978	arg1	RP-HPCC-DAD					1025:1035	RP-HPCC-DAD	1025:1035	RP-HPCC-DAD	1025:1035	The phenolic composition of the CA infusion was identified by reversed phase-high performance liquid chromatography-diode array detection (RP-HPCC-DAD).
31074673	5	40	theme	performance	968:978	arg1	detection					1014:1022	reversed phase-high performance liquid chromatography-diode array detection	948:1022	reversed phase-high performance liquid chromatography-diode array detection (RP-HPCC-DAD)	948:1036	The phenolic composition of the CA infusion was identified by reversed phase-high performance liquid chromatography-diode array detection (RP-HPCC-DAD).
31074673	5	41	theme	chromatography-diode	987:1006	arg1	RP-HPCC-DAD					1025:1035	RP-HPCC-DAD	1025:1035	RP-HPCC-DAD	1025:1035	The phenolic composition of the CA infusion was identified by reversed phase-high performance liquid chromatography-diode array detection (RP-HPCC-DAD).
31074673	5	41	theme	chromatography-diode	987:1006	arg1	detection					1014:1022	reversed phase-high performance liquid chromatography-diode array detection	948:1022	reversed phase-high performance liquid chromatography-diode array detection (RP-HPCC-DAD)	948:1036	The phenolic composition of the CA infusion was identified by reversed phase-high performance liquid chromatography-diode array detection (RP-HPCC-DAD).
31074673	10	42	from	effects	1620:1626	arg1	rats					1689:1692	colon rats	1683:1692	colon rats	1683:1692	Our data suggest that CA treatment has preventive effects against AOM-/DSS-induced premalignant colon lesions in colon rats at the promotion level, inhibiting the cell proliferation of early neoplastic lesions and colonic inflammation through the decrease of β-catenin by 41.8% at the subchronic stage and 29% at the chronic stage, along with a 46.2% reduction of cyclooxygenase 2 (COX-2) at long term, despite a high expression of NF-κB (30.3% at the subchronic stage and 22.8% at the chronic stage).
31074673	10	43	contain	has	1605:1607	arg2	effects					1620:1626	preventive effects	1609:1626	preventive effects against AOM-/DSS-induced premalignant colon lesions in colon rats	1609:1692	Our data suggest that CA treatment has preventive effects against AOM-/DSS-induced premalignant colon lesions in colon rats at the promotion level, inhibiting the cell proliferation of early neoplastic lesions and colonic inflammation through the decrease of β-catenin by 41.8% at the subchronic stage and 29% at the chronic stage, along with a 46.2% reduction of cyclooxygenase 2 (COX-2) at long term, despite a high expression of NF-κB (30.3% at the subchronic stage and 22.8% at the chronic stage).
31074673	10	43	contain	has	1605:1607	arg1	treatment					1595:1603	CA treatment	1592:1603	CA treatment	1592:1603	Our data suggest that CA treatment has preventive effects against AOM-/DSS-induced premalignant colon lesions in colon rats at the promotion level, inhibiting the cell proliferation of early neoplastic lesions and colonic inflammation through the decrease of β-catenin by 41.8% at the subchronic stage and 29% at the chronic stage, along with a 46.2% reduction of cyclooxygenase 2 (COX-2) at long term, despite a high expression of NF-κB (30.3% at the subchronic stage and 22.8% at the chronic stage).
31074673	4	44	theme	consumption	642:652	arg1	effects					621:627	the effects	617:627	the effects of the daily consumption of CA leaf infusion on the formation of ACF, histopathological lesions, and molecular biomarkers after azoxymethane (AOM) and dextran sulfate sodium (DSS)	617:807	This study evaluated the phenolic profile of CA and the effects of the daily consumption of CA leaf infusion on the formation of ACF, histopathological lesions, and molecular biomarkers after azoxymethane (AOM) and dextran sulfate sodium (DSS) induced premalignant colon lesions in rats treated with for 16 and 32 weeks.
31074673	0	45	theme	Preventive	0:9	arg1	Effect					11:16	Preventive Effect	0:16	Preventive Effect of an Infusion of the Aqueous Extract of Chaya	0:63	Preventive Effect of an Infusion of the Aqueous Extract of Chaya Leaves (Cnidoscolus aconitifolius) in an Aberrant Crypt Foci Rat Model Induced by Azoxymethane and Dextran Sulfate Sodium.
31074673	2	46	theme	higher	368:373	arg1	intake					389:394	higher phytochemical intake	368:394	higher phytochemical intake	368:394	Many studies have reported that people with higher phytochemical intake are at a reduced risk of developing ACF.
31074673	10	47	theme	NF-κB	2002:2006	arg1	expression					1988:1997	a high expression	1981:1997	a high expression of NF-κB (30.3% at the subchronic stage and 22.8% at the chronic stage)	1981:2069	Our data suggest that CA treatment has preventive effects against AOM-/DSS-induced premalignant colon lesions in colon rats at the promotion level, inhibiting the cell proliferation of early neoplastic lesions and colonic inflammation through the decrease of β-catenin by 41.8% at the subchronic stage and 29% at the chronic stage, along with a 46.2% reduction of cyclooxygenase 2 (COX-2) at long term, despite a high expression of NF-κB (30.3% at the subchronic stage and 22.8% at the chronic stage).
31074673	4	48	theme	CA	657:658	arg1	infusion					665:672	CA leaf infusion	657:672	CA leaf infusion	657:672	This study evaluated the phenolic profile of CA and the effects of the daily consumption of CA leaf infusion on the formation of ACF, histopathological lesions, and molecular biomarkers after azoxymethane (AOM) and dextran sulfate sodium (DSS) induced premalignant colon lesions in rats treated with for 16 and 32 weeks.
31074673	1	49	from	malignancies	297:308	arg1	world					317:321	the world	313:321	the world	313:321	Aberrant crypt foci (ACF) is the precursor lesion of colorectal carcinogenesis (CRC), one of the most common malignancies in the world.
31074673	1	50	theme	colorectal	241:250	arg1	CRC					268:270	CRC	268:270	CRC	268:270	Aberrant crypt foci (ACF) is the precursor lesion of colorectal carcinogenesis (CRC), one of the most common malignancies in the world.
31074673	1	50	theme	colorectal	241:250	arg1	one					274:276	one	274:276	one	274:276	Aberrant crypt foci (ACF) is the precursor lesion of colorectal carcinogenesis (CRC), one of the most common malignancies in the world.
31074673	1	50	theme	colorectal	241:250	arg1	carcinogenesis					252:265	colorectal carcinogenesis	241:265	colorectal carcinogenesis (CRC)	241:271	Aberrant crypt foci (ACF) is the precursor lesion of colorectal carcinogenesis (CRC), one of the most common malignancies in the world.
31074673	1	50	theme	colorectal	241:250	arg1	malignancies					297:308	the most common malignancies	281:308	the most common malignancies in the world	281:321	Aberrant crypt foci (ACF) is the precursor lesion of colorectal carcinogenesis (CRC), one of the most common malignancies in the world.
31074673	2	51	theme	reduced	405:411	arg1	risk					413:416	a reduced risk	403:416	a reduced risk of developing ACF	403:434	Many studies have reported that people with higher phytochemical intake are at a reduced risk of developing ACF.
31074673	4	52	theme	lesions	717:723	arg1	formation					681:689	the formation	677:689	the formation of ACF, histopathological lesions, and molecular biomarkers after azoxymethane (AOM) and dextran sulfate sodium (DSS)	677:807	This study evaluated the phenolic profile of CA and the effects of the daily consumption of CA leaf infusion on the formation of ACF, histopathological lesions, and molecular biomarkers after azoxymethane (AOM) and dextran sulfate sodium (DSS) induced premalignant colon lesions in rats treated with for 16 and 32 weeks.
31074673	0	53	theme	Infusion	24:31	arg1	Effect					11:16	Preventive Effect	0:16	Preventive Effect of an Infusion of the Aqueous Extract of Chaya	0:63	Preventive Effect of an Infusion of the Aqueous Extract of Chaya Leaves (Cnidoscolus aconitifolius) in an Aberrant Crypt Foci Rat Model Induced by Azoxymethane and Dextran Sulfate Sodium.
31074673	7	54	dep	along	1299:1303	arg1	with					1305:1308	with	1305:1308	with	1305:1308	Furthermore, 4 μm of colon, liver, and kidney was collected and stained with hematoxylin and eosin for histopathological analysis, along with 7 μm of colon for immunohistochemistry analysis.
31074673	5	55	theme	phenolic	890:897	arg1	composition					899:909	The phenolic composition	886:909	The phenolic composition of the CA infusion	886:928	The phenolic composition of the CA infusion was identified by reversed phase-high performance liquid chromatography-diode array detection (RP-HPCC-DAD).
31074673	1	56	theme	crypt	197:201	arg1	lesion					231:236	the precursor lesion	217:236	the precursor lesion of colorectal carcinogenesis (CRC), one of the most common malignancies in the world	217:321	Aberrant crypt foci (ACF) is the precursor lesion of colorectal carcinogenesis (CRC), one of the most common malignancies in the world.
31074673	1	56	theme	crypt	197:201	arg1	ACF					209:211	ACF	209:211	ACF	209:211	Aberrant crypt foci (ACF) is the precursor lesion of colorectal carcinogenesis (CRC), one of the most common malignancies in the world.
31074673	1	56	theme	crypt	197:201	arg1	foci					203:206	Aberrant crypt foci	188:206	Aberrant crypt foci (ACF)	188:212	Aberrant crypt foci (ACF) is the precursor lesion of colorectal carcinogenesis (CRC), one of the most common malignancies in the world.
31074673	10	57	from	stage	1866:1870	arg1	%					1846:1846	41.8%	1842:1846	41.8% at the subchronic stage	1842:1870	Our data suggest that CA treatment has preventive effects against AOM-/DSS-induced premalignant colon lesions in colon rats at the promotion level, inhibiting the cell proliferation of early neoplastic lesions and colonic inflammation through the decrease of β-catenin by 41.8% at the subchronic stage and 29% at the chronic stage, along with a 46.2% reduction of cyclooxygenase 2 (COX-2) at long term, despite a high expression of NF-κB (30.3% at the subchronic stage and 22.8% at the chronic stage).
31074673	10	57	from	stage	1866:1870	arg1	%					1878:1878	29%	1876:1878	29% at the chronic stage	1876:1899	Our data suggest that CA treatment has preventive effects against AOM-/DSS-induced premalignant colon lesions in colon rats at the promotion level, inhibiting the cell proliferation of early neoplastic lesions and colonic inflammation through the decrease of β-catenin by 41.8% at the subchronic stage and 29% at the chronic stage, along with a 46.2% reduction of cyclooxygenase 2 (COX-2) at long term, despite a high expression of NF-κB (30.3% at the subchronic stage and 22.8% at the chronic stage).
31074673	8	58	theme	chronic	1492:1498	arg1	stages					1500:1505	chronic stages	1492:1505	chronic stages	1492:1505	Eleven phenolic compounds were identified in the infusions, and ACF formation was reduced by 29.5% at the subchronic and by 64.6% at chronic stages.
31074673	7	59	theme	immunohistochemistry	1328:1347	arg1	analysis					1349:1356	immunohistochemistry analysis	1328:1356	immunohistochemistry analysis	1328:1356	Furthermore, 4 μm of colon, liver, and kidney was collected and stained with hematoxylin and eosin for histopathological analysis, along with 7 μm of colon for immunohistochemistry analysis.
31074673	10	60	from	stage	2064:2068	arg1	%					2047:2047	22.8%	2043:2047	22.8% at the chronic stage	2043:2068	Our data suggest that CA treatment has preventive effects against AOM-/DSS-induced premalignant colon lesions in colon rats at the promotion level, inhibiting the cell proliferation of early neoplastic lesions and colonic inflammation through the decrease of β-catenin by 41.8% at the subchronic stage and 29% at the chronic stage, along with a 46.2% reduction of cyclooxygenase 2 (COX-2) at long term, despite a high expression of NF-κB (30.3% at the subchronic stage and 22.8% at the chronic stage).
31074673	10	60	from	stage	2064:2068	arg1	%					2013:2013	30.3%	2009:2013	30.3% at the subchronic stage	2009:2037	Our data suggest that CA treatment has preventive effects against AOM-/DSS-induced premalignant colon lesions in colon rats at the promotion level, inhibiting the cell proliferation of early neoplastic lesions and colonic inflammation through the decrease of β-catenin by 41.8% at the subchronic stage and 29% at the chronic stage, along with a 46.2% reduction of cyclooxygenase 2 (COX-2) at long term, despite a high expression of NF-κB (30.3% at the subchronic stage and 22.8% at the chronic stage).
31074673	10	61	theme	cell	1733:1736	arg1	proliferation					1738:1750	the cell proliferation	1729:1750	the cell proliferation of early neoplastic lesions	1729:1778	Our data suggest that CA treatment has preventive effects against AOM-/DSS-induced premalignant colon lesions in colon rats at the promotion level, inhibiting the cell proliferation of early neoplastic lesions and colonic inflammation through the decrease of β-catenin by 41.8% at the subchronic stage and 29% at the chronic stage, along with a 46.2% reduction of cyclooxygenase 2 (COX-2) at long term, despite a high expression of NF-κB (30.3% at the subchronic stage and 22.8% at the chronic stage).
31074673	7	62	theme	colon	1318:1322	arg1	7 μm					1310:1313	7 μm	1310:1313	7 μm of colon for immunohistochemistry analysis	1310:1356	Furthermore, 4 μm of colon, liver, and kidney was collected and stained with hematoxylin and eosin for histopathological analysis, along with 7 μm of colon for immunohistochemistry analysis.
31074673	10	63	theme	subchronic	2022:2031	arg1	stage					2033:2037	the subchronic stage	2018:2037	the subchronic stage	2018:2037	Our data suggest that CA treatment has preventive effects against AOM-/DSS-induced premalignant colon lesions in colon rats at the promotion level, inhibiting the cell proliferation of early neoplastic lesions and colonic inflammation through the decrease of β-catenin by 41.8% at the subchronic stage and 29% at the chronic stage, along with a 46.2% reduction of cyclooxygenase 2 (COX-2) at long term, despite a high expression of NF-κB (30.3% at the subchronic stage and 22.8% at the chronic stage).
31074673	0	64	theme	Dextran	164:170	arg1	Sulfate					172:178	Dextran Sulfate	164:178	Dextran Sulfate	164:178	Preventive Effect of an Infusion of the Aqueous Extract of Chaya Leaves (Cnidoscolus aconitifolius) in an Aberrant Crypt Foci Rat Model Induced by Azoxymethane and Dextran Sulfate Sodium.
31074673	3	65	theme	potential	466:474	arg1	example					441:447	One example	437:447	One example of the botanical potential of preventive plant products	437:503	One example of the botanical potential of preventive plant products is Cnidoscolus aconitifolius (CA), commonly known as Chaya.
31074673	10	66	theme	%	1919:1919	arg1	reduction					1921:1929	a 46.2% reduction	1913:1929	a 46.2% reduction of cyclooxygenase 2 (COX-2) at long term	1913:1970	Our data suggest that CA treatment has preventive effects against AOM-/DSS-induced premalignant colon lesions in colon rats at the promotion level, inhibiting the cell proliferation of early neoplastic lesions and colonic inflammation through the decrease of β-catenin by 41.8% at the subchronic stage and 29% at the chronic stage, along with a 46.2% reduction of cyclooxygenase 2 (COX-2) at long term, despite a high expression of NF-κB (30.3% at the subchronic stage and 22.8% at the chronic stage).
31074673	10	67	theme	neoplastic	1761:1770	arg1	lesions					1772:1778	early neoplastic lesions	1755:1778	early neoplastic lesions	1755:1778	Our data suggest that CA treatment has preventive effects against AOM-/DSS-induced premalignant colon lesions in colon rats at the promotion level, inhibiting the cell proliferation of early neoplastic lesions and colonic inflammation through the decrease of β-catenin by 41.8% at the subchronic stage and 29% at the chronic stage, along with a 46.2% reduction of cyclooxygenase 2 (COX-2) at long term, despite a high expression of NF-κB (30.3% at the subchronic stage and 22.8% at the chronic stage).
31074673	2	68	with	people	356:361	arg1	intake					389:394	higher phytochemical intake	368:394	higher phytochemical intake	368:394	Many studies have reported that people with higher phytochemical intake are at a reduced risk of developing ACF.
31074673	4	69	theme	molecular	730:738	arg1	biomarkers					740:749	molecular biomarkers	730:749	molecular biomarkers	730:749	This study evaluated the phenolic profile of CA and the effects of the daily consumption of CA leaf infusion on the formation of ACF, histopathological lesions, and molecular biomarkers after azoxymethane (AOM) and dextran sulfate sodium (DSS) induced premalignant colon lesions in rats treated with for 16 and 32 weeks.
31074673	7	70	theme	histopathological	1271:1287	arg1	analysis					1289:1296	histopathological analysis	1271:1296	histopathological analysis	1271:1296	Furthermore, 4 μm of colon, liver, and kidney was collected and stained with hematoxylin and eosin for histopathological analysis, along with 7 μm of colon for immunohistochemistry analysis.
31074673	3	71	theme	products	496:503	arg1	potential					466:474	the botanical potential	452:474	the botanical potential of preventive plant products	452:503	One example of the botanical potential of preventive plant products is Cnidoscolus aconitifolius (CA), commonly known as Chaya.
31074673	3	72	theme	preventive	479:488	arg1	products					496:503	preventive plant products	479:503	preventive plant products	479:503	One example of the botanical potential of preventive plant products is Cnidoscolus aconitifolius (CA), commonly known as Chaya.
31074673	0	73	theme	Crypt	115:119	arg1	Model					130:134	an Aberrant Crypt Foci Rat Model	103:134	an Aberrant Crypt Foci Rat Model Induced by Azoxymethane and Dextran Sulfate Sodium	103:185	Preventive Effect of an Infusion of the Aqueous Extract of Chaya Leaves (Cnidoscolus aconitifolius) in an Aberrant Crypt Foci Rat Model Induced by Azoxymethane and Dextran Sulfate Sodium.
31074673	0	74	dep	Azoxymethane	147:158	arg1	Sodium					180:185	Sodium	180:185	Sodium	180:185	Preventive Effect of an Infusion of the Aqueous Extract of Chaya Leaves (Cnidoscolus aconitifolius) in an Aberrant Crypt Foci Rat Model Induced by Azoxymethane and Dextran Sulfate Sodium.
31074673	0	75	theme	Rat	126:128	arg1	Model					130:134	an Aberrant Crypt Foci Rat Model	103:134	an Aberrant Crypt Foci Rat Model Induced by Azoxymethane and Dextran Sulfate Sodium	103:185	Preventive Effect of an Infusion of the Aqueous Extract of Chaya Leaves (Cnidoscolus aconitifolius) in an Aberrant Crypt Foci Rat Model Induced by Azoxymethane and Dextran Sulfate Sodium.
31074673	5	76	theme	phase-high	957:966	arg1	RP-HPCC-DAD					1025:1035	RP-HPCC-DAD	1025:1035	RP-HPCC-DAD	1025:1035	The phenolic composition of the CA infusion was identified by reversed phase-high performance liquid chromatography-diode array detection (RP-HPCC-DAD).
31074673	5	76	theme	phase-high	957:966	arg1	detection					1014:1022	reversed phase-high performance liquid chromatography-diode array detection	948:1022	reversed phase-high performance liquid chromatography-diode array detection (RP-HPCC-DAD)	948:1036	The phenolic composition of the CA infusion was identified by reversed phase-high performance liquid chromatography-diode array detection (RP-HPCC-DAD).
31074673	3	77	theme	Cnidoscolus	508:518	arg1	CA					535:536	CA	535:536	CA	535:536	One example of the botanical potential of preventive plant products is Cnidoscolus aconitifolius (CA), commonly known as Chaya.
31074673	3	77	theme	Cnidoscolus	508:518	arg1	aconitifolius					520:532	Cnidoscolus aconitifolius	508:532	Cnidoscolus aconitifolius (CA)	508:537	One example of the botanical potential of preventive plant products is Cnidoscolus aconitifolius (CA), commonly known as Chaya.
31074673	4	78	theme	dextran	780:786	arg1	sodium					796:801	dextran sulfate sodium	780:801	dextran sulfate sodium (DSS)	780:807	This study evaluated the phenolic profile of CA and the effects of the daily consumption of CA leaf infusion on the formation of ACF, histopathological lesions, and molecular biomarkers after azoxymethane (AOM) and dextran sulfate sodium (DSS) induced premalignant colon lesions in rats treated with for 16 and 32 weeks.
31074673	4	78	theme	dextran	780:786	arg1	DSS					804:806	DSS	804:806	DSS	804:806	This study evaluated the phenolic profile of CA and the effects of the daily consumption of CA leaf infusion on the formation of ACF, histopathological lesions, and molecular biomarkers after azoxymethane (AOM) and dextran sulfate sodium (DSS) induced premalignant colon lesions in rats treated with for 16 and 32 weeks.
31074673	5	79	theme	CA	918:919	arg1	infusion					921:928	the CA infusion	914:928	the CA infusion	914:928	The phenolic composition of the CA infusion was identified by reversed phase-high performance liquid chromatography-diode array detection (RP-HPCC-DAD).
31074673	10	80	dep	NF-κB	2002:2006	arg1	%					2047:2047	22.8%	2043:2047	22.8% at the chronic stage	2043:2068	Our data suggest that CA treatment has preventive effects against AOM-/DSS-induced premalignant colon lesions in colon rats at the promotion level, inhibiting the cell proliferation of early neoplastic lesions and colonic inflammation through the decrease of β-catenin by 41.8% at the subchronic stage and 29% at the chronic stage, along with a 46.2% reduction of cyclooxygenase 2 (COX-2) at long term, despite a high expression of NF-κB (30.3% at the subchronic stage and 22.8% at the chronic stage).
31074673	10	80	dep	NF-κB	2002:2006	arg1	%					2013:2013	30.3%	2009:2013	30.3% at the subchronic stage	2009:2037	Our data suggest that CA treatment has preventive effects against AOM-/DSS-induced premalignant colon lesions in colon rats at the promotion level, inhibiting the cell proliferation of early neoplastic lesions and colonic inflammation through the decrease of β-catenin by 41.8% at the subchronic stage and 29% at the chronic stage, along with a 46.2% reduction of cyclooxygenase 2 (COX-2) at long term, despite a high expression of NF-κB (30.3% at the subchronic stage and 22.8% at the chronic stage).
31074673	7	81	theme	kidney	1207:1212	arg1	4 μm					1181:1184	4 μm	1181:1184	4 μm of colon, liver, and kidney	1181:1212	Furthermore, 4 μm of colon, liver, and kidney was collected and stained with hematoxylin and eosin for histopathological analysis, along with 7 μm of colon for immunohistochemistry analysis.
31074673	10	82	theme	premalignant	1653:1664	arg1	lesions					1672:1678	AOM-/DSS-induced premalignant colon lesions	1636:1678	AOM-/DSS-induced premalignant colon lesions	1636:1678	Our data suggest that CA treatment has preventive effects against AOM-/DSS-induced premalignant colon lesions in colon rats at the promotion level, inhibiting the cell proliferation of early neoplastic lesions and colonic inflammation through the decrease of β-catenin by 41.8% at the subchronic stage and 29% at the chronic stage, along with a 46.2% reduction of cyclooxygenase 2 (COX-2) at long term, despite a high expression of NF-κB (30.3% at the subchronic stage and 22.8% at the chronic stage).
31074673	2	83	theme	developing	421:430	arg1	ACF					432:434	developing ACF	421:434	developing ACF	421:434	Many studies have reported that people with higher phytochemical intake are at a reduced risk of developing ACF.
31074673	1	84	theme	common	290:295	arg1	malignancies					297:308	the most common malignancies	281:308	the most common malignancies in the world	281:321	Aberrant crypt foci (ACF) is the precursor lesion of colorectal carcinogenesis (CRC), one of the most common malignancies in the world.
31074673	4	85	theme	CA	610:611	arg1	profile					599:605	the phenolic profile	586:605	the phenolic profile of CA	586:611	This study evaluated the phenolic profile of CA and the effects of the daily consumption of CA leaf infusion on the formation of ACF, histopathological lesions, and molecular biomarkers after azoxymethane (AOM) and dextran sulfate sodium (DSS) induced premalignant colon lesions in rats treated with for 16 and 32 weeks.
31074673	4	86	theme	colon	830:834	arg1	lesions					836:842	premalignant colon lesions	817:842	premalignant colon lesions	817:842	This study evaluated the phenolic profile of CA and the effects of the daily consumption of CA leaf infusion on the formation of ACF, histopathological lesions, and molecular biomarkers after azoxymethane (AOM) and dextran sulfate sodium (DSS) induced premalignant colon lesions in rats treated with for 16 and 32 weeks.
31074673	10	87	theme	high	1983:1986	arg1	expression					1988:1997	a high expression	1981:1997	a high expression of NF-κB (30.3% at the subchronic stage and 22.8% at the chronic stage)	1981:2069	Our data suggest that CA treatment has preventive effects against AOM-/DSS-induced premalignant colon lesions in colon rats at the promotion level, inhibiting the cell proliferation of early neoplastic lesions and colonic inflammation through the decrease of β-catenin by 41.8% at the subchronic stage and 29% at the chronic stage, along with a 46.2% reduction of cyclooxygenase 2 (COX-2) at long term, despite a high expression of NF-κB (30.3% at the subchronic stage and 22.8% at the chronic stage).
31074673	4	88	theme	leaf	660:663	arg1	infusion					665:672	CA leaf infusion	657:672	CA leaf infusion	657:672	This study evaluated the phenolic profile of CA and the effects of the daily consumption of CA leaf infusion on the formation of ACF, histopathological lesions, and molecular biomarkers after azoxymethane (AOM) and dextran sulfate sodium (DSS) induced premalignant colon lesions in rats treated with for 16 and 32 weeks.
31074673	1	89	theme	malignancies	297:308	arg1	malignancies					297:308	the most common malignancies	281:308	the most common malignancies in the world	281:321	Aberrant crypt foci (ACF) is the precursor lesion of colorectal carcinogenesis (CRC), one of the most common malignancies in the world.
31074673	1	89	theme	malignancies	297:308	arg1	one					274:276	one	274:276	one	274:276	Aberrant crypt foci (ACF) is the precursor lesion of colorectal carcinogenesis (CRC), one of the most common malignancies in the world.
31074673	1	89	theme	malignancies	297:308	arg1	carcinogenesis					252:265	colorectal carcinogenesis	241:265	colorectal carcinogenesis (CRC)	241:271	Aberrant crypt foci (ACF) is the precursor lesion of colorectal carcinogenesis (CRC), one of the most common malignancies in the world.
31074673	7	90	theme	colon	1189:1193	arg1	4 μm					1181:1184	4 μm	1181:1184	4 μm of colon, liver, and kidney	1181:1212	Furthermore, 4 μm of colon, liver, and kidney was collected and stained with hematoxylin and eosin for histopathological analysis, along with 7 μm of colon for immunohistochemistry analysis.
31074673	2	91	theme	Many	324:327	arg1	studies					329:335	Many studies	324:335	Many studies	324:335	Many studies have reported that people with higher phytochemical intake are at a reduced risk of developing ACF.
31074673	5	92	theme	array	1008:1012	arg1	RP-HPCC-DAD					1025:1035	RP-HPCC-DAD	1025:1035	RP-HPCC-DAD	1025:1035	The phenolic composition of the CA infusion was identified by reversed phase-high performance liquid chromatography-diode array detection (RP-HPCC-DAD).
31074673	5	92	theme	array	1008:1012	arg1	detection					1014:1022	reversed phase-high performance liquid chromatography-diode array detection	948:1022	reversed phase-high performance liquid chromatography-diode array detection (RP-HPCC-DAD)	948:1036	The phenolic composition of the CA infusion was identified by reversed phase-high performance liquid chromatography-diode array detection (RP-HPCC-DAD).
31074673	5	93	theme	liquid	980:985	arg1	RP-HPCC-DAD					1025:1035	RP-HPCC-DAD	1025:1035	RP-HPCC-DAD	1025:1035	The phenolic composition of the CA infusion was identified by reversed phase-high performance liquid chromatography-diode array detection (RP-HPCC-DAD).
31074673	5	93	theme	liquid	980:985	arg1	detection					1014:1022	reversed phase-high performance liquid chromatography-diode array detection	948:1022	reversed phase-high performance liquid chromatography-diode array detection (RP-HPCC-DAD)	948:1036	The phenolic composition of the CA infusion was identified by reversed phase-high performance liquid chromatography-diode array detection (RP-HPCC-DAD).
31074673	2	94	theme	phytochemical	375:387	arg1	intake					389:394	higher phytochemical intake	368:394	higher phytochemical intake	368:394	Many studies have reported that people with higher phytochemical intake are at a reduced risk of developing ACF.
31074673	4	95	theme	daily	636:640	arg1	consumption					642:652	the daily consumption	632:652	the daily consumption of CA leaf infusion	632:672	This study evaluated the phenolic profile of CA and the effects of the daily consumption of CA leaf infusion on the formation of ACF, histopathological lesions, and molecular biomarkers after azoxymethane (AOM) and dextran sulfate sodium (DSS) induced premalignant colon lesions in rats treated with for 16 and 32 weeks.
31074673	10	96	theme	chronic	2056:2062	arg1	stage					2064:2068	the chronic stage	2052:2068	the chronic stage	2052:2068	Our data suggest that CA treatment has preventive effects against AOM-/DSS-induced premalignant colon lesions in colon rats at the promotion level, inhibiting the cell proliferation of early neoplastic lesions and colonic inflammation through the decrease of β-catenin by 41.8% at the subchronic stage and 29% at the chronic stage, along with a 46.2% reduction of cyclooxygenase 2 (COX-2) at long term, despite a high expression of NF-κB (30.3% at the subchronic stage and 22.8% at the chronic stage).
31074673	8	97	theme	ACF	1423:1425	arg1	formation					1427:1435	ACF formation	1423:1435	ACF formation	1423:1435	Eleven phenolic compounds were identified in the infusions, and ACF formation was reduced by 29.5% at the subchronic and by 64.6% at chronic stages.
31074673	1	98	theme	carcinogenesis	252:265	arg1	lesion					231:236	the precursor lesion	217:236	the precursor lesion of colorectal carcinogenesis (CRC), one of the most common malignancies in the world	217:321	Aberrant crypt foci (ACF) is the precursor lesion of colorectal carcinogenesis (CRC), one of the most common malignancies in the world.
31074673	1	98	theme	carcinogenesis	252:265	arg1	foci					203:206	Aberrant crypt foci	188:206	Aberrant crypt foci (ACF)	188:212	Aberrant crypt foci (ACF) is the precursor lesion of colorectal carcinogenesis (CRC), one of the most common malignancies in the world.
31074673	10	99	theme	colon	1683:1687	arg1	rats					1689:1692	colon rats	1683:1692	colon rats	1683:1692	Our data suggest that CA treatment has preventive effects against AOM-/DSS-induced premalignant colon lesions in colon rats at the promotion level, inhibiting the cell proliferation of early neoplastic lesions and colonic inflammation through the decrease of β-catenin by 41.8% at the subchronic stage and 29% at the chronic stage, along with a 46.2% reduction of cyclooxygenase 2 (COX-2) at long term, despite a high expression of NF-κB (30.3% at the subchronic stage and 22.8% at the chronic stage).
31074673	4	100	theme	histopathological	699:715	arg1	lesions					717:723	histopathological lesions	699:723	histopathological lesions	699:723	This study evaluated the phenolic profile of CA and the effects of the daily consumption of CA leaf infusion on the formation of ACF, histopathological lesions, and molecular biomarkers after azoxymethane (AOM) and dextran sulfate sodium (DSS) induced premalignant colon lesions in rats treated with for 16 and 32 weeks.
31074673	0	101	theme	Aqueous	40:46	arg1	Extract					48:54	the Aqueous Extract	36:54	the Aqueous Extract of Chaya	36:63	Preventive Effect of an Infusion of the Aqueous Extract of Chaya Leaves (Cnidoscolus aconitifolius) in an Aberrant Crypt Foci Rat Model Induced by Azoxymethane and Dextran Sulfate Sodium.
31074673	10	102	theme	promotion	1701:1709	arg1	level					1711:1715	the promotion level	1697:1715	the promotion level	1697:1715	Our data suggest that CA treatment has preventive effects against AOM-/DSS-induced premalignant colon lesions in colon rats at the promotion level, inhibiting the cell proliferation of early neoplastic lesions and colonic inflammation through the decrease of β-catenin by 41.8% at the subchronic stage and 29% at the chronic stage, along with a 46.2% reduction of cyclooxygenase 2 (COX-2) at long term, despite a high expression of NF-κB (30.3% at the subchronic stage and 22.8% at the chronic stage).
31074673	4	103	from	effects	621:627	arg1	formation					681:689	the formation	677:689	the formation of ACF, histopathological lesions, and molecular biomarkers after azoxymethane (AOM) and dextran sulfate sodium (DSS)	677:807	This study evaluated the phenolic profile of CA and the effects of the daily consumption of CA leaf infusion on the formation of ACF, histopathological lesions, and molecular biomarkers after azoxymethane (AOM) and dextran sulfate sodium (DSS) induced premalignant colon lesions in rats treated with for 16 and 32 weeks.
31074673	4	104	theme	ACF	694:696	arg1	formation					681:689	the formation	677:689	the formation of ACF, histopathological lesions, and molecular biomarkers after azoxymethane (AOM) and dextran sulfate sodium (DSS)	677:807	This study evaluated the phenolic profile of CA and the effects of the daily consumption of CA leaf infusion on the formation of ACF, histopathological lesions, and molecular biomarkers after azoxymethane (AOM) and dextran sulfate sodium (DSS) induced premalignant colon lesions in rats treated with for 16 and 32 weeks.
29914588	7	0	theme	Dry	1269:1271	arg1	DMI					1288:1290	DMI	1288:1290	DMI	1288:1290	Dry matter intake (DMI), milk yield, milk composition and methane production were measured at the end of each experimental period when cows were housed in respiration chambers for 4 days.
29914588	7	0	theme	Dry	1269:1271	arg1	intake					1280:1285	Dry matter intake	1269:1285	Dry matter intake (DMI)	1269:1291	Dry matter intake (DMI), milk yield, milk composition and methane production were measured at the end of each experimental period when cows were housed in respiration chambers for 4 days.
29914588	10	1	theme	fat	1962:1964	arg1	supplements					1966:1976	All the fat supplements	1954:1976	All the fat supplements	1954:1976	All the fat supplements decreased milk total saturated fatty acid (SFA) concentration compared with the control, and SFA were replaced with mainly cis-9 18:1 but also trans FA (and in the case of EL and CPLO there were increases in polyunsaturated FA concentration).
29914588	6	2	theme	palm	1120:1123	arg1	salts					1111:1115	calcium salts	1103:1115	calcium salts of palm and linseed oil (CPLO)	1103:1146	Dietary treatments were a control diet containing no supplemental fat, and three treatment diets containing extruded linseed (EL), calcium salts of palm and linseed oil (CPLO) or milled rapeseed (MR) formulated to provide each cow with an estimated 500 g additional oil/day (22 g oil/kg diet DM).
29914588	2	3	theme	effect	322:327	arg1	comparisons					303:313	direct comparisons	296:313	direct comparisons of the effect of different oil sources	296:352	However, direct comparisons of the effect of different oil sources are very few, as are studies implementing supplementation levels that reflect what is commonly fed on commercial farms.
29914588	6	4	theme	g	1250:1250	arg1	oil/day					1238:1244	an estimated 500 g additional oil/day	1208:1244	an estimated 500 g additional oil/day (22 g oil/kg diet DM)	1208:1266	Dietary treatments were a control diet containing no supplemental fat, and three treatment diets containing extruded linseed (EL), calcium salts of palm and linseed oil (CPLO) or milled rapeseed (MR) formulated to provide each cow with an estimated 500 g additional oil/day (22 g oil/kg diet DM).
29914588	6	4	theme	g	1250:1250	arg1	DM					1264:1265	22 g oil/kg diet DM	1247:1265	22 g oil/kg diet DM	1247:1265	Dietary treatments were a control diet containing no supplemental fat, and three treatment diets containing extruded linseed (EL), calcium salts of palm and linseed oil (CPLO) or milled rapeseed (MR) formulated to provide each cow with an estimated 500 g additional oil/day (22 g oil/kg diet DM).
29914588	11	5	theme	cow	2241:2243	arg1	diets					2245:2249	dairy cow diets	2235:2249	dairy cow diets	2235:2249	Supplementing dairy cow diets with these oilseed-based preparations affected milk FA profile and increased milk yield.
29914588	9	6	theme	2.7	1851:1853	arg1	l/kg					1855:1858	l/kg	1855:1858	l/kg	1855:1858	Feeding CPLO reduced methane production, and both linseed-based supplements decreased methane yield (by 1.8 l/kg DMI) and intensity (by 2.7 l/kg milk yield) compared with the control diet, but feeding MR had no effect on methane emission.
29914588	10	7	theme	total	1993:1997	arg1	concentration					2026:2038	milk total saturated fatty acid (SFA) concentration	1988:2038	milk total saturated fatty acid (SFA) concentration	1988:2038	All the fat supplements decreased milk total saturated fatty acid (SFA) concentration compared with the control, and SFA were replaced with mainly cis-9 18:1 but also trans FA (and in the case of EL and CPLO there were increases in polyunsaturated FA concentration).
29914588	6	8	theme	supplemental	1025:1036	arg1	fat					1038:1040	no supplemental fat	1022:1040	no supplemental fat	1022:1040	Dietary treatments were a control diet containing no supplemental fat, and three treatment diets containing extruded linseed (EL), calcium salts of palm and linseed oil (CPLO) or milled rapeseed (MR) formulated to provide each cow with an estimated 500 g additional oil/day (22 g oil/kg diet DM).
29914588	9	9	theme	milk	1860:1863	arg1	yield					1865:1869	2.7 l/kg milk yield	1851:1869	2.7 l/kg milk yield	1851:1869	Feeding CPLO reduced methane production, and both linseed-based supplements decreased methane yield (by 1.8 l/kg DMI) and intensity (by 2.7 l/kg milk yield) compared with the control diet, but feeding MR had no effect on methane emission.
29914588	5	10	with	ratio	870:874	arg1	the					905:907	the	905:907	the	905:907	Diets were fed as a total mixed ration with a 50 : 50 forage : concentrate ratio (dry matter (DM) basis) with the forage consisting of 75 : 25 maize silage : grass silage (DM).
29914588	8	11	theme	milk	1630:1633	arg1	fat					1635:1637	milk fat	1630:1637	milk fat concentration relative to control	1630:1671	There was no effect of treatment diet on DMI or milk protein or lactose concentration, but oilseed-based supplements increased milk yield compared with the control diet and milk fat concentration relative to control was reduced by 4 g/kg by supplemental EL.
29914588	3	12	theme	forms	539:543	arg1	effect					511:516	the effect	507:516	the effect of feeding different forms of supplemental plant oils on both methane emissions and milk fatty acid (FA) profile	507:629	The objective was to investigate the effect of feeding different forms of supplemental plant oils on both methane emissions and milk fatty acid (FA) profile.
29914588	10	13	dep	EL	2150:2151	arg1	were					2168:2171	were	2168:2171	were increases in polyunsaturated FA concentration	2168:2217	All the fat supplements decreased milk total saturated fatty acid (SFA) concentration compared with the control, and SFA were replaced with mainly cis-9 18:1 but also trans FA (and in the case of EL and CPLO there were increases in polyunsaturated FA concentration).
29914588	6	14	theme	control	998:1004	arg1	diet					1006:1009	a control diet	996:1009	a control diet containing no supplemental fat	996:1040	Dietary treatments were a control diet containing no supplemental fat, and three treatment diets containing extruded linseed (EL), calcium salts of palm and linseed oil (CPLO) or milled rapeseed (MR) formulated to provide each cow with an estimated 500 g additional oil/day (22 g oil/kg diet DM).
29914588	6	14	theme	control	998:1004	arg1	treatments					980:989	Dietary treatments	972:989	Dietary treatments	972:989	Dietary treatments were a control diet containing no supplemental fat, and three treatment diets containing extruded linseed (EL), calcium salts of palm and linseed oil (CPLO) or milled rapeseed (MR) formulated to provide each cow with an estimated 500 g additional oil/day (22 g oil/kg diet DM).
29914588	5	15	theme	maize	938:942	arg1	silage					944:949	75 : 25 maize silage	930:949	75 : 25 maize silage : grass silage (DM)	930:969	Diets were fed as a total mixed ration with a 50 : 50 forage : concentrate ratio (dry matter (DM) basis) with the forage consisting of 75 : 25 maize silage : grass silage (DM).
29914588	3	16	theme	feeding	521:527	arg1	forms					539:543	feeding different forms	521:543	feeding different forms of supplemental plant oils	521:570	The objective was to investigate the effect of feeding different forms of supplemental plant oils on both methane emissions and milk fatty acid (FA) profile.
29914588	8	17	theme	treatment	1480:1488	arg1	diet					1490:1493	treatment diet	1480:1493	treatment diet	1480:1493	There was no effect of treatment diet on DMI or milk protein or lactose concentration, but oilseed-based supplements increased milk yield compared with the control diet and milk fat concentration relative to control was reduced by 4 g/kg by supplemental EL.
29914588	7	18	theme	experimental	1379:1390	arg1	period					1392:1397	each experimental period	1374:1397	each experimental period	1374:1397	Dry matter intake (DMI), milk yield, milk composition and methane production were measured at the end of each experimental period when cows were housed in respiration chambers for 4 days.
29914588	10	19	theme	fatty	2009:2013	arg1	acid					2015:2018	saturated fatty acid	1999:2018	milk total saturated fatty acid (SFA) concentration	1988:2038	All the fat supplements decreased milk total saturated fatty acid (SFA) concentration compared with the control, and SFA were replaced with mainly cis-9 18:1 but also trans FA (and in the case of EL and CPLO there were increases in polyunsaturated FA concentration).
29914588	10	19	theme	fatty	2009:2013	arg1	SFA					2021:2023	SFA	2021:2023	SFA	2021:2023	All the fat supplements decreased milk total saturated fatty acid (SFA) concentration compared with the control, and SFA were replaced with mainly cis-9 18:1 but also trans FA (and in the case of EL and CPLO there were increases in polyunsaturated FA concentration).
29914588	9	20	theme	control	1890:1896	arg1	diet					1898:1901	the control diet	1886:1901	the control diet	1886:1901	Feeding CPLO reduced methane production, and both linseed-based supplements decreased methane yield (by 1.8 l/kg DMI) and intensity (by 2.7 l/kg milk yield) compared with the control diet, but feeding MR had no effect on methane emission.
29914588	3	21	theme	oils	567:570	arg1	forms					539:543	feeding different forms	521:543	feeding different forms of supplemental plant oils	521:570	The objective was to investigate the effect of feeding different forms of supplemental plant oils on both methane emissions and milk fatty acid (FA) profile.
29914588	3	22	theme	supplemental	548:559	arg1	oils					567:570	supplemental plant oils	548:570	supplemental plant oils	548:570	The objective was to investigate the effect of feeding different forms of supplemental plant oils on both methane emissions and milk fatty acid (FA) profile.
29914588	11	23	theme	milk	2328:2331	arg1	yield					2333:2337	increased milk yield	2318:2337	increased milk yield	2318:2337	Supplementing dairy cow diets with these oilseed-based preparations affected milk FA profile and increased milk yield.
29914588	5	24	theme	concentrate	858:868	arg1	basis					893:897	dry matter (DM) basis	877:897	dry matter (DM) basis	877:897	Diets were fed as a total mixed ration with a 50 : 50 forage : concentrate ratio (dry matter (DM) basis) with the forage consisting of 75 : 25 maize silage : grass silage (DM).
29914588	5	24	theme	concentrate	858:868	arg1	ratio					870:874	concentrate ratio	858:874	concentrate ratio (dry matter (DM) basis) with the forage consisting of 75 : 25 maize silage : grass silage (DM)	858:969	Diets were fed as a total mixed ration with a 50 : 50 forage : concentrate ratio (dry matter (DM) basis) with the forage consisting of 75 : 25 maize silage : grass silage (DM).
29914588	9	25	theme	feeding	1908:1914	arg1	MR					1916:1917	feeding MR	1908:1917	feeding MR	1908:1917	Feeding CPLO reduced methane production, and both linseed-based supplements decreased methane yield (by 1.8 l/kg DMI) and intensity (by 2.7 l/kg milk yield) compared with the control diet, but feeding MR had no effect on methane emission.
29914588	6	26	theme	linseed	1129:1135	arg1	CPLO					1142:1145	CPLO	1142:1145	CPLO	1142:1145	Dietary treatments were a control diet containing no supplemental fat, and three treatment diets containing extruded linseed (EL), calcium salts of palm and linseed oil (CPLO) or milled rapeseed (MR) formulated to provide each cow with an estimated 500 g additional oil/day (22 g oil/kg diet DM).
29914588	6	26	theme	linseed	1129:1135	arg1	oil					1137:1139	linseed oil	1129:1139	linseed oil (CPLO)	1129:1146	Dietary treatments were a control diet containing no supplemental fat, and three treatment diets containing extruded linseed (EL), calcium salts of palm and linseed oil (CPLO) or milled rapeseed (MR) formulated to provide each cow with an estimated 500 g additional oil/day (22 g oil/kg diet DM).
29914588	0	27	from	concentrations	86:99	arg1	cows					110:113	dairy cows	104:113	dairy cows	104:113	Differential effects of oilseed supplements on methane production and milk fatty acid concentrations in dairy cows.
29914588	9	28	theme	Feeding	1715:1721	arg1	CPLO					1723:1726	Feeding CPLO	1715:1726	Feeding CPLO	1715:1726	Feeding CPLO reduced methane production, and both linseed-based supplements decreased methane yield (by 1.8 l/kg DMI) and intensity (by 2.7 l/kg milk yield) compared with the control diet, but feeding MR had no effect on methane emission.
29914588	8	29	theme	lactose	1521:1527	arg1	concentration					1529:1541	lactose concentration	1521:1541	lactose concentration	1521:1541	There was no effect of treatment diet on DMI or milk protein or lactose concentration, but oilseed-based supplements increased milk yield compared with the control diet and milk fat concentration relative to control was reduced by 4 g/kg by supplemental EL.
29914588	5	30	theme	mixed	821:825	arg1	ration					827:832	a total mixed ration	813:832	a total mixed ration	813:832	Diets were fed as a total mixed ration with a 50 : 50 forage : concentrate ratio (dry matter (DM) basis) with the forage consisting of 75 : 25 maize silage : grass silage (DM).
29914588	5	30	theme	mixed	821:825	arg1	Diets					795:799	Diets	795:799	Diets	795:799	Diets were fed as a total mixed ration with a 50 : 50 forage : concentrate ratio (dry matter (DM) basis) with the forage consisting of 75 : 25 maize silage : grass silage (DM).
29914588	10	31	theme	polyunsaturated	2186:2200	arg1	concentration					2205:2217	polyunsaturated FA concentration	2186:2217	polyunsaturated FA concentration	2186:2217	All the fat supplements decreased milk total saturated fatty acid (SFA) concentration compared with the control, and SFA were replaced with mainly cis-9 18:1 but also trans FA (and in the case of EL and CPLO there were increases in polyunsaturated FA concentration).
29914588	0	32	theme	fatty	75:79	arg1	concentrations					86:99	milk fatty acid concentrations	70:99	milk fatty acid concentrations	70:99	Differential effects of oilseed supplements on methane production and milk fatty acid concentrations in dairy cows.
29914588	1	33	theme	cow	153:155	arg1	diets					157:161	dairy cow diets	147:161	dairy cow diets	147:161	It is known that supplementing dairy cow diets with full-fat oilseeds can be used as a strategy to mitigate methane emissions, through their action on rumen fermentation.
29914588	9	34	theme	methane	1801:1807	arg1	yield					1809:1813	methane yield	1801:1813	methane yield (by 1.8 l/kg DMI)	1801:1831	Feeding CPLO reduced methane production, and both linseed-based supplements decreased methane yield (by 1.8 l/kg DMI) and intensity (by 2.7 l/kg milk yield) compared with the control diet, but feeding MR had no effect on methane emission.
29914588	9	35	contain	had	1919:1921	arg1	MR					1916:1917	feeding MR	1908:1917	feeding MR	1908:1917	Feeding CPLO reduced methane production, and both linseed-based supplements decreased methane yield (by 1.8 l/kg DMI) and intensity (by 2.7 l/kg milk yield) compared with the control diet, but feeding MR had no effect on methane emission.
29914588	9	35	contain	had	1919:1921	arg2	effect					1926:1931	no effect	1923:1931	no effect	1923:1931	Feeding CPLO reduced methane production, and both linseed-based supplements decreased methane yield (by 1.8 l/kg DMI) and intensity (by 2.7 l/kg milk yield) compared with the control diet, but feeding MR had no effect on methane emission.
29914588	3	36	from	effect	511:516	arg1	profile					623:629	milk fatty acid (FA) profile	602:629	milk fatty acid (FA) profile	602:629	The objective was to investigate the effect of feeding different forms of supplemental plant oils on both methane emissions and milk fatty acid (FA) profile.
29914588	3	36	from	effect	511:516	arg1	emissions					588:596	methane emissions	580:596	methane emissions	580:596	The objective was to investigate the effect of feeding different forms of supplemental plant oils on both methane emissions and milk fatty acid (FA) profile.
29914588	9	37	theme	methane	1936:1942	arg1	emission					1944:1951	methane emission	1936:1951	methane emission	1936:1951	Feeding CPLO reduced methane production, and both linseed-based supplements decreased methane yield (by 1.8 l/kg DMI) and intensity (by 2.7 l/kg milk yield) compared with the control diet, but feeding MR had no effect on methane emission.
29914588	12	38	contain	had	2451:2453	arg2	effect					2458:2463	no effect	2455:2463	no effect	2455:2463	However, only the linseed-based supplements reduced methane production, yield or intensity, whereas feeding MR had no effect.
29914588	12	38	contain	had	2451:2453	arg1	MR					2448:2449	feeding MR	2440:2449	feeding MR	2440:2449	However, only the linseed-based supplements reduced methane production, yield or intensity, whereas feeding MR had no effect.
29914588	8	39	theme	oilseed-based	1548:1560	arg1	supplements					1562:1572	oilseed-based supplements	1548:1572	oilseed-based supplements	1548:1572	There was no effect of treatment diet on DMI or milk protein or lactose concentration, but oilseed-based supplements increased milk yield compared with the control diet and milk fat concentration relative to control was reduced by 4 g/kg by supplemental EL.
29914588	5	40	theme	dry	877:879	arg1	basis					893:897	dry matter (DM) basis	877:897	dry matter (DM) basis	877:897	Diets were fed as a total mixed ration with a 50 : 50 forage : concentrate ratio (dry matter (DM) basis) with the forage consisting of 75 : 25 maize silage : grass silage (DM).
29914588	5	40	theme	dry	877:879	arg1	ratio					870:874	concentrate ratio	858:874	concentrate ratio (dry matter (DM) basis) with the forage consisting of 75 : 25 maize silage : grass silage (DM)	858:969	Diets were fed as a total mixed ration with a 50 : 50 forage : concentrate ratio (dry matter (DM) basis) with the forage consisting of 75 : 25 maize silage : grass silage (DM).
29914588	7	41	theme	milk	1306:1309	arg1	composition					1311:1321	milk composition	1306:1321	milk composition	1306:1321	Dry matter intake (DMI), milk yield, milk composition and methane production were measured at the end of each experimental period when cows were housed in respiration chambers for 4 days.
29914588	4	42	theme	treatment	729:737	arg1	diets					739:743	four treatment diets	724:743	four treatment diets	724:743	Four multiparous, Holstein-Friesian cows in mid-lactation were randomly allocated to one of four treatment diets in a 4×4 Latin square design with 28-day periods.
29914588	0	43	theme	dairy	104:108	arg1	cows					110:113	dairy cows	104:113	dairy cows	104:113	Differential effects of oilseed supplements on methane production and milk fatty acid concentrations in dairy cows.
29914588	7	44	theme	respiration	1424:1434	arg1	chambers					1436:1443	respiration chambers	1424:1443	respiration chambers	1424:1443	Dry matter intake (DMI), milk yield, milk composition and methane production were measured at the end of each experimental period when cows were housed in respiration chambers for 4 days.
29914588	9	45	theme	linseed-based	1765:1777	arg1	supplements					1779:1789	both linseed-based supplements	1760:1789	both linseed-based supplements	1760:1789	Feeding CPLO reduced methane production, and both linseed-based supplements decreased methane yield (by 1.8 l/kg DMI) and intensity (by 2.7 l/kg milk yield) compared with the control diet, but feeding MR had no effect on methane emission.
29914588	0	46	theme	Differential	0:11	arg1	effects					13:19	Differential effects	0:19	Differential effects of oilseed supplements on methane production and milk fatty acid concentrations in dairy cows.	0:114	Differential effects of oilseed supplements on methane production and milk fatty acid concentrations in dairy cows.
29914588	2	47	theme	supplementation	396:410	arg1	levels					412:417	supplementation levels	396:417	supplementation levels that reflect what is commonly fed on commercial farms	396:471	However, direct comparisons of the effect of different oil sources are very few, as are studies implementing supplementation levels that reflect what is commonly fed on commercial farms.
29914588	11	48	theme	milk	2298:2301	arg1	profile					2306:2312	milk FA profile	2298:2312	milk FA profile	2298:2312	Supplementing dairy cow diets with these oilseed-based preparations affected milk FA profile and increased milk yield.
29914588	6	49	theme	g	1225:1225	arg1	oil/day					1238:1244	an estimated 500 g additional oil/day	1208:1244	an estimated 500 g additional oil/day (22 g oil/kg diet DM)	1208:1266	Dietary treatments were a control diet containing no supplemental fat, and three treatment diets containing extruded linseed (EL), calcium salts of palm and linseed oil (CPLO) or milled rapeseed (MR) formulated to provide each cow with an estimated 500 g additional oil/day (22 g oil/kg diet DM).
29914588	6	49	theme	g	1225:1225	arg1	DM					1264:1265	22 g oil/kg diet DM	1247:1265	22 g oil/kg diet DM	1247:1265	Dietary treatments were a control diet containing no supplemental fat, and three treatment diets containing extruded linseed (EL), calcium salts of palm and linseed oil (CPLO) or milled rapeseed (MR) formulated to provide each cow with an estimated 500 g additional oil/day (22 g oil/kg diet DM).
29914588	7	50	theme	milk	1294:1297	arg1	yield					1299:1303	milk yield	1294:1303	milk yield	1294:1303	Dry matter intake (DMI), milk yield, milk composition and methane production were measured at the end of each experimental period when cows were housed in respiration chambers for 4 days.
29914588	3	51	theme	acid	613:616	arg1	profile					623:629	milk fatty acid (FA) profile	602:629	milk fatty acid (FA) profile	602:629	The objective was to investigate the effect of feeding different forms of supplemental plant oils on both methane emissions and milk fatty acid (FA) profile.
29914588	4	52	theme	square	760:765	arg1	design					767:772	a 4×4 Latin square design	748:772	a 4×4 Latin square design with 28-day periods	748:792	Four multiparous, Holstein-Friesian cows in mid-lactation were randomly allocated to one of four treatment diets in a 4×4 Latin square design with 28-day periods.
29914588	0	53	theme	supplements	32:42	arg1	effects					13:19	Differential effects	0:19	Differential effects of oilseed supplements on methane production and milk fatty acid concentrations in dairy cows.	0:114	Differential effects of oilseed supplements on methane production and milk fatty acid concentrations in dairy cows.
29914588	6	54	theme	estimated	1211:1219	arg1	oil/day					1238:1244	an estimated 500 g additional oil/day	1208:1244	an estimated 500 g additional oil/day (22 g oil/kg diet DM)	1208:1266	Dietary treatments were a control diet containing no supplemental fat, and three treatment diets containing extruded linseed (EL), calcium salts of palm and linseed oil (CPLO) or milled rapeseed (MR) formulated to provide each cow with an estimated 500 g additional oil/day (22 g oil/kg diet DM).
29914588	6	54	theme	estimated	1211:1219	arg1	DM					1264:1265	22 g oil/kg diet DM	1247:1265	22 g oil/kg diet DM	1247:1265	Dietary treatments were a control diet containing no supplemental fat, and three treatment diets containing extruded linseed (EL), calcium salts of palm and linseed oil (CPLO) or milled rapeseed (MR) formulated to provide each cow with an estimated 500 g additional oil/day (22 g oil/kg diet DM).
29914588	11	55	theme	increased	2318:2326	arg1	yield					2333:2337	increased milk yield	2318:2337	increased milk yield	2318:2337	Supplementing dairy cow diets with these oilseed-based preparations affected milk FA profile and increased milk yield.
29914588	3	56	theme	milk	602:605	arg1	FA					619:620	FA	619:620	FA	619:620	The objective was to investigate the effect of feeding different forms of supplemental plant oils on both methane emissions and milk fatty acid (FA) profile.
29914588	3	56	theme	milk	602:605	arg1	acid					613:616	milk fatty acid	602:616	milk fatty acid (FA) profile	602:629	The objective was to investigate the effect of feeding different forms of supplemental plant oils on both methane emissions and milk fatty acid (FA) profile.
29914588	0	57	theme	methane	47:53	arg1	production					55:64	methane production	47:64	methane production	47:64	Differential effects of oilseed supplements on methane production and milk fatty acid concentrations in dairy cows.
29914588	10	58	theme	milk	1988:1991	arg1	concentration					2026:2038	milk total saturated fatty acid (SFA) concentration	1988:2038	milk total saturated fatty acid (SFA) concentration	1988:2038	All the fat supplements decreased milk total saturated fatty acid (SFA) concentration compared with the control, and SFA were replaced with mainly cis-9 18:1 but also trans FA (and in the case of EL and CPLO there were increases in polyunsaturated FA concentration).
29914588	9	59	theme	l/kg	1823:1826	arg1	DMI					1828:1830	1.8 l/kg DMI	1819:1830	1.8 l/kg DMI	1819:1830	Feeding CPLO reduced methane production, and both linseed-based supplements decreased methane yield (by 1.8 l/kg DMI) and intensity (by 2.7 l/kg milk yield) compared with the control diet, but feeding MR had no effect on methane emission.
29914588	7	60	theme	matter	1273:1278	arg1	DMI					1288:1290	DMI	1288:1290	DMI	1288:1290	Dry matter intake (DMI), milk yield, milk composition and methane production were measured at the end of each experimental period when cows were housed in respiration chambers for 4 days.
29914588	7	60	theme	matter	1273:1278	arg1	intake					1280:1285	Dry matter intake	1269:1285	Dry matter intake (DMI)	1269:1291	Dry matter intake (DMI), milk yield, milk composition and methane production were measured at the end of each experimental period when cows were housed in respiration chambers for 4 days.
29914588	11	61	theme	dairy	2235:2239	arg1	diets					2245:2249	dairy cow diets	2235:2249	dairy cow diets	2235:2249	Supplementing dairy cow diets with these oilseed-based preparations affected milk FA profile and increased milk yield.
29914588	6	62	theme	additional	1227:1236	arg1	g					1225:1225	500 g additional	1221:1236	an estimated 500 g additional oil/day (22 g oil/kg diet DM)	1208:1266	Dietary treatments were a control diet containing no supplemental fat, and three treatment diets containing extruded linseed (EL), calcium salts of palm and linseed oil (CPLO) or milled rapeseed (MR) formulated to provide each cow with an estimated 500 g additional oil/day (22 g oil/kg diet DM).
29914588	4	63	theme	4×4	750:752	arg1	design					767:772	a 4×4 Latin square design	748:772	a 4×4 Latin square design with 28-day periods	748:792	Four multiparous, Holstein-Friesian cows in mid-lactation were randomly allocated to one of four treatment diets in a 4×4 Latin square design with 28-day periods.
29914588	2	64	theme	oil	342:344	arg1	sources					346:352	different oil sources	332:352	different oil sources	332:352	However, direct comparisons of the effect of different oil sources are very few, as are studies implementing supplementation levels that reflect what is commonly fed on commercial farms.
29914588	5	65	dep	silage	944:949	arg1	DM					967:968	DM	967:968	DM	967:968	Diets were fed as a total mixed ration with a 50 : 50 forage : concentrate ratio (dry matter (DM) basis) with the forage consisting of 75 : 25 maize silage : grass silage (DM).
29914588	5	65	dep	silage	944:949	arg1	silage					959:964	grass silage	953:964	75 : 25 maize silage : grass silage (DM)	930:969	Diets were fed as a total mixed ration with a 50 : 50 forage : concentrate ratio (dry matter (DM) basis) with the forage consisting of 75 : 25 maize silage : grass silage (DM).
29914588	6	66	theme	oil/kg	1252:1257	arg1	oil/day					1238:1244	an estimated 500 g additional oil/day	1208:1244	an estimated 500 g additional oil/day (22 g oil/kg diet DM)	1208:1266	Dietary treatments were a control diet containing no supplemental fat, and three treatment diets containing extruded linseed (EL), calcium salts of palm and linseed oil (CPLO) or milled rapeseed (MR) formulated to provide each cow with an estimated 500 g additional oil/day (22 g oil/kg diet DM).
29914588	6	66	theme	oil/kg	1252:1257	arg1	DM					1264:1265	22 g oil/kg diet DM	1247:1265	22 g oil/kg diet DM	1247:1265	Dietary treatments were a control diet containing no supplemental fat, and three treatment diets containing extruded linseed (EL), calcium salts of palm and linseed oil (CPLO) or milled rapeseed (MR) formulated to provide each cow with an estimated 500 g additional oil/day (22 g oil/kg diet DM).
29914588	1	67	theme	methane	224:230	arg1	emissions					232:240	methane emissions	224:240	methane emissions	224:240	It is known that supplementing dairy cow diets with full-fat oilseeds can be used as a strategy to mitigate methane emissions, through their action on rumen fermentation.
29914588	12	68	theme	linseed-based	2358:2370	arg1	supplements					2372:2382	only the linseed-based supplements	2349:2382	only the linseed-based supplements	2349:2382	However, only the linseed-based supplements reduced methane production, yield or intensity, whereas feeding MR had no effect.
29914588	8	69	theme	control	1613:1619	arg1	diet					1621:1624	the control diet	1609:1624	the control diet	1609:1624	There was no effect of treatment diet on DMI or milk protein or lactose concentration, but oilseed-based supplements increased milk yield compared with the control diet and milk fat concentration relative to control was reduced by 4 g/kg by supplemental EL.
29914588	6	70	contain	containing	1011:1020	arg1	diet					1006:1009	a control diet	996:1009	a control diet containing no supplemental fat	996:1040	Dietary treatments were a control diet containing no supplemental fat, and three treatment diets containing extruded linseed (EL), calcium salts of palm and linseed oil (CPLO) or milled rapeseed (MR) formulated to provide each cow with an estimated 500 g additional oil/day (22 g oil/kg diet DM).
29914588	6	70	contain	containing	1011:1020	arg2	fat					1038:1040	no supplemental fat	1022:1040	no supplemental fat	1022:1040	Dietary treatments were a control diet containing no supplemental fat, and three treatment diets containing extruded linseed (EL), calcium salts of palm and linseed oil (CPLO) or milled rapeseed (MR) formulated to provide each cow with an estimated 500 g additional oil/day (22 g oil/kg diet DM).
29914588	6	70	contain	containing	1011:1020	arg1	treatments					980:989	Dietary treatments	972:989	Dietary treatments	972:989	Dietary treatments were a control diet containing no supplemental fat, and three treatment diets containing extruded linseed (EL), calcium salts of palm and linseed oil (CPLO) or milled rapeseed (MR) formulated to provide each cow with an estimated 500 g additional oil/day (22 g oil/kg diet DM).
29914588	9	71	theme	l/kg	1855:1858	arg1	yield					1865:1869	2.7 l/kg milk yield	1851:1869	2.7 l/kg milk yield	1851:1869	Feeding CPLO reduced methane production, and both linseed-based supplements decreased methane yield (by 1.8 l/kg DMI) and intensity (by 2.7 l/kg milk yield) compared with the control diet, but feeding MR had no effect on methane emission.
29914588	6	72	theme	calcium	1103:1109	arg1	salts					1111:1115	calcium salts	1103:1115	calcium salts of palm and linseed oil (CPLO)	1103:1146	Dietary treatments were a control diet containing no supplemental fat, and three treatment diets containing extruded linseed (EL), calcium salts of palm and linseed oil (CPLO) or milled rapeseed (MR) formulated to provide each cow with an estimated 500 g additional oil/day (22 g oil/kg diet DM).
29914588	4	73	theme	28-day	779:784	arg1	periods					786:792	28-day periods	779:792	28-day periods	779:792	Four multiparous, Holstein-Friesian cows in mid-lactation were randomly allocated to one of four treatment diets in a 4×4 Latin square design with 28-day periods.
29914588	8	74	theme	supplemental	1698:1709	arg1	EL					1711:1712	supplemental EL	1698:1712	supplemental EL	1698:1712	There was no effect of treatment diet on DMI or milk protein or lactose concentration, but oilseed-based supplements increased milk yield compared with the control diet and milk fat concentration relative to control was reduced by 4 g/kg by supplemental EL.
29914588	8	75	theme	fat	1635:1637	arg1	concentration					1639:1651	milk fat concentration	1630:1651	milk fat concentration relative to control	1630:1671	There was no effect of treatment diet on DMI or milk protein or lactose concentration, but oilseed-based supplements increased milk yield compared with the control diet and milk fat concentration relative to control was reduced by 4 g/kg by supplemental EL.
29914588	3	76	theme	different	529:537	arg1	forms					539:543	feeding different forms	521:543	feeding different forms of supplemental plant oils	521:570	The objective was to investigate the effect of feeding different forms of supplemental plant oils on both methane emissions and milk fatty acid (FA) profile.
29914588	1	77	theme	full-fat	168:175	arg1	oilseeds					177:184	full-fat oilseeds	168:184	full-fat oilseeds	168:184	It is known that supplementing dairy cow diets with full-fat oilseeds can be used as a strategy to mitigate methane emissions, through their action on rumen fermentation.
29914588	7	78	theme	period	1392:1397	arg1	end					1367:1369	the end	1363:1369	the end of each experimental period	1363:1397	Dry matter intake (DMI), milk yield, milk composition and methane production were measured at the end of each experimental period when cows were housed in respiration chambers for 4 days.
29914588	1	79	from	action	257:262	arg1	fermentation					273:284	rumen fermentation	267:284	rumen fermentation	267:284	It is known that supplementing dairy cow diets with full-fat oilseeds can be used as a strategy to mitigate methane emissions, through their action on rumen fermentation.
29914588	5	80	dep	the	905:907	arg1	forage					909:914	forage	909:914	forage	909:914	Diets were fed as a total mixed ration with a 50 : 50 forage : concentrate ratio (dry matter (DM) basis) with the forage consisting of 75 : 25 maize silage : grass silage (DM).
29914588	12	81	theme	methane	2392:2398	arg1	production					2400:2409	methane production	2392:2409	methane production	2392:2409	However, only the linseed-based supplements reduced methane production, yield or intensity, whereas feeding MR had no effect.
29914588	8	82	theme	relative	1653:1660	arg1	concentration					1639:1651	milk fat concentration	1630:1651	milk fat concentration relative to control	1630:1671	There was no effect of treatment diet on DMI or milk protein or lactose concentration, but oilseed-based supplements increased milk yield compared with the control diet and milk fat concentration relative to control was reduced by 4 g/kg by supplemental EL.
29914588	10	83	theme	acid	2015:2018	arg1	concentration					2026:2038	milk total saturated fatty acid (SFA) concentration	1988:2038	milk total saturated fatty acid (SFA) concentration	1988:2038	All the fat supplements decreased milk total saturated fatty acid (SFA) concentration compared with the control, and SFA were replaced with mainly cis-9 18:1 but also trans FA (and in the case of EL and CPLO there were increases in polyunsaturated FA concentration).
29914588	10	84	theme	EL	2150:2151	arg1	case					2142:2145	the case	2138:2145	the case of EL and CPLO there were increases in polyunsaturated FA concentration	2138:2217	All the fat supplements decreased milk total saturated fatty acid (SFA) concentration compared with the control, and SFA were replaced with mainly cis-9 18:1 but also trans FA (and in the case of EL and CPLO there were increases in polyunsaturated FA concentration).
29914588	3	85	theme	plant	561:565	arg1	oils					567:570	supplemental plant oils	548:570	supplemental plant oils	548:570	The objective was to investigate the effect of feeding different forms of supplemental plant oils on both methane emissions and milk fatty acid (FA) profile.
29914588	10	86	theme	saturated	1999:2007	arg1	acid					2015:2018	saturated fatty acid	1999:2018	milk total saturated fatty acid (SFA) concentration	1988:2038	All the fat supplements decreased milk total saturated fatty acid (SFA) concentration compared with the control, and SFA were replaced with mainly cis-9 18:1 but also trans FA (and in the case of EL and CPLO there were increases in polyunsaturated FA concentration).
29914588	10	86	theme	saturated	1999:2007	arg1	SFA					2021:2023	SFA	2021:2023	SFA	2021:2023	All the fat supplements decreased milk total saturated fatty acid (SFA) concentration compared with the control, and SFA were replaced with mainly cis-9 18:1 but also trans FA (and in the case of EL and CPLO there were increases in polyunsaturated FA concentration).
29914588	5	87	theme	grass	953:957	arg1	DM					967:968	DM	967:968	DM	967:968	Diets were fed as a total mixed ration with a 50 : 50 forage : concentrate ratio (dry matter (DM) basis) with the forage consisting of 75 : 25 maize silage : grass silage (DM).
29914588	5	87	theme	grass	953:957	arg1	silage					959:964	grass silage	953:964	75 : 25 maize silage : grass silage (DM)	930:969	Diets were fed as a total mixed ration with a 50 : 50 forage : concentrate ratio (dry matter (DM) basis) with the forage consisting of 75 : 25 maize silage : grass silage (DM).
29914588	8	88	theme	diet	1490:1493	arg1	effect					1470:1475	no effect	1467:1475	no effect of treatment diet on DMI or milk protein or lactose concentration	1467:1541	There was no effect of treatment diet on DMI or milk protein or lactose concentration, but oilseed-based supplements increased milk yield compared with the control diet and milk fat concentration relative to control was reduced by 4 g/kg by supplemental EL.
29914588	6	89	theme	oil	1137:1139	arg1	salts					1111:1115	calcium salts	1103:1115	calcium salts of palm and linseed oil (CPLO)	1103:1146	Dietary treatments were a control diet containing no supplemental fat, and three treatment diets containing extruded linseed (EL), calcium salts of palm and linseed oil (CPLO) or milled rapeseed (MR) formulated to provide each cow with an estimated 500 g additional oil/day (22 g oil/kg diet DM).
29914588	3	90	theme	methane	580:586	arg1	emissions					588:596	methane emissions	580:596	methane emissions	580:596	The objective was to investigate the effect of feeding different forms of supplemental plant oils on both methane emissions and milk fatty acid (FA) profile.
29914588	6	91	theme	treatment	1053:1061	arg1	diets					1063:1067	three treatment diets	1047:1067	three treatment diets containing extruded linseed (EL), calcium salts of palm and linseed oil (CPLO) or milled rapeseed (MR) formulated to provide each cow with an estimated 500 g additional oil/day (22 g oil/kg diet DM)	1047:1266	Dietary treatments were a control diet containing no supplemental fat, and three treatment diets containing extruded linseed (EL), calcium salts of palm and linseed oil (CPLO) or milled rapeseed (MR) formulated to provide each cow with an estimated 500 g additional oil/day (22 g oil/kg diet DM).
29914588	10	92	theme	CPLO	2157:2160	arg1	case					2142:2145	the case	2138:2145	the case of EL and CPLO there were increases in polyunsaturated FA concentration	2138:2217	All the fat supplements decreased milk total saturated fatty acid (SFA) concentration compared with the control, and SFA were replaced with mainly cis-9 18:1 but also trans FA (and in the case of EL and CPLO there were increases in polyunsaturated FA concentration).
29914588	4	93	with	design	767:772	arg1	periods					786:792	28-day periods	779:792	28-day periods	779:792	Four multiparous, Holstein-Friesian cows in mid-lactation were randomly allocated to one of four treatment diets in a 4×4 Latin square design with 28-day periods.
29914588	0	94	theme	milk	70:73	arg1	concentrations					86:99	milk fatty acid concentrations	70:99	milk fatty acid concentrations	70:99	Differential effects of oilseed supplements on methane production and milk fatty acid concentrations in dairy cows.
29914588	8	95	theme	milk	1505:1508	arg1	protein					1510:1516	milk protein	1505:1516	milk protein	1505:1516	There was no effect of treatment diet on DMI or milk protein or lactose concentration, but oilseed-based supplements increased milk yield compared with the control diet and milk fat concentration relative to control was reduced by 4 g/kg by supplemental EL.
29914588	5	96	theme	total	815:819	arg1	ration					827:832	a total mixed ration	813:832	a total mixed ration	813:832	Diets were fed as a total mixed ration with a 50 : 50 forage : concentrate ratio (dry matter (DM) basis) with the forage consisting of 75 : 25 maize silage : grass silage (DM).
29914588	5	96	theme	total	815:819	arg1	Diets					795:799	Diets	795:799	Diets	795:799	Diets were fed as a total mixed ration with a 50 : 50 forage : concentrate ratio (dry matter (DM) basis) with the forage consisting of 75 : 25 maize silage : grass silage (DM).
29914588	7	97	theme	methane	1327:1333	arg1	production					1335:1344	methane production	1327:1344	methane production	1327:1344	Dry matter intake (DMI), milk yield, milk composition and methane production were measured at the end of each experimental period when cows were housed in respiration chambers for 4 days.
29914588	2	98	theme	commercial	456:465	arg1	farms					467:471	commercial farms	456:471	commercial farms	456:471	However, direct comparisons of the effect of different oil sources are very few, as are studies implementing supplementation levels that reflect what is commonly fed on commercial farms.
29914588	10	99	theme	FA	2202:2203	arg1	concentration					2205:2217	polyunsaturated FA concentration	2186:2217	polyunsaturated FA concentration	2186:2217	All the fat supplements decreased milk total saturated fatty acid (SFA) concentration compared with the control, and SFA were replaced with mainly cis-9 18:1 but also trans FA (and in the case of EL and CPLO there were increases in polyunsaturated FA concentration).
29914588	0	100	theme	acid	81:84	arg1	concentrations					86:99	milk fatty acid concentrations	70:99	milk fatty acid concentrations	70:99	Differential effects of oilseed supplements on methane production and milk fatty acid concentrations in dairy cows.
29914588	1	101	theme	dairy	147:151	arg1	diets					157:161	dairy cow diets	147:161	dairy cow diets	147:161	It is known that supplementing dairy cow diets with full-fat oilseeds can be used as a strategy to mitigate methane emissions, through their action on rumen fermentation.
29914588	0	102	from	effects	13:19	arg1	concentrations					86:99	milk fatty acid concentrations	70:99	milk fatty acid concentrations	70:99	Differential effects of oilseed supplements on methane production and milk fatty acid concentrations in dairy cows.
29914588	0	102	from	effects	13:19	arg1	production					55:64	methane production	47:64	methane production	47:64	Differential effects of oilseed supplements on methane production and milk fatty acid concentrations in dairy cows.
29914588	9	103	theme	methane	1736:1742	arg1	production					1744:1753	methane production	1736:1753	methane production	1736:1753	Feeding CPLO reduced methane production, and both linseed-based supplements decreased methane yield (by 1.8 l/kg DMI) and intensity (by 2.7 l/kg milk yield) compared with the control diet, but feeding MR had no effect on methane emission.
29914588	4	104	theme	multiparous	637:647	arg1	cows					668:671	Four multiparous, Holstein-Friesian cows	632:671	cows	668:671	Four multiparous, Holstein-Friesian cows in mid-lactation were randomly allocated to one of four treatment diets in a 4×4 Latin square design with 28-day periods.
29914588	0	105	from	production	55:64	arg1	cows					110:113	dairy cows	104:113	dairy cows	104:113	Differential effects of oilseed supplements on methane production and milk fatty acid concentrations in dairy cows.
29914588	10	106	from	increases	2173:2181	arg1	concentration					2205:2217	polyunsaturated FA concentration	2186:2217	polyunsaturated FA concentration	2186:2217	All the fat supplements decreased milk total saturated fatty acid (SFA) concentration compared with the control, and SFA were replaced with mainly cis-9 18:1 but also trans FA (and in the case of EL and CPLO there were increases in polyunsaturated FA concentration).
29914588	5	107	dep	50	846:847	arg1	forage					849:854	forage	849:854	forage	849:854	Diets were fed as a total mixed ration with a 50 : 50 forage : concentrate ratio (dry matter (DM) basis) with the forage consisting of 75 : 25 maize silage : grass silage (DM).
29914588	5	108	theme	matter	881:886	arg1	basis					893:897	dry matter (DM) basis	877:897	dry matter (DM) basis	877:897	Diets were fed as a total mixed ration with a 50 : 50 forage : concentrate ratio (dry matter (DM) basis) with the forage consisting of 75 : 25 maize silage : grass silage (DM).
29914588	5	108	theme	matter	881:886	arg1	ratio					870:874	concentrate ratio	858:874	concentrate ratio (dry matter (DM) basis) with the forage consisting of 75 : 25 maize silage : grass silage (DM)	858:969	Diets were fed as a total mixed ration with a 50 : 50 forage : concentrate ratio (dry matter (DM) basis) with the forage consisting of 75 : 25 maize silage : grass silage (DM).
29914588	10	109	theme	cis-9	2101:2105	arg1	18:1					2107:2110	cis-9 18:1	2101:2110	cis-9 18:1 but also trans FA	2101:2128	All the fat supplements decreased milk total saturated fatty acid (SFA) concentration compared with the control, and SFA were replaced with mainly cis-9 18:1 but also trans FA (and in the case of EL and CPLO there were increases in polyunsaturated FA concentration).
29914588	6	110	contain	containing	1069:1078	arg2	linseed					1089:1095	linseed	1089:1095	linseed	1089:1095	Dietary treatments were a control diet containing no supplemental fat, and three treatment diets containing extruded linseed (EL), calcium salts of palm and linseed oil (CPLO) or milled rapeseed (MR) formulated to provide each cow with an estimated 500 g additional oil/day (22 g oil/kg diet DM).
29914588	6	110	contain	containing	1069:1078	arg2	salts					1111:1115	calcium salts	1103:1115	calcium salts of palm and linseed oil (CPLO)	1103:1146	Dietary treatments were a control diet containing no supplemental fat, and three treatment diets containing extruded linseed (EL), calcium salts of palm and linseed oil (CPLO) or milled rapeseed (MR) formulated to provide each cow with an estimated 500 g additional oil/day (22 g oil/kg diet DM).
29914588	6	110	contain	containing	1069:1078	arg2	MR					1168:1169	MR	1168:1169	MR	1168:1169	Dietary treatments were a control diet containing no supplemental fat, and three treatment diets containing extruded linseed (EL), calcium salts of palm and linseed oil (CPLO) or milled rapeseed (MR) formulated to provide each cow with an estimated 500 g additional oil/day (22 g oil/kg diet DM).
29914588	6	110	contain	containing	1069:1078	arg2	rapeseed					1158:1165	rapeseed	1158:1165	rapeseed	1158:1165	Dietary treatments were a control diet containing no supplemental fat, and three treatment diets containing extruded linseed (EL), calcium salts of palm and linseed oil (CPLO) or milled rapeseed (MR) formulated to provide each cow with an estimated 500 g additional oil/day (22 g oil/kg diet DM).
29914588	6	110	contain	containing	1069:1078	arg1	diets					1063:1067	three treatment diets	1047:1067	three treatment diets containing extruded linseed (EL), calcium salts of palm and linseed oil (CPLO) or milled rapeseed (MR) formulated to provide each cow with an estimated 500 g additional oil/day (22 g oil/kg diet DM)	1047:1266	Dietary treatments were a control diet containing no supplemental fat, and three treatment diets containing extruded linseed (EL), calcium salts of palm and linseed oil (CPLO) or milled rapeseed (MR) formulated to provide each cow with an estimated 500 g additional oil/day (22 g oil/kg diet DM).
29914588	6	110	contain	containing	1069:1078	arg2	EL					1098:1099	EL	1098:1099	EL	1098:1099	Dietary treatments were a control diet containing no supplemental fat, and three treatment diets containing extruded linseed (EL), calcium salts of palm and linseed oil (CPLO) or milled rapeseed (MR) formulated to provide each cow with an estimated 500 g additional oil/day (22 g oil/kg diet DM).
29914588	2	111	theme	sources	346:352	arg1	effect					322:327	the effect	318:327	the effect of different oil sources	318:352	However, direct comparisons of the effect of different oil sources are very few, as are studies implementing supplementation levels that reflect what is commonly fed on commercial farms.
29914588	0	112	theme	oilseed	24:30	arg1	supplements					32:42	oilseed supplements	24:42	oilseed supplements	24:42	Differential effects of oilseed supplements on methane production and milk fatty acid concentrations in dairy cows.
29914588	8	113	from	effect	1470:1475	arg1	DMI					1498:1500	DMI	1498:1500	DMI	1498:1500	There was no effect of treatment diet on DMI or milk protein or lactose concentration, but oilseed-based supplements increased milk yield compared with the control diet and milk fat concentration relative to control was reduced by 4 g/kg by supplemental EL.
29914588	8	113	from	effect	1470:1475	arg1	protein					1510:1516	milk protein	1505:1516	milk protein	1505:1516	There was no effect of treatment diet on DMI or milk protein or lactose concentration, but oilseed-based supplements increased milk yield compared with the control diet and milk fat concentration relative to control was reduced by 4 g/kg by supplemental EL.
29914588	8	114	theme	milk	1584:1587	arg1	yield					1589:1593	milk yield	1584:1593	milk yield	1584:1593	There was no effect of treatment diet on DMI or milk protein or lactose concentration, but oilseed-based supplements increased milk yield compared with the control diet and milk fat concentration relative to control was reduced by 4 g/kg by supplemental EL.
29914588	4	115	dep	cows	668:671	arg1	Holstein-Friesian					650:666	Holstein-Friesian	650:666	Holstein-Friesian	650:666	Four multiparous, Holstein-Friesian cows in mid-lactation were randomly allocated to one of four treatment diets in a 4×4 Latin square design with 28-day periods.
29914588	11	116	theme	oilseed-based	2262:2274	arg1	preparations					2276:2287	these oilseed-based preparations	2256:2287	these oilseed-based preparations	2256:2287	Supplementing dairy cow diets with these oilseed-based preparations affected milk FA profile and increased milk yield.
29914588	3	117	theme	fatty	607:611	arg1	FA					619:620	FA	619:620	FA	619:620	The objective was to investigate the effect of feeding different forms of supplemental plant oils on both methane emissions and milk fatty acid (FA) profile.
29914588	3	117	theme	fatty	607:611	arg1	acid					613:616	milk fatty acid	602:616	milk fatty acid (FA) profile	602:629	The objective was to investigate the effect of feeding different forms of supplemental plant oils on both methane emissions and milk fatty acid (FA) profile.
29914588	1	118	theme	rumen	267:271	arg1	fermentation					273:284	rumen fermentation	267:284	rumen fermentation	267:284	It is known that supplementing dairy cow diets with full-fat oilseeds can be used as a strategy to mitigate methane emissions, through their action on rumen fermentation.
29914588	10	119	theme	trans	2121:2125	arg1	FA					2127:2128	trans FA	2121:2128	cis-9 18:1 but also trans FA	2101:2128	All the fat supplements decreased milk total saturated fatty acid (SFA) concentration compared with the control, and SFA were replaced with mainly cis-9 18:1 but also trans FA (and in the case of EL and CPLO there were increases in polyunsaturated FA concentration).
29914588	4	120	theme	Latin	754:758	arg1	design					767:772	a 4×4 Latin square design	748:772	a 4×4 Latin square design with 28-day periods	748:792	Four multiparous, Holstein-Friesian cows in mid-lactation were randomly allocated to one of four treatment diets in a 4×4 Latin square design with 28-day periods.
29914588	12	121	theme	feeding	2440:2446	arg1	MR					2448:2449	feeding MR	2440:2449	feeding MR	2440:2449	However, only the linseed-based supplements reduced methane production, yield or intensity, whereas feeding MR had no effect.
29914588	11	122	theme	FA	2303:2304	arg1	profile					2306:2312	milk FA profile	2298:2312	milk FA profile	2298:2312	Supplementing dairy cow diets with these oilseed-based preparations affected milk FA profile and increased milk yield.
29914588	5	123	dep	fed	806:808	arg1	basis					893:897	dry matter (DM) basis	877:897	dry matter (DM) basis	877:897	Diets were fed as a total mixed ration with a 50 : 50 forage : concentrate ratio (dry matter (DM) basis) with the forage consisting of 75 : 25 maize silage : grass silage (DM).
29914588	5	123	dep	fed	806:808	arg1	ratio					870:874	concentrate ratio	858:874	concentrate ratio (dry matter (DM) basis) with the forage consisting of 75 : 25 maize silage : grass silage (DM)	858:969	Diets were fed as a total mixed ration with a 50 : 50 forage : concentrate ratio (dry matter (DM) basis) with the forage consisting of 75 : 25 maize silage : grass silage (DM).
29914588	5	124	theme	DM	889:890	arg1	basis					893:897	dry matter (DM) basis	877:897	dry matter (DM) basis	877:897	Diets were fed as a total mixed ration with a 50 : 50 forage : concentrate ratio (dry matter (DM) basis) with the forage consisting of 75 : 25 maize silage : grass silage (DM).
29914588	5	124	theme	DM	889:890	arg1	ratio					870:874	concentrate ratio	858:874	concentrate ratio (dry matter (DM) basis) with the forage consisting of 75 : 25 maize silage : grass silage (DM)	858:969	Diets were fed as a total mixed ration with a 50 : 50 forage : concentrate ratio (dry matter (DM) basis) with the forage consisting of 75 : 25 maize silage : grass silage (DM).
29914588	6	125	theme	Dietary	972:978	arg1	diet					1006:1009	a control diet	996:1009	a control diet containing no supplemental fat	996:1040	Dietary treatments were a control diet containing no supplemental fat, and three treatment diets containing extruded linseed (EL), calcium salts of palm and linseed oil (CPLO) or milled rapeseed (MR) formulated to provide each cow with an estimated 500 g additional oil/day (22 g oil/kg diet DM).
29914588	6	125	theme	Dietary	972:978	arg1	treatments					980:989	Dietary treatments	972:989	Dietary treatments	972:989	Dietary treatments were a control diet containing no supplemental fat, and three treatment diets containing extruded linseed (EL), calcium salts of palm and linseed oil (CPLO) or milled rapeseed (MR) formulated to provide each cow with an estimated 500 g additional oil/day (22 g oil/kg diet DM).
29914588	4	126	theme	diets	739:743	arg1	diets					739:743	four treatment diets	724:743	four treatment diets	724:743	Four multiparous, Holstein-Friesian cows in mid-lactation were randomly allocated to one of four treatment diets in a 4×4 Latin square design with 28-day periods.
29914588	4	126	theme	diets	739:743	arg1	one					717:719	one	717:719	one	717:719	Four multiparous, Holstein-Friesian cows in mid-lactation were randomly allocated to one of four treatment diets in a 4×4 Latin square design with 28-day periods.
29914588	2	127	theme	direct	296:301	arg1	comparisons					303:313	direct comparisons	296:313	direct comparisons of the effect of different oil sources	296:352	However, direct comparisons of the effect of different oil sources are very few, as are studies implementing supplementation levels that reflect what is commonly fed on commercial farms.
29914588	4	128	from	cows	668:671	arg1	mid-lactation					676:688	mid-lactation	676:688	mid-lactation	676:688	Four multiparous, Holstein-Friesian cows in mid-lactation were randomly allocated to one of four treatment diets in a 4×4 Latin square design with 28-day periods.
29914588	2	129	theme	different	332:340	arg1	sources					346:352	different oil sources	332:352	different oil sources	332:352	However, direct comparisons of the effect of different oil sources are very few, as are studies implementing supplementation levels that reflect what is commonly fed on commercial farms.
29914588	6	130	theme	diet	1259:1262	arg1	oil/day					1238:1244	an estimated 500 g additional oil/day	1208:1244	an estimated 500 g additional oil/day (22 g oil/kg diet DM)	1208:1266	Dietary treatments were a control diet containing no supplemental fat, and three treatment diets containing extruded linseed (EL), calcium salts of palm and linseed oil (CPLO) or milled rapeseed (MR) formulated to provide each cow with an estimated 500 g additional oil/day (22 g oil/kg diet DM).
29914588	6	130	theme	diet	1259:1262	arg1	DM					1264:1265	22 g oil/kg diet DM	1247:1265	22 g oil/kg diet DM	1247:1265	Dietary treatments were a control diet containing no supplemental fat, and three treatment diets containing extruded linseed (EL), calcium salts of palm and linseed oil (CPLO) or milled rapeseed (MR) formulated to provide each cow with an estimated 500 g additional oil/day (22 g oil/kg diet DM).
30956991	6	0	theme	NOD	1560:1562	arg1	mice					1564:1567	NOD mice	1560:1567	NOD mice	1560:1567	It is, therefore, concluded that early life oral E. coli LPS has an impact on the local immune response, which, however, did not influence T1D incidence in NOD mice later in life.
30956991	2	1	theme	Akkermansia	528:538	arg1	muciniphila					540:550	the mucin-metabolising bacterium Akkermansia muciniphila	495:550	the mucin-metabolising bacterium Akkermansia muciniphila	495:550	Previously, a reduced incidence of T1D accompanied by dramatically increased abundances of both the mucin-metabolising bacterium Akkermansia muciniphila, and LPS-carrying Proteobacteria was observed, when vancomycin was given to pups of nonobese diabetic (NOD) mice.
30956991	5	2	theme	genetic	1259:1265	arg1	markers					1267:1273	the genetic markers	1255:1273	the genetic markers of gut permeability, Claudin8, Occludin, Zonulin-1 (Tjp1), Claudin15, Muc1, and Muc2	1255:1358	However, no significant difference in the incidence of T1D, gut microbiota composition, or ileum expression of the genetic markers of gut permeability, Claudin8, Occludin, Zonulin-1 (Tjp1), Claudin15, Muc1, and Muc2 were observed in relation to LPS ingestion.
30956991	6	3	from	incidence	1547:1555	arg1	mice					1564:1567	NOD mice	1560:1567	NOD mice	1560:1567	It is, therefore, concluded that early life oral E. coli LPS has an impact on the local immune response, which, however, did not influence T1D incidence in NOD mice later in life.
30956991	0	4	theme	Oral	0:3	arg1	Dosing					9:14	Oral LPS Dosing	0:14	Oral LPS Dosing	0:14	Oral LPS Dosing Induces Local Immunological Changes in the Pancreatic Lymph Nodes in Mice.
30956991	1	5	theme	molecular	170:178	arg1	patterns					180:187	microbial-associated molecular patterns	149:187	microbial-associated molecular patterns (MAMPs)	149:195	Lacking the initial contact between the immune system and microbial-associated molecular patterns (MAMPs), such as lipopolysaccharides (LPS), early in life, may be regarded as one of the causal factors of the increasing global increase in the incidence of autoimmune diseases, such as type 1 diabetes (T1D).
30956991	1	5	theme	molecular	170:178	arg1	factors					285:291	the causal factors	274:291	the causal factors of the increasing global increase in the incidence of autoimmune diseases, such as type 1 diabetes (T1D)	274:396	Lacking the initial contact between the immune system and microbial-associated molecular patterns (MAMPs), such as lipopolysaccharides (LPS), early in life, may be regarded as one of the causal factors of the increasing global increase in the incidence of autoimmune diseases, such as type 1 diabetes (T1D).
30956991	1	5	theme	molecular	170:178	arg1	one					267:269	one	267:269	one	267:269	Lacking the initial contact between the immune system and microbial-associated molecular patterns (MAMPs), such as lipopolysaccharides (LPS), early in life, may be regarded as one of the causal factors of the increasing global increase in the incidence of autoimmune diseases, such as type 1 diabetes (T1D).
30956991	1	5	theme	molecular	170:178	arg1	lipopolysaccharides					206:224	lipopolysaccharides	206:224	lipopolysaccharides (LPS)	206:230	Lacking the initial contact between the immune system and microbial-associated molecular patterns (MAMPs), such as lipopolysaccharides (LPS), early in life, may be regarded as one of the causal factors of the increasing global increase in the incidence of autoimmune diseases, such as type 1 diabetes (T1D).
30956991	1	5	theme	molecular	170:178	arg1	MAMPs					190:194	MAMPs	190:194	MAMPs	190:194	Lacking the initial contact between the immune system and microbial-associated molecular patterns (MAMPs), such as lipopolysaccharides (LPS), early in life, may be regarded as one of the causal factors of the increasing global increase in the incidence of autoimmune diseases, such as type 1 diabetes (T1D).
30956991	2	6	theme	LPS-carrying	557:568	arg1	Proteobacteria					570:583	LPS-carrying Proteobacteria	557:583	LPS-carrying Proteobacteria	557:583	Previously, a reduced incidence of T1D accompanied by dramatically increased abundances of both the mucin-metabolising bacterium Akkermansia muciniphila, and LPS-carrying Proteobacteria was observed, when vancomycin was given to pups of nonobese diabetic (NOD) mice.
30956991	3	7	theme	T1D	676:678	arg1	effect					699:704	the T1D incidence reducing effect	672:704	the T1D incidence reducing effect of A. muciniphila	672:722	While the T1D incidence reducing effect of A. muciniphila has been shown in further studies, little is known as to whether the increased abundance of LPS-carrying bacteria also has a protective effect.
30956991	5	8	theme	ileum	1235:1239	arg1	incidence					1186:1194	the incidence	1182:1194	the incidence of T1D, gut microbiota composition, or ileum expression of the genetic markers of gut permeability, Claudin8, Occludin, Zonulin-1 (Tjp1), Claudin15, Muc1, and Muc2	1182:1358	However, no significant difference in the incidence of T1D, gut microbiota composition, or ileum expression of the genetic markers of gut permeability, Claudin8, Occludin, Zonulin-1 (Tjp1), Claudin15, Muc1, and Muc2 were observed in relation to LPS ingestion.
30956991	3	9	contain	has	843:845	arg2	effect					860:865	a protective effect	847:865	a protective effect	847:865	While the T1D incidence reducing effect of A. muciniphila has been shown in further studies, little is known as to whether the increased abundance of LPS-carrying bacteria also has a protective effect.
30956991	3	9	contain	has	843:845	arg1	abundance					803:811	the increased abundance	789:811	the increased abundance of LPS-carrying bacteria	789:836	While the T1D incidence reducing effect of A. muciniphila has been shown in further studies, little is known as to whether the increased abundance of LPS-carrying bacteria also has a protective effect.
30956991	4	10	theme	TNFα	990:993	arg1	expressions					975:985	the gene expressions	966:985	the gene expressions of TNFα, IL-10, IL-6, IFNγ, IL-1β, IL-2, IL-4, and FoxP3 in the pancreatic lymph nodes	966:1072	Therefore, we fed NOD pups with Eschericia coli LPS orally from birth to weaning, which decreased the gene expressions of TNFα, IL-10, IL-6, IFNγ, IL-1β, IL-2, IL-4, and FoxP3 in the pancreatic lymph nodes, while the same gene expression profile in the spleen was unaffected.
30956991	1	11	theme	initial	103:109	arg1	contact					111:117	the initial contact	99:117	the initial contact between the immune system	99:143	Lacking the initial contact between the immune system and microbial-associated molecular patterns (MAMPs), such as lipopolysaccharides (LPS), early in life, may be regarded as one of the causal factors of the increasing global increase in the incidence of autoimmune diseases, such as type 1 diabetes (T1D).
30956991	2	12	theme	mucin-metabolising	499:516	arg1	muciniphila					540:550	the mucin-metabolising bacterium Akkermansia muciniphila	495:550	the mucin-metabolising bacterium Akkermansia muciniphila	495:550	Previously, a reduced incidence of T1D accompanied by dramatically increased abundances of both the mucin-metabolising bacterium Akkermansia muciniphila, and LPS-carrying Proteobacteria was observed, when vancomycin was given to pups of nonobese diabetic (NOD) mice.
30956991	6	13	theme	oral	1448:1451	arg1	LPS					1461:1463	early life oral E. coli LPS	1437:1463	early life oral E. coli LPS	1437:1463	It is, therefore, concluded that early life oral E. coli LPS has an impact on the local immune response, which, however, did not influence T1D incidence in NOD mice later in life.
30956991	2	14	theme	diabetic	645:652	arg1	mice					660:663	nonobese diabetic (NOD) mice	636:663	nonobese diabetic (NOD) mice	636:663	Previously, a reduced incidence of T1D accompanied by dramatically increased abundances of both the mucin-metabolising bacterium Akkermansia muciniphila, and LPS-carrying Proteobacteria was observed, when vancomycin was given to pups of nonobese diabetic (NOD) mice.
30956991	3	15	theme	reducing	690:697	arg1	effect					699:704	the T1D incidence reducing effect	672:704	the T1D incidence reducing effect of A. muciniphila	672:722	While the T1D incidence reducing effect of A. muciniphila has been shown in further studies, little is known as to whether the increased abundance of LPS-carrying bacteria also has a protective effect.
30956991	1	16	theme	autoimmune	347:356	arg1	diseases					358:365	autoimmune diseases	347:365	autoimmune diseases	347:365	Lacking the initial contact between the immune system and microbial-associated molecular patterns (MAMPs), such as lipopolysaccharides (LPS), early in life, may be regarded as one of the causal factors of the increasing global increase in the incidence of autoimmune diseases, such as type 1 diabetes (T1D).
30956991	1	16	theme	autoimmune	347:356	arg1	diabetes					383:390	type 1 diabetes	376:390	type 1 diabetes (T1D)	376:396	Lacking the initial contact between the immune system and microbial-associated molecular patterns (MAMPs), such as lipopolysaccharides (LPS), early in life, may be regarded as one of the causal factors of the increasing global increase in the incidence of autoimmune diseases, such as type 1 diabetes (T1D).
30956991	4	17	from	expressions	975:985	arg1	nodes					1068:1072	the pancreatic lymph nodes	1047:1072	the pancreatic lymph nodes	1047:1072	Therefore, we fed NOD pups with Eschericia coli LPS orally from birth to weaning, which decreased the gene expressions of TNFα, IL-10, IL-6, IFNγ, IL-1β, IL-2, IL-4, and FoxP3 in the pancreatic lymph nodes, while the same gene expression profile in the spleen was unaffected.
30956991	6	18	theme	early	1437:1441	arg1	LPS					1461:1463	early life oral E. coli LPS	1437:1463	early life oral E. coli LPS	1437:1463	It is, therefore, concluded that early life oral E. coli LPS has an impact on the local immune response, which, however, did not influence T1D incidence in NOD mice later in life.
30956991	5	19	from	difference	1168:1177	arg1	incidence					1186:1194	the incidence	1182:1194	the incidence of T1D, gut microbiota composition, or ileum expression of the genetic markers of gut permeability, Claudin8, Occludin, Zonulin-1 (Tjp1), Claudin15, Muc1, and Muc2	1182:1358	However, no significant difference in the incidence of T1D, gut microbiota composition, or ileum expression of the genetic markers of gut permeability, Claudin8, Occludin, Zonulin-1 (Tjp1), Claudin15, Muc1, and Muc2 were observed in relation to LPS ingestion.
30956991	1	20	from	patterns	180:187	arg1	life					242:245	life	242:245	life	242:245	Lacking the initial contact between the immune system and microbial-associated molecular patterns (MAMPs), such as lipopolysaccharides (LPS), early in life, may be regarded as one of the causal factors of the increasing global increase in the incidence of autoimmune diseases, such as type 1 diabetes (T1D).
30956991	3	21	theme	protective	849:858	arg1	effect					860:865	a protective effect	847:865	a protective effect	847:865	While the T1D incidence reducing effect of A. muciniphila has been shown in further studies, little is known as to whether the increased abundance of LPS-carrying bacteria also has a protective effect.
30956991	2	22	theme	reduced	413:419	arg1	incidence					421:429	a reduced incidence	411:429	a reduced incidence of T1D accompanied by dramatically increased abundances of both the mucin-metabolising bacterium Akkermansia muciniphila, and LPS-carrying Proteobacteria	411:583	Previously, a reduced incidence of T1D accompanied by dramatically increased abundances of both the mucin-metabolising bacterium Akkermansia muciniphila, and LPS-carrying Proteobacteria was observed, when vancomycin was given to pups of nonobese diabetic (NOD) mice.
30956991	2	23	theme	mice	660:663	arg1	pups					628:631	pups	628:631	pups of nonobese diabetic (NOD) mice	628:663	Previously, a reduced incidence of T1D accompanied by dramatically increased abundances of both the mucin-metabolising bacterium Akkermansia muciniphila, and LPS-carrying Proteobacteria was observed, when vancomycin was given to pups of nonobese diabetic (NOD) mice.
30956991	3	24	theme	muciniphila	712:722	arg1	effect					699:704	the T1D incidence reducing effect	672:704	the T1D incidence reducing effect of A. muciniphila	672:722	While the T1D incidence reducing effect of A. muciniphila has been shown in further studies, little is known as to whether the increased abundance of LPS-carrying bacteria also has a protective effect.
30956991	1	25	theme	immune	131:136	arg1	system					138:143	the immune system	127:143	the immune system	127:143	Lacking the initial contact between the immune system and microbial-associated molecular patterns (MAMPs), such as lipopolysaccharides (LPS), early in life, may be regarded as one of the causal factors of the increasing global increase in the incidence of autoimmune diseases, such as type 1 diabetes (T1D).
30956991	6	26	theme	immune	1492:1497	arg1	response					1499:1506	the local immune response	1482:1506	the local immune response	1482:1506	It is, therefore, concluded that early life oral E. coli LPS has an impact on the local immune response, which, however, did not influence T1D incidence in NOD mice later in life.
30956991	5	27	theme	Claudin8	1296:1303	arg1	markers					1267:1273	the genetic markers	1255:1273	the genetic markers of gut permeability, Claudin8, Occludin, Zonulin-1 (Tjp1), Claudin15, Muc1, and Muc2	1255:1358	However, no significant difference in the incidence of T1D, gut microbiota composition, or ileum expression of the genetic markers of gut permeability, Claudin8, Occludin, Zonulin-1 (Tjp1), Claudin15, Muc1, and Muc2 were observed in relation to LPS ingestion.
30956991	2	28	theme	NOD	655:657	arg1	mice					660:663	nonobese diabetic (NOD) mice	636:663	nonobese diabetic (NOD) mice	636:663	Previously, a reduced incidence of T1D accompanied by dramatically increased abundances of both the mucin-metabolising bacterium Akkermansia muciniphila, and LPS-carrying Proteobacteria was observed, when vancomycin was given to pups of nonobese diabetic (NOD) mice.
30956991	0	29	theme	Lymph	70:74	arg1	Nodes					76:80	the Pancreatic Lymph Nodes	55:80	the Pancreatic Lymph Nodes in Mice	55:88	Oral LPS Dosing Induces Local Immunological Changes in the Pancreatic Lymph Nodes in Mice.
30956991	1	30	theme	factors	285:291	arg1	factors					285:291	the causal factors	274:291	the causal factors of the increasing global increase in the incidence of autoimmune diseases, such as type 1 diabetes (T1D)	274:396	Lacking the initial contact between the immune system and microbial-associated molecular patterns (MAMPs), such as lipopolysaccharides (LPS), early in life, may be regarded as one of the causal factors of the increasing global increase in the incidence of autoimmune diseases, such as type 1 diabetes (T1D).
30956991	1	30	theme	factors	285:291	arg1	patterns					180:187	microbial-associated molecular patterns	149:187	microbial-associated molecular patterns (MAMPs)	149:195	Lacking the initial contact between the immune system and microbial-associated molecular patterns (MAMPs), such as lipopolysaccharides (LPS), early in life, may be regarded as one of the causal factors of the increasing global increase in the incidence of autoimmune diseases, such as type 1 diabetes (T1D).
30956991	1	30	theme	factors	285:291	arg1	one					267:269	one	267:269	one	267:269	Lacking the initial contact between the immune system and microbial-associated molecular patterns (MAMPs), such as lipopolysaccharides (LPS), early in life, may be regarded as one of the causal factors of the increasing global increase in the incidence of autoimmune diseases, such as type 1 diabetes (T1D).
30956991	1	30	theme	factors	285:291	arg1	lipopolysaccharides					206:224	lipopolysaccharides	206:224	lipopolysaccharides (LPS)	206:230	Lacking the initial contact between the immune system and microbial-associated molecular patterns (MAMPs), such as lipopolysaccharides (LPS), early in life, may be regarded as one of the causal factors of the increasing global increase in the incidence of autoimmune diseases, such as type 1 diabetes (T1D).
30956991	5	31	theme	Occludin	1306:1313	arg1	markers					1267:1273	the genetic markers	1255:1273	the genetic markers of gut permeability, Claudin8, Occludin, Zonulin-1 (Tjp1), Claudin15, Muc1, and Muc2	1255:1358	However, no significant difference in the incidence of T1D, gut microbiota composition, or ileum expression of the genetic markers of gut permeability, Claudin8, Occludin, Zonulin-1 (Tjp1), Claudin15, Muc1, and Muc2 were observed in relation to LPS ingestion.
30956991	3	32	theme	LPS-carrying	816:827	arg1	bacteria					829:836	LPS-carrying bacteria	816:836	LPS-carrying bacteria	816:836	While the T1D incidence reducing effect of A. muciniphila has been shown in further studies, little is known as to whether the increased abundance of LPS-carrying bacteria also has a protective effect.
30956991	5	33	theme	significant	1156:1166	arg1	difference					1168:1177	no significant difference	1153:1177	no significant difference in the incidence of T1D, gut microbiota composition, or ileum expression of the genetic markers of gut permeability, Claudin8, Occludin, Zonulin-1 (Tjp1), Claudin15, Muc1, and Muc2	1153:1358	However, no significant difference in the incidence of T1D, gut microbiota composition, or ileum expression of the genetic markers of gut permeability, Claudin8, Occludin, Zonulin-1 (Tjp1), Claudin15, Muc1, and Muc2 were observed in relation to LPS ingestion.
30956991	1	34	theme	global	311:316	arg1	increase					318:325	the increasing global increase	296:325	the increasing global increase in the incidence of autoimmune diseases, such as type 1 diabetes (T1D)	296:396	Lacking the initial contact between the immune system and microbial-associated molecular patterns (MAMPs), such as lipopolysaccharides (LPS), early in life, may be regarded as one of the causal factors of the increasing global increase in the incidence of autoimmune diseases, such as type 1 diabetes (T1D).
30956991	0	35	theme	Immunological	30:42	arg1	Changes					44:50	Local Immunological Changes	24:50	Local Immunological Changes	24:50	Oral LPS Dosing Induces Local Immunological Changes in the Pancreatic Lymph Nodes in Mice.
30956991	5	36	theme	permeability	1282:1293	arg1	markers					1267:1273	the genetic markers	1255:1273	the genetic markers of gut permeability, Claudin8, Occludin, Zonulin-1 (Tjp1), Claudin15, Muc1, and Muc2	1255:1358	However, no significant difference in the incidence of T1D, gut microbiota composition, or ileum expression of the genetic markers of gut permeability, Claudin8, Occludin, Zonulin-1 (Tjp1), Claudin15, Muc1, and Muc2 were observed in relation to LPS ingestion.
30956991	1	37	from	increase	318:325	arg1	incidence					334:342	the incidence	330:342	the incidence of autoimmune diseases, such as type 1 diabetes (T1D)	330:396	Lacking the initial contact between the immune system and microbial-associated molecular patterns (MAMPs), such as lipopolysaccharides (LPS), early in life, may be regarded as one of the causal factors of the increasing global increase in the incidence of autoimmune diseases, such as type 1 diabetes (T1D).
30956991	4	38	theme	pancreatic	1051:1060	arg1	nodes					1068:1072	the pancreatic lymph nodes	1047:1072	the pancreatic lymph nodes	1047:1072	Therefore, we fed NOD pups with Eschericia coli LPS orally from birth to weaning, which decreased the gene expressions of TNFα, IL-10, IL-6, IFNγ, IL-1β, IL-2, IL-4, and FoxP3 in the pancreatic lymph nodes, while the same gene expression profile in the spleen was unaffected.
30956991	4	39	theme	Eschericia	900:909	arg1	LPS					916:918	Eschericia coli LPS	900:918	Eschericia coli LPS	900:918	Therefore, we fed NOD pups with Eschericia coli LPS orally from birth to weaning, which decreased the gene expressions of TNFα, IL-10, IL-6, IFNγ, IL-1β, IL-2, IL-4, and FoxP3 in the pancreatic lymph nodes, while the same gene expression profile in the spleen was unaffected.
30956991	0	40	theme	Local	24:28	arg1	Changes					44:50	Local Immunological Changes	24:50	Local Immunological Changes	24:50	Oral LPS Dosing Induces Local Immunological Changes in the Pancreatic Lymph Nodes in Mice.
30956991	4	41	with	pups	890:893	arg1	LPS					916:918	Eschericia coli LPS	900:918	Eschericia coli LPS	900:918	Therefore, we fed NOD pups with Eschericia coli LPS orally from birth to weaning, which decreased the gene expressions of TNFα, IL-10, IL-6, IFNγ, IL-1β, IL-2, IL-4, and FoxP3 in the pancreatic lymph nodes, while the same gene expression profile in the spleen was unaffected.
30956991	4	42	from	profile	1106:1112	arg1	spleen					1121:1126	the spleen	1117:1126	the spleen	1117:1126	Therefore, we fed NOD pups with Eschericia coli LPS orally from birth to weaning, which decreased the gene expressions of TNFα, IL-10, IL-6, IFNγ, IL-1β, IL-2, IL-4, and FoxP3 in the pancreatic lymph nodes, while the same gene expression profile in the spleen was unaffected.
30956991	5	43	located	observed	1365:1372	arg2	difference					1168:1177	no significant difference	1153:1177	no significant difference in the incidence of T1D, gut microbiota composition, or ileum expression of the genetic markers of gut permeability, Claudin8, Occludin, Zonulin-1 (Tjp1), Claudin15, Muc1, and Muc2	1153:1358	However, no significant difference in the incidence of T1D, gut microbiota composition, or ileum expression of the genetic markers of gut permeability, Claudin8, Occludin, Zonulin-1 (Tjp1), Claudin15, Muc1, and Muc2 were observed in relation to LPS ingestion.
30956991	5	43	located	observed	1365:1372	arg1	relation					1377:1384	relation	1377:1384	relation to LPS ingestion	1377:1401	However, no significant difference in the incidence of T1D, gut microbiota composition, or ileum expression of the genetic markers of gut permeability, Claudin8, Occludin, Zonulin-1 (Tjp1), Claudin15, Muc1, and Muc2 were observed in relation to LPS ingestion.
30956991	4	44	theme	gene	970:973	arg1	expressions					975:985	the gene expressions	966:985	the gene expressions of TNFα, IL-10, IL-6, IFNγ, IL-1β, IL-2, IL-4, and FoxP3 in the pancreatic lymph nodes	966:1072	Therefore, we fed NOD pups with Eschericia coli LPS orally from birth to weaning, which decreased the gene expressions of TNFα, IL-10, IL-6, IFNγ, IL-1β, IL-2, IL-4, and FoxP3 in the pancreatic lymph nodes, while the same gene expression profile in the spleen was unaffected.
30956991	4	45	theme	same	1085:1088	arg1	unaffected					1132:1141	unaffected	1132:1141	unaffected	1132:1141	Therefore, we fed NOD pups with Eschericia coli LPS orally from birth to weaning, which decreased the gene expressions of TNFα, IL-10, IL-6, IFNγ, IL-1β, IL-2, IL-4, and FoxP3 in the pancreatic lymph nodes, while the same gene expression profile in the spleen was unaffected.
30956991	4	45	theme	same	1085:1088	arg1	profile					1106:1112	the same gene expression profile	1081:1112	the same gene expression profile in the spleen	1081:1126	Therefore, we fed NOD pups with Eschericia coli LPS orally from birth to weaning, which decreased the gene expressions of TNFα, IL-10, IL-6, IFNγ, IL-1β, IL-2, IL-4, and FoxP3 in the pancreatic lymph nodes, while the same gene expression profile in the spleen was unaffected.
30956991	5	46	theme	composition	1219:1229	arg1	incidence					1186:1194	the incidence	1182:1194	the incidence of T1D, gut microbiota composition, or ileum expression of the genetic markers of gut permeability, Claudin8, Occludin, Zonulin-1 (Tjp1), Claudin15, Muc1, and Muc2	1182:1358	However, no significant difference in the incidence of T1D, gut microbiota composition, or ileum expression of the genetic markers of gut permeability, Claudin8, Occludin, Zonulin-1 (Tjp1), Claudin15, Muc1, and Muc2 were observed in relation to LPS ingestion.
30956991	4	47	theme	expression	1095:1104	arg1	unaffected					1132:1141	unaffected	1132:1141	unaffected	1132:1141	Therefore, we fed NOD pups with Eschericia coli LPS orally from birth to weaning, which decreased the gene expressions of TNFα, IL-10, IL-6, IFNγ, IL-1β, IL-2, IL-4, and FoxP3 in the pancreatic lymph nodes, while the same gene expression profile in the spleen was unaffected.
30956991	4	47	theme	expression	1095:1104	arg1	profile					1106:1112	the same gene expression profile	1081:1112	the same gene expression profile in the spleen	1081:1126	Therefore, we fed NOD pups with Eschericia coli LPS orally from birth to weaning, which decreased the gene expressions of TNFα, IL-10, IL-6, IFNγ, IL-1β, IL-2, IL-4, and FoxP3 in the pancreatic lymph nodes, while the same gene expression profile in the spleen was unaffected.
30956991	5	48	theme	T1D	1199:1201	arg1	incidence					1186:1194	the incidence	1182:1194	the incidence of T1D, gut microbiota composition, or ileum expression of the genetic markers of gut permeability, Claudin8, Occludin, Zonulin-1 (Tjp1), Claudin15, Muc1, and Muc2	1182:1358	However, no significant difference in the incidence of T1D, gut microbiota composition, or ileum expression of the genetic markers of gut permeability, Claudin8, Occludin, Zonulin-1 (Tjp1), Claudin15, Muc1, and Muc2 were observed in relation to LPS ingestion.
30956991	5	49	theme	Zonulin-1	1316:1324	arg1	markers					1267:1273	the genetic markers	1255:1273	the genetic markers of gut permeability, Claudin8, Occludin, Zonulin-1 (Tjp1), Claudin15, Muc1, and Muc2	1255:1358	However, no significant difference in the incidence of T1D, gut microbiota composition, or ileum expression of the genetic markers of gut permeability, Claudin8, Occludin, Zonulin-1 (Tjp1), Claudin15, Muc1, and Muc2 were observed in relation to LPS ingestion.
30956991	2	50	theme	muciniphila	540:550	arg1	abundances					476:485	dramatically increased abundances	453:485	dramatically increased abundances of both the mucin-metabolising bacterium Akkermansia muciniphila, and LPS-carrying Proteobacteria	453:583	Previously, a reduced incidence of T1D accompanied by dramatically increased abundances of both the mucin-metabolising bacterium Akkermansia muciniphila, and LPS-carrying Proteobacteria was observed, when vancomycin was given to pups of nonobese diabetic (NOD) mice.
30956991	4	51	dep	Eschericia	900:909	arg1	coli					911:914	coli	911:914	coli	911:914	Therefore, we fed NOD pups with Eschericia coli LPS orally from birth to weaning, which decreased the gene expressions of TNFα, IL-10, IL-6, IFNγ, IL-1β, IL-2, IL-4, and FoxP3 in the pancreatic lymph nodes, while the same gene expression profile in the spleen was unaffected.
30956991	1	52	theme	type	376:379	arg1	T1D					393:395	T1D	393:395	T1D	393:395	Lacking the initial contact between the immune system and microbial-associated molecular patterns (MAMPs), such as lipopolysaccharides (LPS), early in life, may be regarded as one of the causal factors of the increasing global increase in the incidence of autoimmune diseases, such as type 1 diabetes (T1D).
30956991	1	52	theme	type	376:379	arg1	diabetes					383:390	type 1 diabetes	376:390	type 1 diabetes (T1D)	376:396	Lacking the initial contact between the immune system and microbial-associated molecular patterns (MAMPs), such as lipopolysaccharides (LPS), early in life, may be regarded as one of the causal factors of the increasing global increase in the incidence of autoimmune diseases, such as type 1 diabetes (T1D).
30956991	2	53	theme	bacterium	518:526	arg1	muciniphila					540:550	the mucin-metabolising bacterium Akkermansia muciniphila	495:550	the mucin-metabolising bacterium Akkermansia muciniphila	495:550	Previously, a reduced incidence of T1D accompanied by dramatically increased abundances of both the mucin-metabolising bacterium Akkermansia muciniphila, and LPS-carrying Proteobacteria was observed, when vancomycin was given to pups of nonobese diabetic (NOD) mice.
30956991	6	54	theme	T1D	1543:1545	arg1	incidence					1547:1555	T1D incidence	1543:1555	T1D incidence in NOD mice	1543:1567	It is, therefore, concluded that early life oral E. coli LPS has an impact on the local immune response, which, however, did not influence T1D incidence in NOD mice later in life.
30956991	5	55	theme	Muc2	1355:1358	arg1	markers					1267:1273	the genetic markers	1255:1273	the genetic markers of gut permeability, Claudin8, Occludin, Zonulin-1 (Tjp1), Claudin15, Muc1, and Muc2	1255:1358	However, no significant difference in the incidence of T1D, gut microbiota composition, or ileum expression of the genetic markers of gut permeability, Claudin8, Occludin, Zonulin-1 (Tjp1), Claudin15, Muc1, and Muc2 were observed in relation to LPS ingestion.
30956991	5	56	theme	markers	1267:1273	arg1	ileum					1235:1239	ileum	1235:1239	ileum	1235:1239	However, no significant difference in the incidence of T1D, gut microbiota composition, or ileum expression of the genetic markers of gut permeability, Claudin8, Occludin, Zonulin-1 (Tjp1), Claudin15, Muc1, and Muc2 were observed in relation to LPS ingestion.
30956991	5	56	theme	markers	1267:1273	arg1	T1D					1199:1201	T1D	1199:1201	T1D	1199:1201	However, no significant difference in the incidence of T1D, gut microbiota composition, or ileum expression of the genetic markers of gut permeability, Claudin8, Occludin, Zonulin-1 (Tjp1), Claudin15, Muc1, and Muc2 were observed in relation to LPS ingestion.
30956991	5	56	theme	markers	1267:1273	arg1	composition					1219:1229	gut microbiota composition	1204:1229	gut microbiota composition	1204:1229	However, no significant difference in the incidence of T1D, gut microbiota composition, or ileum expression of the genetic markers of gut permeability, Claudin8, Occludin, Zonulin-1 (Tjp1), Claudin15, Muc1, and Muc2 were observed in relation to LPS ingestion.
30956991	1	57	theme	microbial-associated	149:168	arg1	patterns					180:187	microbial-associated molecular patterns	149:187	microbial-associated molecular patterns (MAMPs)	149:195	Lacking the initial contact between the immune system and microbial-associated molecular patterns (MAMPs), such as lipopolysaccharides (LPS), early in life, may be regarded as one of the causal factors of the increasing global increase in the incidence of autoimmune diseases, such as type 1 diabetes (T1D).
30956991	1	57	theme	microbial-associated	149:168	arg1	factors					285:291	the causal factors	274:291	the causal factors of the increasing global increase in the incidence of autoimmune diseases, such as type 1 diabetes (T1D)	274:396	Lacking the initial contact between the immune system and microbial-associated molecular patterns (MAMPs), such as lipopolysaccharides (LPS), early in life, may be regarded as one of the causal factors of the increasing global increase in the incidence of autoimmune diseases, such as type 1 diabetes (T1D).
30956991	1	57	theme	microbial-associated	149:168	arg1	one					267:269	one	267:269	one	267:269	Lacking the initial contact between the immune system and microbial-associated molecular patterns (MAMPs), such as lipopolysaccharides (LPS), early in life, may be regarded as one of the causal factors of the increasing global increase in the incidence of autoimmune diseases, such as type 1 diabetes (T1D).
30956991	1	57	theme	microbial-associated	149:168	arg1	lipopolysaccharides					206:224	lipopolysaccharides	206:224	lipopolysaccharides (LPS)	206:230	Lacking the initial contact between the immune system and microbial-associated molecular patterns (MAMPs), such as lipopolysaccharides (LPS), early in life, may be regarded as one of the causal factors of the increasing global increase in the incidence of autoimmune diseases, such as type 1 diabetes (T1D).
30956991	1	57	theme	microbial-associated	149:168	arg1	MAMPs					190:194	MAMPs	190:194	MAMPs	190:194	Lacking the initial contact between the immune system and microbial-associated molecular patterns (MAMPs), such as lipopolysaccharides (LPS), early in life, may be regarded as one of the causal factors of the increasing global increase in the incidence of autoimmune diseases, such as type 1 diabetes (T1D).
30956991	2	58	theme	Proteobacteria	570:583	arg1	abundances					476:485	dramatically increased abundances	453:485	dramatically increased abundances of both the mucin-metabolising bacterium Akkermansia muciniphila, and LPS-carrying Proteobacteria	453:583	Previously, a reduced incidence of T1D accompanied by dramatically increased abundances of both the mucin-metabolising bacterium Akkermansia muciniphila, and LPS-carrying Proteobacteria was observed, when vancomycin was given to pups of nonobese diabetic (NOD) mice.
30956991	3	59	theme	incidence	680:688	arg1	effect					699:704	the T1D incidence reducing effect	672:704	the T1D incidence reducing effect of A. muciniphila	672:722	While the T1D incidence reducing effect of A. muciniphila has been shown in further studies, little is known as to whether the increased abundance of LPS-carrying bacteria also has a protective effect.
30956991	2	60	theme	increased	466:474	arg1	abundances					476:485	dramatically increased abundances	453:485	dramatically increased abundances of both the mucin-metabolising bacterium Akkermansia muciniphila, and LPS-carrying Proteobacteria	453:583	Previously, a reduced incidence of T1D accompanied by dramatically increased abundances of both the mucin-metabolising bacterium Akkermansia muciniphila, and LPS-carrying Proteobacteria was observed, when vancomycin was given to pups of nonobese diabetic (NOD) mice.
30956991	0	61	from	Nodes	76:80	arg1	Mice					85:88	Mice	85:88	Mice	85:88	Oral LPS Dosing Induces Local Immunological Changes in the Pancreatic Lymph Nodes in Mice.
30956991	5	62	theme	LPS	1389:1391	arg1	ingestion					1393:1401	LPS ingestion	1389:1401	LPS ingestion	1389:1401	However, no significant difference in the incidence of T1D, gut microbiota composition, or ileum expression of the genetic markers of gut permeability, Claudin8, Occludin, Zonulin-1 (Tjp1), Claudin15, Muc1, and Muc2 were observed in relation to LPS ingestion.
30956991	5	63	theme	gut	1204:1206	arg1	composition					1219:1229	gut microbiota composition	1204:1229	gut microbiota composition	1204:1229	However, no significant difference in the incidence of T1D, gut microbiota composition, or ileum expression of the genetic markers of gut permeability, Claudin8, Occludin, Zonulin-1 (Tjp1), Claudin15, Muc1, and Muc2 were observed in relation to LPS ingestion.
30956991	6	64	dep	E.	1453:1454	arg1	coli					1456:1459	coli	1456:1459	coli	1456:1459	It is, therefore, concluded that early life oral E. coli LPS has an impact on the local immune response, which, however, did not influence T1D incidence in NOD mice later in life.
30956991	6	65	theme	E.	1453:1454	arg1	LPS					1461:1463	early life oral E. coli LPS	1437:1463	early life oral E. coli LPS	1437:1463	It is, therefore, concluded that early life oral E. coli LPS has an impact on the local immune response, which, however, did not influence T1D incidence in NOD mice later in life.
30956991	6	66	theme	life	1443:1446	arg1	LPS					1461:1463	early life oral E. coli LPS	1437:1463	early life oral E. coli LPS	1437:1463	It is, therefore, concluded that early life oral E. coli LPS has an impact on the local immune response, which, however, did not influence T1D incidence in NOD mice later in life.
30956991	2	67	theme	nonobese	636:643	arg1	mice					660:663	nonobese diabetic (NOD) mice	636:663	nonobese diabetic (NOD) mice	636:663	Previously, a reduced incidence of T1D accompanied by dramatically increased abundances of both the mucin-metabolising bacterium Akkermansia muciniphila, and LPS-carrying Proteobacteria was observed, when vancomycin was given to pups of nonobese diabetic (NOD) mice.
30956991	4	68	dep	fed	882:884	arg1	decreased					956:964	decreased	956:964	decreased	956:964	Therefore, we fed NOD pups with Eschericia coli LPS orally from birth to weaning, which decreased the gene expressions of TNFα, IL-10, IL-6, IFNγ, IL-1β, IL-2, IL-4, and FoxP3 in the pancreatic lymph nodes, while the same gene expression profile in the spleen was unaffected.
30956991	1	69	theme	diseases	358:365	arg1	incidence					334:342	the incidence	330:342	the incidence of autoimmune diseases, such as type 1 diabetes (T1D)	330:396	Lacking the initial contact between the immune system and microbial-associated molecular patterns (MAMPs), such as lipopolysaccharides (LPS), early in life, may be regarded as one of the causal factors of the increasing global increase in the incidence of autoimmune diseases, such as type 1 diabetes (T1D).
30956991	0	70	theme	Pancreatic	59:68	arg1	Nodes					76:80	the Pancreatic Lymph Nodes	55:80	the Pancreatic Lymph Nodes in Mice	55:88	Oral LPS Dosing Induces Local Immunological Changes in the Pancreatic Lymph Nodes in Mice.
30956991	1	71	theme	causal	278:283	arg1	factors					285:291	the causal factors	274:291	the causal factors of the increasing global increase in the incidence of autoimmune diseases, such as type 1 diabetes (T1D)	274:396	Lacking the initial contact between the immune system and microbial-associated molecular patterns (MAMPs), such as lipopolysaccharides (LPS), early in life, may be regarded as one of the causal factors of the increasing global increase in the incidence of autoimmune diseases, such as type 1 diabetes (T1D).
30956991	3	72	theme	increased	793:801	arg1	abundance					803:811	the increased abundance	789:811	the increased abundance of LPS-carrying bacteria	789:836	While the T1D incidence reducing effect of A. muciniphila has been shown in further studies, little is known as to whether the increased abundance of LPS-carrying bacteria also has a protective effect.
30956991	4	73	theme	NOD	886:888	arg1	pups					890:893	NOD pups	886:893	NOD pups with Eschericia coli LPS	886:918	Therefore, we fed NOD pups with Eschericia coli LPS orally from birth to weaning, which decreased the gene expressions of TNFα, IL-10, IL-6, IFNγ, IL-1β, IL-2, IL-4, and FoxP3 in the pancreatic lymph nodes, while the same gene expression profile in the spleen was unaffected.
30956991	6	74	theme	local	1486:1490	arg1	response					1499:1506	the local immune response	1482:1506	the local immune response	1482:1506	It is, therefore, concluded that early life oral E. coli LPS has an impact on the local immune response, which, however, did not influence T1D incidence in NOD mice later in life.
30956991	1	75	from	incidence	334:342	arg1	factors					285:291	the causal factors	274:291	the causal factors of the increasing global increase in the incidence of autoimmune diseases, such as type 1 diabetes (T1D)	274:396	Lacking the initial contact between the immune system and microbial-associated molecular patterns (MAMPs), such as lipopolysaccharides (LPS), early in life, may be regarded as one of the causal factors of the increasing global increase in the incidence of autoimmune diseases, such as type 1 diabetes (T1D).
30956991	2	76	theme	T1D	434:436	arg1	incidence					421:429	a reduced incidence	411:429	a reduced incidence of T1D accompanied by dramatically increased abundances of both the mucin-metabolising bacterium Akkermansia muciniphila, and LPS-carrying Proteobacteria	411:583	Previously, a reduced incidence of T1D accompanied by dramatically increased abundances of both the mucin-metabolising bacterium Akkermansia muciniphila, and LPS-carrying Proteobacteria was observed, when vancomycin was given to pups of nonobese diabetic (NOD) mice.
30956991	3	77	theme	further	742:748	arg1	studies					750:756	further studies	742:756	further studies	742:756	While the T1D incidence reducing effect of A. muciniphila has been shown in further studies, little is known as to whether the increased abundance of LPS-carrying bacteria also has a protective effect.
30956991	1	78	theme	increasing	300:309	arg1	increase					318:325	the increasing global increase	296:325	the increasing global increase in the incidence of autoimmune diseases, such as type 1 diabetes (T1D)	296:396	Lacking the initial contact between the immune system and microbial-associated molecular patterns (MAMPs), such as lipopolysaccharides (LPS), early in life, may be regarded as one of the causal factors of the increasing global increase in the incidence of autoimmune diseases, such as type 1 diabetes (T1D).
30956991	3	79	theme	bacteria	829:836	arg1	abundance					803:811	the increased abundance	789:811	the increased abundance of LPS-carrying bacteria	789:836	While the T1D incidence reducing effect of A. muciniphila has been shown in further studies, little is known as to whether the increased abundance of LPS-carrying bacteria also has a protective effect.
30956991	6	80	from	impact	1472:1477	arg1	response					1499:1506	the local immune response	1482:1506	the local immune response	1482:1506	It is, therefore, concluded that early life oral E. coli LPS has an impact on the local immune response, which, however, did not influence T1D incidence in NOD mice later in life.
30956991	5	81	theme	gut	1278:1280	arg1	permeability					1282:1293	gut permeability	1278:1293	gut permeability	1278:1293	However, no significant difference in the incidence of T1D, gut microbiota composition, or ileum expression of the genetic markers of gut permeability, Claudin8, Occludin, Zonulin-1 (Tjp1), Claudin15, Muc1, and Muc2 were observed in relation to LPS ingestion.
30956991	0	82	theme	LPS	5:7	arg1	Dosing					9:14	Oral LPS Dosing	0:14	Oral LPS Dosing	0:14	Oral LPS Dosing Induces Local Immunological Changes in the Pancreatic Lymph Nodes in Mice.
30956991	1	83	theme	increase	318:325	arg1	factors					285:291	the causal factors	274:291	the causal factors of the increasing global increase in the incidence of autoimmune diseases, such as type 1 diabetes (T1D)	274:396	Lacking the initial contact between the immune system and microbial-associated molecular patterns (MAMPs), such as lipopolysaccharides (LPS), early in life, may be regarded as one of the causal factors of the increasing global increase in the incidence of autoimmune diseases, such as type 1 diabetes (T1D).
30956991	4	84	theme	lymph	1062:1066	arg1	nodes					1068:1072	the pancreatic lymph nodes	1047:1072	the pancreatic lymph nodes	1047:1072	Therefore, we fed NOD pups with Eschericia coli LPS orally from birth to weaning, which decreased the gene expressions of TNFα, IL-10, IL-6, IFNγ, IL-1β, IL-2, IL-4, and FoxP3 in the pancreatic lymph nodes, while the same gene expression profile in the spleen was unaffected.
30956991	5	85	dep	T1D	1199:1201	arg1	expression					1241:1250	expression	1241:1250	expression	1241:1250	However, no significant difference in the incidence of T1D, gut microbiota composition, or ileum expression of the genetic markers of gut permeability, Claudin8, Occludin, Zonulin-1 (Tjp1), Claudin15, Muc1, and Muc2 were observed in relation to LPS ingestion.
30956991	6	86	contain	has	1465:1467	arg2	impact					1472:1477	an impact	1469:1477	an impact on the local immune response, which, however, did not influence T1D incidence in NOD mice later in life	1469:1581	It is, therefore, concluded that early life oral E. coli LPS has an impact on the local immune response, which, however, did not influence T1D incidence in NOD mice later in life.
30956991	6	86	contain	has	1465:1467	arg1	LPS					1461:1463	early life oral E. coli LPS	1437:1463	early life oral E. coli LPS	1437:1463	It is, therefore, concluded that early life oral E. coli LPS has an impact on the local immune response, which, however, did not influence T1D incidence in NOD mice later in life.
30956991	5	87	theme	Claudin15	1334:1342	arg1	markers					1267:1273	the genetic markers	1255:1273	the genetic markers of gut permeability, Claudin8, Occludin, Zonulin-1 (Tjp1), Claudin15, Muc1, and Muc2	1255:1358	However, no significant difference in the incidence of T1D, gut microbiota composition, or ileum expression of the genetic markers of gut permeability, Claudin8, Occludin, Zonulin-1 (Tjp1), Claudin15, Muc1, and Muc2 were observed in relation to LPS ingestion.
30956991	1	88	from	factors	285:291	arg1	incidence					334:342	the incidence	330:342	the incidence of autoimmune diseases, such as type 1 diabetes (T1D)	330:396	Lacking the initial contact between the immune system and microbial-associated molecular patterns (MAMPs), such as lipopolysaccharides (LPS), early in life, may be regarded as one of the causal factors of the increasing global increase in the incidence of autoimmune diseases, such as type 1 diabetes (T1D).
30956991	5	89	theme	Muc1	1345:1348	arg1	markers					1267:1273	the genetic markers	1255:1273	the genetic markers of gut permeability, Claudin8, Occludin, Zonulin-1 (Tjp1), Claudin15, Muc1, and Muc2	1255:1358	However, no significant difference in the incidence of T1D, gut microbiota composition, or ileum expression of the genetic markers of gut permeability, Claudin8, Occludin, Zonulin-1 (Tjp1), Claudin15, Muc1, and Muc2 were observed in relation to LPS ingestion.
30956991	4	90	theme	gene	1090:1093	arg1	unaffected					1132:1141	unaffected	1132:1141	unaffected	1132:1141	Therefore, we fed NOD pups with Eschericia coli LPS orally from birth to weaning, which decreased the gene expressions of TNFα, IL-10, IL-6, IFNγ, IL-1β, IL-2, IL-4, and FoxP3 in the pancreatic lymph nodes, while the same gene expression profile in the spleen was unaffected.
30956991	4	90	theme	gene	1090:1093	arg1	profile					1106:1112	the same gene expression profile	1081:1112	the same gene expression profile in the spleen	1081:1126	Therefore, we fed NOD pups with Eschericia coli LPS orally from birth to weaning, which decreased the gene expressions of TNFα, IL-10, IL-6, IFNγ, IL-1β, IL-2, IL-4, and FoxP3 in the pancreatic lymph nodes, while the same gene expression profile in the spleen was unaffected.
30956991	5	91	theme	microbiota	1208:1217	arg1	composition					1219:1229	gut microbiota composition	1204:1229	gut microbiota composition	1204:1229	However, no significant difference in the incidence of T1D, gut microbiota composition, or ileum expression of the genetic markers of gut permeability, Claudin8, Occludin, Zonulin-1 (Tjp1), Claudin15, Muc1, and Muc2 were observed in relation to LPS ingestion.
30308299	7	0	theme	translational	1218:1230	arg1	therapy					1239:1245	translational cancer therapy	1218:1245	translational cancer therapy	1218:1245	This conveniently synthesized nanoparticle processes superior targeting capacity and biosecurity, supplying an effective approach to translational cancer therapy in the future.
30308299	3	1	theme	%	691:691	arg1	%					695:695	20%-30%	689:695	20%-30%	689:695	GA-ALG@Pt NPs were found equipped with intelligently ligand self-shielded property in which the internal GA could be induced to expose by initial cellular recognition, resulting in strengthened cellular uptake (20%-30%) and prolonged blood circulation time (3.43 times).
30308299	3	1	theme	%	691:691	arg1	uptake					681:686	strengthened cellular uptake	659:686	strengthened cellular uptake (20%-30%)	659:696	GA-ALG@Pt NPs were found equipped with intelligently ligand self-shielded property in which the internal GA could be induced to expose by initial cellular recognition, resulting in strengthened cellular uptake (20%-30%) and prolonged blood circulation time (3.43 times).
30308299	7	2	theme	synthesized	1103:1113	arg1	nanoparticle					1115:1126	This conveniently synthesized nanoparticle	1085:1126	This conveniently synthesized nanoparticle	1085:1126	This conveniently synthesized nanoparticle processes superior targeting capacity and biosecurity, supplying an effective approach to translational cancer therapy in the future.
30308299	1	3	theme	clinical	153:160	arg1	translation					162:172	The clinical translation	149:172	The clinical translation	149:172	The clinical translation remains a major challenge for platinum drug loaded nanoparticle due to the complexity of composition and preparation.
30308299	7	4	theme	cancer	1232:1237	arg1	therapy					1239:1245	translational cancer therapy	1218:1245	translational cancer therapy	1218:1245	This conveniently synthesized nanoparticle processes superior targeting capacity and biosecurity, supplying an effective approach to translational cancer therapy in the future.
30308299	6	5	theme	cisplatin	1074:1082	arg1	nephrotoxicity					1032:1045	nephrotoxicity	1032:1045	nephrotoxicity	1032:1045	Bio-safety is also a distinguishing feature of GA-ALG@Pt NPs that greatly relief the nephrotoxicity and systematic toxicity of cisplatin.
30308299	6	5	theme	cisplatin	1074:1082	arg1	toxicity					1062:1069	systematic toxicity	1051:1069	systematic toxicity	1051:1069	Bio-safety is also a distinguishing feature of GA-ALG@Pt NPs that greatly relief the nephrotoxicity and systematic toxicity of cisplatin.
30308299	3	6	theme	cellular	624:631	arg1	recognition					633:643	initial cellular recognition	616:643	initial cellular recognition	616:643	GA-ALG@Pt NPs were found equipped with intelligently ligand self-shielded property in which the internal GA could be induced to expose by initial cellular recognition, resulting in strengthened cellular uptake (20%-30%) and prolonged blood circulation time (3.43 times).
30308299	6	7	theme	distinguishing	968:981	arg1	feature					983:989	a distinguishing feature	966:989	a distinguishing feature of GA-ALG@Pt NPs that greatly relief the nephrotoxicity and systematic toxicity of cisplatin	966:1082	Bio-safety is also a distinguishing feature of GA-ALG@Pt NPs that greatly relief the nephrotoxicity and systematic toxicity of cisplatin.
30308299	6	7	theme	distinguishing	968:981	arg1	Bio-safety					947:956	Bio-safety	947:956	Bio-safety	947:956	Bio-safety is also a distinguishing feature of GA-ALG@Pt NPs that greatly relief the nephrotoxicity and systematic toxicity of cisplatin.
30308299	3	8	theme	ligand	531:536	arg1	property					552:559	intelligently ligand self-shielded property	517:559	intelligently ligand self-shielded property in which the internal GA could be induced to expose by initial cellular recognition, resulting in strengthened cellular uptake (20%-30%) and prolonged blood circulation time (3.43 times)	517:746	GA-ALG@Pt NPs were found equipped with intelligently ligand self-shielded property in which the internal GA could be induced to expose by initial cellular recognition, resulting in strengthened cellular uptake (20%-30%) and prolonged blood circulation time (3.43 times).
30308299	1	9	theme	composition	263:273	arg1	complexity					249:258	the complexity	245:258	the complexity of composition and preparation	245:289	The clinical translation remains a major challenge for platinum drug loaded nanoparticle due to the complexity of composition and preparation.
30308299	6	10	dep	nephrotoxicity	1032:1045	arg1	the					1028:1030	the	1028:1030	the	1028:1030	Bio-safety is also a distinguishing feature of GA-ALG@Pt NPs that greatly relief the nephrotoxicity and systematic toxicity of cisplatin.
30308299	0	11	with	nanoparticle	55:66	arg1	ligand					73:78	ligand	73:78	ligand	73:78	A conveniently synthesized Pt (IV) conjugated alginate nanoparticle with ligand self-shielded property for targeting treatment of hepatic carcinoma.
30308299	3	12	theme	self-shielded	538:550	arg1	property					552:559	intelligently ligand self-shielded property	517:559	intelligently ligand self-shielded property in which the internal GA could be induced to expose by initial cellular recognition, resulting in strengthened cellular uptake (20%-30%) and prolonged blood circulation time (3.43 times)	517:746	GA-ALG@Pt NPs were found equipped with intelligently ligand self-shielded property in which the internal GA could be induced to expose by initial cellular recognition, resulting in strengthened cellular uptake (20%-30%) and prolonged blood circulation time (3.43 times).
30308299	5	13	theme	therapeutic	888:898	arg1	agent					900:904	therapeutic agent	888:904	therapeutic agent	888:904	Glutathione-triggered release of therapeutic agent generated satisfactory antitumor effect.
30308299	5	14	theme	Glutathione-triggered	855:875	arg1	release					877:883	Glutathione-triggered release	855:883	Glutathione-triggered release of therapeutic agent	855:904	Glutathione-triggered release of therapeutic agent generated satisfactory antitumor effect.
30308299	6	15	theme	systematic	1051:1060	arg1	toxicity					1062:1069	systematic toxicity	1051:1069	systematic toxicity	1051:1069	Bio-safety is also a distinguishing feature of GA-ALG@Pt NPs that greatly relief the nephrotoxicity and systematic toxicity of cisplatin.
30308299	2	16	theme	GA-ALG	411:416	arg1	NPs					421:423	GA-ALG@Pt NPs	411:423	GA-ALG@Pt NPs	411:423	Here we employed only three ingredients to prepare Pt (IV) prodrug-loaded ligand-induced self-assembled nanoparticles (GA-ALG@Pt NPs) via facile one-pot route for liver tumor treatment.
30308299	2	16	theme	GA-ALG	411:416	arg1	nanoparticles					396:408	ligand-induced self-assembled nanoparticles	366:408	Pt (IV) prodrug-loaded ligand-induced self-assembled nanoparticles (GA-ALG@Pt NPs)	343:424	Here we employed only three ingredients to prepare Pt (IV) prodrug-loaded ligand-induced self-assembled nanoparticles (GA-ALG@Pt NPs) via facile one-pot route for liver tumor treatment.
30308299	7	17	theme	effective	1196:1204	arg1	approach					1206:1213	an effective approach	1193:1213	an effective approach to translational cancer therapy	1193:1245	This conveniently synthesized nanoparticle processes superior targeting capacity and biosecurity, supplying an effective approach to translational cancer therapy in the future.
30308299	0	18	dep	self-shielded	80:92	arg1	IV					31:32	IV	31:32	IV	31:32	A conveniently synthesized Pt (IV) conjugated alginate nanoparticle with ligand self-shielded property for targeting treatment of hepatic carcinoma.
30308299	4	19	theme	tumor	810:814	arg1	selectivity					816:826	tumor selectivity	810:826	Appreciable tumor targeting ability (2 times) and especially tumor selectivity (2.5 times)	749:838	Appreciable tumor targeting ability (2 times) and especially tumor selectivity (2.5 times) were obtained.
30308299	4	19	theme	tumor	810:814	arg1	times					833:837	2.5 times	829:837	2.5 times	829:837	Appreciable tumor targeting ability (2 times) and especially tumor selectivity (2.5 times) were obtained.
30308299	3	20	theme	circulation	718:728	arg1	time					730:733	prolonged blood circulation time	702:733	prolonged blood circulation time (3.43 times)	702:746	GA-ALG@Pt NPs were found equipped with intelligently ligand self-shielded property in which the internal GA could be induced to expose by initial cellular recognition, resulting in strengthened cellular uptake (20%-30%) and prolonged blood circulation time (3.43 times).
30308299	3	20	theme	circulation	718:728	arg1	times					741:745	3.43 times	736:745	3.43 times	736:745	GA-ALG@Pt NPs were found equipped with intelligently ligand self-shielded property in which the internal GA could be induced to expose by initial cellular recognition, resulting in strengthened cellular uptake (20%-30%) and prolonged blood circulation time (3.43 times).
30308299	5	21	theme	agent	900:904	arg1	release					877:883	Glutathione-triggered release	855:883	Glutathione-triggered release of therapeutic agent	855:904	Glutathione-triggered release of therapeutic agent generated satisfactory antitumor effect.
30308299	1	22	theme	major	184:188	arg1	challenge					190:198	a major challenge	182:198	a major challenge for platinum drug loaded nanoparticle due to the complexity of composition and preparation	182:289	The clinical translation remains a major challenge for platinum drug loaded nanoparticle due to the complexity of composition and preparation.
30308299	2	23	theme	one-pot	437:443	arg1	route					445:449	facile one-pot route	430:449	facile one-pot route for liver tumor treatment	430:475	Here we employed only three ingredients to prepare Pt (IV) prodrug-loaded ligand-induced self-assembled nanoparticles (GA-ALG@Pt NPs) via facile one-pot route for liver tumor treatment.
30308299	1	24	theme	preparation	279:289	arg1	complexity					249:258	the complexity	245:258	the complexity of composition and preparation	245:289	The clinical translation remains a major challenge for platinum drug loaded nanoparticle due to the complexity of composition and preparation.
30308299	2	25	theme	self-assembled	381:394	arg1	NPs					421:423	GA-ALG@Pt NPs	411:423	GA-ALG@Pt NPs	411:423	Here we employed only three ingredients to prepare Pt (IV) prodrug-loaded ligand-induced self-assembled nanoparticles (GA-ALG@Pt NPs) via facile one-pot route for liver tumor treatment.
30308299	2	25	theme	self-assembled	381:394	arg1	nanoparticles					396:408	ligand-induced self-assembled nanoparticles	366:408	Pt (IV) prodrug-loaded ligand-induced self-assembled nanoparticles (GA-ALG@Pt NPs)	343:424	Here we employed only three ingredients to prepare Pt (IV) prodrug-loaded ligand-induced self-assembled nanoparticles (GA-ALG@Pt NPs) via facile one-pot route for liver tumor treatment.
30308299	2	26	theme	facile	430:435	arg1	route					445:449	facile one-pot route	430:449	facile one-pot route for liver tumor treatment	430:475	Here we employed only three ingredients to prepare Pt (IV) prodrug-loaded ligand-induced self-assembled nanoparticles (GA-ALG@Pt NPs) via facile one-pot route for liver tumor treatment.
30308299	3	27	dep	found	497:501	arg1	equipped					503:510	equipped	503:510	equipped with intelligently ligand self-shielded property in which the internal GA could be induced to expose by initial cellular recognition, resulting in strengthened cellular uptake (20%-30%) and prolonged blood circulation time (3.43 times)	503:746	GA-ALG@Pt NPs were found equipped with intelligently ligand self-shielded property in which the internal GA could be induced to expose by initial cellular recognition, resulting in strengthened cellular uptake (20%-30%) and prolonged blood circulation time (3.43 times).
30308299	0	28	theme	synthesized	15:25	arg1	Pt					27:28	A conveniently synthesized Pt	0:28	A conveniently synthesized Pt	0:28	A conveniently synthesized Pt (IV) conjugated alginate nanoparticle with ligand self-shielded property for targeting treatment of hepatic carcinoma.
30308299	3	29	theme	prolonged	702:710	arg1	time					730:733	prolonged blood circulation time	702:733	prolonged blood circulation time (3.43 times)	702:746	GA-ALG@Pt NPs were found equipped with intelligently ligand self-shielded property in which the internal GA could be induced to expose by initial cellular recognition, resulting in strengthened cellular uptake (20%-30%) and prolonged blood circulation time (3.43 times).
30308299	3	29	theme	prolonged	702:710	arg1	times					741:745	3.43 times	736:745	3.43 times	736:745	GA-ALG@Pt NPs were found equipped with intelligently ligand self-shielded property in which the internal GA could be induced to expose by initial cellular recognition, resulting in strengthened cellular uptake (20%-30%) and prolonged blood circulation time (3.43 times).
30308299	5	30	theme	antitumor	929:937	arg1	effect					939:944	satisfactory antitumor effect	916:944	satisfactory antitumor effect	916:944	Glutathione-triggered release of therapeutic agent generated satisfactory antitumor effect.
30308299	1	31	theme	platinum	204:211	arg1	nanoparticle					225:236	platinum drug loaded nanoparticle	204:236	platinum drug loaded nanoparticle due to the complexity of composition and preparation	204:289	The clinical translation remains a major challenge for platinum drug loaded nanoparticle due to the complexity of composition and preparation.
30308299	7	32	theme	targeting	1147:1155	arg1	capacity					1157:1164	superior targeting capacity	1138:1164	superior targeting capacity	1138:1164	This conveniently synthesized nanoparticle processes superior targeting capacity and biosecurity, supplying an effective approach to translational cancer therapy in the future.
30308299	3	33	theme	blood	712:716	arg1	time					730:733	prolonged blood circulation time	702:733	prolonged blood circulation time (3.43 times)	702:746	GA-ALG@Pt NPs were found equipped with intelligently ligand self-shielded property in which the internal GA could be induced to expose by initial cellular recognition, resulting in strengthened cellular uptake (20%-30%) and prolonged blood circulation time (3.43 times).
30308299	3	33	theme	blood	712:716	arg1	times					741:745	3.43 times	736:745	3.43 times	736:745	GA-ALG@Pt NPs were found equipped with intelligently ligand self-shielded property in which the internal GA could be induced to expose by initial cellular recognition, resulting in strengthened cellular uptake (20%-30%) and prolonged blood circulation time (3.43 times).
30308299	0	34	theme	carcinoma	138:146	arg1	treatment					117:125	treatment	117:125	treatment of hepatic carcinoma	117:146	A conveniently synthesized Pt (IV) conjugated alginate nanoparticle with ligand self-shielded property for targeting treatment of hepatic carcinoma.
30308299	1	35	theme	drug	213:216	arg1	nanoparticle					225:236	platinum drug loaded nanoparticle	204:236	platinum drug loaded nanoparticle due to the complexity of composition and preparation	204:289	The clinical translation remains a major challenge for platinum drug loaded nanoparticle due to the complexity of composition and preparation.
30308299	2	36	theme	Pt	418:419	arg1	NPs					421:423	GA-ALG@Pt NPs	411:423	GA-ALG@Pt NPs	411:423	Here we employed only three ingredients to prepare Pt (IV) prodrug-loaded ligand-induced self-assembled nanoparticles (GA-ALG@Pt NPs) via facile one-pot route for liver tumor treatment.
30308299	2	36	theme	Pt	418:419	arg1	nanoparticles					396:408	ligand-induced self-assembled nanoparticles	366:408	Pt (IV) prodrug-loaded ligand-induced self-assembled nanoparticles (GA-ALG@Pt NPs)	343:424	Here we employed only three ingredients to prepare Pt (IV) prodrug-loaded ligand-induced self-assembled nanoparticles (GA-ALG@Pt NPs) via facile one-pot route for liver tumor treatment.
30308299	2	37	theme	tumor	461:465	arg1	treatment					467:475	liver tumor treatment	455:475	liver tumor treatment	455:475	Here we employed only three ingredients to prepare Pt (IV) prodrug-loaded ligand-induced self-assembled nanoparticles (GA-ALG@Pt NPs) via facile one-pot route for liver tumor treatment.
30308299	0	38	theme	hepatic	130:136	arg1	carcinoma					138:146	hepatic carcinoma	130:146	hepatic carcinoma	130:146	A conveniently synthesized Pt (IV) conjugated alginate nanoparticle with ligand self-shielded property for targeting treatment of hepatic carcinoma.
30308299	3	39	theme	initial	616:622	arg1	recognition					633:643	initial cellular recognition	616:643	initial cellular recognition	616:643	GA-ALG@Pt NPs were found equipped with intelligently ligand self-shielded property in which the internal GA could be induced to expose by initial cellular recognition, resulting in strengthened cellular uptake (20%-30%) and prolonged blood circulation time (3.43 times).
30308299	1	40	theme	loaded	218:223	arg1	nanoparticle					225:236	platinum drug loaded nanoparticle	204:236	platinum drug loaded nanoparticle due to the complexity of composition and preparation	204:289	The clinical translation remains a major challenge for platinum drug loaded nanoparticle due to the complexity of composition and preparation.
30308299	2	41	theme	liver	455:459	arg1	treatment					467:475	liver tumor treatment	455:475	liver tumor treatment	455:475	Here we employed only three ingredients to prepare Pt (IV) prodrug-loaded ligand-induced self-assembled nanoparticles (GA-ALG@Pt NPs) via facile one-pot route for liver tumor treatment.
30308299	6	42	theme	NPs	1004:1006	arg1	feature					983:989	a distinguishing feature	966:989	a distinguishing feature of GA-ALG@Pt NPs that greatly relief the nephrotoxicity and systematic toxicity of cisplatin	966:1082	Bio-safety is also a distinguishing feature of GA-ALG@Pt NPs that greatly relief the nephrotoxicity and systematic toxicity of cisplatin.
30308299	6	42	theme	NPs	1004:1006	arg1	Bio-safety					947:956	Bio-safety	947:956	Bio-safety	947:956	Bio-safety is also a distinguishing feature of GA-ALG@Pt NPs that greatly relief the nephrotoxicity and systematic toxicity of cisplatin.
30308299	0	43	theme	alginate	46:53	arg1	nanoparticle					55:66	conjugated alginate nanoparticle	35:66	conjugated alginate nanoparticle with ligand	35:78	A conveniently synthesized Pt (IV) conjugated alginate nanoparticle with ligand self-shielded property for targeting treatment of hepatic carcinoma.
30308299	3	44	theme	internal	574:581	arg1	GA					583:584	the internal GA	570:584	the internal GA	570:584	GA-ALG@Pt NPs were found equipped with intelligently ligand self-shielded property in which the internal GA could be induced to expose by initial cellular recognition, resulting in strengthened cellular uptake (20%-30%) and prolonged blood circulation time (3.43 times).
30308299	3	45	theme	@	484:484	arg1	NPs					488:490	GA-ALG@Pt NPs	478:490	GA-ALG@Pt NPs	478:490	GA-ALG@Pt NPs were found equipped with intelligently ligand self-shielded property in which the internal GA could be induced to expose by initial cellular recognition, resulting in strengthened cellular uptake (20%-30%) and prolonged blood circulation time (3.43 times).
30308299	7	46	theme	superior	1138:1145	arg1	capacity					1157:1164	superior targeting capacity	1138:1164	superior targeting capacity	1138:1164	This conveniently synthesized nanoparticle processes superior targeting capacity and biosecurity, supplying an effective approach to translational cancer therapy in the future.
30308299	3	47	theme	strengthened	659:670	arg1	%					695:695	20%-30%	689:695	20%-30%	689:695	GA-ALG@Pt NPs were found equipped with intelligently ligand self-shielded property in which the internal GA could be induced to expose by initial cellular recognition, resulting in strengthened cellular uptake (20%-30%) and prolonged blood circulation time (3.43 times).
30308299	3	47	theme	strengthened	659:670	arg1	uptake					681:686	strengthened cellular uptake	659:686	strengthened cellular uptake (20%-30%)	659:696	GA-ALG@Pt NPs were found equipped with intelligently ligand self-shielded property in which the internal GA could be induced to expose by initial cellular recognition, resulting in strengthened cellular uptake (20%-30%) and prolonged blood circulation time (3.43 times).
30308299	3	48	theme	Pt	485:486	arg1	NPs					488:490	GA-ALG@Pt NPs	478:490	GA-ALG@Pt NPs	478:490	GA-ALG@Pt NPs were found equipped with intelligently ligand self-shielded property in which the internal GA could be induced to expose by initial cellular recognition, resulting in strengthened cellular uptake (20%-30%) and prolonged blood circulation time (3.43 times).
30308299	3	49	theme	cellular	672:679	arg1	%					695:695	20%-30%	689:695	20%-30%	689:695	GA-ALG@Pt NPs were found equipped with intelligently ligand self-shielded property in which the internal GA could be induced to expose by initial cellular recognition, resulting in strengthened cellular uptake (20%-30%) and prolonged blood circulation time (3.43 times).
30308299	3	49	theme	cellular	672:679	arg1	uptake					681:686	strengthened cellular uptake	659:686	strengthened cellular uptake (20%-30%)	659:696	GA-ALG@Pt NPs were found equipped with intelligently ligand self-shielded property in which the internal GA could be induced to expose by initial cellular recognition, resulting in strengthened cellular uptake (20%-30%) and prolonged blood circulation time (3.43 times).
30308299	4	50	theme	Appreciable	749:759	arg1	ability					777:783	Appreciable tumor targeting ability	749:783	Appreciable tumor targeting ability (2 times) and especially tumor selectivity (2.5 times)	749:838	Appreciable tumor targeting ability (2 times) and especially tumor selectivity (2.5 times) were obtained.
30308299	4	50	theme	Appreciable	749:759	arg1	times					788:792	2 times	786:792	2 times	786:792	Appreciable tumor targeting ability (2 times) and especially tumor selectivity (2.5 times) were obtained.
30308299	6	51	theme	@	1000:1000	arg1	NPs					1004:1006	GA-ALG@Pt NPs	994:1006	GA-ALG@Pt NPs	994:1006	Bio-safety is also a distinguishing feature of GA-ALG@Pt NPs that greatly relief the nephrotoxicity and systematic toxicity of cisplatin.
30308299	5	52	theme	satisfactory	916:927	arg1	effect					939:944	satisfactory antitumor effect	916:944	satisfactory antitumor effect	916:944	Glutathione-triggered release of therapeutic agent generated satisfactory antitumor effect.
30308299	6	53	theme	Pt	1001:1002	arg1	NPs					1004:1006	GA-ALG@Pt NPs	994:1006	GA-ALG@Pt NPs	994:1006	Bio-safety is also a distinguishing feature of GA-ALG@Pt NPs that greatly relief the nephrotoxicity and systematic toxicity of cisplatin.
30308299	3	54	theme	intelligently	517:529	arg1	property					552:559	intelligently ligand self-shielded property	517:559	intelligently ligand self-shielded property in which the internal GA could be induced to expose by initial cellular recognition, resulting in strengthened cellular uptake (20%-30%) and prolonged blood circulation time (3.43 times)	517:746	GA-ALG@Pt NPs were found equipped with intelligently ligand self-shielded property in which the internal GA could be induced to expose by initial cellular recognition, resulting in strengthened cellular uptake (20%-30%) and prolonged blood circulation time (3.43 times).
30308299	3	55	theme	GA-ALG	478:483	arg1	NPs					488:490	GA-ALG@Pt NPs	478:490	GA-ALG@Pt NPs	478:490	GA-ALG@Pt NPs were found equipped with intelligently ligand self-shielded property in which the internal GA could be induced to expose by initial cellular recognition, resulting in strengthened cellular uptake (20%-30%) and prolonged blood circulation time (3.43 times).
30308299	1	56	theme	due	238:240	arg1	nanoparticle					225:236	platinum drug loaded nanoparticle	204:236	platinum drug loaded nanoparticle due to the complexity of composition and preparation	204:289	The clinical translation remains a major challenge for platinum drug loaded nanoparticle due to the complexity of composition and preparation.
30308299	2	57	theme	@	417:417	arg1	NPs					421:423	GA-ALG@Pt NPs	411:423	GA-ALG@Pt NPs	411:423	Here we employed only three ingredients to prepare Pt (IV) prodrug-loaded ligand-induced self-assembled nanoparticles (GA-ALG@Pt NPs) via facile one-pot route for liver tumor treatment.
30308299	2	57	theme	@	417:417	arg1	nanoparticles					396:408	ligand-induced self-assembled nanoparticles	366:408	Pt (IV) prodrug-loaded ligand-induced self-assembled nanoparticles (GA-ALG@Pt NPs)	343:424	Here we employed only three ingredients to prepare Pt (IV) prodrug-loaded ligand-induced self-assembled nanoparticles (GA-ALG@Pt NPs) via facile one-pot route for liver tumor treatment.
30308299	4	58	theme	targeting	767:775	arg1	ability					777:783	Appreciable tumor targeting ability	749:783	Appreciable tumor targeting ability (2 times) and especially tumor selectivity (2.5 times)	749:838	Appreciable tumor targeting ability (2 times) and especially tumor selectivity (2.5 times) were obtained.
30308299	4	58	theme	targeting	767:775	arg1	times					788:792	2 times	786:792	2 times	786:792	Appreciable tumor targeting ability (2 times) and especially tumor selectivity (2.5 times) were obtained.
30308299	2	59	theme	ligand-induced	366:379	arg1	NPs					421:423	GA-ALG@Pt NPs	411:423	GA-ALG@Pt NPs	411:423	Here we employed only three ingredients to prepare Pt (IV) prodrug-loaded ligand-induced self-assembled nanoparticles (GA-ALG@Pt NPs) via facile one-pot route for liver tumor treatment.
30308299	2	59	theme	ligand-induced	366:379	arg1	nanoparticles					396:408	ligand-induced self-assembled nanoparticles	366:408	Pt (IV) prodrug-loaded ligand-induced self-assembled nanoparticles (GA-ALG@Pt NPs)	343:424	Here we employed only three ingredients to prepare Pt (IV) prodrug-loaded ligand-induced self-assembled nanoparticles (GA-ALG@Pt NPs) via facile one-pot route for liver tumor treatment.
30308299	6	60	theme	GA-ALG	994:999	arg1	NPs					1004:1006	GA-ALG@Pt NPs	994:1006	GA-ALG@Pt NPs	994:1006	Bio-safety is also a distinguishing feature of GA-ALG@Pt NPs that greatly relief the nephrotoxicity and systematic toxicity of cisplatin.
30308299	0	61	theme	conjugated	35:44	arg1	nanoparticle					55:66	conjugated alginate nanoparticle	35:66	conjugated alginate nanoparticle with ligand	35:78	A conveniently synthesized Pt (IV) conjugated alginate nanoparticle with ligand self-shielded property for targeting treatment of hepatic carcinoma.
30308299	4	62	theme	tumor	761:765	arg1	ability					777:783	Appreciable tumor targeting ability	749:783	Appreciable tumor targeting ability (2 times) and especially tumor selectivity (2.5 times)	749:838	Appreciable tumor targeting ability (2 times) and especially tumor selectivity (2.5 times) were obtained.
30308299	4	62	theme	tumor	761:765	arg1	times					788:792	2 times	786:792	2 times	786:792	Appreciable tumor targeting ability (2 times) and especially tumor selectivity (2.5 times) were obtained.
30308299	2	63	theme	prodrug-loaded	351:364	arg1	NPs					421:423	GA-ALG@Pt NPs	411:423	GA-ALG@Pt NPs	411:423	Here we employed only three ingredients to prepare Pt (IV) prodrug-loaded ligand-induced self-assembled nanoparticles (GA-ALG@Pt NPs) via facile one-pot route for liver tumor treatment.
30308299	2	63	theme	prodrug-loaded	351:364	arg1	nanoparticles					396:408	ligand-induced self-assembled nanoparticles	366:408	Pt (IV) prodrug-loaded ligand-induced self-assembled nanoparticles (GA-ALG@Pt NPs)	343:424	Here we employed only three ingredients to prepare Pt (IV) prodrug-loaded ligand-induced self-assembled nanoparticles (GA-ALG@Pt NPs) via facile one-pot route for liver tumor treatment.
31184567	15	0	theme	Ornithinibacillus	1546:1562	arg1	description					1521:1531	An emended description	1510:1531	An emended description of the genus Ornithinibacillus	1510:1562	An emended description of the genus Ornithinibacillus is presented.
31184567	8	1	theme	respiratory	920:930	arg1	menaquinone-7					944:956	menaquinone-7	944:956	menaquinone-7 (MK-7)	944:963	The major respiratory quinone was menaquinone-7 (MK-7).
31184567	8	1	theme	respiratory	920:930	arg1	quinone					932:938	The major respiratory quinone	910:938	The major respiratory quinone	910:938	The major respiratory quinone was menaquinone-7 (MK-7).
31184567	1	2	theme	Ornithinibacillus	140:156	arg1	description					115:125	emended description	107:125	emended description of the genus Ornithinibacillus	107:156	nov., a halophilic bacterium isolated from lake sediment, and emended description of the genus Ornithinibacillus.
31184567	1	2	theme	Ornithinibacillus	140:156	arg1	bacterium					64:72	a halophilic bacterium	51:72	a halophilic bacterium isolated from lake sediment	51:100	nov., a halophilic bacterium isolated from lake sediment, and emended description of the genus Ornithinibacillus.
31184567	1	2	theme	Ornithinibacillus	140:156	arg1	nov.					45:48	nov.	45:48	nov.	45:48	nov., a halophilic bacterium isolated from lake sediment, and emended description of the genus Ornithinibacillus.
31184567	7	3	theme	amino	871:875	arg1	type					882:885	The peptidoglycan amino acid type	853:885	The peptidoglycan amino acid type	853:885	The peptidoglycan amino acid type was A4α (l-Lys-d-Asp).
31184567	7	3	theme	amino	871:875	arg1	A4α					891:893	A4α	891:893	A4α (l-Lys-d-Asp)	891:907	The peptidoglycan amino acid type was A4α (l-Lys-d-Asp).
31184567	2	4	dep	Gram-stain-positive	161:179	arg1	aerobic					191:197	aerobic	191:197	aerobic	191:197	A Gram-stain-positive, strictly aerobic, motile and rod-shaped bacterium, designated strain LJ137T, was isolated from the sediment of Taihu Lake in China.
31184567	12	5	theme	gellani	1421:1427	arg1	strain					1378:1383	this strain	1373:1383	this strain	1373:1383	Based on the phenotypic, chemotaxonomic, phylogenetic and genome sequence characteristics of this strain, a novel species, Ornithinibacillus gellani sp.
31184567	12	5	theme	gellani	1421:1427	arg1	sp					1429:1430	Ornithinibacillus gellani sp	1403:1430	Ornithinibacillus gellani sp	1403:1430	Based on the phenotypic, chemotaxonomic, phylogenetic and genome sequence characteristics of this strain, a novel species, Ornithinibacillus gellani sp.
31184567	9	6	theme	polar	970:974	arg1	lipids					976:981	The polar lipids	966:981	The polar lipids of strain LJ137T	966:998	The polar lipids of strain LJ137T contained diphosphatidylglycerol, phosphatidylglycerol, three unidentified phospholipids, two aminophospholipids and one unidentified lipid.
31184567	8	7	theme	major	914:918	arg1	menaquinone-7					944:956	menaquinone-7	944:956	menaquinone-7 (MK-7)	944:963	The major respiratory quinone was menaquinone-7 (MK-7).
31184567	8	7	theme	major	914:918	arg1	quinone					932:938	The major respiratory quinone	910:938	The major respiratory quinone	910:938	The major respiratory quinone was menaquinone-7 (MK-7).
31184567	4	8	theme	2.5 	442:445	arg1	%					446:446	%	446:446	%	446:446	Strain LJ137T grew optimally at pH 7.5, at 37 °C and with 2.5 % (w/v) NaCl.
31184567	9	9	theme	unidentified	1121:1132	arg1	lipid					1134:1138	one unidentified lipid	1117:1138	one unidentified lipid	1117:1138	The polar lipids of strain LJ137T contained diphosphatidylglycerol, phosphatidylglycerol, three unidentified phospholipids, two aminophospholipids and one unidentified lipid.
31184567	14	10	theme	1.13678T=NBRC	1486:1498	arg1	LJ137T					1471:1476	LJ137T	1471:1476	LJ137T (=CGMCC 1.13678T=NBRC 113552T)	1471:1507	The type strain is LJ137T (=CGMCC 1.13678T=NBRC 113552T).
31184567	14	10	theme	1.13678T=NBRC	1486:1498	arg1	113552T					1500:1506	=CGMCC 1.13678T=NBRC 113552T	1479:1506	=CGMCC 1.13678T=NBRC 113552T	1479:1506	The type strain is LJ137T (=CGMCC 1.13678T=NBRC 113552T).
31184567	1	11	theme	halophilic	53:62	arg1	bacterium					64:72	a halophilic bacterium	51:72	a halophilic bacterium isolated from lake sediment	51:100	nov., a halophilic bacterium isolated from lake sediment, and emended description of the genus Ornithinibacillus.
31184567	7	12	theme	acid	877:880	arg1	type					882:885	The peptidoglycan amino acid type	853:885	The peptidoglycan amino acid type	853:885	The peptidoglycan amino acid type was A4α (l-Lys-d-Asp).
31184567	7	12	theme	acid	877:880	arg1	A4α					891:893	A4α	891:893	A4α (l-Lys-d-Asp)	891:907	The peptidoglycan amino acid type was A4α (l-Lys-d-Asp).
31184567	10	13	theme	40.4 mol	1180:1187	arg1	%					1188:1188	40.4 mol%	1180:1188	40.4 mol%	1180:1188	The G+C content of the genomic DNA was 40.4 mol%.
31184567	10	13	theme	40.4 mol	1180:1187	arg1	content					1149:1155	The G+C content	1141:1155	The G+C content of the genomic DNA	1141:1174	The G+C content of the genomic DNA was 40.4 mol%.
31184567	14	14	theme	=CGMCC	1479:1484	arg1	LJ137T					1471:1476	LJ137T	1471:1476	LJ137T (=CGMCC 1.13678T=NBRC 113552T)	1471:1507	The type strain is LJ137T (=CGMCC 1.13678T=NBRC 113552T).
31184567	14	14	theme	=CGMCC	1479:1484	arg1	113552T					1500:1506	=CGMCC 1.13678T=NBRC 113552T	1479:1506	=CGMCC 1.13678T=NBRC 113552T	1479:1506	The type strain is LJ137T (=CGMCC 1.13678T=NBRC 113552T).
31184567	12	15	theme	strain	1378:1383	arg1	characteristics					1354:1368	the phenotypic, chemotaxonomic, phylogenetic and genome sequence characteristics	1289:1368	the phenotypic, chemotaxonomic, phylogenetic and genome sequence characteristics of this strain, a novel species, Ornithinibacillus gellani sp	1289:1430	Based on the phenotypic, chemotaxonomic, phylogenetic and genome sequence characteristics of this strain, a novel species, Ornithinibacillus gellani sp.
31184567	6	16	theme	KCTC	690:693	arg1	13822T					695:700	KCTC 13822T	690:700	Ornithinibacillus halophilus KCTC 13822T	661:700	The closest phylogenetic neighbours were Ornithinibacillus halophilus KCTC 13822T, Ornithinibacillus salinisoli LCB256T and Oceanobacillus limi KCTC 13823T, with 95.2, 96.5 and 95.6 % 16S rRNA gene sequence similarity, respectively.
31184567	9	17	theme	strain	986:991	arg1	LJ137T					993:998	strain LJ137T	986:998	strain LJ137T	986:998	The polar lipids of strain LJ137T contained diphosphatidylglycerol, phosphatidylglycerol, three unidentified phospholipids, two aminophospholipids and one unidentified lipid.
31184567	9	18	theme	unidentified	1062:1073	arg1	phospholipids					1075:1087	three unidentified phospholipids	1056:1087	three unidentified phospholipids	1056:1087	The polar lipids of strain LJ137T contained diphosphatidylglycerol, phosphatidylglycerol, three unidentified phospholipids, two aminophospholipids and one unidentified lipid.
31184567	9	18	theme	unidentified	1062:1073	arg1	aminophospholipids					1094:1111	two aminophospholipids	1090:1111	two aminophospholipids	1090:1111	The polar lipids of strain LJ137T contained diphosphatidylglycerol, phosphatidylglycerol, three unidentified phospholipids, two aminophospholipids and one unidentified lipid.
31184567	10	19	theme	G+C	1145:1147	arg1	%					1188:1188	40.4 mol%	1180:1188	40.4 mol%	1180:1188	The G+C content of the genomic DNA was 40.4 mol%.
31184567	10	19	theme	G+C	1145:1147	arg1	content					1149:1155	The G+C content	1141:1155	The G+C content of the genomic DNA	1141:1174	The G+C content of the genomic DNA was 40.4 mol%.
31184567	3	20	theme	taxonomic	364:372	arg1	position					374:381	its taxonomic position	360:381	its taxonomic position	360:381	A polyphasic approach was used to investigate its taxonomic position.
31184567	4	21	theme	%	446:446	arg1	NaCl					454:457	2.5 % (w/v) NaCl	442:457	2.5 % (w/v) NaCl	442:457	Strain LJ137T grew optimally at pH 7.5, at 37 °C and with 2.5 % (w/v) NaCl.
31184567	6	22	dep	Ornithinibacillus	661:677	arg1	halophilus					679:688	Ornithinibacillus halophilus KCTC 13822T	661:700	Ornithinibacillus halophilus KCTC 13822T	661:700	The closest phylogenetic neighbours were Ornithinibacillus halophilus KCTC 13822T, Ornithinibacillus salinisoli LCB256T and Oceanobacillus limi KCTC 13823T, with 95.2, 96.5 and 95.6 % 16S rRNA gene sequence similarity, respectively.
31184567	6	22	dep	Ornithinibacillus	661:677	arg1	13823T					769:774	KCTC 13823T	764:774	KCTC 13823T	764:774	The closest phylogenetic neighbours were Ornithinibacillus halophilus KCTC 13822T, Ornithinibacillus salinisoli LCB256T and Oceanobacillus limi KCTC 13823T, with 95.2, 96.5 and 95.6 % 16S rRNA gene sequence similarity, respectively.
31184567	6	22	dep	Ornithinibacillus	661:677	arg1	13822T					695:700	KCTC 13822T	690:700	Ornithinibacillus halophilus KCTC 13822T	661:700	The closest phylogenetic neighbours were Ornithinibacillus halophilus KCTC 13822T, Ornithinibacillus salinisoli LCB256T and Oceanobacillus limi KCTC 13823T, with 95.2, 96.5 and 95.6 % 16S rRNA gene sequence similarity, respectively.
31184567	3	23	used	used	340:343	arg2	approach					327:334	A polyphasic approach	314:334	A polyphasic approach	314:334	A polyphasic approach was used to investigate its taxonomic position.
31184567	5	24	theme	strain	529:534	arg1	LJ137T					536:541	strain LJ137T	529:541	strain LJ137T	529:541	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain LJ137T was most closely related to the genera Ornithinibacillus and Oceanobacillus.
31184567	2	25	theme	Lake	299:302	arg1	sediment					281:288	the sediment	277:288	the sediment of Taihu Lake in China	277:311	A Gram-stain-positive, strictly aerobic, motile and rod-shaped bacterium, designated strain LJ137T, was isolated from the sediment of Taihu Lake in China.
31184567	12	26	theme	chemotaxonomic	1305:1318	arg1	characteristics					1354:1368	the phenotypic, chemotaxonomic, phylogenetic and genome sequence characteristics	1289:1368	the phenotypic, chemotaxonomic, phylogenetic and genome sequence characteristics of this strain, a novel species, Ornithinibacillus gellani sp	1289:1430	Based on the phenotypic, chemotaxonomic, phylogenetic and genome sequence characteristics of this strain, a novel species, Ornithinibacillus gellani sp.
31184567	0	27	theme	gellani	33:39	arg1	Description					0:10	Description	0:10	Description of Ornithinibacillus gellani	0:39	Description of Ornithinibacillus gellani sp.
31184567	4	28	dep	%	446:446	arg1	w/v					449:451	w/v	449:451	w/v	449:451	Strain LJ137T grew optimally at pH 7.5, at 37 °C and with 2.5 % (w/v) NaCl.
31184567	11	29	theme	fatty	1213:1217	arg1	anteiso-C15 					1230:1241	anteiso-C15 	1230:1241	anteiso-C15 	1230:1241	The dominant cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C15 : 0.
31184567	11	29	theme	fatty	1213:1217	arg1	acids					1219:1223	The dominant cellular fatty acids	1191:1223	The dominant cellular fatty acids	1191:1223	The dominant cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C15 : 0.
31184567	0	30	dep	sp	41:42	arg1	Description					0:10	Description	0:10	Description of Ornithinibacillus gellani	0:39	Description of Ornithinibacillus gellani sp.
31184567	12	31	theme	sequence	1345:1352	arg1	characteristics					1354:1368	the phenotypic, chemotaxonomic, phylogenetic and genome sequence characteristics	1289:1368	the phenotypic, chemotaxonomic, phylogenetic and genome sequence characteristics of this strain, a novel species, Ornithinibacillus gellani sp	1289:1430	Based on the phenotypic, chemotaxonomic, phylogenetic and genome sequence characteristics of this strain, a novel species, Ornithinibacillus gellani sp.
31184567	1	32	theme	lake	88:91	arg1	sediment					93:100	lake sediment	88:100	lake sediment	88:100	nov., a halophilic bacterium isolated from lake sediment, and emended description of the genus Ornithinibacillus.
31184567	1	33	attach	isolated	74:81	arg1	sediment					93:100	lake sediment	88:100	lake sediment	88:100	nov., a halophilic bacterium isolated from lake sediment, and emended description of the genus Ornithinibacillus.
31184567	1	33	attach	isolated	74:81	arg2	bacterium					64:72	a halophilic bacterium	51:72	a halophilic bacterium isolated from lake sediment	51:100	nov., a halophilic bacterium isolated from lake sediment, and emended description of the genus Ornithinibacillus.
31184567	6	34	theme	phylogenetic	632:643	arg1	neighbours					645:654	The closest phylogenetic neighbours	620:654	The closest phylogenetic neighbours	620:654	The closest phylogenetic neighbours were Ornithinibacillus halophilus KCTC 13822T, Ornithinibacillus salinisoli LCB256T and Oceanobacillus limi KCTC 13823T, with 95.2, 96.5 and 95.6 % 16S rRNA gene sequence similarity, respectively.
31184567	6	34	theme	phylogenetic	632:643	arg1	Ornithinibacillus					661:677	Ornithinibacillus	661:677	Ornithinibacillus	661:677	The closest phylogenetic neighbours were Ornithinibacillus halophilus KCTC 13822T, Ornithinibacillus salinisoli LCB256T and Oceanobacillus limi KCTC 13823T, with 95.2, 96.5 and 95.6 % 16S rRNA gene sequence similarity, respectively.
31184567	5	35	dep	genera	575:580	arg1	Oceanobacillus					604:617	Oceanobacillus	604:617	Oceanobacillus	604:617	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain LJ137T was most closely related to the genera Ornithinibacillus and Oceanobacillus.
31184567	5	35	dep	genera	575:580	arg1	Ornithinibacillus					582:598	Ornithinibacillus	582:598	Ornithinibacillus	582:598	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain LJ137T was most closely related to the genera Ornithinibacillus and Oceanobacillus.
31184567	11	36	theme	dominant	1195:1202	arg1	anteiso-C15 					1230:1241	anteiso-C15 	1230:1241	anteiso-C15 	1230:1241	The dominant cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C15 : 0.
31184567	11	36	theme	dominant	1195:1202	arg1	acids					1219:1223	The dominant cellular fatty acids	1191:1223	The dominant cellular fatty acids	1191:1223	The dominant cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C15 : 0.
31184567	6	37	theme	closest	624:630	arg1	neighbours					645:654	The closest phylogenetic neighbours	620:654	The closest phylogenetic neighbours	620:654	The closest phylogenetic neighbours were Ornithinibacillus halophilus KCTC 13822T, Ornithinibacillus salinisoli LCB256T and Oceanobacillus limi KCTC 13823T, with 95.2, 96.5 and 95.6 % 16S rRNA gene sequence similarity, respectively.
31184567	6	37	theme	closest	624:630	arg1	Ornithinibacillus					661:677	Ornithinibacillus	661:677	Ornithinibacillus	661:677	The closest phylogenetic neighbours were Ornithinibacillus halophilus KCTC 13822T, Ornithinibacillus salinisoli LCB256T and Oceanobacillus limi KCTC 13823T, with 95.2, 96.5 and 95.6 % 16S rRNA gene sequence similarity, respectively.
31184567	15	38	theme	emended	1513:1519	arg1	description					1521:1531	An emended description	1510:1531	An emended description of the genus Ornithinibacillus	1510:1562	An emended description of the genus Ornithinibacillus is presented.
31184567	0	39	theme	Ornithinibacillus	15:31	arg1	gellani					33:39	Ornithinibacillus gellani	15:39	Ornithinibacillus gellani	15:39	Description of Ornithinibacillus gellani sp.
31184567	6	40	dep	similarity	827:836	arg1	%					802:802	%	802:802	%	802:802	The closest phylogenetic neighbours were Ornithinibacillus halophilus KCTC 13822T, Ornithinibacillus salinisoli LCB256T and Oceanobacillus limi KCTC 13823T, with 95.2, 96.5 and 95.6 % 16S rRNA gene sequence similarity, respectively.
31184567	11	41	theme	cellular	1204:1211	arg1	anteiso-C15 					1230:1241	anteiso-C15 	1230:1241	anteiso-C15 	1230:1241	The dominant cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C15 : 0.
31184567	11	41	theme	cellular	1204:1211	arg1	acids					1219:1223	The dominant cellular fatty acids	1191:1223	The dominant cellular fatty acids	1191:1223	The dominant cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C15 : 0.
31184567	2	42	theme	Gram-stain-positive	161:179	arg1	bacterium					222:230	A Gram-stain-positive, strictly aerobic, motile and rod-shaped bacterium	159:230	A Gram-stain-positive, strictly aerobic, motile and rod-shaped bacterium	159:230	A Gram-stain-positive, strictly aerobic, motile and rod-shaped bacterium, designated strain LJ137T, was isolated from the sediment of Taihu Lake in China.
31184567	12	43	theme	phenotypic	1293:1302	arg1	characteristics					1354:1368	the phenotypic, chemotaxonomic, phylogenetic and genome sequence characteristics	1289:1368	the phenotypic, chemotaxonomic, phylogenetic and genome sequence characteristics of this strain, a novel species, Ornithinibacillus gellani sp	1289:1430	Based on the phenotypic, chemotaxonomic, phylogenetic and genome sequence characteristics of this strain, a novel species, Ornithinibacillus gellani sp.
31184567	5	44	theme	16S	491:493	arg1	sequences					505:513	16S rRNA gene sequences	491:513	16S rRNA gene sequences	491:513	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain LJ137T was most closely related to the genera Ornithinibacillus and Oceanobacillus.
31184567	6	45	dep	%	802:802	arg1	96.5					788:791	96.5	788:791	96.5	788:791	The closest phylogenetic neighbours were Ornithinibacillus halophilus KCTC 13822T, Ornithinibacillus salinisoli LCB256T and Oceanobacillus limi KCTC 13823T, with 95.2, 96.5 and 95.6 % 16S rRNA gene sequence similarity, respectively.
31184567	6	45	dep	%	802:802	arg1	95.6 					797:801	95.6 	797:801	95.6 	797:801	The closest phylogenetic neighbours were Ornithinibacillus halophilus KCTC 13822T, Ornithinibacillus salinisoli LCB256T and Oceanobacillus limi KCTC 13823T, with 95.2, 96.5 and 95.6 % 16S rRNA gene sequence similarity, respectively.
31184567	6	46	theme	sequence	818:825	arg1	similarity					827:836	95.2, 96.5 and 95.6 % 16S rRNA gene sequence similarity	782:836	95.2, 96.5 and 95.6 % 16S rRNA gene sequence similarity	782:836	The closest phylogenetic neighbours were Ornithinibacillus halophilus KCTC 13822T, Ornithinibacillus salinisoli LCB256T and Oceanobacillus limi KCTC 13823T, with 95.2, 96.5 and 95.6 % 16S rRNA gene sequence similarity, respectively.
31184567	5	47	theme	rRNA	495:498	arg1	sequences					505:513	16S rRNA gene sequences	491:513	16S rRNA gene sequences	491:513	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain LJ137T was most closely related to the genera Ornithinibacillus and Oceanobacillus.
31184567	12	48	dep	sequence	1345:1352	arg1	genome					1338:1343	genome	1338:1343	genome	1338:1343	Based on the phenotypic, chemotaxonomic, phylogenetic and genome sequence characteristics of this strain, a novel species, Ornithinibacillus gellani sp.
31184567	6	49	theme	rRNA	808:811	arg1	similarity					827:836	95.2, 96.5 and 95.6 % 16S rRNA gene sequence similarity	782:836	95.2, 96.5 and 95.6 % 16S rRNA gene sequence similarity	782:836	The closest phylogenetic neighbours were Ornithinibacillus halophilus KCTC 13822T, Ornithinibacillus salinisoli LCB256T and Oceanobacillus limi KCTC 13823T, with 95.2, 96.5 and 95.6 % 16S rRNA gene sequence similarity, respectively.
31184567	2	50	attach	isolated	263:270	arg1	sediment					281:288	the sediment	277:288	the sediment of Taihu Lake in China	277:311	A Gram-stain-positive, strictly aerobic, motile and rod-shaped bacterium, designated strain LJ137T, was isolated from the sediment of Taihu Lake in China.
31184567	2	50	attach	isolated	263:270	arg2	bacterium					222:230	A Gram-stain-positive, strictly aerobic, motile and rod-shaped bacterium	159:230	A Gram-stain-positive, strictly aerobic, motile and rod-shaped bacterium	159:230	A Gram-stain-positive, strictly aerobic, motile and rod-shaped bacterium, designated strain LJ137T, was isolated from the sediment of Taihu Lake in China.
31184567	15	51	theme	genus	1540:1544	arg1	Ornithinibacillus					1546:1562	the genus Ornithinibacillus	1536:1562	the genus Ornithinibacillus	1536:1562	An emended description of the genus Ornithinibacillus is presented.
31184567	6	52	theme	gene	813:816	arg1	similarity					827:836	95.2, 96.5 and 95.6 % 16S rRNA gene sequence similarity	782:836	95.2, 96.5 and 95.6 % 16S rRNA gene sequence similarity	782:836	The closest phylogenetic neighbours were Ornithinibacillus halophilus KCTC 13822T, Ornithinibacillus salinisoli LCB256T and Oceanobacillus limi KCTC 13823T, with 95.2, 96.5 and 95.6 % 16S rRNA gene sequence similarity, respectively.
31184567	2	53	theme	rod-shaped	211:220	arg1	bacterium					222:230	A Gram-stain-positive, strictly aerobic, motile and rod-shaped bacterium	159:230	A Gram-stain-positive, strictly aerobic, motile and rod-shaped bacterium	159:230	A Gram-stain-positive, strictly aerobic, motile and rod-shaped bacterium, designated strain LJ137T, was isolated from the sediment of Taihu Lake in China.
31184567	5	54	theme	gene	500:503	arg1	sequences					505:513	16S rRNA gene sequences	491:513	16S rRNA gene sequences	491:513	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain LJ137T was most closely related to the genera Ornithinibacillus and Oceanobacillus.
31184567	1	55	theme	emended	107:113	arg1	description					115:125	emended description	107:125	emended description of the genus Ornithinibacillus	107:156	nov., a halophilic bacterium isolated from lake sediment, and emended description of the genus Ornithinibacillus.
31184567	14	56	theme	type	1456:1459	arg1	LJ137T					1471:1476	LJ137T	1471:1476	LJ137T (=CGMCC 1.13678T=NBRC 113552T)	1471:1507	The type strain is LJ137T (=CGMCC 1.13678T=NBRC 113552T).
31184567	14	56	theme	type	1456:1459	arg1	strain					1461:1466	The type strain	1452:1466	The type strain	1452:1466	The type strain is LJ137T (=CGMCC 1.13678T=NBRC 113552T).
31184567	12	57	theme	novel	1388:1392	arg1	species					1394:1400	a novel species	1386:1400	a novel species	1386:1400	Based on the phenotypic, chemotaxonomic, phylogenetic and genome sequence characteristics of this strain, a novel species, Ornithinibacillus gellani sp.
31184567	12	57	theme	novel	1388:1392	arg1	strain					1378:1383	this strain	1373:1383	this strain	1373:1383	Based on the phenotypic, chemotaxonomic, phylogenetic and genome sequence characteristics of this strain, a novel species, Ornithinibacillus gellani sp.
31184567	2	58	theme	motile	200:205	arg1	bacterium					222:230	A Gram-stain-positive, strictly aerobic, motile and rod-shaped bacterium	159:230	A Gram-stain-positive, strictly aerobic, motile and rod-shaped bacterium	159:230	A Gram-stain-positive, strictly aerobic, motile and rod-shaped bacterium, designated strain LJ137T, was isolated from the sediment of Taihu Lake in China.
31184567	6	59	theme	16S	804:806	arg1	similarity					827:836	95.2, 96.5 and 95.6 % 16S rRNA gene sequence similarity	782:836	95.2, 96.5 and 95.6 % 16S rRNA gene sequence similarity	782:836	The closest phylogenetic neighbours were Ornithinibacillus halophilus KCTC 13822T, Ornithinibacillus salinisoli LCB256T and Oceanobacillus limi KCTC 13823T, with 95.2, 96.5 and 95.6 % 16S rRNA gene sequence similarity, respectively.
31184567	6	60	theme	KCTC	764:767	arg1	13823T					769:774	KCTC 13823T	764:774	KCTC 13823T	764:774	The closest phylogenetic neighbours were Ornithinibacillus halophilus KCTC 13822T, Ornithinibacillus salinisoli LCB256T and Oceanobacillus limi KCTC 13823T, with 95.2, 96.5 and 95.6 % 16S rRNA gene sequence similarity, respectively.
31184567	5	61	theme	Phylogenetic	460:471	arg1	analysis					473:480	Phylogenetic analysis	460:480	Phylogenetic analysis based on 16S rRNA gene sequences	460:513	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain LJ137T was most closely related to the genera Ornithinibacillus and Oceanobacillus.
31184567	2	62	from	sediment	281:288	arg1	China					307:311	China	307:311	China	307:311	A Gram-stain-positive, strictly aerobic, motile and rod-shaped bacterium, designated strain LJ137T, was isolated from the sediment of Taihu Lake in China.
31184567	10	63	theme	DNA	1172:1174	arg1	%					1188:1188	40.4 mol%	1180:1188	40.4 mol%	1180:1188	The G+C content of the genomic DNA was 40.4 mol%.
31184567	10	63	theme	DNA	1172:1174	arg1	content					1149:1155	The G+C content	1141:1155	The G+C content of the genomic DNA	1141:1174	The G+C content of the genomic DNA was 40.4 mol%.
31184567	9	64	contain	contained	1000:1008	arg1	lipids					976:981	The polar lipids	966:981	The polar lipids of strain LJ137T	966:998	The polar lipids of strain LJ137T contained diphosphatidylglycerol, phosphatidylglycerol, three unidentified phospholipids, two aminophospholipids and one unidentified lipid.
31184567	9	64	contain	contained	1000:1008	arg2	phospholipids					1075:1087	three unidentified phospholipids	1056:1087	three unidentified phospholipids	1056:1087	The polar lipids of strain LJ137T contained diphosphatidylglycerol, phosphatidylglycerol, three unidentified phospholipids, two aminophospholipids and one unidentified lipid.
31184567	9	64	contain	contained	1000:1008	arg2	phosphatidylglycerol					1034:1053	phosphatidylglycerol	1034:1053	phosphatidylglycerol	1034:1053	The polar lipids of strain LJ137T contained diphosphatidylglycerol, phosphatidylglycerol, three unidentified phospholipids, two aminophospholipids and one unidentified lipid.
31184567	9	64	contain	contained	1000:1008	arg2	diphosphatidylglycerol					1010:1031	diphosphatidylglycerol	1010:1031	diphosphatidylglycerol	1010:1031	The polar lipids of strain LJ137T contained diphosphatidylglycerol, phosphatidylglycerol, three unidentified phospholipids, two aminophospholipids and one unidentified lipid.
31184567	9	64	contain	contained	1000:1008	arg2	lipid					1134:1138	one unidentified lipid	1117:1138	one unidentified lipid	1117:1138	The polar lipids of strain LJ137T contained diphosphatidylglycerol, phosphatidylglycerol, three unidentified phospholipids, two aminophospholipids and one unidentified lipid.
31184567	9	64	contain	contained	1000:1008	arg2	aminophospholipids					1094:1111	two aminophospholipids	1090:1111	two aminophospholipids	1090:1111	The polar lipids of strain LJ137T contained diphosphatidylglycerol, phosphatidylglycerol, three unidentified phospholipids, two aminophospholipids and one unidentified lipid.
31184567	12	65	theme	phylogenetic	1321:1332	arg1	characteristics					1354:1368	the phenotypic, chemotaxonomic, phylogenetic and genome sequence characteristics	1289:1368	the phenotypic, chemotaxonomic, phylogenetic and genome sequence characteristics of this strain, a novel species, Ornithinibacillus gellani sp	1289:1430	Based on the phenotypic, chemotaxonomic, phylogenetic and genome sequence characteristics of this strain, a novel species, Ornithinibacillus gellani sp.
31184567	9	66	theme	LJ137T	993:998	arg1	lipids					976:981	The polar lipids	966:981	The polar lipids of strain LJ137T	966:998	The polar lipids of strain LJ137T contained diphosphatidylglycerol, phosphatidylglycerol, three unidentified phospholipids, two aminophospholipids and one unidentified lipid.
31184567	10	67	theme	genomic	1164:1170	arg1	DNA					1172:1174	the genomic DNA	1160:1174	the genomic DNA	1160:1174	The G+C content of the genomic DNA was 40.4 mol%.
31184567	3	68	theme	polyphasic	316:325	arg1	approach					327:334	A polyphasic approach	314:334	A polyphasic approach	314:334	A polyphasic approach was used to investigate its taxonomic position.
31184567	12	69	theme	Ornithinibacillus	1403:1419	arg1	strain					1378:1383	this strain	1373:1383	this strain	1373:1383	Based on the phenotypic, chemotaxonomic, phylogenetic and genome sequence characteristics of this strain, a novel species, Ornithinibacillus gellani sp.
31184567	12	69	theme	Ornithinibacillus	1403:1419	arg1	sp					1429:1430	Ornithinibacillus gellani sp	1403:1430	Ornithinibacillus gellani sp	1403:1430	Based on the phenotypic, chemotaxonomic, phylogenetic and genome sequence characteristics of this strain, a novel species, Ornithinibacillus gellani sp.
31184567	2	70	theme	strain	244:249	arg1	LJ137T					251:256	strain LJ137T	244:256	strain LJ137T	244:256	A Gram-stain-positive, strictly aerobic, motile and rod-shaped bacterium, designated strain LJ137T, was isolated from the sediment of Taihu Lake in China.
31184567	1	71	theme	genus	134:138	arg1	Ornithinibacillus					140:156	the genus Ornithinibacillus	130:156	the genus Ornithinibacillus	130:156	nov., a halophilic bacterium isolated from lake sediment, and emended description of the genus Ornithinibacillus.
31184567	7	72	theme	peptidoglycan	857:869	arg1	type					882:885	The peptidoglycan amino acid type	853:885	The peptidoglycan amino acid type	853:885	The peptidoglycan amino acid type was A4α (l-Lys-d-Asp).
31184567	7	72	theme	peptidoglycan	857:869	arg1	A4α					891:893	A4α	891:893	A4α (l-Lys-d-Asp)	891:907	The peptidoglycan amino acid type was A4α (l-Lys-d-Asp).
31184567	11	73	dep	anteiso-C15 	1230:1241	arg1	 0					1260:1261	 0	1260:1261	 0	1260:1261	The dominant cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C15 : 0.
31184567	11	73	dep	anteiso-C15 	1230:1241	arg1	iso-C15 					1267:1274	iso-C15 	1267:1274	iso-C15 	1267:1274	The dominant cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C15 : 0.
31184567	11	73	dep	anteiso-C15 	1230:1241	arg1	 0					1243:1244	 0	1243:1244	 0	1243:1244	The dominant cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C15 : 0.
31184567	11	73	dep	anteiso-C15 	1230:1241	arg1	 0					1276:1277	 0	1276:1277	 0	1276:1277	The dominant cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C15 : 0.
30067175	3	0	theme	endospore-forming	220:236	arg1	bacteria					330:337	Gram-stain-positive, endospore-forming, motile by means of peritrichous flagella, aerobic or facultative anaerobic, and rod-shaped bacteria	199:337	Gram-stain-positive, endospore-forming, motile by means of peritrichous flagella, aerobic or facultative anaerobic, and rod-shaped bacteria	199:337	Two strains of Gram-stain-positive, endospore-forming, motile by means of peritrichous flagella, aerobic or facultative anaerobic, and rod-shaped bacteria that were designated ON8T and ON6T were isolated from soil collected from a mountain wetland in Gwang-ju, Republic of Korea.
30067175	9	1	theme	Paenibacillus	1220:1232	arg1	members					1199:1205	the members	1195:1205	the members of the genus Paenibacillus in the family Paenibacillaceae	1195:1263	The DNA G+C contents of strains ON8T and ON6T were 50.6 and 53.5 mol%, respectively, and the 16S rRNA gene sequence analysis showed that the nearest phylogenetic neighbour of both strains was Gorillibacterium massiliense G5T (93.9 %), followed by the members of the genus Paenibacillus in the family Paenibacillaceae.
30067175	9	2	theme	rRNA	1045:1048	arg1	analysis					1064:1071	the 16S rRNA gene sequence analysis	1037:1071	the 16S rRNA gene sequence analysis	1037:1071	The DNA G+C contents of strains ON8T and ON6T were 50.6 and 53.5 mol%, respectively, and the 16S rRNA gene sequence analysis showed that the nearest phylogenetic neighbour of both strains was Gorillibacterium massiliense G5T (93.9 %), followed by the members of the genus Paenibacillus in the family Paenibacillaceae.
30067175	3	3	theme	rod-shaped	319:328	arg1	bacteria					330:337	Gram-stain-positive, endospore-forming, motile by means of peritrichous flagella, aerobic or facultative anaerobic, and rod-shaped bacteria	199:337	Gram-stain-positive, endospore-forming, motile by means of peritrichous flagella, aerobic or facultative anaerobic, and rod-shaped bacteria	199:337	Two strains of Gram-stain-positive, endospore-forming, motile by means of peritrichous flagella, aerobic or facultative anaerobic, and rod-shaped bacteria that were designated ON8T and ON6T were isolated from soil collected from a mountain wetland in Gwang-ju, Republic of Korea.
30067175	13	4	dep	nov	1662:1664	arg1	31957T					1701:1706	type strain ON6T=KACC 19266T=JCM 31957T	1668:1706	type strain ON6T=KACC 19266T=JCM 31957T	1668:1706	nov. (type strain ON6T=KACC 19266T=JCM 31957T).
30067175	12	5	theme	strain	1597:1602	arg1	31958T					1625:1630	type strain ON8T=KACC 19267T=JCM 31958T	1592:1630	the type species; type strain ON8T=KACC 19267T=JCM 31958T	1574:1630	nov. (the type species; type strain ON8T=KACC 19267T=JCM 31958T) and Paludirhabdus pumila sp.
30067175	11	6	theme	polyphasic	1409:1418	arg1	characteristics					1420:1434	polyphasic characteristics	1409:1434	polyphasic characteristics	1409:1434	Based on polyphasic characteristics, a novel genus is proposed with the name Paludirhabdus gen. nov., which consists of two species, Paludirhabdus telluriireducens sp.
30067175	9	7	theme	Gorillibacterium	1140:1155	arg1	G5T					1169:1171	Gorillibacterium massiliense G5T	1140:1171	Gorillibacterium massiliense G5T (93.9 %)	1140:1180	The DNA G+C contents of strains ON8T and ON6T were 50.6 and 53.5 mol%, respectively, and the 16S rRNA gene sequence analysis showed that the nearest phylogenetic neighbour of both strains was Gorillibacterium massiliense G5T (93.9 %), followed by the members of the genus Paenibacillus in the family Paenibacillaceae.
30067175	9	7	theme	Gorillibacterium	1140:1155	arg1	%					1179:1179	93.9 %	1174:1179	93.9 %	1174:1179	The DNA G+C contents of strains ON8T and ON6T were 50.6 and 53.5 mol%, respectively, and the 16S rRNA gene sequence analysis showed that the nearest phylogenetic neighbour of both strains was Gorillibacterium massiliense G5T (93.9 %), followed by the members of the genus Paenibacillus in the family Paenibacillaceae.
30067175	9	7	theme	Gorillibacterium	1140:1155	arg1	neighbour					1110:1118	the nearest phylogenetic neighbour	1085:1118	the nearest phylogenetic neighbour of both strains	1085:1134	The DNA G+C contents of strains ON8T and ON6T were 50.6 and 53.5 mol%, respectively, and the 16S rRNA gene sequence analysis showed that the nearest phylogenetic neighbour of both strains was Gorillibacterium massiliense G5T (93.9 %), followed by the members of the genus Paenibacillus in the family Paenibacillaceae.
30067175	8	8	theme	cell-wall	923:931	arg1	peptidoglycan					933:945	the cell-wall peptidoglycan	919:945	the cell-wall peptidoglycan	919:945	Meso-diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan.
30067175	3	9	theme	Gram-stain-positive	199:217	arg1	bacteria					330:337	Gram-stain-positive, endospore-forming, motile by means of peritrichous flagella, aerobic or facultative anaerobic, and rod-shaped bacteria	199:337	Gram-stain-positive, endospore-forming, motile by means of peritrichous flagella, aerobic or facultative anaerobic, and rod-shaped bacteria	199:337	Two strains of Gram-stain-positive, endospore-forming, motile by means of peritrichous flagella, aerobic or facultative anaerobic, and rod-shaped bacteria that were designated ON8T and ON6T were isolated from soil collected from a mountain wetland in Gwang-ju, Republic of Korea.
30067175	8	10	theme	diamino	903:909	arg1	acid					911:914	the diagnostic diamino acid	888:914	the diagnostic diamino acid in the cell-wall peptidoglycan	888:945	Meso-diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan.
30067175	8	10	theme	diamino	903:909	arg1	acid					879:882	Meso-diaminopimelic acid	859:882	Meso-diaminopimelic acid	859:882	Meso-diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan.
30067175	13	11	theme	19266T=JCM	1690:1699	arg1	31957T					1701:1706	type strain ON6T=KACC 19266T=JCM 31957T	1668:1706	type strain ON6T=KACC 19266T=JCM 31957T	1668:1706	nov. (type strain ON6T=KACC 19266T=JCM 31957T).
30067175	7	12	theme	predominant	769:779	arg1	diphosphatidylglycerol					799:820	diphosphatidylglycerol	799:820	diphosphatidylglycerol	799:820	The predominant polar lipids were diphosphatidylglycerol, aminophospholipid and phospholipid.
30067175	7	12	theme	predominant	769:779	arg1	lipids					787:792	The predominant polar lipids	765:792	The predominant polar lipids	765:792	The predominant polar lipids were diphosphatidylglycerol, aminophospholipid and phospholipid.
30067175	10	13	theme	hybridization	1278:1290	arg1	%					1341:1341	44.1 %	1336:1341	44.1 %	1336:1341	The DNA-DNA hybridization relatedness value between ON8T and ON6T was 44.1 %, which indicated that they represented distinct species.
30067175	10	13	theme	hybridization	1278:1290	arg1	value					1304:1308	The DNA-DNA hybridization relatedness value	1266:1308	The DNA-DNA hybridization relatedness value between ON8T and ON6T	1266:1330	The DNA-DNA hybridization relatedness value between ON8T and ON6T was 44.1 %, which indicated that they represented distinct species.
30067175	2	14	theme	emended	131:137	arg1	description					139:149	a mountain wetland and emended description	108:149	a mountain wetland and emended description of Gorillibacterium massiliense	108:181	nov., isolated from soil of a mountain wetland and emended description of Gorillibacterium massiliense.
30067175	9	15	theme	phylogenetic	1097:1108	arg1	G5T					1169:1171	Gorillibacterium massiliense G5T	1140:1171	Gorillibacterium massiliense G5T (93.9 %)	1140:1180	The DNA G+C contents of strains ON8T and ON6T were 50.6 and 53.5 mol%, respectively, and the 16S rRNA gene sequence analysis showed that the nearest phylogenetic neighbour of both strains was Gorillibacterium massiliense G5T (93.9 %), followed by the members of the genus Paenibacillus in the family Paenibacillaceae.
30067175	9	15	theme	phylogenetic	1097:1108	arg1	neighbour					1110:1118	the nearest phylogenetic neighbour	1085:1118	the nearest phylogenetic neighbour of both strains	1085:1134	The DNA G+C contents of strains ON8T and ON6T were 50.6 and 53.5 mol%, respectively, and the 16S rRNA gene sequence analysis showed that the nearest phylogenetic neighbour of both strains was Gorillibacterium massiliense G5T (93.9 %), followed by the members of the genus Paenibacillus in the family Paenibacillaceae.
30067175	1	16	theme	Paludirhabdus	55:67	arg1	pumila					69:74	nov. and Paludirhabdus pumila	46:74	nov. and Paludirhabdus pumila	46:74	nov. and Paludirhabdus pumila sp.
30067175	6	17	dep	 0	727:728	arg1	 0					749:750	 0	749:750	 0	749:750	The major menaquinone was MK-7 and the major cellular fatty acids (>10 % of the total) were anteiso-C15 : 0, iso-C15 : 0, C14 : 0 and C16 : 0.
30067175	6	17	dep	 0	727:728	arg1	C14 					744:747	C14 	744:747	C14 	744:747	The major menaquinone was MK-7 and the major cellular fatty acids (>10 % of the total) were anteiso-C15 : 0, iso-C15 : 0, C14 : 0 and C16 : 0.
30067175	6	17	dep	 0	727:728	arg1	 0					761:762	 0	761:762	 0	761:762	The major menaquinone was MK-7 and the major cellular fatty acids (>10 % of the total) were anteiso-C15 : 0, iso-C15 : 0, C14 : 0 and C16 : 0.
30067175	6	17	dep	 0	727:728	arg1	C16 					756:759	C16 	756:759	C16 	756:759	The major menaquinone was MK-7 and the major cellular fatty acids (>10 % of the total) were anteiso-C15 : 0, iso-C15 : 0, C14 : 0 and C16 : 0.
30067175	11	18	theme	gen.	1491:1494	arg1	sp					1564:1565	Paludirhabdus telluriireducens sp	1533:1565	Paludirhabdus telluriireducens sp	1533:1565	Based on polyphasic characteristics, a novel genus is proposed with the name Paludirhabdus gen. nov., which consists of two species, Paludirhabdus telluriireducens sp.
30067175	11	18	theme	gen.	1491:1494	arg1	nov.					1496:1499	the name Paludirhabdus gen. nov.	1468:1499	the name Paludirhabdus gen. nov.	1468:1499	Based on polyphasic characteristics, a novel genus is proposed with the name Paludirhabdus gen. nov., which consists of two species, Paludirhabdus telluriireducens sp.
30067175	10	19	theme	distinct	1382:1389	arg1	species					1391:1397	distinct species	1382:1397	distinct species	1382:1397	The DNA-DNA hybridization relatedness value between ON8T and ON6T was 44.1 %, which indicated that they represented distinct species.
30067175	5	20	theme	optimal	592:598	arg1	15-40 °C					582:589	15-40 °C	582:589	15-40 °C	582:589	Cells of ON8T and ON6T grew at 15-35 °C (optimal 30 °C) and 15-40 °C (optimal 30 °C), respectively.
30067175	5	20	theme	optimal	592:598	arg1	30 °C					600:604	optimal 30 °C	592:604	optimal 30 °C	592:604	Cells of ON8T and ON6T grew at 15-35 °C (optimal 30 °C) and 15-40 °C (optimal 30 °C), respectively.
30067175	8	21	theme	diagnostic	892:901	arg1	acid					911:914	the diagnostic diamino acid	888:914	the diagnostic diamino acid in the cell-wall peptidoglycan	888:945	Meso-diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan.
30067175	8	21	theme	diagnostic	892:901	arg1	acid					879:882	Meso-diaminopimelic acid	859:882	Meso-diaminopimelic acid	859:882	Meso-diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan.
30067175	6	22	theme	fatty	676:680	arg1	anteiso-C15 					714:725	anteiso-C15 	714:725	anteiso-C15 	714:725	The major menaquinone was MK-7 and the major cellular fatty acids (>10 % of the total) were anteiso-C15 : 0, iso-C15 : 0, C14 : 0 and C16 : 0.
30067175	6	22	theme	fatty	676:680	arg1	acids					682:686	the major cellular fatty acids	657:686	the major cellular fatty acids (>10 % of the total)	657:707	The major menaquinone was MK-7 and the major cellular fatty acids (>10 % of the total) were anteiso-C15 : 0, iso-C15 : 0, C14 : 0 and C16 : 0.
30067175	13	23	theme	strain	1673:1678	arg1	31957T					1701:1706	type strain ON6T=KACC 19266T=JCM 31957T	1668:1706	type strain ON6T=KACC 19266T=JCM 31957T	1668:1706	nov. (type strain ON6T=KACC 19266T=JCM 31957T).
30067175	2	24	theme	wetland	119:125	arg1	description					139:149	a mountain wetland and emended description	108:149	a mountain wetland and emended description of Gorillibacterium massiliense	108:181	nov., isolated from soil of a mountain wetland and emended description of Gorillibacterium massiliense.
30067175	9	25	from	Paenibacillaceae	1248:1263	arg1	Paenibacillus					1220:1232	Paenibacillus	1220:1232	Paenibacillus	1220:1232	The DNA G+C contents of strains ON8T and ON6T were 50.6 and 53.5 mol%, respectively, and the 16S rRNA gene sequence analysis showed that the nearest phylogenetic neighbour of both strains was Gorillibacterium massiliense G5T (93.9 %), followed by the members of the genus Paenibacillus in the family Paenibacillaceae.
30067175	11	26	theme	name	1472:1475	arg1	sp					1564:1565	Paludirhabdus telluriireducens sp	1533:1565	Paludirhabdus telluriireducens sp	1533:1565	Based on polyphasic characteristics, a novel genus is proposed with the name Paludirhabdus gen. nov., which consists of two species, Paludirhabdus telluriireducens sp.
30067175	11	26	theme	name	1472:1475	arg1	nov.					1496:1499	the name Paludirhabdus gen. nov.	1468:1499	the name Paludirhabdus gen. nov.	1468:1499	Based on polyphasic characteristics, a novel genus is proposed with the name Paludirhabdus gen. nov., which consists of two species, Paludirhabdus telluriireducens sp.
30067175	6	27	theme	cellular	667:674	arg1	anteiso-C15 					714:725	anteiso-C15 	714:725	anteiso-C15 	714:725	The major menaquinone was MK-7 and the major cellular fatty acids (>10 % of the total) were anteiso-C15 : 0, iso-C15 : 0, C14 : 0 and C16 : 0.
30067175	6	27	theme	cellular	667:674	arg1	acids					682:686	the major cellular fatty acids	657:686	the major cellular fatty acids (>10 % of the total)	657:707	The major menaquinone was MK-7 and the major cellular fatty acids (>10 % of the total) were anteiso-C15 : 0, iso-C15 : 0, C14 : 0 and C16 : 0.
30067175	13	28	theme	type	1668:1671	arg1	31957T					1701:1706	type strain ON6T=KACC 19266T=JCM 31957T	1668:1706	type strain ON6T=KACC 19266T=JCM 31957T	1668:1706	nov. (type strain ON6T=KACC 19266T=JCM 31957T).
30067175	2	29	theme	mountain	110:117	arg1	description					139:149	a mountain wetland and emended description	108:149	a mountain wetland and emended description of Gorillibacterium massiliense	108:181	nov., isolated from soil of a mountain wetland and emended description of Gorillibacterium massiliense.
30067175	3	30	theme	motile	239:244	arg1	bacteria					330:337	Gram-stain-positive, endospore-forming, motile by means of peritrichous flagella, aerobic or facultative anaerobic, and rod-shaped bacteria	199:337	Gram-stain-positive, endospore-forming, motile by means of peritrichous flagella, aerobic or facultative anaerobic, and rod-shaped bacteria	199:337	Two strains of Gram-stain-positive, endospore-forming, motile by means of peritrichous flagella, aerobic or facultative anaerobic, and rod-shaped bacteria that were designated ON8T and ON6T were isolated from soil collected from a mountain wetland in Gwang-ju, Republic of Korea.
30067175	5	31	theme	optimal	563:569	arg1	30 °C					571:575	optimal 30 °C	563:575	optimal 30 °C	563:575	Cells of ON8T and ON6T grew at 15-35 °C (optimal 30 °C) and 15-40 °C (optimal 30 °C), respectively.
30067175	5	31	theme	optimal	563:569	arg1	15-35 °C					553:560	15-35 °C	553:560	15-35 °C	553:560	Cells of ON8T and ON6T grew at 15-35 °C (optimal 30 °C) and 15-40 °C (optimal 30 °C), respectively.
30067175	6	32	theme	major	661:665	arg1	anteiso-C15 					714:725	anteiso-C15 	714:725	anteiso-C15 	714:725	The major menaquinone was MK-7 and the major cellular fatty acids (>10 % of the total) were anteiso-C15 : 0, iso-C15 : 0, C14 : 0 and C16 : 0.
30067175	6	32	theme	major	661:665	arg1	acids					682:686	the major cellular fatty acids	657:686	the major cellular fatty acids (>10 % of the total)	657:707	The major menaquinone was MK-7 and the major cellular fatty acids (>10 % of the total) were anteiso-C15 : 0, iso-C15 : 0, C14 : 0 and C16 : 0.
30067175	3	33	theme	mountain	415:422	arg1	wetland					424:430	a mountain wetland	413:430	a mountain wetland in Gwang-ju, Republic of Korea	413:461	Two strains of Gram-stain-positive, endospore-forming, motile by means of peritrichous flagella, aerobic or facultative anaerobic, and rod-shaped bacteria that were designated ON8T and ON6T were isolated from soil collected from a mountain wetland in Gwang-ju, Republic of Korea.
30067175	9	34	from	Paenibacillus	1220:1232	arg1	Paenibacillaceae					1248:1263	the family Paenibacillaceae	1237:1263	the family Paenibacillaceae	1237:1263	The DNA G+C contents of strains ON8T and ON6T were 50.6 and 53.5 mol%, respectively, and the 16S rRNA gene sequence analysis showed that the nearest phylogenetic neighbour of both strains was Gorillibacterium massiliense G5T (93.9 %), followed by the members of the genus Paenibacillus in the family Paenibacillaceae.
30067175	6	35	dep	acids	682:686	arg1	%					693:693	>10 %	689:693	>10 %	689:693	The major menaquinone was MK-7 and the major cellular fatty acids (>10 % of the total) were anteiso-C15 : 0, iso-C15 : 0, C14 : 0 and C16 : 0.
30067175	6	35	dep	acids	682:686	arg1	total					702:706	total	702:706	total	702:706	The major menaquinone was MK-7 and the major cellular fatty acids (>10 % of the total) were anteiso-C15 : 0, iso-C15 : 0, C14 : 0 and C16 : 0.
30067175	11	36	theme	Paludirhabdus	1477:1489	arg1	sp					1564:1565	Paludirhabdus telluriireducens sp	1533:1565	Paludirhabdus telluriireducens sp	1533:1565	Based on polyphasic characteristics, a novel genus is proposed with the name Paludirhabdus gen. nov., which consists of two species, Paludirhabdus telluriireducens sp.
30067175	11	36	theme	Paludirhabdus	1477:1489	arg1	nov.					1496:1499	the name Paludirhabdus gen. nov.	1468:1499	the name Paludirhabdus gen. nov.	1468:1499	Based on polyphasic characteristics, a novel genus is proposed with the name Paludirhabdus gen. nov., which consists of two species, Paludirhabdus telluriireducens sp.
30067175	9	37	theme	massiliense	1157:1167	arg1	G5T					1169:1171	Gorillibacterium massiliense G5T	1140:1171	Gorillibacterium massiliense G5T (93.9 %)	1140:1180	The DNA G+C contents of strains ON8T and ON6T were 50.6 and 53.5 mol%, respectively, and the 16S rRNA gene sequence analysis showed that the nearest phylogenetic neighbour of both strains was Gorillibacterium massiliense G5T (93.9 %), followed by the members of the genus Paenibacillus in the family Paenibacillaceae.
30067175	9	37	theme	massiliense	1157:1167	arg1	%					1179:1179	93.9 %	1174:1179	93.9 %	1174:1179	The DNA G+C contents of strains ON8T and ON6T were 50.6 and 53.5 mol%, respectively, and the 16S rRNA gene sequence analysis showed that the nearest phylogenetic neighbour of both strains was Gorillibacterium massiliense G5T (93.9 %), followed by the members of the genus Paenibacillus in the family Paenibacillaceae.
30067175	9	37	theme	massiliense	1157:1167	arg1	neighbour					1110:1118	the nearest phylogenetic neighbour	1085:1118	the nearest phylogenetic neighbour of both strains	1085:1134	The DNA G+C contents of strains ON8T and ON6T were 50.6 and 53.5 mol%, respectively, and the 16S rRNA gene sequence analysis showed that the nearest phylogenetic neighbour of both strains was Gorillibacterium massiliense G5T (93.9 %), followed by the members of the genus Paenibacillus in the family Paenibacillaceae.
30067175	9	38	theme	strains	1128:1134	arg1	G5T					1169:1171	Gorillibacterium massiliense G5T	1140:1171	Gorillibacterium massiliense G5T (93.9 %)	1140:1180	The DNA G+C contents of strains ON8T and ON6T were 50.6 and 53.5 mol%, respectively, and the 16S rRNA gene sequence analysis showed that the nearest phylogenetic neighbour of both strains was Gorillibacterium massiliense G5T (93.9 %), followed by the members of the genus Paenibacillus in the family Paenibacillaceae.
30067175	9	38	theme	strains	1128:1134	arg1	neighbour					1110:1118	the nearest phylogenetic neighbour	1085:1118	the nearest phylogenetic neighbour of both strains	1085:1134	The DNA G+C contents of strains ON8T and ON6T were 50.6 and 53.5 mol%, respectively, and the 16S rRNA gene sequence analysis showed that the nearest phylogenetic neighbour of both strains was Gorillibacterium massiliense G5T (93.9 %), followed by the members of the genus Paenibacillus in the family Paenibacillaceae.
30067175	10	39	theme	relatedness	1292:1302	arg1	%					1341:1341	44.1 %	1336:1341	44.1 %	1336:1341	The DNA-DNA hybridization relatedness value between ON8T and ON6T was 44.1 %, which indicated that they represented distinct species.
30067175	10	39	theme	relatedness	1292:1302	arg1	value					1304:1308	The DNA-DNA hybridization relatedness value	1266:1308	The DNA-DNA hybridization relatedness value between ON8T and ON6T	1266:1330	The DNA-DNA hybridization relatedness value between ON8T and ON6T was 44.1 %, which indicated that they represented distinct species.
30067175	9	40	theme	nearest	1089:1095	arg1	G5T					1169:1171	Gorillibacterium massiliense G5T	1140:1171	Gorillibacterium massiliense G5T (93.9 %)	1140:1180	The DNA G+C contents of strains ON8T and ON6T were 50.6 and 53.5 mol%, respectively, and the 16S rRNA gene sequence analysis showed that the nearest phylogenetic neighbour of both strains was Gorillibacterium massiliense G5T (93.9 %), followed by the members of the genus Paenibacillus in the family Paenibacillaceae.
30067175	9	40	theme	nearest	1089:1095	arg1	neighbour					1110:1118	the nearest phylogenetic neighbour	1085:1118	the nearest phylogenetic neighbour of both strains	1085:1134	The DNA G+C contents of strains ON8T and ON6T were 50.6 and 53.5 mol%, respectively, and the 16S rRNA gene sequence analysis showed that the nearest phylogenetic neighbour of both strains was Gorillibacterium massiliense G5T (93.9 %), followed by the members of the genus Paenibacillus in the family Paenibacillaceae.
30067175	0	41	theme	gen.	31:34	arg1	sp					42:43	sp	42:43	sp	42:43	Paludirhabdus telluriireducens gen. nov., sp.
30067175	0	41	theme	gen.	31:34	arg1	nov.					36:39	gen. nov.	31:39	gen. nov.	31:39	Paludirhabdus telluriireducens gen. nov., sp.
30067175	9	42	theme	family	1241:1246	arg1	Paenibacillaceae					1248:1263	the family Paenibacillaceae	1237:1263	the family Paenibacillaceae	1237:1263	The DNA G+C contents of strains ON8T and ON6T were 50.6 and 53.5 mol%, respectively, and the 16S rRNA gene sequence analysis showed that the nearest phylogenetic neighbour of both strains was Gorillibacterium massiliense G5T (93.9 %), followed by the members of the genus Paenibacillus in the family Paenibacillaceae.
30067175	3	43	theme	aerobic	281:287	arg1	bacteria					330:337	Gram-stain-positive, endospore-forming, motile by means of peritrichous flagella, aerobic or facultative anaerobic, and rod-shaped bacteria	199:337	Gram-stain-positive, endospore-forming, motile by means of peritrichous flagella, aerobic or facultative anaerobic, and rod-shaped bacteria	199:337	Two strains of Gram-stain-positive, endospore-forming, motile by means of peritrichous flagella, aerobic or facultative anaerobic, and rod-shaped bacteria that were designated ON8T and ON6T were isolated from soil collected from a mountain wetland in Gwang-ju, Republic of Korea.
30067175	2	44	theme	massiliense	171:181	arg1	description					139:149	a mountain wetland and emended description	108:149	a mountain wetland and emended description of Gorillibacterium massiliense	108:181	nov., isolated from soil of a mountain wetland and emended description of Gorillibacterium massiliense.
30067175	6	45	dep	anteiso-C15 	714:725	arg1	 0					727:728	 0	727:728	 0	727:728	The major menaquinone was MK-7 and the major cellular fatty acids (>10 % of the total) were anteiso-C15 : 0, iso-C15 : 0, C14 : 0 and C16 : 0.
30067175	3	46	theme	peritrichous	258:269	arg1	flagella					271:278	peritrichous flagella	258:278	peritrichous flagella	258:278	Two strains of Gram-stain-positive, endospore-forming, motile by means of peritrichous flagella, aerobic or facultative anaerobic, and rod-shaped bacteria that were designated ON8T and ON6T were isolated from soil collected from a mountain wetland in Gwang-ju, Republic of Korea.
30067175	2	47	theme	Gorillibacterium	154:169	arg1	massiliense					171:181	Gorillibacterium massiliense	154:181	Gorillibacterium massiliense	154:181	nov., isolated from soil of a mountain wetland and emended description of Gorillibacterium massiliense.
30067175	12	48	theme	ON8T=KACC	1604:1612	arg1	31958T					1625:1630	type strain ON8T=KACC 19267T=JCM 31958T	1592:1630	the type species; type strain ON8T=KACC 19267T=JCM 31958T	1574:1630	nov. (the type species; type strain ON8T=KACC 19267T=JCM 31958T) and Paludirhabdus pumila sp.
30067175	12	49	theme	type	1578:1581	arg1	species					1583:1589	the type species	1574:1589	the type species; type strain ON8T=KACC 19267T=JCM 31958T	1574:1630	nov. (the type species; type strain ON8T=KACC 19267T=JCM 31958T) and Paludirhabdus pumila sp.
30067175	12	49	theme	type	1578:1581	arg1	nov					1568:1570	nov	1568:1570	nov	1568:1570	nov. (the type species; type strain ON8T=KACC 19267T=JCM 31958T) and Paludirhabdus pumila sp.
30067175	5	50	theme	ON6T	540:543	arg1	Cells					522:526	Cells	522:526	Cells of ON8T and ON6T	522:543	Cells of ON8T and ON6T grew at 15-35 °C (optimal 30 °C) and 15-40 °C (optimal 30 °C), respectively.
30067175	2	51	theme	description	139:149	arg1	soil					100:103	soil	100:103	soil of a mountain wetland and emended description of Gorillibacterium massiliense	100:181	nov., isolated from soil of a mountain wetland and emended description of Gorillibacterium massiliense.
30067175	3	52	dep	aerobic	281:287	arg1	anaerobic					304:312	anaerobic	304:312	anaerobic	304:312	Two strains of Gram-stain-positive, endospore-forming, motile by means of peritrichous flagella, aerobic or facultative anaerobic, and rod-shaped bacteria that were designated ON8T and ON6T were isolated from soil collected from a mountain wetland in Gwang-ju, Republic of Korea.
30067175	9	53	theme	16S	1041:1043	arg1	rRNA					1045:1048	the 16S rRNA	1037:1048	the 16S rRNA gene sequence analysis	1037:1071	The DNA G+C contents of strains ON8T and ON6T were 50.6 and 53.5 mol%, respectively, and the 16S rRNA gene sequence analysis showed that the nearest phylogenetic neighbour of both strains was Gorillibacterium massiliense G5T (93.9 %), followed by the members of the genus Paenibacillus in the family Paenibacillaceae.
30067175	11	54	theme	Paludirhabdus	1533:1545	arg1	sp					1564:1565	Paludirhabdus telluriireducens sp	1533:1565	Paludirhabdus telluriireducens sp	1533:1565	Based on polyphasic characteristics, a novel genus is proposed with the name Paludirhabdus gen. nov., which consists of two species, Paludirhabdus telluriireducens sp.
30067175	11	54	theme	Paludirhabdus	1533:1545	arg1	nov.					1496:1499	the name Paludirhabdus gen. nov.	1468:1499	the name Paludirhabdus gen. nov.	1468:1499	Based on polyphasic characteristics, a novel genus is proposed with the name Paludirhabdus gen. nov., which consists of two species, Paludirhabdus telluriireducens sp.
30067175	12	55	dep	species	1583:1589	arg1	31958T					1625:1630	type strain ON8T=KACC 19267T=JCM 31958T	1592:1630	the type species; type strain ON8T=KACC 19267T=JCM 31958T	1574:1630	nov. (the type species; type strain ON8T=KACC 19267T=JCM 31958T) and Paludirhabdus pumila sp.
30067175	6	56	theme	total	702:706	arg1	%					693:693	>10 %	689:693	>10 %	689:693	The major menaquinone was MK-7 and the major cellular fatty acids (>10 % of the total) were anteiso-C15 : 0, iso-C15 : 0, C14 : 0 and C16 : 0.
30067175	6	56	theme	total	702:706	arg1	total					702:706	total	702:706	total	702:706	The major menaquinone was MK-7 and the major cellular fatty acids (>10 % of the total) were anteiso-C15 : 0, iso-C15 : 0, C14 : 0 and C16 : 0.
30067175	1	57	dep	sp	76:77	arg1	pumila					69:74	nov. and Paludirhabdus pumila	46:74	nov. and Paludirhabdus pumila	46:74	nov. and Paludirhabdus pumila sp.
30067175	9	58	theme	DNA	952:954	arg1	50.6					999:1002	50.6	999:1002	50.6	999:1002	The DNA G+C contents of strains ON8T and ON6T were 50.6 and 53.5 mol%, respectively, and the 16S rRNA gene sequence analysis showed that the nearest phylogenetic neighbour of both strains was Gorillibacterium massiliense G5T (93.9 %), followed by the members of the genus Paenibacillus in the family Paenibacillaceae.
30067175	9	58	theme	DNA	952:954	arg1	G+C contents					956:967	The DNA G+C contents	948:967	The DNA G+C contents of strains ON8T and ON6T	948:992	The DNA G+C contents of strains ON8T and ON6T were 50.6 and 53.5 mol%, respectively, and the 16S rRNA gene sequence analysis showed that the nearest phylogenetic neighbour of both strains was Gorillibacterium massiliense G5T (93.9 %), followed by the members of the genus Paenibacillus in the family Paenibacillaceae.
30067175	9	59	theme	genus	1214:1218	arg1	members					1199:1205	the members	1195:1205	the members of the genus Paenibacillus in the family Paenibacillaceae	1195:1263	The DNA G+C contents of strains ON8T and ON6T were 50.6 and 53.5 mol%, respectively, and the 16S rRNA gene sequence analysis showed that the nearest phylogenetic neighbour of both strains was Gorillibacterium massiliense G5T (93.9 %), followed by the members of the genus Paenibacillus in the family Paenibacillaceae.
30067175	3	60	attach	isolated	379:386	arg2	strains					188:194	Two strains	184:194	Two strains of Gram-stain-positive, endospore-forming, motile by means of peritrichous flagella, aerobic or facultative anaerobic, and rod-shaped bacteria that were designated ON8T and ON6T	184:372	Two strains of Gram-stain-positive, endospore-forming, motile by means of peritrichous flagella, aerobic or facultative anaerobic, and rod-shaped bacteria that were designated ON8T and ON6T were isolated from soil collected from a mountain wetland in Gwang-ju, Republic of Korea.
30067175	3	60	attach	isolated	379:386	arg1	soil					393:396	soil	393:396	soil collected from a mountain wetland in Gwang-ju, Republic of Korea	393:461	Two strains of Gram-stain-positive, endospore-forming, motile by means of peritrichous flagella, aerobic or facultative anaerobic, and rod-shaped bacteria that were designated ON8T and ON6T were isolated from soil collected from a mountain wetland in Gwang-ju, Republic of Korea.
30067175	11	61	theme	novel	1439:1443	arg1	genus					1445:1449	a novel genus	1437:1449	a novel genus	1437:1449	Based on polyphasic characteristics, a novel genus is proposed with the name Paludirhabdus gen. nov., which consists of two species, Paludirhabdus telluriireducens sp.
30067175	9	62	theme	gene	1050:1053	arg1	analysis					1064:1071	the 16S rRNA gene sequence analysis	1037:1071	the 16S rRNA gene sequence analysis	1037:1071	The DNA G+C contents of strains ON8T and ON6T were 50.6 and 53.5 mol%, respectively, and the 16S rRNA gene sequence analysis showed that the nearest phylogenetic neighbour of both strains was Gorillibacterium massiliense G5T (93.9 %), followed by the members of the genus Paenibacillus in the family Paenibacillaceae.
30067175	8	63	from	acid	911:914	arg1	peptidoglycan					933:945	the cell-wall peptidoglycan	919:945	the cell-wall peptidoglycan	919:945	Meso-diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan.
30067175	6	64	theme	major	626:630	arg1	MK-7					648:651	MK-7	648:651	MK-7	648:651	The major menaquinone was MK-7 and the major cellular fatty acids (>10 % of the total) were anteiso-C15 : 0, iso-C15 : 0, C14 : 0 and C16 : 0.
30067175	6	64	theme	major	626:630	arg1	menaquinone					632:642	The major menaquinone	622:642	The major menaquinone	622:642	The major menaquinone was MK-7 and the major cellular fatty acids (>10 % of the total) were anteiso-C15 : 0, iso-C15 : 0, C14 : 0 and C16 : 0.
30067175	12	65	dep	sp	1658:1659	arg1	species					1583:1589	the type species	1574:1589	the type species; type strain ON8T=KACC 19267T=JCM 31958T	1574:1630	nov. (the type species; type strain ON8T=KACC 19267T=JCM 31958T) and Paludirhabdus pumila sp.
30067175	12	65	dep	sp	1658:1659	arg1	nov					1568:1570	nov	1568:1570	nov	1568:1570	nov. (the type species; type strain ON8T=KACC 19267T=JCM 31958T) and Paludirhabdus pumila sp.
30067175	12	65	dep	sp	1658:1659	arg1	pumila					1651:1656	pumila	1651:1656	pumila	1651:1656	nov. (the type species; type strain ON8T=KACC 19267T=JCM 31958T) and Paludirhabdus pumila sp.
30067175	9	66	dep	strains	972:978	arg1	strains					972:978	strains ON8T and ON6T	972:992	strains ON8T and ON6T	972:992	The DNA G+C contents of strains ON8T and ON6T were 50.6 and 53.5 mol%, respectively, and the 16S rRNA gene sequence analysis showed that the nearest phylogenetic neighbour of both strains was Gorillibacterium massiliense G5T (93.9 %), followed by the members of the genus Paenibacillus in the family Paenibacillaceae.
30067175	9	66	dep	strains	972:978	arg1	ON6T					989:992	ON6T	989:992	ON6T	989:992	The DNA G+C contents of strains ON8T and ON6T were 50.6 and 53.5 mol%, respectively, and the 16S rRNA gene sequence analysis showed that the nearest phylogenetic neighbour of both strains was Gorillibacterium massiliense G5T (93.9 %), followed by the members of the genus Paenibacillus in the family Paenibacillaceae.
30067175	9	66	dep	strains	972:978	arg1	ON8T					980:983	ON8T	980:983	ON8T	980:983	The DNA G+C contents of strains ON8T and ON6T were 50.6 and 53.5 mol%, respectively, and the 16S rRNA gene sequence analysis showed that the nearest phylogenetic neighbour of both strains was Gorillibacterium massiliense G5T (93.9 %), followed by the members of the genus Paenibacillus in the family Paenibacillaceae.
30067175	9	67	theme	sequence	1055:1062	arg1	analysis					1064:1071	the 16S rRNA gene sequence analysis	1037:1071	the 16S rRNA gene sequence analysis	1037:1071	The DNA G+C contents of strains ON8T and ON6T were 50.6 and 53.5 mol%, respectively, and the 16S rRNA gene sequence analysis showed that the nearest phylogenetic neighbour of both strains was Gorillibacterium massiliense G5T (93.9 %), followed by the members of the genus Paenibacillus in the family Paenibacillaceae.
30067175	12	68	theme	19267T=JCM	1614:1623	arg1	31958T					1625:1630	type strain ON8T=KACC 19267T=JCM 31958T	1592:1630	the type species; type strain ON8T=KACC 19267T=JCM 31958T	1574:1630	nov. (the type species; type strain ON8T=KACC 19267T=JCM 31958T) and Paludirhabdus pumila sp.
30067175	9	69	theme	53.5 mol	1008:1015	arg1	%					1016:1016	53.5 mol%	1008:1016	53.5 mol%	1008:1016	The DNA G+C contents of strains ON8T and ON6T were 50.6 and 53.5 mol%, respectively, and the 16S rRNA gene sequence analysis showed that the nearest phylogenetic neighbour of both strains was Gorillibacterium massiliense G5T (93.9 %), followed by the members of the genus Paenibacillus in the family Paenibacillaceae.
30067175	3	70	theme	bacteria	330:337	arg1	strains					188:194	Two strains	184:194	Two strains of Gram-stain-positive, endospore-forming, motile by means of peritrichous flagella, aerobic or facultative anaerobic, and rod-shaped bacteria that were designated ON8T and ON6T	184:372	Two strains of Gram-stain-positive, endospore-forming, motile by means of peritrichous flagella, aerobic or facultative anaerobic, and rod-shaped bacteria that were designated ON8T and ON6T were isolated from soil collected from a mountain wetland in Gwang-ju, Republic of Korea.
30067175	10	71	theme	DNA-DNA	1270:1276	arg1	%					1341:1341	44.1 %	1336:1341	44.1 %	1336:1341	The DNA-DNA hybridization relatedness value between ON8T and ON6T was 44.1 %, which indicated that they represented distinct species.
30067175	10	71	theme	DNA-DNA	1270:1276	arg1	value					1304:1308	The DNA-DNA hybridization relatedness value	1266:1308	The DNA-DNA hybridization relatedness value between ON8T and ON6T	1266:1330	The DNA-DNA hybridization relatedness value between ON8T and ON6T was 44.1 %, which indicated that they represented distinct species.
30067175	3	72	from	wetland	424:430	arg1	Republic					445:452	Republic	445:452	Republic	445:452	Two strains of Gram-stain-positive, endospore-forming, motile by means of peritrichous flagella, aerobic or facultative anaerobic, and rod-shaped bacteria that were designated ON8T and ON6T were isolated from soil collected from a mountain wetland in Gwang-ju, Republic of Korea.
30067175	9	73	theme	strains	972:978	arg1	50.6					999:1002	50.6	999:1002	50.6	999:1002	The DNA G+C contents of strains ON8T and ON6T were 50.6 and 53.5 mol%, respectively, and the 16S rRNA gene sequence analysis showed that the nearest phylogenetic neighbour of both strains was Gorillibacterium massiliense G5T (93.9 %), followed by the members of the genus Paenibacillus in the family Paenibacillaceae.
30067175	9	73	theme	strains	972:978	arg1	G+C contents					956:967	The DNA G+C contents	948:967	The DNA G+C contents of strains ON8T and ON6T	948:992	The DNA G+C contents of strains ON8T and ON6T were 50.6 and 53.5 mol%, respectively, and the 16S rRNA gene sequence analysis showed that the nearest phylogenetic neighbour of both strains was Gorillibacterium massiliense G5T (93.9 %), followed by the members of the genus Paenibacillus in the family Paenibacillaceae.
30067175	13	74	theme	ON6T=KACC	1680:1688	arg1	31957T					1701:1706	type strain ON6T=KACC 19266T=JCM 31957T	1668:1706	type strain ON6T=KACC 19266T=JCM 31957T	1668:1706	nov. (type strain ON6T=KACC 19266T=JCM 31957T).
30067175	11	75	theme	telluriireducens	1547:1562	arg1	sp					1564:1565	Paludirhabdus telluriireducens sp	1533:1565	Paludirhabdus telluriireducens sp	1533:1565	Based on polyphasic characteristics, a novel genus is proposed with the name Paludirhabdus gen. nov., which consists of two species, Paludirhabdus telluriireducens sp.
30067175	11	75	theme	telluriireducens	1547:1562	arg1	nov.					1496:1499	the name Paludirhabdus gen. nov.	1468:1499	the name Paludirhabdus gen. nov.	1468:1499	Based on polyphasic characteristics, a novel genus is proposed with the name Paludirhabdus gen. nov., which consists of two species, Paludirhabdus telluriireducens sp.
30067175	1	76	theme	nov.	46:49	arg1	pumila					69:74	nov. and Paludirhabdus pumila	46:74	nov. and Paludirhabdus pumila	46:74	nov. and Paludirhabdus pumila sp.
30067175	5	77	theme	ON8T	531:534	arg1	Cells					522:526	Cells	522:526	Cells of ON8T and ON6T	522:543	Cells of ON8T and ON6T grew at 15-35 °C (optimal 30 °C) and 15-40 °C (optimal 30 °C), respectively.
30067175	8	78	theme	Meso-diaminopimelic	859:877	arg1	acid					911:914	the diagnostic diamino acid	888:914	the diagnostic diamino acid in the cell-wall peptidoglycan	888:945	Meso-diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan.
30067175	8	78	theme	Meso-diaminopimelic	859:877	arg1	acid					879:882	Meso-diaminopimelic acid	859:882	Meso-diaminopimelic acid	859:882	Meso-diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan.
30067175	7	79	theme	polar	781:785	arg1	diphosphatidylglycerol					799:820	diphosphatidylglycerol	799:820	diphosphatidylglycerol	799:820	The predominant polar lipids were diphosphatidylglycerol, aminophospholipid and phospholipid.
30067175	7	79	theme	polar	781:785	arg1	lipids					787:792	The predominant polar lipids	765:792	The predominant polar lipids	765:792	The predominant polar lipids were diphosphatidylglycerol, aminophospholipid and phospholipid.
30067175	12	80	theme	type	1592:1595	arg1	31958T					1625:1630	type strain ON8T=KACC 19267T=JCM 31958T	1592:1630	the type species; type strain ON8T=KACC 19267T=JCM 31958T	1574:1630	nov. (the type species; type strain ON8T=KACC 19267T=JCM 31958T) and Paludirhabdus pumila sp.
30010734	6	0	theme	gene	1107:1110	arg1	sequencing					1112:1121	16S rRNA gene sequencing	1098:1121	16S rRNA gene sequencing	1098:1121	Analysis of the gut microbiome and metabolome was carried out by 16S rRNA gene sequencing and multiple mass spectrometry platforms.
30010734	10	1	theme	tryptophan	2033:2042	arg1	metabolism					2044:2053	tryptophan metabolism	2033:2053	tryptophan metabolism of gut microbiota in the recipient colon, which may play a potential role in maintenance of the intestinal barrier	2033:2168	CONCLUSIONS Our data reveal a regulatory effect of FMT on tryptophan metabolism of gut microbiota in the recipient colon, which may play a potential role in maintenance of the intestinal barrier.
30010734	2	2	theme	exogenous	347:355	arg1	microbiota					364:373	exogenous faecal microbiota	347:373	exogenous faecal microbiota	347:373	Using a piglet model, our previous study indicated that exogenous faecal microbiota can increase the expressions of tight junction proteins, mucin and antimicrobial peptide in the intestinal mucosa, suggesting a beneficial effect of FMT on gut barrier and gastrointestinal health.
30010734	1	3	theme	bowel	270:274	arg1	disease					276:282	inflammatory bowel disease	257:282	inflammatory bowel disease [IBD]	257:288	BACKGROUND AND AIMS Faecal microbiota transplantation [FMT] has shown promise as a treatment for inflammatory bowel disease [IBD].
30010734	10	4	from	effect	2016:2021	arg1	metabolism					2044:2053	tryptophan metabolism	2033:2053	tryptophan metabolism of gut microbiota in the recipient colon, which may play a potential role in maintenance of the intestinal barrier	2033:2168	CONCLUSIONS Our data reveal a regulatory effect of FMT on tryptophan metabolism of gut microbiota in the recipient colon, which may play a potential role in maintenance of the intestinal barrier.
30010734	9	5	theme	prediction	1613:1622	arg1	analysis					1624:1631	metagenomics prediction analysis	1600:1631	metagenomics prediction analysis based on 16S rRNA gene sequencing	1600:1665	In concordance with the metabolome data, metagenomics prediction analysis based on 16S rRNA gene sequencing also demonstrated that FMT modulated the metabolic functions of gut microbiota associated with indole alkaloid biosynthesis, cytochrome P450 and intestinal homeostasis, which coincided with up-regulation of cytokine interleukin-22 and enhanced activation of aryl hydrocarbon receptor in the recipient colon.
30010734	9	6	theme	indole	1762:1767	arg1	biosynthesis					1778:1789	indole alkaloid biosynthesis	1762:1789	indole alkaloid biosynthesis	1762:1789	In concordance with the metabolome data, metagenomics prediction analysis based on 16S rRNA gene sequencing also demonstrated that FMT modulated the metabolic functions of gut microbiota associated with indole alkaloid biosynthesis, cytochrome P450 and intestinal homeostasis, which coincided with up-regulation of cytokine interleukin-22 and enhanced activation of aryl hydrocarbon receptor in the recipient colon.
30010734	7	7	theme	LPS-induced	1273:1283	arg1	destruction					1285:1295	LPS-induced destruction	1273:1295	LPS-induced destruction of epithelial integrity and severe inflammatory response	1273:1352	RESULTS FMT modulated the diversity and composition of colonic microbiota and reduced the susceptibility to LPS-induced destruction of epithelial integrity and severe inflammatory response.
30010734	10	8	theme	microbiota	2062:2071	arg1	metabolism					2044:2053	tryptophan metabolism	2033:2053	tryptophan metabolism of gut microbiota in the recipient colon, which may play a potential role in maintenance of the intestinal barrier	2033:2168	CONCLUSIONS Our data reveal a regulatory effect of FMT on tryptophan metabolism of gut microbiota in the recipient colon, which may play a potential role in maintenance of the intestinal barrier.
30010734	9	9	theme	receptor	1942:1949	arg1	activation					1911:1920	enhanced activation	1902:1920	enhanced activation of aryl hydrocarbon receptor in the recipient colon	1902:1972	In concordance with the metabolome data, metagenomics prediction analysis based on 16S rRNA gene sequencing also demonstrated that FMT modulated the metabolic functions of gut microbiota associated with indole alkaloid biosynthesis, cytochrome P450 and intestinal homeostasis, which coincided with up-regulation of cytokine interleukin-22 and enhanced activation of aryl hydrocarbon receptor in the recipient colon.
30010734	9	9	theme	receptor	1942:1949	arg1	up-regulation					1857:1869	up-regulation	1857:1869	up-regulation of cytokine interleukin-22	1857:1896	In concordance with the metabolome data, metagenomics prediction analysis based on 16S rRNA gene sequencing also demonstrated that FMT modulated the metabolic functions of gut microbiota associated with indole alkaloid biosynthesis, cytochrome P450 and intestinal homeostasis, which coincided with up-regulation of cytokine interleukin-22 and enhanced activation of aryl hydrocarbon receptor in the recipient colon.
30010734	9	10	from	receptor	1942:1949	arg1	colon					1968:1972	the recipient colon	1954:1972	the recipient colon	1954:1972	In concordance with the metabolome data, metagenomics prediction analysis based on 16S rRNA gene sequencing also demonstrated that FMT modulated the metabolic functions of gut microbiota associated with indole alkaloid biosynthesis, cytochrome P450 and intestinal homeostasis, which coincided with up-regulation of cytokine interleukin-22 and enhanced activation of aryl hydrocarbon receptor in the recipient colon.
30010734	9	11	theme	cytochrome	1792:1801	arg1	P450					1803:1806	cytochrome P450	1792:1806	cytochrome P450	1792:1806	In concordance with the metabolome data, metagenomics prediction analysis based on 16S rRNA gene sequencing also demonstrated that FMT modulated the metabolic functions of gut microbiota associated with indole alkaloid biosynthesis, cytochrome P450 and intestinal homeostasis, which coincided with up-regulation of cytokine interleukin-22 and enhanced activation of aryl hydrocarbon receptor in the recipient colon.
30010734	10	12	dep	CONCLUSIONS	1975:1985	arg1	reveal					1996:2001	reveal	1996:2001	reveal a regulatory effect of FMT on tryptophan metabolism of gut microbiota in the recipient colon, which may play a potential role in maintenance of the intestinal barrier	1996:2168	CONCLUSIONS Our data reveal a regulatory effect of FMT on tryptophan metabolism of gut microbiota in the recipient colon, which may play a potential role in maintenance of the intestinal barrier.
30010734	8	13	from	acid	1532:1535	arg1	lumen					1552:1556	the colonic lumen	1540:1556	the colonic lumen	1540:1556	Metabolomic analysis revealed functional changes of the gut metabolome along with a significant increase of the typical microbiota-derived tryptophan catabolite indole-3-acetic acid in the colonic lumen.
30010734	2	14	theme	gastrointestinal	547:562	arg1	health					564:569	gastrointestinal health	547:569	gastrointestinal health	547:569	Using a piglet model, our previous study indicated that exogenous faecal microbiota can increase the expressions of tight junction proteins, mucin and antimicrobial peptide in the intestinal mucosa, suggesting a beneficial effect of FMT on gut barrier and gastrointestinal health.
30010734	2	15	theme	piglet	299:304	arg1	model					306:310	a piglet model	297:310	a piglet model	297:310	Using a piglet model, our previous study indicated that exogenous faecal microbiota can increase the expressions of tight junction proteins, mucin and antimicrobial peptide in the intestinal mucosa, suggesting a beneficial effect of FMT on gut barrier and gastrointestinal health.
30010734	10	16	theme	potential	2114:2122	arg1	role					2124:2127	a potential role	2112:2127	a potential role	2112:2127	CONCLUSIONS Our data reveal a regulatory effect of FMT on tryptophan metabolism of gut microbiota in the recipient colon, which may play a potential role in maintenance of the intestinal barrier.
30010734	0	17	theme	Piglet	146:151	arg1	Model					153:157	a Piglet Model	144:157	a Piglet Model	144:157	Faecal Microbiota Transplantation Reduces Susceptibility to Epithelial Injury and Modulates Tryptophan Metabolism of the Microbial Community in a Piglet Model.
30010734	3	18	theme	FMT-induced	610:620	arg1	changes					632:638	FMT-induced microbial changes	610:638	FMT-induced microbial changes	610:638	However, specific connections between FMT-induced microbial changes and modulation of the intestinal barrier remain to be fully illustrated.
30010734	5	19	from	influence	846:854	arg1	maintenance					870:880	the maintenance	866:880	the maintenance of intestinal homeostasis	866:906	METHODS The influence of FMT on the maintenance of intestinal homeostasis was assessed by early-life gut microbiota intervention on newborn piglets and subsequent lipopolysaccharide [LPS] challenge.
30010734	8	20	theme	colonic	1544:1550	arg1	lumen					1552:1556	the colonic lumen	1540:1556	the colonic lumen	1540:1556	Metabolomic analysis revealed functional changes of the gut metabolome along with a significant increase of the typical microbiota-derived tryptophan catabolite indole-3-acetic acid in the colonic lumen.
30010734	9	21	from	colon	1968:1972	arg1	activation					1911:1920	enhanced activation	1902:1920	enhanced activation of aryl hydrocarbon receptor in the recipient colon	1902:1972	In concordance with the metabolome data, metagenomics prediction analysis based on 16S rRNA gene sequencing also demonstrated that FMT modulated the metabolic functions of gut microbiota associated with indole alkaloid biosynthesis, cytochrome P450 and intestinal homeostasis, which coincided with up-regulation of cytokine interleukin-22 and enhanced activation of aryl hydrocarbon receptor in the recipient colon.
30010734	9	21	from	colon	1968:1972	arg1	up-regulation					1857:1869	up-regulation	1857:1869	up-regulation of cytokine interleukin-22	1857:1896	In concordance with the metabolome data, metagenomics prediction analysis based on 16S rRNA gene sequencing also demonstrated that FMT modulated the metabolic functions of gut microbiota associated with indole alkaloid biosynthesis, cytochrome P450 and intestinal homeostasis, which coincided with up-regulation of cytokine interleukin-22 and enhanced activation of aryl hydrocarbon receptor in the recipient colon.
30010734	9	22	theme	metabolome	1583:1592	arg1	data					1594:1597	the metabolome data	1579:1597	the metabolome data	1579:1597	In concordance with the metabolome data, metagenomics prediction analysis based on 16S rRNA gene sequencing also demonstrated that FMT modulated the metabolic functions of gut microbiota associated with indole alkaloid biosynthesis, cytochrome P450 and intestinal homeostasis, which coincided with up-regulation of cytokine interleukin-22 and enhanced activation of aryl hydrocarbon receptor in the recipient colon.
30010734	2	23	from	effect	514:519	arg1	barrier					535:541	gut barrier	531:541	gut barrier	531:541	Using a piglet model, our previous study indicated that exogenous faecal microbiota can increase the expressions of tight junction proteins, mucin and antimicrobial peptide in the intestinal mucosa, suggesting a beneficial effect of FMT on gut barrier and gastrointestinal health.
30010734	2	23	from	effect	514:519	arg1	health					564:569	gastrointestinal health	547:569	gastrointestinal health	547:569	Using a piglet model, our previous study indicated that exogenous faecal microbiota can increase the expressions of tight junction proteins, mucin and antimicrobial peptide in the intestinal mucosa, suggesting a beneficial effect of FMT on gut barrier and gastrointestinal health.
30010734	5	24	theme	gut	935:937	arg1	intervention					950:961	early-life gut microbiota intervention	924:961	early-life gut microbiota intervention on newborn piglets and subsequent lipopolysaccharide [LPS] challenge	924:1030	METHODS The influence of FMT on the maintenance of intestinal homeostasis was assessed by early-life gut microbiota intervention on newborn piglets and subsequent lipopolysaccharide [LPS] challenge.
30010734	9	25	from	up-regulation	1857:1869	arg1	colon					1968:1972	the recipient colon	1954:1972	the recipient colon	1954:1972	In concordance with the metabolome data, metagenomics prediction analysis based on 16S rRNA gene sequencing also demonstrated that FMT modulated the metabolic functions of gut microbiota associated with indole alkaloid biosynthesis, cytochrome P450 and intestinal homeostasis, which coincided with up-regulation of cytokine interleukin-22 and enhanced activation of aryl hydrocarbon receptor in the recipient colon.
30010734	2	26	theme	beneficial	503:512	arg1	effect					514:519	a beneficial effect	501:519	a beneficial effect of FMT on gut barrier and gastrointestinal health	501:569	Using a piglet model, our previous study indicated that exogenous faecal microbiota can increase the expressions of tight junction proteins, mucin and antimicrobial peptide in the intestinal mucosa, suggesting a beneficial effect of FMT on gut barrier and gastrointestinal health.
30010734	8	27	theme	functional	1385:1394	arg1	changes					1396:1402	functional changes	1385:1402	functional changes of the gut metabolome	1385:1424	Metabolomic analysis revealed functional changes of the gut metabolome along with a significant increase of the typical microbiota-derived tryptophan catabolite indole-3-acetic acid in the colonic lumen.
30010734	6	28	theme	gut	1049:1051	arg1	Analysis					1033:1040	Analysis	1033:1040	Analysis of the gut microbiome and metabolome	1033:1077	Analysis of the gut microbiome and metabolome was carried out by 16S rRNA gene sequencing and multiple mass spectrometry platforms.
30010734	9	29	theme	interleukin-22	1883:1896	arg1	activation					1911:1920	enhanced activation	1902:1920	enhanced activation of aryl hydrocarbon receptor in the recipient colon	1902:1972	In concordance with the metabolome data, metagenomics prediction analysis based on 16S rRNA gene sequencing also demonstrated that FMT modulated the metabolic functions of gut microbiota associated with indole alkaloid biosynthesis, cytochrome P450 and intestinal homeostasis, which coincided with up-regulation of cytokine interleukin-22 and enhanced activation of aryl hydrocarbon receptor in the recipient colon.
30010734	9	29	theme	interleukin-22	1883:1896	arg1	up-regulation					1857:1869	up-regulation	1857:1869	up-regulation of cytokine interleukin-22	1857:1896	In concordance with the metabolome data, metagenomics prediction analysis based on 16S rRNA gene sequencing also demonstrated that FMT modulated the metabolic functions of gut microbiota associated with indole alkaloid biosynthesis, cytochrome P450 and intestinal homeostasis, which coincided with up-regulation of cytokine interleukin-22 and enhanced activation of aryl hydrocarbon receptor in the recipient colon.
30010734	0	30	theme	Tryptophan	92:101	arg1	Metabolism					103:112	Tryptophan Metabolism	92:112	Tryptophan Metabolism of the Microbial Community in a Piglet Model	92:157	Faecal Microbiota Transplantation Reduces Susceptibility to Epithelial Injury and Modulates Tryptophan Metabolism of the Microbial Community in a Piglet Model.
30010734	9	31	theme	enhanced	1902:1909	arg1	activation					1911:1920	enhanced activation	1902:1920	enhanced activation of aryl hydrocarbon receptor in the recipient colon	1902:1972	In concordance with the metabolome data, metagenomics prediction analysis based on 16S rRNA gene sequencing also demonstrated that FMT modulated the metabolic functions of gut microbiota associated with indole alkaloid biosynthesis, cytochrome P450 and intestinal homeostasis, which coincided with up-regulation of cytokine interleukin-22 and enhanced activation of aryl hydrocarbon receptor in the recipient colon.
30010734	4	32	theme	function	773:780	arg1	role					755:758	the potential role	741:758	the potential role of metabolic function of gut microbiota in the beneficial effects of FMT	741:831	Here, we aimed to determine the potential role of metabolic function of gut microbiota in the beneficial effects of FMT.
30010734	1	33	dep	BACKGROUND	160:169	arg1	Faecal					180:185	Faecal	180:185	Faecal	180:185	BACKGROUND AND AIMS Faecal microbiota transplantation [FMT] has shown promise as a treatment for inflammatory bowel disease [IBD].
30010734	10	34	theme	intestinal	2151:2160	arg1	barrier					2162:2168	the intestinal barrier	2147:2168	the intestinal barrier	2147:2168	CONCLUSIONS Our data reveal a regulatory effect of FMT on tryptophan metabolism of gut microbiota in the recipient colon, which may play a potential role in maintenance of the intestinal barrier.
30010734	9	35	theme	16S	1642:1644	arg1	sequencing					1656:1665	16S rRNA gene sequencing	1642:1665	16S rRNA gene sequencing	1642:1665	In concordance with the metabolome data, metagenomics prediction analysis based on 16S rRNA gene sequencing also demonstrated that FMT modulated the metabolic functions of gut microbiota associated with indole alkaloid biosynthesis, cytochrome P450 and intestinal homeostasis, which coincided with up-regulation of cytokine interleukin-22 and enhanced activation of aryl hydrocarbon receptor in the recipient colon.
30010734	8	36	theme	Metabolomic	1355:1365	arg1	analysis					1367:1374	Metabolomic analysis	1355:1374	Metabolomic analysis	1355:1374	Metabolomic analysis revealed functional changes of the gut metabolome along with a significant increase of the typical microbiota-derived tryptophan catabolite indole-3-acetic acid in the colonic lumen.
30010734	4	37	theme	gut	785:787	arg1	microbiota					789:798	gut microbiota	785:798	gut microbiota	785:798	Here, we aimed to determine the potential role of metabolic function of gut microbiota in the beneficial effects of FMT.
30010734	0	38	theme	Microbial	121:129	arg1	Community					131:139	the Microbial Community	117:139	the Microbial Community	117:139	Faecal Microbiota Transplantation Reduces Susceptibility to Epithelial Injury and Modulates Tryptophan Metabolism of the Microbial Community in a Piglet Model.
30010734	5	39	theme	lipopolysaccharide	997:1014	arg1	challenge					1022:1030	subsequent lipopolysaccharide [LPS] challenge	986:1030	subsequent lipopolysaccharide [LPS] challenge	986:1030	METHODS The influence of FMT on the maintenance of intestinal homeostasis was assessed by early-life gut microbiota intervention on newborn piglets and subsequent lipopolysaccharide [LPS] challenge.
30010734	2	40	theme	peptide	456:462	arg1	expressions					392:402	the expressions	388:402	the expressions of tight junction proteins, mucin and antimicrobial peptide in the intestinal mucosa	388:487	Using a piglet model, our previous study indicated that exogenous faecal microbiota can increase the expressions of tight junction proteins, mucin and antimicrobial peptide in the intestinal mucosa, suggesting a beneficial effect of FMT on gut barrier and gastrointestinal health.
30010734	4	41	theme	potential	745:753	arg1	role					755:758	the potential role	741:758	the potential role of metabolic function of gut microbiota in the beneficial effects of FMT	741:831	Here, we aimed to determine the potential role of metabolic function of gut microbiota in the beneficial effects of FMT.
30010734	0	42	theme	Faecal	0:5	arg1	Transplantation					18:32	Faecal Microbiota Transplantation	0:32	Faecal Microbiota Transplantation	0:32	Faecal Microbiota Transplantation Reduces Susceptibility to Epithelial Injury and Modulates Tryptophan Metabolism of the Microbial Community in a Piglet Model.
30010734	9	43	with	concordance	1562:1572	arg1	data					1594:1597	the metabolome data	1579:1597	the metabolome data	1579:1597	In concordance with the metabolome data, metagenomics prediction analysis based on 16S rRNA gene sequencing also demonstrated that FMT modulated the metabolic functions of gut microbiota associated with indole alkaloid biosynthesis, cytochrome P450 and intestinal homeostasis, which coincided with up-regulation of cytokine interleukin-22 and enhanced activation of aryl hydrocarbon receptor in the recipient colon.
30010734	5	44	theme	newborn	966:972	arg1	piglets					974:980	newborn piglets	966:980	newborn piglets	966:980	METHODS The influence of FMT on the maintenance of intestinal homeostasis was assessed by early-life gut microbiota intervention on newborn piglets and subsequent lipopolysaccharide [LPS] challenge.
30010734	9	45	theme	recipient	1958:1966	arg1	colon					1968:1972	the recipient colon	1954:1972	the recipient colon	1954:1972	In concordance with the metabolome data, metagenomics prediction analysis based on 16S rRNA gene sequencing also demonstrated that FMT modulated the metabolic functions of gut microbiota associated with indole alkaloid biosynthesis, cytochrome P450 and intestinal homeostasis, which coincided with up-regulation of cytokine interleukin-22 and enhanced activation of aryl hydrocarbon receptor in the recipient colon.
30010734	3	46	theme	barrier	673:679	arg1	changes					632:638	FMT-induced microbial changes	610:638	FMT-induced microbial changes	610:638	However, specific connections between FMT-induced microbial changes and modulation of the intestinal barrier remain to be fully illustrated.
30010734	3	46	theme	barrier	673:679	arg1	modulation					644:653	modulation	644:653	modulation of the intestinal barrier	644:679	However, specific connections between FMT-induced microbial changes and modulation of the intestinal barrier remain to be fully illustrated.
30010734	2	47	theme	junction	413:420	arg1	proteins					422:429	tight junction proteins	407:429	tight junction proteins	407:429	Using a piglet model, our previous study indicated that exogenous faecal microbiota can increase the expressions of tight junction proteins, mucin and antimicrobial peptide in the intestinal mucosa, suggesting a beneficial effect of FMT on gut barrier and gastrointestinal health.
30010734	4	48	theme	FMT	829:831	arg1	effects					818:824	the beneficial effects	803:824	the beneficial effects of FMT	803:831	Here, we aimed to determine the potential role of metabolic function of gut microbiota in the beneficial effects of FMT.
30010734	1	49	dep	Faecal	180:185	arg1	transplantation					198:212	transplantation	198:212	transplantation	198:212	BACKGROUND AND AIMS Faecal microbiota transplantation [FMT] has shown promise as a treatment for inflammatory bowel disease [IBD].
30010734	1	49	dep	Faecal	180:185	arg1	[FMT					214:217	[FMT	214:217	[FMT	214:217	BACKGROUND AND AIMS Faecal microbiota transplantation [FMT] has shown promise as a treatment for inflammatory bowel disease [IBD].
30010734	1	49	dep	Faecal	180:185	arg1	microbiota					187:196	microbiota	187:196	microbiota	187:196	BACKGROUND AND AIMS Faecal microbiota transplantation [FMT] has shown promise as a treatment for inflammatory bowel disease [IBD].
30010734	0	50	theme	Epithelial	60:69	arg1	Injury					71:76	Epithelial Injury	60:76	Epithelial Injury	60:76	Faecal Microbiota Transplantation Reduces Susceptibility to Epithelial Injury and Modulates Tryptophan Metabolism of the Microbial Community in a Piglet Model.
30010734	7	51	theme	colonic	1220:1226	arg1	microbiota					1228:1237	colonic microbiota	1220:1237	colonic microbiota	1220:1237	RESULTS FMT modulated the diversity and composition of colonic microbiota and reduced the susceptibility to LPS-induced destruction of epithelial integrity and severe inflammatory response.
30010734	9	52	theme	metabolic	1708:1716	arg1	functions					1718:1726	the metabolic functions	1704:1726	the metabolic functions of gut microbiota associated with indole alkaloid biosynthesis, cytochrome P450 and intestinal homeostasis, which coincided with up-regulation of cytokine interleukin-22 and enhanced activation of aryl hydrocarbon receptor in the recipient colon	1704:1972	In concordance with the metabolome data, metagenomics prediction analysis based on 16S rRNA gene sequencing also demonstrated that FMT modulated the metabolic functions of gut microbiota associated with indole alkaloid biosynthesis, cytochrome P450 and intestinal homeostasis, which coincided with up-regulation of cytokine interleukin-22 and enhanced activation of aryl hydrocarbon receptor in the recipient colon.
30010734	7	53	theme	inflammatory	1332:1343	arg1	response					1345:1352	severe inflammatory response	1325:1352	severe inflammatory response	1325:1352	RESULTS FMT modulated the diversity and composition of colonic microbiota and reduced the susceptibility to LPS-induced destruction of epithelial integrity and severe inflammatory response.
30010734	8	54	theme	catabolite	1505:1514	arg1	acid					1532:1535	the typical microbiota-derived tryptophan catabolite indole-3-acetic acid	1463:1535	the typical microbiota-derived tryptophan catabolite indole-3-acetic acid in the colonic lumen	1463:1556	Metabolomic analysis revealed functional changes of the gut metabolome along with a significant increase of the typical microbiota-derived tryptophan catabolite indole-3-acetic acid in the colonic lumen.
30010734	6	55	theme	multiple	1127:1134	arg1	spectrometry					1141:1152	multiple mass spectrometry	1127:1152	multiple mass spectrometry	1127:1152	Analysis of the gut microbiome and metabolome was carried out by 16S rRNA gene sequencing and multiple mass spectrometry platforms.
30010734	10	56	theme	regulatory	2005:2014	arg1	effect					2016:2021	a regulatory effect	2003:2021	a regulatory effect of FMT on tryptophan metabolism of gut microbiota in the recipient colon, which may play a potential role in maintenance of the intestinal barrier	2003:2168	CONCLUSIONS Our data reveal a regulatory effect of FMT on tryptophan metabolism of gut microbiota in the recipient colon, which may play a potential role in maintenance of the intestinal barrier.
30010734	8	57	theme	acid	1532:1535	arg1	increase					1451:1458	a significant increase	1437:1458	a significant increase of the typical microbiota-derived tryptophan catabolite indole-3-acetic acid in the colonic lumen	1437:1556	Metabolomic analysis revealed functional changes of the gut metabolome along with a significant increase of the typical microbiota-derived tryptophan catabolite indole-3-acetic acid in the colonic lumen.
30010734	5	58	from	intervention	950:961	arg1	challenge					1022:1030	subsequent lipopolysaccharide [LPS] challenge	986:1030	subsequent lipopolysaccharide [LPS] challenge	986:1030	METHODS The influence of FMT on the maintenance of intestinal homeostasis was assessed by early-life gut microbiota intervention on newborn piglets and subsequent lipopolysaccharide [LPS] challenge.
30010734	5	58	from	intervention	950:961	arg1	piglets					974:980	newborn piglets	966:980	newborn piglets	966:980	METHODS The influence of FMT on the maintenance of intestinal homeostasis was assessed by early-life gut microbiota intervention on newborn piglets and subsequent lipopolysaccharide [LPS] challenge.
30010734	9	59	theme	microbiota	1735:1744	arg1	functions					1718:1726	the metabolic functions	1704:1726	the metabolic functions of gut microbiota associated with indole alkaloid biosynthesis, cytochrome P450 and intestinal homeostasis, which coincided with up-regulation of cytokine interleukin-22 and enhanced activation of aryl hydrocarbon receptor in the recipient colon	1704:1972	In concordance with the metabolome data, metagenomics prediction analysis based on 16S rRNA gene sequencing also demonstrated that FMT modulated the metabolic functions of gut microbiota associated with indole alkaloid biosynthesis, cytochrome P450 and intestinal homeostasis, which coincided with up-regulation of cytokine interleukin-22 and enhanced activation of aryl hydrocarbon receptor in the recipient colon.
30010734	7	60	theme	epithelial	1300:1309	arg1	integrity					1311:1319	epithelial integrity	1300:1319	epithelial integrity	1300:1319	RESULTS FMT modulated the diversity and composition of colonic microbiota and reduced the susceptibility to LPS-induced destruction of epithelial integrity and severe inflammatory response.
30010734	2	61	theme	faecal	357:362	arg1	microbiota					364:373	exogenous faecal microbiota	347:373	exogenous faecal microbiota	347:373	Using a piglet model, our previous study indicated that exogenous faecal microbiota can increase the expressions of tight junction proteins, mucin and antimicrobial peptide in the intestinal mucosa, suggesting a beneficial effect of FMT on gut barrier and gastrointestinal health.
30010734	1	62	theme	inflammatory	257:268	arg1	disease					276:282	inflammatory bowel disease	257:282	inflammatory bowel disease [IBD]	257:288	BACKGROUND AND AIMS Faecal microbiota transplantation [FMT] has shown promise as a treatment for inflammatory bowel disease [IBD].
30010734	6	63	theme	rRNA	1102:1105	arg1	sequencing					1112:1121	16S rRNA gene sequencing	1098:1121	16S rRNA gene sequencing	1098:1121	Analysis of the gut microbiome and metabolome was carried out by 16S rRNA gene sequencing and multiple mass spectrometry platforms.
30010734	8	64	theme	microbiota-derived	1475:1492	arg1	acid					1532:1535	the typical microbiota-derived tryptophan catabolite indole-3-acetic acid	1463:1535	the typical microbiota-derived tryptophan catabolite indole-3-acetic acid in the colonic lumen	1463:1556	Metabolomic analysis revealed functional changes of the gut metabolome along with a significant increase of the typical microbiota-derived tryptophan catabolite indole-3-acetic acid in the colonic lumen.
30010734	9	65	theme	alkaloid	1769:1776	arg1	biosynthesis					1778:1789	indole alkaloid biosynthesis	1762:1789	indole alkaloid biosynthesis	1762:1789	In concordance with the metabolome data, metagenomics prediction analysis based on 16S rRNA gene sequencing also demonstrated that FMT modulated the metabolic functions of gut microbiota associated with indole alkaloid biosynthesis, cytochrome P450 and intestinal homeostasis, which coincided with up-regulation of cytokine interleukin-22 and enhanced activation of aryl hydrocarbon receptor in the recipient colon.
30010734	5	66	theme	FMT	859:861	arg1	influence					846:854	The influence	842:854	The influence of FMT on the maintenance of intestinal homeostasis	842:906	METHODS The influence of FMT on the maintenance of intestinal homeostasis was assessed by early-life gut microbiota intervention on newborn piglets and subsequent lipopolysaccharide [LPS] challenge.
30010734	7	67	theme	RESULTS	1165:1171	arg1	FMT					1173:1175	RESULTS FMT	1165:1175	RESULTS FMT	1165:1175	RESULTS FMT modulated the diversity and composition of colonic microbiota and reduced the susceptibility to LPS-induced destruction of epithelial integrity and severe inflammatory response.
30010734	10	68	theme	gut	2058:2060	arg1	microbiota					2062:2071	gut microbiota	2058:2071	gut microbiota	2058:2071	CONCLUSIONS Our data reveal a regulatory effect of FMT on tryptophan metabolism of gut microbiota in the recipient colon, which may play a potential role in maintenance of the intestinal barrier.
30010734	6	69	dep	sequencing	1112:1121	arg1	platforms					1154:1162	platforms	1154:1162	platforms	1154:1162	Analysis of the gut microbiome and metabolome was carried out by 16S rRNA gene sequencing and multiple mass spectrometry platforms.
30010734	10	70	from	metabolism	2044:2053	arg1	colon					2090:2094	the recipient colon	2076:2094	the recipient colon	2076:2094	CONCLUSIONS Our data reveal a regulatory effect of FMT on tryptophan metabolism of gut microbiota in the recipient colon, which may play a potential role in maintenance of the intestinal barrier.
30010734	9	71	theme	intestinal	1812:1821	arg1	homeostasis					1823:1833	intestinal homeostasis	1812:1833	intestinal homeostasis	1812:1833	In concordance with the metabolome data, metagenomics prediction analysis based on 16S rRNA gene sequencing also demonstrated that FMT modulated the metabolic functions of gut microbiota associated with indole alkaloid biosynthesis, cytochrome P450 and intestinal homeostasis, which coincided with up-regulation of cytokine interleukin-22 and enhanced activation of aryl hydrocarbon receptor in the recipient colon.
30010734	0	72	from	Metabolism	103:112	arg1	Model					153:157	a Piglet Model	144:157	a Piglet Model	144:157	Faecal Microbiota Transplantation Reduces Susceptibility to Epithelial Injury and Modulates Tryptophan Metabolism of the Microbial Community in a Piglet Model.
30010734	5	73	dep	METHODS	834:840	arg1	assessed					912:919	assessed	912:919	was assessed by early-life gut microbiota intervention on newborn piglets and subsequent lipopolysaccharide [LPS] challenge	908:1030	METHODS The influence of FMT on the maintenance of intestinal homeostasis was assessed by early-life gut microbiota intervention on newborn piglets and subsequent lipopolysaccharide [LPS] challenge.
30010734	10	74	theme	recipient	2080:2088	arg1	colon					2090:2094	the recipient colon	2076:2094	the recipient colon	2076:2094	CONCLUSIONS Our data reveal a regulatory effect of FMT on tryptophan metabolism of gut microbiota in the recipient colon, which may play a potential role in maintenance of the intestinal barrier.
30010734	2	75	theme	gut	531:533	arg1	barrier					535:541	gut barrier	531:541	gut barrier	531:541	Using a piglet model, our previous study indicated that exogenous faecal microbiota can increase the expressions of tight junction proteins, mucin and antimicrobial peptide in the intestinal mucosa, suggesting a beneficial effect of FMT on gut barrier and gastrointestinal health.
30010734	3	76	theme	specific	581:588	arg1	connections					590:600	specific connections	581:600	specific connections between FMT-induced microbial changes and modulation of the intestinal barrier	581:679	However, specific connections between FMT-induced microbial changes and modulation of the intestinal barrier remain to be fully illustrated.
30010734	1	77	dep	disease	276:282	arg1	[IBD					284:287	[IBD	284:287	[IBD	284:287	BACKGROUND AND AIMS Faecal microbiota transplantation [FMT] has shown promise as a treatment for inflammatory bowel disease [IBD].
30010734	5	78	theme	early-life	924:933	arg1	intervention					950:961	early-life gut microbiota intervention	924:961	early-life gut microbiota intervention on newborn piglets and subsequent lipopolysaccharide [LPS] challenge	924:1030	METHODS The influence of FMT on the maintenance of intestinal homeostasis was assessed by early-life gut microbiota intervention on newborn piglets and subsequent lipopolysaccharide [LPS] challenge.
30010734	8	79	link	microbiota-derived	1475:1492	arg1	acid					1532:1535	the typical microbiota-derived tryptophan catabolite indole-3-acetic acid	1463:1535	the typical microbiota-derived tryptophan catabolite indole-3-acetic acid in the colonic lumen	1463:1556	Metabolomic analysis revealed functional changes of the gut metabolome along with a significant increase of the typical microbiota-derived tryptophan catabolite indole-3-acetic acid in the colonic lumen.
30010734	8	80	theme	gut	1411:1413	arg1	metabolome					1415:1424	the gut metabolome	1407:1424	the gut metabolome	1407:1424	Metabolomic analysis revealed functional changes of the gut metabolome along with a significant increase of the typical microbiota-derived tryptophan catabolite indole-3-acetic acid in the colonic lumen.
30010734	5	81	theme	intestinal	885:894	arg1	homeostasis					896:906	intestinal homeostasis	885:906	intestinal homeostasis	885:906	METHODS The influence of FMT on the maintenance of intestinal homeostasis was assessed by early-life gut microbiota intervention on newborn piglets and subsequent lipopolysaccharide [LPS] challenge.
30010734	7	82	theme	integrity	1311:1319	arg1	destruction					1285:1295	LPS-induced destruction	1273:1295	LPS-induced destruction of epithelial integrity and severe inflammatory response	1273:1352	RESULTS FMT modulated the diversity and composition of colonic microbiota and reduced the susceptibility to LPS-induced destruction of epithelial integrity and severe inflammatory response.
30010734	8	83	from	lumen	1552:1556	arg1	increase					1451:1458	a significant increase	1437:1458	a significant increase of the typical microbiota-derived tryptophan catabolite indole-3-acetic acid in the colonic lumen	1437:1556	Metabolomic analysis revealed functional changes of the gut metabolome along with a significant increase of the typical microbiota-derived tryptophan catabolite indole-3-acetic acid in the colonic lumen.
30010734	3	84	theme	microbial	622:630	arg1	changes					632:638	FMT-induced microbial changes	610:638	FMT-induced microbial changes	610:638	However, specific connections between FMT-induced microbial changes and modulation of the intestinal barrier remain to be fully illustrated.
30010734	9	85	theme	metagenomics	1600:1611	arg1	analysis					1624:1631	metagenomics prediction analysis	1600:1631	metagenomics prediction analysis based on 16S rRNA gene sequencing	1600:1665	In concordance with the metabolome data, metagenomics prediction analysis based on 16S rRNA gene sequencing also demonstrated that FMT modulated the metabolic functions of gut microbiota associated with indole alkaloid biosynthesis, cytochrome P450 and intestinal homeostasis, which coincided with up-regulation of cytokine interleukin-22 and enhanced activation of aryl hydrocarbon receptor in the recipient colon.
30010734	9	86	theme	cytokine	1874:1881	arg1	interleukin-22					1883:1896	cytokine interleukin-22	1874:1896	cytokine interleukin-22	1874:1896	In concordance with the metabolome data, metagenomics prediction analysis based on 16S rRNA gene sequencing also demonstrated that FMT modulated the metabolic functions of gut microbiota associated with indole alkaloid biosynthesis, cytochrome P450 and intestinal homeostasis, which coincided with up-regulation of cytokine interleukin-22 and enhanced activation of aryl hydrocarbon receptor in the recipient colon.
30010734	5	87	theme	homeostasis	896:906	arg1	maintenance					870:880	the maintenance	866:880	the maintenance of intestinal homeostasis	866:906	METHODS The influence of FMT on the maintenance of intestinal homeostasis was assessed by early-life gut microbiota intervention on newborn piglets and subsequent lipopolysaccharide [LPS] challenge.
30010734	7	88	dep	diversity	1191:1199	arg1	the					1187:1189	the	1187:1189	the	1187:1189	RESULTS FMT modulated the diversity and composition of colonic microbiota and reduced the susceptibility to LPS-induced destruction of epithelial integrity and severe inflammatory response.
30010734	10	89	theme	barrier	2162:2168	arg1	maintenance					2132:2142	maintenance	2132:2142	maintenance of the intestinal barrier	2132:2168	CONCLUSIONS Our data reveal a regulatory effect of FMT on tryptophan metabolism of gut microbiota in the recipient colon, which may play a potential role in maintenance of the intestinal barrier.
30010734	7	90	theme	microbiota	1228:1237	arg1	diversity					1191:1199	diversity	1191:1199	diversity	1191:1199	RESULTS FMT modulated the diversity and composition of colonic microbiota and reduced the susceptibility to LPS-induced destruction of epithelial integrity and severe inflammatory response.
30010734	7	90	theme	microbiota	1228:1237	arg1	composition					1205:1215	composition	1205:1215	composition	1205:1215	RESULTS FMT modulated the diversity and composition of colonic microbiota and reduced the susceptibility to LPS-induced destruction of epithelial integrity and severe inflammatory response.
30010734	4	91	theme	microbiota	789:798	arg1	function					773:780	metabolic function	763:780	metabolic function of gut microbiota	763:798	Here, we aimed to determine the potential role of metabolic function of gut microbiota in the beneficial effects of FMT.
30010734	2	92	theme	FMT	524:526	arg1	effect					514:519	a beneficial effect	501:519	a beneficial effect of FMT on gut barrier and gastrointestinal health	501:569	Using a piglet model, our previous study indicated that exogenous faecal microbiota can increase the expressions of tight junction proteins, mucin and antimicrobial peptide in the intestinal mucosa, suggesting a beneficial effect of FMT on gut barrier and gastrointestinal health.
30010734	4	93	theme	metabolic	763:771	arg1	function					773:780	metabolic function	763:780	metabolic function of gut microbiota	763:798	Here, we aimed to determine the potential role of metabolic function of gut microbiota in the beneficial effects of FMT.
30010734	0	94	theme	Community	131:139	arg1	Metabolism					103:112	Tryptophan Metabolism	92:112	Tryptophan Metabolism of the Microbial Community in a Piglet Model	92:157	Faecal Microbiota Transplantation Reduces Susceptibility to Epithelial Injury and Modulates Tryptophan Metabolism of the Microbial Community in a Piglet Model.
30010734	5	95	theme	subsequent	986:995	arg1	challenge					1022:1030	subsequent lipopolysaccharide [LPS] challenge	986:1030	subsequent lipopolysaccharide [LPS] challenge	986:1030	METHODS The influence of FMT on the maintenance of intestinal homeostasis was assessed by early-life gut microbiota intervention on newborn piglets and subsequent lipopolysaccharide [LPS] challenge.
30010734	6	96	theme	16S	1098:1100	arg1	sequencing					1112:1121	16S rRNA gene sequencing	1098:1121	16S rRNA gene sequencing	1098:1121	Analysis of the gut microbiome and metabolome was carried out by 16S rRNA gene sequencing and multiple mass spectrometry platforms.
30010734	9	97	theme	aryl	1925:1928	arg1	receptor					1942:1949	aryl hydrocarbon receptor	1925:1949	aryl hydrocarbon receptor in the recipient colon	1925:1972	In concordance with the metabolome data, metagenomics prediction analysis based on 16S rRNA gene sequencing also demonstrated that FMT modulated the metabolic functions of gut microbiota associated with indole alkaloid biosynthesis, cytochrome P450 and intestinal homeostasis, which coincided with up-regulation of cytokine interleukin-22 and enhanced activation of aryl hydrocarbon receptor in the recipient colon.
30010734	6	98	dep	gut	1049:1051	arg1	metabolome					1068:1077	metabolome	1068:1077	metabolome	1068:1077	Analysis of the gut microbiome and metabolome was carried out by 16S rRNA gene sequencing and multiple mass spectrometry platforms.
30010734	6	98	dep	gut	1049:1051	arg1	microbiome					1053:1062	microbiome	1053:1062	microbiome	1053:1062	Analysis of the gut microbiome and metabolome was carried out by 16S rRNA gene sequencing and multiple mass spectrometry platforms.
30010734	8	99	theme	metabolome	1415:1424	arg1	changes					1396:1402	functional changes	1385:1402	functional changes of the gut metabolome	1385:1424	Metabolomic analysis revealed functional changes of the gut metabolome along with a significant increase of the typical microbiota-derived tryptophan catabolite indole-3-acetic acid in the colonic lumen.
30010734	0	100	theme	Microbiota	7:16	arg1	Transplantation					18:32	Faecal Microbiota Transplantation	0:32	Faecal Microbiota Transplantation	0:32	Faecal Microbiota Transplantation Reduces Susceptibility to Epithelial Injury and Modulates Tryptophan Metabolism of the Microbial Community in a Piglet Model.
30010734	4	101	from	role	755:758	arg1	effects					818:824	the beneficial effects	803:824	the beneficial effects of FMT	803:831	Here, we aimed to determine the potential role of metabolic function of gut microbiota in the beneficial effects of FMT.
30010734	5	102	theme	microbiota	939:948	arg1	intervention					950:961	early-life gut microbiota intervention	924:961	early-life gut microbiota intervention on newborn piglets and subsequent lipopolysaccharide [LPS] challenge	924:1030	METHODS The influence of FMT on the maintenance of intestinal homeostasis was assessed by early-life gut microbiota intervention on newborn piglets and subsequent lipopolysaccharide [LPS] challenge.
30010734	2	103	theme	antimicrobial	442:454	arg1	peptide					456:462	antimicrobial peptide	442:462	antimicrobial peptide	442:462	Using a piglet model, our previous study indicated that exogenous faecal microbiota can increase the expressions of tight junction proteins, mucin and antimicrobial peptide in the intestinal mucosa, suggesting a beneficial effect of FMT on gut barrier and gastrointestinal health.
30010734	4	104	theme	beneficial	807:816	arg1	effects					818:824	the beneficial effects	803:824	the beneficial effects of FMT	803:831	Here, we aimed to determine the potential role of metabolic function of gut microbiota in the beneficial effects of FMT.
30010734	2	105	theme	mucin	432:436	arg1	expressions					392:402	the expressions	388:402	the expressions of tight junction proteins, mucin and antimicrobial peptide in the intestinal mucosa	388:487	Using a piglet model, our previous study indicated that exogenous faecal microbiota can increase the expressions of tight junction proteins, mucin and antimicrobial peptide in the intestinal mucosa, suggesting a beneficial effect of FMT on gut barrier and gastrointestinal health.
30010734	9	106	theme	rRNA	1646:1649	arg1	sequencing					1656:1665	16S rRNA gene sequencing	1642:1665	16S rRNA gene sequencing	1642:1665	In concordance with the metabolome data, metagenomics prediction analysis based on 16S rRNA gene sequencing also demonstrated that FMT modulated the metabolic functions of gut microbiota associated with indole alkaloid biosynthesis, cytochrome P450 and intestinal homeostasis, which coincided with up-regulation of cytokine interleukin-22 and enhanced activation of aryl hydrocarbon receptor in the recipient colon.
30010734	3	107	theme	intestinal	662:671	arg1	barrier					673:679	the intestinal barrier	658:679	the intestinal barrier	658:679	However, specific connections between FMT-induced microbial changes and modulation of the intestinal barrier remain to be fully illustrated.
30010734	8	108	theme	significant	1439:1449	arg1	increase					1451:1458	a significant increase	1437:1458	a significant increase of the typical microbiota-derived tryptophan catabolite indole-3-acetic acid in the colonic lumen	1437:1556	Metabolomic analysis revealed functional changes of the gut metabolome along with a significant increase of the typical microbiota-derived tryptophan catabolite indole-3-acetic acid in the colonic lumen.
30010734	2	109	theme	proteins	422:429	arg1	expressions					392:402	the expressions	388:402	the expressions of tight junction proteins, mucin and antimicrobial peptide in the intestinal mucosa	388:487	Using a piglet model, our previous study indicated that exogenous faecal microbiota can increase the expressions of tight junction proteins, mucin and antimicrobial peptide in the intestinal mucosa, suggesting a beneficial effect of FMT on gut barrier and gastrointestinal health.
30010734	7	110	theme	response	1345:1352	arg1	destruction					1285:1295	LPS-induced destruction	1273:1295	LPS-induced destruction of epithelial integrity and severe inflammatory response	1273:1352	RESULTS FMT modulated the diversity and composition of colonic microbiota and reduced the susceptibility to LPS-induced destruction of epithelial integrity and severe inflammatory response.
30010734	2	111	theme	intestinal	471:480	arg1	mucosa					482:487	the intestinal mucosa	467:487	the intestinal mucosa	467:487	Using a piglet model, our previous study indicated that exogenous faecal microbiota can increase the expressions of tight junction proteins, mucin and antimicrobial peptide in the intestinal mucosa, suggesting a beneficial effect of FMT on gut barrier and gastrointestinal health.
30010734	2	112	theme	tight	407:411	arg1	proteins					422:429	tight junction proteins	407:429	tight junction proteins	407:429	Using a piglet model, our previous study indicated that exogenous faecal microbiota can increase the expressions of tight junction proteins, mucin and antimicrobial peptide in the intestinal mucosa, suggesting a beneficial effect of FMT on gut barrier and gastrointestinal health.
30010734	9	113	from	activation	1911:1920	arg1	colon					1968:1972	the recipient colon	1954:1972	the recipient colon	1954:1972	In concordance with the metabolome data, metagenomics prediction analysis based on 16S rRNA gene sequencing also demonstrated that FMT modulated the metabolic functions of gut microbiota associated with indole alkaloid biosynthesis, cytochrome P450 and intestinal homeostasis, which coincided with up-regulation of cytokine interleukin-22 and enhanced activation of aryl hydrocarbon receptor in the recipient colon.
30010734	7	114	theme	severe	1325:1330	arg1	response					1345:1352	severe inflammatory response	1325:1352	severe inflammatory response	1325:1352	RESULTS FMT modulated the diversity and composition of colonic microbiota and reduced the susceptibility to LPS-induced destruction of epithelial integrity and severe inflammatory response.
30010734	10	115	theme	FMT	2026:2028	arg1	effect					2016:2021	a regulatory effect	2003:2021	a regulatory effect of FMT on tryptophan metabolism of gut microbiota in the recipient colon, which may play a potential role in maintenance of the intestinal barrier	2003:2168	CONCLUSIONS Our data reveal a regulatory effect of FMT on tryptophan metabolism of gut microbiota in the recipient colon, which may play a potential role in maintenance of the intestinal barrier.
30010734	8	116	theme	typical	1467:1473	arg1	acid					1532:1535	the typical microbiota-derived tryptophan catabolite indole-3-acetic acid	1463:1535	the typical microbiota-derived tryptophan catabolite indole-3-acetic acid in the colonic lumen	1463:1556	Metabolomic analysis revealed functional changes of the gut metabolome along with a significant increase of the typical microbiota-derived tryptophan catabolite indole-3-acetic acid in the colonic lumen.
30010734	5	117	theme	[LPS	1016:1019	arg1	challenge					1022:1030	subsequent lipopolysaccharide [LPS] challenge	986:1030	subsequent lipopolysaccharide [LPS] challenge	986:1030	METHODS The influence of FMT on the maintenance of intestinal homeostasis was assessed by early-life gut microbiota intervention on newborn piglets and subsequent lipopolysaccharide [LPS] challenge.
30010734	2	118	from	expressions	392:402	arg1	mucosa					482:487	the intestinal mucosa	467:487	the intestinal mucosa	467:487	Using a piglet model, our previous study indicated that exogenous faecal microbiota can increase the expressions of tight junction proteins, mucin and antimicrobial peptide in the intestinal mucosa, suggesting a beneficial effect of FMT on gut barrier and gastrointestinal health.
30010734	6	119	theme	mass	1136:1139	arg1	spectrometry					1141:1152	multiple mass spectrometry	1127:1152	multiple mass spectrometry	1127:1152	Analysis of the gut microbiome and metabolome was carried out by 16S rRNA gene sequencing and multiple mass spectrometry platforms.
30010734	9	120	theme	gene	1651:1654	arg1	sequencing					1656:1665	16S rRNA gene sequencing	1642:1665	16S rRNA gene sequencing	1642:1665	In concordance with the metabolome data, metagenomics prediction analysis based on 16S rRNA gene sequencing also demonstrated that FMT modulated the metabolic functions of gut microbiota associated with indole alkaloid biosynthesis, cytochrome P450 and intestinal homeostasis, which coincided with up-regulation of cytokine interleukin-22 and enhanced activation of aryl hydrocarbon receptor in the recipient colon.
30010734	8	121	theme	tryptophan	1494:1503	arg1	acid					1532:1535	the typical microbiota-derived tryptophan catabolite indole-3-acetic acid	1463:1535	the typical microbiota-derived tryptophan catabolite indole-3-acetic acid in the colonic lumen	1463:1556	Metabolomic analysis revealed functional changes of the gut metabolome along with a significant increase of the typical microbiota-derived tryptophan catabolite indole-3-acetic acid in the colonic lumen.
30010734	2	122	theme	previous	317:324	arg1	study					326:330	our previous study	313:330	our previous study	313:330	Using a piglet model, our previous study indicated that exogenous faecal microbiota can increase the expressions of tight junction proteins, mucin and antimicrobial peptide in the intestinal mucosa, suggesting a beneficial effect of FMT on gut barrier and gastrointestinal health.
30010734	9	123	theme	gut	1731:1733	arg1	microbiota					1735:1744	gut microbiota	1731:1744	gut microbiota associated with indole alkaloid biosynthesis, cytochrome P450 and intestinal homeostasis, which coincided with up-regulation of cytokine interleukin-22 and enhanced activation of aryl hydrocarbon receptor in the recipient colon	1731:1972	In concordance with the metabolome data, metagenomics prediction analysis based on 16S rRNA gene sequencing also demonstrated that FMT modulated the metabolic functions of gut microbiota associated with indole alkaloid biosynthesis, cytochrome P450 and intestinal homeostasis, which coincided with up-regulation of cytokine interleukin-22 and enhanced activation of aryl hydrocarbon receptor in the recipient colon.
30010734	8	124	theme	indole-3-acetic	1516:1530	arg1	acid					1532:1535	the typical microbiota-derived tryptophan catabolite indole-3-acetic acid	1463:1535	the typical microbiota-derived tryptophan catabolite indole-3-acetic acid in the colonic lumen	1463:1556	Metabolomic analysis revealed functional changes of the gut metabolome along with a significant increase of the typical microbiota-derived tryptophan catabolite indole-3-acetic acid in the colonic lumen.
30010734	9	125	theme	hydrocarbon	1930:1940	arg1	receptor					1942:1949	aryl hydrocarbon receptor	1925:1949	aryl hydrocarbon receptor in the recipient colon	1925:1972	In concordance with the metabolome data, metagenomics prediction analysis based on 16S rRNA gene sequencing also demonstrated that FMT modulated the metabolic functions of gut microbiota associated with indole alkaloid biosynthesis, cytochrome P450 and intestinal homeostasis, which coincided with up-regulation of cytokine interleukin-22 and enhanced activation of aryl hydrocarbon receptor in the recipient colon.
30010734	8	126	from	increase	1451:1458	arg1	lumen					1552:1556	the colonic lumen	1540:1556	the colonic lumen	1540:1556	Metabolomic analysis revealed functional changes of the gut metabolome along with a significant increase of the typical microbiota-derived tryptophan catabolite indole-3-acetic acid in the colonic lumen.
30868059	12	0	theme	interest	2094:2101	arg1	finding					2110:2116	our finding that lysozyme activity against Gram-negative bacteria is enhanced in the presence of glycine and charged amino acids over a wide range of concentrations	2106:2269	our finding that lysozyme activity against Gram-negative bacteria is enhanced in the presence of glycine and charged amino acids over a wide range of concentrations	2106:2269	Of particular interest is our finding that lysozyme activity against Gram-negative bacteria is enhanced in the presence of glycine and charged amino acids over a wide range of concentrations.
30868059	7	1	theme	amino	1275:1279	arg1	acids					1281:1285	amino acids	1275:1285	amino acids	1275:1285	When using immobilized lysozyme, the effects of amino acids on the rate of cell lysis are significantly reduced.
30868059	11	2	theme	immobilized	1917:1927	arg1	lysozyme					1929:1936	native and immobilized lysozyme	1906:1936	lysozyme	1929:1936	A greater understanding of the effect of amino acids on the activity of native and immobilized lysozyme is important for both the development of new materials for medical purposes and elucidating the interaction of lysozyme with bacterial cells.
30868059	9	3	theme	Gram-positive	1617:1629	arg1	bacteria					1649:1656	both Gram-positive and Gram-negative bacteria	1612:1656	both Gram-positive and Gram-negative bacteria	1612:1656	The broadening of the pH optimum of lysozyme activity after immobilization has been demonstrated for both Gram-positive and Gram-negative bacteria.
30868059	3	4	theme	immobilized	578:588	arg1	preparations					590:601	such immobilized preparations	573:601	such immobilized preparations	573:601	However, information on the effect of such immobilized preparations on whole bacterial cells is quite limited.
30868059	11	5	theme	bacterial	2063:2071	arg1	cells					2073:2077	bacterial cells	2063:2077	bacterial cells	2063:2077	A greater understanding of the effect of amino acids on the activity of native and immobilized lysozyme is important for both the development of new materials for medical purposes and elucidating the interaction of lysozyme with bacterial cells.
30868059	11	6	from	understanding	1844:1856	arg1	activity					1894:1901	the activity	1890:1901	the activity of native and immobilized lysozyme	1890:1936	A greater understanding of the effect of amino acids on the activity of native and immobilized lysozyme is important for both the development of new materials for medical purposes and elucidating the interaction of lysozyme with bacterial cells.
30868059	4	7	theme	glycine	695:701	arg1	acids					740:744	glycine and charged (basic and acidic) amino acids	695:744	glycine and charged (basic and acidic) amino acids	695:744	Here, we demonstrate the differential effects of glycine and charged (basic and acidic) amino acids on the enzymatic lysis of Gram-positive and Gram-negative bacteria by soluble and immobilized lysozyme.
30868059	4	8	theme	charged	707:713	arg1	acids					740:744	glycine and charged (basic and acidic) amino acids	695:744	glycine and charged (basic and acidic) amino acids	695:744	Here, we demonstrate the differential effects of glycine and charged (basic and acidic) amino acids on the enzymatic lysis of Gram-positive and Gram-negative bacteria by soluble and immobilized lysozyme.
30868059	10	9	theme	Michaelis	1663:1671	arg1	Km					1683:1684	Km	1683:1684	Km	1683:1684	The Michaelis constant (Km) values of immobilized lysozyme were increased by 1.5-fold for E. coli cell lysis and 4.6-fold for M. luteus cell lysis compared to soluble enzyme.
30868059	10	9	theme	Michaelis	1663:1671	arg1	constant					1673:1680	Michaelis constant	1663:1680	The Michaelis constant (Km) values of immobilized lysozyme	1659:1716	The Michaelis constant (Km) values of immobilized lysozyme were increased by 1.5-fold for E. coli cell lysis and 4.6-fold for M. luteus cell lysis compared to soluble enzyme.
30868059	3	10	theme	bacterial	612:620	arg1	cells					622:626	whole bacterial cells	606:626	whole bacterial cells	606:626	However, information on the effect of such immobilized preparations on whole bacterial cells is quite limited.
30868059	4	11	from	effects	684:690	arg1	lysis					763:767	the enzymatic lysis	749:767	the enzymatic lysis of Gram-positive and Gram-negative bacteria by soluble and immobilized lysozyme	749:847	Here, we demonstrate the differential effects of glycine and charged (basic and acidic) amino acids on the enzymatic lysis of Gram-positive and Gram-negative bacteria by soluble and immobilized lysozyme.
30868059	1	12	theme	new	202:204	arg1	strains					237:243	new antibiotic-resistant bacterial strains	202:243	new antibiotic-resistant bacterial strains means it	202:252	The emergence of new antibiotic-resistant bacterial strains means it is increasingly important to find alternatives to traditional antibiotics, such as bacteriolytic enzymes.
30868059	8	13	from	concentrations	1445:1458	arg1	kinetics					1423:1430	cell lysis kinetics	1412:1430	cell lysis kinetics at bacterial concentrations below 4 × 108 colony-forming units·mL-1	1412:1498	For immobilized lysozyme, the presence of an external diffusion mode on cell lysis kinetics at bacterial concentrations below 4 × 108 colony-forming units·mL-1 was shown.
30868059	5	14	theme	amino	868:872	arg1	acids					874:878	basic amino acids	862:878	basic amino acids (histidine, lysine, and arginine)	862:912	Glycine and basic amino acids (histidine, lysine, and arginine) significantly increase the rate of lysis of Gram-negative Escherichia coli cells in the presence of soluble lysozyme, but they do not substantially affect the rate of enzymatic lysis of Gram-positive Micrococcus luteus.
30868059	5	14	theme	amino	868:872	arg1	lysine					892:897	lysine	892:897	lysine	892:897	Glycine and basic amino acids (histidine, lysine, and arginine) significantly increase the rate of lysis of Gram-negative Escherichia coli cells in the presence of soluble lysozyme, but they do not substantially affect the rate of enzymatic lysis of Gram-positive Micrococcus luteus.
30868059	5	14	theme	amino	868:872	arg1	arginine					904:911	arginine	904:911	arginine	904:911	Glycine and basic amino acids (histidine, lysine, and arginine) significantly increase the rate of lysis of Gram-negative Escherichia coli cells in the presence of soluble lysozyme, but they do not substantially affect the rate of enzymatic lysis of Gram-positive Micrococcus luteus.
30868059	5	14	theme	amino	868:872	arg1	histidine					881:889	histidine	881:889	histidine	881:889	Glycine and basic amino acids (histidine, lysine, and arginine) significantly increase the rate of lysis of Gram-negative Escherichia coli cells in the presence of soluble lysozyme, but they do not substantially affect the rate of enzymatic lysis of Gram-positive Micrococcus luteus.
30868059	11	15	theme	acids	1881:1885	arg1	effect					1865:1870	the effect	1861:1870	the effect of amino acids on the activity of native and immobilized lysozyme	1861:1936	A greater understanding of the effect of amino acids on the activity of native and immobilized lysozyme is important for both the development of new materials for medical purposes and elucidating the interaction of lysozyme with bacterial cells.
30868059	0	16	theme	charged	152:158	arg1	acids					166:170	charged amino acids	152:170	charged amino acids	152:170	The bacteriolytic activity of native and covalently immobilized lysozyme against Gram-positive and Gram-negative bacteria is differentially affected by charged amino acids and glycine.
30868059	5	17	theme	lysis	1091:1095	arg1	rate					1073:1076	the rate	1069:1076	the rate of enzymatic lysis of Gram-positive Micrococcus luteus	1069:1131	Glycine and basic amino acids (histidine, lysine, and arginine) significantly increase the rate of lysis of Gram-negative Escherichia coli cells in the presence of soluble lysozyme, but they do not substantially affect the rate of enzymatic lysis of Gram-positive Micrococcus luteus.
30868059	4	18	theme	amino	734:738	arg1	acids					740:744	glycine and charged (basic and acidic) amino acids	695:744	glycine and charged (basic and acidic) amino acids	695:744	Here, we demonstrate the differential effects of glycine and charged (basic and acidic) amino acids on the enzymatic lysis of Gram-positive and Gram-negative bacteria by soluble and immobilized lysozyme.
30868059	11	19	theme	medical	1997:2003	arg1	purposes					2005:2012	medical purposes	1997:2012	medical purposes	1997:2012	A greater understanding of the effect of amino acids on the activity of native and immobilized lysozyme is important for both the development of new materials for medical purposes and elucidating the interaction of lysozyme with bacterial cells.
30868059	9	20	theme	optimum	1536:1542	arg1	broadening					1515:1524	The broadening	1511:1524	The broadening of the pH optimum of lysozyme activity after immobilization	1511:1584	The broadening of the pH optimum of lysozyme activity after immobilization has been demonstrated for both Gram-positive and Gram-negative bacteria.
30868059	12	21	theme	Gram-negative	2149:2161	arg1	bacteria					2163:2170	Gram-negative bacteria	2149:2170	Gram-negative bacteria	2149:2170	Of particular interest is our finding that lysozyme activity against Gram-negative bacteria is enhanced in the presence of glycine and charged amino acids over a wide range of concentrations.
30868059	5	22	theme	Gram-negative	958:970	arg1	cells					989:993	Gram-negative Escherichia coli cells	958:993	Gram-negative Escherichia coli cells	958:993	Glycine and basic amino acids (histidine, lysine, and arginine) significantly increase the rate of lysis of Gram-negative Escherichia coli cells in the presence of soluble lysozyme, but they do not substantially affect the rate of enzymatic lysis of Gram-positive Micrococcus luteus.
30868059	10	23	theme	soluble	1818:1824	arg1	enzyme					1826:1831	soluble enzyme	1818:1831	soluble enzyme	1818:1831	The Michaelis constant (Km) values of immobilized lysozyme were increased by 1.5-fold for E. coli cell lysis and 4.6-fold for M. luteus cell lysis compared to soluble enzyme.
30868059	9	24	theme	lysozyme	1547:1554	arg1	activity					1556:1563	lysozyme activity	1547:1563	lysozyme activity	1547:1563	The broadening of the pH optimum of lysozyme activity after immobilization has been demonstrated for both Gram-positive and Gram-negative bacteria.
30868059	11	25	theme	new	1979:1981	arg1	materials					1983:1991	new materials	1979:1991	new materials for medical purposes	1979:2012	A greater understanding of the effect of amino acids on the activity of native and immobilized lysozyme is important for both the development of new materials for medical purposes and elucidating the interaction of lysozyme with bacterial cells.
30868059	8	26	theme	immobilized	1344:1354	arg1	lysozyme					1356:1363	immobilized lysozyme	1344:1363	immobilized lysozyme	1344:1363	For immobilized lysozyme, the presence of an external diffusion mode on cell lysis kinetics at bacterial concentrations below 4 × 108 colony-forming units·mL-1 was shown.
30868059	8	27	attach	presence	1370:1377	arg1	kinetics					1423:1430	cell lysis kinetics	1412:1430	cell lysis kinetics at bacterial concentrations below 4 × 108 colony-forming units·mL-1	1412:1498	For immobilized lysozyme, the presence of an external diffusion mode on cell lysis kinetics at bacterial concentrations below 4 × 108 colony-forming units·mL-1 was shown.
30868059	8	27	attach	presence	1370:1377	arg2	mode					1404:1407	an external diffusion mode	1382:1407	an external diffusion mode	1382:1407	For immobilized lysozyme, the presence of an external diffusion mode on cell lysis kinetics at bacterial concentrations below 4 × 108 colony-forming units·mL-1 was shown.
30868059	2	28	theme	applications	521:532	arg1	lysozyme					385:392	The bacteriolytic enzyme lysozyme	360:392	The bacteriolytic enzyme lysozyme	360:392	The bacteriolytic enzyme lysozyme is widely used in medicine as an antimicrobial agent, and covalent immobilization of lysozyme can expand its range of possible applications.
30868059	2	28	theme	applications	521:532	arg1	range					503:507	its range	499:507	its range of possible applications	499:532	The bacteriolytic enzyme lysozyme is widely used in medicine as an antimicrobial agent, and covalent immobilization of lysozyme can expand its range of possible applications.
30868059	2	28	theme	applications	521:532	arg1	agent					441:445	an antimicrobial agent	424:445	an antimicrobial agent	424:445	The bacteriolytic enzyme lysozyme is widely used in medicine as an antimicrobial agent, and covalent immobilization of lysozyme can expand its range of possible applications.
30868059	0	29	theme	Gram-positive	81:93	arg1	bacteria					113:120	Gram-positive and Gram-negative bacteria	81:120	Gram-positive and Gram-negative bacteria	81:120	The bacteriolytic activity of native and covalently immobilized lysozyme against Gram-positive and Gram-negative bacteria is differentially affected by charged amino acids and glycine.
30868059	0	30	theme	Gram-negative	99:111	arg1	bacteria					113:120	Gram-positive and Gram-negative bacteria	81:120	Gram-positive and Gram-negative bacteria	81:120	The bacteriolytic activity of native and covalently immobilized lysozyme against Gram-positive and Gram-negative bacteria is differentially affected by charged amino acids and glycine.
30868059	5	31	theme	Gram-positive	1100:1112	arg1	Micrococcus luteus					1114:1131	Gram-positive Micrococcus luteus	1100:1131	Gram-positive Micrococcus luteus	1100:1131	Glycine and basic amino acids (histidine, lysine, and arginine) significantly increase the rate of lysis of Gram-negative Escherichia coli cells in the presence of soluble lysozyme, but they do not substantially affect the rate of enzymatic lysis of Gram-positive Micrococcus luteus.
30868059	4	32	theme	bacteria	804:811	arg1	lysis					763:767	the enzymatic lysis	749:767	the enzymatic lysis of Gram-positive and Gram-negative bacteria by soluble and immobilized lysozyme	749:847	Here, we demonstrate the differential effects of glycine and charged (basic and acidic) amino acids on the enzymatic lysis of Gram-positive and Gram-negative bacteria by soluble and immobilized lysozyme.
30868059	2	33	theme	antimicrobial	427:439	arg1	lysozyme					385:392	The bacteriolytic enzyme lysozyme	360:392	The bacteriolytic enzyme lysozyme	360:392	The bacteriolytic enzyme lysozyme is widely used in medicine as an antimicrobial agent, and covalent immobilization of lysozyme can expand its range of possible applications.
30868059	2	33	theme	antimicrobial	427:439	arg1	range					503:507	its range	499:507	its range of possible applications	499:532	The bacteriolytic enzyme lysozyme is widely used in medicine as an antimicrobial agent, and covalent immobilization of lysozyme can expand its range of possible applications.
30868059	2	33	theme	antimicrobial	427:439	arg1	agent					441:445	an antimicrobial agent	424:445	an antimicrobial agent	424:445	The bacteriolytic enzyme lysozyme is widely used in medicine as an antimicrobial agent, and covalent immobilization of lysozyme can expand its range of possible applications.
30868059	4	34	theme	immobilized	828:838	arg1	lysozyme					840:847	soluble and immobilized lysozyme	816:847	soluble and immobilized lysozyme	816:847	Here, we demonstrate the differential effects of glycine and charged (basic and acidic) amino acids on the enzymatic lysis of Gram-positive and Gram-negative bacteria by soluble and immobilized lysozyme.
30868059	11	35	from	activity	1894:1901	arg1	understanding					1844:1856	A greater understanding	1834:1856	A greater understanding of the effect of amino acids on the activity of native and immobilized lysozyme	1834:1936	A greater understanding of the effect of amino acids on the activity of native and immobilized lysozyme is important for both the development of new materials for medical purposes and elucidating the interaction of lysozyme with bacterial cells.
30868059	11	35	from	activity	1894:1901	arg1	important					1941:1949	important	1941:1949	important	1941:1949	A greater understanding of the effect of amino acids on the activity of native and immobilized lysozyme is important for both the development of new materials for medical purposes and elucidating the interaction of lysozyme with bacterial cells.
30868059	4	36	theme	enzymatic	753:761	arg1	lysis					763:767	the enzymatic lysis	749:767	the enzymatic lysis of Gram-positive and Gram-negative bacteria by soluble and immobilized lysozyme	749:847	Here, we demonstrate the differential effects of glycine and charged (basic and acidic) amino acids on the enzymatic lysis of Gram-positive and Gram-negative bacteria by soluble and immobilized lysozyme.
30868059	10	37	theme	immobilized	1697:1707	arg1	lysozyme					1709:1716	immobilized lysozyme	1697:1716	immobilized lysozyme	1697:1716	The Michaelis constant (Km) values of immobilized lysozyme were increased by 1.5-fold for E. coli cell lysis and 4.6-fold for M. luteus cell lysis compared to soluble enzyme.
30868059	1	38	theme	traditional	304:314	arg1	enzymes					351:357	bacteriolytic enzymes	337:357	bacteriolytic enzymes	337:357	The emergence of new antibiotic-resistant bacterial strains means it is increasingly important to find alternatives to traditional antibiotics, such as bacteriolytic enzymes.
30868059	1	38	theme	traditional	304:314	arg1	antibiotics					316:326	traditional antibiotics	304:326	traditional antibiotics	304:326	The emergence of new antibiotic-resistant bacterial strains means it is increasingly important to find alternatives to traditional antibiotics, such as bacteriolytic enzymes.
30868059	2	39	theme	covalent	452:459	arg1	immobilization					461:474	covalent immobilization	452:474	covalent immobilization of lysozyme	452:486	The bacteriolytic enzyme lysozyme is widely used in medicine as an antimicrobial agent, and covalent immobilization of lysozyme can expand its range of possible applications.
30868059	5	40	theme	Escherichia coli	972:987	arg1	cells					989:993	Gram-negative Escherichia coli cells	958:993	Gram-negative Escherichia coli cells	958:993	Glycine and basic amino acids (histidine, lysine, and arginine) significantly increase the rate of lysis of Gram-negative Escherichia coli cells in the presence of soluble lysozyme, but they do not substantially affect the rate of enzymatic lysis of Gram-positive Micrococcus luteus.
30868059	8	41	theme	lysis	1417:1421	arg1	kinetics					1423:1430	cell lysis kinetics	1412:1430	cell lysis kinetics at bacterial concentrations below 4 × 108 colony-forming units·mL-1	1412:1498	For immobilized lysozyme, the presence of an external diffusion mode on cell lysis kinetics at bacterial concentrations below 4 × 108 colony-forming units·mL-1 was shown.
30868059	0	42	theme	native	30:35	arg1	lysozyme					64:71	native and covalently immobilized lysozyme	30:71	native and covalently immobilized lysozyme	30:71	The bacteriolytic activity of native and covalently immobilized lysozyme against Gram-positive and Gram-negative bacteria is differentially affected by charged amino acids and glycine.
30868059	12	43	theme	charged	2215:2221	arg1	acids					2229:2233	charged amino acids	2215:2233	charged amino acids	2215:2233	Of particular interest is our finding that lysozyme activity against Gram-negative bacteria is enhanced in the presence of glycine and charged amino acids over a wide range of concentrations.
30868059	0	44	theme	lysozyme	64:71	arg1	activity					18:25	The bacteriolytic activity	0:25	The bacteriolytic activity of native and covalently immobilized lysozyme against Gram-positive and Gram-negative bacteria	0:120	The bacteriolytic activity of native and covalently immobilized lysozyme against Gram-positive and Gram-negative bacteria is differentially affected by charged amino acids and glycine.
30868059	7	45	theme	lysis	1307:1311	arg1	rate					1294:1297	the rate	1290:1297	the rate of cell lysis	1290:1311	When using immobilized lysozyme, the effects of amino acids on the rate of cell lysis are significantly reduced.
30868059	1	46	dep	strains	237:243	arg1	means					245:249	means	245:249	means	245:249	The emergence of new antibiotic-resistant bacterial strains means it is increasingly important to find alternatives to traditional antibiotics, such as bacteriolytic enzymes.
30868059	5	47	dep	acids	874:878	arg1	acids					874:878	basic amino acids	862:878	basic amino acids (histidine, lysine, and arginine)	862:912	Glycine and basic amino acids (histidine, lysine, and arginine) significantly increase the rate of lysis of Gram-negative Escherichia coli cells in the presence of soluble lysozyme, but they do not substantially affect the rate of enzymatic lysis of Gram-positive Micrococcus luteus.
30868059	5	47	dep	acids	874:878	arg1	lysine					892:897	lysine	892:897	lysine	892:897	Glycine and basic amino acids (histidine, lysine, and arginine) significantly increase the rate of lysis of Gram-negative Escherichia coli cells in the presence of soluble lysozyme, but they do not substantially affect the rate of enzymatic lysis of Gram-positive Micrococcus luteus.
30868059	5	47	dep	acids	874:878	arg1	arginine					904:911	arginine	904:911	arginine	904:911	Glycine and basic amino acids (histidine, lysine, and arginine) significantly increase the rate of lysis of Gram-negative Escherichia coli cells in the presence of soluble lysozyme, but they do not substantially affect the rate of enzymatic lysis of Gram-positive Micrococcus luteus.
30868059	5	47	dep	acids	874:878	arg1	histidine					881:889	histidine	881:889	histidine	881:889	Glycine and basic amino acids (histidine, lysine, and arginine) significantly increase the rate of lysis of Gram-negative Escherichia coli cells in the presence of soluble lysozyme, but they do not substantially affect the rate of enzymatic lysis of Gram-positive Micrococcus luteus.
30868059	10	48	dep	increased	1723:1731	arg1	compared					1806:1813	compared	1806:1813	compared to soluble enzyme	1806:1831	The Michaelis constant (Km) values of immobilized lysozyme were increased by 1.5-fold for E. coli cell lysis and 4.6-fold for M. luteus cell lysis compared to soluble enzyme.
30868059	2	49	theme	bacteriolytic	364:376	arg1	lysozyme					385:392	The bacteriolytic enzyme lysozyme	360:392	The bacteriolytic enzyme lysozyme	360:392	The bacteriolytic enzyme lysozyme is widely used in medicine as an antimicrobial agent, and covalent immobilization of lysozyme can expand its range of possible applications.
30868059	2	49	theme	bacteriolytic	364:376	arg1	range					503:507	its range	499:507	its range of possible applications	499:532	The bacteriolytic enzyme lysozyme is widely used in medicine as an antimicrobial agent, and covalent immobilization of lysozyme can expand its range of possible applications.
30868059	2	49	theme	bacteriolytic	364:376	arg1	agent					441:445	an antimicrobial agent	424:445	an antimicrobial agent	424:445	The bacteriolytic enzyme lysozyme is widely used in medicine as an antimicrobial agent, and covalent immobilization of lysozyme can expand its range of possible applications.
30868059	5	50	theme	soluble	1014:1020	arg1	lysozyme					1022:1029	soluble lysozyme	1014:1029	soluble lysozyme	1014:1029	Glycine and basic amino acids (histidine, lysine, and arginine) significantly increase the rate of lysis of Gram-negative Escherichia coli cells in the presence of soluble lysozyme, but they do not substantially affect the rate of enzymatic lysis of Gram-positive Micrococcus luteus.
30868059	8	51	theme	4 × 108 colony-forming	1466:1487	arg1	units·mL-1					1489:1498	4 × 108 colony-forming units·mL-1	1466:1498	4 × 108 colony-forming units·mL-1	1466:1498	For immobilized lysozyme, the presence of an external diffusion mode on cell lysis kinetics at bacterial concentrations below 4 × 108 colony-forming units·mL-1 was shown.
30868059	10	52	theme	cell	1795:1798	arg1	lysis					1800:1804	M. luteus cell lysis	1785:1804	M. luteus cell lysis	1785:1804	The Michaelis constant (Km) values of immobilized lysozyme were increased by 1.5-fold for E. coli cell lysis and 4.6-fold for M. luteus cell lysis compared to soluble enzyme.
30868059	12	53	theme	particular	2083:2092	arg1	interest					2094:2101	particular interest	2083:2101	particular interest	2083:2101	Of particular interest is our finding that lysozyme activity against Gram-negative bacteria is enhanced in the presence of glycine and charged amino acids over a wide range of concentrations.
30868059	7	54	theme	acids	1281:1285	arg1	effects					1264:1270	the effects	1260:1270	the effects of amino acids on the rate of cell lysis	1260:1311	When using immobilized lysozyme, the effects of amino acids on the rate of cell lysis are significantly reduced.
30868059	3	55	from	information	544:554	arg1	effect					563:568	the effect	559:568	the effect of such immobilized preparations on whole bacterial cells	559:626	However, information on the effect of such immobilized preparations on whole bacterial cells is quite limited.
30868059	3	56	theme	such	573:576	arg1	preparations					590:601	such immobilized preparations	573:601	such immobilized preparations	573:601	However, information on the effect of such immobilized preparations on whole bacterial cells is quite limited.
30868059	6	57	theme	M. luteus	1216:1224	arg1	lysis					1190:1194	enzymatic lysis	1180:1194	enzymatic lysis of both E. coli and M. luteus	1180:1224	Glutamate and aspartate significantly enhance enzymatic lysis of both E. coli and M. luteus.
30868059	11	58	theme	lysozyme	2049:2056	arg1	interaction					2034:2044	the interaction	2030:2044	the interaction of lysozyme with bacterial cells	2030:2077	A greater understanding of the effect of amino acids on the activity of native and immobilized lysozyme is important for both the development of new materials for medical purposes and elucidating the interaction of lysozyme with bacterial cells.
30868059	6	59	theme	E. coli	1204:1210	arg1	lysis					1190:1194	enzymatic lysis	1180:1194	enzymatic lysis of both E. coli and M. luteus	1180:1224	Glutamate and aspartate significantly enhance enzymatic lysis of both E. coli and M. luteus.
30868059	10	60	theme	constant	1673:1680	arg1	values					1687:1692	The Michaelis constant (Km) values	1659:1692	The Michaelis constant (Km) values of immobilized lysozyme	1659:1716	The Michaelis constant (Km) values of immobilized lysozyme were increased by 1.5-fold for E. coli cell lysis and 4.6-fold for M. luteus cell lysis compared to soluble enzyme.
30868059	3	61	theme	whole	606:610	arg1	cells					622:626	whole bacterial cells	606:626	whole bacterial cells	606:626	However, information on the effect of such immobilized preparations on whole bacterial cells is quite limited.
30868059	11	62	from	effect	1865:1870	arg1	activity					1894:1901	the activity	1890:1901	the activity of native and immobilized lysozyme	1890:1936	A greater understanding of the effect of amino acids on the activity of native and immobilized lysozyme is important for both the development of new materials for medical purposes and elucidating the interaction of lysozyme with bacterial cells.
30868059	11	63	theme	effect	1865:1870	arg1	understanding					1844:1856	A greater understanding	1834:1856	A greater understanding of the effect of amino acids on the activity of native and immobilized lysozyme	1834:1936	A greater understanding of the effect of amino acids on the activity of native and immobilized lysozyme is important for both the development of new materials for medical purposes and elucidating the interaction of lysozyme with bacterial cells.
30868059	11	63	theme	effect	1865:1870	arg1	important					1941:1949	important	1941:1949	important	1941:1949	A greater understanding of the effect of amino acids on the activity of native and immobilized lysozyme is important for both the development of new materials for medical purposes and elucidating the interaction of lysozyme with bacterial cells.
30868059	3	64	theme	preparations	590:601	arg1	effect					563:568	the effect	559:568	the effect of such immobilized preparations on whole bacterial cells	559:626	However, information on the effect of such immobilized preparations on whole bacterial cells is quite limited.
30868059	5	65	theme	basic	862:866	arg1	acids					874:878	basic amino acids	862:878	basic amino acids (histidine, lysine, and arginine)	862:912	Glycine and basic amino acids (histidine, lysine, and arginine) significantly increase the rate of lysis of Gram-negative Escherichia coli cells in the presence of soluble lysozyme, but they do not substantially affect the rate of enzymatic lysis of Gram-positive Micrococcus luteus.
30868059	5	65	theme	basic	862:866	arg1	lysine					892:897	lysine	892:897	lysine	892:897	Glycine and basic amino acids (histidine, lysine, and arginine) significantly increase the rate of lysis of Gram-negative Escherichia coli cells in the presence of soluble lysozyme, but they do not substantially affect the rate of enzymatic lysis of Gram-positive Micrococcus luteus.
30868059	5	65	theme	basic	862:866	arg1	arginine					904:911	arginine	904:911	arginine	904:911	Glycine and basic amino acids (histidine, lysine, and arginine) significantly increase the rate of lysis of Gram-negative Escherichia coli cells in the presence of soluble lysozyme, but they do not substantially affect the rate of enzymatic lysis of Gram-positive Micrococcus luteus.
30868059	5	65	theme	basic	862:866	arg1	histidine					881:889	histidine	881:889	histidine	881:889	Glycine and basic amino acids (histidine, lysine, and arginine) significantly increase the rate of lysis of Gram-negative Escherichia coli cells in the presence of soluble lysozyme, but they do not substantially affect the rate of enzymatic lysis of Gram-positive Micrococcus luteus.
30868059	6	66	theme	enzymatic	1180:1188	arg1	lysis					1190:1194	enzymatic lysis	1180:1194	enzymatic lysis of both E. coli and M. luteus	1180:1224	Glutamate and aspartate significantly enhance enzymatic lysis of both E. coli and M. luteus.
30868059	11	67	theme	native	1906:1911	arg1	lysozyme					1929:1936	native and immobilized lysozyme	1906:1936	lysozyme	1929:1936	A greater understanding of the effect of amino acids on the activity of native and immobilized lysozyme is important for both the development of new materials for medical purposes and elucidating the interaction of lysozyme with bacterial cells.
30868059	0	68	theme	amino	160:164	arg1	acids					166:170	charged amino acids	152:170	charged amino acids	152:170	The bacteriolytic activity of native and covalently immobilized lysozyme against Gram-positive and Gram-negative bacteria is differentially affected by charged amino acids and glycine.
30868059	5	69	theme	enzymatic	1081:1089	arg1	lysis					1091:1095	enzymatic lysis	1081:1095	enzymatic lysis of Gram-positive Micrococcus luteus	1081:1131	Glycine and basic amino acids (histidine, lysine, and arginine) significantly increase the rate of lysis of Gram-negative Escherichia coli cells in the presence of soluble lysozyme, but they do not substantially affect the rate of enzymatic lysis of Gram-positive Micrococcus luteus.
30868059	1	70	theme	strains	237:243	arg1	important					270:278	important	270:278	important	270:278	The emergence of new antibiotic-resistant bacterial strains means it is increasingly important to find alternatives to traditional antibiotics, such as bacteriolytic enzymes.
30868059	1	70	theme	strains	237:243	arg1	emergence					189:197	The emergence	185:197	The emergence of new antibiotic-resistant bacterial strains means it	185:252	The emergence of new antibiotic-resistant bacterial strains means it is increasingly important to find alternatives to traditional antibiotics, such as bacteriolytic enzymes.
30868059	4	71	theme	differential	671:682	arg1	effects					684:690	the differential effects	667:690	the differential effects of glycine and charged (basic and acidic) amino acids on the enzymatic lysis of Gram-positive and Gram-negative bacteria by soluble and immobilized lysozyme	667:847	Here, we demonstrate the differential effects of glycine and charged (basic and acidic) amino acids on the enzymatic lysis of Gram-positive and Gram-negative bacteria by soluble and immobilized lysozyme.
30868059	9	72	theme	pH	1533:1534	arg1	optimum					1536:1542	the pH optimum	1529:1542	the pH optimum of lysozyme activity after immobilization	1529:1584	The broadening of the pH optimum of lysozyme activity after immobilization has been demonstrated for both Gram-positive and Gram-negative bacteria.
30868059	12	73	theme	lysozyme	2123:2130	arg1	activity					2132:2139	lysozyme activity	2123:2139	lysozyme activity against Gram-negative bacteria	2123:2170	Of particular interest is our finding that lysozyme activity against Gram-negative bacteria is enhanced in the presence of glycine and charged amino acids over a wide range of concentrations.
30868059	11	74	theme	amino	1875:1879	arg1	acids					1881:1885	amino acids	1875:1885	amino acids	1875:1885	A greater understanding of the effect of amino acids on the activity of native and immobilized lysozyme is important for both the development of new materials for medical purposes and elucidating the interaction of lysozyme with bacterial cells.
30868059	4	75	theme	soluble	816:822	arg1	lysozyme					840:847	soluble and immobilized lysozyme	816:847	soluble and immobilized lysozyme	816:847	Here, we demonstrate the differential effects of glycine and charged (basic and acidic) amino acids on the enzymatic lysis of Gram-positive and Gram-negative bacteria by soluble and immobilized lysozyme.
30868059	7	76	from	effects	1264:1270	arg1	rate					1294:1297	the rate	1290:1297	the rate of cell lysis	1290:1311	When using immobilized lysozyme, the effects of amino acids on the rate of cell lysis are significantly reduced.
30868059	4	77	theme	acids	740:744	arg1	effects					684:690	the differential effects	667:690	the differential effects of glycine and charged (basic and acidic) amino acids on the enzymatic lysis of Gram-positive and Gram-negative bacteria by soluble and immobilized lysozyme	667:847	Here, we demonstrate the differential effects of glycine and charged (basic and acidic) amino acids on the enzymatic lysis of Gram-positive and Gram-negative bacteria by soluble and immobilized lysozyme.
30868059	10	78	theme	cell	1757:1760	arg1	lysis					1762:1766	E. coli cell lysis	1749:1766	E. coli cell lysis	1749:1766	The Michaelis constant (Km) values of immobilized lysozyme were increased by 1.5-fold for E. coli cell lysis and 4.6-fold for M. luteus cell lysis compared to soluble enzyme.
30868059	12	79	dep	glycine	2203:2209	arg1	presence					2191:2198	presence	2191:2198	presence	2191:2198	Of particular interest is our finding that lysozyme activity against Gram-negative bacteria is enhanced in the presence of glycine and charged amino acids over a wide range of concentrations.
30868059	12	79	dep	glycine	2203:2209	arg1	the					2187:2189	the	2187:2189	the	2187:2189	Of particular interest is our finding that lysozyme activity against Gram-negative bacteria is enhanced in the presence of glycine and charged amino acids over a wide range of concentrations.
30868059	1	80	theme	antibiotic-resistant	206:225	arg1	strains					237:243	new antibiotic-resistant bacterial strains	202:243	new antibiotic-resistant bacterial strains means it	202:252	The emergence of new antibiotic-resistant bacterial strains means it is increasingly important to find alternatives to traditional antibiotics, such as bacteriolytic enzymes.
30868059	9	81	theme	activity	1556:1563	arg1	optimum					1536:1542	the pH optimum	1529:1542	the pH optimum of lysozyme activity after immobilization	1529:1584	The broadening of the pH optimum of lysozyme activity after immobilization has been demonstrated for both Gram-positive and Gram-negative bacteria.
30868059	3	82	from	effect	563:568	arg1	cells					622:626	whole bacterial cells	606:626	whole bacterial cells	606:626	However, information on the effect of such immobilized preparations on whole bacterial cells is quite limited.
30868059	11	83	with	interaction	2034:2044	arg1	cells					2073:2077	bacterial cells	2063:2077	bacterial cells	2063:2077	A greater understanding of the effect of amino acids on the activity of native and immobilized lysozyme is important for both the development of new materials for medical purposes and elucidating the interaction of lysozyme with bacterial cells.
30868059	2	84	theme	possible	512:519	arg1	applications					521:532	possible applications	512:532	possible applications	512:532	The bacteriolytic enzyme lysozyme is widely used in medicine as an antimicrobial agent, and covalent immobilization of lysozyme can expand its range of possible applications.
30868059	11	85	theme	greater	1836:1842	arg1	understanding					1844:1856	A greater understanding	1834:1856	A greater understanding of the effect of amino acids on the activity of native and immobilized lysozyme	1834:1936	A greater understanding of the effect of amino acids on the activity of native and immobilized lysozyme is important for both the development of new materials for medical purposes and elucidating the interaction of lysozyme with bacterial cells.
30868059	11	85	theme	greater	1836:1842	arg1	important					1941:1949	important	1941:1949	important	1941:1949	A greater understanding of the effect of amino acids on the activity of native and immobilized lysozyme is important for both the development of new materials for medical purposes and elucidating the interaction of lysozyme with bacterial cells.
30868059	7	86	theme	immobilized	1238:1248	arg1	lysozyme					1250:1257	immobilized lysozyme	1238:1257	immobilized lysozyme	1238:1257	When using immobilized lysozyme, the effects of amino acids on the rate of cell lysis are significantly reduced.
30868059	5	87	theme	cells	989:993	arg1	lysis					949:953	lysis	949:953	lysis of Gram-negative Escherichia coli cells	949:993	Glycine and basic amino acids (histidine, lysine, and arginine) significantly increase the rate of lysis of Gram-negative Escherichia coli cells in the presence of soluble lysozyme, but they do not substantially affect the rate of enzymatic lysis of Gram-positive Micrococcus luteus.
30868059	2	88	used	used	404:407	arg2	range					503:507	its range	499:507	its range of possible applications	499:532	The bacteriolytic enzyme lysozyme is widely used in medicine as an antimicrobial agent, and covalent immobilization of lysozyme can expand its range of possible applications.
30868059	2	88	used	used	404:407	arg2	lysozyme					385:392	The bacteriolytic enzyme lysozyme	360:392	The bacteriolytic enzyme lysozyme	360:392	The bacteriolytic enzyme lysozyme is widely used in medicine as an antimicrobial agent, and covalent immobilization of lysozyme can expand its range of possible applications.
30868059	2	88	used	used	404:407	arg2	agent					441:445	an antimicrobial agent	424:445	an antimicrobial agent	424:445	The bacteriolytic enzyme lysozyme is widely used in medicine as an antimicrobial agent, and covalent immobilization of lysozyme can expand its range of possible applications.
30868059	4	89	theme	Gram-positive	772:784	arg1	bacteria					804:811	Gram-positive and Gram-negative bacteria	772:811	Gram-positive and Gram-negative bacteria	772:811	Here, we demonstrate the differential effects of glycine and charged (basic and acidic) amino acids on the enzymatic lysis of Gram-positive and Gram-negative bacteria by soluble and immobilized lysozyme.
30868059	5	90	theme	Micrococcus luteus	1114:1131	arg1	lysis					1091:1095	enzymatic lysis	1081:1095	enzymatic lysis of Gram-positive Micrococcus luteus	1081:1131	Glycine and basic amino acids (histidine, lysine, and arginine) significantly increase the rate of lysis of Gram-negative Escherichia coli cells in the presence of soluble lysozyme, but they do not substantially affect the rate of enzymatic lysis of Gram-positive Micrococcus luteus.
30868059	1	91	theme	bacterial	227:235	arg1	strains					237:243	new antibiotic-resistant bacterial strains	202:243	new antibiotic-resistant bacterial strains means it	202:252	The emergence of new antibiotic-resistant bacterial strains means it is increasingly important to find alternatives to traditional antibiotics, such as bacteriolytic enzymes.
30868059	4	92	theme	Gram-negative	790:802	arg1	bacteria					804:811	Gram-positive and Gram-negative bacteria	772:811	Gram-positive and Gram-negative bacteria	772:811	Here, we demonstrate the differential effects of glycine and charged (basic and acidic) amino acids on the enzymatic lysis of Gram-positive and Gram-negative bacteria by soluble and immobilized lysozyme.
30868059	10	93	theme	lysozyme	1709:1716	arg1	values					1687:1692	The Michaelis constant (Km) values	1659:1692	The Michaelis constant (Km) values of immobilized lysozyme	1659:1716	The Michaelis constant (Km) values of immobilized lysozyme were increased by 1.5-fold for E. coli cell lysis and 4.6-fold for M. luteus cell lysis compared to soluble enzyme.
30868059	0	94	theme	bacteriolytic	4:16	arg1	activity					18:25	The bacteriolytic activity	0:25	The bacteriolytic activity of native and covalently immobilized lysozyme against Gram-positive and Gram-negative bacteria	0:120	The bacteriolytic activity of native and covalently immobilized lysozyme against Gram-positive and Gram-negative bacteria is differentially affected by charged amino acids and glycine.
30868059	5	95	theme	lysis	949:953	arg1	rate					941:944	the rate	937:944	the rate of lysis of Gram-negative Escherichia coli cells	937:993	Glycine and basic amino acids (histidine, lysine, and arginine) significantly increase the rate of lysis of Gram-negative Escherichia coli cells in the presence of soluble lysozyme, but they do not substantially affect the rate of enzymatic lysis of Gram-positive Micrococcus luteus.
30868059	2	96	theme	lysozyme	479:486	arg1	immobilization					461:474	covalent immobilization	452:474	covalent immobilization of lysozyme	452:486	The bacteriolytic enzyme lysozyme is widely used in medicine as an antimicrobial agent, and covalent immobilization of lysozyme can expand its range of possible applications.
30868059	8	97	theme	external	1385:1392	arg1	mode					1404:1407	an external diffusion mode	1382:1407	an external diffusion mode	1382:1407	For immobilized lysozyme, the presence of an external diffusion mode on cell lysis kinetics at bacterial concentrations below 4 × 108 colony-forming units·mL-1 was shown.
30868059	4	98	dep	glycine	695:701	arg1	acidic					726:731	acidic	726:731	acidic	726:731	Here, we demonstrate the differential effects of glycine and charged (basic and acidic) amino acids on the enzymatic lysis of Gram-positive and Gram-negative bacteria by soluble and immobilized lysozyme.
30868059	4	98	dep	glycine	695:701	arg1	basic					716:720	basic	716:720	basic	716:720	Here, we demonstrate the differential effects of glycine and charged (basic and acidic) amino acids on the enzymatic lysis of Gram-positive and Gram-negative bacteria by soluble and immobilized lysozyme.
30868059	9	99	theme	Gram-negative	1635:1647	arg1	bacteria					1649:1656	both Gram-positive and Gram-negative bacteria	1612:1656	both Gram-positive and Gram-negative bacteria	1612:1656	The broadening of the pH optimum of lysozyme activity after immobilization has been demonstrated for both Gram-positive and Gram-negative bacteria.
30868059	8	100	theme	diffusion	1394:1402	arg1	mode					1404:1407	an external diffusion mode	1382:1407	an external diffusion mode	1382:1407	For immobilized lysozyme, the presence of an external diffusion mode on cell lysis kinetics at bacterial concentrations below 4 × 108 colony-forming units·mL-1 was shown.
30868059	8	101	theme	mode	1404:1407	arg1	presence					1370:1377	the presence	1366:1377	the presence of an external diffusion mode on cell lysis kinetics at bacterial concentrations below 4 × 108 colony-forming units·mL-1	1366:1498	For immobilized lysozyme, the presence of an external diffusion mode on cell lysis kinetics at bacterial concentrations below 4 × 108 colony-forming units·mL-1 was shown.
30868059	11	102	theme	materials	1983:1991	arg1	development					1964:1974	both the development	1955:1974	development	1964:1974	A greater understanding of the effect of amino acids on the activity of native and immobilized lysozyme is important for both the development of new materials for medical purposes and elucidating the interaction of lysozyme with bacterial cells.
30868059	11	103	theme	lysozyme	1929:1936	arg1	activity					1894:1901	the activity	1890:1901	the activity of native and immobilized lysozyme	1890:1936	A greater understanding of the effect of amino acids on the activity of native and immobilized lysozyme is important for both the development of new materials for medical purposes and elucidating the interaction of lysozyme with bacterial cells.
30868059	8	104	theme	cell	1412:1415	arg1	kinetics					1423:1430	cell lysis kinetics	1412:1430	cell lysis kinetics at bacterial concentrations below 4 × 108 colony-forming units·mL-1	1412:1498	For immobilized lysozyme, the presence of an external diffusion mode on cell lysis kinetics at bacterial concentrations below 4 × 108 colony-forming units·mL-1 was shown.
30868059	0	105	theme	immobilized	52:62	arg1	lysozyme					64:71	native and covalently immobilized lysozyme	30:71	native and covalently immobilized lysozyme	30:71	The bacteriolytic activity of native and covalently immobilized lysozyme against Gram-positive and Gram-negative bacteria is differentially affected by charged amino acids and glycine.
30868059	1	106	theme	bacteriolytic	337:349	arg1	enzymes					351:357	bacteriolytic enzymes	337:357	bacteriolytic enzymes	337:357	The emergence of new antibiotic-resistant bacterial strains means it is increasingly important to find alternatives to traditional antibiotics, such as bacteriolytic enzymes.
30868059	7	107	theme	cell	1302:1305	arg1	lysis					1307:1311	cell lysis	1302:1311	cell lysis	1302:1311	When using immobilized lysozyme, the effects of amino acids on the rate of cell lysis are significantly reduced.
30868059	12	108	theme	wide	2242:2245	arg1	range					2247:2251	a wide range	2240:2251	a wide range of concentrations	2240:2269	Of particular interest is our finding that lysozyme activity against Gram-negative bacteria is enhanced in the presence of glycine and charged amino acids over a wide range of concentrations.
30868059	2	109	theme	enzyme	378:383	arg1	lysozyme					385:392	The bacteriolytic enzyme lysozyme	360:392	The bacteriolytic enzyme lysozyme	360:392	The bacteriolytic enzyme lysozyme is widely used in medicine as an antimicrobial agent, and covalent immobilization of lysozyme can expand its range of possible applications.
30868059	2	109	theme	enzyme	378:383	arg1	range					503:507	its range	499:507	its range of possible applications	499:532	The bacteriolytic enzyme lysozyme is widely used in medicine as an antimicrobial agent, and covalent immobilization of lysozyme can expand its range of possible applications.
30868059	2	109	theme	enzyme	378:383	arg1	agent					441:445	an antimicrobial agent	424:445	an antimicrobial agent	424:445	The bacteriolytic enzyme lysozyme is widely used in medicine as an antimicrobial agent, and covalent immobilization of lysozyme can expand its range of possible applications.
30868059	11	110	dep	development	1964:1974	arg1	both					1955:1958	both	1955:1958	both	1955:1958	A greater understanding of the effect of amino acids on the activity of native and immobilized lysozyme is important for both the development of new materials for medical purposes and elucidating the interaction of lysozyme with bacterial cells.
30868059	8	111	theme	bacterial	1435:1443	arg1	concentrations					1445:1458	bacterial concentrations	1435:1458	bacterial concentrations below 4 × 108 colony-forming units·mL-1	1435:1498	For immobilized lysozyme, the presence of an external diffusion mode on cell lysis kinetics at bacterial concentrations below 4 × 108 colony-forming units·mL-1 was shown.
30868059	10	112	theme	E. coli	1749:1755	arg1	lysis					1762:1766	E. coli cell lysis	1749:1766	E. coli cell lysis	1749:1766	The Michaelis constant (Km) values of immobilized lysozyme were increased by 1.5-fold for E. coli cell lysis and 4.6-fold for M. luteus cell lysis compared to soluble enzyme.
30868059	8	113	from	presence	1370:1377	arg1	kinetics					1423:1430	cell lysis kinetics	1412:1430	cell lysis kinetics at bacterial concentrations below 4 × 108 colony-forming units·mL-1	1412:1498	For immobilized lysozyme, the presence of an external diffusion mode on cell lysis kinetics at bacterial concentrations below 4 × 108 colony-forming units·mL-1 was shown.
30868059	5	114	theme	lysozyme	1022:1029	arg1	presence					1002:1009	the presence	998:1009	the presence of soluble lysozyme	998:1029	Glycine and basic amino acids (histidine, lysine, and arginine) significantly increase the rate of lysis of Gram-negative Escherichia coli cells in the presence of soluble lysozyme, but they do not substantially affect the rate of enzymatic lysis of Gram-positive Micrococcus luteus.
30868059	12	115	theme	amino	2223:2227	arg1	acids					2229:2233	charged amino acids	2215:2233	charged amino acids	2215:2233	Of particular interest is our finding that lysozyme activity against Gram-negative bacteria is enhanced in the presence of glycine and charged amino acids over a wide range of concentrations.
30868059	10	116	theme	M. luteus	1785:1793	arg1	lysis					1800:1804	M. luteus cell lysis	1785:1804	M. luteus cell lysis	1785:1804	The Michaelis constant (Km) values of immobilized lysozyme were increased by 1.5-fold for E. coli cell lysis and 4.6-fold for M. luteus cell lysis compared to soluble enzyme.
30868059	12	117	theme	concentrations	2256:2269	arg1	range					2247:2251	a wide range	2240:2251	a wide range of concentrations	2240:2269	Of particular interest is our finding that lysozyme activity against Gram-negative bacteria is enhanced in the presence of glycine and charged amino acids over a wide range of concentrations.
35541611	0	0	theme	hydrothermal	74:85	arg1	liquefaction					87:98	the hydrothermal liquefaction	70:98	the hydrothermal liquefaction of algae species	70:115	The role of polysaccharides and proteins in bio-oil production during the hydrothermal liquefaction of algae species.
35541611	0	1	from	role	4:7	arg1	production					52:61	bio-oil production	44:61	bio-oil production during the hydrothermal liquefaction of algae species	44:115	The role of polysaccharides and proteins in bio-oil production during the hydrothermal liquefaction of algae species.
35541611	1	2	from	effects	145:151	arg1	process					249:255	the hydrothermal liquefaction (HTL) process	213:255	the hydrothermal liquefaction (HTL) process of algae	213:264	In order to understand the effects of the major algal components-carbohydrates and proteins on the hydrothermal liquefaction (HTL) process of algae, the HTL of polysaccharides or proteins with lipids was performed at 220, 260, 300 °C, respectively.
35541611	3	3	contain	has	553:555	arg1	presence					513:520	the presence	509:520	the presence of polysaccharides or proteins	509:551	Results show that the presence of polysaccharides or proteins has little effect on bio-oil yield but increased the HHV and significantly changed the boiling point distribution as compared with the HTL of lipids.
35541611	3	3	contain	has	553:555	arg2	effect					564:569	little effect	557:569	little effect	557:569	Results show that the presence of polysaccharides or proteins has little effect on bio-oil yield but increased the HHV and significantly changed the boiling point distribution as compared with the HTL of lipids.
35541611	5	4	theme	hydrolysis	931:940	arg1	product					942:948	the hydrolysis product	927:948	the hydrolysis product of polysaccharides/proteins	927:976	Heavy components in bio-oil were increased in the presence of polysaccharides or proteins, which was mainly caused by the hydrolysis product of polysaccharides/proteins being easily polymerized during the HTL process, forming macromolecular compounds into bio-oil.
35541611	5	4	theme	hydrolysis	931:940	arg1	polymerized					991:1001	polymerized	991:1001	polymerized	991:1001	Heavy components in bio-oil were increased in the presence of polysaccharides or proteins, which was mainly caused by the hydrolysis product of polysaccharides/proteins being easily polymerized during the HTL process, forming macromolecular compounds into bio-oil.
35541611	5	5	theme	polysaccharides/proteins	953:976	arg1	product					942:948	the hydrolysis product	927:948	the hydrolysis product of polysaccharides/proteins	927:976	Heavy components in bio-oil were increased in the presence of polysaccharides or proteins, which was mainly caused by the hydrolysis product of polysaccharides/proteins being easily polymerized during the HTL process, forming macromolecular compounds into bio-oil.
35541611	5	5	theme	polysaccharides/proteins	953:976	arg1	polymerized					991:1001	polymerized	991:1001	polymerized	991:1001	Heavy components in bio-oil were increased in the presence of polysaccharides or proteins, which was mainly caused by the hydrolysis product of polysaccharides/proteins being easily polymerized during the HTL process, forming macromolecular compounds into bio-oil.
35541611	4	6	theme	bio-oils	723:730	arg1	compositions					707:718	The compositions	703:718	The compositions of bio-oils from the HTL of binary mixtures	703:762	The compositions of bio-oils from the HTL of binary mixtures were similar to that from the HTL of lipids.
35541611	4	6	theme	bio-oils	723:730	arg1	similar					769:775	similar	769:775	similar	769:775	The compositions of bio-oils from the HTL of binary mixtures were similar to that from the HTL of lipids.
35541611	1	7	theme	algae	260:264	arg1	process					249:255	the hydrothermal liquefaction (HTL) process	213:255	the hydrothermal liquefaction (HTL) process of algae	213:264	In order to understand the effects of the major algal components-carbohydrates and proteins on the hydrothermal liquefaction (HTL) process of algae, the HTL of polysaccharides or proteins with lipids was performed at 220, 260, 300 °C, respectively.
35541611	2	8	theme	components	479:488	arg1	liquefaction					447:458	the individual liquefaction	432:458	the individual liquefaction of the major algal components	432:488	Bio-oil yields and qualities were investigated and compared with the individual liquefaction of the major algal components.
35541611	3	9	theme	lipids	695:700	arg1	HTL					688:690	the HTL	684:690	the HTL of lipids	684:700	Results show that the presence of polysaccharides or proteins has little effect on bio-oil yield but increased the HHV and significantly changed the boiling point distribution as compared with the HTL of lipids.
35541611	0	10	theme	algae	103:107	arg1	species					109:115	algae species	103:115	algae species	103:115	The role of polysaccharides and proteins in bio-oil production during the hydrothermal liquefaction of algae species.
35541611	2	11	theme	individual	436:445	arg1	liquefaction					447:458	the individual liquefaction	432:458	the individual liquefaction of the major algal components	432:488	Bio-oil yields and qualities were investigated and compared with the individual liquefaction of the major algal components.
35541611	1	12	with	HTL	271:273	arg1	lipids					311:316	lipids	311:316	lipids	311:316	In order to understand the effects of the major algal components-carbohydrates and proteins on the hydrothermal liquefaction (HTL) process of algae, the HTL of polysaccharides or proteins with lipids was performed at 220, 260, 300 °C, respectively.
35541611	1	13	theme	major	160:164	arg1	components-carbohydrates					172:195	the major algal components-carbohydrates	156:195	the major algal components-carbohydrates	156:195	In order to understand the effects of the major algal components-carbohydrates and proteins on the hydrothermal liquefaction (HTL) process of algae, the HTL of polysaccharides or proteins with lipids was performed at 220, 260, 300 °C, respectively.
35541611	4	14	theme	lipids	801:806	arg1	HTL					794:796	the HTL	790:796	the HTL of lipids	790:806	The compositions of bio-oils from the HTL of binary mixtures were similar to that from the HTL of lipids.
35541611	1	15	theme	algal	166:170	arg1	components-carbohydrates					172:195	the major algal components-carbohydrates	156:195	the major algal components-carbohydrates	156:195	In order to understand the effects of the major algal components-carbohydrates and proteins on the hydrothermal liquefaction (HTL) process of algae, the HTL of polysaccharides or proteins with lipids was performed at 220, 260, 300 °C, respectively.
35541611	3	16	theme	proteins	544:551	arg1	presence					513:520	the presence	509:520	the presence of polysaccharides or proteins	509:551	Results show that the presence of polysaccharides or proteins has little effect on bio-oil yield but increased the HHV and significantly changed the boiling point distribution as compared with the HTL of lipids.
35541611	1	17	theme	components-carbohydrates	172:195	arg1	effects					145:151	the effects	141:151	the effects of the major algal components-carbohydrates and proteins on the hydrothermal liquefaction (HTL) process of algae	141:264	In order to understand the effects of the major algal components-carbohydrates and proteins on the hydrothermal liquefaction (HTL) process of algae, the HTL of polysaccharides or proteins with lipids was performed at 220, 260, 300 °C, respectively.
35541611	0	18	theme	polysaccharides	12:26	arg1	role					4:7	The role	0:7	The role of polysaccharides and proteins in bio-oil production during the hydrothermal liquefaction of algae species.	0:116	The role of polysaccharides and proteins in bio-oil production during the hydrothermal liquefaction of algae species.
35541611	1	19	dep	understand	130:139	arg1	to					127:128	to	127:128	to	127:128	In order to understand the effects of the major algal components-carbohydrates and proteins on the hydrothermal liquefaction (HTL) process of algae, the HTL of polysaccharides or proteins with lipids was performed at 220, 260, 300 °C, respectively.
35541611	4	20	from	HTL	741:743	arg1	compositions					707:718	The compositions	703:718	The compositions of bio-oils from the HTL of binary mixtures	703:762	The compositions of bio-oils from the HTL of binary mixtures were similar to that from the HTL of lipids.
35541611	4	20	from	HTL	741:743	arg1	similar					769:775	similar	769:775	similar	769:775	The compositions of bio-oils from the HTL of binary mixtures were similar to that from the HTL of lipids.
35541611	5	21	theme	proteins	890:897	arg1	presence					859:866	the presence	855:866	the presence	855:866	Heavy components in bio-oil were increased in the presence of polysaccharides or proteins, which was mainly caused by the hydrolysis product of polysaccharides/proteins being easily polymerized during the HTL process, forming macromolecular compounds into bio-oil.
35541611	3	22	theme	polysaccharides	525:539	arg1	presence					513:520	the presence	509:520	the presence of polysaccharides or proteins	509:551	Results show that the presence of polysaccharides or proteins has little effect on bio-oil yield but increased the HHV and significantly changed the boiling point distribution as compared with the HTL of lipids.
35541611	1	23	theme	proteins	201:208	arg1	effects					145:151	the effects	141:151	the effects of the major algal components-carbohydrates and proteins on the hydrothermal liquefaction (HTL) process of algae	141:264	In order to understand the effects of the major algal components-carbohydrates and proteins on the hydrothermal liquefaction (HTL) process of algae, the HTL of polysaccharides or proteins with lipids was performed at 220, 260, 300 °C, respectively.
35541611	0	24	theme	proteins	32:39	arg1	role					4:7	The role	0:7	The role of polysaccharides and proteins in bio-oil production during the hydrothermal liquefaction of algae species.	0:116	The role of polysaccharides and proteins in bio-oil production during the hydrothermal liquefaction of algae species.
35541611	0	25	theme	species	109:115	arg1	liquefaction					87:98	the hydrothermal liquefaction	70:98	the hydrothermal liquefaction of algae species	70:115	The role of polysaccharides and proteins in bio-oil production during the hydrothermal liquefaction of algae species.
35541611	2	26	theme	Bio-oil	367:373	arg1	yields					375:380	Bio-oil yields	367:380	Bio-oil yields	367:380	Bio-oil yields and qualities were investigated and compared with the individual liquefaction of the major algal components.
35541611	1	27	theme	polysaccharides	278:292	arg1	HTL					271:273	the HTL	267:273	the HTL of polysaccharides or proteins with lipids	267:316	In order to understand the effects of the major algal components-carbohydrates and proteins on the hydrothermal liquefaction (HTL) process of algae, the HTL of polysaccharides or proteins with lipids was performed at 220, 260, 300 °C, respectively.
35541611	5	28	theme	macromolecular	1035:1048	arg1	compounds					1050:1058	macromolecular compounds	1035:1058	macromolecular compounds	1035:1058	Heavy components in bio-oil were increased in the presence of polysaccharides or proteins, which was mainly caused by the hydrolysis product of polysaccharides/proteins being easily polymerized during the HTL process, forming macromolecular compounds into bio-oil.
35541611	2	29	theme	algal	473:477	arg1	components					479:488	the major algal components	463:488	the major algal components	463:488	Bio-oil yields and qualities were investigated and compared with the individual liquefaction of the major algal components.
35541611	0	30	theme	bio-oil	44:50	arg1	production					52:61	bio-oil production	44:61	bio-oil production during the hydrothermal liquefaction of algae species	44:115	The role of polysaccharides and proteins in bio-oil production during the hydrothermal liquefaction of algae species.
35541611	1	31	theme	proteins	297:304	arg1	HTL					271:273	the HTL	267:273	the HTL of polysaccharides or proteins with lipids	267:316	In order to understand the effects of the major algal components-carbohydrates and proteins on the hydrothermal liquefaction (HTL) process of algae, the HTL of polysaccharides or proteins with lipids was performed at 220, 260, 300 °C, respectively.
35541611	3	32	theme	boiling	640:646	arg1	distribution					654:665	the boiling point distribution	636:665	the boiling point distribution	636:665	Results show that the presence of polysaccharides or proteins has little effect on bio-oil yield but increased the HHV and significantly changed the boiling point distribution as compared with the HTL of lipids.
35541611	3	33	theme	little	557:562	arg1	effect					564:569	little effect	557:569	little effect	557:569	Results show that the presence of polysaccharides or proteins has little effect on bio-oil yield but increased the HHV and significantly changed the boiling point distribution as compared with the HTL of lipids.
35541611	3	34	theme	point	648:652	arg1	distribution					654:665	the boiling point distribution	636:665	the boiling point distribution	636:665	Results show that the presence of polysaccharides or proteins has little effect on bio-oil yield but increased the HHV and significantly changed the boiling point distribution as compared with the HTL of lipids.
35541611	4	35	theme	binary	748:753	arg1	mixtures					755:762	binary mixtures	748:762	binary mixtures	748:762	The compositions of bio-oils from the HTL of binary mixtures were similar to that from the HTL of lipids.
35541611	1	36	theme	hydrothermal	217:228	arg1	process					249:255	the hydrothermal liquefaction (HTL) process	213:255	the hydrothermal liquefaction (HTL) process of algae	213:264	In order to understand the effects of the major algal components-carbohydrates and proteins on the hydrothermal liquefaction (HTL) process of algae, the HTL of polysaccharides or proteins with lipids was performed at 220, 260, 300 °C, respectively.
35541611	5	37	theme	HTL	1014:1016	arg1	process					1018:1024	the HTL process	1010:1024	the HTL process	1010:1024	Heavy components in bio-oil were increased in the presence of polysaccharides or proteins, which was mainly caused by the hydrolysis product of polysaccharides/proteins being easily polymerized during the HTL process, forming macromolecular compounds into bio-oil.
35541611	3	38	theme	increased	592:600	arg1	HHV					606:608	the HHV	602:608	increased the HHV	592:608	Results show that the presence of polysaccharides or proteins has little effect on bio-oil yield but increased the HHV and significantly changed the boiling point distribution as compared with the HTL of lipids.
35541611	5	39	from	components	815:824	arg1	bio-oil					829:835	bio-oil	829:835	bio-oil	829:835	Heavy components in bio-oil were increased in the presence of polysaccharides or proteins, which was mainly caused by the hydrolysis product of polysaccharides/proteins being easily polymerized during the HTL process, forming macromolecular compounds into bio-oil.
35541611	5	40	theme	polysaccharides	871:885	arg1	presence					859:866	the presence	855:866	the presence	855:866	Heavy components in bio-oil were increased in the presence of polysaccharides or proteins, which was mainly caused by the hydrolysis product of polysaccharides/proteins being easily polymerized during the HTL process, forming macromolecular compounds into bio-oil.
35541611	1	41	theme	liquefaction	230:241	arg1	process					249:255	the hydrothermal liquefaction (HTL) process	213:255	the hydrothermal liquefaction (HTL) process of algae	213:264	In order to understand the effects of the major algal components-carbohydrates and proteins on the hydrothermal liquefaction (HTL) process of algae, the HTL of polysaccharides or proteins with lipids was performed at 220, 260, 300 °C, respectively.
35541611	2	42	theme	major	467:471	arg1	components					479:488	the major algal components	463:488	the major algal components	463:488	Bio-oil yields and qualities were investigated and compared with the individual liquefaction of the major algal components.
35541611	3	43	theme	bio-oil	574:580	arg1	yield					582:586	bio-oil yield	574:586	bio-oil yield	574:586	Results show that the presence of polysaccharides or proteins has little effect on bio-oil yield but increased the HHV and significantly changed the boiling point distribution as compared with the HTL of lipids.
35541611	5	44	theme	Heavy	809:813	arg1	components					815:824	Heavy components	809:824	Heavy components in bio-oil	809:835	Heavy components in bio-oil were increased in the presence of polysaccharides or proteins, which was mainly caused by the hydrolysis product of polysaccharides/proteins being easily polymerized during the HTL process, forming macromolecular compounds into bio-oil.
35541611	4	45	theme	mixtures	755:762	arg1	HTL					741:743	the HTL	737:743	the HTL of binary mixtures	737:762	The compositions of bio-oils from the HTL of binary mixtures were similar to that from the HTL of lipids.
35541611	1	46	theme	HTL	244:246	arg1	process					249:255	the hydrothermal liquefaction (HTL) process	213:255	the hydrothermal liquefaction (HTL) process of algae	213:264	In order to understand the effects of the major algal components-carbohydrates and proteins on the hydrothermal liquefaction (HTL) process of algae, the HTL of polysaccharides or proteins with lipids was performed at 220, 260, 300 °C, respectively.
31370188	2	0	theme	diacyl	480:485	arg1	species					500:506	diacyl phospholipid species	480:506	diacyl phospholipid species	480:506	AA distributes differentially across membrane phospholipids, which is largely due to the action of coenzyme A-independent transacylase (CoA-IT), an enzyme that moves the fatty acid primarily from diacyl phospholipid species to ether-containing species, particularly the ethanolamine plasmalogens.
31370188	6	1	theme	phospholipase	1335:1347	arg1	A2β					1349:1351	calcium-independent phospholipase A2β	1315:1351	calcium-independent phospholipase A2β	1315:1351	Further, knockdown of cytosolic-group IVC phospholipase A2γ (cPLA2γ) by RNA silencing significantly reduced AA remodeling, while inhibition of other major phospholipase A2 forms such as cytosolic phospholipase A2α, calcium-independent phospholipase A2β, or secreted phospholipase A2 had no effect.
31370188	6	2	theme	cytosolic-group	1122:1136	arg1	cPLA2γ					1161:1166	cPLA2γ	1161:1166	cPLA2γ	1161:1166	Further, knockdown of cytosolic-group IVC phospholipase A2γ (cPLA2γ) by RNA silencing significantly reduced AA remodeling, while inhibition of other major phospholipase A2 forms such as cytosolic phospholipase A2α, calcium-independent phospholipase A2β, or secreted phospholipase A2 had no effect.
31370188	6	2	theme	cytosolic-group	1122:1136	arg1	A2γ					1156:1158	cytosolic-group IVC phospholipase A2γ	1122:1158	cytosolic-group IVC phospholipase A2γ (cPLA2γ)	1122:1167	Further, knockdown of cytosolic-group IVC phospholipase A2γ (cPLA2γ) by RNA silencing significantly reduced AA remodeling, while inhibition of other major phospholipase A2 forms such as cytosolic phospholipase A2α, calcium-independent phospholipase A2β, or secreted phospholipase A2 had no effect.
31370188	5	3	link	yeast-derived	965:977	arg1	zymosan					979:985	yeast-derived zymosan	965:985	yeast-derived zymosan	965:985	Cell stimulation with yeast-derived zymosan also had no effect on AA remodeling, but incubating the cells in AA-rich media markedly slowed down the process.
31370188	7	4	theme	unrecognized	1538:1549	arg1	role					1551:1554	a hitherto unrecognized role	1527:1554	a hitherto unrecognized role for cPLA2γ in maintaining membrane phospholipid composition via regulation of AA remodeling	1527:1646	These results uncover new regulatory features of CoA-IT-mediated transacylation reactions in cellular AA homeostasis and suggest a hitherto unrecognized role for cPLA2γ in maintaining membrane phospholipid composition via regulation of AA remodeling.
31370188	5	5	with	stimulation	948:958	arg1	zymosan					979:985	yeast-derived zymosan	965:985	yeast-derived zymosan	965:985	Cell stimulation with yeast-derived zymosan also had no effect on AA remodeling, but incubating the cells in AA-rich media markedly slowed down the process.
31370188	3	6	theme	plasmalogen	642:652	arg1	content					654:660	plasmalogen content	642:660	plasmalogen content	642:660	In this work, we examined the dependence of AA remodeling on plasmalogen content using the murine macrophage cell line RAW264.7 and its plasmalogen-deficient variants RAW.12 and RAW.108.
31370188	5	7	theme	incubating	1028:1037	arg1	cells					1043:1047	the cells	1039:1047	incubating the cells in AA-rich media	1028:1064	Cell stimulation with yeast-derived zymosan also had no effect on AA remodeling, but incubating the cells in AA-rich media markedly slowed down the process.
31370188	6	8	theme	phospholipase	1255:1267	arg1	A2					1269:1270	other major phospholipase A2	1243:1270	other major phospholipase A2 forms such as cytosolic phospholipase A2α, calcium-independent phospholipase A2β, or secreted phospholipase A2	1243:1381	Further, knockdown of cytosolic-group IVC phospholipase A2γ (cPLA2γ) by RNA silencing significantly reduced AA remodeling, while inhibition of other major phospholipase A2 forms such as cytosolic phospholipase A2α, calcium-independent phospholipase A2β, or secreted phospholipase A2 had no effect.
31370188	6	9	theme	other	1243:1247	arg1	A2					1269:1270	other major phospholipase A2	1243:1270	other major phospholipase A2 forms such as cytosolic phospholipase A2α, calcium-independent phospholipase A2β, or secreted phospholipase A2	1243:1381	Further, knockdown of cytosolic-group IVC phospholipase A2γ (cPLA2γ) by RNA silencing significantly reduced AA remodeling, while inhibition of other major phospholipase A2 forms such as cytosolic phospholipase A2α, calcium-independent phospholipase A2β, or secreted phospholipase A2 had no effect.
31370188	7	10	theme	CoA-IT-mediated	1447:1461	arg1	reactions					1478:1486	CoA-IT-mediated transacylation reactions	1447:1486	CoA-IT-mediated transacylation reactions in cellular AA homeostasis	1447:1513	These results uncover new regulatory features of CoA-IT-mediated transacylation reactions in cellular AA homeostasis and suggest a hitherto unrecognized role for cPLA2γ in maintaining membrane phospholipid composition via regulation of AA remodeling.
31370188	1	11	theme	cellular	253:260	arg1	synthesis					273:281	cellular eicosanoid synthesis	253:281	cellular eicosanoid synthesis	253:281	Availability of free arachidonic acid (AA) constitutes a rate limiting factor for cellular eicosanoid synthesis.
31370188	5	12	theme	AA	1009:1010	arg1	remodeling					1012:1021	AA remodeling	1009:1021	AA remodeling	1009:1021	Cell stimulation with yeast-derived zymosan also had no effect on AA remodeling, but incubating the cells in AA-rich media markedly slowed down the process.
31370188	3	13	from	dependence	611:620	arg1	content					654:660	plasmalogen content	642:660	plasmalogen content	642:660	In this work, we examined the dependence of AA remodeling on plasmalogen content using the murine macrophage cell line RAW264.7 and its plasmalogen-deficient variants RAW.12 and RAW.108.
31370188	1	14	theme	eicosanoid	262:271	arg1	synthesis					273:281	cellular eicosanoid synthesis	253:281	cellular eicosanoid synthesis	253:281	Availability of free arachidonic acid (AA) constitutes a rate limiting factor for cellular eicosanoid synthesis.
31370188	2	15	theme	ether-containing	511:526	arg1	plasmalogens					567:578	the ethanolamine plasmalogens	550:578	the ethanolamine plasmalogens	550:578	AA distributes differentially across membrane phospholipids, which is largely due to the action of coenzyme A-independent transacylase (CoA-IT), an enzyme that moves the fatty acid primarily from diacyl phospholipid species to ether-containing species, particularly the ethanolamine plasmalogens.
31370188	2	15	theme	ether-containing	511:526	arg1	species					528:534	ether-containing species	511:534	ether-containing species	511:534	AA distributes differentially across membrane phospholipids, which is largely due to the action of coenzyme A-independent transacylase (CoA-IT), an enzyme that moves the fatty acid primarily from diacyl phospholipid species to ether-containing species, particularly the ethanolamine plasmalogens.
31370188	6	16	theme	phospholipase	1296:1308	arg1	A2α					1310:1312	cytosolic phospholipase A2α	1286:1312	cytosolic phospholipase A2α	1286:1312	Further, knockdown of cytosolic-group IVC phospholipase A2γ (cPLA2γ) by RNA silencing significantly reduced AA remodeling, while inhibition of other major phospholipase A2 forms such as cytosolic phospholipase A2α, calcium-independent phospholipase A2β, or secreted phospholipase A2 had no effect.
31370188	6	17	theme	AA	1208:1209	arg1	remodeling					1211:1220	AA remodeling	1208:1220	AA remodeling	1208:1220	Further, knockdown of cytosolic-group IVC phospholipase A2γ (cPLA2γ) by RNA silencing significantly reduced AA remodeling, while inhibition of other major phospholipase A2 forms such as cytosolic phospholipase A2α, calcium-independent phospholipase A2β, or secreted phospholipase A2 had no effect.
31370188	1	18	theme	free	187:190	arg1	AA					210:211	AA	210:211	AA	210:211	Availability of free arachidonic acid (AA) constitutes a rate limiting factor for cellular eicosanoid synthesis.
31370188	1	18	theme	free	187:190	arg1	acid					204:207	free arachidonic acid	187:207	free arachidonic acid (AA)	187:212	Availability of free arachidonic acid (AA) constitutes a rate limiting factor for cellular eicosanoid synthesis.
31370188	2	19	theme	transacylase	406:417	arg1	CoA-IT					420:425	coenzyme A-independent transacylase (CoA-IT)	383:426	coenzyme A-independent transacylase (CoA-IT)	383:426	AA distributes differentially across membrane phospholipids, which is largely due to the action of coenzyme A-independent transacylase (CoA-IT), an enzyme that moves the fatty acid primarily from diacyl phospholipid species to ether-containing species, particularly the ethanolamine plasmalogens.
31370188	2	19	theme	transacylase	406:417	arg1	enzyme					432:437	an enzyme	429:437	an enzyme that moves the fatty acid primarily from diacyl phospholipid species to ether-containing species, particularly the ethanolamine plasmalogens	429:578	AA distributes differentially across membrane phospholipids, which is largely due to the action of coenzyme A-independent transacylase (CoA-IT), an enzyme that moves the fatty acid primarily from diacyl phospholipid species to ether-containing species, particularly the ethanolamine plasmalogens.
31370188	4	20	with	phospholipids	807:819	arg1	kinetics					848:855	kinetics	848:855	kinetics	848:855	All three strains remodeled AA between phospholipids with similar magnitude and kinetics, thus demonstrating that cellular plasmalogen content does not influence the process.
31370188	4	20	with	phospholipids	807:819	arg1	magnitude					834:842	similar magnitude	826:842	similar magnitude	826:842	All three strains remodeled AA between phospholipids with similar magnitude and kinetics, thus demonstrating that cellular plasmalogen content does not influence the process.
31370188	3	21	theme	plasmalogen-deficient	717:737	arg1	variants					739:746	its plasmalogen-deficient variants	713:746	its plasmalogen-deficient variants RAW.12 and RAW.108	713:765	In this work, we examined the dependence of AA remodeling on plasmalogen content using the murine macrophage cell line RAW264.7 and its plasmalogen-deficient variants RAW.12 and RAW.108.
31370188	3	21	theme	plasmalogen-deficient	717:737	arg1	RAW.108					759:765	RAW.108	759:765	RAW.108	759:765	In this work, we examined the dependence of AA remodeling on plasmalogen content using the murine macrophage cell line RAW264.7 and its plasmalogen-deficient variants RAW.12 and RAW.108.
31370188	3	21	theme	plasmalogen-deficient	717:737	arg1	RAW.12					748:753	RAW.12	748:753	RAW.12	748:753	In this work, we examined the dependence of AA remodeling on plasmalogen content using the murine macrophage cell line RAW264.7 and its plasmalogen-deficient variants RAW.12 and RAW.108.
31370188	6	22	theme	cytosolic	1286:1294	arg1	A2α					1310:1312	cytosolic phospholipase A2α	1286:1312	cytosolic phospholipase A2α	1286:1312	Further, knockdown of cytosolic-group IVC phospholipase A2γ (cPLA2γ) by RNA silencing significantly reduced AA remodeling, while inhibition of other major phospholipase A2 forms such as cytosolic phospholipase A2α, calcium-independent phospholipase A2β, or secreted phospholipase A2 had no effect.
31370188	7	23	theme	cellular	1491:1498	arg1	homeostasis					1503:1513	cellular AA homeostasis	1491:1513	cellular AA homeostasis	1491:1513	These results uncover new regulatory features of CoA-IT-mediated transacylation reactions in cellular AA homeostasis and suggest a hitherto unrecognized role for cPLA2γ in maintaining membrane phospholipid composition via regulation of AA remodeling.
31370188	2	24	theme	A-independent	392:404	arg1	CoA-IT					420:425	coenzyme A-independent transacylase (CoA-IT)	383:426	coenzyme A-independent transacylase (CoA-IT)	383:426	AA distributes differentially across membrane phospholipids, which is largely due to the action of coenzyme A-independent transacylase (CoA-IT), an enzyme that moves the fatty acid primarily from diacyl phospholipid species to ether-containing species, particularly the ethanolamine plasmalogens.
31370188	2	24	theme	A-independent	392:404	arg1	enzyme					432:437	an enzyme	429:437	an enzyme that moves the fatty acid primarily from diacyl phospholipid species to ether-containing species, particularly the ethanolamine plasmalogens	429:578	AA distributes differentially across membrane phospholipids, which is largely due to the action of coenzyme A-independent transacylase (CoA-IT), an enzyme that moves the fatty acid primarily from diacyl phospholipid species to ether-containing species, particularly the ethanolamine plasmalogens.
31370188	0	25	theme	Plasmalogen	9:19	arg1	Content					21:27	Cellular Plasmalogen Content	0:27	Cellular Plasmalogen Content	0:27	Cellular Plasmalogen Content Does Not Influence Arachidonic Acid Levels or Distribution in Macrophages: A Role for Cytosolic Phospholipase A2γ in Phospholipid Remodeling.
31370188	4	26	theme	plasmalogen	891:901	arg1	content					903:909	cellular plasmalogen content	882:909	cellular plasmalogen content	882:909	All three strains remodeled AA between phospholipids with similar magnitude and kinetics, thus demonstrating that cellular plasmalogen content does not influence the process.
31370188	7	27	theme	transacylation	1463:1476	arg1	reactions					1478:1486	CoA-IT-mediated transacylation reactions	1447:1486	CoA-IT-mediated transacylation reactions in cellular AA homeostasis	1447:1513	These results uncover new regulatory features of CoA-IT-mediated transacylation reactions in cellular AA homeostasis and suggest a hitherto unrecognized role for cPLA2γ in maintaining membrane phospholipid composition via regulation of AA remodeling.
31370188	2	28	theme	coenzyme	383:390	arg1	CoA-IT					420:425	coenzyme A-independent transacylase (CoA-IT)	383:426	coenzyme A-independent transacylase (CoA-IT)	383:426	AA distributes differentially across membrane phospholipids, which is largely due to the action of coenzyme A-independent transacylase (CoA-IT), an enzyme that moves the fatty acid primarily from diacyl phospholipid species to ether-containing species, particularly the ethanolamine plasmalogens.
31370188	2	28	theme	coenzyme	383:390	arg1	enzyme					432:437	an enzyme	429:437	an enzyme that moves the fatty acid primarily from diacyl phospholipid species to ether-containing species, particularly the ethanolamine plasmalogens	429:578	AA distributes differentially across membrane phospholipids, which is largely due to the action of coenzyme A-independent transacylase (CoA-IT), an enzyme that moves the fatty acid primarily from diacyl phospholipid species to ether-containing species, particularly the ethanolamine plasmalogens.
31370188	0	29	theme	Cellular	0:7	arg1	Content					21:27	Cellular Plasmalogen Content	0:27	Cellular Plasmalogen Content	0:27	Cellular Plasmalogen Content Does Not Influence Arachidonic Acid Levels or Distribution in Macrophages: A Role for Cytosolic Phospholipase A2γ in Phospholipid Remodeling.
31370188	3	30	dep	line	695:698	arg1	RAW264.7					700:707	RAW264.7	700:707	the murine macrophage cell line RAW264.7	668:707	In this work, we examined the dependence of AA remodeling on plasmalogen content using the murine macrophage cell line RAW264.7 and its plasmalogen-deficient variants RAW.12 and RAW.108.
31370188	4	31	theme	cellular	882:889	arg1	content					903:909	cellular plasmalogen content	882:909	cellular plasmalogen content	882:909	All three strains remodeled AA between phospholipids with similar magnitude and kinetics, thus demonstrating that cellular plasmalogen content does not influence the process.
31370188	2	32	theme	membrane	321:328	arg1	phospholipids					330:342	membrane phospholipids	321:342	membrane phospholipids	321:342	AA distributes differentially across membrane phospholipids, which is largely due to the action of coenzyme A-independent transacylase (CoA-IT), an enzyme that moves the fatty acid primarily from diacyl phospholipid species to ether-containing species, particularly the ethanolamine plasmalogens.
31370188	7	33	theme	membrane	1582:1589	arg1	composition					1604:1614	membrane phospholipid composition	1582:1614	membrane phospholipid composition	1582:1614	These results uncover new regulatory features of CoA-IT-mediated transacylation reactions in cellular AA homeostasis and suggest a hitherto unrecognized role for cPLA2γ in maintaining membrane phospholipid composition via regulation of AA remodeling.
31370188	0	34	from	Distribution	75:86	arg1	Macrophages					91:101	Macrophages	91:101	Macrophages	91:101	Cellular Plasmalogen Content Does Not Influence Arachidonic Acid Levels or Distribution in Macrophages: A Role for Cytosolic Phospholipase A2γ in Phospholipid Remodeling.
31370188	7	35	theme	reactions	1478:1486	arg1	features					1435:1442	new regulatory features	1420:1442	new regulatory features of CoA-IT-mediated transacylation reactions in cellular AA homeostasis	1420:1513	These results uncover new regulatory features of CoA-IT-mediated transacylation reactions in cellular AA homeostasis and suggest a hitherto unrecognized role for cPLA2γ in maintaining membrane phospholipid composition via regulation of AA remodeling.
31370188	0	36	theme	Cytosolic	115:123	arg1	A2γ					139:141	Cytosolic Phospholipase A2γ	115:141	Cytosolic Phospholipase A2γ	115:141	Cellular Plasmalogen Content Does Not Influence Arachidonic Acid Levels or Distribution in Macrophages: A Role for Cytosolic Phospholipase A2γ in Phospholipid Remodeling.
31370188	7	37	theme	new	1420:1422	arg1	features					1435:1442	new regulatory features	1420:1442	new regulatory features of CoA-IT-mediated transacylation reactions in cellular AA homeostasis	1420:1513	These results uncover new regulatory features of CoA-IT-mediated transacylation reactions in cellular AA homeostasis and suggest a hitherto unrecognized role for cPLA2γ in maintaining membrane phospholipid composition via regulation of AA remodeling.
31370188	6	38	theme	RNA	1172:1174	arg1	silencing					1176:1184	RNA silencing	1172:1184	RNA silencing	1172:1184	Further, knockdown of cytosolic-group IVC phospholipase A2γ (cPLA2γ) by RNA silencing significantly reduced AA remodeling, while inhibition of other major phospholipase A2 forms such as cytosolic phospholipase A2α, calcium-independent phospholipase A2β, or secreted phospholipase A2 had no effect.
31370188	7	39	theme	regulatory	1424:1433	arg1	features					1435:1442	new regulatory features	1420:1442	new regulatory features of CoA-IT-mediated transacylation reactions in cellular AA homeostasis	1420:1513	These results uncover new regulatory features of CoA-IT-mediated transacylation reactions in cellular AA homeostasis and suggest a hitherto unrecognized role for cPLA2γ in maintaining membrane phospholipid composition via regulation of AA remodeling.
31370188	1	40	theme	acid	204:207	arg1	Availability					171:182	Availability	171:182	Availability of free arachidonic acid (AA)	171:212	Availability of free arachidonic acid (AA) constitutes a rate limiting factor for cellular eicosanoid synthesis.
31370188	6	41	theme	A2γ	1156:1158	arg1	knockdown					1109:1117	knockdown	1109:1117	knockdown of cytosolic-group IVC phospholipase A2γ (cPLA2γ) by RNA silencing	1109:1184	Further, knockdown of cytosolic-group IVC phospholipase A2γ (cPLA2γ) by RNA silencing significantly reduced AA remodeling, while inhibition of other major phospholipase A2 forms such as cytosolic phospholipase A2α, calcium-independent phospholipase A2β, or secreted phospholipase A2 had no effect.
31370188	2	42	theme	ethanolamine	554:565	arg1	plasmalogens					567:578	the ethanolamine plasmalogens	550:578	the ethanolamine plasmalogens	550:578	AA distributes differentially across membrane phospholipids, which is largely due to the action of coenzyme A-independent transacylase (CoA-IT), an enzyme that moves the fatty acid primarily from diacyl phospholipid species to ether-containing species, particularly the ethanolamine plasmalogens.
31370188	2	42	theme	ethanolamine	554:565	arg1	species					528:534	ether-containing species	511:534	ether-containing species	511:534	AA distributes differentially across membrane phospholipids, which is largely due to the action of coenzyme A-independent transacylase (CoA-IT), an enzyme that moves the fatty acid primarily from diacyl phospholipid species to ether-containing species, particularly the ethanolamine plasmalogens.
31370188	5	43	theme	Cell	943:946	arg1	stimulation					948:958	Cell stimulation	943:958	Cell stimulation with yeast-derived zymosan	943:985	Cell stimulation with yeast-derived zymosan also had no effect on AA remodeling, but incubating the cells in AA-rich media markedly slowed down the process.
31370188	5	44	contain	had	992:994	arg2	effect					999:1004	no effect	996:1004	no effect	996:1004	Cell stimulation with yeast-derived zymosan also had no effect on AA remodeling, but incubating the cells in AA-rich media markedly slowed down the process.
31370188	5	44	contain	had	992:994	arg1	stimulation					948:958	Cell stimulation	943:958	Cell stimulation with yeast-derived zymosan	943:985	Cell stimulation with yeast-derived zymosan also had no effect on AA remodeling, but incubating the cells in AA-rich media markedly slowed down the process.
31370188	6	45	theme	secreted	1357:1364	arg1	A2					1380:1381	secreted phospholipase A2	1357:1381	secreted phospholipase A2	1357:1381	Further, knockdown of cytosolic-group IVC phospholipase A2γ (cPLA2γ) by RNA silencing significantly reduced AA remodeling, while inhibition of other major phospholipase A2 forms such as cytosolic phospholipase A2α, calcium-independent phospholipase A2β, or secreted phospholipase A2 had no effect.
31370188	5	46	from	cells	1043:1047	arg1	media					1060:1064	AA-rich media	1052:1064	AA-rich media	1052:1064	Cell stimulation with yeast-derived zymosan also had no effect on AA remodeling, but incubating the cells in AA-rich media markedly slowed down the process.
31370188	1	47	dep	limiting	233:240	arg1	rate					228:231	rate	228:231	rate	228:231	Availability of free arachidonic acid (AA) constitutes a rate limiting factor for cellular eicosanoid synthesis.
31370188	6	48	theme	forms	1272:1276	arg1	inhibition					1229:1238	inhibition	1229:1238	inhibition of other major phospholipase A2 forms such as cytosolic phospholipase A2α, calcium-independent phospholipase A2β, or secreted phospholipase A2	1229:1381	Further, knockdown of cytosolic-group IVC phospholipase A2γ (cPLA2γ) by RNA silencing significantly reduced AA remodeling, while inhibition of other major phospholipase A2 forms such as cytosolic phospholipase A2α, calcium-independent phospholipase A2β, or secreted phospholipase A2 had no effect.
31370188	1	49	theme	arachidonic	192:202	arg1	AA					210:211	AA	210:211	AA	210:211	Availability of free arachidonic acid (AA) constitutes a rate limiting factor for cellular eicosanoid synthesis.
31370188	1	49	theme	arachidonic	192:202	arg1	acid					204:207	free arachidonic acid	187:207	free arachidonic acid (AA)	187:212	Availability of free arachidonic acid (AA) constitutes a rate limiting factor for cellular eicosanoid synthesis.
31370188	2	50	theme	CoA-IT	420:425	arg1	action					373:378	the action	369:378	the action of coenzyme A-independent transacylase (CoA-IT), an enzyme that moves the fatty acid primarily from diacyl phospholipid species to ether-containing species, particularly the ethanolamine plasmalogens	369:578	AA distributes differentially across membrane phospholipids, which is largely due to the action of coenzyme A-independent transacylase (CoA-IT), an enzyme that moves the fatty acid primarily from diacyl phospholipid species to ether-containing species, particularly the ethanolamine plasmalogens.
31370188	0	51	theme	Phospholipase	125:137	arg1	A2γ					139:141	Cytosolic Phospholipase A2γ	115:141	Cytosolic Phospholipase A2γ	115:141	Cellular Plasmalogen Content Does Not Influence Arachidonic Acid Levels or Distribution in Macrophages: A Role for Cytosolic Phospholipase A2γ in Phospholipid Remodeling.
31370188	2	52	from	species	500:506	arg1	acid					460:463	the fatty acid	450:463	the fatty acid primarily from diacyl phospholipid species	450:506	AA distributes differentially across membrane phospholipids, which is largely due to the action of coenzyme A-independent transacylase (CoA-IT), an enzyme that moves the fatty acid primarily from diacyl phospholipid species to ether-containing species, particularly the ethanolamine plasmalogens.
31370188	5	53	theme	AA-rich	1052:1058	arg1	media					1060:1064	AA-rich media	1052:1064	AA-rich media	1052:1064	Cell stimulation with yeast-derived zymosan also had no effect on AA remodeling, but incubating the cells in AA-rich media markedly slowed down the process.
31370188	0	54	theme	Acid	60:63	arg1	Levels					65:70	Arachidonic Acid Levels	48:70	Arachidonic Acid Levels	48:70	Cellular Plasmalogen Content Does Not Influence Arachidonic Acid Levels or Distribution in Macrophages: A Role for Cytosolic Phospholipase A2γ in Phospholipid Remodeling.
31370188	3	55	dep	variants	739:746	arg1	variants					739:746	its plasmalogen-deficient variants	713:746	its plasmalogen-deficient variants RAW.12 and RAW.108	713:765	In this work, we examined the dependence of AA remodeling on plasmalogen content using the murine macrophage cell line RAW264.7 and its plasmalogen-deficient variants RAW.12 and RAW.108.
31370188	3	55	dep	variants	739:746	arg1	RAW.108					759:765	RAW.108	759:765	RAW.108	759:765	In this work, we examined the dependence of AA remodeling on plasmalogen content using the murine macrophage cell line RAW264.7 and its plasmalogen-deficient variants RAW.12 and RAW.108.
31370188	3	55	dep	variants	739:746	arg1	RAW.12					748:753	RAW.12	748:753	RAW.12	748:753	In this work, we examined the dependence of AA remodeling on plasmalogen content using the murine macrophage cell line RAW264.7 and its plasmalogen-deficient variants RAW.12 and RAW.108.
31370188	0	56	theme	Phospholipid	146:157	arg1	Remodeling					159:168	Phospholipid Remodeling	146:168	Phospholipid Remodeling	146:168	Cellular Plasmalogen Content Does Not Influence Arachidonic Acid Levels or Distribution in Macrophages: A Role for Cytosolic Phospholipase A2γ in Phospholipid Remodeling.
31370188	0	57	from	Levels	65:70	arg1	Macrophages					91:101	Macrophages	91:101	Macrophages	91:101	Cellular Plasmalogen Content Does Not Influence Arachidonic Acid Levels or Distribution in Macrophages: A Role for Cytosolic Phospholipase A2γ in Phospholipid Remodeling.
31370188	0	58	dep	Influence	38:46	arg1	Role					106:109	A Role	104:109	A Role for Cytosolic Phospholipase A2γ in Phospholipid Remodeling	104:168	Cellular Plasmalogen Content Does Not Influence Arachidonic Acid Levels or Distribution in Macrophages: A Role for Cytosolic Phospholipase A2γ in Phospholipid Remodeling.
31370188	7	59	theme	AA	1634:1635	arg1	remodeling					1637:1646	AA remodeling	1634:1646	AA remodeling	1634:1646	These results uncover new regulatory features of CoA-IT-mediated transacylation reactions in cellular AA homeostasis and suggest a hitherto unrecognized role for cPLA2γ in maintaining membrane phospholipid composition via regulation of AA remodeling.
31370188	0	60	theme	Arachidonic	48:58	arg1	Levels					65:70	Arachidonic Acid Levels	48:70	Arachidonic Acid Levels	48:70	Cellular Plasmalogen Content Does Not Influence Arachidonic Acid Levels or Distribution in Macrophages: A Role for Cytosolic Phospholipase A2γ in Phospholipid Remodeling.
31370188	0	61	from	Role	106:109	arg1	Remodeling					159:168	Phospholipid Remodeling	146:168	Phospholipid Remodeling	146:168	Cellular Plasmalogen Content Does Not Influence Arachidonic Acid Levels or Distribution in Macrophages: A Role for Cytosolic Phospholipase A2γ in Phospholipid Remodeling.
31370188	6	62	theme	major	1249:1253	arg1	A2					1269:1270	other major phospholipase A2	1243:1270	other major phospholipase A2 forms such as cytosolic phospholipase A2α, calcium-independent phospholipase A2β, or secreted phospholipase A2	1243:1381	Further, knockdown of cytosolic-group IVC phospholipase A2γ (cPLA2γ) by RNA silencing significantly reduced AA remodeling, while inhibition of other major phospholipase A2 forms such as cytosolic phospholipase A2α, calcium-independent phospholipase A2β, or secreted phospholipase A2 had no effect.
31370188	7	63	theme	AA	1500:1501	arg1	homeostasis					1503:1513	cellular AA homeostasis	1491:1513	cellular AA homeostasis	1491:1513	These results uncover new regulatory features of CoA-IT-mediated transacylation reactions in cellular AA homeostasis and suggest a hitherto unrecognized role for cPLA2γ in maintaining membrane phospholipid composition via regulation of AA remodeling.
31370188	6	64	theme	calcium-independent	1315:1333	arg1	A2β					1349:1351	calcium-independent phospholipase A2β	1315:1351	calcium-independent phospholipase A2β	1315:1351	Further, knockdown of cytosolic-group IVC phospholipase A2γ (cPLA2γ) by RNA silencing significantly reduced AA remodeling, while inhibition of other major phospholipase A2 forms such as cytosolic phospholipase A2α, calcium-independent phospholipase A2β, or secreted phospholipase A2 had no effect.
31370188	3	65	theme	cell	690:693	arg1	line					695:698	the murine macrophage cell line	668:698	the murine macrophage cell line RAW264.7	668:707	In this work, we examined the dependence of AA remodeling on plasmalogen content using the murine macrophage cell line RAW264.7 and its plasmalogen-deficient variants RAW.12 and RAW.108.
31370188	3	66	theme	AA	625:626	arg1	remodeling					628:637	AA remodeling	625:637	AA remodeling	625:637	In this work, we examined the dependence of AA remodeling on plasmalogen content using the murine macrophage cell line RAW264.7 and its plasmalogen-deficient variants RAW.12 and RAW.108.
31370188	6	67	theme	A2	1269:1270	arg1	A2					1380:1381	secreted phospholipase A2	1357:1381	secreted phospholipase A2	1357:1381	Further, knockdown of cytosolic-group IVC phospholipase A2γ (cPLA2γ) by RNA silencing significantly reduced AA remodeling, while inhibition of other major phospholipase A2 forms such as cytosolic phospholipase A2α, calcium-independent phospholipase A2β, or secreted phospholipase A2 had no effect.
31370188	6	67	theme	A2	1269:1270	arg1	A2β					1349:1351	calcium-independent phospholipase A2β	1315:1351	calcium-independent phospholipase A2β	1315:1351	Further, knockdown of cytosolic-group IVC phospholipase A2γ (cPLA2γ) by RNA silencing significantly reduced AA remodeling, while inhibition of other major phospholipase A2 forms such as cytosolic phospholipase A2α, calcium-independent phospholipase A2β, or secreted phospholipase A2 had no effect.
31370188	6	67	theme	A2	1269:1270	arg1	A2α					1310:1312	cytosolic phospholipase A2α	1286:1312	cytosolic phospholipase A2α	1286:1312	Further, knockdown of cytosolic-group IVC phospholipase A2γ (cPLA2γ) by RNA silencing significantly reduced AA remodeling, while inhibition of other major phospholipase A2 forms such as cytosolic phospholipase A2α, calcium-independent phospholipase A2β, or secreted phospholipase A2 had no effect.
31370188	6	67	theme	A2	1269:1270	arg1	forms					1272:1276	other major phospholipase A2 forms	1243:1276	other major phospholipase A2 forms such as cytosolic phospholipase A2α, calcium-independent phospholipase A2β, or secreted phospholipase A2	1243:1381	Further, knockdown of cytosolic-group IVC phospholipase A2γ (cPLA2γ) by RNA silencing significantly reduced AA remodeling, while inhibition of other major phospholipase A2 forms such as cytosolic phospholipase A2α, calcium-independent phospholipase A2β, or secreted phospholipase A2 had no effect.
31370188	5	68	theme	yeast-derived	965:977	arg1	zymosan					979:985	yeast-derived zymosan	965:985	yeast-derived zymosan	965:985	Cell stimulation with yeast-derived zymosan also had no effect on AA remodeling, but incubating the cells in AA-rich media markedly slowed down the process.
31370188	7	69	from	features	1435:1442	arg1	homeostasis					1503:1513	cellular AA homeostasis	1491:1513	cellular AA homeostasis	1491:1513	These results uncover new regulatory features of CoA-IT-mediated transacylation reactions in cellular AA homeostasis and suggest a hitherto unrecognized role for cPLA2γ in maintaining membrane phospholipid composition via regulation of AA remodeling.
31370188	6	70	theme	phospholipase	1366:1378	arg1	A2					1380:1381	secreted phospholipase A2	1357:1381	secreted phospholipase A2	1357:1381	Further, knockdown of cytosolic-group IVC phospholipase A2γ (cPLA2γ) by RNA silencing significantly reduced AA remodeling, while inhibition of other major phospholipase A2 forms such as cytosolic phospholipase A2α, calcium-independent phospholipase A2β, or secreted phospholipase A2 had no effect.
31370188	7	71	from	homeostasis	1503:1513	arg1	features					1435:1442	new regulatory features	1420:1442	new regulatory features of CoA-IT-mediated transacylation reactions in cellular AA homeostasis	1420:1513	These results uncover new regulatory features of CoA-IT-mediated transacylation reactions in cellular AA homeostasis and suggest a hitherto unrecognized role for cPLA2γ in maintaining membrane phospholipid composition via regulation of AA remodeling.
31370188	3	72	theme	remodeling	628:637	arg1	dependence					611:620	the dependence	607:620	the dependence of AA remodeling on plasmalogen content	607:660	In this work, we examined the dependence of AA remodeling on plasmalogen content using the murine macrophage cell line RAW264.7 and its plasmalogen-deficient variants RAW.12 and RAW.108.
31370188	2	73	theme	phospholipid	487:498	arg1	species					500:506	diacyl phospholipid species	480:506	diacyl phospholipid species	480:506	AA distributes differentially across membrane phospholipids, which is largely due to the action of coenzyme A-independent transacylase (CoA-IT), an enzyme that moves the fatty acid primarily from diacyl phospholipid species to ether-containing species, particularly the ethanolamine plasmalogens.
31370188	6	74	contain	had	1383:1385	arg2	effect					1390:1395	no effect	1387:1395	no effect	1387:1395	Further, knockdown of cytosolic-group IVC phospholipase A2γ (cPLA2γ) by RNA silencing significantly reduced AA remodeling, while inhibition of other major phospholipase A2 forms such as cytosolic phospholipase A2α, calcium-independent phospholipase A2β, or secreted phospholipase A2 had no effect.
31370188	6	74	contain	had	1383:1385	arg1	inhibition					1229:1238	inhibition	1229:1238	inhibition of other major phospholipase A2 forms such as cytosolic phospholipase A2α, calcium-independent phospholipase A2β, or secreted phospholipase A2	1229:1381	Further, knockdown of cytosolic-group IVC phospholipase A2γ (cPLA2γ) by RNA silencing significantly reduced AA remodeling, while inhibition of other major phospholipase A2 forms such as cytosolic phospholipase A2α, calcium-independent phospholipase A2β, or secreted phospholipase A2 had no effect.
31370188	3	75	theme	murine	672:677	arg1	line					695:698	the murine macrophage cell line	668:698	the murine macrophage cell line RAW264.7	668:707	In this work, we examined the dependence of AA remodeling on plasmalogen content using the murine macrophage cell line RAW264.7 and its plasmalogen-deficient variants RAW.12 and RAW.108.
31370188	7	76	theme	remodeling	1637:1646	arg1	regulation					1620:1629	regulation	1620:1629	regulation of AA remodeling	1620:1646	These results uncover new regulatory features of CoA-IT-mediated transacylation reactions in cellular AA homeostasis and suggest a hitherto unrecognized role for cPLA2γ in maintaining membrane phospholipid composition via regulation of AA remodeling.
31370188	6	77	theme	phospholipase	1142:1154	arg1	cPLA2γ					1161:1166	cPLA2γ	1161:1166	cPLA2γ	1161:1166	Further, knockdown of cytosolic-group IVC phospholipase A2γ (cPLA2γ) by RNA silencing significantly reduced AA remodeling, while inhibition of other major phospholipase A2 forms such as cytosolic phospholipase A2α, calcium-independent phospholipase A2β, or secreted phospholipase A2 had no effect.
31370188	6	77	theme	phospholipase	1142:1154	arg1	A2γ					1156:1158	cytosolic-group IVC phospholipase A2γ	1122:1158	cytosolic-group IVC phospholipase A2γ (cPLA2γ)	1122:1167	Further, knockdown of cytosolic-group IVC phospholipase A2γ (cPLA2γ) by RNA silencing significantly reduced AA remodeling, while inhibition of other major phospholipase A2 forms such as cytosolic phospholipase A2α, calcium-independent phospholipase A2β, or secreted phospholipase A2 had no effect.
31370188	7	78	from	reactions	1478:1486	arg1	homeostasis					1503:1513	cellular AA homeostasis	1491:1513	cellular AA homeostasis	1491:1513	These results uncover new regulatory features of CoA-IT-mediated transacylation reactions in cellular AA homeostasis and suggest a hitherto unrecognized role for cPLA2γ in maintaining membrane phospholipid composition via regulation of AA remodeling.
31370188	3	79	theme	macrophage	679:688	arg1	line					695:698	the murine macrophage cell line	668:698	the murine macrophage cell line RAW264.7	668:707	In this work, we examined the dependence of AA remodeling on plasmalogen content using the murine macrophage cell line RAW264.7 and its plasmalogen-deficient variants RAW.12 and RAW.108.
31370188	2	80	theme	fatty	454:458	arg1	acid					460:463	the fatty acid	450:463	the fatty acid primarily from diacyl phospholipid species	450:506	AA distributes differentially across membrane phospholipids, which is largely due to the action of coenzyme A-independent transacylase (CoA-IT), an enzyme that moves the fatty acid primarily from diacyl phospholipid species to ether-containing species, particularly the ethanolamine plasmalogens.
31370188	4	81	theme	similar	826:832	arg1	magnitude					834:842	similar magnitude	826:842	similar magnitude	826:842	All three strains remodeled AA between phospholipids with similar magnitude and kinetics, thus demonstrating that cellular plasmalogen content does not influence the process.
31370188	7	82	theme	phospholipid	1591:1602	arg1	composition					1604:1614	membrane phospholipid composition	1582:1614	membrane phospholipid composition	1582:1614	These results uncover new regulatory features of CoA-IT-mediated transacylation reactions in cellular AA homeostasis and suggest a hitherto unrecognized role for cPLA2γ in maintaining membrane phospholipid composition via regulation of AA remodeling.
31370188	6	83	theme	IVC	1138:1140	arg1	cPLA2γ					1161:1166	cPLA2γ	1161:1166	cPLA2γ	1161:1166	Further, knockdown of cytosolic-group IVC phospholipase A2γ (cPLA2γ) by RNA silencing significantly reduced AA remodeling, while inhibition of other major phospholipase A2 forms such as cytosolic phospholipase A2α, calcium-independent phospholipase A2β, or secreted phospholipase A2 had no effect.
31370188	6	83	theme	IVC	1138:1140	arg1	A2γ					1156:1158	cytosolic-group IVC phospholipase A2γ	1122:1158	cytosolic-group IVC phospholipase A2γ (cPLA2γ)	1122:1167	Further, knockdown of cytosolic-group IVC phospholipase A2γ (cPLA2γ) by RNA silencing significantly reduced AA remodeling, while inhibition of other major phospholipase A2 forms such as cytosolic phospholipase A2α, calcium-independent phospholipase A2β, or secreted phospholipase A2 had no effect.
31370188	1	84	theme	limiting	233:240	arg1	factor					242:247	a rate limiting factor	226:247	a rate limiting factor for cellular eicosanoid synthesis	226:281	Availability of free arachidonic acid (AA) constitutes a rate limiting factor for cellular eicosanoid synthesis.
30053875	6	0	theme	suspension	862:871	arg1	array					873:877	a bead-based suspension array	849:877	a bead-based suspension array	849:877	RESULTS To enable detection of serum antibodies against Salmonella in pigs, a bead-based suspension array was developed with five LPS variants (B, 2× C1, C2, D1), each conjugated to a different bead set using triazine chemistry.
30053875	6	1	theme	triazine	982:989	arg1	chemistry					991:999	triazine chemistry	982:999	triazine chemistry	982:999	RESULTS To enable detection of serum antibodies against Salmonella in pigs, a bead-based suspension array was developed with five LPS variants (B, 2× C1, C2, D1), each conjugated to a different bead set using triazine chemistry.
30053875	14	2	theme	multiple	2247:2254	arg1	serovars					2267:2274	multiple Salmonella serovars	2247:2274	multiple Salmonella serovars	2247:2274	Ultimately, this type of assay can be applied in routine veterinary serology to test for antibodies against multiple Salmonella serovars (or other pathogens) in one single serum sample, using up-to-date antigen panels.
30053875	7	3	theme	beads	1020:1024	arg1	Reactivity					1002:1011	Reactivity	1002:1011	Reactivity of the beads	1002:1024	Reactivity of the beads was confirmed with rabbit agglutination sera and with experimental pig sera.
30053875	2	4	theme	cell	333:336	arg1	envelope					338:345	the Salmonella cell envelope	318:345	the Salmonella cell envelope	318:345	ELISAs used to investigate pig herds are based on the detection of antibodies against components of the Salmonella cell envelope.
30053875	12	5	from	serology	1821:1828	arg1	pigs					1833:1836	pigs	1833:1836	pigs	1833:1836	CONCLUSIONS A suspension array for Salmonella serology in pigs was developed, that detects more seropositive sera than ELISA, which is achieved by expanding the panel of Salmonella LPS variants, including C2 LPS.
30053875	10	6	theme	commercial	1547:1556	arg1	BC1D1-ELISA					1558:1568	a commercial BC1D1-ELISA	1545:1568	a commercial BC1D1-ELISA	1545:1568	Results of the suspension array (BC1C1C2D) suggest that more pigs are seroconverted than indicated by a commercial BC1D1-ELISA, and that most of these extra seropositive samples give a signal on one of the beads with C LPS.
30053875	12	7	theme	Salmonella	1810:1819	arg1	serology					1821:1828	Salmonella serology	1810:1828	Salmonella serology in pigs	1810:1836	CONCLUSIONS A suspension array for Salmonella serology in pigs was developed, that detects more seropositive sera than ELISA, which is achieved by expanding the panel of Salmonella LPS variants, including C2 LPS.
30053875	8	8	theme	sets	1126:1129	arg1	mixture					1110:1116	a mixture	1108:1116	a mixture of bead sets	1108:1129	With a mixture of bead sets, 175 sera from slaughter pigs were investigated for the presence of antibodies against Salmonella.
30053875	3	9	theme	lipopolysaccharide	540:557	arg1	composition					519:529	the composition	515:529	the composition of their lipopolysaccharide (LPS)	515:563	Nearly all Salmonella isolates in food-producing animals are serovars of Salmonella enterica subspecies enterica, distributed over various serogroups as determined by the composition of their lipopolysaccharide (LPS).
30053875	1	10	theme	pathogen	159:166	arg1	presence					134:141	the presence	130:141	the presence of the zoonotic pathogen Salmonella	130:177	BACKGROUND Slaughter pigs are monitored for the presence of the zoonotic pathogen Salmonella, using both serology and bacteriology.
30053875	4	11	theme	serogroup	662:670	arg1	D					672:672	serogroup D	662:672	serogroup D	662:672	ELISAs for Salmonella serology are usually based on serogroup B and C1 LPS, often combined with serogroup D or E LPS.
30053875	10	12	theme	seropositive	1600:1611	arg1	samples					1613:1619	these extra seropositive samples	1588:1619	these extra seropositive samples	1588:1619	Results of the suspension array (BC1C1C2D) suggest that more pigs are seroconverted than indicated by a commercial BC1D1-ELISA, and that most of these extra seropositive samples give a signal on one of the beads with C LPS.
30053875	13	13	theme	set	2113:2115	arg1	cut-offs					2117:2124	set cut-offs	2113:2124	set cut-offs per antigen	2113:2136	The results demonstrate that bead-based suspension arrays allow for testing of pig sera, with the advantage of being able to set cut-offs per antigen.
30053875	6	14	theme	LPS	903:905	arg1	variants					907:914	five LPS variants	898:914	five LPS variants (B, 2× C1, C2, D1)	898:933	RESULTS To enable detection of serum antibodies against Salmonella in pigs, a bead-based suspension array was developed with five LPS variants (B, 2× C1, C2, D1), each conjugated to a different bead set using triazine chemistry.
30053875	6	14	theme	LPS	903:905	arg1	each					936:939	each	936:939	each	936:939	RESULTS To enable detection of serum antibodies against Salmonella in pigs, a bead-based suspension array was developed with five LPS variants (B, 2× C1, C2, D1), each conjugated to a different bead set using triazine chemistry.
30053875	10	15	theme	suspension	1458:1467	arg1	BC1C1C2D					1476:1483	BC1C1C2D	1476:1483	BC1C1C2D	1476:1483	Results of the suspension array (BC1C1C2D) suggest that more pigs are seroconverted than indicated by a commercial BC1D1-ELISA, and that most of these extra seropositive samples give a signal on one of the beads with C LPS.
30053875	10	15	theme	suspension	1458:1467	arg1	array					1469:1473	the suspension array	1454:1473	the suspension array (BC1C1C2D)	1454:1484	Results of the suspension array (BC1C1C2D) suggest that more pigs are seroconverted than indicated by a commercial BC1D1-ELISA, and that most of these extra seropositive samples give a signal on one of the beads with C LPS.
30053875	11	16	theme	antibody	1755:1762	arg1	detection					1764:1772	antibody detection	1755:1772	antibody detection	1755:1772	These results show that expansion of a standard panel with more C LPS variants improves antibody detection.
30053875	9	17	theme	C	1331:1331	arg1	data					1325:1328	historic data	1316:1328	historic data (C LPS)	1316:1336	With a combination of ROC analysis (B and D LPS) and a prevalence estimation based on historic data (C LPS), individual cut-offs were defined for each LPS-conjugated bead set, and assay performance was evaluated.
30053875	9	17	theme	C	1331:1331	arg1	LPS					1333:1335	C LPS	1331:1335	C LPS	1331:1335	With a combination of ROC analysis (B and D LPS) and a prevalence estimation based on historic data (C LPS), individual cut-offs were defined for each LPS-conjugated bead set, and assay performance was evaluated.
30053875	9	18	dep	B	1266:1266	arg1	LPS					1274:1276	LPS	1274:1276	LPS	1274:1276	With a combination of ROC analysis (B and D LPS) and a prevalence estimation based on historic data (C LPS), individual cut-offs were defined for each LPS-conjugated bead set, and assay performance was evaluated.
30053875	12	19	theme	seropositive	1871:1882	arg1	sera					1884:1887	more seropositive sera	1866:1887	more seropositive sera	1866:1887	CONCLUSIONS A suspension array for Salmonella serology in pigs was developed, that detects more seropositive sera than ELISA, which is achieved by expanding the panel of Salmonella LPS variants, including C2 LPS.
30053875	4	20	dep	B	628:628	arg1	LPS					637:639	LPS	637:639	LPS	637:639	ELISAs for Salmonella serology are usually based on serogroup B and C1 LPS, often combined with serogroup D or E LPS.
30053875	13	21	theme	pig	2067:2069	arg1	sera					2071:2074	pig sera	2067:2074	pig sera	2067:2074	The results demonstrate that bead-based suspension arrays allow for testing of pig sera, with the advantage of being able to set cut-offs per antigen.
30053875	9	22	theme	assay	1410:1414	arg1	performance					1416:1426	assay performance	1410:1426	assay performance	1410:1426	With a combination of ROC analysis (B and D LPS) and a prevalence estimation based on historic data (C LPS), individual cut-offs were defined for each LPS-conjugated bead set, and assay performance was evaluated.
30053875	5	23	from	use	722:724	arg1	ELISA					747:751	a broad ELISA	739:751	a broad ELISA	739:751	Although C2 LPS may improve serology, use of C2 LPS in a broad ELISA was never achieved.
30053875	2	24	theme	pig	245:247	arg1	herds					249:253	pig herds	245:253	pig herds	245:253	ELISAs used to investigate pig herds are based on the detection of antibodies against components of the Salmonella cell envelope.
30053875	8	25	from	pigs	1156:1159	arg1	sera					1136:1139	175 sera	1132:1139	175 sera from slaughter pigs	1132:1159	With a mixture of bead sets, 175 sera from slaughter pigs were investigated for the presence of antibodies against Salmonella.
30053875	9	26	theme	individual	1339:1348	arg1	cut-offs					1350:1357	individual cut-offs	1339:1357	individual cut-offs	1339:1357	With a combination of ROC analysis (B and D LPS) and a prevalence estimation based on historic data (C LPS), individual cut-offs were defined for each LPS-conjugated bead set, and assay performance was evaluated.
30053875	14	27	theme	antigen	2342:2348	arg1	panels					2350:2355	up-to-date antigen panels	2331:2355	up-to-date antigen panels	2331:2355	Ultimately, this type of assay can be applied in routine veterinary serology to test for antibodies against multiple Salmonella serovars (or other pathogens) in one single serum sample, using up-to-date antigen panels.
30053875	0	28	theme	Salmonella	51:60	arg1	antibodies					62:71	Salmonella antibodies	51:71	Salmonella antibodies	51:71	A bead-based suspension array for the detection of Salmonella antibodies in pig sera.
30053875	5	29	theme	LPS	732:734	arg1	use					722:724	use	722:724	use of C2 LPS in a broad ELISA	722:751	Although C2 LPS may improve serology, use of C2 LPS in a broad ELISA was never achieved.
30053875	9	30	theme	LPS-conjugated	1381:1394	arg1	set					1401:1403	each LPS-conjugated bead set	1376:1403	each LPS-conjugated bead set	1376:1403	With a combination of ROC analysis (B and D LPS) and a prevalence estimation based on historic data (C LPS), individual cut-offs were defined for each LPS-conjugated bead set, and assay performance was evaluated.
30053875	14	31	theme	single	2304:2309	arg1	sample					2317:2322	one single serum sample	2300:2322	one single serum sample	2300:2322	Ultimately, this type of assay can be applied in routine veterinary serology to test for antibodies against multiple Salmonella serovars (or other pathogens) in one single serum sample, using up-to-date antigen panels.
30053875	0	32	from	detection	38:46	arg1	sera					80:83	pig sera	76:83	pig sera	76:83	A bead-based suspension array for the detection of Salmonella antibodies in pig sera.
30053875	7	33	theme	pig	1093:1095	arg1	sera					1097:1100	experimental pig sera	1080:1100	experimental pig sera	1080:1100	Reactivity of the beads was confirmed with rabbit agglutination sera and with experimental pig sera.
30053875	3	34	from	isolates	370:377	arg1	animals					397:403	food-producing animals	382:403	food-producing animals	382:403	Nearly all Salmonella isolates in food-producing animals are serovars of Salmonella enterica subspecies enterica, distributed over various serogroups as determined by the composition of their lipopolysaccharide (LPS).
30053875	6	35	theme	bead	967:970	arg1	set					972:974	a different bead set	955:974	a different bead set	955:974	RESULTS To enable detection of serum antibodies against Salmonella in pigs, a bead-based suspension array was developed with five LPS variants (B, 2× C1, C2, D1), each conjugated to a different bead set using triazine chemistry.
30053875	11	36	theme	more	1726:1729	arg1	variants					1737:1744	more C LPS variants	1726:1744	more C LPS variants	1726:1744	These results show that expansion of a standard panel with more C LPS variants improves antibody detection.
30053875	0	37	theme	suspension	13:22	arg1	array					24:28	A bead-based suspension array	0:28	A bead-based suspension array	0:28	A bead-based suspension array for the detection of Salmonella antibodies in pig sera.
30053875	13	38	theme	bead-based	2017:2026	arg1	arrays					2039:2044	bead-based suspension arrays	2017:2044	bead-based suspension arrays	2017:2044	The results demonstrate that bead-based suspension arrays allow for testing of pig sera, with the advantage of being able to set cut-offs per antigen.
30053875	1	39	theme	Slaughter	97:105	arg1	pigs					107:110	BACKGROUND Slaughter pigs	86:110	BACKGROUND Slaughter pigs	86:110	BACKGROUND Slaughter pigs are monitored for the presence of the zoonotic pathogen Salmonella, using both serology and bacteriology.
30053875	3	40	theme	enterica	452:459	arg1	isolates					370:377	Nearly all Salmonella isolates	348:377	Nearly all Salmonella isolates in food-producing animals	348:403	Nearly all Salmonella isolates in food-producing animals are serovars of Salmonella enterica subspecies enterica, distributed over various serogroups as determined by the composition of their lipopolysaccharide (LPS).
30053875	3	40	theme	enterica	452:459	arg1	serovars					409:416	serovars	409:416	serovars	409:416	Nearly all Salmonella isolates in food-producing animals are serovars of Salmonella enterica subspecies enterica, distributed over various serogroups as determined by the composition of their lipopolysaccharide (LPS).
30053875	11	41	theme	panel	1715:1719	arg1	expansion					1691:1699	expansion	1691:1699	expansion of a standard panel with more C LPS variants	1691:1744	These results show that expansion of a standard panel with more C LPS variants improves antibody detection.
30053875	9	42	theme	prevalence	1285:1294	arg1	estimation					1296:1305	a prevalence estimation	1283:1305	a prevalence estimation based on historic data (C LPS)	1283:1336	With a combination of ROC analysis (B and D LPS) and a prevalence estimation based on historic data (C LPS), individual cut-offs were defined for each LPS-conjugated bead set, and assay performance was evaluated.
30053875	7	43	theme	rabbit	1045:1050	arg1	sera					1066:1069	rabbit agglutination sera	1045:1069	rabbit agglutination sera	1045:1069	Reactivity of the beads was confirmed with rabbit agglutination sera and with experimental pig sera.
30053875	6	44	theme	serum	804:808	arg1	antibodies					810:819	serum antibodies	804:819	serum antibodies against Salmonella in pigs	804:846	RESULTS To enable detection of serum antibodies against Salmonella in pigs, a bead-based suspension array was developed with five LPS variants (B, 2× C1, C2, D1), each conjugated to a different bead set using triazine chemistry.
30053875	3	45	theme	food-producing	382:395	arg1	animals					397:403	food-producing animals	382:403	food-producing animals	382:403	Nearly all Salmonella isolates in food-producing animals are serovars of Salmonella enterica subspecies enterica, distributed over various serogroups as determined by the composition of their lipopolysaccharide (LPS).
30053875	12	46	theme	C2	1980:1981	arg1	LPS					1983:1985	C2 LPS	1980:1985	C2 LPS	1980:1985	CONCLUSIONS A suspension array for Salmonella serology in pigs was developed, that detects more seropositive sera than ELISA, which is achieved by expanding the panel of Salmonella LPS variants, including C2 LPS.
30053875	14	47	theme	routine	2188:2194	arg1	serology					2207:2214	routine veterinary serology	2188:2214	routine veterinary serology	2188:2214	Ultimately, this type of assay can be applied in routine veterinary serology to test for antibodies against multiple Salmonella serovars (or other pathogens) in one single serum sample, using up-to-date antigen panels.
30053875	2	48	theme	Salmonella	322:331	arg1	envelope					338:345	the Salmonella cell envelope	318:345	the Salmonella cell envelope	318:345	ELISAs used to investigate pig herds are based on the detection of antibodies against components of the Salmonella cell envelope.
30053875	9	49	theme	ROC	1252:1254	arg1	analysis					1256:1263	ROC analysis	1252:1263	ROC analysis (B and D LPS)	1252:1277	With a combination of ROC analysis (B and D LPS) and a prevalence estimation based on historic data (C LPS), individual cut-offs were defined for each LPS-conjugated bead set, and assay performance was evaluated.
30053875	14	50	theme	other	2280:2284	arg1	pathogens					2286:2294	other pathogens	2280:2294	other pathogens	2280:2294	Ultimately, this type of assay can be applied in routine veterinary serology to test for antibodies against multiple Salmonella serovars (or other pathogens) in one single serum sample, using up-to-date antigen panels.
30053875	3	51	theme	various	479:485	arg1	serogroups					487:496	various serogroups	479:496	various serogroups as determined by the composition of their lipopolysaccharide (LPS)	479:563	Nearly all Salmonella isolates in food-producing animals are serovars of Salmonella enterica subspecies enterica, distributed over various serogroups as determined by the composition of their lipopolysaccharide (LPS).
30053875	12	52	theme	LPS	1956:1958	arg1	LPS					1983:1985	C2 LPS	1980:1985	C2 LPS	1980:1985	CONCLUSIONS A suspension array for Salmonella serology in pigs was developed, that detects more seropositive sera than ELISA, which is achieved by expanding the panel of Salmonella LPS variants, including C2 LPS.
30053875	12	52	theme	LPS	1956:1958	arg1	variants					1960:1967	Salmonella LPS variants	1945:1967	Salmonella LPS variants	1945:1967	CONCLUSIONS A suspension array for Salmonella serology in pigs was developed, that detects more seropositive sera than ELISA, which is achieved by expanding the panel of Salmonella LPS variants, including C2 LPS.
30053875	9	53	theme	estimation	1296:1305	arg1	combination					1237:1247	a combination	1235:1247	a combination of ROC analysis (B and D LPS) and a prevalence estimation based on historic data (C LPS)	1235:1336	With a combination of ROC analysis (B and D LPS) and a prevalence estimation based on historic data (C LPS), individual cut-offs were defined for each LPS-conjugated bead set, and assay performance was evaluated.
30053875	14	54	theme	Salmonella	2256:2265	arg1	serovars					2267:2274	multiple Salmonella serovars	2247:2274	multiple Salmonella serovars	2247:2274	Ultimately, this type of assay can be applied in routine veterinary serology to test for antibodies against multiple Salmonella serovars (or other pathogens) in one single serum sample, using up-to-date antigen panels.
30053875	4	55	theme	serogroup	618:626	arg1	B					628:628	serogroup B	618:628	serogroup B	618:628	ELISAs for Salmonella serology are usually based on serogroup B and C1 LPS, often combined with serogroup D or E LPS.
30053875	5	56	theme	C2	729:730	arg1	LPS					732:734	C2 LPS	729:734	C2 LPS	729:734	Although C2 LPS may improve serology, use of C2 LPS in a broad ELISA was never achieved.
30053875	2	57	theme	envelope	338:345	arg1	components					304:313	components	304:313	components of the Salmonella cell envelope	304:345	ELISAs used to investigate pig herds are based on the detection of antibodies against components of the Salmonella cell envelope.
30053875	6	58	theme	bead-based	851:860	arg1	array					873:877	a bead-based suspension array	849:877	a bead-based suspension array	849:877	RESULTS To enable detection of serum antibodies against Salmonella in pigs, a bead-based suspension array was developed with five LPS variants (B, 2× C1, C2, D1), each conjugated to a different bead set using triazine chemistry.
30053875	4	59	theme	Salmonella	577:586	arg1	serology					588:595	Salmonella serology	577:595	Salmonella serology	577:595	ELISAs for Salmonella serology are usually based on serogroup B and C1 LPS, often combined with serogroup D or E LPS.
30053875	12	60	theme	suspension	1789:1798	arg1	array					1800:1804	A suspension array	1787:1804	CONCLUSIONS A suspension array for Salmonella serology in pigs	1775:1836	CONCLUSIONS A suspension array for Salmonella serology in pigs was developed, that detects more seropositive sera than ELISA, which is achieved by expanding the panel of Salmonella LPS variants, including C2 LPS.
30053875	8	61	theme	175	1132:1134	arg1	sera					1136:1139	175 sera	1132:1139	175 sera from slaughter pigs	1132:1159	With a mixture of bead sets, 175 sera from slaughter pigs were investigated for the presence of antibodies against Salmonella.
30053875	1	62	theme	zoonotic	150:157	arg1	pathogen					159:166	the zoonotic pathogen Salmonella	146:177	the zoonotic pathogen Salmonella	146:177	BACKGROUND Slaughter pigs are monitored for the presence of the zoonotic pathogen Salmonella, using both serology and bacteriology.
30053875	10	63	theme	more	1499:1502	arg1	pigs					1504:1507	more pigs	1499:1507	more pigs	1499:1507	Results of the suspension array (BC1C1C2D) suggest that more pigs are seroconverted than indicated by a commercial BC1D1-ELISA, and that most of these extra seropositive samples give a signal on one of the beads with C LPS.
30053875	8	64	theme	bead	1121:1124	arg1	sets					1126:1129	bead sets	1121:1129	bead sets	1121:1129	With a mixture of bead sets, 175 sera from slaughter pigs were investigated for the presence of antibodies against Salmonella.
30053875	9	65	dep	analysis	1256:1263	arg1	D					1272:1272	D	1272:1272	D	1272:1272	With a combination of ROC analysis (B and D LPS) and a prevalence estimation based on historic data (C LPS), individual cut-offs were defined for each LPS-conjugated bead set, and assay performance was evaluated.
30053875	9	65	dep	analysis	1256:1263	arg1	B					1266:1266	B	1266:1266	B	1266:1266	With a combination of ROC analysis (B and D LPS) and a prevalence estimation based on historic data (C LPS), individual cut-offs were defined for each LPS-conjugated bead set, and assay performance was evaluated.
30053875	10	66	theme	extra	1594:1598	arg1	samples					1613:1619	these extra seropositive samples	1588:1619	these extra seropositive samples	1588:1619	Results of the suspension array (BC1C1C2D) suggest that more pigs are seroconverted than indicated by a commercial BC1D1-ELISA, and that most of these extra seropositive samples give a signal on one of the beads with C LPS.
30053875	9	67	theme	historic	1316:1323	arg1	data					1325:1328	historic data	1316:1328	historic data (C LPS)	1316:1336	With a combination of ROC analysis (B and D LPS) and a prevalence estimation based on historic data (C LPS), individual cut-offs were defined for each LPS-conjugated bead set, and assay performance was evaluated.
30053875	9	67	theme	historic	1316:1323	arg1	LPS					1333:1335	C LPS	1331:1335	C LPS	1331:1335	With a combination of ROC analysis (B and D LPS) and a prevalence estimation based on historic data (C LPS), individual cut-offs were defined for each LPS-conjugated bead set, and assay performance was evaluated.
30053875	2	68	theme	antibodies	285:294	arg1	detection					272:280	the detection	268:280	the detection of antibodies against components of the Salmonella cell envelope	268:345	ELISAs used to investigate pig herds are based on the detection of antibodies against components of the Salmonella cell envelope.
30053875	8	69	theme	antibodies	1199:1208	arg1	presence					1187:1194	the presence	1183:1194	the presence of antibodies against Salmonella	1183:1227	With a mixture of bead sets, 175 sera from slaughter pigs were investigated for the presence of antibodies against Salmonella.
30053875	10	70	theme	array	1469:1473	arg1	Results					1443:1449	Results	1443:1449	Results of the suspension array (BC1C1C2D)	1443:1484	Results of the suspension array (BC1C1C2D) suggest that more pigs are seroconverted than indicated by a commercial BC1D1-ELISA, and that most of these extra seropositive samples give a signal on one of the beads with C LPS.
30053875	5	71	theme	C2	693:694	arg1	LPS					696:698	C2 LPS	693:698	C2 LPS	693:698	Although C2 LPS may improve serology, use of C2 LPS in a broad ELISA was never achieved.
30053875	8	72	theme	slaughter	1146:1154	arg1	pigs					1156:1159	slaughter pigs	1146:1159	slaughter pigs	1146:1159	With a mixture of bead sets, 175 sera from slaughter pigs were investigated for the presence of antibodies against Salmonella.
30053875	11	73	with	expansion	1691:1699	arg1	variants					1737:1744	more C LPS variants	1726:1744	more C LPS variants	1726:1744	These results show that expansion of a standard panel with more C LPS variants improves antibody detection.
30053875	11	74	theme	LPS	1733:1735	arg1	variants					1737:1744	more C LPS variants	1726:1744	more C LPS variants	1726:1744	These results show that expansion of a standard panel with more C LPS variants improves antibody detection.
30053875	10	75	with	beads	1649:1653	arg1	LPS					1662:1664	C LPS	1660:1664	C LPS	1660:1664	Results of the suspension array (BC1C1C2D) suggest that more pigs are seroconverted than indicated by a commercial BC1D1-ELISA, and that most of these extra seropositive samples give a signal on one of the beads with C LPS.
30053875	6	76	dep	variants	907:914	arg1	C1					923:924	2× C1	920:924	2× C1	920:924	RESULTS To enable detection of serum antibodies against Salmonella in pigs, a bead-based suspension array was developed with five LPS variants (B, 2× C1, C2, D1), each conjugated to a different bead set using triazine chemistry.
30053875	6	76	dep	variants	907:914	arg1	D1					931:932	D1	931:932	D1	931:932	RESULTS To enable detection of serum antibodies against Salmonella in pigs, a bead-based suspension array was developed with five LPS variants (B, 2× C1, C2, D1), each conjugated to a different bead set using triazine chemistry.
30053875	6	76	dep	variants	907:914	arg1	C2					927:928	C2	927:928	C2	927:928	RESULTS To enable detection of serum antibodies against Salmonella in pigs, a bead-based suspension array was developed with five LPS variants (B, 2× C1, C2, D1), each conjugated to a different bead set using triazine chemistry.
30053875	6	76	dep	variants	907:914	arg1	B					917:917	B	917:917	B	917:917	RESULTS To enable detection of serum antibodies against Salmonella in pigs, a bead-based suspension array was developed with five LPS variants (B, 2× C1, C2, D1), each conjugated to a different bead set using triazine chemistry.
30053875	10	77	theme	beads	1649:1653	arg1	one					1638:1640	one	1638:1640	one	1638:1640	Results of the suspension array (BC1C1C2D) suggest that more pigs are seroconverted than indicated by a commercial BC1D1-ELISA, and that most of these extra seropositive samples give a signal on one of the beads with C LPS.
30053875	10	77	theme	beads	1649:1653	arg1	beads					1649:1653	the beads	1645:1653	the beads with C LPS	1645:1664	Results of the suspension array (BC1C1C2D) suggest that more pigs are seroconverted than indicated by a commercial BC1D1-ELISA, and that most of these extra seropositive samples give a signal on one of the beads with C LPS.
30053875	13	78	theme	sera	2071:2074	arg1	testing					2056:2062	testing	2056:2062	testing of pig sera	2056:2074	The results demonstrate that bead-based suspension arrays allow for testing of pig sera, with the advantage of being able to set cut-offs per antigen.
30053875	0	79	theme	antibodies	62:71	arg1	detection					38:46	the detection	34:46	the detection of Salmonella antibodies in pig sera	34:83	A bead-based suspension array for the detection of Salmonella antibodies in pig sera.
30053875	4	80	dep	based	609:613	arg1	combined					648:655	combined	648:655	combined with serogroup D or E LPS	648:681	ELISAs for Salmonella serology are usually based on serogroup B and C1 LPS, often combined with serogroup D or E LPS.
30053875	14	81	theme	up-to-date	2331:2340	arg1	panels					2350:2355	up-to-date antigen panels	2331:2355	up-to-date antigen panels	2331:2355	Ultimately, this type of assay can be applied in routine veterinary serology to test for antibodies against multiple Salmonella serovars (or other pathogens) in one single serum sample, using up-to-date antigen panels.
30053875	14	82	theme	serum	2311:2315	arg1	sample					2317:2322	one single serum sample	2300:2322	one single serum sample	2300:2322	Ultimately, this type of assay can be applied in routine veterinary serology to test for antibodies against multiple Salmonella serovars (or other pathogens) in one single serum sample, using up-to-date antigen panels.
30053875	0	83	theme	pig	76:78	arg1	sera					80:83	pig sera	76:83	pig sera	76:83	A bead-based suspension array for the detection of Salmonella antibodies in pig sera.
30053875	4	84	theme	E	677:677	arg1	LPS					679:681	E LPS	677:681	E LPS	677:681	ELISAs for Salmonella serology are usually based on serogroup B and C1 LPS, often combined with serogroup D or E LPS.
30053875	6	85	theme	2×	920:921	arg1	C1					923:924	2× C1	920:924	2× C1	920:924	RESULTS To enable detection of serum antibodies against Salmonella in pigs, a bead-based suspension array was developed with five LPS variants (B, 2× C1, C2, D1), each conjugated to a different bead set using triazine chemistry.
30053875	6	85	theme	2×	920:921	arg1	B					917:917	B	917:917	B	917:917	RESULTS To enable detection of serum antibodies against Salmonella in pigs, a bead-based suspension array was developed with five LPS variants (B, 2× C1, C2, D1), each conjugated to a different bead set using triazine chemistry.
30053875	3	86	dep	serogroups	487:496	arg1	determined					501:510	determined	501:510	determined by the composition of their lipopolysaccharide (LPS)	501:563	Nearly all Salmonella isolates in food-producing animals are serovars of Salmonella enterica subspecies enterica, distributed over various serogroups as determined by the composition of their lipopolysaccharide (LPS).
30053875	7	87	theme	experimental	1080:1091	arg1	sera					1097:1100	experimental pig sera	1080:1100	experimental pig sera	1080:1100	Reactivity of the beads was confirmed with rabbit agglutination sera and with experimental pig sera.
30053875	9	88	theme	bead	1396:1399	arg1	set					1401:1403	each LPS-conjugated bead set	1376:1403	each LPS-conjugated bead set	1376:1403	With a combination of ROC analysis (B and D LPS) and a prevalence estimation based on historic data (C LPS), individual cut-offs were defined for each LPS-conjugated bead set, and assay performance was evaluated.
30053875	12	89	dep	CONCLUSIONS	1775:1785	arg1	array					1800:1804	A suspension array	1787:1804	CONCLUSIONS A suspension array for Salmonella serology in pigs	1775:1836	CONCLUSIONS A suspension array for Salmonella serology in pigs was developed, that detects more seropositive sera than ELISA, which is achieved by expanding the panel of Salmonella LPS variants, including C2 LPS.
30053875	11	90	theme	standard	1706:1713	arg1	panel					1715:1719	a standard panel	1704:1719	a standard panel	1704:1719	These results show that expansion of a standard panel with more C LPS variants improves antibody detection.
30053875	0	91	theme	bead-based	2:11	arg1	array					24:28	A bead-based suspension array	0:28	A bead-based suspension array	0:28	A bead-based suspension array for the detection of Salmonella antibodies in pig sera.
30053875	10	92	from	signal	1628:1633	arg1	one					1638:1640	one	1638:1640	one	1638:1640	Results of the suspension array (BC1C1C2D) suggest that more pigs are seroconverted than indicated by a commercial BC1D1-ELISA, and that most of these extra seropositive samples give a signal on one of the beads with C LPS.
30053875	10	92	from	signal	1628:1633	arg1	beads					1649:1653	the beads	1645:1653	the beads with C LPS	1645:1664	Results of the suspension array (BC1C1C2D) suggest that more pigs are seroconverted than indicated by a commercial BC1D1-ELISA, and that most of these extra seropositive samples give a signal on one of the beads with C LPS.
30053875	6	93	theme	different	957:965	arg1	set					972:974	a different bead set	955:974	a different bead set	955:974	RESULTS To enable detection of serum antibodies against Salmonella in pigs, a bead-based suspension array was developed with five LPS variants (B, 2× C1, C2, D1), each conjugated to a different bead set using triazine chemistry.
30053875	7	94	theme	agglutination	1052:1064	arg1	sera					1066:1069	rabbit agglutination sera	1045:1069	rabbit agglutination sera	1045:1069	Reactivity of the beads was confirmed with rabbit agglutination sera and with experimental pig sera.
30053875	1	95	theme	BACKGROUND	86:95	arg1	pigs					107:110	BACKGROUND Slaughter pigs	86:110	BACKGROUND Slaughter pigs	86:110	BACKGROUND Slaughter pigs are monitored for the presence of the zoonotic pathogen Salmonella, using both serology and bacteriology.
30053875	3	96	theme	Salmonella	359:368	arg1	isolates					370:377	Nearly all Salmonella isolates	348:377	Nearly all Salmonella isolates in food-producing animals	348:403	Nearly all Salmonella isolates in food-producing animals are serovars of Salmonella enterica subspecies enterica, distributed over various serogroups as determined by the composition of their lipopolysaccharide (LPS).
30053875	3	96	theme	Salmonella	359:368	arg1	serovars					409:416	serovars	409:416	serovars	409:416	Nearly all Salmonella isolates in food-producing animals are serovars of Salmonella enterica subspecies enterica, distributed over various serogroups as determined by the composition of their lipopolysaccharide (LPS).
30053875	11	97	theme	C	1731:1731	arg1	variants					1737:1744	more C LPS variants	1726:1744	more C LPS variants	1726:1744	These results show that expansion of a standard panel with more C LPS variants improves antibody detection.
30053875	13	98	theme	suspension	2028:2037	arg1	arrays					2039:2044	bead-based suspension arrays	2017:2044	bead-based suspension arrays	2017:2044	The results demonstrate that bead-based suspension arrays allow for testing of pig sera, with the advantage of being able to set cut-offs per antigen.
30053875	6	99	theme	antibodies	810:819	arg1	detection					791:799	detection	791:799	detection of serum antibodies against Salmonella in pigs	791:846	RESULTS To enable detection of serum antibodies against Salmonella in pigs, a bead-based suspension array was developed with five LPS variants (B, 2× C1, C2, D1), each conjugated to a different bead set using triazine chemistry.
30053875	10	100	theme	C	1660:1660	arg1	LPS					1662:1664	C LPS	1660:1664	C LPS	1660:1664	Results of the suspension array (BC1C1C2D) suggest that more pigs are seroconverted than indicated by a commercial BC1D1-ELISA, and that most of these extra seropositive samples give a signal on one of the beads with C LPS.
30053875	12	101	theme	variants	1960:1967	arg1	panel					1936:1940	the panel	1932:1940	the panel of Salmonella LPS variants, including C2 LPS	1932:1985	CONCLUSIONS A suspension array for Salmonella serology in pigs was developed, that detects more seropositive sera than ELISA, which is achieved by expanding the panel of Salmonella LPS variants, including C2 LPS.
30053875	14	102	theme	veterinary	2196:2205	arg1	serology					2207:2214	routine veterinary serology	2188:2214	routine veterinary serology	2188:2214	Ultimately, this type of assay can be applied in routine veterinary serology to test for antibodies against multiple Salmonella serovars (or other pathogens) in one single serum sample, using up-to-date antigen panels.
30053875	5	103	theme	broad	741:745	arg1	ELISA					747:751	a broad ELISA	739:751	a broad ELISA	739:751	Although C2 LPS may improve serology, use of C2 LPS in a broad ELISA was never achieved.
30053875	1	104	dep	pathogen	159:166	arg1	Salmonella					168:177	the zoonotic pathogen Salmonella	146:177	the zoonotic pathogen Salmonella	146:177	BACKGROUND Slaughter pigs are monitored for the presence of the zoonotic pathogen Salmonella, using both serology and bacteriology.
30053875	9	105	theme	analysis	1256:1263	arg1	combination					1237:1247	a combination	1235:1247	a combination of ROC analysis (B and D LPS) and a prevalence estimation based on historic data (C LPS)	1235:1336	With a combination of ROC analysis (B and D LPS) and a prevalence estimation based on historic data (C LPS), individual cut-offs were defined for each LPS-conjugated bead set, and assay performance was evaluated.
30053875	0	106	dep	array	24:28	arg1	detection					38:46	the detection	34:46	the detection of Salmonella antibodies in pig sera	34:83	A bead-based suspension array for the detection of Salmonella antibodies in pig sera.
30053875	12	107	theme	Salmonella	1945:1954	arg1	LPS					1983:1985	C2 LPS	1980:1985	C2 LPS	1980:1985	CONCLUSIONS A suspension array for Salmonella serology in pigs was developed, that detects more seropositive sera than ELISA, which is achieved by expanding the panel of Salmonella LPS variants, including C2 LPS.
30053875	12	107	theme	Salmonella	1945:1954	arg1	variants					1960:1967	Salmonella LPS variants	1945:1967	Salmonella LPS variants	1945:1967	CONCLUSIONS A suspension array for Salmonella serology in pigs was developed, that detects more seropositive sera than ELISA, which is achieved by expanding the panel of Salmonella LPS variants, including C2 LPS.
30053875	14	108	theme	assay	2164:2168	arg1	type					2156:2159	this type	2151:2159	this type of assay	2151:2168	Ultimately, this type of assay can be applied in routine veterinary serology to test for antibodies against multiple Salmonella serovars (or other pathogens) in one single serum sample, using up-to-date antigen panels.
30150092	12	0	theme	TRE	1579:1581	arg1	31792T					1602:1607	type strain TRE DT=DSM 103140T=JCM 31792T	1567:1607	type strain TRE DT=DSM 103140T=JCM 31792T	1567:1607	nov. (type strain TRE DT=DSM 103140T=JCM 31792T), Bifidobacterium felsineum sp.
30150092	12	0	theme	TRE	1579:1581	arg1	nov					1561:1563	nov	1561:1563	nov	1561:1563	nov. (type strain TRE DT=DSM 103140T=JCM 31792T), Bifidobacterium felsineum sp.
30150092	10	1	theme	TRE	1345:1347	arg1	HT					1349:1350	TRE HT	1345:1350	TRE HT	1345:1350	Based on the phylogenetic, genotypic and phenotypic data, the strains TRE 1T, TRE DT, TRE HT and TRI 7T clearly represent four novel taxa within the genus Bifidobacterium for which the names Bifidobacterium primatium sp.
30150092	10	1	theme	TRE	1345:1347	arg1	strains					1321:1327	the strains	1317:1327	the strains TRE 1T, TRE DT, TRE HT and TRI 7T	1317:1361	Based on the phylogenetic, genotypic and phenotypic data, the strains TRE 1T, TRE DT, TRE HT and TRI 7T clearly represent four novel taxa within the genus Bifidobacterium for which the names Bifidobacterium primatium sp.
30150092	4	2	dep	tamarin	206:212	arg1	oedipus					224:230	Saguinus oedipus	215:230	Saguinus oedipus	215:230	nov.: Four novel taxa isolated from the faeces of the cotton top tamarin (Saguinus oedipus) and the emperor tamarin (Saguinus imperator).
30150092	8	3	theme	independent	1058:1068	arg1	position					1083:1090	an independent phylogenetic position	1055:1090	an independent phylogenetic position of all strains with values ranged from 74 to 85% for ANI and from 24 to 28% for isDDH	1055:1176	The Average Nucleotide Identity (ANI) and in silico DDH (isDDH) analysis with closest neighbour supported an independent phylogenetic position of all strains with values ranged from 74 to 85% for ANI and from 24 to 28% for isDDH.
30150092	5	4	dep	novel	284:288	arg1	Gram-stain-positive					290:308	Gram-stain-positive	290:308	Gram-stain-positive	290:308	Four novel Gram-stain-positive, non spore forming and fructose-6-phosphate phosphoketolase-positive strains were isolated from the faeces of a cotton top tamarin (Saguinus oedipus) and an emperor tamarin (Saguinus imperator).
30150092	5	5	theme	emperor	467:473	arg1	tamarin					475:481	an emperor tamarin	464:481	an emperor tamarin (Saguinus imperator)	464:502	Four novel Gram-stain-positive, non spore forming and fructose-6-phosphate phosphoketolase-positive strains were isolated from the faeces of a cotton top tamarin (Saguinus oedipus) and an emperor tamarin (Saguinus imperator).
30150092	7	6	dep	values	833:838	arg1	7T					878:879	TRI 7T	874:879	similarity values of 97.4% and 97.5%, respectively; TRI 7T	822:879	longum ATCC 15708T with similarity values of 97.4% and 97.5%, respectively; TRI 7T was closely related to Bifidobacterium tissieri DSM 100201 (96.0%).
30150092	7	7	theme	ATCC	805:808	arg1	15708T					810:815	longum ATCC 15708T	798:815	longum ATCC 15708T with similarity values of 97.4% and 97.5%, respectively; TRI 7T	798:879	longum ATCC 15708T with similarity values of 97.4% and 97.5%, respectively; TRI 7T was closely related to Bifidobacterium tissieri DSM 100201 (96.0%).
30150092	6	8	theme	TRE	737:739	arg1	HT					741:742	TRE HT	737:742	TRE HT	737:742	Phylogenetic analyses based on 16S rRNA revealed that bifidobacterial strains TRE 1T exhibit close phylogenetic relatedness to Bifidobacterium catulorum DSM 103154 (96.0%) and Bifidobacterium tissieri DSM 100201 (96.0%); TRE DT and TRE HT were closely related to Bifidobacterium longum subsp.
30150092	10	9	theme	novel	1386:1390	arg1	taxa					1392:1395	four novel taxa	1381:1395	four novel taxa within the genus Bifidobacterium for which the names Bifidobacterium primatium sp	1381:1477	Based on the phylogenetic, genotypic and phenotypic data, the strains TRE 1T, TRE DT, TRE HT and TRI 7T clearly represent four novel taxa within the genus Bifidobacterium for which the names Bifidobacterium primatium sp.
30150092	4	10	theme	novel	152:156	arg1	taxa					158:161	Four novel taxa	147:161	nov.: Four novel taxa isolated from the faeces of the cotton top tamarin (Saguinus oedipus) and the emperor tamarin (Saguinus imperator).	141:277	nov.: Four novel taxa isolated from the faeces of the cotton top tamarin (Saguinus oedipus) and the emperor tamarin (Saguinus imperator).
30150092	8	11	with	Identity	972:979	arg1	neighbour					1035:1043	closest neighbour	1027:1043	closest neighbour	1027:1043	The Average Nucleotide Identity (ANI) and in silico DDH (isDDH) analysis with closest neighbour supported an independent phylogenetic position of all strains with values ranged from 74 to 85% for ANI and from 24 to 28% for isDDH.
30150092	8	12	with	strains	1099:1105	arg1	values					1112:1117	values	1112:1117	values ranged from 74 to 85% for ANI and from 24 to 28% for isDDH	1112:1176	The Average Nucleotide Identity (ANI) and in silico DDH (isDDH) analysis with closest neighbour supported an independent phylogenetic position of all strains with values ranged from 74 to 85% for ANI and from 24 to 28% for isDDH.
30150092	10	13	theme	genus	1408:1412	arg1	Bifidobacterium					1414:1428	the genus Bifidobacterium	1404:1428	the genus Bifidobacterium	1404:1428	Based on the phylogenetic, genotypic and phenotypic data, the strains TRE 1T, TRE DT, TRE HT and TRI 7T clearly represent four novel taxa within the genus Bifidobacterium for which the names Bifidobacterium primatium sp.
30150092	2	14	theme	felsineum	89:97	arg1	nov.					67:70	nov.	67:70	nov.	67:70	nov., Bifidobacterium felsineum sp.
30150092	2	14	theme	felsineum	89:97	arg1	sp					99:100	Bifidobacterium felsineum sp	73:100	Bifidobacterium felsineum sp	73:100	nov., Bifidobacterium felsineum sp.
30150092	14	15	theme	7T=DSM	1745:1750	arg1	31793					1764:1768	type strain TRI 7T=DSM 103153T=JCM 31793	1729:1768	type strain TRI 7T=DSM 103153T=JCM 31793	1729:1768	nov. (type strain TRI 7T=DSM 103153T=JCM 31793) are proposed.
30150092	14	15	theme	7T=DSM	1745:1750	arg1	nov					1723:1725	nov	1723:1725	nov	1723:1725	nov. (type strain TRI 7T=DSM 103153T=JCM 31793) are proposed.
30150092	11	16	theme	scaligerum	1546:1555	arg1	sp					1557:1558	Bifidobacterium scaligerum sp	1530:1558	Bifidobacterium scaligerum sp	1530:1558	nov. (type strain TRE 1T=DSM 100687T=JCM 30945T), Bifidobacterium scaligerum sp.
30150092	11	16	theme	scaligerum	1546:1555	arg1	nov					1480:1482	nov	1480:1482	nov	1480:1482	nov. (type strain TRE 1T=DSM 100687T=JCM 30945T), Bifidobacterium scaligerum sp.
30150092	6	17	theme	Bifidobacterium	632:646	arg1	%					674:674	96.0%	670:674	96.0%	670:674	Phylogenetic analyses based on 16S rRNA revealed that bifidobacterial strains TRE 1T exhibit close phylogenetic relatedness to Bifidobacterium catulorum DSM 103154 (96.0%) and Bifidobacterium tissieri DSM 100201 (96.0%); TRE DT and TRE HT were closely related to Bifidobacterium longum subsp.
30150092	6	17	theme	Bifidobacterium	632:646	arg1	DSM					658:660	Bifidobacterium catulorum DSM 103154	632:667	Bifidobacterium catulorum DSM 103154 (96.0%)	632:675	Phylogenetic analyses based on 16S rRNA revealed that bifidobacterial strains TRE 1T exhibit close phylogenetic relatedness to Bifidobacterium catulorum DSM 103154 (96.0%) and Bifidobacterium tissieri DSM 100201 (96.0%); TRE DT and TRE HT were closely related to Bifidobacterium longum subsp.
30150092	7	18	theme	Bifidobacterium	904:918	arg1	DSM					929:931	Bifidobacterium tissieri DSM 100201	904:938	Bifidobacterium tissieri DSM 100201 (96.0%)	904:946	longum ATCC 15708T with similarity values of 97.4% and 97.5%, respectively; TRI 7T was closely related to Bifidobacterium tissieri DSM 100201 (96.0%).
30150092	7	18	theme	Bifidobacterium	904:918	arg1	%					945:945	96.0%	941:945	96.0%	941:945	longum ATCC 15708T with similarity values of 97.4% and 97.5%, respectively; TRI 7T was closely related to Bifidobacterium tissieri DSM 100201 (96.0%).
30150092	4	19	theme	cotton	195:200	arg1	tamarin					206:212	the cotton top tamarin	191:212	the cotton top tamarin (Saguinus oedipus)	191:231	nov.: Four novel taxa isolated from the faeces of the cotton top tamarin (Saguinus oedipus) and the emperor tamarin (Saguinus imperator).
30150092	10	20	theme	genotypic	1286:1294	arg1	data					1311:1314	the phylogenetic, genotypic and phenotypic data	1268:1314	the phylogenetic, genotypic and phenotypic data	1268:1314	Based on the phylogenetic, genotypic and phenotypic data, the strains TRE 1T, TRE DT, TRE HT and TRI 7T clearly represent four novel taxa within the genus Bifidobacterium for which the names Bifidobacterium primatium sp.
30150092	13	21	theme	TRE	1659:1661	arg1	nov					1641:1643	nov	1641:1643	nov	1641:1643	nov. (type strain TRE HT=DSM 103139T=JCM 31789T) and Bifidobacterium simiarum sp.
30150092	13	21	theme	TRE	1659:1661	arg1	31789T					1682:1687	type strain TRE HT=DSM 103139T=JCM 31789T	1647:1687	type strain TRE HT=DSM 103139T=JCM 31789T	1647:1687	nov. (type strain TRE HT=DSM 103139T=JCM 31789T) and Bifidobacterium simiarum sp.
30150092	4	22	theme	tamarin	206:212	arg1	faeces					181:186	the faeces	177:186	the faeces of the cotton top tamarin (Saguinus oedipus) and the emperor tamarin (Saguinus imperator)	177:276	nov.: Four novel taxa isolated from the faeces of the cotton top tamarin (Saguinus oedipus) and the emperor tamarin (Saguinus imperator).
30150092	5	23	theme	fructose-6-phosphate	333:352	arg1	strains					379:385	fructose-6-phosphate phosphoketolase-positive strains	333:385	fructose-6-phosphate phosphoketolase-positive strains	333:385	Four novel Gram-stain-positive, non spore forming and fructose-6-phosphate phosphoketolase-positive strains were isolated from the faeces of a cotton top tamarin (Saguinus oedipus) and an emperor tamarin (Saguinus imperator).
30150092	10	24	theme	phylogenetic	1272:1283	arg1	data					1311:1314	the phylogenetic, genotypic and phenotypic data	1268:1314	the phylogenetic, genotypic and phenotypic data	1268:1314	Based on the phylogenetic, genotypic and phenotypic data, the strains TRE 1T, TRE DT, TRE HT and TRI 7T clearly represent four novel taxa within the genus Bifidobacterium for which the names Bifidobacterium primatium sp.
30150092	6	25	theme	close	598:602	arg1	relatedness					617:627	close phylogenetic relatedness	598:627	close phylogenetic relatedness to Bifidobacterium catulorum DSM 103154 (96.0%) and Bifidobacterium tissieri DSM 100201 (96.0%)	598:723	Phylogenetic analyses based on 16S rRNA revealed that bifidobacterial strains TRE 1T exhibit close phylogenetic relatedness to Bifidobacterium catulorum DSM 103154 (96.0%) and Bifidobacterium tissieri DSM 100201 (96.0%); TRE DT and TRE HT were closely related to Bifidobacterium longum subsp.
30150092	8	26	theme	Average	953:959	arg1	ANI					982:984	ANI	982:984	ANI	982:984	The Average Nucleotide Identity (ANI) and in silico DDH (isDDH) analysis with closest neighbour supported an independent phylogenetic position of all strains with values ranged from 74 to 85% for ANI and from 24 to 28% for isDDH.
30150092	8	26	theme	Average	953:959	arg1	Identity					972:979	The Average Nucleotide Identity	949:979	The Average Nucleotide Identity (ANI)	949:985	The Average Nucleotide Identity (ANI) and in silico DDH (isDDH) analysis with closest neighbour supported an independent phylogenetic position of all strains with values ranged from 74 to 85% for ANI and from 24 to 28% for isDDH.
30150092	5	27	dep	tamarin	433:439	arg1	oedipus					451:457	Saguinus oedipus	442:457	Saguinus oedipus	442:457	Four novel Gram-stain-positive, non spore forming and fructose-6-phosphate phosphoketolase-positive strains were isolated from the faeces of a cotton top tamarin (Saguinus oedipus) and an emperor tamarin (Saguinus imperator).
30150092	6	28	theme	tissieri	697:704	arg1	%					722:722	96.0%	718:722	96.0%	718:722	Phylogenetic analyses based on 16S rRNA revealed that bifidobacterial strains TRE 1T exhibit close phylogenetic relatedness to Bifidobacterium catulorum DSM 103154 (96.0%) and Bifidobacterium tissieri DSM 100201 (96.0%); TRE DT and TRE HT were closely related to Bifidobacterium longum subsp.
30150092	6	28	theme	tissieri	697:704	arg1	DSM					706:708	Bifidobacterium tissieri DSM 100201	681:715	Bifidobacterium tissieri DSM 100201 (96.0%)	681:723	Phylogenetic analyses based on 16S rRNA revealed that bifidobacterial strains TRE 1T exhibit close phylogenetic relatedness to Bifidobacterium catulorum DSM 103154 (96.0%) and Bifidobacterium tissieri DSM 100201 (96.0%); TRE DT and TRE HT were closely related to Bifidobacterium longum subsp.
30150092	9	29	theme	DNA	1179:1181	arg1	composition					1188:1198	DNA base composition	1179:1198	DNA base composition of the four strains	1179:1218	DNA base composition of the four strains was in the range of 58.3-63.5mol% G+C.
30150092	7	30	theme	TRI	874:876	arg1	7T					878:879	TRI 7T	874:879	similarity values of 97.4% and 97.5%, respectively; TRI 7T	822:879	longum ATCC 15708T with similarity values of 97.4% and 97.5%, respectively; TRI 7T was closely related to Bifidobacterium tissieri DSM 100201 (96.0%).
30150092	10	31	theme	phenotypic	1300:1309	arg1	data					1311:1314	the phylogenetic, genotypic and phenotypic data	1268:1314	the phylogenetic, genotypic and phenotypic data	1268:1314	Based on the phylogenetic, genotypic and phenotypic data, the strains TRE 1T, TRE DT, TRE HT and TRI 7T clearly represent four novel taxa within the genus Bifidobacterium for which the names Bifidobacterium primatium sp.
30150092	13	32	theme	type	1647:1650	arg1	nov					1641:1643	nov	1641:1643	nov	1641:1643	nov. (type strain TRE HT=DSM 103139T=JCM 31789T) and Bifidobacterium simiarum sp.
30150092	13	32	theme	type	1647:1650	arg1	31789T					1682:1687	type strain TRE HT=DSM 103139T=JCM 31789T	1647:1687	type strain TRE HT=DSM 103139T=JCM 31789T	1647:1687	nov. (type strain TRE HT=DSM 103139T=JCM 31789T) and Bifidobacterium simiarum sp.
30150092	0	33	theme	Bifidobacterium	0:14	arg1	sp					26:27	Bifidobacterium primatium sp	0:27	Bifidobacterium primatium sp.	0:28	Bifidobacterium primatium sp.
30150092	14	34	theme	103153T=JCM	1752:1762	arg1	31793					1764:1768	type strain TRI 7T=DSM 103153T=JCM 31793	1729:1768	type strain TRI 7T=DSM 103153T=JCM 31793	1729:1768	nov. (type strain TRI 7T=DSM 103153T=JCM 31793) are proposed.
30150092	14	34	theme	103153T=JCM	1752:1762	arg1	nov					1723:1725	nov	1723:1725	nov	1723:1725	nov. (type strain TRI 7T=DSM 103153T=JCM 31793) are proposed.
30150092	4	35	attach	isolated	163:170	arg1	faeces					181:186	the faeces	177:186	the faeces of the cotton top tamarin (Saguinus oedipus) and the emperor tamarin (Saguinus imperator)	177:276	nov.: Four novel taxa isolated from the faeces of the cotton top tamarin (Saguinus oedipus) and the emperor tamarin (Saguinus imperator).
30150092	4	35	attach	isolated	163:170	arg2	taxa					158:161	Four novel taxa	147:161	nov.: Four novel taxa isolated from the faeces of the cotton top tamarin (Saguinus oedipus) and the emperor tamarin (Saguinus imperator).	141:277	nov.: Four novel taxa isolated from the faeces of the cotton top tamarin (Saguinus oedipus) and the emperor tamarin (Saguinus imperator).
30150092	14	36	theme	strain	1734:1739	arg1	31793					1764:1768	type strain TRI 7T=DSM 103153T=JCM 31793	1729:1768	type strain TRI 7T=DSM 103153T=JCM 31793	1729:1768	nov. (type strain TRI 7T=DSM 103153T=JCM 31793) are proposed.
30150092	14	36	theme	strain	1734:1739	arg1	nov					1723:1725	nov	1723:1725	nov	1723:1725	nov. (type strain TRI 7T=DSM 103153T=JCM 31793) are proposed.
30150092	14	37	theme	TRI	1741:1743	arg1	31793					1764:1768	type strain TRI 7T=DSM 103153T=JCM 31793	1729:1768	type strain TRI 7T=DSM 103153T=JCM 31793	1729:1768	nov. (type strain TRI 7T=DSM 103153T=JCM 31793) are proposed.
30150092	14	37	theme	TRI	1741:1743	arg1	nov					1723:1725	nov	1723:1725	nov	1723:1725	nov. (type strain TRI 7T=DSM 103153T=JCM 31793) are proposed.
30150092	4	38	theme	tamarin	249:255	arg1	faeces					181:186	the faeces	177:186	the faeces of the cotton top tamarin (Saguinus oedipus) and the emperor tamarin (Saguinus imperator)	177:276	nov.: Four novel taxa isolated from the faeces of the cotton top tamarin (Saguinus oedipus) and the emperor tamarin (Saguinus imperator).
30150092	7	39	theme	similarity	822:831	arg1	values					833:838	similarity values	822:838	similarity values of 97.4% and 97.5%, respectively; TRI 7T	822:879	longum ATCC 15708T with similarity values of 97.4% and 97.5%, respectively; TRI 7T was closely related to Bifidobacterium tissieri DSM 100201 (96.0%).
30150092	11	40	theme	TRE	1498:1500	arg1	nov					1480:1482	nov	1480:1482	nov	1480:1482	nov. (type strain TRE 1T=DSM 100687T=JCM 30945T), Bifidobacterium scaligerum sp.
30150092	11	40	theme	TRE	1498:1500	arg1	30945T					1521:1526	type strain TRE 1T=DSM 100687T=JCM 30945T	1486:1526	type strain TRE 1T=DSM 100687T=JCM 30945T	1486:1526	nov. (type strain TRE 1T=DSM 100687T=JCM 30945T), Bifidobacterium scaligerum sp.
30150092	9	41	theme	strains	1212:1218	arg1	composition					1188:1198	DNA base composition	1179:1198	DNA base composition of the four strains	1179:1218	DNA base composition of the four strains was in the range of 58.3-63.5mol% G+C.
30150092	14	42	theme	type	1729:1732	arg1	31793					1764:1768	type strain TRI 7T=DSM 103153T=JCM 31793	1729:1768	type strain TRI 7T=DSM 103153T=JCM 31793	1729:1768	nov. (type strain TRI 7T=DSM 103153T=JCM 31793) are proposed.
30150092	14	42	theme	type	1729:1732	arg1	nov					1723:1725	nov	1723:1725	nov	1723:1725	nov. (type strain TRI 7T=DSM 103153T=JCM 31793) are proposed.
30150092	11	43	theme	type	1486:1489	arg1	nov					1480:1482	nov	1480:1482	nov	1480:1482	nov. (type strain TRE 1T=DSM 100687T=JCM 30945T), Bifidobacterium scaligerum sp.
30150092	11	43	theme	type	1486:1489	arg1	30945T					1521:1526	type strain TRE 1T=DSM 100687T=JCM 30945T	1486:1526	type strain TRE 1T=DSM 100687T=JCM 30945T	1486:1526	nov. (type strain TRE 1T=DSM 100687T=JCM 30945T), Bifidobacterium scaligerum sp.
30150092	6	44	theme	TRE	726:728	arg1	DT					730:731	TRE DT	726:731	TRE DT	726:731	Phylogenetic analyses based on 16S rRNA revealed that bifidobacterial strains TRE 1T exhibit close phylogenetic relatedness to Bifidobacterium catulorum DSM 103154 (96.0%) and Bifidobacterium tissieri DSM 100201 (96.0%); TRE DT and TRE HT were closely related to Bifidobacterium longum subsp.
30150092	12	45	theme	Bifidobacterium	1611:1625	arg1	sp					1637:1638	Bifidobacterium felsineum sp	1611:1638	Bifidobacterium felsineum sp	1611:1638	nov. (type strain TRE DT=DSM 103140T=JCM 31792T), Bifidobacterium felsineum sp.
30150092	12	45	theme	Bifidobacterium	1611:1625	arg1	nov					1561:1563	nov	1561:1563	nov	1561:1563	nov. (type strain TRE DT=DSM 103140T=JCM 31792T), Bifidobacterium felsineum sp.
30150092	10	46	theme	TRE	1337:1339	arg1	DT					1341:1342	TRE DT	1337:1342	TRE DT	1337:1342	Based on the phylogenetic, genotypic and phenotypic data, the strains TRE 1T, TRE DT, TRE HT and TRI 7T clearly represent four novel taxa within the genus Bifidobacterium for which the names Bifidobacterium primatium sp.
30150092	10	46	theme	TRE	1337:1339	arg1	strains					1321:1327	the strains	1317:1327	the strains TRE 1T, TRE DT, TRE HT and TRI 7T	1317:1361	Based on the phylogenetic, genotypic and phenotypic data, the strains TRE 1T, TRE DT, TRE HT and TRI 7T clearly represent four novel taxa within the genus Bifidobacterium for which the names Bifidobacterium primatium sp.
30150092	5	47	theme	tamarin	433:439	arg1	faeces					410:415	the faeces	406:415	the faeces of a cotton top tamarin (Saguinus oedipus) and an emperor tamarin (Saguinus imperator)	406:502	Four novel Gram-stain-positive, non spore forming and fructose-6-phosphate phosphoketolase-positive strains were isolated from the faeces of a cotton top tamarin (Saguinus oedipus) and an emperor tamarin (Saguinus imperator).
30150092	12	48	theme	strain	1572:1577	arg1	31792T					1602:1607	type strain TRE DT=DSM 103140T=JCM 31792T	1567:1607	type strain TRE DT=DSM 103140T=JCM 31792T	1567:1607	nov. (type strain TRE DT=DSM 103140T=JCM 31792T), Bifidobacterium felsineum sp.
30150092	12	48	theme	strain	1572:1577	arg1	nov					1561:1563	nov	1561:1563	nov	1561:1563	nov. (type strain TRE DT=DSM 103140T=JCM 31792T), Bifidobacterium felsineum sp.
30150092	10	49	theme	TRI	1356:1358	arg1	strains					1321:1327	the strains	1317:1327	the strains TRE 1T, TRE DT, TRE HT and TRI 7T	1317:1361	Based on the phylogenetic, genotypic and phenotypic data, the strains TRE 1T, TRE DT, TRE HT and TRI 7T clearly represent four novel taxa within the genus Bifidobacterium for which the names Bifidobacterium primatium sp.
30150092	10	49	theme	TRI	1356:1358	arg1	7T					1360:1361	TRI 7T	1356:1361	TRI 7T	1356:1361	Based on the phylogenetic, genotypic and phenotypic data, the strains TRE 1T, TRE DT, TRE HT and TRI 7T clearly represent four novel taxa within the genus Bifidobacterium for which the names Bifidobacterium primatium sp.
30150092	11	50	theme	100687T=JCM	1509:1519	arg1	nov					1480:1482	nov	1480:1482	nov	1480:1482	nov. (type strain TRE 1T=DSM 100687T=JCM 30945T), Bifidobacterium scaligerum sp.
30150092	11	50	theme	100687T=JCM	1509:1519	arg1	30945T					1521:1526	type strain TRE 1T=DSM 100687T=JCM 30945T	1486:1526	type strain TRE 1T=DSM 100687T=JCM 30945T	1486:1526	nov. (type strain TRE 1T=DSM 100687T=JCM 30945T), Bifidobacterium scaligerum sp.
30150092	4	51	dep	imperator	267:275	arg1	Saguinus					258:265	Saguinus	258:265	Saguinus	258:265	nov.: Four novel taxa isolated from the faeces of the cotton top tamarin (Saguinus oedipus) and the emperor tamarin (Saguinus imperator).
30150092	9	52	theme	58.3-63.5mol	1240:1251	arg1	G+C					1254:1256	58.3-63.5mol% G+C	1240:1256	58.3-63.5mol% G+C	1240:1256	DNA base composition of the four strains was in the range of 58.3-63.5mol% G+C.
30150092	1	53	theme	scaligerum	52:61	arg1	sp					63:64	Bifidobacterium scaligerum sp	36:64	Bifidobacterium scaligerum sp	36:64	nov., Bifidobacterium scaligerum sp.
30150092	1	53	theme	scaligerum	52:61	arg1	nov.					30:33	nov.	30:33	nov.	30:33	nov., Bifidobacterium scaligerum sp.
30150092	12	54	theme	DT=DSM	1583:1588	arg1	31792T					1602:1607	type strain TRE DT=DSM 103140T=JCM 31792T	1567:1607	type strain TRE DT=DSM 103140T=JCM 31792T	1567:1607	nov. (type strain TRE DT=DSM 103140T=JCM 31792T), Bifidobacterium felsineum sp.
30150092	12	54	theme	DT=DSM	1583:1588	arg1	nov					1561:1563	nov	1561:1563	nov	1561:1563	nov. (type strain TRE DT=DSM 103140T=JCM 31792T), Bifidobacterium felsineum sp.
30150092	8	55	with	analysis	1013:1020	arg1	neighbour					1035:1043	closest neighbour	1027:1043	closest neighbour	1027:1043	The Average Nucleotide Identity (ANI) and in silico DDH (isDDH) analysis with closest neighbour supported an independent phylogenetic position of all strains with values ranged from 74 to 85% for ANI and from 24 to 28% for isDDH.
30150092	7	56	theme	%	847:847	arg1	values					833:838	similarity values	822:838	similarity values of 97.4% and 97.5%, respectively; TRI 7T	822:879	longum ATCC 15708T with similarity values of 97.4% and 97.5%, respectively; TRI 7T was closely related to Bifidobacterium tissieri DSM 100201 (96.0%).
30150092	9	57	theme	G+C	1254:1256	arg1	range					1231:1235	the range	1227:1235	the range of 58.3-63.5mol% G+C	1227:1256	DNA base composition of the four strains was in the range of 58.3-63.5mol% G+C.
30150092	6	58	theme	Phylogenetic	505:516	arg1	analyses					518:525	Phylogenetic analyses	505:525	Phylogenetic analyses based on 16S rRNA	505:543	Phylogenetic analyses based on 16S rRNA revealed that bifidobacterial strains TRE 1T exhibit close phylogenetic relatedness to Bifidobacterium catulorum DSM 103154 (96.0%) and Bifidobacterium tissieri DSM 100201 (96.0%); TRE DT and TRE HT were closely related to Bifidobacterium longum subsp.
30150092	5	59	theme	cotton	422:427	arg1	tamarin					433:439	a cotton top tamarin	420:439	a cotton top tamarin (Saguinus oedipus)	420:458	Four novel Gram-stain-positive, non spore forming and fructose-6-phosphate phosphoketolase-positive strains were isolated from the faeces of a cotton top tamarin (Saguinus oedipus) and an emperor tamarin (Saguinus imperator).
30150092	5	60	theme	tamarin	475:481	arg1	faeces					410:415	the faeces	406:415	the faeces of a cotton top tamarin (Saguinus oedipus) and an emperor tamarin (Saguinus imperator)	406:502	Four novel Gram-stain-positive, non spore forming and fructose-6-phosphate phosphoketolase-positive strains were isolated from the faeces of a cotton top tamarin (Saguinus oedipus) and an emperor tamarin (Saguinus imperator).
30150092	8	61	theme	phylogenetic	1070:1081	arg1	position					1083:1090	an independent phylogenetic position	1055:1090	an independent phylogenetic position of all strains with values ranged from 74 to 85% for ANI and from 24 to 28% for isDDH	1055:1176	The Average Nucleotide Identity (ANI) and in silico DDH (isDDH) analysis with closest neighbour supported an independent phylogenetic position of all strains with values ranged from 74 to 85% for ANI and from 24 to 28% for isDDH.
30150092	6	62	theme	TRE	583:585	arg1	1T					587:588	TRE 1T	583:588	bifidobacterial strains TRE 1T	559:588	Phylogenetic analyses based on 16S rRNA revealed that bifidobacterial strains TRE 1T exhibit close phylogenetic relatedness to Bifidobacterium catulorum DSM 103154 (96.0%) and Bifidobacterium tissieri DSM 100201 (96.0%); TRE DT and TRE HT were closely related to Bifidobacterium longum subsp.
30150092	7	63	theme	longum	798:803	arg1	15708T					810:815	longum ATCC 15708T	798:815	longum ATCC 15708T with similarity values of 97.4% and 97.5%, respectively; TRI 7T	798:879	longum ATCC 15708T with similarity values of 97.4% and 97.5%, respectively; TRI 7T was closely related to Bifidobacterium tissieri DSM 100201 (96.0%).
30150092	8	64	theme	strains	1099:1105	arg1	position					1083:1090	an independent phylogenetic position	1055:1090	an independent phylogenetic position of all strains with values ranged from 74 to 85% for ANI and from 24 to 28% for isDDH	1055:1176	The Average Nucleotide Identity (ANI) and in silico DDH (isDDH) analysis with closest neighbour supported an independent phylogenetic position of all strains with values ranged from 74 to 85% for ANI and from 24 to 28% for isDDH.
30150092	5	65	dep	imperator	493:501	arg1	Saguinus					484:491	Saguinus	484:491	Saguinus	484:491	Four novel Gram-stain-positive, non spore forming and fructose-6-phosphate phosphoketolase-positive strains were isolated from the faeces of a cotton top tamarin (Saguinus oedipus) and an emperor tamarin (Saguinus imperator).
30150092	6	66	theme	bifidobacterial	559:573	arg1	strains					575:581	bifidobacterial strains	559:581	bifidobacterial strains TRE 1T	559:588	Phylogenetic analyses based on 16S rRNA revealed that bifidobacterial strains TRE 1T exhibit close phylogenetic relatedness to Bifidobacterium catulorum DSM 103154 (96.0%) and Bifidobacterium tissieri DSM 100201 (96.0%); TRE DT and TRE HT were closely related to Bifidobacterium longum subsp.
30150092	13	67	theme	HT=DSM	1663:1668	arg1	nov					1641:1643	nov	1641:1643	nov	1641:1643	nov. (type strain TRE HT=DSM 103139T=JCM 31789T) and Bifidobacterium simiarum sp.
30150092	13	67	theme	HT=DSM	1663:1668	arg1	31789T					1682:1687	type strain TRE HT=DSM 103139T=JCM 31789T	1647:1687	type strain TRE HT=DSM 103139T=JCM 31789T	1647:1687	nov. (type strain TRE HT=DSM 103139T=JCM 31789T) and Bifidobacterium simiarum sp.
30150092	5	68	theme	top	429:431	arg1	tamarin					433:439	a cotton top tamarin	420:439	a cotton top tamarin (Saguinus oedipus)	420:458	Four novel Gram-stain-positive, non spore forming and fructose-6-phosphate phosphoketolase-positive strains were isolated from the faeces of a cotton top tamarin (Saguinus oedipus) and an emperor tamarin (Saguinus imperator).
30150092	3	69	theme	Bifidobacterium	112:126	arg1	simiarum					128:135	Bifidobacterium simiarum	112:135	Bifidobacterium simiarum	112:135	nov. and Bifidobacterium simiarum sp.
30150092	8	70	dep	in	991:992	arg1	silico					994:999	silico	994:999	silico	994:999	The Average Nucleotide Identity (ANI) and in silico DDH (isDDH) analysis with closest neighbour supported an independent phylogenetic position of all strains with values ranged from 74 to 85% for ANI and from 24 to 28% for isDDH.
30150092	12	71	theme	felsineum	1627:1635	arg1	sp					1637:1638	Bifidobacterium felsineum sp	1611:1638	Bifidobacterium felsineum sp	1611:1638	nov. (type strain TRE DT=DSM 103140T=JCM 31792T), Bifidobacterium felsineum sp.
30150092	12	71	theme	felsineum	1627:1635	arg1	nov					1561:1563	nov	1561:1563	nov	1561:1563	nov. (type strain TRE DT=DSM 103140T=JCM 31792T), Bifidobacterium felsineum sp.
30150092	6	72	dep	strains	575:581	arg1	1T					587:588	TRE 1T	583:588	bifidobacterial strains TRE 1T	559:588	Phylogenetic analyses based on 16S rRNA revealed that bifidobacterial strains TRE 1T exhibit close phylogenetic relatedness to Bifidobacterium catulorum DSM 103154 (96.0%) and Bifidobacterium tissieri DSM 100201 (96.0%); TRE DT and TRE HT were closely related to Bifidobacterium longum subsp.
30150092	2	73	theme	Bifidobacterium	73:87	arg1	nov.					67:70	nov.	67:70	nov.	67:70	nov., Bifidobacterium felsineum sp.
30150092	2	73	theme	Bifidobacterium	73:87	arg1	sp					99:100	Bifidobacterium felsineum sp	73:100	Bifidobacterium felsineum sp	73:100	nov., Bifidobacterium felsineum sp.
30150092	13	74	dep	sp	1719:1720	arg1	simiarum					1710:1717	Bifidobacterium simiarum	1694:1717	Bifidobacterium simiarum	1694:1717	nov. (type strain TRE HT=DSM 103139T=JCM 31789T) and Bifidobacterium simiarum sp.
30150092	13	74	dep	sp	1719:1720	arg1	31789T					1682:1687	type strain TRE HT=DSM 103139T=JCM 31789T	1647:1687	type strain TRE HT=DSM 103139T=JCM 31789T	1647:1687	nov. (type strain TRE HT=DSM 103139T=JCM 31789T) and Bifidobacterium simiarum sp.
30150092	13	74	dep	sp	1719:1720	arg1	nov					1641:1643	nov	1641:1643	nov	1641:1643	nov. (type strain TRE HT=DSM 103139T=JCM 31789T) and Bifidobacterium simiarum sp.
30150092	6	75	theme	catulorum	648:656	arg1	%					674:674	96.0%	670:674	96.0%	670:674	Phylogenetic analyses based on 16S rRNA revealed that bifidobacterial strains TRE 1T exhibit close phylogenetic relatedness to Bifidobacterium catulorum DSM 103154 (96.0%) and Bifidobacterium tissieri DSM 100201 (96.0%); TRE DT and TRE HT were closely related to Bifidobacterium longum subsp.
30150092	6	75	theme	catulorum	648:656	arg1	DSM					658:660	Bifidobacterium catulorum DSM 103154	632:667	Bifidobacterium catulorum DSM 103154 (96.0%)	632:675	Phylogenetic analyses based on 16S rRNA revealed that bifidobacterial strains TRE 1T exhibit close phylogenetic relatedness to Bifidobacterium catulorum DSM 103154 (96.0%) and Bifidobacterium tissieri DSM 100201 (96.0%); TRE DT and TRE HT were closely related to Bifidobacterium longum subsp.
30150092	13	76	theme	103139T=JCM	1670:1680	arg1	nov					1641:1643	nov	1641:1643	nov	1641:1643	nov. (type strain TRE HT=DSM 103139T=JCM 31789T) and Bifidobacterium simiarum sp.
30150092	13	76	theme	103139T=JCM	1670:1680	arg1	31789T					1682:1687	type strain TRE HT=DSM 103139T=JCM 31789T	1647:1687	type strain TRE HT=DSM 103139T=JCM 31789T	1647:1687	nov. (type strain TRE HT=DSM 103139T=JCM 31789T) and Bifidobacterium simiarum sp.
30150092	13	77	theme	strain	1652:1657	arg1	nov					1641:1643	nov	1641:1643	nov	1641:1643	nov. (type strain TRE HT=DSM 103139T=JCM 31789T) and Bifidobacterium simiarum sp.
30150092	13	77	theme	strain	1652:1657	arg1	31789T					1682:1687	type strain TRE HT=DSM 103139T=JCM 31789T	1647:1687	type strain TRE HT=DSM 103139T=JCM 31789T	1647:1687	nov. (type strain TRE HT=DSM 103139T=JCM 31789T) and Bifidobacterium simiarum sp.
30150092	11	78	theme	Bifidobacterium	1530:1544	arg1	sp					1557:1558	Bifidobacterium scaligerum sp	1530:1558	Bifidobacterium scaligerum sp	1530:1558	nov. (type strain TRE 1T=DSM 100687T=JCM 30945T), Bifidobacterium scaligerum sp.
30150092	11	78	theme	Bifidobacterium	1530:1544	arg1	nov					1480:1482	nov	1480:1482	nov	1480:1482	nov. (type strain TRE 1T=DSM 100687T=JCM 30945T), Bifidobacterium scaligerum sp.
30150092	7	79	theme	tissieri	920:927	arg1	DSM					929:931	Bifidobacterium tissieri DSM 100201	904:938	Bifidobacterium tissieri DSM 100201 (96.0%)	904:946	longum ATCC 15708T with similarity values of 97.4% and 97.5%, respectively; TRI 7T was closely related to Bifidobacterium tissieri DSM 100201 (96.0%).
30150092	7	79	theme	tissieri	920:927	arg1	%					945:945	96.0%	941:945	96.0%	941:945	longum ATCC 15708T with similarity values of 97.4% and 97.5%, respectively; TRI 7T was closely related to Bifidobacterium tissieri DSM 100201 (96.0%).
30150092	6	80	theme	phylogenetic	604:615	arg1	relatedness					617:627	close phylogenetic relatedness	598:627	close phylogenetic relatedness to Bifidobacterium catulorum DSM 103154 (96.0%) and Bifidobacterium tissieri DSM 100201 (96.0%)	598:723	Phylogenetic analyses based on 16S rRNA revealed that bifidobacterial strains TRE 1T exhibit close phylogenetic relatedness to Bifidobacterium catulorum DSM 103154 (96.0%) and Bifidobacterium tissieri DSM 100201 (96.0%); TRE DT and TRE HT were closely related to Bifidobacterium longum subsp.
30150092	10	81	dep	strains	1321:1327	arg1	1T					1333:1334	TRE 1T	1329:1334	TRE 1T	1329:1334	Based on the phylogenetic, genotypic and phenotypic data, the strains TRE 1T, TRE DT, TRE HT and TRI 7T clearly represent four novel taxa within the genus Bifidobacterium for which the names Bifidobacterium primatium sp.
30150092	10	81	dep	strains	1321:1327	arg1	DT					1341:1342	TRE DT	1337:1342	TRE DT	1337:1342	Based on the phylogenetic, genotypic and phenotypic data, the strains TRE 1T, TRE DT, TRE HT and TRI 7T clearly represent four novel taxa within the genus Bifidobacterium for which the names Bifidobacterium primatium sp.
30150092	10	81	dep	strains	1321:1327	arg1	HT					1349:1350	TRE HT	1345:1350	TRE HT	1345:1350	Based on the phylogenetic, genotypic and phenotypic data, the strains TRE 1T, TRE DT, TRE HT and TRI 7T clearly represent four novel taxa within the genus Bifidobacterium for which the names Bifidobacterium primatium sp.
30150092	10	81	dep	strains	1321:1327	arg1	7T					1360:1361	TRI 7T	1356:1361	TRI 7T	1356:1361	Based on the phylogenetic, genotypic and phenotypic data, the strains TRE 1T, TRE DT, TRE HT and TRI 7T clearly represent four novel taxa within the genus Bifidobacterium for which the names Bifidobacterium primatium sp.
30150092	10	81	dep	strains	1321:1327	arg1	strains					1321:1327	the strains	1317:1327	the strains TRE 1T, TRE DT, TRE HT and TRI 7T	1317:1361	Based on the phylogenetic, genotypic and phenotypic data, the strains TRE 1T, TRE DT, TRE HT and TRI 7T clearly represent four novel taxa within the genus Bifidobacterium for which the names Bifidobacterium primatium sp.
30150092	8	82	theme	Nucleotide	961:970	arg1	ANI					982:984	ANI	982:984	ANI	982:984	The Average Nucleotide Identity (ANI) and in silico DDH (isDDH) analysis with closest neighbour supported an independent phylogenetic position of all strains with values ranged from 74 to 85% for ANI and from 24 to 28% for isDDH.
30150092	8	82	theme	Nucleotide	961:970	arg1	Identity					972:979	The Average Nucleotide Identity	949:979	The Average Nucleotide Identity (ANI)	949:985	The Average Nucleotide Identity (ANI) and in silico DDH (isDDH) analysis with closest neighbour supported an independent phylogenetic position of all strains with values ranged from 74 to 85% for ANI and from 24 to 28% for isDDH.
30150092	4	83	theme	top	202:204	arg1	tamarin					206:212	the cotton top tamarin	191:212	the cotton top tamarin (Saguinus oedipus)	191:231	nov.: Four novel taxa isolated from the faeces of the cotton top tamarin (Saguinus oedipus) and the emperor tamarin (Saguinus imperator).
30150092	5	84	theme	phosphoketolase-positive	354:377	arg1	strains					379:385	fructose-6-phosphate phosphoketolase-positive strains	333:385	fructose-6-phosphate phosphoketolase-positive strains	333:385	Four novel Gram-stain-positive, non spore forming and fructose-6-phosphate phosphoketolase-positive strains were isolated from the faeces of a cotton top tamarin (Saguinus oedipus) and an emperor tamarin (Saguinus imperator).
30150092	6	85	theme	Bifidobacterium	681:695	arg1	%					722:722	96.0%	718:722	96.0%	718:722	Phylogenetic analyses based on 16S rRNA revealed that bifidobacterial strains TRE 1T exhibit close phylogenetic relatedness to Bifidobacterium catulorum DSM 103154 (96.0%) and Bifidobacterium tissieri DSM 100201 (96.0%); TRE DT and TRE HT were closely related to Bifidobacterium longum subsp.
30150092	6	85	theme	Bifidobacterium	681:695	arg1	DSM					706:708	Bifidobacterium tissieri DSM 100201	681:715	Bifidobacterium tissieri DSM 100201 (96.0%)	681:723	Phylogenetic analyses based on 16S rRNA revealed that bifidobacterial strains TRE 1T exhibit close phylogenetic relatedness to Bifidobacterium catulorum DSM 103154 (96.0%) and Bifidobacterium tissieri DSM 100201 (96.0%); TRE DT and TRE HT were closely related to Bifidobacterium longum subsp.
30150092	10	86	theme	Bifidobacterium	1450:1464	arg1	sp					1476:1477	Bifidobacterium primatium sp	1450:1477	Bifidobacterium primatium sp	1450:1477	Based on the phylogenetic, genotypic and phenotypic data, the strains TRE 1T, TRE DT, TRE HT and TRI 7T clearly represent four novel taxa within the genus Bifidobacterium for which the names Bifidobacterium primatium sp.
30150092	0	87	theme	primatium	16:24	arg1	sp					26:27	Bifidobacterium primatium sp	0:27	Bifidobacterium primatium sp.	0:28	Bifidobacterium primatium sp.
30150092	9	88	theme	base	1183:1186	arg1	composition					1188:1198	DNA base composition	1179:1198	DNA base composition of the four strains	1179:1218	DNA base composition of the four strains was in the range of 58.3-63.5mol% G+C.
30150092	6	89	theme	16S	536:538	arg1	rRNA					540:543	16S rRNA	536:543	16S rRNA	536:543	Phylogenetic analyses based on 16S rRNA revealed that bifidobacterial strains TRE 1T exhibit close phylogenetic relatedness to Bifidobacterium catulorum DSM 103154 (96.0%) and Bifidobacterium tissieri DSM 100201 (96.0%); TRE DT and TRE HT were closely related to Bifidobacterium longum subsp.
30150092	3	90	dep	sp	137:138	arg1	nov.					103:106	nov.	103:106	nov.	103:106	nov. and Bifidobacterium simiarum sp.
30150092	3	90	dep	sp	137:138	arg1	simiarum					128:135	Bifidobacterium simiarum	112:135	Bifidobacterium simiarum	112:135	nov. and Bifidobacterium simiarum sp.
30150092	1	91	theme	Bifidobacterium	36:50	arg1	sp					63:64	Bifidobacterium scaligerum sp	36:64	Bifidobacterium scaligerum sp	36:64	nov., Bifidobacterium scaligerum sp.
30150092	1	91	theme	Bifidobacterium	36:50	arg1	nov.					30:33	nov.	30:33	nov.	30:33	nov., Bifidobacterium scaligerum sp.
30150092	8	92	theme	in	991:992	arg1	analysis					1013:1020	in silico DDH (isDDH) analysis	991:1020	in silico DDH (isDDH) analysis	991:1020	The Average Nucleotide Identity (ANI) and in silico DDH (isDDH) analysis with closest neighbour supported an independent phylogenetic position of all strains with values ranged from 74 to 85% for ANI and from 24 to 28% for isDDH.
30150092	8	93	dep	85	1137:1138	arg1	to					1134:1135	to	1134:1135	to	1134:1135	The Average Nucleotide Identity (ANI) and in silico DDH (isDDH) analysis with closest neighbour supported an independent phylogenetic position of all strains with values ranged from 74 to 85% for ANI and from 24 to 28% for isDDH.
30150092	10	94	theme	TRE	1329:1331	arg1	1T					1333:1334	TRE 1T	1329:1334	TRE 1T	1329:1334	Based on the phylogenetic, genotypic and phenotypic data, the strains TRE 1T, TRE DT, TRE HT and TRI 7T clearly represent four novel taxa within the genus Bifidobacterium for which the names Bifidobacterium primatium sp.
30150092	10	94	theme	TRE	1329:1331	arg1	strains					1321:1327	the strains	1317:1327	the strains TRE 1T, TRE DT, TRE HT and TRI 7T	1317:1361	Based on the phylogenetic, genotypic and phenotypic data, the strains TRE 1T, TRE DT, TRE HT and TRI 7T clearly represent four novel taxa within the genus Bifidobacterium for which the names Bifidobacterium primatium sp.
30150092	11	95	theme	strain	1491:1496	arg1	nov					1480:1482	nov	1480:1482	nov	1480:1482	nov. (type strain TRE 1T=DSM 100687T=JCM 30945T), Bifidobacterium scaligerum sp.
30150092	11	95	theme	strain	1491:1496	arg1	30945T					1521:1526	type strain TRE 1T=DSM 100687T=JCM 30945T	1486:1526	type strain TRE 1T=DSM 100687T=JCM 30945T	1486:1526	nov. (type strain TRE 1T=DSM 100687T=JCM 30945T), Bifidobacterium scaligerum sp.
30150092	8	96	theme	DDH	1001:1003	arg1	analysis					1013:1020	in silico DDH (isDDH) analysis	991:1020	in silico DDH (isDDH) analysis	991:1020	The Average Nucleotide Identity (ANI) and in silico DDH (isDDH) analysis with closest neighbour supported an independent phylogenetic position of all strains with values ranged from 74 to 85% for ANI and from 24 to 28% for isDDH.
30150092	5	97	attach	isolated	392:399	arg1	faeces					410:415	the faeces	406:415	the faeces of a cotton top tamarin (Saguinus oedipus) and an emperor tamarin (Saguinus imperator)	406:502	Four novel Gram-stain-positive, non spore forming and fructose-6-phosphate phosphoketolase-positive strains were isolated from the faeces of a cotton top tamarin (Saguinus oedipus) and an emperor tamarin (Saguinus imperator).
30150092	5	97	attach	isolated	392:399	arg2	spore					315:319	spore	315:319	spore	315:319	Four novel Gram-stain-positive, non spore forming and fructose-6-phosphate phosphoketolase-positive strains were isolated from the faeces of a cotton top tamarin (Saguinus oedipus) and an emperor tamarin (Saguinus imperator).
30150092	5	97	attach	isolated	392:399	arg2	strains					379:385	fructose-6-phosphate phosphoketolase-positive strains	333:385	fructose-6-phosphate phosphoketolase-positive strains	333:385	Four novel Gram-stain-positive, non spore forming and fructose-6-phosphate phosphoketolase-positive strains were isolated from the faeces of a cotton top tamarin (Saguinus oedipus) and an emperor tamarin (Saguinus imperator).
30150092	5	97	attach	isolated	392:399	arg2	Four					279:282	Four	279:282	Four	279:282	Four novel Gram-stain-positive, non spore forming and fructose-6-phosphate phosphoketolase-positive strains were isolated from the faeces of a cotton top tamarin (Saguinus oedipus) and an emperor tamarin (Saguinus imperator).
30150092	4	98	theme	emperor	241:247	arg1	tamarin					249:255	the emperor tamarin	237:255	the emperor tamarin (Saguinus imperator)	237:276	nov.: Four novel taxa isolated from the faeces of the cotton top tamarin (Saguinus oedipus) and the emperor tamarin (Saguinus imperator).
30150092	10	99	dep	sp	1476:1477	arg1	names					1444:1448	the names	1440:1448	the names	1440:1448	Based on the phylogenetic, genotypic and phenotypic data, the strains TRE 1T, TRE DT, TRE HT and TRI 7T clearly represent four novel taxa within the genus Bifidobacterium for which the names Bifidobacterium primatium sp.
30150092	8	100	theme	isDDH	1006:1010	arg1	analysis					1013:1020	in silico DDH (isDDH) analysis	991:1020	in silico DDH (isDDH) analysis	991:1020	The Average Nucleotide Identity (ANI) and in silico DDH (isDDH) analysis with closest neighbour supported an independent phylogenetic position of all strains with values ranged from 74 to 85% for ANI and from 24 to 28% for isDDH.
30150092	12	101	theme	103140T=JCM	1590:1600	arg1	31792T					1602:1607	type strain TRE DT=DSM 103140T=JCM 31792T	1567:1607	type strain TRE DT=DSM 103140T=JCM 31792T	1567:1607	nov. (type strain TRE DT=DSM 103140T=JCM 31792T), Bifidobacterium felsineum sp.
30150092	12	101	theme	103140T=JCM	1590:1600	arg1	nov					1561:1563	nov	1561:1563	nov	1561:1563	nov. (type strain TRE DT=DSM 103140T=JCM 31792T), Bifidobacterium felsineum sp.
30150092	4	102	dep	nov.	141:144	arg1	taxa					158:161	Four novel taxa	147:161	nov.: Four novel taxa isolated from the faeces of the cotton top tamarin (Saguinus oedipus) and the emperor tamarin (Saguinus imperator).	141:277	nov.: Four novel taxa isolated from the faeces of the cotton top tamarin (Saguinus oedipus) and the emperor tamarin (Saguinus imperator).
30150092	7	103	theme	%	857:857	arg1	values					833:838	similarity values	822:838	similarity values of 97.4% and 97.5%, respectively; TRI 7T	822:879	longum ATCC 15708T with similarity values of 97.4% and 97.5%, respectively; TRI 7T was closely related to Bifidobacterium tissieri DSM 100201 (96.0%).
30150092	7	104	with	15708T	810:815	arg1	values					833:838	similarity values	822:838	similarity values of 97.4% and 97.5%, respectively; TRI 7T	822:879	longum ATCC 15708T with similarity values of 97.4% and 97.5%, respectively; TRI 7T was closely related to Bifidobacterium tissieri DSM 100201 (96.0%).
30150092	11	105	theme	1T=DSM	1502:1507	arg1	nov					1480:1482	nov	1480:1482	nov	1480:1482	nov. (type strain TRE 1T=DSM 100687T=JCM 30945T), Bifidobacterium scaligerum sp.
30150092	11	105	theme	1T=DSM	1502:1507	arg1	30945T					1521:1526	type strain TRE 1T=DSM 100687T=JCM 30945T	1486:1526	type strain TRE 1T=DSM 100687T=JCM 30945T	1486:1526	nov. (type strain TRE 1T=DSM 100687T=JCM 30945T), Bifidobacterium scaligerum sp.
30150092	8	106	theme	closest	1027:1033	arg1	neighbour					1035:1043	closest neighbour	1027:1043	closest neighbour	1027:1043	The Average Nucleotide Identity (ANI) and in silico DDH (isDDH) analysis with closest neighbour supported an independent phylogenetic position of all strains with values ranged from 74 to 85% for ANI and from 24 to 28% for isDDH.
30150092	6	107	theme	longum	784:789	arg1	subsp					791:795	Bifidobacterium longum subsp	768:795	Bifidobacterium longum subsp	768:795	Phylogenetic analyses based on 16S rRNA revealed that bifidobacterial strains TRE 1T exhibit close phylogenetic relatedness to Bifidobacterium catulorum DSM 103154 (96.0%) and Bifidobacterium tissieri DSM 100201 (96.0%); TRE DT and TRE HT were closely related to Bifidobacterium longum subsp.
30150092	8	108	dep	28	1164:1165	arg1	to					1161:1162	to	1161:1162	to	1161:1162	The Average Nucleotide Identity (ANI) and in silico DDH (isDDH) analysis with closest neighbour supported an independent phylogenetic position of all strains with values ranged from 74 to 85% for ANI and from 24 to 28% for isDDH.
30150092	12	109	theme	type	1567:1570	arg1	31792T					1602:1607	type strain TRE DT=DSM 103140T=JCM 31792T	1567:1607	type strain TRE DT=DSM 103140T=JCM 31792T	1567:1607	nov. (type strain TRE DT=DSM 103140T=JCM 31792T), Bifidobacterium felsineum sp.
30150092	12	109	theme	type	1567:1570	arg1	nov					1561:1563	nov	1561:1563	nov	1561:1563	nov. (type strain TRE DT=DSM 103140T=JCM 31792T), Bifidobacterium felsineum sp.
30150092	10	110	dep	Bifidobacterium	1450:1464	arg1	primatium					1466:1474	primatium	1466:1474	primatium	1466:1474	Based on the phylogenetic, genotypic and phenotypic data, the strains TRE 1T, TRE DT, TRE HT and TRI 7T clearly represent four novel taxa within the genus Bifidobacterium for which the names Bifidobacterium primatium sp.
30150092	9	111	theme	%	1252:1252	arg1	G+C					1254:1256	58.3-63.5mol% G+C	1240:1256	58.3-63.5mol% G+C	1240:1256	DNA base composition of the four strains was in the range of 58.3-63.5mol% G+C.
30204584	11	0	theme	salmoninarum	1448:1459	arg1	ATCC					1461:1464	R. salmoninarum ATCC 33209T	1445:1471	R. salmoninarum ATCC 33209T	1445:1471	The DNA-DNA relatedness of strain IHBB 11108T was 46.7±2.2, 43.1±2.5 and 19.1±2.4 % with P. silvestre DSM 102047T, A. russicus DSM 14555T and R. salmoninarum ATCC 33209T, respectively.
30204584	5	1	theme	silvestre	591:599	arg1	%					619:619	97.5 %	614:619	97.5 %	614:619	The 16S rRNA gene sequence analysis revealed the highest relatedness of strain IHBB 11108T with Psychromicrobium silvestre DSM 102047T (97.5 %), Arthrobacter russicus DSM 14555T (97.4 %) and Renibacterium salmoninarum ATCC 33209T (97.4 %).
30204584	5	1	theme	silvestre	591:599	arg1	102047T					605:611	Psychromicrobium silvestre DSM 102047T	574:611	Psychromicrobium silvestre DSM 102047T (97.5 %)	574:620	The 16S rRNA gene sequence analysis revealed the highest relatedness of strain IHBB 11108T with Psychromicrobium silvestre DSM 102047T (97.5 %), Arthrobacter russicus DSM 14555T (97.4 %) and Renibacterium salmoninarum ATCC 33209T (97.4 %).
30204584	12	2	dep	results	1508:1514	arg1	basis					1495:1499	basis	1495:1499	basis	1495:1499	On the basis of the results of the phenotypic, chemotaxonomic and phylogenetic analyses, IHBB 11108T is considered to represent a novel species of the genus Psychromicrobium for which the name Psychromicrobium lacuslunae sp.
30204584	12	2	dep	results	1508:1514	arg1	the					1491:1493	the	1491:1493	the	1491:1493	On the basis of the results of the phenotypic, chemotaxonomic and phylogenetic analyses, IHBB 11108T is considered to represent a novel species of the genus Psychromicrobium for which the name Psychromicrobium lacuslunae sp.
30204584	12	3	dep	Psychromicrobium	1681:1696	arg1	lacuslunae					1698:1707	lacuslunae	1698:1707	lacuslunae	1698:1707	On the basis of the results of the phenotypic, chemotaxonomic and phylogenetic analyses, IHBB 11108T is considered to represent a novel species of the genus Psychromicrobium for which the name Psychromicrobium lacuslunae sp.
30204584	5	4	theme	IHBB	557:560	arg1	11108T					562:567	strain IHBB 11108T	550:567	strain IHBB 11108T	550:567	The 16S rRNA gene sequence analysis revealed the highest relatedness of strain IHBB 11108T with Psychromicrobium silvestre DSM 102047T (97.5 %), Arthrobacter russicus DSM 14555T (97.4 %) and Renibacterium salmoninarum ATCC 33209T (97.4 %).
30204584	11	5	theme	silvestre	1395:1403	arg1	102047T					1409:1415	P. silvestre DSM 102047T	1392:1415	P. silvestre DSM 102047T	1392:1415	The DNA-DNA relatedness of strain IHBB 11108T was 46.7±2.2, 43.1±2.5 and 19.1±2.4 % with P. silvestre DSM 102047T, A. russicus DSM 14555T and R. salmoninarum ATCC 33209T, respectively.
30204584	2	6	theme	Indian	249:254	arg1	trans-Himalayas					256:270	the Indian trans-Himalayas	245:270	the Indian trans-Himalayas	245:270	The novel strain IHBB 11108T was a psychrotolerant and alkaliphilic bacterium isolated from the subsurface water of Chandra Tal Lake in the Lahaul-Spiti valley located in the Indian trans-Himalayas.
30204584	12	7	theme	chemotaxonomic	1535:1548	arg1	analyses					1567:1574	the phenotypic, chemotaxonomic and phylogenetic analyses	1519:1574	the phenotypic, chemotaxonomic and phylogenetic analyses	1519:1574	On the basis of the results of the phenotypic, chemotaxonomic and phylogenetic analyses, IHBB 11108T is considered to represent a novel species of the genus Psychromicrobium for which the name Psychromicrobium lacuslunae sp.
30204584	8	8	theme	peptidoglycan	1053:1065	arg1	structure					1067:1075	The cell-wall peptidoglycan structure	1039:1075	The cell-wall peptidoglycan structure type	1039:1080	The cell-wall peptidoglycan structure type was A3α l-Lys-l-Thr-l-Ala with substitution of the α-carboxyl group of d-Glu by alanine amide.
30204584	5	9	dep	Renibacterium	669:681	arg1	salmoninarum					683:694	salmoninarum	683:694	salmoninarum	683:694	The 16S rRNA gene sequence analysis revealed the highest relatedness of strain IHBB 11108T with Psychromicrobium silvestre DSM 102047T (97.5 %), Arthrobacter russicus DSM 14555T (97.4 %) and Renibacterium salmoninarum ATCC 33209T (97.4 %).
30204584	12	10	theme	phylogenetic	1554:1565	arg1	analyses					1567:1574	the phenotypic, chemotaxonomic and phylogenetic analyses	1519:1574	the phenotypic, chemotaxonomic and phylogenetic analyses	1519:1574	On the basis of the results of the phenotypic, chemotaxonomic and phylogenetic analyses, IHBB 11108T is considered to represent a novel species of the genus Psychromicrobium for which the name Psychromicrobium lacuslunae sp.
30204584	12	11	theme	IHBB	1577:1580	arg1	11108T					1582:1587	IHBB 11108T	1577:1587	IHBB 11108T	1577:1587	On the basis of the results of the phenotypic, chemotaxonomic and phylogenetic analyses, IHBB 11108T is considered to represent a novel species of the genus Psychromicrobium for which the name Psychromicrobium lacuslunae sp.
30204584	8	12	theme	α-carboxyl	1133:1142	arg1	d-Glu					1153:1157	d-Glu	1153:1157	d-Glu	1153:1157	The cell-wall peptidoglycan structure type was A3α l-Lys-l-Thr-l-Ala with substitution of the α-carboxyl group of d-Glu by alanine amide.
30204584	8	12	theme	α-carboxyl	1133:1142	arg1	group					1144:1148	the α-carboxyl group	1129:1148	the α-carboxyl group of d-Glu	1129:1157	The cell-wall peptidoglycan structure type was A3α l-Lys-l-Thr-l-Ala with substitution of the α-carboxyl group of d-Glu by alanine amide.
30204584	5	13	theme	Arthrobacter	623:634	arg1	14555T					649:654	Arthrobacter russicus DSM 14555T	623:654	Arthrobacter russicus DSM 14555T (97.4 %)	623:663	The 16S rRNA gene sequence analysis revealed the highest relatedness of strain IHBB 11108T with Psychromicrobium silvestre DSM 102047T (97.5 %), Arthrobacter russicus DSM 14555T (97.4 %) and Renibacterium salmoninarum ATCC 33209T (97.4 %).
30204584	5	13	theme	Arthrobacter	623:634	arg1	%					662:662	97.4 %	657:662	97.4 %	657:662	The 16S rRNA gene sequence analysis revealed the highest relatedness of strain IHBB 11108T with Psychromicrobium silvestre DSM 102047T (97.5 %), Arthrobacter russicus DSM 14555T (97.4 %) and Renibacterium salmoninarum ATCC 33209T (97.4 %).
30204584	4	14	theme	optimum	423:429	arg1	pH					431:432	optimum pH 7.0	423:436	optimum pH 7.0	423:436	The strain grew at 5-37 °C (optimum 28 °C), pH 5.0-12.0 (optimum pH 7.0) and with up to 8 % NaCl (optimum 1 %).
30204584	4	14	theme	optimum	423:429	arg1	pH					410:411	pH 5.0-12.0	410:420	pH 5.0-12.0 (optimum pH 7.0)	410:437	The strain grew at 5-37 °C (optimum 28 °C), pH 5.0-12.0 (optimum pH 7.0) and with up to 8 % NaCl (optimum 1 %).
30204584	2	15	theme	strain	84:89	arg1	11108T					96:101	The novel strain IHBB 11108T	74:101	The novel strain IHBB 11108T	74:101	The novel strain IHBB 11108T was a psychrotolerant and alkaliphilic bacterium isolated from the subsurface water of Chandra Tal Lake in the Lahaul-Spiti valley located in the Indian trans-Himalayas.
30204584	2	15	theme	strain	84:89	arg1	bacterium					142:150	a psychrotolerant and alkaliphilic bacterium	107:150	a psychrotolerant and alkaliphilic bacterium isolated from the subsurface water of Chandra Tal Lake	107:205	The novel strain IHBB 11108T was a psychrotolerant and alkaliphilic bacterium isolated from the subsurface water of Chandra Tal Lake in the Lahaul-Spiti valley located in the Indian trans-Himalayas.
30204584	5	16	theme	DSM	645:647	arg1	14555T					649:654	Arthrobacter russicus DSM 14555T	623:654	Arthrobacter russicus DSM 14555T (97.4 %)	623:663	The 16S rRNA gene sequence analysis revealed the highest relatedness of strain IHBB 11108T with Psychromicrobium silvestre DSM 102047T (97.5 %), Arthrobacter russicus DSM 14555T (97.4 %) and Renibacterium salmoninarum ATCC 33209T (97.4 %).
30204584	5	16	theme	DSM	645:647	arg1	%					662:662	97.4 %	657:662	97.4 %	657:662	The 16S rRNA gene sequence analysis revealed the highest relatedness of strain IHBB 11108T with Psychromicrobium silvestre DSM 102047T (97.5 %), Arthrobacter russicus DSM 14555T (97.4 %) and Renibacterium salmoninarum ATCC 33209T (97.4 %).
30204584	2	17	from	bacterium	142:150	arg1	valley					227:232	the Lahaul-Spiti valley	210:232	the Lahaul-Spiti valley located in the Indian trans-Himalayas	210:270	The novel strain IHBB 11108T was a psychrotolerant and alkaliphilic bacterium isolated from the subsurface water of Chandra Tal Lake in the Lahaul-Spiti valley located in the Indian trans-Himalayas.
30204584	8	18	with	l-Lys-l-Thr-l-Ala	1090:1106	arg1	substitution					1113:1124	substitution	1113:1124	substitution of the α-carboxyl group of d-Glu by alanine amide	1113:1174	The cell-wall peptidoglycan structure type was A3α l-Lys-l-Thr-l-Ala with substitution of the α-carboxyl group of d-Glu by alanine amide.
30204584	7	19	theme	polar	835:839	arg1	lipids					841:846	The polar lipids	831:846	The polar lipids detected	831:855	The polar lipids detected were diphosphatidylglycerol, dimannosylglyceride, phosphatidylinositol, phosphatidylglycerol, monogalactosyldiacylglycerol, one unidentified glycolipid and four unidentified lipids.
30204584	7	19	theme	polar	835:839	arg1	diphosphatidylglycerol					862:883	diphosphatidylglycerol	862:883	diphosphatidylglycerol	862:883	The polar lipids detected were diphosphatidylglycerol, dimannosylglyceride, phosphatidylinositol, phosphatidylglycerol, monogalactosyldiacylglycerol, one unidentified glycolipid and four unidentified lipids.
30204584	2	20	theme	Lake	202:205	arg1	water					181:185	the subsurface water	166:185	the subsurface water of Chandra Tal Lake	166:205	The novel strain IHBB 11108T was a psychrotolerant and alkaliphilic bacterium isolated from the subsurface water of Chandra Tal Lake in the Lahaul-Spiti valley located in the Indian trans-Himalayas.
30204584	5	21	theme	16S	482:484	arg1	rRNA					486:489	The 16S rRNA	478:489	The 16S rRNA gene sequence analysis	478:512	The 16S rRNA gene sequence analysis revealed the highest relatedness of strain IHBB 11108T with Psychromicrobium silvestre DSM 102047T (97.5 %), Arthrobacter russicus DSM 14555T (97.4 %) and Renibacterium salmoninarum ATCC 33209T (97.4 %).
30204584	2	22	from	trans-Himalayas	256:270	arg1	located					234:240	located	234:240	located	234:240	The novel strain IHBB 11108T was a psychrotolerant and alkaliphilic bacterium isolated from the subsurface water of Chandra Tal Lake in the Lahaul-Spiti valley located in the Indian trans-Himalayas.
30204584	11	23	theme	IHBB	1337:1340	arg1	11108T					1342:1347	strain IHBB 11108T	1330:1347	strain IHBB 11108T	1330:1347	The DNA-DNA relatedness of strain IHBB 11108T was 46.7±2.2, 43.1±2.5 and 19.1±2.4 % with P. silvestre DSM 102047T, A. russicus DSM 14555T and R. salmoninarum ATCC 33209T, respectively.
30204584	10	24	theme	59.0 mol	1292:1299	arg1	G+C content					1276:1286	The genomic DNA G+C content	1260:1286	The genomic DNA G+C content	1260:1286	The genomic DNA G+C content was 59.0 mol%.
30204584	10	24	theme	59.0 mol	1292:1299	arg1	%					1300:1300	59.0 mol%	1292:1300	59.0 mol%	1292:1300	The genomic DNA G+C content was 59.0 mol%.
30204584	1	25	theme	high	54:57	arg1	lake					68:71	a high altitude lake	52:71	a high altitude lake	52:71	nov., isolated from a high altitude lake.
30204584	5	26	theme	ATCC	696:699	arg1	33209T					701:706	Renibacterium salmoninarum ATCC 33209T	669:706	Renibacterium salmoninarum ATCC 33209T (97.4 %)	669:715	The 16S rRNA gene sequence analysis revealed the highest relatedness of strain IHBB 11108T with Psychromicrobium silvestre DSM 102047T (97.5 %), Arthrobacter russicus DSM 14555T (97.4 %) and Renibacterium salmoninarum ATCC 33209T (97.4 %).
30204584	5	26	theme	ATCC	696:699	arg1	%					714:714	97.4 %	709:714	97.4 %	709:714	The 16S rRNA gene sequence analysis revealed the highest relatedness of strain IHBB 11108T with Psychromicrobium silvestre DSM 102047T (97.5 %), Arthrobacter russicus DSM 14555T (97.4 %) and Renibacterium salmoninarum ATCC 33209T (97.4 %).
30204584	5	27	theme	gene	491:494	arg1	analysis					505:512	The 16S rRNA gene sequence analysis	478:512	The 16S rRNA gene sequence analysis	478:512	The 16S rRNA gene sequence analysis revealed the highest relatedness of strain IHBB 11108T with Psychromicrobium silvestre DSM 102047T (97.5 %), Arthrobacter russicus DSM 14555T (97.4 %) and Renibacterium salmoninarum ATCC 33209T (97.4 %).
30204584	0	28	theme	Psychromicrobium	0:15	arg1	sp					28:29	Psychromicrobium lacuslunae sp	0:29	Psychromicrobium lacuslunae sp.	0:30	Psychromicrobium lacuslunae sp.
30204584	2	29	from	located	234:240	arg1	trans-Himalayas					256:270	the Indian trans-Himalayas	245:270	the Indian trans-Himalayas	245:270	The novel strain IHBB 11108T was a psychrotolerant and alkaliphilic bacterium isolated from the subsurface water of Chandra Tal Lake in the Lahaul-Spiti valley located in the Indian trans-Himalayas.
30204584	11	30	theme	DNA-DNA	1307:1313	arg1	relatedness					1315:1325	The DNA-DNA relatedness	1303:1325	The DNA-DNA relatedness of strain IHBB 11108T	1303:1347	The DNA-DNA relatedness of strain IHBB 11108T was 46.7±2.2, 43.1±2.5 and 19.1±2.4 % with P. silvestre DSM 102047T, A. russicus DSM 14555T and R. salmoninarum ATCC 33209T, respectively.
30204584	11	30	theme	DNA-DNA	1307:1313	arg1	%					1385:1385	46.7±2.2, 43.1±2.5 and 19.1±2.4 %	1353:1385	46.7±2.2, 43.1±2.5 and 19.1±2.4 % with P. silvestre DSM 102047T	1353:1415	The DNA-DNA relatedness of strain IHBB 11108T was 46.7±2.2, 43.1±2.5 and 19.1±2.4 % with P. silvestre DSM 102047T, A. russicus DSM 14555T and R. salmoninarum ATCC 33209T, respectively.
30204584	14	31	theme	31143T=KACC	1790:1800	arg1	11108T					1755:1760	IHBB 11108T	1750:1760	IHBB 11108T (=MTCC 12460T=MCC 2780T=JCM 31143T=KACC 19070T)	1750:1808	The type strain is IHBB 11108T (=MTCC 12460T=MCC 2780T=JCM 31143T=KACC 19070T).
30204584	14	31	theme	31143T=KACC	1790:1800	arg1	19070T					1802:1807	=MTCC 12460T=MCC 2780T=JCM 31143T=KACC 19070T	1763:1807	=MTCC 12460T=MCC 2780T=JCM 31143T=KACC 19070T	1763:1807	The type strain is IHBB 11108T (=MTCC 12460T=MCC 2780T=JCM 31143T=KACC 19070T).
30204584	12	32	theme	Psychromicrobium	1681:1696	arg1	sp					1709:1710	the name Psychromicrobium lacuslunae sp	1672:1710	the name Psychromicrobium lacuslunae sp	1672:1710	On the basis of the results of the phenotypic, chemotaxonomic and phylogenetic analyses, IHBB 11108T is considered to represent a novel species of the genus Psychromicrobium for which the name Psychromicrobium lacuslunae sp.
30204584	14	33	theme	12460T=MCC	1769:1778	arg1	11108T					1755:1760	IHBB 11108T	1750:1760	IHBB 11108T (=MTCC 12460T=MCC 2780T=JCM 31143T=KACC 19070T)	1750:1808	The type strain is IHBB 11108T (=MTCC 12460T=MCC 2780T=JCM 31143T=KACC 19070T).
30204584	14	33	theme	12460T=MCC	1769:1778	arg1	19070T					1802:1807	=MTCC 12460T=MCC 2780T=JCM 31143T=KACC 19070T	1763:1807	=MTCC 12460T=MCC 2780T=JCM 31143T=KACC 19070T	1763:1807	The type strain is IHBB 11108T (=MTCC 12460T=MCC 2780T=JCM 31143T=KACC 19070T).
30204584	4	34	theme	optimum	464:470	arg1	%					474:474	optimum 1 %	464:474	optimum 1 %	464:474	The strain grew at 5-37 °C (optimum 28 °C), pH 5.0-12.0 (optimum pH 7.0) and with up to 8 % NaCl (optimum 1 %).
30204584	4	34	theme	optimum	464:470	arg1	NaCl					458:461	up to 8 % NaCl	448:461	up to 8 % NaCl (optimum 1 %)	448:475	The strain grew at 5-37 °C (optimum 28 °C), pH 5.0-12.0 (optimum pH 7.0) and with up to 8 % NaCl (optimum 1 %).
30204584	4	35	dep	%	456:456	arg1	8 					454:455	8 	454:455	8 	454:455	The strain grew at 5-37 °C (optimum 28 °C), pH 5.0-12.0 (optimum pH 7.0) and with up to 8 % NaCl (optimum 1 %).
30204584	12	36	theme	genus	1639:1643	arg1	Psychromicrobium					1645:1660	the genus Psychromicrobium	1635:1660	the genus Psychromicrobium	1635:1660	On the basis of the results of the phenotypic, chemotaxonomic and phylogenetic analyses, IHBB 11108T is considered to represent a novel species of the genus Psychromicrobium for which the name Psychromicrobium lacuslunae sp.
30204584	6	37	theme	quinone	741:747	arg1	system					749:754	a quinone system	739:754	a quinone system	739:754	The strain contained a quinone system with 57.2 % MK-9(H2), 39.1 % MK-10(H2), 3.0 % MK-8(H2) and 0.7 % MK-7(H2).
30204584	11	38	with	%	1385:1385	arg1	102047T					1409:1415	P. silvestre DSM 102047T	1392:1415	P. silvestre DSM 102047T	1392:1415	The DNA-DNA relatedness of strain IHBB 11108T was 46.7±2.2, 43.1±2.5 and 19.1±2.4 % with P. silvestre DSM 102047T, A. russicus DSM 14555T and R. salmoninarum ATCC 33209T, respectively.
30204584	10	39	theme	genomic	1264:1270	arg1	G+C content					1276:1286	The genomic DNA G+C content	1260:1286	The genomic DNA G+C content	1260:1286	The genomic DNA G+C content was 59.0 mol%.
30204584	10	39	theme	genomic	1264:1270	arg1	%					1300:1300	59.0 mol%	1292:1300	59.0 mol%	1292:1300	The genomic DNA G+C content was 59.0 mol%.
30204584	14	40	theme	IHBB	1750:1753	arg1	strain					1740:1745	The type strain	1731:1745	The type strain	1731:1745	The type strain is IHBB 11108T (=MTCC 12460T=MCC 2780T=JCM 31143T=KACC 19070T).
30204584	14	40	theme	IHBB	1750:1753	arg1	11108T					1755:1760	IHBB 11108T	1750:1760	IHBB 11108T (=MTCC 12460T=MCC 2780T=JCM 31143T=KACC 19070T)	1750:1808	The type strain is IHBB 11108T (=MTCC 12460T=MCC 2780T=JCM 31143T=KACC 19070T).
30204584	14	40	theme	IHBB	1750:1753	arg1	19070T					1802:1807	=MTCC 12460T=MCC 2780T=JCM 31143T=KACC 19070T	1763:1807	=MTCC 12460T=MCC 2780T=JCM 31143T=KACC 19070T	1763:1807	The type strain is IHBB 11108T (=MTCC 12460T=MCC 2780T=JCM 31143T=KACC 19070T).
30204584	11	41	with	14555T	1434:1439	arg1	102047T					1409:1415	P. silvestre DSM 102047T	1392:1415	P. silvestre DSM 102047T	1392:1415	The DNA-DNA relatedness of strain IHBB 11108T was 46.7±2.2, 43.1±2.5 and 19.1±2.4 % with P. silvestre DSM 102047T, A. russicus DSM 14555T and R. salmoninarum ATCC 33209T, respectively.
30204584	11	42	theme	R.	1445:1446	arg1	ATCC					1461:1464	R. salmoninarum ATCC 33209T	1445:1471	R. salmoninarum ATCC 33209T	1445:1471	The DNA-DNA relatedness of strain IHBB 11108T was 46.7±2.2, 43.1±2.5 and 19.1±2.4 % with P. silvestre DSM 102047T, A. russicus DSM 14555T and R. salmoninarum ATCC 33209T, respectively.
30204584	9	43	theme	predominant	1235:1245	arg1	Anteiso-C15 					1177:1188	Anteiso-C15 	1177:1188	Anteiso-C15 	1177:1188	Anteiso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0 were the predominant fatty acids.
30204584	9	43	theme	predominant	1235:1245	arg1	acids					1253:1257	the predominant fatty acids	1231:1257	the predominant fatty acids	1231:1257	Anteiso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0 were the predominant fatty acids.
30204584	5	44	theme	Psychromicrobium	574:589	arg1	%					619:619	97.5 %	614:619	97.5 %	614:619	The 16S rRNA gene sequence analysis revealed the highest relatedness of strain IHBB 11108T with Psychromicrobium silvestre DSM 102047T (97.5 %), Arthrobacter russicus DSM 14555T (97.4 %) and Renibacterium salmoninarum ATCC 33209T (97.4 %).
30204584	5	44	theme	Psychromicrobium	574:589	arg1	102047T					605:611	Psychromicrobium silvestre DSM 102047T	574:611	Psychromicrobium silvestre DSM 102047T (97.5 %)	574:620	The 16S rRNA gene sequence analysis revealed the highest relatedness of strain IHBB 11108T with Psychromicrobium silvestre DSM 102047T (97.5 %), Arthrobacter russicus DSM 14555T (97.4 %) and Renibacterium salmoninarum ATCC 33209T (97.4 %).
30204584	11	45	with	ATCC	1461:1464	arg1	102047T					1409:1415	P. silvestre DSM 102047T	1392:1415	P. silvestre DSM 102047T	1392:1415	The DNA-DNA relatedness of strain IHBB 11108T was 46.7±2.2, 43.1±2.5 and 19.1±2.4 % with P. silvestre DSM 102047T, A. russicus DSM 14555T and R. salmoninarum ATCC 33209T, respectively.
30204584	4	46	theme	optimum	394:400	arg1	5-37 °C					385:391	5-37 °C	385:391	5-37 °C (optimum 28 °C)	385:407	The strain grew at 5-37 °C (optimum 28 °C), pH 5.0-12.0 (optimum pH 7.0) and with up to 8 % NaCl (optimum 1 %).
30204584	4	46	theme	optimum	394:400	arg1	28 °C					402:406	optimum 28 °C	394:406	optimum 28 °C	394:406	The strain grew at 5-37 °C (optimum 28 °C), pH 5.0-12.0 (optimum pH 7.0) and with up to 8 % NaCl (optimum 1 %).
30204584	2	47	theme	IHBB	91:94	arg1	11108T					96:101	The novel strain IHBB 11108T	74:101	The novel strain IHBB 11108T	74:101	The novel strain IHBB 11108T was a psychrotolerant and alkaliphilic bacterium isolated from the subsurface water of Chandra Tal Lake in the Lahaul-Spiti valley located in the Indian trans-Himalayas.
30204584	2	47	theme	IHBB	91:94	arg1	bacterium					142:150	a psychrotolerant and alkaliphilic bacterium	107:150	a psychrotolerant and alkaliphilic bacterium isolated from the subsurface water of Chandra Tal Lake	107:205	The novel strain IHBB 11108T was a psychrotolerant and alkaliphilic bacterium isolated from the subsurface water of Chandra Tal Lake in the Lahaul-Spiti valley located in the Indian trans-Himalayas.
30204584	5	48	theme	DSM	601:603	arg1	%					619:619	97.5 %	614:619	97.5 %	614:619	The 16S rRNA gene sequence analysis revealed the highest relatedness of strain IHBB 11108T with Psychromicrobium silvestre DSM 102047T (97.5 %), Arthrobacter russicus DSM 14555T (97.4 %) and Renibacterium salmoninarum ATCC 33209T (97.4 %).
30204584	5	48	theme	DSM	601:603	arg1	102047T					605:611	Psychromicrobium silvestre DSM 102047T	574:611	Psychromicrobium silvestre DSM 102047T (97.5 %)	574:620	The 16S rRNA gene sequence analysis revealed the highest relatedness of strain IHBB 11108T with Psychromicrobium silvestre DSM 102047T (97.5 %), Arthrobacter russicus DSM 14555T (97.4 %) and Renibacterium salmoninarum ATCC 33209T (97.4 %).
30204584	2	49	theme	alkaliphilic	129:140	arg1	11108T					96:101	The novel strain IHBB 11108T	74:101	The novel strain IHBB 11108T	74:101	The novel strain IHBB 11108T was a psychrotolerant and alkaliphilic bacterium isolated from the subsurface water of Chandra Tal Lake in the Lahaul-Spiti valley located in the Indian trans-Himalayas.
30204584	2	49	theme	alkaliphilic	129:140	arg1	bacterium					142:150	a psychrotolerant and alkaliphilic bacterium	107:150	a psychrotolerant and alkaliphilic bacterium isolated from the subsurface water of Chandra Tal Lake	107:205	The novel strain IHBB 11108T was a psychrotolerant and alkaliphilic bacterium isolated from the subsurface water of Chandra Tal Lake in the Lahaul-Spiti valley located in the Indian trans-Himalayas.
30204584	5	50	theme	strain	550:555	arg1	11108T					562:567	strain IHBB 11108T	550:567	strain IHBB 11108T	550:567	The 16S rRNA gene sequence analysis revealed the highest relatedness of strain IHBB 11108T with Psychromicrobium silvestre DSM 102047T (97.5 %), Arthrobacter russicus DSM 14555T (97.4 %) and Renibacterium salmoninarum ATCC 33209T (97.4 %).
30204584	14	51	theme	type	1735:1738	arg1	strain					1740:1745	The type strain	1731:1745	The type strain	1731:1745	The type strain is IHBB 11108T (=MTCC 12460T=MCC 2780T=JCM 31143T=KACC 19070T).
30204584	14	51	theme	type	1735:1738	arg1	11108T					1755:1760	IHBB 11108T	1750:1760	IHBB 11108T (=MTCC 12460T=MCC 2780T=JCM 31143T=KACC 19070T)	1750:1808	The type strain is IHBB 11108T (=MTCC 12460T=MCC 2780T=JCM 31143T=KACC 19070T).
30204584	11	52	dep	A.	1418:1419	arg1	russicus					1421:1428	russicus	1421:1428	russicus	1421:1428	The DNA-DNA relatedness of strain IHBB 11108T was 46.7±2.2, 43.1±2.5 and 19.1±2.4 % with P. silvestre DSM 102047T, A. russicus DSM 14555T and R. salmoninarum ATCC 33209T, respectively.
30204584	2	53	theme	psychrotolerant	109:123	arg1	11108T					96:101	The novel strain IHBB 11108T	74:101	The novel strain IHBB 11108T	74:101	The novel strain IHBB 11108T was a psychrotolerant and alkaliphilic bacterium isolated from the subsurface water of Chandra Tal Lake in the Lahaul-Spiti valley located in the Indian trans-Himalayas.
30204584	2	53	theme	psychrotolerant	109:123	arg1	bacterium					142:150	a psychrotolerant and alkaliphilic bacterium	107:150	a psychrotolerant and alkaliphilic bacterium isolated from the subsurface water of Chandra Tal Lake	107:205	The novel strain IHBB 11108T was a psychrotolerant and alkaliphilic bacterium isolated from the subsurface water of Chandra Tal Lake in the Lahaul-Spiti valley located in the Indian trans-Himalayas.
30204584	5	54	theme	11108T	562:567	arg1	relatedness					535:545	the highest relatedness	523:545	the highest relatedness of strain IHBB 11108T with Psychromicrobium silvestre DSM 102047T (97.5 %), Arthrobacter russicus DSM 14555T (97.4 %) and Renibacterium salmoninarum ATCC 33209T (97.4 %)	523:715	The 16S rRNA gene sequence analysis revealed the highest relatedness of strain IHBB 11108T with Psychromicrobium silvestre DSM 102047T (97.5 %), Arthrobacter russicus DSM 14555T (97.4 %) and Renibacterium salmoninarum ATCC 33209T (97.4 %).
30204584	11	55	theme	DSM	1405:1407	arg1	102047T					1409:1415	P. silvestre DSM 102047T	1392:1415	P. silvestre DSM 102047T	1392:1415	The DNA-DNA relatedness of strain IHBB 11108T was 46.7±2.2, 43.1±2.5 and 19.1±2.4 % with P. silvestre DSM 102047T, A. russicus DSM 14555T and R. salmoninarum ATCC 33209T, respectively.
30204584	12	56	theme	analyses	1567:1574	arg1	results					1508:1514	the results	1504:1514	the results of the phenotypic, chemotaxonomic and phylogenetic analyses	1504:1574	On the basis of the results of the phenotypic, chemotaxonomic and phylogenetic analyses, IHBB 11108T is considered to represent a novel species of the genus Psychromicrobium for which the name Psychromicrobium lacuslunae sp.
30204584	11	57	theme	P.	1392:1393	arg1	102047T					1409:1415	P. silvestre DSM 102047T	1392:1415	P. silvestre DSM 102047T	1392:1415	The DNA-DNA relatedness of strain IHBB 11108T was 46.7±2.2, 43.1±2.5 and 19.1±2.4 % with P. silvestre DSM 102047T, A. russicus DSM 14555T and R. salmoninarum ATCC 33209T, respectively.
30204584	8	58	theme	cell-wall	1043:1051	arg1	structure					1067:1075	The cell-wall peptidoglycan structure	1039:1075	The cell-wall peptidoglycan structure type	1039:1080	The cell-wall peptidoglycan structure type was A3α l-Lys-l-Thr-l-Ala with substitution of the α-carboxyl group of d-Glu by alanine amide.
30204584	2	59	theme	Lahaul-Spiti	214:225	arg1	valley					227:232	the Lahaul-Spiti valley	210:232	the Lahaul-Spiti valley located in the Indian trans-Himalayas	210:270	The novel strain IHBB 11108T was a psychrotolerant and alkaliphilic bacterium isolated from the subsurface water of Chandra Tal Lake in the Lahaul-Spiti valley located in the Indian trans-Himalayas.
30204584	8	60	theme	structure	1067:1075	arg1	type					1077:1080	The cell-wall peptidoglycan structure type	1039:1080	The cell-wall peptidoglycan structure type	1039:1080	The cell-wall peptidoglycan structure type was A3α l-Lys-l-Thr-l-Ala with substitution of the α-carboxyl group of d-Glu by alanine amide.
30204584	5	61	with	relatedness	535:545	arg1	%					714:714	97.4 %	709:714	97.4 %	709:714	The 16S rRNA gene sequence analysis revealed the highest relatedness of strain IHBB 11108T with Psychromicrobium silvestre DSM 102047T (97.5 %), Arthrobacter russicus DSM 14555T (97.4 %) and Renibacterium salmoninarum ATCC 33209T (97.4 %).
30204584	5	61	with	relatedness	535:545	arg1	%					619:619	97.5 %	614:619	97.5 %	614:619	The 16S rRNA gene sequence analysis revealed the highest relatedness of strain IHBB 11108T with Psychromicrobium silvestre DSM 102047T (97.5 %), Arthrobacter russicus DSM 14555T (97.4 %) and Renibacterium salmoninarum ATCC 33209T (97.4 %).
30204584	5	61	with	relatedness	535:545	arg1	14555T					649:654	Arthrobacter russicus DSM 14555T	623:654	Arthrobacter russicus DSM 14555T (97.4 %)	623:663	The 16S rRNA gene sequence analysis revealed the highest relatedness of strain IHBB 11108T with Psychromicrobium silvestre DSM 102047T (97.5 %), Arthrobacter russicus DSM 14555T (97.4 %) and Renibacterium salmoninarum ATCC 33209T (97.4 %).
30204584	5	61	with	relatedness	535:545	arg1	%					662:662	97.4 %	657:662	97.4 %	657:662	The 16S rRNA gene sequence analysis revealed the highest relatedness of strain IHBB 11108T with Psychromicrobium silvestre DSM 102047T (97.5 %), Arthrobacter russicus DSM 14555T (97.4 %) and Renibacterium salmoninarum ATCC 33209T (97.4 %).
30204584	5	61	with	relatedness	535:545	arg1	33209T					701:706	Renibacterium salmoninarum ATCC 33209T	669:706	Renibacterium salmoninarum ATCC 33209T (97.4 %)	669:715	The 16S rRNA gene sequence analysis revealed the highest relatedness of strain IHBB 11108T with Psychromicrobium silvestre DSM 102047T (97.5 %), Arthrobacter russicus DSM 14555T (97.4 %) and Renibacterium salmoninarum ATCC 33209T (97.4 %).
30204584	5	61	with	relatedness	535:545	arg1	102047T					605:611	Psychromicrobium silvestre DSM 102047T	574:611	Psychromicrobium silvestre DSM 102047T (97.5 %)	574:620	The 16S rRNA gene sequence analysis revealed the highest relatedness of strain IHBB 11108T with Psychromicrobium silvestre DSM 102047T (97.5 %), Arthrobacter russicus DSM 14555T (97.4 %) and Renibacterium salmoninarum ATCC 33209T (97.4 %).
30204584	9	62	dep	Anteiso-C15 	1177:1188	arg1	anteiso-C17 					1210:1221	anteiso-C17 	1210:1221	anteiso-C17 	1210:1221	Anteiso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0 were the predominant fatty acids.
30204584	9	62	dep	Anteiso-C15 	1177:1188	arg1	 0					1203:1204	 0	1203:1204	 0	1203:1204	Anteiso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0 were the predominant fatty acids.
30204584	9	62	dep	Anteiso-C15 	1177:1188	arg1	 0					1223:1224	 0	1223:1224	 0	1223:1224	Anteiso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0 were the predominant fatty acids.
30204584	9	62	dep	Anteiso-C15 	1177:1188	arg1	 0					1190:1191	 0	1190:1191	 0	1190:1191	Anteiso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0 were the predominant fatty acids.
30204584	2	63	theme	novel	78:82	arg1	11108T					96:101	The novel strain IHBB 11108T	74:101	The novel strain IHBB 11108T	74:101	The novel strain IHBB 11108T was a psychrotolerant and alkaliphilic bacterium isolated from the subsurface water of Chandra Tal Lake in the Lahaul-Spiti valley located in the Indian trans-Himalayas.
30204584	2	63	theme	novel	78:82	arg1	bacterium					142:150	a psychrotolerant and alkaliphilic bacterium	107:150	a psychrotolerant and alkaliphilic bacterium isolated from the subsurface water of Chandra Tal Lake	107:205	The novel strain IHBB 11108T was a psychrotolerant and alkaliphilic bacterium isolated from the subsurface water of Chandra Tal Lake in the Lahaul-Spiti valley located in the Indian trans-Himalayas.
30204584	8	64	theme	d-Glu	1153:1157	arg1	d-Glu					1153:1157	d-Glu	1153:1157	d-Glu	1153:1157	The cell-wall peptidoglycan structure type was A3α l-Lys-l-Thr-l-Ala with substitution of the α-carboxyl group of d-Glu by alanine amide.
30204584	8	64	theme	d-Glu	1153:1157	arg1	group					1144:1148	the α-carboxyl group	1129:1148	the α-carboxyl group of d-Glu	1129:1157	The cell-wall peptidoglycan structure type was A3α l-Lys-l-Thr-l-Ala with substitution of the α-carboxyl group of d-Glu by alanine amide.
30204584	11	65	theme	strain	1330:1335	arg1	11108T					1342:1347	strain IHBB 11108T	1330:1347	strain IHBB 11108T	1330:1347	The DNA-DNA relatedness of strain IHBB 11108T was 46.7±2.2, 43.1±2.5 and 19.1±2.4 % with P. silvestre DSM 102047T, A. russicus DSM 14555T and R. salmoninarum ATCC 33209T, respectively.
30204584	5	66	theme	rRNA	486:489	arg1	analysis					505:512	The 16S rRNA gene sequence analysis	478:512	The 16S rRNA gene sequence analysis	478:512	The 16S rRNA gene sequence analysis revealed the highest relatedness of strain IHBB 11108T with Psychromicrobium silvestre DSM 102047T (97.5 %), Arthrobacter russicus DSM 14555T (97.4 %) and Renibacterium salmoninarum ATCC 33209T (97.4 %).
30204584	8	67	theme	alanine	1162:1168	arg1	amide					1170:1174	alanine amide	1162:1174	alanine amide	1162:1174	The cell-wall peptidoglycan structure type was A3α l-Lys-l-Thr-l-Ala with substitution of the α-carboxyl group of d-Glu by alanine amide.
30204584	2	68	theme	located	234:240	arg1	valley					227:232	the Lahaul-Spiti valley	210:232	the Lahaul-Spiti valley located in the Indian trans-Himalayas	210:270	The novel strain IHBB 11108T was a psychrotolerant and alkaliphilic bacterium isolated from the subsurface water of Chandra Tal Lake in the Lahaul-Spiti valley located in the Indian trans-Himalayas.
30204584	12	69	theme	phenotypic	1523:1532	arg1	analyses					1567:1574	the phenotypic, chemotaxonomic and phylogenetic analyses	1519:1574	the phenotypic, chemotaxonomic and phylogenetic analyses	1519:1574	On the basis of the results of the phenotypic, chemotaxonomic and phylogenetic analyses, IHBB 11108T is considered to represent a novel species of the genus Psychromicrobium for which the name Psychromicrobium lacuslunae sp.
30204584	6	70	contain	contained	729:737	arg1	strain					722:727	The strain	718:727	The strain	718:727	The strain contained a quinone system with 57.2 % MK-9(H2), 39.1 % MK-10(H2), 3.0 % MK-8(H2) and 0.7 % MK-7(H2).
30204584	6	70	contain	contained	729:737	arg2	system					749:754	a quinone system	739:754	a quinone system	739:754	The strain contained a quinone system with 57.2 % MK-9(H2), 39.1 % MK-10(H2), 3.0 % MK-8(H2) and 0.7 % MK-7(H2).
30204584	5	71	theme	Renibacterium	669:681	arg1	33209T					701:706	Renibacterium salmoninarum ATCC 33209T	669:706	Renibacterium salmoninarum ATCC 33209T (97.4 %)	669:715	The 16S rRNA gene sequence analysis revealed the highest relatedness of strain IHBB 11108T with Psychromicrobium silvestre DSM 102047T (97.5 %), Arthrobacter russicus DSM 14555T (97.4 %) and Renibacterium salmoninarum ATCC 33209T (97.4 %).
30204584	5	71	theme	Renibacterium	669:681	arg1	%					714:714	97.4 %	709:714	97.4 %	709:714	The 16S rRNA gene sequence analysis revealed the highest relatedness of strain IHBB 11108T with Psychromicrobium silvestre DSM 102047T (97.5 %), Arthrobacter russicus DSM 14555T (97.4 %) and Renibacterium salmoninarum ATCC 33209T (97.4 %).
30204584	5	72	dep	Arthrobacter	623:634	arg1	russicus					636:643	russicus	636:643	russicus	636:643	The 16S rRNA gene sequence analysis revealed the highest relatedness of strain IHBB 11108T with Psychromicrobium silvestre DSM 102047T (97.5 %), Arthrobacter russicus DSM 14555T (97.4 %) and Renibacterium salmoninarum ATCC 33209T (97.4 %).
30204584	11	73	theme	11108T	1342:1347	arg1	relatedness					1315:1325	The DNA-DNA relatedness	1303:1325	The DNA-DNA relatedness of strain IHBB 11108T	1303:1347	The DNA-DNA relatedness of strain IHBB 11108T was 46.7±2.2, 43.1±2.5 and 19.1±2.4 % with P. silvestre DSM 102047T, A. russicus DSM 14555T and R. salmoninarum ATCC 33209T, respectively.
30204584	11	73	theme	11108T	1342:1347	arg1	%					1385:1385	46.7±2.2, 43.1±2.5 and 19.1±2.4 %	1353:1385	46.7±2.2, 43.1±2.5 and 19.1±2.4 % with P. silvestre DSM 102047T	1353:1415	The DNA-DNA relatedness of strain IHBB 11108T was 46.7±2.2, 43.1±2.5 and 19.1±2.4 % with P. silvestre DSM 102047T, A. russicus DSM 14555T and R. salmoninarum ATCC 33209T, respectively.
30204584	5	74	theme	highest	527:533	arg1	relatedness					535:545	the highest relatedness	523:545	the highest relatedness of strain IHBB 11108T with Psychromicrobium silvestre DSM 102047T (97.5 %), Arthrobacter russicus DSM 14555T (97.4 %) and Renibacterium salmoninarum ATCC 33209T (97.4 %)	523:715	The 16S rRNA gene sequence analysis revealed the highest relatedness of strain IHBB 11108T with Psychromicrobium silvestre DSM 102047T (97.5 %), Arthrobacter russicus DSM 14555T (97.4 %) and Renibacterium salmoninarum ATCC 33209T (97.4 %).
30204584	10	75	theme	DNA	1272:1274	arg1	G+C content					1276:1286	The genomic DNA G+C content	1260:1286	The genomic DNA G+C content	1260:1286	The genomic DNA G+C content was 59.0 mol%.
30204584	10	75	theme	DNA	1272:1274	arg1	%					1300:1300	59.0 mol%	1292:1300	59.0 mol%	1292:1300	The genomic DNA G+C content was 59.0 mol%.
30204584	1	76	theme	altitude	59:66	arg1	lake					68:71	a high altitude lake	52:71	a high altitude lake	52:71	nov., isolated from a high altitude lake.
30204584	0	77	theme	lacuslunae	17:26	arg1	sp					28:29	Psychromicrobium lacuslunae sp	0:29	Psychromicrobium lacuslunae sp.	0:30	Psychromicrobium lacuslunae sp.
30204584	5	78	theme	sequence	496:503	arg1	analysis					505:512	The 16S rRNA gene sequence analysis	478:512	The 16S rRNA gene sequence analysis	478:512	The 16S rRNA gene sequence analysis revealed the highest relatedness of strain IHBB 11108T with Psychromicrobium silvestre DSM 102047T (97.5 %), Arthrobacter russicus DSM 14555T (97.4 %) and Renibacterium salmoninarum ATCC 33209T (97.4 %).
30204584	12	79	theme	name	1676:1679	arg1	sp					1709:1710	the name Psychromicrobium lacuslunae sp	1672:1710	the name Psychromicrobium lacuslunae sp	1672:1710	On the basis of the results of the phenotypic, chemotaxonomic and phylogenetic analyses, IHBB 11108T is considered to represent a novel species of the genus Psychromicrobium for which the name Psychromicrobium lacuslunae sp.
30204584	2	80	theme	subsurface	170:179	arg1	water					181:185	the subsurface water	166:185	the subsurface water of Chandra Tal Lake	166:205	The novel strain IHBB 11108T was a psychrotolerant and alkaliphilic bacterium isolated from the subsurface water of Chandra Tal Lake in the Lahaul-Spiti valley located in the Indian trans-Himalayas.
30204584	7	81	theme	unidentified	985:996	arg1	glycolipid					998:1007	one unidentified glycolipid	981:1007	one unidentified glycolipid	981:1007	The polar lipids detected were diphosphatidylglycerol, dimannosylglyceride, phosphatidylinositol, phosphatidylglycerol, monogalactosyldiacylglycerol, one unidentified glycolipid and four unidentified lipids.
30204584	14	82	theme	2780T=JCM	1780:1788	arg1	11108T					1755:1760	IHBB 11108T	1750:1760	IHBB 11108T (=MTCC 12460T=MCC 2780T=JCM 31143T=KACC 19070T)	1750:1808	The type strain is IHBB 11108T (=MTCC 12460T=MCC 2780T=JCM 31143T=KACC 19070T).
30204584	14	82	theme	2780T=JCM	1780:1788	arg1	19070T					1802:1807	=MTCC 12460T=MCC 2780T=JCM 31143T=KACC 19070T	1763:1807	=MTCC 12460T=MCC 2780T=JCM 31143T=KACC 19070T	1763:1807	The type strain is IHBB 11108T (=MTCC 12460T=MCC 2780T=JCM 31143T=KACC 19070T).
30204584	2	83	attach	isolated	152:159	arg2	bacterium					142:150	a psychrotolerant and alkaliphilic bacterium	107:150	a psychrotolerant and alkaliphilic bacterium isolated from the subsurface water of Chandra Tal Lake	107:205	The novel strain IHBB 11108T was a psychrotolerant and alkaliphilic bacterium isolated from the subsurface water of Chandra Tal Lake in the Lahaul-Spiti valley located in the Indian trans-Himalayas.
30204584	2	83	attach	isolated	152:159	arg2	11108T					96:101	The novel strain IHBB 11108T	74:101	The novel strain IHBB 11108T	74:101	The novel strain IHBB 11108T was a psychrotolerant and alkaliphilic bacterium isolated from the subsurface water of Chandra Tal Lake in the Lahaul-Spiti valley located in the Indian trans-Himalayas.
30204584	2	83	attach	isolated	152:159	arg1	water					181:185	the subsurface water	166:185	the subsurface water of Chandra Tal Lake	166:205	The novel strain IHBB 11108T was a psychrotolerant and alkaliphilic bacterium isolated from the subsurface water of Chandra Tal Lake in the Lahaul-Spiti valley located in the Indian trans-Himalayas.
30204584	4	84	theme	1 	472:473	arg1	%					474:474	optimum 1 %	464:474	optimum 1 %	464:474	The strain grew at 5-37 °C (optimum 28 °C), pH 5.0-12.0 (optimum pH 7.0) and with up to 8 % NaCl (optimum 1 %).
30204584	4	84	theme	1 	472:473	arg1	NaCl					458:461	up to 8 % NaCl	448:461	up to 8 % NaCl (optimum 1 %)	448:475	The strain grew at 5-37 °C (optimum 28 °C), pH 5.0-12.0 (optimum pH 7.0) and with up to 8 % NaCl (optimum 1 %).
30204584	11	85	theme	A.	1418:1419	arg1	14555T					1434:1439	A. russicus DSM 14555T	1418:1439	A. russicus DSM 14555T	1418:1439	The DNA-DNA relatedness of strain IHBB 11108T was 46.7±2.2, 43.1±2.5 and 19.1±2.4 % with P. silvestre DSM 102047T, A. russicus DSM 14555T and R. salmoninarum ATCC 33209T, respectively.
30204584	4	86	dep	NaCl	458:461	arg1	%					456:456	%	456:456	%	456:456	The strain grew at 5-37 °C (optimum 28 °C), pH 5.0-12.0 (optimum pH 7.0) and with up to 8 % NaCl (optimum 1 %).
30204584	12	87	theme	Psychromicrobium	1645:1660	arg1	species					1624:1630	a novel species	1616:1630	a novel species of the genus Psychromicrobium for which the name Psychromicrobium lacuslunae sp	1616:1710	On the basis of the results of the phenotypic, chemotaxonomic and phylogenetic analyses, IHBB 11108T is considered to represent a novel species of the genus Psychromicrobium for which the name Psychromicrobium lacuslunae sp.
30204584	14	88	theme	=MTCC	1763:1767	arg1	11108T					1755:1760	IHBB 11108T	1750:1760	IHBB 11108T (=MTCC 12460T=MCC 2780T=JCM 31143T=KACC 19070T)	1750:1808	The type strain is IHBB 11108T (=MTCC 12460T=MCC 2780T=JCM 31143T=KACC 19070T).
30204584	14	88	theme	=MTCC	1763:1767	arg1	19070T					1802:1807	=MTCC 12460T=MCC 2780T=JCM 31143T=KACC 19070T	1763:1807	=MTCC 12460T=MCC 2780T=JCM 31143T=KACC 19070T	1763:1807	The type strain is IHBB 11108T (=MTCC 12460T=MCC 2780T=JCM 31143T=KACC 19070T).
30204584	12	89	theme	novel	1618:1622	arg1	species					1624:1630	a novel species	1616:1630	a novel species of the genus Psychromicrobium for which the name Psychromicrobium lacuslunae sp	1616:1710	On the basis of the results of the phenotypic, chemotaxonomic and phylogenetic analyses, IHBB 11108T is considered to represent a novel species of the genus Psychromicrobium for which the name Psychromicrobium lacuslunae sp.
30204584	4	90	dep	8 	454:455	arg1	to					451:452	to	451:452	to	451:452	The strain grew at 5-37 °C (optimum 28 °C), pH 5.0-12.0 (optimum pH 7.0) and with up to 8 % NaCl (optimum 1 %).
30204584	8	91	theme	group	1144:1148	arg1	substitution					1113:1124	substitution	1113:1124	substitution of the α-carboxyl group of d-Glu by alanine amide	1113:1174	The cell-wall peptidoglycan structure type was A3α l-Lys-l-Thr-l-Ala with substitution of the α-carboxyl group of d-Glu by alanine amide.
30204584	8	92	theme	A3α	1086:1088	arg1	l-Lys-l-Thr-l-Ala					1090:1106	A3α l-Lys-l-Thr-l-Ala	1086:1106	A3α l-Lys-l-Thr-l-Ala	1086:1106	The cell-wall peptidoglycan structure type was A3α l-Lys-l-Thr-l-Ala with substitution of the α-carboxyl group of d-Glu by alanine amide.
30204584	11	93	theme	DSM	1430:1432	arg1	14555T					1434:1439	A. russicus DSM 14555T	1418:1439	A. russicus DSM 14555T	1418:1439	The DNA-DNA relatedness of strain IHBB 11108T was 46.7±2.2, 43.1±2.5 and 19.1±2.4 % with P. silvestre DSM 102047T, A. russicus DSM 14555T and R. salmoninarum ATCC 33209T, respectively.
30204584	7	94	theme	unidentified	1018:1029	arg1	lipids					1031:1036	four unidentified lipids	1013:1036	four unidentified lipids	1013:1036	The polar lipids detected were diphosphatidylglycerol, dimannosylglyceride, phosphatidylinositol, phosphatidylglycerol, monogalactosyldiacylglycerol, one unidentified glycolipid and four unidentified lipids.
30204584	9	95	theme	fatty	1247:1251	arg1	Anteiso-C15 					1177:1188	Anteiso-C15 	1177:1188	Anteiso-C15 	1177:1188	Anteiso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0 were the predominant fatty acids.
30204584	9	95	theme	fatty	1247:1251	arg1	acids					1253:1257	the predominant fatty acids	1231:1257	the predominant fatty acids	1231:1257	Anteiso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0 were the predominant fatty acids.
31009737	11	0	theme	UV-induced	1465:1474	arg1	skin					1487:1490	UV-induced photoaging skin	1465:1490	UV-induced photoaging skin	1465:1490	RPP and LSGYGP exerted an additive effect on the amelioration of the biochemical indices of UV-induced photoaging skin.
31009737	7	1	theme	antioxidant	834:844	arg1	activities					853:862	antioxidant enzyme activities	834:862	antioxidant enzyme activities	834:862	The biochemical indices of mice skin, including composition (collagen and hyaluronic acid [HA] contents), oxidant stress (antioxidant enzyme activities and glutathione and malondialdehyde contents), MMPs (MMP-1, MMP-3, and MMP-9 levels), inflammatory cytokines (interleukin (IL)-1α, tumor nuclear factor-α, and IL-6 levels) and the phosphorylation of the mitogen-activated protein kinase pathway, were determined.
31009737	7	2	theme	biochemical	716:726	arg1	composition					760:770	composition	760:770	composition (collagen and hyaluronic acid [HA] contents)	760:815	The biochemical indices of mice skin, including composition (collagen and hyaluronic acid [HA] contents), oxidant stress (antioxidant enzyme activities and glutathione and malondialdehyde contents), MMPs (MMP-1, MMP-3, and MMP-9 levels), inflammatory cytokines (interleukin (IL)-1α, tumor nuclear factor-α, and IL-6 levels) and the phosphorylation of the mitogen-activated protein kinase pathway, were determined.
31009737	7	2	theme	biochemical	716:726	arg1	indices					728:734	The biochemical indices	712:734	The biochemical indices	712:734	The biochemical indices of mice skin, including composition (collagen and hyaluronic acid [HA] contents), oxidant stress (antioxidant enzyme activities and glutathione and malondialdehyde contents), MMPs (MMP-1, MMP-3, and MMP-9 levels), inflammatory cytokines (interleukin (IL)-1α, tumor nuclear factor-α, and IL-6 levels) and the phosphorylation of the mitogen-activated protein kinase pathway, were determined.
31009737	7	2	theme	biochemical	716:726	arg1	phosphorylation					1044:1058	the phosphorylation	1040:1058	the phosphorylation of the mitogen-activated protein kinase pathway	1040:1106	The biochemical indices of mice skin, including composition (collagen and hyaluronic acid [HA] contents), oxidant stress (antioxidant enzyme activities and glutathione and malondialdehyde contents), MMPs (MMP-1, MMP-3, and MMP-9 levels), inflammatory cytokines (interleukin (IL)-1α, tumor nuclear factor-α, and IL-6 levels) and the phosphorylation of the mitogen-activated protein kinase pathway, were determined.
31009737	7	2	theme	biochemical	716:726	arg1	cytokines					963:971	inflammatory cytokines	950:971	inflammatory cytokines (interleukin (IL)-1α, tumor nuclear factor-α, and IL-6 levels)	950:1034	The biochemical indices of mice skin, including composition (collagen and hyaluronic acid [HA] contents), oxidant stress (antioxidant enzyme activities and glutathione and malondialdehyde contents), MMPs (MMP-1, MMP-3, and MMP-9 levels), inflammatory cytokines (interleukin (IL)-1α, tumor nuclear factor-α, and IL-6 levels) and the phosphorylation of the mitogen-activated protein kinase pathway, were determined.
31009737	7	2	theme	biochemical	716:726	arg1	MMPs					911:914	MMPs	911:914	MMPs (MMP-1, MMP-3, and MMP-9 levels)	911:947	The biochemical indices of mice skin, including composition (collagen and hyaluronic acid [HA] contents), oxidant stress (antioxidant enzyme activities and glutathione and malondialdehyde contents), MMPs (MMP-1, MMP-3, and MMP-9 levels), inflammatory cytokines (interleukin (IL)-1α, tumor nuclear factor-α, and IL-6 levels) and the phosphorylation of the mitogen-activated protein kinase pathway, were determined.
31009737	7	2	theme	biochemical	716:726	arg1	stress					826:831	oxidant stress	818:831	oxidant stress (antioxidant enzyme activities and glutathione and malondialdehyde contents)	818:908	The biochemical indices of mice skin, including composition (collagen and hyaluronic acid [HA] contents), oxidant stress (antioxidant enzyme activities and glutathione and malondialdehyde contents), MMPs (MMP-1, MMP-3, and MMP-9 levels), inflammatory cytokines (interleukin (IL)-1α, tumor nuclear factor-α, and IL-6 levels) and the phosphorylation of the mitogen-activated protein kinase pathway, were determined.
31009737	11	3	theme	indices	1454:1460	arg1	amelioration					1422:1433	the amelioration	1418:1433	the amelioration of the biochemical indices of UV-induced photoaging skin	1418:1490	RPP and LSGYGP exerted an additive effect on the amelioration of the biochemical indices of UV-induced photoaging skin.
31009737	8	4	theme	RPP	1164:1166	arg1	effect					1154:1159	a protective effect	1141:1159	a protective effect of RPP and/or LSGYGP on photoaging skin	1141:1199	Results showed a protective effect of RPP and/or LSGYGP on photoaging skin.
31009737	5	5	theme	mice	624:627	arg1	photoaging					634:643	ultraviolet (UV)-induced hairless mice skin photoaging	590:643	ultraviolet (UV)-induced hairless mice skin photoaging	590:643	We evaluated the effects of RPP and/or LSGYGP on ultraviolet (UV)-induced hairless mice skin photoaging.
31009737	10	6	theme	inflammatory	1343:1354	arg1	levels					1365:1370	inflammatory cytokine levels	1343:1370	inflammatory cytokine levels	1343:1370	RPP showed improved effects on the regulation of the oxidant stress and inflammatory cytokine levels.
31009737	2	7	theme	radical	288:294	arg1	activity					307:314	good radical scavenging activity	283:314	good radical scavenging activity	283:314	Leu-Ser-Gly-Tyr-Gly-Pro (LSGYGP) possesses good radical scavenging activity and matrix metalloproteinase (MMPs) inhibitory ability.
31009737	7	8	theme	hyaluronic	786:795	arg1	composition					760:770	composition	760:770	composition (collagen and hyaluronic acid [HA] contents)	760:815	The biochemical indices of mice skin, including composition (collagen and hyaluronic acid [HA] contents), oxidant stress (antioxidant enzyme activities and glutathione and malondialdehyde contents), MMPs (MMP-1, MMP-3, and MMP-9 levels), inflammatory cytokines (interleukin (IL)-1α, tumor nuclear factor-α, and IL-6 levels) and the phosphorylation of the mitogen-activated protein kinase pathway, were determined.
31009737	7	8	theme	hyaluronic	786:795	arg1	[HA					802:804	hyaluronic acid [HA	786:804	hyaluronic acid [HA	786:804	The biochemical indices of mice skin, including composition (collagen and hyaluronic acid [HA] contents), oxidant stress (antioxidant enzyme activities and glutathione and malondialdehyde contents), MMPs (MMP-1, MMP-3, and MMP-9 levels), inflammatory cytokines (interleukin (IL)-1α, tumor nuclear factor-α, and IL-6 levels) and the phosphorylation of the mitogen-activated protein kinase pathway, were determined.
31009737	8	9	theme	LSGYGP	1175:1180	arg1	effect					1154:1159	a protective effect	1141:1159	a protective effect of RPP and/or LSGYGP on photoaging skin	1141:1199	Results showed a protective effect of RPP and/or LSGYGP on photoaging skin.
31009737	10	10	theme	stress	1332:1337	arg1	regulation					1306:1315	the regulation	1302:1315	the regulation of the oxidant stress and inflammatory cytokine levels	1302:1370	RPP showed improved effects on the regulation of the oxidant stress and inflammatory cytokine levels.
31009737	8	11	theme	photoaging	1185:1194	arg1	skin					1196:1199	photoaging skin	1185:1199	photoaging skin	1185:1199	Results showed a protective effect of RPP and/or LSGYGP on photoaging skin.
31009737	4	12	from	effects	478:484	arg1	photoaging					515:524	in vivo photoaging	507:524	in vivo photoaging	507:524	Few data on the effects of RPP and LSGYGP on in vivo photoaging are available.
31009737	2	13	theme	matrix	320:325	arg1	metalloproteinase					327:343	matrix metalloproteinase	320:343	matrix metalloproteinase (MMPs) inhibitory ability	320:369	Leu-Ser-Gly-Tyr-Gly-Pro (LSGYGP) possesses good radical scavenging activity and matrix metalloproteinase (MMPs) inhibitory ability.
31009737	2	13	theme	matrix	320:325	arg1	MMPs					346:349	MMPs	346:349	MMPs	346:349	Leu-Ser-Gly-Tyr-Gly-Pro (LSGYGP) possesses good radical scavenging activity and matrix metalloproteinase (MMPs) inhibitory ability.
31009737	1	14	theme	antioxidant	194:204	arg1	activities					228:237	high antioxidant and anti-inflammatory activities	189:237	high antioxidant and anti-inflammatory activities	189:237	Rambutan peel phenolics (RPP) have high antioxidant and anti-inflammatory activities.
31009737	0	15	from	Leu-Ser-Gly-Tyr-Gly-Pro	61:83	arg1	mice					97:100	hairless mice	88:100	hairless mice skin photoaging induced by ultraviolet irradiation	88:151	Effects of rambutan (Nephelium lappaceum) peel phenolics and Leu-Ser-Gly-Tyr-Gly-Pro on hairless mice skin photoaging induced by ultraviolet irradiation.
31009737	0	16	theme	ultraviolet	129:139	arg1	irradiation					141:151	ultraviolet irradiation	129:151	ultraviolet irradiation	129:151	Effects of rambutan (Nephelium lappaceum) peel phenolics and Leu-Ser-Gly-Tyr-Gly-Pro on hairless mice skin photoaging induced by ultraviolet irradiation.
31009737	1	17	theme	anti-inflammatory	210:226	arg1	activities					228:237	high antioxidant and anti-inflammatory activities	189:237	high antioxidant and anti-inflammatory activities	189:237	Rambutan peel phenolics (RPP) have high antioxidant and anti-inflammatory activities.
31009737	0	18	from	phenolics	47:55	arg1	mice					97:100	hairless mice	88:100	hairless mice skin photoaging induced by ultraviolet irradiation	88:151	Effects of rambutan (Nephelium lappaceum) peel phenolics and Leu-Ser-Gly-Tyr-Gly-Pro on hairless mice skin photoaging induced by ultraviolet irradiation.
31009737	5	19	theme	RPP	569:571	arg1	effects					558:564	the effects	554:564	the effects of RPP and/or LSGYGP on ultraviolet (UV)-induced hairless mice skin photoaging	554:643	We evaluated the effects of RPP and/or LSGYGP on ultraviolet (UV)-induced hairless mice skin photoaging.
31009737	4	20	theme	Few	462:464	arg1	data					466:469	Few data	462:469	Few data on the effects of RPP and LSGYGP on in vivo photoaging	462:524	Few data on the effects of RPP and LSGYGP on in vivo photoaging are available.
31009737	7	21	theme	kinase	1093:1098	arg1	pathway					1100:1106	the mitogen-activated protein kinase pathway	1063:1106	the mitogen-activated protein kinase pathway	1063:1106	The biochemical indices of mice skin, including composition (collagen and hyaluronic acid [HA] contents), oxidant stress (antioxidant enzyme activities and glutathione and malondialdehyde contents), MMPs (MMP-1, MMP-3, and MMP-9 levels), inflammatory cytokines (interleukin (IL)-1α, tumor nuclear factor-α, and IL-6 levels) and the phosphorylation of the mitogen-activated protein kinase pathway, were determined.
31009737	1	22	theme	Rambutan	154:161	arg1	RPP					179:181	RPP	179:181	RPP	179:181	Rambutan peel phenolics (RPP) have high antioxidant and anti-inflammatory activities.
31009737	1	22	theme	Rambutan	154:161	arg1	phenolics					168:176	Rambutan peel phenolics	154:176	Rambutan peel phenolics (RPP)	154:182	Rambutan peel phenolics (RPP) have high antioxidant and anti-inflammatory activities.
31009737	4	23	theme	in	507:508	arg1	photoaging					515:524	in vivo photoaging	507:524	in vivo photoaging	507:524	Few data on the effects of RPP and LSGYGP on in vivo photoaging are available.
31009737	11	24	theme	biochemical	1442:1452	arg1	indices					1454:1460	the biochemical indices	1438:1460	the biochemical indices of UV-induced photoaging skin	1438:1490	RPP and LSGYGP exerted an additive effect on the amelioration of the biochemical indices of UV-induced photoaging skin.
31009737	9	25	theme	considerable	1216:1227	arg1	effects					1229:1235	considerable effects	1216:1235	considerable effects on skin collagen and HA contents	1216:1268	LSGYGP showed considerable effects on skin collagen and HA contents.
31009737	10	26	theme	levels	1365:1370	arg1	regulation					1306:1315	the regulation	1302:1315	the regulation of the oxidant stress and inflammatory cytokine levels	1302:1370	RPP showed improved effects on the regulation of the oxidant stress and inflammatory cytokine levels.
31009737	0	27	theme	hairless	88:95	arg1	mice					97:100	hairless mice	88:100	hairless mice skin photoaging induced by ultraviolet irradiation	88:151	Effects of rambutan (Nephelium lappaceum) peel phenolics and Leu-Ser-Gly-Tyr-Gly-Pro on hairless mice skin photoaging induced by ultraviolet irradiation.
31009737	7	28	theme	MMP-1	917:921	arg1	MMP-3					924:928	MMP-1, MMP-3, and MMP-9 levels	917:946	MMP-3	924:928	The biochemical indices of mice skin, including composition (collagen and hyaluronic acid [HA] contents), oxidant stress (antioxidant enzyme activities and glutathione and malondialdehyde contents), MMPs (MMP-1, MMP-3, and MMP-9 levels), inflammatory cytokines (interleukin (IL)-1α, tumor nuclear factor-α, and IL-6 levels) and the phosphorylation of the mitogen-activated protein kinase pathway, were determined.
31009737	12	29	theme	endogenous	1586:1595	arg1	collagen					1597:1604	endogenous collagen	1586:1604	endogenous collagen	1586:1604	The histological changes showed that RPP and LSGYGP recovered the changes in skin tissue and endogenous collagen.
31009737	4	30	theme	RPP	489:491	arg1	effects					478:484	the effects	474:484	the effects of RPP and LSGYGP on in vivo photoaging	474:524	Few data on the effects of RPP and LSGYGP on in vivo photoaging are available.
31009737	6	31	theme	RPP	696:698	arg1	effect					686:691	the additive effect	673:691	the additive effect of RPP and LSGYGP	673:709	In particular, we analyzed the additive effect of RPP and LSGYGP.
31009737	0	32	theme	skin	102:105	arg1	photoaging					107:116	skin photoaging	102:116	hairless mice skin photoaging induced by ultraviolet irradiation	88:151	Effects of rambutan (Nephelium lappaceum) peel phenolics and Leu-Ser-Gly-Tyr-Gly-Pro on hairless mice skin photoaging induced by ultraviolet irradiation.
31009737	4	33	theme	LSGYGP	497:502	arg1	effects					478:484	the effects	474:484	the effects of RPP and LSGYGP on in vivo photoaging	474:524	Few data on the effects of RPP and LSGYGP on in vivo photoaging are available.
31009737	9	34	theme	HA	1258:1259	arg1	contents					1261:1268	HA contents	1258:1268	HA contents	1258:1268	LSGYGP showed considerable effects on skin collagen and HA contents.
31009737	0	35	theme	rambutan	11:18	arg1	Effects					0:6	Effects	0:6	Effects of rambutan (Nephelium lappaceum)	0:40	Effects of rambutan (Nephelium lappaceum) peel phenolics and Leu-Ser-Gly-Tyr-Gly-Pro on hairless mice skin photoaging induced by ultraviolet irradiation.
31009737	7	36	theme	mitogen-activated	1067:1083	arg1	pathway					1100:1106	the mitogen-activated protein kinase pathway	1063:1106	the mitogen-activated protein kinase pathway	1063:1106	The biochemical indices of mice skin, including composition (collagen and hyaluronic acid [HA] contents), oxidant stress (antioxidant enzyme activities and glutathione and malondialdehyde contents), MMPs (MMP-1, MMP-3, and MMP-9 levels), inflammatory cytokines (interleukin (IL)-1α, tumor nuclear factor-α, and IL-6 levels) and the phosphorylation of the mitogen-activated protein kinase pathway, were determined.
31009737	7	37	theme	IL-6	1023:1026	arg1	levels					1028:1033	IL-6 levels	1023:1033	IL-6 levels	1023:1033	The biochemical indices of mice skin, including composition (collagen and hyaluronic acid [HA] contents), oxidant stress (antioxidant enzyme activities and glutathione and malondialdehyde contents), MMPs (MMP-1, MMP-3, and MMP-9 levels), inflammatory cytokines (interleukin (IL)-1α, tumor nuclear factor-α, and IL-6 levels) and the phosphorylation of the mitogen-activated protein kinase pathway, were determined.
31009737	2	38	theme	inhibitory	352:361	arg1	ability					363:369	matrix metalloproteinase (MMPs) inhibitory ability	320:369	matrix metalloproteinase (MMPs) inhibitory ability	320:369	Leu-Ser-Gly-Tyr-Gly-Pro (LSGYGP) possesses good radical scavenging activity and matrix metalloproteinase (MMPs) inhibitory ability.
31009737	7	39	theme	mice	739:742	arg1	skin					744:747	mice skin	739:747	mice skin	739:747	The biochemical indices of mice skin, including composition (collagen and hyaluronic acid [HA] contents), oxidant stress (antioxidant enzyme activities and glutathione and malondialdehyde contents), MMPs (MMP-1, MMP-3, and MMP-9 levels), inflammatory cytokines (interleukin (IL)-1α, tumor nuclear factor-α, and IL-6 levels) and the phosphorylation of the mitogen-activated protein kinase pathway, were determined.
31009737	3	40	used	used	437:440	arg2	LSGYGP					423:428	LSGYGP	423:428	LSGYGP	423:428	These underlying mechanisms indicated that RPP and LSGYGP may be used for antiphotoaging.
31009737	3	40	used	used	437:440	arg2	RPP					415:417	RPP	415:417	RPP	415:417	These underlying mechanisms indicated that RPP and LSGYGP may be used for antiphotoaging.
31009737	0	41	dep	rambutan	11:18	arg1	lappaceum					31:39	Nephelium lappaceum	21:39	Nephelium lappaceum	21:39	Effects of rambutan (Nephelium lappaceum) peel phenolics and Leu-Ser-Gly-Tyr-Gly-Pro on hairless mice skin photoaging induced by ultraviolet irradiation.
31009737	7	42	dep	collagen	773:780	arg1	contents					807:814	contents	807:814	contents	807:814	The biochemical indices of mice skin, including composition (collagen and hyaluronic acid [HA] contents), oxidant stress (antioxidant enzyme activities and glutathione and malondialdehyde contents), MMPs (MMP-1, MMP-3, and MMP-9 levels), inflammatory cytokines (interleukin (IL)-1α, tumor nuclear factor-α, and IL-6 levels) and the phosphorylation of the mitogen-activated protein kinase pathway, were determined.
31009737	6	43	theme	LSGYGP	704:709	arg1	effect					686:691	the additive effect	673:691	the additive effect of RPP and LSGYGP	673:709	In particular, we analyzed the additive effect of RPP and LSGYGP.
31009737	11	44	theme	photoaging	1476:1485	arg1	skin					1487:1490	UV-induced photoaging skin	1465:1490	UV-induced photoaging skin	1465:1490	RPP and LSGYGP exerted an additive effect on the amelioration of the biochemical indices of UV-induced photoaging skin.
31009737	7	45	theme	enzyme	846:851	arg1	activities					853:862	antioxidant enzyme activities	834:862	antioxidant enzyme activities	834:862	The biochemical indices of mice skin, including composition (collagen and hyaluronic acid [HA] contents), oxidant stress (antioxidant enzyme activities and glutathione and malondialdehyde contents), MMPs (MMP-1, MMP-3, and MMP-9 levels), inflammatory cytokines (interleukin (IL)-1α, tumor nuclear factor-α, and IL-6 levels) and the phosphorylation of the mitogen-activated protein kinase pathway, were determined.
31009737	2	46	contain	possesses	273:281	arg1	Leu-Ser-Gly-Tyr-Gly-Pro					240:262	Leu-Ser-Gly-Tyr-Gly-Pro	240:262	Leu-Ser-Gly-Tyr-Gly-Pro (LSGYGP)	240:271	Leu-Ser-Gly-Tyr-Gly-Pro (LSGYGP) possesses good radical scavenging activity and matrix metalloproteinase (MMPs) inhibitory ability.
31009737	2	46	contain	possesses	273:281	arg2	activity					307:314	good radical scavenging activity	283:314	good radical scavenging activity	283:314	Leu-Ser-Gly-Tyr-Gly-Pro (LSGYGP) possesses good radical scavenging activity and matrix metalloproteinase (MMPs) inhibitory ability.
31009737	2	46	contain	possesses	273:281	arg1	LSGYGP					265:270	LSGYGP	265:270	LSGYGP	265:270	Leu-Ser-Gly-Tyr-Gly-Pro (LSGYGP) possesses good radical scavenging activity and matrix metalloproteinase (MMPs) inhibitory ability.
31009737	2	46	contain	possesses	273:281	arg2	ability					363:369	matrix metalloproteinase (MMPs) inhibitory ability	320:369	matrix metalloproteinase (MMPs) inhibitory ability	320:369	Leu-Ser-Gly-Tyr-Gly-Pro (LSGYGP) possesses good radical scavenging activity and matrix metalloproteinase (MMPs) inhibitory ability.
31009737	8	47	from	effect	1154:1159	arg1	skin					1196:1199	photoaging skin	1185:1199	photoaging skin	1185:1199	Results showed a protective effect of RPP and/or LSGYGP on photoaging skin.
31009737	5	48	theme	hairless	615:622	arg1	photoaging					634:643	ultraviolet (UV)-induced hairless mice skin photoaging	590:643	ultraviolet (UV)-induced hairless mice skin photoaging	590:643	We evaluated the effects of RPP and/or LSGYGP on ultraviolet (UV)-induced hairless mice skin photoaging.
31009737	2	49	theme	scavenging	296:305	arg1	activity					307:314	good radical scavenging activity	283:314	good radical scavenging activity	283:314	Leu-Ser-Gly-Tyr-Gly-Pro (LSGYGP) possesses good radical scavenging activity and matrix metalloproteinase (MMPs) inhibitory ability.
31009737	5	50	theme	skin	629:632	arg1	photoaging					634:643	ultraviolet (UV)-induced hairless mice skin photoaging	590:643	ultraviolet (UV)-induced hairless mice skin photoaging	590:643	We evaluated the effects of RPP and/or LSGYGP on ultraviolet (UV)-induced hairless mice skin photoaging.
31009737	7	51	theme	oxidant	818:824	arg1	stress					826:831	oxidant stress	818:831	oxidant stress (antioxidant enzyme activities and glutathione and malondialdehyde contents)	818:908	The biochemical indices of mice skin, including composition (collagen and hyaluronic acid [HA] contents), oxidant stress (antioxidant enzyme activities and glutathione and malondialdehyde contents), MMPs (MMP-1, MMP-3, and MMP-9 levels), inflammatory cytokines (interleukin (IL)-1α, tumor nuclear factor-α, and IL-6 levels) and the phosphorylation of the mitogen-activated protein kinase pathway, were determined.
31009737	11	52	theme	skin	1487:1490	arg1	indices					1454:1460	the biochemical indices	1438:1460	the biochemical indices of UV-induced photoaging skin	1438:1490	RPP and LSGYGP exerted an additive effect on the amelioration of the biochemical indices of UV-induced photoaging skin.
31009737	7	53	theme	tumor	995:999	arg1	factor-α					1009:1016	tumor nuclear factor-α	995:1016	tumor nuclear factor-α	995:1016	The biochemical indices of mice skin, including composition (collagen and hyaluronic acid [HA] contents), oxidant stress (antioxidant enzyme activities and glutathione and malondialdehyde contents), MMPs (MMP-1, MMP-3, and MMP-9 levels), inflammatory cytokines (interleukin (IL)-1α, tumor nuclear factor-α, and IL-6 levels) and the phosphorylation of the mitogen-activated protein kinase pathway, were determined.
31009737	7	53	theme	tumor	995:999	arg1	cytokines					963:971	inflammatory cytokines	950:971	inflammatory cytokines (interleukin (IL)-1α, tumor nuclear factor-α, and IL-6 levels)	950:1034	The biochemical indices of mice skin, including composition (collagen and hyaluronic acid [HA] contents), oxidant stress (antioxidant enzyme activities and glutathione and malondialdehyde contents), MMPs (MMP-1, MMP-3, and MMP-9 levels), inflammatory cytokines (interleukin (IL)-1α, tumor nuclear factor-α, and IL-6 levels) and the phosphorylation of the mitogen-activated protein kinase pathway, were determined.
31009737	10	54	theme	oxidant	1324:1330	arg1	stress					1332:1337	the oxidant stress	1320:1337	the oxidant stress	1320:1337	RPP showed improved effects on the regulation of the oxidant stress and inflammatory cytokine levels.
31009737	2	55	theme	metalloproteinase	327:343	arg1	ability					363:369	matrix metalloproteinase (MMPs) inhibitory ability	320:369	matrix metalloproteinase (MMPs) inhibitory ability	320:369	Leu-Ser-Gly-Tyr-Gly-Pro (LSGYGP) possesses good radical scavenging activity and matrix metalloproteinase (MMPs) inhibitory ability.
31009737	1	56	theme	high	189:192	arg1	activities					228:237	high antioxidant and anti-inflammatory activities	189:237	high antioxidant and anti-inflammatory activities	189:237	Rambutan peel phenolics (RPP) have high antioxidant and anti-inflammatory activities.
31009737	12	57	from	changes	1559:1565	arg1	tissue					1575:1580	skin tissue	1570:1580	skin tissue	1570:1580	The histological changes showed that RPP and LSGYGP recovered the changes in skin tissue and endogenous collagen.
31009737	12	57	from	changes	1559:1565	arg1	collagen					1597:1604	endogenous collagen	1586:1604	endogenous collagen	1586:1604	The histological changes showed that RPP and LSGYGP recovered the changes in skin tissue and endogenous collagen.
31009737	9	58	from	effects	1229:1235	arg1	collagen					1245:1252	skin collagen	1240:1252	skin collagen	1240:1252	LSGYGP showed considerable effects on skin collagen and HA contents.
31009737	9	58	from	effects	1229:1235	arg1	contents					1261:1268	HA contents	1258:1268	HA contents	1258:1268	LSGYGP showed considerable effects on skin collagen and HA contents.
31009737	4	59	from	data	466:469	arg1	effects					478:484	the effects	474:484	the effects of RPP and LSGYGP on in vivo photoaging	474:524	Few data on the effects of RPP and LSGYGP on in vivo photoaging are available.
31009737	8	60	theme	protective	1143:1152	arg1	effect					1154:1159	a protective effect	1141:1159	a protective effect of RPP and/or LSGYGP on photoaging skin	1141:1199	Results showed a protective effect of RPP and/or LSGYGP on photoaging skin.
31009737	2	61	theme	good	283:286	arg1	activity					307:314	good radical scavenging activity	283:314	good radical scavenging activity	283:314	Leu-Ser-Gly-Tyr-Gly-Pro (LSGYGP) possesses good radical scavenging activity and matrix metalloproteinase (MMPs) inhibitory ability.
31009737	7	62	theme	inflammatory	950:961	arg1	factor-α					1009:1016	tumor nuclear factor-α	995:1016	tumor nuclear factor-α	995:1016	The biochemical indices of mice skin, including composition (collagen and hyaluronic acid [HA] contents), oxidant stress (antioxidant enzyme activities and glutathione and malondialdehyde contents), MMPs (MMP-1, MMP-3, and MMP-9 levels), inflammatory cytokines (interleukin (IL)-1α, tumor nuclear factor-α, and IL-6 levels) and the phosphorylation of the mitogen-activated protein kinase pathway, were determined.
31009737	7	62	theme	inflammatory	950:961	arg1	cytokines					963:971	inflammatory cytokines	950:971	inflammatory cytokines (interleukin (IL)-1α, tumor nuclear factor-α, and IL-6 levels)	950:1034	The biochemical indices of mice skin, including composition (collagen and hyaluronic acid [HA] contents), oxidant stress (antioxidant enzyme activities and glutathione and malondialdehyde contents), MMPs (MMP-1, MMP-3, and MMP-9 levels), inflammatory cytokines (interleukin (IL)-1α, tumor nuclear factor-α, and IL-6 levels) and the phosphorylation of the mitogen-activated protein kinase pathway, were determined.
31009737	7	62	theme	inflammatory	950:961	arg1	interleukin					974:984	interleukin (IL)-1α	974:992	interleukin (IL)-1α	974:992	The biochemical indices of mice skin, including composition (collagen and hyaluronic acid [HA] contents), oxidant stress (antioxidant enzyme activities and glutathione and malondialdehyde contents), MMPs (MMP-1, MMP-3, and MMP-9 levels), inflammatory cytokines (interleukin (IL)-1α, tumor nuclear factor-α, and IL-6 levels) and the phosphorylation of the mitogen-activated protein kinase pathway, were determined.
31009737	11	63	theme	additive	1399:1406	arg1	effect					1408:1413	an additive effect	1396:1413	an additive effect	1396:1413	RPP and LSGYGP exerted an additive effect on the amelioration of the biochemical indices of UV-induced photoaging skin.
31009737	12	64	theme	histological	1497:1508	arg1	changes					1510:1516	The histological changes	1493:1516	The histological changes	1493:1516	The histological changes showed that RPP and LSGYGP recovered the changes in skin tissue and endogenous collagen.
31009737	7	65	theme	MMP-9	935:939	arg1	levels					941:946	MMP-1, MMP-3, and MMP-9 levels	917:946	levels	941:946	The biochemical indices of mice skin, including composition (collagen and hyaluronic acid [HA] contents), oxidant stress (antioxidant enzyme activities and glutathione and malondialdehyde contents), MMPs (MMP-1, MMP-3, and MMP-9 levels), inflammatory cytokines (interleukin (IL)-1α, tumor nuclear factor-α, and IL-6 levels) and the phosphorylation of the mitogen-activated protein kinase pathway, were determined.
31009737	1	66	theme	peel	163:166	arg1	RPP					179:181	RPP	179:181	RPP	179:181	Rambutan peel phenolics (RPP) have high antioxidant and anti-inflammatory activities.
31009737	1	66	theme	peel	163:166	arg1	phenolics					168:176	Rambutan peel phenolics	154:176	Rambutan peel phenolics (RPP)	154:182	Rambutan peel phenolics (RPP) have high antioxidant and anti-inflammatory activities.
31009737	7	67	theme	nuclear	1001:1007	arg1	factor-α					1009:1016	tumor nuclear factor-α	995:1016	tumor nuclear factor-α	995:1016	The biochemical indices of mice skin, including composition (collagen and hyaluronic acid [HA] contents), oxidant stress (antioxidant enzyme activities and glutathione and malondialdehyde contents), MMPs (MMP-1, MMP-3, and MMP-9 levels), inflammatory cytokines (interleukin (IL)-1α, tumor nuclear factor-α, and IL-6 levels) and the phosphorylation of the mitogen-activated protein kinase pathway, were determined.
31009737	7	67	theme	nuclear	1001:1007	arg1	cytokines					963:971	inflammatory cytokines	950:971	inflammatory cytokines (interleukin (IL)-1α, tumor nuclear factor-α, and IL-6 levels)	950:1034	The biochemical indices of mice skin, including composition (collagen and hyaluronic acid [HA] contents), oxidant stress (antioxidant enzyme activities and glutathione and malondialdehyde contents), MMPs (MMP-1, MMP-3, and MMP-9 levels), inflammatory cytokines (interleukin (IL)-1α, tumor nuclear factor-α, and IL-6 levels) and the phosphorylation of the mitogen-activated protein kinase pathway, were determined.
31009737	7	68	dep	MMPs	911:914	arg1	levels					941:946	MMP-1, MMP-3, and MMP-9 levels	917:946	levels	941:946	The biochemical indices of mice skin, including composition (collagen and hyaluronic acid [HA] contents), oxidant stress (antioxidant enzyme activities and glutathione and malondialdehyde contents), MMPs (MMP-1, MMP-3, and MMP-9 levels), inflammatory cytokines (interleukin (IL)-1α, tumor nuclear factor-α, and IL-6 levels) and the phosphorylation of the mitogen-activated protein kinase pathway, were determined.
31009737	7	68	dep	MMPs	911:914	arg1	MMP-3					924:928	MMP-1, MMP-3, and MMP-9 levels	917:946	MMP-3	924:928	The biochemical indices of mice skin, including composition (collagen and hyaluronic acid [HA] contents), oxidant stress (antioxidant enzyme activities and glutathione and malondialdehyde contents), MMPs (MMP-1, MMP-3, and MMP-9 levels), inflammatory cytokines (interleukin (IL)-1α, tumor nuclear factor-α, and IL-6 levels) and the phosphorylation of the mitogen-activated protein kinase pathway, were determined.
31009737	10	69	theme	cytokine	1356:1363	arg1	levels					1365:1370	inflammatory cytokine levels	1343:1370	inflammatory cytokine levels	1343:1370	RPP showed improved effects on the regulation of the oxidant stress and inflammatory cytokine levels.
31009737	9	70	theme	skin	1240:1243	arg1	collagen					1245:1252	skin collagen	1240:1252	skin collagen	1240:1252	LSGYGP showed considerable effects on skin collagen and HA contents.
31009737	5	71	theme	-induced	606:613	arg1	photoaging					634:643	ultraviolet (UV)-induced hairless mice skin photoaging	590:643	ultraviolet (UV)-induced hairless mice skin photoaging	590:643	We evaluated the effects of RPP and/or LSGYGP on ultraviolet (UV)-induced hairless mice skin photoaging.
31009737	0	72	dep	mice	97:100	arg1	photoaging					107:116	skin photoaging	102:116	hairless mice skin photoaging induced by ultraviolet irradiation	88:151	Effects of rambutan (Nephelium lappaceum) peel phenolics and Leu-Ser-Gly-Tyr-Gly-Pro on hairless mice skin photoaging induced by ultraviolet irradiation.
31009737	3	73	theme	underlying	378:387	arg1	mechanisms					389:398	These underlying mechanisms	372:398	These underlying mechanisms	372:398	These underlying mechanisms indicated that RPP and LSGYGP may be used for antiphotoaging.
31009737	7	74	dep	cytokines	963:971	arg1	IL					987:988	IL	987:988	IL	987:988	The biochemical indices of mice skin, including composition (collagen and hyaluronic acid [HA] contents), oxidant stress (antioxidant enzyme activities and glutathione and malondialdehyde contents), MMPs (MMP-1, MMP-3, and MMP-9 levels), inflammatory cytokines (interleukin (IL)-1α, tumor nuclear factor-α, and IL-6 levels) and the phosphorylation of the mitogen-activated protein kinase pathway, were determined.
31009737	7	74	dep	cytokines	963:971	arg1	factor-α					1009:1016	tumor nuclear factor-α	995:1016	tumor nuclear factor-α	995:1016	The biochemical indices of mice skin, including composition (collagen and hyaluronic acid [HA] contents), oxidant stress (antioxidant enzyme activities and glutathione and malondialdehyde contents), MMPs (MMP-1, MMP-3, and MMP-9 levels), inflammatory cytokines (interleukin (IL)-1α, tumor nuclear factor-α, and IL-6 levels) and the phosphorylation of the mitogen-activated protein kinase pathway, were determined.
31009737	7	74	dep	cytokines	963:971	arg1	cytokines					963:971	inflammatory cytokines	950:971	inflammatory cytokines (interleukin (IL)-1α, tumor nuclear factor-α, and IL-6 levels)	950:1034	The biochemical indices of mice skin, including composition (collagen and hyaluronic acid [HA] contents), oxidant stress (antioxidant enzyme activities and glutathione and malondialdehyde contents), MMPs (MMP-1, MMP-3, and MMP-9 levels), inflammatory cytokines (interleukin (IL)-1α, tumor nuclear factor-α, and IL-6 levels) and the phosphorylation of the mitogen-activated protein kinase pathway, were determined.
31009737	7	74	dep	cytokines	963:971	arg1	interleukin					974:984	interleukin (IL)-1α	974:992	interleukin (IL)-1α	974:992	The biochemical indices of mice skin, including composition (collagen and hyaluronic acid [HA] contents), oxidant stress (antioxidant enzyme activities and glutathione and malondialdehyde contents), MMPs (MMP-1, MMP-3, and MMP-9 levels), inflammatory cytokines (interleukin (IL)-1α, tumor nuclear factor-α, and IL-6 levels) and the phosphorylation of the mitogen-activated protein kinase pathway, were determined.
31009737	7	74	dep	cytokines	963:971	arg1	levels					1028:1033	IL-6 levels	1023:1033	IL-6 levels	1023:1033	The biochemical indices of mice skin, including composition (collagen and hyaluronic acid [HA] contents), oxidant stress (antioxidant enzyme activities and glutathione and malondialdehyde contents), MMPs (MMP-1, MMP-3, and MMP-9 levels), inflammatory cytokines (interleukin (IL)-1α, tumor nuclear factor-α, and IL-6 levels) and the phosphorylation of the mitogen-activated protein kinase pathway, were determined.
31009737	7	75	theme	pathway	1100:1106	arg1	composition					760:770	composition	760:770	composition (collagen and hyaluronic acid [HA] contents)	760:815	The biochemical indices of mice skin, including composition (collagen and hyaluronic acid [HA] contents), oxidant stress (antioxidant enzyme activities and glutathione and malondialdehyde contents), MMPs (MMP-1, MMP-3, and MMP-9 levels), inflammatory cytokines (interleukin (IL)-1α, tumor nuclear factor-α, and IL-6 levels) and the phosphorylation of the mitogen-activated protein kinase pathway, were determined.
31009737	7	75	theme	pathway	1100:1106	arg1	[HA					802:804	hyaluronic acid [HA	786:804	hyaluronic acid [HA	786:804	The biochemical indices of mice skin, including composition (collagen and hyaluronic acid [HA] contents), oxidant stress (antioxidant enzyme activities and glutathione and malondialdehyde contents), MMPs (MMP-1, MMP-3, and MMP-9 levels), inflammatory cytokines (interleukin (IL)-1α, tumor nuclear factor-α, and IL-6 levels) and the phosphorylation of the mitogen-activated protein kinase pathway, were determined.
31009737	7	75	theme	pathway	1100:1106	arg1	factor-α					1009:1016	tumor nuclear factor-α	995:1016	tumor nuclear factor-α	995:1016	The biochemical indices of mice skin, including composition (collagen and hyaluronic acid [HA] contents), oxidant stress (antioxidant enzyme activities and glutathione and malondialdehyde contents), MMPs (MMP-1, MMP-3, and MMP-9 levels), inflammatory cytokines (interleukin (IL)-1α, tumor nuclear factor-α, and IL-6 levels) and the phosphorylation of the mitogen-activated protein kinase pathway, were determined.
31009737	7	75	theme	pathway	1100:1106	arg1	interleukin					974:984	interleukin (IL)-1α	974:992	interleukin (IL)-1α	974:992	The biochemical indices of mice skin, including composition (collagen and hyaluronic acid [HA] contents), oxidant stress (antioxidant enzyme activities and glutathione and malondialdehyde contents), MMPs (MMP-1, MMP-3, and MMP-9 levels), inflammatory cytokines (interleukin (IL)-1α, tumor nuclear factor-α, and IL-6 levels) and the phosphorylation of the mitogen-activated protein kinase pathway, were determined.
31009737	7	75	theme	pathway	1100:1106	arg1	collagen					773:780	collagen	773:780	collagen	773:780	The biochemical indices of mice skin, including composition (collagen and hyaluronic acid [HA] contents), oxidant stress (antioxidant enzyme activities and glutathione and malondialdehyde contents), MMPs (MMP-1, MMP-3, and MMP-9 levels), inflammatory cytokines (interleukin (IL)-1α, tumor nuclear factor-α, and IL-6 levels) and the phosphorylation of the mitogen-activated protein kinase pathway, were determined.
31009737	7	75	theme	pathway	1100:1106	arg1	phosphorylation					1044:1058	the phosphorylation	1040:1058	the phosphorylation of the mitogen-activated protein kinase pathway	1040:1106	The biochemical indices of mice skin, including composition (collagen and hyaluronic acid [HA] contents), oxidant stress (antioxidant enzyme activities and glutathione and malondialdehyde contents), MMPs (MMP-1, MMP-3, and MMP-9 levels), inflammatory cytokines (interleukin (IL)-1α, tumor nuclear factor-α, and IL-6 levels) and the phosphorylation of the mitogen-activated protein kinase pathway, were determined.
31009737	7	75	theme	pathway	1100:1106	arg1	cytokines					963:971	inflammatory cytokines	950:971	inflammatory cytokines (interleukin (IL)-1α, tumor nuclear factor-α, and IL-6 levels)	950:1034	The biochemical indices of mice skin, including composition (collagen and hyaluronic acid [HA] contents), oxidant stress (antioxidant enzyme activities and glutathione and malondialdehyde contents), MMPs (MMP-1, MMP-3, and MMP-9 levels), inflammatory cytokines (interleukin (IL)-1α, tumor nuclear factor-α, and IL-6 levels) and the phosphorylation of the mitogen-activated protein kinase pathway, were determined.
31009737	7	75	theme	pathway	1100:1106	arg1	MMPs					911:914	MMPs	911:914	MMPs (MMP-1, MMP-3, and MMP-9 levels)	911:947	The biochemical indices of mice skin, including composition (collagen and hyaluronic acid [HA] contents), oxidant stress (antioxidant enzyme activities and glutathione and malondialdehyde contents), MMPs (MMP-1, MMP-3, and MMP-9 levels), inflammatory cytokines (interleukin (IL)-1α, tumor nuclear factor-α, and IL-6 levels) and the phosphorylation of the mitogen-activated protein kinase pathway, were determined.
31009737	7	75	theme	pathway	1100:1106	arg1	stress					826:831	oxidant stress	818:831	oxidant stress (antioxidant enzyme activities and glutathione and malondialdehyde contents)	818:908	The biochemical indices of mice skin, including composition (collagen and hyaluronic acid [HA] contents), oxidant stress (antioxidant enzyme activities and glutathione and malondialdehyde contents), MMPs (MMP-1, MMP-3, and MMP-9 levels), inflammatory cytokines (interleukin (IL)-1α, tumor nuclear factor-α, and IL-6 levels) and the phosphorylation of the mitogen-activated protein kinase pathway, were determined.
31009737	5	76	theme	LSGYGP	580:585	arg1	effects					558:564	the effects	554:564	the effects of RPP and/or LSGYGP on ultraviolet (UV)-induced hairless mice skin photoaging	554:643	We evaluated the effects of RPP and/or LSGYGP on ultraviolet (UV)-induced hairless mice skin photoaging.
31009737	6	77	theme	additive	677:684	arg1	effect					686:691	the additive effect	673:691	the additive effect of RPP and LSGYGP	673:709	In particular, we analyzed the additive effect of RPP and LSGYGP.
31009737	7	78	theme	protein	1085:1091	arg1	pathway					1100:1106	the mitogen-activated protein kinase pathway	1063:1106	the mitogen-activated protein kinase pathway	1063:1106	The biochemical indices of mice skin, including composition (collagen and hyaluronic acid [HA] contents), oxidant stress (antioxidant enzyme activities and glutathione and malondialdehyde contents), MMPs (MMP-1, MMP-3, and MMP-9 levels), inflammatory cytokines (interleukin (IL)-1α, tumor nuclear factor-α, and IL-6 levels) and the phosphorylation of the mitogen-activated protein kinase pathway, were determined.
31009737	7	79	theme	acid	797:800	arg1	composition					760:770	composition	760:770	composition (collagen and hyaluronic acid [HA] contents)	760:815	The biochemical indices of mice skin, including composition (collagen and hyaluronic acid [HA] contents), oxidant stress (antioxidant enzyme activities and glutathione and malondialdehyde contents), MMPs (MMP-1, MMP-3, and MMP-9 levels), inflammatory cytokines (interleukin (IL)-1α, tumor nuclear factor-α, and IL-6 levels) and the phosphorylation of the mitogen-activated protein kinase pathway, were determined.
31009737	7	79	theme	acid	797:800	arg1	[HA					802:804	hyaluronic acid [HA	786:804	hyaluronic acid [HA	786:804	The biochemical indices of mice skin, including composition (collagen and hyaluronic acid [HA] contents), oxidant stress (antioxidant enzyme activities and glutathione and malondialdehyde contents), MMPs (MMP-1, MMP-3, and MMP-9 levels), inflammatory cytokines (interleukin (IL)-1α, tumor nuclear factor-α, and IL-6 levels) and the phosphorylation of the mitogen-activated protein kinase pathway, were determined.
31009737	1	80	contain	have	184:187	arg2	activities					228:237	high antioxidant and anti-inflammatory activities	189:237	high antioxidant and anti-inflammatory activities	189:237	Rambutan peel phenolics (RPP) have high antioxidant and anti-inflammatory activities.
31009737	1	80	contain	have	184:187	arg1	RPP					179:181	RPP	179:181	RPP	179:181	Rambutan peel phenolics (RPP) have high antioxidant and anti-inflammatory activities.
31009737	1	80	contain	have	184:187	arg1	phenolics					168:176	Rambutan peel phenolics	154:176	Rambutan peel phenolics (RPP)	154:182	Rambutan peel phenolics (RPP) have high antioxidant and anti-inflammatory activities.
31009737	12	81	theme	skin	1570:1573	arg1	tissue					1575:1580	skin tissue	1570:1580	skin tissue	1570:1580	The histological changes showed that RPP and LSGYGP recovered the changes in skin tissue and endogenous collagen.
31009737	7	82	dep	stress	826:831	arg1	activities					853:862	antioxidant enzyme activities	834:862	antioxidant enzyme activities	834:862	The biochemical indices of mice skin, including composition (collagen and hyaluronic acid [HA] contents), oxidant stress (antioxidant enzyme activities and glutathione and malondialdehyde contents), MMPs (MMP-1, MMP-3, and MMP-9 levels), inflammatory cytokines (interleukin (IL)-1α, tumor nuclear factor-α, and IL-6 levels) and the phosphorylation of the mitogen-activated protein kinase pathway, were determined.
31009737	7	82	dep	stress	826:831	arg1	glutathione					868:878	glutathione	868:878	glutathione	868:878	The biochemical indices of mice skin, including composition (collagen and hyaluronic acid [HA] contents), oxidant stress (antioxidant enzyme activities and glutathione and malondialdehyde contents), MMPs (MMP-1, MMP-3, and MMP-9 levels), inflammatory cytokines (interleukin (IL)-1α, tumor nuclear factor-α, and IL-6 levels) and the phosphorylation of the mitogen-activated protein kinase pathway, were determined.
31009737	7	83	theme	malondialdehyde	884:898	arg1	contents					900:907	malondialdehyde contents	884:907	malondialdehyde contents	884:907	The biochemical indices of mice skin, including composition (collagen and hyaluronic acid [HA] contents), oxidant stress (antioxidant enzyme activities and glutathione and malondialdehyde contents), MMPs (MMP-1, MMP-3, and MMP-9 levels), inflammatory cytokines (interleukin (IL)-1α, tumor nuclear factor-α, and IL-6 levels) and the phosphorylation of the mitogen-activated protein kinase pathway, were determined.
31009737	4	84	dep	in	507:508	arg1	vivo					510:513	vivo	510:513	vivo	510:513	Few data on the effects of RPP and LSGYGP on in vivo photoaging are available.
31009737	7	85	theme	skin	744:747	arg1	composition					760:770	composition	760:770	composition (collagen and hyaluronic acid [HA] contents)	760:815	The biochemical indices of mice skin, including composition (collagen and hyaluronic acid [HA] contents), oxidant stress (antioxidant enzyme activities and glutathione and malondialdehyde contents), MMPs (MMP-1, MMP-3, and MMP-9 levels), inflammatory cytokines (interleukin (IL)-1α, tumor nuclear factor-α, and IL-6 levels) and the phosphorylation of the mitogen-activated protein kinase pathway, were determined.
31009737	7	85	theme	skin	744:747	arg1	indices					728:734	The biochemical indices	712:734	The biochemical indices	712:734	The biochemical indices of mice skin, including composition (collagen and hyaluronic acid [HA] contents), oxidant stress (antioxidant enzyme activities and glutathione and malondialdehyde contents), MMPs (MMP-1, MMP-3, and MMP-9 levels), inflammatory cytokines (interleukin (IL)-1α, tumor nuclear factor-α, and IL-6 levels) and the phosphorylation of the mitogen-activated protein kinase pathway, were determined.
31009737	7	85	theme	skin	744:747	arg1	phosphorylation					1044:1058	the phosphorylation	1040:1058	the phosphorylation of the mitogen-activated protein kinase pathway	1040:1106	The biochemical indices of mice skin, including composition (collagen and hyaluronic acid [HA] contents), oxidant stress (antioxidant enzyme activities and glutathione and malondialdehyde contents), MMPs (MMP-1, MMP-3, and MMP-9 levels), inflammatory cytokines (interleukin (IL)-1α, tumor nuclear factor-α, and IL-6 levels) and the phosphorylation of the mitogen-activated protein kinase pathway, were determined.
31009737	7	85	theme	skin	744:747	arg1	cytokines					963:971	inflammatory cytokines	950:971	inflammatory cytokines (interleukin (IL)-1α, tumor nuclear factor-α, and IL-6 levels)	950:1034	The biochemical indices of mice skin, including composition (collagen and hyaluronic acid [HA] contents), oxidant stress (antioxidant enzyme activities and glutathione and malondialdehyde contents), MMPs (MMP-1, MMP-3, and MMP-9 levels), inflammatory cytokines (interleukin (IL)-1α, tumor nuclear factor-α, and IL-6 levels) and the phosphorylation of the mitogen-activated protein kinase pathway, were determined.
31009737	7	85	theme	skin	744:747	arg1	MMPs					911:914	MMPs	911:914	MMPs (MMP-1, MMP-3, and MMP-9 levels)	911:947	The biochemical indices of mice skin, including composition (collagen and hyaluronic acid [HA] contents), oxidant stress (antioxidant enzyme activities and glutathione and malondialdehyde contents), MMPs (MMP-1, MMP-3, and MMP-9 levels), inflammatory cytokines (interleukin (IL)-1α, tumor nuclear factor-α, and IL-6 levels) and the phosphorylation of the mitogen-activated protein kinase pathway, were determined.
31009737	7	85	theme	skin	744:747	arg1	stress					826:831	oxidant stress	818:831	oxidant stress (antioxidant enzyme activities and glutathione and malondialdehyde contents)	818:908	The biochemical indices of mice skin, including composition (collagen and hyaluronic acid [HA] contents), oxidant stress (antioxidant enzyme activities and glutathione and malondialdehyde contents), MMPs (MMP-1, MMP-3, and MMP-9 levels), inflammatory cytokines (interleukin (IL)-1α, tumor nuclear factor-α, and IL-6 levels) and the phosphorylation of the mitogen-activated protein kinase pathway, were determined.
31009737	5	86	from	effects	558:564	arg1	photoaging					634:643	ultraviolet (UV)-induced hairless mice skin photoaging	590:643	ultraviolet (UV)-induced hairless mice skin photoaging	590:643	We evaluated the effects of RPP and/or LSGYGP on ultraviolet (UV)-induced hairless mice skin photoaging.
31009737	10	87	theme	improved	1282:1289	arg1	effects					1291:1297	improved effects	1282:1297	improved effects	1282:1297	RPP showed improved effects on the regulation of the oxidant stress and inflammatory cytokine levels.
30990394	5	0	theme	cell-wall	689:697	arg1	type					713:716	The cell-wall peptidoglycan type	685:716	The cell-wall peptidoglycan type	685:716	The cell-wall peptidoglycan type was A3α with an l-Lys-l-Ala2.
30990394	1	1	attach	isolated	83:90	arg2	Micrococcaceae					68:81	the family Micrococcaceae	57:81	the family Micrococcaceae isolated from a natural cave	57:110	nov., a new member of the family Micrococcaceae isolated from a natural cave.
30990394	1	1	attach	isolated	83:90	arg1	cave					107:110	a natural cave	97:110	a natural cave	97:110	nov., a new member of the family Micrococcaceae isolated from a natural cave.
30990394	9	2	theme	anteiso-branched	1092:1107	arg1	components					1126:1135	saturated, unsaturated, anteiso-branched and iso-branched components	1068:1135	saturated, unsaturated, anteiso-branched and iso-branched components	1068:1135	The cellular fatty acids consisted of saturated, unsaturated, anteiso-branched and iso-branched components.
30990394	10	3	theme	genome	1190:1195	arg1	%					1181:1181	68.8 mol%	1173:1181	68.8 mol% (draft genome sequence)	1173:1205	The G+C content of genomic DNA was 68.8 mol% (draft genome sequence).
30990394	10	3	theme	genome	1190:1195	arg1	sequence					1197:1204	draft genome sequence	1184:1204	draft genome sequence	1184:1204	The G+C content of genomic DNA was 68.8 mol% (draft genome sequence).
30990394	2	4	attach	isolated	199:206	arg2	C1-50T					187:192	strain C1-50T	180:192	strain C1-50T	180:192	A Gram-reaction-positive, non-spore-forming, rod-shaped bacterium, strain C1-50T, was isolated from a natural cave in Jeju, Republic of Korea by using the serial dilution plating method.
30990394	2	4	attach	isolated	199:206	arg1	cave					223:226	a natural cave	213:226	a natural cave in Jeju, Republic of Korea	213:253	A Gram-reaction-positive, non-spore-forming, rod-shaped bacterium, strain C1-50T, was isolated from a natural cave in Jeju, Republic of Korea by using the serial dilution plating method.
30990394	2	4	attach	isolated	199:206	arg2	bacterium					169:177	A Gram-reaction-positive, non-spore-forming, rod-shaped bacterium	113:177	A Gram-reaction-positive, non-spore-forming, rod-shaped bacterium	113:177	A Gram-reaction-positive, non-spore-forming, rod-shaped bacterium, strain C1-50T, was isolated from a natural cave in Jeju, Republic of Korea by using the serial dilution plating method.
30990394	5	5	theme	peptidoglycan	699:711	arg1	type					713:716	The cell-wall peptidoglycan type	685:716	The cell-wall peptidoglycan type	685:716	The cell-wall peptidoglycan type was A3α with an l-Lys-l-Ala2.
30990394	11	6	dep	differences	1257:1267	arg1	the					1211:1213	the	1211:1213	the	1211:1213	On the basis of morphological and chemotaxonomic differences and distinct phylogenetic clustering, it was concluded that the organism represents a novel species of a new genus in the family Micrococcaceae, for which the name Specibacter cremeus gen. nov., sp.
30990394	11	6	dep	differences	1257:1267	arg1	basis					1215:1219	basis	1215:1219	basis	1215:1219	On the basis of morphological and chemotaxonomic differences and distinct phylogenetic clustering, it was concluded that the organism represents a novel species of a new genus in the family Micrococcaceae, for which the name Specibacter cremeus gen. nov., sp.
30990394	6	7	theme	Whole-cell	748:757	arg1	sugars					759:764	Whole-cell sugars	748:764	Whole-cell sugars	748:764	Whole-cell sugars consisted largely of glucose and galactose.
30990394	4	8	theme	other	633:637	arg1	members					639:645	other members	633:645	other members of the family	633:659	The 16S rRNA gene sequence similarities between strain C1-50T and other members of the family were lower than 96.0 %.
30990394	10	9	theme	draft	1184:1188	arg1	%					1181:1181	68.8 mol%	1173:1181	68.8 mol% (draft genome sequence)	1173:1205	The G+C content of genomic DNA was 68.8 mol% (draft genome sequence).
30990394	10	9	theme	draft	1184:1188	arg1	sequence					1197:1204	draft genome sequence	1184:1204	draft genome sequence	1184:1204	The G+C content of genomic DNA was 68.8 mol% (draft genome sequence).
30990394	3	10	theme	globiformis	533:543	arg1	%					563:563	96.04 %	557:563	96.04 %	557:563	Results of phylogenetic analysis using 16S rRNA gene sequences showed that strain C1-50T belonged to the family Micrococcaceae but had the highest sequence similarity to Arthrobacter halodurans JSM 078085T (96.18 %) and Arthrobacter globiformis DSM 20124T (96.04 %).
30990394	3	10	theme	globiformis	533:543	arg1	DSM					545:547	Arthrobacter globiformis DSM 20124T	520:554	Arthrobacter globiformis DSM 20124T (96.04 %)	520:564	Results of phylogenetic analysis using 16S rRNA gene sequences showed that strain C1-50T belonged to the family Micrococcaceae but had the highest sequence similarity to Arthrobacter halodurans JSM 078085T (96.18 %) and Arthrobacter globiformis DSM 20124T (96.04 %).
30990394	7	11	theme	predominant	814:824	arg1	menaquinone					826:836	The predominant menaquinone	810:836	The predominant menaquinone	810:836	The predominant menaquinone was MK-9(H2) with smaller components of MK-7(H2) and MK-8(H2).
30990394	7	11	theme	predominant	814:824	arg1	MK-9					842:845	MK-9	842:845	MK-9	842:845	The predominant menaquinone was MK-9(H2) with smaller components of MK-7(H2) and MK-8(H2).
30990394	3	12	theme	phylogenetic	311:322	arg1	analysis					324:331	phylogenetic analysis	311:331	phylogenetic analysis using 16S rRNA gene sequences	311:361	Results of phylogenetic analysis using 16S rRNA gene sequences showed that strain C1-50T belonged to the family Micrococcaceae but had the highest sequence similarity to Arthrobacter halodurans JSM 078085T (96.18 %) and Arthrobacter globiformis DSM 20124T (96.04 %).
30990394	2	13	theme	Gram-reaction-positive	115:136	arg1	bacterium					169:177	A Gram-reaction-positive, non-spore-forming, rod-shaped bacterium	113:177	A Gram-reaction-positive, non-spore-forming, rod-shaped bacterium	113:177	A Gram-reaction-positive, non-spore-forming, rod-shaped bacterium, strain C1-50T, was isolated from a natural cave in Jeju, Republic of Korea by using the serial dilution plating method.
30990394	2	13	theme	Gram-reaction-positive	115:136	arg1	C1-50T					187:192	strain C1-50T	180:192	strain C1-50T	180:192	A Gram-reaction-positive, non-spore-forming, rod-shaped bacterium, strain C1-50T, was isolated from a natural cave in Jeju, Republic of Korea by using the serial dilution plating method.
30990394	13	14	theme	type	1490:1493	arg1	C1-50T					1505:1510	C1-50T	1505:1510	C1-50T (=KCTC 39557T=DSM 100066T)	1505:1537	The type strain is C1-50T (=KCTC 39557T=DSM 100066T).
30990394	13	14	theme	type	1490:1493	arg1	strain					1495:1500	The type strain	1486:1500	The type strain	1486:1500	The type strain is C1-50T (=KCTC 39557T=DSM 100066T).
30990394	7	15	theme	MK-7	878:881	arg1	components					864:873	smaller components	856:873	smaller components of MK-7(H2)	856:885	The predominant menaquinone was MK-9(H2) with smaller components of MK-7(H2) and MK-8(H2).
30990394	11	16	theme	genus	1378:1382	arg1	species					1361:1367	a novel species	1353:1367	a novel species	1353:1367	On the basis of morphological and chemotaxonomic differences and distinct phylogenetic clustering, it was concluded that the organism represents a novel species of a new genus in the family Micrococcaceae, for which the name Specibacter cremeus gen. nov., sp.
30990394	2	17	theme	serial	268:273	arg1	method					292:297	the serial dilution plating method	264:297	the serial dilution plating method	264:297	A Gram-reaction-positive, non-spore-forming, rod-shaped bacterium, strain C1-50T, was isolated from a natural cave in Jeju, Republic of Korea by using the serial dilution plating method.
30990394	3	18	contain	had	431:433	arg1	C1-50T					382:387	strain C1-50T	375:387	strain C1-50T	375:387	Results of phylogenetic analysis using 16S rRNA gene sequences showed that strain C1-50T belonged to the family Micrococcaceae but had the highest sequence similarity to Arthrobacter halodurans JSM 078085T (96.18 %) and Arthrobacter globiformis DSM 20124T (96.04 %).
30990394	3	18	contain	had	431:433	arg2	similarity					456:465	the highest sequence similarity	435:465	the highest sequence similarity	435:465	Results of phylogenetic analysis using 16S rRNA gene sequences showed that strain C1-50T belonged to the family Micrococcaceae but had the highest sequence similarity to Arthrobacter halodurans JSM 078085T (96.18 %) and Arthrobacter globiformis DSM 20124T (96.04 %).
30990394	11	19	theme	morphological	1224:1236	arg1	differences					1257:1267	morphological and chemotaxonomic differences	1224:1267	morphological and chemotaxonomic differences	1224:1267	On the basis of morphological and chemotaxonomic differences and distinct phylogenetic clustering, it was concluded that the organism represents a novel species of a new genus in the family Micrococcaceae, for which the name Specibacter cremeus gen. nov., sp.
30990394	11	20	theme	cremeus	1445:1451	arg1	nov.					1458:1461	Specibacter cremeus gen. nov.	1433:1461	the name Specibacter cremeus gen. nov.	1424:1461	On the basis of morphological and chemotaxonomic differences and distinct phylogenetic clustering, it was concluded that the organism represents a novel species of a new genus in the family Micrococcaceae, for which the name Specibacter cremeus gen. nov., sp.
30990394	1	21	theme	new	43:45	arg1	member					47:52	a new member	41:52	a new member of the family Micrococcaceae isolated from a natural cave	41:110	nov., a new member of the family Micrococcaceae isolated from a natural cave.
30990394	1	21	theme	new	43:45	arg1	nov.					35:38	nov.	35:38	nov.	35:38	nov., a new member of the family Micrococcaceae isolated from a natural cave.
30990394	4	22	theme	strain	615:620	arg1	C1-50T					622:627	strain C1-50T	615:627	strain C1-50T	615:627	The 16S rRNA gene sequence similarities between strain C1-50T and other members of the family were lower than 96.0 %.
30990394	10	23	theme	genomic	1157:1163	arg1	DNA					1165:1167	genomic DNA	1157:1167	genomic DNA	1157:1167	The G+C content of genomic DNA was 68.8 mol% (draft genome sequence).
30990394	3	24	theme	rRNA	343:346	arg1	sequences					353:361	16S rRNA gene sequences	339:361	16S rRNA gene sequences	339:361	Results of phylogenetic analysis using 16S rRNA gene sequences showed that strain C1-50T belonged to the family Micrococcaceae but had the highest sequence similarity to Arthrobacter halodurans JSM 078085T (96.18 %) and Arthrobacter globiformis DSM 20124T (96.04 %).
30990394	4	25	theme	sequence	585:592	arg1	similarities					594:605	The 16S rRNA gene sequence similarities	567:605	The 16S rRNA gene sequence similarities between strain C1-50T and other members of the family	567:659	The 16S rRNA gene sequence similarities between strain C1-50T and other members of the family were lower than 96.0 %.
30990394	4	25	theme	sequence	585:592	arg1	lower					666:670	lower	666:670	lower	666:670	The 16S rRNA gene sequence similarities between strain C1-50T and other members of the family were lower than 96.0 %.
30990394	0	26	theme	cremeus	12:18	arg1	nov.					25:28	Specibacter cremeus gen. nov.	0:28	Specibacter cremeus gen. nov.	0:28	Specibacter cremeus gen. nov., sp.
30990394	11	27	dep	name	1428:1431	arg1	nov.					1458:1461	Specibacter cremeus gen. nov.	1433:1461	the name Specibacter cremeus gen. nov.	1424:1461	On the basis of morphological and chemotaxonomic differences and distinct phylogenetic clustering, it was concluded that the organism represents a novel species of a new genus in the family Micrococcaceae, for which the name Specibacter cremeus gen. nov., sp.
30990394	11	28	from	Micrococcaceae	1398:1411	arg1	species					1361:1367	a novel species	1353:1367	a novel species	1353:1367	On the basis of morphological and chemotaxonomic differences and distinct phylogenetic clustering, it was concluded that the organism represents a novel species of a new genus in the family Micrococcaceae, for which the name Specibacter cremeus gen. nov., sp.
30990394	3	29	theme	strain	375:380	arg1	C1-50T					382:387	strain C1-50T	375:387	strain C1-50T	375:387	Results of phylogenetic analysis using 16S rRNA gene sequences showed that strain C1-50T belonged to the family Micrococcaceae but had the highest sequence similarity to Arthrobacter halodurans JSM 078085T (96.18 %) and Arthrobacter globiformis DSM 20124T (96.04 %).
30990394	0	30	theme	Specibacter	0:10	arg1	nov.					25:28	Specibacter cremeus gen. nov.	0:28	Specibacter cremeus gen. nov.	0:28	Specibacter cremeus gen. nov., sp.
30990394	9	31	theme	iso-branched	1113:1124	arg1	components					1126:1135	saturated, unsaturated, anteiso-branched and iso-branched components	1068:1135	saturated, unsaturated, anteiso-branched and iso-branched components	1068:1135	The cellular fatty acids consisted of saturated, unsaturated, anteiso-branched and iso-branched components.
30990394	4	32	theme	family	654:659	arg1	C1-50T					622:627	strain C1-50T	615:627	strain C1-50T	615:627	The 16S rRNA gene sequence similarities between strain C1-50T and other members of the family were lower than 96.0 %.
30990394	4	32	theme	family	654:659	arg1	members					639:645	other members	633:645	other members of the family	633:659	The 16S rRNA gene sequence similarities between strain C1-50T and other members of the family were lower than 96.0 %.
30990394	8	33	theme	polar	905:909	arg1	lipids					911:916	The polar lipids	901:916	The polar lipids	901:916	The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unidentified glycolipid.
30990394	3	34	theme	Arthrobacter	520:531	arg1	%					563:563	96.04 %	557:563	96.04 %	557:563	Results of phylogenetic analysis using 16S rRNA gene sequences showed that strain C1-50T belonged to the family Micrococcaceae but had the highest sequence similarity to Arthrobacter halodurans JSM 078085T (96.18 %) and Arthrobacter globiformis DSM 20124T (96.04 %).
30990394	3	34	theme	Arthrobacter	520:531	arg1	DSM					545:547	Arthrobacter globiformis DSM 20124T	520:554	Arthrobacter globiformis DSM 20124T (96.04 %)	520:564	Results of phylogenetic analysis using 16S rRNA gene sequences showed that strain C1-50T belonged to the family Micrococcaceae but had the highest sequence similarity to Arthrobacter halodurans JSM 078085T (96.18 %) and Arthrobacter globiformis DSM 20124T (96.04 %).
30990394	9	35	theme	fatty	1043:1047	arg1	acids					1049:1053	The cellular fatty acids	1030:1053	The cellular fatty acids	1030:1053	The cellular fatty acids consisted of saturated, unsaturated, anteiso-branched and iso-branched components.
30990394	3	36	theme	analysis	324:331	arg1	Results					300:306	Results	300:306	Results of phylogenetic analysis using 16S rRNA gene sequences	300:361	Results of phylogenetic analysis using 16S rRNA gene sequences showed that strain C1-50T belonged to the family Micrococcaceae but had the highest sequence similarity to Arthrobacter halodurans JSM 078085T (96.18 %) and Arthrobacter globiformis DSM 20124T (96.04 %).
30990394	7	37	theme	smaller	856:862	arg1	components					864:873	smaller components	856:873	smaller components of MK-7(H2)	856:885	The predominant menaquinone was MK-9(H2) with smaller components of MK-7(H2) and MK-8(H2).
30990394	1	38	theme	family	61:66	arg1	Micrococcaceae					68:81	the family Micrococcaceae	57:81	the family Micrococcaceae isolated from a natural cave	57:110	nov., a new member of the family Micrococcaceae isolated from a natural cave.
30990394	11	39	theme	family	1391:1396	arg1	Micrococcaceae					1398:1411	the family Micrococcaceae	1387:1411	the family Micrococcaceae	1387:1411	On the basis of morphological and chemotaxonomic differences and distinct phylogenetic clustering, it was concluded that the organism represents a novel species of a new genus in the family Micrococcaceae, for which the name Specibacter cremeus gen. nov., sp.
30990394	4	40	theme	16S	571:573	arg1	rRNA					575:578	16S rRNA	571:578	The 16S rRNA gene sequence similarities between strain C1-50T and other members of the family	567:659	The 16S rRNA gene sequence similarities between strain C1-50T and other members of the family were lower than 96.0 %.
30990394	0	41	theme	gen.	20:23	arg1	nov.					25:28	Specibacter cremeus gen. nov.	0:28	Specibacter cremeus gen. nov.	0:28	Specibacter cremeus gen. nov., sp.
30990394	2	42	dep	Gram-reaction-positive	115:136	arg1	rod-shaped					158:167	rod-shaped	158:167	rod-shaped	158:167	A Gram-reaction-positive, non-spore-forming, rod-shaped bacterium, strain C1-50T, was isolated from a natural cave in Jeju, Republic of Korea by using the serial dilution plating method.
30990394	2	42	dep	Gram-reaction-positive	115:136	arg1	non-spore-forming					139:155	non-spore-forming	139:155	non-spore-forming	139:155	A Gram-reaction-positive, non-spore-forming, rod-shaped bacterium, strain C1-50T, was isolated from a natural cave in Jeju, Republic of Korea by using the serial dilution plating method.
30990394	3	43	theme	JSM	494:496	arg1	078085T					498:504	Arthrobacter halodurans JSM 078085T	470:504	Arthrobacter halodurans JSM 078085T (96.18 %)	470:514	Results of phylogenetic analysis using 16S rRNA gene sequences showed that strain C1-50T belonged to the family Micrococcaceae but had the highest sequence similarity to Arthrobacter halodurans JSM 078085T (96.18 %) and Arthrobacter globiformis DSM 20124T (96.04 %).
30990394	3	43	theme	JSM	494:496	arg1	%					513:513	96.18 %	507:513	96.18 %	507:513	Results of phylogenetic analysis using 16S rRNA gene sequences showed that strain C1-50T belonged to the family Micrococcaceae but had the highest sequence similarity to Arthrobacter halodurans JSM 078085T (96.18 %) and Arthrobacter globiformis DSM 20124T (96.04 %).
30990394	1	44	theme	Micrococcaceae	68:81	arg1	member					47:52	a new member	41:52	a new member of the family Micrococcaceae isolated from a natural cave	41:110	nov., a new member of the family Micrococcaceae isolated from a natural cave.
30990394	1	44	theme	Micrococcaceae	68:81	arg1	nov.					35:38	nov.	35:38	nov.	35:38	nov., a new member of the family Micrococcaceae isolated from a natural cave.
30990394	9	45	theme	cellular	1034:1041	arg1	acids					1049:1053	The cellular fatty acids	1030:1053	The cellular fatty acids	1030:1053	The cellular fatty acids consisted of saturated, unsaturated, anteiso-branched and iso-branched components.
30990394	3	46	theme	sequence	447:454	arg1	similarity					456:465	the highest sequence similarity	435:465	the highest sequence similarity	435:465	Results of phylogenetic analysis using 16S rRNA gene sequences showed that strain C1-50T belonged to the family Micrococcaceae but had the highest sequence similarity to Arthrobacter halodurans JSM 078085T (96.18 %) and Arthrobacter globiformis DSM 20124T (96.04 %).
30990394	11	47	from	genus	1378:1382	arg1	Micrococcaceae					1398:1411	the family Micrococcaceae	1387:1411	the family Micrococcaceae	1387:1411	On the basis of morphological and chemotaxonomic differences and distinct phylogenetic clustering, it was concluded that the organism represents a novel species of a new genus in the family Micrococcaceae, for which the name Specibacter cremeus gen. nov., sp.
30990394	2	48	from	cave	223:226	arg1	Republic					237:244	Republic	237:244	Republic	237:244	A Gram-reaction-positive, non-spore-forming, rod-shaped bacterium, strain C1-50T, was isolated from a natural cave in Jeju, Republic of Korea by using the serial dilution plating method.
30990394	4	49	theme	gene	580:583	arg1	similarities					594:605	The 16S rRNA gene sequence similarities	567:605	The 16S rRNA gene sequence similarities between strain C1-50T and other members of the family	567:659	The 16S rRNA gene sequence similarities between strain C1-50T and other members of the family were lower than 96.0 %.
30990394	4	49	theme	gene	580:583	arg1	lower					666:670	lower	666:670	lower	666:670	The 16S rRNA gene sequence similarities between strain C1-50T and other members of the family were lower than 96.0 %.
30990394	8	50	theme	unidentified	1005:1016	arg1	glycolipid					1018:1027	an unidentified glycolipid	1002:1027	an unidentified glycolipid	1002:1027	The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unidentified glycolipid.
30990394	11	51	theme	new	1374:1376	arg1	genus					1378:1382	a new genus	1372:1382	a new genus in the family Micrococcaceae	1372:1411	On the basis of morphological and chemotaxonomic differences and distinct phylogenetic clustering, it was concluded that the organism represents a novel species of a new genus in the family Micrococcaceae, for which the name Specibacter cremeus gen. nov., sp.
30990394	11	52	theme	chemotaxonomic	1242:1255	arg1	differences					1257:1267	morphological and chemotaxonomic differences	1224:1267	morphological and chemotaxonomic differences	1224:1267	On the basis of morphological and chemotaxonomic differences and distinct phylogenetic clustering, it was concluded that the organism represents a novel species of a new genus in the family Micrococcaceae, for which the name Specibacter cremeus gen. nov., sp.
30990394	3	53	theme	family	405:410	arg1	Micrococcaceae					412:425	the family Micrococcaceae	401:425	the family Micrococcaceae	401:425	Results of phylogenetic analysis using 16S rRNA gene sequences showed that strain C1-50T belonged to the family Micrococcaceae but had the highest sequence similarity to Arthrobacter halodurans JSM 078085T (96.18 %) and Arthrobacter globiformis DSM 20124T (96.04 %).
30990394	3	54	theme	gene	348:351	arg1	sequences					353:361	16S rRNA gene sequences	339:361	16S rRNA gene sequences	339:361	Results of phylogenetic analysis using 16S rRNA gene sequences showed that strain C1-50T belonged to the family Micrococcaceae but had the highest sequence similarity to Arthrobacter halodurans JSM 078085T (96.18 %) and Arthrobacter globiformis DSM 20124T (96.04 %).
30990394	13	55	theme	39557T=DSM	1519:1528	arg1	C1-50T					1505:1510	C1-50T	1505:1510	C1-50T (=KCTC 39557T=DSM 100066T)	1505:1537	The type strain is C1-50T (=KCTC 39557T=DSM 100066T).
30990394	13	55	theme	39557T=DSM	1519:1528	arg1	100066T					1530:1536	=KCTC 39557T=DSM 100066T	1513:1536	=KCTC 39557T=DSM 100066T	1513:1536	The type strain is C1-50T (=KCTC 39557T=DSM 100066T).
30990394	0	56	dep	sp	31:32	arg1	nov.					25:28	Specibacter cremeus gen. nov.	0:28	Specibacter cremeus gen. nov.	0:28	Specibacter cremeus gen. nov., sp.
30990394	10	57	theme	DNA	1165:1167	arg1	G+C content					1142:1152	The G+C content	1138:1152	The G+C content of genomic DNA	1138:1167	The G+C content of genomic DNA was 68.8 mol% (draft genome sequence).
30990394	10	57	theme	DNA	1165:1167	arg1	%					1181:1181	68.8 mol%	1173:1181	68.8 mol% (draft genome sequence)	1173:1205	The G+C content of genomic DNA was 68.8 mol% (draft genome sequence).
30990394	3	58	theme	highest	439:445	arg1	similarity					456:465	the highest sequence similarity	435:465	the highest sequence similarity	435:465	Results of phylogenetic analysis using 16S rRNA gene sequences showed that strain C1-50T belonged to the family Micrococcaceae but had the highest sequence similarity to Arthrobacter halodurans JSM 078085T (96.18 %) and Arthrobacter globiformis DSM 20124T (96.04 %).
30990394	11	59	dep	sp	1464:1465	arg1	name					1428:1431	the name	1424:1431	the name Specibacter cremeus gen. nov.	1424:1461	On the basis of morphological and chemotaxonomic differences and distinct phylogenetic clustering, it was concluded that the organism represents a novel species of a new genus in the family Micrococcaceae, for which the name Specibacter cremeus gen. nov., sp.
30990394	11	60	theme	distinct	1273:1280	arg1	clustering					1295:1304	distinct phylogenetic clustering	1273:1304	distinct phylogenetic clustering	1273:1304	On the basis of morphological and chemotaxonomic differences and distinct phylogenetic clustering, it was concluded that the organism represents a novel species of a new genus in the family Micrococcaceae, for which the name Specibacter cremeus gen. nov., sp.
30990394	2	61	theme	plating	284:290	arg1	method					292:297	the serial dilution plating method	264:297	the serial dilution plating method	264:297	A Gram-reaction-positive, non-spore-forming, rod-shaped bacterium, strain C1-50T, was isolated from a natural cave in Jeju, Republic of Korea by using the serial dilution plating method.
30990394	2	62	theme	strain	180:185	arg1	bacterium					169:177	A Gram-reaction-positive, non-spore-forming, rod-shaped bacterium	113:177	A Gram-reaction-positive, non-spore-forming, rod-shaped bacterium	113:177	A Gram-reaction-positive, non-spore-forming, rod-shaped bacterium, strain C1-50T, was isolated from a natural cave in Jeju, Republic of Korea by using the serial dilution plating method.
30990394	2	62	theme	strain	180:185	arg1	C1-50T					187:192	strain C1-50T	180:192	strain C1-50T	180:192	A Gram-reaction-positive, non-spore-forming, rod-shaped bacterium, strain C1-50T, was isolated from a natural cave in Jeju, Republic of Korea by using the serial dilution plating method.
30990394	11	63	theme	gen.	1453:1456	arg1	nov.					1458:1461	Specibacter cremeus gen. nov.	1433:1461	the name Specibacter cremeus gen. nov.	1424:1461	On the basis of morphological and chemotaxonomic differences and distinct phylogenetic clustering, it was concluded that the organism represents a novel species of a new genus in the family Micrococcaceae, for which the name Specibacter cremeus gen. nov., sp.
30990394	9	64	theme	saturated	1068:1076	arg1	components					1126:1135	saturated, unsaturated, anteiso-branched and iso-branched components	1068:1135	saturated, unsaturated, anteiso-branched and iso-branched components	1068:1135	The cellular fatty acids consisted of saturated, unsaturated, anteiso-branched and iso-branched components.
30990394	2	65	theme	dilution	275:282	arg1	method					292:297	the serial dilution plating method	264:297	the serial dilution plating method	264:297	A Gram-reaction-positive, non-spore-forming, rod-shaped bacterium, strain C1-50T, was isolated from a natural cave in Jeju, Republic of Korea by using the serial dilution plating method.
30990394	3	66	dep	Arthrobacter	470:481	arg1	halodurans					483:492	halodurans	483:492	halodurans	483:492	Results of phylogenetic analysis using 16S rRNA gene sequences showed that strain C1-50T belonged to the family Micrococcaceae but had the highest sequence similarity to Arthrobacter halodurans JSM 078085T (96.18 %) and Arthrobacter globiformis DSM 20124T (96.04 %).
30990394	13	67	theme	=KCTC	1513:1517	arg1	C1-50T					1505:1510	C1-50T	1505:1510	C1-50T (=KCTC 39557T=DSM 100066T)	1505:1537	The type strain is C1-50T (=KCTC 39557T=DSM 100066T).
30990394	13	67	theme	=KCTC	1513:1517	arg1	100066T					1530:1536	=KCTC 39557T=DSM 100066T	1513:1536	=KCTC 39557T=DSM 100066T	1513:1536	The type strain is C1-50T (=KCTC 39557T=DSM 100066T).
30990394	11	68	from	species	1361:1367	arg1	Micrococcaceae					1398:1411	the family Micrococcaceae	1387:1411	the family Micrococcaceae	1387:1411	On the basis of morphological and chemotaxonomic differences and distinct phylogenetic clustering, it was concluded that the organism represents a novel species of a new genus in the family Micrococcaceae, for which the name Specibacter cremeus gen. nov., sp.
30990394	1	69	theme	natural	99:105	arg1	cave					107:110	a natural cave	97:110	a natural cave	97:110	nov., a new member of the family Micrococcaceae isolated from a natural cave.
30990394	4	70	theme	rRNA	575:578	arg1	similarities					594:605	The 16S rRNA gene sequence similarities	567:605	The 16S rRNA gene sequence similarities between strain C1-50T and other members of the family	567:659	The 16S rRNA gene sequence similarities between strain C1-50T and other members of the family were lower than 96.0 %.
30990394	4	70	theme	rRNA	575:578	arg1	lower					666:670	lower	666:670	lower	666:670	The 16S rRNA gene sequence similarities between strain C1-50T and other members of the family were lower than 96.0 %.
30990394	2	71	theme	natural	215:221	arg1	cave					223:226	a natural cave	213:226	a natural cave in Jeju, Republic of Korea	213:253	A Gram-reaction-positive, non-spore-forming, rod-shaped bacterium, strain C1-50T, was isolated from a natural cave in Jeju, Republic of Korea by using the serial dilution plating method.
30990394	9	72	theme	unsaturated	1079:1089	arg1	components					1126:1135	saturated, unsaturated, anteiso-branched and iso-branched components	1068:1135	saturated, unsaturated, anteiso-branched and iso-branched components	1068:1135	The cellular fatty acids consisted of saturated, unsaturated, anteiso-branched and iso-branched components.
30990394	3	73	theme	Arthrobacter	470:481	arg1	078085T					498:504	Arthrobacter halodurans JSM 078085T	470:504	Arthrobacter halodurans JSM 078085T (96.18 %)	470:514	Results of phylogenetic analysis using 16S rRNA gene sequences showed that strain C1-50T belonged to the family Micrococcaceae but had the highest sequence similarity to Arthrobacter halodurans JSM 078085T (96.18 %) and Arthrobacter globiformis DSM 20124T (96.04 %).
30990394	3	73	theme	Arthrobacter	470:481	arg1	%					513:513	96.18 %	507:513	96.18 %	507:513	Results of phylogenetic analysis using 16S rRNA gene sequences showed that strain C1-50T belonged to the family Micrococcaceae but had the highest sequence similarity to Arthrobacter halodurans JSM 078085T (96.18 %) and Arthrobacter globiformis DSM 20124T (96.04 %).
30990394	10	74	theme	68.8 mol	1173:1180	arg1	G+C content					1142:1152	The G+C content	1138:1152	The G+C content of genomic DNA	1138:1167	The G+C content of genomic DNA was 68.8 mol% (draft genome sequence).
30990394	10	74	theme	68.8 mol	1173:1180	arg1	%					1181:1181	68.8 mol%	1173:1181	68.8 mol% (draft genome sequence)	1173:1205	The G+C content of genomic DNA was 68.8 mol% (draft genome sequence).
30990394	10	74	theme	68.8 mol	1173:1180	arg1	sequence					1197:1204	draft genome sequence	1184:1204	draft genome sequence	1184:1204	The G+C content of genomic DNA was 68.8 mol% (draft genome sequence).
30990394	11	75	theme	phylogenetic	1282:1293	arg1	clustering					1295:1304	distinct phylogenetic clustering	1273:1304	distinct phylogenetic clustering	1273:1304	On the basis of morphological and chemotaxonomic differences and distinct phylogenetic clustering, it was concluded that the organism represents a novel species of a new genus in the family Micrococcaceae, for which the name Specibacter cremeus gen. nov., sp.
30990394	3	76	theme	16S	339:341	arg1	sequences					353:361	16S rRNA gene sequences	339:361	16S rRNA gene sequences	339:361	Results of phylogenetic analysis using 16S rRNA gene sequences showed that strain C1-50T belonged to the family Micrococcaceae but had the highest sequence similarity to Arthrobacter halodurans JSM 078085T (96.18 %) and Arthrobacter globiformis DSM 20124T (96.04 %).
30990394	11	77	theme	Specibacter	1433:1443	arg1	nov.					1458:1461	Specibacter cremeus gen. nov.	1433:1461	the name Specibacter cremeus gen. nov.	1424:1461	On the basis of morphological and chemotaxonomic differences and distinct phylogenetic clustering, it was concluded that the organism represents a novel species of a new genus in the family Micrococcaceae, for which the name Specibacter cremeus gen. nov., sp.
30990394	11	78	theme	novel	1355:1359	arg1	species					1361:1367	a novel species	1353:1367	a novel species	1353:1367	On the basis of morphological and chemotaxonomic differences and distinct phylogenetic clustering, it was concluded that the organism represents a novel species of a new genus in the family Micrococcaceae, for which the name Specibacter cremeus gen. nov., sp.
30912738	2	0	theme	facultative	105:115	arg1	bacterium					127:135	A rod-shaped, endospore-forming, facultative anaerobic bacterium	72:135	bacterium	127:135	A rod-shaped, endospore-forming, facultative anaerobic bacterium, designated FJAT-45385T, was isolated from soil collected from Devil City in the Xinjiang Autonomous Region in China.
30912738	8	1	theme	nucleotide	870:879	arg1	identity					881:888	The average nucleotide identity	858:888	The average nucleotide identity	858:888	The average nucleotide identity and in silico DNA-DNA hybridization values between strain FJAT-45385T and its closest related species were 67.8 and 35.5 %, respectively, which were much lower than the thresholds commonly used to define species (96 and 70 %, respectively) indicating that it belong to a different taxon.
30912738	8	2	theme	different	1161:1169	arg1	taxon					1171:1175	a different taxon	1159:1175	a different taxon	1159:1175	The average nucleotide identity and in silico DNA-DNA hybridization values between strain FJAT-45385T and its closest related species were 67.8 and 35.5 %, respectively, which were much lower than the thresholds commonly used to define species (96 and 70 %, respectively) indicating that it belong to a different taxon.
30912738	8	3	theme	in	894:895	arg1	hybridization					912:924	in silico DNA-DNA hybridization	894:924	in silico DNA-DNA hybridization	894:924	The average nucleotide identity and in silico DNA-DNA hybridization values between strain FJAT-45385T and its closest related species were 67.8 and 35.5 %, respectively, which were much lower than the thresholds commonly used to define species (96 and 70 %, respectively) indicating that it belong to a different taxon.
30912738	7	4	dep	showed	777:782	arg1	%					854:854	96.0 %	849:854	96.0 %	849:854	Phylogenetic analysis based on 16S rRNA gene sequences affiliated strain FJAT-45385T to the genus Bacillus, and showed the highest sequence similarity to Bacillus wakoensis DSM 2521T (96.0 %).
30912738	12	5	theme	type	1402:1405	arg1	FJAT-45385T					1417:1427	FJAT-45385T	1417:1427	FJAT-45385T (=DSM 104651T=CCTCC AB 2016263T)	1417:1460	The type strain is FJAT-45385T (=DSM 104651T=CCTCC AB 2016263T).
30912738	12	5	theme	type	1402:1405	arg1	strain					1407:1412	The type strain	1398:1412	The type strain	1398:1412	The type strain is FJAT-45385T (=DSM 104651T=CCTCC AB 2016263T).
30912738	5	6	dep	anteiso-C15 	494:505	arg1	 0					507:508	 0	507:508	 0	507:508	The main fatty acids were anteiso-C15 : 0 (37.4 %), iso-C15 : 0 (15.1 %) and C16 : 0 (12.6 %).
30912738	5	6	dep	anteiso-C15 	494:505	arg1	%					559:559	12.6 %	554:559	12.6 %	554:559	The main fatty acids were anteiso-C15 : 0 (37.4 %), iso-C15 : 0 (15.1 %) and C16 : 0 (12.6 %).
30912738	5	6	dep	anteiso-C15 	494:505	arg1	 0					529:530	 0	529:530	 0	529:530	The main fatty acids were anteiso-C15 : 0 (37.4 %), iso-C15 : 0 (15.1 %) and C16 : 0 (12.6 %).
30912738	5	6	dep	anteiso-C15 	494:505	arg1	acids					483:487	The main fatty acids	468:487	The main fatty acids	468:487	The main fatty acids were anteiso-C15 : 0 (37.4 %), iso-C15 : 0 (15.1 %) and C16 : 0 (12.6 %).
30912738	5	6	dep	anteiso-C15 	494:505	arg1	 0					550:551	 0	550:551	 0	550:551	The main fatty acids were anteiso-C15 : 0 (37.4 %), iso-C15 : 0 (15.1 %) and C16 : 0 (12.6 %).
30912738	5	6	dep	anteiso-C15 	494:505	arg1	%					516:516	37.4 %	511:516	37.4 %	511:516	The main fatty acids were anteiso-C15 : 0 (37.4 %), iso-C15 : 0 (15.1 %) and C16 : 0 (12.6 %).
30912738	5	6	dep	anteiso-C15 	494:505	arg1	%					538:538	15.1 %	533:538	15.1 %	533:538	The main fatty acids were anteiso-C15 : 0 (37.4 %), iso-C15 : 0 (15.1 %) and C16 : 0 (12.6 %).
30912738	5	6	dep	anteiso-C15 	494:505	arg1	anteiso-C15 					494:505	anteiso-C15 	494:505	anteiso-C15 	494:505	The main fatty acids were anteiso-C15 : 0 (37.4 %), iso-C15 : 0 (15.1 %) and C16 : 0 (12.6 %).
30912738	4	7	theme	isoprenoid	439:448	arg1	MK-7					462:465	MK-7	462:465	MK-7	462:465	The cell-wall peptidoglycan contained meso-diaminopimelic acid and the isoprenoid quinone was MK-7.
30912738	4	7	theme	isoprenoid	439:448	arg1	quinone					450:456	the isoprenoid quinone	435:456	the isoprenoid quinone	435:456	The cell-wall peptidoglycan contained meso-diaminopimelic acid and the isoprenoid quinone was MK-7.
30912738	8	8	theme	hybridization	912:924	arg1	values					926:931	The average nucleotide identity and in silico DNA-DNA hybridization values	858:931	The average nucleotide identity and in silico DNA-DNA hybridization values between strain FJAT-45385T and its closest related species	858:990	The average nucleotide identity and in silico DNA-DNA hybridization values between strain FJAT-45385T and its closest related species were 67.8 and 35.5 %, respectively, which were much lower than the thresholds commonly used to define species (96 and 70 %, respectively) indicating that it belong to a different taxon.
30912738	10	9	theme	phenotypic	1217:1226	arg1	characters					1228:1237	The phenotypic characters	1213:1237	The phenotypic characters	1213:1237	The phenotypic characters and taxono-genomics study revealed that strain FJAT-45385T represents a novel Bacillus species, for which the name Bacillus urbisdiaboli sp.
30912738	3	10	dep	20-40 °C	278:285	arg1	30 °C					297:301	30 °C	297:301	30 °C	297:301	Growth was observed at 20-40 °C (optimum, 30 °C), pH 7.0-11.0 (pH 9.0) and in 0-10.0 % NaCl (4 %), respectively.
30912738	3	10	dep	20-40 °C	278:285	arg1	optimum					288:294	optimum	288:294	optimum	288:294	Growth was observed at 20-40 °C (optimum, 30 °C), pH 7.0-11.0 (pH 9.0) and in 0-10.0 % NaCl (4 %), respectively.
30912738	8	11	theme	DNA-DNA	904:910	arg1	hybridization					912:924	in silico DNA-DNA hybridization	894:924	in silico DNA-DNA hybridization	894:924	The average nucleotide identity and in silico DNA-DNA hybridization values between strain FJAT-45385T and its closest related species were 67.8 and 35.5 %, respectively, which were much lower than the thresholds commonly used to define species (96 and 70 %, respectively) indicating that it belong to a different taxon.
30912738	7	12	theme	DSM	838:840	arg1	2521T					842:846	Bacillus wakoensis DSM 2521T	819:846	Bacillus wakoensis DSM 2521T	819:846	Phylogenetic analysis based on 16S rRNA gene sequences affiliated strain FJAT-45385T to the genus Bacillus, and showed the highest sequence similarity to Bacillus wakoensis DSM 2521T (96.0 %).
30912738	1	13	attach	isolated	32:39	arg1	soil					46:49	soil	46:49	soil	46:49	nov., isolated from soil sampled in Xinjiang.
30912738	1	13	attach	isolated	32:39	arg2	nov.					26:29	nov.	26:29	nov.	26:29	nov., isolated from soil sampled in Xinjiang.
30912738	8	14	theme	average	862:868	arg1	identity					881:888	The average nucleotide identity	858:888	The average nucleotide identity	858:888	The average nucleotide identity and in silico DNA-DNA hybridization values between strain FJAT-45385T and its closest related species were 67.8 and 35.5 %, respectively, which were much lower than the thresholds commonly used to define species (96 and 70 %, respectively) indicating that it belong to a different taxon.
30912738	3	15	theme	%	340:340	arg1	NaCl					342:345	0-10.0 % NaCl	333:345	0-10.0 % NaCl (4 %)	333:351	Growth was observed at 20-40 °C (optimum, 30 °C), pH 7.0-11.0 (pH 9.0) and in 0-10.0 % NaCl (4 %), respectively.
30912738	3	15	theme	%	340:340	arg1	%					350:350	4 %	348:350	4 %	348:350	Growth was observed at 20-40 °C (optimum, 30 °C), pH 7.0-11.0 (pH 9.0) and in 0-10.0 % NaCl (4 %), respectively.
30912738	10	16	theme	Bacillus	1317:1324	arg1	species					1326:1332	a novel Bacillus species	1309:1332	a novel Bacillus species	1309:1332	The phenotypic characters and taxono-genomics study revealed that strain FJAT-45385T represents a novel Bacillus species, for which the name Bacillus urbisdiaboli sp.
30912738	7	17	theme	Bacillus	819:826	arg1	2521T					842:846	Bacillus wakoensis DSM 2521T	819:846	Bacillus wakoensis DSM 2521T	819:846	Phylogenetic analysis based on 16S rRNA gene sequences affiliated strain FJAT-45385T to the genus Bacillus, and showed the highest sequence similarity to Bacillus wakoensis DSM 2521T (96.0 %).
30912738	7	18	theme	strain	731:736	arg1	FJAT-45385T					738:748	strain FJAT-45385T	731:748	strain FJAT-45385T	731:748	Phylogenetic analysis based on 16S rRNA gene sequences affiliated strain FJAT-45385T to the genus Bacillus, and showed the highest sequence similarity to Bacillus wakoensis DSM 2521T (96.0 %).
30912738	12	19	theme	=DSM	1430:1433	arg1	2016263T					1452:1459	=DSM 104651T=CCTCC AB 2016263T	1430:1459	=DSM 104651T=CCTCC AB 2016263T	1430:1459	The type strain is FJAT-45385T (=DSM 104651T=CCTCC AB 2016263T).
30912738	12	19	theme	=DSM	1430:1433	arg1	FJAT-45385T					1417:1427	FJAT-45385T	1417:1427	FJAT-45385T (=DSM 104651T=CCTCC AB 2016263T)	1417:1460	The type strain is FJAT-45385T (=DSM 104651T=CCTCC AB 2016263T).
30912738	0	20	theme	urbisdiaboli	9:20	arg1	sp					22:23	Bacillus urbisdiaboli sp	0:23	Bacillus urbisdiaboli sp.	0:24	Bacillus urbisdiaboli sp.
30912738	4	21	theme	meso-diaminopimelic	406:424	arg1	acid					426:429	meso-diaminopimelic acid	406:429	meso-diaminopimelic acid	406:429	The cell-wall peptidoglycan contained meso-diaminopimelic acid and the isoprenoid quinone was MK-7.
30912738	8	22	dep	%	1011:1011	arg1	lower					1044:1048	lower	1044:1048	lower	1044:1048	The average nucleotide identity and in silico DNA-DNA hybridization values between strain FJAT-45385T and its closest related species were 67.8 and 35.5 %, respectively, which were much lower than the thresholds commonly used to define species (96 and 70 %, respectively) indicating that it belong to a different taxon.
30912738	7	23	theme	wakoensis	828:836	arg1	2521T					842:846	Bacillus wakoensis DSM 2521T	819:846	Bacillus wakoensis DSM 2521T	819:846	Phylogenetic analysis based on 16S rRNA gene sequences affiliated strain FJAT-45385T to the genus Bacillus, and showed the highest sequence similarity to Bacillus wakoensis DSM 2521T (96.0 %).
30912738	0	24	theme	Bacillus	0:7	arg1	sp					22:23	Bacillus urbisdiaboli sp	0:23	Bacillus urbisdiaboli sp.	0:24	Bacillus urbisdiaboli sp.
30912738	12	25	theme	AB	1449:1450	arg1	2016263T					1452:1459	=DSM 104651T=CCTCC AB 2016263T	1430:1459	=DSM 104651T=CCTCC AB 2016263T	1430:1459	The type strain is FJAT-45385T (=DSM 104651T=CCTCC AB 2016263T).
30912738	12	25	theme	AB	1449:1450	arg1	FJAT-45385T					1417:1427	FJAT-45385T	1417:1427	FJAT-45385T (=DSM 104651T=CCTCC AB 2016263T)	1417:1460	The type strain is FJAT-45385T (=DSM 104651T=CCTCC AB 2016263T).
30912738	8	26	theme	strain	941:946	arg1	FJAT-45385T					948:958	strain FJAT-45385T	941:958	strain FJAT-45385T	941:958	The average nucleotide identity and in silico DNA-DNA hybridization values between strain FJAT-45385T and its closest related species were 67.8 and 35.5 %, respectively, which were much lower than the thresholds commonly used to define species (96 and 70 %, respectively) indicating that it belong to a different taxon.
30912738	10	27	theme	taxono-genomics	1243:1257	arg1	study					1259:1263	taxono-genomics study	1243:1263	taxono-genomics study	1243:1263	The phenotypic characters and taxono-genomics study revealed that strain FJAT-45385T represents a novel Bacillus species, for which the name Bacillus urbisdiaboli sp.
30912738	2	28	from	Region	238:243	arg1	China					248:252	China	248:252	China	248:252	A rod-shaped, endospore-forming, facultative anaerobic bacterium, designated FJAT-45385T, was isolated from soil collected from Devil City in the Xinjiang Autonomous Region in China.
30912738	7	29	theme	16S	696:698	arg1	sequences					710:718	16S rRNA gene sequences	696:718	16S rRNA gene sequences affiliated strain FJAT-45385T to the genus Bacillus	696:770	Phylogenetic analysis based on 16S rRNA gene sequences affiliated strain FJAT-45385T to the genus Bacillus, and showed the highest sequence similarity to Bacillus wakoensis DSM 2521T (96.0 %).
30912738	8	30	theme	identity	881:888	arg1	values					926:931	The average nucleotide identity and in silico DNA-DNA hybridization values	858:931	The average nucleotide identity and in silico DNA-DNA hybridization values between strain FJAT-45385T and its closest related species	858:990	The average nucleotide identity and in silico DNA-DNA hybridization values between strain FJAT-45385T and its closest related species were 67.8 and 35.5 %, respectively, which were much lower than the thresholds commonly used to define species (96 and 70 %, respectively) indicating that it belong to a different taxon.
30912738	2	31	attach	isolated	166:173	arg2	bacterium					127:135	A rod-shaped, endospore-forming, facultative anaerobic bacterium	72:135	bacterium	127:135	A rod-shaped, endospore-forming, facultative anaerobic bacterium, designated FJAT-45385T, was isolated from soil collected from Devil City in the Xinjiang Autonomous Region in China.
30912738	2	31	attach	isolated	166:173	arg1	soil					180:183	soil	180:183	soil collected from Devil City in the Xinjiang Autonomous Region in China	180:252	A rod-shaped, endospore-forming, facultative anaerobic bacterium, designated FJAT-45385T, was isolated from soil collected from Devil City in the Xinjiang Autonomous Region in China.
30912738	8	32	dep	species	1094:1100	arg1	%					1113:1113	96 and 70 %	1103:1113	96 and 70 %	1103:1113	The average nucleotide identity and in silico DNA-DNA hybridization values between strain FJAT-45385T and its closest related species were 67.8 and 35.5 %, respectively, which were much lower than the thresholds commonly used to define species (96 and 70 %, respectively) indicating that it belong to a different taxon.
30912738	7	33	theme	rRNA	700:703	arg1	sequences					710:718	16S rRNA gene sequences	696:718	16S rRNA gene sequences affiliated strain FJAT-45385T to the genus Bacillus	696:770	Phylogenetic analysis based on 16S rRNA gene sequences affiliated strain FJAT-45385T to the genus Bacillus, and showed the highest sequence similarity to Bacillus wakoensis DSM 2521T (96.0 %).
30912738	9	34	theme	DNA	1182:1184	arg1	G+C content					1186:1196	The DNA G+C content	1178:1196	The DNA G+C content	1178:1196	The DNA G+C content was 38.1 mol%.
30912738	9	34	theme	DNA	1182:1184	arg1	%					1210:1210	38.1 mol%	1202:1210	38.1 mol%	1202:1210	The DNA G+C content was 38.1 mol%.
30912738	8	35	theme	related	976:982	arg1	species					984:990	its closest related species	964:990	its closest related species	964:990	The average nucleotide identity and in silico DNA-DNA hybridization values between strain FJAT-45385T and its closest related species were 67.8 and 35.5 %, respectively, which were much lower than the thresholds commonly used to define species (96 and 70 %, respectively) indicating that it belong to a different taxon.
30912738	2	36	theme	Xinjiang	218:225	arg1	Region					238:243	the Xinjiang Autonomous Region	214:243	the Xinjiang Autonomous Region in China	214:252	A rod-shaped, endospore-forming, facultative anaerobic bacterium, designated FJAT-45385T, was isolated from soil collected from Devil City in the Xinjiang Autonomous Region in China.
30912738	7	37	theme	gene	705:708	arg1	sequences					710:718	16S rRNA gene sequences	696:718	16S rRNA gene sequences affiliated strain FJAT-45385T to the genus Bacillus	696:770	Phylogenetic analysis based on 16S rRNA gene sequences affiliated strain FJAT-45385T to the genus Bacillus, and showed the highest sequence similarity to Bacillus wakoensis DSM 2521T (96.0 %).
30912738	3	38	located	observed	266:273	arg1	20-40 °C					278:285	20-40 °C	278:285	20-40 °C (optimum, 30 °C)	278:302	Growth was observed at 20-40 °C (optimum, 30 °C), pH 7.0-11.0 (pH 9.0) and in 0-10.0 % NaCl (4 %), respectively.
30912738	3	38	located	observed	266:273	arg1	pH					305:306	pH 7.0-11.0	305:315	pH 7.0-11.0 (pH 9.0)	305:324	Growth was observed at 20-40 °C (optimum, 30 °C), pH 7.0-11.0 (pH 9.0) and in 0-10.0 % NaCl (4 %), respectively.
30912738	3	38	located	observed	266:273	arg2	Growth					255:260	Growth	255:260	Growth	255:260	Growth was observed at 20-40 °C (optimum, 30 °C), pH 7.0-11.0 (pH 9.0) and in 0-10.0 % NaCl (4 %), respectively.
30912738	8	39	theme	closest	968:974	arg1	species					984:990	its closest related species	964:990	its closest related species	964:990	The average nucleotide identity and in silico DNA-DNA hybridization values between strain FJAT-45385T and its closest related species were 67.8 and 35.5 %, respectively, which were much lower than the thresholds commonly used to define species (96 and 70 %, respectively) indicating that it belong to a different taxon.
30912738	4	40	theme	cell-wall	372:380	arg1	peptidoglycan					382:394	The cell-wall peptidoglycan	368:394	The cell-wall peptidoglycan	368:394	The cell-wall peptidoglycan contained meso-diaminopimelic acid and the isoprenoid quinone was MK-7.
30912738	5	41	theme	main	472:475	arg1	 0					529:530	 0	529:530	 0	529:530	The main fatty acids were anteiso-C15 : 0 (37.4 %), iso-C15 : 0 (15.1 %) and C16 : 0 (12.6 %).
30912738	5	41	theme	main	472:475	arg1	 0					507:508	 0	507:508	 0	507:508	The main fatty acids were anteiso-C15 : 0 (37.4 %), iso-C15 : 0 (15.1 %) and C16 : 0 (12.6 %).
30912738	5	41	theme	main	472:475	arg1	anteiso-C15 					494:505	anteiso-C15 	494:505	anteiso-C15 	494:505	The main fatty acids were anteiso-C15 : 0 (37.4 %), iso-C15 : 0 (15.1 %) and C16 : 0 (12.6 %).
30912738	5	41	theme	main	472:475	arg1	acids					483:487	The main fatty acids	468:487	The main fatty acids	468:487	The main fatty acids were anteiso-C15 : 0 (37.4 %), iso-C15 : 0 (15.1 %) and C16 : 0 (12.6 %).
30912738	5	41	theme	main	472:475	arg1	 0					550:551	 0	550:551	 0	550:551	The main fatty acids were anteiso-C15 : 0 (37.4 %), iso-C15 : 0 (15.1 %) and C16 : 0 (12.6 %).
30912738	8	42	dep	in	894:895	arg1	silico					897:902	silico	897:902	silico	897:902	The average nucleotide identity and in silico DNA-DNA hybridization values between strain FJAT-45385T and its closest related species were 67.8 and 35.5 %, respectively, which were much lower than the thresholds commonly used to define species (96 and 70 %, respectively) indicating that it belong to a different taxon.
30912738	3	43	theme	0-10.0 	333:339	arg1	%					340:340	%	340:340	%	340:340	Growth was observed at 20-40 °C (optimum, 30 °C), pH 7.0-11.0 (pH 9.0) and in 0-10.0 % NaCl (4 %), respectively.
30912738	2	44	theme	rod-shaped	74:83	arg1	bacterium					127:135	A rod-shaped, endospore-forming, facultative anaerobic bacterium	72:135	bacterium	127:135	A rod-shaped, endospore-forming, facultative anaerobic bacterium, designated FJAT-45385T, was isolated from soil collected from Devil City in the Xinjiang Autonomous Region in China.
30912738	12	45	theme	104651T=CCTCC	1435:1447	arg1	2016263T					1452:1459	=DSM 104651T=CCTCC AB 2016263T	1430:1459	=DSM 104651T=CCTCC AB 2016263T	1430:1459	The type strain is FJAT-45385T (=DSM 104651T=CCTCC AB 2016263T).
30912738	12	45	theme	104651T=CCTCC	1435:1447	arg1	FJAT-45385T					1417:1427	FJAT-45385T	1417:1427	FJAT-45385T (=DSM 104651T=CCTCC AB 2016263T)	1417:1460	The type strain is FJAT-45385T (=DSM 104651T=CCTCC AB 2016263T).
30912738	5	46	theme	fatty	477:481	arg1	 0					529:530	 0	529:530	 0	529:530	The main fatty acids were anteiso-C15 : 0 (37.4 %), iso-C15 : 0 (15.1 %) and C16 : 0 (12.6 %).
30912738	5	46	theme	fatty	477:481	arg1	 0					507:508	 0	507:508	 0	507:508	The main fatty acids were anteiso-C15 : 0 (37.4 %), iso-C15 : 0 (15.1 %) and C16 : 0 (12.6 %).
30912738	5	46	theme	fatty	477:481	arg1	anteiso-C15 					494:505	anteiso-C15 	494:505	anteiso-C15 	494:505	The main fatty acids were anteiso-C15 : 0 (37.4 %), iso-C15 : 0 (15.1 %) and C16 : 0 (12.6 %).
30912738	5	46	theme	fatty	477:481	arg1	acids					483:487	The main fatty acids	468:487	The main fatty acids	468:487	The main fatty acids were anteiso-C15 : 0 (37.4 %), iso-C15 : 0 (15.1 %) and C16 : 0 (12.6 %).
30912738	5	46	theme	fatty	477:481	arg1	 0					550:551	 0	550:551	 0	550:551	The main fatty acids were anteiso-C15 : 0 (37.4 %), iso-C15 : 0 (15.1 %) and C16 : 0 (12.6 %).
30912738	2	47	dep	rod-shaped	74:83	arg1	endospore-forming					86:102	endospore-forming	86:102	endospore-forming	86:102	A rod-shaped, endospore-forming, facultative anaerobic bacterium, designated FJAT-45385T, was isolated from soil collected from Devil City in the Xinjiang Autonomous Region in China.
30912738	9	48	theme	38.1 mol	1202:1209	arg1	G+C content					1186:1196	The DNA G+C content	1178:1196	The DNA G+C content	1178:1196	The DNA G+C content was 38.1 mol%.
30912738	9	48	theme	38.1 mol	1202:1209	arg1	%					1210:1210	38.1 mol%	1202:1210	38.1 mol%	1202:1210	The DNA G+C content was 38.1 mol%.
30912738	10	49	theme	urbisdiaboli	1363:1374	arg1	sp					1376:1377	the name Bacillus urbisdiaboli sp	1345:1377	the name Bacillus urbisdiaboli sp	1345:1377	The phenotypic characters and taxono-genomics study revealed that strain FJAT-45385T represents a novel Bacillus species, for which the name Bacillus urbisdiaboli sp.
30912738	10	50	theme	strain	1279:1284	arg1	FJAT-45385T					1286:1296	strain FJAT-45385T	1279:1296	strain FJAT-45385T	1279:1296	The phenotypic characters and taxono-genomics study revealed that strain FJAT-45385T represents a novel Bacillus species, for which the name Bacillus urbisdiaboli sp.
30912738	7	51	theme	highest	788:794	arg1	similarity					805:814	the highest sequence similarity	784:814	the highest sequence similarity	784:814	Phylogenetic analysis based on 16S rRNA gene sequences affiliated strain FJAT-45385T to the genus Bacillus, and showed the highest sequence similarity to Bacillus wakoensis DSM 2521T (96.0 %).
30912738	10	52	theme	novel	1311:1315	arg1	species					1326:1332	a novel Bacillus species	1309:1332	a novel Bacillus species	1309:1332	The phenotypic characters and taxono-genomics study revealed that strain FJAT-45385T represents a novel Bacillus species, for which the name Bacillus urbisdiaboli sp.
30912738	10	53	theme	Bacillus	1354:1361	arg1	sp					1376:1377	the name Bacillus urbisdiaboli sp	1345:1377	the name Bacillus urbisdiaboli sp	1345:1377	The phenotypic characters and taxono-genomics study revealed that strain FJAT-45385T represents a novel Bacillus species, for which the name Bacillus urbisdiaboli sp.
30912738	7	54	dep	genus	757:761	arg1	Bacillus					763:770	the genus Bacillus	753:770	the genus Bacillus	753:770	Phylogenetic analysis based on 16S rRNA gene sequences affiliated strain FJAT-45385T to the genus Bacillus, and showed the highest sequence similarity to Bacillus wakoensis DSM 2521T (96.0 %).
30912738	7	55	theme	sequence	796:803	arg1	similarity					805:814	the highest sequence similarity	784:814	the highest sequence similarity	784:814	Phylogenetic analysis based on 16S rRNA gene sequences affiliated strain FJAT-45385T to the genus Bacillus, and showed the highest sequence similarity to Bacillus wakoensis DSM 2521T (96.0 %).
30912738	10	56	theme	name	1349:1352	arg1	sp					1376:1377	the name Bacillus urbisdiaboli sp	1345:1377	the name Bacillus urbisdiaboli sp	1345:1377	The phenotypic characters and taxono-genomics study revealed that strain FJAT-45385T represents a novel Bacillus species, for which the name Bacillus urbisdiaboli sp.
30912738	4	57	contain	contained	396:404	arg1	peptidoglycan					382:394	The cell-wall peptidoglycan	368:394	The cell-wall peptidoglycan	368:394	The cell-wall peptidoglycan contained meso-diaminopimelic acid and the isoprenoid quinone was MK-7.
30912738	4	57	contain	contained	396:404	arg2	acid					426:429	meso-diaminopimelic acid	406:429	meso-diaminopimelic acid	406:429	The cell-wall peptidoglycan contained meso-diaminopimelic acid and the isoprenoid quinone was MK-7.
30912738	6	58	theme	polar	572:576	arg1	diphosphatidylglycerol					590:611	diphosphatidylglycerol	590:611	diphosphatidylglycerol	590:611	The main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine.
30912738	6	58	theme	polar	572:576	arg1	lipids					578:583	The main polar lipids	563:583	The main polar lipids	563:583	The main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine.
30912738	2	59	theme	anaerobic	117:125	arg1	bacterium					127:135	A rod-shaped, endospore-forming, facultative anaerobic bacterium	72:135	bacterium	127:135	A rod-shaped, endospore-forming, facultative anaerobic bacterium, designated FJAT-45385T, was isolated from soil collected from Devil City in the Xinjiang Autonomous Region in China.
30912738	2	60	theme	Autonomous	227:236	arg1	Region					238:243	the Xinjiang Autonomous Region	214:243	the Xinjiang Autonomous Region in China	214:252	A rod-shaped, endospore-forming, facultative anaerobic bacterium, designated FJAT-45385T, was isolated from soil collected from Devil City in the Xinjiang Autonomous Region in China.
30912738	6	61	theme	main	567:570	arg1	diphosphatidylglycerol					590:611	diphosphatidylglycerol	590:611	diphosphatidylglycerol	590:611	The main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine.
30912738	6	61	theme	main	567:570	arg1	lipids					578:583	The main polar lipids	563:583	The main polar lipids	563:583	The main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine.
30912738	7	62	theme	Phylogenetic	665:676	arg1	analysis					678:685	Phylogenetic analysis	665:685	Phylogenetic analysis	665:685	Phylogenetic analysis based on 16S rRNA gene sequences affiliated strain FJAT-45385T to the genus Bacillus, and showed the highest sequence similarity to Bacillus wakoensis DSM 2521T (96.0 %).
30460700	4	0	dep	fat	899:901	arg1	[SHB					916:919	shea butter [SHB	904:919	saturated fatty-acid-rich fat; shea butter [SHB	873:919	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	2	1	theme	saturation	352:361	arg1	degrees					341:347	different degrees	331:347	different degrees of saturation	331:361	However, despite being the largest dietary fatty-acid class, effects of 18-carbon fatty acids with different degrees of saturation on inflammatory, metabolic, and thermogenic markers have not been well studied.
30460700	6	2	theme	adipose	1261:1267	arg1	tissue					1269:1274	brown adipose tissue	1255:1274	the highest brown adipose tissue (BAT) mass	1243:1285	The OO group had the highest brown adipose tissue (BAT) mass while the SBO group had higher Il6 and lower Cpt1a expression in white adipose tissue (WAT) than other HFD groups.
30460700	6	2	theme	adipose	1261:1267	arg1	BAT					1277:1279	BAT	1277:1279	BAT	1277:1279	The OO group had the highest brown adipose tissue (BAT) mass while the SBO group had higher Il6 and lower Cpt1a expression in white adipose tissue (WAT) than other HFD groups.
30460700	8	3	theme	inflammatory	1606:1617	arg1	response					1619:1626	inflammatory response	1606:1626	inflammatory response to LPS	1606:1633	However, the diets did not have differential effects on inflammatory response to LPS.
30460700	4	4	theme	25	804:805	arg1	%					806:806	%	806:806	%	806:806	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	4	5	theme	olive	924:928	arg1	oil					930:932	olive oil	924:932	olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO])	924:986	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	4	6	theme	Male	606:609	arg1	mice					619:622	Male C57BL/6 mice	606:622	Male C57BL/6 mice	606:622	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	6	7	theme	lower	1326:1330	arg1	expression					1338:1347	lower Cpt1a expression	1326:1347	lower Cpt1a expression in white adipose tissue (WAT)	1326:1377	The OO group had the highest brown adipose tissue (BAT) mass while the SBO group had higher Il6 and lower Cpt1a expression in white adipose tissue (WAT) than other HFD groups.
30460700	4	8	theme	high-fat	772:779	arg1	diets					781:785	three high-fat diets	766:785	three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil	766:1002	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	4	8	theme	high-fat	772:779	arg1	HFD					788:790	HFD	788:790	HFD	788:790	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	9	9	theme	18-C	1686:1689	arg1	acids					1697:1701	18-C fatty acids	1686:1701	18-C fatty acids	1686:1701	These data indicate that the saturation degree of 18-C fatty acids is not an important factor on response to LPS with regard to metabolic and inflammatory indicators.
30460700	4	10	theme	diets	659:663	arg1	diets					659:663	the four different diets	640:663	the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum	640:1070	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	4	10	theme	diets	659:663	arg1	one					633:635	one	633:635	one	633:635	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	2	11	theme	fatty-acid	275:284	arg1	class					286:290	the largest dietary fatty-acid class	255:290	the largest dietary fatty-acid class	255:290	However, despite being the largest dietary fatty-acid class, effects of 18-carbon fatty acids with different degrees of saturation on inflammatory, metabolic, and thermogenic markers have not been well studied.
30460700	9	12	theme	acids	1697:1701	arg1	factor					1723:1728	an important factor	1710:1728	an important factor	1710:1728	These data indicate that the saturation degree of 18-C fatty acids is not an important factor on response to LPS with regard to metabolic and inflammatory indicators.
30460700	9	12	theme	acids	1697:1701	arg1	degree					1676:1681	the saturation degree	1661:1681	the saturation degree of 18-C fatty acids	1661:1701	These data indicate that the saturation degree of 18-C fatty acids is not an important factor on response to LPS with regard to metabolic and inflammatory indicators.
30460700	0	13	with	Diets	97:101	arg1	Composition					138:148	Distinct 18-Carbon Fatty-Acid Composition	108:148	Distinct 18-Carbon Fatty-Acid Composition	108:148	Lipopolysaccharide Alters Thermogenic and Inflammatory Genes in White Adipose Tissue in Mice Fed Diets with Distinct 18-Carbon Fatty-Acid Composition.
30460700	2	14	theme	fatty	314:318	arg1	acids					320:324	18-carbon fatty acids	304:324	18-carbon fatty acids with different degrees of saturation	304:361	However, despite being the largest dietary fatty-acid class, effects of 18-carbon fatty acids with different degrees of saturation on inflammatory, metabolic, and thermogenic markers have not been well studied.
30460700	4	15	dep	fat	736:738	arg1	fat					1037:1039	polyunsaturated fatty-acid-rich fat	1005:1039	polyunsaturated fatty-acid-rich fat; soybean oil [SBO	1005:1057	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	0	16	theme	18-Carbon	117:125	arg1	Composition					138:148	Distinct 18-Carbon Fatty-Acid Composition	108:148	Distinct 18-Carbon Fatty-Acid Composition	108:148	Lipopolysaccharide Alters Thermogenic and Inflammatory Genes in White Adipose Tissue in Mice Fed Diets with Distinct 18-Carbon Fatty-Acid Composition.
30460700	4	17	dep	fat	1037:1039	arg1	[SBO					1054:1057	soybean oil [SBO	1042:1057	polyunsaturated fatty-acid-rich fat; soybean oil [SBO	1005:1057	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	1	18	theme	responses	210:218	arg1	regulation					183:192	the regulation	179:192	the regulation of inflammatory responses in obesity	179:229	Fatty acids are involved in the regulation of inflammatory responses in obesity.
30460700	4	19	theme	%	833:833	arg1	fat					840:842	20% kcal fat	831:842	20% kcal fat	831:842	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	9	20	theme	important	1713:1721	arg1	factor					1723:1728	an important factor	1710:1728	an important factor	1710:1728	These data indicate that the saturation degree of 18-C fatty acids is not an important factor on response to LPS with regard to metabolic and inflammatory indicators.
30460700	9	20	theme	important	1713:1721	arg1	degree					1676:1681	the saturation degree	1661:1681	the saturation degree of 18-C fatty acids	1661:1701	These data indicate that the saturation degree of 18-C fatty acids is not an important factor on response to LPS with regard to metabolic and inflammatory indicators.
30460700	4	21	theme	kcal	808:811	arg1	fat					813:815	25% kcal fat	804:815	25% kcal fat	804:815	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	4	22	theme	fatty-acid-rich	1021:1035	arg1	fat					1037:1039	polyunsaturated fatty-acid-rich fat	1005:1039	polyunsaturated fatty-acid-rich fat; soybean oil [SBO	1005:1057	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	5	23	contain	had	1181:1183	arg2	accumulation					1212:1223	fat accumulation	1208:1223	fat accumulation	1208:1223	Compared to CON, HFD-fed mice had higher weight gain and fat accumulation.
30460700	5	23	contain	had	1181:1183	arg2	gain					1199:1202	higher weight gain	1185:1202	higher weight gain	1185:1202	Compared to CON, HFD-fed mice had higher weight gain and fat accumulation.
30460700	5	23	contain	had	1181:1183	arg1	mice					1176:1179	HFD-fed mice	1168:1179	HFD-fed mice	1168:1179	Compared to CON, HFD-fed mice had higher weight gain and fat accumulation.
30460700	4	24	dep	oil	868:870	arg1	fat					899:901	saturated fatty-acid-rich fat	873:901	saturated fatty-acid-rich fat; shea butter [SHB	873:919	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	7	25	theme	thermogenic	1521:1531	arg1	expression					1538:1547	thermogenic gene expression	1521:1547	thermogenic gene expression	1521:1547	Treatment with LPS upregulated expression of proinflammatory cytokines, and this was associated with downregulation of thermogenic gene expression.
30460700	4	26	theme	%	701:701	arg1	fat					708:710	5.6% kcal fat	698:710	5.6% kcal fat	698:710	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	6	27	theme	adipose	1358:1364	arg1	tissue					1366:1371	white adipose tissue	1352:1371	white adipose tissue (WAT)	1352:1377	The OO group had the highest brown adipose tissue (BAT) mass while the SBO group had higher Il6 and lower Cpt1a expression in white adipose tissue (WAT) than other HFD groups.
30460700	2	28	theme	largest	259:265	arg1	class					286:290	the largest dietary fatty-acid class	255:290	the largest dietary fatty-acid class	255:290	However, despite being the largest dietary fatty-acid class, effects of 18-carbon fatty acids with different degrees of saturation on inflammatory, metabolic, and thermogenic markers have not been well studied.
30460700	4	29	from	oil	753:755	arg1	fat					736:738	4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil	726:1002	4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO])	726:1059	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	4	29	from	oil	753:755	arg1	lard					717:720	lard	717:720	lard	717:720	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	2	30	from	effects	293:299	arg1	markers					407:413	inflammatory, metabolic, and thermogenic markers	366:413	inflammatory, metabolic, and thermogenic markers	366:413	However, despite being the largest dietary fatty-acid class, effects of 18-carbon fatty acids with different degrees of saturation on inflammatory, metabolic, and thermogenic markers have not been well studied.
30460700	4	31	theme	butter	861:866	arg1	oil					868:870	shea butter oil	856:870	shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB])	856:921	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	4	32	theme	soybean	992:998	arg1	oil					1000:1002	soybean oil	992:1002	soybean oil	992:1002	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	0	33	theme	Adipose	70:76	arg1	Tissue					78:83	White Adipose Tissue	64:83	White Adipose Tissue in Mice Fed Diets with Distinct 18-Carbon Fatty-Acid Composition	64:148	Lipopolysaccharide Alters Thermogenic and Inflammatory Genes in White Adipose Tissue in Mice Fed Diets with Distinct 18-Carbon Fatty-Acid Composition.
30460700	4	34	contain	containing	793:802	arg1	diets					781:785	three high-fat diets	766:785	three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil	766:1002	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	4	34	contain	containing	793:802	arg1	HFD					788:790	HFD	788:790	HFD	788:790	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	4	34	contain	containing	793:802	arg2	fat					813:815	25% kcal fat	804:815	25% kcal fat	804:815	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	4	35	dep	oil	930:932	arg1	fat					967:969	monounsaturated fatty-acid-rich fat	935:969	monounsaturated fatty-acid-rich fat; olive oil [OO	935:984	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	4	36	theme	ad	1061:1062	arg1	libitum					1064:1070	ad libitum	1061:1070	ad libitum	1061:1070	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	1	37	from	regulation	183:192	arg1	obesity					223:229	obesity	223:229	obesity	223:229	Fatty acids are involved in the regulation of inflammatory responses in obesity.
30460700	6	38	theme	white	1352:1356	arg1	tissue					1366:1371	white adipose tissue	1352:1371	white adipose tissue (WAT)	1352:1377	The OO group had the highest brown adipose tissue (BAT) mass while the SBO group had higher Il6 and lower Cpt1a expression in white adipose tissue (WAT) than other HFD groups.
30460700	4	39	theme	kcal	731:734	arg1	fat					736:738	4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil	726:1002	4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO])	726:1059	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	2	40	theme	thermogenic	395:405	arg1	markers					407:413	inflammatory, metabolic, and thermogenic markers	366:413	inflammatory, metabolic, and thermogenic markers	366:413	However, despite being the largest dietary fatty-acid class, effects of 18-carbon fatty acids with different degrees of saturation on inflammatory, metabolic, and thermogenic markers have not been well studied.
30460700	4	41	dep	fat	967:969	arg1	oil					978:980	olive oil [OO	972:984	monounsaturated fatty-acid-rich fat; olive oil [OO	935:984	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	7	42	theme	proinflammatory	1447:1461	arg1	cytokines					1463:1471	proinflammatory cytokines	1447:1471	proinflammatory cytokines	1447:1471	Treatment with LPS upregulated expression of proinflammatory cytokines, and this was associated with downregulation of thermogenic gene expression.
30460700	4	43	theme	butter	909:914	arg1	[SHB					916:919	shea butter [SHB	904:919	saturated fatty-acid-rich fat; shea butter [SHB	873:919	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	4	44	theme	oil	1050:1052	arg1	[SBO					1054:1057	soybean oil [SBO	1042:1057	polyunsaturated fatty-acid-rich fat; soybean oil [SBO	1005:1057	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	6	45	contain	had	1307:1309	arg1	group					1301:1305	the SBO group	1293:1305	the SBO group	1293:1305	The OO group had the highest brown adipose tissue (BAT) mass while the SBO group had higher Il6 and lower Cpt1a expression in white adipose tissue (WAT) than other HFD groups.
30460700	6	45	contain	had	1307:1309	arg2	Il6					1318:1320	higher Il6	1311:1320	higher Il6	1311:1320	The OO group had the highest brown adipose tissue (BAT) mass while the SBO group had higher Il6 and lower Cpt1a expression in white adipose tissue (WAT) than other HFD groups.
30460700	6	45	contain	had	1307:1309	arg2	expression					1338:1347	lower Cpt1a expression	1326:1347	lower Cpt1a expression in white adipose tissue (WAT)	1326:1377	The OO group had the highest brown adipose tissue (BAT) mass while the SBO group had higher Il6 and lower Cpt1a expression in white adipose tissue (WAT) than other HFD groups.
30460700	7	46	with	Treatment	1402:1410	arg1	LPS					1417:1419	LPS	1417:1419	LPS	1417:1419	Treatment with LPS upregulated expression of proinflammatory cytokines, and this was associated with downregulation of thermogenic gene expression.
30460700	0	47	theme	Thermogenic	26:36	arg1	Genes					55:59	Thermogenic and Inflammatory Genes	26:59	Thermogenic and Inflammatory Genes	26:59	Lipopolysaccharide Alters Thermogenic and Inflammatory Genes in White Adipose Tissue in Mice Fed Diets with Distinct 18-Carbon Fatty-Acid Composition.
30460700	1	48	theme	Fatty	151:155	arg1	acids					157:161	Fatty acids	151:161	Fatty acids	151:161	Fatty acids are involved in the regulation of inflammatory responses in obesity.
30460700	4	49	theme	fatty-acid-rich	883:897	arg1	fat					899:901	saturated fatty-acid-rich fat	873:901	saturated fatty-acid-rich fat; shea butter [SHB	873:919	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	4	50	theme	terminal	1102:1109	arg1	LPS					1135:1137	LPS	1135:1137	LPS	1135:1137	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	4	50	theme	terminal	1102:1109	arg1	lipopolysaccharide					1115:1132	a terminal 4-h lipopolysaccharide	1100:1132	a terminal 4-h lipopolysaccharide (LPS) treatment	1100:1148	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	0	51	theme	Inflammatory	42:53	arg1	Genes					55:59	Thermogenic and Inflammatory Genes	26:59	Thermogenic and Inflammatory Genes	26:59	Lipopolysaccharide Alters Thermogenic and Inflammatory Genes in White Adipose Tissue in Mice Fed Diets with Distinct 18-Carbon Fatty-Acid Composition.
30460700	4	52	theme	lipopolysaccharide	1115:1132	arg1	treatment					1140:1148	a terminal 4-h lipopolysaccharide (LPS) treatment	1100:1148	a terminal 4-h lipopolysaccharide (LPS) treatment	1100:1148	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	4	53	theme	monounsaturated	935:949	arg1	fat					967:969	monounsaturated fatty-acid-rich fat	935:969	monounsaturated fatty-acid-rich fat; olive oil [OO	935:984	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	5	54	theme	fat	1208:1210	arg1	accumulation					1212:1223	fat accumulation	1208:1223	fat accumulation	1208:1223	Compared to CON, HFD-fed mice had higher weight gain and fat accumulation.
30460700	2	55	theme	different	331:339	arg1	degrees					341:347	different degrees	331:347	different degrees of saturation	331:361	However, despite being the largest dietary fatty-acid class, effects of 18-carbon fatty acids with different degrees of saturation on inflammatory, metabolic, and thermogenic markers have not been well studied.
30460700	3	56	theme	18-carbon	518:526	arg1	profiles					539:546	different 18-carbon fatty-acid profiles	508:546	different 18-carbon fatty-acid profiles	508:546	Therefore, the objective of this study was to test if diets with different 18-carbon fatty-acid profiles differentially regulate inflammatory and metabolic genes.
30460700	4	57	theme	C57BL/6	611:617	arg1	mice					619:622	Male C57BL/6 mice	606:622	Male C57BL/6 mice	606:622	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	5	58	theme	higher	1185:1190	arg1	gain					1199:1202	higher weight gain	1185:1202	higher weight gain	1185:1202	Compared to CON, HFD-fed mice had higher weight gain and fat accumulation.
30460700	6	59	theme	tissue	1269:1274	arg1	mass					1282:1285	the highest brown adipose tissue (BAT) mass	1243:1285	the highest brown adipose tissue (BAT) mass	1243:1285	The OO group had the highest brown adipose tissue (BAT) mass while the SBO group had higher Il6 and lower Cpt1a expression in white adipose tissue (WAT) than other HFD groups.
30460700	9	60	theme	saturation	1665:1674	arg1	factor					1723:1728	an important factor	1710:1728	an important factor	1710:1728	These data indicate that the saturation degree of 18-C fatty acids is not an important factor on response to LPS with regard to metabolic and inflammatory indicators.
30460700	9	60	theme	saturation	1665:1674	arg1	degree					1676:1681	the saturation degree	1661:1681	the saturation degree of 18-C fatty acids	1661:1701	These data indicate that the saturation degree of 18-C fatty acids is not an important factor on response to LPS with regard to metabolic and inflammatory indicators.
30460700	3	61	with	diets	497:501	arg1	profiles					539:546	different 18-carbon fatty-acid profiles	508:546	different 18-carbon fatty-acid profiles	508:546	Therefore, the objective of this study was to test if diets with different 18-carbon fatty-acid profiles differentially regulate inflammatory and metabolic genes.
30460700	3	62	theme	fatty-acid	528:537	arg1	profiles					539:546	different 18-carbon fatty-acid profiles	508:546	different 18-carbon fatty-acid profiles	508:546	Therefore, the objective of this study was to test if diets with different 18-carbon fatty-acid profiles differentially regulate inflammatory and metabolic genes.
30460700	6	63	theme	brown	1255:1259	arg1	tissue					1269:1274	brown adipose tissue	1255:1274	the highest brown adipose tissue (BAT) mass	1243:1285	The OO group had the highest brown adipose tissue (BAT) mass while the SBO group had higher Il6 and lower Cpt1a expression in white adipose tissue (WAT) than other HFD groups.
30460700	6	63	theme	brown	1255:1259	arg1	BAT					1277:1279	BAT	1277:1279	BAT	1277:1279	The OO group had the highest brown adipose tissue (BAT) mass while the SBO group had higher Il6 and lower Cpt1a expression in white adipose tissue (WAT) than other HFD groups.
30460700	8	64	contain	have	1577:1580	arg1	diets					1563:1567	the diets	1559:1567	the diets	1559:1567	However, the diets did not have differential effects on inflammatory response to LPS.
30460700	8	64	contain	have	1577:1580	arg2	effects					1595:1601	differential effects	1582:1601	differential effects	1582:1601	However, the diets did not have differential effects on inflammatory response to LPS.
30460700	2	65	theme	acids	320:324	arg1	effects					293:299	effects	293:299	effects of 18-carbon fatty acids with different degrees of saturation on inflammatory, metabolic, and thermogenic markers	293:413	However, despite being the largest dietary fatty-acid class, effects of 18-carbon fatty acids with different degrees of saturation on inflammatory, metabolic, and thermogenic markers have not been well studied.
30460700	3	66	theme	different	508:516	arg1	profiles					539:546	different 18-carbon fatty-acid profiles	508:546	different 18-carbon fatty-acid profiles	508:546	Therefore, the objective of this study was to test if diets with different 18-carbon fatty-acid profiles differentially regulate inflammatory and metabolic genes.
30460700	9	67	theme	fatty	1691:1695	arg1	acids					1697:1701	18-C fatty acids	1686:1701	18-C fatty acids	1686:1701	These data indicate that the saturation degree of 18-C fatty acids is not an important factor on response to LPS with regard to metabolic and inflammatory indicators.
30460700	4	68	theme	different	649:657	arg1	diets					659:663	the four different diets	640:663	the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum	640:1070	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	4	69	contain	containing	687:696	arg1	CON					682:684	CON	682:684	CON	682:684	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	4	69	contain	containing	687:696	arg1	diet					676:679	a control diet	666:679	the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum	640:1070	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	4	69	contain	containing	687:696	arg2	fat					708:710	5.6% kcal fat	698:710	5.6% kcal fat	698:710	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	3	70	theme	inflammatory	572:583	arg1	genes					599:603	inflammatory and metabolic genes	572:603	inflammatory and metabolic genes	572:603	Therefore, the objective of this study was to test if diets with different 18-carbon fatty-acid profiles differentially regulate inflammatory and metabolic genes.
30460700	6	71	theme	higher	1311:1316	arg1	Il6					1318:1320	higher Il6	1311:1320	higher Il6	1311:1320	The OO group had the highest brown adipose tissue (BAT) mass while the SBO group had higher Il6 and lower Cpt1a expression in white adipose tissue (WAT) than other HFD groups.
30460700	7	72	theme	expression	1538:1547	arg1	downregulation					1503:1516	downregulation	1503:1516	downregulation of thermogenic gene expression	1503:1547	Treatment with LPS upregulated expression of proinflammatory cytokines, and this was associated with downregulation of thermogenic gene expression.
30460700	6	73	from	expression	1338:1347	arg1	tissue					1366:1371	white adipose tissue	1352:1371	white adipose tissue (WAT)	1352:1377	The OO group had the highest brown adipose tissue (BAT) mass while the SBO group had higher Il6 and lower Cpt1a expression in white adipose tissue (WAT) than other HFD groups.
30460700	4	74	theme	olive	972:976	arg1	oil					978:980	olive oil [OO	972:984	monounsaturated fatty-acid-rich fat; olive oil [OO	935:984	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	0	75	theme	Fatty-Acid	127:136	arg1	Composition					138:148	Distinct 18-Carbon Fatty-Acid Composition	108:148	Distinct 18-Carbon Fatty-Acid Composition	108:148	Lipopolysaccharide Alters Thermogenic and Inflammatory Genes in White Adipose Tissue in Mice Fed Diets with Distinct 18-Carbon Fatty-Acid Composition.
30460700	2	76	theme	18-carbon	304:312	arg1	acids					320:324	18-carbon fatty acids	304:324	18-carbon fatty acids with different degrees of saturation	304:361	However, despite being the largest dietary fatty-acid class, effects of 18-carbon fatty acids with different degrees of saturation on inflammatory, metabolic, and thermogenic markers have not been well studied.
30460700	8	77	theme	differential	1582:1593	arg1	effects					1595:1601	differential effects	1582:1601	differential effects	1582:1601	However, the diets did not have differential effects on inflammatory response to LPS.
30460700	1	78	theme	inflammatory	197:208	arg1	responses					210:218	inflammatory responses	197:218	inflammatory responses in obesity	197:229	Fatty acids are involved in the regulation of inflammatory responses in obesity.
30460700	0	79	from	Tissue	78:83	arg1	Mice					88:91	Mice	88:91	Mice Fed Diets with Distinct 18-Carbon Fatty-Acid Composition	88:148	Lipopolysaccharide Alters Thermogenic and Inflammatory Genes in White Adipose Tissue in Mice Fed Diets with Distinct 18-Carbon Fatty-Acid Composition.
30460700	3	80	theme	metabolic	589:597	arg1	genes					599:603	inflammatory and metabolic genes	572:603	inflammatory and metabolic genes	572:603	Therefore, the objective of this study was to test if diets with different 18-carbon fatty-acid profiles differentially regulate inflammatory and metabolic genes.
30460700	1	81	from	obesity	223:229	arg1	regulation					183:192	the regulation	179:192	the regulation of inflammatory responses in obesity	179:229	Fatty acids are involved in the regulation of inflammatory responses in obesity.
30460700	4	82	dep	lard	717:720	arg1	libitum					1064:1070	ad libitum	1061:1070	ad libitum	1061:1070	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	4	83	theme	%	806:806	arg1	fat					813:815	25% kcal fat	804:815	25% kcal fat	804:815	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	4	84	theme	soybean	1042:1048	arg1	[SBO					1054:1057	soybean oil [SBO	1042:1057	polyunsaturated fatty-acid-rich fat; soybean oil [SBO	1005:1057	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	2	85	theme	dietary	267:273	arg1	class					286:290	the largest dietary fatty-acid class	255:290	the largest dietary fatty-acid class	255:290	However, despite being the largest dietary fatty-acid class, effects of 18-carbon fatty acids with different degrees of saturation on inflammatory, metabolic, and thermogenic markers have not been well studied.
30460700	1	86	from	responses	210:218	arg1	obesity					223:229	obesity	223:229	obesity	223:229	Fatty acids are involved in the regulation of inflammatory responses in obesity.
30460700	7	87	theme	gene	1533:1536	arg1	expression					1538:1547	thermogenic gene expression	1521:1547	thermogenic gene expression	1521:1547	Treatment with LPS upregulated expression of proinflammatory cytokines, and this was associated with downregulation of thermogenic gene expression.
30460700	4	88	theme	5.6	698:700	arg1	%					701:701	%	701:701	%	701:701	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	4	89	from	oil	930:932	arg1	fat					840:842	20% kcal fat	831:842	20% kcal fat	831:842	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	4	89	from	oil	930:932	arg1	lard					822:825	lard	822:825	lard	822:825	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	4	90	theme	shea	856:859	arg1	oil					868:870	shea butter oil	856:870	shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB])	856:921	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	4	91	theme	polyunsaturated	1005:1019	arg1	fat					1037:1039	polyunsaturated fatty-acid-rich fat	1005:1039	polyunsaturated fatty-acid-rich fat; soybean oil [SBO	1005:1057	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	4	92	theme	control	668:674	arg1	CON					682:684	CON	682:684	CON	682:684	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	4	92	theme	control	668:674	arg1	diet					676:679	a control diet	666:679	the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum	640:1070	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	6	93	theme	Cpt1a	1332:1336	arg1	expression					1338:1347	lower Cpt1a expression	1326:1347	lower Cpt1a expression in white adipose tissue (WAT)	1326:1377	The OO group had the highest brown adipose tissue (BAT) mass while the SBO group had higher Il6 and lower Cpt1a expression in white adipose tissue (WAT) than other HFD groups.
30460700	9	94	theme	metabolic	1764:1772	arg1	indicators					1791:1800	metabolic and inflammatory indicators	1764:1800	metabolic and inflammatory indicators	1764:1800	These data indicate that the saturation degree of 18-C fatty acids is not an important factor on response to LPS with regard to metabolic and inflammatory indicators.
30460700	6	95	from	Il6	1318:1320	arg1	tissue					1366:1371	white adipose tissue	1352:1371	white adipose tissue (WAT)	1352:1377	The OO group had the highest brown adipose tissue (BAT) mass while the SBO group had higher Il6 and lower Cpt1a expression in white adipose tissue (WAT) than other HFD groups.
30460700	2	96	with	acids	320:324	arg1	degrees					341:347	different degrees	331:347	different degrees of saturation	331:361	However, despite being the largest dietary fatty-acid class, effects of 18-carbon fatty acids with different degrees of saturation on inflammatory, metabolic, and thermogenic markers have not been well studied.
30460700	4	97	theme	kcal	835:838	arg1	fat					840:842	20% kcal fat	831:842	20% kcal fat	831:842	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	9	98	theme	inflammatory	1778:1789	arg1	indicators					1791:1800	metabolic and inflammatory indicators	1764:1800	metabolic and inflammatory indicators	1764:1800	These data indicate that the saturation degree of 18-C fatty acids is not an important factor on response to LPS with regard to metabolic and inflammatory indicators.
30460700	6	99	theme	SBO	1297:1299	arg1	group					1301:1305	the SBO group	1293:1305	the SBO group	1293:1305	The OO group had the highest brown adipose tissue (BAT) mass while the SBO group had higher Il6 and lower Cpt1a expression in white adipose tissue (WAT) than other HFD groups.
30460700	0	100	theme	Distinct	108:115	arg1	Composition					138:148	Distinct 18-Carbon Fatty-Acid Composition	108:148	Distinct 18-Carbon Fatty-Acid Composition	108:148	Lipopolysaccharide Alters Thermogenic and Inflammatory Genes in White Adipose Tissue in Mice Fed Diets with Distinct 18-Carbon Fatty-Acid Composition.
30460700	4	101	from	diets	781:785	arg1	fat					736:738	4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil	726:1002	4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO])	726:1059	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	4	101	from	diets	781:785	arg1	lard					717:720	lard	717:720	lard	717:720	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	4	102	theme	%	729:729	arg1	fat					736:738	4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil	726:1002	4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO])	726:1059	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	6	103	theme	highest	1247:1253	arg1	mass					1282:1285	the highest brown adipose tissue (BAT) mass	1243:1285	the highest brown adipose tissue (BAT) mass	1243:1285	The OO group had the highest brown adipose tissue (BAT) mass while the SBO group had higher Il6 and lower Cpt1a expression in white adipose tissue (WAT) than other HFD groups.
30460700	6	104	theme	other	1384:1388	arg1	groups					1394:1399	other HFD groups	1384:1399	other HFD groups	1384:1399	The OO group had the highest brown adipose tissue (BAT) mass while the SBO group had higher Il6 and lower Cpt1a expression in white adipose tissue (WAT) than other HFD groups.
30460700	7	105	theme	cytokines	1463:1471	arg1	expression					1433:1442	expression	1433:1442	expression of proinflammatory cytokines	1433:1471	Treatment with LPS upregulated expression of proinflammatory cytokines, and this was associated with downregulation of thermogenic gene expression.
30460700	4	106	theme	shea	904:907	arg1	[SHB					916:919	shea butter [SHB	904:919	saturated fatty-acid-rich fat; shea butter [SHB	873:919	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	4	107	theme	kcal	703:706	arg1	fat					708:710	5.6% kcal fat	698:710	5.6% kcal fat	698:710	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	2	108	theme	metabolic	380:388	arg1	markers					407:413	inflammatory, metabolic, and thermogenic markers	366:413	inflammatory, metabolic, and thermogenic markers	366:413	However, despite being the largest dietary fatty-acid class, effects of 18-carbon fatty acids with different degrees of saturation on inflammatory, metabolic, and thermogenic markers have not been well studied.
30460700	4	109	from	oil	1000:1002	arg1	fat					840:842	20% kcal fat	831:842	20% kcal fat	831:842	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	4	109	from	oil	1000:1002	arg1	lard					822:825	lard	822:825	lard	822:825	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	4	110	theme	4-h	1111:1113	arg1	LPS					1135:1137	LPS	1135:1137	LPS	1135:1137	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	4	110	theme	4-h	1111:1113	arg1	lipopolysaccharide					1115:1132	a terminal 4-h lipopolysaccharide	1100:1132	a terminal 4-h lipopolysaccharide (LPS) treatment	1100:1148	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	6	111	theme	OO	1230:1231	arg1	group					1233:1237	The OO group	1226:1237	The OO group	1226:1237	The OO group had the highest brown adipose tissue (BAT) mass while the SBO group had higher Il6 and lower Cpt1a expression in white adipose tissue (WAT) than other HFD groups.
30460700	9	112	from	factor	1723:1728	arg1	response					1733:1740	response	1733:1740	response to LPS with regard to metabolic and inflammatory indicators	1733:1800	These data indicate that the saturation degree of 18-C fatty acids is not an important factor on response to LPS with regard to metabolic and inflammatory indicators.
30460700	4	113	theme	saturated	873:881	arg1	fat					899:901	saturated fatty-acid-rich fat	873:901	saturated fatty-acid-rich fat; shea butter [SHB	873:919	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	5	114	theme	weight	1192:1197	arg1	gain					1199:1202	higher weight gain	1185:1202	higher weight gain	1185:1202	Compared to CON, HFD-fed mice had higher weight gain and fat accumulation.
30460700	0	115	theme	White	64:68	arg1	Tissue					78:83	White Adipose Tissue	64:83	White Adipose Tissue in Mice Fed Diets with Distinct 18-Carbon Fatty-Acid Composition	64:148	Lipopolysaccharide Alters Thermogenic and Inflammatory Genes in White Adipose Tissue in Mice Fed Diets with Distinct 18-Carbon Fatty-Acid Composition.
30460700	6	116	contain	had	1239:1241	arg2	mass					1282:1285	the highest brown adipose tissue (BAT) mass	1243:1285	the highest brown adipose tissue (BAT) mass	1243:1285	The OO group had the highest brown adipose tissue (BAT) mass while the SBO group had higher Il6 and lower Cpt1a expression in white adipose tissue (WAT) than other HFD groups.
30460700	6	116	contain	had	1239:1241	arg1	group					1233:1237	The OO group	1226:1237	The OO group	1226:1237	The OO group had the highest brown adipose tissue (BAT) mass while the SBO group had higher Il6 and lower Cpt1a expression in white adipose tissue (WAT) than other HFD groups.
30460700	6	117	theme	HFD	1390:1392	arg1	groups					1394:1399	other HFD groups	1384:1399	other HFD groups	1384:1399	The OO group had the highest brown adipose tissue (BAT) mass while the SBO group had higher Il6 and lower Cpt1a expression in white adipose tissue (WAT) than other HFD groups.
30460700	5	118	theme	HFD-fed	1168:1174	arg1	mice					1176:1179	HFD-fed mice	1168:1179	HFD-fed mice	1168:1179	Compared to CON, HFD-fed mice had higher weight gain and fat accumulation.
30460700	3	119	theme	study	476:480	arg1	objective					458:466	the objective	454:466	the objective of this study	454:480	Therefore, the objective of this study was to test if diets with different 18-carbon fatty-acid profiles differentially regulate inflammatory and metabolic genes.
30460700	4	120	from	oil	868:870	arg1	fat					840:842	20% kcal fat	831:842	20% kcal fat	831:842	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	4	120	from	oil	868:870	arg1	lard					822:825	lard	822:825	lard	822:825	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	4	121	dep	diets	659:663	arg1	CON					682:684	CON	682:684	CON	682:684	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	4	121	dep	diets	659:663	arg1	diet					676:679	a control diet	666:679	the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum	640:1070	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	4	122	theme	fatty-acid-rich	951:965	arg1	fat					967:969	monounsaturated fatty-acid-rich fat	935:969	monounsaturated fatty-acid-rich fat; olive oil [OO	935:984	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	4	123	theme	soybean	745:751	arg1	CON					758:760	CON	758:760	CON	758:760	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	4	123	theme	soybean	745:751	arg1	oil					753:755	soybean oil	745:755	soybean oil (CON)	745:761	Male C57BL/6 mice were fed one of the four different diets: a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter oil (saturated fatty-acid-rich fat; shea butter [SHB]), olive oil (monounsaturated fatty-acid-rich fat; olive oil [OO]), or soybean oil (polyunsaturated fatty-acid-rich fat; soybean oil [SBO]) ad libitum for 4 weeks with or without a terminal 4-h lipopolysaccharide (LPS) treatment.
30460700	2	124	theme	inflammatory	366:377	arg1	markers					407:413	inflammatory, metabolic, and thermogenic markers	366:413	inflammatory, metabolic, and thermogenic markers	366:413	However, despite being the largest dietary fatty-acid class, effects of 18-carbon fatty acids with different degrees of saturation on inflammatory, metabolic, and thermogenic markers have not been well studied.
29932388	10	0	dep	analyses	1195:1202	arg1	the					1143:1145	the	1143:1145	the	1143:1145	On the basis of phenotypic, genotypic and phylogenetic analyses, strain SJW1-2T (=KACC 19332T=NBRC 112908T) represents a novel species of the genus Deinococcus, for which the name Deinococcus koreensis sp.
29932388	10	0	dep	analyses	1195:1202	arg1	basis					1147:1151	basis	1147:1151	basis	1147:1151	On the basis of phenotypic, genotypic and phylogenetic analyses, strain SJW1-2T (=KACC 19332T=NBRC 112908T) represents a novel species of the genus Deinococcus, for which the name Deinococcus koreensis sp.
29932388	5	1	theme	gamma	658:662	arg1	radiation					664:672	gamma radiation	658:672	gamma radiation	658:672	Strain SJW1-2T exhibited high resistance to gamma radiation.
29932388	2	2	theme	radiation-resistant	105:123	arg1	strain					168:173	A gamma radiation-resistant, Gram-stain-negative, rod-shaped bacterial strain	97:173	A gamma radiation-resistant, Gram-stain-negative, rod-shaped bacterial strain	97:173	A gamma radiation-resistant, Gram-stain-negative, rod-shaped bacterial strain, designated SJW1-2T, was isolated from freshwater samples collected from the Seomjin River, Republic of Korea.
29932388	5	3	theme	high	639:642	arg1	resistance					644:653	high resistance	639:653	high resistance to gamma radiation	639:672	Strain SJW1-2T exhibited high resistance to gamma radiation.
29932388	10	4	theme	phenotypic	1156:1165	arg1	analyses					1195:1202	phenotypic, genotypic and phylogenetic analyses	1156:1202	phenotypic, genotypic and phylogenetic analyses	1156:1202	On the basis of phenotypic, genotypic and phylogenetic analyses, strain SJW1-2T (=KACC 19332T=NBRC 112908T) represents a novel species of the genus Deinococcus, for which the name Deinococcus koreensis sp.
29932388	7	5	theme	polar	725:729	arg1	profile					737:743	The polar lipid profile	721:743	The polar lipid profile	721:743	The polar lipid profile consisted of different unidentified glycolipids, two unidentified lipids, two unidentified phospholipids and an unidentified phosphoglycolipid.
29932388	8	6	theme	peptidoglycan	899:911	arg1	alanine					930:936	alanine	930:936	alanine	930:936	The major peptidoglycan amino acids were alanine, d-glutamic acid, glycine and l-ornithine.
29932388	8	6	theme	peptidoglycan	899:911	arg1	acids					919:923	The major peptidoglycan amino acids	889:923	The major peptidoglycan amino acids	889:923	The major peptidoglycan amino acids were alanine, d-glutamic acid, glycine and l-ornithine.
29932388	10	7	theme	Deinococcus	1288:1298	arg1	species					1267:1273	a novel species	1259:1273	a novel species	1259:1273	On the basis of phenotypic, genotypic and phylogenetic analyses, strain SJW1-2T (=KACC 19332T=NBRC 112908T) represents a novel species of the genus Deinococcus, for which the name Deinococcus koreensis sp.
29932388	1	8	theme	radiation-resistant	40:58	arg1	bacterium					60:68	a gamma radiation-resistant bacterium	32:68	a gamma radiation-resistant bacterium isolated from river water	32:94	nov., a gamma radiation-resistant bacterium isolated from river water.
29932388	1	8	theme	radiation-resistant	40:58	arg1	nov.					26:29	nov.	26:29	nov.	26:29	nov., a gamma radiation-resistant bacterium isolated from river water.
29932388	3	9	dep	rRNA	294:297	arg1	analyses					313:320	gene sequence analyses	299:320	The 16S rRNA gene sequence analyses	286:320	The 16S rRNA gene sequence analyses showed that strain SJW1-2T was most closely related to Deinococcus metalli 1PNM-19T (94.3 % sequence similarity) and formed a robust phylogenetic clade with other species of the genus Deinococcus.
29932388	10	10	theme	Deinococcus	1320:1330	arg1	sp					1342:1343	the name Deinococcus koreensis sp	1311:1343	the name Deinococcus koreensis sp	1311:1343	On the basis of phenotypic, genotypic and phylogenetic analyses, strain SJW1-2T (=KACC 19332T=NBRC 112908T) represents a novel species of the genus Deinococcus, for which the name Deinococcus koreensis sp.
29932388	10	11	theme	genus	1282:1286	arg1	Deinococcus					1288:1298	the genus Deinococcus	1278:1298	the genus Deinococcus	1278:1298	On the basis of phenotypic, genotypic and phylogenetic analyses, strain SJW1-2T (=KACC 19332T=NBRC 112908T) represents a novel species of the genus Deinococcus, for which the name Deinococcus koreensis sp.
29932388	8	12	theme	major	893:897	arg1	alanine					930:936	alanine	930:936	alanine	930:936	The major peptidoglycan amino acids were alanine, d-glutamic acid, glycine and l-ornithine.
29932388	8	12	theme	major	893:897	arg1	acids					919:923	The major peptidoglycan amino acids	889:923	The major peptidoglycan amino acids	889:923	The major peptidoglycan amino acids were alanine, d-glutamic acid, glycine and l-ornithine.
29932388	7	13	theme	unidentified	857:868	arg1	phosphoglycolipid					870:886	an unidentified phosphoglycolipid	854:886	an unidentified phosphoglycolipid	854:886	The polar lipid profile consisted of different unidentified glycolipids, two unidentified lipids, two unidentified phospholipids and an unidentified phosphoglycolipid.
29932388	2	14	theme	bacterial	158:166	arg1	strain					168:173	A gamma radiation-resistant, Gram-stain-negative, rod-shaped bacterial strain	97:173	A gamma radiation-resistant, Gram-stain-negative, rod-shaped bacterial strain	97:173	A gamma radiation-resistant, Gram-stain-negative, rod-shaped bacterial strain, designated SJW1-2T, was isolated from freshwater samples collected from the Seomjin River, Republic of Korea.
29932388	9	15	theme	DNA	1109:1111	arg1	G+C content					1113:1123	the DNA G+C content	1105:1123	the DNA G+C content	1105:1123	The predominant fatty acids (>10 %) were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) (25.2 %) and C16 : 0 (21.2 %), and the DNA G+C content was 69.5 mol%.
29932388	9	15	theme	DNA	1109:1111	arg1	%					1137:1137	69.5 mol%	1129:1137	69.5 mol%	1129:1137	The predominant fatty acids (>10 %) were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) (25.2 %) and C16 : 0 (21.2 %), and the DNA G+C content was 69.5 mol%.
29932388	10	16	theme	name	1315:1318	arg1	sp					1342:1343	the name Deinococcus koreensis sp	1311:1343	the name Deinococcus koreensis sp	1311:1343	On the basis of phenotypic, genotypic and phylogenetic analyses, strain SJW1-2T (=KACC 19332T=NBRC 112908T) represents a novel species of the genus Deinococcus, for which the name Deinococcus koreensis sp.
29932388	0	17	theme	Deinococcus	0:10	arg1	sp					22:23	Deinococcus koreensis sp	0:23	Deinococcus koreensis sp.	0:24	Deinococcus koreensis sp.
29932388	6	18	theme	respiratory	691:701	arg1	MK-8					715:718	MK-8	715:718	MK-8	715:718	The predominant respiratory quinone was MK-8.
29932388	6	18	theme	respiratory	691:701	arg1	quinone					703:709	The predominant respiratory quinone	675:709	The predominant respiratory quinone	675:709	The predominant respiratory quinone was MK-8.
29932388	7	19	theme	different	758:766	arg1	glycolipids					781:791	different unidentified glycolipids	758:791	different unidentified glycolipids	758:791	The polar lipid profile consisted of different unidentified glycolipids, two unidentified lipids, two unidentified phospholipids and an unidentified phosphoglycolipid.
29932388	3	20	theme	Deinococcus	377:387	arg1	similarity					423:432	94.3 % sequence similarity	407:432	94.3 % sequence similarity	407:432	The 16S rRNA gene sequence analyses showed that strain SJW1-2T was most closely related to Deinococcus metalli 1PNM-19T (94.3 % sequence similarity) and formed a robust phylogenetic clade with other species of the genus Deinococcus.
29932388	3	20	theme	Deinococcus	377:387	arg1	1PNM-19T					397:404	Deinococcus metalli 1PNM-19T	377:404	Deinococcus metalli 1PNM-19T (94.3 % sequence similarity)	377:433	The 16S rRNA gene sequence analyses showed that strain SJW1-2T was most closely related to Deinococcus metalli 1PNM-19T (94.3 % sequence similarity) and formed a robust phylogenetic clade with other species of the genus Deinococcus.
29932388	7	21	theme	unidentified	798:809	arg1	lipids					811:816	two unidentified lipids	794:816	two unidentified lipids	794:816	The polar lipid profile consisted of different unidentified glycolipids, two unidentified lipids, two unidentified phospholipids and an unidentified phosphoglycolipid.
29932388	5	22	theme	Strain	614:619	arg1	SJW1-2T					621:627	Strain SJW1-2T	614:627	Strain SJW1-2T	614:627	Strain SJW1-2T exhibited high resistance to gamma radiation.
29932388	1	23	theme	river	84:88	arg1	water					90:94	river water	84:94	river water	84:94	nov., a gamma radiation-resistant bacterium isolated from river water.
29932388	6	24	theme	predominant	679:689	arg1	MK-8					715:718	MK-8	715:718	MK-8	715:718	The predominant respiratory quinone was MK-8.
29932388	6	24	theme	predominant	679:689	arg1	quinone					703:709	The predominant respiratory quinone	675:709	The predominant respiratory quinone	675:709	The predominant respiratory quinone was MK-8.
29932388	3	25	theme	sequence	414:421	arg1	similarity					423:432	94.3 % sequence similarity	407:432	94.3 % sequence similarity	407:432	The 16S rRNA gene sequence analyses showed that strain SJW1-2T was most closely related to Deinococcus metalli 1PNM-19T (94.3 % sequence similarity) and formed a robust phylogenetic clade with other species of the genus Deinococcus.
29932388	3	25	theme	sequence	414:421	arg1	1PNM-19T					397:404	Deinococcus metalli 1PNM-19T	377:404	Deinococcus metalli 1PNM-19T (94.3 % sequence similarity)	377:433	The 16S rRNA gene sequence analyses showed that strain SJW1-2T was most closely related to Deinococcus metalli 1PNM-19T (94.3 % sequence similarity) and formed a robust phylogenetic clade with other species of the genus Deinococcus.
29932388	3	26	theme	metalli	389:395	arg1	similarity					423:432	94.3 % sequence similarity	407:432	94.3 % sequence similarity	407:432	The 16S rRNA gene sequence analyses showed that strain SJW1-2T was most closely related to Deinococcus metalli 1PNM-19T (94.3 % sequence similarity) and formed a robust phylogenetic clade with other species of the genus Deinococcus.
29932388	3	26	theme	metalli	389:395	arg1	1PNM-19T					397:404	Deinococcus metalli 1PNM-19T	377:404	Deinococcus metalli 1PNM-19T (94.3 % sequence similarity)	377:433	The 16S rRNA gene sequence analyses showed that strain SJW1-2T was most closely related to Deinococcus metalli 1PNM-19T (94.3 % sequence similarity) and formed a robust phylogenetic clade with other species of the genus Deinococcus.
29932388	9	27	theme	69.5 mol	1129:1136	arg1	G+C content					1113:1123	the DNA G+C content	1105:1123	the DNA G+C content	1105:1123	The predominant fatty acids (>10 %) were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) (25.2 %) and C16 : 0 (21.2 %), and the DNA G+C content was 69.5 mol%.
29932388	9	27	theme	69.5 mol	1129:1136	arg1	%					1137:1137	69.5 mol%	1129:1137	69.5 mol%	1129:1137	The predominant fatty acids (>10 %) were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) (25.2 %) and C16 : 0 (21.2 %), and the DNA G+C content was 69.5 mol%.
29932388	10	28	theme	=KACC	1221:1225	arg1	SJW1-2T					1212:1218	strain SJW1-2T	1205:1218	strain SJW1-2T (=KACC 19332T=NBRC 112908T)	1205:1246	On the basis of phenotypic, genotypic and phylogenetic analyses, strain SJW1-2T (=KACC 19332T=NBRC 112908T) represents a novel species of the genus Deinococcus, for which the name Deinococcus koreensis sp.
29932388	10	28	theme	=KACC	1221:1225	arg1	112908T					1239:1245	=KACC 19332T=NBRC 112908T	1221:1245	=KACC 19332T=NBRC 112908T	1221:1245	On the basis of phenotypic, genotypic and phylogenetic analyses, strain SJW1-2T (=KACC 19332T=NBRC 112908T) represents a novel species of the genus Deinococcus, for which the name Deinococcus koreensis sp.
29932388	2	29	attach	isolated	200:207	arg2	strain					168:173	A gamma radiation-resistant, Gram-stain-negative, rod-shaped bacterial strain	97:173	A gamma radiation-resistant, Gram-stain-negative, rod-shaped bacterial strain	97:173	A gamma radiation-resistant, Gram-stain-negative, rod-shaped bacterial strain, designated SJW1-2T, was isolated from freshwater samples collected from the Seomjin River, Republic of Korea.
29932388	2	29	attach	isolated	200:207	arg1	samples					225:231	freshwater samples	214:231	freshwater samples collected from the Seomjin River, Republic of Korea	214:283	A gamma radiation-resistant, Gram-stain-negative, rod-shaped bacterial strain, designated SJW1-2T, was isolated from freshwater samples collected from the Seomjin River, Republic of Korea.
29932388	9	30	dep	C16 	1040:1043	arg1	 1ω6c					1063:1067	 1ω6c	1063:1067	 1ω6c	1063:1067	The predominant fatty acids (>10 %) were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) (25.2 %) and C16 : 0 (21.2 %), and the DNA G+C content was 69.5 mol%.
29932388	9	30	dep	C16 	1040:1043	arg1	C16 					1058:1061	C16 	1058:1061	C16 	1058:1061	The predominant fatty acids (>10 %) were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) (25.2 %) and C16 : 0 (21.2 %), and the DNA G+C content was 69.5 mol%.
29932388	9	30	dep	C16 	1040:1043	arg1	 1ω7c					1045:1049	 1ω7c	1045:1049	 1ω7c	1045:1049	The predominant fatty acids (>10 %) were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) (25.2 %) and C16 : 0 (21.2 %), and the DNA G+C content was 69.5 mol%.
29932388	9	31	dep	feature	1029:1035	arg1	3					1037:1037	3	1037:1037	3	1037:1037	The predominant fatty acids (>10 %) were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) (25.2 %) and C16 : 0 (21.2 %), and the DNA G+C content was 69.5 mol%.
29932388	9	31	dep	feature	1029:1035	arg1	%					1097:1097	21.2 %	1092:1097	21.2 %	1092:1097	The predominant fatty acids (>10 %) were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) (25.2 %) and C16 : 0 (21.2 %), and the DNA G+C content was 69.5 mol%.
29932388	9	31	dep	feature	1029:1035	arg1	 0					1088:1089	 0	1088:1089	 0	1088:1089	The predominant fatty acids (>10 %) were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) (25.2 %) and C16 : 0 (21.2 %), and the DNA G+C content was 69.5 mol%.
29932388	9	31	dep	feature	1029:1035	arg1	%					1076:1076	25.2 %	1071:1076	25.2 %	1071:1076	The predominant fatty acids (>10 %) were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) (25.2 %) and C16 : 0 (21.2 %), and the DNA G+C content was 69.5 mol%.
29932388	9	31	dep	feature	1029:1035	arg1	C16 					1040:1043	C16 	1040:1043	C16 	1040:1043	The predominant fatty acids (>10 %) were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) (25.2 %) and C16 : 0 (21.2 %), and the DNA G+C content was 69.5 mol%.
29932388	3	32	theme	robust	448:453	arg1	clade					468:472	a robust phylogenetic clade	446:472	a robust phylogenetic clade	446:472	The 16S rRNA gene sequence analyses showed that strain SJW1-2T was most closely related to Deinococcus metalli 1PNM-19T (94.3 % sequence similarity) and formed a robust phylogenetic clade with other species of the genus Deinococcus.
29932388	10	33	theme	strain	1205:1210	arg1	SJW1-2T					1212:1218	strain SJW1-2T	1205:1218	strain SJW1-2T (=KACC 19332T=NBRC 112908T)	1205:1246	On the basis of phenotypic, genotypic and phylogenetic analyses, strain SJW1-2T (=KACC 19332T=NBRC 112908T) represents a novel species of the genus Deinococcus, for which the name Deinococcus koreensis sp.
29932388	10	33	theme	strain	1205:1210	arg1	112908T					1239:1245	=KACC 19332T=NBRC 112908T	1221:1245	=KACC 19332T=NBRC 112908T	1221:1245	On the basis of phenotypic, genotypic and phylogenetic analyses, strain SJW1-2T (=KACC 19332T=NBRC 112908T) represents a novel species of the genus Deinococcus, for which the name Deinococcus koreensis sp.
29932388	3	34	theme	phylogenetic	455:466	arg1	clade					468:472	a robust phylogenetic clade	446:472	a robust phylogenetic clade	446:472	The 16S rRNA gene sequence analyses showed that strain SJW1-2T was most closely related to Deinococcus metalli 1PNM-19T (94.3 % sequence similarity) and formed a robust phylogenetic clade with other species of the genus Deinococcus.
29932388	3	35	theme	94.3 	407:411	arg1	%					412:412	%	412:412	%	412:412	The 16S rRNA gene sequence analyses showed that strain SJW1-2T was most closely related to Deinococcus metalli 1PNM-19T (94.3 % sequence similarity) and formed a robust phylogenetic clade with other species of the genus Deinococcus.
29932388	1	36	attach	isolated	70:77	arg1	water					90:94	river water	84:94	river water	84:94	nov., a gamma radiation-resistant bacterium isolated from river water.
29932388	1	36	attach	isolated	70:77	arg2	nov.					26:29	nov.	26:29	nov.	26:29	nov., a gamma radiation-resistant bacterium isolated from river water.
29932388	1	36	attach	isolated	70:77	arg2	bacterium					60:68	a gamma radiation-resistant bacterium	32:68	a gamma radiation-resistant bacterium isolated from river water	32:94	nov., a gamma radiation-resistant bacterium isolated from river water.
29932388	7	37	theme	unidentified	823:834	arg1	phospholipids					836:848	two unidentified phospholipids	819:848	two unidentified phospholipids	819:848	The polar lipid profile consisted of different unidentified glycolipids, two unidentified lipids, two unidentified phospholipids and an unidentified phosphoglycolipid.
29932388	3	38	theme	%	412:412	arg1	similarity					423:432	94.3 % sequence similarity	407:432	94.3 % sequence similarity	407:432	The 16S rRNA gene sequence analyses showed that strain SJW1-2T was most closely related to Deinococcus metalli 1PNM-19T (94.3 % sequence similarity) and formed a robust phylogenetic clade with other species of the genus Deinococcus.
29932388	3	38	theme	%	412:412	arg1	1PNM-19T					397:404	Deinococcus metalli 1PNM-19T	377:404	Deinococcus metalli 1PNM-19T (94.3 % sequence similarity)	377:433	The 16S rRNA gene sequence analyses showed that strain SJW1-2T was most closely related to Deinococcus metalli 1PNM-19T (94.3 % sequence similarity) and formed a robust phylogenetic clade with other species of the genus Deinococcus.
29932388	2	39	dep	radiation-resistant	105:123	arg1	rod-shaped					147:156	rod-shaped	147:156	rod-shaped	147:156	A gamma radiation-resistant, Gram-stain-negative, rod-shaped bacterial strain, designated SJW1-2T, was isolated from freshwater samples collected from the Seomjin River, Republic of Korea.
29932388	2	39	dep	radiation-resistant	105:123	arg1	Gram-stain-negative					126:144	Gram-stain-negative	126:144	Gram-stain-negative	126:144	A gamma radiation-resistant, Gram-stain-negative, rod-shaped bacterial strain, designated SJW1-2T, was isolated from freshwater samples collected from the Seomjin River, Republic of Korea.
29932388	10	40	dep	Deinococcus	1320:1330	arg1	koreensis					1332:1340	koreensis	1332:1340	koreensis	1332:1340	On the basis of phenotypic, genotypic and phylogenetic analyses, strain SJW1-2T (=KACC 19332T=NBRC 112908T) represents a novel species of the genus Deinococcus, for which the name Deinococcus koreensis sp.
29932388	7	41	theme	unidentified	768:779	arg1	glycolipids					781:791	different unidentified glycolipids	758:791	different unidentified glycolipids	758:791	The polar lipid profile consisted of different unidentified glycolipids, two unidentified lipids, two unidentified phospholipids and an unidentified phosphoglycolipid.
29932388	10	42	theme	phylogenetic	1182:1193	arg1	analyses					1195:1202	phenotypic, genotypic and phylogenetic analyses	1156:1202	phenotypic, genotypic and phylogenetic analyses	1156:1202	On the basis of phenotypic, genotypic and phylogenetic analyses, strain SJW1-2T (=KACC 19332T=NBRC 112908T) represents a novel species of the genus Deinococcus, for which the name Deinococcus koreensis sp.
29932388	3	43	theme	gene	299:302	arg1	analyses					313:320	gene sequence analyses	299:320	The 16S rRNA gene sequence analyses	286:320	The 16S rRNA gene sequence analyses showed that strain SJW1-2T was most closely related to Deinococcus metalli 1PNM-19T (94.3 % sequence similarity) and formed a robust phylogenetic clade with other species of the genus Deinococcus.
29932388	8	44	theme	d-glutamic	939:948	arg1	acid					950:953	d-glutamic acid	939:953	d-glutamic acid	939:953	The major peptidoglycan amino acids were alanine, d-glutamic acid, glycine and l-ornithine.
29932388	3	45	theme	other	479:483	arg1	species					485:491	other species	479:491	other species of the genus Deinococcus	479:516	The 16S rRNA gene sequence analyses showed that strain SJW1-2T was most closely related to Deinococcus metalli 1PNM-19T (94.3 % sequence similarity) and formed a robust phylogenetic clade with other species of the genus Deinococcus.
29932388	9	46	theme	predominant	985:995	arg1	acids					1003:1007	The predominant fatty acids	981:1007	The predominant fatty acids (>10 %)	981:1015	The predominant fatty acids (>10 %) were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) (25.2 %) and C16 : 0 (21.2 %), and the DNA G+C content was 69.5 mol%.
29932388	9	46	theme	predominant	985:995	arg1	%					1014:1014	>10 %	1010:1014	>10 %	1010:1014	The predominant fatty acids (>10 %) were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) (25.2 %) and C16 : 0 (21.2 %), and the DNA G+C content was 69.5 mol%.
29932388	4	47	theme	growth	531:536	arg1	pH					578:579	pH 7.0-7.5	578:587	pH 7.0-7.5	578:587	The optimum growth pH and temperature for the isolate were pH 7.0-7.5 and 25 °C, respectively.
29932388	4	47	theme	growth	531:536	arg1	pH					538:539	The optimum growth pH	519:539	The optimum growth pH	519:539	The optimum growth pH and temperature for the isolate were pH 7.0-7.5 and 25 °C, respectively.
29932388	3	48	theme	sequence	304:311	arg1	analyses					313:320	gene sequence analyses	299:320	The 16S rRNA gene sequence analyses	286:320	The 16S rRNA gene sequence analyses showed that strain SJW1-2T was most closely related to Deinococcus metalli 1PNM-19T (94.3 % sequence similarity) and formed a robust phylogenetic clade with other species of the genus Deinococcus.
29932388	2	49	theme	freshwater	214:223	arg1	samples					225:231	freshwater samples	214:231	freshwater samples collected from the Seomjin River, Republic of Korea	214:283	A gamma radiation-resistant, Gram-stain-negative, rod-shaped bacterial strain, designated SJW1-2T, was isolated from freshwater samples collected from the Seomjin River, Republic of Korea.
29932388	4	50	theme	optimum	523:529	arg1	pH					578:579	pH 7.0-7.5	578:587	pH 7.0-7.5	578:587	The optimum growth pH and temperature for the isolate were pH 7.0-7.5 and 25 °C, respectively.
29932388	4	50	theme	optimum	523:529	arg1	pH					538:539	The optimum growth pH	519:539	The optimum growth pH	519:539	The optimum growth pH and temperature for the isolate were pH 7.0-7.5 and 25 °C, respectively.
29932388	3	51	theme	16S	290:292	arg1	rRNA					294:297	The 16S rRNA gene sequence analyses	286:320	The 16S rRNA gene sequence analyses	286:320	The 16S rRNA gene sequence analyses showed that strain SJW1-2T was most closely related to Deinococcus metalli 1PNM-19T (94.3 % sequence similarity) and formed a robust phylogenetic clade with other species of the genus Deinococcus.
29932388	10	52	theme	19332T=NBRC	1227:1237	arg1	SJW1-2T					1212:1218	strain SJW1-2T	1205:1218	strain SJW1-2T (=KACC 19332T=NBRC 112908T)	1205:1246	On the basis of phenotypic, genotypic and phylogenetic analyses, strain SJW1-2T (=KACC 19332T=NBRC 112908T) represents a novel species of the genus Deinococcus, for which the name Deinococcus koreensis sp.
29932388	10	52	theme	19332T=NBRC	1227:1237	arg1	112908T					1239:1245	=KACC 19332T=NBRC 112908T	1221:1245	=KACC 19332T=NBRC 112908T	1221:1245	On the basis of phenotypic, genotypic and phylogenetic analyses, strain SJW1-2T (=KACC 19332T=NBRC 112908T) represents a novel species of the genus Deinococcus, for which the name Deinococcus koreensis sp.
29932388	3	53	theme	Deinococcus	506:516	arg1	species					485:491	other species	479:491	other species of the genus Deinococcus	479:516	The 16S rRNA gene sequence analyses showed that strain SJW1-2T was most closely related to Deinococcus metalli 1PNM-19T (94.3 % sequence similarity) and formed a robust phylogenetic clade with other species of the genus Deinococcus.
29932388	8	54	theme	amino	913:917	arg1	alanine					930:936	alanine	930:936	alanine	930:936	The major peptidoglycan amino acids were alanine, d-glutamic acid, glycine and l-ornithine.
29932388	8	54	theme	amino	913:917	arg1	acids					919:923	The major peptidoglycan amino acids	889:923	The major peptidoglycan amino acids	889:923	The major peptidoglycan amino acids were alanine, d-glutamic acid, glycine and l-ornithine.
29932388	9	55	theme	fatty	997:1001	arg1	acids					1003:1007	The predominant fatty acids	981:1007	The predominant fatty acids (>10 %)	981:1015	The predominant fatty acids (>10 %) were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) (25.2 %) and C16 : 0 (21.2 %), and the DNA G+C content was 69.5 mol%.
29932388	9	55	theme	fatty	997:1001	arg1	%					1014:1014	>10 %	1010:1014	>10 %	1010:1014	The predominant fatty acids (>10 %) were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) (25.2 %) and C16 : 0 (21.2 %), and the DNA G+C content was 69.5 mol%.
29932388	3	56	theme	genus	500:504	arg1	Deinococcus					506:516	the genus Deinococcus	496:516	the genus Deinococcus	496:516	The 16S rRNA gene sequence analyses showed that strain SJW1-2T was most closely related to Deinococcus metalli 1PNM-19T (94.3 % sequence similarity) and formed a robust phylogenetic clade with other species of the genus Deinococcus.
29932388	7	57	theme	lipid	731:735	arg1	profile					737:743	The polar lipid profile	721:743	The polar lipid profile	721:743	The polar lipid profile consisted of different unidentified glycolipids, two unidentified lipids, two unidentified phospholipids and an unidentified phosphoglycolipid.
29932388	3	58	theme	strain	334:339	arg1	SJW1-2T					341:347	strain SJW1-2T	334:347	strain SJW1-2T	334:347	The 16S rRNA gene sequence analyses showed that strain SJW1-2T was most closely related to Deinococcus metalli 1PNM-19T (94.3 % sequence similarity) and formed a robust phylogenetic clade with other species of the genus Deinococcus.
29932388	1	59	theme	gamma	34:38	arg1	bacterium					60:68	a gamma radiation-resistant bacterium	32:68	a gamma radiation-resistant bacterium isolated from river water	32:94	nov., a gamma radiation-resistant bacterium isolated from river water.
29932388	1	59	theme	gamma	34:38	arg1	nov.					26:29	nov.	26:29	nov.	26:29	nov., a gamma radiation-resistant bacterium isolated from river water.
29932388	10	60	theme	novel	1261:1265	arg1	species					1267:1273	a novel species	1259:1273	a novel species	1259:1273	On the basis of phenotypic, genotypic and phylogenetic analyses, strain SJW1-2T (=KACC 19332T=NBRC 112908T) represents a novel species of the genus Deinococcus, for which the name Deinococcus koreensis sp.
29932388	0	61	theme	koreensis	12:20	arg1	sp					22:23	Deinococcus koreensis sp	0:23	Deinococcus koreensis sp.	0:24	Deinococcus koreensis sp.
29932388	10	62	theme	genotypic	1168:1176	arg1	analyses					1195:1202	phenotypic, genotypic and phylogenetic analyses	1156:1202	phenotypic, genotypic and phylogenetic analyses	1156:1202	On the basis of phenotypic, genotypic and phylogenetic analyses, strain SJW1-2T (=KACC 19332T=NBRC 112908T) represents a novel species of the genus Deinococcus, for which the name Deinococcus koreensis sp.
29608690	11	0	theme	16S	1605:1607	arg1	sequencing					1614:1623	The 16S rRNA sequencing	1601:1623	The 16S rRNA sequencing	1601:1623	The 16S rRNA sequencing revealed significant differences in the composition of WT and miR-21-/- intestinal microbiota.
29608690	9	1	from	miR-21-/-	1339:1347	arg1	homogenate					1323:1332	fecal homogenate	1317:1332	fecal homogenate from miR-21-/-	1317:1347	Co-housing conferred some protection to WT mice, while GF mice colonized with fecal homogenate from miR-21-/- were protected from DSS colitis compared with those colonized with WT homogenate.
29608690	9	2	theme	GF	1294:1295	arg1	mice					1297:1300	GF mice	1294:1300	GF mice colonized with fecal homogenate from miR-21-/-	1294:1347	Co-housing conferred some protection to WT mice, while GF mice colonized with fecal homogenate from miR-21-/- were protected from DSS colitis compared with those colonized with WT homogenate.
29608690	5	3	from	influence	606:614	arg1	microbiota					637:646	the gut microbiota	629:646	the gut microbiota	629:646	Thus, we determined the influence of miR-21 on the gut microbiota and observed the subsequent impact in a dextran sodium sulphate [DSS]-induced colitis model.
29608690	6	4	theme	antibiotic	892:901	arg1	depletion					903:911	antibiotic depletion	892:911	antibiotic depletion of the microbiota	892:929	METHODS The influence of miR-21 on the gut microbiota and inflammation was assessed in wild-type [WT] and miR-21-/- mice, in co-housed mice, following antibiotic depletion of the microbiota, or by colonization of germ-free [GF] mice with fecal homogenate, prior to DSS administration.
29608690	11	5	theme	miR-21-/-	1687:1695	arg1	microbiota					1708:1717	miR-21-/- intestinal microbiota	1687:1717	miR-21-/- intestinal microbiota	1687:1717	The 16S rRNA sequencing revealed significant differences in the composition of WT and miR-21-/- intestinal microbiota.
29608690	1	6	theme	gene	151:154	arg1	expression					156:165	gene expression	151:165	gene expression	151:165	BACKGROUND AND AIMS microRNAs regulate gene expression and influence the pathogenesis of human diseases.
29608690	6	7	theme	mice	969:972	arg1	colonization					938:949	colonization	938:949	colonization of germ-free [GF] mice with fecal homogenate	938:994	METHODS The influence of miR-21 on the gut microbiota and inflammation was assessed in wild-type [WT] and miR-21-/- mice, in co-housed mice, following antibiotic depletion of the microbiota, or by colonization of germ-free [GF] mice with fecal homogenate, prior to DSS administration.
29608690	0	8	theme	Murine	87:92	arg1	Model					94:98	a Murine Model	85:98	a Murine Model of Colitis	85:109	Loss of MicroRNA-21 Influences the Gut Microbiota, Causing Reduced Susceptibility in a Murine Model of Colitis.
29608690	9	9	theme	WT	1279:1280	arg1	mice					1282:1285	WT mice	1279:1285	WT mice	1279:1285	Co-housing conferred some protection to WT mice, while GF mice colonized with fecal homogenate from miR-21-/- were protected from DSS colitis compared with those colonized with WT homogenate.
29608690	5	10	theme	subsequent	665:674	arg1	impact					676:681	the subsequent impact	661:681	the subsequent impact	661:681	Thus, we determined the influence of miR-21 on the gut microbiota and observed the subsequent impact in a dextran sodium sulphate [DSS]-induced colitis model.
29608690	11	11	theme	rRNA	1609:1612	arg1	sequencing					1614:1623	The 16S rRNA sequencing	1601:1623	The 16S rRNA sequencing	1601:1623	The 16S rRNA sequencing revealed significant differences in the composition of WT and miR-21-/- intestinal microbiota.
29608690	1	12	dep	BACKGROUND	112:121	arg1	microRNAs					132:140	microRNAs	132:140	microRNAs	132:140	BACKGROUND AND AIMS microRNAs regulate gene expression and influence the pathogenesis of human diseases.
29608690	12	13	theme	intestinal	1798:1807	arg1	inflammation					1809:1820	intestinal inflammation	1798:1820	intestinal inflammation	1798:1820	CONCLUSIONS These findings suggest that miR-21 influences the pathogenesis of intestinal inflammation by causing propagation of a disrupted gut microbiota.
29608690	7	14	theme	miR-21-/-	1071:1079	arg1	mice					1081:1084	WT and miR-21-/- mice	1064:1084	WT and miR-21-/- mice	1064:1084	We carried out 16S rRNA sequencing on WT and miR-21-/- mice to dissect potential differences in the gut microbiota.
29608690	5	15	theme	miR-21	619:624	arg1	influence					606:614	the influence	602:614	the influence of miR-21 on the gut microbiota	602:646	Thus, we determined the influence of miR-21 on the gut microbiota and observed the subsequent impact in a dextran sodium sulphate [DSS]-induced colitis model.
29608690	6	16	theme	[GF	964:966	arg1	mice					969:972	germ-free [GF] mice	954:972	germ-free [GF] mice with fecal homogenate	954:994	METHODS The influence of miR-21 on the gut microbiota and inflammation was assessed in wild-type [WT] and miR-21-/- mice, in co-housed mice, following antibiotic depletion of the microbiota, or by colonization of germ-free [GF] mice with fecal homogenate, prior to DSS administration.
29608690	11	17	theme	significant	1634:1644	arg1	differences					1646:1656	significant differences	1634:1656	significant differences in the composition of WT and miR-21-/- intestinal microbiota	1634:1717	The 16S rRNA sequencing revealed significant differences in the composition of WT and miR-21-/- intestinal microbiota.
29608690	2	18	theme	[miR-21	272:278	arg1	role					252:255	the role	248:255	the role of microRNA-21 [miR-21] in the pathogenesis of intestinal inflammation	248:326	The present study investigated the role of microRNA-21 [miR-21] in the pathogenesis of intestinal inflammation, because miR-21 is highly expressed in inflammatory bowel disease.
29608690	8	19	theme	WT	1230:1231	arg1	mice					1233:1236	WT mice	1230:1236	WT mice	1230:1236	RESULTS miR-21-/- mice have reduced susceptibility to DSS-induced colitis compared with WT mice.
29608690	3	20	theme	intestinal	441:450	arg1	dysfunction					460:470	intestinal barrier dysfunction	441:470	intestinal barrier dysfunction	441:470	Inflammatory bowel disease is associated with intestinal barrier dysfunction and an altered gut microbiota.
29608690	7	21	theme	potential	1097:1105	arg1	differences					1107:1117	potential differences	1097:1117	potential differences in the gut microbiota	1097:1139	We carried out 16S rRNA sequencing on WT and miR-21-/- mice to dissect potential differences in the gut microbiota.
29608690	4	22	theme	Recent	503:508	arg1	studies					510:516	Recent studies	503:516	Recent studies	503:516	Recent studies have demonstrated that host microRNAs can shape the microbiota.
29608690	5	23	theme	gut	633:635	arg1	microbiota					637:646	the gut microbiota	629:646	the gut microbiota	629:646	Thus, we determined the influence of miR-21 on the gut microbiota and observed the subsequent impact in a dextran sodium sulphate [DSS]-induced colitis model.
29608690	2	24	theme	microRNA-21	260:270	arg1	[miR-21					272:278	microRNA-21 [miR-21]	260:279	microRNA-21 [miR-21]	260:279	The present study investigated the role of microRNA-21 [miR-21] in the pathogenesis of intestinal inflammation, because miR-21 is highly expressed in inflammatory bowel disease.
29608690	10	25	from	role	1452:1455	arg1	phenotype					1492:1500	the observed phenotype	1479:1500	the observed phenotype	1479:1500	Further supporting a role for the microbiota in the observed phenotype, the protection afforded by miR-21 depletion was lost when mice were pre-treated with antibiotics.
29608690	3	26	theme	barrier	452:458	arg1	dysfunction					460:470	intestinal barrier dysfunction	441:470	intestinal barrier dysfunction	441:470	Inflammatory bowel disease is associated with intestinal barrier dysfunction and an altered gut microbiota.
29608690	8	27	theme	miR-21-/-	1150:1158	arg1	mice					1160:1163	RESULTS miR-21-/- mice	1142:1163	RESULTS miR-21-/- mice	1142:1163	RESULTS miR-21-/- mice have reduced susceptibility to DSS-induced colitis compared with WT mice.
29608690	8	28	theme	RESULTS	1142:1148	arg1	mice					1160:1163	RESULTS miR-21-/- mice	1142:1163	RESULTS miR-21-/- mice	1142:1163	RESULTS miR-21-/- mice have reduced susceptibility to DSS-induced colitis compared with WT mice.
29608690	7	29	theme	rRNA	1045:1048	arg1	sequencing					1050:1059	rRNA sequencing	1045:1059	rRNA sequencing	1045:1059	We carried out 16S rRNA sequencing on WT and miR-21-/- mice to dissect potential differences in the gut microbiota.
29608690	6	30	theme	gut	780:782	arg1	microbiota					784:793	the gut microbiota and inflammation	776:810	microbiota	784:793	METHODS The influence of miR-21 on the gut microbiota and inflammation was assessed in wild-type [WT] and miR-21-/- mice, in co-housed mice, following antibiotic depletion of the microbiota, or by colonization of germ-free [GF] mice with fecal homogenate, prior to DSS administration.
29608690	2	31	theme	intestinal	304:313	arg1	inflammation					315:326	intestinal inflammation	304:326	intestinal inflammation	304:326	The present study investigated the role of microRNA-21 [miR-21] in the pathogenesis of intestinal inflammation, because miR-21 is highly expressed in inflammatory bowel disease.
29608690	2	32	theme	bowel	380:384	arg1	disease					386:392	inflammatory bowel disease	367:392	inflammatory bowel disease	367:392	The present study investigated the role of microRNA-21 [miR-21] in the pathogenesis of intestinal inflammation, because miR-21 is highly expressed in inflammatory bowel disease.
29608690	7	33	from	differences	1107:1117	arg1	microbiota					1130:1139	the gut microbiota	1122:1139	the gut microbiota	1122:1139	We carried out 16S rRNA sequencing on WT and miR-21-/- mice to dissect potential differences in the gut microbiota.
29608690	6	34	theme	germ-free	954:962	arg1	mice					969:972	germ-free [GF] mice	954:972	germ-free [GF] mice with fecal homogenate	954:994	METHODS The influence of miR-21 on the gut microbiota and inflammation was assessed in wild-type [WT] and miR-21-/- mice, in co-housed mice, following antibiotic depletion of the microbiota, or by colonization of germ-free [GF] mice with fecal homogenate, prior to DSS administration.
29608690	5	35	theme	colitis	726:732	arg1	model					734:738	a dextran sodium sulphate [DSS]-induced colitis model	686:738	a dextran sodium sulphate [DSS]-induced colitis model	686:738	Thus, we determined the influence of miR-21 on the gut microbiota and observed the subsequent impact in a dextran sodium sulphate [DSS]-induced colitis model.
29608690	2	36	theme	inflammatory	367:378	arg1	disease					386:392	inflammatory bowel disease	367:392	inflammatory bowel disease	367:392	The present study investigated the role of microRNA-21 [miR-21] in the pathogenesis of intestinal inflammation, because miR-21 is highly expressed in inflammatory bowel disease.
29608690	11	37	from	differences	1646:1656	arg1	composition					1665:1675	the composition	1661:1675	the composition of WT and miR-21-/- intestinal microbiota	1661:1717	The 16S rRNA sequencing revealed significant differences in the composition of WT and miR-21-/- intestinal microbiota.
29608690	0	38	theme	MicroRNA-21	8:18	arg1	Loss					0:3	Loss	0:3	Loss of MicroRNA-21	0:18	Loss of MicroRNA-21 Influences the Gut Microbiota, Causing Reduced Susceptibility in a Murine Model of Colitis.
29608690	0	39	theme	Colitis	103:109	arg1	Model					94:98	a Murine Model	85:98	a Murine Model of Colitis	85:109	Loss of MicroRNA-21 Influences the Gut Microbiota, Causing Reduced Susceptibility in a Murine Model of Colitis.
29608690	11	40	theme	WT	1680:1681	arg1	composition					1665:1675	the composition	1661:1675	the composition of WT and miR-21-/- intestinal microbiota	1661:1717	The 16S rRNA sequencing revealed significant differences in the composition of WT and miR-21-/- intestinal microbiota.
29608690	3	41	theme	altered	479:485	arg1	microbiota					491:500	an altered gut microbiota	476:500	an altered gut microbiota	476:500	Inflammatory bowel disease is associated with intestinal barrier dysfunction and an altered gut microbiota.
29608690	0	42	theme	Gut	35:37	arg1	Microbiota					39:48	the Gut Microbiota	31:48	the Gut Microbiota	31:48	Loss of MicroRNA-21 Influences the Gut Microbiota, Causing Reduced Susceptibility in a Murine Model of Colitis.
29608690	9	43	theme	fecal	1317:1321	arg1	homogenate					1323:1332	fecal homogenate	1317:1332	fecal homogenate from miR-21-/-	1317:1347	Co-housing conferred some protection to WT mice, while GF mice colonized with fecal homogenate from miR-21-/- were protected from DSS colitis compared with those colonized with WT homogenate.
29608690	6	44	theme	DSS	1006:1008	arg1	administration					1010:1023	DSS administration	1006:1023	DSS administration	1006:1023	METHODS The influence of miR-21 on the gut microbiota and inflammation was assessed in wild-type [WT] and miR-21-/- mice, in co-housed mice, following antibiotic depletion of the microbiota, or by colonization of germ-free [GF] mice with fecal homogenate, prior to DSS administration.
29608690	2	45	from	role	252:255	arg1	pathogenesis					288:299	the pathogenesis	284:299	the pathogenesis of intestinal inflammation	284:326	The present study investigated the role of microRNA-21 [miR-21] in the pathogenesis of intestinal inflammation, because miR-21 is highly expressed in inflammatory bowel disease.
29608690	1	46	theme	human	201:205	arg1	diseases					207:214	human diseases	201:214	human diseases	201:214	BACKGROUND AND AIMS microRNAs regulate gene expression and influence the pathogenesis of human diseases.
29608690	8	47	theme	reduced	1170:1176	arg1	susceptibility					1178:1191	reduced susceptibility	1170:1191	reduced susceptibility to DSS-induced colitis	1170:1214	RESULTS miR-21-/- mice have reduced susceptibility to DSS-induced colitis compared with WT mice.
29608690	5	48	theme	-induced	717:724	arg1	model					734:738	a dextran sodium sulphate [DSS]-induced colitis model	686:738	a dextran sodium sulphate [DSS]-induced colitis model	686:738	Thus, we determined the influence of miR-21 on the gut microbiota and observed the subsequent impact in a dextran sodium sulphate [DSS]-induced colitis model.
29608690	6	49	theme	miR-21-/-	847:855	arg1	mice					857:860	wild-type [WT] and miR-21-/- mice	828:860	mice	857:860	METHODS The influence of miR-21 on the gut microbiota and inflammation was assessed in wild-type [WT] and miR-21-/- mice, in co-housed mice, following antibiotic depletion of the microbiota, or by colonization of germ-free [GF] mice with fecal homogenate, prior to DSS administration.
29608690	2	50	theme	present	221:227	arg1	study					229:233	The present study	217:233	The present study	217:233	The present study investigated the role of microRNA-21 [miR-21] in the pathogenesis of intestinal inflammation, because miR-21 is highly expressed in inflammatory bowel disease.
29608690	6	51	theme	miR-21	766:771	arg1	influence					753:761	The influence	749:761	The influence of miR-21 on the gut microbiota and inflammation	749:810	METHODS The influence of miR-21 on the gut microbiota and inflammation was assessed in wild-type [WT] and miR-21-/- mice, in co-housed mice, following antibiotic depletion of the microbiota, or by colonization of germ-free [GF] mice with fecal homogenate, prior to DSS administration.
29608690	1	52	theme	diseases	207:214	arg1	pathogenesis					185:196	the pathogenesis	181:196	the pathogenesis of human diseases	181:214	BACKGROUND AND AIMS microRNAs regulate gene expression and influence the pathogenesis of human diseases.
29608690	3	53	theme	Inflammatory	395:406	arg1	disease					414:420	Inflammatory bowel disease	395:420	Inflammatory bowel disease	395:420	Inflammatory bowel disease is associated with intestinal barrier dysfunction and an altered gut microbiota.
29608690	4	54	theme	host	541:544	arg1	microRNAs					546:554	host microRNAs	541:554	host microRNAs	541:554	Recent studies have demonstrated that host microRNAs can shape the microbiota.
29608690	12	55	theme	disrupted	1850:1858	arg1	microbiota					1864:1873	a disrupted gut microbiota	1848:1873	a disrupted gut microbiota	1848:1873	CONCLUSIONS These findings suggest that miR-21 influences the pathogenesis of intestinal inflammation by causing propagation of a disrupted gut microbiota.
29608690	6	56	theme	co-housed	866:874	arg1	mice					876:879	co-housed mice	866:879	co-housed mice	866:879	METHODS The influence of miR-21 on the gut microbiota and inflammation was assessed in wild-type [WT] and miR-21-/- mice, in co-housed mice, following antibiotic depletion of the microbiota, or by colonization of germ-free [GF] mice with fecal homogenate, prior to DSS administration.
29608690	6	57	from	influence	753:761	arg1	inflammation					799:810	the gut microbiota and inflammation	776:810	inflammation	799:810	METHODS The influence of miR-21 on the gut microbiota and inflammation was assessed in wild-type [WT] and miR-21-/- mice, in co-housed mice, following antibiotic depletion of the microbiota, or by colonization of germ-free [GF] mice with fecal homogenate, prior to DSS administration.
29608690	6	57	from	influence	753:761	arg1	microbiota					784:793	the gut microbiota and inflammation	776:810	microbiota	784:793	METHODS The influence of miR-21 on the gut microbiota and inflammation was assessed in wild-type [WT] and miR-21-/- mice, in co-housed mice, following antibiotic depletion of the microbiota, or by colonization of germ-free [GF] mice with fecal homogenate, prior to DSS administration.
29608690	3	58	theme	bowel	408:412	arg1	disease					414:420	Inflammatory bowel disease	395:420	Inflammatory bowel disease	395:420	Inflammatory bowel disease is associated with intestinal barrier dysfunction and an altered gut microbiota.
29608690	6	59	theme	microbiota	920:929	arg1	depletion					903:911	antibiotic depletion	892:911	antibiotic depletion of the microbiota	892:929	METHODS The influence of miR-21 on the gut microbiota and inflammation was assessed in wild-type [WT] and miR-21-/- mice, in co-housed mice, following antibiotic depletion of the microbiota, or by colonization of germ-free [GF] mice with fecal homogenate, prior to DSS administration.
29608690	11	60	theme	microbiota	1708:1717	arg1	composition					1665:1675	the composition	1661:1675	the composition of WT and miR-21-/- intestinal microbiota	1661:1717	The 16S rRNA sequencing revealed significant differences in the composition of WT and miR-21-/- intestinal microbiota.
29608690	7	61	theme	WT	1064:1065	arg1	mice					1081:1084	WT and miR-21-/- mice	1064:1084	WT and miR-21-/- mice	1064:1084	We carried out 16S rRNA sequencing on WT and miR-21-/- mice to dissect potential differences in the gut microbiota.
29608690	10	62	theme	miR-21	1530:1535	arg1	depletion					1537:1545	miR-21 depletion	1530:1545	miR-21 depletion	1530:1545	Further supporting a role for the microbiota in the observed phenotype, the protection afforded by miR-21 depletion was lost when mice were pre-treated with antibiotics.
29608690	10	63	theme	observed	1483:1490	arg1	phenotype					1492:1500	the observed phenotype	1479:1500	the observed phenotype	1479:1500	Further supporting a role for the microbiota in the observed phenotype, the protection afforded by miR-21 depletion was lost when mice were pre-treated with antibiotics.
29608690	12	64	theme	microbiota	1864:1873	arg1	propagation					1833:1843	propagation	1833:1843	propagation of a disrupted gut microbiota	1833:1873	CONCLUSIONS These findings suggest that miR-21 influences the pathogenesis of intestinal inflammation by causing propagation of a disrupted gut microbiota.
29608690	3	65	theme	gut	487:489	arg1	microbiota					491:500	an altered gut microbiota	476:500	an altered gut microbiota	476:500	Inflammatory bowel disease is associated with intestinal barrier dysfunction and an altered gut microbiota.
29608690	12	66	dep	CONCLUSIONS	1720:1730	arg1	suggest					1747:1753	suggest	1747:1753	suggest that miR-21 influences the pathogenesis of intestinal inflammation by causing propagation of a disrupted gut microbiota	1747:1873	CONCLUSIONS These findings suggest that miR-21 influences the pathogenesis of intestinal inflammation by causing propagation of a disrupted gut microbiota.
29608690	12	67	theme	gut	1860:1862	arg1	microbiota					1864:1873	a disrupted gut microbiota	1848:1873	a disrupted gut microbiota	1848:1873	CONCLUSIONS These findings suggest that miR-21 influences the pathogenesis of intestinal inflammation by causing propagation of a disrupted gut microbiota.
29608690	6	68	theme	wild-type	828:836	arg1	[WT					838:840	wild-type [WT] and miR-21-/- mice	828:860	[WT	838:840	METHODS The influence of miR-21 on the gut microbiota and inflammation was assessed in wild-type [WT] and miR-21-/- mice, in co-housed mice, following antibiotic depletion of the microbiota, or by colonization of germ-free [GF] mice with fecal homogenate, prior to DSS administration.
29608690	8	69	contain	have	1165:1168	arg1	mice					1160:1163	RESULTS miR-21-/- mice	1142:1163	RESULTS miR-21-/- mice	1142:1163	RESULTS miR-21-/- mice have reduced susceptibility to DSS-induced colitis compared with WT mice.
29608690	8	69	contain	have	1165:1168	arg2	susceptibility					1178:1191	reduced susceptibility	1170:1191	reduced susceptibility to DSS-induced colitis	1170:1214	RESULTS miR-21-/- mice have reduced susceptibility to DSS-induced colitis compared with WT mice.
29608690	2	70	theme	inflammation	315:326	arg1	pathogenesis					288:299	the pathogenesis	284:299	the pathogenesis of intestinal inflammation	284:326	The present study investigated the role of microRNA-21 [miR-21] in the pathogenesis of intestinal inflammation, because miR-21 is highly expressed in inflammatory bowel disease.
29608690	6	71	dep	METHODS	741:747	arg1	assessed					816:823	assessed	816:823	assessed	816:823	METHODS The influence of miR-21 on the gut microbiota and inflammation was assessed in wild-type [WT] and miR-21-/- mice, in co-housed mice, following antibiotic depletion of the microbiota, or by colonization of germ-free [GF] mice with fecal homogenate, prior to DSS administration.
29608690	0	72	theme	Reduced	59:65	arg1	Susceptibility					67:80	Reduced Susceptibility	59:80	Reduced Susceptibility	59:80	Loss of MicroRNA-21 Influences the Gut Microbiota, Causing Reduced Susceptibility in a Murine Model of Colitis.
29608690	11	73	theme	intestinal	1697:1706	arg1	microbiota					1708:1717	miR-21-/- intestinal microbiota	1687:1717	miR-21-/- intestinal microbiota	1687:1717	The 16S rRNA sequencing revealed significant differences in the composition of WT and miR-21-/- intestinal microbiota.
29608690	9	74	theme	WT	1416:1417	arg1	homogenate					1419:1428	WT homogenate	1416:1428	WT homogenate	1416:1428	Co-housing conferred some protection to WT mice, while GF mice colonized with fecal homogenate from miR-21-/- were protected from DSS colitis compared with those colonized with WT homogenate.
29608690	9	75	theme	DSS	1369:1371	arg1	colitis					1373:1379	DSS colitis	1369:1379	DSS colitis	1369:1379	Co-housing conferred some protection to WT mice, while GF mice colonized with fecal homogenate from miR-21-/- were protected from DSS colitis compared with those colonized with WT homogenate.
29608690	8	76	theme	DSS-induced	1196:1206	arg1	colitis					1208:1214	DSS-induced colitis	1196:1214	DSS-induced colitis	1196:1214	RESULTS miR-21-/- mice have reduced susceptibility to DSS-induced colitis compared with WT mice.
29608690	6	77	with	mice	969:972	arg1	homogenate					985:994	fecal homogenate	979:994	fecal homogenate	979:994	METHODS The influence of miR-21 on the gut microbiota and inflammation was assessed in wild-type [WT] and miR-21-/- mice, in co-housed mice, following antibiotic depletion of the microbiota, or by colonization of germ-free [GF] mice with fecal homogenate, prior to DSS administration.
29608690	12	78	theme	inflammation	1809:1820	arg1	pathogenesis					1782:1793	the pathogenesis	1778:1793	the pathogenesis of intestinal inflammation	1778:1820	CONCLUSIONS These findings suggest that miR-21 influences the pathogenesis of intestinal inflammation by causing propagation of a disrupted gut microbiota.
29608690	7	79	theme	gut	1126:1128	arg1	microbiota					1130:1139	the gut microbiota	1122:1139	the gut microbiota	1122:1139	We carried out 16S rRNA sequencing on WT and miR-21-/- mice to dissect potential differences in the gut microbiota.
29608690	6	80	theme	fecal	979:983	arg1	homogenate					985:994	fecal homogenate	979:994	fecal homogenate	979:994	METHODS The influence of miR-21 on the gut microbiota and inflammation was assessed in wild-type [WT] and miR-21-/- mice, in co-housed mice, following antibiotic depletion of the microbiota, or by colonization of germ-free [GF] mice with fecal homogenate, prior to DSS administration.
29465337	7	0	theme	Strain	956:961	arg1	N237T					963:967	Strain N237T	956:967	Strain N237T	956:967	Strain N237T formed a separate lineage in the genus Jatrophihabitans, as demonstrated by phylogenetic analysis based on 16S rRNA sequencing.
29465337	4	1	theme	major	520:524	arg1	acids					532:536	the major fatty acids	516:536	the major fatty acids	516:536	Strain N237T contained iso-C16 : 0 and C17 : 1ω8c as the major fatty acids, MK-9(H4) as the predominant isoprenoid quinone and meso-DAP as the diamino acid in the peptidoglycan.
29465337	4	2	theme	diamino	606:612	arg1	iso-C16 					486:493	iso-C16 	486:493	iso-C16 	486:493	Strain N237T contained iso-C16 : 0 and C17 : 1ω8c as the major fatty acids, MK-9(H4) as the predominant isoprenoid quinone and meso-DAP as the diamino acid in the peptidoglycan.
29465337	4	2	theme	diamino	606:612	arg1	acid					614:617	the diamino acid	602:617	the diamino acid in the peptidoglycan	602:638	Strain N237T contained iso-C16 : 0 and C17 : 1ω8c as the major fatty acids, MK-9(H4) as the predominant isoprenoid quinone and meso-DAP as the diamino acid in the peptidoglycan.
29465337	8	3	theme	Jatrophihabitans	1128:1143	arg1	similarity					1177:1186	95.6 % sequence similarity	1161:1186	95.6 % sequence similarity	1161:1186	It was most closely related to Jatrophihabitans soli KIS75-12T (95.6 % sequence similarity).
29465337	8	3	theme	Jatrophihabitans	1128:1143	arg1	KIS75-12T					1150:1158	Jatrophihabitans soli KIS75-12T	1128:1158	Jatrophihabitans soli KIS75-12T (95.6 % sequence similarity)	1128:1187	It was most closely related to Jatrophihabitans soli KIS75-12T (95.6 % sequence similarity).
29465337	4	4	dep	iso-C16 	486:493	arg1	 0					495:496	 0	495:496	 0	495:496	Strain N237T contained iso-C16 : 0 and C17 : 1ω8c as the major fatty acids, MK-9(H4) as the predominant isoprenoid quinone and meso-DAP as the diamino acid in the peptidoglycan.
29465337	4	4	dep	iso-C16 	486:493	arg1	 1ω8c					507:511	 1ω8c	507:511	 1ω8c as the major fatty acids	507:536	Strain N237T contained iso-C16 : 0 and C17 : 1ω8c as the major fatty acids, MK-9(H4) as the predominant isoprenoid quinone and meso-DAP as the diamino acid in the peptidoglycan.
29465337	4	4	dep	iso-C16 	486:493	arg1	C17 					502:505	C17 	502:505	C17 	502:505	Strain N237T contained iso-C16 : 0 and C17 : 1ω8c as the major fatty acids, MK-9(H4) as the predominant isoprenoid quinone and meso-DAP as the diamino acid in the peptidoglycan.
29465337	7	5	theme	rRNA	1080:1083	arg1	sequencing					1085:1094	16S rRNA sequencing	1076:1094	16S rRNA sequencing	1076:1094	Strain N237T formed a separate lineage in the genus Jatrophihabitans, as demonstrated by phylogenetic analysis based on 16S rRNA sequencing.
29465337	1	6	theme	sediment	50:57	arg1	soil					59:62	sediment soil	50:62	sediment soil of lava forest wetlands	50:86	nov., isolated from sediment soil of lava forest wetlands and the emended description of the genus Jatrophihabitans.
29465337	2	7	attach	isolated	201:208	arg1	soil					224:227	sediment soil	215:227	sediment soil of wetlands at Meonmulkkak, Dongbaek-Dongsan, the lava forest, Gotjawal, Jeju, Republic of Korea	215:324	A novel actinobacterial strain, designated N237T, was isolated from sediment soil of wetlands at Meonmulkkak, Dongbaek-Dongsan, the lava forest, Gotjawal, Jeju, Republic of Korea.
29465337	2	7	attach	isolated	201:208	arg2	strain					171:176	A novel actinobacterial strain	147:176	A novel actinobacterial strain	147:176	A novel actinobacterial strain, designated N237T, was isolated from sediment soil of wetlands at Meonmulkkak, Dongbaek-Dongsan, the lava forest, Gotjawal, Jeju, Republic of Korea.
29465337	4	8	theme	fatty	526:530	arg1	acids					532:536	the major fatty acids	516:536	the major fatty acids	516:536	Strain N237T contained iso-C16 : 0 and C17 : 1ω8c as the major fatty acids, MK-9(H4) as the predominant isoprenoid quinone and meso-DAP as the diamino acid in the peptidoglycan.
29465337	1	9	theme	emended	96:102	arg1	description					104:114	the emended description	92:114	the emended description of the genus Jatrophihabitans	92:144	nov., isolated from sediment soil of lava forest wetlands and the emended description of the genus Jatrophihabitans.
29465337	3	10	dep	Gram-stain-positive	354:372	arg1	non-motile					375:384	non-motile	375:384	non-motile	375:384	Cells of strain N237T were Gram-stain-positive, non-motile rods and formed pale yellow colonies on ten-fold diluted Reasoner's 2A agar.
29465337	11	11	theme	type	1435:1438	arg1	N237T					1450:1454	N237T	1450:1454	N237T (=KCTC 39922T=NRRL B-65477T)	1450:1483	The type strain is N237T (=KCTC 39922T=NRRL B-65477T).
29465337	11	11	theme	type	1435:1438	arg1	strain					1440:1445	The type strain	1431:1445	The type strain	1431:1445	The type strain is N237T (=KCTC 39922T=NRRL B-65477T).
29465337	3	12	theme	pale	402:405	arg1	colonies					414:421	pale yellow colonies	402:421	pale yellow colonies on ten-fold diluted Reasoner's 2A agar	402:460	Cells of strain N237T were Gram-stain-positive, non-motile rods and formed pale yellow colonies on ten-fold diluted Reasoner's 2A agar.
29465337	8	13	theme	soli	1145:1148	arg1	similarity					1177:1186	95.6 % sequence similarity	1161:1186	95.6 % sequence similarity	1161:1186	It was most closely related to Jatrophihabitans soli KIS75-12T (95.6 % sequence similarity).
29465337	8	13	theme	soli	1145:1148	arg1	KIS75-12T					1150:1158	Jatrophihabitans soli KIS75-12T	1128:1158	Jatrophihabitans soli KIS75-12T (95.6 % sequence similarity)	1128:1187	It was most closely related to Jatrophihabitans soli KIS75-12T (95.6 % sequence similarity).
29465337	5	14	contain	contained	644:652	arg2	glycophospholipids					825:842	two unidentified glycophospholipids	808:842	two unidentified glycophospholipids	808:842	It contained diphosphatidylglycerol, phosphatidylinositol polymannosides, an unidentified phospholipid, an unidentified aminophospholipid, an unidentified aminolipid, two unidentified glycophospholipids, three unidentified glycolipids and two unidentified lipids as polar lipids.
29465337	5	14	contain	contained	644:652	arg2	lipids					897:902	two unidentified lipids	880:902	two unidentified lipids as polar lipids	880:918	It contained diphosphatidylglycerol, phosphatidylinositol polymannosides, an unidentified phospholipid, an unidentified aminophospholipid, an unidentified aminolipid, two unidentified glycophospholipids, three unidentified glycolipids and two unidentified lipids as polar lipids.
29465337	5	14	contain	contained	644:652	arg2	polymannosides					699:712	phosphatidylinositol polymannosides	678:712	phosphatidylinositol polymannosides	678:712	It contained diphosphatidylglycerol, phosphatidylinositol polymannosides, an unidentified phospholipid, an unidentified aminophospholipid, an unidentified aminolipid, two unidentified glycophospholipids, three unidentified glycolipids and two unidentified lipids as polar lipids.
29465337	5	14	contain	contained	644:652	arg2	phospholipid					731:742	an unidentified phospholipid	715:742	an unidentified phospholipid	715:742	It contained diphosphatidylglycerol, phosphatidylinositol polymannosides, an unidentified phospholipid, an unidentified aminophospholipid, an unidentified aminolipid, two unidentified glycophospholipids, three unidentified glycolipids and two unidentified lipids as polar lipids.
29465337	5	14	contain	contained	644:652	arg2	diphosphatidylglycerol					654:675	diphosphatidylglycerol	654:675	diphosphatidylglycerol	654:675	It contained diphosphatidylglycerol, phosphatidylinositol polymannosides, an unidentified phospholipid, an unidentified aminophospholipid, an unidentified aminolipid, two unidentified glycophospholipids, three unidentified glycolipids and two unidentified lipids as polar lipids.
29465337	5	14	contain	contained	644:652	arg1	It					641:642	It	641:642	It	641:642	It contained diphosphatidylglycerol, phosphatidylinositol polymannosides, an unidentified phospholipid, an unidentified aminophospholipid, an unidentified aminolipid, two unidentified glycophospholipids, three unidentified glycolipids and two unidentified lipids as polar lipids.
29465337	5	14	contain	contained	644:652	arg2	aminolipid					796:805	an unidentified aminolipid	780:805	an unidentified aminolipid	780:805	It contained diphosphatidylglycerol, phosphatidylinositol polymannosides, an unidentified phospholipid, an unidentified aminophospholipid, an unidentified aminolipid, two unidentified glycophospholipids, three unidentified glycolipids and two unidentified lipids as polar lipids.
29465337	5	14	contain	contained	644:652	arg2	aminophospholipid					761:777	aminophospholipid	761:777	aminophospholipid	761:777	It contained diphosphatidylglycerol, phosphatidylinositol polymannosides, an unidentified phospholipid, an unidentified aminophospholipid, an unidentified aminolipid, two unidentified glycophospholipids, three unidentified glycolipids and two unidentified lipids as polar lipids.
29465337	5	14	contain	contained	644:652	arg2	glycolipids					864:874	three unidentified glycolipids	845:874	three unidentified glycolipids	845:874	It contained diphosphatidylglycerol, phosphatidylinositol polymannosides, an unidentified phospholipid, an unidentified aminophospholipid, an unidentified aminolipid, two unidentified glycophospholipids, three unidentified glycolipids and two unidentified lipids as polar lipids.
29465337	7	15	theme	16S	1076:1078	arg1	sequencing					1085:1094	16S rRNA sequencing	1076:1094	16S rRNA sequencing	1076:1094	Strain N237T formed a separate lineage in the genus Jatrophihabitans, as demonstrated by phylogenetic analysis based on 16S rRNA sequencing.
29465337	1	16	theme	lava	67:70	arg1	wetlands					79:86	lava forest wetlands	67:86	lava forest wetlands	67:86	nov., isolated from sediment soil of lava forest wetlands and the emended description of the genus Jatrophihabitans.
29465337	2	17	from	soil	224:227	arg1	Republic					308:315	Republic	308:315	Republic	308:315	A novel actinobacterial strain, designated N237T, was isolated from sediment soil of wetlands at Meonmulkkak, Dongbaek-Dongsan, the lava forest, Gotjawal, Jeju, Republic of Korea.
29465337	2	17	from	soil	224:227	arg1	Dongbaek-Dongsan					257:272	Dongbaek-Dongsan	257:272	Dongbaek-Dongsan	257:272	A novel actinobacterial strain, designated N237T, was isolated from sediment soil of wetlands at Meonmulkkak, Dongbaek-Dongsan, the lava forest, Gotjawal, Jeju, Republic of Korea.
29465337	2	17	from	soil	224:227	arg1	Gotjawal					292:299	Gotjawal	292:299	Gotjawal	292:299	A novel actinobacterial strain, designated N237T, was isolated from sediment soil of wetlands at Meonmulkkak, Dongbaek-Dongsan, the lava forest, Gotjawal, Jeju, Republic of Korea.
29465337	3	18	theme	yellow	407:412	arg1	colonies					414:421	pale yellow colonies	402:421	pale yellow colonies on ten-fold diluted Reasoner's 2A agar	402:460	Cells of strain N237T were Gram-stain-positive, non-motile rods and formed pale yellow colonies on ten-fold diluted Reasoner's 2A agar.
29465337	3	19	theme	N237T	343:347	arg1	Cells					327:331	Cells	327:331	Cells of strain N237T	327:347	Cells of strain N237T were Gram-stain-positive, non-motile rods and formed pale yellow colonies on ten-fold diluted Reasoner's 2A agar.
29465337	9	20	theme	novel	1319:1323	arg1	species					1325:1331	a novel species	1317:1331	a novel species	1317:1331	The combined phenotypic, chemotaxonomic and phylogenetic characteristics supported the conclusion that strain N237T represents a novel species in the genus Jatrophihabitans, for which the name Jatrophihabitans telluris sp.
29465337	9	21	from	species	1325:1331	arg1	Jatrophihabitans					1346:1361	the genus Jatrophihabitans	1336:1361	the genus Jatrophihabitans	1336:1361	The combined phenotypic, chemotaxonomic and phylogenetic characteristics supported the conclusion that strain N237T represents a novel species in the genus Jatrophihabitans, for which the name Jatrophihabitans telluris sp.
29465337	9	22	theme	strain	1293:1298	arg1	N237T					1300:1304	strain N237T	1293:1304	strain N237T	1293:1304	The combined phenotypic, chemotaxonomic and phylogenetic characteristics supported the conclusion that strain N237T represents a novel species in the genus Jatrophihabitans, for which the name Jatrophihabitans telluris sp.
29465337	1	23	theme	forest	72:77	arg1	wetlands					79:86	lava forest wetlands	67:86	lava forest wetlands	67:86	nov., isolated from sediment soil of lava forest wetlands and the emended description of the genus Jatrophihabitans.
29465337	1	24	theme	wetlands	79:86	arg1	soil					59:62	sediment soil	50:62	sediment soil of lava forest wetlands	50:86	nov., isolated from sediment soil of lava forest wetlands and the emended description of the genus Jatrophihabitans.
29465337	1	24	theme	wetlands	79:86	arg1	description					104:114	the emended description	92:114	the emended description of the genus Jatrophihabitans	92:144	nov., isolated from sediment soil of lava forest wetlands and the emended description of the genus Jatrophihabitans.
29465337	9	25	theme	phenotypic	1203:1212	arg1	characteristics					1247:1261	The combined phenotypic, chemotaxonomic and phylogenetic characteristics	1190:1261	The combined phenotypic, chemotaxonomic and phylogenetic characteristics	1190:1261	The combined phenotypic, chemotaxonomic and phylogenetic characteristics supported the conclusion that strain N237T represents a novel species in the genus Jatrophihabitans, for which the name Jatrophihabitans telluris sp.
29465337	8	26	theme	sequence	1168:1175	arg1	similarity					1177:1186	95.6 % sequence similarity	1161:1186	95.6 % sequence similarity	1161:1186	It was most closely related to Jatrophihabitans soli KIS75-12T (95.6 % sequence similarity).
29465337	8	26	theme	sequence	1168:1175	arg1	KIS75-12T					1150:1158	Jatrophihabitans soli KIS75-12T	1128:1158	Jatrophihabitans soli KIS75-12T (95.6 % sequence similarity)	1128:1187	It was most closely related to Jatrophihabitans soli KIS75-12T (95.6 % sequence similarity).
29465337	6	27	theme	68.1 mol	945:952	arg1	G+C content					929:939	The DNA G+C content	921:939	The DNA G+C content	921:939	The DNA G+C content was 68.1 mol%.
29465337	6	27	theme	68.1 mol	945:952	arg1	%					953:953	68.1 mol%	945:953	68.1 mol%	945:953	The DNA G+C content was 68.1 mol%.
29465337	5	28	theme	polar	907:911	arg1	lipids					913:918	polar lipids	907:918	polar lipids	907:918	It contained diphosphatidylglycerol, phosphatidylinositol polymannosides, an unidentified phospholipid, an unidentified aminophospholipid, an unidentified aminolipid, two unidentified glycophospholipids, three unidentified glycolipids and two unidentified lipids as polar lipids.
29465337	5	29	theme	unidentified	783:794	arg1	aminolipid					796:805	an unidentified aminolipid	780:805	an unidentified aminolipid	780:805	It contained diphosphatidylglycerol, phosphatidylinositol polymannosides, an unidentified phospholipid, an unidentified aminophospholipid, an unidentified aminolipid, two unidentified glycophospholipids, three unidentified glycolipids and two unidentified lipids as polar lipids.
29465337	3	30	theme	strain	336:341	arg1	N237T					343:347	strain N237T	336:347	strain N237T	336:347	Cells of strain N237T were Gram-stain-positive, non-motile rods and formed pale yellow colonies on ten-fold diluted Reasoner's 2A agar.
29465337	7	31	theme	phylogenetic	1045:1056	arg1	analysis					1058:1065	phylogenetic analysis	1045:1065	phylogenetic analysis based on 16S rRNA sequencing	1045:1094	Strain N237T formed a separate lineage in the genus Jatrophihabitans, as demonstrated by phylogenetic analysis based on 16S rRNA sequencing.
29465337	9	32	theme	combined	1194:1201	arg1	characteristics					1247:1261	The combined phenotypic, chemotaxonomic and phylogenetic characteristics	1190:1261	The combined phenotypic, chemotaxonomic and phylogenetic characteristics	1190:1261	The combined phenotypic, chemotaxonomic and phylogenetic characteristics supported the conclusion that strain N237T represents a novel species in the genus Jatrophihabitans, for which the name Jatrophihabitans telluris sp.
29465337	5	33	theme	unidentified	851:862	arg1	glycophospholipids					825:842	two unidentified glycophospholipids	808:842	two unidentified glycophospholipids	808:842	It contained diphosphatidylglycerol, phosphatidylinositol polymannosides, an unidentified phospholipid, an unidentified aminophospholipid, an unidentified aminolipid, two unidentified glycophospholipids, three unidentified glycolipids and two unidentified lipids as polar lipids.
29465337	5	33	theme	unidentified	851:862	arg1	glycolipids					864:874	three unidentified glycolipids	845:874	three unidentified glycolipids	845:874	It contained diphosphatidylglycerol, phosphatidylinositol polymannosides, an unidentified phospholipid, an unidentified aminophospholipid, an unidentified aminolipid, two unidentified glycophospholipids, three unidentified glycolipids and two unidentified lipids as polar lipids.
29465337	5	34	theme	unidentified	718:729	arg1	phospholipid					731:742	an unidentified phospholipid	715:742	an unidentified phospholipid	715:742	It contained diphosphatidylglycerol, phosphatidylinositol polymannosides, an unidentified phospholipid, an unidentified aminophospholipid, an unidentified aminolipid, two unidentified glycophospholipids, three unidentified glycolipids and two unidentified lipids as polar lipids.
29465337	9	35	theme	genus	1340:1344	arg1	Jatrophihabitans					1346:1361	the genus Jatrophihabitans	1336:1361	the genus Jatrophihabitans	1336:1361	The combined phenotypic, chemotaxonomic and phylogenetic characteristics supported the conclusion that strain N237T represents a novel species in the genus Jatrophihabitans, for which the name Jatrophihabitans telluris sp.
29465337	7	36	from	lineage	987:993	arg1	Jatrophihabitans					1008:1023	the genus Jatrophihabitans	998:1023	the genus Jatrophihabitans	998:1023	Strain N237T formed a separate lineage in the genus Jatrophihabitans, as demonstrated by phylogenetic analysis based on 16S rRNA sequencing.
29465337	11	37	theme	39922T=NRRL	1463:1473	arg1	B-65477T					1475:1482	=KCTC 39922T=NRRL B-65477T	1457:1482	=KCTC 39922T=NRRL B-65477T	1457:1482	The type strain is N237T (=KCTC 39922T=NRRL B-65477T).
29465337	11	37	theme	39922T=NRRL	1463:1473	arg1	N237T					1450:1454	N237T	1450:1454	N237T (=KCTC 39922T=NRRL B-65477T)	1450:1483	The type strain is N237T (=KCTC 39922T=NRRL B-65477T).
29465337	4	38	theme	predominant	555:565	arg1	quinone					578:584	the predominant isoprenoid quinone	551:584	the predominant isoprenoid quinone	551:584	Strain N237T contained iso-C16 : 0 and C17 : 1ω8c as the major fatty acids, MK-9(H4) as the predominant isoprenoid quinone and meso-DAP as the diamino acid in the peptidoglycan.
29465337	4	38	theme	predominant	555:565	arg1	iso-C16 					486:493	iso-C16 	486:493	iso-C16 	486:493	Strain N237T contained iso-C16 : 0 and C17 : 1ω8c as the major fatty acids, MK-9(H4) as the predominant isoprenoid quinone and meso-DAP as the diamino acid in the peptidoglycan.
29465337	5	39	theme	unidentified	812:823	arg1	glycophospholipids					825:842	two unidentified glycophospholipids	808:842	two unidentified glycophospholipids	808:842	It contained diphosphatidylglycerol, phosphatidylinositol polymannosides, an unidentified phospholipid, an unidentified aminophospholipid, an unidentified aminolipid, two unidentified glycophospholipids, three unidentified glycolipids and two unidentified lipids as polar lipids.
29465337	5	39	theme	unidentified	812:823	arg1	glycolipids					864:874	three unidentified glycolipids	845:874	three unidentified glycolipids	845:874	It contained diphosphatidylglycerol, phosphatidylinositol polymannosides, an unidentified phospholipid, an unidentified aminophospholipid, an unidentified aminolipid, two unidentified glycophospholipids, three unidentified glycolipids and two unidentified lipids as polar lipids.
29465337	6	40	theme	DNA	925:927	arg1	G+C content					929:939	The DNA G+C content	921:939	The DNA G+C content	921:939	The DNA G+C content was 68.1 mol%.
29465337	6	40	theme	DNA	925:927	arg1	%					953:953	68.1 mol%	945:953	68.1 mol%	945:953	The DNA G+C content was 68.1 mol%.
29465337	2	41	theme	actinobacterial	155:169	arg1	strain					171:176	A novel actinobacterial strain	147:176	A novel actinobacterial strain	147:176	A novel actinobacterial strain, designated N237T, was isolated from sediment soil of wetlands at Meonmulkkak, Dongbaek-Dongsan, the lava forest, Gotjawal, Jeju, Republic of Korea.
29465337	2	42	theme	sediment	215:222	arg1	soil					224:227	sediment soil	215:227	sediment soil of wetlands at Meonmulkkak, Dongbaek-Dongsan, the lava forest, Gotjawal, Jeju, Republic of Korea	215:324	A novel actinobacterial strain, designated N237T, was isolated from sediment soil of wetlands at Meonmulkkak, Dongbaek-Dongsan, the lava forest, Gotjawal, Jeju, Republic of Korea.
29465337	3	43	from	colonies	414:421	arg1	agar					457:460	ten-fold diluted Reasoner's 2A agar	426:460	ten-fold diluted Reasoner's 2A agar	426:460	Cells of strain N237T were Gram-stain-positive, non-motile rods and formed pale yellow colonies on ten-fold diluted Reasoner's 2A agar.
29465337	8	44	theme	95.6 	1161:1165	arg1	%					1166:1166	%	1166:1166	%	1166:1166	It was most closely related to Jatrophihabitans soli KIS75-12T (95.6 % sequence similarity).
29465337	3	45	theme	Gram-stain-positive	354:372	arg1	rods					386:389	Gram-stain-positive, non-motile rods	354:389	Gram-stain-positive, non-motile rods	354:389	Cells of strain N237T were Gram-stain-positive, non-motile rods and formed pale yellow colonies on ten-fold diluted Reasoner's 2A agar.
29465337	2	46	theme	novel	149:153	arg1	strain					171:176	A novel actinobacterial strain	147:176	A novel actinobacterial strain	147:176	A novel actinobacterial strain, designated N237T, was isolated from sediment soil of wetlands at Meonmulkkak, Dongbaek-Dongsan, the lava forest, Gotjawal, Jeju, Republic of Korea.
29465337	3	47	theme	2A	454:455	arg1	agar					457:460	ten-fold diluted Reasoner's 2A agar	426:460	ten-fold diluted Reasoner's 2A agar	426:460	Cells of strain N237T were Gram-stain-positive, non-motile rods and formed pale yellow colonies on ten-fold diluted Reasoner's 2A agar.
29465337	4	48	contain	contained	476:484	arg2	iso-C16 					486:493	iso-C16 	486:493	iso-C16 	486:493	Strain N237T contained iso-C16 : 0 and C17 : 1ω8c as the major fatty acids, MK-9(H4) as the predominant isoprenoid quinone and meso-DAP as the diamino acid in the peptidoglycan.
29465337	4	48	contain	contained	476:484	arg2	MK-9					539:542	MK-9	539:542	MK-9(H4)	539:546	Strain N237T contained iso-C16 : 0 and C17 : 1ω8c as the major fatty acids, MK-9(H4) as the predominant isoprenoid quinone and meso-DAP as the diamino acid in the peptidoglycan.
29465337	4	48	contain	contained	476:484	arg2	acid					614:617	the diamino acid	602:617	the diamino acid in the peptidoglycan	602:638	Strain N237T contained iso-C16 : 0 and C17 : 1ω8c as the major fatty acids, MK-9(H4) as the predominant isoprenoid quinone and meso-DAP as the diamino acid in the peptidoglycan.
29465337	4	48	contain	contained	476:484	arg1	N237T					470:474	Strain N237T	463:474	Strain N237T	463:474	Strain N237T contained iso-C16 : 0 and C17 : 1ω8c as the major fatty acids, MK-9(H4) as the predominant isoprenoid quinone and meso-DAP as the diamino acid in the peptidoglycan.
29465337	4	48	contain	contained	476:484	arg2	quinone					578:584	the predominant isoprenoid quinone	551:584	the predominant isoprenoid quinone	551:584	Strain N237T contained iso-C16 : 0 and C17 : 1ω8c as the major fatty acids, MK-9(H4) as the predominant isoprenoid quinone and meso-DAP as the diamino acid in the peptidoglycan.
29465337	9	49	theme	chemotaxonomic	1215:1228	arg1	characteristics					1247:1261	The combined phenotypic, chemotaxonomic and phylogenetic characteristics	1190:1261	The combined phenotypic, chemotaxonomic and phylogenetic characteristics	1190:1261	The combined phenotypic, chemotaxonomic and phylogenetic characteristics supported the conclusion that strain N237T represents a novel species in the genus Jatrophihabitans, for which the name Jatrophihabitans telluris sp.
29465337	2	50	from	Dongbaek-Dongsan	257:272	arg1	wetlands					232:239	wetlands	232:239	wetlands at Meonmulkkak, Dongbaek-Dongsan, the lava forest, Gotjawal, Jeju, Republic of Korea	232:324	A novel actinobacterial strain, designated N237T, was isolated from sediment soil of wetlands at Meonmulkkak, Dongbaek-Dongsan, the lava forest, Gotjawal, Jeju, Republic of Korea.
29465337	2	50	from	Dongbaek-Dongsan	257:272	arg1	soil					224:227	sediment soil	215:227	sediment soil of wetlands at Meonmulkkak, Dongbaek-Dongsan, the lava forest, Gotjawal, Jeju, Republic of Korea	215:324	A novel actinobacterial strain, designated N237T, was isolated from sediment soil of wetlands at Meonmulkkak, Dongbaek-Dongsan, the lava forest, Gotjawal, Jeju, Republic of Korea.
29465337	9	51	theme	phylogenetic	1234:1245	arg1	characteristics					1247:1261	The combined phenotypic, chemotaxonomic and phylogenetic characteristics	1190:1261	The combined phenotypic, chemotaxonomic and phylogenetic characteristics	1190:1261	The combined phenotypic, chemotaxonomic and phylogenetic characteristics supported the conclusion that strain N237T represents a novel species in the genus Jatrophihabitans, for which the name Jatrophihabitans telluris sp.
29465337	7	52	theme	separate	978:985	arg1	lineage					987:993	a separate lineage	976:993	a separate lineage in the genus Jatrophihabitans	976:1023	Strain N237T formed a separate lineage in the genus Jatrophihabitans, as demonstrated by phylogenetic analysis based on 16S rRNA sequencing.
29465337	7	53	theme	genus	1002:1006	arg1	Jatrophihabitans					1008:1023	the genus Jatrophihabitans	998:1023	the genus Jatrophihabitans	998:1023	Strain N237T formed a separate lineage in the genus Jatrophihabitans, as demonstrated by phylogenetic analysis based on 16S rRNA sequencing.
29465337	5	54	theme	unidentified	884:895	arg1	lipids					897:902	two unidentified lipids	880:902	two unidentified lipids as polar lipids	880:918	It contained diphosphatidylglycerol, phosphatidylinositol polymannosides, an unidentified phospholipid, an unidentified aminophospholipid, an unidentified aminolipid, two unidentified glycophospholipids, three unidentified glycolipids and two unidentified lipids as polar lipids.
29465337	0	55	dep	sp	26:27	arg1	Jatrophihabitans					0:15	Jatrophihabitans	0:15	Jatrophihabitans	0:15	Jatrophihabitans telluris sp.
29465337	1	56	theme	Jatrophihabitans	129:144	arg1	soil					59:62	sediment soil	50:62	sediment soil of lava forest wetlands	50:86	nov., isolated from sediment soil of lava forest wetlands and the emended description of the genus Jatrophihabitans.
29465337	1	56	theme	Jatrophihabitans	129:144	arg1	description					104:114	the emended description	92:114	the emended description of the genus Jatrophihabitans	92:144	nov., isolated from sediment soil of lava forest wetlands and the emended description of the genus Jatrophihabitans.
29465337	11	57	theme	=KCTC	1457:1461	arg1	B-65477T					1475:1482	=KCTC 39922T=NRRL B-65477T	1457:1482	=KCTC 39922T=NRRL B-65477T	1457:1482	The type strain is N237T (=KCTC 39922T=NRRL B-65477T).
29465337	11	57	theme	=KCTC	1457:1461	arg1	N237T					1450:1454	N237T	1450:1454	N237T (=KCTC 39922T=NRRL B-65477T)	1450:1483	The type strain is N237T (=KCTC 39922T=NRRL B-65477T).
29465337	9	58	theme	name	1378:1381	arg1	sp					1409:1410	the name Jatrophihabitans telluris sp	1374:1410	the name Jatrophihabitans telluris sp	1374:1410	The combined phenotypic, chemotaxonomic and phylogenetic characteristics supported the conclusion that strain N237T represents a novel species in the genus Jatrophihabitans, for which the name Jatrophihabitans telluris sp.
29465337	5	59	theme	phosphatidylinositol	678:697	arg1	polymannosides					699:712	phosphatidylinositol polymannosides	678:712	phosphatidylinositol polymannosides	678:712	It contained diphosphatidylglycerol, phosphatidylinositol polymannosides, an unidentified phospholipid, an unidentified aminophospholipid, an unidentified aminolipid, two unidentified glycophospholipids, three unidentified glycolipids and two unidentified lipids as polar lipids.
29465337	2	60	theme	wetlands	232:239	arg1	soil					224:227	sediment soil	215:227	sediment soil of wetlands at Meonmulkkak, Dongbaek-Dongsan, the lava forest, Gotjawal, Jeju, Republic of Korea	215:324	A novel actinobacterial strain, designated N237T, was isolated from sediment soil of wetlands at Meonmulkkak, Dongbaek-Dongsan, the lava forest, Gotjawal, Jeju, Republic of Korea.
29465337	1	61	theme	genus	123:127	arg1	Jatrophihabitans					129:144	the genus Jatrophihabitans	119:144	the genus Jatrophihabitans	119:144	nov., isolated from sediment soil of lava forest wetlands and the emended description of the genus Jatrophihabitans.
29465337	4	62	theme	isoprenoid	567:576	arg1	quinone					578:584	the predominant isoprenoid quinone	551:584	the predominant isoprenoid quinone	551:584	Strain N237T contained iso-C16 : 0 and C17 : 1ω8c as the major fatty acids, MK-9(H4) as the predominant isoprenoid quinone and meso-DAP as the diamino acid in the peptidoglycan.
29465337	4	62	theme	isoprenoid	567:576	arg1	iso-C16 					486:493	iso-C16 	486:493	iso-C16 	486:493	Strain N237T contained iso-C16 : 0 and C17 : 1ω8c as the major fatty acids, MK-9(H4) as the predominant isoprenoid quinone and meso-DAP as the diamino acid in the peptidoglycan.
29465337	9	63	theme	Jatrophihabitans	1383:1398	arg1	sp					1409:1410	the name Jatrophihabitans telluris sp	1374:1410	the name Jatrophihabitans telluris sp	1374:1410	The combined phenotypic, chemotaxonomic and phylogenetic characteristics supported the conclusion that strain N237T represents a novel species in the genus Jatrophihabitans, for which the name Jatrophihabitans telluris sp.
29465337	4	64	theme	Strain	463:468	arg1	N237T					470:474	Strain N237T	463:474	Strain N237T	463:474	Strain N237T contained iso-C16 : 0 and C17 : 1ω8c as the major fatty acids, MK-9(H4) as the predominant isoprenoid quinone and meso-DAP as the diamino acid in the peptidoglycan.
29465337	8	65	theme	%	1166:1166	arg1	similarity					1177:1186	95.6 % sequence similarity	1161:1186	95.6 % sequence similarity	1161:1186	It was most closely related to Jatrophihabitans soli KIS75-12T (95.6 % sequence similarity).
29465337	8	65	theme	%	1166:1166	arg1	KIS75-12T					1150:1158	Jatrophihabitans soli KIS75-12T	1128:1158	Jatrophihabitans soli KIS75-12T (95.6 % sequence similarity)	1128:1187	It was most closely related to Jatrophihabitans soli KIS75-12T (95.6 % sequence similarity).
29465337	0	66	theme	telluris	17:24	arg1	sp					26:27	telluris sp	17:27	telluris sp	17:27	Jatrophihabitans telluris sp.
29465337	4	67	from	acid	614:617	arg1	peptidoglycan					626:638	the peptidoglycan	622:638	the peptidoglycan	622:638	Strain N237T contained iso-C16 : 0 and C17 : 1ω8c as the major fatty acids, MK-9(H4) as the predominant isoprenoid quinone and meso-DAP as the diamino acid in the peptidoglycan.
29465337	9	68	theme	telluris	1400:1407	arg1	sp					1409:1410	the name Jatrophihabitans telluris sp	1374:1410	the name Jatrophihabitans telluris sp	1374:1410	The combined phenotypic, chemotaxonomic and phylogenetic characteristics supported the conclusion that strain N237T represents a novel species in the genus Jatrophihabitans, for which the name Jatrophihabitans telluris sp.
30926385	9	0	theme	charged	1451:1457	arg1	acids					1465:1469	free electrically charged amino acids	1433:1469	free electrically charged amino acids	1433:1469	Moreover, this binding capacity was dependent on the pH value and inhibited by free electrically charged amino acids.
30926385	6	1	theme	tryptic	942:948	arg1	digestion					950:958	tryptic digestion	942:958	tryptic digestion	942:958	The trimeric H6HA1 was resistant to tryptic digestion; however, it could not bind to fetuin, a glycoprotein containing sialylated N-linked and O-linked glycans.
30926385	10	2	theme	influenza	1516:1524	arg1	virus					1526:1530	the influenza virus	1512:1530	the influenza virus with a host cell	1512:1547	We propose a two-step model for binding the influenza virus with a host cell.
30926385	12	3	theme	cell	1752:1755	arg1	surface					1757:1763	the cell surface	1748:1763	the cell surface	1748:1763	After the virus is engulfed by the acidic endosome, R201 could bind to the cell surface with stronger interactions and trigger the fusion process.
30926385	11	4	from	site	1623:1626	arg1	HA					1631:1632	HA	1631:1632	HA	1631:1632	The first step involved the specific recognition of the receptor binding site on HA to the sialylated glycan on the host cell.
30926385	4	5	theme	full-length	729:739	arg1	HA					741:742	nearly full-length HA	722:742	nearly full-length HA	722:742	To explore the role of R201 and the protecting glycan, we developed a bi-cistronic baculovirus expression system for the production of H6HA1 and H6HA0 (nearly full-length HA), which were glycosylated in insect cells.
30926385	4	5	theme	full-length	729:739	arg1	H6HA1					705:709	H6HA1	705:709	H6HA1	705:709	To explore the role of R201 and the protecting glycan, we developed a bi-cistronic baculovirus expression system for the production of H6HA1 and H6HA0 (nearly full-length HA), which were glycosylated in insect cells.
30926385	10	6	theme	host	1539:1542	arg1	cell					1544:1547	a host cell	1537:1547	a host cell	1537:1547	We propose a two-step model for binding the influenza virus with a host cell.
30926385	11	7	theme	receptor	1606:1613	arg1	site					1623:1626	the receptor binding site	1602:1626	the receptor binding site on HA to the sialylated glycan on the host cell	1602:1674	The first step involved the specific recognition of the receptor binding site on HA to the sialylated glycan on the host cell.
30926385	4	8	gly	glycosylated	757:768	arg1	production					691:700	the production	687:700	the production	687:700	To explore the role of R201 and the protecting glycan, we developed a bi-cistronic baculovirus expression system for the production of H6HA1 and H6HA0 (nearly full-length HA), which were glycosylated in insect cells.
30926385	4	8	gly	glycosylated	757:768	arg1	cells					780:784	insect cells	773:784	insect cells	773:784	To explore the role of R201 and the protecting glycan, we developed a bi-cistronic baculovirus expression system for the production of H6HA1 and H6HA0 (nearly full-length HA), which were glycosylated in insect cells.
30926385	4	8	gly	glycosylated	757:768	arg2	production					691:700	the production	687:700	the production	687:700	To explore the role of R201 and the protecting glycan, we developed a bi-cistronic baculovirus expression system for the production of H6HA1 and H6HA0 (nearly full-length HA), which were glycosylated in insect cells.
30926385	11	9	theme	specific	1578:1585	arg1	recognition					1587:1597	the specific recognition	1574:1597	the specific recognition of the receptor binding site on HA to the sialylated glycan on the host cell	1574:1674	The first step involved the specific recognition of the receptor binding site on HA to the sialylated glycan on the host cell.
30926385	12	10	theme	acidic	1712:1717	arg1	endosome					1719:1726	the acidic endosome	1708:1726	the acidic endosome	1708:1726	After the virus is engulfed by the acidic endosome, R201 could bind to the cell surface with stronger interactions and trigger the fusion process.
30926385	2	11	from	Asn-167	404:410	arg1	at					401:402	the glycan at Asn-167	390:410	the glycan at Asn-167 (N167)	390:417	The residue Arg-201 (R201) on this epitope was protected by the glycan at Asn-167 (N167) from tryptic digestion; therefore, the infectivity of the virus was retained.
30926385	11	12	theme	site	1623:1626	arg1	recognition					1587:1597	the specific recognition	1574:1597	the specific recognition of the receptor binding site on HA to the sialylated glycan on the host cell	1574:1674	The first step involved the specific recognition of the receptor binding site on HA to the sialylated glycan on the host cell.
30926385	4	13	theme	insect	773:778	arg1	cells					780:784	insect cells	773:784	insect cells	773:784	To explore the role of R201 and the protecting glycan, we developed a bi-cistronic baculovirus expression system for the production of H6HA1 and H6HA0 (nearly full-length HA), which were glycosylated in insect cells.
30926385	8	14	theme	host	1268:1271	arg1	cells					1273:1277	host cells	1268:1277	host cells	1268:1277	We found that the positive charge on R201 was critical for binding HA to the negatively charged surface of host cells because the mutant R201A of H6HA0 lost its binding capacity substantially.
30926385	6	15	link	O-linked	1049:1056	arg1	glycans					1058:1064	sialylated N-linked and O-linked glycans	1025:1064	sialylated N-linked and O-linked glycans	1025:1064	The trimeric H6HA1 was resistant to tryptic digestion; however, it could not bind to fetuin, a glycoprotein containing sialylated N-linked and O-linked glycans.
30926385	5	16	theme	monomeric	890:898	arg1	form					900:903	the monomeric form	886:903	the monomeric form	886:903	The expressed H6HA1 was mostly found in the trimeric form, and the H6HA0 protein was only found in the monomeric form.
30926385	5	17	theme	expressed	791:799	arg1	H6HA1					801:805	The expressed H6HA1	787:805	The expressed H6HA1	787:805	The expressed H6HA1 was mostly found in the trimeric form, and the H6HA0 protein was only found in the monomeric form.
30926385	6	18	theme	O-linked	1049:1056	arg1	glycans					1058:1064	sialylated N-linked and O-linked glycans	1025:1064	sialylated N-linked and O-linked glycans	1025:1064	The trimeric H6HA1 was resistant to tryptic digestion; however, it could not bind to fetuin, a glycoprotein containing sialylated N-linked and O-linked glycans.
30926385	4	19	theme	glycan	617:622	arg1	role					585:588	the role	581:588	the role of R201 and the protecting glycan	581:622	To explore the role of R201 and the protecting glycan, we developed a bi-cistronic baculovirus expression system for the production of H6HA1 and H6HA0 (nearly full-length HA), which were glycosylated in insect cells.
30926385	3	20	theme	various	529:535	arg1	subtypes					537:544	various subtypes	529:544	various subtypes of the influenza virus	529:567	R201 was extremely conserved in various subtypes of the influenza virus.
30926385	8	21	theme	mutant	1291:1296	arg1	R201A					1298:1302	the mutant R201A	1287:1302	the mutant R201A of H6HA0	1287:1311	We found that the positive charge on R201 was critical for binding HA to the negatively charged surface of host cells because the mutant R201A of H6HA0 lost its binding capacity substantially.
30926385	6	22	theme	N-linked	1036:1043	arg1	glycans					1058:1064	sialylated N-linked and O-linked glycans	1025:1064	sialylated N-linked and O-linked glycans	1025:1064	The trimeric H6HA1 was resistant to tryptic digestion; however, it could not bind to fetuin, a glycoprotein containing sialylated N-linked and O-linked glycans.
30926385	12	23	theme	fusion	1808:1813	arg1	process					1815:1821	the fusion process	1804:1821	the fusion process	1804:1821	After the virus is engulfed by the acidic endosome, R201 could bind to the cell surface with stronger interactions and trigger the fusion process.
30926385	4	24	theme	R201	593:596	arg1	role					585:588	the role	581:588	the role of R201 and the protecting glycan	581:622	To explore the role of R201 and the protecting glycan, we developed a bi-cistronic baculovirus expression system for the production of H6HA1 and H6HA0 (nearly full-length HA), which were glycosylated in insect cells.
30926385	1	25	theme	H6N1	279:282	arg1	A/chicken/Taiwan/2838 V/00					301:326	A/chicken/Taiwan/2838 V/00	301:326	A/chicken/Taiwan/2838 V/00	301:326	In our previous study, we produced a monoclonal antibody EB2 that recognized an epitope in the HA1 domain on the hemagglutinin (HA) of H6N1 influenza virus (A/chicken/Taiwan/2838 V/00).
30926385	1	25	theme	H6N1	279:282	arg1	virus					294:298	H6N1 influenza virus	279:298	H6N1 influenza virus (A/chicken/Taiwan/2838 V/00)	279:327	In our previous study, we produced a monoclonal antibody EB2 that recognized an epitope in the HA1 domain on the hemagglutinin (HA) of H6N1 influenza virus (A/chicken/Taiwan/2838 V/00).
30926385	1	26	dep	antibody	192:199	arg1	EB2					201:203	EB2	201:203	a monoclonal antibody EB2 that recognized an epitope in the HA1 domain on the hemagglutinin (HA) of H6N1 influenza virus (A/chicken/Taiwan/2838 V/00)	179:327	In our previous study, we produced a monoclonal antibody EB2 that recognized an epitope in the HA1 domain on the hemagglutinin (HA) of H6N1 influenza virus (A/chicken/Taiwan/2838 V/00).
30926385	4	27	theme	baculovirus	653:663	arg1	system					676:681	a bi-cistronic baculovirus expression system	638:681	a bi-cistronic baculovirus expression system for the production of H6HA1 and H6HA0 (nearly full-length HA), which were glycosylated in insect cells	638:784	To explore the role of R201 and the protecting glycan, we developed a bi-cistronic baculovirus expression system for the production of H6HA1 and H6HA0 (nearly full-length HA), which were glycosylated in insect cells.
30926385	11	28	theme	first	1554:1558	arg1	step					1560:1563	The first step	1550:1563	The first step	1550:1563	The first step involved the specific recognition of the receptor binding site on HA to the sialylated glycan on the host cell.
30926385	0	29	theme	H6N1	75:78	arg1	virus					96:100	H6N1 avian influenza virus	75:100	H6N1 avian influenza virus	75:100	Positive charge of Arg-201 on hemagglutinin is required for the binding of H6N1 avian influenza virus to its target through a two-step process.
30926385	1	30	theme	virus	294:298	arg1	hemagglutinin					257:269	the hemagglutinin	253:269	the hemagglutinin (HA) of H6N1 influenza virus (A/chicken/Taiwan/2838 V/00)	253:327	In our previous study, we produced a monoclonal antibody EB2 that recognized an epitope in the HA1 domain on the hemagglutinin (HA) of H6N1 influenza virus (A/chicken/Taiwan/2838 V/00).
30926385	1	30	theme	virus	294:298	arg1	HA					272:273	HA	272:273	HA	272:273	In our previous study, we produced a monoclonal antibody EB2 that recognized an epitope in the HA1 domain on the hemagglutinin (HA) of H6N1 influenza virus (A/chicken/Taiwan/2838 V/00).
30926385	2	31	theme	tryptic	424:430	arg1	digestion					432:440	tryptic digestion	424:440	tryptic digestion	424:440	The residue Arg-201 (R201) on this epitope was protected by the glycan at Asn-167 (N167) from tryptic digestion; therefore, the infectivity of the virus was retained.
30926385	8	32	theme	binding	1322:1328	arg1	capacity					1330:1337	its binding capacity	1318:1337	its binding capacity	1318:1337	We found that the positive charge on R201 was critical for binding HA to the negatively charged surface of host cells because the mutant R201A of H6HA0 lost its binding capacity substantially.
30926385	8	33	theme	positive	1179:1186	arg1	critical					1207:1214	critical	1207:1214	critical	1207:1214	We found that the positive charge on R201 was critical for binding HA to the negatively charged surface of host cells because the mutant R201A of H6HA0 lost its binding capacity substantially.
30926385	8	33	theme	positive	1179:1186	arg1	charge					1188:1193	the positive charge	1175:1193	the positive charge on R201	1175:1201	We found that the positive charge on R201 was critical for binding HA to the negatively charged surface of host cells because the mutant R201A of H6HA0 lost its binding capacity substantially.
30926385	0	34	theme	influenza	86:94	arg1	virus					96:100	H6N1 avian influenza virus	75:100	H6N1 avian influenza virus	75:100	Positive charge of Arg-201 on hemagglutinin is required for the binding of H6N1 avian influenza virus to its target through a two-step process.
30926385	9	35	theme	binding	1369:1375	arg1	capacity					1377:1384	this binding capacity	1364:1384	this binding capacity	1364:1384	Moreover, this binding capacity was dependent on the pH value and inhibited by free electrically charged amino acids.
30926385	0	36	theme	Positive	0:7	arg1	charge					9:14	Positive charge	0:14	Positive charge of Arg-201 on hemagglutinin	0:42	Positive charge of Arg-201 on hemagglutinin is required for the binding of H6N1 avian influenza virus to its target through a two-step process.
30926385	6	37	theme	trimeric	910:917	arg1	H6HA1					919:923	The trimeric H6HA1	906:923	The trimeric H6HA1	906:923	The trimeric H6HA1 was resistant to tryptic digestion; however, it could not bind to fetuin, a glycoprotein containing sialylated N-linked and O-linked glycans.
30926385	6	37	theme	trimeric	910:917	arg1	resistant					929:937	resistant	929:937	resistant	929:937	The trimeric H6HA1 was resistant to tryptic digestion; however, it could not bind to fetuin, a glycoprotein containing sialylated N-linked and O-linked glycans.
30926385	10	38	theme	two-step	1485:1492	arg1	model					1494:1498	a two-step model	1483:1498	a two-step model for binding the influenza virus with a host cell	1483:1547	We propose a two-step model for binding the influenza virus with a host cell.
30926385	2	39	theme	at	401:402	arg1	N167					413:416	N167	413:416	N167	413:416	The residue Arg-201 (R201) on this epitope was protected by the glycan at Asn-167 (N167) from tryptic digestion; therefore, the infectivity of the virus was retained.
30926385	2	39	theme	at	401:402	arg1	Asn-167					404:410	the glycan at Asn-167	390:410	the glycan at Asn-167 (N167)	390:417	The residue Arg-201 (R201) on this epitope was protected by the glycan at Asn-167 (N167) from tryptic digestion; therefore, the infectivity of the virus was retained.
30926385	3	40	theme	influenza	553:561	arg1	virus					563:567	the influenza virus	549:567	the influenza virus	549:567	R201 was extremely conserved in various subtypes of the influenza virus.
30926385	5	41	located	found	818:822	arg1	form					840:843	the trimeric form	827:843	the trimeric form	827:843	The expressed H6HA1 was mostly found in the trimeric form, and the H6HA0 protein was only found in the monomeric form.
30926385	5	41	located	found	818:822	arg2	H6HA1					801:805	The expressed H6HA1	787:805	The expressed H6HA1	787:805	The expressed H6HA1 was mostly found in the trimeric form, and the H6HA0 protein was only found in the monomeric form.
30926385	9	42	theme	pH	1407:1408	arg1	value					1410:1414	the pH value	1403:1414	the pH value	1403:1414	Moreover, this binding capacity was dependent on the pH value and inhibited by free electrically charged amino acids.
30926385	8	43	theme	HA	1228:1229	arg1	binding					1220:1226	binding HA	1220:1229	binding HA to the negatively charged surface of host cells	1220:1277	We found that the positive charge on R201 was critical for binding HA to the negatively charged surface of host cells because the mutant R201A of H6HA0 lost its binding capacity substantially.
30926385	10	44	with	virus	1526:1530	arg1	cell					1544:1547	a host cell	1537:1547	a host cell	1537:1547	We propose a two-step model for binding the influenza virus with a host cell.
30926385	2	45	from	Arg-201	342:348	arg1	epitope					365:371	this epitope	360:371	this epitope	360:371	The residue Arg-201 (R201) on this epitope was protected by the glycan at Asn-167 (N167) from tryptic digestion; therefore, the infectivity of the virus was retained.
30926385	1	46	theme	monoclonal	181:190	arg1	antibody					192:199	a monoclonal antibody	179:199	a monoclonal antibody EB2 that recognized an epitope in the HA1 domain on the hemagglutinin (HA) of H6N1 influenza virus (A/chicken/Taiwan/2838 V/00)	179:327	In our previous study, we produced a monoclonal antibody EB2 that recognized an epitope in the HA1 domain on the hemagglutinin (HA) of H6N1 influenza virus (A/chicken/Taiwan/2838 V/00).
30926385	5	47	theme	H6HA0	854:858	arg1	protein					860:866	the H6HA0 protein	850:866	the H6HA0 protein	850:866	The expressed H6HA1 was mostly found in the trimeric form, and the H6HA0 protein was only found in the monomeric form.
30926385	8	48	theme	charged	1249:1255	arg1	surface					1257:1263	the negatively charged surface	1234:1263	the negatively charged surface of host cells	1234:1277	We found that the positive charge on R201 was critical for binding HA to the negatively charged surface of host cells because the mutant R201A of H6HA0 lost its binding capacity substantially.
30926385	12	49	theme	stronger	1770:1777	arg1	interactions					1779:1790	stronger interactions	1770:1790	stronger interactions	1770:1790	After the virus is engulfed by the acidic endosome, R201 could bind to the cell surface with stronger interactions and trigger the fusion process.
30926385	6	50	contain	containing	1014:1023	arg1	fetuin					991:996	fetuin	991:996	fetuin	991:996	The trimeric H6HA1 was resistant to tryptic digestion; however, it could not bind to fetuin, a glycoprotein containing sialylated N-linked and O-linked glycans.
30926385	6	50	contain	containing	1014:1023	arg1	glycoprotein					1001:1012	a glycoprotein	999:1012	a glycoprotein containing sialylated N-linked and O-linked glycans	999:1064	The trimeric H6HA1 was resistant to tryptic digestion; however, it could not bind to fetuin, a glycoprotein containing sialylated N-linked and O-linked glycans.
30926385	6	50	contain	containing	1014:1023	arg2	glycans					1058:1064	sialylated N-linked and O-linked glycans	1025:1064	sialylated N-linked and O-linked glycans	1025:1064	The trimeric H6HA1 was resistant to tryptic digestion; however, it could not bind to fetuin, a glycoprotein containing sialylated N-linked and O-linked glycans.
30926385	6	51	gly	sialylated	1025:1034	arg1	glycans					1058:1064	sialylated N-linked and O-linked glycans	1025:1064	sialylated N-linked and O-linked glycans	1025:1064	The trimeric H6HA1 was resistant to tryptic digestion; however, it could not bind to fetuin, a glycoprotein containing sialylated N-linked and O-linked glycans.
30926385	9	52	theme	amino	1459:1463	arg1	acids					1465:1469	free electrically charged amino acids	1433:1469	free electrically charged amino acids	1433:1469	Moreover, this binding capacity was dependent on the pH value and inhibited by free electrically charged amino acids.
30926385	8	53	from	charge	1188:1193	arg1	R201					1198:1201	R201	1198:1201	R201	1198:1201	We found that the positive charge on R201 was critical for binding HA to the negatively charged surface of host cells because the mutant R201A of H6HA0 lost its binding capacity substantially.
30926385	11	54	from	recognition	1587:1597	arg1	HA					1631:1632	HA	1631:1632	HA	1631:1632	The first step involved the specific recognition of the receptor binding site on HA to the sialylated glycan on the host cell.
30926385	1	55	theme	previous	151:158	arg1	study					160:164	our previous study	147:164	our previous study	147:164	In our previous study, we produced a monoclonal antibody EB2 that recognized an epitope in the HA1 domain on the hemagglutinin (HA) of H6N1 influenza virus (A/chicken/Taiwan/2838 V/00).
30926385	1	56	from	epitope	224:230	arg1	domain					243:248	the HA1 domain	235:248	the HA1 domain	235:248	In our previous study, we produced a monoclonal antibody EB2 that recognized an epitope in the HA1 domain on the hemagglutinin (HA) of H6N1 influenza virus (A/chicken/Taiwan/2838 V/00).
30926385	1	56	from	epitope	224:230	arg1	hemagglutinin					257:269	the hemagglutinin	253:269	the hemagglutinin (HA) of H6N1 influenza virus (A/chicken/Taiwan/2838 V/00)	253:327	In our previous study, we produced a monoclonal antibody EB2 that recognized an epitope in the HA1 domain on the hemagglutinin (HA) of H6N1 influenza virus (A/chicken/Taiwan/2838 V/00).
30926385	1	56	from	epitope	224:230	arg1	HA					272:273	HA	272:273	HA	272:273	In our previous study, we produced a monoclonal antibody EB2 that recognized an epitope in the HA1 domain on the hemagglutinin (HA) of H6N1 influenza virus (A/chicken/Taiwan/2838 V/00).
30926385	6	57	gly	glycoprotein	1001:1012	arg1	fetuin					991:996	fetuin	991:996	fetuin	991:996	The trimeric H6HA1 was resistant to tryptic digestion; however, it could not bind to fetuin, a glycoprotein containing sialylated N-linked and O-linked glycans.
30926385	6	57	gly	glycoprotein	1001:1012	arg1	glycoprotein					1001:1012	a glycoprotein	999:1012	a glycoprotein containing sialylated N-linked and O-linked glycans	999:1064	The trimeric H6HA1 was resistant to tryptic digestion; however, it could not bind to fetuin, a glycoprotein containing sialylated N-linked and O-linked glycans.
30926385	8	58	theme	cells	1273:1277	arg1	surface					1257:1263	the negatively charged surface	1234:1263	the negatively charged surface of host cells	1234:1277	We found that the positive charge on R201 was critical for binding HA to the negatively charged surface of host cells because the mutant R201A of H6HA0 lost its binding capacity substantially.
30926385	0	59	theme	two-step	126:133	arg1	process					135:141	a two-step process	124:141	a two-step process	124:141	Positive charge of Arg-201 on hemagglutinin is required for the binding of H6N1 avian influenza virus to its target through a two-step process.
30926385	2	60	theme	virus	477:481	arg1	infectivity					458:468	the infectivity	454:468	the infectivity of the virus	454:481	The residue Arg-201 (R201) on this epitope was protected by the glycan at Asn-167 (N167) from tryptic digestion; therefore, the infectivity of the virus was retained.
30926385	11	61	gly	sialylated	1641:1650	arg1	glycan					1652:1657	the sialylated glycan	1637:1657	the sialylated glycan on the host cell	1637:1674	The first step involved the specific recognition of the receptor binding site on HA to the sialylated glycan on the host cell.
30926385	11	62	theme	binding	1615:1621	arg1	site					1623:1626	the receptor binding site	1602:1626	the receptor binding site on HA to the sialylated glycan on the host cell	1602:1674	The first step involved the specific recognition of the receptor binding site on HA to the sialylated glycan on the host cell.
30926385	4	63	theme	protecting	606:615	arg1	glycan					617:622	the protecting glycan	602:622	the protecting glycan	602:622	To explore the role of R201 and the protecting glycan, we developed a bi-cistronic baculovirus expression system for the production of H6HA1 and H6HA0 (nearly full-length HA), which were glycosylated in insect cells.
30926385	8	64	theme	H6HA0	1307:1311	arg1	R201A					1298:1302	the mutant R201A	1287:1302	the mutant R201A of H6HA0	1287:1311	We found that the positive charge on R201 was critical for binding HA to the negatively charged surface of host cells because the mutant R201A of H6HA0 lost its binding capacity substantially.
30926385	6	65	theme	sialylated	1025:1034	arg1	glycans					1058:1064	sialylated N-linked and O-linked glycans	1025:1064	sialylated N-linked and O-linked glycans	1025:1064	The trimeric H6HA1 was resistant to tryptic digestion; however, it could not bind to fetuin, a glycoprotein containing sialylated N-linked and O-linked glycans.
30926385	5	66	located	found	877:881	arg1	form					900:903	the monomeric form	886:903	the monomeric form	886:903	The expressed H6HA1 was mostly found in the trimeric form, and the H6HA0 protein was only found in the monomeric form.
30926385	5	66	located	found	877:881	arg2	protein					860:866	the H6HA0 protein	850:866	the H6HA0 protein	850:866	The expressed H6HA1 was mostly found in the trimeric form, and the H6HA0 protein was only found in the monomeric form.
30926385	0	67	from	charge	9:14	arg1	hemagglutinin					30:42	hemagglutinin	30:42	hemagglutinin	30:42	Positive charge of Arg-201 on hemagglutinin is required for the binding of H6N1 avian influenza virus to its target through a two-step process.
30926385	11	68	from	glycan	1652:1657	arg1	cell					1671:1674	the host cell	1662:1674	the host cell	1662:1674	The first step involved the specific recognition of the receptor binding site on HA to the sialylated glycan on the host cell.
30926385	0	69	theme	avian	80:84	arg1	virus					96:100	H6N1 avian influenza virus	75:100	H6N1 avian influenza virus	75:100	Positive charge of Arg-201 on hemagglutinin is required for the binding of H6N1 avian influenza virus to its target through a two-step process.
30926385	1	70	theme	influenza	284:292	arg1	A/chicken/Taiwan/2838 V/00					301:326	A/chicken/Taiwan/2838 V/00	301:326	A/chicken/Taiwan/2838 V/00	301:326	In our previous study, we produced a monoclonal antibody EB2 that recognized an epitope in the HA1 domain on the hemagglutinin (HA) of H6N1 influenza virus (A/chicken/Taiwan/2838 V/00).
30926385	1	70	theme	influenza	284:292	arg1	virus					294:298	H6N1 influenza virus	279:298	H6N1 influenza virus (A/chicken/Taiwan/2838 V/00)	279:327	In our previous study, we produced a monoclonal antibody EB2 that recognized an epitope in the HA1 domain on the hemagglutinin (HA) of H6N1 influenza virus (A/chicken/Taiwan/2838 V/00).
30926385	5	71	theme	trimeric	831:838	arg1	form					840:843	the trimeric form	827:843	the trimeric form	827:843	The expressed H6HA1 was mostly found in the trimeric form, and the H6HA0 protein was only found in the monomeric form.
30926385	4	72	theme	bi-cistronic	640:651	arg1	system					676:681	a bi-cistronic baculovirus expression system	638:681	a bi-cistronic baculovirus expression system for the production of H6HA1 and H6HA0 (nearly full-length HA), which were glycosylated in insect cells	638:784	To explore the role of R201 and the protecting glycan, we developed a bi-cistronic baculovirus expression system for the production of H6HA1 and H6HA0 (nearly full-length HA), which were glycosylated in insect cells.
30926385	0	73	theme	virus	96:100	arg1	binding					64:70	the binding	60:70	the binding of H6N1 avian influenza virus to its target through a two-step process	60:141	Positive charge of Arg-201 on hemagglutinin is required for the binding of H6N1 avian influenza virus to its target through a two-step process.
30926385	11	74	from	HA	1631:1632	arg1	recognition					1587:1597	the specific recognition	1574:1597	the specific recognition of the receptor binding site on HA to the sialylated glycan on the host cell	1574:1674	The first step involved the specific recognition of the receptor binding site on HA to the sialylated glycan on the host cell.
30926385	4	75	theme	expression	665:674	arg1	system					676:681	a bi-cistronic baculovirus expression system	638:681	a bi-cistronic baculovirus expression system for the production of H6HA1 and H6HA0 (nearly full-length HA), which were glycosylated in insect cells	638:784	To explore the role of R201 and the protecting glycan, we developed a bi-cistronic baculovirus expression system for the production of H6HA1 and H6HA0 (nearly full-length HA), which were glycosylated in insect cells.
30926385	2	76	theme	residue	334:340	arg1	R201					351:354	R201	351:354	R201	351:354	The residue Arg-201 (R201) on this epitope was protected by the glycan at Asn-167 (N167) from tryptic digestion; therefore, the infectivity of the virus was retained.
30926385	2	76	theme	residue	334:340	arg1	Arg-201					342:348	The residue Arg-201	330:348	The residue Arg-201 (R201) on this epitope	330:371	The residue Arg-201 (R201) on this epitope was protected by the glycan at Asn-167 (N167) from tryptic digestion; therefore, the infectivity of the virus was retained.
30926385	7	77	theme	tryptic	1142:1148	arg1	digestion					1150:1158	tryptic digestion	1142:1158	tryptic digestion	1142:1158	By contrast, the monomeric H6HA0 could bind to fetuin but was sensitive to tryptic digestion.
30926385	0	78	theme	Arg-201	19:25	arg1	charge					9:14	Positive charge	0:14	Positive charge of Arg-201 on hemagglutinin	0:42	Positive charge of Arg-201 on hemagglutinin is required for the binding of H6N1 avian influenza virus to its target through a two-step process.
30926385	1	79	theme	HA1	239:241	arg1	domain					243:248	the HA1 domain	235:248	the HA1 domain	235:248	In our previous study, we produced a monoclonal antibody EB2 that recognized an epitope in the HA1 domain on the hemagglutinin (HA) of H6N1 influenza virus (A/chicken/Taiwan/2838 V/00).
30926385	2	80	theme	glycan	394:399	arg1	N167					413:416	N167	413:416	N167	413:416	The residue Arg-201 (R201) on this epitope was protected by the glycan at Asn-167 (N167) from tryptic digestion; therefore, the infectivity of the virus was retained.
30926385	2	80	theme	glycan	394:399	arg1	Asn-167					404:410	the glycan at Asn-167	390:410	the glycan at Asn-167 (N167)	390:417	The residue Arg-201 (R201) on this epitope was protected by the glycan at Asn-167 (N167) from tryptic digestion; therefore, the infectivity of the virus was retained.
30926385	4	81	theme	H6HA1	705:709	arg1	production					691:700	the production	687:700	the production	687:700	To explore the role of R201 and the protecting glycan, we developed a bi-cistronic baculovirus expression system for the production of H6HA1 and H6HA0 (nearly full-length HA), which were glycosylated in insect cells.
30926385	6	82	link	N-linked	1036:1043	arg1	glycans					1058:1064	sialylated N-linked and O-linked glycans	1025:1064	sialylated N-linked and O-linked glycans	1025:1064	The trimeric H6HA1 was resistant to tryptic digestion; however, it could not bind to fetuin, a glycoprotein containing sialylated N-linked and O-linked glycans.
30926385	4	83	theme	H6HA0	715:719	arg1	production					691:700	the production	687:700	the production	687:700	To explore the role of R201 and the protecting glycan, we developed a bi-cistronic baculovirus expression system for the production of H6HA1 and H6HA0 (nearly full-length HA), which were glycosylated in insect cells.
30926385	11	84	theme	sialylated	1641:1650	arg1	glycan					1652:1657	the sialylated glycan	1637:1657	the sialylated glycan on the host cell	1637:1674	The first step involved the specific recognition of the receptor binding site on HA to the sialylated glycan on the host cell.
30926385	3	85	theme	virus	563:567	arg1	subtypes					537:544	various subtypes	529:544	various subtypes of the influenza virus	529:567	R201 was extremely conserved in various subtypes of the influenza virus.
30926385	9	86	theme	free	1433:1436	arg1	acids					1465:1469	free electrically charged amino acids	1433:1469	free electrically charged amino acids	1433:1469	Moreover, this binding capacity was dependent on the pH value and inhibited by free electrically charged amino acids.
30926385	7	87	theme	monomeric	1084:1092	arg1	sensitive					1129:1137	sensitive	1129:1137	sensitive	1129:1137	By contrast, the monomeric H6HA0 could bind to fetuin but was sensitive to tryptic digestion.
30926385	7	87	theme	monomeric	1084:1092	arg1	H6HA0					1094:1098	the monomeric H6HA0	1080:1098	the monomeric H6HA0	1080:1098	By contrast, the monomeric H6HA0 could bind to fetuin but was sensitive to tryptic digestion.
30926385	11	88	theme	host	1666:1669	arg1	cell					1671:1674	the host cell	1662:1674	the host cell	1662:1674	The first step involved the specific recognition of the receptor binding site on HA to the sialylated glycan on the host cell.
31527716	0	0	theme	microbiota	81:90	arg1	diversity					92:100	the faecal microbiota diversity	70:100	the faecal microbiota diversity	70:100	Lactulose drives a reversible reduction and qualitative modulation of the faecal microbiota diversity in healthy dogs.
31527716	5	1	theme	oral	703:706	arg1	lactulose					708:716	added oral lactulose	697:716	added oral lactulose in weeks 6-7	697:729	Faecal samples were collected weekly, while the subjects were either on their usual diet (week 1), or a standardised diet (weeks 2-9), with added oral lactulose in weeks 6-7.
31527716	5	2	theme	usual	635:639	arg1	diet					641:644	their usual diet	629:644	their usual diet (week 1)	629:653	Faecal samples were collected weekly, while the subjects were either on their usual diet (week 1), or a standardised diet (weeks 2-9), with added oral lactulose in weeks 6-7.
31527716	5	2	theme	usual	635:639	arg1	week					647:650	week 1	647:652	week 1	647:652	Faecal samples were collected weekly, while the subjects were either on their usual diet (week 1), or a standardised diet (weeks 2-9), with added oral lactulose in weeks 6-7.
31527716	7	3	theme	Shannon/Chao1	924:936	arg1	indexes					938:944	Shannon/Chao1 indexes	924:944	Shannon/Chao1 indexes	924:944	Faecal samples from week 7 had a significantly lower microbiota richness/diversity, based on observed operational taxonomic units, Shannon/Chao1 indexes and Pielou's Evenness.
31527716	4	4	theme	Healthy	480:486	arg1	dogs					505:508	Healthy, privately owned dogs	480:508	Healthy, privately owned dogs (n = 18)	480:517	Healthy, privately owned dogs (n = 18) completed a prospective cohort study.
31527716	4	4	theme	Healthy	480:486	arg1	n = 18					511:516	n = 18	511:516	n = 18	511:516	Healthy, privately owned dogs (n = 18) completed a prospective cohort study.
31527716	0	5	theme	faecal	74:79	arg1	diversity					92:100	the faecal microbiota diversity	70:100	the faecal microbiota diversity	70:100	Lactulose drives a reversible reduction and qualitative modulation of the faecal microbiota diversity in healthy dogs.
31527716	8	6	theme	Beta	969:972	arg1	diversity					974:982	Beta diversity	969:982	Beta diversity based on UniFrac distances	969:1009	Beta diversity based on UniFrac distances was significantly different in week 7 compared to weeks 1, 5 and 9.
31527716	6	7	theme	16S	751:753	arg1	sequencing					765:774	16S rRNA gene sequencing	751:774	16S rRNA gene sequencing	751:774	DNA extraction and 16S rRNA gene sequencing were undertaken.
31527716	1	8	theme	debilitating	160:171	arg1	encephalopathy					127:140	Hepatic encephalopathy	119:140	Hepatic encephalopathy	119:140	Hepatic encephalopathy is a frequent and debilitating complication of liver disorders.
31527716	1	8	theme	debilitating	160:171	arg1	complication					173:184	a frequent and debilitating complication	145:184	a frequent and debilitating complication of liver disorders	145:203	Hepatic encephalopathy is a frequent and debilitating complication of liver disorders.
31527716	10	9	theme	qualitative	1328:1338	arg1	change					1357:1362	a reversible qualitative and quantitative change	1315:1362	a reversible qualitative and quantitative change	1315:1362	In summary, we have shown that lactulose induces a reversible qualitative and quantitative change of the faecal microbiota, which may explain its clinical efficacy in the management of hepatic encephalopathy.
31527716	9	10	theme	Bacteroidetes	1202:1214	arg1	increase					1141:1148	a significant increase	1127:1148	a significant increase of Firmicutes and Actinobacteria	1127:1181	At the phylum level, week 7 was associated with a significant increase of Firmicutes and Actinobacteria, and a decrease of Bacteroidetes and Fusobacteria, when compared to weeks 5 and 9.
31527716	9	10	theme	Bacteroidetes	1202:1214	arg1	decrease					1190:1197	a decrease	1188:1197	a decrease of Bacteroidetes and Fusobacteria, when compared to weeks 5 and 9	1188:1263	At the phylum level, week 7 was associated with a significant increase of Firmicutes and Actinobacteria, and a decrease of Bacteroidetes and Fusobacteria, when compared to weeks 5 and 9.
31527716	7	11	contain	had	820:822	arg2	richness/diversity					857:874	a significantly lower microbiota richness/diversity	824:874	a significantly lower microbiota richness/diversity	824:874	Faecal samples from week 7 had a significantly lower microbiota richness/diversity, based on observed operational taxonomic units, Shannon/Chao1 indexes and Pielou's Evenness.
31527716	7	11	contain	had	820:822	arg1	samples					800:806	Faecal samples	793:806	Faecal samples from week 7	793:818	Faecal samples from week 7 had a significantly lower microbiota richness/diversity, based on observed operational taxonomic units, Shannon/Chao1 indexes and Pielou's Evenness.
31527716	9	12	theme	phylum	1086:1091	arg1	level					1093:1097	the phylum level	1082:1097	the phylum level	1082:1097	At the phylum level, week 7 was associated with a significant increase of Firmicutes and Actinobacteria, and a decrease of Bacteroidetes and Fusobacteria, when compared to weeks 5 and 9.
31527716	0	13	theme	diversity	92:100	arg1	reduction					30:38	a reversible reduction	17:38	a reversible reduction	17:38	Lactulose drives a reversible reduction and qualitative modulation of the faecal microbiota diversity in healthy dogs.
31527716	0	13	theme	diversity	92:100	arg1	modulation					56:65	qualitative modulation	44:65	qualitative modulation of the faecal microbiota diversity in healthy dogs	44:116	Lactulose drives a reversible reduction and qualitative modulation of the faecal microbiota diversity in healthy dogs.
31527716	0	14	from	modulation	56:65	arg1	dogs					113:116	healthy dogs	105:116	healthy dogs	105:116	Lactulose drives a reversible reduction and qualitative modulation of the faecal microbiota diversity in healthy dogs.
31527716	7	15	theme	observed	886:893	arg1	units					917:921	observed operational taxonomic units	886:921	observed operational taxonomic units	886:921	Faecal samples from week 7 had a significantly lower microbiota richness/diversity, based on observed operational taxonomic units, Shannon/Chao1 indexes and Pielou's Evenness.
31527716	7	16	from	week	813:816	arg1	samples					800:806	Faecal samples	793:806	Faecal samples from week 7	793:818	Faecal samples from week 7 had a significantly lower microbiota richness/diversity, based on observed operational taxonomic units, Shannon/Chao1 indexes and Pielou's Evenness.
31527716	7	17	theme	Faecal	793:798	arg1	samples					800:806	Faecal samples	793:806	Faecal samples from week 7	793:818	Faecal samples from week 7 had a significantly lower microbiota richness/diversity, based on observed operational taxonomic units, Shannon/Chao1 indexes and Pielou's Evenness.
31527716	10	18	theme	hepatic	1451:1457	arg1	encephalopathy					1459:1472	hepatic encephalopathy	1451:1472	hepatic encephalopathy	1451:1472	In summary, we have shown that lactulose induces a reversible qualitative and quantitative change of the faecal microbiota, which may explain its clinical efficacy in the management of hepatic encephalopathy.
31527716	9	19	theme	Fusobacteria	1220:1231	arg1	increase					1141:1148	a significant increase	1127:1148	a significant increase of Firmicutes and Actinobacteria	1127:1181	At the phylum level, week 7 was associated with a significant increase of Firmicutes and Actinobacteria, and a decrease of Bacteroidetes and Fusobacteria, when compared to weeks 5 and 9.
31527716	9	19	theme	Fusobacteria	1220:1231	arg1	decrease					1190:1197	a decrease	1188:1197	a decrease of Bacteroidetes and Fusobacteria, when compared to weeks 5 and 9	1188:1263	At the phylum level, week 7 was associated with a significant increase of Firmicutes and Actinobacteria, and a decrease of Bacteroidetes and Fusobacteria, when compared to weeks 5 and 9.
31527716	6	20	theme	DNA	732:734	arg1	extraction					736:745	DNA extraction	732:745	DNA extraction	732:745	DNA extraction and 16S rRNA gene sequencing were undertaken.
31527716	2	21	theme	understood	292:301	arg1	mechanisms					303:312	incompletely understood mechanisms	279:312	incompletely understood mechanisms of action	279:322	Lactulose is an established and reasonably effective treatment, yet with incompletely understood mechanisms of action.
31527716	0	22	theme	healthy	105:111	arg1	dogs					113:116	healthy dogs	105:116	healthy dogs	105:116	Lactulose drives a reversible reduction and qualitative modulation of the faecal microbiota diversity in healthy dogs.
31527716	8	23	theme	UniFrac	993:999	arg1	distances					1001:1009	UniFrac distances	993:1009	UniFrac distances	993:1009	Beta diversity based on UniFrac distances was significantly different in week 7 compared to weeks 1, 5 and 9.
31527716	0	24	from	reduction	30:38	arg1	dogs					113:116	healthy dogs	105:116	healthy dogs	105:116	Lactulose drives a reversible reduction and qualitative modulation of the faecal microbiota diversity in healthy dogs.
31527716	10	25	theme	encephalopathy	1459:1472	arg1	management					1437:1446	the management	1433:1446	the management of hepatic encephalopathy	1433:1472	In summary, we have shown that lactulose induces a reversible qualitative and quantitative change of the faecal microbiota, which may explain its clinical efficacy in the management of hepatic encephalopathy.
31527716	1	26	theme	liver	189:193	arg1	disorders					195:203	liver disorders	189:203	liver disorders	189:203	Hepatic encephalopathy is a frequent and debilitating complication of liver disorders.
31527716	10	27	theme	quantitative	1344:1355	arg1	change					1357:1362	a reversible qualitative and quantitative change	1315:1362	a reversible qualitative and quantitative change	1315:1362	In summary, we have shown that lactulose induces a reversible qualitative and quantitative change of the faecal microbiota, which may explain its clinical efficacy in the management of hepatic encephalopathy.
31527716	3	28	theme	lactulose	435:443	arg1	treatment					445:453	lactulose treatment	435:453	lactulose treatment	435:453	The aims of this study were to examine how the faecal microbiota composition changed before, during and after lactulose treatment in a large animal model.
31527716	3	29	theme	faecal	372:377	arg1	composition					390:400	the faecal microbiota composition	368:400	the faecal microbiota composition	368:400	The aims of this study were to examine how the faecal microbiota composition changed before, during and after lactulose treatment in a large animal model.
31527716	4	30	theme	cohort	543:548	arg1	study					550:554	a prospective cohort study	529:554	a prospective cohort study	529:554	Healthy, privately owned dogs (n = 18) completed a prospective cohort study.
31527716	10	31	theme	reversible	1317:1326	arg1	change					1357:1362	a reversible qualitative and quantitative change	1315:1362	a reversible qualitative and quantitative change	1315:1362	In summary, we have shown that lactulose induces a reversible qualitative and quantitative change of the faecal microbiota, which may explain its clinical efficacy in the management of hepatic encephalopathy.
31527716	7	32	theme	operational	895:905	arg1	units					917:921	observed operational taxonomic units	886:921	observed operational taxonomic units	886:921	Faecal samples from week 7 had a significantly lower microbiota richness/diversity, based on observed operational taxonomic units, Shannon/Chao1 indexes and Pielou's Evenness.
31527716	7	33	theme	taxonomic	907:915	arg1	units					917:921	observed operational taxonomic units	886:921	observed operational taxonomic units	886:921	Faecal samples from week 7 had a significantly lower microbiota richness/diversity, based on observed operational taxonomic units, Shannon/Chao1 indexes and Pielou's Evenness.
31527716	3	34	theme	animal	466:471	arg1	model					473:477	a large animal model	458:477	a large animal model	458:477	The aims of this study were to examine how the faecal microbiota composition changed before, during and after lactulose treatment in a large animal model.
31527716	3	35	theme	microbiota	379:388	arg1	composition					390:400	the faecal microbiota composition	368:400	the faecal microbiota composition	368:400	The aims of this study were to examine how the faecal microbiota composition changed before, during and after lactulose treatment in a large animal model.
31527716	4	36	dep	Healthy	480:486	arg1	owned					499:503	owned	499:503	owned	499:503	Healthy, privately owned dogs (n = 18) completed a prospective cohort study.
31527716	5	37	theme	standardised	661:672	arg1	weeks					680:684	weeks 2-9	680:688	weeks 2-9	680:688	Faecal samples were collected weekly, while the subjects were either on their usual diet (week 1), or a standardised diet (weeks 2-9), with added oral lactulose in weeks 6-7.
31527716	5	37	theme	standardised	661:672	arg1	diet					674:677	a standardised diet	659:677	a standardised diet (weeks 2-9)	659:689	Faecal samples were collected weekly, while the subjects were either on their usual diet (week 1), or a standardised diet (weeks 2-9), with added oral lactulose in weeks 6-7.
31527716	9	38	theme	significant	1129:1139	arg1	increase					1141:1148	a significant increase	1127:1148	a significant increase of Firmicutes and Actinobacteria	1127:1181	At the phylum level, week 7 was associated with a significant increase of Firmicutes and Actinobacteria, and a decrease of Bacteroidetes and Fusobacteria, when compared to weeks 5 and 9.
31527716	2	39	theme	action	317:322	arg1	mechanisms					303:312	incompletely understood mechanisms	279:312	incompletely understood mechanisms of action	279:322	Lactulose is an established and reasonably effective treatment, yet with incompletely understood mechanisms of action.
31527716	10	40	theme	microbiota	1378:1387	arg1	change					1357:1362	a reversible qualitative and quantitative change	1315:1362	a reversible qualitative and quantitative change	1315:1362	In summary, we have shown that lactulose induces a reversible qualitative and quantitative change of the faecal microbiota, which may explain its clinical efficacy in the management of hepatic encephalopathy.
31527716	2	41	theme	effective	249:257	arg1	treatment					259:267	reasonably effective treatment	238:267	reasonably effective treatment	238:267	Lactulose is an established and reasonably effective treatment, yet with incompletely understood mechanisms of action.
31527716	1	42	theme	disorders	195:203	arg1	encephalopathy					127:140	Hepatic encephalopathy	119:140	Hepatic encephalopathy	119:140	Hepatic encephalopathy is a frequent and debilitating complication of liver disorders.
31527716	1	42	theme	disorders	195:203	arg1	complication					173:184	a frequent and debilitating complication	145:184	a frequent and debilitating complication of liver disorders	145:203	Hepatic encephalopathy is a frequent and debilitating complication of liver disorders.
31527716	10	43	theme	faecal	1371:1376	arg1	microbiota					1378:1387	the faecal microbiota	1367:1387	the faecal microbiota	1367:1387	In summary, we have shown that lactulose induces a reversible qualitative and quantitative change of the faecal microbiota, which may explain its clinical efficacy in the management of hepatic encephalopathy.
31527716	0	44	theme	reversible	19:28	arg1	reduction					30:38	a reversible reduction	17:38	a reversible reduction	17:38	Lactulose drives a reversible reduction and qualitative modulation of the faecal microbiota diversity in healthy dogs.
31527716	3	45	theme	large	460:464	arg1	model					473:477	a large animal model	458:477	a large animal model	458:477	The aims of this study were to examine how the faecal microbiota composition changed before, during and after lactulose treatment in a large animal model.
31527716	10	46	theme	clinical	1412:1419	arg1	efficacy					1421:1428	its clinical efficacy	1408:1428	its clinical efficacy	1408:1428	In summary, we have shown that lactulose induces a reversible qualitative and quantitative change of the faecal microbiota, which may explain its clinical efficacy in the management of hepatic encephalopathy.
31527716	9	47	theme	Firmicutes	1153:1162	arg1	increase					1141:1148	a significant increase	1127:1148	a significant increase of Firmicutes and Actinobacteria	1127:1181	At the phylum level, week 7 was associated with a significant increase of Firmicutes and Actinobacteria, and a decrease of Bacteroidetes and Fusobacteria, when compared to weeks 5 and 9.
31527716	9	47	theme	Firmicutes	1153:1162	arg1	decrease					1190:1197	a decrease	1188:1197	a decrease of Bacteroidetes and Fusobacteria, when compared to weeks 5 and 9	1188:1263	At the phylum level, week 7 was associated with a significant increase of Firmicutes and Actinobacteria, and a decrease of Bacteroidetes and Fusobacteria, when compared to weeks 5 and 9.
31527716	0	48	theme	qualitative	44:54	arg1	modulation					56:65	qualitative modulation	44:65	qualitative modulation of the faecal microbiota diversity in healthy dogs	44:116	Lactulose drives a reversible reduction and qualitative modulation of the faecal microbiota diversity in healthy dogs.
31527716	4	49	theme	prospective	531:541	arg1	study					550:554	a prospective cohort study	529:554	a prospective cohort study	529:554	Healthy, privately owned dogs (n = 18) completed a prospective cohort study.
31527716	9	50	theme	Actinobacteria	1168:1181	arg1	increase					1141:1148	a significant increase	1127:1148	a significant increase of Firmicutes and Actinobacteria	1127:1181	At the phylum level, week 7 was associated with a significant increase of Firmicutes and Actinobacteria, and a decrease of Bacteroidetes and Fusobacteria, when compared to weeks 5 and 9.
31527716	9	50	theme	Actinobacteria	1168:1181	arg1	decrease					1190:1197	a decrease	1188:1197	a decrease of Bacteroidetes and Fusobacteria, when compared to weeks 5 and 9	1188:1263	At the phylum level, week 7 was associated with a significant increase of Firmicutes and Actinobacteria, and a decrease of Bacteroidetes and Fusobacteria, when compared to weeks 5 and 9.
31527716	1	51	theme	Hepatic	119:125	arg1	encephalopathy					127:140	Hepatic encephalopathy	119:140	Hepatic encephalopathy	119:140	Hepatic encephalopathy is a frequent and debilitating complication of liver disorders.
31527716	1	51	theme	Hepatic	119:125	arg1	complication					173:184	a frequent and debilitating complication	145:184	a frequent and debilitating complication of liver disorders	145:203	Hepatic encephalopathy is a frequent and debilitating complication of liver disorders.
31527716	7	52	theme	lower	840:844	arg1	richness/diversity					857:874	a significantly lower microbiota richness/diversity	824:874	a significantly lower microbiota richness/diversity	824:874	Faecal samples from week 7 had a significantly lower microbiota richness/diversity, based on observed operational taxonomic units, Shannon/Chao1 indexes and Pielou's Evenness.
31527716	5	53	dep	weeks	680:684	arg1	2-9					686:688	2-9	686:688	2-9	686:688	Faecal samples were collected weekly, while the subjects were either on their usual diet (week 1), or a standardised diet (weeks 2-9), with added oral lactulose in weeks 6-7.
31527716	5	54	from	lactulose	708:716	arg1	weeks					721:725	weeks 6-7	721:729	weeks 6-7	721:729	Faecal samples were collected weekly, while the subjects were either on their usual diet (week 1), or a standardised diet (weeks 2-9), with added oral lactulose in weeks 6-7.
31527716	5	55	theme	Faecal	557:562	arg1	samples					564:570	Faecal samples	557:570	Faecal samples	557:570	Faecal samples were collected weekly, while the subjects were either on their usual diet (week 1), or a standardised diet (weeks 2-9), with added oral lactulose in weeks 6-7.
31527716	7	56	theme	microbiota	846:855	arg1	richness/diversity					857:874	a significantly lower microbiota richness/diversity	824:874	a significantly lower microbiota richness/diversity	824:874	Faecal samples from week 7 had a significantly lower microbiota richness/diversity, based on observed operational taxonomic units, Shannon/Chao1 indexes and Pielou's Evenness.
31527716	6	57	theme	gene	760:763	arg1	sequencing					765:774	16S rRNA gene sequencing	751:774	16S rRNA gene sequencing	751:774	DNA extraction and 16S rRNA gene sequencing were undertaken.
31527716	8	58	from	week	1042:1045	arg1	different					1029:1037	different	1029:1037	different	1029:1037	Beta diversity based on UniFrac distances was significantly different in week 7 compared to weeks 1, 5 and 9.
31527716	3	59	theme	study	342:346	arg1	aims					329:332	The aims	325:332	The aims of this study	325:346	The aims of this study were to examine how the faecal microbiota composition changed before, during and after lactulose treatment in a large animal model.
31527716	6	60	theme	rRNA	755:758	arg1	sequencing					765:774	16S rRNA gene sequencing	751:774	16S rRNA gene sequencing	751:774	DNA extraction and 16S rRNA gene sequencing were undertaken.
31527716	5	61	theme	added	697:701	arg1	lactulose					708:716	added oral lactulose	697:716	added oral lactulose in weeks 6-7	697:729	Faecal samples were collected weekly, while the subjects were either on their usual diet (week 1), or a standardised diet (weeks 2-9), with added oral lactulose in weeks 6-7.
31527716	1	62	theme	frequent	147:154	arg1	encephalopathy					127:140	Hepatic encephalopathy	119:140	Hepatic encephalopathy	119:140	Hepatic encephalopathy is a frequent and debilitating complication of liver disorders.
31527716	1	62	theme	frequent	147:154	arg1	complication					173:184	a frequent and debilitating complication	145:184	a frequent and debilitating complication of liver disorders	145:203	Hepatic encephalopathy is a frequent and debilitating complication of liver disorders.
31527716	8	63	from	different	1029:1037	arg1	week					1042:1045	week 7	1042:1047	week 7	1042:1047	Beta diversity based on UniFrac distances was significantly different in week 7 compared to weeks 1, 5 and 9.
30265231	7	0	theme	isoprenoid	1058:1067	arg1	quinones					1069:1076	the dominant isoprenoid quinones	1045:1076	the dominant isoprenoid quinones	1045:1076	The principal fatty acids were anteiso-C15 : 0, C16 : 1ω7c alcohol, iso-C16 : 0 and iso-C14 : 0, and the dominant isoprenoid quinones were MK-8 and MK-7.
30265231	7	0	theme	isoprenoid	1058:1067	arg1	MK-8					1083:1086	MK-8	1083:1086	MK-8	1083:1086	The principal fatty acids were anteiso-C15 : 0, C16 : 1ω7c alcohol, iso-C16 : 0 and iso-C14 : 0, and the dominant isoprenoid quinones were MK-8 and MK-7.
30265231	4	1	theme	Phylogenetic	473:484	arg1	trees					486:490	Phylogenetic trees	473:490	Phylogenetic trees as well as diagnostic signature nucleotides in the 16S rRNA gene sequence	473:564	Phylogenetic trees as well as diagnostic signature nucleotides in the 16S rRNA gene sequence supported the view that this strain should be assigned to the genus Planococcus.
30265231	7	2	theme	 1ω7c	997:1001	arg1	iso-C16 					1012:1019	iso-C16 	1012:1019	iso-C16 	1012:1019	The principal fatty acids were anteiso-C15 : 0, C16 : 1ω7c alcohol, iso-C16 : 0 and iso-C14 : 0, and the dominant isoprenoid quinones were MK-8 and MK-7.
30265231	7	2	theme	 1ω7c	997:1001	arg1	alcohol					1003:1009	 1ω7c alcohol	997:1009	 1ω7c alcohol	997:1009	The principal fatty acids were anteiso-C15 : 0, C16 : 1ω7c alcohol, iso-C16 : 0 and iso-C14 : 0, and the dominant isoprenoid quinones were MK-8 and MK-7.
30265231	10	3	theme	genotypic	1375:1383	arg1	data					1416:1419	the genotypic, phenotypic and chemotaxonomic data	1371:1419	the genotypic, phenotypic and chemotaxonomic data	1371:1419	Based on the genotypic, phenotypic and chemotaxonomic data, strain SCU63T can be classified as a novel species in the genus Planococcus, for which the name Planococcushalotolerans sp.
30265231	1	4	from	sample	63:68	arg1	China					73:77	China	73:77	China	73:77	nov., isolated from a saline soil sample in China.
30265231	6	5	theme	w/v	927:929	arg1	NaCl					932:935	0-15 % (w/v) NaCl	919:935	0-15 % (w/v) NaCl (3 %)	919:941	The isolate grew at 0-40 °C (optimum, 30-35 °C), at pH 6.5-9.0 (pH 7.0-7.5) and in the presence of 0-15 % (w/v) NaCl (3 %).
30265231	6	5	theme	w/v	927:929	arg1	%					940:940	3 %	938:940	3 %	938:940	The isolate grew at 0-40 °C (optimum, 30-35 °C), at pH 6.5-9.0 (pH 7.0-7.5) and in the presence of 0-15 % (w/v) NaCl (3 %).
30265231	10	6	theme	chemotaxonomic	1401:1414	arg1	data					1416:1419	the genotypic, phenotypic and chemotaxonomic data	1371:1419	the genotypic, phenotypic and chemotaxonomic data	1371:1419	Based on the genotypic, phenotypic and chemotaxonomic data, strain SCU63T can be classified as a novel species in the genus Planococcus, for which the name Planococcushalotolerans sp.
30265231	2	7	theme	Gram-stain-positive	88:106	arg1	bacterium					198:206	A novel Gram-stain-positive, coccoid or short rod-shaped, moderate-orange-pigmented, halotolerant and psychrotolerant bacterium	80:206	A novel Gram-stain-positive, coccoid or short rod-shaped, moderate-orange-pigmented, halotolerant and psychrotolerant bacterium	80:206	A novel Gram-stain-positive, coccoid or short rod-shaped, moderate-orange-pigmented, halotolerant and psychrotolerant bacterium, designated strain SCU63T, was isolated from a saline soil sample in China, and characterized by a polyphasic taxonomic approach.
30265231	4	8	theme	gene	552:555	arg1	sequence					557:564	the 16S rRNA gene sequence	539:564	the 16S rRNA gene sequence	539:564	Phylogenetic trees as well as diagnostic signature nucleotides in the 16S rRNA gene sequence supported the view that this strain should be assigned to the genus Planococcus.
30265231	3	9	theme	rRNA	342:345	arg1	similarity					361:370	16S rRNA gene sequence similarity	338:370	16S rRNA gene sequence similarity of strain SCU63T to species in the genera Planococcus and Planomicrobium	338:443	16S rRNA gene sequence similarity of strain SCU63T to species in the genera Planococcus and Planomicrobium ranged from 96.5 to 98.6 %.
30265231	2	10	theme	novel	82:86	arg1	bacterium					198:206	A novel Gram-stain-positive, coccoid or short rod-shaped, moderate-orange-pigmented, halotolerant and psychrotolerant bacterium	80:206	A novel Gram-stain-positive, coccoid or short rod-shaped, moderate-orange-pigmented, halotolerant and psychrotolerant bacterium	80:206	A novel Gram-stain-positive, coccoid or short rod-shaped, moderate-orange-pigmented, halotolerant and psychrotolerant bacterium, designated strain SCU63T, was isolated from a saline soil sample in China, and characterized by a polyphasic taxonomic approach.
30265231	4	11	from	trees	486:490	arg1	sequence					557:564	the 16S rRNA gene sequence	539:564	the 16S rRNA gene sequence	539:564	Phylogenetic trees as well as diagnostic signature nucleotides in the 16S rRNA gene sequence supported the view that this strain should be assigned to the genus Planococcus.
30265231	8	12	theme	polar	1169:1173	arg1	lipids					1175:1180	the polar lipids	1165:1180	the polar lipids	1165:1180	The peptidoglycan type was determined to be A4α (l-Lys-d-Glu), and the polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unidentified aminophospholipid and one unidentified lipid.
30265231	6	13	theme	%	924:924	arg1	NaCl					932:935	0-15 % (w/v) NaCl	919:935	0-15 % (w/v) NaCl (3 %)	919:941	The isolate grew at 0-40 °C (optimum, 30-35 °C), at pH 6.5-9.0 (pH 7.0-7.5) and in the presence of 0-15 % (w/v) NaCl (3 %).
30265231	6	13	theme	%	924:924	arg1	%					940:940	3 %	938:940	3 %	938:940	The isolate grew at 0-40 °C (optimum, 30-35 °C), at pH 6.5-9.0 (pH 7.0-7.5) and in the presence of 0-15 % (w/v) NaCl (3 %).
30265231	3	14	theme	gene	347:350	arg1	similarity					361:370	16S rRNA gene sequence similarity	338:370	16S rRNA gene sequence similarity of strain SCU63T to species in the genera Planococcus and Planomicrobium	338:443	16S rRNA gene sequence similarity of strain SCU63T to species in the genera Planococcus and Planomicrobium ranged from 96.5 to 98.6 %.
30265231	3	15	dep	98.6 	465:469	arg1	to					462:463	to	462:463	to	462:463	16S rRNA gene sequence similarity of strain SCU63T to species in the genera Planococcus and Planomicrobium ranged from 96.5 to 98.6 %.
30265231	1	16	theme	saline	51:56	arg1	sample					63:68	a saline soil sample	49:68	a saline soil sample in China	49:77	nov., isolated from a saline soil sample in China.
30265231	3	17	from	similarity	361:370	arg1	genera					407:412	the genera Planococcus and Planomicrobium	403:443	the genera Planococcus and Planomicrobium	403:443	16S rRNA gene sequence similarity of strain SCU63T to species in the genera Planococcus and Planomicrobium ranged from 96.5 to 98.6 %.
30265231	1	18	theme	soil	58:61	arg1	sample					63:68	a saline soil sample	49:68	a saline soil sample in China	49:77	nov., isolated from a saline soil sample in China.
30265231	4	19	theme	genus	628:632	arg1	Planococcus					634:644	the genus Planococcus	624:644	the genus Planococcus	624:644	Phylogenetic trees as well as diagnostic signature nucleotides in the 16S rRNA gene sequence supported the view that this strain should be assigned to the genus Planococcus.
30265231	3	20	theme	16S	338:340	arg1	similarity					361:370	16S rRNA gene sequence similarity	338:370	16S rRNA gene sequence similarity of strain SCU63T to species in the genera Planococcus and Planomicrobium	338:443	16S rRNA gene sequence similarity of strain SCU63T to species in the genera Planococcus and Planomicrobium ranged from 96.5 to 98.6 %.
30265231	2	21	theme	short	120:124	arg1	bacterium					198:206	A novel Gram-stain-positive, coccoid or short rod-shaped, moderate-orange-pigmented, halotolerant and psychrotolerant bacterium	80:206	A novel Gram-stain-positive, coccoid or short rod-shaped, moderate-orange-pigmented, halotolerant and psychrotolerant bacterium	80:206	A novel Gram-stain-positive, coccoid or short rod-shaped, moderate-orange-pigmented, halotolerant and psychrotolerant bacterium, designated strain SCU63T, was isolated from a saline soil sample in China, and characterized by a polyphasic taxonomic approach.
30265231	5	22	theme	strain	768:773	arg1	SCU63T					775:780	strain SCU63T	768:780	strain SCU63T	768:780	Further, average nucleotide identity and digital DNA-DNA hybridization analyses confirmed the separate species status of strain SCU63T relative to the closely related taxa.
30265231	2	23	theme	soil	262:265	arg1	sample					267:272	a saline soil sample	253:272	a saline soil sample in China	253:281	A novel Gram-stain-positive, coccoid or short rod-shaped, moderate-orange-pigmented, halotolerant and psychrotolerant bacterium, designated strain SCU63T, was isolated from a saline soil sample in China, and characterized by a polyphasic taxonomic approach.
30265231	8	24	contain	contained	1182:1190	arg2	phosphatidylethanolamine					1238:1261	phosphatidylethanolamine	1238:1261	phosphatidylethanolamine	1238:1261	The peptidoglycan type was determined to be A4α (l-Lys-d-Glu), and the polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unidentified aminophospholipid and one unidentified lipid.
30265231	8	24	contain	contained	1182:1190	arg2	diphosphatidylglycerol					1192:1213	diphosphatidylglycerol	1192:1213	diphosphatidylglycerol	1192:1213	The peptidoglycan type was determined to be A4α (l-Lys-d-Glu), and the polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unidentified aminophospholipid and one unidentified lipid.
30265231	8	24	contain	contained	1182:1190	arg1	lipids					1175:1180	the polar lipids	1165:1180	the polar lipids	1165:1180	The peptidoglycan type was determined to be A4α (l-Lys-d-Glu), and the polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unidentified aminophospholipid and one unidentified lipid.
30265231	8	24	contain	contained	1182:1190	arg2	lipid					1320:1324	one unidentified lipid	1303:1324	one unidentified lipid	1303:1324	The peptidoglycan type was determined to be A4α (l-Lys-d-Glu), and the polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unidentified aminophospholipid and one unidentified lipid.
30265231	8	24	contain	contained	1182:1190	arg2	phosphatidylglycerol					1216:1235	phosphatidylglycerol	1216:1235	phosphatidylglycerol	1216:1235	The peptidoglycan type was determined to be A4α (l-Lys-d-Glu), and the polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unidentified aminophospholipid and one unidentified lipid.
30265231	8	24	contain	contained	1182:1190	arg2	aminophospholipid					1281:1297	aminophospholipid	1281:1297	aminophospholipid	1281:1297	The peptidoglycan type was determined to be A4α (l-Lys-d-Glu), and the polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unidentified aminophospholipid and one unidentified lipid.
30265231	10	25	theme	novel	1459:1463	arg1	species					1465:1471	a novel species	1457:1471	a novel species	1457:1471	Based on the genotypic, phenotypic and chemotaxonomic data, strain SCU63T can be classified as a novel species in the genus Planococcus, for which the name Planococcushalotolerans sp.
30265231	10	25	theme	novel	1459:1463	arg1	SCU63T					1429:1434	strain SCU63T	1422:1434	strain SCU63T	1422:1434	Based on the genotypic, phenotypic and chemotaxonomic data, strain SCU63T can be classified as a novel species in the genus Planococcus, for which the name Planococcushalotolerans sp.
30265231	10	26	theme	strain	1422:1427	arg1	species					1465:1471	a novel species	1457:1471	a novel species	1457:1471	Based on the genotypic, phenotypic and chemotaxonomic data, strain SCU63T can be classified as a novel species in the genus Planococcus, for which the name Planococcushalotolerans sp.
30265231	10	26	theme	strain	1422:1427	arg1	SCU63T					1429:1434	strain SCU63T	1422:1434	strain SCU63T	1422:1434	Based on the genotypic, phenotypic and chemotaxonomic data, strain SCU63T can be classified as a novel species in the genus Planococcus, for which the name Planococcushalotolerans sp.
30265231	3	27	theme	strain	375:380	arg1	SCU63T					382:387	strain SCU63T	375:387	strain SCU63T	375:387	16S rRNA gene sequence similarity of strain SCU63T to species in the genera Planococcus and Planomicrobium ranged from 96.5 to 98.6 %.
30265231	12	28	theme	1.13628T=KCTC	1598:1610	arg1	SCU63T					1583:1588	SCU63T	1583:1588	SCU63T (=CGMCC 1.13628T=KCTC 43001T)	1583:1618	The type strain is SCU63T (=CGMCC 1.13628T=KCTC 43001T).
30265231	12	28	theme	1.13628T=KCTC	1598:1610	arg1	43001T					1612:1617	=CGMCC 1.13628T=KCTC 43001T	1591:1617	=CGMCC 1.13628T=KCTC 43001T	1591:1617	The type strain is SCU63T (=CGMCC 1.13628T=KCTC 43001T).
30265231	5	29	theme	hybridization	704:716	arg1	analyses					718:725	average nucleotide identity and digital DNA-DNA hybridization analyses	656:725	average nucleotide identity and digital DNA-DNA hybridization analyses	656:725	Further, average nucleotide identity and digital DNA-DNA hybridization analyses confirmed the separate species status of strain SCU63T relative to the closely related taxa.
30265231	9	30	theme	DNA	1331:1333	arg1	G+C content					1335:1345	The DNA G+C content	1327:1345	The DNA G+C content	1327:1345	The DNA G+C content was 44.6 mol%.
30265231	9	30	theme	DNA	1331:1333	arg1	%					1359:1359	44.6 mol%	1351:1359	44.6 mol%	1351:1359	The DNA G+C content was 44.6 mol%.
30265231	3	31	theme	sequence	352:359	arg1	similarity					361:370	16S rRNA gene sequence similarity	338:370	16S rRNA gene sequence similarity of strain SCU63T to species in the genera Planococcus and Planomicrobium	338:443	16S rRNA gene sequence similarity of strain SCU63T to species in the genera Planococcus and Planomicrobium ranged from 96.5 to 98.6 %.
30265231	8	32	theme	unidentified	1307:1318	arg1	lipid					1320:1324	one unidentified lipid	1303:1324	one unidentified lipid	1303:1324	The peptidoglycan type was determined to be A4α (l-Lys-d-Glu), and the polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unidentified aminophospholipid and one unidentified lipid.
30265231	5	33	theme	identity	675:682	arg1	analyses					718:725	average nucleotide identity and digital DNA-DNA hybridization analyses	656:725	average nucleotide identity and digital DNA-DNA hybridization analyses	656:725	Further, average nucleotide identity and digital DNA-DNA hybridization analyses confirmed the separate species status of strain SCU63T relative to the closely related taxa.
30265231	12	34	theme	=CGMCC	1591:1596	arg1	SCU63T					1583:1588	SCU63T	1583:1588	SCU63T (=CGMCC 1.13628T=KCTC 43001T)	1583:1618	The type strain is SCU63T (=CGMCC 1.13628T=KCTC 43001T).
30265231	12	34	theme	=CGMCC	1591:1596	arg1	43001T					1612:1617	=CGMCC 1.13628T=KCTC 43001T	1591:1617	=CGMCC 1.13628T=KCTC 43001T	1591:1617	The type strain is SCU63T (=CGMCC 1.13628T=KCTC 43001T).
30265231	10	35	theme	name	1513:1516	arg1	sp					1542:1543	the name Planococcushalotolerans sp	1509:1543	the name Planococcushalotolerans sp	1509:1543	Based on the genotypic, phenotypic and chemotaxonomic data, strain SCU63T can be classified as a novel species in the genus Planococcus, for which the name Planococcushalotolerans sp.
30265231	4	36	theme	rRNA	547:550	arg1	sequence					557:564	the 16S rRNA gene sequence	539:564	the 16S rRNA gene sequence	539:564	Phylogenetic trees as well as diagnostic signature nucleotides in the 16S rRNA gene sequence supported the view that this strain should be assigned to the genus Planococcus.
30265231	6	37	theme	NaCl	932:935	arg1	presence					907:914	the presence	903:914	the presence of 0-15 % (w/v) NaCl (3 %)	903:941	The isolate grew at 0-40 °C (optimum, 30-35 °C), at pH 6.5-9.0 (pH 7.0-7.5) and in the presence of 0-15 % (w/v) NaCl (3 %).
30265231	8	38	theme	peptidoglycan	1102:1114	arg1	A4α					1142:1144	A4α	1142:1144	A4α (l-Lys-d-Glu)	1142:1158	The peptidoglycan type was determined to be A4α (l-Lys-d-Glu), and the polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unidentified aminophospholipid and one unidentified lipid.
30265231	8	38	theme	peptidoglycan	1102:1114	arg1	type					1116:1119	The peptidoglycan type	1098:1119	The peptidoglycan type	1098:1119	The peptidoglycan type was determined to be A4α (l-Lys-d-Glu), and the polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unidentified aminophospholipid and one unidentified lipid.
30265231	4	39	from	nucleotides	524:534	arg1	sequence					557:564	the 16S rRNA gene sequence	539:564	the 16S rRNA gene sequence	539:564	Phylogenetic trees as well as diagnostic signature nucleotides in the 16S rRNA gene sequence supported the view that this strain should be assigned to the genus Planococcus.
30265231	4	40	theme	16S	543:545	arg1	rRNA					547:550	16S rRNA	543:550	the 16S rRNA gene sequence	539:564	Phylogenetic trees as well as diagnostic signature nucleotides in the 16S rRNA gene sequence supported the view that this strain should be assigned to the genus Planococcus.
30265231	5	41	theme	digital	688:694	arg1	hybridization					704:716	digital DNA-DNA hybridization	688:716	digital DNA-DNA hybridization	688:716	Further, average nucleotide identity and digital DNA-DNA hybridization analyses confirmed the separate species status of strain SCU63T relative to the closely related taxa.
30265231	2	42	theme	halotolerant	165:176	arg1	bacterium					198:206	A novel Gram-stain-positive, coccoid or short rod-shaped, moderate-orange-pigmented, halotolerant and psychrotolerant bacterium	80:206	A novel Gram-stain-positive, coccoid or short rod-shaped, moderate-orange-pigmented, halotolerant and psychrotolerant bacterium	80:206	A novel Gram-stain-positive, coccoid or short rod-shaped, moderate-orange-pigmented, halotolerant and psychrotolerant bacterium, designated strain SCU63T, was isolated from a saline soil sample in China, and characterized by a polyphasic taxonomic approach.
30265231	9	43	theme	44.6 mol	1351:1358	arg1	G+C content					1335:1345	The DNA G+C content	1327:1345	The DNA G+C content	1327:1345	The DNA G+C content was 44.6 mol%.
30265231	9	43	theme	44.6 mol	1351:1358	arg1	%					1359:1359	44.6 mol%	1351:1359	44.6 mol%	1351:1359	The DNA G+C content was 44.6 mol%.
30265231	5	44	theme	SCU63T	775:780	arg1	status					758:763	the separate species status	737:763	the separate species status of strain SCU63T relative to the closely related taxa	737:817	Further, average nucleotide identity and digital DNA-DNA hybridization analyses confirmed the separate species status of strain SCU63T relative to the closely related taxa.
30265231	2	45	dep	Gram-stain-positive	88:106	arg1	rod-shaped					126:135	rod-shaped	126:135	rod-shaped	126:135	A novel Gram-stain-positive, coccoid or short rod-shaped, moderate-orange-pigmented, halotolerant and psychrotolerant bacterium, designated strain SCU63T, was isolated from a saline soil sample in China, and characterized by a polyphasic taxonomic approach.
30265231	5	46	theme	DNA-DNA	696:702	arg1	hybridization					704:716	digital DNA-DNA hybridization	688:716	digital DNA-DNA hybridization	688:716	Further, average nucleotide identity and digital DNA-DNA hybridization analyses confirmed the separate species status of strain SCU63T relative to the closely related taxa.
30265231	3	47	dep	genera	407:412	arg1	Planococcus					414:424	Planococcus	414:424	Planococcus	414:424	16S rRNA gene sequence similarity of strain SCU63T to species in the genera Planococcus and Planomicrobium ranged from 96.5 to 98.6 %.
30265231	3	47	dep	genera	407:412	arg1	Planomicrobium					430:443	Planomicrobium	430:443	Planomicrobium	430:443	16S rRNA gene sequence similarity of strain SCU63T to species in the genera Planococcus and Planomicrobium ranged from 96.5 to 98.6 %.
30265231	5	48	theme	related	806:812	arg1	taxa					814:817	the closely related taxa	794:817	the closely related taxa	794:817	Further, average nucleotide identity and digital DNA-DNA hybridization analyses confirmed the separate species status of strain SCU63T relative to the closely related taxa.
30265231	3	49	theme	SCU63T	382:387	arg1	similarity					361:370	16S rRNA gene sequence similarity	338:370	16S rRNA gene sequence similarity of strain SCU63T to species in the genera Planococcus and Planomicrobium	338:443	16S rRNA gene sequence similarity of strain SCU63T to species in the genera Planococcus and Planomicrobium ranged from 96.5 to 98.6 %.
30265231	4	50	theme	diagnostic	503:512	arg1	nucleotides					524:534	diagnostic signature nucleotides	503:534	Phylogenetic trees as well as diagnostic signature nucleotides in the 16S rRNA gene sequence	473:564	Phylogenetic trees as well as diagnostic signature nucleotides in the 16S rRNA gene sequence supported the view that this strain should be assigned to the genus Planococcus.
30265231	2	51	theme	moderate-orange-pigmented	138:162	arg1	bacterium					198:206	A novel Gram-stain-positive, coccoid or short rod-shaped, moderate-orange-pigmented, halotolerant and psychrotolerant bacterium	80:206	A novel Gram-stain-positive, coccoid or short rod-shaped, moderate-orange-pigmented, halotolerant and psychrotolerant bacterium	80:206	A novel Gram-stain-positive, coccoid or short rod-shaped, moderate-orange-pigmented, halotolerant and psychrotolerant bacterium, designated strain SCU63T, was isolated from a saline soil sample in China, and characterized by a polyphasic taxonomic approach.
30265231	7	52	theme	fatty	958:962	arg1	anteiso-C15 					975:986	anteiso-C15 	975:986	anteiso-C15 	975:986	The principal fatty acids were anteiso-C15 : 0, C16 : 1ω7c alcohol, iso-C16 : 0 and iso-C14 : 0, and the dominant isoprenoid quinones were MK-8 and MK-7.
30265231	7	52	theme	fatty	958:962	arg1	acids					964:968	The principal fatty acids	944:968	The principal fatty acids	944:968	The principal fatty acids were anteiso-C15 : 0, C16 : 1ω7c alcohol, iso-C16 : 0 and iso-C14 : 0, and the dominant isoprenoid quinones were MK-8 and MK-7.
30265231	6	53	dep	optimum	849:855	arg1	30-35 °C					858:865	30-35 °C	858:865	30-35 °C	858:865	The isolate grew at 0-40 °C (optimum, 30-35 °C), at pH 6.5-9.0 (pH 7.0-7.5) and in the presence of 0-15 % (w/v) NaCl (3 %).
30265231	10	54	theme	phenotypic	1386:1395	arg1	data					1416:1419	the genotypic, phenotypic and chemotaxonomic data	1371:1419	the genotypic, phenotypic and chemotaxonomic data	1371:1419	Based on the genotypic, phenotypic and chemotaxonomic data, strain SCU63T can be classified as a novel species in the genus Planococcus, for which the name Planococcushalotolerans sp.
30265231	10	55	theme	Planococcushalotolerans	1518:1540	arg1	sp					1542:1543	the name Planococcushalotolerans sp	1509:1543	the name Planococcushalotolerans sp	1509:1543	Based on the genotypic, phenotypic and chemotaxonomic data, strain SCU63T can be classified as a novel species in the genus Planococcus, for which the name Planococcushalotolerans sp.
30265231	2	56	theme	strain	220:225	arg1	SCU63T					227:232	strain SCU63T	220:232	strain SCU63T	220:232	A novel Gram-stain-positive, coccoid or short rod-shaped, moderate-orange-pigmented, halotolerant and psychrotolerant bacterium, designated strain SCU63T, was isolated from a saline soil sample in China, and characterized by a polyphasic taxonomic approach.
30265231	2	57	theme	taxonomic	318:326	arg1	approach					328:335	a polyphasic taxonomic approach	305:335	a polyphasic taxonomic approach	305:335	A novel Gram-stain-positive, coccoid or short rod-shaped, moderate-orange-pigmented, halotolerant and psychrotolerant bacterium, designated strain SCU63T, was isolated from a saline soil sample in China, and characterized by a polyphasic taxonomic approach.
30265231	2	58	from	sample	267:272	arg1	China					277:281	China	277:281	China	277:281	A novel Gram-stain-positive, coccoid or short rod-shaped, moderate-orange-pigmented, halotolerant and psychrotolerant bacterium, designated strain SCU63T, was isolated from a saline soil sample in China, and characterized by a polyphasic taxonomic approach.
30265231	4	59	theme	signature	514:522	arg1	nucleotides					524:534	diagnostic signature nucleotides	503:534	Phylogenetic trees as well as diagnostic signature nucleotides in the 16S rRNA gene sequence	473:564	Phylogenetic trees as well as diagnostic signature nucleotides in the 16S rRNA gene sequence supported the view that this strain should be assigned to the genus Planococcus.
30265231	5	60	theme	average	656:662	arg1	identity					675:682	average nucleotide identity	656:682	average nucleotide identity	656:682	Further, average nucleotide identity and digital DNA-DNA hybridization analyses confirmed the separate species status of strain SCU63T relative to the closely related taxa.
30265231	12	61	theme	type	1568:1571	arg1	SCU63T					1583:1588	SCU63T	1583:1588	SCU63T (=CGMCC 1.13628T=KCTC 43001T)	1583:1618	The type strain is SCU63T (=CGMCC 1.13628T=KCTC 43001T).
30265231	12	61	theme	type	1568:1571	arg1	strain					1573:1578	The type strain	1564:1578	The type strain	1564:1578	The type strain is SCU63T (=CGMCC 1.13628T=KCTC 43001T).
30265231	2	62	theme	saline	255:260	arg1	sample					267:272	a saline soil sample	253:272	a saline soil sample in China	253:281	A novel Gram-stain-positive, coccoid or short rod-shaped, moderate-orange-pigmented, halotolerant and psychrotolerant bacterium, designated strain SCU63T, was isolated from a saline soil sample in China, and characterized by a polyphasic taxonomic approach.
30265231	2	63	theme	polyphasic	307:316	arg1	approach					328:335	a polyphasic taxonomic approach	305:335	a polyphasic taxonomic approach	305:335	A novel Gram-stain-positive, coccoid or short rod-shaped, moderate-orange-pigmented, halotolerant and psychrotolerant bacterium, designated strain SCU63T, was isolated from a saline soil sample in China, and characterized by a polyphasic taxonomic approach.
30265231	5	64	theme	nucleotide	664:673	arg1	identity					675:682	average nucleotide identity	656:682	average nucleotide identity	656:682	Further, average nucleotide identity and digital DNA-DNA hybridization analyses confirmed the separate species status of strain SCU63T relative to the closely related taxa.
30265231	10	65	from	species	1465:1471	arg1	Planococcus					1486:1496	the genus Planococcus	1476:1496	the genus Planococcus	1476:1496	Based on the genotypic, phenotypic and chemotaxonomic data, strain SCU63T can be classified as a novel species in the genus Planococcus, for which the name Planococcushalotolerans sp.
30265231	5	66	theme	separate	741:748	arg1	status					758:763	the separate species status	737:763	the separate species status of strain SCU63T relative to the closely related taxa	737:817	Further, average nucleotide identity and digital DNA-DNA hybridization analyses confirmed the separate species status of strain SCU63T relative to the closely related taxa.
30265231	2	67	theme	psychrotolerant	182:196	arg1	bacterium					198:206	A novel Gram-stain-positive, coccoid or short rod-shaped, moderate-orange-pigmented, halotolerant and psychrotolerant bacterium	80:206	A novel Gram-stain-positive, coccoid or short rod-shaped, moderate-orange-pigmented, halotolerant and psychrotolerant bacterium	80:206	A novel Gram-stain-positive, coccoid or short rod-shaped, moderate-orange-pigmented, halotolerant and psychrotolerant bacterium, designated strain SCU63T, was isolated from a saline soil sample in China, and characterized by a polyphasic taxonomic approach.
30265231	7	68	dep	anteiso-C15 	975:986	arg1	alcohol					1003:1009	 1ω7c alcohol	997:1009	 1ω7c alcohol	997:1009	The principal fatty acids were anteiso-C15 : 0, C16 : 1ω7c alcohol, iso-C16 : 0 and iso-C14 : 0, and the dominant isoprenoid quinones were MK-8 and MK-7.
30265231	7	68	dep	anteiso-C15 	975:986	arg1	iso-C14 					1028:1035	iso-C14 	1028:1035	iso-C14 	1028:1035	The principal fatty acids were anteiso-C15 : 0, C16 : 1ω7c alcohol, iso-C16 : 0 and iso-C14 : 0, and the dominant isoprenoid quinones were MK-8 and MK-7.
30265231	7	68	dep	anteiso-C15 	975:986	arg1	 0					1037:1038	 0	1037:1038	 0	1037:1038	The principal fatty acids were anteiso-C15 : 0, C16 : 1ω7c alcohol, iso-C16 : 0 and iso-C14 : 0, and the dominant isoprenoid quinones were MK-8 and MK-7.
30265231	7	68	dep	anteiso-C15 	975:986	arg1	iso-C16 					1012:1019	iso-C16 	1012:1019	iso-C16 	1012:1019	The principal fatty acids were anteiso-C15 : 0, C16 : 1ω7c alcohol, iso-C16 : 0 and iso-C14 : 0, and the dominant isoprenoid quinones were MK-8 and MK-7.
30265231	7	68	dep	anteiso-C15 	975:986	arg1	 0					1021:1022	 0	1021:1022	 0	1021:1022	The principal fatty acids were anteiso-C15 : 0, C16 : 1ω7c alcohol, iso-C16 : 0 and iso-C14 : 0, and the dominant isoprenoid quinones were MK-8 and MK-7.
30265231	7	68	dep	anteiso-C15 	975:986	arg1	C16 					992:995	C16 	992:995	C16 	992:995	The principal fatty acids were anteiso-C15 : 0, C16 : 1ω7c alcohol, iso-C16 : 0 and iso-C14 : 0, and the dominant isoprenoid quinones were MK-8 and MK-7.
30265231	2	69	attach	isolated	239:246	arg2	bacterium					198:206	A novel Gram-stain-positive, coccoid or short rod-shaped, moderate-orange-pigmented, halotolerant and psychrotolerant bacterium	80:206	A novel Gram-stain-positive, coccoid or short rod-shaped, moderate-orange-pigmented, halotolerant and psychrotolerant bacterium	80:206	A novel Gram-stain-positive, coccoid or short rod-shaped, moderate-orange-pigmented, halotolerant and psychrotolerant bacterium, designated strain SCU63T, was isolated from a saline soil sample in China, and characterized by a polyphasic taxonomic approach.
30265231	2	69	attach	isolated	239:246	arg1	sample					267:272	a saline soil sample	253:272	a saline soil sample in China	253:281	A novel Gram-stain-positive, coccoid or short rod-shaped, moderate-orange-pigmented, halotolerant and psychrotolerant bacterium, designated strain SCU63T, was isolated from a saline soil sample in China, and characterized by a polyphasic taxonomic approach.
30265231	5	70	theme	species	750:756	arg1	status					758:763	the separate species status	737:763	the separate species status of strain SCU63T relative to the closely related taxa	737:817	Further, average nucleotide identity and digital DNA-DNA hybridization analyses confirmed the separate species status of strain SCU63T relative to the closely related taxa.
30265231	2	71	theme	coccoid	109:115	arg1	bacterium					198:206	A novel Gram-stain-positive, coccoid or short rod-shaped, moderate-orange-pigmented, halotolerant and psychrotolerant bacterium	80:206	A novel Gram-stain-positive, coccoid or short rod-shaped, moderate-orange-pigmented, halotolerant and psychrotolerant bacterium	80:206	A novel Gram-stain-positive, coccoid or short rod-shaped, moderate-orange-pigmented, halotolerant and psychrotolerant bacterium, designated strain SCU63T, was isolated from a saline soil sample in China, and characterized by a polyphasic taxonomic approach.
30265231	7	72	theme	principal	948:956	arg1	anteiso-C15 					975:986	anteiso-C15 	975:986	anteiso-C15 	975:986	The principal fatty acids were anteiso-C15 : 0, C16 : 1ω7c alcohol, iso-C16 : 0 and iso-C14 : 0, and the dominant isoprenoid quinones were MK-8 and MK-7.
30265231	7	72	theme	principal	948:956	arg1	acids					964:968	The principal fatty acids	944:968	The principal fatty acids	944:968	The principal fatty acids were anteiso-C15 : 0, C16 : 1ω7c alcohol, iso-C16 : 0 and iso-C14 : 0, and the dominant isoprenoid quinones were MK-8 and MK-7.
30265231	10	73	theme	genus	1480:1484	arg1	Planococcus					1486:1496	the genus Planococcus	1476:1496	the genus Planococcus	1476:1496	Based on the genotypic, phenotypic and chemotaxonomic data, strain SCU63T can be classified as a novel species in the genus Planococcus, for which the name Planococcushalotolerans sp.
30265231	7	74	theme	dominant	1049:1056	arg1	quinones					1069:1076	the dominant isoprenoid quinones	1045:1076	the dominant isoprenoid quinones	1045:1076	The principal fatty acids were anteiso-C15 : 0, C16 : 1ω7c alcohol, iso-C16 : 0 and iso-C14 : 0, and the dominant isoprenoid quinones were MK-8 and MK-7.
30265231	7	74	theme	dominant	1049:1056	arg1	MK-8					1083:1086	MK-8	1083:1086	MK-8	1083:1086	The principal fatty acids were anteiso-C15 : 0, C16 : 1ω7c alcohol, iso-C16 : 0 and iso-C14 : 0, and the dominant isoprenoid quinones were MK-8 and MK-7.
30238338	3	0	theme	pale	277:280	arg1	pink					282:285	pale pink	277:285	pale pink in colour	277:295	Cells were observed to be Gram-stain negative, short rod-shaped and colonies to be pale pink in colour.
30238338	5	1	theme	S13-1-2-1T	568:577	arg1	Growth					551:556	Growth	551:556	Growth of strain S13-1-2-1T	551:577	Growth of strain S13-1-2-1T was observed at 10-42 °C, pH 6-8, and in the presence of 0-1.0% NaCl.
30238338	13	2	theme	type	1545:1548	arg1	strain					1550:1555	The type strain	1541:1555	The type strain	1541:1555	The type strain is S13-1-2-1T (= KCTC 33939T = JCM 32248T).
30238338	13	2	theme	type	1545:1548	arg1	S13-1-2-1T					1560:1569	S13-1-2-1T	1560:1569	S13-1-2-1T (= KCTC 33939T = JCM 32248T)	1560:1598	The type strain is S13-1-2-1T (= KCTC 33939T = JCM 32248T).
30238338	4	3	theme	Deinococcus	510:520	arg1	NIO-1023T					532:540	Deinococcus enclensis NIO-1023T	510:540	Deinococcus enclensis NIO-1023T (95.4%)	510:548	Analysis of 16S rRNA gene sequences identified this strain as a member of the genus Deinococcus in the family Deinococcaceae, with high levels of sequence similarity with Deinococcus ficus CC-FR2-10T (97.9%) and Deinococcus enclensis NIO-1023T (95.4%).
30238338	4	3	theme	Deinococcus	510:520	arg1	%					547:547	95.4%	543:547	95.4%	543:547	Analysis of 16S rRNA gene sequences identified this strain as a member of the genus Deinococcus in the family Deinococcaceae, with high levels of sequence similarity with Deinococcus ficus CC-FR2-10T (97.9%) and Deinococcus enclensis NIO-1023T (95.4%).
30238338	4	4	theme	similarity	453:462	arg1	levels					434:439	high levels	429:439	high levels of sequence similarity with Deinococcus ficus CC-FR2-10T (97.9%) and Deinococcus enclensis NIO-1023T (95.4%)	429:548	Analysis of 16S rRNA gene sequences identified this strain as a member of the genus Deinococcus in the family Deinococcaceae, with high levels of sequence similarity with Deinococcus ficus CC-FR2-10T (97.9%) and Deinococcus enclensis NIO-1023T (95.4%).
30238338	6	5	theme	D10	713:715	arg1	KGy					722:724	D10 10.1 KGy	713:724	D10 10.1 KGy	713:724	The isolate was found to exhibit resistance to gamma radiation (D10 10.1 KGy) and UV-light (D10 612 J/m2).
30238338	6	5	theme	D10	713:715	arg1	radiation					702:710	gamma radiation	696:710	gamma radiation (D10 10.1 KGy)	696:725	The isolate was found to exhibit resistance to gamma radiation (D10 10.1 KGy) and UV-light (D10 612 J/m2).
30238338	10	6	theme	DNA-DNA	1227:1233	arg1	hybridization					1235:1247	DNA-DNA hybridization	1227:1247	DNA-DNA hybridization with D. ficus	1227:1261	DNA-DNA hybridization with D. ficus showed a relatedness value of 31.5 ± 4.2%.
30238338	4	7	theme	sequence	444:451	arg1	similarity					453:462	sequence similarity	444:462	sequence similarity with Deinococcus ficus CC-FR2-10T (97.9%) and Deinococcus enclensis NIO-1023T (95.4%)	444:548	Analysis of 16S rRNA gene sequences identified this strain as a member of the genus Deinococcus in the family Deinococcaceae, with high levels of sequence similarity with Deinococcus ficus CC-FR2-10T (97.9%) and Deinococcus enclensis NIO-1023T (95.4%).
30238338	8	8	theme	strain	905:910	arg1	quinone					890:896	The predominant respiratory quinone	862:896	The predominant respiratory quinone of the strain	862:910	The predominant respiratory quinone of the strain was identified as menaquinone-8, the major fatty acids were found to be C16:1ω7c (31.4%), C16:0 (18.4%), and C17:1ω8c (17.4%) and the major polar lipids were observed to be an unidentified phosphoglycolipid and an unidentified glycolipid.
30238338	8	8	theme	strain	905:910	arg1	menaquinone-8					930:942	menaquinone-8	930:942	menaquinone-8	930:942	The predominant respiratory quinone of the strain was identified as menaquinone-8, the major fatty acids were found to be C16:1ω7c (31.4%), C16:0 (18.4%), and C17:1ω8c (17.4%) and the major polar lipids were observed to be an unidentified phosphoglycolipid and an unidentified glycolipid.
30238338	6	9	theme	gamma	696:700	arg1	KGy					722:724	D10 10.1 KGy	713:724	D10 10.1 KGy	713:724	The isolate was found to exhibit resistance to gamma radiation (D10 10.1 KGy) and UV-light (D10 612 J/m2).
30238338	6	9	theme	gamma	696:700	arg1	radiation					702:710	gamma radiation	696:710	gamma radiation (D10 10.1 KGy)	696:725	The isolate was found to exhibit resistance to gamma radiation (D10 10.1 KGy) and UV-light (D10 612 J/m2).
30238338	8	10	theme	unidentified	1126:1137	arg1	glycolipid					1139:1148	an unidentified glycolipid	1123:1148	an unidentified glycolipid	1123:1148	The predominant respiratory quinone of the strain was identified as menaquinone-8, the major fatty acids were found to be C16:1ω7c (31.4%), C16:0 (18.4%), and C17:1ω8c (17.4%) and the major polar lipids were observed to be an unidentified phosphoglycolipid and an unidentified glycolipid.
30238338	13	11	theme	= KCTC	1572:1577	arg1	32248T					1592:1597	= KCTC 33939T = JCM 32248T	1572:1597	= KCTC 33939T = JCM 32248T	1572:1597	The type strain is S13-1-2-1T (= KCTC 33939T = JCM 32248T).
30238338	13	11	theme	= KCTC	1572:1577	arg1	S13-1-2-1T					1560:1569	S13-1-2-1T	1560:1569	S13-1-2-1T (= KCTC 33939T = JCM 32248T)	1560:1598	The type strain is S13-1-2-1T (= KCTC 33939T = JCM 32248T).
30238338	9	12	theme	genomic	1155:1161	arg1	content					1173:1179	The genomic DNA G + C content	1151:1179	The genomic DNA G + C content of the strain	1151:1193	The genomic DNA G + C content of the strain was determined to be 69.2 mol%.
30238338	9	12	theme	genomic	1155:1161	arg1	%					1224:1224	69.2 mol%	1216:1224	69.2 mol%	1216:1224	The genomic DNA G + C content of the strain was determined to be 69.2 mol%.
30238338	7	13	theme	D-glutamic	811:820	arg1	acid					822:825	D-glutamic acid	811:825	D-glutamic acid	811:825	The major peptidoglycan amino acids were identified as D-glutamic acid, glycine, alanine and L-ornithine.
30238338	7	13	theme	D-glutamic	811:820	arg1	acids					786:790	The major peptidoglycan amino acids	756:790	The major peptidoglycan amino acids	756:790	The major peptidoglycan amino acids were identified as D-glutamic acid, glycine, alanine and L-ornithine.
30238338	4	14	dep	Deinococcus	469:479	arg1	ficus					481:485	ficus	481:485	ficus	481:485	Analysis of 16S rRNA gene sequences identified this strain as a member of the genus Deinococcus in the family Deinococcaceae, with high levels of sequence similarity with Deinococcus ficus CC-FR2-10T (97.9%) and Deinococcus enclensis NIO-1023T (95.4%).
30238338	11	15	theme	differentiating	1347:1361	arg1	properties					1374:1383	the differentiating phenotypic properties	1343:1383	the differentiating phenotypic properties	1343:1383	The DNA-DNA hybridization result and the differentiating phenotypic properties clearly indicate that strain S13-1-2-1T represents a novel species in the genus Deinococcus, for which the name Deinococcus terrigena sp.
30238338	5	16	theme	0-1.0	636:640	arg1	%					641:641	%	641:641	%	641:641	Growth of strain S13-1-2-1T was observed at 10-42 °C, pH 6-8, and in the presence of 0-1.0% NaCl.
30238338	11	17	theme	DNA-DNA	1310:1316	arg1	hybridization					1318:1330	The DNA-DNA hybridization result	1306:1337	The DNA-DNA hybridization result	1306:1337	The DNA-DNA hybridization result and the differentiating phenotypic properties clearly indicate that strain S13-1-2-1T represents a novel species in the genus Deinococcus, for which the name Deinococcus terrigena sp.
30238338	9	18	theme	69.2 mol	1216:1223	arg1	content					1173:1179	The genomic DNA G + C content	1151:1179	The genomic DNA G + C content of the strain	1151:1193	The genomic DNA G + C content of the strain was determined to be 69.2 mol%.
30238338	9	18	theme	69.2 mol	1216:1223	arg1	%					1224:1224	69.2 mol%	1216:1224	69.2 mol%	1216:1224	The genomic DNA G + C content of the strain was determined to be 69.2 mol%.
30238338	5	19	theme	%	641:641	arg1	NaCl					643:646	0-1.0% NaCl	636:646	0-1.0% NaCl	636:646	Growth of strain S13-1-2-1T was observed at 10-42 °C, pH 6-8, and in the presence of 0-1.0% NaCl.
30238338	4	20	theme	high	429:432	arg1	levels					434:439	high levels	429:439	high levels of sequence similarity with Deinococcus ficus CC-FR2-10T (97.9%) and Deinococcus enclensis NIO-1023T (95.4%)	429:548	Analysis of 16S rRNA gene sequences identified this strain as a member of the genus Deinococcus in the family Deinococcaceae, with high levels of sequence similarity with Deinococcus ficus CC-FR2-10T (97.9%) and Deinococcus enclensis NIO-1023T (95.4%).
30238338	4	21	theme	gene	319:322	arg1	sequences					324:332	16S rRNA gene sequences	310:332	16S rRNA gene sequences	310:332	Analysis of 16S rRNA gene sequences identified this strain as a member of the genus Deinococcus in the family Deinococcaceae, with high levels of sequence similarity with Deinococcus ficus CC-FR2-10T (97.9%) and Deinococcus enclensis NIO-1023T (95.4%).
30238338	1	22	theme	family	54:59	arg1	Deinococcaceae					61:74	the family Deinococcaceae	50:74	the family Deinococcaceae	50:74	nov., a novel member of the family Deinococcaceae.
30238338	13	23	theme	33939T = JCM	1579:1590	arg1	32248T					1592:1597	= KCTC 33939T = JCM 32248T	1572:1597	= KCTC 33939T = JCM 32248T	1572:1597	The type strain is S13-1-2-1T (= KCTC 33939T = JCM 32248T).
30238338	13	23	theme	33939T = JCM	1579:1590	arg1	S13-1-2-1T					1560:1569	S13-1-2-1T	1560:1569	S13-1-2-1T (= KCTC 33939T = JCM 32248T)	1560:1598	The type strain is S13-1-2-1T (= KCTC 33939T = JCM 32248T).
30238338	0	24	theme	terrigena	12:20	arg1	sp					22:23	Deinococcus terrigena sp	0:23	Deinococcus terrigena sp.	0:24	Deinococcus terrigena sp.
30238338	6	25	theme	D10	741:743	arg1	UV-light					731:738	UV-light	731:738	UV-light (D10 612 J/m2)	731:753	The isolate was found to exhibit resistance to gamma radiation (D10 10.1 KGy) and UV-light (D10 612 J/m2).
30238338	6	25	theme	D10	741:743	arg1	612 J/m2					745:752	D10 612 J/m2	741:752	D10 612 J/m2	741:752	The isolate was found to exhibit resistance to gamma radiation (D10 10.1 KGy) and UV-light (D10 612 J/m2).
30238338	4	26	theme	rRNA	314:317	arg1	sequences					324:332	16S rRNA gene sequences	310:332	16S rRNA gene sequences	310:332	Analysis of 16S rRNA gene sequences identified this strain as a member of the genus Deinococcus in the family Deinococcaceae, with high levels of sequence similarity with Deinococcus ficus CC-FR2-10T (97.9%) and Deinococcus enclensis NIO-1023T (95.4%).
30238338	1	27	theme	Deinococcaceae	61:74	arg1	member					40:45	a novel member	32:45	a novel member of the family Deinococcaceae	32:74	nov., a novel member of the family Deinococcaceae.
30238338	1	27	theme	Deinococcaceae	61:74	arg1	nov.					26:29	nov.	26:29	nov.	26:29	nov., a novel member of the family Deinococcaceae.
30238338	0	28	theme	Deinococcus	0:10	arg1	sp					22:23	Deinococcus terrigena sp	0:23	Deinococcus terrigena sp.	0:24	Deinococcus terrigena sp.
30238338	4	29	from	Deinococcaceae	408:421	arg1	strain					350:355	this strain	345:355	this strain	345:355	Analysis of 16S rRNA gene sequences identified this strain as a member of the genus Deinococcus in the family Deinococcaceae, with high levels of sequence similarity with Deinococcus ficus CC-FR2-10T (97.9%) and Deinococcus enclensis NIO-1023T (95.4%).
30238338	4	29	from	Deinococcaceae	408:421	arg1	member					362:367	a member	360:367	a member of the genus Deinococcus in the family Deinococcaceae	360:421	Analysis of 16S rRNA gene sequences identified this strain as a member of the genus Deinococcus in the family Deinococcaceae, with high levels of sequence similarity with Deinococcus ficus CC-FR2-10T (97.9%) and Deinococcus enclensis NIO-1023T (95.4%).
30238338	8	30	theme	respiratory	878:888	arg1	quinone					890:896	The predominant respiratory quinone	862:896	The predominant respiratory quinone of the strain	862:910	The predominant respiratory quinone of the strain was identified as menaquinone-8, the major fatty acids were found to be C16:1ω7c (31.4%), C16:0 (18.4%), and C17:1ω8c (17.4%) and the major polar lipids were observed to be an unidentified phosphoglycolipid and an unidentified glycolipid.
30238338	8	30	theme	respiratory	878:888	arg1	menaquinone-8					930:942	menaquinone-8	930:942	menaquinone-8	930:942	The predominant respiratory quinone of the strain was identified as menaquinone-8, the major fatty acids were found to be C16:1ω7c (31.4%), C16:0 (18.4%), and C17:1ω8c (17.4%) and the major polar lipids were observed to be an unidentified phosphoglycolipid and an unidentified glycolipid.
30238338	11	31	theme	phenotypic	1363:1372	arg1	properties					1374:1383	the differentiating phenotypic properties	1343:1383	the differentiating phenotypic properties	1343:1383	The DNA-DNA hybridization result and the differentiating phenotypic properties clearly indicate that strain S13-1-2-1T represents a novel species in the genus Deinococcus, for which the name Deinococcus terrigena sp.
30238338	11	32	theme	novel	1438:1442	arg1	species					1444:1450	a novel species	1436:1450	a novel species	1436:1450	The DNA-DNA hybridization result and the differentiating phenotypic properties clearly indicate that strain S13-1-2-1T represents a novel species in the genus Deinococcus, for which the name Deinococcus terrigena sp.
30238338	11	33	theme	Deinococcus	1497:1507	arg1	sp					1519:1520	the name Deinococcus terrigena sp	1488:1520	the name Deinococcus terrigena sp	1488:1520	The DNA-DNA hybridization result and the differentiating phenotypic properties clearly indicate that strain S13-1-2-1T represents a novel species in the genus Deinococcus, for which the name Deinococcus terrigena sp.
30238338	10	34	with	hybridization	1235:1247	arg1	D.					1254:1255	D.	1254:1255	D.	1254:1255	DNA-DNA hybridization with D. ficus showed a relatedness value of 31.5 ± 4.2%.
30238338	4	35	theme	sequences	324:332	arg1	Analysis					298:305	Analysis	298:305	Analysis of 16S rRNA gene sequences	298:332	Analysis of 16S rRNA gene sequences identified this strain as a member of the genus Deinococcus in the family Deinococcaceae, with high levels of sequence similarity with Deinococcus ficus CC-FR2-10T (97.9%) and Deinococcus enclensis NIO-1023T (95.4%).
30238338	9	36	theme	G + C	1167:1171	arg1	content					1173:1179	The genomic DNA G + C content	1151:1179	The genomic DNA G + C content of the strain	1151:1193	The genomic DNA G + C content of the strain was determined to be 69.2 mol%.
30238338	9	36	theme	G + C	1167:1171	arg1	%					1224:1224	69.2 mol%	1216:1224	69.2 mol%	1216:1224	The genomic DNA G + C content of the strain was determined to be 69.2 mol%.
30238338	8	37	theme	major	1046:1050	arg1	phosphoglycolipid					1101:1117	an unidentified phosphoglycolipid	1085:1117	an unidentified phosphoglycolipid	1085:1117	The predominant respiratory quinone of the strain was identified as menaquinone-8, the major fatty acids were found to be C16:1ω7c (31.4%), C16:0 (18.4%), and C17:1ω8c (17.4%) and the major polar lipids were observed to be an unidentified phosphoglycolipid and an unidentified glycolipid.
30238338	8	37	theme	major	1046:1050	arg1	lipids					1058:1063	the major polar lipids	1042:1063	the major polar lipids	1042:1063	The predominant respiratory quinone of the strain was identified as menaquinone-8, the major fatty acids were found to be C16:1ω7c (31.4%), C16:0 (18.4%), and C17:1ω8c (17.4%) and the major polar lipids were observed to be an unidentified phosphoglycolipid and an unidentified glycolipid.
30238338	10	38	dep	D.	1254:1255	arg1	ficus					1257:1261	D. ficus	1254:1261	D. ficus	1254:1261	DNA-DNA hybridization with D. ficus showed a relatedness value of 31.5 ± 4.2%.
30238338	10	39	theme	relatedness	1272:1282	arg1	value					1284:1288	a relatedness value	1270:1288	a relatedness value of 31.5 ± 4.2%	1270:1303	DNA-DNA hybridization with D. ficus showed a relatedness value of 31.5 ± 4.2%.
30238338	11	40	theme	name	1492:1495	arg1	sp					1519:1520	the name Deinococcus terrigena sp	1488:1520	the name Deinococcus terrigena sp	1488:1520	The DNA-DNA hybridization result and the differentiating phenotypic properties clearly indicate that strain S13-1-2-1T represents a novel species in the genus Deinococcus, for which the name Deinococcus terrigena sp.
30238338	8	41	theme	fatty	955:959	arg1	C16:1ω7c					984:991	C16:1ω7c	984:991	C16:1ω7c (31.4%)	984:999	The predominant respiratory quinone of the strain was identified as menaquinone-8, the major fatty acids were found to be C16:1ω7c (31.4%), C16:0 (18.4%), and C17:1ω8c (17.4%) and the major polar lipids were observed to be an unidentified phosphoglycolipid and an unidentified glycolipid.
30238338	8	41	theme	fatty	955:959	arg1	acids					961:965	the major fatty acids	945:965	the major fatty acids	945:965	The predominant respiratory quinone of the strain was identified as menaquinone-8, the major fatty acids were found to be C16:1ω7c (31.4%), C16:0 (18.4%), and C17:1ω8c (17.4%) and the major polar lipids were observed to be an unidentified phosphoglycolipid and an unidentified glycolipid.
30238338	4	42	theme	Deinococcus	382:392	arg1	strain					350:355	this strain	345:355	this strain	345:355	Analysis of 16S rRNA gene sequences identified this strain as a member of the genus Deinococcus in the family Deinococcaceae, with high levels of sequence similarity with Deinococcus ficus CC-FR2-10T (97.9%) and Deinococcus enclensis NIO-1023T (95.4%).
30238338	4	42	theme	Deinococcus	382:392	arg1	member					362:367	a member	360:367	a member of the genus Deinococcus in the family Deinococcaceae	360:421	Analysis of 16S rRNA gene sequences identified this strain as a member of the genus Deinococcus in the family Deinococcaceae, with high levels of sequence similarity with Deinococcus ficus CC-FR2-10T (97.9%) and Deinococcus enclensis NIO-1023T (95.4%).
30238338	7	43	theme	major	760:764	arg1	acid					822:825	D-glutamic acid	811:825	D-glutamic acid	811:825	The major peptidoglycan amino acids were identified as D-glutamic acid, glycine, alanine and L-ornithine.
30238338	7	43	theme	major	760:764	arg1	acids					786:790	The major peptidoglycan amino acids	756:790	The major peptidoglycan amino acids	756:790	The major peptidoglycan amino acids were identified as D-glutamic acid, glycine, alanine and L-ornithine.
30238338	7	43	theme	major	760:764	arg1	L-ornithine					849:859	L-ornithine	849:859	L-ornithine	849:859	The major peptidoglycan amino acids were identified as D-glutamic acid, glycine, alanine and L-ornithine.
30238338	7	43	theme	major	760:764	arg1	alanine					837:843	alanine	837:843	alanine	837:843	The major peptidoglycan amino acids were identified as D-glutamic acid, glycine, alanine and L-ornithine.
30238338	7	43	theme	major	760:764	arg1	glycine					828:834	glycine	828:834	glycine	828:834	The major peptidoglycan amino acids were identified as D-glutamic acid, glycine, alanine and L-ornithine.
30238338	11	44	from	species	1444:1450	arg1	Deinococcus					1465:1475	the genus Deinococcus	1455:1475	the genus Deinococcus	1455:1475	The DNA-DNA hybridization result and the differentiating phenotypic properties clearly indicate that strain S13-1-2-1T represents a novel species in the genus Deinococcus, for which the name Deinococcus terrigena sp.
30238338	2	45	theme	bacterial	79:87	arg1	S13-1-2-1T					97:106	S13-1-2-1T	97:106	S13-1-2-1T	97:106	A bacterial strain, S13-1-2-1T, was isolated from a soil sample collected in Gyeongsangnam-do province, South Korea.
30238338	2	45	theme	bacterial	79:87	arg1	strain					89:94	A bacterial strain	77:94	A bacterial strain	77:94	A bacterial strain, S13-1-2-1T, was isolated from a soil sample collected in Gyeongsangnam-do province, South Korea.
30238338	4	46	from	Deinococcus	382:392	arg1	Deinococcaceae					408:421	the family Deinococcaceae	397:421	the family Deinococcaceae	397:421	Analysis of 16S rRNA gene sequences identified this strain as a member of the genus Deinococcus in the family Deinococcaceae, with high levels of sequence similarity with Deinococcus ficus CC-FR2-10T (97.9%) and Deinococcus enclensis NIO-1023T (95.4%).
30238338	4	47	theme	family	401:406	arg1	Deinococcaceae					408:421	the family Deinococcaceae	397:421	the family Deinococcaceae	397:421	Analysis of 16S rRNA gene sequences identified this strain as a member of the genus Deinococcus in the family Deinococcaceae, with high levels of sequence similarity with Deinococcus ficus CC-FR2-10T (97.9%) and Deinococcus enclensis NIO-1023T (95.4%).
30238338	11	48	dep	Deinococcus	1497:1507	arg1	terrigena					1509:1517	terrigena	1509:1517	terrigena	1509:1517	The DNA-DNA hybridization result and the differentiating phenotypic properties clearly indicate that strain S13-1-2-1T represents a novel species in the genus Deinococcus, for which the name Deinococcus terrigena sp.
30238338	8	49	theme	polar	1052:1056	arg1	phosphoglycolipid					1101:1117	an unidentified phosphoglycolipid	1085:1117	an unidentified phosphoglycolipid	1085:1117	The predominant respiratory quinone of the strain was identified as menaquinone-8, the major fatty acids were found to be C16:1ω7c (31.4%), C16:0 (18.4%), and C17:1ω8c (17.4%) and the major polar lipids were observed to be an unidentified phosphoglycolipid and an unidentified glycolipid.
30238338	8	49	theme	polar	1052:1056	arg1	lipids					1058:1063	the major polar lipids	1042:1063	the major polar lipids	1042:1063	The predominant respiratory quinone of the strain was identified as menaquinone-8, the major fatty acids were found to be C16:1ω7c (31.4%), C16:0 (18.4%), and C17:1ω8c (17.4%) and the major polar lipids were observed to be an unidentified phosphoglycolipid and an unidentified glycolipid.
30238338	4	50	theme	Deinococcus	469:479	arg1	CC-FR2-10T					487:496	Deinococcus ficus CC-FR2-10T	469:496	Deinococcus ficus CC-FR2-10T (97.9%)	469:504	Analysis of 16S rRNA gene sequences identified this strain as a member of the genus Deinococcus in the family Deinococcaceae, with high levels of sequence similarity with Deinococcus ficus CC-FR2-10T (97.9%) and Deinococcus enclensis NIO-1023T (95.4%).
30238338	4	50	theme	Deinococcus	469:479	arg1	%					503:503	97.9%	499:503	97.9%	499:503	Analysis of 16S rRNA gene sequences identified this strain as a member of the genus Deinococcus in the family Deinococcaceae, with high levels of sequence similarity with Deinococcus ficus CC-FR2-10T (97.9%) and Deinococcus enclensis NIO-1023T (95.4%).
30238338	2	51	theme	soil	129:132	arg1	sample					134:139	a soil sample	127:139	a soil sample collected in Gyeongsangnam-do province, South Korea	127:191	A bacterial strain, S13-1-2-1T, was isolated from a soil sample collected in Gyeongsangnam-do province, South Korea.
30238338	11	52	theme	genus	1459:1463	arg1	Deinococcus					1465:1475	the genus Deinococcus	1455:1475	the genus Deinococcus	1455:1475	The DNA-DNA hybridization result and the differentiating phenotypic properties clearly indicate that strain S13-1-2-1T represents a novel species in the genus Deinococcus, for which the name Deinococcus terrigena sp.
30238338	4	53	with	similarity	453:462	arg1	%					503:503	97.9%	499:503	97.9%	499:503	Analysis of 16S rRNA gene sequences identified this strain as a member of the genus Deinococcus in the family Deinococcaceae, with high levels of sequence similarity with Deinococcus ficus CC-FR2-10T (97.9%) and Deinococcus enclensis NIO-1023T (95.4%).
30238338	4	53	with	similarity	453:462	arg1	CC-FR2-10T					487:496	Deinococcus ficus CC-FR2-10T	469:496	Deinococcus ficus CC-FR2-10T (97.9%)	469:504	Analysis of 16S rRNA gene sequences identified this strain as a member of the genus Deinococcus in the family Deinococcaceae, with high levels of sequence similarity with Deinococcus ficus CC-FR2-10T (97.9%) and Deinococcus enclensis NIO-1023T (95.4%).
30238338	4	53	with	similarity	453:462	arg1	NIO-1023T					532:540	Deinococcus enclensis NIO-1023T	510:540	Deinococcus enclensis NIO-1023T (95.4%)	510:548	Analysis of 16S rRNA gene sequences identified this strain as a member of the genus Deinococcus in the family Deinococcaceae, with high levels of sequence similarity with Deinococcus ficus CC-FR2-10T (97.9%) and Deinococcus enclensis NIO-1023T (95.4%).
30238338	4	53	with	similarity	453:462	arg1	%					547:547	95.4%	543:547	95.4%	543:547	Analysis of 16S rRNA gene sequences identified this strain as a member of the genus Deinococcus in the family Deinococcaceae, with high levels of sequence similarity with Deinococcus ficus CC-FR2-10T (97.9%) and Deinococcus enclensis NIO-1023T (95.4%).
30238338	8	54	theme	major	949:953	arg1	C16:1ω7c					984:991	C16:1ω7c	984:991	C16:1ω7c (31.4%)	984:999	The predominant respiratory quinone of the strain was identified as menaquinone-8, the major fatty acids were found to be C16:1ω7c (31.4%), C16:0 (18.4%), and C17:1ω8c (17.4%) and the major polar lipids were observed to be an unidentified phosphoglycolipid and an unidentified glycolipid.
30238338	8	54	theme	major	949:953	arg1	acids					961:965	the major fatty acids	945:965	the major fatty acids	945:965	The predominant respiratory quinone of the strain was identified as menaquinone-8, the major fatty acids were found to be C16:1ω7c (31.4%), C16:0 (18.4%), and C17:1ω8c (17.4%) and the major polar lipids were observed to be an unidentified phosphoglycolipid and an unidentified glycolipid.
30238338	9	55	theme	DNA	1163:1165	arg1	content					1173:1179	The genomic DNA G + C content	1151:1179	The genomic DNA G + C content of the strain	1151:1193	The genomic DNA G + C content of the strain was determined to be 69.2 mol%.
30238338	9	55	theme	DNA	1163:1165	arg1	%					1224:1224	69.2 mol%	1216:1224	69.2 mol%	1216:1224	The genomic DNA G + C content of the strain was determined to be 69.2 mol%.
30238338	10	56	theme	%	1303:1303	arg1	value					1284:1288	a relatedness value	1270:1288	a relatedness value of 31.5 ± 4.2%	1270:1303	DNA-DNA hybridization with D. ficus showed a relatedness value of 31.5 ± 4.2%.
30238338	4	57	theme	16S	310:312	arg1	sequences					324:332	16S rRNA gene sequences	310:332	16S rRNA gene sequences	310:332	Analysis of 16S rRNA gene sequences identified this strain as a member of the genus Deinococcus in the family Deinococcaceae, with high levels of sequence similarity with Deinococcus ficus CC-FR2-10T (97.9%) and Deinococcus enclensis NIO-1023T (95.4%).
30238338	4	58	dep	Deinococcus	510:520	arg1	enclensis					522:530	enclensis	522:530	enclensis	522:530	Analysis of 16S rRNA gene sequences identified this strain as a member of the genus Deinococcus in the family Deinococcaceae, with high levels of sequence similarity with Deinococcus ficus CC-FR2-10T (97.9%) and Deinococcus enclensis NIO-1023T (95.4%).
30238338	7	59	theme	peptidoglycan	766:778	arg1	acid					822:825	D-glutamic acid	811:825	D-glutamic acid	811:825	The major peptidoglycan amino acids were identified as D-glutamic acid, glycine, alanine and L-ornithine.
30238338	7	59	theme	peptidoglycan	766:778	arg1	acids					786:790	The major peptidoglycan amino acids	756:790	The major peptidoglycan amino acids	756:790	The major peptidoglycan amino acids were identified as D-glutamic acid, glycine, alanine and L-ornithine.
30238338	7	59	theme	peptidoglycan	766:778	arg1	L-ornithine					849:859	L-ornithine	849:859	L-ornithine	849:859	The major peptidoglycan amino acids were identified as D-glutamic acid, glycine, alanine and L-ornithine.
30238338	7	59	theme	peptidoglycan	766:778	arg1	alanine					837:843	alanine	837:843	alanine	837:843	The major peptidoglycan amino acids were identified as D-glutamic acid, glycine, alanine and L-ornithine.
30238338	7	59	theme	peptidoglycan	766:778	arg1	glycine					828:834	glycine	828:834	glycine	828:834	The major peptidoglycan amino acids were identified as D-glutamic acid, glycine, alanine and L-ornithine.
30238338	8	60	theme	predominant	866:876	arg1	quinone					890:896	The predominant respiratory quinone	862:896	The predominant respiratory quinone of the strain	862:910	The predominant respiratory quinone of the strain was identified as menaquinone-8, the major fatty acids were found to be C16:1ω7c (31.4%), C16:0 (18.4%), and C17:1ω8c (17.4%) and the major polar lipids were observed to be an unidentified phosphoglycolipid and an unidentified glycolipid.
30238338	8	60	theme	predominant	866:876	arg1	menaquinone-8					930:942	menaquinone-8	930:942	menaquinone-8	930:942	The predominant respiratory quinone of the strain was identified as menaquinone-8, the major fatty acids were found to be C16:1ω7c (31.4%), C16:0 (18.4%), and C17:1ω8c (17.4%) and the major polar lipids were observed to be an unidentified phosphoglycolipid and an unidentified glycolipid.
30238338	3	61	from	pink	282:285	arg1	colour					290:295	colour	290:295	colour	290:295	Cells were observed to be Gram-stain negative, short rod-shaped and colonies to be pale pink in colour.
30238338	7	62	theme	amino	780:784	arg1	acid					822:825	D-glutamic acid	811:825	D-glutamic acid	811:825	The major peptidoglycan amino acids were identified as D-glutamic acid, glycine, alanine and L-ornithine.
30238338	7	62	theme	amino	780:784	arg1	acids					786:790	The major peptidoglycan amino acids	756:790	The major peptidoglycan amino acids	756:790	The major peptidoglycan amino acids were identified as D-glutamic acid, glycine, alanine and L-ornithine.
30238338	7	62	theme	amino	780:784	arg1	L-ornithine					849:859	L-ornithine	849:859	L-ornithine	849:859	The major peptidoglycan amino acids were identified as D-glutamic acid, glycine, alanine and L-ornithine.
30238338	7	62	theme	amino	780:784	arg1	alanine					837:843	alanine	837:843	alanine	837:843	The major peptidoglycan amino acids were identified as D-glutamic acid, glycine, alanine and L-ornithine.
30238338	7	62	theme	amino	780:784	arg1	glycine					828:834	glycine	828:834	glycine	828:834	The major peptidoglycan amino acids were identified as D-glutamic acid, glycine, alanine and L-ornithine.
30238338	4	63	from	member	362:367	arg1	Deinococcaceae					408:421	the family Deinococcaceae	397:421	the family Deinococcaceae	397:421	Analysis of 16S rRNA gene sequences identified this strain as a member of the genus Deinococcus in the family Deinococcaceae, with high levels of sequence similarity with Deinococcus ficus CC-FR2-10T (97.9%) and Deinococcus enclensis NIO-1023T (95.4%).
30238338	4	64	theme	genus	376:380	arg1	Deinococcus					382:392	the genus Deinococcus	372:392	the genus Deinococcus in the family Deinococcaceae	372:421	Analysis of 16S rRNA gene sequences identified this strain as a member of the genus Deinococcus in the family Deinococcaceae, with high levels of sequence similarity with Deinococcus ficus CC-FR2-10T (97.9%) and Deinococcus enclensis NIO-1023T (95.4%).
30238338	2	65	attach	isolated	113:120	arg2	strain					89:94	A bacterial strain	77:94	A bacterial strain	77:94	A bacterial strain, S13-1-2-1T, was isolated from a soil sample collected in Gyeongsangnam-do province, South Korea.
30238338	2	65	attach	isolated	113:120	arg1	sample					134:139	a soil sample	127:139	a soil sample collected in Gyeongsangnam-do province, South Korea	127:191	A bacterial strain, S13-1-2-1T, was isolated from a soil sample collected in Gyeongsangnam-do province, South Korea.
30238338	2	65	attach	isolated	113:120	arg2	S13-1-2-1T					97:106	S13-1-2-1T	97:106	S13-1-2-1T	97:106	A bacterial strain, S13-1-2-1T, was isolated from a soil sample collected in Gyeongsangnam-do province, South Korea.
30238338	11	66	dep	hybridization	1318:1330	arg1	result					1332:1337	result	1332:1337	result	1332:1337	The DNA-DNA hybridization result and the differentiating phenotypic properties clearly indicate that strain S13-1-2-1T represents a novel species in the genus Deinococcus, for which the name Deinococcus terrigena sp.
30238338	5	67	theme	NaCl	643:646	arg1	presence					624:631	the presence	620:631	the presence of 0-1.0% NaCl	620:646	Growth of strain S13-1-2-1T was observed at 10-42 °C, pH 6-8, and in the presence of 0-1.0% NaCl.
30238338	8	68	theme	unidentified	1088:1099	arg1	phosphoglycolipid					1101:1117	an unidentified phosphoglycolipid	1085:1117	an unidentified phosphoglycolipid	1085:1117	The predominant respiratory quinone of the strain was identified as menaquinone-8, the major fatty acids were found to be C16:1ω7c (31.4%), C16:0 (18.4%), and C17:1ω8c (17.4%) and the major polar lipids were observed to be an unidentified phosphoglycolipid and an unidentified glycolipid.
30238338	8	68	theme	unidentified	1088:1099	arg1	lipids					1058:1063	the major polar lipids	1042:1063	the major polar lipids	1042:1063	The predominant respiratory quinone of the strain was identified as menaquinone-8, the major fatty acids were found to be C16:1ω7c (31.4%), C16:0 (18.4%), and C17:1ω8c (17.4%) and the major polar lipids were observed to be an unidentified phosphoglycolipid and an unidentified glycolipid.
30238338	5	69	located	observed	583:590	arg1	pH					605:606	pH 6-8	605:610	pH 6-8	605:610	Growth of strain S13-1-2-1T was observed at 10-42 °C, pH 6-8, and in the presence of 0-1.0% NaCl.
30238338	5	69	located	observed	583:590	arg1	10-42 °C					595:602	10-42 °C	595:602	10-42 °C	595:602	Growth of strain S13-1-2-1T was observed at 10-42 °C, pH 6-8, and in the presence of 0-1.0% NaCl.
30238338	5	69	located	observed	583:590	arg2	Growth					551:556	Growth	551:556	Growth of strain S13-1-2-1T	551:577	Growth of strain S13-1-2-1T was observed at 10-42 °C, pH 6-8, and in the presence of 0-1.0% NaCl.
30238338	1	70	theme	novel	34:38	arg1	member					40:45	a novel member	32:45	a novel member of the family Deinococcaceae	32:74	nov., a novel member of the family Deinococcaceae.
30238338	1	70	theme	novel	34:38	arg1	nov.					26:29	nov.	26:29	nov.	26:29	nov., a novel member of the family Deinococcaceae.
30238338	11	71	theme	strain	1407:1412	arg1	S13-1-2-1T					1414:1423	strain S13-1-2-1T	1407:1423	strain S13-1-2-1T	1407:1423	The DNA-DNA hybridization result and the differentiating phenotypic properties clearly indicate that strain S13-1-2-1T represents a novel species in the genus Deinococcus, for which the name Deinococcus terrigena sp.
30238338	5	72	theme	strain	561:566	arg1	S13-1-2-1T					568:577	strain S13-1-2-1T	561:577	strain S13-1-2-1T	561:577	Growth of strain S13-1-2-1T was observed at 10-42 °C, pH 6-8, and in the presence of 0-1.0% NaCl.
30238338	9	73	theme	strain	1188:1193	arg1	content					1173:1179	The genomic DNA G + C content	1151:1179	The genomic DNA G + C content of the strain	1151:1193	The genomic DNA G + C content of the strain was determined to be 69.2 mol%.
30238338	9	73	theme	strain	1188:1193	arg1	%					1224:1224	69.2 mol%	1216:1224	69.2 mol%	1216:1224	The genomic DNA G + C content of the strain was determined to be 69.2 mol%.
29531080	0	0	theme	immune	80:85	arg1	response					94:101	immune system response	80:101	immune system response	80:101	Chronic stress promotes colitis by disturbing the gut microbiota and triggering immune system response.
29531080	7	1	theme	mucin-2	1026:1032	arg1	expression					1012:1021	stress-induced deficient expression	987:1021	stress-induced deficient expression	987:1021	Furthermore, results showed stress-induced deficient expression of mucin-2 and lysozyme, which may contribute to the disorder of gut microbiota.
29531080	1	2	theme	bowel	152:156	arg1	IBD					167:169	IBD	167:169	IBD	167:169	Chronic stress is known to promote inflammatory bowel disease (IBD), but the underlying mechanism remains largely unresolved.
29531080	1	2	theme	bowel	152:156	arg1	disease					158:164	inflammatory bowel disease	139:164	inflammatory bowel disease (IBD)	139:170	Chronic stress is known to promote inflammatory bowel disease (IBD), but the underlying mechanism remains largely unresolved.
29531080	8	3	theme	stress-induced	1151:1164	arg1	reaction					1173:1180	the stress-induced immune reaction	1147:1180	the stress-induced immune reaction	1147:1180	Of note is that, in the case of cohousing, the stress-induced immune reaction and decreased body weight were abrogated, and transferred gut microbiota from stressed mice to control mice was sufficient to facilitate DSS-induced colitis.
29531080	3	4	theme	MLN-associated	631:644	arg1	cells					653:657	MLN-associated immune cells	631:657	MLN-associated immune cells	631:657	Circulating total white blood cells were significantly increased after stress, and the proportion of MLN-associated immune cells were largely changed.
29531080	2	5	theme	proinflammatory	385:399	arg1	macrophages					408:418	proinflammatory ly6Chi macrophages	385:418	proinflammatory ly6Chi macrophages	385:418	Here, we found chronic stress to sensitize mice to dextran sulfate sodium (DSS)-induced colitis; to increase the infiltration of B cells, neutrophils, and proinflammatory ly6Chi macrophages in colonic lamina propria; and to present with decreased thymus and mesenteric lymph node (MLN) coefficients.
29531080	8	6	from	mice	1269:1272	arg1	microbiota					1244:1253	transferred gut microbiota	1228:1253	transferred gut microbiota from stressed mice to control mice	1228:1288	Of note is that, in the case of cohousing, the stress-induced immune reaction and decreased body weight were abrogated, and transferred gut microbiota from stressed mice to control mice was sufficient to facilitate DSS-induced colitis.
29531080	2	7	theme	-induced	309:316	arg1	colitis					318:324	dextran sulfate sodium (DSS)-induced colitis	281:324	dextran sulfate sodium (DSS)-induced colitis	281:324	Here, we found chronic stress to sensitize mice to dextran sulfate sodium (DSS)-induced colitis; to increase the infiltration of B cells, neutrophils, and proinflammatory ly6Chi macrophages in colonic lamina propria; and to present with decreased thymus and mesenteric lymph node (MLN) coefficients.
29531080	2	8	theme	thymus	477:482	arg1	coefficients					516:527	decreased thymus and mesenteric lymph node (MLN) coefficients	467:527	decreased thymus and mesenteric lymph node (MLN) coefficients	467:527	Here, we found chronic stress to sensitize mice to dextran sulfate sodium (DSS)-induced colitis; to increase the infiltration of B cells, neutrophils, and proinflammatory ly6Chi macrophages in colonic lamina propria; and to present with decreased thymus and mesenteric lymph node (MLN) coefficients.
29531080	3	9	theme	immune	646:651	arg1	cells					653:657	MLN-associated immune cells	631:657	MLN-associated immune cells	631:657	Circulating total white blood cells were significantly increased after stress, and the proportion of MLN-associated immune cells were largely changed.
29531080	7	10	theme	gut	1088:1090	arg1	microbiota					1092:1101	gut microbiota	1088:1101	gut microbiota	1088:1101	Furthermore, results showed stress-induced deficient expression of mucin-2 and lysozyme, which may contribute to the disorder of gut microbiota.
29531080	4	11	theme	IL-6/STAT3	719:728	arg1	signaling					730:738	IL-6/STAT3 signaling	719:738	IL-6/STAT3 signaling	719:738	Results showed a marked activation of IL-6/STAT3 signaling by stress.
29531080	4	12	theme	marked	698:703	arg1	activation					705:714	a marked activation	696:714	a marked activation of IL-6/STAT3 signaling by stress	696:748	Results showed a marked activation of IL-6/STAT3 signaling by stress.
29531080	0	13	theme	system	87:92	arg1	response					94:101	immune system response	80:101	immune system response	80:101	Chronic stress promotes colitis by disturbing the gut microbiota and triggering immune system response.
29531080	6	14	theme	gut	854:856	arg1	microbiota					858:867	gut microbiota	854:867	gut microbiota	854:867	Interestingly, the composition of gut microbiota was dramatically changed after stress, with expansion of inflammation-promoting bacteria.
29531080	7	15	theme	microbiota	1092:1101	arg1	disorder					1076:1083	the disorder	1072:1083	the disorder of gut microbiota	1072:1101	Furthermore, results showed stress-induced deficient expression of mucin-2 and lysozyme, which may contribute to the disorder of gut microbiota.
29531080	9	16	theme	gut	1362:1364	arg1	microbiota					1366:1375	gut microbiota	1362:1375	gut microbiota	1362:1375	The important role of gut microbiota was further reinforced by broad-spectrum antibiotic treatment.
29531080	8	17	theme	DSS-induced	1319:1329	arg1	colitis					1331:1337	DSS-induced colitis	1319:1337	DSS-induced colitis	1319:1337	Of note is that, in the case of cohousing, the stress-induced immune reaction and decreased body weight were abrogated, and transferred gut microbiota from stressed mice to control mice was sufficient to facilitate DSS-induced colitis.
29531080	9	18	theme	broad-spectrum	1403:1416	arg1	treatment					1429:1437	broad-spectrum antibiotic treatment	1403:1437	broad-spectrum antibiotic treatment	1403:1437	The important role of gut microbiota was further reinforced by broad-spectrum antibiotic treatment.
29531080	5	19	theme	detrimental	755:765	arg1	action					767:772	The detrimental action	751:772	The detrimental action of stress	751:782	The detrimental action of stress was not terminated in IL-6-/- mice.
29531080	2	20	theme	chronic	245:251	arg1	stress					253:258	chronic stress	245:258	chronic stress to sensitize mice to dextran sulfate sodium (DSS)-induced colitis; to increase the infiltration of B cells, neutrophils, and proinflammatory ly6Chi macrophages in colonic lamina propria; and to present with decreased thymus and mesenteric lymph node (MLN) coefficients	245:527	Here, we found chronic stress to sensitize mice to dextran sulfate sodium (DSS)-induced colitis; to increase the infiltration of B cells, neutrophils, and proinflammatory ly6Chi macrophages in colonic lamina propria; and to present with decreased thymus and mesenteric lymph node (MLN) coefficients.
29531080	8	21	theme	body	1196:1199	arg1	weight					1201:1206	decreased body weight	1186:1206	decreased body weight	1186:1206	Of note is that, in the case of cohousing, the stress-induced immune reaction and decreased body weight were abrogated, and transferred gut microbiota from stressed mice to control mice was sufficient to facilitate DSS-induced colitis.
29531080	2	22	theme	ly6Chi	401:406	arg1	macrophages					408:418	proinflammatory ly6Chi macrophages	385:418	proinflammatory ly6Chi macrophages	385:418	Here, we found chronic stress to sensitize mice to dextran sulfate sodium (DSS)-induced colitis; to increase the infiltration of B cells, neutrophils, and proinflammatory ly6Chi macrophages in colonic lamina propria; and to present with decreased thymus and mesenteric lymph node (MLN) coefficients.
29531080	10	23	dep	disturbs	1495:1502	arg1	facilitating					1558:1569	facilitating	1558:1569	facilitating DSS-induced colitis	1558:1589	Taken together, our results reveal that chronic stress disturbs gut microbiota, triggering immune system response and facilitating DSS-induced colitis.
29531080	10	23	dep	disturbs	1495:1502	arg1	triggering					1520:1529	triggering	1520:1529	triggering immune system response	1520:1552	Taken together, our results reveal that chronic stress disturbs gut microbiota, triggering immune system response and facilitating DSS-induced colitis.
29531080	10	24	theme	gut	1504:1506	arg1	microbiota					1508:1517	gut microbiota	1504:1517	gut microbiota	1504:1517	Taken together, our results reveal that chronic stress disturbs gut microbiota, triggering immune system response and facilitating DSS-induced colitis.
29531080	0	25	theme	Chronic	0:6	arg1	stress					8:13	Chronic stress	0:13	Chronic stress	0:13	Chronic stress promotes colitis by disturbing the gut microbiota and triggering immune system response.
29531080	6	26	theme	bacteria	949:956	arg1	expansion					913:921	expansion	913:921	expansion of inflammation-promoting bacteria	913:956	Interestingly, the composition of gut microbiota was dramatically changed after stress, with expansion of inflammation-promoting bacteria.
29531080	10	27	theme	chronic	1480:1486	arg1	stress					1488:1493	chronic stress	1480:1493	chronic stress	1480:1493	Taken together, our results reveal that chronic stress disturbs gut microbiota, triggering immune system response and facilitating DSS-induced colitis.
29531080	5	28	theme	stress	777:782	arg1	action					767:772	The detrimental action	751:772	The detrimental action of stress	751:782	The detrimental action of stress was not terminated in IL-6-/- mice.
29531080	6	29	theme	inflammation-promoting	926:947	arg1	bacteria					949:956	inflammation-promoting bacteria	926:956	inflammation-promoting bacteria	926:956	Interestingly, the composition of gut microbiota was dramatically changed after stress, with expansion of inflammation-promoting bacteria.
29531080	2	30	theme	node	505:508	arg1	coefficients					516:527	decreased thymus and mesenteric lymph node (MLN) coefficients	467:527	decreased thymus and mesenteric lymph node (MLN) coefficients	467:527	Here, we found chronic stress to sensitize mice to dextran sulfate sodium (DSS)-induced colitis; to increase the infiltration of B cells, neutrophils, and proinflammatory ly6Chi macrophages in colonic lamina propria; and to present with decreased thymus and mesenteric lymph node (MLN) coefficients.
29531080	2	31	theme	neutrophils	368:378	arg1	infiltration					343:354	the infiltration	339:354	the infiltration of B cells, neutrophils, and proinflammatory ly6Chi macrophages in colonic lamina propria	339:444	Here, we found chronic stress to sensitize mice to dextran sulfate sodium (DSS)-induced colitis; to increase the infiltration of B cells, neutrophils, and proinflammatory ly6Chi macrophages in colonic lamina propria; and to present with decreased thymus and mesenteric lymph node (MLN) coefficients.
29531080	2	32	theme	lymph	499:503	arg1	MLN					511:513	MLN	511:513	MLN	511:513	Here, we found chronic stress to sensitize mice to dextran sulfate sodium (DSS)-induced colitis; to increase the infiltration of B cells, neutrophils, and proinflammatory ly6Chi macrophages in colonic lamina propria; and to present with decreased thymus and mesenteric lymph node (MLN) coefficients.
29531080	2	32	theme	lymph	499:503	arg1	node					505:508	mesenteric lymph node	488:508	mesenteric lymph node (MLN)	488:514	Here, we found chronic stress to sensitize mice to dextran sulfate sodium (DSS)-induced colitis; to increase the infiltration of B cells, neutrophils, and proinflammatory ly6Chi macrophages in colonic lamina propria; and to present with decreased thymus and mesenteric lymph node (MLN) coefficients.
29531080	7	33	theme	stress-induced	987:1000	arg1	expression					1012:1021	stress-induced deficient expression	987:1021	stress-induced deficient expression	987:1021	Furthermore, results showed stress-induced deficient expression of mucin-2 and lysozyme, which may contribute to the disorder of gut microbiota.
29531080	1	34	theme	Chronic	104:110	arg1	stress					112:117	Chronic stress	104:117	Chronic stress	104:117	Chronic stress is known to promote inflammatory bowel disease (IBD), but the underlying mechanism remains largely unresolved.
29531080	8	35	theme	control	1277:1283	arg1	mice					1285:1288	control mice	1277:1288	control mice	1277:1288	Of note is that, in the case of cohousing, the stress-induced immune reaction and decreased body weight were abrogated, and transferred gut microbiota from stressed mice to control mice was sufficient to facilitate DSS-induced colitis.
29531080	2	36	theme	lamina	431:436	arg1	propria					438:444	colonic lamina propria	423:444	colonic lamina propria	423:444	Here, we found chronic stress to sensitize mice to dextran sulfate sodium (DSS)-induced colitis; to increase the infiltration of B cells, neutrophils, and proinflammatory ly6Chi macrophages in colonic lamina propria; and to present with decreased thymus and mesenteric lymph node (MLN) coefficients.
29531080	2	37	theme	mesenteric	488:497	arg1	MLN					511:513	MLN	511:513	MLN	511:513	Here, we found chronic stress to sensitize mice to dextran sulfate sodium (DSS)-induced colitis; to increase the infiltration of B cells, neutrophils, and proinflammatory ly6Chi macrophages in colonic lamina propria; and to present with decreased thymus and mesenteric lymph node (MLN) coefficients.
29531080	2	37	theme	mesenteric	488:497	arg1	node					505:508	mesenteric lymph node	488:508	mesenteric lymph node (MLN)	488:514	Here, we found chronic stress to sensitize mice to dextran sulfate sodium (DSS)-induced colitis; to increase the infiltration of B cells, neutrophils, and proinflammatory ly6Chi macrophages in colonic lamina propria; and to present with decreased thymus and mesenteric lymph node (MLN) coefficients.
29531080	1	38	theme	underlying	181:190	arg1	mechanism					192:200	the underlying mechanism	177:200	the underlying mechanism	177:200	Chronic stress is known to promote inflammatory bowel disease (IBD), but the underlying mechanism remains largely unresolved.
29531080	3	39	theme	total	542:546	arg1	cells					560:564	Circulating total white blood cells	530:564	Circulating total white blood cells	530:564	Circulating total white blood cells were significantly increased after stress, and the proportion of MLN-associated immune cells were largely changed.
29531080	2	40	theme	cells	361:365	arg1	infiltration					343:354	the infiltration	339:354	the infiltration of B cells, neutrophils, and proinflammatory ly6Chi macrophages in colonic lamina propria	339:444	Here, we found chronic stress to sensitize mice to dextran sulfate sodium (DSS)-induced colitis; to increase the infiltration of B cells, neutrophils, and proinflammatory ly6Chi macrophages in colonic lamina propria; and to present with decreased thymus and mesenteric lymph node (MLN) coefficients.
29531080	2	41	theme	colonic	423:429	arg1	propria					438:444	colonic lamina propria	423:444	colonic lamina propria	423:444	Here, we found chronic stress to sensitize mice to dextran sulfate sodium (DSS)-induced colitis; to increase the infiltration of B cells, neutrophils, and proinflammatory ly6Chi macrophages in colonic lamina propria; and to present with decreased thymus and mesenteric lymph node (MLN) coefficients.
29531080	8	42	theme	immune	1166:1171	arg1	reaction					1173:1180	the stress-induced immune reaction	1147:1180	the stress-induced immune reaction	1147:1180	Of note is that, in the case of cohousing, the stress-induced immune reaction and decreased body weight were abrogated, and transferred gut microbiota from stressed mice to control mice was sufficient to facilitate DSS-induced colitis.
29531080	8	43	theme	gut	1240:1242	arg1	microbiota					1244:1253	transferred gut microbiota	1228:1253	transferred gut microbiota from stressed mice to control mice	1228:1288	Of note is that, in the case of cohousing, the stress-induced immune reaction and decreased body weight were abrogated, and transferred gut microbiota from stressed mice to control mice was sufficient to facilitate DSS-induced colitis.
29531080	3	44	theme	white	548:552	arg1	cells					560:564	Circulating total white blood cells	530:564	Circulating total white blood cells	530:564	Circulating total white blood cells were significantly increased after stress, and the proportion of MLN-associated immune cells were largely changed.
29531080	7	45	theme	deficient	1002:1010	arg1	expression					1012:1021	stress-induced deficient expression	987:1021	stress-induced deficient expression	987:1021	Furthermore, results showed stress-induced deficient expression of mucin-2 and lysozyme, which may contribute to the disorder of gut microbiota.
29531080	2	46	theme	B	359:359	arg1	cells					361:365	B cells	359:365	B cells	359:365	Here, we found chronic stress to sensitize mice to dextran sulfate sodium (DSS)-induced colitis; to increase the infiltration of B cells, neutrophils, and proinflammatory ly6Chi macrophages in colonic lamina propria; and to present with decreased thymus and mesenteric lymph node (MLN) coefficients.
29531080	0	47	theme	gut	50:52	arg1	microbiota					54:63	the gut microbiota	46:63	the gut microbiota	46:63	Chronic stress promotes colitis by disturbing the gut microbiota and triggering immune system response.
29531080	10	48	theme	DSS-induced	1571:1581	arg1	colitis					1583:1589	DSS-induced colitis	1571:1589	DSS-induced colitis	1571:1589	Taken together, our results reveal that chronic stress disturbs gut microbiota, triggering immune system response and facilitating DSS-induced colitis.
29531080	10	49	theme	immune	1531:1536	arg1	response					1545:1552	immune system response	1531:1552	immune system response	1531:1552	Taken together, our results reveal that chronic stress disturbs gut microbiota, triggering immune system response and facilitating DSS-induced colitis.
29531080	2	50	theme	macrophages	408:418	arg1	infiltration					343:354	the infiltration	339:354	the infiltration of B cells, neutrophils, and proinflammatory ly6Chi macrophages in colonic lamina propria	339:444	Here, we found chronic stress to sensitize mice to dextran sulfate sodium (DSS)-induced colitis; to increase the infiltration of B cells, neutrophils, and proinflammatory ly6Chi macrophages in colonic lamina propria; and to present with decreased thymus and mesenteric lymph node (MLN) coefficients.
29531080	7	51	theme	lysozyme	1038:1045	arg1	expression					1012:1021	stress-induced deficient expression	987:1021	stress-induced deficient expression	987:1021	Furthermore, results showed stress-induced deficient expression of mucin-2 and lysozyme, which may contribute to the disorder of gut microbiota.
29531080	3	52	theme	Circulating	530:540	arg1	cells					560:564	Circulating total white blood cells	530:564	Circulating total white blood cells	530:564	Circulating total white blood cells were significantly increased after stress, and the proportion of MLN-associated immune cells were largely changed.
29531080	9	53	theme	important	1344:1352	arg1	role					1354:1357	The important role	1340:1357	The important role of gut microbiota	1340:1375	The important role of gut microbiota was further reinforced by broad-spectrum antibiotic treatment.
29531080	2	54	from	infiltration	343:354	arg1	propria					438:444	colonic lamina propria	423:444	colonic lamina propria	423:444	Here, we found chronic stress to sensitize mice to dextran sulfate sodium (DSS)-induced colitis; to increase the infiltration of B cells, neutrophils, and proinflammatory ly6Chi macrophages in colonic lamina propria; and to present with decreased thymus and mesenteric lymph node (MLN) coefficients.
29531080	8	55	theme	transferred	1228:1238	arg1	microbiota					1244:1253	transferred gut microbiota	1228:1253	transferred gut microbiota from stressed mice to control mice	1228:1288	Of note is that, in the case of cohousing, the stress-induced immune reaction and decreased body weight were abrogated, and transferred gut microbiota from stressed mice to control mice was sufficient to facilitate DSS-induced colitis.
29531080	4	56	theme	signaling	730:738	arg1	activation					705:714	a marked activation	696:714	a marked activation of IL-6/STAT3 signaling by stress	696:748	Results showed a marked activation of IL-6/STAT3 signaling by stress.
29531080	8	57	theme	cohousing	1136:1144	arg1	case					1128:1131	the case	1124:1131	the case of cohousing	1124:1144	Of note is that, in the case of cohousing, the stress-induced immune reaction and decreased body weight were abrogated, and transferred gut microbiota from stressed mice to control mice was sufficient to facilitate DSS-induced colitis.
29531080	8	58	theme	stressed	1260:1267	arg1	mice					1269:1272	stressed mice	1260:1272	stressed mice	1260:1272	Of note is that, in the case of cohousing, the stress-induced immune reaction and decreased body weight were abrogated, and transferred gut microbiota from stressed mice to control mice was sufficient to facilitate DSS-induced colitis.
29531080	10	59	theme	system	1538:1543	arg1	response					1545:1552	immune system response	1531:1552	immune system response	1531:1552	Taken together, our results reveal that chronic stress disturbs gut microbiota, triggering immune system response and facilitating DSS-induced colitis.
29531080	5	60	theme	IL-6-/-	806:812	arg1	mice					814:817	IL-6-/- mice	806:817	IL-6-/- mice	806:817	The detrimental action of stress was not terminated in IL-6-/- mice.
29531080	3	61	theme	blood	554:558	arg1	cells					560:564	Circulating total white blood cells	530:564	Circulating total white blood cells	530:564	Circulating total white blood cells were significantly increased after stress, and the proportion of MLN-associated immune cells were largely changed.
29531080	2	62	dep	stress	253:258	arg1	increase					330:337	increase	330:337	to increase the infiltration of B cells, neutrophils, and proinflammatory ly6Chi macrophages in colonic lamina propria	327:444	Here, we found chronic stress to sensitize mice to dextran sulfate sodium (DSS)-induced colitis; to increase the infiltration of B cells, neutrophils, and proinflammatory ly6Chi macrophages in colonic lamina propria; and to present with decreased thymus and mesenteric lymph node (MLN) coefficients.
29531080	2	62	dep	stress	253:258	arg1	sensitize					263:271	sensitize	263:271	to sensitize mice to dextran sulfate sodium (DSS)-induced colitis	260:324	Here, we found chronic stress to sensitize mice to dextran sulfate sodium (DSS)-induced colitis; to increase the infiltration of B cells, neutrophils, and proinflammatory ly6Chi macrophages in colonic lamina propria; and to present with decreased thymus and mesenteric lymph node (MLN) coefficients.
29531080	2	62	dep	stress	253:258	arg1	present					454:460	present	454:460	to present with decreased thymus and mesenteric lymph node (MLN) coefficients	451:527	Here, we found chronic stress to sensitize mice to dextran sulfate sodium (DSS)-induced colitis; to increase the infiltration of B cells, neutrophils, and proinflammatory ly6Chi macrophages in colonic lamina propria; and to present with decreased thymus and mesenteric lymph node (MLN) coefficients.
29531080	9	63	theme	antibiotic	1418:1427	arg1	treatment					1429:1437	broad-spectrum antibiotic treatment	1403:1437	broad-spectrum antibiotic treatment	1403:1437	The important role of gut microbiota was further reinforced by broad-spectrum antibiotic treatment.
29531080	6	64	theme	microbiota	858:867	arg1	composition					839:849	the composition	835:849	the composition of gut microbiota	835:867	Interestingly, the composition of gut microbiota was dramatically changed after stress, with expansion of inflammation-promoting bacteria.
29531080	8	65	theme	decreased	1186:1194	arg1	weight					1201:1206	decreased body weight	1186:1206	decreased body weight	1186:1206	Of note is that, in the case of cohousing, the stress-induced immune reaction and decreased body weight were abrogated, and transferred gut microbiota from stressed mice to control mice was sufficient to facilitate DSS-induced colitis.
29531080	3	66	theme	cells	653:657	arg1	proportion					617:626	the proportion	613:626	the proportion of MLN-associated immune cells	613:657	Circulating total white blood cells were significantly increased after stress, and the proportion of MLN-associated immune cells were largely changed.
29531080	9	67	theme	microbiota	1366:1375	arg1	role					1354:1357	The important role	1340:1357	The important role of gut microbiota	1340:1375	The important role of gut microbiota was further reinforced by broad-spectrum antibiotic treatment.
29531080	1	68	theme	inflammatory	139:150	arg1	IBD					167:169	IBD	167:169	IBD	167:169	Chronic stress is known to promote inflammatory bowel disease (IBD), but the underlying mechanism remains largely unresolved.
29531080	1	68	theme	inflammatory	139:150	arg1	disease					158:164	inflammatory bowel disease	139:164	inflammatory bowel disease (IBD)	139:170	Chronic stress is known to promote inflammatory bowel disease (IBD), but the underlying mechanism remains largely unresolved.
29531080	2	69	theme	decreased	467:475	arg1	coefficients					516:527	decreased thymus and mesenteric lymph node (MLN) coefficients	467:527	decreased thymus and mesenteric lymph node (MLN) coefficients	467:527	Here, we found chronic stress to sensitize mice to dextran sulfate sodium (DSS)-induced colitis; to increase the infiltration of B cells, neutrophils, and proinflammatory ly6Chi macrophages in colonic lamina propria; and to present with decreased thymus and mesenteric lymph node (MLN) coefficients.
31134988	2	0	theme	high	492:495	arg1	hydrophilicity					497:510	its high hydrophilicity	488:510	its high hydrophilicity	488:510	Among them, cysteine-MOF was found to be a multifunctional composite as an excellent matrix and an efficient adsorbent for N-glycans due to its high hydrophilicity, low background interference and unique size-exclusion effect.
31134988	2	0	theme	high	492:495	arg1	effect					567:572	unique size-exclusion effect	545:572	unique size-exclusion effect	545:572	Among them, cysteine-MOF was found to be a multifunctional composite as an excellent matrix and an efficient adsorbent for N-glycans due to its high hydrophilicity, low background interference and unique size-exclusion effect.
31134988	2	0	theme	high	492:495	arg1	interference					528:539	low background interference	513:539	low background interference	513:539	Among them, cysteine-MOF was found to be a multifunctional composite as an excellent matrix and an efficient adsorbent for N-glycans due to its high hydrophilicity, low background interference and unique size-exclusion effect.
31134988	1	1	theme	functional	165:174	arg1	MOFs					202:205	MOFs	202:205	MOFs	202:205	A variety of functional metal-organic frameworks (MOFs) were prepared via a universal method and served as matrices in small molecule analysis using laser desorption/ionization mass spectrometry.
31134988	1	1	theme	functional	165:174	arg1	frameworks					190:199	functional metal-organic frameworks	165:199	functional metal-organic frameworks (MOFs)	165:206	A variety of functional metal-organic frameworks (MOFs) were prepared via a universal method and served as matrices in small molecule analysis using laser desorption/ionization mass spectrometry.
31134988	0	2	theme	molecules	89:97	arg1	analysis					71:78	analysis	71:78	analysis of small molecules using laser desorption/ionization mass spectrometry	71:149	A flexible and multifunctional metal-organic framework as a matrix for analysis of small molecules using laser desorption/ionization mass spectrometry.
31134988	0	3	theme	small	83:87	arg1	molecules					89:97	small molecules	83:97	small molecules using laser desorption/ionization mass spectrometry	83:149	A flexible and multifunctional metal-organic framework as a matrix for analysis of small molecules using laser desorption/ionization mass spectrometry.
31134988	2	4	theme	background	517:526	arg1	hydrophilicity					497:510	its high hydrophilicity	488:510	its high hydrophilicity	488:510	Among them, cysteine-MOF was found to be a multifunctional composite as an excellent matrix and an efficient adsorbent for N-glycans due to its high hydrophilicity, low background interference and unique size-exclusion effect.
31134988	2	4	theme	background	517:526	arg1	interference					528:539	low background interference	513:539	low background interference	513:539	Among them, cysteine-MOF was found to be a multifunctional composite as an excellent matrix and an efficient adsorbent for N-glycans due to its high hydrophilicity, low background interference and unique size-exclusion effect.
31134988	1	5	theme	metal-organic	176:188	arg1	MOFs					202:205	MOFs	202:205	MOFs	202:205	A variety of functional metal-organic frameworks (MOFs) were prepared via a universal method and served as matrices in small molecule analysis using laser desorption/ionization mass spectrometry.
31134988	1	5	theme	metal-organic	176:188	arg1	frameworks					190:199	functional metal-organic frameworks	165:199	functional metal-organic frameworks (MOFs)	165:206	A variety of functional metal-organic frameworks (MOFs) were prepared via a universal method and served as matrices in small molecule analysis using laser desorption/ionization mass spectrometry.
31134988	2	6	theme	low	513:515	arg1	hydrophilicity					497:510	its high hydrophilicity	488:510	its high hydrophilicity	488:510	Among them, cysteine-MOF was found to be a multifunctional composite as an excellent matrix and an efficient adsorbent for N-glycans due to its high hydrophilicity, low background interference and unique size-exclusion effect.
31134988	2	6	theme	low	513:515	arg1	interference					528:539	low background interference	513:539	low background interference	513:539	Among them, cysteine-MOF was found to be a multifunctional composite as an excellent matrix and an efficient adsorbent for N-glycans due to its high hydrophilicity, low background interference and unique size-exclusion effect.
31134988	2	7	theme	multifunctional	391:405	arg1	composite					407:415	a multifunctional composite	389:415	a multifunctional composite	389:415	Among them, cysteine-MOF was found to be a multifunctional composite as an excellent matrix and an efficient adsorbent for N-glycans due to its high hydrophilicity, low background interference and unique size-exclusion effect.
31134988	2	7	theme	multifunctional	391:405	arg1	cysteine-MOF					360:371	cysteine-MOF	360:371	cysteine-MOF	360:371	Among them, cysteine-MOF was found to be a multifunctional composite as an excellent matrix and an efficient adsorbent for N-glycans due to its high hydrophilicity, low background interference and unique size-exclusion effect.
31134988	0	8	theme	flexible	2:9	arg1	framework					45:53	A flexible and multifunctional metal-organic framework	0:53	A flexible and multifunctional metal-organic framework as a matrix for analysis of small molecules using laser desorption/ionization mass spectrometry.	0:150	A flexible and multifunctional metal-organic framework as a matrix for analysis of small molecules using laser desorption/ionization mass spectrometry.
31134988	1	9	theme	small	271:275	arg1	analysis					286:293	small molecule analysis	271:293	small molecule analysis using laser desorption/ionization mass spectrometry	271:345	A variety of functional metal-organic frameworks (MOFs) were prepared via a universal method and served as matrices in small molecule analysis using laser desorption/ionization mass spectrometry.
31134988	0	10	theme	multifunctional	15:29	arg1	framework					45:53	A flexible and multifunctional metal-organic framework	0:53	A flexible and multifunctional metal-organic framework as a matrix for analysis of small molecules using laser desorption/ionization mass spectrometry.	0:150	A flexible and multifunctional metal-organic framework as a matrix for analysis of small molecules using laser desorption/ionization mass spectrometry.
31134988	1	11	theme	molecule	277:284	arg1	analysis					286:293	small molecule analysis	271:293	small molecule analysis using laser desorption/ionization mass spectrometry	271:345	A variety of functional metal-organic frameworks (MOFs) were prepared via a universal method and served as matrices in small molecule analysis using laser desorption/ionization mass spectrometry.
31134988	0	12	theme	mass	133:136	arg1	spectrometry					138:149	laser desorption/ionization mass spectrometry	105:149	laser desorption/ionization mass spectrometry	105:149	A flexible and multifunctional metal-organic framework as a matrix for analysis of small molecules using laser desorption/ionization mass spectrometry.
31134988	0	13	theme	desorption/ionization	111:131	arg1	spectrometry					138:149	laser desorption/ionization mass spectrometry	105:149	laser desorption/ionization mass spectrometry	105:149	A flexible and multifunctional metal-organic framework as a matrix for analysis of small molecules using laser desorption/ionization mass spectrometry.
31134988	2	14	theme	excellent	423:431	arg1	matrix					433:438	an excellent matrix	420:438	an excellent matrix	420:438	Among them, cysteine-MOF was found to be a multifunctional composite as an excellent matrix and an efficient adsorbent for N-glycans due to its high hydrophilicity, low background interference and unique size-exclusion effect.
31134988	2	15	theme	size-exclusion	552:565	arg1	effect					567:572	unique size-exclusion effect	545:572	unique size-exclusion effect	545:572	Among them, cysteine-MOF was found to be a multifunctional composite as an excellent matrix and an efficient adsorbent for N-glycans due to its high hydrophilicity, low background interference and unique size-exclusion effect.
31134988	2	15	theme	size-exclusion	552:565	arg1	hydrophilicity					497:510	its high hydrophilicity	488:510	its high hydrophilicity	488:510	Among them, cysteine-MOF was found to be a multifunctional composite as an excellent matrix and an efficient adsorbent for N-glycans due to its high hydrophilicity, low background interference and unique size-exclusion effect.
31134988	0	16	theme	laser	105:109	arg1	spectrometry					138:149	laser desorption/ionization mass spectrometry	105:149	laser desorption/ionization mass spectrometry	105:149	A flexible and multifunctional metal-organic framework as a matrix for analysis of small molecules using laser desorption/ionization mass spectrometry.
31134988	0	17	theme	metal-organic	31:43	arg1	framework					45:53	A flexible and multifunctional metal-organic framework	0:53	A flexible and multifunctional metal-organic framework as a matrix for analysis of small molecules using laser desorption/ionization mass spectrometry.	0:150	A flexible and multifunctional metal-organic framework as a matrix for analysis of small molecules using laser desorption/ionization mass spectrometry.
31134988	1	18	theme	laser	301:305	arg1	spectrometry					334:345	laser desorption/ionization mass spectrometry	301:345	laser desorption/ionization mass spectrometry	301:345	A variety of functional metal-organic frameworks (MOFs) were prepared via a universal method and served as matrices in small molecule analysis using laser desorption/ionization mass spectrometry.
31134988	2	19	theme	unique	545:550	arg1	effect					567:572	unique size-exclusion effect	545:572	unique size-exclusion effect	545:572	Among them, cysteine-MOF was found to be a multifunctional composite as an excellent matrix and an efficient adsorbent for N-glycans due to its high hydrophilicity, low background interference and unique size-exclusion effect.
31134988	2	19	theme	unique	545:550	arg1	hydrophilicity					497:510	its high hydrophilicity	488:510	its high hydrophilicity	488:510	Among them, cysteine-MOF was found to be a multifunctional composite as an excellent matrix and an efficient adsorbent for N-glycans due to its high hydrophilicity, low background interference and unique size-exclusion effect.
31134988	1	20	theme	desorption/ionization	307:327	arg1	spectrometry					334:345	laser desorption/ionization mass spectrometry	301:345	laser desorption/ionization mass spectrometry	301:345	A variety of functional metal-organic frameworks (MOFs) were prepared via a universal method and served as matrices in small molecule analysis using laser desorption/ionization mass spectrometry.
31134988	1	21	theme	mass	329:332	arg1	spectrometry					334:345	laser desorption/ionization mass spectrometry	301:345	laser desorption/ionization mass spectrometry	301:345	A variety of functional metal-organic frameworks (MOFs) were prepared via a universal method and served as matrices in small molecule analysis using laser desorption/ionization mass spectrometry.
31134988	1	22	theme	universal	228:236	arg1	method					238:243	a universal method	226:243	a universal method	226:243	A variety of functional metal-organic frameworks (MOFs) were prepared via a universal method and served as matrices in small molecule analysis using laser desorption/ionization mass spectrometry.
31134988	1	23	theme	frameworks	190:199	arg1	variety					154:160	A variety	152:160	A variety of functional metal-organic frameworks (MOFs)	152:206	A variety of functional metal-organic frameworks (MOFs) were prepared via a universal method and served as matrices in small molecule analysis using laser desorption/ionization mass spectrometry.
31134988	1	23	theme	frameworks	190:199	arg1	MOFs					202:205	MOFs	202:205	MOFs	202:205	A variety of functional metal-organic frameworks (MOFs) were prepared via a universal method and served as matrices in small molecule analysis using laser desorption/ionization mass spectrometry.
31134988	1	23	theme	frameworks	190:199	arg1	frameworks					190:199	functional metal-organic frameworks	165:199	functional metal-organic frameworks (MOFs)	165:206	A variety of functional metal-organic frameworks (MOFs) were prepared via a universal method and served as matrices in small molecule analysis using laser desorption/ionization mass spectrometry.
31410170	7	0	theme	ratios	999:1004	arg1	balance					984:990	the balance	980:990	the balance of the ratios of IFN-γ/IL-4 and cluster of differentiation (CD)3+/CD19+ cells in Peyer's patches	980:1087	It increased the secretions of secretory immunoglobulin A, interferon (IFN)-γ and interleukin (IL)-4, and maintained the balance of the ratios of IFN-γ/IL-4 and cluster of differentiation (CD)3+/CD19+ cells in Peyer's patches.
31410170	7	1	theme	-γ	938:939	arg1	secretions					880:889	the secretions	876:889	the secretions of secretory immunoglobulin A, interferon (IFN)-γ and interleukin (IL)-4	876:962	It increased the secretions of secretory immunoglobulin A, interferon (IFN)-γ and interleukin (IL)-4, and maintained the balance of the ratios of IFN-γ/IL-4 and cluster of differentiation (CD)3+/CD19+ cells in Peyer's patches.
31410170	3	2	theme	present	366:372	arg1	study					374:378	the present study	362:378	the present study	362:378	(UMHMPS) was investigated in the present study.
31410170	9	3	theme	potential	1277:1285	arg1	use					1287:1289	the potential use	1273:1289	the potential use of UMHMPS as an immune enhancement drug in chemotherapy	1273:1345	Therefore, the present study provides evidence for the potential use of UMHMPS as an immune enhancement drug in chemotherapy.
31410170	5	4	theme	CY-induced	634:643	arg1	model					669:673	a CY-induced immunosuppression mouse model	632:673	a CY-induced immunosuppression mouse model	632:673	The immunomodulatory activity of UMHMPS was evaluated using a CY-induced immunosuppression mouse model.
31410170	7	5	theme	IFN	934:936	arg1	-γ					938:939	interferon (IFN)-γ	922:939	interferon (IFN)-γ	922:939	It increased the secretions of secretory immunoglobulin A, interferon (IFN)-γ and interleukin (IL)-4, and maintained the balance of the ratios of IFN-γ/IL-4 and cluster of differentiation (CD)3+/CD19+ cells in Peyer's patches.
31410170	8	6	theme	TLR	1154:1156	arg1	expression					1120:1129	the expression	1116:1129	the expression of Toll-like receptor (TLR)-4	1116:1159	Furthermore, it increased the expression of Toll-like receptor (TLR)-4, indicating that TLR4 may be one of the receptors of UMHMPS.
31410170	9	7	from	drug	1326:1329	arg1	chemotherapy					1334:1345	chemotherapy	1334:1345	chemotherapy	1334:1345	Therefore, the present study provides evidence for the potential use of UMHMPS as an immune enhancement drug in chemotherapy.
31410170	3	8	dep	investigated	346:357	arg1	UMHMPS					334:339	UMHMPS	334:339	UMHMPS	334:339	(UMHMPS) was investigated in the present study.
31410170	2	9	theme	immunosuppressant	184:200	arg1	effects					202:208	the well-known immunosuppressant effects	169:208	the well-known immunosuppressant effects of cyclophosphamide (CY)	169:233	As a strategy to prevent the well-known immunosuppressant effects of cyclophosphamide (CY), the immunomodulatory activity of the polysaccharide isolated from Urtica macrorrhiza Hand.-Mazz.
31410170	4	10	theme	uronic	447:452	arg1	acid					454:457	uronic acid	447:457	uronic acid	447:457	The chemical properties of UMHMPS, including total carbohydrates, uronic acid, protein contents, monosaccharide compositions, molecular weight and structural confirmation, were investigated.
31410170	7	11	theme	interferon	922:931	arg1	-γ					938:939	interferon (IFN)-γ	922:939	interferon (IFN)-γ	922:939	It increased the secretions of secretory immunoglobulin A, interferon (IFN)-γ and interleukin (IL)-4, and maintained the balance of the ratios of IFN-γ/IL-4 and cluster of differentiation (CD)3+/CD19+ cells in Peyer's patches.
31410170	2	12	theme	well-known	173:182	arg1	effects					202:208	the well-known immunosuppressant effects	169:208	the well-known immunosuppressant effects of cyclophosphamide (CY)	169:233	As a strategy to prevent the well-known immunosuppressant effects of cyclophosphamide (CY), the immunomodulatory activity of the polysaccharide isolated from Urtica macrorrhiza Hand.-Mazz.
31410170	1	13	theme	cyclophosphamide-induced	89:112	arg1	immunosuppression					125:141	cyclophosphamide-induced intestinal immunosuppression	89:141	cyclophosphamide-induced intestinal immunosuppression	89:141	polysaccharides to protect against cyclophosphamide-induced intestinal immunosuppression.
31410170	6	14	theme	galactose	756:764	arg1	UMHMPS					702:707	UMHMPS	702:707	UMHMPS	702:707	The results revealed that UMHMPS, which is composed of rhamnose, gluconic acid, galactose acid, galactose and xylose, exhibited potent immunomodulatory activity and low toxicity in mice.
31410170	6	14	theme	galactose	756:764	arg1	acid					766:769	galactose acid	756:769	galactose acid	756:769	The results revealed that UMHMPS, which is composed of rhamnose, gluconic acid, galactose acid, galactose and xylose, exhibited potent immunomodulatory activity and low toxicity in mice.
31410170	8	15	theme	receptors	1201:1209	arg1	one					1190:1192	one	1190:1192	one	1190:1192	Furthermore, it increased the expression of Toll-like receptor (TLR)-4, indicating that TLR4 may be one of the receptors of UMHMPS.
31410170	8	15	theme	receptors	1201:1209	arg1	receptors					1201:1209	the receptors	1197:1209	the receptors of UMHMPS	1197:1219	Furthermore, it increased the expression of Toll-like receptor (TLR)-4, indicating that TLR4 may be one of the receptors of UMHMPS.
31410170	4	16	theme	structural	528:537	arg1	confirmation					539:550	structural confirmation	528:550	structural confirmation	528:550	The chemical properties of UMHMPS, including total carbohydrates, uronic acid, protein contents, monosaccharide compositions, molecular weight and structural confirmation, were investigated.
31410170	4	17	theme	UMHMPS	408:413	arg1	contents					468:475	protein contents	460:475	protein contents	460:475	The chemical properties of UMHMPS, including total carbohydrates, uronic acid, protein contents, monosaccharide compositions, molecular weight and structural confirmation, were investigated.
31410170	4	17	theme	UMHMPS	408:413	arg1	weight					517:522	molecular weight	507:522	molecular weight	507:522	The chemical properties of UMHMPS, including total carbohydrates, uronic acid, protein contents, monosaccharide compositions, molecular weight and structural confirmation, were investigated.
31410170	4	17	theme	UMHMPS	408:413	arg1	properties					394:403	The chemical properties	381:403	The chemical properties	381:403	The chemical properties of UMHMPS, including total carbohydrates, uronic acid, protein contents, monosaccharide compositions, molecular weight and structural confirmation, were investigated.
31410170	4	17	theme	UMHMPS	408:413	arg1	carbohydrates					432:444	total carbohydrates	426:444	total carbohydrates	426:444	The chemical properties of UMHMPS, including total carbohydrates, uronic acid, protein contents, monosaccharide compositions, molecular weight and structural confirmation, were investigated.
31410170	4	17	theme	UMHMPS	408:413	arg1	confirmation					539:550	structural confirmation	528:550	structural confirmation	528:550	The chemical properties of UMHMPS, including total carbohydrates, uronic acid, protein contents, monosaccharide compositions, molecular weight and structural confirmation, were investigated.
31410170	4	17	theme	UMHMPS	408:413	arg1	acid					454:457	uronic acid	447:457	uronic acid	447:457	The chemical properties of UMHMPS, including total carbohydrates, uronic acid, protein contents, monosaccharide compositions, molecular weight and structural confirmation, were investigated.
31410170	4	17	theme	UMHMPS	408:413	arg1	compositions					493:504	monosaccharide compositions	478:504	monosaccharide compositions	478:504	The chemical properties of UMHMPS, including total carbohydrates, uronic acid, protein contents, monosaccharide compositions, molecular weight and structural confirmation, were investigated.
31410170	1	18	theme	intestinal	114:123	arg1	immunosuppression					125:141	cyclophosphamide-induced intestinal immunosuppression	89:141	cyclophosphamide-induced intestinal immunosuppression	89:141	polysaccharides to protect against cyclophosphamide-induced intestinal immunosuppression.
31410170	7	19	theme	A	919:919	arg1	secretions					880:889	the secretions	876:889	the secretions of secretory immunoglobulin A, interferon (IFN)-γ and interleukin (IL)-4	876:962	It increased the secretions of secretory immunoglobulin A, interferon (IFN)-γ and interleukin (IL)-4, and maintained the balance of the ratios of IFN-γ/IL-4 and cluster of differentiation (CD)3+/CD19+ cells in Peyer's patches.
31410170	0	20	theme	Oral	0:3	arg1	administration					5:18	Oral administration	0:18	Oral administration of Urtica	0:28	Oral administration of Urtica macrorrhiza Hand.-Mazz.
31410170	9	21	theme	UMHMPS	1294:1299	arg1	use					1287:1289	the potential use	1273:1289	the potential use of UMHMPS as an immune enhancement drug in chemotherapy	1273:1345	Therefore, the present study provides evidence for the potential use of UMHMPS as an immune enhancement drug in chemotherapy.
31410170	4	22	theme	total	426:430	arg1	carbohydrates					432:444	total carbohydrates	426:444	total carbohydrates	426:444	The chemical properties of UMHMPS, including total carbohydrates, uronic acid, protein contents, monosaccharide compositions, molecular weight and structural confirmation, were investigated.
31410170	2	23	theme	immunomodulatory	240:255	arg1	activity					257:264	the immunomodulatory activity	236:264	the immunomodulatory activity of the polysaccharide	236:286	As a strategy to prevent the well-known immunosuppressant effects of cyclophosphamide (CY), the immunomodulatory activity of the polysaccharide isolated from Urtica macrorrhiza Hand.-Mazz.
31410170	8	24	theme	UMHMPS	1214:1219	arg1	receptors					1201:1209	the receptors	1197:1209	the receptors of UMHMPS	1197:1219	Furthermore, it increased the expression of Toll-like receptor (TLR)-4, indicating that TLR4 may be one of the receptors of UMHMPS.
31410170	0	25	theme	Urtica	23:28	arg1	administration					5:18	Oral administration	0:18	Oral administration of Urtica	0:28	Oral administration of Urtica macrorrhiza Hand.-Mazz.
31410170	5	26	theme	immunosuppression	645:661	arg1	model					669:673	a CY-induced immunosuppression mouse model	632:673	a CY-induced immunosuppression mouse model	632:673	The immunomodulatory activity of UMHMPS was evaluated using a CY-induced immunosuppression mouse model.
31410170	5	27	theme	mouse	663:667	arg1	model					669:673	a CY-induced immunosuppression mouse model	632:673	a CY-induced immunosuppression mouse model	632:673	The immunomodulatory activity of UMHMPS was evaluated using a CY-induced immunosuppression mouse model.
31410170	6	28	theme	gluconic	741:748	arg1	UMHMPS					702:707	UMHMPS	702:707	UMHMPS	702:707	The results revealed that UMHMPS, which is composed of rhamnose, gluconic acid, galactose acid, galactose and xylose, exhibited potent immunomodulatory activity and low toxicity in mice.
31410170	6	28	theme	gluconic	741:748	arg1	acid					750:753	gluconic acid	741:753	gluconic acid	741:753	The results revealed that UMHMPS, which is composed of rhamnose, gluconic acid, galactose acid, galactose and xylose, exhibited potent immunomodulatory activity and low toxicity in mice.
31410170	6	29	theme	low	841:843	arg1	toxicity					845:852	low toxicity	841:852	low toxicity	841:852	The results revealed that UMHMPS, which is composed of rhamnose, gluconic acid, galactose acid, galactose and xylose, exhibited potent immunomodulatory activity and low toxicity in mice.
31410170	8	30	theme	receptor	1144:1151	arg1	TLR					1154:1156	Toll-like receptor (TLR)-4	1134:1159	Toll-like receptor (TLR)-4	1134:1159	Furthermore, it increased the expression of Toll-like receptor (TLR)-4, indicating that TLR4 may be one of the receptors of UMHMPS.
31410170	9	31	theme	immune	1307:1312	arg1	drug					1326:1329	an immune enhancement drug	1304:1329	an immune enhancement drug in chemotherapy	1304:1345	Therefore, the present study provides evidence for the potential use of UMHMPS as an immune enhancement drug in chemotherapy.
31410170	7	32	theme	cells	1064:1068	arg1	cluster					1024:1030	cluster	1024:1030	cluster	1024:1030	It increased the secretions of secretory immunoglobulin A, interferon (IFN)-γ and interleukin (IL)-4, and maintained the balance of the ratios of IFN-γ/IL-4 and cluster of differentiation (CD)3+/CD19+ cells in Peyer's patches.
31410170	7	32	theme	cells	1064:1068	arg1	IFN-γ/IL-4					1009:1018	IFN-γ/IL-4	1009:1018	IFN-γ/IL-4	1009:1018	It increased the secretions of secretory immunoglobulin A, interferon (IFN)-γ and interleukin (IL)-4, and maintained the balance of the ratios of IFN-γ/IL-4 and cluster of differentiation (CD)3+/CD19+ cells in Peyer's patches.
31410170	4	33	theme	monosaccharide	478:491	arg1	compositions					493:504	monosaccharide compositions	478:504	monosaccharide compositions	478:504	The chemical properties of UMHMPS, including total carbohydrates, uronic acid, protein contents, monosaccharide compositions, molecular weight and structural confirmation, were investigated.
31410170	2	34	theme	polysaccharide	273:286	arg1	activity					257:264	the immunomodulatory activity	236:264	the immunomodulatory activity of the polysaccharide	236:286	As a strategy to prevent the well-known immunosuppressant effects of cyclophosphamide (CY), the immunomodulatory activity of the polysaccharide isolated from Urtica macrorrhiza Hand.-Mazz.
31410170	9	35	theme	enhancement	1314:1324	arg1	drug					1326:1329	an immune enhancement drug	1304:1329	an immune enhancement drug in chemotherapy	1304:1345	Therefore, the present study provides evidence for the potential use of UMHMPS as an immune enhancement drug in chemotherapy.
31410170	4	36	theme	molecular	507:515	arg1	weight					517:522	molecular weight	507:522	molecular weight	507:522	The chemical properties of UMHMPS, including total carbohydrates, uronic acid, protein contents, monosaccharide compositions, molecular weight and structural confirmation, were investigated.
31410170	5	37	theme	UMHMPS	605:610	arg1	activity					593:600	The immunomodulatory activity	572:600	The immunomodulatory activity of UMHMPS	572:610	The immunomodulatory activity of UMHMPS was evaluated using a CY-induced immunosuppression mouse model.
31410170	6	38	from	toxicity	845:852	arg1	mice					857:860	mice	857:860	mice	857:860	The results revealed that UMHMPS, which is composed of rhamnose, gluconic acid, galactose acid, galactose and xylose, exhibited potent immunomodulatory activity and low toxicity in mice.
31410170	6	39	theme	immunomodulatory	811:826	arg1	activity					828:835	potent immunomodulatory activity	804:835	potent immunomodulatory activity	804:835	The results revealed that UMHMPS, which is composed of rhamnose, gluconic acid, galactose acid, galactose and xylose, exhibited potent immunomodulatory activity and low toxicity in mice.
31410170	7	40	theme	3+/CD19+	1055:1062	arg1	cells					1064:1068	differentiation (CD)3+/CD19+ cells	1035:1068	differentiation (CD)3+/CD19+ cells	1035:1068	It increased the secretions of secretory immunoglobulin A, interferon (IFN)-γ and interleukin (IL)-4, and maintained the balance of the ratios of IFN-γ/IL-4 and cluster of differentiation (CD)3+/CD19+ cells in Peyer's patches.
31410170	4	41	theme	protein	460:466	arg1	contents					468:475	protein contents	460:475	protein contents	460:475	The chemical properties of UMHMPS, including total carbohydrates, uronic acid, protein contents, monosaccharide compositions, molecular weight and structural confirmation, were investigated.
31410170	6	42	theme	potent	804:809	arg1	activity					828:835	potent immunomodulatory activity	804:835	potent immunomodulatory activity	804:835	The results revealed that UMHMPS, which is composed of rhamnose, gluconic acid, galactose acid, galactose and xylose, exhibited potent immunomodulatory activity and low toxicity in mice.
31410170	2	43	theme	cyclophosphamide	213:228	arg1	effects					202:208	the well-known immunosuppressant effects	169:208	the well-known immunosuppressant effects of cyclophosphamide (CY)	169:233	As a strategy to prevent the well-known immunosuppressant effects of cyclophosphamide (CY), the immunomodulatory activity of the polysaccharide isolated from Urtica macrorrhiza Hand.-Mazz.
31410170	7	44	theme	IFN-γ/IL-4	1009:1018	arg1	ratios					999:1004	the ratios	995:1004	the ratios of IFN-γ/IL-4 and cluster of differentiation (CD)3+/CD19+ cells in Peyer's patches	995:1087	It increased the secretions of secretory immunoglobulin A, interferon (IFN)-γ and interleukin (IL)-4, and maintained the balance of the ratios of IFN-γ/IL-4 and cluster of differentiation (CD)3+/CD19+ cells in Peyer's patches.
31410170	7	45	theme	cluster	1024:1030	arg1	ratios					999:1004	the ratios	995:1004	the ratios of IFN-γ/IL-4 and cluster of differentiation (CD)3+/CD19+ cells in Peyer's patches	995:1087	It increased the secretions of secretory immunoglobulin A, interferon (IFN)-γ and interleukin (IL)-4, and maintained the balance of the ratios of IFN-γ/IL-4 and cluster of differentiation (CD)3+/CD19+ cells in Peyer's patches.
31410170	6	46	from	activity	828:835	arg1	mice					857:860	mice	857:860	mice	857:860	The results revealed that UMHMPS, which is composed of rhamnose, gluconic acid, galactose acid, galactose and xylose, exhibited potent immunomodulatory activity and low toxicity in mice.
31410170	7	47	theme	IL	958:959	arg1	secretions					880:889	the secretions	876:889	the secretions of secretory immunoglobulin A, interferon (IFN)-γ and interleukin (IL)-4	876:962	It increased the secretions of secretory immunoglobulin A, interferon (IFN)-γ and interleukin (IL)-4, and maintained the balance of the ratios of IFN-γ/IL-4 and cluster of differentiation (CD)3+/CD19+ cells in Peyer's patches.
31410170	4	48	theme	chemical	385:392	arg1	contents					468:475	protein contents	460:475	protein contents	460:475	The chemical properties of UMHMPS, including total carbohydrates, uronic acid, protein contents, monosaccharide compositions, molecular weight and structural confirmation, were investigated.
31410170	4	48	theme	chemical	385:392	arg1	weight					517:522	molecular weight	507:522	molecular weight	507:522	The chemical properties of UMHMPS, including total carbohydrates, uronic acid, protein contents, monosaccharide compositions, molecular weight and structural confirmation, were investigated.
31410170	4	48	theme	chemical	385:392	arg1	properties					394:403	The chemical properties	381:403	The chemical properties	381:403	The chemical properties of UMHMPS, including total carbohydrates, uronic acid, protein contents, monosaccharide compositions, molecular weight and structural confirmation, were investigated.
31410170	4	48	theme	chemical	385:392	arg1	carbohydrates					432:444	total carbohydrates	426:444	total carbohydrates	426:444	The chemical properties of UMHMPS, including total carbohydrates, uronic acid, protein contents, monosaccharide compositions, molecular weight and structural confirmation, were investigated.
31410170	4	48	theme	chemical	385:392	arg1	confirmation					539:550	structural confirmation	528:550	structural confirmation	528:550	The chemical properties of UMHMPS, including total carbohydrates, uronic acid, protein contents, monosaccharide compositions, molecular weight and structural confirmation, were investigated.
31410170	4	48	theme	chemical	385:392	arg1	acid					454:457	uronic acid	447:457	uronic acid	447:457	The chemical properties of UMHMPS, including total carbohydrates, uronic acid, protein contents, monosaccharide compositions, molecular weight and structural confirmation, were investigated.
31410170	4	48	theme	chemical	385:392	arg1	compositions					493:504	monosaccharide compositions	478:504	monosaccharide compositions	478:504	The chemical properties of UMHMPS, including total carbohydrates, uronic acid, protein contents, monosaccharide compositions, molecular weight and structural confirmation, were investigated.
31410170	7	49	theme	secretory	894:902	arg1	A					919:919	secretory immunoglobulin A	894:919	secretory immunoglobulin A	894:919	It increased the secretions of secretory immunoglobulin A, interferon (IFN)-γ and interleukin (IL)-4, and maintained the balance of the ratios of IFN-γ/IL-4 and cluster of differentiation (CD)3+/CD19+ cells in Peyer's patches.
31410170	7	50	theme	immunoglobulin	904:917	arg1	A					919:919	secretory immunoglobulin A	894:919	secretory immunoglobulin A	894:919	It increased the secretions of secretory immunoglobulin A, interferon (IFN)-γ and interleukin (IL)-4, and maintained the balance of the ratios of IFN-γ/IL-4 and cluster of differentiation (CD)3+/CD19+ cells in Peyer's patches.
31410170	8	51	theme	Toll-like	1134:1142	arg1	TLR					1154:1156	Toll-like receptor (TLR)-4	1134:1159	Toll-like receptor (TLR)-4	1134:1159	Furthermore, it increased the expression of Toll-like receptor (TLR)-4, indicating that TLR4 may be one of the receptors of UMHMPS.
31410170	9	52	theme	present	1237:1243	arg1	study					1245:1249	the present study	1233:1249	the present study	1233:1249	Therefore, the present study provides evidence for the potential use of UMHMPS as an immune enhancement drug in chemotherapy.
31410170	5	53	theme	immunomodulatory	576:591	arg1	activity					593:600	The immunomodulatory activity	572:600	The immunomodulatory activity of UMHMPS	572:610	The immunomodulatory activity of UMHMPS was evaluated using a CY-induced immunosuppression mouse model.
31410170	7	54	theme	interleukin	945:955	arg1	IL					958:959	interleukin (IL)-4	945:962	interleukin (IL)-4	945:962	It increased the secretions of secretory immunoglobulin A, interferon (IFN)-γ and interleukin (IL)-4, and maintained the balance of the ratios of IFN-γ/IL-4 and cluster of differentiation (CD)3+/CD19+ cells in Peyer's patches.
31329533	11	0	theme	hybridisation	1284:1296	arg1	values					1298:1303	The digital DNA-DNA hybridisation values	1264:1303	The digital DNA-DNA hybridisation values between strain NEAU-SA2T and its most closely related species	1264:1365	The digital DNA-DNA hybridisation values between strain NEAU-SA2T and its most closely related species were 37.00 and 41.10 %, respectively, again indicating that they belong to different taxa.
31329533	11	0	theme	hybridisation	1284:1296	arg1	%					1388:1388	37.00 and 41.10 %	1372:1388	37.00 and 41.10 %	1372:1388	The digital DNA-DNA hybridisation values between strain NEAU-SA2T and its most closely related species were 37.00 and 41.10 %, respectively, again indicating that they belong to different taxa.
31329533	12	1	theme	Arthrobacter	1526:1537	arg1	species					1505:1511	a novel species	1497:1511	a novel species	1497:1511	Therefore, strain NEAU-SA2T represents a novel species of the genus Arthrobacter, for which the name Arthrobacter celericrescens sp.
31329533	7	2	theme	polar	858:862	arg1	lipids					864:869	The polar lipids	854:869	The polar lipids	854:869	The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unidentified glycolipid.
31329533	12	3	theme	celericrescens	1572:1585	arg1	sp					1587:1588	the name Arthrobacter celericrescens sp	1550:1588	the name Arthrobacter celericrescens sp	1550:1588	Therefore, strain NEAU-SA2T represents a novel species of the genus Arthrobacter, for which the name Arthrobacter celericrescens sp.
31329533	14	4	theme	=DSM	1639:1642	arg1	2017272T					1661:1668	=DSM 106718T=CCTCC AB 2017272T	1639:1668	=DSM 106718T=CCTCC AB 2017272T	1639:1668	The type strain is NEAU-SA2T (=DSM 106718T=CCTCC AB 2017272T).
31329533	14	4	theme	=DSM	1639:1642	arg1	NEAU-SA2T					1628:1636	NEAU-SA2T	1628:1636	NEAU-SA2T (=DSM 106718T=CCTCC AB 2017272T)	1628:1669	The type strain is NEAU-SA2T (=DSM 106718T=CCTCC AB 2017272T).
31329533	6	5	theme	fatty	741:745	arg1	anteiso-C15 					758:769	anteiso-C15 	758:769	anteiso-C15 	758:769	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and C16 : 0; MK-9(H2) was the predominant respiratory quinone.
31329533	6	5	theme	fatty	741:745	arg1	acids					747:751	The major cellular fatty acids	722:751	The major cellular fatty acids	722:751	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and C16 : 0; MK-9(H2) was the predominant respiratory quinone.
31329533	12	6	theme	Arthrobacter	1559:1570	arg1	sp					1587:1588	the name Arthrobacter celericrescens sp	1550:1588	the name Arthrobacter celericrescens sp	1550:1588	Therefore, strain NEAU-SA2T represents a novel species of the genus Arthrobacter, for which the name Arthrobacter celericrescens sp.
31329533	6	7	theme	cellular	732:739	arg1	anteiso-C15 					758:769	anteiso-C15 	758:769	anteiso-C15 	758:769	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and C16 : 0; MK-9(H2) was the predominant respiratory quinone.
31329533	6	7	theme	cellular	732:739	arg1	acids					747:751	The major cellular fatty acids	722:751	The major cellular fatty acids	722:751	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and C16 : 0; MK-9(H2) was the predominant respiratory quinone.
31329533	1	8	theme	forest	52:57	arg1	soil					59:62	forest soil	52:62	forest soil	52:62	nov., isolated from forest soil.
31329533	6	9	theme	major	726:730	arg1	anteiso-C15 					758:769	anteiso-C15 	758:769	anteiso-C15 	758:769	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and C16 : 0; MK-9(H2) was the predominant respiratory quinone.
31329533	6	9	theme	major	726:730	arg1	acids					747:751	The major cellular fatty acids	722:751	The major cellular fatty acids	722:751	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and C16 : 0; MK-9(H2) was the predominant respiratory quinone.
31329533	14	10	theme	AB	1658:1659	arg1	2017272T					1661:1668	=DSM 106718T=CCTCC AB 2017272T	1639:1668	=DSM 106718T=CCTCC AB 2017272T	1639:1668	The type strain is NEAU-SA2T (=DSM 106718T=CCTCC AB 2017272T).
31329533	14	10	theme	AB	1658:1659	arg1	NEAU-SA2T					1628:1636	NEAU-SA2T	1628:1636	NEAU-SA2T (=DSM 106718T=CCTCC AB 2017272T)	1628:1669	The type strain is NEAU-SA2T (=DSM 106718T=CCTCC AB 2017272T).
31329533	5	11	theme	16S	439:441	arg1	rRNA					443:446	the 16S rRNA	435:446	the 16S rRNA gene sequence	435:460	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Arthrobacter and was closely related to Arthrobacter cupressi DSM 24664T (98.89 %) and Arthrobacter silvisoli CCTCC AB 2017271T (98.41 %), which was further confirmed by multilocus sequence analysis.
31329533	11	12	theme	related	1351:1357	arg1	species					1359:1365	its most closely related species	1334:1365	its most closely related species	1334:1365	The digital DNA-DNA hybridisation values between strain NEAU-SA2T and its most closely related species were 37.00 and 41.10 %, respectively, again indicating that they belong to different taxa.
31329533	2	13	theme	forest	131:136	arg1	soil					138:141	forest soil	131:141	forest soil collected from the Zhangjiajie city, Hunan Province, PR China	131:203	A novel bacterial strain, designated NEAU-SA2T, was isolated from forest soil collected from the Zhangjiajie city, Hunan Province, PR China and characterised using a polyphasic approach.
31329533	5	14	theme	Arthrobacter	562:573	arg1	DSM					584:586	Arthrobacter cupressi DSM 24664T	562:593	Arthrobacter cupressi DSM 24664T (98.89 %)	562:603	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Arthrobacter and was closely related to Arthrobacter cupressi DSM 24664T (98.89 %) and Arthrobacter silvisoli CCTCC AB 2017271T (98.41 %), which was further confirmed by multilocus sequence analysis.
31329533	5	14	theme	Arthrobacter	562:573	arg1	%					602:602	98.89 %	596:602	98.89 %	596:602	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Arthrobacter and was closely related to Arthrobacter cupressi DSM 24664T (98.89 %) and Arthrobacter silvisoli CCTCC AB 2017271T (98.41 %), which was further confirmed by multilocus sequence analysis.
31329533	11	15	theme	different	1442:1450	arg1	taxa					1452:1455	different taxa	1442:1455	different taxa	1442:1455	The digital DNA-DNA hybridisation values between strain NEAU-SA2T and its most closely related species were 37.00 and 41.10 %, respectively, again indicating that they belong to different taxa.
31329533	5	16	theme	rRNA	443:446	arg1	sequence					453:460	the 16S rRNA gene sequence	435:460	the 16S rRNA gene sequence	435:460	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Arthrobacter and was closely related to Arthrobacter cupressi DSM 24664T (98.89 %) and Arthrobacter silvisoli CCTCC AB 2017271T (98.41 %), which was further confirmed by multilocus sequence analysis.
31329533	12	17	theme	novel	1499:1503	arg1	species					1505:1511	a novel species	1497:1511	a novel species	1497:1511	Therefore, strain NEAU-SA2T represents a novel species of the genus Arthrobacter, for which the name Arthrobacter celericrescens sp.
31329533	5	18	theme	cupressi	575:582	arg1	DSM					584:586	Arthrobacter cupressi DSM 24664T	562:593	Arthrobacter cupressi DSM 24664T (98.89 %)	562:603	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Arthrobacter and was closely related to Arthrobacter cupressi DSM 24664T (98.89 %) and Arthrobacter silvisoli CCTCC AB 2017271T (98.41 %), which was further confirmed by multilocus sequence analysis.
31329533	5	18	theme	cupressi	575:582	arg1	%					602:602	98.89 %	596:602	98.89 %	596:602	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Arthrobacter and was closely related to Arthrobacter cupressi DSM 24664T (98.89 %) and Arthrobacter silvisoli CCTCC AB 2017271T (98.41 %), which was further confirmed by multilocus sequence analysis.
31329533	9	19	theme	67.04 mol	1113:1121	arg1	%					1122:1122	67.04 mol%	1113:1122	67.04 mol%	1113:1122	The genomic G+C content of strain NEAU-SA2T was 67.04 mol%.
31329533	9	19	theme	67.04 mol	1113:1121	arg1	G+C content					1077:1087	The genomic G+C content	1065:1087	The genomic G+C content of strain NEAU-SA2T	1065:1107	The genomic G+C content of strain NEAU-SA2T was 67.04 mol%.
31329533	5	20	theme	gene	448:451	arg1	sequence					453:460	the 16S rRNA gene sequence	435:460	the 16S rRNA gene sequence	435:460	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Arthrobacter and was closely related to Arthrobacter cupressi DSM 24664T (98.89 %) and Arthrobacter silvisoli CCTCC AB 2017271T (98.41 %), which was further confirmed by multilocus sequence analysis.
31329533	12	21	theme	genus	1520:1524	arg1	Arthrobacter					1526:1537	the genus Arthrobacter	1516:1537	the genus Arthrobacter	1516:1537	Therefore, strain NEAU-SA2T represents a novel species of the genus Arthrobacter, for which the name Arthrobacter celericrescens sp.
31329533	8	22	theme	peptidoglycan	984:996	arg1	A3α					1007:1009	A3α	1007:1009	A3α	1007:1009	The peptidoglycan type was A3α, and the cell-wall sugars were glucose and galactose.
31329533	8	22	theme	peptidoglycan	984:996	arg1	type					998:1001	The peptidoglycan type	980:1001	The peptidoglycan type	980:1001	The peptidoglycan type was A3α, and the cell-wall sugars were glucose and galactose.
31329533	5	23	theme	genus	516:520	arg1	Arthrobacter					522:533	the genus Arthrobacter	512:533	the genus Arthrobacter	512:533	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Arthrobacter and was closely related to Arthrobacter cupressi DSM 24664T (98.89 %) and Arthrobacter silvisoli CCTCC AB 2017271T (98.41 %), which was further confirmed by multilocus sequence analysis.
31329533	7	24	theme	unidentified	955:966	arg1	glycolipid					968:977	an unidentified glycolipid	952:977	an unidentified glycolipid	952:977	The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unidentified glycolipid.
31329533	0	25	theme	Arthrobacter	0:11	arg1	celericrescens					13:26	Arthrobacter celericrescens	0:26	Arthrobacter celericrescens	0:26	Arthrobacter celericrescens sp.
31329533	2	26	theme	Province	186:193	arg1	China					199:203	the Zhangjiajie city, Hunan Province, PR China	158:203	China	199:203	A novel bacterial strain, designated NEAU-SA2T, was isolated from forest soil collected from the Zhangjiajie city, Hunan Province, PR China and characterised using a polyphasic approach.
31329533	2	26	theme	Province	186:193	arg1	Hunan					180:184	Hunan	180:184	Hunan	180:184	A novel bacterial strain, designated NEAU-SA2T, was isolated from forest soil collected from the Zhangjiajie city, Hunan Province, PR China and characterised using a polyphasic approach.
31329533	10	27	theme	AB	1232:1233	arg1	2017271T					1235:1242	CCTCC AB 2017271T	1226:1242	CCTCC AB 2017271T	1226:1242	The average nucleotide identity values between NEAU-SA2T and A. cupressi DSM 24664T and A. silvisoli CCTCC AB 2017271T were 88.57-90.94 %.
31329533	5	28	theme	AB	638:639	arg1	2017271T					641:648	Arthrobacter silvisoli CCTCC AB 2017271T	609:648	Arthrobacter silvisoli CCTCC AB 2017271T (98.41 %)	609:658	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Arthrobacter and was closely related to Arthrobacter cupressi DSM 24664T (98.89 %) and Arthrobacter silvisoli CCTCC AB 2017271T (98.41 %), which was further confirmed by multilocus sequence analysis.
31329533	5	28	theme	AB	638:639	arg1	%					657:657	98.41 %	651:657	98.41 %	651:657	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Arthrobacter and was closely related to Arthrobacter cupressi DSM 24664T (98.89 %) and Arthrobacter silvisoli CCTCC AB 2017271T (98.41 %), which was further confirmed by multilocus sequence analysis.
31329533	9	29	theme	strain	1092:1097	arg1	NEAU-SA2T					1099:1107	strain NEAU-SA2T	1092:1107	strain NEAU-SA2T	1092:1107	The genomic G+C content of strain NEAU-SA2T was 67.04 mol%.
31329533	10	30	theme	CCTCC	1226:1230	arg1	2017271T					1235:1242	CCTCC AB 2017271T	1226:1242	CCTCC AB 2017271T	1226:1242	The average nucleotide identity values between NEAU-SA2T and A. cupressi DSM 24664T and A. silvisoli CCTCC AB 2017271T were 88.57-90.94 %.
31329533	6	31	theme	respiratory	833:843	arg1	quinone					845:851	the predominant respiratory quinone	817:851	the predominant respiratory quinone	817:851	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and C16 : 0; MK-9(H2) was the predominant respiratory quinone.
31329533	6	31	theme	respiratory	833:843	arg1	 0					771:772	 0	771:772	 0	771:772	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and C16 : 0; MK-9(H2) was the predominant respiratory quinone.
31329533	2	32	theme	Zhangjiajie	162:172	arg1	China					199:203	the Zhangjiajie city, Hunan Province, PR China	158:203	China	199:203	A novel bacterial strain, designated NEAU-SA2T, was isolated from forest soil collected from the Zhangjiajie city, Hunan Province, PR China and characterised using a polyphasic approach.
31329533	2	32	theme	Zhangjiajie	162:172	arg1	Hunan					180:184	Hunan	180:184	Hunan	180:184	A novel bacterial strain, designated NEAU-SA2T, was isolated from forest soil collected from the Zhangjiajie city, Hunan Province, PR China and characterised using a polyphasic approach.
31329533	5	33	theme	multilocus	692:701	arg1	analysis					712:719	multilocus sequence analysis	692:719	multilocus sequence analysis	692:719	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Arthrobacter and was closely related to Arthrobacter cupressi DSM 24664T (98.89 %) and Arthrobacter silvisoli CCTCC AB 2017271T (98.41 %), which was further confirmed by multilocus sequence analysis.
31329533	6	34	theme	predominant	821:831	arg1	quinone					845:851	the predominant respiratory quinone	817:851	the predominant respiratory quinone	817:851	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and C16 : 0; MK-9(H2) was the predominant respiratory quinone.
31329533	6	34	theme	predominant	821:831	arg1	 0					771:772	 0	771:772	 0	771:772	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and C16 : 0; MK-9(H2) was the predominant respiratory quinone.
31329533	14	35	theme	type	1613:1616	arg1	NEAU-SA2T					1628:1636	NEAU-SA2T	1628:1636	NEAU-SA2T (=DSM 106718T=CCTCC AB 2017272T)	1628:1669	The type strain is NEAU-SA2T (=DSM 106718T=CCTCC AB 2017272T).
31329533	14	35	theme	type	1613:1616	arg1	strain					1618:1623	The type strain	1609:1623	The type strain	1609:1623	The type strain is NEAU-SA2T (=DSM 106718T=CCTCC AB 2017272T).
31329533	10	36	theme	average	1129:1135	arg1	identity					1148:1155	average nucleotide identity	1129:1155	The average nucleotide identity values between NEAU-SA2T and A. cupressi DSM 24664T and A. silvisoli CCTCC AB 2017271T	1125:1242	The average nucleotide identity values between NEAU-SA2T and A. cupressi DSM 24664T and A. silvisoli CCTCC AB 2017271T were 88.57-90.94 %.
31329533	5	37	theme	sequence	703:710	arg1	analysis					712:719	multilocus sequence analysis	692:719	multilocus sequence analysis	692:719	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Arthrobacter and was closely related to Arthrobacter cupressi DSM 24664T (98.89 %) and Arthrobacter silvisoli CCTCC AB 2017271T (98.41 %), which was further confirmed by multilocus sequence analysis.
31329533	11	38	theme	DNA-DNA	1276:1282	arg1	values					1298:1303	The digital DNA-DNA hybridisation values	1264:1303	The digital DNA-DNA hybridisation values between strain NEAU-SA2T and its most closely related species	1264:1365	The digital DNA-DNA hybridisation values between strain NEAU-SA2T and its most closely related species were 37.00 and 41.10 %, respectively, again indicating that they belong to different taxa.
31329533	11	38	theme	DNA-DNA	1276:1282	arg1	%					1388:1388	37.00 and 41.10 %	1372:1388	37.00 and 41.10 %	1372:1388	The digital DNA-DNA hybridisation values between strain NEAU-SA2T and its most closely related species were 37.00 and 41.10 %, respectively, again indicating that they belong to different taxa.
31329533	12	39	theme	name	1554:1557	arg1	sp					1587:1588	the name Arthrobacter celericrescens sp	1550:1588	the name Arthrobacter celericrescens sp	1550:1588	Therefore, strain NEAU-SA2T represents a novel species of the genus Arthrobacter, for which the name Arthrobacter celericrescens sp.
31329533	2	40	dep	China	199:203	arg1	PR					196:197	the Zhangjiajie city, Hunan Province, PR China	158:203	PR	196:197	A novel bacterial strain, designated NEAU-SA2T, was isolated from forest soil collected from the Zhangjiajie city, Hunan Province, PR China and characterised using a polyphasic approach.
31329533	11	41	theme	digital	1268:1274	arg1	values					1298:1303	The digital DNA-DNA hybridisation values	1264:1303	The digital DNA-DNA hybridisation values between strain NEAU-SA2T and its most closely related species	1264:1365	The digital DNA-DNA hybridisation values between strain NEAU-SA2T and its most closely related species were 37.00 and 41.10 %, respectively, again indicating that they belong to different taxa.
31329533	11	41	theme	digital	1268:1274	arg1	%					1388:1388	37.00 and 41.10 %	1372:1388	37.00 and 41.10 %	1372:1388	The digital DNA-DNA hybridisation values between strain NEAU-SA2T and its most closely related species were 37.00 and 41.10 %, respectively, again indicating that they belong to different taxa.
31329533	6	42	dep	 0	771:772	arg1	C16 					795:798	C16 	795:798	C16 	795:798	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and C16 : 0; MK-9(H2) was the predominant respiratory quinone.
31329533	6	42	dep	 0	771:772	arg1	 0					788:789	 0	788:789	 0	788:789	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and C16 : 0; MK-9(H2) was the predominant respiratory quinone.
31329533	6	42	dep	 0	771:772	arg1	MK-9					804:807	MK-9	804:807	MK-9(H2)	804:811	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and C16 : 0; MK-9(H2) was the predominant respiratory quinone.
31329533	6	42	dep	 0	771:772	arg1	H2					809:810	H2	809:810	H2	809:810	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and C16 : 0; MK-9(H2) was the predominant respiratory quinone.
31329533	6	42	dep	 0	771:772	arg1	 0					800:801	 0	800:801	 0	800:801	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and C16 : 0; MK-9(H2) was the predominant respiratory quinone.
31329533	9	43	theme	NEAU-SA2T	1099:1107	arg1	%					1122:1122	67.04 mol%	1113:1122	67.04 mol%	1113:1122	The genomic G+C content of strain NEAU-SA2T was 67.04 mol%.
31329533	9	43	theme	NEAU-SA2T	1099:1107	arg1	G+C content					1077:1087	The genomic G+C content	1065:1087	The genomic G+C content of strain NEAU-SA2T	1065:1107	The genomic G+C content of strain NEAU-SA2T was 67.04 mol%.
31329533	2	44	theme	bacterial	73:81	arg1	strain					83:88	A novel bacterial strain	65:88	A novel bacterial strain	65:88	A novel bacterial strain, designated NEAU-SA2T, was isolated from forest soil collected from the Zhangjiajie city, Hunan Province, PR China and characterised using a polyphasic approach.
31329533	5	45	theme	Arthrobacter	609:620	arg1	2017271T					641:648	Arthrobacter silvisoli CCTCC AB 2017271T	609:648	Arthrobacter silvisoli CCTCC AB 2017271T (98.41 %)	609:658	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Arthrobacter and was closely related to Arthrobacter cupressi DSM 24664T (98.89 %) and Arthrobacter silvisoli CCTCC AB 2017271T (98.41 %), which was further confirmed by multilocus sequence analysis.
31329533	5	45	theme	Arthrobacter	609:620	arg1	%					657:657	98.41 %	651:657	98.41 %	651:657	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Arthrobacter and was closely related to Arthrobacter cupressi DSM 24664T (98.89 %) and Arthrobacter silvisoli CCTCC AB 2017271T (98.41 %), which was further confirmed by multilocus sequence analysis.
31329533	4	46	theme	%	390:390	arg1	NaCl					392:395	0 % NaCl	388:395	0 % NaCl (w/v)	388:401	The strain grew optimally at 28 °C, pH 7.0 and with 0 % NaCl (w/v).
31329533	4	46	theme	%	390:390	arg1	w/v					398:400	w/v	398:400	w/v	398:400	The strain grew optimally at 28 °C, pH 7.0 and with 0 % NaCl (w/v).
31329533	2	47	theme	novel	67:71	arg1	strain					83:88	A novel bacterial strain	65:88	A novel bacterial strain	65:88	A novel bacterial strain, designated NEAU-SA2T, was isolated from forest soil collected from the Zhangjiajie city, Hunan Province, PR China and characterised using a polyphasic approach.
31329533	5	48	theme	silvisoli	622:630	arg1	2017271T					641:648	Arthrobacter silvisoli CCTCC AB 2017271T	609:648	Arthrobacter silvisoli CCTCC AB 2017271T (98.41 %)	609:658	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Arthrobacter and was closely related to Arthrobacter cupressi DSM 24664T (98.89 %) and Arthrobacter silvisoli CCTCC AB 2017271T (98.41 %), which was further confirmed by multilocus sequence analysis.
31329533	5	48	theme	silvisoli	622:630	arg1	%					657:657	98.41 %	651:657	98.41 %	651:657	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Arthrobacter and was closely related to Arthrobacter cupressi DSM 24664T (98.89 %) and Arthrobacter silvisoli CCTCC AB 2017271T (98.41 %), which was further confirmed by multilocus sequence analysis.
31329533	4	49	theme	0 	388:389	arg1	NaCl					392:395	0 % NaCl	388:395	0 % NaCl (w/v)	388:401	The strain grew optimally at 28 °C, pH 7.0 and with 0 % NaCl (w/v).
31329533	4	49	theme	0 	388:389	arg1	w/v					398:400	w/v	398:400	w/v	398:400	The strain grew optimally at 28 °C, pH 7.0 and with 0 % NaCl (w/v).
31329533	2	50	theme	polyphasic	231:240	arg1	approach					242:249	a polyphasic approach	229:249	a polyphasic approach	229:249	A novel bacterial strain, designated NEAU-SA2T, was isolated from forest soil collected from the Zhangjiajie city, Hunan Province, PR China and characterised using a polyphasic approach.
31329533	8	51	theme	cell-wall	1020:1028	arg1	glucose					1042:1048	glucose	1042:1048	glucose	1042:1048	The peptidoglycan type was A3α, and the cell-wall sugars were glucose and galactose.
31329533	8	51	theme	cell-wall	1020:1028	arg1	sugars					1030:1035	the cell-wall sugars	1016:1035	the cell-wall sugars	1016:1035	The peptidoglycan type was A3α, and the cell-wall sugars were glucose and galactose.
31329533	2	52	attach	isolated	117:124	arg2	strain					83:88	A novel bacterial strain	65:88	A novel bacterial strain	65:88	A novel bacterial strain, designated NEAU-SA2T, was isolated from forest soil collected from the Zhangjiajie city, Hunan Province, PR China and characterised using a polyphasic approach.
31329533	2	52	attach	isolated	117:124	arg1	soil					138:141	forest soil	131:141	forest soil collected from the Zhangjiajie city, Hunan Province, PR China	131:203	A novel bacterial strain, designated NEAU-SA2T, was isolated from forest soil collected from the Zhangjiajie city, Hunan Province, PR China and characterised using a polyphasic approach.
31329533	10	53	theme	identity	1148:1155	arg1	values					1157:1162	The average nucleotide identity values	1125:1162	The average nucleotide identity values between NEAU-SA2T and A. cupressi DSM 24664T and A. silvisoli CCTCC AB 2017271T	1125:1242	The average nucleotide identity values between NEAU-SA2T and A. cupressi DSM 24664T and A. silvisoli CCTCC AB 2017271T were 88.57-90.94 %.
31329533	10	53	theme	identity	1148:1155	arg1	%					1261:1261	88.57-90.94 %	1249:1261	88.57-90.94 %	1249:1261	The average nucleotide identity values between NEAU-SA2T and A. cupressi DSM 24664T and A. silvisoli CCTCC AB 2017271T were 88.57-90.94 %.
31329533	5	54	theme	CCTCC	632:636	arg1	2017271T					641:648	Arthrobacter silvisoli CCTCC AB 2017271T	609:648	Arthrobacter silvisoli CCTCC AB 2017271T (98.41 %)	609:658	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Arthrobacter and was closely related to Arthrobacter cupressi DSM 24664T (98.89 %) and Arthrobacter silvisoli CCTCC AB 2017271T (98.41 %), which was further confirmed by multilocus sequence analysis.
31329533	5	54	theme	CCTCC	632:636	arg1	%					657:657	98.41 %	651:657	98.41 %	651:657	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Arthrobacter and was closely related to Arthrobacter cupressi DSM 24664T (98.89 %) and Arthrobacter silvisoli CCTCC AB 2017271T (98.41 %), which was further confirmed by multilocus sequence analysis.
31329533	0	55	dep	sp	28:29	arg1	celericrescens					13:26	Arthrobacter celericrescens	0:26	Arthrobacter celericrescens	0:26	Arthrobacter celericrescens sp.
31329533	10	56	theme	nucleotide	1137:1146	arg1	identity					1148:1155	average nucleotide identity	1129:1155	The average nucleotide identity values between NEAU-SA2T and A. cupressi DSM 24664T and A. silvisoli CCTCC AB 2017271T	1125:1242	The average nucleotide identity values between NEAU-SA2T and A. cupressi DSM 24664T and A. silvisoli CCTCC AB 2017271T were 88.57-90.94 %.
31329533	10	57	theme	DSM	1198:1200	arg1	24664T					1202:1207	DSM 24664T	1198:1207	DSM 24664T	1198:1207	The average nucleotide identity values between NEAU-SA2T and A. cupressi DSM 24664T and A. silvisoli CCTCC AB 2017271T were 88.57-90.94 %.
31329533	2	58	theme	city	174:177	arg1	China					199:203	the Zhangjiajie city, Hunan Province, PR China	158:203	China	199:203	A novel bacterial strain, designated NEAU-SA2T, was isolated from forest soil collected from the Zhangjiajie city, Hunan Province, PR China and characterised using a polyphasic approach.
31329533	2	58	theme	city	174:177	arg1	Hunan					180:184	Hunan	180:184	Hunan	180:184	A novel bacterial strain, designated NEAU-SA2T, was isolated from forest soil collected from the Zhangjiajie city, Hunan Province, PR China and characterised using a polyphasic approach.
31329533	5	59	theme	Phylogenetic	404:415	arg1	analysis					417:424	Phylogenetic analysis	404:424	Phylogenetic analysis based on the 16S rRNA gene sequence	404:460	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Arthrobacter and was closely related to Arthrobacter cupressi DSM 24664T (98.89 %) and Arthrobacter silvisoli CCTCC AB 2017271T (98.41 %), which was further confirmed by multilocus sequence analysis.
31329533	10	60	dep	NEAU-SA2T	1172:1180	arg1	24664T					1202:1207	DSM 24664T	1198:1207	DSM 24664T	1198:1207	The average nucleotide identity values between NEAU-SA2T and A. cupressi DSM 24664T and A. silvisoli CCTCC AB 2017271T were 88.57-90.94 %.
31329533	10	60	dep	NEAU-SA2T	1172:1180	arg1	2017271T					1235:1242	CCTCC AB 2017271T	1226:1242	CCTCC AB 2017271T	1226:1242	The average nucleotide identity values between NEAU-SA2T and A. cupressi DSM 24664T and A. silvisoli CCTCC AB 2017271T were 88.57-90.94 %.
31329533	11	61	theme	strain	1313:1318	arg1	NEAU-SA2T					1320:1328	strain NEAU-SA2T	1313:1328	strain NEAU-SA2T	1313:1328	The digital DNA-DNA hybridisation values between strain NEAU-SA2T and its most closely related species were 37.00 and 41.10 %, respectively, again indicating that they belong to different taxa.
31329533	14	62	theme	106718T=CCTCC	1644:1656	arg1	2017272T					1661:1668	=DSM 106718T=CCTCC AB 2017272T	1639:1668	=DSM 106718T=CCTCC AB 2017272T	1639:1668	The type strain is NEAU-SA2T (=DSM 106718T=CCTCC AB 2017272T).
31329533	14	62	theme	106718T=CCTCC	1644:1656	arg1	NEAU-SA2T					1628:1636	NEAU-SA2T	1628:1636	NEAU-SA2T (=DSM 106718T=CCTCC AB 2017272T)	1628:1669	The type strain is NEAU-SA2T (=DSM 106718T=CCTCC AB 2017272T).
31329533	9	63	theme	genomic	1069:1075	arg1	%					1122:1122	67.04 mol%	1113:1122	67.04 mol%	1113:1122	The genomic G+C content of strain NEAU-SA2T was 67.04 mol%.
31329533	9	63	theme	genomic	1069:1075	arg1	G+C content					1077:1087	The genomic G+C content	1065:1087	The genomic G+C content of strain NEAU-SA2T	1065:1107	The genomic G+C content of strain NEAU-SA2T was 67.04 mol%.
31329533	12	64	theme	strain	1469:1474	arg1	NEAU-SA2T					1476:1484	strain NEAU-SA2T	1469:1484	strain NEAU-SA2T	1469:1484	Therefore, strain NEAU-SA2T represents a novel species of the genus Arthrobacter, for which the name Arthrobacter celericrescens sp.
30287380	8	0	theme	Porphyromonas	1379:1391	arg1	gingivalis					1393:1402	Porphyromonas gingivalis	1379:1402	Porphyromonas gingivalis under high glucose concentration	1379:1435	What's more, it can sense the ambient glucose stimulus, rapidly and correspondingly adjust its inner pore structure to control the loaded metronidazole release, lead to an improved antimicrobial activity against Porphyromonas gingivalis under high glucose concentration.
30287380	1	1	with	system	187:192	arg1	release					210:216	controlled release	199:216	controlled release of metronidazole	199:233	A novel glucose-sensitive drug delivery system with controlled release of metronidazole was synthesized by using biocompatible photo-crosslinked chitosan hydrogel film.
30287380	7	2	theme	better	1108:1113	arg1	biocompatibility					1115:1130	better biocompatibility	1108:1130	better biocompatibility than chemical crosslinked samples	1108:1164	In addition, this material possesses better biocompatibility than chemical crosslinked samples.
30287380	8	3	theme	pore	1268:1271	arg1	structure					1273:1281	its inner pore structure	1258:1281	its inner pore structure	1258:1281	What's more, it can sense the ambient glucose stimulus, rapidly and correspondingly adjust its inner pore structure to control the loaded metronidazole release, lead to an improved antimicrobial activity against Porphyromonas gingivalis under high glucose concentration.
30287380	6	4	theme	high	1034:1037	arg1	level					1039:1043	a relatively high level	1021:1043	a relatively high level	1021:1043	The results show that this photo-crosslinked hydrogel film has good mechanical properties; meanwhile, the immobilized enzyme's bonding capacity and activity can maintain a relatively high level after surface activation.
30287380	9	5	from	therapy	1535:1541	arg1	clinic					1546:1551	clinic	1546:1551	clinic	1546:1551	This glucose sensitive hydrogel film may provide a promising method for diabetic's periodontitis therapy in clinic.
30287380	2	6	theme	molecular	414:422	arg1	chains					424:429	chitosan molecular chains	405:429	chitosan molecular chains	405:429	Specifically, methacrylic anhydride, as photosensitive substance, was firstly grafted on chitosan molecular chains and then UV irradiation was applied as the crosslinking method.
30287380	8	7	theme	antimicrobial	1348:1360	arg1	activity					1362:1369	an improved antimicrobial activity	1336:1369	an improved antimicrobial activity against Porphyromonas gingivalis under high glucose concentration	1336:1435	What's more, it can sense the ambient glucose stimulus, rapidly and correspondingly adjust its inner pore structure to control the loaded metronidazole release, lead to an improved antimicrobial activity against Porphyromonas gingivalis under high glucose concentration.
30287380	5	8	theme	Cytotoxicity	763:774	arg1	test					776:779	Cytotoxicity test	763:779	Cytotoxicity test	763:779	Cytotoxicity test, drug release and anti-bacterial test were carried out, respectively.
30287380	0	9	theme	Porphyromonas	107:119	arg1	gingivalis					121:130	Porphyromonas gingivalis	107:130	Porphyromonas gingivalis proliferation	107:144	Glucose-sensitive delivery of metronidazole by using a photo-crosslinked chitosan hydrogel film to inhibit Porphyromonas gingivalis proliferation.
30287380	9	10	theme	sensitive	1451:1459	arg1	film					1470:1473	This glucose sensitive hydrogel film	1438:1473	This glucose sensitive hydrogel film	1438:1473	This glucose sensitive hydrogel film may provide a promising method for diabetic's periodontitis therapy in clinic.
30287380	8	11	dep	control	1286:1292	arg1	lead					1328:1331	lead	1328:1331	lead to an improved antimicrobial activity against Porphyromonas gingivalis under high glucose concentration	1328:1435	What's more, it can sense the ambient glucose stimulus, rapidly and correspondingly adjust its inner pore structure to control the loaded metronidazole release, lead to an improved antimicrobial activity against Porphyromonas gingivalis under high glucose concentration.
30287380	9	12	theme	hydrogel	1461:1468	arg1	film					1470:1473	This glucose sensitive hydrogel film	1438:1473	This glucose sensitive hydrogel film	1438:1473	This glucose sensitive hydrogel film may provide a promising method for diabetic's periodontitis therapy in clinic.
30287380	5	13	theme	drug	782:785	arg1	release					787:793	drug release	782:793	drug release	782:793	Cytotoxicity test, drug release and anti-bacterial test were carried out, respectively.
30287380	4	14	theme	mechanical	681:690	arg1	strength					692:699	mechanical strength	681:699	mechanical strength	681:699	The physicochemical characteristics including chemical composition, crosslinking degree, mechanical strength and related enzyme properties were investigated successively.
30287380	6	15	contain	has	910:912	arg2	properties					930:939	good mechanical properties	914:939	good mechanical properties	914:939	The results show that this photo-crosslinked hydrogel film has good mechanical properties; meanwhile, the immobilized enzyme's bonding capacity and activity can maintain a relatively high level after surface activation.
30287380	6	15	contain	has	910:912	arg1	film					905:908	this photo-crosslinked hydrogel film	873:908	this photo-crosslinked hydrogel film	873:908	The results show that this photo-crosslinked hydrogel film has good mechanical properties; meanwhile, the immobilized enzyme's bonding capacity and activity can maintain a relatively high level after surface activation.
30287380	3	16	theme	glucose	543:549	arg1	oxidase					551:557	glucose oxidase	543:557	glucose oxidase	543:557	Glucose sensitivity was endowed by immobilizing glucose oxidase on the surface of chitosan film.
30287380	1	17	theme	controlled	199:208	arg1	release					210:216	controlled release	199:216	controlled release of metronidazole	199:233	A novel glucose-sensitive drug delivery system with controlled release of metronidazole was synthesized by using biocompatible photo-crosslinked chitosan hydrogel film.
30287380	0	18	theme	gingivalis	121:130	arg1	proliferation					132:144	Porphyromonas gingivalis proliferation	107:144	Porphyromonas gingivalis proliferation	107:144	Glucose-sensitive delivery of metronidazole by using a photo-crosslinked chitosan hydrogel film to inhibit Porphyromonas gingivalis proliferation.
30287380	3	19	theme	Glucose	495:501	arg1	sensitivity					503:513	Glucose sensitivity	495:513	Glucose sensitivity	495:513	Glucose sensitivity was endowed by immobilizing glucose oxidase on the surface of chitosan film.
30287380	0	20	link	photo-crosslinked	55:71	arg1	film					91:94	a photo-crosslinked chitosan hydrogel film	53:94	a photo-crosslinked chitosan hydrogel film	53:94	Glucose-sensitive delivery of metronidazole by using a photo-crosslinked chitosan hydrogel film to inhibit Porphyromonas gingivalis proliferation.
30287380	2	21	theme	methacrylic	330:340	arg1	substance					371:379	photosensitive substance	356:379	photosensitive substance	356:379	Specifically, methacrylic anhydride, as photosensitive substance, was firstly grafted on chitosan molecular chains and then UV irradiation was applied as the crosslinking method.
30287380	2	21	theme	methacrylic	330:340	arg1	anhydride					342:350	methacrylic anhydride	330:350	methacrylic anhydride	330:350	Specifically, methacrylic anhydride, as photosensitive substance, was firstly grafted on chitosan molecular chains and then UV irradiation was applied as the crosslinking method.
30287380	8	22	theme	loaded	1298:1303	arg1	release					1319:1325	the loaded metronidazole release	1294:1325	the loaded metronidazole release	1294:1325	What's more, it can sense the ambient glucose stimulus, rapidly and correspondingly adjust its inner pore structure to control the loaded metronidazole release, lead to an improved antimicrobial activity against Porphyromonas gingivalis under high glucose concentration.
30287380	8	23	dep	adjust	1251:1256	arg1	sense					1187:1191	sense	1187:1191	sense	1187:1191	What's more, it can sense the ambient glucose stimulus, rapidly and correspondingly adjust its inner pore structure to control the loaded metronidazole release, lead to an improved antimicrobial activity against Porphyromonas gingivalis under high glucose concentration.
30287380	8	24	theme	inner	1262:1266	arg1	structure					1273:1281	its inner pore structure	1258:1281	its inner pore structure	1258:1281	What's more, it can sense the ambient glucose stimulus, rapidly and correspondingly adjust its inner pore structure to control the loaded metronidazole release, lead to an improved antimicrobial activity against Porphyromonas gingivalis under high glucose concentration.
30287380	0	25	theme	Glucose-sensitive	0:16	arg1	delivery					18:25	Glucose-sensitive delivery	0:25	Glucose-sensitive delivery of metronidazole by using a photo-crosslinked chitosan hydrogel film	0:94	Glucose-sensitive delivery of metronidazole by using a photo-crosslinked chitosan hydrogel film to inhibit Porphyromonas gingivalis proliferation.
30287380	7	26	theme	chemical	1137:1144	arg1	samples					1158:1164	chemical crosslinked samples	1137:1164	chemical crosslinked samples	1137:1164	In addition, this material possesses better biocompatibility than chemical crosslinked samples.
30287380	8	27	dep	sense	1187:1191	arg1	more					1174:1177	more	1174:1177	more	1174:1177	What's more, it can sense the ambient glucose stimulus, rapidly and correspondingly adjust its inner pore structure to control the loaded metronidazole release, lead to an improved antimicrobial activity against Porphyromonas gingivalis under high glucose concentration.
30287380	6	28	theme	good	914:917	arg1	properties					930:939	good mechanical properties	914:939	good mechanical properties	914:939	The results show that this photo-crosslinked hydrogel film has good mechanical properties; meanwhile, the immobilized enzyme's bonding capacity and activity can maintain a relatively high level after surface activation.
30287380	2	29	theme	UV	440:441	arg1	irradiation					443:453	then UV irradiation	435:453	then UV irradiation	435:453	Specifically, methacrylic anhydride, as photosensitive substance, was firstly grafted on chitosan molecular chains and then UV irradiation was applied as the crosslinking method.
30287380	2	29	theme	UV	440:441	arg1	method					487:492	the crosslinking method	470:492	the crosslinking method	470:492	Specifically, methacrylic anhydride, as photosensitive substance, was firstly grafted on chitosan molecular chains and then UV irradiation was applied as the crosslinking method.
30287380	0	30	theme	metronidazole	30:42	arg1	delivery					18:25	Glucose-sensitive delivery	0:25	Glucose-sensitive delivery of metronidazole by using a photo-crosslinked chitosan hydrogel film	0:94	Glucose-sensitive delivery of metronidazole by using a photo-crosslinked chitosan hydrogel film to inhibit Porphyromonas gingivalis proliferation.
30287380	4	31	theme	chemical	638:645	arg1	composition					647:657	chemical composition	638:657	chemical composition	638:657	The physicochemical characteristics including chemical composition, crosslinking degree, mechanical strength and related enzyme properties were investigated successively.
30287380	8	32	theme	glucose	1205:1211	arg1	stimulus					1213:1220	the ambient glucose stimulus	1193:1220	the ambient glucose stimulus	1193:1220	What's more, it can sense the ambient glucose stimulus, rapidly and correspondingly adjust its inner pore structure to control the loaded metronidazole release, lead to an improved antimicrobial activity against Porphyromonas gingivalis under high glucose concentration.
30287380	1	33	link	photo-crosslinked	274:290	arg1	film					310:313	biocompatible photo-crosslinked chitosan hydrogel film	260:313	biocompatible photo-crosslinked chitosan hydrogel film	260:313	A novel glucose-sensitive drug delivery system with controlled release of metronidazole was synthesized by using biocompatible photo-crosslinked chitosan hydrogel film.
30287380	8	34	theme	high	1410:1413	arg1	concentration					1423:1435	high glucose concentration	1410:1435	high glucose concentration	1410:1435	What's more, it can sense the ambient glucose stimulus, rapidly and correspondingly adjust its inner pore structure to control the loaded metronidazole release, lead to an improved antimicrobial activity against Porphyromonas gingivalis under high glucose concentration.
30287380	4	35	theme	crosslinking	660:671	arg1	degree					673:678	crosslinking degree	660:678	crosslinking degree	660:678	The physicochemical characteristics including chemical composition, crosslinking degree, mechanical strength and related enzyme properties were investigated successively.
30287380	4	36	theme	related	705:711	arg1	properties					720:729	related enzyme properties	705:729	related enzyme properties	705:729	The physicochemical characteristics including chemical composition, crosslinking degree, mechanical strength and related enzyme properties were investigated successively.
30287380	2	37	theme	photosensitive	356:369	arg1	substance					371:379	photosensitive substance	356:379	photosensitive substance	356:379	Specifically, methacrylic anhydride, as photosensitive substance, was firstly grafted on chitosan molecular chains and then UV irradiation was applied as the crosslinking method.
30287380	2	37	theme	photosensitive	356:369	arg1	anhydride					342:350	methacrylic anhydride	330:350	methacrylic anhydride	330:350	Specifically, methacrylic anhydride, as photosensitive substance, was firstly grafted on chitosan molecular chains and then UV irradiation was applied as the crosslinking method.
30287380	2	38	theme	crosslinking	474:485	arg1	irradiation					443:453	then UV irradiation	435:453	then UV irradiation	435:453	Specifically, methacrylic anhydride, as photosensitive substance, was firstly grafted on chitosan molecular chains and then UV irradiation was applied as the crosslinking method.
30287380	2	38	theme	crosslinking	474:485	arg1	method					487:492	the crosslinking method	470:492	the crosslinking method	470:492	Specifically, methacrylic anhydride, as photosensitive substance, was firstly grafted on chitosan molecular chains and then UV irradiation was applied as the crosslinking method.
30287380	8	39	theme	ambient	1197:1203	arg1	stimulus					1213:1220	the ambient glucose stimulus	1193:1220	the ambient glucose stimulus	1193:1220	What's more, it can sense the ambient glucose stimulus, rapidly and correspondingly adjust its inner pore structure to control the loaded metronidazole release, lead to an improved antimicrobial activity against Porphyromonas gingivalis under high glucose concentration.
30287380	1	40	theme	metronidazole	221:233	arg1	release					210:216	controlled release	199:216	controlled release of metronidazole	199:233	A novel glucose-sensitive drug delivery system with controlled release of metronidazole was synthesized by using biocompatible photo-crosslinked chitosan hydrogel film.
30287380	9	41	theme	promising	1489:1497	arg1	method					1499:1504	a promising method	1487:1504	a promising method for diabetic's periodontitis therapy in clinic	1487:1551	This glucose sensitive hydrogel film may provide a promising method for diabetic's periodontitis therapy in clinic.
30287380	4	42	theme	physicochemical	596:610	arg1	characteristics					612:626	The physicochemical characteristics	592:626	The physicochemical characteristics including chemical composition, crosslinking degree, mechanical strength and related enzyme properties	592:729	The physicochemical characteristics including chemical composition, crosslinking degree, mechanical strength and related enzyme properties were investigated successively.
30287380	4	42	theme	physicochemical	596:610	arg1	composition					647:657	chemical composition	638:657	chemical composition	638:657	The physicochemical characteristics including chemical composition, crosslinking degree, mechanical strength and related enzyme properties were investigated successively.
30287380	4	42	theme	physicochemical	596:610	arg1	degree					673:678	crosslinking degree	660:678	crosslinking degree	660:678	The physicochemical characteristics including chemical composition, crosslinking degree, mechanical strength and related enzyme properties were investigated successively.
30287380	4	42	theme	physicochemical	596:610	arg1	strength					692:699	mechanical strength	681:699	mechanical strength	681:699	The physicochemical characteristics including chemical composition, crosslinking degree, mechanical strength and related enzyme properties were investigated successively.
30287380	4	42	theme	physicochemical	596:610	arg1	properties					720:729	related enzyme properties	705:729	related enzyme properties	705:729	The physicochemical characteristics including chemical composition, crosslinking degree, mechanical strength and related enzyme properties were investigated successively.
30287380	6	43	theme	bonding	978:984	arg1	capacity					986:993	the immobilized enzyme's bonding capacity	953:993	the immobilized enzyme's bonding capacity	953:993	The results show that this photo-crosslinked hydrogel film has good mechanical properties; meanwhile, the immobilized enzyme's bonding capacity and activity can maintain a relatively high level after surface activation.
30287380	6	44	link	photo-crosslinked	878:894	arg1	film					905:908	this photo-crosslinked hydrogel film	873:908	this photo-crosslinked hydrogel film	873:908	The results show that this photo-crosslinked hydrogel film has good mechanical properties; meanwhile, the immobilized enzyme's bonding capacity and activity can maintain a relatively high level after surface activation.
30287380	6	45	theme	photo-crosslinked	878:894	arg1	film					905:908	this photo-crosslinked hydrogel film	873:908	this photo-crosslinked hydrogel film	873:908	The results show that this photo-crosslinked hydrogel film has good mechanical properties; meanwhile, the immobilized enzyme's bonding capacity and activity can maintain a relatively high level after surface activation.
30287380	8	46	theme	glucose	1415:1421	arg1	concentration					1423:1435	high glucose concentration	1410:1435	high glucose concentration	1410:1435	What's more, it can sense the ambient glucose stimulus, rapidly and correspondingly adjust its inner pore structure to control the loaded metronidazole release, lead to an improved antimicrobial activity against Porphyromonas gingivalis under high glucose concentration.
30287380	0	47	theme	photo-crosslinked	55:71	arg1	film					91:94	a photo-crosslinked chitosan hydrogel film	53:94	a photo-crosslinked chitosan hydrogel film	53:94	Glucose-sensitive delivery of metronidazole by using a photo-crosslinked chitosan hydrogel film to inhibit Porphyromonas gingivalis proliferation.
30287380	7	48	link	crosslinked	1146:1156	arg1	samples					1158:1164	chemical crosslinked samples	1137:1164	chemical crosslinked samples	1137:1164	In addition, this material possesses better biocompatibility than chemical crosslinked samples.
30287380	7	49	contain	possesses	1098:1106	arg2	biocompatibility					1115:1130	better biocompatibility	1108:1130	better biocompatibility than chemical crosslinked samples	1108:1164	In addition, this material possesses better biocompatibility than chemical crosslinked samples.
30287380	7	49	contain	possesses	1098:1106	arg1	material					1089:1096	this material	1084:1096	this material	1084:1096	In addition, this material possesses better biocompatibility than chemical crosslinked samples.
30287380	6	50	theme	hydrogel	896:903	arg1	film					905:908	this photo-crosslinked hydrogel film	873:908	this photo-crosslinked hydrogel film	873:908	The results show that this photo-crosslinked hydrogel film has good mechanical properties; meanwhile, the immobilized enzyme's bonding capacity and activity can maintain a relatively high level after surface activation.
30287380	2	51	theme	chitosan	405:412	arg1	chains					424:429	chitosan molecular chains	405:429	chitosan molecular chains	405:429	Specifically, methacrylic anhydride, as photosensitive substance, was firstly grafted on chitosan molecular chains and then UV irradiation was applied as the crosslinking method.
30287380	8	52	theme	improved	1339:1346	arg1	activity					1362:1369	an improved antimicrobial activity	1336:1369	an improved antimicrobial activity against Porphyromonas gingivalis under high glucose concentration	1336:1435	What's more, it can sense the ambient glucose stimulus, rapidly and correspondingly adjust its inner pore structure to control the loaded metronidazole release, lead to an improved antimicrobial activity against Porphyromonas gingivalis under high glucose concentration.
30287380	9	53	theme	periodontitis	1521:1533	arg1	therapy					1535:1541	diabetic's periodontitis therapy	1510:1541	diabetic's periodontitis therapy in clinic	1510:1551	This glucose sensitive hydrogel film may provide a promising method for diabetic's periodontitis therapy in clinic.
30287380	3	54	theme	chitosan	577:584	arg1	surface					566:572	the surface	562:572	the surface of chitosan film	562:589	Glucose sensitivity was endowed by immobilizing glucose oxidase on the surface of chitosan film.
30287380	6	55	theme	surface	1051:1057	arg1	activation					1059:1068	surface activation	1051:1068	surface activation	1051:1068	The results show that this photo-crosslinked hydrogel film has good mechanical properties; meanwhile, the immobilized enzyme's bonding capacity and activity can maintain a relatively high level after surface activation.
30287380	5	56	theme	anti-bacterial	799:812	arg1	test					814:817	anti-bacterial test	799:817	anti-bacterial test	799:817	Cytotoxicity test, drug release and anti-bacterial test were carried out, respectively.
30287380	6	57	theme	immobilized	957:967	arg1	capacity					986:993	the immobilized enzyme's bonding capacity	953:993	the immobilized enzyme's bonding capacity	953:993	The results show that this photo-crosslinked hydrogel film has good mechanical properties; meanwhile, the immobilized enzyme's bonding capacity and activity can maintain a relatively high level after surface activation.
30287380	7	58	theme	crosslinked	1146:1156	arg1	samples					1158:1164	chemical crosslinked samples	1137:1164	chemical crosslinked samples	1137:1164	In addition, this material possesses better biocompatibility than chemical crosslinked samples.
30287380	1	59	theme	novel	149:153	arg1	system					187:192	A novel glucose-sensitive drug delivery system	147:192	A novel glucose-sensitive drug delivery system with controlled release of metronidazole	147:233	A novel glucose-sensitive drug delivery system with controlled release of metronidazole was synthesized by using biocompatible photo-crosslinked chitosan hydrogel film.
30287380	1	60	theme	biocompatible	260:272	arg1	film					310:313	biocompatible photo-crosslinked chitosan hydrogel film	260:313	biocompatible photo-crosslinked chitosan hydrogel film	260:313	A novel glucose-sensitive drug delivery system with controlled release of metronidazole was synthesized by using biocompatible photo-crosslinked chitosan hydrogel film.
30287380	8	61	theme	metronidazole	1305:1317	arg1	release					1319:1325	the loaded metronidazole release	1294:1325	the loaded metronidazole release	1294:1325	What's more, it can sense the ambient glucose stimulus, rapidly and correspondingly adjust its inner pore structure to control the loaded metronidazole release, lead to an improved antimicrobial activity against Porphyromonas gingivalis under high glucose concentration.
30287380	1	62	theme	glucose-sensitive	155:171	arg1	system					187:192	A novel glucose-sensitive drug delivery system	147:192	A novel glucose-sensitive drug delivery system with controlled release of metronidazole	147:233	A novel glucose-sensitive drug delivery system with controlled release of metronidazole was synthesized by using biocompatible photo-crosslinked chitosan hydrogel film.
30287380	6	63	theme	mechanical	919:928	arg1	properties					930:939	good mechanical properties	914:939	good mechanical properties	914:939	The results show that this photo-crosslinked hydrogel film has good mechanical properties; meanwhile, the immobilized enzyme's bonding capacity and activity can maintain a relatively high level after surface activation.
30287380	1	64	theme	photo-crosslinked	274:290	arg1	film					310:313	biocompatible photo-crosslinked chitosan hydrogel film	260:313	biocompatible photo-crosslinked chitosan hydrogel film	260:313	A novel glucose-sensitive drug delivery system with controlled release of metronidazole was synthesized by using biocompatible photo-crosslinked chitosan hydrogel film.
30287380	0	65	theme	hydrogel	82:89	arg1	film					91:94	a photo-crosslinked chitosan hydrogel film	53:94	a photo-crosslinked chitosan hydrogel film	53:94	Glucose-sensitive delivery of metronidazole by using a photo-crosslinked chitosan hydrogel film to inhibit Porphyromonas gingivalis proliferation.
30287380	1	66	theme	drug	173:176	arg1	system					187:192	A novel glucose-sensitive drug delivery system	147:192	A novel glucose-sensitive drug delivery system with controlled release of metronidazole	147:233	A novel glucose-sensitive drug delivery system with controlled release of metronidazole was synthesized by using biocompatible photo-crosslinked chitosan hydrogel film.
30287380	1	67	theme	chitosan	292:299	arg1	film					310:313	biocompatible photo-crosslinked chitosan hydrogel film	260:313	biocompatible photo-crosslinked chitosan hydrogel film	260:313	A novel glucose-sensitive drug delivery system with controlled release of metronidazole was synthesized by using biocompatible photo-crosslinked chitosan hydrogel film.
30287380	0	68	theme	chitosan	73:80	arg1	film					91:94	a photo-crosslinked chitosan hydrogel film	53:94	a photo-crosslinked chitosan hydrogel film	53:94	Glucose-sensitive delivery of metronidazole by using a photo-crosslinked chitosan hydrogel film to inhibit Porphyromonas gingivalis proliferation.
30287380	9	69	theme	glucose	1443:1449	arg1	film					1470:1473	This glucose sensitive hydrogel film	1438:1473	This glucose sensitive hydrogel film	1438:1473	This glucose sensitive hydrogel film may provide a promising method for diabetic's periodontitis therapy in clinic.
30287380	4	70	theme	enzyme	713:718	arg1	properties					720:729	related enzyme properties	705:729	related enzyme properties	705:729	The physicochemical characteristics including chemical composition, crosslinking degree, mechanical strength and related enzyme properties were investigated successively.
30287380	1	71	theme	delivery	178:185	arg1	system					187:192	A novel glucose-sensitive drug delivery system	147:192	A novel glucose-sensitive drug delivery system with controlled release of metronidazole	147:233	A novel glucose-sensitive drug delivery system with controlled release of metronidazole was synthesized by using biocompatible photo-crosslinked chitosan hydrogel film.
30287380	1	72	theme	hydrogel	301:308	arg1	film					310:313	biocompatible photo-crosslinked chitosan hydrogel film	260:313	biocompatible photo-crosslinked chitosan hydrogel film	260:313	A novel glucose-sensitive drug delivery system with controlled release of metronidazole was synthesized by using biocompatible photo-crosslinked chitosan hydrogel film.
29533177	6	0	theme	pretoriensis	1162:1173	arg1	12673T					1179:1184	Amycolatopsis pretoriensis JCM 12673T	1148:1184	Amycolatopsis pretoriensis JCM 12673T (98.82 %)	1148:1194	Based on 16S rRNA gene sequence and phylogenetic analyses, strain C12CA1T was closely related to Amycolatopsis vancoresmycina DSM 44592T (98.96 %) and Amycolatopsis pretoriensis JCM 12673T (98.82 %).
29533177	6	0	theme	pretoriensis	1162:1173	arg1	%					1193:1193	98.82 %	1187:1193	98.82 %	1187:1193	Based on 16S rRNA gene sequence and phylogenetic analyses, strain C12CA1T was closely related to Amycolatopsis vancoresmycina DSM 44592T (98.96 %) and Amycolatopsis pretoriensis JCM 12673T (98.82 %).
29533177	3	1	theme	meso-2,6	298:305	arg1	arabinose					363:371	arabinose	363:371	arabinose	363:371	Strain C12CA1T contained meso-2,6 diaminopimelic acid in the cell-wall peptidoglycan, and arabinose and galactose as diagnostic sugars of the whole-cell hydrolysate.
29533177	3	1	theme	meso-2,6	298:305	arg1	acid					322:325	meso-2,6 diaminopimelic acid	298:325	meso-2,6 diaminopimelic acid in the cell-wall peptidoglycan	298:356	Strain C12CA1T contained meso-2,6 diaminopimelic acid in the cell-wall peptidoglycan, and arabinose and galactose as diagnostic sugars of the whole-cell hydrolysate.
29533177	3	1	theme	meso-2,6	298:305	arg1	galactose					377:385	galactose	377:385	galactose	377:385	Strain C12CA1T contained meso-2,6 diaminopimelic acid in the cell-wall peptidoglycan, and arabinose and galactose as diagnostic sugars of the whole-cell hydrolysate.
29533177	3	1	theme	meso-2,6	298:305	arg1	sugars					401:406	diagnostic sugars	390:406	diagnostic sugars of the whole-cell hydrolysate	390:436	Strain C12CA1T contained meso-2,6 diaminopimelic acid in the cell-wall peptidoglycan, and arabinose and galactose as diagnostic sugars of the whole-cell hydrolysate.
29533177	5	2	theme	unidentified	947:958	arg1	phospholipid					983:994	an unidentified glucosamine-containing phospholipid	944:994	an unidentified glucosamine-containing phospholipid	944:994	It contained MK-9(H4) as the predominant menaquinone, C16 : 0, iso-C15 : 0 and iso-C16 : 0 as the major cellular fatty acids, and several phospholipids consisting of diphosphotidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol, hydroxyphosphatidylethanolamine, phosphatidylinositol mannosides and an unidentified glucosamine-containing phospholipid.
29533177	9	3	theme	strain	1462:1467	arg1	C12CA1T					1469:1475	strain C12CA1T	1462:1475	strain C12CA1T	1462:1475	Based on the results of polyphasic characterization, strain C12CA1T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsis silviterrae sp.
29533177	3	4	theme	cell-wall	334:342	arg1	peptidoglycan					344:356	the cell-wall peptidoglycan	330:356	the cell-wall peptidoglycan	330:356	Strain C12CA1T contained meso-2,6 diaminopimelic acid in the cell-wall peptidoglycan, and arabinose and galactose as diagnostic sugars of the whole-cell hydrolysate.
29533177	4	5	theme	strain	505:510	arg1	C12CA1T					512:518	strain C12CA1T	505:518	strain C12CA1T	505:518	On the basis of morphological and chemotaxonomic characteristics, strain C12CA1T was classified in the genus Amycolatopsis.
29533177	2	6	theme	polyphasic	252:261	arg1	approach					263:270	a polyphasic approach	250:270	a polyphasic approach	250:270	A novel actinobacterial strain, designated C12CA1T, was isolated from forest soil in the conservation area of Chulabhorn dam, Thailand, and its taxonomic position was determined by using a polyphasic approach.
29533177	6	7	theme	strain	1056:1061	arg1	C12CA1T					1063:1069	strain C12CA1T	1056:1069	strain C12CA1T	1056:1069	Based on 16S rRNA gene sequence and phylogenetic analyses, strain C12CA1T was closely related to Amycolatopsis vancoresmycina DSM 44592T (98.96 %) and Amycolatopsis pretoriensis JCM 12673T (98.82 %).
29533177	5	8	theme	fatty	676:680	arg1	acids					682:686	the major cellular fatty acids	657:686	the major cellular fatty acids	657:686	It contained MK-9(H4) as the predominant menaquinone, C16 : 0, iso-C15 : 0 and iso-C16 : 0 as the major cellular fatty acids, and several phospholipids consisting of diphosphotidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol, hydroxyphosphatidylethanolamine, phosphatidylinositol mannosides and an unidentified glucosamine-containing phospholipid.
29533177	4	9	theme	morphological	455:467	arg1	characteristics					488:502	morphological and chemotaxonomic characteristics	455:502	morphological and chemotaxonomic characteristics	455:502	On the basis of morphological and chemotaxonomic characteristics, strain C12CA1T was classified in the genus Amycolatopsis.
29533177	8	10	theme	C12CA1T	1386:1392	arg1	G+C content					1364:1374	The DNA G+C content	1356:1374	The DNA G+C content of strain C12CA1T	1356:1392	The DNA G+C content of strain C12CA1T was 69.8 mol%.
29533177	8	10	theme	C12CA1T	1386:1392	arg1	%					1406:1406	69.8 mol%	1398:1406	69.8 mol%	1398:1406	The DNA G+C content of strain C12CA1T was 69.8 mol%.
29533177	6	11	theme	16S	1006:1008	arg1	rRNA					1010:1013	16S rRNA	1006:1013	16S rRNA gene sequence	1006:1027	Based on 16S rRNA gene sequence and phylogenetic analyses, strain C12CA1T was closely related to Amycolatopsis vancoresmycina DSM 44592T (98.96 %) and Amycolatopsis pretoriensis JCM 12673T (98.82 %).
29533177	8	12	theme	strain	1379:1384	arg1	C12CA1T					1386:1392	strain C12CA1T	1379:1392	strain C12CA1T	1379:1392	The DNA G+C content of strain C12CA1T was 69.8 mol%.
29533177	9	13	theme	name	1546:1549	arg1	sp					1577:1578	the name Amycolatopsis silviterrae sp	1542:1578	the name Amycolatopsis silviterrae sp	1542:1578	Based on the results of polyphasic characterization, strain C12CA1T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsis silviterrae sp.
29533177	2	14	theme	forest	133:138	arg1	soil					140:143	forest soil	133:143	forest soil in the conservation area of Chulabhorn dam, Thailand	133:196	A novel actinobacterial strain, designated C12CA1T, was isolated from forest soil in the conservation area of Chulabhorn dam, Thailand, and its taxonomic position was determined by using a polyphasic approach.
29533177	5	15	theme	predominant	592:602	arg1	menaquinone					604:614	the predominant menaquinone	588:614	the predominant menaquinone	588:614	It contained MK-9(H4) as the predominant menaquinone, C16 : 0, iso-C15 : 0 and iso-C16 : 0 as the major cellular fatty acids, and several phospholipids consisting of diphosphotidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol, hydroxyphosphatidylethanolamine, phosphatidylinositol mannosides and an unidentified glucosamine-containing phospholipid.
29533177	5	15	theme	predominant	592:602	arg1	MK-9					576:579	MK-9	576:579	MK-9(H4)	576:583	It contained MK-9(H4) as the predominant menaquinone, C16 : 0, iso-C15 : 0 and iso-C16 : 0 as the major cellular fatty acids, and several phospholipids consisting of diphosphotidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol, hydroxyphosphatidylethanolamine, phosphatidylinositol mannosides and an unidentified glucosamine-containing phospholipid.
29533177	11	16	theme	1456T=NBRC	1633:1642	arg1	C12CA1T					1618:1624	C12CA1T	1618:1624	C12CA1T (=TBRC 1456T=NBRC 111116T)	1618:1651	The type strain is C12CA1T (=TBRC 1456T=NBRC 111116T).
29533177	11	16	theme	1456T=NBRC	1633:1642	arg1	111116T					1644:1650	=TBRC 1456T=NBRC 111116T	1627:1650	=TBRC 1456T=NBRC 111116T	1627:1650	The type strain is C12CA1T (=TBRC 1456T=NBRC 111116T).
29533177	3	17	theme	whole-cell	415:424	arg1	hydrolysate					426:436	the whole-cell hydrolysate	411:436	the whole-cell hydrolysate	411:436	Strain C12CA1T contained meso-2,6 diaminopimelic acid in the cell-wall peptidoglycan, and arabinose and galactose as diagnostic sugars of the whole-cell hydrolysate.
29533177	6	18	theme	vancoresmycina	1108:1121	arg1	44592T					1127:1132	Amycolatopsis vancoresmycina DSM 44592T	1094:1132	Amycolatopsis vancoresmycina DSM 44592T (98.96 %)	1094:1142	Based on 16S rRNA gene sequence and phylogenetic analyses, strain C12CA1T was closely related to Amycolatopsis vancoresmycina DSM 44592T (98.96 %) and Amycolatopsis pretoriensis JCM 12673T (98.82 %).
29533177	6	18	theme	vancoresmycina	1108:1121	arg1	%					1141:1141	98.96 %	1135:1141	98.96 %	1135:1141	Based on 16S rRNA gene sequence and phylogenetic analyses, strain C12CA1T was closely related to Amycolatopsis vancoresmycina DSM 44592T (98.96 %) and Amycolatopsis pretoriensis JCM 12673T (98.82 %).
29533177	3	19	from	arabinose	363:371	arg1	peptidoglycan					344:356	the cell-wall peptidoglycan	330:356	the cell-wall peptidoglycan	330:356	Strain C12CA1T contained meso-2,6 diaminopimelic acid in the cell-wall peptidoglycan, and arabinose and galactose as diagnostic sugars of the whole-cell hydrolysate.
29533177	5	20	dep	C16 	617:620	arg1	iso-C16 					642:649	iso-C16 	642:649	iso-C16 	642:649	It contained MK-9(H4) as the predominant menaquinone, C16 : 0, iso-C15 : 0 and iso-C16 : 0 as the major cellular fatty acids, and several phospholipids consisting of diphosphotidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol, hydroxyphosphatidylethanolamine, phosphatidylinositol mannosides and an unidentified glucosamine-containing phospholipid.
29533177	5	20	dep	C16 	617:620	arg1	 0					635:636	 0	635:636	 0	635:636	It contained MK-9(H4) as the predominant menaquinone, C16 : 0, iso-C15 : 0 and iso-C16 : 0 as the major cellular fatty acids, and several phospholipids consisting of diphosphotidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol, hydroxyphosphatidylethanolamine, phosphatidylinositol mannosides and an unidentified glucosamine-containing phospholipid.
29533177	5	20	dep	C16 	617:620	arg1	 0					651:652	 0	651:652	 0	651:652	It contained MK-9(H4) as the predominant menaquinone, C16 : 0, iso-C15 : 0 and iso-C16 : 0 as the major cellular fatty acids, and several phospholipids consisting of diphosphotidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol, hydroxyphosphatidylethanolamine, phosphatidylinositol mannosides and an unidentified glucosamine-containing phospholipid.
29533177	8	21	theme	69.8 mol	1398:1405	arg1	G+C content					1364:1374	The DNA G+C content	1356:1374	The DNA G+C content of strain C12CA1T	1356:1392	The DNA G+C content of strain C12CA1T was 69.8 mol%.
29533177	8	21	theme	69.8 mol	1398:1405	arg1	%					1406:1406	69.8 mol%	1398:1406	69.8 mol%	1398:1406	The DNA G+C content of strain C12CA1T was 69.8 mol%.
29533177	3	22	theme	hydrolysate	426:436	arg1	arabinose					363:371	arabinose	363:371	arabinose	363:371	Strain C12CA1T contained meso-2,6 diaminopimelic acid in the cell-wall peptidoglycan, and arabinose and galactose as diagnostic sugars of the whole-cell hydrolysate.
29533177	3	22	theme	hydrolysate	426:436	arg1	acid					322:325	meso-2,6 diaminopimelic acid	298:325	meso-2,6 diaminopimelic acid in the cell-wall peptidoglycan	298:356	Strain C12CA1T contained meso-2,6 diaminopimelic acid in the cell-wall peptidoglycan, and arabinose and galactose as diagnostic sugars of the whole-cell hydrolysate.
29533177	3	22	theme	hydrolysate	426:436	arg1	galactose					377:385	galactose	377:385	galactose	377:385	Strain C12CA1T contained meso-2,6 diaminopimelic acid in the cell-wall peptidoglycan, and arabinose and galactose as diagnostic sugars of the whole-cell hydrolysate.
29533177	3	22	theme	hydrolysate	426:436	arg1	sugars					401:406	diagnostic sugars	390:406	diagnostic sugars of the whole-cell hydrolysate	390:436	Strain C12CA1T contained meso-2,6 diaminopimelic acid in the cell-wall peptidoglycan, and arabinose and galactose as diagnostic sugars of the whole-cell hydrolysate.
29533177	0	23	theme	silviterrae	14:24	arg1	sp					26:27	Amycolatopsis silviterrae sp	0:27	Amycolatopsis silviterrae sp.	0:28	Amycolatopsis silviterrae sp.
29533177	7	24	theme	relatedness	1230:1240	arg1	values					1242:1247	low DNA-DNA relatedness values	1218:1247	low DNA-DNA relatedness values	1218:1247	The strain exhibited low DNA-DNA relatedness values with A. vancoresmycina DSM 44592T (6.9±0.2-11.6±1.9 %) and A. pretoriensis JCM 12673T (8.8±0.3-9.2±1.8 %).
29533177	9	25	theme	genus	1511:1515	arg1	Amycolatopsis					1517:1529	the genus Amycolatopsis	1507:1529	the genus Amycolatopsis	1507:1529	Based on the results of polyphasic characterization, strain C12CA1T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsis silviterrae sp.
29533177	0	26	theme	Amycolatopsis	0:12	arg1	sp					26:27	Amycolatopsis silviterrae sp	0:27	Amycolatopsis silviterrae sp.	0:28	Amycolatopsis silviterrae sp.
29533177	5	27	theme	phosphatidylinositol	908:927	arg1	mannosides					929:938	phosphatidylinositol mannosides	908:938	phosphatidylinositol mannosides	908:938	It contained MK-9(H4) as the predominant menaquinone, C16 : 0, iso-C15 : 0 and iso-C16 : 0 as the major cellular fatty acids, and several phospholipids consisting of diphosphotidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol, hydroxyphosphatidylethanolamine, phosphatidylinositol mannosides and an unidentified glucosamine-containing phospholipid.
29533177	7	28	theme	low	1218:1220	arg1	values					1242:1247	low DNA-DNA relatedness values	1218:1247	low DNA-DNA relatedness values	1218:1247	The strain exhibited low DNA-DNA relatedness values with A. vancoresmycina DSM 44592T (6.9±0.2-11.6±1.9 %) and A. pretoriensis JCM 12673T (8.8±0.3-9.2±1.8 %).
29533177	4	29	theme	genus	542:546	arg1	Amycolatopsis					548:560	the genus Amycolatopsis	538:560	the genus Amycolatopsis	538:560	On the basis of morphological and chemotaxonomic characteristics, strain C12CA1T was classified in the genus Amycolatopsis.
29533177	11	30	theme	type	1603:1606	arg1	C12CA1T					1618:1624	C12CA1T	1618:1624	C12CA1T (=TBRC 1456T=NBRC 111116T)	1618:1651	The type strain is C12CA1T (=TBRC 1456T=NBRC 111116T).
29533177	11	30	theme	type	1603:1606	arg1	strain					1608:1613	The type strain	1599:1613	The type strain	1599:1613	The type strain is C12CA1T (=TBRC 1456T=NBRC 111116T).
29533177	7	31	dep	A.	1308:1309	arg1	pretoriensis					1311:1322	pretoriensis	1311:1322	pretoriensis	1311:1322	The strain exhibited low DNA-DNA relatedness values with A. vancoresmycina DSM 44592T (6.9±0.2-11.6±1.9 %) and A. pretoriensis JCM 12673T (8.8±0.3-9.2±1.8 %).
29533177	7	32	dep	A.	1254:1255	arg1	vancoresmycina					1257:1270	vancoresmycina	1257:1270	vancoresmycina	1257:1270	The strain exhibited low DNA-DNA relatedness values with A. vancoresmycina DSM 44592T (6.9±0.2-11.6±1.9 %) and A. pretoriensis JCM 12673T (8.8±0.3-9.2±1.8 %).
29533177	7	33	theme	DNA-DNA	1222:1228	arg1	values					1242:1247	low DNA-DNA relatedness values	1218:1247	low DNA-DNA relatedness values	1218:1247	The strain exhibited low DNA-DNA relatedness values with A. vancoresmycina DSM 44592T (6.9±0.2-11.6±1.9 %) and A. pretoriensis JCM 12673T (8.8±0.3-9.2±1.8 %).
29533177	6	34	theme	Amycolatopsis	1148:1160	arg1	12673T					1179:1184	Amycolatopsis pretoriensis JCM 12673T	1148:1184	Amycolatopsis pretoriensis JCM 12673T (98.82 %)	1148:1194	Based on 16S rRNA gene sequence and phylogenetic analyses, strain C12CA1T was closely related to Amycolatopsis vancoresmycina DSM 44592T (98.96 %) and Amycolatopsis pretoriensis JCM 12673T (98.82 %).
29533177	6	34	theme	Amycolatopsis	1148:1160	arg1	%					1193:1193	98.82 %	1187:1193	98.82 %	1187:1193	Based on 16S rRNA gene sequence and phylogenetic analyses, strain C12CA1T was closely related to Amycolatopsis vancoresmycina DSM 44592T (98.96 %) and Amycolatopsis pretoriensis JCM 12673T (98.82 %).
29533177	9	35	theme	Amycolatopsis	1551:1563	arg1	sp					1577:1578	the name Amycolatopsis silviterrae sp	1542:1578	the name Amycolatopsis silviterrae sp	1542:1578	Based on the results of polyphasic characterization, strain C12CA1T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsis silviterrae sp.
29533177	3	36	theme	diagnostic	390:399	arg1	arabinose					363:371	arabinose	363:371	arabinose	363:371	Strain C12CA1T contained meso-2,6 diaminopimelic acid in the cell-wall peptidoglycan, and arabinose and galactose as diagnostic sugars of the whole-cell hydrolysate.
29533177	3	36	theme	diagnostic	390:399	arg1	acid					322:325	meso-2,6 diaminopimelic acid	298:325	meso-2,6 diaminopimelic acid in the cell-wall peptidoglycan	298:356	Strain C12CA1T contained meso-2,6 diaminopimelic acid in the cell-wall peptidoglycan, and arabinose and galactose as diagnostic sugars of the whole-cell hydrolysate.
29533177	3	36	theme	diagnostic	390:399	arg1	galactose					377:385	galactose	377:385	galactose	377:385	Strain C12CA1T contained meso-2,6 diaminopimelic acid in the cell-wall peptidoglycan, and arabinose and galactose as diagnostic sugars of the whole-cell hydrolysate.
29533177	3	36	theme	diagnostic	390:399	arg1	sugars					401:406	diagnostic sugars	390:406	diagnostic sugars of the whole-cell hydrolysate	390:436	Strain C12CA1T contained meso-2,6 diaminopimelic acid in the cell-wall peptidoglycan, and arabinose and galactose as diagnostic sugars of the whole-cell hydrolysate.
29533177	2	37	theme	conservation	152:163	arg1	area					165:168	the conservation area	148:168	the conservation area of Chulabhorn dam, Thailand	148:196	A novel actinobacterial strain, designated C12CA1T, was isolated from forest soil in the conservation area of Chulabhorn dam, Thailand, and its taxonomic position was determined by using a polyphasic approach.
29533177	6	38	theme	DSM	1123:1125	arg1	44592T					1127:1132	Amycolatopsis vancoresmycina DSM 44592T	1094:1132	Amycolatopsis vancoresmycina DSM 44592T (98.96 %)	1094:1142	Based on 16S rRNA gene sequence and phylogenetic analyses, strain C12CA1T was closely related to Amycolatopsis vancoresmycina DSM 44592T (98.96 %) and Amycolatopsis pretoriensis JCM 12673T (98.82 %).
29533177	6	38	theme	DSM	1123:1125	arg1	%					1141:1141	98.96 %	1135:1141	98.96 %	1135:1141	Based on 16S rRNA gene sequence and phylogenetic analyses, strain C12CA1T was closely related to Amycolatopsis vancoresmycina DSM 44592T (98.96 %) and Amycolatopsis pretoriensis JCM 12673T (98.82 %).
29533177	6	39	theme	gene	1015:1018	arg1	sequence					1020:1027	16S rRNA gene sequence	1006:1027	16S rRNA gene sequence	1006:1027	Based on 16S rRNA gene sequence and phylogenetic analyses, strain C12CA1T was closely related to Amycolatopsis vancoresmycina DSM 44592T (98.96 %) and Amycolatopsis pretoriensis JCM 12673T (98.82 %).
29533177	5	40	theme	major	661:665	arg1	acids					682:686	the major cellular fatty acids	657:686	the major cellular fatty acids	657:686	It contained MK-9(H4) as the predominant menaquinone, C16 : 0, iso-C15 : 0 and iso-C16 : 0 as the major cellular fatty acids, and several phospholipids consisting of diphosphotidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol, hydroxyphosphatidylethanolamine, phosphatidylinositol mannosides and an unidentified glucosamine-containing phospholipid.
29533177	11	41	theme	=TBRC	1627:1631	arg1	C12CA1T					1618:1624	C12CA1T	1618:1624	C12CA1T (=TBRC 1456T=NBRC 111116T)	1618:1651	The type strain is C12CA1T (=TBRC 1456T=NBRC 111116T).
29533177	11	41	theme	=TBRC	1627:1631	arg1	111116T					1644:1650	=TBRC 1456T=NBRC 111116T	1627:1650	=TBRC 1456T=NBRC 111116T	1627:1650	The type strain is C12CA1T (=TBRC 1456T=NBRC 111116T).
29533177	4	42	dep	characteristics	488:502	arg1	the					442:444	the	442:444	the	442:444	On the basis of morphological and chemotaxonomic characteristics, strain C12CA1T was classified in the genus Amycolatopsis.
29533177	4	42	dep	characteristics	488:502	arg1	basis					446:450	basis	446:450	basis	446:450	On the basis of morphological and chemotaxonomic characteristics, strain C12CA1T was classified in the genus Amycolatopsis.
29533177	2	43	from	soil	140:143	arg1	area					165:168	the conservation area	148:168	the conservation area of Chulabhorn dam, Thailand	148:196	A novel actinobacterial strain, designated C12CA1T, was isolated from forest soil in the conservation area of Chulabhorn dam, Thailand, and its taxonomic position was determined by using a polyphasic approach.
29533177	5	44	theme	cellular	667:674	arg1	acids					682:686	the major cellular fatty acids	657:686	the major cellular fatty acids	657:686	It contained MK-9(H4) as the predominant menaquinone, C16 : 0, iso-C15 : 0 and iso-C16 : 0 as the major cellular fatty acids, and several phospholipids consisting of diphosphotidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol, hydroxyphosphatidylethanolamine, phosphatidylinositol mannosides and an unidentified glucosamine-containing phospholipid.
29533177	3	45	theme	Strain	273:278	arg1	C12CA1T					280:286	Strain C12CA1T	273:286	Strain C12CA1T	273:286	Strain C12CA1T contained meso-2,6 diaminopimelic acid in the cell-wall peptidoglycan, and arabinose and galactose as diagnostic sugars of the whole-cell hydrolysate.
29533177	6	46	theme	phylogenetic	1033:1044	arg1	analyses					1046:1053	phylogenetic analyses	1033:1053	phylogenetic analyses	1033:1053	Based on 16S rRNA gene sequence and phylogenetic analyses, strain C12CA1T was closely related to Amycolatopsis vancoresmycina DSM 44592T (98.96 %) and Amycolatopsis pretoriensis JCM 12673T (98.82 %).
29533177	9	47	theme	Amycolatopsis	1517:1529	arg1	species					1496:1502	a novel species	1488:1502	a novel species	1488:1502	Based on the results of polyphasic characterization, strain C12CA1T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsis silviterrae sp.
29533177	4	48	theme	chemotaxonomic	473:486	arg1	characteristics					488:502	morphological and chemotaxonomic characteristics	455:502	morphological and chemotaxonomic characteristics	455:502	On the basis of morphological and chemotaxonomic characteristics, strain C12CA1T was classified in the genus Amycolatopsis.
29533177	5	49	contain	contained	566:574	arg2	menaquinone					604:614	the predominant menaquinone	588:614	the predominant menaquinone	588:614	It contained MK-9(H4) as the predominant menaquinone, C16 : 0, iso-C15 : 0 and iso-C16 : 0 as the major cellular fatty acids, and several phospholipids consisting of diphosphotidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol, hydroxyphosphatidylethanolamine, phosphatidylinositol mannosides and an unidentified glucosamine-containing phospholipid.
29533177	5	49	contain	contained	566:574	arg2	phospholipids					701:713	several phospholipids	693:713	several phospholipids consisting of diphosphotidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol, hydroxyphosphatidylethanolamine, phosphatidylinositol mannosides and an unidentified glucosamine-containing phospholipid	693:994	It contained MK-9(H4) as the predominant menaquinone, C16 : 0, iso-C15 : 0 and iso-C16 : 0 as the major cellular fatty acids, and several phospholipids consisting of diphosphotidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol, hydroxyphosphatidylethanolamine, phosphatidylinositol mannosides and an unidentified glucosamine-containing phospholipid.
29533177	5	49	contain	contained	566:574	arg2	H4					581:582	H4	581:582	H4	581:582	It contained MK-9(H4) as the predominant menaquinone, C16 : 0, iso-C15 : 0 and iso-C16 : 0 as the major cellular fatty acids, and several phospholipids consisting of diphosphotidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol, hydroxyphosphatidylethanolamine, phosphatidylinositol mannosides and an unidentified glucosamine-containing phospholipid.
29533177	5	49	contain	contained	566:574	arg2	MK-9					576:579	MK-9	576:579	MK-9(H4)	576:583	It contained MK-9(H4) as the predominant menaquinone, C16 : 0, iso-C15 : 0 and iso-C16 : 0 as the major cellular fatty acids, and several phospholipids consisting of diphosphotidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol, hydroxyphosphatidylethanolamine, phosphatidylinositol mannosides and an unidentified glucosamine-containing phospholipid.
29533177	5	49	contain	contained	566:574	arg1	It					563:564	It	563:564	It	563:564	It contained MK-9(H4) as the predominant menaquinone, C16 : 0, iso-C15 : 0 and iso-C16 : 0 as the major cellular fatty acids, and several phospholipids consisting of diphosphotidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol, hydroxyphosphatidylethanolamine, phosphatidylinositol mannosides and an unidentified glucosamine-containing phospholipid.
29533177	2	50	theme	actinobacterial	71:85	arg1	strain					87:92	A novel actinobacterial strain	63:92	A novel actinobacterial strain	63:92	A novel actinobacterial strain, designated C12CA1T, was isolated from forest soil in the conservation area of Chulabhorn dam, Thailand, and its taxonomic position was determined by using a polyphasic approach.
29533177	7	51	theme	A.	1254:1255	arg1	44592T					1276:1281	A. vancoresmycina DSM 44592T	1254:1281	A. vancoresmycina DSM 44592T (6.9±0.2-11.6±1.9 %)	1254:1302	The strain exhibited low DNA-DNA relatedness values with A. vancoresmycina DSM 44592T (6.9±0.2-11.6±1.9 %) and A. pretoriensis JCM 12673T (8.8±0.3-9.2±1.8 %).
29533177	7	51	theme	A.	1254:1255	arg1	%					1301:1301	6.9±0.2-11.6±1.9 %	1284:1301	6.9±0.2-11.6±1.9 %	1284:1301	The strain exhibited low DNA-DNA relatedness values with A. vancoresmycina DSM 44592T (6.9±0.2-11.6±1.9 %) and A. pretoriensis JCM 12673T (8.8±0.3-9.2±1.8 %).
29533177	2	52	theme	Thailand	189:196	arg1	area					165:168	the conservation area	148:168	the conservation area of Chulabhorn dam, Thailand	148:196	A novel actinobacterial strain, designated C12CA1T, was isolated from forest soil in the conservation area of Chulabhorn dam, Thailand, and its taxonomic position was determined by using a polyphasic approach.
29533177	7	53	theme	DSM	1272:1274	arg1	44592T					1276:1281	A. vancoresmycina DSM 44592T	1254:1281	A. vancoresmycina DSM 44592T (6.9±0.2-11.6±1.9 %)	1254:1302	The strain exhibited low DNA-DNA relatedness values with A. vancoresmycina DSM 44592T (6.9±0.2-11.6±1.9 %) and A. pretoriensis JCM 12673T (8.8±0.3-9.2±1.8 %).
29533177	7	53	theme	DSM	1272:1274	arg1	%					1301:1301	6.9±0.2-11.6±1.9 %	1284:1301	6.9±0.2-11.6±1.9 %	1284:1301	The strain exhibited low DNA-DNA relatedness values with A. vancoresmycina DSM 44592T (6.9±0.2-11.6±1.9 %) and A. pretoriensis JCM 12673T (8.8±0.3-9.2±1.8 %).
29533177	2	54	theme	novel	65:69	arg1	strain					87:92	A novel actinobacterial strain	63:92	A novel actinobacterial strain	63:92	A novel actinobacterial strain, designated C12CA1T, was isolated from forest soil in the conservation area of Chulabhorn dam, Thailand, and its taxonomic position was determined by using a polyphasic approach.
29533177	9	55	theme	silviterrae	1565:1575	arg1	sp					1577:1578	the name Amycolatopsis silviterrae sp	1542:1578	the name Amycolatopsis silviterrae sp	1542:1578	Based on the results of polyphasic characterization, strain C12CA1T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsis silviterrae sp.
29533177	2	56	attach	isolated	119:126	arg2	strain					87:92	A novel actinobacterial strain	63:92	A novel actinobacterial strain	63:92	A novel actinobacterial strain, designated C12CA1T, was isolated from forest soil in the conservation area of Chulabhorn dam, Thailand, and its taxonomic position was determined by using a polyphasic approach.
29533177	2	56	attach	isolated	119:126	arg1	soil					140:143	forest soil	133:143	forest soil in the conservation area of Chulabhorn dam, Thailand	133:196	A novel actinobacterial strain, designated C12CA1T, was isolated from forest soil in the conservation area of Chulabhorn dam, Thailand, and its taxonomic position was determined by using a polyphasic approach.
29533177	6	57	theme	Amycolatopsis	1094:1106	arg1	44592T					1127:1132	Amycolatopsis vancoresmycina DSM 44592T	1094:1132	Amycolatopsis vancoresmycina DSM 44592T (98.96 %)	1094:1142	Based on 16S rRNA gene sequence and phylogenetic analyses, strain C12CA1T was closely related to Amycolatopsis vancoresmycina DSM 44592T (98.96 %) and Amycolatopsis pretoriensis JCM 12673T (98.82 %).
29533177	6	57	theme	Amycolatopsis	1094:1106	arg1	%					1141:1141	98.96 %	1135:1141	98.96 %	1135:1141	Based on 16S rRNA gene sequence and phylogenetic analyses, strain C12CA1T was closely related to Amycolatopsis vancoresmycina DSM 44592T (98.96 %) and Amycolatopsis pretoriensis JCM 12673T (98.82 %).
29533177	5	58	theme	glucosamine-containing	960:981	arg1	phospholipid					983:994	an unidentified glucosamine-containing phospholipid	944:994	an unidentified glucosamine-containing phospholipid	944:994	It contained MK-9(H4) as the predominant menaquinone, C16 : 0, iso-C15 : 0 and iso-C16 : 0 as the major cellular fatty acids, and several phospholipids consisting of diphosphotidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol, hydroxyphosphatidylethanolamine, phosphatidylinositol mannosides and an unidentified glucosamine-containing phospholipid.
29533177	1	59	theme	forest	50:55	arg1	soil					57:60	forest soil	50:60	forest soil	50:60	nov., isolated from forest soil.
29533177	8	60	theme	DNA	1360:1362	arg1	G+C content					1364:1374	The DNA G+C content	1356:1374	The DNA G+C content of strain C12CA1T	1356:1392	The DNA G+C content of strain C12CA1T was 69.8 mol%.
29533177	8	60	theme	DNA	1360:1362	arg1	%					1406:1406	69.8 mol%	1398:1406	69.8 mol%	1398:1406	The DNA G+C content of strain C12CA1T was 69.8 mol%.
29533177	7	61	theme	JCM	1324:1326	arg1	12673T					1328:1333	A. pretoriensis JCM 12673T	1308:1333	A. pretoriensis JCM 12673T (8.8±0.3-9.2±1.8 %)	1308:1353	The strain exhibited low DNA-DNA relatedness values with A. vancoresmycina DSM 44592T (6.9±0.2-11.6±1.9 %) and A. pretoriensis JCM 12673T (8.8±0.3-9.2±1.8 %).
29533177	7	61	theme	JCM	1324:1326	arg1	%					1352:1352	8.8±0.3-9.2±1.8 %	1336:1352	8.8±0.3-9.2±1.8 %	1336:1352	The strain exhibited low DNA-DNA relatedness values with A. vancoresmycina DSM 44592T (6.9±0.2-11.6±1.9 %) and A. pretoriensis JCM 12673T (8.8±0.3-9.2±1.8 %).
29533177	3	62	theme	diaminopimelic	307:320	arg1	arabinose					363:371	arabinose	363:371	arabinose	363:371	Strain C12CA1T contained meso-2,6 diaminopimelic acid in the cell-wall peptidoglycan, and arabinose and galactose as diagnostic sugars of the whole-cell hydrolysate.
29533177	3	62	theme	diaminopimelic	307:320	arg1	acid					322:325	meso-2,6 diaminopimelic acid	298:325	meso-2,6 diaminopimelic acid in the cell-wall peptidoglycan	298:356	Strain C12CA1T contained meso-2,6 diaminopimelic acid in the cell-wall peptidoglycan, and arabinose and galactose as diagnostic sugars of the whole-cell hydrolysate.
29533177	3	62	theme	diaminopimelic	307:320	arg1	galactose					377:385	galactose	377:385	galactose	377:385	Strain C12CA1T contained meso-2,6 diaminopimelic acid in the cell-wall peptidoglycan, and arabinose and galactose as diagnostic sugars of the whole-cell hydrolysate.
29533177	3	62	theme	diaminopimelic	307:320	arg1	sugars					401:406	diagnostic sugars	390:406	diagnostic sugars of the whole-cell hydrolysate	390:436	Strain C12CA1T contained meso-2,6 diaminopimelic acid in the cell-wall peptidoglycan, and arabinose and galactose as diagnostic sugars of the whole-cell hydrolysate.
29533177	3	63	from	acid	322:325	arg1	peptidoglycan					344:356	the cell-wall peptidoglycan	330:356	the cell-wall peptidoglycan	330:356	Strain C12CA1T contained meso-2,6 diaminopimelic acid in the cell-wall peptidoglycan, and arabinose and galactose as diagnostic sugars of the whole-cell hydrolysate.
29533177	3	64	contain	contained	288:296	arg2	sugars					401:406	diagnostic sugars	390:406	diagnostic sugars of the whole-cell hydrolysate	390:436	Strain C12CA1T contained meso-2,6 diaminopimelic acid in the cell-wall peptidoglycan, and arabinose and galactose as diagnostic sugars of the whole-cell hydrolysate.
29533177	3	64	contain	contained	288:296	arg2	arabinose					363:371	arabinose	363:371	arabinose	363:371	Strain C12CA1T contained meso-2,6 diaminopimelic acid in the cell-wall peptidoglycan, and arabinose and galactose as diagnostic sugars of the whole-cell hydrolysate.
29533177	3	64	contain	contained	288:296	arg2	acid					322:325	meso-2,6 diaminopimelic acid	298:325	meso-2,6 diaminopimelic acid in the cell-wall peptidoglycan	298:356	Strain C12CA1T contained meso-2,6 diaminopimelic acid in the cell-wall peptidoglycan, and arabinose and galactose as diagnostic sugars of the whole-cell hydrolysate.
29533177	3	64	contain	contained	288:296	arg2	galactose					377:385	galactose	377:385	galactose	377:385	Strain C12CA1T contained meso-2,6 diaminopimelic acid in the cell-wall peptidoglycan, and arabinose and galactose as diagnostic sugars of the whole-cell hydrolysate.
29533177	3	64	contain	contained	288:296	arg1	C12CA1T					280:286	Strain C12CA1T	273:286	Strain C12CA1T	273:286	Strain C12CA1T contained meso-2,6 diaminopimelic acid in the cell-wall peptidoglycan, and arabinose and galactose as diagnostic sugars of the whole-cell hydrolysate.
29533177	7	65	theme	A.	1308:1309	arg1	12673T					1328:1333	A. pretoriensis JCM 12673T	1308:1333	A. pretoriensis JCM 12673T (8.8±0.3-9.2±1.8 %)	1308:1353	The strain exhibited low DNA-DNA relatedness values with A. vancoresmycina DSM 44592T (6.9±0.2-11.6±1.9 %) and A. pretoriensis JCM 12673T (8.8±0.3-9.2±1.8 %).
29533177	7	65	theme	A.	1308:1309	arg1	%					1352:1352	8.8±0.3-9.2±1.8 %	1336:1352	8.8±0.3-9.2±1.8 %	1336:1352	The strain exhibited low DNA-DNA relatedness values with A. vancoresmycina DSM 44592T (6.9±0.2-11.6±1.9 %) and A. pretoriensis JCM 12673T (8.8±0.3-9.2±1.8 %).
29533177	9	66	theme	novel	1490:1494	arg1	species					1496:1502	a novel species	1488:1502	a novel species	1488:1502	Based on the results of polyphasic characterization, strain C12CA1T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsis silviterrae sp.
29533177	9	67	theme	polyphasic	1433:1442	arg1	characterization					1444:1459	polyphasic characterization	1433:1459	polyphasic characterization	1433:1459	Based on the results of polyphasic characterization, strain C12CA1T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsis silviterrae sp.
29533177	5	68	theme	several	693:699	arg1	phospholipids					701:713	several phospholipids	693:713	several phospholipids consisting of diphosphotidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol, hydroxyphosphatidylethanolamine, phosphatidylinositol mannosides and an unidentified glucosamine-containing phospholipid	693:994	It contained MK-9(H4) as the predominant menaquinone, C16 : 0, iso-C15 : 0 and iso-C16 : 0 as the major cellular fatty acids, and several phospholipids consisting of diphosphotidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol, hydroxyphosphatidylethanolamine, phosphatidylinositol mannosides and an unidentified glucosamine-containing phospholipid.
29533177	5	68	theme	several	693:699	arg1	MK-9					576:579	MK-9	576:579	MK-9(H4)	576:583	It contained MK-9(H4) as the predominant menaquinone, C16 : 0, iso-C15 : 0 and iso-C16 : 0 as the major cellular fatty acids, and several phospholipids consisting of diphosphotidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol, hydroxyphosphatidylethanolamine, phosphatidylinositol mannosides and an unidentified glucosamine-containing phospholipid.
29533177	3	69	from	galactose	377:385	arg1	peptidoglycan					344:356	the cell-wall peptidoglycan	330:356	the cell-wall peptidoglycan	330:356	Strain C12CA1T contained meso-2,6 diaminopimelic acid in the cell-wall peptidoglycan, and arabinose and galactose as diagnostic sugars of the whole-cell hydrolysate.
29533177	2	70	theme	taxonomic	207:215	arg1	position					217:224	its taxonomic position	203:224	its taxonomic position	203:224	A novel actinobacterial strain, designated C12CA1T, was isolated from forest soil in the conservation area of Chulabhorn dam, Thailand, and its taxonomic position was determined by using a polyphasic approach.
29533177	6	71	theme	JCM	1175:1177	arg1	12673T					1179:1184	Amycolatopsis pretoriensis JCM 12673T	1148:1184	Amycolatopsis pretoriensis JCM 12673T (98.82 %)	1148:1194	Based on 16S rRNA gene sequence and phylogenetic analyses, strain C12CA1T was closely related to Amycolatopsis vancoresmycina DSM 44592T (98.96 %) and Amycolatopsis pretoriensis JCM 12673T (98.82 %).
29533177	6	71	theme	JCM	1175:1177	arg1	%					1193:1193	98.82 %	1187:1193	98.82 %	1187:1193	Based on 16S rRNA gene sequence and phylogenetic analyses, strain C12CA1T was closely related to Amycolatopsis vancoresmycina DSM 44592T (98.96 %) and Amycolatopsis pretoriensis JCM 12673T (98.82 %).
29533177	9	72	theme	characterization	1444:1459	arg1	results					1422:1428	the results	1418:1428	the results of polyphasic characterization	1418:1459	Based on the results of polyphasic characterization, strain C12CA1T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsis silviterrae sp.
29533177	6	73	theme	rRNA	1010:1013	arg1	sequence					1020:1027	16S rRNA gene sequence	1006:1027	16S rRNA gene sequence	1006:1027	Based on 16S rRNA gene sequence and phylogenetic analyses, strain C12CA1T was closely related to Amycolatopsis vancoresmycina DSM 44592T (98.96 %) and Amycolatopsis pretoriensis JCM 12673T (98.82 %).
31738363	7	0	from	microbiota	1025:1034	arg1	rats					1039:1042	rats	1039:1042	rats	1039:1042	16S rDNA sequencing analysis showed that the structure and composition of gut microbiota in rats were modulated after exposure to TiO2 NPs.
31738363	3	1	theme	gut	501:503	arg1	microbiota					505:514	gut microbiota	501:514	gut microbiota	501:514	We conducted an animal experiment to investigate the effects of oral exposure to TiO2 NPs on gut microbiota and gut-associated metabolism in Sprague-Dawley rats.
31738363	8	2	theme	bacterial	1146:1154	arg1	strains					1156:1162	differentially expressed bacterial strains	1121:1162	differentially expressed bacterial strains	1121:1162	Monitoring data demonstrated that differentially expressed bacterial strains were obtained until exposure for 14 days and 28 days, including increased L. gasseri, Turicibacter, and L. NK4A136_group and decreased Veillonella.
31738363	7	3	from	rats	1039:1042	arg1	composition					1006:1016	composition	1006:1016	composition	1006:1016	16S rDNA sequencing analysis showed that the structure and composition of gut microbiota in rats were modulated after exposure to TiO2 NPs.
31738363	7	3	from	rats	1039:1042	arg1	structure					992:1000	structure	992:1000	structure	992:1000	16S rDNA sequencing analysis showed that the structure and composition of gut microbiota in rats were modulated after exposure to TiO2 NPs.
31738363	6	4	from	changes	824:830	arg1	rats					855:858	rats	855:858	rats	855:858	TiO2 NPs caused significant changes of colon morphology in rats, manifested as pathological inflammatory infiltration and mitochondrial abnormalities.
31738363	11	5	theme	produced	1733:1740	arg1	LPS					1763:1765	LPS	1763:1765	LPS	1763:1765	Metabolic disorders of gut microbiota and subsequently produced lipopolysaccharides (LPS) led to oxidative stress and an inflammatory response in the intestine, which was considered to be a key and primary indirect pathway for toxicity induced by oral exposure to the TiO2 NPs.
31738363	11	5	theme	produced	1733:1740	arg1	lipopolysaccharides					1742:1760	subsequently produced lipopolysaccharides	1720:1760	subsequently produced lipopolysaccharides (LPS)	1720:1766	Metabolic disorders of gut microbiota and subsequently produced lipopolysaccharides (LPS) led to oxidative stress and an inflammatory response in the intestine, which was considered to be a key and primary indirect pathway for toxicity induced by oral exposure to the TiO2 NPs.
31738363	3	6	from	microbiota	505:514	arg1	rats					564:567	Sprague-Dawley rats	549:567	Sprague-Dawley rats	549:567	We conducted an animal experiment to investigate the effects of oral exposure to TiO2 NPs on gut microbiota and gut-associated metabolism in Sprague-Dawley rats.
31738363	3	7	theme	Sprague-Dawley	549:562	arg1	rats					564:567	Sprague-Dawley rats	549:567	Sprague-Dawley rats	549:567	We conducted an animal experiment to investigate the effects of oral exposure to TiO2 NPs on gut microbiota and gut-associated metabolism in Sprague-Dawley rats.
31738363	12	8	theme	gut-associated	2041:2054	arg1	metabolism					2056:2065	gut-associated metabolism	2041:2065	gut-associated metabolism	2041:2065	In conclusion, orally ingested TiO2 NPs could induce disorders of gut microbiota and gut-associated metabolism in vivo.
31738363	6	9	theme	colon	835:839	arg1	morphology					841:850	colon morphology	835:850	colon morphology	835:850	TiO2 NPs caused significant changes of colon morphology in rats, manifested as pathological inflammatory infiltration and mitochondrial abnormalities.
31738363	5	10	dep	gut	720:722	arg1	microbiota					724:733	microbiota	724:733	microbiota	724:733	Changes in the gut microbiota and feces metabolomics were analyzed through bioinformatics.
31738363	5	10	dep	gut	720:722	arg1	metabolomics					745:756	metabolomics	745:756	metabolomics	745:756	Changes in the gut microbiota and feces metabolomics were analyzed through bioinformatics.
31738363	4	11	theme	exposure	648:655	arg1	kg-1					680:683	0, 2, 10, 50 mg kg-1	664:683	0, 2, 10, 50 mg kg-1	664:683	Rats were administered with TiO2 NPs (29 ± 9 nm) orally at population-related exposure doses (0, 2, 10, 50 mg kg-1) daily for 30 days.
31738363	4	11	theme	exposure	648:655	arg1	doses					657:661	population-related exposure doses	629:661	population-related exposure doses (0, 2, 10, 50 mg kg-1)	629:684	Rats were administered with TiO2 NPs (29 ± 9 nm) orally at population-related exposure doses (0, 2, 10, 50 mg kg-1) daily for 30 days.
31738363	9	12	theme	exposed	1451:1457	arg1	rats					1459:1462	exposed rats	1451:1462	exposed rats	1451:1462	Fecal metabolomics analysis showed that 25 metabolites and the aminoacyl-tRNA biosynthesis metabolic pathway have changed significantly in exposed rats.
31738363	12	13	theme	microbiota	2026:2035	arg1	disorders					2009:2017	disorders	2009:2017	disorders of gut microbiota and gut-associated metabolism	2009:2065	In conclusion, orally ingested TiO2 NPs could induce disorders of gut microbiota and gut-associated metabolism in vivo.
31738363	14	14	theme	most	2368:2371	arg1	nanomaterials					2373:2385	most nanomaterials	2368:2385	most nanomaterials	2368:2385	This may be a common mechanism of toxicity caused by oral administration of most nanomaterials, as they usually have potential antimicrobial activity.
31738363	2	15	theme	potential	349:357	arg1	risks					366:370	potential health risks	349:370	potential health risks	349:370	Ingestion of TiO2 NPs via dietary and environmental exposure may pose potential health risks by interacting with gut microbiota.
31738363	12	16	theme	ingested	1978:1985	arg1	NPs					1992:1994	orally ingested TiO2 NPs	1971:1994	orally ingested TiO2 NPs	1971:1994	In conclusion, orally ingested TiO2 NPs could induce disorders of gut microbiota and gut-associated metabolism in vivo.
31738363	8	17	theme	Turicibacter	1250:1261	arg1	NK4A136_group					1271:1283	L. gasseri, Turicibacter, and L. NK4A136_group	1238:1283	L. gasseri, Turicibacter, and L. NK4A136_group	1238:1283	Monitoring data demonstrated that differentially expressed bacterial strains were obtained until exposure for 14 days and 28 days, including increased L. gasseri, Turicibacter, and L. NK4A136_group and decreased Veillonella.
31738363	6	18	theme	inflammatory	888:899	arg1	infiltration					901:912	pathological inflammatory infiltration	875:912	pathological inflammatory infiltration	875:912	TiO2 NPs caused significant changes of colon morphology in rats, manifested as pathological inflammatory infiltration and mitochondrial abnormalities.
31738363	7	19	theme	gut	1021:1023	arg1	microbiota					1025:1034	gut microbiota	1021:1034	gut microbiota in rats	1021:1042	16S rDNA sequencing analysis showed that the structure and composition of gut microbiota in rats were modulated after exposure to TiO2 NPs.
31738363	13	20	theme	response	2134:2141	arg1	pathway					2089:2095	The indirect pathway	2076:2095	The indirect pathway of oxidative stress and inflammatory response, probably due to dysbiosis of gut microbiota primarily,	2076:2197	The indirect pathway of oxidative stress and inflammatory response, probably due to dysbiosis of gut microbiota primarily, played an important role in the mechanisms of toxicity induced by oral exposure to TiO2 NPs.
31738363	11	21	from	response	1812:1819	arg1	intestine					1828:1836	the intestine	1824:1836	the intestine	1824:1836	Metabolic disorders of gut microbiota and subsequently produced lipopolysaccharides (LPS) led to oxidative stress and an inflammatory response in the intestine, which was considered to be a key and primary indirect pathway for toxicity induced by oral exposure to the TiO2 NPs.
31738363	14	22	theme	oral	2345:2348	arg1	administration					2350:2363	oral administration	2345:2363	oral administration of most nanomaterials	2345:2385	This may be a common mechanism of toxicity caused by oral administration of most nanomaterials, as they usually have potential antimicrobial activity.
31738363	7	23	theme	sequencing	956:965	arg1	analysis					967:974	16S rDNA sequencing analysis	947:974	16S rDNA sequencing analysis	947:974	16S rDNA sequencing analysis showed that the structure and composition of gut microbiota in rats were modulated after exposure to TiO2 NPs.
31738363	4	24	dep	kg-1	680:683	arg1	mg					677:678	mg	677:678	mg	677:678	Rats were administered with TiO2 NPs (29 ± 9 nm) orally at population-related exposure doses (0, 2, 10, 50 mg kg-1) daily for 30 days.
31738363	13	25	theme	oxidative	2100:2108	arg1	stress					2110:2115	oxidative stress	2100:2115	oxidative stress	2100:2115	The indirect pathway of oxidative stress and inflammatory response, probably due to dysbiosis of gut microbiota primarily, played an important role in the mechanisms of toxicity induced by oral exposure to TiO2 NPs.
31738363	7	26	theme	16S	947:949	arg1	sequencing					956:965	16S rDNA sequencing	947:965	16S rDNA sequencing analysis	947:974	16S rDNA sequencing analysis showed that the structure and composition of gut microbiota in rats were modulated after exposure to TiO2 NPs.
31738363	1	27	theme	titanium	146:153	arg1	dioxide					155:161	titanium dioxide	146:161	titanium dioxide nanoparticles (TiO2 NPs)	146:186	The antibacterial activity of titanium dioxide nanoparticles (TiO2 NPs) has been extensively documented and applied to food packaging or environmental protection.
31738363	12	28	theme	gut	2022:2024	arg1	microbiota					2026:2035	gut microbiota	2022:2035	gut microbiota	2022:2035	In conclusion, orally ingested TiO2 NPs could induce disorders of gut microbiota and gut-associated metabolism in vivo.
31738363	11	29	from	stress	1785:1790	arg1	intestine					1828:1836	the intestine	1824:1836	the intestine	1824:1836	Metabolic disorders of gut microbiota and subsequently produced lipopolysaccharides (LPS) led to oxidative stress and an inflammatory response in the intestine, which was considered to be a key and primary indirect pathway for toxicity induced by oral exposure to the TiO2 NPs.
31738363	3	30	from	metabolism	535:544	arg1	rats					564:567	Sprague-Dawley rats	549:567	Sprague-Dawley rats	549:567	We conducted an animal experiment to investigate the effects of oral exposure to TiO2 NPs on gut microbiota and gut-associated metabolism in Sprague-Dawley rats.
31738363	1	31	theme	nanoparticles	163:175	arg1	activity					134:141	The antibacterial activity	116:141	The antibacterial activity of titanium dioxide nanoparticles (TiO2 NPs)	116:186	The antibacterial activity of titanium dioxide nanoparticles (TiO2 NPs) has been extensively documented and applied to food packaging or environmental protection.
31738363	14	32	theme	common	2306:2311	arg1	mechanism					2313:2321	a common mechanism	2304:2321	a common mechanism of toxicity caused by oral administration of most nanomaterials	2304:2385	This may be a common mechanism of toxicity caused by oral administration of most nanomaterials, as they usually have potential antimicrobial activity.
31738363	14	32	theme	common	2306:2311	arg1	This					2292:2295	This	2292:2295	This	2292:2295	This may be a common mechanism of toxicity caused by oral administration of most nanomaterials, as they usually have potential antimicrobial activity.
31738363	0	33	theme	gut-associated	81:94	arg1	metabolism					96:105	gut-associated metabolism	81:105	gut-associated metabolism	81:105	Effects of oral exposure to titanium dioxide nanoparticles on gut microbiota and gut-associated metabolism in vivo.
31738363	11	34	theme	TiO2	1946:1949	arg1	NPs					1951:1953	the TiO2 NPs	1942:1953	the TiO2 NPs	1942:1953	Metabolic disorders of gut microbiota and subsequently produced lipopolysaccharides (LPS) led to oxidative stress and an inflammatory response in the intestine, which was considered to be a key and primary indirect pathway for toxicity induced by oral exposure to the TiO2 NPs.
31738363	8	35	dep	L.	1238:1239	arg1	gasseri					1241:1247	L. gasseri	1238:1247	L. gasseri	1238:1247	Monitoring data demonstrated that differentially expressed bacterial strains were obtained until exposure for 14 days and 28 days, including increased L. gasseri, Turicibacter, and L. NK4A136_group and decreased Veillonella.
31738363	10	36	theme	increased	1469:1477	arg1	metabolites					1479:1489	The increased metabolites	1465:1489	The increased metabolites	1465:1489	The increased metabolites were represented by N-acetylhistamine, caprolactam, and glycerophosphocholine, and the decreased metabolites were represented by 4-methyl-5-thiazoleethanol, l-histidine, and l-ornithine.
31738363	7	37	dep	structure	992:1000	arg1	the					988:990	the	988:990	the	988:990	16S rDNA sequencing analysis showed that the structure and composition of gut microbiota in rats were modulated after exposure to TiO2 NPs.
31738363	3	38	theme	animal	424:429	arg1	experiment					431:440	an animal experiment	421:440	an animal experiment	421:440	We conducted an animal experiment to investigate the effects of oral exposure to TiO2 NPs on gut microbiota and gut-associated metabolism in Sprague-Dawley rats.
31738363	9	39	theme	Fecal	1312:1316	arg1	analysis					1331:1338	Fecal metabolomics analysis	1312:1338	Fecal metabolomics analysis	1312:1338	Fecal metabolomics analysis showed that 25 metabolites and the aminoacyl-tRNA biosynthesis metabolic pathway have changed significantly in exposed rats.
31738363	0	40	theme	oral	11:14	arg1	exposure					16:23	oral exposure	11:23	oral exposure to titanium dioxide	11:43	Effects of oral exposure to titanium dioxide nanoparticles on gut microbiota and gut-associated metabolism in vivo.
31738363	13	41	theme	oral	2265:2268	arg1	exposure					2270:2277	oral exposure	2265:2277	oral exposure to TiO2 NPs	2265:2289	The indirect pathway of oxidative stress and inflammatory response, probably due to dysbiosis of gut microbiota primarily, played an important role in the mechanisms of toxicity induced by oral exposure to TiO2 NPs.
31738363	6	42	theme	significant	812:822	arg1	changes					824:830	significant changes	812:830	significant changes of colon morphology in rats, manifested as pathological inflammatory infiltration and mitochondrial abnormalities	812:944	TiO2 NPs caused significant changes of colon morphology in rats, manifested as pathological inflammatory infiltration and mitochondrial abnormalities.
31738363	3	43	from	effects	461:467	arg1	microbiota					505:514	gut microbiota	501:514	gut microbiota	501:514	We conducted an animal experiment to investigate the effects of oral exposure to TiO2 NPs on gut microbiota and gut-associated metabolism in Sprague-Dawley rats.
31738363	3	43	from	effects	461:467	arg1	metabolism					535:544	gut-associated metabolism	520:544	gut-associated metabolism	520:544	We conducted an animal experiment to investigate the effects of oral exposure to TiO2 NPs on gut microbiota and gut-associated metabolism in Sprague-Dawley rats.
31738363	3	44	theme	TiO2	489:492	arg1	NPs					494:496	TiO2 NPs	489:496	TiO2 NPs	489:496	We conducted an animal experiment to investigate the effects of oral exposure to TiO2 NPs on gut microbiota and gut-associated metabolism in Sprague-Dawley rats.
31738363	3	45	theme	exposure	477:484	arg1	effects					461:467	the effects	457:467	the effects of oral exposure to TiO2 NPs on gut microbiota and gut-associated metabolism in Sprague-Dawley rats	457:567	We conducted an animal experiment to investigate the effects of oral exposure to TiO2 NPs on gut microbiota and gut-associated metabolism in Sprague-Dawley rats.
31738363	13	46	theme	TiO2	2282:2285	arg1	NPs					2287:2289	TiO2 NPs	2282:2289	TiO2 NPs	2282:2289	The indirect pathway of oxidative stress and inflammatory response, probably due to dysbiosis of gut microbiota primarily, played an important role in the mechanisms of toxicity induced by oral exposure to TiO2 NPs.
31738363	7	47	from	composition	1006:1016	arg1	rats					1039:1042	rats	1039:1042	rats	1039:1042	16S rDNA sequencing analysis showed that the structure and composition of gut microbiota in rats were modulated after exposure to TiO2 NPs.
31738363	10	48	theme	decreased	1578:1586	arg1	metabolites					1588:1598	the decreased metabolites	1574:1598	the decreased metabolites	1574:1598	The increased metabolites were represented by N-acetylhistamine, caprolactam, and glycerophosphocholine, and the decreased metabolites were represented by 4-methyl-5-thiazoleethanol, l-histidine, and l-ornithine.
31738363	11	49	theme	oxidative	1775:1783	arg1	response					1812:1819	an inflammatory response	1796:1819	an inflammatory response	1796:1819	Metabolic disorders of gut microbiota and subsequently produced lipopolysaccharides (LPS) led to oxidative stress and an inflammatory response in the intestine, which was considered to be a key and primary indirect pathway for toxicity induced by oral exposure to the TiO2 NPs.
31738363	11	49	theme	oxidative	1775:1783	arg1	stress					1785:1790	oxidative stress	1775:1790	oxidative stress	1775:1790	Metabolic disorders of gut microbiota and subsequently produced lipopolysaccharides (LPS) led to oxidative stress and an inflammatory response in the intestine, which was considered to be a key and primary indirect pathway for toxicity induced by oral exposure to the TiO2 NPs.
31738363	11	49	theme	oxidative	1775:1783	arg1	pathway					1893:1899	a key and primary indirect pathway	1866:1899	a key and primary indirect pathway for toxicity induced by oral exposure to the TiO2 NPs	1866:1953	Metabolic disorders of gut microbiota and subsequently produced lipopolysaccharides (LPS) led to oxidative stress and an inflammatory response in the intestine, which was considered to be a key and primary indirect pathway for toxicity induced by oral exposure to the TiO2 NPs.
31738363	11	50	theme	Metabolic	1678:1686	arg1	disorders					1688:1696	Metabolic disorders	1678:1696	Metabolic disorders of gut microbiota	1678:1714	Metabolic disorders of gut microbiota and subsequently produced lipopolysaccharides (LPS) led to oxidative stress and an inflammatory response in the intestine, which was considered to be a key and primary indirect pathway for toxicity induced by oral exposure to the TiO2 NPs.
31738363	11	51	theme	oral	1925:1928	arg1	exposure					1930:1937	oral exposure	1925:1937	oral exposure to the TiO2 NPs	1925:1953	Metabolic disorders of gut microbiota and subsequently produced lipopolysaccharides (LPS) led to oxidative stress and an inflammatory response in the intestine, which was considered to be a key and primary indirect pathway for toxicity induced by oral exposure to the TiO2 NPs.
31738363	1	52	theme	TiO2	178:181	arg1	NPs					183:185	TiO2 NPs	178:185	TiO2 NPs	178:185	The antibacterial activity of titanium dioxide nanoparticles (TiO2 NPs) has been extensively documented and applied to food packaging or environmental protection.
31738363	1	52	theme	TiO2	178:181	arg1	nanoparticles					163:175	titanium dioxide nanoparticles	146:175	titanium dioxide nanoparticles (TiO2 NPs)	146:186	The antibacterial activity of titanium dioxide nanoparticles (TiO2 NPs) has been extensively documented and applied to food packaging or environmental protection.
31738363	9	53	theme	biosynthesis	1390:1401	arg1	pathway					1413:1419	the aminoacyl-tRNA biosynthesis metabolic pathway	1371:1419	the aminoacyl-tRNA biosynthesis metabolic pathway	1371:1419	Fecal metabolomics analysis showed that 25 metabolites and the aminoacyl-tRNA biosynthesis metabolic pathway have changed significantly in exposed rats.
31738363	12	54	theme	metabolism	2056:2065	arg1	disorders					2009:2017	disorders	2009:2017	disorders of gut microbiota and gut-associated metabolism	2009:2065	In conclusion, orally ingested TiO2 NPs could induce disorders of gut microbiota and gut-associated metabolism in vivo.
31738363	4	55	theme	±	611:611	arg1	nm					615:616	29 ± 9 nm	608:616	29 ± 9 nm	608:616	Rats were administered with TiO2 NPs (29 ± 9 nm) orally at population-related exposure doses (0, 2, 10, 50 mg kg-1) daily for 30 days.
31738363	4	55	theme	±	611:611	arg1	NPs					603:605	TiO2 NPs	598:605	TiO2 NPs (29 ± 9 nm)	598:617	Rats were administered with TiO2 NPs (29 ± 9 nm) orally at population-related exposure doses (0, 2, 10, 50 mg kg-1) daily for 30 days.
31738363	11	56	theme	microbiota	1705:1714	arg1	disorders					1688:1696	Metabolic disorders	1678:1696	Metabolic disorders of gut microbiota	1678:1714	Metabolic disorders of gut microbiota and subsequently produced lipopolysaccharides (LPS) led to oxidative stress and an inflammatory response in the intestine, which was considered to be a key and primary indirect pathway for toxicity induced by oral exposure to the TiO2 NPs.
31738363	11	56	theme	microbiota	1705:1714	arg1	LPS					1763:1765	LPS	1763:1765	LPS	1763:1765	Metabolic disorders of gut microbiota and subsequently produced lipopolysaccharides (LPS) led to oxidative stress and an inflammatory response in the intestine, which was considered to be a key and primary indirect pathway for toxicity induced by oral exposure to the TiO2 NPs.
31738363	11	56	theme	microbiota	1705:1714	arg1	lipopolysaccharides					1742:1760	subsequently produced lipopolysaccharides	1720:1760	subsequently produced lipopolysaccharides (LPS)	1720:1766	Metabolic disorders of gut microbiota and subsequently produced lipopolysaccharides (LPS) led to oxidative stress and an inflammatory response in the intestine, which was considered to be a key and primary indirect pathway for toxicity induced by oral exposure to the TiO2 NPs.
31738363	14	57	theme	potential	2409:2417	arg1	activity					2433:2440	potential antimicrobial activity	2409:2440	potential antimicrobial activity	2409:2440	This may be a common mechanism of toxicity caused by oral administration of most nanomaterials, as they usually have potential antimicrobial activity.
31738363	2	58	theme	health	359:364	arg1	risks					366:370	potential health risks	349:370	potential health risks	349:370	Ingestion of TiO2 NPs via dietary and environmental exposure may pose potential health risks by interacting with gut microbiota.
31738363	13	59	theme	microbiota	2177:2186	arg1	dysbiosis					2160:2168	dysbiosis	2160:2168	dysbiosis of gut microbiota	2160:2186	The indirect pathway of oxidative stress and inflammatory response, probably due to dysbiosis of gut microbiota primarily, played an important role in the mechanisms of toxicity induced by oral exposure to TiO2 NPs.
31738363	7	60	theme	TiO2	1077:1080	arg1	NPs					1082:1084	TiO2 NPs	1077:1084	TiO2 NPs	1077:1084	16S rDNA sequencing analysis showed that the structure and composition of gut microbiota in rats were modulated after exposure to TiO2 NPs.
31738363	2	61	theme	TiO2	292:295	arg1	NPs					297:299	TiO2 NPs	292:299	TiO2 NPs	292:299	Ingestion of TiO2 NPs via dietary and environmental exposure may pose potential health risks by interacting with gut microbiota.
31738363	1	62	theme	environmental	253:265	arg1	protection					267:276	environmental protection	253:276	environmental protection	253:276	The antibacterial activity of titanium dioxide nanoparticles (TiO2 NPs) has been extensively documented and applied to food packaging or environmental protection.
31738363	8	63	theme	expressed	1136:1144	arg1	strains					1156:1162	differentially expressed bacterial strains	1121:1162	differentially expressed bacterial strains	1121:1162	Monitoring data demonstrated that differentially expressed bacterial strains were obtained until exposure for 14 days and 28 days, including increased L. gasseri, Turicibacter, and L. NK4A136_group and decreased Veillonella.
31738363	6	64	theme	morphology	841:850	arg1	changes					824:830	significant changes	812:830	significant changes of colon morphology in rats, manifested as pathological inflammatory infiltration and mitochondrial abnormalities	812:944	TiO2 NPs caused significant changes of colon morphology in rats, manifested as pathological inflammatory infiltration and mitochondrial abnormalities.
31738363	14	65	contain	have	2404:2407	arg1	they					2391:2394	they	2391:2394	they	2391:2394	This may be a common mechanism of toxicity caused by oral administration of most nanomaterials, as they usually have potential antimicrobial activity.
31738363	14	65	contain	have	2404:2407	arg2	activity					2433:2440	potential antimicrobial activity	2409:2440	potential antimicrobial activity	2409:2440	This may be a common mechanism of toxicity caused by oral administration of most nanomaterials, as they usually have potential antimicrobial activity.
31738363	4	66	theme	TiO2	598:601	arg1	nm					615:616	29 ± 9 nm	608:616	29 ± 9 nm	608:616	Rats were administered with TiO2 NPs (29 ± 9 nm) orally at population-related exposure doses (0, 2, 10, 50 mg kg-1) daily for 30 days.
31738363	4	66	theme	TiO2	598:601	arg1	NPs					603:605	TiO2 NPs	598:605	TiO2 NPs (29 ± 9 nm)	598:617	Rats were administered with TiO2 NPs (29 ± 9 nm) orally at population-related exposure doses (0, 2, 10, 50 mg kg-1) daily for 30 days.
31738363	3	67	theme	gut-associated	520:533	arg1	metabolism					535:544	gut-associated metabolism	520:544	gut-associated metabolism	520:544	We conducted an animal experiment to investigate the effects of oral exposure to TiO2 NPs on gut microbiota and gut-associated metabolism in Sprague-Dawley rats.
31738363	4	68	theme	population-related	629:646	arg1	kg-1					680:683	0, 2, 10, 50 mg kg-1	664:683	0, 2, 10, 50 mg kg-1	664:683	Rats were administered with TiO2 NPs (29 ± 9 nm) orally at population-related exposure doses (0, 2, 10, 50 mg kg-1) daily for 30 days.
31738363	4	68	theme	population-related	629:646	arg1	doses					657:661	population-related exposure doses	629:661	population-related exposure doses (0, 2, 10, 50 mg kg-1)	629:684	Rats were administered with TiO2 NPs (29 ± 9 nm) orally at population-related exposure doses (0, 2, 10, 50 mg kg-1) daily for 30 days.
31738363	14	69	theme	nanomaterials	2373:2385	arg1	administration					2350:2363	oral administration	2345:2363	oral administration of most nanomaterials	2345:2385	This may be a common mechanism of toxicity caused by oral administration of most nanomaterials, as they usually have potential antimicrobial activity.
31738363	6	70	theme	mitochondrial	918:930	arg1	abnormalities					932:944	mitochondrial abnormalities	918:944	mitochondrial abnormalities	918:944	TiO2 NPs caused significant changes of colon morphology in rats, manifested as pathological inflammatory infiltration and mitochondrial abnormalities.
31738363	8	71	theme	L.	1238:1239	arg1	NK4A136_group					1271:1283	L. gasseri, Turicibacter, and L. NK4A136_group	1238:1283	L. gasseri, Turicibacter, and L. NK4A136_group	1238:1283	Monitoring data demonstrated that differentially expressed bacterial strains were obtained until exposure for 14 days and 28 days, including increased L. gasseri, Turicibacter, and L. NK4A136_group and decreased Veillonella.
31738363	7	72	theme	microbiota	1025:1034	arg1	composition					1006:1016	composition	1006:1016	composition	1006:1016	16S rDNA sequencing analysis showed that the structure and composition of gut microbiota in rats were modulated after exposure to TiO2 NPs.
31738363	7	72	theme	microbiota	1025:1034	arg1	structure					992:1000	structure	992:1000	structure	992:1000	16S rDNA sequencing analysis showed that the structure and composition of gut microbiota in rats were modulated after exposure to TiO2 NPs.
31738363	7	73	from	structure	992:1000	arg1	rats					1039:1042	rats	1039:1042	rats	1039:1042	16S rDNA sequencing analysis showed that the structure and composition of gut microbiota in rats were modulated after exposure to TiO2 NPs.
31738363	11	74	theme	primary	1876:1882	arg1	response					1812:1819	an inflammatory response	1796:1819	an inflammatory response	1796:1819	Metabolic disorders of gut microbiota and subsequently produced lipopolysaccharides (LPS) led to oxidative stress and an inflammatory response in the intestine, which was considered to be a key and primary indirect pathway for toxicity induced by oral exposure to the TiO2 NPs.
31738363	11	74	theme	primary	1876:1882	arg1	stress					1785:1790	oxidative stress	1775:1790	oxidative stress	1775:1790	Metabolic disorders of gut microbiota and subsequently produced lipopolysaccharides (LPS) led to oxidative stress and an inflammatory response in the intestine, which was considered to be a key and primary indirect pathway for toxicity induced by oral exposure to the TiO2 NPs.
31738363	11	74	theme	primary	1876:1882	arg1	pathway					1893:1899	a key and primary indirect pathway	1866:1899	a key and primary indirect pathway for toxicity induced by oral exposure to the TiO2 NPs	1866:1953	Metabolic disorders of gut microbiota and subsequently produced lipopolysaccharides (LPS) led to oxidative stress and an inflammatory response in the intestine, which was considered to be a key and primary indirect pathway for toxicity induced by oral exposure to the TiO2 NPs.
31738363	13	75	theme	gut	2173:2175	arg1	microbiota					2177:2186	gut microbiota	2173:2186	gut microbiota	2173:2186	The indirect pathway of oxidative stress and inflammatory response, probably due to dysbiosis of gut microbiota primarily, played an important role in the mechanisms of toxicity induced by oral exposure to TiO2 NPs.
31738363	12	76	theme	TiO2	1987:1990	arg1	NPs					1992:1994	orally ingested TiO2 NPs	1971:1994	orally ingested TiO2 NPs	1971:1994	In conclusion, orally ingested TiO2 NPs could induce disorders of gut microbiota and gut-associated metabolism in vivo.
31738363	6	77	theme	pathological	875:886	arg1	infiltration					901:912	pathological inflammatory infiltration	875:912	pathological inflammatory infiltration	875:912	TiO2 NPs caused significant changes of colon morphology in rats, manifested as pathological inflammatory infiltration and mitochondrial abnormalities.
31738363	11	78	theme	key	1868:1870	arg1	response					1812:1819	an inflammatory response	1796:1819	an inflammatory response	1796:1819	Metabolic disorders of gut microbiota and subsequently produced lipopolysaccharides (LPS) led to oxidative stress and an inflammatory response in the intestine, which was considered to be a key and primary indirect pathway for toxicity induced by oral exposure to the TiO2 NPs.
31738363	11	78	theme	key	1868:1870	arg1	stress					1785:1790	oxidative stress	1775:1790	oxidative stress	1775:1790	Metabolic disorders of gut microbiota and subsequently produced lipopolysaccharides (LPS) led to oxidative stress and an inflammatory response in the intestine, which was considered to be a key and primary indirect pathway for toxicity induced by oral exposure to the TiO2 NPs.
31738363	11	78	theme	key	1868:1870	arg1	pathway					1893:1899	a key and primary indirect pathway	1866:1899	a key and primary indirect pathway for toxicity induced by oral exposure to the TiO2 NPs	1866:1953	Metabolic disorders of gut microbiota and subsequently produced lipopolysaccharides (LPS) led to oxidative stress and an inflammatory response in the intestine, which was considered to be a key and primary indirect pathway for toxicity induced by oral exposure to the TiO2 NPs.
31738363	5	79	from	Changes	705:711	arg1	gut					720:722	the gut microbiota and feces metabolomics	716:756	gut	720:722	Changes in the gut microbiota and feces metabolomics were analyzed through bioinformatics.
31738363	5	79	from	Changes	705:711	arg1	feces					739:743	feces	739:743	feces	739:743	Changes in the gut microbiota and feces metabolomics were analyzed through bioinformatics.
31738363	1	80	theme	antibacterial	120:132	arg1	activity					134:141	The antibacterial activity	116:141	The antibacterial activity of titanium dioxide nanoparticles (TiO2 NPs)	116:186	The antibacterial activity of titanium dioxide nanoparticles (TiO2 NPs) has been extensively documented and applied to food packaging or environmental protection.
31738363	8	81	theme	L.	1268:1269	arg1	NK4A136_group					1271:1283	L. gasseri, Turicibacter, and L. NK4A136_group	1238:1283	L. gasseri, Turicibacter, and L. NK4A136_group	1238:1283	Monitoring data demonstrated that differentially expressed bacterial strains were obtained until exposure for 14 days and 28 days, including increased L. gasseri, Turicibacter, and L. NK4A136_group and decreased Veillonella.
31738363	11	82	theme	indirect	1884:1891	arg1	response					1812:1819	an inflammatory response	1796:1819	an inflammatory response	1796:1819	Metabolic disorders of gut microbiota and subsequently produced lipopolysaccharides (LPS) led to oxidative stress and an inflammatory response in the intestine, which was considered to be a key and primary indirect pathway for toxicity induced by oral exposure to the TiO2 NPs.
31738363	11	82	theme	indirect	1884:1891	arg1	stress					1785:1790	oxidative stress	1775:1790	oxidative stress	1775:1790	Metabolic disorders of gut microbiota and subsequently produced lipopolysaccharides (LPS) led to oxidative stress and an inflammatory response in the intestine, which was considered to be a key and primary indirect pathway for toxicity induced by oral exposure to the TiO2 NPs.
31738363	11	82	theme	indirect	1884:1891	arg1	pathway					1893:1899	a key and primary indirect pathway	1866:1899	a key and primary indirect pathway for toxicity induced by oral exposure to the TiO2 NPs	1866:1953	Metabolic disorders of gut microbiota and subsequently produced lipopolysaccharides (LPS) led to oxidative stress and an inflammatory response in the intestine, which was considered to be a key and primary indirect pathway for toxicity induced by oral exposure to the TiO2 NPs.
31738363	0	83	theme	gut	62:64	arg1	microbiota					66:75	gut microbiota	62:75	gut microbiota	62:75	Effects of oral exposure to titanium dioxide nanoparticles on gut microbiota and gut-associated metabolism in vivo.
31738363	13	84	theme	due	2153:2155	arg1	stress					2110:2115	oxidative stress	2100:2115	oxidative stress	2100:2115	The indirect pathway of oxidative stress and inflammatory response, probably due to dysbiosis of gut microbiota primarily, played an important role in the mechanisms of toxicity induced by oral exposure to TiO2 NPs.
31738363	14	85	theme	toxicity	2326:2333	arg1	mechanism					2313:2321	a common mechanism	2304:2321	a common mechanism of toxicity caused by oral administration of most nanomaterials	2304:2385	This may be a common mechanism of toxicity caused by oral administration of most nanomaterials, as they usually have potential antimicrobial activity.
31738363	14	85	theme	toxicity	2326:2333	arg1	This					2292:2295	This	2292:2295	This	2292:2295	This may be a common mechanism of toxicity caused by oral administration of most nanomaterials, as they usually have potential antimicrobial activity.
31738363	11	86	theme	inflammatory	1799:1810	arg1	response					1812:1819	an inflammatory response	1796:1819	an inflammatory response	1796:1819	Metabolic disorders of gut microbiota and subsequently produced lipopolysaccharides (LPS) led to oxidative stress and an inflammatory response in the intestine, which was considered to be a key and primary indirect pathway for toxicity induced by oral exposure to the TiO2 NPs.
31738363	11	86	theme	inflammatory	1799:1810	arg1	stress					1785:1790	oxidative stress	1775:1790	oxidative stress	1775:1790	Metabolic disorders of gut microbiota and subsequently produced lipopolysaccharides (LPS) led to oxidative stress and an inflammatory response in the intestine, which was considered to be a key and primary indirect pathway for toxicity induced by oral exposure to the TiO2 NPs.
31738363	11	86	theme	inflammatory	1799:1810	arg1	pathway					1893:1899	a key and primary indirect pathway	1866:1899	a key and primary indirect pathway for toxicity induced by oral exposure to the TiO2 NPs	1866:1953	Metabolic disorders of gut microbiota and subsequently produced lipopolysaccharides (LPS) led to oxidative stress and an inflammatory response in the intestine, which was considered to be a key and primary indirect pathway for toxicity induced by oral exposure to the TiO2 NPs.
31738363	13	87	theme	stress	2110:2115	arg1	pathway					2089:2095	The indirect pathway	2076:2095	The indirect pathway of oxidative stress and inflammatory response, probably due to dysbiosis of gut microbiota primarily,	2076:2197	The indirect pathway of oxidative stress and inflammatory response, probably due to dysbiosis of gut microbiota primarily, played an important role in the mechanisms of toxicity induced by oral exposure to TiO2 NPs.
31738363	7	88	theme	rDNA	951:954	arg1	sequencing					956:965	16S rDNA sequencing	947:965	16S rDNA sequencing analysis	947:974	16S rDNA sequencing analysis showed that the structure and composition of gut microbiota in rats were modulated after exposure to TiO2 NPs.
31738363	1	89	theme	dioxide	155:161	arg1	NPs					183:185	TiO2 NPs	178:185	TiO2 NPs	178:185	The antibacterial activity of titanium dioxide nanoparticles (TiO2 NPs) has been extensively documented and applied to food packaging or environmental protection.
31738363	1	89	theme	dioxide	155:161	arg1	nanoparticles					163:175	titanium dioxide nanoparticles	146:175	titanium dioxide nanoparticles (TiO2 NPs)	146:186	The antibacterial activity of titanium dioxide nanoparticles (TiO2 NPs) has been extensively documented and applied to food packaging or environmental protection.
31738363	13	90	theme	inflammatory	2121:2132	arg1	response					2134:2141	inflammatory response	2121:2141	inflammatory response	2121:2141	The indirect pathway of oxidative stress and inflammatory response, probably due to dysbiosis of gut microbiota primarily, played an important role in the mechanisms of toxicity induced by oral exposure to TiO2 NPs.
31738363	2	91	theme	NPs	297:299	arg1	Ingestion					279:287	Ingestion	279:287	Ingestion of TiO2 NPs via dietary and environmental exposure	279:338	Ingestion of TiO2 NPs via dietary and environmental exposure may pose potential health risks by interacting with gut microbiota.
31738363	13	92	theme	indirect	2080:2087	arg1	pathway					2089:2095	The indirect pathway	2076:2095	The indirect pathway of oxidative stress and inflammatory response, probably due to dysbiosis of gut microbiota primarily,	2076:2197	The indirect pathway of oxidative stress and inflammatory response, probably due to dysbiosis of gut microbiota primarily, played an important role in the mechanisms of toxicity induced by oral exposure to TiO2 NPs.
31738363	13	93	theme	important	2209:2217	arg1	role					2219:2222	an important role	2206:2222	an important role	2206:2222	The indirect pathway of oxidative stress and inflammatory response, probably due to dysbiosis of gut microbiota primarily, played an important role in the mechanisms of toxicity induced by oral exposure to TiO2 NPs.
31738363	2	94	theme	gut	392:394	arg1	microbiota					396:405	gut microbiota	392:405	gut microbiota	392:405	Ingestion of TiO2 NPs via dietary and environmental exposure may pose potential health risks by interacting with gut microbiota.
31738363	4	95	dep	mg	677:678	arg1	50					674:675	50	674:675	50	674:675	Rats were administered with TiO2 NPs (29 ± 9 nm) orally at population-related exposure doses (0, 2, 10, 50 mg kg-1) daily for 30 days.
31738363	0	96	theme	exposure	16:23	arg1	Effects					0:6	Effects	0:6	Effects of oral exposure to titanium dioxide	0:43	Effects of oral exposure to titanium dioxide nanoparticles on gut microbiota and gut-associated metabolism in vivo.
31738363	9	97	theme	metabolomics	1318:1329	arg1	analysis					1331:1338	Fecal metabolomics analysis	1312:1338	Fecal metabolomics analysis	1312:1338	Fecal metabolomics analysis showed that 25 metabolites and the aminoacyl-tRNA biosynthesis metabolic pathway have changed significantly in exposed rats.
31738363	3	98	theme	oral	472:475	arg1	exposure					477:484	oral exposure	472:484	oral exposure to TiO2 NPs	472:496	We conducted an animal experiment to investigate the effects of oral exposure to TiO2 NPs on gut microbiota and gut-associated metabolism in Sprague-Dawley rats.
31738363	0	99	theme	titanium	28:35	arg1	dioxide					37:43	titanium dioxide	28:43	titanium dioxide	28:43	Effects of oral exposure to titanium dioxide nanoparticles on gut microbiota and gut-associated metabolism in vivo.
31738363	6	100	theme	TiO2	796:799	arg1	NPs					801:803	TiO2 NPs	796:803	TiO2 NPs	796:803	TiO2 NPs caused significant changes of colon morphology in rats, manifested as pathological inflammatory infiltration and mitochondrial abnormalities.
31738363	13	101	theme	toxicity	2245:2252	arg1	mechanisms					2231:2240	the mechanisms	2227:2240	the mechanisms of toxicity induced by oral exposure to TiO2 NPs	2227:2289	The indirect pathway of oxidative stress and inflammatory response, probably due to dysbiosis of gut microbiota primarily, played an important role in the mechanisms of toxicity induced by oral exposure to TiO2 NPs.
31738363	8	102	theme	Monitoring	1087:1096	arg1	data					1098:1101	Monitoring data	1087:1101	Monitoring data	1087:1101	Monitoring data demonstrated that differentially expressed bacterial strains were obtained until exposure for 14 days and 28 days, including increased L. gasseri, Turicibacter, and L. NK4A136_group and decreased Veillonella.
31738363	2	103	theme	environmental	317:329	arg1	exposure					331:338	environmental exposure	317:338	environmental exposure	317:338	Ingestion of TiO2 NPs via dietary and environmental exposure may pose potential health risks by interacting with gut microbiota.
31738363	9	104	theme	aminoacyl-tRNA	1375:1388	arg1	pathway					1413:1419	the aminoacyl-tRNA biosynthesis metabolic pathway	1371:1419	the aminoacyl-tRNA biosynthesis metabolic pathway	1371:1419	Fecal metabolomics analysis showed that 25 metabolites and the aminoacyl-tRNA biosynthesis metabolic pathway have changed significantly in exposed rats.
31738363	11	105	theme	gut	1701:1703	arg1	microbiota					1705:1714	gut microbiota	1701:1714	gut microbiota	1701:1714	Metabolic disorders of gut microbiota and subsequently produced lipopolysaccharides (LPS) led to oxidative stress and an inflammatory response in the intestine, which was considered to be a key and primary indirect pathway for toxicity induced by oral exposure to the TiO2 NPs.
31738363	1	106	theme	food	235:238	arg1	packaging					240:248	food packaging	235:248	food packaging	235:248	The antibacterial activity of titanium dioxide nanoparticles (TiO2 NPs) has been extensively documented and applied to food packaging or environmental protection.
31738363	14	107	theme	antimicrobial	2419:2431	arg1	activity					2433:2440	potential antimicrobial activity	2409:2440	potential antimicrobial activity	2409:2440	This may be a common mechanism of toxicity caused by oral administration of most nanomaterials, as they usually have potential antimicrobial activity.
31738363	9	108	theme	metabolic	1403:1411	arg1	pathway					1413:1419	the aminoacyl-tRNA biosynthesis metabolic pathway	1371:1419	the aminoacyl-tRNA biosynthesis metabolic pathway	1371:1419	Fecal metabolomics analysis showed that 25 metabolites and the aminoacyl-tRNA biosynthesis metabolic pathway have changed significantly in exposed rats.
31339480	4	0	dep	Aquibacillus	753:764	arg1	salifodinae					766:776	salifodinae	766:776	salifodinae	766:776	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain BH258T belongs to the genus Aquibacillus, showing highest sequence similarity to Aquibacillus koreensis BH30097T (96.1 %), Aquibacillus albus YIM 93624T (95.9 %), Aquibacillus halophilus B6BT (95.6 %) and Aquibacillus salifodinae WSY08-1T (95.1 %).
31339480	9	1	theme	name	1476:1479	arg1	sp					1504:1505	the name Aquibacillus sediminis sp	1472:1505	the name Aquibacillus sediminis sp	1472:1505	On the basis of the results of phylogenetic, phenotypic and chemotaxonomic analyses in this study, strain BH258T is considered to represent a novel species of the genus Aquibacillus, for which the name Aquibacillus sediminis sp.
31339480	4	2	theme	Phylogenetic	471:482	arg1	analyses					484:491	Phylogenetic analyses	471:491	Phylogenetic analyses based on 16S rRNA gene sequences	471:524	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain BH258T belongs to the genus Aquibacillus, showing highest sequence similarity to Aquibacillus koreensis BH30097T (96.1 %), Aquibacillus albus YIM 93624T (95.9 %), Aquibacillus halophilus B6BT (95.6 %) and Aquibacillus salifodinae WSY08-1T (95.1 %).
31339480	5	3	theme	meso-diaminopimelic	918:936	arg1	acid					969:972	the diagnostic diamino acid	946:972	the diagnostic diamino acid	946:972	The predominant isoprenoid quinone was identified as menaquinone-7, and the cell-wall peptidoglycan was found to contain meso-diaminopimelic acid as the diagnostic diamino acid.
31339480	5	3	theme	meso-diaminopimelic	918:936	arg1	acid					938:941	meso-diaminopimelic acid	918:941	meso-diaminopimelic acid	918:941	The predominant isoprenoid quinone was identified as menaquinone-7, and the cell-wall peptidoglycan was found to contain meso-diaminopimelic acid as the diagnostic diamino acid.
31339480	9	4	theme	strain	1378:1383	arg1	BH258T					1385:1390	strain BH258T	1378:1390	strain BH258T	1378:1390	On the basis of the results of phylogenetic, phenotypic and chemotaxonomic analyses in this study, strain BH258T is considered to represent a novel species of the genus Aquibacillus, for which the name Aquibacillus sediminis sp.
31339480	9	5	theme	Aquibacillus	1481:1492	arg1	sp					1504:1505	the name Aquibacillus sediminis sp	1472:1505	the name Aquibacillus sediminis sp	1472:1505	On the basis of the results of phylogenetic, phenotypic and chemotaxonomic analyses in this study, strain BH258T is considered to represent a novel species of the genus Aquibacillus, for which the name Aquibacillus sediminis sp.
31339480	2	6	theme	Gram-stain-positive	97:115	arg1	bacterium					140:148	A Gram-stain-positive, moderately halophilic bacterium	95:148	A Gram-stain-positive, moderately halophilic bacterium	95:148	A Gram-stain-positive, moderately halophilic bacterium, designated strain BH258T, was isolated from solar saltern sediment sampled at Shinan in the Republic of Korea.
31339480	8	7	theme	DNA	1208:1210	arg1	G+C content					1212:1222	The DNA G+C content	1204:1222	The DNA G+C content of this novel isolate	1204:1244	The DNA G+C content of this novel isolate was determined to be 37.35 mol%.
31339480	8	7	theme	DNA	1208:1210	arg1	%					1276:1276	37.35 mol%	1267:1276	37.35 mol%	1267:1276	The DNA G+C content of this novel isolate was determined to be 37.35 mol%.
31339480	9	8	theme	genus	1442:1446	arg1	Aquibacillus					1448:1459	the genus Aquibacillus	1438:1459	the genus Aquibacillus	1438:1459	On the basis of the results of phylogenetic, phenotypic and chemotaxonomic analyses in this study, strain BH258T is considered to represent a novel species of the genus Aquibacillus, for which the name Aquibacillus sediminis sp.
31339480	1	9	theme	halophilic	46:55	arg1	bacterium					57:65	a moderately halophilic bacterium	33:65	a moderately halophilic bacterium	33:65	nov., a moderately halophilic bacterium isolated from saltern soil.
31339480	1	9	theme	halophilic	46:55	arg1	nov.					27:30	nov.	27:30	nov.	27:30	nov., a moderately halophilic bacterium isolated from saltern soil.
31339480	8	10	theme	isolate	1238:1244	arg1	G+C content					1212:1222	The DNA G+C content	1204:1222	The DNA G+C content of this novel isolate	1204:1244	The DNA G+C content of this novel isolate was determined to be 37.35 mol%.
31339480	8	10	theme	isolate	1238:1244	arg1	%					1276:1276	37.35 mol%	1267:1276	37.35 mol%	1267:1276	The DNA G+C content of this novel isolate was determined to be 37.35 mol%.
31339480	4	11	theme	koreensis	642:650	arg1	BH30097T					652:659	Aquibacillus koreensis BH30097T	629:659	Aquibacillus koreensis BH30097T (96.1 %)	629:668	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain BH258T belongs to the genus Aquibacillus, showing highest sequence similarity to Aquibacillus koreensis BH30097T (96.1 %), Aquibacillus albus YIM 93624T (95.9 %), Aquibacillus halophilus B6BT (95.6 %) and Aquibacillus salifodinae WSY08-1T (95.1 %).
31339480	4	11	theme	koreensis	642:650	arg1	%					667:667	96.1 %	662:667	96.1 %	662:667	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain BH258T belongs to the genus Aquibacillus, showing highest sequence similarity to Aquibacillus koreensis BH30097T (96.1 %), Aquibacillus albus YIM 93624T (95.9 %), Aquibacillus halophilus B6BT (95.6 %) and Aquibacillus salifodinae WSY08-1T (95.1 %).
31339480	2	12	theme	strain	162:167	arg1	BH258T					169:174	strain BH258T	162:174	strain BH258T	162:174	A Gram-stain-positive, moderately halophilic bacterium, designated strain BH258T, was isolated from solar saltern sediment sampled at Shinan in the Republic of Korea.
31339480	3	13	theme	NaCl	457:460	arg1	salinities					428:437	salinities	428:437	salinities of 0.5-20 % (w/v) NaCl (7-10%)	428:468	Cells of strain BH258T were found to be strictly aerobic, motile, endospore-forming rods which could grow at 15-45 °C (optimum, 35 °C), at pH 5.5-9.0 (pH 7.0) and at salinities of 0.5-20 % (w/v) NaCl (7-10%).
31339480	6	14	theme	fatty	985:989	arg1	acids					991:995	The major fatty acids	975:995	The major fatty acids	975:995	The major fatty acids were identified as anteiso-C15 : 0, iso-C16 : 0, anteiso-C17 : 0 and iso-C15 : 0.
31339480	6	14	theme	fatty	985:989	arg1	anteiso-C15 					1016:1027	anteiso-C15 	1016:1027	anteiso-C15 	1016:1027	The major fatty acids were identified as anteiso-C15 : 0, iso-C16 : 0, anteiso-C17 : 0 and iso-C15 : 0.
31339480	4	15	theme	Aquibacillus	711:722	arg1	B6BT					735:738	Aquibacillus halophilus B6BT	711:738	Aquibacillus halophilus B6BT (95.6 %)	711:747	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain BH258T belongs to the genus Aquibacillus, showing highest sequence similarity to Aquibacillus koreensis BH30097T (96.1 %), Aquibacillus albus YIM 93624T (95.9 %), Aquibacillus halophilus B6BT (95.6 %) and Aquibacillus salifodinae WSY08-1T (95.1 %).
31339480	4	15	theme	Aquibacillus	711:722	arg1	%					746:746	95.6 %	741:746	95.6 %	741:746	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain BH258T belongs to the genus Aquibacillus, showing highest sequence similarity to Aquibacillus koreensis BH30097T (96.1 %), Aquibacillus albus YIM 93624T (95.9 %), Aquibacillus halophilus B6BT (95.6 %) and Aquibacillus salifodinae WSY08-1T (95.1 %).
31339480	9	16	theme	phylogenetic	1310:1321	arg1	analyses					1354:1361	phylogenetic, phenotypic and chemotaxonomic analyses	1310:1361	phylogenetic, phenotypic and chemotaxonomic analyses	1310:1361	On the basis of the results of phylogenetic, phenotypic and chemotaxonomic analyses in this study, strain BH258T is considered to represent a novel species of the genus Aquibacillus, for which the name Aquibacillus sediminis sp.
31339480	3	17	theme	w/v	452:454	arg1	NaCl					457:460	0.5-20 % (w/v) NaCl	442:460	0.5-20 % (w/v) NaCl (7-10%)	442:468	Cells of strain BH258T were found to be strictly aerobic, motile, endospore-forming rods which could grow at 15-45 °C (optimum, 35 °C), at pH 5.5-9.0 (pH 7.0) and at salinities of 0.5-20 % (w/v) NaCl (7-10%).
31339480	3	17	theme	w/v	452:454	arg1	%					467:467	7-10%	463:467	7-10%	463:467	Cells of strain BH258T were found to be strictly aerobic, motile, endospore-forming rods which could grow at 15-45 °C (optimum, 35 °C), at pH 5.5-9.0 (pH 7.0) and at salinities of 0.5-20 % (w/v) NaCl (7-10%).
31339480	0	18	theme	sediminis	13:21	arg1	sp					23:24	Aquibacillus sediminis sp	0:24	Aquibacillus sediminis sp.	0:25	Aquibacillus sediminis sp.
31339480	3	19	dep	optimum	381:387	arg1	35 °C					390:394	35 °C	390:394	35 °C	390:394	Cells of strain BH258T were found to be strictly aerobic, motile, endospore-forming rods which could grow at 15-45 °C (optimum, 35 °C), at pH 5.5-9.0 (pH 7.0) and at salinities of 0.5-20 % (w/v) NaCl (7-10%).
31339480	1	20	theme	saltern	81:87	arg1	soil					89:92	saltern soil	81:92	saltern soil	81:92	nov., a moderately halophilic bacterium isolated from saltern soil.
31339480	2	21	from	Shinan	229:234	arg1	Republic					243:250	the Republic	239:250	the Republic of Korea	239:259	A Gram-stain-positive, moderately halophilic bacterium, designated strain BH258T, was isolated from solar saltern sediment sampled at Shinan in the Republic of Korea.
31339480	6	22	dep	 0	1029:1030	arg1	iso-C15 					1066:1073	iso-C15 	1066:1073	iso-C15 	1066:1073	The major fatty acids were identified as anteiso-C15 : 0, iso-C16 : 0, anteiso-C17 : 0 and iso-C15 : 0.
31339480	6	22	dep	 0	1029:1030	arg1	 0					1059:1060	 0	1059:1060	 0	1059:1060	The major fatty acids were identified as anteiso-C15 : 0, iso-C16 : 0, anteiso-C17 : 0 and iso-C15 : 0.
31339480	6	22	dep	 0	1029:1030	arg1	 0					1042:1043	 0	1042:1043	 0	1042:1043	The major fatty acids were identified as anteiso-C15 : 0, iso-C16 : 0, anteiso-C17 : 0 and iso-C15 : 0.
31339480	6	22	dep	 0	1029:1030	arg1	 0					1075:1076	 0	1075:1076	 0	1075:1076	The major fatty acids were identified as anteiso-C15 : 0, iso-C16 : 0, anteiso-C17 : 0 and iso-C15 : 0.
31339480	0	23	theme	Aquibacillus	0:11	arg1	sp					23:24	Aquibacillus sediminis sp	0:24	Aquibacillus sediminis sp.	0:25	Aquibacillus sediminis sp.
31339480	11	24	theme	=KACC	1553:1557	arg1	BH258T					1545:1550	BH258T	1545:1550	BH258T (=KACC 18680T=NBRC 111875T)	1545:1578	The type strain is BH258T (=KACC 18680T=NBRC 111875T).
31339480	11	24	theme	=KACC	1553:1557	arg1	111875T					1571:1577	=KACC 18680T=NBRC 111875T	1553:1577	=KACC 18680T=NBRC 111875T	1553:1577	The type strain is BH258T (=KACC 18680T=NBRC 111875T).
31339480	4	25	theme	Aquibacillus	629:640	arg1	BH30097T					652:659	Aquibacillus koreensis BH30097T	629:659	Aquibacillus koreensis BH30097T (96.1 %)	629:668	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain BH258T belongs to the genus Aquibacillus, showing highest sequence similarity to Aquibacillus koreensis BH30097T (96.1 %), Aquibacillus albus YIM 93624T (95.9 %), Aquibacillus halophilus B6BT (95.6 %) and Aquibacillus salifodinae WSY08-1T (95.1 %).
31339480	4	25	theme	Aquibacillus	629:640	arg1	%					667:667	96.1 %	662:667	96.1 %	662:667	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain BH258T belongs to the genus Aquibacillus, showing highest sequence similarity to Aquibacillus koreensis BH30097T (96.1 %), Aquibacillus albus YIM 93624T (95.9 %), Aquibacillus halophilus B6BT (95.6 %) and Aquibacillus salifodinae WSY08-1T (95.1 %).
31339480	7	26	theme	unidentified	1176:1187	arg1	phospholipids					1189:1201	three unidentified phospholipids	1170:1201	three unidentified phospholipids	1170:1201	The major polar lipids were identified as phosphatidylglycerol, diphosphatidylglycerol and three unidentified phospholipids.
31339480	7	26	theme	unidentified	1176:1187	arg1	lipids					1095:1100	The major polar lipids	1079:1100	The major polar lipids	1079:1100	The major polar lipids were identified as phosphatidylglycerol, diphosphatidylglycerol and three unidentified phospholipids.
31339480	4	27	dep	Aquibacillus	711:722	arg1	halophilus					724:733	halophilus	724:733	halophilus	724:733	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain BH258T belongs to the genus Aquibacillus, showing highest sequence similarity to Aquibacillus koreensis BH30097T (96.1 %), Aquibacillus albus YIM 93624T (95.9 %), Aquibacillus halophilus B6BT (95.6 %) and Aquibacillus salifodinae WSY08-1T (95.1 %).
31339480	5	28	theme	predominant	801:811	arg1	menaquinone-7					850:862	menaquinone-7	850:862	menaquinone-7	850:862	The predominant isoprenoid quinone was identified as menaquinone-7, and the cell-wall peptidoglycan was found to contain meso-diaminopimelic acid as the diagnostic diamino acid.
31339480	5	28	theme	predominant	801:811	arg1	quinone					824:830	The predominant isoprenoid quinone	797:830	The predominant isoprenoid quinone	797:830	The predominant isoprenoid quinone was identified as menaquinone-7, and the cell-wall peptidoglycan was found to contain meso-diaminopimelic acid as the diagnostic diamino acid.
31339480	8	29	theme	novel	1232:1236	arg1	isolate					1238:1244	this novel isolate	1227:1244	this novel isolate	1227:1244	The DNA G+C content of this novel isolate was determined to be 37.35 mol%.
31339480	3	30	theme	strain	271:276	arg1	BH258T					278:283	strain BH258T	271:283	strain BH258T	271:283	Cells of strain BH258T were found to be strictly aerobic, motile, endospore-forming rods which could grow at 15-45 °C (optimum, 35 °C), at pH 5.5-9.0 (pH 7.0) and at salinities of 0.5-20 % (w/v) NaCl (7-10%).
31339480	11	31	theme	type	1530:1533	arg1	BH258T					1545:1550	BH258T	1545:1550	BH258T (=KACC 18680T=NBRC 111875T)	1545:1578	The type strain is BH258T (=KACC 18680T=NBRC 111875T).
31339480	11	31	theme	type	1530:1533	arg1	strain					1535:1540	The type strain	1526:1540	The type strain	1526:1540	The type strain is BH258T (=KACC 18680T=NBRC 111875T).
31339480	5	32	theme	isoprenoid	813:822	arg1	menaquinone-7					850:862	menaquinone-7	850:862	menaquinone-7	850:862	The predominant isoprenoid quinone was identified as menaquinone-7, and the cell-wall peptidoglycan was found to contain meso-diaminopimelic acid as the diagnostic diamino acid.
31339480	5	32	theme	isoprenoid	813:822	arg1	quinone					824:830	The predominant isoprenoid quinone	797:830	The predominant isoprenoid quinone	797:830	The predominant isoprenoid quinone was identified as menaquinone-7, and the cell-wall peptidoglycan was found to contain meso-diaminopimelic acid as the diagnostic diamino acid.
31339480	2	33	attach	isolated	181:188	arg2	bacterium					140:148	A Gram-stain-positive, moderately halophilic bacterium	95:148	A Gram-stain-positive, moderately halophilic bacterium	95:148	A Gram-stain-positive, moderately halophilic bacterium, designated strain BH258T, was isolated from solar saltern sediment sampled at Shinan in the Republic of Korea.
31339480	2	33	attach	isolated	181:188	arg1	sediment					209:216	solar saltern sediment	195:216	solar saltern sediment sampled at Shinan in the Republic of Korea	195:259	A Gram-stain-positive, moderately halophilic bacterium, designated strain BH258T, was isolated from solar saltern sediment sampled at Shinan in the Republic of Korea.
31339480	9	34	theme	sediminis	1494:1502	arg1	sp					1504:1505	the name Aquibacillus sediminis sp	1472:1505	the name Aquibacillus sediminis sp	1472:1505	On the basis of the results of phylogenetic, phenotypic and chemotaxonomic analyses in this study, strain BH258T is considered to represent a novel species of the genus Aquibacillus, for which the name Aquibacillus sediminis sp.
31339480	9	35	theme	chemotaxonomic	1339:1352	arg1	analyses					1354:1361	phylogenetic, phenotypic and chemotaxonomic analyses	1310:1361	phylogenetic, phenotypic and chemotaxonomic analyses	1310:1361	On the basis of the results of phylogenetic, phenotypic and chemotaxonomic analyses in this study, strain BH258T is considered to represent a novel species of the genus Aquibacillus, for which the name Aquibacillus sediminis sp.
31339480	3	36	dep	aerobic	311:317	arg1	endospore-forming					328:344	endospore-forming	328:344	endospore-forming	328:344	Cells of strain BH258T were found to be strictly aerobic, motile, endospore-forming rods which could grow at 15-45 °C (optimum, 35 °C), at pH 5.5-9.0 (pH 7.0) and at salinities of 0.5-20 % (w/v) NaCl (7-10%).
31339480	3	36	dep	aerobic	311:317	arg1	motile					320:325	motile	320:325	motile	320:325	Cells of strain BH258T were found to be strictly aerobic, motile, endospore-forming rods which could grow at 15-45 °C (optimum, 35 °C), at pH 5.5-9.0 (pH 7.0) and at salinities of 0.5-20 % (w/v) NaCl (7-10%).
31339480	7	37	theme	major	1083:1087	arg1	diphosphatidylglycerol					1143:1164	diphosphatidylglycerol	1143:1164	diphosphatidylglycerol	1143:1164	The major polar lipids were identified as phosphatidylglycerol, diphosphatidylglycerol and three unidentified phospholipids.
31339480	7	37	theme	major	1083:1087	arg1	phosphatidylglycerol					1121:1140	phosphatidylglycerol	1121:1140	phosphatidylglycerol	1121:1140	The major polar lipids were identified as phosphatidylglycerol, diphosphatidylglycerol and three unidentified phospholipids.
31339480	7	37	theme	major	1083:1087	arg1	phospholipids					1189:1201	three unidentified phospholipids	1170:1201	three unidentified phospholipids	1170:1201	The major polar lipids were identified as phosphatidylglycerol, diphosphatidylglycerol and three unidentified phospholipids.
31339480	7	37	theme	major	1083:1087	arg1	lipids					1095:1100	The major polar lipids	1079:1100	The major polar lipids	1079:1100	The major polar lipids were identified as phosphatidylglycerol, diphosphatidylglycerol and three unidentified phospholipids.
31339480	5	38	theme	cell-wall	873:881	arg1	peptidoglycan					883:895	the cell-wall peptidoglycan	869:895	the cell-wall peptidoglycan	869:895	The predominant isoprenoid quinone was identified as menaquinone-7, and the cell-wall peptidoglycan was found to contain meso-diaminopimelic acid as the diagnostic diamino acid.
31339480	4	39	theme	strain	541:546	arg1	BH258T					548:553	strain BH258T	541:553	strain BH258T	541:553	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain BH258T belongs to the genus Aquibacillus, showing highest sequence similarity to Aquibacillus koreensis BH30097T (96.1 %), Aquibacillus albus YIM 93624T (95.9 %), Aquibacillus halophilus B6BT (95.6 %) and Aquibacillus salifodinae WSY08-1T (95.1 %).
31339480	5	40	theme	diagnostic	950:959	arg1	acid					969:972	the diagnostic diamino acid	946:972	the diagnostic diamino acid	946:972	The predominant isoprenoid quinone was identified as menaquinone-7, and the cell-wall peptidoglycan was found to contain meso-diaminopimelic acid as the diagnostic diamino acid.
31339480	5	40	theme	diagnostic	950:959	arg1	acid					938:941	meso-diaminopimelic acid	918:941	meso-diaminopimelic acid	918:941	The predominant isoprenoid quinone was identified as menaquinone-7, and the cell-wall peptidoglycan was found to contain meso-diaminopimelic acid as the diagnostic diamino acid.
31339480	9	41	dep	results	1299:1305	arg1	basis					1286:1290	basis	1286:1290	basis	1286:1290	On the basis of the results of phylogenetic, phenotypic and chemotaxonomic analyses in this study, strain BH258T is considered to represent a novel species of the genus Aquibacillus, for which the name Aquibacillus sediminis sp.
31339480	9	41	dep	results	1299:1305	arg1	the					1282:1284	the	1282:1284	the	1282:1284	On the basis of the results of phylogenetic, phenotypic and chemotaxonomic analyses in this study, strain BH258T is considered to represent a novel species of the genus Aquibacillus, for which the name Aquibacillus sediminis sp.
31339480	9	42	theme	phenotypic	1324:1333	arg1	analyses					1354:1361	phylogenetic, phenotypic and chemotaxonomic analyses	1310:1361	phylogenetic, phenotypic and chemotaxonomic analyses	1310:1361	On the basis of the results of phylogenetic, phenotypic and chemotaxonomic analyses in this study, strain BH258T is considered to represent a novel species of the genus Aquibacillus, for which the name Aquibacillus sediminis sp.
31339480	5	43	contain	contain	910:916	arg1	peptidoglycan					883:895	the cell-wall peptidoglycan	869:895	the cell-wall peptidoglycan	869:895	The predominant isoprenoid quinone was identified as menaquinone-7, and the cell-wall peptidoglycan was found to contain meso-diaminopimelic acid as the diagnostic diamino acid.
31339480	5	43	contain	contain	910:916	arg2	acid					938:941	meso-diaminopimelic acid	918:941	meso-diaminopimelic acid	918:941	The predominant isoprenoid quinone was identified as menaquinone-7, and the cell-wall peptidoglycan was found to contain meso-diaminopimelic acid as the diagnostic diamino acid.
31339480	5	43	contain	contain	910:916	arg2	acid					969:972	the diagnostic diamino acid	946:972	the diagnostic diamino acid	946:972	The predominant isoprenoid quinone was identified as menaquinone-7, and the cell-wall peptidoglycan was found to contain meso-diaminopimelic acid as the diagnostic diamino acid.
31339480	2	44	dep	Gram-stain-positive	97:115	arg1	halophilic					129:138	halophilic	129:138	halophilic	129:138	A Gram-stain-positive, moderately halophilic bacterium, designated strain BH258T, was isolated from solar saltern sediment sampled at Shinan in the Republic of Korea.
31339480	4	45	theme	Aquibacillus	753:764	arg1	WSY08-1T					778:785	Aquibacillus salifodinae WSY08-1T	753:785	Aquibacillus salifodinae WSY08-1T (95.1 %)	753:794	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain BH258T belongs to the genus Aquibacillus, showing highest sequence similarity to Aquibacillus koreensis BH30097T (96.1 %), Aquibacillus albus YIM 93624T (95.9 %), Aquibacillus halophilus B6BT (95.6 %) and Aquibacillus salifodinae WSY08-1T (95.1 %).
31339480	4	45	theme	Aquibacillus	753:764	arg1	%					793:793	95.1 %	788:793	95.1 %	788:793	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain BH258T belongs to the genus Aquibacillus, showing highest sequence similarity to Aquibacillus koreensis BH30097T (96.1 %), Aquibacillus albus YIM 93624T (95.9 %), Aquibacillus halophilus B6BT (95.6 %) and Aquibacillus salifodinae WSY08-1T (95.1 %).
31339480	5	46	theme	diamino	961:967	arg1	acid					969:972	the diagnostic diamino acid	946:972	the diagnostic diamino acid	946:972	The predominant isoprenoid quinone was identified as menaquinone-7, and the cell-wall peptidoglycan was found to contain meso-diaminopimelic acid as the diagnostic diamino acid.
31339480	5	46	theme	diamino	961:967	arg1	acid					938:941	meso-diaminopimelic acid	918:941	meso-diaminopimelic acid	918:941	The predominant isoprenoid quinone was identified as menaquinone-7, and the cell-wall peptidoglycan was found to contain meso-diaminopimelic acid as the diagnostic diamino acid.
31339480	8	47	theme	37.35 mol	1267:1275	arg1	G+C content					1212:1222	The DNA G+C content	1204:1222	The DNA G+C content of this novel isolate	1204:1244	The DNA G+C content of this novel isolate was determined to be 37.35 mol%.
31339480	8	47	theme	37.35 mol	1267:1275	arg1	%					1276:1276	37.35 mol%	1267:1276	37.35 mol%	1267:1276	The DNA G+C content of this novel isolate was determined to be 37.35 mol%.
31339480	11	48	theme	18680T=NBRC	1559:1569	arg1	BH258T					1545:1550	BH258T	1545:1550	BH258T (=KACC 18680T=NBRC 111875T)	1545:1578	The type strain is BH258T (=KACC 18680T=NBRC 111875T).
31339480	11	48	theme	18680T=NBRC	1559:1569	arg1	111875T					1571:1577	=KACC 18680T=NBRC 111875T	1553:1577	=KACC 18680T=NBRC 111875T	1553:1577	The type strain is BH258T (=KACC 18680T=NBRC 111875T).
31339480	4	49	theme	sequence	606:613	arg1	similarity					615:624	highest sequence similarity	598:624	highest sequence similarity to Aquibacillus koreensis BH30097T (96.1 %), Aquibacillus albus YIM 93624T (95.9 %), Aquibacillus halophilus B6BT (95.6 %) and Aquibacillus salifodinae WSY08-1T (95.1 %)	598:794	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain BH258T belongs to the genus Aquibacillus, showing highest sequence similarity to Aquibacillus koreensis BH30097T (96.1 %), Aquibacillus albus YIM 93624T (95.9 %), Aquibacillus halophilus B6BT (95.6 %) and Aquibacillus salifodinae WSY08-1T (95.1 %).
31339480	9	50	theme	Aquibacillus	1448:1459	arg1	species					1427:1433	a novel species	1419:1433	a novel species	1419:1433	On the basis of the results of phylogenetic, phenotypic and chemotaxonomic analyses in this study, strain BH258T is considered to represent a novel species of the genus Aquibacillus, for which the name Aquibacillus sediminis sp.
31339480	4	51	theme	highest	598:604	arg1	similarity					615:624	highest sequence similarity	598:624	highest sequence similarity to Aquibacillus koreensis BH30097T (96.1 %), Aquibacillus albus YIM 93624T (95.9 %), Aquibacillus halophilus B6BT (95.6 %) and Aquibacillus salifodinae WSY08-1T (95.1 %)	598:794	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain BH258T belongs to the genus Aquibacillus, showing highest sequence similarity to Aquibacillus koreensis BH30097T (96.1 %), Aquibacillus albus YIM 93624T (95.9 %), Aquibacillus halophilus B6BT (95.6 %) and Aquibacillus salifodinae WSY08-1T (95.1 %).
31339480	7	52	theme	polar	1089:1093	arg1	diphosphatidylglycerol					1143:1164	diphosphatidylglycerol	1143:1164	diphosphatidylglycerol	1143:1164	The major polar lipids were identified as phosphatidylglycerol, diphosphatidylglycerol and three unidentified phospholipids.
31339480	7	52	theme	polar	1089:1093	arg1	phosphatidylglycerol					1121:1140	phosphatidylglycerol	1121:1140	phosphatidylglycerol	1121:1140	The major polar lipids were identified as phosphatidylglycerol, diphosphatidylglycerol and three unidentified phospholipids.
31339480	7	52	theme	polar	1089:1093	arg1	phospholipids					1189:1201	three unidentified phospholipids	1170:1201	three unidentified phospholipids	1170:1201	The major polar lipids were identified as phosphatidylglycerol, diphosphatidylglycerol and three unidentified phospholipids.
31339480	7	52	theme	polar	1089:1093	arg1	lipids					1095:1100	The major polar lipids	1079:1100	The major polar lipids	1079:1100	The major polar lipids were identified as phosphatidylglycerol, diphosphatidylglycerol and three unidentified phospholipids.
31339480	3	53	theme	BH258T	278:283	arg1	Cells					262:266	Cells	262:266	Cells of strain BH258T	262:283	Cells of strain BH258T were found to be strictly aerobic, motile, endospore-forming rods which could grow at 15-45 °C (optimum, 35 °C), at pH 5.5-9.0 (pH 7.0) and at salinities of 0.5-20 % (w/v) NaCl (7-10%).
31339480	2	54	theme	saltern	201:207	arg1	sediment					209:216	solar saltern sediment	195:216	solar saltern sediment sampled at Shinan in the Republic of Korea	195:259	A Gram-stain-positive, moderately halophilic bacterium, designated strain BH258T, was isolated from solar saltern sediment sampled at Shinan in the Republic of Korea.
31339480	2	55	theme	Korea	255:259	arg1	Republic					243:250	the Republic	239:250	the Republic of Korea	239:259	A Gram-stain-positive, moderately halophilic bacterium, designated strain BH258T, was isolated from solar saltern sediment sampled at Shinan in the Republic of Korea.
31339480	9	56	theme	analyses	1354:1361	arg1	results					1299:1305	the results	1295:1305	the results of phylogenetic, phenotypic and chemotaxonomic analyses in this study	1295:1375	On the basis of the results of phylogenetic, phenotypic and chemotaxonomic analyses in this study, strain BH258T is considered to represent a novel species of the genus Aquibacillus, for which the name Aquibacillus sediminis sp.
31339480	9	57	theme	novel	1421:1425	arg1	species					1427:1433	a novel species	1419:1433	a novel species	1419:1433	On the basis of the results of phylogenetic, phenotypic and chemotaxonomic analyses in this study, strain BH258T is considered to represent a novel species of the genus Aquibacillus, for which the name Aquibacillus sediminis sp.
31339480	4	58	theme	16S	502:504	arg1	sequences					516:524	16S rRNA gene sequences	502:524	16S rRNA gene sequences	502:524	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain BH258T belongs to the genus Aquibacillus, showing highest sequence similarity to Aquibacillus koreensis BH30097T (96.1 %), Aquibacillus albus YIM 93624T (95.9 %), Aquibacillus halophilus B6BT (95.6 %) and Aquibacillus salifodinae WSY08-1T (95.1 %).
31339480	2	59	theme	solar	195:199	arg1	sediment					209:216	solar saltern sediment	195:216	solar saltern sediment sampled at Shinan in the Republic of Korea	195:259	A Gram-stain-positive, moderately halophilic bacterium, designated strain BH258T, was isolated from solar saltern sediment sampled at Shinan in the Republic of Korea.
31339480	4	60	theme	Aquibacillus	671:682	arg1	93624T					694:699	Aquibacillus albus YIM 93624T	671:699	Aquibacillus albus YIM 93624T (95.9 %)	671:708	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain BH258T belongs to the genus Aquibacillus, showing highest sequence similarity to Aquibacillus koreensis BH30097T (96.1 %), Aquibacillus albus YIM 93624T (95.9 %), Aquibacillus halophilus B6BT (95.6 %) and Aquibacillus salifodinae WSY08-1T (95.1 %).
31339480	4	60	theme	Aquibacillus	671:682	arg1	%					707:707	95.9 %	702:707	95.9 %	702:707	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain BH258T belongs to the genus Aquibacillus, showing highest sequence similarity to Aquibacillus koreensis BH30097T (96.1 %), Aquibacillus albus YIM 93624T (95.9 %), Aquibacillus halophilus B6BT (95.6 %) and Aquibacillus salifodinae WSY08-1T (95.1 %).
31339480	4	61	theme	genus	570:574	arg1	Aquibacillus					576:587	the genus Aquibacillus	566:587	the genus Aquibacillus	566:587	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain BH258T belongs to the genus Aquibacillus, showing highest sequence similarity to Aquibacillus koreensis BH30097T (96.1 %), Aquibacillus albus YIM 93624T (95.9 %), Aquibacillus halophilus B6BT (95.6 %) and Aquibacillus salifodinae WSY08-1T (95.1 %).
31339480	4	62	theme	albus	684:688	arg1	93624T					694:699	Aquibacillus albus YIM 93624T	671:699	Aquibacillus albus YIM 93624T (95.9 %)	671:708	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain BH258T belongs to the genus Aquibacillus, showing highest sequence similarity to Aquibacillus koreensis BH30097T (96.1 %), Aquibacillus albus YIM 93624T (95.9 %), Aquibacillus halophilus B6BT (95.6 %) and Aquibacillus salifodinae WSY08-1T (95.1 %).
31339480	4	62	theme	albus	684:688	arg1	%					707:707	95.9 %	702:707	95.9 %	702:707	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain BH258T belongs to the genus Aquibacillus, showing highest sequence similarity to Aquibacillus koreensis BH30097T (96.1 %), Aquibacillus albus YIM 93624T (95.9 %), Aquibacillus halophilus B6BT (95.6 %) and Aquibacillus salifodinae WSY08-1T (95.1 %).
31339480	3	63	theme	aerobic	311:317	arg1	rods					346:349	aerobic, motile, endospore-forming rods	311:349	aerobic, motile, endospore-forming rods which could grow at 15-45 °C (optimum, 35 °C), at pH 5.5-9.0 (pH 7.0) and at salinities of 0.5-20 % (w/v) NaCl (7-10%)	311:468	Cells of strain BH258T were found to be strictly aerobic, motile, endospore-forming rods which could grow at 15-45 °C (optimum, 35 °C), at pH 5.5-9.0 (pH 7.0) and at salinities of 0.5-20 % (w/v) NaCl (7-10%).
31339480	6	64	dep	identified	1002:1011	arg1	 0					1029:1030	 0	1029:1030	 0	1029:1030	The major fatty acids were identified as anteiso-C15 : 0, iso-C16 : 0, anteiso-C17 : 0 and iso-C15 : 0.
31339480	9	65	from	results	1299:1305	arg1	study					1371:1375	this study	1366:1375	this study	1366:1375	On the basis of the results of phylogenetic, phenotypic and chemotaxonomic analyses in this study, strain BH258T is considered to represent a novel species of the genus Aquibacillus, for which the name Aquibacillus sediminis sp.
31339480	6	66	theme	major	979:983	arg1	acids					991:995	The major fatty acids	975:995	The major fatty acids	975:995	The major fatty acids were identified as anteiso-C15 : 0, iso-C16 : 0, anteiso-C17 : 0 and iso-C15 : 0.
31339480	6	66	theme	major	979:983	arg1	anteiso-C15 					1016:1027	anteiso-C15 	1016:1027	anteiso-C15 	1016:1027	The major fatty acids were identified as anteiso-C15 : 0, iso-C16 : 0, anteiso-C17 : 0 and iso-C15 : 0.
31339480	4	67	theme	gene	511:514	arg1	sequences					516:524	16S rRNA gene sequences	502:524	16S rRNA gene sequences	502:524	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain BH258T belongs to the genus Aquibacillus, showing highest sequence similarity to Aquibacillus koreensis BH30097T (96.1 %), Aquibacillus albus YIM 93624T (95.9 %), Aquibacillus halophilus B6BT (95.6 %) and Aquibacillus salifodinae WSY08-1T (95.1 %).
31339480	4	68	theme	YIM	690:692	arg1	93624T					694:699	Aquibacillus albus YIM 93624T	671:699	Aquibacillus albus YIM 93624T (95.9 %)	671:708	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain BH258T belongs to the genus Aquibacillus, showing highest sequence similarity to Aquibacillus koreensis BH30097T (96.1 %), Aquibacillus albus YIM 93624T (95.9 %), Aquibacillus halophilus B6BT (95.6 %) and Aquibacillus salifodinae WSY08-1T (95.1 %).
31339480	4	68	theme	YIM	690:692	arg1	%					707:707	95.9 %	702:707	95.9 %	702:707	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain BH258T belongs to the genus Aquibacillus, showing highest sequence similarity to Aquibacillus koreensis BH30097T (96.1 %), Aquibacillus albus YIM 93624T (95.9 %), Aquibacillus halophilus B6BT (95.6 %) and Aquibacillus salifodinae WSY08-1T (95.1 %).
31339480	3	69	theme	%	449:449	arg1	NaCl					457:460	0.5-20 % (w/v) NaCl	442:460	0.5-20 % (w/v) NaCl (7-10%)	442:468	Cells of strain BH258T were found to be strictly aerobic, motile, endospore-forming rods which could grow at 15-45 °C (optimum, 35 °C), at pH 5.5-9.0 (pH 7.0) and at salinities of 0.5-20 % (w/v) NaCl (7-10%).
31339480	3	69	theme	%	449:449	arg1	%					467:467	7-10%	463:467	7-10%	463:467	Cells of strain BH258T were found to be strictly aerobic, motile, endospore-forming rods which could grow at 15-45 °C (optimum, 35 °C), at pH 5.5-9.0 (pH 7.0) and at salinities of 0.5-20 % (w/v) NaCl (7-10%).
31339480	4	70	theme	rRNA	506:509	arg1	sequences					516:524	16S rRNA gene sequences	502:524	16S rRNA gene sequences	502:524	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain BH258T belongs to the genus Aquibacillus, showing highest sequence similarity to Aquibacillus koreensis BH30097T (96.1 %), Aquibacillus albus YIM 93624T (95.9 %), Aquibacillus halophilus B6BT (95.6 %) and Aquibacillus salifodinae WSY08-1T (95.1 %).
30465644	2	0	theme	Gram-stain-positive	102:120	arg1	actinobacterium					177:191	A Gram-stain-positive, aerobic, non-motile, non-spore-forming, coccus-shaped actinobacterium	100:191	A Gram-stain-positive, aerobic, non-motile, non-spore-forming, coccus-shaped actinobacterium	100:191	A Gram-stain-positive, aerobic, non-motile, non-spore-forming, coccus-shaped actinobacterium, designated strain 12Sc4-1T, was isolated from a soil sample collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, China.
30465644	10	1	theme	phylogenetic	1413:1424	arg1	analyses					1457:1464	phylogenetic, phenotypic and chemotaxonomic analyses	1413:1464	phylogenetic, phenotypic and chemotaxonomic analyses	1413:1464	On the basis of phylogenetic, phenotypic and chemotaxonomic analyses, strain 12Sc4-1T represents a novel species of the genus Nakamurella, for which the name Nakamurelladeserti sp.
30465644	6	2	theme	Chemotaxonomic	818:831	arg1	analyses					833:840	Chemotaxonomic analyses	818:840	Chemotaxonomic analyses	818:840	Chemotaxonomic analyses showed that the isolate possessed meso-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan, glucose, mannose and galactose as whole-cell sugars, and MK-8(H4) as the predominant menaquinone.
30465644	2	3	attach	isolated	226:233	arg1	sample					247:252	a soil sample	240:252	a soil sample collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, China	240:330	A Gram-stain-positive, aerobic, non-motile, non-spore-forming, coccus-shaped actinobacterium, designated strain 12Sc4-1T, was isolated from a soil sample collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, China.
30465644	2	3	attach	isolated	226:233	arg2	actinobacterium					177:191	A Gram-stain-positive, aerobic, non-motile, non-spore-forming, coccus-shaped actinobacterium	100:191	A Gram-stain-positive, aerobic, non-motile, non-spore-forming, coccus-shaped actinobacterium	100:191	A Gram-stain-positive, aerobic, non-motile, non-spore-forming, coccus-shaped actinobacterium, designated strain 12Sc4-1T, was isolated from a soil sample collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, China.
30465644	10	4	theme	chemotaxonomic	1442:1455	arg1	analyses					1457:1464	phylogenetic, phenotypic and chemotaxonomic analyses	1413:1464	phylogenetic, phenotypic and chemotaxonomic analyses	1413:1464	On the basis of phylogenetic, phenotypic and chemotaxonomic analyses, strain 12Sc4-1T represents a novel species of the genus Nakamurella, for which the name Nakamurelladeserti sp.
30465644	3	5	theme	%	442:442	arg1	optimum					456:462	optimum	456:462	optimum	456:462	Strain 12Sc4-1T grew at 8‒35 °C (optimum, 28‒30 °C), pH 6.0‒9.0 (optimum, pH 7.0) and in the presence of 0‒3 % (w/v) NaCl (optimum, 0 %).
30465644	3	5	theme	%	442:442	arg1	NaCl					450:453	0‒3 % (w/v) NaCl	438:453	0‒3 % (w/v) NaCl (optimum, 0 %)	438:468	Strain 12Sc4-1T grew at 8‒35 °C (optimum, 28‒30 °C), pH 6.0‒9.0 (optimum, pH 7.0) and in the presence of 0‒3 % (w/v) NaCl (optimum, 0 %).
30465644	9	6	theme	DNA	1342:1344	arg1	G+C content					1346:1356	The DNA G+C content	1338:1356	The DNA G+C content	1338:1356	The DNA G+C content was 72.1 mol% (draft genome sequence).
30465644	9	6	theme	DNA	1342:1344	arg1	%					1370:1370	72.1 mol%	1362:1370	72.1 mol% (draft genome sequence)	1362:1394	The DNA G+C content was 72.1 mol% (draft genome sequence).
30465644	10	7	theme	novel	1496:1500	arg1	species					1502:1508	a novel species	1494:1508	a novel species	1494:1508	On the basis of phylogenetic, phenotypic and chemotaxonomic analyses, strain 12Sc4-1T represents a novel species of the genus Nakamurella, for which the name Nakamurelladeserti sp.
30465644	3	8	dep	optimum	366:372	arg1	28‒30 °C					375:382	28‒30 °C	375:382	28‒30 °C	375:382	Strain 12Sc4-1T grew at 8‒35 °C (optimum, 28‒30 °C), pH 6.0‒9.0 (optimum, pH 7.0) and in the presence of 0‒3 % (w/v) NaCl (optimum, 0 %).
30465644	6	9	contain	possessed	866:874	arg1	isolate					858:864	the isolate	854:864	the isolate	854:864	Chemotaxonomic analyses showed that the isolate possessed meso-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan, glucose, mannose and galactose as whole-cell sugars, and MK-8(H4) as the predominant menaquinone.
30465644	6	9	contain	possessed	866:874	arg2	acid					927:930	the diagnostic diamino acid	904:930	the diagnostic diamino acid of the peptidoglycan	904:951	Chemotaxonomic analyses showed that the isolate possessed meso-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan, glucose, mannose and galactose as whole-cell sugars, and MK-8(H4) as the predominant menaquinone.
30465644	6	9	contain	possessed	866:874	arg2	menaquinone					1039:1049	the predominant menaquinone	1023:1049	the predominant menaquinone	1023:1049	Chemotaxonomic analyses showed that the isolate possessed meso-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan, glucose, mannose and galactose as whole-cell sugars, and MK-8(H4) as the predominant menaquinone.
30465644	6	9	contain	possessed	866:874	arg2	acid					896:899	meso-diaminopimelic acid	876:899	meso-diaminopimelic acid	876:899	Chemotaxonomic analyses showed that the isolate possessed meso-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan, glucose, mannose and galactose as whole-cell sugars, and MK-8(H4) as the predominant menaquinone.
30465644	1	10	theme	rhizosphere	44:54	arg1	soil					56:59	rhizosphere soil	44:59	rhizosphere soil of Reaumuria in the Taklamakan desert	44:97	nov., isolated from rhizosphere soil of Reaumuria in the Taklamakan desert.
30465644	1	11	from	soil	56:59	arg1	desert					92:97	the Taklamakan desert	77:97	the Taklamakan desert	77:97	nov., isolated from rhizosphere soil of Reaumuria in the Taklamakan desert.
30465644	4	12	theme	sequence	627:634	arg1	similarity					636:645	the highest 16S rRNA gene sequence similarity	601:645	the highest 16S rRNA gene sequence similarity to Nakamurella silvestris S20-107T	601:680	Phylogenetic analysis based on 16S rRNA gene sequence suggested that strain 12Sc4-1T belonged to the genus Nakamurella and shared the highest 16S rRNA gene sequence similarity to Nakamurella silvestris S20-107T (96.94 %).
30465644	6	13	theme	predominant	1027:1037	arg1	acid					896:899	meso-diaminopimelic acid	876:899	meso-diaminopimelic acid	876:899	Chemotaxonomic analyses showed that the isolate possessed meso-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan, glucose, mannose and galactose as whole-cell sugars, and MK-8(H4) as the predominant menaquinone.
30465644	6	13	theme	predominant	1027:1037	arg1	menaquinone					1039:1049	the predominant menaquinone	1023:1049	the predominant menaquinone	1023:1049	Chemotaxonomic analyses showed that the isolate possessed meso-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan, glucose, mannose and galactose as whole-cell sugars, and MK-8(H4) as the predominant menaquinone.
30465644	8	14	theme	major	1286:1290	arg1	acids					1298:1302	The major fatty acids	1282:1302	The major fatty acids	1282:1302	The major fatty acids were C16 : 0 and anteiso-C15 : 0.
30465644	8	14	theme	major	1286:1290	arg1	C16 					1309:1312	C16 	1309:1312	C16 	1309:1312	The major fatty acids were C16 : 0 and anteiso-C15 : 0.
30465644	2	15	theme	Taklamakan	271:280	arg1	desert					282:287	the Taklamakan desert	267:287	the Taklamakan desert	267:287	A Gram-stain-positive, aerobic, non-motile, non-spore-forming, coccus-shaped actinobacterium, designated strain 12Sc4-1T, was isolated from a soil sample collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, China.
30465644	3	16	dep	optimum	456:462	arg1	%					467:467	0 %	465:467	0 %	465:467	Strain 12Sc4-1T grew at 8‒35 °C (optimum, 28‒30 °C), pH 6.0‒9.0 (optimum, pH 7.0) and in the presence of 0‒3 % (w/v) NaCl (optimum, 0 %).
30465644	10	17	theme	genus	1517:1521	arg1	Nakamurella					1523:1533	the genus Nakamurella	1513:1533	the genus Nakamurella	1513:1533	On the basis of phylogenetic, phenotypic and chemotaxonomic analyses, strain 12Sc4-1T represents a novel species of the genus Nakamurella, for which the name Nakamurelladeserti sp.
30465644	5	18	theme	Strain	693:698	arg1	12Sc4-1T					700:707	Strain 12Sc4-1T	693:707	Strain 12Sc4-1T	693:707	Strain 12Sc4-1T showed <96.0 % 16S rRNA gene sequence similarities to all other recognized members of the genus Nakamurella.
30465644	10	19	theme	name	1550:1553	arg1	sp					1574:1575	the name Nakamurelladeserti sp	1546:1575	the name Nakamurelladeserti sp	1546:1575	On the basis of phylogenetic, phenotypic and chemotaxonomic analyses, strain 12Sc4-1T represents a novel species of the genus Nakamurella, for which the name Nakamurelladeserti sp.
30465644	5	20	theme	sequence	738:745	arg1	similarities					747:758	<96.0 % 16S rRNA gene sequence similarities	716:758	<96.0 % 16S rRNA gene sequence similarities to all other recognized members of the genus Nakamurella	716:815	Strain 12Sc4-1T showed <96.0 % 16S rRNA gene sequence similarities to all other recognized members of the genus Nakamurella.
30465644	8	21	theme	fatty	1292:1296	arg1	acids					1298:1302	The major fatty acids	1282:1302	The major fatty acids	1282:1302	The major fatty acids were C16 : 0 and anteiso-C15 : 0.
30465644	8	21	theme	fatty	1292:1296	arg1	C16 					1309:1312	C16 	1309:1312	C16 	1309:1312	The major fatty acids were C16 : 0 and anteiso-C15 : 0.
30465644	4	22	theme	Phylogenetic	471:482	arg1	analysis					484:491	Phylogenetic analysis	471:491	Phylogenetic analysis based on 16S rRNA gene sequence	471:523	Phylogenetic analysis based on 16S rRNA gene sequence suggested that strain 12Sc4-1T belonged to the genus Nakamurella and shared the highest 16S rRNA gene sequence similarity to Nakamurella silvestris S20-107T (96.94 %).
30465644	1	23	theme	Reaumuria	64:72	arg1	soil					56:59	rhizosphere soil	44:59	rhizosphere soil of Reaumuria in the Taklamakan desert	44:97	nov., isolated from rhizosphere soil of Reaumuria in the Taklamakan desert.
30465644	10	24	theme	Nakamurelladeserti	1555:1572	arg1	sp					1574:1575	the name Nakamurelladeserti sp	1546:1575	the name Nakamurelladeserti sp	1546:1575	On the basis of phylogenetic, phenotypic and chemotaxonomic analyses, strain 12Sc4-1T represents a novel species of the genus Nakamurella, for which the name Nakamurelladeserti sp.
30465644	0	25	theme	deserti	12:18	arg1	sp					20:21	Nakamurella deserti sp	0:21	Nakamurella deserti sp.	0:22	Nakamurella deserti sp.
30465644	7	26	theme	unidentified	1219:1230	arg1	phosphoglycolipid					1232:1248	an unidentified phosphoglycolipid	1216:1248	an unidentified phosphoglycolipid	1216:1248	The polar lipids comprised diphosphatidylglycerol, phosphatidylethanolamine, an unidentified aminophospholipid, phosphatidylinositol, an unidentified phospholipid, an unidentified phosphoglycolipid and an unidentified glycolipid.
30465644	12	27	theme	49114T=CGMCC	1631:1642	arg1	12Sc4-1T					1615:1622	12Sc4-1T	1615:1622	12Sc4-1T (=KCTC 49114T=CGMCC 1.16555T)	1615:1652	The type strain is 12Sc4-1T (=KCTC 49114T=CGMCC 1.16555T).
30465644	12	27	theme	49114T=CGMCC	1631:1642	arg1	1.16555T					1644:1651	=KCTC 49114T=CGMCC 1.16555T	1625:1651	=KCTC 49114T=CGMCC 1.16555T	1625:1651	The type strain is 12Sc4-1T (=KCTC 49114T=CGMCC 1.16555T).
30465644	2	28	theme	Autonomous	307:316	arg1	Region					318:323	Autonomous Region	307:323	Autonomous Region	307:323	A Gram-stain-positive, aerobic, non-motile, non-spore-forming, coccus-shaped actinobacterium, designated strain 12Sc4-1T, was isolated from a soil sample collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, China.
30465644	7	29	theme	polar	1056:1060	arg1	lipids					1062:1067	The polar lipids	1052:1067	The polar lipids	1052:1067	The polar lipids comprised diphosphatidylglycerol, phosphatidylethanolamine, an unidentified aminophospholipid, phosphatidylinositol, an unidentified phospholipid, an unidentified phosphoglycolipid and an unidentified glycolipid.
30465644	7	30	theme	unidentified	1257:1268	arg1	glycolipid					1270:1279	an unidentified glycolipid	1254:1279	an unidentified glycolipid	1254:1279	The polar lipids comprised diphosphatidylglycerol, phosphatidylethanolamine, an unidentified aminophospholipid, phosphatidylinositol, an unidentified phospholipid, an unidentified phosphoglycolipid and an unidentified glycolipid.
30465644	0	31	theme	Nakamurella	0:10	arg1	sp					20:21	Nakamurella deserti sp	0:21	Nakamurella deserti sp.	0:22	Nakamurella deserti sp.
30465644	6	32	theme	meso-diaminopimelic	876:894	arg1	acid					927:930	the diagnostic diamino acid	904:930	the diagnostic diamino acid of the peptidoglycan	904:951	Chemotaxonomic analyses showed that the isolate possessed meso-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan, glucose, mannose and galactose as whole-cell sugars, and MK-8(H4) as the predominant menaquinone.
30465644	6	32	theme	meso-diaminopimelic	876:894	arg1	acid					896:899	meso-diaminopimelic acid	876:899	meso-diaminopimelic acid	876:899	Chemotaxonomic analyses showed that the isolate possessed meso-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan, glucose, mannose and galactose as whole-cell sugars, and MK-8(H4) as the predominant menaquinone.
30465644	6	32	theme	meso-diaminopimelic	876:894	arg1	menaquinone					1039:1049	the predominant menaquinone	1023:1049	the predominant menaquinone	1023:1049	Chemotaxonomic analyses showed that the isolate possessed meso-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan, glucose, mannose and galactose as whole-cell sugars, and MK-8(H4) as the predominant menaquinone.
30465644	4	33	theme	16S	613:615	arg1	similarity					636:645	the highest 16S rRNA gene sequence similarity	601:645	the highest 16S rRNA gene sequence similarity to Nakamurella silvestris S20-107T	601:680	Phylogenetic analysis based on 16S rRNA gene sequence suggested that strain 12Sc4-1T belonged to the genus Nakamurella and shared the highest 16S rRNA gene sequence similarity to Nakamurella silvestris S20-107T (96.94 %).
30465644	4	34	theme	gene	622:625	arg1	similarity					636:645	the highest 16S rRNA gene sequence similarity	601:645	the highest 16S rRNA gene sequence similarity to Nakamurella silvestris S20-107T	601:680	Phylogenetic analysis based on 16S rRNA gene sequence suggested that strain 12Sc4-1T belonged to the genus Nakamurella and shared the highest 16S rRNA gene sequence similarity to Nakamurella silvestris S20-107T (96.94 %).
30465644	6	35	theme	diagnostic	908:917	arg1	acid					927:930	the diagnostic diamino acid	904:930	the diagnostic diamino acid of the peptidoglycan	904:951	Chemotaxonomic analyses showed that the isolate possessed meso-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan, glucose, mannose and galactose as whole-cell sugars, and MK-8(H4) as the predominant menaquinone.
30465644	6	35	theme	diagnostic	908:917	arg1	acid					896:899	meso-diaminopimelic acid	876:899	meso-diaminopimelic acid	876:899	Chemotaxonomic analyses showed that the isolate possessed meso-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan, glucose, mannose and galactose as whole-cell sugars, and MK-8(H4) as the predominant menaquinone.
30465644	8	36	dep	C16 	1309:1312	arg1	 0					1334:1335	 0	1334:1335	 0	1334:1335	The major fatty acids were C16 : 0 and anteiso-C15 : 0.
30465644	8	36	dep	C16 	1309:1312	arg1	anteiso-C15 					1321:1332	anteiso-C15 	1321:1332	anteiso-C15 	1321:1332	The major fatty acids were C16 : 0 and anteiso-C15 : 0.
30465644	8	36	dep	C16 	1309:1312	arg1	 0					1314:1315	 0	1314:1315	 0	1314:1315	The major fatty acids were C16 : 0 and anteiso-C15 : 0.
30465644	1	37	theme	Taklamakan	81:90	arg1	desert					92:97	the Taklamakan desert	77:97	the Taklamakan desert	77:97	nov., isolated from rhizosphere soil of Reaumuria in the Taklamakan desert.
30465644	5	38	theme	%	722:722	arg1	similarities					747:758	<96.0 % 16S rRNA gene sequence similarities	716:758	<96.0 % 16S rRNA gene sequence similarities to all other recognized members of the genus Nakamurella	716:815	Strain 12Sc4-1T showed <96.0 % 16S rRNA gene sequence similarities to all other recognized members of the genus Nakamurella.
30465644	5	39	theme	<96.0 	716:721	arg1	%					722:722	%	722:722	%	722:722	Strain 12Sc4-1T showed <96.0 % 16S rRNA gene sequence similarities to all other recognized members of the genus Nakamurella.
30465644	6	40	theme	whole-cell	988:997	arg1	sugars					999:1004	whole-cell sugars	988:1004	whole-cell sugars	988:1004	Chemotaxonomic analyses showed that the isolate possessed meso-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan, glucose, mannose and galactose as whole-cell sugars, and MK-8(H4) as the predominant menaquinone.
30465644	5	41	theme	16S	724:726	arg1	similarities					747:758	<96.0 % 16S rRNA gene sequence similarities	716:758	<96.0 % 16S rRNA gene sequence similarities to all other recognized members of the genus Nakamurella	716:815	Strain 12Sc4-1T showed <96.0 % 16S rRNA gene sequence similarities to all other recognized members of the genus Nakamurella.
30465644	5	42	theme	rRNA	728:731	arg1	similarities					747:758	<96.0 % 16S rRNA gene sequence similarities	716:758	<96.0 % 16S rRNA gene sequence similarities to all other recognized members of the genus Nakamurella	716:815	Strain 12Sc4-1T showed <96.0 % 16S rRNA gene sequence similarities to all other recognized members of the genus Nakamurella.
30465644	9	43	theme	draft	1373:1377	arg1	sequence					1386:1393	draft genome sequence	1373:1393	draft genome sequence	1373:1393	The DNA G+C content was 72.1 mol% (draft genome sequence).
30465644	9	43	theme	draft	1373:1377	arg1	%					1370:1370	72.1 mol%	1362:1370	72.1 mol% (draft genome sequence)	1362:1394	The DNA G+C content was 72.1 mol% (draft genome sequence).
30465644	4	44	theme	strain	540:545	arg1	12Sc4-1T					547:554	strain 12Sc4-1T	540:554	strain 12Sc4-1T	540:554	Phylogenetic analysis based on 16S rRNA gene sequence suggested that strain 12Sc4-1T belonged to the genus Nakamurella and shared the highest 16S rRNA gene sequence similarity to Nakamurella silvestris S20-107T (96.94 %).
30465644	9	45	theme	72.1 mol	1362:1369	arg1	sequence					1386:1393	draft genome sequence	1373:1393	draft genome sequence	1373:1393	The DNA G+C content was 72.1 mol% (draft genome sequence).
30465644	9	45	theme	72.1 mol	1362:1369	arg1	G+C content					1346:1356	The DNA G+C content	1338:1356	The DNA G+C content	1338:1356	The DNA G+C content was 72.1 mol% (draft genome sequence).
30465644	9	45	theme	72.1 mol	1362:1369	arg1	%					1370:1370	72.1 mol%	1362:1370	72.1 mol% (draft genome sequence)	1362:1394	The DNA G+C content was 72.1 mol% (draft genome sequence).
30465644	5	46	theme	gene	733:736	arg1	similarities					747:758	<96.0 % 16S rRNA gene sequence similarities	716:758	<96.0 % 16S rRNA gene sequence similarities to all other recognized members of the genus Nakamurella	716:815	Strain 12Sc4-1T showed <96.0 % 16S rRNA gene sequence similarities to all other recognized members of the genus Nakamurella.
30465644	10	47	dep	analyses	1457:1464	arg1	basis					1404:1408	basis	1404:1408	basis	1404:1408	On the basis of phylogenetic, phenotypic and chemotaxonomic analyses, strain 12Sc4-1T represents a novel species of the genus Nakamurella, for which the name Nakamurelladeserti sp.
30465644	10	47	dep	analyses	1457:1464	arg1	the					1400:1402	the	1400:1402	the	1400:1402	On the basis of phylogenetic, phenotypic and chemotaxonomic analyses, strain 12Sc4-1T represents a novel species of the genus Nakamurella, for which the name Nakamurelladeserti sp.
30465644	6	48	theme	peptidoglycan	939:951	arg1	H4					1016:1017	H4	1016:1017	H4	1016:1017	Chemotaxonomic analyses showed that the isolate possessed meso-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan, glucose, mannose and galactose as whole-cell sugars, and MK-8(H4) as the predominant menaquinone.
30465644	6	48	theme	peptidoglycan	939:951	arg1	glucose					954:960	glucose	954:960	glucose	954:960	Chemotaxonomic analyses showed that the isolate possessed meso-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan, glucose, mannose and galactose as whole-cell sugars, and MK-8(H4) as the predominant menaquinone.
30465644	6	48	theme	peptidoglycan	939:951	arg1	acid					927:930	the diagnostic diamino acid	904:930	the diagnostic diamino acid of the peptidoglycan	904:951	Chemotaxonomic analyses showed that the isolate possessed meso-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan, glucose, mannose and galactose as whole-cell sugars, and MK-8(H4) as the predominant menaquinone.
30465644	6	48	theme	peptidoglycan	939:951	arg1	mannose					963:969	mannose	963:969	mannose	963:969	Chemotaxonomic analyses showed that the isolate possessed meso-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan, glucose, mannose and galactose as whole-cell sugars, and MK-8(H4) as the predominant menaquinone.
30465644	6	48	theme	peptidoglycan	939:951	arg1	acid					896:899	meso-diaminopimelic acid	876:899	meso-diaminopimelic acid	876:899	Chemotaxonomic analyses showed that the isolate possessed meso-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan, glucose, mannose and galactose as whole-cell sugars, and MK-8(H4) as the predominant menaquinone.
30465644	6	48	theme	peptidoglycan	939:951	arg1	galactose					975:983	galactose	975:983	galactose	975:983	Chemotaxonomic analyses showed that the isolate possessed meso-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan, glucose, mannose and galactose as whole-cell sugars, and MK-8(H4) as the predominant menaquinone.
30465644	6	48	theme	peptidoglycan	939:951	arg1	MK-8					1011:1014	MK-8	1011:1014	MK-8(H4)	1011:1018	Chemotaxonomic analyses showed that the isolate possessed meso-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan, glucose, mannose and galactose as whole-cell sugars, and MK-8(H4) as the predominant menaquinone.
30465644	12	49	theme	type	1600:1603	arg1	12Sc4-1T					1615:1622	12Sc4-1T	1615:1622	12Sc4-1T (=KCTC 49114T=CGMCC 1.16555T)	1615:1652	The type strain is 12Sc4-1T (=KCTC 49114T=CGMCC 1.16555T).
30465644	12	49	theme	type	1600:1603	arg1	strain					1605:1610	The type strain	1596:1610	The type strain	1596:1610	The type strain is 12Sc4-1T (=KCTC 49114T=CGMCC 1.16555T).
30465644	3	50	dep	optimum	398:404	arg1	pH					407:408	pH 7.0	407:412	pH 7.0	407:412	Strain 12Sc4-1T grew at 8‒35 °C (optimum, 28‒30 °C), pH 6.0‒9.0 (optimum, pH 7.0) and in the presence of 0‒3 % (w/v) NaCl (optimum, 0 %).
30465644	4	51	theme	highest	605:611	arg1	similarity					636:645	the highest 16S rRNA gene sequence similarity	601:645	the highest 16S rRNA gene sequence similarity to Nakamurella silvestris S20-107T	601:680	Phylogenetic analysis based on 16S rRNA gene sequence suggested that strain 12Sc4-1T belonged to the genus Nakamurella and shared the highest 16S rRNA gene sequence similarity to Nakamurella silvestris S20-107T (96.94 %).
30465644	10	52	theme	Nakamurella	1523:1533	arg1	species					1502:1508	a novel species	1494:1508	a novel species	1494:1508	On the basis of phylogenetic, phenotypic and chemotaxonomic analyses, strain 12Sc4-1T represents a novel species of the genus Nakamurella, for which the name Nakamurelladeserti sp.
30465644	10	53	theme	phenotypic	1427:1436	arg1	analyses					1457:1464	phylogenetic, phenotypic and chemotaxonomic analyses	1413:1464	phylogenetic, phenotypic and chemotaxonomic analyses	1413:1464	On the basis of phylogenetic, phenotypic and chemotaxonomic analyses, strain 12Sc4-1T represents a novel species of the genus Nakamurella, for which the name Nakamurelladeserti sp.
30465644	5	54	theme	genus	799:803	arg1	Nakamurella					805:815	the genus Nakamurella	795:815	the genus Nakamurella	795:815	Strain 12Sc4-1T showed <96.0 % 16S rRNA gene sequence similarities to all other recognized members of the genus Nakamurella.
30465644	2	55	dep	Gram-stain-positive	102:120	arg1	coccus-shaped					163:175	coccus-shaped	163:175	coccus-shaped	163:175	A Gram-stain-positive, aerobic, non-motile, non-spore-forming, coccus-shaped actinobacterium, designated strain 12Sc4-1T, was isolated from a soil sample collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, China.
30465644	2	55	dep	Gram-stain-positive	102:120	arg1	non-spore-forming					144:160	non-spore-forming	144:160	non-spore-forming	144:160	A Gram-stain-positive, aerobic, non-motile, non-spore-forming, coccus-shaped actinobacterium, designated strain 12Sc4-1T, was isolated from a soil sample collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, China.
30465644	2	55	dep	Gram-stain-positive	102:120	arg1	non-motile					132:141	non-motile	132:141	non-motile	132:141	A Gram-stain-positive, aerobic, non-motile, non-spore-forming, coccus-shaped actinobacterium, designated strain 12Sc4-1T, was isolated from a soil sample collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, China.
30465644	2	55	dep	Gram-stain-positive	102:120	arg1	aerobic					123:129	aerobic	123:129	aerobic	123:129	A Gram-stain-positive, aerobic, non-motile, non-spore-forming, coccus-shaped actinobacterium, designated strain 12Sc4-1T, was isolated from a soil sample collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, China.
30465644	2	56	theme	strain	205:210	arg1	12Sc4-1T					212:219	strain 12Sc4-1T	205:219	strain 12Sc4-1T	205:219	A Gram-stain-positive, aerobic, non-motile, non-spore-forming, coccus-shaped actinobacterium, designated strain 12Sc4-1T, was isolated from a soil sample collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, China.
30465644	4	57	theme	genus	572:576	arg1	Nakamurella					578:588	the genus Nakamurella	568:588	the genus Nakamurella	568:588	Phylogenetic analysis based on 16S rRNA gene sequence suggested that strain 12Sc4-1T belonged to the genus Nakamurella and shared the highest 16S rRNA gene sequence similarity to Nakamurella silvestris S20-107T (96.94 %).
30465644	4	58	theme	rRNA	617:620	arg1	similarity					636:645	the highest 16S rRNA gene sequence similarity	601:645	the highest 16S rRNA gene sequence similarity to Nakamurella silvestris S20-107T	601:680	Phylogenetic analysis based on 16S rRNA gene sequence suggested that strain 12Sc4-1T belonged to the genus Nakamurella and shared the highest 16S rRNA gene sequence similarity to Nakamurella silvestris S20-107T (96.94 %).
30465644	4	59	theme	16S	502:504	arg1	rRNA					506:509	16S rRNA	502:509	16S rRNA gene sequence	502:523	Phylogenetic analysis based on 16S rRNA gene sequence suggested that strain 12Sc4-1T belonged to the genus Nakamurella and shared the highest 16S rRNA gene sequence similarity to Nakamurella silvestris S20-107T (96.94 %).
30465644	3	60	theme	NaCl	450:453	arg1	presence					426:433	the presence	422:433	the presence of 0‒3 % (w/v) NaCl (optimum, 0 %)	422:468	Strain 12Sc4-1T grew at 8‒35 °C (optimum, 28‒30 °C), pH 6.0‒9.0 (optimum, pH 7.0) and in the presence of 0‒3 % (w/v) NaCl (optimum, 0 %).
30465644	4	61	dep	shared	594:599	arg1	%					689:689	96.94 %	683:689	96.94 %	683:689	Phylogenetic analysis based on 16S rRNA gene sequence suggested that strain 12Sc4-1T belonged to the genus Nakamurella and shared the highest 16S rRNA gene sequence similarity to Nakamurella silvestris S20-107T (96.94 %).
30465644	2	62	dep	Uygur	301:305	arg1	Region					318:323	Autonomous Region	307:323	Autonomous Region	307:323	A Gram-stain-positive, aerobic, non-motile, non-spore-forming, coccus-shaped actinobacterium, designated strain 12Sc4-1T, was isolated from a soil sample collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, China.
30465644	12	63	theme	=KCTC	1625:1629	arg1	12Sc4-1T					1615:1622	12Sc4-1T	1615:1622	12Sc4-1T (=KCTC 49114T=CGMCC 1.16555T)	1615:1652	The type strain is 12Sc4-1T (=KCTC 49114T=CGMCC 1.16555T).
30465644	12	63	theme	=KCTC	1625:1629	arg1	1.16555T					1644:1651	=KCTC 49114T=CGMCC 1.16555T	1625:1651	=KCTC 49114T=CGMCC 1.16555T	1625:1651	The type strain is 12Sc4-1T (=KCTC 49114T=CGMCC 1.16555T).
30465644	5	64	theme	Nakamurella	805:815	arg1	members					784:790	all other recognized members	763:790	all other recognized members of the genus Nakamurella	763:815	Strain 12Sc4-1T showed <96.0 % 16S rRNA gene sequence similarities to all other recognized members of the genus Nakamurella.
30465644	10	65	theme	strain	1467:1472	arg1	12Sc4-1T					1474:1481	strain 12Sc4-1T	1467:1481	strain 12Sc4-1T	1467:1481	On the basis of phylogenetic, phenotypic and chemotaxonomic analyses, strain 12Sc4-1T represents a novel species of the genus Nakamurella, for which the name Nakamurelladeserti sp.
30465644	5	66	theme	recognized	773:782	arg1	members					784:790	all other recognized members	763:790	all other recognized members of the genus Nakamurella	763:815	Strain 12Sc4-1T showed <96.0 % 16S rRNA gene sequence similarities to all other recognized members of the genus Nakamurella.
30465644	2	67	theme	soil	242:245	arg1	sample					247:252	a soil sample	240:252	a soil sample collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, China	240:330	A Gram-stain-positive, aerobic, non-motile, non-spore-forming, coccus-shaped actinobacterium, designated strain 12Sc4-1T, was isolated from a soil sample collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, China.
30465644	9	68	theme	genome	1379:1384	arg1	sequence					1386:1393	draft genome sequence	1373:1393	draft genome sequence	1373:1393	The DNA G+C content was 72.1 mol% (draft genome sequence).
30465644	9	68	theme	genome	1379:1384	arg1	%					1370:1370	72.1 mol%	1362:1370	72.1 mol% (draft genome sequence)	1362:1394	The DNA G+C content was 72.1 mol% (draft genome sequence).
30465644	5	69	theme	other	767:771	arg1	members					784:790	all other recognized members	763:790	all other recognized members of the genus Nakamurella	763:815	Strain 12Sc4-1T showed <96.0 % 16S rRNA gene sequence similarities to all other recognized members of the genus Nakamurella.
30465644	4	70	theme	gene	511:514	arg1	sequence					516:523	16S rRNA gene sequence	502:523	16S rRNA gene sequence	502:523	Phylogenetic analysis based on 16S rRNA gene sequence suggested that strain 12Sc4-1T belonged to the genus Nakamurella and shared the highest 16S rRNA gene sequence similarity to Nakamurella silvestris S20-107T (96.94 %).
30465644	6	71	theme	diamino	919:925	arg1	acid					927:930	the diagnostic diamino acid	904:930	the diagnostic diamino acid of the peptidoglycan	904:951	Chemotaxonomic analyses showed that the isolate possessed meso-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan, glucose, mannose and galactose as whole-cell sugars, and MK-8(H4) as the predominant menaquinone.
30465644	6	71	theme	diamino	919:925	arg1	acid					896:899	meso-diaminopimelic acid	876:899	meso-diaminopimelic acid	876:899	Chemotaxonomic analyses showed that the isolate possessed meso-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan, glucose, mannose and galactose as whole-cell sugars, and MK-8(H4) as the predominant menaquinone.
30465644	7	72	theme	unidentified	1189:1200	arg1	phospholipid					1202:1213	an unidentified phospholipid	1186:1213	an unidentified phospholipid	1186:1213	The polar lipids comprised diphosphatidylglycerol, phosphatidylethanolamine, an unidentified aminophospholipid, phosphatidylinositol, an unidentified phospholipid, an unidentified phosphoglycolipid and an unidentified glycolipid.
30465644	3	73	theme	w/v	445:447	arg1	optimum					456:462	optimum	456:462	optimum	456:462	Strain 12Sc4-1T grew at 8‒35 °C (optimum, 28‒30 °C), pH 6.0‒9.0 (optimum, pH 7.0) and in the presence of 0‒3 % (w/v) NaCl (optimum, 0 %).
30465644	3	73	theme	w/v	445:447	arg1	NaCl					450:453	0‒3 % (w/v) NaCl	438:453	0‒3 % (w/v) NaCl (optimum, 0 %)	438:468	Strain 12Sc4-1T grew at 8‒35 °C (optimum, 28‒30 °C), pH 6.0‒9.0 (optimum, pH 7.0) and in the presence of 0‒3 % (w/v) NaCl (optimum, 0 %).
30465644	4	74	theme	rRNA	506:509	arg1	sequence					516:523	16S rRNA gene sequence	502:523	16S rRNA gene sequence	502:523	Phylogenetic analysis based on 16S rRNA gene sequence suggested that strain 12Sc4-1T belonged to the genus Nakamurella and shared the highest 16S rRNA gene sequence similarity to Nakamurella silvestris S20-107T (96.94 %).
30156528	4	0	theme	strain	420:425	arg1	SYF10-1aT					427:435	strain SYF10-1aT	420:435	strain SYF10-1aT	420:435	Phylogenetic analysis using 16S rRNA gene sequences revealed that strain SYF10-1aT belonged to the genus Lactobacillus and showed the highest sequence similarity of 98.7 % to Lactobacillus crustorum LMG 23699T.
30156528	13	1	theme	=KACC	1590:1594	arg1	112011T					1608:1614	=KACC 18726T=NBRC 112011T	1590:1614	=KACC 18726T=NBRC 112011T	1590:1614	The type strain is SYF10-1aT (=KACC 18726T=NBRC 112011T).
30156528	13	1	theme	=KACC	1590:1594	arg1	SYF10-1aT					1579:1587	SYF10-1aT	1579:1587	SYF10-1aT (=KACC 18726T=NBRC 112011T)	1579:1615	The type strain is SYF10-1aT (=KACC 18726T=NBRC 112011T).
30156528	11	2	theme	Lactobacillus	1517:1529	arg1	sp					1538:1539	the name Lactobacillus nuruki sp	1508:1539	the name Lactobacillus nuruki sp	1508:1539	On the basis of this taxonomic study, strain SYF10-1aT represents a novel species within the genus Lactobacillus, for which the name Lactobacillus nuruki sp.
30156528	6	3	theme	crustorum	921:929	arg1	SYF10-1aT					871:879	strain SYF10-1aT	864:879	strain SYF10-1aT	864:879	The average nucleotide identity value and digital DNA-DNA hybridization value between strain SYF10-1aT and the most closely related species,L. crustorum LMG 23699T, were 80.5 and 33.3 %, respectively.
30156528	6	3	theme	crustorum	921:929	arg1	23699T					935:940	L. crustorum LMG 23699T	918:940	L. crustorum LMG 23699T	918:940	The average nucleotide identity value and digital DNA-DNA hybridization value between strain SYF10-1aT and the most closely related species,L. crustorum LMG 23699T, were 80.5 and 33.3 %, respectively.
30156528	6	4	theme	hybridization	836:848	arg1	value					850:854	digital DNA-DNA hybridization value	820:854	digital DNA-DNA hybridization value	820:854	The average nucleotide identity value and digital DNA-DNA hybridization value between strain SYF10-1aT and the most closely related species,L. crustorum LMG 23699T, were 80.5 and 33.3 %, respectively.
30156528	6	5	theme	DNA-DNA	828:834	arg1	hybridization					836:848	digital DNA-DNA hybridization	820:848	digital DNA-DNA hybridization value	820:854	The average nucleotide identity value and digital DNA-DNA hybridization value between strain SYF10-1aT and the most closely related species,L. crustorum LMG 23699T, were 80.5 and 33.3 %, respectively.
30156528	13	6	theme	type	1564:1567	arg1	SYF10-1aT					1579:1587	SYF10-1aT	1579:1587	SYF10-1aT (=KACC 18726T=NBRC 112011T)	1579:1615	The type strain is SYF10-1aT (=KACC 18726T=NBRC 112011T).
30156528	13	6	theme	type	1564:1567	arg1	strain					1569:1574	The type strain	1560:1574	The type strain	1560:1574	The type strain is SYF10-1aT (=KACC 18726T=NBRC 112011T).
30156528	7	7	dep	 1ω7c	1047:1051	arg1	including					1075:1083	including	1075:1083	including iso-C15 : 0 2-OH and/or C16 	1075:1112	The predominant cellular fatty acids were C16 : 0, C18 : 1ω9c, C18 : 1ω7c and summed feature 3 (including iso-C15 : 0 2-OH and/or C16 : 1ω7c).
30156528	7	7	dep	 1ω7c	1047:1051	arg1	3					1072:1072	3	1072:1072	3	1072:1072	The predominant cellular fatty acids were C16 : 0, C18 : 1ω9c, C18 : 1ω7c and summed feature 3 (including iso-C15 : 0 2-OH and/or C16 : 1ω7c).
30156528	6	8	theme	L.	918:919	arg1	SYF10-1aT					871:879	strain SYF10-1aT	864:879	strain SYF10-1aT	864:879	The average nucleotide identity value and digital DNA-DNA hybridization value between strain SYF10-1aT and the most closely related species,L. crustorum LMG 23699T, were 80.5 and 33.3 %, respectively.
30156528	6	8	theme	L.	918:919	arg1	23699T					935:940	L. crustorum LMG 23699T	918:940	L. crustorum LMG 23699T	918:940	The average nucleotide identity value and digital DNA-DNA hybridization value between strain SYF10-1aT and the most closely related species,L. crustorum LMG 23699T, were 80.5 and 33.3 %, respectively.
30156528	2	9	theme	rod-shaped	84:93	arg1	bacterium					158:166	A rod-shaped, Gram-stain-positive, non-flagellated, facultatively anaerobic bacterium	82:166	A rod-shaped, Gram-stain-positive, non-flagellated, facultatively anaerobic bacterium	82:166	A rod-shaped, Gram-stain-positive, non-flagellated, facultatively anaerobic bacterium, designated SYF10-1aT, was isolated from Nuruk, a Korean traditional fermentation starter.
30156528	5	10	theme	housekeeping	585:596	arg1	rpoA					614:617	rpoA	614:617	rpoA	614:617	A comparison of two housekeeping genes, pheS and rpoA, supported the suggestion that strain SYF10-1aT fell within the radius of the genus Lactobacillus, but was clearly separated from its closest related species.
30156528	5	10	theme	housekeeping	585:596	arg1	pheS					605:608	pheS	605:608	pheS	605:608	A comparison of two housekeeping genes, pheS and rpoA, supported the suggestion that strain SYF10-1aT fell within the radius of the genus Lactobacillus, but was clearly separated from its closest related species.
30156528	5	10	theme	housekeeping	585:596	arg1	genes					598:602	two housekeeping genes	581:602	two housekeeping genes	581:602	A comparison of two housekeeping genes, pheS and rpoA, supported the suggestion that strain SYF10-1aT fell within the radius of the genus Lactobacillus, but was clearly separated from its closest related species.
30156528	7	11	dep	C16 	1021:1024	arg1	feature					1064:1070	summed feature	1057:1070	summed feature	1057:1070	The predominant cellular fatty acids were C16 : 0, C18 : 1ω9c, C18 : 1ω7c and summed feature 3 (including iso-C15 : 0 2-OH and/or C16 : 1ω7c).
30156528	7	11	dep	C16 	1021:1024	arg1	C18 					1042:1045	C18 	1042:1045	C18 	1042:1045	The predominant cellular fatty acids were C16 : 0, C18 : 1ω9c, C18 : 1ω7c and summed feature 3 (including iso-C15 : 0 2-OH and/or C16 : 1ω7c).
30156528	7	11	dep	C16 	1021:1024	arg1	C18 					1030:1033	C18 	1030:1033	C18 	1030:1033	The predominant cellular fatty acids were C16 : 0, C18 : 1ω9c, C18 : 1ω7c and summed feature 3 (including iso-C15 : 0 2-OH and/or C16 : 1ω7c).
30156528	7	11	dep	C16 	1021:1024	arg1	 1ω7c					1047:1051	 1ω7c	1047:1051	 1ω7c	1047:1051	The predominant cellular fatty acids were C16 : 0, C18 : 1ω9c, C18 : 1ω7c and summed feature 3 (including iso-C15 : 0 2-OH and/or C16 : 1ω7c).
30156528	1	12	theme	Nuruk	44:48	arg1	starter					73:79	Nuruk, a Korean fermentation starter	44:79	starter	73:79	nov., isolated from Nuruk, a Korean fermentation starter.
30156528	4	13	theme	%	524:524	arg1	similarity					505:514	the highest sequence similarity	484:514	the highest sequence similarity of 98.7 % to Lactobacillus crustorum LMG 23699T	484:562	Phylogenetic analysis using 16S rRNA gene sequences revealed that strain SYF10-1aT belonged to the genus Lactobacillus and showed the highest sequence similarity of 98.7 % to Lactobacillus crustorum LMG 23699T.
30156528	5	14	theme	strain	650:655	arg1	SYF10-1aT					657:665	strain SYF10-1aT	650:665	strain SYF10-1aT	650:665	A comparison of two housekeeping genes, pheS and rpoA, supported the suggestion that strain SYF10-1aT fell within the radius of the genus Lactobacillus, but was clearly separated from its closest related species.
30156528	11	15	theme	nuruki	1531:1536	arg1	sp					1538:1539	the name Lactobacillus nuruki sp	1508:1539	the name Lactobacillus nuruki sp	1508:1539	On the basis of this taxonomic study, strain SYF10-1aT represents a novel species within the genus Lactobacillus, for which the name Lactobacillus nuruki sp.
30156528	5	16	theme	genes	598:602	arg1	comparison					567:576	A comparison	565:576	A comparison of two housekeeping genes, pheS and rpoA,	565:618	A comparison of two housekeeping genes, pheS and rpoA, supported the suggestion that strain SYF10-1aT fell within the radius of the genus Lactobacillus, but was clearly separated from its closest related species.
30156528	8	17	theme	unidentified	1190:1201	arg1	aminolipids					1203:1213	two unidentified aminolipids	1186:1213	two unidentified aminolipids	1186:1213	Polar lipids were diphosphatidylglycerol, phosphatidylglycerol, two unidentified aminolipids and two unidentified glycolipids.
30156528	6	18	theme	LMG	931:933	arg1	SYF10-1aT					871:879	strain SYF10-1aT	864:879	strain SYF10-1aT	864:879	The average nucleotide identity value and digital DNA-DNA hybridization value between strain SYF10-1aT and the most closely related species,L. crustorum LMG 23699T, were 80.5 and 33.3 %, respectively.
30156528	6	18	theme	LMG	931:933	arg1	23699T					935:940	L. crustorum LMG 23699T	918:940	L. crustorum LMG 23699T	918:940	The average nucleotide identity value and digital DNA-DNA hybridization value between strain SYF10-1aT and the most closely related species,L. crustorum LMG 23699T, were 80.5 and 33.3 %, respectively.
30156528	4	19	theme	gene	391:394	arg1	sequences					396:404	16S rRNA gene sequences	382:404	16S rRNA gene sequences	382:404	Phylogenetic analysis using 16S rRNA gene sequences revealed that strain SYF10-1aT belonged to the genus Lactobacillus and showed the highest sequence similarity of 98.7 % to Lactobacillus crustorum LMG 23699T.
30156528	7	20	theme	fatty	1004:1008	arg1	C16 					1021:1024	C16 	1021:1024	C16 	1021:1024	The predominant cellular fatty acids were C16 : 0, C18 : 1ω9c, C18 : 1ω7c and summed feature 3 (including iso-C15 : 0 2-OH and/or C16 : 1ω7c).
30156528	7	20	theme	fatty	1004:1008	arg1	acids					1010:1014	The predominant cellular fatty acids	979:1014	The predominant cellular fatty acids	979:1014	The predominant cellular fatty acids were C16 : 0, C18 : 1ω9c, C18 : 1ω7c and summed feature 3 (including iso-C15 : 0 2-OH and/or C16 : 1ω7c).
30156528	2	21	attach	isolated	195:202	arg2	bacterium					158:166	A rod-shaped, Gram-stain-positive, non-flagellated, facultatively anaerobic bacterium	82:166	A rod-shaped, Gram-stain-positive, non-flagellated, facultatively anaerobic bacterium	82:166	A rod-shaped, Gram-stain-positive, non-flagellated, facultatively anaerobic bacterium, designated SYF10-1aT, was isolated from Nuruk, a Korean traditional fermentation starter.
30156528	2	21	attach	isolated	195:202	arg1	Nuruk					209:213	Nuruk	209:213	Nuruk	209:213	A rod-shaped, Gram-stain-positive, non-flagellated, facultatively anaerobic bacterium, designated SYF10-1aT, was isolated from Nuruk, a Korean traditional fermentation starter.
30156528	2	21	attach	isolated	195:202	arg1	starter					250:256	a Korean traditional fermentation starter	216:256	a Korean traditional fermentation starter	216:256	A rod-shaped, Gram-stain-positive, non-flagellated, facultatively anaerobic bacterium, designated SYF10-1aT, was isolated from Nuruk, a Korean traditional fermentation starter.
30156528	7	22	theme	summed	1057:1062	arg1	feature					1064:1070	summed feature	1057:1070	summed feature	1057:1070	The predominant cellular fatty acids were C16 : 0, C18 : 1ω9c, C18 : 1ω7c and summed feature 3 (including iso-C15 : 0 2-OH and/or C16 : 1ω7c).
30156528	11	23	theme	name	1512:1515	arg1	sp					1538:1539	the name Lactobacillus nuruki sp	1508:1539	the name Lactobacillus nuruki sp	1508:1539	On the basis of this taxonomic study, strain SYF10-1aT represents a novel species within the genus Lactobacillus, for which the name Lactobacillus nuruki sp.
30156528	3	24	dep	%	340:340	arg1	w/v					343:345	w/v	343:345	w/v	343:345	It grew at 4-40 °C (optimum 37 °C), at pH 3.0-9.0 (optimum, pH 7.0) and with 0-5 % (w/v) NaCl.
30156528	10	25	theme	G+C	1357:1359	arg1	content					1361:1367	The DNA G+C content	1349:1367	The DNA G+C content	1349:1367	The DNA G+C content was 34.2 mol%.
30156528	10	25	theme	G+C	1357:1359	arg1	%					1381:1381	34.2 mol%	1373:1381	34.2 mol%	1373:1381	The DNA G+C content was 34.2 mol%.
30156528	1	26	theme	Korean	53:58	arg1	starter					73:79	Nuruk, a Korean fermentation starter	44:79	starter	73:79	nov., isolated from Nuruk, a Korean fermentation starter.
30156528	9	27	theme	interpeptide	1305:1316	arg1	bridge					1318:1323	an interpeptide bridge	1302:1323	an interpeptide bridge comprising l-Lys-d-Asp	1302:1346	The cell-wall peptidoglycan was of the A4α type with an interpeptide bridge comprising l-Lys-d-Asp.
30156528	0	28	theme	nuruki	13:18	arg1	sp					20:21	Lactobacilus nuruki sp	0:21	Lactobacilus nuruki sp.	0:22	Lactobacilus nuruki sp.
30156528	6	29	theme	nucleotide	790:799	arg1	%					962:962	80.5 and 33.3 %	948:962	%	962:962	The average nucleotide identity value and digital DNA-DNA hybridization value between strain SYF10-1aT and the most closely related species,L. crustorum LMG 23699T, were 80.5 and 33.3 %, respectively.
30156528	6	29	theme	nucleotide	790:799	arg1	identity					801:808	The average nucleotide identity value and digital DNA-DNA hybridization value	778:854	The average nucleotide identity value and digital DNA-DNA hybridization value between strain SYF10-1aT and the most closely related species,L. crustorum LMG 23699T,	778:941	The average nucleotide identity value and digital DNA-DNA hybridization value between strain SYF10-1aT and the most closely related species,L. crustorum LMG 23699T, were 80.5 and 33.3 %, respectively.
30156528	7	30	theme	predominant	983:993	arg1	C16 					1021:1024	C16 	1021:1024	C16 	1021:1024	The predominant cellular fatty acids were C16 : 0, C18 : 1ω9c, C18 : 1ω7c and summed feature 3 (including iso-C15 : 0 2-OH and/or C16 : 1ω7c).
30156528	7	30	theme	predominant	983:993	arg1	acids					1010:1014	The predominant cellular fatty acids	979:1014	The predominant cellular fatty acids	979:1014	The predominant cellular fatty acids were C16 : 0, C18 : 1ω9c, C18 : 1ω7c and summed feature 3 (including iso-C15 : 0 2-OH and/or C16 : 1ω7c).
30156528	1	31	theme	fermentation	60:71	arg1	starter					73:79	Nuruk, a Korean fermentation starter	44:79	starter	73:79	nov., isolated from Nuruk, a Korean fermentation starter.
30156528	0	32	theme	Lactobacilus	0:11	arg1	sp					20:21	Lactobacilus nuruki sp	0:21	Lactobacilus nuruki sp.	0:22	Lactobacilus nuruki sp.
30156528	6	33	theme	related	902:908	arg1	species					910:916	the most closely related species	885:916	the most closely related species	885:916	The average nucleotide identity value and digital DNA-DNA hybridization value between strain SYF10-1aT and the most closely related species,L. crustorum LMG 23699T, were 80.5 and 33.3 %, respectively.
30156528	7	34	theme	cellular	995:1002	arg1	C16 					1021:1024	C16 	1021:1024	C16 	1021:1024	The predominant cellular fatty acids were C16 : 0, C18 : 1ω9c, C18 : 1ω7c and summed feature 3 (including iso-C15 : 0 2-OH and/or C16 : 1ω7c).
30156528	7	34	theme	cellular	995:1002	arg1	acids					1010:1014	The predominant cellular fatty acids	979:1014	The predominant cellular fatty acids	979:1014	The predominant cellular fatty acids were C16 : 0, C18 : 1ω9c, C18 : 1ω7c and summed feature 3 (including iso-C15 : 0 2-OH and/or C16 : 1ω7c).
30156528	8	35	theme	unidentified	1223:1234	arg1	glycolipids					1236:1246	two unidentified glycolipids	1219:1246	two unidentified glycolipids	1219:1246	Polar lipids were diphosphatidylglycerol, phosphatidylglycerol, two unidentified aminolipids and two unidentified glycolipids.
30156528	10	36	theme	34.2 mol	1373:1380	arg1	content					1361:1367	The DNA G+C content	1349:1367	The DNA G+C content	1349:1367	The DNA G+C content was 34.2 mol%.
30156528	10	36	theme	34.2 mol	1373:1380	arg1	%					1381:1381	34.2 mol%	1373:1381	34.2 mol%	1373:1381	The DNA G+C content was 34.2 mol%.
30156528	6	37	theme	digital	820:826	arg1	hybridization					836:848	digital DNA-DNA hybridization	820:848	digital DNA-DNA hybridization value	820:854	The average nucleotide identity value and digital DNA-DNA hybridization value between strain SYF10-1aT and the most closely related species,L. crustorum LMG 23699T, were 80.5 and 33.3 %, respectively.
30156528	4	38	theme	sequence	496:503	arg1	similarity					505:514	the highest sequence similarity	484:514	the highest sequence similarity of 98.7 % to Lactobacillus crustorum LMG 23699T	484:562	Phylogenetic analysis using 16S rRNA gene sequences revealed that strain SYF10-1aT belonged to the genus Lactobacillus and showed the highest sequence similarity of 98.7 % to Lactobacillus crustorum LMG 23699T.
30156528	7	39	dep	including	1075:1083	arg1	 1ω7c					1114:1118	 1ω7c	1114:1118	 1ω7c	1114:1118	The predominant cellular fatty acids were C16 : 0, C18 : 1ω9c, C18 : 1ω7c and summed feature 3 (including iso-C15 : 0 2-OH and/or C16 : 1ω7c).
30156528	6	40	theme	average	782:788	arg1	%					962:962	80.5 and 33.3 %	948:962	%	962:962	The average nucleotide identity value and digital DNA-DNA hybridization value between strain SYF10-1aT and the most closely related species,L. crustorum LMG 23699T, were 80.5 and 33.3 %, respectively.
30156528	6	40	theme	average	782:788	arg1	identity					801:808	The average nucleotide identity value and digital DNA-DNA hybridization value	778:854	The average nucleotide identity value and digital DNA-DNA hybridization value between strain SYF10-1aT and the most closely related species,L. crustorum LMG 23699T,	778:941	The average nucleotide identity value and digital DNA-DNA hybridization value between strain SYF10-1aT and the most closely related species,L. crustorum LMG 23699T, were 80.5 and 33.3 %, respectively.
30156528	6	41	theme	33.3 	957:961	arg1	%					962:962	80.5 and 33.3 %	948:962	%	962:962	The average nucleotide identity value and digital DNA-DNA hybridization value between strain SYF10-1aT and the most closely related species,L. crustorum LMG 23699T, were 80.5 and 33.3 %, respectively.
30156528	6	41	theme	33.3 	957:961	arg1	identity					801:808	The average nucleotide identity value and digital DNA-DNA hybridization value	778:854	The average nucleotide identity value and digital DNA-DNA hybridization value between strain SYF10-1aT and the most closely related species,L. crustorum LMG 23699T,	778:941	The average nucleotide identity value and digital DNA-DNA hybridization value between strain SYF10-1aT and the most closely related species,L. crustorum LMG 23699T, were 80.5 and 33.3 %, respectively.
30156528	4	42	theme	highest	488:494	arg1	similarity					505:514	the highest sequence similarity	484:514	the highest sequence similarity of 98.7 % to Lactobacillus crustorum LMG 23699T	484:562	Phylogenetic analysis using 16S rRNA gene sequences revealed that strain SYF10-1aT belonged to the genus Lactobacillus and showed the highest sequence similarity of 98.7 % to Lactobacillus crustorum LMG 23699T.
30156528	11	43	theme	genus	1477:1481	arg1	Lactobacillus					1483:1495	the genus Lactobacillus	1473:1495	the genus Lactobacillus	1473:1495	On the basis of this taxonomic study, strain SYF10-1aT represents a novel species within the genus Lactobacillus, for which the name Lactobacillus nuruki sp.
30156528	2	44	theme	Korean	218:223	arg1	Nuruk					209:213	Nuruk	209:213	Nuruk	209:213	A rod-shaped, Gram-stain-positive, non-flagellated, facultatively anaerobic bacterium, designated SYF10-1aT, was isolated from Nuruk, a Korean traditional fermentation starter.
30156528	2	44	theme	Korean	218:223	arg1	starter					250:256	a Korean traditional fermentation starter	216:256	a Korean traditional fermentation starter	216:256	A rod-shaped, Gram-stain-positive, non-flagellated, facultatively anaerobic bacterium, designated SYF10-1aT, was isolated from Nuruk, a Korean traditional fermentation starter.
30156528	13	45	theme	18726T=NBRC	1596:1606	arg1	112011T					1608:1614	=KACC 18726T=NBRC 112011T	1590:1614	=KACC 18726T=NBRC 112011T	1590:1614	The type strain is SYF10-1aT (=KACC 18726T=NBRC 112011T).
30156528	13	45	theme	18726T=NBRC	1596:1606	arg1	SYF10-1aT					1579:1587	SYF10-1aT	1579:1587	SYF10-1aT (=KACC 18726T=NBRC 112011T)	1579:1615	The type strain is SYF10-1aT (=KACC 18726T=NBRC 112011T).
30156528	5	46	theme	closest	753:759	arg1	species					769:775	its closest related species	749:775	its closest related species	749:775	A comparison of two housekeeping genes, pheS and rpoA, supported the suggestion that strain SYF10-1aT fell within the radius of the genus Lactobacillus, but was clearly separated from its closest related species.
30156528	5	47	theme	genus	697:701	arg1	Lactobacillus					703:715	the genus Lactobacillus	693:715	the genus Lactobacillus	693:715	A comparison of two housekeeping genes, pheS and rpoA, supported the suggestion that strain SYF10-1aT fell within the radius of the genus Lactobacillus, but was clearly separated from its closest related species.
30156528	4	48	theme	genus	453:457	arg1	Lactobacillus					459:471	the genus Lactobacillus	449:471	the genus Lactobacillus	449:471	Phylogenetic analysis using 16S rRNA gene sequences revealed that strain SYF10-1aT belonged to the genus Lactobacillus and showed the highest sequence similarity of 98.7 % to Lactobacillus crustorum LMG 23699T.
30156528	9	49	theme	A4α	1288:1290	arg1	type					1292:1295	the A4α type	1284:1295	the A4α type	1284:1295	The cell-wall peptidoglycan was of the A4α type with an interpeptide bridge comprising l-Lys-d-Asp.
30156528	4	50	theme	rRNA	386:389	arg1	sequences					396:404	16S rRNA gene sequences	382:404	16S rRNA gene sequences	382:404	Phylogenetic analysis using 16S rRNA gene sequences revealed that strain SYF10-1aT belonged to the genus Lactobacillus and showed the highest sequence similarity of 98.7 % to Lactobacillus crustorum LMG 23699T.
30156528	9	51	with	type	1292:1295	arg1	bridge					1318:1323	an interpeptide bridge	1302:1323	an interpeptide bridge comprising l-Lys-d-Asp	1302:1346	The cell-wall peptidoglycan was of the A4α type with an interpeptide bridge comprising l-Lys-d-Asp.
30156528	5	52	theme	related	761:767	arg1	species					769:775	its closest related species	749:775	its closest related species	749:775	A comparison of two housekeeping genes, pheS and rpoA, supported the suggestion that strain SYF10-1aT fell within the radius of the genus Lactobacillus, but was clearly separated from its closest related species.
30156528	3	53	theme	0-5 	336:339	arg1	%					340:340	%	340:340	%	340:340	It grew at 4-40 °C (optimum 37 °C), at pH 3.0-9.0 (optimum, pH 7.0) and with 0-5 % (w/v) NaCl.
30156528	2	54	dep	rod-shaped	84:93	arg1	Gram-stain-positive					96:114	Gram-stain-positive	96:114	Gram-stain-positive	96:114	A rod-shaped, Gram-stain-positive, non-flagellated, facultatively anaerobic bacterium, designated SYF10-1aT, was isolated from Nuruk, a Korean traditional fermentation starter.
30156528	2	54	dep	rod-shaped	84:93	arg1	non-flagellated					117:131	non-flagellated	117:131	non-flagellated	117:131	A rod-shaped, Gram-stain-positive, non-flagellated, facultatively anaerobic bacterium, designated SYF10-1aT, was isolated from Nuruk, a Korean traditional fermentation starter.
30156528	2	54	dep	rod-shaped	84:93	arg1	anaerobic					148:156	anaerobic	148:156	anaerobic	148:156	A rod-shaped, Gram-stain-positive, non-flagellated, facultatively anaerobic bacterium, designated SYF10-1aT, was isolated from Nuruk, a Korean traditional fermentation starter.
30156528	4	55	theme	16S	382:384	arg1	sequences					396:404	16S rRNA gene sequences	382:404	16S rRNA gene sequences	382:404	Phylogenetic analysis using 16S rRNA gene sequences revealed that strain SYF10-1aT belonged to the genus Lactobacillus and showed the highest sequence similarity of 98.7 % to Lactobacillus crustorum LMG 23699T.
30156528	3	56	theme	%	340:340	arg1	NaCl					348:351	0-5 % (w/v) NaCl	336:351	0-5 % (w/v) NaCl	336:351	It grew at 4-40 °C (optimum 37 °C), at pH 3.0-9.0 (optimum, pH 7.0) and with 0-5 % (w/v) NaCl.
30156528	6	57	theme	strain	864:869	arg1	SYF10-1aT					871:879	strain SYF10-1aT	864:879	strain SYF10-1aT	864:879	The average nucleotide identity value and digital DNA-DNA hybridization value between strain SYF10-1aT and the most closely related species,L. crustorum LMG 23699T, were 80.5 and 33.3 %, respectively.
30156528	6	57	theme	strain	864:869	arg1	23699T					935:940	L. crustorum LMG 23699T	918:940	L. crustorum LMG 23699T	918:940	The average nucleotide identity value and digital DNA-DNA hybridization value between strain SYF10-1aT and the most closely related species,L. crustorum LMG 23699T, were 80.5 and 33.3 %, respectively.
30156528	3	58	dep	optimum	310:316	arg1	pH					319:320	pH 7.0	319:324	pH 7.0	319:324	It grew at 4-40 °C (optimum 37 °C), at pH 3.0-9.0 (optimum, pH 7.0) and with 0-5 % (w/v) NaCl.
30156528	8	59	theme	Polar	1122:1126	arg1	lipids					1128:1133	Polar lipids	1122:1133	Polar lipids	1122:1133	Polar lipids were diphosphatidylglycerol, phosphatidylglycerol, two unidentified aminolipids and two unidentified glycolipids.
30156528	11	60	theme	strain	1422:1427	arg1	SYF10-1aT					1429:1437	strain SYF10-1aT	1422:1437	strain SYF10-1aT	1422:1437	On the basis of this taxonomic study, strain SYF10-1aT represents a novel species within the genus Lactobacillus, for which the name Lactobacillus nuruki sp.
30156528	11	61	theme	taxonomic	1405:1413	arg1	study					1415:1419	this taxonomic study	1400:1419	this taxonomic study	1400:1419	On the basis of this taxonomic study, strain SYF10-1aT represents a novel species within the genus Lactobacillus, for which the name Lactobacillus nuruki sp.
30156528	9	62	theme	cell-wall	1253:1261	arg1	peptidoglycan					1263:1275	The cell-wall peptidoglycan	1249:1275	The cell-wall peptidoglycan	1249:1275	The cell-wall peptidoglycan was of the A4α type with an interpeptide bridge comprising l-Lys-d-Asp.
30156528	4	63	theme	Lactobacillus	529:541	arg1	23699T					557:562	Lactobacillus crustorum LMG 23699T	529:562	Lactobacillus crustorum LMG 23699T	529:562	Phylogenetic analysis using 16S rRNA gene sequences revealed that strain SYF10-1aT belonged to the genus Lactobacillus and showed the highest sequence similarity of 98.7 % to Lactobacillus crustorum LMG 23699T.
30156528	3	64	theme	optimum	279:285	arg1	4-40 °C					270:276	4-40 °C	270:276	4-40 °C (optimum 37 °C)	270:292	It grew at 4-40 °C (optimum 37 °C), at pH 3.0-9.0 (optimum, pH 7.0) and with 0-5 % (w/v) NaCl.
30156528	3	64	theme	optimum	279:285	arg1	37 °C					287:291	optimum 37 °C	279:291	optimum 37 °C	279:291	It grew at 4-40 °C (optimum 37 °C), at pH 3.0-9.0 (optimum, pH 7.0) and with 0-5 % (w/v) NaCl.
30156528	4	65	theme	Phylogenetic	354:365	arg1	analysis					367:374	Phylogenetic analysis	354:374	Phylogenetic analysis using 16S rRNA gene sequences	354:404	Phylogenetic analysis using 16S rRNA gene sequences revealed that strain SYF10-1aT belonged to the genus Lactobacillus and showed the highest sequence similarity of 98.7 % to Lactobacillus crustorum LMG 23699T.
30156528	11	66	theme	novel	1452:1456	arg1	species					1458:1464	a novel species	1450:1464	a novel species	1450:1464	On the basis of this taxonomic study, strain SYF10-1aT represents a novel species within the genus Lactobacillus, for which the name Lactobacillus nuruki sp.
30156528	10	67	theme	DNA	1353:1355	arg1	content					1361:1367	The DNA G+C content	1349:1367	The DNA G+C content	1349:1367	The DNA G+C content was 34.2 mol%.
30156528	10	67	theme	DNA	1353:1355	arg1	%					1381:1381	34.2 mol%	1373:1381	34.2 mol%	1373:1381	The DNA G+C content was 34.2 mol%.
30156528	6	68	theme	80.5	948:951	arg1	%					962:962	80.5 and 33.3 %	948:962	%	962:962	The average nucleotide identity value and digital DNA-DNA hybridization value between strain SYF10-1aT and the most closely related species,L. crustorum LMG 23699T, were 80.5 and 33.3 %, respectively.
30156528	6	68	theme	80.5	948:951	arg1	identity					801:808	The average nucleotide identity value and digital DNA-DNA hybridization value	778:854	The average nucleotide identity value and digital DNA-DNA hybridization value between strain SYF10-1aT and the most closely related species,L. crustorum LMG 23699T,	778:941	The average nucleotide identity value and digital DNA-DNA hybridization value between strain SYF10-1aT and the most closely related species,L. crustorum LMG 23699T, were 80.5 and 33.3 %, respectively.
30156528	2	69	theme	fermentation	237:248	arg1	Nuruk					209:213	Nuruk	209:213	Nuruk	209:213	A rod-shaped, Gram-stain-positive, non-flagellated, facultatively anaerobic bacterium, designated SYF10-1aT, was isolated from Nuruk, a Korean traditional fermentation starter.
30156528	2	69	theme	fermentation	237:248	arg1	starter					250:256	a Korean traditional fermentation starter	216:256	a Korean traditional fermentation starter	216:256	A rod-shaped, Gram-stain-positive, non-flagellated, facultatively anaerobic bacterium, designated SYF10-1aT, was isolated from Nuruk, a Korean traditional fermentation starter.
30156528	4	70	theme	LMG	553:555	arg1	23699T					557:562	Lactobacillus crustorum LMG 23699T	529:562	Lactobacillus crustorum LMG 23699T	529:562	Phylogenetic analysis using 16S rRNA gene sequences revealed that strain SYF10-1aT belonged to the genus Lactobacillus and showed the highest sequence similarity of 98.7 % to Lactobacillus crustorum LMG 23699T.
30156528	5	71	theme	Lactobacillus	703:715	arg1	radius					683:688	the radius	679:688	the radius of the genus Lactobacillus	679:715	A comparison of two housekeeping genes, pheS and rpoA, supported the suggestion that strain SYF10-1aT fell within the radius of the genus Lactobacillus, but was clearly separated from its closest related species.
30156528	2	72	theme	traditional	225:235	arg1	Nuruk					209:213	Nuruk	209:213	Nuruk	209:213	A rod-shaped, Gram-stain-positive, non-flagellated, facultatively anaerobic bacterium, designated SYF10-1aT, was isolated from Nuruk, a Korean traditional fermentation starter.
30156528	2	72	theme	traditional	225:235	arg1	starter					250:256	a Korean traditional fermentation starter	216:256	a Korean traditional fermentation starter	216:256	A rod-shaped, Gram-stain-positive, non-flagellated, facultatively anaerobic bacterium, designated SYF10-1aT, was isolated from Nuruk, a Korean traditional fermentation starter.
30156528	6	73	dep	identity	801:808	arg1	value					810:814	value	810:814	value	810:814	The average nucleotide identity value and digital DNA-DNA hybridization value between strain SYF10-1aT and the most closely related species,L. crustorum LMG 23699T, were 80.5 and 33.3 %, respectively.
30156528	6	73	dep	identity	801:808	arg1	value					850:854	digital DNA-DNA hybridization value	820:854	digital DNA-DNA hybridization value	820:854	The average nucleotide identity value and digital DNA-DNA hybridization value between strain SYF10-1aT and the most closely related species,L. crustorum LMG 23699T, were 80.5 and 33.3 %, respectively.
30156528	11	74	dep	study	1415:1419	arg1	basis					1391:1395	basis	1391:1395	basis	1391:1395	On the basis of this taxonomic study, strain SYF10-1aT represents a novel species within the genus Lactobacillus, for which the name Lactobacillus nuruki sp.
30156528	11	74	dep	study	1415:1419	arg1	the					1387:1389	the	1387:1389	the	1387:1389	On the basis of this taxonomic study, strain SYF10-1aT represents a novel species within the genus Lactobacillus, for which the name Lactobacillus nuruki sp.
30156528	4	75	theme	crustorum	543:551	arg1	23699T					557:562	Lactobacillus crustorum LMG 23699T	529:562	Lactobacillus crustorum LMG 23699T	529:562	Phylogenetic analysis using 16S rRNA gene sequences revealed that strain SYF10-1aT belonged to the genus Lactobacillus and showed the highest sequence similarity of 98.7 % to Lactobacillus crustorum LMG 23699T.
30017045	7	0	theme	drip	1334:1337	arg1	loss48 h					1339:1346	drip loss48 h	1334:1346	drip loss48 h	1334:1346	Cyclic HS increased drip loss48 h, cooking loss, malondialdehyde accumulation, and moisture content, whereas decreased pH24 h value, glutathione peroxidase (GSH-Px) activity, and crude protein content in the breast muscle (P < 0.05).
30017045	3	1	theme	MOS	609:611	arg1	group					613:617	the MOS group	605:617	the MOS group	605:617	Broilers in the control and HS groups were fed a basal diet, and in the MOS group were given the basal diet supplemented with 1 g/kg MOS for 42 days, respectively.
30017045	1	2	from	quality	313:319	arg1	level					276:280	serum corticosterone level	255:280	serum corticosterone level	255:280	This study investigated the effects of dietary mannan oligosaccharide (MOS) supplementation on growth performance, serum corticosterone level, and antioxidant ability, meat quality as well as chemical composition of breast muscle in broilers exposed to cyclic heat stress (HS).
30017045	1	2	from	quality	313:319	arg1	ability					299:305	antioxidant ability	287:305	antioxidant ability	287:305	This study investigated the effects of dietary mannan oligosaccharide (MOS) supplementation on growth performance, serum corticosterone level, and antioxidant ability, meat quality as well as chemical composition of breast muscle in broilers exposed to cyclic heat stress (HS).
30017045	1	2	from	quality	313:319	arg1	performance					242:252	growth performance	235:252	growth performance	235:252	This study investigated the effects of dietary mannan oligosaccharide (MOS) supplementation on growth performance, serum corticosterone level, and antioxidant ability, meat quality as well as chemical composition of breast muscle in broilers exposed to cyclic heat stress (HS).
30017045	1	3	theme	corticosterone	261:274	arg1	level					276:280	serum corticosterone level	255:280	serum corticosterone level	255:280	This study investigated the effects of dietary mannan oligosaccharide (MOS) supplementation on growth performance, serum corticosterone level, and antioxidant ability, meat quality as well as chemical composition of breast muscle in broilers exposed to cyclic heat stress (HS).
30017045	0	4	theme	Dietary	0:6	arg1	oligosaccharide					15:29	Dietary mannan oligosaccharide	0:29	Dietary mannan oligosaccharide	0:29	Dietary mannan oligosaccharide improves growth performance, muscle oxidative status, and meat quality in broilers under cyclic heat stress.
30017045	4	5	dep	the	1001:1003	arg1	both					1005:1008	both	1005:1008	both	1005:1008	The temperature was maintained 32-33 °C for the first 3 days for all broilers, and it was gradually reduced by 3 °C per week to a final temperature of 20 °C in the control group, whereas it remained unchanged for 8 h and reduced to the same level to the control group for the remaining 16 h daily in the both HS and MOS groups.
30017045	1	6	theme	dietary	179:185	arg1	supplementation					216:230	dietary mannan oligosaccharide (MOS) supplementation	179:230	dietary mannan oligosaccharide (MOS) supplementation	179:230	This study investigated the effects of dietary mannan oligosaccharide (MOS) supplementation on growth performance, serum corticosterone level, and antioxidant ability, meat quality as well as chemical composition of breast muscle in broilers exposed to cyclic heat stress (HS).
30017045	9	7	theme	dietary	1759:1765	arg1	supplementation					1771:1785	dietary MOS supplementation	1759:1785	dietary MOS supplementation	1759:1785	The results indicated that dietary MOS supplementation could improve growth performance, and oxidative status and meat quality of breast muscle in broilers under cyclic HS.
30017045	8	8	theme	dietary	1561:1567	arg1	supplementation					1573:1587	dietary MOS supplementation	1561:1587	dietary MOS supplementation	1561:1587	In contrast, dietary MOS supplementation reduced drip loss48 h and malondialdehyde concentration, and increased GSH-Px activity in breast muscle compared with the HS group (P < 0.05).
30017045	1	9	theme	oligosaccharide	194:208	arg1	supplementation					216:230	dietary mannan oligosaccharide (MOS) supplementation	179:230	dietary mannan oligosaccharide (MOS) supplementation	179:230	This study investigated the effects of dietary mannan oligosaccharide (MOS) supplementation on growth performance, serum corticosterone level, and antioxidant ability, meat quality as well as chemical composition of breast muscle in broilers exposed to cyclic heat stress (HS).
30017045	5	10	theme	corticosterone	1132:1145	arg1	P < 0.05					1154:1161	P < 0.05	1154:1161	P < 0.05	1154:1161	Compared with the control group, cyclic HS resulted in retarded growth performance and increased serum corticosterone level (P < 0.05).
30017045	5	10	theme	corticosterone	1132:1145	arg1	level					1147:1151	increased serum corticosterone level (P < 0.05)	1116:1162	increased serum corticosterone level (P < 0.05)	1116:1162	Compared with the control group, cyclic HS resulted in retarded growth performance and increased serum corticosterone level (P < 0.05).
30017045	0	11	theme	heat	127:130	arg1	stress					132:137	cyclic heat stress	120:137	cyclic heat stress	120:137	Dietary mannan oligosaccharide improves growth performance, muscle oxidative status, and meat quality in broilers under cyclic heat stress.
30017045	3	12	theme	control	553:559	arg1	groups					568:573	the control and HS groups	549:573	groups	568:573	Broilers in the control and HS groups were fed a basal diet, and in the MOS group were given the basal diet supplemented with 1 g/kg MOS for 42 days, respectively.
30017045	1	13	theme	MOS	211:213	arg1	supplementation					216:230	dietary mannan oligosaccharide (MOS) supplementation	179:230	dietary mannan oligosaccharide (MOS) supplementation	179:230	This study investigated the effects of dietary mannan oligosaccharide (MOS) supplementation on growth performance, serum corticosterone level, and antioxidant ability, meat quality as well as chemical composition of breast muscle in broilers exposed to cyclic heat stress (HS).
30017045	1	14	theme	cyclic	393:398	arg1	HS					413:414	HS	413:414	HS	413:414	This study investigated the effects of dietary mannan oligosaccharide (MOS) supplementation on growth performance, serum corticosterone level, and antioxidant ability, meat quality as well as chemical composition of breast muscle in broilers exposed to cyclic heat stress (HS).
30017045	1	14	theme	cyclic	393:398	arg1	stress					405:410	cyclic heat stress	393:410	cyclic heat stress (HS)	393:415	This study investigated the effects of dietary mannan oligosaccharide (MOS) supplementation on growth performance, serum corticosterone level, and antioxidant ability, meat quality as well as chemical composition of breast muscle in broilers exposed to cyclic heat stress (HS).
30017045	8	15	theme	drip	1597:1600	arg1	loss48 h					1602:1609	drip loss48 h	1597:1609	drip loss48 h	1597:1609	In contrast, dietary MOS supplementation reduced drip loss48 h and malondialdehyde concentration, and increased GSH-Px activity in breast muscle compared with the HS group (P < 0.05).
30017045	7	16	theme	glutathione	1447:1457	arg1	peroxidase					1459:1468	glutathione peroxidase	1447:1468	glutathione peroxidase (GSH-Px) activity	1447:1486	Cyclic HS increased drip loss48 h, cooking loss, malondialdehyde accumulation, and moisture content, whereas decreased pH24 h value, glutathione peroxidase (GSH-Px) activity, and crude protein content in the breast muscle (P < 0.05).
30017045	7	16	theme	glutathione	1447:1457	arg1	GSH-Px					1471:1476	GSH-Px	1471:1476	GSH-Px	1471:1476	Cyclic HS increased drip loss48 h, cooking loss, malondialdehyde accumulation, and moisture content, whereas decreased pH24 h value, glutathione peroxidase (GSH-Px) activity, and crude protein content in the breast muscle (P < 0.05).
30017045	4	17	theme	20 °C	852:856	arg1	temperature					837:847	a final temperature	829:847	a final temperature of 20 °C	829:856	The temperature was maintained 32-33 °C for the first 3 days for all broilers, and it was gradually reduced by 3 °C per week to a final temperature of 20 °C in the control group, whereas it remained unchanged for 8 h and reduced to the same level to the control group for the remaining 16 h daily in the both HS and MOS groups.
30017045	5	18	theme	serum	1126:1130	arg1	P < 0.05					1154:1161	P < 0.05	1154:1161	P < 0.05	1154:1161	Compared with the control group, cyclic HS resulted in retarded growth performance and increased serum corticosterone level (P < 0.05).
30017045	5	18	theme	serum	1126:1130	arg1	level					1147:1151	increased serum corticosterone level (P < 0.05)	1116:1162	increased serum corticosterone level (P < 0.05)	1116:1162	Compared with the control group, cyclic HS resulted in retarded growth performance and increased serum corticosterone level (P < 0.05).
30017045	0	19	theme	oxidative	67:75	arg1	status					77:82	muscle oxidative status	60:82	muscle oxidative status	60:82	Dietary mannan oligosaccharide improves growth performance, muscle oxidative status, and meat quality in broilers under cyclic heat stress.
30017045	3	20	theme	HS	565:566	arg1	groups					568:573	the control and HS groups	549:573	groups	568:573	Broilers in the control and HS groups were fed a basal diet, and in the MOS group were given the basal diet supplemented with 1 g/kg MOS for 42 days, respectively.
30017045	2	21	theme	Arbor	455:459	arg1	plus					467:470	Arbor Acres plus	455:470	Arbor Acres plus	455:470	144 one-day-old male broiler chicks (Arbor Acres plus) were randomly allocated to 3 treatments with 6 replicates each.
30017045	2	21	theme	Arbor	455:459	arg1	chicks					447:452	144 one-day-old male broiler chicks	418:452	144 one-day-old male broiler chicks (Arbor Acres plus)	418:471	144 one-day-old male broiler chicks (Arbor Acres plus) were randomly allocated to 3 treatments with 6 replicates each.
30017045	9	22	theme	meat	1846:1849	arg1	quality					1851:1857	meat quality	1846:1857	meat quality	1846:1857	The results indicated that dietary MOS supplementation could improve growth performance, and oxidative status and meat quality of breast muscle in broilers under cyclic HS.
30017045	5	23	theme	growth	1093:1098	arg1	performance					1100:1110	retarded growth performance	1084:1110	retarded growth performance	1084:1110	Compared with the control group, cyclic HS resulted in retarded growth performance and increased serum corticosterone level (P < 0.05).
30017045	0	24	theme	meat	89:92	arg1	quality					94:100	meat quality	89:100	meat quality	89:100	Dietary mannan oligosaccharide improves growth performance, muscle oxidative status, and meat quality in broilers under cyclic heat stress.
30017045	8	25	theme	GSH-Px	1660:1665	arg1	activity					1667:1674	increased GSH-Px activity	1650:1674	increased GSH-Px activity	1650:1674	In contrast, dietary MOS supplementation reduced drip loss48 h and malondialdehyde concentration, and increased GSH-Px activity in breast muscle compared with the HS group (P < 0.05).
30017045	7	26	theme	breast	1522:1527	arg1	muscle					1529:1534	the breast muscle	1518:1534	the breast muscle	1518:1534	Cyclic HS increased drip loss48 h, cooking loss, malondialdehyde accumulation, and moisture content, whereas decreased pH24 h value, glutathione peroxidase (GSH-Px) activity, and crude protein content in the breast muscle (P < 0.05).
30017045	7	27	theme	moisture	1397:1404	arg1	content					1406:1412	moisture content	1397:1412	moisture content	1397:1412	Cyclic HS increased drip loss48 h, cooking loss, malondialdehyde accumulation, and moisture content, whereas decreased pH24 h value, glutathione peroxidase (GSH-Px) activity, and crude protein content in the breast muscle (P < 0.05).
30017045	5	28	theme	control	1047:1053	arg1	group					1055:1059	the control group	1043:1059	the control group	1043:1059	Compared with the control group, cyclic HS resulted in retarded growth performance and increased serum corticosterone level (P < 0.05).
30017045	1	29	theme	breast	356:361	arg1	muscle					363:368	breast muscle	356:368	breast muscle	356:368	This study investigated the effects of dietary mannan oligosaccharide (MOS) supplementation on growth performance, serum corticosterone level, and antioxidant ability, meat quality as well as chemical composition of breast muscle in broilers exposed to cyclic heat stress (HS).
30017045	9	30	from	status	1835:1840	arg1	broilers					1879:1886	broilers	1879:1886	broilers	1879:1886	The results indicated that dietary MOS supplementation could improve growth performance, and oxidative status and meat quality of breast muscle in broilers under cyclic HS.
30017045	9	31	theme	muscle	1869:1874	arg1	quality					1851:1857	meat quality	1846:1857	meat quality	1846:1857	The results indicated that dietary MOS supplementation could improve growth performance, and oxidative status and meat quality of breast muscle in broilers under cyclic HS.
30017045	9	31	theme	muscle	1869:1874	arg1	performance					1808:1818	growth performance	1801:1818	growth performance	1801:1818	The results indicated that dietary MOS supplementation could improve growth performance, and oxidative status and meat quality of breast muscle in broilers under cyclic HS.
30017045	9	31	theme	muscle	1869:1874	arg1	status					1835:1840	oxidative status	1825:1840	oxidative status	1825:1840	The results indicated that dietary MOS supplementation could improve growth performance, and oxidative status and meat quality of breast muscle in broilers under cyclic HS.
30017045	6	32	theme	cyclic	1292:1297	arg1	P < 0.05					1303:1310	P < 0.05	1303:1310	P < 0.05	1303:1310	The supplementation of MOS promoted growth performance and reduced serum corticosterone concentration in broilers subjected to cyclic HS (P < 0.05).
30017045	6	32	theme	cyclic	1292:1297	arg1	HS					1299:1300	cyclic HS	1292:1300	cyclic HS (P < 0.05)	1292:1311	The supplementation of MOS promoted growth performance and reduced serum corticosterone concentration in broilers subjected to cyclic HS (P < 0.05).
30017045	7	33	theme	protein	1499:1505	arg1	content					1507:1513	crude protein content	1493:1513	crude protein content	1493:1513	Cyclic HS increased drip loss48 h, cooking loss, malondialdehyde accumulation, and moisture content, whereas decreased pH24 h value, glutathione peroxidase (GSH-Px) activity, and crude protein content in the breast muscle (P < 0.05).
30017045	7	34	dep	increased	1324:1332	arg1	whereas					1415:1421	whereas	1415:1421	whereas	1415:1421	Cyclic HS increased drip loss48 h, cooking loss, malondialdehyde accumulation, and moisture content, whereas decreased pH24 h value, glutathione peroxidase (GSH-Px) activity, and crude protein content in the breast muscle (P < 0.05).
30017045	9	35	from	performance	1808:1818	arg1	broilers					1879:1886	broilers	1879:1886	broilers	1879:1886	The results indicated that dietary MOS supplementation could improve growth performance, and oxidative status and meat quality of breast muscle in broilers under cyclic HS.
30017045	9	36	theme	cyclic	1894:1899	arg1	HS					1901:1902	cyclic HS	1894:1902	cyclic HS	1894:1902	The results indicated that dietary MOS supplementation could improve growth performance, and oxidative status and meat quality of breast muscle in broilers under cyclic HS.
30017045	0	37	theme	growth	40:45	arg1	performance					47:57	growth performance	40:57	growth performance	40:57	Dietary mannan oligosaccharide improves growth performance, muscle oxidative status, and meat quality in broilers under cyclic heat stress.
30017045	2	38	theme	broiler	439:445	arg1	plus					467:470	Arbor Acres plus	455:470	Arbor Acres plus	455:470	144 one-day-old male broiler chicks (Arbor Acres plus) were randomly allocated to 3 treatments with 6 replicates each.
30017045	2	38	theme	broiler	439:445	arg1	chicks					447:452	144 one-day-old male broiler chicks	418:452	144 one-day-old male broiler chicks (Arbor Acres plus)	418:471	144 one-day-old male broiler chicks (Arbor Acres plus) were randomly allocated to 3 treatments with 6 replicates each.
30017045	4	39	from	groups	1021:1026	arg1	daily					992:996	daily	992:996	daily	992:996	The temperature was maintained 32-33 °C for the first 3 days for all broilers, and it was gradually reduced by 3 °C per week to a final temperature of 20 °C in the control group, whereas it remained unchanged for 8 h and reduced to the same level to the control group for the remaining 16 h daily in the both HS and MOS groups.
30017045	2	40	theme	male	434:437	arg1	plus					467:470	Arbor Acres plus	455:470	Arbor Acres plus	455:470	144 one-day-old male broiler chicks (Arbor Acres plus) were randomly allocated to 3 treatments with 6 replicates each.
30017045	2	40	theme	male	434:437	arg1	chicks					447:452	144 one-day-old male broiler chicks	418:452	144 one-day-old male broiler chicks (Arbor Acres plus)	418:471	144 one-day-old male broiler chicks (Arbor Acres plus) were randomly allocated to 3 treatments with 6 replicates each.
30017045	6	41	theme	corticosterone	1238:1251	arg1	concentration					1253:1265	serum corticosterone concentration	1232:1265	serum corticosterone concentration	1232:1265	The supplementation of MOS promoted growth performance and reduced serum corticosterone concentration in broilers subjected to cyclic HS (P < 0.05).
30017045	1	42	theme	meat	308:311	arg1	quality					313:319	meat quality	308:319	meat quality	308:319	This study investigated the effects of dietary mannan oligosaccharide (MOS) supplementation on growth performance, serum corticosterone level, and antioxidant ability, meat quality as well as chemical composition of breast muscle in broilers exposed to cyclic heat stress (HS).
30017045	7	43	theme	cooking	1349:1355	arg1	loss					1357:1360	cooking loss	1349:1360	cooking loss	1349:1360	Cyclic HS increased drip loss48 h, cooking loss, malondialdehyde accumulation, and moisture content, whereas decreased pH24 h value, glutathione peroxidase (GSH-Px) activity, and crude protein content in the breast muscle (P < 0.05).
30017045	4	44	theme	same	937:940	arg1	level					942:946	the same level	933:946	the same level	933:946	The temperature was maintained 32-33 °C for the first 3 days for all broilers, and it was gradually reduced by 3 °C per week to a final temperature of 20 °C in the control group, whereas it remained unchanged for 8 h and reduced to the same level to the control group for the remaining 16 h daily in the both HS and MOS groups.
30017045	2	45	with	treatments	502:511	arg1	6					518:518	6	518:518	6	518:518	144 one-day-old male broiler chicks (Arbor Acres plus) were randomly allocated to 3 treatments with 6 replicates each.
30017045	5	46	theme	cyclic	1062:1067	arg1	HS					1069:1070	cyclic HS	1062:1070	cyclic HS	1062:1070	Compared with the control group, cyclic HS resulted in retarded growth performance and increased serum corticosterone level (P < 0.05).
30017045	2	47	dep	6	518:518	arg1	replicates					520:529	replicates	520:529	replicates each	520:534	144 one-day-old male broiler chicks (Arbor Acres plus) were randomly allocated to 3 treatments with 6 replicates each.
30017045	7	48	theme	Cyclic	1314:1319	arg1	HS					1321:1322	Cyclic HS	1314:1322	Cyclic HS	1314:1322	Cyclic HS increased drip loss48 h, cooking loss, malondialdehyde accumulation, and moisture content, whereas decreased pH24 h value, glutathione peroxidase (GSH-Px) activity, and crude protein content in the breast muscle (P < 0.05).
30017045	8	49	theme	HS	1711:1712	arg1	P < 0.05					1721:1728	P < 0.05	1721:1728	P < 0.05	1721:1728	In contrast, dietary MOS supplementation reduced drip loss48 h and malondialdehyde concentration, and increased GSH-Px activity in breast muscle compared with the HS group (P < 0.05).
30017045	8	49	theme	HS	1711:1712	arg1	group					1714:1718	the HS group	1707:1718	the HS group (P < 0.05)	1707:1729	In contrast, dietary MOS supplementation reduced drip loss48 h and malondialdehyde concentration, and increased GSH-Px activity in breast muscle compared with the HS group (P < 0.05).
30017045	8	50	theme	malondialdehyde	1615:1629	arg1	concentration					1631:1643	malondialdehyde concentration	1615:1643	malondialdehyde concentration	1615:1643	In contrast, dietary MOS supplementation reduced drip loss48 h and malondialdehyde concentration, and increased GSH-Px activity in breast muscle compared with the HS group (P < 0.05).
30017045	1	51	theme	serum	255:259	arg1	level					276:280	serum corticosterone level	255:280	serum corticosterone level	255:280	This study investigated the effects of dietary mannan oligosaccharide (MOS) supplementation on growth performance, serum corticosterone level, and antioxidant ability, meat quality as well as chemical composition of breast muscle in broilers exposed to cyclic heat stress (HS).
30017045	4	52	theme	remaining	977:985	arg1	16 h					987:990	the remaining 16 h	973:990	the remaining 16 h daily in the both HS and MOS groups	973:1026	The temperature was maintained 32-33 °C for the first 3 days for all broilers, and it was gradually reduced by 3 °C per week to a final temperature of 20 °C in the control group, whereas it remained unchanged for 8 h and reduced to the same level to the control group for the remaining 16 h daily in the both HS and MOS groups.
30017045	4	53	from	daily	992:996	arg1	HS					1010:1011	the both HS and MOS groups	1001:1026	HS	1010:1011	The temperature was maintained 32-33 °C for the first 3 days for all broilers, and it was gradually reduced by 3 °C per week to a final temperature of 20 °C in the control group, whereas it remained unchanged for 8 h and reduced to the same level to the control group for the remaining 16 h daily in the both HS and MOS groups.
30017045	4	53	from	daily	992:996	arg1	groups					1021:1026	the both HS and MOS groups	1001:1026	groups	1021:1026	The temperature was maintained 32-33 °C for the first 3 days for all broilers, and it was gradually reduced by 3 °C per week to a final temperature of 20 °C in the control group, whereas it remained unchanged for 8 h and reduced to the same level to the control group for the remaining 16 h daily in the both HS and MOS groups.
30017045	4	53	from	daily	992:996	arg1	the					1001:1003	the	1001:1003	the	1001:1003	The temperature was maintained 32-33 °C for the first 3 days for all broilers, and it was gradually reduced by 3 °C per week to a final temperature of 20 °C in the control group, whereas it remained unchanged for 8 h and reduced to the same level to the control group for the remaining 16 h daily in the both HS and MOS groups.
30017045	4	54	theme	first	749:753	arg1	days					757:760	the first 3 days	745:760	the first 3 days for all broilers	745:777	The temperature was maintained 32-33 °C for the first 3 days for all broilers, and it was gradually reduced by 3 °C per week to a final temperature of 20 °C in the control group, whereas it remained unchanged for 8 h and reduced to the same level to the control group for the remaining 16 h daily in the both HS and MOS groups.
30017045	4	55	theme	daily	992:996	arg1	16 h					987:990	the remaining 16 h	973:990	the remaining 16 h daily in the both HS and MOS groups	973:1026	The temperature was maintained 32-33 °C for the first 3 days for all broilers, and it was gradually reduced by 3 °C per week to a final temperature of 20 °C in the control group, whereas it remained unchanged for 8 h and reduced to the same level to the control group for the remaining 16 h daily in the both HS and MOS groups.
30017045	3	56	theme	basal	634:638	arg1	diet					640:643	the basal diet	630:643	the basal diet supplemented with 1 g/kg MOS	630:672	Broilers in the control and HS groups were fed a basal diet, and in the MOS group were given the basal diet supplemented with 1 g/kg MOS for 42 days, respectively.
30017045	4	57	theme	control	955:961	arg1	group					963:967	the control group	951:967	the control group for the remaining 16 h daily in the both HS and MOS groups	951:1026	The temperature was maintained 32-33 °C for the first 3 days for all broilers, and it was gradually reduced by 3 °C per week to a final temperature of 20 °C in the control group, whereas it remained unchanged for 8 h and reduced to the same level to the control group for the remaining 16 h daily in the both HS and MOS groups.
30017045	0	58	theme	cyclic	120:125	arg1	stress					132:137	cyclic heat stress	120:137	cyclic heat stress	120:137	Dietary mannan oligosaccharide improves growth performance, muscle oxidative status, and meat quality in broilers under cyclic heat stress.
30017045	1	59	theme	mannan	187:192	arg1	supplementation					216:230	dietary mannan oligosaccharide (MOS) supplementation	179:230	dietary mannan oligosaccharide (MOS) supplementation	179:230	This study investigated the effects of dietary mannan oligosaccharide (MOS) supplementation on growth performance, serum corticosterone level, and antioxidant ability, meat quality as well as chemical composition of breast muscle in broilers exposed to cyclic heat stress (HS).
30017045	9	60	theme	MOS	1767:1769	arg1	supplementation					1771:1785	dietary MOS supplementation	1759:1785	dietary MOS supplementation	1759:1785	The results indicated that dietary MOS supplementation could improve growth performance, and oxidative status and meat quality of breast muscle in broilers under cyclic HS.
30017045	3	61	theme	1 g/kg	663:668	arg1	MOS					670:672	1 g/kg MOS	663:672	1 g/kg MOS	663:672	Broilers in the control and HS groups were fed a basal diet, and in the MOS group were given the basal diet supplemented with 1 g/kg MOS for 42 days, respectively.
30017045	7	62	dep	decreased	1423:1431	arg1	P < 0.05					1537:1544	P < 0.05	1537:1544	P < 0.05	1537:1544	Cyclic HS increased drip loss48 h, cooking loss, malondialdehyde accumulation, and moisture content, whereas decreased pH24 h value, glutathione peroxidase (GSH-Px) activity, and crude protein content in the breast muscle (P < 0.05).
30017045	7	63	theme	peroxidase	1459:1468	arg1	activity					1479:1486	glutathione peroxidase (GSH-Px) activity	1447:1486	glutathione peroxidase (GSH-Px) activity	1447:1486	Cyclic HS increased drip loss48 h, cooking loss, malondialdehyde accumulation, and moisture content, whereas decreased pH24 h value, glutathione peroxidase (GSH-Px) activity, and crude protein content in the breast muscle (P < 0.05).
30017045	0	64	from	status	77:82	arg1	broilers					105:112	broilers	105:112	broilers	105:112	Dietary mannan oligosaccharide improves growth performance, muscle oxidative status, and meat quality in broilers under cyclic heat stress.
30017045	4	65	theme	MOS	1017:1019	arg1	groups					1021:1026	the both HS and MOS groups	1001:1026	groups	1021:1026	The temperature was maintained 32-33 °C for the first 3 days for all broilers, and it was gradually reduced by 3 °C per week to a final temperature of 20 °C in the control group, whereas it remained unchanged for 8 h and reduced to the same level to the control group for the remaining 16 h daily in the both HS and MOS groups.
30017045	8	66	theme	MOS	1569:1571	arg1	supplementation					1573:1587	dietary MOS supplementation	1561:1587	dietary MOS supplementation	1561:1587	In contrast, dietary MOS supplementation reduced drip loss48 h and malondialdehyde concentration, and increased GSH-Px activity in breast muscle compared with the HS group (P < 0.05).
30017045	9	67	theme	growth	1801:1806	arg1	performance					1808:1818	growth performance	1801:1818	growth performance	1801:1818	The results indicated that dietary MOS supplementation could improve growth performance, and oxidative status and meat quality of breast muscle in broilers under cyclic HS.
30017045	1	68	theme	heat	400:403	arg1	HS					413:414	HS	413:414	HS	413:414	This study investigated the effects of dietary mannan oligosaccharide (MOS) supplementation on growth performance, serum corticosterone level, and antioxidant ability, meat quality as well as chemical composition of breast muscle in broilers exposed to cyclic heat stress (HS).
30017045	1	68	theme	heat	400:403	arg1	stress					405:410	cyclic heat stress	393:410	cyclic heat stress (HS)	393:415	This study investigated the effects of dietary mannan oligosaccharide (MOS) supplementation on growth performance, serum corticosterone level, and antioxidant ability, meat quality as well as chemical composition of breast muscle in broilers exposed to cyclic heat stress (HS).
30017045	7	69	theme	pH24 h	1433:1438	arg1	value					1440:1444	pH24 h value	1433:1444	pH24 h value	1433:1444	Cyclic HS increased drip loss48 h, cooking loss, malondialdehyde accumulation, and moisture content, whereas decreased pH24 h value, glutathione peroxidase (GSH-Px) activity, and crude protein content in the breast muscle (P < 0.05).
30017045	0	70	from	quality	94:100	arg1	broilers					105:112	broilers	105:112	broilers	105:112	Dietary mannan oligosaccharide improves growth performance, muscle oxidative status, and meat quality in broilers under cyclic heat stress.
30017045	5	71	theme	increased	1116:1124	arg1	P < 0.05					1154:1161	P < 0.05	1154:1161	P < 0.05	1154:1161	Compared with the control group, cyclic HS resulted in retarded growth performance and increased serum corticosterone level (P < 0.05).
30017045	5	71	theme	increased	1116:1124	arg1	level					1147:1151	increased serum corticosterone level (P < 0.05)	1116:1162	increased serum corticosterone level (P < 0.05)	1116:1162	Compared with the control group, cyclic HS resulted in retarded growth performance and increased serum corticosterone level (P < 0.05).
30017045	6	72	theme	serum	1232:1236	arg1	concentration					1253:1265	serum corticosterone concentration	1232:1265	serum corticosterone concentration	1232:1265	The supplementation of MOS promoted growth performance and reduced serum corticosterone concentration in broilers subjected to cyclic HS (P < 0.05).
30017045	2	73	theme	Acres	461:465	arg1	plus					467:470	Arbor Acres plus	455:470	Arbor Acres plus	455:470	144 one-day-old male broiler chicks (Arbor Acres plus) were randomly allocated to 3 treatments with 6 replicates each.
30017045	2	73	theme	Acres	461:465	arg1	chicks					447:452	144 one-day-old male broiler chicks	418:452	144 one-day-old male broiler chicks (Arbor Acres plus)	418:471	144 one-day-old male broiler chicks (Arbor Acres plus) were randomly allocated to 3 treatments with 6 replicates each.
30017045	9	74	theme	oxidative	1825:1833	arg1	status					1835:1840	oxidative status	1825:1840	oxidative status	1825:1840	The results indicated that dietary MOS supplementation could improve growth performance, and oxidative status and meat quality of breast muscle in broilers under cyclic HS.
30017045	1	75	from	effects	168:174	arg1	level					276:280	serum corticosterone level	255:280	serum corticosterone level	255:280	This study investigated the effects of dietary mannan oligosaccharide (MOS) supplementation on growth performance, serum corticosterone level, and antioxidant ability, meat quality as well as chemical composition of breast muscle in broilers exposed to cyclic heat stress (HS).
30017045	1	75	from	effects	168:174	arg1	ability					299:305	antioxidant ability	287:305	antioxidant ability	287:305	This study investigated the effects of dietary mannan oligosaccharide (MOS) supplementation on growth performance, serum corticosterone level, and antioxidant ability, meat quality as well as chemical composition of breast muscle in broilers exposed to cyclic heat stress (HS).
30017045	1	75	from	effects	168:174	arg1	performance					242:252	growth performance	235:252	growth performance	235:252	This study investigated the effects of dietary mannan oligosaccharide (MOS) supplementation on growth performance, serum corticosterone level, and antioxidant ability, meat quality as well as chemical composition of breast muscle in broilers exposed to cyclic heat stress (HS).
30017045	5	76	theme	retarded	1084:1091	arg1	performance					1100:1110	retarded growth performance	1084:1110	retarded growth performance	1084:1110	Compared with the control group, cyclic HS resulted in retarded growth performance and increased serum corticosterone level (P < 0.05).
30017045	6	77	theme	growth	1201:1206	arg1	performance					1208:1218	growth performance	1201:1218	growth performance	1201:1218	The supplementation of MOS promoted growth performance and reduced serum corticosterone concentration in broilers subjected to cyclic HS (P < 0.05).
30017045	1	78	theme	chemical	332:339	arg1	composition					341:351	chemical composition	332:351	chemical composition of breast muscle	332:368	This study investigated the effects of dietary mannan oligosaccharide (MOS) supplementation on growth performance, serum corticosterone level, and antioxidant ability, meat quality as well as chemical composition of breast muscle in broilers exposed to cyclic heat stress (HS).
30017045	6	79	theme	MOS	1188:1190	arg1	supplementation					1169:1183	The supplementation	1165:1183	The supplementation of MOS	1165:1190	The supplementation of MOS promoted growth performance and reduced serum corticosterone concentration in broilers subjected to cyclic HS (P < 0.05).
30017045	8	80	theme	increased	1650:1658	arg1	activity					1667:1674	increased GSH-Px activity	1650:1674	increased GSH-Px activity	1650:1674	In contrast, dietary MOS supplementation reduced drip loss48 h and malondialdehyde concentration, and increased GSH-Px activity in breast muscle compared with the HS group (P < 0.05).
30017045	4	81	theme	final	831:835	arg1	temperature					837:847	a final temperature	829:847	a final temperature of 20 °C	829:856	The temperature was maintained 32-33 °C for the first 3 days for all broilers, and it was gradually reduced by 3 °C per week to a final temperature of 20 °C in the control group, whereas it remained unchanged for 8 h and reduced to the same level to the control group for the remaining 16 h daily in the both HS and MOS groups.
30017045	0	82	from	performance	47:57	arg1	broilers					105:112	broilers	105:112	broilers	105:112	Dietary mannan oligosaccharide improves growth performance, muscle oxidative status, and meat quality in broilers under cyclic heat stress.
30017045	9	83	theme	breast	1862:1867	arg1	muscle					1869:1874	breast muscle	1862:1874	breast muscle	1862:1874	The results indicated that dietary MOS supplementation could improve growth performance, and oxidative status and meat quality of breast muscle in broilers under cyclic HS.
30017045	0	84	theme	mannan	8:13	arg1	oligosaccharide					15:29	Dietary mannan oligosaccharide	0:29	Dietary mannan oligosaccharide	0:29	Dietary mannan oligosaccharide improves growth performance, muscle oxidative status, and meat quality in broilers under cyclic heat stress.
30017045	1	85	theme	muscle	363:368	arg1	effects					168:174	the effects	164:174	the effects of dietary mannan oligosaccharide (MOS) supplementation	164:230	This study investigated the effects of dietary mannan oligosaccharide (MOS) supplementation on growth performance, serum corticosterone level, and antioxidant ability, meat quality as well as chemical composition of breast muscle in broilers exposed to cyclic heat stress (HS).
30017045	1	85	theme	muscle	363:368	arg1	composition					341:351	chemical composition	332:351	chemical composition of breast muscle	332:368	This study investigated the effects of dietary mannan oligosaccharide (MOS) supplementation on growth performance, serum corticosterone level, and antioxidant ability, meat quality as well as chemical composition of breast muscle in broilers exposed to cyclic heat stress (HS).
30017045	1	85	theme	muscle	363:368	arg1	quality					313:319	meat quality	308:319	meat quality	308:319	This study investigated the effects of dietary mannan oligosaccharide (MOS) supplementation on growth performance, serum corticosterone level, and antioxidant ability, meat quality as well as chemical composition of breast muscle in broilers exposed to cyclic heat stress (HS).
30017045	3	86	from	Broilers	537:544	arg1	groups					568:573	the control and HS groups	549:573	groups	568:573	Broilers in the control and HS groups were fed a basal diet, and in the MOS group were given the basal diet supplemented with 1 g/kg MOS for 42 days, respectively.
30017045	1	87	from	composition	341:351	arg1	level					276:280	serum corticosterone level	255:280	serum corticosterone level	255:280	This study investigated the effects of dietary mannan oligosaccharide (MOS) supplementation on growth performance, serum corticosterone level, and antioxidant ability, meat quality as well as chemical composition of breast muscle in broilers exposed to cyclic heat stress (HS).
30017045	1	87	from	composition	341:351	arg1	ability					299:305	antioxidant ability	287:305	antioxidant ability	287:305	This study investigated the effects of dietary mannan oligosaccharide (MOS) supplementation on growth performance, serum corticosterone level, and antioxidant ability, meat quality as well as chemical composition of breast muscle in broilers exposed to cyclic heat stress (HS).
30017045	1	87	from	composition	341:351	arg1	performance					242:252	growth performance	235:252	growth performance	235:252	This study investigated the effects of dietary mannan oligosaccharide (MOS) supplementation on growth performance, serum corticosterone level, and antioxidant ability, meat quality as well as chemical composition of breast muscle in broilers exposed to cyclic heat stress (HS).
30017045	8	88	theme	breast	1679:1684	arg1	muscle					1686:1691	breast muscle	1679:1691	breast muscle	1679:1691	In contrast, dietary MOS supplementation reduced drip loss48 h and malondialdehyde concentration, and increased GSH-Px activity in breast muscle compared with the HS group (P < 0.05).
30017045	3	89	theme	basal	586:590	arg1	diet					592:595	a basal diet	584:595	a basal diet	584:595	Broilers in the control and HS groups were fed a basal diet, and in the MOS group were given the basal diet supplemented with 1 g/kg MOS for 42 days, respectively.
30017045	1	90	theme	antioxidant	287:297	arg1	ability					299:305	antioxidant ability	287:305	antioxidant ability	287:305	This study investigated the effects of dietary mannan oligosaccharide (MOS) supplementation on growth performance, serum corticosterone level, and antioxidant ability, meat quality as well as chemical composition of breast muscle in broilers exposed to cyclic heat stress (HS).
30017045	7	91	theme	crude	1493:1497	arg1	content					1507:1513	crude protein content	1493:1513	crude protein content	1493:1513	Cyclic HS increased drip loss48 h, cooking loss, malondialdehyde accumulation, and moisture content, whereas decreased pH24 h value, glutathione peroxidase (GSH-Px) activity, and crude protein content in the breast muscle (P < 0.05).
30017045	4	92	theme	control	865:871	arg1	group					873:877	the control group	861:877	the control group	861:877	The temperature was maintained 32-33 °C for the first 3 days for all broilers, and it was gradually reduced by 3 °C per week to a final temperature of 20 °C in the control group, whereas it remained unchanged for 8 h and reduced to the same level to the control group for the remaining 16 h daily in the both HS and MOS groups.
30017045	0	93	theme	muscle	60:65	arg1	status					77:82	muscle oxidative status	60:82	muscle oxidative status	60:82	Dietary mannan oligosaccharide improves growth performance, muscle oxidative status, and meat quality in broilers under cyclic heat stress.
30017045	7	94	theme	malondialdehyde	1363:1377	arg1	accumulation					1379:1390	malondialdehyde accumulation	1363:1390	malondialdehyde accumulation	1363:1390	Cyclic HS increased drip loss48 h, cooking loss, malondialdehyde accumulation, and moisture content, whereas decreased pH24 h value, glutathione peroxidase (GSH-Px) activity, and crude protein content in the breast muscle (P < 0.05).
30017045	2	95	theme	one-day-old	422:432	arg1	plus					467:470	Arbor Acres plus	455:470	Arbor Acres plus	455:470	144 one-day-old male broiler chicks (Arbor Acres plus) were randomly allocated to 3 treatments with 6 replicates each.
30017045	2	95	theme	one-day-old	422:432	arg1	chicks					447:452	144 one-day-old male broiler chicks	418:452	144 one-day-old male broiler chicks (Arbor Acres plus)	418:471	144 one-day-old male broiler chicks (Arbor Acres plus) were randomly allocated to 3 treatments with 6 replicates each.
30017045	4	96	from	HS	1010:1011	arg1	daily					992:996	daily	992:996	daily	992:996	The temperature was maintained 32-33 °C for the first 3 days for all broilers, and it was gradually reduced by 3 °C per week to a final temperature of 20 °C in the control group, whereas it remained unchanged for 8 h and reduced to the same level to the control group for the remaining 16 h daily in the both HS and MOS groups.
30017045	1	97	theme	supplementation	216:230	arg1	effects					168:174	the effects	164:174	the effects of dietary mannan oligosaccharide (MOS) supplementation	164:230	This study investigated the effects of dietary mannan oligosaccharide (MOS) supplementation on growth performance, serum corticosterone level, and antioxidant ability, meat quality as well as chemical composition of breast muscle in broilers exposed to cyclic heat stress (HS).
30017045	1	97	theme	supplementation	216:230	arg1	composition					341:351	chemical composition	332:351	chemical composition of breast muscle	332:368	This study investigated the effects of dietary mannan oligosaccharide (MOS) supplementation on growth performance, serum corticosterone level, and antioxidant ability, meat quality as well as chemical composition of breast muscle in broilers exposed to cyclic heat stress (HS).
30017045	1	97	theme	supplementation	216:230	arg1	quality					313:319	meat quality	308:319	meat quality	308:319	This study investigated the effects of dietary mannan oligosaccharide (MOS) supplementation on growth performance, serum corticosterone level, and antioxidant ability, meat quality as well as chemical composition of breast muscle in broilers exposed to cyclic heat stress (HS).
30017045	4	98	from	the	1001:1003	arg1	daily					992:996	daily	992:996	daily	992:996	The temperature was maintained 32-33 °C for the first 3 days for all broilers, and it was gradually reduced by 3 °C per week to a final temperature of 20 °C in the control group, whereas it remained unchanged for 8 h and reduced to the same level to the control group for the remaining 16 h daily in the both HS and MOS groups.
30017045	9	99	from	quality	1851:1857	arg1	broilers					1879:1886	broilers	1879:1886	broilers	1879:1886	The results indicated that dietary MOS supplementation could improve growth performance, and oxidative status and meat quality of breast muscle in broilers under cyclic HS.
30017045	1	100	theme	growth	235:240	arg1	performance					242:252	growth performance	235:252	growth performance	235:252	This study investigated the effects of dietary mannan oligosaccharide (MOS) supplementation on growth performance, serum corticosterone level, and antioxidant ability, meat quality as well as chemical composition of breast muscle in broilers exposed to cyclic heat stress (HS).
29460474	9	0	located	observed	1246:1253	arg2	changes					1214:1220	most pronounced changes	1198:1220	most pronounced changes in gene expression	1198:1239	Adult insulin sensitivity and dyslipidaemia were improved and most pronounced changes in gene expression were observed in the ileum.
29460474	9	0	located	observed	1246:1253	arg1	ileum					1262:1266	the ileum	1258:1266	the ileum	1258:1266	Adult insulin sensitivity and dyslipidaemia were improved and most pronounced changes in gene expression were observed in the ileum.
29460474	6	1	theme	gut	793:795	arg1	composition					758:768	composition	758:768	composition	758:768	Body weight and composition were monitored, as was gut microbiota composition at PN21, 42 and 98.
29460474	6	1	theme	gut	793:795	arg1	composition					808:818	was gut microbiota composition	789:818	was gut microbiota composition at PN21, 42 and 98	789:837	Body weight and composition were monitored, as was gut microbiota composition at PN21, 42 and 98.
29460474	6	1	theme	gut	793:795	arg1	weight					747:752	Body weight	742:752	Body weight	742:752	Body weight and composition were monitored, as was gut microbiota composition at PN21, 42 and 98.
29460474	11	2	theme	status	1729:1734	arg1	protection					1698:1707	long-lasting protection	1685:1707	long-lasting protection of metabolic health status	1685:1734	Microbiota transplantation using samples collected from synbiotics-supplemented adolescent mice at PN42 to age-matched germ-free recipients did not transfer the beneficial phenotype, indicating that synbiotics-modified microbiota at PN42 is not sufficient to transfer long-lasting protection of metabolic health status.
29460474	12	3	theme	interventions	1830:1842	arg1	timing					1810:1815	timing	1810:1815	timing of synbiotic interventions	1810:1842	CONCLUSION Together, these findings show the potential and importance of timing of synbiotic interventions in early life during crucial microbiota development as a preventive measure to lower the risk of obesity and improve metabolic health throughout life.
29460474	11	4	theme	metabolic	1712:1720	arg1	status					1729:1734	metabolic health status	1712:1734	metabolic health status	1712:1734	Microbiota transplantation using samples collected from synbiotics-supplemented adolescent mice at PN42 to age-matched germ-free recipients did not transfer the beneficial phenotype, indicating that synbiotics-modified microbiota at PN42 is not sufficient to transfer long-lasting protection of metabolic health status.
29460474	12	5	dep	potential	1782:1790	arg1	the					1778:1780	the	1778:1780	the	1778:1780	CONCLUSION Together, these findings show the potential and importance of timing of synbiotic interventions in early life during crucial microbiota development as a preventive measure to lower the risk of obesity and improve metabolic health throughout life.
29460474	2	6	theme	life	243:246	arg1	perturbation					259:270	early life microbiome perturbation	237:270	early life microbiome perturbation	237:270	The symbiosis between host and microbiome is initiated at birth, and early life microbiome perturbation can disturb health throughout life.
29460474	12	7	theme	metabolic	1961:1969	arg1	health					1971:1976	metabolic health	1961:1976	metabolic health	1961:1976	CONCLUSION Together, these findings show the potential and importance of timing of synbiotic interventions in early life during crucial microbiota development as a preventive measure to lower the risk of obesity and improve metabolic health throughout life.
29460474	9	8	theme	pronounced	1203:1212	arg1	changes					1214:1220	most pronounced changes	1198:1220	most pronounced changes in gene expression	1198:1239	Adult insulin sensitivity and dyslipidaemia were improved and most pronounced changes in gene expression were observed in the ileum.
29460474	10	9	theme	increased	1377:1385	arg1	abundance					1387:1395	increased abundance	1377:1395	increased abundance of Bifidobacterium	1377:1414	We observed subtle changes in faecal microbiota composition, both in early life and in adulthood, including increased abundance of Bifidobacterium.
29460474	5	10	theme	Western-style	704:716	arg1	WSD					724:726	WSD	724:726	WSD	724:726	Mice were subsequently challenged with a high-fat Western-style diet (WSD) for 8 weeks.
29460474	5	10	theme	Western-style	704:716	arg1	diet					718:721	a high-fat Western-style diet	693:721	a high-fat Western-style diet (WSD) for 8 weeks	693:739	Mice were subsequently challenged with a high-fat Western-style diet (WSD) for 8 weeks.
29460474	4	11	with	scGOS/lcFOS	514:524	arg1	M-16 V					553:558	Bifidobacterium breve M-16 V	531:558	Bifidobacterium breve M-16 V	531:558	METHODS Postnatal diets were supplemented with either prebiotics (scGOS/lcFOS) or synbiotics (scGOS/lcFOS with Bifidobacterium breve M-16 V) until post-natal (PN) day 42 in a well-established rodent model for nutritional programming.
29460474	11	12	theme	synbiotics-supplemented	1473:1495	arg1	mice					1508:1511	synbiotics-supplemented adolescent mice	1473:1511	synbiotics-supplemented adolescent mice	1473:1511	Microbiota transplantation using samples collected from synbiotics-supplemented adolescent mice at PN42 to age-matched germ-free recipients did not transfer the beneficial phenotype, indicating that synbiotics-modified microbiota at PN42 is not sufficient to transfer long-lasting protection of metabolic health status.
29460474	12	13	from	potential	1782:1790	arg1	life					1853:1856	early life	1847:1856	early life	1847:1856	CONCLUSION Together, these findings show the potential and importance of timing of synbiotic interventions in early life during crucial microbiota development as a preventive measure to lower the risk of obesity and improve metabolic health throughout life.
29460474	4	14	theme	nutritional	629:639	arg1	programming					641:651	nutritional programming	629:651	nutritional programming	629:651	METHODS Postnatal diets were supplemented with either prebiotics (scGOS/lcFOS) or synbiotics (scGOS/lcFOS with Bifidobacterium breve M-16 V) until post-natal (PN) day 42 in a well-established rodent model for nutritional programming.
29460474	11	15	from	PN42	1650:1653	arg1	microbiota					1636:1645	synbiotics-modified microbiota	1616:1645	synbiotics-modified microbiota at PN42	1616:1653	Microbiota transplantation using samples collected from synbiotics-supplemented adolescent mice at PN42 to age-matched germ-free recipients did not transfer the beneficial phenotype, indicating that synbiotics-modified microbiota at PN42 is not sufficient to transfer long-lasting protection of metabolic health status.
29460474	0	16	from	obesity	63:69	arg1	mice					80:83	adult mice	74:83	adult mice	74:83	Specific synbiotics in early life protect against diet-induced obesity in adult mice.
29460474	11	17	theme	beneficial	1578:1587	arg1	phenotype					1589:1597	the beneficial phenotype	1574:1597	the beneficial phenotype	1574:1597	Microbiota transplantation using samples collected from synbiotics-supplemented adolescent mice at PN42 to age-matched germ-free recipients did not transfer the beneficial phenotype, indicating that synbiotics-modified microbiota at PN42 is not sufficient to transfer long-lasting protection of metabolic health status.
29460474	10	18	theme	faecal	1299:1304	arg1	composition					1317:1327	faecal microbiota composition	1299:1327	faecal microbiota composition	1299:1327	We observed subtle changes in faecal microbiota composition, both in early life and in adulthood, including increased abundance of Bifidobacterium.
29460474	0	19	theme	adult	74:78	arg1	mice					80:83	adult mice	74:83	adult mice	74:83	Specific synbiotics in early life protect against diet-induced obesity in adult mice.
29460474	8	20	theme	Early	979:983	arg1	synbiotics					990:999	Early life synbiotics	979:999	Early life synbiotics	979:999	RESULTS Early life synbiotics protected mice against WSD-induced excessive fat accumulation throughout life, replicable in 2 independent European animal facilities.
29460474	7	21	theme	tissues	926:932	arg1	transcriptomics					898:912	host transcriptomics	893:912	host transcriptomics	893:912	Markers of glucose homeostasis, lipid metabolism and host transcriptomics of 6 target tissues were determined in adulthood (PN98).
29460474	7	21	theme	tissues	926:932	arg1	homeostasis					859:869	glucose homeostasis	851:869	glucose homeostasis	851:869	Markers of glucose homeostasis, lipid metabolism and host transcriptomics of 6 target tissues were determined in adulthood (PN98).
29460474	7	21	theme	tissues	926:932	arg1	metabolism					878:887	lipid metabolism	872:887	lipid metabolism	872:887	Markers of glucose homeostasis, lipid metabolism and host transcriptomics of 6 target tissues were determined in adulthood (PN98).
29460474	3	22	theme	beneficial	332:341	arg1	interventions					354:366	beneficial microbiome interventions	332:366	beneficial microbiome interventions in early life	332:380	Here, we determined how beneficial microbiome interventions in early life affect metabolic health in adulthood.
29460474	11	23	theme	germ-free	1536:1544	arg1	recipients					1546:1555	age-matched germ-free recipients	1524:1555	age-matched germ-free recipients	1524:1555	Microbiota transplantation using samples collected from synbiotics-supplemented adolescent mice at PN42 to age-matched germ-free recipients did not transfer the beneficial phenotype, indicating that synbiotics-modified microbiota at PN42 is not sufficient to transfer long-lasting protection of metabolic health status.
29460474	3	24	from	interventions	354:366	arg1	life					377:380	early life	371:380	early life	371:380	Here, we determined how beneficial microbiome interventions in early life affect metabolic health in adulthood.
29460474	1	25	theme	adults	120:125	arg1	state					105:109	The metabolic state	91:109	The metabolic state of human adults	91:125	AIMS The metabolic state of human adults is associated with their gut microbiome.
29460474	7	26	theme	transcriptomics	898:912	arg1	Markers					840:846	Markers	840:846	Markers of glucose homeostasis, lipid metabolism and host transcriptomics of 6 target tissues	840:932	Markers of glucose homeostasis, lipid metabolism and host transcriptomics of 6 target tissues were determined in adulthood (PN98).
29460474	3	27	theme	early	371:375	arg1	life					377:380	early life	371:380	early life	371:380	Here, we determined how beneficial microbiome interventions in early life affect metabolic health in adulthood.
29460474	0	28	theme	Specific	0:7	arg1	synbiotics					9:18	Specific synbiotics	0:18	Specific synbiotics in early life	0:32	Specific synbiotics in early life protect against diet-induced obesity in adult mice.
29460474	8	29	theme	WSD-induced	1024:1034	arg1	accumulation					1050:1061	WSD-induced excessive fat accumulation	1024:1061	WSD-induced excessive fat accumulation throughout life	1024:1077	RESULTS Early life synbiotics protected mice against WSD-induced excessive fat accumulation throughout life, replicable in 2 independent European animal facilities.
29460474	11	30	theme	adolescent	1497:1506	arg1	mice					1508:1511	synbiotics-supplemented adolescent mice	1473:1511	synbiotics-supplemented adolescent mice	1473:1511	Microbiota transplantation using samples collected from synbiotics-supplemented adolescent mice at PN42 to age-matched germ-free recipients did not transfer the beneficial phenotype, indicating that synbiotics-modified microbiota at PN42 is not sufficient to transfer long-lasting protection of metabolic health status.
29460474	8	31	theme	fat	1046:1048	arg1	accumulation					1050:1061	WSD-induced excessive fat accumulation	1024:1061	WSD-induced excessive fat accumulation throughout life	1024:1077	RESULTS Early life synbiotics protected mice against WSD-induced excessive fat accumulation throughout life, replicable in 2 independent European animal facilities.
29460474	8	32	from	facilities	1124:1133	arg1	replicable					1080:1089	replicable	1080:1089	replicable	1080:1089	RESULTS Early life synbiotics protected mice against WSD-induced excessive fat accumulation throughout life, replicable in 2 independent European animal facilities.
29460474	1	33	theme	metabolic	95:103	arg1	state					105:109	The metabolic state	91:109	The metabolic state of human adults	91:125	AIMS The metabolic state of human adults is associated with their gut microbiome.
29460474	6	34	theme	Body	742:745	arg1	composition					758:768	composition	758:768	composition	758:768	Body weight and composition were monitored, as was gut microbiota composition at PN21, 42 and 98.
29460474	6	34	theme	Body	742:745	arg1	composition					808:818	was gut microbiota composition	789:818	was gut microbiota composition at PN21, 42 and 98	789:837	Body weight and composition were monitored, as was gut microbiota composition at PN21, 42 and 98.
29460474	6	34	theme	Body	742:745	arg1	weight					747:752	Body weight	742:752	Body weight	742:752	Body weight and composition were monitored, as was gut microbiota composition at PN21, 42 and 98.
29460474	12	35	theme	preventive	1901:1910	arg1	importance					1796:1805	importance	1796:1805	importance	1796:1805	CONCLUSION Together, these findings show the potential and importance of timing of synbiotic interventions in early life during crucial microbiota development as a preventive measure to lower the risk of obesity and improve metabolic health throughout life.
29460474	12	35	theme	preventive	1901:1910	arg1	measure					1912:1918	a preventive measure	1899:1918	a preventive measure to lower the risk of obesity and improve metabolic health throughout life	1899:1992	CONCLUSION Together, these findings show the potential and importance of timing of synbiotic interventions in early life during crucial microbiota development as a preventive measure to lower the risk of obesity and improve metabolic health throughout life.
29460474	12	35	theme	preventive	1901:1910	arg1	potential					1782:1790	potential	1782:1790	potential	1782:1790	CONCLUSION Together, these findings show the potential and importance of timing of synbiotic interventions in early life during crucial microbiota development as a preventive measure to lower the risk of obesity and improve metabolic health throughout life.
29460474	4	36	theme	Bifidobacterium	531:545	arg1	M-16 V					553:558	Bifidobacterium breve M-16 V	531:558	Bifidobacterium breve M-16 V	531:558	METHODS Postnatal diets were supplemented with either prebiotics (scGOS/lcFOS) or synbiotics (scGOS/lcFOS with Bifidobacterium breve M-16 V) until post-natal (PN) day 42 in a well-established rodent model for nutritional programming.
29460474	7	37	theme	metabolism	878:887	arg1	Markers					840:846	Markers	840:846	Markers of glucose homeostasis, lipid metabolism and host transcriptomics of 6 target tissues	840:932	Markers of glucose homeostasis, lipid metabolism and host transcriptomics of 6 target tissues were determined in adulthood (PN98).
29460474	9	38	theme	Adult	1136:1140	arg1	sensitivity					1150:1160	Adult insulin sensitivity	1136:1160	Adult insulin sensitivity	1136:1160	Adult insulin sensitivity and dyslipidaemia were improved and most pronounced changes in gene expression were observed in the ileum.
29460474	8	39	theme	independent	1096:1106	arg1	facilities					1124:1133	2 independent European animal facilities	1094:1133	2 independent European animal facilities	1094:1133	RESULTS Early life synbiotics protected mice against WSD-induced excessive fat accumulation throughout life, replicable in 2 independent European animal facilities.
29460474	12	40	theme	crucial	1865:1871	arg1	development					1884:1894	crucial microbiota development	1865:1894	crucial microbiota development	1865:1894	CONCLUSION Together, these findings show the potential and importance of timing of synbiotic interventions in early life during crucial microbiota development as a preventive measure to lower the risk of obesity and improve metabolic health throughout life.
29460474	10	41	theme	early	1338:1342	arg1	life					1344:1347	early life	1338:1347	early life	1338:1347	We observed subtle changes in faecal microbiota composition, both in early life and in adulthood, including increased abundance of Bifidobacterium.
29460474	7	42	theme	glucose	851:857	arg1	homeostasis					859:869	glucose homeostasis	851:869	glucose homeostasis	851:869	Markers of glucose homeostasis, lipid metabolism and host transcriptomics of 6 target tissues were determined in adulthood (PN98).
29460474	8	43	theme	animal	1117:1122	arg1	facilities					1124:1133	2 independent European animal facilities	1094:1133	2 independent European animal facilities	1094:1133	RESULTS Early life synbiotics protected mice against WSD-induced excessive fat accumulation throughout life, replicable in 2 independent European animal facilities.
29460474	12	44	theme	synbiotic	1820:1828	arg1	interventions					1830:1842	synbiotic interventions	1820:1842	synbiotic interventions	1820:1842	CONCLUSION Together, these findings show the potential and importance of timing of synbiotic interventions in early life during crucial microbiota development as a preventive measure to lower the risk of obesity and improve metabolic health throughout life.
29460474	11	45	theme	long-lasting	1685:1696	arg1	protection					1698:1707	long-lasting protection	1685:1707	long-lasting protection of metabolic health status	1685:1734	Microbiota transplantation using samples collected from synbiotics-supplemented adolescent mice at PN42 to age-matched germ-free recipients did not transfer the beneficial phenotype, indicating that synbiotics-modified microbiota at PN42 is not sufficient to transfer long-lasting protection of metabolic health status.
29460474	6	46	theme	was	789:791	arg1	composition					758:768	composition	758:768	composition	758:768	Body weight and composition were monitored, as was gut microbiota composition at PN21, 42 and 98.
29460474	6	46	theme	was	789:791	arg1	composition					808:818	was gut microbiota composition	789:818	was gut microbiota composition at PN21, 42 and 98	789:837	Body weight and composition were monitored, as was gut microbiota composition at PN21, 42 and 98.
29460474	6	46	theme	was	789:791	arg1	weight					747:752	Body weight	742:752	Body weight	742:752	Body weight and composition were monitored, as was gut microbiota composition at PN21, 42 and 98.
29460474	12	47	dep	risk	1933:1936	arg1	lower					1923:1927	lower	1923:1927	lower	1923:1927	CONCLUSION Together, these findings show the potential and importance of timing of synbiotic interventions in early life during crucial microbiota development as a preventive measure to lower the risk of obesity and improve metabolic health throughout life.
29460474	12	48	dep	CONCLUSION	1737:1746	arg1	show					1773:1776	show	1773:1776	show the potential and importance of timing of synbiotic interventions in early life during crucial microbiota development as a preventive measure to lower the risk of obesity and improve metabolic health throughout life	1773:1992	CONCLUSION Together, these findings show the potential and importance of timing of synbiotic interventions in early life during crucial microbiota development as a preventive measure to lower the risk of obesity and improve metabolic health throughout life.
29460474	2	49	theme	microbiome	248:257	arg1	perturbation					259:270	early life microbiome perturbation	237:270	early life microbiome perturbation	237:270	The symbiosis between host and microbiome is initiated at birth, and early life microbiome perturbation can disturb health throughout life.
29460474	1	50	theme	gut	152:154	arg1	microbiome					156:165	their gut microbiome	146:165	their gut microbiome	146:165	AIMS The metabolic state of human adults is associated with their gut microbiome.
29460474	6	51	from	42	829:830	arg1	composition					758:768	composition	758:768	composition	758:768	Body weight and composition were monitored, as was gut microbiota composition at PN21, 42 and 98.
29460474	6	51	from	42	829:830	arg1	composition					808:818	was gut microbiota composition	789:818	was gut microbiota composition at PN21, 42 and 98	789:837	Body weight and composition were monitored, as was gut microbiota composition at PN21, 42 and 98.
29460474	6	51	from	42	829:830	arg1	weight					747:752	Body weight	742:752	Body weight	742:752	Body weight and composition were monitored, as was gut microbiota composition at PN21, 42 and 98.
29460474	11	52	theme	health	1722:1727	arg1	status					1729:1734	metabolic health status	1712:1734	metabolic health status	1712:1734	Microbiota transplantation using samples collected from synbiotics-supplemented adolescent mice at PN42 to age-matched germ-free recipients did not transfer the beneficial phenotype, indicating that synbiotics-modified microbiota at PN42 is not sufficient to transfer long-lasting protection of metabolic health status.
29460474	10	53	theme	Bifidobacterium	1400:1414	arg1	abundance					1387:1395	increased abundance	1377:1395	increased abundance of Bifidobacterium	1377:1414	We observed subtle changes in faecal microbiota composition, both in early life and in adulthood, including increased abundance of Bifidobacterium.
29460474	12	54	theme	timing	1810:1815	arg1	measure					1912:1918	a preventive measure	1899:1918	a preventive measure to lower the risk of obesity and improve metabolic health throughout life	1899:1992	CONCLUSION Together, these findings show the potential and importance of timing of synbiotic interventions in early life during crucial microbiota development as a preventive measure to lower the risk of obesity and improve metabolic health throughout life.
29460474	12	54	theme	timing	1810:1815	arg1	importance					1796:1805	importance	1796:1805	importance	1796:1805	CONCLUSION Together, these findings show the potential and importance of timing of synbiotic interventions in early life during crucial microbiota development as a preventive measure to lower the risk of obesity and improve metabolic health throughout life.
29460474	12	54	theme	timing	1810:1815	arg1	potential					1782:1790	potential	1782:1790	potential	1782:1790	CONCLUSION Together, these findings show the potential and importance of timing of synbiotic interventions in early life during crucial microbiota development as a preventive measure to lower the risk of obesity and improve metabolic health throughout life.
29460474	6	55	from	98	836:837	arg1	composition					758:768	composition	758:768	composition	758:768	Body weight and composition were monitored, as was gut microbiota composition at PN21, 42 and 98.
29460474	6	55	from	98	836:837	arg1	composition					808:818	was gut microbiota composition	789:818	was gut microbiota composition at PN21, 42 and 98	789:837	Body weight and composition were monitored, as was gut microbiota composition at PN21, 42 and 98.
29460474	6	55	from	98	836:837	arg1	weight					747:752	Body weight	742:752	Body weight	742:752	Body weight and composition were monitored, as was gut microbiota composition at PN21, 42 and 98.
29460474	5	56	theme	high-fat	695:702	arg1	WSD					724:726	WSD	724:726	WSD	724:726	Mice were subsequently challenged with a high-fat Western-style diet (WSD) for 8 weeks.
29460474	5	56	theme	high-fat	695:702	arg1	diet					718:721	a high-fat Western-style diet	693:721	a high-fat Western-style diet (WSD) for 8 weeks	693:739	Mice were subsequently challenged with a high-fat Western-style diet (WSD) for 8 weeks.
29460474	4	57	theme	rodent	612:617	arg1	model					619:623	a well-established rodent model	593:623	a well-established rodent model for nutritional programming	593:651	METHODS Postnatal diets were supplemented with either prebiotics (scGOS/lcFOS) or synbiotics (scGOS/lcFOS with Bifidobacterium breve M-16 V) until post-natal (PN) day 42 in a well-established rodent model for nutritional programming.
29460474	9	58	theme	gene	1225:1228	arg1	expression					1230:1239	gene expression	1225:1239	gene expression	1225:1239	Adult insulin sensitivity and dyslipidaemia were improved and most pronounced changes in gene expression were observed in the ileum.
29460474	6	59	from	PN21	823:826	arg1	composition					758:768	composition	758:768	composition	758:768	Body weight and composition were monitored, as was gut microbiota composition at PN21, 42 and 98.
29460474	6	59	from	PN21	823:826	arg1	composition					808:818	was gut microbiota composition	789:818	was gut microbiota composition at PN21, 42 and 98	789:837	Body weight and composition were monitored, as was gut microbiota composition at PN21, 42 and 98.
29460474	6	59	from	PN21	823:826	arg1	weight					747:752	Body weight	742:752	Body weight	742:752	Body weight and composition were monitored, as was gut microbiota composition at PN21, 42 and 98.
29460474	9	60	from	changes	1214:1220	arg1	expression					1230:1239	gene expression	1225:1239	gene expression	1225:1239	Adult insulin sensitivity and dyslipidaemia were improved and most pronounced changes in gene expression were observed in the ileum.
29460474	2	61	theme	early	237:241	arg1	perturbation					259:270	early life microbiome perturbation	237:270	early life microbiome perturbation	237:270	The symbiosis between host and microbiome is initiated at birth, and early life microbiome perturbation can disturb health throughout life.
29460474	1	62	theme	human	114:118	arg1	adults					120:125	human adults	114:125	human adults	114:125	AIMS The metabolic state of human adults is associated with their gut microbiome.
29460474	4	63	theme	Postnatal	428:436	arg1	diets					438:442	Postnatal diets	428:442	Postnatal diets	428:442	METHODS Postnatal diets were supplemented with either prebiotics (scGOS/lcFOS) or synbiotics (scGOS/lcFOS with Bifidobacterium breve M-16 V) until post-natal (PN) day 42 in a well-established rodent model for nutritional programming.
29460474	10	64	from	changes	1288:1294	arg1	life					1344:1347	early life	1338:1347	early life	1338:1347	We observed subtle changes in faecal microbiota composition, both in early life and in adulthood, including increased abundance of Bifidobacterium.
29460474	10	64	from	changes	1288:1294	arg1	adulthood					1356:1364	adulthood	1356:1364	adulthood	1356:1364	We observed subtle changes in faecal microbiota composition, both in early life and in adulthood, including increased abundance of Bifidobacterium.
29460474	10	64	from	changes	1288:1294	arg1	composition					1317:1327	faecal microbiota composition	1299:1327	faecal microbiota composition	1299:1327	We observed subtle changes in faecal microbiota composition, both in early life and in adulthood, including increased abundance of Bifidobacterium.
29460474	8	65	theme	life	985:988	arg1	synbiotics					990:999	Early life synbiotics	979:999	Early life synbiotics	979:999	RESULTS Early life synbiotics protected mice against WSD-induced excessive fat accumulation throughout life, replicable in 2 independent European animal facilities.
29460474	0	66	from	synbiotics	9:18	arg1	life					29:32	early life	23:32	early life	23:32	Specific synbiotics in early life protect against diet-induced obesity in adult mice.
29460474	11	67	theme	age-matched	1524:1534	arg1	recipients					1546:1555	age-matched germ-free recipients	1524:1555	age-matched germ-free recipients	1524:1555	Microbiota transplantation using samples collected from synbiotics-supplemented adolescent mice at PN42 to age-matched germ-free recipients did not transfer the beneficial phenotype, indicating that synbiotics-modified microbiota at PN42 is not sufficient to transfer long-lasting protection of metabolic health status.
29460474	4	68	from	day	583:585	arg1	model					619:623	a well-established rodent model	593:623	a well-established rodent model for nutritional programming	593:651	METHODS Postnatal diets were supplemented with either prebiotics (scGOS/lcFOS) or synbiotics (scGOS/lcFOS with Bifidobacterium breve M-16 V) until post-natal (PN) day 42 in a well-established rodent model for nutritional programming.
29460474	8	69	from	replicable	1080:1089	arg1	facilities					1124:1133	2 independent European animal facilities	1094:1133	2 independent European animal facilities	1094:1133	RESULTS Early life synbiotics protected mice against WSD-induced excessive fat accumulation throughout life, replicable in 2 independent European animal facilities.
29460474	3	70	theme	microbiome	343:352	arg1	interventions					354:366	beneficial microbiome interventions	332:366	beneficial microbiome interventions in early life	332:380	Here, we determined how beneficial microbiome interventions in early life affect metabolic health in adulthood.
29460474	8	71	dep	RESULTS	971:977	arg1	protected					1001:1009	protected	1001:1009	protected	1001:1009	RESULTS Early life synbiotics protected mice against WSD-induced excessive fat accumulation throughout life, replicable in 2 independent European animal facilities.
29460474	10	72	theme	subtle	1281:1286	arg1	abundance					1387:1395	increased abundance	1377:1395	increased abundance of Bifidobacterium	1377:1414	We observed subtle changes in faecal microbiota composition, both in early life and in adulthood, including increased abundance of Bifidobacterium.
29460474	10	72	theme	subtle	1281:1286	arg1	changes					1288:1294	subtle changes	1281:1294	subtle changes	1281:1294	We observed subtle changes in faecal microbiota composition, both in early life and in adulthood, including increased abundance of Bifidobacterium.
29460474	7	73	theme	target	919:924	arg1	tissues					926:932	6 target tissues	917:932	6 target tissues	917:932	Markers of glucose homeostasis, lipid metabolism and host transcriptomics of 6 target tissues were determined in adulthood (PN98).
29460474	11	74	theme	Microbiota	1417:1426	arg1	transplantation					1428:1442	Microbiota transplantation	1417:1442	Microbiota transplantation using samples collected from synbiotics-supplemented adolescent mice at PN42 to age-matched germ-free recipients	1417:1555	Microbiota transplantation using samples collected from synbiotics-supplemented adolescent mice at PN42 to age-matched germ-free recipients did not transfer the beneficial phenotype, indicating that synbiotics-modified microbiota at PN42 is not sufficient to transfer long-lasting protection of metabolic health status.
29460474	8	75	theme	excessive	1036:1044	arg1	accumulation					1050:1061	WSD-induced excessive fat accumulation	1024:1061	WSD-induced excessive fat accumulation throughout life	1024:1077	RESULTS Early life synbiotics protected mice against WSD-induced excessive fat accumulation throughout life, replicable in 2 independent European animal facilities.
29460474	10	76	theme	microbiota	1306:1315	arg1	composition					1317:1327	faecal microbiota composition	1299:1327	faecal microbiota composition	1299:1327	We observed subtle changes in faecal microbiota composition, both in early life and in adulthood, including increased abundance of Bifidobacterium.
29460474	0	77	theme	early	23:27	arg1	life					29:32	early life	23:32	early life	23:32	Specific synbiotics in early life protect against diet-induced obesity in adult mice.
29460474	7	78	theme	host	893:896	arg1	transcriptomics					898:912	host transcriptomics	893:912	host transcriptomics	893:912	Markers of glucose homeostasis, lipid metabolism and host transcriptomics of 6 target tissues were determined in adulthood (PN98).
29460474	4	79	theme	post-natal	567:576	arg1	day					583:585	post-natal (PN) day 42	567:588	post-natal (PN) day 42 in a well-established rodent model for nutritional programming	567:651	METHODS Postnatal diets were supplemented with either prebiotics (scGOS/lcFOS) or synbiotics (scGOS/lcFOS with Bifidobacterium breve M-16 V) until post-natal (PN) day 42 in a well-established rodent model for nutritional programming.
29460474	4	79	theme	post-natal	567:576	arg1	PN					579:580	PN	579:580	PN	579:580	METHODS Postnatal diets were supplemented with either prebiotics (scGOS/lcFOS) or synbiotics (scGOS/lcFOS with Bifidobacterium breve M-16 V) until post-natal (PN) day 42 in a well-established rodent model for nutritional programming.
29460474	12	80	theme	obesity	1941:1947	arg1	risk					1933:1936	the risk	1929:1936	the risk of obesity	1929:1947	CONCLUSION Together, these findings show the potential and importance of timing of synbiotic interventions in early life during crucial microbiota development as a preventive measure to lower the risk of obesity and improve metabolic health throughout life.
29460474	12	81	from	importance	1796:1805	arg1	life					1853:1856	early life	1847:1856	early life	1847:1856	CONCLUSION Together, these findings show the potential and importance of timing of synbiotic interventions in early life during crucial microbiota development as a preventive measure to lower the risk of obesity and improve metabolic health throughout life.
29460474	0	82	theme	diet-induced	50:61	arg1	obesity					63:69	diet-induced obesity	50:69	diet-induced obesity in adult mice	50:83	Specific synbiotics in early life protect against diet-induced obesity in adult mice.
29460474	4	83	dep	synbiotics	502:511	arg1	scGOS/lcFOS					514:524	scGOS/lcFOS	514:524	scGOS/lcFOS with Bifidobacterium breve M-16 V	514:558	METHODS Postnatal diets were supplemented with either prebiotics (scGOS/lcFOS) or synbiotics (scGOS/lcFOS with Bifidobacterium breve M-16 V) until post-natal (PN) day 42 in a well-established rodent model for nutritional programming.
29460474	12	84	theme	microbiota	1873:1882	arg1	development					1884:1894	crucial microbiota development	1865:1894	crucial microbiota development	1865:1894	CONCLUSION Together, these findings show the potential and importance of timing of synbiotic interventions in early life during crucial microbiota development as a preventive measure to lower the risk of obesity and improve metabolic health throughout life.
29460474	3	85	theme	metabolic	389:397	arg1	health					399:404	metabolic health	389:404	metabolic health	389:404	Here, we determined how beneficial microbiome interventions in early life affect metabolic health in adulthood.
29460474	4	86	theme	breve	547:551	arg1	M-16 V					553:558	Bifidobacterium breve M-16 V	531:558	Bifidobacterium breve M-16 V	531:558	METHODS Postnatal diets were supplemented with either prebiotics (scGOS/lcFOS) or synbiotics (scGOS/lcFOS with Bifidobacterium breve M-16 V) until post-natal (PN) day 42 in a well-established rodent model for nutritional programming.
29460474	11	87	theme	synbiotics-modified	1616:1634	arg1	microbiota					1636:1645	synbiotics-modified microbiota	1616:1645	synbiotics-modified microbiota at PN42	1616:1653	Microbiota transplantation using samples collected from synbiotics-supplemented adolescent mice at PN42 to age-matched germ-free recipients did not transfer the beneficial phenotype, indicating that synbiotics-modified microbiota at PN42 is not sufficient to transfer long-lasting protection of metabolic health status.
29460474	7	88	theme	lipid	872:876	arg1	metabolism					878:887	lipid metabolism	872:887	lipid metabolism	872:887	Markers of glucose homeostasis, lipid metabolism and host transcriptomics of 6 target tissues were determined in adulthood (PN98).
29460474	7	89	theme	homeostasis	859:869	arg1	Markers					840:846	Markers	840:846	Markers of glucose homeostasis, lipid metabolism and host transcriptomics of 6 target tissues	840:932	Markers of glucose homeostasis, lipid metabolism and host transcriptomics of 6 target tissues were determined in adulthood (PN98).
29460474	4	90	dep	METHODS	420:426	arg1	supplemented					449:460	supplemented	449:460	were supplemented with either prebiotics (scGOS/lcFOS) or synbiotics (scGOS/lcFOS with Bifidobacterium breve M-16 V) until post-natal (PN) day 42 in a well-established rodent model for nutritional programming	444:651	METHODS Postnatal diets were supplemented with either prebiotics (scGOS/lcFOS) or synbiotics (scGOS/lcFOS with Bifidobacterium breve M-16 V) until post-natal (PN) day 42 in a well-established rodent model for nutritional programming.
29460474	8	91	theme	European	1108:1115	arg1	facilities					1124:1133	2 independent European animal facilities	1094:1133	2 independent European animal facilities	1094:1133	RESULTS Early life synbiotics protected mice against WSD-induced excessive fat accumulation throughout life, replicable in 2 independent European animal facilities.
29460474	6	92	theme	microbiota	797:806	arg1	composition					758:768	composition	758:768	composition	758:768	Body weight and composition were monitored, as was gut microbiota composition at PN21, 42 and 98.
29460474	6	92	theme	microbiota	797:806	arg1	composition					808:818	was gut microbiota composition	789:818	was gut microbiota composition at PN21, 42 and 98	789:837	Body weight and composition were monitored, as was gut microbiota composition at PN21, 42 and 98.
29460474	6	92	theme	microbiota	797:806	arg1	weight					747:752	Body weight	742:752	Body weight	742:752	Body weight and composition were monitored, as was gut microbiota composition at PN21, 42 and 98.
29460474	12	93	theme	early	1847:1851	arg1	life					1853:1856	early life	1847:1856	early life	1847:1856	CONCLUSION Together, these findings show the potential and importance of timing of synbiotic interventions in early life during crucial microbiota development as a preventive measure to lower the risk of obesity and improve metabolic health throughout life.
29460474	4	94	theme	well-established	595:610	arg1	model					619:623	a well-established rodent model	593:623	a well-established rodent model for nutritional programming	593:651	METHODS Postnatal diets were supplemented with either prebiotics (scGOS/lcFOS) or synbiotics (scGOS/lcFOS with Bifidobacterium breve M-16 V) until post-natal (PN) day 42 in a well-established rodent model for nutritional programming.
29460474	9	95	theme	insulin	1142:1148	arg1	sensitivity					1150:1160	Adult insulin sensitivity	1136:1160	Adult insulin sensitivity	1136:1160	Adult insulin sensitivity and dyslipidaemia were improved and most pronounced changes in gene expression were observed in the ileum.
30407556	0	0	theme	Arabidopsis	94:104	arg1	thaliana					106:113	Arabidopsis thaliana	94:113	Arabidopsis thaliana	94:113	Microtubule-associated protein IQ67 DOMAIN5 regulates morphogenesis of leaf pavement cells in Arabidopsis thaliana.
30407556	8	1	theme	calmodulin	1212:1221	arg1	sensors					1231:1237	calmodulin calcium sensors	1212:1237	calmodulin calcium sensors	1212:1237	Lastly, we demonstrate IQD5-dependent recruitment of calmodulin calcium sensors to cortical microtubule arrays and provide first evidence for important roles for calcium in regulation of PC morphogenesis.
30407556	3	2	with	MAPs	543:546	arg1	functions					569:577	largely unknown functions	553:577	largely unknown functions	553:577	IQ67 DOMAIN (IQD) proteins recently emerged as a class of plant-specific MAPs with largely unknown functions.
30407556	9	3	theme	novel	1394:1398	arg1	player					1400:1405	a novel player	1392:1405	a novel player in PC shape regulation	1392:1428	Our work identifies IQD5 as a novel player in PC shape regulation and, for the first time, links calcium signaling to developmental processes that regulate anisotropic growth in PCs.
30407556	9	3	theme	novel	1394:1398	arg1	IQD5					1384:1387	IQD5	1384:1387	IQD5	1384:1387	Our work identifies IQD5 as a novel player in PC shape regulation and, for the first time, links calcium signaling to developmental processes that regulate anisotropic growth in PCs.
30407556	3	4	theme	MAPs	543:546	arg1	class					519:523	a class	517:523	a class of plant-specific MAPs with largely unknown functions	517:577	IQ67 DOMAIN (IQD) proteins recently emerged as a class of plant-specific MAPs with largely unknown functions.
30407556	9	5	from	player	1400:1405	arg1	regulation					1419:1428	PC shape regulation	1410:1428	PC shape regulation	1410:1428	Our work identifies IQD5 as a novel player in PC shape regulation and, for the first time, links calcium signaling to developmental processes that regulate anisotropic growth in PCs.
30407556	7	6	theme	cell	1004:1007	arg1	composition					1014:1024	cell wall composition	1004:1024	cell wall composition	1004:1024	Histochemical analysis of cell wall composition further suggests reduced rates of cellulose deposition in anticlinal cell walls, which correlate with reduced anisotropic expansion.
30407556	1	7	theme	important	184:192	arg1	roles					194:198	important roles	184:198	important roles for regulating cell division, cell proliferation, and cell morphology	184:268	Plant microtubules form a highly dynamic intracellular network with important roles for regulating cell division, cell proliferation, and cell morphology.
30407556	9	8	theme	calcium	1461:1467	arg1	signaling					1469:1477	calcium signaling	1461:1477	calcium signaling	1461:1477	Our work identifies IQD5 as a novel player in PC shape regulation and, for the first time, links calcium signaling to developmental processes that regulate anisotropic growth in PCs.
30407556	8	9	theme	sensors	1231:1237	arg1	recruitment					1197:1207	IQD5-dependent recruitment	1182:1207	IQD5-dependent recruitment of calmodulin calcium sensors to cortical microtubule arrays	1182:1268	Lastly, we demonstrate IQD5-dependent recruitment of calmodulin calcium sensors to cortical microtubule arrays and provide first evidence for important roles for calcium in regulation of PC morphogenesis.
30407556	6	10	theme	cell	947:950	arg1	morphogenesis					963:975	pavement cell (PC) shape morphogenesis	938:975	pavement cell (PC) shape morphogenesis	938:975	Our phenotypic analysis of iqd5 loss-of-function lines reveals functions of IQD5 in pavement cell (PC) shape morphogenesis.
30407556	2	11	theme	environmental	385:397	arg1	stimuli					417:423	environmental and developmental stimuli	385:423	environmental and developmental stimuli to fine-tune	385:436	Their organization and dynamics are co-ordinated by various microtubule-associated proteins (MAPs) that integrate environmental and developmental stimuli to fine-tune and adjust cytoskeletal arrays.
30407556	7	12	theme	wall	1009:1012	arg1	composition					1014:1024	cell wall composition	1004:1024	cell wall composition	1004:1024	Histochemical analysis of cell wall composition further suggests reduced rates of cellulose deposition in anticlinal cell walls, which correlate with reduced anisotropic expansion.
30407556	8	13	theme	calcium	1223:1229	arg1	sensors					1231:1237	calmodulin calcium sensors	1212:1237	calmodulin calcium sensors	1212:1237	Lastly, we demonstrate IQD5-dependent recruitment of calmodulin calcium sensors to cortical microtubule arrays and provide first evidence for important roles for calcium in regulation of PC morphogenesis.
30407556	6	14	theme	pavement	938:945	arg1	PC					953:954	PC	953:954	PC	953:954	Our phenotypic analysis of iqd5 loss-of-function lines reveals functions of IQD5 in pavement cell (PC) shape morphogenesis.
30407556	6	14	theme	pavement	938:945	arg1	cell					947:950	pavement cell	938:950	pavement cell (PC) shape morphogenesis	938:975	Our phenotypic analysis of iqd5 loss-of-function lines reveals functions of IQD5 in pavement cell (PC) shape morphogenesis.
30407556	1	15	with	network	171:177	arg1	roles					194:198	important roles	184:198	important roles for regulating cell division, cell proliferation, and cell morphology	184:268	Plant microtubules form a highly dynamic intracellular network with important roles for regulating cell division, cell proliferation, and cell morphology.
30407556	9	16	theme	PC	1410:1411	arg1	regulation					1419:1428	PC shape regulation	1410:1428	PC shape regulation	1410:1428	Our work identifies IQD5 as a novel player in PC shape regulation and, for the first time, links calcium signaling to developmental processes that regulate anisotropic growth in PCs.
30407556	8	17	theme	IQD5-dependent	1182:1195	arg1	recruitment					1197:1207	IQD5-dependent recruitment	1182:1207	IQD5-dependent recruitment of calmodulin calcium sensors to cortical microtubule arrays	1182:1268	Lastly, we demonstrate IQD5-dependent recruitment of calmodulin calcium sensors to cortical microtubule arrays and provide first evidence for important roles for calcium in regulation of PC morphogenesis.
30407556	4	18	theme	functions	731:739	arg1	terms					657:661	terms	657:661	terms of its expression domains, subcellular localization, and biological functions	657:739	Here, using a reverse genetics approach, we characterize Arabidopsis IQD5 in terms of its expression domains, subcellular localization, and biological functions.
30407556	5	19	theme	microtubule	834:844	arg1	arrays					846:851	cortical microtubule arrays	825:851	cortical microtubule arrays	825:851	We show that IQD5 is expressed mostly in vegetative tissues, where it localizes to cortical microtubule arrays.
30407556	4	20	theme	subcellular	690:700	arg1	localization					702:713	subcellular localization	690:713	subcellular localization	690:713	Here, using a reverse genetics approach, we characterize Arabidopsis IQD5 in terms of its expression domains, subcellular localization, and biological functions.
30407556	9	21	theme	anisotropic	1520:1530	arg1	growth					1532:1537	anisotropic growth	1520:1537	anisotropic growth in PCs	1520:1544	Our work identifies IQD5 as a novel player in PC shape regulation and, for the first time, links calcium signaling to developmental processes that regulate anisotropic growth in PCs.
30407556	0	22	from	morphogenesis	54:66	arg1	thaliana					106:113	Arabidopsis thaliana	94:113	Arabidopsis thaliana	94:113	Microtubule-associated protein IQ67 DOMAIN5 regulates morphogenesis of leaf pavement cells in Arabidopsis thaliana.
30407556	8	23	theme	microtubule	1251:1261	arg1	arrays					1263:1268	cortical microtubule arrays	1242:1268	cortical microtubule arrays	1242:1268	Lastly, we demonstrate IQD5-dependent recruitment of calmodulin calcium sensors to cortical microtubule arrays and provide first evidence for important roles for calcium in regulation of PC morphogenesis.
30407556	7	24	theme	anisotropic	1136:1146	arg1	expansion					1148:1156	reduced anisotropic expansion	1128:1156	reduced anisotropic expansion	1128:1156	Histochemical analysis of cell wall composition further suggests reduced rates of cellulose deposition in anticlinal cell walls, which correlate with reduced anisotropic expansion.
30407556	8	25	theme	cortical	1242:1249	arg1	arrays					1263:1268	cortical microtubule arrays	1242:1268	cortical microtubule arrays	1242:1268	Lastly, we demonstrate IQD5-dependent recruitment of calmodulin calcium sensors to cortical microtubule arrays and provide first evidence for important roles for calcium in regulation of PC morphogenesis.
30407556	0	26	theme	protein	23:29	arg1	DOMAIN5					36:42	Microtubule-associated protein IQ67 DOMAIN5	0:42	Microtubule-associated protein IQ67 DOMAIN5	0:42	Microtubule-associated protein IQ67 DOMAIN5 regulates morphogenesis of leaf pavement cells in Arabidopsis thaliana.
30407556	3	27	theme	unknown	561:567	arg1	functions					569:577	largely unknown functions	553:577	largely unknown functions	553:577	IQ67 DOMAIN (IQD) proteins recently emerged as a class of plant-specific MAPs with largely unknown functions.
30407556	7	28	theme	reduced	1128:1134	arg1	expansion					1148:1156	reduced anisotropic expansion	1128:1156	reduced anisotropic expansion	1128:1156	Histochemical analysis of cell wall composition further suggests reduced rates of cellulose deposition in anticlinal cell walls, which correlate with reduced anisotropic expansion.
30407556	5	29	theme	vegetative	783:792	arg1	tissues					794:800	vegetative tissues	783:800	vegetative tissues	783:800	We show that IQD5 is expressed mostly in vegetative tissues, where it localizes to cortical microtubule arrays.
30407556	3	30	theme	DOMAIN	475:480	arg1	proteins					488:495	IQ67 DOMAIN (IQD) proteins	470:495	IQ67 DOMAIN (IQD) proteins	470:495	IQ67 DOMAIN (IQD) proteins recently emerged as a class of plant-specific MAPs with largely unknown functions.
30407556	0	31	theme	Microtubule-associated	0:21	arg1	DOMAIN5					36:42	Microtubule-associated protein IQ67 DOMAIN5	0:42	Microtubule-associated protein IQ67 DOMAIN5	0:42	Microtubule-associated protein IQ67 DOMAIN5 regulates morphogenesis of leaf pavement cells in Arabidopsis thaliana.
30407556	6	32	theme	phenotypic	858:867	arg1	analysis					869:876	Our phenotypic analysis	854:876	Our phenotypic analysis of iqd5 loss-of-function lines	854:907	Our phenotypic analysis of iqd5 loss-of-function lines reveals functions of IQD5 in pavement cell (PC) shape morphogenesis.
30407556	1	33	theme	cell	215:218	arg1	division					220:227	cell division	215:227	cell division	215:227	Plant microtubules form a highly dynamic intracellular network with important roles for regulating cell division, cell proliferation, and cell morphology.
30407556	6	34	from	functions	917:925	arg1	morphogenesis					963:975	pavement cell (PC) shape morphogenesis	938:975	pavement cell (PC) shape morphogenesis	938:975	Our phenotypic analysis of iqd5 loss-of-function lines reveals functions of IQD5 in pavement cell (PC) shape morphogenesis.
30407556	9	35	dep	identifies	1373:1382	arg1	links					1455:1459	links	1455:1459	links calcium signaling to developmental processes that regulate anisotropic growth in PCs	1455:1544	Our work identifies IQD5 as a novel player in PC shape regulation and, for the first time, links calcium signaling to developmental processes that regulate anisotropic growth in PCs.
30407556	9	36	theme	developmental	1482:1494	arg1	processes					1496:1504	developmental processes	1482:1504	developmental processes that regulate anisotropic growth in PCs	1482:1544	Our work identifies IQD5 as a novel player in PC shape regulation and, for the first time, links calcium signaling to developmental processes that regulate anisotropic growth in PCs.
30407556	2	37	theme	microtubule-associated	331:352	arg1	MAPs					364:367	MAPs	364:367	MAPs	364:367	Their organization and dynamics are co-ordinated by various microtubule-associated proteins (MAPs) that integrate environmental and developmental stimuli to fine-tune and adjust cytoskeletal arrays.
30407556	2	37	theme	microtubule-associated	331:352	arg1	proteins					354:361	various microtubule-associated proteins	323:361	various microtubule-associated proteins (MAPs) that integrate environmental and developmental stimuli to fine-tune and adjust cytoskeletal arrays	323:467	Their organization and dynamics are co-ordinated by various microtubule-associated proteins (MAPs) that integrate environmental and developmental stimuli to fine-tune and adjust cytoskeletal arrays.
30407556	7	38	theme	deposition	1070:1079	arg1	rates					1051:1055	reduced rates	1043:1055	reduced rates of cellulose deposition in anticlinal cell walls, which correlate with reduced anisotropic expansion	1043:1156	Histochemical analysis of cell wall composition further suggests reduced rates of cellulose deposition in anticlinal cell walls, which correlate with reduced anisotropic expansion.
30407556	0	39	theme	IQ67	31:34	arg1	DOMAIN5					36:42	Microtubule-associated protein IQ67 DOMAIN5	0:42	Microtubule-associated protein IQ67 DOMAIN5	0:42	Microtubule-associated protein IQ67 DOMAIN5 regulates morphogenesis of leaf pavement cells in Arabidopsis thaliana.
30407556	4	40	theme	genetics	602:609	arg1	approach					611:618	a reverse genetics approach	592:618	a reverse genetics approach	592:618	Here, using a reverse genetics approach, we characterize Arabidopsis IQD5 in terms of its expression domains, subcellular localization, and biological functions.
30407556	2	41	theme	various	323:329	arg1	MAPs					364:367	MAPs	364:367	MAPs	364:367	Their organization and dynamics are co-ordinated by various microtubule-associated proteins (MAPs) that integrate environmental and developmental stimuli to fine-tune and adjust cytoskeletal arrays.
30407556	2	41	theme	various	323:329	arg1	proteins					354:361	various microtubule-associated proteins	323:361	various microtubule-associated proteins (MAPs) that integrate environmental and developmental stimuli to fine-tune and adjust cytoskeletal arrays	323:467	Their organization and dynamics are co-ordinated by various microtubule-associated proteins (MAPs) that integrate environmental and developmental stimuli to fine-tune and adjust cytoskeletal arrays.
30407556	4	42	theme	biological	720:729	arg1	functions					731:739	biological functions	720:739	biological functions	720:739	Here, using a reverse genetics approach, we characterize Arabidopsis IQD5 in terms of its expression domains, subcellular localization, and biological functions.
30407556	4	43	theme	reverse	594:600	arg1	approach					611:618	a reverse genetics approach	592:618	a reverse genetics approach	592:618	Here, using a reverse genetics approach, we characterize Arabidopsis IQD5 in terms of its expression domains, subcellular localization, and biological functions.
30407556	4	44	theme	domains	681:687	arg1	terms					657:661	terms	657:661	terms of its expression domains, subcellular localization, and biological functions	657:739	Here, using a reverse genetics approach, we characterize Arabidopsis IQD5 in terms of its expression domains, subcellular localization, and biological functions.
30407556	4	45	theme	localization	702:713	arg1	terms					657:661	terms	657:661	terms of its expression domains, subcellular localization, and biological functions	657:739	Here, using a reverse genetics approach, we characterize Arabidopsis IQD5 in terms of its expression domains, subcellular localization, and biological functions.
30407556	1	46	theme	cell	230:233	arg1	proliferation					235:247	cell proliferation	230:247	cell proliferation	230:247	Plant microtubules form a highly dynamic intracellular network with important roles for regulating cell division, cell proliferation, and cell morphology.
30407556	3	47	theme	IQ67	470:473	arg1	IQD					483:485	IQD	483:485	IQD	483:485	IQ67 DOMAIN (IQD) proteins recently emerged as a class of plant-specific MAPs with largely unknown functions.
30407556	3	47	theme	IQ67	470:473	arg1	DOMAIN					475:480	IQ67 DOMAIN	470:480	IQ67 DOMAIN (IQD) proteins	470:495	IQ67 DOMAIN (IQD) proteins recently emerged as a class of plant-specific MAPs with largely unknown functions.
30407556	8	48	theme	first	1282:1286	arg1	evidence					1288:1295	first evidence	1282:1295	first evidence for important roles for calcium in regulation of PC morphogenesis	1282:1361	Lastly, we demonstrate IQD5-dependent recruitment of calmodulin calcium sensors to cortical microtubule arrays and provide first evidence for important roles for calcium in regulation of PC morphogenesis.
30407556	6	49	theme	lines	903:907	arg1	analysis					869:876	Our phenotypic analysis	854:876	Our phenotypic analysis of iqd5 loss-of-function lines	854:907	Our phenotypic analysis of iqd5 loss-of-function lines reveals functions of IQD5 in pavement cell (PC) shape morphogenesis.
30407556	7	50	theme	cellulose	1060:1068	arg1	deposition					1070:1079	cellulose deposition	1060:1079	cellulose deposition in anticlinal cell walls, which correlate with reduced anisotropic expansion	1060:1156	Histochemical analysis of cell wall composition further suggests reduced rates of cellulose deposition in anticlinal cell walls, which correlate with reduced anisotropic expansion.
30407556	2	51	theme	cytoskeletal	449:460	arg1	arrays					462:467	cytoskeletal arrays	449:467	cytoskeletal arrays	449:467	Their organization and dynamics are co-ordinated by various microtubule-associated proteins (MAPs) that integrate environmental and developmental stimuli to fine-tune and adjust cytoskeletal arrays.
30407556	0	52	theme	leaf	71:74	arg1	cells					85:89	leaf pavement cells	71:89	leaf pavement cells	71:89	Microtubule-associated protein IQ67 DOMAIN5 regulates morphogenesis of leaf pavement cells in Arabidopsis thaliana.
30407556	7	53	theme	cell	1095:1098	arg1	walls					1100:1104	anticlinal cell walls	1084:1104	anticlinal cell walls	1084:1104	Histochemical analysis of cell wall composition further suggests reduced rates of cellulose deposition in anticlinal cell walls, which correlate with reduced anisotropic expansion.
30407556	6	54	theme	loss-of-function	886:901	arg1	lines					903:907	iqd5 loss-of-function lines	881:907	iqd5 loss-of-function lines	881:907	Our phenotypic analysis of iqd5 loss-of-function lines reveals functions of IQD5 in pavement cell (PC) shape morphogenesis.
30407556	6	55	theme	IQD5	930:933	arg1	functions					917:925	functions	917:925	functions of IQD5 in pavement cell (PC) shape morphogenesis	917:975	Our phenotypic analysis of iqd5 loss-of-function lines reveals functions of IQD5 in pavement cell (PC) shape morphogenesis.
30407556	5	56	theme	cortical	825:832	arg1	arrays					846:851	cortical microtubule arrays	825:851	cortical microtubule arrays	825:851	We show that IQD5 is expressed mostly in vegetative tissues, where it localizes to cortical microtubule arrays.
30407556	7	57	theme	Histochemical	978:990	arg1	analysis					992:999	Histochemical analysis	978:999	Histochemical analysis of cell wall composition	978:1024	Histochemical analysis of cell wall composition further suggests reduced rates of cellulose deposition in anticlinal cell walls, which correlate with reduced anisotropic expansion.
30407556	9	58	theme	first	1443:1447	arg1	time					1449:1452	the first time	1439:1452	the first time	1439:1452	Our work identifies IQD5 as a novel player in PC shape regulation and, for the first time, links calcium signaling to developmental processes that regulate anisotropic growth in PCs.
30407556	6	59	theme	iqd5	881:884	arg1	lines					903:907	iqd5 loss-of-function lines	881:907	iqd5 loss-of-function lines	881:907	Our phenotypic analysis of iqd5 loss-of-function lines reveals functions of IQD5 in pavement cell (PC) shape morphogenesis.
30407556	3	60	theme	plant-specific	528:541	arg1	MAPs					543:546	plant-specific MAPs	528:546	plant-specific MAPs with largely unknown functions	528:577	IQ67 DOMAIN (IQD) proteins recently emerged as a class of plant-specific MAPs with largely unknown functions.
30407556	6	61	theme	shape	957:961	arg1	morphogenesis					963:975	pavement cell (PC) shape morphogenesis	938:975	pavement cell (PC) shape morphogenesis	938:975	Our phenotypic analysis of iqd5 loss-of-function lines reveals functions of IQD5 in pavement cell (PC) shape morphogenesis.
30407556	8	62	theme	morphogenesis	1349:1361	arg1	regulation					1332:1341	regulation	1332:1341	regulation of PC morphogenesis	1332:1361	Lastly, we demonstrate IQD5-dependent recruitment of calmodulin calcium sensors to cortical microtubule arrays and provide first evidence for important roles for calcium in regulation of PC morphogenesis.
30407556	9	63	theme	shape	1413:1417	arg1	regulation					1419:1428	PC shape regulation	1410:1428	PC shape regulation	1410:1428	Our work identifies IQD5 as a novel player in PC shape regulation and, for the first time, links calcium signaling to developmental processes that regulate anisotropic growth in PCs.
30407556	8	64	from	roles	1311:1315	arg1	regulation					1332:1341	regulation	1332:1341	regulation of PC morphogenesis	1332:1361	Lastly, we demonstrate IQD5-dependent recruitment of calmodulin calcium sensors to cortical microtubule arrays and provide first evidence for important roles for calcium in regulation of PC morphogenesis.
30407556	1	65	theme	Plant	116:120	arg1	microtubules					122:133	Plant microtubules	116:133	Plant microtubules	116:133	Plant microtubules form a highly dynamic intracellular network with important roles for regulating cell division, cell proliferation, and cell morphology.
30407556	7	66	from	rates	1051:1055	arg1	walls					1100:1104	anticlinal cell walls	1084:1104	anticlinal cell walls	1084:1104	Histochemical analysis of cell wall composition further suggests reduced rates of cellulose deposition in anticlinal cell walls, which correlate with reduced anisotropic expansion.
30407556	7	67	theme	anticlinal	1084:1093	arg1	walls					1100:1104	anticlinal cell walls	1084:1104	anticlinal cell walls	1084:1104	Histochemical analysis of cell wall composition further suggests reduced rates of cellulose deposition in anticlinal cell walls, which correlate with reduced anisotropic expansion.
30407556	1	68	theme	dynamic	149:155	arg1	network					171:177	a highly dynamic intracellular network	140:177	a highly dynamic intracellular network with important roles for regulating cell division, cell proliferation, and cell morphology	140:268	Plant microtubules form a highly dynamic intracellular network with important roles for regulating cell division, cell proliferation, and cell morphology.
30407556	8	69	theme	important	1301:1309	arg1	roles					1311:1315	important roles	1301:1315	important roles for calcium in regulation of PC morphogenesis	1301:1361	Lastly, we demonstrate IQD5-dependent recruitment of calmodulin calcium sensors to cortical microtubule arrays and provide first evidence for important roles for calcium in regulation of PC morphogenesis.
30407556	4	70	theme	Arabidopsis	637:647	arg1	IQD5					649:652	Arabidopsis IQD5	637:652	Arabidopsis IQD5	637:652	Here, using a reverse genetics approach, we characterize Arabidopsis IQD5 in terms of its expression domains, subcellular localization, and biological functions.
30407556	0	71	theme	cells	85:89	arg1	morphogenesis					54:66	morphogenesis	54:66	morphogenesis of leaf pavement cells in Arabidopsis thaliana	54:113	Microtubule-associated protein IQ67 DOMAIN5 regulates morphogenesis of leaf pavement cells in Arabidopsis thaliana.
30407556	4	72	theme	expression	670:679	arg1	domains					681:687	its expression domains	666:687	its expression domains	666:687	Here, using a reverse genetics approach, we characterize Arabidopsis IQD5 in terms of its expression domains, subcellular localization, and biological functions.
30407556	8	73	theme	PC	1346:1347	arg1	morphogenesis					1349:1361	PC morphogenesis	1346:1361	PC morphogenesis	1346:1361	Lastly, we demonstrate IQD5-dependent recruitment of calmodulin calcium sensors to cortical microtubule arrays and provide first evidence for important roles for calcium in regulation of PC morphogenesis.
30407556	7	74	theme	reduced	1043:1049	arg1	rates					1051:1055	reduced rates	1043:1055	reduced rates of cellulose deposition in anticlinal cell walls, which correlate with reduced anisotropic expansion	1043:1156	Histochemical analysis of cell wall composition further suggests reduced rates of cellulose deposition in anticlinal cell walls, which correlate with reduced anisotropic expansion.
30407556	1	75	theme	intracellular	157:169	arg1	network					171:177	a highly dynamic intracellular network	140:177	a highly dynamic intracellular network with important roles for regulating cell division, cell proliferation, and cell morphology	140:268	Plant microtubules form a highly dynamic intracellular network with important roles for regulating cell division, cell proliferation, and cell morphology.
30407556	9	76	from	growth	1532:1537	arg1	PCs					1542:1544	PCs	1542:1544	PCs	1542:1544	Our work identifies IQD5 as a novel player in PC shape regulation and, for the first time, links calcium signaling to developmental processes that regulate anisotropic growth in PCs.
30407556	1	77	theme	cell	254:257	arg1	morphology					259:268	cell morphology	254:268	cell morphology	254:268	Plant microtubules form a highly dynamic intracellular network with important roles for regulating cell division, cell proliferation, and cell morphology.
30407556	0	78	theme	pavement	76:83	arg1	cells					85:89	leaf pavement cells	71:89	leaf pavement cells	71:89	Microtubule-associated protein IQ67 DOMAIN5 regulates morphogenesis of leaf pavement cells in Arabidopsis thaliana.
30407556	7	79	from	deposition	1070:1079	arg1	walls					1100:1104	anticlinal cell walls	1084:1104	anticlinal cell walls	1084:1104	Histochemical analysis of cell wall composition further suggests reduced rates of cellulose deposition in anticlinal cell walls, which correlate with reduced anisotropic expansion.
30407556	7	80	theme	composition	1014:1024	arg1	analysis					992:999	Histochemical analysis	978:999	Histochemical analysis of cell wall composition	978:1024	Histochemical analysis of cell wall composition further suggests reduced rates of cellulose deposition in anticlinal cell walls, which correlate with reduced anisotropic expansion.
30407556	2	81	theme	developmental	403:415	arg1	stimuli					417:423	environmental and developmental stimuli	385:423	environmental and developmental stimuli to fine-tune	385:436	Their organization and dynamics are co-ordinated by various microtubule-associated proteins (MAPs) that integrate environmental and developmental stimuli to fine-tune and adjust cytoskeletal arrays.
30407556	7	82	from	walls	1100:1104	arg1	rates					1051:1055	reduced rates	1043:1055	reduced rates of cellulose deposition in anticlinal cell walls, which correlate with reduced anisotropic expansion	1043:1156	Histochemical analysis of cell wall composition further suggests reduced rates of cellulose deposition in anticlinal cell walls, which correlate with reduced anisotropic expansion.
31135330	9	0	theme	ll-2,6-diaminopimelic	1113:1133	arg1	acid					1135:1138	ll-2,6-diaminopimelic acid	1113:1138	ll-2,6-diaminopimelic acid	1113:1138	Strain HLT2-9T contained ll-2,6-diaminopimelic acid as the diamino acid in the cell-wall peptidoglycan.
31135330	9	0	theme	ll-2,6-diaminopimelic	1113:1133	arg1	acid					1155:1158	the diamino acid	1143:1158	the diamino acid in the cell-wall peptidoglycan	1143:1189	Strain HLT2-9T contained ll-2,6-diaminopimelic acid as the diamino acid in the cell-wall peptidoglycan.
31135330	7	1	theme	novel	965:969	arg1	species					971:977	a novel species	963:977	a novel species of the genus Nocardioides	963:1003	However, the low average nucleotide identity (85.6-87.9 %) and digital DNA-DNA hybridization values (26.4-30.2 %) of strain HLT2-9T to its three closest relatives demonstrated that it represents a novel species of the genus Nocardioides.
31135330	7	2	theme	digital	831:837	arg1	values					861:866	digital DNA-DNA hybridization values	831:866	digital DNA-DNA hybridization values	831:866	However, the low average nucleotide identity (85.6-87.9 %) and digital DNA-DNA hybridization values (26.4-30.2 %) of strain HLT2-9T to its three closest relatives demonstrated that it represents a novel species of the genus Nocardioides.
31135330	2	3	dep	China	264:268	arg1	PR					261:262	Sichuan Province, PR China	243:268	PR	261:262	A novel Gram-stain-positive, non-motile, rod-shaped, non-spore-forming bacterium, designated HLT2-9T, was isolated from the ice tongue surface of Hailuogou Glacier in Sichuan Province, PR China.
31135330	7	4	theme	strain	885:890	arg1	HLT2-9T					892:898	strain HLT2-9T	885:898	strain HLT2-9T	885:898	However, the low average nucleotide identity (85.6-87.9 %) and digital DNA-DNA hybridization values (26.4-30.2 %) of strain HLT2-9T to its three closest relatives demonstrated that it represents a novel species of the genus Nocardioides.
31135330	11	5	theme	DNA	1264:1266	arg1	%					1281:1281	70.65 mol%	1272:1281	70.65 mol%	1272:1281	The G+C content of the genomic DNA was 70.65 mol%.
31135330	11	5	theme	DNA	1264:1266	arg1	G+C content					1237:1247	The G+C content	1233:1247	The G+C content of the genomic DNA	1233:1266	The G+C content of the genomic DNA was 70.65 mol%.
31135330	7	6	theme	closest	913:919	arg1	relatives					921:929	three closest relatives	907:929	its three closest relatives	903:929	However, the low average nucleotide identity (85.6-87.9 %) and digital DNA-DNA hybridization values (26.4-30.2 %) of strain HLT2-9T to its three closest relatives demonstrated that it represents a novel species of the genus Nocardioides.
31135330	5	7	theme	16S	472:474	arg1	sequences					486:494	16S rRNA gene sequences	472:494	16S rRNA gene sequences	472:494	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain HLT2-9T belonged to the genus Nocardioides.
31135330	4	8	dep	optimum	354:360	arg1	20 °C					363:367	20 °C	363:367	20 °C	363:367	Growth occurred at 0-27 °C (optimum, 20 °C), pH 7.0-10.0 (pH 7.0) and in the presence of 0-2.0 % (w/v) NaCl (0 %).
31135330	7	9	theme	HLT2-9T	892:898	arg1	identity					804:811	the low average nucleotide identity	777:811	the low average nucleotide identity (85.6-87.9 %)	777:825	However, the low average nucleotide identity (85.6-87.9 %) and digital DNA-DNA hybridization values (26.4-30.2 %) of strain HLT2-9T to its three closest relatives demonstrated that it represents a novel species of the genus Nocardioides.
31135330	7	9	theme	HLT2-9T	892:898	arg1	%					824:824	85.6-87.9 %	814:824	85.6-87.9 %	814:824	However, the low average nucleotide identity (85.6-87.9 %) and digital DNA-DNA hybridization values (26.4-30.2 %) of strain HLT2-9T to its three closest relatives demonstrated that it represents a novel species of the genus Nocardioides.
31135330	7	9	theme	HLT2-9T	892:898	arg1	values					861:866	digital DNA-DNA hybridization values	831:866	digital DNA-DNA hybridization values	831:866	However, the low average nucleotide identity (85.6-87.9 %) and digital DNA-DNA hybridization values (26.4-30.2 %) of strain HLT2-9T to its three closest relatives demonstrated that it represents a novel species of the genus Nocardioides.
31135330	7	9	theme	HLT2-9T	892:898	arg1	%					879:879	26.4-30.2 %	869:879	26.4-30.2 %	869:879	However, the low average nucleotide identity (85.6-87.9 %) and digital DNA-DNA hybridization values (26.4-30.2 %) of strain HLT2-9T to its three closest relatives demonstrated that it represents a novel species of the genus Nocardioides.
31135330	4	10	theme	%	421:421	arg1	NaCl					429:432	0-2.0 % (w/v) NaCl	415:432	0-2.0 % (w/v) NaCl (0 %)	415:438	Growth occurred at 0-27 °C (optimum, 20 °C), pH 7.0-10.0 (pH 7.0) and in the presence of 0-2.0 % (w/v) NaCl (0 %).
31135330	4	10	theme	%	421:421	arg1	%					437:437	0 %	435:437	0 %	435:437	Growth occurred at 0-27 °C (optimum, 20 °C), pH 7.0-10.0 (pH 7.0) and in the presence of 0-2.0 % (w/v) NaCl (0 %).
31135330	5	11	theme	rRNA	476:479	arg1	sequences					486:494	16S rRNA gene sequences	472:494	16S rRNA gene sequences	472:494	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain HLT2-9T belonged to the genus Nocardioides.
31135330	8	12	dep	C17 	1060:1063	arg1	iso-C16 					1075:1082	iso-C16 	1075:1082	iso-C16 	1075:1082	The major cellular fatty acids of strain HLT2-9T were C17 : 1ω8c and iso-C16 : 0.
31135330	8	12	dep	C17 	1060:1063	arg1	 1ω8c					1065:1069	 1ω8c	1065:1069	 1ω8c	1065:1069	The major cellular fatty acids of strain HLT2-9T were C17 : 1ω8c and iso-C16 : 0.
31135330	8	12	dep	C17 	1060:1063	arg1	 0					1084:1085	 0	1084:1085	C17 : 1ω8c and iso-C16 : 0	1060:1085	The major cellular fatty acids of strain HLT2-9T were C17 : 1ω8c and iso-C16 : 0.
31135330	7	13	theme	DNA-DNA	839:845	arg1	values					861:866	digital DNA-DNA hybridization values	831:866	digital DNA-DNA hybridization values	831:866	However, the low average nucleotide identity (85.6-87.9 %) and digital DNA-DNA hybridization values (26.4-30.2 %) of strain HLT2-9T to its three closest relatives demonstrated that it represents a novel species of the genus Nocardioides.
31135330	5	14	theme	genus	539:543	arg1	Nocardioides					545:556	the genus Nocardioides	535:556	the genus Nocardioides	535:556	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain HLT2-9T belonged to the genus Nocardioides.
31135330	5	15	theme	gene	481:484	arg1	sequences					486:494	16S rRNA gene sequences	472:494	16S rRNA gene sequences	472:494	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain HLT2-9T belonged to the genus Nocardioides.
31135330	6	16	theme	similarities	589:600	arg1	level					571:575	The highest level	559:575	The highest level of sequence similarities	559:600	The highest level of sequence similarities were found to Nocardioides glacieisoli CGMCC1.11097T (99.24 %), Nocardioides oleivorans CGMCC 4.6882T (98.54 %) and Nocardioides ganghwensis CGMCC 4.6875T (98.54 %).
31135330	9	17	contain	contained	1103:1111	arg2	acid					1155:1158	the diamino acid	1143:1158	the diamino acid in the cell-wall peptidoglycan	1143:1189	Strain HLT2-9T contained ll-2,6-diaminopimelic acid as the diamino acid in the cell-wall peptidoglycan.
31135330	9	17	contain	contained	1103:1111	arg2	acid					1135:1138	ll-2,6-diaminopimelic acid	1113:1138	ll-2,6-diaminopimelic acid	1113:1138	Strain HLT2-9T contained ll-2,6-diaminopimelic acid as the diamino acid in the cell-wall peptidoglycan.
31135330	9	17	contain	contained	1103:1111	arg1	HLT2-9T					1095:1101	Strain HLT2-9T	1088:1101	Strain HLT2-9T	1088:1101	Strain HLT2-9T contained ll-2,6-diaminopimelic acid as the diamino acid in the cell-wall peptidoglycan.
31135330	8	18	theme	cellular	1016:1023	arg1	C17 					1060:1063	C17 	1060:1063	C17 : 1ω8c and iso-C16 : 0	1060:1085	The major cellular fatty acids of strain HLT2-9T were C17 : 1ω8c and iso-C16 : 0.
31135330	8	18	theme	cellular	1016:1023	arg1	acids					1031:1035	The major cellular fatty acids	1006:1035	The major cellular fatty acids of strain HLT2-9T	1006:1053	The major cellular fatty acids of strain HLT2-9T were C17 : 1ω8c and iso-C16 : 0.
31135330	9	19	theme	diamino	1147:1153	arg1	acid					1135:1138	ll-2,6-diaminopimelic acid	1113:1138	ll-2,6-diaminopimelic acid	1113:1138	Strain HLT2-9T contained ll-2,6-diaminopimelic acid as the diamino acid in the cell-wall peptidoglycan.
31135330	9	19	theme	diamino	1147:1153	arg1	acid					1155:1158	the diamino acid	1143:1158	the diamino acid in the cell-wall peptidoglycan	1143:1189	Strain HLT2-9T contained ll-2,6-diaminopimelic acid as the diamino acid in the cell-wall peptidoglycan.
31135330	6	20	theme	sequence	580:587	arg1	similarities					589:600	sequence similarities	580:600	sequence similarities	580:600	The highest level of sequence similarities were found to Nocardioides glacieisoli CGMCC1.11097T (99.24 %), Nocardioides oleivorans CGMCC 4.6882T (98.54 %) and Nocardioides ganghwensis CGMCC 4.6875T (98.54 %).
31135330	8	21	theme	major	1010:1014	arg1	C17 					1060:1063	C17 	1060:1063	C17 : 1ω8c and iso-C16 : 0	1060:1085	The major cellular fatty acids of strain HLT2-9T were C17 : 1ω8c and iso-C16 : 0.
31135330	8	21	theme	major	1010:1014	arg1	acids					1031:1035	The major cellular fatty acids	1006:1035	The major cellular fatty acids of strain HLT2-9T	1006:1053	The major cellular fatty acids of strain HLT2-9T were C17 : 1ω8c and iso-C16 : 0.
31135330	12	22	theme	phylogenetic	1347:1358	arg1	analyses					1360:1367	the phenotypic, genotypic and phylogenetic analyses	1317:1367	the phenotypic, genotypic and phylogenetic analyses	1317:1367	Based on evidence collected from the phenotypic, genotypic and phylogenetic analyses, a novel species Nocardioideszhouii sp.
31135330	12	22	theme	phylogenetic	1347:1358	arg1	species					1378:1384	a novel species Nocardioideszhouii sp	1370:1406	a novel species Nocardioideszhouii sp	1370:1406	Based on evidence collected from the phenotypic, genotypic and phylogenetic analyses, a novel species Nocardioideszhouii sp.
31135330	5	23	theme	Phylogenetic	441:452	arg1	analysis					454:461	Phylogenetic analysis	441:461	Phylogenetic analysis based on 16S rRNA gene sequences	441:494	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain HLT2-9T belonged to the genus Nocardioides.
31135330	2	24	theme	Province	251:258	arg1	China					264:268	Sichuan Province, PR China	243:268	China	264:268	A novel Gram-stain-positive, non-motile, rod-shaped, non-spore-forming bacterium, designated HLT2-9T, was isolated from the ice tongue surface of Hailuogou Glacier in Sichuan Province, PR China.
31135330	6	25	dep	Nocardioides	666:677	arg1	oleivorans					679:688	oleivorans	679:688	oleivorans	679:688	The highest level of sequence similarities were found to Nocardioides glacieisoli CGMCC1.11097T (99.24 %), Nocardioides oleivorans CGMCC 4.6882T (98.54 %) and Nocardioides ganghwensis CGMCC 4.6875T (98.54 %).
31135330	11	26	theme	genomic	1256:1262	arg1	DNA					1264:1266	the genomic DNA	1252:1266	the genomic DNA	1252:1266	The G+C content of the genomic DNA was 70.65 mol%.
31135330	0	27	theme	zhouii	13:18	arg1	sp					20:21	zhouii sp	13:21	zhouii sp	13:21	Nocardioides zhouii sp.
31135330	5	28	theme	strain	508:513	arg1	HLT2-9T					515:521	strain HLT2-9T	508:521	strain HLT2-9T	508:521	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain HLT2-9T belonged to the genus Nocardioides.
31135330	2	29	from	surface	211:217	arg1	China					264:268	Sichuan Province, PR China	243:268	China	264:268	A novel Gram-stain-positive, non-motile, rod-shaped, non-spore-forming bacterium, designated HLT2-9T, was isolated from the ice tongue surface of Hailuogou Glacier in Sichuan Province, PR China.
31135330	7	30	theme	nucleotide	793:802	arg1	identity					804:811	the low average nucleotide identity	777:811	the low average nucleotide identity (85.6-87.9 %)	777:825	However, the low average nucleotide identity (85.6-87.9 %) and digital DNA-DNA hybridization values (26.4-30.2 %) of strain HLT2-9T to its three closest relatives demonstrated that it represents a novel species of the genus Nocardioides.
31135330	7	30	theme	nucleotide	793:802	arg1	%					824:824	85.6-87.9 %	814:824	85.6-87.9 %	814:824	However, the low average nucleotide identity (85.6-87.9 %) and digital DNA-DNA hybridization values (26.4-30.2 %) of strain HLT2-9T to its three closest relatives demonstrated that it represents a novel species of the genus Nocardioides.
31135330	7	30	theme	nucleotide	793:802	arg1	%					879:879	26.4-30.2 %	869:879	26.4-30.2 %	869:879	However, the low average nucleotide identity (85.6-87.9 %) and digital DNA-DNA hybridization values (26.4-30.2 %) of strain HLT2-9T to its three closest relatives demonstrated that it represents a novel species of the genus Nocardioides.
31135330	8	31	theme	fatty	1025:1029	arg1	C17 					1060:1063	C17 	1060:1063	C17 : 1ω8c and iso-C16 : 0	1060:1085	The major cellular fatty acids of strain HLT2-9T were C17 : 1ω8c and iso-C16 : 0.
31135330	8	31	theme	fatty	1025:1029	arg1	acids					1031:1035	The major cellular fatty acids	1006:1035	The major cellular fatty acids of strain HLT2-9T	1006:1053	The major cellular fatty acids of strain HLT2-9T were C17 : 1ω8c and iso-C16 : 0.
31135330	7	32	theme	low	781:783	arg1	identity					804:811	the low average nucleotide identity	777:811	the low average nucleotide identity (85.6-87.9 %)	777:825	However, the low average nucleotide identity (85.6-87.9 %) and digital DNA-DNA hybridization values (26.4-30.2 %) of strain HLT2-9T to its three closest relatives demonstrated that it represents a novel species of the genus Nocardioides.
31135330	7	32	theme	low	781:783	arg1	%					824:824	85.6-87.9 %	814:824	85.6-87.9 %	814:824	However, the low average nucleotide identity (85.6-87.9 %) and digital DNA-DNA hybridization values (26.4-30.2 %) of strain HLT2-9T to its three closest relatives demonstrated that it represents a novel species of the genus Nocardioides.
31135330	7	32	theme	low	781:783	arg1	%					879:879	26.4-30.2 %	869:879	26.4-30.2 %	869:879	However, the low average nucleotide identity (85.6-87.9 %) and digital DNA-DNA hybridization values (26.4-30.2 %) of strain HLT2-9T to its three closest relatives demonstrated that it represents a novel species of the genus Nocardioides.
31135330	6	33	theme	highest	563:569	arg1	level					571:575	The highest level	559:575	The highest level of sequence similarities	559:600	The highest level of sequence similarities were found to Nocardioides glacieisoli CGMCC1.11097T (99.24 %), Nocardioides oleivorans CGMCC 4.6882T (98.54 %) and Nocardioides ganghwensis CGMCC 4.6875T (98.54 %).
31135330	2	34	attach	isolated	182:189	arg2	bacterium					147:155	A novel Gram-stain-positive, non-motile, rod-shaped, non-spore-forming bacterium	76:155	A novel Gram-stain-positive, non-motile, rod-shaped, non-spore-forming bacterium	76:155	A novel Gram-stain-positive, non-motile, rod-shaped, non-spore-forming bacterium, designated HLT2-9T, was isolated from the ice tongue surface of Hailuogou Glacier in Sichuan Province, PR China.
31135330	2	34	attach	isolated	182:189	arg1	surface					211:217	the ice tongue surface	196:217	the ice tongue surface of Hailuogou Glacier in Sichuan Province, PR China	196:268	A novel Gram-stain-positive, non-motile, rod-shaped, non-spore-forming bacterium, designated HLT2-9T, was isolated from the ice tongue surface of Hailuogou Glacier in Sichuan Province, PR China.
31135330	8	35	theme	strain	1040:1045	arg1	HLT2-9T					1047:1053	strain HLT2-9T	1040:1053	strain HLT2-9T	1040:1053	The major cellular fatty acids of strain HLT2-9T were C17 : 1ω8c and iso-C16 : 0.
31135330	9	36	theme	cell-wall	1167:1175	arg1	peptidoglycan					1177:1189	the cell-wall peptidoglycan	1163:1189	the cell-wall peptidoglycan	1163:1189	Strain HLT2-9T contained ll-2,6-diaminopimelic acid as the diamino acid in the cell-wall peptidoglycan.
31135330	7	37	theme	average	785:791	arg1	identity					804:811	the low average nucleotide identity	777:811	the low average nucleotide identity (85.6-87.9 %)	777:825	However, the low average nucleotide identity (85.6-87.9 %) and digital DNA-DNA hybridization values (26.4-30.2 %) of strain HLT2-9T to its three closest relatives demonstrated that it represents a novel species of the genus Nocardioides.
31135330	7	37	theme	average	785:791	arg1	%					824:824	85.6-87.9 %	814:824	85.6-87.9 %	814:824	However, the low average nucleotide identity (85.6-87.9 %) and digital DNA-DNA hybridization values (26.4-30.2 %) of strain HLT2-9T to its three closest relatives demonstrated that it represents a novel species of the genus Nocardioides.
31135330	7	37	theme	average	785:791	arg1	%					879:879	26.4-30.2 %	869:879	26.4-30.2 %	869:879	However, the low average nucleotide identity (85.6-87.9 %) and digital DNA-DNA hybridization values (26.4-30.2 %) of strain HLT2-9T to its three closest relatives demonstrated that it represents a novel species of the genus Nocardioides.
31135330	3	38	dep	cream	294:298	arg1	yellow					300:305	yellow	300:305	yellow	300:305	Colonies of cells were cream yellow, convex and round.
31135330	3	38	dep	cream	294:298	arg1	round					319:323	round	319:323	round	319:323	Colonies of cells were cream yellow, convex and round.
31135330	3	38	dep	cream	294:298	arg1	cream					294:298	cream	294:298	cream yellow, convex and round	294:323	Colonies of cells were cream yellow, convex and round.
31135330	3	38	dep	cream	294:298	arg1	convex					308:313	convex	308:313	convex	308:313	Colonies of cells were cream yellow, convex and round.
31135330	12	39	theme	phenotypic	1321:1330	arg1	analyses					1360:1367	the phenotypic, genotypic and phylogenetic analyses	1317:1367	the phenotypic, genotypic and phylogenetic analyses	1317:1367	Based on evidence collected from the phenotypic, genotypic and phylogenetic analyses, a novel species Nocardioideszhouii sp.
31135330	12	39	theme	phenotypic	1321:1330	arg1	species					1378:1384	a novel species Nocardioideszhouii sp	1370:1406	a novel species Nocardioideszhouii sp	1370:1406	Based on evidence collected from the phenotypic, genotypic and phylogenetic analyses, a novel species Nocardioideszhouii sp.
31135330	12	40	dep	species	1378:1384	arg1	sp					1405:1406	Nocardioideszhouii sp	1386:1406	a novel species Nocardioideszhouii sp	1370:1406	Based on evidence collected from the phenotypic, genotypic and phylogenetic analyses, a novel species Nocardioideszhouii sp.
31135330	11	41	theme	70.65 mol	1272:1280	arg1	%					1281:1281	70.65 mol%	1272:1281	70.65 mol%	1272:1281	The G+C content of the genomic DNA was 70.65 mol%.
31135330	11	41	theme	70.65 mol	1272:1280	arg1	G+C content					1237:1247	The G+C content	1233:1247	The G+C content of the genomic DNA	1233:1266	The G+C content of the genomic DNA was 70.65 mol%.
31135330	7	42	theme	genus	986:990	arg1	Nocardioides					992:1003	the genus Nocardioides	982:1003	the genus Nocardioides	982:1003	However, the low average nucleotide identity (85.6-87.9 %) and digital DNA-DNA hybridization values (26.4-30.2 %) of strain HLT2-9T to its three closest relatives demonstrated that it represents a novel species of the genus Nocardioides.
31135330	6	43	theme	CGMCC	743:747	arg1	%					764:764	98.54 %	758:764	98.54 %	758:764	The highest level of sequence similarities were found to Nocardioides glacieisoli CGMCC1.11097T (99.24 %), Nocardioides oleivorans CGMCC 4.6882T (98.54 %) and Nocardioides ganghwensis CGMCC 4.6875T (98.54 %).
31135330	6	43	theme	CGMCC	743:747	arg1	4.6875T					749:755	Nocardioides ganghwensis CGMCC 4.6875T	718:755	Nocardioides ganghwensis CGMCC 4.6875T (98.54 %)	718:765	The highest level of sequence similarities were found to Nocardioides glacieisoli CGMCC1.11097T (99.24 %), Nocardioides oleivorans CGMCC 4.6882T (98.54 %) and Nocardioides ganghwensis CGMCC 4.6875T (98.54 %).
31135330	7	44	theme	Nocardioides	992:1003	arg1	species					971:977	a novel species	963:977	a novel species of the genus Nocardioides	963:1003	However, the low average nucleotide identity (85.6-87.9 %) and digital DNA-DNA hybridization values (26.4-30.2 %) of strain HLT2-9T to its three closest relatives demonstrated that it represents a novel species of the genus Nocardioides.
31135330	2	45	theme	novel	78:82	arg1	bacterium					147:155	A novel Gram-stain-positive, non-motile, rod-shaped, non-spore-forming bacterium	76:155	A novel Gram-stain-positive, non-motile, rod-shaped, non-spore-forming bacterium	76:155	A novel Gram-stain-positive, non-motile, rod-shaped, non-spore-forming bacterium, designated HLT2-9T, was isolated from the ice tongue surface of Hailuogou Glacier in Sichuan Province, PR China.
31135330	8	46	theme	HLT2-9T	1047:1053	arg1	C17 					1060:1063	C17 	1060:1063	C17 : 1ω8c and iso-C16 : 0	1060:1085	The major cellular fatty acids of strain HLT2-9T were C17 : 1ω8c and iso-C16 : 0.
31135330	8	46	theme	HLT2-9T	1047:1053	arg1	acids					1031:1035	The major cellular fatty acids	1006:1035	The major cellular fatty acids of strain HLT2-9T	1006:1053	The major cellular fatty acids of strain HLT2-9T were C17 : 1ω8c and iso-C16 : 0.
31135330	12	47	theme	genotypic	1333:1341	arg1	analyses					1360:1367	the phenotypic, genotypic and phylogenetic analyses	1317:1367	the phenotypic, genotypic and phylogenetic analyses	1317:1367	Based on evidence collected from the phenotypic, genotypic and phylogenetic analyses, a novel species Nocardioideszhouii sp.
31135330	12	47	theme	genotypic	1333:1341	arg1	species					1378:1384	a novel species Nocardioideszhouii sp	1370:1406	a novel species Nocardioideszhouii sp	1370:1406	Based on evidence collected from the phenotypic, genotypic and phylogenetic analyses, a novel species Nocardioideszhouii sp.
31135330	4	48	theme	w/v	424:426	arg1	NaCl					429:432	0-2.0 % (w/v) NaCl	415:432	0-2.0 % (w/v) NaCl (0 %)	415:438	Growth occurred at 0-27 °C (optimum, 20 °C), pH 7.0-10.0 (pH 7.0) and in the presence of 0-2.0 % (w/v) NaCl (0 %).
31135330	4	48	theme	w/v	424:426	arg1	%					437:437	0 %	435:437	0 %	435:437	Growth occurred at 0-27 °C (optimum, 20 °C), pH 7.0-10.0 (pH 7.0) and in the presence of 0-2.0 % (w/v) NaCl (0 %).
31135330	6	49	theme	CGMCC	690:694	arg1	4.6882T					696:702	Nocardioides oleivorans CGMCC 4.6882T	666:702	Nocardioides oleivorans CGMCC 4.6882T (98.54 %)	666:712	The highest level of sequence similarities were found to Nocardioides glacieisoli CGMCC1.11097T (99.24 %), Nocardioides oleivorans CGMCC 4.6882T (98.54 %) and Nocardioides ganghwensis CGMCC 4.6875T (98.54 %).
31135330	6	49	theme	CGMCC	690:694	arg1	%					711:711	98.54 %	705:711	98.54 %	705:711	The highest level of sequence similarities were found to Nocardioides glacieisoli CGMCC1.11097T (99.24 %), Nocardioides oleivorans CGMCC 4.6882T (98.54 %) and Nocardioides ganghwensis CGMCC 4.6875T (98.54 %).
31135330	6	50	theme	Nocardioides	718:729	arg1	%					764:764	98.54 %	758:764	98.54 %	758:764	The highest level of sequence similarities were found to Nocardioides glacieisoli CGMCC1.11097T (99.24 %), Nocardioides oleivorans CGMCC 4.6882T (98.54 %) and Nocardioides ganghwensis CGMCC 4.6875T (98.54 %).
31135330	6	50	theme	Nocardioides	718:729	arg1	4.6875T					749:755	Nocardioides ganghwensis CGMCC 4.6875T	718:755	Nocardioides ganghwensis CGMCC 4.6875T (98.54 %)	718:765	The highest level of sequence similarities were found to Nocardioides glacieisoli CGMCC1.11097T (99.24 %), Nocardioides oleivorans CGMCC 4.6882T (98.54 %) and Nocardioides ganghwensis CGMCC 4.6875T (98.54 %).
31135330	2	51	theme	tongue	204:209	arg1	surface					211:217	the ice tongue surface	196:217	the ice tongue surface of Hailuogou Glacier in Sichuan Province, PR China	196:268	A novel Gram-stain-positive, non-motile, rod-shaped, non-spore-forming bacterium, designated HLT2-9T, was isolated from the ice tongue surface of Hailuogou Glacier in Sichuan Province, PR China.
31135330	2	52	theme	Gram-stain-positive	84:102	arg1	bacterium					147:155	A novel Gram-stain-positive, non-motile, rod-shaped, non-spore-forming bacterium	76:155	A novel Gram-stain-positive, non-motile, rod-shaped, non-spore-forming bacterium	76:155	A novel Gram-stain-positive, non-motile, rod-shaped, non-spore-forming bacterium, designated HLT2-9T, was isolated from the ice tongue surface of Hailuogou Glacier in Sichuan Province, PR China.
31135330	3	53	theme	cells	283:287	arg1	Colonies					271:278	Colonies	271:278	Colonies of cells	271:287	Colonies of cells were cream yellow, convex and round.
31135330	2	54	theme	ice	200:202	arg1	surface					211:217	the ice tongue surface	196:217	the ice tongue surface of Hailuogou Glacier in Sichuan Province, PR China	196:268	A novel Gram-stain-positive, non-motile, rod-shaped, non-spore-forming bacterium, designated HLT2-9T, was isolated from the ice tongue surface of Hailuogou Glacier in Sichuan Province, PR China.
31135330	6	55	dep	Nocardioides	718:729	arg1	ganghwensis					731:741	ganghwensis	731:741	ganghwensis	731:741	The highest level of sequence similarities were found to Nocardioides glacieisoli CGMCC1.11097T (99.24 %), Nocardioides oleivorans CGMCC 4.6882T (98.54 %) and Nocardioides ganghwensis CGMCC 4.6875T (98.54 %).
31135330	4	56	theme	NaCl	429:432	arg1	presence					403:410	the presence	399:410	the presence of 0-2.0 % (w/v) NaCl (0 %)	399:438	Growth occurred at 0-27 °C (optimum, 20 °C), pH 7.0-10.0 (pH 7.0) and in the presence of 0-2.0 % (w/v) NaCl (0 %).
31135330	13	57	theme	1.11084T=NBRC	1448:1460	arg1	109783T					1462:1468	=CGMCC 1.11084T=NBRC 109783T	1441:1468	=CGMCC 1.11084T=NBRC 109783T	1441:1468	nov. is proposed, with HLT2-9T (=CGMCC 1.11084T=NBRC 109783T) as the type strain.
31135330	13	57	theme	1.11084T=NBRC	1448:1460	arg1	HLT2-9T					1432:1438	HLT2-9T	1432:1438	HLT2-9T (=CGMCC 1.11084T=NBRC 109783T) as the type strain	1432:1488	nov. is proposed, with HLT2-9T (=CGMCC 1.11084T=NBRC 109783T) as the type strain.
31135330	6	58	theme	Nocardioides	666:677	arg1	4.6882T					696:702	Nocardioides oleivorans CGMCC 4.6882T	666:702	Nocardioides oleivorans CGMCC 4.6882T (98.54 %)	666:712	The highest level of sequence similarities were found to Nocardioides glacieisoli CGMCC1.11097T (99.24 %), Nocardioides oleivorans CGMCC 4.6882T (98.54 %) and Nocardioides ganghwensis CGMCC 4.6875T (98.54 %).
31135330	6	58	theme	Nocardioides	666:677	arg1	%					711:711	98.54 %	705:711	98.54 %	705:711	The highest level of sequence similarities were found to Nocardioides glacieisoli CGMCC1.11097T (99.24 %), Nocardioides oleivorans CGMCC 4.6882T (98.54 %) and Nocardioides ganghwensis CGMCC 4.6875T (98.54 %).
31135330	2	59	theme	Sichuan	243:249	arg1	China					264:268	Sichuan Province, PR China	243:268	China	264:268	A novel Gram-stain-positive, non-motile, rod-shaped, non-spore-forming bacterium, designated HLT2-9T, was isolated from the ice tongue surface of Hailuogou Glacier in Sichuan Province, PR China.
31135330	6	60	theme	glacieisoli	629:639	arg1	CGMCC1.11097T					641:653	Nocardioides glacieisoli CGMCC1.11097T	616:653	Nocardioides glacieisoli CGMCC1.11097T (99.24 %)	616:663	The highest level of sequence similarities were found to Nocardioides glacieisoli CGMCC1.11097T (99.24 %), Nocardioides oleivorans CGMCC 4.6882T (98.54 %) and Nocardioides ganghwensis CGMCC 4.6875T (98.54 %).
31135330	6	60	theme	glacieisoli	629:639	arg1	%					662:662	99.24 %	656:662	99.24 %	656:662	The highest level of sequence similarities were found to Nocardioides glacieisoli CGMCC1.11097T (99.24 %), Nocardioides oleivorans CGMCC 4.6882T (98.54 %) and Nocardioides ganghwensis CGMCC 4.6875T (98.54 %).
31135330	12	61	theme	novel	1372:1376	arg1	analyses					1360:1367	the phenotypic, genotypic and phylogenetic analyses	1317:1367	the phenotypic, genotypic and phylogenetic analyses	1317:1367	Based on evidence collected from the phenotypic, genotypic and phylogenetic analyses, a novel species Nocardioideszhouii sp.
31135330	12	61	theme	novel	1372:1376	arg1	species					1378:1384	a novel species Nocardioideszhouii sp	1370:1406	a novel species Nocardioideszhouii sp	1370:1406	Based on evidence collected from the phenotypic, genotypic and phylogenetic analyses, a novel species Nocardioideszhouii sp.
31135330	13	62	theme	=CGMCC	1441:1446	arg1	109783T					1462:1468	=CGMCC 1.11084T=NBRC 109783T	1441:1468	=CGMCC 1.11084T=NBRC 109783T	1441:1468	nov. is proposed, with HLT2-9T (=CGMCC 1.11084T=NBRC 109783T) as the type strain.
31135330	13	62	theme	=CGMCC	1441:1446	arg1	HLT2-9T					1432:1438	HLT2-9T	1432:1438	HLT2-9T (=CGMCC 1.11084T=NBRC 109783T) as the type strain	1432:1488	nov. is proposed, with HLT2-9T (=CGMCC 1.11084T=NBRC 109783T) as the type strain.
31135330	9	63	theme	Strain	1088:1093	arg1	HLT2-9T					1095:1101	Strain HLT2-9T	1088:1101	Strain HLT2-9T	1088:1101	Strain HLT2-9T contained ll-2,6-diaminopimelic acid as the diamino acid in the cell-wall peptidoglycan.
31135330	0	64	dep	sp	20:21	arg1	Nocardioides					0:11	Nocardioides	0:11	Nocardioides	0:11	Nocardioides zhouii sp.
31135330	6	65	theme	Nocardioides	616:627	arg1	CGMCC1.11097T					641:653	Nocardioides glacieisoli CGMCC1.11097T	616:653	Nocardioides glacieisoli CGMCC1.11097T (99.24 %)	616:663	The highest level of sequence similarities were found to Nocardioides glacieisoli CGMCC1.11097T (99.24 %), Nocardioides oleivorans CGMCC 4.6882T (98.54 %) and Nocardioides ganghwensis CGMCC 4.6875T (98.54 %).
31135330	6	65	theme	Nocardioides	616:627	arg1	%					662:662	99.24 %	656:662	99.24 %	656:662	The highest level of sequence similarities were found to Nocardioides glacieisoli CGMCC1.11097T (99.24 %), Nocardioides oleivorans CGMCC 4.6882T (98.54 %) and Nocardioides ganghwensis CGMCC 4.6875T (98.54 %).
31135330	2	66	theme	Glacier	232:238	arg1	surface					211:217	the ice tongue surface	196:217	the ice tongue surface of Hailuogou Glacier in Sichuan Province, PR China	196:268	A novel Gram-stain-positive, non-motile, rod-shaped, non-spore-forming bacterium, designated HLT2-9T, was isolated from the ice tongue surface of Hailuogou Glacier in Sichuan Province, PR China.
31135330	7	67	theme	hybridization	847:859	arg1	values					861:866	digital DNA-DNA hybridization values	831:866	digital DNA-DNA hybridization values	831:866	However, the low average nucleotide identity (85.6-87.9 %) and digital DNA-DNA hybridization values (26.4-30.2 %) of strain HLT2-9T to its three closest relatives demonstrated that it represents a novel species of the genus Nocardioides.
31135330	13	68	theme	type	1478:1481	arg1	strain					1483:1488	the type strain	1474:1488	the type strain	1474:1488	nov. is proposed, with HLT2-9T (=CGMCC 1.11084T=NBRC 109783T) as the type strain.
31135330	12	69	theme	Nocardioideszhouii	1386:1403	arg1	sp					1405:1406	Nocardioideszhouii sp	1386:1406	a novel species Nocardioideszhouii sp	1370:1406	Based on evidence collected from the phenotypic, genotypic and phylogenetic analyses, a novel species Nocardioideszhouii sp.
31135330	4	70	theme	0-2.0 	415:420	arg1	NaCl					429:432	0-2.0 % (w/v) NaCl	415:432	0-2.0 % (w/v) NaCl (0 %)	415:438	Growth occurred at 0-27 °C (optimum, 20 °C), pH 7.0-10.0 (pH 7.0) and in the presence of 0-2.0 % (w/v) NaCl (0 %).
31135330	4	70	theme	0-2.0 	415:420	arg1	%					437:437	0 %	435:437	0 %	435:437	Growth occurred at 0-27 °C (optimum, 20 °C), pH 7.0-10.0 (pH 7.0) and in the presence of 0-2.0 % (w/v) NaCl (0 %).
31135330	10	71	theme	predominant	1196:1206	arg1	MK-8					1223:1226	MK-8	1223:1226	MK-8(H4)	1223:1230	The predominant menaquinone is MK-8(H4).
31135330	10	71	theme	predominant	1196:1206	arg1	menaquinone					1208:1218	The predominant menaquinone	1192:1218	The predominant menaquinone	1192:1218	The predominant menaquinone is MK-8(H4).
31135330	9	72	from	acid	1155:1158	arg1	peptidoglycan					1177:1189	the cell-wall peptidoglycan	1163:1189	the cell-wall peptidoglycan	1163:1189	Strain HLT2-9T contained ll-2,6-diaminopimelic acid as the diamino acid in the cell-wall peptidoglycan.
31135330	2	73	dep	Gram-stain-positive	84:102	arg1	non-motile					105:114	non-motile	105:114	non-motile	105:114	A novel Gram-stain-positive, non-motile, rod-shaped, non-spore-forming bacterium, designated HLT2-9T, was isolated from the ice tongue surface of Hailuogou Glacier in Sichuan Province, PR China.
31135330	2	73	dep	Gram-stain-positive	84:102	arg1	non-spore-forming					129:145	non-spore-forming	129:145	non-spore-forming	129:145	A novel Gram-stain-positive, non-motile, rod-shaped, non-spore-forming bacterium, designated HLT2-9T, was isolated from the ice tongue surface of Hailuogou Glacier in Sichuan Province, PR China.
31135330	2	73	dep	Gram-stain-positive	84:102	arg1	rod-shaped					117:126	rod-shaped	117:126	rod-shaped	117:126	A novel Gram-stain-positive, non-motile, rod-shaped, non-spore-forming bacterium, designated HLT2-9T, was isolated from the ice tongue surface of Hailuogou Glacier in Sichuan Province, PR China.
30226463	10	0	theme	relatedness	1523:1533	arg1	values					1535:1540	a relatedness values	1521:1540	a relatedness values of only 39.8, 26.3 and 42.8 %, respectively	1521:1584	DNA-DNA hybridization of strain SMB4T with S. halochares DSM 21373T, S. halotolerans S7T and S. quigdaonense DSM 21621T showed a relatedness values of only 39.8, 26.3 and 42.8 %, respectively.
30226463	8	1	theme	similarity	980:989	arg1	search					991:996	A blast sequence similarity search	963:996	A blast sequence similarity search based on 16S rRNA gene sequence	963:1028	A blast sequence similarity search based on 16S rRNA gene sequence indicated that Salibacterium halochares, Salibacterium halotolerans and Salibacterium qingdaonense were the nearest phylogenetic neighbours, with a pair-wise sequence similarities of 98.4, 98.2 and 97.0 % respectively.
30226463	7	2	theme	SMB4T	942:946	arg1	G+C content					920:930	The DNA G+C content	912:930	The DNA G+C content of strain SMB4T	912:946	The DNA G+C content of strain SMB4T was 49.1 mol%.
30226463	7	2	theme	SMB4T	942:946	arg1	%					960:960	49.1 mol%	952:960	49.1 mol%	952:960	The DNA G+C content of strain SMB4T was 49.1 mol%.
30226463	5	3	with	acids	589:593	arg1	abundance					608:616	a high abundance	601:616	a high abundance of anteiso-C15 	601:632	The fatty acids were dominated by branched forms of fatty acids with iso- and anteiso-saturated fatty acids, with a high abundance of anteiso-C15 : 0, anteiso-C17 : 0 and C18 : 0.
30226463	5	4	dep	forms	530:534	arg1	 0					634:635	 0	634:635	 0	634:635	The fatty acids were dominated by branched forms of fatty acids with iso- and anteiso-saturated fatty acids, with a high abundance of anteiso-C15 : 0, anteiso-C17 : 0 and C18 : 0.
30226463	5	4	dep	forms	530:534	arg1	anteiso-C17 					638:649	anteiso-C17 	638:649	anteiso-C17 	638:649	The fatty acids were dominated by branched forms of fatty acids with iso- and anteiso-saturated fatty acids, with a high abundance of anteiso-C15 : 0, anteiso-C17 : 0 and C18 : 0.
30226463	11	5	theme	name	1740:1743	arg1	Salibacterium					1745:1757	the name Salibacterium	1736:1757	the name Salibacterium	1736:1757	Based on its phenotypic characteristics and on phylogenetic inference, strain SMB4T represents a novel species of the genus Salibacterium, for which the name Salibacterium nitratireducens sp.
30226463	5	6	dep	 0	634:635	arg1	 0					663:664	 0	663:664	 0	663:664	The fatty acids were dominated by branched forms of fatty acids with iso- and anteiso-saturated fatty acids, with a high abundance of anteiso-C15 : 0, anteiso-C17 : 0 and C18 : 0.
30226463	5	6	dep	 0	634:635	arg1	C18 					658:661	C18 	658:661	C18 	658:661	The fatty acids were dominated by branched forms of fatty acids with iso- and anteiso-saturated fatty acids, with a high abundance of anteiso-C15 : 0, anteiso-C17 : 0 and C18 : 0.
30226463	5	6	dep	 0	634:635	arg1	 0					651:652	 0	651:652	 0	651:652	The fatty acids were dominated by branched forms of fatty acids with iso- and anteiso-saturated fatty acids, with a high abundance of anteiso-C15 : 0, anteiso-C17 : 0 and C18 : 0.
30226463	6	7	theme	unidentified	855:866	arg1	phospholipid					868:879	one unidentified phospholipid	851:879	one unidentified phospholipid	851:879	The cell-wall peptidoglycan of strain SMB4T contained meso-diaminopimelic acid, while the polar lipids included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unidentified phospholipid and three unidentified lipids.
30226463	8	8	theme	sequence	971:978	arg1	search					991:996	A blast sequence similarity search	963:996	A blast sequence similarity search based on 16S rRNA gene sequence	963:1028	A blast sequence similarity search based on 16S rRNA gene sequence indicated that Salibacterium halochares, Salibacterium halotolerans and Salibacterium qingdaonense were the nearest phylogenetic neighbours, with a pair-wise sequence similarities of 98.4, 98.2 and 97.0 % respectively.
30226463	8	9	theme	nearest	1138:1144	arg1	qingdaonense					1116:1127	Salibacterium qingdaonense	1102:1127	Salibacterium qingdaonense	1102:1127	A blast sequence similarity search based on 16S rRNA gene sequence indicated that Salibacterium halochares, Salibacterium halotolerans and Salibacterium qingdaonense were the nearest phylogenetic neighbours, with a pair-wise sequence similarities of 98.4, 98.2 and 97.0 % respectively.
30226463	8	9	theme	nearest	1138:1144	arg1	halotolerans					1085:1096	Salibacterium halotolerans	1071:1096	Salibacterium halotolerans	1071:1096	A blast sequence similarity search based on 16S rRNA gene sequence indicated that Salibacterium halochares, Salibacterium halotolerans and Salibacterium qingdaonense were the nearest phylogenetic neighbours, with a pair-wise sequence similarities of 98.4, 98.2 and 97.0 % respectively.
30226463	8	9	theme	nearest	1138:1144	arg1	halochares					1059:1068	Salibacterium halochares	1045:1068	Salibacterium halochares	1045:1068	A blast sequence similarity search based on 16S rRNA gene sequence indicated that Salibacterium halochares, Salibacterium halotolerans and Salibacterium qingdaonense were the nearest phylogenetic neighbours, with a pair-wise sequence similarities of 98.4, 98.2 and 97.0 % respectively.
30226463	8	9	theme	nearest	1138:1144	arg1	neighbours					1159:1168	the nearest phylogenetic neighbours	1134:1168	the nearest phylogenetic neighbours	1134:1168	A blast sequence similarity search based on 16S rRNA gene sequence indicated that Salibacterium halochares, Salibacterium halotolerans and Salibacterium qingdaonense were the nearest phylogenetic neighbours, with a pair-wise sequence similarities of 98.4, 98.2 and 97.0 % respectively.
30226463	9	10	theme	strain	1283:1288	arg1	SMB4T					1290:1294	strain SMB4T	1283:1294	strain SMB4T	1283:1294	Phylogenetic analysis showed that strain SMB4T was clustered with S. halochares and together clustered with S. halotolerans and S. qingdaonense.
30226463	5	11	theme	fatty	539:543	arg1	acids					545:549	fatty acids	539:549	fatty acids with iso- and anteiso-saturated fatty acids, with a high abundance of anteiso-C15 	539:632	The fatty acids were dominated by branched forms of fatty acids with iso- and anteiso-saturated fatty acids, with a high abundance of anteiso-C15 : 0, anteiso-C17 : 0 and C18 : 0.
30226463	5	12	theme	iso-	556:559	arg1	acids					589:593	iso- and anteiso-saturated fatty acids	556:593	iso- and anteiso-saturated fatty acids	556:593	The fatty acids were dominated by branched forms of fatty acids with iso- and anteiso-saturated fatty acids, with a high abundance of anteiso-C15 : 0, anteiso-C17 : 0 and C18 : 0.
30226463	2	13	theme	salt	308:311	arg1	lake					313:316	Sambhar salt lake	300:316	Sambhar salt lake	300:316	A strictly aerobic, haloalkali-tolerant, Gram-stain-positive, non-motile, rod-shaped bacterium, designated strain SMB4T, was isolated from a water sample collected from Sambhar salt lake, Rajasthan, India.
30226463	2	13	theme	salt	308:311	arg1	India					330:334	India	330:334	India	330:334	A strictly aerobic, haloalkali-tolerant, Gram-stain-positive, non-motile, rod-shaped bacterium, designated strain SMB4T, was isolated from a water sample collected from Sambhar salt lake, Rajasthan, India.
30226463	2	14	theme	aerobic	142:148	arg1	bacterium					216:224	A strictly aerobic, haloalkali-tolerant, Gram-stain-positive, non-motile, rod-shaped bacterium	131:224	A strictly aerobic, haloalkali-tolerant, Gram-stain-positive, non-motile, rod-shaped bacterium	131:224	A strictly aerobic, haloalkali-tolerant, Gram-stain-positive, non-motile, rod-shaped bacterium, designated strain SMB4T, was isolated from a water sample collected from Sambhar salt lake, Rajasthan, India.
30226463	10	15	theme	SMB4T	1426:1430	arg1	hybridization					1402:1414	DNA-DNA hybridization	1394:1414	DNA-DNA hybridization of strain SMB4T with S. halochares DSM 21373T, S. halotolerans S7T and S. quigdaonense DSM 21621T	1394:1512	DNA-DNA hybridization of strain SMB4T with S. halochares DSM 21373T, S. halotolerans S7T and S. quigdaonense DSM 21621T showed a relatedness values of only 39.8, 26.3 and 42.8 %, respectively.
30226463	5	16	theme	acids	545:549	arg1	forms					530:534	branched forms	521:534	branched forms of fatty acids with iso- and anteiso-saturated fatty acids, with a high abundance of anteiso-C15 : 0, anteiso-C17 : 0 and C18 : 0	521:664	The fatty acids were dominated by branched forms of fatty acids with iso- and anteiso-saturated fatty acids, with a high abundance of anteiso-C15 : 0, anteiso-C17 : 0 and C18 : 0.
30226463	2	17	theme	water	272:276	arg1	sample					278:283	a water sample	270:283	a water sample collected from Sambhar salt lake, Rajasthan, India	270:334	A strictly aerobic, haloalkali-tolerant, Gram-stain-positive, non-motile, rod-shaped bacterium, designated strain SMB4T, was isolated from a water sample collected from Sambhar salt lake, Rajasthan, India.
30226463	3	18	dep	%	371:371	arg1	w/v					374:376	w/v	374:376	w/v	374:376	Growth occurred at 25-50 °C, 4-12 % (w/v) NaCl and pH of 5-9.
30226463	6	19	theme	SMB4T	705:709	arg1	peptidoglycan					681:693	The cell-wall peptidoglycan	667:693	The cell-wall peptidoglycan of strain SMB4T	667:709	The cell-wall peptidoglycan of strain SMB4T contained meso-diaminopimelic acid, while the polar lipids included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unidentified phospholipid and three unidentified lipids.
30226463	13	20	theme	=MTCC	1823:1827	arg1	32187T					1852:1857	=MTCC 12633T=KCTC 33876T=JCM 32187T	1823:1857	=MTCC 12633T=KCTC 33876T=JCM 32187T	1823:1857	The type strain is SMB4T (=MTCC 12633T=KCTC 33876T=JCM 32187T).
30226463	13	20	theme	=MTCC	1823:1827	arg1	SMB4T					1816:1820	SMB4T	1816:1820	SMB4T (=MTCC 12633T=KCTC 33876T=JCM 32187T)	1816:1858	The type strain is SMB4T (=MTCC 12633T=KCTC 33876T=JCM 32187T).
30226463	8	21	theme	phylogenetic	1146:1157	arg1	qingdaonense					1116:1127	Salibacterium qingdaonense	1102:1127	Salibacterium qingdaonense	1102:1127	A blast sequence similarity search based on 16S rRNA gene sequence indicated that Salibacterium halochares, Salibacterium halotolerans and Salibacterium qingdaonense were the nearest phylogenetic neighbours, with a pair-wise sequence similarities of 98.4, 98.2 and 97.0 % respectively.
30226463	8	21	theme	phylogenetic	1146:1157	arg1	halotolerans					1085:1096	Salibacterium halotolerans	1071:1096	Salibacterium halotolerans	1071:1096	A blast sequence similarity search based on 16S rRNA gene sequence indicated that Salibacterium halochares, Salibacterium halotolerans and Salibacterium qingdaonense were the nearest phylogenetic neighbours, with a pair-wise sequence similarities of 98.4, 98.2 and 97.0 % respectively.
30226463	8	21	theme	phylogenetic	1146:1157	arg1	halochares					1059:1068	Salibacterium halochares	1045:1068	Salibacterium halochares	1045:1068	A blast sequence similarity search based on 16S rRNA gene sequence indicated that Salibacterium halochares, Salibacterium halotolerans and Salibacterium qingdaonense were the nearest phylogenetic neighbours, with a pair-wise sequence similarities of 98.4, 98.2 and 97.0 % respectively.
30226463	8	21	theme	phylogenetic	1146:1157	arg1	neighbours					1159:1168	the nearest phylogenetic neighbours	1134:1168	the nearest phylogenetic neighbours	1134:1168	A blast sequence similarity search based on 16S rRNA gene sequence indicated that Salibacterium halochares, Salibacterium halotolerans and Salibacterium qingdaonense were the nearest phylogenetic neighbours, with a pair-wise sequence similarities of 98.4, 98.2 and 97.0 % respectively.
30226463	11	22	theme	Salibacterium	1711:1723	arg1	species					1690:1696	a novel species	1682:1696	a novel species	1682:1696	Based on its phenotypic characteristics and on phylogenetic inference, strain SMB4T represents a novel species of the genus Salibacterium, for which the name Salibacterium nitratireducens sp.
30226463	6	23	theme	strain	698:703	arg1	SMB4T					705:709	strain SMB4T	698:709	strain SMB4T	698:709	The cell-wall peptidoglycan of strain SMB4T contained meso-diaminopimelic acid, while the polar lipids included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unidentified phospholipid and three unidentified lipids.
30226463	10	24	theme	%	1570:1570	arg1	values					1535:1540	a relatedness values	1521:1540	a relatedness values of only 39.8, 26.3 and 42.8 %, respectively	1521:1584	DNA-DNA hybridization of strain SMB4T with S. halochares DSM 21373T, S. halotolerans S7T and S. quigdaonense DSM 21621T showed a relatedness values of only 39.8, 26.3 and 42.8 %, respectively.
30226463	13	25	theme	12633T=KCTC	1829:1839	arg1	32187T					1852:1857	=MTCC 12633T=KCTC 33876T=JCM 32187T	1823:1857	=MTCC 12633T=KCTC 33876T=JCM 32187T	1823:1857	The type strain is SMB4T (=MTCC 12633T=KCTC 33876T=JCM 32187T).
30226463	13	25	theme	12633T=KCTC	1829:1839	arg1	SMB4T					1816:1820	SMB4T	1816:1820	SMB4T (=MTCC 12633T=KCTC 33876T=JCM 32187T)	1816:1858	The type strain is SMB4T (=MTCC 12633T=KCTC 33876T=JCM 32187T).
30226463	10	26	with	hybridization	1402:1414	arg1	21373T					1455:1460	S. halochares DSM 21373T	1437:1460	S. halochares DSM 21373T	1437:1460	DNA-DNA hybridization of strain SMB4T with S. halochares DSM 21373T, S. halotolerans S7T and S. quigdaonense DSM 21621T showed a relatedness values of only 39.8, 26.3 and 42.8 %, respectively.
30226463	10	26	with	hybridization	1402:1414	arg1	21621T					1507:1512	S. quigdaonense DSM 21621T	1487:1512	S. quigdaonense DSM 21621T	1487:1512	DNA-DNA hybridization of strain SMB4T with S. halochares DSM 21373T, S. halotolerans S7T and S. quigdaonense DSM 21621T showed a relatedness values of only 39.8, 26.3 and 42.8 %, respectively.
30226463	10	26	with	hybridization	1402:1414	arg1	S7T					1479:1481	S7T	1479:1481	S7T	1479:1481	DNA-DNA hybridization of strain SMB4T with S. halochares DSM 21373T, S. halotolerans S7T and S. quigdaonense DSM 21621T showed a relatedness values of only 39.8, 26.3 and 42.8 %, respectively.
30226463	10	27	theme	strain	1419:1424	arg1	SMB4T					1426:1430	strain SMB4T	1419:1430	strain SMB4T	1419:1430	DNA-DNA hybridization of strain SMB4T with S. halochares DSM 21373T, S. halotolerans S7T and S. quigdaonense DSM 21621T showed a relatedness values of only 39.8, 26.3 and 42.8 %, respectively.
30226463	5	28	theme	anteiso-saturated	565:581	arg1	acids					589:593	iso- and anteiso-saturated fatty acids	556:593	iso- and anteiso-saturated fatty acids	556:593	The fatty acids were dominated by branched forms of fatty acids with iso- and anteiso-saturated fatty acids, with a high abundance of anteiso-C15 : 0, anteiso-C17 : 0 and C18 : 0.
30226463	1	29	from	India	124:128	arg1	sample					93:98	a water sample	85:98	a water sample from Sambhar salt lake, India	85:128	nov., a haloalkalitolerant bacterium isolated from a water sample from Sambhar salt lake, India.
30226463	1	30	theme	water	87:91	arg1	sample					93:98	a water sample	85:98	a water sample from Sambhar salt lake, India	85:128	nov., a haloalkalitolerant bacterium isolated from a water sample from Sambhar salt lake, India.
30226463	13	31	theme	33876T=JCM	1841:1850	arg1	32187T					1852:1857	=MTCC 12633T=KCTC 33876T=JCM 32187T	1823:1857	=MTCC 12633T=KCTC 33876T=JCM 32187T	1823:1857	The type strain is SMB4T (=MTCC 12633T=KCTC 33876T=JCM 32187T).
30226463	13	31	theme	33876T=JCM	1841:1850	arg1	SMB4T					1816:1820	SMB4T	1816:1820	SMB4T (=MTCC 12633T=KCTC 33876T=JCM 32187T)	1816:1858	The type strain is SMB4T (=MTCC 12633T=KCTC 33876T=JCM 32187T).
30226463	8	32	theme	97.0 	1228:1232	arg1	%					1233:1233	98.4, 98.2 and 97.0 %	1213:1233	%	1233:1233	A blast sequence similarity search based on 16S rRNA gene sequence indicated that Salibacterium halochares, Salibacterium halotolerans and Salibacterium qingdaonense were the nearest phylogenetic neighbours, with a pair-wise sequence similarities of 98.4, 98.2 and 97.0 % respectively.
30226463	3	33	theme	4-12 	366:370	arg1	%					371:371	%	371:371	%	371:371	Growth occurred at 25-50 °C, 4-12 % (w/v) NaCl and pH of 5-9.
30226463	1	34	attach	isolated	71:78	arg2	bacterium					61:69	a haloalkalitolerant bacterium	40:69	a haloalkalitolerant bacterium isolated from a water sample from Sambhar salt lake, India	40:128	nov., a haloalkalitolerant bacterium isolated from a water sample from Sambhar salt lake, India.
30226463	1	34	attach	isolated	71:78	arg1	sample					93:98	a water sample	85:98	a water sample from Sambhar salt lake, India	85:128	nov., a haloalkalitolerant bacterium isolated from a water sample from Sambhar salt lake, India.
30226463	5	35	theme	fatty	583:587	arg1	acids					589:593	iso- and anteiso-saturated fatty acids	556:593	iso- and anteiso-saturated fatty acids	556:593	The fatty acids were dominated by branched forms of fatty acids with iso- and anteiso-saturated fatty acids, with a high abundance of anteiso-C15 : 0, anteiso-C17 : 0 and C18 : 0.
30226463	5	36	theme	branched	521:528	arg1	forms					530:534	branched forms	521:534	branched forms of fatty acids with iso- and anteiso-saturated fatty acids, with a high abundance of anteiso-C15 : 0, anteiso-C17 : 0 and C18 : 0	521:664	The fatty acids were dominated by branched forms of fatty acids with iso- and anteiso-saturated fatty acids, with a high abundance of anteiso-C15 : 0, anteiso-C17 : 0 and C18 : 0.
30226463	2	37	theme	Sambhar	300:306	arg1	lake					313:316	Sambhar salt lake	300:316	Sambhar salt lake	300:316	A strictly aerobic, haloalkali-tolerant, Gram-stain-positive, non-motile, rod-shaped bacterium, designated strain SMB4T, was isolated from a water sample collected from Sambhar salt lake, Rajasthan, India.
30226463	2	37	theme	Sambhar	300:306	arg1	India					330:334	India	330:334	India	330:334	A strictly aerobic, haloalkali-tolerant, Gram-stain-positive, non-motile, rod-shaped bacterium, designated strain SMB4T, was isolated from a water sample collected from Sambhar salt lake, Rajasthan, India.
30226463	13	38	theme	type	1801:1804	arg1	SMB4T					1816:1820	SMB4T	1816:1820	SMB4T (=MTCC 12633T=KCTC 33876T=JCM 32187T)	1816:1858	The type strain is SMB4T (=MTCC 12633T=KCTC 33876T=JCM 32187T).
30226463	13	38	theme	type	1801:1804	arg1	strain					1806:1811	The type strain	1797:1811	The type strain	1797:1811	The type strain is SMB4T (=MTCC 12633T=KCTC 33876T=JCM 32187T).
30226463	10	39	theme	S.	1437:1438	arg1	21373T					1455:1460	S. halochares DSM 21373T	1437:1460	S. halochares DSM 21373T	1437:1460	DNA-DNA hybridization of strain SMB4T with S. halochares DSM 21373T, S. halotolerans S7T and S. quigdaonense DSM 21621T showed a relatedness values of only 39.8, 26.3 and 42.8 %, respectively.
30226463	8	40	theme	pair-wise	1178:1186	arg1	similarities					1197:1208	a pair-wise sequence similarities	1176:1208	a pair-wise sequence similarities of 98.4, 98.2 and 97.0 % respectively	1176:1246	A blast sequence similarity search based on 16S rRNA gene sequence indicated that Salibacterium halochares, Salibacterium halotolerans and Salibacterium qingdaonense were the nearest phylogenetic neighbours, with a pair-wise sequence similarities of 98.4, 98.2 and 97.0 % respectively.
30226463	8	41	theme	blast	965:969	arg1	search					991:996	A blast sequence similarity search	963:996	A blast sequence similarity search based on 16S rRNA gene sequence	963:1028	A blast sequence similarity search based on 16S rRNA gene sequence indicated that Salibacterium halochares, Salibacterium halotolerans and Salibacterium qingdaonense were the nearest phylogenetic neighbours, with a pair-wise sequence similarities of 98.4, 98.2 and 97.0 % respectively.
30226463	10	42	theme	S.	1487:1488	arg1	21621T					1507:1512	S. quigdaonense DSM 21621T	1487:1512	S. quigdaonense DSM 21621T	1487:1512	DNA-DNA hybridization of strain SMB4T with S. halochares DSM 21373T, S. halotolerans S7T and S. quigdaonense DSM 21621T showed a relatedness values of only 39.8, 26.3 and 42.8 %, respectively.
30226463	6	43	theme	cell-wall	671:679	arg1	peptidoglycan					681:693	The cell-wall peptidoglycan	667:693	The cell-wall peptidoglycan of strain SMB4T	667:709	The cell-wall peptidoglycan of strain SMB4T contained meso-diaminopimelic acid, while the polar lipids included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unidentified phospholipid and three unidentified lipids.
30226463	10	44	theme	DSM	1451:1453	arg1	21373T					1455:1460	S. halochares DSM 21373T	1437:1460	S. halochares DSM 21373T	1437:1460	DNA-DNA hybridization of strain SMB4T with S. halochares DSM 21373T, S. halotolerans S7T and S. quigdaonense DSM 21621T showed a relatedness values of only 39.8, 26.3 and 42.8 %, respectively.
30226463	2	45	dep	aerobic	142:148	arg1	non-motile					193:202	non-motile	193:202	non-motile	193:202	A strictly aerobic, haloalkali-tolerant, Gram-stain-positive, non-motile, rod-shaped bacterium, designated strain SMB4T, was isolated from a water sample collected from Sambhar salt lake, Rajasthan, India.
30226463	2	45	dep	aerobic	142:148	arg1	Gram-stain-positive					172:190	Gram-stain-positive	172:190	Gram-stain-positive	172:190	A strictly aerobic, haloalkali-tolerant, Gram-stain-positive, non-motile, rod-shaped bacterium, designated strain SMB4T, was isolated from a water sample collected from Sambhar salt lake, Rajasthan, India.
30226463	2	45	dep	aerobic	142:148	arg1	haloalkali-tolerant					151:169	haloalkali-tolerant	151:169	haloalkali-tolerant	151:169	A strictly aerobic, haloalkali-tolerant, Gram-stain-positive, non-motile, rod-shaped bacterium, designated strain SMB4T, was isolated from a water sample collected from Sambhar salt lake, Rajasthan, India.
30226463	2	45	dep	aerobic	142:148	arg1	rod-shaped					205:214	rod-shaped	205:214	rod-shaped	205:214	A strictly aerobic, haloalkali-tolerant, Gram-stain-positive, non-motile, rod-shaped bacterium, designated strain SMB4T, was isolated from a water sample collected from Sambhar salt lake, Rajasthan, India.
30226463	7	46	theme	DNA	916:918	arg1	G+C content					920:930	The DNA G+C content	912:930	The DNA G+C content of strain SMB4T	912:946	The DNA G+C content of strain SMB4T was 49.1 mol%.
30226463	7	46	theme	DNA	916:918	arg1	%					960:960	49.1 mol%	952:960	49.1 mol%	952:960	The DNA G+C content of strain SMB4T was 49.1 mol%.
30226463	8	47	theme	%	1233:1233	arg1	similarities					1197:1208	a pair-wise sequence similarities	1176:1208	a pair-wise sequence similarities of 98.4, 98.2 and 97.0 % respectively	1176:1246	A blast sequence similarity search based on 16S rRNA gene sequence indicated that Salibacterium halochares, Salibacterium halotolerans and Salibacterium qingdaonense were the nearest phylogenetic neighbours, with a pair-wise sequence similarities of 98.4, 98.2 and 97.0 % respectively.
30226463	8	48	theme	16S	1007:1009	arg1	rRNA					1011:1014	16S rRNA	1007:1014	16S rRNA gene sequence	1007:1028	A blast sequence similarity search based on 16S rRNA gene sequence indicated that Salibacterium halochares, Salibacterium halotolerans and Salibacterium qingdaonense were the nearest phylogenetic neighbours, with a pair-wise sequence similarities of 98.4, 98.2 and 97.0 % respectively.
30226463	2	49	theme	strain	238:243	arg1	SMB4T					245:249	strain SMB4T	238:249	strain SMB4T	238:249	A strictly aerobic, haloalkali-tolerant, Gram-stain-positive, non-motile, rod-shaped bacterium, designated strain SMB4T, was isolated from a water sample collected from Sambhar salt lake, Rajasthan, India.
30226463	3	50	theme	5-9	394:396	arg1	pH					388:389	pH	388:389	pH of 5-9	388:396	Growth occurred at 25-50 °C, 4-12 % (w/v) NaCl and pH of 5-9.
30226463	3	50	theme	5-9	394:396	arg1	NaCl					379:382	4-12 % (w/v) NaCl	366:382	4-12 % (w/v) NaCl	366:382	Growth occurred at 25-50 °C, 4-12 % (w/v) NaCl and pH of 5-9.
30226463	3	50	theme	5-9	394:396	arg1	25-50 °C					356:363	25-50 °C	356:363	25-50 °C	356:363	Growth occurred at 25-50 °C, 4-12 % (w/v) NaCl and pH of 5-9.
30226463	8	51	theme	sequence	1188:1195	arg1	similarities					1197:1208	a pair-wise sequence similarities	1176:1208	a pair-wise sequence similarities of 98.4, 98.2 and 97.0 % respectively	1176:1246	A blast sequence similarity search based on 16S rRNA gene sequence indicated that Salibacterium halochares, Salibacterium halotolerans and Salibacterium qingdaonense were the nearest phylogenetic neighbours, with a pair-wise sequence similarities of 98.4, 98.2 and 97.0 % respectively.
30226463	4	52	theme	urease	468:473	arg1	activities					475:484	urease activities	468:484	urease activities	468:484	Strain SMB4T was positive for β-galactosidase, oxidase, catalase and urease activities.
30226463	11	53	theme	strain	1658:1663	arg1	SMB4T					1665:1669	strain SMB4T	1658:1669	strain SMB4T	1658:1669	Based on its phenotypic characteristics and on phylogenetic inference, strain SMB4T represents a novel species of the genus Salibacterium, for which the name Salibacterium nitratireducens sp.
30226463	3	54	theme	%	371:371	arg1	NaCl					379:382	4-12 % (w/v) NaCl	366:382	4-12 % (w/v) NaCl	366:382	Growth occurred at 25-50 °C, 4-12 % (w/v) NaCl and pH of 5-9.
30226463	5	55	with	acids	545:549	arg1	acids					589:593	iso- and anteiso-saturated fatty acids	556:593	iso- and anteiso-saturated fatty acids	556:593	The fatty acids were dominated by branched forms of fatty acids with iso- and anteiso-saturated fatty acids, with a high abundance of anteiso-C15 : 0, anteiso-C17 : 0 and C18 : 0.
30226463	5	56	theme	high	603:606	arg1	abundance					608:616	a high abundance	601:616	a high abundance of anteiso-C15 	601:632	The fatty acids were dominated by branched forms of fatty acids with iso- and anteiso-saturated fatty acids, with a high abundance of anteiso-C15 : 0, anteiso-C17 : 0 and C18 : 0.
30226463	4	57	theme	Strain	399:404	arg1	SMB4T					406:410	Strain SMB4T	399:410	Strain SMB4T	399:410	Strain SMB4T was positive for β-galactosidase, oxidase, catalase and urease activities.
30226463	5	58	theme	fatty	491:495	arg1	acids					497:501	The fatty acids	487:501	The fatty acids	487:501	The fatty acids were dominated by branched forms of fatty acids with iso- and anteiso-saturated fatty acids, with a high abundance of anteiso-C15 : 0, anteiso-C17 : 0 and C18 : 0.
30226463	11	59	theme	phenotypic	1600:1609	arg1	characteristics					1611:1625	its phenotypic characteristics	1596:1625	its phenotypic characteristics	1596:1625	Based on its phenotypic characteristics and on phylogenetic inference, strain SMB4T represents a novel species of the genus Salibacterium, for which the name Salibacterium nitratireducens sp.
30226463	10	60	dep	S.	1437:1438	arg1	halochares					1440:1449	halochares	1440:1449	halochares	1440:1449	DNA-DNA hybridization of strain SMB4T with S. halochares DSM 21373T, S. halotolerans S7T and S. quigdaonense DSM 21621T showed a relatedness values of only 39.8, 26.3 and 42.8 %, respectively.
30226463	6	61	theme	unidentified	891:902	arg1	lipids					904:909	three unidentified lipids	885:909	three unidentified lipids	885:909	The cell-wall peptidoglycan of strain SMB4T contained meso-diaminopimelic acid, while the polar lipids included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unidentified phospholipid and three unidentified lipids.
30226463	6	62	theme	polar	757:761	arg1	lipids					763:768	the polar lipids	753:768	the polar lipids	753:768	The cell-wall peptidoglycan of strain SMB4T contained meso-diaminopimelic acid, while the polar lipids included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unidentified phospholipid and three unidentified lipids.
30226463	11	63	theme	phylogenetic	1634:1645	arg1	inference					1647:1655	phylogenetic inference	1634:1655	phylogenetic inference	1634:1655	Based on its phenotypic characteristics and on phylogenetic inference, strain SMB4T represents a novel species of the genus Salibacterium, for which the name Salibacterium nitratireducens sp.
30226463	8	64	theme	gene	1016:1019	arg1	sequence					1021:1028	16S rRNA gene sequence	1007:1028	16S rRNA gene sequence	1007:1028	A blast sequence similarity search based on 16S rRNA gene sequence indicated that Salibacterium halochares, Salibacterium halotolerans and Salibacterium qingdaonense were the nearest phylogenetic neighbours, with a pair-wise sequence similarities of 98.4, 98.2 and 97.0 % respectively.
30226463	10	65	theme	DNA-DNA	1394:1400	arg1	hybridization					1402:1414	DNA-DNA hybridization	1394:1414	DNA-DNA hybridization of strain SMB4T with S. halochares DSM 21373T, S. halotolerans S7T and S. quigdaonense DSM 21621T	1394:1512	DNA-DNA hybridization of strain SMB4T with S. halochares DSM 21373T, S. halotolerans S7T and S. quigdaonense DSM 21621T showed a relatedness values of only 39.8, 26.3 and 42.8 %, respectively.
30226463	10	66	theme	DSM	1503:1505	arg1	21621T					1507:1512	S. quigdaonense DSM 21621T	1487:1512	S. quigdaonense DSM 21621T	1487:1512	DNA-DNA hybridization of strain SMB4T with S. halochares DSM 21373T, S. halotolerans S7T and S. quigdaonense DSM 21621T showed a relatedness values of only 39.8, 26.3 and 42.8 %, respectively.
30226463	8	67	with	neighbours	1159:1168	arg1	similarities					1197:1208	a pair-wise sequence similarities	1176:1208	a pair-wise sequence similarities of 98.4, 98.2 and 97.0 % respectively	1176:1246	A blast sequence similarity search based on 16S rRNA gene sequence indicated that Salibacterium halochares, Salibacterium halotolerans and Salibacterium qingdaonense were the nearest phylogenetic neighbours, with a pair-wise sequence similarities of 98.4, 98.2 and 97.0 % respectively.
30226463	6	68	theme	meso-diaminopimelic	721:739	arg1	acid					741:744	meso-diaminopimelic acid	721:744	meso-diaminopimelic acid	721:744	The cell-wall peptidoglycan of strain SMB4T contained meso-diaminopimelic acid, while the polar lipids included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unidentified phospholipid and three unidentified lipids.
30226463	1	69	dep	bacterium	61:69	arg1	nov.					34:37	nov.	34:37	nov.	34:37	nov., a haloalkalitolerant bacterium isolated from a water sample from Sambhar salt lake, India.
30226463	5	70	theme	anteiso-C15 	621:632	arg1	abundance					608:616	a high abundance	601:616	a high abundance of anteiso-C15 	601:632	The fatty acids were dominated by branched forms of fatty acids with iso- and anteiso-saturated fatty acids, with a high abundance of anteiso-C15 : 0, anteiso-C17 : 0 and C18 : 0.
30226463	11	71	theme	novel	1684:1688	arg1	species					1690:1696	a novel species	1682:1696	a novel species	1682:1696	Based on its phenotypic characteristics and on phylogenetic inference, strain SMB4T represents a novel species of the genus Salibacterium, for which the name Salibacterium nitratireducens sp.
30226463	8	72	theme	98.4	1213:1216	arg1	%					1233:1233	98.4, 98.2 and 97.0 %	1213:1233	%	1233:1233	A blast sequence similarity search based on 16S rRNA gene sequence indicated that Salibacterium halochares, Salibacterium halotolerans and Salibacterium qingdaonense were the nearest phylogenetic neighbours, with a pair-wise sequence similarities of 98.4, 98.2 and 97.0 % respectively.
30226463	2	73	attach	isolated	256:263	arg2	bacterium					216:224	A strictly aerobic, haloalkali-tolerant, Gram-stain-positive, non-motile, rod-shaped bacterium	131:224	A strictly aerobic, haloalkali-tolerant, Gram-stain-positive, non-motile, rod-shaped bacterium	131:224	A strictly aerobic, haloalkali-tolerant, Gram-stain-positive, non-motile, rod-shaped bacterium, designated strain SMB4T, was isolated from a water sample collected from Sambhar salt lake, Rajasthan, India.
30226463	2	73	attach	isolated	256:263	arg1	sample					278:283	a water sample	270:283	a water sample collected from Sambhar salt lake, Rajasthan, India	270:334	A strictly aerobic, haloalkali-tolerant, Gram-stain-positive, non-motile, rod-shaped bacterium, designated strain SMB4T, was isolated from a water sample collected from Sambhar salt lake, Rajasthan, India.
30226463	8	74	theme	rRNA	1011:1014	arg1	sequence					1021:1028	16S rRNA gene sequence	1007:1028	16S rRNA gene sequence	1007:1028	A blast sequence similarity search based on 16S rRNA gene sequence indicated that Salibacterium halochares, Salibacterium halotolerans and Salibacterium qingdaonense were the nearest phylogenetic neighbours, with a pair-wise sequence similarities of 98.4, 98.2 and 97.0 % respectively.
30226463	9	75	theme	Phylogenetic	1249:1260	arg1	analysis					1262:1269	Phylogenetic analysis	1249:1269	Phylogenetic analysis	1249:1269	Phylogenetic analysis showed that strain SMB4T was clustered with S. halochares and together clustered with S. halotolerans and S. qingdaonense.
30226463	7	76	theme	49.1 mol	952:959	arg1	G+C content					920:930	The DNA G+C content	912:930	The DNA G+C content of strain SMB4T	912:946	The DNA G+C content of strain SMB4T was 49.1 mol%.
30226463	7	76	theme	49.1 mol	952:959	arg1	%					960:960	49.1 mol%	952:960	49.1 mol%	952:960	The DNA G+C content of strain SMB4T was 49.1 mol%.
30226463	11	77	theme	genus	1705:1709	arg1	Salibacterium					1711:1723	the genus Salibacterium	1701:1723	the genus Salibacterium	1701:1723	Based on its phenotypic characteristics and on phylogenetic inference, strain SMB4T represents a novel species of the genus Salibacterium, for which the name Salibacterium nitratireducens sp.
30226463	6	78	contain	contained	711:719	arg2	acid					741:744	meso-diaminopimelic acid	721:744	meso-diaminopimelic acid	721:744	The cell-wall peptidoglycan of strain SMB4T contained meso-diaminopimelic acid, while the polar lipids included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unidentified phospholipid and three unidentified lipids.
30226463	6	78	contain	contained	711:719	arg1	peptidoglycan					681:693	The cell-wall peptidoglycan	667:693	The cell-wall peptidoglycan of strain SMB4T	667:709	The cell-wall peptidoglycan of strain SMB4T contained meso-diaminopimelic acid, while the polar lipids included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unidentified phospholipid and three unidentified lipids.
30226463	1	79	theme	haloalkalitolerant	42:59	arg1	bacterium					61:69	a haloalkalitolerant bacterium	40:69	a haloalkalitolerant bacterium isolated from a water sample from Sambhar salt lake, India	40:128	nov., a haloalkalitolerant bacterium isolated from a water sample from Sambhar salt lake, India.
30226463	7	80	theme	strain	935:940	arg1	SMB4T					942:946	strain SMB4T	935:946	strain SMB4T	935:946	The DNA G+C content of strain SMB4T was 49.1 mol%.
30226463	10	81	theme	quigdaonense	1490:1501	arg1	21621T					1507:1512	S. quigdaonense DSM 21621T	1487:1512	S. quigdaonense DSM 21621T	1487:1512	DNA-DNA hybridization of strain SMB4T with S. halochares DSM 21373T, S. halotolerans S7T and S. quigdaonense DSM 21621T showed a relatedness values of only 39.8, 26.3 and 42.8 %, respectively.
29701575	4	0	theme	coherent	541:548	arg1	branch					550:555	a coherent branch	539:555	a coherent branch with P. vulneris CCUG 53270T and Paenibacillus yunnanensis YN2T	539:619	Phylogenetic analysis demonstrated that the novel candidate formed a coherent branch with P. vulneris CCUG 53270T and Paenibacillus yunnanensis YN2T.
29701575	2	1	theme	peritrichous	102:113	arg1	flagella					115:122	peritrichous flagella	102:122	peritrichous flagella	102:122	A Gram-stain-positive, aerobic, motile with peritrichous flagella, rod-shaped bacterium, designated CFH S0170T, was isolated from a soil sample collected from Catba island in Ha Long Bay, Hai Phong City, Vietnam.
29701575	15	2	theme	type	1628:1631	arg1	S0170T					1647:1652	CFH S0170T	1643:1652	CFH S0170T (=KCTC 33624T=BCRC 80802T)	1643:1679	The type strain is CFH S0170T (=KCTC 33624T=BCRC 80802T).
29701575	15	2	theme	type	1628:1631	arg1	strain					1633:1638	The type strain	1624:1638	The type strain	1624:1638	The type strain is CFH S0170T (=KCTC 33624T=BCRC 80802T).
29701575	12	3	theme	other	1403:1407	arg1	species					1415:1421	other valid species	1403:1421	other valid species of this genus	1403:1435	On the basis of phenotypic, chemotaxonomic and phylogenetic analysis, strain CFH S0170T is affiliated to the genus Paenibacillus, but could be distinguished from other valid species of this genus.
29701575	5	4	with	similarity	685:694	arg1	53270T					718:723	P. vulneris CCUG 53270T	701:723	P. vulneris CCUG 53270T	701:723	Furthermore, the novel strain shared 87.2 % rpoB gene sequence similarity with P. vulneris CCUG 53270T.
29701575	12	5	theme	Paenibacillus	1356:1368	arg1	genus					1350:1354	the genus Paenibacillus	1346:1368	the genus Paenibacillus	1346:1368	On the basis of phenotypic, chemotaxonomic and phylogenetic analysis, strain CFH S0170T is affiliated to the genus Paenibacillus, but could be distinguished from other valid species of this genus.
29701575	6	6	theme	S0170T	747:752	arg1	Growth					726:731	Growth	726:731	Growth of strain CFH S0170T	726:752	Growth of strain CFH S0170T occurred at 10-40 °C, pH 6.0-8.0 and with 0-2.0 % (w/v) NaCl.
29701575	13	7	theme	Paenibacillus	1540:1552	arg1	genus					1534:1538	the genus Paenibacillus	1530:1552	the genus Paenibacillus	1530:1552	It is concluded that strain CFH S0170T should be considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus esterisolvens sp.
29701575	2	8	theme	Gram-stain-positive	60:78	arg1	bacterium					136:144	A Gram-stain-positive, aerobic, motile with peritrichous flagella, rod-shaped bacterium	58:144	A Gram-stain-positive, aerobic, motile with peritrichous flagella, rod-shaped bacterium	58:144	A Gram-stain-positive, aerobic, motile with peritrichous flagella, rod-shaped bacterium, designated CFH S0170T, was isolated from a soil sample collected from Catba island in Ha Long Bay, Hai Phong City, Vietnam.
29701575	6	9	theme	strain	736:741	arg1	S0170T					747:752	strain CFH S0170T	736:752	strain CFH S0170T	736:752	Growth of strain CFH S0170T occurred at 10-40 °C, pH 6.0-8.0 and with 0-2.0 % (w/v) NaCl.
29701575	8	10	theme	meso-diaminopimelic	944:962	arg1	acid					964:967	meso-diaminopimelic acid	944:967	meso-diaminopimelic acid	944:967	The cell-wall peptidoglycan contained meso-diaminopimelic acid, glutamic acid, lysine and aspartic acid.
29701575	4	11	theme	Paenibacillus	590:602	arg1	YN2T					616:619	Paenibacillus yunnanensis YN2T	590:619	Paenibacillus yunnanensis YN2T	590:619	Phylogenetic analysis demonstrated that the novel candidate formed a coherent branch with P. vulneris CCUG 53270T and Paenibacillus yunnanensis YN2T.
29701575	5	12	theme	rpoB	666:669	arg1	similarity					685:694	87.2 % rpoB gene sequence similarity	659:694	87.2 % rpoB gene sequence similarity with P. vulneris CCUG 53270T	659:723	Furthermore, the novel strain shared 87.2 % rpoB gene sequence similarity with P. vulneris CCUG 53270T.
29701575	5	13	theme	sequence	676:683	arg1	similarity					685:694	87.2 % rpoB gene sequence similarity	659:694	87.2 % rpoB gene sequence similarity with P. vulneris CCUG 53270T	659:723	Furthermore, the novel strain shared 87.2 % rpoB gene sequence similarity with P. vulneris CCUG 53270T.
29701575	12	14	theme	strain	1311:1316	arg1	S0170T					1322:1327	strain CFH S0170T	1311:1327	strain CFH S0170T	1311:1327	On the basis of phenotypic, chemotaxonomic and phylogenetic analysis, strain CFH S0170T is affiliated to the genus Paenibacillus, but could be distinguished from other valid species of this genus.
29701575	5	15	theme	87.2 	659:663	arg1	%					664:664	%	664:664	%	664:664	Furthermore, the novel strain shared 87.2 % rpoB gene sequence similarity with P. vulneris CCUG 53270T.
29701575	4	16	with	branch	550:555	arg1	YN2T					616:619	Paenibacillus yunnanensis YN2T	590:619	Paenibacillus yunnanensis YN2T	590:619	Phylogenetic analysis demonstrated that the novel candidate formed a coherent branch with P. vulneris CCUG 53270T and Paenibacillus yunnanensis YN2T.
29701575	4	16	with	branch	550:555	arg1	53270T					579:584	P. vulneris CCUG 53270T	562:584	P. vulneris CCUG 53270T	562:584	Phylogenetic analysis demonstrated that the novel candidate formed a coherent branch with P. vulneris CCUG 53270T and Paenibacillus yunnanensis YN2T.
29701575	2	17	with	motile	90:95	arg1	flagella					115:122	peritrichous flagella	102:122	peritrichous flagella	102:122	A Gram-stain-positive, aerobic, motile with peritrichous flagella, rod-shaped bacterium, designated CFH S0170T, was isolated from a soil sample collected from Catba island in Ha Long Bay, Hai Phong City, Vietnam.
29701575	15	18	theme	33624T=BCRC	1661:1671	arg1	S0170T					1647:1652	CFH S0170T	1643:1652	CFH S0170T (=KCTC 33624T=BCRC 80802T)	1643:1679	The type strain is CFH S0170T (=KCTC 33624T=BCRC 80802T).
29701575	15	18	theme	33624T=BCRC	1661:1671	arg1	80802T					1673:1678	=KCTC 33624T=BCRC 80802T	1655:1678	=KCTC 33624T=BCRC 80802T	1655:1678	The type strain is CFH S0170T (=KCTC 33624T=BCRC 80802T).
29701575	7	19	theme	major	881:885	arg1	sugars					898:903	the major whole-cell sugars	877:903	the major whole-cell sugars	877:903	Strain CFH S0170T contained mannose, glucose and rhamnose as the major whole-cell sugars.
29701575	7	19	theme	major	881:885	arg1	rhamnose					865:872	rhamnose	865:872	rhamnose	865:872	Strain CFH S0170T contained mannose, glucose and rhamnose as the major whole-cell sugars.
29701575	7	19	theme	major	881:885	arg1	glucose					853:859	glucose	853:859	glucose	853:859	Strain CFH S0170T contained mannose, glucose and rhamnose as the major whole-cell sugars.
29701575	7	19	theme	major	881:885	arg1	mannose					844:850	mannose	844:850	mannose	844:850	Strain CFH S0170T contained mannose, glucose and rhamnose as the major whole-cell sugars.
29701575	3	20	theme	sequences	303:311	arg1	Comparison					271:280	Comparison	271:280	Comparison of the 16S rRNA gene sequences	271:311	Comparison of the 16S rRNA gene sequences showed that strain CFH S0170T belonged to the genus Paenibacillus and showed closest relationship with Paenibacillus vulneris CCUG 53270T (98.1 % similarity).
29701575	13	21	theme	strain	1459:1464	arg1	S0170T					1470:1475	strain CFH S0170T	1459:1475	strain CFH S0170T	1459:1475	It is concluded that strain CFH S0170T should be considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus esterisolvens sp.
29701575	15	22	theme	CFH	1643:1645	arg1	S0170T					1647:1652	CFH S0170T	1643:1652	CFH S0170T (=KCTC 33624T=BCRC 80802T)	1643:1679	The type strain is CFH S0170T (=KCTC 33624T=BCRC 80802T).
29701575	15	22	theme	CFH	1643:1645	arg1	80802T					1673:1678	=KCTC 33624T=BCRC 80802T	1655:1678	=KCTC 33624T=BCRC 80802T	1655:1678	The type strain is CFH S0170T (=KCTC 33624T=BCRC 80802T).
29701575	15	22	theme	CFH	1643:1645	arg1	strain					1633:1638	The type strain	1624:1638	The type strain	1624:1638	The type strain is CFH S0170T (=KCTC 33624T=BCRC 80802T).
29701575	3	23	theme	rRNA	293:296	arg1	sequences					303:311	the 16S rRNA gene sequences	285:311	the 16S rRNA gene sequences	285:311	Comparison of the 16S rRNA gene sequences showed that strain CFH S0170T belonged to the genus Paenibacillus and showed closest relationship with Paenibacillus vulneris CCUG 53270T (98.1 % similarity).
29701575	5	24	theme	vulneris	704:711	arg1	53270T					718:723	P. vulneris CCUG 53270T	701:723	P. vulneris CCUG 53270T	701:723	Furthermore, the novel strain shared 87.2 % rpoB gene sequence similarity with P. vulneris CCUG 53270T.
29701575	9	25	contain	contained	1035:1043	arg2	phosphatidylethanolamine					1069:1092	phosphatidylethanolamine	1069:1092	phosphatidylethanolamine	1069:1092	The polar lipid profile contained diphosphatidylglycerol, phosphatidylethanolamine, glycolipids and phospholipids.
29701575	9	25	contain	contained	1035:1043	arg2	diphosphatidylglycerol					1045:1066	diphosphatidylglycerol	1045:1066	diphosphatidylglycerol	1045:1066	The polar lipid profile contained diphosphatidylglycerol, phosphatidylethanolamine, glycolipids and phospholipids.
29701575	9	25	contain	contained	1035:1043	arg2	phospholipids					1111:1123	phospholipids	1111:1123	phospholipids	1111:1123	The polar lipid profile contained diphosphatidylglycerol, phosphatidylethanolamine, glycolipids and phospholipids.
29701575	9	25	contain	contained	1035:1043	arg1	profile					1027:1033	The polar lipid profile	1011:1033	The polar lipid profile	1011:1033	The polar lipid profile contained diphosphatidylglycerol, phosphatidylethanolamine, glycolipids and phospholipids.
29701575	9	25	contain	contained	1035:1043	arg2	glycolipids					1095:1105	glycolipids	1095:1105	glycolipids	1095:1105	The polar lipid profile contained diphosphatidylglycerol, phosphatidylethanolamine, glycolipids and phospholipids.
29701575	3	26	theme	CFH	332:334	arg1	S0170T					336:341	strain CFH S0170T	325:341	strain CFH S0170T	325:341	Comparison of the 16S rRNA gene sequences showed that strain CFH S0170T belonged to the genus Paenibacillus and showed closest relationship with Paenibacillus vulneris CCUG 53270T (98.1 % similarity).
29701575	9	27	theme	lipid	1021:1025	arg1	profile					1027:1033	The polar lipid profile	1011:1033	The polar lipid profile	1011:1033	The polar lipid profile contained diphosphatidylglycerol, phosphatidylethanolamine, glycolipids and phospholipids.
29701575	3	28	theme	Paenibacillus	365:377	arg1	genus					359:363	the genus Paenibacillus	355:377	the genus Paenibacillus	355:377	Comparison of the 16S rRNA gene sequences showed that strain CFH S0170T belonged to the genus Paenibacillus and showed closest relationship with Paenibacillus vulneris CCUG 53270T (98.1 % similarity).
29701575	0	29	theme	Paenibacillus	0:12	arg1	esterisolvens					14:26	Paenibacillus esterisolvens	0:26	Paenibacillus esterisolvens	0:26	Paenibacillus esterisolvens sp.
29701575	8	30	theme	aspartic	996:1003	arg1	acid					1005:1008	aspartic acid	996:1008	aspartic acid	996:1008	The cell-wall peptidoglycan contained meso-diaminopimelic acid, glutamic acid, lysine and aspartic acid.
29701575	13	31	theme	name	1569:1572	arg1	esterisolvens					1588:1600	the name Paenibacillus esterisolvens	1565:1600	the name Paenibacillus esterisolvens	1565:1600	It is concluded that strain CFH S0170T should be considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus esterisolvens sp.
29701575	3	32	theme	Paenibacillus	416:428	arg1	similarity					459:468	98.1 % similarity	452:468	98.1 % similarity	452:468	Comparison of the 16S rRNA gene sequences showed that strain CFH S0170T belonged to the genus Paenibacillus and showed closest relationship with Paenibacillus vulneris CCUG 53270T (98.1 % similarity).
29701575	3	32	theme	Paenibacillus	416:428	arg1	53270T					444:449	Paenibacillus vulneris CCUG 53270T	416:449	Paenibacillus vulneris CCUG 53270T (98.1 % similarity)	416:469	Comparison of the 16S rRNA gene sequences showed that strain CFH S0170T belonged to the genus Paenibacillus and showed closest relationship with Paenibacillus vulneris CCUG 53270T (98.1 % similarity).
29701575	12	33	dep	analysis	1301:1308	arg1	the					1244:1246	the	1244:1246	the	1244:1246	On the basis of phenotypic, chemotaxonomic and phylogenetic analysis, strain CFH S0170T is affiliated to the genus Paenibacillus, but could be distinguished from other valid species of this genus.
29701575	12	33	dep	analysis	1301:1308	arg1	basis					1248:1252	basis	1248:1252	basis	1248:1252	On the basis of phenotypic, chemotaxonomic and phylogenetic analysis, strain CFH S0170T is affiliated to the genus Paenibacillus, but could be distinguished from other valid species of this genus.
29701575	10	34	theme	dominant	1130:1137	arg1	acids					1154:1158	The dominant cellular fatty acids	1126:1158	The dominant cellular fatty acids	1126:1158	The dominant cellular fatty acids included anteiso-C15 : 0 and C15 : 0.
29701575	6	35	theme	%	802:802	arg1	NaCl					810:813	0-2.0 % (w/v) NaCl	796:813	0-2.0 % (w/v) NaCl	796:813	Growth of strain CFH S0170T occurred at 10-40 °C, pH 6.0-8.0 and with 0-2.0 % (w/v) NaCl.
29701575	4	36	theme	Phylogenetic	472:483	arg1	analysis					485:492	Phylogenetic analysis	472:492	Phylogenetic analysis	472:492	Phylogenetic analysis demonstrated that the novel candidate formed a coherent branch with P. vulneris CCUG 53270T and Paenibacillus yunnanensis YN2T.
29701575	11	37	theme	DNA	1210:1212	arg1	%					1238:1238	50.9 mol%	1230:1238	50.9 mol%	1230:1238	The genomic DNA G+C content was 50.9 mol%.
29701575	11	37	theme	DNA	1210:1212	arg1	G+C content					1214:1224	The genomic DNA G+C content	1198:1224	The genomic DNA G+C content	1198:1224	The genomic DNA G+C content was 50.9 mol%.
29701575	2	38	theme	CFH	158:160	arg1	S0170T					162:167	CFH S0170T	158:167	CFH S0170T	158:167	A Gram-stain-positive, aerobic, motile with peritrichous flagella, rod-shaped bacterium, designated CFH S0170T, was isolated from a soil sample collected from Catba island in Ha Long Bay, Hai Phong City, Vietnam.
29701575	3	39	theme	CCUG	439:442	arg1	similarity					459:468	98.1 % similarity	452:468	98.1 % similarity	452:468	Comparison of the 16S rRNA gene sequences showed that strain CFH S0170T belonged to the genus Paenibacillus and showed closest relationship with Paenibacillus vulneris CCUG 53270T (98.1 % similarity).
29701575	3	39	theme	CCUG	439:442	arg1	53270T					444:449	Paenibacillus vulneris CCUG 53270T	416:449	Paenibacillus vulneris CCUG 53270T (98.1 % similarity)	416:469	Comparison of the 16S rRNA gene sequences showed that strain CFH S0170T belonged to the genus Paenibacillus and showed closest relationship with Paenibacillus vulneris CCUG 53270T (98.1 % similarity).
29701575	12	40	theme	genus	1431:1435	arg1	species					1415:1421	other valid species	1403:1421	other valid species of this genus	1403:1435	On the basis of phenotypic, chemotaxonomic and phylogenetic analysis, strain CFH S0170T is affiliated to the genus Paenibacillus, but could be distinguished from other valid species of this genus.
29701575	0	41	dep	sp	28:29	arg1	esterisolvens					14:26	Paenibacillus esterisolvens	0:26	Paenibacillus esterisolvens	0:26	Paenibacillus esterisolvens sp.
29701575	4	42	theme	P.	562:563	arg1	53270T					579:584	P. vulneris CCUG 53270T	562:584	P. vulneris CCUG 53270T	562:584	Phylogenetic analysis demonstrated that the novel candidate formed a coherent branch with P. vulneris CCUG 53270T and Paenibacillus yunnanensis YN2T.
29701575	10	43	theme	fatty	1148:1152	arg1	acids					1154:1158	The dominant cellular fatty acids	1126:1158	The dominant cellular fatty acids	1126:1158	The dominant cellular fatty acids included anteiso-C15 : 0 and C15 : 0.
29701575	13	44	dep	sp	1602:1603	arg1	esterisolvens					1588:1600	the name Paenibacillus esterisolvens	1565:1600	the name Paenibacillus esterisolvens	1565:1600	It is concluded that strain CFH S0170T should be considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus esterisolvens sp.
29701575	4	45	theme	CCUG	574:577	arg1	53270T					579:584	P. vulneris CCUG 53270T	562:584	P. vulneris CCUG 53270T	562:584	Phylogenetic analysis demonstrated that the novel candidate formed a coherent branch with P. vulneris CCUG 53270T and Paenibacillus yunnanensis YN2T.
29701575	3	46	theme	%	457:457	arg1	similarity					459:468	98.1 % similarity	452:468	98.1 % similarity	452:468	Comparison of the 16S rRNA gene sequences showed that strain CFH S0170T belonged to the genus Paenibacillus and showed closest relationship with Paenibacillus vulneris CCUG 53270T (98.1 % similarity).
29701575	3	46	theme	%	457:457	arg1	53270T					444:449	Paenibacillus vulneris CCUG 53270T	416:449	Paenibacillus vulneris CCUG 53270T (98.1 % similarity)	416:469	Comparison of the 16S rRNA gene sequences showed that strain CFH S0170T belonged to the genus Paenibacillus and showed closest relationship with Paenibacillus vulneris CCUG 53270T (98.1 % similarity).
29701575	7	47	theme	Strain	816:821	arg1	S0170T					827:832	Strain CFH S0170T	816:832	Strain CFH S0170T	816:832	Strain CFH S0170T contained mannose, glucose and rhamnose as the major whole-cell sugars.
29701575	12	48	theme	valid	1409:1413	arg1	species					1415:1421	other valid species	1403:1421	other valid species of this genus	1403:1435	On the basis of phenotypic, chemotaxonomic and phylogenetic analysis, strain CFH S0170T is affiliated to the genus Paenibacillus, but could be distinguished from other valid species of this genus.
29701575	8	49	theme	cell-wall	910:918	arg1	peptidoglycan					920:932	The cell-wall peptidoglycan	906:932	The cell-wall peptidoglycan	906:932	The cell-wall peptidoglycan contained meso-diaminopimelic acid, glutamic acid, lysine and aspartic acid.
29701575	13	50	theme	genus	1534:1538	arg1	species					1519:1525	a novel species	1511:1525	a novel species	1511:1525	It is concluded that strain CFH S0170T should be considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus esterisolvens sp.
29701575	6	51	theme	CFH	743:745	arg1	S0170T					747:752	strain CFH S0170T	736:752	strain CFH S0170T	736:752	Growth of strain CFH S0170T occurred at 10-40 °C, pH 6.0-8.0 and with 0-2.0 % (w/v) NaCl.
29701575	12	52	theme	CFH	1318:1320	arg1	S0170T					1322:1327	strain CFH S0170T	1311:1327	strain CFH S0170T	1311:1327	On the basis of phenotypic, chemotaxonomic and phylogenetic analysis, strain CFH S0170T is affiliated to the genus Paenibacillus, but could be distinguished from other valid species of this genus.
29701575	3	53	with	relationship	398:409	arg1	similarity					459:468	98.1 % similarity	452:468	98.1 % similarity	452:468	Comparison of the 16S rRNA gene sequences showed that strain CFH S0170T belonged to the genus Paenibacillus and showed closest relationship with Paenibacillus vulneris CCUG 53270T (98.1 % similarity).
29701575	3	53	with	relationship	398:409	arg1	53270T					444:449	Paenibacillus vulneris CCUG 53270T	416:449	Paenibacillus vulneris CCUG 53270T (98.1 % similarity)	416:469	Comparison of the 16S rRNA gene sequences showed that strain CFH S0170T belonged to the genus Paenibacillus and showed closest relationship with Paenibacillus vulneris CCUG 53270T (98.1 % similarity).
29701575	13	54	theme	novel	1513:1517	arg1	species					1519:1525	a novel species	1511:1525	a novel species	1511:1525	It is concluded that strain CFH S0170T should be considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus esterisolvens sp.
29701575	5	55	theme	gene	671:674	arg1	similarity					685:694	87.2 % rpoB gene sequence similarity	659:694	87.2 % rpoB gene sequence similarity with P. vulneris CCUG 53270T	659:723	Furthermore, the novel strain shared 87.2 % rpoB gene sequence similarity with P. vulneris CCUG 53270T.
29701575	2	56	theme	Catba	217:221	arg1	island					223:228	Catba island	217:228	Catba island in Ha Long Bay, Hai Phong City, Vietnam	217:268	A Gram-stain-positive, aerobic, motile with peritrichous flagella, rod-shaped bacterium, designated CFH S0170T, was isolated from a soil sample collected from Catba island in Ha Long Bay, Hai Phong City, Vietnam.
29701575	12	57	theme	phylogenetic	1288:1299	arg1	analysis					1301:1308	phenotypic, chemotaxonomic and phylogenetic analysis	1257:1308	phenotypic, chemotaxonomic and phylogenetic analysis	1257:1308	On the basis of phenotypic, chemotaxonomic and phylogenetic analysis, strain CFH S0170T is affiliated to the genus Paenibacillus, but could be distinguished from other valid species of this genus.
29701575	4	58	theme	yunnanensis	604:614	arg1	YN2T					616:619	Paenibacillus yunnanensis YN2T	590:619	Paenibacillus yunnanensis YN2T	590:619	Phylogenetic analysis demonstrated that the novel candidate formed a coherent branch with P. vulneris CCUG 53270T and Paenibacillus yunnanensis YN2T.
29701575	5	59	theme	novel	639:643	arg1	strain					645:650	the novel strain	635:650	the novel strain	635:650	Furthermore, the novel strain shared 87.2 % rpoB gene sequence similarity with P. vulneris CCUG 53270T.
29701575	5	60	theme	%	664:664	arg1	similarity					685:694	87.2 % rpoB gene sequence similarity	659:694	87.2 % rpoB gene sequence similarity with P. vulneris CCUG 53270T	659:723	Furthermore, the novel strain shared 87.2 % rpoB gene sequence similarity with P. vulneris CCUG 53270T.
29701575	12	61	theme	phenotypic	1257:1266	arg1	analysis					1301:1308	phenotypic, chemotaxonomic and phylogenetic analysis	1257:1308	phenotypic, chemotaxonomic and phylogenetic analysis	1257:1308	On the basis of phenotypic, chemotaxonomic and phylogenetic analysis, strain CFH S0170T is affiliated to the genus Paenibacillus, but could be distinguished from other valid species of this genus.
29701575	15	62	theme	=KCTC	1655:1659	arg1	S0170T					1647:1652	CFH S0170T	1643:1652	CFH S0170T (=KCTC 33624T=BCRC 80802T)	1643:1679	The type strain is CFH S0170T (=KCTC 33624T=BCRC 80802T).
29701575	15	62	theme	=KCTC	1655:1659	arg1	80802T					1673:1678	=KCTC 33624T=BCRC 80802T	1655:1678	=KCTC 33624T=BCRC 80802T	1655:1678	The type strain is CFH S0170T (=KCTC 33624T=BCRC 80802T).
29701575	5	63	theme	CCUG	713:716	arg1	53270T					718:723	P. vulneris CCUG 53270T	701:723	P. vulneris CCUG 53270T	701:723	Furthermore, the novel strain shared 87.2 % rpoB gene sequence similarity with P. vulneris CCUG 53270T.
29701575	4	64	dep	P.	562:563	arg1	vulneris					565:572	vulneris	565:572	vulneris	565:572	Phylogenetic analysis demonstrated that the novel candidate formed a coherent branch with P. vulneris CCUG 53270T and Paenibacillus yunnanensis YN2T.
29701575	13	65	theme	CFH	1466:1468	arg1	S0170T					1470:1475	strain CFH S0170T	1459:1475	strain CFH S0170T	1459:1475	It is concluded that strain CFH S0170T should be considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus esterisolvens sp.
29701575	3	66	theme	16S	289:291	arg1	sequences					303:311	the 16S rRNA gene sequences	285:311	the 16S rRNA gene sequences	285:311	Comparison of the 16S rRNA gene sequences showed that strain CFH S0170T belonged to the genus Paenibacillus and showed closest relationship with Paenibacillus vulneris CCUG 53270T (98.1 % similarity).
29701575	12	67	theme	chemotaxonomic	1269:1282	arg1	analysis					1301:1308	phenotypic, chemotaxonomic and phylogenetic analysis	1257:1308	phenotypic, chemotaxonomic and phylogenetic analysis	1257:1308	On the basis of phenotypic, chemotaxonomic and phylogenetic analysis, strain CFH S0170T is affiliated to the genus Paenibacillus, but could be distinguished from other valid species of this genus.
29701575	8	68	contain	contained	934:942	arg2	acid					964:967	meso-diaminopimelic acid	944:967	meso-diaminopimelic acid	944:967	The cell-wall peptidoglycan contained meso-diaminopimelic acid, glutamic acid, lysine and aspartic acid.
29701575	8	68	contain	contained	934:942	arg2	acid					1005:1008	aspartic acid	996:1008	aspartic acid	996:1008	The cell-wall peptidoglycan contained meso-diaminopimelic acid, glutamic acid, lysine and aspartic acid.
29701575	8	68	contain	contained	934:942	arg2	lysine					985:990	lysine	985:990	lysine	985:990	The cell-wall peptidoglycan contained meso-diaminopimelic acid, glutamic acid, lysine and aspartic acid.
29701575	8	68	contain	contained	934:942	arg1	peptidoglycan					920:932	The cell-wall peptidoglycan	906:932	The cell-wall peptidoglycan	906:932	The cell-wall peptidoglycan contained meso-diaminopimelic acid, glutamic acid, lysine and aspartic acid.
29701575	8	68	contain	contained	934:942	arg2	acid					979:982	glutamic acid	970:982	glutamic acid	970:982	The cell-wall peptidoglycan contained meso-diaminopimelic acid, glutamic acid, lysine and aspartic acid.
29701575	8	69	theme	glutamic	970:977	arg1	acid					979:982	glutamic acid	970:982	glutamic acid	970:982	The cell-wall peptidoglycan contained meso-diaminopimelic acid, glutamic acid, lysine and aspartic acid.
29701575	7	70	theme	whole-cell	887:896	arg1	sugars					898:903	the major whole-cell sugars	877:903	the major whole-cell sugars	877:903	Strain CFH S0170T contained mannose, glucose and rhamnose as the major whole-cell sugars.
29701575	7	70	theme	whole-cell	887:896	arg1	rhamnose					865:872	rhamnose	865:872	rhamnose	865:872	Strain CFH S0170T contained mannose, glucose and rhamnose as the major whole-cell sugars.
29701575	7	70	theme	whole-cell	887:896	arg1	glucose					853:859	glucose	853:859	glucose	853:859	Strain CFH S0170T contained mannose, glucose and rhamnose as the major whole-cell sugars.
29701575	7	70	theme	whole-cell	887:896	arg1	mannose					844:850	mannose	844:850	mannose	844:850	Strain CFH S0170T contained mannose, glucose and rhamnose as the major whole-cell sugars.
29701575	11	71	theme	50.9 mol	1230:1237	arg1	%					1238:1238	50.9 mol%	1230:1238	50.9 mol%	1230:1238	The genomic DNA G+C content was 50.9 mol%.
29701575	11	71	theme	50.9 mol	1230:1237	arg1	G+C content					1214:1224	The genomic DNA G+C content	1198:1224	The genomic DNA G+C content	1198:1224	The genomic DNA G+C content was 50.9 mol%.
29701575	5	72	theme	P.	701:702	arg1	53270T					718:723	P. vulneris CCUG 53270T	701:723	P. vulneris CCUG 53270T	701:723	Furthermore, the novel strain shared 87.2 % rpoB gene sequence similarity with P. vulneris CCUG 53270T.
29701575	3	73	theme	gene	298:301	arg1	sequences					303:311	the 16S rRNA gene sequences	285:311	the 16S rRNA gene sequences	285:311	Comparison of the 16S rRNA gene sequences showed that strain CFH S0170T belonged to the genus Paenibacillus and showed closest relationship with Paenibacillus vulneris CCUG 53270T (98.1 % similarity).
29701575	2	74	dep	Gram-stain-positive	60:78	arg1	aerobic					81:87	aerobic	81:87	aerobic	81:87	A Gram-stain-positive, aerobic, motile with peritrichous flagella, rod-shaped bacterium, designated CFH S0170T, was isolated from a soil sample collected from Catba island in Ha Long Bay, Hai Phong City, Vietnam.
29701575	2	74	dep	Gram-stain-positive	60:78	arg1	rod-shaped					125:134	rod-shaped	125:134	rod-shaped	125:134	A Gram-stain-positive, aerobic, motile with peritrichous flagella, rod-shaped bacterium, designated CFH S0170T, was isolated from a soil sample collected from Catba island in Ha Long Bay, Hai Phong City, Vietnam.
29701575	2	74	dep	Gram-stain-positive	60:78	arg1	motile					90:95	motile	90:95	motile	90:95	A Gram-stain-positive, aerobic, motile with peritrichous flagella, rod-shaped bacterium, designated CFH S0170T, was isolated from a soil sample collected from Catba island in Ha Long Bay, Hai Phong City, Vietnam.
29701575	9	75	theme	polar	1015:1019	arg1	profile					1027:1033	The polar lipid profile	1011:1033	The polar lipid profile	1011:1033	The polar lipid profile contained diphosphatidylglycerol, phosphatidylethanolamine, glycolipids and phospholipids.
29701575	3	76	theme	closest	390:396	arg1	relationship					398:409	closest relationship	390:409	closest relationship with Paenibacillus vulneris CCUG 53270T (98.1 % similarity)	390:469	Comparison of the 16S rRNA gene sequences showed that strain CFH S0170T belonged to the genus Paenibacillus and showed closest relationship with Paenibacillus vulneris CCUG 53270T (98.1 % similarity).
29701575	2	77	attach	isolated	174:181	arg2	bacterium					136:144	A Gram-stain-positive, aerobic, motile with peritrichous flagella, rod-shaped bacterium	58:144	A Gram-stain-positive, aerobic, motile with peritrichous flagella, rod-shaped bacterium	58:144	A Gram-stain-positive, aerobic, motile with peritrichous flagella, rod-shaped bacterium, designated CFH S0170T, was isolated from a soil sample collected from Catba island in Ha Long Bay, Hai Phong City, Vietnam.
29701575	2	77	attach	isolated	174:181	arg1	sample					195:200	a soil sample	188:200	a soil sample collected from Catba island in Ha Long Bay, Hai Phong City, Vietnam	188:268	A Gram-stain-positive, aerobic, motile with peritrichous flagella, rod-shaped bacterium, designated CFH S0170T, was isolated from a soil sample collected from Catba island in Ha Long Bay, Hai Phong City, Vietnam.
29701575	4	78	theme	novel	516:520	arg1	candidate					522:530	the novel candidate	512:530	the novel candidate	512:530	Phylogenetic analysis demonstrated that the novel candidate formed a coherent branch with P. vulneris CCUG 53270T and Paenibacillus yunnanensis YN2T.
29701575	7	79	contain	contained	834:842	arg2	mannose					844:850	mannose	844:850	mannose	844:850	Strain CFH S0170T contained mannose, glucose and rhamnose as the major whole-cell sugars.
29701575	7	79	contain	contained	834:842	arg1	S0170T					827:832	Strain CFH S0170T	816:832	Strain CFH S0170T	816:832	Strain CFH S0170T contained mannose, glucose and rhamnose as the major whole-cell sugars.
29701575	7	79	contain	contained	834:842	arg2	rhamnose					865:872	rhamnose	865:872	rhamnose	865:872	Strain CFH S0170T contained mannose, glucose and rhamnose as the major whole-cell sugars.
29701575	7	79	contain	contained	834:842	arg2	glucose					853:859	glucose	853:859	glucose	853:859	Strain CFH S0170T contained mannose, glucose and rhamnose as the major whole-cell sugars.
29701575	7	79	contain	contained	834:842	arg2	sugars					898:903	the major whole-cell sugars	877:903	the major whole-cell sugars	877:903	Strain CFH S0170T contained mannose, glucose and rhamnose as the major whole-cell sugars.
29701575	10	80	dep	 0	1194:1195	arg1	C15 					1189:1192	C15 	1189:1192	C15 	1189:1192	The dominant cellular fatty acids included anteiso-C15 : 0 and C15 : 0.
29701575	10	80	dep	 0	1194:1195	arg1	 0					1182:1183	 0	1182:1183	 0	1182:1183	The dominant cellular fatty acids included anteiso-C15 : 0 and C15 : 0.
29701575	2	81	from	island	223:228	arg1	Hai					246:248	Hai	246:248	Hai	246:248	A Gram-stain-positive, aerobic, motile with peritrichous flagella, rod-shaped bacterium, designated CFH S0170T, was isolated from a soil sample collected from Catba island in Ha Long Bay, Hai Phong City, Vietnam.
29701575	2	81	from	island	223:228	arg1	Vietnam					262:268	Vietnam	262:268	Vietnam	262:268	A Gram-stain-positive, aerobic, motile with peritrichous flagella, rod-shaped bacterium, designated CFH S0170T, was isolated from a soil sample collected from Catba island in Ha Long Bay, Hai Phong City, Vietnam.
29701575	2	81	from	island	223:228	arg1	City					256:259	City	256:259	City	256:259	A Gram-stain-positive, aerobic, motile with peritrichous flagella, rod-shaped bacterium, designated CFH S0170T, was isolated from a soil sample collected from Catba island in Ha Long Bay, Hai Phong City, Vietnam.
29701575	3	82	theme	strain	325:330	arg1	S0170T					336:341	strain CFH S0170T	325:341	strain CFH S0170T	325:341	Comparison of the 16S rRNA gene sequences showed that strain CFH S0170T belonged to the genus Paenibacillus and showed closest relationship with Paenibacillus vulneris CCUG 53270T (98.1 % similarity).
29701575	13	83	theme	Paenibacillus	1574:1586	arg1	esterisolvens					1588:1600	the name Paenibacillus esterisolvens	1565:1600	the name Paenibacillus esterisolvens	1565:1600	It is concluded that strain CFH S0170T should be considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus esterisolvens sp.
29701575	3	84	theme	vulneris	430:437	arg1	similarity					459:468	98.1 % similarity	452:468	98.1 % similarity	452:468	Comparison of the 16S rRNA gene sequences showed that strain CFH S0170T belonged to the genus Paenibacillus and showed closest relationship with Paenibacillus vulneris CCUG 53270T (98.1 % similarity).
29701575	3	84	theme	vulneris	430:437	arg1	53270T					444:449	Paenibacillus vulneris CCUG 53270T	416:449	Paenibacillus vulneris CCUG 53270T (98.1 % similarity)	416:469	Comparison of the 16S rRNA gene sequences showed that strain CFH S0170T belonged to the genus Paenibacillus and showed closest relationship with Paenibacillus vulneris CCUG 53270T (98.1 % similarity).
29701575	6	85	dep	%	802:802	arg1	w/v					805:807	w/v	805:807	w/v	805:807	Growth of strain CFH S0170T occurred at 10-40 °C, pH 6.0-8.0 and with 0-2.0 % (w/v) NaCl.
29701575	6	86	theme	0-2.0 	796:801	arg1	%					802:802	%	802:802	%	802:802	Growth of strain CFH S0170T occurred at 10-40 °C, pH 6.0-8.0 and with 0-2.0 % (w/v) NaCl.
29701575	2	87	theme	soil	190:193	arg1	sample					195:200	a soil sample	188:200	a soil sample collected from Catba island in Ha Long Bay, Hai Phong City, Vietnam	188:268	A Gram-stain-positive, aerobic, motile with peritrichous flagella, rod-shaped bacterium, designated CFH S0170T, was isolated from a soil sample collected from Catba island in Ha Long Bay, Hai Phong City, Vietnam.
29701575	3	88	theme	98.1 	452:456	arg1	%					457:457	%	457:457	%	457:457	Comparison of the 16S rRNA gene sequences showed that strain CFH S0170T belonged to the genus Paenibacillus and showed closest relationship with Paenibacillus vulneris CCUG 53270T (98.1 % similarity).
29701575	11	89	theme	genomic	1202:1208	arg1	%					1238:1238	50.9 mol%	1230:1238	50.9 mol%	1230:1238	The genomic DNA G+C content was 50.9 mol%.
29701575	11	89	theme	genomic	1202:1208	arg1	G+C content					1214:1224	The genomic DNA G+C content	1198:1224	The genomic DNA G+C content	1198:1224	The genomic DNA G+C content was 50.9 mol%.
29701575	7	90	theme	CFH	823:825	arg1	S0170T					827:832	Strain CFH S0170T	816:832	Strain CFH S0170T	816:832	Strain CFH S0170T contained mannose, glucose and rhamnose as the major whole-cell sugars.
29701575	10	91	theme	cellular	1139:1146	arg1	acids					1154:1158	The dominant cellular fatty acids	1126:1158	The dominant cellular fatty acids	1126:1158	The dominant cellular fatty acids included anteiso-C15 : 0 and C15 : 0.
30028286	11	0	theme	genus	1362:1366	arg1	Nocardioides					1368:1379	the genus Nocardioides	1358:1379	the genus Nocardioides	1358:1379	On the basis of the phenotypic, chemotaxonomic and molecular features, IB-3T represents a novel species of the genus Nocardioides, for which the name Nocardioidescurrus sp.
30028286	2	1	theme	bacterial	121:129	arg1	strain					131:136	A strictly aerobic Gram-stain-positive bacterial strain	82:136	A strictly aerobic Gram-stain-positive bacterial strain	82:136	A strictly aerobic Gram-stain-positive bacterial strain, designated IB-3T, was isolated from a car air-conditioning system in the Republic of Korea.
30028286	7	2	theme	peptidoglycan	848:860	arg1	type					862:865	The major cell wall peptidoglycan type	828:865	The major cell wall peptidoglycan type	828:865	The major cell wall peptidoglycan type was ll-2,6-diaminopimelic acid.
30028286	7	2	theme	peptidoglycan	848:860	arg1	acid					893:896	ll-2,6-diaminopimelic acid	871:896	ll-2,6-diaminopimelic acid	871:896	The major cell wall peptidoglycan type was ll-2,6-diaminopimelic acid.
30028286	5	3	theme	cellular	674:681	arg1	 0					539:540	 0	539:540	 0	539:540	Menaquinone-8 (H4) was detected as the predominant respiratory quinone and iso-C16 : 0, 10-methyl-C17 : 0, iso-C17 : 0, C18 : 1ω9c, C17 : 1ω8c, C18 : 0, 10-methyl-C18 : 0 (TBSA) and C17 : 0 were identified as the major cellular fatty acids.
30028286	5	3	theme	cellular	674:681	arg1	acids					689:693	the major cellular fatty acids	664:693	the major cellular fatty acids	664:693	Menaquinone-8 (H4) was detected as the predominant respiratory quinone and iso-C16 : 0, 10-methyl-C17 : 0, iso-C17 : 0, C18 : 1ω9c, C17 : 1ω8c, C18 : 0, 10-methyl-C18 : 0 (TBSA) and C17 : 0 were identified as the major cellular fatty acids.
30028286	10	4	theme	phylogenetic	1076:1087	arg1	analyses					1089:1096	phylogenetic analyses	1076:1096	phylogenetic analyses based on 16S rRNA gene sequences	1076:1129	The results of phylogenetic analyses based on 16S rRNA gene sequences indicated that IB-3T formed a distinct phylogenetic lineage within the genus Nocardioidesof the family Nocardioidaceae.
30028286	2	5	theme	Gram-stain-positive	101:119	arg1	strain					131:136	A strictly aerobic Gram-stain-positive bacterial strain	82:136	A strictly aerobic Gram-stain-positive bacterial strain	82:136	A strictly aerobic Gram-stain-positive bacterial strain, designated IB-3T, was isolated from a car air-conditioning system in the Republic of Korea.
30028286	10	6	theme	family	1227:1232	arg1	Nocardioidaceae					1234:1248	the family Nocardioidaceae	1223:1248	the genus Nocardioidesof the family Nocardioidaceae	1198:1248	The results of phylogenetic analyses based on 16S rRNA gene sequences indicated that IB-3T formed a distinct phylogenetic lineage within the genus Nocardioidesof the family Nocardioidaceae.
30028286	5	7	theme	fatty	683:687	arg1	 0					539:540	 0	539:540	 0	539:540	Menaquinone-8 (H4) was detected as the predominant respiratory quinone and iso-C16 : 0, 10-methyl-C17 : 0, iso-C17 : 0, C18 : 1ω9c, C17 : 1ω8c, C18 : 0, 10-methyl-C18 : 0 (TBSA) and C17 : 0 were identified as the major cellular fatty acids.
30028286	5	7	theme	fatty	683:687	arg1	acids					689:693	the major cellular fatty acids	664:693	the major cellular fatty acids	664:693	Menaquinone-8 (H4) was detected as the predominant respiratory quinone and iso-C16 : 0, 10-methyl-C17 : 0, iso-C17 : 0, C18 : 1ω9c, C17 : 1ω8c, C18 : 0, 10-methyl-C18 : 0 (TBSA) and C17 : 0 were identified as the major cellular fatty acids.
30028286	6	8	theme	polar	814:818	arg1	phosphatidylcholine					742:760	phosphatidylcholine	742:760	phosphatidylcholine	742:760	Phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine and phosphatidylinositol were detected as the major polar lipids.
30028286	6	8	theme	polar	814:818	arg1	diphosphatidylglycerol					718:739	diphosphatidylglycerol	718:739	diphosphatidylglycerol	718:739	Phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine and phosphatidylinositol were detected as the major polar lipids.
30028286	6	8	theme	polar	814:818	arg1	lipids					820:825	the major polar lipids	804:825	the major polar lipids	804:825	Phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine and phosphatidylinositol were detected as the major polar lipids.
30028286	6	8	theme	polar	814:818	arg1	Phosphatidylglycerol					696:715	Phosphatidylglycerol	696:715	Phosphatidylglycerol	696:715	Phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine and phosphatidylinositol were detected as the major polar lipids.
30028286	6	8	theme	polar	814:818	arg1	phosphatidylinositol					766:785	phosphatidylinositol	766:785	phosphatidylinositol	766:785	Phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine and phosphatidylinositol were detected as the major polar lipids.
30028286	2	9	theme	aerobic	93:99	arg1	strain					131:136	A strictly aerobic Gram-stain-positive bacterial strain	82:136	A strictly aerobic Gram-stain-positive bacterial strain	82:136	A strictly aerobic Gram-stain-positive bacterial strain, designated IB-3T, was isolated from a car air-conditioning system in the Republic of Korea.
30028286	10	10	theme	genus	1202:1206	arg1	Nocardioidesof					1208:1221	the genus Nocardioidesof the family Nocardioidaceae	1198:1248	the genus Nocardioidesof the family Nocardioidaceae	1198:1248	The results of phylogenetic analyses based on 16S rRNA gene sequences indicated that IB-3T formed a distinct phylogenetic lineage within the genus Nocardioidesof the family Nocardioidaceae.
30028286	9	11	theme	Nocardioides	983:994	arg1	terrigenaDS-17T					996:1010	Nocardioides terrigenaDS-17T	983:1010	Nocardioides terrigenaDS-17T with a 98.0 % 16S rRNA gene sequence similarity	983:1058	IB-3T was most closely related to Nocardioides terrigenaDS-17T with a 98.0 % 16S rRNA gene sequence similarity.
30028286	6	12	theme	major	808:812	arg1	phosphatidylcholine					742:760	phosphatidylcholine	742:760	phosphatidylcholine	742:760	Phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine and phosphatidylinositol were detected as the major polar lipids.
30028286	6	12	theme	major	808:812	arg1	diphosphatidylglycerol					718:739	diphosphatidylglycerol	718:739	diphosphatidylglycerol	718:739	Phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine and phosphatidylinositol were detected as the major polar lipids.
30028286	6	12	theme	major	808:812	arg1	lipids					820:825	the major polar lipids	804:825	the major polar lipids	804:825	Phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine and phosphatidylinositol were detected as the major polar lipids.
30028286	6	12	theme	major	808:812	arg1	Phosphatidylglycerol					696:715	Phosphatidylglycerol	696:715	Phosphatidylglycerol	696:715	Phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine and phosphatidylinositol were detected as the major polar lipids.
30028286	6	12	theme	major	808:812	arg1	phosphatidylinositol					766:785	phosphatidylinositol	766:785	phosphatidylinositol	766:785	Phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine and phosphatidylinositol were detected as the major polar lipids.
30028286	1	13	theme	mobile	46:51	arg1	system					74:79	a mobile car air-conditioning system	44:79	a mobile car air-conditioning system	44:79	nov., isolated from a mobile car air-conditioning system.
30028286	4	14	dep	optimum	440:446	arg1	%					451:451	0 %	449:451	0 %	449:451	Growth of IB-3T was observed at 20-40 °C (optimum, 25 °C), at pH 6.5-9.0 (optimum, pH 7.5) and in the presence of 0-1 % (w/v) NaCl (optimum, 0 %).
30028286	10	15	theme	rRNA	1111:1114	arg1	sequences					1121:1129	16S rRNA gene sequences	1107:1129	16S rRNA gene sequences	1107:1129	The results of phylogenetic analyses based on 16S rRNA gene sequences indicated that IB-3T formed a distinct phylogenetic lineage within the genus Nocardioidesof the family Nocardioidaceae.
30028286	5	16	theme	respiratory	506:516	arg1	quinone					518:524	the predominant respiratory quinone	490:524	the predominant respiratory quinone	490:524	Menaquinone-8 (H4) was detected as the predominant respiratory quinone and iso-C16 : 0, 10-methyl-C17 : 0, iso-C17 : 0, C18 : 1ω9c, C17 : 1ω8c, C18 : 0, 10-methyl-C18 : 0 (TBSA) and C17 : 0 were identified as the major cellular fatty acids.
30028286	5	16	theme	respiratory	506:516	arg1	Menaquinone-8					455:467	Menaquinone-8	455:467	Menaquinone-8 (H4)	455:472	Menaquinone-8 (H4) was detected as the predominant respiratory quinone and iso-C16 : 0, 10-methyl-C17 : 0, iso-C17 : 0, C18 : 1ω9c, C17 : 1ω8c, C18 : 0, 10-methyl-C18 : 0 (TBSA) and C17 : 0 were identified as the major cellular fatty acids.
30028286	5	17	theme	predominant	494:504	arg1	quinone					518:524	the predominant respiratory quinone	490:524	the predominant respiratory quinone	490:524	Menaquinone-8 (H4) was detected as the predominant respiratory quinone and iso-C16 : 0, 10-methyl-C17 : 0, iso-C17 : 0, C18 : 1ω9c, C17 : 1ω8c, C18 : 0, 10-methyl-C18 : 0 (TBSA) and C17 : 0 were identified as the major cellular fatty acids.
30028286	5	17	theme	predominant	494:504	arg1	Menaquinone-8					455:467	Menaquinone-8	455:467	Menaquinone-8 (H4)	455:472	Menaquinone-8 (H4) was detected as the predominant respiratory quinone and iso-C16 : 0, 10-methyl-C17 : 0, iso-C17 : 0, C18 : 1ω9c, C17 : 1ω8c, C18 : 0, 10-methyl-C18 : 0 (TBSA) and C17 : 0 were identified as the major cellular fatty acids.
30028286	1	18	theme	car	53:55	arg1	system					74:79	a mobile car air-conditioning system	44:79	a mobile car air-conditioning system	44:79	nov., isolated from a mobile car air-conditioning system.
30028286	10	19	theme	16S	1107:1109	arg1	sequences					1121:1129	16S rRNA gene sequences	1107:1129	16S rRNA gene sequences	1107:1129	The results of phylogenetic analyses based on 16S rRNA gene sequences indicated that IB-3T formed a distinct phylogenetic lineage within the genus Nocardioidesof the family Nocardioidaceae.
30028286	2	20	from	system	198:203	arg1	Republic					212:219	the Republic	208:219	the Republic of Korea	208:228	A strictly aerobic Gram-stain-positive bacterial strain, designated IB-3T, was isolated from a car air-conditioning system in the Republic of Korea.
30028286	4	21	theme	%	426:426	arg1	optimum					440:446	optimum	440:446	optimum	440:446	Growth of IB-3T was observed at 20-40 °C (optimum, 25 °C), at pH 6.5-9.0 (optimum, pH 7.5) and in the presence of 0-1 % (w/v) NaCl (optimum, 0 %).
30028286	4	21	theme	%	426:426	arg1	NaCl					434:437	0-1 % (w/v) NaCl	422:437	0-1 % (w/v) NaCl (optimum, 0 %)	422:452	Growth of IB-3T was observed at 20-40 °C (optimum, 25 °C), at pH 6.5-9.0 (optimum, pH 7.5) and in the presence of 0-1 % (w/v) NaCl (optimum, 0 %).
30028286	3	22	theme	non-motile	242:251	arg1	rods					253:256	non-motile rods	242:256	non-motile rods showing catalase- and oxidase-positive reactions	242:305	Cells were non-motile rods showing catalase- and oxidase-positive reactions.
30028286	9	23	theme	%	1024:1024	arg1	similarity					1049:1058	a 98.0 % 16S rRNA gene sequence similarity	1017:1058	a 98.0 % 16S rRNA gene sequence similarity	1017:1058	IB-3T was most closely related to Nocardioides terrigenaDS-17T with a 98.0 % 16S rRNA gene sequence similarity.
30028286	1	24	theme	air-conditioning	57:72	arg1	system					74:79	a mobile car air-conditioning system	44:79	a mobile car air-conditioning system	44:79	nov., isolated from a mobile car air-conditioning system.
30028286	8	25	theme	genomic	922:928	arg1	DNA					930:932	the genomic DNA	918:932	the genomic DNA	918:932	The G+C content of the genomic DNA was 71.5 mol%.
30028286	7	26	theme	wall	843:846	arg1	type					862:865	The major cell wall peptidoglycan type	828:865	The major cell wall peptidoglycan type	828:865	The major cell wall peptidoglycan type was ll-2,6-diaminopimelic acid.
30028286	7	26	theme	wall	843:846	arg1	acid					893:896	ll-2,6-diaminopimelic acid	871:896	ll-2,6-diaminopimelic acid	871:896	The major cell wall peptidoglycan type was ll-2,6-diaminopimelic acid.
30028286	0	27	theme	currus	13:18	arg1	sp					20:21	Nocardioides currus sp	0:21	Nocardioides currus sp.	0:22	Nocardioides currus sp.
30028286	4	28	theme	0-1 	422:425	arg1	optimum					440:446	optimum	440:446	optimum	440:446	Growth of IB-3T was observed at 20-40 °C (optimum, 25 °C), at pH 6.5-9.0 (optimum, pH 7.5) and in the presence of 0-1 % (w/v) NaCl (optimum, 0 %).
30028286	4	28	theme	0-1 	422:425	arg1	NaCl					434:437	0-1 % (w/v) NaCl	422:437	0-1 % (w/v) NaCl (optimum, 0 %)	422:452	Growth of IB-3T was observed at 20-40 °C (optimum, 25 °C), at pH 6.5-9.0 (optimum, pH 7.5) and in the presence of 0-1 % (w/v) NaCl (optimum, 0 %).
30028286	9	29	theme	16S	1026:1028	arg1	similarity					1049:1058	a 98.0 % 16S rRNA gene sequence similarity	1017:1058	a 98.0 % 16S rRNA gene sequence similarity	1017:1058	IB-3T was most closely related to Nocardioides terrigenaDS-17T with a 98.0 % 16S rRNA gene sequence similarity.
30028286	8	30	theme	DNA	930:932	arg1	G+C content					903:913	The G+C content	899:913	The G+C content of the genomic DNA	899:932	The G+C content of the genomic DNA was 71.5 mol%.
30028286	8	30	theme	DNA	930:932	arg1	%					946:946	71.5 mol%	938:946	71.5 mol%	938:946	The G+C content of the genomic DNA was 71.5 mol%.
30028286	0	31	theme	Nocardioides	0:11	arg1	sp					20:21	Nocardioides currus sp	0:21	Nocardioides currus sp.	0:22	Nocardioides currus sp.
30028286	2	32	theme	car	177:179	arg1	system					198:203	a car air-conditioning system	175:203	a car air-conditioning system in the Republic of Korea	175:228	A strictly aerobic Gram-stain-positive bacterial strain, designated IB-3T, was isolated from a car air-conditioning system in the Republic of Korea.
30028286	9	33	theme	rRNA	1030:1033	arg1	similarity					1049:1058	a 98.0 % 16S rRNA gene sequence similarity	1017:1058	a 98.0 % 16S rRNA gene sequence similarity	1017:1058	IB-3T was most closely related to Nocardioides terrigenaDS-17T with a 98.0 % 16S rRNA gene sequence similarity.
30028286	11	34	theme	name	1396:1399	arg1	sp					1420:1421	the name Nocardioidescurrus sp	1392:1421	the name Nocardioidescurrus sp	1392:1421	On the basis of the phenotypic, chemotaxonomic and molecular features, IB-3T represents a novel species of the genus Nocardioides, for which the name Nocardioidescurrus sp.
30028286	4	35	dep	optimum	350:356	arg1	25 °C					359:363	25 °C	359:363	25 °C	359:363	Growth of IB-3T was observed at 20-40 °C (optimum, 25 °C), at pH 6.5-9.0 (optimum, pH 7.5) and in the presence of 0-1 % (w/v) NaCl (optimum, 0 %).
30028286	10	36	theme	phylogenetic	1170:1181	arg1	lineage					1183:1189	a distinct phylogenetic lineage	1159:1189	a distinct phylogenetic lineage	1159:1189	The results of phylogenetic analyses based on 16S rRNA gene sequences indicated that IB-3T formed a distinct phylogenetic lineage within the genus Nocardioidesof the family Nocardioidaceae.
30028286	10	37	theme	distinct	1161:1168	arg1	lineage					1183:1189	a distinct phylogenetic lineage	1159:1189	a distinct phylogenetic lineage	1159:1189	The results of phylogenetic analyses based on 16S rRNA gene sequences indicated that IB-3T formed a distinct phylogenetic lineage within the genus Nocardioidesof the family Nocardioidaceae.
30028286	3	38	theme	catalase-	266:274	arg1	reactions					297:305	catalase- and oxidase-positive reactions	266:305	catalase- and oxidase-positive reactions	266:305	Cells were non-motile rods showing catalase- and oxidase-positive reactions.
30028286	9	39	theme	gene	1035:1038	arg1	similarity					1049:1058	a 98.0 % 16S rRNA gene sequence similarity	1017:1058	a 98.0 % 16S rRNA gene sequence similarity	1017:1058	IB-3T was most closely related to Nocardioides terrigenaDS-17T with a 98.0 % 16S rRNA gene sequence similarity.
30028286	11	40	theme	Nocardioides	1368:1379	arg1	species					1347:1353	a novel species	1339:1353	a novel species	1339:1353	On the basis of the phenotypic, chemotaxonomic and molecular features, IB-3T represents a novel species of the genus Nocardioides, for which the name Nocardioidescurrus sp.
30028286	5	41	dep	 0	539:540	arg1	C17 					637:640	C17 	637:640	C17 	637:640	Menaquinone-8 (H4) was detected as the predominant respiratory quinone and iso-C16 : 0, 10-methyl-C17 : 0, iso-C17 : 0, C18 : 1ω9c, C17 : 1ω8c, C18 : 0, 10-methyl-C18 : 0 (TBSA) and C17 : 0 were identified as the major cellular fatty acids.
30028286	5	41	dep	 0	539:540	arg1	 0					571:572	 0	571:572	 0	571:572	Menaquinone-8 (H4) was detected as the predominant respiratory quinone and iso-C16 : 0, 10-methyl-C17 : 0, iso-C17 : 0, C18 : 1ω9c, C17 : 1ω8c, C18 : 0, 10-methyl-C18 : 0 (TBSA) and C17 : 0 were identified as the major cellular fatty acids.
30028286	5	41	dep	 0	539:540	arg1	 0					558:559	 0	558:559	 0	558:559	Menaquinone-8 (H4) was detected as the predominant respiratory quinone and iso-C16 : 0, 10-methyl-C17 : 0, iso-C17 : 0, C18 : 1ω9c, C17 : 1ω8c, C18 : 0, 10-methyl-C18 : 0 (TBSA) and C17 : 0 were identified as the major cellular fatty acids.
30028286	5	41	dep	 0	539:540	arg1	 0					642:643	 0	642:643	 0	642:643	Menaquinone-8 (H4) was detected as the predominant respiratory quinone and iso-C16 : 0, 10-methyl-C17 : 0, iso-C17 : 0, C18 : 1ω9c, C17 : 1ω8c, C18 : 0, 10-methyl-C18 : 0 (TBSA) and C17 : 0 were identified as the major cellular fatty acids.
30028286	5	41	dep	 0	539:540	arg1	C18 					575:578	C18 	575:578	C18 	575:578	Menaquinone-8 (H4) was detected as the predominant respiratory quinone and iso-C16 : 0, 10-methyl-C17 : 0, iso-C17 : 0, C18 : 1ω9c, C17 : 1ω8c, C18 : 0, 10-methyl-C18 : 0 (TBSA) and C17 : 0 were identified as the major cellular fatty acids.
30028286	13	42	theme	=KACC	1468:1472	arg1	IB-3T					1461:1465	IB-3T	1461:1465	IB-3T (=KACC 19522T=JCM 32672T)	1461:1491	The type strain is IB-3T (=KACC 19522T=JCM 32672T).
30028286	13	42	theme	=KACC	1468:1472	arg1	32672T					1485:1490	=KACC 19522T=JCM 32672T	1468:1490	=KACC 19522T=JCM 32672T	1468:1490	The type strain is IB-3T (=KACC 19522T=JCM 32672T).
30028286	9	43	theme	sequence	1040:1047	arg1	similarity					1049:1058	a 98.0 % 16S rRNA gene sequence similarity	1017:1058	a 98.0 % 16S rRNA gene sequence similarity	1017:1058	IB-3T was most closely related to Nocardioides terrigenaDS-17T with a 98.0 % 16S rRNA gene sequence similarity.
30028286	11	44	theme	chemotaxonomic	1283:1296	arg1	features					1312:1319	the phenotypic, chemotaxonomic and molecular features	1267:1319	the phenotypic, chemotaxonomic and molecular features	1267:1319	On the basis of the phenotypic, chemotaxonomic and molecular features, IB-3T represents a novel species of the genus Nocardioides, for which the name Nocardioidescurrus sp.
30028286	11	45	theme	phenotypic	1271:1280	arg1	features					1312:1319	the phenotypic, chemotaxonomic and molecular features	1267:1319	the phenotypic, chemotaxonomic and molecular features	1267:1319	On the basis of the phenotypic, chemotaxonomic and molecular features, IB-3T represents a novel species of the genus Nocardioides, for which the name Nocardioidescurrus sp.
30028286	7	46	theme	ll-2,6-diaminopimelic	871:891	arg1	type					862:865	The major cell wall peptidoglycan type	828:865	The major cell wall peptidoglycan type	828:865	The major cell wall peptidoglycan type was ll-2,6-diaminopimelic acid.
30028286	7	46	theme	ll-2,6-diaminopimelic	871:891	arg1	acid					893:896	ll-2,6-diaminopimelic acid	871:896	ll-2,6-diaminopimelic acid	871:896	The major cell wall peptidoglycan type was ll-2,6-diaminopimelic acid.
30028286	2	47	theme	Korea	224:228	arg1	Republic					212:219	the Republic	208:219	the Republic of Korea	208:228	A strictly aerobic Gram-stain-positive bacterial strain, designated IB-3T, was isolated from a car air-conditioning system in the Republic of Korea.
30028286	10	48	theme	gene	1116:1119	arg1	sequences					1121:1129	16S rRNA gene sequences	1107:1129	16S rRNA gene sequences	1107:1129	The results of phylogenetic analyses based on 16S rRNA gene sequences indicated that IB-3T formed a distinct phylogenetic lineage within the genus Nocardioidesof the family Nocardioidaceae.
30028286	13	49	theme	19522T=JCM	1474:1483	arg1	IB-3T					1461:1465	IB-3T	1461:1465	IB-3T (=KACC 19522T=JCM 32672T)	1461:1491	The type strain is IB-3T (=KACC 19522T=JCM 32672T).
30028286	13	49	theme	19522T=JCM	1474:1483	arg1	32672T					1485:1490	=KACC 19522T=JCM 32672T	1468:1490	=KACC 19522T=JCM 32672T	1468:1490	The type strain is IB-3T (=KACC 19522T=JCM 32672T).
30028286	11	50	dep	features	1312:1319	arg1	basis					1258:1262	basis	1258:1262	basis	1258:1262	On the basis of the phenotypic, chemotaxonomic and molecular features, IB-3T represents a novel species of the genus Nocardioides, for which the name Nocardioidescurrus sp.
30028286	11	50	dep	features	1312:1319	arg1	the					1254:1256	the	1254:1256	the	1254:1256	On the basis of the phenotypic, chemotaxonomic and molecular features, IB-3T represents a novel species of the genus Nocardioides, for which the name Nocardioidescurrus sp.
30028286	4	51	theme	NaCl	434:437	arg1	presence					410:417	the presence	406:417	the presence of 0-1 % (w/v) NaCl (optimum, 0 %)	406:452	Growth of IB-3T was observed at 20-40 °C (optimum, 25 °C), at pH 6.5-9.0 (optimum, pH 7.5) and in the presence of 0-1 % (w/v) NaCl (optimum, 0 %).
30028286	4	52	located	observed	328:335	arg2	Growth					308:313	Growth	308:313	Growth of IB-3T	308:322	Growth of IB-3T was observed at 20-40 °C (optimum, 25 °C), at pH 6.5-9.0 (optimum, pH 7.5) and in the presence of 0-1 % (w/v) NaCl (optimum, 0 %).
30028286	4	52	located	observed	328:335	arg1	20-40 °C					340:347	20-40 °C	340:347	20-40 °C (optimum, 25 °C)	340:364	Growth of IB-3T was observed at 20-40 °C (optimum, 25 °C), at pH 6.5-9.0 (optimum, pH 7.5) and in the presence of 0-1 % (w/v) NaCl (optimum, 0 %).
30028286	4	52	located	observed	328:335	arg1	optimum					350:356	optimum	350:356	optimum	350:356	Growth of IB-3T was observed at 20-40 °C (optimum, 25 °C), at pH 6.5-9.0 (optimum, pH 7.5) and in the presence of 0-1 % (w/v) NaCl (optimum, 0 %).
30028286	13	53	theme	type	1446:1449	arg1	IB-3T					1461:1465	IB-3T	1461:1465	IB-3T (=KACC 19522T=JCM 32672T)	1461:1491	The type strain is IB-3T (=KACC 19522T=JCM 32672T).
30028286	13	53	theme	type	1446:1449	arg1	strain					1451:1456	The type strain	1442:1456	The type strain	1442:1456	The type strain is IB-3T (=KACC 19522T=JCM 32672T).
30028286	7	54	theme	cell	838:841	arg1	type					862:865	The major cell wall peptidoglycan type	828:865	The major cell wall peptidoglycan type	828:865	The major cell wall peptidoglycan type was ll-2,6-diaminopimelic acid.
30028286	7	54	theme	cell	838:841	arg1	acid					893:896	ll-2,6-diaminopimelic acid	871:896	ll-2,6-diaminopimelic acid	871:896	The major cell wall peptidoglycan type was ll-2,6-diaminopimelic acid.
30028286	9	55	with	terrigenaDS-17T	996:1010	arg1	similarity					1049:1058	a 98.0 % 16S rRNA gene sequence similarity	1017:1058	a 98.0 % 16S rRNA gene sequence similarity	1017:1058	IB-3T was most closely related to Nocardioides terrigenaDS-17T with a 98.0 % 16S rRNA gene sequence similarity.
30028286	5	56	dep	C17 	587:590	arg1	 0					604:605	 0	604:605	 0	604:605	Menaquinone-8 (H4) was detected as the predominant respiratory quinone and iso-C16 : 0, 10-methyl-C17 : 0, iso-C17 : 0, C18 : 1ω9c, C17 : 1ω8c, C18 : 0, 10-methyl-C18 : 0 (TBSA) and C17 : 0 were identified as the major cellular fatty acids.
30028286	5	56	dep	C17 	587:590	arg1	C18 					599:602	C18 	599:602	C18 	599:602	Menaquinone-8 (H4) was detected as the predominant respiratory quinone and iso-C16 : 0, 10-methyl-C17 : 0, iso-C17 : 0, C18 : 1ω9c, C17 : 1ω8c, C18 : 0, 10-methyl-C18 : 0 (TBSA) and C17 : 0 were identified as the major cellular fatty acids.
30028286	11	57	theme	Nocardioidescurrus	1401:1418	arg1	sp					1420:1421	the name Nocardioidescurrus sp	1392:1421	the name Nocardioidescurrus sp	1392:1421	On the basis of the phenotypic, chemotaxonomic and molecular features, IB-3T represents a novel species of the genus Nocardioides, for which the name Nocardioidescurrus sp.
30028286	3	58	theme	oxidase-positive	280:295	arg1	reactions					297:305	catalase- and oxidase-positive reactions	266:305	catalase- and oxidase-positive reactions	266:305	Cells were non-motile rods showing catalase- and oxidase-positive reactions.
30028286	8	59	theme	71.5 mol	938:945	arg1	G+C content					903:913	The G+C content	899:913	The G+C content of the genomic DNA	899:932	The G+C content of the genomic DNA was 71.5 mol%.
30028286	8	59	theme	71.5 mol	938:945	arg1	%					946:946	71.5 mol%	938:946	71.5 mol%	938:946	The G+C content of the genomic DNA was 71.5 mol%.
30028286	5	60	dep	 1ω9c	580:584	arg1	 0					623:624	 0	623:624	 0 (TBSA)	623:631	Menaquinone-8 (H4) was detected as the predominant respiratory quinone and iso-C16 : 0, 10-methyl-C17 : 0, iso-C17 : 0, C18 : 1ω9c, C17 : 1ω8c, C18 : 0, 10-methyl-C18 : 0 (TBSA) and C17 : 0 were identified as the major cellular fatty acids.
30028286	5	60	dep	 1ω9c	580:584	arg1	TBSA					627:630	TBSA	627:630	TBSA	627:630	Menaquinone-8 (H4) was detected as the predominant respiratory quinone and iso-C16 : 0, 10-methyl-C17 : 0, iso-C17 : 0, C18 : 1ω9c, C17 : 1ω8c, C18 : 0, 10-methyl-C18 : 0 (TBSA) and C17 : 0 were identified as the major cellular fatty acids.
30028286	2	61	theme	air-conditioning	181:196	arg1	system					198:203	a car air-conditioning system	175:203	a car air-conditioning system in the Republic of Korea	175:228	A strictly aerobic Gram-stain-positive bacterial strain, designated IB-3T, was isolated from a car air-conditioning system in the Republic of Korea.
30028286	7	62	theme	major	832:836	arg1	type					862:865	The major cell wall peptidoglycan type	828:865	The major cell wall peptidoglycan type	828:865	The major cell wall peptidoglycan type was ll-2,6-diaminopimelic acid.
30028286	7	62	theme	major	832:836	arg1	acid					893:896	ll-2,6-diaminopimelic acid	871:896	ll-2,6-diaminopimelic acid	871:896	The major cell wall peptidoglycan type was ll-2,6-diaminopimelic acid.
30028286	4	63	theme	IB-3T	318:322	arg1	Growth					308:313	Growth	308:313	Growth of IB-3T	308:322	Growth of IB-3T was observed at 20-40 °C (optimum, 25 °C), at pH 6.5-9.0 (optimum, pH 7.5) and in the presence of 0-1 % (w/v) NaCl (optimum, 0 %).
30028286	11	64	theme	molecular	1302:1310	arg1	features					1312:1319	the phenotypic, chemotaxonomic and molecular features	1267:1319	the phenotypic, chemotaxonomic and molecular features	1267:1319	On the basis of the phenotypic, chemotaxonomic and molecular features, IB-3T represents a novel species of the genus Nocardioides, for which the name Nocardioidescurrus sp.
30028286	9	65	theme	98.0 	1019:1023	arg1	%					1024:1024	%	1024:1024	%	1024:1024	IB-3T was most closely related to Nocardioides terrigenaDS-17T with a 98.0 % 16S rRNA gene sequence similarity.
30028286	2	66	attach	isolated	161:168	arg1	system					198:203	a car air-conditioning system	175:203	a car air-conditioning system in the Republic of Korea	175:228	A strictly aerobic Gram-stain-positive bacterial strain, designated IB-3T, was isolated from a car air-conditioning system in the Republic of Korea.
30028286	2	66	attach	isolated	161:168	arg2	strain					131:136	A strictly aerobic Gram-stain-positive bacterial strain	82:136	A strictly aerobic Gram-stain-positive bacterial strain	82:136	A strictly aerobic Gram-stain-positive bacterial strain, designated IB-3T, was isolated from a car air-conditioning system in the Republic of Korea.
30028286	11	67	theme	novel	1341:1345	arg1	species					1347:1353	a novel species	1339:1353	a novel species	1339:1353	On the basis of the phenotypic, chemotaxonomic and molecular features, IB-3T represents a novel species of the genus Nocardioides, for which the name Nocardioidescurrus sp.
30028286	10	68	dep	Nocardioidesof	1208:1221	arg1	Nocardioidaceae					1234:1248	the family Nocardioidaceae	1223:1248	the genus Nocardioidesof the family Nocardioidaceae	1198:1248	The results of phylogenetic analyses based on 16S rRNA gene sequences indicated that IB-3T formed a distinct phylogenetic lineage within the genus Nocardioidesof the family Nocardioidaceae.
30028286	5	69	dep	C18 	575:578	arg1	10-methyl-C18 					608:621	10-methyl-C18 	608:621	10-methyl-C18 	608:621	Menaquinone-8 (H4) was detected as the predominant respiratory quinone and iso-C16 : 0, 10-methyl-C17 : 0, iso-C17 : 0, C18 : 1ω9c, C17 : 1ω8c, C18 : 0, 10-methyl-C18 : 0 (TBSA) and C17 : 0 were identified as the major cellular fatty acids.
30028286	5	69	dep	C18 	575:578	arg1	 1ω9c					580:584	 1ω9c	580:584	 1ω9c	580:584	Menaquinone-8 (H4) was detected as the predominant respiratory quinone and iso-C16 : 0, 10-methyl-C17 : 0, iso-C17 : 0, C18 : 1ω9c, C17 : 1ω8c, C18 : 0, 10-methyl-C18 : 0 (TBSA) and C17 : 0 were identified as the major cellular fatty acids.
30028286	5	69	dep	C18 	575:578	arg1	C17 					587:590	C17 	587:590	C17 	587:590	Menaquinone-8 (H4) was detected as the predominant respiratory quinone and iso-C16 : 0, 10-methyl-C17 : 0, iso-C17 : 0, C18 : 1ω9c, C17 : 1ω8c, C18 : 0, 10-methyl-C18 : 0 (TBSA) and C17 : 0 were identified as the major cellular fatty acids.
30028286	4	70	theme	w/v	429:431	arg1	optimum					440:446	optimum	440:446	optimum	440:446	Growth of IB-3T was observed at 20-40 °C (optimum, 25 °C), at pH 6.5-9.0 (optimum, pH 7.5) and in the presence of 0-1 % (w/v) NaCl (optimum, 0 %).
30028286	4	70	theme	w/v	429:431	arg1	NaCl					434:437	0-1 % (w/v) NaCl	422:437	0-1 % (w/v) NaCl (optimum, 0 %)	422:452	Growth of IB-3T was observed at 20-40 °C (optimum, 25 °C), at pH 6.5-9.0 (optimum, pH 7.5) and in the presence of 0-1 % (w/v) NaCl (optimum, 0 %).
30028286	5	71	theme	major	668:672	arg1	 0					539:540	 0	539:540	 0	539:540	Menaquinone-8 (H4) was detected as the predominant respiratory quinone and iso-C16 : 0, 10-methyl-C17 : 0, iso-C17 : 0, C18 : 1ω9c, C17 : 1ω8c, C18 : 0, 10-methyl-C18 : 0 (TBSA) and C17 : 0 were identified as the major cellular fatty acids.
30028286	5	71	theme	major	668:672	arg1	acids					689:693	the major cellular fatty acids	664:693	the major cellular fatty acids	664:693	Menaquinone-8 (H4) was detected as the predominant respiratory quinone and iso-C16 : 0, 10-methyl-C17 : 0, iso-C17 : 0, C18 : 1ω9c, C17 : 1ω8c, C18 : 0, 10-methyl-C18 : 0 (TBSA) and C17 : 0 were identified as the major cellular fatty acids.
30028286	10	72	theme	analyses	1089:1096	arg1	results					1065:1071	The results	1061:1071	The results of phylogenetic analyses based on 16S rRNA gene sequences	1061:1129	The results of phylogenetic analyses based on 16S rRNA gene sequences indicated that IB-3T formed a distinct phylogenetic lineage within the genus Nocardioidesof the family Nocardioidaceae.
30028286	4	73	dep	optimum	382:388	arg1	pH					391:392	pH 7.5	391:396	pH 7.5	391:396	Growth of IB-3T was observed at 20-40 °C (optimum, 25 °C), at pH 6.5-9.0 (optimum, pH 7.5) and in the presence of 0-1 % (w/v) NaCl (optimum, 0 %).
31415241	2	0	theme	left	387:390	arg1	samples					402:408	left ventricle samples	387:408	left ventricle samples	387:408	Here, analysis of left ventricle samples showed that CR did not reduce cardiac fibrosis relative to controls.
31415241	5	1	theme	transcriptomics	957:971	arg1	analysis					973:980	transcriptomics analysis	957:980	transcriptomics analysis	957:980	Consistent with the findings from the NHP samples, mice fed a UW or a modified NIA diet with increased sucrose and fat developed greater cardiac fibrosis compared with mice fed the NIA diet, and transcriptomics analysis revealed diet-induced activation of myocardial oxidative phosphorylation and cardiac muscle contraction pathways.
31415241	4	2	theme	Diet	576:579	arg1	difference					609:618	a prominent difference	597:618	a prominent difference between the studies; therefore, we used the NHP diets to characterize diet-associated molecular and functional changes in the hearts of mice	597:759	Diet composition was a prominent difference between the studies; therefore, we used the NHP diets to characterize diet-associated molecular and functional changes in the hearts of mice.
31415241	4	2	theme	Diet	576:579	arg1	composition					581:591	Diet composition	576:591	Diet composition	576:591	Diet composition was a prominent difference between the studies; therefore, we used the NHP diets to characterize diet-associated molecular and functional changes in the hearts of mice.
31415241	5	3	theme	modified	832:839	arg1	diet					845:848	a UW or a modified NIA diet	822:848	diet	845:848	Consistent with the findings from the NHP samples, mice fed a UW or a modified NIA diet with increased sucrose and fat developed greater cardiac fibrosis compared with mice fed the NIA diet, and transcriptomics analysis revealed diet-induced activation of myocardial oxidative phosphorylation and cardiac muscle contraction pathways.
31415241	1	4	theme	health	128:133	arg1	span					135:138	health span	128:138	health span	128:138	Calorie restriction (CR) improved health span in 2 longitudinal studies in nonhuman primates (NHPs), yet only the University of Wisconsin (UW) study demonstrated an increase in survival in CR monkeys relative to controls; the National Institute on Aging (NIA) study did not.
31415241	5	5	theme	greater	891:897	arg1	fibrosis					907:914	greater cardiac fibrosis	891:914	greater cardiac fibrosis	891:914	Consistent with the findings from the NHP samples, mice fed a UW or a modified NIA diet with increased sucrose and fat developed greater cardiac fibrosis compared with mice fed the NIA diet, and transcriptomics analysis revealed diet-induced activation of myocardial oxidative phosphorylation and cardiac muscle contraction pathways.
31415241	5	6	theme	muscle	1067:1072	arg1	contraction					1074:1084	cardiac muscle contraction	1059:1084	cardiac muscle contraction	1059:1084	Consistent with the findings from the NHP samples, mice fed a UW or a modified NIA diet with increased sucrose and fat developed greater cardiac fibrosis compared with mice fed the NIA diet, and transcriptomics analysis revealed diet-induced activation of myocardial oxidative phosphorylation and cardiac muscle contraction pathways.
31415241	3	7	theme	NIA	564:566	arg1	hearts					568:573	NIA hearts	564:573	NIA hearts	564:573	However, there was a 5.9-fold increase of total fibrosis in UW hearts, compared with NIA hearts.
31415241	1	8	from	increase	259:266	arg1	survival					271:278	survival	271:278	survival in CR monkeys relative to controls	271:313	Calorie restriction (CR) improved health span in 2 longitudinal studies in nonhuman primates (NHPs), yet only the University of Wisconsin (UW) study demonstrated an increase in survival in CR monkeys relative to controls; the National Institute on Aging (NIA) study did not.
31415241	3	9	theme	fibrosis	527:534	arg1	increase					509:516	a 5.9-fold increase	498:516	a 5.9-fold increase of total fibrosis in UW hearts	498:547	However, there was a 5.9-fold increase of total fibrosis in UW hearts, compared with NIA hearts.
31415241	5	10	theme	contraction	1074:1084	arg1	pathways					1086:1093	myocardial oxidative phosphorylation and cardiac muscle contraction pathways	1018:1093	myocardial oxidative phosphorylation and cardiac muscle contraction pathways	1018:1093	Consistent with the findings from the NHP samples, mice fed a UW or a modified NIA diet with increased sucrose and fat developed greater cardiac fibrosis compared with mice fed the NIA diet, and transcriptomics analysis revealed diet-induced activation of myocardial oxidative phosphorylation and cardiac muscle contraction pathways.
31415241	0	11	theme	rhesus	78:83	arg1	monkeys					85:91	rhesus monkeys	78:91	rhesus monkeys	78:91	Effect of dietary fat and sucrose consumption on cardiac fibrosis in mice and rhesus monkeys.
31415241	1	12	theme	CR	283:284	arg1	monkeys					286:292	CR monkeys	283:292	CR monkeys relative to controls	283:313	Calorie restriction (CR) improved health span in 2 longitudinal studies in nonhuman primates (NHPs), yet only the University of Wisconsin (UW) study demonstrated an increase in survival in CR monkeys relative to controls; the National Institute on Aging (NIA) study did not.
31415241	4	13	theme	mice	756:759	arg1	hearts					746:751	the hearts	742:751	the hearts of mice	742:759	Diet composition was a prominent difference between the studies; therefore, we used the NHP diets to characterize diet-associated molecular and functional changes in the hearts of mice.
31415241	1	14	theme	University	208:217	arg1	study					237:241	only the University of Wisconsin (UW) study	199:241	only the University of Wisconsin (UW) study	199:241	Calorie restriction (CR) improved health span in 2 longitudinal studies in nonhuman primates (NHPs), yet only the University of Wisconsin (UW) study demonstrated an increase in survival in CR monkeys relative to controls; the National Institute on Aging (NIA) study did not.
31415241	1	15	from	Institute	329:337	arg1	study					354:358	Aging (NIA) study	342:358	Aging (NIA) study	342:358	Calorie restriction (CR) improved health span in 2 longitudinal studies in nonhuman primates (NHPs), yet only the University of Wisconsin (UW) study demonstrated an increase in survival in CR monkeys relative to controls; the National Institute on Aging (NIA) study did not.
31415241	5	16	with	Consistent	762:771	arg1	findings					782:789	the findings	778:789	the findings from the NHP samples	778:810	Consistent with the findings from the NHP samples, mice fed a UW or a modified NIA diet with increased sucrose and fat developed greater cardiac fibrosis compared with mice fed the NIA diet, and transcriptomics analysis revealed diet-induced activation of myocardial oxidative phosphorylation and cardiac muscle contraction pathways.
31415241	4	17	used	used	655:658	arg2	we					652:653	we	652:653	we	652:653	Diet composition was a prominent difference between the studies; therefore, we used the NHP diets to characterize diet-associated molecular and functional changes in the hearts of mice.
31415241	1	18	theme	relative	294:301	arg1	monkeys					286:292	CR monkeys	283:292	CR monkeys relative to controls	283:313	Calorie restriction (CR) improved health span in 2 longitudinal studies in nonhuman primates (NHPs), yet only the University of Wisconsin (UW) study demonstrated an increase in survival in CR monkeys relative to controls; the National Institute on Aging (NIA) study did not.
31415241	4	19	theme	functional	720:729	arg1	changes					731:737	diet-associated molecular and functional changes	690:737	diet-associated molecular and functional changes in the hearts of mice	690:759	Diet composition was a prominent difference between the studies; therefore, we used the NHP diets to characterize diet-associated molecular and functional changes in the hearts of mice.
31415241	5	20	theme	pathways	1086:1093	arg1	activation					1004:1013	diet-induced activation	991:1013	diet-induced activation of myocardial oxidative phosphorylation and cardiac muscle contraction pathways	991:1093	Consistent with the findings from the NHP samples, mice fed a UW or a modified NIA diet with increased sucrose and fat developed greater cardiac fibrosis compared with mice fed the NIA diet, and transcriptomics analysis revealed diet-induced activation of myocardial oxidative phosphorylation and cardiac muscle contraction pathways.
31415241	0	21	theme	consumption	34:44	arg1	Effect					0:5	Effect	0:5	Effect of dietary fat and sucrose consumption on cardiac fibrosis in mice and rhesus monkeys.	0:92	Effect of dietary fat and sucrose consumption on cardiac fibrosis in mice and rhesus monkeys.
31415241	3	22	from	increase	509:516	arg1	hearts					542:547	UW hearts	539:547	UW hearts	539:547	However, there was a 5.9-fold increase of total fibrosis in UW hearts, compared with NIA hearts.
31415241	4	23	dep	studies	632:638	arg1	used					655:658	used	655:658	used the NHP diets to characterize diet-associated molecular and functional changes in the hearts of mice	655:759	Diet composition was a prominent difference between the studies; therefore, we used the NHP diets to characterize diet-associated molecular and functional changes in the hearts of mice.
31415241	4	24	from	changes	731:737	arg1	hearts					746:751	the hearts	742:751	the hearts of mice	742:759	Diet composition was a prominent difference between the studies; therefore, we used the NHP diets to characterize diet-associated molecular and functional changes in the hearts of mice.
31415241	1	25	theme	longitudinal	145:156	arg1	studies					158:164	2 longitudinal studies	143:164	2 longitudinal studies in nonhuman primates (NHPs)	143:192	Calorie restriction (CR) improved health span in 2 longitudinal studies in nonhuman primates (NHPs), yet only the University of Wisconsin (UW) study demonstrated an increase in survival in CR monkeys relative to controls; the National Institute on Aging (NIA) study did not.
31415241	5	26	theme	oxidative	1029:1037	arg1	phosphorylation					1039:1053	myocardial oxidative phosphorylation	1018:1053	myocardial oxidative phosphorylation	1018:1053	Consistent with the findings from the NHP samples, mice fed a UW or a modified NIA diet with increased sucrose and fat developed greater cardiac fibrosis compared with mice fed the NIA diet, and transcriptomics analysis revealed diet-induced activation of myocardial oxidative phosphorylation and cardiac muscle contraction pathways.
31415241	0	27	theme	fat	18:20	arg1	Effect					0:5	Effect	0:5	Effect of dietary fat and sucrose consumption on cardiac fibrosis in mice and rhesus monkeys.	0:92	Effect of dietary fat and sucrose consumption on cardiac fibrosis in mice and rhesus monkeys.
31415241	5	28	theme	phosphorylation	1039:1053	arg1	pathways					1086:1093	myocardial oxidative phosphorylation and cardiac muscle contraction pathways	1018:1093	myocardial oxidative phosphorylation and cardiac muscle contraction pathways	1018:1093	Consistent with the findings from the NHP samples, mice fed a UW or a modified NIA diet with increased sucrose and fat developed greater cardiac fibrosis compared with mice fed the NIA diet, and transcriptomics analysis revealed diet-induced activation of myocardial oxidative phosphorylation and cardiac muscle contraction pathways.
31415241	0	29	theme	dietary	10:16	arg1	fat					18:20	dietary fat	10:20	dietary fat	10:20	Effect of dietary fat and sucrose consumption on cardiac fibrosis in mice and rhesus monkeys.
31415241	2	30	theme	cardiac	440:446	arg1	fibrosis					448:455	cardiac fibrosis	440:455	cardiac fibrosis relative to controls	440:476	Here, analysis of left ventricle samples showed that CR did not reduce cardiac fibrosis relative to controls.
31415241	3	31	theme	total	521:525	arg1	fibrosis					527:534	total fibrosis	521:534	total fibrosis	521:534	However, there was a 5.9-fold increase of total fibrosis in UW hearts, compared with NIA hearts.
31415241	1	32	theme	nonhuman	169:176	arg1	NHPs					188:191	NHPs	188:191	NHPs	188:191	Calorie restriction (CR) improved health span in 2 longitudinal studies in nonhuman primates (NHPs), yet only the University of Wisconsin (UW) study demonstrated an increase in survival in CR monkeys relative to controls; the National Institute on Aging (NIA) study did not.
31415241	1	32	theme	nonhuman	169:176	arg1	primates					178:185	nonhuman primates	169:185	nonhuman primates (NHPs)	169:192	Calorie restriction (CR) improved health span in 2 longitudinal studies in nonhuman primates (NHPs), yet only the University of Wisconsin (UW) study demonstrated an increase in survival in CR monkeys relative to controls; the National Institute on Aging (NIA) study did not.
31415241	0	33	theme	sucrose	26:32	arg1	consumption					34:44	sucrose consumption	26:44	sucrose consumption	26:44	Effect of dietary fat and sucrose consumption on cardiac fibrosis in mice and rhesus monkeys.
31415241	5	34	theme	cardiac	1059:1065	arg1	contraction					1074:1084	cardiac muscle contraction	1059:1084	cardiac muscle contraction	1059:1084	Consistent with the findings from the NHP samples, mice fed a UW or a modified NIA diet with increased sucrose and fat developed greater cardiac fibrosis compared with mice fed the NIA diet, and transcriptomics analysis revealed diet-induced activation of myocardial oxidative phosphorylation and cardiac muscle contraction pathways.
31415241	3	35	theme	UW	539:540	arg1	hearts					542:547	UW hearts	539:547	UW hearts	539:547	However, there was a 5.9-fold increase of total fibrosis in UW hearts, compared with NIA hearts.
31415241	5	36	theme	increased	855:863	arg1	sucrose					865:871	increased sucrose	855:871	increased sucrose	855:871	Consistent with the findings from the NHP samples, mice fed a UW or a modified NIA diet with increased sucrose and fat developed greater cardiac fibrosis compared with mice fed the NIA diet, and transcriptomics analysis revealed diet-induced activation of myocardial oxidative phosphorylation and cardiac muscle contraction pathways.
31415241	5	37	theme	diet-induced	991:1002	arg1	activation					1004:1013	diet-induced activation	991:1013	diet-induced activation of myocardial oxidative phosphorylation and cardiac muscle contraction pathways	991:1093	Consistent with the findings from the NHP samples, mice fed a UW or a modified NIA diet with increased sucrose and fat developed greater cardiac fibrosis compared with mice fed the NIA diet, and transcriptomics analysis revealed diet-induced activation of myocardial oxidative phosphorylation and cardiac muscle contraction pathways.
31415241	1	38	theme	National	320:327	arg1	Institute					329:337	the National Institute	316:337	the National Institute on Aging (NIA) study	316:358	Calorie restriction (CR) improved health span in 2 longitudinal studies in nonhuman primates (NHPs), yet only the University of Wisconsin (UW) study demonstrated an increase in survival in CR monkeys relative to controls; the National Institute on Aging (NIA) study did not.
31415241	5	39	theme	myocardial	1018:1027	arg1	phosphorylation					1039:1053	myocardial oxidative phosphorylation	1018:1053	myocardial oxidative phosphorylation	1018:1053	Consistent with the findings from the NHP samples, mice fed a UW or a modified NIA diet with increased sucrose and fat developed greater cardiac fibrosis compared with mice fed the NIA diet, and transcriptomics analysis revealed diet-induced activation of myocardial oxidative phosphorylation and cardiac muscle contraction pathways.
31415241	2	40	theme	ventricle	392:400	arg1	samples					402:408	left ventricle samples	387:408	left ventricle samples	387:408	Here, analysis of left ventricle samples showed that CR did not reduce cardiac fibrosis relative to controls.
31415241	4	41	theme	molecular	706:714	arg1	changes					731:737	diet-associated molecular and functional changes	690:737	diet-associated molecular and functional changes in the hearts of mice	690:759	Diet composition was a prominent difference between the studies; therefore, we used the NHP diets to characterize diet-associated molecular and functional changes in the hearts of mice.
31415241	1	42	theme	Calorie	94:100	arg1	CR					115:116	CR	115:116	CR	115:116	Calorie restriction (CR) improved health span in 2 longitudinal studies in nonhuman primates (NHPs), yet only the University of Wisconsin (UW) study demonstrated an increase in survival in CR monkeys relative to controls; the National Institute on Aging (NIA) study did not.
31415241	1	42	theme	Calorie	94:100	arg1	restriction					102:112	Calorie restriction	94:112	Calorie restriction (CR)	94:117	Calorie restriction (CR) improved health span in 2 longitudinal studies in nonhuman primates (NHPs), yet only the University of Wisconsin (UW) study demonstrated an increase in survival in CR monkeys relative to controls; the National Institute on Aging (NIA) study did not.
31415241	1	43	from	survival	271:278	arg1	monkeys					286:292	CR monkeys	283:292	CR monkeys relative to controls	283:313	Calorie restriction (CR) improved health span in 2 longitudinal studies in nonhuman primates (NHPs), yet only the University of Wisconsin (UW) study demonstrated an increase in survival in CR monkeys relative to controls; the National Institute on Aging (NIA) study did not.
31415241	4	44	theme	diet-associated	690:704	arg1	changes					731:737	diet-associated molecular and functional changes	690:737	diet-associated molecular and functional changes in the hearts of mice	690:759	Diet composition was a prominent difference between the studies; therefore, we used the NHP diets to characterize diet-associated molecular and functional changes in the hearts of mice.
31415241	0	45	from	fibrosis	57:64	arg1	mice					69:72	mice	69:72	mice	69:72	Effect of dietary fat and sucrose consumption on cardiac fibrosis in mice and rhesus monkeys.
31415241	0	45	from	fibrosis	57:64	arg1	monkeys					85:91	rhesus monkeys	78:91	rhesus monkeys	78:91	Effect of dietary fat and sucrose consumption on cardiac fibrosis in mice and rhesus monkeys.
31415241	4	46	theme	NHP	664:666	arg1	diets					668:672	the NHP diets	660:672	the NHP diets	660:672	Diet composition was a prominent difference between the studies; therefore, we used the NHP diets to characterize diet-associated molecular and functional changes in the hearts of mice.
31415241	1	47	theme	Aging	342:346	arg1	study					354:358	Aging (NIA) study	342:358	Aging (NIA) study	342:358	Calorie restriction (CR) improved health span in 2 longitudinal studies in nonhuman primates (NHPs), yet only the University of Wisconsin (UW) study demonstrated an increase in survival in CR monkeys relative to controls; the National Institute on Aging (NIA) study did not.
31415241	5	48	theme	NIA	943:945	arg1	diet					947:950	the NIA diet	939:950	the NIA diet	939:950	Consistent with the findings from the NHP samples, mice fed a UW or a modified NIA diet with increased sucrose and fat developed greater cardiac fibrosis compared with mice fed the NIA diet, and transcriptomics analysis revealed diet-induced activation of myocardial oxidative phosphorylation and cardiac muscle contraction pathways.
31415241	4	49	theme	prominent	599:607	arg1	difference					609:618	a prominent difference	597:618	a prominent difference between the studies; therefore, we used the NHP diets to characterize diet-associated molecular and functional changes in the hearts of mice	597:759	Diet composition was a prominent difference between the studies; therefore, we used the NHP diets to characterize diet-associated molecular and functional changes in the hearts of mice.
31415241	4	49	theme	prominent	599:607	arg1	composition					581:591	Diet composition	576:591	Diet composition	576:591	Diet composition was a prominent difference between the studies; therefore, we used the NHP diets to characterize diet-associated molecular and functional changes in the hearts of mice.
31415241	2	50	theme	relative	457:464	arg1	fibrosis					448:455	cardiac fibrosis	440:455	cardiac fibrosis relative to controls	440:476	Here, analysis of left ventricle samples showed that CR did not reduce cardiac fibrosis relative to controls.
31415241	5	51	theme	cardiac	899:905	arg1	fibrosis					907:914	greater cardiac fibrosis	891:914	greater cardiac fibrosis	891:914	Consistent with the findings from the NHP samples, mice fed a UW or a modified NIA diet with increased sucrose and fat developed greater cardiac fibrosis compared with mice fed the NIA diet, and transcriptomics analysis revealed diet-induced activation of myocardial oxidative phosphorylation and cardiac muscle contraction pathways.
31415241	5	52	theme	NIA	841:843	arg1	diet					845:848	a UW or a modified NIA diet	822:848	diet	845:848	Consistent with the findings from the NHP samples, mice fed a UW or a modified NIA diet with increased sucrose and fat developed greater cardiac fibrosis compared with mice fed the NIA diet, and transcriptomics analysis revealed diet-induced activation of myocardial oxidative phosphorylation and cardiac muscle contraction pathways.
31415241	5	53	theme	NHP	800:802	arg1	samples					804:810	the NHP samples	796:810	the NHP samples	796:810	Consistent with the findings from the NHP samples, mice fed a UW or a modified NIA diet with increased sucrose and fat developed greater cardiac fibrosis compared with mice fed the NIA diet, and transcriptomics analysis revealed diet-induced activation of myocardial oxidative phosphorylation and cardiac muscle contraction pathways.
31415241	5	54	from	samples	804:810	arg1	findings					782:789	the findings	778:789	the findings from the NHP samples	778:810	Consistent with the findings from the NHP samples, mice fed a UW or a modified NIA diet with increased sucrose and fat developed greater cardiac fibrosis compared with mice fed the NIA diet, and transcriptomics analysis revealed diet-induced activation of myocardial oxidative phosphorylation and cardiac muscle contraction pathways.
31415241	2	55	theme	samples	402:408	arg1	analysis					375:382	analysis	375:382	analysis of left ventricle samples	375:408	Here, analysis of left ventricle samples showed that CR did not reduce cardiac fibrosis relative to controls.
31415241	1	56	theme	NIA	349:351	arg1	study					354:358	Aging (NIA) study	342:358	Aging (NIA) study	342:358	Calorie restriction (CR) improved health span in 2 longitudinal studies in nonhuman primates (NHPs), yet only the University of Wisconsin (UW) study demonstrated an increase in survival in CR monkeys relative to controls; the National Institute on Aging (NIA) study did not.
31415241	1	57	from	studies	158:164	arg1	NHPs					188:191	NHPs	188:191	NHPs	188:191	Calorie restriction (CR) improved health span in 2 longitudinal studies in nonhuman primates (NHPs), yet only the University of Wisconsin (UW) study demonstrated an increase in survival in CR monkeys relative to controls; the National Institute on Aging (NIA) study did not.
31415241	1	57	from	studies	158:164	arg1	primates					178:185	nonhuman primates	169:185	nonhuman primates (NHPs)	169:192	Calorie restriction (CR) improved health span in 2 longitudinal studies in nonhuman primates (NHPs), yet only the University of Wisconsin (UW) study demonstrated an increase in survival in CR monkeys relative to controls; the National Institute on Aging (NIA) study did not.
31415241	0	58	theme	cardiac	49:55	arg1	fibrosis					57:64	cardiac fibrosis	49:64	cardiac fibrosis in mice and rhesus monkeys	49:91	Effect of dietary fat and sucrose consumption on cardiac fibrosis in mice and rhesus monkeys.
31415241	3	59	theme	5.9-fold	500:507	arg1	increase					509:516	a 5.9-fold increase	498:516	a 5.9-fold increase of total fibrosis in UW hearts	498:547	However, there was a 5.9-fold increase of total fibrosis in UW hearts, compared with NIA hearts.
31415241	0	60	from	Effect	0:5	arg1	fibrosis					57:64	cardiac fibrosis	49:64	cardiac fibrosis in mice and rhesus monkeys	49:91	Effect of dietary fat and sucrose consumption on cardiac fibrosis in mice and rhesus monkeys.
30811968	10	0	theme	disc	1396:1399	arg1	composition					1362:1372	collagen composition	1353:1372	collagen composition of the intervertebral disc	1353:1399	The cKO mice exhibited changes in functional attributes of aggrecan as well as in collagen composition of the intervertebral disc.
30811968	7	1	theme	reporter	906:913	arg1	mice					915:918	Quantitative histology, gene expression and a novel p16tdTom reporter mice	845:918	Quantitative histology, gene expression and a novel p16tdTom reporter mice	845:918	Quantitative histology, gene expression and a novel p16tdTom reporter mice showed an increase in p16Ink4a, p21 and IL-6, with a decrease in Ki67 with aging.
30811968	12	2	dep	markers	1651:1657	arg1	CA3					1659:1661	CA3	1659:1661	CA3	1659:1661	The cKO mice maintained expression of NP-cell phenotypic markers CA3, Krt19 and GLUT-1.
30811968	12	2	dep	markers	1651:1657	arg1	GLUT-1					1674:1679	GLUT-1	1674:1679	GLUT-1	1674:1679	The cKO mice maintained expression of NP-cell phenotypic markers CA3, Krt19 and GLUT-1.
30811968	12	2	dep	markers	1651:1657	arg1	markers					1651:1657	NP-cell phenotypic markers CA3, Krt19 and GLUT-1	1632:1679	NP-cell phenotypic markers CA3, Krt19 and GLUT-1	1632:1679	The cKO mice maintained expression of NP-cell phenotypic markers CA3, Krt19 and GLUT-1.
30811968	12	2	dep	markers	1651:1657	arg1	Krt19					1664:1668	Krt19	1664:1668	Krt19	1664:1668	The cKO mice maintained expression of NP-cell phenotypic markers CA3, Krt19 and GLUT-1.
30811968	4	3	theme	degenerative	608:619	arg1	phenotype					621:629	the degenerative phenotype	604:629	the degenerative phenotype in many age-associated diseases	604:661	Clearance of p16Ink4a-positive senescent cells reduces the degenerative phenotype in many age-associated diseases.
30811968	2	4	theme	genetic	299:305	arg1	factors					307:313	many environmental and genetic factors	276:313	many environmental and genetic factors	276:313	Although many environmental and genetic factors are known to contribute to disc degeneration, age is still the most significant risk factor.
30811968	7	5	theme	novel	891:895	arg1	reporter					906:913	a novel p16tdTom reporter	889:913	a novel p16tdTom reporter	889:913	Quantitative histology, gene expression and a novel p16tdTom reporter mice showed an increase in p16Ink4a, p21 and IL-6, with a decrease in Ki67 with aging.
30811968	9	6	from	change	1220:1225	arg1	morphology					1235:1244	cell morphology	1230:1244	cell morphology	1230:1244	The analyses of discs of cKO and age-matched control mice showed little change in cell morphology and tissue architecture.
30811968	9	6	from	change	1220:1225	arg1	architecture					1257:1268	tissue architecture	1250:1268	tissue architecture	1250:1268	The analyses of discs of cKO and age-matched control mice showed little change in cell morphology and tissue architecture.
30811968	15	7	theme	cells	2118:2122	arg1	homeostasis					2098:2108	matrix homeostasis	2091:2108	matrix homeostasis of disc cells	2091:2122	These results show that while, p16Ink4a is dispensable for induction and maintenance of senescence, conditional loss of p16Ink4a reduces apoptosis, limits the SASP phenotype and alters matrix homeostasis of disc cells.
30811968	9	8	theme	control	1193:1199	arg1	mice					1201:1204	age-matched control mice	1181:1204	age-matched control mice	1181:1204	The analyses of discs of cKO and age-matched control mice showed little change in cell morphology and tissue architecture.
30811968	2	9	theme	environmental	281:293	arg1	factors					307:313	many environmental and genetic factors	276:313	many environmental and genetic factors	276:313	Although many environmental and genetic factors are known to contribute to disc degeneration, age is still the most significant risk factor.
30811968	14	10	theme	markers	1867:1873	arg1	levels					1852:1857	lower levels	1846:1857	lower levels of SASP markers, IL-1β, IL-6, MCP1 and TGF-β1	1846:1903	Interestingly, the cKO discs showed lower levels of SASP markers, IL-1β, IL-6, MCP1 and TGF-β1.
30811968	13	11	theme	RB	1727:1728	arg1	levels					1706:1711	levels	1706:1711	levels of p19Arf and RB	1706:1728	Moreover, in cKO discs, levels of p19Arf and RB were higher without alterations in Ki67, γH2AX, CDK4 and Lipofuscin deposition.
30811968	3	12	from	degeneration	486:497	arg1	models					541:546	mouse models	535:546	mouse models	535:546	Recent studies have shown that senescence may play a role in age-related disc degeneration and matrix catabolism in humans and mouse models.
30811968	3	12	from	degeneration	486:497	arg1	humans					524:529	humans	524:529	humans	524:529	Recent studies have shown that senescence may play a role in age-related disc degeneration and matrix catabolism in humans and mouse models.
30811968	7	13	theme	gene	869:872	arg1	expression					874:883	gene expression	869:883	gene expression	869:883	Quantitative histology, gene expression and a novel p16tdTom reporter mice showed an increase in p16Ink4a, p21 and IL-6, with a decrease in Ki67 with aging.
30811968	15	14	theme	matrix	2091:2096	arg1	homeostasis					2098:2108	matrix homeostasis	2091:2108	matrix homeostasis of disc cells	2091:2122	These results show that while, p16Ink4a is dispensable for induction and maintenance of senescence, conditional loss of p16Ink4a reduces apoptosis, limits the SASP phenotype and alters matrix homeostasis of disc cells.
30811968	11	15	theme	overall	1540:1546	arg1	minimal					1585:1591	minimal	1585:1591	minimal	1585:1591	While cKO discs exhibited a small decrease in TUNEL positive cells, lineage tracing experiments using ZsGreen reporter indicated that the overall changes in cell fate or numbers were minimal.
30811968	11	15	theme	overall	1540:1546	arg1	changes					1548:1554	the overall changes	1536:1554	the overall changes in cell fate or numbers	1536:1578	While cKO discs exhibited a small decrease in TUNEL positive cells, lineage tracing experiments using ZsGreen reporter indicated that the overall changes in cell fate or numbers were minimal.
30811968	7	16	theme	histology	858:866	arg1	mice					915:918	Quantitative histology, gene expression and a novel p16tdTom reporter mice	845:918	Quantitative histology, gene expression and a novel p16tdTom reporter mice	845:918	Quantitative histology, gene expression and a novel p16tdTom reporter mice showed an increase in p16Ink4a, p21 and IL-6, with a decrease in Ki67 with aging.
30811968	14	17	theme	lower	1846:1850	arg1	levels					1852:1857	lower levels	1846:1857	lower levels of SASP markers, IL-1β, IL-6, MCP1 and TGF-β1	1846:1903	Interestingly, the cKO discs showed lower levels of SASP markers, IL-1β, IL-6, MCP1 and TGF-β1.
30811968	9	18	theme	cell	1230:1233	arg1	morphology					1235:1244	cell morphology	1230:1244	cell morphology	1230:1244	The analyses of discs of cKO and age-matched control mice showed little change in cell morphology and tissue architecture.
30811968	15	19	theme	SASP	2065:2068	arg1	phenotype					2070:2078	the SASP phenotype	2061:2078	the SASP phenotype	2061:2078	These results show that while, p16Ink4a is dispensable for induction and maintenance of senescence, conditional loss of p16Ink4a reduces apoptosis, limits the SASP phenotype and alters matrix homeostasis of disc cells.
30811968	13	20	theme	p19Arf	1716:1721	arg1	levels					1706:1711	levels	1706:1711	levels of p19Arf and RB	1706:1728	Moreover, in cKO discs, levels of p19Arf and RB were higher without alterations in Ki67, γH2AX, CDK4 and Lipofuscin deposition.
30811968	5	21	theme	intervertebral	708:721	arg1	degeneration					728:739	intervertebral disc degeneration	708:739	intervertebral disc degeneration	708:739	Whether p16Ink4a plays a functional role in intervertebral disc degeneration and senescence is unknown.
30811968	10	22	theme	intervertebral	1381:1394	arg1	disc					1396:1399	the intervertebral disc	1377:1399	the intervertebral disc	1377:1399	The cKO mice exhibited changes in functional attributes of aggrecan as well as in collagen composition of the intervertebral disc.
30811968	13	23	theme	Lipofuscin	1787:1796	arg1	deposition					1798:1807	Lipofuscin deposition	1787:1807	Lipofuscin deposition	1787:1807	Moreover, in cKO discs, levels of p19Arf and RB were higher without alterations in Ki67, γH2AX, CDK4 and Lipofuscin deposition.
30811968	11	24	theme	lineage	1470:1476	arg1	experiments					1486:1496	lineage tracing experiments	1470:1496	lineage tracing experiments using ZsGreen reporter	1470:1519	While cKO discs exhibited a small decrease in TUNEL positive cells, lineage tracing experiments using ZsGreen reporter indicated that the overall changes in cell fate or numbers were minimal.
30811968	7	25	from	decrease	973:980	arg1	Ki67					985:988	Ki67	985:988	Ki67	985:988	Quantitative histology, gene expression and a novel p16tdTom reporter mice showed an increase in p16Ink4a, p21 and IL-6, with a decrease in Ki67 with aging.
30811968	0	26	theme	matrix	68:73	arg1	homeostasis					75:85	their matrix homeostasis	62:85	their matrix homeostasis	62:85	p16Ink4a deletion in cells of the intervertebral disc affects their matrix homeostasis and senescence associated secretory phenotype without altering onset of senescence.
30811968	1	27	theme	Intervertebral	171:184	arg1	contributor					220:230	an important contributor	207:230	an important contributor to chronic low back and neck pain	207:264	Intervertebral disc degeneration is an important contributor to chronic low back and neck pain.
30811968	1	27	theme	Intervertebral	171:184	arg1	degeneration					191:202	Intervertebral disc degeneration	171:202	Intervertebral disc degeneration	171:202	Intervertebral disc degeneration is an important contributor to chronic low back and neck pain.
30811968	13	28	theme	cKO	1695:1697	arg1	discs					1699:1703	cKO discs	1695:1703	cKO discs	1695:1703	Moreover, in cKO discs, levels of p19Arf and RB were higher without alterations in Ki67, γH2AX, CDK4 and Lipofuscin deposition.
30811968	11	29	theme	ZsGreen	1504:1510	arg1	reporter					1512:1519	ZsGreen reporter	1504:1519	ZsGreen reporter	1504:1519	While cKO discs exhibited a small decrease in TUNEL positive cells, lineage tracing experiments using ZsGreen reporter indicated that the overall changes in cell fate or numbers were minimal.
30811968	0	30	theme	associated	102:111	arg1	phenotype					123:131	associated secretory phenotype	102:131	associated secretory phenotype	102:131	p16Ink4a deletion in cells of the intervertebral disc affects their matrix homeostasis and senescence associated secretory phenotype without altering onset of senescence.
30811968	8	31	theme	mice	1063:1066	arg1	spinal-phenotype					1030:1045	the spinal-phenotype	1026:1045	the spinal-phenotype of 18-month-old mice with conditional deletion of p16Ink4a in the disc driven by Acan-CreERT2 (cKO)	1026:1145	Accordingly, we studied the spinal-phenotype of 18-month-old mice with conditional deletion of p16Ink4a in the disc driven by Acan-CreERT2 (cKO).
30811968	7	32	from	increase	930:937	arg1	IL-6					960:963	IL-6	960:963	IL-6	960:963	Quantitative histology, gene expression and a novel p16tdTom reporter mice showed an increase in p16Ink4a, p21 and IL-6, with a decrease in Ki67 with aging.
30811968	7	32	from	increase	930:937	arg1	p21					952:954	p21	952:954	p21	952:954	Quantitative histology, gene expression and a novel p16tdTom reporter mice showed an increase in p16Ink4a, p21 and IL-6, with a decrease in Ki67 with aging.
30811968	7	32	from	increase	930:937	arg1	p16Ink4a					942:949	p16Ink4a	942:949	p16Ink4a	942:949	Quantitative histology, gene expression and a novel p16tdTom reporter mice showed an increase in p16Ink4a, p21 and IL-6, with a decrease in Ki67 with aging.
30811968	2	33	theme	significant	383:393	arg1	factor					400:405	the most significant risk factor	374:405	the most significant risk factor	374:405	Although many environmental and genetic factors are known to contribute to disc degeneration, age is still the most significant risk factor.
30811968	2	33	theme	significant	383:393	arg1	age					361:363	age	361:363	age	361:363	Although many environmental and genetic factors are known to contribute to disc degeneration, age is still the most significant risk factor.
30811968	0	34	theme	p16Ink4a	0:7	arg1	deletion					9:16	p16Ink4a deletion	0:16	p16Ink4a deletion in cells of the intervertebral disc	0:52	p16Ink4a deletion in cells of the intervertebral disc affects their matrix homeostasis and senescence associated secretory phenotype without altering onset of senescence.
30811968	8	35	theme	conditional	1073:1083	arg1	deletion					1085:1092	conditional deletion	1073:1092	conditional deletion of p16Ink4a in the disc driven by Acan-CreERT2 (cKO)	1073:1145	Accordingly, we studied the spinal-phenotype of 18-month-old mice with conditional deletion of p16Ink4a in the disc driven by Acan-CreERT2 (cKO).
30811968	9	36	theme	age-matched	1181:1191	arg1	mice					1201:1204	age-matched control mice	1181:1204	age-matched control mice	1181:1204	The analyses of discs of cKO and age-matched control mice showed little change in cell morphology and tissue architecture.
30811968	9	37	theme	cKO	1173:1175	arg1	discs					1164:1168	discs	1164:1168	discs of cKO and age-matched control mice	1164:1204	The analyses of discs of cKO and age-matched control mice showed little change in cell morphology and tissue architecture.
30811968	10	38	theme	functional	1305:1314	arg1	attributes					1316:1325	functional attributes	1305:1325	functional attributes of aggrecan	1305:1337	The cKO mice exhibited changes in functional attributes of aggrecan as well as in collagen composition of the intervertebral disc.
30811968	12	39	theme	phenotypic	1640:1649	arg1	CA3					1659:1661	CA3	1659:1661	CA3	1659:1661	The cKO mice maintained expression of NP-cell phenotypic markers CA3, Krt19 and GLUT-1.
30811968	12	39	theme	phenotypic	1640:1649	arg1	GLUT-1					1674:1679	GLUT-1	1674:1679	GLUT-1	1674:1679	The cKO mice maintained expression of NP-cell phenotypic markers CA3, Krt19 and GLUT-1.
30811968	12	39	theme	phenotypic	1640:1649	arg1	markers					1651:1657	NP-cell phenotypic markers CA3, Krt19 and GLUT-1	1632:1679	NP-cell phenotypic markers CA3, Krt19 and GLUT-1	1632:1679	The cKO mice maintained expression of NP-cell phenotypic markers CA3, Krt19 and GLUT-1.
30811968	12	39	theme	phenotypic	1640:1649	arg1	Krt19					1664:1668	Krt19	1664:1668	Krt19	1664:1668	The cKO mice maintained expression of NP-cell phenotypic markers CA3, Krt19 and GLUT-1.
30811968	4	40	theme	p16Ink4a-positive	562:578	arg1	cells					590:594	p16Ink4a-positive senescent cells	562:594	p16Ink4a-positive senescent cells	562:594	Clearance of p16Ink4a-positive senescent cells reduces the degenerative phenotype in many age-associated diseases.
30811968	0	41	theme	intervertebral	34:47	arg1	disc					49:52	the intervertebral disc	30:52	the intervertebral disc	30:52	p16Ink4a deletion in cells of the intervertebral disc affects their matrix homeostasis and senescence associated secretory phenotype without altering onset of senescence.
30811968	2	42	theme	disc	342:345	arg1	degeneration					347:358	disc degeneration	342:358	disc degeneration	342:358	Although many environmental and genetic factors are known to contribute to disc degeneration, age is still the most significant risk factor.
30811968	3	43	theme	mouse	535:539	arg1	models					541:546	mouse models	535:546	mouse models	535:546	Recent studies have shown that senescence may play a role in age-related disc degeneration and matrix catabolism in humans and mouse models.
30811968	15	44	theme	p16Ink4a	2026:2033	arg1	loss					2018:2021	conditional loss	2006:2021	conditional loss of p16Ink4a	2006:2033	These results show that while, p16Ink4a is dispensable for induction and maintenance of senescence, conditional loss of p16Ink4a reduces apoptosis, limits the SASP phenotype and alters matrix homeostasis of disc cells.
30811968	6	45	from	status	806:811	arg1	mice					839:842	young and old mice	825:842	young and old mice	825:842	We first characterized the senescence status of discs in young and old mice.
30811968	10	46	theme	collagen	1353:1360	arg1	composition					1362:1372	collagen composition	1353:1372	collagen composition of the intervertebral disc	1353:1399	The cKO mice exhibited changes in functional attributes of aggrecan as well as in collagen composition of the intervertebral disc.
30811968	11	47	from	decrease	1436:1443	arg1	cells					1463:1467	TUNEL positive cells	1448:1467	TUNEL positive cells	1448:1467	While cKO discs exhibited a small decrease in TUNEL positive cells, lineage tracing experiments using ZsGreen reporter indicated that the overall changes in cell fate or numbers were minimal.
30811968	4	48	theme	many	634:637	arg1	diseases					654:661	many age-associated diseases	634:661	many age-associated diseases	634:661	Clearance of p16Ink4a-positive senescent cells reduces the degenerative phenotype in many age-associated diseases.
30811968	9	49	theme	discs	1164:1168	arg1	analyses					1152:1159	The analyses	1148:1159	The analyses of discs of cKO and age-matched control mice	1148:1204	The analyses of discs of cKO and age-matched control mice showed little change in cell morphology and tissue architecture.
30811968	3	50	theme	matrix	503:508	arg1	catabolism					510:519	matrix catabolism	503:519	matrix catabolism	503:519	Recent studies have shown that senescence may play a role in age-related disc degeneration and matrix catabolism in humans and mouse models.
30811968	4	51	theme	cells	590:594	arg1	Clearance					549:557	Clearance	549:557	Clearance of p16Ink4a-positive senescent cells	549:594	Clearance of p16Ink4a-positive senescent cells reduces the degenerative phenotype in many age-associated diseases.
30811968	11	52	theme	cKO	1408:1410	arg1	discs					1412:1416	cKO discs	1408:1416	cKO discs	1408:1416	While cKO discs exhibited a small decrease in TUNEL positive cells, lineage tracing experiments using ZsGreen reporter indicated that the overall changes in cell fate or numbers were minimal.
30811968	6	53	theme	young	825:829	arg1	mice					839:842	young and old mice	825:842	young and old mice	825:842	We first characterized the senescence status of discs in young and old mice.
30811968	5	54	theme	functional	689:698	arg1	role					700:703	a functional role	687:703	a functional role	687:703	Whether p16Ink4a plays a functional role in intervertebral disc degeneration and senescence is unknown.
30811968	3	55	from	catabolism	510:519	arg1	models					541:546	mouse models	535:546	mouse models	535:546	Recent studies have shown that senescence may play a role in age-related disc degeneration and matrix catabolism in humans and mouse models.
30811968	3	55	from	catabolism	510:519	arg1	humans					524:529	humans	524:529	humans	524:529	Recent studies have shown that senescence may play a role in age-related disc degeneration and matrix catabolism in humans and mouse models.
30811968	6	56	theme	old	835:837	arg1	mice					839:842	young and old mice	825:842	young and old mice	825:842	We first characterized the senescence status of discs in young and old mice.
30811968	7	57	theme	p16tdTom	897:904	arg1	reporter					906:913	a novel p16tdTom reporter	889:913	a novel p16tdTom reporter	889:913	Quantitative histology, gene expression and a novel p16tdTom reporter mice showed an increase in p16Ink4a, p21 and IL-6, with a decrease in Ki67 with aging.
30811968	1	58	theme	disc	186:189	arg1	contributor					220:230	an important contributor	207:230	an important contributor to chronic low back and neck pain	207:264	Intervertebral disc degeneration is an important contributor to chronic low back and neck pain.
30811968	1	58	theme	disc	186:189	arg1	degeneration					191:202	Intervertebral disc degeneration	171:202	Intervertebral disc degeneration	171:202	Intervertebral disc degeneration is an important contributor to chronic low back and neck pain.
30811968	8	59	with	spinal-phenotype	1030:1045	arg1	deletion					1085:1092	conditional deletion	1073:1092	conditional deletion of p16Ink4a in the disc driven by Acan-CreERT2 (cKO)	1073:1145	Accordingly, we studied the spinal-phenotype of 18-month-old mice with conditional deletion of p16Ink4a in the disc driven by Acan-CreERT2 (cKO).
30811968	4	60	from	phenotype	621:629	arg1	diseases					654:661	many age-associated diseases	634:661	many age-associated diseases	634:661	Clearance of p16Ink4a-positive senescent cells reduces the degenerative phenotype in many age-associated diseases.
30811968	9	61	theme	mice	1201:1204	arg1	discs					1164:1168	discs	1164:1168	discs of cKO and age-matched control mice	1164:1204	The analyses of discs of cKO and age-matched control mice showed little change in cell morphology and tissue architecture.
30811968	14	62	theme	SASP	1862:1865	arg1	IL-6					1883:1886	IL-6	1883:1886	IL-6	1883:1886	Interestingly, the cKO discs showed lower levels of SASP markers, IL-1β, IL-6, MCP1 and TGF-β1.
30811968	14	62	theme	SASP	1862:1865	arg1	TGF-β1					1898:1903	TGF-β1	1898:1903	TGF-β1	1898:1903	Interestingly, the cKO discs showed lower levels of SASP markers, IL-1β, IL-6, MCP1 and TGF-β1.
30811968	14	62	theme	SASP	1862:1865	arg1	IL-1β					1876:1880	IL-1β	1876:1880	IL-1β	1876:1880	Interestingly, the cKO discs showed lower levels of SASP markers, IL-1β, IL-6, MCP1 and TGF-β1.
30811968	14	62	theme	SASP	1862:1865	arg1	markers					1867:1873	SASP markers	1862:1873	SASP markers	1862:1873	Interestingly, the cKO discs showed lower levels of SASP markers, IL-1β, IL-6, MCP1 and TGF-β1.
30811968	14	62	theme	SASP	1862:1865	arg1	MCP1					1889:1892	MCP1	1889:1892	MCP1	1889:1892	Interestingly, the cKO discs showed lower levels of SASP markers, IL-1β, IL-6, MCP1 and TGF-β1.
30811968	12	63	theme	cKO	1598:1600	arg1	mice					1602:1605	The cKO mice	1594:1605	The cKO mice	1594:1605	The cKO mice maintained expression of NP-cell phenotypic markers CA3, Krt19 and GLUT-1.
30811968	6	64	theme	discs	816:820	arg1	status					806:811	the senescence status	791:811	the senescence status of discs in young and old mice	791:842	We first characterized the senescence status of discs in young and old mice.
30811968	7	65	theme	expression	874:883	arg1	mice					915:918	Quantitative histology, gene expression and a novel p16tdTom reporter mice	845:918	Quantitative histology, gene expression and a novel p16tdTom reporter mice	845:918	Quantitative histology, gene expression and a novel p16tdTom reporter mice showed an increase in p16Ink4a, p21 and IL-6, with a decrease in Ki67 with aging.
30811968	1	66	theme	important	210:218	arg1	contributor					220:230	an important contributor	207:230	an important contributor to chronic low back and neck pain	207:264	Intervertebral disc degeneration is an important contributor to chronic low back and neck pain.
30811968	1	66	theme	important	210:218	arg1	degeneration					191:202	Intervertebral disc degeneration	171:202	Intervertebral disc degeneration	171:202	Intervertebral disc degeneration is an important contributor to chronic low back and neck pain.
30811968	6	67	theme	senescence	795:804	arg1	status					806:811	the senescence status	791:811	the senescence status of discs in young and old mice	791:842	We first characterized the senescence status of discs in young and old mice.
30811968	11	68	from	changes	1548:1554	arg1	numbers					1572:1578	numbers	1572:1578	numbers	1572:1578	While cKO discs exhibited a small decrease in TUNEL positive cells, lineage tracing experiments using ZsGreen reporter indicated that the overall changes in cell fate or numbers were minimal.
30811968	11	68	from	changes	1548:1554	arg1	fate					1564:1567	cell fate	1559:1567	cell fate	1559:1567	While cKO discs exhibited a small decrease in TUNEL positive cells, lineage tracing experiments using ZsGreen reporter indicated that the overall changes in cell fate or numbers were minimal.
30811968	8	69	from	deletion	1085:1092	arg1	disc					1113:1116	the disc	1109:1116	the disc driven by Acan-CreERT2 (cKO)	1109:1145	Accordingly, we studied the spinal-phenotype of 18-month-old mice with conditional deletion of p16Ink4a in the disc driven by Acan-CreERT2 (cKO).
30811968	0	70	theme	senescence	159:168	arg1	onset					150:154	onset	150:154	onset of senescence	150:168	p16Ink4a deletion in cells of the intervertebral disc affects their matrix homeostasis and senescence associated secretory phenotype without altering onset of senescence.
30811968	13	71	from	alterations	1750:1760	arg1	deposition					1798:1807	Lipofuscin deposition	1787:1807	Lipofuscin deposition	1787:1807	Moreover, in cKO discs, levels of p19Arf and RB were higher without alterations in Ki67, γH2AX, CDK4 and Lipofuscin deposition.
30811968	13	71	from	alterations	1750:1760	arg1	γH2AX					1771:1775	γH2AX	1771:1775	γH2AX	1771:1775	Moreover, in cKO discs, levels of p19Arf and RB were higher without alterations in Ki67, γH2AX, CDK4 and Lipofuscin deposition.
30811968	13	71	from	alterations	1750:1760	arg1	Ki67					1765:1768	Ki67	1765:1768	Ki67	1765:1768	Moreover, in cKO discs, levels of p19Arf and RB were higher without alterations in Ki67, γH2AX, CDK4 and Lipofuscin deposition.
30811968	13	71	from	alterations	1750:1760	arg1	CDK4					1778:1781	CDK4	1778:1781	CDK4	1778:1781	Moreover, in cKO discs, levels of p19Arf and RB were higher without alterations in Ki67, γH2AX, CDK4 and Lipofuscin deposition.
30811968	14	72	theme	cKO	1829:1831	arg1	discs					1833:1837	the cKO discs	1825:1837	the cKO discs	1825:1837	Interestingly, the cKO discs showed lower levels of SASP markers, IL-1β, IL-6, MCP1 and TGF-β1.
30811968	11	73	theme	tracing	1478:1484	arg1	experiments					1486:1496	lineage tracing experiments	1470:1496	lineage tracing experiments using ZsGreen reporter	1470:1519	While cKO discs exhibited a small decrease in TUNEL positive cells, lineage tracing experiments using ZsGreen reporter indicated that the overall changes in cell fate or numbers were minimal.
30811968	9	74	theme	little	1213:1218	arg1	change					1220:1225	little change	1213:1225	little change in cell morphology and tissue architecture	1213:1268	The analyses of discs of cKO and age-matched control mice showed little change in cell morphology and tissue architecture.
30811968	5	75	theme	disc	723:726	arg1	degeneration					728:739	intervertebral disc degeneration	708:739	intervertebral disc degeneration	708:739	Whether p16Ink4a plays a functional role in intervertebral disc degeneration and senescence is unknown.
30811968	9	76	theme	tissue	1250:1255	arg1	architecture					1257:1268	tissue architecture	1250:1268	tissue architecture	1250:1268	The analyses of discs of cKO and age-matched control mice showed little change in cell morphology and tissue architecture.
30811968	2	77	theme	many	276:279	arg1	factors					307:313	many environmental and genetic factors	276:313	many environmental and genetic factors	276:313	Although many environmental and genetic factors are known to contribute to disc degeneration, age is still the most significant risk factor.
30811968	10	78	from	changes	1294:1300	arg1	attributes					1316:1325	functional attributes	1305:1325	functional attributes of aggrecan	1305:1337	The cKO mice exhibited changes in functional attributes of aggrecan as well as in collagen composition of the intervertebral disc.
30811968	10	78	from	changes	1294:1300	arg1	composition					1362:1372	collagen composition	1353:1372	collagen composition of the intervertebral disc	1353:1399	The cKO mice exhibited changes in functional attributes of aggrecan as well as in collagen composition of the intervertebral disc.
30811968	3	79	theme	age-related	469:479	arg1	degeneration					486:497	age-related disc degeneration	469:497	age-related disc degeneration	469:497	Recent studies have shown that senescence may play a role in age-related disc degeneration and matrix catabolism in humans and mouse models.
30811968	15	80	theme	disc	2113:2116	arg1	cells					2118:2122	disc cells	2113:2122	disc cells	2113:2122	These results show that while, p16Ink4a is dispensable for induction and maintenance of senescence, conditional loss of p16Ink4a reduces apoptosis, limits the SASP phenotype and alters matrix homeostasis of disc cells.
30811968	0	81	from	deletion	9:16	arg1	cells					21:25	cells	21:25	cells of the intervertebral disc	21:52	p16Ink4a deletion in cells of the intervertebral disc affects their matrix homeostasis and senescence associated secretory phenotype without altering onset of senescence.
30811968	8	82	theme	18-month-old	1050:1061	arg1	mice					1063:1066	18-month-old mice	1050:1066	18-month-old mice	1050:1066	Accordingly, we studied the spinal-phenotype of 18-month-old mice with conditional deletion of p16Ink4a in the disc driven by Acan-CreERT2 (cKO).
30811968	15	83	theme	senescence	1994:2003	arg1	induction					1965:1973	induction	1965:1973	induction	1965:1973	These results show that while, p16Ink4a is dispensable for induction and maintenance of senescence, conditional loss of p16Ink4a reduces apoptosis, limits the SASP phenotype and alters matrix homeostasis of disc cells.
30811968	15	83	theme	senescence	1994:2003	arg1	maintenance					1979:1989	maintenance	1979:1989	maintenance	1979:1989	These results show that while, p16Ink4a is dispensable for induction and maintenance of senescence, conditional loss of p16Ink4a reduces apoptosis, limits the SASP phenotype and alters matrix homeostasis of disc cells.
30811968	11	84	theme	small	1430:1434	arg1	decrease					1436:1443	a small decrease	1428:1443	a small decrease in TUNEL positive cells	1428:1467	While cKO discs exhibited a small decrease in TUNEL positive cells, lineage tracing experiments using ZsGreen reporter indicated that the overall changes in cell fate or numbers were minimal.
30811968	0	85	theme	secretory	113:121	arg1	phenotype					123:131	associated secretory phenotype	102:131	associated secretory phenotype	102:131	p16Ink4a deletion in cells of the intervertebral disc affects their matrix homeostasis and senescence associated secretory phenotype without altering onset of senescence.
30811968	12	86	theme	markers	1651:1657	arg1	expression					1618:1627	expression	1618:1627	expression of NP-cell phenotypic markers CA3, Krt19 and GLUT-1	1618:1679	The cKO mice maintained expression of NP-cell phenotypic markers CA3, Krt19 and GLUT-1.
30811968	10	87	theme	cKO	1275:1277	arg1	mice					1279:1282	The cKO mice	1271:1282	The cKO mice	1271:1282	The cKO mice exhibited changes in functional attributes of aggrecan as well as in collagen composition of the intervertebral disc.
30811968	2	88	theme	risk	395:398	arg1	factor					400:405	the most significant risk factor	374:405	the most significant risk factor	374:405	Although many environmental and genetic factors are known to contribute to disc degeneration, age is still the most significant risk factor.
30811968	2	88	theme	risk	395:398	arg1	age					361:363	age	361:363	age	361:363	Although many environmental and genetic factors are known to contribute to disc degeneration, age is still the most significant risk factor.
30811968	0	89	dep	homeostasis	75:85	arg1	phenotype					123:131	associated secretory phenotype	102:131	associated secretory phenotype	102:131	p16Ink4a deletion in cells of the intervertebral disc affects their matrix homeostasis and senescence associated secretory phenotype without altering onset of senescence.
30811968	11	90	theme	positive	1454:1461	arg1	cells					1463:1467	TUNEL positive cells	1448:1467	TUNEL positive cells	1448:1467	While cKO discs exhibited a small decrease in TUNEL positive cells, lineage tracing experiments using ZsGreen reporter indicated that the overall changes in cell fate or numbers were minimal.
30811968	7	91	theme	Quantitative	845:856	arg1	histology					858:866	Quantitative histology	845:866	Quantitative histology	845:866	Quantitative histology, gene expression and a novel p16tdTom reporter mice showed an increase in p16Ink4a, p21 and IL-6, with a decrease in Ki67 with aging.
30811968	8	92	theme	p16Ink4a	1097:1104	arg1	deletion					1085:1092	conditional deletion	1073:1092	conditional deletion of p16Ink4a in the disc driven by Acan-CreERT2 (cKO)	1073:1145	Accordingly, we studied the spinal-phenotype of 18-month-old mice with conditional deletion of p16Ink4a in the disc driven by Acan-CreERT2 (cKO).
30811968	4	93	theme	senescent	580:588	arg1	cells					590:594	p16Ink4a-positive senescent cells	562:594	p16Ink4a-positive senescent cells	562:594	Clearance of p16Ink4a-positive senescent cells reduces the degenerative phenotype in many age-associated diseases.
30811968	12	94	theme	NP-cell	1632:1638	arg1	CA3					1659:1661	CA3	1659:1661	CA3	1659:1661	The cKO mice maintained expression of NP-cell phenotypic markers CA3, Krt19 and GLUT-1.
30811968	12	94	theme	NP-cell	1632:1638	arg1	GLUT-1					1674:1679	GLUT-1	1674:1679	GLUT-1	1674:1679	The cKO mice maintained expression of NP-cell phenotypic markers CA3, Krt19 and GLUT-1.
30811968	12	94	theme	NP-cell	1632:1638	arg1	markers					1651:1657	NP-cell phenotypic markers CA3, Krt19 and GLUT-1	1632:1679	NP-cell phenotypic markers CA3, Krt19 and GLUT-1	1632:1679	The cKO mice maintained expression of NP-cell phenotypic markers CA3, Krt19 and GLUT-1.
30811968	12	94	theme	NP-cell	1632:1638	arg1	Krt19					1664:1668	Krt19	1664:1668	Krt19	1664:1668	The cKO mice maintained expression of NP-cell phenotypic markers CA3, Krt19 and GLUT-1.
30811968	0	95	theme	disc	49:52	arg1	cells					21:25	cells	21:25	cells of the intervertebral disc	21:52	p16Ink4a deletion in cells of the intervertebral disc affects their matrix homeostasis and senescence associated secretory phenotype without altering onset of senescence.
30811968	3	96	theme	Recent	408:413	arg1	studies					415:421	Recent studies	408:421	Recent studies	408:421	Recent studies have shown that senescence may play a role in age-related disc degeneration and matrix catabolism in humans and mouse models.
30811968	10	97	theme	aggrecan	1330:1337	arg1	attributes					1316:1325	functional attributes	1305:1325	functional attributes of aggrecan	1305:1337	The cKO mice exhibited changes in functional attributes of aggrecan as well as in collagen composition of the intervertebral disc.
30811968	11	98	theme	cell	1559:1562	arg1	fate					1564:1567	cell fate	1559:1567	cell fate	1559:1567	While cKO discs exhibited a small decrease in TUNEL positive cells, lineage tracing experiments using ZsGreen reporter indicated that the overall changes in cell fate or numbers were minimal.
30811968	3	99	theme	disc	481:484	arg1	degeneration					486:497	age-related disc degeneration	469:497	age-related disc degeneration	469:497	Recent studies have shown that senescence may play a role in age-related disc degeneration and matrix catabolism in humans and mouse models.
30811968	15	100	theme	conditional	2006:2016	arg1	loss					2018:2021	conditional loss	2006:2021	conditional loss of p16Ink4a	2006:2033	These results show that while, p16Ink4a is dispensable for induction and maintenance of senescence, conditional loss of p16Ink4a reduces apoptosis, limits the SASP phenotype and alters matrix homeostasis of disc cells.
30811968	4	101	theme	age-associated	639:652	arg1	diseases					654:661	many age-associated diseases	634:661	many age-associated diseases	634:661	Clearance of p16Ink4a-positive senescent cells reduces the degenerative phenotype in many age-associated diseases.
30811968	1	102	theme	neck	256:259	arg1	pain					261:264	neck pain	256:264	neck pain	256:264	Intervertebral disc degeneration is an important contributor to chronic low back and neck pain.
29981426	2	0	from	critical	542:549	arg1	response					558:565	the response	554:565	the response	554:565	Studies suggest that the effects of early-life immune activation may not occur until a secondary stressor is introduced, though the precise nature and timing of the stressor may be critical in the response.
29981426	10	1	theme	secondary	2651:2659	arg1	challenge					2661:2669	a secondary challenge	2649:2669	a secondary challenge	2649:2669	Collectively, these results provide novel evidence that some of the sex-specific behavioral consequences of an early-life immune challenge may not transpire until an individual is faced with a secondary challenge, and the context in which an individual is exposed can greatly influence the response.
29981426	5	2	theme	early-life	1156:1165	arg1	infection					1167:1175	an early-life infection	1153:1175	an early-life infection	1153:1175	In order to test the hypothesis that an early-life immune challenge makes individuals more vulnerable to the effects of antibiotics, we mimicked an early-life infection by injecting pups at postnatal day 3 and 5 with lipopolysaccharide (LPS; cell wall component of gram-negative bacteria) or saline, and subsequently exposed the same animals to antibiotic treatment (known to influence microbial community composition and behavior) or water in adulthood.
29981426	4	3	theme	immune	827:832	arg1	system					834:839	the immune system	823:839	the immune system	823:839	Here, we sought to determine whether early-life activation of the immune system had long-term consequences on how the gut microbiome responds to antibiotic treatment in adulthood and whether those changes influence adult same-sex social behavior.
29981426	5	4	dep	LPS	1245:1247	arg1	component					1260:1268	cell wall component	1250:1268	LPS; cell wall component of gram-negative bacteria	1245:1294	In order to test the hypothesis that an early-life immune challenge makes individuals more vulnerable to the effects of antibiotics, we mimicked an early-life infection by injecting pups at postnatal day 3 and 5 with lipopolysaccharide (LPS; cell wall component of gram-negative bacteria) or saline, and subsequently exposed the same animals to antibiotic treatment (known to influence microbial community composition and behavior) or water in adulthood.
29981426	8	5	theme	robust	2113:2118	arg1	changes					2120:2126	more robust changes	2108:2126	more robust changes in their behavioral response	2108:2155	Interestingly, LPS-treated males exhibited more robust changes in their behavioral response following adult antibiotic treatment, including decreased investigation and increased grooming, suggestive of changes in anxiety-like behaviors.
29981426	1	6	from	interest	301:308	arg1	study					317:321	the study	313:321	the study of developmental origins of behavior	313:358	Although it is well-established that the immune system plays an important role in the development of physiology and behavior, the gut microbiome has recently become of interest in the study of developmental origins of behavior.
29981426	1	7	theme	gut	263:265	arg1	microbiome					267:276	the gut microbiome	259:276	the gut microbiome	259:276	Although it is well-established that the immune system plays an important role in the development of physiology and behavior, the gut microbiome has recently become of interest in the study of developmental origins of behavior.
29981426	8	8	from	changes	2267:2273	arg1	behaviors					2291:2299	anxiety-like behaviors	2278:2299	anxiety-like behaviors	2278:2299	Interestingly, LPS-treated males exhibited more robust changes in their behavioral response following adult antibiotic treatment, including decreased investigation and increased grooming, suggestive of changes in anxiety-like behaviors.
29981426	5	9	from	day	1208:1210	arg1	pups					1190:1193	pups	1190:1193	pups at postnatal day 3 and 5	1190:1218	In order to test the hypothesis that an early-life immune challenge makes individuals more vulnerable to the effects of antibiotics, we mimicked an early-life infection by injecting pups at postnatal day 3 and 5 with lipopolysaccharide (LPS; cell wall component of gram-negative bacteria) or saline, and subsequently exposed the same animals to antibiotic treatment (known to influence microbial community composition and behavior) or water in adulthood.
29981426	5	10	theme	early-life	1048:1057	arg1	challenge					1066:1074	an early-life immune challenge	1045:1074	an early-life immune challenge	1045:1074	In order to test the hypothesis that an early-life immune challenge makes individuals more vulnerable to the effects of antibiotics, we mimicked an early-life infection by injecting pups at postnatal day 3 and 5 with lipopolysaccharide (LPS; cell wall component of gram-negative bacteria) or saline, and subsequently exposed the same animals to antibiotic treatment (known to influence microbial community composition and behavior) or water in adulthood.
29981426	7	11	theme	gut	1927:1929	arg1	communities					1941:1951	female and male gut microbial communities	1911:1951	female and male gut microbial communities	1911:1951	LPS-treated females exhibited impaired reproductive physiology and function in adulthood (e.g., smaller ovaries and abnormal estrous cycles), and female and male gut microbial communities were strongly affected by antibiotic treatment in adulthood, but only slightly affected by postnatal LPS alone.
29981426	6	12	from	antibiotics	1752:1762	arg1	recovery					1738:1745	recovery	1738:1745	recovery from antibiotics	1738:1762	We tracked physiology across development, and paired males and females with a novel individual of the same age and sex in adulthood to score same-sex behavior (e.g., aggression, investigation, grooming) before antibiotic treatment, immediately following treatment, and after recovery from antibiotics.
29981426	6	13	dep	tracked	1466:1472	arg1	grooming					1656:1663	grooming	1656:1663	grooming	1656:1663	We tracked physiology across development, and paired males and females with a novel individual of the same age and sex in adulthood to score same-sex behavior (e.g., aggression, investigation, grooming) before antibiotic treatment, immediately following treatment, and after recovery from antibiotics.
29981426	6	13	dep	tracked	1466:1472	arg1	investigation					1641:1653	investigation	1641:1653	investigation	1641:1653	We tracked physiology across development, and paired males and females with a novel individual of the same age and sex in adulthood to score same-sex behavior (e.g., aggression, investigation, grooming) before antibiotic treatment, immediately following treatment, and after recovery from antibiotics.
29981426	6	13	dep	tracked	1466:1472	arg1	aggression					1629:1638	aggression	1629:1638	aggression	1629:1638	We tracked physiology across development, and paired males and females with a novel individual of the same age and sex in adulthood to score same-sex behavior (e.g., aggression, investigation, grooming) before antibiotic treatment, immediately following treatment, and after recovery from antibiotics.
29981426	8	14	theme	behavioral	2137:2146	arg1	response					2148:2155	their behavioral response	2131:2155	their behavioral response	2131:2155	Interestingly, LPS-treated males exhibited more robust changes in their behavioral response following adult antibiotic treatment, including decreased investigation and increased grooming, suggestive of changes in anxiety-like behaviors.
29981426	7	15	dep	exhibited	1785:1793	arg1	ovaries					1869:1875	smaller ovaries	1861:1875	smaller ovaries	1861:1875	LPS-treated females exhibited impaired reproductive physiology and function in adulthood (e.g., smaller ovaries and abnormal estrous cycles), and female and male gut microbial communities were strongly affected by antibiotic treatment in adulthood, but only slightly affected by postnatal LPS alone.
29981426	7	15	dep	exhibited	1785:1793	arg1	cycles					1898:1903	abnormal estrous cycles	1881:1903	abnormal estrous cycles	1881:1903	LPS-treated females exhibited impaired reproductive physiology and function in adulthood (e.g., smaller ovaries and abnormal estrous cycles), and female and male gut microbial communities were strongly affected by antibiotic treatment in adulthood, but only slightly affected by postnatal LPS alone.
29981426	3	16	from	perturbations	679:691	arg1	life					726:729	life	726:729	life	726:729	Further, recent work suggests that the microbiome and the immune system develop in parallel, and therefore any perturbations to one of these systems early in life will likely affect the other.
29981426	3	17	theme	systems	709:715	arg1	systems					709:715	these systems	703:715	these systems	703:715	Further, recent work suggests that the microbiome and the immune system develop in parallel, and therefore any perturbations to one of these systems early in life will likely affect the other.
29981426	3	17	theme	systems	709:715	arg1	one					696:698	one	696:698	one	696:698	Further, recent work suggests that the microbiome and the immune system develop in parallel, and therefore any perturbations to one of these systems early in life will likely affect the other.
29981426	5	18	theme	antibiotic	1353:1362	arg1	treatment					1364:1372	antibiotic treatment	1353:1372	antibiotic treatment (known to influence microbial community composition and behavior)	1353:1438	In order to test the hypothesis that an early-life immune challenge makes individuals more vulnerable to the effects of antibiotics, we mimicked an early-life infection by injecting pups at postnatal day 3 and 5 with lipopolysaccharide (LPS; cell wall component of gram-negative bacteria) or saline, and subsequently exposed the same animals to antibiotic treatment (known to influence microbial community composition and behavior) or water in adulthood.
29981426	4	19	theme	gut	879:881	arg1	microbiome					883:892	the gut microbiome	875:892	the gut microbiome	875:892	Here, we sought to determine whether early-life activation of the immune system had long-term consequences on how the gut microbiome responds to antibiotic treatment in adulthood and whether those changes influence adult same-sex social behavior.
29981426	0	20	from	alterations	85:95	arg1	adulthood					122:130	adulthood	122:130	adulthood	122:130	Early-life sickness may predispose Siberian hamsters to behavioral changes following alterations of the gut microbiome in adulthood.
29981426	8	21	theme	antibiotic	2173:2182	arg1	treatment					2184:2192	adult antibiotic treatment	2167:2192	adult antibiotic treatment	2167:2192	Interestingly, LPS-treated males exhibited more robust changes in their behavioral response following adult antibiotic treatment, including decreased investigation and increased grooming, suggestive of changes in anxiety-like behaviors.
29981426	5	22	from	water	1443:1447	arg1	adulthood					1452:1460	adulthood	1452:1460	adulthood	1452:1460	In order to test the hypothesis that an early-life immune challenge makes individuals more vulnerable to the effects of antibiotics, we mimicked an early-life infection by injecting pups at postnatal day 3 and 5 with lipopolysaccharide (LPS; cell wall component of gram-negative bacteria) or saline, and subsequently exposed the same animals to antibiotic treatment (known to influence microbial community composition and behavior) or water in adulthood.
29981426	6	23	theme	same-sex	1604:1611	arg1	behavior					1613:1620	score same-sex behavior	1598:1620	score same-sex behavior	1598:1620	We tracked physiology across development, and paired males and females with a novel individual of the same age and sex in adulthood to score same-sex behavior (e.g., aggression, investigation, grooming) before antibiotic treatment, immediately following treatment, and after recovery from antibiotics.
29981426	8	24	theme	decreased	2205:2213	arg1	investigation					2215:2227	decreased investigation	2205:2227	decreased investigation	2205:2227	Interestingly, LPS-treated males exhibited more robust changes in their behavioral response following adult antibiotic treatment, including decreased investigation and increased grooming, suggestive of changes in anxiety-like behaviors.
29981426	9	25	theme	immune	2426:2431	arg1	challenge					2433:2441	an immune challenge	2423:2441	an immune challenge early in life	2423:2455	These data suggest that males may be more vulnerable than females to behavioral abnormalities after being predisposed to an immune challenge early in life.
29981426	1	26	theme	important	197:205	arg1	role					207:210	an important role	194:210	an important role	194:210	Although it is well-established that the immune system plays an important role in the development of physiology and behavior, the gut microbiome has recently become of interest in the study of developmental origins of behavior.
29981426	10	27	theme	behavioral	2539:2548	arg1	consequences					2550:2561	the sex-specific behavioral consequences	2522:2561	the sex-specific behavioral consequences of an early-life immune challenge	2522:2595	Collectively, these results provide novel evidence that some of the sex-specific behavioral consequences of an early-life immune challenge may not transpire until an individual is faced with a secondary challenge, and the context in which an individual is exposed can greatly influence the response.
29981426	9	28	theme	early	2443:2447	arg1	challenge					2433:2441	an immune challenge	2423:2441	an immune challenge early in life	2423:2455	These data suggest that males may be more vulnerable than females to behavioral abnormalities after being predisposed to an immune challenge early in life.
29981426	1	29	theme	developmental	326:338	arg1	origins					340:346	developmental origins	326:346	developmental origins of behavior	326:358	Although it is well-established that the immune system plays an important role in the development of physiology and behavior, the gut microbiome has recently become of interest in the study of developmental origins of behavior.
29981426	6	30	theme	sex	1578:1580	arg1	individual					1547:1556	a novel individual	1539:1556	a novel individual of the same age and sex in adulthood to score same-sex behavior	1539:1620	We tracked physiology across development, and paired males and females with a novel individual of the same age and sex in adulthood to score same-sex behavior (e.g., aggression, investigation, grooming) before antibiotic treatment, immediately following treatment, and after recovery from antibiotics.
29981426	10	31	theme	early-life	2569:2578	arg1	challenge					2587:2595	an early-life immune challenge	2566:2595	an early-life immune challenge	2566:2595	Collectively, these results provide novel evidence that some of the sex-specific behavioral consequences of an early-life immune challenge may not transpire until an individual is faced with a secondary challenge, and the context in which an individual is exposed can greatly influence the response.
29981426	7	32	theme	abnormal	1881:1888	arg1	cycles					1898:1903	abnormal estrous cycles	1881:1903	abnormal estrous cycles	1881:1903	LPS-treated females exhibited impaired reproductive physiology and function in adulthood (e.g., smaller ovaries and abnormal estrous cycles), and female and male gut microbial communities were strongly affected by antibiotic treatment in adulthood, but only slightly affected by postnatal LPS alone.
29981426	6	33	theme	age	1570:1572	arg1	individual					1547:1556	a novel individual	1539:1556	a novel individual of the same age and sex in adulthood to score same-sex behavior	1539:1620	We tracked physiology across development, and paired males and females with a novel individual of the same age and sex in adulthood to score same-sex behavior (e.g., aggression, investigation, grooming) before antibiotic treatment, immediately following treatment, and after recovery from antibiotics.
29981426	4	34	theme	antibiotic	906:915	arg1	treatment					917:925	antibiotic treatment	906:925	antibiotic treatment	906:925	Here, we sought to determine whether early-life activation of the immune system had long-term consequences on how the gut microbiome responds to antibiotic treatment in adulthood and whether those changes influence adult same-sex social behavior.
29981426	5	35	theme	microbial	1394:1402	arg1	composition					1414:1424	microbial community composition	1394:1424	microbial community composition	1394:1424	In order to test the hypothesis that an early-life immune challenge makes individuals more vulnerable to the effects of antibiotics, we mimicked an early-life infection by injecting pups at postnatal day 3 and 5 with lipopolysaccharide (LPS; cell wall component of gram-negative bacteria) or saline, and subsequently exposed the same animals to antibiotic treatment (known to influence microbial community composition and behavior) or water in adulthood.
29981426	5	36	theme	gram-negative	1273:1285	arg1	bacteria					1287:1294	gram-negative bacteria	1273:1294	gram-negative bacteria	1273:1294	In order to test the hypothesis that an early-life immune challenge makes individuals more vulnerable to the effects of antibiotics, we mimicked an early-life infection by injecting pups at postnatal day 3 and 5 with lipopolysaccharide (LPS; cell wall component of gram-negative bacteria) or saline, and subsequently exposed the same animals to antibiotic treatment (known to influence microbial community composition and behavior) or water in adulthood.
29981426	0	37	theme	microbiome	108:117	arg1	alterations					85:95	alterations	85:95	alterations of the gut microbiome in adulthood	85:130	Early-life sickness may predispose Siberian hamsters to behavioral changes following alterations of the gut microbiome in adulthood.
29981426	5	38	theme	immune	1059:1064	arg1	challenge					1066:1074	an early-life immune challenge	1045:1074	an early-life immune challenge	1045:1074	In order to test the hypothesis that an early-life immune challenge makes individuals more vulnerable to the effects of antibiotics, we mimicked an early-life infection by injecting pups at postnatal day 3 and 5 with lipopolysaccharide (LPS; cell wall component of gram-negative bacteria) or saline, and subsequently exposed the same animals to antibiotic treatment (known to influence microbial community composition and behavior) or water in adulthood.
29981426	4	39	theme	same-sex	982:989	arg1	behavior					998:1005	adult same-sex social behavior	976:1005	adult same-sex social behavior	976:1005	Here, we sought to determine whether early-life activation of the immune system had long-term consequences on how the gut microbiome responds to antibiotic treatment in adulthood and whether those changes influence adult same-sex social behavior.
29981426	0	40	theme	Early-life	0:9	arg1	sickness					11:18	Early-life sickness	0:18	Early-life sickness	0:18	Early-life sickness may predispose Siberian hamsters to behavioral changes following alterations of the gut microbiome in adulthood.
29981426	5	41	theme	antibiotics	1128:1138	arg1	effects					1117:1123	the effects	1113:1123	the effects of antibiotics	1113:1138	In order to test the hypothesis that an early-life immune challenge makes individuals more vulnerable to the effects of antibiotics, we mimicked an early-life infection by injecting pups at postnatal day 3 and 5 with lipopolysaccharide (LPS; cell wall component of gram-negative bacteria) or saline, and subsequently exposed the same animals to antibiotic treatment (known to influence microbial community composition and behavior) or water in adulthood.
29981426	6	42	dep	age	1570:1572	arg1	the					1561:1563	the	1561:1563	the	1561:1563	We tracked physiology across development, and paired males and females with a novel individual of the same age and sex in adulthood to score same-sex behavior (e.g., aggression, investigation, grooming) before antibiotic treatment, immediately following treatment, and after recovery from antibiotics.
29981426	2	43	from	response	558:565	arg1	critical					542:549	critical	542:549	critical	542:549	Studies suggest that the effects of early-life immune activation may not occur until a secondary stressor is introduced, though the precise nature and timing of the stressor may be critical in the response.
29981426	10	44	theme	challenge	2587:2595	arg1	consequences					2550:2561	the sex-specific behavioral consequences	2522:2561	the sex-specific behavioral consequences of an early-life immune challenge	2522:2595	Collectively, these results provide novel evidence that some of the sex-specific behavioral consequences of an early-life immune challenge may not transpire until an individual is faced with a secondary challenge, and the context in which an individual is exposed can greatly influence the response.
29981426	5	45	theme	cell	1250:1253	arg1	component					1260:1268	cell wall component	1250:1268	LPS; cell wall component of gram-negative bacteria	1245:1294	In order to test the hypothesis that an early-life immune challenge makes individuals more vulnerable to the effects of antibiotics, we mimicked an early-life infection by injecting pups at postnatal day 3 and 5 with lipopolysaccharide (LPS; cell wall component of gram-negative bacteria) or saline, and subsequently exposed the same animals to antibiotic treatment (known to influence microbial community composition and behavior) or water in adulthood.
29981426	2	46	theme	activation	415:424	arg1	effects					386:392	the effects	382:392	the effects of early-life immune activation	382:424	Studies suggest that the effects of early-life immune activation may not occur until a secondary stressor is introduced, though the precise nature and timing of the stressor may be critical in the response.
29981426	6	47	from	adulthood	1585:1593	arg1	individual					1547:1556	a novel individual	1539:1556	a novel individual of the same age and sex in adulthood to score same-sex behavior	1539:1620	We tracked physiology across development, and paired males and females with a novel individual of the same age and sex in adulthood to score same-sex behavior (e.g., aggression, investigation, grooming) before antibiotic treatment, immediately following treatment, and after recovery from antibiotics.
29981426	2	48	theme	early-life	397:406	arg1	activation					415:424	early-life immune activation	397:424	early-life immune activation	397:424	Studies suggest that the effects of early-life immune activation may not occur until a secondary stressor is introduced, though the precise nature and timing of the stressor may be critical in the response.
29981426	0	49	theme	Siberian	35:42	arg1	hamsters					44:51	Siberian hamsters	35:51	Siberian hamsters	35:51	Early-life sickness may predispose Siberian hamsters to behavioral changes following alterations of the gut microbiome in adulthood.
29981426	2	50	theme	secondary	448:456	arg1	stressor					458:465	a secondary stressor	446:465	a secondary stressor	446:465	Studies suggest that the effects of early-life immune activation may not occur until a secondary stressor is introduced, though the precise nature and timing of the stressor may be critical in the response.
29981426	6	51	dep	treatment	1717:1725	arg1	following					1707:1715	following	1707:1715	following	1707:1715	We tracked physiology across development, and paired males and females with a novel individual of the same age and sex in adulthood to score same-sex behavior (e.g., aggression, investigation, grooming) before antibiotic treatment, immediately following treatment, and after recovery from antibiotics.
29981426	5	52	from	treatment	1364:1372	arg1	adulthood					1452:1460	adulthood	1452:1460	adulthood	1452:1460	In order to test the hypothesis that an early-life immune challenge makes individuals more vulnerable to the effects of antibiotics, we mimicked an early-life infection by injecting pups at postnatal day 3 and 5 with lipopolysaccharide (LPS; cell wall component of gram-negative bacteria) or saline, and subsequently exposed the same animals to antibiotic treatment (known to influence microbial community composition and behavior) or water in adulthood.
29981426	6	53	theme	antibiotic	1673:1682	arg1	treatment					1684:1692	antibiotic treatment	1673:1692	antibiotic treatment	1673:1692	We tracked physiology across development, and paired males and females with a novel individual of the same age and sex in adulthood to score same-sex behavior (e.g., aggression, investigation, grooming) before antibiotic treatment, immediately following treatment, and after recovery from antibiotics.
29981426	5	54	theme	same	1337:1340	arg1	animals					1342:1348	the same animals	1333:1348	the same animals	1333:1348	In order to test the hypothesis that an early-life immune challenge makes individuals more vulnerable to the effects of antibiotics, we mimicked an early-life infection by injecting pups at postnatal day 3 and 5 with lipopolysaccharide (LPS; cell wall component of gram-negative bacteria) or saline, and subsequently exposed the same animals to antibiotic treatment (known to influence microbial community composition and behavior) or water in adulthood.
29981426	7	55	theme	reproductive	1804:1815	arg1	physiology					1817:1826	impaired reproductive physiology	1795:1826	impaired reproductive physiology	1795:1826	LPS-treated females exhibited impaired reproductive physiology and function in adulthood (e.g., smaller ovaries and abnormal estrous cycles), and female and male gut microbial communities were strongly affected by antibiotic treatment in adulthood, but only slightly affected by postnatal LPS alone.
29981426	7	56	from	treatment	1990:1998	arg1	adulthood					2003:2011	adulthood	2003:2011	adulthood	2003:2011	LPS-treated females exhibited impaired reproductive physiology and function in adulthood (e.g., smaller ovaries and abnormal estrous cycles), and female and male gut microbial communities were strongly affected by antibiotic treatment in adulthood, but only slightly affected by postnatal LPS alone.
29981426	1	57	theme	physiology	234:243	arg1	development					219:229	the development	215:229	the development of physiology and behavior	215:256	Although it is well-established that the immune system plays an important role in the development of physiology and behavior, the gut microbiome has recently become of interest in the study of developmental origins of behavior.
29981426	1	58	theme	behavior	249:256	arg1	development					219:229	the development	215:229	the development of physiology and behavior	215:256	Although it is well-established that the immune system plays an important role in the development of physiology and behavior, the gut microbiome has recently become of interest in the study of developmental origins of behavior.
29981426	7	59	theme	antibiotic	1979:1988	arg1	treatment					1990:1998	antibiotic treatment	1979:1998	antibiotic treatment in adulthood	1979:2011	LPS-treated females exhibited impaired reproductive physiology and function in adulthood (e.g., smaller ovaries and abnormal estrous cycles), and female and male gut microbial communities were strongly affected by antibiotic treatment in adulthood, but only slightly affected by postnatal LPS alone.
29981426	4	60	theme	early-life	798:807	arg1	activation					809:818	early-life activation	798:818	early-life activation of the immune system	798:839	Here, we sought to determine whether early-life activation of the immune system had long-term consequences on how the gut microbiome responds to antibiotic treatment in adulthood and whether those changes influence adult same-sex social behavior.
29981426	5	61	from	5	1218:1218	arg1	pups					1190:1193	pups	1190:1193	pups at postnatal day 3 and 5	1190:1218	In order to test the hypothesis that an early-life immune challenge makes individuals more vulnerable to the effects of antibiotics, we mimicked an early-life infection by injecting pups at postnatal day 3 and 5 with lipopolysaccharide (LPS; cell wall component of gram-negative bacteria) or saline, and subsequently exposed the same animals to antibiotic treatment (known to influence microbial community composition and behavior) or water in adulthood.
29981426	9	62	from	early	2443:2447	arg1	life					2452:2455	life	2452:2455	life	2452:2455	These data suggest that males may be more vulnerable than females to behavioral abnormalities after being predisposed to an immune challenge early in life.
29981426	1	63	theme	immune	174:179	arg1	system					181:186	the immune system	170:186	the immune system	170:186	Although it is well-established that the immune system plays an important role in the development of physiology and behavior, the gut microbiome has recently become of interest in the study of developmental origins of behavior.
29981426	6	64	from	sex	1578:1580	arg1	adulthood					1585:1593	adulthood	1585:1593	adulthood	1585:1593	We tracked physiology across development, and paired males and females with a novel individual of the same age and sex in adulthood to score same-sex behavior (e.g., aggression, investigation, grooming) before antibiotic treatment, immediately following treatment, and after recovery from antibiotics.
29981426	7	65	theme	microbial	1931:1939	arg1	communities					1941:1951	female and male gut microbial communities	1911:1951	female and male gut microbial communities	1911:1951	LPS-treated females exhibited impaired reproductive physiology and function in adulthood (e.g., smaller ovaries and abnormal estrous cycles), and female and male gut microbial communities were strongly affected by antibiotic treatment in adulthood, but only slightly affected by postnatal LPS alone.
29981426	8	66	theme	changes	2267:2273	arg1	suggestive					2253:2262	suggestive	2253:2262	suggestive	2253:2262	Interestingly, LPS-treated males exhibited more robust changes in their behavioral response following adult antibiotic treatment, including decreased investigation and increased grooming, suggestive of changes in anxiety-like behaviors.
29981426	9	67	theme	behavioral	2371:2380	arg1	abnormalities					2382:2394	behavioral abnormalities	2371:2394	behavioral abnormalities	2371:2394	These data suggest that males may be more vulnerable than females to behavioral abnormalities after being predisposed to an immune challenge early in life.
29981426	6	68	from	individual	1547:1556	arg1	adulthood					1585:1593	adulthood	1585:1593	adulthood	1585:1593	We tracked physiology across development, and paired males and females with a novel individual of the same age and sex in adulthood to score same-sex behavior (e.g., aggression, investigation, grooming) before antibiotic treatment, immediately following treatment, and after recovery from antibiotics.
29981426	7	69	theme	male	1922:1925	arg1	communities					1941:1951	female and male gut microbial communities	1911:1951	female and male gut microbial communities	1911:1951	LPS-treated females exhibited impaired reproductive physiology and function in adulthood (e.g., smaller ovaries and abnormal estrous cycles), and female and male gut microbial communities were strongly affected by antibiotic treatment in adulthood, but only slightly affected by postnatal LPS alone.
29981426	2	70	theme	stressor	526:533	arg1	nature					501:506	precise nature	493:506	precise nature	493:506	Studies suggest that the effects of early-life immune activation may not occur until a secondary stressor is introduced, though the precise nature and timing of the stressor may be critical in the response.
29981426	2	70	theme	stressor	526:533	arg1	timing					512:517	timing	512:517	timing	512:517	Studies suggest that the effects of early-life immune activation may not occur until a secondary stressor is introduced, though the precise nature and timing of the stressor may be critical in the response.
29981426	6	71	with	development	1492:1502	arg1	individual					1547:1556	a novel individual	1539:1556	a novel individual of the same age and sex in adulthood to score same-sex behavior	1539:1620	We tracked physiology across development, and paired males and females with a novel individual of the same age and sex in adulthood to score same-sex behavior (e.g., aggression, investigation, grooming) before antibiotic treatment, immediately following treatment, and after recovery from antibiotics.
29981426	7	72	theme	female	1911:1916	arg1	communities					1941:1951	female and male gut microbial communities	1911:1951	female and male gut microbial communities	1911:1951	LPS-treated females exhibited impaired reproductive physiology and function in adulthood (e.g., smaller ovaries and abnormal estrous cycles), and female and male gut microbial communities were strongly affected by antibiotic treatment in adulthood, but only slightly affected by postnatal LPS alone.
29981426	8	73	theme	adult	2167:2171	arg1	treatment					2184:2192	adult antibiotic treatment	2167:2192	adult antibiotic treatment	2167:2192	Interestingly, LPS-treated males exhibited more robust changes in their behavioral response following adult antibiotic treatment, including decreased investigation and increased grooming, suggestive of changes in anxiety-like behaviors.
29981426	5	74	theme	vulnerable	1099:1108	arg1	individuals					1082:1092	individuals	1082:1092	individuals more vulnerable to the effects of antibiotics	1082:1138	In order to test the hypothesis that an early-life immune challenge makes individuals more vulnerable to the effects of antibiotics, we mimicked an early-life infection by injecting pups at postnatal day 3 and 5 with lipopolysaccharide (LPS; cell wall component of gram-negative bacteria) or saline, and subsequently exposed the same animals to antibiotic treatment (known to influence microbial community composition and behavior) or water in adulthood.
29981426	4	75	theme	system	834:839	arg1	activation					809:818	early-life activation	798:818	early-life activation of the immune system	798:839	Here, we sought to determine whether early-life activation of the immune system had long-term consequences on how the gut microbiome responds to antibiotic treatment in adulthood and whether those changes influence adult same-sex social behavior.
29981426	6	76	theme	score	1598:1602	arg1	behavior					1613:1620	score same-sex behavior	1598:1620	score same-sex behavior	1598:1620	We tracked physiology across development, and paired males and females with a novel individual of the same age and sex in adulthood to score same-sex behavior (e.g., aggression, investigation, grooming) before antibiotic treatment, immediately following treatment, and after recovery from antibiotics.
29981426	7	77	theme	postnatal	2044:2052	arg1	LPS					2054:2056	postnatal LPS	2044:2056	postnatal LPS alone	2044:2062	LPS-treated females exhibited impaired reproductive physiology and function in adulthood (e.g., smaller ovaries and abnormal estrous cycles), and female and male gut microbial communities were strongly affected by antibiotic treatment in adulthood, but only slightly affected by postnatal LPS alone.
29981426	8	78	from	changes	2120:2126	arg1	response					2148:2155	their behavioral response	2131:2155	their behavioral response	2131:2155	Interestingly, LPS-treated males exhibited more robust changes in their behavioral response following adult antibiotic treatment, including decreased investigation and increased grooming, suggestive of changes in anxiety-like behaviors.
29981426	6	79	dep	aggression	1629:1638	arg1	e.g.					1623:1626	e.g.	1623:1626	e.g.	1623:1626	We tracked physiology across development, and paired males and females with a novel individual of the same age and sex in adulthood to score same-sex behavior (e.g., aggression, investigation, grooming) before antibiotic treatment, immediately following treatment, and after recovery from antibiotics.
29981426	4	80	theme	long-term	845:853	arg1	consequences					855:866	long-term consequences	845:866	long-term consequences	845:866	Here, we sought to determine whether early-life activation of the immune system had long-term consequences on how the gut microbiome responds to antibiotic treatment in adulthood and whether those changes influence adult same-sex social behavior.
29981426	10	81	theme	novel	2494:2498	arg1	evidence					2500:2507	novel evidence that some of the sex-specific behavioral consequences of an early-life immune challenge may not transpire until an individual is faced with a secondary challenge, and the context in which an individual is exposed can greatly influence the response	2494:2755	novel evidence that some of the sex-specific behavioral consequences of an early-life immune challenge may not transpire until an individual is faced with a secondary challenge, and the context in which an individual is exposed can greatly influence the response	2494:2755	Collectively, these results provide novel evidence that some of the sex-specific behavioral consequences of an early-life immune challenge may not transpire until an individual is faced with a secondary challenge, and the context in which an individual is exposed can greatly influence the response.
29981426	6	82	with	females	1526:1532	arg1	individual					1547:1556	a novel individual	1539:1556	a novel individual of the same age and sex in adulthood to score same-sex behavior	1539:1620	We tracked physiology across development, and paired males and females with a novel individual of the same age and sex in adulthood to score same-sex behavior (e.g., aggression, investigation, grooming) before antibiotic treatment, immediately following treatment, and after recovery from antibiotics.
29981426	3	83	theme	recent	577:582	arg1	work					584:587	recent work	577:587	recent work	577:587	Further, recent work suggests that the microbiome and the immune system develop in parallel, and therefore any perturbations to one of these systems early in life will likely affect the other.
29981426	6	84	theme	paired	1509:1514	arg1	males					1516:1520	paired males	1509:1520	paired males	1509:1520	We tracked physiology across development, and paired males and females with a novel individual of the same age and sex in adulthood to score same-sex behavior (e.g., aggression, investigation, grooming) before antibiotic treatment, immediately following treatment, and after recovery from antibiotics.
29981426	10	85	theme	sex-specific	2526:2537	arg1	consequences					2550:2561	the sex-specific behavioral consequences	2522:2561	the sex-specific behavioral consequences of an early-life immune challenge	2522:2595	Collectively, these results provide novel evidence that some of the sex-specific behavioral consequences of an early-life immune challenge may not transpire until an individual is faced with a secondary challenge, and the context in which an individual is exposed can greatly influence the response.
29981426	7	86	theme	estrous	1890:1896	arg1	cycles					1898:1903	abnormal estrous cycles	1881:1903	abnormal estrous cycles	1881:1903	LPS-treated females exhibited impaired reproductive physiology and function in adulthood (e.g., smaller ovaries and abnormal estrous cycles), and female and male gut microbial communities were strongly affected by antibiotic treatment in adulthood, but only slightly affected by postnatal LPS alone.
29981426	8	87	theme	anxiety-like	2278:2289	arg1	behaviors					2291:2299	anxiety-like behaviors	2278:2299	anxiety-like behaviors	2278:2299	Interestingly, LPS-treated males exhibited more robust changes in their behavioral response following adult antibiotic treatment, including decreased investigation and increased grooming, suggestive of changes in anxiety-like behaviors.
29981426	0	88	theme	gut	104:106	arg1	microbiome					108:117	the gut microbiome	100:117	the gut microbiome	100:117	Early-life sickness may predispose Siberian hamsters to behavioral changes following alterations of the gut microbiome in adulthood.
29981426	1	89	theme	origins	340:346	arg1	study					317:321	the study	313:321	the study of developmental origins of behavior	313:358	Although it is well-established that the immune system plays an important role in the development of physiology and behavior, the gut microbiome has recently become of interest in the study of developmental origins of behavior.
29981426	6	90	with	males	1516:1520	arg1	individual					1547:1556	a novel individual	1539:1556	a novel individual of the same age and sex in adulthood to score same-sex behavior	1539:1620	We tracked physiology across development, and paired males and females with a novel individual of the same age and sex in adulthood to score same-sex behavior (e.g., aggression, investigation, grooming) before antibiotic treatment, immediately following treatment, and after recovery from antibiotics.
29981426	1	91	theme	behavior	351:358	arg1	origins					340:346	developmental origins	326:346	developmental origins of behavior	326:358	Although it is well-established that the immune system plays an important role in the development of physiology and behavior, the gut microbiome has recently become of interest in the study of developmental origins of behavior.
29981426	7	92	theme	smaller	1861:1867	arg1	ovaries					1869:1875	smaller ovaries	1861:1875	smaller ovaries	1861:1875	LPS-treated females exhibited impaired reproductive physiology and function in adulthood (e.g., smaller ovaries and abnormal estrous cycles), and female and male gut microbial communities were strongly affected by antibiotic treatment in adulthood, but only slightly affected by postnatal LPS alone.
29981426	6	93	theme	same	1565:1568	arg1	age					1570:1572	same age	1565:1572	same age	1565:1572	We tracked physiology across development, and paired males and females with a novel individual of the same age and sex in adulthood to score same-sex behavior (e.g., aggression, investigation, grooming) before antibiotic treatment, immediately following treatment, and after recovery from antibiotics.
29981426	2	94	theme	precise	493:499	arg1	nature					501:506	precise nature	493:506	precise nature	493:506	Studies suggest that the effects of early-life immune activation may not occur until a secondary stressor is introduced, though the precise nature and timing of the stressor may be critical in the response.
29981426	5	95	theme	community	1404:1412	arg1	composition					1414:1424	microbial community composition	1394:1424	microbial community composition	1394:1424	In order to test the hypothesis that an early-life immune challenge makes individuals more vulnerable to the effects of antibiotics, we mimicked an early-life infection by injecting pups at postnatal day 3 and 5 with lipopolysaccharide (LPS; cell wall component of gram-negative bacteria) or saline, and subsequently exposed the same animals to antibiotic treatment (known to influence microbial community composition and behavior) or water in adulthood.
29981426	5	96	theme	bacteria	1287:1294	arg1	component					1260:1268	cell wall component	1250:1268	LPS; cell wall component of gram-negative bacteria	1245:1294	In order to test the hypothesis that an early-life immune challenge makes individuals more vulnerable to the effects of antibiotics, we mimicked an early-life infection by injecting pups at postnatal day 3 and 5 with lipopolysaccharide (LPS; cell wall component of gram-negative bacteria) or saline, and subsequently exposed the same animals to antibiotic treatment (known to influence microbial community composition and behavior) or water in adulthood.
29981426	4	97	contain	had	841:843	arg2	consequences					855:866	long-term consequences	845:866	long-term consequences	845:866	Here, we sought to determine whether early-life activation of the immune system had long-term consequences on how the gut microbiome responds to antibiotic treatment in adulthood and whether those changes influence adult same-sex social behavior.
29981426	4	97	contain	had	841:843	arg1	activation					809:818	early-life activation	798:818	early-life activation of the immune system	798:839	Here, we sought to determine whether early-life activation of the immune system had long-term consequences on how the gut microbiome responds to antibiotic treatment in adulthood and whether those changes influence adult same-sex social behavior.
29981426	4	98	theme	adult	976:980	arg1	behavior					998:1005	adult same-sex social behavior	976:1005	adult same-sex social behavior	976:1005	Here, we sought to determine whether early-life activation of the immune system had long-term consequences on how the gut microbiome responds to antibiotic treatment in adulthood and whether those changes influence adult same-sex social behavior.
29981426	2	99	dep	nature	501:506	arg1	the					489:491	the	489:491	the	489:491	Studies suggest that the effects of early-life immune activation may not occur until a secondary stressor is introduced, though the precise nature and timing of the stressor may be critical in the response.
29981426	6	100	theme	novel	1541:1545	arg1	individual					1547:1556	a novel individual	1539:1556	a novel individual of the same age and sex in adulthood to score same-sex behavior	1539:1620	We tracked physiology across development, and paired males and females with a novel individual of the same age and sex in adulthood to score same-sex behavior (e.g., aggression, investigation, grooming) before antibiotic treatment, immediately following treatment, and after recovery from antibiotics.
29981426	4	101	theme	social	991:996	arg1	behavior					998:1005	adult same-sex social behavior	976:1005	adult same-sex social behavior	976:1005	Here, we sought to determine whether early-life activation of the immune system had long-term consequences on how the gut microbiome responds to antibiotic treatment in adulthood and whether those changes influence adult same-sex social behavior.
29981426	10	102	theme	immune	2580:2585	arg1	challenge					2587:2595	an early-life immune challenge	2566:2595	an early-life immune challenge	2566:2595	Collectively, these results provide novel evidence that some of the sex-specific behavioral consequences of an early-life immune challenge may not transpire until an individual is faced with a secondary challenge, and the context in which an individual is exposed can greatly influence the response.
29981426	5	103	theme	wall	1255:1258	arg1	component					1260:1268	cell wall component	1250:1268	LPS; cell wall component of gram-negative bacteria	1245:1294	In order to test the hypothesis that an early-life immune challenge makes individuals more vulnerable to the effects of antibiotics, we mimicked an early-life infection by injecting pups at postnatal day 3 and 5 with lipopolysaccharide (LPS; cell wall component of gram-negative bacteria) or saline, and subsequently exposed the same animals to antibiotic treatment (known to influence microbial community composition and behavior) or water in adulthood.
29981426	2	104	theme	immune	408:413	arg1	activation					415:424	early-life immune activation	397:424	early-life immune activation	397:424	Studies suggest that the effects of early-life immune activation may not occur until a secondary stressor is introduced, though the precise nature and timing of the stressor may be critical in the response.
29981426	8	105	theme	increased	2233:2241	arg1	grooming					2243:2250	increased grooming	2233:2250	increased grooming	2233:2250	Interestingly, LPS-treated males exhibited more robust changes in their behavioral response following adult antibiotic treatment, including decreased investigation and increased grooming, suggestive of changes in anxiety-like behaviors.
29981426	0	106	theme	behavioral	56:65	arg1	changes					67:73	behavioral changes	56:73	behavioral changes following alterations of the gut microbiome in adulthood	56:130	Early-life sickness may predispose Siberian hamsters to behavioral changes following alterations of the gut microbiome in adulthood.
29981426	7	107	dep	ovaries	1869:1875	arg1	e.g.					1855:1858	e.g.	1855:1858	e.g.	1855:1858	LPS-treated females exhibited impaired reproductive physiology and function in adulthood (e.g., smaller ovaries and abnormal estrous cycles), and female and male gut microbial communities were strongly affected by antibiotic treatment in adulthood, but only slightly affected by postnatal LPS alone.
29981426	9	108	from	life	2452:2455	arg1	early					2443:2447	early	2443:2447	early	2443:2447	These data suggest that males may be more vulnerable than females to behavioral abnormalities after being predisposed to an immune challenge early in life.
29981426	7	109	theme	impaired	1795:1802	arg1	physiology					1817:1826	impaired reproductive physiology	1795:1826	impaired reproductive physiology	1795:1826	LPS-treated females exhibited impaired reproductive physiology and function in adulthood (e.g., smaller ovaries and abnormal estrous cycles), and female and male gut microbial communities were strongly affected by antibiotic treatment in adulthood, but only slightly affected by postnatal LPS alone.
29981426	7	110	theme	LPS-treated	1765:1775	arg1	females					1777:1783	LPS-treated females	1765:1783	LPS-treated females	1765:1783	LPS-treated females exhibited impaired reproductive physiology and function in adulthood (e.g., smaller ovaries and abnormal estrous cycles), and female and male gut microbial communities were strongly affected by antibiotic treatment in adulthood, but only slightly affected by postnatal LPS alone.
29981426	3	111	theme	immune	626:631	arg1	system					633:638	the immune system	622:638	the immune system	622:638	Further, recent work suggests that the microbiome and the immune system develop in parallel, and therefore any perturbations to one of these systems early in life will likely affect the other.
29981426	8	112	theme	LPS-treated	2080:2090	arg1	males					2092:2096	LPS-treated males	2080:2096	LPS-treated males	2080:2096	Interestingly, LPS-treated males exhibited more robust changes in their behavioral response following adult antibiotic treatment, including decreased investigation and increased grooming, suggestive of changes in anxiety-like behaviors.
29981426	6	113	from	age	1570:1572	arg1	adulthood					1585:1593	adulthood	1585:1593	adulthood	1585:1593	We tracked physiology across development, and paired males and females with a novel individual of the same age and sex in adulthood to score same-sex behavior (e.g., aggression, investigation, grooming) before antibiotic treatment, immediately following treatment, and after recovery from antibiotics.
29981426	5	114	theme	postnatal	1198:1206	arg1	day					1208:1210	postnatal day 3 and 5	1198:1218	day	1208:1210	In order to test the hypothesis that an early-life immune challenge makes individuals more vulnerable to the effects of antibiotics, we mimicked an early-life infection by injecting pups at postnatal day 3 and 5 with lipopolysaccharide (LPS; cell wall component of gram-negative bacteria) or saline, and subsequently exposed the same animals to antibiotic treatment (known to influence microbial community composition and behavior) or water in adulthood.
31454152	1	0	theme	hemophilia	242:251	arg1	A					253:253	an effective hemophilia A	229:253	an effective hemophilia A treatment for half a century	229:282	BACKGROUND Coagulation factor VIII represents one of the oldest protein-based therapeutics, serving as an effective hemophilia A treatment for half a century.
31454152	8	1	theme	human	1235:1239	arg1	protein					1247:1253	the human FVIII protein	1231:1253	the human FVIII protein	1231:1253	This structure shows remarkable conservation with the human FVIII protein and provides a detailed view of the interface between the A2 domain and the remaining FVIII structure.
31454152	6	2	theme	ET3i	934:937	arg1	structure					921:929	The structure	917:929	The structure of ET3i	917:937	METHODS The structure of ET3i was characterized with X-ray crystallography and tandem mass spectrometry-based glycoproteomics.
31454152	12	3	theme	next-generation	1807:1821	arg1	efforts					1835:1841	next-generation engineering efforts	1807:1841	next-generation engineering efforts to develop FVIII therapeutics with longer half-lives, higher expression levels, and lower immunogenicity	1807:1946	CONCLUSIONS These improved structures yield a more confident model for next-generation engineering efforts to develop FVIII therapeutics with longer half-lives, higher expression levels, and lower immunogenicity.
31454152	11	4	theme	improved	1704:1711	arg1	statistics					1724:1733	improved refinement statistics	1704:1733	improved refinement statistics	1704:1733	With the original datasets at 3.7 Å and 4.0 Å resolution, this new structure resulted in improved refinement statistics.
31454152	10	5	theme	human	1602:1606	arg1	FVIII					1608:1612	human FVIII	1602:1612	human FVIII	1602:1612	The improved model and stereochemistry of ET3i served as a scaffold to generate an improved, refined structure of human FVIII.
31454152	7	6	theme	LC-MS/MS	1155:1162	arg1	glycoproteomics					1164:1178	LC-MS/MS glycoproteomics	1155:1178	LC-MS/MS glycoproteomics	1155:1178	RESULTS Here, we report the 3.2 Å crystal structure of ET3i and characterize the distribution of N-linked glycans with LC-MS/MS glycoproteomics.
31454152	7	7	theme	glycans	1142:1148	arg1	distribution					1117:1128	the distribution	1113:1128	the distribution of N-linked glycans with LC-MS/MS glycoproteomics	1113:1178	RESULTS Here, we report the 3.2 Å crystal structure of ET3i and characterize the distribution of N-linked glycans with LC-MS/MS glycoproteomics.
31454152	11	8	from	resolution	1661:1670	arg1	datasets					1633:1640	the original datasets	1620:1640	the original datasets at 3.7 Å and 4.0 Å resolution	1620:1670	With the original datasets at 3.7 Å and 4.0 Å resolution, this new structure resulted in improved refinement statistics.
31454152	8	9	theme	detailed	1270:1277	arg1	view					1279:1282	a detailed view	1268:1282	a detailed view of the interface between the A2 domain and the remaining FVIII structure	1268:1355	This structure shows remarkable conservation with the human FVIII protein and provides a detailed view of the interface between the A2 domain and the remaining FVIII structure.
31454152	5	10	theme	FVIII	721:725	arg1	construct					708:716	a bioengineered construct	692:716	a bioengineered construct	692:716	OBJECTIVES To structurally characterize a bioengineered construct of FVIII, termed ET3i, which is a human/porcine chimeric B domain-deleted heterodimer with improved expression and slower A2 domain dissociation following proteolytic activation by thrombin.
31454152	5	10	theme	FVIII	721:725	arg1	heterodimer					792:802	a human/porcine chimeric B domain-deleted heterodimer	750:802	a human/porcine chimeric B domain-deleted heterodimer with improved expression and slower A2 domain dissociation following proteolytic activation by thrombin	750:906	OBJECTIVES To structurally characterize a bioengineered construct of FVIII, termed ET3i, which is a human/porcine chimeric B domain-deleted heterodimer with improved expression and slower A2 domain dissociation following proteolytic activation by thrombin.
31454152	1	11	theme	oldest	183:188	arg1	therapeutics					204:215	the oldest protein-based therapeutics	179:215	the oldest protein-based therapeutics	179:215	BACKGROUND Coagulation factor VIII represents one of the oldest protein-based therapeutics, serving as an effective hemophilia A treatment for half a century.
31454152	4	12	theme	therapies	641:649	arg1	development					588:598	the development	584:598	the development of bioengineered FVIII products and gene therapies	584:649	These issues have inspired research into the development of bioengineered FVIII products and gene therapies.
31454152	0	13	theme	C2	115:116	arg1	domain					118:123	the C2 domain	111:123	the C2 domain	111:123	The 3.2 Å structure of a bioengineered variant of blood coagulation factor VIII indicates two conformations of the C2 domain.
31454152	7	14	theme	3.2 Å	1064:1068	arg1	structure					1078:1086	the 3.2 Å crystal structure	1060:1086	the 3.2 Å crystal structure of ET3i	1060:1094	RESULTS Here, we report the 3.2 Å crystal structure of ET3i and characterize the distribution of N-linked glycans with LC-MS/MS glycoproteomics.
31454152	5	15	theme	proteolytic	873:883	arg1	activation					885:894	proteolytic activation	873:894	proteolytic activation by thrombin	873:906	OBJECTIVES To structurally characterize a bioengineered construct of FVIII, termed ET3i, which is a human/porcine chimeric B domain-deleted heterodimer with improved expression and slower A2 domain dissociation following proteolytic activation by thrombin.
31454152	8	16	theme	interface	1291:1299	arg1	view					1279:1282	a detailed view	1268:1282	a detailed view of the interface between the A2 domain and the remaining FVIII structure	1268:1355	This structure shows remarkable conservation with the human FVIII protein and provides a detailed view of the interface between the A2 domain and the remaining FVIII structure.
31454152	6	17	theme	X-ray	962:966	arg1	crystallography					968:982	X-ray crystallography	962:982	X-ray crystallography	962:982	METHODS The structure of ET3i was characterized with X-ray crystallography and tandem mass spectrometry-based glycoproteomics.
31454152	9	18	theme	remaining	1461:1469	arg1	structure					1477:1485	the remaining FVIII structure	1457:1485	the remaining FVIII structure	1457:1485	With two FVIII molecules in the crystal, we observe two conformations of the C2 domain relative to the remaining FVIII structure.
31454152	9	19	theme	FVIII	1471:1475	arg1	structure					1477:1485	the remaining FVIII structure	1457:1485	the remaining FVIII structure	1457:1485	With two FVIII molecules in the crystal, we observe two conformations of the C2 domain relative to the remaining FVIII structure.
31454152	5	20	theme	A2	840:841	arg1	dissociation					850:861	slower A2 domain dissociation	833:861	slower A2 domain dissociation	833:861	OBJECTIVES To structurally characterize a bioengineered construct of FVIII, termed ET3i, which is a human/porcine chimeric B domain-deleted heterodimer with improved expression and slower A2 domain dissociation following proteolytic activation by thrombin.
31454152	1	21	theme	BACKGROUND	126:135	arg1	VIII					156:159	BACKGROUND Coagulation factor VIII	126:159	BACKGROUND Coagulation factor VIII	126:159	BACKGROUND Coagulation factor VIII represents one of the oldest protein-based therapeutics, serving as an effective hemophilia A treatment for half a century.
31454152	12	22	dep	efforts	1835:1841	arg1	develop					1846:1852	develop	1846:1852	to develop FVIII therapeutics with longer half-lives, higher expression levels, and lower immunogenicity	1843:1946	CONCLUSIONS These improved structures yield a more confident model for next-generation engineering efforts to develop FVIII therapeutics with longer half-lives, higher expression levels, and lower immunogenicity.
31454152	0	23	theme	coagulation	56:66	arg1	VIII					75:78	blood coagulation factor VIII	50:78	blood coagulation factor VIII	50:78	The 3.2 Å structure of a bioengineered variant of blood coagulation factor VIII indicates two conformations of the C2 domain.
31454152	1	24	theme	factor	149:154	arg1	VIII					156:159	BACKGROUND Coagulation factor VIII	126:159	BACKGROUND Coagulation factor VIII	126:159	BACKGROUND Coagulation factor VIII represents one of the oldest protein-based therapeutics, serving as an effective hemophilia A treatment for half a century.
31454152	8	25	with	conservation	1213:1224	arg1	protein					1247:1253	the human FVIII protein	1231:1253	the human FVIII protein	1231:1253	This structure shows remarkable conservation with the human FVIII protein and provides a detailed view of the interface between the A2 domain and the remaining FVIII structure.
31454152	0	26	theme	VIII	75:78	arg1	variant					39:45	a bioengineered variant	23:45	a bioengineered variant of blood coagulation factor VIII	23:78	The 3.2 Å structure of a bioengineered variant of blood coagulation factor VIII indicates two conformations of the C2 domain.
31454152	9	27	theme	FVIII	1367:1371	arg1	molecules					1373:1381	two FVIII molecules	1363:1381	two FVIII molecules in the crystal	1363:1396	With two FVIII molecules in the crystal, we observe two conformations of the C2 domain relative to the remaining FVIII structure.
31454152	10	28	theme	ET3i	1530:1533	arg1	scaffold					1547:1554	a scaffold	1545:1554	a scaffold to generate an improved, refined structure of human FVIII	1545:1612	The improved model and stereochemistry of ET3i served as a scaffold to generate an improved, refined structure of human FVIII.
31454152	10	28	theme	ET3i	1530:1533	arg1	stereochemistry					1511:1525	stereochemistry	1511:1525	stereochemistry	1511:1525	The improved model and stereochemistry of ET3i served as a scaffold to generate an improved, refined structure of human FVIII.
31454152	10	28	theme	ET3i	1530:1533	arg1	model					1501:1505	The improved model	1488:1505	The improved model	1488:1505	The improved model and stereochemistry of ET3i served as a scaffold to generate an improved, refined structure of human FVIII.
31454152	3	29	theme	overall	414:420	arg1	success					432:438	overall treatment success	414:438	overall treatment success	414:438	Despite overall treatment success, significant limitations remain, including treatment invasiveness, duration, immunogenicity, and cost.
31454152	3	30	theme	significant	441:451	arg1	limitations					453:463	significant limitations	441:463	significant limitations	441:463	Despite overall treatment success, significant limitations remain, including treatment invasiveness, duration, immunogenicity, and cost.
31454152	0	31	theme	Å	8:8	arg1	structure					10:18	The 3.2 Å structure	0:18	The 3.2 Å structure of a bioengineered variant of blood coagulation factor VIII	0:78	The 3.2 Å structure of a bioengineered variant of blood coagulation factor VIII indicates two conformations of the C2 domain.
31454152	1	32	theme	a	274:274	arg1	century					276:282	half a century	269:282	half a century	269:282	BACKGROUND Coagulation factor VIII represents one of the oldest protein-based therapeutics, serving as an effective hemophilia A treatment for half a century.
31454152	12	33	theme	lower	1927:1931	arg1	immunogenicity					1933:1946	lower immunogenicity	1927:1946	lower immunogenicity	1927:1946	CONCLUSIONS These improved structures yield a more confident model for next-generation engineering efforts to develop FVIII therapeutics with longer half-lives, higher expression levels, and lower immunogenicity.
31454152	4	34	theme	gene	636:639	arg1	therapies					641:649	gene therapies	636:649	gene therapies	636:649	These issues have inspired research into the development of bioengineered FVIII products and gene therapies.
31454152	10	35	theme	improved	1571:1578	arg1	structure					1589:1597	an improved, refined structure	1568:1597	structure	1589:1597	The improved model and stereochemistry of ET3i served as a scaffold to generate an improved, refined structure of human FVIII.
31454152	5	36	theme	human/porcine	752:764	arg1	construct					708:716	a bioengineered construct	692:716	a bioengineered construct	692:716	OBJECTIVES To structurally characterize a bioengineered construct of FVIII, termed ET3i, which is a human/porcine chimeric B domain-deleted heterodimer with improved expression and slower A2 domain dissociation following proteolytic activation by thrombin.
31454152	5	36	theme	human/porcine	752:764	arg1	heterodimer					792:802	a human/porcine chimeric B domain-deleted heterodimer	750:802	a human/porcine chimeric B domain-deleted heterodimer with improved expression and slower A2 domain dissociation following proteolytic activation by thrombin	750:906	OBJECTIVES To structurally characterize a bioengineered construct of FVIII, termed ET3i, which is a human/porcine chimeric B domain-deleted heterodimer with improved expression and slower A2 domain dissociation following proteolytic activation by thrombin.
31454152	5	37	theme	improved	809:816	arg1	expression					818:827	improved expression	809:827	improved expression	809:827	OBJECTIVES To structurally characterize a bioengineered construct of FVIII, termed ET3i, which is a human/porcine chimeric B domain-deleted heterodimer with improved expression and slower A2 domain dissociation following proteolytic activation by thrombin.
31454152	12	38	theme	expression	1904:1913	arg1	levels					1915:1920	higher expression levels	1897:1920	higher expression levels	1897:1920	CONCLUSIONS These improved structures yield a more confident model for next-generation engineering efforts to develop FVIII therapeutics with longer half-lives, higher expression levels, and lower immunogenicity.
31454152	12	39	theme	higher	1897:1902	arg1	levels					1915:1920	higher expression levels	1897:1920	higher expression levels	1897:1920	CONCLUSIONS These improved structures yield a more confident model for next-generation engineering efforts to develop FVIII therapeutics with longer half-lives, higher expression levels, and lower immunogenicity.
31454152	11	40	theme	original	1624:1631	arg1	datasets					1633:1640	the original datasets	1620:1640	the original datasets at 3.7 Å and 4.0 Å resolution	1620:1670	With the original datasets at 3.7 Å and 4.0 Å resolution, this new structure resulted in improved refinement statistics.
31454152	2	41	theme	repeated	315:322	arg1	infusions					336:344	repeated intravenous infusions	315:344	repeated intravenous infusions of blood coagulation factor VIII (FVIII)	315:385	Optimal treatment consists of repeated intravenous infusions of blood coagulation factor VIII (FVIII) per week for life.
31454152	9	42	theme	C2	1435:1436	arg1	domain					1438:1443	the C2 domain	1431:1443	the C2 domain relative to the remaining FVIII structure	1431:1485	With two FVIII molecules in the crystal, we observe two conformations of the C2 domain relative to the remaining FVIII structure.
31454152	12	43	theme	FVIII	1854:1858	arg1	therapeutics					1860:1871	FVIII therapeutics	1854:1871	FVIII therapeutics with longer half-lives, higher expression levels, and lower immunogenicity	1854:1946	CONCLUSIONS These improved structures yield a more confident model for next-generation engineering efforts to develop FVIII therapeutics with longer half-lives, higher expression levels, and lower immunogenicity.
31454152	6	44	dep	METHODS	909:915	arg1	characterized					943:955	characterized	943:955	was characterized with X-ray crystallography and tandem mass spectrometry-based glycoproteomics	939:1033	METHODS The structure of ET3i was characterized with X-ray crystallography and tandem mass spectrometry-based glycoproteomics.
31454152	2	45	theme	factor	367:372	arg1	FVIII					380:384	FVIII	380:384	FVIII	380:384	Optimal treatment consists of repeated intravenous infusions of blood coagulation factor VIII (FVIII) per week for life.
31454152	2	45	theme	factor	367:372	arg1	VIII					374:377	blood coagulation factor VIII	349:377	blood coagulation factor VIII (FVIII)	349:385	Optimal treatment consists of repeated intravenous infusions of blood coagulation factor VIII (FVIII) per week for life.
31454152	9	46	theme	relative	1445:1452	arg1	domain					1438:1443	the C2 domain	1431:1443	the C2 domain relative to the remaining FVIII structure	1431:1485	With two FVIII molecules in the crystal, we observe two conformations of the C2 domain relative to the remaining FVIII structure.
31454152	4	47	theme	bioengineered	603:615	arg1	products					623:630	bioengineered FVIII products	603:630	bioengineered FVIII products	603:630	These issues have inspired research into the development of bioengineered FVIII products and gene therapies.
31454152	10	48	theme	refined	1581:1587	arg1	structure					1589:1597	an improved, refined structure	1568:1597	structure	1589:1597	The improved model and stereochemistry of ET3i served as a scaffold to generate an improved, refined structure of human FVIII.
31454152	5	49	theme	chimeric	766:773	arg1	construct					708:716	a bioengineered construct	692:716	a bioengineered construct	692:716	OBJECTIVES To structurally characterize a bioengineered construct of FVIII, termed ET3i, which is a human/porcine chimeric B domain-deleted heterodimer with improved expression and slower A2 domain dissociation following proteolytic activation by thrombin.
31454152	5	49	theme	chimeric	766:773	arg1	heterodimer					792:802	a human/porcine chimeric B domain-deleted heterodimer	750:802	a human/porcine chimeric B domain-deleted heterodimer with improved expression and slower A2 domain dissociation following proteolytic activation by thrombin	750:906	OBJECTIVES To structurally characterize a bioengineered construct of FVIII, termed ET3i, which is a human/porcine chimeric B domain-deleted heterodimer with improved expression and slower A2 domain dissociation following proteolytic activation by thrombin.
31454152	2	50	theme	blood	349:353	arg1	FVIII					380:384	FVIII	380:384	FVIII	380:384	Optimal treatment consists of repeated intravenous infusions of blood coagulation factor VIII (FVIII) per week for life.
31454152	2	50	theme	blood	349:353	arg1	VIII					374:377	blood coagulation factor VIII	349:377	blood coagulation factor VIII (FVIII)	349:385	Optimal treatment consists of repeated intravenous infusions of blood coagulation factor VIII (FVIII) per week for life.
31454152	1	51	theme	effective	232:240	arg1	A					253:253	an effective hemophilia A	229:253	an effective hemophilia A treatment for half a century	229:282	BACKGROUND Coagulation factor VIII represents one of the oldest protein-based therapeutics, serving as an effective hemophilia A treatment for half a century.
31454152	12	52	theme	engineering	1823:1833	arg1	efforts					1835:1841	next-generation engineering efforts	1807:1841	next-generation engineering efforts to develop FVIII therapeutics with longer half-lives, higher expression levels, and lower immunogenicity	1807:1946	CONCLUSIONS These improved structures yield a more confident model for next-generation engineering efforts to develop FVIII therapeutics with longer half-lives, higher expression levels, and lower immunogenicity.
31454152	12	53	dep	CONCLUSIONS	1736:1746	arg1	yield					1774:1778	yield	1774:1778	yield a more confident model for next-generation engineering efforts to develop FVIII therapeutics with longer half-lives, higher expression levels, and lower immunogenicity	1774:1946	CONCLUSIONS These improved structures yield a more confident model for next-generation engineering efforts to develop FVIII therapeutics with longer half-lives, higher expression levels, and lower immunogenicity.
31454152	1	54	theme	A	253:253	arg1	treatment					255:263	an effective hemophilia A treatment	229:263	an effective hemophilia A treatment for half a century	229:282	BACKGROUND Coagulation factor VIII represents one of the oldest protein-based therapeutics, serving as an effective hemophilia A treatment for half a century.
31454152	10	55	theme	FVIII	1608:1612	arg1	structure					1589:1597	an improved, refined structure	1568:1597	structure	1589:1597	The improved model and stereochemistry of ET3i served as a scaffold to generate an improved, refined structure of human FVIII.
31454152	11	56	theme	refinement	1713:1722	arg1	statistics					1724:1733	improved refinement statistics	1704:1733	improved refinement statistics	1704:1733	With the original datasets at 3.7 Å and 4.0 Å resolution, this new structure resulted in improved refinement statistics.
31454152	8	57	theme	FVIII	1241:1245	arg1	protein					1247:1253	the human FVIII protein	1231:1253	the human FVIII protein	1231:1253	This structure shows remarkable conservation with the human FVIII protein and provides a detailed view of the interface between the A2 domain and the remaining FVIII structure.
31454152	12	58	theme	confident	1787:1795	arg1	model					1797:1801	a more confident model	1780:1801	a more confident model for next-generation engineering efforts to develop FVIII therapeutics with longer half-lives, higher expression levels, and lower immunogenicity	1780:1946	CONCLUSIONS These improved structures yield a more confident model for next-generation engineering efforts to develop FVIII therapeutics with longer half-lives, higher expression levels, and lower immunogenicity.
31454152	5	59	with	heterodimer	792:802	arg1	dissociation					850:861	slower A2 domain dissociation	833:861	slower A2 domain dissociation	833:861	OBJECTIVES To structurally characterize a bioengineered construct of FVIII, termed ET3i, which is a human/porcine chimeric B domain-deleted heterodimer with improved expression and slower A2 domain dissociation following proteolytic activation by thrombin.
31454152	5	59	with	heterodimer	792:802	arg1	expression					818:827	improved expression	809:827	improved expression	809:827	OBJECTIVES To structurally characterize a bioengineered construct of FVIII, termed ET3i, which is a human/porcine chimeric B domain-deleted heterodimer with improved expression and slower A2 domain dissociation following proteolytic activation by thrombin.
31454152	5	60	theme	B	775:775	arg1	construct					708:716	a bioengineered construct	692:716	a bioengineered construct	692:716	OBJECTIVES To structurally characterize a bioengineered construct of FVIII, termed ET3i, which is a human/porcine chimeric B domain-deleted heterodimer with improved expression and slower A2 domain dissociation following proteolytic activation by thrombin.
31454152	5	60	theme	B	775:775	arg1	heterodimer					792:802	a human/porcine chimeric B domain-deleted heterodimer	750:802	a human/porcine chimeric B domain-deleted heterodimer with improved expression and slower A2 domain dissociation following proteolytic activation by thrombin	750:906	OBJECTIVES To structurally characterize a bioengineered construct of FVIII, termed ET3i, which is a human/porcine chimeric B domain-deleted heterodimer with improved expression and slower A2 domain dissociation following proteolytic activation by thrombin.
31454152	7	61	theme	N-linked	1133:1140	arg1	glycans					1142:1148	N-linked glycans	1133:1148	N-linked glycans	1133:1148	RESULTS Here, we report the 3.2 Å crystal structure of ET3i and characterize the distribution of N-linked glycans with LC-MS/MS glycoproteomics.
31454152	0	62	theme	bioengineered	25:37	arg1	variant					39:45	a bioengineered variant	23:45	a bioengineered variant of blood coagulation factor VIII	23:78	The 3.2 Å structure of a bioengineered variant of blood coagulation factor VIII indicates two conformations of the C2 domain.
31454152	8	63	theme	A2	1313:1314	arg1	domain					1316:1321	the A2 domain	1309:1321	the A2 domain	1309:1321	This structure shows remarkable conservation with the human FVIII protein and provides a detailed view of the interface between the A2 domain and the remaining FVIII structure.
31454152	2	64	theme	Optimal	285:291	arg1	treatment					293:301	Optimal treatment	285:301	Optimal treatment	285:301	Optimal treatment consists of repeated intravenous infusions of blood coagulation factor VIII (FVIII) per week for life.
31454152	5	65	theme	domain	843:848	arg1	dissociation					850:861	slower A2 domain dissociation	833:861	slower A2 domain dissociation	833:861	OBJECTIVES To structurally characterize a bioengineered construct of FVIII, termed ET3i, which is a human/porcine chimeric B domain-deleted heterodimer with improved expression and slower A2 domain dissociation following proteolytic activation by thrombin.
31454152	9	66	from	molecules	1373:1381	arg1	crystal					1390:1396	the crystal	1386:1396	the crystal	1386:1396	With two FVIII molecules in the crystal, we observe two conformations of the C2 domain relative to the remaining FVIII structure.
31454152	6	67	theme	spectrometry-based	1000:1017	arg1	glycoproteomics					1019:1033	tandem mass spectrometry-based glycoproteomics	988:1033	tandem mass spectrometry-based glycoproteomics	988:1033	METHODS The structure of ET3i was characterized with X-ray crystallography and tandem mass spectrometry-based glycoproteomics.
31454152	4	68	theme	products	623:630	arg1	development					588:598	the development	584:598	the development of bioengineered FVIII products and gene therapies	584:649	These issues have inspired research into the development of bioengineered FVIII products and gene therapies.
31454152	2	69	theme	intravenous	324:334	arg1	infusions					336:344	repeated intravenous infusions	315:344	repeated intravenous infusions of blood coagulation factor VIII (FVIII)	315:385	Optimal treatment consists of repeated intravenous infusions of blood coagulation factor VIII (FVIII) per week for life.
31454152	0	70	theme	domain	118:123	arg1	conformations					94:106	two conformations	90:106	two conformations of the C2 domain	90:123	The 3.2 Å structure of a bioengineered variant of blood coagulation factor VIII indicates two conformations of the C2 domain.
31454152	1	71	theme	protein-based	190:202	arg1	therapeutics					204:215	the oldest protein-based therapeutics	179:215	the oldest protein-based therapeutics	179:215	BACKGROUND Coagulation factor VIII represents one of the oldest protein-based therapeutics, serving as an effective hemophilia A treatment for half a century.
31454152	6	72	theme	tandem	988:993	arg1	glycoproteomics					1019:1033	tandem mass spectrometry-based glycoproteomics	988:1033	tandem mass spectrometry-based glycoproteomics	988:1033	METHODS The structure of ET3i was characterized with X-ray crystallography and tandem mass spectrometry-based glycoproteomics.
31454152	8	73	theme	remaining	1331:1339	arg1	structure					1347:1355	the remaining FVIII structure	1327:1355	the remaining FVIII structure	1327:1355	This structure shows remarkable conservation with the human FVIII protein and provides a detailed view of the interface between the A2 domain and the remaining FVIII structure.
31454152	7	74	theme	ET3i	1091:1094	arg1	structure					1078:1086	the 3.2 Å crystal structure	1060:1086	the 3.2 Å crystal structure of ET3i	1060:1094	RESULTS Here, we report the 3.2 Å crystal structure of ET3i and characterize the distribution of N-linked glycans with LC-MS/MS glycoproteomics.
31454152	7	75	theme	crystal	1070:1076	arg1	structure					1078:1086	the 3.2 Å crystal structure	1060:1086	the 3.2 Å crystal structure of ET3i	1060:1094	RESULTS Here, we report the 3.2 Å crystal structure of ET3i and characterize the distribution of N-linked glycans with LC-MS/MS glycoproteomics.
31454152	5	76	theme	bioengineered	694:706	arg1	construct					708:716	a bioengineered construct	692:716	a bioengineered construct	692:716	OBJECTIVES To structurally characterize a bioengineered construct of FVIII, termed ET3i, which is a human/porcine chimeric B domain-deleted heterodimer with improved expression and slower A2 domain dissociation following proteolytic activation by thrombin.
31454152	5	76	theme	bioengineered	694:706	arg1	heterodimer					792:802	a human/porcine chimeric B domain-deleted heterodimer	750:802	a human/porcine chimeric B domain-deleted heterodimer with improved expression and slower A2 domain dissociation following proteolytic activation by thrombin	750:906	OBJECTIVES To structurally characterize a bioengineered construct of FVIII, termed ET3i, which is a human/porcine chimeric B domain-deleted heterodimer with improved expression and slower A2 domain dissociation following proteolytic activation by thrombin.
31454152	12	77	theme	improved	1754:1761	arg1	structures					1763:1772	These improved structures	1748:1772	These improved structures	1748:1772	CONCLUSIONS These improved structures yield a more confident model for next-generation engineering efforts to develop FVIII therapeutics with longer half-lives, higher expression levels, and lower immunogenicity.
31454152	0	78	theme	blood	50:54	arg1	VIII					75:78	blood coagulation factor VIII	50:78	blood coagulation factor VIII	50:78	The 3.2 Å structure of a bioengineered variant of blood coagulation factor VIII indicates two conformations of the C2 domain.
31454152	0	79	theme	factor	68:73	arg1	VIII					75:78	blood coagulation factor VIII	50:78	blood coagulation factor VIII	50:78	The 3.2 Å structure of a bioengineered variant of blood coagulation factor VIII indicates two conformations of the C2 domain.
31454152	1	80	theme	Coagulation	137:147	arg1	VIII					156:159	BACKGROUND Coagulation factor VIII	126:159	BACKGROUND Coagulation factor VIII	126:159	BACKGROUND Coagulation factor VIII represents one of the oldest protein-based therapeutics, serving as an effective hemophilia A treatment for half a century.
31454152	10	81	theme	improved	1492:1499	arg1	scaffold					1547:1554	a scaffold	1545:1554	a scaffold to generate an improved, refined structure of human FVIII	1545:1612	The improved model and stereochemistry of ET3i served as a scaffold to generate an improved, refined structure of human FVIII.
31454152	10	81	theme	improved	1492:1499	arg1	stereochemistry					1511:1525	stereochemistry	1511:1525	stereochemistry	1511:1525	The improved model and stereochemistry of ET3i served as a scaffold to generate an improved, refined structure of human FVIII.
31454152	10	81	theme	improved	1492:1499	arg1	model					1501:1505	The improved model	1488:1505	The improved model	1488:1505	The improved model and stereochemistry of ET3i served as a scaffold to generate an improved, refined structure of human FVIII.
31454152	12	82	with	therapeutics	1860:1871	arg1	half-lives					1885:1894	longer half-lives	1878:1894	longer half-lives	1878:1894	CONCLUSIONS These improved structures yield a more confident model for next-generation engineering efforts to develop FVIII therapeutics with longer half-lives, higher expression levels, and lower immunogenicity.
31454152	12	82	with	therapeutics	1860:1871	arg1	levels					1915:1920	higher expression levels	1897:1920	higher expression levels	1897:1920	CONCLUSIONS These improved structures yield a more confident model for next-generation engineering efforts to develop FVIII therapeutics with longer half-lives, higher expression levels, and lower immunogenicity.
31454152	12	82	with	therapeutics	1860:1871	arg1	immunogenicity					1933:1946	lower immunogenicity	1927:1946	lower immunogenicity	1927:1946	CONCLUSIONS These improved structures yield a more confident model for next-generation engineering efforts to develop FVIII therapeutics with longer half-lives, higher expression levels, and lower immunogenicity.
31454152	3	83	theme	treatment	422:430	arg1	success					432:438	overall treatment success	414:438	overall treatment success	414:438	Despite overall treatment success, significant limitations remain, including treatment invasiveness, duration, immunogenicity, and cost.
31454152	7	84	dep	RESULTS	1036:1042	arg1	characterize					1100:1111	characterize	1100:1111	characterize the distribution of N-linked glycans with LC-MS/MS glycoproteomics	1100:1178	RESULTS Here, we report the 3.2 Å crystal structure of ET3i and characterize the distribution of N-linked glycans with LC-MS/MS glycoproteomics.
31454152	7	84	dep	RESULTS	1036:1042	arg1	report					1053:1058	report	1053:1058	report the 3.2 Å crystal structure of ET3i	1053:1094	RESULTS Here, we report the 3.2 Å crystal structure of ET3i and characterize the distribution of N-linked glycans with LC-MS/MS glycoproteomics.
31454152	2	85	theme	VIII	374:377	arg1	infusions					336:344	repeated intravenous infusions	315:344	repeated intravenous infusions of blood coagulation factor VIII (FVIII)	315:385	Optimal treatment consists of repeated intravenous infusions of blood coagulation factor VIII (FVIII) per week for life.
31454152	5	86	theme	slower	833:838	arg1	dissociation					850:861	slower A2 domain dissociation	833:861	slower A2 domain dissociation	833:861	OBJECTIVES To structurally characterize a bioengineered construct of FVIII, termed ET3i, which is a human/porcine chimeric B domain-deleted heterodimer with improved expression and slower A2 domain dissociation following proteolytic activation by thrombin.
31454152	1	87	theme	half	269:272	arg1	century					276:282	half a century	269:282	half a century	269:282	BACKGROUND Coagulation factor VIII represents one of the oldest protein-based therapeutics, serving as an effective hemophilia A treatment for half a century.
31454152	5	88	theme	domain-deleted	777:790	arg1	construct					708:716	a bioengineered construct	692:716	a bioengineered construct	692:716	OBJECTIVES To structurally characterize a bioengineered construct of FVIII, termed ET3i, which is a human/porcine chimeric B domain-deleted heterodimer with improved expression and slower A2 domain dissociation following proteolytic activation by thrombin.
31454152	5	88	theme	domain-deleted	777:790	arg1	heterodimer					792:802	a human/porcine chimeric B domain-deleted heterodimer	750:802	a human/porcine chimeric B domain-deleted heterodimer with improved expression and slower A2 domain dissociation following proteolytic activation by thrombin	750:906	OBJECTIVES To structurally characterize a bioengineered construct of FVIII, termed ET3i, which is a human/porcine chimeric B domain-deleted heterodimer with improved expression and slower A2 domain dissociation following proteolytic activation by thrombin.
31454152	8	89	theme	FVIII	1341:1345	arg1	structure					1347:1355	the remaining FVIII structure	1327:1355	the remaining FVIII structure	1327:1355	This structure shows remarkable conservation with the human FVIII protein and provides a detailed view of the interface between the A2 domain and the remaining FVIII structure.
31454152	7	90	with	distribution	1117:1128	arg1	glycoproteomics					1164:1178	LC-MS/MS glycoproteomics	1155:1178	LC-MS/MS glycoproteomics	1155:1178	RESULTS Here, we report the 3.2 Å crystal structure of ET3i and characterize the distribution of N-linked glycans with LC-MS/MS glycoproteomics.
31454152	7	91	link	N-linked	1133:1140	arg1	glycans					1142:1148	N-linked glycans	1133:1148	N-linked glycans	1133:1148	RESULTS Here, we report the 3.2 Å crystal structure of ET3i and characterize the distribution of N-linked glycans with LC-MS/MS glycoproteomics.
31454152	0	92	theme	variant	39:45	arg1	structure					10:18	The 3.2 Å structure	0:18	The 3.2 Å structure of a bioengineered variant of blood coagulation factor VIII	0:78	The 3.2 Å structure of a bioengineered variant of blood coagulation factor VIII indicates two conformations of the C2 domain.
31454152	12	93	theme	longer	1878:1883	arg1	half-lives					1885:1894	longer half-lives	1878:1894	longer half-lives	1878:1894	CONCLUSIONS These improved structures yield a more confident model for next-generation engineering efforts to develop FVIII therapeutics with longer half-lives, higher expression levels, and lower immunogenicity.
31454152	3	94	theme	treatment	483:491	arg1	invasiveness					493:504	treatment invasiveness	483:504	treatment invasiveness	483:504	Despite overall treatment success, significant limitations remain, including treatment invasiveness, duration, immunogenicity, and cost.
31454152	9	95	theme	domain	1438:1443	arg1	conformations					1414:1426	two conformations	1410:1426	two conformations of the C2 domain relative to the remaining FVIII structure	1410:1485	With two FVIII molecules in the crystal, we observe two conformations of the C2 domain relative to the remaining FVIII structure.
31454152	8	96	theme	remarkable	1202:1211	arg1	conservation					1213:1224	remarkable conservation	1202:1224	remarkable conservation with the human FVIII protein	1202:1253	This structure shows remarkable conservation with the human FVIII protein and provides a detailed view of the interface between the A2 domain and the remaining FVIII structure.
31454152	1	97	theme	therapeutics	204:215	arg1	one					172:174	one	172:174	one	172:174	BACKGROUND Coagulation factor VIII represents one of the oldest protein-based therapeutics, serving as an effective hemophilia A treatment for half a century.
31454152	1	97	theme	therapeutics	204:215	arg1	therapeutics					204:215	the oldest protein-based therapeutics	179:215	the oldest protein-based therapeutics	179:215	BACKGROUND Coagulation factor VIII represents one of the oldest protein-based therapeutics, serving as an effective hemophilia A treatment for half a century.
31454152	4	98	theme	FVIII	617:621	arg1	products					623:630	bioengineered FVIII products	603:630	bioengineered FVIII products	603:630	These issues have inspired research into the development of bioengineered FVIII products and gene therapies.
31454152	11	99	theme	new	1678:1680	arg1	structure					1682:1690	this new structure	1673:1690	this new structure	1673:1690	With the original datasets at 3.7 Å and 4.0 Å resolution, this new structure resulted in improved refinement statistics.
31454152	2	100	theme	coagulation	355:365	arg1	FVIII					380:384	FVIII	380:384	FVIII	380:384	Optimal treatment consists of repeated intravenous infusions of blood coagulation factor VIII (FVIII) per week for life.
31454152	2	100	theme	coagulation	355:365	arg1	VIII					374:377	blood coagulation factor VIII	349:377	blood coagulation factor VIII (FVIII)	349:385	Optimal treatment consists of repeated intravenous infusions of blood coagulation factor VIII (FVIII) per week for life.
30657063	11	0	from	SECS	1986:1989	arg1	production					1908:1917	the cellulase production	1894:1917	the cellulase production	1894:1917	This strategy also improved the cellulase production, enzymatic hydrolysis yield and the efficiency of the process from SECS to glucose in the context of on-site cellulase production.
30657063	11	0	from	SECS	1986:1989	arg1	yield					1941:1945	enzymatic hydrolysis yield	1920:1945	enzymatic hydrolysis yield	1920:1945	This strategy also improved the cellulase production, enzymatic hydrolysis yield and the efficiency of the process from SECS to glucose in the context of on-site cellulase production.
30657063	11	0	from	SECS	1986:1989	arg1	efficiency					1955:1964	the efficiency	1951:1964	the efficiency of the process from SECS to glucose	1951:2000	This strategy also improved the cellulase production, enzymatic hydrolysis yield and the efficiency of the process from SECS to glucose in the context of on-site cellulase production.
30657063	11	0	from	SECS	1986:1989	arg1	process					1973:1979	the process	1969:1979	the process from SECS	1969:1989	This strategy also improved the cellulase production, enzymatic hydrolysis yield and the efficiency of the process from SECS to glucose in the context of on-site cellulase production.
30657063	3	1	from	synergism	530:538	arg1	cellulase					553:561	T. reesei cellulase	543:561	T. reesei cellulase	543:561	This work aimed at solving this problem by simultaneous enhancement of the beta-exo synergism and exo-exo synergism in T. reesei cellulase to increase the cellulose degrading capability, i.e. enhanced co-expression of the β-glucosidase gene the cellobiohydrolase II gene of T. reesei.
30657063	4	2	from	co-expression	726:738	arg1	reesei					806:811	T. reesei	803:811	T. reesei using the strong promoter Pcbh1	803:843	RESULTS Enhanced co-expression of the β-glucosidase gene and the cellobiohydrolase II gene in T. reesei using the strong promoter Pcbh1 was found successful in overcoming the two weaknesses.
30657063	1	3	theme	biorefineries	270:282	arg1	costs					236:240	the high production costs	216:240	the high production costs of the lignocellulose-based biorefineries	216:282	BACKGROUND Cellulase is the one of the largest contributors to the high production costs of the lignocellulose-based biorefineries.
30657063	10	4	from	gene	1678:1681	arg1	reesei					1689:1694	T. reesei	1686:1694	T. reesei using the strong promoter Pcbh1 in T. reesei	1686:1739	CONCLUSIONS Enhanced co-expression of the β-glucosidase gene and the cellobiohydrolase II gene in T. reesei using the strong promoter Pcbh1 in T. reesei was proven triumphal in the simultaneous enhancement of the beta-exo synergism and exo-exo synergism in T. reesei cellulase.
30657063	11	5	theme	hydrolysis	1930:1939	arg1	yield					1941:1945	enzymatic hydrolysis yield	1920:1945	enzymatic hydrolysis yield	1920:1945	This strategy also improved the cellulase production, enzymatic hydrolysis yield and the efficiency of the process from SECS to glucose in the context of on-site cellulase production.
30657063	12	6	theme	composition	2102:2112	arg1	optimization					2114:2125	the cellulase composition optimization	2088:2125	the cellulase composition optimization	2088:2125	This work is a commendable attempt in the cellulase composition optimization at the transcriptional level.
30657063	5	7	theme	paper	907:911	arg1	activities					913:922	Filter paper activities	900:922	Filter paper activities of T. reesei cellulase	900:945	Filter paper activities of T. reesei cellulase were greatly elevated, which were 7.21 ± 0.45 (E7, Aabgl1 and Trcbh2) and 7.69 ± 0.42 (F6, Anbgl1 and Trcbh2) FPIU/mL.
30657063	4	8	theme	RESULTS	709:715	arg1	co-expression					726:738	RESULTS Enhanced co-expression	709:738	RESULTS Enhanced co-expression of the β-glucosidase gene and the cellobiohydrolase II gene in T. reesei using the strong promoter Pcbh1	709:843	RESULTS Enhanced co-expression of the β-glucosidase gene and the cellobiohydrolase II gene in T. reesei using the strong promoter Pcbh1 was found successful in overcoming the two weaknesses.
30657063	1	9	theme	largest	192:198	arg1	contributors					200:211	the largest contributors	188:211	the largest contributors to the high production costs of the lignocellulose-based biorefineries	188:282	BACKGROUND Cellulase is the one of the largest contributors to the high production costs of the lignocellulose-based biorefineries.
30657063	11	10	theme	production	2038:2047	arg1	context					2009:2015	the context	2005:2015	the context of on-site cellulase production	2005:2047	This strategy also improved the cellulase production, enzymatic hydrolysis yield and the efficiency of the process from SECS to glucose in the context of on-site cellulase production.
30657063	11	11	from	yield	1941:1945	arg1	SECS					1986:1989	SECS	1986:1989	SECS	1986:1989	This strategy also improved the cellulase production, enzymatic hydrolysis yield and the efficiency of the process from SECS to glucose in the context of on-site cellulase production.
30657063	5	12	dep	7.69 ± 0.42	1021:1031	arg1	Trcbh2					1049:1054	Trcbh2	1049:1054	Trcbh2	1049:1054	Filter paper activities of T. reesei cellulase were greatly elevated, which were 7.21 ± 0.45 (E7, Aabgl1 and Trcbh2) and 7.69 ± 0.42 (F6, Anbgl1 and Trcbh2) FPIU/mL.
30657063	5	12	dep	7.69 ± 0.42	1021:1031	arg1	Anbgl1					1038:1043	Anbgl1	1038:1043	Anbgl1	1038:1043	Filter paper activities of T. reesei cellulase were greatly elevated, which were 7.21 ± 0.45 (E7, Aabgl1 and Trcbh2) and 7.69 ± 0.42 (F6, Anbgl1 and Trcbh2) FPIU/mL.
30657063	5	12	dep	7.69 ± 0.42	1021:1031	arg1	F6					1034:1035	F6	1034:1035	F6	1034:1035	Filter paper activities of T. reesei cellulase were greatly elevated, which were 7.21 ± 0.45 (E7, Aabgl1 and Trcbh2) and 7.69 ± 0.42 (F6, Anbgl1 and Trcbh2) FPIU/mL.
30657063	0	13	theme	cellulose	121:129	arg1	capability					141:150	the cellulose degrading capability	117:150	the cellulose degrading capability	117:150	Simultaneous enhancement of the beta-exo synergism and exo-exo synergism in Trichoderma reesei cellulase to increase the cellulose degrading capability.
30657063	3	14	theme	degrading	589:597	arg1	capability					599:608	the cellulose degrading capability	575:608	the cellulose degrading capability	575:608	This work aimed at solving this problem by simultaneous enhancement of the beta-exo synergism and exo-exo synergism in T. reesei cellulase to increase the cellulose degrading capability, i.e. enhanced co-expression of the β-glucosidase gene the cellobiohydrolase II gene of T. reesei.
30657063	11	15	theme	on-site	2020:2026	arg1	production					2038:2047	on-site cellulase production	2020:2047	on-site cellulase production	2020:2047	This strategy also improved the cellulase production, enzymatic hydrolysis yield and the efficiency of the process from SECS to glucose in the context of on-site cellulase production.
30657063	8	16	from	SECS	1358:1361	arg1	release					1345:1351	1 g glucose release	1333:1351	1 g glucose release from SECS	1333:1361	The substrate loading for 1 g glucose release from SECS were decreased, from 2.9637 g SECS using Rut-C30 cellulase to 2.0291 g SECS using E7 cellulase and 2.0573 g SECS using F6 cellulase.
30657063	10	17	theme	Enhanced	1600:1607	arg1	co-expression					1609:1621	CONCLUSIONS Enhanced co-expression	1588:1621	CONCLUSIONS Enhanced co-expression of the β-glucosidase gene and the cellobiohydrolase II gene in T. reesei using the strong promoter Pcbh1 in T. reesei	1588:1739	CONCLUSIONS Enhanced co-expression of the β-glucosidase gene and the cellobiohydrolase II gene in T. reesei using the strong promoter Pcbh1 in T. reesei was proven triumphal in the simultaneous enhancement of the beta-exo synergism and exo-exo synergism in T. reesei cellulase.
30657063	1	18	theme	high	220:223	arg1	costs					236:240	the high production costs	216:240	the high production costs of the lignocellulose-based biorefineries	216:282	BACKGROUND Cellulase is the one of the largest contributors to the high production costs of the lignocellulose-based biorefineries.
30657063	0	19	from	synergism	63:71	arg1	cellulase					95:103	Trichoderma reesei cellulase	76:103	Trichoderma reesei cellulase	76:103	Simultaneous enhancement of the beta-exo synergism and exo-exo synergism in Trichoderma reesei cellulase to increase the cellulose degrading capability.
30657063	11	20	from	production	1908:1917	arg1	SECS					1986:1989	SECS	1986:1989	SECS	1986:1989	This strategy also improved the cellulase production, enzymatic hydrolysis yield and the efficiency of the process from SECS to glucose in the context of on-site cellulase production.
30657063	4	21	theme	gene	761:764	arg1	co-expression					726:738	RESULTS Enhanced co-expression	709:738	RESULTS Enhanced co-expression of the β-glucosidase gene and the cellobiohydrolase II gene in T. reesei using the strong promoter Pcbh1	709:843	RESULTS Enhanced co-expression of the β-glucosidase gene and the cellobiohydrolase II gene in T. reesei using the strong promoter Pcbh1 was found successful in overcoming the two weaknesses.
30657063	3	22	theme	enhanced	616:623	arg1	co-expression					625:637	enhanced co-expression	616:637	enhanced co-expression of the β-glucosidase gene	616:663	This work aimed at solving this problem by simultaneous enhancement of the beta-exo synergism and exo-exo synergism in T. reesei cellulase to increase the cellulose degrading capability, i.e. enhanced co-expression of the β-glucosidase gene the cellobiohydrolase II gene of T. reesei.
30657063	9	23	from	SECS	1544:1547	arg1	result					1501:1506	a result	1499:1506	a result	1499:1506	As a result, the efficiency of the process from SECS to glucose was substantially improved.
30657063	9	23	from	SECS	1544:1547	arg1	efficiency					1513:1522	the efficiency	1509:1522	the efficiency of the process from SECS to glucose	1509:1558	As a result, the efficiency of the process from SECS to glucose was substantially improved.
30657063	9	23	from	SECS	1544:1547	arg1	process					1531:1537	the process	1527:1537	the process from SECS to glucose	1527:1558	As a result, the efficiency of the process from SECS to glucose was substantially improved.
30657063	10	24	theme	synergism	1810:1818	arg1	enhancement					1782:1792	the simultaneous enhancement	1765:1792	the simultaneous enhancement of the beta-exo synergism	1765:1818	CONCLUSIONS Enhanced co-expression of the β-glucosidase gene and the cellobiohydrolase II gene in T. reesei using the strong promoter Pcbh1 in T. reesei was proven triumphal in the simultaneous enhancement of the beta-exo synergism and exo-exo synergism in T. reesei cellulase.
30657063	10	24	theme	synergism	1810:1818	arg1	synergism					1832:1840	exo-exo synergism	1824:1840	exo-exo synergism in T. reesei cellulase	1824:1863	CONCLUSIONS Enhanced co-expression of the β-glucosidase gene and the cellobiohydrolase II gene in T. reesei using the strong promoter Pcbh1 in T. reesei was proven triumphal in the simultaneous enhancement of the beta-exo synergism and exo-exo synergism in T. reesei cellulase.
30657063	8	25	theme	2.0573 g	1462:1469	arg1	SECS					1471:1474	2.0573 g SECS	1462:1474	2.0573 g SECS using F6 cellulase	1462:1493	The substrate loading for 1 g glucose release from SECS were decreased, from 2.9637 g SECS using Rut-C30 cellulase to 2.0291 g SECS using E7 cellulase and 2.0573 g SECS using F6 cellulase.
30657063	7	26	theme	cellulase	1229:1237	arg1	%					1254:1254	Rut-C30 cellulase to 87.98 ± 0.65%	1221:1254	Rut-C30 cellulase to 87.98 ± 0.65% by E7 cellulase	1221:1270	Enzymatic hydrolysis yields were also improved, from 67.22 ± 1.61% by Rut-C30 cellulase to 87.98 ± 0.65% by E7 cellulase and 86.50 ± 1.01% by F6 cellulase.
30657063	3	27	theme	β-glucosidase	646:658	arg1	gene					660:663	the β-glucosidase gene	642:663	the β-glucosidase gene	642:663	This work aimed at solving this problem by simultaneous enhancement of the beta-exo synergism and exo-exo synergism in T. reesei cellulase to increase the cellulose degrading capability, i.e. enhanced co-expression of the β-glucosidase gene the cellobiohydrolase II gene of T. reesei.
30657063	5	28	theme	reesei	930:935	arg1	cellulase					937:945	T. reesei cellulase	927:945	T. reesei cellulase	927:945	Filter paper activities of T. reesei cellulase were greatly elevated, which were 7.21 ± 0.45 (E7, Aabgl1 and Trcbh2) and 7.69 ± 0.42 (F6, Anbgl1 and Trcbh2) FPIU/mL.
30657063	0	29	theme	Trichoderma	76:86	arg1	cellulase					95:103	Trichoderma reesei cellulase	76:103	Trichoderma reesei cellulase	76:103	Simultaneous enhancement of the beta-exo synergism and exo-exo synergism in Trichoderma reesei cellulase to increase the cellulose degrading capability.
30657063	11	30	theme	process	1973:1979	arg1	production					1908:1917	the cellulase production	1894:1917	the cellulase production	1894:1917	This strategy also improved the cellulase production, enzymatic hydrolysis yield and the efficiency of the process from SECS to glucose in the context of on-site cellulase production.
30657063	11	30	theme	process	1973:1979	arg1	yield					1941:1945	enzymatic hydrolysis yield	1920:1945	enzymatic hydrolysis yield	1920:1945	This strategy also improved the cellulase production, enzymatic hydrolysis yield and the efficiency of the process from SECS to glucose in the context of on-site cellulase production.
30657063	11	30	theme	process	1973:1979	arg1	efficiency					1955:1964	the efficiency	1951:1964	the efficiency of the process from SECS to glucose	1951:2000	This strategy also improved the cellulase production, enzymatic hydrolysis yield and the efficiency of the process from SECS to glucose in the context of on-site cellulase production.
30657063	8	31	theme	1 g	1333:1335	arg1	release					1345:1351	1 g glucose release	1333:1351	1 g glucose release from SECS	1333:1361	The substrate loading for 1 g glucose release from SECS were decreased, from 2.9637 g SECS using Rut-C30 cellulase to 2.0291 g SECS using E7 cellulase and 2.0573 g SECS using F6 cellulase.
30657063	3	32	dep	synergism	508:516	arg1	the					495:497	the	495:497	the	495:497	This work aimed at solving this problem by simultaneous enhancement of the beta-exo synergism and exo-exo synergism in T. reesei cellulase to increase the cellulose degrading capability, i.e. enhanced co-expression of the β-glucosidase gene the cellobiohydrolase II gene of T. reesei.
30657063	11	33	theme	enzymatic	1920:1928	arg1	yield					1941:1945	enzymatic hydrolysis yield	1920:1945	enzymatic hydrolysis yield	1920:1945	This strategy also improved the cellulase production, enzymatic hydrolysis yield and the efficiency of the process from SECS to glucose in the context of on-site cellulase production.
30657063	10	34	theme	gene	1644:1647	arg1	co-expression					1609:1621	CONCLUSIONS Enhanced co-expression	1588:1621	CONCLUSIONS Enhanced co-expression of the β-glucosidase gene and the cellobiohydrolase II gene in T. reesei using the strong promoter Pcbh1 in T. reesei	1588:1739	CONCLUSIONS Enhanced co-expression of the β-glucosidase gene and the cellobiohydrolase II gene in T. reesei using the strong promoter Pcbh1 in T. reesei was proven triumphal in the simultaneous enhancement of the beta-exo synergism and exo-exo synergism in T. reesei cellulase.
30657063	2	35	theme	cellobiohydrolase	402:418	arg1	II					420:421	cellobiohydrolase II	402:421	cellobiohydrolase II	402:421	As the most widely used cellulase producer, Trichoderma reesei has two weaknesses, deficiencies in β-glucosidase and cellobiohydrolase II.
30657063	0	36	theme	Simultaneous	0:11	arg1	enhancement					13:23	Simultaneous enhancement	0:23	Simultaneous enhancement of the beta-exo synergism	0:49	Simultaneous enhancement of the beta-exo synergism and exo-exo synergism in Trichoderma reesei cellulase to increase the cellulose degrading capability.
30657063	12	37	from	attempt	2077:2083	arg1	optimization					2114:2125	the cellulase composition optimization	2088:2125	the cellulase composition optimization	2088:2125	This work is a commendable attempt in the cellulase composition optimization at the transcriptional level.
30657063	10	38	theme	promoter	1713:1720	arg1	Pcbh1					1722:1726	the strong promoter Pcbh1	1702:1726	the strong promoter Pcbh1 in T. reesei	1702:1739	CONCLUSIONS Enhanced co-expression of the β-glucosidase gene and the cellobiohydrolase II gene in T. reesei using the strong promoter Pcbh1 in T. reesei was proven triumphal in the simultaneous enhancement of the beta-exo synergism and exo-exo synergism in T. reesei cellulase.
30657063	1	39	theme	BACKGROUND	153:162	arg1	Cellulase					164:172	BACKGROUND Cellulase	153:172	BACKGROUND Cellulase	153:172	BACKGROUND Cellulase is the one of the largest contributors to the high production costs of the lignocellulose-based biorefineries.
30657063	1	39	theme	BACKGROUND	153:162	arg1	one					181:183	one	181:183	one	181:183	BACKGROUND Cellulase is the one of the largest contributors to the high production costs of the lignocellulose-based biorefineries.
30657063	1	39	theme	BACKGROUND	153:162	arg1	contributors					200:211	the largest contributors	188:211	the largest contributors to the high production costs of the lignocellulose-based biorefineries	188:282	BACKGROUND Cellulase is the one of the largest contributors to the high production costs of the lignocellulose-based biorefineries.
30657063	11	40	theme	cellulase	1898:1906	arg1	production					1908:1917	the cellulase production	1894:1917	the cellulase production	1894:1917	This strategy also improved the cellulase production, enzymatic hydrolysis yield and the efficiency of the process from SECS to glucose in the context of on-site cellulase production.
30657063	3	41	theme	simultaneous	467:478	arg1	enhancement					480:490	simultaneous enhancement	467:490	simultaneous enhancement of the beta-exo synergism and exo-exo synergism in T. reesei cellulase to increase the cellulose degrading capability, i.e. enhanced co-expression of the β-glucosidase gene the cellobiohydrolase II gene of T. reesei	467:706	This work aimed at solving this problem by simultaneous enhancement of the beta-exo synergism and exo-exo synergism in T. reesei cellulase to increase the cellulose degrading capability, i.e. enhanced co-expression of the β-glucosidase gene the cellobiohydrolase II gene of T. reesei.
30657063	0	42	theme	beta-exo	32:39	arg1	synergism					41:49	the beta-exo synergism	28:49	the beta-exo synergism	28:49	Simultaneous enhancement of the beta-exo synergism and exo-exo synergism in Trichoderma reesei cellulase to increase the cellulose degrading capability.
30657063	7	43	theme	Enzymatic	1151:1159	arg1	yields					1172:1177	Enzymatic hydrolysis yields	1151:1177	Enzymatic hydrolysis yields	1151:1177	Enzymatic hydrolysis yields were also improved, from 67.22 ± 1.61% by Rut-C30 cellulase to 87.98 ± 0.65% by E7 cellulase and 86.50 ± 1.01% by F6 cellulase.
30657063	12	44	theme	transcriptional	2134:2148	arg1	level					2150:2154	the transcriptional level	2130:2154	the transcriptional level	2130:2154	This work is a commendable attempt in the cellulase composition optimization at the transcriptional level.
30657063	10	45	theme	exo-exo	1824:1830	arg1	synergism					1832:1840	exo-exo synergism	1824:1840	exo-exo synergism in T. reesei cellulase	1824:1863	CONCLUSIONS Enhanced co-expression of the β-glucosidase gene and the cellobiohydrolase II gene in T. reesei using the strong promoter Pcbh1 in T. reesei was proven triumphal in the simultaneous enhancement of the beta-exo synergism and exo-exo synergism in T. reesei cellulase.
30657063	3	46	from	enhancement	480:490	arg1	cellulase					553:561	T. reesei cellulase	543:561	T. reesei cellulase	543:561	This work aimed at solving this problem by simultaneous enhancement of the beta-exo synergism and exo-exo synergism in T. reesei cellulase to increase the cellulose degrading capability, i.e. enhanced co-expression of the β-glucosidase gene the cellobiohydrolase II gene of T. reesei.
30657063	10	47	from	gene	1644:1647	arg1	reesei					1689:1694	T. reesei	1686:1694	T. reesei using the strong promoter Pcbh1 in T. reesei	1686:1739	CONCLUSIONS Enhanced co-expression of the β-glucosidase gene and the cellobiohydrolase II gene in T. reesei using the strong promoter Pcbh1 in T. reesei was proven triumphal in the simultaneous enhancement of the beta-exo synergism and exo-exo synergism in T. reesei cellulase.
30657063	8	48	theme	Rut-C30	1404:1410	arg1	cellulase					1412:1420	Rut-C30 cellulase	1404:1420	Rut-C30 cellulase to 2.0291 g SECS using E7 cellulase and 2.0573 g SECS using F6 cellulase	1404:1493	The substrate loading for 1 g glucose release from SECS were decreased, from 2.9637 g SECS using Rut-C30 cellulase to 2.0291 g SECS using E7 cellulase and 2.0573 g SECS using F6 cellulase.
30657063	3	49	theme	beta-exo	499:506	arg1	synergism					508:516	beta-exo synergism	499:516	beta-exo synergism	499:516	This work aimed at solving this problem by simultaneous enhancement of the beta-exo synergism and exo-exo synergism in T. reesei cellulase to increase the cellulose degrading capability, i.e. enhanced co-expression of the β-glucosidase gene the cellobiohydrolase II gene of T. reesei.
30657063	1	50	theme	production	225:234	arg1	costs					236:240	the high production costs	216:240	the high production costs of the lignocellulose-based biorefineries	216:282	BACKGROUND Cellulase is the one of the largest contributors to the high production costs of the lignocellulose-based biorefineries.
30657063	6	51	theme	parental	1105:1112	arg1	Rut-C30					1121:1127	the parental strain Rut-C30	1101:1127	the parental strain Rut-C30	1101:1127	They were much higher than that of the parental strain Rut-C30, 2.45 ± 0.36 FPIU/mL.
30657063	6	51	theme	parental	1105:1112	arg1	FPIU/mL					1142:1148	2.45 ± 0.36 FPIU/mL	1130:1148	2.45 ± 0.36 FPIU/mL	1130:1148	They were much higher than that of the parental strain Rut-C30, 2.45 ± 0.36 FPIU/mL.
30657063	10	52	theme	reesei	1848:1853	arg1	cellulase					1855:1863	T. reesei cellulase	1845:1863	T. reesei cellulase	1845:1863	CONCLUSIONS Enhanced co-expression of the β-glucosidase gene and the cellobiohydrolase II gene in T. reesei using the strong promoter Pcbh1 in T. reesei was proven triumphal in the simultaneous enhancement of the beta-exo synergism and exo-exo synergism in T. reesei cellulase.
30657063	3	53	theme	T.	543:544	arg1	cellulase					553:561	T. reesei cellulase	543:561	T. reesei cellulase	543:561	This work aimed at solving this problem by simultaneous enhancement of the beta-exo synergism and exo-exo synergism in T. reesei cellulase to increase the cellulose degrading capability, i.e. enhanced co-expression of the β-glucosidase gene the cellobiohydrolase II gene of T. reesei.
30657063	6	54	theme	2.45 ± 0.36	1130:1140	arg1	Rut-C30					1121:1127	the parental strain Rut-C30	1101:1127	the parental strain Rut-C30	1101:1127	They were much higher than that of the parental strain Rut-C30, 2.45 ± 0.36 FPIU/mL.
30657063	6	54	theme	2.45 ± 0.36	1130:1140	arg1	FPIU/mL					1142:1148	2.45 ± 0.36 FPIU/mL	1130:1148	2.45 ± 0.36 FPIU/mL	1130:1148	They were much higher than that of the parental strain Rut-C30, 2.45 ± 0.36 FPIU/mL.
30657063	3	55	theme	synergism	530:538	arg1	enhancement					480:490	simultaneous enhancement	467:490	simultaneous enhancement of the beta-exo synergism and exo-exo synergism in T. reesei cellulase to increase the cellulose degrading capability, i.e. enhanced co-expression of the β-glucosidase gene the cellobiohydrolase II gene of T. reesei	467:706	This work aimed at solving this problem by simultaneous enhancement of the beta-exo synergism and exo-exo synergism in T. reesei cellulase to increase the cellulose degrading capability, i.e. enhanced co-expression of the β-glucosidase gene the cellobiohydrolase II gene of T. reesei.
30657063	12	56	from	level	2150:2154	arg1	work					2055:2058	This work	2050:2058	This work	2050:2058	This work is a commendable attempt in the cellulase composition optimization at the transcriptional level.
30657063	12	56	from	level	2150:2154	arg1	attempt					2077:2083	a commendable attempt	2063:2083	a commendable attempt in the cellulase composition optimization at the transcriptional level	2063:2154	This work is a commendable attempt in the cellulase composition optimization at the transcriptional level.
30657063	1	57	theme	lignocellulose-based	249:268	arg1	biorefineries					270:282	the lignocellulose-based biorefineries	245:282	the lignocellulose-based biorefineries	245:282	BACKGROUND Cellulase is the one of the largest contributors to the high production costs of the lignocellulose-based biorefineries.
30657063	2	58	theme	used	304:307	arg1	producer					319:326	the most widely used cellulase producer	288:326	the most widely used cellulase producer	288:326	As the most widely used cellulase producer, Trichoderma reesei has two weaknesses, deficiencies in β-glucosidase and cellobiohydrolase II.
30657063	2	58	theme	used	304:307	arg1	weaknesses					356:365	two weaknesses	352:365	two weaknesses	352:365	As the most widely used cellulase producer, Trichoderma reesei has two weaknesses, deficiencies in β-glucosidase and cellobiohydrolase II.
30657063	10	59	from	reesei	1689:1694	arg1	co-expression					1609:1621	CONCLUSIONS Enhanced co-expression	1588:1621	CONCLUSIONS Enhanced co-expression of the β-glucosidase gene and the cellobiohydrolase II gene in T. reesei using the strong promoter Pcbh1 in T. reesei	1588:1739	CONCLUSIONS Enhanced co-expression of the β-glucosidase gene and the cellobiohydrolase II gene in T. reesei using the strong promoter Pcbh1 in T. reesei was proven triumphal in the simultaneous enhancement of the beta-exo synergism and exo-exo synergism in T. reesei cellulase.
30657063	11	60	from	efficiency	1955:1964	arg1	SECS					1986:1989	SECS	1986:1989	SECS	1986:1989	This strategy also improved the cellulase production, enzymatic hydrolysis yield and the efficiency of the process from SECS to glucose in the context of on-site cellulase production.
30657063	5	61	theme	Filter	900:905	arg1	activities					913:922	Filter paper activities	900:922	Filter paper activities of T. reesei cellulase	900:945	Filter paper activities of T. reesei cellulase were greatly elevated, which were 7.21 ± 0.45 (E7, Aabgl1 and Trcbh2) and 7.69 ± 0.42 (F6, Anbgl1 and Trcbh2) FPIU/mL.
30657063	10	62	from	co-expression	1609:1621	arg1	reesei					1689:1694	T. reesei	1686:1694	T. reesei using the strong promoter Pcbh1 in T. reesei	1686:1739	CONCLUSIONS Enhanced co-expression of the β-glucosidase gene and the cellobiohydrolase II gene in T. reesei using the strong promoter Pcbh1 in T. reesei was proven triumphal in the simultaneous enhancement of the beta-exo synergism and exo-exo synergism in T. reesei cellulase.
30657063	2	63	from	deficiencies	368:379	arg1	II					420:421	cellobiohydrolase II	402:421	cellobiohydrolase II	402:421	As the most widely used cellulase producer, Trichoderma reesei has two weaknesses, deficiencies in β-glucosidase and cellobiohydrolase II.
30657063	2	63	from	deficiencies	368:379	arg1	β-glucosidase					384:396	β-glucosidase	384:396	β-glucosidase	384:396	As the most widely used cellulase producer, Trichoderma reesei has two weaknesses, deficiencies in β-glucosidase and cellobiohydrolase II.
30657063	12	64	theme	cellulase	2092:2100	arg1	optimization					2114:2125	the cellulase composition optimization	2088:2125	the cellulase composition optimization	2088:2125	This work is a commendable attempt in the cellulase composition optimization at the transcriptional level.
30657063	3	65	from	synergism	508:516	arg1	cellulase					553:561	T. reesei cellulase	543:561	T. reesei cellulase	543:561	This work aimed at solving this problem by simultaneous enhancement of the beta-exo synergism and exo-exo synergism in T. reesei cellulase to increase the cellulose degrading capability, i.e. enhanced co-expression of the β-glucosidase gene the cellobiohydrolase II gene of T. reesei.
30657063	2	66	theme	Trichoderma	329:339	arg1	reesei					341:346	Trichoderma reesei	329:346	Trichoderma reesei	329:346	As the most widely used cellulase producer, Trichoderma reesei has two weaknesses, deficiencies in β-glucosidase and cellobiohydrolase II.
30657063	3	67	dep	co-expression	625:637	arg1	gene					690:693	the cellobiohydrolase II gene	665:693	the cellobiohydrolase II gene of T. reesei	665:706	This work aimed at solving this problem by simultaneous enhancement of the beta-exo synergism and exo-exo synergism in T. reesei cellulase to increase the cellulose degrading capability, i.e. enhanced co-expression of the β-glucosidase gene the cellobiohydrolase II gene of T. reesei.
30657063	3	67	dep	co-expression	625:637	arg1	i.e.					611:614	i.e.	611:614	i.e.	611:614	This work aimed at solving this problem by simultaneous enhancement of the beta-exo synergism and exo-exo synergism in T. reesei cellulase to increase the cellulose degrading capability, i.e. enhanced co-expression of the β-glucosidase gene the cellobiohydrolase II gene of T. reesei.
30657063	0	68	theme	degrading	131:139	arg1	capability					141:150	the cellulose degrading capability	117:150	the cellulose degrading capability	117:150	Simultaneous enhancement of the beta-exo synergism and exo-exo synergism in Trichoderma reesei cellulase to increase the cellulose degrading capability.
30657063	3	69	theme	cellulose	579:587	arg1	capability					599:608	the cellulose degrading capability	575:608	the cellulose degrading capability	575:608	This work aimed at solving this problem by simultaneous enhancement of the beta-exo synergism and exo-exo synergism in T. reesei cellulase to increase the cellulose degrading capability, i.e. enhanced co-expression of the β-glucosidase gene the cellobiohydrolase II gene of T. reesei.
30657063	1	70	theme	contributors	200:211	arg1	Cellulase					164:172	BACKGROUND Cellulase	153:172	BACKGROUND Cellulase	153:172	BACKGROUND Cellulase is the one of the largest contributors to the high production costs of the lignocellulose-based biorefineries.
30657063	1	70	theme	contributors	200:211	arg1	one					181:183	one	181:183	one	181:183	BACKGROUND Cellulase is the one of the largest contributors to the high production costs of the lignocellulose-based biorefineries.
30657063	1	70	theme	contributors	200:211	arg1	contributors					200:211	the largest contributors	188:211	the largest contributors to the high production costs of the lignocellulose-based biorefineries	188:282	BACKGROUND Cellulase is the one of the largest contributors to the high production costs of the lignocellulose-based biorefineries.
30657063	8	71	theme	substrate	1311:1319	arg1	loading					1321:1327	The substrate loading	1307:1327	The substrate loading for 1 g glucose release from SECS	1307:1361	The substrate loading for 1 g glucose release from SECS were decreased, from 2.9637 g SECS using Rut-C30 cellulase to 2.0291 g SECS using E7 cellulase and 2.0573 g SECS using F6 cellulase.
30657063	12	72	theme	commendable	2065:2075	arg1	work					2055:2058	This work	2050:2058	This work	2050:2058	This work is a commendable attempt in the cellulase composition optimization at the transcriptional level.
30657063	12	72	theme	commendable	2065:2075	arg1	attempt					2077:2083	a commendable attempt	2063:2083	a commendable attempt in the cellulase composition optimization at the transcriptional level	2063:2154	This work is a commendable attempt in the cellulase composition optimization at the transcriptional level.
30657063	11	73	theme	cellulase	2028:2036	arg1	production					2038:2047	on-site cellulase production	2020:2047	on-site cellulase production	2020:2047	This strategy also improved the cellulase production, enzymatic hydrolysis yield and the efficiency of the process from SECS to glucose in the context of on-site cellulase production.
30657063	8	74	theme	E7	1445:1446	arg1	cellulase					1448:1456	E7 cellulase	1445:1456	E7 cellulase	1445:1456	The substrate loading for 1 g glucose release from SECS were decreased, from 2.9637 g SECS using Rut-C30 cellulase to 2.0291 g SECS using E7 cellulase and 2.0573 g SECS using F6 cellulase.
30657063	10	75	theme	CONCLUSIONS	1588:1598	arg1	co-expression					1609:1621	CONCLUSIONS Enhanced co-expression	1588:1621	CONCLUSIONS Enhanced co-expression of the β-glucosidase gene and the cellobiohydrolase II gene in T. reesei using the strong promoter Pcbh1 in T. reesei	1588:1739	CONCLUSIONS Enhanced co-expression of the β-glucosidase gene and the cellobiohydrolase II gene in T. reesei using the strong promoter Pcbh1 in T. reesei was proven triumphal in the simultaneous enhancement of the beta-exo synergism and exo-exo synergism in T. reesei cellulase.
30657063	10	76	theme	simultaneous	1769:1780	arg1	enhancement					1782:1792	the simultaneous enhancement	1765:1792	the simultaneous enhancement of the beta-exo synergism	1765:1818	CONCLUSIONS Enhanced co-expression of the β-glucosidase gene and the cellobiohydrolase II gene in T. reesei using the strong promoter Pcbh1 in T. reesei was proven triumphal in the simultaneous enhancement of the beta-exo synergism and exo-exo synergism in T. reesei cellulase.
30657063	10	77	theme	strong	1706:1711	arg1	Pcbh1					1722:1726	the strong promoter Pcbh1	1702:1726	the strong promoter Pcbh1 in T. reesei	1702:1739	CONCLUSIONS Enhanced co-expression of the β-glucosidase gene and the cellobiohydrolase II gene in T. reesei using the strong promoter Pcbh1 in T. reesei was proven triumphal in the simultaneous enhancement of the beta-exo synergism and exo-exo synergism in T. reesei cellulase.
30657063	4	78	theme	β-glucosidase	747:759	arg1	gene					761:764	the β-glucosidase gene	743:764	the β-glucosidase gene	743:764	RESULTS Enhanced co-expression of the β-glucosidase gene and the cellobiohydrolase II gene in T. reesei using the strong promoter Pcbh1 was found successful in overcoming the two weaknesses.
30657063	4	79	theme	promoter	830:837	arg1	Pcbh1					839:843	the strong promoter Pcbh1	819:843	the strong promoter Pcbh1	819:843	RESULTS Enhanced co-expression of the β-glucosidase gene and the cellobiohydrolase II gene in T. reesei using the strong promoter Pcbh1 was found successful in overcoming the two weaknesses.
30657063	3	80	theme	gene	660:663	arg1	co-expression					625:637	enhanced co-expression	616:637	enhanced co-expression of the β-glucosidase gene	616:663	This work aimed at solving this problem by simultaneous enhancement of the beta-exo synergism and exo-exo synergism in T. reesei cellulase to increase the cellulose degrading capability, i.e. enhanced co-expression of the β-glucosidase gene the cellobiohydrolase II gene of T. reesei.
30657063	10	81	from	Pcbh1	1722:1726	arg1	reesei					1734:1739	T. reesei	1731:1739	T. reesei	1731:1739	CONCLUSIONS Enhanced co-expression of the β-glucosidase gene and the cellobiohydrolase II gene in T. reesei using the strong promoter Pcbh1 in T. reesei was proven triumphal in the simultaneous enhancement of the beta-exo synergism and exo-exo synergism in T. reesei cellulase.
30657063	6	82	theme	strain	1114:1119	arg1	Rut-C30					1121:1127	the parental strain Rut-C30	1101:1127	the parental strain Rut-C30	1101:1127	They were much higher than that of the parental strain Rut-C30, 2.45 ± 0.36 FPIU/mL.
30657063	6	82	theme	strain	1114:1119	arg1	FPIU/mL					1142:1148	2.45 ± 0.36 FPIU/mL	1130:1148	2.45 ± 0.36 FPIU/mL	1130:1148	They were much higher than that of the parental strain Rut-C30, 2.45 ± 0.36 FPIU/mL.
30657063	10	83	from	triumphal	1752:1760	arg1	enhancement					1782:1792	the simultaneous enhancement	1765:1792	the simultaneous enhancement of the beta-exo synergism	1765:1818	CONCLUSIONS Enhanced co-expression of the β-glucosidase gene and the cellobiohydrolase II gene in T. reesei using the strong promoter Pcbh1 in T. reesei was proven triumphal in the simultaneous enhancement of the beta-exo synergism and exo-exo synergism in T. reesei cellulase.
30657063	10	83	from	triumphal	1752:1760	arg1	synergism					1832:1840	exo-exo synergism	1824:1840	exo-exo synergism in T. reesei cellulase	1824:1863	CONCLUSIONS Enhanced co-expression of the β-glucosidase gene and the cellobiohydrolase II gene in T. reesei using the strong promoter Pcbh1 in T. reesei was proven triumphal in the simultaneous enhancement of the beta-exo synergism and exo-exo synergism in T. reesei cellulase.
30657063	10	84	theme	beta-exo	1801:1808	arg1	synergism					1810:1818	the beta-exo synergism	1797:1818	the beta-exo synergism	1797:1818	CONCLUSIONS Enhanced co-expression of the β-glucosidase gene and the cellobiohydrolase II gene in T. reesei using the strong promoter Pcbh1 in T. reesei was proven triumphal in the simultaneous enhancement of the beta-exo synergism and exo-exo synergism in T. reesei cellulase.
30657063	5	85	theme	T.	927:928	arg1	cellulase					937:945	T. reesei cellulase	927:945	T. reesei cellulase	927:945	Filter paper activities of T. reesei cellulase were greatly elevated, which were 7.21 ± 0.45 (E7, Aabgl1 and Trcbh2) and 7.69 ± 0.42 (F6, Anbgl1 and Trcbh2) FPIU/mL.
30657063	8	86	theme	F6	1482:1483	arg1	cellulase					1485:1493	F6 cellulase	1482:1493	F6 cellulase	1482:1493	The substrate loading for 1 g glucose release from SECS were decreased, from 2.9637 g SECS using Rut-C30 cellulase to 2.0291 g SECS using E7 cellulase and 2.0573 g SECS using F6 cellulase.
30657063	4	87	theme	Enhanced	717:724	arg1	co-expression					726:738	RESULTS Enhanced co-expression	709:738	RESULTS Enhanced co-expression of the β-glucosidase gene and the cellobiohydrolase II gene in T. reesei using the strong promoter Pcbh1	709:843	RESULTS Enhanced co-expression of the β-glucosidase gene and the cellobiohydrolase II gene in T. reesei using the strong promoter Pcbh1 was found successful in overcoming the two weaknesses.
30657063	3	88	from	cellulase	553:561	arg1	enhancement					480:490	simultaneous enhancement	467:490	simultaneous enhancement of the beta-exo synergism and exo-exo synergism in T. reesei cellulase to increase the cellulose degrading capability, i.e. enhanced co-expression of the β-glucosidase gene the cellobiohydrolase II gene of T. reesei	467:706	This work aimed at solving this problem by simultaneous enhancement of the beta-exo synergism and exo-exo synergism in T. reesei cellulase to increase the cellulose degrading capability, i.e. enhanced co-expression of the β-glucosidase gene the cellobiohydrolase II gene of T. reesei.
30657063	0	89	theme	reesei	88:93	arg1	cellulase					95:103	Trichoderma reesei cellulase	76:103	Trichoderma reesei cellulase	76:103	Simultaneous enhancement of the beta-exo synergism and exo-exo synergism in Trichoderma reesei cellulase to increase the cellulose degrading capability.
30657063	2	90	contain	has	348:350	arg1	reesei					341:346	Trichoderma reesei	329:346	Trichoderma reesei	329:346	As the most widely used cellulase producer, Trichoderma reesei has two weaknesses, deficiencies in β-glucosidase and cellobiohydrolase II.
30657063	2	90	contain	has	348:350	arg2	weaknesses					356:365	two weaknesses	352:365	two weaknesses	352:365	As the most widely used cellulase producer, Trichoderma reesei has two weaknesses, deficiencies in β-glucosidase and cellobiohydrolase II.
30657063	2	90	contain	has	348:350	arg2	producer					319:326	the most widely used cellulase producer	288:326	the most widely used cellulase producer	288:326	As the most widely used cellulase producer, Trichoderma reesei has two weaknesses, deficiencies in β-glucosidase and cellobiohydrolase II.
30657063	2	90	contain	has	348:350	arg2	deficiencies					368:379	deficiencies	368:379	deficiencies in β-glucosidase and cellobiohydrolase II	368:421	As the most widely used cellulase producer, Trichoderma reesei has two weaknesses, deficiencies in β-glucosidase and cellobiohydrolase II.
30657063	5	91	theme	cellulase	937:945	arg1	activities					913:922	Filter paper activities	900:922	Filter paper activities of T. reesei cellulase	900:945	Filter paper activities of T. reesei cellulase were greatly elevated, which were 7.21 ± 0.45 (E7, Aabgl1 and Trcbh2) and 7.69 ± 0.42 (F6, Anbgl1 and Trcbh2) FPIU/mL.
30657063	0	92	from	enhancement	13:23	arg1	cellulase					95:103	Trichoderma reesei cellulase	76:103	Trichoderma reesei cellulase	76:103	Simultaneous enhancement of the beta-exo synergism and exo-exo synergism in Trichoderma reesei cellulase to increase the cellulose degrading capability.
30657063	5	93	dep	7.21 ± 0.45	981:991	arg1	FPIU/mL					1057:1063	FPIU/mL	1057:1063	FPIU/mL	1057:1063	Filter paper activities of T. reesei cellulase were greatly elevated, which were 7.21 ± 0.45 (E7, Aabgl1 and Trcbh2) and 7.69 ± 0.42 (F6, Anbgl1 and Trcbh2) FPIU/mL.
30657063	5	93	dep	7.21 ± 0.45	981:991	arg1	Aabgl1					998:1003	Aabgl1	998:1003	Aabgl1	998:1003	Filter paper activities of T. reesei cellulase were greatly elevated, which were 7.21 ± 0.45 (E7, Aabgl1 and Trcbh2) and 7.69 ± 0.42 (F6, Anbgl1 and Trcbh2) FPIU/mL.
30657063	5	93	dep	7.21 ± 0.45	981:991	arg1	E7					994:995	E7	994:995	E7	994:995	Filter paper activities of T. reesei cellulase were greatly elevated, which were 7.21 ± 0.45 (E7, Aabgl1 and Trcbh2) and 7.69 ± 0.42 (F6, Anbgl1 and Trcbh2) FPIU/mL.
30657063	5	93	dep	7.21 ± 0.45	981:991	arg1	Trcbh2					1009:1014	Trcbh2	1009:1014	Trcbh2	1009:1014	Filter paper activities of T. reesei cellulase were greatly elevated, which were 7.21 ± 0.45 (E7, Aabgl1 and Trcbh2) and 7.69 ± 0.42 (F6, Anbgl1 and Trcbh2) FPIU/mL.
30657063	9	94	from	efficiency	1513:1522	arg1	SECS					1544:1547	SECS	1544:1547	SECS	1544:1547	As a result, the efficiency of the process from SECS to glucose was substantially improved.
30657063	10	95	theme	gene	1678:1681	arg1	co-expression					1609:1621	CONCLUSIONS Enhanced co-expression	1588:1621	CONCLUSIONS Enhanced co-expression of the β-glucosidase gene and the cellobiohydrolase II gene in T. reesei using the strong promoter Pcbh1 in T. reesei	1588:1739	CONCLUSIONS Enhanced co-expression of the β-glucosidase gene and the cellobiohydrolase II gene in T. reesei using the strong promoter Pcbh1 in T. reesei was proven triumphal in the simultaneous enhancement of the beta-exo synergism and exo-exo synergism in T. reesei cellulase.
30657063	8	96	theme	glucose	1337:1343	arg1	release					1345:1351	1 g glucose release	1333:1351	1 g glucose release from SECS	1333:1361	The substrate loading for 1 g glucose release from SECS were decreased, from 2.9637 g SECS using Rut-C30 cellulase to 2.0291 g SECS using E7 cellulase and 2.0573 g SECS using F6 cellulase.
30657063	10	97	theme	β-glucosidase	1630:1642	arg1	gene					1644:1647	the β-glucosidase gene	1626:1647	the β-glucosidase gene	1626:1647	CONCLUSIONS Enhanced co-expression of the β-glucosidase gene and the cellobiohydrolase II gene in T. reesei using the strong promoter Pcbh1 in T. reesei was proven triumphal in the simultaneous enhancement of the beta-exo synergism and exo-exo synergism in T. reesei cellulase.
30657063	9	98	theme	process	1531:1537	arg1	result					1501:1506	a result	1499:1506	a result	1499:1506	As a result, the efficiency of the process from SECS to glucose was substantially improved.
30657063	9	98	theme	process	1531:1537	arg1	efficiency					1513:1522	the efficiency	1509:1522	the efficiency of the process from SECS to glucose	1509:1558	As a result, the efficiency of the process from SECS to glucose was substantially improved.
30657063	10	99	from	enhancement	1782:1792	arg1	cellulase					1855:1863	T. reesei cellulase	1845:1863	T. reesei cellulase	1845:1863	CONCLUSIONS Enhanced co-expression of the β-glucosidase gene and the cellobiohydrolase II gene in T. reesei using the strong promoter Pcbh1 in T. reesei was proven triumphal in the simultaneous enhancement of the beta-exo synergism and exo-exo synergism in T. reesei cellulase.
30657063	10	99	from	enhancement	1782:1792	arg1	triumphal					1752:1760	triumphal	1752:1760	triumphal	1752:1760	CONCLUSIONS Enhanced co-expression of the β-glucosidase gene and the cellobiohydrolase II gene in T. reesei using the strong promoter Pcbh1 in T. reesei was proven triumphal in the simultaneous enhancement of the beta-exo synergism and exo-exo synergism in T. reesei cellulase.
30657063	3	100	theme	reesei	701:706	arg1	gene					690:693	the cellobiohydrolase II gene	665:693	the cellobiohydrolase II gene of T. reesei	665:706	This work aimed at solving this problem by simultaneous enhancement of the beta-exo synergism and exo-exo synergism in T. reesei cellulase to increase the cellulose degrading capability, i.e. enhanced co-expression of the β-glucosidase gene the cellobiohydrolase II gene of T. reesei.
30657063	4	101	theme	cellobiohydrolase	774:790	arg1	gene					795:798	the cellobiohydrolase II gene	770:798	the cellobiohydrolase II gene	770:798	RESULTS Enhanced co-expression of the β-glucosidase gene and the cellobiohydrolase II gene in T. reesei using the strong promoter Pcbh1 was found successful in overcoming the two weaknesses.
30657063	8	102	theme	2.0291 g	1425:1432	arg1	SECS					1434:1437	2.0291 g SECS	1425:1437	2.0291 g SECS using E7 cellulase	1425:1456	The substrate loading for 1 g glucose release from SECS were decreased, from 2.9637 g SECS using Rut-C30 cellulase to 2.0291 g SECS using E7 cellulase and 2.0573 g SECS using F6 cellulase.
30657063	7	103	theme	hydrolysis	1161:1170	arg1	yields					1172:1177	Enzymatic hydrolysis yields	1151:1177	Enzymatic hydrolysis yields	1151:1177	Enzymatic hydrolysis yields were also improved, from 67.22 ± 1.61% by Rut-C30 cellulase to 87.98 ± 0.65% by E7 cellulase and 86.50 ± 1.01% by F6 cellulase.
30657063	7	104	dep	%	1254:1254	arg1	to					1239:1240	to	1239:1240	to	1239:1240	Enzymatic hydrolysis yields were also improved, from 67.22 ± 1.61% by Rut-C30 cellulase to 87.98 ± 0.65% by E7 cellulase and 86.50 ± 1.01% by F6 cellulase.
30657063	7	105	theme	F6	1293:1294	arg1	cellulase					1296:1304	F6 cellulase	1293:1304	F6 cellulase	1293:1304	Enzymatic hydrolysis yields were also improved, from 67.22 ± 1.61% by Rut-C30 cellulase to 87.98 ± 0.65% by E7 cellulase and 86.50 ± 1.01% by F6 cellulase.
30657063	10	106	theme	T.	1845:1846	arg1	cellulase					1855:1863	T. reesei cellulase	1845:1863	T. reesei cellulase	1845:1863	CONCLUSIONS Enhanced co-expression of the β-glucosidase gene and the cellobiohydrolase II gene in T. reesei using the strong promoter Pcbh1 in T. reesei was proven triumphal in the simultaneous enhancement of the beta-exo synergism and exo-exo synergism in T. reesei cellulase.
30657063	4	107	theme	gene	795:798	arg1	co-expression					726:738	RESULTS Enhanced co-expression	709:738	RESULTS Enhanced co-expression of the β-glucosidase gene and the cellobiohydrolase II gene in T. reesei using the strong promoter Pcbh1	709:843	RESULTS Enhanced co-expression of the β-glucosidase gene and the cellobiohydrolase II gene in T. reesei using the strong promoter Pcbh1 was found successful in overcoming the two weaknesses.
30657063	10	108	theme	cellobiohydrolase	1657:1673	arg1	gene					1678:1681	the cellobiohydrolase II gene	1653:1681	the cellobiohydrolase II gene in T. reesei using the strong promoter Pcbh1 in T. reesei	1653:1739	CONCLUSIONS Enhanced co-expression of the β-glucosidase gene and the cellobiohydrolase II gene in T. reesei using the strong promoter Pcbh1 in T. reesei was proven triumphal in the simultaneous enhancement of the beta-exo synergism and exo-exo synergism in T. reesei cellulase.
30657063	0	109	theme	synergism	41:49	arg1	synergism					63:71	exo-exo synergism	55:71	exo-exo synergism in Trichoderma reesei cellulase	55:103	Simultaneous enhancement of the beta-exo synergism and exo-exo synergism in Trichoderma reesei cellulase to increase the cellulose degrading capability.
30657063	0	109	theme	synergism	41:49	arg1	enhancement					13:23	Simultaneous enhancement	0:23	Simultaneous enhancement of the beta-exo synergism	0:49	Simultaneous enhancement of the beta-exo synergism and exo-exo synergism in Trichoderma reesei cellulase to increase the cellulose degrading capability.
30657063	3	110	theme	cellobiohydrolase	669:685	arg1	gene					690:693	the cellobiohydrolase II gene	665:693	the cellobiohydrolase II gene of T. reesei	665:706	This work aimed at solving this problem by simultaneous enhancement of the beta-exo synergism and exo-exo synergism in T. reesei cellulase to increase the cellulose degrading capability, i.e. enhanced co-expression of the β-glucosidase gene the cellobiohydrolase II gene of T. reesei.
30657063	0	111	theme	exo-exo	55:61	arg1	synergism					63:71	exo-exo synergism	55:71	exo-exo synergism in Trichoderma reesei cellulase	55:103	Simultaneous enhancement of the beta-exo synergism and exo-exo synergism in Trichoderma reesei cellulase to increase the cellulose degrading capability.
30657063	8	112	theme	2.9637 g	1384:1391	arg1	SECS					1393:1396	2.9637 g SECS	1384:1396	2.9637 g SECS using Rut-C30 cellulase to 2.0291 g SECS using E7 cellulase and 2.0573 g SECS using F6 cellulase	1384:1493	The substrate loading for 1 g glucose release from SECS were decreased, from 2.9637 g SECS using Rut-C30 cellulase to 2.0291 g SECS using E7 cellulase and 2.0573 g SECS using F6 cellulase.
30657063	3	113	theme	exo-exo	522:528	arg1	synergism					530:538	exo-exo synergism	522:538	exo-exo synergism	522:538	This work aimed at solving this problem by simultaneous enhancement of the beta-exo synergism and exo-exo synergism in T. reesei cellulase to increase the cellulose degrading capability, i.e. enhanced co-expression of the β-glucosidase gene the cellobiohydrolase II gene of T. reesei.
30657063	2	114	theme	cellulase	309:317	arg1	producer					319:326	the most widely used cellulase producer	288:326	the most widely used cellulase producer	288:326	As the most widely used cellulase producer, Trichoderma reesei has two weaknesses, deficiencies in β-glucosidase and cellobiohydrolase II.
30657063	2	114	theme	cellulase	309:317	arg1	weaknesses					356:365	two weaknesses	352:365	two weaknesses	352:365	As the most widely used cellulase producer, Trichoderma reesei has two weaknesses, deficiencies in β-glucosidase and cellobiohydrolase II.
30657063	7	115	theme	E7	1259:1260	arg1	cellulase					1262:1270	E7 cellulase	1259:1270	E7 cellulase	1259:1270	Enzymatic hydrolysis yields were also improved, from 67.22 ± 1.61% by Rut-C30 cellulase to 87.98 ± 0.65% by E7 cellulase and 86.50 ± 1.01% by F6 cellulase.
30657063	4	116	theme	strong	823:828	arg1	Pcbh1					839:843	the strong promoter Pcbh1	819:843	the strong promoter Pcbh1	819:843	RESULTS Enhanced co-expression of the β-glucosidase gene and the cellobiohydrolase II gene in T. reesei using the strong promoter Pcbh1 was found successful in overcoming the two weaknesses.
30657063	3	117	theme	synergism	508:516	arg1	enhancement					480:490	simultaneous enhancement	467:490	simultaneous enhancement of the beta-exo synergism and exo-exo synergism in T. reesei cellulase to increase the cellulose degrading capability, i.e. enhanced co-expression of the β-glucosidase gene the cellobiohydrolase II gene of T. reesei	467:706	This work aimed at solving this problem by simultaneous enhancement of the beta-exo synergism and exo-exo synergism in T. reesei cellulase to increase the cellulose degrading capability, i.e. enhanced co-expression of the β-glucosidase gene the cellobiohydrolase II gene of T. reesei.
30657063	10	118	from	synergism	1832:1840	arg1	cellulase					1855:1863	T. reesei cellulase	1845:1863	T. reesei cellulase	1845:1863	CONCLUSIONS Enhanced co-expression of the β-glucosidase gene and the cellobiohydrolase II gene in T. reesei using the strong promoter Pcbh1 in T. reesei was proven triumphal in the simultaneous enhancement of the beta-exo synergism and exo-exo synergism in T. reesei cellulase.
30657063	10	118	from	synergism	1832:1840	arg1	triumphal					1752:1760	triumphal	1752:1760	triumphal	1752:1760	CONCLUSIONS Enhanced co-expression of the β-glucosidase gene and the cellobiohydrolase II gene in T. reesei using the strong promoter Pcbh1 in T. reesei was proven triumphal in the simultaneous enhancement of the beta-exo synergism and exo-exo synergism in T. reesei cellulase.
30657063	3	119	theme	reesei	546:551	arg1	cellulase					553:561	T. reesei cellulase	543:561	T. reesei cellulase	543:561	This work aimed at solving this problem by simultaneous enhancement of the beta-exo synergism and exo-exo synergism in T. reesei cellulase to increase the cellulose degrading capability, i.e. enhanced co-expression of the β-glucosidase gene the cellobiohydrolase II gene of T. reesei.
31135331	6	0	theme	DNA-DNA	786:792	arg1	%					823:823	23.3 and 31.0 %	809:823	23.3 and 31.0 %	809:823	Digital DNA-DNA hybridization (23.3 and 31.0 %) and average nucleotide identity (79.3 and 85.6 %) values between strain HST28T and its corresponding relatives mentioned above were below the threshold of 70 and 96 %, respectively, defined for assigning a prokaryotic strains to the same species.
31135331	6	0	theme	DNA-DNA	786:792	arg1	hybridization					794:806	Digital DNA-DNA hybridization	778:806	Digital DNA-DNA hybridization (23.3 and 31.0 %)	778:824	Digital DNA-DNA hybridization (23.3 and 31.0 %) and average nucleotide identity (79.3 and 85.6 %) values between strain HST28T and its corresponding relatives mentioned above were below the threshold of 70 and 96 %, respectively, defined for assigning a prokaryotic strains to the same species.
31135331	7	1	from	acid	1142:1145	arg1	layer					1168:1172	its peptidoglycan layer	1150:1172	its peptidoglycan layer	1150:1172	Strain HST28T was characterised by the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 : 0 and anteiso-C17 : 0 as major fatty acids (>15 %).
31135331	7	2	from	galactose	1175:1183	arg1	layer					1168:1172	its peptidoglycan layer	1150:1172	its peptidoglycan layer	1150:1172	Strain HST28T was characterised by the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 : 0 and anteiso-C17 : 0 as major fatty acids (>15 %).
31135331	2	3	dep	Salar	190:194	arg1	de					196:197	Salar de	190:197	the Salar de Huasco	186:204	Streptomyces strain HST28T isolated from the Salar de Huasco, an athalassohaline and poly-extreme high altitude saline wetland located in northern Chile, was the subject of a polyphasic taxonomic study.
31135331	6	4	theme	identity	849:856	arg1	values					876:881	Digital DNA-DNA hybridization (23.3 and 31.0 %) and average nucleotide identity (79.3 and 85.6 %) values	778:881	Digital DNA-DNA hybridization (23.3 and 31.0 %) and average nucleotide identity (79.3 and 85.6 %) values between strain HST28T and its corresponding relatives mentioned above	778:951	Digital DNA-DNA hybridization (23.3 and 31.0 %) and average nucleotide identity (79.3 and 85.6 %) values between strain HST28T and its corresponding relatives mentioned above were below the threshold of 70 and 96 %, respectively, defined for assigning a prokaryotic strains to the same species.
31135331	7	5	from	glucose	1186:1192	arg1	layer					1168:1172	its peptidoglycan layer	1150:1172	its peptidoglycan layer	1150:1172	Strain HST28T was characterised by the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 : 0 and anteiso-C17 : 0 as major fatty acids (>15 %).
31135331	7	6	theme	polar	1373:1377	arg1	lipids					1379:1384	polar lipids	1373:1384	polar lipids	1373:1384	Strain HST28T was characterised by the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 : 0 and anteiso-C17 : 0 as major fatty acids (>15 %).
31135331	2	7	theme	Streptomyces	145:156	arg1	wetland					264:270	poly-extreme high altitude saline wetland	230:270	poly-extreme high altitude saline wetland	230:270	Streptomyces strain HST28T isolated from the Salar de Huasco, an athalassohaline and poly-extreme high altitude saline wetland located in northern Chile, was the subject of a polyphasic taxonomic study.
31135331	2	7	theme	Streptomyces	145:156	arg1	athalassohaline					210:224	athalassohaline	210:224	athalassohaline	210:224	Streptomyces strain HST28T isolated from the Salar de Huasco, an athalassohaline and poly-extreme high altitude saline wetland located in northern Chile, was the subject of a polyphasic taxonomic study.
31135331	2	7	theme	Streptomyces	145:156	arg1	subject					307:313	the subject	303:313	the subject of a polyphasic taxonomic study	303:345	Streptomyces strain HST28T isolated from the Salar de Huasco, an athalassohaline and poly-extreme high altitude saline wetland located in northern Chile, was the subject of a polyphasic taxonomic study.
31135331	2	7	theme	Streptomyces	145:156	arg1	strain					158:163	Streptomyces strain HST28T	145:170	Streptomyces strain HST28T isolated from the Salar de Huasco	145:204	Streptomyces strain HST28T isolated from the Salar de Huasco, an athalassohaline and poly-extreme high altitude saline wetland located in northern Chile, was the subject of a polyphasic taxonomic study.
31135331	8	8	theme	phenotypic	1552:1561	arg1	results					1575:1581	the phenotypic and genetic results	1548:1581	the phenotypic and genetic results	1548:1581	Based on the phenotypic and genetic results, strain HST28T (DSM 107268T=CECT 9648T) merits recognition as a new species named Streptomyces huasconensis sp.
31135331	6	9	theme	average	830:836	arg1	identity					849:856	average nucleotide identity	830:856	average nucleotide identity (79.3 and 85.6 %)	830:874	Digital DNA-DNA hybridization (23.3 and 31.0 %) and average nucleotide identity (79.3 and 85.6 %) values between strain HST28T and its corresponding relatives mentioned above were below the threshold of 70 and 96 %, respectively, defined for assigning a prokaryotic strains to the same species.
31135331	6	9	theme	average	830:836	arg1	%					873:873	79.3 and 85.6 %	859:873	79.3 and 85.6 %	859:873	Digital DNA-DNA hybridization (23.3 and 31.0 %) and average nucleotide identity (79.3 and 85.6 %) values between strain HST28T and its corresponding relatives mentioned above were below the threshold of 70 and 96 %, respectively, defined for assigning a prokaryotic strains to the same species.
31135331	7	10	from	ribose	1195:1200	arg1	layer					1168:1172	its peptidoglycan layer	1150:1172	its peptidoglycan layer	1150:1172	Strain HST28T was characterised by the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 : 0 and anteiso-C17 : 0 as major fatty acids (>15 %).
31135331	7	11	theme	lipid	1364:1368	arg1	presence					1112:1119	the presence	1108:1119	the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 	1108:1484	Strain HST28T was characterised by the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 : 0 and anteiso-C17 : 0 as major fatty acids (>15 %).
31135331	1	12	attach	isolated	74:81	arg2	actinobacterium					58:72	an haloalkalitolerant actinobacterium	36:72	an haloalkalitolerant actinobacterium isolated from a high altitude saline	36:109	nov., an haloalkalitolerant actinobacterium isolated from a high altitude saline wetland at the Chilean Altiplano.
31135331	1	12	attach	isolated	74:81	arg1	saline					104:109	a high altitude saline	88:109	a high altitude saline	88:109	nov., an haloalkalitolerant actinobacterium isolated from a high altitude saline wetland at the Chilean Altiplano.
31135331	1	12	attach	isolated	74:81	arg2	nov.					30:33	nov.	30:33	nov.	30:33	nov., an haloalkalitolerant actinobacterium isolated from a high altitude saline wetland at the Chilean Altiplano.
31135331	5	13	theme	gene	593:596	arg1	analyses					607:614	Single (16S rRNA) and multi-locus gene sequence analyses	559:614	analyses	607:614	Single (16S rRNA) and multi-locus gene sequence analyses showed that strain HST28T had a distinct phylogenetic position from its closest relatives, the type strains of Steptomyces aureus and Streptomyces kanamyceticus.
31135331	8	14	theme	genetic	1567:1573	arg1	results					1575:1581	the phenotypic and genetic results	1548:1581	the phenotypic and genetic results	1548:1581	Based on the phenotypic and genetic results, strain HST28T (DSM 107268T=CECT 9648T) merits recognition as a new species named Streptomyces huasconensis sp.
31135331	7	15	from	traces	1206:1211	arg1	layer					1168:1172	its peptidoglycan layer	1150:1172	its peptidoglycan layer	1150:1172	Strain HST28T was characterised by the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 : 0 and anteiso-C17 : 0 as major fatty acids (>15 %).
31135331	5	16	theme	phylogenetic	657:668	arg1	position					670:677	a distinct phylogenetic position	646:677	a distinct phylogenetic position	646:677	Single (16S rRNA) and multi-locus gene sequence analyses showed that strain HST28T had a distinct phylogenetic position from its closest relatives, the type strains of Steptomyces aureus and Streptomyces kanamyceticus.
31135331	7	17	theme	glycophospholipid	1326:1342	arg1	presence					1112:1119	the presence	1108:1119	the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 	1108:1484	Strain HST28T was characterised by the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 : 0 and anteiso-C17 : 0 as major fatty acids (>15 %).
31135331	2	18	theme	poly-extreme	230:241	arg1	strain					158:163	Streptomyces strain HST28T	145:170	Streptomyces strain HST28T isolated from the Salar de Huasco	145:204	Streptomyces strain HST28T isolated from the Salar de Huasco, an athalassohaline and poly-extreme high altitude saline wetland located in northern Chile, was the subject of a polyphasic taxonomic study.
31135331	2	18	theme	poly-extreme	230:241	arg1	wetland					264:270	poly-extreme high altitude saline wetland	230:270	poly-extreme high altitude saline wetland	230:270	Streptomyces strain HST28T isolated from the Salar de Huasco, an athalassohaline and poly-extreme high altitude saline wetland located in northern Chile, was the subject of a polyphasic taxonomic study.
31135331	7	19	theme	major	1512:1516	arg1	acids					1524:1528	major fatty acids	1512:1528	major fatty acids (>15 %)	1512:1536	Strain HST28T was characterised by the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 : 0 and anteiso-C17 : 0 as major fatty acids (>15 %).
31135331	7	19	theme	major	1512:1516	arg1	%					1535:1535	>15 %	1531:1535	>15 %	1531:1535	Strain HST28T was characterised by the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 : 0 and anteiso-C17 : 0 as major fatty acids (>15 %).
31135331	4	20	theme	Optimal	470:476	arg1	growth					478:483	Optimal growth	470:483	Optimal growth of strain HST28T	470:500	Optimal growth of strain HST28T was obtained at 28 °C, pH 8-9 and up to 10 % (w/v) NaCl.
31135331	7	21	theme	aminolipid	1314:1323	arg1	presence					1112:1119	the presence	1108:1119	the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 	1108:1484	Strain HST28T was characterised by the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 : 0 and anteiso-C17 : 0 as major fatty acids (>15 %).
31135331	8	22	theme	DSM	1599:1601	arg1	9648T					1616:1620	DSM 107268T=CECT 9648T	1599:1620	DSM 107268T=CECT 9648T	1599:1620	Based on the phenotypic and genetic results, strain HST28T (DSM 107268T=CECT 9648T) merits recognition as a new species named Streptomyces huasconensis sp.
31135331	8	22	theme	DSM	1599:1601	arg1	HST28T					1591:1596	strain HST28T	1584:1596	strain HST28T (DSM 107268T=CECT 9648T)	1584:1621	Based on the phenotypic and genetic results, strain HST28T (DSM 107268T=CECT 9648T) merits recognition as a new species named Streptomyces huasconensis sp.
31135331	7	23	theme	phosphatidylinositol	1292:1311	arg1	presence					1112:1119	the presence	1108:1119	the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 	1108:1484	Strain HST28T was characterised by the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 : 0 and anteiso-C17 : 0 as major fatty acids (>15 %).
31135331	7	24	from	lipid	1364:1368	arg1	layer					1168:1172	its peptidoglycan layer	1150:1172	its peptidoglycan layer	1150:1172	Strain HST28T was characterised by the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 : 0 and anteiso-C17 : 0 as major fatty acids (>15 %).
31135331	7	25	from	layer	1168:1172	arg1	presence					1112:1119	the presence	1108:1119	the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 	1108:1484	Strain HST28T was characterised by the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 : 0 and anteiso-C17 : 0 as major fatty acids (>15 %).
31135331	7	26	theme	phosphatidylmethylethanolamine	1260:1289	arg1	presence					1112:1119	the presence	1108:1119	the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 	1108:1484	Strain HST28T was characterised by the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 : 0 and anteiso-C17 : 0 as major fatty acids (>15 %).
31135331	5	27	theme	type	711:714	arg1	relatives					696:704	its closest relatives	684:704	its closest relatives	684:704	Single (16S rRNA) and multi-locus gene sequence analyses showed that strain HST28T had a distinct phylogenetic position from its closest relatives, the type strains of Steptomyces aureus and Streptomyces kanamyceticus.
31135331	5	27	theme	type	711:714	arg1	strains					716:722	the type strains	707:722	the type strains of Steptomyces aureus and Streptomyces kanamyceticus	707:775	Single (16S rRNA) and multi-locus gene sequence analyses showed that strain HST28T had a distinct phylogenetic position from its closest relatives, the type strains of Steptomyces aureus and Streptomyces kanamyceticus.
31135331	2	28	theme	altitude	248:255	arg1	strain					158:163	Streptomyces strain HST28T	145:170	Streptomyces strain HST28T isolated from the Salar de Huasco	145:204	Streptomyces strain HST28T isolated from the Salar de Huasco, an athalassohaline and poly-extreme high altitude saline wetland located in northern Chile, was the subject of a polyphasic taxonomic study.
31135331	2	28	theme	altitude	248:255	arg1	wetland					264:270	poly-extreme high altitude saline wetland	230:270	poly-extreme high altitude saline wetland	230:270	Streptomyces strain HST28T isolated from the Salar de Huasco, an athalassohaline and poly-extreme high altitude saline wetland located in northern Chile, was the subject of a polyphasic taxonomic study.
31135331	5	29	theme	closest	688:694	arg1	relatives					696:704	its closest relatives	684:704	its closest relatives	684:704	Single (16S rRNA) and multi-locus gene sequence analyses showed that strain HST28T had a distinct phylogenetic position from its closest relatives, the type strains of Steptomyces aureus and Streptomyces kanamyceticus.
31135331	5	29	theme	closest	688:694	arg1	strains					716:722	the type strains	707:722	the type strains of Steptomyces aureus and Streptomyces kanamyceticus	707:775	Single (16S rRNA) and multi-locus gene sequence analyses showed that strain HST28T had a distinct phylogenetic position from its closest relatives, the type strains of Steptomyces aureus and Streptomyces kanamyceticus.
31135331	5	30	theme	16S	567:569	arg1	Single					559:564	Single	559:564	Single	559:564	Single (16S rRNA) and multi-locus gene sequence analyses showed that strain HST28T had a distinct phylogenetic position from its closest relatives, the type strains of Steptomyces aureus and Streptomyces kanamyceticus.
31135331	5	30	theme	16S	567:569	arg1	rRNA					571:574	16S rRNA	567:574	16S rRNA	567:574	Single (16S rRNA) and multi-locus gene sequence analyses showed that strain HST28T had a distinct phylogenetic position from its closest relatives, the type strains of Steptomyces aureus and Streptomyces kanamyceticus.
31135331	2	31	from	located	272:278	arg1	Chile					292:296	northern Chile	283:296	northern Chile	283:296	Streptomyces strain HST28T isolated from the Salar de Huasco, an athalassohaline and poly-extreme high altitude saline wetland located in northern Chile, was the subject of a polyphasic taxonomic study.
31135331	7	32	from	phosphatidylmethylethanolamine	1260:1289	arg1	layer					1168:1172	its peptidoglycan layer	1150:1172	its peptidoglycan layer	1150:1172	Strain HST28T was characterised by the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 : 0 and anteiso-C17 : 0 as major fatty acids (>15 %).
31135331	4	33	dep	10 	542:544	arg1	to					539:540	to	539:540	to	539:540	Optimal growth of strain HST28T was obtained at 28 °C, pH 8-9 and up to 10 % (w/v) NaCl.
31135331	7	34	from	phosphatidylinositol	1292:1311	arg1	layer					1168:1172	its peptidoglycan layer	1150:1172	its peptidoglycan layer	1150:1172	Strain HST28T was characterised by the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 : 0 and anteiso-C17 : 0 as major fatty acids (>15 %).
31135331	3	35	theme	chemotaxonomic	387:400	arg1	features					402:409	morphological and chemotaxonomic features	369:409	morphological and chemotaxonomic features	369:409	Strain HST28T showed morphological and chemotaxonomic features in line with its classification in the genus Streptomyces.
31135331	4	36	theme	HST28T	495:500	arg1	growth					478:483	Optimal growth	470:483	Optimal growth of strain HST28T	470:500	Optimal growth of strain HST28T was obtained at 28 °C, pH 8-9 and up to 10 % (w/v) NaCl.
31135331	0	37	theme	Streptomyces	0:11	arg1	sp					26:27	Streptomyces huasconensis sp	0:27	Streptomyces huasconensis sp.	0:28	Streptomyces huasconensis sp.
31135331	8	38	theme	huasconensis	1678:1689	arg1	sp					1691:1692	Streptomyces huasconensis sp	1665:1692	Streptomyces huasconensis sp	1665:1692	Based on the phenotypic and genetic results, strain HST28T (DSM 107268T=CECT 9648T) merits recognition as a new species named Streptomyces huasconensis sp.
31135331	1	39	theme	altitude	95:102	arg1	saline					104:109	a high altitude saline	88:109	a high altitude saline	88:109	nov., an haloalkalitolerant actinobacterium isolated from a high altitude saline wetland at the Chilean Altiplano.
31135331	7	40	from	aminolipid	1314:1323	arg1	layer					1168:1172	its peptidoglycan layer	1150:1172	its peptidoglycan layer	1150:1172	Strain HST28T was characterised by the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 : 0 and anteiso-C17 : 0 as major fatty acids (>15 %).
31135331	7	41	theme	whole-cell	1241:1250	arg1	sugars					1252:1257	whole-cell sugars	1241:1257	whole-cell sugars	1241:1257	Strain HST28T was characterised by the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 : 0 and anteiso-C17 : 0 as major fatty acids (>15 %).
31135331	3	42	theme	morphological	369:381	arg1	features					402:409	morphological and chemotaxonomic features	369:409	morphological and chemotaxonomic features	369:409	Strain HST28T showed morphological and chemotaxonomic features in line with its classification in the genus Streptomyces.
31135331	7	43	from	glycophospholipid	1326:1342	arg1	layer					1168:1172	its peptidoglycan layer	1150:1172	its peptidoglycan layer	1150:1172	Strain HST28T was characterised by the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 : 0 and anteiso-C17 : 0 as major fatty acids (>15 %).
31135331	5	44	theme	aureus	739:744	arg1	relatives					696:704	its closest relatives	684:704	its closest relatives	684:704	Single (16S rRNA) and multi-locus gene sequence analyses showed that strain HST28T had a distinct phylogenetic position from its closest relatives, the type strains of Steptomyces aureus and Streptomyces kanamyceticus.
31135331	5	44	theme	aureus	739:744	arg1	strains					716:722	the type strains	707:722	the type strains of Steptomyces aureus and Streptomyces kanamyceticus	707:775	Single (16S rRNA) and multi-locus gene sequence analyses showed that strain HST28T had a distinct phylogenetic position from its closest relatives, the type strains of Steptomyces aureus and Streptomyces kanamyceticus.
31135331	7	45	theme	arabinose	1216:1224	arg1	lipid					1364:1368	an unidentified lipid	1348:1368	an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %)	1348:1460	Strain HST28T was characterised by the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 : 0 and anteiso-C17 : 0 as major fatty acids (>15 %).
31135331	7	45	theme	arabinose	1216:1224	arg1	acid					1142:1145	ll-diaminopimelic acid	1124:1145	ll-diaminopimelic acid in its peptidoglycan layer	1124:1172	Strain HST28T was characterised by the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 : 0 and anteiso-C17 : 0 as major fatty acids (>15 %).
31135331	7	45	theme	arabinose	1216:1224	arg1	galactose					1175:1183	galactose	1175:1183	galactose	1175:1183	Strain HST28T was characterised by the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 : 0 and anteiso-C17 : 0 as major fatty acids (>15 %).
31135331	7	45	theme	arabinose	1216:1224	arg1	aminolipid					1314:1323	aminolipid	1314:1323	aminolipid	1314:1323	Strain HST28T was characterised by the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 : 0 and anteiso-C17 : 0 as major fatty acids (>15 %).
31135331	7	45	theme	arabinose	1216:1224	arg1	glucose					1186:1192	glucose	1186:1192	glucose	1186:1192	Strain HST28T was characterised by the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 : 0 and anteiso-C17 : 0 as major fatty acids (>15 %).
31135331	7	45	theme	arabinose	1216:1224	arg1	glycophospholipid					1326:1342	glycophospholipid	1326:1342	glycophospholipid	1326:1342	Strain HST28T was characterised by the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 : 0 and anteiso-C17 : 0 as major fatty acids (>15 %).
31135331	7	45	theme	arabinose	1216:1224	arg1	phosphatidylmethylethanolamine					1260:1289	phosphatidylmethylethanolamine	1260:1289	phosphatidylmethylethanolamine	1260:1289	Strain HST28T was characterised by the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 : 0 and anteiso-C17 : 0 as major fatty acids (>15 %).
31135331	7	45	theme	arabinose	1216:1224	arg1	mannose					1230:1236	mannose	1230:1236	mannose as whole-cell sugars	1230:1257	Strain HST28T was characterised by the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 : 0 and anteiso-C17 : 0 as major fatty acids (>15 %).
31135331	7	45	theme	arabinose	1216:1224	arg1	phosphatidylinositol					1292:1311	phosphatidylinositol	1292:1311	phosphatidylinositol	1292:1311	Strain HST28T was characterised by the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 : 0 and anteiso-C17 : 0 as major fatty acids (>15 %).
31135331	7	45	theme	arabinose	1216:1224	arg1	ribose					1195:1200	ribose	1195:1200	ribose	1195:1200	Strain HST28T was characterised by the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 : 0 and anteiso-C17 : 0 as major fatty acids (>15 %).
31135331	7	45	theme	arabinose	1216:1224	arg1	anteiso-C15 					1473:1484	anteiso-C15 	1473:1484	anteiso-C15 	1473:1484	Strain HST28T was characterised by the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 : 0 and anteiso-C17 : 0 as major fatty acids (>15 %).
31135331	7	45	theme	arabinose	1216:1224	arg1	traces					1206:1211	traces	1206:1211	traces	1206:1211	Strain HST28T was characterised by the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 : 0 and anteiso-C17 : 0 as major fatty acids (>15 %).
31135331	5	46	contain	had	642:644	arg2	position					670:677	a distinct phylogenetic position	646:677	a distinct phylogenetic position	646:677	Single (16S rRNA) and multi-locus gene sequence analyses showed that strain HST28T had a distinct phylogenetic position from its closest relatives, the type strains of Steptomyces aureus and Streptomyces kanamyceticus.
31135331	5	46	contain	had	642:644	arg1	HST28T					635:640	strain HST28T	628:640	strain HST28T	628:640	Single (16S rRNA) and multi-locus gene sequence analyses showed that strain HST28T had a distinct phylogenetic position from its closest relatives, the type strains of Steptomyces aureus and Streptomyces kanamyceticus.
31135331	2	47	theme	study	341:345	arg1	strain					158:163	Streptomyces strain HST28T	145:170	Streptomyces strain HST28T isolated from the Salar de Huasco	145:204	Streptomyces strain HST28T isolated from the Salar de Huasco, an athalassohaline and poly-extreme high altitude saline wetland located in northern Chile, was the subject of a polyphasic taxonomic study.
31135331	2	47	theme	study	341:345	arg1	subject					307:313	the subject	303:313	the subject of a polyphasic taxonomic study	303:345	Streptomyces strain HST28T isolated from the Salar de Huasco, an athalassohaline and poly-extreme high altitude saline wetland located in northern Chile, was the subject of a polyphasic taxonomic study.
31135331	7	48	dep	menaquinones	1409:1420	arg1	MK-9					1445:1448	MK-9	1445:1448	MK-9	1445:1448	Strain HST28T was characterised by the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 : 0 and anteiso-C17 : 0 as major fatty acids (>15 %).
31135331	7	48	dep	menaquinones	1409:1420	arg1	H4					1450:1451	H4	1450:1451	H4	1450:1451	Strain HST28T was characterised by the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 : 0 and anteiso-C17 : 0 as major fatty acids (>15 %).
31135331	7	48	dep	menaquinones	1409:1420	arg1	menaquinones					1409:1420	the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4)	1391:1452	the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %)	1391:1460	Strain HST28T was characterised by the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 : 0 and anteiso-C17 : 0 as major fatty acids (>15 %).
31135331	7	48	dep	menaquinones	1409:1420	arg1	MK-9					1432:1435	MK-9	1432:1435	MK-9(H8)	1432:1439	Strain HST28T was characterised by the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 : 0 and anteiso-C17 : 0 as major fatty acids (>15 %).
31135331	7	48	dep	menaquinones	1409:1420	arg1	MK-9					1422:1425	MK-9	1422:1425	MK-9(H6)	1422:1429	Strain HST28T was characterised by the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 : 0 and anteiso-C17 : 0 as major fatty acids (>15 %).
31135331	7	48	dep	menaquinones	1409:1420	arg1	H8					1437:1438	H8	1437:1438	H8	1437:1438	Strain HST28T was characterised by the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 : 0 and anteiso-C17 : 0 as major fatty acids (>15 %).
31135331	7	48	dep	menaquinones	1409:1420	arg1	H6					1427:1428	H6	1427:1428	H6	1427:1428	Strain HST28T was characterised by the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 : 0 and anteiso-C17 : 0 as major fatty acids (>15 %).
31135331	2	49	theme	polyphasic	320:329	arg1	study					341:345	a polyphasic taxonomic study	318:345	a polyphasic taxonomic study	318:345	Streptomyces strain HST28T isolated from the Salar de Huasco, an athalassohaline and poly-extreme high altitude saline wetland located in northern Chile, was the subject of a polyphasic taxonomic study.
31135331	1	50	theme	haloalkalitolerant	39:56	arg1	actinobacterium					58:72	an haloalkalitolerant actinobacterium	36:72	an haloalkalitolerant actinobacterium isolated from a high altitude saline	36:109	nov., an haloalkalitolerant actinobacterium isolated from a high altitude saline wetland at the Chilean Altiplano.
31135331	1	50	theme	haloalkalitolerant	39:56	arg1	nov.					30:33	nov.	30:33	nov.	30:33	nov., an haloalkalitolerant actinobacterium isolated from a high altitude saline wetland at the Chilean Altiplano.
31135331	5	51	theme	sequence	598:605	arg1	analyses					607:614	Single (16S rRNA) and multi-locus gene sequence analyses	559:614	analyses	607:614	Single (16S rRNA) and multi-locus gene sequence analyses showed that strain HST28T had a distinct phylogenetic position from its closest relatives, the type strains of Steptomyces aureus and Streptomyces kanamyceticus.
31135331	7	52	theme	predominating	1395:1407	arg1	menaquinones					1409:1420	the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4)	1391:1452	the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %)	1391:1460	Strain HST28T was characterised by the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 : 0 and anteiso-C17 : 0 as major fatty acids (>15 %).
31135331	7	52	theme	predominating	1395:1407	arg1	MK-9					1445:1448	MK-9	1445:1448	MK-9	1445:1448	Strain HST28T was characterised by the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 : 0 and anteiso-C17 : 0 as major fatty acids (>15 %).
31135331	7	52	theme	predominating	1395:1407	arg1	%					1459:1459	>20 %	1455:1459	>20 %	1455:1459	Strain HST28T was characterised by the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 : 0 and anteiso-C17 : 0 as major fatty acids (>15 %).
31135331	7	52	theme	predominating	1395:1407	arg1	MK-9					1422:1425	MK-9	1422:1425	MK-9(H6)	1422:1429	Strain HST28T was characterised by the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 : 0 and anteiso-C17 : 0 as major fatty acids (>15 %).
31135331	7	52	theme	predominating	1395:1407	arg1	MK-9					1432:1435	MK-9	1432:1435	MK-9(H8)	1432:1439	Strain HST28T was characterised by the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 : 0 and anteiso-C17 : 0 as major fatty acids (>15 %).
31135331	7	53	theme	galactose	1175:1183	arg1	presence					1112:1119	the presence	1108:1119	the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 	1108:1484	Strain HST28T was characterised by the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 : 0 and anteiso-C17 : 0 as major fatty acids (>15 %).
31135331	6	54	theme	Digital	778:784	arg1	%					823:823	23.3 and 31.0 %	809:823	23.3 and 31.0 %	809:823	Digital DNA-DNA hybridization (23.3 and 31.0 %) and average nucleotide identity (79.3 and 85.6 %) values between strain HST28T and its corresponding relatives mentioned above were below the threshold of 70 and 96 %, respectively, defined for assigning a prokaryotic strains to the same species.
31135331	6	54	theme	Digital	778:784	arg1	hybridization					794:806	Digital DNA-DNA hybridization	778:806	Digital DNA-DNA hybridization (23.3 and 31.0 %)	778:824	Digital DNA-DNA hybridization (23.3 and 31.0 %) and average nucleotide identity (79.3 and 85.6 %) values between strain HST28T and its corresponding relatives mentioned above were below the threshold of 70 and 96 %, respectively, defined for assigning a prokaryotic strains to the same species.
31135331	3	55	with	line	414:417	arg1	classification					428:441	its classification	424:441	its classification in the genus Streptomyces	424:467	Strain HST28T showed morphological and chemotaxonomic features in line with its classification in the genus Streptomyces.
31135331	2	56	theme	northern	283:290	arg1	Chile					292:296	northern Chile	283:296	northern Chile	283:296	Streptomyces strain HST28T isolated from the Salar de Huasco, an athalassohaline and poly-extreme high altitude saline wetland located in northern Chile, was the subject of a polyphasic taxonomic study.
31135331	6	57	theme	nucleotide	838:847	arg1	identity					849:856	average nucleotide identity	830:856	average nucleotide identity (79.3 and 85.6 %)	830:874	Digital DNA-DNA hybridization (23.3 and 31.0 %) and average nucleotide identity (79.3 and 85.6 %) values between strain HST28T and its corresponding relatives mentioned above were below the threshold of 70 and 96 %, respectively, defined for assigning a prokaryotic strains to the same species.
31135331	6	57	theme	nucleotide	838:847	arg1	%					873:873	79.3 and 85.6 %	859:873	79.3 and 85.6 %	859:873	Digital DNA-DNA hybridization (23.3 and 31.0 %) and average nucleotide identity (79.3 and 85.6 %) values between strain HST28T and its corresponding relatives mentioned above were below the threshold of 70 and 96 %, respectively, defined for assigning a prokaryotic strains to the same species.
31135331	5	58	theme	multi-locus	581:591	arg1	analyses					607:614	Single (16S rRNA) and multi-locus gene sequence analyses	559:614	analyses	607:614	Single (16S rRNA) and multi-locus gene sequence analyses showed that strain HST28T had a distinct phylogenetic position from its closest relatives, the type strains of Steptomyces aureus and Streptomyces kanamyceticus.
31135331	6	59	theme	corresponding	913:925	arg1	relatives					927:935	its corresponding relatives	909:935	its corresponding relatives	909:935	Digital DNA-DNA hybridization (23.3 and 31.0 %) and average nucleotide identity (79.3 and 85.6 %) values between strain HST28T and its corresponding relatives mentioned above were below the threshold of 70 and 96 %, respectively, defined for assigning a prokaryotic strains to the same species.
31135331	5	60	theme	distinct	648:655	arg1	position					670:677	a distinct phylogenetic position	646:677	a distinct phylogenetic position	646:677	Single (16S rRNA) and multi-locus gene sequence analyses showed that strain HST28T had a distinct phylogenetic position from its closest relatives, the type strains of Steptomyces aureus and Streptomyces kanamyceticus.
31135331	7	61	from	presence	1112:1119	arg1	layer					1168:1172	its peptidoglycan layer	1150:1172	its peptidoglycan layer	1150:1172	Strain HST28T was characterised by the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 : 0 and anteiso-C17 : 0 as major fatty acids (>15 %).
31135331	7	62	theme	unidentified	1351:1362	arg1	lipid					1364:1368	an unidentified lipid	1348:1368	an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %)	1348:1460	Strain HST28T was characterised by the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 : 0 and anteiso-C17 : 0 as major fatty acids (>15 %).
31135331	2	63	dep	athalassohaline	210:224	arg1	an					207:208	an	207:208	an	207:208	Streptomyces strain HST28T isolated from the Salar de Huasco, an athalassohaline and poly-extreme high altitude saline wetland located in northern Chile, was the subject of a polyphasic taxonomic study.
31135331	4	64	dep	NaCl	553:556	arg1	%					545:545	%	545:545	%	545:545	Optimal growth of strain HST28T was obtained at 28 °C, pH 8-9 and up to 10 % (w/v) NaCl.
31135331	2	65	theme	high	243:246	arg1	strain					158:163	Streptomyces strain HST28T	145:170	Streptomyces strain HST28T isolated from the Salar de Huasco	145:204	Streptomyces strain HST28T isolated from the Salar de Huasco, an athalassohaline and poly-extreme high altitude saline wetland located in northern Chile, was the subject of a polyphasic taxonomic study.
31135331	2	65	theme	high	243:246	arg1	wetland					264:270	poly-extreme high altitude saline wetland	230:270	poly-extreme high altitude saline wetland	230:270	Streptomyces strain HST28T isolated from the Salar de Huasco, an athalassohaline and poly-extreme high altitude saline wetland located in northern Chile, was the subject of a polyphasic taxonomic study.
31135331	6	66	theme	strain	891:896	arg1	HST28T					898:903	strain HST28T	891:903	strain HST28T	891:903	Digital DNA-DNA hybridization (23.3 and 31.0 %) and average nucleotide identity (79.3 and 85.6 %) values between strain HST28T and its corresponding relatives mentioned above were below the threshold of 70 and 96 %, respectively, defined for assigning a prokaryotic strains to the same species.
31135331	5	67	theme	strain	628:633	arg1	HST28T					635:640	strain HST28T	628:640	strain HST28T	628:640	Single (16S rRNA) and multi-locus gene sequence analyses showed that strain HST28T had a distinct phylogenetic position from its closest relatives, the type strains of Steptomyces aureus and Streptomyces kanamyceticus.
31135331	8	68	theme	strain	1584:1589	arg1	9648T					1616:1620	DSM 107268T=CECT 9648T	1599:1620	DSM 107268T=CECT 9648T	1599:1620	Based on the phenotypic and genetic results, strain HST28T (DSM 107268T=CECT 9648T) merits recognition as a new species named Streptomyces huasconensis sp.
31135331	8	68	theme	strain	1584:1589	arg1	HST28T					1591:1596	strain HST28T	1584:1596	strain HST28T (DSM 107268T=CECT 9648T)	1584:1621	Based on the phenotypic and genetic results, strain HST28T (DSM 107268T=CECT 9648T) merits recognition as a new species named Streptomyces huasconensis sp.
31135331	7	69	theme	anteiso-C15 	1473:1484	arg1	presence					1112:1119	the presence	1108:1119	the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 	1108:1484	Strain HST28T was characterised by the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 : 0 and anteiso-C17 : 0 as major fatty acids (>15 %).
31135331	2	70	theme	located	272:278	arg1	athalassohaline					210:224	athalassohaline	210:224	athalassohaline	210:224	Streptomyces strain HST28T isolated from the Salar de Huasco, an athalassohaline and poly-extreme high altitude saline wetland located in northern Chile, was the subject of a polyphasic taxonomic study.
31135331	2	70	theme	located	272:278	arg1	strain					158:163	Streptomyces strain HST28T	145:170	Streptomyces strain HST28T isolated from the Salar de Huasco	145:204	Streptomyces strain HST28T isolated from the Salar de Huasco, an athalassohaline and poly-extreme high altitude saline wetland located in northern Chile, was the subject of a polyphasic taxonomic study.
31135331	7	71	theme	peptidoglycan	1154:1166	arg1	layer					1168:1172	its peptidoglycan layer	1150:1172	its peptidoglycan layer	1150:1172	Strain HST28T was characterised by the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 : 0 and anteiso-C17 : 0 as major fatty acids (>15 %).
31135331	2	72	theme	saline	257:262	arg1	strain					158:163	Streptomyces strain HST28T	145:170	Streptomyces strain HST28T isolated from the Salar de Huasco	145:204	Streptomyces strain HST28T isolated from the Salar de Huasco, an athalassohaline and poly-extreme high altitude saline wetland located in northern Chile, was the subject of a polyphasic taxonomic study.
31135331	2	72	theme	saline	257:262	arg1	wetland					264:270	poly-extreme high altitude saline wetland	230:270	poly-extreme high altitude saline wetland	230:270	Streptomyces strain HST28T isolated from the Salar de Huasco, an athalassohaline and poly-extreme high altitude saline wetland located in northern Chile, was the subject of a polyphasic taxonomic study.
31135331	8	73	theme	107268T=CECT	1603:1614	arg1	9648T					1616:1620	DSM 107268T=CECT 9648T	1599:1620	DSM 107268T=CECT 9648T	1599:1620	Based on the phenotypic and genetic results, strain HST28T (DSM 107268T=CECT 9648T) merits recognition as a new species named Streptomyces huasconensis sp.
31135331	8	73	theme	107268T=CECT	1603:1614	arg1	HST28T					1591:1596	strain HST28T	1584:1596	strain HST28T (DSM 107268T=CECT 9648T)	1584:1621	Based on the phenotypic and genetic results, strain HST28T (DSM 107268T=CECT 9648T) merits recognition as a new species named Streptomyces huasconensis sp.
31135331	2	74	from	Chile	292:296	arg1	located					272:278	located	272:278	located	272:278	Streptomyces strain HST28T isolated from the Salar de Huasco, an athalassohaline and poly-extreme high altitude saline wetland located in northern Chile, was the subject of a polyphasic taxonomic study.
31135331	7	75	theme	acid	1142:1145	arg1	presence					1112:1119	the presence	1108:1119	the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 	1108:1484	Strain HST28T was characterised by the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 : 0 and anteiso-C17 : 0 as major fatty acids (>15 %).
31135331	7	76	from	anteiso-C15 	1473:1484	arg1	layer					1168:1172	its peptidoglycan layer	1150:1172	its peptidoglycan layer	1150:1172	Strain HST28T was characterised by the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 : 0 and anteiso-C17 : 0 as major fatty acids (>15 %).
31135331	7	77	dep	characterised	1091:1103	arg1	 0					1486:1487	 0	1486:1487	 0	1486:1487	Strain HST28T was characterised by the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 : 0 and anteiso-C17 : 0 as major fatty acids (>15 %).
31135331	7	77	dep	characterised	1091:1103	arg1	 0					1506:1507	 0	1506:1507	 0 as major fatty acids (>15 %)	1506:1536	Strain HST28T was characterised by the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 : 0 and anteiso-C17 : 0 as major fatty acids (>15 %).
31135331	7	77	dep	characterised	1091:1103	arg1	anteiso-C17 					1493:1504	anteiso-C17 	1493:1504	anteiso-C17 	1493:1504	Strain HST28T was characterised by the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 : 0 and anteiso-C17 : 0 as major fatty acids (>15 %).
31135331	7	78	theme	ll-diaminopimelic	1124:1140	arg1	acid					1142:1145	ll-diaminopimelic acid	1124:1145	ll-diaminopimelic acid in its peptidoglycan layer	1124:1172	Strain HST28T was characterised by the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 : 0 and anteiso-C17 : 0 as major fatty acids (>15 %).
31135331	8	79	theme	new	1647:1649	arg1	species					1651:1657	a new species	1645:1657	a new species named Streptomyces huasconensis sp	1645:1692	Based on the phenotypic and genetic results, strain HST28T (DSM 107268T=CECT 9648T) merits recognition as a new species named Streptomyces huasconensis sp.
31135331	4	80	theme	strain	488:493	arg1	HST28T					495:500	strain HST28T	488:500	strain HST28T	488:500	Optimal growth of strain HST28T was obtained at 28 °C, pH 8-9 and up to 10 % (w/v) NaCl.
31135331	4	81	dep	%	545:545	arg1	10 					542:544	10 	542:544	10 	542:544	Optimal growth of strain HST28T was obtained at 28 °C, pH 8-9 and up to 10 % (w/v) NaCl.
31135331	4	81	dep	%	545:545	arg1	w/v					548:550	w/v	548:550	w/v	548:550	Optimal growth of strain HST28T was obtained at 28 °C, pH 8-9 and up to 10 % (w/v) NaCl.
31135331	3	82	theme	Strain	348:353	arg1	HST28T					355:360	Strain HST28T	348:360	Strain HST28T	348:360	Strain HST28T showed morphological and chemotaxonomic features in line with its classification in the genus Streptomyces.
31135331	3	83	theme	genus	450:454	arg1	Streptomyces					456:467	the genus Streptomyces	446:467	the genus Streptomyces	446:467	Strain HST28T showed morphological and chemotaxonomic features in line with its classification in the genus Streptomyces.
31135331	5	84	theme	kanamyceticus	763:775	arg1	relatives					696:704	its closest relatives	684:704	its closest relatives	684:704	Single (16S rRNA) and multi-locus gene sequence analyses showed that strain HST28T had a distinct phylogenetic position from its closest relatives, the type strains of Steptomyces aureus and Streptomyces kanamyceticus.
31135331	5	84	theme	kanamyceticus	763:775	arg1	strains					716:722	the type strains	707:722	the type strains of Steptomyces aureus and Streptomyces kanamyceticus	707:775	Single (16S rRNA) and multi-locus gene sequence analyses showed that strain HST28T had a distinct phylogenetic position from its closest relatives, the type strains of Steptomyces aureus and Streptomyces kanamyceticus.
31135331	1	85	theme	high	90:93	arg1	saline					104:109	a high altitude saline	88:109	a high altitude saline	88:109	nov., an haloalkalitolerant actinobacterium isolated from a high altitude saline wetland at the Chilean Altiplano.
31135331	8	86	theme	Streptomyces	1665:1676	arg1	sp					1691:1692	Streptomyces huasconensis sp	1665:1692	Streptomyces huasconensis sp	1665:1692	Based on the phenotypic and genetic results, strain HST28T (DSM 107268T=CECT 9648T) merits recognition as a new species named Streptomyces huasconensis sp.
31135331	6	87	theme	%	991:991	arg1	threshold					968:976	the threshold	964:976	the threshold	964:976	Digital DNA-DNA hybridization (23.3 and 31.0 %) and average nucleotide identity (79.3 and 85.6 %) values between strain HST28T and its corresponding relatives mentioned above were below the threshold of 70 and 96 %, respectively, defined for assigning a prokaryotic strains to the same species.
31135331	7	88	theme	Strain	1073:1078	arg1	HST28T					1080:1085	Strain HST28T	1073:1085	Strain HST28T	1073:1085	Strain HST28T was characterised by the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 : 0 and anteiso-C17 : 0 as major fatty acids (>15 %).
31135331	7	89	theme	fatty	1518:1522	arg1	acids					1524:1528	major fatty acids	1512:1528	major fatty acids (>15 %)	1512:1536	Strain HST28T was characterised by the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 : 0 and anteiso-C17 : 0 as major fatty acids (>15 %).
31135331	7	89	theme	fatty	1518:1522	arg1	%					1535:1535	>15 %	1531:1535	>15 %	1531:1535	Strain HST28T was characterised by the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 : 0 and anteiso-C17 : 0 as major fatty acids (>15 %).
31135331	2	90	attach	isolated	172:179	arg1	Salar					190:194	Salar	190:194	Salar	190:194	Streptomyces strain HST28T isolated from the Salar de Huasco, an athalassohaline and poly-extreme high altitude saline wetland located in northern Chile, was the subject of a polyphasic taxonomic study.
31135331	2	90	attach	isolated	172:179	arg2	wetland					264:270	poly-extreme high altitude saline wetland	230:270	poly-extreme high altitude saline wetland	230:270	Streptomyces strain HST28T isolated from the Salar de Huasco, an athalassohaline and poly-extreme high altitude saline wetland located in northern Chile, was the subject of a polyphasic taxonomic study.
31135331	2	90	attach	isolated	172:179	arg1	Huasco					199:204	Huasco	199:204	Huasco	199:204	Streptomyces strain HST28T isolated from the Salar de Huasco, an athalassohaline and poly-extreme high altitude saline wetland located in northern Chile, was the subject of a polyphasic taxonomic study.
31135331	2	90	attach	isolated	172:179	arg2	subject					307:313	the subject	303:313	the subject of a polyphasic taxonomic study	303:345	Streptomyces strain HST28T isolated from the Salar de Huasco, an athalassohaline and poly-extreme high altitude saline wetland located in northern Chile, was the subject of a polyphasic taxonomic study.
31135331	2	90	attach	isolated	172:179	arg2	athalassohaline					210:224	athalassohaline	210:224	athalassohaline	210:224	Streptomyces strain HST28T isolated from the Salar de Huasco, an athalassohaline and poly-extreme high altitude saline wetland located in northern Chile, was the subject of a polyphasic taxonomic study.
31135331	2	90	attach	isolated	172:179	arg2	strain					158:163	Streptomyces strain HST28T	145:170	Streptomyces strain HST28T isolated from the Salar de Huasco	145:204	Streptomyces strain HST28T isolated from the Salar de Huasco, an athalassohaline and poly-extreme high altitude saline wetland located in northern Chile, was the subject of a polyphasic taxonomic study.
31135331	6	91	theme	same	1059:1062	arg1	species					1064:1070	the same species	1055:1070	the same species	1055:1070	Digital DNA-DNA hybridization (23.3 and 31.0 %) and average nucleotide identity (79.3 and 85.6 %) values between strain HST28T and its corresponding relatives mentioned above were below the threshold of 70 and 96 %, respectively, defined for assigning a prokaryotic strains to the same species.
31135331	2	92	theme	taxonomic	331:339	arg1	study					341:345	a polyphasic taxonomic study	318:345	a polyphasic taxonomic study	318:345	Streptomyces strain HST28T isolated from the Salar de Huasco, an athalassohaline and poly-extreme high altitude saline wetland located in northern Chile, was the subject of a polyphasic taxonomic study.
31135331	7	93	from	mannose	1230:1236	arg1	layer					1168:1172	its peptidoglycan layer	1150:1172	its peptidoglycan layer	1150:1172	Strain HST28T was characterised by the presence of ll-diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H6), MK-9(H8) and MK-9(H4) (>20 %) as well as anteiso-C15 : 0 and anteiso-C17 : 0 as major fatty acids (>15 %).
31135331	3	94	from	classification	428:441	arg1	Streptomyces					456:467	the genus Streptomyces	446:467	the genus Streptomyces	446:467	Strain HST28T showed morphological and chemotaxonomic features in line with its classification in the genus Streptomyces.
31135331	6	95	theme	hybridization	794:806	arg1	values					876:881	Digital DNA-DNA hybridization (23.3 and 31.0 %) and average nucleotide identity (79.3 and 85.6 %) values	778:881	Digital DNA-DNA hybridization (23.3 and 31.0 %) and average nucleotide identity (79.3 and 85.6 %) values between strain HST28T and its corresponding relatives mentioned above	778:951	Digital DNA-DNA hybridization (23.3 and 31.0 %) and average nucleotide identity (79.3 and 85.6 %) values between strain HST28T and its corresponding relatives mentioned above were below the threshold of 70 and 96 %, respectively, defined for assigning a prokaryotic strains to the same species.
31135331	6	96	theme	prokaryotic	1032:1042	arg1	strains					1044:1050	a prokaryotic strains	1030:1050	a prokaryotic strains	1030:1050	Digital DNA-DNA hybridization (23.3 and 31.0 %) and average nucleotide identity (79.3 and 85.6 %) values between strain HST28T and its corresponding relatives mentioned above were below the threshold of 70 and 96 %, respectively, defined for assigning a prokaryotic strains to the same species.
31135331	0	97	theme	huasconensis	13:24	arg1	sp					26:27	Streptomyces huasconensis sp	0:27	Streptomyces huasconensis sp.	0:28	Streptomyces huasconensis sp.
29361151	5	0	dep	increased	674:682	arg1	whereas					699:705	whereas	699:705	whereas	699:705	and Enterococcus spp., was increased significantly, whereas probiotics such as Eubacterium rectale, Faecalibacterium prausnitzii and Lactobacillus were dramatically decreased, but BRB anthocyanins supplement could reverse this imbalance in gut microbiota.
29361151	1	1	theme	BRB	166:168	arg1	powder					171:176	Freeze-dried black raspberry (BRB) powder	136:176	Freeze-dried black raspberry (BRB) powder	136:176	Freeze-dried black raspberry (BRB) powder is considered as a potential cancer chemopreventive agent.
29361151	1	1	theme	BRB	166:168	arg1	agent					230:234	a potential cancer chemopreventive agent	195:234	a potential cancer chemopreventive agent	195:234	Freeze-dried black raspberry (BRB) powder is considered as a potential cancer chemopreventive agent.
29361151	8	2	from	status	1365:1370	arg1	inflammation					1332:1343	inflammation	1332:1343	inflammation	1332:1343	Taken together, these results suggested that BRB anthocyanins could modulate the composition of gut commensal microbiota, and changes in inflammation and the methylation status of the SFRP2 gene may play a central role in the chemoprevention of CRC.
29361151	6	3	theme	SFRP2	1015:1019	arg1	expression					1001:1010	increased expression	991:1010	increased expression of SFRP2	991:1019	BRB anthocyanins also caused the demethylation of the SFRP2 gene promoter, resulting in increased expression of SFRP2, both at the mRNA and protein levels.
29361151	3	4	theme	lower	467:471	arg1	multiplicity					479:490	consistently lower tumor multiplicity	454:490	consistently lower tumor multiplicity	454:490	These animals had consistently lower tumor multiplicity compared with AOM/DSS-treated mice not receiving BRB anthocyanins.
29361151	0	5	theme	microbiota	100:109	arg1	modulation					82:91	the modulation	78:91	the modulation of gut microbiota and SFRP2 demethylation	78:133	Chemoprevention of colorectal cancer by black raspberry anthocyanins involved the modulation of gut microbiota and SFRP2 demethylation.
29361151	3	6	theme	tumor	473:477	arg1	multiplicity					479:490	consistently lower tumor multiplicity	454:490	consistently lower tumor multiplicity	454:490	These animals had consistently lower tumor multiplicity compared with AOM/DSS-treated mice not receiving BRB anthocyanins.
29361151	3	7	theme	AOM/DSS-treated	506:520	arg1	mice					522:525	AOM/DSS-treated mice	506:525	AOM/DSS-treated mice not receiving BRB anthocyanins	506:556	These animals had consistently lower tumor multiplicity compared with AOM/DSS-treated mice not receiving BRB anthocyanins.
29361151	0	8	theme	gut	96:98	arg1	microbiota					100:109	gut microbiota	96:109	gut microbiota	96:109	Chemoprevention of colorectal cancer by black raspberry anthocyanins involved the modulation of gut microbiota and SFRP2 demethylation.
29361151	4	9	theme	AOM/DSS-treated	562:576	arg1	mice					578:581	AOM/DSS-treated mice	562:581	AOM/DSS-treated mice	562:581	In AOM/DSS-treated mice, the number of pathogenic bacteria, including Desulfovibrio sp.
29361151	0	10	theme	SFRP2	115:119	arg1	demethylation					121:133	SFRP2 demethylation	115:133	SFRP2 demethylation	115:133	Chemoprevention of colorectal cancer by black raspberry anthocyanins involved the modulation of gut microbiota and SFRP2 demethylation.
29361151	2	11	from	reduction	401:409	arg1	carcinogenesis					420:433	colon carcinogenesis	414:433	colon carcinogenesis	414:433	In this study, we fed azoxymethane (AOM)/dextran sodium sulfate (DSS)-treated C57BL/6J mice with a diet containing BRB anthocyanins for 12 weeks, and this led to a reduction in colon carcinogenesis.
29361151	4	12	theme	pathogenic	598:607	arg1	bacteria					609:616	pathogenic bacteria	598:616	pathogenic bacteria	598:616	In AOM/DSS-treated mice, the number of pathogenic bacteria, including Desulfovibrio sp.
29361151	6	13	theme	protein	1043:1049	arg1	levels					1051:1056	the mRNA and protein levels	1030:1056	the mRNA and protein levels	1030:1056	BRB anthocyanins also caused the demethylation of the SFRP2 gene promoter, resulting in increased expression of SFRP2, both at the mRNA and protein levels.
29361151	6	14	theme	increased	991:999	arg1	expression					1001:1010	increased expression	991:1010	increased expression of SFRP2	991:1019	BRB anthocyanins also caused the demethylation of the SFRP2 gene promoter, resulting in increased expression of SFRP2, both at the mRNA and protein levels.
29361151	3	15	contain	had	450:452	arg1	animals					442:448	These animals	436:448	These animals	436:448	These animals had consistently lower tumor multiplicity compared with AOM/DSS-treated mice not receiving BRB anthocyanins.
29361151	3	15	contain	had	450:452	arg2	multiplicity					479:490	consistently lower tumor multiplicity	454:490	consistently lower tumor multiplicity	454:490	These animals had consistently lower tumor multiplicity compared with AOM/DSS-treated mice not receiving BRB anthocyanins.
29361151	7	16	theme	DNMT31	1097:1102	arg1	levels					1087:1092	the expression levels	1072:1092	the expression levels of DNMT31 and DNMT3B, as well as of p-STAT3	1072:1136	Furthermore, the expression levels of DNMT31 and DNMT3B, as well as of p-STAT3 were downregulated by BRB anthocyanins in these animals.
29361151	2	17	theme	azoxymethane	259:270	arg1	DSS					302:304	DSS	302:304	DSS	302:304	In this study, we fed azoxymethane (AOM)/dextran sodium sulfate (DSS)-treated C57BL/6J mice with a diet containing BRB anthocyanins for 12 weeks, and this led to a reduction in colon carcinogenesis.
29361151	2	17	theme	azoxymethane	259:270	arg1	sulfate					293:299	azoxymethane (AOM)/dextran sodium sulfate	259:299	azoxymethane (AOM)/dextran sodium sulfate (DSS)-treated C57BL/6J mice with a diet containing BRB anthocyanins for 12 weeks	259:380	In this study, we fed azoxymethane (AOM)/dextran sodium sulfate (DSS)-treated C57BL/6J mice with a diet containing BRB anthocyanins for 12 weeks, and this led to a reduction in colon carcinogenesis.
29361151	4	18	theme	Desulfovibrio	629:641	arg1	sp					643:644	Desulfovibrio sp	629:644	Desulfovibrio sp	629:644	In AOM/DSS-treated mice, the number of pathogenic bacteria, including Desulfovibrio sp.
29361151	7	19	theme	expression	1076:1085	arg1	levels					1087:1092	the expression levels	1072:1092	the expression levels of DNMT31 and DNMT3B, as well as of p-STAT3	1072:1136	Furthermore, the expression levels of DNMT31 and DNMT3B, as well as of p-STAT3 were downregulated by BRB anthocyanins in these animals.
29361151	3	20	theme	BRB	541:543	arg1	anthocyanins					545:556	BRB anthocyanins	541:556	BRB anthocyanins	541:556	These animals had consistently lower tumor multiplicity compared with AOM/DSS-treated mice not receiving BRB anthocyanins.
29361151	6	21	theme	BRB	903:905	arg1	anthocyanins					907:918	BRB anthocyanins	903:918	BRB anthocyanins	903:918	BRB anthocyanins also caused the demethylation of the SFRP2 gene promoter, resulting in increased expression of SFRP2, both at the mRNA and protein levels.
29361151	2	22	theme	BRB	352:354	arg1	anthocyanins					356:367	BRB anthocyanins	352:367	BRB anthocyanins	352:367	In this study, we fed azoxymethane (AOM)/dextran sodium sulfate (DSS)-treated C57BL/6J mice with a diet containing BRB anthocyanins for 12 weeks, and this led to a reduction in colon carcinogenesis.
29361151	7	23	theme	BRB	1160:1162	arg1	anthocyanins					1164:1175	BRB anthocyanins	1160:1175	BRB anthocyanins	1160:1175	Furthermore, the expression levels of DNMT31 and DNMT3B, as well as of p-STAT3 were downregulated by BRB anthocyanins in these animals.
29361151	2	24	theme	sulfate	293:299	arg1	mice					324:327	azoxymethane (AOM)/dextran sodium sulfate (DSS)-treated C57BL/6J mice	259:327	azoxymethane (AOM)/dextran sodium sulfate (DSS)-treated C57BL/6J mice with a diet containing BRB anthocyanins for 12 weeks	259:380	In this study, we fed azoxymethane (AOM)/dextran sodium sulfate (DSS)-treated C57BL/6J mice with a diet containing BRB anthocyanins for 12 weeks, and this led to a reduction in colon carcinogenesis.
29361151	8	25	theme	BRB	1240:1242	arg1	anthocyanins					1244:1255	BRB anthocyanins	1240:1255	BRB anthocyanins	1240:1255	Taken together, these results suggested that BRB anthocyanins could modulate the composition of gut commensal microbiota, and changes in inflammation and the methylation status of the SFRP2 gene may play a central role in the chemoprevention of CRC.
29361151	5	26	theme	gut	887:889	arg1	microbiota					891:900	gut microbiota	887:900	gut microbiota	887:900	and Enterococcus spp., was increased significantly, whereas probiotics such as Eubacterium rectale, Faecalibacterium prausnitzii and Lactobacillus were dramatically decreased, but BRB anthocyanins supplement could reverse this imbalance in gut microbiota.
29361151	0	27	theme	cancer	30:35	arg1	Chemoprevention					0:14	Chemoprevention	0:14	Chemoprevention of colorectal cancer by black raspberry anthocyanins	0:67	Chemoprevention of colorectal cancer by black raspberry anthocyanins involved the modulation of gut microbiota and SFRP2 demethylation.
29361151	8	28	theme	gene	1385:1388	arg1	changes					1321:1327	changes	1321:1327	changes in inflammation	1321:1343	Taken together, these results suggested that BRB anthocyanins could modulate the composition of gut commensal microbiota, and changes in inflammation and the methylation status of the SFRP2 gene may play a central role in the chemoprevention of CRC.
29361151	8	28	theme	gene	1385:1388	arg1	status					1365:1370	the methylation status	1349:1370	the methylation status of the SFRP2 gene	1349:1388	Taken together, these results suggested that BRB anthocyanins could modulate the composition of gut commensal microbiota, and changes in inflammation and the methylation status of the SFRP2 gene may play a central role in the chemoprevention of CRC.
29361151	2	29	theme	sodium	286:291	arg1	DSS					302:304	DSS	302:304	DSS	302:304	In this study, we fed azoxymethane (AOM)/dextran sodium sulfate (DSS)-treated C57BL/6J mice with a diet containing BRB anthocyanins for 12 weeks, and this led to a reduction in colon carcinogenesis.
29361151	2	29	theme	sodium	286:291	arg1	sulfate					293:299	azoxymethane (AOM)/dextran sodium sulfate	259:299	azoxymethane (AOM)/dextran sodium sulfate (DSS)-treated C57BL/6J mice with a diet containing BRB anthocyanins for 12 weeks	259:380	In this study, we fed azoxymethane (AOM)/dextran sodium sulfate (DSS)-treated C57BL/6J mice with a diet containing BRB anthocyanins for 12 weeks, and this led to a reduction in colon carcinogenesis.
29361151	4	30	theme	bacteria	609:616	arg1	number					588:593	the number	584:593	the number of pathogenic bacteria	584:616	In AOM/DSS-treated mice, the number of pathogenic bacteria, including Desulfovibrio sp.
29361151	0	31	theme	colorectal	19:28	arg1	cancer					30:35	colorectal cancer	19:35	colorectal cancer	19:35	Chemoprevention of colorectal cancer by black raspberry anthocyanins involved the modulation of gut microbiota and SFRP2 demethylation.
29361151	0	32	theme	demethylation	121:133	arg1	modulation					82:91	the modulation	78:91	the modulation of gut microbiota and SFRP2 demethylation	78:133	Chemoprevention of colorectal cancer by black raspberry anthocyanins involved the modulation of gut microbiota and SFRP2 demethylation.
29361151	2	33	contain	containing	341:350	arg2	anthocyanins					356:367	BRB anthocyanins	352:367	BRB anthocyanins	352:367	In this study, we fed azoxymethane (AOM)/dextran sodium sulfate (DSS)-treated C57BL/6J mice with a diet containing BRB anthocyanins for 12 weeks, and this led to a reduction in colon carcinogenesis.
29361151	2	33	contain	containing	341:350	arg1	diet					336:339	a diet	334:339	a diet containing BRB anthocyanins for 12 weeks	334:380	In this study, we fed azoxymethane (AOM)/dextran sodium sulfate (DSS)-treated C57BL/6J mice with a diet containing BRB anthocyanins for 12 weeks, and this led to a reduction in colon carcinogenesis.
29361151	2	34	theme	/dextran	277:284	arg1	DSS					302:304	DSS	302:304	DSS	302:304	In this study, we fed azoxymethane (AOM)/dextran sodium sulfate (DSS)-treated C57BL/6J mice with a diet containing BRB anthocyanins for 12 weeks, and this led to a reduction in colon carcinogenesis.
29361151	2	34	theme	/dextran	277:284	arg1	sulfate					293:299	azoxymethane (AOM)/dextran sodium sulfate	259:299	azoxymethane (AOM)/dextran sodium sulfate (DSS)-treated C57BL/6J mice with a diet containing BRB anthocyanins for 12 weeks	259:380	In this study, we fed azoxymethane (AOM)/dextran sodium sulfate (DSS)-treated C57BL/6J mice with a diet containing BRB anthocyanins for 12 weeks, and this led to a reduction in colon carcinogenesis.
29361151	1	35	theme	potential	197:205	arg1	agent					230:234	a potential cancer chemopreventive agent	195:234	a potential cancer chemopreventive agent	195:234	Freeze-dried black raspberry (BRB) powder is considered as a potential cancer chemopreventive agent.
29361151	1	35	theme	potential	197:205	arg1	powder					171:176	Freeze-dried black raspberry (BRB) powder	136:176	Freeze-dried black raspberry (BRB) powder	136:176	Freeze-dried black raspberry (BRB) powder is considered as a potential cancer chemopreventive agent.
29361151	0	36	theme	black	40:44	arg1	anthocyanins					56:67	black raspberry anthocyanins	40:67	black raspberry anthocyanins	40:67	Chemoprevention of colorectal cancer by black raspberry anthocyanins involved the modulation of gut microbiota and SFRP2 demethylation.
29361151	6	37	theme	promoter	968:975	arg1	demethylation					936:948	the demethylation	932:948	the demethylation of the SFRP2 gene promoter	932:975	BRB anthocyanins also caused the demethylation of the SFRP2 gene promoter, resulting in increased expression of SFRP2, both at the mRNA and protein levels.
29361151	8	38	theme	SFRP2	1379:1383	arg1	gene					1385:1388	the SFRP2 gene	1375:1388	the SFRP2 gene	1375:1388	Taken together, these results suggested that BRB anthocyanins could modulate the composition of gut commensal microbiota, and changes in inflammation and the methylation status of the SFRP2 gene may play a central role in the chemoprevention of CRC.
29361151	1	39	theme	cancer	207:212	arg1	agent					230:234	a potential cancer chemopreventive agent	195:234	a potential cancer chemopreventive agent	195:234	Freeze-dried black raspberry (BRB) powder is considered as a potential cancer chemopreventive agent.
29361151	1	39	theme	cancer	207:212	arg1	powder					171:176	Freeze-dried black raspberry (BRB) powder	136:176	Freeze-dried black raspberry (BRB) powder	136:176	Freeze-dried black raspberry (BRB) powder is considered as a potential cancer chemopreventive agent.
29361151	2	40	theme	colon	414:418	arg1	carcinogenesis					420:433	colon carcinogenesis	414:433	colon carcinogenesis	414:433	In this study, we fed azoxymethane (AOM)/dextran sodium sulfate (DSS)-treated C57BL/6J mice with a diet containing BRB anthocyanins for 12 weeks, and this led to a reduction in colon carcinogenesis.
29361151	6	41	theme	gene	963:966	arg1	promoter					968:975	the SFRP2 gene promoter	953:975	the SFRP2 gene promoter	953:975	BRB anthocyanins also caused the demethylation of the SFRP2 gene promoter, resulting in increased expression of SFRP2, both at the mRNA and protein levels.
29361151	7	42	theme	p-STAT3	1130:1136	arg1	levels					1087:1092	the expression levels	1072:1092	the expression levels of DNMT31 and DNMT3B, as well as of p-STAT3	1072:1136	Furthermore, the expression levels of DNMT31 and DNMT3B, as well as of p-STAT3 were downregulated by BRB anthocyanins in these animals.
29361151	1	43	theme	chemopreventive	214:228	arg1	agent					230:234	a potential cancer chemopreventive agent	195:234	a potential cancer chemopreventive agent	195:234	Freeze-dried black raspberry (BRB) powder is considered as a potential cancer chemopreventive agent.
29361151	1	43	theme	chemopreventive	214:228	arg1	powder					171:176	Freeze-dried black raspberry (BRB) powder	136:176	Freeze-dried black raspberry (BRB) powder	136:176	Freeze-dried black raspberry (BRB) powder is considered as a potential cancer chemopreventive agent.
29361151	6	44	theme	SFRP2	957:961	arg1	promoter					968:975	the SFRP2 gene promoter	953:975	the SFRP2 gene promoter	953:975	BRB anthocyanins also caused the demethylation of the SFRP2 gene promoter, resulting in increased expression of SFRP2, both at the mRNA and protein levels.
29361151	8	45	theme	methylation	1353:1363	arg1	status					1365:1370	the methylation status	1349:1370	the methylation status of the SFRP2 gene	1349:1388	Taken together, these results suggested that BRB anthocyanins could modulate the composition of gut commensal microbiota, and changes in inflammation and the methylation status of the SFRP2 gene may play a central role in the chemoprevention of CRC.
29361151	2	46	theme	C57BL/6J	315:322	arg1	mice					324:327	azoxymethane (AOM)/dextran sodium sulfate (DSS)-treated C57BL/6J mice	259:327	azoxymethane (AOM)/dextran sodium sulfate (DSS)-treated C57BL/6J mice with a diet containing BRB anthocyanins for 12 weeks	259:380	In this study, we fed azoxymethane (AOM)/dextran sodium sulfate (DSS)-treated C57BL/6J mice with a diet containing BRB anthocyanins for 12 weeks, and this led to a reduction in colon carcinogenesis.
29361151	0	47	theme	raspberry	46:54	arg1	anthocyanins					56:67	black raspberry anthocyanins	40:67	black raspberry anthocyanins	40:67	Chemoprevention of colorectal cancer by black raspberry anthocyanins involved the modulation of gut microbiota and SFRP2 demethylation.
29361151	8	48	theme	CRC	1440:1442	arg1	chemoprevention					1421:1435	the chemoprevention	1417:1435	the chemoprevention of CRC	1417:1442	Taken together, these results suggested that BRB anthocyanins could modulate the composition of gut commensal microbiota, and changes in inflammation and the methylation status of the SFRP2 gene may play a central role in the chemoprevention of CRC.
29361151	2	49	theme	-treated	306:313	arg1	mice					324:327	azoxymethane (AOM)/dextran sodium sulfate (DSS)-treated C57BL/6J mice	259:327	azoxymethane (AOM)/dextran sodium sulfate (DSS)-treated C57BL/6J mice with a diet containing BRB anthocyanins for 12 weeks	259:380	In this study, we fed azoxymethane (AOM)/dextran sodium sulfate (DSS)-treated C57BL/6J mice with a diet containing BRB anthocyanins for 12 weeks, and this led to a reduction in colon carcinogenesis.
29361151	8	50	theme	central	1401:1407	arg1	role					1409:1412	a central role	1399:1412	a central role	1399:1412	Taken together, these results suggested that BRB anthocyanins could modulate the composition of gut commensal microbiota, and changes in inflammation and the methylation status of the SFRP2 gene may play a central role in the chemoprevention of CRC.
29361151	2	51	with	mice	324:327	arg1	diet					336:339	a diet	334:339	a diet containing BRB anthocyanins for 12 weeks	334:380	In this study, we fed azoxymethane (AOM)/dextran sodium sulfate (DSS)-treated C57BL/6J mice with a diet containing BRB anthocyanins for 12 weeks, and this led to a reduction in colon carcinogenesis.
29361151	1	52	theme	Freeze-dried	136:147	arg1	powder					171:176	Freeze-dried black raspberry (BRB) powder	136:176	Freeze-dried black raspberry (BRB) powder	136:176	Freeze-dried black raspberry (BRB) powder is considered as a potential cancer chemopreventive agent.
29361151	1	52	theme	Freeze-dried	136:147	arg1	agent					230:234	a potential cancer chemopreventive agent	195:234	a potential cancer chemopreventive agent	195:234	Freeze-dried black raspberry (BRB) powder is considered as a potential cancer chemopreventive agent.
29361151	8	53	theme	microbiota	1305:1314	arg1	composition					1276:1286	the composition	1272:1286	the composition of gut commensal microbiota	1272:1314	Taken together, these results suggested that BRB anthocyanins could modulate the composition of gut commensal microbiota, and changes in inflammation and the methylation status of the SFRP2 gene may play a central role in the chemoprevention of CRC.
29361151	1	54	theme	black	149:153	arg1	powder					171:176	Freeze-dried black raspberry (BRB) powder	136:176	Freeze-dried black raspberry (BRB) powder	136:176	Freeze-dried black raspberry (BRB) powder is considered as a potential cancer chemopreventive agent.
29361151	1	54	theme	black	149:153	arg1	agent					230:234	a potential cancer chemopreventive agent	195:234	a potential cancer chemopreventive agent	195:234	Freeze-dried black raspberry (BRB) powder is considered as a potential cancer chemopreventive agent.
29361151	8	55	theme	gut	1291:1293	arg1	microbiota					1305:1314	gut commensal microbiota	1291:1314	gut commensal microbiota	1291:1314	Taken together, these results suggested that BRB anthocyanins could modulate the composition of gut commensal microbiota, and changes in inflammation and the methylation status of the SFRP2 gene may play a central role in the chemoprevention of CRC.
29361151	6	56	theme	mRNA	1034:1037	arg1	levels					1051:1056	the mRNA and protein levels	1030:1056	the mRNA and protein levels	1030:1056	BRB anthocyanins also caused the demethylation of the SFRP2 gene promoter, resulting in increased expression of SFRP2, both at the mRNA and protein levels.
29361151	5	57	dep	anthocyanins	831:842	arg1	supplement					844:853	supplement	844:853	BRB anthocyanins supplement	827:853	and Enterococcus spp., was increased significantly, whereas probiotics such as Eubacterium rectale, Faecalibacterium prausnitzii and Lactobacillus were dramatically decreased, but BRB anthocyanins supplement could reverse this imbalance in gut microbiota.
29361151	1	58	theme	raspberry	155:163	arg1	powder					171:176	Freeze-dried black raspberry (BRB) powder	136:176	Freeze-dried black raspberry (BRB) powder	136:176	Freeze-dried black raspberry (BRB) powder is considered as a potential cancer chemopreventive agent.
29361151	1	58	theme	raspberry	155:163	arg1	agent					230:234	a potential cancer chemopreventive agent	195:234	a potential cancer chemopreventive agent	195:234	Freeze-dried black raspberry (BRB) powder is considered as a potential cancer chemopreventive agent.
29361151	8	59	from	changes	1321:1327	arg1	inflammation					1332:1343	inflammation	1332:1343	inflammation	1332:1343	Taken together, these results suggested that BRB anthocyanins could modulate the composition of gut commensal microbiota, and changes in inflammation and the methylation status of the SFRP2 gene may play a central role in the chemoprevention of CRC.
29361151	6	60	dep	expression	1001:1010	arg1	both					1022:1025	both	1022:1025	both	1022:1025	BRB anthocyanins also caused the demethylation of the SFRP2 gene promoter, resulting in increased expression of SFRP2, both at the mRNA and protein levels.
29361151	5	61	theme	BRB	827:829	arg1	anthocyanins					831:842	BRB anthocyanins	827:842	BRB anthocyanins supplement	827:853	and Enterococcus spp., was increased significantly, whereas probiotics such as Eubacterium rectale, Faecalibacterium prausnitzii and Lactobacillus were dramatically decreased, but BRB anthocyanins supplement could reverse this imbalance in gut microbiota.
29361151	8	62	theme	commensal	1295:1303	arg1	microbiota					1305:1314	gut commensal microbiota	1291:1314	gut commensal microbiota	1291:1314	Taken together, these results suggested that BRB anthocyanins could modulate the composition of gut commensal microbiota, and changes in inflammation and the methylation status of the SFRP2 gene may play a central role in the chemoprevention of CRC.
31483753	3	0	theme	Strain	211:216	arg1	A59T					218:221	Strain A59T	211:221	Strain A59T	211:221	Strain A59T is a Gram-stain-positive, facultative anaerobic, endospore-forming and rod-shaped bacterium.
31483753	3	0	theme	Strain	211:216	arg1	bacterium					305:313	a Gram-stain-positive, facultative anaerobic, endospore-forming and rod-shaped bacterium	226:313	a Gram-stain-positive, facultative anaerobic, endospore-forming and rod-shaped bacterium	226:313	Strain A59T is a Gram-stain-positive, facultative anaerobic, endospore-forming and rod-shaped bacterium.
31483753	4	1	theme	growth	328:333	arg1	30 °C					350:354	30 °C	350:354	30 °C (range, 28-37 °C)	350:372	Its optimal growth conditions are 30 °C (range, 28-37 °C), pH 7 (range, pH 5-10) and it tolerates up to 7 % of NaCl (range, 2-7 %).
31483753	4	1	theme	growth	328:333	arg1	conditions					335:344	Its optimal growth conditions	316:344	Its optimal growth conditions	316:344	Its optimal growth conditions are 30 °C (range, 28-37 °C), pH 7 (range, pH 5-10) and it tolerates up to 7 % of NaCl (range, 2-7 %).
31483753	2	2	theme	bacterial	115:123	arg1	strain					125:130	A xylanolytic bacterial strain	101:130	A xylanolytic bacterial strain	101:130	A xylanolytic bacterial strain, named A59T, was isolated from a forest soil consortium in southern Argentina.
31483753	7	3	theme	RNA	731:733	arg1	analysis					749:756	16S RNA gene sequence analysis	727:756	16S RNA gene sequence analysis	727:756	According to 16S RNA gene sequence analysis, the isolate is phylogenetically placed in the same cluster as Paenibacillus taichungensis BCRC 17757T (99.7 % nucleotide sequence identity) and Paenibacillus pabuli NBRC 13638T (99.1 %) and is closely related to Paenibacillus tundrae A10bT (98.8 %).
31483753	2	4	from	consortium	177:186	arg1	Argentina					200:208	southern Argentina	191:208	southern Argentina	191:208	A xylanolytic bacterial strain, named A59T, was isolated from a forest soil consortium in southern Argentina.
31483753	6	5	theme	C16 	697:700	arg1	C16 					697:700	C16 	697:700	C16 	697:700	It contains menaquinone MK-7 as the predominant isoprenoid quinone and the major fatty acid is anteiso-C15 : 0 (35.1 %), with a moderate amount of C16 : 0 (6.9 %).
31483753	6	5	theme	C16 	697:700	arg1	amount					687:692	a moderate amount	676:692	a moderate amount of C16 	676:700	It contains menaquinone MK-7 as the predominant isoprenoid quinone and the major fatty acid is anteiso-C15 : 0 (35.1 %), with a moderate amount of C16 : 0 (6.9 %).
31483753	9	6	theme	type	1243:1246	arg1	strains					1248:1254	related type strains	1235:1254	related type strains	1235:1254	Furthermore, the results of whole genome average nucleotide identity analysis (gANI) with related type strains was lower than 91.10 % and the digital DNA-DNA hybridization value was lower than 44.30 %, which are below the threshold values for separating two species.
31483753	3	7	theme	Gram-stain-positive	228:246	arg1	A59T					218:221	Strain A59T	211:221	Strain A59T	211:221	Strain A59T is a Gram-stain-positive, facultative anaerobic, endospore-forming and rod-shaped bacterium.
31483753	3	7	theme	Gram-stain-positive	228:246	arg1	bacterium					305:313	a Gram-stain-positive, facultative anaerobic, endospore-forming and rod-shaped bacterium	226:313	a Gram-stain-positive, facultative anaerobic, endospore-forming and rod-shaped bacterium	226:313	Strain A59T is a Gram-stain-positive, facultative anaerobic, endospore-forming and rod-shaped bacterium.
31483753	11	8	theme	genus	1557:1561	arg1	species					1542:1548	a new species	1536:1548	a new species of the genus Paenibacillus	1536:1575	On the basis of these results, A59T represents a new species of the genus Paenibacillus, and we propose the name Paenibacillusxylanivorans sp.
31483753	5	9	theme	meso-diaminopimelic	507:525	arg1	acid					527:530	meso-diaminopimelic acid	507:530	meso-diaminopimelic acid in the cell wall	507:547	Chemotaxonomic analysis revealed that strain A59Tpossesses meso-diaminopimelic acid in the cell wall.
31483753	5	10	from	acid	527:530	arg1	wall					544:547	the cell wall	535:547	the cell wall	535:547	Chemotaxonomic analysis revealed that strain A59Tpossesses meso-diaminopimelic acid in the cell wall.
31483753	10	11	theme	DNA	1416:1418	arg1	%					1458:1458	46.09 mol%	1449:1458	46.09 mol%	1449:1458	The DNA G+C content was estimated as 46.09 mol%, based on genome sequencing.
31483753	10	11	theme	DNA	1416:1418	arg1	content					1424:1430	The DNA G+C content	1412:1430	The DNA G+C content	1412:1430	The DNA G+C content was estimated as 46.09 mol%, based on genome sequencing.
31483753	7	12	theme	NBRC	924:927	arg1	isolate					763:769	the isolate	759:769	the isolate	759:769	According to 16S RNA gene sequence analysis, the isolate is phylogenetically placed in the same cluster as Paenibacillus taichungensis BCRC 17757T (99.7 % nucleotide sequence identity) and Paenibacillus pabuli NBRC 13638T (99.1 %) and is closely related to Paenibacillus tundrae A10bT (98.8 %).
31483753	7	12	theme	NBRC	924:927	arg1	13638T					929:934	Paenibacillus pabuli NBRC 13638T	903:934	Paenibacillus pabuli NBRC 13638T (99.1 %)	903:943	According to 16S RNA gene sequence analysis, the isolate is phylogenetically placed in the same cluster as Paenibacillus taichungensis BCRC 17757T (99.7 % nucleotide sequence identity) and Paenibacillus pabuli NBRC 13638T (99.1 %) and is closely related to Paenibacillus tundrae A10bT (98.8 %).
31483753	7	12	theme	NBRC	924:927	arg1	%					942:942	99.1 %	937:942	99.1 %	937:942	According to 16S RNA gene sequence analysis, the isolate is phylogenetically placed in the same cluster as Paenibacillus taichungensis BCRC 17757T (99.7 % nucleotide sequence identity) and Paenibacillus pabuli NBRC 13638T (99.1 %) and is closely related to Paenibacillus tundrae A10bT (98.8 %).
31483753	7	13	theme	nucleotide	869:878	arg1	17757T					854:859	Paenibacillus taichungensis BCRC 17757T	821:859	Paenibacillus taichungensis BCRC 17757T (99.7 % nucleotide sequence identity)	821:897	According to 16S RNA gene sequence analysis, the isolate is phylogenetically placed in the same cluster as Paenibacillus taichungensis BCRC 17757T (99.7 % nucleotide sequence identity) and Paenibacillus pabuli NBRC 13638T (99.1 %) and is closely related to Paenibacillus tundrae A10bT (98.8 %).
31483753	7	13	theme	nucleotide	869:878	arg1	identity					889:896	99.7 % nucleotide sequence identity	862:896	99.7 % nucleotide sequence identity	862:896	According to 16S RNA gene sequence analysis, the isolate is phylogenetically placed in the same cluster as Paenibacillus taichungensis BCRC 17757T (99.7 % nucleotide sequence identity) and Paenibacillus pabuli NBRC 13638T (99.1 %) and is closely related to Paenibacillus tundrae A10bT (98.8 %).
31483753	8	14	theme	housekeeping	1052:1063	arg1	gene					1070:1073	the housekeeping gyrB gene	1048:1073	the housekeeping gyrB gene	1048:1073	However, phylogenetic studies based on the housekeeping gyrB gene placed A59T in a separate branch from all other related type strains.
31483753	7	15	theme	Paenibacillus	903:915	arg1	isolate					763:769	the isolate	759:769	the isolate	759:769	According to 16S RNA gene sequence analysis, the isolate is phylogenetically placed in the same cluster as Paenibacillus taichungensis BCRC 17757T (99.7 % nucleotide sequence identity) and Paenibacillus pabuli NBRC 13638T (99.1 %) and is closely related to Paenibacillus tundrae A10bT (98.8 %).
31483753	7	15	theme	Paenibacillus	903:915	arg1	13638T					929:934	Paenibacillus pabuli NBRC 13638T	903:934	Paenibacillus pabuli NBRC 13638T (99.1 %)	903:943	According to 16S RNA gene sequence analysis, the isolate is phylogenetically placed in the same cluster as Paenibacillus taichungensis BCRC 17757T (99.7 % nucleotide sequence identity) and Paenibacillus pabuli NBRC 13638T (99.1 %) and is closely related to Paenibacillus tundrae A10bT (98.8 %).
31483753	7	15	theme	Paenibacillus	903:915	arg1	%					942:942	99.1 %	937:942	99.1 %	937:942	According to 16S RNA gene sequence analysis, the isolate is phylogenetically placed in the same cluster as Paenibacillus taichungensis BCRC 17757T (99.7 % nucleotide sequence identity) and Paenibacillus pabuli NBRC 13638T (99.1 %) and is closely related to Paenibacillus tundrae A10bT (98.8 %).
31483753	7	16	theme	99.7 	862:866	arg1	17757T					854:859	Paenibacillus taichungensis BCRC 17757T	821:859	Paenibacillus taichungensis BCRC 17757T (99.7 % nucleotide sequence identity)	821:897	According to 16S RNA gene sequence analysis, the isolate is phylogenetically placed in the same cluster as Paenibacillus taichungensis BCRC 17757T (99.7 % nucleotide sequence identity) and Paenibacillus pabuli NBRC 13638T (99.1 %) and is closely related to Paenibacillus tundrae A10bT (98.8 %).
31483753	7	16	theme	99.7 	862:866	arg1	identity					889:896	99.7 % nucleotide sequence identity	862:896	99.7 % nucleotide sequence identity	862:896	According to 16S RNA gene sequence analysis, the isolate is phylogenetically placed in the same cluster as Paenibacillus taichungensis BCRC 17757T (99.7 % nucleotide sequence identity) and Paenibacillus pabuli NBRC 13638T (99.1 %) and is closely related to Paenibacillus tundrae A10bT (98.8 %).
31483753	9	17	theme	DNA-DNA	1295:1301	arg1	hybridization					1303:1315	digital DNA-DNA hybridization	1287:1315	the digital DNA-DNA hybridization value	1283:1321	Furthermore, the results of whole genome average nucleotide identity analysis (gANI) with related type strains was lower than 91.10 % and the digital DNA-DNA hybridization value was lower than 44.30 %, which are below the threshold values for separating two species.
31483753	6	18	contain	contains	553:560	arg1	It					550:551	It	550:551	It	550:551	It contains menaquinone MK-7 as the predominant isoprenoid quinone and the major fatty acid is anteiso-C15 : 0 (35.1 %), with a moderate amount of C16 : 0 (6.9 %).
31483753	6	18	contain	contains	553:560	arg2	quinone					609:615	the predominant isoprenoid quinone	582:615	the predominant isoprenoid quinone	582:615	It contains menaquinone MK-7 as the predominant isoprenoid quinone and the major fatty acid is anteiso-C15 : 0 (35.1 %), with a moderate amount of C16 : 0 (6.9 %).
31483753	6	18	contain	contains	553:560	arg2	MK-7					574:577	menaquinone MK-7	562:577	menaquinone MK-7	562:577	It contains menaquinone MK-7 as the predominant isoprenoid quinone and the major fatty acid is anteiso-C15 : 0 (35.1 %), with a moderate amount of C16 : 0 (6.9 %).
31483753	8	19	theme	related	1123:1129	arg1	strains					1136:1142	all other related type strains	1113:1142	all other related type strains	1113:1142	However, phylogenetic studies based on the housekeeping gyrB gene placed A59T in a separate branch from all other related type strains.
31483753	13	20	theme	=DSM	1662:1665	arg1	A59T					1656:1659	A59T	1656:1659	A59T (=DSM 107920T=NCIMB 15123T)	1656:1687	The type strain is A59T (=DSM 107920T=NCIMB 15123T).
31483753	13	20	theme	=DSM	1662:1665	arg1	15123T					1681:1686	=DSM 107920T=NCIMB 15123T	1662:1686	=DSM 107920T=NCIMB 15123T	1662:1686	The type strain is A59T (=DSM 107920T=NCIMB 15123T).
31483753	7	21	theme	taichungensis	835:847	arg1	17757T					854:859	Paenibacillus taichungensis BCRC 17757T	821:859	Paenibacillus taichungensis BCRC 17757T (99.7 % nucleotide sequence identity)	821:897	According to 16S RNA gene sequence analysis, the isolate is phylogenetically placed in the same cluster as Paenibacillus taichungensis BCRC 17757T (99.7 % nucleotide sequence identity) and Paenibacillus pabuli NBRC 13638T (99.1 %) and is closely related to Paenibacillus tundrae A10bT (98.8 %).
31483753	7	21	theme	taichungensis	835:847	arg1	identity					889:896	99.7 % nucleotide sequence identity	862:896	99.7 % nucleotide sequence identity	862:896	According to 16S RNA gene sequence analysis, the isolate is phylogenetically placed in the same cluster as Paenibacillus taichungensis BCRC 17757T (99.7 % nucleotide sequence identity) and Paenibacillus pabuli NBRC 13638T (99.1 %) and is closely related to Paenibacillus tundrae A10bT (98.8 %).
31483753	7	21	theme	taichungensis	835:847	arg1	isolate					763:769	the isolate	759:769	the isolate	759:769	According to 16S RNA gene sequence analysis, the isolate is phylogenetically placed in the same cluster as Paenibacillus taichungensis BCRC 17757T (99.7 % nucleotide sequence identity) and Paenibacillus pabuli NBRC 13638T (99.1 %) and is closely related to Paenibacillus tundrae A10bT (98.8 %).
31483753	4	22	theme	NaCl	427:430	arg1	%					422:422	up to 7 %	414:422	up to 7 % of NaCl (range, 2-7 %)	414:445	Its optimal growth conditions are 30 °C (range, 28-37 °C), pH 7 (range, pH 5-10) and it tolerates up to 7 % of NaCl (range, 2-7 %).
31483753	4	22	theme	NaCl	427:430	arg1	NaCl					427:430	NaCl	427:430	NaCl	427:430	Its optimal growth conditions are 30 °C (range, 28-37 °C), pH 7 (range, pH 5-10) and it tolerates up to 7 % of NaCl (range, 2-7 %).
31483753	11	23	theme	new	1538:1540	arg1	species					1542:1548	a new species	1536:1548	a new species of the genus Paenibacillus	1536:1575	On the basis of these results, A59T represents a new species of the genus Paenibacillus, and we propose the name Paenibacillusxylanivorans sp.
31483753	4	24	dep	pH	375:376	arg1	pH					388:389	pH 5-10	388:394	pH 5-10	388:394	Its optimal growth conditions are 30 °C (range, 28-37 °C), pH 7 (range, pH 5-10) and it tolerates up to 7 % of NaCl (range, 2-7 %).
31483753	4	24	dep	pH	375:376	arg1	range					381:385	range	381:385	range	381:385	Its optimal growth conditions are 30 °C (range, 28-37 °C), pH 7 (range, pH 5-10) and it tolerates up to 7 % of NaCl (range, 2-7 %).
31483753	5	25	theme	cell	539:542	arg1	wall					544:547	the cell wall	535:547	the cell wall	535:547	Chemotaxonomic analysis revealed that strain A59Tpossesses meso-diaminopimelic acid in the cell wall.
31483753	7	26	theme	same	805:808	arg1	cluster					810:816	the same cluster	801:816	the same cluster	801:816	According to 16S RNA gene sequence analysis, the isolate is phylogenetically placed in the same cluster as Paenibacillus taichungensis BCRC 17757T (99.7 % nucleotide sequence identity) and Paenibacillus pabuli NBRC 13638T (99.1 %) and is closely related to Paenibacillus tundrae A10bT (98.8 %).
31483753	6	27	theme	major	625:629	arg1	anteiso-C15 					645:656	anteiso-C15 	645:656	anteiso-C15 	645:656	It contains menaquinone MK-7 as the predominant isoprenoid quinone and the major fatty acid is anteiso-C15 : 0 (35.1 %), with a moderate amount of C16 : 0 (6.9 %).
31483753	6	27	theme	major	625:629	arg1	acid					637:640	the major fatty acid	621:640	the major fatty acid	621:640	It contains menaquinone MK-7 as the predominant isoprenoid quinone and the major fatty acid is anteiso-C15 : 0 (35.1 %), with a moderate amount of C16 : 0 (6.9 %).
31483753	0	28	theme	Paenibacillus	0:12	arg1	xylanivorans					14:25	Paenibacillus xylanivorans	0:25	Paenibacillus xylanivorans	0:25	Paenibacillus xylanivorans sp.
31483753	8	29	from	strains	1136:1142	arg1	branch					1101:1106	a separate branch	1090:1106	a separate branch from all other related type strains	1090:1142	However, phylogenetic studies based on the housekeeping gyrB gene placed A59T in a separate branch from all other related type strains.
31483753	4	30	dep	7 	420:421	arg1	to					417:418	to	417:418	to	417:418	Its optimal growth conditions are 30 °C (range, 28-37 °C), pH 7 (range, pH 5-10) and it tolerates up to 7 % of NaCl (range, 2-7 %).
31483753	6	31	theme	isoprenoid	598:607	arg1	quinone					609:615	the predominant isoprenoid quinone	582:615	the predominant isoprenoid quinone	582:615	It contains menaquinone MK-7 as the predominant isoprenoid quinone and the major fatty acid is anteiso-C15 : 0 (35.1 %), with a moderate amount of C16 : 0 (6.9 %).
31483753	6	31	theme	isoprenoid	598:607	arg1	MK-7					574:577	menaquinone MK-7	562:577	menaquinone MK-7	562:577	It contains menaquinone MK-7 as the predominant isoprenoid quinone and the major fatty acid is anteiso-C15 : 0 (35.1 %), with a moderate amount of C16 : 0 (6.9 %).
31483753	9	32	theme	whole	1173:1177	arg1	gANI					1224:1227	whole genome average nucleotide identity analysis (gANI)	1173:1228	whole genome average nucleotide identity analysis (gANI) with related type strains	1173:1254	Furthermore, the results of whole genome average nucleotide identity analysis (gANI) with related type strains was lower than 91.10 % and the digital DNA-DNA hybridization value was lower than 44.30 %, which are below the threshold values for separating two species.
31483753	11	33	theme	Paenibacillusxylanivorans	1602:1626	arg1	sp					1628:1629	the name Paenibacillusxylanivorans sp	1593:1629	the name Paenibacillusxylanivorans sp	1593:1629	On the basis of these results, A59T represents a new species of the genus Paenibacillus, and we propose the name Paenibacillusxylanivorans sp.
31483753	7	34	theme	tundrae	985:991	arg1	A10bT					993:997	Paenibacillus tundrae A10bT	971:997	Paenibacillus tundrae A10bT (98.8 %)	971:1006	According to 16S RNA gene sequence analysis, the isolate is phylogenetically placed in the same cluster as Paenibacillus taichungensis BCRC 17757T (99.7 % nucleotide sequence identity) and Paenibacillus pabuli NBRC 13638T (99.1 %) and is closely related to Paenibacillus tundrae A10bT (98.8 %).
31483753	7	34	theme	tundrae	985:991	arg1	%					1005:1005	98.8 %	1000:1005	98.8 %	1000:1005	According to 16S RNA gene sequence analysis, the isolate is phylogenetically placed in the same cluster as Paenibacillus taichungensis BCRC 17757T (99.7 % nucleotide sequence identity) and Paenibacillus pabuli NBRC 13638T (99.1 %) and is closely related to Paenibacillus tundrae A10bT (98.8 %).
31483753	9	35	theme	average	1186:1192	arg1	gANI					1224:1227	whole genome average nucleotide identity analysis (gANI)	1173:1228	whole genome average nucleotide identity analysis (gANI) with related type strains	1173:1254	Furthermore, the results of whole genome average nucleotide identity analysis (gANI) with related type strains was lower than 91.10 % and the digital DNA-DNA hybridization value was lower than 44.30 %, which are below the threshold values for separating two species.
31483753	10	36	theme	genome	1470:1475	arg1	sequencing					1477:1486	genome sequencing	1470:1486	genome sequencing	1470:1486	The DNA G+C content was estimated as 46.09 mol%, based on genome sequencing.
31483753	9	37	theme	identity	1205:1212	arg1	gANI					1224:1227	whole genome average nucleotide identity analysis (gANI)	1173:1228	whole genome average nucleotide identity analysis (gANI) with related type strains	1173:1254	Furthermore, the results of whole genome average nucleotide identity analysis (gANI) with related type strains was lower than 91.10 % and the digital DNA-DNA hybridization value was lower than 44.30 %, which are below the threshold values for separating two species.
31483753	0	38	dep	sp	27:28	arg1	xylanivorans					14:25	Paenibacillus xylanivorans	0:25	Paenibacillus xylanivorans	0:25	Paenibacillus xylanivorans sp.
31483753	4	39	theme	optimal	320:326	arg1	30 °C					350:354	30 °C	350:354	30 °C (range, 28-37 °C)	350:372	Its optimal growth conditions are 30 °C (range, 28-37 °C), pH 7 (range, pH 5-10) and it tolerates up to 7 % of NaCl (range, 2-7 %).
31483753	4	39	theme	optimal	320:326	arg1	conditions					335:344	Its optimal growth conditions	316:344	Its optimal growth conditions	316:344	Its optimal growth conditions are 30 °C (range, 28-37 °C), pH 7 (range, pH 5-10) and it tolerates up to 7 % of NaCl (range, 2-7 %).
31483753	2	40	theme	soil	172:175	arg1	consortium					177:186	a forest soil consortium	163:186	a forest soil consortium in southern Argentina	163:208	A xylanolytic bacterial strain, named A59T, was isolated from a forest soil consortium in southern Argentina.
31483753	9	41	with	gANI	1224:1227	arg1	strains					1248:1254	related type strains	1235:1254	related type strains	1235:1254	Furthermore, the results of whole genome average nucleotide identity analysis (gANI) with related type strains was lower than 91.10 % and the digital DNA-DNA hybridization value was lower than 44.30 %, which are below the threshold values for separating two species.
31483753	1	42	theme	xylan-degrading	39:53	arg1	bacterium					55:63	a xylan-degrading bacterium	37:63	a xylan-degrading bacterium	37:63	nov., a xylan-degrading bacterium isolated from decaying forest soil.
31483753	1	42	theme	xylan-degrading	39:53	arg1	nov.					31:34	nov.	31:34	nov.	31:34	nov., a xylan-degrading bacterium isolated from decaying forest soil.
31483753	13	43	theme	type	1641:1644	arg1	A59T					1656:1659	A59T	1656:1659	A59T (=DSM 107920T=NCIMB 15123T)	1656:1687	The type strain is A59T (=DSM 107920T=NCIMB 15123T).
31483753	13	43	theme	type	1641:1644	arg1	strain					1646:1651	The type strain	1637:1651	The type strain	1637:1651	The type strain is A59T (=DSM 107920T=NCIMB 15123T).
31483753	2	44	theme	xylanolytic	103:113	arg1	strain					125:130	A xylanolytic bacterial strain	101:130	A xylanolytic bacterial strain	101:130	A xylanolytic bacterial strain, named A59T, was isolated from a forest soil consortium in southern Argentina.
31483753	7	45	theme	gene	735:738	arg1	analysis					749:756	16S RNA gene sequence analysis	727:756	16S RNA gene sequence analysis	727:756	According to 16S RNA gene sequence analysis, the isolate is phylogenetically placed in the same cluster as Paenibacillus taichungensis BCRC 17757T (99.7 % nucleotide sequence identity) and Paenibacillus pabuli NBRC 13638T (99.1 %) and is closely related to Paenibacillus tundrae A10bT (98.8 %).
31483753	9	46	theme	related	1235:1241	arg1	strains					1248:1254	related type strains	1235:1254	related type strains	1235:1254	Furthermore, the results of whole genome average nucleotide identity analysis (gANI) with related type strains was lower than 91.10 % and the digital DNA-DNA hybridization value was lower than 44.30 %, which are below the threshold values for separating two species.
31483753	7	47	theme	16S	727:729	arg1	analysis					749:756	16S RNA gene sequence analysis	727:756	16S RNA gene sequence analysis	727:756	According to 16S RNA gene sequence analysis, the isolate is phylogenetically placed in the same cluster as Paenibacillus taichungensis BCRC 17757T (99.7 % nucleotide sequence identity) and Paenibacillus pabuli NBRC 13638T (99.1 %) and is closely related to Paenibacillus tundrae A10bT (98.8 %).
31483753	8	48	theme	gyrB	1065:1068	arg1	gene					1070:1073	the housekeeping gyrB gene	1048:1073	the housekeeping gyrB gene	1048:1073	However, phylogenetic studies based on the housekeeping gyrB gene placed A59T in a separate branch from all other related type strains.
31483753	6	49	theme	moderate	678:685	arg1	C16 					697:700	C16 	697:700	C16 	697:700	It contains menaquinone MK-7 as the predominant isoprenoid quinone and the major fatty acid is anteiso-C15 : 0 (35.1 %), with a moderate amount of C16 : 0 (6.9 %).
31483753	6	49	theme	moderate	678:685	arg1	amount					687:692	a moderate amount	676:692	a moderate amount of C16 	676:700	It contains menaquinone MK-7 as the predominant isoprenoid quinone and the major fatty acid is anteiso-C15 : 0 (35.1 %), with a moderate amount of C16 : 0 (6.9 %).
31483753	11	50	theme	Paenibacillus	1563:1575	arg1	genus					1557:1561	the genus Paenibacillus	1553:1575	the genus Paenibacillus	1553:1575	On the basis of these results, A59T represents a new species of the genus Paenibacillus, and we propose the name Paenibacillusxylanivorans sp.
31483753	8	51	theme	phylogenetic	1018:1029	arg1	studies					1031:1037	phylogenetic studies	1018:1037	phylogenetic studies based on the housekeeping gyrB gene	1018:1073	However, phylogenetic studies based on the housekeeping gyrB gene placed A59T in a separate branch from all other related type strains.
31483753	3	52	theme	endospore-forming	272:288	arg1	A59T					218:221	Strain A59T	211:221	Strain A59T	211:221	Strain A59T is a Gram-stain-positive, facultative anaerobic, endospore-forming and rod-shaped bacterium.
31483753	3	52	theme	endospore-forming	272:288	arg1	bacterium					305:313	a Gram-stain-positive, facultative anaerobic, endospore-forming and rod-shaped bacterium	226:313	a Gram-stain-positive, facultative anaerobic, endospore-forming and rod-shaped bacterium	226:313	Strain A59T is a Gram-stain-positive, facultative anaerobic, endospore-forming and rod-shaped bacterium.
31483753	10	53	theme	G+C	1420:1422	arg1	%					1458:1458	46.09 mol%	1449:1458	46.09 mol%	1449:1458	The DNA G+C content was estimated as 46.09 mol%, based on genome sequencing.
31483753	10	53	theme	G+C	1420:1422	arg1	content					1424:1430	The DNA G+C content	1412:1430	The DNA G+C content	1412:1430	The DNA G+C content was estimated as 46.09 mol%, based on genome sequencing.
31483753	7	54	theme	pabuli	917:922	arg1	isolate					763:769	the isolate	759:769	the isolate	759:769	According to 16S RNA gene sequence analysis, the isolate is phylogenetically placed in the same cluster as Paenibacillus taichungensis BCRC 17757T (99.7 % nucleotide sequence identity) and Paenibacillus pabuli NBRC 13638T (99.1 %) and is closely related to Paenibacillus tundrae A10bT (98.8 %).
31483753	7	54	theme	pabuli	917:922	arg1	13638T					929:934	Paenibacillus pabuli NBRC 13638T	903:934	Paenibacillus pabuli NBRC 13638T (99.1 %)	903:943	According to 16S RNA gene sequence analysis, the isolate is phylogenetically placed in the same cluster as Paenibacillus taichungensis BCRC 17757T (99.7 % nucleotide sequence identity) and Paenibacillus pabuli NBRC 13638T (99.1 %) and is closely related to Paenibacillus tundrae A10bT (98.8 %).
31483753	7	54	theme	pabuli	917:922	arg1	%					942:942	99.1 %	937:942	99.1 %	937:942	According to 16S RNA gene sequence analysis, the isolate is phylogenetically placed in the same cluster as Paenibacillus taichungensis BCRC 17757T (99.7 % nucleotide sequence identity) and Paenibacillus pabuli NBRC 13638T (99.1 %) and is closely related to Paenibacillus tundrae A10bT (98.8 %).
31483753	11	55	dep	results	1511:1517	arg1	the					1492:1494	the	1492:1494	the	1492:1494	On the basis of these results, A59T represents a new species of the genus Paenibacillus, and we propose the name Paenibacillusxylanivorans sp.
31483753	11	55	dep	results	1511:1517	arg1	basis					1496:1500	basis	1496:1500	basis	1496:1500	On the basis of these results, A59T represents a new species of the genus Paenibacillus, and we propose the name Paenibacillusxylanivorans sp.
31483753	3	56	theme	anaerobic	261:269	arg1	A59T					218:221	Strain A59T	211:221	Strain A59T	211:221	Strain A59T is a Gram-stain-positive, facultative anaerobic, endospore-forming and rod-shaped bacterium.
31483753	3	56	theme	anaerobic	261:269	arg1	bacterium					305:313	a Gram-stain-positive, facultative anaerobic, endospore-forming and rod-shaped bacterium	226:313	a Gram-stain-positive, facultative anaerobic, endospore-forming and rod-shaped bacterium	226:313	Strain A59T is a Gram-stain-positive, facultative anaerobic, endospore-forming and rod-shaped bacterium.
31483753	8	57	theme	separate	1092:1099	arg1	branch					1101:1106	a separate branch	1090:1106	a separate branch from all other related type strains	1090:1142	However, phylogenetic studies based on the housekeeping gyrB gene placed A59T in a separate branch from all other related type strains.
31483753	3	58	dep	Gram-stain-positive	228:246	arg1	facultative					249:259	facultative	249:259	facultative	249:259	Strain A59T is a Gram-stain-positive, facultative anaerobic, endospore-forming and rod-shaped bacterium.
31483753	7	59	theme	sequence	880:887	arg1	17757T					854:859	Paenibacillus taichungensis BCRC 17757T	821:859	Paenibacillus taichungensis BCRC 17757T (99.7 % nucleotide sequence identity)	821:897	According to 16S RNA gene sequence analysis, the isolate is phylogenetically placed in the same cluster as Paenibacillus taichungensis BCRC 17757T (99.7 % nucleotide sequence identity) and Paenibacillus pabuli NBRC 13638T (99.1 %) and is closely related to Paenibacillus tundrae A10bT (98.8 %).
31483753	7	59	theme	sequence	880:887	arg1	identity					889:896	99.7 % nucleotide sequence identity	862:896	99.7 % nucleotide sequence identity	862:896	According to 16S RNA gene sequence analysis, the isolate is phylogenetically placed in the same cluster as Paenibacillus taichungensis BCRC 17757T (99.7 % nucleotide sequence identity) and Paenibacillus pabuli NBRC 13638T (99.1 %) and is closely related to Paenibacillus tundrae A10bT (98.8 %).
31483753	9	60	theme	digital	1287:1293	arg1	hybridization					1303:1315	digital DNA-DNA hybridization	1287:1315	the digital DNA-DNA hybridization value	1283:1321	Furthermore, the results of whole genome average nucleotide identity analysis (gANI) with related type strains was lower than 91.10 % and the digital DNA-DNA hybridization value was lower than 44.30 %, which are below the threshold values for separating two species.
31483753	6	61	theme	predominant	586:596	arg1	quinone					609:615	the predominant isoprenoid quinone	582:615	the predominant isoprenoid quinone	582:615	It contains menaquinone MK-7 as the predominant isoprenoid quinone and the major fatty acid is anteiso-C15 : 0 (35.1 %), with a moderate amount of C16 : 0 (6.9 %).
31483753	6	61	theme	predominant	586:596	arg1	MK-7					574:577	menaquinone MK-7	562:577	menaquinone MK-7	562:577	It contains menaquinone MK-7 as the predominant isoprenoid quinone and the major fatty acid is anteiso-C15 : 0 (35.1 %), with a moderate amount of C16 : 0 (6.9 %).
31483753	7	62	theme	%	867:867	arg1	17757T					854:859	Paenibacillus taichungensis BCRC 17757T	821:859	Paenibacillus taichungensis BCRC 17757T (99.7 % nucleotide sequence identity)	821:897	According to 16S RNA gene sequence analysis, the isolate is phylogenetically placed in the same cluster as Paenibacillus taichungensis BCRC 17757T (99.7 % nucleotide sequence identity) and Paenibacillus pabuli NBRC 13638T (99.1 %) and is closely related to Paenibacillus tundrae A10bT (98.8 %).
31483753	7	62	theme	%	867:867	arg1	identity					889:896	99.7 % nucleotide sequence identity	862:896	99.7 % nucleotide sequence identity	862:896	According to 16S RNA gene sequence analysis, the isolate is phylogenetically placed in the same cluster as Paenibacillus taichungensis BCRC 17757T (99.7 % nucleotide sequence identity) and Paenibacillus pabuli NBRC 13638T (99.1 %) and is closely related to Paenibacillus tundrae A10bT (98.8 %).
31483753	9	63	theme	hybridization	1303:1315	arg1	%					1344:1344	lower than 44.30 %	1327:1344	lower than 44.30 %	1327:1344	Furthermore, the results of whole genome average nucleotide identity analysis (gANI) with related type strains was lower than 91.10 % and the digital DNA-DNA hybridization value was lower than 44.30 %, which are below the threshold values for separating two species.
31483753	9	63	theme	hybridization	1303:1315	arg1	value					1317:1321	the digital DNA-DNA hybridization value	1283:1321	the digital DNA-DNA hybridization value	1283:1321	Furthermore, the results of whole genome average nucleotide identity analysis (gANI) with related type strains was lower than 91.10 % and the digital DNA-DNA hybridization value was lower than 44.30 %, which are below the threshold values for separating two species.
31483753	3	64	theme	rod-shaped	294:303	arg1	A59T					218:221	Strain A59T	211:221	Strain A59T	211:221	Strain A59T is a Gram-stain-positive, facultative anaerobic, endospore-forming and rod-shaped bacterium.
31483753	3	64	theme	rod-shaped	294:303	arg1	bacterium					305:313	a Gram-stain-positive, facultative anaerobic, endospore-forming and rod-shaped bacterium	226:313	a Gram-stain-positive, facultative anaerobic, endospore-forming and rod-shaped bacterium	226:313	Strain A59T is a Gram-stain-positive, facultative anaerobic, endospore-forming and rod-shaped bacterium.
31483753	8	65	theme	other	1117:1121	arg1	strains					1136:1142	all other related type strains	1113:1142	all other related type strains	1113:1142	However, phylogenetic studies based on the housekeeping gyrB gene placed A59T in a separate branch from all other related type strains.
31483753	13	66	theme	107920T=NCIMB	1667:1679	arg1	A59T					1656:1659	A59T	1656:1659	A59T (=DSM 107920T=NCIMB 15123T)	1656:1687	The type strain is A59T (=DSM 107920T=NCIMB 15123T).
31483753	13	66	theme	107920T=NCIMB	1667:1679	arg1	15123T					1681:1686	=DSM 107920T=NCIMB 15123T	1662:1686	=DSM 107920T=NCIMB 15123T	1662:1686	The type strain is A59T (=DSM 107920T=NCIMB 15123T).
31483753	8	67	theme	type	1131:1134	arg1	strains					1136:1142	all other related type strains	1113:1142	all other related type strains	1113:1142	However, phylogenetic studies based on the housekeeping gyrB gene placed A59T in a separate branch from all other related type strains.
31483753	6	68	theme	menaquinone	562:572	arg1	quinone					609:615	the predominant isoprenoid quinone	582:615	the predominant isoprenoid quinone	582:615	It contains menaquinone MK-7 as the predominant isoprenoid quinone and the major fatty acid is anteiso-C15 : 0 (35.1 %), with a moderate amount of C16 : 0 (6.9 %).
31483753	6	68	theme	menaquinone	562:572	arg1	MK-7					574:577	menaquinone MK-7	562:577	menaquinone MK-7	562:577	It contains menaquinone MK-7 as the predominant isoprenoid quinone and the major fatty acid is anteiso-C15 : 0 (35.1 %), with a moderate amount of C16 : 0 (6.9 %).
31483753	7	69	theme	BCRC	849:852	arg1	17757T					854:859	Paenibacillus taichungensis BCRC 17757T	821:859	Paenibacillus taichungensis BCRC 17757T (99.7 % nucleotide sequence identity)	821:897	According to 16S RNA gene sequence analysis, the isolate is phylogenetically placed in the same cluster as Paenibacillus taichungensis BCRC 17757T (99.7 % nucleotide sequence identity) and Paenibacillus pabuli NBRC 13638T (99.1 %) and is closely related to Paenibacillus tundrae A10bT (98.8 %).
31483753	7	69	theme	BCRC	849:852	arg1	identity					889:896	99.7 % nucleotide sequence identity	862:896	99.7 % nucleotide sequence identity	862:896	According to 16S RNA gene sequence analysis, the isolate is phylogenetically placed in the same cluster as Paenibacillus taichungensis BCRC 17757T (99.7 % nucleotide sequence identity) and Paenibacillus pabuli NBRC 13638T (99.1 %) and is closely related to Paenibacillus tundrae A10bT (98.8 %).
31483753	7	69	theme	BCRC	849:852	arg1	isolate					763:769	the isolate	759:769	the isolate	759:769	According to 16S RNA gene sequence analysis, the isolate is phylogenetically placed in the same cluster as Paenibacillus taichungensis BCRC 17757T (99.7 % nucleotide sequence identity) and Paenibacillus pabuli NBRC 13638T (99.1 %) and is closely related to Paenibacillus tundrae A10bT (98.8 %).
31483753	7	70	theme	Paenibacillus	821:833	arg1	17757T					854:859	Paenibacillus taichungensis BCRC 17757T	821:859	Paenibacillus taichungensis BCRC 17757T (99.7 % nucleotide sequence identity)	821:897	According to 16S RNA gene sequence analysis, the isolate is phylogenetically placed in the same cluster as Paenibacillus taichungensis BCRC 17757T (99.7 % nucleotide sequence identity) and Paenibacillus pabuli NBRC 13638T (99.1 %) and is closely related to Paenibacillus tundrae A10bT (98.8 %).
31483753	7	70	theme	Paenibacillus	821:833	arg1	identity					889:896	99.7 % nucleotide sequence identity	862:896	99.7 % nucleotide sequence identity	862:896	According to 16S RNA gene sequence analysis, the isolate is phylogenetically placed in the same cluster as Paenibacillus taichungensis BCRC 17757T (99.7 % nucleotide sequence identity) and Paenibacillus pabuli NBRC 13638T (99.1 %) and is closely related to Paenibacillus tundrae A10bT (98.8 %).
31483753	7	70	theme	Paenibacillus	821:833	arg1	isolate					763:769	the isolate	759:769	the isolate	759:769	According to 16S RNA gene sequence analysis, the isolate is phylogenetically placed in the same cluster as Paenibacillus taichungensis BCRC 17757T (99.7 % nucleotide sequence identity) and Paenibacillus pabuli NBRC 13638T (99.1 %) and is closely related to Paenibacillus tundrae A10bT (98.8 %).
31483753	6	71	theme	fatty	631:635	arg1	anteiso-C15 					645:656	anteiso-C15 	645:656	anteiso-C15 	645:656	It contains menaquinone MK-7 as the predominant isoprenoid quinone and the major fatty acid is anteiso-C15 : 0 (35.1 %), with a moderate amount of C16 : 0 (6.9 %).
31483753	6	71	theme	fatty	631:635	arg1	acid					637:640	the major fatty acid	621:640	the major fatty acid	621:640	It contains menaquinone MK-7 as the predominant isoprenoid quinone and the major fatty acid is anteiso-C15 : 0 (35.1 %), with a moderate amount of C16 : 0 (6.9 %).
31483753	1	72	theme	forest	88:93	arg1	soil					95:98	forest soil	88:98	forest soil	88:98	nov., a xylan-degrading bacterium isolated from decaying forest soil.
31483753	4	73	dep	%	422:422	arg1	range					433:437	range	433:437	range	433:437	Its optimal growth conditions are 30 °C (range, 28-37 °C), pH 7 (range, pH 5-10) and it tolerates up to 7 % of NaCl (range, 2-7 %).
31483753	4	73	dep	%	422:422	arg1	%					444:444	2-7 %	440:444	2-7 %	440:444	Its optimal growth conditions are 30 °C (range, 28-37 °C), pH 7 (range, pH 5-10) and it tolerates up to 7 % of NaCl (range, 2-7 %).
31483753	9	74	theme	threshold	1367:1375	arg1	values					1377:1382	the threshold values	1363:1382	the threshold values for separating two species	1363:1409	Furthermore, the results of whole genome average nucleotide identity analysis (gANI) with related type strains was lower than 91.10 % and the digital DNA-DNA hybridization value was lower than 44.30 %, which are below the threshold values for separating two species.
31483753	2	75	theme	forest	165:170	arg1	soil					172:175	a forest soil	163:175	a forest soil consortium in southern Argentina	163:208	A xylanolytic bacterial strain, named A59T, was isolated from a forest soil consortium in southern Argentina.
31483753	4	76	dep	30 °C	350:354	arg1	28-37 °C					364:371	28-37 °C	364:371	28-37 °C	364:371	Its optimal growth conditions are 30 °C (range, 28-37 °C), pH 7 (range, pH 5-10) and it tolerates up to 7 % of NaCl (range, 2-7 %).
31483753	4	76	dep	30 °C	350:354	arg1	range					357:361	range	357:361	range	357:361	Its optimal growth conditions are 30 °C (range, 28-37 °C), pH 7 (range, pH 5-10) and it tolerates up to 7 % of NaCl (range, 2-7 %).
31483753	2	77	attach	isolated	149:156	arg1	consortium					177:186	a forest soil consortium	163:186	a forest soil consortium in southern Argentina	163:208	A xylanolytic bacterial strain, named A59T, was isolated from a forest soil consortium in southern Argentina.
31483753	2	77	attach	isolated	149:156	arg2	strain					125:130	A xylanolytic bacterial strain	101:130	A xylanolytic bacterial strain	101:130	A xylanolytic bacterial strain, named A59T, was isolated from a forest soil consortium in southern Argentina.
31483753	10	78	theme	46.09 mol	1449:1457	arg1	%					1458:1458	46.09 mol%	1449:1458	46.09 mol%	1449:1458	The DNA G+C content was estimated as 46.09 mol%, based on genome sequencing.
31483753	10	78	theme	46.09 mol	1449:1457	arg1	content					1424:1430	The DNA G+C content	1412:1430	The DNA G+C content	1412:1430	The DNA G+C content was estimated as 46.09 mol%, based on genome sequencing.
31483753	7	79	theme	Paenibacillus	971:983	arg1	A10bT					993:997	Paenibacillus tundrae A10bT	971:997	Paenibacillus tundrae A10bT (98.8 %)	971:1006	According to 16S RNA gene sequence analysis, the isolate is phylogenetically placed in the same cluster as Paenibacillus taichungensis BCRC 17757T (99.7 % nucleotide sequence identity) and Paenibacillus pabuli NBRC 13638T (99.1 %) and is closely related to Paenibacillus tundrae A10bT (98.8 %).
31483753	7	79	theme	Paenibacillus	971:983	arg1	%					1005:1005	98.8 %	1000:1005	98.8 %	1000:1005	According to 16S RNA gene sequence analysis, the isolate is phylogenetically placed in the same cluster as Paenibacillus taichungensis BCRC 17757T (99.7 % nucleotide sequence identity) and Paenibacillus pabuli NBRC 13638T (99.1 %) and is closely related to Paenibacillus tundrae A10bT (98.8 %).
31483753	9	80	theme	genome	1179:1184	arg1	gANI					1224:1227	whole genome average nucleotide identity analysis (gANI)	1173:1228	whole genome average nucleotide identity analysis (gANI) with related type strains	1173:1254	Furthermore, the results of whole genome average nucleotide identity analysis (gANI) with related type strains was lower than 91.10 % and the digital DNA-DNA hybridization value was lower than 44.30 %, which are below the threshold values for separating two species.
31483753	2	81	theme	southern	191:198	arg1	Argentina					200:208	southern Argentina	191:208	southern Argentina	191:208	A xylanolytic bacterial strain, named A59T, was isolated from a forest soil consortium in southern Argentina.
31483753	9	82	theme	nucleotide	1194:1203	arg1	gANI					1224:1227	whole genome average nucleotide identity analysis (gANI)	1173:1228	whole genome average nucleotide identity analysis (gANI) with related type strains	1173:1254	Furthermore, the results of whole genome average nucleotide identity analysis (gANI) with related type strains was lower than 91.10 % and the digital DNA-DNA hybridization value was lower than 44.30 %, which are below the threshold values for separating two species.
31483753	6	83	dep	anteiso-C15 	645:656	arg1	 0					658:659	 0	658:659	 0	658:659	It contains menaquinone MK-7 as the predominant isoprenoid quinone and the major fatty acid is anteiso-C15 : 0 (35.1 %), with a moderate amount of C16 : 0 (6.9 %).
31483753	6	83	dep	anteiso-C15 	645:656	arg1	%					667:667	35.1 %	662:667	35.1 %	662:667	It contains menaquinone MK-7 as the predominant isoprenoid quinone and the major fatty acid is anteiso-C15 : 0 (35.1 %), with a moderate amount of C16 : 0 (6.9 %).
31483753	6	83	dep	anteiso-C15 	645:656	arg1	%					710:710	6.9 %	706:710	6.9 %	706:710	It contains menaquinone MK-7 as the predominant isoprenoid quinone and the major fatty acid is anteiso-C15 : 0 (35.1 %), with a moderate amount of C16 : 0 (6.9 %).
31483753	6	83	dep	anteiso-C15 	645:656	arg1	 0					702:703	 0	702:703	 0	702:703	It contains menaquinone MK-7 as the predominant isoprenoid quinone and the major fatty acid is anteiso-C15 : 0 (35.1 %), with a moderate amount of C16 : 0 (6.9 %).
31483753	5	84	theme	Chemotaxonomic	448:461	arg1	analysis					463:470	Chemotaxonomic analysis	448:470	Chemotaxonomic analysis	448:470	Chemotaxonomic analysis revealed that strain A59Tpossesses meso-diaminopimelic acid in the cell wall.
31483753	11	85	theme	name	1597:1600	arg1	sp					1628:1629	the name Paenibacillusxylanivorans sp	1593:1629	the name Paenibacillusxylanivorans sp	1593:1629	On the basis of these results, A59T represents a new species of the genus Paenibacillus, and we propose the name Paenibacillusxylanivorans sp.
31483753	9	86	theme	analysis	1214:1221	arg1	gANI					1224:1227	whole genome average nucleotide identity analysis (gANI)	1173:1228	whole genome average nucleotide identity analysis (gANI) with related type strains	1173:1254	Furthermore, the results of whole genome average nucleotide identity analysis (gANI) with related type strains was lower than 91.10 % and the digital DNA-DNA hybridization value was lower than 44.30 %, which are below the threshold values for separating two species.
31483753	7	87	theme	sequence	740:747	arg1	analysis					749:756	16S RNA gene sequence analysis	727:756	16S RNA gene sequence analysis	727:756	According to 16S RNA gene sequence analysis, the isolate is phylogenetically placed in the same cluster as Paenibacillus taichungensis BCRC 17757T (99.7 % nucleotide sequence identity) and Paenibacillus pabuli NBRC 13638T (99.1 %) and is closely related to Paenibacillus tundrae A10bT (98.8 %).
31483753	9	88	theme	gANI	1224:1227	arg1	results					1162:1168	the results	1158:1168	the results of whole genome average nucleotide identity analysis (gANI) with related type strains	1158:1254	Furthermore, the results of whole genome average nucleotide identity analysis (gANI) with related type strains was lower than 91.10 % and the digital DNA-DNA hybridization value was lower than 44.30 %, which are below the threshold values for separating two species.
31483753	9	88	theme	gANI	1224:1227	arg1	%					1277:1277	lower than 91.10 %	1260:1277	lower than 91.10 %	1260:1277	Furthermore, the results of whole genome average nucleotide identity analysis (gANI) with related type strains was lower than 91.10 % and the digital DNA-DNA hybridization value was lower than 44.30 %, which are below the threshold values for separating two species.
30516875	7	0	theme	CONCLUSION	1192:1201	arg1	PTSO					1203:1206	CONCLUSION PTSO	1192:1206	CONCLUSION PTSO	1192:1206	CONCLUSION PTSO exerts intestinal anti-inflammatory activity in experimental colitis in mice.
30516875	7	1	theme	experimental	1256:1267	arg1	colitis					1269:1275	experimental colitis	1256:1275	experimental colitis in mice	1256:1283	CONCLUSION PTSO exerts intestinal anti-inflammatory activity in experimental colitis in mice.
30516875	4	2	theme	PTSO	693:696	arg1	effects					682:688	The immunomodulatory effects	661:688	The immunomodulatory effects of PTSO (0.1-25 µm)	661:708	The immunomodulatory effects of PTSO (0.1-25 µm) are also shown in vitro in Caco-2 and THP-1 cells, reducing the production of pro-inflammatory mediators and downregulating mitogen-activated protein kinases (MAPKs) signaling pathways.
30516875	9	3	from	development	1632:1642	arg1	IBD					1653:1655	human IBD	1647:1655	human IBD	1647:1655	Furthermore, PTSO is able to restore the intestinal epithelial barrier function and to ameliorate the intestinal microbiota homeostasis, thus supporting its future development in human IBD.
30516875	9	4	theme	human	1647:1651	arg1	IBD					1653:1655	human IBD	1647:1655	human IBD	1647:1655	Furthermore, PTSO is able to restore the intestinal epithelial barrier function and to ameliorate the intestinal microbiota homeostasis, thus supporting its future development in human IBD.
30516875	0	5	theme	Anti-Inflammatory	93:109	arg1	Effect					111:116	its Intestinal Anti-Inflammatory Effect	78:116	its Intestinal Anti-Inflammatory Effect in Experimental Colitis	78:140	The Immunomodulatory Properties of Propyl-Propane Thiosulfonate Contribute to its Intestinal Anti-Inflammatory Effect in Experimental Colitis.
30516875	4	6	theme	kinases	860:866	arg1	pathways					886:893	mitogen-activated protein kinases (MAPKs) signaling pathways	834:893	mitogen-activated protein kinases (MAPKs) signaling pathways	834:893	The immunomodulatory effects of PTSO (0.1-25 µm) are also shown in vitro in Caco-2 and THP-1 cells, reducing the production of pro-inflammatory mediators and downregulating mitogen-activated protein kinases (MAPKs) signaling pathways.
30516875	9	7	theme	intestinal	1509:1518	arg1	function					1539:1546	the intestinal epithelial barrier function	1505:1546	the intestinal epithelial barrier function	1505:1546	Furthermore, PTSO is able to restore the intestinal epithelial barrier function and to ameliorate the intestinal microbiota homeostasis, thus supporting its future development in human IBD.
30516875	3	8	theme	2,4-dinitrobenzene	524:541	arg1	doses					570:574	PTSO doses	565:574	PTSO doses: 0.01-10 mg kg-1	565:591	METHODS AND RESULTS The anti-inflammatory effects of PTSO are studied in two mice models of colitis: 2,4-dinitrobenzene sulfonic acid (DNBS) (PTSO doses: 0.01-10 mg kg-1 ) and dextran sodium sulfate (DSS) (PTSO doses: 0.01-0.1 mg kg-1 ).
30516875	3	8	theme	2,4-dinitrobenzene	524:541	arg1	DNBS					558:561	DNBS	558:561	DNBS	558:561	METHODS AND RESULTS The anti-inflammatory effects of PTSO are studied in two mice models of colitis: 2,4-dinitrobenzene sulfonic acid (DNBS) (PTSO doses: 0.01-10 mg kg-1 ) and dextran sodium sulfate (DSS) (PTSO doses: 0.01-0.1 mg kg-1 ).
30516875	3	8	theme	2,4-dinitrobenzene	524:541	arg1	METHODS					423:429	METHODS	423:429	METHODS	423:429	METHODS AND RESULTS The anti-inflammatory effects of PTSO are studied in two mice models of colitis: 2,4-dinitrobenzene sulfonic acid (DNBS) (PTSO doses: 0.01-10 mg kg-1 ) and dextran sodium sulfate (DSS) (PTSO doses: 0.01-0.1 mg kg-1 ).
30516875	3	8	theme	2,4-dinitrobenzene	524:541	arg1	acid					552:555	2,4-dinitrobenzene sulfonic acid	524:555	2,4-dinitrobenzene sulfonic acid (DNBS) (PTSO doses: 0.01-10 mg kg-1 )	524:593	METHODS AND RESULTS The anti-inflammatory effects of PTSO are studied in two mice models of colitis: 2,4-dinitrobenzene sulfonic acid (DNBS) (PTSO doses: 0.01-10 mg kg-1 ) and dextran sodium sulfate (DSS) (PTSO doses: 0.01-0.1 mg kg-1 ).
30516875	0	9	from	Effect	111:116	arg1	Colitis					134:140	Experimental Colitis	121:140	Experimental Colitis	121:140	The Immunomodulatory Properties of Propyl-Propane Thiosulfonate Contribute to its Intestinal Anti-Inflammatory Effect in Experimental Colitis.
30516875	0	10	theme	Experimental	121:132	arg1	Colitis					134:140	Experimental Colitis	121:140	Experimental Colitis	121:140	The Immunomodulatory Properties of Propyl-Propane Thiosulfonate Contribute to its Intestinal Anti-Inflammatory Effect in Experimental Colitis.
30516875	5	11	theme	mouse	956:960	arg1	colitis					962:968	mouse colitis	956:968	mouse colitis	956:968	This compound displays beneficial effects in both models of mouse colitis by reducing the expression of different pro-inflammatory mediators and improving the intestinal epithelial barrier integrity.
30516875	2	12	from	candidate	363:371	arg1	consequence					326:336	consequence	326:336	consequence	326:336	In consequence, PTSO can be a potential candidate for the treatment of inflammatory bowel diseases.
30516875	5	13	theme	colitis	962:968	arg1	models					946:951	both models	941:951	both models of mouse colitis	941:968	This compound displays beneficial effects in both models of mouse colitis by reducing the expression of different pro-inflammatory mediators and improving the intestinal epithelial barrier integrity.
30516875	9	14	theme	epithelial	1520:1529	arg1	function					1539:1546	the intestinal epithelial barrier function	1505:1546	the intestinal epithelial barrier function	1505:1546	Furthermore, PTSO is able to restore the intestinal epithelial barrier function and to ameliorate the intestinal microbiota homeostasis, thus supporting its future development in human IBD.
30516875	6	15	theme	gut	1135:1137	arg1	composition					1150:1160	the altered gut microbiota composition	1123:1160	the altered gut microbiota composition observed in DSS colitic mice	1123:1189	Moreover, PTSO ameliorates the altered gut microbiota composition observed in DSS colitic mice.
30516875	7	16	theme	intestinal	1215:1224	arg1	activity					1244:1251	intestinal anti-inflammatory activity	1215:1251	intestinal anti-inflammatory activity	1215:1251	CONCLUSION PTSO exerts intestinal anti-inflammatory activity in experimental colitis in mice.
30516875	8	17	theme	immunomodulatory	1345:1360	arg1	properties					1362:1371	the immunomodulatory properties	1341:1371	the immunomodulatory properties of PTSO	1341:1379	This anti-inflammatory activity can be associated with the immunomodulatory properties of PTSO through the regulation of the activity of cells involved in the inflammatory response.
30516875	3	18	dep	METHODS	423:429	arg1	METHODS					423:429	METHODS	423:429	METHODS	423:429	METHODS AND RESULTS The anti-inflammatory effects of PTSO are studied in two mice models of colitis: 2,4-dinitrobenzene sulfonic acid (DNBS) (PTSO doses: 0.01-10 mg kg-1 ) and dextran sodium sulfate (DSS) (PTSO doses: 0.01-0.1 mg kg-1 ).
30516875	3	18	dep	METHODS	423:429	arg1	acid					552:555	2,4-dinitrobenzene sulfonic acid	524:555	2,4-dinitrobenzene sulfonic acid (DNBS) (PTSO doses: 0.01-10 mg kg-1 )	524:593	METHODS AND RESULTS The anti-inflammatory effects of PTSO are studied in two mice models of colitis: 2,4-dinitrobenzene sulfonic acid (DNBS) (PTSO doses: 0.01-10 mg kg-1 ) and dextran sodium sulfate (DSS) (PTSO doses: 0.01-0.1 mg kg-1 ).
30516875	3	18	dep	METHODS	423:429	arg1	doses					634:638	PTSO doses	629:638	PTSO doses: 0.01-0.1 mg kg-1	629:656	METHODS AND RESULTS The anti-inflammatory effects of PTSO are studied in two mice models of colitis: 2,4-dinitrobenzene sulfonic acid (DNBS) (PTSO doses: 0.01-10 mg kg-1 ) and dextran sodium sulfate (DSS) (PTSO doses: 0.01-0.1 mg kg-1 ).
30516875	3	18	dep	METHODS	423:429	arg1	doses					570:574	PTSO doses	565:574	PTSO doses: 0.01-10 mg kg-1	565:591	METHODS AND RESULTS The anti-inflammatory effects of PTSO are studied in two mice models of colitis: 2,4-dinitrobenzene sulfonic acid (DNBS) (PTSO doses: 0.01-10 mg kg-1 ) and dextran sodium sulfate (DSS) (PTSO doses: 0.01-0.1 mg kg-1 ).
30516875	3	18	dep	METHODS	423:429	arg1	sulfate					614:620	dextran sodium sulfate	599:620	dextran sodium sulfate (DSS) (PTSO doses: 0.01-0.1 mg kg-1 )	599:658	METHODS AND RESULTS The anti-inflammatory effects of PTSO are studied in two mice models of colitis: 2,4-dinitrobenzene sulfonic acid (DNBS) (PTSO doses: 0.01-10 mg kg-1 ) and dextran sodium sulfate (DSS) (PTSO doses: 0.01-0.1 mg kg-1 ).
30516875	3	18	dep	METHODS	423:429	arg1	DSS					623:625	DSS	623:625	DSS	623:625	METHODS AND RESULTS The anti-inflammatory effects of PTSO are studied in two mice models of colitis: 2,4-dinitrobenzene sulfonic acid (DNBS) (PTSO doses: 0.01-10 mg kg-1 ) and dextran sodium sulfate (DSS) (PTSO doses: 0.01-0.1 mg kg-1 ).
30516875	3	18	dep	METHODS	423:429	arg1	DNBS					558:561	DNBS	558:561	DNBS	558:561	METHODS AND RESULTS The anti-inflammatory effects of PTSO are studied in two mice models of colitis: 2,4-dinitrobenzene sulfonic acid (DNBS) (PTSO doses: 0.01-10 mg kg-1 ) and dextran sodium sulfate (DSS) (PTSO doses: 0.01-0.1 mg kg-1 ).
30516875	3	18	dep	METHODS	423:429	arg1	studied					485:491	studied	485:491	are studied in two mice models of colitis	481:521	METHODS AND RESULTS The anti-inflammatory effects of PTSO are studied in two mice models of colitis: 2,4-dinitrobenzene sulfonic acid (DNBS) (PTSO doses: 0.01-10 mg kg-1 ) and dextran sodium sulfate (DSS) (PTSO doses: 0.01-0.1 mg kg-1 ).
30516875	9	19	theme	barrier	1531:1537	arg1	function					1539:1546	the intestinal epithelial barrier function	1505:1546	the intestinal epithelial barrier function	1505:1546	Furthermore, PTSO is able to restore the intestinal epithelial barrier function and to ameliorate the intestinal microbiota homeostasis, thus supporting its future development in human IBD.
30516875	4	20	theme	THP-1	748:752	arg1	cells					754:758	THP-1 cells	748:758	THP-1 cells	748:758	The immunomodulatory effects of PTSO (0.1-25 µm) are also shown in vitro in Caco-2 and THP-1 cells, reducing the production of pro-inflammatory mediators and downregulating mitogen-activated protein kinases (MAPKs) signaling pathways.
30516875	3	21	theme	anti-inflammatory	447:463	arg1	effects					465:471	The anti-inflammatory effects	443:471	The anti-inflammatory effects of PTSO	443:479	METHODS AND RESULTS The anti-inflammatory effects of PTSO are studied in two mice models of colitis: 2,4-dinitrobenzene sulfonic acid (DNBS) (PTSO doses: 0.01-10 mg kg-1 ) and dextran sodium sulfate (DSS) (PTSO doses: 0.01-0.1 mg kg-1 ).
30516875	7	22	theme	anti-inflammatory	1226:1242	arg1	activity					1244:1251	intestinal anti-inflammatory activity	1215:1251	intestinal anti-inflammatory activity	1215:1251	CONCLUSION PTSO exerts intestinal anti-inflammatory activity in experimental colitis in mice.
30516875	6	23	theme	microbiota	1139:1148	arg1	composition					1150:1160	the altered gut microbiota composition	1123:1160	the altered gut microbiota composition observed in DSS colitic mice	1123:1189	Moreover, PTSO ameliorates the altered gut microbiota composition observed in DSS colitic mice.
30516875	8	24	theme	activity	1411:1418	arg1	regulation					1393:1402	the regulation	1389:1402	the regulation of the activity of cells involved in the inflammatory response	1389:1465	This anti-inflammatory activity can be associated with the immunomodulatory properties of PTSO through the regulation of the activity of cells involved in the inflammatory response.
30516875	2	25	theme	diseases	413:420	arg1	treatment					381:389	the treatment	377:389	the treatment of inflammatory bowel diseases	377:420	In consequence, PTSO can be a potential candidate for the treatment of inflammatory bowel diseases.
30516875	0	26	theme	Immunomodulatory	4:19	arg1	Properties					21:30	The Immunomodulatory Properties	0:30	The Immunomodulatory Properties of Propyl-Propane Thiosulfonate	0:62	The Immunomodulatory Properties of Propyl-Propane Thiosulfonate Contribute to its Intestinal Anti-Inflammatory Effect in Experimental Colitis.
30516875	4	27	theme	protein	852:858	arg1	MAPKs					869:873	MAPKs	869:873	MAPKs	869:873	The immunomodulatory effects of PTSO (0.1-25 µm) are also shown in vitro in Caco-2 and THP-1 cells, reducing the production of pro-inflammatory mediators and downregulating mitogen-activated protein kinases (MAPKs) signaling pathways.
30516875	4	27	theme	protein	852:858	arg1	kinases					860:866	mitogen-activated protein kinases	834:866	mitogen-activated protein kinases (MAPKs) signaling pathways	834:893	The immunomodulatory effects of PTSO (0.1-25 µm) are also shown in vitro in Caco-2 and THP-1 cells, reducing the production of pro-inflammatory mediators and downregulating mitogen-activated protein kinases (MAPKs) signaling pathways.
30516875	4	28	theme	signaling	876:884	arg1	pathways					886:893	mitogen-activated protein kinases (MAPKs) signaling pathways	834:893	mitogen-activated protein kinases (MAPKs) signaling pathways	834:893	The immunomodulatory effects of PTSO (0.1-25 µm) are also shown in vitro in Caco-2 and THP-1 cells, reducing the production of pro-inflammatory mediators and downregulating mitogen-activated protein kinases (MAPKs) signaling pathways.
30516875	2	29	theme	bowel	407:411	arg1	diseases					413:420	inflammatory bowel diseases	394:420	inflammatory bowel diseases	394:420	In consequence, PTSO can be a potential candidate for the treatment of inflammatory bowel diseases.
30516875	1	30	theme	antimicrobial	297:309	arg1	properties					311:320	antioxidant, anti-inflammatory, immunomodulatory, and antimicrobial properties	243:320	antioxidant, anti-inflammatory, immunomodulatory, and antimicrobial properties	243:320	SCOPE Propyl-propane thiosulfonate (PTSO) is a component isolated from garlic (Allium sativum) with antioxidant, anti-inflammatory, immunomodulatory, and antimicrobial properties.
30516875	4	31	theme	mitogen-activated	834:850	arg1	MAPKs					869:873	MAPKs	869:873	MAPKs	869:873	The immunomodulatory effects of PTSO (0.1-25 µm) are also shown in vitro in Caco-2 and THP-1 cells, reducing the production of pro-inflammatory mediators and downregulating mitogen-activated protein kinases (MAPKs) signaling pathways.
30516875	4	31	theme	mitogen-activated	834:850	arg1	kinases					860:866	mitogen-activated protein kinases	834:866	mitogen-activated protein kinases (MAPKs) signaling pathways	834:893	The immunomodulatory effects of PTSO (0.1-25 µm) are also shown in vitro in Caco-2 and THP-1 cells, reducing the production of pro-inflammatory mediators and downregulating mitogen-activated protein kinases (MAPKs) signaling pathways.
30516875	6	32	theme	colitic	1178:1184	arg1	mice					1186:1189	DSS colitic mice	1174:1189	DSS colitic mice	1174:1189	Moreover, PTSO ameliorates the altered gut microbiota composition observed in DSS colitic mice.
30516875	2	33	theme	inflammatory	394:405	arg1	diseases					413:420	inflammatory bowel diseases	394:420	inflammatory bowel diseases	394:420	In consequence, PTSO can be a potential candidate for the treatment of inflammatory bowel diseases.
30516875	3	34	theme	PTSO	565:568	arg1	doses					570:574	PTSO doses	565:574	PTSO doses: 0.01-10 mg kg-1	565:591	METHODS AND RESULTS The anti-inflammatory effects of PTSO are studied in two mice models of colitis: 2,4-dinitrobenzene sulfonic acid (DNBS) (PTSO doses: 0.01-10 mg kg-1 ) and dextran sodium sulfate (DSS) (PTSO doses: 0.01-0.1 mg kg-1 ).
30516875	3	34	theme	PTSO	565:568	arg1	acid					552:555	2,4-dinitrobenzene sulfonic acid	524:555	2,4-dinitrobenzene sulfonic acid (DNBS) (PTSO doses: 0.01-10 mg kg-1 )	524:593	METHODS AND RESULTS The anti-inflammatory effects of PTSO are studied in two mice models of colitis: 2,4-dinitrobenzene sulfonic acid (DNBS) (PTSO doses: 0.01-10 mg kg-1 ) and dextran sodium sulfate (DSS) (PTSO doses: 0.01-0.1 mg kg-1 ).
30516875	5	35	theme	pro-inflammatory	1010:1025	arg1	mediators					1027:1035	different pro-inflammatory mediators	1000:1035	different pro-inflammatory mediators	1000:1035	This compound displays beneficial effects in both models of mouse colitis by reducing the expression of different pro-inflammatory mediators and improving the intestinal epithelial barrier integrity.
30516875	7	36	from	colitis	1269:1275	arg1	mice					1280:1283	mice	1280:1283	mice	1280:1283	CONCLUSION PTSO exerts intestinal anti-inflammatory activity in experimental colitis in mice.
30516875	6	37	located	observed	1162:1169	arg2	composition					1150:1160	the altered gut microbiota composition	1123:1160	the altered gut microbiota composition observed in DSS colitic mice	1123:1189	Moreover, PTSO ameliorates the altered gut microbiota composition observed in DSS colitic mice.
30516875	6	37	located	observed	1162:1169	arg1	mice					1186:1189	DSS colitic mice	1174:1189	DSS colitic mice	1174:1189	Moreover, PTSO ameliorates the altered gut microbiota composition observed in DSS colitic mice.
30516875	8	38	theme	inflammatory	1445:1456	arg1	response					1458:1465	the inflammatory response	1441:1465	the inflammatory response	1441:1465	This anti-inflammatory activity can be associated with the immunomodulatory properties of PTSO through the regulation of the activity of cells involved in the inflammatory response.
30516875	9	39	theme	future	1625:1630	arg1	development					1632:1642	its future development	1621:1642	its future development in human IBD	1621:1655	Furthermore, PTSO is able to restore the intestinal epithelial barrier function and to ameliorate the intestinal microbiota homeostasis, thus supporting its future development in human IBD.
30516875	1	40	attach	isolated	200:207	arg2	component					190:198	a component	188:198	a component isolated from garlic (Allium sativum) with antioxidant, anti-inflammatory, immunomodulatory, and antimicrobial properties	188:320	SCOPE Propyl-propane thiosulfonate (PTSO) is a component isolated from garlic (Allium sativum) with antioxidant, anti-inflammatory, immunomodulatory, and antimicrobial properties.
30516875	1	40	attach	isolated	200:207	arg1	garlic					214:219	garlic	214:219	garlic (Allium sativum) with antioxidant, anti-inflammatory, immunomodulatory, and antimicrobial properties	214:320	SCOPE Propyl-propane thiosulfonate (PTSO) is a component isolated from garlic (Allium sativum) with antioxidant, anti-inflammatory, immunomodulatory, and antimicrobial properties.
30516875	1	40	attach	isolated	200:207	arg2	thiosulfonate					164:176	SCOPE Propyl-propane thiosulfonate	143:176	SCOPE Propyl-propane thiosulfonate (PTSO)	143:183	SCOPE Propyl-propane thiosulfonate (PTSO) is a component isolated from garlic (Allium sativum) with antioxidant, anti-inflammatory, immunomodulatory, and antimicrobial properties.
30516875	1	40	attach	isolated	200:207	arg1	Allium					222:227	Allium	222:227	Allium	222:227	SCOPE Propyl-propane thiosulfonate (PTSO) is a component isolated from garlic (Allium sativum) with antioxidant, anti-inflammatory, immunomodulatory, and antimicrobial properties.
30516875	3	41	theme	colitis	515:521	arg1	models					505:510	two mice models	496:510	two mice models of colitis	496:521	METHODS AND RESULTS The anti-inflammatory effects of PTSO are studied in two mice models of colitis: 2,4-dinitrobenzene sulfonic acid (DNBS) (PTSO doses: 0.01-10 mg kg-1 ) and dextran sodium sulfate (DSS) (PTSO doses: 0.01-0.1 mg kg-1 ).
30516875	6	42	theme	altered	1127:1133	arg1	composition					1150:1160	the altered gut microbiota composition	1123:1160	the altered gut microbiota composition observed in DSS colitic mice	1123:1189	Moreover, PTSO ameliorates the altered gut microbiota composition observed in DSS colitic mice.
30516875	0	43	theme	Thiosulfonate	50:62	arg1	Properties					21:30	The Immunomodulatory Properties	0:30	The Immunomodulatory Properties of Propyl-Propane Thiosulfonate	0:62	The Immunomodulatory Properties of Propyl-Propane Thiosulfonate Contribute to its Intestinal Anti-Inflammatory Effect in Experimental Colitis.
30516875	2	44	theme	potential	353:361	arg1	candidate					363:371	a potential candidate	351:371	a potential candidate for the treatment of inflammatory bowel diseases	351:420	In consequence, PTSO can be a potential candidate for the treatment of inflammatory bowel diseases.
30516875	2	44	theme	potential	353:361	arg1	PTSO					339:342	PTSO	339:342	PTSO	339:342	In consequence, PTSO can be a potential candidate for the treatment of inflammatory bowel diseases.
30516875	3	45	theme	dextran	599:605	arg1	doses					634:638	PTSO doses	629:638	PTSO doses: 0.01-0.1 mg kg-1	629:656	METHODS AND RESULTS The anti-inflammatory effects of PTSO are studied in two mice models of colitis: 2,4-dinitrobenzene sulfonic acid (DNBS) (PTSO doses: 0.01-10 mg kg-1 ) and dextran sodium sulfate (DSS) (PTSO doses: 0.01-0.1 mg kg-1 ).
30516875	3	45	theme	dextran	599:605	arg1	DSS					623:625	DSS	623:625	DSS	623:625	METHODS AND RESULTS The anti-inflammatory effects of PTSO are studied in two mice models of colitis: 2,4-dinitrobenzene sulfonic acid (DNBS) (PTSO doses: 0.01-10 mg kg-1 ) and dextran sodium sulfate (DSS) (PTSO doses: 0.01-0.1 mg kg-1 ).
30516875	3	45	theme	dextran	599:605	arg1	METHODS					423:429	METHODS	423:429	METHODS	423:429	METHODS AND RESULTS The anti-inflammatory effects of PTSO are studied in two mice models of colitis: 2,4-dinitrobenzene sulfonic acid (DNBS) (PTSO doses: 0.01-10 mg kg-1 ) and dextran sodium sulfate (DSS) (PTSO doses: 0.01-0.1 mg kg-1 ).
30516875	3	45	theme	dextran	599:605	arg1	sulfate					614:620	dextran sodium sulfate	599:620	dextran sodium sulfate (DSS) (PTSO doses: 0.01-0.1 mg kg-1 )	599:658	METHODS AND RESULTS The anti-inflammatory effects of PTSO are studied in two mice models of colitis: 2,4-dinitrobenzene sulfonic acid (DNBS) (PTSO doses: 0.01-10 mg kg-1 ) and dextran sodium sulfate (DSS) (PTSO doses: 0.01-0.1 mg kg-1 ).
30516875	8	46	theme	anti-inflammatory	1291:1307	arg1	activity					1309:1316	This anti-inflammatory activity	1286:1316	This anti-inflammatory activity	1286:1316	This anti-inflammatory activity can be associated with the immunomodulatory properties of PTSO through the regulation of the activity of cells involved in the inflammatory response.
30516875	0	47	theme	Propyl-Propane	35:48	arg1	Thiosulfonate					50:62	Propyl-Propane Thiosulfonate	35:62	Propyl-Propane Thiosulfonate	35:62	The Immunomodulatory Properties of Propyl-Propane Thiosulfonate Contribute to its Intestinal Anti-Inflammatory Effect in Experimental Colitis.
30516875	1	48	theme	immunomodulatory	275:290	arg1	properties					311:320	antioxidant, anti-inflammatory, immunomodulatory, and antimicrobial properties	243:320	antioxidant, anti-inflammatory, immunomodulatory, and antimicrobial properties	243:320	SCOPE Propyl-propane thiosulfonate (PTSO) is a component isolated from garlic (Allium sativum) with antioxidant, anti-inflammatory, immunomodulatory, and antimicrobial properties.
30516875	8	49	theme	PTSO	1376:1379	arg1	properties					1362:1371	the immunomodulatory properties	1341:1371	the immunomodulatory properties of PTSO	1341:1379	This anti-inflammatory activity can be associated with the immunomodulatory properties of PTSO through the regulation of the activity of cells involved in the inflammatory response.
30516875	3	50	theme	sodium	607:612	arg1	doses					634:638	PTSO doses	629:638	PTSO doses: 0.01-0.1 mg kg-1	629:656	METHODS AND RESULTS The anti-inflammatory effects of PTSO are studied in two mice models of colitis: 2,4-dinitrobenzene sulfonic acid (DNBS) (PTSO doses: 0.01-10 mg kg-1 ) and dextran sodium sulfate (DSS) (PTSO doses: 0.01-0.1 mg kg-1 ).
30516875	3	50	theme	sodium	607:612	arg1	DSS					623:625	DSS	623:625	DSS	623:625	METHODS AND RESULTS The anti-inflammatory effects of PTSO are studied in two mice models of colitis: 2,4-dinitrobenzene sulfonic acid (DNBS) (PTSO doses: 0.01-10 mg kg-1 ) and dextran sodium sulfate (DSS) (PTSO doses: 0.01-0.1 mg kg-1 ).
30516875	3	50	theme	sodium	607:612	arg1	METHODS					423:429	METHODS	423:429	METHODS	423:429	METHODS AND RESULTS The anti-inflammatory effects of PTSO are studied in two mice models of colitis: 2,4-dinitrobenzene sulfonic acid (DNBS) (PTSO doses: 0.01-10 mg kg-1 ) and dextran sodium sulfate (DSS) (PTSO doses: 0.01-0.1 mg kg-1 ).
30516875	3	50	theme	sodium	607:612	arg1	sulfate					614:620	dextran sodium sulfate	599:620	dextran sodium sulfate (DSS) (PTSO doses: 0.01-0.1 mg kg-1 )	599:658	METHODS AND RESULTS The anti-inflammatory effects of PTSO are studied in two mice models of colitis: 2,4-dinitrobenzene sulfonic acid (DNBS) (PTSO doses: 0.01-10 mg kg-1 ) and dextran sodium sulfate (DSS) (PTSO doses: 0.01-0.1 mg kg-1 ).
30516875	3	51	theme	PTSO	629:632	arg1	doses					634:638	PTSO doses	629:638	PTSO doses: 0.01-0.1 mg kg-1	629:656	METHODS AND RESULTS The anti-inflammatory effects of PTSO are studied in two mice models of colitis: 2,4-dinitrobenzene sulfonic acid (DNBS) (PTSO doses: 0.01-10 mg kg-1 ) and dextran sodium sulfate (DSS) (PTSO doses: 0.01-0.1 mg kg-1 ).
30516875	3	51	theme	PTSO	629:632	arg1	sulfate					614:620	dextran sodium sulfate	599:620	dextran sodium sulfate (DSS) (PTSO doses: 0.01-0.1 mg kg-1 )	599:658	METHODS AND RESULTS The anti-inflammatory effects of PTSO are studied in two mice models of colitis: 2,4-dinitrobenzene sulfonic acid (DNBS) (PTSO doses: 0.01-10 mg kg-1 ) and dextran sodium sulfate (DSS) (PTSO doses: 0.01-0.1 mg kg-1 ).
30516875	4	52	theme	mediators	805:813	arg1	production					774:783	the production	770:783	the production of pro-inflammatory mediators	770:813	The immunomodulatory effects of PTSO (0.1-25 µm) are also shown in vitro in Caco-2 and THP-1 cells, reducing the production of pro-inflammatory mediators and downregulating mitogen-activated protein kinases (MAPKs) signaling pathways.
30516875	8	53	theme	cells	1423:1427	arg1	activity					1411:1418	the activity	1407:1418	the activity of cells involved in the inflammatory response	1407:1465	This anti-inflammatory activity can be associated with the immunomodulatory properties of PTSO through the regulation of the activity of cells involved in the inflammatory response.
30516875	1	54	theme	SCOPE	143:147	arg1	thiosulfonate					164:176	SCOPE Propyl-propane thiosulfonate	143:176	SCOPE Propyl-propane thiosulfonate (PTSO)	143:183	SCOPE Propyl-propane thiosulfonate (PTSO) is a component isolated from garlic (Allium sativum) with antioxidant, anti-inflammatory, immunomodulatory, and antimicrobial properties.
30516875	1	54	theme	SCOPE	143:147	arg1	component					190:198	a component	188:198	a component isolated from garlic (Allium sativum) with antioxidant, anti-inflammatory, immunomodulatory, and antimicrobial properties	188:320	SCOPE Propyl-propane thiosulfonate (PTSO) is a component isolated from garlic (Allium sativum) with antioxidant, anti-inflammatory, immunomodulatory, and antimicrobial properties.
30516875	1	54	theme	SCOPE	143:147	arg1	PTSO					179:182	PTSO	179:182	PTSO	179:182	SCOPE Propyl-propane thiosulfonate (PTSO) is a component isolated from garlic (Allium sativum) with antioxidant, anti-inflammatory, immunomodulatory, and antimicrobial properties.
30516875	9	55	theme	intestinal	1570:1579	arg1	homeostasis					1592:1602	the intestinal microbiota homeostasis	1566:1602	the intestinal microbiota homeostasis	1566:1602	Furthermore, PTSO is able to restore the intestinal epithelial barrier function and to ameliorate the intestinal microbiota homeostasis, thus supporting its future development in human IBD.
30516875	1	56	with	garlic	214:219	arg1	properties					311:320	antioxidant, anti-inflammatory, immunomodulatory, and antimicrobial properties	243:320	antioxidant, anti-inflammatory, immunomodulatory, and antimicrobial properties	243:320	SCOPE Propyl-propane thiosulfonate (PTSO) is a component isolated from garlic (Allium sativum) with antioxidant, anti-inflammatory, immunomodulatory, and antimicrobial properties.
30516875	4	57	theme	pro-inflammatory	788:803	arg1	mediators					805:813	pro-inflammatory mediators	788:813	pro-inflammatory mediators	788:813	The immunomodulatory effects of PTSO (0.1-25 µm) are also shown in vitro in Caco-2 and THP-1 cells, reducing the production of pro-inflammatory mediators and downregulating mitogen-activated protein kinases (MAPKs) signaling pathways.
30516875	1	58	theme	Propyl-propane	149:162	arg1	thiosulfonate					164:176	SCOPE Propyl-propane thiosulfonate	143:176	SCOPE Propyl-propane thiosulfonate (PTSO)	143:183	SCOPE Propyl-propane thiosulfonate (PTSO) is a component isolated from garlic (Allium sativum) with antioxidant, anti-inflammatory, immunomodulatory, and antimicrobial properties.
30516875	1	58	theme	Propyl-propane	149:162	arg1	component					190:198	a component	188:198	a component isolated from garlic (Allium sativum) with antioxidant, anti-inflammatory, immunomodulatory, and antimicrobial properties	188:320	SCOPE Propyl-propane thiosulfonate (PTSO) is a component isolated from garlic (Allium sativum) with antioxidant, anti-inflammatory, immunomodulatory, and antimicrobial properties.
30516875	1	58	theme	Propyl-propane	149:162	arg1	PTSO					179:182	PTSO	179:182	PTSO	179:182	SCOPE Propyl-propane thiosulfonate (PTSO) is a component isolated from garlic (Allium sativum) with antioxidant, anti-inflammatory, immunomodulatory, and antimicrobial properties.
30516875	5	59	theme	different	1000:1008	arg1	mediators					1027:1035	different pro-inflammatory mediators	1000:1035	different pro-inflammatory mediators	1000:1035	This compound displays beneficial effects in both models of mouse colitis by reducing the expression of different pro-inflammatory mediators and improving the intestinal epithelial barrier integrity.
30516875	5	60	theme	intestinal	1055:1064	arg1	integrity					1085:1093	the intestinal epithelial barrier integrity	1051:1093	the intestinal epithelial barrier integrity	1051:1093	This compound displays beneficial effects in both models of mouse colitis by reducing the expression of different pro-inflammatory mediators and improving the intestinal epithelial barrier integrity.
30516875	5	61	theme	mediators	1027:1035	arg1	expression					986:995	the expression	982:995	the expression of different pro-inflammatory mediators	982:1035	This compound displays beneficial effects in both models of mouse colitis by reducing the expression of different pro-inflammatory mediators and improving the intestinal epithelial barrier integrity.
30516875	3	62	theme	mice	500:503	arg1	models					505:510	two mice models	496:510	two mice models of colitis	496:521	METHODS AND RESULTS The anti-inflammatory effects of PTSO are studied in two mice models of colitis: 2,4-dinitrobenzene sulfonic acid (DNBS) (PTSO doses: 0.01-10 mg kg-1 ) and dextran sodium sulfate (DSS) (PTSO doses: 0.01-0.1 mg kg-1 ).
30516875	6	63	theme	DSS	1174:1176	arg1	mice					1186:1189	DSS colitic mice	1174:1189	DSS colitic mice	1174:1189	Moreover, PTSO ameliorates the altered gut microbiota composition observed in DSS colitic mice.
30516875	5	64	theme	beneficial	919:928	arg1	effects					930:936	beneficial effects	919:936	beneficial effects	919:936	This compound displays beneficial effects in both models of mouse colitis by reducing the expression of different pro-inflammatory mediators and improving the intestinal epithelial barrier integrity.
30516875	5	65	theme	epithelial	1066:1075	arg1	integrity					1085:1093	the intestinal epithelial barrier integrity	1051:1093	the intestinal epithelial barrier integrity	1051:1093	This compound displays beneficial effects in both models of mouse colitis by reducing the expression of different pro-inflammatory mediators and improving the intestinal epithelial barrier integrity.
30516875	9	66	dep	able	1489:1492	arg1	restore					1497:1503	restore	1497:1503	to restore the intestinal epithelial barrier function	1494:1546	Furthermore, PTSO is able to restore the intestinal epithelial barrier function and to ameliorate the intestinal microbiota homeostasis, thus supporting its future development in human IBD.
30516875	9	66	dep	able	1489:1492	arg1	ameliorate					1555:1564	ameliorate	1555:1564	to ameliorate the intestinal microbiota homeostasis	1552:1602	Furthermore, PTSO is able to restore the intestinal epithelial barrier function and to ameliorate the intestinal microbiota homeostasis, thus supporting its future development in human IBD.
30516875	4	67	theme	immunomodulatory	665:680	arg1	effects					682:688	The immunomodulatory effects	661:688	The immunomodulatory effects of PTSO (0.1-25 µm)	661:708	The immunomodulatory effects of PTSO (0.1-25 µm) are also shown in vitro in Caco-2 and THP-1 cells, reducing the production of pro-inflammatory mediators and downregulating mitogen-activated protein kinases (MAPKs) signaling pathways.
30516875	1	68	theme	antioxidant	243:253	arg1	properties					311:320	antioxidant, anti-inflammatory, immunomodulatory, and antimicrobial properties	243:320	antioxidant, anti-inflammatory, immunomodulatory, and antimicrobial properties	243:320	SCOPE Propyl-propane thiosulfonate (PTSO) is a component isolated from garlic (Allium sativum) with antioxidant, anti-inflammatory, immunomodulatory, and antimicrobial properties.
30516875	0	69	theme	Intestinal	82:91	arg1	Effect					111:116	its Intestinal Anti-Inflammatory Effect	78:116	its Intestinal Anti-Inflammatory Effect in Experimental Colitis	78:140	The Immunomodulatory Properties of Propyl-Propane Thiosulfonate Contribute to its Intestinal Anti-Inflammatory Effect in Experimental Colitis.
30516875	5	70	theme	barrier	1077:1083	arg1	integrity					1085:1093	the intestinal epithelial barrier integrity	1051:1093	the intestinal epithelial barrier integrity	1051:1093	This compound displays beneficial effects in both models of mouse colitis by reducing the expression of different pro-inflammatory mediators and improving the intestinal epithelial barrier integrity.
30516875	9	71	theme	microbiota	1581:1590	arg1	homeostasis					1592:1602	the intestinal microbiota homeostasis	1566:1602	the intestinal microbiota homeostasis	1566:1602	Furthermore, PTSO is able to restore the intestinal epithelial barrier function and to ameliorate the intestinal microbiota homeostasis, thus supporting its future development in human IBD.
30516875	3	72	theme	sulfonic	543:550	arg1	doses					570:574	PTSO doses	565:574	PTSO doses: 0.01-10 mg kg-1	565:591	METHODS AND RESULTS The anti-inflammatory effects of PTSO are studied in two mice models of colitis: 2,4-dinitrobenzene sulfonic acid (DNBS) (PTSO doses: 0.01-10 mg kg-1 ) and dextran sodium sulfate (DSS) (PTSO doses: 0.01-0.1 mg kg-1 ).
30516875	3	72	theme	sulfonic	543:550	arg1	DNBS					558:561	DNBS	558:561	DNBS	558:561	METHODS AND RESULTS The anti-inflammatory effects of PTSO are studied in two mice models of colitis: 2,4-dinitrobenzene sulfonic acid (DNBS) (PTSO doses: 0.01-10 mg kg-1 ) and dextran sodium sulfate (DSS) (PTSO doses: 0.01-0.1 mg kg-1 ).
30516875	3	72	theme	sulfonic	543:550	arg1	METHODS					423:429	METHODS	423:429	METHODS	423:429	METHODS AND RESULTS The anti-inflammatory effects of PTSO are studied in two mice models of colitis: 2,4-dinitrobenzene sulfonic acid (DNBS) (PTSO doses: 0.01-10 mg kg-1 ) and dextran sodium sulfate (DSS) (PTSO doses: 0.01-0.1 mg kg-1 ).
30516875	3	72	theme	sulfonic	543:550	arg1	acid					552:555	2,4-dinitrobenzene sulfonic acid	524:555	2,4-dinitrobenzene sulfonic acid (DNBS) (PTSO doses: 0.01-10 mg kg-1 )	524:593	METHODS AND RESULTS The anti-inflammatory effects of PTSO are studied in two mice models of colitis: 2,4-dinitrobenzene sulfonic acid (DNBS) (PTSO doses: 0.01-10 mg kg-1 ) and dextran sodium sulfate (DSS) (PTSO doses: 0.01-0.1 mg kg-1 ).
30516875	3	73	theme	PTSO	476:479	arg1	effects					465:471	The anti-inflammatory effects	443:471	The anti-inflammatory effects of PTSO	443:479	METHODS AND RESULTS The anti-inflammatory effects of PTSO are studied in two mice models of colitis: 2,4-dinitrobenzene sulfonic acid (DNBS) (PTSO doses: 0.01-10 mg kg-1 ) and dextran sodium sulfate (DSS) (PTSO doses: 0.01-0.1 mg kg-1 ).
30516875	3	74	dep	doses	634:638	arg1	0.01-0.1 mg kg-1					641:656	0.01-0.1 mg kg-1	641:656	PTSO doses: 0.01-0.1 mg kg-1	629:656	METHODS AND RESULTS The anti-inflammatory effects of PTSO are studied in two mice models of colitis: 2,4-dinitrobenzene sulfonic acid (DNBS) (PTSO doses: 0.01-10 mg kg-1 ) and dextran sodium sulfate (DSS) (PTSO doses: 0.01-0.1 mg kg-1 ).
30516875	1	75	dep	Allium	222:227	arg1	sativum					229:235	Allium sativum	222:235	Allium sativum	222:235	SCOPE Propyl-propane thiosulfonate (PTSO) is a component isolated from garlic (Allium sativum) with antioxidant, anti-inflammatory, immunomodulatory, and antimicrobial properties.
30516875	3	76	dep	doses	570:574	arg1	0.01-10 mg kg-1					577:591	0.01-10 mg kg-1	577:591	PTSO doses: 0.01-10 mg kg-1	565:591	METHODS AND RESULTS The anti-inflammatory effects of PTSO are studied in two mice models of colitis: 2,4-dinitrobenzene sulfonic acid (DNBS) (PTSO doses: 0.01-10 mg kg-1 ) and dextran sodium sulfate (DSS) (PTSO doses: 0.01-0.1 mg kg-1 ).
30516875	1	77	theme	anti-inflammatory	256:272	arg1	properties					311:320	antioxidant, anti-inflammatory, immunomodulatory, and antimicrobial properties	243:320	antioxidant, anti-inflammatory, immunomodulatory, and antimicrobial properties	243:320	SCOPE Propyl-propane thiosulfonate (PTSO) is a component isolated from garlic (Allium sativum) with antioxidant, anti-inflammatory, immunomodulatory, and antimicrobial properties.
29703739	14	0	theme	genome	1267:1272	arg1	sequence					1274:1281	the genome sequence	1263:1281	the genome sequence	1263:1281	longum We compared the genome sequence and growth and transcriptional characteristics of an OTU49 isolate cultured in HMO medium to those of other B. longum subsp.
29703739	17	1	theme	High-throughput	1790:1804	arg1	methods					1821:1827	ecological functions.IMPORTANCE High-throughput DNA sequencing methods	1758:1827	ecological functions.IMPORTANCE High-throughput DNA sequencing methods	1758:1827	infantis, that dependable quantitative PCR (qPCR) differentiation between the B. longum subspecies cannot be achieved by targeting tuf gene sequences, and that functional genes involved in carbohydrate metabolism might be better targets because they delineate ecological functions.IMPORTANCE High-throughput DNA sequencing methods and advanced bioinformatics analysis have revealed the composition and biochemical capacities of microbial communities (microbiota and microbiome), including those that inhabit the gut of human infants.
29703739	18	2	theme	microbiota	2185:2194	arg1	interactions					2196:2207	gut microbiota interactions	2181:2207	gut microbiota interactions	2181:2207	However, the microbiology and function of natural ecosystems have received little attention in recent decades, so an appreciation of the dynamics of gut microbiota interactions is lacking.
29703739	19	3	dep	prevalences	2321:2331	arg1	the					2317:2319	the	2317:2319	the	2317:2319	With respect to infants, rapid methodologies, such as quantitative PCR, are needed to determine the prevalences and proportions of different bifidobacterial species in observational and microcosm studies in order to obtain a better understanding of the dynamics of bifidobacterial nutrition and syntrophy, knowledge that might be used to manipulate the microbiota and perhaps ensure the better health of infants.
29703739	2	4	theme	genus	148:152	arg1	Bifidobacterium					154:168	the bacterial genus Bifidobacterium	134:168	the bacterial genus Bifidobacterium	134:168	Members of the bacterial genus Bifidobacterium generally dominate the fecal microbiota of infants.
29703739	12	5	theme	gene	1086:1089	arg1	variant					1091:1097	a tuf gene variant	1080:1097	a tuf gene variant	1080:1097	This approach resulted in the detection of a tuf gene variant (operational taxonomic unit 49 [OTU49]) in Chinese infants that has sequence similarities to both B. longum subsp.
29703739	17	6	theme	human	2017:2021	arg1	infants					2023:2029	human infants	2017:2029	human infants	2017:2029	infantis, that dependable quantitative PCR (qPCR) differentiation between the B. longum subspecies cannot be achieved by targeting tuf gene sequences, and that functional genes involved in carbohydrate metabolism might be better targets because they delineate ecological functions.IMPORTANCE High-throughput DNA sequencing methods and advanced bioinformatics analysis have revealed the composition and biochemical capacities of microbial communities (microbiota and microbiome), including those that inhabit the gut of human infants.
29703739	1	7	theme	Chinese	106:112	arg1	Infants					114:120	Chinese Infants	106:120	Chinese Infants	106:120	infantis Detected in the Fecal Microbiota of Chinese Infants.
29703739	19	8	theme	species	2378:2384	arg1	proportions					2337:2347	proportions	2337:2347	proportions	2337:2347	With respect to infants, rapid methodologies, such as quantitative PCR, are needed to determine the prevalences and proportions of different bifidobacterial species in observational and microcosm studies in order to obtain a better understanding of the dynamics of bifidobacterial nutrition and syntrophy, knowledge that might be used to manipulate the microbiota and perhaps ensure the better health of infants.
29703739	19	8	theme	species	2378:2384	arg1	prevalences					2321:2331	prevalences	2321:2331	prevalences	2321:2331	With respect to infants, rapid methodologies, such as quantitative PCR, are needed to determine the prevalences and proportions of different bifidobacterial species in observational and microcosm studies in order to obtain a better understanding of the dynamics of bifidobacterial nutrition and syntrophy, knowledge that might be used to manipulate the microbiota and perhaps ensure the better health of infants.
29703739	17	9	theme	communities	1936:1946	arg1	composition					1884:1894	composition	1884:1894	composition	1884:1894	infantis, that dependable quantitative PCR (qPCR) differentiation between the B. longum subspecies cannot be achieved by targeting tuf gene sequences, and that functional genes involved in carbohydrate metabolism might be better targets because they delineate ecological functions.IMPORTANCE High-throughput DNA sequencing methods and advanced bioinformatics analysis have revealed the composition and biochemical capacities of microbial communities (microbiota and microbiome), including those that inhabit the gut of human infants.
29703739	17	9	theme	communities	1936:1946	arg1	capacities					1912:1921	biochemical capacities	1900:1921	biochemical capacities	1900:1921	infantis, that dependable quantitative PCR (qPCR) differentiation between the B. longum subspecies cannot be achieved by targeting tuf gene sequences, and that functional genes involved in carbohydrate metabolism might be better targets because they delineate ecological functions.IMPORTANCE High-throughput DNA sequencing methods and advanced bioinformatics analysis have revealed the composition and biochemical capacities of microbial communities (microbiota and microbiome), including those that inhabit the gut of human infants.
29703739	16	10	dep	B.	1481:1482	arg1	longum					1484:1489	longum	1484:1489	longum	1484:1489	We concluded from these studies that OTU49 belongs to B. longum subsp.
29703739	15	11	theme	infantis	1408:1415	arg1	cultures					1417:1424	infantis cultures	1408:1424	infantis cultures	1408:1424	infantis cultures.
29703739	13	12	dep	B.	1227:1228	arg1	longum					1230:1235	longum	1230:1235	longum	1230:1235	infantis and B. longum subsp.
29703739	3	13	theme	B.	279:280	arg1	subsp					289:293	the B. longum subsp	275:293	the B. longum subsp	275:293	The species Bifidobacterium longum is prevalent, but the B. longum subsp.
29703739	14	14	theme	B.	1391:1392	arg1	subsp					1401:1405	other B. longum subsp	1385:1405	other B. longum subsp	1385:1405	longum We compared the genome sequence and growth and transcriptional characteristics of an OTU49 isolate cultured in HMO medium to those of other B. longum subsp.
29703739	7	15	theme	target	706:711	arg1	species					713:719	target species	706:719	target species	706:719	Quantitative PCR provides a quick analytical method by which to accurately ascertain the abundances of target species in microbiotas and microcosms.
29703739	17	16	theme	PCR	1537:1539	arg1	differentiation					1548:1562	dependable quantitative PCR (qPCR) differentiation	1513:1562	dependable quantitative PCR (qPCR) differentiation between the B. longum subspecies	1513:1595	infantis, that dependable quantitative PCR (qPCR) differentiation between the B. longum subspecies cannot be achieved by targeting tuf gene sequences, and that functional genes involved in carbohydrate metabolism might be better targets because they delineate ecological functions.IMPORTANCE High-throughput DNA sequencing methods and advanced bioinformatics analysis have revealed the composition and biochemical capacities of microbial communities (microbiota and microbiome), including those that inhabit the gut of human infants.
29703739	6	17	theme	symbiotic	576:584	arg1	roles					586:590	different ecological and symbiotic roles	551:590	different ecological and symbiotic roles	551:590	These subspecies differ in their capacities to metabolize human milk oligosaccharides (HMO) and may have different ecological and symbiotic roles in humans.
29703739	17	18	dep	B.	1576:1577	arg1	longum					1579:1584	longum	1579:1584	longum	1579:1584	infantis, that dependable quantitative PCR (qPCR) differentiation between the B. longum subspecies cannot be achieved by targeting tuf gene sequences, and that functional genes involved in carbohydrate metabolism might be better targets because they delineate ecological functions.IMPORTANCE High-throughput DNA sequencing methods and advanced bioinformatics analysis have revealed the composition and biochemical capacities of microbial communities (microbiota and microbiome), including those that inhabit the gut of human infants.
29703739	6	19	theme	ecological	561:570	arg1	roles					586:590	different ecological and symbiotic roles	551:590	different ecological and symbiotic roles	551:590	These subspecies differ in their capacities to metabolize human milk oligosaccharides (HMO) and may have different ecological and symbiotic roles in humans.
29703739	8	20	theme	amplification	761:773	arg1	targets					775:781	amplification targets	761:781	amplification targets in DNA extracted from samples	761:811	However, amplification targets in DNA extracted from samples need to be dependably differential.
29703739	6	21	theme	milk	510:513	arg1	HMO					533:535	HMO	533:535	HMO	533:535	These subspecies differ in their capacities to metabolize human milk oligosaccharides (HMO) and may have different ecological and symbiotic roles in humans.
29703739	6	21	theme	milk	510:513	arg1	oligosaccharides					515:530	human milk oligosaccharides	504:530	human milk oligosaccharides (HMO)	504:536	These subspecies differ in their capacities to metabolize human milk oligosaccharides (HMO) and may have different ecological and symbiotic roles in humans.
29703739	1	22	theme	Infants	114:120	arg1	Microbiota					92:101	the Fecal Microbiota	82:101	the Fecal Microbiota of Chinese Infants	82:120	infantis Detected in the Fecal Microbiota of Chinese Infants.
29703739	17	23	theme	tuf	1629:1631	arg1	sequences					1638:1646	tuf gene sequences	1629:1646	tuf gene sequences	1629:1646	infantis, that dependable quantitative PCR (qPCR) differentiation between the B. longum subspecies cannot be achieved by targeting tuf gene sequences, and that functional genes involved in carbohydrate metabolism might be better targets because they delineate ecological functions.IMPORTANCE High-throughput DNA sequencing methods and advanced bioinformatics analysis have revealed the composition and biochemical capacities of microbial communities (microbiota and microbiome), including those that inhabit the gut of human infants.
29703739	19	24	theme	different	2352:2360	arg1	species					2378:2384	different bifidobacterial species	2352:2384	different bifidobacterial species	2352:2384	With respect to infants, rapid methodologies, such as quantitative PCR, are needed to determine the prevalences and proportions of different bifidobacterial species in observational and microcosm studies in order to obtain a better understanding of the dynamics of bifidobacterial nutrition and syntrophy, knowledge that might be used to manipulate the microbiota and perhaps ensure the better health of infants.
29703739	19	25	theme	better	2608:2613	arg1	health					2615:2620	the better health	2604:2620	the better health of infants	2604:2631	With respect to infants, rapid methodologies, such as quantitative PCR, are needed to determine the prevalences and proportions of different bifidobacterial species in observational and microcosm studies in order to obtain a better understanding of the dynamics of bifidobacterial nutrition and syntrophy, knowledge that might be used to manipulate the microbiota and perhaps ensure the better health of infants.
29703739	19	26	theme	rapid	2246:2250	arg1	PCR					2288:2290	quantitative PCR	2275:2290	quantitative PCR	2275:2290	With respect to infants, rapid methodologies, such as quantitative PCR, are needed to determine the prevalences and proportions of different bifidobacterial species in observational and microcosm studies in order to obtain a better understanding of the dynamics of bifidobacterial nutrition and syntrophy, knowledge that might be used to manipulate the microbiota and perhaps ensure the better health of infants.
29703739	19	26	theme	rapid	2246:2250	arg1	methodologies					2252:2264	rapid methodologies	2246:2264	rapid methodologies	2246:2264	With respect to infants, rapid methodologies, such as quantitative PCR, are needed to determine the prevalences and proportions of different bifidobacterial species in observational and microcosm studies in order to obtain a better understanding of the dynamics of bifidobacterial nutrition and syntrophy, knowledge that might be used to manipulate the microbiota and perhaps ensure the better health of infants.
29703739	7	27	theme	analytical	637:646	arg1	method					648:653	a quick analytical method	629:653	a quick analytical method by which to accurately ascertain the abundances of target species in microbiotas and microcosms	629:749	Quantitative PCR provides a quick analytical method by which to accurately ascertain the abundances of target species in microbiotas and microcosms.
29703739	7	28	from	abundances	692:701	arg1	microcosms					740:749	microcosms	740:749	microcosms	740:749	Quantitative PCR provides a quick analytical method by which to accurately ascertain the abundances of target species in microbiotas and microcosms.
29703739	7	28	from	abundances	692:701	arg1	microbiotas					724:734	microbiotas	724:734	microbiotas	724:734	Quantitative PCR provides a quick analytical method by which to accurately ascertain the abundances of target species in microbiotas and microcosms.
29703739	7	29	theme	Quantitative	603:614	arg1	PCR					616:618	Quantitative PCR	603:618	Quantitative PCR	603:618	Quantitative PCR provides a quick analytical method by which to accurately ascertain the abundances of target species in microbiotas and microcosms.
29703739	14	30	theme	isolate	1342:1348	arg1	growth					1287:1292	growth	1287:1292	growth	1287:1292	longum We compared the genome sequence and growth and transcriptional characteristics of an OTU49 isolate cultured in HMO medium to those of other B. longum subsp.
29703739	14	30	theme	isolate	1342:1348	arg1	characteristics					1314:1328	transcriptional characteristics	1298:1328	transcriptional characteristics	1298:1328	longum We compared the genome sequence and growth and transcriptional characteristics of an OTU49 isolate cultured in HMO medium to those of other B. longum subsp.
29703739	14	30	theme	isolate	1342:1348	arg1	sequence					1274:1281	the genome sequence	1263:1281	the genome sequence	1263:1281	longum We compared the genome sequence and growth and transcriptional characteristics of an OTU49 isolate cultured in HMO medium to those of other B. longum subsp.
29703739	16	31	theme	B.	1481:1482	arg1	subsp					1491:1495	B. longum subsp	1481:1495	B. longum subsp	1481:1495	We concluded from these studies that OTU49 belongs to B. longum subsp.
29703739	17	32	theme	B.	1576:1577	arg1	subspecies					1586:1595	the B. longum subspecies	1572:1595	the B. longum subspecies	1572:1595	infantis, that dependable quantitative PCR (qPCR) differentiation between the B. longum subspecies cannot be achieved by targeting tuf gene sequences, and that functional genes involved in carbohydrate metabolism might be better targets because they delineate ecological functions.IMPORTANCE High-throughput DNA sequencing methods and advanced bioinformatics analysis have revealed the composition and biochemical capacities of microbial communities (microbiota and microbiome), including those that inhabit the gut of human infants.
29703739	10	33	dep	B.	991:992	arg1	longum					994:999	longum	994:999	longum	994:999	infantis and B. longum subsp.
29703739	19	34	used	used	2551:2554	arg2	knowledge					2527:2535	knowledge	2527:2535	knowledge that might be used to manipulate the microbiota and perhaps ensure the better health of infants	2527:2631	With respect to infants, rapid methodologies, such as quantitative PCR, are needed to determine the prevalences and proportions of different bifidobacterial species in observational and microcosm studies in order to obtain a better understanding of the dynamics of bifidobacterial nutrition and syntrophy, knowledge that might be used to manipulate the microbiota and perhaps ensure the better health of infants.
29703739	19	34	used	used	2551:2554	arg2	dynamics					2474:2481	the dynamics	2470:2481	the dynamics of bifidobacterial nutrition and syntrophy	2470:2524	With respect to infants, rapid methodologies, such as quantitative PCR, are needed to determine the prevalences and proportions of different bifidobacterial species in observational and microcosm studies in order to obtain a better understanding of the dynamics of bifidobacterial nutrition and syntrophy, knowledge that might be used to manipulate the microbiota and perhaps ensure the better health of infants.
29703739	14	35	dep	B.	1391:1392	arg1	longum					1394:1399	longum	1394:1399	longum	1394:1399	longum We compared the genome sequence and growth and transcriptional characteristics of an OTU49 isolate cultured in HMO medium to those of other B. longum subsp.
29703739	0	36	theme	tuf	0:2	arg1	Variation					18:26	tuf Gene Sequence Variation	0:26	tuf Gene Sequence Variation in Bifidobacterium longum subsp	0:58	tuf Gene Sequence Variation in Bifidobacterium longum subsp.
29703739	19	37	theme	better	2446:2451	arg1	understanding					2453:2465	a better understanding	2444:2465	a better understanding of the dynamics of bifidobacterial nutrition and syntrophy, knowledge that might be used to manipulate the microbiota and perhaps ensure the better health of infants	2444:2631	With respect to infants, rapid methodologies, such as quantitative PCR, are needed to determine the prevalences and proportions of different bifidobacterial species in observational and microcosm studies in order to obtain a better understanding of the dynamics of bifidobacterial nutrition and syntrophy, knowledge that might be used to manipulate the microbiota and perhaps ensure the better health of infants.
29703739	17	38	theme	microbial	1926:1934	arg1	microbiota					1949:1958	microbiota	1949:1958	microbiota	1949:1958	infantis, that dependable quantitative PCR (qPCR) differentiation between the B. longum subspecies cannot be achieved by targeting tuf gene sequences, and that functional genes involved in carbohydrate metabolism might be better targets because they delineate ecological functions.IMPORTANCE High-throughput DNA sequencing methods and advanced bioinformatics analysis have revealed the composition and biochemical capacities of microbial communities (microbiota and microbiome), including those that inhabit the gut of human infants.
29703739	17	38	theme	microbial	1926:1934	arg1	communities					1936:1946	microbial communities	1926:1946	microbial communities (microbiota and microbiome)	1926:1974	infantis, that dependable quantitative PCR (qPCR) differentiation between the B. longum subspecies cannot be achieved by targeting tuf gene sequences, and that functional genes involved in carbohydrate metabolism might be better targets because they delineate ecological functions.IMPORTANCE High-throughput DNA sequencing methods and advanced bioinformatics analysis have revealed the composition and biochemical capacities of microbial communities (microbiota and microbiome), including those that inhabit the gut of human infants.
29703739	17	38	theme	microbial	1926:1934	arg1	those					1987:1991	those	1987:1991	those	1987:1991	infantis, that dependable quantitative PCR (qPCR) differentiation between the B. longum subspecies cannot be achieved by targeting tuf gene sequences, and that functional genes involved in carbohydrate metabolism might be better targets because they delineate ecological functions.IMPORTANCE High-throughput DNA sequencing methods and advanced bioinformatics analysis have revealed the composition and biochemical capacities of microbial communities (microbiota and microbiome), including those that inhabit the gut of human infants.
29703739	17	38	theme	microbial	1926:1934	arg1	microbiome					1964:1973	microbiome	1964:1973	microbiome	1964:1973	infantis, that dependable quantitative PCR (qPCR) differentiation between the B. longum subspecies cannot be achieved by targeting tuf gene sequences, and that functional genes involved in carbohydrate metabolism might be better targets because they delineate ecological functions.IMPORTANCE High-throughput DNA sequencing methods and advanced bioinformatics analysis have revealed the composition and biochemical capacities of microbial communities (microbiota and microbiome), including those that inhabit the gut of human infants.
29703739	1	39	theme	Fecal	86:90	arg1	Microbiota					92:101	the Fecal Microbiota	82:101	the Fecal Microbiota of Chinese Infants	82:120	infantis Detected in the Fecal Microbiota of Chinese Infants.
29703739	9	40	theme	gene	870:873	arg1	target					899:904	a molecular target	887:904	a molecular target for quantitative PCR measurements of the abundances of B. longum subsp	887:975	We evaluated the tuf gene sequence as a molecular target for quantitative PCR measurements of the abundances of B. longum subsp.
29703739	9	40	theme	gene	870:873	arg1	sequence					875:882	the tuf gene sequence	862:882	the tuf gene sequence	862:882	We evaluated the tuf gene sequence as a molecular target for quantitative PCR measurements of the abundances of B. longum subsp.
29703739	0	41	theme	Sequence	9:16	arg1	Variation					18:26	tuf Gene Sequence Variation	0:26	tuf Gene Sequence Variation in Bifidobacterium longum subsp	0:58	tuf Gene Sequence Variation in Bifidobacterium longum subsp.
29703739	17	42	theme	sequencing	1810:1819	arg1	methods					1821:1827	ecological functions.IMPORTANCE High-throughput DNA sequencing methods	1758:1827	ecological functions.IMPORTANCE High-throughput DNA sequencing methods	1758:1827	infantis, that dependable quantitative PCR (qPCR) differentiation between the B. longum subspecies cannot be achieved by targeting tuf gene sequences, and that functional genes involved in carbohydrate metabolism might be better targets because they delineate ecological functions.IMPORTANCE High-throughput DNA sequencing methods and advanced bioinformatics analysis have revealed the composition and biochemical capacities of microbial communities (microbiota and microbiome), including those that inhabit the gut of human infants.
29703739	19	43	theme	dynamics	2474:2481	arg1	understanding					2453:2465	a better understanding	2444:2465	a better understanding of the dynamics of bifidobacterial nutrition and syntrophy, knowledge that might be used to manipulate the microbiota and perhaps ensure the better health of infants	2444:2631	With respect to infants, rapid methodologies, such as quantitative PCR, are needed to determine the prevalences and proportions of different bifidobacterial species in observational and microcosm studies in order to obtain a better understanding of the dynamics of bifidobacterial nutrition and syntrophy, knowledge that might be used to manipulate the microbiota and perhaps ensure the better health of infants.
29703739	18	44	theme	ecosystems	2082:2091	arg1	function					2062:2069	function	2062:2069	function	2062:2069	However, the microbiology and function of natural ecosystems have received little attention in recent decades, so an appreciation of the dynamics of gut microbiota interactions is lacking.
29703739	18	44	theme	ecosystems	2082:2091	arg1	microbiology					2045:2056	microbiology	2045:2056	microbiology	2045:2056	However, the microbiology and function of natural ecosystems have received little attention in recent decades, so an appreciation of the dynamics of gut microbiota interactions is lacking.
29703739	17	45	theme	better	1720:1725	arg1	targets					1727:1733	better targets	1720:1733	better targets	1720:1733	infantis, that dependable quantitative PCR (qPCR) differentiation between the B. longum subspecies cannot be achieved by targeting tuf gene sequences, and that functional genes involved in carbohydrate metabolism might be better targets because they delineate ecological functions.IMPORTANCE High-throughput DNA sequencing methods and advanced bioinformatics analysis have revealed the composition and biochemical capacities of microbial communities (microbiota and microbiome), including those that inhabit the gut of human infants.
29703739	9	46	theme	molecular	889:897	arg1	target					899:904	a molecular target	887:904	a molecular target for quantitative PCR measurements of the abundances of B. longum subsp	887:975	We evaluated the tuf gene sequence as a molecular target for quantitative PCR measurements of the abundances of B. longum subsp.
29703739	9	46	theme	molecular	889:897	arg1	sequence					875:882	the tuf gene sequence	862:882	the tuf gene sequence	862:882	We evaluated the tuf gene sequence as a molecular target for quantitative PCR measurements of the abundances of B. longum subsp.
29703739	2	47	theme	Bifidobacterium	154:168	arg1	Members					123:129	Members	123:129	Members of the bacterial genus Bifidobacterium	123:168	Members of the bacterial genus Bifidobacterium generally dominate the fecal microbiota of infants.
29703739	0	48	theme	longum	47:52	arg1	subsp					54:58	Bifidobacterium longum subsp	31:58	Bifidobacterium longum subsp	31:58	tuf Gene Sequence Variation in Bifidobacterium longum subsp.
29703739	17	49	theme	bioinformatics	1842:1855	arg1	analysis					1857:1864	advanced bioinformatics analysis	1833:1864	advanced bioinformatics analysis	1833:1864	infantis, that dependable quantitative PCR (qPCR) differentiation between the B. longum subspecies cannot be achieved by targeting tuf gene sequences, and that functional genes involved in carbohydrate metabolism might be better targets because they delineate ecological functions.IMPORTANCE High-throughput DNA sequencing methods and advanced bioinformatics analysis have revealed the composition and biochemical capacities of microbial communities (microbiota and microbiome), including those that inhabit the gut of human infants.
29703739	18	50	theme	recent	2127:2132	arg1	decades					2134:2140	recent decades	2127:2140	recent decades	2127:2140	However, the microbiology and function of natural ecosystems have received little attention in recent decades, so an appreciation of the dynamics of gut microbiota interactions is lacking.
29703739	19	51	dep	infants	2237:2243	arg1	respect					2226:2232	respect	2226:2232	respect	2226:2232	With respect to infants, rapid methodologies, such as quantitative PCR, are needed to determine the prevalences and proportions of different bifidobacterial species in observational and microcosm studies in order to obtain a better understanding of the dynamics of bifidobacterial nutrition and syntrophy, knowledge that might be used to manipulate the microbiota and perhaps ensure the better health of infants.
29703739	9	52	theme	PCR	923:925	arg1	measurements					927:938	quantitative PCR measurements	910:938	quantitative PCR measurements of the abundances of B. longum subsp	910:975	We evaluated the tuf gene sequence as a molecular target for quantitative PCR measurements of the abundances of B. longum subsp.
29703739	2	53	theme	fecal	193:197	arg1	microbiota					199:208	the fecal microbiota	189:208	the fecal microbiota of infants	189:219	Members of the bacterial genus Bifidobacterium generally dominate the fecal microbiota of infants.
29703739	19	54	theme	bifidobacterial	2486:2500	arg1	nutrition					2502:2510	bifidobacterial nutrition	2486:2510	bifidobacterial nutrition	2486:2510	With respect to infants, rapid methodologies, such as quantitative PCR, are needed to determine the prevalences and proportions of different bifidobacterial species in observational and microcosm studies in order to obtain a better understanding of the dynamics of bifidobacterial nutrition and syntrophy, knowledge that might be used to manipulate the microbiota and perhaps ensure the better health of infants.
29703739	10	55	theme	B.	991:992	arg1	subsp					1001:1005	B. longum subsp	991:1005	B. longum subsp	991:1005	infantis and B. longum subsp.
29703739	17	56	theme	functions.IMPORTANCE	1769:1788	arg1	methods					1821:1827	ecological functions.IMPORTANCE High-throughput DNA sequencing methods	1758:1827	ecological functions.IMPORTANCE High-throughput DNA sequencing methods	1758:1827	infantis, that dependable quantitative PCR (qPCR) differentiation between the B. longum subspecies cannot be achieved by targeting tuf gene sequences, and that functional genes involved in carbohydrate metabolism might be better targets because they delineate ecological functions.IMPORTANCE High-throughput DNA sequencing methods and advanced bioinformatics analysis have revealed the composition and biochemical capacities of microbial communities (microbiota and microbiome), including those that inhabit the gut of human infants.
29703739	3	57	dep	B.	279:280	arg1	longum					282:287	longum	282:287	longum	282:287	The species Bifidobacterium longum is prevalent, but the B. longum subsp.
29703739	12	58	theme	tuf	1082:1084	arg1	variant					1091:1097	a tuf gene variant	1080:1097	a tuf gene variant	1080:1097	This approach resulted in the detection of a tuf gene variant (operational taxonomic unit 49 [OTU49]) in Chinese infants that has sequence similarities to both B. longum subsp.
29703739	17	59	theme	advanced	1833:1840	arg1	analysis					1857:1864	advanced bioinformatics analysis	1833:1864	advanced bioinformatics analysis	1833:1864	infantis, that dependable quantitative PCR (qPCR) differentiation between the B. longum subspecies cannot be achieved by targeting tuf gene sequences, and that functional genes involved in carbohydrate metabolism might be better targets because they delineate ecological functions.IMPORTANCE High-throughput DNA sequencing methods and advanced bioinformatics analysis have revealed the composition and biochemical capacities of microbial communities (microbiota and microbiome), including those that inhabit the gut of human infants.
29703739	17	60	theme	functional	1658:1667	arg1	genes					1669:1673	functional genes	1658:1673	functional genes involved in carbohydrate metabolism	1658:1709	infantis, that dependable quantitative PCR (qPCR) differentiation between the B. longum subspecies cannot be achieved by targeting tuf gene sequences, and that functional genes involved in carbohydrate metabolism might be better targets because they delineate ecological functions.IMPORTANCE High-throughput DNA sequencing methods and advanced bioinformatics analysis have revealed the composition and biochemical capacities of microbial communities (microbiota and microbiome), including those that inhabit the gut of human infants.
29703739	9	61	theme	abundances	947:956	arg1	measurements					927:938	quantitative PCR measurements	910:938	quantitative PCR measurements of the abundances of B. longum subsp	910:975	We evaluated the tuf gene sequence as a molecular target for quantitative PCR measurements of the abundances of B. longum subsp.
29703739	13	62	theme	B.	1227:1228	arg1	subsp					1237:1241	B. longum subsp	1227:1241	B. longum subsp	1227:1241	infantis and B. longum subsp.
29703739	12	63	theme	variant	1091:1097	arg1	detection					1067:1075	the detection	1063:1075	the detection of a tuf gene variant	1063:1097	This approach resulted in the detection of a tuf gene variant (operational taxonomic unit 49 [OTU49]) in Chinese infants that has sequence similarities to both B. longum subsp.
29703739	5	64	theme	microbiota	419:428	arg1	investigations					430:443	microbiota investigations	419:443	microbiota investigations	419:443	infantis strains that are known to colonize the infant bowel are not usually differentiated in microbiota investigations.
29703739	9	65	theme	B.	961:962	arg1	subsp					971:975	B. longum subsp	961:975	B. longum subsp	961:975	We evaluated the tuf gene sequence as a molecular target for quantitative PCR measurements of the abundances of B. longum subsp.
29703739	2	66	theme	bacterial	138:146	arg1	Bifidobacterium					154:168	the bacterial genus Bifidobacterium	134:168	the bacterial genus Bifidobacterium	134:168	Members of the bacterial genus Bifidobacterium generally dominate the fecal microbiota of infants.
29703739	12	67	dep	B.	1197:1198	arg1	longum					1200:1205	longum	1200:1205	longum	1200:1205	This approach resulted in the detection of a tuf gene variant (operational taxonomic unit 49 [OTU49]) in Chinese infants that has sequence similarities to both B. longum subsp.
29703739	19	68	theme	microcosm	2407:2415	arg1	studies					2417:2423	observational and microcosm studies	2389:2423	studies	2417:2423	With respect to infants, rapid methodologies, such as quantitative PCR, are needed to determine the prevalences and proportions of different bifidobacterial species in observational and microcosm studies in order to obtain a better understanding of the dynamics of bifidobacterial nutrition and syntrophy, knowledge that might be used to manipulate the microbiota and perhaps ensure the better health of infants.
29703739	17	69	theme	infants	2023:2029	arg1	gut					2010:2012	the gut	2006:2012	the gut of human infants	2006:2029	infantis, that dependable quantitative PCR (qPCR) differentiation between the B. longum subspecies cannot be achieved by targeting tuf gene sequences, and that functional genes involved in carbohydrate metabolism might be better targets because they delineate ecological functions.IMPORTANCE High-throughput DNA sequencing methods and advanced bioinformatics analysis have revealed the composition and biochemical capacities of microbial communities (microbiota and microbiome), including those that inhabit the gut of human infants.
29703739	17	70	theme	carbohydrate	1687:1698	arg1	metabolism					1700:1709	carbohydrate metabolism	1687:1709	carbohydrate metabolism	1687:1709	infantis, that dependable quantitative PCR (qPCR) differentiation between the B. longum subspecies cannot be achieved by targeting tuf gene sequences, and that functional genes involved in carbohydrate metabolism might be better targets because they delineate ecological functions.IMPORTANCE High-throughput DNA sequencing methods and advanced bioinformatics analysis have revealed the composition and biochemical capacities of microbial communities (microbiota and microbiome), including those that inhabit the gut of human infants.
29703739	9	71	theme	subsp	971:975	arg1	abundances					947:956	the abundances	943:956	the abundances of B. longum subsp	943:975	We evaluated the tuf gene sequence as a molecular target for quantitative PCR measurements of the abundances of B. longum subsp.
29703739	18	72	theme	interactions	2196:2207	arg1	dynamics					2169:2176	the dynamics	2165:2176	the dynamics of gut microbiota interactions	2165:2207	However, the microbiology and function of natural ecosystems have received little attention in recent decades, so an appreciation of the dynamics of gut microbiota interactions is lacking.
29703739	19	73	theme	observational	2389:2401	arg1	studies					2417:2423	observational and microcosm studies	2389:2423	studies	2417:2423	With respect to infants, rapid methodologies, such as quantitative PCR, are needed to determine the prevalences and proportions of different bifidobacterial species in observational and microcosm studies in order to obtain a better understanding of the dynamics of bifidobacterial nutrition and syntrophy, knowledge that might be used to manipulate the microbiota and perhaps ensure the better health of infants.
29703739	5	74	theme	infantis	324:331	arg1	strains					333:339	infantis strains	324:339	infantis strains that are known to colonize the infant bowel	324:383	infantis strains that are known to colonize the infant bowel are not usually differentiated in microbiota investigations.
29703739	19	75	from	prevalences	2321:2331	arg1	studies					2417:2423	observational and microcosm studies	2389:2423	studies	2417:2423	With respect to infants, rapid methodologies, such as quantitative PCR, are needed to determine the prevalences and proportions of different bifidobacterial species in observational and microcosm studies in order to obtain a better understanding of the dynamics of bifidobacterial nutrition and syntrophy, knowledge that might be used to manipulate the microbiota and perhaps ensure the better health of infants.
29703739	3	76	dep	species	226:232	arg1	longum					250:255	Bifidobacterium longum	234:255	The species Bifidobacterium longum	222:255	The species Bifidobacterium longum is prevalent, but the B. longum subsp.
29703739	18	77	dep	microbiology	2045:2056	arg1	the					2041:2043	the	2041:2043	the	2041:2043	However, the microbiology and function of natural ecosystems have received little attention in recent decades, so an appreciation of the dynamics of gut microbiota interactions is lacking.
29703739	17	78	theme	quantitative	1524:1535	arg1	qPCR					1542:1545	qPCR	1542:1545	qPCR	1542:1545	infantis, that dependable quantitative PCR (qPCR) differentiation between the B. longum subspecies cannot be achieved by targeting tuf gene sequences, and that functional genes involved in carbohydrate metabolism might be better targets because they delineate ecological functions.IMPORTANCE High-throughput DNA sequencing methods and advanced bioinformatics analysis have revealed the composition and biochemical capacities of microbial communities (microbiota and microbiome), including those that inhabit the gut of human infants.
29703739	17	78	theme	quantitative	1524:1535	arg1	PCR					1537:1539	quantitative PCR	1524:1539	dependable quantitative PCR (qPCR) differentiation between the B. longum subspecies	1513:1595	infantis, that dependable quantitative PCR (qPCR) differentiation between the B. longum subspecies cannot be achieved by targeting tuf gene sequences, and that functional genes involved in carbohydrate metabolism might be better targets because they delineate ecological functions.IMPORTANCE High-throughput DNA sequencing methods and advanced bioinformatics analysis have revealed the composition and biochemical capacities of microbial communities (microbiota and microbiome), including those that inhabit the gut of human infants.
29703739	19	79	from	proportions	2337:2347	arg1	studies					2417:2423	observational and microcosm studies	2389:2423	studies	2417:2423	With respect to infants, rapid methodologies, such as quantitative PCR, are needed to determine the prevalences and proportions of different bifidobacterial species in observational and microcosm studies in order to obtain a better understanding of the dynamics of bifidobacterial nutrition and syntrophy, knowledge that might be used to manipulate the microbiota and perhaps ensure the better health of infants.
29703739	14	80	theme	other	1385:1389	arg1	subsp					1401:1405	other B. longum subsp	1385:1405	other B. longum subsp	1385:1405	longum We compared the genome sequence and growth and transcriptional characteristics of an OTU49 isolate cultured in HMO medium to those of other B. longum subsp.
29703739	7	81	theme	species	713:719	arg1	abundances					692:701	the abundances	688:701	the abundances of target species in microbiotas and microcosms	688:749	Quantitative PCR provides a quick analytical method by which to accurately ascertain the abundances of target species in microbiotas and microcosms.
29703739	12	82	dep	resulted	1051:1058	arg1	unit					1122:1125	operational taxonomic unit 49	1100:1128	operational taxonomic unit 49 [OTU49]	1100:1136	This approach resulted in the detection of a tuf gene variant (operational taxonomic unit 49 [OTU49]) in Chinese infants that has sequence similarities to both B. longum subsp.
29703739	17	83	theme	dependable	1513:1522	arg1	differentiation					1548:1562	dependable quantitative PCR (qPCR) differentiation	1513:1562	dependable quantitative PCR (qPCR) differentiation between the B. longum subspecies	1513:1595	infantis, that dependable quantitative PCR (qPCR) differentiation between the B. longum subspecies cannot be achieved by targeting tuf gene sequences, and that functional genes involved in carbohydrate metabolism might be better targets because they delineate ecological functions.IMPORTANCE High-throughput DNA sequencing methods and advanced bioinformatics analysis have revealed the composition and biochemical capacities of microbial communities (microbiota and microbiome), including those that inhabit the gut of human infants.
29703739	11	84	theme	fecal	1018:1022	arg1	microbiotas					1024:1034	fecal microbiotas	1018:1034	fecal microbiotas	1018:1034	longum in fecal microbiotas.
29703739	17	85	dep	infantis	1498:1505	arg1	targets					1727:1733	better targets	1720:1733	better targets	1720:1733	infantis, that dependable quantitative PCR (qPCR) differentiation between the B. longum subspecies cannot be achieved by targeting tuf gene sequences, and that functional genes involved in carbohydrate metabolism might be better targets because they delineate ecological functions.IMPORTANCE High-throughput DNA sequencing methods and advanced bioinformatics analysis have revealed the composition and biochemical capacities of microbial communities (microbiota and microbiome), including those that inhabit the gut of human infants.
29703739	17	85	dep	infantis	1498:1505	arg1	achieved					1607:1614	achieved	1607:1614	cannot be achieved by targeting tuf gene sequences	1597:1646	infantis, that dependable quantitative PCR (qPCR) differentiation between the B. longum subspecies cannot be achieved by targeting tuf gene sequences, and that functional genes involved in carbohydrate metabolism might be better targets because they delineate ecological functions.IMPORTANCE High-throughput DNA sequencing methods and advanced bioinformatics analysis have revealed the composition and biochemical capacities of microbial communities (microbiota and microbiome), including those that inhabit the gut of human infants.
29703739	5	86	theme	infant	372:377	arg1	bowel					379:383	the infant bowel	368:383	the infant bowel	368:383	infantis strains that are known to colonize the infant bowel are not usually differentiated in microbiota investigations.
29703739	12	87	theme	sequence	1167:1174	arg1	similarities					1176:1187	sequence similarities	1167:1187	sequence similarities to both B. longum subsp	1167:1211	This approach resulted in the detection of a tuf gene variant (operational taxonomic unit 49 [OTU49]) in Chinese infants that has sequence similarities to both B. longum subsp.
29703739	17	88	theme	gene	1633:1636	arg1	sequences					1638:1646	tuf gene sequences	1629:1646	tuf gene sequences	1629:1646	infantis, that dependable quantitative PCR (qPCR) differentiation between the B. longum subspecies cannot be achieved by targeting tuf gene sequences, and that functional genes involved in carbohydrate metabolism might be better targets because they delineate ecological functions.IMPORTANCE High-throughput DNA sequencing methods and advanced bioinformatics analysis have revealed the composition and biochemical capacities of microbial communities (microbiota and microbiome), including those that inhabit the gut of human infants.
29703739	0	89	from	Variation	18:26	arg1	subsp					54:58	Bifidobacterium longum subsp	31:58	Bifidobacterium longum subsp	31:58	tuf Gene Sequence Variation in Bifidobacterium longum subsp.
29703739	18	90	theme	gut	2181:2183	arg1	interactions					2196:2207	gut microbiota interactions	2181:2207	gut microbiota interactions	2181:2207	However, the microbiology and function of natural ecosystems have received little attention in recent decades, so an appreciation of the dynamics of gut microbiota interactions is lacking.
29703739	18	91	theme	dynamics	2169:2176	arg1	appreciation					2149:2160	an appreciation	2146:2160	an appreciation of the dynamics of gut microbiota interactions	2146:2207	However, the microbiology and function of natural ecosystems have received little attention in recent decades, so an appreciation of the dynamics of gut microbiota interactions is lacking.
29703739	14	92	theme	HMO	1362:1364	arg1	medium					1366:1371	HMO medium	1362:1371	HMO medium	1362:1371	longum We compared the genome sequence and growth and transcriptional characteristics of an OTU49 isolate cultured in HMO medium to those of other B. longum subsp.
29703739	12	93	theme	Chinese	1142:1148	arg1	infants					1150:1156	Chinese infants	1142:1156	Chinese infants that has sequence similarities to both B. longum subsp	1142:1211	This approach resulted in the detection of a tuf gene variant (operational taxonomic unit 49 [OTU49]) in Chinese infants that has sequence similarities to both B. longum subsp.
29703739	7	94	theme	quick	631:635	arg1	method					648:653	a quick analytical method	629:653	a quick analytical method by which to accurately ascertain the abundances of target species in microbiotas and microcosms	629:749	Quantitative PCR provides a quick analytical method by which to accurately ascertain the abundances of target species in microbiotas and microcosms.
29703739	17	95	theme	biochemical	1900:1910	arg1	capacities					1912:1921	biochemical capacities	1900:1921	biochemical capacities	1900:1921	infantis, that dependable quantitative PCR (qPCR) differentiation between the B. longum subspecies cannot be achieved by targeting tuf gene sequences, and that functional genes involved in carbohydrate metabolism might be better targets because they delineate ecological functions.IMPORTANCE High-throughput DNA sequencing methods and advanced bioinformatics analysis have revealed the composition and biochemical capacities of microbial communities (microbiota and microbiome), including those that inhabit the gut of human infants.
29703739	19	96	theme	quantitative	2275:2286	arg1	PCR					2288:2290	quantitative PCR	2275:2290	quantitative PCR	2275:2290	With respect to infants, rapid methodologies, such as quantitative PCR, are needed to determine the prevalences and proportions of different bifidobacterial species in observational and microcosm studies in order to obtain a better understanding of the dynamics of bifidobacterial nutrition and syntrophy, knowledge that might be used to manipulate the microbiota and perhaps ensure the better health of infants.
29703739	14	97	theme	OTU49	1336:1340	arg1	isolate					1342:1348	an OTU49 isolate	1333:1348	an OTU49 isolate cultured in HMO medium to those of other B. longum subsp	1333:1405	longum We compared the genome sequence and growth and transcriptional characteristics of an OTU49 isolate cultured in HMO medium to those of other B. longum subsp.
29703739	0	98	theme	Gene	4:7	arg1	Variation					18:26	tuf Gene Sequence Variation	0:26	tuf Gene Sequence Variation in Bifidobacterium longum subsp	0:58	tuf Gene Sequence Variation in Bifidobacterium longum subsp.
29703739	19	99	theme	infants	2625:2631	arg1	health					2615:2620	the better health	2604:2620	the better health of infants	2604:2631	With respect to infants, rapid methodologies, such as quantitative PCR, are needed to determine the prevalences and proportions of different bifidobacterial species in observational and microcosm studies in order to obtain a better understanding of the dynamics of bifidobacterial nutrition and syntrophy, knowledge that might be used to manipulate the microbiota and perhaps ensure the better health of infants.
29703739	6	100	theme	different	551:559	arg1	roles					586:590	different ecological and symbiotic roles	551:590	different ecological and symbiotic roles	551:590	These subspecies differ in their capacities to metabolize human milk oligosaccharides (HMO) and may have different ecological and symbiotic roles in humans.
29703739	17	101	dep	communities	1936:1946	arg1	microbiota					1949:1958	microbiota	1949:1958	microbiota	1949:1958	infantis, that dependable quantitative PCR (qPCR) differentiation between the B. longum subspecies cannot be achieved by targeting tuf gene sequences, and that functional genes involved in carbohydrate metabolism might be better targets because they delineate ecological functions.IMPORTANCE High-throughput DNA sequencing methods and advanced bioinformatics analysis have revealed the composition and biochemical capacities of microbial communities (microbiota and microbiome), including those that inhabit the gut of human infants.
29703739	17	101	dep	communities	1936:1946	arg1	communities					1936:1946	microbial communities	1926:1946	microbial communities (microbiota and microbiome)	1926:1974	infantis, that dependable quantitative PCR (qPCR) differentiation between the B. longum subspecies cannot be achieved by targeting tuf gene sequences, and that functional genes involved in carbohydrate metabolism might be better targets because they delineate ecological functions.IMPORTANCE High-throughput DNA sequencing methods and advanced bioinformatics analysis have revealed the composition and biochemical capacities of microbial communities (microbiota and microbiome), including those that inhabit the gut of human infants.
29703739	17	101	dep	communities	1936:1946	arg1	those					1987:1991	those	1987:1991	those	1987:1991	infantis, that dependable quantitative PCR (qPCR) differentiation between the B. longum subspecies cannot be achieved by targeting tuf gene sequences, and that functional genes involved in carbohydrate metabolism might be better targets because they delineate ecological functions.IMPORTANCE High-throughput DNA sequencing methods and advanced bioinformatics analysis have revealed the composition and biochemical capacities of microbial communities (microbiota and microbiome), including those that inhabit the gut of human infants.
29703739	17	101	dep	communities	1936:1946	arg1	microbiome					1964:1973	microbiome	1964:1973	microbiome	1964:1973	infantis, that dependable quantitative PCR (qPCR) differentiation between the B. longum subspecies cannot be achieved by targeting tuf gene sequences, and that functional genes involved in carbohydrate metabolism might be better targets because they delineate ecological functions.IMPORTANCE High-throughput DNA sequencing methods and advanced bioinformatics analysis have revealed the composition and biochemical capacities of microbial communities (microbiota and microbiome), including those that inhabit the gut of human infants.
29703739	9	102	theme	tuf	866:868	arg1	target					899:904	a molecular target	887:904	a molecular target for quantitative PCR measurements of the abundances of B. longum subsp	887:975	We evaluated the tuf gene sequence as a molecular target for quantitative PCR measurements of the abundances of B. longum subsp.
29703739	9	102	theme	tuf	866:868	arg1	sequence					875:882	the tuf gene sequence	862:882	the tuf gene sequence	862:882	We evaluated the tuf gene sequence as a molecular target for quantitative PCR measurements of the abundances of B. longum subsp.
29703739	17	103	theme	DNA	1806:1808	arg1	methods					1821:1827	ecological functions.IMPORTANCE High-throughput DNA sequencing methods	1758:1827	ecological functions.IMPORTANCE High-throughput DNA sequencing methods	1758:1827	infantis, that dependable quantitative PCR (qPCR) differentiation between the B. longum subspecies cannot be achieved by targeting tuf gene sequences, and that functional genes involved in carbohydrate metabolism might be better targets because they delineate ecological functions.IMPORTANCE High-throughput DNA sequencing methods and advanced bioinformatics analysis have revealed the composition and biochemical capacities of microbial communities (microbiota and microbiome), including those that inhabit the gut of human infants.
29703739	12	104	contain	has	1163:1165	arg1	infants					1150:1156	Chinese infants	1142:1156	Chinese infants that has sequence similarities to both B. longum subsp	1142:1211	This approach resulted in the detection of a tuf gene variant (operational taxonomic unit 49 [OTU49]) in Chinese infants that has sequence similarities to both B. longum subsp.
29703739	12	104	contain	has	1163:1165	arg2	similarities					1176:1187	sequence similarities	1167:1187	sequence similarities to both B. longum subsp	1167:1211	This approach resulted in the detection of a tuf gene variant (operational taxonomic unit 49 [OTU49]) in Chinese infants that has sequence similarities to both B. longum subsp.
29703739	14	105	theme	transcriptional	1298:1312	arg1	characteristics					1314:1328	transcriptional characteristics	1298:1328	transcriptional characteristics	1298:1328	longum We compared the genome sequence and growth and transcriptional characteristics of an OTU49 isolate cultured in HMO medium to those of other B. longum subsp.
29703739	6	106	theme	human	504:508	arg1	HMO					533:535	HMO	533:535	HMO	533:535	These subspecies differ in their capacities to metabolize human milk oligosaccharides (HMO) and may have different ecological and symbiotic roles in humans.
29703739	6	106	theme	human	504:508	arg1	oligosaccharides					515:530	human milk oligosaccharides	504:530	human milk oligosaccharides (HMO)	504:536	These subspecies differ in their capacities to metabolize human milk oligosaccharides (HMO) and may have different ecological and symbiotic roles in humans.
29703739	12	107	theme	operational	1100:1110	arg1	unit					1122:1125	operational taxonomic unit 49	1100:1128	operational taxonomic unit 49 [OTU49]	1100:1136	This approach resulted in the detection of a tuf gene variant (operational taxonomic unit 49 [OTU49]) in Chinese infants that has sequence similarities to both B. longum subsp.
29703739	8	108	from	targets	775:781	arg1	DNA					786:788	DNA	786:788	DNA extracted from samples	786:811	However, amplification targets in DNA extracted from samples need to be dependably differential.
29703739	9	109	dep	B.	961:962	arg1	longum					964:969	longum	964:969	longum	964:969	We evaluated the tuf gene sequence as a molecular target for quantitative PCR measurements of the abundances of B. longum subsp.
29703739	18	110	theme	natural	2074:2080	arg1	ecosystems					2082:2091	natural ecosystems	2074:2091	natural ecosystems	2074:2091	However, the microbiology and function of natural ecosystems have received little attention in recent decades, so an appreciation of the dynamics of gut microbiota interactions is lacking.
29703739	2	111	theme	infants	213:219	arg1	microbiota					199:208	the fecal microbiota	189:208	the fecal microbiota of infants	189:219	Members of the bacterial genus Bifidobacterium generally dominate the fecal microbiota of infants.
29703739	12	112	theme	taxonomic	1112:1120	arg1	unit					1122:1125	operational taxonomic unit 49	1100:1128	operational taxonomic unit 49 [OTU49]	1100:1136	This approach resulted in the detection of a tuf gene variant (operational taxonomic unit 49 [OTU49]) in Chinese infants that has sequence similarities to both B. longum subsp.
29703739	9	113	theme	quantitative	910:921	arg1	measurements					927:938	quantitative PCR measurements	910:938	quantitative PCR measurements of the abundances of B. longum subsp	910:975	We evaluated the tuf gene sequence as a molecular target for quantitative PCR measurements of the abundances of B. longum subsp.
29703739	19	114	theme	syntrophy	2516:2524	arg1	knowledge					2527:2535	knowledge	2527:2535	knowledge that might be used to manipulate the microbiota and perhaps ensure the better health of infants	2527:2631	With respect to infants, rapid methodologies, such as quantitative PCR, are needed to determine the prevalences and proportions of different bifidobacterial species in observational and microcosm studies in order to obtain a better understanding of the dynamics of bifidobacterial nutrition and syntrophy, knowledge that might be used to manipulate the microbiota and perhaps ensure the better health of infants.
29703739	19	114	theme	syntrophy	2516:2524	arg1	dynamics					2474:2481	the dynamics	2470:2481	the dynamics of bifidobacterial nutrition and syntrophy	2470:2524	With respect to infants, rapid methodologies, such as quantitative PCR, are needed to determine the prevalences and proportions of different bifidobacterial species in observational and microcosm studies in order to obtain a better understanding of the dynamics of bifidobacterial nutrition and syntrophy, knowledge that might be used to manipulate the microbiota and perhaps ensure the better health of infants.
29703739	18	115	theme	little	2107:2112	arg1	attention					2114:2122	little attention	2107:2122	little attention	2107:2122	However, the microbiology and function of natural ecosystems have received little attention in recent decades, so an appreciation of the dynamics of gut microbiota interactions is lacking.
29703739	17	116	theme	ecological	1758:1767	arg1	methods					1821:1827	ecological functions.IMPORTANCE High-throughput DNA sequencing methods	1758:1827	ecological functions.IMPORTANCE High-throughput DNA sequencing methods	1758:1827	infantis, that dependable quantitative PCR (qPCR) differentiation between the B. longum subspecies cannot be achieved by targeting tuf gene sequences, and that functional genes involved in carbohydrate metabolism might be better targets because they delineate ecological functions.IMPORTANCE High-throughput DNA sequencing methods and advanced bioinformatics analysis have revealed the composition and biochemical capacities of microbial communities (microbiota and microbiome), including those that inhabit the gut of human infants.
29703739	12	117	theme	B.	1197:1198	arg1	subsp					1207:1211	both B. longum subsp	1192:1211	both B. longum subsp	1192:1211	This approach resulted in the detection of a tuf gene variant (operational taxonomic unit 49 [OTU49]) in Chinese infants that has sequence similarities to both B. longum subsp.
29703739	17	118	dep	composition	1884:1894	arg1	the					1880:1882	the	1880:1882	the	1880:1882	infantis, that dependable quantitative PCR (qPCR) differentiation between the B. longum subspecies cannot be achieved by targeting tuf gene sequences, and that functional genes involved in carbohydrate metabolism might be better targets because they delineate ecological functions.IMPORTANCE High-throughput DNA sequencing methods and advanced bioinformatics analysis have revealed the composition and biochemical capacities of microbial communities (microbiota and microbiome), including those that inhabit the gut of human infants.
29703739	19	119	theme	bifidobacterial	2362:2376	arg1	species					2378:2384	different bifidobacterial species	2352:2384	different bifidobacterial species	2352:2384	With respect to infants, rapid methodologies, such as quantitative PCR, are needed to determine the prevalences and proportions of different bifidobacterial species in observational and microcosm studies in order to obtain a better understanding of the dynamics of bifidobacterial nutrition and syntrophy, knowledge that might be used to manipulate the microbiota and perhaps ensure the better health of infants.
29703739	19	120	theme	nutrition	2502:2510	arg1	knowledge					2527:2535	knowledge	2527:2535	knowledge that might be used to manipulate the microbiota and perhaps ensure the better health of infants	2527:2631	With respect to infants, rapid methodologies, such as quantitative PCR, are needed to determine the prevalences and proportions of different bifidobacterial species in observational and microcosm studies in order to obtain a better understanding of the dynamics of bifidobacterial nutrition and syntrophy, knowledge that might be used to manipulate the microbiota and perhaps ensure the better health of infants.
29703739	19	120	theme	nutrition	2502:2510	arg1	dynamics					2474:2481	the dynamics	2470:2481	the dynamics of bifidobacterial nutrition and syntrophy	2470:2524	With respect to infants, rapid methodologies, such as quantitative PCR, are needed to determine the prevalences and proportions of different bifidobacterial species in observational and microcosm studies in order to obtain a better understanding of the dynamics of bifidobacterial nutrition and syntrophy, knowledge that might be used to manipulate the microbiota and perhaps ensure the better health of infants.
29561257	5	0	theme	strain	696:701	arg1	AR23208T					703:710	strain AR23208T	696:710	strain AR23208T	696:710	The predominant fatty acids (>10 %) of strain AR23208T were iso-C15 : 0, summed feature 4 (anteiso-C17 : 1 B and/or iso-C17 : 1 I) and anteiso-C15 : 0 and the primary isoprenoid quinone was menaquinone-7.
29561257	11	1	theme	strain	1541:1546	arg1	AR23208T					1548:1555	strain AR23208T	1541:1555	strain AR23208T	1541:1555	Based on the data presented in the current study, strain AR23208T is considered to represent a novel species of the genus Tumebacillus, for which the name Tumebacillus avium sp.
29561257	7	2	theme	sugar	1067:1071	arg1	ribose					1120:1125	ribose	1120:1125	ribose	1120:1125	The sugar components of the cell wall peptidoglycan were ribose and arabinose.
29561257	7	2	theme	sugar	1067:1071	arg1	components					1073:1082	The sugar components	1063:1082	The sugar components of the cell wall peptidoglycan	1063:1113	The sugar components of the cell wall peptidoglycan were ribose and arabinose.
29561257	11	3	theme	name	1641:1644	arg1	sp					1665:1666	the name Tumebacillus avium sp	1637:1666	the name Tumebacillus avium sp	1637:1666	Based on the data presented in the current study, strain AR23208T is considered to represent a novel species of the genus Tumebacillus, for which the name Tumebacillus avium sp.
29561257	9	4	theme	strain	1345:1350	arg1	sequence					1333:1340	the complete genome sequence	1313:1340	the complete genome sequence of strain AR23208T and the closest related strain, T. algifaecis THMBR28T,	1313:1415	The OrthoANI value based on the complete genome sequence of strain AR23208T and the closest related strain, T. algifaecis THMBR28T, was 80.4 %.
29561257	5	5	theme	AR23208T	703:710	arg1	acids					679:683	The predominant fatty acids	657:683	The predominant fatty acids (>10 %) of strain AR23208T	657:710	The predominant fatty acids (>10 %) of strain AR23208T were iso-C15 : 0, summed feature 4 (anteiso-C17 : 1 B and/or iso-C17 : 1 I) and anteiso-C15 : 0 and the primary isoprenoid quinone was menaquinone-7.
29561257	5	5	theme	AR23208T	703:710	arg1	%					690:690	>10 %	686:690	>10 %	686:690	The predominant fatty acids (>10 %) of strain AR23208T were iso-C15 : 0, summed feature 4 (anteiso-C17 : 1 B and/or iso-C17 : 1 I) and anteiso-C15 : 0 and the primary isoprenoid quinone was menaquinone-7.
29561257	5	5	theme	AR23208T	703:710	arg1	 0					726:727	 0	726:727	 0	726:727	The predominant fatty acids (>10 %) of strain AR23208T were iso-C15 : 0, summed feature 4 (anteiso-C17 : 1 B and/or iso-C17 : 1 I) and anteiso-C15 : 0 and the primary isoprenoid quinone was menaquinone-7.
29561257	6	6	theme	unidentified	1042:1053	arg1	lipids					1055:1060	ten unidentified lipids	1038:1060	ten unidentified lipids	1038:1060	The polar lipids were phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol, six unidentified phospholipids, an unidentified aminophospholipid and ten unidentified lipids.
29561257	11	7	theme	novel	1586:1590	arg1	species					1592:1598	a novel species	1584:1598	a novel species	1584:1598	Based on the data presented in the current study, strain AR23208T is considered to represent a novel species of the genus Tumebacillus, for which the name Tumebacillus avium sp.
29561257	11	8	dep	Tumebacillus	1646:1657	arg1	avium					1659:1663	avium	1659:1663	avium	1659:1663	Based on the data presented in the current study, strain AR23208T is considered to represent a novel species of the genus Tumebacillus, for which the name Tumebacillus avium sp.
29561257	11	9	theme	Tumebacillus	1646:1657	arg1	sp					1665:1666	the name Tumebacillus avium sp	1637:1666	the name Tumebacillus avium sp	1637:1666	Based on the data presented in the current study, strain AR23208T is considered to represent a novel species of the genus Tumebacillus, for which the name Tumebacillus avium sp.
29561257	2	10	theme	cinereous	284:292	arg1	vulture					294:300	a cinereous vulture	282:300	a cinereous vulture (Aegypius monachus)	282:320	A Gram-stain-positive, facultatively aerobic, spore-forming, oxidase-positive, catalase- and DNase-negative, rod-shaped and motile bacterial strain, AR23208T, was isolated from the gut of a cinereous vulture (Aegypius monachus), collected at Seoul Grand Park Zoo (Republic of Korea).
29561257	11	11	theme	current	1526:1532	arg1	study					1534:1538	the current study	1522:1538	the current study	1522:1538	Based on the data presented in the current study, strain AR23208T is considered to represent a novel species of the genus Tumebacillus, for which the name Tumebacillus avium sp.
29561257	1	12	attach	isolated	29:36	arg2	nov.					23:26	nov.	23:26	nov.	23:26	nov., isolated from the gut of a cinereous vulture, Aegypius monachus.
29561257	1	12	attach	isolated	29:36	arg1	gut					47:49	the gut	43:49	the gut of a cinereous vulture	43:72	nov., isolated from the gut of a cinereous vulture, Aegypius monachus.
29561257	10	13	theme	56.0 mol	1480:1487	arg1	content					1449:1455	The genomic DNA G+C content	1429:1455	The genomic DNA G+C content of strain AR23208T	1429:1474	The genomic DNA G+C content of strain AR23208T was 56.0 mol%.
29561257	10	13	theme	56.0 mol	1480:1487	arg1	%					1488:1488	56.0 mol%	1480:1488	56.0 mol%	1480:1488	The genomic DNA G+C content of strain AR23208T was 56.0 mol%.
29561257	2	14	theme	aerobic	131:137	arg1	AR23208T					243:250	AR23208T	243:250	AR23208T	243:250	A Gram-stain-positive, facultatively aerobic, spore-forming, oxidase-positive, catalase- and DNase-negative, rod-shaped and motile bacterial strain, AR23208T, was isolated from the gut of a cinereous vulture (Aegypius monachus), collected at Seoul Grand Park Zoo (Republic of Korea).
29561257	2	14	theme	aerobic	131:137	arg1	strain					235:240	A Gram-stain-positive, facultatively aerobic, spore-forming, oxidase-positive, catalase- and DNase-negative, rod-shaped and motile bacterial strain	94:240	strain	235:240	A Gram-stain-positive, facultatively aerobic, spore-forming, oxidase-positive, catalase- and DNase-negative, rod-shaped and motile bacterial strain, AR23208T, was isolated from the gut of a cinereous vulture (Aegypius monachus), collected at Seoul Grand Park Zoo (Republic of Korea).
29561257	6	15	theme	unidentified	972:983	arg1	phospholipids					985:997	six unidentified phospholipids	968:997	six unidentified phospholipids	968:997	The polar lipids were phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol, six unidentified phospholipids, an unidentified aminophospholipid and ten unidentified lipids.
29561257	8	16	theme	peptidoglycan	1175:1187	arg1	l-alanine					1194:1202	l-alanine	1194:1202	l-alanine	1194:1202	The amino acids of the cell wall peptidoglycan were l-alanine, aspartic acid, meso-diaminopimelic acid, l-glutamic acid, glycine and l-lysine.
29561257	8	16	theme	peptidoglycan	1175:1187	arg1	acids					1152:1156	The amino acids	1142:1156	The amino acids of the cell wall peptidoglycan	1142:1187	The amino acids of the cell wall peptidoglycan were l-alanine, aspartic acid, meso-diaminopimelic acid, l-glutamic acid, glycine and l-lysine.
29561257	6	17	theme	polar	866:870	arg1	phosphatidylethanolamine					884:907	phosphatidylethanolamine	884:907	phosphatidylethanolamine	884:907	The polar lipids were phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol, six unidentified phospholipids, an unidentified aminophospholipid and ten unidentified lipids.
29561257	6	17	theme	polar	866:870	arg1	lipids					872:877	The polar lipids	862:877	The polar lipids	862:877	The polar lipids were phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol, six unidentified phospholipids, an unidentified aminophospholipid and ten unidentified lipids.
29561257	5	18	dep	 0	726:727	arg1	anteiso-C17 					748:759	anteiso-C17 	748:759	anteiso-C17 : 1 B and/or iso-C17 : 1 I	748:785	The predominant fatty acids (>10 %) of strain AR23208T were iso-C15 : 0, summed feature 4 (anteiso-C17 : 1 B and/or iso-C17 : 1 I) and anteiso-C15 : 0 and the primary isoprenoid quinone was menaquinone-7.
29561257	3	19	theme	NaCl	452:455	arg1	absence					441:447	the absence	437:447	the absence of NaCl	437:455	Strain AR23208T grew optimally at 25-30 °C, at pH 7 and in the absence of NaCl.
29561257	8	20	theme	wall	1170:1173	arg1	peptidoglycan					1175:1187	the cell wall peptidoglycan	1161:1187	the cell wall peptidoglycan	1161:1187	The amino acids of the cell wall peptidoglycan were l-alanine, aspartic acid, meso-diaminopimelic acid, l-glutamic acid, glycine and l-lysine.
29561257	0	21	theme	avium	13:17	arg1	sp					19:20	Tumebacillus avium sp	0:20	Tumebacillus avium sp.	0:21	Tumebacillus avium sp.
29561257	1	22	theme	cinereous	56:64	arg1	vulture					66:72	a cinereous vulture	54:72	a cinereous vulture	54:72	nov., isolated from the gut of a cinereous vulture, Aegypius monachus.
29561257	9	23	theme	strain	1385:1390	arg1	sequence					1333:1340	the complete genome sequence	1313:1340	the complete genome sequence of strain AR23208T and the closest related strain, T. algifaecis THMBR28T,	1313:1415	The OrthoANI value based on the complete genome sequence of strain AR23208T and the closest related strain, T. algifaecis THMBR28T, was 80.4 %.
29561257	0	24	theme	Tumebacillus	0:11	arg1	sp					19:20	Tumebacillus avium sp	0:20	Tumebacillus avium sp.	0:21	Tumebacillus avium sp.
29561257	11	25	theme	genus	1607:1611	arg1	Tumebacillus					1613:1624	the genus Tumebacillus	1603:1624	the genus Tumebacillus	1603:1624	Based on the data presented in the current study, strain AR23208T is considered to represent a novel species of the genus Tumebacillus, for which the name Tumebacillus avium sp.
29561257	1	26	theme	vulture	66:72	arg1	gut					47:49	the gut	43:49	the gut of a cinereous vulture	43:72	nov., isolated from the gut of a cinereous vulture, Aegypius monachus.
29561257	7	27	theme	peptidoglycan	1101:1113	arg1	ribose					1120:1125	ribose	1120:1125	ribose	1120:1125	The sugar components of the cell wall peptidoglycan were ribose and arabinose.
29561257	7	27	theme	peptidoglycan	1101:1113	arg1	components					1073:1082	The sugar components	1063:1082	The sugar components of the cell wall peptidoglycan	1063:1113	The sugar components of the cell wall peptidoglycan were ribose and arabinose.
29561257	5	28	theme	summed	730:735	arg1	 0					726:727	 0	726:727	 0	726:727	The predominant fatty acids (>10 %) of strain AR23208T were iso-C15 : 0, summed feature 4 (anteiso-C17 : 1 B and/or iso-C17 : 1 I) and anteiso-C15 : 0 and the primary isoprenoid quinone was menaquinone-7.
29561257	5	28	theme	summed	730:735	arg1	feature					737:743	summed feature 4	730:745	summed feature 4	730:745	The predominant fatty acids (>10 %) of strain AR23208T were iso-C15 : 0, summed feature 4 (anteiso-C17 : 1 B and/or iso-C17 : 1 I) and anteiso-C15 : 0 and the primary isoprenoid quinone was menaquinone-7.
29561257	8	29	theme	amino	1146:1150	arg1	l-alanine					1194:1202	l-alanine	1194:1202	l-alanine	1194:1202	The amino acids of the cell wall peptidoglycan were l-alanine, aspartic acid, meso-diaminopimelic acid, l-glutamic acid, glycine and l-lysine.
29561257	8	29	theme	amino	1146:1150	arg1	acids					1152:1156	The amino acids	1142:1156	The amino acids of the cell wall peptidoglycan	1142:1187	The amino acids of the cell wall peptidoglycan were l-alanine, aspartic acid, meso-diaminopimelic acid, l-glutamic acid, glycine and l-lysine.
29561257	5	30	theme	isoprenoid	824:833	arg1	menaquinone-7					847:859	menaquinone-7	847:859	menaquinone-7	847:859	The predominant fatty acids (>10 %) of strain AR23208T were iso-C15 : 0, summed feature 4 (anteiso-C17 : 1 B and/or iso-C17 : 1 I) and anteiso-C15 : 0 and the primary isoprenoid quinone was menaquinone-7.
29561257	5	30	theme	isoprenoid	824:833	arg1	quinone					835:841	the primary isoprenoid quinone	812:841	the primary isoprenoid quinone	812:841	The predominant fatty acids (>10 %) of strain AR23208T were iso-C15 : 0, summed feature 4 (anteiso-C17 : 1 B and/or iso-C17 : 1 I) and anteiso-C15 : 0 and the primary isoprenoid quinone was menaquinone-7.
29561257	4	31	theme	sequence	547:554	arg1	similarity					556:565	98.2 and 97.1 % 16S rRNA gene sequence similarity	517:565	98.2 and 97.1 % 16S rRNA gene sequence similarity	517:565	Phylogenetic analysis revealed that strain AR23208T shared 98.2 and 97.1 % 16S rRNA gene sequence similarity with Tumebacillus algifaecis THMBR28T and Tumebacilluslipolyticus NIO-S10T, respectively.
29561257	2	32	dep	vulture	294:300	arg1	monachus					312:319	Aegypius monachus	303:319	Aegypius monachus	303:319	A Gram-stain-positive, facultatively aerobic, spore-forming, oxidase-positive, catalase- and DNase-negative, rod-shaped and motile bacterial strain, AR23208T, was isolated from the gut of a cinereous vulture (Aegypius monachus), collected at Seoul Grand Park Zoo (Republic of Korea).
29561257	4	33	theme	strain	494:499	arg1	AR23208T					501:508	strain AR23208T	494:508	strain AR23208T	494:508	Phylogenetic analysis revealed that strain AR23208T shared 98.2 and 97.1 % 16S rRNA gene sequence similarity with Tumebacillus algifaecis THMBR28T and Tumebacilluslipolyticus NIO-S10T, respectively.
29561257	5	34	theme	primary	816:822	arg1	menaquinone-7					847:859	menaquinone-7	847:859	menaquinone-7	847:859	The predominant fatty acids (>10 %) of strain AR23208T were iso-C15 : 0, summed feature 4 (anteiso-C17 : 1 B and/or iso-C17 : 1 I) and anteiso-C15 : 0 and the primary isoprenoid quinone was menaquinone-7.
29561257	5	34	theme	primary	816:822	arg1	quinone					835:841	the primary isoprenoid quinone	812:841	the primary isoprenoid quinone	812:841	The predominant fatty acids (>10 %) of strain AR23208T were iso-C15 : 0, summed feature 4 (anteiso-C17 : 1 B and/or iso-C17 : 1 I) and anteiso-C15 : 0 and the primary isoprenoid quinone was menaquinone-7.
29561257	13	35	theme	type	1691:1694	arg1	AR23208T					1706:1713	AR23208T	1706:1713	AR23208T (=KCTC 33929T=JCM 32188T)	1706:1739	The type strain is AR23208T (=KCTC 33929T=JCM 32188T).
29561257	13	35	theme	type	1691:1694	arg1	strain					1696:1701	The type strain	1687:1701	The type strain	1687:1701	The type strain is AR23208T (=KCTC 33929T=JCM 32188T).
29561257	13	36	theme	=KCTC	1716:1720	arg1	AR23208T					1706:1713	AR23208T	1706:1713	AR23208T (=KCTC 33929T=JCM 32188T)	1706:1739	The type strain is AR23208T (=KCTC 33929T=JCM 32188T).
29561257	13	36	theme	=KCTC	1716:1720	arg1	32188T					1733:1738	=KCTC 33929T=JCM 32188T	1716:1738	=KCTC 33929T=JCM 32188T	1716:1738	The type strain is AR23208T (=KCTC 33929T=JCM 32188T).
29561257	4	37	theme	gene	542:545	arg1	similarity					556:565	98.2 and 97.1 % 16S rRNA gene sequence similarity	517:565	98.2 and 97.1 % 16S rRNA gene sequence similarity	517:565	Phylogenetic analysis revealed that strain AR23208T shared 98.2 and 97.1 % 16S rRNA gene sequence similarity with Tumebacillus algifaecis THMBR28T and Tumebacilluslipolyticus NIO-S10T, respectively.
29561257	7	38	theme	cell	1091:1094	arg1	peptidoglycan					1101:1113	the cell wall peptidoglycan	1087:1113	the cell wall peptidoglycan	1087:1113	The sugar components of the cell wall peptidoglycan were ribose and arabinose.
29561257	9	39	theme	OrthoANI	1289:1296	arg1	%					1426:1426	80.4 %	1421:1426	80.4 %	1421:1426	The OrthoANI value based on the complete genome sequence of strain AR23208T and the closest related strain, T. algifaecis THMBR28T, was 80.4 %.
29561257	9	39	theme	OrthoANI	1289:1296	arg1	value					1298:1302	The OrthoANI value	1285:1302	The OrthoANI value based on the complete genome sequence of strain AR23208T and the closest related strain, T. algifaecis THMBR28T,	1285:1415	The OrthoANI value based on the complete genome sequence of strain AR23208T and the closest related strain, T. algifaecis THMBR28T, was 80.4 %.
29561257	5	40	theme	predominant	661:671	arg1	acids					679:683	The predominant fatty acids	657:683	The predominant fatty acids (>10 %) of strain AR23208T	657:710	The predominant fatty acids (>10 %) of strain AR23208T were iso-C15 : 0, summed feature 4 (anteiso-C17 : 1 B and/or iso-C17 : 1 I) and anteiso-C15 : 0 and the primary isoprenoid quinone was menaquinone-7.
29561257	5	40	theme	predominant	661:671	arg1	%					690:690	>10 %	686:690	>10 %	686:690	The predominant fatty acids (>10 %) of strain AR23208T were iso-C15 : 0, summed feature 4 (anteiso-C17 : 1 B and/or iso-C17 : 1 I) and anteiso-C15 : 0 and the primary isoprenoid quinone was menaquinone-7.
29561257	5	40	theme	predominant	661:671	arg1	 0					726:727	 0	726:727	 0	726:727	The predominant fatty acids (>10 %) of strain AR23208T were iso-C15 : 0, summed feature 4 (anteiso-C17 : 1 B and/or iso-C17 : 1 I) and anteiso-C15 : 0 and the primary isoprenoid quinone was menaquinone-7.
29561257	2	41	theme	oxidase-positive	155:170	arg1	AR23208T					243:250	AR23208T	243:250	AR23208T	243:250	A Gram-stain-positive, facultatively aerobic, spore-forming, oxidase-positive, catalase- and DNase-negative, rod-shaped and motile bacterial strain, AR23208T, was isolated from the gut of a cinereous vulture (Aegypius monachus), collected at Seoul Grand Park Zoo (Republic of Korea).
29561257	2	41	theme	oxidase-positive	155:170	arg1	strain					235:240	A Gram-stain-positive, facultatively aerobic, spore-forming, oxidase-positive, catalase- and DNase-negative, rod-shaped and motile bacterial strain	94:240	strain	235:240	A Gram-stain-positive, facultatively aerobic, spore-forming, oxidase-positive, catalase- and DNase-negative, rod-shaped and motile bacterial strain, AR23208T, was isolated from the gut of a cinereous vulture (Aegypius monachus), collected at Seoul Grand Park Zoo (Republic of Korea).
29561257	2	42	theme	bacterial	225:233	arg1	AR23208T					243:250	AR23208T	243:250	AR23208T	243:250	A Gram-stain-positive, facultatively aerobic, spore-forming, oxidase-positive, catalase- and DNase-negative, rod-shaped and motile bacterial strain, AR23208T, was isolated from the gut of a cinereous vulture (Aegypius monachus), collected at Seoul Grand Park Zoo (Republic of Korea).
29561257	2	42	theme	bacterial	225:233	arg1	strain					235:240	A Gram-stain-positive, facultatively aerobic, spore-forming, oxidase-positive, catalase- and DNase-negative, rod-shaped and motile bacterial strain	94:240	strain	235:240	A Gram-stain-positive, facultatively aerobic, spore-forming, oxidase-positive, catalase- and DNase-negative, rod-shaped and motile bacterial strain, AR23208T, was isolated from the gut of a cinereous vulture (Aegypius monachus), collected at Seoul Grand Park Zoo (Republic of Korea).
29561257	13	43	theme	33929T=JCM	1722:1731	arg1	AR23208T					1706:1713	AR23208T	1706:1713	AR23208T (=KCTC 33929T=JCM 32188T)	1706:1739	The type strain is AR23208T (=KCTC 33929T=JCM 32188T).
29561257	13	43	theme	33929T=JCM	1722:1731	arg1	32188T					1733:1738	=KCTC 33929T=JCM 32188T	1716:1738	=KCTC 33929T=JCM 32188T	1716:1738	The type strain is AR23208T (=KCTC 33929T=JCM 32188T).
29561257	7	44	theme	wall	1096:1099	arg1	peptidoglycan					1101:1113	the cell wall peptidoglycan	1087:1113	the cell wall peptidoglycan	1087:1113	The sugar components of the cell wall peptidoglycan were ribose and arabinose.
29561257	5	45	theme	fatty	673:677	arg1	acids					679:683	The predominant fatty acids	657:683	The predominant fatty acids (>10 %) of strain AR23208T	657:710	The predominant fatty acids (>10 %) of strain AR23208T were iso-C15 : 0, summed feature 4 (anteiso-C17 : 1 B and/or iso-C17 : 1 I) and anteiso-C15 : 0 and the primary isoprenoid quinone was menaquinone-7.
29561257	5	45	theme	fatty	673:677	arg1	%					690:690	>10 %	686:690	>10 %	686:690	The predominant fatty acids (>10 %) of strain AR23208T were iso-C15 : 0, summed feature 4 (anteiso-C17 : 1 B and/or iso-C17 : 1 I) and anteiso-C15 : 0 and the primary isoprenoid quinone was menaquinone-7.
29561257	5	45	theme	fatty	673:677	arg1	 0					726:727	 0	726:727	 0	726:727	The predominant fatty acids (>10 %) of strain AR23208T were iso-C15 : 0, summed feature 4 (anteiso-C17 : 1 B and/or iso-C17 : 1 I) and anteiso-C15 : 0 and the primary isoprenoid quinone was menaquinone-7.
29561257	2	46	theme	motile	218:223	arg1	AR23208T					243:250	AR23208T	243:250	AR23208T	243:250	A Gram-stain-positive, facultatively aerobic, spore-forming, oxidase-positive, catalase- and DNase-negative, rod-shaped and motile bacterial strain, AR23208T, was isolated from the gut of a cinereous vulture (Aegypius monachus), collected at Seoul Grand Park Zoo (Republic of Korea).
29561257	2	46	theme	motile	218:223	arg1	strain					235:240	A Gram-stain-positive, facultatively aerobic, spore-forming, oxidase-positive, catalase- and DNase-negative, rod-shaped and motile bacterial strain	94:240	strain	235:240	A Gram-stain-positive, facultatively aerobic, spore-forming, oxidase-positive, catalase- and DNase-negative, rod-shaped and motile bacterial strain, AR23208T, was isolated from the gut of a cinereous vulture (Aegypius monachus), collected at Seoul Grand Park Zoo (Republic of Korea).
29561257	5	47	theme	 1	761:762	arg1	B					764:764	 1 B	761:764	 1 B	761:764	The predominant fatty acids (>10 %) of strain AR23208T were iso-C15 : 0, summed feature 4 (anteiso-C17 : 1 B and/or iso-C17 : 1 I) and anteiso-C15 : 0 and the primary isoprenoid quinone was menaquinone-7.
29561257	8	48	theme	cell	1165:1168	arg1	peptidoglycan					1175:1187	the cell wall peptidoglycan	1161:1187	the cell wall peptidoglycan	1161:1187	The amino acids of the cell wall peptidoglycan were l-alanine, aspartic acid, meso-diaminopimelic acid, l-glutamic acid, glycine and l-lysine.
29561257	10	49	theme	G+C	1445:1447	arg1	content					1449:1455	The genomic DNA G+C content	1429:1455	The genomic DNA G+C content of strain AR23208T	1429:1474	The genomic DNA G+C content of strain AR23208T was 56.0 mol%.
29561257	10	49	theme	G+C	1445:1447	arg1	%					1488:1488	56.0 mol%	1480:1488	56.0 mol%	1480:1488	The genomic DNA G+C content of strain AR23208T was 56.0 mol%.
29561257	2	50	theme	spore-forming	140:152	arg1	AR23208T					243:250	AR23208T	243:250	AR23208T	243:250	A Gram-stain-positive, facultatively aerobic, spore-forming, oxidase-positive, catalase- and DNase-negative, rod-shaped and motile bacterial strain, AR23208T, was isolated from the gut of a cinereous vulture (Aegypius monachus), collected at Seoul Grand Park Zoo (Republic of Korea).
29561257	2	50	theme	spore-forming	140:152	arg1	strain					235:240	A Gram-stain-positive, facultatively aerobic, spore-forming, oxidase-positive, catalase- and DNase-negative, rod-shaped and motile bacterial strain	94:240	strain	235:240	A Gram-stain-positive, facultatively aerobic, spore-forming, oxidase-positive, catalase- and DNase-negative, rod-shaped and motile bacterial strain, AR23208T, was isolated from the gut of a cinereous vulture (Aegypius monachus), collected at Seoul Grand Park Zoo (Republic of Korea).
29561257	9	51	theme	closest	1369:1375	arg1	strain					1385:1390	the closest related strain	1365:1390	the closest related strain	1365:1390	The OrthoANI value based on the complete genome sequence of strain AR23208T and the closest related strain, T. algifaecis THMBR28T, was 80.4 %.
29561257	9	51	theme	closest	1369:1375	arg1	THMBR28T					1407:1414	THMBR28T	1407:1414	THMBR28T	1407:1414	The OrthoANI value based on the complete genome sequence of strain AR23208T and the closest related strain, T. algifaecis THMBR28T, was 80.4 %.
29561257	4	52	theme	Phylogenetic	458:469	arg1	analysis					471:478	Phylogenetic analysis	458:478	Phylogenetic analysis	458:478	Phylogenetic analysis revealed that strain AR23208T shared 98.2 and 97.1 % 16S rRNA gene sequence similarity with Tumebacillus algifaecis THMBR28T and Tumebacilluslipolyticus NIO-S10T, respectively.
29561257	10	53	theme	DNA	1441:1443	arg1	content					1449:1455	The genomic DNA G+C content	1429:1455	The genomic DNA G+C content of strain AR23208T	1429:1474	The genomic DNA G+C content of strain AR23208T was 56.0 mol%.
29561257	10	53	theme	DNA	1441:1443	arg1	%					1488:1488	56.0 mol%	1480:1488	56.0 mol%	1480:1488	The genomic DNA G+C content of strain AR23208T was 56.0 mol%.
29561257	11	54	theme	Tumebacillus	1613:1624	arg1	species					1592:1598	a novel species	1584:1598	a novel species	1584:1598	Based on the data presented in the current study, strain AR23208T is considered to represent a novel species of the genus Tumebacillus, for which the name Tumebacillus avium sp.
29561257	2	55	theme	rod-shaped	203:212	arg1	AR23208T					243:250	AR23208T	243:250	AR23208T	243:250	A Gram-stain-positive, facultatively aerobic, spore-forming, oxidase-positive, catalase- and DNase-negative, rod-shaped and motile bacterial strain, AR23208T, was isolated from the gut of a cinereous vulture (Aegypius monachus), collected at Seoul Grand Park Zoo (Republic of Korea).
29561257	2	55	theme	rod-shaped	203:212	arg1	strain					235:240	A Gram-stain-positive, facultatively aerobic, spore-forming, oxidase-positive, catalase- and DNase-negative, rod-shaped and motile bacterial strain	94:240	strain	235:240	A Gram-stain-positive, facultatively aerobic, spore-forming, oxidase-positive, catalase- and DNase-negative, rod-shaped and motile bacterial strain, AR23208T, was isolated from the gut of a cinereous vulture (Aegypius monachus), collected at Seoul Grand Park Zoo (Republic of Korea).
29561257	8	56	theme	meso-diaminopimelic	1220:1238	arg1	l-alanine					1194:1202	l-alanine	1194:1202	l-alanine	1194:1202	The amino acids of the cell wall peptidoglycan were l-alanine, aspartic acid, meso-diaminopimelic acid, l-glutamic acid, glycine and l-lysine.
29561257	8	56	theme	meso-diaminopimelic	1220:1238	arg1	acid					1240:1243	meso-diaminopimelic acid	1220:1243	meso-diaminopimelic acid	1220:1243	The amino acids of the cell wall peptidoglycan were l-alanine, aspartic acid, meso-diaminopimelic acid, l-glutamic acid, glycine and l-lysine.
29561257	2	57	attach	isolated	257:264	arg2	AR23208T					243:250	AR23208T	243:250	AR23208T	243:250	A Gram-stain-positive, facultatively aerobic, spore-forming, oxidase-positive, catalase- and DNase-negative, rod-shaped and motile bacterial strain, AR23208T, was isolated from the gut of a cinereous vulture (Aegypius monachus), collected at Seoul Grand Park Zoo (Republic of Korea).
29561257	2	57	attach	isolated	257:264	arg2	strain					235:240	A Gram-stain-positive, facultatively aerobic, spore-forming, oxidase-positive, catalase- and DNase-negative, rod-shaped and motile bacterial strain	94:240	strain	235:240	A Gram-stain-positive, facultatively aerobic, spore-forming, oxidase-positive, catalase- and DNase-negative, rod-shaped and motile bacterial strain, AR23208T, was isolated from the gut of a cinereous vulture (Aegypius monachus), collected at Seoul Grand Park Zoo (Republic of Korea).
29561257	2	57	attach	isolated	257:264	arg1	gut					275:277	the gut	271:277	the gut of a cinereous vulture (Aegypius monachus), collected at Seoul Grand Park Zoo (Republic of Korea)	271:375	A Gram-stain-positive, facultatively aerobic, spore-forming, oxidase-positive, catalase- and DNase-negative, rod-shaped and motile bacterial strain, AR23208T, was isolated from the gut of a cinereous vulture (Aegypius monachus), collected at Seoul Grand Park Zoo (Republic of Korea).
29561257	10	58	theme	genomic	1433:1439	arg1	content					1449:1455	The genomic DNA G+C content	1429:1455	The genomic DNA G+C content of strain AR23208T	1429:1474	The genomic DNA G+C content of strain AR23208T was 56.0 mol%.
29561257	10	58	theme	genomic	1433:1439	arg1	%					1488:1488	56.0 mol%	1480:1488	56.0 mol%	1480:1488	The genomic DNA G+C content of strain AR23208T was 56.0 mol%.
29561257	4	59	dep	similarity	556:565	arg1	%					531:531	%	531:531	%	531:531	Phylogenetic analysis revealed that strain AR23208T shared 98.2 and 97.1 % 16S rRNA gene sequence similarity with Tumebacillus algifaecis THMBR28T and Tumebacilluslipolyticus NIO-S10T, respectively.
29561257	8	60	theme	l-glutamic	1246:1255	arg1	l-alanine					1194:1202	l-alanine	1194:1202	l-alanine	1194:1202	The amino acids of the cell wall peptidoglycan were l-alanine, aspartic acid, meso-diaminopimelic acid, l-glutamic acid, glycine and l-lysine.
29561257	8	60	theme	l-glutamic	1246:1255	arg1	acid					1257:1260	l-glutamic acid	1246:1260	l-glutamic acid	1246:1260	The amino acids of the cell wall peptidoglycan were l-alanine, aspartic acid, meso-diaminopimelic acid, l-glutamic acid, glycine and l-lysine.
29561257	9	61	theme	complete	1317:1324	arg1	sequence					1333:1340	the complete genome sequence	1313:1340	the complete genome sequence of strain AR23208T and the closest related strain, T. algifaecis THMBR28T,	1313:1415	The OrthoANI value based on the complete genome sequence of strain AR23208T and the closest related strain, T. algifaecis THMBR28T, was 80.4 %.
29561257	4	62	dep	%	531:531	arg1	98.2					517:520	98.2	517:520	98.2	517:520	Phylogenetic analysis revealed that strain AR23208T shared 98.2 and 97.1 % 16S rRNA gene sequence similarity with Tumebacillus algifaecis THMBR28T and Tumebacilluslipolyticus NIO-S10T, respectively.
29561257	4	62	dep	%	531:531	arg1	97.1 					526:530	97.1 	526:530	97.1 	526:530	Phylogenetic analysis revealed that strain AR23208T shared 98.2 and 97.1 % 16S rRNA gene sequence similarity with Tumebacillus algifaecis THMBR28T and Tumebacilluslipolyticus NIO-S10T, respectively.
29561257	2	63	theme	vulture	294:300	arg1	gut					275:277	the gut	271:277	the gut of a cinereous vulture (Aegypius monachus), collected at Seoul Grand Park Zoo (Republic of Korea)	271:375	A Gram-stain-positive, facultatively aerobic, spore-forming, oxidase-positive, catalase- and DNase-negative, rod-shaped and motile bacterial strain, AR23208T, was isolated from the gut of a cinereous vulture (Aegypius monachus), collected at Seoul Grand Park Zoo (Republic of Korea).
29561257	9	64	theme	genome	1326:1331	arg1	sequence					1333:1340	the complete genome sequence	1313:1340	the complete genome sequence of strain AR23208T and the closest related strain, T. algifaecis THMBR28T,	1313:1415	The OrthoANI value based on the complete genome sequence of strain AR23208T and the closest related strain, T. algifaecis THMBR28T, was 80.4 %.
29561257	10	65	theme	AR23208T	1467:1474	arg1	content					1449:1455	The genomic DNA G+C content	1429:1455	The genomic DNA G+C content of strain AR23208T	1429:1474	The genomic DNA G+C content of strain AR23208T was 56.0 mol%.
29561257	10	65	theme	AR23208T	1467:1474	arg1	%					1488:1488	56.0 mol%	1480:1488	56.0 mol%	1480:1488	The genomic DNA G+C content of strain AR23208T was 56.0 mol%.
29561257	2	66	theme	catalase-	173:181	arg1	AR23208T					243:250	AR23208T	243:250	AR23208T	243:250	A Gram-stain-positive, facultatively aerobic, spore-forming, oxidase-positive, catalase- and DNase-negative, rod-shaped and motile bacterial strain, AR23208T, was isolated from the gut of a cinereous vulture (Aegypius monachus), collected at Seoul Grand Park Zoo (Republic of Korea).
29561257	2	66	theme	catalase-	173:181	arg1	strain					235:240	A Gram-stain-positive, facultatively aerobic, spore-forming, oxidase-positive, catalase- and DNase-negative, rod-shaped and motile bacterial strain	94:240	strain	235:240	A Gram-stain-positive, facultatively aerobic, spore-forming, oxidase-positive, catalase- and DNase-negative, rod-shaped and motile bacterial strain, AR23208T, was isolated from the gut of a cinereous vulture (Aegypius monachus), collected at Seoul Grand Park Zoo (Republic of Korea).
29561257	2	67	dep	Zoo	353:355	arg1	Republic					358:365	Republic	358:365	Republic	358:365	A Gram-stain-positive, facultatively aerobic, spore-forming, oxidase-positive, catalase- and DNase-negative, rod-shaped and motile bacterial strain, AR23208T, was isolated from the gut of a cinereous vulture (Aegypius monachus), collected at Seoul Grand Park Zoo (Republic of Korea).
29561257	8	68	theme	aspartic	1205:1212	arg1	l-alanine					1194:1202	l-alanine	1194:1202	l-alanine	1194:1202	The amino acids of the cell wall peptidoglycan were l-alanine, aspartic acid, meso-diaminopimelic acid, l-glutamic acid, glycine and l-lysine.
29561257	8	68	theme	aspartic	1205:1212	arg1	acid					1214:1217	aspartic acid	1205:1217	aspartic acid	1205:1217	The amino acids of the cell wall peptidoglycan were l-alanine, aspartic acid, meso-diaminopimelic acid, l-glutamic acid, glycine and l-lysine.
29561257	5	69	theme	 1	782:783	arg1	I					785:785	 1 I	782:785	anteiso-C17 : 1 B and/or iso-C17 : 1 I	748:785	The predominant fatty acids (>10 %) of strain AR23208T were iso-C15 : 0, summed feature 4 (anteiso-C17 : 1 B and/or iso-C17 : 1 I) and anteiso-C15 : 0 and the primary isoprenoid quinone was menaquinone-7.
29561257	9	70	theme	related	1377:1383	arg1	strain					1385:1390	the closest related strain	1365:1390	the closest related strain	1365:1390	The OrthoANI value based on the complete genome sequence of strain AR23208T and the closest related strain, T. algifaecis THMBR28T, was 80.4 %.
29561257	9	70	theme	related	1377:1383	arg1	THMBR28T					1407:1414	THMBR28T	1407:1414	THMBR28T	1407:1414	The OrthoANI value based on the complete genome sequence of strain AR23208T and the closest related strain, T. algifaecis THMBR28T, was 80.4 %.
29561257	4	71	theme	rRNA	537:540	arg1	similarity					556:565	98.2 and 97.1 % 16S rRNA gene sequence similarity	517:565	98.2 and 97.1 % 16S rRNA gene sequence similarity	517:565	Phylogenetic analysis revealed that strain AR23208T shared 98.2 and 97.1 % 16S rRNA gene sequence similarity with Tumebacillus algifaecis THMBR28T and Tumebacilluslipolyticus NIO-S10T, respectively.
29561257	10	72	theme	strain	1460:1465	arg1	AR23208T					1467:1474	strain AR23208T	1460:1474	strain AR23208T	1460:1474	The genomic DNA G+C content of strain AR23208T was 56.0 mol%.
29561257	2	73	theme	Gram-stain-positive	96:114	arg1	AR23208T					243:250	AR23208T	243:250	AR23208T	243:250	A Gram-stain-positive, facultatively aerobic, spore-forming, oxidase-positive, catalase- and DNase-negative, rod-shaped and motile bacterial strain, AR23208T, was isolated from the gut of a cinereous vulture (Aegypius monachus), collected at Seoul Grand Park Zoo (Republic of Korea).
29561257	2	73	theme	Gram-stain-positive	96:114	arg1	strain					235:240	A Gram-stain-positive, facultatively aerobic, spore-forming, oxidase-positive, catalase- and DNase-negative, rod-shaped and motile bacterial strain	94:240	strain	235:240	A Gram-stain-positive, facultatively aerobic, spore-forming, oxidase-positive, catalase- and DNase-negative, rod-shaped and motile bacterial strain, AR23208T, was isolated from the gut of a cinereous vulture (Aegypius monachus), collected at Seoul Grand Park Zoo (Republic of Korea).
29561257	5	74	dep	anteiso-C17 	748:759	arg1	iso-C17 					773:780	iso-C17 	773:780	iso-C17 	773:780	The predominant fatty acids (>10 %) of strain AR23208T were iso-C15 : 0, summed feature 4 (anteiso-C17 : 1 B and/or iso-C17 : 1 I) and anteiso-C15 : 0 and the primary isoprenoid quinone was menaquinone-7.
29561257	5	74	dep	anteiso-C17 	748:759	arg1	I					785:785	 1 I	782:785	anteiso-C17 : 1 B and/or iso-C17 : 1 I	748:785	The predominant fatty acids (>10 %) of strain AR23208T were iso-C15 : 0, summed feature 4 (anteiso-C17 : 1 B and/or iso-C17 : 1 I) and anteiso-C15 : 0 and the primary isoprenoid quinone was menaquinone-7.
29561257	5	74	dep	anteiso-C17 	748:759	arg1	B					764:764	 1 B	761:764	 1 B	761:764	The predominant fatty acids (>10 %) of strain AR23208T were iso-C15 : 0, summed feature 4 (anteiso-C17 : 1 B and/or iso-C17 : 1 I) and anteiso-C15 : 0 and the primary isoprenoid quinone was menaquinone-7.
29561257	1	75	dep	Aegypius	75:82	arg1	monachus					84:91	Aegypius monachus	75:91	Aegypius monachus	75:91	nov., isolated from the gut of a cinereous vulture, Aegypius monachus.
29561257	1	75	dep	Aegypius	75:82	arg1	nov.					23:26	nov.	23:26	nov.	23:26	nov., isolated from the gut of a cinereous vulture, Aegypius monachus.
29561257	4	76	theme	16S	533:535	arg1	similarity					556:565	98.2 and 97.1 % 16S rRNA gene sequence similarity	517:565	98.2 and 97.1 % 16S rRNA gene sequence similarity	517:565	Phylogenetic analysis revealed that strain AR23208T shared 98.2 and 97.1 % 16S rRNA gene sequence similarity with Tumebacillus algifaecis THMBR28T and Tumebacilluslipolyticus NIO-S10T, respectively.
30882294	12	0	theme	polar	1033:1037	arg1	lipids					1039:1044	The polar lipids	1029:1044	The polar lipids	1029:1044	The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unidentified aminophospholipid and two unidentified phospholipids.
30882294	12	0	theme	polar	1033:1037	arg1	diphosphatidylglycerol					1051:1072	diphosphatidylglycerol	1051:1072	diphosphatidylglycerol	1051:1072	The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unidentified aminophospholipid and two unidentified phospholipids.
30882294	16	1	theme	Staphylospora	1675:1687	arg1	sp					1696:1697	the type species Staphylospora marina sp	1658:1697	the type species Staphylospora marina sp	1658:1697	On the basis of polyphasic data, strain SCSIO 07575T represented a novel species of a new genus within the family Thermoactinomycetaceae, for which the name Staphylospora gen. nov. is proposed with the type species Staphylospora marina sp.
30882294	2	2	theme	sediment	246:253	arg1	sample					255:260	a deep-sea hydrothermal sediment sample	222:260	a deep-sea hydrothermal sediment sample collected from the western Pacific Ocean	222:301	A novel bacterium, designated SCSIO 07575T, was isolated from a deep-sea hydrothermal sediment sample collected from the western Pacific Ocean.
30882294	7	3	theme	substrate	629:637	arg1	mycelia					639:645	Abundant substrate mycelia	620:645	Abundant substrate mycelia	620:645	Abundant substrate mycelia were observed with grape-like spores.
30882294	2	4	theme	deep-sea	224:231	arg1	sample					255:260	a deep-sea hydrothermal sediment sample	222:260	a deep-sea hydrothermal sediment sample collected from the western Pacific Ocean	222:301	A novel bacterium, designated SCSIO 07575T, was isolated from a deep-sea hydrothermal sediment sample collected from the western Pacific Ocean.
30882294	1	5	theme	Pacific	145:151	arg1	Ocean					153:157	the Pacific Ocean	141:157	the Pacific Ocean	141:157	nov., a novel member of the family Thermoactinomycetaceae, isolated from a deep-sea hydrothermal vent in the Pacific Ocean.
30882294	15	6	theme	%	1406:1406	arg1	G+C value					1385:1393	a DNA G+C value	1379:1393	a DNA G+C value	1379:1393	Based on the whole genome sequence analysis, the genome size was 2 751 094 bp with a DNA G+C value of 57.2 mol%, including one circular chromosome and one plasmid.
30882294	15	7	theme	DNA	1381:1383	arg1	G+C value					1385:1393	a DNA G+C value	1379:1393	a DNA G+C value	1379:1393	Based on the whole genome sequence analysis, the genome size was 2 751 094 bp with a DNA G+C value of 57.2 mol%, including one circular chromosome and one plasmid.
30882294	16	8	theme	Staphylospora	1617:1629	arg1	nov.					1636:1639	the name Staphylospora gen. nov.	1608:1639	the name Staphylospora gen. nov.	1608:1639	On the basis of polyphasic data, strain SCSIO 07575T represented a novel species of a new genus within the family Thermoactinomycetaceae, for which the name Staphylospora gen. nov. is proposed with the type species Staphylospora marina sp.
30882294	14	9	theme	Major	1231:1235	arg1	acids					1243:1247	Major fatty acids	1231:1247	Major fatty acids	1231:1247	Major fatty acids were iso-C15 : 0, iso-C17 : 0 and iso-C16 : 0.
30882294	16	10	theme	SCSIO	1500:1504	arg1	07575T					1506:1511	strain SCSIO 07575T	1493:1511	strain SCSIO 07575T	1493:1511	On the basis of polyphasic data, strain SCSIO 07575T represented a novel species of a new genus within the family Thermoactinomycetaceae, for which the name Staphylospora gen. nov. is proposed with the type species Staphylospora marina sp.
30882294	11	11	contain	contained	980:988	arg2	glucose					1006:1012	glucose	1006:1012	glucose	1006:1012	Whole-cell hydrolysates contained ribose, xylose, glucose and galactose.
30882294	11	11	contain	contained	980:988	arg2	xylose					998:1003	xylose	998:1003	xylose	998:1003	Whole-cell hydrolysates contained ribose, xylose, glucose and galactose.
30882294	11	11	contain	contained	980:988	arg2	ribose					990:995	ribose	990:995	ribose	990:995	Whole-cell hydrolysates contained ribose, xylose, glucose and galactose.
30882294	11	11	contain	contained	980:988	arg1	hydrolysates					967:978	Whole-cell hydrolysates	956:978	Whole-cell hydrolysates	956:978	Whole-cell hydrolysates contained ribose, xylose, glucose and galactose.
30882294	11	11	contain	contained	980:988	arg2	galactose					1018:1026	galactose	1018:1026	galactose	1018:1026	Whole-cell hydrolysates contained ribose, xylose, glucose and galactose.
30882294	4	12	theme	optimum	371:377	arg1	conditions					379:388	The optimum conditions	367:388	The optimum conditions for growth	367:399	The optimum conditions for growth were at 55-65 °C, pH 7.0 and in the presence of 2 % (w/v) NaCl.
30882294	16	13	theme	novel	1527:1531	arg1	species					1533:1539	a novel species	1525:1539	a novel species	1525:1539	On the basis of polyphasic data, strain SCSIO 07575T represented a novel species of a new genus within the family Thermoactinomycetaceae, for which the name Staphylospora gen. nov. is proposed with the type species Staphylospora marina sp.
30882294	6	14	theme	mycelia	547:553	arg1	formation					521:529	Unstable formation	512:529	Unstable formation of white aerial mycelia	512:553	Unstable formation of white aerial mycelia was observed, which disappeared after several times' subculture.
30882294	16	15	theme	genus	1550:1554	arg1	species					1533:1539	a novel species	1525:1539	a novel species	1525:1539	On the basis of polyphasic data, strain SCSIO 07575T represented a novel species of a new genus within the family Thermoactinomycetaceae, for which the name Staphylospora gen. nov. is proposed with the type species Staphylospora marina sp.
30882294	1	16	theme	hydrothermal	120:131	arg1	vent					133:136	a deep-sea hydrothermal vent	109:136	a deep-sea hydrothermal vent in the Pacific Ocean	109:157	nov., a novel member of the family Thermoactinomycetaceae, isolated from a deep-sea hydrothermal vent in the Pacific Ocean.
30882294	2	17	attach	isolated	208:215	arg1	sample					255:260	a deep-sea hydrothermal sediment sample	222:260	a deep-sea hydrothermal sediment sample collected from the western Pacific Ocean	222:301	A novel bacterium, designated SCSIO 07575T, was isolated from a deep-sea hydrothermal sediment sample collected from the western Pacific Ocean.
30882294	2	17	attach	isolated	208:215	arg2	bacterium					168:176	A novel bacterium	160:176	A novel bacterium	160:176	A novel bacterium, designated SCSIO 07575T, was isolated from a deep-sea hydrothermal sediment sample collected from the western Pacific Ocean.
30882294	6	18	theme	white	534:538	arg1	mycelia					547:553	white aerial mycelia	534:553	white aerial mycelia	534:553	Unstable formation of white aerial mycelia was observed, which disappeared after several times' subculture.
30882294	15	19	theme	circular	1423:1430	arg1	chromosome					1432:1441	one circular chromosome	1419:1441	one circular chromosome	1419:1441	Based on the whole genome sequence analysis, the genome size was 2 751 094 bp with a DNA G+C value of 57.2 mol%, including one circular chromosome and one plasmid.
30882294	1	20	from	vent	133:136	arg1	Ocean					153:157	the Pacific Ocean	141:157	the Pacific Ocean	141:157	nov., a novel member of the family Thermoactinomycetaceae, isolated from a deep-sea hydrothermal vent in the Pacific Ocean.
30882294	9	21	theme	16S	743:745	arg1	sequences					757:765	16S rRNA gene sequences	743:765	16S rRNA gene sequences	743:765	Phylogenetic analysis of 16S rRNA gene sequences showed that SCSIO 07575T belonged to the family Thermoactinomycetaceae and formed a distinct clade in the phylogenetic tree.
30882294	9	22	theme	gene	752:755	arg1	sequences					757:765	16S rRNA gene sequences	743:765	16S rRNA gene sequences	743:765	Phylogenetic analysis of 16S rRNA gene sequences showed that SCSIO 07575T belonged to the family Thermoactinomycetaceae and formed a distinct clade in the phylogenetic tree.
30882294	15	23	theme	whole	1309:1313	arg1	analysis					1331:1338	the whole genome sequence analysis	1305:1338	the whole genome sequence analysis	1305:1338	Based on the whole genome sequence analysis, the genome size was 2 751 094 bp with a DNA G+C value of 57.2 mol%, including one circular chromosome and one plasmid.
30882294	12	24	theme	unidentified	1165:1176	arg1	phospholipids					1178:1190	two unidentified phospholipids	1161:1190	two unidentified phospholipids	1161:1190	The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unidentified aminophospholipid and two unidentified phospholipids.
30882294	5	25	theme	Strain	465:470	arg1	07575T					478:483	Strain SCSIO 07575T	465:483	Strain SCSIO 07575T	465:483	Strain SCSIO 07575T showed filamentous growth.
30882294	16	26	theme	polyphasic	1476:1485	arg1	data					1487:1490	polyphasic data	1476:1490	polyphasic data	1476:1490	On the basis of polyphasic data, strain SCSIO 07575T represented a novel species of a new genus within the family Thermoactinomycetaceae, for which the name Staphylospora gen. nov. is proposed with the type species Staphylospora marina sp.
30882294	0	27	theme	Staphylospora	0:12	arg1	nov.					26:29	Staphylospora marina gen. nov.	0:29	Staphylospora marina gen. nov.	0:29	Staphylospora marina gen. nov., sp.
30882294	9	28	theme	SCSIO	779:783	arg1	07575T					785:790	SCSIO 07575T	779:790	SCSIO 07575T	779:790	Phylogenetic analysis of 16S rRNA gene sequences showed that SCSIO 07575T belonged to the family Thermoactinomycetaceae and formed a distinct clade in the phylogenetic tree.
30882294	0	29	theme	gen.	21:24	arg1	nov.					26:29	Staphylospora marina gen. nov.	0:29	Staphylospora marina gen. nov.	0:29	Staphylospora marina gen. nov., sp.
30882294	17	30	theme	106793T=CGMCC	1744:1756	arg1	1.15879T					1758:1765	=DSM 106793T=CGMCC 1.15879T	1739:1765	=DSM 106793T=CGMCC 1.15879T	1739:1765	nov. and the type strain SCSIO 07575T (=DSM 106793T=CGMCC 1.15879T).
30882294	17	30	theme	106793T=CGMCC	1744:1756	arg1	07575T					1731:1736	the type strain SCSIO 07575T	1709:1736	the type strain SCSIO 07575T (=DSM 106793T=CGMCC 1.15879T)	1709:1766	nov. and the type strain SCSIO 07575T (=DSM 106793T=CGMCC 1.15879T).
30882294	1	31	theme	Thermoactinomycetaceae	71:92	arg1	member					50:55	a novel member	42:55	a novel member of the family Thermoactinomycetaceae	42:92	nov., a novel member of the family Thermoactinomycetaceae, isolated from a deep-sea hydrothermal vent in the Pacific Ocean.
30882294	1	31	theme	Thermoactinomycetaceae	71:92	arg1	nov.					36:39	nov.	36:39	nov.	36:39	nov., a novel member of the family Thermoactinomycetaceae, isolated from a deep-sea hydrothermal vent in the Pacific Ocean.
30882294	4	32	theme	NaCl	459:462	arg1	pH					419:420	pH 7.0	419:424	pH 7.0	419:424	The optimum conditions for growth were at 55-65 °C, pH 7.0 and in the presence of 2 % (w/v) NaCl.
30882294	4	32	theme	NaCl	459:462	arg1	55-65 °C					409:416	55-65 °C	409:416	55-65 °C	409:416	The optimum conditions for growth were at 55-65 °C, pH 7.0 and in the presence of 2 % (w/v) NaCl.
30882294	4	32	theme	NaCl	459:462	arg1	presence					437:444	the presence	433:444	the presence of 2 % (w/v) NaCl	433:462	The optimum conditions for growth were at 55-65 °C, pH 7.0 and in the presence of 2 % (w/v) NaCl.
30882294	10	33	theme	cell-wall	896:904	arg1	peptidoglycan					906:918	The cell-wall peptidoglycan	892:918	The cell-wall peptidoglycan	892:918	The cell-wall peptidoglycan contained meso-diaminopimelic acid.
30882294	4	34	theme	%	451:451	arg1	NaCl					459:462	2 % (w/v) NaCl	449:462	2 % (w/v) NaCl	449:462	The optimum conditions for growth were at 55-65 °C, pH 7.0 and in the presence of 2 % (w/v) NaCl.
30882294	14	35	dep	iso-C15 	1254:1261	arg1	 0					1276:1277	 0	1276:1277	 0	1276:1277	Major fatty acids were iso-C15 : 0, iso-C17 : 0 and iso-C16 : 0.
30882294	14	35	dep	iso-C15 	1254:1261	arg1	 0					1292:1293	 0	1292:1293	 0	1292:1293	Major fatty acids were iso-C15 : 0, iso-C17 : 0 and iso-C16 : 0.
30882294	14	35	dep	iso-C15 	1254:1261	arg1	 0					1263:1264	 0	1263:1264	 0	1263:1264	Major fatty acids were iso-C15 : 0, iso-C17 : 0 and iso-C16 : 0.
30882294	14	35	dep	iso-C15 	1254:1261	arg1	iso-C16 					1283:1290	iso-C16 	1283:1290	iso-C16 	1283:1290	Major fatty acids were iso-C15 : 0, iso-C17 : 0 and iso-C16 : 0.
30882294	15	36	theme	genome	1345:1350	arg1	size					1352:1355	the genome size	1341:1355	the genome size	1341:1355	Based on the whole genome sequence analysis, the genome size was 2 751 094 bp with a DNA G+C value of 57.2 mol%, including one circular chromosome and one plasmid.
30882294	15	36	theme	genome	1345:1350	arg1	2 751 094 bp					1361:1372	2 751 094 bp	1361:1372	2 751 094 bp	1361:1372	Based on the whole genome sequence analysis, the genome size was 2 751 094 bp with a DNA G+C value of 57.2 mol%, including one circular chromosome and one plasmid.
30882294	7	37	theme	grape-like	666:675	arg1	spores					677:682	grape-like spores	666:682	grape-like spores	666:682	Abundant substrate mycelia were observed with grape-like spores.
30882294	17	38	theme	strain	1718:1723	arg1	1.15879T					1758:1765	=DSM 106793T=CGMCC 1.15879T	1739:1765	=DSM 106793T=CGMCC 1.15879T	1739:1765	nov. and the type strain SCSIO 07575T (=DSM 106793T=CGMCC 1.15879T).
30882294	17	38	theme	strain	1718:1723	arg1	07575T					1731:1736	the type strain SCSIO 07575T	1709:1736	the type strain SCSIO 07575T (=DSM 106793T=CGMCC 1.15879T)	1709:1766	nov. and the type strain SCSIO 07575T (=DSM 106793T=CGMCC 1.15879T).
30882294	16	39	theme	species	1667:1673	arg1	sp					1696:1697	the type species Staphylospora marina sp	1658:1697	the type species Staphylospora marina sp	1658:1697	On the basis of polyphasic data, strain SCSIO 07575T represented a novel species of a new genus within the family Thermoactinomycetaceae, for which the name Staphylospora gen. nov. is proposed with the type species Staphylospora marina sp.
30882294	3	40	located	observed	324:331	arg2	Growth					304:309	Growth	304:309	Growth at 65 °C	304:318	Growth at 65 °C was observed, but not at 70 °C or below 37 °C.
30882294	3	40	located	observed	324:331	arg1	70 °C					345:349	70 °C	345:349	70 °C	345:349	Growth at 65 °C was observed, but not at 70 °C or below 37 °C.
30882294	9	41	theme	distinct	851:858	arg1	clade					860:864	a distinct clade	849:864	a distinct clade	849:864	Phylogenetic analysis of 16S rRNA gene sequences showed that SCSIO 07575T belonged to the family Thermoactinomycetaceae and formed a distinct clade in the phylogenetic tree.
30882294	15	42	theme	sequence	1322:1329	arg1	analysis					1331:1338	the whole genome sequence analysis	1305:1338	the whole genome sequence analysis	1305:1338	Based on the whole genome sequence analysis, the genome size was 2 751 094 bp with a DNA G+C value of 57.2 mol%, including one circular chromosome and one plasmid.
30882294	16	43	theme	marina	1689:1694	arg1	sp					1696:1697	the type species Staphylospora marina sp	1658:1697	the type species Staphylospora marina sp	1658:1697	On the basis of polyphasic data, strain SCSIO 07575T represented a novel species of a new genus within the family Thermoactinomycetaceae, for which the name Staphylospora gen. nov. is proposed with the type species Staphylospora marina sp.
30882294	16	44	theme	new	1546:1548	arg1	genus					1550:1554	a new genus	1544:1554	a new genus within the family Thermoactinomycetaceae	1544:1595	On the basis of polyphasic data, strain SCSIO 07575T represented a novel species of a new genus within the family Thermoactinomycetaceae, for which the name Staphylospora gen. nov. is proposed with the type species Staphylospora marina sp.
30882294	7	45	theme	Abundant	620:627	arg1	mycelia					639:645	Abundant substrate mycelia	620:645	Abundant substrate mycelia	620:645	Abundant substrate mycelia were observed with grape-like spores.
30882294	16	46	dep	data	1487:1490	arg1	basis					1467:1471	basis	1467:1471	basis	1467:1471	On the basis of polyphasic data, strain SCSIO 07575T represented a novel species of a new genus within the family Thermoactinomycetaceae, for which the name Staphylospora gen. nov. is proposed with the type species Staphylospora marina sp.
30882294	16	46	dep	data	1487:1490	arg1	the					1463:1465	the	1463:1465	the	1463:1465	On the basis of polyphasic data, strain SCSIO 07575T represented a novel species of a new genus within the family Thermoactinomycetaceae, for which the name Staphylospora gen. nov. is proposed with the type species Staphylospora marina sp.
30882294	9	47	theme	phylogenetic	873:884	arg1	tree					886:889	the phylogenetic tree	869:889	the phylogenetic tree	869:889	Phylogenetic analysis of 16S rRNA gene sequences showed that SCSIO 07575T belonged to the family Thermoactinomycetaceae and formed a distinct clade in the phylogenetic tree.
30882294	2	48	theme	hydrothermal	233:244	arg1	sample					255:260	a deep-sea hydrothermal sediment sample	222:260	a deep-sea hydrothermal sediment sample collected from the western Pacific Ocean	222:301	A novel bacterium, designated SCSIO 07575T, was isolated from a deep-sea hydrothermal sediment sample collected from the western Pacific Ocean.
30882294	6	49	theme	Unstable	512:519	arg1	formation					521:529	Unstable formation	512:529	Unstable formation of white aerial mycelia	512:553	Unstable formation of white aerial mycelia was observed, which disappeared after several times' subculture.
30882294	2	50	theme	western	281:287	arg1	Ocean					297:301	the western Pacific Ocean	277:301	the western Pacific Ocean	277:301	A novel bacterium, designated SCSIO 07575T, was isolated from a deep-sea hydrothermal sediment sample collected from the western Pacific Ocean.
30882294	15	51	with	2 751 094 bp	1361:1372	arg1	G+C value					1385:1393	a DNA G+C value	1379:1393	a DNA G+C value	1379:1393	Based on the whole genome sequence analysis, the genome size was 2 751 094 bp with a DNA G+C value of 57.2 mol%, including one circular chromosome and one plasmid.
30882294	5	52	theme	filamentous	492:502	arg1	growth					504:509	filamentous growth	492:509	filamentous growth	492:509	Strain SCSIO 07575T showed filamentous growth.
30882294	10	53	contain	contained	920:928	arg1	peptidoglycan					906:918	The cell-wall peptidoglycan	892:918	The cell-wall peptidoglycan	892:918	The cell-wall peptidoglycan contained meso-diaminopimelic acid.
30882294	10	53	contain	contained	920:928	arg2	acid					950:953	meso-diaminopimelic acid	930:953	meso-diaminopimelic acid	930:953	The cell-wall peptidoglycan contained meso-diaminopimelic acid.
30882294	15	54	theme	57.2 mol	1398:1405	arg1	%					1406:1406	57.2 mol%	1398:1406	57.2 mol%	1398:1406	Based on the whole genome sequence analysis, the genome size was 2 751 094 bp with a DNA G+C value of 57.2 mol%, including one circular chromosome and one plasmid.
30882294	3	55	from	65 °C	314:318	arg1	Growth					304:309	Growth	304:309	Growth at 65 °C	304:318	Growth at 65 °C was observed, but not at 70 °C or below 37 °C.
30882294	14	56	theme	fatty	1237:1241	arg1	acids					1243:1247	Major fatty acids	1231:1247	Major fatty acids	1231:1247	Major fatty acids were iso-C15 : 0, iso-C17 : 0 and iso-C16 : 0.
30882294	16	57	theme	name	1612:1615	arg1	nov.					1636:1639	the name Staphylospora gen. nov.	1608:1639	the name Staphylospora gen. nov.	1608:1639	On the basis of polyphasic data, strain SCSIO 07575T represented a novel species of a new genus within the family Thermoactinomycetaceae, for which the name Staphylospora gen. nov. is proposed with the type species Staphylospora marina sp.
30882294	2	58	theme	SCSIO	190:194	arg1	07575T					196:201	SCSIO 07575T	190:201	SCSIO 07575T	190:201	A novel bacterium, designated SCSIO 07575T, was isolated from a deep-sea hydrothermal sediment sample collected from the western Pacific Ocean.
30882294	13	59	theme	predominant	1197:1207	arg1	MK-7					1225:1228	MK-7	1225:1228	MK-7	1225:1228	The predominant menaquinone was MK-7.
30882294	13	59	theme	predominant	1197:1207	arg1	menaquinone					1209:1219	The predominant menaquinone	1193:1219	The predominant menaquinone	1193:1219	The predominant menaquinone was MK-7.
30882294	16	60	theme	gen.	1631:1634	arg1	nov.					1636:1639	the name Staphylospora gen. nov.	1608:1639	the name Staphylospora gen. nov.	1608:1639	On the basis of polyphasic data, strain SCSIO 07575T represented a novel species of a new genus within the family Thermoactinomycetaceae, for which the name Staphylospora gen. nov. is proposed with the type species Staphylospora marina sp.
30882294	11	61	theme	Whole-cell	956:965	arg1	hydrolysates					967:978	Whole-cell hydrolysates	956:978	Whole-cell hydrolysates	956:978	Whole-cell hydrolysates contained ribose, xylose, glucose and galactose.
30882294	1	62	theme	deep-sea	111:118	arg1	vent					133:136	a deep-sea hydrothermal vent	109:136	a deep-sea hydrothermal vent in the Pacific Ocean	109:157	nov., a novel member of the family Thermoactinomycetaceae, isolated from a deep-sea hydrothermal vent in the Pacific Ocean.
30882294	9	63	theme	Phylogenetic	718:729	arg1	analysis					731:738	Phylogenetic analysis	718:738	Phylogenetic analysis of 16S rRNA gene sequences	718:765	Phylogenetic analysis of 16S rRNA gene sequences showed that SCSIO 07575T belonged to the family Thermoactinomycetaceae and formed a distinct clade in the phylogenetic tree.
30882294	6	64	theme	aerial	540:545	arg1	mycelia					547:553	white aerial mycelia	534:553	white aerial mycelia	534:553	Unstable formation of white aerial mycelia was observed, which disappeared after several times' subculture.
30882294	8	65	theme	soluble	688:694	arg1	pigment					696:702	No soluble pigment	685:702	No soluble pigment	685:702	No soluble pigment was observed.
30882294	4	66	dep	%	451:451	arg1	w/v					454:456	w/v	454:456	w/v	454:456	The optimum conditions for growth were at 55-65 °C, pH 7.0 and in the presence of 2 % (w/v) NaCl.
30882294	9	67	theme	rRNA	747:750	arg1	sequences					757:765	16S rRNA gene sequences	743:765	16S rRNA gene sequences	743:765	Phylogenetic analysis of 16S rRNA gene sequences showed that SCSIO 07575T belonged to the family Thermoactinomycetaceae and formed a distinct clade in the phylogenetic tree.
30882294	16	68	theme	family	1567:1572	arg1	Thermoactinomycetaceae					1574:1595	the family Thermoactinomycetaceae	1563:1595	the family Thermoactinomycetaceae	1563:1595	On the basis of polyphasic data, strain SCSIO 07575T represented a novel species of a new genus within the family Thermoactinomycetaceae, for which the name Staphylospora gen. nov. is proposed with the type species Staphylospora marina sp.
30882294	1	69	theme	novel	44:48	arg1	member					50:55	a novel member	42:55	a novel member of the family Thermoactinomycetaceae	42:92	nov., a novel member of the family Thermoactinomycetaceae, isolated from a deep-sea hydrothermal vent in the Pacific Ocean.
30882294	1	69	theme	novel	44:48	arg1	nov.					36:39	nov.	36:39	nov.	36:39	nov., a novel member of the family Thermoactinomycetaceae, isolated from a deep-sea hydrothermal vent in the Pacific Ocean.
30882294	9	70	theme	sequences	757:765	arg1	analysis					731:738	Phylogenetic analysis	718:738	Phylogenetic analysis of 16S rRNA gene sequences	718:765	Phylogenetic analysis of 16S rRNA gene sequences showed that SCSIO 07575T belonged to the family Thermoactinomycetaceae and formed a distinct clade in the phylogenetic tree.
30882294	15	71	theme	genome	1315:1320	arg1	analysis					1331:1338	the whole genome sequence analysis	1305:1338	the whole genome sequence analysis	1305:1338	Based on the whole genome sequence analysis, the genome size was 2 751 094 bp with a DNA G+C value of 57.2 mol%, including one circular chromosome and one plasmid.
30882294	0	72	theme	marina	14:19	arg1	nov.					26:29	Staphylospora marina gen. nov.	0:29	Staphylospora marina gen. nov.	0:29	Staphylospora marina gen. nov., sp.
30882294	5	73	theme	SCSIO	472:476	arg1	07575T					478:483	Strain SCSIO 07575T	465:483	Strain SCSIO 07575T	465:483	Strain SCSIO 07575T showed filamentous growth.
30882294	16	74	theme	strain	1493:1498	arg1	07575T					1506:1511	strain SCSIO 07575T	1493:1511	strain SCSIO 07575T	1493:1511	On the basis of polyphasic data, strain SCSIO 07575T represented a novel species of a new genus within the family Thermoactinomycetaceae, for which the name Staphylospora gen. nov. is proposed with the type species Staphylospora marina sp.
30882294	6	75	theme	several	593:599	arg1	times					601:605	several times	593:605	several times' subculture	593:617	Unstable formation of white aerial mycelia was observed, which disappeared after several times' subculture.
30882294	17	76	theme	=DSM	1739:1742	arg1	1.15879T					1758:1765	=DSM 106793T=CGMCC 1.15879T	1739:1765	=DSM 106793T=CGMCC 1.15879T	1739:1765	nov. and the type strain SCSIO 07575T (=DSM 106793T=CGMCC 1.15879T).
30882294	17	76	theme	=DSM	1739:1742	arg1	07575T					1731:1736	the type strain SCSIO 07575T	1709:1736	the type strain SCSIO 07575T (=DSM 106793T=CGMCC 1.15879T)	1709:1766	nov. and the type strain SCSIO 07575T (=DSM 106793T=CGMCC 1.15879T).
30882294	1	77	theme	family	64:69	arg1	Thermoactinomycetaceae					71:92	the family Thermoactinomycetaceae	60:92	the family Thermoactinomycetaceae	60:92	nov., a novel member of the family Thermoactinomycetaceae, isolated from a deep-sea hydrothermal vent in the Pacific Ocean.
30882294	4	78	theme	2 	449:450	arg1	%					451:451	%	451:451	%	451:451	The optimum conditions for growth were at 55-65 °C, pH 7.0 and in the presence of 2 % (w/v) NaCl.
30882294	0	79	dep	sp	32:33	arg1	nov.					26:29	Staphylospora marina gen. nov.	0:29	Staphylospora marina gen. nov.	0:29	Staphylospora marina gen. nov., sp.
30882294	9	80	theme	family	808:813	arg1	Thermoactinomycetaceae					815:836	the family Thermoactinomycetaceae	804:836	the family Thermoactinomycetaceae	804:836	Phylogenetic analysis of 16S rRNA gene sequences showed that SCSIO 07575T belonged to the family Thermoactinomycetaceae and formed a distinct clade in the phylogenetic tree.
30882294	17	81	theme	type	1713:1716	arg1	1.15879T					1758:1765	=DSM 106793T=CGMCC 1.15879T	1739:1765	=DSM 106793T=CGMCC 1.15879T	1739:1765	nov. and the type strain SCSIO 07575T (=DSM 106793T=CGMCC 1.15879T).
30882294	17	81	theme	type	1713:1716	arg1	07575T					1731:1736	the type strain SCSIO 07575T	1709:1736	the type strain SCSIO 07575T (=DSM 106793T=CGMCC 1.15879T)	1709:1766	nov. and the type strain SCSIO 07575T (=DSM 106793T=CGMCC 1.15879T).
30882294	2	82	theme	novel	162:166	arg1	bacterium					168:176	A novel bacterium	160:176	A novel bacterium	160:176	A novel bacterium, designated SCSIO 07575T, was isolated from a deep-sea hydrothermal sediment sample collected from the western Pacific Ocean.
30882294	2	83	theme	Pacific	289:295	arg1	Ocean					297:301	the western Pacific Ocean	277:301	the western Pacific Ocean	277:301	A novel bacterium, designated SCSIO 07575T, was isolated from a deep-sea hydrothermal sediment sample collected from the western Pacific Ocean.
30882294	10	84	theme	meso-diaminopimelic	930:948	arg1	acid					950:953	meso-diaminopimelic acid	930:953	meso-diaminopimelic acid	930:953	The cell-wall peptidoglycan contained meso-diaminopimelic acid.
30882294	17	85	theme	SCSIO	1725:1729	arg1	1.15879T					1758:1765	=DSM 106793T=CGMCC 1.15879T	1739:1765	=DSM 106793T=CGMCC 1.15879T	1739:1765	nov. and the type strain SCSIO 07575T (=DSM 106793T=CGMCC 1.15879T).
30882294	17	85	theme	SCSIO	1725:1729	arg1	07575T					1731:1736	the type strain SCSIO 07575T	1709:1736	the type strain SCSIO 07575T (=DSM 106793T=CGMCC 1.15879T)	1709:1766	nov. and the type strain SCSIO 07575T (=DSM 106793T=CGMCC 1.15879T).
30882294	16	86	theme	type	1662:1665	arg1	species					1667:1673	type species	1662:1673	the type species Staphylospora marina sp	1658:1697	On the basis of polyphasic data, strain SCSIO 07575T represented a novel species of a new genus within the family Thermoactinomycetaceae, for which the name Staphylospora gen. nov. is proposed with the type species Staphylospora marina sp.
30703491	6	0	theme	HNEI	837:840	arg1	effect					843:848	the HNE inhibitory (HNEI) effect	817:848	the HNE inhibitory (HNEI) effect in vitro	817:857	The study aims to compare the HNE inhibitory (HNEI) effect in vitro and the therapeutic effect on acute lung injury (ALI) in vivo between TCLL and UA.
30703491	11	1	theme	cytokine	1489:1496	arg1	production					1498:1507	inflammatory cytokine production	1476:1507	inflammatory cytokine production	1476:1507	TCLL significantly improved the inflammatory cells and inflammatory cytokine production in mice compared with the LPS group (P < 0.05).
30703491	7	2	theme	Sivelestat	1009:1018	arg1	hydrate					1027:1033	Sivelestat sodium hydrate	1009:1033	Sivelestat sodium hydrate	1009:1033	MATERIALS AND METHODS An HNEI activity bioassay was performed with Sivelestat sodium hydrate as a positive control.
30703491	9	3	theme	uptake	1267:1272	arg1	model					1274:1278	a Caco-2 cell uptake model	1253:1278	a Caco-2 cell uptake model	1253:1278	The absorption of UA in TCLL and in UA alone was determined using a Caco-2 cell uptake model and LC-MS.
30703491	6	4	theme	inhibitory	825:834	arg1	effect					843:848	the HNE inhibitory (HNEI) effect	817:848	the HNE inhibitory (HNEI) effect in vitro	817:857	The study aims to compare the HNE inhibitory (HNEI) effect in vitro and the therapeutic effect on acute lung injury (ALI) in vivo between TCLL and UA.
30703491	12	5	theme	UA	1600:1601	arg1	P < 0.05					1616:1623	P < 0.05	1616:1623	P < 0.05	1616:1623	Additionally, it performed better than the UA alone group (P < 0.05).
30703491	12	5	theme	UA	1600:1601	arg1	group					1609:1613	the UA alone group	1596:1613	the UA alone group (P < 0.05)	1596:1624	Additionally, it performed better than the UA alone group (P < 0.05).
30703491	0	6	dep	loquat	94:99	arg1	leaves					101:106	leaves	101:106	leaves	101:106	Neutrophil elastase inhibitory effects of pentacyclic triterpenoids from Eriobotrya japonica (loquat leaves).
30703491	4	7	theme	dosage	628:633	arg1	constituents					597:608	isolated constituents	588:608	isolated constituents of the equivalent dosage	588:633	Crude plant extracts may have a greater activity than isolated constituents of the equivalent dosage.
30703491	5	8	theme	enriched	753:760	arg1	PTs					762:764	enriched PTs	753:764	enriched PTs such as UA	753:775	In this study, an Eriobotrya japonica (loquat leaves) extract (triterpenoid composition of loquat leaves, TCLL) with enriched PTs such as UA was prepared.
30703491	5	8	theme	enriched	753:760	arg1	UA					774:775	UA	774:775	UA	774:775	In this study, an Eriobotrya japonica (loquat leaves) extract (triterpenoid composition of loquat leaves, TCLL) with enriched PTs such as UA was prepared.
30703491	9	9	from	absorption	1191:1200	arg1	TCLL					1211:1214	TCLL	1211:1214	TCLL	1211:1214	The absorption of UA in TCLL and in UA alone was determined using a Caco-2 cell uptake model and LC-MS.
30703491	9	9	from	absorption	1191:1200	arg1	UA					1223:1224	UA	1223:1224	UA alone	1223:1230	The absorption of UA in TCLL and in UA alone was determined using a Caco-2 cell uptake model and LC-MS.
30703491	7	10	theme	HNEI	967:970	arg1	activity					972:979	An HNEI activity	964:979	An HNEI activity	964:979	MATERIALS AND METHODS An HNEI activity bioassay was performed with Sivelestat sodium hydrate as a positive control.
30703491	16	11	theme	PTs	1917:1919	arg1	absorption					1903:1912	Improved absorption	1894:1912	Improved absorption of PTs in TCLL	1894:1927	Improved absorption of PTs in TCLL may be one explanation for these results.
30703491	5	12	theme	japonica	665:672	arg1	extract					690:696	an Eriobotrya japonica (loquat leaves) extract	651:696	an Eriobotrya japonica (loquat leaves) extract (triterpenoid composition of loquat leaves, TCLL) with enriched PTs such as UA	651:775	In this study, an Eriobotrya japonica (loquat leaves) extract (triterpenoid composition of loquat leaves, TCLL) with enriched PTs such as UA was prepared.
30703491	5	12	theme	japonica	665:672	arg1	composition					712:722	triterpenoid composition	699:722	triterpenoid composition of loquat leaves	699:739	In this study, an Eriobotrya japonica (loquat leaves) extract (triterpenoid composition of loquat leaves, TCLL) with enriched PTs such as UA was prepared.
30703491	2	13	theme	Pentacyclic	288:298	arg1	triterpenoids					300:312	THE STUDY Pentacyclic triterpenoids	278:312	THE STUDY Pentacyclic triterpenoids (PTs)	278:318	AIM OF THE STUDY Pentacyclic triterpenoids (PTs), especially ursolic acid (UA), have been found as reversibly and competitively human neutrophil elastase (HNE) inhibitors.
30703491	2	13	theme	Pentacyclic	288:298	arg1	acid					340:343	ursolic acid	332:343	especially ursolic acid (UA)	321:348	AIM OF THE STUDY Pentacyclic triterpenoids (PTs), especially ursolic acid (UA), have been found as reversibly and competitively human neutrophil elastase (HNE) inhibitors.
30703491	2	13	theme	Pentacyclic	288:298	arg1	PTs					315:317	PTs	315:317	PTs	315:317	AIM OF THE STUDY Pentacyclic triterpenoids (PTs), especially ursolic acid (UA), have been found as reversibly and competitively human neutrophil elastase (HNE) inhibitors.
30703491	8	14	theme	lung	1093:1096	arg1	model					1111:1115	A lipopolysaccharide (LPS)-induced lung inflammatory model	1058:1115	A lipopolysaccharide (LPS)-induced lung inflammatory model	1058:1115	A lipopolysaccharide (LPS)-induced lung inflammatory model was established to evaluate TCLL's therapeutic effect on ALI in vivo.
30703491	5	15	with	extract	690:696	arg1	PTs					762:764	enriched PTs	753:764	enriched PTs such as UA	753:775	In this study, an Eriobotrya japonica (loquat leaves) extract (triterpenoid composition of loquat leaves, TCLL) with enriched PTs such as UA was prepared.
30703491	5	15	with	extract	690:696	arg1	UA					774:775	UA	774:775	UA	774:775	In this study, an Eriobotrya japonica (loquat leaves) extract (triterpenoid composition of loquat leaves, TCLL) with enriched PTs such as UA was prepared.
30703491	7	16	theme	MATERIALS	942:950	arg1	control					1049:1055	a positive control	1038:1055	a positive control	1038:1055	MATERIALS AND METHODS An HNEI activity bioassay was performed with Sivelestat sodium hydrate as a positive control.
30703491	7	16	theme	MATERIALS	942:950	arg1	bioassay					981:988	MATERIALS AND METHODS An HNEI activity bioassay	942:988	MATERIALS AND METHODS An HNEI activity bioassay	942:988	MATERIALS AND METHODS An HNEI activity bioassay was performed with Sivelestat sodium hydrate as a positive control.
30703491	4	17	contain	have	559:562	arg2	activity					574:581	a greater activity	564:581	a greater activity	564:581	Crude plant extracts may have a greater activity than isolated constituents of the equivalent dosage.
30703491	4	17	contain	have	559:562	arg1	extracts					546:553	Crude plant extracts	534:553	Crude plant extracts	534:553	Crude plant extracts may have a greater activity than isolated constituents of the equivalent dosage.
30703491	0	18	from	Eriobotrya	73:82	arg1	effects					31:37	Neutrophil elastase inhibitory effects	0:37	Neutrophil elastase inhibitory effects of pentacyclic triterpenoids from Eriobotrya japonica (loquat leaves).	0:108	Neutrophil elastase inhibitory effects of pentacyclic triterpenoids from Eriobotrya japonica (loquat leaves).
30703491	13	19	from	uptake	1641:1646	arg1	TCLL					1673:1676	TCLL	1673:1676	TCLL	1673:1676	Moreover, the uptake by Caco-2 cells of UA in TCLL was higher than that in UA alone (P < 0.05).
30703491	14	20	from	effect	1762:1767	arg1	inflammation					1818:1829	LPS-induced inflammation	1806:1829	LPS-induced inflammation	1806:1829	CONCLUSION TCLL has a significant HNEI effect in vitro and a therapeutic effect on LPS-induced inflammation in a mouse model.
30703491	1	21	theme	Eriobotrya	141:150	arg1	RELEVANCE					131:139	ETHNOPHARMACOLOGICAL RELEVANCE	110:139	ETHNOPHARMACOLOGICAL RELEVANCE Eriobotrya japonica	110:159	ETHNOPHARMACOLOGICAL RELEVANCE Eriobotrya japonica, a traditional herbal medicine in China and Japan, has long been used to treat chronic bronchitis and coughs.
30703491	1	21	theme	Eriobotrya	141:150	arg1	medicine					183:190	a traditional herbal medicine	162:190	a traditional herbal medicine in China and Japan	162:209	ETHNOPHARMACOLOGICAL RELEVANCE Eriobotrya japonica, a traditional herbal medicine in China and Japan, has long been used to treat chronic bronchitis and coughs.
30703491	8	22	from	effect	1164:1169	arg1	ALI					1174:1176	ALI	1174:1176	ALI	1174:1176	A lipopolysaccharide (LPS)-induced lung inflammatory model was established to evaluate TCLL's therapeutic effect on ALI in vivo.
30703491	1	23	theme	traditional	164:174	arg1	RELEVANCE					131:139	ETHNOPHARMACOLOGICAL RELEVANCE	110:139	ETHNOPHARMACOLOGICAL RELEVANCE Eriobotrya japonica	110:159	ETHNOPHARMACOLOGICAL RELEVANCE Eriobotrya japonica, a traditional herbal medicine in China and Japan, has long been used to treat chronic bronchitis and coughs.
30703491	1	23	theme	traditional	164:174	arg1	medicine					183:190	a traditional herbal medicine	162:190	a traditional herbal medicine in China and Japan	162:209	ETHNOPHARMACOLOGICAL RELEVANCE Eriobotrya japonica, a traditional herbal medicine in China and Japan, has long been used to treat chronic bronchitis and coughs.
30703491	5	24	theme	loquat	727:732	arg1	extract					690:696	an Eriobotrya japonica (loquat leaves) extract	651:696	an Eriobotrya japonica (loquat leaves) extract (triterpenoid composition of loquat leaves, TCLL) with enriched PTs such as UA	651:775	In this study, an Eriobotrya japonica (loquat leaves) extract (triterpenoid composition of loquat leaves, TCLL) with enriched PTs such as UA was prepared.
30703491	5	24	theme	loquat	727:732	arg1	composition					712:722	triterpenoid composition	699:722	triterpenoid composition of loquat leaves	699:739	In this study, an Eriobotrya japonica (loquat leaves) extract (triterpenoid composition of loquat leaves, TCLL) with enriched PTs such as UA was prepared.
30703491	14	25	from	effect	1796:1801	arg1	inflammation					1818:1829	LPS-induced inflammation	1806:1829	LPS-induced inflammation	1806:1829	CONCLUSION TCLL has a significant HNEI effect in vitro and a therapeutic effect on LPS-induced inflammation in a mouse model.
30703491	1	26	theme	ETHNOPHARMACOLOGICAL	110:129	arg1	RELEVANCE					131:139	ETHNOPHARMACOLOGICAL RELEVANCE	110:139	ETHNOPHARMACOLOGICAL RELEVANCE Eriobotrya japonica	110:159	ETHNOPHARMACOLOGICAL RELEVANCE Eriobotrya japonica, a traditional herbal medicine in China and Japan, has long been used to treat chronic bronchitis and coughs.
30703491	1	26	theme	ETHNOPHARMACOLOGICAL	110:129	arg1	medicine					183:190	a traditional herbal medicine	162:190	a traditional herbal medicine in China and Japan	162:209	ETHNOPHARMACOLOGICAL RELEVANCE Eriobotrya japonica, a traditional herbal medicine in China and Japan, has long been used to treat chronic bronchitis and coughs.
30703491	6	27	theme	acute	889:893	arg1	injury					900:905	acute lung injury	889:905	acute lung injury (ALI)	889:911	The study aims to compare the HNE inhibitory (HNEI) effect in vitro and the therapeutic effect on acute lung injury (ALI) in vivo between TCLL and UA.
30703491	6	27	theme	acute	889:893	arg1	ALI					908:910	ALI	908:910	ALI	908:910	The study aims to compare the HNE inhibitory (HNEI) effect in vitro and the therapeutic effect on acute lung injury (ALI) in vivo between TCLL and UA.
30703491	10	28	theme	TCLL	1318:1321	arg1	values					1308:1313	The IC50 values	1299:1313	The IC50 values of TCLL and UA for the HNEI effect	1299:1348	RESULTS The IC50 values of TCLL and UA for the HNEI effect were 3.26 ± 0.56 μg/mL and 8.49 ± 0.42 μg/mL (P < 0.01), respectively.
30703491	10	28	theme	TCLL	1318:1321	arg1	3.26 ± 0.56 μg/mL					1355:1371	3.26 ± 0.56 μg/mL	1355:1371	3.26 ± 0.56 μg/mL	1355:1371	RESULTS The IC50 values of TCLL and UA for the HNEI effect were 3.26 ± 0.56 μg/mL and 8.49 ± 0.42 μg/mL (P < 0.01), respectively.
30703491	14	29	theme	therapeutic	1784:1794	arg1	effect					1796:1801	a therapeutic effect	1782:1801	a therapeutic effect on LPS-induced inflammation	1782:1829	CONCLUSION TCLL has a significant HNEI effect in vitro and a therapeutic effect on LPS-induced inflammation in a mouse model.
30703491	16	30	theme	Improved	1894:1901	arg1	absorption					1903:1912	Improved absorption	1894:1912	Improved absorption of PTs in TCLL	1894:1927	Improved absorption of PTs in TCLL may be one explanation for these results.
30703491	0	31	theme	Neutrophil	0:9	arg1	elastase					11:18	Neutrophil elastase	0:18	Neutrophil elastase inhibitory effects of pentacyclic triterpenoids from Eriobotrya japonica (loquat leaves).	0:108	Neutrophil elastase inhibitory effects of pentacyclic triterpenoids from Eriobotrya japonica (loquat leaves).
30703491	9	32	theme	UA	1205:1206	arg1	absorption					1191:1200	The absorption	1187:1200	The absorption of UA in TCLL and in UA alone	1187:1230	The absorption of UA in TCLL and in UA alone was determined using a Caco-2 cell uptake model and LC-MS.
30703491	2	33	theme	neutrophil	405:414	arg1	elastase					416:423	human neutrophil elastase	399:423	reversibly and competitively human neutrophil elastase (HNE) inhibitors	370:440	AIM OF THE STUDY Pentacyclic triterpenoids (PTs), especially ursolic acid (UA), have been found as reversibly and competitively human neutrophil elastase (HNE) inhibitors.
30703491	2	33	theme	neutrophil	405:414	arg1	HNE					426:428	HNE	426:428	HNE	426:428	AIM OF THE STUDY Pentacyclic triterpenoids (PTs), especially ursolic acid (UA), have been found as reversibly and competitively human neutrophil elastase (HNE) inhibitors.
30703491	0	34	theme	inhibitory	20:29	arg1	effects					31:37	Neutrophil elastase inhibitory effects	0:37	Neutrophil elastase inhibitory effects of pentacyclic triterpenoids from Eriobotrya japonica (loquat leaves).	0:108	Neutrophil elastase inhibitory effects of pentacyclic triterpenoids from Eriobotrya japonica (loquat leaves).
30703491	0	35	dep	Eriobotrya	73:82	arg1	japonica					84:91	Eriobotrya japonica	73:91	Eriobotrya japonica (loquat leaves)	73:107	Neutrophil elastase inhibitory effects of pentacyclic triterpenoids from Eriobotrya japonica (loquat leaves).
30703491	10	36	theme	UA	1327:1328	arg1	values					1308:1313	The IC50 values	1299:1313	The IC50 values of TCLL and UA for the HNEI effect	1299:1348	RESULTS The IC50 values of TCLL and UA for the HNEI effect were 3.26 ± 0.56 μg/mL and 8.49 ± 0.42 μg/mL (P < 0.01), respectively.
30703491	10	36	theme	UA	1327:1328	arg1	3.26 ± 0.56 μg/mL					1355:1371	3.26 ± 0.56 μg/mL	1355:1371	3.26 ± 0.56 μg/mL	1355:1371	RESULTS The IC50 values of TCLL and UA for the HNEI effect were 3.26 ± 0.56 μg/mL and 8.49 ± 0.42 μg/mL (P < 0.01), respectively.
30703491	13	37	theme	UA	1667:1668	arg1	cells					1658:1662	Caco-2 cells	1651:1662	Caco-2 cells of UA	1651:1668	Moreover, the uptake by Caco-2 cells of UA in TCLL was higher than that in UA alone (P < 0.05).
30703491	14	38	theme	HNEI	1757:1760	arg1	effect					1762:1767	a significant HNEI effect	1743:1767	a significant HNEI effect in vitro	1743:1776	CONCLUSION TCLL has a significant HNEI effect in vitro and a therapeutic effect on LPS-induced inflammation in a mouse model.
30703491	14	39	theme	mouse	1836:1840	arg1	model					1842:1846	a mouse model	1834:1846	a mouse model	1834:1846	CONCLUSION TCLL has a significant HNEI effect in vitro and a therapeutic effect on LPS-induced inflammation in a mouse model.
30703491	2	40	theme	ursolic	332:338	arg1	triterpenoids					300:312	THE STUDY Pentacyclic triterpenoids	278:312	THE STUDY Pentacyclic triterpenoids (PTs)	278:318	AIM OF THE STUDY Pentacyclic triterpenoids (PTs), especially ursolic acid (UA), have been found as reversibly and competitively human neutrophil elastase (HNE) inhibitors.
30703491	2	40	theme	ursolic	332:338	arg1	UA					346:347	UA	346:347	UA	346:347	AIM OF THE STUDY Pentacyclic triterpenoids (PTs), especially ursolic acid (UA), have been found as reversibly and competitively human neutrophil elastase (HNE) inhibitors.
30703491	2	40	theme	ursolic	332:338	arg1	acid					340:343	ursolic acid	332:343	especially ursolic acid (UA)	321:348	AIM OF THE STUDY Pentacyclic triterpenoids (PTs), especially ursolic acid (UA), have been found as reversibly and competitively human neutrophil elastase (HNE) inhibitors.
30703491	10	41	dep	RESULTS	1291:1297	arg1	RESULTS					1291:1297	RESULTS	1291:1297	RESULTS The IC50 values of TCLL and UA for the HNEI effect were 3.26 ± 0.56 μg/mL and 8.49 ± 0.42 μg/mL (P < 0.01), respectively.	1291:1419	RESULTS The IC50 values of TCLL and UA for the HNEI effect were 3.26 ± 0.56 μg/mL and 8.49 ± 0.42 μg/mL (P < 0.01), respectively.
30703491	10	41	dep	RESULTS	1291:1297	arg1	values					1308:1313	The IC50 values	1299:1313	The IC50 values of TCLL and UA for the HNEI effect	1299:1348	RESULTS The IC50 values of TCLL and UA for the HNEI effect were 3.26 ± 0.56 μg/mL and 8.49 ± 0.42 μg/mL (P < 0.01), respectively.
30703491	10	41	dep	RESULTS	1291:1297	arg1	P < 0.01					1396:1403	P < 0.01	1396:1403	P < 0.01	1396:1403	RESULTS The IC50 values of TCLL and UA for the HNEI effect were 3.26 ± 0.56 μg/mL and 8.49 ± 0.42 μg/mL (P < 0.01), respectively.
30703491	10	41	dep	RESULTS	1291:1297	arg1	8.49 ± 0.42 μg/mL					1377:1393	8.49 ± 0.42 μg/mL	1377:1393	8.49 ± 0.42 μg/mL	1377:1393	RESULTS The IC50 values of TCLL and UA for the HNEI effect were 3.26 ± 0.56 μg/mL and 8.49 ± 0.42 μg/mL (P < 0.01), respectively.
30703491	10	41	dep	RESULTS	1291:1297	arg1	3.26 ± 0.56 μg/mL					1355:1371	3.26 ± 0.56 μg/mL	1355:1371	3.26 ± 0.56 μg/mL	1355:1371	RESULTS The IC50 values of TCLL and UA for the HNEI effect were 3.26 ± 0.56 μg/mL and 8.49 ± 0.42 μg/mL (P < 0.01), respectively.
30703491	3	42	theme	PTs	503:505	arg1	solubility					464:473	limited solubility	456:473	limited solubility	456:473	However, the limited solubility and poor bioavailability of PTs hinder their clinical use.
30703491	3	42	theme	PTs	503:505	arg1	bioavailability					484:498	poor bioavailability	479:498	poor bioavailability	479:498	However, the limited solubility and poor bioavailability of PTs hinder their clinical use.
30703491	14	43	contain	has	1739:1741	arg1	TCLL					1734:1737	CONCLUSION TCLL	1723:1737	CONCLUSION TCLL	1723:1737	CONCLUSION TCLL has a significant HNEI effect in vitro and a therapeutic effect on LPS-induced inflammation in a mouse model.
30703491	14	43	contain	has	1739:1741	arg2	effect					1762:1767	a significant HNEI effect	1743:1767	a significant HNEI effect in vitro	1743:1776	CONCLUSION TCLL has a significant HNEI effect in vitro and a therapeutic effect on LPS-induced inflammation in a mouse model.
30703491	14	43	contain	has	1739:1741	arg2	effect					1796:1801	a therapeutic effect	1782:1801	a therapeutic effect on LPS-induced inflammation	1782:1829	CONCLUSION TCLL has a significant HNEI effect in vitro and a therapeutic effect on LPS-induced inflammation in a mouse model.
30703491	11	44	theme	inflammatory	1476:1487	arg1	production					1498:1507	inflammatory cytokine production	1476:1507	inflammatory cytokine production	1476:1507	TCLL significantly improved the inflammatory cells and inflammatory cytokine production in mice compared with the LPS group (P < 0.05).
30703491	3	45	theme	poor	479:482	arg1	bioavailability					484:498	poor bioavailability	479:498	poor bioavailability	479:498	However, the limited solubility and poor bioavailability of PTs hinder their clinical use.
30703491	9	46	theme	cell	1262:1265	arg1	model					1274:1278	a Caco-2 cell uptake model	1253:1278	a Caco-2 cell uptake model	1253:1278	The absorption of UA in TCLL and in UA alone was determined using a Caco-2 cell uptake model and LC-MS.
30703491	4	47	theme	plant	540:544	arg1	extracts					546:553	Crude plant extracts	534:553	Crude plant extracts	534:553	Crude plant extracts may have a greater activity than isolated constituents of the equivalent dosage.
30703491	2	48	theme	STUDY	282:286	arg1	triterpenoids					300:312	THE STUDY Pentacyclic triterpenoids	278:312	THE STUDY Pentacyclic triterpenoids (PTs)	278:318	AIM OF THE STUDY Pentacyclic triterpenoids (PTs), especially ursolic acid (UA), have been found as reversibly and competitively human neutrophil elastase (HNE) inhibitors.
30703491	2	48	theme	STUDY	282:286	arg1	acid					340:343	ursolic acid	332:343	especially ursolic acid (UA)	321:348	AIM OF THE STUDY Pentacyclic triterpenoids (PTs), especially ursolic acid (UA), have been found as reversibly and competitively human neutrophil elastase (HNE) inhibitors.
30703491	2	48	theme	STUDY	282:286	arg1	PTs					315:317	PTs	315:317	PTs	315:317	AIM OF THE STUDY Pentacyclic triterpenoids (PTs), especially ursolic acid (UA), have been found as reversibly and competitively human neutrophil elastase (HNE) inhibitors.
30703491	6	49	theme	HNE	821:823	arg1	effect					843:848	the HNE inhibitory (HNEI) effect	817:848	the HNE inhibitory (HNEI) effect in vitro	817:857	The study aims to compare the HNE inhibitory (HNEI) effect in vitro and the therapeutic effect on acute lung injury (ALI) in vivo between TCLL and UA.
30703491	13	50	dep	alone	1705:1709	arg1	P < 0.05					1712:1719	P < 0.05	1712:1719	P < 0.05	1712:1719	Moreover, the uptake by Caco-2 cells of UA in TCLL was higher than that in UA alone (P < 0.05).
30703491	11	51	theme	LPS	1535:1537	arg1	P < 0.05					1546:1553	P < 0.05	1546:1553	P < 0.05	1546:1553	TCLL significantly improved the inflammatory cells and inflammatory cytokine production in mice compared with the LPS group (P < 0.05).
30703491	11	51	theme	LPS	1535:1537	arg1	group					1539:1543	the LPS group	1531:1543	the LPS group (P < 0.05)	1531:1554	TCLL significantly improved the inflammatory cells and inflammatory cytokine production in mice compared with the LPS group (P < 0.05).
30703491	0	52	theme	triterpenoids	54:66	arg1	effects					31:37	Neutrophil elastase inhibitory effects	0:37	Neutrophil elastase inhibitory effects of pentacyclic triterpenoids from Eriobotrya japonica (loquat leaves).	0:108	Neutrophil elastase inhibitory effects of pentacyclic triterpenoids from Eriobotrya japonica (loquat leaves).
30703491	4	53	theme	equivalent	617:626	arg1	dosage					628:633	the equivalent dosage	613:633	the equivalent dosage	613:633	Crude plant extracts may have a greater activity than isolated constituents of the equivalent dosage.
30703491	5	54	theme	Eriobotrya	654:663	arg1	japonica					665:672	Eriobotrya japonica	654:672	an Eriobotrya japonica (loquat leaves) extract (triterpenoid composition of loquat leaves, TCLL) with enriched PTs such as UA	651:775	In this study, an Eriobotrya japonica (loquat leaves) extract (triterpenoid composition of loquat leaves, TCLL) with enriched PTs such as UA was prepared.
30703491	5	54	theme	Eriobotrya	654:663	arg1	loquat					675:680	loquat leaves	675:687	loquat leaves	675:687	In this study, an Eriobotrya japonica (loquat leaves) extract (triterpenoid composition of loquat leaves, TCLL) with enriched PTs such as UA was prepared.
30703491	2	55	theme	triterpenoids	300:312	arg1	AIM					271:273	AIM	271:273	AIM OF THE STUDY Pentacyclic triterpenoids (PTs), especially ursolic acid (UA),	271:349	AIM OF THE STUDY Pentacyclic triterpenoids (PTs), especially ursolic acid (UA), have been found as reversibly and competitively human neutrophil elastase (HNE) inhibitors.
30703491	2	55	theme	triterpenoids	300:312	arg1	inhibitors					431:440	reversibly and competitively human neutrophil elastase (HNE) inhibitors	370:440	reversibly and competitively human neutrophil elastase (HNE) inhibitors	370:440	AIM OF THE STUDY Pentacyclic triterpenoids (PTs), especially ursolic acid (UA), have been found as reversibly and competitively human neutrophil elastase (HNE) inhibitors.
30703491	6	56	theme	therapeutic	867:877	arg1	effect					879:884	the therapeutic effect	863:884	the therapeutic effect on acute lung injury (ALI)	863:911	The study aims to compare the HNE inhibitory (HNEI) effect in vitro and the therapeutic effect on acute lung injury (ALI) in vivo between TCLL and UA.
30703491	5	57	dep	loquat	727:732	arg1	leaves					734:739	leaves	734:739	leaves	734:739	In this study, an Eriobotrya japonica (loquat leaves) extract (triterpenoid composition of loquat leaves, TCLL) with enriched PTs such as UA was prepared.
30703491	4	58	theme	isolated	588:595	arg1	constituents					597:608	isolated constituents	588:608	isolated constituents of the equivalent dosage	588:633	Crude plant extracts may have a greater activity than isolated constituents of the equivalent dosage.
30703491	8	59	theme	-induced	1084:1091	arg1	model					1111:1115	A lipopolysaccharide (LPS)-induced lung inflammatory model	1058:1115	A lipopolysaccharide (LPS)-induced lung inflammatory model	1058:1115	A lipopolysaccharide (LPS)-induced lung inflammatory model was established to evaluate TCLL's therapeutic effect on ALI in vivo.
30703491	5	60	dep	composition	712:722	arg1	TCLL					742:745	TCLL	742:745	TCLL	742:745	In this study, an Eriobotrya japonica (loquat leaves) extract (triterpenoid composition of loquat leaves, TCLL) with enriched PTs such as UA was prepared.
30703491	8	61	theme	therapeutic	1152:1162	arg1	effect					1164:1169	TCLL's therapeutic effect	1145:1169	TCLL's therapeutic effect on ALI	1145:1176	A lipopolysaccharide (LPS)-induced lung inflammatory model was established to evaluate TCLL's therapeutic effect on ALI in vivo.
30703491	8	62	theme	inflammatory	1098:1109	arg1	model					1111:1115	A lipopolysaccharide (LPS)-induced lung inflammatory model	1058:1115	A lipopolysaccharide (LPS)-induced lung inflammatory model	1058:1115	A lipopolysaccharide (LPS)-induced lung inflammatory model was established to evaluate TCLL's therapeutic effect on ALI in vivo.
30703491	3	63	theme	clinical	520:527	arg1	use					529:531	their clinical use	514:531	their clinical use	514:531	However, the limited solubility and poor bioavailability of PTs hinder their clinical use.
30703491	5	64	theme	triterpenoid	699:710	arg1	extract					690:696	an Eriobotrya japonica (loquat leaves) extract	651:696	an Eriobotrya japonica (loquat leaves) extract (triterpenoid composition of loquat leaves, TCLL) with enriched PTs such as UA	651:775	In this study, an Eriobotrya japonica (loquat leaves) extract (triterpenoid composition of loquat leaves, TCLL) with enriched PTs such as UA was prepared.
30703491	5	64	theme	triterpenoid	699:710	arg1	composition					712:722	triterpenoid composition	699:722	triterpenoid composition of loquat leaves	699:739	In this study, an Eriobotrya japonica (loquat leaves) extract (triterpenoid composition of loquat leaves, TCLL) with enriched PTs such as UA was prepared.
30703491	5	65	dep	loquat	675:680	arg1	leaves					682:687	leaves	682:687	leaves	682:687	In this study, an Eriobotrya japonica (loquat leaves) extract (triterpenoid composition of loquat leaves, TCLL) with enriched PTs such as UA was prepared.
30703491	1	66	theme	herbal	176:181	arg1	RELEVANCE					131:139	ETHNOPHARMACOLOGICAL RELEVANCE	110:139	ETHNOPHARMACOLOGICAL RELEVANCE Eriobotrya japonica	110:159	ETHNOPHARMACOLOGICAL RELEVANCE Eriobotrya japonica, a traditional herbal medicine in China and Japan, has long been used to treat chronic bronchitis and coughs.
30703491	1	66	theme	herbal	176:181	arg1	medicine					183:190	a traditional herbal medicine	162:190	a traditional herbal medicine in China and Japan	162:209	ETHNOPHARMACOLOGICAL RELEVANCE Eriobotrya japonica, a traditional herbal medicine in China and Japan, has long been used to treat chronic bronchitis and coughs.
30703491	7	67	theme	METHODS	956:962	arg1	control					1049:1055	a positive control	1038:1055	a positive control	1038:1055	MATERIALS AND METHODS An HNEI activity bioassay was performed with Sivelestat sodium hydrate as a positive control.
30703491	7	67	theme	METHODS	956:962	arg1	bioassay					981:988	MATERIALS AND METHODS An HNEI activity bioassay	942:988	MATERIALS AND METHODS An HNEI activity bioassay	942:988	MATERIALS AND METHODS An HNEI activity bioassay was performed with Sivelestat sodium hydrate as a positive control.
30703491	16	68	from	absorption	1903:1912	arg1	TCLL					1924:1927	TCLL	1924:1927	TCLL	1924:1927	Improved absorption of PTs in TCLL may be one explanation for these results.
30703491	1	69	from	medicine	183:190	arg1	Japan					205:209	Japan	205:209	Japan	205:209	ETHNOPHARMACOLOGICAL RELEVANCE Eriobotrya japonica, a traditional herbal medicine in China and Japan, has long been used to treat chronic bronchitis and coughs.
30703491	1	69	from	medicine	183:190	arg1	China					195:199	China	195:199	China	195:199	ETHNOPHARMACOLOGICAL RELEVANCE Eriobotrya japonica, a traditional herbal medicine in China and Japan, has long been used to treat chronic bronchitis and coughs.
30703491	14	70	theme	LPS-induced	1806:1816	arg1	inflammation					1818:1829	LPS-induced inflammation	1806:1829	LPS-induced inflammation	1806:1829	CONCLUSION TCLL has a significant HNEI effect in vitro and a therapeutic effect on LPS-induced inflammation in a mouse model.
30703491	6	71	theme	lung	895:898	arg1	injury					900:905	acute lung injury	889:905	acute lung injury (ALI)	889:911	The study aims to compare the HNE inhibitory (HNEI) effect in vitro and the therapeutic effect on acute lung injury (ALI) in vivo between TCLL and UA.
30703491	6	71	theme	lung	895:898	arg1	ALI					908:910	ALI	908:910	ALI	908:910	The study aims to compare the HNE inhibitory (HNEI) effect in vitro and the therapeutic effect on acute lung injury (ALI) in vivo between TCLL and UA.
30703491	1	72	dep	Eriobotrya	141:150	arg1	japonica					152:159	japonica	152:159	japonica	152:159	ETHNOPHARMACOLOGICAL RELEVANCE Eriobotrya japonica, a traditional herbal medicine in China and Japan, has long been used to treat chronic bronchitis and coughs.
30703491	0	73	theme	elastase	11:18	arg1	effects					31:37	Neutrophil elastase inhibitory effects	0:37	Neutrophil elastase inhibitory effects of pentacyclic triterpenoids from Eriobotrya japonica (loquat leaves).	0:108	Neutrophil elastase inhibitory effects of pentacyclic triterpenoids from Eriobotrya japonica (loquat leaves).
30703491	3	74	dep	solubility	464:473	arg1	the					452:454	the	452:454	the	452:454	However, the limited solubility and poor bioavailability of PTs hinder their clinical use.
30703491	2	75	theme	elastase	416:423	arg1	AIM					271:273	AIM	271:273	AIM OF THE STUDY Pentacyclic triterpenoids (PTs), especially ursolic acid (UA),	271:349	AIM OF THE STUDY Pentacyclic triterpenoids (PTs), especially ursolic acid (UA), have been found as reversibly and competitively human neutrophil elastase (HNE) inhibitors.
30703491	2	75	theme	elastase	416:423	arg1	inhibitors					431:440	reversibly and competitively human neutrophil elastase (HNE) inhibitors	370:440	reversibly and competitively human neutrophil elastase (HNE) inhibitors	370:440	AIM OF THE STUDY Pentacyclic triterpenoids (PTs), especially ursolic acid (UA), have been found as reversibly and competitively human neutrophil elastase (HNE) inhibitors.
30703491	10	76	theme	IC50	1303:1306	arg1	values					1308:1313	The IC50 values	1299:1313	The IC50 values of TCLL and UA for the HNEI effect	1299:1348	RESULTS The IC50 values of TCLL and UA for the HNEI effect were 3.26 ± 0.56 μg/mL and 8.49 ± 0.42 μg/mL (P < 0.01), respectively.
30703491	10	76	theme	IC50	1303:1306	arg1	3.26 ± 0.56 μg/mL					1355:1371	3.26 ± 0.56 μg/mL	1355:1371	3.26 ± 0.56 μg/mL	1355:1371	RESULTS The IC50 values of TCLL and UA for the HNEI effect were 3.26 ± 0.56 μg/mL and 8.49 ± 0.42 μg/mL (P < 0.01), respectively.
30703491	13	77	theme	Caco-2	1651:1656	arg1	cells					1658:1662	Caco-2 cells	1651:1662	Caco-2 cells of UA	1651:1668	Moreover, the uptake by Caco-2 cells of UA in TCLL was higher than that in UA alone (P < 0.05).
30703491	7	78	theme	positive	1040:1047	arg1	control					1049:1055	a positive control	1038:1055	a positive control	1038:1055	MATERIALS AND METHODS An HNEI activity bioassay was performed with Sivelestat sodium hydrate as a positive control.
30703491	7	78	theme	positive	1040:1047	arg1	bioassay					981:988	MATERIALS AND METHODS An HNEI activity bioassay	942:988	MATERIALS AND METHODS An HNEI activity bioassay	942:988	MATERIALS AND METHODS An HNEI activity bioassay was performed with Sivelestat sodium hydrate as a positive control.
30703491	2	79	theme	human	399:403	arg1	elastase					416:423	human neutrophil elastase	399:423	reversibly and competitively human neutrophil elastase (HNE) inhibitors	370:440	AIM OF THE STUDY Pentacyclic triterpenoids (PTs), especially ursolic acid (UA), have been found as reversibly and competitively human neutrophil elastase (HNE) inhibitors.
30703491	2	79	theme	human	399:403	arg1	HNE					426:428	HNE	426:428	HNE	426:428	AIM OF THE STUDY Pentacyclic triterpenoids (PTs), especially ursolic acid (UA), have been found as reversibly and competitively human neutrophil elastase (HNE) inhibitors.
30703491	4	80	theme	Crude	534:538	arg1	extracts					546:553	Crude plant extracts	534:553	Crude plant extracts	534:553	Crude plant extracts may have a greater activity than isolated constituents of the equivalent dosage.
30703491	0	81	theme	pentacyclic	42:52	arg1	triterpenoids					54:66	pentacyclic triterpenoids	42:66	pentacyclic triterpenoids	42:66	Neutrophil elastase inhibitory effects of pentacyclic triterpenoids from Eriobotrya japonica (loquat leaves).
30703491	6	82	from	effect	879:884	arg1	injury					900:905	acute lung injury	889:905	acute lung injury (ALI)	889:911	The study aims to compare the HNE inhibitory (HNEI) effect in vitro and the therapeutic effect on acute lung injury (ALI) in vivo between TCLL and UA.
30703491	6	82	from	effect	879:884	arg1	ALI					908:910	ALI	908:910	ALI	908:910	The study aims to compare the HNE inhibitory (HNEI) effect in vitro and the therapeutic effect on acute lung injury (ALI) in vivo between TCLL and UA.
30703491	7	83	theme	sodium	1020:1025	arg1	hydrate					1027:1033	Sivelestat sodium hydrate	1009:1033	Sivelestat sodium hydrate	1009:1033	MATERIALS AND METHODS An HNEI activity bioassay was performed with Sivelestat sodium hydrate as a positive control.
30703491	14	84	theme	significant	1745:1755	arg1	effect					1762:1767	a significant HNEI effect	1743:1767	a significant HNEI effect in vitro	1743:1776	CONCLUSION TCLL has a significant HNEI effect in vitro and a therapeutic effect on LPS-induced inflammation in a mouse model.
30703491	6	85	from	effect	843:848	arg1	injury					900:905	acute lung injury	889:905	acute lung injury (ALI)	889:911	The study aims to compare the HNE inhibitory (HNEI) effect in vitro and the therapeutic effect on acute lung injury (ALI) in vivo between TCLL and UA.
30703491	6	85	from	effect	843:848	arg1	ALI					908:910	ALI	908:910	ALI	908:910	The study aims to compare the HNE inhibitory (HNEI) effect in vitro and the therapeutic effect on acute lung injury (ALI) in vivo between TCLL and UA.
30703491	3	86	theme	limited	456:462	arg1	solubility					464:473	limited solubility	456:473	limited solubility	456:473	However, the limited solubility and poor bioavailability of PTs hinder their clinical use.
30703491	4	87	theme	greater	566:572	arg1	activity					574:581	a greater activity	564:581	a greater activity	564:581	Crude plant extracts may have a greater activity than isolated constituents of the equivalent dosage.
30703491	10	88	theme	HNEI	1338:1341	arg1	effect					1343:1348	the HNEI effect	1334:1348	the HNEI effect	1334:1348	RESULTS The IC50 values of TCLL and UA for the HNEI effect were 3.26 ± 0.56 μg/mL and 8.49 ± 0.42 μg/mL (P < 0.01), respectively.
30703491	1	89	used	used	226:229	arg2	medicine					183:190	a traditional herbal medicine	162:190	a traditional herbal medicine in China and Japan	162:209	ETHNOPHARMACOLOGICAL RELEVANCE Eriobotrya japonica, a traditional herbal medicine in China and Japan, has long been used to treat chronic bronchitis and coughs.
30703491	1	89	used	used	226:229	arg2	RELEVANCE					131:139	ETHNOPHARMACOLOGICAL RELEVANCE	110:139	ETHNOPHARMACOLOGICAL RELEVANCE Eriobotrya japonica	110:159	ETHNOPHARMACOLOGICAL RELEVANCE Eriobotrya japonica, a traditional herbal medicine in China and Japan, has long been used to treat chronic bronchitis and coughs.
30703491	14	90	theme	CONCLUSION	1723:1732	arg1	TCLL					1734:1737	CONCLUSION TCLL	1723:1737	CONCLUSION TCLL	1723:1737	CONCLUSION TCLL has a significant HNEI effect in vitro and a therapeutic effect on LPS-induced inflammation in a mouse model.
30703491	7	91	dep	MATERIALS	942:950	arg1	activity					972:979	An HNEI activity	964:979	An HNEI activity	964:979	MATERIALS AND METHODS An HNEI activity bioassay was performed with Sivelestat sodium hydrate as a positive control.
30703491	1	92	theme	chronic	240:246	arg1	bronchitis					248:257	chronic bronchitis	240:257	chronic bronchitis	240:257	ETHNOPHARMACOLOGICAL RELEVANCE Eriobotrya japonica, a traditional herbal medicine in China and Japan, has long been used to treat chronic bronchitis and coughs.
30703491	11	93	theme	inflammatory	1453:1464	arg1	cells					1466:1470	the inflammatory cells	1449:1470	the inflammatory cells	1449:1470	TCLL significantly improved the inflammatory cells and inflammatory cytokine production in mice compared with the LPS group (P < 0.05).
30703491	9	94	theme	Caco-2	1255:1260	arg1	model					1274:1278	a Caco-2 cell uptake model	1253:1278	a Caco-2 cell uptake model	1253:1278	The absorption of UA in TCLL and in UA alone was determined using a Caco-2 cell uptake model and LC-MS.
31908045	8	0	theme	increased	1070:1078	arg1	tolerance					1080:1088	slightly increased tolerance	1061:1088	slightly increased tolerance to DSS-induced experimental colitis	1061:1124	In contrast, mice fed a Mn-supplemented diet showed slightly increased tolerance to DSS-induced experimental colitis, as judged by the colon length.
31908045	4	1	theme	Mn	459:460	arg1	contribution					443:454	the contribution	439:454	the contribution of Mn to IBD	439:467	To date, the roles of Mn in intestinal homeostasis remain unknown and the contribution of Mn to IBD has yet to be explored.
31908045	5	2	theme	barrier	577:583	arg1	maintenance					547:557	the maintenance	543:557	the maintenance of the intestinal barrier	543:583	Here, we provide evidence that Mn is critical for the maintenance of the intestinal barrier and that Mn deficiency exacerbates dextran sulfate sodium (DSS)-induced colitis in mice.
31908045	7	3	theme	DSS	891:893	arg1	treatment					895:903	DSS treatment	891:903	DSS treatment	891:903	Even without DSS treatment, dietary Mn deficiency alone increased intestinal permeability by impairing intestinal tight junctions.
31908045	3	4	theme	essential	282:290	arg1	micronutrient					292:304	an essential micronutrient	279:304	an essential micronutrient required for normal cell function and physiological processes	279:366	Mn is an essential micronutrient required for normal cell function and physiological processes.
31908045	3	4	theme	essential	282:290	arg1	Mn					273:274	Mn	273:274	Mn	273:274	Mn is an essential micronutrient required for normal cell function and physiological processes.
31908045	10	5	theme	proper	1360:1365	arg1	maintenance					1367:1377	proper maintenance	1360:1377	proper maintenance of the intestinal barrier	1360:1403	We conclude that Mn is necessary for proper maintenance of the intestinal barrier and provides protection against DSS-induced colon injury.
31908045	6	6	theme	increased	736:744	arg1	morbidity					746:754	increased morbidity	736:754	increased morbidity	736:754	Specifically, when treated with DSS, Mn-deficient mice showed increased morbidity, weight loss, and colon injury, with a concomitant increase in inflammatory cytokine levels and oxidative and DNA damage.
31908045	9	7	theme	well-appreciated	1170:1185	arg1	roles					1187:1191	the well-appreciated roles	1166:1191	the well-appreciated roles of intestinal microbiota in driving inflammation in IBD	1166:1247	Despite the well-appreciated roles of intestinal microbiota in driving inflammation in IBD, the gut microbiome composition was not altered by changes in dietary Mn.
31908045	9	8	from	changes	1300:1306	arg1	Mn					1319:1320	dietary Mn	1311:1320	dietary Mn	1311:1320	Despite the well-appreciated roles of intestinal microbiota in driving inflammation in IBD, the gut microbiome composition was not altered by changes in dietary Mn.
31908045	3	9	theme	cell	326:329	arg1	function					331:338	normal cell function	319:338	normal cell function	319:338	Mn is an essential micronutrient required for normal cell function and physiological processes.
31908045	0	10	from	Impact	0:5	arg1	colitis					44:50	experimental colitis	31:50	experimental colitis in mice	31:58	Impact of dietary manganese on experimental colitis in mice.
31908045	2	11	theme	IBD	259:261	arg1	patients					263:270	IBD patients	259:270	IBD patients	259:270	A recent epidemiological study has shown an inverse relationship between nutritional manganese (Mn) status and IBD patients.
31908045	6	12	theme	concomitant	795:805	arg1	increase					807:814	a concomitant increase	793:814	a concomitant increase in inflammatory cytokine levels	793:846	Specifically, when treated with DSS, Mn-deficient mice showed increased morbidity, weight loss, and colon injury, with a concomitant increase in inflammatory cytokine levels and oxidative and DNA damage.
31908045	5	13	theme	Mn	594:595	arg1	deficiency					597:606	Mn deficiency	594:606	Mn deficiency	594:606	Here, we provide evidence that Mn is critical for the maintenance of the intestinal barrier and that Mn deficiency exacerbates dextran sulfate sodium (DSS)-induced colitis in mice.
31908045	8	14	theme	experimental	1105:1116	arg1	colitis					1118:1124	DSS-induced experimental colitis	1093:1124	DSS-induced experimental colitis	1093:1124	In contrast, mice fed a Mn-supplemented diet showed slightly increased tolerance to DSS-induced experimental colitis, as judged by the colon length.
31908045	0	15	from	colitis	44:50	arg1	mice					55:58	mice	55:58	mice	55:58	Impact of dietary manganese on experimental colitis in mice.
31908045	10	16	theme	barrier	1397:1403	arg1	maintenance					1367:1377	proper maintenance	1360:1377	proper maintenance of the intestinal barrier	1360:1403	We conclude that Mn is necessary for proper maintenance of the intestinal barrier and provides protection against DSS-induced colon injury.
31908045	6	17	theme	Mn-deficient	711:722	arg1	mice					724:727	Mn-deficient mice	711:727	Mn-deficient mice	711:727	Specifically, when treated with DSS, Mn-deficient mice showed increased morbidity, weight loss, and colon injury, with a concomitant increase in inflammatory cytokine levels and oxidative and DNA damage.
31908045	10	18	theme	DSS-induced	1437:1447	arg1	injury					1455:1460	DSS-induced colon injury	1437:1460	DSS-induced colon injury	1437:1460	We conclude that Mn is necessary for proper maintenance of the intestinal barrier and provides protection against DSS-induced colon injury.
31908045	3	19	theme	normal	319:324	arg1	function					331:338	normal cell function	319:338	normal cell function	319:338	Mn is an essential micronutrient required for normal cell function and physiological processes.
31908045	9	20	theme	microbiota	1207:1216	arg1	roles					1187:1191	the well-appreciated roles	1166:1191	the well-appreciated roles of intestinal microbiota in driving inflammation in IBD	1166:1247	Despite the well-appreciated roles of intestinal microbiota in driving inflammation in IBD, the gut microbiome composition was not altered by changes in dietary Mn.
31908045	9	21	theme	gut	1254:1256	arg1	composition					1269:1279	the gut microbiome composition	1250:1279	the gut microbiome composition	1250:1279	Despite the well-appreciated roles of intestinal microbiota in driving inflammation in IBD, the gut microbiome composition was not altered by changes in dietary Mn.
31908045	9	22	theme	microbiome	1258:1267	arg1	composition					1269:1279	the gut microbiome composition	1250:1279	the gut microbiome composition	1250:1279	Despite the well-appreciated roles of intestinal microbiota in driving inflammation in IBD, the gut microbiome composition was not altered by changes in dietary Mn.
31908045	2	23	theme	epidemiological	157:171	arg1	study					173:177	A recent epidemiological study	148:177	A recent epidemiological study	148:177	A recent epidemiological study has shown an inverse relationship between nutritional manganese (Mn) status and IBD patients.
31908045	1	24	theme	significant	74:84	arg1	role					86:89	a significant role	72:89	a significant role	72:89	Diet plays a significant role in the pathogenesis of inflammatory bowel disease (IBD).
31908045	6	25	theme	colon	774:778	arg1	injury					780:785	colon injury	774:785	colon injury	774:785	Specifically, when treated with DSS, Mn-deficient mice showed increased morbidity, weight loss, and colon injury, with a concomitant increase in inflammatory cytokine levels and oxidative and DNA damage.
31908045	0	26	theme	manganese	18:26	arg1	Impact					0:5	Impact	0:5	Impact of dietary manganese on experimental colitis in mice.	0:59	Impact of dietary manganese on experimental colitis in mice.
31908045	6	27	from	damage	870:875	arg1	levels					841:846	inflammatory cytokine levels	819:846	inflammatory cytokine levels	819:846	Specifically, when treated with DSS, Mn-deficient mice showed increased morbidity, weight loss, and colon injury, with a concomitant increase in inflammatory cytokine levels and oxidative and DNA damage.
31908045	3	28	theme	physiological	344:356	arg1	processes					358:366	physiological processes	344:366	physiological processes	344:366	Mn is an essential micronutrient required for normal cell function and physiological processes.
31908045	2	29	theme	recent	150:155	arg1	study					173:177	A recent epidemiological study	148:177	A recent epidemiological study	148:177	A recent epidemiological study has shown an inverse relationship between nutritional manganese (Mn) status and IBD patients.
31908045	7	30	theme	intestinal	944:953	arg1	permeability					955:966	intestinal permeability	944:966	intestinal permeability	944:966	Even without DSS treatment, dietary Mn deficiency alone increased intestinal permeability by impairing intestinal tight junctions.
31908045	6	31	theme	inflammatory	819:830	arg1	levels					841:846	inflammatory cytokine levels	819:846	inflammatory cytokine levels	819:846	Specifically, when treated with DSS, Mn-deficient mice showed increased morbidity, weight loss, and colon injury, with a concomitant increase in inflammatory cytokine levels and oxidative and DNA damage.
31908045	0	32	theme	experimental	31:42	arg1	colitis					44:50	experimental colitis	31:50	experimental colitis in mice	31:58	Impact of dietary manganese on experimental colitis in mice.
31908045	6	33	theme	DNA	866:868	arg1	damage					870:875	oxidative and DNA damage	852:875	damage	870:875	Specifically, when treated with DSS, Mn-deficient mice showed increased morbidity, weight loss, and colon injury, with a concomitant increase in inflammatory cytokine levels and oxidative and DNA damage.
31908045	9	34	from	inflammation	1229:1240	arg1	IBD					1245:1247	IBD	1245:1247	IBD	1245:1247	Despite the well-appreciated roles of intestinal microbiota in driving inflammation in IBD, the gut microbiome composition was not altered by changes in dietary Mn.
31908045	4	35	theme	intestinal	397:406	arg1	homeostasis					408:418	intestinal homeostasis	397:418	intestinal homeostasis	397:418	To date, the roles of Mn in intestinal homeostasis remain unknown and the contribution of Mn to IBD has yet to be explored.
31908045	0	36	theme	dietary	10:16	arg1	manganese					18:26	dietary manganese	10:26	dietary manganese	10:26	Impact of dietary manganese on experimental colitis in mice.
31908045	7	37	theme	intestinal	981:990	arg1	junctions					998:1006	intestinal tight junctions	981:1006	intestinal tight junctions	981:1006	Even without DSS treatment, dietary Mn deficiency alone increased intestinal permeability by impairing intestinal tight junctions.
31908045	8	38	theme	DSS-induced	1093:1103	arg1	colitis					1118:1124	DSS-induced experimental colitis	1093:1124	DSS-induced experimental colitis	1093:1124	In contrast, mice fed a Mn-supplemented diet showed slightly increased tolerance to DSS-induced experimental colitis, as judged by the colon length.
31908045	5	39	theme	-induced	648:655	arg1	colitis					657:663	dextran sulfate sodium (DSS)-induced colitis	620:663	dextran sulfate sodium (DSS)-induced colitis in mice	620:671	Here, we provide evidence that Mn is critical for the maintenance of the intestinal barrier and that Mn deficiency exacerbates dextran sulfate sodium (DSS)-induced colitis in mice.
31908045	5	40	from	colitis	657:663	arg1	mice					668:671	mice	668:671	mice	668:671	Here, we provide evidence that Mn is critical for the maintenance of the intestinal barrier and that Mn deficiency exacerbates dextran sulfate sodium (DSS)-induced colitis in mice.
31908045	2	41	theme	inverse	192:198	arg1	relationship					200:211	an inverse relationship	189:211	an inverse relationship between nutritional manganese (Mn) status and IBD patients	189:270	A recent epidemiological study has shown an inverse relationship between nutritional manganese (Mn) status and IBD patients.
31908045	10	42	theme	colon	1449:1453	arg1	injury					1455:1460	DSS-induced colon injury	1437:1460	DSS-induced colon injury	1437:1460	We conclude that Mn is necessary for proper maintenance of the intestinal barrier and provides protection against DSS-induced colon injury.
31908045	8	43	theme	colon	1144:1148	arg1	length					1150:1155	the colon length	1140:1155	the colon length	1140:1155	In contrast, mice fed a Mn-supplemented diet showed slightly increased tolerance to DSS-induced experimental colitis, as judged by the colon length.
31908045	7	44	theme	tight	992:996	arg1	junctions					998:1006	intestinal tight junctions	981:1006	intestinal tight junctions	981:1006	Even without DSS treatment, dietary Mn deficiency alone increased intestinal permeability by impairing intestinal tight junctions.
31908045	10	45	theme	intestinal	1386:1395	arg1	barrier					1397:1403	the intestinal barrier	1382:1403	the intestinal barrier	1382:1403	We conclude that Mn is necessary for proper maintenance of the intestinal barrier and provides protection against DSS-induced colon injury.
31908045	7	46	theme	dietary	906:912	arg1	deficiency					917:926	dietary Mn deficiency	906:926	dietary Mn deficiency	906:926	Even without DSS treatment, dietary Mn deficiency alone increased intestinal permeability by impairing intestinal tight junctions.
31908045	5	47	theme	intestinal	566:575	arg1	barrier					577:583	the intestinal barrier	562:583	the intestinal barrier	562:583	Here, we provide evidence that Mn is critical for the maintenance of the intestinal barrier and that Mn deficiency exacerbates dextran sulfate sodium (DSS)-induced colitis in mice.
31908045	8	48	theme	Mn-supplemented	1033:1047	arg1	diet					1049:1052	a Mn-supplemented diet	1031:1052	a Mn-supplemented diet	1031:1052	In contrast, mice fed a Mn-supplemented diet showed slightly increased tolerance to DSS-induced experimental colitis, as judged by the colon length.
31908045	6	49	theme	weight	757:762	arg1	loss					764:767	weight loss	757:767	weight loss	757:767	Specifically, when treated with DSS, Mn-deficient mice showed increased morbidity, weight loss, and colon injury, with a concomitant increase in inflammatory cytokine levels and oxidative and DNA damage.
31908045	7	50	theme	Mn	914:915	arg1	deficiency					917:926	dietary Mn deficiency	906:926	dietary Mn deficiency	906:926	Even without DSS treatment, dietary Mn deficiency alone increased intestinal permeability by impairing intestinal tight junctions.
31908045	9	51	theme	dietary	1311:1317	arg1	Mn					1319:1320	dietary Mn	1311:1320	dietary Mn	1311:1320	Despite the well-appreciated roles of intestinal microbiota in driving inflammation in IBD, the gut microbiome composition was not altered by changes in dietary Mn.
31908045	4	52	from	roles	382:386	arg1	homeostasis					408:418	intestinal homeostasis	397:418	intestinal homeostasis	397:418	To date, the roles of Mn in intestinal homeostasis remain unknown and the contribution of Mn to IBD has yet to be explored.
31908045	9	53	theme	intestinal	1196:1205	arg1	microbiota					1207:1216	intestinal microbiota	1196:1216	intestinal microbiota	1196:1216	Despite the well-appreciated roles of intestinal microbiota in driving inflammation in IBD, the gut microbiome composition was not altered by changes in dietary Mn.
31908045	1	54	theme	inflammatory	114:125	arg1	disease					133:139	inflammatory bowel disease	114:139	inflammatory bowel disease (IBD)	114:145	Diet plays a significant role in the pathogenesis of inflammatory bowel disease (IBD).
31908045	1	54	theme	inflammatory	114:125	arg1	IBD					142:144	IBD	142:144	IBD	142:144	Diet plays a significant role in the pathogenesis of inflammatory bowel disease (IBD).
31908045	4	55	theme	Mn	391:392	arg1	roles					382:386	the roles	378:386	the roles of Mn in intestinal homeostasis	378:418	To date, the roles of Mn in intestinal homeostasis remain unknown and the contribution of Mn to IBD has yet to be explored.
31908045	2	56	theme	manganese	233:241	arg1	status					248:253	nutritional manganese (Mn) status	221:253	nutritional manganese (Mn) status	221:253	A recent epidemiological study has shown an inverse relationship between nutritional manganese (Mn) status and IBD patients.
31908045	1	57	theme	bowel	127:131	arg1	disease					133:139	inflammatory bowel disease	114:139	inflammatory bowel disease (IBD)	114:145	Diet plays a significant role in the pathogenesis of inflammatory bowel disease (IBD).
31908045	1	57	theme	bowel	127:131	arg1	IBD					142:144	IBD	142:144	IBD	142:144	Diet plays a significant role in the pathogenesis of inflammatory bowel disease (IBD).
31908045	6	58	from	increase	807:814	arg1	levels					841:846	inflammatory cytokine levels	819:846	inflammatory cytokine levels	819:846	Specifically, when treated with DSS, Mn-deficient mice showed increased morbidity, weight loss, and colon injury, with a concomitant increase in inflammatory cytokine levels and oxidative and DNA damage.
31908045	6	59	theme	cytokine	832:839	arg1	levels					841:846	inflammatory cytokine levels	819:846	inflammatory cytokine levels	819:846	Specifically, when treated with DSS, Mn-deficient mice showed increased morbidity, weight loss, and colon injury, with a concomitant increase in inflammatory cytokine levels and oxidative and DNA damage.
31908045	2	60	theme	nutritional	221:231	arg1	status					248:253	nutritional manganese (Mn) status	221:253	nutritional manganese (Mn) status	221:253	A recent epidemiological study has shown an inverse relationship between nutritional manganese (Mn) status and IBD patients.
31908045	1	61	theme	disease	133:139	arg1	pathogenesis					98:109	the pathogenesis	94:109	the pathogenesis of inflammatory bowel disease (IBD)	94:145	Diet plays a significant role in the pathogenesis of inflammatory bowel disease (IBD).
31908045	6	62	theme	oxidative	852:860	arg1	damage					870:875	oxidative and DNA damage	852:875	damage	870:875	Specifically, when treated with DSS, Mn-deficient mice showed increased morbidity, weight loss, and colon injury, with a concomitant increase in inflammatory cytokine levels and oxidative and DNA damage.
31349414	6	0	theme	fibre	910:914	arg1	3.2 MPa					933:939	0.8 MPa to 3.2 MPa	922:939	0.8 MPa to 3.2 MPa	922:939	From tensile tests, we found that Young's modulus increases with CS content and is higher for films (2.5 MPa to 6.5 MPa) than for the corresponding fibre mats (0.8 MPa to 3.2 MPa).
31349414	6	0	theme	fibre	910:914	arg1	mats					916:919	the corresponding fibre mats	892:919	the corresponding fibre mats (0.8 MPa to 3.2 MPa)	892:940	From tensile tests, we found that Young's modulus increases with CS content and is higher for films (2.5 MPa to 6.5 MPa) than for the corresponding fibre mats (0.8 MPa to 3.2 MPa).
31349414	15	1	theme	good	1816:1819	arg1	fibres					1779:1784	electrospun fibres	1767:1784	electrospun fibres of CS-based polyurethanes	1767:1810	Results suggest that electrospun fibres of CS-based polyurethanes are good candidate scaffolds for soft tissue engineering.
31349414	15	1	theme	good	1816:1819	arg1	scaffolds					1831:1839	good candidate scaffolds	1816:1839	good candidate scaffolds for soft tissue engineering	1816:1867	Results suggest that electrospun fibres of CS-based polyurethanes are good candidate scaffolds for soft tissue engineering.
31349414	5	2	theme	microscopy	671:680	arg1	images					682:687	Scanning electron microscopy images	653:687	Scanning electron microscopy images of the electrospun scaffolds	653:716	Scanning electron microscopy images of the electrospun scaffolds revealed fibres with diameters around 1 μm.
31349414	3	3	theme	nuclear	517:523	arg1	resonance					534:542	proton nuclear magnetic resonance	510:542	proton nuclear magnetic resonance	510:542	Fourier transform infrared spectroscopy and proton nuclear magnetic resonance confirmed the syntheses.
31349414	8	4	theme	corresponding	1117:1129	arg1	films					1131:1135	the corresponding films	1113:1135	the corresponding films	1113:1135	From X-ray diffraction, a higher crystalline degree was identified in fibre mats than in the corresponding films.
31349414	15	5	theme	polyurethanes	1798:1810	arg1	fibres					1779:1784	electrospun fibres	1767:1784	electrospun fibres of CS-based polyurethanes	1767:1810	Results suggest that electrospun fibres of CS-based polyurethanes are good candidate scaffolds for soft tissue engineering.
31349414	15	5	theme	polyurethanes	1798:1810	arg1	scaffolds					1831:1839	good candidate scaffolds	1816:1839	good candidate scaffolds for soft tissue engineering	1816:1867	Results suggest that electrospun fibres of CS-based polyurethanes are good candidate scaffolds for soft tissue engineering.
31349414	2	6	theme	fibrous	280:286	arg1	structure					288:296	a porous electrospun fibrous structure	259:296	a porous electrospun fibrous structure from biodegradable polyurethanes (Pus) synthesized using polycaprolactone-diol as soft segment and, as chain extenders, chitosan (CS) and/or dimethylol propionic acid	259:463	In this work, we demonstrate the feasibility of obtaining a porous electrospun fibrous structure from biodegradable polyurethanes (Pus) synthesized using polycaprolactone-diol as soft segment and, as chain extenders, chitosan (CS) and/or dimethylol propionic acid.
31349414	2	7	theme	porous	261:266	arg1	structure					288:296	a porous electrospun fibrous structure	259:296	a porous electrospun fibrous structure from biodegradable polyurethanes (Pus) synthesized using polycaprolactone-diol as soft segment and, as chain extenders, chitosan (CS) and/or dimethylol propionic acid	259:463	In this work, we demonstrate the feasibility of obtaining a porous electrospun fibrous structure from biodegradable polyurethanes (Pus) synthesized using polycaprolactone-diol as soft segment and, as chain extenders, chitosan (CS) and/or dimethylol propionic acid.
31349414	5	8	theme	Scanning	653:660	arg1	microscopy					671:680	Scanning electron microscopy	653:680	Scanning electron microscopy images of the electrospun scaffolds	653:716	Scanning electron microscopy images of the electrospun scaffolds revealed fibres with diameters around 1 μm.
31349414	6	9	theme	Young	796:800	arg1	modulus					804:810	Young's modulus	796:810	Young's modulus	796:810	From tensile tests, we found that Young's modulus increases with CS content and is higher for films (2.5 MPa to 6.5 MPa) than for the corresponding fibre mats (0.8 MPa to 3.2 MPa).
31349414	10	10	from	present	1370:1376	arg1	concentrations					1389:1402	similar concentrations	1381:1402	similar concentrations at fibres' surface	1381:1421	X-ray photoelectron spectroscopy revealed that while ester groups are predominant at the films' surface, ester and urethanes are present in similar concentrations at fibres' surface, favouring the interaction with water molecules.
31349414	12	11	theme	human	1565:1569	arg1	fibroblasts					1578:1588	human dermal fibroblasts	1565:1588	human dermal fibroblasts	1565:1588	In vitro evaluation was performed with human dermal fibroblasts.
31349414	10	12	located	present	1370:1376	arg2	urethanes					1356:1364	urethanes	1356:1364	urethanes	1356:1364	X-ray photoelectron spectroscopy revealed that while ester groups are predominant at the films' surface, ester and urethanes are present in similar concentrations at fibres' surface, favouring the interaction with water molecules.
31349414	10	12	located	present	1370:1376	arg1	concentrations					1389:1402	similar concentrations	1381:1402	similar concentrations at fibres' surface	1381:1421	X-ray photoelectron spectroscopy revealed that while ester groups are predominant at the films' surface, ester and urethanes are present in similar concentrations at fibres' surface, favouring the interaction with water molecules.
31349414	10	12	located	present	1370:1376	arg2	ester					1346:1350	ester	1346:1350	ester	1346:1350	X-ray photoelectron spectroscopy revealed that while ester groups are predominant at the films' surface, ester and urethanes are present in similar concentrations at fibres' surface, favouring the interaction with water molecules.
31349414	15	13	theme	CS-based	1789:1796	arg1	polyurethanes					1798:1810	CS-based polyurethanes	1789:1810	CS-based polyurethanes	1789:1810	Results suggest that electrospun fibres of CS-based polyurethanes are good candidate scaffolds for soft tissue engineering.
31349414	15	14	theme	soft	1845:1848	arg1	engineering					1857:1867	soft tissue engineering	1845:1867	soft tissue engineering	1845:1867	Results suggest that electrospun fibres of CS-based polyurethanes are good candidate scaffolds for soft tissue engineering.
31349414	5	15	theme	electrospun	696:706	arg1	scaffolds					708:716	the electrospun scaffolds	692:716	the electrospun scaffolds	692:716	Scanning electron microscopy images of the electrospun scaffolds revealed fibres with diameters around 1 μm.
31349414	14	16	theme	fibre	1644:1648	arg1	mats					1650:1653	fibre mats	1644:1653	fibre mats	1644:1653	Cells adhered to fibre mats better than to films and proliferation was observed only for samples of CS-containing PUs.
31349414	7	17	theme	only	964:967	arg1	extender					975:982	the only chain extender	960:982	the only chain extender	960:982	The use of CS as the only chain extender improves recovery ratio and resilience.
31349414	14	18	theme	adhered	1633:1639	arg1	Cells					1627:1631	Cells	1627:1631	Cells adhered to fibre mats better than to films and proliferation	1627:1692	Cells adhered to fibre mats better than to films and proliferation was observed only for samples of CS-containing PUs.
31349414	10	19	with	interaction	1438:1448	arg1	molecules					1461:1469	water molecules	1455:1469	water molecules	1455:1469	X-ray photoelectron spectroscopy revealed that while ester groups are predominant at the films' surface, ester and urethanes are present in similar concentrations at fibres' surface, favouring the interaction with water molecules.
31349414	9	20	theme	water	1220:1224	arg1	angle					1234:1238	water contact angle	1220:1238	water contact angle	1220:1238	Films' wettability was enhanced by the presence of CS as shown by the decrease of water contact angle.
31349414	1	21	from	role	172:175	arg1	regeneration					187:198	tissue regeneration	180:198	tissue regeneration	180:198	The composition and architecture of a scaffold determine its supportive role in tissue regeneration.
31349414	0	22	theme	soft	75:78	arg1	engineering					87:97	soft tissue engineering	75:97	soft tissue engineering	75:97	Electrospun biodegradable chitosan based-poly(urethane urea) scaffolds for soft tissue engineering.
31349414	9	23	theme	angle	1234:1238	arg1	decrease					1208:1215	the decrease	1204:1215	the decrease of water contact angle	1204:1238	Films' wettability was enhanced by the presence of CS as shown by the decrease of water contact angle.
31349414	1	24	theme	supportive	161:170	arg1	role					172:175	its supportive role	157:175	its supportive role in tissue regeneration	157:198	The composition and architecture of a scaffold determine its supportive role in tissue regeneration.
31349414	15	25	theme	electrospun	1767:1777	arg1	fibres					1779:1784	electrospun fibres	1767:1784	electrospun fibres of CS-based polyurethanes	1767:1810	Results suggest that electrospun fibres of CS-based polyurethanes are good candidate scaffolds for soft tissue engineering.
31349414	15	25	theme	electrospun	1767:1777	arg1	scaffolds					1831:1839	good candidate scaffolds	1816:1839	good candidate scaffolds for soft tissue engineering	1816:1867	Results suggest that electrospun fibres of CS-based polyurethanes are good candidate scaffolds for soft tissue engineering.
31349414	2	26	theme	chain	401:405	arg1	polycaprolactone-diol					355:375	polycaprolactone-diol	355:375	polycaprolactone-diol	355:375	In this work, we demonstrate the feasibility of obtaining a porous electrospun fibrous structure from biodegradable polyurethanes (Pus) synthesized using polycaprolactone-diol as soft segment and, as chain extenders, chitosan (CS) and/or dimethylol propionic acid.
31349414	2	26	theme	chain	401:405	arg1	extenders					407:415	chain extenders	401:415	chain extenders	401:415	In this work, we demonstrate the feasibility of obtaining a porous electrospun fibrous structure from biodegradable polyurethanes (Pus) synthesized using polycaprolactone-diol as soft segment and, as chain extenders, chitosan (CS) and/or dimethylol propionic acid.
31349414	2	27	theme	dimethylol	439:448	arg1	acid					460:463	dimethylol propionic acid	439:463	dimethylol propionic acid	439:463	In this work, we demonstrate the feasibility of obtaining a porous electrospun fibrous structure from biodegradable polyurethanes (Pus) synthesized using polycaprolactone-diol as soft segment and, as chain extenders, chitosan (CS) and/or dimethylol propionic acid.
31349414	0	28	theme	Electrospun	0:10	arg1	based-poly					35:44	Electrospun biodegradable chitosan based-poly	0:44	Electrospun biodegradable chitosan based-poly(urethane urea)	0:59	Electrospun biodegradable chitosan based-poly(urethane urea) scaffolds for soft tissue engineering.
31349414	0	28	theme	Electrospun	0:10	arg1	urea					55:58	urethane urea	46:58	urethane urea	46:58	Electrospun biodegradable chitosan based-poly(urethane urea) scaffolds for soft tissue engineering.
31349414	8	29	theme	X-ray	1029:1033	arg1	diffraction					1035:1045	X-ray diffraction	1029:1045	X-ray diffraction	1029:1045	From X-ray diffraction, a higher crystalline degree was identified in fibre mats than in the corresponding films.
31349414	4	30	theme	Fibre	569:573	arg1	properties					581:590	Fibre mats' properties	569:590	Fibre mats' properties	569:590	Fibre mats' properties were analysed and compared with those of solvent cast films.
31349414	0	31	theme	chitosan	26:33	arg1	based-poly					35:44	Electrospun biodegradable chitosan based-poly	0:44	Electrospun biodegradable chitosan based-poly(urethane urea)	0:59	Electrospun biodegradable chitosan based-poly(urethane urea) scaffolds for soft tissue engineering.
31349414	0	31	theme	chitosan	26:33	arg1	urea					55:58	urethane urea	46:58	urethane urea	46:58	Electrospun biodegradable chitosan based-poly(urethane urea) scaffolds for soft tissue engineering.
31349414	10	32	theme	X-ray	1241:1245	arg1	spectroscopy					1261:1272	X-ray photoelectron spectroscopy	1241:1272	X-ray photoelectron spectroscopy	1241:1272	X-ray photoelectron spectroscopy revealed that while ester groups are predominant at the films' surface, ester and urethanes are present in similar concentrations at fibres' surface, favouring the interaction with water molecules.
31349414	10	33	attach	present	1370:1376	arg2	urethanes					1356:1364	urethanes	1356:1364	urethanes	1356:1364	X-ray photoelectron spectroscopy revealed that while ester groups are predominant at the films' surface, ester and urethanes are present in similar concentrations at fibres' surface, favouring the interaction with water molecules.
31349414	10	33	attach	present	1370:1376	arg1	concentrations					1389:1402	similar concentrations	1381:1402	similar concentrations at fibres' surface	1381:1421	X-ray photoelectron spectroscopy revealed that while ester groups are predominant at the films' surface, ester and urethanes are present in similar concentrations at fibres' surface, favouring the interaction with water molecules.
31349414	10	33	attach	present	1370:1376	arg2	ester					1346:1350	ester	1346:1350	ester	1346:1350	X-ray photoelectron spectroscopy revealed that while ester groups are predominant at the films' surface, ester and urethanes are present in similar concentrations at fibres' surface, favouring the interaction with water molecules.
31349414	8	34	theme	higher	1050:1055	arg1	degree					1069:1074	a higher crystalline degree	1048:1074	a higher crystalline degree	1048:1074	From X-ray diffraction, a higher crystalline degree was identified in fibre mats than in the corresponding films.
31349414	8	35	from	mats	1100:1103	arg1	films					1131:1135	the corresponding films	1113:1135	the corresponding films	1113:1135	From X-ray diffraction, a higher crystalline degree was identified in fibre mats than in the corresponding films.
31349414	10	36	theme	ester	1294:1298	arg1	groups					1300:1305	ester groups	1294:1305	ester groups	1294:1305	X-ray photoelectron spectroscopy revealed that while ester groups are predominant at the films' surface, ester and urethanes are present in similar concentrations at fibres' surface, favouring the interaction with water molecules.
31349414	14	37	theme	CS-containing	1727:1739	arg1	PUs					1741:1743	CS-containing PUs	1727:1743	CS-containing PUs	1727:1743	Cells adhered to fibre mats better than to films and proliferation was observed only for samples of CS-containing PUs.
31349414	10	38	theme	water	1455:1459	arg1	molecules					1461:1469	water molecules	1455:1469	water molecules	1455:1469	X-ray photoelectron spectroscopy revealed that while ester groups are predominant at the films' surface, ester and urethanes are present in similar concentrations at fibres' surface, favouring the interaction with water molecules.
31349414	10	39	from	surface	1337:1343	arg1	predominant					1311:1321	predominant	1311:1321	predominant	1311:1321	X-ray photoelectron spectroscopy revealed that while ester groups are predominant at the films' surface, ester and urethanes are present in similar concentrations at fibres' surface, favouring the interaction with water molecules.
31349414	6	40	dep	6.5 MPa	874:880	arg1	to					871:872	to	871:872	to	871:872	From tensile tests, we found that Young's modulus increases with CS content and is higher for films (2.5 MPa to 6.5 MPa) than for the corresponding fibre mats (0.8 MPa to 3.2 MPa).
31349414	15	41	theme	candidate	1821:1829	arg1	fibres					1779:1784	electrospun fibres	1767:1784	electrospun fibres of CS-based polyurethanes	1767:1810	Results suggest that electrospun fibres of CS-based polyurethanes are good candidate scaffolds for soft tissue engineering.
31349414	15	41	theme	candidate	1821:1829	arg1	scaffolds					1831:1839	good candidate scaffolds	1816:1839	good candidate scaffolds for soft tissue engineering	1816:1867	Results suggest that electrospun fibres of CS-based polyurethanes are good candidate scaffolds for soft tissue engineering.
31349414	3	42	theme	magnetic	525:532	arg1	resonance					534:542	proton nuclear magnetic resonance	510:542	proton nuclear magnetic resonance	510:542	Fourier transform infrared spectroscopy and proton nuclear magnetic resonance confirmed the syntheses.
31349414	8	43	theme	fibre	1094:1098	arg1	mats					1100:1103	fibre mats	1094:1103	fibre mats than in the corresponding films	1094:1135	From X-ray diffraction, a higher crystalline degree was identified in fibre mats than in the corresponding films.
31349414	3	44	dep	Fourier	466:472	arg1	transform					474:482	transform	474:482	transform infrared spectroscopy and proton nuclear magnetic resonance	474:542	Fourier transform infrared spectroscopy and proton nuclear magnetic resonance confirmed the syntheses.
31349414	5	45	theme	electron	662:669	arg1	microscopy					671:680	Scanning electron microscopy	653:680	Scanning electron microscopy images of the electrospun scaffolds	653:716	Scanning electron microscopy images of the electrospun scaffolds revealed fibres with diameters around 1 μm.
31349414	3	46	theme	proton	510:515	arg1	resonance					534:542	proton nuclear magnetic resonance	510:542	proton nuclear magnetic resonance	510:542	Fourier transform infrared spectroscopy and proton nuclear magnetic resonance confirmed the syntheses.
31349414	9	47	theme	contact	1226:1232	arg1	angle					1234:1238	water contact angle	1220:1238	water contact angle	1220:1238	Films' wettability was enhanced by the presence of CS as shown by the decrease of water contact angle.
31349414	6	48	theme	corresponding	896:908	arg1	3.2 MPa					933:939	0.8 MPa to 3.2 MPa	922:939	0.8 MPa to 3.2 MPa	922:939	From tensile tests, we found that Young's modulus increases with CS content and is higher for films (2.5 MPa to 6.5 MPa) than for the corresponding fibre mats (0.8 MPa to 3.2 MPa).
31349414	6	48	theme	corresponding	896:908	arg1	mats					916:919	the corresponding fibre mats	892:919	the corresponding fibre mats (0.8 MPa to 3.2 MPa)	892:940	From tensile tests, we found that Young's modulus increases with CS content and is higher for films (2.5 MPa to 6.5 MPa) than for the corresponding fibre mats (0.8 MPa to 3.2 MPa).
31349414	7	49	dep	recovery	993:1000	arg1	ratio					1002:1006	ratio	1002:1006	ratio	1002:1006	The use of CS as the only chain extender improves recovery ratio and resilience.
31349414	12	50	dep	In	1526:1527	arg1	vitro					1529:1533	vitro	1529:1533	vitro	1529:1533	In vitro evaluation was performed with human dermal fibroblasts.
31349414	6	51	theme	CS	827:828	arg1	content					830:836	CS content	827:836	CS content	827:836	From tensile tests, we found that Young's modulus increases with CS content and is higher for films (2.5 MPa to 6.5 MPa) than for the corresponding fibre mats (0.8 MPa to 3.2 MPa).
31349414	2	52	theme	electrospun	268:278	arg1	structure					288:296	a porous electrospun fibrous structure	259:296	a porous electrospun fibrous structure from biodegradable polyurethanes (Pus) synthesized using polycaprolactone-diol as soft segment and, as chain extenders, chitosan (CS) and/or dimethylol propionic acid	259:463	In this work, we demonstrate the feasibility of obtaining a porous electrospun fibrous structure from biodegradable polyurethanes (Pus) synthesized using polycaprolactone-diol as soft segment and, as chain extenders, chitosan (CS) and/or dimethylol propionic acid.
31349414	1	53	theme	tissue	180:185	arg1	regeneration					187:198	tissue regeneration	180:198	tissue regeneration	180:198	The composition and architecture of a scaffold determine its supportive role in tissue regeneration.
31349414	11	54	theme	hydrolytic	1502:1511	arg1	degradation					1513:1523	hydrolytic degradation	1502:1523	hydrolytic degradation	1502:1523	Both films and fibres undergo hydrolytic degradation.
31349414	2	55	theme	biodegradable	303:315	arg1	Pus					332:334	Pus	332:334	Pus	332:334	In this work, we demonstrate the feasibility of obtaining a porous electrospun fibrous structure from biodegradable polyurethanes (Pus) synthesized using polycaprolactone-diol as soft segment and, as chain extenders, chitosan (CS) and/or dimethylol propionic acid.
31349414	2	55	theme	biodegradable	303:315	arg1	polyurethanes					317:329	biodegradable polyurethanes	303:329	biodegradable polyurethanes (Pus) synthesized using polycaprolactone-diol as soft segment and, as chain extenders, chitosan (CS) and/or dimethylol propionic acid	303:463	In this work, we demonstrate the feasibility of obtaining a porous electrospun fibrous structure from biodegradable polyurethanes (Pus) synthesized using polycaprolactone-diol as soft segment and, as chain extenders, chitosan (CS) and/or dimethylol propionic acid.
31349414	12	56	theme	dermal	1571:1576	arg1	fibroblasts					1578:1588	human dermal fibroblasts	1565:1588	human dermal fibroblasts	1565:1588	In vitro evaluation was performed with human dermal fibroblasts.
31349414	4	57	theme	cast	641:644	arg1	films					646:650	solvent cast films	633:650	solvent cast films	633:650	Fibre mats' properties were analysed and compared with those of solvent cast films.
31349414	15	58	theme	tissue	1850:1855	arg1	engineering					1857:1867	soft tissue engineering	1845:1867	soft tissue engineering	1845:1867	Results suggest that electrospun fibres of CS-based polyurethanes are good candidate scaffolds for soft tissue engineering.
31349414	10	59	attach	predominant	1311:1321	arg1	surface					1337:1343	the films' surface	1326:1343	the films' surface	1326:1343	X-ray photoelectron spectroscopy revealed that while ester groups are predominant at the films' surface, ester and urethanes are present in similar concentrations at fibres' surface, favouring the interaction with water molecules.
31349414	10	59	attach	predominant	1311:1321	arg2	groups					1300:1305	ester groups	1294:1305	ester groups	1294:1305	X-ray photoelectron spectroscopy revealed that while ester groups are predominant at the films' surface, ester and urethanes are present in similar concentrations at fibres' surface, favouring the interaction with water molecules.
31349414	9	60	theme	CS	1189:1190	arg1	presence					1177:1184	the presence	1173:1184	the presence of CS	1173:1190	Films' wettability was enhanced by the presence of CS as shown by the decrease of water contact angle.
31349414	6	61	dep	3.2 MPa	933:939	arg1	to					930:931	to	930:931	to	930:931	From tensile tests, we found that Young's modulus increases with CS content and is higher for films (2.5 MPa to 6.5 MPa) than for the corresponding fibre mats (0.8 MPa to 3.2 MPa).
31349414	6	62	theme	0.8 MPa	922:928	arg1	3.2 MPa					933:939	0.8 MPa to 3.2 MPa	922:939	0.8 MPa to 3.2 MPa	922:939	From tensile tests, we found that Young's modulus increases with CS content and is higher for films (2.5 MPa to 6.5 MPa) than for the corresponding fibre mats (0.8 MPa to 3.2 MPa).
31349414	6	62	theme	0.8 MPa	922:928	arg1	mats					916:919	the corresponding fibre mats	892:919	the corresponding fibre mats (0.8 MPa to 3.2 MPa)	892:940	From tensile tests, we found that Young's modulus increases with CS content and is higher for films (2.5 MPa to 6.5 MPa) than for the corresponding fibre mats (0.8 MPa to 3.2 MPa).
31349414	5	63	theme	scaffolds	708:716	arg1	images					682:687	Scanning electron microscopy images	653:687	Scanning electron microscopy images of the electrospun scaffolds	653:716	Scanning electron microscopy images of the electrospun scaffolds revealed fibres with diameters around 1 μm.
31349414	7	64	theme	chain	969:973	arg1	extender					975:982	the only chain extender	960:982	the only chain extender	960:982	The use of CS as the only chain extender improves recovery ratio and resilience.
31349414	1	65	theme	scaffold	138:145	arg1	architecture					120:131	architecture	120:131	architecture	120:131	The composition and architecture of a scaffold determine its supportive role in tissue regeneration.
31349414	1	65	theme	scaffold	138:145	arg1	composition					104:114	composition	104:114	composition	104:114	The composition and architecture of a scaffold determine its supportive role in tissue regeneration.
31349414	1	66	dep	composition	104:114	arg1	The					100:102	The	100:102	The	100:102	The composition and architecture of a scaffold determine its supportive role in tissue regeneration.
31349414	0	67	theme	tissue	80:85	arg1	engineering					87:97	soft tissue engineering	75:97	soft tissue engineering	75:97	Electrospun biodegradable chitosan based-poly(urethane urea) scaffolds for soft tissue engineering.
31349414	10	68	from	concentrations	1389:1402	arg1	present					1370:1376	present	1370:1376	present	1370:1376	X-ray photoelectron spectroscopy revealed that while ester groups are predominant at the films' surface, ester and urethanes are present in similar concentrations at fibres' surface, favouring the interaction with water molecules.
31349414	7	69	theme	CS	954:955	arg1	use					947:949	The use	943:949	The use of CS as the only chain extender	943:982	The use of CS as the only chain extender improves recovery ratio and resilience.
31349414	10	70	theme	similar	1381:1387	arg1	concentrations					1389:1402	similar concentrations	1381:1402	similar concentrations at fibres' surface	1381:1421	X-ray photoelectron spectroscopy revealed that while ester groups are predominant at the films' surface, ester and urethanes are present in similar concentrations at fibres' surface, favouring the interaction with water molecules.
31349414	3	71	dep	transform	474:482	arg1	infrared					484:491	infrared	484:491	transform infrared spectroscopy and proton nuclear magnetic resonance	474:542	Fourier transform infrared spectroscopy and proton nuclear magnetic resonance confirmed the syntheses.
31349414	2	72	from	polyurethanes	317:329	arg1	structure					288:296	a porous electrospun fibrous structure	259:296	a porous electrospun fibrous structure from biodegradable polyurethanes (Pus) synthesized using polycaprolactone-diol as soft segment and, as chain extenders, chitosan (CS) and/or dimethylol propionic acid	259:463	In this work, we demonstrate the feasibility of obtaining a porous electrospun fibrous structure from biodegradable polyurethanes (Pus) synthesized using polycaprolactone-diol as soft segment and, as chain extenders, chitosan (CS) and/or dimethylol propionic acid.
31349414	0	73	theme	biodegradable	12:24	arg1	based-poly					35:44	Electrospun biodegradable chitosan based-poly	0:44	Electrospun biodegradable chitosan based-poly(urethane urea)	0:59	Electrospun biodegradable chitosan based-poly(urethane urea) scaffolds for soft tissue engineering.
31349414	0	73	theme	biodegradable	12:24	arg1	urea					55:58	urethane urea	46:58	urethane urea	46:58	Electrospun biodegradable chitosan based-poly(urethane urea) scaffolds for soft tissue engineering.
31349414	10	74	theme	photoelectron	1247:1259	arg1	spectroscopy					1261:1272	X-ray photoelectron spectroscopy	1241:1272	X-ray photoelectron spectroscopy	1241:1272	X-ray photoelectron spectroscopy revealed that while ester groups are predominant at the films' surface, ester and urethanes are present in similar concentrations at fibres' surface, favouring the interaction with water molecules.
31349414	0	75	theme	urethane	46:53	arg1	based-poly					35:44	Electrospun biodegradable chitosan based-poly	0:44	Electrospun biodegradable chitosan based-poly(urethane urea)	0:59	Electrospun biodegradable chitosan based-poly(urethane urea) scaffolds for soft tissue engineering.
31349414	0	75	theme	urethane	46:53	arg1	urea					55:58	urethane urea	46:58	urethane urea	46:58	Electrospun biodegradable chitosan based-poly(urethane urea) scaffolds for soft tissue engineering.
31349414	8	76	theme	crystalline	1057:1067	arg1	degree					1069:1074	a higher crystalline degree	1048:1074	a higher crystalline degree	1048:1074	From X-ray diffraction, a higher crystalline degree was identified in fibre mats than in the corresponding films.
31349414	6	77	theme	2.5 MPa	863:869	arg1	6.5 MPa					874:880	2.5 MPa to 6.5 MPa	863:880	2.5 MPa to 6.5 MPa	863:880	From tensile tests, we found that Young's modulus increases with CS content and is higher for films (2.5 MPa to 6.5 MPa) than for the corresponding fibre mats (0.8 MPa to 3.2 MPa).
31349414	6	77	theme	2.5 MPa	863:869	arg1	films					856:860	films	856:860	films (2.5 MPa to 6.5 MPa)	856:881	From tensile tests, we found that Young's modulus increases with CS content and is higher for films (2.5 MPa to 6.5 MPa) than for the corresponding fibre mats (0.8 MPa to 3.2 MPa).
31349414	5	78	with	fibres	727:732	arg1	diameters					739:747	diameters	739:747	diameters around 1 μm	739:759	Scanning electron microscopy images of the electrospun scaffolds revealed fibres with diameters around 1 μm.
31349414	14	79	theme	PUs	1741:1743	arg1	samples					1716:1722	samples	1716:1722	samples of CS-containing PUs	1716:1743	Cells adhered to fibre mats better than to films and proliferation was observed only for samples of CS-containing PUs.
31349414	2	80	theme	propionic	450:458	arg1	acid					460:463	dimethylol propionic acid	439:463	dimethylol propionic acid	439:463	In this work, we demonstrate the feasibility of obtaining a porous electrospun fibrous structure from biodegradable polyurethanes (Pus) synthesized using polycaprolactone-diol as soft segment and, as chain extenders, chitosan (CS) and/or dimethylol propionic acid.
31349414	14	81	dep	better	1655:1660	arg1	than					1662:1665	than	1662:1665	than	1662:1665	Cells adhered to fibre mats better than to films and proliferation was observed only for samples of CS-containing PUs.
31349414	13	82	theme	PU	1594:1595	arg1	sample					1597:1602	No PU sample	1591:1602	No PU sample	1591:1602	No PU sample revealed cytotoxicity.
31349414	6	83	theme	tensile	767:773	arg1	tests					775:779	tensile tests	767:779	tensile tests	767:779	From tensile tests, we found that Young's modulus increases with CS content and is higher for films (2.5 MPa to 6.5 MPa) than for the corresponding fibre mats (0.8 MPa to 3.2 MPa).
31349414	4	84	theme	solvent	633:639	arg1	films					646:650	solvent cast films	633:650	solvent cast films	633:650	Fibre mats' properties were analysed and compared with those of solvent cast films.
31349414	2	85	theme	soft	380:383	arg1	segment					385:391	soft segment	380:391	soft segment	380:391	In this work, we demonstrate the feasibility of obtaining a porous electrospun fibrous structure from biodegradable polyurethanes (Pus) synthesized using polycaprolactone-diol as soft segment and, as chain extenders, chitosan (CS) and/or dimethylol propionic acid.
31349414	12	86	theme	In	1526:1527	arg1	evaluation					1535:1544	In vitro evaluation	1526:1544	In vitro evaluation	1526:1544	In vitro evaluation was performed with human dermal fibroblasts.
30896386	6	0	theme	NRRL	824:827	arg1	%					843:843	99.0 %	838:843	99.0 %	838:843	Phylogenetic analysis based on its 16S rRNA gene sequence indicated that the novel strain should be assigned to the genus Streptomyces, and the closest species was Streptomyces puniciscabiei S77T with 99.1 % sequence similarity, which was followed by Streptomyces durhamensis NRRL B-3309T (99.0 %), Streptomyces filipinensis NBRC 12860T (98.9 %) and Streptomyces yaanensis Z4T (98.7 %).
30896386	6	0	theme	NRRL	824:827	arg1	B-3309T					829:835	Streptomyces durhamensis NRRL B-3309T	799:835	Streptomyces durhamensis NRRL B-3309T (99.0 %)	799:844	Phylogenetic analysis based on its 16S rRNA gene sequence indicated that the novel strain should be assigned to the genus Streptomyces, and the closest species was Streptomyces puniciscabiei S77T with 99.1 % sequence similarity, which was followed by Streptomyces durhamensis NRRL B-3309T (99.0 %), Streptomyces filipinensis NBRC 12860T (98.9 %) and Streptomyces yaanensis Z4T (98.7 %).
30896386	11	1	theme	neighbouring	1485:1496	arg1	species					1498:1504	its neighbouring species	1481:1504	its neighbouring species	1481:1504	However, strain TW1S1T could be distinguished from its neighbouring species by its phenotypic properties.
30896386	6	2	theme	Streptomyces	712:723	arg1	species					700:706	the closest species	688:706	the closest species	688:706	Phylogenetic analysis based on its 16S rRNA gene sequence indicated that the novel strain should be assigned to the genus Streptomyces, and the closest species was Streptomyces puniciscabiei S77T with 99.1 % sequence similarity, which was followed by Streptomyces durhamensis NRRL B-3309T (99.0 %), Streptomyces filipinensis NBRC 12860T (98.9 %) and Streptomyces yaanensis Z4T (98.7 %).
30896386	6	2	theme	Streptomyces	712:723	arg1	S77T					739:742	Streptomyces puniciscabiei S77T	712:742	Streptomyces puniciscabiei S77T	712:742	Phylogenetic analysis based on its 16S rRNA gene sequence indicated that the novel strain should be assigned to the genus Streptomyces, and the closest species was Streptomyces puniciscabiei S77T with 99.1 % sequence similarity, which was followed by Streptomyces durhamensis NRRL B-3309T (99.0 %), Streptomyces filipinensis NBRC 12860T (98.9 %) and Streptomyces yaanensis Z4T (98.7 %).
30896386	8	3	theme	diamino	1051:1057	arg1	acid					1059:1062	the diagnostic diamino acid	1036:1062	the diagnostic diamino acid	1036:1062	ll-Diaminopimelic acid was the diagnostic diamino acid, and alanine, glutamic acid and glycine were present in the peptidoglycan.
30896386	8	3	theme	diamino	1051:1057	arg1	acid					1027:1030	ll-Diaminopimelic acid	1009:1030	ll-Diaminopimelic acid	1009:1030	ll-Diaminopimelic acid was the diagnostic diamino acid, and alanine, glutamic acid and glycine were present in the peptidoglycan.
30896386	6	4	theme	Streptomyces	799:810	arg1	%					843:843	99.0 %	838:843	99.0 %	838:843	Phylogenetic analysis based on its 16S rRNA gene sequence indicated that the novel strain should be assigned to the genus Streptomyces, and the closest species was Streptomyces puniciscabiei S77T with 99.1 % sequence similarity, which was followed by Streptomyces durhamensis NRRL B-3309T (99.0 %), Streptomyces filipinensis NBRC 12860T (98.9 %) and Streptomyces yaanensis Z4T (98.7 %).
30896386	6	4	theme	Streptomyces	799:810	arg1	B-3309T					829:835	Streptomyces durhamensis NRRL B-3309T	799:835	Streptomyces durhamensis NRRL B-3309T (99.0 %)	799:844	Phylogenetic analysis based on its 16S rRNA gene sequence indicated that the novel strain should be assigned to the genus Streptomyces, and the closest species was Streptomyces puniciscabiei S77T with 99.1 % sequence similarity, which was followed by Streptomyces durhamensis NRRL B-3309T (99.0 %), Streptomyces filipinensis NBRC 12860T (98.9 %) and Streptomyces yaanensis Z4T (98.7 %).
30896386	10	5	theme	isoprenoid	1236:1245	arg1	quinones					1247:1254	The predominant isoprenoid quinones	1220:1254	The predominant isoprenoid quinones	1220:1254	The predominant isoprenoid quinones were MK-9(H4) and MK-9(H6), the major polar lipids were phosphatidylglycerol and an unidentified phospholipid, and the main fatty acids were iso-C16 : 0 and anteiso-C15 : 0.
30896386	10	5	theme	isoprenoid	1236:1245	arg1	MK-9					1261:1264	MK-9	1261:1264	MK-9	1261:1264	The predominant isoprenoid quinones were MK-9(H4) and MK-9(H6), the major polar lipids were phosphatidylglycerol and an unidentified phospholipid, and the main fatty acids were iso-C16 : 0 and anteiso-C15 : 0.
30896386	2	6	theme	antifungal	150:159	arg1	substance					161:169	an antifungal substance	147:169	an antifungal substance	147:169	A novel actinobacterial strain producing an antifungal substance was isolated from a sample of acidic mine area soil, and its taxonomic position was evaluated.
30896386	8	7	theme	ll-Diaminopimelic	1009:1025	arg1	acid					1059:1062	the diagnostic diamino acid	1036:1062	the diagnostic diamino acid	1036:1062	ll-Diaminopimelic acid was the diagnostic diamino acid, and alanine, glutamic acid and glycine were present in the peptidoglycan.
30896386	8	7	theme	ll-Diaminopimelic	1009:1025	arg1	acid					1027:1030	ll-Diaminopimelic acid	1009:1030	ll-Diaminopimelic acid	1009:1030	ll-Diaminopimelic acid was the diagnostic diamino acid, and alanine, glutamic acid and glycine were present in the peptidoglycan.
30896386	12	8	theme	strain	1617:1622	arg1	species					1666:1672	a separate species	1655:1672	a separate species	1655:1672	In addition, the genome-based comparison with the closest species indicated that strain TW1S1T should be recognized as a separate species.
30896386	12	8	theme	strain	1617:1622	arg1	TW1S1T					1624:1629	strain TW1S1T	1617:1629	strain TW1S1T	1617:1629	In addition, the genome-based comparison with the closest species indicated that strain TW1S1T should be recognized as a separate species.
30896386	5	9	theme	extract-iron	529:540	arg1	agar					542:545	peptone-yeast extract-iron agar	515:545	peptone-yeast extract-iron agar	515:545	Melanin was produced on peptone-yeast extract-iron agar.
30896386	13	10	theme	novel	1786:1790	arg1	species					1792:1798	a novel species	1784:1798	a novel species	1784:1798	The phylogenetic, phenotypic and chemotaxonomic as well as genomic evidence supported that TW1S1T represents a novel species of Streptomyces, for which the name Streptomycesfodineus sp.
30896386	10	11	dep	MK-9	1261:1264	arg1	 0					1426:1427	 0	1426:1427	 0	1426:1427	The predominant isoprenoid quinones were MK-9(H4) and MK-9(H6), the major polar lipids were phosphatidylglycerol and an unidentified phospholipid, and the main fatty acids were iso-C16 : 0 and anteiso-C15 : 0.
30896386	10	11	dep	MK-9	1261:1264	arg1	anteiso-C15 					1413:1424	anteiso-C15 	1413:1424	anteiso-C15 	1413:1424	The predominant isoprenoid quinones were MK-9(H4) and MK-9(H6), the major polar lipids were phosphatidylglycerol and an unidentified phospholipid, and the main fatty acids were iso-C16 : 0 and anteiso-C15 : 0.
30896386	10	11	dep	MK-9	1261:1264	arg1	 0					1406:1407	 0	1406:1407	 0	1406:1407	The predominant isoprenoid quinones were MK-9(H4) and MK-9(H6), the major polar lipids were phosphatidylglycerol and an unidentified phospholipid, and the main fatty acids were iso-C16 : 0 and anteiso-C15 : 0.
30896386	8	12	theme	glutamic	1078:1085	arg1	acid					1087:1090	glutamic acid	1078:1090	glutamic acid	1078:1090	ll-Diaminopimelic acid was the diagnostic diamino acid, and alanine, glutamic acid and glycine were present in the peptidoglycan.
30896386	14	13	dep	strain	1884:1889	arg1	32404T					1919:1924	TW1S1T = KCTC 49013T = JCM 32404T	1892:1924	TW1S1T = KCTC 49013T = JCM 32404T	1892:1924	nov. is proposed (type strain, TW1S1T = KCTC 49013T = JCM 32404T).
30896386	12	14	theme	closest	1586:1592	arg1	species					1594:1600	the closest species	1582:1600	the closest species	1582:1600	In addition, the genome-based comparison with the closest species indicated that strain TW1S1T should be recognized as a separate species.
30896386	7	15	theme	chemotaxonomic	939:952	arg1	consistent					970:979	consistent	970:979	consistent	970:979	The chemotaxonomic properties were consistent with those of Streptomyces.
30896386	7	15	theme	chemotaxonomic	939:952	arg1	properties					954:963	The chemotaxonomic properties	935:963	The chemotaxonomic properties	935:963	The chemotaxonomic properties were consistent with those of Streptomyces.
30896386	14	16	dep	proposed	1869:1876	arg1	strain					1884:1889	type strain	1879:1889	type strain	1879:1889	nov. is proposed (type strain, TW1S1T = KCTC 49013T = JCM 32404T).
30896386	14	17	theme	KCTC	1901:1904	arg1	32404T					1919:1924	TW1S1T = KCTC 49013T = JCM 32404T	1892:1924	TW1S1T = KCTC 49013T = JCM 32404T	1892:1924	nov. is proposed (type strain, TW1S1T = KCTC 49013T = JCM 32404T).
30896386	2	18	theme	soil	218:221	arg1	sample					191:196	a sample	189:196	a sample of acidic mine area soil	189:221	A novel actinobacterial strain producing an antifungal substance was isolated from a sample of acidic mine area soil, and its taxonomic position was evaluated.
30896386	8	19	from	peptidoglycan	1124:1136	arg1	present					1109:1115	present	1109:1115	present	1109:1115	ll-Diaminopimelic acid was the diagnostic diamino acid, and alanine, glutamic acid and glycine were present in the peptidoglycan.
30896386	2	20	theme	mine	208:211	arg1	soil					218:221	acidic mine area soil	201:221	acidic mine area soil	201:221	A novel actinobacterial strain producing an antifungal substance was isolated from a sample of acidic mine area soil, and its taxonomic position was evaluated.
30896386	3	21	theme	novel	270:274	arg1	strain					276:281	The novel strain	266:281	The novel strain	266:281	The novel strain, designated TW1S1T, formed white-grey aerial mycelium and yellow substrate mycelium on oatmeal agar.
30896386	9	22	theme	cell-wall	1143:1151	arg1	hydrolysate					1153:1163	The cell-wall hydrolysate	1139:1163	The cell-wall hydrolysate	1139:1163	The cell-wall hydrolysate also contained galactose, glucose, mannose and ribose.
30896386	2	23	theme	taxonomic	232:240	arg1	position					242:249	its taxonomic position	228:249	its taxonomic position	228:249	A novel actinobacterial strain producing an antifungal substance was isolated from a sample of acidic mine area soil, and its taxonomic position was evaluated.
30896386	13	24	theme	phylogenetic	1679:1690	arg1	evidence					1742:1749	The phylogenetic, phenotypic and chemotaxonomic as well as genomic evidence	1675:1749	evidence	1742:1749	The phylogenetic, phenotypic and chemotaxonomic as well as genomic evidence supported that TW1S1T represents a novel species of Streptomyces, for which the name Streptomycesfodineus sp.
30896386	6	25	theme	Streptomyces	898:909	arg1	Z4T					921:923	Streptomyces yaanensis Z4T	898:923	Streptomyces yaanensis Z4T (98.7 %)	898:932	Phylogenetic analysis based on its 16S rRNA gene sequence indicated that the novel strain should be assigned to the genus Streptomyces, and the closest species was Streptomyces puniciscabiei S77T with 99.1 % sequence similarity, which was followed by Streptomyces durhamensis NRRL B-3309T (99.0 %), Streptomyces filipinensis NBRC 12860T (98.9 %) and Streptomyces yaanensis Z4T (98.7 %).
30896386	6	25	theme	Streptomyces	898:909	arg1	%					931:931	98.7 %	926:931	98.7 %	926:931	Phylogenetic analysis based on its 16S rRNA gene sequence indicated that the novel strain should be assigned to the genus Streptomyces, and the closest species was Streptomyces puniciscabiei S77T with 99.1 % sequence similarity, which was followed by Streptomyces durhamensis NRRL B-3309T (99.0 %), Streptomyces filipinensis NBRC 12860T (98.9 %) and Streptomyces yaanensis Z4T (98.7 %).
30896386	6	26	with	S77T	739:742	arg1	similarity					765:774	99.1 % sequence similarity	749:774	99.1 % sequence similarity	749:774	Phylogenetic analysis based on its 16S rRNA gene sequence indicated that the novel strain should be assigned to the genus Streptomyces, and the closest species was Streptomyces puniciscabiei S77T with 99.1 % sequence similarity, which was followed by Streptomyces durhamensis NRRL B-3309T (99.0 %), Streptomyces filipinensis NBRC 12860T (98.9 %) and Streptomyces yaanensis Z4T (98.7 %).
30896386	6	27	theme	Phylogenetic	548:559	arg1	analysis					561:568	Phylogenetic analysis	548:568	Phylogenetic analysis based on its 16S rRNA gene sequence	548:604	Phylogenetic analysis based on its 16S rRNA gene sequence indicated that the novel strain should be assigned to the genus Streptomyces, and the closest species was Streptomyces puniciscabiei S77T with 99.1 % sequence similarity, which was followed by Streptomyces durhamensis NRRL B-3309T (99.0 %), Streptomyces filipinensis NBRC 12860T (98.9 %) and Streptomyces yaanensis Z4T (98.7 %).
30896386	10	28	theme	polar	1294:1298	arg1	phosphatidylglycerol					1312:1331	phosphatidylglycerol	1312:1331	phosphatidylglycerol	1312:1331	The predominant isoprenoid quinones were MK-9(H4) and MK-9(H6), the major polar lipids were phosphatidylglycerol and an unidentified phospholipid, and the main fatty acids were iso-C16 : 0 and anteiso-C15 : 0.
30896386	10	28	theme	polar	1294:1298	arg1	lipids					1300:1305	the major polar lipids	1284:1305	the major polar lipids	1284:1305	The predominant isoprenoid quinones were MK-9(H4) and MK-9(H6), the major polar lipids were phosphatidylglycerol and an unidentified phospholipid, and the main fatty acids were iso-C16 : 0 and anteiso-C15 : 0.
30896386	13	29	theme	genomic	1734:1740	arg1	evidence					1742:1749	The phylogenetic, phenotypic and chemotaxonomic as well as genomic evidence	1675:1749	evidence	1742:1749	The phylogenetic, phenotypic and chemotaxonomic as well as genomic evidence supported that TW1S1T represents a novel species of Streptomyces, for which the name Streptomycesfodineus sp.
30896386	1	30	theme	antifungal	56:65	arg1	activity					67:74	antifungal activity	56:74	antifungal activity	56:74	nov., an actinobacterium with antifungal activity isolated from mine area soil.
30896386	3	31	theme	aerial	321:326	arg1	mycelium					328:335	white-grey aerial mycelium	310:335	white-grey aerial mycelium	310:335	The novel strain, designated TW1S1T, formed white-grey aerial mycelium and yellow substrate mycelium on oatmeal agar.
30896386	13	32	theme	chemotaxonomic	1708:1721	arg1	evidence					1742:1749	The phylogenetic, phenotypic and chemotaxonomic as well as genomic evidence	1675:1749	evidence	1742:1749	The phylogenetic, phenotypic and chemotaxonomic as well as genomic evidence supported that TW1S1T represents a novel species of Streptomyces, for which the name Streptomycesfodineus sp.
30896386	4	33	dep	8 	475:476	arg1	to					472:473	to	472:473	to	472:473	Growth occurred at 10-45 °C (optimum, 30 °C), pH 4-9 (pH 6-7) and in the presence of up to 8 % (w/v) NaCl.
30896386	6	34	dep	Streptomyces	898:909	arg1	yaanensis					911:919	yaanensis	911:919	yaanensis	911:919	Phylogenetic analysis based on its 16S rRNA gene sequence indicated that the novel strain should be assigned to the genus Streptomyces, and the closest species was Streptomyces puniciscabiei S77T with 99.1 % sequence similarity, which was followed by Streptomyces durhamensis NRRL B-3309T (99.0 %), Streptomyces filipinensis NBRC 12860T (98.9 %) and Streptomyces yaanensis Z4T (98.7 %).
30896386	0	35	theme	Streptomyces	0:11	arg1	sp					22:23	Streptomyces fodineus sp	0:23	Streptomyces fodineus sp.	0:24	Streptomyces fodineus sp.
30896386	1	36	theme	mine	90:93	arg1	soil					100:103	mine area soil	90:103	mine area soil	90:103	nov., an actinobacterium with antifungal activity isolated from mine area soil.
30896386	3	37	theme	substrate	348:356	arg1	mycelium					358:365	yellow substrate mycelium	341:365	yellow substrate mycelium	341:365	The novel strain, designated TW1S1T, formed white-grey aerial mycelium and yellow substrate mycelium on oatmeal agar.
30896386	14	38	theme	=	1913:1913	arg1	32404T					1919:1924	TW1S1T = KCTC 49013T = JCM 32404T	1892:1924	TW1S1T = KCTC 49013T = JCM 32404T	1892:1924	nov. is proposed (type strain, TW1S1T = KCTC 49013T = JCM 32404T).
30896386	6	39	theme	rRNA	587:590	arg1	sequence					597:604	its 16S rRNA gene sequence	579:604	its 16S rRNA gene sequence	579:604	Phylogenetic analysis based on its 16S rRNA gene sequence indicated that the novel strain should be assigned to the genus Streptomyces, and the closest species was Streptomyces puniciscabiei S77T with 99.1 % sequence similarity, which was followed by Streptomyces durhamensis NRRL B-3309T (99.0 %), Streptomyces filipinensis NBRC 12860T (98.9 %) and Streptomyces yaanensis Z4T (98.7 %).
30896386	10	40	theme	unidentified	1340:1351	arg1	phospholipid					1353:1364	an unidentified phospholipid	1337:1364	an unidentified phospholipid	1337:1364	The predominant isoprenoid quinones were MK-9(H4) and MK-9(H6), the major polar lipids were phosphatidylglycerol and an unidentified phospholipid, and the main fatty acids were iso-C16 : 0 and anteiso-C15 : 0.
30896386	13	41	theme	phenotypic	1693:1702	arg1	evidence					1742:1749	The phylogenetic, phenotypic and chemotaxonomic as well as genomic evidence	1675:1749	evidence	1742:1749	The phylogenetic, phenotypic and chemotaxonomic as well as genomic evidence supported that TW1S1T represents a novel species of Streptomyces, for which the name Streptomycesfodineus sp.
30896386	6	42	theme	sequence	756:763	arg1	similarity					765:774	99.1 % sequence similarity	749:774	99.1 % sequence similarity	749:774	Phylogenetic analysis based on its 16S rRNA gene sequence indicated that the novel strain should be assigned to the genus Streptomyces, and the closest species was Streptomyces puniciscabiei S77T with 99.1 % sequence similarity, which was followed by Streptomyces durhamensis NRRL B-3309T (99.0 %), Streptomyces filipinensis NBRC 12860T (98.9 %) and Streptomyces yaanensis Z4T (98.7 %).
30896386	12	43	theme	separate	1657:1664	arg1	species					1666:1672	a separate species	1655:1672	a separate species	1655:1672	In addition, the genome-based comparison with the closest species indicated that strain TW1S1T should be recognized as a separate species.
30896386	12	43	theme	separate	1657:1664	arg1	TW1S1T					1624:1629	strain TW1S1T	1617:1629	strain TW1S1T	1617:1629	In addition, the genome-based comparison with the closest species indicated that strain TW1S1T should be recognized as a separate species.
30896386	10	44	theme	fatty	1380:1384	arg1	iso-C16 					1397:1404	iso-C16 	1397:1404	iso-C16 	1397:1404	The predominant isoprenoid quinones were MK-9(H4) and MK-9(H6), the major polar lipids were phosphatidylglycerol and an unidentified phospholipid, and the main fatty acids were iso-C16 : 0 and anteiso-C15 : 0.
30896386	10	44	theme	fatty	1380:1384	arg1	acids					1386:1390	the main fatty acids	1371:1390	the main fatty acids	1371:1390	The predominant isoprenoid quinones were MK-9(H4) and MK-9(H6), the major polar lipids were phosphatidylglycerol and an unidentified phospholipid, and the main fatty acids were iso-C16 : 0 and anteiso-C15 : 0.
30896386	6	45	theme	99.1 	749:753	arg1	%					754:754	%	754:754	%	754:754	Phylogenetic analysis based on its 16S rRNA gene sequence indicated that the novel strain should be assigned to the genus Streptomyces, and the closest species was Streptomyces puniciscabiei S77T with 99.1 % sequence similarity, which was followed by Streptomyces durhamensis NRRL B-3309T (99.0 %), Streptomyces filipinensis NBRC 12860T (98.9 %) and Streptomyces yaanensis Z4T (98.7 %).
30896386	10	46	theme	predominant	1224:1234	arg1	quinones					1247:1254	The predominant isoprenoid quinones	1220:1254	The predominant isoprenoid quinones	1220:1254	The predominant isoprenoid quinones were MK-9(H4) and MK-9(H6), the major polar lipids were phosphatidylglycerol and an unidentified phospholipid, and the main fatty acids were iso-C16 : 0 and anteiso-C15 : 0.
30896386	10	46	theme	predominant	1224:1234	arg1	MK-9					1261:1264	MK-9	1261:1264	MK-9	1261:1264	The predominant isoprenoid quinones were MK-9(H4) and MK-9(H6), the major polar lipids were phosphatidylglycerol and an unidentified phospholipid, and the main fatty acids were iso-C16 : 0 and anteiso-C15 : 0.
30896386	13	47	theme	Streptomycesfodineus	1836:1855	arg1	sp					1857:1858	the name Streptomycesfodineus sp	1827:1858	the name Streptomycesfodineus sp	1827:1858	The phylogenetic, phenotypic and chemotaxonomic as well as genomic evidence supported that TW1S1T represents a novel species of Streptomyces, for which the name Streptomycesfodineus sp.
30896386	4	48	dep	10-45 °C	403:410	arg1	30 °C					422:426	30 °C	422:426	30 °C	422:426	Growth occurred at 10-45 °C (optimum, 30 °C), pH 4-9 (pH 6-7) and in the presence of up to 8 % (w/v) NaCl.
30896386	4	48	dep	10-45 °C	403:410	arg1	optimum					413:419	optimum	413:419	optimum	413:419	Growth occurred at 10-45 °C (optimum, 30 °C), pH 4-9 (pH 6-7) and in the presence of up to 8 % (w/v) NaCl.
30896386	1	49	with	actinobacterium	35:49	arg1	activity					67:74	antifungal activity	56:74	antifungal activity	56:74	nov., an actinobacterium with antifungal activity isolated from mine area soil.
30896386	2	50	theme	novel	108:112	arg1	strain					130:135	A novel actinobacterial strain	106:135	A novel actinobacterial strain producing an antifungal substance	106:169	A novel actinobacterial strain producing an antifungal substance was isolated from a sample of acidic mine area soil, and its taxonomic position was evaluated.
30896386	8	51	attach	present	1109:1115	arg2	glycine					1096:1102	glycine	1096:1102	glycine	1096:1102	ll-Diaminopimelic acid was the diagnostic diamino acid, and alanine, glutamic acid and glycine were present in the peptidoglycan.
30896386	8	51	attach	present	1109:1115	arg1	peptidoglycan					1124:1136	the peptidoglycan	1120:1136	the peptidoglycan	1120:1136	ll-Diaminopimelic acid was the diagnostic diamino acid, and alanine, glutamic acid and glycine were present in the peptidoglycan.
30896386	8	51	attach	present	1109:1115	arg2	acid					1087:1090	glutamic acid	1078:1090	glutamic acid	1078:1090	ll-Diaminopimelic acid was the diagnostic diamino acid, and alanine, glutamic acid and glycine were present in the peptidoglycan.
30896386	8	51	attach	present	1109:1115	arg2	alanine					1069:1075	alanine	1069:1075	alanine	1069:1075	ll-Diaminopimelic acid was the diagnostic diamino acid, and alanine, glutamic acid and glycine were present in the peptidoglycan.
30896386	6	52	theme	novel	625:629	arg1	strain					631:636	the novel strain	621:636	the novel strain	621:636	Phylogenetic analysis based on its 16S rRNA gene sequence indicated that the novel strain should be assigned to the genus Streptomyces, and the closest species was Streptomyces puniciscabiei S77T with 99.1 % sequence similarity, which was followed by Streptomyces durhamensis NRRL B-3309T (99.0 %), Streptomyces filipinensis NBRC 12860T (98.9 %) and Streptomyces yaanensis Z4T (98.7 %).
30896386	14	53	theme	TW1S1T	1892:1897	arg1	32404T					1919:1924	TW1S1T = KCTC 49013T = JCM 32404T	1892:1924	TW1S1T = KCTC 49013T = JCM 32404T	1892:1924	nov. is proposed (type strain, TW1S1T = KCTC 49013T = JCM 32404T).
30896386	6	54	dep	Streptomyces	799:810	arg1	durhamensis					812:822	durhamensis	812:822	durhamensis	812:822	Phylogenetic analysis based on its 16S rRNA gene sequence indicated that the novel strain should be assigned to the genus Streptomyces, and the closest species was Streptomyces puniciscabiei S77T with 99.1 % sequence similarity, which was followed by Streptomyces durhamensis NRRL B-3309T (99.0 %), Streptomyces filipinensis NBRC 12860T (98.9 %) and Streptomyces yaanensis Z4T (98.7 %).
30896386	8	55	theme	diagnostic	1040:1049	arg1	acid					1059:1062	the diagnostic diamino acid	1036:1062	the diagnostic diamino acid	1036:1062	ll-Diaminopimelic acid was the diagnostic diamino acid, and alanine, glutamic acid and glycine were present in the peptidoglycan.
30896386	8	55	theme	diagnostic	1040:1049	arg1	acid					1027:1030	ll-Diaminopimelic acid	1009:1030	ll-Diaminopimelic acid	1009:1030	ll-Diaminopimelic acid was the diagnostic diamino acid, and alanine, glutamic acid and glycine were present in the peptidoglycan.
30896386	4	56	theme	NaCl	485:488	arg1	presence					457:464	the presence	453:464	the presence of up to 8 % (w/v) NaCl	453:488	Growth occurred at 10-45 °C (optimum, 30 °C), pH 4-9 (pH 6-7) and in the presence of up to 8 % (w/v) NaCl.
30896386	11	57	theme	phenotypic	1513:1522	arg1	properties					1524:1533	its phenotypic properties	1509:1533	its phenotypic properties	1509:1533	However, strain TW1S1T could be distinguished from its neighbouring species by its phenotypic properties.
30896386	5	58	theme	peptone-yeast	515:527	arg1	agar					542:545	peptone-yeast extract-iron agar	515:545	peptone-yeast extract-iron agar	515:545	Melanin was produced on peptone-yeast extract-iron agar.
30896386	6	59	theme	closest	692:698	arg1	species					700:706	the closest species	688:706	the closest species	688:706	Phylogenetic analysis based on its 16S rRNA gene sequence indicated that the novel strain should be assigned to the genus Streptomyces, and the closest species was Streptomyces puniciscabiei S77T with 99.1 % sequence similarity, which was followed by Streptomyces durhamensis NRRL B-3309T (99.0 %), Streptomyces filipinensis NBRC 12860T (98.9 %) and Streptomyces yaanensis Z4T (98.7 %).
30896386	6	59	theme	closest	692:698	arg1	S77T					739:742	Streptomyces puniciscabiei S77T	712:742	Streptomyces puniciscabiei S77T	712:742	Phylogenetic analysis based on its 16S rRNA gene sequence indicated that the novel strain should be assigned to the genus Streptomyces, and the closest species was Streptomyces puniciscabiei S77T with 99.1 % sequence similarity, which was followed by Streptomyces durhamensis NRRL B-3309T (99.0 %), Streptomyces filipinensis NBRC 12860T (98.9 %) and Streptomyces yaanensis Z4T (98.7 %).
30896386	6	60	theme	NBRC	873:876	arg1	12860T					878:883	Streptomyces filipinensis NBRC 12860T	847:883	Streptomyces filipinensis NBRC 12860T (98.9 %)	847:892	Phylogenetic analysis based on its 16S rRNA gene sequence indicated that the novel strain should be assigned to the genus Streptomyces, and the closest species was Streptomyces puniciscabiei S77T with 99.1 % sequence similarity, which was followed by Streptomyces durhamensis NRRL B-3309T (99.0 %), Streptomyces filipinensis NBRC 12860T (98.9 %) and Streptomyces yaanensis Z4T (98.7 %).
30896386	6	60	theme	NBRC	873:876	arg1	%					891:891	98.9 %	886:891	98.9 %	886:891	Phylogenetic analysis based on its 16S rRNA gene sequence indicated that the novel strain should be assigned to the genus Streptomyces, and the closest species was Streptomyces puniciscabiei S77T with 99.1 % sequence similarity, which was followed by Streptomyces durhamensis NRRL B-3309T (99.0 %), Streptomyces filipinensis NBRC 12860T (98.9 %) and Streptomyces yaanensis Z4T (98.7 %).
30896386	13	61	theme	Streptomyces	1803:1814	arg1	species					1792:1798	a novel species	1784:1798	a novel species	1784:1798	The phylogenetic, phenotypic and chemotaxonomic as well as genomic evidence supported that TW1S1T represents a novel species of Streptomyces, for which the name Streptomycesfodineus sp.
30896386	6	62	theme	Streptomyces	847:858	arg1	12860T					878:883	Streptomyces filipinensis NBRC 12860T	847:883	Streptomyces filipinensis NBRC 12860T (98.9 %)	847:892	Phylogenetic analysis based on its 16S rRNA gene sequence indicated that the novel strain should be assigned to the genus Streptomyces, and the closest species was Streptomyces puniciscabiei S77T with 99.1 % sequence similarity, which was followed by Streptomyces durhamensis NRRL B-3309T (99.0 %), Streptomyces filipinensis NBRC 12860T (98.9 %) and Streptomyces yaanensis Z4T (98.7 %).
30896386	6	62	theme	Streptomyces	847:858	arg1	%					891:891	98.9 %	886:891	98.9 %	886:891	Phylogenetic analysis based on its 16S rRNA gene sequence indicated that the novel strain should be assigned to the genus Streptomyces, and the closest species was Streptomyces puniciscabiei S77T with 99.1 % sequence similarity, which was followed by Streptomyces durhamensis NRRL B-3309T (99.0 %), Streptomyces filipinensis NBRC 12860T (98.9 %) and Streptomyces yaanensis Z4T (98.7 %).
30896386	6	63	dep	Streptomyces	712:723	arg1	puniciscabiei					725:737	puniciscabiei	725:737	puniciscabiei	725:737	Phylogenetic analysis based on its 16S rRNA gene sequence indicated that the novel strain should be assigned to the genus Streptomyces, and the closest species was Streptomyces puniciscabiei S77T with 99.1 % sequence similarity, which was followed by Streptomyces durhamensis NRRL B-3309T (99.0 %), Streptomyces filipinensis NBRC 12860T (98.9 %) and Streptomyces yaanensis Z4T (98.7 %).
30896386	4	64	dep	NaCl	485:488	arg1	%					477:477	%	477:477	%	477:477	Growth occurred at 10-45 °C (optimum, 30 °C), pH 4-9 (pH 6-7) and in the presence of up to 8 % (w/v) NaCl.
30896386	2	65	theme	area	213:216	arg1	soil					218:221	acidic mine area soil	201:221	acidic mine area soil	201:221	A novel actinobacterial strain producing an antifungal substance was isolated from a sample of acidic mine area soil, and its taxonomic position was evaluated.
30896386	6	66	dep	Streptomyces	847:858	arg1	filipinensis					860:871	filipinensis	860:871	filipinensis	860:871	Phylogenetic analysis based on its 16S rRNA gene sequence indicated that the novel strain should be assigned to the genus Streptomyces, and the closest species was Streptomyces puniciscabiei S77T with 99.1 % sequence similarity, which was followed by Streptomyces durhamensis NRRL B-3309T (99.0 %), Streptomyces filipinensis NBRC 12860T (98.9 %) and Streptomyces yaanensis Z4T (98.7 %).
30896386	8	67	from	present	1109:1115	arg1	peptidoglycan					1124:1136	the peptidoglycan	1120:1136	the peptidoglycan	1120:1136	ll-Diaminopimelic acid was the diagnostic diamino acid, and alanine, glutamic acid and glycine were present in the peptidoglycan.
30896386	12	68	theme	genome-based	1553:1564	arg1	comparison					1566:1575	the genome-based comparison	1549:1575	the genome-based comparison with the closest species	1549:1600	In addition, the genome-based comparison with the closest species indicated that strain TW1S1T should be recognized as a separate species.
30896386	3	69	theme	white-grey	310:319	arg1	mycelium					328:335	white-grey aerial mycelium	310:335	white-grey aerial mycelium	310:335	The novel strain, designated TW1S1T, formed white-grey aerial mycelium and yellow substrate mycelium on oatmeal agar.
30896386	8	70	located	present	1109:1115	arg2	glycine					1096:1102	glycine	1096:1102	glycine	1096:1102	ll-Diaminopimelic acid was the diagnostic diamino acid, and alanine, glutamic acid and glycine were present in the peptidoglycan.
30896386	8	70	located	present	1109:1115	arg1	peptidoglycan					1124:1136	the peptidoglycan	1120:1136	the peptidoglycan	1120:1136	ll-Diaminopimelic acid was the diagnostic diamino acid, and alanine, glutamic acid and glycine were present in the peptidoglycan.
30896386	8	70	located	present	1109:1115	arg2	acid					1087:1090	glutamic acid	1078:1090	glutamic acid	1078:1090	ll-Diaminopimelic acid was the diagnostic diamino acid, and alanine, glutamic acid and glycine were present in the peptidoglycan.
30896386	8	70	located	present	1109:1115	arg2	alanine					1069:1075	alanine	1069:1075	alanine	1069:1075	ll-Diaminopimelic acid was the diagnostic diamino acid, and alanine, glutamic acid and glycine were present in the peptidoglycan.
30896386	10	71	theme	major	1288:1292	arg1	phosphatidylglycerol					1312:1331	phosphatidylglycerol	1312:1331	phosphatidylglycerol	1312:1331	The predominant isoprenoid quinones were MK-9(H4) and MK-9(H6), the major polar lipids were phosphatidylglycerol and an unidentified phospholipid, and the main fatty acids were iso-C16 : 0 and anteiso-C15 : 0.
30896386	10	71	theme	major	1288:1292	arg1	lipids					1300:1305	the major polar lipids	1284:1305	the major polar lipids	1284:1305	The predominant isoprenoid quinones were MK-9(H4) and MK-9(H6), the major polar lipids were phosphatidylglycerol and an unidentified phospholipid, and the main fatty acids were iso-C16 : 0 and anteiso-C15 : 0.
30896386	12	72	with	comparison	1566:1575	arg1	species					1594:1600	the closest species	1582:1600	the closest species	1582:1600	In addition, the genome-based comparison with the closest species indicated that strain TW1S1T should be recognized as a separate species.
30896386	3	73	theme	yellow	341:346	arg1	mycelium					358:365	yellow substrate mycelium	341:365	yellow substrate mycelium	341:365	The novel strain, designated TW1S1T, formed white-grey aerial mycelium and yellow substrate mycelium on oatmeal agar.
30896386	4	74	dep	%	477:477	arg1	8 					475:476	8 	475:476	8 	475:476	Growth occurred at 10-45 °C (optimum, 30 °C), pH 4-9 (pH 6-7) and in the presence of up to 8 % (w/v) NaCl.
30896386	4	74	dep	%	477:477	arg1	w/v					480:482	w/v	480:482	w/v	480:482	Growth occurred at 10-45 °C (optimum, 30 °C), pH 4-9 (pH 6-7) and in the presence of up to 8 % (w/v) NaCl.
30896386	9	75	contain	contained	1170:1178	arg2	mannose					1200:1206	mannose	1200:1206	mannose	1200:1206	The cell-wall hydrolysate also contained galactose, glucose, mannose and ribose.
30896386	9	75	contain	contained	1170:1178	arg2	ribose					1212:1217	ribose	1212:1217	ribose	1212:1217	The cell-wall hydrolysate also contained galactose, glucose, mannose and ribose.
30896386	9	75	contain	contained	1170:1178	arg2	galactose					1180:1188	galactose	1180:1188	galactose	1180:1188	The cell-wall hydrolysate also contained galactose, glucose, mannose and ribose.
30896386	9	75	contain	contained	1170:1178	arg2	glucose					1191:1197	glucose	1191:1197	glucose	1191:1197	The cell-wall hydrolysate also contained galactose, glucose, mannose and ribose.
30896386	9	75	contain	contained	1170:1178	arg1	hydrolysate					1153:1163	The cell-wall hydrolysate	1139:1163	The cell-wall hydrolysate	1139:1163	The cell-wall hydrolysate also contained galactose, glucose, mannose and ribose.
30896386	0	76	theme	fodineus	13:20	arg1	sp					22:23	Streptomyces fodineus sp	0:23	Streptomyces fodineus sp.	0:24	Streptomyces fodineus sp.
30896386	3	77	theme	oatmeal	370:376	arg1	agar					378:381	oatmeal agar	370:381	oatmeal agar	370:381	The novel strain, designated TW1S1T, formed white-grey aerial mycelium and yellow substrate mycelium on oatmeal agar.
30896386	14	78	theme	JCM	1915:1917	arg1	32404T					1919:1924	TW1S1T = KCTC 49013T = JCM 32404T	1892:1924	TW1S1T = KCTC 49013T = JCM 32404T	1892:1924	nov. is proposed (type strain, TW1S1T = KCTC 49013T = JCM 32404T).
30896386	6	79	theme	gene	592:595	arg1	sequence					597:604	its 16S rRNA gene sequence	579:604	its 16S rRNA gene sequence	579:604	Phylogenetic analysis based on its 16S rRNA gene sequence indicated that the novel strain should be assigned to the genus Streptomyces, and the closest species was Streptomyces puniciscabiei S77T with 99.1 % sequence similarity, which was followed by Streptomyces durhamensis NRRL B-3309T (99.0 %), Streptomyces filipinensis NBRC 12860T (98.9 %) and Streptomyces yaanensis Z4T (98.7 %).
30896386	1	80	theme	area	95:98	arg1	soil					100:103	mine area soil	90:103	mine area soil	90:103	nov., an actinobacterium with antifungal activity isolated from mine area soil.
30896386	2	81	attach	isolated	175:182	arg1	sample					191:196	a sample	189:196	a sample of acidic mine area soil	189:221	A novel actinobacterial strain producing an antifungal substance was isolated from a sample of acidic mine area soil, and its taxonomic position was evaluated.
30896386	2	81	attach	isolated	175:182	arg2	strain					130:135	A novel actinobacterial strain	106:135	A novel actinobacterial strain producing an antifungal substance	106:169	A novel actinobacterial strain producing an antifungal substance was isolated from a sample of acidic mine area soil, and its taxonomic position was evaluated.
30896386	14	82	theme	49013T	1906:1911	arg1	32404T					1919:1924	TW1S1T = KCTC 49013T = JCM 32404T	1892:1924	TW1S1T = KCTC 49013T = JCM 32404T	1892:1924	nov. is proposed (type strain, TW1S1T = KCTC 49013T = JCM 32404T).
30896386	6	83	theme	16S	583:585	arg1	sequence					597:604	its 16S rRNA gene sequence	579:604	its 16S rRNA gene sequence	579:604	Phylogenetic analysis based on its 16S rRNA gene sequence indicated that the novel strain should be assigned to the genus Streptomyces, and the closest species was Streptomyces puniciscabiei S77T with 99.1 % sequence similarity, which was followed by Streptomyces durhamensis NRRL B-3309T (99.0 %), Streptomyces filipinensis NBRC 12860T (98.9 %) and Streptomyces yaanensis Z4T (98.7 %).
30896386	14	84	theme	=	1899:1899	arg1	32404T					1919:1924	TW1S1T = KCTC 49013T = JCM 32404T	1892:1924	TW1S1T = KCTC 49013T = JCM 32404T	1892:1924	nov. is proposed (type strain, TW1S1T = KCTC 49013T = JCM 32404T).
30896386	6	85	theme	genus	664:668	arg1	Streptomyces					670:681	the genus Streptomyces	660:681	the genus Streptomyces	660:681	Phylogenetic analysis based on its 16S rRNA gene sequence indicated that the novel strain should be assigned to the genus Streptomyces, and the closest species was Streptomyces puniciscabiei S77T with 99.1 % sequence similarity, which was followed by Streptomyces durhamensis NRRL B-3309T (99.0 %), Streptomyces filipinensis NBRC 12860T (98.9 %) and Streptomyces yaanensis Z4T (98.7 %).
30896386	6	86	theme	%	754:754	arg1	similarity					765:774	99.1 % sequence similarity	749:774	99.1 % sequence similarity	749:774	Phylogenetic analysis based on its 16S rRNA gene sequence indicated that the novel strain should be assigned to the genus Streptomyces, and the closest species was Streptomyces puniciscabiei S77T with 99.1 % sequence similarity, which was followed by Streptomyces durhamensis NRRL B-3309T (99.0 %), Streptomyces filipinensis NBRC 12860T (98.9 %) and Streptomyces yaanensis Z4T (98.7 %).
30896386	10	87	theme	main	1375:1378	arg1	iso-C16 					1397:1404	iso-C16 	1397:1404	iso-C16 	1397:1404	The predominant isoprenoid quinones were MK-9(H4) and MK-9(H6), the major polar lipids were phosphatidylglycerol and an unidentified phospholipid, and the main fatty acids were iso-C16 : 0 and anteiso-C15 : 0.
30896386	10	87	theme	main	1375:1378	arg1	acids					1386:1390	the main fatty acids	1371:1390	the main fatty acids	1371:1390	The predominant isoprenoid quinones were MK-9(H4) and MK-9(H6), the major polar lipids were phosphatidylglycerol and an unidentified phospholipid, and the main fatty acids were iso-C16 : 0 and anteiso-C15 : 0.
30896386	2	88	theme	acidic	201:206	arg1	soil					218:221	acidic mine area soil	201:221	acidic mine area soil	201:221	A novel actinobacterial strain producing an antifungal substance was isolated from a sample of acidic mine area soil, and its taxonomic position was evaluated.
30896386	7	89	with	consistent	970:979	arg1	those					986:990	those	986:990	those	986:990	The chemotaxonomic properties were consistent with those of Streptomyces.
30896386	14	90	theme	type	1879:1882	arg1	strain					1884:1889	type strain	1879:1889	type strain	1879:1889	nov. is proposed (type strain, TW1S1T = KCTC 49013T = JCM 32404T).
30896386	13	91	theme	name	1831:1834	arg1	sp					1857:1858	the name Streptomycesfodineus sp	1827:1858	the name Streptomycesfodineus sp	1827:1858	The phylogenetic, phenotypic and chemotaxonomic as well as genomic evidence supported that TW1S1T represents a novel species of Streptomyces, for which the name Streptomycesfodineus sp.
30896386	2	92	theme	actinobacterial	114:128	arg1	strain					130:135	A novel actinobacterial strain	106:135	A novel actinobacterial strain producing an antifungal substance	106:169	A novel actinobacterial strain producing an antifungal substance was isolated from a sample of acidic mine area soil, and its taxonomic position was evaluated.
30896386	11	93	theme	strain	1439:1444	arg1	TW1S1T					1446:1451	strain TW1S1T	1439:1451	strain TW1S1T	1439:1451	However, strain TW1S1T could be distinguished from its neighbouring species by its phenotypic properties.
29620494	1	0	with	nov.	21:24	arg1	ability					36:42	the ability to degrade petroleum oil	32:67	the ability to degrade petroleum oil	32:67	nov., with the ability to degrade petroleum oil, isolated from oil-contaminated soil.
29620494	10	1	theme	genus	1464:1468	arg1	Rhodococcus					1470:1480	the genus Rhodococcus	1460:1480	the genus Rhodococcus	1460:1480	The DNA-DNA relatedness values between strain Ktm-20T and closely related species of the genus Rhodococcus were between 38.3-25.3 %, which falls below the threshold value of 70 % for the strain to be considered as novel.
29620494	6	2	theme	respiratory	858:868	arg1	quinone					870:876	The only respiratory quinone	849:876	The only respiratory quinone	849:876	The only respiratory quinone was MK-8(H2); the major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside; and the predominant fatty acids were C16 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and C18 : 1ω9c.
29620494	6	2	theme	respiratory	858:868	arg1	MK-8					882:885	MK-8	882:885	MK-8	882:885	The only respiratory quinone was MK-8(H2); the major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside; and the predominant fatty acids were C16 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and C18 : 1ω9c.
29620494	2	3	theme	oil-contaminated	274:289	arg1	soil					291:294	oil-contaminated soil	274:294	oil-contaminated soil	274:294	A Gram-stain-positive, non-motile, creamy-white and rod-coccus shaped actinobacterium, designated strain Ktm-20T, capable of degrading petroleum oil was isolated from oil-contaminated soil.
29620494	9	4	theme	70.9	1364:1367	arg1	mol					1369:1371	mol	1369:1371	mol	1369:1371	The DNA G+C content was 70.9 mol%.
29620494	5	5	theme	Rhodococcus	593:603	arg1	44892T					619:624	Rhodococcus triatomae DSM 44892T	593:624	Rhodococcus triatomae DSM 44892T	593:624	The 16S rRNA gene sequence analysis showed that strain Ktm-20T belonged to the genus Rhodococcus and is closely related to Rhodococcus triatomae DSM 44892T, Rhodococcus pedocola UC12T, Rhodococcus wratislaviensis NBRC 100605T, Rhodococcus agglutinans CFH S0262T and Rhodococcus canchipurensis MBRL 353T (98.8, 98.7, 98.5, 98.4 and 98.3 % gene sequence similarity, respectively).
29620494	5	6	theme	16S	474:476	arg1	analysis					497:504	The 16S rRNA gene sequence analysis	470:504	The 16S rRNA gene sequence analysis	470:504	The 16S rRNA gene sequence analysis showed that strain Ktm-20T belonged to the genus Rhodococcus and is closely related to Rhodococcus triatomae DSM 44892T, Rhodococcus pedocola UC12T, Rhodococcus wratislaviensis NBRC 100605T, Rhodococcus agglutinans CFH S0262T and Rhodococcus canchipurensis MBRL 353T (98.8, 98.7, 98.5, 98.4 and 98.3 % gene sequence similarity, respectively).
29620494	5	7	theme	DSM	615:617	arg1	44892T					619:624	Rhodococcus triatomae DSM 44892T	593:624	Rhodococcus triatomae DSM 44892T	593:624	The 16S rRNA gene sequence analysis showed that strain Ktm-20T belonged to the genus Rhodococcus and is closely related to Rhodococcus triatomae DSM 44892T, Rhodococcus pedocola UC12T, Rhodococcus wratislaviensis NBRC 100605T, Rhodococcus agglutinans CFH S0262T and Rhodococcus canchipurensis MBRL 353T (98.8, 98.7, 98.5, 98.4 and 98.3 % gene sequence similarity, respectively).
29620494	6	8	theme	polar	902:906	arg1	phosphatidylethanolamine					920:943	phosphatidylethanolamine	920:943	phosphatidylethanolamine	920:943	The only respiratory quinone was MK-8(H2); the major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside; and the predominant fatty acids were C16 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and C18 : 1ω9c.
29620494	6	8	theme	polar	902:906	arg1	lipids					908:913	the major polar lipids	892:913	the major polar lipids	892:913	The only respiratory quinone was MK-8(H2); the major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside; and the predominant fatty acids were C16 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and C18 : 1ω9c.
29620494	11	9	theme	closest	1714:1720	arg1	neighbours					1735:1744	its closest phylogenetic neighbours	1710:1744	its closest phylogenetic neighbours	1710:1744	The morphological, physiological, chemotaxonomic and phylogenetic analyses clearly distinguished this strain from its closest phylogenetic neighbours.
29620494	5	10	dep	Rhodococcus	736:746	arg1	canchipurensis					748:761	canchipurensis	748:761	canchipurensis	748:761	The 16S rRNA gene sequence analysis showed that strain Ktm-20T belonged to the genus Rhodococcus and is closely related to Rhodococcus triatomae DSM 44892T, Rhodococcus pedocola UC12T, Rhodococcus wratislaviensis NBRC 100605T, Rhodococcus agglutinans CFH S0262T and Rhodococcus canchipurensis MBRL 353T (98.8, 98.7, 98.5, 98.4 and 98.3 % gene sequence similarity, respectively).
29620494	5	11	theme	Rhodococcus	697:707	arg1	S0262T					725:730	Rhodococcus agglutinans CFH S0262T	697:730	Rhodococcus agglutinans CFH S0262T	697:730	The 16S rRNA gene sequence analysis showed that strain Ktm-20T belonged to the genus Rhodococcus and is closely related to Rhodococcus triatomae DSM 44892T, Rhodococcus pedocola UC12T, Rhodococcus wratislaviensis NBRC 100605T, Rhodococcus agglutinans CFH S0262T and Rhodococcus canchipurensis MBRL 353T (98.8, 98.7, 98.5, 98.4 and 98.3 % gene sequence similarity, respectively).
29620494	2	12	theme	capable	221:227	arg1	actinobacterium					177:191	A Gram-stain-positive, non-motile, creamy-white and rod-coccus shaped actinobacterium	107:191	A Gram-stain-positive, non-motile, creamy-white and rod-coccus shaped actinobacterium	107:191	A Gram-stain-positive, non-motile, creamy-white and rod-coccus shaped actinobacterium, designated strain Ktm-20T, capable of degrading petroleum oil was isolated from oil-contaminated soil.
29620494	5	13	theme	gene	808:811	arg1	similarity					822:831	98.8, 98.7, 98.5, 98.4 and 98.3 % gene sequence similarity	774:831	98.8, 98.7, 98.5, 98.4 and 98.3 % gene sequence similarity	774:831	The 16S rRNA gene sequence analysis showed that strain Ktm-20T belonged to the genus Rhodococcus and is closely related to Rhodococcus triatomae DSM 44892T, Rhodococcus pedocola UC12T, Rhodococcus wratislaviensis NBRC 100605T, Rhodococcus agglutinans CFH S0262T and Rhodococcus canchipurensis MBRL 353T (98.8, 98.7, 98.5, 98.4 and 98.3 % gene sequence similarity, respectively).
29620494	7	14	theme	whole-cell	1286:1295	arg1	hydrolysates					1297:1308	whole-cell hydrolysates	1286:1308	whole-cell hydrolysates	1286:1308	The cell-wall peptidoglycan contained meso-diaminopimelic acid; and galactose, glucose, arabinose and ribose were detected as diagnostic sugars from whole-cell hydrolysates.
29620494	5	15	theme	gene	483:486	arg1	analysis					497:504	The 16S rRNA gene sequence analysis	470:504	The 16S rRNA gene sequence analysis	470:504	The 16S rRNA gene sequence analysis showed that strain Ktm-20T belonged to the genus Rhodococcus and is closely related to Rhodococcus triatomae DSM 44892T, Rhodococcus pedocola UC12T, Rhodococcus wratislaviensis NBRC 100605T, Rhodococcus agglutinans CFH S0262T and Rhodococcus canchipurensis MBRL 353T (98.8, 98.7, 98.5, 98.4 and 98.3 % gene sequence similarity, respectively).
29620494	11	16	theme	phylogenetic	1649:1660	arg1	analyses					1662:1669	The morphological, physiological, chemotaxonomic and phylogenetic analyses	1596:1669	The morphological, physiological, chemotaxonomic and phylogenetic analyses	1596:1669	The morphological, physiological, chemotaxonomic and phylogenetic analyses clearly distinguished this strain from its closest phylogenetic neighbours.
29620494	12	17	theme	name	1835:1838	arg1	sp					1857:1858	the name Rhodococcus olei sp	1831:1858	the name Rhodococcus olei sp	1831:1858	Thus, strain Ktm-20T represents a novel species of the genus Rhodococcus, for which the name Rhodococcus olei sp.
29620494	2	18	theme	shaped	170:175	arg1	actinobacterium					177:191	A Gram-stain-positive, non-motile, creamy-white and rod-coccus shaped actinobacterium	107:191	A Gram-stain-positive, non-motile, creamy-white and rod-coccus shaped actinobacterium	107:191	A Gram-stain-positive, non-motile, creamy-white and rod-coccus shaped actinobacterium, designated strain Ktm-20T, capable of degrading petroleum oil was isolated from oil-contaminated soil.
29620494	5	19	dep	Rhodococcus	655:665	arg1	wratislaviensis					667:681	wratislaviensis	667:681	wratislaviensis	667:681	The 16S rRNA gene sequence analysis showed that strain Ktm-20T belonged to the genus Rhodococcus and is closely related to Rhodococcus triatomae DSM 44892T, Rhodococcus pedocola UC12T, Rhodococcus wratislaviensis NBRC 100605T, Rhodococcus agglutinans CFH S0262T and Rhodococcus canchipurensis MBRL 353T (98.8, 98.7, 98.5, 98.4 and 98.3 % gene sequence similarity, respectively).
29620494	5	20	theme	CFH	721:723	arg1	S0262T					725:730	Rhodococcus agglutinans CFH S0262T	697:730	Rhodococcus agglutinans CFH S0262T	697:730	The 16S rRNA gene sequence analysis showed that strain Ktm-20T belonged to the genus Rhodococcus and is closely related to Rhodococcus triatomae DSM 44892T, Rhodococcus pedocola UC12T, Rhodococcus wratislaviensis NBRC 100605T, Rhodococcus agglutinans CFH S0262T and Rhodococcus canchipurensis MBRL 353T (98.8, 98.7, 98.5, 98.4 and 98.3 % gene sequence similarity, respectively).
29620494	12	21	theme	olei	1852:1855	arg1	sp					1857:1858	the name Rhodococcus olei sp	1831:1858	the name Rhodococcus olei sp	1831:1858	Thus, strain Ktm-20T represents a novel species of the genus Rhodococcus, for which the name Rhodococcus olei sp.
29620494	3	22	dep	%	372:372	arg1	w/v					375:377	w/v	375:377	w/v	375:377	Strain Ktm-20T was able to grow at 15-37 °C, at pH 5.5-10.0 and at 0.0-2.0 % (w/v) NaCl concentration.
29620494	1	23	theme	petroleum	55:63	arg1	oil					65:67	petroleum oil	55:67	petroleum oil	55:67	nov., with the ability to degrade petroleum oil, isolated from oil-contaminated soil.
29620494	7	24	theme	diagnostic	1263:1272	arg1	galactose					1205:1213	galactose	1205:1213	galactose	1205:1213	The cell-wall peptidoglycan contained meso-diaminopimelic acid; and galactose, glucose, arabinose and ribose were detected as diagnostic sugars from whole-cell hydrolysates.
29620494	7	24	theme	diagnostic	1263:1272	arg1	sugars					1274:1279	diagnostic sugars	1263:1279	diagnostic sugars from whole-cell hydrolysates	1263:1308	The cell-wall peptidoglycan contained meso-diaminopimelic acid; and galactose, glucose, arabinose and ribose were detected as diagnostic sugars from whole-cell hydrolysates.
29620494	7	24	theme	diagnostic	1263:1272	arg1	ribose					1239:1244	ribose	1239:1244	ribose	1239:1244	The cell-wall peptidoglycan contained meso-diaminopimelic acid; and galactose, glucose, arabinose and ribose were detected as diagnostic sugars from whole-cell hydrolysates.
29620494	7	24	theme	diagnostic	1263:1272	arg1	arabinose					1225:1233	arabinose	1225:1233	arabinose	1225:1233	The cell-wall peptidoglycan contained meso-diaminopimelic acid; and galactose, glucose, arabinose and ribose were detected as diagnostic sugars from whole-cell hydrolysates.
29620494	7	24	theme	diagnostic	1263:1272	arg1	glucose					1216:1222	glucose	1216:1222	glucose	1216:1222	The cell-wall peptidoglycan contained meso-diaminopimelic acid; and galactose, glucose, arabinose and ribose were detected as diagnostic sugars from whole-cell hydrolysates.
29620494	5	25	dep	Rhodococcus	627:637	arg1	pedocola					639:646	pedocola	639:646	pedocola	639:646	The 16S rRNA gene sequence analysis showed that strain Ktm-20T belonged to the genus Rhodococcus and is closely related to Rhodococcus triatomae DSM 44892T, Rhodococcus pedocola UC12T, Rhodococcus wratislaviensis NBRC 100605T, Rhodococcus agglutinans CFH S0262T and Rhodococcus canchipurensis MBRL 353T (98.8, 98.7, 98.5, 98.4 and 98.3 % gene sequence similarity, respectively).
29620494	4	26	theme	polyphasic	449:458	arg1	approach					460:467	a polyphasic approach	447:467	a polyphasic approach	447:467	This strain was taxonomically characterized by a polyphasic approach.
29620494	10	27	theme	relatedness	1387:1397	arg1	values					1399:1404	The DNA-DNA relatedness values	1375:1404	The DNA-DNA relatedness values between strain Ktm-20T and closely related species of the genus Rhodococcus	1375:1480	The DNA-DNA relatedness values between strain Ktm-20T and closely related species of the genus Rhodococcus were between 38.3-25.3 %, which falls below the threshold value of 70 % for the strain to be considered as novel.
29620494	5	28	theme	Rhodococcus	736:746	arg1	353T					768:771	Rhodococcus canchipurensis MBRL 353T	736:771	Rhodococcus canchipurensis MBRL 353T (98.8, 98.7, 98.5, 98.4 and 98.3 % gene sequence similarity, respectively)	736:846	The 16S rRNA gene sequence analysis showed that strain Ktm-20T belonged to the genus Rhodococcus and is closely related to Rhodococcus triatomae DSM 44892T, Rhodococcus pedocola UC12T, Rhodococcus wratislaviensis NBRC 100605T, Rhodococcus agglutinans CFH S0262T and Rhodococcus canchipurensis MBRL 353T (98.8, 98.7, 98.5, 98.4 and 98.3 % gene sequence similarity, respectively).
29620494	8	29	theme	Mycolic	1311:1317	arg1	acids					1319:1323	Mycolic acids	1311:1323	Mycolic acids	1311:1323	Mycolic acids were detected.
29620494	3	30	theme	NaCl	380:383	arg1	concentration					385:397	0.0-2.0 % (w/v) NaCl concentration	364:397	0.0-2.0 % (w/v) NaCl concentration	364:397	Strain Ktm-20T was able to grow at 15-37 °C, at pH 5.5-10.0 and at 0.0-2.0 % (w/v) NaCl concentration.
29620494	0	31	theme	Rhodococcus	0:10	arg1	sp					17:18	Rhodococcus olei sp	0:18	Rhodococcus olei sp.	0:19	Rhodococcus olei sp.
29620494	5	32	theme	genus	549:553	arg1	Rhodococcus					555:565	the genus Rhodococcus	545:565	the genus Rhodococcus	545:565	The 16S rRNA gene sequence analysis showed that strain Ktm-20T belonged to the genus Rhodococcus and is closely related to Rhodococcus triatomae DSM 44892T, Rhodococcus pedocola UC12T, Rhodococcus wratislaviensis NBRC 100605T, Rhodococcus agglutinans CFH S0262T and Rhodococcus canchipurensis MBRL 353T (98.8, 98.7, 98.5, 98.4 and 98.3 % gene sequence similarity, respectively).
29620494	6	33	theme	predominant	1035:1045	arg1	C16 					1064:1067	C16 	1064:1067	C16 	1064:1067	The only respiratory quinone was MK-8(H2); the major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside; and the predominant fatty acids were C16 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and C18 : 1ω9c.
29620494	6	33	theme	predominant	1035:1045	arg1	acids					1053:1057	the predominant fatty acids	1031:1057	the predominant fatty acids	1031:1057	The only respiratory quinone was MK-8(H2); the major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside; and the predominant fatty acids were C16 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and C18 : 1ω9c.
29620494	2	34	theme	rod-coccus	159:168	arg1	actinobacterium					177:191	A Gram-stain-positive, non-motile, creamy-white and rod-coccus shaped actinobacterium	107:191	A Gram-stain-positive, non-motile, creamy-white and rod-coccus shaped actinobacterium	107:191	A Gram-stain-positive, non-motile, creamy-white and rod-coccus shaped actinobacterium, designated strain Ktm-20T, capable of degrading petroleum oil was isolated from oil-contaminated soil.
29620494	12	35	theme	genus	1802:1806	arg1	Rhodococcus					1808:1818	the genus Rhodococcus	1798:1818	the genus Rhodococcus	1798:1818	Thus, strain Ktm-20T represents a novel species of the genus Rhodococcus, for which the name Rhodococcus olei sp.
29620494	14	36	theme	type	1883:1886	arg1	Ktm-20T					1898:1904	Ktm-20T	1898:1904	Ktm-20T (=KEMB 9005-695T=KACC 19390T=JCM 32206T)	1898:1945	The type strain is Ktm-20T (=KEMB 9005-695T=KACC 19390T=JCM 32206T).
29620494	14	36	theme	type	1883:1886	arg1	strain					1888:1893	The type strain	1879:1893	The type strain	1879:1893	The type strain is Ktm-20T (=KEMB 9005-695T=KACC 19390T=JCM 32206T).
29620494	3	37	theme	%	372:372	arg1	concentration					385:397	0.0-2.0 % (w/v) NaCl concentration	364:397	0.0-2.0 % (w/v) NaCl concentration	364:397	Strain Ktm-20T was able to grow at 15-37 °C, at pH 5.5-10.0 and at 0.0-2.0 % (w/v) NaCl concentration.
29620494	2	38	theme	creamy-white	142:153	arg1	actinobacterium					177:191	A Gram-stain-positive, non-motile, creamy-white and rod-coccus shaped actinobacterium	107:191	A Gram-stain-positive, non-motile, creamy-white and rod-coccus shaped actinobacterium	107:191	A Gram-stain-positive, non-motile, creamy-white and rod-coccus shaped actinobacterium, designated strain Ktm-20T, capable of degrading petroleum oil was isolated from oil-contaminated soil.
29620494	6	39	dep	feature	1080:1086	arg1	C16 					1091:1094	C16 	1091:1094	C16 	1091:1094	The only respiratory quinone was MK-8(H2); the major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside; and the predominant fatty acids were C16 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and C18 : 1ω9c.
29620494	12	40	theme	novel	1781:1785	arg1	species					1787:1793	a novel species	1779:1793	a novel species	1779:1793	Thus, strain Ktm-20T represents a novel species of the genus Rhodococcus, for which the name Rhodococcus olei sp.
29620494	6	41	theme	summed	1073:1078	arg1	 0					1069:1070	 0	1069:1070	 0	1069:1070	The only respiratory quinone was MK-8(H2); the major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside; and the predominant fatty acids were C16 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and C18 : 1ω9c.
29620494	6	41	theme	summed	1073:1078	arg1	feature					1080:1086	summed feature 3	1073:1088	summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c)	1073:1119	The only respiratory quinone was MK-8(H2); the major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside; and the predominant fatty acids were C16 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and C18 : 1ω9c.
29620494	10	42	theme	Rhodococcus	1470:1480	arg1	species					1449:1455	closely related species	1433:1455	closely related species	1433:1455	The DNA-DNA relatedness values between strain Ktm-20T and closely related species of the genus Rhodococcus were between 38.3-25.3 %, which falls below the threshold value of 70 % for the strain to be considered as novel.
29620494	10	42	theme	Rhodococcus	1470:1480	arg1	Ktm-20T					1421:1427	strain Ktm-20T	1414:1427	strain Ktm-20T	1414:1427	The DNA-DNA relatedness values between strain Ktm-20T and closely related species of the genus Rhodococcus were between 38.3-25.3 %, which falls below the threshold value of 70 % for the strain to be considered as novel.
29620494	9	43	theme	G+C	1348:1350	arg1	content					1352:1358	The DNA G+C content	1340:1358	The DNA G+C content	1340:1358	The DNA G+C content was 70.9 mol%.
29620494	9	43	theme	G+C	1348:1350	arg1	%					1372:1372	70.9 mol%	1364:1372	70.9 mol%	1364:1372	The DNA G+C content was 70.9 mol%.
29620494	7	44	theme	meso-diaminopimelic	1175:1193	arg1	acid					1195:1198	meso-diaminopimelic acid	1175:1198	meso-diaminopimelic acid	1175:1198	The cell-wall peptidoglycan contained meso-diaminopimelic acid; and galactose, glucose, arabinose and ribose were detected as diagnostic sugars from whole-cell hydrolysates.
29620494	5	45	theme	Rhodococcus	627:637	arg1	UC12T					648:652	Rhodococcus pedocola UC12T	627:652	Rhodococcus pedocola UC12T	627:652	The 16S rRNA gene sequence analysis showed that strain Ktm-20T belonged to the genus Rhodococcus and is closely related to Rhodococcus triatomae DSM 44892T, Rhodococcus pedocola UC12T, Rhodococcus wratislaviensis NBRC 100605T, Rhodococcus agglutinans CFH S0262T and Rhodococcus canchipurensis MBRL 353T (98.8, 98.7, 98.5, 98.4 and 98.3 % gene sequence similarity, respectively).
29620494	2	46	theme	non-motile	130:139	arg1	actinobacterium					177:191	A Gram-stain-positive, non-motile, creamy-white and rod-coccus shaped actinobacterium	107:191	A Gram-stain-positive, non-motile, creamy-white and rod-coccus shaped actinobacterium	107:191	A Gram-stain-positive, non-motile, creamy-white and rod-coccus shaped actinobacterium, designated strain Ktm-20T, capable of degrading petroleum oil was isolated from oil-contaminated soil.
29620494	5	47	theme	sequence	813:820	arg1	similarity					822:831	98.8, 98.7, 98.5, 98.4 and 98.3 % gene sequence similarity	774:831	98.8, 98.7, 98.5, 98.4 and 98.3 % gene sequence similarity	774:831	The 16S rRNA gene sequence analysis showed that strain Ktm-20T belonged to the genus Rhodococcus and is closely related to Rhodococcus triatomae DSM 44892T, Rhodococcus pedocola UC12T, Rhodococcus wratislaviensis NBRC 100605T, Rhodococcus agglutinans CFH S0262T and Rhodococcus canchipurensis MBRL 353T (98.8, 98.7, 98.5, 98.4 and 98.3 % gene sequence similarity, respectively).
29620494	6	48	dep	C16 	1091:1094	arg1	 1ω7c					1096:1100	 1ω7c	1096:1100	 1ω7c	1096:1100	The only respiratory quinone was MK-8(H2); the major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside; and the predominant fatty acids were C16 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and C18 : 1ω9c.
29620494	6	48	dep	C16 	1091:1094	arg1	 1ω6c					1114:1118	 1ω6c	1114:1118	 1ω6c	1114:1118	The only respiratory quinone was MK-8(H2); the major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside; and the predominant fatty acids were C16 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and C18 : 1ω9c.
29620494	6	48	dep	C16 	1091:1094	arg1	C16 					1109:1112	C16 	1109:1112	C16 	1109:1112	The only respiratory quinone was MK-8(H2); the major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside; and the predominant fatty acids were C16 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and C18 : 1ω9c.
29620494	10	49	theme	threshold	1530:1538	arg1	value					1540:1544	the threshold value	1526:1544	the threshold value of 70 % for the strain to be considered as novel	1526:1593	The DNA-DNA relatedness values between strain Ktm-20T and closely related species of the genus Rhodococcus were between 38.3-25.3 %, which falls below the threshold value of 70 % for the strain to be considered as novel.
29620494	2	50	theme	Gram-stain-positive	109:127	arg1	actinobacterium					177:191	A Gram-stain-positive, non-motile, creamy-white and rod-coccus shaped actinobacterium	107:191	A Gram-stain-positive, non-motile, creamy-white and rod-coccus shaped actinobacterium	107:191	A Gram-stain-positive, non-motile, creamy-white and rod-coccus shaped actinobacterium, designated strain Ktm-20T, capable of degrading petroleum oil was isolated from oil-contaminated soil.
29620494	5	51	theme	rRNA	478:481	arg1	analysis					497:504	The 16S rRNA gene sequence analysis	470:504	The 16S rRNA gene sequence analysis	470:504	The 16S rRNA gene sequence analysis showed that strain Ktm-20T belonged to the genus Rhodococcus and is closely related to Rhodococcus triatomae DSM 44892T, Rhodococcus pedocola UC12T, Rhodococcus wratislaviensis NBRC 100605T, Rhodococcus agglutinans CFH S0262T and Rhodococcus canchipurensis MBRL 353T (98.8, 98.7, 98.5, 98.4 and 98.3 % gene sequence similarity, respectively).
29620494	2	52	theme	strain	205:210	arg1	Ktm-20T					212:218	strain Ktm-20T	205:218	strain Ktm-20T	205:218	A Gram-stain-positive, non-motile, creamy-white and rod-coccus shaped actinobacterium, designated strain Ktm-20T, capable of degrading petroleum oil was isolated from oil-contaminated soil.
29620494	5	53	theme	NBRC	683:686	arg1	100605T					688:694	Rhodococcus wratislaviensis NBRC 100605T	655:694	Rhodococcus wratislaviensis NBRC 100605T	655:694	The 16S rRNA gene sequence analysis showed that strain Ktm-20T belonged to the genus Rhodococcus and is closely related to Rhodococcus triatomae DSM 44892T, Rhodococcus pedocola UC12T, Rhodococcus wratislaviensis NBRC 100605T, Rhodococcus agglutinans CFH S0262T and Rhodococcus canchipurensis MBRL 353T (98.8, 98.7, 98.5, 98.4 and 98.3 % gene sequence similarity, respectively).
29620494	6	54	theme	major	896:900	arg1	phosphatidylethanolamine					920:943	phosphatidylethanolamine	920:943	phosphatidylethanolamine	920:943	The only respiratory quinone was MK-8(H2); the major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside; and the predominant fatty acids were C16 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and C18 : 1ω9c.
29620494	6	54	theme	major	896:900	arg1	lipids					908:913	the major polar lipids	892:913	the major polar lipids	892:913	The only respiratory quinone was MK-8(H2); the major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside; and the predominant fatty acids were C16 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and C18 : 1ω9c.
29620494	5	55	dep	353T	768:771	arg1	similarity					822:831	98.8, 98.7, 98.5, 98.4 and 98.3 % gene sequence similarity	774:831	98.8, 98.7, 98.5, 98.4 and 98.3 % gene sequence similarity	774:831	The 16S rRNA gene sequence analysis showed that strain Ktm-20T belonged to the genus Rhodococcus and is closely related to Rhodococcus triatomae DSM 44892T, Rhodococcus pedocola UC12T, Rhodococcus wratislaviensis NBRC 100605T, Rhodococcus agglutinans CFH S0262T and Rhodococcus canchipurensis MBRL 353T (98.8, 98.7, 98.5, 98.4 and 98.3 % gene sequence similarity, respectively).
29620494	10	56	theme	%	1552:1552	arg1	value					1540:1544	the threshold value	1526:1544	the threshold value of 70 % for the strain to be considered as novel	1526:1593	The DNA-DNA relatedness values between strain Ktm-20T and closely related species of the genus Rhodococcus were between 38.3-25.3 %, which falls below the threshold value of 70 % for the strain to be considered as novel.
29620494	11	57	theme	phylogenetic	1722:1733	arg1	neighbours					1735:1744	its closest phylogenetic neighbours	1710:1744	its closest phylogenetic neighbours	1710:1744	The morphological, physiological, chemotaxonomic and phylogenetic analyses clearly distinguished this strain from its closest phylogenetic neighbours.
29620494	5	58	theme	%	806:806	arg1	similarity					822:831	98.8, 98.7, 98.5, 98.4 and 98.3 % gene sequence similarity	774:831	98.8, 98.7, 98.5, 98.4 and 98.3 % gene sequence similarity	774:831	The 16S rRNA gene sequence analysis showed that strain Ktm-20T belonged to the genus Rhodococcus and is closely related to Rhodococcus triatomae DSM 44892T, Rhodococcus pedocola UC12T, Rhodococcus wratislaviensis NBRC 100605T, Rhodococcus agglutinans CFH S0262T and Rhodococcus canchipurensis MBRL 353T (98.8, 98.7, 98.5, 98.4 and 98.3 % gene sequence similarity, respectively).
29620494	5	59	theme	strain	518:523	arg1	Ktm-20T					525:531	strain Ktm-20T	518:531	strain Ktm-20T	518:531	The 16S rRNA gene sequence analysis showed that strain Ktm-20T belonged to the genus Rhodococcus and is closely related to Rhodococcus triatomae DSM 44892T, Rhodococcus pedocola UC12T, Rhodococcus wratislaviensis NBRC 100605T, Rhodococcus agglutinans CFH S0262T and Rhodococcus canchipurensis MBRL 353T (98.8, 98.7, 98.5, 98.4 and 98.3 % gene sequence similarity, respectively).
29620494	1	60	theme	oil-contaminated	84:99	arg1	soil					101:104	oil-contaminated soil	84:104	oil-contaminated soil	84:104	nov., with the ability to degrade petroleum oil, isolated from oil-contaminated soil.
29620494	2	61	attach	isolated	260:267	arg1	soil					291:294	oil-contaminated soil	274:294	oil-contaminated soil	274:294	A Gram-stain-positive, non-motile, creamy-white and rod-coccus shaped actinobacterium, designated strain Ktm-20T, capable of degrading petroleum oil was isolated from oil-contaminated soil.
29620494	2	61	attach	isolated	260:267	arg2	actinobacterium					177:191	A Gram-stain-positive, non-motile, creamy-white and rod-coccus shaped actinobacterium	107:191	A Gram-stain-positive, non-motile, creamy-white and rod-coccus shaped actinobacterium	107:191	A Gram-stain-positive, non-motile, creamy-white and rod-coccus shaped actinobacterium, designated strain Ktm-20T, capable of degrading petroleum oil was isolated from oil-contaminated soil.
29620494	5	62	theme	Rhodococcus	655:665	arg1	100605T					688:694	Rhodococcus wratislaviensis NBRC 100605T	655:694	Rhodococcus wratislaviensis NBRC 100605T	655:694	The 16S rRNA gene sequence analysis showed that strain Ktm-20T belonged to the genus Rhodococcus and is closely related to Rhodococcus triatomae DSM 44892T, Rhodococcus pedocola UC12T, Rhodococcus wratislaviensis NBRC 100605T, Rhodococcus agglutinans CFH S0262T and Rhodococcus canchipurensis MBRL 353T (98.8, 98.7, 98.5, 98.4 and 98.3 % gene sequence similarity, respectively).
29620494	6	63	theme	phosphatidylinositol	995:1014	arg1	mannoside					1016:1024	phosphatidylinositol mannoside	995:1024	phosphatidylinositol mannoside	995:1024	The only respiratory quinone was MK-8(H2); the major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside; and the predominant fatty acids were C16 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and C18 : 1ω9c.
29620494	3	64	theme	Strain	297:302	arg1	Ktm-20T					304:310	Strain Ktm-20T	297:310	Strain Ktm-20T	297:310	Strain Ktm-20T was able to grow at 15-37 °C, at pH 5.5-10.0 and at 0.0-2.0 % (w/v) NaCl concentration.
29620494	7	65	theme	cell-wall	1141:1149	arg1	peptidoglycan					1151:1163	The cell-wall peptidoglycan	1137:1163	The cell-wall peptidoglycan	1137:1163	The cell-wall peptidoglycan contained meso-diaminopimelic acid; and galactose, glucose, arabinose and ribose were detected as diagnostic sugars from whole-cell hydrolysates.
29620494	2	66	theme	petroleum	242:250	arg1	oil					252:254	petroleum oil	242:254	petroleum oil	242:254	A Gram-stain-positive, non-motile, creamy-white and rod-coccus shaped actinobacterium, designated strain Ktm-20T, capable of degrading petroleum oil was isolated from oil-contaminated soil.
29620494	5	67	theme	sequence	488:495	arg1	analysis					497:504	The 16S rRNA gene sequence analysis	470:504	The 16S rRNA gene sequence analysis	470:504	The 16S rRNA gene sequence analysis showed that strain Ktm-20T belonged to the genus Rhodococcus and is closely related to Rhodococcus triatomae DSM 44892T, Rhodococcus pedocola UC12T, Rhodococcus wratislaviensis NBRC 100605T, Rhodococcus agglutinans CFH S0262T and Rhodococcus canchipurensis MBRL 353T (98.8, 98.7, 98.5, 98.4 and 98.3 % gene sequence similarity, respectively).
29620494	5	68	dep	Rhodococcus	697:707	arg1	agglutinans					709:719	agglutinans	709:719	agglutinans	709:719	The 16S rRNA gene sequence analysis showed that strain Ktm-20T belonged to the genus Rhodococcus and is closely related to Rhodococcus triatomae DSM 44892T, Rhodococcus pedocola UC12T, Rhodococcus wratislaviensis NBRC 100605T, Rhodococcus agglutinans CFH S0262T and Rhodococcus canchipurensis MBRL 353T (98.8, 98.7, 98.5, 98.4 and 98.3 % gene sequence similarity, respectively).
29620494	5	69	theme	MBRL	763:766	arg1	353T					768:771	Rhodococcus canchipurensis MBRL 353T	736:771	Rhodococcus canchipurensis MBRL 353T (98.8, 98.7, 98.5, 98.4 and 98.3 % gene sequence similarity, respectively)	736:846	The 16S rRNA gene sequence analysis showed that strain Ktm-20T belonged to the genus Rhodococcus and is closely related to Rhodococcus triatomae DSM 44892T, Rhodococcus pedocola UC12T, Rhodococcus wratislaviensis NBRC 100605T, Rhodococcus agglutinans CFH S0262T and Rhodococcus canchipurensis MBRL 353T (98.8, 98.7, 98.5, 98.4 and 98.3 % gene sequence similarity, respectively).
29620494	7	70	contain	contained	1165:1173	arg2	acid					1195:1198	meso-diaminopimelic acid	1175:1198	meso-diaminopimelic acid	1175:1198	The cell-wall peptidoglycan contained meso-diaminopimelic acid; and galactose, glucose, arabinose and ribose were detected as diagnostic sugars from whole-cell hydrolysates.
29620494	7	70	contain	contained	1165:1173	arg1	peptidoglycan					1151:1163	The cell-wall peptidoglycan	1137:1163	The cell-wall peptidoglycan	1137:1163	The cell-wall peptidoglycan contained meso-diaminopimelic acid; and galactose, glucose, arabinose and ribose were detected as diagnostic sugars from whole-cell hydrolysates.
29620494	12	71	theme	Rhodococcus	1840:1850	arg1	sp					1857:1858	the name Rhodococcus olei sp	1831:1858	the name Rhodococcus olei sp	1831:1858	Thus, strain Ktm-20T represents a novel species of the genus Rhodococcus, for which the name Rhodococcus olei sp.
29620494	14	72	theme	19390T=JCM	1928:1937	arg1	32206T					1939:1944	=KEMB 9005-695T=KACC 19390T=JCM 32206T	1907:1944	=KEMB 9005-695T=KACC 19390T=JCM 32206T	1907:1944	The type strain is Ktm-20T (=KEMB 9005-695T=KACC 19390T=JCM 32206T).
29620494	14	72	theme	19390T=JCM	1928:1937	arg1	Ktm-20T					1898:1904	Ktm-20T	1898:1904	Ktm-20T (=KEMB 9005-695T=KACC 19390T=JCM 32206T)	1898:1945	The type strain is Ktm-20T (=KEMB 9005-695T=KACC 19390T=JCM 32206T).
29620494	0	73	theme	olei	12:15	arg1	sp					17:18	Rhodococcus olei sp	0:18	Rhodococcus olei sp.	0:19	Rhodococcus olei sp.
29620494	14	74	theme	=KEMB	1907:1911	arg1	32206T					1939:1944	=KEMB 9005-695T=KACC 19390T=JCM 32206T	1907:1944	=KEMB 9005-695T=KACC 19390T=JCM 32206T	1907:1944	The type strain is Ktm-20T (=KEMB 9005-695T=KACC 19390T=JCM 32206T).
29620494	14	74	theme	=KEMB	1907:1911	arg1	Ktm-20T					1898:1904	Ktm-20T	1898:1904	Ktm-20T (=KEMB 9005-695T=KACC 19390T=JCM 32206T)	1898:1945	The type strain is Ktm-20T (=KEMB 9005-695T=KACC 19390T=JCM 32206T).
29620494	7	75	from	hydrolysates	1297:1308	arg1	galactose					1205:1213	galactose	1205:1213	galactose	1205:1213	The cell-wall peptidoglycan contained meso-diaminopimelic acid; and galactose, glucose, arabinose and ribose were detected as diagnostic sugars from whole-cell hydrolysates.
29620494	7	75	from	hydrolysates	1297:1308	arg1	sugars					1274:1279	diagnostic sugars	1263:1279	diagnostic sugars from whole-cell hydrolysates	1263:1308	The cell-wall peptidoglycan contained meso-diaminopimelic acid; and galactose, glucose, arabinose and ribose were detected as diagnostic sugars from whole-cell hydrolysates.
29620494	7	75	from	hydrolysates	1297:1308	arg1	ribose					1239:1244	ribose	1239:1244	ribose	1239:1244	The cell-wall peptidoglycan contained meso-diaminopimelic acid; and galactose, glucose, arabinose and ribose were detected as diagnostic sugars from whole-cell hydrolysates.
29620494	7	75	from	hydrolysates	1297:1308	arg1	arabinose					1225:1233	arabinose	1225:1233	arabinose	1225:1233	The cell-wall peptidoglycan contained meso-diaminopimelic acid; and galactose, glucose, arabinose and ribose were detected as diagnostic sugars from whole-cell hydrolysates.
29620494	7	75	from	hydrolysates	1297:1308	arg1	glucose					1216:1222	glucose	1216:1222	glucose	1216:1222	The cell-wall peptidoglycan contained meso-diaminopimelic acid; and galactose, glucose, arabinose and ribose were detected as diagnostic sugars from whole-cell hydrolysates.
29620494	6	76	theme	fatty	1047:1051	arg1	C16 					1064:1067	C16 	1064:1067	C16 	1064:1067	The only respiratory quinone was MK-8(H2); the major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside; and the predominant fatty acids were C16 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and C18 : 1ω9c.
29620494	6	76	theme	fatty	1047:1051	arg1	acids					1053:1057	the predominant fatty acids	1031:1057	the predominant fatty acids	1031:1057	The only respiratory quinone was MK-8(H2); the major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside; and the predominant fatty acids were C16 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and C18 : 1ω9c.
29620494	3	77	theme	0.0-2.0 	364:371	arg1	%					372:372	%	372:372	%	372:372	Strain Ktm-20T was able to grow at 15-37 °C, at pH 5.5-10.0 and at 0.0-2.0 % (w/v) NaCl concentration.
29620494	10	78	theme	DNA-DNA	1379:1385	arg1	values					1399:1404	The DNA-DNA relatedness values	1375:1404	The DNA-DNA relatedness values between strain Ktm-20T and closely related species of the genus Rhodococcus	1375:1480	The DNA-DNA relatedness values between strain Ktm-20T and closely related species of the genus Rhodococcus were between 38.3-25.3 %, which falls below the threshold value of 70 % for the strain to be considered as novel.
29620494	11	79	theme	morphological	1600:1612	arg1	analyses					1662:1669	The morphological, physiological, chemotaxonomic and phylogenetic analyses	1596:1669	The morphological, physiological, chemotaxonomic and phylogenetic analyses	1596:1669	The morphological, physiological, chemotaxonomic and phylogenetic analyses clearly distinguished this strain from its closest phylogenetic neighbours.
29620494	12	80	theme	Rhodococcus	1808:1818	arg1	species					1787:1793	a novel species	1779:1793	a novel species	1779:1793	Thus, strain Ktm-20T represents a novel species of the genus Rhodococcus, for which the name Rhodococcus olei sp.
29620494	10	81	theme	strain	1414:1419	arg1	Ktm-20T					1421:1427	strain Ktm-20T	1414:1427	strain Ktm-20T	1414:1427	The DNA-DNA relatedness values between strain Ktm-20T and closely related species of the genus Rhodococcus were between 38.3-25.3 %, which falls below the threshold value of 70 % for the strain to be considered as novel.
29620494	6	82	dep	C16 	1064:1067	arg1	 0					1069:1070	 0	1069:1070	 0	1069:1070	The only respiratory quinone was MK-8(H2); the major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside; and the predominant fatty acids were C16 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and C18 : 1ω9c.
29620494	6	82	dep	C16 	1064:1067	arg1	feature					1080:1086	summed feature 3	1073:1088	summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c)	1073:1119	The only respiratory quinone was MK-8(H2); the major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside; and the predominant fatty acids were C16 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and C18 : 1ω9c.
29620494	5	83	dep	Rhodococcus	593:603	arg1	triatomae					605:613	triatomae	605:613	triatomae	605:613	The 16S rRNA gene sequence analysis showed that strain Ktm-20T belonged to the genus Rhodococcus and is closely related to Rhodococcus triatomae DSM 44892T, Rhodococcus pedocola UC12T, Rhodococcus wratislaviensis NBRC 100605T, Rhodococcus agglutinans CFH S0262T and Rhodococcus canchipurensis MBRL 353T (98.8, 98.7, 98.5, 98.4 and 98.3 % gene sequence similarity, respectively).
29620494	14	84	theme	9005-695T=KACC	1913:1926	arg1	32206T					1939:1944	=KEMB 9005-695T=KACC 19390T=JCM 32206T	1907:1944	=KEMB 9005-695T=KACC 19390T=JCM 32206T	1907:1944	The type strain is Ktm-20T (=KEMB 9005-695T=KACC 19390T=JCM 32206T).
29620494	14	84	theme	9005-695T=KACC	1913:1926	arg1	Ktm-20T					1898:1904	Ktm-20T	1898:1904	Ktm-20T (=KEMB 9005-695T=KACC 19390T=JCM 32206T)	1898:1945	The type strain is Ktm-20T (=KEMB 9005-695T=KACC 19390T=JCM 32206T).
29620494	11	85	theme	chemotaxonomic	1630:1643	arg1	analyses					1662:1669	The morphological, physiological, chemotaxonomic and phylogenetic analyses	1596:1669	The morphological, physiological, chemotaxonomic and phylogenetic analyses	1596:1669	The morphological, physiological, chemotaxonomic and phylogenetic analyses clearly distinguished this strain from its closest phylogenetic neighbours.
29620494	12	86	theme	strain	1753:1758	arg1	Ktm-20T					1760:1766	strain Ktm-20T	1753:1766	strain Ktm-20T	1753:1766	Thus, strain Ktm-20T represents a novel species of the genus Rhodococcus, for which the name Rhodococcus olei sp.
29620494	10	87	theme	related	1441:1447	arg1	species					1449:1455	closely related species	1433:1455	closely related species	1433:1455	The DNA-DNA relatedness values between strain Ktm-20T and closely related species of the genus Rhodococcus were between 38.3-25.3 %, which falls below the threshold value of 70 % for the strain to be considered as novel.
29620494	11	88	theme	physiological	1615:1627	arg1	analyses					1662:1669	The morphological, physiological, chemotaxonomic and phylogenetic analyses	1596:1669	The morphological, physiological, chemotaxonomic and phylogenetic analyses	1596:1669	The morphological, physiological, chemotaxonomic and phylogenetic analyses clearly distinguished this strain from its closest phylogenetic neighbours.
29620494	9	89	theme	DNA	1344:1346	arg1	content					1352:1358	The DNA G+C content	1340:1358	The DNA G+C content	1340:1358	The DNA G+C content was 70.9 mol%.
29620494	9	89	theme	DNA	1344:1346	arg1	%					1372:1372	70.9 mol%	1364:1372	70.9 mol%	1364:1372	The DNA G+C content was 70.9 mol%.
29617463	11	0	from	inoculation	1847:1857	arg1	mice					1871:1874	ASF T5KO mice	1862:1874	ASF T5KO mice	1862:1874	AIEC LF82 inoculation in ASF T5KO mice resulted in microbiota components having elevated levels of bioactive lipopolysaccharide and flagellin, a modest level of low-grade inflammation and increased adiposity.
29617463	3	1	theme	gut	710:712	arg1	inflammation					714:725	gut inflammation	710:725	gut inflammation	710:725	That the complete absence of a microbiota (i.e. germfree conditions) eliminates all evidence of inflammation in TLR5-deficient mice demonstrates that this model of gut inflammation is microbiota-dependent.
29617463	8	2	theme	marker	1410:1415	arg1	loads					1338:1342	bacterial loads	1328:1342	bacterial loads	1328:1342	Feces were assayed for bacterial loads, fecal lipopolysaccharide and flagellin loads, fecal inflammatory marker lipocalin-2 and microbiota composition.
29617463	8	2	theme	marker	1410:1415	arg1	lipocalin-2					1417:1427	fecal inflammatory marker lipocalin-2	1391:1427	fecal inflammatory marker lipocalin-2	1391:1427	Feces were assayed for bacterial loads, fecal lipopolysaccharide and flagellin loads, fecal inflammatory marker lipocalin-2 and microbiota composition.
29617463	10	3	theme	T5KO	1795:1798	arg1	mice					1800:1803	T5KO mice	1795:1803	T5KO mice	1795:1803	Concomitantly, the ASF microbial community was similar between WT and T5KO mice, while inoculation with AIEC strain LF82 resulted in alteration of the ASF community in T5KO mice compared to WT control animals.
29617463	9	4	theme	maintained	1487:1496	arg1	mice					1501:1504	similarly maintained WT mice	1477:1504	similarly maintained WT mice	1477:1504	RESULTS Relative to similarly maintained WT mice, mice lacking TLR5 (T5KO) did not display low-grade intestinal inflammation nor metabolic syndrome under ASF conditions.
29617463	12	5	theme	limited-complexity	2063:2080	arg1	microbiota					2098:2107	a limited-complexity pathobiont-free microbiota	2061:2107	a limited-complexity pathobiont-free microbiota	2061:2107	CONCLUSIONS In a limited-complexity pathobiont-free microbiota, loss of the flagellin receptor TLR5 does not impact microbiota composition nor its ability to promote inflammation.
29617463	10	6	theme	T5KO	1697:1700	arg1	mice					1702:1705	WT and T5KO mice	1690:1705	WT and T5KO mice	1690:1705	Concomitantly, the ASF microbial community was similar between WT and T5KO mice, while inoculation with AIEC strain LF82 resulted in alteration of the ASF community in T5KO mice compared to WT control animals.
29617463	3	7	theme	complete	555:562	arg1	absence					564:570	the complete absence	551:570	the complete absence of a microbiota (i.e. germfree conditions)	551:613	That the complete absence of a microbiota (i.e. germfree conditions) eliminates all evidence of inflammation in TLR5-deficient mice demonstrates that this model of gut inflammation is microbiota-dependent.
29617463	1	8	theme	beneficial	183:192	arg1	microbiota					194:203	a stable and beneficial microbiota	170:203	a stable and beneficial microbiota	170:203	BACKGROUND Inability to maintain a stable and beneficial microbiota is associated with chronic gut inflammation, which classically manifests as colitis but may more commonly exist as low-grade inflammation that promotes metabolic syndrome.
29617463	2	9	from	Alterations	377:387	arg1	microbiota					392:401	microbiota	392:401	microbiota	392:401	Alterations in microbiota, and associated inflammation, can originate from dysfunction in host proteins that manage the microbiota, such as the flagellin receptor TLR5.
29617463	6	10	theme	Schaedler	1202:1210	arg1	"					1217:1217	"Altered Schaedler Flora"	1193:1217	"Altered Schaedler Flora" (ASF)	1193:1223	For this purpose, WT and TLR5-deficient mice were generated and maintained with the 8-member consortium of bacteria referred to as "Altered Schaedler Flora" (ASF).
29617463	6	10	theme	Schaedler	1202:1210	arg1	ASF					1220:1222	ASF	1220:1222	ASF	1220:1222	For this purpose, WT and TLR5-deficient mice were generated and maintained with the 8-member consortium of bacteria referred to as "Altered Schaedler Flora" (ASF).
29617463	10	11	theme	microbial	1650:1658	arg1	similar					1674:1680	similar	1674:1680	similar	1674:1680	Concomitantly, the ASF microbial community was similar between WT and T5KO mice, while inoculation with AIEC strain LF82 resulted in alteration of the ASF community in T5KO mice compared to WT control animals.
29617463	10	11	theme	microbial	1650:1658	arg1	community					1660:1668	the ASF microbial community	1642:1668	the ASF microbial community	1642:1668	Concomitantly, the ASF microbial community was similar between WT and T5KO mice, while inoculation with AIEC strain LF82 resulted in alteration of the ASF community in T5KO mice compared to WT control animals.
29617463	11	12	theme	AIEC	1837:1840	arg1	inoculation					1847:1857	AIEC LF82 inoculation	1837:1857	AIEC LF82 inoculation in ASF T5KO mice	1837:1874	AIEC LF82 inoculation in ASF T5KO mice resulted in microbiota components having elevated levels of bioactive lipopolysaccharide and flagellin, a modest level of low-grade inflammation and increased adiposity.
29617463	10	13	theme	WT	1817:1818	arg1	animals					1828:1834	WT control animals	1817:1834	WT control animals	1817:1834	Concomitantly, the ASF microbial community was similar between WT and T5KO mice, while inoculation with AIEC strain LF82 resulted in alteration of the ASF community in T5KO mice compared to WT control animals.
29617463	8	14	theme	inflammatory	1397:1408	arg1	loads					1338:1342	bacterial loads	1328:1342	bacterial loads	1328:1342	Feces were assayed for bacterial loads, fecal lipopolysaccharide and flagellin loads, fecal inflammatory marker lipocalin-2 and microbiota composition.
29617463	8	14	theme	inflammatory	1397:1408	arg1	lipocalin-2					1417:1427	fecal inflammatory marker lipocalin-2	1391:1427	fecal inflammatory marker lipocalin-2	1391:1427	Feces were assayed for bacterial loads, fecal lipopolysaccharide and flagellin loads, fecal inflammatory marker lipocalin-2 and microbiota composition.
29617463	3	15	theme	microbiota	577:586	arg1	absence					564:570	the complete absence	551:570	the complete absence of a microbiota (i.e. germfree conditions)	551:613	That the complete absence of a microbiota (i.e. germfree conditions) eliminates all evidence of inflammation in TLR5-deficient mice demonstrates that this model of gut inflammation is microbiota-dependent.
29617463	11	16	theme	ASF	1862:1864	arg1	mice					1871:1874	ASF T5KO mice	1862:1874	ASF T5KO mice	1862:1874	AIEC LF82 inoculation in ASF T5KO mice resulted in microbiota components having elevated levels of bioactive lipopolysaccharide and flagellin, a modest level of low-grade inflammation and increased adiposity.
29617463	11	17	theme	inflammation	2008:2019	arg1	level					1989:1993	a modest level	1980:1993	a modest level of low-grade inflammation	1980:2019	AIEC LF82 inoculation in ASF T5KO mice resulted in microbiota components having elevated levels of bioactive lipopolysaccharide and flagellin, a modest level of low-grade inflammation and increased adiposity.
29617463	11	17	theme	inflammation	2008:2019	arg1	adiposity					2035:2043	increased adiposity	2025:2043	increased adiposity	2025:2043	AIEC LF82 inoculation in ASF T5KO mice resulted in microbiota components having elevated levels of bioactive lipopolysaccharide and flagellin, a modest level of low-grade inflammation and increased adiposity.
29617463	11	17	theme	inflammation	2008:2019	arg1	levels					1926:1931	elevated levels	1917:1931	elevated levels of bioactive lipopolysaccharide and flagellin	1917:1977	AIEC LF82 inoculation in ASF T5KO mice resulted in microbiota components having elevated levels of bioactive lipopolysaccharide and flagellin, a modest level of low-grade inflammation and increased adiposity.
29617463	12	18	from	impact	2155:2160	arg1	CONCLUSIONS					2046:2056	CONCLUSIONS	2046:2056	CONCLUSIONS	2046:2056	CONCLUSIONS In a limited-complexity pathobiont-free microbiota, loss of the flagellin receptor TLR5 does not impact microbiota composition nor its ability to promote inflammation.
29617463	1	19	theme	gut	232:234	arg1	inflammation					236:247	chronic gut inflammation	224:247	chronic gut inflammation	224:247	BACKGROUND Inability to maintain a stable and beneficial microbiota is associated with chronic gut inflammation, which classically manifests as colitis but may more commonly exist as low-grade inflammation that promotes metabolic syndrome.
29617463	13	20	theme	flagellin	2337:2345	arg1	levels					2304:2309	levels	2304:2309	levels of lipopolysaccharide and flagellin	2304:2345	Addition of AIEC to this ecosystem perturbs microbiota composition, increases levels of lipopolysaccharide and flagellin, but only modestly promotes gut inflammation and adiposity, suggesting that the phenotypes previously associated with loss of this innate immune receptor require disruption of complex microbiota.
29617463	3	21	theme	inflammation	642:653	arg1	evidence					630:637	all evidence	626:637	all evidence of inflammation in TLR5-deficient mice	626:676	That the complete absence of a microbiota (i.e. germfree conditions) eliminates all evidence of inflammation in TLR5-deficient mice demonstrates that this model of gut inflammation is microbiota-dependent.
29617463	5	22	theme	associated	955:964	arg1	inflammation					966:977	microbiota mismanagement and associated inflammation	926:977	microbiota mismanagement and associated inflammation in TLR5-deficient mice	926:1000	Herein, we examined the extent to which microbiota mismanagement and associated inflammation in TLR5-deficient mice would manifest in a limited and pathobiont-free microbiota.
29617463	2	23	theme	flagellin	521:529	arg1	TLR5					540:543	the flagellin receptor TLR5	517:543	the flagellin receptor TLR5	517:543	Alterations in microbiota, and associated inflammation, can originate from dysfunction in host proteins that manage the microbiota, such as the flagellin receptor TLR5.
29617463	8	24	theme	fecal	1345:1349	arg1	lipopolysaccharide					1351:1368	fecal lipopolysaccharide	1345:1368	fecal lipopolysaccharide	1345:1368	Feces were assayed for bacterial loads, fecal lipopolysaccharide and flagellin loads, fecal inflammatory marker lipocalin-2 and microbiota composition.
29617463	11	25	theme	increased	2025:2033	arg1	adiposity					2035:2043	increased adiposity	2025:2043	increased adiposity	2025:2043	AIEC LF82 inoculation in ASF T5KO mice resulted in microbiota components having elevated levels of bioactive lipopolysaccharide and flagellin, a modest level of low-grade inflammation and increased adiposity.
29617463	9	26	theme	intestinal	1558:1567	arg1	inflammation					1569:1580	low-grade intestinal inflammation	1548:1580	low-grade intestinal inflammation	1548:1580	RESULTS Relative to similarly maintained WT mice, mice lacking TLR5 (T5KO) did not display low-grade intestinal inflammation nor metabolic syndrome under ASF conditions.
29617463	12	27	theme	receptor	2132:2139	arg1	TLR5					2141:2144	the flagellin receptor TLR5	2118:2144	the flagellin receptor TLR5	2118:2144	CONCLUSIONS In a limited-complexity pathobiont-free microbiota, loss of the flagellin receptor TLR5 does not impact microbiota composition nor its ability to promote inflammation.
29617463	1	28	theme	stable	172:177	arg1	microbiota					194:203	a stable and beneficial microbiota	170:203	a stable and beneficial microbiota	170:203	BACKGROUND Inability to maintain a stable and beneficial microbiota is associated with chronic gut inflammation, which classically manifests as colitis but may more commonly exist as low-grade inflammation that promotes metabolic syndrome.
29617463	13	29	theme	AIEC	2238:2241	arg1	Addition					2226:2233	Addition	2226:2233	Addition of AIEC to this ecosystem	2226:2259	Addition of AIEC to this ecosystem perturbs microbiota composition, increases levels of lipopolysaccharide and flagellin, but only modestly promotes gut inflammation and adiposity, suggesting that the phenotypes previously associated with loss of this innate immune receptor require disruption of complex microbiota.
29617463	7	30	theme	ASF	1231:1233	arg1	animals					1235:1241	Such ASF animals	1226:1241	Such ASF animals	1226:1241	Such ASF animals were subsequently inoculated with AIEC reference strain LF82.
29617463	11	31	theme	modest	1982:1987	arg1	level					1989:1993	a modest level	1980:1993	a modest level of low-grade inflammation	1980:2019	AIEC LF82 inoculation in ASF T5KO mice resulted in microbiota components having elevated levels of bioactive lipopolysaccharide and flagellin, a modest level of low-grade inflammation and increased adiposity.
29617463	8	32	theme	fecal	1391:1395	arg1	loads					1338:1342	bacterial loads	1328:1342	bacterial loads	1328:1342	Feces were assayed for bacterial loads, fecal lipopolysaccharide and flagellin loads, fecal inflammatory marker lipocalin-2 and microbiota composition.
29617463	8	32	theme	fecal	1391:1395	arg1	lipocalin-2					1417:1427	fecal inflammatory marker lipocalin-2	1391:1427	fecal inflammatory marker lipocalin-2	1391:1427	Feces were assayed for bacterial loads, fecal lipopolysaccharide and flagellin loads, fecal inflammatory marker lipocalin-2 and microbiota composition.
29617463	3	33	from	evidence	630:637	arg1	mice					673:676	TLR5-deficient mice	658:676	TLR5-deficient mice	658:676	That the complete absence of a microbiota (i.e. germfree conditions) eliminates all evidence of inflammation in TLR5-deficient mice demonstrates that this model of gut inflammation is microbiota-dependent.
29617463	11	34	theme	lipopolysaccharide	1946:1963	arg1	level					1989:1993	a modest level	1980:1993	a modest level of low-grade inflammation	1980:2019	AIEC LF82 inoculation in ASF T5KO mice resulted in microbiota components having elevated levels of bioactive lipopolysaccharide and flagellin, a modest level of low-grade inflammation and increased adiposity.
29617463	11	34	theme	lipopolysaccharide	1946:1963	arg1	adiposity					2035:2043	increased adiposity	2025:2043	increased adiposity	2025:2043	AIEC LF82 inoculation in ASF T5KO mice resulted in microbiota components having elevated levels of bioactive lipopolysaccharide and flagellin, a modest level of low-grade inflammation and increased adiposity.
29617463	11	34	theme	lipopolysaccharide	1946:1963	arg1	levels					1926:1931	elevated levels	1917:1931	elevated levels of bioactive lipopolysaccharide and flagellin	1917:1977	AIEC LF82 inoculation in ASF T5KO mice resulted in microbiota components having elevated levels of bioactive lipopolysaccharide and flagellin, a modest level of low-grade inflammation and increased adiposity.
29617463	0	35	theme	innate	111:116	arg1	deficiency					125:134	an innate immune deficiency	108:134	an innate immune deficiency	108:134	Mice harboring pathobiont-free microbiota do not develop intestinal inflammation that normally results from an innate immune deficiency.
29617463	9	36	theme	ASF	1611:1613	arg1	conditions					1615:1624	ASF conditions	1611:1624	ASF conditions	1611:1624	RESULTS Relative to similarly maintained WT mice, mice lacking TLR5 (T5KO) did not display low-grade intestinal inflammation nor metabolic syndrome under ASF conditions.
29617463	4	37	theme	microbiota	777:786	arg1	dependency					788:797	such microbiota dependency	772:797	such microbiota dependency	772:797	We hypothesize that such microbiota dependency reflects an inability to manage pathobionts, such as Adherent-Invasive E. coli (AIEC).
29617463	2	38	theme	associated	408:417	arg1	inflammation					419:430	associated inflammation	408:430	associated inflammation	408:430	Alterations in microbiota, and associated inflammation, can originate from dysfunction in host proteins that manage the microbiota, such as the flagellin receptor TLR5.
29617463	5	39	theme	TLR5-deficient	982:995	arg1	mice					997:1000	TLR5-deficient mice	982:1000	TLR5-deficient mice	982:1000	Herein, we examined the extent to which microbiota mismanagement and associated inflammation in TLR5-deficient mice would manifest in a limited and pathobiont-free microbiota.
29617463	2	40	theme	host	467:470	arg1	proteins					472:479	host proteins	467:479	host proteins	467:479	Alterations in microbiota, and associated inflammation, can originate from dysfunction in host proteins that manage the microbiota, such as the flagellin receptor TLR5.
29617463	2	40	theme	host	467:470	arg1	TLR5					540:543	the flagellin receptor TLR5	517:543	the flagellin receptor TLR5	517:543	Alterations in microbiota, and associated inflammation, can originate from dysfunction in host proteins that manage the microbiota, such as the flagellin receptor TLR5.
29617463	10	41	theme	AIEC	1731:1734	arg1	strain					1736:1741	AIEC strain LF82	1731:1746	AIEC strain LF82	1731:1746	Concomitantly, the ASF microbial community was similar between WT and T5KO mice, while inoculation with AIEC strain LF82 resulted in alteration of the ASF community in T5KO mice compared to WT control animals.
29617463	0	42	theme	pathobiont-free	15:29	arg1	microbiota					31:40	pathobiont-free microbiota	15:40	pathobiont-free microbiota	15:40	Mice harboring pathobiont-free microbiota do not develop intestinal inflammation that normally results from an innate immune deficiency.
29617463	13	43	theme	lipopolysaccharide	2314:2331	arg1	levels					2304:2309	levels	2304:2309	levels of lipopolysaccharide and flagellin	2304:2345	Addition of AIEC to this ecosystem perturbs microbiota composition, increases levels of lipopolysaccharide and flagellin, but only modestly promotes gut inflammation and adiposity, suggesting that the phenotypes previously associated with loss of this innate immune receptor require disruption of complex microbiota.
29617463	13	44	theme	complex	2523:2529	arg1	microbiota					2531:2540	complex microbiota	2523:2540	complex microbiota	2523:2540	Addition of AIEC to this ecosystem perturbs microbiota composition, increases levels of lipopolysaccharide and flagellin, but only modestly promotes gut inflammation and adiposity, suggesting that the phenotypes previously associated with loss of this innate immune receptor require disruption of complex microbiota.
29617463	6	45	theme	TLR5-deficient	1087:1100	arg1	mice					1102:1105	WT and TLR5-deficient mice	1080:1105	WT and TLR5-deficient mice	1080:1105	For this purpose, WT and TLR5-deficient mice were generated and maintained with the 8-member consortium of bacteria referred to as "Altered Schaedler Flora" (ASF).
29617463	11	46	theme	flagellin	1969:1977	arg1	level					1989:1993	a modest level	1980:1993	a modest level of low-grade inflammation	1980:2019	AIEC LF82 inoculation in ASF T5KO mice resulted in microbiota components having elevated levels of bioactive lipopolysaccharide and flagellin, a modest level of low-grade inflammation and increased adiposity.
29617463	11	46	theme	flagellin	1969:1977	arg1	adiposity					2035:2043	increased adiposity	2025:2043	increased adiposity	2025:2043	AIEC LF82 inoculation in ASF T5KO mice resulted in microbiota components having elevated levels of bioactive lipopolysaccharide and flagellin, a modest level of low-grade inflammation and increased adiposity.
29617463	11	46	theme	flagellin	1969:1977	arg1	levels					1926:1931	elevated levels	1917:1931	elevated levels of bioactive lipopolysaccharide and flagellin	1917:1977	AIEC LF82 inoculation in ASF T5KO mice resulted in microbiota components having elevated levels of bioactive lipopolysaccharide and flagellin, a modest level of low-grade inflammation and increased adiposity.
29617463	13	47	theme	microbiota	2270:2279	arg1	composition					2281:2291	microbiota composition	2270:2291	microbiota composition	2270:2291	Addition of AIEC to this ecosystem perturbs microbiota composition, increases levels of lipopolysaccharide and flagellin, but only modestly promotes gut inflammation and adiposity, suggesting that the phenotypes previously associated with loss of this innate immune receptor require disruption of complex microbiota.
29617463	13	48	theme	innate	2478:2483	arg1	receptor					2492:2499	this innate immune receptor	2473:2499	this innate immune receptor	2473:2499	Addition of AIEC to this ecosystem perturbs microbiota composition, increases levels of lipopolysaccharide and flagellin, but only modestly promotes gut inflammation and adiposity, suggesting that the phenotypes previously associated with loss of this innate immune receptor require disruption of complex microbiota.
29617463	6	49	theme	WT	1080:1081	arg1	mice					1102:1105	WT and TLR5-deficient mice	1080:1105	WT and TLR5-deficient mice	1080:1105	For this purpose, WT and TLR5-deficient mice were generated and maintained with the 8-member consortium of bacteria referred to as "Altered Schaedler Flora" (ASF).
29617463	3	50	theme	TLR5-deficient	658:671	arg1	mice					673:676	TLR5-deficient mice	658:676	TLR5-deficient mice	658:676	That the complete absence of a microbiota (i.e. germfree conditions) eliminates all evidence of inflammation in TLR5-deficient mice demonstrates that this model of gut inflammation is microbiota-dependent.
29617463	13	51	theme	receptor	2492:2499	arg1	loss					2465:2468	loss	2465:2468	loss of this innate immune receptor	2465:2499	Addition of AIEC to this ecosystem perturbs microbiota composition, increases levels of lipopolysaccharide and flagellin, but only modestly promotes gut inflammation and adiposity, suggesting that the phenotypes previously associated with loss of this innate immune receptor require disruption of complex microbiota.
29617463	9	52	dep	display	1540:1546	arg1	Relative					1465:1472	Relative	1465:1472	Relative	1465:1472	RESULTS Relative to similarly maintained WT mice, mice lacking TLR5 (T5KO) did not display low-grade intestinal inflammation nor metabolic syndrome under ASF conditions.
29617463	10	53	theme	community	1782:1790	arg1	alteration					1760:1769	alteration	1760:1769	alteration of the ASF community in T5KO mice	1760:1803	Concomitantly, the ASF microbial community was similar between WT and T5KO mice, while inoculation with AIEC strain LF82 resulted in alteration of the ASF community in T5KO mice compared to WT control animals.
29617463	5	54	theme	pathobiont-free	1034:1048	arg1	microbiota					1050:1059	a limited and pathobiont-free microbiota	1020:1059	a limited and pathobiont-free microbiota	1020:1059	Herein, we examined the extent to which microbiota mismanagement and associated inflammation in TLR5-deficient mice would manifest in a limited and pathobiont-free microbiota.
29617463	11	55	theme	elevated	1917:1924	arg1	levels					1926:1931	elevated levels	1917:1931	elevated levels of bioactive lipopolysaccharide and flagellin	1917:1977	AIEC LF82 inoculation in ASF T5KO mice resulted in microbiota components having elevated levels of bioactive lipopolysaccharide and flagellin, a modest level of low-grade inflammation and increased adiposity.
29617463	3	56	dep	conditions	603:612	arg1	i.e.					589:592	i.e.	589:592	i.e.	589:592	That the complete absence of a microbiota (i.e. germfree conditions) eliminates all evidence of inflammation in TLR5-deficient mice demonstrates that this model of gut inflammation is microbiota-dependent.
29617463	12	57	theme	pathobiont-free	2082:2096	arg1	microbiota					2098:2107	a limited-complexity pathobiont-free microbiota	2061:2107	a limited-complexity pathobiont-free microbiota	2061:2107	CONCLUSIONS In a limited-complexity pathobiont-free microbiota, loss of the flagellin receptor TLR5 does not impact microbiota composition nor its ability to promote inflammation.
29617463	2	58	from	dysfunction	452:462	arg1	proteins					472:479	host proteins	467:479	host proteins	467:479	Alterations in microbiota, and associated inflammation, can originate from dysfunction in host proteins that manage the microbiota, such as the flagellin receptor TLR5.
29617463	2	58	from	dysfunction	452:462	arg1	TLR5					540:543	the flagellin receptor TLR5	517:543	the flagellin receptor TLR5	517:543	Alterations in microbiota, and associated inflammation, can originate from dysfunction in host proteins that manage the microbiota, such as the flagellin receptor TLR5.
29617463	6	59	theme	8-member	1146:1153	arg1	consortium					1155:1164	the 8-member consortium	1142:1164	the 8-member consortium of bacteria referred to as "Altered Schaedler Flora" (ASF)	1142:1223	For this purpose, WT and TLR5-deficient mice were generated and maintained with the 8-member consortium of bacteria referred to as "Altered Schaedler Flora" (ASF).
29617463	7	60	theme	AIEC	1277:1280	arg1	strain					1292:1297	AIEC reference strain LF82	1277:1302	AIEC reference strain LF82	1277:1302	Such ASF animals were subsequently inoculated with AIEC reference strain LF82.
29617463	3	61	theme	inflammation	714:725	arg1	model					701:705	this model	696:705	this model of gut inflammation	696:725	That the complete absence of a microbiota (i.e. germfree conditions) eliminates all evidence of inflammation in TLR5-deficient mice demonstrates that this model of gut inflammation is microbiota-dependent.
29617463	10	62	with	inoculation	1714:1724	arg1	strain					1736:1741	AIEC strain LF82	1731:1746	AIEC strain LF82	1731:1746	Concomitantly, the ASF microbial community was similar between WT and T5KO mice, while inoculation with AIEC strain LF82 resulted in alteration of the ASF community in T5KO mice compared to WT control animals.
29617463	9	63	theme	WT	1498:1499	arg1	mice					1501:1504	similarly maintained WT mice	1477:1504	similarly maintained WT mice	1477:1504	RESULTS Relative to similarly maintained WT mice, mice lacking TLR5 (T5KO) did not display low-grade intestinal inflammation nor metabolic syndrome under ASF conditions.
29617463	6	64	theme	Flora	1212:1216	arg1	"					1217:1217	"Altered Schaedler Flora"	1193:1217	"Altered Schaedler Flora" (ASF)	1193:1223	For this purpose, WT and TLR5-deficient mice were generated and maintained with the 8-member consortium of bacteria referred to as "Altered Schaedler Flora" (ASF).
29617463	6	64	theme	Flora	1212:1216	arg1	ASF					1220:1222	ASF	1220:1222	ASF	1220:1222	For this purpose, WT and TLR5-deficient mice were generated and maintained with the 8-member consortium of bacteria referred to as "Altered Schaedler Flora" (ASF).
29617463	10	65	theme	control	1820:1826	arg1	animals					1828:1834	WT control animals	1817:1834	WT control animals	1817:1834	Concomitantly, the ASF microbial community was similar between WT and T5KO mice, while inoculation with AIEC strain LF82 resulted in alteration of the ASF community in T5KO mice compared to WT control animals.
29617463	13	66	theme	gut	2375:2377	arg1	inflammation					2379:2390	gut inflammation	2375:2390	gut inflammation	2375:2390	Addition of AIEC to this ecosystem perturbs microbiota composition, increases levels of lipopolysaccharide and flagellin, but only modestly promotes gut inflammation and adiposity, suggesting that the phenotypes previously associated with loss of this innate immune receptor require disruption of complex microbiota.
29617463	6	67	theme	Altered	1194:1200	arg1	"					1217:1217	"Altered Schaedler Flora"	1193:1217	"Altered Schaedler Flora" (ASF)	1193:1223	For this purpose, WT and TLR5-deficient mice were generated and maintained with the 8-member consortium of bacteria referred to as "Altered Schaedler Flora" (ASF).
29617463	6	67	theme	Altered	1194:1200	arg1	ASF					1220:1222	ASF	1220:1222	ASF	1220:1222	For this purpose, WT and TLR5-deficient mice were generated and maintained with the 8-member consortium of bacteria referred to as "Altered Schaedler Flora" (ASF).
29617463	10	68	theme	ASF	1646:1648	arg1	similar					1674:1680	similar	1674:1680	similar	1674:1680	Concomitantly, the ASF microbial community was similar between WT and T5KO mice, while inoculation with AIEC strain LF82 resulted in alteration of the ASF community in T5KO mice compared to WT control animals.
29617463	10	68	theme	ASF	1646:1648	arg1	community					1660:1668	the ASF microbial community	1642:1668	the ASF microbial community	1642:1668	Concomitantly, the ASF microbial community was similar between WT and T5KO mice, while inoculation with AIEC strain LF82 resulted in alteration of the ASF community in T5KO mice compared to WT control animals.
29617463	11	69	theme	LF82	1842:1845	arg1	inoculation					1847:1857	AIEC LF82 inoculation	1837:1857	AIEC LF82 inoculation in ASF T5KO mice	1837:1874	AIEC LF82 inoculation in ASF T5KO mice resulted in microbiota components having elevated levels of bioactive lipopolysaccharide and flagellin, a modest level of low-grade inflammation and increased adiposity.
29617463	5	70	theme	microbiota	926:935	arg1	inflammation					966:977	microbiota mismanagement and associated inflammation	926:977	microbiota mismanagement and associated inflammation in TLR5-deficient mice	926:1000	Herein, we examined the extent to which microbiota mismanagement and associated inflammation in TLR5-deficient mice would manifest in a limited and pathobiont-free microbiota.
29617463	12	71	theme	microbiota	2162:2171	arg1	composition					2173:2183	microbiota composition	2162:2183	microbiota composition	2162:2183	CONCLUSIONS In a limited-complexity pathobiont-free microbiota, loss of the flagellin receptor TLR5 does not impact microbiota composition nor its ability to promote inflammation.
29617463	10	72	theme	WT	1690:1691	arg1	mice					1702:1705	WT and T5KO mice	1690:1705	WT and T5KO mice	1690:1705	Concomitantly, the ASF microbial community was similar between WT and T5KO mice, while inoculation with AIEC strain LF82 resulted in alteration of the ASF community in T5KO mice compared to WT control animals.
29617463	11	73	theme	low-grade	1998:2006	arg1	inflammation					2008:2019	low-grade inflammation	1998:2019	low-grade inflammation	1998:2019	AIEC LF82 inoculation in ASF T5KO mice resulted in microbiota components having elevated levels of bioactive lipopolysaccharide and flagellin, a modest level of low-grade inflammation and increased adiposity.
29617463	1	74	theme	chronic	224:230	arg1	inflammation					236:247	chronic gut inflammation	224:247	chronic gut inflammation	224:247	BACKGROUND Inability to maintain a stable and beneficial microbiota is associated with chronic gut inflammation, which classically manifests as colitis but may more commonly exist as low-grade inflammation that promotes metabolic syndrome.
29617463	11	75	theme	T5KO	1866:1869	arg1	mice					1871:1874	ASF T5KO mice	1862:1874	ASF T5KO mice	1862:1874	AIEC LF82 inoculation in ASF T5KO mice resulted in microbiota components having elevated levels of bioactive lipopolysaccharide and flagellin, a modest level of low-grade inflammation and increased adiposity.
29617463	3	76	theme	germfree	594:601	arg1	microbiota					577:586	a microbiota	575:586	a microbiota (i.e. germfree conditions)	575:613	That the complete absence of a microbiota (i.e. germfree conditions) eliminates all evidence of inflammation in TLR5-deficient mice demonstrates that this model of gut inflammation is microbiota-dependent.
29617463	3	76	theme	germfree	594:601	arg1	conditions					603:612	germfree conditions	594:612	germfree conditions	594:612	That the complete absence of a microbiota (i.e. germfree conditions) eliminates all evidence of inflammation in TLR5-deficient mice demonstrates that this model of gut inflammation is microbiota-dependent.
29617463	5	77	theme	mismanagement	937:949	arg1	inflammation					966:977	microbiota mismanagement and associated inflammation	926:977	microbiota mismanagement and associated inflammation in TLR5-deficient mice	926:1000	Herein, we examined the extent to which microbiota mismanagement and associated inflammation in TLR5-deficient mice would manifest in a limited and pathobiont-free microbiota.
29617463	12	78	theme	TLR5	2141:2144	arg1	loss					2110:2113	loss	2110:2113	loss of the flagellin receptor TLR5	2110:2144	CONCLUSIONS In a limited-complexity pathobiont-free microbiota, loss of the flagellin receptor TLR5 does not impact microbiota composition nor its ability to promote inflammation.
29617463	1	79	theme	BACKGROUND	137:146	arg1	Inability					148:156	BACKGROUND Inability	137:156	BACKGROUND Inability to maintain a stable and beneficial microbiota	137:203	BACKGROUND Inability to maintain a stable and beneficial microbiota is associated with chronic gut inflammation, which classically manifests as colitis but may more commonly exist as low-grade inflammation that promotes metabolic syndrome.
29617463	13	80	theme	microbiota	2531:2540	arg1	disruption					2509:2518	disruption	2509:2518	disruption of complex microbiota	2509:2540	Addition of AIEC to this ecosystem perturbs microbiota composition, increases levels of lipopolysaccharide and flagellin, but only modestly promotes gut inflammation and adiposity, suggesting that the phenotypes previously associated with loss of this innate immune receptor require disruption of complex microbiota.
29617463	8	81	theme	bacterial	1328:1336	arg1	loads					1338:1342	bacterial loads	1328:1342	bacterial loads	1328:1342	Feces were assayed for bacterial loads, fecal lipopolysaccharide and flagellin loads, fecal inflammatory marker lipocalin-2 and microbiota composition.
29617463	8	81	theme	bacterial	1328:1336	arg1	lipocalin-2					1417:1427	fecal inflammatory marker lipocalin-2	1391:1427	fecal inflammatory marker lipocalin-2	1391:1427	Feces were assayed for bacterial loads, fecal lipopolysaccharide and flagellin loads, fecal inflammatory marker lipocalin-2 and microbiota composition.
29617463	8	81	theme	bacterial	1328:1336	arg1	composition					1444:1454	microbiota composition	1433:1454	microbiota composition	1433:1454	Feces were assayed for bacterial loads, fecal lipopolysaccharide and flagellin loads, fecal inflammatory marker lipocalin-2 and microbiota composition.
29617463	9	82	theme	low-grade	1548:1556	arg1	inflammation					1569:1580	low-grade intestinal inflammation	1548:1580	low-grade intestinal inflammation	1548:1580	RESULTS Relative to similarly maintained WT mice, mice lacking TLR5 (T5KO) did not display low-grade intestinal inflammation nor metabolic syndrome under ASF conditions.
29617463	10	83	from	alteration	1760:1769	arg1	mice					1800:1803	T5KO mice	1795:1803	T5KO mice	1795:1803	Concomitantly, the ASF microbial community was similar between WT and T5KO mice, while inoculation with AIEC strain LF82 resulted in alteration of the ASF community in T5KO mice compared to WT control animals.
29617463	1	84	theme	metabolic	357:365	arg1	syndrome					367:374	metabolic syndrome	357:374	metabolic syndrome	357:374	BACKGROUND Inability to maintain a stable and beneficial microbiota is associated with chronic gut inflammation, which classically manifests as colitis but may more commonly exist as low-grade inflammation that promotes metabolic syndrome.
29617463	12	85	theme	flagellin	2122:2130	arg1	TLR5					2141:2144	the flagellin receptor TLR5	2118:2144	the flagellin receptor TLR5	2118:2144	CONCLUSIONS In a limited-complexity pathobiont-free microbiota, loss of the flagellin receptor TLR5 does not impact microbiota composition nor its ability to promote inflammation.
29617463	7	86	theme	Such	1226:1229	arg1	animals					1235:1241	Such ASF animals	1226:1241	Such ASF animals	1226:1241	Such ASF animals were subsequently inoculated with AIEC reference strain LF82.
29617463	9	87	theme	metabolic	1586:1594	arg1	syndrome					1596:1603	metabolic syndrome	1586:1603	metabolic syndrome	1586:1603	RESULTS Relative to similarly maintained WT mice, mice lacking TLR5 (T5KO) did not display low-grade intestinal inflammation nor metabolic syndrome under ASF conditions.
29617463	1	88	dep	Inability	148:156	arg1	maintain					161:168	maintain	161:168	to maintain a stable and beneficial microbiota	158:203	BACKGROUND Inability to maintain a stable and beneficial microbiota is associated with chronic gut inflammation, which classically manifests as colitis but may more commonly exist as low-grade inflammation that promotes metabolic syndrome.
29617463	8	89	theme	flagellin	1374:1382	arg1	loads					1384:1388	flagellin loads	1374:1388	flagellin loads	1374:1388	Feces were assayed for bacterial loads, fecal lipopolysaccharide and flagellin loads, fecal inflammatory marker lipocalin-2 and microbiota composition.
29617463	0	90	theme	immune	118:123	arg1	deficiency					125:134	an innate immune deficiency	108:134	an innate immune deficiency	108:134	Mice harboring pathobiont-free microbiota do not develop intestinal inflammation that normally results from an innate immune deficiency.
29617463	2	91	theme	receptor	531:538	arg1	TLR5					540:543	the flagellin receptor TLR5	517:543	the flagellin receptor TLR5	517:543	Alterations in microbiota, and associated inflammation, can originate from dysfunction in host proteins that manage the microbiota, such as the flagellin receptor TLR5.
29617463	5	92	from	inflammation	966:977	arg1	mice					997:1000	TLR5-deficient mice	982:1000	TLR5-deficient mice	982:1000	Herein, we examined the extent to which microbiota mismanagement and associated inflammation in TLR5-deficient mice would manifest in a limited and pathobiont-free microbiota.
29617463	4	93	theme	such	772:775	arg1	dependency					788:797	such microbiota dependency	772:797	such microbiota dependency	772:797	We hypothesize that such microbiota dependency reflects an inability to manage pathobionts, such as Adherent-Invasive E. coli (AIEC).
29617463	11	94	theme	bioactive	1936:1944	arg1	lipopolysaccharide					1946:1963	bioactive lipopolysaccharide	1936:1963	bioactive lipopolysaccharide	1936:1963	AIEC LF82 inoculation in ASF T5KO mice resulted in microbiota components having elevated levels of bioactive lipopolysaccharide and flagellin, a modest level of low-grade inflammation and increased adiposity.
29617463	11	95	contain	having	1910:1915	arg2	levels					1926:1931	elevated levels	1917:1931	elevated levels of bioactive lipopolysaccharide and flagellin	1917:1977	AIEC LF82 inoculation in ASF T5KO mice resulted in microbiota components having elevated levels of bioactive lipopolysaccharide and flagellin, a modest level of low-grade inflammation and increased adiposity.
29617463	11	95	contain	having	1910:1915	arg2	level					1989:1993	a modest level	1980:1993	a modest level of low-grade inflammation	1980:2019	AIEC LF82 inoculation in ASF T5KO mice resulted in microbiota components having elevated levels of bioactive lipopolysaccharide and flagellin, a modest level of low-grade inflammation and increased adiposity.
29617463	11	95	contain	having	1910:1915	arg2	adiposity					2035:2043	increased adiposity	2025:2043	increased adiposity	2025:2043	AIEC LF82 inoculation in ASF T5KO mice resulted in microbiota components having elevated levels of bioactive lipopolysaccharide and flagellin, a modest level of low-grade inflammation and increased adiposity.
29617463	11	95	contain	having	1910:1915	arg1	components					1899:1908	microbiota components	1888:1908	microbiota components having elevated levels of bioactive lipopolysaccharide and flagellin, a modest level of low-grade inflammation and increased adiposity	1888:2043	AIEC LF82 inoculation in ASF T5KO mice resulted in microbiota components having elevated levels of bioactive lipopolysaccharide and flagellin, a modest level of low-grade inflammation and increased adiposity.
29617463	10	96	dep	resulted	1748:1755	arg1	compared					1805:1812	compared	1805:1812	compared to WT control animals	1805:1834	Concomitantly, the ASF microbial community was similar between WT and T5KO mice, while inoculation with AIEC strain LF82 resulted in alteration of the ASF community in T5KO mice compared to WT control animals.
29617463	10	97	theme	ASF	1778:1780	arg1	community					1782:1790	the ASF community	1774:1790	the ASF community	1774:1790	Concomitantly, the ASF microbial community was similar between WT and T5KO mice, while inoculation with AIEC strain LF82 resulted in alteration of the ASF community in T5KO mice compared to WT control animals.
29617463	12	98	dep	CONCLUSIONS	2046:2056	arg1	microbiota					2098:2107	a limited-complexity pathobiont-free microbiota	2061:2107	a limited-complexity pathobiont-free microbiota	2061:2107	CONCLUSIONS In a limited-complexity pathobiont-free microbiota, loss of the flagellin receptor TLR5 does not impact microbiota composition nor its ability to promote inflammation.
29617463	8	99	theme	microbiota	1433:1442	arg1	loads					1338:1342	bacterial loads	1328:1342	bacterial loads	1328:1342	Feces were assayed for bacterial loads, fecal lipopolysaccharide and flagellin loads, fecal inflammatory marker lipocalin-2 and microbiota composition.
29617463	8	99	theme	microbiota	1433:1442	arg1	composition					1444:1454	microbiota composition	1433:1454	microbiota composition	1433:1454	Feces were assayed for bacterial loads, fecal lipopolysaccharide and flagellin loads, fecal inflammatory marker lipocalin-2 and microbiota composition.
29617463	0	100	theme	intestinal	57:66	arg1	inflammation					68:79	intestinal inflammation	57:79	intestinal inflammation that normally results from an innate immune deficiency	57:134	Mice harboring pathobiont-free microbiota do not develop intestinal inflammation that normally results from an innate immune deficiency.
29617463	1	101	theme	low-grade	320:328	arg1	inflammation					330:341	low-grade inflammation	320:341	low-grade inflammation that promotes metabolic syndrome	320:374	BACKGROUND Inability to maintain a stable and beneficial microbiota is associated with chronic gut inflammation, which classically manifests as colitis but may more commonly exist as low-grade inflammation that promotes metabolic syndrome.
29617463	13	102	theme	immune	2485:2490	arg1	receptor					2492:2499	this innate immune receptor	2473:2499	this innate immune receptor	2473:2499	Addition of AIEC to this ecosystem perturbs microbiota composition, increases levels of lipopolysaccharide and flagellin, but only modestly promotes gut inflammation and adiposity, suggesting that the phenotypes previously associated with loss of this innate immune receptor require disruption of complex microbiota.
29617463	11	103	theme	microbiota	1888:1897	arg1	components					1899:1908	microbiota components	1888:1908	microbiota components having elevated levels of bioactive lipopolysaccharide and flagellin, a modest level of low-grade inflammation and increased adiposity	1888:2043	AIEC LF82 inoculation in ASF T5KO mice resulted in microbiota components having elevated levels of bioactive lipopolysaccharide and flagellin, a modest level of low-grade inflammation and increased adiposity.
29617463	2	104	from	inflammation	419:430	arg1	microbiota					392:401	microbiota	392:401	microbiota	392:401	Alterations in microbiota, and associated inflammation, can originate from dysfunction in host proteins that manage the microbiota, such as the flagellin receptor TLR5.
29617463	6	105	theme	bacteria	1169:1176	arg1	consortium					1155:1164	the 8-member consortium	1142:1164	the 8-member consortium of bacteria referred to as "Altered Schaedler Flora" (ASF)	1142:1223	For this purpose, WT and TLR5-deficient mice were generated and maintained with the 8-member consortium of bacteria referred to as "Altered Schaedler Flora" (ASF).
29617463	5	106	theme	limited	1022:1028	arg1	microbiota					1050:1059	a limited and pathobiont-free microbiota	1020:1059	a limited and pathobiont-free microbiota	1020:1059	Herein, we examined the extent to which microbiota mismanagement and associated inflammation in TLR5-deficient mice would manifest in a limited and pathobiont-free microbiota.
29617463	7	107	theme	reference	1282:1290	arg1	strain					1292:1297	AIEC reference strain LF82	1277:1302	AIEC reference strain LF82	1277:1302	Such ASF animals were subsequently inoculated with AIEC reference strain LF82.
31660562	2	0	theme	atom	586:589	arg1	SI-ATRP					624:630	SI-ATRP	624:630	SI-ATRP	624:630	Large amounts of graphene quantum dots (GQDs) were embedded in the γ-CD-MOF matrix (denoted as GQDs@γ-CD-MOF) to endow the γ-CD-MOF with strong fluorescence, which was then modified by pH responsive poly(ethyleneglycol)dimethacrylate (PEGMA) through surface initiated atom transfer radical polymerization (SI-ATRP) to fabricate the PEGMA@GQDs@γ-CD-MOF composite.
31660562	2	0	theme	atom	586:589	arg1	polymerization					608:621	surface initiated atom transfer radical polymerization	568:621	surface initiated atom transfer radical polymerization (SI-ATRP)	568:631	Large amounts of graphene quantum dots (GQDs) were embedded in the γ-CD-MOF matrix (denoted as GQDs@γ-CD-MOF) to endow the γ-CD-MOF with strong fluorescence, which was then modified by pH responsive poly(ethyleneglycol)dimethacrylate (PEGMA) through surface initiated atom transfer radical polymerization (SI-ATRP) to fabricate the PEGMA@GQDs@γ-CD-MOF composite.
31660562	1	1	theme	drug	284:287	arg1	delivery					289:296	targeted anticancer drug delivery	264:296	targeted anticancer drug delivery	264:296	The γ-cyclodextrin-based metal-organic framework (γ-CD-MOF) composite was designed and prepared toward targeted anticancer drug delivery and cancer therapy.
31660562	2	2	theme	@	655:655	arg1	composite					670:678	the PEGMA@GQDs@γ-CD-MOF composite	646:678	the PEGMA@GQDs@γ-CD-MOF composite	646:678	Large amounts of graphene quantum dots (GQDs) were embedded in the γ-CD-MOF matrix (denoted as GQDs@γ-CD-MOF) to endow the γ-CD-MOF with strong fluorescence, which was then modified by pH responsive poly(ethyleneglycol)dimethacrylate (PEGMA) through surface initiated atom transfer radical polymerization (SI-ATRP) to fabricate the PEGMA@GQDs@γ-CD-MOF composite.
31660562	2	3	theme	surface	568:574	arg1	SI-ATRP					624:630	SI-ATRP	624:630	SI-ATRP	624:630	Large amounts of graphene quantum dots (GQDs) were embedded in the γ-CD-MOF matrix (denoted as GQDs@γ-CD-MOF) to endow the γ-CD-MOF with strong fluorescence, which was then modified by pH responsive poly(ethyleneglycol)dimethacrylate (PEGMA) through surface initiated atom transfer radical polymerization (SI-ATRP) to fabricate the PEGMA@GQDs@γ-CD-MOF composite.
31660562	2	3	theme	surface	568:574	arg1	polymerization					608:621	surface initiated atom transfer radical polymerization	568:621	surface initiated atom transfer radical polymerization (SI-ATRP)	568:631	Large amounts of graphene quantum dots (GQDs) were embedded in the γ-CD-MOF matrix (denoted as GQDs@γ-CD-MOF) to endow the γ-CD-MOF with strong fluorescence, which was then modified by pH responsive poly(ethyleneglycol)dimethacrylate (PEGMA) through surface initiated atom transfer radical polymerization (SI-ATRP) to fabricate the PEGMA@GQDs@γ-CD-MOF composite.
31660562	5	4	theme	mediated	1371:1378	arg1	endocytosis					1380:1390	receptor mediated endocytosis	1362:1390	receptor mediated endocytosis with high selectivity	1362:1412	The targeting specificity investigation revealed that this DOX delivery system was effectively internalized via receptor mediated endocytosis with high selectivity.
31660562	6	5	theme	tumour	1490:1495	arg1	growth					1497:1502	the tumour growth	1486:1502	the tumour growth	1486:1502	The in vivo antitumour study with tumour-bearing mice illustrated that the tumour growth can be effectively suppressed and partially ablated with negligible side effects after treatments.
31660562	7	6	theme	PEGMA	1634:1638	arg1	composite					1653:1661	the proposed AS1411@PEGMA@GQD@γ-CD-MOF composite	1614:1661	the proposed AS1411@PEGMA@GQD@γ-CD-MOF composite	1614:1661	Therefore, the proposed AS1411@PEGMA@GQD@γ-CD-MOF composite is promising for effective DOX delivery and tumour growth inhibition both in vitro and in vivo, showing great potential for anticancer therapy.
31660562	7	6	theme	PEGMA	1634:1638	arg1	promising					1666:1674	promising	1666:1674	promising	1666:1674	Therefore, the proposed AS1411@PEGMA@GQD@γ-CD-MOF composite is promising for effective DOX delivery and tumour growth inhibition both in vitro and in vivo, showing great potential for anticancer therapy.
31660562	4	7	theme	pH-responsive	1063:1075	arg1	system					1090:1095	the resultant pH-responsive DOX delivery system	1049:1095	the resultant pH-responsive DOX delivery system (denoted as DOX/AS1411@PEGMA@GQDs@γ-CD-MOF)	1049:1139	Large amounts of antitumour drug, doxorubicin hydrochloride (DOX), could be encapsulated within this composite due to the chemical-rich functionality, and the resultant pH-responsive DOX delivery system (denoted as DOX/AS1411@PEGMA@GQDs@γ-CD-MOF) displayed a higher DOX loading of 89.1% with sustained release than the pristine γ-CD-MOF and GQDs@γ-CD-MOF.
31660562	4	8	theme	GQDs	1235:1238	arg1	γ-CD-MOF					1240:1247	the pristine γ-CD-MOF and GQDs@γ-CD-MOF	1209:1247	γ-CD-MOF	1240:1247	Large amounts of antitumour drug, doxorubicin hydrochloride (DOX), could be encapsulated within this composite due to the chemical-rich functionality, and the resultant pH-responsive DOX delivery system (denoted as DOX/AS1411@PEGMA@GQDs@γ-CD-MOF) displayed a higher DOX loading of 89.1% with sustained release than the pristine γ-CD-MOF and GQDs@γ-CD-MOF.
31660562	1	9	theme	framework	200:208	arg1	composite					221:229	The γ-cyclodextrin-based metal-organic framework (γ-CD-MOF) composite	161:229	The γ-cyclodextrin-based metal-organic framework (γ-CD-MOF) composite	161:229	The γ-cyclodextrin-based metal-organic framework (γ-CD-MOF) composite was designed and prepared toward targeted anticancer drug delivery and cancer therapy.
31660562	4	10	with	loading	1164:1170	arg1	release					1196:1202	sustained release	1186:1202	sustained release	1186:1202	Large amounts of antitumour drug, doxorubicin hydrochloride (DOX), could be encapsulated within this composite due to the chemical-rich functionality, and the resultant pH-responsive DOX delivery system (denoted as DOX/AS1411@PEGMA@GQDs@γ-CD-MOF) displayed a higher DOX loading of 89.1% with sustained release than the pristine γ-CD-MOF and GQDs@γ-CD-MOF.
31660562	2	11	theme	radical	600:606	arg1	SI-ATRP					624:630	SI-ATRP	624:630	SI-ATRP	624:630	Large amounts of graphene quantum dots (GQDs) were embedded in the γ-CD-MOF matrix (denoted as GQDs@γ-CD-MOF) to endow the γ-CD-MOF with strong fluorescence, which was then modified by pH responsive poly(ethyleneglycol)dimethacrylate (PEGMA) through surface initiated atom transfer radical polymerization (SI-ATRP) to fabricate the PEGMA@GQDs@γ-CD-MOF composite.
31660562	2	11	theme	radical	600:606	arg1	polymerization					608:621	surface initiated atom transfer radical polymerization	568:621	surface initiated atom transfer radical polymerization (SI-ATRP)	568:631	Large amounts of graphene quantum dots (GQDs) were embedded in the γ-CD-MOF matrix (denoted as GQDs@γ-CD-MOF) to endow the γ-CD-MOF with strong fluorescence, which was then modified by pH responsive poly(ethyleneglycol)dimethacrylate (PEGMA) through surface initiated atom transfer radical polymerization (SI-ATRP) to fabricate the PEGMA@GQDs@γ-CD-MOF composite.
31660562	7	12	theme	growth	1714:1719	arg1	inhibition					1721:1730	tumour growth inhibition	1707:1730	tumour growth inhibition	1707:1730	Therefore, the proposed AS1411@PEGMA@GQD@γ-CD-MOF composite is promising for effective DOX delivery and tumour growth inhibition both in vitro and in vivo, showing great potential for anticancer therapy.
31660562	4	13	theme	delivery	1081:1088	arg1	system					1090:1095	the resultant pH-responsive DOX delivery system	1049:1095	the resultant pH-responsive DOX delivery system (denoted as DOX/AS1411@PEGMA@GQDs@γ-CD-MOF)	1049:1139	Large amounts of antitumour drug, doxorubicin hydrochloride (DOX), could be encapsulated within this composite due to the chemical-rich functionality, and the resultant pH-responsive DOX delivery system (denoted as DOX/AS1411@PEGMA@GQDs@γ-CD-MOF) displayed a higher DOX loading of 89.1% with sustained release than the pristine γ-CD-MOF and GQDs@γ-CD-MOF.
31660562	2	14	theme	graphene	335:342	arg1	GQDs					358:361	GQDs	358:361	GQDs	358:361	Large amounts of graphene quantum dots (GQDs) were embedded in the γ-CD-MOF matrix (denoted as GQDs@γ-CD-MOF) to endow the γ-CD-MOF with strong fluorescence, which was then modified by pH responsive poly(ethyleneglycol)dimethacrylate (PEGMA) through surface initiated atom transfer radical polymerization (SI-ATRP) to fabricate the PEGMA@GQDs@γ-CD-MOF composite.
31660562	2	14	theme	graphene	335:342	arg1	dots					352:355	graphene quantum dots	335:355	graphene quantum dots (GQDs)	335:362	Large amounts of graphene quantum dots (GQDs) were embedded in the γ-CD-MOF matrix (denoted as GQDs@γ-CD-MOF) to endow the γ-CD-MOF with strong fluorescence, which was then modified by pH responsive poly(ethyleneglycol)dimethacrylate (PEGMA) through surface initiated atom transfer radical polymerization (SI-ATRP) to fabricate the PEGMA@GQDs@γ-CD-MOF composite.
31660562	1	15	theme	γ-CD-MOF	211:218	arg1	composite					221:229	The γ-cyclodextrin-based metal-organic framework (γ-CD-MOF) composite	161:229	The γ-cyclodextrin-based metal-organic framework (γ-CD-MOF) composite	161:229	The γ-cyclodextrin-based metal-organic framework (γ-CD-MOF) composite was designed and prepared toward targeted anticancer drug delivery and cancer therapy.
31660562	3	16	theme	cell-targeted	698:710	arg1	probe					712:716	the cancer cell-targeted probe	687:716	the cancer cell-targeted probe	687:716	Then, the cancer cell-targeted probe was obtained by immobilizing the AS1411 aptamer over it (denoted as AS1411@PEGMA@GQDs@γ-CD-MOF) and it exhibits pH-responsive release function and excellent targeting ability.
31660562	0	17	theme	anticancer	123:132	arg1	delivery					139:146	anticancer drug delivery	123:146	anticancer drug delivery	123:146	A γ-cyclodextrin-based metal-organic framework embedded with graphene quantum dots and modified with PEGMA via SI-ATRP for anticancer drug delivery and therapy.
31660562	7	18	theme	proposed	1618:1625	arg1	composite					1653:1661	the proposed AS1411@PEGMA@GQD@γ-CD-MOF composite	1614:1661	the proposed AS1411@PEGMA@GQD@γ-CD-MOF composite	1614:1661	Therefore, the proposed AS1411@PEGMA@GQD@γ-CD-MOF composite is promising for effective DOX delivery and tumour growth inhibition both in vitro and in vivo, showing great potential for anticancer therapy.
31660562	7	18	theme	proposed	1618:1625	arg1	promising					1666:1674	promising	1666:1674	promising	1666:1674	Therefore, the proposed AS1411@PEGMA@GQD@γ-CD-MOF composite is promising for effective DOX delivery and tumour growth inhibition both in vitro and in vivo, showing great potential for anticancer therapy.
31660562	7	19	theme	effective	1680:1688	arg1	delivery					1694:1701	effective DOX delivery	1680:1701	effective DOX delivery	1680:1701	Therefore, the proposed AS1411@PEGMA@GQD@γ-CD-MOF composite is promising for effective DOX delivery and tumour growth inhibition both in vitro and in vivo, showing great potential for anticancer therapy.
31660562	2	20	theme	γ-CD-MOF	661:668	arg1	composite					670:678	the PEGMA@GQDs@γ-CD-MOF composite	646:678	the PEGMA@GQDs@γ-CD-MOF composite	646:678	Large amounts of graphene quantum dots (GQDs) were embedded in the γ-CD-MOF matrix (denoted as GQDs@γ-CD-MOF) to endow the γ-CD-MOF with strong fluorescence, which was then modified by pH responsive poly(ethyleneglycol)dimethacrylate (PEGMA) through surface initiated atom transfer radical polymerization (SI-ATRP) to fabricate the PEGMA@GQDs@γ-CD-MOF composite.
31660562	4	21	theme	@	1119:1119	arg1	γ-CD-MOF					1131:1138	DOX/AS1411@PEGMA@GQDs@γ-CD-MOF	1109:1138	DOX/AS1411@PEGMA@GQDs@γ-CD-MOF	1109:1138	Large amounts of antitumour drug, doxorubicin hydrochloride (DOX), could be encapsulated within this composite due to the chemical-rich functionality, and the resultant pH-responsive DOX delivery system (denoted as DOX/AS1411@PEGMA@GQDs@γ-CD-MOF) displayed a higher DOX loading of 89.1% with sustained release than the pristine γ-CD-MOF and GQDs@γ-CD-MOF.
31660562	6	22	with	study	1438:1442	arg1	mice					1464:1467	tumour-bearing mice	1449:1467	tumour-bearing mice	1449:1467	The in vivo antitumour study with tumour-bearing mice illustrated that the tumour growth can be effectively suppressed and partially ablated with negligible side effects after treatments.
31660562	3	23	theme	GQDs	799:802	arg1	γ-CD-MOF					804:811	AS1411@PEGMA@GQDs@γ-CD-MOF	786:811	AS1411@PEGMA@GQDs@γ-CD-MOF	786:811	Then, the cancer cell-targeted probe was obtained by immobilizing the AS1411 aptamer over it (denoted as AS1411@PEGMA@GQDs@γ-CD-MOF) and it exhibits pH-responsive release function and excellent targeting ability.
31660562	2	24	theme	@	660:660	arg1	composite					670:678	the PEGMA@GQDs@γ-CD-MOF composite	646:678	the PEGMA@GQDs@γ-CD-MOF composite	646:678	Large amounts of graphene quantum dots (GQDs) were embedded in the γ-CD-MOF matrix (denoted as GQDs@γ-CD-MOF) to endow the γ-CD-MOF with strong fluorescence, which was then modified by pH responsive poly(ethyleneglycol)dimethacrylate (PEGMA) through surface initiated atom transfer radical polymerization (SI-ATRP) to fabricate the PEGMA@GQDs@γ-CD-MOF composite.
31660562	4	25	theme	@	1125:1125	arg1	γ-CD-MOF					1131:1138	DOX/AS1411@PEGMA@GQDs@γ-CD-MOF	1109:1138	DOX/AS1411@PEGMA@GQDs@γ-CD-MOF	1109:1138	Large amounts of antitumour drug, doxorubicin hydrochloride (DOX), could be encapsulated within this composite due to the chemical-rich functionality, and the resultant pH-responsive DOX delivery system (denoted as DOX/AS1411@PEGMA@GQDs@γ-CD-MOF) displayed a higher DOX loading of 89.1% with sustained release than the pristine γ-CD-MOF and GQDs@γ-CD-MOF.
31660562	4	26	dep	system	1090:1095	arg1	denoted					1098:1104	denoted	1098:1104	denoted as DOX/AS1411@PEGMA@GQDs@γ-CD-MOF	1098:1138	Large amounts of antitumour drug, doxorubicin hydrochloride (DOX), could be encapsulated within this composite due to the chemical-rich functionality, and the resultant pH-responsive DOX delivery system (denoted as DOX/AS1411@PEGMA@GQDs@γ-CD-MOF) displayed a higher DOX loading of 89.1% with sustained release than the pristine γ-CD-MOF and GQDs@γ-CD-MOF.
31660562	3	27	theme	PEGMA	793:797	arg1	γ-CD-MOF					804:811	AS1411@PEGMA@GQDs@γ-CD-MOF	786:811	AS1411@PEGMA@GQDs@γ-CD-MOF	786:811	Then, the cancer cell-targeted probe was obtained by immobilizing the AS1411 aptamer over it (denoted as AS1411@PEGMA@GQDs@γ-CD-MOF) and it exhibits pH-responsive release function and excellent targeting ability.
31660562	4	28	theme	drug	922:925	arg1	amounts					900:906	Large amounts	894:906	Large amounts of antitumour drug, doxorubicin hydrochloride (DOX),	894:959	Large amounts of antitumour drug, doxorubicin hydrochloride (DOX), could be encapsulated within this composite due to the chemical-rich functionality, and the resultant pH-responsive DOX delivery system (denoted as DOX/AS1411@PEGMA@GQDs@γ-CD-MOF) displayed a higher DOX loading of 89.1% with sustained release than the pristine γ-CD-MOF and GQDs@γ-CD-MOF.
31660562	4	28	theme	drug	922:925	arg1	drug					922:925	antitumour drug	911:925	antitumour drug	911:925	Large amounts of antitumour drug, doxorubicin hydrochloride (DOX), could be encapsulated within this composite due to the chemical-rich functionality, and the resultant pH-responsive DOX delivery system (denoted as DOX/AS1411@PEGMA@GQDs@γ-CD-MOF) displayed a higher DOX loading of 89.1% with sustained release than the pristine γ-CD-MOF and GQDs@γ-CD-MOF.
31660562	4	28	theme	drug	922:925	arg1	hydrochloride					940:952	doxorubicin hydrochloride	928:952	doxorubicin hydrochloride (DOX)	928:958	Large amounts of antitumour drug, doxorubicin hydrochloride (DOX), could be encapsulated within this composite due to the chemical-rich functionality, and the resultant pH-responsive DOX delivery system (denoted as DOX/AS1411@PEGMA@GQDs@γ-CD-MOF) displayed a higher DOX loading of 89.1% with sustained release than the pristine γ-CD-MOF and GQDs@γ-CD-MOF.
31660562	7	29	theme	DOX	1690:1692	arg1	delivery					1694:1701	effective DOX delivery	1680:1701	effective DOX delivery	1680:1701	Therefore, the proposed AS1411@PEGMA@GQD@γ-CD-MOF composite is promising for effective DOX delivery and tumour growth inhibition both in vitro and in vivo, showing great potential for anticancer therapy.
31660562	4	30	theme	doxorubicin	928:938	arg1	DOX					955:957	DOX	955:957	DOX	955:957	Large amounts of antitumour drug, doxorubicin hydrochloride (DOX), could be encapsulated within this composite due to the chemical-rich functionality, and the resultant pH-responsive DOX delivery system (denoted as DOX/AS1411@PEGMA@GQDs@γ-CD-MOF) displayed a higher DOX loading of 89.1% with sustained release than the pristine γ-CD-MOF and GQDs@γ-CD-MOF.
31660562	4	30	theme	doxorubicin	928:938	arg1	drug					922:925	antitumour drug	911:925	antitumour drug	911:925	Large amounts of antitumour drug, doxorubicin hydrochloride (DOX), could be encapsulated within this composite due to the chemical-rich functionality, and the resultant pH-responsive DOX delivery system (denoted as DOX/AS1411@PEGMA@GQDs@γ-CD-MOF) displayed a higher DOX loading of 89.1% with sustained release than the pristine γ-CD-MOF and GQDs@γ-CD-MOF.
31660562	4	30	theme	doxorubicin	928:938	arg1	hydrochloride					940:952	doxorubicin hydrochloride	928:952	doxorubicin hydrochloride (DOX)	928:958	Large amounts of antitumour drug, doxorubicin hydrochloride (DOX), could be encapsulated within this composite due to the chemical-rich functionality, and the resultant pH-responsive DOX delivery system (denoted as DOX/AS1411@PEGMA@GQDs@γ-CD-MOF) displayed a higher DOX loading of 89.1% with sustained release than the pristine γ-CD-MOF and GQDs@γ-CD-MOF.
31660562	3	31	theme	AS1411	786:791	arg1	γ-CD-MOF					804:811	AS1411@PEGMA@GQDs@γ-CD-MOF	786:811	AS1411@PEGMA@GQDs@γ-CD-MOF	786:811	Then, the cancer cell-targeted probe was obtained by immobilizing the AS1411 aptamer over it (denoted as AS1411@PEGMA@GQDs@γ-CD-MOF) and it exhibits pH-responsive release function and excellent targeting ability.
31660562	1	32	theme	γ-cyclodextrin-based	165:184	arg1	composite					221:229	The γ-cyclodextrin-based metal-organic framework (γ-CD-MOF) composite	161:229	The γ-cyclodextrin-based metal-organic framework (γ-CD-MOF) composite	161:229	The γ-cyclodextrin-based metal-organic framework (γ-CD-MOF) composite was designed and prepared toward targeted anticancer drug delivery and cancer therapy.
31660562	7	33	theme	@	1643:1643	arg1	composite					1653:1661	the proposed AS1411@PEGMA@GQD@γ-CD-MOF composite	1614:1661	the proposed AS1411@PEGMA@GQD@γ-CD-MOF composite	1614:1661	Therefore, the proposed AS1411@PEGMA@GQD@γ-CD-MOF composite is promising for effective DOX delivery and tumour growth inhibition both in vitro and in vivo, showing great potential for anticancer therapy.
31660562	7	33	theme	@	1643:1643	arg1	promising					1666:1674	promising	1666:1674	promising	1666:1674	Therefore, the proposed AS1411@PEGMA@GQD@γ-CD-MOF composite is promising for effective DOX delivery and tumour growth inhibition both in vitro and in vivo, showing great potential for anticancer therapy.
31660562	2	34	theme	responsive	506:515	arg1	PEGMA					553:557	PEGMA	553:557	PEGMA	553:557	Large amounts of graphene quantum dots (GQDs) were embedded in the γ-CD-MOF matrix (denoted as GQDs@γ-CD-MOF) to endow the γ-CD-MOF with strong fluorescence, which was then modified by pH responsive poly(ethyleneglycol)dimethacrylate (PEGMA) through surface initiated atom transfer radical polymerization (SI-ATRP) to fabricate the PEGMA@GQDs@γ-CD-MOF composite.
31660562	2	34	theme	responsive	506:515	arg1	dimethacrylate					537:550	pH responsive poly(ethyleneglycol)dimethacrylate	503:550	pH responsive poly(ethyleneglycol)dimethacrylate (PEGMA)	503:558	Large amounts of graphene quantum dots (GQDs) were embedded in the γ-CD-MOF matrix (denoted as GQDs@γ-CD-MOF) to endow the γ-CD-MOF with strong fluorescence, which was then modified by pH responsive poly(ethyleneglycol)dimethacrylate (PEGMA) through surface initiated atom transfer radical polymerization (SI-ATRP) to fabricate the PEGMA@GQDs@γ-CD-MOF composite.
31660562	3	35	theme	release	844:850	arg1	function					852:859	pH-responsive release function	830:859	pH-responsive release function	830:859	Then, the cancer cell-targeted probe was obtained by immobilizing the AS1411 aptamer over it (denoted as AS1411@PEGMA@GQDs@γ-CD-MOF) and it exhibits pH-responsive release function and excellent targeting ability.
31660562	4	36	theme	DOX	1160:1162	arg1	loading					1164:1170	a higher DOX loading	1151:1170	a higher DOX loading of 89.1% with sustained release	1151:1202	Large amounts of antitumour drug, doxorubicin hydrochloride (DOX), could be encapsulated within this composite due to the chemical-rich functionality, and the resultant pH-responsive DOX delivery system (denoted as DOX/AS1411@PEGMA@GQDs@γ-CD-MOF) displayed a higher DOX loading of 89.1% with sustained release than the pristine γ-CD-MOF and GQDs@γ-CD-MOF.
31660562	7	37	theme	@	1633:1633	arg1	composite					1653:1661	the proposed AS1411@PEGMA@GQD@γ-CD-MOF composite	1614:1661	the proposed AS1411@PEGMA@GQD@γ-CD-MOF composite	1614:1661	Therefore, the proposed AS1411@PEGMA@GQD@γ-CD-MOF composite is promising for effective DOX delivery and tumour growth inhibition both in vitro and in vivo, showing great potential for anticancer therapy.
31660562	7	37	theme	@	1633:1633	arg1	promising					1666:1674	promising	1666:1674	promising	1666:1674	Therefore, the proposed AS1411@PEGMA@GQD@γ-CD-MOF composite is promising for effective DOX delivery and tumour growth inhibition both in vitro and in vivo, showing great potential for anticancer therapy.
31660562	4	38	theme	@	1130:1130	arg1	γ-CD-MOF					1131:1138	DOX/AS1411@PEGMA@GQDs@γ-CD-MOF	1109:1138	DOX/AS1411@PEGMA@GQDs@γ-CD-MOF	1109:1138	Large amounts of antitumour drug, doxorubicin hydrochloride (DOX), could be encapsulated within this composite due to the chemical-rich functionality, and the resultant pH-responsive DOX delivery system (denoted as DOX/AS1411@PEGMA@GQDs@γ-CD-MOF) displayed a higher DOX loading of 89.1% with sustained release than the pristine γ-CD-MOF and GQDs@γ-CD-MOF.
31660562	3	39	theme	cancer	691:696	arg1	probe					712:716	the cancer cell-targeted probe	687:716	the cancer cell-targeted probe	687:716	Then, the cancer cell-targeted probe was obtained by immobilizing the AS1411 aptamer over it (denoted as AS1411@PEGMA@GQDs@γ-CD-MOF) and it exhibits pH-responsive release function and excellent targeting ability.
31660562	1	40	theme	cancer	302:307	arg1	therapy					309:315	cancer therapy	302:315	cancer therapy	302:315	The γ-cyclodextrin-based metal-organic framework (γ-CD-MOF) composite was designed and prepared toward targeted anticancer drug delivery and cancer therapy.
31660562	6	41	theme	negligible	1561:1570	arg1	effects					1577:1583	negligible side effects	1561:1583	negligible side effects after treatments	1561:1600	The in vivo antitumour study with tumour-bearing mice illustrated that the tumour growth can be effectively suppressed and partially ablated with negligible side effects after treatments.
31660562	3	42	theme	targeting	875:883	arg1	ability					885:891	excellent targeting ability	865:891	excellent targeting ability	865:891	Then, the cancer cell-targeted probe was obtained by immobilizing the AS1411 aptamer over it (denoted as AS1411@PEGMA@GQDs@γ-CD-MOF) and it exhibits pH-responsive release function and excellent targeting ability.
31660562	0	43	theme	metal-organic	23:35	arg1	framework					37:45	A γ-cyclodextrin-based metal-organic framework	0:45	A γ-cyclodextrin-based metal-organic framework	0:45	A γ-cyclodextrin-based metal-organic framework embedded with graphene quantum dots and modified with PEGMA via SI-ATRP for anticancer drug delivery and therapy.
31660562	4	44	theme	sustained	1186:1194	arg1	release					1196:1202	sustained release	1186:1202	sustained release	1186:1202	Large amounts of antitumour drug, doxorubicin hydrochloride (DOX), could be encapsulated within this composite due to the chemical-rich functionality, and the resultant pH-responsive DOX delivery system (denoted as DOX/AS1411@PEGMA@GQDs@γ-CD-MOF) displayed a higher DOX loading of 89.1% with sustained release than the pristine γ-CD-MOF and GQDs@γ-CD-MOF.
31660562	2	45	theme	GQDs	413:416	arg1	γ-CD-MOF					418:425	GQDs@γ-CD-MOF	413:425	GQDs@γ-CD-MOF	413:425	Large amounts of graphene quantum dots (GQDs) were embedded in the γ-CD-MOF matrix (denoted as GQDs@γ-CD-MOF) to endow the γ-CD-MOF with strong fluorescence, which was then modified by pH responsive poly(ethyleneglycol)dimethacrylate (PEGMA) through surface initiated atom transfer radical polymerization (SI-ATRP) to fabricate the PEGMA@GQDs@γ-CD-MOF composite.
31660562	2	46	from	matrix	394:399	arg1	embedded					369:376	embedded	369:376	embedded	369:376	Large amounts of graphene quantum dots (GQDs) were embedded in the γ-CD-MOF matrix (denoted as GQDs@γ-CD-MOF) to endow the γ-CD-MOF with strong fluorescence, which was then modified by pH responsive poly(ethyleneglycol)dimethacrylate (PEGMA) through surface initiated atom transfer radical polymerization (SI-ATRP) to fabricate the PEGMA@GQDs@γ-CD-MOF composite.
31660562	0	47	theme	graphene	61:68	arg1	dots					78:81	graphene quantum dots	61:81	graphene quantum dots	61:81	A γ-cyclodextrin-based metal-organic framework embedded with graphene quantum dots and modified with PEGMA via SI-ATRP for anticancer drug delivery and therapy.
31660562	7	48	theme	tumour	1707:1712	arg1	inhibition					1721:1730	tumour growth inhibition	1707:1730	tumour growth inhibition	1707:1730	Therefore, the proposed AS1411@PEGMA@GQD@γ-CD-MOF composite is promising for effective DOX delivery and tumour growth inhibition both in vitro and in vivo, showing great potential for anticancer therapy.
31660562	2	49	dep	matrix	394:399	arg1	denoted					402:408	denoted	402:408	denoted as GQDs@γ-CD-MOF	402:425	Large amounts of graphene quantum dots (GQDs) were embedded in the γ-CD-MOF matrix (denoted as GQDs@γ-CD-MOF) to endow the γ-CD-MOF with strong fluorescence, which was then modified by pH responsive poly(ethyleneglycol)dimethacrylate (PEGMA) through surface initiated atom transfer radical polymerization (SI-ATRP) to fabricate the PEGMA@GQDs@γ-CD-MOF composite.
31660562	5	50	theme	delivery	1313:1320	arg1	system					1322:1327	this DOX delivery system	1304:1327	this DOX delivery system	1304:1327	The targeting specificity investigation revealed that this DOX delivery system was effectively internalized via receptor mediated endocytosis with high selectivity.
31660562	6	51	dep	in	1419:1420	arg1	vivo					1422:1425	vivo	1422:1425	vivo	1422:1425	The in vivo antitumour study with tumour-bearing mice illustrated that the tumour growth can be effectively suppressed and partially ablated with negligible side effects after treatments.
31660562	4	52	theme	%	1179:1179	arg1	loading					1164:1170	a higher DOX loading	1151:1170	a higher DOX loading of 89.1% with sustained release	1151:1202	Large amounts of antitumour drug, doxorubicin hydrochloride (DOX), could be encapsulated within this composite due to the chemical-rich functionality, and the resultant pH-responsive DOX delivery system (denoted as DOX/AS1411@PEGMA@GQDs@γ-CD-MOF) displayed a higher DOX loading of 89.1% with sustained release than the pristine γ-CD-MOF and GQDs@γ-CD-MOF.
31660562	5	53	theme	specificity	1264:1274	arg1	investigation					1276:1288	The targeting specificity investigation	1250:1288	The targeting specificity investigation	1250:1288	The targeting specificity investigation revealed that this DOX delivery system was effectively internalized via receptor mediated endocytosis with high selectivity.
31660562	4	54	theme	@	1239:1239	arg1	γ-CD-MOF					1240:1247	the pristine γ-CD-MOF and GQDs@γ-CD-MOF	1209:1247	γ-CD-MOF	1240:1247	Large amounts of antitumour drug, doxorubicin hydrochloride (DOX), could be encapsulated within this composite due to the chemical-rich functionality, and the resultant pH-responsive DOX delivery system (denoted as DOX/AS1411@PEGMA@GQDs@γ-CD-MOF) displayed a higher DOX loading of 89.1% with sustained release than the pristine γ-CD-MOF and GQDs@γ-CD-MOF.
31660562	0	55	with	embedded	47:54	arg1	dots					78:81	graphene quantum dots	61:81	graphene quantum dots	61:81	A γ-cyclodextrin-based metal-organic framework embedded with graphene quantum dots and modified with PEGMA via SI-ATRP for anticancer drug delivery and therapy.
31660562	1	56	theme	anticancer	273:282	arg1	delivery					289:296	targeted anticancer drug delivery	264:296	targeted anticancer drug delivery	264:296	The γ-cyclodextrin-based metal-organic framework (γ-CD-MOF) composite was designed and prepared toward targeted anticancer drug delivery and cancer therapy.
31660562	7	57	theme	@	1639:1639	arg1	composite					1653:1661	the proposed AS1411@PEGMA@GQD@γ-CD-MOF composite	1614:1661	the proposed AS1411@PEGMA@GQD@γ-CD-MOF composite	1614:1661	Therefore, the proposed AS1411@PEGMA@GQD@γ-CD-MOF composite is promising for effective DOX delivery and tumour growth inhibition both in vitro and in vivo, showing great potential for anticancer therapy.
31660562	7	57	theme	@	1639:1639	arg1	promising					1666:1674	promising	1666:1674	promising	1666:1674	Therefore, the proposed AS1411@PEGMA@GQD@γ-CD-MOF composite is promising for effective DOX delivery and tumour growth inhibition both in vitro and in vivo, showing great potential for anticancer therapy.
31660562	2	58	theme	initiated	576:584	arg1	SI-ATRP					624:630	SI-ATRP	624:630	SI-ATRP	624:630	Large amounts of graphene quantum dots (GQDs) were embedded in the γ-CD-MOF matrix (denoted as GQDs@γ-CD-MOF) to endow the γ-CD-MOF with strong fluorescence, which was then modified by pH responsive poly(ethyleneglycol)dimethacrylate (PEGMA) through surface initiated atom transfer radical polymerization (SI-ATRP) to fabricate the PEGMA@GQDs@γ-CD-MOF composite.
31660562	2	58	theme	initiated	576:584	arg1	polymerization					608:621	surface initiated atom transfer radical polymerization	568:621	surface initiated atom transfer radical polymerization (SI-ATRP)	568:631	Large amounts of graphene quantum dots (GQDs) were embedded in the γ-CD-MOF matrix (denoted as GQDs@γ-CD-MOF) to endow the γ-CD-MOF with strong fluorescence, which was then modified by pH responsive poly(ethyleneglycol)dimethacrylate (PEGMA) through surface initiated atom transfer radical polymerization (SI-ATRP) to fabricate the PEGMA@GQDs@γ-CD-MOF composite.
31660562	4	59	theme	due	1005:1007	arg1	composite					995:1003	this composite	990:1003	this composite due to the chemical-rich functionality	990:1042	Large amounts of antitumour drug, doxorubicin hydrochloride (DOX), could be encapsulated within this composite due to the chemical-rich functionality, and the resultant pH-responsive DOX delivery system (denoted as DOX/AS1411@PEGMA@GQDs@γ-CD-MOF) displayed a higher DOX loading of 89.1% with sustained release than the pristine γ-CD-MOF and GQDs@γ-CD-MOF.
31660562	5	60	theme	receptor	1362:1369	arg1	endocytosis					1380:1390	receptor mediated endocytosis	1362:1390	receptor mediated endocytosis with high selectivity	1362:1412	The targeting specificity investigation revealed that this DOX delivery system was effectively internalized via receptor mediated endocytosis with high selectivity.
31660562	2	61	theme	Large	318:322	arg1	GQDs					358:361	GQDs	358:361	GQDs	358:361	Large amounts of graphene quantum dots (GQDs) were embedded in the γ-CD-MOF matrix (denoted as GQDs@γ-CD-MOF) to endow the γ-CD-MOF with strong fluorescence, which was then modified by pH responsive poly(ethyleneglycol)dimethacrylate (PEGMA) through surface initiated atom transfer radical polymerization (SI-ATRP) to fabricate the PEGMA@GQDs@γ-CD-MOF composite.
31660562	2	61	theme	Large	318:322	arg1	dots					352:355	graphene quantum dots	335:355	graphene quantum dots (GQDs)	335:362	Large amounts of graphene quantum dots (GQDs) were embedded in the γ-CD-MOF matrix (denoted as GQDs@γ-CD-MOF) to endow the γ-CD-MOF with strong fluorescence, which was then modified by pH responsive poly(ethyleneglycol)dimethacrylate (PEGMA) through surface initiated atom transfer radical polymerization (SI-ATRP) to fabricate the PEGMA@GQDs@γ-CD-MOF composite.
31660562	2	61	theme	Large	318:322	arg1	amounts					324:330	Large amounts	318:330	Large amounts of graphene quantum dots (GQDs)	318:362	Large amounts of graphene quantum dots (GQDs) were embedded in the γ-CD-MOF matrix (denoted as GQDs@γ-CD-MOF) to endow the γ-CD-MOF with strong fluorescence, which was then modified by pH responsive poly(ethyleneglycol)dimethacrylate (PEGMA) through surface initiated atom transfer radical polymerization (SI-ATRP) to fabricate the PEGMA@GQDs@γ-CD-MOF composite.
31660562	4	62	theme	DOX	1077:1079	arg1	system					1090:1095	the resultant pH-responsive DOX delivery system	1049:1095	the resultant pH-responsive DOX delivery system (denoted as DOX/AS1411@PEGMA@GQDs@γ-CD-MOF)	1049:1139	Large amounts of antitumour drug, doxorubicin hydrochloride (DOX), could be encapsulated within this composite due to the chemical-rich functionality, and the resultant pH-responsive DOX delivery system (denoted as DOX/AS1411@PEGMA@GQDs@γ-CD-MOF) displayed a higher DOX loading of 89.1% with sustained release than the pristine γ-CD-MOF and GQDs@γ-CD-MOF.
31660562	4	63	theme	pristine	1213:1220	arg1	γ-CD-MOF					1222:1229	the pristine γ-CD-MOF and GQDs@γ-CD-MOF	1209:1247	γ-CD-MOF	1222:1229	Large amounts of antitumour drug, doxorubicin hydrochloride (DOX), could be encapsulated within this composite due to the chemical-rich functionality, and the resultant pH-responsive DOX delivery system (denoted as DOX/AS1411@PEGMA@GQDs@γ-CD-MOF) displayed a higher DOX loading of 89.1% with sustained release than the pristine γ-CD-MOF and GQDs@γ-CD-MOF.
31660562	1	64	theme	metal-organic	186:198	arg1	composite					221:229	The γ-cyclodextrin-based metal-organic framework (γ-CD-MOF) composite	161:229	The γ-cyclodextrin-based metal-organic framework (γ-CD-MOF) composite	161:229	The γ-cyclodextrin-based metal-organic framework (γ-CD-MOF) composite was designed and prepared toward targeted anticancer drug delivery and cancer therapy.
31660562	2	65	from	embedded	369:376	arg1	matrix					394:399	the γ-CD-MOF matrix	381:399	the γ-CD-MOF matrix (denoted as GQDs@γ-CD-MOF)	381:426	Large amounts of graphene quantum dots (GQDs) were embedded in the γ-CD-MOF matrix (denoted as GQDs@γ-CD-MOF) to endow the γ-CD-MOF with strong fluorescence, which was then modified by pH responsive poly(ethyleneglycol)dimethacrylate (PEGMA) through surface initiated atom transfer radical polymerization (SI-ATRP) to fabricate the PEGMA@GQDs@γ-CD-MOF composite.
31660562	2	66	theme	quantum	344:350	arg1	GQDs					358:361	GQDs	358:361	GQDs	358:361	Large amounts of graphene quantum dots (GQDs) were embedded in the γ-CD-MOF matrix (denoted as GQDs@γ-CD-MOF) to endow the γ-CD-MOF with strong fluorescence, which was then modified by pH responsive poly(ethyleneglycol)dimethacrylate (PEGMA) through surface initiated atom transfer radical polymerization (SI-ATRP) to fabricate the PEGMA@GQDs@γ-CD-MOF composite.
31660562	2	66	theme	quantum	344:350	arg1	dots					352:355	graphene quantum dots	335:355	graphene quantum dots (GQDs)	335:362	Large amounts of graphene quantum dots (GQDs) were embedded in the γ-CD-MOF matrix (denoted as GQDs@γ-CD-MOF) to endow the γ-CD-MOF with strong fluorescence, which was then modified by pH responsive poly(ethyleneglycol)dimethacrylate (PEGMA) through surface initiated atom transfer radical polymerization (SI-ATRP) to fabricate the PEGMA@GQDs@γ-CD-MOF composite.
31660562	2	67	theme	transfer	591:598	arg1	SI-ATRP					624:630	SI-ATRP	624:630	SI-ATRP	624:630	Large amounts of graphene quantum dots (GQDs) were embedded in the γ-CD-MOF matrix (denoted as GQDs@γ-CD-MOF) to endow the γ-CD-MOF with strong fluorescence, which was then modified by pH responsive poly(ethyleneglycol)dimethacrylate (PEGMA) through surface initiated atom transfer radical polymerization (SI-ATRP) to fabricate the PEGMA@GQDs@γ-CD-MOF composite.
31660562	2	67	theme	transfer	591:598	arg1	polymerization					608:621	surface initiated atom transfer radical polymerization	568:621	surface initiated atom transfer radical polymerization (SI-ATRP)	568:631	Large amounts of graphene quantum dots (GQDs) were embedded in the γ-CD-MOF matrix (denoted as GQDs@γ-CD-MOF) to endow the γ-CD-MOF with strong fluorescence, which was then modified by pH responsive poly(ethyleneglycol)dimethacrylate (PEGMA) through surface initiated atom transfer radical polymerization (SI-ATRP) to fabricate the PEGMA@GQDs@γ-CD-MOF composite.
31660562	0	68	theme	drug	134:137	arg1	delivery					139:146	anticancer drug delivery	123:146	anticancer drug delivery	123:146	A γ-cyclodextrin-based metal-organic framework embedded with graphene quantum dots and modified with PEGMA via SI-ATRP for anticancer drug delivery and therapy.
31660562	6	69	theme	tumour-bearing	1449:1462	arg1	mice					1464:1467	tumour-bearing mice	1449:1467	tumour-bearing mice	1449:1467	The in vivo antitumour study with tumour-bearing mice illustrated that the tumour growth can be effectively suppressed and partially ablated with negligible side effects after treatments.
31660562	2	70	theme	ethyleneglycol	522:535	arg1	PEGMA					553:557	PEGMA	553:557	PEGMA	553:557	Large amounts of graphene quantum dots (GQDs) were embedded in the γ-CD-MOF matrix (denoted as GQDs@γ-CD-MOF) to endow the γ-CD-MOF with strong fluorescence, which was then modified by pH responsive poly(ethyleneglycol)dimethacrylate (PEGMA) through surface initiated atom transfer radical polymerization (SI-ATRP) to fabricate the PEGMA@GQDs@γ-CD-MOF composite.
31660562	2	70	theme	ethyleneglycol	522:535	arg1	dimethacrylate					537:550	pH responsive poly(ethyleneglycol)dimethacrylate	503:550	pH responsive poly(ethyleneglycol)dimethacrylate (PEGMA)	503:558	Large amounts of graphene quantum dots (GQDs) were embedded in the γ-CD-MOF matrix (denoted as GQDs@γ-CD-MOF) to endow the γ-CD-MOF with strong fluorescence, which was then modified by pH responsive poly(ethyleneglycol)dimethacrylate (PEGMA) through surface initiated atom transfer radical polymerization (SI-ATRP) to fabricate the PEGMA@GQDs@γ-CD-MOF composite.
31660562	4	71	theme	resultant	1053:1061	arg1	system					1090:1095	the resultant pH-responsive DOX delivery system	1049:1095	the resultant pH-responsive DOX delivery system (denoted as DOX/AS1411@PEGMA@GQDs@γ-CD-MOF)	1049:1139	Large amounts of antitumour drug, doxorubicin hydrochloride (DOX), could be encapsulated within this composite due to the chemical-rich functionality, and the resultant pH-responsive DOX delivery system (denoted as DOX/AS1411@PEGMA@GQDs@γ-CD-MOF) displayed a higher DOX loading of 89.1% with sustained release than the pristine γ-CD-MOF and GQDs@γ-CD-MOF.
31660562	2	72	theme	poly	517:520	arg1	PEGMA					553:557	PEGMA	553:557	PEGMA	553:557	Large amounts of graphene quantum dots (GQDs) were embedded in the γ-CD-MOF matrix (denoted as GQDs@γ-CD-MOF) to endow the γ-CD-MOF with strong fluorescence, which was then modified by pH responsive poly(ethyleneglycol)dimethacrylate (PEGMA) through surface initiated atom transfer radical polymerization (SI-ATRP) to fabricate the PEGMA@GQDs@γ-CD-MOF composite.
31660562	2	72	theme	poly	517:520	arg1	dimethacrylate					537:550	pH responsive poly(ethyleneglycol)dimethacrylate	503:550	pH responsive poly(ethyleneglycol)dimethacrylate (PEGMA)	503:558	Large amounts of graphene quantum dots (GQDs) were embedded in the γ-CD-MOF matrix (denoted as GQDs@γ-CD-MOF) to endow the γ-CD-MOF with strong fluorescence, which was then modified by pH responsive poly(ethyleneglycol)dimethacrylate (PEGMA) through surface initiated atom transfer radical polymerization (SI-ATRP) to fabricate the PEGMA@GQDs@γ-CD-MOF composite.
31660562	4	73	theme	PEGMA	1120:1124	arg1	γ-CD-MOF					1131:1138	DOX/AS1411@PEGMA@GQDs@γ-CD-MOF	1109:1138	DOX/AS1411@PEGMA@GQDs@γ-CD-MOF	1109:1138	Large amounts of antitumour drug, doxorubicin hydrochloride (DOX), could be encapsulated within this composite due to the chemical-rich functionality, and the resultant pH-responsive DOX delivery system (denoted as DOX/AS1411@PEGMA@GQDs@γ-CD-MOF) displayed a higher DOX loading of 89.1% with sustained release than the pristine γ-CD-MOF and GQDs@γ-CD-MOF.
31660562	3	74	theme	AS1411	751:756	arg1	aptamer					758:764	the AS1411 aptamer	747:764	the AS1411 aptamer	747:764	Then, the cancer cell-targeted probe was obtained by immobilizing the AS1411 aptamer over it (denoted as AS1411@PEGMA@GQDs@γ-CD-MOF) and it exhibits pH-responsive release function and excellent targeting ability.
31660562	4	75	theme	GQDs	1126:1129	arg1	γ-CD-MOF					1131:1138	DOX/AS1411@PEGMA@GQDs@γ-CD-MOF	1109:1138	DOX/AS1411@PEGMA@GQDs@γ-CD-MOF	1109:1138	Large amounts of antitumour drug, doxorubicin hydrochloride (DOX), could be encapsulated within this composite due to the chemical-rich functionality, and the resultant pH-responsive DOX delivery system (denoted as DOX/AS1411@PEGMA@GQDs@γ-CD-MOF) displayed a higher DOX loading of 89.1% with sustained release than the pristine γ-CD-MOF and GQDs@γ-CD-MOF.
31660562	3	76	theme	@	803:803	arg1	γ-CD-MOF					804:811	AS1411@PEGMA@GQDs@γ-CD-MOF	786:811	AS1411@PEGMA@GQDs@γ-CD-MOF	786:811	Then, the cancer cell-targeted probe was obtained by immobilizing the AS1411 aptamer over it (denoted as AS1411@PEGMA@GQDs@γ-CD-MOF) and it exhibits pH-responsive release function and excellent targeting ability.
31660562	2	77	with	γ-CD-MOF	441:448	arg1	fluorescence					462:473	strong fluorescence	455:473	strong fluorescence	455:473	Large amounts of graphene quantum dots (GQDs) were embedded in the γ-CD-MOF matrix (denoted as GQDs@γ-CD-MOF) to endow the γ-CD-MOF with strong fluorescence, which was then modified by pH responsive poly(ethyleneglycol)dimethacrylate (PEGMA) through surface initiated atom transfer radical polymerization (SI-ATRP) to fabricate the PEGMA@GQDs@γ-CD-MOF composite.
31660562	3	78	theme	@	798:798	arg1	γ-CD-MOF					804:811	AS1411@PEGMA@GQDs@γ-CD-MOF	786:811	AS1411@PEGMA@GQDs@γ-CD-MOF	786:811	Then, the cancer cell-targeted probe was obtained by immobilizing the AS1411 aptamer over it (denoted as AS1411@PEGMA@GQDs@γ-CD-MOF) and it exhibits pH-responsive release function and excellent targeting ability.
31660562	4	79	theme	antitumour	911:920	arg1	drug					922:925	antitumour drug	911:925	antitumour drug	911:925	Large amounts of antitumour drug, doxorubicin hydrochloride (DOX), could be encapsulated within this composite due to the chemical-rich functionality, and the resultant pH-responsive DOX delivery system (denoted as DOX/AS1411@PEGMA@GQDs@γ-CD-MOF) displayed a higher DOX loading of 89.1% with sustained release than the pristine γ-CD-MOF and GQDs@γ-CD-MOF.
31660562	4	79	theme	antitumour	911:920	arg1	hydrochloride					940:952	doxorubicin hydrochloride	928:952	doxorubicin hydrochloride (DOX)	928:958	Large amounts of antitumour drug, doxorubicin hydrochloride (DOX), could be encapsulated within this composite due to the chemical-rich functionality, and the resultant pH-responsive DOX delivery system (denoted as DOX/AS1411@PEGMA@GQDs@γ-CD-MOF) displayed a higher DOX loading of 89.1% with sustained release than the pristine γ-CD-MOF and GQDs@γ-CD-MOF.
31660562	4	80	theme	DOX/AS1411	1109:1118	arg1	γ-CD-MOF					1131:1138	DOX/AS1411@PEGMA@GQDs@γ-CD-MOF	1109:1138	DOX/AS1411@PEGMA@GQDs@γ-CD-MOF	1109:1138	Large amounts of antitumour drug, doxorubicin hydrochloride (DOX), could be encapsulated within this composite due to the chemical-rich functionality, and the resultant pH-responsive DOX delivery system (denoted as DOX/AS1411@PEGMA@GQDs@γ-CD-MOF) displayed a higher DOX loading of 89.1% with sustained release than the pristine γ-CD-MOF and GQDs@γ-CD-MOF.
31660562	2	81	mod	modified	491:498	arg3	dimethacrylate					537:550	pH responsive poly(ethyleneglycol)dimethacrylate	503:550	pH responsive poly(ethyleneglycol)dimethacrylate (PEGMA)	503:558	Large amounts of graphene quantum dots (GQDs) were embedded in the γ-CD-MOF matrix (denoted as GQDs@γ-CD-MOF) to endow the γ-CD-MOF with strong fluorescence, which was then modified by pH responsive poly(ethyleneglycol)dimethacrylate (PEGMA) through surface initiated atom transfer radical polymerization (SI-ATRP) to fabricate the PEGMA@GQDs@γ-CD-MOF composite.
31660562	2	81	mod	modified	491:498	arg3	PEGMA					553:557	PEGMA	553:557	PEGMA	553:557	Large amounts of graphene quantum dots (GQDs) were embedded in the γ-CD-MOF matrix (denoted as GQDs@γ-CD-MOF) to endow the γ-CD-MOF with strong fluorescence, which was then modified by pH responsive poly(ethyleneglycol)dimethacrylate (PEGMA) through surface initiated atom transfer radical polymerization (SI-ATRP) to fabricate the PEGMA@GQDs@γ-CD-MOF composite.
31660562	2	81	mod	modified	491:498	arg1	fluorescence					462:473	strong fluorescence	455:473	strong fluorescence	455:473	Large amounts of graphene quantum dots (GQDs) were embedded in the γ-CD-MOF matrix (denoted as GQDs@γ-CD-MOF) to endow the γ-CD-MOF with strong fluorescence, which was then modified by pH responsive poly(ethyleneglycol)dimethacrylate (PEGMA) through surface initiated atom transfer radical polymerization (SI-ATRP) to fabricate the PEGMA@GQDs@γ-CD-MOF composite.
31660562	4	82	theme	higher	1153:1158	arg1	loading					1164:1170	a higher DOX loading	1151:1170	a higher DOX loading of 89.1% with sustained release	1151:1202	Large amounts of antitumour drug, doxorubicin hydrochloride (DOX), could be encapsulated within this composite due to the chemical-rich functionality, and the resultant pH-responsive DOX delivery system (denoted as DOX/AS1411@PEGMA@GQDs@γ-CD-MOF) displayed a higher DOX loading of 89.1% with sustained release than the pristine γ-CD-MOF and GQDs@γ-CD-MOF.
31660562	3	83	theme	@	792:792	arg1	γ-CD-MOF					804:811	AS1411@PEGMA@GQDs@γ-CD-MOF	786:811	AS1411@PEGMA@GQDs@γ-CD-MOF	786:811	Then, the cancer cell-targeted probe was obtained by immobilizing the AS1411 aptamer over it (denoted as AS1411@PEGMA@GQDs@γ-CD-MOF) and it exhibits pH-responsive release function and excellent targeting ability.
31660562	7	84	theme	γ-CD-MOF	1644:1651	arg1	composite					1653:1661	the proposed AS1411@PEGMA@GQD@γ-CD-MOF composite	1614:1661	the proposed AS1411@PEGMA@GQD@γ-CD-MOF composite	1614:1661	Therefore, the proposed AS1411@PEGMA@GQD@γ-CD-MOF composite is promising for effective DOX delivery and tumour growth inhibition both in vitro and in vivo, showing great potential for anticancer therapy.
31660562	7	84	theme	γ-CD-MOF	1644:1651	arg1	promising					1666:1674	promising	1666:1674	promising	1666:1674	Therefore, the proposed AS1411@PEGMA@GQD@γ-CD-MOF composite is promising for effective DOX delivery and tumour growth inhibition both in vitro and in vivo, showing great potential for anticancer therapy.
31660562	2	85	theme	pH	503:504	arg1	PEGMA					553:557	PEGMA	553:557	PEGMA	553:557	Large amounts of graphene quantum dots (GQDs) were embedded in the γ-CD-MOF matrix (denoted as GQDs@γ-CD-MOF) to endow the γ-CD-MOF with strong fluorescence, which was then modified by pH responsive poly(ethyleneglycol)dimethacrylate (PEGMA) through surface initiated atom transfer radical polymerization (SI-ATRP) to fabricate the PEGMA@GQDs@γ-CD-MOF composite.
31660562	2	85	theme	pH	503:504	arg1	dimethacrylate					537:550	pH responsive poly(ethyleneglycol)dimethacrylate	503:550	pH responsive poly(ethyleneglycol)dimethacrylate (PEGMA)	503:558	Large amounts of graphene quantum dots (GQDs) were embedded in the γ-CD-MOF matrix (denoted as GQDs@γ-CD-MOF) to endow the γ-CD-MOF with strong fluorescence, which was then modified by pH responsive poly(ethyleneglycol)dimethacrylate (PEGMA) through surface initiated atom transfer radical polymerization (SI-ATRP) to fabricate the PEGMA@GQDs@γ-CD-MOF composite.
31660562	3	86	dep	immobilizing	734:745	arg1	denoted					775:781	denoted	775:781	denoted as AS1411@PEGMA@GQDs@γ-CD-MOF	775:811	Then, the cancer cell-targeted probe was obtained by immobilizing the AS1411 aptamer over it (denoted as AS1411@PEGMA@GQDs@γ-CD-MOF) and it exhibits pH-responsive release function and excellent targeting ability.
31660562	7	87	theme	GQD	1640:1642	arg1	composite					1653:1661	the proposed AS1411@PEGMA@GQD@γ-CD-MOF composite	1614:1661	the proposed AS1411@PEGMA@GQD@γ-CD-MOF composite	1614:1661	Therefore, the proposed AS1411@PEGMA@GQD@γ-CD-MOF composite is promising for effective DOX delivery and tumour growth inhibition both in vitro and in vivo, showing great potential for anticancer therapy.
31660562	7	87	theme	GQD	1640:1642	arg1	promising					1666:1674	promising	1666:1674	promising	1666:1674	Therefore, the proposed AS1411@PEGMA@GQD@γ-CD-MOF composite is promising for effective DOX delivery and tumour growth inhibition both in vitro and in vivo, showing great potential for anticancer therapy.
31660562	5	88	theme	high	1397:1400	arg1	selectivity					1402:1412	high selectivity	1397:1412	high selectivity	1397:1412	The targeting specificity investigation revealed that this DOX delivery system was effectively internalized via receptor mediated endocytosis with high selectivity.
31660562	2	89	theme	GQDs	656:659	arg1	composite					670:678	the PEGMA@GQDs@γ-CD-MOF composite	646:678	the PEGMA@GQDs@γ-CD-MOF composite	646:678	Large amounts of graphene quantum dots (GQDs) were embedded in the γ-CD-MOF matrix (denoted as GQDs@γ-CD-MOF) to endow the γ-CD-MOF with strong fluorescence, which was then modified by pH responsive poly(ethyleneglycol)dimethacrylate (PEGMA) through surface initiated atom transfer radical polymerization (SI-ATRP) to fabricate the PEGMA@GQDs@γ-CD-MOF composite.
31660562	0	90	theme	γ-cyclodextrin-based	2:21	arg1	framework					37:45	A γ-cyclodextrin-based metal-organic framework	0:45	A γ-cyclodextrin-based metal-organic framework	0:45	A γ-cyclodextrin-based metal-organic framework embedded with graphene quantum dots and modified with PEGMA via SI-ATRP for anticancer drug delivery and therapy.
31660562	6	91	theme	side	1572:1575	arg1	effects					1577:1583	negligible side effects	1561:1583	negligible side effects after treatments	1561:1600	The in vivo antitumour study with tumour-bearing mice illustrated that the tumour growth can be effectively suppressed and partially ablated with negligible side effects after treatments.
31660562	4	92	theme	Large	894:898	arg1	amounts					900:906	Large amounts	894:906	Large amounts of antitumour drug, doxorubicin hydrochloride (DOX),	894:959	Large amounts of antitumour drug, doxorubicin hydrochloride (DOX), could be encapsulated within this composite due to the chemical-rich functionality, and the resultant pH-responsive DOX delivery system (denoted as DOX/AS1411@PEGMA@GQDs@γ-CD-MOF) displayed a higher DOX loading of 89.1% with sustained release than the pristine γ-CD-MOF and GQDs@γ-CD-MOF.
31660562	4	92	theme	Large	894:898	arg1	drug					922:925	antitumour drug	911:925	antitumour drug	911:925	Large amounts of antitumour drug, doxorubicin hydrochloride (DOX), could be encapsulated within this composite due to the chemical-rich functionality, and the resultant pH-responsive DOX delivery system (denoted as DOX/AS1411@PEGMA@GQDs@γ-CD-MOF) displayed a higher DOX loading of 89.1% with sustained release than the pristine γ-CD-MOF and GQDs@γ-CD-MOF.
31660562	4	92	theme	Large	894:898	arg1	hydrochloride					940:952	doxorubicin hydrochloride	928:952	doxorubicin hydrochloride (DOX)	928:958	Large amounts of antitumour drug, doxorubicin hydrochloride (DOX), could be encapsulated within this composite due to the chemical-rich functionality, and the resultant pH-responsive DOX delivery system (denoted as DOX/AS1411@PEGMA@GQDs@γ-CD-MOF) displayed a higher DOX loading of 89.1% with sustained release than the pristine γ-CD-MOF and GQDs@γ-CD-MOF.
31660562	3	93	theme	pH-responsive	830:842	arg1	function					852:859	pH-responsive release function	830:859	pH-responsive release function	830:859	Then, the cancer cell-targeted probe was obtained by immobilizing the AS1411 aptamer over it (denoted as AS1411@PEGMA@GQDs@γ-CD-MOF) and it exhibits pH-responsive release function and excellent targeting ability.
31660562	2	94	theme	PEGMA	650:654	arg1	composite					670:678	the PEGMA@GQDs@γ-CD-MOF composite	646:678	the PEGMA@GQDs@γ-CD-MOF composite	646:678	Large amounts of graphene quantum dots (GQDs) were embedded in the γ-CD-MOF matrix (denoted as GQDs@γ-CD-MOF) to endow the γ-CD-MOF with strong fluorescence, which was then modified by pH responsive poly(ethyleneglycol)dimethacrylate (PEGMA) through surface initiated atom transfer radical polymerization (SI-ATRP) to fabricate the PEGMA@GQDs@γ-CD-MOF composite.
31660562	2	95	theme	γ-CD-MOF	385:392	arg1	matrix					394:399	the γ-CD-MOF matrix	381:399	the γ-CD-MOF matrix (denoted as GQDs@γ-CD-MOF)	381:426	Large amounts of graphene quantum dots (GQDs) were embedded in the γ-CD-MOF matrix (denoted as GQDs@γ-CD-MOF) to endow the γ-CD-MOF with strong fluorescence, which was then modified by pH responsive poly(ethyleneglycol)dimethacrylate (PEGMA) through surface initiated atom transfer radical polymerization (SI-ATRP) to fabricate the PEGMA@GQDs@γ-CD-MOF composite.
31660562	7	96	theme	AS1411	1627:1632	arg1	composite					1653:1661	the proposed AS1411@PEGMA@GQD@γ-CD-MOF composite	1614:1661	the proposed AS1411@PEGMA@GQD@γ-CD-MOF composite	1614:1661	Therefore, the proposed AS1411@PEGMA@GQD@γ-CD-MOF composite is promising for effective DOX delivery and tumour growth inhibition both in vitro and in vivo, showing great potential for anticancer therapy.
31660562	7	96	theme	AS1411	1627:1632	arg1	promising					1666:1674	promising	1666:1674	promising	1666:1674	Therefore, the proposed AS1411@PEGMA@GQD@γ-CD-MOF composite is promising for effective DOX delivery and tumour growth inhibition both in vitro and in vivo, showing great potential for anticancer therapy.
31660562	2	97	theme	@	417:417	arg1	γ-CD-MOF					418:425	GQDs@γ-CD-MOF	413:425	GQDs@γ-CD-MOF	413:425	Large amounts of graphene quantum dots (GQDs) were embedded in the γ-CD-MOF matrix (denoted as GQDs@γ-CD-MOF) to endow the γ-CD-MOF with strong fluorescence, which was then modified by pH responsive poly(ethyleneglycol)dimethacrylate (PEGMA) through surface initiated atom transfer radical polymerization (SI-ATRP) to fabricate the PEGMA@GQDs@γ-CD-MOF composite.
31660562	3	98	theme	excellent	865:873	arg1	ability					885:891	excellent targeting ability	865:891	excellent targeting ability	865:891	Then, the cancer cell-targeted probe was obtained by immobilizing the AS1411 aptamer over it (denoted as AS1411@PEGMA@GQDs@γ-CD-MOF) and it exhibits pH-responsive release function and excellent targeting ability.
31660562	2	99	theme	strong	455:460	arg1	fluorescence					462:473	strong fluorescence	455:473	strong fluorescence	455:473	Large amounts of graphene quantum dots (GQDs) were embedded in the γ-CD-MOF matrix (denoted as GQDs@γ-CD-MOF) to endow the γ-CD-MOF with strong fluorescence, which was then modified by pH responsive poly(ethyleneglycol)dimethacrylate (PEGMA) through surface initiated atom transfer radical polymerization (SI-ATRP) to fabricate the PEGMA@GQDs@γ-CD-MOF composite.
31660562	0	100	theme	quantum	70:76	arg1	dots					78:81	graphene quantum dots	61:81	graphene quantum dots	61:81	A γ-cyclodextrin-based metal-organic framework embedded with graphene quantum dots and modified with PEGMA via SI-ATRP for anticancer drug delivery and therapy.
31660562	5	101	with	endocytosis	1380:1390	arg1	selectivity					1402:1412	high selectivity	1397:1412	high selectivity	1397:1412	The targeting specificity investigation revealed that this DOX delivery system was effectively internalized via receptor mediated endocytosis with high selectivity.
31660562	5	102	theme	DOX	1309:1311	arg1	system					1322:1327	this DOX delivery system	1304:1327	this DOX delivery system	1304:1327	The targeting specificity investigation revealed that this DOX delivery system was effectively internalized via receptor mediated endocytosis with high selectivity.
31660562	6	103	theme	antitumour	1427:1436	arg1	study					1438:1442	The in vivo antitumour study	1415:1442	The in vivo antitumour study with tumour-bearing mice	1415:1467	The in vivo antitumour study with tumour-bearing mice illustrated that the tumour growth can be effectively suppressed and partially ablated with negligible side effects after treatments.
31660562	7	104	theme	anticancer	1787:1796	arg1	therapy					1798:1804	anticancer therapy	1787:1804	anticancer therapy	1787:1804	Therefore, the proposed AS1411@PEGMA@GQD@γ-CD-MOF composite is promising for effective DOX delivery and tumour growth inhibition both in vitro and in vivo, showing great potential for anticancer therapy.
31660562	7	105	theme	great	1767:1771	arg1	potential					1773:1781	great potential	1767:1781	great potential for anticancer therapy	1767:1804	Therefore, the proposed AS1411@PEGMA@GQD@γ-CD-MOF composite is promising for effective DOX delivery and tumour growth inhibition both in vitro and in vivo, showing great potential for anticancer therapy.
31660562	5	106	theme	targeting	1254:1262	arg1	investigation					1276:1288	The targeting specificity investigation	1250:1288	The targeting specificity investigation	1250:1288	The targeting specificity investigation revealed that this DOX delivery system was effectively internalized via receptor mediated endocytosis with high selectivity.
31660562	2	107	theme	dots	352:355	arg1	GQDs					358:361	GQDs	358:361	GQDs	358:361	Large amounts of graphene quantum dots (GQDs) were embedded in the γ-CD-MOF matrix (denoted as GQDs@γ-CD-MOF) to endow the γ-CD-MOF with strong fluorescence, which was then modified by pH responsive poly(ethyleneglycol)dimethacrylate (PEGMA) through surface initiated atom transfer radical polymerization (SI-ATRP) to fabricate the PEGMA@GQDs@γ-CD-MOF composite.
31660562	2	107	theme	dots	352:355	arg1	dots					352:355	graphene quantum dots	335:355	graphene quantum dots (GQDs)	335:362	Large amounts of graphene quantum dots (GQDs) were embedded in the γ-CD-MOF matrix (denoted as GQDs@γ-CD-MOF) to endow the γ-CD-MOF with strong fluorescence, which was then modified by pH responsive poly(ethyleneglycol)dimethacrylate (PEGMA) through surface initiated atom transfer radical polymerization (SI-ATRP) to fabricate the PEGMA@GQDs@γ-CD-MOF composite.
31660562	2	107	theme	dots	352:355	arg1	amounts					324:330	Large amounts	318:330	Large amounts of graphene quantum dots (GQDs)	318:362	Large amounts of graphene quantum dots (GQDs) were embedded in the γ-CD-MOF matrix (denoted as GQDs@γ-CD-MOF) to endow the γ-CD-MOF with strong fluorescence, which was then modified by pH responsive poly(ethyleneglycol)dimethacrylate (PEGMA) through surface initiated atom transfer radical polymerization (SI-ATRP) to fabricate the PEGMA@GQDs@γ-CD-MOF composite.
31660562	6	108	theme	in	1419:1420	arg1	study					1438:1442	The in vivo antitumour study	1415:1442	The in vivo antitumour study with tumour-bearing mice	1415:1467	The in vivo antitumour study with tumour-bearing mice illustrated that the tumour growth can be effectively suppressed and partially ablated with negligible side effects after treatments.
31660562	4	109	theme	chemical-rich	1016:1028	arg1	functionality					1030:1042	the chemical-rich functionality	1012:1042	the chemical-rich functionality	1012:1042	Large amounts of antitumour drug, doxorubicin hydrochloride (DOX), could be encapsulated within this composite due to the chemical-rich functionality, and the resultant pH-responsive DOX delivery system (denoted as DOX/AS1411@PEGMA@GQDs@γ-CD-MOF) displayed a higher DOX loading of 89.1% with sustained release than the pristine γ-CD-MOF and GQDs@γ-CD-MOF.
31660562	1	110	theme	targeted	264:271	arg1	delivery					289:296	targeted anticancer drug delivery	264:296	targeted anticancer drug delivery	264:296	The γ-cyclodextrin-based metal-organic framework (γ-CD-MOF) composite was designed and prepared toward targeted anticancer drug delivery and cancer therapy.
31389129	6	0	theme	cholesterol	825:835	arg1	levels					855:860	plasma total cholesterol and triglycerides levels	812:860	plasma total cholesterol and triglycerides levels	812:860	A. muciniphila administration lowers body weight and plasma total cholesterol and triglycerides levels.
31389129	7	1	theme	immune	893:898	arg1	composition					905:915	the immune cell composition	889:915	the immune cell composition in mesenteric lymph nodes	889:941	A. muciniphila influences the immune cell composition in mesenteric lymph nodes, as evident from an increased total B cell population, while reducing the total T cell and neutrophil populations.
31389129	11	2	theme	treatment	1445:1453	arg1	weeks					1436:1440	CONCLUSIONS Four weeks	1419:1440	CONCLUSIONS Four weeks of treatment with A. muciniphila	1419:1473	CONCLUSIONS Four weeks of treatment with A. muciniphila exerts lipid-lowering and immunomodulatory effects, which are insufficient to inhibit neointima formation in hyperlipidemic E3L.CETP mice.
31389129	6	3	theme	plasma	812:817	arg1	cholesterol					825:835	plasma total cholesterol	812:835	plasma total cholesterol	812:835	A. muciniphila administration lowers body weight and plasma total cholesterol and triglycerides levels.
31389129	2	4	dep	intestine	276:284	arg1	the					272:274	the	272:274	the	272:274	SCOPE Akkermansia muciniphila (A. muciniphila) is an intestinal commensal with anti-inflammatory properties both in the intestine and other organs.
31389129	2	5	theme	Akkermansia	162:172	arg1	commensal					220:228	an intestinal commensal	206:228	an intestinal commensal with anti-inflammatory properties both in the intestine and other organs	206:301	SCOPE Akkermansia muciniphila (A. muciniphila) is an intestinal commensal with anti-inflammatory properties both in the intestine and other organs.
31389129	2	5	theme	Akkermansia	162:172	arg1	muciniphila					174:184	SCOPE Akkermansia muciniphila	156:184	SCOPE Akkermansia muciniphila (A. muciniphila)	156:201	SCOPE Akkermansia muciniphila (A. muciniphila) is an intestinal commensal with anti-inflammatory properties both in the intestine and other organs.
31389129	8	6	dep	markers	1127:1133	arg1	markers					1127:1133	the activation markers MHCII on dendritic cells and CD86	1112:1167	the activation markers MHCII on dendritic cells and CD86	1112:1167	Importantly, A. muciniphila reduces the expression of the activation markers MHCII on dendritic cells and CD86 on B cells.
31389129	8	6	dep	markers	1127:1133	arg1	CD86					1164:1167	CD86	1164:1167	CD86	1164:1167	Importantly, A. muciniphila reduces the expression of the activation markers MHCII on dendritic cells and CD86 on B cells.
31389129	8	6	dep	markers	1127:1133	arg1	MHCII					1135:1139	MHCII	1135:1139	MHCII on dendritic cells	1135:1158	Importantly, A. muciniphila reduces the expression of the activation markers MHCII on dendritic cells and CD86 on B cells.
31389129	11	7	theme	E3L.CETP	1599:1606	arg1	mice					1608:1611	hyperlipidemic E3L.CETP mice	1584:1611	hyperlipidemic E3L.CETP mice	1584:1611	CONCLUSIONS Four weeks of treatment with A. muciniphila exerts lipid-lowering and immunomodulatory effects, which are insufficient to inhibit neointima formation in hyperlipidemic E3L.CETP mice.
31389129	2	8	dep	properties	253:262	arg1	both					264:267	both	264:267	both	264:267	SCOPE Akkermansia muciniphila (A. muciniphila) is an intestinal commensal with anti-inflammatory properties both in the intestine and other organs.
31389129	0	9	from	Formation	103:111	arg1	3-Leiden					136:143	Hyperlipidemic APOE*3-Leiden	116:143	Hyperlipidemic APOE*3-Leiden	116:143	Akkermansia muciniphila Exerts Lipid-Lowering and Immunomodulatory Effects without Affecting Neointima Formation in Hyperlipidemic APOE*3-Leiden.
31389129	9	10	theme	IL-10	1261:1265	arg1	release					1267:1273	ex vivo lipopolysaccharide-stimulated IL-10 release	1223:1273	ex vivo lipopolysaccharide-stimulated IL-10 release	1223:1273	A. muciniphila also increases whole blood ex vivo lipopolysaccharide-stimulated IL-10 release.
31389129	7	11	theme	T	1023:1023	arg1	cell					1025:1028	the total T cell and neutrophil populations	1013:1055	cell	1025:1028	A. muciniphila influences the immune cell composition in mesenteric lymph nodes, as evident from an increased total B cell population, while reducing the total T cell and neutrophil populations.
31389129	5	12	theme	lipid	672:676	arg1	levels					678:683	plasma lipid levels	665:683	plasma lipid levels	665:683	METHODS AND RESULTS Hyperlipidemic male E3L.CETP mice are daily treated with 2 × 108 CFU A. muciniphila by oral gavage for 4 weeks and the effects are determined on plasma lipid levels, immune parameters, and cuff-induced neointima formation and composition.
31389129	3	13	from	effects	334:340	arg1	formation					441:449	cuff-induced neointima formation	418:449	cuff-induced neointima formation in hyperlipidemic APOE*3-Leiden (E3L)	418:487	The aim is to investigate the effects of oral administration of A. muciniphila on lipid metabolism, immunity, and cuff-induced neointima formation in hyperlipidemic APOE*3-Leiden (E3L).
31389129	3	13	from	effects	334:340	arg1	immunity					404:411	immunity	404:411	immunity	404:411	The aim is to investigate the effects of oral administration of A. muciniphila on lipid metabolism, immunity, and cuff-induced neointima formation in hyperlipidemic APOE*3-Leiden (E3L).
31389129	3	13	from	effects	334:340	arg1	metabolism					392:401	lipid metabolism	386:401	lipid metabolism	386:401	The aim is to investigate the effects of oral administration of A. muciniphila on lipid metabolism, immunity, and cuff-induced neointima formation in hyperlipidemic APOE*3-Leiden (E3L).
31389129	2	14	theme	anti-inflammatory	235:251	arg1	properties					253:262	anti-inflammatory properties	235:262	anti-inflammatory properties	235:262	SCOPE Akkermansia muciniphila (A. muciniphila) is an intestinal commensal with anti-inflammatory properties both in the intestine and other organs.
31389129	1	15	theme	CETP	145:148	arg1	Mice					150:153	CETP Mice	145:153	CETP Mice.	145:154	CETP Mice.
31389129	5	16	dep	METHODS	500:506	arg1	determined					651:660	determined	651:660	are determined on plasma lipid levels, immune parameters, and cuff-induced neointima formation and composition	647:756	METHODS AND RESULTS Hyperlipidemic male E3L.CETP mice are daily treated with 2 × 108 CFU A. muciniphila by oral gavage for 4 weeks and the effects are determined on plasma lipid levels, immune parameters, and cuff-induced neointima formation and composition.
31389129	5	16	dep	METHODS	500:506	arg1	treated					564:570	treated	564:570	are daily treated with 2 × 108 CFU A. muciniphila by oral gavage for 4 weeks	554:629	METHODS AND RESULTS Hyperlipidemic male E3L.CETP mice are daily treated with 2 × 108 CFU A. muciniphila by oral gavage for 4 weeks and the effects are determined on plasma lipid levels, immune parameters, and cuff-induced neointima formation and composition.
31389129	7	17	theme	neutrophil	1034:1043	arg1	populations					1045:1055	the total T cell and neutrophil populations	1013:1055	populations	1045:1055	A. muciniphila influences the immune cell composition in mesenteric lymph nodes, as evident from an increased total B cell population, while reducing the total T cell and neutrophil populations.
31389129	5	18	theme	Hyperlipidemic	520:533	arg1	mice					549:552	Hyperlipidemic male E3L.CETP mice	520:552	Hyperlipidemic male E3L.CETP mice	520:552	METHODS AND RESULTS Hyperlipidemic male E3L.CETP mice are daily treated with 2 × 108 CFU A. muciniphila by oral gavage for 4 weeks and the effects are determined on plasma lipid levels, immune parameters, and cuff-induced neointima formation and composition.
31389129	8	19	from	expression	1098:1107	arg1	cells					1174:1178	B cells	1172:1178	B cells	1172:1178	Importantly, A. muciniphila reduces the expression of the activation markers MHCII on dendritic cells and CD86 on B cells.
31389129	3	20	theme	oral	345:348	arg1	administration					350:363	oral administration	345:363	oral administration of A. muciniphila	345:381	The aim is to investigate the effects of oral administration of A. muciniphila on lipid metabolism, immunity, and cuff-induced neointima formation in hyperlipidemic APOE*3-Leiden (E3L).
31389129	7	21	theme	total	1017:1021	arg1	cell					1025:1028	the total T cell and neutrophil populations	1013:1055	cell	1025:1028	A. muciniphila influences the immune cell composition in mesenteric lymph nodes, as evident from an increased total B cell population, while reducing the total T cell and neutrophil populations.
31389129	5	22	theme	E3L.CETP	540:547	arg1	mice					549:552	Hyperlipidemic male E3L.CETP mice	520:552	Hyperlipidemic male E3L.CETP mice	520:552	METHODS AND RESULTS Hyperlipidemic male E3L.CETP mice are daily treated with 2 × 108 CFU A. muciniphila by oral gavage for 4 weeks and the effects are determined on plasma lipid levels, immune parameters, and cuff-induced neointima formation and composition.
31389129	10	23	with	treatment	1294:1302	arg1	muciniphila					1312:1322	A. muciniphila	1309:1322	A. muciniphila	1309:1322	Finally, although treatment with A. muciniphila improves lipid metabolism and immunity, it does not affect neointima formation or composition.
31389129	7	24	theme	B	979:979	arg1	population					986:995	an increased total B cell population	960:995	an increased total B cell population	960:995	A. muciniphila influences the immune cell composition in mesenteric lymph nodes, as evident from an increased total B cell population, while reducing the total T cell and neutrophil populations.
31389129	3	25	theme	muciniphila	371:381	arg1	administration					350:363	oral administration	345:363	oral administration of A. muciniphila	345:381	The aim is to investigate the effects of oral administration of A. muciniphila on lipid metabolism, immunity, and cuff-induced neointima formation in hyperlipidemic APOE*3-Leiden (E3L).
31389129	0	26	theme	Hyperlipidemic	116:129	arg1	3-Leiden					136:143	Hyperlipidemic APOE*3-Leiden	116:143	Hyperlipidemic APOE*3-Leiden	116:143	Akkermansia muciniphila Exerts Lipid-Lowering and Immunomodulatory Effects without Affecting Neointima Formation in Hyperlipidemic APOE*3-Leiden.
31389129	7	27	theme	increased	963:971	arg1	population					986:995	an increased total B cell population	960:995	an increased total B cell population	960:995	A. muciniphila influences the immune cell composition in mesenteric lymph nodes, as evident from an increased total B cell population, while reducing the total T cell and neutrophil populations.
31389129	10	28	theme	lipid	1333:1337	arg1	metabolism					1339:1348	lipid metabolism	1333:1348	lipid metabolism	1333:1348	Finally, although treatment with A. muciniphila improves lipid metabolism and immunity, it does not affect neointima formation or composition.
31389129	7	29	from	composition	905:915	arg1	nodes					937:941	mesenteric lymph nodes	920:941	mesenteric lymph nodes	920:941	A. muciniphila influences the immune cell composition in mesenteric lymph nodes, as evident from an increased total B cell population, while reducing the total T cell and neutrophil populations.
31389129	3	30	theme	hyperlipidemic	454:467	arg1	3-Leiden					474:481	hyperlipidemic APOE*3-Leiden	454:481	hyperlipidemic APOE*3-Leiden (E3L)	454:487	The aim is to investigate the effects of oral administration of A. muciniphila on lipid metabolism, immunity, and cuff-induced neointima formation in hyperlipidemic APOE*3-Leiden (E3L).
31389129	3	30	theme	hyperlipidemic	454:467	arg1	E3L					484:486	E3L	484:486	E3L	484:486	The aim is to investigate the effects of oral administration of A. muciniphila on lipid metabolism, immunity, and cuff-induced neointima formation in hyperlipidemic APOE*3-Leiden (E3L).
31389129	0	31	theme	*	135:135	arg1	3-Leiden					136:143	Hyperlipidemic APOE*3-Leiden	116:143	Hyperlipidemic APOE*3-Leiden	116:143	Akkermansia muciniphila Exerts Lipid-Lowering and Immunomodulatory Effects without Affecting Neointima Formation in Hyperlipidemic APOE*3-Leiden.
31389129	4	32	theme	CETP	489:492	arg1	mice					494:497	CETP mice	489:497	CETP mice.	489:498	CETP mice.
31389129	0	33	theme	Akkermansia	0:10	arg1	muciniphila					12:22	Akkermansia muciniphila	0:22	Akkermansia muciniphila	0:22	Akkermansia muciniphila Exerts Lipid-Lowering and Immunomodulatory Effects without Affecting Neointima Formation in Hyperlipidemic APOE*3-Leiden.
31389129	3	34	theme	lipid	386:390	arg1	metabolism					392:401	lipid metabolism	386:401	lipid metabolism	386:401	The aim is to investigate the effects of oral administration of A. muciniphila on lipid metabolism, immunity, and cuff-induced neointima formation in hyperlipidemic APOE*3-Leiden (E3L).
31389129	3	35	theme	cuff-induced	418:429	arg1	formation					441:449	cuff-induced neointima formation	418:449	cuff-induced neointima formation in hyperlipidemic APOE*3-Leiden (E3L)	418:487	The aim is to investigate the effects of oral administration of A. muciniphila on lipid metabolism, immunity, and cuff-induced neointima formation in hyperlipidemic APOE*3-Leiden (E3L).
31389129	2	36	dep	muciniphila	174:184	arg1	muciniphila					190:200	A. muciniphila	187:200	A. muciniphila	187:200	SCOPE Akkermansia muciniphila (A. muciniphila) is an intestinal commensal with anti-inflammatory properties both in the intestine and other organs.
31389129	3	37	theme	*	473:473	arg1	3-Leiden					474:481	hyperlipidemic APOE*3-Leiden	454:481	hyperlipidemic APOE*3-Leiden (E3L)	454:487	The aim is to investigate the effects of oral administration of A. muciniphila on lipid metabolism, immunity, and cuff-induced neointima formation in hyperlipidemic APOE*3-Leiden (E3L).
31389129	3	37	theme	*	473:473	arg1	E3L					484:486	E3L	484:486	E3L	484:486	The aim is to investigate the effects of oral administration of A. muciniphila on lipid metabolism, immunity, and cuff-induced neointima formation in hyperlipidemic APOE*3-Leiden (E3L).
31389129	7	38	from	population	986:995	arg1	evident					947:953	evident	947:953	evident	947:953	A. muciniphila influences the immune cell composition in mesenteric lymph nodes, as evident from an increased total B cell population, while reducing the total T cell and neutrophil populations.
31389129	6	39	theme	A.	759:760	arg1	administration					774:787	A. muciniphila administration	759:787	A. muciniphila administration	759:787	A. muciniphila administration lowers body weight and plasma total cholesterol and triglycerides levels.
31389129	8	40	theme	markers	1127:1133	arg1	expression					1098:1107	the expression	1094:1107	the expression of the activation markers MHCII on dendritic cells and CD86 on B cells	1094:1178	Importantly, A. muciniphila reduces the expression of the activation markers MHCII on dendritic cells and CD86 on B cells.
31389129	6	41	theme	triglycerides	841:853	arg1	levels					855:860	plasma total cholesterol and triglycerides levels	812:860	plasma total cholesterol and triglycerides levels	812:860	A. muciniphila administration lowers body weight and plasma total cholesterol and triglycerides levels.
31389129	10	42	theme	neointima	1383:1391	arg1	formation					1393:1401	neointima formation	1383:1401	neointima formation	1383:1401	Finally, although treatment with A. muciniphila improves lipid metabolism and immunity, it does not affect neointima formation or composition.
31389129	7	43	theme	lymph	931:935	arg1	nodes					937:941	mesenteric lymph nodes	920:941	mesenteric lymph nodes	920:941	A. muciniphila influences the immune cell composition in mesenteric lymph nodes, as evident from an increased total B cell population, while reducing the total T cell and neutrophil populations.
31389129	2	44	with	commensal	220:228	arg1	properties					253:262	anti-inflammatory properties	235:262	anti-inflammatory properties	235:262	SCOPE Akkermansia muciniphila (A. muciniphila) is an intestinal commensal with anti-inflammatory properties both in the intestine and other organs.
31389129	9	45	theme	whole	1211:1215	arg1	blood					1217:1221	whole blood	1211:1221	whole blood	1211:1221	A. muciniphila also increases whole blood ex vivo lipopolysaccharide-stimulated IL-10 release.
31389129	6	46	theme	total	819:823	arg1	cholesterol					825:835	plasma total cholesterol	812:835	plasma total cholesterol	812:835	A. muciniphila administration lowers body weight and plasma total cholesterol and triglycerides levels.
31389129	7	47	theme	cell	900:903	arg1	composition					905:915	the immune cell composition	889:915	the immune cell composition in mesenteric lymph nodes	889:941	A. muciniphila influences the immune cell composition in mesenteric lymph nodes, as evident from an increased total B cell population, while reducing the total T cell and neutrophil populations.
31389129	11	48	theme	hyperlipidemic	1584:1597	arg1	mice					1608:1611	hyperlipidemic E3L.CETP mice	1584:1611	hyperlipidemic E3L.CETP mice	1584:1611	CONCLUSIONS Four weeks of treatment with A. muciniphila exerts lipid-lowering and immunomodulatory effects, which are insufficient to inhibit neointima formation in hyperlipidemic E3L.CETP mice.
31389129	8	49	theme	B	1172:1172	arg1	cells					1174:1178	B cells	1172:1178	B cells	1172:1178	Importantly, A. muciniphila reduces the expression of the activation markers MHCII on dendritic cells and CD86 on B cells.
31389129	2	50	theme	SCOPE	156:160	arg1	commensal					220:228	an intestinal commensal	206:228	an intestinal commensal with anti-inflammatory properties both in the intestine and other organs	206:301	SCOPE Akkermansia muciniphila (A. muciniphila) is an intestinal commensal with anti-inflammatory properties both in the intestine and other organs.
31389129	2	50	theme	SCOPE	156:160	arg1	muciniphila					174:184	SCOPE Akkermansia muciniphila	156:184	SCOPE Akkermansia muciniphila (A. muciniphila)	156:201	SCOPE Akkermansia muciniphila (A. muciniphila) is an intestinal commensal with anti-inflammatory properties both in the intestine and other organs.
31389129	6	51	theme	body	796:799	arg1	weight					801:806	body weight	796:806	body weight	796:806	A. muciniphila administration lowers body weight and plasma total cholesterol and triglycerides levels.
31389129	5	52	theme	immune	686:691	arg1	parameters					693:702	immune parameters	686:702	immune parameters	686:702	METHODS AND RESULTS Hyperlipidemic male E3L.CETP mice are daily treated with 2 × 108 CFU A. muciniphila by oral gavage for 4 weeks and the effects are determined on plasma lipid levels, immune parameters, and cuff-induced neointima formation and composition.
31389129	9	53	theme	ex	1223:1224	arg1	release					1267:1273	ex vivo lipopolysaccharide-stimulated IL-10 release	1223:1273	ex vivo lipopolysaccharide-stimulated IL-10 release	1223:1273	A. muciniphila also increases whole blood ex vivo lipopolysaccharide-stimulated IL-10 release.
31389129	9	54	theme	lipopolysaccharide-stimulated	1231:1259	arg1	release					1267:1273	ex vivo lipopolysaccharide-stimulated IL-10 release	1223:1273	ex vivo lipopolysaccharide-stimulated IL-10 release	1223:1273	A. muciniphila also increases whole blood ex vivo lipopolysaccharide-stimulated IL-10 release.
31389129	5	55	theme	cuff-induced	709:720	arg1	formation					732:740	cuff-induced neointima formation	709:740	cuff-induced neointima formation	709:740	METHODS AND RESULTS Hyperlipidemic male E3L.CETP mice are daily treated with 2 × 108 CFU A. muciniphila by oral gavage for 4 weeks and the effects are determined on plasma lipid levels, immune parameters, and cuff-induced neointima formation and composition.
31389129	5	56	theme	plasma	665:670	arg1	levels					678:683	plasma lipid levels	665:683	plasma lipid levels	665:683	METHODS AND RESULTS Hyperlipidemic male E3L.CETP mice are daily treated with 2 × 108 CFU A. muciniphila by oral gavage for 4 weeks and the effects are determined on plasma lipid levels, immune parameters, and cuff-induced neointima formation and composition.
31389129	2	57	theme	other	290:294	arg1	organs					296:301	other organs	290:301	other organs	290:301	SCOPE Akkermansia muciniphila (A. muciniphila) is an intestinal commensal with anti-inflammatory properties both in the intestine and other organs.
31389129	5	58	theme	oral	607:610	arg1	gavage					612:617	oral gavage	607:617	oral gavage for 4 weeks	607:629	METHODS AND RESULTS Hyperlipidemic male E3L.CETP mice are daily treated with 2 × 108 CFU A. muciniphila by oral gavage for 4 weeks and the effects are determined on plasma lipid levels, immune parameters, and cuff-induced neointima formation and composition.
31389129	0	59	theme	Neointima	93:101	arg1	Formation					103:111	Neointima Formation	93:111	Neointima Formation in Hyperlipidemic APOE*3-Leiden	93:143	Akkermansia muciniphila Exerts Lipid-Lowering and Immunomodulatory Effects without Affecting Neointima Formation in Hyperlipidemic APOE*3-Leiden.
31389129	5	60	theme	male	535:538	arg1	mice					549:552	Hyperlipidemic male E3L.CETP mice	520:552	Hyperlipidemic male E3L.CETP mice	520:552	METHODS AND RESULTS Hyperlipidemic male E3L.CETP mice are daily treated with 2 × 108 CFU A. muciniphila by oral gavage for 4 weeks and the effects are determined on plasma lipid levels, immune parameters, and cuff-induced neointima formation and composition.
31389129	3	61	theme	administration	350:363	arg1	effects					334:340	the effects	330:340	the effects of oral administration of A. muciniphila on lipid metabolism, immunity, and cuff-induced neointima formation in hyperlipidemic APOE*3-Leiden (E3L)	330:487	The aim is to investigate the effects of oral administration of A. muciniphila on lipid metabolism, immunity, and cuff-induced neointima formation in hyperlipidemic APOE*3-Leiden (E3L).
31389129	11	62	theme	neointima	1561:1569	arg1	formation					1571:1579	neointima formation	1561:1579	neointima formation	1561:1579	CONCLUSIONS Four weeks of treatment with A. muciniphila exerts lipid-lowering and immunomodulatory effects, which are insufficient to inhibit neointima formation in hyperlipidemic E3L.CETP mice.
31389129	5	63	theme	neointima	722:730	arg1	formation					732:740	cuff-induced neointima formation	709:740	cuff-induced neointima formation	709:740	METHODS AND RESULTS Hyperlipidemic male E3L.CETP mice are daily treated with 2 × 108 CFU A. muciniphila by oral gavage for 4 weeks and the effects are determined on plasma lipid levels, immune parameters, and cuff-induced neointima formation and composition.
31389129	9	64	dep	ex	1223:1224	arg1	vivo					1226:1229	vivo	1226:1229	vivo	1226:1229	A. muciniphila also increases whole blood ex vivo lipopolysaccharide-stimulated IL-10 release.
31389129	8	65	from	MHCII	1135:1139	arg1	cells					1154:1158	dendritic cells	1144:1158	dendritic cells	1144:1158	Importantly, A. muciniphila reduces the expression of the activation markers MHCII on dendritic cells and CD86 on B cells.
31389129	0	66	theme	APOE	131:134	arg1	3-Leiden					136:143	Hyperlipidemic APOE*3-Leiden	116:143	Hyperlipidemic APOE*3-Leiden	116:143	Akkermansia muciniphila Exerts Lipid-Lowering and Immunomodulatory Effects without Affecting Neointima Formation in Hyperlipidemic APOE*3-Leiden.
31389129	7	67	theme	cell	981:984	arg1	population					986:995	an increased total B cell population	960:995	an increased total B cell population	960:995	A. muciniphila influences the immune cell composition in mesenteric lymph nodes, as evident from an increased total B cell population, while reducing the total T cell and neutrophil populations.
31389129	3	68	from	metabolism	392:401	arg1	3-Leiden					474:481	hyperlipidemic APOE*3-Leiden	454:481	hyperlipidemic APOE*3-Leiden (E3L)	454:487	The aim is to investigate the effects of oral administration of A. muciniphila on lipid metabolism, immunity, and cuff-induced neointima formation in hyperlipidemic APOE*3-Leiden (E3L).
31389129	3	68	from	metabolism	392:401	arg1	E3L					484:486	E3L	484:486	E3L	484:486	The aim is to investigate the effects of oral administration of A. muciniphila on lipid metabolism, immunity, and cuff-induced neointima formation in hyperlipidemic APOE*3-Leiden (E3L).
31389129	7	69	theme	total	973:977	arg1	population					986:995	an increased total B cell population	960:995	an increased total B cell population	960:995	A. muciniphila influences the immune cell composition in mesenteric lymph nodes, as evident from an increased total B cell population, while reducing the total T cell and neutrophil populations.
31389129	3	70	theme	APOE	469:472	arg1	3-Leiden					474:481	hyperlipidemic APOE*3-Leiden	454:481	hyperlipidemic APOE*3-Leiden (E3L)	454:487	The aim is to investigate the effects of oral administration of A. muciniphila on lipid metabolism, immunity, and cuff-induced neointima formation in hyperlipidemic APOE*3-Leiden (E3L).
31389129	3	70	theme	APOE	469:472	arg1	E3L					484:486	E3L	484:486	E3L	484:486	The aim is to investigate the effects of oral administration of A. muciniphila on lipid metabolism, immunity, and cuff-induced neointima formation in hyperlipidemic APOE*3-Leiden (E3L).
31389129	3	71	from	immunity	404:411	arg1	3-Leiden					474:481	hyperlipidemic APOE*3-Leiden	454:481	hyperlipidemic APOE*3-Leiden (E3L)	454:487	The aim is to investigate the effects of oral administration of A. muciniphila on lipid metabolism, immunity, and cuff-induced neointima formation in hyperlipidemic APOE*3-Leiden (E3L).
31389129	3	71	from	immunity	404:411	arg1	E3L					484:486	E3L	484:486	E3L	484:486	The aim is to investigate the effects of oral administration of A. muciniphila on lipid metabolism, immunity, and cuff-induced neointima formation in hyperlipidemic APOE*3-Leiden (E3L).
31389129	11	72	theme	immunomodulatory	1501:1516	arg1	effects					1518:1524	lipid-lowering and immunomodulatory effects	1482:1524	lipid-lowering and immunomodulatory effects	1482:1524	CONCLUSIONS Four weeks of treatment with A. muciniphila exerts lipid-lowering and immunomodulatory effects, which are insufficient to inhibit neointima formation in hyperlipidemic E3L.CETP mice.
31389129	0	73	theme	Lipid-Lowering	31:44	arg1	Effects					67:73	Lipid-Lowering and Immunomodulatory Effects	31:73	Lipid-Lowering and Immunomodulatory Effects	31:73	Akkermansia muciniphila Exerts Lipid-Lowering and Immunomodulatory Effects without Affecting Neointima Formation in Hyperlipidemic APOE*3-Leiden.
31389129	11	74	theme	lipid-lowering	1482:1495	arg1	effects					1518:1524	lipid-lowering and immunomodulatory effects	1482:1524	lipid-lowering and immunomodulatory effects	1482:1524	CONCLUSIONS Four weeks of treatment with A. muciniphila exerts lipid-lowering and immunomodulatory effects, which are insufficient to inhibit neointima formation in hyperlipidemic E3L.CETP mice.
31389129	0	75	theme	Immunomodulatory	50:65	arg1	Effects					67:73	Lipid-Lowering and Immunomodulatory Effects	31:73	Lipid-Lowering and Immunomodulatory Effects	31:73	Akkermansia muciniphila Exerts Lipid-Lowering and Immunomodulatory Effects without Affecting Neointima Formation in Hyperlipidemic APOE*3-Leiden.
31389129	3	76	theme	neointima	431:439	arg1	formation					441:449	cuff-induced neointima formation	418:449	cuff-induced neointima formation in hyperlipidemic APOE*3-Leiden (E3L)	418:487	The aim is to investigate the effects of oral administration of A. muciniphila on lipid metabolism, immunity, and cuff-induced neointima formation in hyperlipidemic APOE*3-Leiden (E3L).
31389129	6	77	theme	muciniphila	762:772	arg1	administration					774:787	A. muciniphila administration	759:787	A. muciniphila administration	759:787	A. muciniphila administration lowers body weight and plasma total cholesterol and triglycerides levels.
31389129	8	78	theme	activation	1116:1125	arg1	markers					1127:1133	the activation markers MHCII on dendritic cells and CD86	1112:1167	the activation markers MHCII on dendritic cells and CD86	1112:1167	Importantly, A. muciniphila reduces the expression of the activation markers MHCII on dendritic cells and CD86 on B cells.
31389129	8	78	theme	activation	1116:1125	arg1	CD86					1164:1167	CD86	1164:1167	CD86	1164:1167	Importantly, A. muciniphila reduces the expression of the activation markers MHCII on dendritic cells and CD86 on B cells.
31389129	8	78	theme	activation	1116:1125	arg1	MHCII					1135:1139	MHCII	1135:1139	MHCII on dendritic cells	1135:1158	Importantly, A. muciniphila reduces the expression of the activation markers MHCII on dendritic cells and CD86 on B cells.
31389129	11	79	with	treatment	1445:1453	arg1	muciniphila					1463:1473	A. muciniphila	1460:1473	A. muciniphila	1460:1473	CONCLUSIONS Four weeks of treatment with A. muciniphila exerts lipid-lowering and immunomodulatory effects, which are insufficient to inhibit neointima formation in hyperlipidemic E3L.CETP mice.
31389129	2	80	theme	intestinal	209:218	arg1	muciniphila					174:184	SCOPE Akkermansia muciniphila	156:184	SCOPE Akkermansia muciniphila (A. muciniphila)	156:201	SCOPE Akkermansia muciniphila (A. muciniphila) is an intestinal commensal with anti-inflammatory properties both in the intestine and other organs.
31389129	2	80	theme	intestinal	209:218	arg1	commensal					220:228	an intestinal commensal	206:228	an intestinal commensal with anti-inflammatory properties both in the intestine and other organs	206:301	SCOPE Akkermansia muciniphila (A. muciniphila) is an intestinal commensal with anti-inflammatory properties both in the intestine and other organs.
31389129	3	81	from	formation	441:449	arg1	3-Leiden					474:481	hyperlipidemic APOE*3-Leiden	454:481	hyperlipidemic APOE*3-Leiden (E3L)	454:487	The aim is to investigate the effects of oral administration of A. muciniphila on lipid metabolism, immunity, and cuff-induced neointima formation in hyperlipidemic APOE*3-Leiden (E3L).
31389129	3	81	from	formation	441:449	arg1	E3L					484:486	E3L	484:486	E3L	484:486	The aim is to investigate the effects of oral administration of A. muciniphila on lipid metabolism, immunity, and cuff-induced neointima formation in hyperlipidemic APOE*3-Leiden (E3L).
31389129	7	82	theme	mesenteric	920:929	arg1	nodes					937:941	mesenteric lymph nodes	920:941	mesenteric lymph nodes	920:941	A. muciniphila influences the immune cell composition in mesenteric lymph nodes, as evident from an increased total B cell population, while reducing the total T cell and neutrophil populations.
31389129	8	83	theme	dendritic	1144:1152	arg1	cells					1154:1158	dendritic cells	1144:1158	dendritic cells	1144:1158	Importantly, A. muciniphila reduces the expression of the activation markers MHCII on dendritic cells and CD86 on B cells.
31389129	8	84	from	CD86	1164:1167	arg1	cells					1154:1158	dendritic cells	1144:1158	dendritic cells	1144:1158	Importantly, A. muciniphila reduces the expression of the activation markers MHCII on dendritic cells and CD86 on B cells.
31389129	11	85	theme	CONCLUSIONS	1419:1429	arg1	weeks					1436:1440	CONCLUSIONS Four weeks	1419:1440	CONCLUSIONS Four weeks of treatment with A. muciniphila	1419:1473	CONCLUSIONS Four weeks of treatment with A. muciniphila exerts lipid-lowering and immunomodulatory effects, which are insufficient to inhibit neointima formation in hyperlipidemic E3L.CETP mice.
31426954	6	0	theme	mixed	1010:1014	arg1	polysaccharides					1016:1030	mixed polysaccharides	1010:1030	mixed polysaccharides	1010:1030	The present study demonstrated a potential strategy to characterize mixed polysaccharides without separation and purification.
31426954	3	1	theme	acid	458:461	arg1	hydrolysis					463:472	graded acid hydrolysis	451:472	graded acid hydrolysis	451:472	Disaccharides were gradually released by graded acid hydrolysis, separated with 3 kDa molecular weight cut-off membrane, and analyzed by HPLC-ESI-MSn.
31426954	4	2	theme	disaccharides	754:766	arg1	information					733:743	composition information	721:743	composition information of these disaccharides	721:766	Furthermore, the monosaccharide composition of the filtrate during the gradient acid hydrolysis process and the final retentate were also determined to provide composition information of these disaccharides.
31426954	2	3	theme	sinensis	371:378	arg1	polysaccharides					380:394	Cyclina sinensis polysaccharides	363:394	Cyclina sinensis polysaccharides	363:394	The present study aimed to develop a protocol that allowed for quick characterization of mixed polysaccharides by selecting Cyclina sinensis polysaccharides as test beds.
31426954	2	3	theme	sinensis	371:378	arg1	beds					404:407	test beds	399:407	test beds	399:407	The present study aimed to develop a protocol that allowed for quick characterization of mixed polysaccharides by selecting Cyclina sinensis polysaccharides as test beds.
31426954	4	4	theme	acid	641:644	arg1	process					657:663	the gradient acid hydrolysis process	628:663	the gradient acid hydrolysis process	628:663	Furthermore, the monosaccharide composition of the filtrate during the gradient acid hydrolysis process and the final retentate were also determined to provide composition information of these disaccharides.
31426954	0	5	theme	disaccharides	97:109	arg1	composition					82:92	the monosaccharide composition	63:92	the monosaccharide composition of disaccharides released by graded acid hydrolysis	63:144	A strategy to identify mixed polysaccharides through analyzing the monosaccharide composition of disaccharides released by graded acid hydrolysis.
31426954	4	6	theme	gradient	632:639	arg1	process					657:663	the gradient acid hydrolysis process	628:663	the gradient acid hydrolysis process	628:663	Furthermore, the monosaccharide composition of the filtrate during the gradient acid hydrolysis process and the final retentate were also determined to provide composition information of these disaccharides.
31426954	0	7	theme	graded	123:128	arg1	hydrolysis					135:144	graded acid hydrolysis	123:144	graded acid hydrolysis	123:144	A strategy to identify mixed polysaccharides through analyzing the monosaccharide composition of disaccharides released by graded acid hydrolysis.
31426954	6	8	theme	potential	975:983	arg1	strategy					985:992	a potential strategy	973:992	a potential strategy to characterize mixed polysaccharides without separation and purification	973:1066	The present study demonstrated a potential strategy to characterize mixed polysaccharides without separation and purification.
31426954	4	9	theme	final	673:677	arg1	retentate					679:687	the final retentate	669:687	the final retentate	669:687	Furthermore, the monosaccharide composition of the filtrate during the gradient acid hydrolysis process and the final retentate were also determined to provide composition information of these disaccharides.
31426954	1	10	theme	mixed	183:187	arg1	polysaccharides					189:203	mixed polysaccharides	183:203	mixed polysaccharides isolated from biological tissues	183:236	It is still a challenge to identify mixed polysaccharides isolated from biological tissues.
31426954	2	11	theme	mixed	328:332	arg1	polysaccharides					334:348	mixed polysaccharides	328:348	mixed polysaccharides	328:348	The present study aimed to develop a protocol that allowed for quick characterization of mixed polysaccharides by selecting Cyclina sinensis polysaccharides as test beds.
31426954	4	12	theme	composition	721:731	arg1	information					733:743	composition information	721:743	composition information of these disaccharides	721:766	Furthermore, the monosaccharide composition of the filtrate during the gradient acid hydrolysis process and the final retentate were also determined to provide composition information of these disaccharides.
31426954	5	13	theme	corresponding	880:892	arg1	fragments					916:924	corresponding repeated disaccharide fragments	880:924	corresponding repeated disaccharide fragments in C. sinensis	880:939	Thus, 6 disaccharides were detected and identified, indicating the existence of polysaccharides constructed of corresponding repeated disaccharide fragments in C. sinensis.
31426954	6	14	theme	present	946:952	arg1	study					954:958	The present study	942:958	The present study	942:958	The present study demonstrated a potential strategy to characterize mixed polysaccharides without separation and purification.
31426954	5	15	theme	repeated	894:901	arg1	fragments					916:924	corresponding repeated disaccharide fragments	880:924	corresponding repeated disaccharide fragments in C. sinensis	880:939	Thus, 6 disaccharides were detected and identified, indicating the existence of polysaccharides constructed of corresponding repeated disaccharide fragments in C. sinensis.
31426954	3	16	theme	molecular	496:504	arg1	weight					506:511	3 kDa molecular weight	490:511	3 kDa molecular weight cut-off membrane	490:528	Disaccharides were gradually released by graded acid hydrolysis, separated with 3 kDa molecular weight cut-off membrane, and analyzed by HPLC-ESI-MSn.
31426954	5	17	theme	disaccharide	903:914	arg1	fragments					916:924	corresponding repeated disaccharide fragments	880:924	corresponding repeated disaccharide fragments in C. sinensis	880:939	Thus, 6 disaccharides were detected and identified, indicating the existence of polysaccharides constructed of corresponding repeated disaccharide fragments in C. sinensis.
31426954	4	18	theme	filtrate	612:619	arg1	composition					593:603	the monosaccharide composition	574:603	the monosaccharide composition of the filtrate during the gradient acid hydrolysis process and the final retentate	574:687	Furthermore, the monosaccharide composition of the filtrate during the gradient acid hydrolysis process and the final retentate were also determined to provide composition information of these disaccharides.
31426954	0	19	theme	acid	130:133	arg1	hydrolysis					135:144	graded acid hydrolysis	123:144	graded acid hydrolysis	123:144	A strategy to identify mixed polysaccharides through analyzing the monosaccharide composition of disaccharides released by graded acid hydrolysis.
31426954	2	20	theme	quick	302:306	arg1	characterization					308:323	quick characterization	302:323	quick characterization of mixed polysaccharides	302:348	The present study aimed to develop a protocol that allowed for quick characterization of mixed polysaccharides by selecting Cyclina sinensis polysaccharides as test beds.
31426954	3	21	theme	weight	506:511	arg1	membrane					521:528	3 kDa molecular weight cut-off membrane	490:528	3 kDa molecular weight cut-off membrane	490:528	Disaccharides were gradually released by graded acid hydrolysis, separated with 3 kDa molecular weight cut-off membrane, and analyzed by HPLC-ESI-MSn.
31426954	5	22	theme	C.	929:930	arg1	sinensis					932:939	C. sinensis	929:939	C. sinensis	929:939	Thus, 6 disaccharides were detected and identified, indicating the existence of polysaccharides constructed of corresponding repeated disaccharide fragments in C. sinensis.
31426954	1	23	theme	biological	219:228	arg1	tissues					230:236	biological tissues	219:236	biological tissues	219:236	It is still a challenge to identify mixed polysaccharides isolated from biological tissues.
31426954	3	24	theme	graded	451:456	arg1	hydrolysis					463:472	graded acid hydrolysis	451:472	graded acid hydrolysis	451:472	Disaccharides were gradually released by graded acid hydrolysis, separated with 3 kDa molecular weight cut-off membrane, and analyzed by HPLC-ESI-MSn.
31426954	0	25	theme	mixed	23:27	arg1	polysaccharides					29:43	mixed polysaccharides	23:43	mixed polysaccharides	23:43	A strategy to identify mixed polysaccharides through analyzing the monosaccharide composition of disaccharides released by graded acid hydrolysis.
31426954	2	26	theme	present	243:249	arg1	study					251:255	The present study	239:255	The present study	239:255	The present study aimed to develop a protocol that allowed for quick characterization of mixed polysaccharides by selecting Cyclina sinensis polysaccharides as test beds.
31426954	3	27	theme	3 kDa	490:494	arg1	weight					506:511	3 kDa molecular weight	490:511	3 kDa molecular weight cut-off membrane	490:528	Disaccharides were gradually released by graded acid hydrolysis, separated with 3 kDa molecular weight cut-off membrane, and analyzed by HPLC-ESI-MSn.
31426954	5	28	theme	polysaccharides	849:863	arg1	existence					836:844	the existence	832:844	the existence of polysaccharides constructed of corresponding repeated disaccharide fragments in C. sinensis	832:939	Thus, 6 disaccharides were detected and identified, indicating the existence of polysaccharides constructed of corresponding repeated disaccharide fragments in C. sinensis.
31426954	2	29	theme	Cyclina	363:369	arg1	polysaccharides					380:394	Cyclina sinensis polysaccharides	363:394	Cyclina sinensis polysaccharides	363:394	The present study aimed to develop a protocol that allowed for quick characterization of mixed polysaccharides by selecting Cyclina sinensis polysaccharides as test beds.
31426954	2	29	theme	Cyclina	363:369	arg1	beds					404:407	test beds	399:407	test beds	399:407	The present study aimed to develop a protocol that allowed for quick characterization of mixed polysaccharides by selecting Cyclina sinensis polysaccharides as test beds.
31426954	4	30	theme	monosaccharide	578:591	arg1	composition					593:603	the monosaccharide composition	574:603	the monosaccharide composition of the filtrate during the gradient acid hydrolysis process and the final retentate	574:687	Furthermore, the monosaccharide composition of the filtrate during the gradient acid hydrolysis process and the final retentate were also determined to provide composition information of these disaccharides.
31426954	5	31	from	fragments	916:924	arg1	sinensis					932:939	C. sinensis	929:939	C. sinensis	929:939	Thus, 6 disaccharides were detected and identified, indicating the existence of polysaccharides constructed of corresponding repeated disaccharide fragments in C. sinensis.
31426954	1	32	attach	isolated	205:212	arg2	polysaccharides					189:203	mixed polysaccharides	183:203	mixed polysaccharides isolated from biological tissues	183:236	It is still a challenge to identify mixed polysaccharides isolated from biological tissues.
31426954	1	32	attach	isolated	205:212	arg1	tissues					230:236	biological tissues	219:236	biological tissues	219:236	It is still a challenge to identify mixed polysaccharides isolated from biological tissues.
31426954	3	33	theme	cut-off	513:519	arg1	membrane					521:528	3 kDa molecular weight cut-off membrane	490:528	3 kDa molecular weight cut-off membrane	490:528	Disaccharides were gradually released by graded acid hydrolysis, separated with 3 kDa molecular weight cut-off membrane, and analyzed by HPLC-ESI-MSn.
31426954	2	34	theme	polysaccharides	334:348	arg1	characterization					308:323	quick characterization	302:323	quick characterization of mixed polysaccharides	302:348	The present study aimed to develop a protocol that allowed for quick characterization of mixed polysaccharides by selecting Cyclina sinensis polysaccharides as test beds.
31426954	4	35	theme	hydrolysis	646:655	arg1	process					657:663	the gradient acid hydrolysis process	628:663	the gradient acid hydrolysis process	628:663	Furthermore, the monosaccharide composition of the filtrate during the gradient acid hydrolysis process and the final retentate were also determined to provide composition information of these disaccharides.
31426954	0	36	theme	monosaccharide	67:80	arg1	composition					82:92	the monosaccharide composition	63:92	the monosaccharide composition of disaccharides released by graded acid hydrolysis	63:144	A strategy to identify mixed polysaccharides through analyzing the monosaccharide composition of disaccharides released by graded acid hydrolysis.
31426954	4	37	theme	retentate	679:687	arg1	composition					593:603	the monosaccharide composition	574:603	the monosaccharide composition of the filtrate during the gradient acid hydrolysis process and the final retentate	574:687	Furthermore, the monosaccharide composition of the filtrate during the gradient acid hydrolysis process and the final retentate were also determined to provide composition information of these disaccharides.
31426954	2	38	theme	test	399:402	arg1	polysaccharides					380:394	Cyclina sinensis polysaccharides	363:394	Cyclina sinensis polysaccharides	363:394	The present study aimed to develop a protocol that allowed for quick characterization of mixed polysaccharides by selecting Cyclina sinensis polysaccharides as test beds.
31426954	2	38	theme	test	399:402	arg1	beds					404:407	test beds	399:407	test beds	399:407	The present study aimed to develop a protocol that allowed for quick characterization of mixed polysaccharides by selecting Cyclina sinensis polysaccharides as test beds.
29393155	5	0	theme	bioactivity	776:786	arg1	study					788:792	In vitro bioactivity study	767:792	In vitro bioactivity study in rat bone marrow-derived mesenchymal stem cells	767:842	In vitro bioactivity study in rat bone marrow-derived mesenchymal stem cells showed that the SIM-loaded scaffolds had a strong ability in accelerating cell proliferation and inducing osteogenic differentiation.
29393155	7	1	from	feasible	1219:1226	arg1	repair					1250:1255	bone repair	1245:1255	bone repair	1245:1255	The results of this study suggest that the SIM-loaded CS/HA scaffold is feasible and effective in bone repair and thus may provide a promising route for the treatment of critical-sized bone defects.
29393155	5	2	theme	cell	918:921	arg1	proliferation					923:935	cell proliferation	918:935	cell proliferation	918:935	In vitro bioactivity study in rat bone marrow-derived mesenchymal stem cells showed that the SIM-loaded scaffolds had a strong ability in accelerating cell proliferation and inducing osteogenic differentiation.
29393155	6	3	theme	defect	1032:1037	arg1	model					1039:1043	a rat calvarial defect model	1016:1043	a rat calvarial defect model	1016:1043	Moreover, an in vivo experiment using a rat calvarial defect model also documented that the SIM-loaded scaffolds had a remarkable effect on bone-promoting regeneration.
29393155	7	4	theme	study	1167:1171	arg1	results					1151:1157	The results	1147:1157	The results of this study	1147:1171	The results of this study suggest that the SIM-loaded CS/HA scaffold is feasible and effective in bone repair and thus may provide a promising route for the treatment of critical-sized bone defects.
29393155	3	5	from	effect	535:540	arg1	micro-architecture					563:580	micro-architecture	563:580	micro-architecture	563:580	The CS/HA weight ratio of 1:2 was selected by analyzing the effect of HA content on the micro-architecture, mechanical strength, and biocompatibility of the scaffold.
29393155	3	5	from	effect	535:540	arg1	strength					594:601	mechanical strength	583:601	mechanical strength	583:601	The CS/HA weight ratio of 1:2 was selected by analyzing the effect of HA content on the micro-architecture, mechanical strength, and biocompatibility of the scaffold.
29393155	3	5	from	effect	535:540	arg1	biocompatibility					608:623	biocompatibility	608:623	biocompatibility	608:623	The CS/HA weight ratio of 1:2 was selected by analyzing the effect of HA content on the micro-architecture, mechanical strength, and biocompatibility of the scaffold.
29393155	6	6	theme	bone-promoting	1118:1131	arg1	regeneration					1133:1144	bone-promoting regeneration	1118:1144	bone-promoting regeneration	1118:1144	Moreover, an in vivo experiment using a rat calvarial defect model also documented that the SIM-loaded scaffolds had a remarkable effect on bone-promoting regeneration.
29393155	6	7	theme	calvarial	1022:1030	arg1	model					1039:1043	a rat calvarial defect model	1016:1043	a rat calvarial defect model	1016:1043	Moreover, an in vivo experiment using a rat calvarial defect model also documented that the SIM-loaded scaffolds had a remarkable effect on bone-promoting regeneration.
29393155	3	8	theme	HA	545:546	arg1	content					548:554	HA content	545:554	HA content	545:554	The CS/HA weight ratio of 1:2 was selected by analyzing the effect of HA content on the micro-architecture, mechanical strength, and biocompatibility of the scaffold.
29393155	6	9	theme	SIM-loaded	1070:1079	arg1	scaffolds					1081:1089	the SIM-loaded scaffolds	1066:1089	the SIM-loaded scaffolds	1066:1089	Moreover, an in vivo experiment using a rat calvarial defect model also documented that the SIM-loaded scaffolds had a remarkable effect on bone-promoting regeneration.
29393155	2	10	theme	simvastatin	328:338	arg1	microspheres					358:369	simvastatin (SIM)-loaded PLGA microspheres	328:369	simvastatin (SIM)-loaded PLGA microspheres	328:369	Here, chitosan (CS)/nano-hydroxyapatite (HA) scaffolds containing simvastatin (SIM)-loaded PLGA microspheres were fabricated by combining a freeze-drying technique with a modified water-oil-water emulsion method.
29393155	6	11	theme	rat	1018:1020	arg1	model					1039:1043	a rat calvarial defect model	1016:1043	a rat calvarial defect model	1016:1043	Moreover, an in vivo experiment using a rat calvarial defect model also documented that the SIM-loaded scaffolds had a remarkable effect on bone-promoting regeneration.
29393155	7	12	theme	bone	1245:1248	arg1	repair					1250:1255	bone repair	1245:1255	bone repair	1245:1255	The results of this study suggest that the SIM-loaded CS/HA scaffold is feasible and effective in bone repair and thus may provide a promising route for the treatment of critical-sized bone defects.
29393155	2	13	theme	-loaded	345:351	arg1	microspheres					358:369	simvastatin (SIM)-loaded PLGA microspheres	328:369	simvastatin (SIM)-loaded PLGA microspheres	328:369	Here, chitosan (CS)/nano-hydroxyapatite (HA) scaffolds containing simvastatin (SIM)-loaded PLGA microspheres were fabricated by combining a freeze-drying technique with a modified water-oil-water emulsion method.
29393155	7	14	theme	promising	1280:1288	arg1	route					1290:1294	a promising route	1278:1294	a promising route for the treatment of critical-sized bone defects	1278:1343	The results of this study suggest that the SIM-loaded CS/HA scaffold is feasible and effective in bone repair and thus may provide a promising route for the treatment of critical-sized bone defects.
29393155	2	15	theme	emulsion	458:465	arg1	method					467:472	a modified water-oil-water emulsion method	431:472	a modified water-oil-water emulsion method	431:472	Here, chitosan (CS)/nano-hydroxyapatite (HA) scaffolds containing simvastatin (SIM)-loaded PLGA microspheres were fabricated by combining a freeze-drying technique with a modified water-oil-water emulsion method.
29393155	5	16	theme	osteogenic	950:959	arg1	differentiation					961:975	osteogenic differentiation	950:975	osteogenic differentiation	950:975	In vitro bioactivity study in rat bone marrow-derived mesenchymal stem cells showed that the SIM-loaded scaffolds had a strong ability in accelerating cell proliferation and inducing osteogenic differentiation.
29393155	1	17	theme	effective	205:213	arg1	combination					128:138	The combination	124:138	The combination of bone tissue scaffolds with osteogenic induction factors	124:197	The combination of bone tissue scaffolds with osteogenic induction factors is an effective strategy to facilitate bone healing processes.
29393155	1	17	theme	effective	205:213	arg1	strategy					215:222	an effective strategy	202:222	an effective strategy to facilitate bone healing processes	202:259	The combination of bone tissue scaffolds with osteogenic induction factors is an effective strategy to facilitate bone healing processes.
29393155	5	18	theme	marrow-derived	806:819	arg1	cells					838:842	rat bone marrow-derived mesenchymal stem cells	797:842	rat bone marrow-derived mesenchymal stem cells	797:842	In vitro bioactivity study in rat bone marrow-derived mesenchymal stem cells showed that the SIM-loaded scaffolds had a strong ability in accelerating cell proliferation and inducing osteogenic differentiation.
29393155	5	19	theme	mesenchymal	821:831	arg1	cells					838:842	rat bone marrow-derived mesenchymal stem cells	797:842	rat bone marrow-derived mesenchymal stem cells	797:842	In vitro bioactivity study in rat bone marrow-derived mesenchymal stem cells showed that the SIM-loaded scaffolds had a strong ability in accelerating cell proliferation and inducing osteogenic differentiation.
29393155	5	20	theme	stem	833:836	arg1	cells					838:842	rat bone marrow-derived mesenchymal stem cells	797:842	rat bone marrow-derived mesenchymal stem cells	797:842	In vitro bioactivity study in rat bone marrow-derived mesenchymal stem cells showed that the SIM-loaded scaffolds had a strong ability in accelerating cell proliferation and inducing osteogenic differentiation.
29393155	3	21	theme	weight	485:490	arg1	ratio					492:496	The CS/HA weight ratio	475:496	The CS/HA weight ratio of 1:2	475:503	The CS/HA weight ratio of 1:2 was selected by analyzing the effect of HA content on the micro-architecture, mechanical strength, and biocompatibility of the scaffold.
29393155	0	22	theme	Chitosan/Nano-Hydroxyapatite	7:34	arg1	Scaffolds					46:54	Porous Chitosan/Nano-Hydroxyapatite Composite Scaffolds	0:54	Porous Chitosan/Nano-Hydroxyapatite Composite Scaffolds	0:54	Porous Chitosan/Nano-Hydroxyapatite Composite Scaffolds Incorporating Simvastatin-Loaded PLGA Microspheres for Bone Repair.
29393155	6	23	contain	had	1091:1093	arg1	scaffolds					1081:1089	the SIM-loaded scaffolds	1066:1089	the SIM-loaded scaffolds	1066:1089	Moreover, an in vivo experiment using a rat calvarial defect model also documented that the SIM-loaded scaffolds had a remarkable effect on bone-promoting regeneration.
29393155	6	23	contain	had	1091:1093	arg2	effect					1108:1113	a remarkable effect	1095:1113	a remarkable effect	1095:1113	Moreover, an in vivo experiment using a rat calvarial defect model also documented that the SIM-loaded scaffolds had a remarkable effect on bone-promoting regeneration.
29393155	7	24	theme	critical-sized	1317:1330	arg1	defects					1337:1343	critical-sized bone defects	1317:1343	critical-sized bone defects	1317:1343	The results of this study suggest that the SIM-loaded CS/HA scaffold is feasible and effective in bone repair and thus may provide a promising route for the treatment of critical-sized bone defects.
29393155	5	25	theme	In	767:768	arg1	study					788:792	In vitro bioactivity study	767:792	In vitro bioactivity study in rat bone marrow-derived mesenchymal stem cells	767:842	In vitro bioactivity study in rat bone marrow-derived mesenchymal stem cells showed that the SIM-loaded scaffolds had a strong ability in accelerating cell proliferation and inducing osteogenic differentiation.
29393155	6	26	theme	remarkable	1097:1106	arg1	effect					1108:1113	a remarkable effect	1095:1113	a remarkable effect	1095:1113	Moreover, an in vivo experiment using a rat calvarial defect model also documented that the SIM-loaded scaffolds had a remarkable effect on bone-promoting regeneration.
29393155	5	27	theme	strong	887:892	arg1	ability					894:900	a strong ability	885:900	a strong ability	885:900	In vitro bioactivity study in rat bone marrow-derived mesenchymal stem cells showed that the SIM-loaded scaffolds had a strong ability in accelerating cell proliferation and inducing osteogenic differentiation.
29393155	0	28	theme	Porous	0:5	arg1	Scaffolds					46:54	Porous Chitosan/Nano-Hydroxyapatite Composite Scaffolds	0:54	Porous Chitosan/Nano-Hydroxyapatite Composite Scaffolds	0:54	Porous Chitosan/Nano-Hydroxyapatite Composite Scaffolds Incorporating Simvastatin-Loaded PLGA Microspheres for Bone Repair.
29393155	7	29	theme	SIM-loaded	1190:1199	arg1	scaffold					1207:1214	the SIM-loaded CS/HA scaffold	1186:1214	the SIM-loaded CS/HA scaffold	1186:1214	The results of this study suggest that the SIM-loaded CS/HA scaffold is feasible and effective in bone repair and thus may provide a promising route for the treatment of critical-sized bone defects.
29393155	7	29	theme	SIM-loaded	1190:1199	arg1	feasible					1219:1226	feasible	1219:1226	feasible	1219:1226	The results of this study suggest that the SIM-loaded CS/HA scaffold is feasible and effective in bone repair and thus may provide a promising route for the treatment of critical-sized bone defects.
29393155	2	30	theme	freeze-drying	402:414	arg1	technique					416:424	a freeze-drying technique	400:424	a freeze-drying technique	400:424	Here, chitosan (CS)/nano-hydroxyapatite (HA) scaffolds containing simvastatin (SIM)-loaded PLGA microspheres were fabricated by combining a freeze-drying technique with a modified water-oil-water emulsion method.
29393155	7	31	theme	CS/HA	1201:1205	arg1	scaffold					1207:1214	the SIM-loaded CS/HA scaffold	1186:1214	the SIM-loaded CS/HA scaffold	1186:1214	The results of this study suggest that the SIM-loaded CS/HA scaffold is feasible and effective in bone repair and thus may provide a promising route for the treatment of critical-sized bone defects.
29393155	7	31	theme	CS/HA	1201:1205	arg1	feasible					1219:1226	feasible	1219:1226	feasible	1219:1226	The results of this study suggest that the SIM-loaded CS/HA scaffold is feasible and effective in bone repair and thus may provide a promising route for the treatment of critical-sized bone defects.
29393155	5	32	contain	had	881:883	arg2	ability					894:900	a strong ability	885:900	a strong ability	885:900	In vitro bioactivity study in rat bone marrow-derived mesenchymal stem cells showed that the SIM-loaded scaffolds had a strong ability in accelerating cell proliferation and inducing osteogenic differentiation.
29393155	5	32	contain	had	881:883	arg1	scaffolds					871:879	the SIM-loaded scaffolds	856:879	the SIM-loaded scaffolds	856:879	In vitro bioactivity study in rat bone marrow-derived mesenchymal stem cells showed that the SIM-loaded scaffolds had a strong ability in accelerating cell proliferation and inducing osteogenic differentiation.
29393155	3	33	theme	mechanical	583:592	arg1	strength					594:601	mechanical strength	583:601	mechanical strength	583:601	The CS/HA weight ratio of 1:2 was selected by analyzing the effect of HA content on the micro-architecture, mechanical strength, and biocompatibility of the scaffold.
29393155	1	34	theme	bone	238:241	arg1	processes					251:259	bone healing processes	238:259	bone healing processes	238:259	The combination of bone tissue scaffolds with osteogenic induction factors is an effective strategy to facilitate bone healing processes.
29393155	1	35	with	combination	128:138	arg1	factors					191:197	osteogenic induction factors	170:197	osteogenic induction factors	170:197	The combination of bone tissue scaffolds with osteogenic induction factors is an effective strategy to facilitate bone healing processes.
29393155	1	36	theme	bone	143:146	arg1	scaffolds					155:163	bone tissue scaffolds	143:163	bone tissue scaffolds	143:163	The combination of bone tissue scaffolds with osteogenic induction factors is an effective strategy to facilitate bone healing processes.
29393155	1	37	theme	healing	243:249	arg1	processes					251:259	bone healing processes	238:259	bone healing processes	238:259	The combination of bone tissue scaffolds with osteogenic induction factors is an effective strategy to facilitate bone healing processes.
29393155	0	38	theme	Simvastatin-Loaded	70:87	arg1	Microspheres					94:105	Simvastatin-Loaded PLGA Microspheres	70:105	Simvastatin-Loaded PLGA Microspheres	70:105	Porous Chitosan/Nano-Hydroxyapatite Composite Scaffolds Incorporating Simvastatin-Loaded PLGA Microspheres for Bone Repair.
29393155	2	39	contain	containing	317:326	arg1	scaffolds					307:315	chitosan (CS)/nano-hydroxyapatite (HA) scaffolds	268:315	chitosan (CS)/nano-hydroxyapatite (HA) scaffolds containing simvastatin (SIM)-loaded PLGA microspheres	268:369	Here, chitosan (CS)/nano-hydroxyapatite (HA) scaffolds containing simvastatin (SIM)-loaded PLGA microspheres were fabricated by combining a freeze-drying technique with a modified water-oil-water emulsion method.
29393155	2	39	contain	containing	317:326	arg1	HA					303:304	HA	303:304	HA	303:304	Here, chitosan (CS)/nano-hydroxyapatite (HA) scaffolds containing simvastatin (SIM)-loaded PLGA microspheres were fabricated by combining a freeze-drying technique with a modified water-oil-water emulsion method.
29393155	2	39	contain	containing	317:326	arg2	microspheres					358:369	simvastatin (SIM)-loaded PLGA microspheres	328:369	simvastatin (SIM)-loaded PLGA microspheres	328:369	Here, chitosan (CS)/nano-hydroxyapatite (HA) scaffolds containing simvastatin (SIM)-loaded PLGA microspheres were fabricated by combining a freeze-drying technique with a modified water-oil-water emulsion method.
29393155	5	40	dep	In	767:768	arg1	vitro					770:774	vitro	770:774	vitro	770:774	In vitro bioactivity study in rat bone marrow-derived mesenchymal stem cells showed that the SIM-loaded scaffolds had a strong ability in accelerating cell proliferation and inducing osteogenic differentiation.
29393155	5	41	theme	SIM-loaded	860:869	arg1	scaffolds					871:879	the SIM-loaded scaffolds	856:879	the SIM-loaded scaffolds	856:879	In vitro bioactivity study in rat bone marrow-derived mesenchymal stem cells showed that the SIM-loaded scaffolds had a strong ability in accelerating cell proliferation and inducing osteogenic differentiation.
29393155	6	42	theme	in	991:992	arg1	experiment					999:1008	an in vivo experiment	988:1008	an in vivo experiment using a rat calvarial defect model	988:1043	Moreover, an in vivo experiment using a rat calvarial defect model also documented that the SIM-loaded scaffolds had a remarkable effect on bone-promoting regeneration.
29393155	2	43	theme	water-oil-water	442:456	arg1	method					467:472	a modified water-oil-water emulsion method	431:472	a modified water-oil-water emulsion method	431:472	Here, chitosan (CS)/nano-hydroxyapatite (HA) scaffolds containing simvastatin (SIM)-loaded PLGA microspheres were fabricated by combining a freeze-drying technique with a modified water-oil-water emulsion method.
29393155	3	44	theme	CS/HA	479:483	arg1	ratio					492:496	The CS/HA weight ratio	475:496	The CS/HA weight ratio of 1:2	475:503	The CS/HA weight ratio of 1:2 was selected by analyzing the effect of HA content on the micro-architecture, mechanical strength, and biocompatibility of the scaffold.
29393155	5	45	theme	rat	797:799	arg1	cells					838:842	rat bone marrow-derived mesenchymal stem cells	797:842	rat bone marrow-derived mesenchymal stem cells	797:842	In vitro bioactivity study in rat bone marrow-derived mesenchymal stem cells showed that the SIM-loaded scaffolds had a strong ability in accelerating cell proliferation and inducing osteogenic differentiation.
29393155	4	46	theme	sustained	731:739	arg1	manner					741:746	a sustained manner	729:746	a sustained manner	729:746	Drug release kinetics showed that the SIM encapsulated in the scaffold was released in a sustained manner for up to 30 days.
29393155	3	47	theme	scaffold	632:639	arg1	micro-architecture					563:580	micro-architecture	563:580	micro-architecture	563:580	The CS/HA weight ratio of 1:2 was selected by analyzing the effect of HA content on the micro-architecture, mechanical strength, and biocompatibility of the scaffold.
29393155	3	47	theme	scaffold	632:639	arg1	strength					594:601	mechanical strength	583:601	mechanical strength	583:601	The CS/HA weight ratio of 1:2 was selected by analyzing the effect of HA content on the micro-architecture, mechanical strength, and biocompatibility of the scaffold.
29393155	3	47	theme	scaffold	632:639	arg1	biocompatibility					608:623	biocompatibility	608:623	biocompatibility	608:623	The CS/HA weight ratio of 1:2 was selected by analyzing the effect of HA content on the micro-architecture, mechanical strength, and biocompatibility of the scaffold.
29393155	2	48	theme	modified	433:440	arg1	method					467:472	a modified water-oil-water emulsion method	431:472	a modified water-oil-water emulsion method	431:472	Here, chitosan (CS)/nano-hydroxyapatite (HA) scaffolds containing simvastatin (SIM)-loaded PLGA microspheres were fabricated by combining a freeze-drying technique with a modified water-oil-water emulsion method.
29393155	5	49	theme	bone	801:804	arg1	cells					838:842	rat bone marrow-derived mesenchymal stem cells	797:842	rat bone marrow-derived mesenchymal stem cells	797:842	In vitro bioactivity study in rat bone marrow-derived mesenchymal stem cells showed that the SIM-loaded scaffolds had a strong ability in accelerating cell proliferation and inducing osteogenic differentiation.
29393155	1	50	theme	tissue	148:153	arg1	scaffolds					155:163	bone tissue scaffolds	143:163	bone tissue scaffolds	143:163	The combination of bone tissue scaffolds with osteogenic induction factors is an effective strategy to facilitate bone healing processes.
29393155	0	51	theme	PLGA	89:92	arg1	Microspheres					94:105	Simvastatin-Loaded PLGA Microspheres	70:105	Simvastatin-Loaded PLGA Microspheres	70:105	Porous Chitosan/Nano-Hydroxyapatite Composite Scaffolds Incorporating Simvastatin-Loaded PLGA Microspheres for Bone Repair.
29393155	7	52	from	repair	1250:1255	arg1	scaffold					1207:1214	the SIM-loaded CS/HA scaffold	1186:1214	the SIM-loaded CS/HA scaffold	1186:1214	The results of this study suggest that the SIM-loaded CS/HA scaffold is feasible and effective in bone repair and thus may provide a promising route for the treatment of critical-sized bone defects.
29393155	7	52	from	repair	1250:1255	arg1	feasible					1219:1226	feasible	1219:1226	feasible	1219:1226	The results of this study suggest that the SIM-loaded CS/HA scaffold is feasible and effective in bone repair and thus may provide a promising route for the treatment of critical-sized bone defects.
29393155	6	53	dep	in	991:992	arg1	vivo					994:997	vivo	994:997	vivo	994:997	Moreover, an in vivo experiment using a rat calvarial defect model also documented that the SIM-loaded scaffolds had a remarkable effect on bone-promoting regeneration.
29393155	1	54	theme	scaffolds	155:163	arg1	combination					128:138	The combination	124:138	The combination of bone tissue scaffolds with osteogenic induction factors	124:197	The combination of bone tissue scaffolds with osteogenic induction factors is an effective strategy to facilitate bone healing processes.
29393155	1	54	theme	scaffolds	155:163	arg1	strategy					215:222	an effective strategy	202:222	an effective strategy to facilitate bone healing processes	202:259	The combination of bone tissue scaffolds with osteogenic induction factors is an effective strategy to facilitate bone healing processes.
29393155	5	55	from	study	788:792	arg1	cells					838:842	rat bone marrow-derived mesenchymal stem cells	797:842	rat bone marrow-derived mesenchymal stem cells	797:842	In vitro bioactivity study in rat bone marrow-derived mesenchymal stem cells showed that the SIM-loaded scaffolds had a strong ability in accelerating cell proliferation and inducing osteogenic differentiation.
29393155	5	56	link	marrow-derived	806:819	arg1	cells					838:842	rat bone marrow-derived mesenchymal stem cells	797:842	rat bone marrow-derived mesenchymal stem cells	797:842	In vitro bioactivity study in rat bone marrow-derived mesenchymal stem cells showed that the SIM-loaded scaffolds had a strong ability in accelerating cell proliferation and inducing osteogenic differentiation.
29393155	2	57	theme	PLGA	353:356	arg1	microspheres					358:369	simvastatin (SIM)-loaded PLGA microspheres	328:369	simvastatin (SIM)-loaded PLGA microspheres	328:369	Here, chitosan (CS)/nano-hydroxyapatite (HA) scaffolds containing simvastatin (SIM)-loaded PLGA microspheres were fabricated by combining a freeze-drying technique with a modified water-oil-water emulsion method.
29393155	4	58	theme	Drug	642:645	arg1	kinetics					655:662	Drug release kinetics	642:662	Drug release kinetics	642:662	Drug release kinetics showed that the SIM encapsulated in the scaffold was released in a sustained manner for up to 30 days.
29393155	2	59	theme	/nano-hydroxyapatite	281:300	arg1	scaffolds					307:315	chitosan (CS)/nano-hydroxyapatite (HA) scaffolds	268:315	chitosan (CS)/nano-hydroxyapatite (HA) scaffolds containing simvastatin (SIM)-loaded PLGA microspheres	268:369	Here, chitosan (CS)/nano-hydroxyapatite (HA) scaffolds containing simvastatin (SIM)-loaded PLGA microspheres were fabricated by combining a freeze-drying technique with a modified water-oil-water emulsion method.
29393155	2	59	theme	/nano-hydroxyapatite	281:300	arg1	HA					303:304	HA	303:304	HA	303:304	Here, chitosan (CS)/nano-hydroxyapatite (HA) scaffolds containing simvastatin (SIM)-loaded PLGA microspheres were fabricated by combining a freeze-drying technique with a modified water-oil-water emulsion method.
29393155	1	60	theme	osteogenic	170:179	arg1	factors					191:197	osteogenic induction factors	170:197	osteogenic induction factors	170:197	The combination of bone tissue scaffolds with osteogenic induction factors is an effective strategy to facilitate bone healing processes.
29393155	4	61	dep	30	758:759	arg1	to					755:756	to	755:756	to	755:756	Drug release kinetics showed that the SIM encapsulated in the scaffold was released in a sustained manner for up to 30 days.
29393155	0	62	theme	Bone	111:114	arg1	Repair					116:121	Bone Repair	111:121	Bone Repair	111:121	Porous Chitosan/Nano-Hydroxyapatite Composite Scaffolds Incorporating Simvastatin-Loaded PLGA Microspheres for Bone Repair.
29393155	7	63	theme	bone	1332:1335	arg1	defects					1337:1343	critical-sized bone defects	1317:1343	critical-sized bone defects	1317:1343	The results of this study suggest that the SIM-loaded CS/HA scaffold is feasible and effective in bone repair and thus may provide a promising route for the treatment of critical-sized bone defects.
29393155	3	64	theme	1:2	501:503	arg1	ratio					492:496	The CS/HA weight ratio	475:496	The CS/HA weight ratio of 1:2	475:503	The CS/HA weight ratio of 1:2 was selected by analyzing the effect of HA content on the micro-architecture, mechanical strength, and biocompatibility of the scaffold.
29393155	1	65	theme	induction	181:189	arg1	factors					191:197	osteogenic induction factors	170:197	osteogenic induction factors	170:197	The combination of bone tissue scaffolds with osteogenic induction factors is an effective strategy to facilitate bone healing processes.
29393155	7	66	theme	defects	1337:1343	arg1	treatment					1304:1312	the treatment	1300:1312	the treatment of critical-sized bone defects	1300:1343	The results of this study suggest that the SIM-loaded CS/HA scaffold is feasible and effective in bone repair and thus may provide a promising route for the treatment of critical-sized bone defects.
29393155	0	67	theme	Composite	36:44	arg1	Scaffolds					46:54	Porous Chitosan/Nano-Hydroxyapatite Composite Scaffolds	0:54	Porous Chitosan/Nano-Hydroxyapatite Composite Scaffolds	0:54	Porous Chitosan/Nano-Hydroxyapatite Composite Scaffolds Incorporating Simvastatin-Loaded PLGA Microspheres for Bone Repair.
29393155	3	68	theme	content	548:554	arg1	effect					535:540	the effect	531:540	the effect of HA content on the micro-architecture, mechanical strength, and biocompatibility of the scaffold	531:639	The CS/HA weight ratio of 1:2 was selected by analyzing the effect of HA content on the micro-architecture, mechanical strength, and biocompatibility of the scaffold.
29393155	4	69	theme	release	647:653	arg1	kinetics					655:662	Drug release kinetics	642:662	Drug release kinetics	642:662	Drug release kinetics showed that the SIM encapsulated in the scaffold was released in a sustained manner for up to 30 days.
30564225	8	0	theme	bacteria-mediated	2087:2103	arg1	diet					2105:2108	intestinal bacteria-mediated diet	2076:2108	intestinal bacteria-mediated diet on diseases	2076:2120	Our study shed a new strategy to explore the relationship among disease phenotypes (D), intestinal microbiota (I), enzymes (E) and traits of metabolism (T) named as "DIET," which can provide a reference for further study of the mechanism in regulation of intestinal bacteria-mediated diet on diseases.
30564225	8	1	from	mechanism	2049:2057	arg1	regulation					2062:2071	regulation	2062:2071	regulation of intestinal bacteria-mediated diet on diseases	2062:2120	Our study shed a new strategy to explore the relationship among disease phenotypes (D), intestinal microbiota (I), enzymes (E) and traits of metabolism (T) named as "DIET," which can provide a reference for further study of the mechanism in regulation of intestinal bacteria-mediated diet on diseases.
30564225	3	2	from	differences	609:619	arg1	constipation					582:593	constipation	582:593	constipation	582:593	To gain insight into the key role of intestinal microbiota in constipation and metabolic differences caused by isoflavones deficiency, we observed a significant decrease in fecal pellet numbers, fecal water content, intestinal transit rate together with the serum concentrations of substance P (SP) and vasoactive intestinal peptide (VIP) in NISO group, compared with those in the ISO group.
30564225	3	3	theme	pellet	699:704	arg1	numbers					706:712	fecal pellet numbers	693:712	fecal pellet numbers	693:712	To gain insight into the key role of intestinal microbiota in constipation and metabolic differences caused by isoflavones deficiency, we observed a significant decrease in fecal pellet numbers, fecal water content, intestinal transit rate together with the serum concentrations of substance P (SP) and vasoactive intestinal peptide (VIP) in NISO group, compared with those in the ISO group.
30564225	5	4	theme	diet	1419:1422	arg1	intake					1424:1429	non-isoflavones diet intake	1403:1429	non-isoflavones diet intake	1403:1429	In addition, Lactobacillus, produceing β-glucosidase which contribute to biotransform glycosides into aglycons and exert the bioactivities consequently, was decreased after non-isoflavones diet intake.
30564225	7	5	theme	β-glucosidase-producing	1787:1809	arg1	bacteria					1811:1818	β-glucosidase-producing bacteria	1787:1818	β-glucosidase-producing bacteria	1787:1818	Taken together, the findings suggested that the changes in the dietary components could alter the biochemical state of rats, which may be triggered by the abnormal modifications facilitated by β-glucosidase-producing bacteria.
30564225	4	6	theme	phyla	1055:1059	arg1	total					1044:1048	a total	1042:1048	a total of 5 phyla and 21 genera	1042:1073	Following 16S rRNA compositional sequencing, results excluded the changes in intestinal microbiota over time and highlighted that a total of 5 phyla and 21 genera changed significantly, among which Firmicutes, Bacteroidetes, Blautia, Prevotella, Lactobacillus and Bifidobacterium were closely related to constipation.
30564225	6	7	theme	metabolism	1520:1529	arg1	pathway					1485:1491	the pathway	1481:1491	the pathway of glycan biosynthesis and metabolism	1481:1529	Meanwhile, predicted metagenomics indicated that the pathway of glycan biosynthesis and metabolism was markedly down-regulated after non-isoflavones diet intake.
30564225	3	8	theme	water	721:725	arg1	content					727:733	fecal water content	715:733	fecal water content	715:733	To gain insight into the key role of intestinal microbiota in constipation and metabolic differences caused by isoflavones deficiency, we observed a significant decrease in fecal pellet numbers, fecal water content, intestinal transit rate together with the serum concentrations of substance P (SP) and vasoactive intestinal peptide (VIP) in NISO group, compared with those in the ISO group.
30564225	3	8	theme	water	721:725	arg1	decrease					681:688	a significant decrease	667:688	a significant decrease in fecal pellet numbers	667:712	To gain insight into the key role of intestinal microbiota in constipation and metabolic differences caused by isoflavones deficiency, we observed a significant decrease in fecal pellet numbers, fecal water content, intestinal transit rate together with the serum concentrations of substance P (SP) and vasoactive intestinal peptide (VIP) in NISO group, compared with those in the ISO group.
30564225	6	9	theme	biosynthesis	1503:1514	arg1	pathway					1485:1491	the pathway	1481:1491	the pathway of glycan biosynthesis and metabolism	1481:1529	Meanwhile, predicted metagenomics indicated that the pathway of glycan biosynthesis and metabolism was markedly down-regulated after non-isoflavones diet intake.
30564225	7	10	theme	abnormal	1749:1756	arg1	modifications					1758:1770	the abnormal modifications	1745:1770	the abnormal modifications facilitated by β-glucosidase-producing bacteria	1745:1818	Taken together, the findings suggested that the changes in the dietary components could alter the biochemical state of rats, which may be triggered by the abnormal modifications facilitated by β-glucosidase-producing bacteria.
30564225	3	11	theme	transit	747:753	arg1	decrease					681:688	a significant decrease	667:688	a significant decrease in fecal pellet numbers	667:712	To gain insight into the key role of intestinal microbiota in constipation and metabolic differences caused by isoflavones deficiency, we observed a significant decrease in fecal pellet numbers, fecal water content, intestinal transit rate together with the serum concentrations of substance P (SP) and vasoactive intestinal peptide (VIP) in NISO group, compared with those in the ISO group.
30564225	3	11	theme	transit	747:753	arg1	rate					755:758	intestinal transit rate	736:758	intestinal transit rate	736:758	To gain insight into the key role of intestinal microbiota in constipation and metabolic differences caused by isoflavones deficiency, we observed a significant decrease in fecal pellet numbers, fecal water content, intestinal transit rate together with the serum concentrations of substance P (SP) and vasoactive intestinal peptide (VIP) in NISO group, compared with those in the ISO group.
30564225	3	12	theme	intestinal	557:566	arg1	microbiota					568:577	intestinal microbiota	557:577	intestinal microbiota	557:577	To gain insight into the key role of intestinal microbiota in constipation and metabolic differences caused by isoflavones deficiency, we observed a significant decrease in fecal pellet numbers, fecal water content, intestinal transit rate together with the serum concentrations of substance P (SP) and vasoactive intestinal peptide (VIP) in NISO group, compared with those in the ISO group.
30564225	3	13	theme	intestinal	736:745	arg1	decrease					681:688	a significant decrease	667:688	a significant decrease in fecal pellet numbers	667:712	To gain insight into the key role of intestinal microbiota in constipation and metabolic differences caused by isoflavones deficiency, we observed a significant decrease in fecal pellet numbers, fecal water content, intestinal transit rate together with the serum concentrations of substance P (SP) and vasoactive intestinal peptide (VIP) in NISO group, compared with those in the ISO group.
30564225	3	13	theme	intestinal	736:745	arg1	rate					755:758	intestinal transit rate	736:758	intestinal transit rate	736:758	To gain insight into the key role of intestinal microbiota in constipation and metabolic differences caused by isoflavones deficiency, we observed a significant decrease in fecal pellet numbers, fecal water content, intestinal transit rate together with the serum concentrations of substance P (SP) and vasoactive intestinal peptide (VIP) in NISO group, compared with those in the ISO group.
30564225	3	14	from	decrease	681:688	arg1	numbers					706:712	fecal pellet numbers	693:712	fecal pellet numbers	693:712	To gain insight into the key role of intestinal microbiota in constipation and metabolic differences caused by isoflavones deficiency, we observed a significant decrease in fecal pellet numbers, fecal water content, intestinal transit rate together with the serum concentrations of substance P (SP) and vasoactive intestinal peptide (VIP) in NISO group, compared with those in the ISO group.
30564225	8	15	theme	disease	1885:1891	arg1	D					1905:1905	D	1905:1905	D	1905:1905	Our study shed a new strategy to explore the relationship among disease phenotypes (D), intestinal microbiota (I), enzymes (E) and traits of metabolism (T) named as "DIET," which can provide a reference for further study of the mechanism in regulation of intestinal bacteria-mediated diet on diseases.
30564225	8	15	theme	disease	1885:1891	arg1	phenotypes					1893:1902	disease phenotypes	1885:1902	disease phenotypes (D)	1885:1906	Our study shed a new strategy to explore the relationship among disease phenotypes (D), intestinal microbiota (I), enzymes (E) and traits of metabolism (T) named as "DIET," which can provide a reference for further study of the mechanism in regulation of intestinal bacteria-mediated diet on diseases.
30564225	6	16	theme	diet	1581:1584	arg1	intake					1586:1591	non-isoflavones diet intake	1565:1591	non-isoflavones diet intake	1565:1591	Meanwhile, predicted metagenomics indicated that the pathway of glycan biosynthesis and metabolism was markedly down-regulated after non-isoflavones diet intake.
30564225	5	17	theme	Lactobacillus	1243:1255	arg1	β-glucosidase					1269:1281	Lactobacillus, produceing β-glucosidase	1243:1281	β-glucosidase	1269:1281	In addition, Lactobacillus, produceing β-glucosidase which contribute to biotransform glycosides into aglycons and exert the bioactivities consequently, was decreased after non-isoflavones diet intake.
30564225	1	18	theme	biochemical	312:322	arg1	state					324:328	biochemical state	312:328	biochemical state of rats	312:336	Isoflavones, presenting in leguminous plants and the normal chow diet, are known to alter intestinal microbiota, yet their deficiency has not been widely studied for its effect on constipation in biochemical state of rats.
30564225	3	19	theme	peptide	845:851	arg1	concentrations					784:797	the serum concentrations	774:797	the serum concentrations of substance P (SP) and vasoactive intestinal peptide (VIP)	774:857	To gain insight into the key role of intestinal microbiota in constipation and metabolic differences caused by isoflavones deficiency, we observed a significant decrease in fecal pellet numbers, fecal water content, intestinal transit rate together with the serum concentrations of substance P (SP) and vasoactive intestinal peptide (VIP) in NISO group, compared with those in the ISO group.
30564225	4	20	theme	16S	922:924	arg1	sequencing					945:954	16S rRNA compositional sequencing	922:954	16S rRNA compositional sequencing	922:954	Following 16S rRNA compositional sequencing, results excluded the changes in intestinal microbiota over time and highlighted that a total of 5 phyla and 21 genera changed significantly, among which Firmicutes, Bacteroidetes, Blautia, Prevotella, Lactobacillus and Bifidobacterium were closely related to constipation.
30564225	3	21	theme	substance	802:810	arg1	SP					815:816	SP	815:816	SP	815:816	To gain insight into the key role of intestinal microbiota in constipation and metabolic differences caused by isoflavones deficiency, we observed a significant decrease in fecal pellet numbers, fecal water content, intestinal transit rate together with the serum concentrations of substance P (SP) and vasoactive intestinal peptide (VIP) in NISO group, compared with those in the ISO group.
30564225	3	21	theme	substance	802:810	arg1	P					812:812	substance P	802:812	substance P (SP)	802:817	To gain insight into the key role of intestinal microbiota in constipation and metabolic differences caused by isoflavones deficiency, we observed a significant decrease in fecal pellet numbers, fecal water content, intestinal transit rate together with the serum concentrations of substance P (SP) and vasoactive intestinal peptide (VIP) in NISO group, compared with those in the ISO group.
30564225	1	22	theme	normal	169:174	arg1	diet					181:184	the normal chow diet	165:184	the normal chow diet	165:184	Isoflavones, presenting in leguminous plants and the normal chow diet, are known to alter intestinal microbiota, yet their deficiency has not been widely studied for its effect on constipation in biochemical state of rats.
30564225	3	23	theme	vasoactive	823:832	arg1	VIP					854:856	VIP	854:856	VIP	854:856	To gain insight into the key role of intestinal microbiota in constipation and metabolic differences caused by isoflavones deficiency, we observed a significant decrease in fecal pellet numbers, fecal water content, intestinal transit rate together with the serum concentrations of substance P (SP) and vasoactive intestinal peptide (VIP) in NISO group, compared with those in the ISO group.
30564225	3	23	theme	vasoactive	823:832	arg1	peptide					845:851	vasoactive intestinal peptide	823:851	vasoactive intestinal peptide (VIP)	823:857	To gain insight into the key role of intestinal microbiota in constipation and metabolic differences caused by isoflavones deficiency, we observed a significant decrease in fecal pellet numbers, fecal water content, intestinal transit rate together with the serum concentrations of substance P (SP) and vasoactive intestinal peptide (VIP) in NISO group, compared with those in the ISO group.
30564225	0	24	theme	Gut	100:102	arg1	Microbiota					104:113	Targeting Gut Microbiota	90:113	Targeting Gut Microbiota	90:113	Non-isoflavones Diet Incurred Metabolic Modifications Induced by Constipation in Rats via Targeting Gut Microbiota.
30564225	7	25	theme	biochemical	1692:1702	arg1	state					1704:1708	the biochemical state	1688:1708	the biochemical state of rats	1688:1716	Taken together, the findings suggested that the changes in the dietary components could alter the biochemical state of rats, which may be triggered by the abnormal modifications facilitated by β-glucosidase-producing bacteria.
30564225	1	26	theme	leguminous	143:152	arg1	plants					154:159	leguminous plants	143:159	leguminous plants	143:159	Isoflavones, presenting in leguminous plants and the normal chow diet, are known to alter intestinal microbiota, yet their deficiency has not been widely studied for its effect on constipation in biochemical state of rats.
30564225	2	27	theme	non-isoflavones	491:505	arg1	NISO					513:516	NISO	513:516	NISO	513:516	Our previous study discovered the differences in pharmacokinetic traits of isoflavones from Semen sojae praeparatum fed with normal chow diet (ISO) and non-isoflavones diet (NISO).
30564225	2	27	theme	non-isoflavones	491:505	arg1	diet					507:510	non-isoflavones diet	491:510	non-isoflavones diet (NISO)	491:517	Our previous study discovered the differences in pharmacokinetic traits of isoflavones from Semen sojae praeparatum fed with normal chow diet (ISO) and non-isoflavones diet (NISO).
30564225	8	28	theme	metabolism	1962:1971	arg1	traits					1952:1957	traits	1952:1957	traits of metabolism (T) named as "DIET," which can provide a reference for further study of the mechanism in regulation of intestinal bacteria-mediated diet on diseases	1952:2120	Our study shed a new strategy to explore the relationship among disease phenotypes (D), intestinal microbiota (I), enzymes (E) and traits of metabolism (T) named as "DIET," which can provide a reference for further study of the mechanism in regulation of intestinal bacteria-mediated diet on diseases.
30564225	8	28	theme	metabolism	1962:1971	arg1	enzymes					1936:1942	enzymes	1936:1942	enzymes (E)	1936:1946	Our study shed a new strategy to explore the relationship among disease phenotypes (D), intestinal microbiota (I), enzymes (E) and traits of metabolism (T) named as "DIET," which can provide a reference for further study of the mechanism in regulation of intestinal bacteria-mediated diet on diseases.
30564225	8	28	theme	metabolism	1962:1971	arg1	microbiota					1920:1929	intestinal microbiota	1909:1929	intestinal microbiota (I)	1909:1933	Our study shed a new strategy to explore the relationship among disease phenotypes (D), intestinal microbiota (I), enzymes (E) and traits of metabolism (T) named as "DIET," which can provide a reference for further study of the mechanism in regulation of intestinal bacteria-mediated diet on diseases.
30564225	8	28	theme	metabolism	1962:1971	arg1	E					1945:1945	E	1945:1945	E	1945:1945	Our study shed a new strategy to explore the relationship among disease phenotypes (D), intestinal microbiota (I), enzymes (E) and traits of metabolism (T) named as "DIET," which can provide a reference for further study of the mechanism in regulation of intestinal bacteria-mediated diet on diseases.
30564225	8	28	theme	metabolism	1962:1971	arg1	phenotypes					1893:1902	disease phenotypes	1885:1902	disease phenotypes (D)	1885:1906	Our study shed a new strategy to explore the relationship among disease phenotypes (D), intestinal microbiota (I), enzymes (E) and traits of metabolism (T) named as "DIET," which can provide a reference for further study of the mechanism in regulation of intestinal bacteria-mediated diet on diseases.
30564225	8	28	theme	metabolism	1962:1971	arg1	T					1974:1974	T	1974:1974	T	1974:1974	Our study shed a new strategy to explore the relationship among disease phenotypes (D), intestinal microbiota (I), enzymes (E) and traits of metabolism (T) named as "DIET," which can provide a reference for further study of the mechanism in regulation of intestinal bacteria-mediated diet on diseases.
30564225	8	28	theme	metabolism	1962:1971	arg1	D					1905:1905	D	1905:1905	D	1905:1905	Our study shed a new strategy to explore the relationship among disease phenotypes (D), intestinal microbiota (I), enzymes (E) and traits of metabolism (T) named as "DIET," which can provide a reference for further study of the mechanism in regulation of intestinal bacteria-mediated diet on diseases.
30564225	8	28	theme	metabolism	1962:1971	arg1	I					1932:1932	I	1932:1932	I	1932:1932	Our study shed a new strategy to explore the relationship among disease phenotypes (D), intestinal microbiota (I), enzymes (E) and traits of metabolism (T) named as "DIET," which can provide a reference for further study of the mechanism in regulation of intestinal bacteria-mediated diet on diseases.
30564225	2	29	theme	chow	471:474	arg1	ISO					482:484	ISO	482:484	ISO	482:484	Our previous study discovered the differences in pharmacokinetic traits of isoflavones from Semen sojae praeparatum fed with normal chow diet (ISO) and non-isoflavones diet (NISO).
30564225	2	29	theme	chow	471:474	arg1	diet					476:479	normal chow diet	464:479	normal chow diet (ISO)	464:485	Our previous study discovered the differences in pharmacokinetic traits of isoflavones from Semen sojae praeparatum fed with normal chow diet (ISO) and non-isoflavones diet (NISO).
30564225	0	30	theme	Non-isoflavones	0:14	arg1	Diet					16:19	Non-isoflavones Diet	0:19	Non-isoflavones Diet	0:19	Non-isoflavones Diet Incurred Metabolic Modifications Induced by Constipation in Rats via Targeting Gut Microbiota.
30564225	4	31	from	changes	978:984	arg1	microbiota					1000:1009	intestinal microbiota	989:1009	intestinal microbiota	989:1009	Following 16S rRNA compositional sequencing, results excluded the changes in intestinal microbiota over time and highlighted that a total of 5 phyla and 21 genera changed significantly, among which Firmicutes, Bacteroidetes, Blautia, Prevotella, Lactobacillus and Bifidobacterium were closely related to constipation.
30564225	2	32	theme	Semen	431:435	arg1	praeparatum					443:453	Semen sojae praeparatum	431:453	Semen sojae praeparatum fed with normal chow diet (ISO) and non-isoflavones diet (NISO)	431:517	Our previous study discovered the differences in pharmacokinetic traits of isoflavones from Semen sojae praeparatum fed with normal chow diet (ISO) and non-isoflavones diet (NISO).
30564225	3	33	from	role	549:552	arg1	constipation					582:593	constipation	582:593	constipation	582:593	To gain insight into the key role of intestinal microbiota in constipation and metabolic differences caused by isoflavones deficiency, we observed a significant decrease in fecal pellet numbers, fecal water content, intestinal transit rate together with the serum concentrations of substance P (SP) and vasoactive intestinal peptide (VIP) in NISO group, compared with those in the ISO group.
30564225	2	34	theme	isoflavones	414:424	arg1	traits					404:409	pharmacokinetic traits	388:409	pharmacokinetic traits of isoflavones from Semen sojae praeparatum fed with normal chow diet (ISO) and non-isoflavones diet (NISO)	388:517	Our previous study discovered the differences in pharmacokinetic traits of isoflavones from Semen sojae praeparatum fed with normal chow diet (ISO) and non-isoflavones diet (NISO).
30564225	4	35	theme	compositional	931:943	arg1	sequencing					945:954	16S rRNA compositional sequencing	922:954	16S rRNA compositional sequencing	922:954	Following 16S rRNA compositional sequencing, results excluded the changes in intestinal microbiota over time and highlighted that a total of 5 phyla and 21 genera changed significantly, among which Firmicutes, Bacteroidetes, Blautia, Prevotella, Lactobacillus and Bifidobacterium were closely related to constipation.
30564225	5	36	theme	produceing	1258:1267	arg1	β-glucosidase					1269:1281	Lactobacillus, produceing β-glucosidase	1243:1281	β-glucosidase	1269:1281	In addition, Lactobacillus, produceing β-glucosidase which contribute to biotransform glycosides into aglycons and exert the bioactivities consequently, was decreased after non-isoflavones diet intake.
30564225	4	37	theme	rRNA	926:929	arg1	sequencing					945:954	16S rRNA compositional sequencing	922:954	16S rRNA compositional sequencing	922:954	Following 16S rRNA compositional sequencing, results excluded the changes in intestinal microbiota over time and highlighted that a total of 5 phyla and 21 genera changed significantly, among which Firmicutes, Bacteroidetes, Blautia, Prevotella, Lactobacillus and Bifidobacterium were closely related to constipation.
30564225	8	38	theme	further	2028:2034	arg1	study					2036:2040	further study	2028:2040	further study of the mechanism in regulation of intestinal bacteria-mediated diet on diseases	2028:2120	Our study shed a new strategy to explore the relationship among disease phenotypes (D), intestinal microbiota (I), enzymes (E) and traits of metabolism (T) named as "DIET," which can provide a reference for further study of the mechanism in regulation of intestinal bacteria-mediated diet on diseases.
30564225	8	39	from	study	2036:2040	arg1	regulation					2062:2071	regulation	2062:2071	regulation of intestinal bacteria-mediated diet on diseases	2062:2120	Our study shed a new strategy to explore the relationship among disease phenotypes (D), intestinal microbiota (I), enzymes (E) and traits of metabolism (T) named as "DIET," which can provide a reference for further study of the mechanism in regulation of intestinal bacteria-mediated diet on diseases.
30564225	8	40	theme	mechanism	2049:2057	arg1	study					2036:2040	further study	2028:2040	further study of the mechanism in regulation of intestinal bacteria-mediated diet on diseases	2028:2120	Our study shed a new strategy to explore the relationship among disease phenotypes (D), intestinal microbiota (I), enzymes (E) and traits of metabolism (T) named as "DIET," which can provide a reference for further study of the mechanism in regulation of intestinal bacteria-mediated diet on diseases.
30564225	4	41	theme	intestinal	989:998	arg1	microbiota					1000:1009	intestinal microbiota	989:1009	intestinal microbiota	989:1009	Following 16S rRNA compositional sequencing, results excluded the changes in intestinal microbiota over time and highlighted that a total of 5 phyla and 21 genera changed significantly, among which Firmicutes, Bacteroidetes, Blautia, Prevotella, Lactobacillus and Bifidobacterium were closely related to constipation.
30564225	8	42	theme	intestinal	2076:2085	arg1	diet					2105:2108	intestinal bacteria-mediated diet	2076:2108	intestinal bacteria-mediated diet on diseases	2076:2120	Our study shed a new strategy to explore the relationship among disease phenotypes (D), intestinal microbiota (I), enzymes (E) and traits of metabolism (T) named as "DIET," which can provide a reference for further study of the mechanism in regulation of intestinal bacteria-mediated diet on diseases.
30564225	1	43	from	effect	286:291	arg1	constipation					296:307	constipation	296:307	constipation	296:307	Isoflavones, presenting in leguminous plants and the normal chow diet, are known to alter intestinal microbiota, yet their deficiency has not been widely studied for its effect on constipation in biochemical state of rats.
30564225	1	43	from	effect	286:291	arg1	state					324:328	biochemical state	312:328	biochemical state of rats	312:336	Isoflavones, presenting in leguminous plants and the normal chow diet, are known to alter intestinal microbiota, yet their deficiency has not been widely studied for its effect on constipation in biochemical state of rats.
30564225	5	44	theme	non-isoflavones	1403:1417	arg1	intake					1424:1429	non-isoflavones diet intake	1403:1429	non-isoflavones diet intake	1403:1429	In addition, Lactobacillus, produceing β-glucosidase which contribute to biotransform glycosides into aglycons and exert the bioactivities consequently, was decreased after non-isoflavones diet intake.
30564225	8	45	theme	diet	2105:2108	arg1	regulation					2062:2071	regulation	2062:2071	regulation of intestinal bacteria-mediated diet on diseases	2062:2120	Our study shed a new strategy to explore the relationship among disease phenotypes (D), intestinal microbiota (I), enzymes (E) and traits of metabolism (T) named as "DIET," which can provide a reference for further study of the mechanism in regulation of intestinal bacteria-mediated diet on diseases.
30564225	1	46	theme	chow	176:179	arg1	diet					181:184	the normal chow diet	165:184	the normal chow diet	165:184	Isoflavones, presenting in leguminous plants and the normal chow diet, are known to alter intestinal microbiota, yet their deficiency has not been widely studied for its effect on constipation in biochemical state of rats.
30564225	4	47	theme	genera	1068:1073	arg1	total					1044:1048	a total	1042:1048	a total of 5 phyla and 21 genera	1042:1073	Following 16S rRNA compositional sequencing, results excluded the changes in intestinal microbiota over time and highlighted that a total of 5 phyla and 21 genera changed significantly, among which Firmicutes, Bacteroidetes, Blautia, Prevotella, Lactobacillus and Bifidobacterium were closely related to constipation.
30564225	3	48	theme	fecal	693:697	arg1	numbers					706:712	fecal pellet numbers	693:712	fecal pellet numbers	693:712	To gain insight into the key role of intestinal microbiota in constipation and metabolic differences caused by isoflavones deficiency, we observed a significant decrease in fecal pellet numbers, fecal water content, intestinal transit rate together with the serum concentrations of substance P (SP) and vasoactive intestinal peptide (VIP) in NISO group, compared with those in the ISO group.
30564225	8	49	theme	intestinal	1909:1918	arg1	microbiota					1920:1929	intestinal microbiota	1909:1929	intestinal microbiota (I)	1909:1933	Our study shed a new strategy to explore the relationship among disease phenotypes (D), intestinal microbiota (I), enzymes (E) and traits of metabolism (T) named as "DIET," which can provide a reference for further study of the mechanism in regulation of intestinal bacteria-mediated diet on diseases.
30564225	8	49	theme	intestinal	1909:1918	arg1	I					1932:1932	I	1932:1932	I	1932:1932	Our study shed a new strategy to explore the relationship among disease phenotypes (D), intestinal microbiota (I), enzymes (E) and traits of metabolism (T) named as "DIET," which can provide a reference for further study of the mechanism in regulation of intestinal bacteria-mediated diet on diseases.
30564225	6	50	theme	glycan	1496:1501	arg1	biosynthesis					1503:1514	glycan biosynthesis	1496:1514	glycan biosynthesis	1496:1514	Meanwhile, predicted metagenomics indicated that the pathway of glycan biosynthesis and metabolism was markedly down-regulated after non-isoflavones diet intake.
30564225	3	51	theme	fecal	715:719	arg1	content					727:733	fecal water content	715:733	fecal water content	715:733	To gain insight into the key role of intestinal microbiota in constipation and metabolic differences caused by isoflavones deficiency, we observed a significant decrease in fecal pellet numbers, fecal water content, intestinal transit rate together with the serum concentrations of substance P (SP) and vasoactive intestinal peptide (VIP) in NISO group, compared with those in the ISO group.
30564225	3	51	theme	fecal	715:719	arg1	decrease					681:688	a significant decrease	667:688	a significant decrease in fecal pellet numbers	667:712	To gain insight into the key role of intestinal microbiota in constipation and metabolic differences caused by isoflavones deficiency, we observed a significant decrease in fecal pellet numbers, fecal water content, intestinal transit rate together with the serum concentrations of substance P (SP) and vasoactive intestinal peptide (VIP) in NISO group, compared with those in the ISO group.
30564225	7	52	dep	alter	1682:1686	arg1	triggered					1732:1740	triggered	1732:1740	may be triggered by the abnormal modifications facilitated by β-glucosidase-producing bacteria	1725:1818	Taken together, the findings suggested that the changes in the dietary components could alter the biochemical state of rats, which may be triggered by the abnormal modifications facilitated by β-glucosidase-producing bacteria.
30564225	3	53	theme	key	545:547	arg1	role					549:552	the key role	541:552	the key role of intestinal microbiota in constipation	541:593	To gain insight into the key role of intestinal microbiota in constipation and metabolic differences caused by isoflavones deficiency, we observed a significant decrease in fecal pellet numbers, fecal water content, intestinal transit rate together with the serum concentrations of substance P (SP) and vasoactive intestinal peptide (VIP) in NISO group, compared with those in the ISO group.
30564225	8	54	from	regulation	2062:2071	arg1	diseases					2113:2120	diseases	2113:2120	diseases	2113:2120	Our study shed a new strategy to explore the relationship among disease phenotypes (D), intestinal microbiota (I), enzymes (E) and traits of metabolism (T) named as "DIET," which can provide a reference for further study of the mechanism in regulation of intestinal bacteria-mediated diet on diseases.
30564225	8	54	from	regulation	2062:2071	arg1	study					2036:2040	further study	2028:2040	further study of the mechanism in regulation of intestinal bacteria-mediated diet on diseases	2028:2120	Our study shed a new strategy to explore the relationship among disease phenotypes (D), intestinal microbiota (I), enzymes (E) and traits of metabolism (T) named as "DIET," which can provide a reference for further study of the mechanism in regulation of intestinal bacteria-mediated diet on diseases.
30564225	8	55	from	diseases	2113:2120	arg1	regulation					2062:2071	regulation	2062:2071	regulation of intestinal bacteria-mediated diet on diseases	2062:2120	Our study shed a new strategy to explore the relationship among disease phenotypes (D), intestinal microbiota (I), enzymes (E) and traits of metabolism (T) named as "DIET," which can provide a reference for further study of the mechanism in regulation of intestinal bacteria-mediated diet on diseases.
30564225	3	56	theme	serum	778:782	arg1	concentrations					784:797	the serum concentrations	774:797	the serum concentrations of substance P (SP) and vasoactive intestinal peptide (VIP)	774:857	To gain insight into the key role of intestinal microbiota in constipation and metabolic differences caused by isoflavones deficiency, we observed a significant decrease in fecal pellet numbers, fecal water content, intestinal transit rate together with the serum concentrations of substance P (SP) and vasoactive intestinal peptide (VIP) in NISO group, compared with those in the ISO group.
30564225	7	57	theme	rats	1713:1716	arg1	state					1704:1708	the biochemical state	1688:1708	the biochemical state of rats	1688:1716	Taken together, the findings suggested that the changes in the dietary components could alter the biochemical state of rats, which may be triggered by the abnormal modifications facilitated by β-glucosidase-producing bacteria.
30564225	3	58	theme	microbiota	568:577	arg1	role					549:552	the key role	541:552	the key role of intestinal microbiota in constipation	541:593	To gain insight into the key role of intestinal microbiota in constipation and metabolic differences caused by isoflavones deficiency, we observed a significant decrease in fecal pellet numbers, fecal water content, intestinal transit rate together with the serum concentrations of substance P (SP) and vasoactive intestinal peptide (VIP) in NISO group, compared with those in the ISO group.
30564225	3	58	theme	microbiota	568:577	arg1	differences					609:619	metabolic differences	599:619	metabolic differences caused by isoflavones deficiency	599:652	To gain insight into the key role of intestinal microbiota in constipation and metabolic differences caused by isoflavones deficiency, we observed a significant decrease in fecal pellet numbers, fecal water content, intestinal transit rate together with the serum concentrations of substance P (SP) and vasoactive intestinal peptide (VIP) in NISO group, compared with those in the ISO group.
30564225	6	59	theme	non-isoflavones	1565:1579	arg1	intake					1586:1591	non-isoflavones diet intake	1565:1591	non-isoflavones diet intake	1565:1591	Meanwhile, predicted metagenomics indicated that the pathway of glycan biosynthesis and metabolism was markedly down-regulated after non-isoflavones diet intake.
30564225	8	60	from	diet	2105:2108	arg1	diseases					2113:2120	diseases	2113:2120	diseases	2113:2120	Our study shed a new strategy to explore the relationship among disease phenotypes (D), intestinal microbiota (I), enzymes (E) and traits of metabolism (T) named as "DIET," which can provide a reference for further study of the mechanism in regulation of intestinal bacteria-mediated diet on diseases.
30564225	0	61	theme	Targeting	90:98	arg1	Microbiota					104:113	Targeting Gut Microbiota	90:113	Targeting Gut Microbiota	90:113	Non-isoflavones Diet Incurred Metabolic Modifications Induced by Constipation in Rats via Targeting Gut Microbiota.
30564225	2	62	from	praeparatum	443:453	arg1	traits					404:409	pharmacokinetic traits	388:409	pharmacokinetic traits of isoflavones from Semen sojae praeparatum fed with normal chow diet (ISO) and non-isoflavones diet (NISO)	388:517	Our previous study discovered the differences in pharmacokinetic traits of isoflavones from Semen sojae praeparatum fed with normal chow diet (ISO) and non-isoflavones diet (NISO).
30564225	2	62	from	praeparatum	443:453	arg1	isoflavones					414:424	isoflavones	414:424	isoflavones from Semen sojae praeparatum fed with normal chow diet (ISO) and non-isoflavones diet (NISO)	414:517	Our previous study discovered the differences in pharmacokinetic traits of isoflavones from Semen sojae praeparatum fed with normal chow diet (ISO) and non-isoflavones diet (NISO).
30564225	3	63	theme	P	812:812	arg1	concentrations					784:797	the serum concentrations	774:797	the serum concentrations of substance P (SP) and vasoactive intestinal peptide (VIP)	774:857	To gain insight into the key role of intestinal microbiota in constipation and metabolic differences caused by isoflavones deficiency, we observed a significant decrease in fecal pellet numbers, fecal water content, intestinal transit rate together with the serum concentrations of substance P (SP) and vasoactive intestinal peptide (VIP) in NISO group, compared with those in the ISO group.
30564225	1	64	theme	rats	333:336	arg1	state					324:328	biochemical state	312:328	biochemical state of rats	312:336	Isoflavones, presenting in leguminous plants and the normal chow diet, are known to alter intestinal microbiota, yet their deficiency has not been widely studied for its effect on constipation in biochemical state of rats.
30564225	3	65	theme	intestinal	834:843	arg1	VIP					854:856	VIP	854:856	VIP	854:856	To gain insight into the key role of intestinal microbiota in constipation and metabolic differences caused by isoflavones deficiency, we observed a significant decrease in fecal pellet numbers, fecal water content, intestinal transit rate together with the serum concentrations of substance P (SP) and vasoactive intestinal peptide (VIP) in NISO group, compared with those in the ISO group.
30564225	3	65	theme	intestinal	834:843	arg1	peptide					845:851	vasoactive intestinal peptide	823:851	vasoactive intestinal peptide (VIP)	823:857	To gain insight into the key role of intestinal microbiota in constipation and metabolic differences caused by isoflavones deficiency, we observed a significant decrease in fecal pellet numbers, fecal water content, intestinal transit rate together with the serum concentrations of substance P (SP) and vasoactive intestinal peptide (VIP) in NISO group, compared with those in the ISO group.
30564225	2	66	from	traits	404:409	arg1	praeparatum					443:453	Semen sojae praeparatum	431:453	Semen sojae praeparatum fed with normal chow diet (ISO) and non-isoflavones diet (NISO)	431:517	Our previous study discovered the differences in pharmacokinetic traits of isoflavones from Semen sojae praeparatum fed with normal chow diet (ISO) and non-isoflavones diet (NISO).
30564225	3	67	theme	NISO	862:865	arg1	group					867:871	NISO group	862:871	NISO group	862:871	To gain insight into the key role of intestinal microbiota in constipation and metabolic differences caused by isoflavones deficiency, we observed a significant decrease in fecal pellet numbers, fecal water content, intestinal transit rate together with the serum concentrations of substance P (SP) and vasoactive intestinal peptide (VIP) in NISO group, compared with those in the ISO group.
30564225	7	68	from	changes	1642:1648	arg1	components					1665:1674	the dietary components	1653:1674	the dietary components	1653:1674	Taken together, the findings suggested that the changes in the dietary components could alter the biochemical state of rats, which may be triggered by the abnormal modifications facilitated by β-glucosidase-producing bacteria.
30564225	8	69	theme	new	1838:1840	arg1	strategy					1842:1849	a new strategy	1836:1849	a new strategy to explore the relationship among disease phenotypes (D), intestinal microbiota (I), enzymes (E) and traits of metabolism (T) named as "DIET," which can provide a reference for further study of the mechanism in regulation of intestinal bacteria-mediated diet on diseases	1836:2120	Our study shed a new strategy to explore the relationship among disease phenotypes (D), intestinal microbiota (I), enzymes (E) and traits of metabolism (T) named as "DIET," which can provide a reference for further study of the mechanism in regulation of intestinal bacteria-mediated diet on diseases.
30564225	2	70	theme	sojae	437:441	arg1	praeparatum					443:453	Semen sojae praeparatum	431:453	Semen sojae praeparatum fed with normal chow diet (ISO) and non-isoflavones diet (NISO)	431:517	Our previous study discovered the differences in pharmacokinetic traits of isoflavones from Semen sojae praeparatum fed with normal chow diet (ISO) and non-isoflavones diet (NISO).
30564225	0	71	theme	Metabolic	30:38	arg1	Modifications					40:52	Metabolic Modifications	30:52	Metabolic Modifications Induced by Constipation in Rats	30:84	Non-isoflavones Diet Incurred Metabolic Modifications Induced by Constipation in Rats via Targeting Gut Microbiota.
30564225	8	72	theme	DIET	1987:1990	arg1	"					1992:1992	DIET,"	1987:1992	"	1992:1992	Our study shed a new strategy to explore the relationship among disease phenotypes (D), intestinal microbiota (I), enzymes (E) and traits of metabolism (T) named as "DIET," which can provide a reference for further study of the mechanism in regulation of intestinal bacteria-mediated diet on diseases.
30564225	7	73	theme	dietary	1657:1663	arg1	components					1665:1674	the dietary components	1653:1674	the dietary components	1653:1674	Taken together, the findings suggested that the changes in the dietary components could alter the biochemical state of rats, which may be triggered by the abnormal modifications facilitated by β-glucosidase-producing bacteria.
30564225	2	74	theme	normal	464:469	arg1	ISO					482:484	ISO	482:484	ISO	482:484	Our previous study discovered the differences in pharmacokinetic traits of isoflavones from Semen sojae praeparatum fed with normal chow diet (ISO) and non-isoflavones diet (NISO).
30564225	2	74	theme	normal	464:469	arg1	diet					476:479	normal chow diet	464:479	normal chow diet (ISO)	464:485	Our previous study discovered the differences in pharmacokinetic traits of isoflavones from Semen sojae praeparatum fed with normal chow diet (ISO) and non-isoflavones diet (NISO).
30564225	3	75	theme	metabolic	599:607	arg1	differences					609:619	metabolic differences	599:619	metabolic differences caused by isoflavones deficiency	599:652	To gain insight into the key role of intestinal microbiota in constipation and metabolic differences caused by isoflavones deficiency, we observed a significant decrease in fecal pellet numbers, fecal water content, intestinal transit rate together with the serum concentrations of substance P (SP) and vasoactive intestinal peptide (VIP) in NISO group, compared with those in the ISO group.
30564225	2	76	theme	pharmacokinetic	388:402	arg1	traits					404:409	pharmacokinetic traits	388:409	pharmacokinetic traits of isoflavones from Semen sojae praeparatum fed with normal chow diet (ISO) and non-isoflavones diet (NISO)	388:517	Our previous study discovered the differences in pharmacokinetic traits of isoflavones from Semen sojae praeparatum fed with normal chow diet (ISO) and non-isoflavones diet (NISO).
30564225	3	77	theme	ISO	901:903	arg1	group					905:909	the ISO group	897:909	the ISO group	897:909	To gain insight into the key role of intestinal microbiota in constipation and metabolic differences caused by isoflavones deficiency, we observed a significant decrease in fecal pellet numbers, fecal water content, intestinal transit rate together with the serum concentrations of substance P (SP) and vasoactive intestinal peptide (VIP) in NISO group, compared with those in the ISO group.
30564225	4	78	dep	Prevotella	1146:1155	arg1	Blautia					1137:1143	Blautia	1137:1143	Blautia	1137:1143	Following 16S rRNA compositional sequencing, results excluded the changes in intestinal microbiota over time and highlighted that a total of 5 phyla and 21 genera changed significantly, among which Firmicutes, Bacteroidetes, Blautia, Prevotella, Lactobacillus and Bifidobacterium were closely related to constipation.
30564225	2	79	from	differences	373:383	arg1	traits					404:409	pharmacokinetic traits	388:409	pharmacokinetic traits of isoflavones from Semen sojae praeparatum fed with normal chow diet (ISO) and non-isoflavones diet (NISO)	388:517	Our previous study discovered the differences in pharmacokinetic traits of isoflavones from Semen sojae praeparatum fed with normal chow diet (ISO) and non-isoflavones diet (NISO).
30564225	3	80	theme	isoflavones	631:641	arg1	deficiency					643:652	isoflavones deficiency	631:652	isoflavones deficiency	631:652	To gain insight into the key role of intestinal microbiota in constipation and metabolic differences caused by isoflavones deficiency, we observed a significant decrease in fecal pellet numbers, fecal water content, intestinal transit rate together with the serum concentrations of substance P (SP) and vasoactive intestinal peptide (VIP) in NISO group, compared with those in the ISO group.
30564225	5	81	theme	biotransform	1303:1314	arg1	glycosides					1316:1325	biotransform glycosides	1303:1325	biotransform glycosides into aglycons	1303:1339	In addition, Lactobacillus, produceing β-glucosidase which contribute to biotransform glycosides into aglycons and exert the bioactivities consequently, was decreased after non-isoflavones diet intake.
30564225	1	82	theme	intestinal	206:215	arg1	microbiota					217:226	intestinal microbiota	206:226	intestinal microbiota	206:226	Isoflavones, presenting in leguminous plants and the normal chow diet, are known to alter intestinal microbiota, yet their deficiency has not been widely studied for its effect on constipation in biochemical state of rats.
30564225	3	83	theme	significant	669:679	arg1	decrease					681:688	a significant decrease	667:688	a significant decrease in fecal pellet numbers	667:712	To gain insight into the key role of intestinal microbiota in constipation and metabolic differences caused by isoflavones deficiency, we observed a significant decrease in fecal pellet numbers, fecal water content, intestinal transit rate together with the serum concentrations of substance P (SP) and vasoactive intestinal peptide (VIP) in NISO group, compared with those in the ISO group.
30564225	3	83	theme	significant	669:679	arg1	content					727:733	fecal water content	715:733	fecal water content	715:733	To gain insight into the key role of intestinal microbiota in constipation and metabolic differences caused by isoflavones deficiency, we observed a significant decrease in fecal pellet numbers, fecal water content, intestinal transit rate together with the serum concentrations of substance P (SP) and vasoactive intestinal peptide (VIP) in NISO group, compared with those in the ISO group.
30564225	3	83	theme	significant	669:679	arg1	rate					755:758	intestinal transit rate	736:758	intestinal transit rate	736:758	To gain insight into the key role of intestinal microbiota in constipation and metabolic differences caused by isoflavones deficiency, we observed a significant decrease in fecal pellet numbers, fecal water content, intestinal transit rate together with the serum concentrations of substance P (SP) and vasoactive intestinal peptide (VIP) in NISO group, compared with those in the ISO group.
30564225	2	84	theme	previous	343:350	arg1	study					352:356	Our previous study	339:356	Our previous study	339:356	Our previous study discovered the differences in pharmacokinetic traits of isoflavones from Semen sojae praeparatum fed with normal chow diet (ISO) and non-isoflavones diet (NISO).
30564225	6	85	theme	predicted	1443:1451	arg1	Meanwhile					1432:1440	Meanwhile	1432:1440	Meanwhile	1432:1440	Meanwhile, predicted metagenomics indicated that the pathway of glycan biosynthesis and metabolism was markedly down-regulated after non-isoflavones diet intake.
30564225	6	85	theme	predicted	1443:1451	arg1	metagenomics					1453:1464	predicted metagenomics	1443:1464	predicted metagenomics	1443:1464	Meanwhile, predicted metagenomics indicated that the pathway of glycan biosynthesis and metabolism was markedly down-regulated after non-isoflavones diet intake.
31670365	2	0	theme	peptide	540:546	arg1	sequence					548:555	peptide sequence	540:555	peptide sequence	540:555	Amongst such ligands, carbohydrate-binding peptides (CBPs) are attractive for use in bioanalytical and biomedical systems due to their biochemical and physicochemical properties; moreover, given the biological significance of lectin-carbohydrate interactions, these ligands offer an opportunity to study peptide sequence and binding characteristics to inform on natural target/ligand interactions.
31670365	2	1	theme	physicochemical	387:401	arg1	properties					403:412	their biochemical and physicochemical properties	365:412	their biochemical and physicochemical properties	365:412	Amongst such ligands, carbohydrate-binding peptides (CBPs) are attractive for use in bioanalytical and biomedical systems due to their biochemical and physicochemical properties; moreover, given the biological significance of lectin-carbohydrate interactions, these ligands offer an opportunity to study peptide sequence and binding characteristics to inform on natural target/ligand interactions.
31670365	5	2	from	screening	1038:1046	arg1	peptides					1010:1017	PSA-binding peptides	998:1017	PSA-binding peptides from phage display screening and from epitope mapping of an scFv for oligosialic acid	998:1103	PSA-binding peptides from phage display screening and from epitope mapping of an scFv for oligosialic acid were screened in an optimized microarray format with three ligand density conditions.
31670365	9	3	theme	peptide	1794:1800	arg1	characterization					1802:1817	peptide characterization	1794:1817	peptide characterization	1794:1817	Thus, the integrated approach described, with attention to design strategy, screening, and peptide characterization, successfully identified novel PSA-binding ligands and offers a platform for the identification and study of additional polysaccharide-binding peptides.
31670365	9	4	theme	integrated	1713:1722	arg1	approach					1724:1731	the integrated approach	1709:1731	the integrated approach described	1709:1741	Thus, the integrated approach described, with attention to design strategy, screening, and peptide characterization, successfully identified novel PSA-binding ligands and offers a platform for the identification and study of additional polysaccharide-binding peptides.
31670365	5	5	theme	oligosialic	1088:1098	arg1	acid					1100:1103	oligosialic acid	1088:1103	oligosialic acid	1088:1103	PSA-binding peptides from phage display screening and from epitope mapping of an scFv for oligosialic acid were screened in an optimized microarray format with three ligand density conditions.
31670365	8	6	theme	selectivity	1517:1527	arg1	studies					1529:1535	selectivity studies	1517:1535	selectivity studies performed directly on microarrays with chondroitin sulfate A (CS-A)	1517:1603	Furthermore, selectivity studies performed directly on microarrays with chondroitin sulfate A (CS-A) demonstrated the value of screening for both affinity and selectivity in the development of CBPs.
31670365	5	7	theme	scFv	1079:1082	arg1	mapping					1065:1071	epitope mapping	1057:1071	epitope mapping of an scFv for oligosialic acid	1057:1103	PSA-binding peptides from phage display screening and from epitope mapping of an scFv for oligosialic acid were screened in an optimized microarray format with three ligand density conditions.
31670365	4	8	theme	applications	984:995	arg1	number					947:952	a number	945:952	a number of diagnostic and therapeutic applications	945:995	The chemical and biological uniqueness of PSA suggests that an ability to exclusively target this glycan may promote a number of diagnostic and therapeutic applications.
31670365	8	9	theme	screening	1631:1639	arg1	value					1622:1626	the value	1618:1626	the value of screening for both affinity and selectivity	1618:1673	Furthermore, selectivity studies performed directly on microarrays with chondroitin sulfate A (CS-A) demonstrated the value of screening for both affinity and selectivity in the development of CBPs.
31670365	9	10	theme	design	1762:1767	arg1	strategy					1769:1776	design strategy	1762:1776	design strategy	1762:1776	Thus, the integrated approach described, with attention to design strategy, screening, and peptide characterization, successfully identified novel PSA-binding ligands and offers a platform for the identification and study of additional polysaccharide-binding peptides.
31670365	5	11	theme	phage	1024:1028	arg1	screening					1038:1046	phage display screening	1024:1046	phage display screening	1024:1046	PSA-binding peptides from phage display screening and from epitope mapping of an scFv for oligosialic acid were screened in an optimized microarray format with three ligand density conditions.
31670365	6	12	theme	Hypothesis-driven	1191:1207	arg1	mutations					1209:1217	Hypothesis-driven mutations	1191:1217	Hypothesis-driven mutations	1191:1217	Hypothesis-driven mutations were additionally applied to select peptides to modulate peptide affinity and selectivity to PSA.
31670365	1	13	dep	crucial	134:140	arg1	expanding					146:154	expanding	146:154	expanding knowledge on the glycocode	146:181	The development of carbohydrate-binding ligands is crucial for expanding knowledge on the glycocode and for achieving systematic carbohydrate targeting.
31670365	1	13	dep	crucial	134:140	arg1	crucial					134:140	crucial	134:140	crucial	134:140	The development of carbohydrate-binding ligands is crucial for expanding knowledge on the glycocode and for achieving systematic carbohydrate targeting.
31670365	1	13	dep	crucial	134:140	arg1	development					87:97	The development	83:97	The development of carbohydrate-binding ligands	83:129	The development of carbohydrate-binding ligands is crucial for expanding knowledge on the glycocode and for achieving systematic carbohydrate targeting.
31670365	1	13	dep	crucial	134:140	arg1	achieving					191:199	achieving	191:199	achieving systematic carbohydrate targeting	191:233	The development of carbohydrate-binding ligands is crucial for expanding knowledge on the glycocode and for achieving systematic carbohydrate targeting.
31670365	5	14	from	mapping	1065:1071	arg1	peptides					1010:1017	PSA-binding peptides	998:1017	PSA-binding peptides from phage display screening and from epitope mapping of an scFv for oligosialic acid	998:1103	PSA-binding peptides from phage display screening and from epitope mapping of an scFv for oligosialic acid were screened in an optimized microarray format with three ligand density conditions.
31670365	2	15	theme	binding	561:567	arg1	characteristics					569:583	binding characteristics	561:583	binding characteristics	561:583	Amongst such ligands, carbohydrate-binding peptides (CBPs) are attractive for use in bioanalytical and biomedical systems due to their biochemical and physicochemical properties; moreover, given the biological significance of lectin-carbohydrate interactions, these ligands offer an opportunity to study peptide sequence and binding characteristics to inform on natural target/ligand interactions.
31670365	5	16	theme	density	1171:1177	arg1	conditions					1179:1188	three ligand density conditions	1158:1188	three ligand density conditions	1158:1188	PSA-binding peptides from phage display screening and from epitope mapping of an scFv for oligosialic acid were screened in an optimized microarray format with three ligand density conditions.
31670365	8	17	theme	sulfate	1588:1594	arg1	A					1596:1596	chondroitin sulfate A	1576:1596	chondroitin sulfate A (CS-A)	1576:1603	Furthermore, selectivity studies performed directly on microarrays with chondroitin sulfate A (CS-A) demonstrated the value of screening for both affinity and selectivity in the development of CBPs.
31670365	8	17	theme	sulfate	1588:1594	arg1	CS-A					1599:1602	CS-A	1599:1602	CS-A	1599:1602	Furthermore, selectivity studies performed directly on microarrays with chondroitin sulfate A (CS-A) demonstrated the value of screening for both affinity and selectivity in the development of CBPs.
31670365	8	18	theme	CBPs	1697:1700	arg1	development					1682:1692	the development	1678:1692	the development of CBPs	1678:1700	Furthermore, selectivity studies performed directly on microarrays with chondroitin sulfate A (CS-A) demonstrated the value of screening for both affinity and selectivity in the development of CBPs.
31670365	5	19	theme	ligand	1164:1169	arg1	conditions					1179:1188	three ligand density conditions	1158:1188	three ligand density conditions	1158:1188	PSA-binding peptides from phage display screening and from epitope mapping of an scFv for oligosialic acid were screened in an optimized microarray format with three ligand density conditions.
31670365	1	20	from	knowledge	156:164	arg1	glycocode					173:181	the glycocode	169:181	the glycocode	169:181	The development of carbohydrate-binding ligands is crucial for expanding knowledge on the glycocode and for achieving systematic carbohydrate targeting.
31670365	9	21	dep	identification	1900:1913	arg1	the					1896:1898	the	1896:1898	the	1896:1898	Thus, the integrated approach described, with attention to design strategy, screening, and peptide characterization, successfully identified novel PSA-binding ligands and offers a platform for the identification and study of additional polysaccharide-binding peptides.
31670365	1	22	theme	systematic	201:210	arg1	targeting					225:233	systematic carbohydrate targeting	201:233	systematic carbohydrate targeting	201:233	The development of carbohydrate-binding ligands is crucial for expanding knowledge on the glycocode and for achieving systematic carbohydrate targeting.
31670365	4	23	theme	therapeutic	972:982	arg1	applications					984:995	diagnostic and therapeutic applications	957:995	diagnostic and therapeutic applications	957:995	The chemical and biological uniqueness of PSA suggests that an ability to exclusively target this glycan may promote a number of diagnostic and therapeutic applications.
31670365	6	24	theme	peptide	1276:1282	arg1	affinity					1284:1291	peptide affinity	1276:1291	peptide affinity	1276:1291	Hypothesis-driven mutations were additionally applied to select peptides to modulate peptide affinity and selectivity to PSA.
31670365	2	25	theme	biomedical	339:348	arg1	systems					350:356	bioanalytical and biomedical systems	321:356	bioanalytical and biomedical systems due to their biochemical and physicochemical properties	321:412	Amongst such ligands, carbohydrate-binding peptides (CBPs) are attractive for use in bioanalytical and biomedical systems due to their biochemical and physicochemical properties; moreover, given the biological significance of lectin-carbohydrate interactions, these ligands offer an opportunity to study peptide sequence and binding characteristics to inform on natural target/ligand interactions.
31670365	9	26	theme	polysaccharide-binding	1939:1960	arg1	peptides					1962:1969	additional polysaccharide-binding peptides	1928:1969	additional polysaccharide-binding peptides	1928:1969	Thus, the integrated approach described, with attention to design strategy, screening, and peptide characterization, successfully identified novel PSA-binding ligands and offers a platform for the identification and study of additional polysaccharide-binding peptides.
31670365	1	27	theme	carbohydrate	212:223	arg1	targeting					225:233	systematic carbohydrate targeting	201:233	systematic carbohydrate targeting	201:233	The development of carbohydrate-binding ligands is crucial for expanding knowledge on the glycocode and for achieving systematic carbohydrate targeting.
31670365	7	28	theme	residue	1463:1469	arg1	positioning					1471:1481	basic residue positioning	1457:1481	basic residue positioning	1457:1481	Peptide compositional and positional analyses revealed the significance of various residues for PSA binding and suggested the importance of basic residue positioning for PSA recognition.
31670365	5	29	theme	epitope	1057:1063	arg1	mapping					1065:1071	epitope mapping	1057:1071	epitope mapping of an scFv for oligosialic acid	1057:1103	PSA-binding peptides from phage display screening and from epitope mapping of an scFv for oligosialic acid were screened in an optimized microarray format with three ligand density conditions.
31670365	4	30	theme	diagnostic	957:966	arg1	applications					984:995	diagnostic and therapeutic applications	957:995	diagnostic and therapeutic applications	957:995	The chemical and biological uniqueness of PSA suggests that an ability to exclusively target this glycan may promote a number of diagnostic and therapeutic applications.
31670365	4	31	theme	PSA	870:872	arg1	uniqueness					856:865	The chemical and biological uniqueness	828:865	The chemical and biological uniqueness of PSA	828:872	The chemical and biological uniqueness of PSA suggests that an ability to exclusively target this glycan may promote a number of diagnostic and therapeutic applications.
31670365	1	32	theme	ligands	123:129	arg1	expanding					146:154	expanding	146:154	expanding knowledge on the glycocode	146:181	The development of carbohydrate-binding ligands is crucial for expanding knowledge on the glycocode and for achieving systematic carbohydrate targeting.
31670365	1	32	theme	ligands	123:129	arg1	crucial					134:140	crucial	134:140	crucial	134:140	The development of carbohydrate-binding ligands is crucial for expanding knowledge on the glycocode and for achieving systematic carbohydrate targeting.
31670365	1	32	theme	ligands	123:129	arg1	development					87:97	The development	83:97	The development of carbohydrate-binding ligands	83:129	The development of carbohydrate-binding ligands is crucial for expanding knowledge on the glycocode and for achieving systematic carbohydrate targeting.
31670365	1	32	theme	ligands	123:129	arg1	achieving					191:199	achieving	191:199	achieving systematic carbohydrate targeting	191:233	The development of carbohydrate-binding ligands is crucial for expanding knowledge on the glycocode and for achieving systematic carbohydrate targeting.
31670365	2	33	theme	lectin-carbohydrate	462:480	arg1	interactions					482:493	lectin-carbohydrate interactions	462:493	lectin-carbohydrate interactions	462:493	Amongst such ligands, carbohydrate-binding peptides (CBPs) are attractive for use in bioanalytical and biomedical systems due to their biochemical and physicochemical properties; moreover, given the biological significance of lectin-carbohydrate interactions, these ligands offer an opportunity to study peptide sequence and binding characteristics to inform on natural target/ligand interactions.
31670365	1	34	theme	carbohydrate-binding	102:121	arg1	ligands					123:129	carbohydrate-binding ligands	102:129	carbohydrate-binding ligands	102:129	The development of carbohydrate-binding ligands is crucial for expanding knowledge on the glycocode and for achieving systematic carbohydrate targeting.
31670365	2	35	theme	target/ligand	606:618	arg1	interactions					620:631	natural target/ligand interactions	598:631	natural target/ligand interactions	598:631	Amongst such ligands, carbohydrate-binding peptides (CBPs) are attractive for use in bioanalytical and biomedical systems due to their biochemical and physicochemical properties; moreover, given the biological significance of lectin-carbohydrate interactions, these ligands offer an opportunity to study peptide sequence and binding characteristics to inform on natural target/ligand interactions.
31670365	2	36	theme	bioanalytical	321:333	arg1	systems					350:356	bioanalytical and biomedical systems	321:356	bioanalytical and biomedical systems due to their biochemical and physicochemical properties	321:412	Amongst such ligands, carbohydrate-binding peptides (CBPs) are attractive for use in bioanalytical and biomedical systems due to their biochemical and physicochemical properties; moreover, given the biological significance of lectin-carbohydrate interactions, these ligands offer an opportunity to study peptide sequence and binding characteristics to inform on natural target/ligand interactions.
31670365	3	37	from	focus	751:755	arg1	PSA					777:779	PSA	777:779	PSA	777:779	Here, a high-throughput microarray screening technique is described for the identification and study of CBPs, with a focus on polysialic acid (PSA), a polysaccharide found on neural stem cells.
31670365	3	37	from	focus	751:755	arg1	acid					771:774	polysialic acid	760:774	polysialic acid (PSA)	760:780	Here, a high-throughput microarray screening technique is described for the identification and study of CBPs, with a focus on polysialic acid (PSA), a polysaccharide found on neural stem cells.
31670365	3	37	from	focus	751:755	arg1	polysaccharide					785:798	a polysaccharide	783:798	a polysaccharide found on neural stem cells	783:825	Here, a high-throughput microarray screening technique is described for the identification and study of CBPs, with a focus on polysialic acid (PSA), a polysaccharide found on neural stem cells.
31670365	7	38	theme	various	1392:1398	arg1	residues					1400:1407	various residues	1392:1407	various residues	1392:1407	Peptide compositional and positional analyses revealed the significance of various residues for PSA binding and suggested the importance of basic residue positioning for PSA recognition.
31670365	2	39	theme	natural	598:604	arg1	interactions					620:631	natural target/ligand interactions	598:631	natural target/ligand interactions	598:631	Amongst such ligands, carbohydrate-binding peptides (CBPs) are attractive for use in bioanalytical and biomedical systems due to their biochemical and physicochemical properties; moreover, given the biological significance of lectin-carbohydrate interactions, these ligands offer an opportunity to study peptide sequence and binding characteristics to inform on natural target/ligand interactions.
31670365	3	40	theme	screening	669:677	arg1	technique					679:687	a high-throughput microarray screening technique	640:687	a high-throughput microarray screening technique	640:687	Here, a high-throughput microarray screening technique is described for the identification and study of CBPs, with a focus on polysialic acid (PSA), a polysaccharide found on neural stem cells.
31670365	9	41	theme	peptides	1962:1969	arg1	study					1919:1923	study	1919:1923	study	1919:1923	Thus, the integrated approach described, with attention to design strategy, screening, and peptide characterization, successfully identified novel PSA-binding ligands and offers a platform for the identification and study of additional polysaccharide-binding peptides.
31670365	9	41	theme	peptides	1962:1969	arg1	identification					1900:1913	identification	1900:1913	identification	1900:1913	Thus, the integrated approach described, with attention to design strategy, screening, and peptide characterization, successfully identified novel PSA-binding ligands and offers a platform for the identification and study of additional polysaccharide-binding peptides.
31670365	5	42	theme	optimized	1125:1133	arg1	format					1146:1151	an optimized microarray format	1122:1151	an optimized microarray format	1122:1151	PSA-binding peptides from phage display screening and from epitope mapping of an scFv for oligosialic acid were screened in an optimized microarray format with three ligand density conditions.
31670365	2	43	theme	carbohydrate-binding	258:277	arg1	CBPs					289:292	CBPs	289:292	CBPs	289:292	Amongst such ligands, carbohydrate-binding peptides (CBPs) are attractive for use in bioanalytical and biomedical systems due to their biochemical and physicochemical properties; moreover, given the biological significance of lectin-carbohydrate interactions, these ligands offer an opportunity to study peptide sequence and binding characteristics to inform on natural target/ligand interactions.
31670365	2	43	theme	carbohydrate-binding	258:277	arg1	peptides					279:286	carbohydrate-binding peptides	258:286	carbohydrate-binding peptides (CBPs)	258:293	Amongst such ligands, carbohydrate-binding peptides (CBPs) are attractive for use in bioanalytical and biomedical systems due to their biochemical and physicochemical properties; moreover, given the biological significance of lectin-carbohydrate interactions, these ligands offer an opportunity to study peptide sequence and binding characteristics to inform on natural target/ligand interactions.
31670365	7	44	theme	positioning	1471:1481	arg1	importance					1443:1452	the importance	1439:1452	the importance of basic residue positioning for PSA recognition	1439:1501	Peptide compositional and positional analyses revealed the significance of various residues for PSA binding and suggested the importance of basic residue positioning for PSA recognition.
31670365	2	45	theme	biological	435:444	arg1	significance					446:457	the biological significance	431:457	the biological significance of lectin-carbohydrate interactions	431:493	Amongst such ligands, carbohydrate-binding peptides (CBPs) are attractive for use in bioanalytical and biomedical systems due to their biochemical and physicochemical properties; moreover, given the biological significance of lectin-carbohydrate interactions, these ligands offer an opportunity to study peptide sequence and binding characteristics to inform on natural target/ligand interactions.
31670365	0	46	theme	carbohydrate-binding	52:71	arg1	peptides					73:80	carbohydrate-binding peptides	52:80	carbohydrate-binding peptides	52:80	Microarrays for the screening and identification of carbohydrate-binding peptides.
31670365	9	47	theme	additional	1928:1937	arg1	peptides					1962:1969	additional polysaccharide-binding peptides	1928:1969	additional polysaccharide-binding peptides	1928:1969	Thus, the integrated approach described, with attention to design strategy, screening, and peptide characterization, successfully identified novel PSA-binding ligands and offers a platform for the identification and study of additional polysaccharide-binding peptides.
31670365	5	48	theme	microarray	1135:1144	arg1	format					1146:1151	an optimized microarray format	1122:1151	an optimized microarray format	1122:1151	PSA-binding peptides from phage display screening and from epitope mapping of an scFv for oligosialic acid were screened in an optimized microarray format with three ligand density conditions.
31670365	3	49	theme	high-throughput	642:656	arg1	screening					669:677	a high-throughput microarray screening	640:677	a high-throughput microarray screening technique	640:687	Here, a high-throughput microarray screening technique is described for the identification and study of CBPs, with a focus on polysialic acid (PSA), a polysaccharide found on neural stem cells.
31670365	2	50	theme	biochemical	371:381	arg1	properties					403:412	their biochemical and physicochemical properties	365:412	their biochemical and physicochemical properties	365:412	Amongst such ligands, carbohydrate-binding peptides (CBPs) are attractive for use in bioanalytical and biomedical systems due to their biochemical and physicochemical properties; moreover, given the biological significance of lectin-carbohydrate interactions, these ligands offer an opportunity to study peptide sequence and binding characteristics to inform on natural target/ligand interactions.
31670365	3	51	theme	neural	809:814	arg1	cells					821:825	neural stem cells	809:825	neural stem cells	809:825	Here, a high-throughput microarray screening technique is described for the identification and study of CBPs, with a focus on polysialic acid (PSA), a polysaccharide found on neural stem cells.
31670365	3	52	located	found	800:804	arg2	acid					771:774	polysialic acid	760:774	polysialic acid (PSA)	760:780	Here, a high-throughput microarray screening technique is described for the identification and study of CBPs, with a focus on polysialic acid (PSA), a polysaccharide found on neural stem cells.
31670365	3	52	located	found	800:804	arg1	cells					821:825	neural stem cells	809:825	neural stem cells	809:825	Here, a high-throughput microarray screening technique is described for the identification and study of CBPs, with a focus on polysialic acid (PSA), a polysaccharide found on neural stem cells.
31670365	3	52	located	found	800:804	arg2	polysaccharide					785:798	a polysaccharide	783:798	a polysaccharide found on neural stem cells	783:825	Here, a high-throughput microarray screening technique is described for the identification and study of CBPs, with a focus on polysialic acid (PSA), a polysaccharide found on neural stem cells.
31670365	2	53	theme	such	244:247	arg1	ligands					249:255	such ligands	244:255	such ligands	244:255	Amongst such ligands, carbohydrate-binding peptides (CBPs) are attractive for use in bioanalytical and biomedical systems due to their biochemical and physicochemical properties; moreover, given the biological significance of lectin-carbohydrate interactions, these ligands offer an opportunity to study peptide sequence and binding characteristics to inform on natural target/ligand interactions.
31670365	3	54	theme	microarray	658:667	arg1	screening					669:677	a high-throughput microarray screening	640:677	a high-throughput microarray screening technique	640:687	Here, a high-throughput microarray screening technique is described for the identification and study of CBPs, with a focus on polysialic acid (PSA), a polysaccharide found on neural stem cells.
31670365	8	55	theme	chondroitin	1576:1586	arg1	A					1596:1596	chondroitin sulfate A	1576:1596	chondroitin sulfate A (CS-A)	1576:1603	Furthermore, selectivity studies performed directly on microarrays with chondroitin sulfate A (CS-A) demonstrated the value of screening for both affinity and selectivity in the development of CBPs.
31670365	8	55	theme	chondroitin	1576:1586	arg1	CS-A					1599:1602	CS-A	1599:1602	CS-A	1599:1602	Furthermore, selectivity studies performed directly on microarrays with chondroitin sulfate A (CS-A) demonstrated the value of screening for both affinity and selectivity in the development of CBPs.
31670365	3	56	theme	stem	816:819	arg1	cells					821:825	neural stem cells	809:825	neural stem cells	809:825	Here, a high-throughput microarray screening technique is described for the identification and study of CBPs, with a focus on polysialic acid (PSA), a polysaccharide found on neural stem cells.
31670365	9	57	theme	novel	1844:1848	arg1	ligands					1862:1868	novel PSA-binding ligands	1844:1868	novel PSA-binding ligands	1844:1868	Thus, the integrated approach described, with attention to design strategy, screening, and peptide characterization, successfully identified novel PSA-binding ligands and offers a platform for the identification and study of additional polysaccharide-binding peptides.
31670365	4	58	theme	chemical	832:839	arg1	uniqueness					856:865	The chemical and biological uniqueness	828:865	The chemical and biological uniqueness of PSA	828:872	The chemical and biological uniqueness of PSA suggests that an ability to exclusively target this glycan may promote a number of diagnostic and therapeutic applications.
31670365	0	59	dep	screening	20:28	arg1	the					16:18	the	16:18	the	16:18	Microarrays for the screening and identification of carbohydrate-binding peptides.
31670365	7	60	theme	PSA	1487:1489	arg1	recognition					1491:1501	PSA recognition	1487:1501	PSA recognition	1487:1501	Peptide compositional and positional analyses revealed the significance of various residues for PSA binding and suggested the importance of basic residue positioning for PSA recognition.
31670365	8	61	with	microarrays	1559:1569	arg1	A					1596:1596	chondroitin sulfate A	1576:1596	chondroitin sulfate A (CS-A)	1576:1603	Furthermore, selectivity studies performed directly on microarrays with chondroitin sulfate A (CS-A) demonstrated the value of screening for both affinity and selectivity in the development of CBPs.
31670365	8	61	with	microarrays	1559:1569	arg1	CS-A					1599:1602	CS-A	1599:1602	CS-A	1599:1602	Furthermore, selectivity studies performed directly on microarrays with chondroitin sulfate A (CS-A) demonstrated the value of screening for both affinity and selectivity in the development of CBPs.
31670365	5	62	theme	PSA-binding	998:1008	arg1	peptides					1010:1017	PSA-binding peptides	998:1017	PSA-binding peptides from phage display screening and from epitope mapping of an scFv for oligosialic acid	998:1103	PSA-binding peptides from phage display screening and from epitope mapping of an scFv for oligosialic acid were screened in an optimized microarray format with three ligand density conditions.
31670365	3	63	dep	identification	710:723	arg1	the					706:708	the	706:708	the	706:708	Here, a high-throughput microarray screening technique is described for the identification and study of CBPs, with a focus on polysialic acid (PSA), a polysaccharide found on neural stem cells.
31670365	9	64	theme	PSA-binding	1850:1860	arg1	ligands					1862:1868	novel PSA-binding ligands	1844:1868	novel PSA-binding ligands	1844:1868	Thus, the integrated approach described, with attention to design strategy, screening, and peptide characterization, successfully identified novel PSA-binding ligands and offers a platform for the identification and study of additional polysaccharide-binding peptides.
31670365	3	65	theme	CBPs	738:741	arg1	study					729:733	study	729:733	study	729:733	Here, a high-throughput microarray screening technique is described for the identification and study of CBPs, with a focus on polysialic acid (PSA), a polysaccharide found on neural stem cells.
31670365	3	65	theme	CBPs	738:741	arg1	identification					710:723	identification	710:723	identification	710:723	Here, a high-throughput microarray screening technique is described for the identification and study of CBPs, with a focus on polysialic acid (PSA), a polysaccharide found on neural stem cells.
31670365	7	66	theme	basic	1457:1461	arg1	positioning					1471:1481	basic residue positioning	1457:1481	basic residue positioning	1457:1481	Peptide compositional and positional analyses revealed the significance of various residues for PSA binding and suggested the importance of basic residue positioning for PSA recognition.
31670365	6	67	theme	select	1248:1253	arg1	peptides					1255:1262	select peptides	1248:1262	select peptides	1248:1262	Hypothesis-driven mutations were additionally applied to select peptides to modulate peptide affinity and selectivity to PSA.
31670365	2	68	theme	due	358:360	arg1	systems					350:356	bioanalytical and biomedical systems	321:356	bioanalytical and biomedical systems due to their biochemical and physicochemical properties	321:412	Amongst such ligands, carbohydrate-binding peptides (CBPs) are attractive for use in bioanalytical and biomedical systems due to their biochemical and physicochemical properties; moreover, given the biological significance of lectin-carbohydrate interactions, these ligands offer an opportunity to study peptide sequence and binding characteristics to inform on natural target/ligand interactions.
31670365	7	69	theme	PSA	1413:1415	arg1	binding					1417:1423	PSA binding	1413:1423	PSA binding	1413:1423	Peptide compositional and positional analyses revealed the significance of various residues for PSA binding and suggested the importance of basic residue positioning for PSA recognition.
31670365	5	70	theme	display	1030:1036	arg1	screening					1038:1046	phage display screening	1024:1046	phage display screening	1024:1046	PSA-binding peptides from phage display screening and from epitope mapping of an scFv for oligosialic acid were screened in an optimized microarray format with three ligand density conditions.
31670365	4	71	theme	biological	845:854	arg1	uniqueness					856:865	The chemical and biological uniqueness	828:865	The chemical and biological uniqueness of PSA	828:872	The chemical and biological uniqueness of PSA suggests that an ability to exclusively target this glycan may promote a number of diagnostic and therapeutic applications.
31670365	7	72	theme	residues	1400:1407	arg1	significance					1376:1387	the significance	1372:1387	the significance of various residues for PSA binding	1372:1423	Peptide compositional and positional analyses revealed the significance of various residues for PSA binding and suggested the importance of basic residue positioning for PSA recognition.
31670365	2	73	from	use	314:316	arg1	systems					350:356	bioanalytical and biomedical systems	321:356	bioanalytical and biomedical systems due to their biochemical and physicochemical properties	321:412	Amongst such ligands, carbohydrate-binding peptides (CBPs) are attractive for use in bioanalytical and biomedical systems due to their biochemical and physicochemical properties; moreover, given the biological significance of lectin-carbohydrate interactions, these ligands offer an opportunity to study peptide sequence and binding characteristics to inform on natural target/ligand interactions.
31670365	0	74	theme	peptides	73:80	arg1	identification					34:47	identification	34:47	identification	34:47	Microarrays for the screening and identification of carbohydrate-binding peptides.
31670365	0	74	theme	peptides	73:80	arg1	screening					20:28	screening	20:28	screening	20:28	Microarrays for the screening and identification of carbohydrate-binding peptides.
31670365	3	75	theme	polysialic	760:769	arg1	PSA					777:779	PSA	777:779	PSA	777:779	Here, a high-throughput microarray screening technique is described for the identification and study of CBPs, with a focus on polysialic acid (PSA), a polysaccharide found on neural stem cells.
31670365	3	75	theme	polysialic	760:769	arg1	acid					771:774	polysialic acid	760:774	polysialic acid (PSA)	760:780	Here, a high-throughput microarray screening technique is described for the identification and study of CBPs, with a focus on polysialic acid (PSA), a polysaccharide found on neural stem cells.
31670365	3	75	theme	polysialic	760:769	arg1	polysaccharide					785:798	a polysaccharide	783:798	a polysaccharide found on neural stem cells	783:825	Here, a high-throughput microarray screening technique is described for the identification and study of CBPs, with a focus on polysialic acid (PSA), a polysaccharide found on neural stem cells.
31670365	2	76	theme	interactions	482:493	arg1	significance					446:457	the biological significance	431:457	the biological significance of lectin-carbohydrate interactions	431:493	Amongst such ligands, carbohydrate-binding peptides (CBPs) are attractive for use in bioanalytical and biomedical systems due to their biochemical and physicochemical properties; moreover, given the biological significance of lectin-carbohydrate interactions, these ligands offer an opportunity to study peptide sequence and binding characteristics to inform on natural target/ligand interactions.
31670365	9	77	dep	strategy	1769:1776	arg1	attention					1749:1757	attention	1749:1757	attention	1749:1757	Thus, the integrated approach described, with attention to design strategy, screening, and peptide characterization, successfully identified novel PSA-binding ligands and offers a platform for the identification and study of additional polysaccharide-binding peptides.
31670365	7	78	theme	positional	1343:1352	arg1	analyses					1354:1361	Peptide compositional and positional analyses	1317:1361	analyses	1354:1361	Peptide compositional and positional analyses revealed the significance of various residues for PSA binding and suggested the importance of basic residue positioning for PSA recognition.
31670365	7	79	theme	Peptide	1317:1323	arg1	compositional					1325:1337	Peptide compositional and positional analyses	1317:1361	compositional	1325:1337	Peptide compositional and positional analyses revealed the significance of various residues for PSA binding and suggested the importance of basic residue positioning for PSA recognition.
29802187	10	0	theme	keystone	2296:2303	arg1	species					2305:2311	a keystone species	2294:2311	a keystone species	2294:2311	B. pseudolongum has the characteristics of a keystone species in the community because it had low abundance but high ability to perform a critical function, the hydrolysis of resistant starch.
29802187	6	1	theme	maltose	1097:1103	arg1	medium					1105:1110	maltose medium	1097:1110	maltose medium	1097:1110	Maltose was released into the culture medium, and B. animalis cultures had shorter doubling times in maltose medium than did B. pseudolongum Thus, B. pseudolongum, which was present at a consistently low abundance in the microbiota, but which has extensive enzymatic capacity to degrade resistant starch, showed the attributes of a keystone species associated with the bifidobacterial bloom.IMPORTANCE This study addresses the microbiology and function of a natural ecosystem (the rat gut) using DNA-based observations and in vitro experimentation.
29802187	9	2	theme	DNA	1902:1904	arg1	analysis					1915:1922	DNA sequence analysis	1902:1922	DNA sequence analysis	1902:1922	Our work, combining DNA sequence analysis and laboratory experiments with cultured strains of bacteria, revealed that the increased abundance of bifidobacteria in the rat gut, induced by feeding indigestible starch, involved a species that cannot itself degrade the starch (Bifidobacterium animalis) but cohabits with a species that can (Bifidobacterium pseudolongum).
29802187	6	3	located	present	1170:1176	arg1	abundance					1200:1208	a consistently low abundance	1181:1208	a consistently low abundance in the microbiota	1181:1226	Maltose was released into the culture medium, and B. animalis cultures had shorter doubling times in maltose medium than did B. pseudolongum Thus, B. pseudolongum, which was present at a consistently low abundance in the microbiota, but which has extensive enzymatic capacity to degrade resistant starch, showed the attributes of a keystone species associated with the bifidobacterial bloom.IMPORTANCE This study addresses the microbiology and function of a natural ecosystem (the rat gut) using DNA-based observations and in vitro experimentation.
29802187	6	3	located	present	1170:1176	arg2	which					1160:1164	which	1160:1164	which	1160:1164	Maltose was released into the culture medium, and B. animalis cultures had shorter doubling times in maltose medium than did B. pseudolongum Thus, B. pseudolongum, which was present at a consistently low abundance in the microbiota, but which has extensive enzymatic capacity to degrade resistant starch, showed the attributes of a keystone species associated with the bifidobacterial bloom.IMPORTANCE This study addresses the microbiology and function of a natural ecosystem (the rat gut) using DNA-based observations and in vitro experimentation.
29802187	3	4	from	present	484:490	arg1	cecum					503:507	the rat cecum	495:507	the rat cecum under these circumstances	495:533	We determined that the bifidobacterial species present in the rat cecum under these circumstances mostly belonged to Bifidobacterium animalis However, cultures of B. animalis isolated from the rats failed to degrade Hi-Maize starch to any extent.
29802187	2	5	theme	bifidobacteria	326:339	arg1	abundances					312:321	relative abundances	303:321	relative abundances of bifidobacteria (blooms)	303:348	Increases in relative abundances of bifidobacteria (blooms) have been reported in rats whose diet was supplemented with Hi-Maize resistant starch.
29802187	6	6	theme	ecosystem	1462:1470	arg1	function					1440:1447	function	1440:1447	function	1440:1447	Maltose was released into the culture medium, and B. animalis cultures had shorter doubling times in maltose medium than did B. pseudolongum Thus, B. pseudolongum, which was present at a consistently low abundance in the microbiota, but which has extensive enzymatic capacity to degrade resistant starch, showed the attributes of a keystone species associated with the bifidobacterial bloom.IMPORTANCE This study addresses the microbiology and function of a natural ecosystem (the rat gut) using DNA-based observations and in vitro experimentation.
29802187	6	6	theme	ecosystem	1462:1470	arg1	microbiology					1423:1434	the microbiology	1419:1434	the microbiology	1419:1434	Maltose was released into the culture medium, and B. animalis cultures had shorter doubling times in maltose medium than did B. pseudolongum Thus, B. pseudolongum, which was present at a consistently low abundance in the microbiota, but which has extensive enzymatic capacity to degrade resistant starch, showed the attributes of a keystone species associated with the bifidobacterial bloom.IMPORTANCE This study addresses the microbiology and function of a natural ecosystem (the rat gut) using DNA-based observations and in vitro experimentation.
29802187	6	7	theme	enzymatic	1253:1261	arg1	capacity					1263:1270	extensive enzymatic capacity	1243:1270	extensive enzymatic capacity to degrade resistant starch, showed the attributes of a keystone species associated with the bifidobacterial bloom.IMPORTANCE This study addresses the microbiology and function of a natural ecosystem (the rat gut) using DNA-based observations and in vitro experimentation	1243:1542	Maltose was released into the culture medium, and B. animalis cultures had shorter doubling times in maltose medium than did B. pseudolongum Thus, B. pseudolongum, which was present at a consistently low abundance in the microbiota, but which has extensive enzymatic capacity to degrade resistant starch, showed the attributes of a keystone species associated with the bifidobacterial bloom.IMPORTANCE This study addresses the microbiology and function of a natural ecosystem (the rat gut) using DNA-based observations and in vitro experimentation.
29802187	6	8	dep	degrade	1275:1281	arg1	showed					1301:1306	showed	1301:1306	showed the attributes of a keystone species associated with the bifidobacterial bloom.IMPORTANCE This study addresses the microbiology and function of a natural ecosystem (the rat gut) using DNA-based observations and in vitro experimentation	1301:1542	Maltose was released into the culture medium, and B. animalis cultures had shorter doubling times in maltose medium than did B. pseudolongum Thus, B. pseudolongum, which was present at a consistently low abundance in the microbiota, but which has extensive enzymatic capacity to degrade resistant starch, showed the attributes of a keystone species associated with the bifidobacterial bloom.IMPORTANCE This study addresses the microbiology and function of a natural ecosystem (the rat gut) using DNA-based observations and in vitro experimentation.
29802187	9	9	theme	laboratory	1928:1937	arg1	experiments					1939:1949	laboratory experiments	1928:1949	laboratory experiments with cultured strains of bacteria	1928:1983	Our work, combining DNA sequence analysis and laboratory experiments with cultured strains of bacteria, revealed that the increased abundance of bifidobacteria in the rat gut, induced by feeding indigestible starch, involved a species that cannot itself degrade the starch (Bifidobacterium animalis) but cohabits with a species that can (Bifidobacterium pseudolongum).
29802187	10	10	theme	starch	2436:2441	arg1	hydrolysis					2412:2421	the hydrolysis	2408:2421	the hydrolysis of resistant starch	2408:2441	B. pseudolongum has the characteristics of a keystone species in the community because it had low abundance but high ability to perform a critical function, the hydrolysis of resistant starch.
29802187	5	11	theme	pullulanase	865:875	arg1	expression					842:851	increased expression	832:851	increased expression of a type 1 pullulanase, alpha-amylase, and glycogen debranching enzyme by B. pseudolongum	832:942	Transcriptional comparisons showed increased expression of a type 1 pullulanase, alpha-amylase, and glycogen debranching enzyme by B. pseudolongum when cultured in medium containing Hi-Maize starch.
29802187	5	12	contain	containing	968:977	arg1	medium					961:966	medium	961:966	medium containing Hi-Maize starch	961:993	Transcriptional comparisons showed increased expression of a type 1 pullulanase, alpha-amylase, and glycogen debranching enzyme by B. pseudolongum when cultured in medium containing Hi-Maize starch.
29802187	5	12	contain	containing	968:977	arg2	starch					988:993	Hi-Maize starch	979:993	Hi-Maize starch	979:993	Transcriptional comparisons showed increased expression of a type 1 pullulanase, alpha-amylase, and glycogen debranching enzyme by B. pseudolongum when cultured in medium containing Hi-Maize starch.
29802187	6	13	theme	in	1519:1520	arg1	experimentation					1528:1542	in vitro experimentation	1519:1542	in vitro experimentation	1519:1542	Maltose was released into the culture medium, and B. animalis cultures had shorter doubling times in maltose medium than did B. pseudolongum Thus, B. pseudolongum, which was present at a consistently low abundance in the microbiota, but which has extensive enzymatic capacity to degrade resistant starch, showed the attributes of a keystone species associated with the bifidobacterial bloom.IMPORTANCE This study addresses the microbiology and function of a natural ecosystem (the rat gut) using DNA-based observations and in vitro experimentation.
29802187	5	14	theme	alpha-amylase	878:890	arg1	expression					842:851	increased expression	832:851	increased expression of a type 1 pullulanase, alpha-amylase, and glycogen debranching enzyme by B. pseudolongum	832:942	Transcriptional comparisons showed increased expression of a type 1 pullulanase, alpha-amylase, and glycogen debranching enzyme by B. pseudolongum when cultured in medium containing Hi-Maize starch.
29802187	9	15	dep	starch	2148:2153	arg1	animalis					2172:2179	Bifidobacterium animalis	2156:2179	Bifidobacterium animalis	2156:2179	Our work, combining DNA sequence analysis and laboratory experiments with cultured strains of bacteria, revealed that the increased abundance of bifidobacteria in the rat gut, induced by feeding indigestible starch, involved a species that cannot itself degrade the starch (Bifidobacterium animalis) but cohabits with a species that can (Bifidobacterium pseudolongum).
29802187	10	16	theme	low	2345:2347	arg1	abundance					2349:2357	low abundance	2345:2357	low abundance	2345:2357	B. pseudolongum has the characteristics of a keystone species in the community because it had low abundance but high ability to perform a critical function, the hydrolysis of resistant starch.
29802187	1	17	from	foods	195:199	arg1	present					184:190	present	184:190	present	184:190	Starches resistant to mammalian digestion are present in foods and pass to the large bowel, where they may be degraded and fermented by the microbiota.
29802187	9	18	dep	degrade	2136:2142	arg1	species					2109:2115	a species	2107:2115	a species that cannot itself degrade the starch (Bifidobacterium animalis) but cohabits with a species that can (Bifidobacterium pseudolongum)	2107:2248	Our work, combining DNA sequence analysis and laboratory experiments with cultured strains of bacteria, revealed that the increased abundance of bifidobacteria in the rat gut, induced by feeding indigestible starch, involved a species that cannot itself degrade the starch (Bifidobacterium animalis) but cohabits with a species that can (Bifidobacterium pseudolongum).
29802187	9	19	theme	sequence	1906:1913	arg1	analysis					1915:1922	DNA sequence analysis	1902:1922	DNA sequence analysis	1902:1922	Our work, combining DNA sequence analysis and laboratory experiments with cultured strains of bacteria, revealed that the increased abundance of bifidobacteria in the rat gut, induced by feeding indigestible starch, involved a species that cannot itself degrade the starch (Bifidobacterium animalis) but cohabits with a species that can (Bifidobacterium pseudolongum).
29802187	9	20	theme	bacteria	1976:1983	arg1	strains					1965:1971	cultured strains	1956:1971	cultured strains of bacteria	1956:1983	Our work, combining DNA sequence analysis and laboratory experiments with cultured strains of bacteria, revealed that the increased abundance of bifidobacteria in the rat gut, induced by feeding indigestible starch, involved a species that cannot itself degrade the starch (Bifidobacterium animalis) but cohabits with a species that can (Bifidobacterium pseudolongum).
29802187	5	21	theme	type	858:861	arg1	pullulanase					865:875	a type 1 pullulanase	856:875	a type 1 pullulanase	856:875	Transcriptional comparisons showed increased expression of a type 1 pullulanase, alpha-amylase, and glycogen debranching enzyme by B. pseudolongum when cultured in medium containing Hi-Maize starch.
29802187	8	22	theme	genetic	1785:1791	arg1	capacities					1793:1802	genetic capacities	1785:1802	genetic capacities	1785:1802	These studies reveal the compositions and genetic capacities of microbiotas but not the intricacies of how microbial communities function.
29802187	3	23	attach	isolated	612:619	arg1	rats					630:633	the rats	626:633	the rats	626:633	We determined that the bifidobacterial species present in the rat cecum under these circumstances mostly belonged to Bifidobacterium animalis However, cultures of B. animalis isolated from the rats failed to degrade Hi-Maize starch to any extent.
29802187	3	23	attach	isolated	612:619	arg2	animalis					603:610	B. animalis	600:610	B. animalis isolated from the rats	600:633	We determined that the bifidobacterial species present in the rat cecum under these circumstances mostly belonged to Bifidobacterium animalis However, cultures of B. animalis isolated from the rats failed to degrade Hi-Maize starch to any extent.
29802187	4	24	theme	Bifidobacterium	697:711	arg1	pseudolongum					713:724	Bifidobacterium pseudolongum	697:724	Bifidobacterium pseudolongum also detected in the rat microbiota	697:760	In contrast, Bifidobacterium pseudolongum also detected in the rat microbiota had high starch-degrading ability.
29802187	6	25	theme	resistant	1283:1291	arg1	starch					1293:1298	resistant starch	1283:1298	resistant starch	1283:1298	Maltose was released into the culture medium, and B. animalis cultures had shorter doubling times in maltose medium than did B. pseudolongum Thus, B. pseudolongum, which was present at a consistently low abundance in the microbiota, but which has extensive enzymatic capacity to degrade resistant starch, showed the attributes of a keystone species associated with the bifidobacterial bloom.IMPORTANCE This study addresses the microbiology and function of a natural ecosystem (the rat gut) using DNA-based observations and in vitro experimentation.
29802187	1	26	from	present	184:190	arg1	foods					195:199	foods	195:199	foods	195:199	Starches resistant to mammalian digestion are present in foods and pass to the large bowel, where they may be degraded and fermented by the microbiota.
29802187	7	27	theme	animals	1591:1597	arg1	bowel					1582:1586	the large bowel	1572:1586	the large bowel of animals, including humans,	1572:1616	The microbial community of the large bowel of animals, including humans, has been studied extensively through the use of high-throughput DNA sequencing methods and advanced bioinformatics analysis.
29802187	10	28	theme	critical	2389:2396	arg1	function					2398:2405	a critical function	2387:2405	a critical function	2387:2405	B. pseudolongum has the characteristics of a keystone species in the community because it had low abundance but high ability to perform a critical function, the hydrolysis of resistant starch.
29802187	0	29	theme	Hi-Maize	53:60	arg1	Starch					62:67	Hi-Maize Starch	53:67	Hi-Maize Starch	53:67	Bifidobacterium pseudolongum in the Ceca of Rats Fed Hi-Maize Starch Has Characteristics of a Keystone Species in Bifidobacterial Blooms.
29802187	1	30	theme	resistant	147:155	arg1	Starches					138:145	Starches	138:145	Starches resistant to mammalian digestion	138:178	Starches resistant to mammalian digestion are present in foods and pass to the large bowel, where they may be degraded and fermented by the microbiota.
29802187	7	31	theme	bioinformatics	1718:1731	arg1	analysis					1733:1740	advanced bioinformatics analysis	1709:1740	advanced bioinformatics analysis	1709:1740	The microbial community of the large bowel of animals, including humans, has been studied extensively through the use of high-throughput DNA sequencing methods and advanced bioinformatics analysis.
29802187	7	32	theme	bowel	1582:1586	arg1	community					1559:1567	The microbial community	1545:1567	The microbial community of the large bowel of animals, including humans,	1545:1616	The microbial community of the large bowel of animals, including humans, has been studied extensively through the use of high-throughput DNA sequencing methods and advanced bioinformatics analysis.
29802187	9	33	theme	bifidobacteria	2027:2040	arg1	abundance					2014:2022	the increased abundance	2000:2022	the increased abundance of bifidobacteria in the rat gut, induced by feeding indigestible starch,	2000:2096	Our work, combining DNA sequence analysis and laboratory experiments with cultured strains of bacteria, revealed that the increased abundance of bifidobacteria in the rat gut, induced by feeding indigestible starch, involved a species that cannot itself degrade the starch (Bifidobacterium animalis) but cohabits with a species that can (Bifidobacterium pseudolongum).
29802187	6	34	attach	present	1170:1176	arg1	abundance					1200:1208	a consistently low abundance	1181:1208	a consistently low abundance in the microbiota	1181:1226	Maltose was released into the culture medium, and B. animalis cultures had shorter doubling times in maltose medium than did B. pseudolongum Thus, B. pseudolongum, which was present at a consistently low abundance in the microbiota, but which has extensive enzymatic capacity to degrade resistant starch, showed the attributes of a keystone species associated with the bifidobacterial bloom.IMPORTANCE This study addresses the microbiology and function of a natural ecosystem (the rat gut) using DNA-based observations and in vitro experimentation.
29802187	6	34	attach	present	1170:1176	arg2	which					1160:1164	which	1160:1164	which	1160:1164	Maltose was released into the culture medium, and B. animalis cultures had shorter doubling times in maltose medium than did B. pseudolongum Thus, B. pseudolongum, which was present at a consistently low abundance in the microbiota, but which has extensive enzymatic capacity to degrade resistant starch, showed the attributes of a keystone species associated with the bifidobacterial bloom.IMPORTANCE This study addresses the microbiology and function of a natural ecosystem (the rat gut) using DNA-based observations and in vitro experimentation.
29802187	5	35	theme	debranching	906:916	arg1	enzyme					918:923	glycogen debranching enzyme	897:923	glycogen debranching enzyme	897:923	Transcriptional comparisons showed increased expression of a type 1 pullulanase, alpha-amylase, and glycogen debranching enzyme by B. pseudolongum when cultured in medium containing Hi-Maize starch.
29802187	2	36	from	Increases	290:298	arg1	abundances					312:321	relative abundances	303:321	relative abundances of bifidobacteria (blooms)	303:348	Increases in relative abundances of bifidobacteria (blooms) have been reported in rats whose diet was supplemented with Hi-Maize resistant starch.
29802187	4	37	theme	rat	747:749	arg1	microbiota					751:760	the rat microbiota	743:760	the rat microbiota	743:760	In contrast, Bifidobacterium pseudolongum also detected in the rat microbiota had high starch-degrading ability.
29802187	10	38	contain	has	2267:2269	arg1	pseudolongum					2254:2265	B. pseudolongum	2251:2265	B. pseudolongum	2251:2265	B. pseudolongum has the characteristics of a keystone species in the community because it had low abundance but high ability to perform a critical function, the hydrolysis of resistant starch.
29802187	10	38	contain	has	2267:2269	arg2	characteristics					2275:2289	the characteristics	2271:2289	the characteristics of a keystone species	2271:2311	B. pseudolongum has the characteristics of a keystone species in the community because it had low abundance but high ability to perform a critical function, the hydrolysis of resistant starch.
29802187	5	39	theme	Hi-Maize	979:986	arg1	starch					988:993	Hi-Maize starch	979:993	Hi-Maize starch	979:993	Transcriptional comparisons showed increased expression of a type 1 pullulanase, alpha-amylase, and glycogen debranching enzyme by B. pseudolongum when cultured in medium containing Hi-Maize starch.
29802187	7	40	theme	sequencing	1686:1695	arg1	use					1659:1661	the use	1655:1661	the use of high-throughput DNA sequencing methods and advanced bioinformatics analysis	1655:1740	The microbial community of the large bowel of animals, including humans, has been studied extensively through the use of high-throughput DNA sequencing methods and advanced bioinformatics analysis.
29802187	6	41	theme	keystone	1328:1335	arg1	species					1337:1343	a keystone species	1326:1343	a keystone species associated with the bifidobacterial bloom.IMPORTANCE This study addresses the microbiology and function of a natural ecosystem (the rat gut) using DNA-based observations and in vitro experimentation	1326:1542	Maltose was released into the culture medium, and B. animalis cultures had shorter doubling times in maltose medium than did B. pseudolongum Thus, B. pseudolongum, which was present at a consistently low abundance in the microbiota, but which has extensive enzymatic capacity to degrade resistant starch, showed the attributes of a keystone species associated with the bifidobacterial bloom.IMPORTANCE This study addresses the microbiology and function of a natural ecosystem (the rat gut) using DNA-based observations and in vitro experimentation.
29802187	8	42	theme	microbial	1850:1858	arg1	communities					1860:1870	microbial communities	1850:1870	microbial communities	1850:1870	These studies reveal the compositions and genetic capacities of microbiotas but not the intricacies of how microbial communities function.
29802187	7	43	theme	high-throughput	1666:1680	arg1	sequencing					1686:1695	high-throughput DNA sequencing	1666:1695	high-throughput DNA sequencing	1666:1695	The microbial community of the large bowel of animals, including humans, has been studied extensively through the use of high-throughput DNA sequencing methods and advanced bioinformatics analysis.
29802187	6	44	theme	doubling	1079:1086	arg1	times					1088:1092	shorter doubling times	1071:1092	shorter doubling times	1071:1092	Maltose was released into the culture medium, and B. animalis cultures had shorter doubling times in maltose medium than did B. pseudolongum Thus, B. pseudolongum, which was present at a consistently low abundance in the microbiota, but which has extensive enzymatic capacity to degrade resistant starch, showed the attributes of a keystone species associated with the bifidobacterial bloom.IMPORTANCE This study addresses the microbiology and function of a natural ecosystem (the rat gut) using DNA-based observations and in vitro experimentation.
29802187	2	45	theme	resistant	419:427	arg1	starch					429:434	Hi-Maize resistant starch	410:434	Hi-Maize resistant starch	410:434	Increases in relative abundances of bifidobacteria (blooms) have been reported in rats whose diet was supplemented with Hi-Maize resistant starch.
29802187	0	46	theme	Bifidobacterium	0:14	arg1	pseudolongum					16:27	Bifidobacterium pseudolongum	0:27	Bifidobacterium pseudolongum in the Ceca of Rats Fed Hi-Maize Starch	0:67	Bifidobacterium pseudolongum in the Ceca of Rats Fed Hi-Maize Starch Has Characteristics of a Keystone Species in Bifidobacterial Blooms.
29802187	6	47	dep	bloom.IMPORTANCE	1381:1396	arg1	addresses					1409:1417	addresses	1409:1417	addresses the microbiology and function of a natural ecosystem (the rat gut) using DNA-based observations and in vitro experimentation	1409:1542	Maltose was released into the culture medium, and B. animalis cultures had shorter doubling times in maltose medium than did B. pseudolongum Thus, B. pseudolongum, which was present at a consistently low abundance in the microbiota, but which has extensive enzymatic capacity to degrade resistant starch, showed the attributes of a keystone species associated with the bifidobacterial bloom.IMPORTANCE This study addresses the microbiology and function of a natural ecosystem (the rat gut) using DNA-based observations and in vitro experimentation.
29802187	9	48	from	abundance	2014:2022	arg1	gut					2053:2055	the rat gut	2045:2055	the rat gut	2045:2055	Our work, combining DNA sequence analysis and laboratory experiments with cultured strains of bacteria, revealed that the increased abundance of bifidobacteria in the rat gut, induced by feeding indigestible starch, involved a species that cannot itself degrade the starch (Bifidobacterium animalis) but cohabits with a species that can (Bifidobacterium pseudolongum).
29802187	10	49	theme	resistant	2426:2434	arg1	starch					2436:2441	resistant starch	2426:2441	resistant starch	2426:2441	B. pseudolongum has the characteristics of a keystone species in the community because it had low abundance but high ability to perform a critical function, the hydrolysis of resistant starch.
29802187	9	50	with	cohabits	2186:2193	arg1	species					2202:2208	a species	2200:2208	a species that can (Bifidobacterium pseudolongum)	2200:2248	Our work, combining DNA sequence analysis and laboratory experiments with cultured strains of bacteria, revealed that the increased abundance of bifidobacteria in the rat gut, induced by feeding indigestible starch, involved a species that cannot itself degrade the starch (Bifidobacterium animalis) but cohabits with a species that can (Bifidobacterium pseudolongum).
29802187	5	51	theme	increased	832:840	arg1	expression					842:851	increased expression	832:851	increased expression of a type 1 pullulanase, alpha-amylase, and glycogen debranching enzyme by B. pseudolongum	832:942	Transcriptional comparisons showed increased expression of a type 1 pullulanase, alpha-amylase, and glycogen debranching enzyme by B. pseudolongum when cultured in medium containing Hi-Maize starch.
29802187	6	52	contain	has	1239:1241	arg2	capacity					1263:1270	extensive enzymatic capacity	1243:1270	extensive enzymatic capacity to degrade resistant starch, showed the attributes of a keystone species associated with the bifidobacterial bloom.IMPORTANCE This study addresses the microbiology and function of a natural ecosystem (the rat gut) using DNA-based observations and in vitro experimentation	1243:1542	Maltose was released into the culture medium, and B. animalis cultures had shorter doubling times in maltose medium than did B. pseudolongum Thus, B. pseudolongum, which was present at a consistently low abundance in the microbiota, but which has extensive enzymatic capacity to degrade resistant starch, showed the attributes of a keystone species associated with the bifidobacterial bloom.IMPORTANCE This study addresses the microbiology and function of a natural ecosystem (the rat gut) using DNA-based observations and in vitro experimentation.
29802187	6	52	contain	has	1239:1241	arg1	which					1233:1237	which	1233:1237	which	1233:1237	Maltose was released into the culture medium, and B. animalis cultures had shorter doubling times in maltose medium than did B. pseudolongum Thus, B. pseudolongum, which was present at a consistently low abundance in the microbiota, but which has extensive enzymatic capacity to degrade resistant starch, showed the attributes of a keystone species associated with the bifidobacterial bloom.IMPORTANCE This study addresses the microbiology and function of a natural ecosystem (the rat gut) using DNA-based observations and in vitro experimentation.
29802187	6	53	theme	culture	1026:1032	arg1	medium					1034:1039	the culture medium	1022:1039	the culture medium	1022:1039	Maltose was released into the culture medium, and B. animalis cultures had shorter doubling times in maltose medium than did B. pseudolongum Thus, B. pseudolongum, which was present at a consistently low abundance in the microbiota, but which has extensive enzymatic capacity to degrade resistant starch, showed the attributes of a keystone species associated with the bifidobacterial bloom.IMPORTANCE This study addresses the microbiology and function of a natural ecosystem (the rat gut) using DNA-based observations and in vitro experimentation.
29802187	1	54	attach	present	184:190	arg2	Starches					138:145	Starches	138:145	Starches resistant to mammalian digestion	138:178	Starches resistant to mammalian digestion are present in foods and pass to the large bowel, where they may be degraded and fermented by the microbiota.
29802187	1	54	attach	present	184:190	arg1	foods					195:199	foods	195:199	foods	195:199	Starches resistant to mammalian digestion are present in foods and pass to the large bowel, where they may be degraded and fermented by the microbiota.
29802187	3	55	theme	bifidobacterial	460:474	arg1	species					476:482	the bifidobacterial species	456:482	the bifidobacterial species present in the rat cecum under these circumstances	456:533	We determined that the bifidobacterial species present in the rat cecum under these circumstances mostly belonged to Bifidobacterium animalis However, cultures of B. animalis isolated from the rats failed to degrade Hi-Maize starch to any extent.
29802187	0	56	theme	Rats	44:47	arg1	Ceca					36:39	the Ceca	32:39	the Ceca of Rats Fed Hi-Maize Starch	32:67	Bifidobacterium pseudolongum in the Ceca of Rats Fed Hi-Maize Starch Has Characteristics of a Keystone Species in Bifidobacterial Blooms.
29802187	4	57	theme	starch-degrading	771:786	arg1	ability					788:794	high starch-degrading ability	766:794	high starch-degrading ability	766:794	In contrast, Bifidobacterium pseudolongum also detected in the rat microbiota had high starch-degrading ability.
29802187	2	58	theme	relative	303:310	arg1	abundances					312:321	relative abundances	303:321	relative abundances of bifidobacteria (blooms)	303:348	Increases in relative abundances of bifidobacteria (blooms) have been reported in rats whose diet was supplemented with Hi-Maize resistant starch.
29802187	6	59	theme	rat	1477:1479	arg1	gut					1481:1483	the rat gut	1473:1483	the rat gut	1473:1483	Maltose was released into the culture medium, and B. animalis cultures had shorter doubling times in maltose medium than did B. pseudolongum Thus, B. pseudolongum, which was present at a consistently low abundance in the microbiota, but which has extensive enzymatic capacity to degrade resistant starch, showed the attributes of a keystone species associated with the bifidobacterial bloom.IMPORTANCE This study addresses the microbiology and function of a natural ecosystem (the rat gut) using DNA-based observations and in vitro experimentation.
29802187	6	59	theme	rat	1477:1479	arg1	ecosystem					1462:1470	a natural ecosystem	1452:1470	a natural ecosystem (the rat gut) using DNA-based observations and in vitro experimentation	1452:1542	Maltose was released into the culture medium, and B. animalis cultures had shorter doubling times in maltose medium than did B. pseudolongum Thus, B. pseudolongum, which was present at a consistently low abundance in the microbiota, but which has extensive enzymatic capacity to degrade resistant starch, showed the attributes of a keystone species associated with the bifidobacterial bloom.IMPORTANCE This study addresses the microbiology and function of a natural ecosystem (the rat gut) using DNA-based observations and in vitro experimentation.
29802187	9	60	with	analysis	1915:1922	arg1	strains					1965:1971	cultured strains	1956:1971	cultured strains of bacteria	1956:1983	Our work, combining DNA sequence analysis and laboratory experiments with cultured strains of bacteria, revealed that the increased abundance of bifidobacteria in the rat gut, induced by feeding indigestible starch, involved a species that cannot itself degrade the starch (Bifidobacterium animalis) but cohabits with a species that can (Bifidobacterium pseudolongum).
29802187	3	61	theme	present	484:490	arg1	species					476:482	the bifidobacterial species	456:482	the bifidobacterial species present in the rat cecum under these circumstances	456:533	We determined that the bifidobacterial species present in the rat cecum under these circumstances mostly belonged to Bifidobacterium animalis However, cultures of B. animalis isolated from the rats failed to degrade Hi-Maize starch to any extent.
29802187	8	62	dep	compositions	1768:1779	arg1	the					1764:1766	the	1764:1766	the	1764:1766	These studies reveal the compositions and genetic capacities of microbiotas but not the intricacies of how microbial communities function.
29802187	6	63	theme	natural	1454:1460	arg1	gut					1481:1483	the rat gut	1473:1483	the rat gut	1473:1483	Maltose was released into the culture medium, and B. animalis cultures had shorter doubling times in maltose medium than did B. pseudolongum Thus, B. pseudolongum, which was present at a consistently low abundance in the microbiota, but which has extensive enzymatic capacity to degrade resistant starch, showed the attributes of a keystone species associated with the bifidobacterial bloom.IMPORTANCE This study addresses the microbiology and function of a natural ecosystem (the rat gut) using DNA-based observations and in vitro experimentation.
29802187	6	63	theme	natural	1454:1460	arg1	ecosystem					1462:1470	a natural ecosystem	1452:1470	a natural ecosystem (the rat gut) using DNA-based observations and in vitro experimentation	1452:1542	Maltose was released into the culture medium, and B. animalis cultures had shorter doubling times in maltose medium than did B. pseudolongum Thus, B. pseudolongum, which was present at a consistently low abundance in the microbiota, but which has extensive enzymatic capacity to degrade resistant starch, showed the attributes of a keystone species associated with the bifidobacterial bloom.IMPORTANCE This study addresses the microbiology and function of a natural ecosystem (the rat gut) using DNA-based observations and in vitro experimentation.
29802187	9	64	dep	can	2215:2217	arg1	pseudolongum					2236:2247	Bifidobacterium pseudolongum	2220:2247	Bifidobacterium pseudolongum	2220:2247	Our work, combining DNA sequence analysis and laboratory experiments with cultured strains of bacteria, revealed that the increased abundance of bifidobacteria in the rat gut, induced by feeding indigestible starch, involved a species that cannot itself degrade the starch (Bifidobacterium animalis) but cohabits with a species that can (Bifidobacterium pseudolongum).
29802187	6	65	dep	in	1519:1520	arg1	vitro					1522:1526	vitro	1522:1526	vitro	1522:1526	Maltose was released into the culture medium, and B. animalis cultures had shorter doubling times in maltose medium than did B. pseudolongum Thus, B. pseudolongum, which was present at a consistently low abundance in the microbiota, but which has extensive enzymatic capacity to degrade resistant starch, showed the attributes of a keystone species associated with the bifidobacterial bloom.IMPORTANCE This study addresses the microbiology and function of a natural ecosystem (the rat gut) using DNA-based observations and in vitro experimentation.
29802187	6	66	theme	extensive	1243:1251	arg1	capacity					1263:1270	extensive enzymatic capacity	1243:1270	extensive enzymatic capacity to degrade resistant starch, showed the attributes of a keystone species associated with the bifidobacterial bloom.IMPORTANCE This study addresses the microbiology and function of a natural ecosystem (the rat gut) using DNA-based observations and in vitro experimentation	1243:1542	Maltose was released into the culture medium, and B. animalis cultures had shorter doubling times in maltose medium than did B. pseudolongum Thus, B. pseudolongum, which was present at a consistently low abundance in the microbiota, but which has extensive enzymatic capacity to degrade resistant starch, showed the attributes of a keystone species associated with the bifidobacterial bloom.IMPORTANCE This study addresses the microbiology and function of a natural ecosystem (the rat gut) using DNA-based observations and in vitro experimentation.
29802187	3	67	dep	animalis	570:577	arg1	failed					635:640	failed	635:640	failed to degrade Hi-Maize starch to any extent	635:681	We determined that the bifidobacterial species present in the rat cecum under these circumstances mostly belonged to Bifidobacterium animalis However, cultures of B. animalis isolated from the rats failed to degrade Hi-Maize starch to any extent.
29802187	6	68	dep	B.	1046:1047	arg1	animalis					1049:1056	animalis	1049:1056	animalis	1049:1056	Maltose was released into the culture medium, and B. animalis cultures had shorter doubling times in maltose medium than did B. pseudolongum Thus, B. pseudolongum, which was present at a consistently low abundance in the microbiota, but which has extensive enzymatic capacity to degrade resistant starch, showed the attributes of a keystone species associated with the bifidobacterial bloom.IMPORTANCE This study addresses the microbiology and function of a natural ecosystem (the rat gut) using DNA-based observations and in vitro experimentation.
29802187	10	69	theme	species	2305:2311	arg1	characteristics					2275:2289	the characteristics	2271:2289	the characteristics of a keystone species	2271:2311	B. pseudolongum has the characteristics of a keystone species in the community because it had low abundance but high ability to perform a critical function, the hydrolysis of resistant starch.
29802187	9	70	theme	cultured	1956:1963	arg1	strains					1965:1971	cultured strains	1956:1971	cultured strains of bacteria	1956:1983	Our work, combining DNA sequence analysis and laboratory experiments with cultured strains of bacteria, revealed that the increased abundance of bifidobacteria in the rat gut, induced by feeding indigestible starch, involved a species that cannot itself degrade the starch (Bifidobacterium animalis) but cohabits with a species that can (Bifidobacterium pseudolongum).
29802187	0	71	theme	Bifidobacterial	114:128	arg1	Blooms					130:135	Bifidobacterial Blooms	114:135	Bifidobacterial Blooms	114:135	Bifidobacterium pseudolongum in the Ceca of Rats Fed Hi-Maize Starch Has Characteristics of a Keystone Species in Bifidobacterial Blooms.
29802187	1	72	theme	mammalian	160:168	arg1	digestion					170:178	mammalian digestion	160:178	mammalian digestion	160:178	Starches resistant to mammalian digestion are present in foods and pass to the large bowel, where they may be degraded and fermented by the microbiota.
29802187	6	73	theme	DNA-based	1492:1500	arg1	observations					1502:1513	DNA-based observations	1492:1513	DNA-based observations	1492:1513	Maltose was released into the culture medium, and B. animalis cultures had shorter doubling times in maltose medium than did B. pseudolongum Thus, B. pseudolongum, which was present at a consistently low abundance in the microbiota, but which has extensive enzymatic capacity to degrade resistant starch, showed the attributes of a keystone species associated with the bifidobacterial bloom.IMPORTANCE This study addresses the microbiology and function of a natural ecosystem (the rat gut) using DNA-based observations and in vitro experimentation.
29802187	4	74	contain	had	762:764	arg1	pseudolongum					713:724	Bifidobacterium pseudolongum	697:724	Bifidobacterium pseudolongum also detected in the rat microbiota	697:760	In contrast, Bifidobacterium pseudolongum also detected in the rat microbiota had high starch-degrading ability.
29802187	4	74	contain	had	762:764	arg2	ability					788:794	high starch-degrading ability	766:794	high starch-degrading ability	766:794	In contrast, Bifidobacterium pseudolongum also detected in the rat microbiota had high starch-degrading ability.
29802187	1	75	located	present	184:190	arg2	Starches					138:145	Starches	138:145	Starches resistant to mammalian digestion	138:178	Starches resistant to mammalian digestion are present in foods and pass to the large bowel, where they may be degraded and fermented by the microbiota.
29802187	1	75	located	present	184:190	arg1	foods					195:199	foods	195:199	foods	195:199	Starches resistant to mammalian digestion are present in foods and pass to the large bowel, where they may be degraded and fermented by the microbiota.
29802187	5	76	theme	enzyme	918:923	arg1	expression					842:851	increased expression	832:851	increased expression of a type 1 pullulanase, alpha-amylase, and glycogen debranching enzyme by B. pseudolongum	832:942	Transcriptional comparisons showed increased expression of a type 1 pullulanase, alpha-amylase, and glycogen debranching enzyme by B. pseudolongum when cultured in medium containing Hi-Maize starch.
29802187	10	77	theme	high	2363:2366	arg1	ability					2368:2374	high ability	2363:2374	high ability to perform a critical function	2363:2405	B. pseudolongum has the characteristics of a keystone species in the community because it had low abundance but high ability to perform a critical function, the hydrolysis of resistant starch.
29802187	7	78	dep	sequencing	1686:1695	arg1	methods					1697:1703	methods	1697:1703	methods	1697:1703	The microbial community of the large bowel of animals, including humans, has been studied extensively through the use of high-throughput DNA sequencing methods and advanced bioinformatics analysis.
29802187	3	79	theme	animalis	603:610	arg1	cultures					588:595	cultures	588:595	cultures of B. animalis isolated from the rats	588:633	We determined that the bifidobacterial species present in the rat cecum under these circumstances mostly belonged to Bifidobacterium animalis However, cultures of B. animalis isolated from the rats failed to degrade Hi-Maize starch to any extent.
29802187	6	80	theme	low	1196:1198	arg1	abundance					1200:1208	a consistently low abundance	1181:1208	a consistently low abundance in the microbiota	1181:1226	Maltose was released into the culture medium, and B. animalis cultures had shorter doubling times in maltose medium than did B. pseudolongum Thus, B. pseudolongum, which was present at a consistently low abundance in the microbiota, but which has extensive enzymatic capacity to degrade resistant starch, showed the attributes of a keystone species associated with the bifidobacterial bloom.IMPORTANCE This study addresses the microbiology and function of a natural ecosystem (the rat gut) using DNA-based observations and in vitro experimentation.
29802187	9	81	theme	increased	2004:2012	arg1	abundance					2014:2022	the increased abundance	2000:2022	the increased abundance of bifidobacteria in the rat gut, induced by feeding indigestible starch,	2000:2096	Our work, combining DNA sequence analysis and laboratory experiments with cultured strains of bacteria, revealed that the increased abundance of bifidobacteria in the rat gut, induced by feeding indigestible starch, involved a species that cannot itself degrade the starch (Bifidobacterium animalis) but cohabits with a species that can (Bifidobacterium pseudolongum).
29802187	10	82	contain	had	2341:2343	arg2	ability					2368:2374	high ability	2363:2374	high ability to perform a critical function	2363:2405	B. pseudolongum has the characteristics of a keystone species in the community because it had low abundance but high ability to perform a critical function, the hydrolysis of resistant starch.
29802187	10	82	contain	had	2341:2343	arg2	hydrolysis					2412:2421	the hydrolysis	2408:2421	the hydrolysis of resistant starch	2408:2441	B. pseudolongum has the characteristics of a keystone species in the community because it had low abundance but high ability to perform a critical function, the hydrolysis of resistant starch.
29802187	10	82	contain	had	2341:2343	arg1	it					2338:2339	it	2338:2339	it	2338:2339	B. pseudolongum has the characteristics of a keystone species in the community because it had low abundance but high ability to perform a critical function, the hydrolysis of resistant starch.
29802187	10	82	contain	had	2341:2343	arg2	abundance					2349:2357	low abundance	2345:2357	low abundance	2345:2357	B. pseudolongum has the characteristics of a keystone species in the community because it had low abundance but high ability to perform a critical function, the hydrolysis of resistant starch.
29802187	7	83	theme	large	1576:1580	arg1	bowel					1582:1586	the large bowel	1572:1586	the large bowel of animals, including humans,	1572:1616	The microbial community of the large bowel of animals, including humans, has been studied extensively through the use of high-throughput DNA sequencing methods and advanced bioinformatics analysis.
29802187	6	84	contain	had	1067:1069	arg2	times					1088:1092	shorter doubling times	1071:1092	shorter doubling times	1071:1092	Maltose was released into the culture medium, and B. animalis cultures had shorter doubling times in maltose medium than did B. pseudolongum Thus, B. pseudolongum, which was present at a consistently low abundance in the microbiota, but which has extensive enzymatic capacity to degrade resistant starch, showed the attributes of a keystone species associated with the bifidobacterial bloom.IMPORTANCE This study addresses the microbiology and function of a natural ecosystem (the rat gut) using DNA-based observations and in vitro experimentation.
29802187	6	84	contain	had	1067:1069	arg1	cultures					1058:1065	B. animalis cultures	1046:1065	B. animalis cultures	1046:1065	Maltose was released into the culture medium, and B. animalis cultures had shorter doubling times in maltose medium than did B. pseudolongum Thus, B. pseudolongum, which was present at a consistently low abundance in the microbiota, but which has extensive enzymatic capacity to degrade resistant starch, showed the attributes of a keystone species associated with the bifidobacterial bloom.IMPORTANCE This study addresses the microbiology and function of a natural ecosystem (the rat gut) using DNA-based observations and in vitro experimentation.
29802187	3	85	from	cecum	503:507	arg1	present					484:490	present	484:490	present	484:490	We determined that the bifidobacterial species present in the rat cecum under these circumstances mostly belonged to Bifidobacterium animalis However, cultures of B. animalis isolated from the rats failed to degrade Hi-Maize starch to any extent.
29802187	6	86	theme	bifidobacterial	1365:1379	arg1	bloom.IMPORTANCE					1381:1396	the bifidobacterial bloom.IMPORTANCE	1361:1396	the bifidobacterial bloom.IMPORTANCE This study addresses the microbiology and function of a natural ecosystem (the rat gut) using DNA-based observations and in vitro experimentation	1361:1542	Maltose was released into the culture medium, and B. animalis cultures had shorter doubling times in maltose medium than did B. pseudolongum Thus, B. pseudolongum, which was present at a consistently low abundance in the microbiota, but which has extensive enzymatic capacity to degrade resistant starch, showed the attributes of a keystone species associated with the bifidobacterial bloom.IMPORTANCE This study addresses the microbiology and function of a natural ecosystem (the rat gut) using DNA-based observations and in vitro experimentation.
29802187	8	87	theme	microbiotas	1807:1817	arg1	compositions					1768:1779	compositions	1768:1779	compositions	1768:1779	These studies reveal the compositions and genetic capacities of microbiotas but not the intricacies of how microbial communities function.
29802187	8	87	theme	microbiotas	1807:1817	arg1	capacities					1793:1802	genetic capacities	1785:1802	genetic capacities	1785:1802	These studies reveal the compositions and genetic capacities of microbiotas but not the intricacies of how microbial communities function.
29802187	5	88	theme	glycogen	897:904	arg1	enzyme					918:923	glycogen debranching enzyme	897:923	glycogen debranching enzyme	897:923	Transcriptional comparisons showed increased expression of a type 1 pullulanase, alpha-amylase, and glycogen debranching enzyme by B. pseudolongum when cultured in medium containing Hi-Maize starch.
29802187	7	89	theme	analysis	1733:1740	arg1	use					1659:1661	the use	1655:1661	the use of high-throughput DNA sequencing methods and advanced bioinformatics analysis	1655:1740	The microbial community of the large bowel of animals, including humans, has been studied extensively through the use of high-throughput DNA sequencing methods and advanced bioinformatics analysis.
29802187	0	90	from	pseudolongum	16:27	arg1	Ceca					36:39	the Ceca	32:39	the Ceca of Rats Fed Hi-Maize Starch	32:67	Bifidobacterium pseudolongum in the Ceca of Rats Fed Hi-Maize Starch Has Characteristics of a Keystone Species in Bifidobacterial Blooms.
29802187	6	91	from	abundance	1200:1208	arg1	present					1170:1176	present	1170:1176	present	1170:1176	Maltose was released into the culture medium, and B. animalis cultures had shorter doubling times in maltose medium than did B. pseudolongum Thus, B. pseudolongum, which was present at a consistently low abundance in the microbiota, but which has extensive enzymatic capacity to degrade resistant starch, showed the attributes of a keystone species associated with the bifidobacterial bloom.IMPORTANCE This study addresses the microbiology and function of a natural ecosystem (the rat gut) using DNA-based observations and in vitro experimentation.
29802187	6	91	from	abundance	1200:1208	arg1	microbiota					1217:1226	the microbiota	1213:1226	the microbiota	1213:1226	Maltose was released into the culture medium, and B. animalis cultures had shorter doubling times in maltose medium than did B. pseudolongum Thus, B. pseudolongum, which was present at a consistently low abundance in the microbiota, but which has extensive enzymatic capacity to degrade resistant starch, showed the attributes of a keystone species associated with the bifidobacterial bloom.IMPORTANCE This study addresses the microbiology and function of a natural ecosystem (the rat gut) using DNA-based observations and in vitro experimentation.
29802187	6	92	dep	did	1117:1119	arg1	has					1239:1241	has	1239:1241	has extensive enzymatic capacity to degrade resistant starch, showed the attributes of a keystone species associated with the bifidobacterial bloom.IMPORTANCE This study addresses the microbiology and function of a natural ecosystem (the rat gut) using DNA-based observations and in vitro experimentation	1239:1542	Maltose was released into the culture medium, and B. animalis cultures had shorter doubling times in maltose medium than did B. pseudolongum Thus, B. pseudolongum, which was present at a consistently low abundance in the microbiota, but which has extensive enzymatic capacity to degrade resistant starch, showed the attributes of a keystone species associated with the bifidobacterial bloom.IMPORTANCE This study addresses the microbiology and function of a natural ecosystem (the rat gut) using DNA-based observations and in vitro experimentation.
29802187	6	92	dep	did	1117:1119	arg1	present					1170:1176	present	1170:1176	present	1170:1176	Maltose was released into the culture medium, and B. animalis cultures had shorter doubling times in maltose medium than did B. pseudolongum Thus, B. pseudolongum, which was present at a consistently low abundance in the microbiota, but which has extensive enzymatic capacity to degrade resistant starch, showed the attributes of a keystone species associated with the bifidobacterial bloom.IMPORTANCE This study addresses the microbiology and function of a natural ecosystem (the rat gut) using DNA-based observations and in vitro experimentation.
29802187	7	93	theme	advanced	1709:1716	arg1	analysis					1733:1740	advanced bioinformatics analysis	1709:1740	advanced bioinformatics analysis	1709:1740	The microbial community of the large bowel of animals, including humans, has been studied extensively through the use of high-throughput DNA sequencing methods and advanced bioinformatics analysis.
29802187	7	94	theme	microbial	1549:1557	arg1	community					1559:1567	The microbial community	1545:1567	The microbial community of the large bowel of animals, including humans,	1545:1616	The microbial community of the large bowel of animals, including humans, has been studied extensively through the use of high-throughput DNA sequencing methods and advanced bioinformatics analysis.
29802187	9	95	theme	rat	2049:2051	arg1	gut					2053:2055	the rat gut	2045:2055	the rat gut	2045:2055	Our work, combining DNA sequence analysis and laboratory experiments with cultured strains of bacteria, revealed that the increased abundance of bifidobacteria in the rat gut, induced by feeding indigestible starch, involved a species that cannot itself degrade the starch (Bifidobacterium animalis) but cohabits with a species that can (Bifidobacterium pseudolongum).
29802187	0	96	theme	Species	103:109	arg1	Characteristics					73:87	Characteristics	73:87	Characteristics of a Keystone Species	73:109	Bifidobacterium pseudolongum in the Ceca of Rats Fed Hi-Maize Starch Has Characteristics of a Keystone Species in Bifidobacterial Blooms.
29802187	6	97	theme	species	1337:1343	arg1	attributes					1312:1321	the attributes	1308:1321	the attributes of a keystone species associated with the bifidobacterial bloom.IMPORTANCE This study addresses the microbiology and function of a natural ecosystem (the rat gut) using DNA-based observations and in vitro experimentation	1308:1542	Maltose was released into the culture medium, and B. animalis cultures had shorter doubling times in maltose medium than did B. pseudolongum Thus, B. pseudolongum, which was present at a consistently low abundance in the microbiota, but which has extensive enzymatic capacity to degrade resistant starch, showed the attributes of a keystone species associated with the bifidobacterial bloom.IMPORTANCE This study addresses the microbiology and function of a natural ecosystem (the rat gut) using DNA-based observations and in vitro experimentation.
29802187	5	98	theme	Transcriptional	797:811	arg1	comparisons					813:823	Transcriptional comparisons	797:823	Transcriptional comparisons	797:823	Transcriptional comparisons showed increased expression of a type 1 pullulanase, alpha-amylase, and glycogen debranching enzyme by B. pseudolongum when cultured in medium containing Hi-Maize starch.
29802187	7	99	theme	DNA	1682:1684	arg1	sequencing					1686:1695	high-throughput DNA sequencing	1666:1695	high-throughput DNA sequencing	1666:1695	The microbial community of the large bowel of animals, including humans, has been studied extensively through the use of high-throughput DNA sequencing methods and advanced bioinformatics analysis.
29802187	6	100	theme	shorter	1071:1077	arg1	times					1088:1092	shorter doubling times	1071:1092	shorter doubling times	1071:1092	Maltose was released into the culture medium, and B. animalis cultures had shorter doubling times in maltose medium than did B. pseudolongum Thus, B. pseudolongum, which was present at a consistently low abundance in the microbiota, but which has extensive enzymatic capacity to degrade resistant starch, showed the attributes of a keystone species associated with the bifidobacterial bloom.IMPORTANCE This study addresses the microbiology and function of a natural ecosystem (the rat gut) using DNA-based observations and in vitro experimentation.
29802187	2	101	theme	Hi-Maize	410:417	arg1	starch					429:434	Hi-Maize resistant starch	410:434	Hi-Maize resistant starch	410:434	Increases in relative abundances of bifidobacteria (blooms) have been reported in rats whose diet was supplemented with Hi-Maize resistant starch.
29802187	9	102	theme	indigestible	2077:2088	arg1	starch					2090:2095	indigestible starch	2077:2095	indigestible starch	2077:2095	Our work, combining DNA sequence analysis and laboratory experiments with cultured strains of bacteria, revealed that the increased abundance of bifidobacteria in the rat gut, induced by feeding indigestible starch, involved a species that cannot itself degrade the starch (Bifidobacterium animalis) but cohabits with a species that can (Bifidobacterium pseudolongum).
29802187	6	103	theme	B.	1046:1047	arg1	cultures					1058:1065	B. animalis cultures	1046:1065	B. animalis cultures	1046:1065	Maltose was released into the culture medium, and B. animalis cultures had shorter doubling times in maltose medium than did B. pseudolongum Thus, B. pseudolongum, which was present at a consistently low abundance in the microbiota, but which has extensive enzymatic capacity to degrade resistant starch, showed the attributes of a keystone species associated with the bifidobacterial bloom.IMPORTANCE This study addresses the microbiology and function of a natural ecosystem (the rat gut) using DNA-based observations and in vitro experimentation.
29802187	0	104	theme	Keystone	94:101	arg1	Species					103:109	a Keystone Species	92:109	a Keystone Species	92:109	Bifidobacterium pseudolongum in the Ceca of Rats Fed Hi-Maize Starch Has Characteristics of a Keystone Species in Bifidobacterial Blooms.
29802187	4	105	located	detected	731:738	arg2	pseudolongum					713:724	Bifidobacterium pseudolongum	697:724	Bifidobacterium pseudolongum also detected in the rat microbiota	697:760	In contrast, Bifidobacterium pseudolongum also detected in the rat microbiota had high starch-degrading ability.
29802187	4	105	located	detected	731:738	arg1	microbiota					751:760	the rat microbiota	743:760	the rat microbiota	743:760	In contrast, Bifidobacterium pseudolongum also detected in the rat microbiota had high starch-degrading ability.
29802187	9	106	with	experiments	1939:1949	arg1	strains					1965:1971	cultured strains	1956:1971	cultured strains of bacteria	1956:1983	Our work, combining DNA sequence analysis and laboratory experiments with cultured strains of bacteria, revealed that the increased abundance of bifidobacteria in the rat gut, induced by feeding indigestible starch, involved a species that cannot itself degrade the starch (Bifidobacterium animalis) but cohabits with a species that can (Bifidobacterium pseudolongum).
29802187	3	107	attach	present	484:490	arg1	cecum					503:507	the rat cecum	495:507	the rat cecum under these circumstances	495:533	We determined that the bifidobacterial species present in the rat cecum under these circumstances mostly belonged to Bifidobacterium animalis However, cultures of B. animalis isolated from the rats failed to degrade Hi-Maize starch to any extent.
29802187	3	107	attach	present	484:490	arg2	species					476:482	the bifidobacterial species	456:482	the bifidobacterial species present in the rat cecum under these circumstances	456:533	We determined that the bifidobacterial species present in the rat cecum under these circumstances mostly belonged to Bifidobacterium animalis However, cultures of B. animalis isolated from the rats failed to degrade Hi-Maize starch to any extent.
29802187	4	108	theme	high	766:769	arg1	ability					788:794	high starch-degrading ability	766:794	high starch-degrading ability	766:794	In contrast, Bifidobacterium pseudolongum also detected in the rat microbiota had high starch-degrading ability.
29802187	0	109	contain	Has	69:71	arg2	Characteristics					73:87	Characteristics	73:87	Characteristics of a Keystone Species	73:109	Bifidobacterium pseudolongum in the Ceca of Rats Fed Hi-Maize Starch Has Characteristics of a Keystone Species in Bifidobacterial Blooms.
29802187	0	109	contain	Has	69:71	arg1	pseudolongum					16:27	Bifidobacterium pseudolongum	0:27	Bifidobacterium pseudolongum in the Ceca of Rats Fed Hi-Maize Starch	0:67	Bifidobacterium pseudolongum in the Ceca of Rats Fed Hi-Maize Starch Has Characteristics of a Keystone Species in Bifidobacterial Blooms.
29802187	1	110	theme	large	217:221	arg1	bowel					223:227	the large bowel	213:227	the large bowel	213:227	Starches resistant to mammalian digestion are present in foods and pass to the large bowel, where they may be degraded and fermented by the microbiota.
29802187	3	111	theme	rat	499:501	arg1	cecum					503:507	the rat cecum	495:507	the rat cecum under these circumstances	495:533	We determined that the bifidobacterial species present in the rat cecum under these circumstances mostly belonged to Bifidobacterium animalis However, cultures of B. animalis isolated from the rats failed to degrade Hi-Maize starch to any extent.
31300470	10	0	theme	response	1891:1898	arg1	part					1873:1876	part	1873:1876	part of the plant response to osmotic stress	1873:1916	Together, the data indicate that reorganization of receptor-like-kinases to plasmodesmata is important for the regulation of callose and LR development as part of the plant response to osmotic stress.
31300470	4	1	theme	PM-associated	863:875	arg1	proteins					877:884	PM-associated proteins	863:884	PM-associated proteins	863:884	This process is remarkably fast, is not a general feature of PM-associated proteins, and is independent of sterol and sphingolipid membrane composition.
31300470	10	2	theme	osmotic	1903:1909	arg1	stress					1911:1916	osmotic stress	1903:1916	osmotic stress	1903:1916	Together, the data indicate that reorganization of receptor-like-kinases to plasmodesmata is important for the regulation of callose and LR development as part of the plant response to osmotic stress.
31300470	2	3	theme	plasma	368:373	arg1	PM					385:386	PM	385:386	PM	385:386	While many of the developmental, biotic, and abiotic signals are primarily perceived at the plasma membrane (PM) by receptor proteins, plasmodesmata also cluster receptor-like activities; whether these two pathways interact is currently unknown.
31300470	2	3	theme	plasma	368:373	arg1	membrane					375:382	the plasma membrane	364:382	the plasma membrane (PM)	364:387	While many of the developmental, biotic, and abiotic signals are primarily perceived at the plasma membrane (PM) by receptor proteins, plasmodesmata also cluster receptor-like activities; whether these two pathways interact is currently unknown.
31300470	0	4	theme	Osmotic	92:98	arg1	Stress					100:105	Osmotic Stress	92:105	Osmotic Stress	92:105	Plasma Membrane-Associated Receptor-like Kinases Relocalize to Plasmodesmata in Response to Osmotic Stress.
31300470	10	5	theme	plant	1885:1889	arg1	response					1891:1898	the plant response	1881:1898	the plant response to osmotic stress	1881:1916	Together, the data indicate that reorganization of receptor-like-kinases to plasmodesmata is important for the regulation of callose and LR development as part of the plant response to osmotic stress.
31300470	9	6	theme	QSK1	1661:1664	arg1	mutant					1666:1671	phosphodead QSK1 mutant	1649:1671	phosphodead QSK1 mutant	1649:1671	Both the LR and callose phenotypes can be complemented by expression of wild-type and phosphomimic QSK1 variants, but not by phosphodead QSK1 mutant, which fails to relocalize at plasmodesmata.
31300470	6	7	theme	mannitol	1249:1256	arg1	stress					1258:1263	mannitol stress	1249:1263	mannitol stress	1249:1263	Loss-of-function mutation in QSK1 results in delayed lateral root (LR) development, and the mutant is affected in the root response to mannitol stress.
31300470	9	8	theme	variants	1628:1635	arg1	expression					1582:1591	expression	1582:1591	expression of wild-type and phosphomimic QSK1 variants	1582:1635	Both the LR and callose phenotypes can be complemented by expression of wild-type and phosphomimic QSK1 variants, but not by phosphodead QSK1 mutant, which fails to relocalize at plasmodesmata.
31300470	7	9	theme	LR	1329:1330	arg1	development					1332:1342	LR development	1329:1342	LR development	1329:1342	Callose-mediated plasmodesmata regulation is known to regulate LR development.
31300470	8	10	theme	mutant	1398:1403	arg1	background					1405:1414	the qsk1 mutant background	1389:1414	the qsk1 mutant background	1389:1414	We found that callose levels are reduced in the qsk1 mutant background with a root phenotype resembling ectopic expression of PdBG1, an enzyme that degrades callose at the pores.
31300470	2	11	theme	biotic	309:314	arg1	signals					329:335	the developmental, biotic, and abiotic signals	290:335	the developmental, biotic, and abiotic signals	290:335	While many of the developmental, biotic, and abiotic signals are primarily perceived at the plasma membrane (PM) by receptor proteins, plasmodesmata also cluster receptor-like activities; whether these two pathways interact is currently unknown.
31300470	9	12	theme	LR	1533:1534	arg1	phenotypes					1548:1557	Both the LR and callose phenotypes	1524:1557	phenotypes	1548:1557	Both the LR and callose phenotypes can be complemented by expression of wild-type and phosphomimic QSK1 variants, but not by phosphodead QSK1 mutant, which fails to relocalize at plasmodesmata.
31300470	4	13	theme	composition	942:952	arg1	independent					894:904	independent	894:904	independent	894:904	This process is remarkably fast, is not a general feature of PM-associated proteins, and is independent of sterol and sphingolipid membrane composition.
31300470	4	14	theme	proteins	877:884	arg1	feature					852:858	a general feature	842:858	a general feature of PM-associated proteins	842:884	This process is remarkably fast, is not a general feature of PM-associated proteins, and is independent of sterol and sphingolipid membrane composition.
31300470	3	15	theme	PM-located	550:559	arg1	receptor-like-kinases					577:597	specific PM-located Leu-rich-repeat receptor-like-kinases	541:597	specific PM-located Leu-rich-repeat receptor-like-kinases	541:597	Here, we show that specific PM-located Leu-rich-repeat receptor-like-kinases, Qiān Shŏu kinase (QSK1) and inflorescence meristem kinase2, which under optimal growth conditions are absent from plasmodesmata, rapidly relocate and cluster to the pores in response to osmotic stress.
31300470	3	15	theme	PM-located	550:559	arg1	kinase2					651:657	inflorescence meristem kinase2	628:657	inflorescence meristem kinase2	628:657	Here, we show that specific PM-located Leu-rich-repeat receptor-like-kinases, Qiān Shŏu kinase (QSK1) and inflorescence meristem kinase2, which under optimal growth conditions are absent from plasmodesmata, rapidly relocate and cluster to the pores in response to osmotic stress.
31300470	3	15	theme	PM-located	550:559	arg1	kinase					610:615	Qiān Shŏu kinase	600:615	Qiān Shŏu kinase (QSK1)	600:622	Here, we show that specific PM-located Leu-rich-repeat receptor-like-kinases, Qiān Shŏu kinase (QSK1) and inflorescence meristem kinase2, which under optimal growth conditions are absent from plasmodesmata, rapidly relocate and cluster to the pores in response to osmotic stress.
31300470	6	16	theme	Loss-of-function	1114:1129	arg1	mutation					1131:1138	Loss-of-function mutation	1114:1138	Loss-of-function mutation in QSK1	1114:1146	Loss-of-function mutation in QSK1 results in delayed lateral root (LR) development, and the mutant is affected in the root response to mannitol stress.
31300470	5	17	theme	QSK1	1092:1095	arg1	phosphorylation					1097:1111	QSK1 phosphorylation	1092:1111	QSK1 phosphorylation	1092:1111	Focusing on QSK1, previously reported to be involved in stress responses, we show that relocalization in response to mannitol depends on QSK1 phosphorylation.
31300470	2	18	theme	developmental	294:306	arg1	signals					329:335	the developmental, biotic, and abiotic signals	290:335	the developmental, biotic, and abiotic signals	290:335	While many of the developmental, biotic, and abiotic signals are primarily perceived at the plasma membrane (PM) by receptor proteins, plasmodesmata also cluster receptor-like activities; whether these two pathways interact is currently unknown.
31300470	1	19	theme	related	197:203	arg1	processes					187:195	processes	187:195	processes related to plant growth, development, and responses to environmental stresses	187:273	Plasmodesmata act as key elements in intercellular communication, coordinating processes related to plant growth, development, and responses to environmental stresses.
31300470	9	20	theme	phosphodead	1649:1659	arg1	mutant					1666:1671	phosphodead QSK1 mutant	1649:1671	phosphodead QSK1 mutant	1649:1671	Both the LR and callose phenotypes can be complemented by expression of wild-type and phosphomimic QSK1 variants, but not by phosphodead QSK1 mutant, which fails to relocalize at plasmodesmata.
31300470	6	21	theme	root	1175:1178	arg1	development					1185:1195	delayed lateral root (LR) development	1159:1195	delayed lateral root (LR) development	1159:1195	Loss-of-function mutation in QSK1 results in delayed lateral root (LR) development, and the mutant is affected in the root response to mannitol stress.
31300470	0	22	theme	Membrane-Associated	7:25	arg1	Kinases					41:47	Plasma Membrane-Associated Receptor-like Kinases	0:47	Plasma Membrane-Associated Receptor-like Kinases	0:47	Plasma Membrane-Associated Receptor-like Kinases Relocalize to Plasmodesmata in Response to Osmotic Stress.
31300470	6	23	theme	lateral	1167:1173	arg1	development					1185:1195	delayed lateral root (LR) development	1159:1195	delayed lateral root (LR) development	1159:1195	Loss-of-function mutation in QSK1 results in delayed lateral root (LR) development, and the mutant is affected in the root response to mannitol stress.
31300470	6	24	theme	root	1232:1235	arg1	response					1237:1244	the root response	1228:1244	the root response to mannitol stress	1228:1263	Loss-of-function mutation in QSK1 results in delayed lateral root (LR) development, and the mutant is affected in the root response to mannitol stress.
31300470	2	25	theme	signals	329:335	arg1	many					282:285	many	282:285	many	282:285	While many of the developmental, biotic, and abiotic signals are primarily perceived at the plasma membrane (PM) by receptor proteins, plasmodesmata also cluster receptor-like activities; whether these two pathways interact is currently unknown.
31300470	2	25	theme	signals	329:335	arg1	signals					329:335	the developmental, biotic, and abiotic signals	290:335	the developmental, biotic, and abiotic signals	290:335	While many of the developmental, biotic, and abiotic signals are primarily perceived at the plasma membrane (PM) by receptor proteins, plasmodesmata also cluster receptor-like activities; whether these two pathways interact is currently unknown.
31300470	1	26	theme	plant	208:212	arg1	growth					214:219	plant growth	208:219	plant growth	208:219	Plasmodesmata act as key elements in intercellular communication, coordinating processes related to plant growth, development, and responses to environmental stresses.
31300470	0	27	theme	Plasma	0:5	arg1	Kinases					41:47	Plasma Membrane-Associated Receptor-like Kinases	0:47	Plasma Membrane-Associated Receptor-like Kinases	0:47	Plasma Membrane-Associated Receptor-like Kinases Relocalize to Plasmodesmata in Response to Osmotic Stress.
31300470	4	28	theme	general	844:850	arg1	feature					852:858	a general feature	842:858	a general feature of PM-associated proteins	842:884	This process is remarkably fast, is not a general feature of PM-associated proteins, and is independent of sterol and sphingolipid membrane composition.
31300470	2	29	theme	abiotic	321:327	arg1	signals					329:335	the developmental, biotic, and abiotic signals	290:335	the developmental, biotic, and abiotic signals	290:335	While many of the developmental, biotic, and abiotic signals are primarily perceived at the plasma membrane (PM) by receptor proteins, plasmodesmata also cluster receptor-like activities; whether these two pathways interact is currently unknown.
31300470	2	30	theme	receptor-like	438:450	arg1	activities					452:461	receptor-like activities	438:461	receptor-like activities	438:461	While many of the developmental, biotic, and abiotic signals are primarily perceived at the plasma membrane (PM) by receptor proteins, plasmodesmata also cluster receptor-like activities; whether these two pathways interact is currently unknown.
31300470	9	31	theme	phosphomimic	1610:1621	arg1	variants					1628:1635	wild-type and phosphomimic QSK1 variants	1596:1635	wild-type and phosphomimic QSK1 variants	1596:1635	Both the LR and callose phenotypes can be complemented by expression of wild-type and phosphomimic QSK1 variants, but not by phosphodead QSK1 mutant, which fails to relocalize at plasmodesmata.
31300470	1	32	from	elements	133:140	arg1	communication					159:171	intercellular communication	145:171	intercellular communication	145:171	Plasmodesmata act as key elements in intercellular communication, coordinating processes related to plant growth, development, and responses to environmental stresses.
31300470	3	33	theme	Leu-rich-repeat	561:575	arg1	receptor-like-kinases					577:597	specific PM-located Leu-rich-repeat receptor-like-kinases	541:597	specific PM-located Leu-rich-repeat receptor-like-kinases	541:597	Here, we show that specific PM-located Leu-rich-repeat receptor-like-kinases, Qiān Shŏu kinase (QSK1) and inflorescence meristem kinase2, which under optimal growth conditions are absent from plasmodesmata, rapidly relocate and cluster to the pores in response to osmotic stress.
31300470	3	33	theme	Leu-rich-repeat	561:575	arg1	kinase2					651:657	inflorescence meristem kinase2	628:657	inflorescence meristem kinase2	628:657	Here, we show that specific PM-located Leu-rich-repeat receptor-like-kinases, Qiān Shŏu kinase (QSK1) and inflorescence meristem kinase2, which under optimal growth conditions are absent from plasmodesmata, rapidly relocate and cluster to the pores in response to osmotic stress.
31300470	3	33	theme	Leu-rich-repeat	561:575	arg1	kinase					610:615	Qiān Shŏu kinase	600:615	Qiān Shŏu kinase (QSK1)	600:622	Here, we show that specific PM-located Leu-rich-repeat receptor-like-kinases, Qiān Shŏu kinase (QSK1) and inflorescence meristem kinase2, which under optimal growth conditions are absent from plasmodesmata, rapidly relocate and cluster to the pores in response to osmotic stress.
31300470	0	34	theme	Receptor-like	27:39	arg1	Kinases					41:47	Plasma Membrane-Associated Receptor-like Kinases	0:47	Plasma Membrane-Associated Receptor-like Kinases	0:47	Plasma Membrane-Associated Receptor-like Kinases Relocalize to Plasmodesmata in Response to Osmotic Stress.
31300470	10	35	theme	LR	1855:1856	arg1	development					1858:1868	LR development	1855:1868	LR development	1855:1868	Together, the data indicate that reorganization of receptor-like-kinases to plasmodesmata is important for the regulation of callose and LR development as part of the plant response to osmotic stress.
31300470	4	36	theme	sterol	909:914	arg1	independent					894:904	independent	894:904	independent	894:904	This process is remarkably fast, is not a general feature of PM-associated proteins, and is independent of sterol and sphingolipid membrane composition.
31300470	8	37	from	pores	1517:1521	arg1	callose					1502:1508	callose	1502:1508	callose at the pores	1502:1521	We found that callose levels are reduced in the qsk1 mutant background with a root phenotype resembling ectopic expression of PdBG1, an enzyme that degrades callose at the pores.
31300470	9	38	dep	phenotypes	1548:1557	arg1	Both					1524:1527	Both	1524:1527	Both	1524:1527	Both the LR and callose phenotypes can be complemented by expression of wild-type and phosphomimic QSK1 variants, but not by phosphodead QSK1 mutant, which fails to relocalize at plasmodesmata.
31300470	6	39	theme	delayed	1159:1165	arg1	development					1185:1195	delayed lateral root (LR) development	1159:1195	delayed lateral root (LR) development	1159:1195	Loss-of-function mutation in QSK1 results in delayed lateral root (LR) development, and the mutant is affected in the root response to mannitol stress.
31300470	9	40	theme	callose	1540:1546	arg1	phenotypes					1548:1557	Both the LR and callose phenotypes	1524:1557	phenotypes	1548:1557	Both the LR and callose phenotypes can be complemented by expression of wild-type and phosphomimic QSK1 variants, but not by phosphodead QSK1 mutant, which fails to relocalize at plasmodesmata.
31300470	4	41	theme	membrane	933:940	arg1	composition					942:952	sphingolipid membrane composition	920:952	sphingolipid membrane composition	920:952	This process is remarkably fast, is not a general feature of PM-associated proteins, and is independent of sterol and sphingolipid membrane composition.
31300470	5	42	theme	stress	1011:1016	arg1	responses					1018:1026	stress responses	1011:1026	stress responses	1011:1026	Focusing on QSK1, previously reported to be involved in stress responses, we show that relocalization in response to mannitol depends on QSK1 phosphorylation.
31300470	7	43	theme	plasmodesmata	1283:1295	arg1	regulation					1297:1306	Callose-mediated plasmodesmata regulation	1266:1306	Callose-mediated plasmodesmata regulation	1266:1306	Callose-mediated plasmodesmata regulation is known to regulate LR development.
31300470	2	44	theme	receptor	392:399	arg1	proteins					401:408	receptor proteins	392:408	receptor proteins	392:408	While many of the developmental, biotic, and abiotic signals are primarily perceived at the plasma membrane (PM) by receptor proteins, plasmodesmata also cluster receptor-like activities; whether these two pathways interact is currently unknown.
31300470	3	45	theme	osmotic	786:792	arg1	stress					794:799	osmotic stress	786:799	osmotic stress	786:799	Here, we show that specific PM-located Leu-rich-repeat receptor-like-kinases, Qiān Shŏu kinase (QSK1) and inflorescence meristem kinase2, which under optimal growth conditions are absent from plasmodesmata, rapidly relocate and cluster to the pores in response to osmotic stress.
31300470	4	46	theme	sphingolipid	920:931	arg1	composition					942:952	sphingolipid membrane composition	920:952	sphingolipid membrane composition	920:952	This process is remarkably fast, is not a general feature of PM-associated proteins, and is independent of sterol and sphingolipid membrane composition.
31300470	10	47	theme	callose	1843:1849	arg1	regulation					1829:1838	the regulation	1825:1838	the regulation of callose and LR development	1825:1868	Together, the data indicate that reorganization of receptor-like-kinases to plasmodesmata is important for the regulation of callose and LR development as part of the plant response to osmotic stress.
31300470	6	48	from	mutation	1131:1138	arg1	QSK1					1143:1146	QSK1	1143:1146	QSK1	1143:1146	Loss-of-function mutation in QSK1 results in delayed lateral root (LR) development, and the mutant is affected in the root response to mannitol stress.
31300470	3	49	from	plasmodesmata	714:726	arg1	absent					702:707	absent	702:707	absent	702:707	Here, we show that specific PM-located Leu-rich-repeat receptor-like-kinases, Qiān Shŏu kinase (QSK1) and inflorescence meristem kinase2, which under optimal growth conditions are absent from plasmodesmata, rapidly relocate and cluster to the pores in response to osmotic stress.
31300470	8	50	theme	ectopic	1449:1455	arg1	expression					1457:1466	ectopic expression	1449:1466	ectopic expression of PdBG1, an enzyme that degrades callose at the pores	1449:1521	We found that callose levels are reduced in the qsk1 mutant background with a root phenotype resembling ectopic expression of PdBG1, an enzyme that degrades callose at the pores.
31300470	3	51	theme	Shŏu	605:608	arg1	receptor-like-kinases					577:597	specific PM-located Leu-rich-repeat receptor-like-kinases	541:597	specific PM-located Leu-rich-repeat receptor-like-kinases	541:597	Here, we show that specific PM-located Leu-rich-repeat receptor-like-kinases, Qiān Shŏu kinase (QSK1) and inflorescence meristem kinase2, which under optimal growth conditions are absent from plasmodesmata, rapidly relocate and cluster to the pores in response to osmotic stress.
31300470	3	51	theme	Shŏu	605:608	arg1	QSK1					618:621	QSK1	618:621	QSK1	618:621	Here, we show that specific PM-located Leu-rich-repeat receptor-like-kinases, Qiān Shŏu kinase (QSK1) and inflorescence meristem kinase2, which under optimal growth conditions are absent from plasmodesmata, rapidly relocate and cluster to the pores in response to osmotic stress.
31300470	3	51	theme	Shŏu	605:608	arg1	kinase					610:615	Qiān Shŏu kinase	600:615	Qiān Shŏu kinase (QSK1)	600:622	Here, we show that specific PM-located Leu-rich-repeat receptor-like-kinases, Qiān Shŏu kinase (QSK1) and inflorescence meristem kinase2, which under optimal growth conditions are absent from plasmodesmata, rapidly relocate and cluster to the pores in response to osmotic stress.
31300470	8	52	theme	callose	1359:1365	arg1	levels					1367:1372	callose levels	1359:1372	callose levels	1359:1372	We found that callose levels are reduced in the qsk1 mutant background with a root phenotype resembling ectopic expression of PdBG1, an enzyme that degrades callose at the pores.
31300470	1	53	theme	key	129:131	arg1	Plasmodesmata					108:120	Plasmodesmata	108:120	Plasmodesmata	108:120	Plasmodesmata act as key elements in intercellular communication, coordinating processes related to plant growth, development, and responses to environmental stresses.
31300470	1	53	theme	key	129:131	arg1	elements					133:140	key elements	129:140	key elements in intercellular communication	129:171	Plasmodesmata act as key elements in intercellular communication, coordinating processes related to plant growth, development, and responses to environmental stresses.
31300470	6	54	theme	LR	1181:1182	arg1	development					1185:1195	delayed lateral root (LR) development	1159:1195	delayed lateral root (LR) development	1159:1195	Loss-of-function mutation in QSK1 results in delayed lateral root (LR) development, and the mutant is affected in the root response to mannitol stress.
31300470	8	55	theme	PdBG1	1471:1475	arg1	expression					1457:1466	ectopic expression	1449:1466	ectopic expression of PdBG1, an enzyme that degrades callose at the pores	1449:1521	We found that callose levels are reduced in the qsk1 mutant background with a root phenotype resembling ectopic expression of PdBG1, an enzyme that degrades callose at the pores.
31300470	8	56	theme	qsk1	1393:1396	arg1	background					1405:1414	the qsk1 mutant background	1389:1414	the qsk1 mutant background	1389:1414	We found that callose levels are reduced in the qsk1 mutant background with a root phenotype resembling ectopic expression of PdBG1, an enzyme that degrades callose at the pores.
31300470	7	57	theme	Callose-mediated	1266:1281	arg1	regulation					1297:1306	Callose-mediated plasmodesmata regulation	1266:1306	Callose-mediated plasmodesmata regulation	1266:1306	Callose-mediated plasmodesmata regulation is known to regulate LR development.
31300470	10	58	theme	receptor-like-kinases	1769:1789	arg1	reorganization					1751:1764	reorganization	1751:1764	reorganization of receptor-like-kinases to plasmodesmata	1751:1806	Together, the data indicate that reorganization of receptor-like-kinases to plasmodesmata is important for the regulation of callose and LR development as part of the plant response to osmotic stress.
31300470	9	59	theme	wild-type	1596:1604	arg1	variants					1628:1635	wild-type and phosphomimic QSK1 variants	1596:1635	wild-type and phosphomimic QSK1 variants	1596:1635	Both the LR and callose phenotypes can be complemented by expression of wild-type and phosphomimic QSK1 variants, but not by phosphodead QSK1 mutant, which fails to relocalize at plasmodesmata.
31300470	3	60	theme	inflorescence	628:640	arg1	receptor-like-kinases					577:597	specific PM-located Leu-rich-repeat receptor-like-kinases	541:597	specific PM-located Leu-rich-repeat receptor-like-kinases	541:597	Here, we show that specific PM-located Leu-rich-repeat receptor-like-kinases, Qiān Shŏu kinase (QSK1) and inflorescence meristem kinase2, which under optimal growth conditions are absent from plasmodesmata, rapidly relocate and cluster to the pores in response to osmotic stress.
31300470	3	60	theme	inflorescence	628:640	arg1	kinase2					651:657	inflorescence meristem kinase2	628:657	inflorescence meristem kinase2	628:657	Here, we show that specific PM-located Leu-rich-repeat receptor-like-kinases, Qiān Shŏu kinase (QSK1) and inflorescence meristem kinase2, which under optimal growth conditions are absent from plasmodesmata, rapidly relocate and cluster to the pores in response to osmotic stress.
31300470	3	61	theme	optimal	672:678	arg1	conditions					687:696	optimal growth conditions	672:696	optimal growth conditions	672:696	Here, we show that specific PM-located Leu-rich-repeat receptor-like-kinases, Qiān Shŏu kinase (QSK1) and inflorescence meristem kinase2, which under optimal growth conditions are absent from plasmodesmata, rapidly relocate and cluster to the pores in response to osmotic stress.
31300470	3	62	theme	meristem	642:649	arg1	receptor-like-kinases					577:597	specific PM-located Leu-rich-repeat receptor-like-kinases	541:597	specific PM-located Leu-rich-repeat receptor-like-kinases	541:597	Here, we show that specific PM-located Leu-rich-repeat receptor-like-kinases, Qiān Shŏu kinase (QSK1) and inflorescence meristem kinase2, which under optimal growth conditions are absent from plasmodesmata, rapidly relocate and cluster to the pores in response to osmotic stress.
31300470	3	62	theme	meristem	642:649	arg1	kinase2					651:657	inflorescence meristem kinase2	628:657	inflorescence meristem kinase2	628:657	Here, we show that specific PM-located Leu-rich-repeat receptor-like-kinases, Qiān Shŏu kinase (QSK1) and inflorescence meristem kinase2, which under optimal growth conditions are absent from plasmodesmata, rapidly relocate and cluster to the pores in response to osmotic stress.
31300470	1	63	theme	intercellular	145:157	arg1	communication					159:171	intercellular communication	145:171	intercellular communication	145:171	Plasmodesmata act as key elements in intercellular communication, coordinating processes related to plant growth, development, and responses to environmental stresses.
31300470	5	64	from	relocalization	1042:1055	arg1	response					1060:1067	response	1060:1067	response to mannitol	1060:1079	Focusing on QSK1, previously reported to be involved in stress responses, we show that relocalization in response to mannitol depends on QSK1 phosphorylation.
31300470	3	65	theme	growth	680:685	arg1	conditions					687:696	optimal growth conditions	672:696	optimal growth conditions	672:696	Here, we show that specific PM-located Leu-rich-repeat receptor-like-kinases, Qiān Shŏu kinase (QSK1) and inflorescence meristem kinase2, which under optimal growth conditions are absent from plasmodesmata, rapidly relocate and cluster to the pores in response to osmotic stress.
31300470	8	66	theme	root	1423:1426	arg1	phenotype					1428:1436	a root phenotype	1421:1436	a root phenotype resembling ectopic expression of PdBG1, an enzyme that degrades callose at the pores	1421:1521	We found that callose levels are reduced in the qsk1 mutant background with a root phenotype resembling ectopic expression of PdBG1, an enzyme that degrades callose at the pores.
31300470	10	67	theme	development	1858:1868	arg1	regulation					1829:1838	the regulation	1825:1838	the regulation of callose and LR development	1825:1868	Together, the data indicate that reorganization of receptor-like-kinases to plasmodesmata is important for the regulation of callose and LR development as part of the plant response to osmotic stress.
31300470	1	68	theme	environmental	252:264	arg1	stresses					266:273	environmental stresses	252:273	environmental stresses	252:273	Plasmodesmata act as key elements in intercellular communication, coordinating processes related to plant growth, development, and responses to environmental stresses.
31300470	3	69	theme	specific	541:548	arg1	receptor-like-kinases					577:597	specific PM-located Leu-rich-repeat receptor-like-kinases	541:597	specific PM-located Leu-rich-repeat receptor-like-kinases	541:597	Here, we show that specific PM-located Leu-rich-repeat receptor-like-kinases, Qiān Shŏu kinase (QSK1) and inflorescence meristem kinase2, which under optimal growth conditions are absent from plasmodesmata, rapidly relocate and cluster to the pores in response to osmotic stress.
31300470	3	69	theme	specific	541:548	arg1	kinase2					651:657	inflorescence meristem kinase2	628:657	inflorescence meristem kinase2	628:657	Here, we show that specific PM-located Leu-rich-repeat receptor-like-kinases, Qiān Shŏu kinase (QSK1) and inflorescence meristem kinase2, which under optimal growth conditions are absent from plasmodesmata, rapidly relocate and cluster to the pores in response to osmotic stress.
31300470	3	69	theme	specific	541:548	arg1	kinase					610:615	Qiān Shŏu kinase	600:615	Qiān Shŏu kinase (QSK1)	600:622	Here, we show that specific PM-located Leu-rich-repeat receptor-like-kinases, Qiān Shŏu kinase (QSK1) and inflorescence meristem kinase2, which under optimal growth conditions are absent from plasmodesmata, rapidly relocate and cluster to the pores in response to osmotic stress.
31300470	9	70	theme	QSK1	1623:1626	arg1	variants					1628:1635	wild-type and phosphomimic QSK1 variants	1596:1635	wild-type and phosphomimic QSK1 variants	1596:1635	Both the LR and callose phenotypes can be complemented by expression of wild-type and phosphomimic QSK1 variants, but not by phosphodead QSK1 mutant, which fails to relocalize at plasmodesmata.
31300470	3	71	theme	Qiān	600:603	arg1	receptor-like-kinases					577:597	specific PM-located Leu-rich-repeat receptor-like-kinases	541:597	specific PM-located Leu-rich-repeat receptor-like-kinases	541:597	Here, we show that specific PM-located Leu-rich-repeat receptor-like-kinases, Qiān Shŏu kinase (QSK1) and inflorescence meristem kinase2, which under optimal growth conditions are absent from plasmodesmata, rapidly relocate and cluster to the pores in response to osmotic stress.
31300470	3	71	theme	Qiān	600:603	arg1	QSK1					618:621	QSK1	618:621	QSK1	618:621	Here, we show that specific PM-located Leu-rich-repeat receptor-like-kinases, Qiān Shŏu kinase (QSK1) and inflorescence meristem kinase2, which under optimal growth conditions are absent from plasmodesmata, rapidly relocate and cluster to the pores in response to osmotic stress.
31300470	3	71	theme	Qiān	600:603	arg1	kinase					610:615	Qiān Shŏu kinase	600:615	Qiān Shŏu kinase (QSK1)	600:622	Here, we show that specific PM-located Leu-rich-repeat receptor-like-kinases, Qiān Shŏu kinase (QSK1) and inflorescence meristem kinase2, which under optimal growth conditions are absent from plasmodesmata, rapidly relocate and cluster to the pores in response to osmotic stress.
31189465	7	0	from	RESULTS	950:956	arg1	colons					970:975	inflamed colons	961:975	inflamed colons	961:975	RESULTS In inflamed colons, TREM-1 loss compromised the activation of ILC3 and their production of IL-22, which is required for intestinal barrier integrity.
31189465	7	1	theme	IL-22	1049:1053	arg1	activation					1006:1015	the activation	1002:1015	the activation of ILC3	1002:1023	RESULTS In inflamed colons, TREM-1 loss compromised the activation of ILC3 and their production of IL-22, which is required for intestinal barrier integrity.
31189465	7	1	theme	IL-22	1049:1053	arg1	production					1035:1044	their production	1029:1044	their production of IL-22	1029:1053	RESULTS In inflamed colons, TREM-1 loss compromised the activation of ILC3 and their production of IL-22, which is required for intestinal barrier integrity.
31189465	3	2	theme	TREM-1-deficient	544:559	arg1	mice					561:564	TREM-1-deficient mice	544:564	TREM-1-deficient mice	544:564	METHODS Acute colitis was induced in C57BL/6 and TREM-1-deficient mice by the administration of dextran sodium sulfate (DSS).
31189465	5	3	theme	IL-22	786:790	arg1	levels					792:797	IL-22 levels	786:797	IL-22 levels	786:797	An innate lymphoid cell (ILC) co-culture experiment with macrophages was used to analyze IL-22 levels.
31189465	4	4	theme	Colonic	621:627	arg1	propria					636:642	Colonic lamina propria	621:642	Colonic lamina propria	621:642	Colonic lamina propria immune cell composition and cytokines were analyzed.
31189465	8	5	theme	TREM-1	1212:1217	arg1	deficiency					1219:1228	TREM-1 deficiency	1212:1228	TREM-1 deficiency	1212:1228	ILC3-mediated IL-22 production depends on IL-1β secreted by M1-polarized macrophages, and we found that TREM-1 deficiency results in a decreased number of IL-1β producing-M1 macrophages in colons exposed to DSS.
31189465	5	6	theme	innate	700:705	arg1	experiment					738:747	An innate lymphoid cell (ILC) co-culture experiment	697:747	An innate lymphoid cell (ILC) co-culture experiment with macrophages	697:764	An innate lymphoid cell (ILC) co-culture experiment with macrophages was used to analyze IL-22 levels.
31189465	1	7	theme	myeloid	203:209	arg1	TREM-1					220:225	TREM-1	220:225	TREM-1	220:225	BACKGROUND Triggering receptor expressed on myeloid cells-1 (TREM-1) is highly expressed on macrophages in inflamed intestines and reportedly promotes inflammatory bowel disease (IBD) by augmenting pro-inflammatory responses.
31189465	1	7	theme	myeloid	203:209	arg1	cells-1					211:217	myeloid cells-1	203:217	myeloid cells-1 (TREM-1)	203:226	BACKGROUND Triggering receptor expressed on myeloid cells-1 (TREM-1) is highly expressed on macrophages in inflamed intestines and reportedly promotes inflammatory bowel disease (IBD) by augmenting pro-inflammatory responses.
31189465	0	8	theme	IL-22-producing	120:134	arg1	cells					152:156	IL-22-producing innate lymphoid cells	120:156	IL-22-producing innate lymphoid cells	120:156	TREM-1-dependent M1 macrophage polarization restores intestinal epithelium damaged by DSS-induced colitis by activating IL-22-producing innate lymphoid cells.
31189465	8	9	from	number	1253:1258	arg1	colons					1297:1302	colons	1297:1302	colons exposed to DSS	1297:1317	ILC3-mediated IL-22 production depends on IL-1β secreted by M1-polarized macrophages, and we found that TREM-1 deficiency results in a decreased number of IL-1β producing-M1 macrophages in colons exposed to DSS.
31189465	5	10	theme	lymphoid	707:714	arg1	experiment					738:747	An innate lymphoid cell (ILC) co-culture experiment	697:747	An innate lymphoid cell (ILC) co-culture experiment with macrophages	697:764	An innate lymphoid cell (ILC) co-culture experiment with macrophages was used to analyze IL-22 levels.
31189465	6	11	theme	TREM-1-deficient	867:882	arg1	mice					884:887	TREM-1-deficient mice	867:887	TREM-1-deficient mice	867:887	Exogenous IL-22 and TREM-1-expressing macrophages were supplied to TREM-1-deficient mice for examining their effects on intestinal barrier integrity.
31189465	3	12	theme	dextran	591:597	arg1	DSS					615:617	DSS	615:617	DSS	615:617	METHODS Acute colitis was induced in C57BL/6 and TREM-1-deficient mice by the administration of dextran sodium sulfate (DSS).
31189465	3	12	theme	dextran	591:597	arg1	sulfate					606:612	dextran sodium sulfate	591:612	dextran sodium sulfate (DSS)	591:618	METHODS Acute colitis was induced in C57BL/6 and TREM-1-deficient mice by the administration of dextran sodium sulfate (DSS).
31189465	10	13	theme	CONCLUSIONS	1458:1468	arg1	TREM-1					1470:1475	CONCLUSIONS TREM-1	1458:1475	CONCLUSIONS TREM-1	1458:1475	CONCLUSIONS TREM-1 plays a crucial role in regulating IL-22 production by ILC3 through modulating M1-macrophage polarization during DSS-induced acute colitis.
31189465	7	14	theme	inflamed	961:968	arg1	colons					970:975	inflamed colons	961:975	inflamed colons	961:975	RESULTS In inflamed colons, TREM-1 loss compromised the activation of ILC3 and their production of IL-22, which is required for intestinal barrier integrity.
31189465	5	15	used	used	770:773	arg2	experiment					738:747	An innate lymphoid cell (ILC) co-culture experiment	697:747	An innate lymphoid cell (ILC) co-culture experiment with macrophages	697:764	An innate lymphoid cell (ILC) co-culture experiment with macrophages was used to analyze IL-22 levels.
31189465	7	16	theme	ILC3	1020:1023	arg1	activation					1006:1015	the activation	1002:1015	the activation of ILC3	1002:1023	RESULTS In inflamed colons, TREM-1 loss compromised the activation of ILC3 and their production of IL-22, which is required for intestinal barrier integrity.
31189465	7	16	theme	ILC3	1020:1023	arg1	production					1035:1044	their production	1029:1044	their production of IL-22	1029:1053	RESULTS In inflamed colons, TREM-1 loss compromised the activation of ILC3 and their production of IL-22, which is required for intestinal barrier integrity.
31189465	3	17	theme	sodium	599:604	arg1	DSS					615:617	DSS	615:617	DSS	615:617	METHODS Acute colitis was induced in C57BL/6 and TREM-1-deficient mice by the administration of dextran sodium sulfate (DSS).
31189465	3	17	theme	sodium	599:604	arg1	sulfate					606:612	dextran sodium sulfate	591:612	dextran sodium sulfate (DSS)	591:618	METHODS Acute colitis was induced in C57BL/6 and TREM-1-deficient mice by the administration of dextran sodium sulfate (DSS).
31189465	1	18	theme	inflammatory	310:321	arg1	IBD					338:340	IBD	338:340	IBD	338:340	BACKGROUND Triggering receptor expressed on myeloid cells-1 (TREM-1) is highly expressed on macrophages in inflamed intestines and reportedly promotes inflammatory bowel disease (IBD) by augmenting pro-inflammatory responses.
31189465	1	18	theme	inflammatory	310:321	arg1	disease					329:335	inflammatory bowel disease	310:335	inflammatory bowel disease (IBD)	310:341	BACKGROUND Triggering receptor expressed on myeloid cells-1 (TREM-1) is highly expressed on macrophages in inflamed intestines and reportedly promotes inflammatory bowel disease (IBD) by augmenting pro-inflammatory responses.
31189465	0	19	theme	lymphoid	143:150	arg1	cells					152:156	IL-22-producing innate lymphoid cells	120:156	IL-22-producing innate lymphoid cells	120:156	TREM-1-dependent M1 macrophage polarization restores intestinal epithelium damaged by DSS-induced colitis by activating IL-22-producing innate lymphoid cells.
31189465	7	20	theme	intestinal	1078:1087	arg1	integrity					1097:1105	intestinal barrier integrity	1078:1105	intestinal barrier integrity	1078:1105	RESULTS In inflamed colons, TREM-1 loss compromised the activation of ILC3 and their production of IL-22, which is required for intestinal barrier integrity.
31189465	4	21	theme	lamina	629:634	arg1	propria					636:642	Colonic lamina propria	621:642	Colonic lamina propria	621:642	Colonic lamina propria immune cell composition and cytokines were analyzed.
31189465	10	22	theme	M1-macrophage	1556:1568	arg1	polarization					1570:1581	M1-macrophage polarization	1556:1581	M1-macrophage polarization	1556:1581	CONCLUSIONS TREM-1 plays a crucial role in regulating IL-22 production by ILC3 through modulating M1-macrophage polarization during DSS-induced acute colitis.
31189465	8	23	theme	IL-1β	1263:1267	arg1	macrophages					1282:1292	IL-1β producing-M1 macrophages	1263:1292	IL-1β producing-M1 macrophages in colons exposed to DSS	1263:1317	ILC3-mediated IL-22 production depends on IL-1β secreted by M1-polarized macrophages, and we found that TREM-1 deficiency results in a decreased number of IL-1β producing-M1 macrophages in colons exposed to DSS.
31189465	0	24	theme	innate	136:141	arg1	cells					152:156	IL-22-producing innate lymphoid cells	120:156	IL-22-producing innate lymphoid cells	120:156	TREM-1-dependent M1 macrophage polarization restores intestinal epithelium damaged by DSS-induced colitis by activating IL-22-producing innate lymphoid cells.
31189465	9	25	theme	TREM-1-expressing	1402:1418	arg1	macrophages					1420:1430	TREM-1-expressing macrophages	1402:1430	TREM-1-expressing macrophages	1402:1430	Accordingly, DSS-mediated damage was ameliorated by supplying exogenous IL-22 and TREM-1-expressing macrophages to TREM-1-deficient mice.
31189465	8	26	theme	IL-22	1122:1126	arg1	production					1128:1137	ILC3-mediated IL-22 production	1108:1137	ILC3-mediated IL-22 production	1108:1137	ILC3-mediated IL-22 production depends on IL-1β secreted by M1-polarized macrophages, and we found that TREM-1 deficiency results in a decreased number of IL-1β producing-M1 macrophages in colons exposed to DSS.
31189465	6	27	theme	Exogenous	800:808	arg1	IL-22					810:814	Exogenous IL-22	800:814	Exogenous IL-22	800:814	Exogenous IL-22 and TREM-1-expressing macrophages were supplied to TREM-1-deficient mice for examining their effects on intestinal barrier integrity.
31189465	8	28	theme	decreased	1243:1251	arg1	number					1253:1258	a decreased number	1241:1258	a decreased number of IL-1β producing-M1 macrophages in colons exposed to DSS	1241:1317	ILC3-mediated IL-22 production depends on IL-1β secreted by M1-polarized macrophages, and we found that TREM-1 deficiency results in a decreased number of IL-1β producing-M1 macrophages in colons exposed to DSS.
31189465	0	29	theme	M1	17:18	arg1	polarization					31:42	TREM-1-dependent M1 macrophage polarization	0:42	TREM-1-dependent M1 macrophage polarization	0:42	TREM-1-dependent M1 macrophage polarization restores intestinal epithelium damaged by DSS-induced colitis by activating IL-22-producing innate lymphoid cells.
31189465	8	30	from	macrophages	1282:1292	arg1	colons					1297:1302	colons	1297:1302	colons exposed to DSS	1297:1317	ILC3-mediated IL-22 production depends on IL-1β secreted by M1-polarized macrophages, and we found that TREM-1 deficiency results in a decreased number of IL-1β producing-M1 macrophages in colons exposed to DSS.
31189465	10	31	theme	DSS-induced	1590:1600	arg1	colitis					1608:1614	DSS-induced acute colitis	1590:1614	DSS-induced acute colitis	1590:1614	CONCLUSIONS TREM-1 plays a crucial role in regulating IL-22 production by ILC3 through modulating M1-macrophage polarization during DSS-induced acute colitis.
31189465	8	32	from	colons	1297:1302	arg1	number					1253:1258	a decreased number	1241:1258	a decreased number of IL-1β producing-M1 macrophages in colons exposed to DSS	1241:1317	ILC3-mediated IL-22 production depends on IL-1β secreted by M1-polarized macrophages, and we found that TREM-1 deficiency results in a decreased number of IL-1β producing-M1 macrophages in colons exposed to DSS.
31189465	0	33	theme	TREM-1-dependent	0:15	arg1	polarization					31:42	TREM-1-dependent M1 macrophage polarization	0:42	TREM-1-dependent M1 macrophage polarization	0:42	TREM-1-dependent M1 macrophage polarization restores intestinal epithelium damaged by DSS-induced colitis by activating IL-22-producing innate lymphoid cells.
31189465	1	34	theme	bowel	323:327	arg1	IBD					338:340	IBD	338:340	IBD	338:340	BACKGROUND Triggering receptor expressed on myeloid cells-1 (TREM-1) is highly expressed on macrophages in inflamed intestines and reportedly promotes inflammatory bowel disease (IBD) by augmenting pro-inflammatory responses.
31189465	1	34	theme	bowel	323:327	arg1	disease					329:335	inflammatory bowel disease	310:335	inflammatory bowel disease (IBD)	310:341	BACKGROUND Triggering receptor expressed on myeloid cells-1 (TREM-1) is highly expressed on macrophages in inflamed intestines and reportedly promotes inflammatory bowel disease (IBD) by augmenting pro-inflammatory responses.
31189465	6	35	theme	TREM-1-expressing	820:836	arg1	macrophages					838:848	TREM-1-expressing macrophages	820:848	TREM-1-expressing macrophages	820:848	Exogenous IL-22 and TREM-1-expressing macrophages were supplied to TREM-1-deficient mice for examining their effects on intestinal barrier integrity.
31189465	5	36	theme	cell	716:719	arg1	experiment					738:747	An innate lymphoid cell (ILC) co-culture experiment	697:747	An innate lymphoid cell (ILC) co-culture experiment with macrophages	697:764	An innate lymphoid cell (ILC) co-culture experiment with macrophages was used to analyze IL-22 levels.
31189465	2	37	theme	deficient	478:486	arg1	mouse					488:492	an independent TREM-1 deficient mouse	456:492	an independent TREM-1 deficient mouse	456:492	To study the mechanism mediated by TREM-1 on macrophages, we generated an independent TREM-1 deficient mouse.
31189465	9	38	theme	TREM-1-deficient	1435:1450	arg1	mice					1452:1455	TREM-1-deficient mice	1435:1455	TREM-1-deficient mice	1435:1455	Accordingly, DSS-mediated damage was ameliorated by supplying exogenous IL-22 and TREM-1-expressing macrophages to TREM-1-deficient mice.
31189465	10	39	theme	IL-22	1512:1516	arg1	production					1518:1527	IL-22 production	1512:1527	IL-22 production by ILC3	1512:1535	CONCLUSIONS TREM-1 plays a crucial role in regulating IL-22 production by ILC3 through modulating M1-macrophage polarization during DSS-induced acute colitis.
31189465	3	40	theme	sulfate	606:612	arg1	administration					573:586	the administration	569:586	the administration of dextran sodium sulfate (DSS)	569:618	METHODS Acute colitis was induced in C57BL/6 and TREM-1-deficient mice by the administration of dextran sodium sulfate (DSS).
31189465	2	41	theme	independent	459:469	arg1	mouse					488:492	an independent TREM-1 deficient mouse	456:492	an independent TREM-1 deficient mouse	456:492	To study the mechanism mediated by TREM-1 on macrophages, we generated an independent TREM-1 deficient mouse.
31189465	2	42	theme	TREM-1	471:476	arg1	mouse					488:492	an independent TREM-1 deficient mouse	456:492	an independent TREM-1 deficient mouse	456:492	To study the mechanism mediated by TREM-1 on macrophages, we generated an independent TREM-1 deficient mouse.
31189465	4	43	dep	propria	636:642	arg1	composition					656:666	immune cell composition	644:666	immune cell composition	644:666	Colonic lamina propria immune cell composition and cytokines were analyzed.
31189465	10	44	theme	crucial	1485:1491	arg1	role					1493:1496	a crucial role	1483:1496	a crucial role	1483:1496	CONCLUSIONS TREM-1 plays a crucial role in regulating IL-22 production by ILC3 through modulating M1-macrophage polarization during DSS-induced acute colitis.
31189465	0	45	theme	intestinal	53:62	arg1	epithelium					64:73	intestinal epithelium	53:73	intestinal epithelium damaged by DSS-induced colitis by activating IL-22-producing innate lymphoid cells	53:156	TREM-1-dependent M1 macrophage polarization restores intestinal epithelium damaged by DSS-induced colitis by activating IL-22-producing innate lymphoid cells.
31189465	8	46	theme	M1-polarized	1168:1179	arg1	macrophages					1181:1191	M1-polarized macrophages	1168:1191	M1-polarized macrophages	1168:1191	ILC3-mediated IL-22 production depends on IL-1β secreted by M1-polarized macrophages, and we found that TREM-1 deficiency results in a decreased number of IL-1β producing-M1 macrophages in colons exposed to DSS.
31189465	6	47	theme	barrier	931:937	arg1	integrity					939:947	intestinal barrier integrity	920:947	intestinal barrier integrity	920:947	Exogenous IL-22 and TREM-1-expressing macrophages were supplied to TREM-1-deficient mice for examining their effects on intestinal barrier integrity.
31189465	5	48	with	experiment	738:747	arg1	macrophages					754:764	macrophages	754:764	macrophages	754:764	An innate lymphoid cell (ILC) co-culture experiment with macrophages was used to analyze IL-22 levels.
31189465	8	49	theme	macrophages	1282:1292	arg1	number					1253:1258	a decreased number	1241:1258	a decreased number of IL-1β producing-M1 macrophages in colons exposed to DSS	1241:1317	ILC3-mediated IL-22 production depends on IL-1β secreted by M1-polarized macrophages, and we found that TREM-1 deficiency results in a decreased number of IL-1β producing-M1 macrophages in colons exposed to DSS.
31189465	6	50	theme	intestinal	920:929	arg1	integrity					939:947	intestinal barrier integrity	920:947	intestinal barrier integrity	920:947	Exogenous IL-22 and TREM-1-expressing macrophages were supplied to TREM-1-deficient mice for examining their effects on intestinal barrier integrity.
31189465	8	51	theme	producing-M1	1269:1280	arg1	macrophages					1282:1292	IL-1β producing-M1 macrophages	1263:1292	IL-1β producing-M1 macrophages in colons exposed to DSS	1263:1317	ILC3-mediated IL-22 production depends on IL-1β secreted by M1-polarized macrophages, and we found that TREM-1 deficiency results in a decreased number of IL-1β producing-M1 macrophages in colons exposed to DSS.
31189465	3	52	theme	Acute	503:507	arg1	colitis					509:515	Acute colitis	503:515	Acute colitis	503:515	METHODS Acute colitis was induced in C57BL/6 and TREM-1-deficient mice by the administration of dextran sodium sulfate (DSS).
31189465	6	53	from	effects	909:915	arg1	integrity					939:947	intestinal barrier integrity	920:947	intestinal barrier integrity	920:947	Exogenous IL-22 and TREM-1-expressing macrophages were supplied to TREM-1-deficient mice for examining their effects on intestinal barrier integrity.
31189465	2	54	from	TREM-1	420:425	arg1	macrophages					430:440	macrophages	430:440	macrophages	430:440	To study the mechanism mediated by TREM-1 on macrophages, we generated an independent TREM-1 deficient mouse.
31189465	9	55	theme	exogenous	1382:1390	arg1	IL-22					1392:1396	exogenous IL-22	1382:1396	exogenous IL-22	1382:1396	Accordingly, DSS-mediated damage was ameliorated by supplying exogenous IL-22 and TREM-1-expressing macrophages to TREM-1-deficient mice.
31189465	5	56	theme	ILC	722:724	arg1	experiment					738:747	An innate lymphoid cell (ILC) co-culture experiment	697:747	An innate lymphoid cell (ILC) co-culture experiment with macrophages	697:764	An innate lymphoid cell (ILC) co-culture experiment with macrophages was used to analyze IL-22 levels.
31189465	10	57	theme	acute	1602:1606	arg1	colitis					1608:1614	DSS-induced acute colitis	1590:1614	DSS-induced acute colitis	1590:1614	CONCLUSIONS TREM-1 plays a crucial role in regulating IL-22 production by ILC3 through modulating M1-macrophage polarization during DSS-induced acute colitis.
31189465	8	58	theme	ILC3-mediated	1108:1120	arg1	production					1128:1137	ILC3-mediated IL-22 production	1108:1137	ILC3-mediated IL-22 production	1108:1137	ILC3-mediated IL-22 production depends on IL-1β secreted by M1-polarized macrophages, and we found that TREM-1 deficiency results in a decreased number of IL-1β producing-M1 macrophages in colons exposed to DSS.
31189465	7	59	theme	TREM-1	978:983	arg1	loss					985:988	TREM-1 loss	978:988	TREM-1 loss	978:988	RESULTS In inflamed colons, TREM-1 loss compromised the activation of ILC3 and their production of IL-22, which is required for intestinal barrier integrity.
31189465	1	60	theme	pro-inflammatory	357:372	arg1	responses					374:382	pro-inflammatory responses	357:382	pro-inflammatory responses	357:382	BACKGROUND Triggering receptor expressed on myeloid cells-1 (TREM-1) is highly expressed on macrophages in inflamed intestines and reportedly promotes inflammatory bowel disease (IBD) by augmenting pro-inflammatory responses.
31189465	7	61	theme	barrier	1089:1095	arg1	integrity					1097:1105	intestinal barrier integrity	1078:1105	intestinal barrier integrity	1078:1105	RESULTS In inflamed colons, TREM-1 loss compromised the activation of ILC3 and their production of IL-22, which is required for intestinal barrier integrity.
31189465	9	62	theme	DSS-mediated	1333:1344	arg1	damage					1346:1351	DSS-mediated damage	1333:1351	DSS-mediated damage	1333:1351	Accordingly, DSS-mediated damage was ameliorated by supplying exogenous IL-22 and TREM-1-expressing macrophages to TREM-1-deficient mice.
31189465	3	63	dep	METHODS	495:501	arg1	induced					521:527	induced	521:527	was induced in C57BL/6 and TREM-1-deficient mice by the administration of dextran sodium sulfate (DSS)	517:618	METHODS Acute colitis was induced in C57BL/6 and TREM-1-deficient mice by the administration of dextran sodium sulfate (DSS).
31189465	1	64	theme	BACKGROUND	159:168	arg1	receptor					181:188	BACKGROUND Triggering receptor	159:188	BACKGROUND Triggering receptor expressed on myeloid cells-1 (TREM-1)	159:226	BACKGROUND Triggering receptor expressed on myeloid cells-1 (TREM-1) is highly expressed on macrophages in inflamed intestines and reportedly promotes inflammatory bowel disease (IBD) by augmenting pro-inflammatory responses.
31189465	4	65	theme	cell	651:654	arg1	composition					656:666	immune cell composition	644:666	immune cell composition	644:666	Colonic lamina propria immune cell composition and cytokines were analyzed.
31189465	5	66	theme	co-culture	727:736	arg1	experiment					738:747	An innate lymphoid cell (ILC) co-culture experiment	697:747	An innate lymphoid cell (ILC) co-culture experiment with macrophages	697:764	An innate lymphoid cell (ILC) co-culture experiment with macrophages was used to analyze IL-22 levels.
31189465	1	67	theme	Triggering	170:179	arg1	receptor					181:188	BACKGROUND Triggering receptor	159:188	BACKGROUND Triggering receptor expressed on myeloid cells-1 (TREM-1)	159:226	BACKGROUND Triggering receptor expressed on myeloid cells-1 (TREM-1) is highly expressed on macrophages in inflamed intestines and reportedly promotes inflammatory bowel disease (IBD) by augmenting pro-inflammatory responses.
31189465	4	68	theme	immune	644:649	arg1	composition					656:666	immune cell composition	644:666	immune cell composition	644:666	Colonic lamina propria immune cell composition and cytokines were analyzed.
31189465	0	69	theme	DSS-induced	86:96	arg1	colitis					98:104	DSS-induced colitis	86:104	DSS-induced colitis	86:104	TREM-1-dependent M1 macrophage polarization restores intestinal epithelium damaged by DSS-induced colitis by activating IL-22-producing innate lymphoid cells.
31189465	1	70	theme	inflamed	266:273	arg1	intestines					275:284	inflamed intestines	266:284	inflamed intestines	266:284	BACKGROUND Triggering receptor expressed on myeloid cells-1 (TREM-1) is highly expressed on macrophages in inflamed intestines and reportedly promotes inflammatory bowel disease (IBD) by augmenting pro-inflammatory responses.
31189465	0	71	theme	macrophage	20:29	arg1	polarization					31:42	TREM-1-dependent M1 macrophage polarization	0:42	TREM-1-dependent M1 macrophage polarization	0:42	TREM-1-dependent M1 macrophage polarization restores intestinal epithelium damaged by DSS-induced colitis by activating IL-22-producing innate lymphoid cells.
31189465	7	72	dep	compromised	990:1000	arg1	required					1065:1072	required	1065:1072	is required for intestinal barrier integrity	1062:1105	RESULTS In inflamed colons, TREM-1 loss compromised the activation of ILC3 and their production of IL-22, which is required for intestinal barrier integrity.
31050624	9	0	theme	unidentified	1194:1205	arg1	phospholipids					1207:1219	two unidentified phospholipids	1190:1219	two unidentified phospholipids	1190:1219	The polar lipid profile consisted of diphosphadidylglycerol, phosphadidylglycerol, phosphatidylcholine, phosphatidylinositol and two unidentified phospholipids.
31050624	7	1	theme	diagnostic	833:842	arg1	acid					912:915	meso-diaminopimelic acid	892:915	meso-diaminopimelic acid	892:915	The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid.
31050624	7	1	theme	diagnostic	833:842	arg1	acid					852:855	The diagnostic diamino acid	829:855	The diagnostic diamino acid in the cell-wall peptidoglycan	829:886	The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid.
31050624	14	2	theme	type	1583:1586	arg1	GP-T3-3T					1598:1605	GP-T3-3T	1598:1605	GP-T3-3T (=KCTC 49173T=JCM 32825T=KCCM 43309T=KACC 19850T)	1598:1655	The type strain is GP-T3-3T (=KCTC 49173T=JCM 32825T=KCCM 43309T=KACC 19850T).
31050624	14	2	theme	type	1583:1586	arg1	strain					1588:1593	The type strain	1579:1593	The type strain	1579:1593	The type strain is GP-T3-3T (=KCTC 49173T=JCM 32825T=KCCM 43309T=KACC 19850T).
31050624	2	3	theme	bacterial	116:124	arg1	strain					126:131	A novel Gram-strain-positive, non-spore-forming bacterial strain	68:131	A novel Gram-strain-positive, non-spore-forming bacterial strain	68:131	A novel Gram-strain-positive, non-spore-forming bacterial strain, designated GP-T3-3T, was isolated from sediment sampled at a tidal flat in Gopado, Republic of Korea.
31050624	6	4	theme	profundi	707:714	arg1	1A05965T					721:728	Serinicoccus profundi MCCC 1A05965T	694:728	Serinicoccus profundi MCCC 1A05965T (99.1 %)	694:737	The results of the phylogenetic analyses based on 16S rRNA gene sequences indicated that strain GP-T3-3T was closely related to Serinicoccus profundi MCCC 1A05965T (99.1 %), Serinicoccus chungangensis CAU 9536T (99.0 %) and Serinicoccus marinus JC1078T (98.0 %).
31050624	6	4	theme	profundi	707:714	arg1	%					736:736	99.1 %	731:736	99.1 %	731:736	The results of the phylogenetic analyses based on 16S rRNA gene sequences indicated that strain GP-T3-3T was closely related to Serinicoccus profundi MCCC 1A05965T (99.1 %), Serinicoccus chungangensis CAU 9536T (99.0 %) and Serinicoccus marinus JC1078T (98.0 %).
31050624	12	5	theme	Serinicoccussediminis	1535:1555	arg1	sp					1557:1558	the name Serinicoccussediminis sp	1526:1558	the name Serinicoccussediminis sp	1526:1558	On the basis of the phenotypic differences and DNA-DNA relatedness data, the isolate represents a new species of the genus Serinicoccus, for which the name Serinicoccussediminis sp.
31050624	2	6	dep	Gram-strain-positive	76:95	arg1	non-spore-forming					98:114	non-spore-forming	98:114	non-spore-forming	98:114	A novel Gram-strain-positive, non-spore-forming bacterial strain, designated GP-T3-3T, was isolated from sediment sampled at a tidal flat in Gopado, Republic of Korea.
31050624	6	7	theme	Serinicoccus	694:705	arg1	1A05965T					721:728	Serinicoccus profundi MCCC 1A05965T	694:728	Serinicoccus profundi MCCC 1A05965T (99.1 %)	694:737	The results of the phylogenetic analyses based on 16S rRNA gene sequences indicated that strain GP-T3-3T was closely related to Serinicoccus profundi MCCC 1A05965T (99.1 %), Serinicoccus chungangensis CAU 9536T (99.0 %) and Serinicoccus marinus JC1078T (98.0 %).
31050624	6	7	theme	Serinicoccus	694:705	arg1	%					736:736	99.1 %	731:736	99.1 %	731:736	The results of the phylogenetic analyses based on 16S rRNA gene sequences indicated that strain GP-T3-3T was closely related to Serinicoccus profundi MCCC 1A05965T (99.1 %), Serinicoccus chungangensis CAU 9536T (99.0 %) and Serinicoccus marinus JC1078T (98.0 %).
31050624	1	8	theme	tidal	47:51	arg1	sediment					58:65	tidal flat sediment	47:65	tidal flat sediment	47:65	nov., isolated from tidal flat sediment.
31050624	12	9	theme	name	1530:1533	arg1	sp					1557:1558	the name Serinicoccussediminis sp	1526:1558	the name Serinicoccussediminis sp	1526:1558	On the basis of the phenotypic differences and DNA-DNA relatedness data, the isolate represents a new species of the genus Serinicoccus, for which the name Serinicoccussediminis sp.
31050624	7	10	theme	diamino	844:850	arg1	acid					912:915	meso-diaminopimelic acid	892:915	meso-diaminopimelic acid	892:915	The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid.
31050624	7	10	theme	diamino	844:850	arg1	acid					852:855	The diagnostic diamino acid	829:855	The diagnostic diamino acid in the cell-wall peptidoglycan	829:886	The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid.
31050624	10	11	theme	72.9 mol	1246:1253	arg1	%					1254:1254	72.9 mol%	1246:1254	72.9 mol%	1246:1254	The DNA G+C content was 72.9 mol%.
31050624	10	11	theme	72.9 mol	1246:1253	arg1	G+C content					1230:1240	The DNA G+C content	1222:1240	The DNA G+C content	1222:1240	The DNA G+C content was 72.9 mol%.
31050624	1	12	theme	flat	53:56	arg1	sediment					58:65	tidal flat sediment	47:65	tidal flat sediment	47:65	nov., isolated from tidal flat sediment.
31050624	6	13	theme	analyses	598:605	arg1	results					570:576	The results	566:576	The results of the phylogenetic analyses based on 16S rRNA gene sequences	566:638	The results of the phylogenetic analyses based on 16S rRNA gene sequences indicated that strain GP-T3-3T was closely related to Serinicoccus profundi MCCC 1A05965T (99.1 %), Serinicoccus chungangensis CAU 9536T (99.0 %) and Serinicoccus marinus JC1078T (98.0 %).
31050624	2	14	from	flat	201:204	arg1	Republic					217:224	Republic	217:224	Republic	217:224	A novel Gram-strain-positive, non-spore-forming bacterial strain, designated GP-T3-3T, was isolated from sediment sampled at a tidal flat in Gopado, Republic of Korea.
31050624	12	15	theme	Serinicoccus	1502:1513	arg1	species					1481:1487	a new species	1475:1487	a new species	1475:1487	On the basis of the phenotypic differences and DNA-DNA relatedness data, the isolate represents a new species of the genus Serinicoccus, for which the name Serinicoccussediminis sp.
31050624	5	16	theme	GP-T3-3T	507:514	arg1	Colonies					488:495	Colonies	488:495	Colonies of strain GP-T3-3T	488:514	Colonies of strain GP-T3-3T were deep-yellow, circular, smooth and pulvinate.
31050624	5	17	theme	strain	500:505	arg1	GP-T3-3T					507:514	strain GP-T3-3T	500:514	strain GP-T3-3T	500:514	Colonies of strain GP-T3-3T were deep-yellow, circular, smooth and pulvinate.
31050624	6	18	theme	phylogenetic	585:596	arg1	analyses					598:605	the phylogenetic analyses	581:605	the phylogenetic analyses based on 16S rRNA gene sequences	581:638	The results of the phylogenetic analyses based on 16S rRNA gene sequences indicated that strain GP-T3-3T was closely related to Serinicoccus profundi MCCC 1A05965T (99.1 %), Serinicoccus chungangensis CAU 9536T (99.0 %) and Serinicoccus marinus JC1078T (98.0 %).
31050624	11	19	theme	relatedness	1265:1275	arg1	values					1277:1282	DNA-DNA relatedness values	1257:1282	DNA-DNA relatedness values between strain GP-T3-3T and type strains of the genus Serinicoccus	1257:1349	DNA-DNA relatedness values between strain GP-T3-3T and type strains of the genus Serinicoccus ranged from 28.9 to 50.5 %.
31050624	6	20	theme	CAU	767:769	arg1	9536T					771:775	Serinicoccus chungangensis CAU 9536T	740:775	Serinicoccus chungangensis CAU 9536T (99.0 %)	740:784	The results of the phylogenetic analyses based on 16S rRNA gene sequences indicated that strain GP-T3-3T was closely related to Serinicoccus profundi MCCC 1A05965T (99.1 %), Serinicoccus chungangensis CAU 9536T (99.0 %) and Serinicoccus marinus JC1078T (98.0 %).
31050624	6	20	theme	CAU	767:769	arg1	%					783:783	99.0 %	778:783	99.0 %	778:783	The results of the phylogenetic analyses based on 16S rRNA gene sequences indicated that strain GP-T3-3T was closely related to Serinicoccus profundi MCCC 1A05965T (99.1 %), Serinicoccus chungangensis CAU 9536T (99.0 %) and Serinicoccus marinus JC1078T (98.0 %).
31050624	11	21	theme	strain	1292:1297	arg1	GP-T3-3T					1299:1306	strain GP-T3-3T and type strains	1292:1323	GP-T3-3T	1299:1306	DNA-DNA relatedness values between strain GP-T3-3T and type strains of the genus Serinicoccus ranged from 28.9 to 50.5 %.
31050624	2	22	theme	novel	70:74	arg1	strain					126:131	A novel Gram-strain-positive, non-spore-forming bacterial strain	68:131	A novel Gram-strain-positive, non-spore-forming bacterial strain	68:131	A novel Gram-strain-positive, non-spore-forming bacterial strain, designated GP-T3-3T, was isolated from sediment sampled at a tidal flat in Gopado, Republic of Korea.
31050624	0	23	theme	sediminis	13:21	arg1	sp					23:24	Serinicoccus sediminis sp	0:24	Serinicoccus sediminis sp.	0:25	Serinicoccus sediminis sp.
31050624	4	24	theme	pH	426:427	arg1	8.0-9.0					429:435	pH 8.0-9.0	426:435	pH 8.0-9.0	426:435	Strain GP-T3-3T grew at 4-45 °C (optimum, 28-37 °C), at pH 4.0-12.0 (pH 8.0-9.0) and in the presence of 0-15 % (w/v) NaCl (3-5 %).
31050624	4	24	theme	pH	426:427	arg1	pH					413:414	pH 4.0-12.0	413:423	pH 4.0-12.0 (pH 8.0-9.0)	413:436	Strain GP-T3-3T grew at 4-45 °C (optimum, 28-37 °C), at pH 4.0-12.0 (pH 8.0-9.0) and in the presence of 0-15 % (w/v) NaCl (3-5 %).
31050624	11	25	dep	50.5 	1371:1375	arg1	to					1368:1369	to	1368:1369	to	1368:1369	DNA-DNA relatedness values between strain GP-T3-3T and type strains of the genus Serinicoccus ranged from 28.9 to 50.5 %.
31050624	14	26	theme	43309T=KACC	1637:1647	arg1	GP-T3-3T					1598:1605	GP-T3-3T	1598:1605	GP-T3-3T (=KCTC 49173T=JCM 32825T=KCCM 43309T=KACC 19850T)	1598:1655	The type strain is GP-T3-3T (=KCTC 49173T=JCM 32825T=KCCM 43309T=KACC 19850T).
31050624	14	26	theme	43309T=KACC	1637:1647	arg1	19850T					1649:1654	=KCTC 49173T=JCM 32825T=KCCM 43309T=KACC 19850T	1608:1654	=KCTC 49173T=JCM 32825T=KCCM 43309T=KACC 19850T	1608:1654	The type strain is GP-T3-3T (=KCTC 49173T=JCM 32825T=KCCM 43309T=KACC 19850T).
31050624	0	27	theme	Serinicoccus	0:11	arg1	sp					23:24	Serinicoccus sediminis sp	0:24	Serinicoccus sediminis sp.	0:25	Serinicoccus sediminis sp.
31050624	4	28	theme	w/v	469:471	arg1	%					484:484	3-5 %	480:484	3-5 %	480:484	Strain GP-T3-3T grew at 4-45 °C (optimum, 28-37 °C), at pH 4.0-12.0 (pH 8.0-9.0) and in the presence of 0-15 % (w/v) NaCl (3-5 %).
31050624	4	28	theme	w/v	469:471	arg1	NaCl					474:477	0-15 % (w/v) NaCl	461:477	0-15 % (w/v) NaCl (3-5 %)	461:485	Strain GP-T3-3T grew at 4-45 °C (optimum, 28-37 °C), at pH 4.0-12.0 (pH 8.0-9.0) and in the presence of 0-15 % (w/v) NaCl (3-5 %).
31050624	8	29	theme	respiratory	934:944	arg1	MK-8					958:961	MK-8	958:961	MK-8	958:961	The predominant respiratory quinone was MK-8(H4) and the major fatty acids were anteiso-C17 : 0, iso-C16 : 0, iso-C15 : 0 and anteiso-C15 : 0.
31050624	8	29	theme	respiratory	934:944	arg1	quinone					946:952	The predominant respiratory quinone	918:952	The predominant respiratory quinone	918:952	The predominant respiratory quinone was MK-8(H4) and the major fatty acids were anteiso-C17 : 0, iso-C16 : 0, iso-C15 : 0 and anteiso-C15 : 0.
31050624	8	30	theme	predominant	922:932	arg1	MK-8					958:961	MK-8	958:961	MK-8	958:961	The predominant respiratory quinone was MK-8(H4) and the major fatty acids were anteiso-C17 : 0, iso-C16 : 0, iso-C15 : 0 and anteiso-C15 : 0.
31050624	8	30	theme	predominant	922:932	arg1	quinone					946:952	The predominant respiratory quinone	918:952	The predominant respiratory quinone	918:952	The predominant respiratory quinone was MK-8(H4) and the major fatty acids were anteiso-C17 : 0, iso-C16 : 0, iso-C15 : 0 and anteiso-C15 : 0.
31050624	11	31	theme	DNA-DNA	1257:1263	arg1	values					1277:1282	DNA-DNA relatedness values	1257:1282	DNA-DNA relatedness values between strain GP-T3-3T and type strains of the genus Serinicoccus	1257:1349	DNA-DNA relatedness values between strain GP-T3-3T and type strains of the genus Serinicoccus ranged from 28.9 to 50.5 %.
31050624	7	32	from	acid	852:855	arg1	peptidoglycan					874:886	the cell-wall peptidoglycan	860:886	the cell-wall peptidoglycan	860:886	The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid.
31050624	12	33	theme	new	1477:1479	arg1	species					1481:1487	a new species	1475:1487	a new species	1475:1487	On the basis of the phenotypic differences and DNA-DNA relatedness data, the isolate represents a new species of the genus Serinicoccus, for which the name Serinicoccussediminis sp.
31050624	6	34	theme	strain	655:660	arg1	GP-T3-3T					662:669	strain GP-T3-3T	655:669	strain GP-T3-3T	655:669	The results of the phylogenetic analyses based on 16S rRNA gene sequences indicated that strain GP-T3-3T was closely related to Serinicoccus profundi MCCC 1A05965T (99.1 %), Serinicoccus chungangensis CAU 9536T (99.0 %) and Serinicoccus marinus JC1078T (98.0 %).
31050624	11	35	theme	genus	1332:1336	arg1	Serinicoccus					1338:1349	the genus Serinicoccus	1328:1349	the genus Serinicoccus	1328:1349	DNA-DNA relatedness values between strain GP-T3-3T and type strains of the genus Serinicoccus ranged from 28.9 to 50.5 %.
31050624	10	36	theme	DNA	1226:1228	arg1	%					1254:1254	72.9 mol%	1246:1254	72.9 mol%	1246:1254	The DNA G+C content was 72.9 mol%.
31050624	10	36	theme	DNA	1226:1228	arg1	G+C content					1230:1240	The DNA G+C content	1222:1240	The DNA G+C content	1222:1240	The DNA G+C content was 72.9 mol%.
31050624	4	37	theme	NaCl	474:477	arg1	presence					449:456	the presence	445:456	the presence of 0-15 % (w/v) NaCl (3-5 %)	445:485	Strain GP-T3-3T grew at 4-45 °C (optimum, 28-37 °C), at pH 4.0-12.0 (pH 8.0-9.0) and in the presence of 0-15 % (w/v) NaCl (3-5 %).
31050624	6	38	theme	Serinicoccus	740:751	arg1	9536T					771:775	Serinicoccus chungangensis CAU 9536T	740:775	Serinicoccus chungangensis CAU 9536T (99.0 %)	740:784	The results of the phylogenetic analyses based on 16S rRNA gene sequences indicated that strain GP-T3-3T was closely related to Serinicoccus profundi MCCC 1A05965T (99.1 %), Serinicoccus chungangensis CAU 9536T (99.0 %) and Serinicoccus marinus JC1078T (98.0 %).
31050624	6	38	theme	Serinicoccus	740:751	arg1	%					783:783	99.0 %	778:783	99.0 %	778:783	The results of the phylogenetic analyses based on 16S rRNA gene sequences indicated that strain GP-T3-3T was closely related to Serinicoccus profundi MCCC 1A05965T (99.1 %), Serinicoccus chungangensis CAU 9536T (99.0 %) and Serinicoccus marinus JC1078T (98.0 %).
31050624	12	39	dep	differences	1410:1420	arg1	basis					1386:1390	basis	1386:1390	basis	1386:1390	On the basis of the phenotypic differences and DNA-DNA relatedness data, the isolate represents a new species of the genus Serinicoccus, for which the name Serinicoccussediminis sp.
31050624	12	39	dep	differences	1410:1420	arg1	the					1382:1384	the	1382:1384	the	1382:1384	On the basis of the phenotypic differences and DNA-DNA relatedness data, the isolate represents a new species of the genus Serinicoccus, for which the name Serinicoccussediminis sp.
31050624	11	40	theme	type	1312:1315	arg1	strains					1317:1323	strain GP-T3-3T and type strains	1292:1323	strains	1317:1323	DNA-DNA relatedness values between strain GP-T3-3T and type strains of the genus Serinicoccus ranged from 28.9 to 50.5 %.
31050624	6	41	dep	Serinicoccus	790:801	arg1	marinus					803:809	marinus	803:809	marinus	803:809	The results of the phylogenetic analyses based on 16S rRNA gene sequences indicated that strain GP-T3-3T was closely related to Serinicoccus profundi MCCC 1A05965T (99.1 %), Serinicoccus chungangensis CAU 9536T (99.0 %) and Serinicoccus marinus JC1078T (98.0 %).
31050624	14	42	theme	32825T=KCCM	1625:1635	arg1	GP-T3-3T					1598:1605	GP-T3-3T	1598:1605	GP-T3-3T (=KCTC 49173T=JCM 32825T=KCCM 43309T=KACC 19850T)	1598:1655	The type strain is GP-T3-3T (=KCTC 49173T=JCM 32825T=KCCM 43309T=KACC 19850T).
31050624	14	42	theme	32825T=KCCM	1625:1635	arg1	19850T					1649:1654	=KCTC 49173T=JCM 32825T=KCCM 43309T=KACC 19850T	1608:1654	=KCTC 49173T=JCM 32825T=KCCM 43309T=KACC 19850T	1608:1654	The type strain is GP-T3-3T (=KCTC 49173T=JCM 32825T=KCCM 43309T=KACC 19850T).
31050624	2	43	attach	isolated	159:166	arg1	sediment					173:180	sediment	173:180	sediment sampled at a tidal flat in Gopado, Republic of Korea	173:233	A novel Gram-strain-positive, non-spore-forming bacterial strain, designated GP-T3-3T, was isolated from sediment sampled at a tidal flat in Gopado, Republic of Korea.
31050624	2	43	attach	isolated	159:166	arg2	strain					126:131	A novel Gram-strain-positive, non-spore-forming bacterial strain	68:131	A novel Gram-strain-positive, non-spore-forming bacterial strain	68:131	A novel Gram-strain-positive, non-spore-forming bacterial strain, designated GP-T3-3T, was isolated from sediment sampled at a tidal flat in Gopado, Republic of Korea.
31050624	12	44	theme	relatedness	1434:1444	arg1	data					1446:1449	DNA-DNA relatedness data	1426:1449	DNA-DNA relatedness data	1426:1449	On the basis of the phenotypic differences and DNA-DNA relatedness data, the isolate represents a new species of the genus Serinicoccus, for which the name Serinicoccussediminis sp.
31050624	3	45	theme	aerobic	247:253	arg1	cocci					304:308	aerobic, catalase-negative, oxidase-positive, non-motile cocci	247:308	aerobic, catalase-negative, oxidase-positive, non-motile cocci that occurred singly, in pairs or in clusters	247:354	Cells were aerobic, catalase-negative, oxidase-positive, non-motile cocci that occurred singly, in pairs or in clusters.
31050624	12	46	theme	genus	1496:1500	arg1	Serinicoccus					1502:1513	the genus Serinicoccus	1492:1513	the genus Serinicoccus	1492:1513	On the basis of the phenotypic differences and DNA-DNA relatedness data, the isolate represents a new species of the genus Serinicoccus, for which the name Serinicoccussediminis sp.
31050624	7	47	theme	cell-wall	864:872	arg1	peptidoglycan					874:886	the cell-wall peptidoglycan	860:886	the cell-wall peptidoglycan	860:886	The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid.
31050624	14	48	theme	=KCTC	1608:1612	arg1	GP-T3-3T					1598:1605	GP-T3-3T	1598:1605	GP-T3-3T (=KCTC 49173T=JCM 32825T=KCCM 43309T=KACC 19850T)	1598:1655	The type strain is GP-T3-3T (=KCTC 49173T=JCM 32825T=KCCM 43309T=KACC 19850T).
31050624	14	48	theme	=KCTC	1608:1612	arg1	19850T					1649:1654	=KCTC 49173T=JCM 32825T=KCCM 43309T=KACC 19850T	1608:1654	=KCTC 49173T=JCM 32825T=KCCM 43309T=KACC 19850T	1608:1654	The type strain is GP-T3-3T (=KCTC 49173T=JCM 32825T=KCCM 43309T=KACC 19850T).
31050624	2	49	theme	Gram-strain-positive	76:95	arg1	strain					126:131	A novel Gram-strain-positive, non-spore-forming bacterial strain	68:131	A novel Gram-strain-positive, non-spore-forming bacterial strain	68:131	A novel Gram-strain-positive, non-spore-forming bacterial strain, designated GP-T3-3T, was isolated from sediment sampled at a tidal flat in Gopado, Republic of Korea.
31050624	8	50	theme	fatty	981:985	arg1	anteiso-C17 					998:1009	anteiso-C17 	998:1009	anteiso-C17 	998:1009	The predominant respiratory quinone was MK-8(H4) and the major fatty acids were anteiso-C17 : 0, iso-C16 : 0, iso-C15 : 0 and anteiso-C15 : 0.
31050624	8	50	theme	fatty	981:985	arg1	acids					987:991	the major fatty acids	971:991	the major fatty acids	971:991	The predominant respiratory quinone was MK-8(H4) and the major fatty acids were anteiso-C17 : 0, iso-C16 : 0, iso-C15 : 0 and anteiso-C15 : 0.
31050624	4	51	theme	%	466:466	arg1	%					484:484	3-5 %	480:484	3-5 %	480:484	Strain GP-T3-3T grew at 4-45 °C (optimum, 28-37 °C), at pH 4.0-12.0 (pH 8.0-9.0) and in the presence of 0-15 % (w/v) NaCl (3-5 %).
31050624	4	51	theme	%	466:466	arg1	NaCl					474:477	0-15 % (w/v) NaCl	461:477	0-15 % (w/v) NaCl (3-5 %)	461:485	Strain GP-T3-3T grew at 4-45 °C (optimum, 28-37 °C), at pH 4.0-12.0 (pH 8.0-9.0) and in the presence of 0-15 % (w/v) NaCl (3-5 %).
31050624	8	52	dep	anteiso-C17 	998:1009	arg1	 0					1011:1012	 0	1011:1012	 0	1011:1012	The predominant respiratory quinone was MK-8(H4) and the major fatty acids were anteiso-C17 : 0, iso-C16 : 0, iso-C15 : 0 and anteiso-C15 : 0.
31050624	8	52	dep	anteiso-C17 	998:1009	arg1	 0					1057:1058	 0	1057:1058	 0	1057:1058	The predominant respiratory quinone was MK-8(H4) and the major fatty acids were anteiso-C17 : 0, iso-C16 : 0, iso-C15 : 0 and anteiso-C15 : 0.
31050624	8	52	dep	anteiso-C17 	998:1009	arg1	anteiso-C15 					1044:1055	anteiso-C15 	1044:1055	anteiso-C15 	1044:1055	The predominant respiratory quinone was MK-8(H4) and the major fatty acids were anteiso-C17 : 0, iso-C16 : 0, iso-C15 : 0 and anteiso-C15 : 0.
31050624	8	52	dep	anteiso-C17 	998:1009	arg1	 0					1024:1025	 0	1024:1025	 0	1024:1025	The predominant respiratory quinone was MK-8(H4) and the major fatty acids were anteiso-C17 : 0, iso-C16 : 0, iso-C15 : 0 and anteiso-C15 : 0.
31050624	8	52	dep	anteiso-C17 	998:1009	arg1	 0					1037:1038	 0	1037:1038	 0	1037:1038	The predominant respiratory quinone was MK-8(H4) and the major fatty acids were anteiso-C17 : 0, iso-C16 : 0, iso-C15 : 0 and anteiso-C15 : 0.
31050624	9	53	theme	lipid	1071:1075	arg1	profile					1077:1083	The polar lipid profile	1061:1083	The polar lipid profile	1061:1083	The polar lipid profile consisted of diphosphadidylglycerol, phosphadidylglycerol, phosphatidylcholine, phosphatidylinositol and two unidentified phospholipids.
31050624	6	54	theme	Serinicoccus	790:801	arg1	JC1078T					811:817	Serinicoccus marinus JC1078T	790:817	Serinicoccus marinus JC1078T (98.0 %)	790:826	The results of the phylogenetic analyses based on 16S rRNA gene sequences indicated that strain GP-T3-3T was closely related to Serinicoccus profundi MCCC 1A05965T (99.1 %), Serinicoccus chungangensis CAU 9536T (99.0 %) and Serinicoccus marinus JC1078T (98.0 %).
31050624	6	54	theme	Serinicoccus	790:801	arg1	%					825:825	98.0 %	820:825	98.0 %	820:825	The results of the phylogenetic analyses based on 16S rRNA gene sequences indicated that strain GP-T3-3T was closely related to Serinicoccus profundi MCCC 1A05965T (99.1 %), Serinicoccus chungangensis CAU 9536T (99.0 %) and Serinicoccus marinus JC1078T (98.0 %).
31050624	8	55	theme	major	975:979	arg1	anteiso-C17 					998:1009	anteiso-C17 	998:1009	anteiso-C17 	998:1009	The predominant respiratory quinone was MK-8(H4) and the major fatty acids were anteiso-C17 : 0, iso-C16 : 0, iso-C15 : 0 and anteiso-C15 : 0.
31050624	8	55	theme	major	975:979	arg1	acids					987:991	the major fatty acids	971:991	the major fatty acids	971:991	The predominant respiratory quinone was MK-8(H4) and the major fatty acids were anteiso-C17 : 0, iso-C16 : 0, iso-C15 : 0 and anteiso-C15 : 0.
31050624	2	56	theme	tidal	195:199	arg1	flat					201:204	a tidal flat	193:204	a tidal flat in Gopado, Republic of Korea	193:233	A novel Gram-strain-positive, non-spore-forming bacterial strain, designated GP-T3-3T, was isolated from sediment sampled at a tidal flat in Gopado, Republic of Korea.
31050624	6	57	theme	gene	625:628	arg1	sequences					630:638	16S rRNA gene sequences	616:638	16S rRNA gene sequences	616:638	The results of the phylogenetic analyses based on 16S rRNA gene sequences indicated that strain GP-T3-3T was closely related to Serinicoccus profundi MCCC 1A05965T (99.1 %), Serinicoccus chungangensis CAU 9536T (99.0 %) and Serinicoccus marinus JC1078T (98.0 %).
31050624	14	58	theme	49173T=JCM	1614:1623	arg1	GP-T3-3T					1598:1605	GP-T3-3T	1598:1605	GP-T3-3T (=KCTC 49173T=JCM 32825T=KCCM 43309T=KACC 19850T)	1598:1655	The type strain is GP-T3-3T (=KCTC 49173T=JCM 32825T=KCCM 43309T=KACC 19850T).
31050624	14	58	theme	49173T=JCM	1614:1623	arg1	19850T					1649:1654	=KCTC 49173T=JCM 32825T=KCCM 43309T=KACC 19850T	1608:1654	=KCTC 49173T=JCM 32825T=KCCM 43309T=KACC 19850T	1608:1654	The type strain is GP-T3-3T (=KCTC 49173T=JCM 32825T=KCCM 43309T=KACC 19850T).
31050624	12	59	theme	phenotypic	1399:1408	arg1	differences					1410:1420	the phenotypic differences	1395:1420	the phenotypic differences	1395:1420	On the basis of the phenotypic differences and DNA-DNA relatedness data, the isolate represents a new species of the genus Serinicoccus, for which the name Serinicoccussediminis sp.
31050624	7	60	theme	meso-diaminopimelic	892:910	arg1	acid					912:915	meso-diaminopimelic acid	892:915	meso-diaminopimelic acid	892:915	The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid.
31050624	7	60	theme	meso-diaminopimelic	892:910	arg1	acid					852:855	The diagnostic diamino acid	829:855	The diagnostic diamino acid in the cell-wall peptidoglycan	829:886	The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid.
31050624	6	61	theme	rRNA	620:623	arg1	sequences					630:638	16S rRNA gene sequences	616:638	16S rRNA gene sequences	616:638	The results of the phylogenetic analyses based on 16S rRNA gene sequences indicated that strain GP-T3-3T was closely related to Serinicoccus profundi MCCC 1A05965T (99.1 %), Serinicoccus chungangensis CAU 9536T (99.0 %) and Serinicoccus marinus JC1078T (98.0 %).
31050624	9	62	theme	polar	1065:1069	arg1	profile					1077:1083	The polar lipid profile	1061:1083	The polar lipid profile	1061:1083	The polar lipid profile consisted of diphosphadidylglycerol, phosphadidylglycerol, phosphatidylcholine, phosphatidylinositol and two unidentified phospholipids.
31050624	11	63	theme	Serinicoccus	1338:1349	arg1	GP-T3-3T					1299:1306	strain GP-T3-3T and type strains	1292:1323	GP-T3-3T	1299:1306	DNA-DNA relatedness values between strain GP-T3-3T and type strains of the genus Serinicoccus ranged from 28.9 to 50.5 %.
31050624	11	63	theme	Serinicoccus	1338:1349	arg1	strains					1317:1323	strain GP-T3-3T and type strains	1292:1323	strains	1317:1323	DNA-DNA relatedness values between strain GP-T3-3T and type strains of the genus Serinicoccus ranged from 28.9 to 50.5 %.
31050624	6	64	theme	MCCC	716:719	arg1	1A05965T					721:728	Serinicoccus profundi MCCC 1A05965T	694:728	Serinicoccus profundi MCCC 1A05965T (99.1 %)	694:737	The results of the phylogenetic analyses based on 16S rRNA gene sequences indicated that strain GP-T3-3T was closely related to Serinicoccus profundi MCCC 1A05965T (99.1 %), Serinicoccus chungangensis CAU 9536T (99.0 %) and Serinicoccus marinus JC1078T (98.0 %).
31050624	6	64	theme	MCCC	716:719	arg1	%					736:736	99.1 %	731:736	99.1 %	731:736	The results of the phylogenetic analyses based on 16S rRNA gene sequences indicated that strain GP-T3-3T was closely related to Serinicoccus profundi MCCC 1A05965T (99.1 %), Serinicoccus chungangensis CAU 9536T (99.0 %) and Serinicoccus marinus JC1078T (98.0 %).
31050624	6	65	theme	16S	616:618	arg1	sequences					630:638	16S rRNA gene sequences	616:638	16S rRNA gene sequences	616:638	The results of the phylogenetic analyses based on 16S rRNA gene sequences indicated that strain GP-T3-3T was closely related to Serinicoccus profundi MCCC 1A05965T (99.1 %), Serinicoccus chungangensis CAU 9536T (99.0 %) and Serinicoccus marinus JC1078T (98.0 %).
31050624	4	66	dep	optimum	390:396	arg1	28-37 °C					399:406	28-37 °C	399:406	28-37 °C	399:406	Strain GP-T3-3T grew at 4-45 °C (optimum, 28-37 °C), at pH 4.0-12.0 (pH 8.0-9.0) and in the presence of 0-15 % (w/v) NaCl (3-5 %).
31050624	3	67	dep	aerobic	247:253	arg1	oxidase-positive					275:290	oxidase-positive	275:290	oxidase-positive	275:290	Cells were aerobic, catalase-negative, oxidase-positive, non-motile cocci that occurred singly, in pairs or in clusters.
31050624	3	67	dep	aerobic	247:253	arg1	catalase-negative					256:272	catalase-negative	256:272	catalase-negative	256:272	Cells were aerobic, catalase-negative, oxidase-positive, non-motile cocci that occurred singly, in pairs or in clusters.
31050624	3	67	dep	aerobic	247:253	arg1	non-motile					293:302	non-motile	293:302	non-motile	293:302	Cells were aerobic, catalase-negative, oxidase-positive, non-motile cocci that occurred singly, in pairs or in clusters.
31050624	6	68	dep	Serinicoccus	740:751	arg1	chungangensis					753:765	chungangensis	753:765	chungangensis	753:765	The results of the phylogenetic analyses based on 16S rRNA gene sequences indicated that strain GP-T3-3T was closely related to Serinicoccus profundi MCCC 1A05965T (99.1 %), Serinicoccus chungangensis CAU 9536T (99.0 %) and Serinicoccus marinus JC1078T (98.0 %).
31050624	12	69	theme	DNA-DNA	1426:1432	arg1	data					1446:1449	DNA-DNA relatedness data	1426:1449	DNA-DNA relatedness data	1426:1449	On the basis of the phenotypic differences and DNA-DNA relatedness data, the isolate represents a new species of the genus Serinicoccus, for which the name Serinicoccussediminis sp.
31585749	3	0	theme	Rousettus	209:217	arg1	aegyptiacus					219:229	Rousettus aegyptiacus	209:229	Rousettus aegyptiacus	209:229	Fifteen bifidobacterial strains were obtained from faeces of Rousettus aegyptiacus; after grouping them by RAPD PCR only eight were selected and characterized.
31585749	4	1	theme	Bifidobacterium	774:788	arg1	100201T					748:754	Bifidobacterium tissieri DSM 100201T	719:754	Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%)	719:771	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16T), Cluster II (RST 9T and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685T (96.3%), Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%), Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%), Bifidobacterium reuteri DSM 23975 T (98.9%) and Bifidobacterium myosotis DSM 100196T (99.3%), respectively.
31585749	4	1	theme	Bifidobacterium	774:788	arg1	T					808:808	Bifidobacterium reuteri DSM 23975 T	774:808	Bifidobacterium reuteri DSM 23975 T	774:808	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16T), Cluster II (RST 9T and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685T (96.3%), Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%), Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%), Bifidobacterium reuteri DSM 23975 T (98.9%) and Bifidobacterium myosotis DSM 100196T (99.3%), respectively.
31585749	4	2	dep	genes	387:391	arg1	hsp60					355:359	hsp60	355:359	hsp60	355:359	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16T), Cluster II (RST 9T and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685T (96.3%), Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%), Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%), Bifidobacterium reuteri DSM 23975 T (98.9%) and Bifidobacterium myosotis DSM 100196T (99.3%), respectively.
31585749	4	3	theme	DSM	798:800	arg1	100201T					748:754	Bifidobacterium tissieri DSM 100201T	719:754	Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%)	719:771	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16T), Cluster II (RST 9T and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685T (96.3%), Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%), Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%), Bifidobacterium reuteri DSM 23975 T (98.9%) and Bifidobacterium myosotis DSM 100196T (99.3%), respectively.
31585749	4	3	theme	DSM	798:800	arg1	T					808:808	Bifidobacterium reuteri DSM 23975 T	774:808	Bifidobacterium reuteri DSM 23975 T	774:808	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16T), Cluster II (RST 9T and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685T (96.3%), Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%), Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%), Bifidobacterium reuteri DSM 23975 T (98.9%) and Bifidobacterium myosotis DSM 100196T (99.3%), respectively.
31585749	8	4	theme	Cluster	1357:1363	arg1	I					1365:1365	Cluster I	1357:1365	Cluster I	1357:1365	Phenotypic and genotypic tests demonstrated that strains in Cluster I and RST 9T in Cluster II represent two novel species for which the names Bifidobacterium vespertilionis sp.
31585749	4	5	theme	avesanii	630:637	arg1	100685T					643:649	Bifidobacterium avesanii DSM 100685T	614:649	Bifidobacterium avesanii DSM 100685T	614:649	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16T), Cluster II (RST 9T and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685T (96.3%), Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%), Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%), Bifidobacterium reuteri DSM 23975 T (98.9%) and Bifidobacterium myosotis DSM 100196T (99.3%), respectively.
31585749	4	6	theme	DSM	847:849	arg1	100196T					851:857	Bifidobacterium myosotis DSM 100196T	822:857	Bifidobacterium myosotis DSM 100196T (99.3%)	822:865	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16T), Cluster II (RST 9T and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685T (96.3%), Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%), Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%), Bifidobacterium reuteri DSM 23975 T (98.9%) and Bifidobacterium myosotis DSM 100196T (99.3%), respectively.
31585749	4	6	theme	DSM	847:849	arg1	%					864:864	99.3%	860:864	99.3%	860:864	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16T), Cluster II (RST 9T and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685T (96.3%), Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%), Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%), Bifidobacterium reuteri DSM 23975 T (98.9%) and Bifidobacterium myosotis DSM 100196T (99.3%), respectively.
31585749	10	7	theme	RST	1597:1599	arg1	nov					1591:1593	nov	1591:1593	nov	1591:1593	nov. (RST 9T=BCRC 81136T=NBRC 113378T=DSM 106027T) are proposed.
31585749	10	7	theme	RST	1597:1599	arg1	106027T					1633:1639	RST 9T=BCRC 81136T=NBRC 113378T=DSM 106027T	1597:1639	RST 9T=BCRC 81136T=NBRC 113378T=DSM 106027T	1597:1639	nov. (RST 9T=BCRC 81136T=NBRC 113378T=DSM 106027T) are proposed.
31585749	5	8	from	Strains	882:888	arg1	I					901:901	Cluster I	893:901	Cluster I	893:901	Strains in Cluster I and strain RST 9 in Cluster II could not be placed within any recognized species while the other ones were identified as known species.
31585749	5	8	from	Strains	882:888	arg1	RST					914:916	strain RST 9	907:918	strain RST 9	907:918	Strains in Cluster I and strain RST 9 in Cluster II could not be placed within any recognized species while the other ones were identified as known species.
31585749	5	9	from	RST	914:916	arg1	Cluster					923:929	Cluster II	923:932	Cluster II	923:932	Strains in Cluster I and strain RST 9 in Cluster II could not be placed within any recognized species while the other ones were identified as known species.
31585749	4	10	dep	closest	603:609	arg1	100196T					851:857	Bifidobacterium myosotis DSM 100196T	822:857	Bifidobacterium myosotis DSM 100196T (99.3%)	822:865	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16T), Cluster II (RST 9T and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685T (96.3%), Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%), Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%), Bifidobacterium reuteri DSM 23975 T (98.9%) and Bifidobacterium myosotis DSM 100196T (99.3%), respectively.
31585749	4	10	dep	closest	603:609	arg1	%					770:770	99.7 and 99.2%	757:770	99.7 and 99.2%	757:770	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16T), Cluster II (RST 9T and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685T (96.3%), Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%), Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%), Bifidobacterium reuteri DSM 23975 T (98.9%) and Bifidobacterium myosotis DSM 100196T (99.3%), respectively.
31585749	4	10	dep	closest	603:609	arg1	RST					474:476	RST	474:476	RST	474:476	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16T), Cluster II (RST 9T and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685T (96.3%), Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%), Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%), Bifidobacterium reuteri DSM 23975 T (98.9%) and Bifidobacterium myosotis DSM 100196T (99.3%), respectively.
31585749	4	10	dep	closest	603:609	arg1	8					478:478	8	478:478	8	478:478	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16T), Cluster II (RST 9T and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685T (96.3%), Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%), Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%), Bifidobacterium reuteri DSM 23975 T (98.9%) and Bifidobacterium myosotis DSM 100196T (99.3%), respectively.
31585749	4	10	dep	closest	603:609	arg1	Cluster					494:500	Cluster	494:500	Cluster	494:500	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16T), Cluster II (RST 9T and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685T (96.3%), Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%), Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%), Bifidobacterium reuteri DSM 23975 T (98.9%) and Bifidobacterium myosotis DSM 100196T (99.3%), respectively.
31585749	4	10	dep	closest	603:609	arg1	II					502:503	II	502:503	II	502:503	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16T), Cluster II (RST 9T and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685T (96.3%), Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%), Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%), Bifidobacterium reuteri DSM 23975 T (98.9%) and Bifidobacterium myosotis DSM 100196T (99.3%), respectively.
31585749	4	10	dep	closest	603:609	arg1	%					864:864	99.3%	860:864	99.3%	860:864	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16T), Cluster II (RST 9T and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685T (96.3%), Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%), Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%), Bifidobacterium reuteri DSM 23975 T (98.9%) and Bifidobacterium myosotis DSM 100196T (99.3%), respectively.
31585749	4	10	dep	closest	603:609	arg1	T					808:808	Bifidobacterium reuteri DSM 23975 T	774:808	Bifidobacterium reuteri DSM 23975 T	774:808	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16T), Cluster II (RST 9T and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685T (96.3%), Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%), Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%), Bifidobacterium reuteri DSM 23975 T (98.9%) and Bifidobacterium myosotis DSM 100196T (99.3%), respectively.
31585749	4	10	dep	closest	603:609	arg1	100201T					748:754	Bifidobacterium tissieri DSM 100201T	719:754	Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%)	719:771	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16T), Cluster II (RST 9T and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685T (96.3%), Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%), Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%), Bifidobacterium reuteri DSM 23975 T (98.9%) and Bifidobacterium myosotis DSM 100196T (99.3%), respectively.
31585749	4	10	dep	closest	603:609	arg1	%					656:656	96.3%	652:656	96.3%	652:656	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16T), Cluster II (RST 9T and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685T (96.3%), Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%), Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%), Bifidobacterium reuteri DSM 23975 T (98.9%) and Bifidobacterium myosotis DSM 100196T (99.3%), respectively.
31585749	4	10	dep	closest	603:609	arg1	16T					488:490	16T	488:490	16T	488:490	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16T), Cluster II (RST 9T and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685T (96.3%), Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%), Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%), Bifidobacterium reuteri DSM 23975 T (98.9%) and Bifidobacterium myosotis DSM 100196T (99.3%), respectively.
31585749	4	10	dep	closest	603:609	arg1	23973T					693:698	Bifidobacterium callitrichos DSM 23973T	660:698	Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%)	660:716	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16T), Cluster II (RST 9T and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685T (96.3%), Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%), Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%), Bifidobacterium reuteri DSM 23975 T (98.9%) and Bifidobacterium myosotis DSM 100196T (99.3%), respectively.
31585749	4	10	dep	closest	603:609	arg1	%					815:815	98.9%	811:815	98.9%	811:815	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16T), Cluster II (RST 9T and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685T (96.3%), Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%), Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%), Bifidobacterium reuteri DSM 23975 T (98.9%) and Bifidobacterium myosotis DSM 100196T (99.3%), respectively.
31585749	9	11	theme	RST	1528:1530	arg1	113377					1550:1555	RST 8=BCRC 81135=NBRC 113377	1528:1555	RST 16T=BCRC 81138T=NBRC 113380T=DSM 106025T ; RST 8=BCRC 81135=NBRC 113377	1481:1555	nov. (RST 16T=BCRC 81138T=NBRC 113380T=DSM 106025T ; RST 8=BCRC 81135=NBRC 113377) and Bifidobacterium rousetti sp.
31585749	4	12	theme	Bifidobacterium	822:836	arg1	100196T					851:857	Bifidobacterium myosotis DSM 100196T	822:857	Bifidobacterium myosotis DSM 100196T (99.3%)	822:865	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16T), Cluster II (RST 9T and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685T (96.3%), Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%), Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%), Bifidobacterium reuteri DSM 23975 T (98.9%) and Bifidobacterium myosotis DSM 100196T (99.3%), respectively.
31585749	4	12	theme	Bifidobacterium	822:836	arg1	%					864:864	99.3%	860:864	99.3%	860:864	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16T), Cluster II (RST 9T and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685T (96.3%), Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%), Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%), Bifidobacterium reuteri DSM 23975 T (98.9%) and Bifidobacterium myosotis DSM 100196T (99.3%), respectively.
31585749	4	13	theme	Bifidobacterium	660:674	arg1	23973T					693:698	Bifidobacterium callitrichos DSM 23973T	660:698	Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%)	660:716	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16T), Cluster II (RST 9T and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685T (96.3%), Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%), Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%), Bifidobacterium reuteri DSM 23975 T (98.9%) and Bifidobacterium myosotis DSM 100196T (99.3%), respectively.
31585749	4	14	theme	genes	387:391	arg1	Analysis					308:315	Analysis	308:315	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes	308:391	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16T), Cluster II (RST 9T and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685T (96.3%), Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%), Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%), Bifidobacterium reuteri DSM 23975 T (98.9%) and Bifidobacterium myosotis DSM 100196T (99.3%), respectively.
31585749	10	15	theme	81136T=NBRC	1609:1619	arg1	nov					1591:1593	nov	1591:1593	nov	1591:1593	nov. (RST 9T=BCRC 81136T=NBRC 113378T=DSM 106027T) are proposed.
31585749	10	15	theme	81136T=NBRC	1609:1619	arg1	106027T					1633:1639	RST 9T=BCRC 81136T=NBRC 113378T=DSM 106027T	1597:1639	RST 9T=BCRC 81136T=NBRC 113378T=DSM 106027T	1597:1639	nov. (RST 9T=BCRC 81136T=NBRC 113378T=DSM 106027T) are proposed.
31585749	7	16	dep	In	1195:1196	arg1	silico					1198:1203	silico	1198:1203	silico	1198:1203	In silico DNA-DNA hybridization values for those closest relatives were 32.5 and 42.1%, respectively.
31585749	8	17	theme	novel	1406:1410	arg1	species					1412:1418	two novel species	1402:1418	two novel species for which the names Bifidobacterium vespertilionis sp	1402:1472	Phenotypic and genotypic tests demonstrated that strains in Cluster I and RST 9T in Cluster II represent two novel species for which the names Bifidobacterium vespertilionis sp.
31585749	4	18	theme	DSM	689:691	arg1	23973T					693:698	Bifidobacterium callitrichos DSM 23973T	660:698	Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%)	660:716	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16T), Cluster II (RST 9T and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685T (96.3%), Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%), Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%), Bifidobacterium reuteri DSM 23975 T (98.9%) and Bifidobacterium myosotis DSM 100196T (99.3%), respectively.
31585749	9	19	dep	sp	1587:1588	arg1	nov					1475:1477	nov	1475:1477	nov	1475:1477	nov. (RST 16T=BCRC 81138T=NBRC 113380T=DSM 106025T ; RST 8=BCRC 81135=NBRC 113377) and Bifidobacterium rousetti sp.
31585749	9	19	dep	sp	1587:1588	arg1	106025T					1518:1524	RST 16T=BCRC 81138T=NBRC 113380T=DSM 106025T	1481:1524	RST 16T=BCRC 81138T=NBRC 113380T=DSM 106025T ; RST 8=BCRC 81135=NBRC 113377	1481:1555	nov. (RST 16T=BCRC 81138T=NBRC 113380T=DSM 106025T ; RST 8=BCRC 81135=NBRC 113377) and Bifidobacterium rousetti sp.
31585749	9	19	dep	sp	1587:1588	arg1	rousetti					1578:1585	Bifidobacterium rousetti	1562:1585	Bifidobacterium rousetti	1562:1585	nov. (RST 16T=BCRC 81138T=NBRC 113380T=DSM 106025T ; RST 8=BCRC 81135=NBRC 113377) and Bifidobacterium rousetti sp.
31585749	7	20	theme	closest	1244:1250	arg1	relatives					1252:1260	those closest relatives	1238:1260	those closest relatives	1238:1260	In silico DNA-DNA hybridization values for those closest relatives were 32.5 and 42.1%, respectively.
31585749	4	21	theme	Bifidobacterium	719:733	arg1	%					770:770	99.7 and 99.2%	757:770	99.7 and 99.2%	757:770	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16T), Cluster II (RST 9T and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685T (96.3%), Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%), Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%), Bifidobacterium reuteri DSM 23975 T (98.9%) and Bifidobacterium myosotis DSM 100196T (99.3%), respectively.
31585749	4	21	theme	Bifidobacterium	719:733	arg1	100201T					748:754	Bifidobacterium tissieri DSM 100201T	719:754	Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%)	719:771	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16T), Cluster II (RST 9T and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685T (96.3%), Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%), Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%), Bifidobacterium reuteri DSM 23975 T (98.9%) and Bifidobacterium myosotis DSM 100196T (99.3%), respectively.
31585749	4	21	theme	Bifidobacterium	719:733	arg1	T					808:808	Bifidobacterium reuteri DSM 23975 T	774:808	Bifidobacterium reuteri DSM 23975 T	774:808	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16T), Cluster II (RST 9T and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685T (96.3%), Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%), Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%), Bifidobacterium reuteri DSM 23975 T (98.9%) and Bifidobacterium myosotis DSM 100196T (99.3%), respectively.
31585749	4	21	theme	Bifidobacterium	719:733	arg1	%					815:815	98.9%	811:815	98.9%	811:815	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16T), Cluster II (RST 9T and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685T (96.3%), Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%), Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%), Bifidobacterium reuteri DSM 23975 T (98.9%) and Bifidobacterium myosotis DSM 100196T (99.3%), respectively.
31585749	9	22	theme	8=BCRC	1532:1537	arg1	113377					1550:1555	RST 8=BCRC 81135=NBRC 113377	1528:1555	RST 16T=BCRC 81138T=NBRC 113380T=DSM 106025T ; RST 8=BCRC 81135=NBRC 113377	1481:1555	nov. (RST 16T=BCRC 81138T=NBRC 113380T=DSM 106025T ; RST 8=BCRC 81135=NBRC 113377) and Bifidobacterium rousetti sp.
31585749	0	23	theme	Egyptian	61:68	arg1	bat					76:78	the Egyptian fruit bat	57:78	the Egyptian fruit bat	57:78	Characterization of Bifidobacterium species in feaces of the Egyptian fruit bat: Description of B. vespertilionis sp.
31585749	6	24	theme	RST	1105:1107	arg1	strains					1096:1102	two novel strains	1086:1102	two novel strains	1086:1102	The average nucleotide identity values between two novel strains, RST 16T and RST 9T and their closest relatives were lower than 79% and 89%, respectively.
31585749	6	24	theme	RST	1105:1107	arg1	16T					1109:1111	RST 16T	1105:1111	RST 16T	1105:1111	The average nucleotide identity values between two novel strains, RST 16T and RST 9T and their closest relatives were lower than 79% and 89%, respectively.
31585749	1	25	theme	rousetti	130:137	arg1	sp					139:140	B. rousetti sp	127:140	B. rousetti sp	127:140	nov. and B. rousetti sp.
31585749	4	26	theme	DSM	744:746	arg1	%					770:770	99.7 and 99.2%	757:770	99.7 and 99.2%	757:770	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16T), Cluster II (RST 9T and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685T (96.3%), Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%), Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%), Bifidobacterium reuteri DSM 23975 T (98.9%) and Bifidobacterium myosotis DSM 100196T (99.3%), respectively.
31585749	4	26	theme	DSM	744:746	arg1	100201T					748:754	Bifidobacterium tissieri DSM 100201T	719:754	Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%)	719:771	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16T), Cluster II (RST 9T and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685T (96.3%), Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%), Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%), Bifidobacterium reuteri DSM 23975 T (98.9%) and Bifidobacterium myosotis DSM 100196T (99.3%), respectively.
31585749	4	26	theme	DSM	744:746	arg1	T					808:808	Bifidobacterium reuteri DSM 23975 T	774:808	Bifidobacterium reuteri DSM 23975 T	774:808	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16T), Cluster II (RST 9T and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685T (96.3%), Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%), Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%), Bifidobacterium reuteri DSM 23975 T (98.9%) and Bifidobacterium myosotis DSM 100196T (99.3%), respectively.
31585749	4	26	theme	DSM	744:746	arg1	%					815:815	98.9%	811:815	98.9%	811:815	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16T), Cluster II (RST 9T and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685T (96.3%), Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%), Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%), Bifidobacterium reuteri DSM 23975 T (98.9%) and Bifidobacterium myosotis DSM 100196T (99.3%), respectively.
31585749	0	27	theme	bat	76:78	arg1	feaces					47:52	feaces	47:52	feaces of the Egyptian fruit bat	47:78	Characterization of Bifidobacterium species in feaces of the Egyptian fruit bat: Description of B. vespertilionis sp.
31585749	8	28	theme	Bifidobacterium	1440:1454	arg1	sp					1471:1472	Bifidobacterium vespertilionis sp	1440:1472	Bifidobacterium vespertilionis sp	1440:1472	Phenotypic and genotypic tests demonstrated that strains in Cluster I and RST 9T in Cluster II represent two novel species for which the names Bifidobacterium vespertilionis sp.
31585749	7	29	theme	hybridization	1213:1225	arg1	values					1227:1232	In silico DNA-DNA hybridization values	1195:1232	In silico DNA-DNA hybridization values for those closest relatives	1195:1260	In silico DNA-DNA hybridization values for those closest relatives were 32.5 and 42.1%, respectively.
31585749	0	30	from	Characterization	0:15	arg1	feaces					47:52	feaces	47:52	feaces of the Egyptian fruit bat	47:78	Characterization of Bifidobacterium species in feaces of the Egyptian fruit bat: Description of B. vespertilionis sp.
31585749	3	31	theme	bifidobacterial	156:170	arg1	strains					172:178	Fifteen bifidobacterial strains	148:178	Fifteen bifidobacterial strains	148:178	Fifteen bifidobacterial strains were obtained from faeces of Rousettus aegyptiacus; after grouping them by RAPD PCR only eight were selected and characterized.
31585749	4	32	theme	Cluster	579:585	arg1	RST					590:592	RST 17	590:595	RST 17	590:595	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16T), Cluster II (RST 9T and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685T (96.3%), Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%), Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%), Bifidobacterium reuteri DSM 23975 T (98.9%) and Bifidobacterium myosotis DSM 100196T (99.3%), respectively.
31585749	4	32	theme	Cluster	579:585	arg1	V					587:587	Cluster V	579:587	Cluster V (RST 17)	579:596	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16T), Cluster II (RST 9T and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685T (96.3%), Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%), Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%), Bifidobacterium reuteri DSM 23975 T (98.9%) and Bifidobacterium myosotis DSM 100196T (99.3%), respectively.
31585749	4	32	theme	Cluster	579:585	arg1	Cluster					526:532	Cluster III	526:536	Cluster III (RST 7 and RST 11)	526:555	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16T), Cluster II (RST 9T and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685T (96.3%), Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%), Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%), Bifidobacterium reuteri DSM 23975 T (98.9%) and Bifidobacterium myosotis DSM 100196T (99.3%), respectively.
31585749	0	33	theme	B.	96:97	arg1	sp					114:115	B. vespertilionis sp	96:115	B. vespertilionis sp	96:115	Characterization of Bifidobacterium species in feaces of the Egyptian fruit bat: Description of B. vespertilionis sp.
31585749	8	34	from	strains	1346:1352	arg1	I					1365:1365	Cluster I	1357:1365	Cluster I	1357:1365	Phenotypic and genotypic tests demonstrated that strains in Cluster I and RST 9T in Cluster II represent two novel species for which the names Bifidobacterium vespertilionis sp.
31585749	8	34	from	strains	1346:1352	arg1	9T					1375:1376	RST 9T	1371:1376	RST 9T in Cluster II	1371:1390	Phenotypic and genotypic tests demonstrated that strains in Cluster I and RST 9T in Cluster II represent two novel species for which the names Bifidobacterium vespertilionis sp.
31585749	5	35	theme	known	1024:1028	arg1	species					1030:1036	known species	1024:1036	known species	1024:1036	Strains in Cluster I and strain RST 9 in Cluster II could not be placed within any recognized species while the other ones were identified as known species.
31585749	5	35	theme	known	1024:1028	arg1	ones					1000:1003	the other ones	990:1003	the other ones	990:1003	Strains in Cluster I and strain RST 9 in Cluster II could not be placed within any recognized species while the other ones were identified as known species.
31585749	9	36	dep	106025T	1518:1524	arg1	113377					1550:1555	RST 8=BCRC 81135=NBRC 113377	1528:1555	RST 16T=BCRC 81138T=NBRC 113380T=DSM 106025T ; RST 8=BCRC 81135=NBRC 113377	1481:1555	nov. (RST 16T=BCRC 81138T=NBRC 113380T=DSM 106025T ; RST 8=BCRC 81135=NBRC 113377) and Bifidobacterium rousetti sp.
31585749	0	37	theme	Bifidobacterium	20:34	arg1	species					36:42	Bifidobacterium species	20:42	Bifidobacterium species	20:42	Characterization of Bifidobacterium species in feaces of the Egyptian fruit bat: Description of B. vespertilionis sp.
31585749	4	38	dep	RST	517:519	arg1	V					587:587	Cluster V	579:587	Cluster V (RST 17)	579:596	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16T), Cluster II (RST 9T and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685T (96.3%), Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%), Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%), Bifidobacterium reuteri DSM 23975 T (98.9%) and Bifidobacterium myosotis DSM 100196T (99.3%), respectively.
31585749	4	38	dep	RST	517:519	arg1	Cluster					526:532	Cluster III	526:536	Cluster III (RST 7 and RST 11)	526:555	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16T), Cluster II (RST 9T and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685T (96.3%), Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%), Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%), Bifidobacterium reuteri DSM 23975 T (98.9%) and Bifidobacterium myosotis DSM 100196T (99.3%), respectively.
31585749	4	38	dep	RST	517:519	arg1	Cluster					558:564	Cluster IV	558:567	Cluster IV (RST 19)	558:576	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16T), Cluster II (RST 9T and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685T (96.3%), Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%), Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%), Bifidobacterium reuteri DSM 23975 T (98.9%) and Bifidobacterium myosotis DSM 100196T (99.3%), respectively.
31585749	4	39	theme	rRNA	324:327	arg1	Analysis					308:315	Analysis	308:315	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes	308:391	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16T), Cluster II (RST 9T and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685T (96.3%), Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%), Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%), Bifidobacterium reuteri DSM 23975 T (98.9%) and Bifidobacterium myosotis DSM 100196T (99.3%), respectively.
31585749	9	40	theme	RST	1481:1483	arg1	nov					1475:1477	nov	1475:1477	nov	1475:1477	nov. (RST 16T=BCRC 81138T=NBRC 113380T=DSM 106025T ; RST 8=BCRC 81135=NBRC 113377) and Bifidobacterium rousetti sp.
31585749	9	40	theme	RST	1481:1483	arg1	106025T					1518:1524	RST 16T=BCRC 81138T=NBRC 113380T=DSM 106025T	1481:1524	RST 16T=BCRC 81138T=NBRC 113380T=DSM 106025T ; RST 8=BCRC 81135=NBRC 113377	1481:1555	nov. (RST 16T=BCRC 81138T=NBRC 113380T=DSM 106025T ; RST 8=BCRC 81135=NBRC 113377) and Bifidobacterium rousetti sp.
31585749	4	41	dep	Cluster	526:532	arg1	RST					539:541	RST 7 and RST 11	539:554	RST	539:541	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16T), Cluster II (RST 9T and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685T (96.3%), Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%), Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%), Bifidobacterium reuteri DSM 23975 T (98.9%) and Bifidobacterium myosotis DSM 100196T (99.3%), respectively.
31585749	4	41	dep	Cluster	526:532	arg1	RST					549:551	RST	549:551	RST	549:551	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16T), Cluster II (RST 9T and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685T (96.3%), Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%), Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%), Bifidobacterium reuteri DSM 23975 T (98.9%) and Bifidobacterium myosotis DSM 100196T (99.3%), respectively.
31585749	9	42	theme	81138T=NBRC	1494:1504	arg1	nov					1475:1477	nov	1475:1477	nov	1475:1477	nov. (RST 16T=BCRC 81138T=NBRC 113380T=DSM 106025T ; RST 8=BCRC 81135=NBRC 113377) and Bifidobacterium rousetti sp.
31585749	9	42	theme	81138T=NBRC	1494:1504	arg1	106025T					1518:1524	RST 16T=BCRC 81138T=NBRC 113380T=DSM 106025T	1481:1524	RST 16T=BCRC 81138T=NBRC 113380T=DSM 106025T ; RST 8=BCRC 81135=NBRC 113377	1481:1555	nov. (RST 16T=BCRC 81138T=NBRC 113380T=DSM 106025T ; RST 8=BCRC 81135=NBRC 113377) and Bifidobacterium rousetti sp.
31585749	8	43	from	9T	1375:1376	arg1	Cluster					1381:1387	Cluster II	1381:1390	Cluster II	1381:1390	Phenotypic and genotypic tests demonstrated that strains in Cluster I and RST 9T in Cluster II represent two novel species for which the names Bifidobacterium vespertilionis sp.
31585749	0	44	dep	Characterization	0:15	arg1	Description					81:91	Description	81:91	Characterization of Bifidobacterium species in feaces of the Egyptian fruit bat: Description of B. vespertilionis sp.	0:116	Characterization of Bifidobacterium species in feaces of the Egyptian fruit bat: Description of B. vespertilionis sp.
31585749	4	45	theme	reuteri	790:796	arg1	100201T					748:754	Bifidobacterium tissieri DSM 100201T	719:754	Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%)	719:771	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16T), Cluster II (RST 9T and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685T (96.3%), Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%), Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%), Bifidobacterium reuteri DSM 23975 T (98.9%) and Bifidobacterium myosotis DSM 100196T (99.3%), respectively.
31585749	4	45	theme	reuteri	790:796	arg1	T					808:808	Bifidobacterium reuteri DSM 23975 T	774:808	Bifidobacterium reuteri DSM 23975 T	774:808	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16T), Cluster II (RST 9T and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685T (96.3%), Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%), Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%), Bifidobacterium reuteri DSM 23975 T (98.9%) and Bifidobacterium myosotis DSM 100196T (99.3%), respectively.
31585749	3	46	theme	aegyptiacus	219:229	arg1	faeces					199:204	faeces	199:204	faeces of Rousettus aegyptiacus	199:229	Fifteen bifidobacterial strains were obtained from faeces of Rousettus aegyptiacus; after grouping them by RAPD PCR only eight were selected and characterized.
31585749	4	47	theme	23975	802:806	arg1	100201T					748:754	Bifidobacterium tissieri DSM 100201T	719:754	Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%)	719:771	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16T), Cluster II (RST 9T and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685T (96.3%), Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%), Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%), Bifidobacterium reuteri DSM 23975 T (98.9%) and Bifidobacterium myosotis DSM 100196T (99.3%), respectively.
31585749	4	47	theme	23975	802:806	arg1	T					808:808	Bifidobacterium reuteri DSM 23975 T	774:808	Bifidobacterium reuteri DSM 23975 T	774:808	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16T), Cluster II (RST 9T and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685T (96.3%), Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%), Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%), Bifidobacterium reuteri DSM 23975 T (98.9%) and Bifidobacterium myosotis DSM 100196T (99.3%), respectively.
31585749	5	48	theme	strain	907:912	arg1	RST					914:916	strain RST 9	907:918	strain RST 9	907:918	Strains in Cluster I and strain RST 9 in Cluster II could not be placed within any recognized species while the other ones were identified as known species.
31585749	9	49	theme	81135=NBRC	1539:1548	arg1	113377					1550:1555	RST 8=BCRC 81135=NBRC 113377	1528:1555	RST 16T=BCRC 81138T=NBRC 113380T=DSM 106025T ; RST 8=BCRC 81135=NBRC 113377	1481:1555	nov. (RST 16T=BCRC 81138T=NBRC 113380T=DSM 106025T ; RST 8=BCRC 81135=NBRC 113377) and Bifidobacterium rousetti sp.
31585749	4	50	theme	Bifidobacterium	614:628	arg1	100685T					643:649	Bifidobacterium avesanii DSM 100685T	614:649	Bifidobacterium avesanii DSM 100685T	614:649	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16T), Cluster II (RST 9T and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685T (96.3%), Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%), Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%), Bifidobacterium reuteri DSM 23975 T (98.9%) and Bifidobacterium myosotis DSM 100196T (99.3%), respectively.
31585749	4	51	theme	housekeeping	341:352	arg1	genes					387:391	five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes	336:391	five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes	336:391	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16T), Cluster II (RST 9T and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685T (96.3%), Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%), Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%), Bifidobacterium reuteri DSM 23975 T (98.9%) and Bifidobacterium myosotis DSM 100196T (99.3%), respectively.
31585749	6	52	theme	nucleotide	1051:1060	arg1	lower					1157:1161	lower	1157:1161	lower	1157:1161	The average nucleotide identity values between two novel strains, RST 16T and RST 9T and their closest relatives were lower than 79% and 89%, respectively.
31585749	6	52	theme	nucleotide	1051:1060	arg1	values					1071:1076	The average nucleotide identity values	1039:1076	The average nucleotide identity values between two novel strains, RST 16T and RST 9T	1039:1122	The average nucleotide identity values between two novel strains, RST 16T and RST 9T and their closest relatives were lower than 79% and 89%, respectively.
31585749	4	53	theme	DSM	639:641	arg1	100685T					643:649	Bifidobacterium avesanii DSM 100685T	614:649	Bifidobacterium avesanii DSM 100685T	614:649	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16T), Cluster II (RST 9T and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685T (96.3%), Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%), Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%), Bifidobacterium reuteri DSM 23975 T (98.9%) and Bifidobacterium myosotis DSM 100196T (99.3%), respectively.
31585749	8	54	dep	sp	1471:1472	arg1	names					1434:1438	the names	1430:1438	the names	1430:1438	Phenotypic and genotypic tests demonstrated that strains in Cluster I and RST 9T in Cluster II represent two novel species for which the names Bifidobacterium vespertilionis sp.
31585749	8	55	theme	RST	1371:1373	arg1	9T					1375:1376	RST 9T	1371:1376	RST 9T in Cluster II	1371:1390	Phenotypic and genotypic tests demonstrated that strains in Cluster I and RST 9T in Cluster II represent two novel species for which the names Bifidobacterium vespertilionis sp.
31585749	7	56	theme	42.1	1276:1279	arg1	%					1280:1280	32.5 and 42.1%	1267:1280	%	1280:1280	In silico DNA-DNA hybridization values for those closest relatives were 32.5 and 42.1%, respectively.
31585749	8	57	dep	Bifidobacterium	1440:1454	arg1	vespertilionis					1456:1469	vespertilionis	1456:1469	vespertilionis	1456:1469	Phenotypic and genotypic tests demonstrated that strains in Cluster I and RST 9T in Cluster II represent two novel species for which the names Bifidobacterium vespertilionis sp.
31585749	5	58	from	I	901:901	arg1	Cluster					923:929	Cluster II	923:932	Cluster II	923:932	Strains in Cluster I and strain RST 9 in Cluster II could not be placed within any recognized species while the other ones were identified as known species.
31585749	7	59	theme	32.5	1267:1270	arg1	%					1280:1280	32.5 and 42.1%	1267:1280	%	1280:1280	In silico DNA-DNA hybridization values for those closest relatives were 32.5 and 42.1%, respectively.
31585749	3	60	theme	RAPD	255:258	arg1	PCR					260:262	RAPD PCR	255:262	RAPD PCR	255:262	Fifteen bifidobacterial strains were obtained from faeces of Rousettus aegyptiacus; after grouping them by RAPD PCR only eight were selected and characterized.
31585749	10	61	theme	113378T=DSM	1621:1631	arg1	nov					1591:1593	nov	1591:1593	nov	1591:1593	nov. (RST 9T=BCRC 81136T=NBRC 113378T=DSM 106027T) are proposed.
31585749	10	61	theme	113378T=DSM	1621:1631	arg1	106027T					1633:1639	RST 9T=BCRC 81136T=NBRC 113378T=DSM 106027T	1597:1639	RST 9T=BCRC 81136T=NBRC 113378T=DSM 106027T	1597:1639	nov. (RST 9T=BCRC 81136T=NBRC 113378T=DSM 106027T) are proposed.
31585749	4	62	dep	Bifidobacterium	822:836	arg1	myosotis					838:845	myosotis	838:845	myosotis	838:845	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16T), Cluster II (RST 9T and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685T (96.3%), Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%), Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%), Bifidobacterium reuteri DSM 23975 T (98.9%) and Bifidobacterium myosotis DSM 100196T (99.3%), respectively.
31585749	4	63	dep	hsp60	355:359	arg1	rpoB					362:365	rpoB	362:365	rpoB	362:365	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16T), Cluster II (RST 9T and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685T (96.3%), Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%), Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%), Bifidobacterium reuteri DSM 23975 T (98.9%) and Bifidobacterium myosotis DSM 100196T (99.3%), respectively.
31585749	4	63	dep	hsp60	355:359	arg1	dnaJ					374:377	dnaJ	374:377	dnaJ	374:377	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16T), Cluster II (RST 9T and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685T (96.3%), Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%), Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%), Bifidobacterium reuteri DSM 23975 T (98.9%) and Bifidobacterium myosotis DSM 100196T (99.3%), respectively.
31585749	4	63	dep	hsp60	355:359	arg1	G					384:384	dna G	380:384	dna G	380:384	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16T), Cluster II (RST 9T and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685T (96.3%), Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%), Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%), Bifidobacterium reuteri DSM 23975 T (98.9%) and Bifidobacterium myosotis DSM 100196T (99.3%), respectively.
31585749	4	63	dep	hsp60	355:359	arg1	clpC					368:371	clpC	368:371	clpC	368:371	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16T), Cluster II (RST 9T and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685T (96.3%), Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%), Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%), Bifidobacterium reuteri DSM 23975 T (98.9%) and Bifidobacterium myosotis DSM 100196T (99.3%), respectively.
31585749	10	64	theme	9T=BCRC	1601:1607	arg1	nov					1591:1593	nov	1591:1593	nov	1591:1593	nov. (RST 9T=BCRC 81136T=NBRC 113378T=DSM 106027T) are proposed.
31585749	10	64	theme	9T=BCRC	1601:1607	arg1	106027T					1633:1639	RST 9T=BCRC 81136T=NBRC 113378T=DSM 106027T	1597:1639	RST 9T=BCRC 81136T=NBRC 113378T=DSM 106027T	1597:1639	nov. (RST 9T=BCRC 81136T=NBRC 113378T=DSM 106027T) are proposed.
31585749	4	65	dep	Bifidobacterium	719:733	arg1	tissieri					735:742	tissieri	735:742	tissieri	735:742	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16T), Cluster II (RST 9T and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685T (96.3%), Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%), Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%), Bifidobacterium reuteri DSM 23975 T (98.9%) and Bifidobacterium myosotis DSM 100196T (99.3%), respectively.
31585749	4	66	theme	callitrichos	676:687	arg1	23973T					693:698	Bifidobacterium callitrichos DSM 23973T	660:698	Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%)	660:716	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16T), Cluster II (RST 9T and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685T (96.3%), Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%), Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%), Bifidobacterium reuteri DSM 23975 T (98.9%) and Bifidobacterium myosotis DSM 100196T (99.3%), respectively.
31585749	4	67	theme	dna	380:382	arg1	rpoB					362:365	rpoB	362:365	rpoB	362:365	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16T), Cluster II (RST 9T and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685T (96.3%), Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%), Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%), Bifidobacterium reuteri DSM 23975 T (98.9%) and Bifidobacterium myosotis DSM 100196T (99.3%), respectively.
31585749	4	67	theme	dna	380:382	arg1	G					384:384	dna G	380:384	dna G	380:384	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16T), Cluster II (RST 9T and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685T (96.3%), Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%), Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%), Bifidobacterium reuteri DSM 23975 T (98.9%) and Bifidobacterium myosotis DSM 100196T (99.3%), respectively.
31585749	5	68	theme	recognized	965:974	arg1	species					976:982	any recognized species	961:982	any recognized species	961:982	Strains in Cluster I and strain RST 9 in Cluster II could not be placed within any recognized species while the other ones were identified as known species.
31585749	0	69	theme	fruit	70:74	arg1	bat					76:78	the Egyptian fruit bat	57:78	the Egyptian fruit bat	57:78	Characterization of Bifidobacterium species in feaces of the Egyptian fruit bat: Description of B. vespertilionis sp.
31585749	1	70	theme	B.	127:128	arg1	sp					139:140	B. rousetti sp	127:140	B. rousetti sp	127:140	nov. and B. rousetti sp.
31585749	6	71	theme	identity	1062:1069	arg1	lower					1157:1161	lower	1157:1161	lower	1157:1161	The average nucleotide identity values between two novel strains, RST 16T and RST 9T and their closest relatives were lower than 79% and 89%, respectively.
31585749	6	71	theme	identity	1062:1069	arg1	values					1071:1076	The average nucleotide identity values	1039:1076	The average nucleotide identity values between two novel strains, RST 16T and RST 9T	1039:1122	The average nucleotide identity values between two novel strains, RST 16T and RST 9T and their closest relatives were lower than 79% and 89%, respectively.
31585749	7	72	theme	DNA-DNA	1205:1211	arg1	hybridization					1213:1225	In silico DNA-DNA hybridization	1195:1225	In silico DNA-DNA hybridization values for those closest relatives	1195:1260	In silico DNA-DNA hybridization values for those closest relatives were 32.5 and 42.1%, respectively.
31585749	0	73	theme	sp	114:115	arg1	Description					81:91	Description	81:91	Characterization of Bifidobacterium species in feaces of the Egyptian fruit bat: Description of B. vespertilionis sp.	0:116	Characterization of Bifidobacterium species in feaces of the Egyptian fruit bat: Description of B. vespertilionis sp.
31585749	6	74	theme	average	1043:1049	arg1	lower					1157:1161	lower	1157:1161	lower	1157:1161	The average nucleotide identity values between two novel strains, RST 16T and RST 9T and their closest relatives were lower than 79% and 89%, respectively.
31585749	6	74	theme	average	1043:1049	arg1	values					1071:1076	The average nucleotide identity values	1039:1076	The average nucleotide identity values between two novel strains, RST 16T and RST 9T	1039:1122	The average nucleotide identity values between two novel strains, RST 16T and RST 9T and their closest relatives were lower than 79% and 89%, respectively.
31585749	7	75	theme	In	1195:1196	arg1	hybridization					1213:1225	In silico DNA-DNA hybridization	1195:1225	In silico DNA-DNA hybridization values for those closest relatives	1195:1260	In silico DNA-DNA hybridization values for those closest relatives were 32.5 and 42.1%, respectively.
31585749	0	76	theme	vespertilionis	99:112	arg1	sp					114:115	B. vespertilionis sp	96:115	B. vespertilionis sp	96:115	Characterization of Bifidobacterium species in feaces of the Egyptian fruit bat: Description of B. vespertilionis sp.
31585749	6	77	theme	RST	1117:1119	arg1	strains					1096:1102	two novel strains	1086:1102	two novel strains	1086:1102	The average nucleotide identity values between two novel strains, RST 16T and RST 9T and their closest relatives were lower than 79% and 89%, respectively.
31585749	6	77	theme	RST	1117:1119	arg1	9T					1121:1122	RST 9T	1117:1122	RST 9T	1117:1122	The average nucleotide identity values between two novel strains, RST 16T and RST 9T and their closest relatives were lower than 79% and 89%, respectively.
31585749	0	78	theme	species	36:42	arg1	Characterization					0:15	Characterization	0:15	Characterization of Bifidobacterium species in feaces of the Egyptian fruit bat: Description of B. vespertilionis sp.	0:116	Characterization of Bifidobacterium species in feaces of the Egyptian fruit bat: Description of B. vespertilionis sp.
31585749	5	79	theme	other	994:998	arg1	species					1030:1036	known species	1024:1036	known species	1024:1036	Strains in Cluster I and strain RST 9 in Cluster II could not be placed within any recognized species while the other ones were identified as known species.
31585749	5	79	theme	other	994:998	arg1	ones					1000:1003	the other ones	990:1003	the other ones	990:1003	Strains in Cluster I and strain RST 9 in Cluster II could not be placed within any recognized species while the other ones were identified as known species.
31585749	5	80	theme	Cluster	893:899	arg1	I					901:901	Cluster I	893:901	Cluster I	893:901	Strains in Cluster I and strain RST 9 in Cluster II could not be placed within any recognized species while the other ones were identified as known species.
31585749	8	81	from	I	1365:1365	arg1	Cluster					1381:1387	Cluster II	1381:1390	Cluster II	1381:1390	Phenotypic and genotypic tests demonstrated that strains in Cluster I and RST 9T in Cluster II represent two novel species for which the names Bifidobacterium vespertilionis sp.
31585749	4	82	dep	23973T	693:698	arg1	%					715:715	99.2% and 99.7%	701:715	%	715:715	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16T), Cluster II (RST 9T and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685T (96.3%), Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%), Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%), Bifidobacterium reuteri DSM 23975 T (98.9%) and Bifidobacterium myosotis DSM 100196T (99.3%), respectively.
31585749	4	82	dep	23973T	693:698	arg1	%					705:705	99.2% and 99.7%	701:715	%	705:705	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16T), Cluster II (RST 9T and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685T (96.3%), Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%), Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%), Bifidobacterium reuteri DSM 23975 T (98.9%) and Bifidobacterium myosotis DSM 100196T (99.3%), respectively.
31585749	6	83	theme	novel	1090:1094	arg1	strains					1096:1102	two novel strains	1086:1102	two novel strains	1086:1102	The average nucleotide identity values between two novel strains, RST 16T and RST 9T and their closest relatives were lower than 79% and 89%, respectively.
31585749	6	83	theme	novel	1090:1094	arg1	16T					1109:1111	RST 16T	1105:1111	RST 16T	1105:1111	The average nucleotide identity values between two novel strains, RST 16T and RST 9T and their closest relatives were lower than 79% and 89%, respectively.
31585749	6	83	theme	novel	1090:1094	arg1	9T					1121:1122	RST 9T	1117:1122	RST 9T	1117:1122	The average nucleotide identity values between two novel strains, RST 16T and RST 9T and their closest relatives were lower than 79% and 89%, respectively.
31585749	4	84	theme	16S	320:322	arg1	rRNA					324:327	16S rRNA	320:327	16S rRNA	320:327	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16T), Cluster II (RST 9T and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685T (96.3%), Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%), Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%), Bifidobacterium reuteri DSM 23975 T (98.9%) and Bifidobacterium myosotis DSM 100196T (99.3%), respectively.
31585749	8	85	theme	Phenotypic	1297:1306	arg1	tests					1322:1326	Phenotypic and genotypic tests	1297:1326	Phenotypic and genotypic tests	1297:1326	Phenotypic and genotypic tests demonstrated that strains in Cluster I and RST 9T in Cluster II represent two novel species for which the names Bifidobacterium vespertilionis sp.
31585749	9	86	theme	16T=BCRC	1485:1492	arg1	nov					1475:1477	nov	1475:1477	nov	1475:1477	nov. (RST 16T=BCRC 81138T=NBRC 113380T=DSM 106025T ; RST 8=BCRC 81135=NBRC 113377) and Bifidobacterium rousetti sp.
31585749	9	86	theme	16T=BCRC	1485:1492	arg1	106025T					1518:1524	RST 16T=BCRC 81138T=NBRC 113380T=DSM 106025T	1481:1524	RST 16T=BCRC 81138T=NBRC 113380T=DSM 106025T ; RST 8=BCRC 81135=NBRC 113377	1481:1555	nov. (RST 16T=BCRC 81138T=NBRC 113380T=DSM 106025T ; RST 8=BCRC 81135=NBRC 113377) and Bifidobacterium rousetti sp.
31585749	8	87	theme	genotypic	1312:1320	arg1	tests					1322:1326	Phenotypic and genotypic tests	1297:1326	Phenotypic and genotypic tests	1297:1326	Phenotypic and genotypic tests demonstrated that strains in Cluster I and RST 9T in Cluster II represent two novel species for which the names Bifidobacterium vespertilionis sp.
31585749	9	88	theme	113380T=DSM	1506:1516	arg1	nov					1475:1477	nov	1475:1477	nov	1475:1477	nov. (RST 16T=BCRC 81138T=NBRC 113380T=DSM 106025T ; RST 8=BCRC 81135=NBRC 113377) and Bifidobacterium rousetti sp.
31585749	9	88	theme	113380T=DSM	1506:1516	arg1	106025T					1518:1524	RST 16T=BCRC 81138T=NBRC 113380T=DSM 106025T	1481:1524	RST 16T=BCRC 81138T=NBRC 113380T=DSM 106025T ; RST 8=BCRC 81135=NBRC 113377	1481:1555	nov. (RST 16T=BCRC 81138T=NBRC 113380T=DSM 106025T ; RST 8=BCRC 81135=NBRC 113377) and Bifidobacterium rousetti sp.
31585749	4	89	theme	RST	506:508	arg1	9T					510:511	RST 9T	506:511	RST 9T	506:511	Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16T), Cluster II (RST 9T and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685T (96.3%), Bifidobacterium callitrichos DSM 23973T (99.2% and 99.7%), Bifidobacterium tissieri DSM 100201T (99.7 and 99.2%), Bifidobacterium reuteri DSM 23975 T (98.9%) and Bifidobacterium myosotis DSM 100196T (99.3%), respectively.
31585749	6	90	theme	closest	1134:1140	arg1	relatives					1142:1150	their closest relatives	1128:1150	their closest relatives	1128:1150	The average nucleotide identity values between two novel strains, RST 16T and RST 9T and their closest relatives were lower than 79% and 89%, respectively.
30010523	14	0	theme	genus	1504:1508	arg1	Euzebya					1510:1516	genus Euzebya	1504:1516	genus Euzebya	1504:1516	Based on phylogenetic, phenotypic, chemotaxonomic evidence and genomic analyses, strain DSW09T is a novel species of genus Euzebya, for which the name Euzebya rosea sp.
30010523	5	1	theme	gene	580:583	arg1	similarity					585:594	16S rRNA gene similarity	571:594	16S rRNA gene similarity	571:594	The closest relative was Euzebya tangerina F10T (96.46 % of 16S rRNA gene similarity).
30010523	7	2	theme	fatty	756:760	arg1	acids					762:766	The major cellular fatty acids	737:766	The major cellular fatty acids	737:766	The major cellular fatty acids were summed feature 3 (comprising C16 : 1ω7c and/or C15 : 0iso 2OH), C17 : 1ω8c and C16 : 0.
30010523	5	3	theme	similarity	585:594	arg1	similarity					585:594	16S rRNA gene similarity	571:594	16S rRNA gene similarity	571:594	The closest relative was Euzebya tangerina F10T (96.46 % of 16S rRNA gene similarity).
30010523	5	3	theme	similarity	585:594	arg1	%					566:566	96.46 %	560:566	96.46 % of 16S rRNA gene similarity	560:594	The closest relative was Euzebya tangerina F10T (96.46 % of 16S rRNA gene similarity).
30010523	1	4	theme	genus	124:128	arg1	Euzebya					130:136	the genus Euzebya	120:136	the genus Euzebya	120:136	nov., a rare actinobacterium isolated from the East China Sea and analysis of two genome sequences in the genus Euzebya.
30010523	1	5	from	actinobacterium	31:45	arg1	Euzebya					130:136	the genus Euzebya	120:136	the genus Euzebya	120:136	nov., a rare actinobacterium isolated from the East China Sea and analysis of two genome sequences in the genus Euzebya.
30010523	4	6	dep	aerobic	407:413	arg1	aerobic					407:413	aerobic	407:413	aerobic	407:413	The cells were aerobic, Gram-positive, non-motile, non-spore-forming and rod-shaped (0.4 µm wide and 1.5-4.0 µm long).
30010523	4	6	dep	aerobic	407:413	arg1	cells					396:400	The cells	392:400	The cells	392:400	The cells were aerobic, Gram-positive, non-motile, non-spore-forming and rod-shaped (0.4 µm wide and 1.5-4.0 µm long).
30010523	4	6	dep	aerobic	407:413	arg1	wide					484:487	wide	484:487	wide	484:487	The cells were aerobic, Gram-positive, non-motile, non-spore-forming and rod-shaped (0.4 µm wide and 1.5-4.0 µm long).
30010523	4	6	dep	aerobic	407:413	arg1	long					504:507	long	504:507	long	504:507	The cells were aerobic, Gram-positive, non-motile, non-spore-forming and rod-shaped (0.4 µm wide and 1.5-4.0 µm long).
30010523	12	7	theme	%	1125:1125	arg1	G+C content					1127:1137	a 71.29 mol% G+C content	1114:1137	a 71.29 mol% G+C content for strain DSW09T	1114:1155	The genome size was 5 509 297 bp with a 71.29 mol% G+C content for strain DSW09T, while 4 781 440 bp with a 68.87 mol% G+C content for E. tangerina F10T.
30010523	7	8	theme	major	741:745	arg1	acids					762:766	The major cellular fatty acids	737:766	The major cellular fatty acids	737:766	The major cellular fatty acids were summed feature 3 (comprising C16 : 1ω7c and/or C15 : 0iso 2OH), C17 : 1ω8c and C16 : 0.
30010523	7	9	dep	C16 	802:805	arg1	2OH					831:833	2OH	831:833	2OH	831:833	The major cellular fatty acids were summed feature 3 (comprising C16 : 1ω7c and/or C15 : 0iso 2OH), C17 : 1ω8c and C16 : 0.
30010523	7	9	dep	C16 	802:805	arg1	 1ω7c					807:811	 1ω7c	807:811	 1ω7c	807:811	The major cellular fatty acids were summed feature 3 (comprising C16 : 1ω7c and/or C15 : 0iso 2OH), C17 : 1ω8c and C16 : 0.
30010523	7	9	dep	C16 	802:805	arg1	C15 					820:823	C15 	820:823	C15 	820:823	The major cellular fatty acids were summed feature 3 (comprising C16 : 1ω7c and/or C15 : 0iso 2OH), C17 : 1ω8c and C16 : 0.
30010523	8	10	theme	predominant	865:875	arg1	diphosphatidylglycerol					893:914	diphosphatidylglycerol	893:914	diphosphatidylglycerol	893:914	The predominant polar lipid was diphosphatidylglycerol.
30010523	8	10	theme	predominant	865:875	arg1	lipid					883:887	The predominant polar lipid	861:887	The predominant polar lipid	861:887	The predominant polar lipid was diphosphatidylglycerol.
30010523	1	11	from	sequences	107:115	arg1	Euzebya					130:136	the genus Euzebya	120:136	the genus Euzebya	120:136	nov., a rare actinobacterium isolated from the East China Sea and analysis of two genome sequences in the genus Euzebya.
30010523	2	12	theme	drug	247:250	arg1	development					252:262	drug development	247:262	drug development	247:262	Rare Actinobacteria, known as non-Streptomyces, hold great potential to produce new bioactive compounds for drug development.
30010523	12	13	with	4 781 440 bp	1164:1175	arg1	G+C content					1195:1205	a 68.87 mol% G+C content	1182:1205	a 68.87 mol% G+C content for E. tangerina F10T	1182:1227	The genome size was 5 509 297 bp with a 71.29 mol% G+C content for strain DSW09T, while 4 781 440 bp with a 68.87 mol% G+C content for E. tangerina F10T.
30010523	13	14	theme	digital	1266:1272	arg1	hybridization					1282:1294	digital DNA-DNA hybridization	1266:1294	digital DNA-DNA hybridization	1266:1294	The average nucleotide identity and digital DNA-DNA hybridization values between strain DSW09T and E. tangerina F10T were 73.44 % and 16.43 %, respectively.
30010523	6	15	theme	NaCl	686:689	arg1	concentrations					691:704	NaCl concentrations	686:704	NaCl concentrations of 0.5-5.0 % (w/v; 1.0-4.0 %)	686:734	Cell growth occurred at 15-45 °C (optimum, 25-30 °C), at pH 6.0-9.0 (pH 6.0-7.0) and at NaCl concentrations of 0.5-5.0 % (w/v; 1.0-4.0 %).
30010523	7	16	dep	C17 	837:840	arg1	 0					857:858	 0	857:858	 0	857:858	The major cellular fatty acids were summed feature 3 (comprising C16 : 1ω7c and/or C15 : 0iso 2OH), C17 : 1ω8c and C16 : 0.
30010523	7	16	dep	C17 	837:840	arg1	C16 					852:855	C16 	852:855	C16 	852:855	The major cellular fatty acids were summed feature 3 (comprising C16 : 1ω7c and/or C15 : 0iso 2OH), C17 : 1ω8c and C16 : 0.
30010523	7	16	dep	C17 	837:840	arg1	 1ω8c					842:846	 1ω8c	842:846	 1ω8c	842:846	The major cellular fatty acids were summed feature 3 (comprising C16 : 1ω7c and/or C15 : 0iso 2OH), C17 : 1ω8c and C16 : 0.
30010523	13	17	theme	strain	1311:1316	arg1	DSW09T					1318:1323	strain DSW09T	1311:1323	strain DSW09T	1311:1323	The average nucleotide identity and digital DNA-DNA hybridization values between strain DSW09T and E. tangerina F10T were 73.44 % and 16.43 %, respectively.
30010523	3	18	theme	rare	313:316	arg1	Actinobacteria					318:331	those rare Actinobacteria	307:331	those rare Actinobacteria	307:331	A strain designated DSW09T, which belongs those rare Actinobacteria, was isolated from surface seawater of the East China Sea.
30010523	5	19	theme	closest	515:521	arg1	F10T					554:557	F10T	554:557	F10T	554:557	The closest relative was Euzebya tangerina F10T (96.46 % of 16S rRNA gene similarity).
30010523	5	19	theme	closest	515:521	arg1	relative					523:530	The closest relative	511:530	The closest relative	511:530	The closest relative was Euzebya tangerina F10T (96.46 % of 16S rRNA gene similarity).
30010523	0	20	theme	Euzebya	0:6	arg1	sp					14:15	Euzebya rosea sp	0:15	Euzebya rosea sp.	0:16	Euzebya rosea sp.
30010523	0	21	theme	rosea	8:12	arg1	sp					14:15	Euzebya rosea sp	0:15	Euzebya rosea sp.	0:16	Euzebya rosea sp.
30010523	5	22	theme	16S	571:573	arg1	similarity					585:594	16S rRNA gene similarity	571:594	16S rRNA gene similarity	571:594	The closest relative was Euzebya tangerina F10T (96.46 % of 16S rRNA gene similarity).
30010523	1	23	theme	East	65:68	arg1	Sea					76:78	the East China Sea	61:78	the East China Sea	61:78	nov., a rare actinobacterium isolated from the East China Sea and analysis of two genome sequences in the genus Euzebya.
30010523	14	24	theme	Euzebya	1538:1544	arg1	sp					1552:1553	the name Euzebya rosea sp	1529:1553	the name Euzebya rosea sp	1529:1553	Based on phylogenetic, phenotypic, chemotaxonomic evidence and genomic analyses, strain DSW09T is a novel species of genus Euzebya, for which the name Euzebya rosea sp.
30010523	3	25	theme	China	381:385	arg1	Sea					387:389	the East China Sea	372:389	the East China Sea	372:389	A strain designated DSW09T, which belongs those rare Actinobacteria, was isolated from surface seawater of the East China Sea.
30010523	3	26	theme	surface	352:358	arg1	seawater					360:367	surface seawater	352:367	surface seawater of the East China Sea	352:389	A strain designated DSW09T, which belongs those rare Actinobacteria, was isolated from surface seawater of the East China Sea.
30010523	1	27	attach	isolated	47:54	arg2	actinobacterium					31:45	a rare actinobacterium	24:45	a rare actinobacterium isolated from the East China Sea	24:78	nov., a rare actinobacterium isolated from the East China Sea and analysis of two genome sequences in the genus Euzebya.
30010523	1	27	attach	isolated	47:54	arg1	Sea					76:78	the East China Sea	61:78	the East China Sea	61:78	nov., a rare actinobacterium isolated from the East China Sea and analysis of two genome sequences in the genus Euzebya.
30010523	5	28	theme	rRNA	575:578	arg1	similarity					585:594	16S rRNA gene similarity	571:594	16S rRNA gene similarity	571:594	The closest relative was Euzebya tangerina F10T (96.46 % of 16S rRNA gene similarity).
30010523	1	29	from	analysis	84:91	arg1	Euzebya					130:136	the genus Euzebya	120:136	the genus Euzebya	120:136	nov., a rare actinobacterium isolated from the East China Sea and analysis of two genome sequences in the genus Euzebya.
30010523	1	30	theme	China	70:74	arg1	Sea					76:78	the East China Sea	61:78	the East China Sea	61:78	nov., a rare actinobacterium isolated from the East China Sea and analysis of two genome sequences in the genus Euzebya.
30010523	3	31	theme	Sea	387:389	arg1	seawater					360:367	surface seawater	352:367	surface seawater of the East China Sea	352:389	A strain designated DSW09T, which belongs those rare Actinobacteria, was isolated from surface seawater of the East China Sea.
30010523	6	32	dep	%	717:717	arg1	w/v					720:722	w/v	720:722	w/v; 1.0-4.0 %	720:733	Cell growth occurred at 15-45 °C (optimum, 25-30 °C), at pH 6.0-9.0 (pH 6.0-7.0) and at NaCl concentrations of 0.5-5.0 % (w/v; 1.0-4.0 %).
30010523	16	33	theme	=DMS	1601:1604	arg1	1K03290T					1619:1626	=DMS 104446T=MCCC 1K03290T	1601:1626	=DMS 104446T=MCCC 1K03290T	1601:1626	The type strain is DSW09T (=DMS 104446T=MCCC 1K03290T).
30010523	16	33	theme	=DMS	1601:1604	arg1	DSW09T					1593:1598	DSW09T	1593:1598	DSW09T (=DMS 104446T=MCCC 1K03290T)	1593:1627	The type strain is DSW09T (=DMS 104446T=MCCC 1K03290T).
30010523	6	34	dep	optimum	632:638	arg1	25-30 °C					641:648	25-30 °C	641:648	25-30 °C	641:648	Cell growth occurred at 15-45 °C (optimum, 25-30 °C), at pH 6.0-9.0 (pH 6.0-7.0) and at NaCl concentrations of 0.5-5.0 % (w/v; 1.0-4.0 %).
30010523	12	35	theme	%	1193:1193	arg1	G+C content					1195:1205	a 68.87 mol% G+C content	1182:1205	a 68.87 mol% G+C content for E. tangerina F10T	1182:1227	The genome size was 5 509 297 bp with a 71.29 mol% G+C content for strain DSW09T, while 4 781 440 bp with a 68.87 mol% G+C content for E. tangerina F10T.
30010523	9	36	theme	predominant	921:931	arg1	MK-9					949:952	MK-9	949:952	MK-9(H4)	949:956	The predominant menaquinone was MK-9(H4).
30010523	9	36	theme	predominant	921:931	arg1	menaquinone					933:943	The predominant menaquinone	917:943	The predominant menaquinone	917:943	The predominant menaquinone was MK-9(H4).
30010523	12	37	theme	genome	1080:1085	arg1	size					1087:1090	The genome size	1076:1090	The genome size	1076:1090	The genome size was 5 509 297 bp with a 71.29 mol% G+C content for strain DSW09T, while 4 781 440 bp with a 68.87 mol% G+C content for E. tangerina F10T.
30010523	12	37	theme	genome	1080:1085	arg1	5 509 297 bp					1096:1107	5 509 297 bp	1096:1107	5 509 297 bp	1096:1107	The genome size was 5 509 297 bp with a 71.29 mol% G+C content for strain DSW09T, while 4 781 440 bp with a 68.87 mol% G+C content for E. tangerina F10T.
30010523	14	38	theme	Euzebya	1510:1516	arg1	DSW09T					1475:1480	strain DSW09T	1468:1480	strain DSW09T	1468:1480	Based on phylogenetic, phenotypic, chemotaxonomic evidence and genomic analyses, strain DSW09T is a novel species of genus Euzebya, for which the name Euzebya rosea sp.
30010523	14	38	theme	Euzebya	1510:1516	arg1	species					1493:1499	a novel species	1485:1499	a novel species	1485:1499	Based on phylogenetic, phenotypic, chemotaxonomic evidence and genomic analyses, strain DSW09T is a novel species of genus Euzebya, for which the name Euzebya rosea sp.
30010523	12	39	theme	71.29 mol	1116:1124	arg1	G+C content					1127:1137	a 71.29 mol% G+C content	1114:1137	a 71.29 mol% G+C content for strain DSW09T	1114:1155	The genome size was 5 509 297 bp with a 71.29 mol% G+C content for strain DSW09T, while 4 781 440 bp with a 68.87 mol% G+C content for E. tangerina F10T.
30010523	13	40	theme	hybridization	1282:1294	arg1	values					1296:1301	The average nucleotide identity and digital DNA-DNA hybridization values	1230:1301	The average nucleotide identity and digital DNA-DNA hybridization values between strain DSW09T and E. tangerina F10T	1230:1345	The average nucleotide identity and digital DNA-DNA hybridization values between strain DSW09T and E. tangerina F10T were 73.44 % and 16.43 %, respectively.
30010523	13	40	theme	hybridization	1282:1294	arg1	%					1358:1358	73.44 %	1352:1358	73.44 %	1352:1358	The average nucleotide identity and digital DNA-DNA hybridization values between strain DSW09T and E. tangerina F10T were 73.44 % and 16.43 %, respectively.
30010523	6	41	dep	w/v	720:722	arg1	%					733:733	1.0-4.0 %	725:733	w/v; 1.0-4.0 %	720:733	Cell growth occurred at 15-45 °C (optimum, 25-30 °C), at pH 6.0-9.0 (pH 6.0-7.0) and at NaCl concentrations of 0.5-5.0 % (w/v; 1.0-4.0 %).
30010523	11	42	theme	major	1027:1031	arg1	sugars					1043:1048	The major cell-wall sugars	1023:1048	The major cell-wall sugars	1023:1048	The major cell-wall sugars were rhamnose and ribose.
30010523	11	42	theme	major	1027:1031	arg1	rhamnose					1055:1062	rhamnose	1055:1062	rhamnose	1055:1062	The major cell-wall sugars were rhamnose and ribose.
30010523	2	43	theme	Rare	139:142	arg1	Actinobacteria					144:157	Rare Actinobacteria	139:157	Rare Actinobacteria	139:157	Rare Actinobacteria, known as non-Streptomyces, hold great potential to produce new bioactive compounds for drug development.
30010523	6	44	theme	Cell	598:601	arg1	growth					603:608	Cell growth	598:608	Cell growth	598:608	Cell growth occurred at 15-45 °C (optimum, 25-30 °C), at pH 6.0-9.0 (pH 6.0-7.0) and at NaCl concentrations of 0.5-5.0 % (w/v; 1.0-4.0 %).
30010523	3	45	theme	East	376:379	arg1	Sea					387:389	the East China Sea	372:389	the East China Sea	372:389	A strain designated DSW09T, which belongs those rare Actinobacteria, was isolated from surface seawater of the East China Sea.
30010523	11	46	theme	cell-wall	1033:1041	arg1	sugars					1043:1048	The major cell-wall sugars	1023:1048	The major cell-wall sugars	1023:1048	The major cell-wall sugars were rhamnose and ribose.
30010523	11	46	theme	cell-wall	1033:1041	arg1	rhamnose					1055:1062	rhamnose	1055:1062	rhamnose	1055:1062	The major cell-wall sugars were rhamnose and ribose.
30010523	2	47	theme	great	192:196	arg1	potential					198:206	great potential	192:206	great potential to produce new bioactive compounds for drug development	192:262	Rare Actinobacteria, known as non-Streptomyces, hold great potential to produce new bioactive compounds for drug development.
30010523	14	48	theme	phenotypic	1410:1419	arg1	evidence					1437:1444	phenotypic, chemotaxonomic evidence	1410:1444	phenotypic, chemotaxonomic evidence	1410:1444	Based on phylogenetic, phenotypic, chemotaxonomic evidence and genomic analyses, strain DSW09T is a novel species of genus Euzebya, for which the name Euzebya rosea sp.
30010523	10	49	theme	cell-wall	963:971	arg1	γ-type					994:999	A1 γ-type	991:999	A1 γ-type	991:999	The cell-wall peptidoglycan was A1 γ-type, containing meso-DPA.
30010523	10	49	theme	cell-wall	963:971	arg1	peptidoglycan					973:985	The cell-wall peptidoglycan	959:985	The cell-wall peptidoglycan	959:985	The cell-wall peptidoglycan was A1 γ-type, containing meso-DPA.
30010523	13	50	theme	nucleotide	1242:1251	arg1	identity					1253:1260	average nucleotide identity	1234:1260	average nucleotide identity	1234:1260	The average nucleotide identity and digital DNA-DNA hybridization values between strain DSW09T and E. tangerina F10T were 73.44 % and 16.43 %, respectively.
30010523	14	51	theme	genomic	1450:1456	arg1	analyses					1458:1465	genomic analyses	1450:1465	genomic analyses	1450:1465	Based on phylogenetic, phenotypic, chemotaxonomic evidence and genomic analyses, strain DSW09T is a novel species of genus Euzebya, for which the name Euzebya rosea sp.
30010523	14	52	theme	novel	1487:1491	arg1	DSW09T					1475:1480	strain DSW09T	1468:1480	strain DSW09T	1468:1480	Based on phylogenetic, phenotypic, chemotaxonomic evidence and genomic analyses, strain DSW09T is a novel species of genus Euzebya, for which the name Euzebya rosea sp.
30010523	14	52	theme	novel	1487:1491	arg1	species					1493:1499	a novel species	1485:1499	a novel species	1485:1499	Based on phylogenetic, phenotypic, chemotaxonomic evidence and genomic analyses, strain DSW09T is a novel species of genus Euzebya, for which the name Euzebya rosea sp.
30010523	13	53	theme	average	1234:1240	arg1	identity					1253:1260	average nucleotide identity	1234:1260	average nucleotide identity	1234:1260	The average nucleotide identity and digital DNA-DNA hybridization values between strain DSW09T and E. tangerina F10T were 73.44 % and 16.43 %, respectively.
30010523	13	54	theme	identity	1253:1260	arg1	values					1296:1301	The average nucleotide identity and digital DNA-DNA hybridization values	1230:1301	The average nucleotide identity and digital DNA-DNA hybridization values between strain DSW09T and E. tangerina F10T	1230:1345	The average nucleotide identity and digital DNA-DNA hybridization values between strain DSW09T and E. tangerina F10T were 73.44 % and 16.43 %, respectively.
30010523	13	54	theme	identity	1253:1260	arg1	%					1358:1358	73.44 %	1352:1358	73.44 %	1352:1358	The average nucleotide identity and digital DNA-DNA hybridization values between strain DSW09T and E. tangerina F10T were 73.44 % and 16.43 %, respectively.
30010523	8	55	theme	polar	877:881	arg1	diphosphatidylglycerol					893:914	diphosphatidylglycerol	893:914	diphosphatidylglycerol	893:914	The predominant polar lipid was diphosphatidylglycerol.
30010523	8	55	theme	polar	877:881	arg1	lipid					883:887	The predominant polar lipid	861:887	The predominant polar lipid	861:887	The predominant polar lipid was diphosphatidylglycerol.
30010523	16	56	theme	type	1578:1581	arg1	DSW09T					1593:1598	DSW09T	1593:1598	DSW09T (=DMS 104446T=MCCC 1K03290T)	1593:1627	The type strain is DSW09T (=DMS 104446T=MCCC 1K03290T).
30010523	16	56	theme	type	1578:1581	arg1	strain					1583:1588	The type strain	1574:1588	The type strain	1574:1588	The type strain is DSW09T (=DMS 104446T=MCCC 1K03290T).
30010523	10	57	contain	containing	1002:1011	arg1	γ-type					994:999	A1 γ-type	991:999	A1 γ-type	991:999	The cell-wall peptidoglycan was A1 γ-type, containing meso-DPA.
30010523	10	57	contain	containing	1002:1011	arg2	meso-DPA					1013:1020	meso-DPA	1013:1020	meso-DPA	1013:1020	The cell-wall peptidoglycan was A1 γ-type, containing meso-DPA.
30010523	10	57	contain	containing	1002:1011	arg1	peptidoglycan					973:985	The cell-wall peptidoglycan	959:985	The cell-wall peptidoglycan	959:985	The cell-wall peptidoglycan was A1 γ-type, containing meso-DPA.
30010523	2	58	theme	bioactive	223:231	arg1	compounds					233:241	new bioactive compounds	219:241	new bioactive compounds	219:241	Rare Actinobacteria, known as non-Streptomyces, hold great potential to produce new bioactive compounds for drug development.
30010523	12	59	with	5 509 297 bp	1096:1107	arg1	G+C content					1127:1137	a 71.29 mol% G+C content	1114:1137	a 71.29 mol% G+C content for strain DSW09T	1114:1155	The genome size was 5 509 297 bp with a 71.29 mol% G+C content for strain DSW09T, while 4 781 440 bp with a 68.87 mol% G+C content for E. tangerina F10T.
30010523	1	60	dep	actinobacterium	31:45	arg1	nov.					18:21	nov.	18:21	nov.	18:21	nov., a rare actinobacterium isolated from the East China Sea and analysis of two genome sequences in the genus Euzebya.
30010523	14	61	dep	phenotypic	1410:1419	arg1	chemotaxonomic					1422:1435	chemotaxonomic	1422:1435	chemotaxonomic	1422:1435	Based on phylogenetic, phenotypic, chemotaxonomic evidence and genomic analyses, strain DSW09T is a novel species of genus Euzebya, for which the name Euzebya rosea sp.
30010523	12	62	theme	68.87 mol	1184:1192	arg1	G+C content					1195:1205	a 68.87 mol% G+C content	1182:1205	a 68.87 mol% G+C content for E. tangerina F10T	1182:1227	The genome size was 5 509 297 bp with a 71.29 mol% G+C content for strain DSW09T, while 4 781 440 bp with a 68.87 mol% G+C content for E. tangerina F10T.
30010523	3	63	attach	isolated	338:345	arg2	strain					267:272	A strain	265:272	A strain	265:272	A strain designated DSW09T, which belongs those rare Actinobacteria, was isolated from surface seawater of the East China Sea.
30010523	3	63	attach	isolated	338:345	arg1	seawater					360:367	surface seawater	352:367	surface seawater of the East China Sea	352:389	A strain designated DSW09T, which belongs those rare Actinobacteria, was isolated from surface seawater of the East China Sea.
30010523	2	64	theme	new	219:221	arg1	compounds					233:241	new bioactive compounds	219:241	new bioactive compounds	219:241	Rare Actinobacteria, known as non-Streptomyces, hold great potential to produce new bioactive compounds for drug development.
30010523	1	65	theme	genome	100:105	arg1	sequences					107:115	two genome sequences	96:115	two genome sequences in the genus Euzebya	96:136	nov., a rare actinobacterium isolated from the East China Sea and analysis of two genome sequences in the genus Euzebya.
30010523	14	66	theme	rosea	1546:1550	arg1	sp					1552:1553	the name Euzebya rosea sp	1529:1553	the name Euzebya rosea sp	1529:1553	Based on phylogenetic, phenotypic, chemotaxonomic evidence and genomic analyses, strain DSW09T is a novel species of genus Euzebya, for which the name Euzebya rosea sp.
30010523	6	67	theme	%	717:717	arg1	concentrations					691:704	NaCl concentrations	686:704	NaCl concentrations of 0.5-5.0 % (w/v; 1.0-4.0 %)	686:734	Cell growth occurred at 15-45 °C (optimum, 25-30 °C), at pH 6.0-9.0 (pH 6.0-7.0) and at NaCl concentrations of 0.5-5.0 % (w/v; 1.0-4.0 %).
30010523	12	68	theme	strain	1143:1148	arg1	DSW09T					1150:1155	strain DSW09T	1143:1155	strain DSW09T	1143:1155	The genome size was 5 509 297 bp with a 71.29 mol% G+C content for strain DSW09T, while 4 781 440 bp with a 68.87 mol% G+C content for E. tangerina F10T.
30010523	13	69	theme	DNA-DNA	1274:1280	arg1	hybridization					1282:1294	digital DNA-DNA hybridization	1266:1294	digital DNA-DNA hybridization	1266:1294	The average nucleotide identity and digital DNA-DNA hybridization values between strain DSW09T and E. tangerina F10T were 73.44 % and 16.43 %, respectively.
30010523	1	70	theme	sequences	107:115	arg1	actinobacterium					31:45	a rare actinobacterium	24:45	a rare actinobacterium isolated from the East China Sea	24:78	nov., a rare actinobacterium isolated from the East China Sea and analysis of two genome sequences in the genus Euzebya.
30010523	1	70	theme	sequences	107:115	arg1	analysis					84:91	analysis	84:91	analysis of two genome sequences in the genus Euzebya	84:136	nov., a rare actinobacterium isolated from the East China Sea and analysis of two genome sequences in the genus Euzebya.
30010523	5	71	dep	F10T	554:557	arg1	similarity					585:594	16S rRNA gene similarity	571:594	16S rRNA gene similarity	571:594	The closest relative was Euzebya tangerina F10T (96.46 % of 16S rRNA gene similarity).
30010523	5	71	dep	F10T	554:557	arg1	%					566:566	96.46 %	560:566	96.46 % of 16S rRNA gene similarity	560:594	The closest relative was Euzebya tangerina F10T (96.46 % of 16S rRNA gene similarity).
30010523	10	72	theme	A1	991:992	arg1	γ-type					994:999	A1 γ-type	991:999	A1 γ-type	991:999	The cell-wall peptidoglycan was A1 γ-type, containing meso-DPA.
30010523	10	72	theme	A1	991:992	arg1	peptidoglycan					973:985	The cell-wall peptidoglycan	959:985	The cell-wall peptidoglycan	959:985	The cell-wall peptidoglycan was A1 γ-type, containing meso-DPA.
30010523	14	73	theme	strain	1468:1473	arg1	DSW09T					1475:1480	strain DSW09T	1468:1480	strain DSW09T	1468:1480	Based on phylogenetic, phenotypic, chemotaxonomic evidence and genomic analyses, strain DSW09T is a novel species of genus Euzebya, for which the name Euzebya rosea sp.
30010523	14	73	theme	strain	1468:1473	arg1	species					1493:1499	a novel species	1485:1499	a novel species	1485:1499	Based on phylogenetic, phenotypic, chemotaxonomic evidence and genomic analyses, strain DSW09T is a novel species of genus Euzebya, for which the name Euzebya rosea sp.
30010523	1	74	theme	rare	26:29	arg1	actinobacterium					31:45	a rare actinobacterium	24:45	a rare actinobacterium isolated from the East China Sea	24:78	nov., a rare actinobacterium isolated from the East China Sea and analysis of two genome sequences in the genus Euzebya.
30010523	1	75	from	Euzebya	130:136	arg1	actinobacterium					31:45	a rare actinobacterium	24:45	a rare actinobacterium isolated from the East China Sea	24:78	nov., a rare actinobacterium isolated from the East China Sea and analysis of two genome sequences in the genus Euzebya.
30010523	1	75	from	Euzebya	130:136	arg1	analysis					84:91	analysis	84:91	analysis of two genome sequences in the genus Euzebya	84:136	nov., a rare actinobacterium isolated from the East China Sea and analysis of two genome sequences in the genus Euzebya.
30010523	16	76	theme	104446T=MCCC	1606:1617	arg1	1K03290T					1619:1626	=DMS 104446T=MCCC 1K03290T	1601:1626	=DMS 104446T=MCCC 1K03290T	1601:1626	The type strain is DSW09T (=DMS 104446T=MCCC 1K03290T).
30010523	16	76	theme	104446T=MCCC	1606:1617	arg1	DSW09T					1593:1598	DSW09T	1593:1598	DSW09T (=DMS 104446T=MCCC 1K03290T)	1593:1627	The type strain is DSW09T (=DMS 104446T=MCCC 1K03290T).
30010523	7	77	theme	cellular	747:754	arg1	acids					762:766	The major cellular fatty acids	737:766	The major cellular fatty acids	737:766	The major cellular fatty acids were summed feature 3 (comprising C16 : 1ω7c and/or C15 : 0iso 2OH), C17 : 1ω8c and C16 : 0.
30010523	14	78	theme	name	1533:1536	arg1	sp					1552:1553	the name Euzebya rosea sp	1529:1553	the name Euzebya rosea sp	1529:1553	Based on phylogenetic, phenotypic, chemotaxonomic evidence and genomic analyses, strain DSW09T is a novel species of genus Euzebya, for which the name Euzebya rosea sp.
30960322	0	0	theme	Gastroresistant	92:106	arg1	Capsules					108:115	Soft Gastroresistant Capsules	87:115	Soft Gastroresistant Capsules	87:115	Gelatin Films Modified with Acidic and Polyelectrolyte Polymers-Material Selection for Soft Gastroresistant Capsules.
30960322	4	1	theme	obtained	573:580	arg1	compositions					587:598	The obtained film compositions	569:598	The obtained film compositions	569:598	The obtained film compositions were subjected to, e.g., disintegration tests, adhesiveness tests, differential scanning calorimetry (DSC), and a transparency study.
30960322	1	2	theme	gelatin-based	188:200	arg1	films					202:206	acid-resistant gelatin-based films	173:206	acid-resistant gelatin-based films	173:206	The following investigation comprised the formation of acid-resistant gelatin-based films, intended for future use in soft-capsule technology.
30960322	0	3	theme	Soft	87:90	arg1	Capsules					108:115	Soft Gastroresistant Capsules	87:115	Soft Gastroresistant Capsules	87:115	Gelatin Films Modified with Acidic and Polyelectrolyte Polymers-Material Selection for Soft Gastroresistant Capsules.
30960322	1	4	theme	films	202:206	arg1	formation					160:168	the formation	156:168	the formation of acid-resistant gelatin-based films, intended for future use in soft-capsule technology	156:258	The following investigation comprised the formation of acid-resistant gelatin-based films, intended for future use in soft-capsule technology.
30960322	4	5	theme	adhesiveness	647:658	arg1	tests					660:664	adhesiveness tests	647:664	adhesiveness tests	647:664	The obtained film compositions were subjected to, e.g., disintegration tests, adhesiveness tests, differential scanning calorimetry (DSC), and a transparency study.
30960322	5	6	theme	commercial	775:784	arg1	polymers					794:801	some commercial enteric polymers	770:801	some commercial enteric polymers (acrylates)	770:813	As a result of the performed study, some commercial enteric polymers (acrylates), as well as cellulose acetate phthalate, were selected as components that have the ability to coalesce and form a continuous phase within a gelatin film.
30960322	5	6	theme	commercial	775:784	arg1	phthalate					845:853	cellulose acetate phthalate	827:853	cellulose acetate phthalate	827:853	As a result of the performed study, some commercial enteric polymers (acrylates), as well as cellulose acetate phthalate, were selected as components that have the ability to coalesce and form a continuous phase within a gelatin film.
30960322	5	6	theme	commercial	775:784	arg1	result					739:744	a result	737:744	a result of the performed study	737:767	As a result of the performed study, some commercial enteric polymers (acrylates), as well as cellulose acetate phthalate, were selected as components that have the ability to coalesce and form a continuous phase within a gelatin film.
30960322	5	6	theme	commercial	775:784	arg1	acrylates					804:812	acrylates	804:812	acrylates	804:812	As a result of the performed study, some commercial enteric polymers (acrylates), as well as cellulose acetate phthalate, were selected as components that have the ability to coalesce and form a continuous phase within a gelatin film.
30960322	5	6	theme	commercial	775:784	arg1	components					873:882	components	873:882	components that have the ability to coalesce and form a continuous phase within a gelatin film	873:966	As a result of the performed study, some commercial enteric polymers (acrylates), as well as cellulose acetate phthalate, were selected as components that have the ability to coalesce and form a continuous phase within a gelatin film.
30960322	2	7	theme	acid-insoluble	331:344	arg1	polymers					346:353	acid-insoluble polymers	331:353	acid-insoluble polymers	331:353	Such film compositions were obtained by including nonionized forms of acid-insoluble polymers in a gelatin-based film-forming mixture.
30960322	2	8	theme	polymers	346:353	arg1	forms					322:326	nonionized forms	311:326	nonionized forms of acid-insoluble polymers	311:353	Such film compositions were obtained by including nonionized forms of acid-insoluble polymers in a gelatin-based film-forming mixture.
30960322	4	9	theme	differential	667:678	arg1	calorimetry					689:699	differential scanning calorimetry	667:699	differential scanning calorimetry (DSC)	667:705	The obtained film compositions were subjected to, e.g., disintegration tests, adhesiveness tests, differential scanning calorimetry (DSC), and a transparency study.
30960322	4	9	theme	differential	667:678	arg1	DSC					702:704	DSC	702:704	DSC	702:704	The obtained film compositions were subjected to, e.g., disintegration tests, adhesiveness tests, differential scanning calorimetry (DSC), and a transparency study.
30960322	3	10	mod	modified	433:440	arg1	films					409:413	The selected films	396:413	The selected films	396:413	The selected films were additionally modified with small amounts of anionic polysaccharides that have potential to interact with gelatin, forming polyelectrolyte complexes.
30960322	3	10	mod	modified	433:440	arg3	amounts					453:459	small amounts	447:459	small amounts of anionic polysaccharides that have potential to interact with gelatin, forming polyelectrolyte complexes	447:566	The selected films were additionally modified with small amounts of anionic polysaccharides that have potential to interact with gelatin, forming polyelectrolyte complexes.
30960322	3	10	mod	modified	433:440	arg3	polysaccharides					472:486	anionic polysaccharides	464:486	anionic polysaccharides that have potential to interact with gelatin, forming polyelectrolyte complexes	464:566	The selected films were additionally modified with small amounts of anionic polysaccharides that have potential to interact with gelatin, forming polyelectrolyte complexes.
30960322	6	11	theme	obtained	1105:1112	arg1	films					1114:1118	the obtained films	1101:1118	the obtained films	1101:1118	The use of a small amount (1.5%) of additional gelling polymers improved the rheological characteristics and adhesive properties of the obtained films, with ί-carrageenan and gellan gum appearing to be the most beneficial.
30960322	5	12	theme	gelatin	955:961	arg1	film					963:966	a gelatin film	953:966	a gelatin film	953:966	As a result of the performed study, some commercial enteric polymers (acrylates), as well as cellulose acetate phthalate, were selected as components that have the ability to coalesce and form a continuous phase within a gelatin film.
30960322	3	13	theme	selected	400:407	arg1	films					409:413	The selected films	396:413	The selected films	396:413	The selected films were additionally modified with small amounts of anionic polysaccharides that have potential to interact with gelatin, forming polyelectrolyte complexes.
30960322	4	14	dep	tests	640:644	arg1	e.g.					619:622	e.g.	619:622	e.g.	619:622	The obtained film compositions were subjected to, e.g., disintegration tests, adhesiveness tests, differential scanning calorimetry (DSC), and a transparency study.
30960322	6	15	dep	characteristics	1058:1072	arg1	the					1042:1044	the	1042:1044	the	1042:1044	The use of a small amount (1.5%) of additional gelling polymers improved the rheological characteristics and adhesive properties of the obtained films, with ί-carrageenan and gellan gum appearing to be the most beneficial.
30960322	3	16	theme	anionic	464:470	arg1	polysaccharides					472:486	anionic polysaccharides	464:486	anionic polysaccharides that have potential to interact with gelatin, forming polyelectrolyte complexes	464:566	The selected films were additionally modified with small amounts of anionic polysaccharides that have potential to interact with gelatin, forming polyelectrolyte complexes.
30960322	5	17	theme	continuous	929:938	arg1	phase					940:944	a continuous phase	927:944	a continuous phase within a gelatin film	927:966	As a result of the performed study, some commercial enteric polymers (acrylates), as well as cellulose acetate phthalate, were selected as components that have the ability to coalesce and form a continuous phase within a gelatin film.
30960322	5	18	contain	have	889:892	arg1	polymers					794:801	some commercial enteric polymers	770:801	some commercial enteric polymers (acrylates)	770:813	As a result of the performed study, some commercial enteric polymers (acrylates), as well as cellulose acetate phthalate, were selected as components that have the ability to coalesce and form a continuous phase within a gelatin film.
30960322	5	18	contain	have	889:892	arg2	ability					898:904	the ability to coalesce and form a continuous phase within a gelatin film	894:966	the ability to coalesce and form a continuous phase within a gelatin film	894:966	As a result of the performed study, some commercial enteric polymers (acrylates), as well as cellulose acetate phthalate, were selected as components that have the ability to coalesce and form a continuous phase within a gelatin film.
30960322	5	18	contain	have	889:892	arg1	components					873:882	components	873:882	components that have the ability to coalesce and form a continuous phase within a gelatin film	873:966	As a result of the performed study, some commercial enteric polymers (acrylates), as well as cellulose acetate phthalate, were selected as components that have the ability to coalesce and form a continuous phase within a gelatin film.
30960322	5	18	contain	have	889:892	arg1	phthalate					845:853	cellulose acetate phthalate	827:853	cellulose acetate phthalate	827:853	As a result of the performed study, some commercial enteric polymers (acrylates), as well as cellulose acetate phthalate, were selected as components that have the ability to coalesce and form a continuous phase within a gelatin film.
30960322	2	19	theme	nonionized	311:320	arg1	forms					322:326	nonionized forms	311:326	nonionized forms of acid-insoluble polymers	311:353	Such film compositions were obtained by including nonionized forms of acid-insoluble polymers in a gelatin-based film-forming mixture.
30960322	4	20	theme	transparency	714:725	arg1	study					727:731	a transparency study	712:731	a transparency study	712:731	The obtained film compositions were subjected to, e.g., disintegration tests, adhesiveness tests, differential scanning calorimetry (DSC), and a transparency study.
30960322	3	21	theme	small	447:451	arg1	amounts					453:459	small amounts	447:459	small amounts of anionic polysaccharides that have potential to interact with gelatin, forming polyelectrolyte complexes	447:566	The selected films were additionally modified with small amounts of anionic polysaccharides that have potential to interact with gelatin, forming polyelectrolyte complexes.
30960322	3	21	theme	small	447:451	arg1	polysaccharides					472:486	anionic polysaccharides	464:486	anionic polysaccharides that have potential to interact with gelatin, forming polyelectrolyte complexes	464:566	The selected films were additionally modified with small amounts of anionic polysaccharides that have potential to interact with gelatin, forming polyelectrolyte complexes.
30960322	6	22	theme	amount	988:993	arg1	use					973:975	The use	969:975	The use of a small amount (1.5%) of additional gelling polymers	969:1031	The use of a small amount (1.5%) of additional gelling polymers improved the rheological characteristics and adhesive properties of the obtained films, with ί-carrageenan and gellan gum appearing to be the most beneficial.
30960322	2	23	theme	gelatin-based	360:372	arg1	mixture					387:393	a gelatin-based film-forming mixture	358:393	a gelatin-based film-forming mixture	358:393	Such film compositions were obtained by including nonionized forms of acid-insoluble polymers in a gelatin-based film-forming mixture.
30960322	6	24	theme	adhesive	1078:1085	arg1	properties					1087:1096	adhesive properties	1078:1096	adhesive properties	1078:1096	The use of a small amount (1.5%) of additional gelling polymers improved the rheological characteristics and adhesive properties of the obtained films, with ί-carrageenan and gellan gum appearing to be the most beneficial.
30960322	0	25	theme	Gelatin	0:6	arg1	Films					8:12	Gelatin Films	0:12	Gelatin Films	0:12	Gelatin Films Modified with Acidic and Polyelectrolyte Polymers-Material Selection for Soft Gastroresistant Capsules.
30960322	4	26	theme	disintegration	625:638	arg1	tests					640:644	disintegration tests	625:644	disintegration tests	625:644	The obtained film compositions were subjected to, e.g., disintegration tests, adhesiveness tests, differential scanning calorimetry (DSC), and a transparency study.
30960322	1	27	theme	future	222:227	arg1	use					229:231	future use	222:231	future use in soft-capsule technology	222:258	The following investigation comprised the formation of acid-resistant gelatin-based films, intended for future use in soft-capsule technology.
30960322	6	28	theme	small	982:986	arg1	%					999:999	1.5%	996:999	1.5%	996:999	The use of a small amount (1.5%) of additional gelling polymers improved the rheological characteristics and adhesive properties of the obtained films, with ί-carrageenan and gellan gum appearing to be the most beneficial.
30960322	6	28	theme	small	982:986	arg1	polymers					1024:1031	additional gelling polymers	1005:1031	additional gelling polymers	1005:1031	The use of a small amount (1.5%) of additional gelling polymers improved the rheological characteristics and adhesive properties of the obtained films, with ί-carrageenan and gellan gum appearing to be the most beneficial.
30960322	6	28	theme	small	982:986	arg1	amount					988:993	a small amount	980:993	a small amount (1.5%) of additional gelling polymers	980:1031	The use of a small amount (1.5%) of additional gelling polymers improved the rheological characteristics and adhesive properties of the obtained films, with ί-carrageenan and gellan gum appearing to be the most beneficial.
30960322	5	29	theme	performed	753:761	arg1	study					763:767	the performed study	749:767	the performed study	749:767	As a result of the performed study, some commercial enteric polymers (acrylates), as well as cellulose acetate phthalate, were selected as components that have the ability to coalesce and form a continuous phase within a gelatin film.
30960322	5	30	theme	study	763:767	arg1	polymers					794:801	some commercial enteric polymers	770:801	some commercial enteric polymers (acrylates)	770:813	As a result of the performed study, some commercial enteric polymers (acrylates), as well as cellulose acetate phthalate, were selected as components that have the ability to coalesce and form a continuous phase within a gelatin film.
30960322	5	30	theme	study	763:767	arg1	result					739:744	a result	737:744	a result of the performed study	737:767	As a result of the performed study, some commercial enteric polymers (acrylates), as well as cellulose acetate phthalate, were selected as components that have the ability to coalesce and form a continuous phase within a gelatin film.
30960322	5	30	theme	study	763:767	arg1	phthalate					845:853	cellulose acetate phthalate	827:853	cellulose acetate phthalate	827:853	As a result of the performed study, some commercial enteric polymers (acrylates), as well as cellulose acetate phthalate, were selected as components that have the ability to coalesce and form a continuous phase within a gelatin film.
30960322	1	31	theme	following	122:130	arg1	investigation					132:144	The following investigation	118:144	The following investigation	118:144	The following investigation comprised the formation of acid-resistant gelatin-based films, intended for future use in soft-capsule technology.
30960322	6	32	theme	rheological	1046:1056	arg1	characteristics					1058:1072	rheological characteristics	1046:1072	rheological characteristics	1046:1072	The use of a small amount (1.5%) of additional gelling polymers improved the rheological characteristics and adhesive properties of the obtained films, with ί-carrageenan and gellan gum appearing to be the most beneficial.
30960322	3	33	theme	polysaccharides	472:486	arg1	amounts					453:459	small amounts	447:459	small amounts of anionic polysaccharides that have potential to interact with gelatin, forming polyelectrolyte complexes	447:566	The selected films were additionally modified with small amounts of anionic polysaccharides that have potential to interact with gelatin, forming polyelectrolyte complexes.
30960322	3	33	theme	polysaccharides	472:486	arg1	polysaccharides					472:486	anionic polysaccharides	464:486	anionic polysaccharides that have potential to interact with gelatin, forming polyelectrolyte complexes	464:566	The selected films were additionally modified with small amounts of anionic polysaccharides that have potential to interact with gelatin, forming polyelectrolyte complexes.
30960322	1	34	theme	soft-capsule	236:247	arg1	technology					249:258	soft-capsule technology	236:258	soft-capsule technology	236:258	The following investigation comprised the formation of acid-resistant gelatin-based films, intended for future use in soft-capsule technology.
30960322	0	35	theme	Acidic	28:33	arg1	Selection					73:81	Acidic and Polyelectrolyte Polymers-Material Selection	28:81	Acidic and Polyelectrolyte Polymers-Material Selection for Soft Gastroresistant Capsules	28:115	Gelatin Films Modified with Acidic and Polyelectrolyte Polymers-Material Selection for Soft Gastroresistant Capsules.
30960322	2	36	theme	film	266:269	arg1	compositions					271:282	Such film compositions	261:282	Such film compositions	261:282	Such film compositions were obtained by including nonionized forms of acid-insoluble polymers in a gelatin-based film-forming mixture.
30960322	0	37	theme	Polymers-Material	55:71	arg1	Selection					73:81	Acidic and Polyelectrolyte Polymers-Material Selection	28:81	Acidic and Polyelectrolyte Polymers-Material Selection for Soft Gastroresistant Capsules	28:115	Gelatin Films Modified with Acidic and Polyelectrolyte Polymers-Material Selection for Soft Gastroresistant Capsules.
30960322	5	38	theme	acetate	837:843	arg1	polymers					794:801	some commercial enteric polymers	770:801	some commercial enteric polymers (acrylates)	770:813	As a result of the performed study, some commercial enteric polymers (acrylates), as well as cellulose acetate phthalate, were selected as components that have the ability to coalesce and form a continuous phase within a gelatin film.
30960322	5	38	theme	acetate	837:843	arg1	result					739:744	a result	737:744	a result of the performed study	737:767	As a result of the performed study, some commercial enteric polymers (acrylates), as well as cellulose acetate phthalate, were selected as components that have the ability to coalesce and form a continuous phase within a gelatin film.
30960322	5	38	theme	acetate	837:843	arg1	phthalate					845:853	cellulose acetate phthalate	827:853	cellulose acetate phthalate	827:853	As a result of the performed study, some commercial enteric polymers (acrylates), as well as cellulose acetate phthalate, were selected as components that have the ability to coalesce and form a continuous phase within a gelatin film.
30960322	5	38	theme	acetate	837:843	arg1	components					873:882	components	873:882	components that have the ability to coalesce and form a continuous phase within a gelatin film	873:966	As a result of the performed study, some commercial enteric polymers (acrylates), as well as cellulose acetate phthalate, were selected as components that have the ability to coalesce and form a continuous phase within a gelatin film.
30960322	2	39	theme	Such	261:264	arg1	compositions					271:282	Such film compositions	261:282	Such film compositions	261:282	Such film compositions were obtained by including nonionized forms of acid-insoluble polymers in a gelatin-based film-forming mixture.
30960322	6	40	theme	gellan	1144:1149	arg1	ί-carrageenan					1126:1138	ί-carrageenan	1126:1138	ί-carrageenan	1126:1138	The use of a small amount (1.5%) of additional gelling polymers improved the rheological characteristics and adhesive properties of the obtained films, with ί-carrageenan and gellan gum appearing to be the most beneficial.
30960322	6	40	theme	gellan	1144:1149	arg1	gum					1151:1153	gellan gum	1144:1153	gellan gum	1144:1153	The use of a small amount (1.5%) of additional gelling polymers improved the rheological characteristics and adhesive properties of the obtained films, with ί-carrageenan and gellan gum appearing to be the most beneficial.
30960322	6	40	theme	gellan	1144:1149	arg1	beneficial					1180:1189	beneficial	1180:1189	beneficial	1180:1189	The use of a small amount (1.5%) of additional gelling polymers improved the rheological characteristics and adhesive properties of the obtained films, with ί-carrageenan and gellan gum appearing to be the most beneficial.
30960322	0	41	theme	Polyelectrolyte	39:53	arg1	Selection					73:81	Acidic and Polyelectrolyte Polymers-Material Selection	28:81	Acidic and Polyelectrolyte Polymers-Material Selection for Soft Gastroresistant Capsules	28:115	Gelatin Films Modified with Acidic and Polyelectrolyte Polymers-Material Selection for Soft Gastroresistant Capsules.
30960322	1	42	from	use	229:231	arg1	technology					249:258	soft-capsule technology	236:258	soft-capsule technology	236:258	The following investigation comprised the formation of acid-resistant gelatin-based films, intended for future use in soft-capsule technology.
30960322	3	43	contain	have	493:496	arg1	polysaccharides					472:486	anionic polysaccharides	464:486	anionic polysaccharides that have potential to interact with gelatin, forming polyelectrolyte complexes	464:566	The selected films were additionally modified with small amounts of anionic polysaccharides that have potential to interact with gelatin, forming polyelectrolyte complexes.
30960322	3	43	contain	have	493:496	arg2	potential					498:506	potential	498:506	potential	498:506	The selected films were additionally modified with small amounts of anionic polysaccharides that have potential to interact with gelatin, forming polyelectrolyte complexes.
30960322	6	44	theme	gelling	1016:1022	arg1	polymers					1024:1031	additional gelling polymers	1005:1031	additional gelling polymers	1005:1031	The use of a small amount (1.5%) of additional gelling polymers improved the rheological characteristics and adhesive properties of the obtained films, with ί-carrageenan and gellan gum appearing to be the most beneficial.
30960322	3	45	theme	polyelectrolyte	542:556	arg1	complexes					558:566	polyelectrolyte complexes	542:566	polyelectrolyte complexes	542:566	The selected films were additionally modified with small amounts of anionic polysaccharides that have potential to interact with gelatin, forming polyelectrolyte complexes.
30960322	6	46	theme	polymers	1024:1031	arg1	%					999:999	1.5%	996:999	1.5%	996:999	The use of a small amount (1.5%) of additional gelling polymers improved the rheological characteristics and adhesive properties of the obtained films, with ί-carrageenan and gellan gum appearing to be the most beneficial.
30960322	6	46	theme	polymers	1024:1031	arg1	polymers					1024:1031	additional gelling polymers	1005:1031	additional gelling polymers	1005:1031	The use of a small amount (1.5%) of additional gelling polymers improved the rheological characteristics and adhesive properties of the obtained films, with ί-carrageenan and gellan gum appearing to be the most beneficial.
30960322	6	46	theme	polymers	1024:1031	arg1	amount					988:993	a small amount	980:993	a small amount (1.5%) of additional gelling polymers	980:1031	The use of a small amount (1.5%) of additional gelling polymers improved the rheological characteristics and adhesive properties of the obtained films, with ί-carrageenan and gellan gum appearing to be the most beneficial.
30960322	2	47	theme	film-forming	374:385	arg1	mixture					387:393	a gelatin-based film-forming mixture	358:393	a gelatin-based film-forming mixture	358:393	Such film compositions were obtained by including nonionized forms of acid-insoluble polymers in a gelatin-based film-forming mixture.
30960322	4	48	theme	scanning	680:687	arg1	calorimetry					689:699	differential scanning calorimetry	667:699	differential scanning calorimetry (DSC)	667:705	The obtained film compositions were subjected to, e.g., disintegration tests, adhesiveness tests, differential scanning calorimetry (DSC), and a transparency study.
30960322	4	48	theme	scanning	680:687	arg1	DSC					702:704	DSC	702:704	DSC	702:704	The obtained film compositions were subjected to, e.g., disintegration tests, adhesiveness tests, differential scanning calorimetry (DSC), and a transparency study.
30960322	6	49	theme	films	1114:1118	arg1	characteristics					1058:1072	rheological characteristics	1046:1072	rheological characteristics	1046:1072	The use of a small amount (1.5%) of additional gelling polymers improved the rheological characteristics and adhesive properties of the obtained films, with ί-carrageenan and gellan gum appearing to be the most beneficial.
30960322	6	49	theme	films	1114:1118	arg1	properties					1087:1096	adhesive properties	1078:1096	adhesive properties	1078:1096	The use of a small amount (1.5%) of additional gelling polymers improved the rheological characteristics and adhesive properties of the obtained films, with ί-carrageenan and gellan gum appearing to be the most beneficial.
30960322	6	50	theme	additional	1005:1014	arg1	polymers					1024:1031	additional gelling polymers	1005:1031	additional gelling polymers	1005:1031	The use of a small amount (1.5%) of additional gelling polymers improved the rheological characteristics and adhesive properties of the obtained films, with ί-carrageenan and gellan gum appearing to be the most beneficial.
30960322	5	51	theme	cellulose	827:835	arg1	polymers					794:801	some commercial enteric polymers	770:801	some commercial enteric polymers (acrylates)	770:813	As a result of the performed study, some commercial enteric polymers (acrylates), as well as cellulose acetate phthalate, were selected as components that have the ability to coalesce and form a continuous phase within a gelatin film.
30960322	5	51	theme	cellulose	827:835	arg1	result					739:744	a result	737:744	a result of the performed study	737:767	As a result of the performed study, some commercial enteric polymers (acrylates), as well as cellulose acetate phthalate, were selected as components that have the ability to coalesce and form a continuous phase within a gelatin film.
30960322	5	51	theme	cellulose	827:835	arg1	phthalate					845:853	cellulose acetate phthalate	827:853	cellulose acetate phthalate	827:853	As a result of the performed study, some commercial enteric polymers (acrylates), as well as cellulose acetate phthalate, were selected as components that have the ability to coalesce and form a continuous phase within a gelatin film.
30960322	5	51	theme	cellulose	827:835	arg1	components					873:882	components	873:882	components that have the ability to coalesce and form a continuous phase within a gelatin film	873:966	As a result of the performed study, some commercial enteric polymers (acrylates), as well as cellulose acetate phthalate, were selected as components that have the ability to coalesce and form a continuous phase within a gelatin film.
30960322	4	52	theme	film	582:585	arg1	compositions					587:598	The obtained film compositions	569:598	The obtained film compositions	569:598	The obtained film compositions were subjected to, e.g., disintegration tests, adhesiveness tests, differential scanning calorimetry (DSC), and a transparency study.
30960322	1	53	theme	acid-resistant	173:186	arg1	films					202:206	acid-resistant gelatin-based films	173:206	acid-resistant gelatin-based films	173:206	The following investigation comprised the formation of acid-resistant gelatin-based films, intended for future use in soft-capsule technology.
30960322	5	54	theme	enteric	786:792	arg1	polymers					794:801	some commercial enteric polymers	770:801	some commercial enteric polymers (acrylates)	770:813	As a result of the performed study, some commercial enteric polymers (acrylates), as well as cellulose acetate phthalate, were selected as components that have the ability to coalesce and form a continuous phase within a gelatin film.
30960322	5	54	theme	enteric	786:792	arg1	phthalate					845:853	cellulose acetate phthalate	827:853	cellulose acetate phthalate	827:853	As a result of the performed study, some commercial enteric polymers (acrylates), as well as cellulose acetate phthalate, were selected as components that have the ability to coalesce and form a continuous phase within a gelatin film.
30960322	5	54	theme	enteric	786:792	arg1	result					739:744	a result	737:744	a result of the performed study	737:767	As a result of the performed study, some commercial enteric polymers (acrylates), as well as cellulose acetate phthalate, were selected as components that have the ability to coalesce and form a continuous phase within a gelatin film.
30960322	5	54	theme	enteric	786:792	arg1	acrylates					804:812	acrylates	804:812	acrylates	804:812	As a result of the performed study, some commercial enteric polymers (acrylates), as well as cellulose acetate phthalate, were selected as components that have the ability to coalesce and form a continuous phase within a gelatin film.
30960322	5	54	theme	enteric	786:792	arg1	components					873:882	components	873:882	components that have the ability to coalesce and form a continuous phase within a gelatin film	873:966	As a result of the performed study, some commercial enteric polymers (acrylates), as well as cellulose acetate phthalate, were selected as components that have the ability to coalesce and form a continuous phase within a gelatin film.
30320890	2	0	theme	-induced	307:314	arg1	disruption					316:325	ethanol (EtOH)-induced disruption	293:325	ethanol (EtOH)-induced disruption of gut-liver homeostasis	293:350	However, it is unclear how changes in the intestinal microbiota (IM) relate to ethanol (EtOH)-induced disruption of gut-liver homeostasis.
30320890	5	1	theme	Group	754:758	arg1	groups					746:751	Control groups	738:751	Control groups (Group Ct)	738:762	Control groups (Group Ct) were composed of matched-age EtOH-naïve sP rats.
30320890	5	1	theme	Group	754:758	arg1	Ct					760:761	Group Ct	754:761	Group Ct	754:761	Control groups (Group Ct) were composed of matched-age EtOH-naïve sP rats.
30320890	7	2	from	Rats	1076:1079	arg1	Group					1084:1088	Group	1084:1088	Group	1084:1088	RESULTS Rats in Group Et developed hepatic steatosis and elevated serum transaminases and endotoxin/lipopolysaccharide (LPS) levels but no other liver pathological changes (i.e., necrosis/inflammation) or systemic inflammation.
30320890	4	3	theme	%	606:606	arg1	2-bottle					588:595	2-bottle	588:595	2-bottle	588:595	METHODS Independent groups of male adult sP rats were exposed to the standard, home-cage 2-bottle "EtOH (10% v/v) versus water" choice regimen with unlimited access for 24 h/d (Group Et) for 3 (T1), 6 (T2), and 12 (T3) consecutive months.
30320890	4	3	theme	%	606:606	arg1	v/v					608:610	10% v/v	604:610	10% v/v	604:610	METHODS Independent groups of male adult sP rats were exposed to the standard, home-cage 2-bottle "EtOH (10% v/v) versus water" choice regimen with unlimited access for 24 h/d (Group Et) for 3 (T1), 6 (T2), and 12 (T3) consecutive months.
30320890	8	4	from	rats	1390:1393	arg1	Group					1398:1402	Group	1398:1402	Group	1398:1402	While we did not find any apparent alteration of the intestinal colonic mucosa, we found that rats in Group Et exhibited significant changes in IM composition compared to the rats in Group Ct.
30320890	7	5	theme	pathological	1219:1230	arg1	changes					1232:1238	no other liver pathological changes	1204:1238	no other liver pathological changes	1204:1238	RESULTS Rats in Group Et developed hepatic steatosis and elevated serum transaminases and endotoxin/lipopolysaccharide (LPS) levels but no other liver pathological changes (i.e., necrosis/inflammation) or systemic inflammation.
30320890	8	6	from	changes	1429:1435	arg1	composition					1443:1453	IM composition	1440:1453	IM composition	1440:1453	While we did not find any apparent alteration of the intestinal colonic mucosa, we found that rats in Group Et exhibited significant changes in IM composition compared to the rats in Group Ct.
30320890	3	7	theme	excessive	471:479	arg1	consumption					486:496	excessive EtOH consumption	471:496	excessive EtOH consumption	471:496	We investigated this issue using selectively bred Sardinian alcohol-preferring (sP) rats, a validated animal model of excessive EtOH consumption.
30320890	13	8	theme	diseases	2065:2072	arg1	treatment					2039:2047	treatment	2039:2047	treatment	2039:2047	This work sets the stage for improving our knowledge of the prevention and treatment of EtOH-related diseases.
30320890	13	8	theme	diseases	2065:2072	arg1	prevention					2024:2033	prevention	2024:2033	prevention	2024:2033	This work sets the stage for improving our knowledge of the prevention and treatment of EtOH-related diseases.
30320890	7	9	theme	other	1207:1211	arg1	changes					1232:1238	no other liver pathological changes	1204:1238	no other liver pathological changes	1204:1238	RESULTS Rats in Group Et developed hepatic steatosis and elevated serum transaminases and endotoxin/lipopolysaccharide (LPS) levels but no other liver pathological changes (i.e., necrosis/inflammation) or systemic inflammation.
30320890	2	10	theme	homeostasis	340:350	arg1	disruption					316:325	ethanol (EtOH)-induced disruption	293:325	ethanol (EtOH)-induced disruption of gut-liver homeostasis	293:350	However, it is unclear how changes in the intestinal microbiota (IM) relate to ethanol (EtOH)-induced disruption of gut-liver homeostasis.
30320890	8	11	theme	IM	1440:1441	arg1	composition					1443:1453	IM composition	1440:1453	IM composition	1440:1453	While we did not find any apparent alteration of the intestinal colonic mucosa, we found that rats in Group Et exhibited significant changes in IM composition compared to the rats in Group Ct.
30320890	6	12	theme	ribosomal	1037:1045	arg1	sequencing					1056:1065	ribosomal RNA gene sequencing	1037:1065	ribosomal RNA gene sequencing	1037:1065	We obtained samples from each rat at the end of each experimental time, and we used blood and colon tissues for intestinal barrier integrity and/or liver pathology assessments and used stool samples for IM analysis with 16S ribosomal RNA gene sequencing.
30320890	7	13	dep	necrosis/inflammation	1247:1267	arg1	i.e.					1241:1244	i.e.	1241:1244	i.e.	1241:1244	RESULTS Rats in Group Et developed hepatic steatosis and elevated serum transaminases and endotoxin/lipopolysaccharide (LPS) levels but no other liver pathological changes (i.e., necrosis/inflammation) or systemic inflammation.
30320890	12	14	theme	chronic	1847:1853	arg1	consumption					1871:1881	chronic, voluntary EtOH consumption	1847:1881	chronic, voluntary EtOH consumption	1847:1881	CONCLUSIONS We showed that chronic, voluntary EtOH consumption induced liver injury and endotoxemia together with dysbiotic changes in sP rats.
30320890	10	15	theme	abundant	1629:1636	arg1	Streptococcus					1591:1603	Streptococcus	1591:1603	Streptococcus	1591:1603	In particular, Ruminococcus, Coprococcus, and Streptococcus were the differentially abundant microbial genera at T3.
30320890	10	15	theme	abundant	1629:1636	arg1	Ruminococcus					1560:1571	Ruminococcus	1560:1571	Ruminococcus	1560:1571	In particular, Ruminococcus, Coprococcus, and Streptococcus were the differentially abundant microbial genera at T3.
30320890	10	15	theme	abundant	1629:1636	arg1	genera					1648:1653	the differentially abundant microbial genera	1610:1653	the differentially abundant microbial genera	1610:1653	In particular, Ruminococcus, Coprococcus, and Streptococcus were the differentially abundant microbial genera at T3.
30320890	10	15	theme	abundant	1629:1636	arg1	Coprococcus					1574:1584	Coprococcus	1574:1584	Coprococcus	1574:1584	In particular, Ruminococcus, Coprococcus, and Streptococcus were the differentially abundant microbial genera at T3.
30320890	1	16	theme	strong	135:140	arg1	evidence					142:149	strong evidence that alcoholism leads to dysbiosis in both humans and animals	135:211	strong evidence that alcoholism leads to dysbiosis in both humans and animals	135:211	BACKGROUND There is strong evidence that alcoholism leads to dysbiosis in both humans and animals.
30320890	6	17	theme	IM	1016:1017	arg1	analysis					1019:1026	IM analysis	1016:1026	IM analysis	1016:1026	We obtained samples from each rat at the end of each experimental time, and we used blood and colon tissues for intestinal barrier integrity and/or liver pathology assessments and used stool samples for IM analysis with 16S ribosomal RNA gene sequencing.
30320890	4	18	theme	water	620:624	arg1	regimen					634:640	water" choice regimen	620:640	water" choice regimen with unlimited access for 24 h/d (Group Et)	620:684	METHODS Independent groups of male adult sP rats were exposed to the standard, home-cage 2-bottle "EtOH (10% v/v) versus water" choice regimen with unlimited access for 24 h/d (Group Et) for 3 (T1), 6 (T2), and 12 (T3) consecutive months.
30320890	5	19	theme	Control	738:744	arg1	groups					746:751	Control groups	738:751	Control groups (Group Ct)	738:762	Control groups (Group Ct) were composed of matched-age EtOH-naïve sP rats.
30320890	5	19	theme	Control	738:744	arg1	Ct					760:761	Group Ct	754:761	Group Ct	754:761	Control groups (Group Ct) were composed of matched-age EtOH-naïve sP rats.
30320890	4	20	theme	choice	627:632	arg1	regimen					634:640	water" choice regimen	620:640	water" choice regimen with unlimited access for 24 h/d (Group Et)	620:684	METHODS Independent groups of male adult sP rats were exposed to the standard, home-cage 2-bottle "EtOH (10% v/v) versus water" choice regimen with unlimited access for 24 h/d (Group Et) for 3 (T1), 6 (T2), and 12 (T3) consecutive months.
30320890	0	21	theme	Microbiota	64:73	arg1	Composition					75:85	Intestinal Microbiota Composition	53:85	Intestinal Microbiota Composition	53:85	Liver Injury, Endotoxemia, and Their Relationship to Intestinal Microbiota Composition in Alcohol-Preferring Rats.
30320890	4	22	dep	METHODS	499:505	arg1	exposed					553:559	exposed	553:559	were exposed to the standard, home-cage 2-bottle "EtOH (10% v/v) versus water" choice regimen with unlimited access for 24 h/d (Group Et) for 3 (T1), 6 (T2), and 12 (T3) consecutive months	548:735	METHODS Independent groups of male adult sP rats were exposed to the standard, home-cage 2-bottle "EtOH (10% v/v) versus water" choice regimen with unlimited access for 24 h/d (Group Et) for 3 (T1), 6 (T2), and 12 (T3) consecutive months.
30320890	11	23	theme	LPS	1772:1774	arg1	transport					1747:1755	transport	1747:1755	transport	1747:1755	The KEGG Ortholog profile revealed that IM functional modules, such as biosynthesis, transport, and export of LPS, were also enriched in Group Et rats at T3.
30320890	11	23	theme	LPS	1772:1774	arg1	export					1762:1767	export	1762:1767	export	1762:1767	The KEGG Ortholog profile revealed that IM functional modules, such as biosynthesis, transport, and export of LPS, were also enriched in Group Et rats at T3.
30320890	11	23	theme	LPS	1772:1774	arg1	biosynthesis					1733:1744	biosynthesis	1733:1744	biosynthesis	1733:1744	The KEGG Ortholog profile revealed that IM functional modules, such as biosynthesis, transport, and export of LPS, were also enriched in Group Et rats at T3.
30320890	13	24	dep	prevention	2024:2033	arg1	the					2020:2022	the	2020:2022	the	2020:2022	This work sets the stage for improving our knowledge of the prevention and treatment of EtOH-related diseases.
30320890	7	25	theme	elevated	1125:1132	arg1	levels					1193:1198	elevated serum transaminases and endotoxin/lipopolysaccharide (LPS) levels	1125:1198	elevated serum transaminases and endotoxin/lipopolysaccharide (LPS) levels	1125:1198	RESULTS Rats in Group Et developed hepatic steatosis and elevated serum transaminases and endotoxin/lipopolysaccharide (LPS) levels but no other liver pathological changes (i.e., necrosis/inflammation) or systemic inflammation.
30320890	1	26	dep	BACKGROUND	115:124	arg1	is					132:133	is	132:133	is strong evidence that alcoholism leads to dysbiosis in both humans and animals	132:211	BACKGROUND There is strong evidence that alcoholism leads to dysbiosis in both humans and animals.
30320890	3	27	theme	alcohol-preferring	413:430	arg1	model					462:466	a validated animal model	443:466	a validated animal model of excessive EtOH consumption	443:496	We investigated this issue using selectively bred Sardinian alcohol-preferring (sP) rats, a validated animal model of excessive EtOH consumption.
30320890	3	27	theme	alcohol-preferring	413:430	arg1	rats					437:440	selectively bred Sardinian alcohol-preferring (sP) rats	386:440	selectively bred Sardinian alcohol-preferring (sP) rats	386:440	We investigated this issue using selectively bred Sardinian alcohol-preferring (sP) rats, a validated animal model of excessive EtOH consumption.
30320890	7	28	theme	endotoxin/lipopolysaccharide	1158:1185	arg1	levels					1193:1198	elevated serum transaminases and endotoxin/lipopolysaccharide (LPS) levels	1125:1198	elevated serum transaminases and endotoxin/lipopolysaccharide (LPS) levels	1125:1198	RESULTS Rats in Group Et developed hepatic steatosis and elevated serum transaminases and endotoxin/lipopolysaccharide (LPS) levels but no other liver pathological changes (i.e., necrosis/inflammation) or systemic inflammation.
30320890	8	29	theme	colonic	1360:1366	arg1	mucosa					1368:1373	the intestinal colonic mucosa	1345:1373	the intestinal colonic mucosa	1345:1373	While we did not find any apparent alteration of the intestinal colonic mucosa, we found that rats in Group Et exhibited significant changes in IM composition compared to the rats in Group Ct.
30320890	3	30	theme	bred	398:401	arg1	model					462:466	a validated animal model	443:466	a validated animal model of excessive EtOH consumption	443:496	We investigated this issue using selectively bred Sardinian alcohol-preferring (sP) rats, a validated animal model of excessive EtOH consumption.
30320890	3	30	theme	bred	398:401	arg1	rats					437:440	selectively bred Sardinian alcohol-preferring (sP) rats	386:440	selectively bred Sardinian alcohol-preferring (sP) rats	386:440	We investigated this issue using selectively bred Sardinian alcohol-preferring (sP) rats, a validated animal model of excessive EtOH consumption.
30320890	0	31	theme	Liver	0:4	arg1	Injury					6:11	Liver Injury	0:11	Liver Injury	0:11	Liver Injury, Endotoxemia, and Their Relationship to Intestinal Microbiota Composition in Alcohol-Preferring Rats.
30320890	7	32	theme	transaminases	1140:1152	arg1	levels					1193:1198	elevated serum transaminases and endotoxin/lipopolysaccharide (LPS) levels	1125:1198	elevated serum transaminases and endotoxin/lipopolysaccharide (LPS) levels	1125:1198	RESULTS Rats in Group Et developed hepatic steatosis and elevated serum transaminases and endotoxin/lipopolysaccharide (LPS) levels but no other liver pathological changes (i.e., necrosis/inflammation) or systemic inflammation.
30320890	5	33	theme	EtOH-naïve	793:802	arg1	rats					807:810	matched-age EtOH-naïve sP rats	781:810	matched-age EtOH-naïve sP rats	781:810	Control groups (Group Ct) were composed of matched-age EtOH-naïve sP rats.
30320890	6	34	theme	experimental	866:877	arg1	time					879:882	each experimental time	861:882	each experimental time	861:882	We obtained samples from each rat at the end of each experimental time, and we used blood and colon tissues for intestinal barrier integrity and/or liver pathology assessments and used stool samples for IM analysis with 16S ribosomal RNA gene sequencing.
30320890	4	35	theme	adult	534:538	arg1	rats					543:546	male adult sP rats	529:546	male adult sP rats	529:546	METHODS Independent groups of male adult sP rats were exposed to the standard, home-cage 2-bottle "EtOH (10% v/v) versus water" choice regimen with unlimited access for 24 h/d (Group Et) for 3 (T1), 6 (T2), and 12 (T3) consecutive months.
30320890	12	36	theme	liver	1891:1895	arg1	injury					1897:1902	liver injury	1891:1902	liver injury	1891:1902	CONCLUSIONS We showed that chronic, voluntary EtOH consumption induced liver injury and endotoxemia together with dysbiotic changes in sP rats.
30320890	3	37	theme	animal	455:460	arg1	model					462:466	a validated animal model	443:466	a validated animal model of excessive EtOH consumption	443:496	We investigated this issue using selectively bred Sardinian alcohol-preferring (sP) rats, a validated animal model of excessive EtOH consumption.
30320890	3	37	theme	animal	455:460	arg1	rats					437:440	selectively bred Sardinian alcohol-preferring (sP) rats	386:440	selectively bred Sardinian alcohol-preferring (sP) rats	386:440	We investigated this issue using selectively bred Sardinian alcohol-preferring (sP) rats, a validated animal model of excessive EtOH consumption.
30320890	0	38	from	Endotoxemia	14:24	arg1	Rats					109:112	Alcohol-Preferring Rats	90:112	Alcohol-Preferring Rats	90:112	Liver Injury, Endotoxemia, and Their Relationship to Intestinal Microbiota Composition in Alcohol-Preferring Rats.
30320890	4	39	dep	2-bottle	588:595	arg1	EtOH					598:601	EtOH	598:601	home-cage 2-bottle "EtOH (10% v/v)	578:611	METHODS Independent groups of male adult sP rats were exposed to the standard, home-cage 2-bottle "EtOH (10% v/v) versus water" choice regimen with unlimited access for 24 h/d (Group Et) for 3 (T1), 6 (T2), and 12 (T3) consecutive months.
30320890	6	40	theme	barrier	936:942	arg1	integrity					944:952	intestinal barrier integrity	925:952	intestinal barrier integrity	925:952	We obtained samples from each rat at the end of each experimental time, and we used blood and colon tissues for intestinal barrier integrity and/or liver pathology assessments and used stool samples for IM analysis with 16S ribosomal RNA gene sequencing.
30320890	4	41	theme	Independent	507:517	arg1	rats					543:546	male adult sP rats	529:546	male adult sP rats	529:546	METHODS Independent groups of male adult sP rats were exposed to the standard, home-cage 2-bottle "EtOH (10% v/v) versus water" choice regimen with unlimited access for 24 h/d (Group Et) for 3 (T1), 6 (T2), and 12 (T3) consecutive months.
30320890	4	41	theme	Independent	507:517	arg1	groups					519:524	Independent groups	507:524	Independent groups of male adult sP rats	507:546	METHODS Independent groups of male adult sP rats were exposed to the standard, home-cage 2-bottle "EtOH (10% v/v) versus water" choice regimen with unlimited access for 24 h/d (Group Et) for 3 (T1), 6 (T2), and 12 (T3) consecutive months.
30320890	6	42	dep	blood	897:901	arg1	tissues					913:919	tissues	913:919	tissues	913:919	We obtained samples from each rat at the end of each experimental time, and we used blood and colon tissues for intestinal barrier integrity and/or liver pathology assessments and used stool samples for IM analysis with 16S ribosomal RNA gene sequencing.
30320890	12	43	theme	dysbiotic	1934:1942	arg1	changes					1944:1950	dysbiotic changes	1934:1950	dysbiotic changes in sP rats	1934:1961	CONCLUSIONS We showed that chronic, voluntary EtOH consumption induced liver injury and endotoxemia together with dysbiotic changes in sP rats.
30320890	3	44	theme	EtOH	481:484	arg1	consumption					486:496	excessive EtOH consumption	471:496	excessive EtOH consumption	471:496	We investigated this issue using selectively bred Sardinian alcohol-preferring (sP) rats, a validated animal model of excessive EtOH consumption.
30320890	12	45	from	changes	1944:1950	arg1	rats					1958:1961	sP rats	1955:1961	sP rats	1955:1961	CONCLUSIONS We showed that chronic, voluntary EtOH consumption induced liver injury and endotoxemia together with dysbiotic changes in sP rats.
30320890	10	46	from	genera	1648:1653	arg1	particular					1548:1557	particular	1548:1557	particular	1548:1557	In particular, Ruminococcus, Coprococcus, and Streptococcus were the differentially abundant microbial genera at T3.
30320890	6	47	theme	colon	907:911	arg1	assessments					977:987	blood and colon tissues for intestinal barrier integrity and/or liver pathology assessments	897:987	blood and colon tissues for intestinal barrier integrity and/or liver pathology assessments	897:987	We obtained samples from each rat at the end of each experimental time, and we used blood and colon tissues for intestinal barrier integrity and/or liver pathology assessments and used stool samples for IM analysis with 16S ribosomal RNA gene sequencing.
30320890	10	48	from	T3	1658:1659	arg1	Streptococcus					1591:1603	Streptococcus	1591:1603	Streptococcus	1591:1603	In particular, Ruminococcus, Coprococcus, and Streptococcus were the differentially abundant microbial genera at T3.
30320890	10	48	from	T3	1658:1659	arg1	Ruminococcus					1560:1571	Ruminococcus	1560:1571	Ruminococcus	1560:1571	In particular, Ruminococcus, Coprococcus, and Streptococcus were the differentially abundant microbial genera at T3.
30320890	10	48	from	T3	1658:1659	arg1	genera					1648:1653	the differentially abundant microbial genera	1610:1653	the differentially abundant microbial genera	1610:1653	In particular, Ruminococcus, Coprococcus, and Streptococcus were the differentially abundant microbial genera at T3.
30320890	10	48	from	T3	1658:1659	arg1	Coprococcus					1574:1584	Coprococcus	1574:1584	Coprococcus	1574:1584	In particular, Ruminococcus, Coprococcus, and Streptococcus were the differentially abundant microbial genera at T3.
30320890	6	49	theme	blood	897:901	arg1	assessments					977:987	blood and colon tissues for intestinal barrier integrity and/or liver pathology assessments	897:987	blood and colon tissues for intestinal barrier integrity and/or liver pathology assessments	897:987	We obtained samples from each rat at the end of each experimental time, and we used blood and colon tissues for intestinal barrier integrity and/or liver pathology assessments and used stool samples for IM analysis with 16S ribosomal RNA gene sequencing.
30320890	4	50	theme	rats	543:546	arg1	rats					543:546	male adult sP rats	529:546	male adult sP rats	529:546	METHODS Independent groups of male adult sP rats were exposed to the standard, home-cage 2-bottle "EtOH (10% v/v) versus water" choice regimen with unlimited access for 24 h/d (Group Et) for 3 (T1), 6 (T2), and 12 (T3) consecutive months.
30320890	4	50	theme	rats	543:546	arg1	groups					519:524	Independent groups	507:524	Independent groups of male adult sP rats	507:546	METHODS Independent groups of male adult sP rats were exposed to the standard, home-cage 2-bottle "EtOH (10% v/v) versus water" choice regimen with unlimited access for 24 h/d (Group Et) for 3 (T1), 6 (T2), and 12 (T3) consecutive months.
30320890	4	51	with	regimen	634:640	arg1	access					657:662	unlimited access	647:662	unlimited access	647:662	METHODS Independent groups of male adult sP rats were exposed to the standard, home-cage 2-bottle "EtOH (10% v/v) versus water" choice regimen with unlimited access for 24 h/d (Group Et) for 3 (T1), 6 (T2), and 12 (T3) consecutive months.
30320890	4	52	theme	consecutive	718:728	arg1	months					730:735	3 (T1), 6 (T2), and 12 (T3) consecutive months	690:735	3 (T1), 6 (T2), and 12 (T3) consecutive months	690:735	METHODS Independent groups of male adult sP rats were exposed to the standard, home-cage 2-bottle "EtOH (10% v/v) versus water" choice regimen with unlimited access for 24 h/d (Group Et) for 3 (T1), 6 (T2), and 12 (T3) consecutive months.
30320890	6	53	theme	stool	998:1002	arg1	samples					1004:1010	stool samples	998:1010	stool samples for IM analysis	998:1026	We obtained samples from each rat at the end of each experimental time, and we used blood and colon tissues for intestinal barrier integrity and/or liver pathology assessments and used stool samples for IM analysis with 16S ribosomal RNA gene sequencing.
30320890	2	54	theme	intestinal	256:265	arg1	microbiota					267:276	the intestinal microbiota	252:276	the intestinal microbiota (IM)	252:281	However, it is unclear how changes in the intestinal microbiota (IM) relate to ethanol (EtOH)-induced disruption of gut-liver homeostasis.
30320890	2	54	theme	intestinal	256:265	arg1	IM					279:280	IM	279:280	IM	279:280	However, it is unclear how changes in the intestinal microbiota (IM) relate to ethanol (EtOH)-induced disruption of gut-liver homeostasis.
30320890	7	55	theme	systemic	1273:1280	arg1	inflammation					1282:1293	systemic inflammation	1273:1293	systemic inflammation	1273:1293	RESULTS Rats in Group Et developed hepatic steatosis and elevated serum transaminases and endotoxin/lipopolysaccharide (LPS) levels but no other liver pathological changes (i.e., necrosis/inflammation) or systemic inflammation.
30320890	11	56	theme	Et	1805:1806	arg1	rats					1808:1811	Group Et rats	1799:1811	Group Et rats	1799:1811	The KEGG Ortholog profile revealed that IM functional modules, such as biosynthesis, transport, and export of LPS, were also enriched in Group Et rats at T3.
30320890	4	57	theme	12	710:711	arg1	months					730:735	3 (T1), 6 (T2), and 12 (T3) consecutive months	690:735	3 (T1), 6 (T2), and 12 (T3) consecutive months	690:735	METHODS Independent groups of male adult sP rats were exposed to the standard, home-cage 2-bottle "EtOH (10% v/v) versus water" choice regimen with unlimited access for 24 h/d (Group Et) for 3 (T1), 6 (T2), and 12 (T3) consecutive months.
30320890	6	58	theme	pathology	967:975	arg1	assessments					977:987	blood and colon tissues for intestinal barrier integrity and/or liver pathology assessments	897:987	blood and colon tissues for intestinal barrier integrity and/or liver pathology assessments	897:987	We obtained samples from each rat at the end of each experimental time, and we used blood and colon tissues for intestinal barrier integrity and/or liver pathology assessments and used stool samples for IM analysis with 16S ribosomal RNA gene sequencing.
30320890	6	59	used	used	993:996	arg2	we					889:890	we	889:890	we	889:890	We obtained samples from each rat at the end of each experimental time, and we used blood and colon tissues for intestinal barrier integrity and/or liver pathology assessments and used stool samples for IM analysis with 16S ribosomal RNA gene sequencing.
30320890	13	60	theme	EtOH-related	2052:2063	arg1	diseases					2065:2072	EtOH-related diseases	2052:2072	EtOH-related diseases	2052:2072	This work sets the stage for improving our knowledge of the prevention and treatment of EtOH-related diseases.
30320890	7	61	theme	liver	1213:1217	arg1	changes					1232:1238	no other liver pathological changes	1204:1238	no other liver pathological changes	1204:1238	RESULTS Rats in Group Et developed hepatic steatosis and elevated serum transaminases and endotoxin/lipopolysaccharide (LPS) levels but no other liver pathological changes (i.e., necrosis/inflammation) or systemic inflammation.
30320890	12	62	theme	EtOH	1866:1869	arg1	consumption					1871:1881	chronic, voluntary EtOH consumption	1847:1881	chronic, voluntary EtOH consumption	1847:1881	CONCLUSIONS We showed that chronic, voluntary EtOH consumption induced liver injury and endotoxemia together with dysbiotic changes in sP rats.
30320890	4	63	dep	Group	676:680	arg1	Et					682:683	Group Et	676:683	Group Et	676:683	METHODS Independent groups of male adult sP rats were exposed to the standard, home-cage 2-bottle "EtOH (10% v/v) versus water" choice regimen with unlimited access for 24 h/d (Group Et) for 3 (T1), 6 (T2), and 12 (T3) consecutive months.
30320890	10	64	theme	microbial	1638:1646	arg1	Streptococcus					1591:1603	Streptococcus	1591:1603	Streptococcus	1591:1603	In particular, Ruminococcus, Coprococcus, and Streptococcus were the differentially abundant microbial genera at T3.
30320890	10	64	theme	microbial	1638:1646	arg1	Ruminococcus					1560:1571	Ruminococcus	1560:1571	Ruminococcus	1560:1571	In particular, Ruminococcus, Coprococcus, and Streptococcus were the differentially abundant microbial genera at T3.
30320890	10	64	theme	microbial	1638:1646	arg1	genera					1648:1653	the differentially abundant microbial genera	1610:1653	the differentially abundant microbial genera	1610:1653	In particular, Ruminococcus, Coprococcus, and Streptococcus were the differentially abundant microbial genera at T3.
30320890	10	64	theme	microbial	1638:1646	arg1	Coprococcus					1574:1584	Coprococcus	1574:1584	Coprococcus	1574:1584	In particular, Ruminococcus, Coprococcus, and Streptococcus were the differentially abundant microbial genera at T3.
30320890	13	65	theme	treatment	2039:2047	arg1	knowledge					2007:2015	our knowledge	2003:2015	our knowledge of the prevention and treatment of EtOH-related diseases	2003:2072	This work sets the stage for improving our knowledge of the prevention and treatment of EtOH-related diseases.
30320890	6	66	theme	liver	961:965	arg1	pathology					967:975	liver pathology	961:975	liver pathology	961:975	We obtained samples from each rat at the end of each experimental time, and we used blood and colon tissues for intestinal barrier integrity and/or liver pathology assessments and used stool samples for IM analysis with 16S ribosomal RNA gene sequencing.
30320890	4	67	theme	3	690:690	arg1	months					730:735	3 (T1), 6 (T2), and 12 (T3) consecutive months	690:735	3 (T1), 6 (T2), and 12 (T3) consecutive months	690:735	METHODS Independent groups of male adult sP rats were exposed to the standard, home-cage 2-bottle "EtOH (10% v/v) versus water" choice regimen with unlimited access for 24 h/d (Group Et) for 3 (T1), 6 (T2), and 12 (T3) consecutive months.
30320890	12	68	dep	CONCLUSIONS	1820:1830	arg1	showed					1835:1840	showed	1835:1840	showed that chronic, voluntary EtOH consumption induced liver injury and endotoxemia together with dysbiotic changes in sP rats	1835:1961	CONCLUSIONS We showed that chronic, voluntary EtOH consumption induced liver injury and endotoxemia together with dysbiotic changes in sP rats.
30320890	4	69	theme	unlimited	647:655	arg1	access					657:662	unlimited access	647:662	unlimited access	647:662	METHODS Independent groups of male adult sP rats were exposed to the standard, home-cage 2-bottle "EtOH (10% v/v) versus water" choice regimen with unlimited access for 24 h/d (Group Et) for 3 (T1), 6 (T2), and 12 (T3) consecutive months.
30320890	0	70	theme	Intestinal	53:62	arg1	Composition					75:85	Intestinal Microbiota Composition	53:85	Intestinal Microbiota Composition	53:85	Liver Injury, Endotoxemia, and Their Relationship to Intestinal Microbiota Composition in Alcohol-Preferring Rats.
30320890	8	71	theme	apparent	1322:1329	arg1	alteration					1331:1340	any apparent alteration	1318:1340	any apparent alteration of the intestinal colonic mucosa	1318:1373	While we did not find any apparent alteration of the intestinal colonic mucosa, we found that rats in Group Et exhibited significant changes in IM composition compared to the rats in Group Ct.
30320890	11	72	theme	KEGG	1666:1669	arg1	profile					1680:1686	The KEGG Ortholog profile	1662:1686	The KEGG Ortholog profile	1662:1686	The KEGG Ortholog profile revealed that IM functional modules, such as biosynthesis, transport, and export of LPS, were also enriched in Group Et rats at T3.
30320890	1	73	from	dysbiosis	176:184	arg1	animals					205:211	animals	205:211	animals	205:211	BACKGROUND There is strong evidence that alcoholism leads to dysbiosis in both humans and animals.
30320890	1	73	from	dysbiosis	176:184	arg1	humans					194:199	humans	194:199	humans	194:199	BACKGROUND There is strong evidence that alcoholism leads to dysbiosis in both humans and animals.
30320890	0	74	from	Relationship	37:48	arg1	Rats					109:112	Alcohol-Preferring Rats	90:112	Alcohol-Preferring Rats	90:112	Liver Injury, Endotoxemia, and Their Relationship to Intestinal Microbiota Composition in Alcohol-Preferring Rats.
30320890	0	75	from	Injury	6:11	arg1	Rats					109:112	Alcohol-Preferring Rats	90:112	Alcohol-Preferring Rats	90:112	Liver Injury, Endotoxemia, and Their Relationship to Intestinal Microbiota Composition in Alcohol-Preferring Rats.
30320890	6	76	from	rat	843:845	arg1	samples					825:831	samples	825:831	samples from each rat	825:845	We obtained samples from each rat at the end of each experimental time, and we used blood and colon tissues for intestinal barrier integrity and/or liver pathology assessments and used stool samples for IM analysis with 16S ribosomal RNA gene sequencing.
30320890	4	77	theme	"	625:625	arg1	regimen					634:640	water" choice regimen	620:640	water" choice regimen with unlimited access for 24 h/d (Group Et)	620:684	METHODS Independent groups of male adult sP rats were exposed to the standard, home-cage 2-bottle "EtOH (10% v/v) versus water" choice regimen with unlimited access for 24 h/d (Group Et) for 3 (T1), 6 (T2), and 12 (T3) consecutive months.
30320890	0	78	theme	Alcohol-Preferring	90:107	arg1	Rats					109:112	Alcohol-Preferring Rats	90:112	Alcohol-Preferring Rats	90:112	Liver Injury, Endotoxemia, and Their Relationship to Intestinal Microbiota Composition in Alcohol-Preferring Rats.
30320890	5	79	theme	sP	804:805	arg1	rats					807:810	matched-age EtOH-naïve sP rats	781:810	matched-age EtOH-naïve sP rats	781:810	Control groups (Group Ct) were composed of matched-age EtOH-naïve sP rats.
30320890	11	80	theme	Group	1799:1803	arg1	rats					1808:1811	Group Et rats	1799:1811	Group Et rats	1799:1811	The KEGG Ortholog profile revealed that IM functional modules, such as biosynthesis, transport, and export of LPS, were also enriched in Group Et rats at T3.
30320890	8	81	theme	intestinal	1349:1358	arg1	mucosa					1368:1373	the intestinal colonic mucosa	1345:1373	the intestinal colonic mucosa	1345:1373	While we did not find any apparent alteration of the intestinal colonic mucosa, we found that rats in Group Et exhibited significant changes in IM composition compared to the rats in Group Ct.
30320890	8	82	theme	mucosa	1368:1373	arg1	alteration					1331:1340	any apparent alteration	1318:1340	any apparent alteration of the intestinal colonic mucosa	1318:1373	While we did not find any apparent alteration of the intestinal colonic mucosa, we found that rats in Group Et exhibited significant changes in IM composition compared to the rats in Group Ct.
30320890	11	83	theme	functional	1705:1714	arg1	transport					1747:1755	transport	1747:1755	transport	1747:1755	The KEGG Ortholog profile revealed that IM functional modules, such as biosynthesis, transport, and export of LPS, were also enriched in Group Et rats at T3.
30320890	11	83	theme	functional	1705:1714	arg1	export					1762:1767	export	1762:1767	export	1762:1767	The KEGG Ortholog profile revealed that IM functional modules, such as biosynthesis, transport, and export of LPS, were also enriched in Group Et rats at T3.
30320890	11	83	theme	functional	1705:1714	arg1	biosynthesis					1733:1744	biosynthesis	1733:1744	biosynthesis	1733:1744	The KEGG Ortholog profile revealed that IM functional modules, such as biosynthesis, transport, and export of LPS, were also enriched in Group Et rats at T3.
30320890	11	83	theme	functional	1705:1714	arg1	modules					1716:1722	IM functional modules	1702:1722	IM functional modules	1702:1722	The KEGG Ortholog profile revealed that IM functional modules, such as biosynthesis, transport, and export of LPS, were also enriched in Group Et rats at T3.
30320890	12	84	dep	chronic	1847:1853	arg1	voluntary					1856:1864	voluntary	1856:1864	voluntary	1856:1864	CONCLUSIONS We showed that chronic, voluntary EtOH consumption induced liver injury and endotoxemia together with dysbiotic changes in sP rats.
30320890	7	85	theme	serum	1134:1138	arg1	transaminases					1140:1152	serum transaminases	1134:1152	serum transaminases	1134:1152	RESULTS Rats in Group Et developed hepatic steatosis and elevated serum transaminases and endotoxin/lipopolysaccharide (LPS) levels but no other liver pathological changes (i.e., necrosis/inflammation) or systemic inflammation.
30320890	11	86	theme	Ortholog	1671:1678	arg1	profile					1680:1686	The KEGG Ortholog profile	1662:1686	The KEGG Ortholog profile	1662:1686	The KEGG Ortholog profile revealed that IM functional modules, such as biosynthesis, transport, and export of LPS, were also enriched in Group Et rats at T3.
30320890	4	87	theme	male	529:532	arg1	rats					543:546	male adult sP rats	529:546	male adult sP rats	529:546	METHODS Independent groups of male adult sP rats were exposed to the standard, home-cage 2-bottle "EtOH (10% v/v) versus water" choice regimen with unlimited access for 24 h/d (Group Et) for 3 (T1), 6 (T2), and 12 (T3) consecutive months.
30320890	3	88	theme	Sardinian	403:411	arg1	alcohol-preferring					413:430	Sardinian alcohol-preferring	403:430	selectively bred Sardinian alcohol-preferring (sP) rats	386:440	We investigated this issue using selectively bred Sardinian alcohol-preferring (sP) rats, a validated animal model of excessive EtOH consumption.
30320890	3	88	theme	Sardinian	403:411	arg1	sP					433:434	sP	433:434	sP	433:434	We investigated this issue using selectively bred Sardinian alcohol-preferring (sP) rats, a validated animal model of excessive EtOH consumption.
30320890	6	89	used	used	892:895	arg2	we					889:890	we	889:890	we	889:890	We obtained samples from each rat at the end of each experimental time, and we used blood and colon tissues for intestinal barrier integrity and/or liver pathology assessments and used stool samples for IM analysis with 16S ribosomal RNA gene sequencing.
30320890	5	90	theme	matched-age	781:791	arg1	rats					807:810	matched-age EtOH-naïve sP rats	781:810	matched-age EtOH-naïve sP rats	781:810	Control groups (Group Ct) were composed of matched-age EtOH-naïve sP rats.
30320890	6	91	theme	gene	1051:1054	arg1	sequencing					1056:1065	ribosomal RNA gene sequencing	1037:1065	ribosomal RNA gene sequencing	1037:1065	We obtained samples from each rat at the end of each experimental time, and we used blood and colon tissues for intestinal barrier integrity and/or liver pathology assessments and used stool samples for IM analysis with 16S ribosomal RNA gene sequencing.
30320890	6	92	theme	time	879:882	arg1	end					854:856	the end	850:856	the end of each experimental time	850:882	We obtained samples from each rat at the end of each experimental time, and we used blood and colon tissues for intestinal barrier integrity and/or liver pathology assessments and used stool samples for IM analysis with 16S ribosomal RNA gene sequencing.
30320890	4	93	theme	sP	540:541	arg1	rats					543:546	male adult sP rats	529:546	male adult sP rats	529:546	METHODS Independent groups of male adult sP rats were exposed to the standard, home-cage 2-bottle "EtOH (10% v/v) versus water" choice regimen with unlimited access for 24 h/d (Group Et) for 3 (T1), 6 (T2), and 12 (T3) consecutive months.
30320890	8	94	from	rats	1471:1474	arg1	Group					1479:1483	Group Ct	1479:1486	Group Ct	1479:1486	While we did not find any apparent alteration of the intestinal colonic mucosa, we found that rats in Group Et exhibited significant changes in IM composition compared to the rats in Group Ct.
30320890	2	95	theme	gut-liver	330:338	arg1	homeostasis					340:350	gut-liver homeostasis	330:350	gut-liver homeostasis	330:350	However, it is unclear how changes in the intestinal microbiota (IM) relate to ethanol (EtOH)-induced disruption of gut-liver homeostasis.
30320890	2	96	from	changes	241:247	arg1	microbiota					267:276	the intestinal microbiota	252:276	the intestinal microbiota (IM)	252:281	However, it is unclear how changes in the intestinal microbiota (IM) relate to ethanol (EtOH)-induced disruption of gut-liver homeostasis.
30320890	2	96	from	changes	241:247	arg1	IM					279:280	IM	279:280	IM	279:280	However, it is unclear how changes in the intestinal microbiota (IM) relate to ethanol (EtOH)-induced disruption of gut-liver homeostasis.
30320890	7	97	theme	hepatic	1103:1109	arg1	steatosis					1111:1119	hepatic steatosis	1103:1119	hepatic steatosis	1103:1119	RESULTS Rats in Group Et developed hepatic steatosis and elevated serum transaminases and endotoxin/lipopolysaccharide (LPS) levels but no other liver pathological changes (i.e., necrosis/inflammation) or systemic inflammation.
30320890	11	98	theme	IM	1702:1703	arg1	transport					1747:1755	transport	1747:1755	transport	1747:1755	The KEGG Ortholog profile revealed that IM functional modules, such as biosynthesis, transport, and export of LPS, were also enriched in Group Et rats at T3.
30320890	11	98	theme	IM	1702:1703	arg1	export					1762:1767	export	1762:1767	export	1762:1767	The KEGG Ortholog profile revealed that IM functional modules, such as biosynthesis, transport, and export of LPS, were also enriched in Group Et rats at T3.
30320890	11	98	theme	IM	1702:1703	arg1	biosynthesis					1733:1744	biosynthesis	1733:1744	biosynthesis	1733:1744	The KEGG Ortholog profile revealed that IM functional modules, such as biosynthesis, transport, and export of LPS, were also enriched in Group Et rats at T3.
30320890	11	98	theme	IM	1702:1703	arg1	modules					1716:1722	IM functional modules	1702:1722	IM functional modules	1702:1722	The KEGG Ortholog profile revealed that IM functional modules, such as biosynthesis, transport, and export of LPS, were also enriched in Group Et rats at T3.
30320890	4	99	theme	6	698:698	arg1	months					730:735	3 (T1), 6 (T2), and 12 (T3) consecutive months	690:735	3 (T1), 6 (T2), and 12 (T3) consecutive months	690:735	METHODS Independent groups of male adult sP rats were exposed to the standard, home-cage 2-bottle "EtOH (10% v/v) versus water" choice regimen with unlimited access for 24 h/d (Group Et) for 3 (T1), 6 (T2), and 12 (T3) consecutive months.
30320890	12	100	theme	sP	1955:1956	arg1	rats					1958:1961	sP rats	1955:1961	sP rats	1955:1961	CONCLUSIONS We showed that chronic, voluntary EtOH consumption induced liver injury and endotoxemia together with dysbiotic changes in sP rats.
30320890	6	101	theme	RNA	1047:1049	arg1	sequencing					1056:1065	ribosomal RNA gene sequencing	1037:1065	ribosomal RNA gene sequencing	1037:1065	We obtained samples from each rat at the end of each experimental time, and we used blood and colon tissues for intestinal barrier integrity and/or liver pathology assessments and used stool samples for IM analysis with 16S ribosomal RNA gene sequencing.
30320890	6	102	theme	intestinal	925:934	arg1	integrity					944:952	intestinal barrier integrity	925:952	intestinal barrier integrity	925:952	We obtained samples from each rat at the end of each experimental time, and we used blood and colon tissues for intestinal barrier integrity and/or liver pathology assessments and used stool samples for IM analysis with 16S ribosomal RNA gene sequencing.
30320890	8	103	theme	significant	1417:1427	arg1	changes					1429:1435	significant changes	1417:1435	significant changes in IM composition	1417:1453	While we did not find any apparent alteration of the intestinal colonic mucosa, we found that rats in Group Et exhibited significant changes in IM composition compared to the rats in Group Ct.
30320890	3	104	theme	validated	445:453	arg1	model					462:466	a validated animal model	443:466	a validated animal model of excessive EtOH consumption	443:496	We investigated this issue using selectively bred Sardinian alcohol-preferring (sP) rats, a validated animal model of excessive EtOH consumption.
30320890	3	104	theme	validated	445:453	arg1	rats					437:440	selectively bred Sardinian alcohol-preferring (sP) rats	386:440	selectively bred Sardinian alcohol-preferring (sP) rats	386:440	We investigated this issue using selectively bred Sardinian alcohol-preferring (sP) rats, a validated animal model of excessive EtOH consumption.
30320890	7	105	theme	RESULTS	1068:1074	arg1	Rats					1076:1079	RESULTS Rats	1068:1079	RESULTS Rats in Group Et	1068:1091	RESULTS Rats in Group Et developed hepatic steatosis and elevated serum transaminases and endotoxin/lipopolysaccharide (LPS) levels but no other liver pathological changes (i.e., necrosis/inflammation) or systemic inflammation.
30320890	13	106	theme	prevention	2024:2033	arg1	knowledge					2007:2015	our knowledge	2003:2015	our knowledge of the prevention and treatment of EtOH-related diseases	2003:2072	This work sets the stage for improving our knowledge of the prevention and treatment of EtOH-related diseases.
30320890	3	107	theme	consumption	486:496	arg1	model					462:466	a validated animal model	443:466	a validated animal model of excessive EtOH consumption	443:496	We investigated this issue using selectively bred Sardinian alcohol-preferring (sP) rats, a validated animal model of excessive EtOH consumption.
30320890	3	107	theme	consumption	486:496	arg1	rats					437:440	selectively bred Sardinian alcohol-preferring (sP) rats	386:440	selectively bred Sardinian alcohol-preferring (sP) rats	386:440	We investigated this issue using selectively bred Sardinian alcohol-preferring (sP) rats, a validated animal model of excessive EtOH consumption.
31790895	9	0	theme	CONCLUSION	1684:1693	arg1	QHD					1695:1697	CONCLUSION QHD	1684:1697	CONCLUSION QHD	1684:1697	CONCLUSION QHD inhibits LPS gut-leakage in NASH, which is associated with downregulation of intestinal MAPK pathway.
31790895	8	1	theme	protein	1575:1581	arg1	MAPK					1591:1594	MAPK	1591:1594	MAPK	1591:1594	The validated data of phosphorylated proteins suggested that mitogen-activated protein kinase (MAPK) pathway is predominantly responsible for the colonic tight junction regulation by QHD.
31790895	8	1	theme	protein	1575:1581	arg1	kinase					1583:1588	mitogen-activated protein kinase	1557:1588	mitogen-activated protein kinase (MAPK) pathway	1557:1603	The validated data of phosphorylated proteins suggested that mitogen-activated protein kinase (MAPK) pathway is predominantly responsible for the colonic tight junction regulation by QHD.
31790895	6	2	theme	responsible	1058:1068	arg1	pathway					1050:1056	the potential signalling pathway	1025:1056	the potential signalling pathway responsible for tight junction regulation	1025:1098	To identify the potential signalling pathway responsible for tight junction regulation, the colonic phosphoprotein profile is screened via the Phospho Explorer Antibody Array and verified in NASH, intestinal barrier dysfunctional mouse and Caco-2 cells.
31790895	8	3	theme	mitogen-activated	1557:1573	arg1	MAPK					1591:1594	MAPK	1591:1594	MAPK	1591:1594	The validated data of phosphorylated proteins suggested that mitogen-activated protein kinase (MAPK) pathway is predominantly responsible for the colonic tight junction regulation by QHD.
31790895	8	3	theme	mitogen-activated	1557:1573	arg1	kinase					1583:1588	mitogen-activated protein kinase	1557:1588	mitogen-activated protein kinase (MAPK) pathway	1557:1603	The validated data of phosphorylated proteins suggested that mitogen-activated protein kinase (MAPK) pathway is predominantly responsible for the colonic tight junction regulation by QHD.
31790895	1	4	theme	liver	280:284	arg1	disease					286:292	non-alcoholic fatty liver disease	260:292	non-alcoholic fatty liver disease (NAFLD)	260:300	BACKGROUND Gut microbiota is increasingly recognized as the key participant in the pathogenesis of non-alcoholic fatty liver disease (NAFLD) by translocation of its products, such as lipopolysaccharide (LPS), via the dysfunctional intestinal barrier.
31790895	1	4	theme	liver	280:284	arg1	NAFLD					295:299	NAFLD	295:299	NAFLD	295:299	BACKGROUND Gut microbiota is increasingly recognized as the key participant in the pathogenesis of non-alcoholic fatty liver disease (NAFLD) by translocation of its products, such as lipopolysaccharide (LPS), via the dysfunctional intestinal barrier.
31790895	4	5	theme	METHODS	828:834	arg1	Effects					836:842	METHODS Effects	828:842	METHODS Effects of QHD on gut microbioa and intestinal barrier	828:889	METHODS Effects of QHD on gut microbioa and intestinal barrier were evaluated in NASH induced by HFD in mice.
31790895	9	6	theme	LPS	1708:1710	arg1	gut-leakage					1712:1722	LPS gut-leakage	1708:1722	LPS gut-leakage	1708:1722	CONCLUSION QHD inhibits LPS gut-leakage in NASH, which is associated with downregulation of intestinal MAPK pathway.
31790895	8	7	theme	proteins	1533:1540	arg1	data					1510:1513	The validated data	1496:1513	The validated data of phosphorylated proteins	1496:1540	The validated data of phosphorylated proteins suggested that mitogen-activated protein kinase (MAPK) pathway is predominantly responsible for the colonic tight junction regulation by QHD.
31790895	1	8	theme	disease	286:292	arg1	pathogenesis					244:255	the pathogenesis	240:255	the pathogenesis of non-alcoholic fatty liver disease (NAFLD)	240:300	BACKGROUND Gut microbiota is increasingly recognized as the key participant in the pathogenesis of non-alcoholic fatty liver disease (NAFLD) by translocation of its products, such as lipopolysaccharide (LPS), via the dysfunctional intestinal barrier.
31790895	4	9	from	Effects	836:842	arg1	microbioa					858:866	gut microbioa	854:866	gut microbioa	854:866	METHODS Effects of QHD on gut microbioa and intestinal barrier were evaluated in NASH induced by HFD in mice.
31790895	4	9	from	Effects	836:842	arg1	barrier					883:889	intestinal barrier	872:889	intestinal barrier	872:889	METHODS Effects of QHD on gut microbioa and intestinal barrier were evaluated in NASH induced by HFD in mice.
31790895	3	10	from	QHD	815:817	arg1	NASH					822:825	NASH	822:825	NASH	822:825	PURPOSE To investigate the mechanism of LPS gut-leakage inhibition by QHD in NASH.
31790895	6	11	theme	barrier	1221:1227	arg1	mouse					1243:1247	intestinal barrier dysfunctional mouse	1210:1247	intestinal barrier dysfunctional mouse	1210:1247	To identify the potential signalling pathway responsible for tight junction regulation, the colonic phosphoprotein profile is screened via the Phospho Explorer Antibody Array and verified in NASH, intestinal barrier dysfunctional mouse and Caco-2 cells.
31790895	6	12	theme	Phospho	1156:1162	arg1	Array					1182:1186	the Phospho Explorer Antibody Array	1152:1186	the Phospho Explorer Antibody Array	1152:1186	To identify the potential signalling pathway responsible for tight junction regulation, the colonic phosphoprotein profile is screened via the Phospho Explorer Antibody Array and verified in NASH, intestinal barrier dysfunctional mouse and Caco-2 cells.
31790895	3	13	theme	gut-leakage	789:799	arg1	inhibition					801:810	LPS gut-leakage inhibition	785:810	LPS gut-leakage inhibition by QHD in NASH	785:825	PURPOSE To investigate the mechanism of LPS gut-leakage inhibition by QHD in NASH.
31790895	7	14	theme	Gram-negative	1472:1484	arg1	bacteria					1486:1493	identified Gram-negative bacteria	1461:1493	identified Gram-negative bacteria	1461:1493	RESULTS QHD ameliorates NASH accompanied with regulating the gut microbiota composition, protecting intestinal tight junctions and inhibiting LPS gut-leakage without decreasing the abundance of identified Gram-negative bacteria.
31790895	4	15	theme	QHD	847:849	arg1	Effects					836:842	METHODS Effects	828:842	METHODS Effects of QHD on gut microbioa and intestinal barrier	828:889	METHODS Effects of QHD on gut microbioa and intestinal barrier were evaluated in NASH induced by HFD in mice.
31790895	3	16	theme	inhibition	801:810	arg1	mechanism					772:780	the mechanism	768:780	the mechanism of LPS gut-leakage inhibition by QHD in NASH	768:825	PURPOSE To investigate the mechanism of LPS gut-leakage inhibition by QHD in NASH.
31790895	0	17	theme	mitogen-activated	119:135	arg1	kinase					145:150	intestinal mitogen-activated protein kinase	108:150	the intestinal mitogen-activated protein kinase pathway	104:158	Amelioration of non-alcoholic steatohepatitis by Qushi Huayu decoction is associated with inhibition of the intestinal mitogen-activated protein kinase pathway.
31790895	7	18	theme	bacteria	1486:1493	arg1	abundance					1448:1456	the abundance	1444:1456	the abundance of identified Gram-negative bacteria	1444:1493	RESULTS QHD ameliorates NASH accompanied with regulating the gut microbiota composition, protecting intestinal tight junctions and inhibiting LPS gut-leakage without decreasing the abundance of identified Gram-negative bacteria.
31790895	2	19	theme	LPS	729:731	arg1	signalling					733:742	hepatic LPS signalling	721:742	hepatic LPS signalling	721:742	Qushi Huayu decoction (QHD), a traditional Chinese medicine, is developed specially for NAFLD and used in clinic in China for more than a decade and previously found to ameliorate non-alcoholic steatohepatitis (NASH) induced by high-fat diet (HFD) in mice accompanied with inhibited metabolic endotoxemia and hepatic LPS signalling.
31790895	6	20	theme	signalling	1039:1048	arg1	pathway					1050:1056	the potential signalling pathway	1025:1056	the potential signalling pathway responsible for tight junction regulation	1025:1098	To identify the potential signalling pathway responsible for tight junction regulation, the colonic phosphoprotein profile is screened via the Phospho Explorer Antibody Array and verified in NASH, intestinal barrier dysfunctional mouse and Caco-2 cells.
31790895	0	21	theme	intestinal	108:117	arg1	kinase					145:150	intestinal mitogen-activated protein kinase	108:150	the intestinal mitogen-activated protein kinase pathway	104:158	Amelioration of non-alcoholic steatohepatitis by Qushi Huayu decoction is associated with inhibition of the intestinal mitogen-activated protein kinase pathway.
31790895	8	22	theme	kinase	1583:1588	arg1	pathway					1597:1603	mitogen-activated protein kinase (MAPK) pathway	1557:1603	mitogen-activated protein kinase (MAPK) pathway	1557:1603	The validated data of phosphorylated proteins suggested that mitogen-activated protein kinase (MAPK) pathway is predominantly responsible for the colonic tight junction regulation by QHD.
31790895	6	23	theme	dysfunctional	1229:1241	arg1	mouse					1243:1247	intestinal barrier dysfunctional mouse	1210:1247	intestinal barrier dysfunctional mouse	1210:1247	To identify the potential signalling pathway responsible for tight junction regulation, the colonic phosphoprotein profile is screened via the Phospho Explorer Antibody Array and verified in NASH, intestinal barrier dysfunctional mouse and Caco-2 cells.
31790895	3	24	theme	LPS	785:787	arg1	inhibition					801:810	LPS gut-leakage inhibition	785:810	LPS gut-leakage inhibition by QHD in NASH	785:825	PURPOSE To investigate the mechanism of LPS gut-leakage inhibition by QHD in NASH.
31790895	2	25	theme	hepatic	721:727	arg1	signalling					733:742	hepatic LPS signalling	721:742	hepatic LPS signalling	721:742	Qushi Huayu decoction (QHD), a traditional Chinese medicine, is developed specially for NAFLD and used in clinic in China for more than a decade and previously found to ameliorate non-alcoholic steatohepatitis (NASH) induced by high-fat diet (HFD) in mice accompanied with inhibited metabolic endotoxemia and hepatic LPS signalling.
31790895	6	26	theme	potential	1029:1037	arg1	pathway					1050:1056	the potential signalling pathway	1025:1056	the potential signalling pathway responsible for tight junction regulation	1025:1098	To identify the potential signalling pathway responsible for tight junction regulation, the colonic phosphoprotein profile is screened via the Phospho Explorer Antibody Array and verified in NASH, intestinal barrier dysfunctional mouse and Caco-2 cells.
31790895	7	27	theme	identified	1461:1470	arg1	bacteria					1486:1493	identified Gram-negative bacteria	1461:1493	identified Gram-negative bacteria	1461:1493	RESULTS QHD ameliorates NASH accompanied with regulating the gut microbiota composition, protecting intestinal tight junctions and inhibiting LPS gut-leakage without decreasing the abundance of identified Gram-negative bacteria.
31790895	2	28	theme	non-alcoholic	592:604	arg1	NASH					623:626	NASH	623:626	NASH	623:626	Qushi Huayu decoction (QHD), a traditional Chinese medicine, is developed specially for NAFLD and used in clinic in China for more than a decade and previously found to ameliorate non-alcoholic steatohepatitis (NASH) induced by high-fat diet (HFD) in mice accompanied with inhibited metabolic endotoxemia and hepatic LPS signalling.
31790895	2	28	theme	non-alcoholic	592:604	arg1	steatohepatitis					606:620	non-alcoholic steatohepatitis	592:620	non-alcoholic steatohepatitis (NASH) induced by high-fat diet (HFD) in mice accompanied with inhibited metabolic endotoxemia and hepatic LPS signalling	592:742	Qushi Huayu decoction (QHD), a traditional Chinese medicine, is developed specially for NAFLD and used in clinic in China for more than a decade and previously found to ameliorate non-alcoholic steatohepatitis (NASH) induced by high-fat diet (HFD) in mice accompanied with inhibited metabolic endotoxemia and hepatic LPS signalling.
31790895	9	29	theme	MAPK	1787:1790	arg1	pathway					1792:1798	intestinal MAPK pathway	1776:1798	intestinal MAPK pathway	1776:1798	CONCLUSION QHD inhibits LPS gut-leakage in NASH, which is associated with downregulation of intestinal MAPK pathway.
31790895	7	30	theme	LPS	1409:1411	arg1	gut-leakage					1413:1423	LPS gut-leakage	1409:1423	LPS gut-leakage	1409:1423	RESULTS QHD ameliorates NASH accompanied with regulating the gut microbiota composition, protecting intestinal tight junctions and inhibiting LPS gut-leakage without decreasing the abundance of identified Gram-negative bacteria.
31790895	8	31	theme	tight	1650:1654	arg1	regulation					1665:1674	the colonic tight junction regulation	1638:1674	the colonic tight junction regulation by QHD	1638:1681	The validated data of phosphorylated proteins suggested that mitogen-activated protein kinase (MAPK) pathway is predominantly responsible for the colonic tight junction regulation by QHD.
31790895	6	32	theme	intestinal	1210:1219	arg1	mouse					1243:1247	intestinal barrier dysfunctional mouse	1210:1247	intestinal barrier dysfunctional mouse	1210:1247	To identify the potential signalling pathway responsible for tight junction regulation, the colonic phosphoprotein profile is screened via the Phospho Explorer Antibody Array and verified in NASH, intestinal barrier dysfunctional mouse and Caco-2 cells.
31790895	5	33	theme	microbiota	989:998	arg1	composition					1000:1010	the gut microbiota composition	981:1010	the gut microbiota composition	981:1010	16S rRNA sequencing is employed to analyse the gut microbiota composition.
31790895	7	34	theme	RESULTS	1267:1273	arg1	QHD					1275:1277	RESULTS QHD	1267:1277	RESULTS QHD	1267:1277	RESULTS QHD ameliorates NASH accompanied with regulating the gut microbiota composition, protecting intestinal tight junctions and inhibiting LPS gut-leakage without decreasing the abundance of identified Gram-negative bacteria.
31790895	0	35	theme	steatohepatitis	30:44	arg1	Amelioration					0:11	Amelioration	0:11	Amelioration of non-alcoholic steatohepatitis by Qushi Huayu decoction	0:69	Amelioration of non-alcoholic steatohepatitis by Qushi Huayu decoction is associated with inhibition of the intestinal mitogen-activated protein kinase pathway.
31790895	7	36	theme	gut	1328:1330	arg1	composition					1343:1353	the gut microbiota composition	1324:1353	the gut microbiota composition	1324:1353	RESULTS QHD ameliorates NASH accompanied with regulating the gut microbiota composition, protecting intestinal tight junctions and inhibiting LPS gut-leakage without decreasing the abundance of identified Gram-negative bacteria.
31790895	0	37	theme	kinase	145:150	arg1	pathway					152:158	the intestinal mitogen-activated protein kinase pathway	104:158	the intestinal mitogen-activated protein kinase pathway	104:158	Amelioration of non-alcoholic steatohepatitis by Qushi Huayu decoction is associated with inhibition of the intestinal mitogen-activated protein kinase pathway.
31790895	2	38	theme	high-fat	640:647	arg1	diet					649:652	high-fat diet	640:652	high-fat diet (HFD)	640:658	Qushi Huayu decoction (QHD), a traditional Chinese medicine, is developed specially for NAFLD and used in clinic in China for more than a decade and previously found to ameliorate non-alcoholic steatohepatitis (NASH) induced by high-fat diet (HFD) in mice accompanied with inhibited metabolic endotoxemia and hepatic LPS signalling.
31790895	2	38	theme	high-fat	640:647	arg1	HFD					655:657	HFD	655:657	HFD	655:657	Qushi Huayu decoction (QHD), a traditional Chinese medicine, is developed specially for NAFLD and used in clinic in China for more than a decade and previously found to ameliorate non-alcoholic steatohepatitis (NASH) induced by high-fat diet (HFD) in mice accompanied with inhibited metabolic endotoxemia and hepatic LPS signalling.
31790895	6	39	theme	phosphoprotein	1113:1126	arg1	profile					1128:1134	the colonic phosphoprotein profile	1101:1134	the colonic phosphoprotein profile	1101:1134	To identify the potential signalling pathway responsible for tight junction regulation, the colonic phosphoprotein profile is screened via the Phospho Explorer Antibody Array and verified in NASH, intestinal barrier dysfunctional mouse and Caco-2 cells.
31790895	1	40	theme	key	221:223	arg1	participant					225:235	the key participant	217:235	the key participant in the pathogenesis of non-alcoholic fatty liver disease (NAFLD)	217:300	BACKGROUND Gut microbiota is increasingly recognized as the key participant in the pathogenesis of non-alcoholic fatty liver disease (NAFLD) by translocation of its products, such as lipopolysaccharide (LPS), via the dysfunctional intestinal barrier.
31790895	1	40	theme	key	221:223	arg1	microbiota					176:185	BACKGROUND Gut microbiota	161:185	BACKGROUND Gut microbiota	161:185	BACKGROUND Gut microbiota is increasingly recognized as the key participant in the pathogenesis of non-alcoholic fatty liver disease (NAFLD) by translocation of its products, such as lipopolysaccharide (LPS), via the dysfunctional intestinal barrier.
31790895	0	41	theme	non-alcoholic	16:28	arg1	steatohepatitis					30:44	non-alcoholic steatohepatitis	16:44	non-alcoholic steatohepatitis	16:44	Amelioration of non-alcoholic steatohepatitis by Qushi Huayu decoction is associated with inhibition of the intestinal mitogen-activated protein kinase pathway.
31790895	9	42	from	gut-leakage	1712:1722	arg1	NASH					1727:1730	NASH	1727:1730	NASH	1727:1730	CONCLUSION QHD inhibits LPS gut-leakage in NASH, which is associated with downregulation of intestinal MAPK pathway.
31790895	0	43	theme	protein	137:143	arg1	kinase					145:150	intestinal mitogen-activated protein kinase	108:150	the intestinal mitogen-activated protein kinase pathway	104:158	Amelioration of non-alcoholic steatohepatitis by Qushi Huayu decoction is associated with inhibition of the intestinal mitogen-activated protein kinase pathway.
31790895	1	44	theme	dysfunctional	378:390	arg1	barrier					403:409	the dysfunctional intestinal barrier	374:409	the dysfunctional intestinal barrier	374:409	BACKGROUND Gut microbiota is increasingly recognized as the key participant in the pathogenesis of non-alcoholic fatty liver disease (NAFLD) by translocation of its products, such as lipopolysaccharide (LPS), via the dysfunctional intestinal barrier.
31790895	6	45	theme	colonic	1105:1111	arg1	profile					1128:1134	the colonic phosphoprotein profile	1101:1134	the colonic phosphoprotein profile	1101:1134	To identify the potential signalling pathway responsible for tight junction regulation, the colonic phosphoprotein profile is screened via the Phospho Explorer Antibody Array and verified in NASH, intestinal barrier dysfunctional mouse and Caco-2 cells.
31790895	6	46	theme	tight	1074:1078	arg1	regulation					1089:1098	tight junction regulation	1074:1098	tight junction regulation	1074:1098	To identify the potential signalling pathway responsible for tight junction regulation, the colonic phosphoprotein profile is screened via the Phospho Explorer Antibody Array and verified in NASH, intestinal barrier dysfunctional mouse and Caco-2 cells.
31790895	0	47	theme	Qushi	49:53	arg1	decoction					61:69	Qushi Huayu decoction	49:69	Qushi Huayu decoction	49:69	Amelioration of non-alcoholic steatohepatitis by Qushi Huayu decoction is associated with inhibition of the intestinal mitogen-activated protein kinase pathway.
31790895	1	48	theme	intestinal	392:401	arg1	barrier					403:409	the dysfunctional intestinal barrier	374:409	the dysfunctional intestinal barrier	374:409	BACKGROUND Gut microbiota is increasingly recognized as the key participant in the pathogenesis of non-alcoholic fatty liver disease (NAFLD) by translocation of its products, such as lipopolysaccharide (LPS), via the dysfunctional intestinal barrier.
31790895	7	49	theme	tight	1378:1382	arg1	junctions					1384:1392	intestinal tight junctions	1367:1392	intestinal tight junctions	1367:1392	RESULTS QHD ameliorates NASH accompanied with regulating the gut microbiota composition, protecting intestinal tight junctions and inhibiting LPS gut-leakage without decreasing the abundance of identified Gram-negative bacteria.
31790895	2	50	theme	Huayu	418:422	arg1	medicine					463:470	a traditional Chinese medicine	441:470	a traditional Chinese medicine	441:470	Qushi Huayu decoction (QHD), a traditional Chinese medicine, is developed specially for NAFLD and used in clinic in China for more than a decade and previously found to ameliorate non-alcoholic steatohepatitis (NASH) induced by high-fat diet (HFD) in mice accompanied with inhibited metabolic endotoxemia and hepatic LPS signalling.
31790895	2	50	theme	Huayu	418:422	arg1	QHD					435:437	QHD	435:437	QHD	435:437	Qushi Huayu decoction (QHD), a traditional Chinese medicine, is developed specially for NAFLD and used in clinic in China for more than a decade and previously found to ameliorate non-alcoholic steatohepatitis (NASH) induced by high-fat diet (HFD) in mice accompanied with inhibited metabolic endotoxemia and hepatic LPS signalling.
31790895	2	50	theme	Huayu	418:422	arg1	decoction					424:432	Qushi Huayu decoction	412:432	Qushi Huayu decoction (QHD)	412:438	Qushi Huayu decoction (QHD), a traditional Chinese medicine, is developed specially for NAFLD and used in clinic in China for more than a decade and previously found to ameliorate non-alcoholic steatohepatitis (NASH) induced by high-fat diet (HFD) in mice accompanied with inhibited metabolic endotoxemia and hepatic LPS signalling.
31790895	0	51	theme	pathway	152:158	arg1	inhibition					90:99	inhibition	90:99	inhibition of the intestinal mitogen-activated protein kinase pathway	90:158	Amelioration of non-alcoholic steatohepatitis by Qushi Huayu decoction is associated with inhibition of the intestinal mitogen-activated protein kinase pathway.
31790895	2	52	used	used	510:513	arg2	QHD					435:437	QHD	435:437	QHD	435:437	Qushi Huayu decoction (QHD), a traditional Chinese medicine, is developed specially for NAFLD and used in clinic in China for more than a decade and previously found to ameliorate non-alcoholic steatohepatitis (NASH) induced by high-fat diet (HFD) in mice accompanied with inhibited metabolic endotoxemia and hepatic LPS signalling.
31790895	2	52	used	used	510:513	arg2	decoction					424:432	Qushi Huayu decoction	412:432	Qushi Huayu decoction (QHD)	412:438	Qushi Huayu decoction (QHD), a traditional Chinese medicine, is developed specially for NAFLD and used in clinic in China for more than a decade and previously found to ameliorate non-alcoholic steatohepatitis (NASH) induced by high-fat diet (HFD) in mice accompanied with inhibited metabolic endotoxemia and hepatic LPS signalling.
31790895	2	52	used	used	510:513	arg2	medicine					463:470	a traditional Chinese medicine	441:470	a traditional Chinese medicine	441:470	Qushi Huayu decoction (QHD), a traditional Chinese medicine, is developed specially for NAFLD and used in clinic in China for more than a decade and previously found to ameliorate non-alcoholic steatohepatitis (NASH) induced by high-fat diet (HFD) in mice accompanied with inhibited metabolic endotoxemia and hepatic LPS signalling.
31790895	8	53	theme	junction	1656:1663	arg1	regulation					1665:1674	the colonic tight junction regulation	1638:1674	the colonic tight junction regulation by QHD	1638:1681	The validated data of phosphorylated proteins suggested that mitogen-activated protein kinase (MAPK) pathway is predominantly responsible for the colonic tight junction regulation by QHD.
31790895	7	54	theme	intestinal	1367:1376	arg1	junctions					1384:1392	intestinal tight junctions	1367:1392	intestinal tight junctions	1367:1392	RESULTS QHD ameliorates NASH accompanied with regulating the gut microbiota composition, protecting intestinal tight junctions and inhibiting LPS gut-leakage without decreasing the abundance of identified Gram-negative bacteria.
31790895	2	55	theme	Qushi	412:416	arg1	medicine					463:470	a traditional Chinese medicine	441:470	a traditional Chinese medicine	441:470	Qushi Huayu decoction (QHD), a traditional Chinese medicine, is developed specially for NAFLD and used in clinic in China for more than a decade and previously found to ameliorate non-alcoholic steatohepatitis (NASH) induced by high-fat diet (HFD) in mice accompanied with inhibited metabolic endotoxemia and hepatic LPS signalling.
31790895	2	55	theme	Qushi	412:416	arg1	QHD					435:437	QHD	435:437	QHD	435:437	Qushi Huayu decoction (QHD), a traditional Chinese medicine, is developed specially for NAFLD and used in clinic in China for more than a decade and previously found to ameliorate non-alcoholic steatohepatitis (NASH) induced by high-fat diet (HFD) in mice accompanied with inhibited metabolic endotoxemia and hepatic LPS signalling.
31790895	2	55	theme	Qushi	412:416	arg1	decoction					424:432	Qushi Huayu decoction	412:432	Qushi Huayu decoction (QHD)	412:438	Qushi Huayu decoction (QHD), a traditional Chinese medicine, is developed specially for NAFLD and used in clinic in China for more than a decade and previously found to ameliorate non-alcoholic steatohepatitis (NASH) induced by high-fat diet (HFD) in mice accompanied with inhibited metabolic endotoxemia and hepatic LPS signalling.
31790895	2	56	theme	Chinese	455:461	arg1	decoction					424:432	Qushi Huayu decoction	412:432	Qushi Huayu decoction (QHD)	412:438	Qushi Huayu decoction (QHD), a traditional Chinese medicine, is developed specially for NAFLD and used in clinic in China for more than a decade and previously found to ameliorate non-alcoholic steatohepatitis (NASH) induced by high-fat diet (HFD) in mice accompanied with inhibited metabolic endotoxemia and hepatic LPS signalling.
31790895	2	56	theme	Chinese	455:461	arg1	medicine					463:470	a traditional Chinese medicine	441:470	a traditional Chinese medicine	441:470	Qushi Huayu decoction (QHD), a traditional Chinese medicine, is developed specially for NAFLD and used in clinic in China for more than a decade and previously found to ameliorate non-alcoholic steatohepatitis (NASH) induced by high-fat diet (HFD) in mice accompanied with inhibited metabolic endotoxemia and hepatic LPS signalling.
31790895	1	57	theme	products	326:333	arg1	translocation					305:317	translocation	305:317	translocation of its products, such as lipopolysaccharide (LPS), via the dysfunctional intestinal barrier	305:409	BACKGROUND Gut microbiota is increasingly recognized as the key participant in the pathogenesis of non-alcoholic fatty liver disease (NAFLD) by translocation of its products, such as lipopolysaccharide (LPS), via the dysfunctional intestinal barrier.
31790895	9	58	theme	intestinal	1776:1785	arg1	pathway					1792:1798	intestinal MAPK pathway	1776:1798	intestinal MAPK pathway	1776:1798	CONCLUSION QHD inhibits LPS gut-leakage in NASH, which is associated with downregulation of intestinal MAPK pathway.
31790895	0	59	theme	Huayu	55:59	arg1	decoction					61:69	Qushi Huayu decoction	49:69	Qushi Huayu decoction	49:69	Amelioration of non-alcoholic steatohepatitis by Qushi Huayu decoction is associated with inhibition of the intestinal mitogen-activated protein kinase pathway.
31790895	4	60	theme	gut	854:856	arg1	microbioa					858:866	gut microbioa	854:866	gut microbioa	854:866	METHODS Effects of QHD on gut microbioa and intestinal barrier were evaluated in NASH induced by HFD in mice.
31790895	2	61	theme	traditional	443:453	arg1	decoction					424:432	Qushi Huayu decoction	412:432	Qushi Huayu decoction (QHD)	412:438	Qushi Huayu decoction (QHD), a traditional Chinese medicine, is developed specially for NAFLD and used in clinic in China for more than a decade and previously found to ameliorate non-alcoholic steatohepatitis (NASH) induced by high-fat diet (HFD) in mice accompanied with inhibited metabolic endotoxemia and hepatic LPS signalling.
31790895	2	61	theme	traditional	443:453	arg1	medicine					463:470	a traditional Chinese medicine	441:470	a traditional Chinese medicine	441:470	Qushi Huayu decoction (QHD), a traditional Chinese medicine, is developed specially for NAFLD and used in clinic in China for more than a decade and previously found to ameliorate non-alcoholic steatohepatitis (NASH) induced by high-fat diet (HFD) in mice accompanied with inhibited metabolic endotoxemia and hepatic LPS signalling.
31790895	5	62	theme	gut	985:987	arg1	composition					1000:1010	the gut microbiota composition	981:1010	the gut microbiota composition	981:1010	16S rRNA sequencing is employed to analyse the gut microbiota composition.
31790895	6	63	theme	Caco-2	1253:1258	arg1	cells					1260:1264	Caco-2 cells	1253:1264	Caco-2 cells	1253:1264	To identify the potential signalling pathway responsible for tight junction regulation, the colonic phosphoprotein profile is screened via the Phospho Explorer Antibody Array and verified in NASH, intestinal barrier dysfunctional mouse and Caco-2 cells.
31790895	7	64	theme	microbiota	1332:1341	arg1	composition					1343:1353	the gut microbiota composition	1324:1353	the gut microbiota composition	1324:1353	RESULTS QHD ameliorates NASH accompanied with regulating the gut microbiota composition, protecting intestinal tight junctions and inhibiting LPS gut-leakage without decreasing the abundance of identified Gram-negative bacteria.
31790895	4	65	theme	intestinal	872:881	arg1	barrier					883:889	intestinal barrier	872:889	intestinal barrier	872:889	METHODS Effects of QHD on gut microbioa and intestinal barrier were evaluated in NASH induced by HFD in mice.
31790895	5	66	theme	rRNA	942:945	arg1	sequencing					947:956	16S rRNA sequencing	938:956	16S rRNA sequencing	938:956	16S rRNA sequencing is employed to analyse the gut microbiota composition.
31790895	1	67	from	participant	225:235	arg1	pathogenesis					244:255	the pathogenesis	240:255	the pathogenesis of non-alcoholic fatty liver disease (NAFLD)	240:300	BACKGROUND Gut microbiota is increasingly recognized as the key participant in the pathogenesis of non-alcoholic fatty liver disease (NAFLD) by translocation of its products, such as lipopolysaccharide (LPS), via the dysfunctional intestinal barrier.
31790895	8	68	theme	colonic	1642:1648	arg1	regulation					1665:1674	the colonic tight junction regulation	1638:1674	the colonic tight junction regulation by QHD	1638:1681	The validated data of phosphorylated proteins suggested that mitogen-activated protein kinase (MAPK) pathway is predominantly responsible for the colonic tight junction regulation by QHD.
31790895	5	69	theme	16S	938:940	arg1	sequencing					947:956	16S rRNA sequencing	938:956	16S rRNA sequencing	938:956	16S rRNA sequencing is employed to analyse the gut microbiota composition.
31790895	6	70	theme	junction	1080:1087	arg1	regulation					1089:1098	tight junction regulation	1074:1098	tight junction regulation	1074:1098	To identify the potential signalling pathway responsible for tight junction regulation, the colonic phosphoprotein profile is screened via the Phospho Explorer Antibody Array and verified in NASH, intestinal barrier dysfunctional mouse and Caco-2 cells.
31790895	2	71	from	clinic	518:523	arg1	China					528:532	China	528:532	China	528:532	Qushi Huayu decoction (QHD), a traditional Chinese medicine, is developed specially for NAFLD and used in clinic in China for more than a decade and previously found to ameliorate non-alcoholic steatohepatitis (NASH) induced by high-fat diet (HFD) in mice accompanied with inhibited metabolic endotoxemia and hepatic LPS signalling.
31790895	9	72	theme	pathway	1792:1798	arg1	downregulation					1758:1771	downregulation	1758:1771	downregulation of intestinal MAPK pathway	1758:1798	CONCLUSION QHD inhibits LPS gut-leakage in NASH, which is associated with downregulation of intestinal MAPK pathway.
31790895	8	73	theme	validated	1500:1508	arg1	data					1510:1513	The validated data	1496:1513	The validated data of phosphorylated proteins	1496:1540	The validated data of phosphorylated proteins suggested that mitogen-activated protein kinase (MAPK) pathway is predominantly responsible for the colonic tight junction regulation by QHD.
31790895	6	74	theme	Antibody	1173:1180	arg1	Array					1182:1186	the Phospho Explorer Antibody Array	1152:1186	the Phospho Explorer Antibody Array	1152:1186	To identify the potential signalling pathway responsible for tight junction regulation, the colonic phosphoprotein profile is screened via the Phospho Explorer Antibody Array and verified in NASH, intestinal barrier dysfunctional mouse and Caco-2 cells.
31790895	2	75	theme	metabolic	695:703	arg1	endotoxemia					705:715	inhibited metabolic endotoxemia	685:715	inhibited metabolic endotoxemia	685:715	Qushi Huayu decoction (QHD), a traditional Chinese medicine, is developed specially for NAFLD and used in clinic in China for more than a decade and previously found to ameliorate non-alcoholic steatohepatitis (NASH) induced by high-fat diet (HFD) in mice accompanied with inhibited metabolic endotoxemia and hepatic LPS signalling.
31790895	1	76	theme	BACKGROUND	161:170	arg1	participant					225:235	the key participant	217:235	the key participant in the pathogenesis of non-alcoholic fatty liver disease (NAFLD)	217:300	BACKGROUND Gut microbiota is increasingly recognized as the key participant in the pathogenesis of non-alcoholic fatty liver disease (NAFLD) by translocation of its products, such as lipopolysaccharide (LPS), via the dysfunctional intestinal barrier.
31790895	1	76	theme	BACKGROUND	161:170	arg1	microbiota					176:185	BACKGROUND Gut microbiota	161:185	BACKGROUND Gut microbiota	161:185	BACKGROUND Gut microbiota is increasingly recognized as the key participant in the pathogenesis of non-alcoholic fatty liver disease (NAFLD) by translocation of its products, such as lipopolysaccharide (LPS), via the dysfunctional intestinal barrier.
31790895	1	77	theme	non-alcoholic	260:272	arg1	disease					286:292	non-alcoholic fatty liver disease	260:292	non-alcoholic fatty liver disease (NAFLD)	260:300	BACKGROUND Gut microbiota is increasingly recognized as the key participant in the pathogenesis of non-alcoholic fatty liver disease (NAFLD) by translocation of its products, such as lipopolysaccharide (LPS), via the dysfunctional intestinal barrier.
31790895	1	77	theme	non-alcoholic	260:272	arg1	NAFLD					295:299	NAFLD	295:299	NAFLD	295:299	BACKGROUND Gut microbiota is increasingly recognized as the key participant in the pathogenesis of non-alcoholic fatty liver disease (NAFLD) by translocation of its products, such as lipopolysaccharide (LPS), via the dysfunctional intestinal barrier.
31790895	8	78	theme	phosphorylated	1518:1531	arg1	proteins					1533:1540	phosphorylated proteins	1518:1540	phosphorylated proteins	1518:1540	The validated data of phosphorylated proteins suggested that mitogen-activated protein kinase (MAPK) pathway is predominantly responsible for the colonic tight junction regulation by QHD.
31790895	6	79	theme	Explorer	1164:1171	arg1	Array					1182:1186	the Phospho Explorer Antibody Array	1152:1186	the Phospho Explorer Antibody Array	1152:1186	To identify the potential signalling pathway responsible for tight junction regulation, the colonic phosphoprotein profile is screened via the Phospho Explorer Antibody Array and verified in NASH, intestinal barrier dysfunctional mouse and Caco-2 cells.
31790895	2	80	theme	inhibited	685:693	arg1	endotoxemia					705:715	inhibited metabolic endotoxemia	685:715	inhibited metabolic endotoxemia	685:715	Qushi Huayu decoction (QHD), a traditional Chinese medicine, is developed specially for NAFLD and used in clinic in China for more than a decade and previously found to ameliorate non-alcoholic steatohepatitis (NASH) induced by high-fat diet (HFD) in mice accompanied with inhibited metabolic endotoxemia and hepatic LPS signalling.
31790895	1	81	theme	Gut	172:174	arg1	participant					225:235	the key participant	217:235	the key participant in the pathogenesis of non-alcoholic fatty liver disease (NAFLD)	217:300	BACKGROUND Gut microbiota is increasingly recognized as the key participant in the pathogenesis of non-alcoholic fatty liver disease (NAFLD) by translocation of its products, such as lipopolysaccharide (LPS), via the dysfunctional intestinal barrier.
31790895	1	81	theme	Gut	172:174	arg1	microbiota					176:185	BACKGROUND Gut microbiota	161:185	BACKGROUND Gut microbiota	161:185	BACKGROUND Gut microbiota is increasingly recognized as the key participant in the pathogenesis of non-alcoholic fatty liver disease (NAFLD) by translocation of its products, such as lipopolysaccharide (LPS), via the dysfunctional intestinal barrier.
31790895	1	82	theme	fatty	274:278	arg1	disease					286:292	non-alcoholic fatty liver disease	260:292	non-alcoholic fatty liver disease (NAFLD)	260:300	BACKGROUND Gut microbiota is increasingly recognized as the key participant in the pathogenesis of non-alcoholic fatty liver disease (NAFLD) by translocation of its products, such as lipopolysaccharide (LPS), via the dysfunctional intestinal barrier.
31790895	1	82	theme	fatty	274:278	arg1	NAFLD					295:299	NAFLD	295:299	NAFLD	295:299	BACKGROUND Gut microbiota is increasingly recognized as the key participant in the pathogenesis of non-alcoholic fatty liver disease (NAFLD) by translocation of its products, such as lipopolysaccharide (LPS), via the dysfunctional intestinal barrier.
31693473	3	0	theme	Phylogenetic	329:340	arg1	analysis					342:349	Phylogenetic analysis	329:349	Phylogenetic analysis based on 16S rRNA gene sequences	329:382	Phylogenetic analysis based on 16S rRNA gene sequences exhibited that strain OTB305T was closely related to Streptomyces bohaiensis JCM 19630T (98.8 %) and Streptomyces lonarensis DSM 42084T (98.8 %).
31693473	4	1	theme	fatty	1009:1013	arg1	iso-C16 					1026:1033	iso-C16 	1026:1033	iso-C16 	1026:1033	The chemotaxonomic characteristics of strain OTB305T corresponded to those of the genus Streptomyces as follows: the diamino acid of the cell-wall peptidoglycan was ll-diaminopimelic acid; whole-cell hydrolysates contained glucose and lacked characteristic major sugars; the predominant isoprenoid quinones were MK-9(H8) and MK-9(H6); the polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and an unidentified phospholipid; the major cellular fatty acids were iso-C16 : 0, C16 : 0 and C16 : 1 ω7c; and the genomic DNA G+C content was 72.83 mol%.
31693473	4	1	theme	fatty	1009:1013	arg1	acids					1015:1019	the major cellular fatty acids	990:1019	the major cellular fatty acids	990:1019	The chemotaxonomic characteristics of strain OTB305T corresponded to those of the genus Streptomyces as follows: the diamino acid of the cell-wall peptidoglycan was ll-diaminopimelic acid; whole-cell hydrolysates contained glucose and lacked characteristic major sugars; the predominant isoprenoid quinones were MK-9(H8) and MK-9(H6); the polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and an unidentified phospholipid; the major cellular fatty acids were iso-C16 : 0, C16 : 0 and C16 : 1 ω7c; and the genomic DNA G+C content was 72.83 mol%.
31693473	5	2	theme	strain	1241:1246	arg1	OTB305T					1248:1254	strain OTB305T	1241:1254	strain OTB305T	1241:1254	However, genomic relatedness analysis based on the average nucleotide identity and some phenotypic characteristics revealed that strain OTB305T was distinguished from closely related Streptomyces species.
31693473	5	3	theme	average	1163:1169	arg1	identity					1182:1189	the average nucleotide identity	1159:1189	the average nucleotide identity	1159:1189	However, genomic relatedness analysis based on the average nucleotide identity and some phenotypic characteristics revealed that strain OTB305T was distinguished from closely related Streptomyces species.
31693473	4	4	theme	cellular	1000:1007	arg1	iso-C16 					1026:1033	iso-C16 	1026:1033	iso-C16 	1026:1033	The chemotaxonomic characteristics of strain OTB305T corresponded to those of the genus Streptomyces as follows: the diamino acid of the cell-wall peptidoglycan was ll-diaminopimelic acid; whole-cell hydrolysates contained glucose and lacked characteristic major sugars; the predominant isoprenoid quinones were MK-9(H8) and MK-9(H6); the polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and an unidentified phospholipid; the major cellular fatty acids were iso-C16 : 0, C16 : 0 and C16 : 1 ω7c; and the genomic DNA G+C content was 72.83 mol%.
31693473	4	4	theme	cellular	1000:1007	arg1	acids					1015:1019	the major cellular fatty acids	990:1019	the major cellular fatty acids	990:1019	The chemotaxonomic characteristics of strain OTB305T corresponded to those of the genus Streptomyces as follows: the diamino acid of the cell-wall peptidoglycan was ll-diaminopimelic acid; whole-cell hydrolysates contained glucose and lacked characteristic major sugars; the predominant isoprenoid quinones were MK-9(H8) and MK-9(H6); the polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and an unidentified phospholipid; the major cellular fatty acids were iso-C16 : 0, C16 : 0 and C16 : 1 ω7c; and the genomic DNA G+C content was 72.83 mol%.
31693473	2	5	theme	producing	133:141	arg1	biosurfactant					143:155	A novel actinobacterium producing biosurfactant	109:155	A novel actinobacterium producing biosurfactant	109:155	A novel actinobacterium producing biosurfactant, designated OTB305T, was isolated from marine sediment sampled at Otsuchi Bay, Iwate Prefecture, Japan and its taxonomic position was examined using a polyphasic approach.
31693473	6	6	theme	novel	1356:1360	arg1	species					1362:1368	a novel species	1354:1368	a novel species	1354:1368	Therefore, strain OTB305T represents a novel species of the genus Streptomyces, for which the name Streptomyces otsuchiensis sp.
31693473	4	7	theme	ll-diaminopimelic	695:711	arg1	acid					713:716	ll-diaminopimelic acid	695:716	ll-diaminopimelic acid	695:716	The chemotaxonomic characteristics of strain OTB305T corresponded to those of the genus Streptomyces as follows: the diamino acid of the cell-wall peptidoglycan was ll-diaminopimelic acid; whole-cell hydrolysates contained glucose and lacked characteristic major sugars; the predominant isoprenoid quinones were MK-9(H8) and MK-9(H6); the polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and an unidentified phospholipid; the major cellular fatty acids were iso-C16 : 0, C16 : 0 and C16 : 1 ω7c; and the genomic DNA G+C content was 72.83 mol%.
31693473	4	7	theme	ll-diaminopimelic	695:711	arg1	acid					655:658	the diamino acid	643:658	the diamino acid of the cell-wall peptidoglycan	643:689	The chemotaxonomic characteristics of strain OTB305T corresponded to those of the genus Streptomyces as follows: the diamino acid of the cell-wall peptidoglycan was ll-diaminopimelic acid; whole-cell hydrolysates contained glucose and lacked characteristic major sugars; the predominant isoprenoid quinones were MK-9(H8) and MK-9(H6); the polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and an unidentified phospholipid; the major cellular fatty acids were iso-C16 : 0, C16 : 0 and C16 : 1 ω7c; and the genomic DNA G+C content was 72.83 mol%.
31693473	5	8	theme	nucleotide	1171:1180	arg1	identity					1182:1189	the average nucleotide identity	1159:1189	the average nucleotide identity	1159:1189	However, genomic relatedness analysis based on the average nucleotide identity and some phenotypic characteristics revealed that strain OTB305T was distinguished from closely related Streptomyces species.
31693473	3	9	theme	gene	369:372	arg1	sequences					374:382	16S rRNA gene sequences	360:382	16S rRNA gene sequences	360:382	Phylogenetic analysis based on 16S rRNA gene sequences exhibited that strain OTB305T was closely related to Streptomyces bohaiensis JCM 19630T (98.8 %) and Streptomyces lonarensis DSM 42084T (98.8 %).
31693473	2	10	theme	actinobacterium	117:131	arg1	biosurfactant					143:155	A novel actinobacterium producing biosurfactant	109:155	A novel actinobacterium producing biosurfactant	109:155	A novel actinobacterium producing biosurfactant, designated OTB305T, was isolated from marine sediment sampled at Otsuchi Bay, Iwate Prefecture, Japan and its taxonomic position was examined using a polyphasic approach.
31693473	4	11	theme	polar	869:873	arg1	phosphatidylglycerol					887:906	phosphatidylglycerol	887:906	phosphatidylglycerol	887:906	The chemotaxonomic characteristics of strain OTB305T corresponded to those of the genus Streptomyces as follows: the diamino acid of the cell-wall peptidoglycan was ll-diaminopimelic acid; whole-cell hydrolysates contained glucose and lacked characteristic major sugars; the predominant isoprenoid quinones were MK-9(H8) and MK-9(H6); the polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and an unidentified phospholipid; the major cellular fatty acids were iso-C16 : 0, C16 : 0 and C16 : 1 ω7c; and the genomic DNA G+C content was 72.83 mol%.
31693473	4	11	theme	polar	869:873	arg1	lipids					875:880	the polar lipids	865:880	the polar lipids	865:880	The chemotaxonomic characteristics of strain OTB305T corresponded to those of the genus Streptomyces as follows: the diamino acid of the cell-wall peptidoglycan was ll-diaminopimelic acid; whole-cell hydrolysates contained glucose and lacked characteristic major sugars; the predominant isoprenoid quinones were MK-9(H8) and MK-9(H6); the polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and an unidentified phospholipid; the major cellular fatty acids were iso-C16 : 0, C16 : 0 and C16 : 1 ω7c; and the genomic DNA G+C content was 72.83 mol%.
31693473	4	12	theme	major	787:791	arg1	sugars					793:798	characteristic major sugars	772:798	characteristic major sugars	772:798	The chemotaxonomic characteristics of strain OTB305T corresponded to those of the genus Streptomyces as follows: the diamino acid of the cell-wall peptidoglycan was ll-diaminopimelic acid; whole-cell hydrolysates contained glucose and lacked characteristic major sugars; the predominant isoprenoid quinones were MK-9(H8) and MK-9(H6); the polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and an unidentified phospholipid; the major cellular fatty acids were iso-C16 : 0, C16 : 0 and C16 : 1 ω7c; and the genomic DNA G+C content was 72.83 mol%.
31693473	3	13	dep	Streptomyces	485:496	arg1	lonarensis					498:507	lonarensis	498:507	lonarensis	498:507	Phylogenetic analysis based on 16S rRNA gene sequences exhibited that strain OTB305T was closely related to Streptomyces bohaiensis JCM 19630T (98.8 %) and Streptomyces lonarensis DSM 42084T (98.8 %).
31693473	4	14	theme	72.83 mol	1100:1108	arg1	%					1109:1109	72.83 mol%	1100:1109	72.83 mol%	1100:1109	The chemotaxonomic characteristics of strain OTB305T corresponded to those of the genus Streptomyces as follows: the diamino acid of the cell-wall peptidoglycan was ll-diaminopimelic acid; whole-cell hydrolysates contained glucose and lacked characteristic major sugars; the predominant isoprenoid quinones were MK-9(H8) and MK-9(H6); the polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and an unidentified phospholipid; the major cellular fatty acids were iso-C16 : 0, C16 : 0 and C16 : 1 ω7c; and the genomic DNA G+C content was 72.83 mol%.
31693473	4	15	theme	diamino	647:653	arg1	acid					713:716	ll-diaminopimelic acid	695:716	ll-diaminopimelic acid	695:716	The chemotaxonomic characteristics of strain OTB305T corresponded to those of the genus Streptomyces as follows: the diamino acid of the cell-wall peptidoglycan was ll-diaminopimelic acid; whole-cell hydrolysates contained glucose and lacked characteristic major sugars; the predominant isoprenoid quinones were MK-9(H8) and MK-9(H6); the polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and an unidentified phospholipid; the major cellular fatty acids were iso-C16 : 0, C16 : 0 and C16 : 1 ω7c; and the genomic DNA G+C content was 72.83 mol%.
31693473	4	15	theme	diamino	647:653	arg1	acid					655:658	the diamino acid	643:658	the diamino acid of the cell-wall peptidoglycan	643:689	The chemotaxonomic characteristics of strain OTB305T corresponded to those of the genus Streptomyces as follows: the diamino acid of the cell-wall peptidoglycan was ll-diaminopimelic acid; whole-cell hydrolysates contained glucose and lacked characteristic major sugars; the predominant isoprenoid quinones were MK-9(H8) and MK-9(H6); the polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and an unidentified phospholipid; the major cellular fatty acids were iso-C16 : 0, C16 : 0 and C16 : 1 ω7c; and the genomic DNA G+C content was 72.83 mol%.
31693473	3	16	theme	16S	360:362	arg1	sequences					374:382	16S rRNA gene sequences	360:382	16S rRNA gene sequences	360:382	Phylogenetic analysis based on 16S rRNA gene sequences exhibited that strain OTB305T was closely related to Streptomyces bohaiensis JCM 19630T (98.8 %) and Streptomyces lonarensis DSM 42084T (98.8 %).
31693473	4	17	contain	contained	743:751	arg2	glucose					753:759	glucose	753:759	glucose	753:759	The chemotaxonomic characteristics of strain OTB305T corresponded to those of the genus Streptomyces as follows: the diamino acid of the cell-wall peptidoglycan was ll-diaminopimelic acid; whole-cell hydrolysates contained glucose and lacked characteristic major sugars; the predominant isoprenoid quinones were MK-9(H8) and MK-9(H6); the polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and an unidentified phospholipid; the major cellular fatty acids were iso-C16 : 0, C16 : 0 and C16 : 1 ω7c; and the genomic DNA G+C content was 72.83 mol%.
31693473	4	17	contain	contained	743:751	arg1	hydrolysates					730:741	whole-cell hydrolysates	719:741	whole-cell hydrolysates	719:741	The chemotaxonomic characteristics of strain OTB305T corresponded to those of the genus Streptomyces as follows: the diamino acid of the cell-wall peptidoglycan was ll-diaminopimelic acid; whole-cell hydrolysates contained glucose and lacked characteristic major sugars; the predominant isoprenoid quinones were MK-9(H8) and MK-9(H6); the polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and an unidentified phospholipid; the major cellular fatty acids were iso-C16 : 0, C16 : 0 and C16 : 1 ω7c; and the genomic DNA G+C content was 72.83 mol%.
31693473	4	18	theme	cell-wall	667:675	arg1	peptidoglycan					677:689	the cell-wall peptidoglycan	663:689	the cell-wall peptidoglycan	663:689	The chemotaxonomic characteristics of strain OTB305T corresponded to those of the genus Streptomyces as follows: the diamino acid of the cell-wall peptidoglycan was ll-diaminopimelic acid; whole-cell hydrolysates contained glucose and lacked characteristic major sugars; the predominant isoprenoid quinones were MK-9(H8) and MK-9(H6); the polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and an unidentified phospholipid; the major cellular fatty acids were iso-C16 : 0, C16 : 0 and C16 : 1 ω7c; and the genomic DNA G+C content was 72.83 mol%.
31693473	3	19	theme	JCM	461:463	arg1	19630T					465:470	Streptomyces bohaiensis JCM 19630T	437:470	Streptomyces bohaiensis JCM 19630T (98.8 %)	437:479	Phylogenetic analysis based on 16S rRNA gene sequences exhibited that strain OTB305T was closely related to Streptomyces bohaiensis JCM 19630T (98.8 %) and Streptomyces lonarensis DSM 42084T (98.8 %).
31693473	3	19	theme	JCM	461:463	arg1	%					478:478	98.8 %	473:478	98.8 %	473:478	Phylogenetic analysis based on 16S rRNA gene sequences exhibited that strain OTB305T was closely related to Streptomyces bohaiensis JCM 19630T (98.8 %) and Streptomyces lonarensis DSM 42084T (98.8 %).
31693473	3	20	theme	rRNA	364:367	arg1	sequences					374:382	16S rRNA gene sequences	360:382	16S rRNA gene sequences	360:382	Phylogenetic analysis based on 16S rRNA gene sequences exhibited that strain OTB305T was closely related to Streptomyces bohaiensis JCM 19630T (98.8 %) and Streptomyces lonarensis DSM 42084T (98.8 %).
31693473	1	21	theme	marine	92:97	arg1	sediment					99:106	marine sediment	92:106	marine sediment	92:106	nov., a biosurfactant-producing actinobacterium isolated from marine sediment.
31693473	5	22	theme	genomic	1121:1127	arg1	analysis					1141:1148	genomic relatedness analysis	1121:1148	genomic relatedness analysis based on the average nucleotide identity and some phenotypic characteristics	1121:1225	However, genomic relatedness analysis based on the average nucleotide identity and some phenotypic characteristics revealed that strain OTB305T was distinguished from closely related Streptomyces species.
31693473	0	23	theme	otsuchiensis	13:24	arg1	sp					26:27	Streptomyces otsuchiensis sp	0:27	Streptomyces otsuchiensis sp.	0:28	Streptomyces otsuchiensis sp.
31693473	3	24	theme	Streptomyces	437:448	arg1	19630T					465:470	Streptomyces bohaiensis JCM 19630T	437:470	Streptomyces bohaiensis JCM 19630T (98.8 %)	437:479	Phylogenetic analysis based on 16S rRNA gene sequences exhibited that strain OTB305T was closely related to Streptomyces bohaiensis JCM 19630T (98.8 %) and Streptomyces lonarensis DSM 42084T (98.8 %).
31693473	3	24	theme	Streptomyces	437:448	arg1	%					478:478	98.8 %	473:478	98.8 %	473:478	Phylogenetic analysis based on 16S rRNA gene sequences exhibited that strain OTB305T was closely related to Streptomyces bohaiensis JCM 19630T (98.8 %) and Streptomyces lonarensis DSM 42084T (98.8 %).
31693473	0	25	theme	Streptomyces	0:11	arg1	sp					26:27	Streptomyces otsuchiensis sp	0:27	Streptomyces otsuchiensis sp.	0:28	Streptomyces otsuchiensis sp.
31693473	4	26	theme	genomic	1072:1078	arg1	G+C content					1084:1094	the genomic DNA G+C content	1068:1094	the genomic DNA G+C content	1068:1094	The chemotaxonomic characteristics of strain OTB305T corresponded to those of the genus Streptomyces as follows: the diamino acid of the cell-wall peptidoglycan was ll-diaminopimelic acid; whole-cell hydrolysates contained glucose and lacked characteristic major sugars; the predominant isoprenoid quinones were MK-9(H8) and MK-9(H6); the polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and an unidentified phospholipid; the major cellular fatty acids were iso-C16 : 0, C16 : 0 and C16 : 1 ω7c; and the genomic DNA G+C content was 72.83 mol%.
31693473	6	27	theme	otsuchiensis	1429:1440	arg1	sp					1442:1443	the name Streptomyces otsuchiensis sp	1407:1443	the name Streptomyces otsuchiensis sp	1407:1443	Therefore, strain OTB305T represents a novel species of the genus Streptomyces, for which the name Streptomyces otsuchiensis sp.
31693473	6	28	theme	genus	1377:1381	arg1	Streptomyces					1383:1394	the genus Streptomyces	1373:1394	the genus Streptomyces	1373:1394	Therefore, strain OTB305T represents a novel species of the genus Streptomyces, for which the name Streptomyces otsuchiensis sp.
31693473	2	29	theme	polyphasic	308:317	arg1	approach					319:326	a polyphasic approach	306:326	a polyphasic approach	306:326	A novel actinobacterium producing biosurfactant, designated OTB305T, was isolated from marine sediment sampled at Otsuchi Bay, Iwate Prefecture, Japan and its taxonomic position was examined using a polyphasic approach.
31693473	6	30	theme	Streptomyces	1416:1427	arg1	sp					1442:1443	the name Streptomyces otsuchiensis sp	1407:1443	the name Streptomyces otsuchiensis sp	1407:1443	Therefore, strain OTB305T represents a novel species of the genus Streptomyces, for which the name Streptomyces otsuchiensis sp.
31693473	4	31	theme	OTB305T	575:581	arg1	characteristics					549:563	The chemotaxonomic characteristics	530:563	The chemotaxonomic characteristics of strain OTB305T	530:581	The chemotaxonomic characteristics of strain OTB305T corresponded to those of the genus Streptomyces as follows: the diamino acid of the cell-wall peptidoglycan was ll-diaminopimelic acid; whole-cell hydrolysates contained glucose and lacked characteristic major sugars; the predominant isoprenoid quinones were MK-9(H8) and MK-9(H6); the polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and an unidentified phospholipid; the major cellular fatty acids were iso-C16 : 0, C16 : 0 and C16 : 1 ω7c; and the genomic DNA G+C content was 72.83 mol%.
31693473	5	32	theme	Streptomyces	1295:1306	arg1	species					1308:1314	closely related Streptomyces species	1279:1314	closely related Streptomyces species	1279:1314	However, genomic relatedness analysis based on the average nucleotide identity and some phenotypic characteristics revealed that strain OTB305T was distinguished from closely related Streptomyces species.
31693473	6	33	theme	name	1411:1414	arg1	sp					1442:1443	the name Streptomyces otsuchiensis sp	1407:1443	the name Streptomyces otsuchiensis sp	1407:1443	Therefore, strain OTB305T represents a novel species of the genus Streptomyces, for which the name Streptomyces otsuchiensis sp.
31693473	4	34	theme	strain	568:573	arg1	OTB305T					575:581	strain OTB305T	568:581	strain OTB305T	568:581	The chemotaxonomic characteristics of strain OTB305T corresponded to those of the genus Streptomyces as follows: the diamino acid of the cell-wall peptidoglycan was ll-diaminopimelic acid; whole-cell hydrolysates contained glucose and lacked characteristic major sugars; the predominant isoprenoid quinones were MK-9(H8) and MK-9(H6); the polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and an unidentified phospholipid; the major cellular fatty acids were iso-C16 : 0, C16 : 0 and C16 : 1 ω7c; and the genomic DNA G+C content was 72.83 mol%.
31693473	6	35	theme	strain	1328:1333	arg1	OTB305T					1335:1341	strain OTB305T	1328:1341	strain OTB305T	1328:1341	Therefore, strain OTB305T represents a novel species of the genus Streptomyces, for which the name Streptomyces otsuchiensis sp.
31693473	2	36	theme	marine	196:201	arg1	sediment					203:210	marine sediment	196:210	marine sediment sampled at Otsuchi Bay, Iwate Prefecture, Japan	196:258	A novel actinobacterium producing biosurfactant, designated OTB305T, was isolated from marine sediment sampled at Otsuchi Bay, Iwate Prefecture, Japan and its taxonomic position was examined using a polyphasic approach.
31693473	3	37	dep	Streptomyces	437:448	arg1	bohaiensis					450:459	bohaiensis	450:459	bohaiensis	450:459	Phylogenetic analysis based on 16S rRNA gene sequences exhibited that strain OTB305T was closely related to Streptomyces bohaiensis JCM 19630T (98.8 %) and Streptomyces lonarensis DSM 42084T (98.8 %).
31693473	4	38	theme	DNA	1080:1082	arg1	G+C content					1084:1094	the genomic DNA G+C content	1068:1094	the genomic DNA G+C content	1068:1094	The chemotaxonomic characteristics of strain OTB305T corresponded to those of the genus Streptomyces as follows: the diamino acid of the cell-wall peptidoglycan was ll-diaminopimelic acid; whole-cell hydrolysates contained glucose and lacked characteristic major sugars; the predominant isoprenoid quinones were MK-9(H8) and MK-9(H6); the polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and an unidentified phospholipid; the major cellular fatty acids were iso-C16 : 0, C16 : 0 and C16 : 1 ω7c; and the genomic DNA G+C content was 72.83 mol%.
31693473	2	39	attach	isolated	182:189	arg1	sediment					203:210	marine sediment	196:210	marine sediment sampled at Otsuchi Bay, Iwate Prefecture, Japan	196:258	A novel actinobacterium producing biosurfactant, designated OTB305T, was isolated from marine sediment sampled at Otsuchi Bay, Iwate Prefecture, Japan and its taxonomic position was examined using a polyphasic approach.
31693473	2	39	attach	isolated	182:189	arg2	biosurfactant					143:155	A novel actinobacterium producing biosurfactant	109:155	A novel actinobacterium producing biosurfactant	109:155	A novel actinobacterium producing biosurfactant, designated OTB305T, was isolated from marine sediment sampled at Otsuchi Bay, Iwate Prefecture, Japan and its taxonomic position was examined using a polyphasic approach.
31693473	2	40	theme	novel	111:115	arg1	biosurfactant					143:155	A novel actinobacterium producing biosurfactant	109:155	A novel actinobacterium producing biosurfactant	109:155	A novel actinobacterium producing biosurfactant, designated OTB305T, was isolated from marine sediment sampled at Otsuchi Bay, Iwate Prefecture, Japan and its taxonomic position was examined using a polyphasic approach.
31693473	4	41	theme	characteristic	772:785	arg1	sugars					793:798	characteristic major sugars	772:798	characteristic major sugars	772:798	The chemotaxonomic characteristics of strain OTB305T corresponded to those of the genus Streptomyces as follows: the diamino acid of the cell-wall peptidoglycan was ll-diaminopimelic acid; whole-cell hydrolysates contained glucose and lacked characteristic major sugars; the predominant isoprenoid quinones were MK-9(H8) and MK-9(H6); the polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and an unidentified phospholipid; the major cellular fatty acids were iso-C16 : 0, C16 : 0 and C16 : 1 ω7c; and the genomic DNA G+C content was 72.83 mol%.
31693473	4	42	theme	unidentified	963:974	arg1	phospholipid					976:987	an unidentified phospholipid	960:987	an unidentified phospholipid	960:987	The chemotaxonomic characteristics of strain OTB305T corresponded to those of the genus Streptomyces as follows: the diamino acid of the cell-wall peptidoglycan was ll-diaminopimelic acid; whole-cell hydrolysates contained glucose and lacked characteristic major sugars; the predominant isoprenoid quinones were MK-9(H8) and MK-9(H6); the polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and an unidentified phospholipid; the major cellular fatty acids were iso-C16 : 0, C16 : 0 and C16 : 1 ω7c; and the genomic DNA G+C content was 72.83 mol%.
31693473	4	43	theme	whole-cell	719:728	arg1	hydrolysates					730:741	whole-cell hydrolysates	719:741	whole-cell hydrolysates	719:741	The chemotaxonomic characteristics of strain OTB305T corresponded to those of the genus Streptomyces as follows: the diamino acid of the cell-wall peptidoglycan was ll-diaminopimelic acid; whole-cell hydrolysates contained glucose and lacked characteristic major sugars; the predominant isoprenoid quinones were MK-9(H8) and MK-9(H6); the polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and an unidentified phospholipid; the major cellular fatty acids were iso-C16 : 0, C16 : 0 and C16 : 1 ω7c; and the genomic DNA G+C content was 72.83 mol%.
31693473	8	44	theme	=NBRC	1492:1496	arg1	OTB305T					1483:1489	OTB305T	1483:1489	OTB305T (=NBRC 113255T=TBRC 9682T)	1483:1516	The type strain is OTB305T (=NBRC 113255T=TBRC 9682T).
31693473	8	44	theme	=NBRC	1492:1496	arg1	9682T					1511:1515	=NBRC 113255T=TBRC 9682T	1492:1515	=NBRC 113255T=TBRC 9682T	1492:1515	The type strain is OTB305T (=NBRC 113255T=TBRC 9682T).
31693473	4	45	theme	chemotaxonomic	534:547	arg1	characteristics					549:563	The chemotaxonomic characteristics	530:563	The chemotaxonomic characteristics of strain OTB305T	530:581	The chemotaxonomic characteristics of strain OTB305T corresponded to those of the genus Streptomyces as follows: the diamino acid of the cell-wall peptidoglycan was ll-diaminopimelic acid; whole-cell hydrolysates contained glucose and lacked characteristic major sugars; the predominant isoprenoid quinones were MK-9(H8) and MK-9(H6); the polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and an unidentified phospholipid; the major cellular fatty acids were iso-C16 : 0, C16 : 0 and C16 : 1 ω7c; and the genomic DNA G+C content was 72.83 mol%.
31693473	8	46	theme	type	1468:1471	arg1	OTB305T					1483:1489	OTB305T	1483:1489	OTB305T (=NBRC 113255T=TBRC 9682T)	1483:1516	The type strain is OTB305T (=NBRC 113255T=TBRC 9682T).
31693473	8	46	theme	type	1468:1471	arg1	strain					1473:1478	The type strain	1464:1478	The type strain	1464:1478	The type strain is OTB305T (=NBRC 113255T=TBRC 9682T).
31693473	3	47	theme	DSM	509:511	arg1	42084T					513:518	Streptomyces lonarensis DSM 42084T	485:518	Streptomyces lonarensis DSM 42084T (98.8 %)	485:527	Phylogenetic analysis based on 16S rRNA gene sequences exhibited that strain OTB305T was closely related to Streptomyces bohaiensis JCM 19630T (98.8 %) and Streptomyces lonarensis DSM 42084T (98.8 %).
31693473	3	47	theme	DSM	509:511	arg1	%					526:526	98.8 %	521:526	98.8 %	521:526	Phylogenetic analysis based on 16S rRNA gene sequences exhibited that strain OTB305T was closely related to Streptomyces bohaiensis JCM 19630T (98.8 %) and Streptomyces lonarensis DSM 42084T (98.8 %).
31693473	5	48	theme	relatedness	1129:1139	arg1	analysis					1141:1148	genomic relatedness analysis	1121:1148	genomic relatedness analysis based on the average nucleotide identity and some phenotypic characteristics	1121:1225	However, genomic relatedness analysis based on the average nucleotide identity and some phenotypic characteristics revealed that strain OTB305T was distinguished from closely related Streptomyces species.
31693473	4	49	theme	isoprenoid	817:826	arg1	quinones					828:835	the predominant isoprenoid quinones	801:835	the predominant isoprenoid quinones	801:835	The chemotaxonomic characteristics of strain OTB305T corresponded to those of the genus Streptomyces as follows: the diamino acid of the cell-wall peptidoglycan was ll-diaminopimelic acid; whole-cell hydrolysates contained glucose and lacked characteristic major sugars; the predominant isoprenoid quinones were MK-9(H8) and MK-9(H6); the polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and an unidentified phospholipid; the major cellular fatty acids were iso-C16 : 0, C16 : 0 and C16 : 1 ω7c; and the genomic DNA G+C content was 72.83 mol%.
31693473	4	49	theme	isoprenoid	817:826	arg1	MK-9					842:845	MK-9	842:845	MK-9	842:845	The chemotaxonomic characteristics of strain OTB305T corresponded to those of the genus Streptomyces as follows: the diamino acid of the cell-wall peptidoglycan was ll-diaminopimelic acid; whole-cell hydrolysates contained glucose and lacked characteristic major sugars; the predominant isoprenoid quinones were MK-9(H8) and MK-9(H6); the polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and an unidentified phospholipid; the major cellular fatty acids were iso-C16 : 0, C16 : 0 and C16 : 1 ω7c; and the genomic DNA G+C content was 72.83 mol%.
31693473	2	50	theme	taxonomic	268:276	arg1	position					278:285	its taxonomic position	264:285	its taxonomic position	264:285	A novel actinobacterium producing biosurfactant, designated OTB305T, was isolated from marine sediment sampled at Otsuchi Bay, Iwate Prefecture, Japan and its taxonomic position was examined using a polyphasic approach.
31693473	4	51	theme	peptidoglycan	677:689	arg1	acid					713:716	ll-diaminopimelic acid	695:716	ll-diaminopimelic acid	695:716	The chemotaxonomic characteristics of strain OTB305T corresponded to those of the genus Streptomyces as follows: the diamino acid of the cell-wall peptidoglycan was ll-diaminopimelic acid; whole-cell hydrolysates contained glucose and lacked characteristic major sugars; the predominant isoprenoid quinones were MK-9(H8) and MK-9(H6); the polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and an unidentified phospholipid; the major cellular fatty acids were iso-C16 : 0, C16 : 0 and C16 : 1 ω7c; and the genomic DNA G+C content was 72.83 mol%.
31693473	4	51	theme	peptidoglycan	677:689	arg1	acid					655:658	the diamino acid	643:658	the diamino acid of the cell-wall peptidoglycan	643:689	The chemotaxonomic characteristics of strain OTB305T corresponded to those of the genus Streptomyces as follows: the diamino acid of the cell-wall peptidoglycan was ll-diaminopimelic acid; whole-cell hydrolysates contained glucose and lacked characteristic major sugars; the predominant isoprenoid quinones were MK-9(H8) and MK-9(H6); the polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and an unidentified phospholipid; the major cellular fatty acids were iso-C16 : 0, C16 : 0 and C16 : 1 ω7c; and the genomic DNA G+C content was 72.83 mol%.
31693473	4	52	theme	 1	1056:1057	arg1	ω7c					1059:1061	 1 ω7c	1056:1061	 1 ω7c	1056:1061	The chemotaxonomic characteristics of strain OTB305T corresponded to those of the genus Streptomyces as follows: the diamino acid of the cell-wall peptidoglycan was ll-diaminopimelic acid; whole-cell hydrolysates contained glucose and lacked characteristic major sugars; the predominant isoprenoid quinones were MK-9(H8) and MK-9(H6); the polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and an unidentified phospholipid; the major cellular fatty acids were iso-C16 : 0, C16 : 0 and C16 : 1 ω7c; and the genomic DNA G+C content was 72.83 mol%.
31693473	4	53	theme	genus	612:616	arg1	Streptomyces					618:629	the genus Streptomyces	608:629	the genus Streptomyces	608:629	The chemotaxonomic characteristics of strain OTB305T corresponded to those of the genus Streptomyces as follows: the diamino acid of the cell-wall peptidoglycan was ll-diaminopimelic acid; whole-cell hydrolysates contained glucose and lacked characteristic major sugars; the predominant isoprenoid quinones were MK-9(H8) and MK-9(H6); the polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and an unidentified phospholipid; the major cellular fatty acids were iso-C16 : 0, C16 : 0 and C16 : 1 ω7c; and the genomic DNA G+C content was 72.83 mol%.
31693473	4	54	theme	predominant	805:815	arg1	quinones					828:835	the predominant isoprenoid quinones	801:835	the predominant isoprenoid quinones	801:835	The chemotaxonomic characteristics of strain OTB305T corresponded to those of the genus Streptomyces as follows: the diamino acid of the cell-wall peptidoglycan was ll-diaminopimelic acid; whole-cell hydrolysates contained glucose and lacked characteristic major sugars; the predominant isoprenoid quinones were MK-9(H8) and MK-9(H6); the polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and an unidentified phospholipid; the major cellular fatty acids were iso-C16 : 0, C16 : 0 and C16 : 1 ω7c; and the genomic DNA G+C content was 72.83 mol%.
31693473	4	54	theme	predominant	805:815	arg1	MK-9					842:845	MK-9	842:845	MK-9	842:845	The chemotaxonomic characteristics of strain OTB305T corresponded to those of the genus Streptomyces as follows: the diamino acid of the cell-wall peptidoglycan was ll-diaminopimelic acid; whole-cell hydrolysates contained glucose and lacked characteristic major sugars; the predominant isoprenoid quinones were MK-9(H8) and MK-9(H6); the polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and an unidentified phospholipid; the major cellular fatty acids were iso-C16 : 0, C16 : 0 and C16 : 1 ω7c; and the genomic DNA G+C content was 72.83 mol%.
31693473	3	55	theme	Streptomyces	485:496	arg1	42084T					513:518	Streptomyces lonarensis DSM 42084T	485:518	Streptomyces lonarensis DSM 42084T (98.8 %)	485:527	Phylogenetic analysis based on 16S rRNA gene sequences exhibited that strain OTB305T was closely related to Streptomyces bohaiensis JCM 19630T (98.8 %) and Streptomyces lonarensis DSM 42084T (98.8 %).
31693473	3	55	theme	Streptomyces	485:496	arg1	%					526:526	98.8 %	521:526	98.8 %	521:526	Phylogenetic analysis based on 16S rRNA gene sequences exhibited that strain OTB305T was closely related to Streptomyces bohaiensis JCM 19630T (98.8 %) and Streptomyces lonarensis DSM 42084T (98.8 %).
31693473	5	56	theme	phenotypic	1200:1209	arg1	characteristics					1211:1225	some phenotypic characteristics	1195:1225	some phenotypic characteristics	1195:1225	However, genomic relatedness analysis based on the average nucleotide identity and some phenotypic characteristics revealed that strain OTB305T was distinguished from closely related Streptomyces species.
31693473	4	57	theme	major	994:998	arg1	iso-C16 					1026:1033	iso-C16 	1026:1033	iso-C16 	1026:1033	The chemotaxonomic characteristics of strain OTB305T corresponded to those of the genus Streptomyces as follows: the diamino acid of the cell-wall peptidoglycan was ll-diaminopimelic acid; whole-cell hydrolysates contained glucose and lacked characteristic major sugars; the predominant isoprenoid quinones were MK-9(H8) and MK-9(H6); the polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and an unidentified phospholipid; the major cellular fatty acids were iso-C16 : 0, C16 : 0 and C16 : 1 ω7c; and the genomic DNA G+C content was 72.83 mol%.
31693473	4	57	theme	major	994:998	arg1	acids					1015:1019	the major cellular fatty acids	990:1019	the major cellular fatty acids	990:1019	The chemotaxonomic characteristics of strain OTB305T corresponded to those of the genus Streptomyces as follows: the diamino acid of the cell-wall peptidoglycan was ll-diaminopimelic acid; whole-cell hydrolysates contained glucose and lacked characteristic major sugars; the predominant isoprenoid quinones were MK-9(H8) and MK-9(H6); the polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and an unidentified phospholipid; the major cellular fatty acids were iso-C16 : 0, C16 : 0 and C16 : 1 ω7c; and the genomic DNA G+C content was 72.83 mol%.
31693473	4	58	dep	MK-9	842:845	arg1	phosphatidylinositol					935:954	phosphatidylinositol	935:954	phosphatidylinositol	935:954	The chemotaxonomic characteristics of strain OTB305T corresponded to those of the genus Streptomyces as follows: the diamino acid of the cell-wall peptidoglycan was ll-diaminopimelic acid; whole-cell hydrolysates contained glucose and lacked characteristic major sugars; the predominant isoprenoid quinones were MK-9(H8) and MK-9(H6); the polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and an unidentified phospholipid; the major cellular fatty acids were iso-C16 : 0, C16 : 0 and C16 : 1 ω7c; and the genomic DNA G+C content was 72.83 mol%.
31693473	4	58	dep	MK-9	842:845	arg1	lipids					875:880	the polar lipids	865:880	the polar lipids	865:880	The chemotaxonomic characteristics of strain OTB305T corresponded to those of the genus Streptomyces as follows: the diamino acid of the cell-wall peptidoglycan was ll-diaminopimelic acid; whole-cell hydrolysates contained glucose and lacked characteristic major sugars; the predominant isoprenoid quinones were MK-9(H8) and MK-9(H6); the polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and an unidentified phospholipid; the major cellular fatty acids were iso-C16 : 0, C16 : 0 and C16 : 1 ω7c; and the genomic DNA G+C content was 72.83 mol%.
31693473	4	58	dep	MK-9	842:845	arg1	 0					1035:1036	 0	1035:1036	 0	1035:1036	The chemotaxonomic characteristics of strain OTB305T corresponded to those of the genus Streptomyces as follows: the diamino acid of the cell-wall peptidoglycan was ll-diaminopimelic acid; whole-cell hydrolysates contained glucose and lacked characteristic major sugars; the predominant isoprenoid quinones were MK-9(H8) and MK-9(H6); the polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and an unidentified phospholipid; the major cellular fatty acids were iso-C16 : 0, C16 : 0 and C16 : 1 ω7c; and the genomic DNA G+C content was 72.83 mol%.
31693473	4	58	dep	MK-9	842:845	arg1	phosphatidylglycerol					887:906	phosphatidylglycerol	887:906	phosphatidylglycerol	887:906	The chemotaxonomic characteristics of strain OTB305T corresponded to those of the genus Streptomyces as follows: the diamino acid of the cell-wall peptidoglycan was ll-diaminopimelic acid; whole-cell hydrolysates contained glucose and lacked characteristic major sugars; the predominant isoprenoid quinones were MK-9(H8) and MK-9(H6); the polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and an unidentified phospholipid; the major cellular fatty acids were iso-C16 : 0, C16 : 0 and C16 : 1 ω7c; and the genomic DNA G+C content was 72.83 mol%.
31693473	4	58	dep	MK-9	842:845	arg1	phospholipid					976:987	an unidentified phospholipid	960:987	an unidentified phospholipid	960:987	The chemotaxonomic characteristics of strain OTB305T corresponded to those of the genus Streptomyces as follows: the diamino acid of the cell-wall peptidoglycan was ll-diaminopimelic acid; whole-cell hydrolysates contained glucose and lacked characteristic major sugars; the predominant isoprenoid quinones were MK-9(H8) and MK-9(H6); the polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and an unidentified phospholipid; the major cellular fatty acids were iso-C16 : 0, C16 : 0 and C16 : 1 ω7c; and the genomic DNA G+C content was 72.83 mol%.
31693473	4	58	dep	MK-9	842:845	arg1	phosphatidylethanolamine					909:932	phosphatidylethanolamine	909:932	phosphatidylethanolamine	909:932	The chemotaxonomic characteristics of strain OTB305T corresponded to those of the genus Streptomyces as follows: the diamino acid of the cell-wall peptidoglycan was ll-diaminopimelic acid; whole-cell hydrolysates contained glucose and lacked characteristic major sugars; the predominant isoprenoid quinones were MK-9(H8) and MK-9(H6); the polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and an unidentified phospholipid; the major cellular fatty acids were iso-C16 : 0, C16 : 0 and C16 : 1 ω7c; and the genomic DNA G+C content was 72.83 mol%.
31693473	5	59	theme	related	1287:1293	arg1	species					1308:1314	closely related Streptomyces species	1279:1314	closely related Streptomyces species	1279:1314	However, genomic relatedness analysis based on the average nucleotide identity and some phenotypic characteristics revealed that strain OTB305T was distinguished from closely related Streptomyces species.
31693473	6	60	theme	Streptomyces	1383:1394	arg1	species					1362:1368	a novel species	1354:1368	a novel species	1354:1368	Therefore, strain OTB305T represents a novel species of the genus Streptomyces, for which the name Streptomyces otsuchiensis sp.
31693473	1	61	theme	biosurfactant-producing	38:60	arg1	actinobacterium					62:76	a biosurfactant-producing actinobacterium	36:76	a biosurfactant-producing actinobacterium	36:76	nov., a biosurfactant-producing actinobacterium isolated from marine sediment.
31693473	1	61	theme	biosurfactant-producing	38:60	arg1	nov.					30:33	nov.	30:33	nov.	30:33	nov., a biosurfactant-producing actinobacterium isolated from marine sediment.
31693473	3	62	theme	strain	399:404	arg1	OTB305T					406:412	strain OTB305T	399:412	strain OTB305T	399:412	Phylogenetic analysis based on 16S rRNA gene sequences exhibited that strain OTB305T was closely related to Streptomyces bohaiensis JCM 19630T (98.8 %) and Streptomyces lonarensis DSM 42084T (98.8 %).
31693473	8	63	theme	113255T=TBRC	1498:1509	arg1	OTB305T					1483:1489	OTB305T	1483:1489	OTB305T (=NBRC 113255T=TBRC 9682T)	1483:1516	The type strain is OTB305T (=NBRC 113255T=TBRC 9682T).
31693473	8	63	theme	113255T=TBRC	1498:1509	arg1	9682T					1511:1515	=NBRC 113255T=TBRC 9682T	1492:1515	=NBRC 113255T=TBRC 9682T	1492:1515	The type strain is OTB305T (=NBRC 113255T=TBRC 9682T).
31573843	0	0	theme	oxidation	82:90	arg1	profile					92:98	whole body postprandial oxidation profile	58:98	whole body postprandial oxidation profile under a low-protein diet	58:123	Liver GCN2 controls hepatic FGF21 secretion and modulates whole body postprandial oxidation profile under a low-protein diet.
31573843	6	1	from	GCN2	862:865	arg1	mice					873:876	WT mice	870:876	WT mice	870:876	On the contrary, a low-protein diet activates GCN2 in WT mice, inducing FGF21 secretion.
31573843	5	2	from	pathway	702:708	arg1	liver					717:721	the liver	713:721	the liver	713:721	In mice fed normo- and high-protein diets, GCN2 pathway in the liver is not activated in WT mice, leading to a similar metabolic profile with the one of KO mice.
31573843	3	3	theme	GCN2-deficient	452:465	arg1	mice					472:475	GCN2-deficient (KO) mice	452:475	GCN2-deficient (KO) mice	452:475	Wild-type (WT) and liver GCN2-deficient (KO) mice were fed either a normo-protein diet, a low-protein diet, or a high-protein diet for 3 wk.
31573843	0	4	theme	postprandial	69:80	arg1	profile					92:98	whole body postprandial oxidation profile	58:98	whole body postprandial oxidation profile under a low-protein diet	58:123	Liver GCN2 controls hepatic FGF21 secretion and modulates whole body postprandial oxidation profile under a low-protein diet.
31573843	7	5	theme	lipid	946:950	arg1	oxidation					952:960	lipid oxidation	946:960	lipid oxidation	946:960	In turn, FGF21 maintains a high level of lipid oxidation, leading to a different postprandial oxidation profile compared with KO mice.
31573843	2	6	from	role	303:306	arg1	adaptations					341:351	the metabolic adaptations	327:351	the metabolic adaptations in response to the modulation of dietary protein	327:400	However, the role of hepatic GCN2 in the metabolic adaptations in response to the modulation of dietary protein has been seldom studied.
31573843	2	7	theme	GCN2	319:322	arg1	role					303:306	the role	299:306	the role of hepatic GCN2 in the metabolic adaptations in response to the modulation of dietary protein	299:400	However, the role of hepatic GCN2 in the metabolic adaptations in response to the modulation of dietary protein has been seldom studied.
31573843	6	8	theme	low-protein	835:845	arg1	diet					847:850	a low-protein diet	833:850	a low-protein diet	833:850	On the contrary, a low-protein diet activates GCN2 in WT mice, inducing FGF21 secretion.
31573843	7	9	theme	high	932:935	arg1	level					937:941	a high level	930:941	a high level of lipid oxidation	930:960	In turn, FGF21 maintains a high level of lipid oxidation, leading to a different postprandial oxidation profile compared with KO mice.
31573843	5	10	theme	mice	810:813	arg1	one					800:802	one	800:802	one	800:802	In mice fed normo- and high-protein diets, GCN2 pathway in the liver is not activated in WT mice, leading to a similar metabolic profile with the one of KO mice.
31573843	5	10	theme	mice	810:813	arg1	mice					810:813	KO mice	807:813	KO mice	807:813	In mice fed normo- and high-protein diets, GCN2 pathway in the liver is not activated in WT mice, leading to a similar metabolic profile with the one of KO mice.
31573843	7	11	theme	KO	1031:1032	arg1	mice					1034:1037	KO mice	1031:1037	KO mice	1031:1037	In turn, FGF21 maintains a high level of lipid oxidation, leading to a different postprandial oxidation profile compared with KO mice.
31573843	4	12	theme	food	601:604	arg1	intake					606:611	food intake	601:611	food intake	601:611	During this period, body weight, food intake, and metabolic parameters were followed.
31573843	8	13	theme	whole	1119:1123	arg1	profile					1153:1159	a whole body postprandial oxidation profile	1117:1159	a whole body postprandial oxidation profile	1117:1159	Hepatic GCN2 controls FGF21 secretion under a low-protein diet and modulates a whole body postprandial oxidation profile.
31573843	0	14	theme	low-protein	108:118	arg1	diet					120:123	a low-protein diet	106:123	a low-protein diet	106:123	Liver GCN2 controls hepatic FGF21 secretion and modulates whole body postprandial oxidation profile under a low-protein diet.
31573843	5	15	theme	metabolic	773:781	arg1	profile					783:789	a similar metabolic profile	763:789	a similar metabolic profile with the one of KO mice	763:813	In mice fed normo- and high-protein diets, GCN2 pathway in the liver is not activated in WT mice, leading to a similar metabolic profile with the one of KO mice.
31573843	8	16	theme	low-protein	1086:1096	arg1	diet					1098:1101	a low-protein diet	1084:1101	a low-protein diet	1084:1101	Hepatic GCN2 controls FGF21 secretion under a low-protein diet and modulates a whole body postprandial oxidation profile.
31573843	8	17	theme	FGF21	1062:1066	arg1	secretion					1068:1076	FGF21 secretion	1062:1076	FGF21 secretion	1062:1076	Hepatic GCN2 controls FGF21 secretion under a low-protein diet and modulates a whole body postprandial oxidation profile.
31573843	5	18	theme	similar	765:771	arg1	profile					783:789	a similar metabolic profile	763:789	a similar metabolic profile with the one of KO mice	763:813	In mice fed normo- and high-protein diets, GCN2 pathway in the liver is not activated in WT mice, leading to a similar metabolic profile with the one of KO mice.
31573843	7	19	theme	oxidation	999:1007	arg1	profile					1009:1015	a different postprandial oxidation profile	974:1015	a different postprandial oxidation profile compared with KO mice	974:1037	In turn, FGF21 maintains a high level of lipid oxidation, leading to a different postprandial oxidation profile compared with KO mice.
31573843	5	20	theme	WT	743:744	arg1	mice					746:749	WT mice	743:749	WT mice	743:749	In mice fed normo- and high-protein diets, GCN2 pathway in the liver is not activated in WT mice, leading to a similar metabolic profile with the one of KO mice.
31573843	5	21	theme	GCN2	697:700	arg1	pathway					702:708	GCN2 pathway	697:708	GCN2 pathway in the liver	697:721	In mice fed normo- and high-protein diets, GCN2 pathway in the liver is not activated in WT mice, leading to a similar metabolic profile with the one of KO mice.
31573843	5	22	theme	normo-	666:671	arg1	diets					690:694	normo- and high-protein diets	666:694	normo- and high-protein diets	666:694	In mice fed normo- and high-protein diets, GCN2 pathway in the liver is not activated in WT mice, leading to a similar metabolic profile with the one of KO mice.
31573843	7	23	theme	postprandial	986:997	arg1	profile					1009:1015	a different postprandial oxidation profile	974:1015	a different postprandial oxidation profile compared with KO mice	974:1037	In turn, FGF21 maintains a high level of lipid oxidation, leading to a different postprandial oxidation profile compared with KO mice.
31573843	0	24	theme	Liver	0:4	arg1	GCN2					6:9	Liver GCN2	0:9	Liver GCN2	0:9	Liver GCN2 controls hepatic FGF21 secretion and modulates whole body postprandial oxidation profile under a low-protein diet.
31573843	1	25	theme	protein	249:255	arg1	synthesis					257:265	protein synthesis	249:265	protein synthesis	249:265	General control nonderepressible 2 (GCN2) is a kinase that detects amino acid deficiency and is involved in the control of protein synthesis and energy metabolism.
31573843	5	26	with	profile	783:789	arg1	one					800:802	one	800:802	one	800:802	In mice fed normo- and high-protein diets, GCN2 pathway in the liver is not activated in WT mice, leading to a similar metabolic profile with the one of KO mice.
31573843	5	26	with	profile	783:789	arg1	mice					810:813	KO mice	807:813	KO mice	807:813	In mice fed normo- and high-protein diets, GCN2 pathway in the liver is not activated in WT mice, leading to a similar metabolic profile with the one of KO mice.
31573843	0	27	theme	hepatic	20:26	arg1	secretion					34:42	hepatic FGF21 secretion	20:42	hepatic FGF21 secretion	20:42	Liver GCN2 controls hepatic FGF21 secretion and modulates whole body postprandial oxidation profile under a low-protein diet.
31573843	1	28	theme	synthesis	257:265	arg1	control					238:244	the control	234:244	the control of protein synthesis and energy metabolism	234:287	General control nonderepressible 2 (GCN2) is a kinase that detects amino acid deficiency and is involved in the control of protein synthesis and energy metabolism.
31573843	5	29	theme	KO	807:808	arg1	mice					810:813	KO mice	807:813	KO mice	807:813	In mice fed normo- and high-protein diets, GCN2 pathway in the liver is not activated in WT mice, leading to a similar metabolic profile with the one of KO mice.
31573843	2	30	theme	dietary	386:392	arg1	protein					394:400	dietary protein	386:400	dietary protein	386:400	However, the role of hepatic GCN2 in the metabolic adaptations in response to the modulation of dietary protein has been seldom studied.
31573843	2	31	theme	protein	394:400	arg1	modulation					372:381	the modulation	368:381	the modulation of dietary protein	368:400	However, the role of hepatic GCN2 in the metabolic adaptations in response to the modulation of dietary protein has been seldom studied.
31573843	3	32	theme	normo-protein	495:507	arg1	diet					509:512	a normo-protein diet	493:512	a normo-protein diet	493:512	Wild-type (WT) and liver GCN2-deficient (KO) mice were fed either a normo-protein diet, a low-protein diet, or a high-protein diet for 3 wk.
31573843	3	33	theme	KO	468:469	arg1	mice					472:475	GCN2-deficient (KO) mice	452:475	GCN2-deficient (KO) mice	452:475	Wild-type (WT) and liver GCN2-deficient (KO) mice were fed either a normo-protein diet, a low-protein diet, or a high-protein diet for 3 wk.
31573843	1	34	theme	energy	271:276	arg1	metabolism					278:287	energy metabolism	271:287	energy metabolism	271:287	General control nonderepressible 2 (GCN2) is a kinase that detects amino acid deficiency and is involved in the control of protein synthesis and energy metabolism.
31573843	8	35	theme	body	1125:1128	arg1	profile					1153:1159	a whole body postprandial oxidation profile	1117:1159	a whole body postprandial oxidation profile	1117:1159	Hepatic GCN2 controls FGF21 secretion under a low-protein diet and modulates a whole body postprandial oxidation profile.
31573843	1	36	theme	amino	193:197	arg1	deficiency					204:213	amino acid deficiency	193:213	amino acid deficiency	193:213	General control nonderepressible 2 (GCN2) is a kinase that detects amino acid deficiency and is involved in the control of protein synthesis and energy metabolism.
31573843	0	37	theme	FGF21	28:32	arg1	secretion					34:42	hepatic FGF21 secretion	20:42	hepatic FGF21 secretion	20:42	Liver GCN2 controls hepatic FGF21 secretion and modulates whole body postprandial oxidation profile under a low-protein diet.
31573843	1	38	theme	metabolism	278:287	arg1	control					238:244	the control	234:244	the control of protein synthesis and energy metabolism	234:287	General control nonderepressible 2 (GCN2) is a kinase that detects amino acid deficiency and is involved in the control of protein synthesis and energy metabolism.
31573843	5	39	theme	high-protein	677:688	arg1	diets					690:694	normo- and high-protein diets	666:694	normo- and high-protein diets	666:694	In mice fed normo- and high-protein diets, GCN2 pathway in the liver is not activated in WT mice, leading to a similar metabolic profile with the one of KO mice.
31573843	8	40	theme	postprandial	1130:1141	arg1	profile					1153:1159	a whole body postprandial oxidation profile	1117:1159	a whole body postprandial oxidation profile	1117:1159	Hepatic GCN2 controls FGF21 secretion under a low-protein diet and modulates a whole body postprandial oxidation profile.
31573843	1	41	theme	acid	199:202	arg1	deficiency					204:213	amino acid deficiency	193:213	amino acid deficiency	193:213	General control nonderepressible 2 (GCN2) is a kinase that detects amino acid deficiency and is involved in the control of protein synthesis and energy metabolism.
31573843	7	42	theme	oxidation	952:960	arg1	level					937:941	a high level	930:941	a high level of lipid oxidation	930:960	In turn, FGF21 maintains a high level of lipid oxidation, leading to a different postprandial oxidation profile compared with KO mice.
31573843	2	43	from	adaptations	341:351	arg1	response					356:363	response	356:363	response to the modulation of dietary protein	356:400	However, the role of hepatic GCN2 in the metabolic adaptations in response to the modulation of dietary protein has been seldom studied.
31573843	2	44	theme	hepatic	311:317	arg1	GCN2					319:322	hepatic GCN2	311:322	hepatic GCN2	311:322	However, the role of hepatic GCN2 in the metabolic adaptations in response to the modulation of dietary protein has been seldom studied.
31573843	8	45	theme	Hepatic	1040:1046	arg1	GCN2					1048:1051	Hepatic GCN2	1040:1051	Hepatic GCN2	1040:1051	Hepatic GCN2 controls FGF21 secretion under a low-protein diet and modulates a whole body postprandial oxidation profile.
31573843	3	46	theme	low-protein	517:527	arg1	diet					529:532	a low-protein diet	515:532	a low-protein diet	515:532	Wild-type (WT) and liver GCN2-deficient (KO) mice were fed either a normo-protein diet, a low-protein diet, or a high-protein diet for 3 wk.
31573843	3	47	theme	high-protein	540:551	arg1	diet					553:556	a high-protein diet	538:556	a high-protein diet	538:556	Wild-type (WT) and liver GCN2-deficient (KO) mice were fed either a normo-protein diet, a low-protein diet, or a high-protein diet for 3 wk.
31573843	1	48	theme	General	126:132	arg1	kinase					173:178	a kinase	171:178	a kinase that detects amino acid deficiency and is involved in the control of protein synthesis and energy metabolism	171:287	General control nonderepressible 2 (GCN2) is a kinase that detects amino acid deficiency and is involved in the control of protein synthesis and energy metabolism.
31573843	1	48	theme	General	126:132	arg1	nonderepressible					142:157	General control nonderepressible 2	126:159	General control nonderepressible 2 (GCN2)	126:166	General control nonderepressible 2 (GCN2) is a kinase that detects amino acid deficiency and is involved in the control of protein synthesis and energy metabolism.
31573843	1	48	theme	General	126:132	arg1	GCN2					162:165	GCN2	162:165	GCN2	162:165	General control nonderepressible 2 (GCN2) is a kinase that detects amino acid deficiency and is involved in the control of protein synthesis and energy metabolism.
31573843	6	49	theme	WT	870:871	arg1	mice					873:876	WT mice	870:876	WT mice	870:876	On the contrary, a low-protein diet activates GCN2 in WT mice, inducing FGF21 secretion.
31573843	8	50	theme	oxidation	1143:1151	arg1	profile					1153:1159	a whole body postprandial oxidation profile	1117:1159	a whole body postprandial oxidation profile	1117:1159	Hepatic GCN2 controls FGF21 secretion under a low-protein diet and modulates a whole body postprandial oxidation profile.
31573843	1	51	theme	control	134:140	arg1	kinase					173:178	a kinase	171:178	a kinase that detects amino acid deficiency and is involved in the control of protein synthesis and energy metabolism	171:287	General control nonderepressible 2 (GCN2) is a kinase that detects amino acid deficiency and is involved in the control of protein synthesis and energy metabolism.
31573843	1	51	theme	control	134:140	arg1	nonderepressible					142:157	General control nonderepressible 2	126:159	General control nonderepressible 2 (GCN2)	126:166	General control nonderepressible 2 (GCN2) is a kinase that detects amino acid deficiency and is involved in the control of protein synthesis and energy metabolism.
31573843	1	51	theme	control	134:140	arg1	GCN2					162:165	GCN2	162:165	GCN2	162:165	General control nonderepressible 2 (GCN2) is a kinase that detects amino acid deficiency and is involved in the control of protein synthesis and energy metabolism.
31573843	4	52	theme	metabolic	618:626	arg1	parameters					628:637	metabolic parameters	618:637	metabolic parameters	618:637	During this period, body weight, food intake, and metabolic parameters were followed.
31573843	0	53	theme	body	64:67	arg1	profile					92:98	whole body postprandial oxidation profile	58:98	whole body postprandial oxidation profile under a low-protein diet	58:123	Liver GCN2 controls hepatic FGF21 secretion and modulates whole body postprandial oxidation profile under a low-protein diet.
31573843	3	54	dep	Wild-type	427:435	arg1	WT					438:439	WT	438:439	WT	438:439	Wild-type (WT) and liver GCN2-deficient (KO) mice were fed either a normo-protein diet, a low-protein diet, or a high-protein diet for 3 wk.
31573843	3	54	dep	Wild-type	427:435	arg1	mice					472:475	GCN2-deficient (KO) mice	452:475	GCN2-deficient (KO) mice	452:475	Wild-type (WT) and liver GCN2-deficient (KO) mice were fed either a normo-protein diet, a low-protein diet, or a high-protein diet for 3 wk.
31573843	6	55	theme	FGF21	888:892	arg1	secretion					894:902	FGF21 secretion	888:902	FGF21 secretion	888:902	On the contrary, a low-protein diet activates GCN2 in WT mice, inducing FGF21 secretion.
31573843	2	56	theme	metabolic	331:339	arg1	adaptations					341:351	the metabolic adaptations	327:351	the metabolic adaptations in response to the modulation of dietary protein	327:400	However, the role of hepatic GCN2 in the metabolic adaptations in response to the modulation of dietary protein has been seldom studied.
31573843	0	57	theme	whole	58:62	arg1	profile					92:98	whole body postprandial oxidation profile	58:98	whole body postprandial oxidation profile under a low-protein diet	58:123	Liver GCN2 controls hepatic FGF21 secretion and modulates whole body postprandial oxidation profile under a low-protein diet.
31573843	7	58	theme	different	976:984	arg1	profile					1009:1015	a different postprandial oxidation profile	974:1015	a different postprandial oxidation profile compared with KO mice	974:1037	In turn, FGF21 maintains a high level of lipid oxidation, leading to a different postprandial oxidation profile compared with KO mice.
31573843	4	59	theme	body	588:591	arg1	weight					593:598	body weight	588:598	body weight	588:598	During this period, body weight, food intake, and metabolic parameters were followed.
31486765	10	0	theme	thalictri	1479:1487	arg1	sp					1489:1490	the name Paenibacillus thalictri sp	1456:1490	the name Paenibacillus thalictri sp	1456:1490	Based on the results of phylogenetic, phenotypic and chemotaxonomic analysis, strain N2SHLJ1T was clearly distinguished from other species with validly published names in the genus Paenibacillus and should therefore be designated as a novel species, for which the name Paenibacillus thalictri sp.
31486765	6	1	contain	contained	740:748	arg2	menaquinone					795:805	the predominant menaquinone	779:805	the predominant menaquinone was MK-7	779:814	The cell-wall peptidoglycan contained meso-diaminopimelic acid and the predominant menaquinone was MK-7.
31486765	6	1	contain	contained	740:748	arg1	peptidoglycan					726:738	The cell-wall peptidoglycan	712:738	The cell-wall peptidoglycan	712:738	The cell-wall peptidoglycan contained meso-diaminopimelic acid and the predominant menaquinone was MK-7.
31486765	6	1	contain	contained	740:748	arg2	acid					770:773	meso-diaminopimelic acid	750:773	meso-diaminopimelic acid	750:773	The cell-wall peptidoglycan contained meso-diaminopimelic acid and the predominant menaquinone was MK-7.
31486765	7	2	dep	anteiso-C15 	844:855	arg1	iso-C15 					864:871	iso-C15 	864:871	iso-C15 	864:871	The major fatty acids were anteiso-C15 : 0 and iso-C15 : 0.
31486765	7	2	dep	anteiso-C15 	844:855	arg1	 0					857:858	 0	857:858	 0	857:858	The major fatty acids were anteiso-C15 : 0 and iso-C15 : 0.
31486765	7	2	dep	anteiso-C15 	844:855	arg1	 0					873:874	 0	873:874	anteiso-C15 : 0 and iso-C15 : 0	844:874	The major fatty acids were anteiso-C15 : 0 and iso-C15 : 0.
31486765	4	3	theme	%	577:577	arg1	similarity					579:588	94.7 % similarity	572:588	94.7 % similarity	572:588	Phylogenetic analysis based on 16S rRNA gene sequencing suggested that strain N2SHLJ1T belongs to the genus Paenibacillus and is most closely to Paenibacillus aestuarii CJ25T (94.7 % similarity).
31486765	4	3	theme	%	577:577	arg1	CJ25T					565:569	Paenibacillus aestuarii CJ25T	541:569	Paenibacillus aestuarii CJ25T (94.7 % similarity)	541:589	Phylogenetic analysis based on 16S rRNA gene sequencing suggested that strain N2SHLJ1T belongs to the genus Paenibacillus and is most closely to Paenibacillus aestuarii CJ25T (94.7 % similarity).
31486765	8	4	theme	unidentified	1108:1119	arg1	glycolipid					1121:1130	an unidentified glycolipid	1105:1130	an unidentified glycolipid	1105:1130	The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, an unidentified aminophospholipid, an unidentified phospholipid, an unidentified aminolipid, an unidentified glycolipid and two unidentified lipids.
31486765	4	5	theme	94.7 	572:576	arg1	%					577:577	%	577:577	%	577:577	Phylogenetic analysis based on 16S rRNA gene sequencing suggested that strain N2SHLJ1T belongs to the genus Paenibacillus and is most closely to Paenibacillus aestuarii CJ25T (94.7 % similarity).
31486765	2	6	theme	Gram-stain-positive	101:119	arg1	bacterium					161:169	A Gram-stain-positive, rod-shaped, aerobic, endospore-forming bacterium	99:169	A Gram-stain-positive, rod-shaped, aerobic, endospore-forming bacterium	99:169	A Gram-stain-positive, rod-shaped, aerobic, endospore-forming bacterium, designated strain N2SHLJ1T, was isolated from a surface-sterilized tissue sample of Thalictrum simplex L. in Guizhou, PR China.
31486765	5	7	theme	Strain	592:597	arg1	N2SHLJ1T					599:606	Strain N2SHLJ1T	592:606	Strain N2SHLJ1T	592:606	Strain N2SHLJ1T grew at 10-37 °C (optimum, 30 °C), in 0-2 % (w/v) NaCl (optimum, 0 %) and in pH 5.0-8.0 (optimum, 7.0).
31486765	1	8	theme	surface-sterilized	48:65	arg1	tissue					67:72	surface-sterilized tissue	48:72	surface-sterilized tissue of Thalictrum simplex L	48:96	nov., isolated from surface-sterilized tissue of Thalictrum simplex L.
31486765	9	9	theme	DNA	1165:1167	arg1	content					1173:1179	The DNA G+C content	1161:1179	The DNA G+C content	1161:1179	The DNA G+C content was 50.8 mol%.
31486765	9	9	theme	DNA	1165:1167	arg1	%					1193:1193	50.8 mol%	1185:1193	50.8 mol%	1185:1193	The DNA G+C content was 50.8 mol%.
31486765	0	10	theme	Paenibacillus	0:12	arg1	sp					24:25	Paenibacillus thalictri sp	0:25	Paenibacillus thalictri sp.	0:26	Paenibacillus thalictri sp.
31486765	10	11	theme	analysis	1264:1271	arg1	results					1209:1215	the results	1205:1215	the results of phylogenetic, phenotypic and chemotaxonomic analysis	1205:1271	Based on the results of phylogenetic, phenotypic and chemotaxonomic analysis, strain N2SHLJ1T was clearly distinguished from other species with validly published names in the genus Paenibacillus and should therefore be designated as a novel species, for which the name Paenibacillus thalictri sp.
31486765	4	12	theme	strain	467:472	arg1	N2SHLJ1T					474:481	strain N2SHLJ1T	467:481	strain N2SHLJ1T	467:481	Phylogenetic analysis based on 16S rRNA gene sequencing suggested that strain N2SHLJ1T belongs to the genus Paenibacillus and is most closely to Paenibacillus aestuarii CJ25T (94.7 % similarity).
31486765	4	13	theme	Phylogenetic	396:407	arg1	analysis					409:416	Phylogenetic analysis	396:416	Phylogenetic analysis based on 16S rRNA gene sequencing	396:450	Phylogenetic analysis based on 16S rRNA gene sequencing suggested that strain N2SHLJ1T belongs to the genus Paenibacillus and is most closely to Paenibacillus aestuarii CJ25T (94.7 % similarity).
31486765	2	14	theme	Guizhou	281:287	arg1	China					293:297	Guizhou, PR China	281:297	China	293:297	A Gram-stain-positive, rod-shaped, aerobic, endospore-forming bacterium, designated strain N2SHLJ1T, was isolated from a surface-sterilized tissue sample of Thalictrum simplex L. in Guizhou, PR China.
31486765	10	15	theme	chemotaxonomic	1249:1262	arg1	analysis					1264:1271	phylogenetic, phenotypic and chemotaxonomic analysis	1220:1271	phylogenetic, phenotypic and chemotaxonomic analysis	1220:1271	Based on the results of phylogenetic, phenotypic and chemotaxonomic analysis, strain N2SHLJ1T was clearly distinguished from other species with validly published names in the genus Paenibacillus and should therefore be designated as a novel species, for which the name Paenibacillus thalictri sp.
31486765	2	16	attach	isolated	204:211	arg2	bacterium					161:169	A Gram-stain-positive, rod-shaped, aerobic, endospore-forming bacterium	99:169	A Gram-stain-positive, rod-shaped, aerobic, endospore-forming bacterium	99:169	A Gram-stain-positive, rod-shaped, aerobic, endospore-forming bacterium, designated strain N2SHLJ1T, was isolated from a surface-sterilized tissue sample of Thalictrum simplex L. in Guizhou, PR China.
31486765	2	16	attach	isolated	204:211	arg1	sample					246:251	a surface-sterilized tissue sample	218:251	a surface-sterilized tissue sample of Thalictrum simplex L. in Guizhou, PR China	218:297	A Gram-stain-positive, rod-shaped, aerobic, endospore-forming bacterium, designated strain N2SHLJ1T, was isolated from a surface-sterilized tissue sample of Thalictrum simplex L. in Guizhou, PR China.
31486765	8	17	theme	unidentified	1140:1151	arg1	lipids					1153:1158	two unidentified lipids	1136:1158	two unidentified lipids	1136:1158	The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, an unidentified aminophospholipid, an unidentified phospholipid, an unidentified aminolipid, an unidentified glycolipid and two unidentified lipids.
31486765	1	18	theme	Thalictrum	77:86	arg1	L					96:96	Thalictrum simplex L	77:96	Thalictrum simplex L	77:96	nov., isolated from surface-sterilized tissue of Thalictrum simplex L.
31486765	2	19	from	sample	246:251	arg1	China					293:297	Guizhou, PR China	281:297	China	293:297	A Gram-stain-positive, rod-shaped, aerobic, endospore-forming bacterium, designated strain N2SHLJ1T, was isolated from a surface-sterilized tissue sample of Thalictrum simplex L. in Guizhou, PR China.
31486765	0	20	theme	thalictri	14:22	arg1	sp					24:25	Paenibacillus thalictri sp	0:25	Paenibacillus thalictri sp.	0:26	Paenibacillus thalictri sp.
31486765	5	21	dep	5.0-8.0	688:694	arg1	optimum					697:703	optimum	697:703	optimum	697:703	Strain N2SHLJ1T grew at 10-37 °C (optimum, 30 °C), in 0-2 % (w/v) NaCl (optimum, 0 %) and in pH 5.0-8.0 (optimum, 7.0).
31486765	1	22	theme	simplex	88:94	arg1	L					96:96	Thalictrum simplex L	77:96	Thalictrum simplex L	77:96	nov., isolated from surface-sterilized tissue of Thalictrum simplex L.
31486765	10	23	theme	phenotypic	1234:1243	arg1	analysis					1264:1271	phylogenetic, phenotypic and chemotaxonomic analysis	1220:1271	phylogenetic, phenotypic and chemotaxonomic analysis	1220:1271	Based on the results of phylogenetic, phenotypic and chemotaxonomic analysis, strain N2SHLJ1T was clearly distinguished from other species with validly published names in the genus Paenibacillus and should therefore be designated as a novel species, for which the name Paenibacillus thalictri sp.
31486765	3	24	theme	polyphasic	375:384	arg1	approach					386:393	a polyphasic approach	373:393	a polyphasic approach	373:393	To determine the bacterium's taxonomic position, it was characterized by a polyphasic approach.
31486765	4	25	theme	aestuarii	555:563	arg1	similarity					579:588	94.7 % similarity	572:588	94.7 % similarity	572:588	Phylogenetic analysis based on 16S rRNA gene sequencing suggested that strain N2SHLJ1T belongs to the genus Paenibacillus and is most closely to Paenibacillus aestuarii CJ25T (94.7 % similarity).
31486765	4	25	theme	aestuarii	555:563	arg1	CJ25T					565:569	Paenibacillus aestuarii CJ25T	541:569	Paenibacillus aestuarii CJ25T (94.7 % similarity)	541:589	Phylogenetic analysis based on 16S rRNA gene sequencing suggested that strain N2SHLJ1T belongs to the genus Paenibacillus and is most closely to Paenibacillus aestuarii CJ25T (94.7 % similarity).
31486765	6	26	theme	cell-wall	716:724	arg1	peptidoglycan					726:738	The cell-wall peptidoglycan	712:738	The cell-wall peptidoglycan	712:738	The cell-wall peptidoglycan contained meso-diaminopimelic acid and the predominant menaquinone was MK-7.
31486765	9	27	theme	50.8 mol	1185:1192	arg1	content					1173:1179	The DNA G+C content	1161:1179	The DNA G+C content	1161:1179	The DNA G+C content was 50.8 mol%.
31486765	9	27	theme	50.8 mol	1185:1192	arg1	%					1193:1193	50.8 mol%	1185:1193	50.8 mol%	1185:1193	The DNA G+C content was 50.8 mol%.
31486765	1	28	theme	L	96:96	arg1	tissue					67:72	surface-sterilized tissue	48:72	surface-sterilized tissue of Thalictrum simplex L	48:96	nov., isolated from surface-sterilized tissue of Thalictrum simplex L.
31486765	4	29	theme	gene	436:439	arg1	sequencing					441:450	16S rRNA gene sequencing	427:450	16S rRNA gene sequencing	427:450	Phylogenetic analysis based on 16S rRNA gene sequencing suggested that strain N2SHLJ1T belongs to the genus Paenibacillus and is most closely to Paenibacillus aestuarii CJ25T (94.7 % similarity).
31486765	4	30	theme	Paenibacillus	541:553	arg1	similarity					579:588	94.7 % similarity	572:588	94.7 % similarity	572:588	Phylogenetic analysis based on 16S rRNA gene sequencing suggested that strain N2SHLJ1T belongs to the genus Paenibacillus and is most closely to Paenibacillus aestuarii CJ25T (94.7 % similarity).
31486765	4	30	theme	Paenibacillus	541:553	arg1	CJ25T					565:569	Paenibacillus aestuarii CJ25T	541:569	Paenibacillus aestuarii CJ25T (94.7 % similarity)	541:589	Phylogenetic analysis based on 16S rRNA gene sequencing suggested that strain N2SHLJ1T belongs to the genus Paenibacillus and is most closely to Paenibacillus aestuarii CJ25T (94.7 % similarity).
31486765	5	31	theme	w/v	653:655	arg1	optimum					664:670	optimum	664:670	optimum	664:670	Strain N2SHLJ1T grew at 10-37 °C (optimum, 30 °C), in 0-2 % (w/v) NaCl (optimum, 0 %) and in pH 5.0-8.0 (optimum, 7.0).
31486765	5	31	theme	w/v	653:655	arg1	NaCl					658:661	0-2 % (w/v) NaCl	646:661	0-2 % (w/v) NaCl (optimum, 0 %)	646:676	Strain N2SHLJ1T grew at 10-37 °C (optimum, 30 °C), in 0-2 % (w/v) NaCl (optimum, 0 %) and in pH 5.0-8.0 (optimum, 7.0).
31486765	8	32	theme	polar	881:885	arg1	lipids					887:892	The polar lipids	877:892	The polar lipids	877:892	The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, an unidentified aminophospholipid, an unidentified phospholipid, an unidentified aminolipid, an unidentified glycolipid and two unidentified lipids.
31486765	10	33	theme	novel	1431:1435	arg1	species					1437:1443	a novel species	1429:1443	a novel species	1429:1443	Based on the results of phylogenetic, phenotypic and chemotaxonomic analysis, strain N2SHLJ1T was clearly distinguished from other species with validly published names in the genus Paenibacillus and should therefore be designated as a novel species, for which the name Paenibacillus thalictri sp.
31486765	10	33	theme	novel	1431:1435	arg1	N2SHLJ1T					1281:1288	strain N2SHLJ1T	1274:1288	strain N2SHLJ1T	1274:1288	Based on the results of phylogenetic, phenotypic and chemotaxonomic analysis, strain N2SHLJ1T was clearly distinguished from other species with validly published names in the genus Paenibacillus and should therefore be designated as a novel species, for which the name Paenibacillus thalictri sp.
31486765	5	34	dep	pH	685:686	arg1	5.0-8.0					688:694	5.0-8.0	688:694	5.0-8.0	688:694	Strain N2SHLJ1T grew at 10-37 °C (optimum, 30 °C), in 0-2 % (w/v) NaCl (optimum, 0 %) and in pH 5.0-8.0 (optimum, 7.0).
31486765	6	35	theme	predominant	783:793	arg1	menaquinone					795:805	the predominant menaquinone	779:805	the predominant menaquinone was MK-7	779:814	The cell-wall peptidoglycan contained meso-diaminopimelic acid and the predominant menaquinone was MK-7.
31486765	10	36	theme	strain	1274:1279	arg1	species					1437:1443	a novel species	1429:1443	a novel species	1429:1443	Based on the results of phylogenetic, phenotypic and chemotaxonomic analysis, strain N2SHLJ1T was clearly distinguished from other species with validly published names in the genus Paenibacillus and should therefore be designated as a novel species, for which the name Paenibacillus thalictri sp.
31486765	10	36	theme	strain	1274:1279	arg1	N2SHLJ1T					1281:1288	strain N2SHLJ1T	1274:1288	strain N2SHLJ1T	1274:1288	Based on the results of phylogenetic, phenotypic and chemotaxonomic analysis, strain N2SHLJ1T was clearly distinguished from other species with validly published names in the genus Paenibacillus and should therefore be designated as a novel species, for which the name Paenibacillus thalictri sp.
31486765	10	37	from	names	1358:1362	arg1	Paenibacillus					1377:1389	Paenibacillus	1377:1389	Paenibacillus	1377:1389	Based on the results of phylogenetic, phenotypic and chemotaxonomic analysis, strain N2SHLJ1T was clearly distinguished from other species with validly published names in the genus Paenibacillus and should therefore be designated as a novel species, for which the name Paenibacillus thalictri sp.
31486765	2	38	theme	strain	183:188	arg1	N2SHLJ1T					190:197	strain N2SHLJ1T	183:197	strain N2SHLJ1T	183:197	A Gram-stain-positive, rod-shaped, aerobic, endospore-forming bacterium, designated strain N2SHLJ1T, was isolated from a surface-sterilized tissue sample of Thalictrum simplex L. in Guizhou, PR China.
31486765	5	39	dep	optimum	626:632	arg1	30 °C					635:639	30 °C	635:639	30 °C	635:639	Strain N2SHLJ1T grew at 10-37 °C (optimum, 30 °C), in 0-2 % (w/v) NaCl (optimum, 0 %) and in pH 5.0-8.0 (optimum, 7.0).
31486765	12	40	theme	=JCM	1540:1543	arg1	1.13865T					1558:1565	=JCM 33293T=CGMCC 1.13865T	1540:1565	=JCM 33293T=CGMCC 1.13865T	1540:1565	The type strain is N2SHLJ1T (=JCM 33293T=CGMCC 1.13865T).
31486765	12	40	theme	=JCM	1540:1543	arg1	N2SHLJ1T					1530:1537	N2SHLJ1T	1530:1537	N2SHLJ1T (=JCM 33293T=CGMCC 1.13865T)	1530:1566	The type strain is N2SHLJ1T (=JCM 33293T=CGMCC 1.13865T).
31486765	8	41	theme	unidentified	1050:1061	arg1	phospholipid					1063:1074	an unidentified phospholipid	1047:1074	an unidentified phospholipid	1047:1074	The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, an unidentified aminophospholipid, an unidentified phospholipid, an unidentified aminolipid, an unidentified glycolipid and two unidentified lipids.
31486765	10	42	theme	phylogenetic	1220:1231	arg1	analysis					1264:1271	phylogenetic, phenotypic and chemotaxonomic analysis	1220:1271	phylogenetic, phenotypic and chemotaxonomic analysis	1220:1271	Based on the results of phylogenetic, phenotypic and chemotaxonomic analysis, strain N2SHLJ1T was clearly distinguished from other species with validly published names in the genus Paenibacillus and should therefore be designated as a novel species, for which the name Paenibacillus thalictri sp.
31486765	2	43	theme	tissue	239:244	arg1	sample					246:251	a surface-sterilized tissue sample	218:251	a surface-sterilized tissue sample of Thalictrum simplex L. in Guizhou, PR China	218:297	A Gram-stain-positive, rod-shaped, aerobic, endospore-forming bacterium, designated strain N2SHLJ1T, was isolated from a surface-sterilized tissue sample of Thalictrum simplex L. in Guizhou, PR China.
31486765	10	44	theme	other	1321:1325	arg1	species					1327:1333	other species	1321:1333	other species	1321:1333	Based on the results of phylogenetic, phenotypic and chemotaxonomic analysis, strain N2SHLJ1T was clearly distinguished from other species with validly published names in the genus Paenibacillus and should therefore be designated as a novel species, for which the name Paenibacillus thalictri sp.
31486765	2	45	dep	Gram-stain-positive	101:119	arg1	rod-shaped					122:131	rod-shaped	122:131	rod-shaped	122:131	A Gram-stain-positive, rod-shaped, aerobic, endospore-forming bacterium, designated strain N2SHLJ1T, was isolated from a surface-sterilized tissue sample of Thalictrum simplex L. in Guizhou, PR China.
31486765	2	45	dep	Gram-stain-positive	101:119	arg1	endospore-forming					143:159	endospore-forming	143:159	endospore-forming	143:159	A Gram-stain-positive, rod-shaped, aerobic, endospore-forming bacterium, designated strain N2SHLJ1T, was isolated from a surface-sterilized tissue sample of Thalictrum simplex L. in Guizhou, PR China.
31486765	2	45	dep	Gram-stain-positive	101:119	arg1	aerobic					134:140	aerobic	134:140	aerobic	134:140	A Gram-stain-positive, rod-shaped, aerobic, endospore-forming bacterium, designated strain N2SHLJ1T, was isolated from a surface-sterilized tissue sample of Thalictrum simplex L. in Guizhou, PR China.
31486765	2	46	theme	surface-sterilized	220:237	arg1	sample					246:251	a surface-sterilized tissue sample	218:251	a surface-sterilized tissue sample of Thalictrum simplex L. in Guizhou, PR China	218:297	A Gram-stain-positive, rod-shaped, aerobic, endospore-forming bacterium, designated strain N2SHLJ1T, was isolated from a surface-sterilized tissue sample of Thalictrum simplex L. in Guizhou, PR China.
31486765	5	47	dep	optimum	664:670	arg1	%					675:675	0 %	673:675	0 %	673:675	Strain N2SHLJ1T grew at 10-37 °C (optimum, 30 °C), in 0-2 % (w/v) NaCl (optimum, 0 %) and in pH 5.0-8.0 (optimum, 7.0).
31486765	4	48	theme	Paenibacillus	504:516	arg1	genus					498:502	the genus Paenibacillus	494:516	the genus Paenibacillus	494:516	Phylogenetic analysis based on 16S rRNA gene sequencing suggested that strain N2SHLJ1T belongs to the genus Paenibacillus and is most closely to Paenibacillus aestuarii CJ25T (94.7 % similarity).
31486765	10	49	theme	Paenibacillus	1465:1477	arg1	sp					1489:1490	the name Paenibacillus thalictri sp	1456:1490	the name Paenibacillus thalictri sp	1456:1490	Based on the results of phylogenetic, phenotypic and chemotaxonomic analysis, strain N2SHLJ1T was clearly distinguished from other species with validly published names in the genus Paenibacillus and should therefore be designated as a novel species, for which the name Paenibacillus thalictri sp.
31486765	6	50	theme	meso-diaminopimelic	750:768	arg1	acid					770:773	meso-diaminopimelic acid	750:773	meso-diaminopimelic acid	750:773	The cell-wall peptidoglycan contained meso-diaminopimelic acid and the predominant menaquinone was MK-7.
31486765	2	51	dep	China	293:297	arg1	PR					290:291	Guizhou, PR China	281:297	PR	290:291	A Gram-stain-positive, rod-shaped, aerobic, endospore-forming bacterium, designated strain N2SHLJ1T, was isolated from a surface-sterilized tissue sample of Thalictrum simplex L. in Guizhou, PR China.
31486765	7	52	theme	fatty	827:831	arg1	anteiso-C15 					844:855	anteiso-C15 	844:855	anteiso-C15 	844:855	The major fatty acids were anteiso-C15 : 0 and iso-C15 : 0.
31486765	7	52	theme	fatty	827:831	arg1	acids					833:837	The major fatty acids	817:837	The major fatty acids	817:837	The major fatty acids were anteiso-C15 : 0 and iso-C15 : 0.
31486765	12	53	theme	33293T=CGMCC	1545:1556	arg1	1.13865T					1558:1565	=JCM 33293T=CGMCC 1.13865T	1540:1565	=JCM 33293T=CGMCC 1.13865T	1540:1565	The type strain is N2SHLJ1T (=JCM 33293T=CGMCC 1.13865T).
31486765	12	53	theme	33293T=CGMCC	1545:1556	arg1	N2SHLJ1T					1530:1537	N2SHLJ1T	1530:1537	N2SHLJ1T (=JCM 33293T=CGMCC 1.13865T)	1530:1566	The type strain is N2SHLJ1T (=JCM 33293T=CGMCC 1.13865T).
31486765	5	54	theme	%	650:650	arg1	optimum					664:670	optimum	664:670	optimum	664:670	Strain N2SHLJ1T grew at 10-37 °C (optimum, 30 °C), in 0-2 % (w/v) NaCl (optimum, 0 %) and in pH 5.0-8.0 (optimum, 7.0).
31486765	5	54	theme	%	650:650	arg1	NaCl					658:661	0-2 % (w/v) NaCl	646:661	0-2 % (w/v) NaCl (optimum, 0 %)	646:676	Strain N2SHLJ1T grew at 10-37 °C (optimum, 30 °C), in 0-2 % (w/v) NaCl (optimum, 0 %) and in pH 5.0-8.0 (optimum, 7.0).
31486765	2	55	theme	L.	275:276	arg1	sample					246:251	a surface-sterilized tissue sample	218:251	a surface-sterilized tissue sample of Thalictrum simplex L. in Guizhou, PR China	218:297	A Gram-stain-positive, rod-shaped, aerobic, endospore-forming bacterium, designated strain N2SHLJ1T, was isolated from a surface-sterilized tissue sample of Thalictrum simplex L. in Guizhou, PR China.
31486765	8	56	theme	unidentified	1080:1091	arg1	aminolipid					1093:1102	an unidentified aminolipid	1077:1102	an unidentified aminolipid	1077:1102	The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, an unidentified aminophospholipid, an unidentified phospholipid, an unidentified aminolipid, an unidentified glycolipid and two unidentified lipids.
31486765	4	57	theme	rRNA	431:434	arg1	sequencing					441:450	16S rRNA gene sequencing	427:450	16S rRNA gene sequencing	427:450	Phylogenetic analysis based on 16S rRNA gene sequencing suggested that strain N2SHLJ1T belongs to the genus Paenibacillus and is most closely to Paenibacillus aestuarii CJ25T (94.7 % similarity).
31486765	2	58	theme	simplex	267:273	arg1	L.					275:276	Thalictrum simplex L.	256:276	Thalictrum simplex L.	256:276	A Gram-stain-positive, rod-shaped, aerobic, endospore-forming bacterium, designated strain N2SHLJ1T, was isolated from a surface-sterilized tissue sample of Thalictrum simplex L. in Guizhou, PR China.
31486765	10	59	theme	published	1348:1356	arg1	names					1358:1362	validly published names	1340:1362	validly published names in the genus Paenibacillus	1340:1389	Based on the results of phylogenetic, phenotypic and chemotaxonomic analysis, strain N2SHLJ1T was clearly distinguished from other species with validly published names in the genus Paenibacillus and should therefore be designated as a novel species, for which the name Paenibacillus thalictri sp.
31486765	9	60	theme	G+C	1169:1171	arg1	content					1173:1179	The DNA G+C content	1161:1179	The DNA G+C content	1161:1179	The DNA G+C content was 50.8 mol%.
31486765	9	60	theme	G+C	1169:1171	arg1	%					1193:1193	50.8 mol%	1185:1193	50.8 mol%	1185:1193	The DNA G+C content was 50.8 mol%.
31486765	12	61	theme	type	1515:1518	arg1	strain					1520:1525	The type strain	1511:1525	The type strain	1511:1525	The type strain is N2SHLJ1T (=JCM 33293T=CGMCC 1.13865T).
31486765	12	61	theme	type	1515:1518	arg1	N2SHLJ1T					1530:1537	N2SHLJ1T	1530:1537	N2SHLJ1T (=JCM 33293T=CGMCC 1.13865T)	1530:1566	The type strain is N2SHLJ1T (=JCM 33293T=CGMCC 1.13865T).
31486765	10	62	theme	name	1460:1463	arg1	sp					1489:1490	the name Paenibacillus thalictri sp	1456:1490	the name Paenibacillus thalictri sp	1456:1490	Based on the results of phylogenetic, phenotypic and chemotaxonomic analysis, strain N2SHLJ1T was clearly distinguished from other species with validly published names in the genus Paenibacillus and should therefore be designated as a novel species, for which the name Paenibacillus thalictri sp.
31486765	4	63	theme	16S	427:429	arg1	sequencing					441:450	16S rRNA gene sequencing	427:450	16S rRNA gene sequencing	427:450	Phylogenetic analysis based on 16S rRNA gene sequencing suggested that strain N2SHLJ1T belongs to the genus Paenibacillus and is most closely to Paenibacillus aestuarii CJ25T (94.7 % similarity).
31486765	2	64	theme	Thalictrum	256:265	arg1	L.					275:276	Thalictrum simplex L.	256:276	Thalictrum simplex L.	256:276	A Gram-stain-positive, rod-shaped, aerobic, endospore-forming bacterium, designated strain N2SHLJ1T, was isolated from a surface-sterilized tissue sample of Thalictrum simplex L. in Guizhou, PR China.
31486765	7	65	theme	major	821:825	arg1	anteiso-C15 					844:855	anteiso-C15 	844:855	anteiso-C15 	844:855	The major fatty acids were anteiso-C15 : 0 and iso-C15 : 0.
31486765	7	65	theme	major	821:825	arg1	acids					833:837	The major fatty acids	817:837	The major fatty acids	817:837	The major fatty acids were anteiso-C15 : 0 and iso-C15 : 0.
31486765	3	66	theme	taxonomic	329:337	arg1	position					339:346	the bacterium's taxonomic position	313:346	the bacterium's taxonomic position	313:346	To determine the bacterium's taxonomic position, it was characterized by a polyphasic approach.
31486765	5	67	theme	7.0	706:708	arg1	optimum					697:703	optimum	697:703	optimum	697:703	Strain N2SHLJ1T grew at 10-37 °C (optimum, 30 °C), in 0-2 % (w/v) NaCl (optimum, 0 %) and in pH 5.0-8.0 (optimum, 7.0).
30918252	0	0	theme	ganglioside-like	69:84	arg1	glycoconjugates					86:100	ganglioside-like glycoconjugates	69:100	ganglioside-like glycoconjugates	69:100	Bacterial AB5 toxins inhibit the growth of gut bacteria by targeting ganglioside-like glycoconjugates.
30918252	1	1	theme	intestinal	307:316	arg1	bacteria					318:325	other intestinal bacteria	301:325	other intestinal bacteria	301:325	The AB5 toxins cholera toxin (CT) from Vibrio cholerae and heat-labile enterotoxin (LT) from enterotoxigenic Escherichia coli are notorious for their roles in diarrheal disease, but their effect on other intestinal bacteria remains unexplored.
30918252	1	2	from	effect	291:296	arg1	bacteria					318:325	other intestinal bacteria	301:325	other intestinal bacteria	301:325	The AB5 toxins cholera toxin (CT) from Vibrio cholerae and heat-labile enterotoxin (LT) from enterotoxigenic Escherichia coli are notorious for their roles in diarrheal disease, but their effect on other intestinal bacteria remains unexplored.
30918252	2	3	dep	demonstrate	458:468	arg1	mimic					401:405	mimic	401:405	can mimic the GM1 ganglioside receptor of CT and LT.	397:448	Another foodborne pathogen, Campylobacter jejuni, can mimic the GM1 ganglioside receptor of CT and LT. Here we demonstrate that the toxin B-subunits (CTB and LTB) inhibit C. jejuni growth by binding to GM1-mimicking lipooligosaccharides and increasing permeability of the cell membrane.
30918252	3	4	theme	CTB	661:663	arg1	incubation					647:656	incubation	647:656	incubation of CTB or LTB with a C. jejuni isolate capable of altering its lipooligosaccharide structure	647:749	Furthermore, incubation of CTB or LTB with a C. jejuni isolate capable of altering its lipooligosaccharide structure selects for variants lacking the GM1 mimic.
30918252	2	5	theme	GM1-mimicking	549:561	arg1	lipooligosaccharides					563:582	GM1-mimicking lipooligosaccharides	549:582	GM1-mimicking lipooligosaccharides	549:582	Another foodborne pathogen, Campylobacter jejuni, can mimic the GM1 ganglioside receptor of CT and LT. Here we demonstrate that the toxin B-subunits (CTB and LTB) inhibit C. jejuni growth by binding to GM1-mimicking lipooligosaccharides and increasing permeability of the cell membrane.
30918252	1	6	theme	Vibrio	142:147	arg1	cholerae					149:156	Vibrio cholerae	142:156	Vibrio cholerae	142:156	The AB5 toxins cholera toxin (CT) from Vibrio cholerae and heat-labile enterotoxin (LT) from enterotoxigenic Escherichia coli are notorious for their roles in diarrheal disease, but their effect on other intestinal bacteria remains unexplored.
30918252	4	7	theme	commensal	940:948	arg1	bacteria					950:957	commensal bacteria	940:957	commensal bacteria	940:957	Examining the chicken GI tract with immunofluorescence microscopy demonstrates that GM1 reactive structures are abundant on epithelial cells and commensal bacteria, further emphasizing the relevance of this mimicry.
30918252	5	8	theme	toxin	1121:1125	arg1	functions					1127:1135	new toxin functions	1117:1135	new toxin functions in bacterial gut competition	1117:1164	Exposure of chickens to CTB or LTB causes shifts in the gut microbial composition, providing evidence for new toxin functions in bacterial gut competition.
30918252	5	9	from	functions	1127:1135	arg1	competition					1154:1164	bacterial gut competition	1140:1164	bacterial gut competition	1140:1164	Exposure of chickens to CTB or LTB causes shifts in the gut microbial composition, providing evidence for new toxin functions in bacterial gut competition.
30918252	2	10	theme	membrane	624:631	arg1	permeability					599:610	permeability	599:610	permeability of the cell membrane	599:631	Another foodborne pathogen, Campylobacter jejuni, can mimic the GM1 ganglioside receptor of CT and LT. Here we demonstrate that the toxin B-subunits (CTB and LTB) inhibit C. jejuni growth by binding to GM1-mimicking lipooligosaccharides and increasing permeability of the cell membrane.
30918252	5	11	theme	bacterial	1140:1148	arg1	competition					1154:1164	bacterial gut competition	1140:1164	bacterial gut competition	1140:1164	Exposure of chickens to CTB or LTB causes shifts in the gut microbial composition, providing evidence for new toxin functions in bacterial gut competition.
30918252	3	12	theme	jejuni	682:687	arg1	isolate					689:695	a C. jejuni isolate	677:695	a C. jejuni isolate capable of altering its lipooligosaccharide structure	677:749	Furthermore, incubation of CTB or LTB with a C. jejuni isolate capable of altering its lipooligosaccharide structure selects for variants lacking the GM1 mimic.
30918252	2	13	theme	cell	619:622	arg1	membrane					624:631	the cell membrane	615:631	the cell membrane	615:631	Another foodborne pathogen, Campylobacter jejuni, can mimic the GM1 ganglioside receptor of CT and LT. Here we demonstrate that the toxin B-subunits (CTB and LTB) inhibit C. jejuni growth by binding to GM1-mimicking lipooligosaccharides and increasing permeability of the cell membrane.
30918252	5	14	from	shifts	1053:1058	arg1	composition					1081:1091	the gut microbial composition	1063:1091	the gut microbial composition	1063:1091	Exposure of chickens to CTB or LTB causes shifts in the gut microbial composition, providing evidence for new toxin functions in bacterial gut competition.
30918252	4	15	theme	GI	817:818	arg1	tract					820:824	the chicken GI tract	805:824	the chicken GI tract	805:824	Examining the chicken GI tract with immunofluorescence microscopy demonstrates that GM1 reactive structures are abundant on epithelial cells and commensal bacteria, further emphasizing the relevance of this mimicry.
30918252	1	16	dep	toxins	111:116	arg1	toxin					126:130	cholera toxin	118:130	The AB5 toxins cholera toxin (CT) from Vibrio cholerae and heat-labile enterotoxin (LT) from enterotoxigenic Escherichia coli	103:227	The AB5 toxins cholera toxin (CT) from Vibrio cholerae and heat-labile enterotoxin (LT) from enterotoxigenic Escherichia coli are notorious for their roles in diarrheal disease, but their effect on other intestinal bacteria remains unexplored.
30918252	1	16	dep	toxins	111:116	arg1	CT					133:134	CT	133:134	CT	133:134	The AB5 toxins cholera toxin (CT) from Vibrio cholerae and heat-labile enterotoxin (LT) from enterotoxigenic Escherichia coli are notorious for their roles in diarrheal disease, but their effect on other intestinal bacteria remains unexplored.
30918252	5	17	theme	gut	1067:1069	arg1	composition					1081:1091	the gut microbial composition	1063:1091	the gut microbial composition	1063:1091	Exposure of chickens to CTB or LTB causes shifts in the gut microbial composition, providing evidence for new toxin functions in bacterial gut competition.
30918252	2	18	theme	toxin	479:483	arg1	B-subunits					485:494	the toxin B-subunits	475:494	the toxin B-subunits (CTB and LTB)	475:508	Another foodborne pathogen, Campylobacter jejuni, can mimic the GM1 ganglioside receptor of CT and LT. Here we demonstrate that the toxin B-subunits (CTB and LTB) inhibit C. jejuni growth by binding to GM1-mimicking lipooligosaccharides and increasing permeability of the cell membrane.
30918252	2	18	theme	toxin	479:483	arg1	LTB					505:507	LTB	505:507	LTB	505:507	Another foodborne pathogen, Campylobacter jejuni, can mimic the GM1 ganglioside receptor of CT and LT. Here we demonstrate that the toxin B-subunits (CTB and LTB) inhibit C. jejuni growth by binding to GM1-mimicking lipooligosaccharides and increasing permeability of the cell membrane.
30918252	2	18	theme	toxin	479:483	arg1	CTB					497:499	CTB	497:499	CTB	497:499	Another foodborne pathogen, Campylobacter jejuni, can mimic the GM1 ganglioside receptor of CT and LT. Here we demonstrate that the toxin B-subunits (CTB and LTB) inhibit C. jejuni growth by binding to GM1-mimicking lipooligosaccharides and increasing permeability of the cell membrane.
30918252	4	19	theme	chicken	809:815	arg1	tract					820:824	the chicken GI tract	805:824	the chicken GI tract	805:824	Examining the chicken GI tract with immunofluorescence microscopy demonstrates that GM1 reactive structures are abundant on epithelial cells and commensal bacteria, further emphasizing the relevance of this mimicry.
30918252	3	20	with	incubation	647:656	arg1	isolate					689:695	a C. jejuni isolate	677:695	a C. jejuni isolate capable of altering its lipooligosaccharide structure	677:749	Furthermore, incubation of CTB or LTB with a C. jejuni isolate capable of altering its lipooligosaccharide structure selects for variants lacking the GM1 mimic.
30918252	2	21	theme	Campylobacter	375:387	arg1	pathogen					365:372	Another foodborne pathogen	347:372	Another foodborne pathogen	347:372	Another foodborne pathogen, Campylobacter jejuni, can mimic the GM1 ganglioside receptor of CT and LT. Here we demonstrate that the toxin B-subunits (CTB and LTB) inhibit C. jejuni growth by binding to GM1-mimicking lipooligosaccharides and increasing permeability of the cell membrane.
30918252	2	21	theme	Campylobacter	375:387	arg1	jejuni					389:394	Campylobacter jejuni	375:394	Campylobacter jejuni	375:394	Another foodborne pathogen, Campylobacter jejuni, can mimic the GM1 ganglioside receptor of CT and LT. Here we demonstrate that the toxin B-subunits (CTB and LTB) inhibit C. jejuni growth by binding to GM1-mimicking lipooligosaccharides and increasing permeability of the cell membrane.
30918252	0	22	theme	AB5	10:12	arg1	toxins					14:19	Bacterial AB5 toxins	0:19	Bacterial AB5 toxins	0:19	Bacterial AB5 toxins inhibit the growth of gut bacteria by targeting ganglioside-like glycoconjugates.
30918252	3	23	theme	C.	679:680	arg1	isolate					689:695	a C. jejuni isolate	677:695	a C. jejuni isolate capable of altering its lipooligosaccharide structure	677:749	Furthermore, incubation of CTB or LTB with a C. jejuni isolate capable of altering its lipooligosaccharide structure selects for variants lacking the GM1 mimic.
30918252	4	24	theme	GM1	879:881	arg1	structures					892:901	GM1 reactive structures	879:901	GM1 reactive structures	879:901	Examining the chicken GI tract with immunofluorescence microscopy demonstrates that GM1 reactive structures are abundant on epithelial cells and commensal bacteria, further emphasizing the relevance of this mimicry.
30918252	1	25	theme	heat-labile	162:172	arg1	LT					187:188	LT	187:188	LT	187:188	The AB5 toxins cholera toxin (CT) from Vibrio cholerae and heat-labile enterotoxin (LT) from enterotoxigenic Escherichia coli are notorious for their roles in diarrheal disease, but their effect on other intestinal bacteria remains unexplored.
30918252	1	25	theme	heat-labile	162:172	arg1	enterotoxin					174:184	heat-labile enterotoxin	162:184	heat-labile enterotoxin (LT)	162:189	The AB5 toxins cholera toxin (CT) from Vibrio cholerae and heat-labile enterotoxin (LT) from enterotoxigenic Escherichia coli are notorious for their roles in diarrheal disease, but their effect on other intestinal bacteria remains unexplored.
30918252	0	26	theme	Bacterial	0:8	arg1	toxins					14:19	Bacterial AB5 toxins	0:19	Bacterial AB5 toxins	0:19	Bacterial AB5 toxins inhibit the growth of gut bacteria by targeting ganglioside-like glycoconjugates.
30918252	4	27	theme	immunofluorescence	831:848	arg1	microscopy					850:859	immunofluorescence microscopy	831:859	immunofluorescence microscopy	831:859	Examining the chicken GI tract with immunofluorescence microscopy demonstrates that GM1 reactive structures are abundant on epithelial cells and commensal bacteria, further emphasizing the relevance of this mimicry.
30918252	2	28	theme	ganglioside	415:425	arg1	receptor					427:434	the GM1 ganglioside receptor	407:434	the GM1 ganglioside receptor of CT and LT.	407:448	Another foodborne pathogen, Campylobacter jejuni, can mimic the GM1 ganglioside receptor of CT and LT. Here we demonstrate that the toxin B-subunits (CTB and LTB) inhibit C. jejuni growth by binding to GM1-mimicking lipooligosaccharides and increasing permeability of the cell membrane.
30918252	1	29	theme	diarrheal	262:270	arg1	disease					272:278	diarrheal disease	262:278	diarrheal disease	262:278	The AB5 toxins cholera toxin (CT) from Vibrio cholerae and heat-labile enterotoxin (LT) from enterotoxigenic Escherichia coli are notorious for their roles in diarrheal disease, but their effect on other intestinal bacteria remains unexplored.
30918252	2	30	dep	B-subunits	485:494	arg1	B-subunits					485:494	the toxin B-subunits	475:494	the toxin B-subunits (CTB and LTB)	475:508	Another foodborne pathogen, Campylobacter jejuni, can mimic the GM1 ganglioside receptor of CT and LT. Here we demonstrate that the toxin B-subunits (CTB and LTB) inhibit C. jejuni growth by binding to GM1-mimicking lipooligosaccharides and increasing permeability of the cell membrane.
30918252	2	30	dep	B-subunits	485:494	arg1	LTB					505:507	LTB	505:507	LTB	505:507	Another foodborne pathogen, Campylobacter jejuni, can mimic the GM1 ganglioside receptor of CT and LT. Here we demonstrate that the toxin B-subunits (CTB and LTB) inhibit C. jejuni growth by binding to GM1-mimicking lipooligosaccharides and increasing permeability of the cell membrane.
30918252	2	30	dep	B-subunits	485:494	arg1	CTB					497:499	CTB	497:499	CTB	497:499	Another foodborne pathogen, Campylobacter jejuni, can mimic the GM1 ganglioside receptor of CT and LT. Here we demonstrate that the toxin B-subunits (CTB and LTB) inhibit C. jejuni growth by binding to GM1-mimicking lipooligosaccharides and increasing permeability of the cell membrane.
30918252	2	31	theme	GM1	411:413	arg1	ganglioside					415:425	GM1 ganglioside	411:425	the GM1 ganglioside receptor of CT and LT.	407:448	Another foodborne pathogen, Campylobacter jejuni, can mimic the GM1 ganglioside receptor of CT and LT. Here we demonstrate that the toxin B-subunits (CTB and LTB) inhibit C. jejuni growth by binding to GM1-mimicking lipooligosaccharides and increasing permeability of the cell membrane.
30918252	3	32	theme	capable	697:703	arg1	isolate					689:695	a C. jejuni isolate	677:695	a C. jejuni isolate capable of altering its lipooligosaccharide structure	677:749	Furthermore, incubation of CTB or LTB with a C. jejuni isolate capable of altering its lipooligosaccharide structure selects for variants lacking the GM1 mimic.
30918252	1	33	from	roles	253:257	arg1	disease					272:278	diarrheal disease	262:278	diarrheal disease	262:278	The AB5 toxins cholera toxin (CT) from Vibrio cholerae and heat-labile enterotoxin (LT) from enterotoxigenic Escherichia coli are notorious for their roles in diarrheal disease, but their effect on other intestinal bacteria remains unexplored.
30918252	1	34	theme	AB5	107:109	arg1	toxins					111:116	The AB5 toxins cholera toxin (CT)	103:135	The AB5 toxins cholera toxin (CT) from Vibrio cholerae and heat-labile enterotoxin (LT) from enterotoxigenic Escherichia coli	103:227	The AB5 toxins cholera toxin (CT) from Vibrio cholerae and heat-labile enterotoxin (LT) from enterotoxigenic Escherichia coli are notorious for their roles in diarrheal disease, but their effect on other intestinal bacteria remains unexplored.
30918252	1	34	theme	AB5	107:109	arg1	notorious					233:241	notorious	233:241	notorious	233:241	The AB5 toxins cholera toxin (CT) from Vibrio cholerae and heat-labile enterotoxin (LT) from enterotoxigenic Escherichia coli are notorious for their roles in diarrheal disease, but their effect on other intestinal bacteria remains unexplored.
30918252	2	35	theme	LT.	446:448	arg1	receptor					427:434	the GM1 ganglioside receptor	407:434	the GM1 ganglioside receptor of CT and LT.	407:448	Another foodborne pathogen, Campylobacter jejuni, can mimic the GM1 ganglioside receptor of CT and LT. Here we demonstrate that the toxin B-subunits (CTB and LTB) inhibit C. jejuni growth by binding to GM1-mimicking lipooligosaccharides and increasing permeability of the cell membrane.
30918252	4	36	theme	mimicry	1002:1008	arg1	relevance					984:992	the relevance	980:992	the relevance of this mimicry	980:1008	Examining the chicken GI tract with immunofluorescence microscopy demonstrates that GM1 reactive structures are abundant on epithelial cells and commensal bacteria, further emphasizing the relevance of this mimicry.
30918252	4	37	theme	epithelial	919:928	arg1	cells					930:934	epithelial cells	919:934	epithelial cells	919:934	Examining the chicken GI tract with immunofluorescence microscopy demonstrates that GM1 reactive structures are abundant on epithelial cells and commensal bacteria, further emphasizing the relevance of this mimicry.
30918252	4	38	theme	reactive	883:890	arg1	structures					892:901	GM1 reactive structures	879:901	GM1 reactive structures	879:901	Examining the chicken GI tract with immunofluorescence microscopy demonstrates that GM1 reactive structures are abundant on epithelial cells and commensal bacteria, further emphasizing the relevance of this mimicry.
30918252	0	39	theme	gut	43:45	arg1	bacteria					47:54	gut bacteria	43:54	gut bacteria	43:54	Bacterial AB5 toxins inhibit the growth of gut bacteria by targeting ganglioside-like glycoconjugates.
30918252	1	40	from	coli	224:227	arg1	toxins					111:116	The AB5 toxins cholera toxin (CT)	103:135	The AB5 toxins cholera toxin (CT) from Vibrio cholerae and heat-labile enterotoxin (LT) from enterotoxigenic Escherichia coli	103:227	The AB5 toxins cholera toxin (CT) from Vibrio cholerae and heat-labile enterotoxin (LT) from enterotoxigenic Escherichia coli are notorious for their roles in diarrheal disease, but their effect on other intestinal bacteria remains unexplored.
30918252	1	40	from	coli	224:227	arg1	notorious					233:241	notorious	233:241	notorious	233:241	The AB5 toxins cholera toxin (CT) from Vibrio cholerae and heat-labile enterotoxin (LT) from enterotoxigenic Escherichia coli are notorious for their roles in diarrheal disease, but their effect on other intestinal bacteria remains unexplored.
30918252	3	41	dep	GM1	784:786	arg1	mimic					788:792	mimic	788:792	mimic	788:792	Furthermore, incubation of CTB or LTB with a C. jejuni isolate capable of altering its lipooligosaccharide structure selects for variants lacking the GM1 mimic.
30918252	5	42	theme	chickens	1023:1030	arg1	Exposure					1011:1018	Exposure	1011:1018	Exposure of chickens to CTB or LTB	1011:1044	Exposure of chickens to CTB or LTB causes shifts in the gut microbial composition, providing evidence for new toxin functions in bacterial gut competition.
30918252	5	43	theme	new	1117:1119	arg1	functions					1127:1135	new toxin functions	1117:1135	new toxin functions in bacterial gut competition	1117:1164	Exposure of chickens to CTB or LTB causes shifts in the gut microbial composition, providing evidence for new toxin functions in bacterial gut competition.
30918252	2	44	theme	CT	439:440	arg1	receptor					427:434	the GM1 ganglioside receptor	407:434	the GM1 ganglioside receptor of CT and LT.	407:448	Another foodborne pathogen, Campylobacter jejuni, can mimic the GM1 ganglioside receptor of CT and LT. Here we demonstrate that the toxin B-subunits (CTB and LTB) inhibit C. jejuni growth by binding to GM1-mimicking lipooligosaccharides and increasing permeability of the cell membrane.
30918252	3	45	theme	lipooligosaccharide	721:739	arg1	structure					741:749	its lipooligosaccharide structure	717:749	its lipooligosaccharide structure	717:749	Furthermore, incubation of CTB or LTB with a C. jejuni isolate capable of altering its lipooligosaccharide structure selects for variants lacking the GM1 mimic.
30918252	3	46	theme	LTB	668:670	arg1	incubation					647:656	incubation	647:656	incubation of CTB or LTB with a C. jejuni isolate capable of altering its lipooligosaccharide structure	647:749	Furthermore, incubation of CTB or LTB with a C. jejuni isolate capable of altering its lipooligosaccharide structure selects for variants lacking the GM1 mimic.
30918252	5	47	theme	microbial	1071:1079	arg1	composition					1081:1091	the gut microbial composition	1063:1091	the gut microbial composition	1063:1091	Exposure of chickens to CTB or LTB causes shifts in the gut microbial composition, providing evidence for new toxin functions in bacterial gut competition.
30918252	1	48	theme	cholera	118:124	arg1	toxin					126:130	cholera toxin	118:130	The AB5 toxins cholera toxin (CT) from Vibrio cholerae and heat-labile enterotoxin (LT) from enterotoxigenic Escherichia coli	103:227	The AB5 toxins cholera toxin (CT) from Vibrio cholerae and heat-labile enterotoxin (LT) from enterotoxigenic Escherichia coli are notorious for their roles in diarrheal disease, but their effect on other intestinal bacteria remains unexplored.
30918252	1	48	theme	cholera	118:124	arg1	CT					133:134	CT	133:134	CT	133:134	The AB5 toxins cholera toxin (CT) from Vibrio cholerae and heat-labile enterotoxin (LT) from enterotoxigenic Escherichia coli are notorious for their roles in diarrheal disease, but their effect on other intestinal bacteria remains unexplored.
30918252	5	49	theme	gut	1150:1152	arg1	competition					1154:1164	bacterial gut competition	1140:1164	bacterial gut competition	1140:1164	Exposure of chickens to CTB or LTB causes shifts in the gut microbial composition, providing evidence for new toxin functions in bacterial gut competition.
30918252	2	50	theme	jejuni	521:526	arg1	growth					528:533	C. jejuni growth	518:533	C. jejuni growth	518:533	Another foodborne pathogen, Campylobacter jejuni, can mimic the GM1 ganglioside receptor of CT and LT. Here we demonstrate that the toxin B-subunits (CTB and LTB) inhibit C. jejuni growth by binding to GM1-mimicking lipooligosaccharides and increasing permeability of the cell membrane.
30918252	2	51	theme	C.	518:519	arg1	growth					528:533	C. jejuni growth	518:533	C. jejuni growth	518:533	Another foodborne pathogen, Campylobacter jejuni, can mimic the GM1 ganglioside receptor of CT and LT. Here we demonstrate that the toxin B-subunits (CTB and LTB) inhibit C. jejuni growth by binding to GM1-mimicking lipooligosaccharides and increasing permeability of the cell membrane.
30918252	1	52	from	enterotoxin	174:184	arg1	toxins					111:116	The AB5 toxins cholera toxin (CT)	103:135	The AB5 toxins cholera toxin (CT) from Vibrio cholerae and heat-labile enterotoxin (LT) from enterotoxigenic Escherichia coli	103:227	The AB5 toxins cholera toxin (CT) from Vibrio cholerae and heat-labile enterotoxin (LT) from enterotoxigenic Escherichia coli are notorious for their roles in diarrheal disease, but their effect on other intestinal bacteria remains unexplored.
30918252	1	52	from	enterotoxin	174:184	arg1	notorious					233:241	notorious	233:241	notorious	233:241	The AB5 toxins cholera toxin (CT) from Vibrio cholerae and heat-labile enterotoxin (LT) from enterotoxigenic Escherichia coli are notorious for their roles in diarrheal disease, but their effect on other intestinal bacteria remains unexplored.
30918252	2	53	theme	foodborne	355:363	arg1	pathogen					365:372	Another foodborne pathogen	347:372	Another foodborne pathogen	347:372	Another foodborne pathogen, Campylobacter jejuni, can mimic the GM1 ganglioside receptor of CT and LT. Here we demonstrate that the toxin B-subunits (CTB and LTB) inhibit C. jejuni growth by binding to GM1-mimicking lipooligosaccharides and increasing permeability of the cell membrane.
30918252	2	53	theme	foodborne	355:363	arg1	jejuni					389:394	Campylobacter jejuni	375:394	Campylobacter jejuni	375:394	Another foodborne pathogen, Campylobacter jejuni, can mimic the GM1 ganglioside receptor of CT and LT. Here we demonstrate that the toxin B-subunits (CTB and LTB) inhibit C. jejuni growth by binding to GM1-mimicking lipooligosaccharides and increasing permeability of the cell membrane.
30918252	0	54	theme	bacteria	47:54	arg1	growth					33:38	the growth	29:38	the growth of gut bacteria	29:54	Bacterial AB5 toxins inhibit the growth of gut bacteria by targeting ganglioside-like glycoconjugates.
30918252	1	55	theme	other	301:305	arg1	bacteria					318:325	other intestinal bacteria	301:325	other intestinal bacteria	301:325	The AB5 toxins cholera toxin (CT) from Vibrio cholerae and heat-labile enterotoxin (LT) from enterotoxigenic Escherichia coli are notorious for their roles in diarrheal disease, but their effect on other intestinal bacteria remains unexplored.
30918252	1	56	from	cholerae	149:156	arg1	toxins					111:116	The AB5 toxins cholera toxin (CT)	103:135	The AB5 toxins cholera toxin (CT) from Vibrio cholerae and heat-labile enterotoxin (LT) from enterotoxigenic Escherichia coli	103:227	The AB5 toxins cholera toxin (CT) from Vibrio cholerae and heat-labile enterotoxin (LT) from enterotoxigenic Escherichia coli are notorious for their roles in diarrheal disease, but their effect on other intestinal bacteria remains unexplored.
30918252	1	56	from	cholerae	149:156	arg1	notorious					233:241	notorious	233:241	notorious	233:241	The AB5 toxins cholera toxin (CT) from Vibrio cholerae and heat-labile enterotoxin (LT) from enterotoxigenic Escherichia coli are notorious for their roles in diarrheal disease, but their effect on other intestinal bacteria remains unexplored.
30605074	11	0	theme	=CCTCC AA	1310:1318	arg1	105902T					1345:1351	=CCTCC AA 2017044T=KCTC 49031T=DSM 105902T	1310:1351	=CCTCC AA 2017044T=KCTC 49031T=DSM 105902T	1310:1351	The type strain is YIM PH21725T (=CCTCC AA 2017044T=KCTC 49031T=DSM 105902T).
30605074	11	0	theme	=CCTCC AA	1310:1318	arg1	PH21725T					1300:1307	YIM PH21725T	1296:1307	YIM PH21725T (=CCTCC AA 2017044T=KCTC 49031T=DSM 105902T)	1296:1352	The type strain is YIM PH21725T (=CCTCC AA 2017044T=KCTC 49031T=DSM 105902T).
30605074	2	1	theme	designated	116:125	arg1	PH21725T					138:145	designated strain YIM PH21725T	116:145	designated strain YIM PH21725T	116:145	A novel Gram-positive bacterium, designated strain YIM PH21725T, was isolated from a sample of rhizospheric soil of Panaxnotoginseng cultivated in Anning, Yunnan.
30605074	2	1	theme	designated	116:125	arg1	bacterium					105:113	A novel Gram-positive bacterium	83:113	A novel Gram-positive bacterium	83:113	A novel Gram-positive bacterium, designated strain YIM PH21725T, was isolated from a sample of rhizospheric soil of Panaxnotoginseng cultivated in Anning, Yunnan.
30605074	4	2	theme	main	328:331	arg1	acids					339:343	The main fatty acids	324:343	The main fatty acids identified	324:354	The main fatty acids identified were C17 : 0, iso-C15 : 0, iso-C16 : 0 and C16 : 0.
30605074	4	2	theme	main	328:331	arg1	C17 					361:364	C17 	361:364	C17 	361:364	The main fatty acids identified were C17 : 0, iso-C15 : 0, iso-C16 : 0 and C16 : 0.
30605074	6	3	theme	unidentified	612:623	arg1	structure					625:633	an unidentified structure	609:633	an unidentified structure containing glucosamine	609:656	The polar lipids included phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol mannoside, phosphatidylinositol, phospholipids and phospholipids of an unidentified structure containing glucosamine.
30605074	9	4	theme	name	1229:1232	arg1	sp					1255:1256	the name Amycolatopsispanacis sp	1225:1256	the name Amycolatopsispanacis sp	1225:1256	The phenotypic, chemotaxonomic, phylogenetic and digital DNA-DNA hybridization results clearly indicated that strain YIM PH21725T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsispanacis sp.
30605074	9	5	theme	Amycolatopsispanacis	1234:1253	arg1	sp					1255:1256	the name Amycolatopsispanacis sp	1225:1256	the name Amycolatopsispanacis sp	1225:1256	The phenotypic, chemotaxonomic, phylogenetic and digital DNA-DNA hybridization results clearly indicated that strain YIM PH21725T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsispanacis sp.
30605074	9	6	theme	genus	1194:1198	arg1	Amycolatopsis					1200:1212	the genus Amycolatopsis	1190:1212	the genus Amycolatopsis	1190:1212	The phenotypic, chemotaxonomic, phylogenetic and digital DNA-DNA hybridization results clearly indicated that strain YIM PH21725T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsispanacis sp.
30605074	8	7	theme	Amycolatopsis	983:995	arg1	16989T					1013:1018	Amycolatopsis ultiminotia JCM 16989T	983:1018	Amycolatopsis ultiminotia JCM 16989T (96.8 %)	983:1027	On the basis of its 16S rRNA gene sequence, strain YIM PH21725T should belong to the genus Amycolatopsis, and was closely related to Amycolatopsis sulphurea DSM 46092T (98.57 %), Amycolatopsis jejuensis JCM13280T (97.27 %), Amycolatopsis jiangsuensis KCTC 19885T (96.88 %) and Amycolatopsis ultiminotia JCM 16989T (96.8 %).
30605074	8	7	theme	Amycolatopsis	983:995	arg1	%					1026:1026	96.8 %	1021:1026	96.8 %	1021:1026	On the basis of its 16S rRNA gene sequence, strain YIM PH21725T should belong to the genus Amycolatopsis, and was closely related to Amycolatopsis sulphurea DSM 46092T (98.57 %), Amycolatopsis jejuensis JCM13280T (97.27 %), Amycolatopsis jiangsuensis KCTC 19885T (96.88 %) and Amycolatopsis ultiminotia JCM 16989T (96.8 %).
30605074	9	8	theme	strain	1140:1145	arg1	PH21725T					1151:1158	strain YIM PH21725T	1140:1158	strain YIM PH21725T	1140:1158	The phenotypic, chemotaxonomic, phylogenetic and digital DNA-DNA hybridization results clearly indicated that strain YIM PH21725T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsispanacis sp.
30605074	1	9	theme	Panax	46:50	arg1	soil					77:80	Panax notoginseng rhizospheric soil	46:80	Panax notoginseng rhizospheric soil	46:80	nov., isolated from Panax notoginseng rhizospheric soil.
30605074	8	10	theme	jiangsuensis	944:955	arg1	19885T					962:967	Amycolatopsis jiangsuensis KCTC 19885T	930:967	Amycolatopsis jiangsuensis KCTC 19885T (96.88 %)	930:977	On the basis of its 16S rRNA gene sequence, strain YIM PH21725T should belong to the genus Amycolatopsis, and was closely related to Amycolatopsis sulphurea DSM 46092T (98.57 %), Amycolatopsis jejuensis JCM13280T (97.27 %), Amycolatopsis jiangsuensis KCTC 19885T (96.88 %) and Amycolatopsis ultiminotia JCM 16989T (96.8 %).
30605074	8	10	theme	jiangsuensis	944:955	arg1	%					976:976	96.88 %	970:976	96.88 %	970:976	On the basis of its 16S rRNA gene sequence, strain YIM PH21725T should belong to the genus Amycolatopsis, and was closely related to Amycolatopsis sulphurea DSM 46092T (98.57 %), Amycolatopsis jejuensis JCM13280T (97.27 %), Amycolatopsis jiangsuensis KCTC 19885T (96.88 %) and Amycolatopsis ultiminotia JCM 16989T (96.8 %).
30605074	1	11	theme	notoginseng	52:62	arg1	soil					77:80	Panax notoginseng rhizospheric soil	46:80	Panax notoginseng rhizospheric soil	46:80	nov., isolated from Panax notoginseng rhizospheric soil.
30605074	8	12	theme	Amycolatopsis	839:851	arg1	46092T					867:872	Amycolatopsis sulphurea DSM 46092T	839:872	Amycolatopsis sulphurea DSM 46092T (98.57 %)	839:882	On the basis of its 16S rRNA gene sequence, strain YIM PH21725T should belong to the genus Amycolatopsis, and was closely related to Amycolatopsis sulphurea DSM 46092T (98.57 %), Amycolatopsis jejuensis JCM13280T (97.27 %), Amycolatopsis jiangsuensis KCTC 19885T (96.88 %) and Amycolatopsis ultiminotia JCM 16989T (96.8 %).
30605074	8	12	theme	Amycolatopsis	839:851	arg1	%					881:881	98.57 %	875:881	98.57 %	875:881	On the basis of its 16S rRNA gene sequence, strain YIM PH21725T should belong to the genus Amycolatopsis, and was closely related to Amycolatopsis sulphurea DSM 46092T (98.57 %), Amycolatopsis jejuensis JCM13280T (97.27 %), Amycolatopsis jiangsuensis KCTC 19885T (96.88 %) and Amycolatopsis ultiminotia JCM 16989T (96.8 %).
30605074	3	13	from	acid	287:290	arg1	peptidoglycan					309:321	the cell-wall peptidoglycan	295:321	the cell-wall peptidoglycan	295:321	The strain contained meso-diaminopimelic acid in the cell-wall peptidoglycan.
30605074	6	14	theme	polar	448:452	arg1	lipids					454:459	The polar lipids	444:459	The polar lipids	444:459	The polar lipids included phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol mannoside, phosphatidylinositol, phospholipids and phospholipids of an unidentified structure containing glucosamine.
30605074	8	15	theme	JCM	1009:1011	arg1	16989T					1013:1018	Amycolatopsis ultiminotia JCM 16989T	983:1018	Amycolatopsis ultiminotia JCM 16989T (96.8 %)	983:1027	On the basis of its 16S rRNA gene sequence, strain YIM PH21725T should belong to the genus Amycolatopsis, and was closely related to Amycolatopsis sulphurea DSM 46092T (98.57 %), Amycolatopsis jejuensis JCM13280T (97.27 %), Amycolatopsis jiangsuensis KCTC 19885T (96.88 %) and Amycolatopsis ultiminotia JCM 16989T (96.8 %).
30605074	8	15	theme	JCM	1009:1011	arg1	%					1026:1026	96.8 %	1021:1026	96.8 %	1021:1026	On the basis of its 16S rRNA gene sequence, strain YIM PH21725T should belong to the genus Amycolatopsis, and was closely related to Amycolatopsis sulphurea DSM 46092T (98.57 %), Amycolatopsis jejuensis JCM13280T (97.27 %), Amycolatopsis jiangsuensis KCTC 19885T (96.88 %) and Amycolatopsis ultiminotia JCM 16989T (96.8 %).
30605074	8	16	theme	Amycolatopsis	930:942	arg1	19885T					962:967	Amycolatopsis jiangsuensis KCTC 19885T	930:967	Amycolatopsis jiangsuensis KCTC 19885T (96.88 %)	930:977	On the basis of its 16S rRNA gene sequence, strain YIM PH21725T should belong to the genus Amycolatopsis, and was closely related to Amycolatopsis sulphurea DSM 46092T (98.57 %), Amycolatopsis jejuensis JCM13280T (97.27 %), Amycolatopsis jiangsuensis KCTC 19885T (96.88 %) and Amycolatopsis ultiminotia JCM 16989T (96.8 %).
30605074	8	16	theme	Amycolatopsis	930:942	arg1	%					976:976	96.88 %	970:976	96.88 %	970:976	On the basis of its 16S rRNA gene sequence, strain YIM PH21725T should belong to the genus Amycolatopsis, and was closely related to Amycolatopsis sulphurea DSM 46092T (98.57 %), Amycolatopsis jejuensis JCM13280T (97.27 %), Amycolatopsis jiangsuensis KCTC 19885T (96.88 %) and Amycolatopsis ultiminotia JCM 16989T (96.8 %).
30605074	1	17	theme	rhizospheric	64:75	arg1	soil					77:80	Panax notoginseng rhizospheric soil	46:80	Panax notoginseng rhizospheric soil	46:80	nov., isolated from Panax notoginseng rhizospheric soil.
30605074	11	18	theme	YIM	1296:1298	arg1	strain					1286:1291	The type strain	1277:1291	The type strain	1277:1291	The type strain is YIM PH21725T (=CCTCC AA 2017044T=KCTC 49031T=DSM 105902T).
30605074	11	18	theme	YIM	1296:1298	arg1	PH21725T					1300:1307	YIM PH21725T	1296:1307	YIM PH21725T (=CCTCC AA 2017044T=KCTC 49031T=DSM 105902T)	1296:1352	The type strain is YIM PH21725T (=CCTCC AA 2017044T=KCTC 49031T=DSM 105902T).
30605074	11	18	theme	YIM	1296:1298	arg1	105902T					1345:1351	=CCTCC AA 2017044T=KCTC 49031T=DSM 105902T	1310:1351	=CCTCC AA 2017044T=KCTC 49031T=DSM 105902T	1310:1351	The type strain is YIM PH21725T (=CCTCC AA 2017044T=KCTC 49031T=DSM 105902T).
30605074	8	19	theme	ultiminotia	997:1007	arg1	16989T					1013:1018	Amycolatopsis ultiminotia JCM 16989T	983:1018	Amycolatopsis ultiminotia JCM 16989T (96.8 %)	983:1027	On the basis of its 16S rRNA gene sequence, strain YIM PH21725T should belong to the genus Amycolatopsis, and was closely related to Amycolatopsis sulphurea DSM 46092T (98.57 %), Amycolatopsis jejuensis JCM13280T (97.27 %), Amycolatopsis jiangsuensis KCTC 19885T (96.88 %) and Amycolatopsis ultiminotia JCM 16989T (96.8 %).
30605074	8	19	theme	ultiminotia	997:1007	arg1	%					1026:1026	96.8 %	1021:1026	96.8 %	1021:1026	On the basis of its 16S rRNA gene sequence, strain YIM PH21725T should belong to the genus Amycolatopsis, and was closely related to Amycolatopsis sulphurea DSM 46092T (98.57 %), Amycolatopsis jejuensis JCM13280T (97.27 %), Amycolatopsis jiangsuensis KCTC 19885T (96.88 %) and Amycolatopsis ultiminotia JCM 16989T (96.8 %).
30605074	8	20	theme	DSM	863:865	arg1	46092T					867:872	Amycolatopsis sulphurea DSM 46092T	839:872	Amycolatopsis sulphurea DSM 46092T (98.57 %)	839:882	On the basis of its 16S rRNA gene sequence, strain YIM PH21725T should belong to the genus Amycolatopsis, and was closely related to Amycolatopsis sulphurea DSM 46092T (98.57 %), Amycolatopsis jejuensis JCM13280T (97.27 %), Amycolatopsis jiangsuensis KCTC 19885T (96.88 %) and Amycolatopsis ultiminotia JCM 16989T (96.8 %).
30605074	8	20	theme	DSM	863:865	arg1	%					881:881	98.57 %	875:881	98.57 %	875:881	On the basis of its 16S rRNA gene sequence, strain YIM PH21725T should belong to the genus Amycolatopsis, and was closely related to Amycolatopsis sulphurea DSM 46092T (98.57 %), Amycolatopsis jejuensis JCM13280T (97.27 %), Amycolatopsis jiangsuensis KCTC 19885T (96.88 %) and Amycolatopsis ultiminotia JCM 16989T (96.8 %).
30605074	8	21	theme	gene	735:738	arg1	sequence					740:747	its 16S rRNA gene sequence	722:747	its 16S rRNA gene sequence	722:747	On the basis of its 16S rRNA gene sequence, strain YIM PH21725T should belong to the genus Amycolatopsis, and was closely related to Amycolatopsis sulphurea DSM 46092T (98.57 %), Amycolatopsis jejuensis JCM13280T (97.27 %), Amycolatopsis jiangsuensis KCTC 19885T (96.88 %) and Amycolatopsis ultiminotia JCM 16989T (96.8 %).
30605074	0	22	theme	panacis	14:20	arg1	sp					22:23	Amycolatopsis panacis sp	0:23	Amycolatopsis panacis sp.	0:24	Amycolatopsis panacis sp.
30605074	2	23	theme	YIM	134:136	arg1	PH21725T					138:145	designated strain YIM PH21725T	116:145	designated strain YIM PH21725T	116:145	A novel Gram-positive bacterium, designated strain YIM PH21725T, was isolated from a sample of rhizospheric soil of Panaxnotoginseng cultivated in Anning, Yunnan.
30605074	2	23	theme	YIM	134:136	arg1	bacterium					105:113	A novel Gram-positive bacterium	83:113	A novel Gram-positive bacterium	83:113	A novel Gram-positive bacterium, designated strain YIM PH21725T, was isolated from a sample of rhizospheric soil of Panaxnotoginseng cultivated in Anning, Yunnan.
30605074	8	24	theme	KCTC	957:960	arg1	19885T					962:967	Amycolatopsis jiangsuensis KCTC 19885T	930:967	Amycolatopsis jiangsuensis KCTC 19885T (96.88 %)	930:977	On the basis of its 16S rRNA gene sequence, strain YIM PH21725T should belong to the genus Amycolatopsis, and was closely related to Amycolatopsis sulphurea DSM 46092T (98.57 %), Amycolatopsis jejuensis JCM13280T (97.27 %), Amycolatopsis jiangsuensis KCTC 19885T (96.88 %) and Amycolatopsis ultiminotia JCM 16989T (96.8 %).
30605074	8	24	theme	KCTC	957:960	arg1	%					976:976	96.88 %	970:976	96.88 %	970:976	On the basis of its 16S rRNA gene sequence, strain YIM PH21725T should belong to the genus Amycolatopsis, and was closely related to Amycolatopsis sulphurea DSM 46092T (98.57 %), Amycolatopsis jejuensis JCM13280T (97.27 %), Amycolatopsis jiangsuensis KCTC 19885T (96.88 %) and Amycolatopsis ultiminotia JCM 16989T (96.8 %).
30605074	8	25	theme	sulphurea	853:861	arg1	46092T					867:872	Amycolatopsis sulphurea DSM 46092T	839:872	Amycolatopsis sulphurea DSM 46092T (98.57 %)	839:882	On the basis of its 16S rRNA gene sequence, strain YIM PH21725T should belong to the genus Amycolatopsis, and was closely related to Amycolatopsis sulphurea DSM 46092T (98.57 %), Amycolatopsis jejuensis JCM13280T (97.27 %), Amycolatopsis jiangsuensis KCTC 19885T (96.88 %) and Amycolatopsis ultiminotia JCM 16989T (96.8 %).
30605074	8	25	theme	sulphurea	853:861	arg1	%					881:881	98.57 %	875:881	98.57 %	875:881	On the basis of its 16S rRNA gene sequence, strain YIM PH21725T should belong to the genus Amycolatopsis, and was closely related to Amycolatopsis sulphurea DSM 46092T (98.57 %), Amycolatopsis jejuensis JCM13280T (97.27 %), Amycolatopsis jiangsuensis KCTC 19885T (96.88 %) and Amycolatopsis ultiminotia JCM 16989T (96.8 %).
30605074	0	26	theme	Amycolatopsis	0:12	arg1	sp					22:23	Amycolatopsis panacis sp	0:23	Amycolatopsis panacis sp.	0:24	Amycolatopsis panacis sp.
30605074	9	27	theme	novel	1173:1177	arg1	species					1179:1185	a novel species	1171:1185	a novel species	1171:1185	The phenotypic, chemotaxonomic, phylogenetic and digital DNA-DNA hybridization results clearly indicated that strain YIM PH21725T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsispanacis sp.
30605074	9	28	theme	Amycolatopsis	1200:1212	arg1	species					1179:1185	a novel species	1171:1185	a novel species	1171:1185	The phenotypic, chemotaxonomic, phylogenetic and digital DNA-DNA hybridization results clearly indicated that strain YIM PH21725T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsispanacis sp.
30605074	4	29	dep	C17 	361:364	arg1	 0					392:393	 0	392:393	 0	392:393	The main fatty acids identified were C17 : 0, iso-C15 : 0, iso-C16 : 0 and C16 : 0.
30605074	4	29	dep	C17 	361:364	arg1	 0					366:367	 0	366:367	 0	366:367	The main fatty acids identified were C17 : 0, iso-C15 : 0, iso-C16 : 0 and C16 : 0.
30605074	3	30	theme	meso-diaminopimelic	267:285	arg1	acid					287:290	meso-diaminopimelic acid	267:290	meso-diaminopimelic acid in the cell-wall peptidoglycan	267:321	The strain contained meso-diaminopimelic acid in the cell-wall peptidoglycan.
30605074	9	31	theme	YIM	1147:1149	arg1	PH21725T					1151:1158	strain YIM PH21725T	1140:1158	strain YIM PH21725T	1140:1158	The phenotypic, chemotaxonomic, phylogenetic and digital DNA-DNA hybridization results clearly indicated that strain YIM PH21725T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsispanacis sp.
30605074	9	32	theme	phenotypic	1034:1043	arg1	results					1109:1115	The phenotypic, chemotaxonomic, phylogenetic and digital DNA-DNA hybridization results	1030:1115	The phenotypic, chemotaxonomic, phylogenetic and digital DNA-DNA hybridization results	1030:1115	The phenotypic, chemotaxonomic, phylogenetic and digital DNA-DNA hybridization results clearly indicated that strain YIM PH21725T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsispanacis sp.
30605074	9	33	theme	hybridization	1095:1107	arg1	results					1109:1115	The phenotypic, chemotaxonomic, phylogenetic and digital DNA-DNA hybridization results	1030:1115	The phenotypic, chemotaxonomic, phylogenetic and digital DNA-DNA hybridization results	1030:1115	The phenotypic, chemotaxonomic, phylogenetic and digital DNA-DNA hybridization results clearly indicated that strain YIM PH21725T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsispanacis sp.
30605074	3	34	contain	contained	257:265	arg1	strain					250:255	The strain	246:255	The strain	246:255	The strain contained meso-diaminopimelic acid in the cell-wall peptidoglycan.
30605074	3	34	contain	contained	257:265	arg2	acid					287:290	meso-diaminopimelic acid	267:290	meso-diaminopimelic acid in the cell-wall peptidoglycan	267:321	The strain contained meso-diaminopimelic acid in the cell-wall peptidoglycan.
30605074	9	35	theme	chemotaxonomic	1046:1059	arg1	results					1109:1115	The phenotypic, chemotaxonomic, phylogenetic and digital DNA-DNA hybridization results	1030:1115	The phenotypic, chemotaxonomic, phylogenetic and digital DNA-DNA hybridization results	1030:1115	The phenotypic, chemotaxonomic, phylogenetic and digital DNA-DNA hybridization results clearly indicated that strain YIM PH21725T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsispanacis sp.
30605074	2	36	attach	isolated	152:159	arg2	bacterium					105:113	A novel Gram-positive bacterium	83:113	A novel Gram-positive bacterium	83:113	A novel Gram-positive bacterium, designated strain YIM PH21725T, was isolated from a sample of rhizospheric soil of Panaxnotoginseng cultivated in Anning, Yunnan.
30605074	2	36	attach	isolated	152:159	arg2	PH21725T					138:145	designated strain YIM PH21725T	116:145	designated strain YIM PH21725T	116:145	A novel Gram-positive bacterium, designated strain YIM PH21725T, was isolated from a sample of rhizospheric soil of Panaxnotoginseng cultivated in Anning, Yunnan.
30605074	2	36	attach	isolated	152:159	arg1	sample					168:173	a sample	166:173	a sample of rhizospheric soil of Panaxnotoginseng cultivated in Anning, Yunnan	166:243	A novel Gram-positive bacterium, designated strain YIM PH21725T, was isolated from a sample of rhizospheric soil of Panaxnotoginseng cultivated in Anning, Yunnan.
30605074	2	37	theme	Gram-positive	91:103	arg1	PH21725T					138:145	designated strain YIM PH21725T	116:145	designated strain YIM PH21725T	116:145	A novel Gram-positive bacterium, designated strain YIM PH21725T, was isolated from a sample of rhizospheric soil of Panaxnotoginseng cultivated in Anning, Yunnan.
30605074	2	37	theme	Gram-positive	91:103	arg1	bacterium					105:113	A novel Gram-positive bacterium	83:113	A novel Gram-positive bacterium	83:113	A novel Gram-positive bacterium, designated strain YIM PH21725T, was isolated from a sample of rhizospheric soil of Panaxnotoginseng cultivated in Anning, Yunnan.
30605074	2	38	theme	novel	85:89	arg1	PH21725T					138:145	designated strain YIM PH21725T	116:145	designated strain YIM PH21725T	116:145	A novel Gram-positive bacterium, designated strain YIM PH21725T, was isolated from a sample of rhizospheric soil of Panaxnotoginseng cultivated in Anning, Yunnan.
30605074	2	38	theme	novel	85:89	arg1	bacterium					105:113	A novel Gram-positive bacterium	83:113	A novel Gram-positive bacterium	83:113	A novel Gram-positive bacterium, designated strain YIM PH21725T, was isolated from a sample of rhizospheric soil of Panaxnotoginseng cultivated in Anning, Yunnan.
30605074	3	39	theme	cell-wall	299:307	arg1	peptidoglycan					309:321	the cell-wall peptidoglycan	295:321	the cell-wall peptidoglycan	295:321	The strain contained meso-diaminopimelic acid in the cell-wall peptidoglycan.
30605074	7	40	theme	69.43 mol	694:702	arg1	G+C content					663:673	The G+C content	659:673	The G+C content of genomic DNA	659:688	The G+C content of genomic DNA was 69.43 mol%.
30605074	7	40	theme	69.43 mol	694:702	arg1	%					703:703	69.43 mol%	694:703	69.43 mol%	694:703	The G+C content of genomic DNA was 69.43 mol%.
30605074	9	41	theme	phylogenetic	1062:1073	arg1	results					1109:1115	The phenotypic, chemotaxonomic, phylogenetic and digital DNA-DNA hybridization results	1030:1115	The phenotypic, chemotaxonomic, phylogenetic and digital DNA-DNA hybridization results	1030:1115	The phenotypic, chemotaxonomic, phylogenetic and digital DNA-DNA hybridization results clearly indicated that strain YIM PH21725T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsispanacis sp.
30605074	6	42	theme	phosphatidylinositol	520:539	arg1	mannoside					541:549	phosphatidylinositol mannoside	520:549	phosphatidylinositol mannoside	520:549	The polar lipids included phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol mannoside, phosphatidylinositol, phospholipids and phospholipids of an unidentified structure containing glucosamine.
30605074	6	42	theme	phosphatidylinositol	520:539	arg1	phosphatidylethanolamine					470:493	phosphatidylethanolamine	470:493	phosphatidylethanolamine	470:493	The polar lipids included phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol mannoside, phosphatidylinositol, phospholipids and phospholipids of an unidentified structure containing glucosamine.
30605074	8	43	theme	16S	726:728	arg1	sequence					740:747	its 16S rRNA gene sequence	722:747	its 16S rRNA gene sequence	722:747	On the basis of its 16S rRNA gene sequence, strain YIM PH21725T should belong to the genus Amycolatopsis, and was closely related to Amycolatopsis sulphurea DSM 46092T (98.57 %), Amycolatopsis jejuensis JCM13280T (97.27 %), Amycolatopsis jiangsuensis KCTC 19885T (96.88 %) and Amycolatopsis ultiminotia JCM 16989T (96.8 %).
30605074	6	44	contain	containing	635:644	arg2	glucosamine					646:656	glucosamine	646:656	glucosamine	646:656	The polar lipids included phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol mannoside, phosphatidylinositol, phospholipids and phospholipids of an unidentified structure containing glucosamine.
30605074	6	44	contain	containing	635:644	arg1	structure					625:633	an unidentified structure	609:633	an unidentified structure containing glucosamine	609:656	The polar lipids included phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol mannoside, phosphatidylinositol, phospholipids and phospholipids of an unidentified structure containing glucosamine.
30605074	7	45	theme	genomic	678:684	arg1	DNA					686:688	genomic DNA	678:688	genomic DNA	678:688	The G+C content of genomic DNA was 69.43 mol%.
30605074	2	46	theme	Panaxnotoginseng	199:214	arg1	soil					191:194	rhizospheric soil	178:194	rhizospheric soil of Panaxnotoginseng cultivated in Anning, Yunnan	178:243	A novel Gram-positive bacterium, designated strain YIM PH21725T, was isolated from a sample of rhizospheric soil of Panaxnotoginseng cultivated in Anning, Yunnan.
30605074	8	47	theme	jejuensis	899:907	arg1	JCM13280T					909:917	Amycolatopsis jejuensis JCM13280T	885:917	Amycolatopsis jejuensis JCM13280T (97.27 %)	885:927	On the basis of its 16S rRNA gene sequence, strain YIM PH21725T should belong to the genus Amycolatopsis, and was closely related to Amycolatopsis sulphurea DSM 46092T (98.57 %), Amycolatopsis jejuensis JCM13280T (97.27 %), Amycolatopsis jiangsuensis KCTC 19885T (96.88 %) and Amycolatopsis ultiminotia JCM 16989T (96.8 %).
30605074	8	47	theme	jejuensis	899:907	arg1	%					926:926	97.27 %	920:926	97.27 %	920:926	On the basis of its 16S rRNA gene sequence, strain YIM PH21725T should belong to the genus Amycolatopsis, and was closely related to Amycolatopsis sulphurea DSM 46092T (98.57 %), Amycolatopsis jejuensis JCM13280T (97.27 %), Amycolatopsis jiangsuensis KCTC 19885T (96.88 %) and Amycolatopsis ultiminotia JCM 16989T (96.8 %).
30605074	8	48	theme	genus	791:795	arg1	Amycolatopsis					797:809	the genus Amycolatopsis	787:809	the genus Amycolatopsis	787:809	On the basis of its 16S rRNA gene sequence, strain YIM PH21725T should belong to the genus Amycolatopsis, and was closely related to Amycolatopsis sulphurea DSM 46092T (98.57 %), Amycolatopsis jejuensis JCM13280T (97.27 %), Amycolatopsis jiangsuensis KCTC 19885T (96.88 %) and Amycolatopsis ultiminotia JCM 16989T (96.8 %).
30605074	8	49	theme	rRNA	730:733	arg1	sequence					740:747	its 16S rRNA gene sequence	722:747	its 16S rRNA gene sequence	722:747	On the basis of its 16S rRNA gene sequence, strain YIM PH21725T should belong to the genus Amycolatopsis, and was closely related to Amycolatopsis sulphurea DSM 46092T (98.57 %), Amycolatopsis jejuensis JCM13280T (97.27 %), Amycolatopsis jiangsuensis KCTC 19885T (96.88 %) and Amycolatopsis ultiminotia JCM 16989T (96.8 %).
30605074	2	50	theme	soil	191:194	arg1	sample					168:173	a sample	166:173	a sample of rhizospheric soil of Panaxnotoginseng cultivated in Anning, Yunnan	166:243	A novel Gram-positive bacterium, designated strain YIM PH21725T, was isolated from a sample of rhizospheric soil of Panaxnotoginseng cultivated in Anning, Yunnan.
30605074	7	51	theme	DNA	686:688	arg1	G+C content					663:673	The G+C content	659:673	The G+C content of genomic DNA	659:688	The G+C content of genomic DNA was 69.43 mol%.
30605074	7	51	theme	DNA	686:688	arg1	%					703:703	69.43 mol%	694:703	69.43 mol%	694:703	The G+C content of genomic DNA was 69.43 mol%.
30605074	11	52	theme	2017044T=KCTC	1320:1332	arg1	105902T					1345:1351	=CCTCC AA 2017044T=KCTC 49031T=DSM 105902T	1310:1351	=CCTCC AA 2017044T=KCTC 49031T=DSM 105902T	1310:1351	The type strain is YIM PH21725T (=CCTCC AA 2017044T=KCTC 49031T=DSM 105902T).
30605074	11	52	theme	2017044T=KCTC	1320:1332	arg1	PH21725T					1300:1307	YIM PH21725T	1296:1307	YIM PH21725T (=CCTCC AA 2017044T=KCTC 49031T=DSM 105902T)	1296:1352	The type strain is YIM PH21725T (=CCTCC AA 2017044T=KCTC 49031T=DSM 105902T).
30605074	9	53	theme	digital	1079:1085	arg1	results					1109:1115	The phenotypic, chemotaxonomic, phylogenetic and digital DNA-DNA hybridization results	1030:1115	The phenotypic, chemotaxonomic, phylogenetic and digital DNA-DNA hybridization results	1030:1115	The phenotypic, chemotaxonomic, phylogenetic and digital DNA-DNA hybridization results clearly indicated that strain YIM PH21725T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsispanacis sp.
30605074	8	54	theme	Amycolatopsis	885:897	arg1	JCM13280T					909:917	Amycolatopsis jejuensis JCM13280T	885:917	Amycolatopsis jejuensis JCM13280T (97.27 %)	885:927	On the basis of its 16S rRNA gene sequence, strain YIM PH21725T should belong to the genus Amycolatopsis, and was closely related to Amycolatopsis sulphurea DSM 46092T (98.57 %), Amycolatopsis jejuensis JCM13280T (97.27 %), Amycolatopsis jiangsuensis KCTC 19885T (96.88 %) and Amycolatopsis ultiminotia JCM 16989T (96.8 %).
30605074	8	54	theme	Amycolatopsis	885:897	arg1	%					926:926	97.27 %	920:926	97.27 %	920:926	On the basis of its 16S rRNA gene sequence, strain YIM PH21725T should belong to the genus Amycolatopsis, and was closely related to Amycolatopsis sulphurea DSM 46092T (98.57 %), Amycolatopsis jejuensis JCM13280T (97.27 %), Amycolatopsis jiangsuensis KCTC 19885T (96.88 %) and Amycolatopsis ultiminotia JCM 16989T (96.8 %).
30605074	2	55	theme	rhizospheric	178:189	arg1	soil					191:194	rhizospheric soil	178:194	rhizospheric soil of Panaxnotoginseng cultivated in Anning, Yunnan	178:243	A novel Gram-positive bacterium, designated strain YIM PH21725T, was isolated from a sample of rhizospheric soil of Panaxnotoginseng cultivated in Anning, Yunnan.
30605074	9	56	theme	DNA-DNA	1087:1093	arg1	hybridization					1095:1107	DNA-DNA hybridization	1087:1107	The phenotypic, chemotaxonomic, phylogenetic and digital DNA-DNA hybridization results	1030:1115	The phenotypic, chemotaxonomic, phylogenetic and digital DNA-DNA hybridization results clearly indicated that strain YIM PH21725T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsispanacis sp.
30605074	4	57	dep	 0	366:367	arg1	 0					379:380	 0	379:380	 0	379:380	The main fatty acids identified were C17 : 0, iso-C15 : 0, iso-C16 : 0 and C16 : 0.
30605074	8	58	theme	sequence	740:747	arg1	basis					713:717	the basis	709:717	the basis of its 16S rRNA gene sequence	709:747	On the basis of its 16S rRNA gene sequence, strain YIM PH21725T should belong to the genus Amycolatopsis, and was closely related to Amycolatopsis sulphurea DSM 46092T (98.57 %), Amycolatopsis jejuensis JCM13280T (97.27 %), Amycolatopsis jiangsuensis KCTC 19885T (96.88 %) and Amycolatopsis ultiminotia JCM 16989T (96.8 %).
30605074	11	59	theme	49031T=DSM	1334:1343	arg1	105902T					1345:1351	=CCTCC AA 2017044T=KCTC 49031T=DSM 105902T	1310:1351	=CCTCC AA 2017044T=KCTC 49031T=DSM 105902T	1310:1351	The type strain is YIM PH21725T (=CCTCC AA 2017044T=KCTC 49031T=DSM 105902T).
30605074	11	59	theme	49031T=DSM	1334:1343	arg1	PH21725T					1300:1307	YIM PH21725T	1296:1307	YIM PH21725T (=CCTCC AA 2017044T=KCTC 49031T=DSM 105902T)	1296:1352	The type strain is YIM PH21725T (=CCTCC AA 2017044T=KCTC 49031T=DSM 105902T).
30605074	6	60	theme	structure	625:633	arg1	phospholipids					574:586	phospholipids	574:586	phospholipids	574:586	The polar lipids included phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol mannoside, phosphatidylinositol, phospholipids and phospholipids of an unidentified structure containing glucosamine.
30605074	6	60	theme	structure	625:633	arg1	phosphatidylethanolamine					470:493	phosphatidylethanolamine	470:493	phosphatidylethanolamine	470:493	The polar lipids included phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol mannoside, phosphatidylinositol, phospholipids and phospholipids of an unidentified structure containing glucosamine.
30605074	6	60	theme	structure	625:633	arg1	phospholipids					592:604	phospholipids	592:604	phospholipids	592:604	The polar lipids included phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol mannoside, phosphatidylinositol, phospholipids and phospholipids of an unidentified structure containing glucosamine.
30605074	8	61	theme	YIM	757:759	arg1	PH21725T					761:768	strain YIM PH21725T	750:768	strain YIM PH21725T	750:768	On the basis of its 16S rRNA gene sequence, strain YIM PH21725T should belong to the genus Amycolatopsis, and was closely related to Amycolatopsis sulphurea DSM 46092T (98.57 %), Amycolatopsis jejuensis JCM13280T (97.27 %), Amycolatopsis jiangsuensis KCTC 19885T (96.88 %) and Amycolatopsis ultiminotia JCM 16989T (96.8 %).
30605074	2	62	theme	strain	127:132	arg1	PH21725T					138:145	designated strain YIM PH21725T	116:145	designated strain YIM PH21725T	116:145	A novel Gram-positive bacterium, designated strain YIM PH21725T, was isolated from a sample of rhizospheric soil of Panaxnotoginseng cultivated in Anning, Yunnan.
30605074	2	62	theme	strain	127:132	arg1	bacterium					105:113	A novel Gram-positive bacterium	83:113	A novel Gram-positive bacterium	83:113	A novel Gram-positive bacterium, designated strain YIM PH21725T, was isolated from a sample of rhizospheric soil of Panaxnotoginseng cultivated in Anning, Yunnan.
30605074	4	63	theme	fatty	333:337	arg1	acids					339:343	The main fatty acids	324:343	The main fatty acids identified	324:354	The main fatty acids identified were C17 : 0, iso-C15 : 0, iso-C16 : 0 and C16 : 0.
30605074	4	63	theme	fatty	333:337	arg1	C17 					361:364	C17 	361:364	C17 	361:364	The main fatty acids identified were C17 : 0, iso-C15 : 0, iso-C16 : 0 and C16 : 0.
30605074	11	64	theme	type	1281:1284	arg1	strain					1286:1291	The type strain	1277:1291	The type strain	1277:1291	The type strain is YIM PH21725T (=CCTCC AA 2017044T=KCTC 49031T=DSM 105902T).
30605074	11	64	theme	type	1281:1284	arg1	PH21725T					1300:1307	YIM PH21725T	1296:1307	YIM PH21725T (=CCTCC AA 2017044T=KCTC 49031T=DSM 105902T)	1296:1352	The type strain is YIM PH21725T (=CCTCC AA 2017044T=KCTC 49031T=DSM 105902T).
30605074	5	65	theme	main	412:415	arg1	MK-9					433:436	MK-9	433:436	MK-9 (H4)	433:441	The main menaquinone was MK-9 (H4).
30605074	5	65	theme	main	412:415	arg1	menaquinone					417:427	The main menaquinone	408:427	The main menaquinone	408:427	The main menaquinone was MK-9 (H4).
30605074	8	66	theme	strain	750:755	arg1	PH21725T					761:768	strain YIM PH21725T	750:768	strain YIM PH21725T	750:768	On the basis of its 16S rRNA gene sequence, strain YIM PH21725T should belong to the genus Amycolatopsis, and was closely related to Amycolatopsis sulphurea DSM 46092T (98.57 %), Amycolatopsis jejuensis JCM13280T (97.27 %), Amycolatopsis jiangsuensis KCTC 19885T (96.88 %) and Amycolatopsis ultiminotia JCM 16989T (96.8 %).
30325294	5	0	theme	rRNA	769:772	arg1	related					877:883	related	877:883	related	877:883	The result of 16S rRNA gene sequence analysis revealed the strain was a member of the genus Microbispora and was most closely related to Microbispora hainanensis 211020T (98.3 % 16S rRNA gene sequence similarity).
30325294	5	0	theme	rRNA	769:772	arg1	result					755:760	The result	751:760	The result of 16S rRNA	751:772	The result of 16S rRNA gene sequence analysis revealed the strain was a member of the genus Microbispora and was most closely related to Microbispora hainanensis 211020T (98.3 % 16S rRNA gene sequence similarity).
30325294	5	0	theme	rRNA	769:772	arg1	analysis					788:795	gene sequence analysis	774:795	gene sequence analysis	774:795	The result of 16S rRNA gene sequence analysis revealed the strain was a member of the genus Microbispora and was most closely related to Microbispora hainanensis 211020T (98.3 % 16S rRNA gene sequence similarity).
30325294	5	0	theme	rRNA	769:772	arg1	strain					810:815	the strain	806:815	the strain	806:815	The result of 16S rRNA gene sequence analysis revealed the strain was a member of the genus Microbispora and was most closely related to Microbispora hainanensis 211020T (98.3 % 16S rRNA gene sequence similarity).
30325294	5	0	theme	rRNA	769:772	arg1	member					823:828	a member	821:828	a member of the genus Microbispora	821:854	The result of 16S rRNA gene sequence analysis revealed the strain was a member of the genus Microbispora and was most closely related to Microbispora hainanensis 211020T (98.3 % 16S rRNA gene sequence similarity).
30325294	3	1	theme	chemotaxonomic	243:256	arg1	properties					272:281	chemotaxonomic and genotypic properties	243:281	chemotaxonomic and genotypic properties typical of the genus Microbispora	243:315	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30325294	5	2	theme	sequence	779:786	arg1	result					755:760	The result	751:760	The result of 16S rRNA	751:772	The result of 16S rRNA gene sequence analysis revealed the strain was a member of the genus Microbispora and was most closely related to Microbispora hainanensis 211020T (98.3 % 16S rRNA gene sequence similarity).
30325294	5	2	theme	sequence	779:786	arg1	analysis					788:795	gene sequence analysis	774:795	gene sequence analysis	774:795	The result of 16S rRNA gene sequence analysis revealed the strain was a member of the genus Microbispora and was most closely related to Microbispora hainanensis 211020T (98.3 % 16S rRNA gene sequence similarity).
30325294	5	2	theme	sequence	779:786	arg1	strain					810:815	the strain	806:815	the strain	806:815	The result of 16S rRNA gene sequence analysis revealed the strain was a member of the genus Microbispora and was most closely related to Microbispora hainanensis 211020T (98.3 % 16S rRNA gene sequence similarity).
30325294	5	2	theme	sequence	779:786	arg1	member					823:828	a member	821:828	a member of the genus Microbispora	821:854	The result of 16S rRNA gene sequence analysis revealed the strain was a member of the genus Microbispora and was most closely related to Microbispora hainanensis 211020T (98.3 % 16S rRNA gene sequence similarity).
30325294	3	3	theme	typical	283:289	arg1	properties					272:281	chemotaxonomic and genotypic properties	243:281	chemotaxonomic and genotypic properties typical of the genus Microbispora	243:315	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30325294	3	4	theme	characteristic	611:624	arg1	menaquinones					626:637	the characteristic menaquinones	607:637	the characteristic menaquinones	607:637	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30325294	8	5	theme	7648T=NBRC	1276:1285	arg1	RTBAU4-9T					1259:1267	RTBAU4-9T	1259:1267	RTBAU4-9T (=TBRC 7648T=NBRC 113147T)	1259:1294	The type strain is RTBAU4-9T (=TBRC 7648T=NBRC 113147T).
30325294	8	5	theme	7648T=NBRC	1276:1285	arg1	113147T					1287:1293	=TBRC 7648T=NBRC 113147T	1270:1293	=TBRC 7648T=NBRC 113147T	1270:1293	The type strain is RTBAU4-9T (=TBRC 7648T=NBRC 113147T).
30325294	5	6	dep	member	823:828	arg1	revealed					797:804	revealed	797:804	revealed	797:804	The result of 16S rRNA gene sequence analysis revealed the strain was a member of the genus Microbispora and was most closely related to Microbispora hainanensis 211020T (98.3 % 16S rRNA gene sequence similarity).
30325294	5	7	theme	gene	938:941	arg1	211020T					913:919	Microbispora hainanensis 211020T	888:919	Microbispora hainanensis 211020T (98.3 % 16S rRNA gene sequence similarity)	888:962	The result of 16S rRNA gene sequence analysis revealed the strain was a member of the genus Microbispora and was most closely related to Microbispora hainanensis 211020T (98.3 % 16S rRNA gene sequence similarity).
30325294	5	7	theme	gene	938:941	arg1	similarity					952:961	98.3 % 16S rRNA gene sequence similarity	922:961	98.3 % 16S rRNA gene sequence similarity	922:961	The result of 16S rRNA gene sequence analysis revealed the strain was a member of the genus Microbispora and was most closely related to Microbispora hainanensis 211020T (98.3 % 16S rRNA gene sequence similarity).
30325294	3	8	dep	exhibited	233:241	arg1	 0					673:674	 0	673:674	 0 as the main fatty acids	673:698	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30325294	3	8	dep	exhibited	233:241	arg1	anteiso-C17 					660:671	anteiso-C17 	660:671	anteiso-C17 	660:671	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30325294	3	8	dep	exhibited	233:241	arg1	 0					653:654	 0	653:654	 0	653:654	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30325294	2	9	theme	actinomycete	72:83	arg1	RTBAU4-9T					93:101	A novel actinomycete, strain RTBAU4-9T	64:101	RTBAU4-9T	93:101	A novel actinomycete, strain RTBAU4-9T, which produced longitudinally paired spores on aerial mycelia, was isolated from a soil sample of a hot spring area.
30325294	8	10	theme	=TBRC	1270:1274	arg1	RTBAU4-9T					1259:1267	RTBAU4-9T	1259:1267	RTBAU4-9T (=TBRC 7648T=NBRC 113147T)	1259:1294	The type strain is RTBAU4-9T (=TBRC 7648T=NBRC 113147T).
30325294	8	10	theme	=TBRC	1270:1274	arg1	113147T					1287:1293	=TBRC 7648T=NBRC 113147T	1270:1293	=TBRC 7648T=NBRC 113147T	1270:1293	The type strain is RTBAU4-9T (=TBRC 7648T=NBRC 113147T).
30325294	3	11	theme	phosphoglycolipids	522:539	arg1	acid					346:349	meso-diaminopimelic acid	326:349	meso-diaminopimelic acid in the cell-wall peptidoglycan	326:380	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30325294	3	11	theme	phosphoglycolipids	522:539	arg1	glucose					383:389	glucose	383:389	glucose	383:389	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30325294	3	11	theme	phosphoglycolipids	522:539	arg1	phosphatidylethanolamine					469:492	phosphatidylethanolamine	469:492	phosphatidylethanolamine	469:492	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30325294	3	11	theme	phosphoglycolipids	522:539	arg1	ribose					392:397	ribose	392:397	ribose	392:397	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30325294	3	11	theme	phosphoglycolipids	522:539	arg1	diphosphatidylglycerol					495:516	diphosphatidylglycerol	495:516	diphosphatidylglycerol	495:516	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30325294	3	11	theme	phosphoglycolipids	522:539	arg1	phosphoglycolipids					522:539	phosphoglycolipids	522:539	phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0)	522:602	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30325294	3	11	theme	phosphoglycolipids	522:539	arg1	amount					409:414	trace amount	403:414	trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0)	403:602	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30325294	3	11	theme	phosphoglycolipids	522:539	arg1	iso-C16 					644:651	iso-C16 	644:651	iso-C16 	644:651	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30325294	3	11	theme	phosphoglycolipids	522:539	arg1	madurose					419:426	madurose	419:426	madurose as the characteristic whole-cell sugars	419:466	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30325294	6	12	theme	DNA-DNA	1000:1006	arg1	relatedness					1008:1018	DNA-DNA relatedness	1000:1018	DNA-DNA relatedness (<28.1±1.2 %)	1000:1032	In addition, the low percentage of DNA-DNA relatedness (<28.1±1.2 %) and several phenotypic differences confirmed that strain RTBAU4-9T should be considered as representing a novel species of the genus Microbispora, for which the name Microbispora soli sp.
30325294	6	12	theme	DNA-DNA	1000:1006	arg1	%					1031:1031	<28.1±1.2 %	1021:1031	<28.1±1.2 %	1021:1031	In addition, the low percentage of DNA-DNA relatedness (<28.1±1.2 %) and several phenotypic differences confirmed that strain RTBAU4-9T should be considered as representing a novel species of the genus Microbispora, for which the name Microbispora soli sp.
30325294	6	13	theme	novel	1140:1144	arg1	species					1146:1152	a novel species	1138:1152	a novel species	1138:1152	In addition, the low percentage of DNA-DNA relatedness (<28.1±1.2 %) and several phenotypic differences confirmed that strain RTBAU4-9T should be considered as representing a novel species of the genus Microbispora, for which the name Microbispora soli sp.
30325294	5	14	theme	Microbispora	843:854	arg1	related					877:883	related	877:883	related	877:883	The result of 16S rRNA gene sequence analysis revealed the strain was a member of the genus Microbispora and was most closely related to Microbispora hainanensis 211020T (98.3 % 16S rRNA gene sequence similarity).
30325294	5	14	theme	Microbispora	843:854	arg1	result					755:760	The result	751:760	The result of 16S rRNA	751:772	The result of 16S rRNA gene sequence analysis revealed the strain was a member of the genus Microbispora and was most closely related to Microbispora hainanensis 211020T (98.3 % 16S rRNA gene sequence similarity).
30325294	5	14	theme	Microbispora	843:854	arg1	analysis					788:795	gene sequence analysis	774:795	gene sequence analysis	774:795	The result of 16S rRNA gene sequence analysis revealed the strain was a member of the genus Microbispora and was most closely related to Microbispora hainanensis 211020T (98.3 % 16S rRNA gene sequence similarity).
30325294	5	14	theme	Microbispora	843:854	arg1	strain					810:815	the strain	806:815	the strain	806:815	The result of 16S rRNA gene sequence analysis revealed the strain was a member of the genus Microbispora and was most closely related to Microbispora hainanensis 211020T (98.3 % 16S rRNA gene sequence similarity).
30325294	5	14	theme	Microbispora	843:854	arg1	member					823:828	a member	821:828	a member of the genus Microbispora	821:854	The result of 16S rRNA gene sequence analysis revealed the strain was a member of the genus Microbispora and was most closely related to Microbispora hainanensis 211020T (98.3 % 16S rRNA gene sequence similarity).
30325294	6	15	theme	genus	1161:1165	arg1	Microbispora					1167:1178	the genus Microbispora	1157:1178	the genus Microbispora	1157:1178	In addition, the low percentage of DNA-DNA relatedness (<28.1±1.2 %) and several phenotypic differences confirmed that strain RTBAU4-9T should be considered as representing a novel species of the genus Microbispora, for which the name Microbispora soli sp.
30325294	3	16	theme	genus	298:302	arg1	Microbispora					304:315	the genus Microbispora	294:315	the genus Microbispora	294:315	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30325294	4	17	theme	70.2 mol	740:747	arg1	%					748:748	70.2 mol%	740:748	70.2 mol%	740:748	The G+C content of the genomic DNA was 70.2 mol%.
30325294	4	17	theme	70.2 mol	740:747	arg1	content					709:715	The G+C content	701:715	The G+C content of the genomic DNA	701:734	The G+C content of the genomic DNA was 70.2 mol%.
30325294	3	18	theme	diphosphatidylglycerol	495:516	arg1	acid					346:349	meso-diaminopimelic acid	326:349	meso-diaminopimelic acid in the cell-wall peptidoglycan	326:380	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30325294	3	18	theme	diphosphatidylglycerol	495:516	arg1	glucose					383:389	glucose	383:389	glucose	383:389	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30325294	3	18	theme	diphosphatidylglycerol	495:516	arg1	phosphatidylethanolamine					469:492	phosphatidylethanolamine	469:492	phosphatidylethanolamine	469:492	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30325294	3	18	theme	diphosphatidylglycerol	495:516	arg1	ribose					392:397	ribose	392:397	ribose	392:397	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30325294	3	18	theme	diphosphatidylglycerol	495:516	arg1	diphosphatidylglycerol					495:516	diphosphatidylglycerol	495:516	diphosphatidylglycerol	495:516	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30325294	3	18	theme	diphosphatidylglycerol	495:516	arg1	phosphoglycolipids					522:539	phosphoglycolipids	522:539	phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0)	522:602	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30325294	3	18	theme	diphosphatidylglycerol	495:516	arg1	amount					409:414	trace amount	403:414	trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0)	403:602	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30325294	3	18	theme	diphosphatidylglycerol	495:516	arg1	iso-C16 					644:651	iso-C16 	644:651	iso-C16 	644:651	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30325294	3	18	theme	diphosphatidylglycerol	495:516	arg1	madurose					419:426	madurose	419:426	madurose as the characteristic whole-cell sugars	419:466	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30325294	3	19	theme	Microbispora	304:315	arg1	typical					283:289	typical	283:289	typical	283:289	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30325294	3	20	from	acid	346:349	arg1	peptidoglycan					368:380	the cell-wall peptidoglycan	354:380	the cell-wall peptidoglycan	354:380	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30325294	1	21	theme	hot	52:54	arg1	spring					56:61	a hot spring	50:61	a hot spring	50:61	nov., isolated from soil of a hot spring.
30325294	2	22	attach	isolated	171:178	arg1	sample					192:197	a soil sample	185:197	a soil sample of a hot spring area	185:218	A novel actinomycete, strain RTBAU4-9T, which produced longitudinally paired spores on aerial mycelia, was isolated from a soil sample of a hot spring area.
30325294	2	22	attach	isolated	171:178	arg2	RTBAU4-9T					93:101	A novel actinomycete, strain RTBAU4-9T	64:101	RTBAU4-9T	93:101	A novel actinomycete, strain RTBAU4-9T, which produced longitudinally paired spores on aerial mycelia, was isolated from a soil sample of a hot spring area.
30325294	0	23	theme	soli	13:16	arg1	sp					18:19	Microbispora soli sp	0:19	Microbispora soli sp.	0:20	Microbispora soli sp.
30325294	8	24	theme	type	1244:1247	arg1	RTBAU4-9T					1259:1267	RTBAU4-9T	1259:1267	RTBAU4-9T (=TBRC 7648T=NBRC 113147T)	1259:1294	The type strain is RTBAU4-9T (=TBRC 7648T=NBRC 113147T).
30325294	8	24	theme	type	1244:1247	arg1	strain					1249:1254	The type strain	1240:1254	The type strain	1240:1254	The type strain is RTBAU4-9T (=TBRC 7648T=NBRC 113147T).
30325294	1	25	theme	spring	56:61	arg1	soil					42:45	soil	42:45	soil of a hot spring	42:61	nov., isolated from soil of a hot spring.
30325294	0	26	theme	Microbispora	0:11	arg1	sp					18:19	Microbispora soli sp	0:19	Microbispora soli sp.	0:20	Microbispora soli sp.
30325294	5	27	theme	16S	765:767	arg1	rRNA					769:772	16S rRNA	765:772	16S rRNA	765:772	The result of 16S rRNA gene sequence analysis revealed the strain was a member of the genus Microbispora and was most closely related to Microbispora hainanensis 211020T (98.3 % 16S rRNA gene sequence similarity).
30325294	6	28	theme	Microbispora	1200:1211	arg1	sp					1218:1219	the name Microbispora soli sp	1191:1219	the name Microbispora soli sp	1191:1219	In addition, the low percentage of DNA-DNA relatedness (<28.1±1.2 %) and several phenotypic differences confirmed that strain RTBAU4-9T should be considered as representing a novel species of the genus Microbispora, for which the name Microbispora soli sp.
30325294	6	29	theme	strain	1084:1089	arg1	RTBAU4-9T					1091:1099	strain RTBAU4-9T	1084:1099	strain RTBAU4-9T	1084:1099	In addition, the low percentage of DNA-DNA relatedness (<28.1±1.2 %) and several phenotypic differences confirmed that strain RTBAU4-9T should be considered as representing a novel species of the genus Microbispora, for which the name Microbispora soli sp.
30325294	6	30	theme	relatedness	1008:1018	arg1	percentage					986:995	the low percentage	978:995	the low percentage of DNA-DNA relatedness (<28.1±1.2 %) and several phenotypic differences	978:1067	In addition, the low percentage of DNA-DNA relatedness (<28.1±1.2 %) and several phenotypic differences confirmed that strain RTBAU4-9T should be considered as representing a novel species of the genus Microbispora, for which the name Microbispora soli sp.
30325294	5	31	theme	genus	837:841	arg1	Microbispora					843:854	the genus Microbispora	833:854	the genus Microbispora	833:854	The result of 16S rRNA gene sequence analysis revealed the strain was a member of the genus Microbispora and was most closely related to Microbispora hainanensis 211020T (98.3 % 16S rRNA gene sequence similarity).
30325294	3	32	theme	meso-diaminopimelic	326:344	arg1	acid					346:349	meso-diaminopimelic acid	326:349	meso-diaminopimelic acid in the cell-wall peptidoglycan	326:380	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30325294	6	33	theme	several	1038:1044	arg1	differences					1057:1067	several phenotypic differences	1038:1067	several phenotypic differences	1038:1067	In addition, the low percentage of DNA-DNA relatedness (<28.1±1.2 %) and several phenotypic differences confirmed that strain RTBAU4-9T should be considered as representing a novel species of the genus Microbispora, for which the name Microbispora soli sp.
30325294	6	34	theme	name	1195:1198	arg1	sp					1218:1219	the name Microbispora soli sp	1191:1219	the name Microbispora soli sp	1191:1219	In addition, the low percentage of DNA-DNA relatedness (<28.1±1.2 %) and several phenotypic differences confirmed that strain RTBAU4-9T should be considered as representing a novel species of the genus Microbispora, for which the name Microbispora soli sp.
30325294	2	35	theme	aerial	151:156	arg1	mycelia					158:164	aerial mycelia	151:164	aerial mycelia	151:164	A novel actinomycete, strain RTBAU4-9T, which produced longitudinally paired spores on aerial mycelia, was isolated from a soil sample of a hot spring area.
30325294	3	36	theme	membrane	548:555	arg1	MK-9					595:598	MK-9	595:598	MK-9	595:598	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30325294	3	36	theme	membrane	548:555	arg1	MK-9					582:585	MK-9	582:585	MK-9	582:585	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30325294	3	36	theme	membrane	548:555	arg1	MK-9					572:575	MK-9	572:575	MK-9	572:575	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30325294	3	36	theme	membrane	548:555	arg1	phospholipids					557:569	the membrane phospholipids	544:569	the membrane phospholipids	544:569	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30325294	3	37	theme	madurose	419:426	arg1	acid					346:349	meso-diaminopimelic acid	326:349	meso-diaminopimelic acid in the cell-wall peptidoglycan	326:380	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30325294	3	37	theme	madurose	419:426	arg1	glucose					383:389	glucose	383:389	glucose	383:389	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30325294	3	37	theme	madurose	419:426	arg1	phosphatidylethanolamine					469:492	phosphatidylethanolamine	469:492	phosphatidylethanolamine	469:492	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30325294	3	37	theme	madurose	419:426	arg1	ribose					392:397	ribose	392:397	ribose	392:397	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30325294	3	37	theme	madurose	419:426	arg1	diphosphatidylglycerol					495:516	diphosphatidylglycerol	495:516	diphosphatidylglycerol	495:516	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30325294	3	37	theme	madurose	419:426	arg1	phosphoglycolipids					522:539	phosphoglycolipids	522:539	phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0)	522:602	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30325294	3	37	theme	madurose	419:426	arg1	amount					409:414	trace amount	403:414	trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0)	403:602	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30325294	3	37	theme	madurose	419:426	arg1	iso-C16 					644:651	iso-C16 	644:651	iso-C16 	644:651	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30325294	3	37	theme	madurose	419:426	arg1	madurose					419:426	madurose	419:426	madurose as the characteristic whole-cell sugars	419:466	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30325294	5	38	theme	sequence	943:950	arg1	211020T					913:919	Microbispora hainanensis 211020T	888:919	Microbispora hainanensis 211020T (98.3 % 16S rRNA gene sequence similarity)	888:962	The result of 16S rRNA gene sequence analysis revealed the strain was a member of the genus Microbispora and was most closely related to Microbispora hainanensis 211020T (98.3 % 16S rRNA gene sequence similarity).
30325294	5	38	theme	sequence	943:950	arg1	similarity					952:961	98.3 % 16S rRNA gene sequence similarity	922:961	98.3 % 16S rRNA gene sequence similarity	922:961	The result of 16S rRNA gene sequence analysis revealed the strain was a member of the genus Microbispora and was most closely related to Microbispora hainanensis 211020T (98.3 % 16S rRNA gene sequence similarity).
30325294	5	39	theme	Microbispora	888:899	arg1	similarity					952:961	98.3 % 16S rRNA gene sequence similarity	922:961	98.3 % 16S rRNA gene sequence similarity	922:961	The result of 16S rRNA gene sequence analysis revealed the strain was a member of the genus Microbispora and was most closely related to Microbispora hainanensis 211020T (98.3 % 16S rRNA gene sequence similarity).
30325294	5	39	theme	Microbispora	888:899	arg1	211020T					913:919	Microbispora hainanensis 211020T	888:919	Microbispora hainanensis 211020T (98.3 % 16S rRNA gene sequence similarity)	888:962	The result of 16S rRNA gene sequence analysis revealed the strain was a member of the genus Microbispora and was most closely related to Microbispora hainanensis 211020T (98.3 % 16S rRNA gene sequence similarity).
30325294	6	40	theme	phenotypic	1046:1055	arg1	differences					1057:1067	several phenotypic differences	1038:1067	several phenotypic differences	1038:1067	In addition, the low percentage of DNA-DNA relatedness (<28.1±1.2 %) and several phenotypic differences confirmed that strain RTBAU4-9T should be considered as representing a novel species of the genus Microbispora, for which the name Microbispora soli sp.
30325294	3	41	theme	trace	403:407	arg1	phosphoglycolipids					522:539	phosphoglycolipids	522:539	phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0)	522:602	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30325294	3	41	theme	trace	403:407	arg1	amount					409:414	trace amount	403:414	trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0)	403:602	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30325294	3	41	theme	trace	403:407	arg1	diphosphatidylglycerol					495:516	diphosphatidylglycerol	495:516	diphosphatidylglycerol	495:516	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30325294	3	41	theme	trace	403:407	arg1	phosphatidylethanolamine					469:492	phosphatidylethanolamine	469:492	phosphatidylethanolamine	469:492	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30325294	3	41	theme	trace	403:407	arg1	madurose					419:426	madurose	419:426	madurose as the characteristic whole-cell sugars	419:466	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30325294	3	42	theme	fatty	688:692	arg1	acids					694:698	the main fatty acids	679:698	the main fatty acids	679:698	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30325294	2	43	theme	soil	187:190	arg1	sample					192:197	a soil sample	185:197	a soil sample of a hot spring area	185:218	A novel actinomycete, strain RTBAU4-9T, which produced longitudinally paired spores on aerial mycelia, was isolated from a soil sample of a hot spring area.
30325294	6	44	theme	soli	1213:1216	arg1	sp					1218:1219	the name Microbispora soli sp	1191:1219	the name Microbispora soli sp	1191:1219	In addition, the low percentage of DNA-DNA relatedness (<28.1±1.2 %) and several phenotypic differences confirmed that strain RTBAU4-9T should be considered as representing a novel species of the genus Microbispora, for which the name Microbispora soli sp.
30325294	2	45	theme	novel	66:70	arg1	RTBAU4-9T					93:101	A novel actinomycete, strain RTBAU4-9T	64:101	RTBAU4-9T	93:101	A novel actinomycete, strain RTBAU4-9T, which produced longitudinally paired spores on aerial mycelia, was isolated from a soil sample of a hot spring area.
30325294	2	46	theme	paired	134:139	arg1	spores					141:146	longitudinally paired spores	119:146	longitudinally paired spores	119:146	A novel actinomycete, strain RTBAU4-9T, which produced longitudinally paired spores on aerial mycelia, was isolated from a soil sample of a hot spring area.
30325294	4	47	theme	DNA	732:734	arg1	%					748:748	70.2 mol%	740:748	70.2 mol%	740:748	The G+C content of the genomic DNA was 70.2 mol%.
30325294	4	47	theme	DNA	732:734	arg1	content					709:715	The G+C content	701:715	The G+C content of the genomic DNA	701:734	The G+C content of the genomic DNA was 70.2 mol%.
30325294	3	48	from	amount	409:414	arg1	peptidoglycan					368:380	the cell-wall peptidoglycan	354:380	the cell-wall peptidoglycan	354:380	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30325294	3	49	theme	characteristic	435:448	arg1	sugars					461:466	the characteristic whole-cell sugars	431:466	the characteristic whole-cell sugars	431:466	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30325294	3	50	theme	cell-wall	358:366	arg1	peptidoglycan					368:380	the cell-wall peptidoglycan	354:380	the cell-wall peptidoglycan	354:380	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30325294	2	51	dep	RTBAU4-9T	93:101	arg1	strain					86:91	A novel actinomycete, strain RTBAU4-9T	64:101	strain	86:91	A novel actinomycete, strain RTBAU4-9T, which produced longitudinally paired spores on aerial mycelia, was isolated from a soil sample of a hot spring area.
30325294	3	52	theme	genotypic	262:270	arg1	properties					272:281	chemotaxonomic and genotypic properties	243:281	chemotaxonomic and genotypic properties typical of the genus Microbispora	243:315	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30325294	5	53	theme	98.3 	922:926	arg1	%					927:927	%	927:927	%	927:927	The result of 16S rRNA gene sequence analysis revealed the strain was a member of the genus Microbispora and was most closely related to Microbispora hainanensis 211020T (98.3 % 16S rRNA gene sequence similarity).
30325294	4	54	theme	genomic	724:730	arg1	DNA					732:734	the genomic DNA	720:734	the genomic DNA	720:734	The G+C content of the genomic DNA was 70.2 mol%.
30325294	3	55	theme	whole-cell	450:459	arg1	sugars					461:466	the characteristic whole-cell sugars	431:466	the characteristic whole-cell sugars	431:466	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30325294	3	56	theme	main	683:686	arg1	acids					694:698	the main fatty acids	679:698	the main fatty acids	679:698	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30325294	6	57	theme	Microbispora	1167:1178	arg1	species					1146:1152	a novel species	1138:1152	a novel species	1138:1152	In addition, the low percentage of DNA-DNA relatedness (<28.1±1.2 %) and several phenotypic differences confirmed that strain RTBAU4-9T should be considered as representing a novel species of the genus Microbispora, for which the name Microbispora soli sp.
30325294	5	58	theme	%	927:927	arg1	211020T					913:919	Microbispora hainanensis 211020T	888:919	Microbispora hainanensis 211020T (98.3 % 16S rRNA gene sequence similarity)	888:962	The result of 16S rRNA gene sequence analysis revealed the strain was a member of the genus Microbispora and was most closely related to Microbispora hainanensis 211020T (98.3 % 16S rRNA gene sequence similarity).
30325294	5	58	theme	%	927:927	arg1	similarity					952:961	98.3 % 16S rRNA gene sequence similarity	922:961	98.3 % 16S rRNA gene sequence similarity	922:961	The result of 16S rRNA gene sequence analysis revealed the strain was a member of the genus Microbispora and was most closely related to Microbispora hainanensis 211020T (98.3 % 16S rRNA gene sequence similarity).
30325294	2	59	theme	spring	208:213	arg1	area					215:218	a hot spring area	202:218	a hot spring area	202:218	A novel actinomycete, strain RTBAU4-9T, which produced longitudinally paired spores on aerial mycelia, was isolated from a soil sample of a hot spring area.
30325294	5	60	theme	hainanensis	901:911	arg1	similarity					952:961	98.3 % 16S rRNA gene sequence similarity	922:961	98.3 % 16S rRNA gene sequence similarity	922:961	The result of 16S rRNA gene sequence analysis revealed the strain was a member of the genus Microbispora and was most closely related to Microbispora hainanensis 211020T (98.3 % 16S rRNA gene sequence similarity).
30325294	5	60	theme	hainanensis	901:911	arg1	211020T					913:919	Microbispora hainanensis 211020T	888:919	Microbispora hainanensis 211020T (98.3 % 16S rRNA gene sequence similarity)	888:962	The result of 16S rRNA gene sequence analysis revealed the strain was a member of the genus Microbispora and was most closely related to Microbispora hainanensis 211020T (98.3 % 16S rRNA gene sequence similarity).
30325294	6	61	theme	low	982:984	arg1	percentage					986:995	the low percentage	978:995	the low percentage of DNA-DNA relatedness (<28.1±1.2 %) and several phenotypic differences	978:1067	In addition, the low percentage of DNA-DNA relatedness (<28.1±1.2 %) and several phenotypic differences confirmed that strain RTBAU4-9T should be considered as representing a novel species of the genus Microbispora, for which the name Microbispora soli sp.
30325294	2	62	theme	area	215:218	arg1	sample					192:197	a soil sample	185:197	a soil sample of a hot spring area	185:218	A novel actinomycete, strain RTBAU4-9T, which produced longitudinally paired spores on aerial mycelia, was isolated from a soil sample of a hot spring area.
30325294	5	63	theme	16S	929:931	arg1	211020T					913:919	Microbispora hainanensis 211020T	888:919	Microbispora hainanensis 211020T (98.3 % 16S rRNA gene sequence similarity)	888:962	The result of 16S rRNA gene sequence analysis revealed the strain was a member of the genus Microbispora and was most closely related to Microbispora hainanensis 211020T (98.3 % 16S rRNA gene sequence similarity).
30325294	5	63	theme	16S	929:931	arg1	similarity					952:961	98.3 % 16S rRNA gene sequence similarity	922:961	98.3 % 16S rRNA gene sequence similarity	922:961	The result of 16S rRNA gene sequence analysis revealed the strain was a member of the genus Microbispora and was most closely related to Microbispora hainanensis 211020T (98.3 % 16S rRNA gene sequence similarity).
30325294	2	64	theme	hot	204:206	arg1	area					215:218	a hot spring area	202:218	a hot spring area	202:218	A novel actinomycete, strain RTBAU4-9T, which produced longitudinally paired spores on aerial mycelia, was isolated from a soil sample of a hot spring area.
30325294	3	65	from	glucose	383:389	arg1	peptidoglycan					368:380	the cell-wall peptidoglycan	354:380	the cell-wall peptidoglycan	354:380	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30325294	5	66	theme	gene	774:777	arg1	result					755:760	The result	751:760	The result of 16S rRNA	751:772	The result of 16S rRNA gene sequence analysis revealed the strain was a member of the genus Microbispora and was most closely related to Microbispora hainanensis 211020T (98.3 % 16S rRNA gene sequence similarity).
30325294	5	66	theme	gene	774:777	arg1	analysis					788:795	gene sequence analysis	774:795	gene sequence analysis	774:795	The result of 16S rRNA gene sequence analysis revealed the strain was a member of the genus Microbispora and was most closely related to Microbispora hainanensis 211020T (98.3 % 16S rRNA gene sequence similarity).
30325294	5	66	theme	gene	774:777	arg1	strain					810:815	the strain	806:815	the strain	806:815	The result of 16S rRNA gene sequence analysis revealed the strain was a member of the genus Microbispora and was most closely related to Microbispora hainanensis 211020T (98.3 % 16S rRNA gene sequence similarity).
30325294	5	66	theme	gene	774:777	arg1	member					823:828	a member	821:828	a member of the genus Microbispora	821:854	The result of 16S rRNA gene sequence analysis revealed the strain was a member of the genus Microbispora and was most closely related to Microbispora hainanensis 211020T (98.3 % 16S rRNA gene sequence similarity).
30325294	5	67	theme	rRNA	933:936	arg1	211020T					913:919	Microbispora hainanensis 211020T	888:919	Microbispora hainanensis 211020T (98.3 % 16S rRNA gene sequence similarity)	888:962	The result of 16S rRNA gene sequence analysis revealed the strain was a member of the genus Microbispora and was most closely related to Microbispora hainanensis 211020T (98.3 % 16S rRNA gene sequence similarity).
30325294	5	67	theme	rRNA	933:936	arg1	similarity					952:961	98.3 % 16S rRNA gene sequence similarity	922:961	98.3 % 16S rRNA gene sequence similarity	922:961	The result of 16S rRNA gene sequence analysis revealed the strain was a member of the genus Microbispora and was most closely related to Microbispora hainanensis 211020T (98.3 % 16S rRNA gene sequence similarity).
30325294	6	68	theme	differences	1057:1067	arg1	percentage					986:995	the low percentage	978:995	the low percentage of DNA-DNA relatedness (<28.1±1.2 %) and several phenotypic differences	978:1067	In addition, the low percentage of DNA-DNA relatedness (<28.1±1.2 %) and several phenotypic differences confirmed that strain RTBAU4-9T should be considered as representing a novel species of the genus Microbispora, for which the name Microbispora soli sp.
30325294	3	69	theme	phosphatidylethanolamine	469:492	arg1	acid					346:349	meso-diaminopimelic acid	326:349	meso-diaminopimelic acid in the cell-wall peptidoglycan	326:380	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30325294	3	69	theme	phosphatidylethanolamine	469:492	arg1	glucose					383:389	glucose	383:389	glucose	383:389	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30325294	3	69	theme	phosphatidylethanolamine	469:492	arg1	phosphatidylethanolamine					469:492	phosphatidylethanolamine	469:492	phosphatidylethanolamine	469:492	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30325294	3	69	theme	phosphatidylethanolamine	469:492	arg1	ribose					392:397	ribose	392:397	ribose	392:397	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30325294	3	69	theme	phosphatidylethanolamine	469:492	arg1	diphosphatidylglycerol					495:516	diphosphatidylglycerol	495:516	diphosphatidylglycerol	495:516	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30325294	3	69	theme	phosphatidylethanolamine	469:492	arg1	phosphoglycolipids					522:539	phosphoglycolipids	522:539	phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0)	522:602	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30325294	3	69	theme	phosphatidylethanolamine	469:492	arg1	amount					409:414	trace amount	403:414	trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0)	403:602	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30325294	3	69	theme	phosphatidylethanolamine	469:492	arg1	iso-C16 					644:651	iso-C16 	644:651	iso-C16 	644:651	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30325294	3	69	theme	phosphatidylethanolamine	469:492	arg1	madurose					419:426	madurose	419:426	madurose as the characteristic whole-cell sugars	419:466	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30325294	4	70	theme	G+C	705:707	arg1	%					748:748	70.2 mol%	740:748	70.2 mol%	740:748	The G+C content of the genomic DNA was 70.2 mol%.
30325294	4	70	theme	G+C	705:707	arg1	content					709:715	The G+C content	701:715	The G+C content of the genomic DNA	701:734	The G+C content of the genomic DNA was 70.2 mol%.
30325294	3	71	from	ribose	392:397	arg1	peptidoglycan					368:380	the cell-wall peptidoglycan	354:380	the cell-wall peptidoglycan	354:380	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30325294	3	72	from	iso-C16 	644:651	arg1	peptidoglycan					368:380	the cell-wall peptidoglycan	354:380	the cell-wall peptidoglycan	354:380	This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H4), MK-9(H2) and MK-9(H0) as the characteristic menaquinones, and iso-C16 : 0 and anteiso-C17 : 0 as the main fatty acids.
30189316	1	0	theme	apoptosis	139:147	arg1	improvement					153:163	improvement	153:163	improvement of mitochondrial functionality	153:194	Part 1: Enhancement of cellular viability, regulation of cellular apoptosis and improvement of mitochondrial functionality.
30189316	1	0	theme	apoptosis	139:147	arg1	regulation					116:125	regulation	116:125	regulation of cellular apoptosis	116:147	Part 1: Enhancement of cellular viability, regulation of cellular apoptosis and improvement of mitochondrial functionality.
30189316	1	0	theme	apoptosis	139:147	arg1	Enhancement					81:91	Enhancement	81:91	Enhancement of cellular viability	81:113	Part 1: Enhancement of cellular viability, regulation of cellular apoptosis and improvement of mitochondrial functionality.
30189316	7	1	theme	energetic	1433:1441	arg1	metabolism					1443:1452	energetic metabolism	1433:1452	energetic metabolism	1433:1452	In conclusion, one of the possible mechanisms by which MH exerts its beneficial effects could be to its capacity to improve cellular viability, promote proliferation and enhance energetic metabolism, by modulating the expression of several proteins involved in apoptosis, inflammation, metabolism and mitochondrial biogenesis.
30189316	0	2	from	effects	11:17	arg1	macrophages					60:70	LPS-treated RAW 264.7 macrophages	38:70	LPS-treated RAW 264.7 macrophages	38:70	Protective effects of Manuka honey on LPS-treated RAW 264.7 macrophages.
30189316	3	3	from	apoptosis	542:550	arg1	macrophages					613:623	lipopolysaccharide (LPS)-treated RAW 264.7 macrophages	570:623	lipopolysaccharide (LPS)-treated RAW 264.7 macrophages	570:623	The aim of this work was to assess the phenolic composition and the total antioxidant capacity (TAC) of MH, as well as its effects on cellular viability, proliferation, apoptosis and metabolism in lipopolysaccharide (LPS)-treated RAW 264.7 macrophages, highlighting the molecular mechanisms involved.
30189316	5	4	from	addition	800:807	arg1	able					817:820	able	817:820	able	817:820	In addition, MH was able to enhance cellular viability, decrease apoptosis, promote wound healing and attenuate inflammation in a dose-dependent manner, by reducing the expression of caspase 3, p-p38 and p-Erk1/2 proteins, in macrophages stressed with LPS.
30189316	5	5	theme	caspase	980:986	arg1	expression					966:975	the expression	962:975	the expression of caspase 3	962:988	In addition, MH was able to enhance cellular viability, decrease apoptosis, promote wound healing and attenuate inflammation in a dose-dependent manner, by reducing the expression of caspase 3, p-p38 and p-Erk1/2 proteins, in macrophages stressed with LPS.
30189316	6	6	theme	treatment	1244:1252	arg1	effects					1229:1235	the deleterious effects	1213:1235	the deleterious effects of LPS treatment	1213:1252	In addition, it improved mitochondrial respiration and glycolytic activities, stimulating the expression of p-AMPK, SIRT1 and PGC1α, counteracting in this way the deleterious effects of LPS treatment.
30189316	2	7	dep	well-known	269:278	arg1	honey					231:235	a monofloral honey	218:235	a monofloral honey from Australia and New Zealand	218:266	Manuka honey (MH) is a monofloral honey from Australia and New Zealand, well-known for its healthy properties, such as antioxidant, antimicrobial and wound healing capacities.
30189316	2	7	dep	well-known	269:278	arg1	honey					204:208	Manuka honey	197:208	Manuka honey (MH)	197:213	Manuka honey (MH) is a monofloral honey from Australia and New Zealand, well-known for its healthy properties, such as antioxidant, antimicrobial and wound healing capacities.
30189316	5	8	from	macrophages	1023:1033	arg1	able					817:820	able	817:820	able	817:820	In addition, MH was able to enhance cellular viability, decrease apoptosis, promote wound healing and attenuate inflammation in a dose-dependent manner, by reducing the expression of caspase 3, p-p38 and p-Erk1/2 proteins, in macrophages stressed with LPS.
30189316	6	9	theme	glycolytic	1109:1118	arg1	activities					1120:1129	glycolytic activities	1109:1129	glycolytic activities	1109:1129	In addition, it improved mitochondrial respiration and glycolytic activities, stimulating the expression of p-AMPK, SIRT1 and PGC1α, counteracting in this way the deleterious effects of LPS treatment.
30189316	6	10	theme	LPS	1240:1242	arg1	treatment					1244:1252	LPS treatment	1240:1252	LPS treatment	1240:1252	In addition, it improved mitochondrial respiration and glycolytic activities, stimulating the expression of p-AMPK, SIRT1 and PGC1α, counteracting in this way the deleterious effects of LPS treatment.
30189316	7	11	theme	beneficial	1324:1333	arg1	effects					1335:1341	its beneficial effects	1320:1341	its beneficial effects	1320:1341	In conclusion, one of the possible mechanisms by which MH exerts its beneficial effects could be to its capacity to improve cellular viability, promote proliferation and enhance energetic metabolism, by modulating the expression of several proteins involved in apoptosis, inflammation, metabolism and mitochondrial biogenesis.
30189316	4	12	theme	remarkable	781:790	arg1	TAC					792:794	remarkable TAC	781:794	remarkable TAC	781:794	Up to 18 compounds were identified in MH, with gallic acid and quercetin as the major ones; MH showed also remarkable TAC.
30189316	6	13	theme	p-AMPK	1162:1167	arg1	expression					1148:1157	the expression	1144:1157	the expression of p-AMPK, SIRT1 and PGC1α	1144:1184	In addition, it improved mitochondrial respiration and glycolytic activities, stimulating the expression of p-AMPK, SIRT1 and PGC1α, counteracting in this way the deleterious effects of LPS treatment.
30189316	2	14	dep	antioxidant	316:326	arg1	capacities					361:370	capacities	361:370	capacities	361:370	Manuka honey (MH) is a monofloral honey from Australia and New Zealand, well-known for its healthy properties, such as antioxidant, antimicrobial and wound healing capacities.
30189316	3	15	theme	antioxidant	447:457	arg1	TAC					469:471	TAC	469:471	TAC	469:471	The aim of this work was to assess the phenolic composition and the total antioxidant capacity (TAC) of MH, as well as its effects on cellular viability, proliferation, apoptosis and metabolism in lipopolysaccharide (LPS)-treated RAW 264.7 macrophages, highlighting the molecular mechanisms involved.
30189316	3	15	theme	antioxidant	447:457	arg1	capacity					459:466	the total antioxidant capacity	437:466	the total antioxidant capacity (TAC) of MH	437:478	The aim of this work was to assess the phenolic composition and the total antioxidant capacity (TAC) of MH, as well as its effects on cellular viability, proliferation, apoptosis and metabolism in lipopolysaccharide (LPS)-treated RAW 264.7 macrophages, highlighting the molecular mechanisms involved.
30189316	2	16	theme	New	256:258	arg1	Zealand					260:266	New Zealand	256:266	New Zealand	256:266	Manuka honey (MH) is a monofloral honey from Australia and New Zealand, well-known for its healthy properties, such as antioxidant, antimicrobial and wound healing capacities.
30189316	3	17	theme	work	389:392	arg1	aim					377:379	The aim	373:379	The aim of this work	373:392	The aim of this work was to assess the phenolic composition and the total antioxidant capacity (TAC) of MH, as well as its effects on cellular viability, proliferation, apoptosis and metabolism in lipopolysaccharide (LPS)-treated RAW 264.7 macrophages, highlighting the molecular mechanisms involved.
30189316	4	18	dep	18	680:681	arg1	to					677:678	to	677:678	to	677:678	Up to 18 compounds were identified in MH, with gallic acid and quercetin as the major ones; MH showed also remarkable TAC.
30189316	3	19	theme	molecular	643:651	arg1	mechanisms					653:662	the molecular mechanisms	639:662	the molecular mechanisms involved	639:671	The aim of this work was to assess the phenolic composition and the total antioxidant capacity (TAC) of MH, as well as its effects on cellular viability, proliferation, apoptosis and metabolism in lipopolysaccharide (LPS)-treated RAW 264.7 macrophages, highlighting the molecular mechanisms involved.
30189316	4	20	theme	major	754:758	arg1	compounds					683:691	Up to 18 compounds	674:691	Up to 18 compounds	674:691	Up to 18 compounds were identified in MH, with gallic acid and quercetin as the major ones; MH showed also remarkable TAC.
30189316	4	20	theme	major	754:758	arg1	ones					760:763	the major ones	750:763	the major ones	750:763	Up to 18 compounds were identified in MH, with gallic acid and quercetin as the major ones; MH showed also remarkable TAC.
30189316	5	21	from	able	817:820	arg1	addition					800:807	addition	800:807	addition	800:807	In addition, MH was able to enhance cellular viability, decrease apoptosis, promote wound healing and attenuate inflammation in a dose-dependent manner, by reducing the expression of caspase 3, p-p38 and p-Erk1/2 proteins, in macrophages stressed with LPS.
30189316	5	21	from	able	817:820	arg1	macrophages					1023:1033	macrophages	1023:1033	macrophages stressed with LPS	1023:1051	In addition, MH was able to enhance cellular viability, decrease apoptosis, promote wound healing and attenuate inflammation in a dose-dependent manner, by reducing the expression of caspase 3, p-p38 and p-Erk1/2 proteins, in macrophages stressed with LPS.
30189316	7	22	theme	mitochondrial	1556:1568	arg1	biogenesis					1570:1579	mitochondrial biogenesis	1556:1579	mitochondrial biogenesis	1556:1579	In conclusion, one of the possible mechanisms by which MH exerts its beneficial effects could be to its capacity to improve cellular viability, promote proliferation and enhance energetic metabolism, by modulating the expression of several proteins involved in apoptosis, inflammation, metabolism and mitochondrial biogenesis.
30189316	2	23	theme	healthy	288:294	arg1	properties					296:305	its healthy properties	284:305	its healthy properties	284:305	Manuka honey (MH) is a monofloral honey from Australia and New Zealand, well-known for its healthy properties, such as antioxidant, antimicrobial and wound healing capacities.
30189316	2	23	theme	healthy	288:294	arg1	antimicrobial					329:341	antimicrobial	329:341	antimicrobial	329:341	Manuka honey (MH) is a monofloral honey from Australia and New Zealand, well-known for its healthy properties, such as antioxidant, antimicrobial and wound healing capacities.
30189316	2	23	theme	healthy	288:294	arg1	healing					353:359	wound healing	347:359	wound healing	347:359	Manuka honey (MH) is a monofloral honey from Australia and New Zealand, well-known for its healthy properties, such as antioxidant, antimicrobial and wound healing capacities.
30189316	2	23	theme	healthy	288:294	arg1	antioxidant					316:326	antioxidant	316:326	antioxidant	316:326	Manuka honey (MH) is a monofloral honey from Australia and New Zealand, well-known for its healthy properties, such as antioxidant, antimicrobial and wound healing capacities.
30189316	3	24	from	metabolism	556:565	arg1	macrophages					613:623	lipopolysaccharide (LPS)-treated RAW 264.7 macrophages	570:623	lipopolysaccharide (LPS)-treated RAW 264.7 macrophages	570:623	The aim of this work was to assess the phenolic composition and the total antioxidant capacity (TAC) of MH, as well as its effects on cellular viability, proliferation, apoptosis and metabolism in lipopolysaccharide (LPS)-treated RAW 264.7 macrophages, highlighting the molecular mechanisms involved.
30189316	1	25	theme	mitochondrial	168:180	arg1	functionality					182:194	mitochondrial functionality	168:194	mitochondrial functionality	168:194	Part 1: Enhancement of cellular viability, regulation of cellular apoptosis and improvement of mitochondrial functionality.
30189316	0	26	theme	Protective	0:9	arg1	effects					11:17	Protective effects	0:17	Protective effects of Manuka honey on LPS-treated RAW 264.7 macrophages	0:70	Protective effects of Manuka honey on LPS-treated RAW 264.7 macrophages.
30189316	5	27	theme	cellular	833:840	arg1	viability					842:850	cellular viability	833:850	cellular viability	833:850	In addition, MH was able to enhance cellular viability, decrease apoptosis, promote wound healing and attenuate inflammation in a dose-dependent manner, by reducing the expression of caspase 3, p-p38 and p-Erk1/2 proteins, in macrophages stressed with LPS.
30189316	2	28	theme	wound	347:351	arg1	healing					353:359	wound healing	347:359	wound healing	347:359	Manuka honey (MH) is a monofloral honey from Australia and New Zealand, well-known for its healthy properties, such as antioxidant, antimicrobial and wound healing capacities.
30189316	0	29	theme	Manuka	22:27	arg1	honey					29:33	Manuka honey	22:33	Manuka honey	22:33	Protective effects of Manuka honey on LPS-treated RAW 264.7 macrophages.
30189316	6	30	theme	deleterious	1217:1227	arg1	effects					1229:1235	the deleterious effects	1213:1235	the deleterious effects of LPS treatment	1213:1252	In addition, it improved mitochondrial respiration and glycolytic activities, stimulating the expression of p-AMPK, SIRT1 and PGC1α, counteracting in this way the deleterious effects of LPS treatment.
30189316	3	31	from	capacity	459:466	arg1	apoptosis					542:550	apoptosis	542:550	apoptosis	542:550	The aim of this work was to assess the phenolic composition and the total antioxidant capacity (TAC) of MH, as well as its effects on cellular viability, proliferation, apoptosis and metabolism in lipopolysaccharide (LPS)-treated RAW 264.7 macrophages, highlighting the molecular mechanisms involved.
30189316	3	31	from	capacity	459:466	arg1	proliferation					527:539	proliferation	527:539	proliferation	527:539	The aim of this work was to assess the phenolic composition and the total antioxidant capacity (TAC) of MH, as well as its effects on cellular viability, proliferation, apoptosis and metabolism in lipopolysaccharide (LPS)-treated RAW 264.7 macrophages, highlighting the molecular mechanisms involved.
30189316	3	31	from	capacity	459:466	arg1	viability					516:524	cellular viability	507:524	cellular viability	507:524	The aim of this work was to assess the phenolic composition and the total antioxidant capacity (TAC) of MH, as well as its effects on cellular viability, proliferation, apoptosis and metabolism in lipopolysaccharide (LPS)-treated RAW 264.7 macrophages, highlighting the molecular mechanisms involved.
30189316	3	31	from	capacity	459:466	arg1	metabolism					556:565	metabolism	556:565	metabolism	556:565	The aim of this work was to assess the phenolic composition and the total antioxidant capacity (TAC) of MH, as well as its effects on cellular viability, proliferation, apoptosis and metabolism in lipopolysaccharide (LPS)-treated RAW 264.7 macrophages, highlighting the molecular mechanisms involved.
30189316	3	32	from	viability	516:524	arg1	macrophages					613:623	lipopolysaccharide (LPS)-treated RAW 264.7 macrophages	570:623	lipopolysaccharide (LPS)-treated RAW 264.7 macrophages	570:623	The aim of this work was to assess the phenolic composition and the total antioxidant capacity (TAC) of MH, as well as its effects on cellular viability, proliferation, apoptosis and metabolism in lipopolysaccharide (LPS)-treated RAW 264.7 macrophages, highlighting the molecular mechanisms involved.
30189316	7	33	theme	possible	1281:1288	arg1	mechanisms					1290:1299	the possible mechanisms	1277:1299	the possible mechanisms by which MH exerts its beneficial effects	1277:1341	In conclusion, one of the possible mechanisms by which MH exerts its beneficial effects could be to its capacity to improve cellular viability, promote proliferation and enhance energetic metabolism, by modulating the expression of several proteins involved in apoptosis, inflammation, metabolism and mitochondrial biogenesis.
30189316	3	34	theme	MH	477:478	arg1	effects					496:502	its effects	492:502	its effects on cellular viability, proliferation, apoptosis and metabolism in lipopolysaccharide (LPS)-treated RAW 264.7 macrophages	492:623	The aim of this work was to assess the phenolic composition and the total antioxidant capacity (TAC) of MH, as well as its effects on cellular viability, proliferation, apoptosis and metabolism in lipopolysaccharide (LPS)-treated RAW 264.7 macrophages, highlighting the molecular mechanisms involved.
30189316	3	34	theme	MH	477:478	arg1	capacity					459:466	the total antioxidant capacity	437:466	the total antioxidant capacity (TAC) of MH	437:478	The aim of this work was to assess the phenolic composition and the total antioxidant capacity (TAC) of MH, as well as its effects on cellular viability, proliferation, apoptosis and metabolism in lipopolysaccharide (LPS)-treated RAW 264.7 macrophages, highlighting the molecular mechanisms involved.
30189316	3	34	theme	MH	477:478	arg1	composition					421:431	the phenolic composition	408:431	the phenolic composition	408:431	The aim of this work was to assess the phenolic composition and the total antioxidant capacity (TAC) of MH, as well as its effects on cellular viability, proliferation, apoptosis and metabolism in lipopolysaccharide (LPS)-treated RAW 264.7 macrophages, highlighting the molecular mechanisms involved.
30189316	3	34	theme	MH	477:478	arg1	TAC					469:471	TAC	469:471	TAC	469:471	The aim of this work was to assess the phenolic composition and the total antioxidant capacity (TAC) of MH, as well as its effects on cellular viability, proliferation, apoptosis and metabolism in lipopolysaccharide (LPS)-treated RAW 264.7 macrophages, highlighting the molecular mechanisms involved.
30189316	3	35	from	proliferation	527:539	arg1	macrophages					613:623	lipopolysaccharide (LPS)-treated RAW 264.7 macrophages	570:623	lipopolysaccharide (LPS)-treated RAW 264.7 macrophages	570:623	The aim of this work was to assess the phenolic composition and the total antioxidant capacity (TAC) of MH, as well as its effects on cellular viability, proliferation, apoptosis and metabolism in lipopolysaccharide (LPS)-treated RAW 264.7 macrophages, highlighting the molecular mechanisms involved.
30189316	2	36	from	Australia	242:250	arg1	honey					231:235	a monofloral honey	218:235	a monofloral honey from Australia and New Zealand	218:266	Manuka honey (MH) is a monofloral honey from Australia and New Zealand, well-known for its healthy properties, such as antioxidant, antimicrobial and wound healing capacities.
30189316	2	36	from	Australia	242:250	arg1	honey					204:208	Manuka honey	197:208	Manuka honey (MH)	197:213	Manuka honey (MH) is a monofloral honey from Australia and New Zealand, well-known for its healthy properties, such as antioxidant, antimicrobial and wound healing capacities.
30189316	1	37	dep	Part	73:76	arg1	improvement					153:163	improvement	153:163	improvement of mitochondrial functionality	153:194	Part 1: Enhancement of cellular viability, regulation of cellular apoptosis and improvement of mitochondrial functionality.
30189316	1	37	dep	Part	73:76	arg1	regulation					116:125	regulation	116:125	regulation of cellular apoptosis	116:147	Part 1: Enhancement of cellular viability, regulation of cellular apoptosis and improvement of mitochondrial functionality.
30189316	1	37	dep	Part	73:76	arg1	Enhancement					81:91	Enhancement	81:91	Enhancement of cellular viability	81:113	Part 1: Enhancement of cellular viability, regulation of cellular apoptosis and improvement of mitochondrial functionality.
30189316	1	38	theme	functionality	182:194	arg1	improvement					153:163	improvement	153:163	improvement of mitochondrial functionality	153:194	Part 1: Enhancement of cellular viability, regulation of cellular apoptosis and improvement of mitochondrial functionality.
30189316	1	38	theme	functionality	182:194	arg1	regulation					116:125	regulation	116:125	regulation of cellular apoptosis	116:147	Part 1: Enhancement of cellular viability, regulation of cellular apoptosis and improvement of mitochondrial functionality.
30189316	1	38	theme	functionality	182:194	arg1	Enhancement					81:91	Enhancement	81:91	Enhancement of cellular viability	81:113	Part 1: Enhancement of cellular viability, regulation of cellular apoptosis and improvement of mitochondrial functionality.
30189316	0	39	theme	honey	29:33	arg1	effects					11:17	Protective effects	0:17	Protective effects of Manuka honey on LPS-treated RAW 264.7 macrophages	0:70	Protective effects of Manuka honey on LPS-treated RAW 264.7 macrophages.
30189316	7	40	theme	several	1487:1493	arg1	proteins					1495:1502	several proteins	1487:1502	several proteins involved in apoptosis, inflammation, metabolism and mitochondrial biogenesis	1487:1579	In conclusion, one of the possible mechanisms by which MH exerts its beneficial effects could be to its capacity to improve cellular viability, promote proliferation and enhance energetic metabolism, by modulating the expression of several proteins involved in apoptosis, inflammation, metabolism and mitochondrial biogenesis.
30189316	3	41	theme	phenolic	412:419	arg1	composition					421:431	the phenolic composition	408:431	the phenolic composition	408:431	The aim of this work was to assess the phenolic composition and the total antioxidant capacity (TAC) of MH, as well as its effects on cellular viability, proliferation, apoptosis and metabolism in lipopolysaccharide (LPS)-treated RAW 264.7 macrophages, highlighting the molecular mechanisms involved.
30189316	0	42	theme	RAW	50:52	arg1	macrophages					60:70	LPS-treated RAW 264.7 macrophages	38:70	LPS-treated RAW 264.7 macrophages	38:70	Protective effects of Manuka honey on LPS-treated RAW 264.7 macrophages.
30189316	7	43	theme	proteins	1495:1502	arg1	expression					1473:1482	the expression	1469:1482	the expression of several proteins involved in apoptosis, inflammation, metabolism and mitochondrial biogenesis	1469:1579	In conclusion, one of the possible mechanisms by which MH exerts its beneficial effects could be to its capacity to improve cellular viability, promote proliferation and enhance energetic metabolism, by modulating the expression of several proteins involved in apoptosis, inflammation, metabolism and mitochondrial biogenesis.
30189316	3	44	theme	lipopolysaccharide	570:587	arg1	macrophages					613:623	lipopolysaccharide (LPS)-treated RAW 264.7 macrophages	570:623	lipopolysaccharide (LPS)-treated RAW 264.7 macrophages	570:623	The aim of this work was to assess the phenolic composition and the total antioxidant capacity (TAC) of MH, as well as its effects on cellular viability, proliferation, apoptosis and metabolism in lipopolysaccharide (LPS)-treated RAW 264.7 macrophages, highlighting the molecular mechanisms involved.
30189316	3	45	theme	cellular	507:514	arg1	viability					516:524	cellular viability	507:524	cellular viability	507:524	The aim of this work was to assess the phenolic composition and the total antioxidant capacity (TAC) of MH, as well as its effects on cellular viability, proliferation, apoptosis and metabolism in lipopolysaccharide (LPS)-treated RAW 264.7 macrophages, highlighting the molecular mechanisms involved.
30189316	1	46	theme	cellular	96:103	arg1	viability					105:113	cellular viability	96:113	cellular viability	96:113	Part 1: Enhancement of cellular viability, regulation of cellular apoptosis and improvement of mitochondrial functionality.
30189316	0	47	theme	LPS-treated	38:48	arg1	macrophages					60:70	LPS-treated RAW 264.7 macrophages	38:70	LPS-treated RAW 264.7 macrophages	38:70	Protective effects of Manuka honey on LPS-treated RAW 264.7 macrophages.
30189316	3	48	theme	RAW	603:605	arg1	macrophages					613:623	lipopolysaccharide (LPS)-treated RAW 264.7 macrophages	570:623	lipopolysaccharide (LPS)-treated RAW 264.7 macrophages	570:623	The aim of this work was to assess the phenolic composition and the total antioxidant capacity (TAC) of MH, as well as its effects on cellular viability, proliferation, apoptosis and metabolism in lipopolysaccharide (LPS)-treated RAW 264.7 macrophages, highlighting the molecular mechanisms involved.
30189316	5	49	theme	dose-dependent	927:940	arg1	manner					942:947	a dose-dependent manner	925:947	a dose-dependent manner	925:947	In addition, MH was able to enhance cellular viability, decrease apoptosis, promote wound healing and attenuate inflammation in a dose-dependent manner, by reducing the expression of caspase 3, p-p38 and p-Erk1/2 proteins, in macrophages stressed with LPS.
30189316	3	50	from	composition	421:431	arg1	apoptosis					542:550	apoptosis	542:550	apoptosis	542:550	The aim of this work was to assess the phenolic composition and the total antioxidant capacity (TAC) of MH, as well as its effects on cellular viability, proliferation, apoptosis and metabolism in lipopolysaccharide (LPS)-treated RAW 264.7 macrophages, highlighting the molecular mechanisms involved.
30189316	3	50	from	composition	421:431	arg1	proliferation					527:539	proliferation	527:539	proliferation	527:539	The aim of this work was to assess the phenolic composition and the total antioxidant capacity (TAC) of MH, as well as its effects on cellular viability, proliferation, apoptosis and metabolism in lipopolysaccharide (LPS)-treated RAW 264.7 macrophages, highlighting the molecular mechanisms involved.
30189316	3	50	from	composition	421:431	arg1	viability					516:524	cellular viability	507:524	cellular viability	507:524	The aim of this work was to assess the phenolic composition and the total antioxidant capacity (TAC) of MH, as well as its effects on cellular viability, proliferation, apoptosis and metabolism in lipopolysaccharide (LPS)-treated RAW 264.7 macrophages, highlighting the molecular mechanisms involved.
30189316	3	50	from	composition	421:431	arg1	metabolism					556:565	metabolism	556:565	metabolism	556:565	The aim of this work was to assess the phenolic composition and the total antioxidant capacity (TAC) of MH, as well as its effects on cellular viability, proliferation, apoptosis and metabolism in lipopolysaccharide (LPS)-treated RAW 264.7 macrophages, highlighting the molecular mechanisms involved.
30189316	1	51	theme	viability	105:113	arg1	improvement					153:163	improvement	153:163	improvement of mitochondrial functionality	153:194	Part 1: Enhancement of cellular viability, regulation of cellular apoptosis and improvement of mitochondrial functionality.
30189316	1	51	theme	viability	105:113	arg1	regulation					116:125	regulation	116:125	regulation of cellular apoptosis	116:147	Part 1: Enhancement of cellular viability, regulation of cellular apoptosis and improvement of mitochondrial functionality.
30189316	1	51	theme	viability	105:113	arg1	Enhancement					81:91	Enhancement	81:91	Enhancement of cellular viability	81:113	Part 1: Enhancement of cellular viability, regulation of cellular apoptosis and improvement of mitochondrial functionality.
30189316	4	52	theme	gallic	721:726	arg1	acid					728:731	gallic acid	721:731	gallic acid	721:731	Up to 18 compounds were identified in MH, with gallic acid and quercetin as the major ones; MH showed also remarkable TAC.
30189316	6	53	theme	SIRT1	1170:1174	arg1	expression					1148:1157	the expression	1144:1157	the expression of p-AMPK, SIRT1 and PGC1α	1144:1184	In addition, it improved mitochondrial respiration and glycolytic activities, stimulating the expression of p-AMPK, SIRT1 and PGC1α, counteracting in this way the deleterious effects of LPS treatment.
30189316	7	54	theme	cellular	1379:1386	arg1	viability					1388:1396	cellular viability	1379:1396	cellular viability	1379:1396	In conclusion, one of the possible mechanisms by which MH exerts its beneficial effects could be to its capacity to improve cellular viability, promote proliferation and enhance energetic metabolism, by modulating the expression of several proteins involved in apoptosis, inflammation, metabolism and mitochondrial biogenesis.
30189316	5	55	theme	wound	881:885	arg1	healing					887:893	wound healing	881:893	wound healing	881:893	In addition, MH was able to enhance cellular viability, decrease apoptosis, promote wound healing and attenuate inflammation in a dose-dependent manner, by reducing the expression of caspase 3, p-p38 and p-Erk1/2 proteins, in macrophages stressed with LPS.
30189316	7	56	theme	mechanisms	1290:1299	arg1	one					1270:1272	one	1270:1272	one	1270:1272	In conclusion, one of the possible mechanisms by which MH exerts its beneficial effects could be to its capacity to improve cellular viability, promote proliferation and enhance energetic metabolism, by modulating the expression of several proteins involved in apoptosis, inflammation, metabolism and mitochondrial biogenesis.
30189316	7	56	theme	mechanisms	1290:1299	arg1	mechanisms					1290:1299	the possible mechanisms	1277:1299	the possible mechanisms by which MH exerts its beneficial effects	1277:1341	In conclusion, one of the possible mechanisms by which MH exerts its beneficial effects could be to its capacity to improve cellular viability, promote proliferation and enhance energetic metabolism, by modulating the expression of several proteins involved in apoptosis, inflammation, metabolism and mitochondrial biogenesis.
30189316	6	57	theme	PGC1α	1180:1184	arg1	expression					1148:1157	the expression	1144:1157	the expression of p-AMPK, SIRT1 and PGC1α	1144:1184	In addition, it improved mitochondrial respiration and glycolytic activities, stimulating the expression of p-AMPK, SIRT1 and PGC1α, counteracting in this way the deleterious effects of LPS treatment.
30189316	2	58	theme	Manuka	197:202	arg1	honey					231:235	a monofloral honey	218:235	a monofloral honey from Australia and New Zealand	218:266	Manuka honey (MH) is a monofloral honey from Australia and New Zealand, well-known for its healthy properties, such as antioxidant, antimicrobial and wound healing capacities.
30189316	2	58	theme	Manuka	197:202	arg1	MH					211:212	MH	211:212	MH	211:212	Manuka honey (MH) is a monofloral honey from Australia and New Zealand, well-known for its healthy properties, such as antioxidant, antimicrobial and wound healing capacities.
30189316	2	58	theme	Manuka	197:202	arg1	honey					204:208	Manuka honey	197:208	Manuka honey (MH)	197:213	Manuka honey (MH) is a monofloral honey from Australia and New Zealand, well-known for its healthy properties, such as antioxidant, antimicrobial and wound healing capacities.
30189316	6	59	theme	mitochondrial	1079:1091	arg1	respiration					1093:1103	mitochondrial respiration	1079:1103	mitochondrial respiration	1079:1103	In addition, it improved mitochondrial respiration and glycolytic activities, stimulating the expression of p-AMPK, SIRT1 and PGC1α, counteracting in this way the deleterious effects of LPS treatment.
30189316	3	60	theme	total	441:445	arg1	TAC					469:471	TAC	469:471	TAC	469:471	The aim of this work was to assess the phenolic composition and the total antioxidant capacity (TAC) of MH, as well as its effects on cellular viability, proliferation, apoptosis and metabolism in lipopolysaccharide (LPS)-treated RAW 264.7 macrophages, highlighting the molecular mechanisms involved.
30189316	3	60	theme	total	441:445	arg1	capacity					459:466	the total antioxidant capacity	437:466	the total antioxidant capacity (TAC) of MH	437:478	The aim of this work was to assess the phenolic composition and the total antioxidant capacity (TAC) of MH, as well as its effects on cellular viability, proliferation, apoptosis and metabolism in lipopolysaccharide (LPS)-treated RAW 264.7 macrophages, highlighting the molecular mechanisms involved.
30189316	3	61	from	effects	496:502	arg1	apoptosis					542:550	apoptosis	542:550	apoptosis	542:550	The aim of this work was to assess the phenolic composition and the total antioxidant capacity (TAC) of MH, as well as its effects on cellular viability, proliferation, apoptosis and metabolism in lipopolysaccharide (LPS)-treated RAW 264.7 macrophages, highlighting the molecular mechanisms involved.
30189316	3	61	from	effects	496:502	arg1	proliferation					527:539	proliferation	527:539	proliferation	527:539	The aim of this work was to assess the phenolic composition and the total antioxidant capacity (TAC) of MH, as well as its effects on cellular viability, proliferation, apoptosis and metabolism in lipopolysaccharide (LPS)-treated RAW 264.7 macrophages, highlighting the molecular mechanisms involved.
30189316	3	61	from	effects	496:502	arg1	viability					516:524	cellular viability	507:524	cellular viability	507:524	The aim of this work was to assess the phenolic composition and the total antioxidant capacity (TAC) of MH, as well as its effects on cellular viability, proliferation, apoptosis and metabolism in lipopolysaccharide (LPS)-treated RAW 264.7 macrophages, highlighting the molecular mechanisms involved.
30189316	3	61	from	effects	496:502	arg1	metabolism					556:565	metabolism	556:565	metabolism	556:565	The aim of this work was to assess the phenolic composition and the total antioxidant capacity (TAC) of MH, as well as its effects on cellular viability, proliferation, apoptosis and metabolism in lipopolysaccharide (LPS)-treated RAW 264.7 macrophages, highlighting the molecular mechanisms involved.
30189316	7	62	from	capacity	1359:1366	arg1	conclusion					1258:1267	conclusion	1258:1267	conclusion	1258:1267	In conclusion, one of the possible mechanisms by which MH exerts its beneficial effects could be to its capacity to improve cellular viability, promote proliferation and enhance energetic metabolism, by modulating the expression of several proteins involved in apoptosis, inflammation, metabolism and mitochondrial biogenesis.
30189316	3	63	theme	-treated	594:601	arg1	macrophages					613:623	lipopolysaccharide (LPS)-treated RAW 264.7 macrophages	570:623	lipopolysaccharide (LPS)-treated RAW 264.7 macrophages	570:623	The aim of this work was to assess the phenolic composition and the total antioxidant capacity (TAC) of MH, as well as its effects on cellular viability, proliferation, apoptosis and metabolism in lipopolysaccharide (LPS)-treated RAW 264.7 macrophages, highlighting the molecular mechanisms involved.
30189316	2	64	from	Zealand	260:266	arg1	honey					231:235	a monofloral honey	218:235	a monofloral honey from Australia and New Zealand	218:266	Manuka honey (MH) is a monofloral honey from Australia and New Zealand, well-known for its healthy properties, such as antioxidant, antimicrobial and wound healing capacities.
30189316	2	64	from	Zealand	260:266	arg1	honey					204:208	Manuka honey	197:208	Manuka honey (MH)	197:213	Manuka honey (MH) is a monofloral honey from Australia and New Zealand, well-known for its healthy properties, such as antioxidant, antimicrobial and wound healing capacities.
30189316	5	65	theme	p-Erk1/2	1001:1008	arg1	proteins					1010:1017	p-Erk1/2 proteins	1001:1017	p-Erk1/2 proteins	1001:1017	In addition, MH was able to enhance cellular viability, decrease apoptosis, promote wound healing and attenuate inflammation in a dose-dependent manner, by reducing the expression of caspase 3, p-p38 and p-Erk1/2 proteins, in macrophages stressed with LPS.
30189316	2	66	theme	monofloral	220:229	arg1	honey					231:235	a monofloral honey	218:235	a monofloral honey from Australia and New Zealand	218:266	Manuka honey (MH) is a monofloral honey from Australia and New Zealand, well-known for its healthy properties, such as antioxidant, antimicrobial and wound healing capacities.
30189316	2	66	theme	monofloral	220:229	arg1	honey					204:208	Manuka honey	197:208	Manuka honey (MH)	197:213	Manuka honey (MH) is a monofloral honey from Australia and New Zealand, well-known for its healthy properties, such as antioxidant, antimicrobial and wound healing capacities.
30189316	1	67	theme	cellular	130:137	arg1	apoptosis					139:147	cellular apoptosis	130:147	cellular apoptosis	130:147	Part 1: Enhancement of cellular viability, regulation of cellular apoptosis and improvement of mitochondrial functionality.
29561255	6	0	theme	Multilocus	514:523	arg1	analysis					534:541	Multilocus sequence analysis	514:541	Multilocus sequence analysis	514:541	Multilocus sequence analysis revealed low similarity of 91.93 % between MDB1-42T and Arthrobacter agilis NBRC 15319T.
29561255	7	1	theme	nucleotide	640:649	arg1	identity					651:658	Average nucleotide identity	632:658	Average nucleotide identity	632:658	Average nucleotide identity and digital DNA-DNA hybridization values between MDB1-42T and the most closely related strain, Arthrobacter agilis DSM 20550T, were 81.36 and 24.5 %, respectively.
29561255	4	2	theme	rRNA	310:313	arg1	sequences					320:328	16S rRNA gene sequences	306:328	16S rRNA gene sequences	306:328	Phylogenetic analysis based on 16S rRNA gene sequences revealed that MDB1-42T represented a member of the genus Arthrobacter.
29561255	12	3	theme	novel	1202:1206	arg1	species					1208:1214	a novel species	1200:1214	a novel species	1200:1214	On the basis of results obtained using a polyphasic approach, a novel species Arthrobacter ruber sp.
29561255	5	4	theme	highest	405:411	arg1	level					413:417	The highest level	401:417	The highest level of 16S rRNA gene sequence similarity (99.86 %)	401:464	The highest level of 16S rRNA gene sequence similarity (99.86 %) was found with Arthrobacter agilis NBRC 15319T.
29561255	13	5	theme	=CGMCC	1272:1277	arg1	113088T					1292:1298	=CGMCC 1.9772T=NBRC 113088T	1272:1298	=CGMCC 1.9772T=NBRC 113088T	1272:1298	nov. is proposed, with MDB1-42T (=CGMCC 1.9772T=NBRC 113088T) as the type strain.
29561255	13	5	theme	=CGMCC	1272:1277	arg1	MDB1-42T					1262:1269	MDB1-42T	1262:1269	MDB1-42T (=CGMCC 1.9772T=NBRC 113088T) as the type strain	1262:1318	nov. is proposed, with MDB1-42T (=CGMCC 1.9772T=NBRC 113088T) as the type strain.
29561255	4	6	theme	genus	381:385	arg1	Arthrobacter					387:398	the genus Arthrobacter	377:398	the genus Arthrobacter	377:398	Phylogenetic analysis based on 16S rRNA gene sequences revealed that MDB1-42T represented a member of the genus Arthrobacter.
29561255	2	7	attach	isolated	109:116	arg2	strain					78:83	A Gram-stain-positive strain	56:83	A Gram-stain-positive strain designated MDB1-42T	56:103	A Gram-stain-positive strain designated MDB1-42T was isolated from ice collected from Midui glacier in Tibet, PR China.
29561255	2	7	attach	isolated	109:116	arg1	ice					123:125	ice	123:125	ice collected from Midui glacier in Tibet, PR China	123:173	A Gram-stain-positive strain designated MDB1-42T was isolated from ice collected from Midui glacier in Tibet, PR China.
29561255	6	8	theme	Arthrobacter	599:610	arg1	15319T					624:629	Arthrobacter agilis NBRC 15319T	599:629	Arthrobacter agilis NBRC 15319T	599:629	Multilocus sequence analysis revealed low similarity of 91.93 % between MDB1-42T and Arthrobacter agilis NBRC 15319T.
29561255	8	9	theme	genomic	828:834	arg1	content					844:850	The genomic DNA G+C content	824:850	The genomic DNA G+C content	824:850	The genomic DNA G+C content was 69.0 mol%.
29561255	8	9	theme	genomic	828:834	arg1	%					864:864	69.0 mol%	856:864	69.0 mol%	856:864	The genomic DNA G+C content was 69.0 mol%.
29561255	9	10	theme	MDB1-42T	901:908	arg1	acids					892:896	The major cellular fatty acids	867:896	The major cellular fatty acids of MDB1-42T	867:908	The major cellular fatty acids of MDB1-42T were anteiso-C15 : 0 and anteiso-C17:0.
29561255	9	10	theme	MDB1-42T	901:908	arg1	anteiso-C15 					915:926	anteiso-C15 	915:926	anteiso-C15 	915:926	The major cellular fatty acids of MDB1-42T were anteiso-C15 : 0 and anteiso-C17:0.
29561255	1	11	theme	glacier	43:49	arg1	ice					51:53	glacier ice	43:53	glacier ice	43:53	nov., isolated from glacier ice.
29561255	8	12	theme	DNA	836:838	arg1	content					844:850	The genomic DNA G+C content	824:850	The genomic DNA G+C content	824:850	The genomic DNA G+C content was 69.0 mol%.
29561255	8	12	theme	DNA	836:838	arg1	%					864:864	69.0 mol%	856:864	69.0 mol%	856:864	The genomic DNA G+C content was 69.0 mol%.
29561255	5	13	theme	Arthrobacter	481:492	arg1	15319T					506:511	Arthrobacter agilis NBRC 15319T	481:511	Arthrobacter agilis NBRC 15319T	481:511	The highest level of 16S rRNA gene sequence similarity (99.86 %) was found with Arthrobacter agilis NBRC 15319T.
29561255	4	14	theme	Phylogenetic	275:286	arg1	analysis					288:295	Phylogenetic analysis	275:295	Phylogenetic analysis based on 16S rRNA gene sequences	275:328	Phylogenetic analysis based on 16S rRNA gene sequences revealed that MDB1-42T represented a member of the genus Arthrobacter.
29561255	4	15	theme	Arthrobacter	387:398	arg1	member					367:372	a member	365:372	a member of the genus Arthrobacter	365:398	Phylogenetic analysis based on 16S rRNA gene sequences revealed that MDB1-42T represented a member of the genus Arthrobacter.
29561255	3	16	theme	Strain	176:181	arg1	MDB1-42T					183:190	Strain MDB1-42T	176:190	Strain MDB1-42T	176:190	Strain MDB1-42T was catalase-positive, oxidase-negative and grew optimally at 25-28 °C and pH 7.0.
29561255	0	17	theme	Arthrobacter	0:11	arg1	sp					19:20	Arthrobacter ruber sp	0:20	Arthrobacter ruber sp.	0:21	Arthrobacter ruber sp.
29561255	0	18	theme	ruber	13:17	arg1	sp					19:20	Arthrobacter ruber sp	0:20	Arthrobacter ruber sp.	0:21	Arthrobacter ruber sp.
29561255	12	19	dep	sp	1235:1236	arg1	results					1154:1160	results	1154:1160	results obtained using a polyphasic approach	1154:1197	On the basis of results obtained using a polyphasic approach, a novel species Arthrobacter ruber sp.
29561255	12	19	dep	sp	1235:1236	arg1	species					1208:1214	a novel species	1200:1214	a novel species	1200:1214	On the basis of results obtained using a polyphasic approach, a novel species Arthrobacter ruber sp.
29561255	8	20	theme	69.0	856:859	arg1	mol					861:863	mol	861:863	mol	861:863	The genomic DNA G+C content was 69.0 mol%.
29561255	6	21	theme	agilis	612:617	arg1	15319T					624:629	Arthrobacter agilis NBRC 15319T	599:629	Arthrobacter agilis NBRC 15319T	599:629	Multilocus sequence analysis revealed low similarity of 91.93 % between MDB1-42T and Arthrobacter agilis NBRC 15319T.
29561255	3	22	dep	catalase-positive	196:212	arg1	oxidase-negative					215:230	oxidase-negative	215:230	oxidase-negative	215:230	Strain MDB1-42T was catalase-positive, oxidase-negative and grew optimally at 25-28 °C and pH 7.0.
29561255	10	23	theme	unidentified	1044:1055	arg1	glycolipid					1057:1066	one unidentified glycolipid	1040:1066	one unidentified glycolipid	1040:1066	The polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, one unidentified glycolipid and one unidentified lipid.
29561255	5	24	theme	similarity	445:454	arg1	level					413:417	The highest level	401:417	The highest level of 16S rRNA gene sequence similarity (99.86 %)	401:464	The highest level of 16S rRNA gene sequence similarity (99.86 %) was found with Arthrobacter agilis NBRC 15319T.
29561255	6	25	theme	%	576:576	arg1	similarity					556:565	low similarity	552:565	low similarity of 91.93 % between MDB1-42T and Arthrobacter agilis NBRC 15319T	552:629	Multilocus sequence analysis revealed low similarity of 91.93 % between MDB1-42T and Arthrobacter agilis NBRC 15319T.
29561255	12	26	dep	results	1154:1160	arg1	the					1141:1143	the	1141:1143	the	1141:1143	On the basis of results obtained using a polyphasic approach, a novel species Arthrobacter ruber sp.
29561255	12	26	dep	results	1154:1160	arg1	basis					1145:1149	basis	1145:1149	basis	1145:1149	On the basis of results obtained using a polyphasic approach, a novel species Arthrobacter ruber sp.
29561255	2	27	theme	PR	166:167	arg1	Tibet					159:163	Tibet	159:163	Tibet	159:163	A Gram-stain-positive strain designated MDB1-42T was isolated from ice collected from Midui glacier in Tibet, PR China.
29561255	2	27	theme	PR	166:167	arg1	China					169:173	PR China	166:173	PR China	166:173	A Gram-stain-positive strain designated MDB1-42T was isolated from ice collected from Midui glacier in Tibet, PR China.
29561255	12	28	theme	polyphasic	1179:1188	arg1	approach					1190:1197	a polyphasic approach	1177:1197	a polyphasic approach	1177:1197	On the basis of results obtained using a polyphasic approach, a novel species Arthrobacter ruber sp.
29561255	4	29	theme	gene	315:318	arg1	sequences					320:328	16S rRNA gene sequences	306:328	16S rRNA gene sequences	306:328	Phylogenetic analysis based on 16S rRNA gene sequences revealed that MDB1-42T represented a member of the genus Arthrobacter.
29561255	8	30	theme	G+C	840:842	arg1	content					844:850	The genomic DNA G+C content	824:850	The genomic DNA G+C content	824:850	The genomic DNA G+C content was 69.0 mol%.
29561255	8	30	theme	G+C	840:842	arg1	%					864:864	69.0 mol%	856:864	69.0 mol%	856:864	The genomic DNA G+C content was 69.0 mol%.
29561255	11	31	theme	predominant	1100:1110	arg1	MK-9					1128:1131	MK-9	1128:1131	MK-9	1128:1131	The predominant menaquinone was MK-9(H2).
29561255	11	31	theme	predominant	1100:1110	arg1	menaquinone					1112:1122	The predominant menaquinone	1096:1122	The predominant menaquinone	1096:1122	The predominant menaquinone was MK-9(H2).
29561255	13	32	theme	1.9772T=NBRC	1279:1290	arg1	113088T					1292:1298	=CGMCC 1.9772T=NBRC 113088T	1272:1298	=CGMCC 1.9772T=NBRC 113088T	1272:1298	nov. is proposed, with MDB1-42T (=CGMCC 1.9772T=NBRC 113088T) as the type strain.
29561255	13	32	theme	1.9772T=NBRC	1279:1290	arg1	MDB1-42T					1262:1269	MDB1-42T	1262:1269	MDB1-42T (=CGMCC 1.9772T=NBRC 113088T) as the type strain	1262:1318	nov. is proposed, with MDB1-42T (=CGMCC 1.9772T=NBRC 113088T) as the type strain.
29561255	7	33	theme	hybridization	680:692	arg1	values					694:699	Average nucleotide identity and digital DNA-DNA hybridization values	632:699	Average nucleotide identity and digital DNA-DNA hybridization values between MDB1-42T and the most closely related strain, Arthrobacter agilis DSM 20550T,	632:785	Average nucleotide identity and digital DNA-DNA hybridization values between MDB1-42T and the most closely related strain, Arthrobacter agilis DSM 20550T, were 81.36 and 24.5 %, respectively.
29561255	13	34	theme	type	1308:1311	arg1	strain					1313:1318	the type strain	1304:1318	the type strain	1304:1318	nov. is proposed, with MDB1-42T (=CGMCC 1.9772T=NBRC 113088T) as the type strain.
29561255	7	35	theme	DSM	775:777	arg1	20550T					779:784	Arthrobacter agilis DSM 20550T	755:784	Arthrobacter agilis DSM 20550T	755:784	Average nucleotide identity and digital DNA-DNA hybridization values between MDB1-42T and the most closely related strain, Arthrobacter agilis DSM 20550T, were 81.36 and 24.5 %, respectively.
29561255	7	35	theme	DSM	775:777	arg1	strain					747:752	the most closely related strain	722:752	the most closely related strain	722:752	Average nucleotide identity and digital DNA-DNA hybridization values between MDB1-42T and the most closely related strain, Arthrobacter agilis DSM 20550T, were 81.36 and 24.5 %, respectively.
29561255	6	36	theme	low	552:554	arg1	similarity					556:565	low similarity	552:565	low similarity of 91.93 % between MDB1-42T and Arthrobacter agilis NBRC 15319T	552:629	Multilocus sequence analysis revealed low similarity of 91.93 % between MDB1-42T and Arthrobacter agilis NBRC 15319T.
29561255	9	37	dep	anteiso-C15 	915:926	arg1	anteiso-C17:0					935:947	anteiso-C17:0	935:947	anteiso-C17:0	935:947	The major cellular fatty acids of MDB1-42T were anteiso-C15 : 0 and anteiso-C17:0.
29561255	9	37	dep	anteiso-C15 	915:926	arg1	 0					928:929	 0	928:929	 0	928:929	The major cellular fatty acids of MDB1-42T were anteiso-C15 : 0 and anteiso-C17:0.
29561255	9	38	theme	fatty	886:890	arg1	acids					892:896	The major cellular fatty acids	867:896	The major cellular fatty acids of MDB1-42T	867:908	The major cellular fatty acids of MDB1-42T were anteiso-C15 : 0 and anteiso-C17:0.
29561255	9	38	theme	fatty	886:890	arg1	anteiso-C15 					915:926	anteiso-C15 	915:926	anteiso-C15 	915:926	The major cellular fatty acids of MDB1-42T were anteiso-C15 : 0 and anteiso-C17:0.
29561255	12	39	theme	Arthrobacter	1216:1227	arg1	sp					1235:1236	Arthrobacter ruber sp	1216:1236	Arthrobacter ruber sp	1216:1236	On the basis of results obtained using a polyphasic approach, a novel species Arthrobacter ruber sp.
29561255	7	40	theme	agilis	768:773	arg1	DSM					775:777	Arthrobacter agilis DSM	755:777	Arthrobacter agilis DSM 20550T	755:784	Average nucleotide identity and digital DNA-DNA hybridization values between MDB1-42T and the most closely related strain, Arthrobacter agilis DSM 20550T, were 81.36 and 24.5 %, respectively.
29561255	10	41	theme	unidentified	1076:1087	arg1	lipid					1089:1093	one unidentified lipid	1072:1093	one unidentified lipid	1072:1093	The polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, one unidentified glycolipid and one unidentified lipid.
29561255	7	42	theme	digital	664:670	arg1	hybridization					680:692	digital DNA-DNA hybridization	664:692	digital DNA-DNA hybridization	664:692	Average nucleotide identity and digital DNA-DNA hybridization values between MDB1-42T and the most closely related strain, Arthrobacter agilis DSM 20550T, were 81.36 and 24.5 %, respectively.
29561255	5	43	theme	16S	422:424	arg1	similarity					445:454	16S rRNA gene sequence similarity	422:454	16S rRNA gene sequence similarity (99.86 %)	422:464	The highest level of 16S rRNA gene sequence similarity (99.86 %) was found with Arthrobacter agilis NBRC 15319T.
29561255	5	43	theme	16S	422:424	arg1	%					463:463	99.86 %	457:463	99.86 %	457:463	The highest level of 16S rRNA gene sequence similarity (99.86 %) was found with Arthrobacter agilis NBRC 15319T.
29561255	2	44	theme	Gram-stain-positive	58:76	arg1	strain					78:83	A Gram-stain-positive strain	56:83	A Gram-stain-positive strain designated MDB1-42T	56:103	A Gram-stain-positive strain designated MDB1-42T was isolated from ice collected from Midui glacier in Tibet, PR China.
29561255	7	45	theme	DNA-DNA	672:678	arg1	hybridization					680:692	digital DNA-DNA hybridization	664:692	digital DNA-DNA hybridization	664:692	Average nucleotide identity and digital DNA-DNA hybridization values between MDB1-42T and the most closely related strain, Arthrobacter agilis DSM 20550T, were 81.36 and 24.5 %, respectively.
29561255	5	46	theme	rRNA	426:429	arg1	similarity					445:454	16S rRNA gene sequence similarity	422:454	16S rRNA gene sequence similarity (99.86 %)	422:464	The highest level of 16S rRNA gene sequence similarity (99.86 %) was found with Arthrobacter agilis NBRC 15319T.
29561255	5	46	theme	rRNA	426:429	arg1	%					463:463	99.86 %	457:463	99.86 %	457:463	The highest level of 16S rRNA gene sequence similarity (99.86 %) was found with Arthrobacter agilis NBRC 15319T.
29561255	9	47	theme	major	871:875	arg1	acids					892:896	The major cellular fatty acids	867:896	The major cellular fatty acids of MDB1-42T	867:908	The major cellular fatty acids of MDB1-42T were anteiso-C15 : 0 and anteiso-C17:0.
29561255	9	47	theme	major	871:875	arg1	anteiso-C15 					915:926	anteiso-C15 	915:926	anteiso-C15 	915:926	The major cellular fatty acids of MDB1-42T were anteiso-C15 : 0 and anteiso-C17:0.
29561255	7	48	theme	identity	651:658	arg1	values					694:699	Average nucleotide identity and digital DNA-DNA hybridization values	632:699	Average nucleotide identity and digital DNA-DNA hybridization values between MDB1-42T and the most closely related strain, Arthrobacter agilis DSM 20550T,	632:785	Average nucleotide identity and digital DNA-DNA hybridization values between MDB1-42T and the most closely related strain, Arthrobacter agilis DSM 20550T, were 81.36 and 24.5 %, respectively.
29561255	4	49	theme	16S	306:308	arg1	sequences					320:328	16S rRNA gene sequences	306:328	16S rRNA gene sequences	306:328	Phylogenetic analysis based on 16S rRNA gene sequences revealed that MDB1-42T represented a member of the genus Arthrobacter.
29561255	12	50	theme	ruber	1229:1233	arg1	sp					1235:1236	Arthrobacter ruber sp	1216:1236	Arthrobacter ruber sp	1216:1236	On the basis of results obtained using a polyphasic approach, a novel species Arthrobacter ruber sp.
29561255	5	51	theme	gene	431:434	arg1	similarity					445:454	16S rRNA gene sequence similarity	422:454	16S rRNA gene sequence similarity (99.86 %)	422:464	The highest level of 16S rRNA gene sequence similarity (99.86 %) was found with Arthrobacter agilis NBRC 15319T.
29561255	5	51	theme	gene	431:434	arg1	%					463:463	99.86 %	457:463	99.86 %	457:463	The highest level of 16S rRNA gene sequence similarity (99.86 %) was found with Arthrobacter agilis NBRC 15319T.
29561255	7	52	theme	Arthrobacter	755:766	arg1	DSM					775:777	Arthrobacter agilis DSM	755:777	Arthrobacter agilis DSM 20550T	755:784	Average nucleotide identity and digital DNA-DNA hybridization values between MDB1-42T and the most closely related strain, Arthrobacter agilis DSM 20550T, were 81.36 and 24.5 %, respectively.
29561255	9	53	theme	cellular	877:884	arg1	acids					892:896	The major cellular fatty acids	867:896	The major cellular fatty acids of MDB1-42T	867:908	The major cellular fatty acids of MDB1-42T were anteiso-C15 : 0 and anteiso-C17:0.
29561255	9	53	theme	cellular	877:884	arg1	anteiso-C15 					915:926	anteiso-C15 	915:926	anteiso-C15 	915:926	The major cellular fatty acids of MDB1-42T were anteiso-C15 : 0 and anteiso-C17:0.
29561255	10	54	theme	polar	954:958	arg1	phosphatidylglycerol					972:991	phosphatidylglycerol	972:991	phosphatidylglycerol	972:991	The polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, one unidentified glycolipid and one unidentified lipid.
29561255	10	54	theme	polar	954:958	arg1	lipids					960:965	The polar lipids	950:965	The polar lipids	950:965	The polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, one unidentified glycolipid and one unidentified lipid.
29561255	5	55	theme	agilis	494:499	arg1	15319T					506:511	Arthrobacter agilis NBRC 15319T	481:511	Arthrobacter agilis NBRC 15319T	481:511	The highest level of 16S rRNA gene sequence similarity (99.86 %) was found with Arthrobacter agilis NBRC 15319T.
29561255	5	56	theme	sequence	436:443	arg1	similarity					445:454	16S rRNA gene sequence similarity	422:454	16S rRNA gene sequence similarity (99.86 %)	422:464	The highest level of 16S rRNA gene sequence similarity (99.86 %) was found with Arthrobacter agilis NBRC 15319T.
29561255	5	56	theme	sequence	436:443	arg1	%					463:463	99.86 %	457:463	99.86 %	457:463	The highest level of 16S rRNA gene sequence similarity (99.86 %) was found with Arthrobacter agilis NBRC 15319T.
29561255	7	57	theme	related	739:745	arg1	20550T					779:784	Arthrobacter agilis DSM 20550T	755:784	Arthrobacter agilis DSM 20550T	755:784	Average nucleotide identity and digital DNA-DNA hybridization values between MDB1-42T and the most closely related strain, Arthrobacter agilis DSM 20550T, were 81.36 and 24.5 %, respectively.
29561255	7	57	theme	related	739:745	arg1	strain					747:752	the most closely related strain	722:752	the most closely related strain	722:752	Average nucleotide identity and digital DNA-DNA hybridization values between MDB1-42T and the most closely related strain, Arthrobacter agilis DSM 20550T, were 81.36 and 24.5 %, respectively.
29561255	6	58	theme	sequence	525:532	arg1	analysis					534:541	Multilocus sequence analysis	514:541	Multilocus sequence analysis	514:541	Multilocus sequence analysis revealed low similarity of 91.93 % between MDB1-42T and Arthrobacter agilis NBRC 15319T.
29561255	7	59	theme	Average	632:638	arg1	identity					651:658	Average nucleotide identity	632:658	Average nucleotide identity	632:658	Average nucleotide identity and digital DNA-DNA hybridization values between MDB1-42T and the most closely related strain, Arthrobacter agilis DSM 20550T, were 81.36 and 24.5 %, respectively.
29561255	5	60	theme	NBRC	501:504	arg1	15319T					506:511	Arthrobacter agilis NBRC 15319T	481:511	Arthrobacter agilis NBRC 15319T	481:511	The highest level of 16S rRNA gene sequence similarity (99.86 %) was found with Arthrobacter agilis NBRC 15319T.
29561255	6	61	theme	NBRC	619:622	arg1	15319T					624:629	Arthrobacter agilis NBRC 15319T	599:629	Arthrobacter agilis NBRC 15319T	599:629	Multilocus sequence analysis revealed low similarity of 91.93 % between MDB1-42T and Arthrobacter agilis NBRC 15319T.
29879404	7	0	theme	PCB	1252:1254	arg1	exposure					1256:1263	PCB exposure	1252:1263	PCB exposure	1252:1263	Across both basal and activated inflammatory states, PCB exposure caused greater expression of a subset of inflammatory genes in the hypothalamus and lower expression of genes involved in dopamine, serotonin, and opioid systems compared to oil controls.
29879404	7	1	theme	oil	1439:1441	arg1	controls					1443:1450	oil controls	1439:1450	oil controls	1439:1450	Across both basal and activated inflammatory states, PCB exposure caused greater expression of a subset of inflammatory genes in the hypothalamus and lower expression of genes involved in dopamine, serotonin, and opioid systems compared to oil controls.
29879404	10	2	theme	disruption	1770:1779	arg1	consequences					1742:1753	the potential long-term consequences	1718:1753	the potential long-term consequences of neuroimmune disruption	1718:1779	Given the potential long-term consequences of neuroimmune disruption, our findings demonstrate the need for further research.
29879404	6	3	theme	tissue	1180:1185	arg1	collection					1187:1196	tissue collection	1180:1196	tissue collection	1180:1196	One day old male and female offspring were treated with an inflammatory challenge (lipopolysaccharide, LPS, 50 μg/kg, sc) or saline vehicle control approximately 3.5 h prior to tissue collection.
29879404	4	4	theme	gestational	667:677	arg1	exposure					683:690	gestational PCB exposure	667:690	gestational PCB exposure	667:690	The current study used Sprague-Dawley rats to assess whether gestational PCB exposure altered hypothalamic gene expression and serum cytokine concentration in neonatal animals given an immune challenge.
29879404	3	5	theme	neuromodulatory	528:542	arg1	roles					544:548	neuromodulatory roles	528:548	neuromodulatory roles	528:548	This is an important gap in knowledge, as molecules typically associated with neuroinflammation also serve neuromodulatory roles and interact with hormones in normal brain development.
29879404	9	6	theme	effects	1685:1691	arg1	Many					1671:1674	Many	1671:1674	Many	1671:1674	Many of these effects were sex specific.
29879404	9	6	theme	effects	1685:1691	arg1	effects					1685:1691	these effects	1679:1691	these effects	1679:1691	Many of these effects were sex specific.
29879404	7	7	theme	opioid	1412:1417	arg1	systems					1419:1425	dopamine, serotonin, and opioid systems	1387:1425	dopamine, serotonin, and opioid systems	1387:1425	Across both basal and activated inflammatory states, PCB exposure caused greater expression of a subset of inflammatory genes in the hypothalamus and lower expression of genes involved in dopamine, serotonin, and opioid systems compared to oil controls.
29879404	6	8	dep	prior	1171:1175	arg1	3.5 h					1165:1169	3.5 h	1165:1169	3.5 h	1165:1169	One day old male and female offspring were treated with an inflammatory challenge (lipopolysaccharide, LPS, 50 μg/kg, sc) or saline vehicle control approximately 3.5 h prior to tissue collection.
29879404	4	9	used	used	624:627	arg2	study					618:622	The current study	606:622	The current study	606:622	The current study used Sprague-Dawley rats to assess whether gestational PCB exposure altered hypothalamic gene expression and serum cytokine concentration in neonatal animals given an immune challenge.
29879404	10	10	theme	further	1820:1826	arg1	research					1828:1835	further research	1820:1835	further research	1820:1835	Given the potential long-term consequences of neuroimmune disruption, our findings demonstrate the need for further research.
29879404	5	11	contain	containing	830:839	arg1	wafers					823:828	wafers	823:828	wafers containing a mixture of PCBs at an environmentally relevant dose and composition (20 μg/kg, 1:1:1 Aroclor 1242:1248:1254) or oil vehicle control	823:973	Dams were fed wafers containing a mixture of PCBs at an environmentally relevant dose and composition (20 μg/kg, 1:1:1 Aroclor 1242:1248:1254) or oil vehicle control throughout their pregnancy.
29879404	5	11	contain	containing	830:839	arg2	mixture					843:849	a mixture	841:849	a mixture of PCBs	841:857	Dams were fed wafers containing a mixture of PCBs at an environmentally relevant dose and composition (20 μg/kg, 1:1:1 Aroclor 1242:1248:1254) or oil vehicle control throughout their pregnancy.
29879404	4	12	theme	serum	733:737	arg1	concentration					748:760	serum cytokine concentration	733:760	serum cytokine concentration	733:760	The current study used Sprague-Dawley rats to assess whether gestational PCB exposure altered hypothalamic gene expression and serum cytokine concentration in neonatal animals given an immune challenge.
29879404	3	13	theme	brain	587:591	arg1	development					593:603	normal brain development	580:603	normal brain development	580:603	This is an important gap in knowledge, as molecules typically associated with neuroinflammation also serve neuromodulatory roles and interact with hormones in normal brain development.
29879404	1	14	from	environment	204:214	arg1	ubiquitous					186:195	ubiquitous	186:195	ubiquitous	186:195	Polychlorinated biphenyls (PCBs) are ubiquitous in the environment and exposure to them is associated with immune, endocrine and neural dysfunction.
29879404	0	15	from	systems	123:129	arg1	differences					4:14	Sex differences	0:14	Sex differences in effects of gestational polychlorinated biphenyl exposure on hypothalamic neuroimmune and neuromodulator systems in neonatal rats.	0:147	Sex differences in effects of gestational polychlorinated biphenyl exposure on hypothalamic neuroimmune and neuromodulator systems in neonatal rats.
29879404	0	15	from	systems	123:129	arg1	rats					143:146	neonatal rats	134:146	neonatal rats	134:146	Sex differences in effects of gestational polychlorinated biphenyl exposure on hypothalamic neuroimmune and neuromodulator systems in neonatal rats.
29879404	7	16	theme	genes	1369:1373	arg1	expression					1355:1364	lower expression	1349:1364	lower expression of genes involved in dopamine, serotonin, and opioid systems compared to oil controls	1349:1450	Across both basal and activated inflammatory states, PCB exposure caused greater expression of a subset of inflammatory genes in the hypothalamus and lower expression of genes involved in dopamine, serotonin, and opioid systems compared to oil controls.
29879404	7	16	theme	genes	1369:1373	arg1	expression					1280:1289	greater expression	1272:1289	greater expression of a subset of inflammatory genes in the hypothalamus	1272:1343	Across both basal and activated inflammatory states, PCB exposure caused greater expression of a subset of inflammatory genes in the hypothalamus and lower expression of genes involved in dopamine, serotonin, and opioid systems compared to oil controls.
29879404	1	17	theme	Polychlorinated	149:163	arg1	PCBs					176:179	PCBs	176:179	PCBs	176:179	Polychlorinated biphenyls (PCBs) are ubiquitous in the environment and exposure to them is associated with immune, endocrine and neural dysfunction.
29879404	1	17	theme	Polychlorinated	149:163	arg1	biphenyls					165:173	Polychlorinated biphenyls	149:173	Polychlorinated biphenyls (PCBs)	149:180	Polychlorinated biphenyls (PCBs) are ubiquitous in the environment and exposure to them is associated with immune, endocrine and neural dysfunction.
29879404	2	18	from	Effects	298:304	arg1	immunity					334:341	immunity	334:341	immunity	334:341	Effects of PCBs on inflammation and immunity are best described in spleen and blood, with fewer studies on neural tissues.
29879404	2	18	from	Effects	298:304	arg1	inflammation					317:328	inflammation	317:328	inflammation	317:328	Effects of PCBs on inflammation and immunity are best described in spleen and blood, with fewer studies on neural tissues.
29879404	4	19	theme	hypothalamic	700:711	arg1	expression					718:727	hypothalamic gene expression	700:727	hypothalamic gene expression	700:727	The current study used Sprague-Dawley rats to assess whether gestational PCB exposure altered hypothalamic gene expression and serum cytokine concentration in neonatal animals given an immune challenge.
29879404	0	20	theme	exposure	67:74	arg1	effects					19:25	effects	19:25	effects of gestational polychlorinated biphenyl exposure on hypothalamic neuroimmune and neuromodulator systems in neonatal rats	19:146	Sex differences in effects of gestational polychlorinated biphenyl exposure on hypothalamic neuroimmune and neuromodulator systems in neonatal rats.
29879404	6	21	theme	female	1024:1029	arg1	offspring					1031:1039	One day old male and female offspring	1003:1039	One day old male and female offspring	1003:1039	One day old male and female offspring were treated with an inflammatory challenge (lipopolysaccharide, LPS, 50 μg/kg, sc) or saline vehicle control approximately 3.5 h prior to tissue collection.
29879404	0	22	theme	hypothalamic	79:90	arg1	neuroimmune					92:102	hypothalamic neuroimmune and neuromodulator systems	79:129	neuroimmune	92:102	Sex differences in effects of gestational polychlorinated biphenyl exposure on hypothalamic neuroimmune and neuromodulator systems in neonatal rats.
29879404	5	23	theme	1:1:1	922:926	arg1	20 μg/kg					912:919	20 μg/kg	912:919	20 μg/kg	912:919	Dams were fed wafers containing a mixture of PCBs at an environmentally relevant dose and composition (20 μg/kg, 1:1:1 Aroclor 1242:1248:1254) or oil vehicle control throughout their pregnancy.
29879404	5	23	theme	1:1:1	922:926	arg1	Aroclor					928:934	1:1:1 Aroclor 1242:1248:1254	922:949	1:1:1 Aroclor 1242:1248:1254	922:949	Dams were fed wafers containing a mixture of PCBs at an environmentally relevant dose and composition (20 μg/kg, 1:1:1 Aroclor 1242:1248:1254) or oil vehicle control throughout their pregnancy.
29879404	6	24	theme	male	1015:1018	arg1	offspring					1031:1039	One day old male and female offspring	1003:1039	One day old male and female offspring	1003:1039	One day old male and female offspring were treated with an inflammatory challenge (lipopolysaccharide, LPS, 50 μg/kg, sc) or saline vehicle control approximately 3.5 h prior to tissue collection.
29879404	4	25	theme	immune	791:796	arg1	challenge					798:806	an immune challenge	788:806	an immune challenge	788:806	The current study used Sprague-Dawley rats to assess whether gestational PCB exposure altered hypothalamic gene expression and serum cytokine concentration in neonatal animals given an immune challenge.
29879404	7	26	from	expression	1355:1364	arg1	hypothalamus					1332:1343	the hypothalamus	1328:1343	the hypothalamus	1328:1343	Across both basal and activated inflammatory states, PCB exposure caused greater expression of a subset of inflammatory genes in the hypothalamus and lower expression of genes involved in dopamine, serotonin, and opioid systems compared to oil controls.
29879404	6	27	theme	day	1007:1009	arg1	offspring					1031:1039	One day old male and female offspring	1003:1039	One day old male and female offspring	1003:1039	One day old male and female offspring were treated with an inflammatory challenge (lipopolysaccharide, LPS, 50 μg/kg, sc) or saline vehicle control approximately 3.5 h prior to tissue collection.
29879404	6	28	dep	challenge	1075:1083	arg1	lipopolysaccharide					1086:1103	lipopolysaccharide	1086:1103	lipopolysaccharide	1086:1103	One day old male and female offspring were treated with an inflammatory challenge (lipopolysaccharide, LPS, 50 μg/kg, sc) or saline vehicle control approximately 3.5 h prior to tissue collection.
29879404	6	28	dep	challenge	1075:1083	arg1	prior					1171:1175	prior	1171:1175	prior	1171:1175	One day old male and female offspring were treated with an inflammatory challenge (lipopolysaccharide, LPS, 50 μg/kg, sc) or saline vehicle control approximately 3.5 h prior to tissue collection.
29879404	6	28	dep	challenge	1075:1083	arg1	50 μg/kg					1111:1118	50 μg/kg	1111:1118	50 μg/kg	1111:1118	One day old male and female offspring were treated with an inflammatory challenge (lipopolysaccharide, LPS, 50 μg/kg, sc) or saline vehicle control approximately 3.5 h prior to tissue collection.
29879404	6	28	dep	challenge	1075:1083	arg1	sc					1121:1122	sc	1121:1122	sc	1121:1122	One day old male and female offspring were treated with an inflammatory challenge (lipopolysaccharide, LPS, 50 μg/kg, sc) or saline vehicle control approximately 3.5 h prior to tissue collection.
29879404	6	28	dep	challenge	1075:1083	arg1	LPS					1106:1108	LPS	1106:1108	LPS	1106:1108	One day old male and female offspring were treated with an inflammatory challenge (lipopolysaccharide, LPS, 50 μg/kg, sc) or saline vehicle control approximately 3.5 h prior to tissue collection.
29879404	2	29	theme	fewer	388:392	arg1	studies					394:400	fewer studies	388:400	fewer studies on neural tissues	388:418	Effects of PCBs on inflammation and immunity are best described in spleen and blood, with fewer studies on neural tissues.
29879404	7	30	theme	lower	1349:1353	arg1	expression					1355:1364	lower expression	1349:1364	lower expression of genes involved in dopamine, serotonin, and opioid systems compared to oil controls	1349:1450	Across both basal and activated inflammatory states, PCB exposure caused greater expression of a subset of inflammatory genes in the hypothalamus and lower expression of genes involved in dopamine, serotonin, and opioid systems compared to oil controls.
29879404	4	31	theme	neonatal	765:772	arg1	animals					774:780	neonatal animals	765:780	neonatal animals given an immune challenge	765:806	The current study used Sprague-Dawley rats to assess whether gestational PCB exposure altered hypothalamic gene expression and serum cytokine concentration in neonatal animals given an immune challenge.
29879404	0	32	theme	Sex	0:2	arg1	differences					4:14	Sex differences	0:14	Sex differences in effects of gestational polychlorinated biphenyl exposure on hypothalamic neuroimmune and neuromodulator systems in neonatal rats.	0:147	Sex differences in effects of gestational polychlorinated biphenyl exposure on hypothalamic neuroimmune and neuromodulator systems in neonatal rats.
29879404	1	33	theme	neural	278:283	arg1	dysfunction					285:295	immune, endocrine and neural dysfunction	256:295	immune, endocrine and neural dysfunction	256:295	Polychlorinated biphenyls (PCBs) are ubiquitous in the environment and exposure to them is associated with immune, endocrine and neural dysfunction.
29879404	8	34	theme	Esr2	1551:1554	arg1	expression					1569:1578	Esr2 hypothalamic expression	1551:1578	Esr2 hypothalamic expression	1551:1578	PCB exposure also altered reactions to inflammatory challenge: it reversed the normal decrease in Esr2 hypothalamic expression and induced an abnormal increase in IL-1b and IL-6 serum concentration in response to LPS.
29879404	6	35	theme	inflammatory	1062:1073	arg1	challenge					1075:1083	an inflammatory challenge	1059:1083	an inflammatory challenge	1059:1083	One day old male and female offspring were treated with an inflammatory challenge (lipopolysaccharide, LPS, 50 μg/kg, sc) or saline vehicle control approximately 3.5 h prior to tissue collection.
29879404	3	36	theme	important	432:440	arg1	gap					442:444	an important gap	429:444	an important gap in knowledge	429:457	This is an important gap in knowledge, as molecules typically associated with neuroinflammation also serve neuromodulatory roles and interact with hormones in normal brain development.
29879404	3	36	theme	important	432:440	arg1	This					421:424	This	421:424	This	421:424	This is an important gap in knowledge, as molecules typically associated with neuroinflammation also serve neuromodulatory roles and interact with hormones in normal brain development.
29879404	8	37	from	decrease	1539:1546	arg1	expression					1569:1578	Esr2 hypothalamic expression	1551:1578	Esr2 hypothalamic expression	1551:1578	PCB exposure also altered reactions to inflammatory challenge: it reversed the normal decrease in Esr2 hypothalamic expression and induced an abnormal increase in IL-1b and IL-6 serum concentration in response to LPS.
29879404	7	38	theme	inflammatory	1306:1317	arg1	genes					1319:1323	inflammatory genes	1306:1323	inflammatory genes	1306:1323	Across both basal and activated inflammatory states, PCB exposure caused greater expression of a subset of inflammatory genes in the hypothalamus and lower expression of genes involved in dopamine, serotonin, and opioid systems compared to oil controls.
29879404	3	39	from	gap	442:444	arg1	knowledge					449:457	knowledge	449:457	knowledge	449:457	This is an important gap in knowledge, as molecules typically associated with neuroinflammation also serve neuromodulatory roles and interact with hormones in normal brain development.
29879404	0	40	theme	polychlorinated	42:56	arg1	exposure					67:74	gestational polychlorinated biphenyl exposure	30:74	gestational polychlorinated biphenyl exposure	30:74	Sex differences in effects of gestational polychlorinated biphenyl exposure on hypothalamic neuroimmune and neuromodulator systems in neonatal rats.
29879404	7	41	theme	subset	1296:1301	arg1	expression					1355:1364	lower expression	1349:1364	lower expression of genes involved in dopamine, serotonin, and opioid systems compared to oil controls	1349:1450	Across both basal and activated inflammatory states, PCB exposure caused greater expression of a subset of inflammatory genes in the hypothalamus and lower expression of genes involved in dopamine, serotonin, and opioid systems compared to oil controls.
29879404	7	41	theme	subset	1296:1301	arg1	expression					1280:1289	greater expression	1272:1289	greater expression of a subset of inflammatory genes in the hypothalamus	1272:1343	Across both basal and activated inflammatory states, PCB exposure caused greater expression of a subset of inflammatory genes in the hypothalamus and lower expression of genes involved in dopamine, serotonin, and opioid systems compared to oil controls.
29879404	8	42	theme	abnormal	1595:1602	arg1	increase					1604:1611	an abnormal increase	1592:1611	an abnormal increase in IL-1b and IL-6 serum concentration in response to LPS	1592:1668	PCB exposure also altered reactions to inflammatory challenge: it reversed the normal decrease in Esr2 hypothalamic expression and induced an abnormal increase in IL-1b and IL-6 serum concentration in response to LPS.
29879404	7	43	theme	greater	1272:1278	arg1	expression					1280:1289	greater expression	1272:1289	greater expression of a subset of inflammatory genes in the hypothalamus	1272:1343	Across both basal and activated inflammatory states, PCB exposure caused greater expression of a subset of inflammatory genes in the hypothalamus and lower expression of genes involved in dopamine, serotonin, and opioid systems compared to oil controls.
29879404	10	44	theme	potential	1722:1730	arg1	consequences					1742:1753	the potential long-term consequences	1718:1753	the potential long-term consequences of neuroimmune disruption	1718:1779	Given the potential long-term consequences of neuroimmune disruption, our findings demonstrate the need for further research.
29879404	1	45	theme	immune	256:261	arg1	dysfunction					285:295	immune, endocrine and neural dysfunction	256:295	immune, endocrine and neural dysfunction	256:295	Polychlorinated biphenyls (PCBs) are ubiquitous in the environment and exposure to them is associated with immune, endocrine and neural dysfunction.
29879404	8	46	theme	serum	1631:1635	arg1	concentration					1637:1649	IL-6 serum concentration	1626:1649	IL-6 serum concentration	1626:1649	PCB exposure also altered reactions to inflammatory challenge: it reversed the normal decrease in Esr2 hypothalamic expression and induced an abnormal increase in IL-1b and IL-6 serum concentration in response to LPS.
29879404	1	47	theme	endocrine	264:272	arg1	dysfunction					285:295	immune, endocrine and neural dysfunction	256:295	immune, endocrine and neural dysfunction	256:295	Polychlorinated biphenyls (PCBs) are ubiquitous in the environment and exposure to them is associated with immune, endocrine and neural dysfunction.
29879404	8	48	from	increase	1604:1611	arg1	concentration					1637:1649	IL-6 serum concentration	1626:1649	IL-6 serum concentration	1626:1649	PCB exposure also altered reactions to inflammatory challenge: it reversed the normal decrease in Esr2 hypothalamic expression and induced an abnormal increase in IL-1b and IL-6 serum concentration in response to LPS.
29879404	8	48	from	increase	1604:1611	arg1	IL-1b					1616:1620	IL-1b	1616:1620	IL-1b	1616:1620	PCB exposure also altered reactions to inflammatory challenge: it reversed the normal decrease in Esr2 hypothalamic expression and induced an abnormal increase in IL-1b and IL-6 serum concentration in response to LPS.
29879404	8	48	from	increase	1604:1611	arg1	response					1654:1661	response	1654:1661	response to LPS	1654:1668	PCB exposure also altered reactions to inflammatory challenge: it reversed the normal decrease in Esr2 hypothalamic expression and induced an abnormal increase in IL-1b and IL-6 serum concentration in response to LPS.
29879404	10	49	theme	neuroimmune	1758:1768	arg1	disruption					1770:1779	neuroimmune disruption	1758:1779	neuroimmune disruption	1758:1779	Given the potential long-term consequences of neuroimmune disruption, our findings demonstrate the need for further research.
29879404	5	50	theme	relevant	881:888	arg1	dose					890:893	an environmentally relevant dose	862:893	an environmentally relevant dose	862:893	Dams were fed wafers containing a mixture of PCBs at an environmentally relevant dose and composition (20 μg/kg, 1:1:1 Aroclor 1242:1248:1254) or oil vehicle control throughout their pregnancy.
29879404	7	51	from	expression	1280:1289	arg1	hypothalamus					1332:1343	the hypothalamus	1328:1343	the hypothalamus	1328:1343	Across both basal and activated inflammatory states, PCB exposure caused greater expression of a subset of inflammatory genes in the hypothalamus and lower expression of genes involved in dopamine, serotonin, and opioid systems compared to oil controls.
29879404	7	52	theme	inflammatory	1231:1242	arg1	states					1244:1249	both basal and activated inflammatory states	1206:1249	both basal and activated inflammatory states	1206:1249	Across both basal and activated inflammatory states, PCB exposure caused greater expression of a subset of inflammatory genes in the hypothalamus and lower expression of genes involved in dopamine, serotonin, and opioid systems compared to oil controls.
29879404	4	53	theme	PCB	679:681	arg1	exposure					683:690	gestational PCB exposure	667:690	gestational PCB exposure	667:690	The current study used Sprague-Dawley rats to assess whether gestational PCB exposure altered hypothalamic gene expression and serum cytokine concentration in neonatal animals given an immune challenge.
29879404	2	54	theme	PCBs	309:312	arg1	Effects					298:304	Effects	298:304	Effects of PCBs on inflammation and immunity	298:341	Effects of PCBs on inflammation and immunity are best described in spleen and blood, with fewer studies on neural tissues.
29879404	5	55	theme	PCBs	854:857	arg1	mixture					843:849	a mixture	841:849	a mixture of PCBs	841:857	Dams were fed wafers containing a mixture of PCBs at an environmentally relevant dose and composition (20 μg/kg, 1:1:1 Aroclor 1242:1248:1254) or oil vehicle control throughout their pregnancy.
29879404	0	56	from	neuroimmune	92:102	arg1	differences					4:14	Sex differences	0:14	Sex differences in effects of gestational polychlorinated biphenyl exposure on hypothalamic neuroimmune and neuromodulator systems in neonatal rats.	0:147	Sex differences in effects of gestational polychlorinated biphenyl exposure on hypothalamic neuroimmune and neuromodulator systems in neonatal rats.
29879404	0	56	from	neuroimmune	92:102	arg1	rats					143:146	neonatal rats	134:146	neonatal rats	134:146	Sex differences in effects of gestational polychlorinated biphenyl exposure on hypothalamic neuroimmune and neuromodulator systems in neonatal rats.
29879404	4	57	theme	Sprague-Dawley	629:642	arg1	rats					644:647	Sprague-Dawley rats	629:647	Sprague-Dawley rats	629:647	The current study used Sprague-Dawley rats to assess whether gestational PCB exposure altered hypothalamic gene expression and serum cytokine concentration in neonatal animals given an immune challenge.
29879404	6	58	theme	saline	1128:1133	arg1	control					1143:1149	an inflammatory challenge (lipopolysaccharide, LPS, 50 μg/kg, sc) or saline vehicle control	1059:1149	control	1143:1149	One day old male and female offspring were treated with an inflammatory challenge (lipopolysaccharide, LPS, 50 μg/kg, sc) or saline vehicle control approximately 3.5 h prior to tissue collection.
29879404	7	59	theme	serotonin	1397:1405	arg1	systems					1419:1425	dopamine, serotonin, and opioid systems	1387:1425	dopamine, serotonin, and opioid systems	1387:1425	Across both basal and activated inflammatory states, PCB exposure caused greater expression of a subset of inflammatory genes in the hypothalamus and lower expression of genes involved in dopamine, serotonin, and opioid systems compared to oil controls.
29879404	7	60	theme	basal	1211:1215	arg1	states					1244:1249	both basal and activated inflammatory states	1206:1249	both basal and activated inflammatory states	1206:1249	Across both basal and activated inflammatory states, PCB exposure caused greater expression of a subset of inflammatory genes in the hypothalamus and lower expression of genes involved in dopamine, serotonin, and opioid systems compared to oil controls.
29879404	3	61	theme	normal	580:585	arg1	development					593:603	normal brain development	580:603	normal brain development	580:603	This is an important gap in knowledge, as molecules typically associated with neuroinflammation also serve neuromodulatory roles and interact with hormones in normal brain development.
29879404	7	62	theme	dopamine	1387:1394	arg1	systems					1419:1425	dopamine, serotonin, and opioid systems	1387:1425	dopamine, serotonin, and opioid systems	1387:1425	Across both basal and activated inflammatory states, PCB exposure caused greater expression of a subset of inflammatory genes in the hypothalamus and lower expression of genes involved in dopamine, serotonin, and opioid systems compared to oil controls.
29879404	8	63	theme	inflammatory	1492:1503	arg1	challenge					1505:1513	inflammatory challenge	1492:1513	inflammatory challenge	1492:1513	PCB exposure also altered reactions to inflammatory challenge: it reversed the normal decrease in Esr2 hypothalamic expression and induced an abnormal increase in IL-1b and IL-6 serum concentration in response to LPS.
29879404	4	64	theme	cytokine	739:746	arg1	concentration					748:760	serum cytokine concentration	733:760	serum cytokine concentration	733:760	The current study used Sprague-Dawley rats to assess whether gestational PCB exposure altered hypothalamic gene expression and serum cytokine concentration in neonatal animals given an immune challenge.
29879404	3	65	from	hormones	568:575	arg1	development					593:603	normal brain development	580:603	normal brain development	580:603	This is an important gap in knowledge, as molecules typically associated with neuroinflammation also serve neuromodulatory roles and interact with hormones in normal brain development.
29879404	0	66	from	effects	19:25	arg1	neuroimmune					92:102	hypothalamic neuroimmune and neuromodulator systems	79:129	neuroimmune	92:102	Sex differences in effects of gestational polychlorinated biphenyl exposure on hypothalamic neuroimmune and neuromodulator systems in neonatal rats.
29879404	0	66	from	effects	19:25	arg1	systems					123:129	hypothalamic neuroimmune and neuromodulator systems	79:129	systems	123:129	Sex differences in effects of gestational polychlorinated biphenyl exposure on hypothalamic neuroimmune and neuromodulator systems in neonatal rats.
29879404	7	67	theme	activated	1221:1229	arg1	states					1244:1249	both basal and activated inflammatory states	1206:1249	both basal and activated inflammatory states	1206:1249	Across both basal and activated inflammatory states, PCB exposure caused greater expression of a subset of inflammatory genes in the hypothalamus and lower expression of genes involved in dopamine, serotonin, and opioid systems compared to oil controls.
29879404	1	68	from	ubiquitous	186:195	arg1	environment					204:214	the environment	200:214	the environment	200:214	Polychlorinated biphenyls (PCBs) are ubiquitous in the environment and exposure to them is associated with immune, endocrine and neural dysfunction.
29879404	0	69	theme	neonatal	134:141	arg1	rats					143:146	neonatal rats	134:146	neonatal rats	134:146	Sex differences in effects of gestational polychlorinated biphenyl exposure on hypothalamic neuroimmune and neuromodulator systems in neonatal rats.
29879404	0	70	from	differences	4:14	arg1	effects					19:25	effects	19:25	effects of gestational polychlorinated biphenyl exposure on hypothalamic neuroimmune and neuromodulator systems in neonatal rats	19:146	Sex differences in effects of gestational polychlorinated biphenyl exposure on hypothalamic neuroimmune and neuromodulator systems in neonatal rats.
29879404	0	70	from	differences	4:14	arg1	systems					123:129	hypothalamic neuroimmune and neuromodulator systems	79:129	systems	123:129	Sex differences in effects of gestational polychlorinated biphenyl exposure on hypothalamic neuroimmune and neuromodulator systems in neonatal rats.
29879404	0	70	from	differences	4:14	arg1	neuroimmune					92:102	hypothalamic neuroimmune and neuromodulator systems	79:129	neuroimmune	92:102	Sex differences in effects of gestational polychlorinated biphenyl exposure on hypothalamic neuroimmune and neuromodulator systems in neonatal rats.
29879404	4	71	theme	gene	713:716	arg1	expression					718:727	hypothalamic gene expression	700:727	hypothalamic gene expression	700:727	The current study used Sprague-Dawley rats to assess whether gestational PCB exposure altered hypothalamic gene expression and serum cytokine concentration in neonatal animals given an immune challenge.
29879404	8	72	theme	PCB	1453:1455	arg1	exposure					1457:1464	PCB exposure	1453:1464	PCB exposure	1453:1464	PCB exposure also altered reactions to inflammatory challenge: it reversed the normal decrease in Esr2 hypothalamic expression and induced an abnormal increase in IL-1b and IL-6 serum concentration in response to LPS.
29879404	0	73	theme	neuromodulator	108:121	arg1	systems					123:129	hypothalamic neuroimmune and neuromodulator systems	79:129	systems	123:129	Sex differences in effects of gestational polychlorinated biphenyl exposure on hypothalamic neuroimmune and neuromodulator systems in neonatal rats.
29879404	8	74	theme	normal	1532:1537	arg1	decrease					1539:1546	the normal decrease	1528:1546	the normal decrease in Esr2 hypothalamic expression	1528:1578	PCB exposure also altered reactions to inflammatory challenge: it reversed the normal decrease in Esr2 hypothalamic expression and induced an abnormal increase in IL-1b and IL-6 serum concentration in response to LPS.
29879404	5	75	theme	oil	955:957	arg1	vehicle					959:965	oil vehicle	955:965	oil vehicle	955:965	Dams were fed wafers containing a mixture of PCBs at an environmentally relevant dose and composition (20 μg/kg, 1:1:1 Aroclor 1242:1248:1254) or oil vehicle control throughout their pregnancy.
29879404	8	76	theme	hypothalamic	1556:1567	arg1	expression					1569:1578	Esr2 hypothalamic expression	1551:1578	Esr2 hypothalamic expression	1551:1578	PCB exposure also altered reactions to inflammatory challenge: it reversed the normal decrease in Esr2 hypothalamic expression and induced an abnormal increase in IL-1b and IL-6 serum concentration in response to LPS.
29879404	7	77	theme	genes	1319:1323	arg1	subset					1296:1301	a subset	1294:1301	a subset of inflammatory genes	1294:1323	Across both basal and activated inflammatory states, PCB exposure caused greater expression of a subset of inflammatory genes in the hypothalamus and lower expression of genes involved in dopamine, serotonin, and opioid systems compared to oil controls.
29879404	2	78	theme	neural	405:410	arg1	tissues					412:418	neural tissues	405:418	neural tissues	405:418	Effects of PCBs on inflammation and immunity are best described in spleen and blood, with fewer studies on neural tissues.
29879404	0	79	theme	gestational	30:40	arg1	exposure					67:74	gestational polychlorinated biphenyl exposure	30:74	gestational polychlorinated biphenyl exposure	30:74	Sex differences in effects of gestational polychlorinated biphenyl exposure on hypothalamic neuroimmune and neuromodulator systems in neonatal rats.
29879404	0	80	theme	biphenyl	58:65	arg1	exposure					67:74	gestational polychlorinated biphenyl exposure	30:74	gestational polychlorinated biphenyl exposure	30:74	Sex differences in effects of gestational polychlorinated biphenyl exposure on hypothalamic neuroimmune and neuromodulator systems in neonatal rats.
29879404	6	81	theme	vehicle	1135:1141	arg1	control					1143:1149	an inflammatory challenge (lipopolysaccharide, LPS, 50 μg/kg, sc) or saline vehicle control	1059:1149	control	1143:1149	One day old male and female offspring were treated with an inflammatory challenge (lipopolysaccharide, LPS, 50 μg/kg, sc) or saline vehicle control approximately 3.5 h prior to tissue collection.
29879404	5	82	dep	dose	890:893	arg1	control					967:973	control	967:973	control	967:973	Dams were fed wafers containing a mixture of PCBs at an environmentally relevant dose and composition (20 μg/kg, 1:1:1 Aroclor 1242:1248:1254) or oil vehicle control throughout their pregnancy.
29879404	5	82	dep	dose	890:893	arg1	20 μg/kg					912:919	20 μg/kg	912:919	20 μg/kg	912:919	Dams were fed wafers containing a mixture of PCBs at an environmentally relevant dose and composition (20 μg/kg, 1:1:1 Aroclor 1242:1248:1254) or oil vehicle control throughout their pregnancy.
29879404	5	82	dep	dose	890:893	arg1	Aroclor					928:934	1:1:1 Aroclor 1242:1248:1254	922:949	1:1:1 Aroclor 1242:1248:1254	922:949	Dams were fed wafers containing a mixture of PCBs at an environmentally relevant dose and composition (20 μg/kg, 1:1:1 Aroclor 1242:1248:1254) or oil vehicle control throughout their pregnancy.
29879404	4	83	from	concentration	748:760	arg1	animals					774:780	neonatal animals	765:780	neonatal animals given an immune challenge	765:806	The current study used Sprague-Dawley rats to assess whether gestational PCB exposure altered hypothalamic gene expression and serum cytokine concentration in neonatal animals given an immune challenge.
29879404	4	84	theme	current	610:616	arg1	study					618:622	The current study	606:622	The current study	606:622	The current study used Sprague-Dawley rats to assess whether gestational PCB exposure altered hypothalamic gene expression and serum cytokine concentration in neonatal animals given an immune challenge.
29879404	2	85	from	studies	394:400	arg1	tissues					412:418	neural tissues	405:418	neural tissues	405:418	Effects of PCBs on inflammation and immunity are best described in spleen and blood, with fewer studies on neural tissues.
29879404	8	86	theme	IL-6	1626:1629	arg1	concentration					1637:1649	IL-6 serum concentration	1626:1649	IL-6 serum concentration	1626:1649	PCB exposure also altered reactions to inflammatory challenge: it reversed the normal decrease in Esr2 hypothalamic expression and induced an abnormal increase in IL-1b and IL-6 serum concentration in response to LPS.
29879404	10	87	theme	long-term	1732:1740	arg1	consequences					1742:1753	the potential long-term consequences	1718:1753	the potential long-term consequences of neuroimmune disruption	1718:1779	Given the potential long-term consequences of neuroimmune disruption, our findings demonstrate the need for further research.
29879404	4	88	from	expression	718:727	arg1	animals					774:780	neonatal animals	765:780	neonatal animals given an immune challenge	765:806	The current study used Sprague-Dawley rats to assess whether gestational PCB exposure altered hypothalamic gene expression and serum cytokine concentration in neonatal animals given an immune challenge.
30747616	3	0	theme	gene	285:288	arg1	sequences					290:298	16S rRNA gene sequences	276:298	16S rRNA gene sequences	276:298	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 3-2-2T was a member of the genus Bacillus and most closely related to Bacillus fortis R-6514T (98.9 % similarity), Bacillus terrae RA9T (98.0 %) and Bacillus fordii R-7190T (97.7 %).
30747616	2	1	dep	Gram-stain-positive	64:82	arg1	motile					97:102	motile	97:102	motile	97:102	A Gram-stain-positive, rod-shaped, motile bacterial strain, designated 3-2-2T, was isolated from field topsoil collected from a western suburb of Nanyang city, Henan province, China.
30747616	2	1	dep	Gram-stain-positive	64:82	arg1	rod-shaped					85:94	rod-shaped	85:94	rod-shaped	85:94	A Gram-stain-positive, rod-shaped, motile bacterial strain, designated 3-2-2T, was isolated from field topsoil collected from a western suburb of Nanyang city, Henan province, China.
30747616	3	2	dep	member	335:340	arg1	%					501:501	97.7 %	496:501	97.7 %	496:501	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 3-2-2T was a member of the genus Bacillus and most closely related to Bacillus fortis R-6514T (98.9 % similarity), Bacillus terrae RA9T (98.0 %) and Bacillus fordii R-7190T (97.7 %).
30747616	9	3	theme	unidentified	1078:1089	arg1	phospholipid					1091:1102	an unidentified phospholipid	1075:1102	an unidentified phospholipid	1075:1102	The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unidentified phospholipid and an unidentified lipid.
30747616	10	4	theme	genomic	1179:1185	arg1	comparisons					1187:1197	in silico genomic comparisons	1169:1197	in silico genomic comparisons	1169:1197	The results of phylogenetic analyses, in silico genomic comparisons, and chemotaxonomic and phenotypic analyses clearly indicated that strain 3-2-2T represents a novel species within the genus Bacillus, for which the name Bacillus acidinfaciens sp.
30747616	2	5	theme	bacterial	104:112	arg1	strain					114:119	A Gram-stain-positive, rod-shaped, motile bacterial strain	62:119	A Gram-stain-positive, rod-shaped, motile bacterial strain	62:119	A Gram-stain-positive, rod-shaped, motile bacterial strain, designated 3-2-2T, was isolated from field topsoil collected from a western suburb of Nanyang city, Henan province, China.
30747616	8	6	theme	meso-Diaminopimelic	906:924	arg1	acid					926:929	meso-Diaminopimelic acid	906:929	meso-Diaminopimelic acid	906:929	meso-Diaminopimelic acid was detected in the peptidoglycan.
30747616	5	7	theme	digital	635:641	arg1	hybridization					651:663	digital DNA-DNA hybridization	635:663	digital DNA-DNA hybridization	635:663	The average nucleotide identity and digital DNA-DNA hybridization values between 3-2-2T and the closely related Bacillus species ranged 79.4-84.2 % and 23.4-24.6 %.
30747616	10	8	theme	acidinfaciens	1362:1374	arg1	sp					1376:1377	Bacillus acidinfaciens sp	1353:1377	the name Bacillus acidinfaciens sp	1344:1377	The results of phylogenetic analyses, in silico genomic comparisons, and chemotaxonomic and phenotypic analyses clearly indicated that strain 3-2-2T represents a novel species within the genus Bacillus, for which the name Bacillus acidinfaciens sp.
30747616	10	9	dep	in	1169:1170	arg1	silico					1172:1177	silico	1172:1177	silico	1172:1177	The results of phylogenetic analyses, in silico genomic comparisons, and chemotaxonomic and phenotypic analyses clearly indicated that strain 3-2-2T represents a novel species within the genus Bacillus, for which the name Bacillus acidinfaciens sp.
30747616	1	10	theme	farmland	47:54	arg1	soil					56:59	farmland soil	47:59	farmland soil	47:59	nov., isolated from farmland soil.
30747616	10	11	theme	in	1169:1170	arg1	comparisons					1187:1197	in silico genomic comparisons	1169:1197	in silico genomic comparisons	1169:1197	The results of phylogenetic analyses, in silico genomic comparisons, and chemotaxonomic and phenotypic analyses clearly indicated that strain 3-2-2T represents a novel species within the genus Bacillus, for which the name Bacillus acidinfaciens sp.
30747616	5	12	theme	DNA-DNA	643:649	arg1	hybridization					651:663	digital DNA-DNA hybridization	635:663	digital DNA-DNA hybridization	635:663	The average nucleotide identity and digital DNA-DNA hybridization values between 3-2-2T and the closely related Bacillus species ranged 79.4-84.2 % and 23.4-24.6 %.
30747616	12	13	theme	=CGMCC	1425:1430	arg1	30839T					1445:1450	=CGMCC 1.13685T=LMG 30839T	1425:1450	=CGMCC 1.13685T=LMG 30839T	1425:1450	The type strain is 3-2-2T (=CGMCC 1.13685T=LMG 30839T).
30747616	12	13	theme	=CGMCC	1425:1430	arg1	3-2-2T					1417:1422	3-2-2T	1417:1422	3-2-2T (=CGMCC 1.13685T=LMG 30839T)	1417:1451	The type strain is 3-2-2T (=CGMCC 1.13685T=LMG 30839T).
30747616	3	14	dep	genus	349:353	arg1	the					345:347	the	345:347	the	345:347	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 3-2-2T was a member of the genus Bacillus and most closely related to Bacillus fortis R-6514T (98.9 % similarity), Bacillus terrae RA9T (98.0 %) and Bacillus fordii R-7190T (97.7 %).
30747616	3	14	dep	genus	349:353	arg1	Bacillus					355:362	Bacillus	355:362	Bacillus	355:362	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 3-2-2T was a member of the genus Bacillus and most closely related to Bacillus fortis R-6514T (98.9 % similarity), Bacillus terrae RA9T (98.0 %) and Bacillus fordii R-7190T (97.7 %).
30747616	0	15	theme	Bacillus	0:7	arg1	sp					23:24	Bacillus acidinfaciens sp	0:24	Bacillus acidinfaciens sp.	0:25	Bacillus acidinfaciens sp.
30747616	3	16	theme	strain	315:320	arg1	3-2-2T					322:327	strain 3-2-2T	315:327	strain 3-2-2T	315:327	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 3-2-2T was a member of the genus Bacillus and most closely related to Bacillus fortis R-6514T (98.9 % similarity), Bacillus terrae RA9T (98.0 %) and Bacillus fordii R-7190T (97.7 %).
30747616	3	16	theme	strain	315:320	arg1	member					335:340	a member	333:340	a member of the genus Bacillus and most closely related to Bacillus fortis R-6514T (98.9 % similarity), Bacillus terrae RA9T (98.0 %) and Bacillus fordii R-7190T	333:493	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 3-2-2T was a member of the genus Bacillus and most closely related to Bacillus fortis R-6514T (98.9 % similarity), Bacillus terrae RA9T (98.0 %) and Bacillus fordii R-7190T (97.7 %).
30747616	8	17	located	detected	935:942	arg1	peptidoglycan					951:963	the peptidoglycan	947:963	the peptidoglycan	947:963	meso-Diaminopimelic acid was detected in the peptidoglycan.
30747616	8	17	located	detected	935:942	arg2	acid					926:929	meso-Diaminopimelic acid	906:929	meso-Diaminopimelic acid	906:929	meso-Diaminopimelic acid was detected in the peptidoglycan.
30747616	10	18	theme	chemotaxonomic	1204:1217	arg1	analyses					1234:1241	chemotaxonomic and phenotypic analyses	1204:1241	chemotaxonomic and phenotypic analyses	1204:1241	The results of phylogenetic analyses, in silico genomic comparisons, and chemotaxonomic and phenotypic analyses clearly indicated that strain 3-2-2T represents a novel species within the genus Bacillus, for which the name Bacillus acidinfaciens sp.
30747616	3	19	theme	Bacillus	392:399	arg1	RA9T					453:456	Bacillus terrae RA9T	437:456	Bacillus terrae RA9T (98.0 %)	437:465	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 3-2-2T was a member of the genus Bacillus and most closely related to Bacillus fortis R-6514T (98.9 % similarity), Bacillus terrae RA9T (98.0 %) and Bacillus fordii R-7190T (97.7 %).
30747616	3	19	theme	Bacillus	392:399	arg1	similarity					424:433	98.9 % similarity	417:433	98.9 % similarity	417:433	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 3-2-2T was a member of the genus Bacillus and most closely related to Bacillus fortis R-6514T (98.9 % similarity), Bacillus terrae RA9T (98.0 %) and Bacillus fordii R-7190T (97.7 %).
30747616	3	19	theme	Bacillus	392:399	arg1	R-6514T					408:414	Bacillus fortis R-6514T	392:414	Bacillus fortis R-6514T (98.9 % similarity)	392:434	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 3-2-2T was a member of the genus Bacillus and most closely related to Bacillus fortis R-6514T (98.9 % similarity), Bacillus terrae RA9T (98.0 %) and Bacillus fordii R-7190T (97.7 %).
30747616	6	20	theme	3-2-2T	796:801	arg1	iso-C15 					808:815	iso-C15 	808:815	iso-C15 	808:815	The major fatty acids of strain 3-2-2T were iso-C15 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C16 : 0.
30747616	6	20	theme	3-2-2T	796:801	arg1	acids					780:784	The major fatty acids	764:784	The major fatty acids of strain 3-2-2T	764:801	The major fatty acids of strain 3-2-2T were iso-C15 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C16 : 0.
30747616	10	21	theme	strain	1266:1271	arg1	3-2-2T					1273:1278	strain 3-2-2T	1266:1278	strain 3-2-2T	1266:1278	The results of phylogenetic analyses, in silico genomic comparisons, and chemotaxonomic and phenotypic analyses clearly indicated that strain 3-2-2T represents a novel species within the genus Bacillus, for which the name Bacillus acidinfaciens sp.
30747616	2	22	theme	China	238:242	arg1	suburb					198:203	a western suburb	188:203	a western suburb of Nanyang city, Henan province, China	188:242	A Gram-stain-positive, rod-shaped, motile bacterial strain, designated 3-2-2T, was isolated from field topsoil collected from a western suburb of Nanyang city, Henan province, China.
30747616	5	23	theme	hybridization	651:663	arg1	values					665:670	The average nucleotide identity and digital DNA-DNA hybridization values	599:670	The average nucleotide identity and digital DNA-DNA hybridization values between 3-2-2T and the closely related Bacillus species	599:726	The average nucleotide identity and digital DNA-DNA hybridization values between 3-2-2T and the closely related Bacillus species ranged 79.4-84.2 % and 23.4-24.6 %.
30747616	0	24	theme	acidinfaciens	9:21	arg1	sp					23:24	Bacillus acidinfaciens sp	0:24	Bacillus acidinfaciens sp.	0:25	Bacillus acidinfaciens sp.
30747616	6	25	theme	strain	789:794	arg1	3-2-2T					796:801	strain 3-2-2T	789:801	strain 3-2-2T	789:801	The major fatty acids of strain 3-2-2T were iso-C15 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C16 : 0.
30747616	10	26	theme	Bacillus	1353:1360	arg1	sp					1376:1377	Bacillus acidinfaciens sp	1353:1377	the name Bacillus acidinfaciens sp	1344:1377	The results of phylogenetic analyses, in silico genomic comparisons, and chemotaxonomic and phenotypic analyses clearly indicated that strain 3-2-2T represents a novel species within the genus Bacillus, for which the name Bacillus acidinfaciens sp.
30747616	9	27	theme	polar	970:974	arg1	profile					982:988	The polar lipid profile	966:988	The polar lipid profile	966:988	The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unidentified phospholipid and an unidentified lipid.
30747616	3	28	theme	related	381:387	arg1	R-7190T					487:493	the genus Bacillus and most closely related to Bacillus fortis R-6514T (98.9 % similarity), Bacillus terrae RA9T (98.0 %) and Bacillus fordii R-7190T	345:493	the genus Bacillus and most closely related to Bacillus fortis R-6514T (98.9 % similarity), Bacillus terrae RA9T (98.0 %) and Bacillus fordii R-7190T	345:493	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 3-2-2T was a member of the genus Bacillus and most closely related to Bacillus fortis R-6514T (98.9 % similarity), Bacillus terrae RA9T (98.0 %) and Bacillus fordii R-7190T (97.7 %).
30747616	3	29	theme	genus	349:353	arg1	R-7190T					487:493	the genus Bacillus and most closely related to Bacillus fortis R-6514T (98.9 % similarity), Bacillus terrae RA9T (98.0 %) and Bacillus fordii R-7190T	345:493	the genus Bacillus and most closely related to Bacillus fortis R-6514T (98.9 % similarity), Bacillus terrae RA9T (98.0 %) and Bacillus fordii R-7190T	345:493	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 3-2-2T was a member of the genus Bacillus and most closely related to Bacillus fortis R-6514T (98.9 % similarity), Bacillus terrae RA9T (98.0 %) and Bacillus fordii R-7190T (97.7 %).
30747616	12	30	theme	type	1402:1405	arg1	strain					1407:1412	The type strain	1398:1412	The type strain	1398:1412	The type strain is 3-2-2T (=CGMCC 1.13685T=LMG 30839T).
30747616	12	30	theme	type	1402:1405	arg1	3-2-2T					1417:1422	3-2-2T	1417:1422	3-2-2T (=CGMCC 1.13685T=LMG 30839T)	1417:1451	The type strain is 3-2-2T (=CGMCC 1.13685T=LMG 30839T).
30747616	9	31	theme	lipid	976:980	arg1	profile					982:988	The polar lipid profile	966:988	The polar lipid profile	966:988	The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unidentified phospholipid and an unidentified lipid.
30747616	5	32	theme	%	745:745	arg1	%					761:761	79.4-84.2 % and 23.4-24.6 %	735:761	79.4-84.2 % and 23.4-24.6 %	735:761	The average nucleotide identity and digital DNA-DNA hybridization values between 3-2-2T and the closely related Bacillus species ranged 79.4-84.2 % and 23.4-24.6 %.
30747616	5	33	theme	79.4-84.2 	735:744	arg1	%					761:761	79.4-84.2 % and 23.4-24.6 %	735:761	79.4-84.2 % and 23.4-24.6 %	735:761	The average nucleotide identity and digital DNA-DNA hybridization values between 3-2-2T and the closely related Bacillus species ranged 79.4-84.2 % and 23.4-24.6 %.
30747616	3	34	theme	Phylogenetic	245:256	arg1	analysis					258:265	Phylogenetic analysis	245:265	Phylogenetic analysis based on 16S rRNA gene sequences	245:298	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 3-2-2T was a member of the genus Bacillus and most closely related to Bacillus fortis R-6514T (98.9 % similarity), Bacillus terrae RA9T (98.0 %) and Bacillus fordii R-7190T (97.7 %).
30747616	9	35	theme	unidentified	1111:1122	arg1	lipid					1124:1128	an unidentified lipid	1108:1128	an unidentified lipid	1108:1128	The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unidentified phospholipid and an unidentified lipid.
30747616	3	36	theme	fordii	480:485	arg1	R-7190T					487:493	the genus Bacillus and most closely related to Bacillus fortis R-6514T (98.9 % similarity), Bacillus terrae RA9T (98.0 %) and Bacillus fordii R-7190T	345:493	the genus Bacillus and most closely related to Bacillus fortis R-6514T (98.9 % similarity), Bacillus terrae RA9T (98.0 %) and Bacillus fordii R-7190T	345:493	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 3-2-2T was a member of the genus Bacillus and most closely related to Bacillus fortis R-6514T (98.9 % similarity), Bacillus terrae RA9T (98.0 %) and Bacillus fordii R-7190T (97.7 %).
30747616	3	37	theme	98.9 	417:421	arg1	similarity					424:433	98.9 % similarity	417:433	98.9 % similarity	417:433	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 3-2-2T was a member of the genus Bacillus and most closely related to Bacillus fortis R-6514T (98.9 % similarity), Bacillus terrae RA9T (98.0 %) and Bacillus fordii R-7190T (97.7 %).
30747616	3	37	theme	98.9 	417:421	arg1	R-6514T					408:414	Bacillus fortis R-6514T	392:414	Bacillus fortis R-6514T (98.9 % similarity)	392:434	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 3-2-2T was a member of the genus Bacillus and most closely related to Bacillus fortis R-6514T (98.9 % similarity), Bacillus terrae RA9T (98.0 %) and Bacillus fordii R-7190T (97.7 %).
30747616	5	38	theme	 and	746:749	arg1	%					761:761	79.4-84.2 % and 23.4-24.6 %	735:761	79.4-84.2 % and 23.4-24.6 %	735:761	The average nucleotide identity and digital DNA-DNA hybridization values between 3-2-2T and the closely related Bacillus species ranged 79.4-84.2 % and 23.4-24.6 %.
30747616	5	39	theme	nucleotide	611:620	arg1	identity					622:629	average nucleotide identity	603:629	average nucleotide identity	603:629	The average nucleotide identity and digital DNA-DNA hybridization values between 3-2-2T and the closely related Bacillus species ranged 79.4-84.2 % and 23.4-24.6 %.
30747616	3	40	theme	R-7190T	487:493	arg1	3-2-2T					322:327	strain 3-2-2T	315:327	strain 3-2-2T	315:327	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 3-2-2T was a member of the genus Bacillus and most closely related to Bacillus fortis R-6514T (98.9 % similarity), Bacillus terrae RA9T (98.0 %) and Bacillus fordii R-7190T (97.7 %).
30747616	3	40	theme	R-7190T	487:493	arg1	member					335:340	a member	333:340	a member of the genus Bacillus and most closely related to Bacillus fortis R-6514T (98.9 % similarity), Bacillus terrae RA9T (98.0 %) and Bacillus fordii R-7190T	333:493	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 3-2-2T was a member of the genus Bacillus and most closely related to Bacillus fortis R-6514T (98.9 % similarity), Bacillus terrae RA9T (98.0 %) and Bacillus fordii R-7190T (97.7 %).
30747616	10	41	theme	analyses	1234:1241	arg1	results					1135:1141	The results	1131:1141	The results of phylogenetic analyses, in silico genomic comparisons, and chemotaxonomic and phenotypic analyses	1131:1241	The results of phylogenetic analyses, in silico genomic comparisons, and chemotaxonomic and phenotypic analyses clearly indicated that strain 3-2-2T represents a novel species within the genus Bacillus, for which the name Bacillus acidinfaciens sp.
30747616	3	42	theme	%	422:422	arg1	similarity					424:433	98.9 % similarity	417:433	98.9 % similarity	417:433	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 3-2-2T was a member of the genus Bacillus and most closely related to Bacillus fortis R-6514T (98.9 % similarity), Bacillus terrae RA9T (98.0 %) and Bacillus fordii R-7190T (97.7 %).
30747616	3	42	theme	%	422:422	arg1	R-6514T					408:414	Bacillus fortis R-6514T	392:414	Bacillus fortis R-6514T (98.9 % similarity)	392:434	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 3-2-2T was a member of the genus Bacillus and most closely related to Bacillus fortis R-6514T (98.9 % similarity), Bacillus terrae RA9T (98.0 %) and Bacillus fordii R-7190T (97.7 %).
30747616	5	43	theme	identity	622:629	arg1	values					665:670	The average nucleotide identity and digital DNA-DNA hybridization values	599:670	The average nucleotide identity and digital DNA-DNA hybridization values between 3-2-2T and the closely related Bacillus species	599:726	The average nucleotide identity and digital DNA-DNA hybridization values between 3-2-2T and the closely related Bacillus species ranged 79.4-84.2 % and 23.4-24.6 %.
30747616	2	44	theme	field	159:163	arg1	topsoil					165:171	field topsoil	159:171	field topsoil collected from a western suburb of Nanyang city, Henan province, China	159:242	A Gram-stain-positive, rod-shaped, motile bacterial strain, designated 3-2-2T, was isolated from field topsoil collected from a western suburb of Nanyang city, Henan province, China.
30747616	10	45	theme	phenotypic	1223:1232	arg1	analyses					1234:1241	chemotaxonomic and phenotypic analyses	1204:1241	chemotaxonomic and phenotypic analyses	1204:1241	The results of phylogenetic analyses, in silico genomic comparisons, and chemotaxonomic and phenotypic analyses clearly indicated that strain 3-2-2T represents a novel species within the genus Bacillus, for which the name Bacillus acidinfaciens sp.
30747616	2	46	attach	isolated	145:152	arg1	topsoil					165:171	field topsoil	159:171	field topsoil collected from a western suburb of Nanyang city, Henan province, China	159:242	A Gram-stain-positive, rod-shaped, motile bacterial strain, designated 3-2-2T, was isolated from field topsoil collected from a western suburb of Nanyang city, Henan province, China.
30747616	2	46	attach	isolated	145:152	arg2	strain					114:119	A Gram-stain-positive, rod-shaped, motile bacterial strain	62:119	A Gram-stain-positive, rod-shaped, motile bacterial strain	62:119	A Gram-stain-positive, rod-shaped, motile bacterial strain, designated 3-2-2T, was isolated from field topsoil collected from a western suburb of Nanyang city, Henan province, China.
30747616	4	47	theme	DNA	569:571	arg1	G+C content					573:583	a DNA G+C content	567:583	a DNA G+C content of 42.2 mol%	567:596	A draft genome sequence determined for strain 3-2-2T revealed a DNA G+C content of 42.2 mol%.
30747616	5	48	theme	related	703:709	arg1	species					720:726	the closely related Bacillus species	691:726	the closely related Bacillus species	691:726	The average nucleotide identity and digital DNA-DNA hybridization values between 3-2-2T and the closely related Bacillus species ranged 79.4-84.2 % and 23.4-24.6 %.
30747616	6	49	theme	fatty	774:778	arg1	iso-C15 					808:815	iso-C15 	808:815	iso-C15 	808:815	The major fatty acids of strain 3-2-2T were iso-C15 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C16 : 0.
30747616	6	49	theme	fatty	774:778	arg1	acids					780:784	The major fatty acids	764:784	The major fatty acids of strain 3-2-2T	764:801	The major fatty acids of strain 3-2-2T were iso-C15 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C16 : 0.
30747616	5	50	theme	Bacillus	711:718	arg1	species					720:726	the closely related Bacillus species	691:726	the closely related Bacillus species	691:726	The average nucleotide identity and digital DNA-DNA hybridization values between 3-2-2T and the closely related Bacillus species ranged 79.4-84.2 % and 23.4-24.6 %.
30747616	10	51	dep	genus	1318:1322	arg1	Bacillus					1324:1331	the genus Bacillus	1314:1331	the genus Bacillus	1314:1331	The results of phylogenetic analyses, in silico genomic comparisons, and chemotaxonomic and phenotypic analyses clearly indicated that strain 3-2-2T represents a novel species within the genus Bacillus, for which the name Bacillus acidinfaciens sp.
30747616	3	52	theme	16S	276:278	arg1	sequences					290:298	16S rRNA gene sequences	276:298	16S rRNA gene sequences	276:298	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 3-2-2T was a member of the genus Bacillus and most closely related to Bacillus fortis R-6514T (98.9 % similarity), Bacillus terrae RA9T (98.0 %) and Bacillus fordii R-7190T (97.7 %).
30747616	6	53	theme	major	768:772	arg1	iso-C15 					808:815	iso-C15 	808:815	iso-C15 	808:815	The major fatty acids of strain 3-2-2T were iso-C15 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C16 : 0.
30747616	6	53	theme	major	768:772	arg1	acids					780:784	The major fatty acids	764:784	The major fatty acids of strain 3-2-2T	764:801	The major fatty acids of strain 3-2-2T were iso-C15 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C16 : 0.
30747616	3	54	dep	Bacillus	392:399	arg1	fortis					401:406	fortis	401:406	fortis	401:406	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 3-2-2T was a member of the genus Bacillus and most closely related to Bacillus fortis R-6514T (98.9 % similarity), Bacillus terrae RA9T (98.0 %) and Bacillus fordii R-7190T (97.7 %).
30747616	12	55	theme	1.13685T=LMG	1432:1443	arg1	30839T					1445:1450	=CGMCC 1.13685T=LMG 30839T	1425:1450	=CGMCC 1.13685T=LMG 30839T	1425:1450	The type strain is 3-2-2T (=CGMCC 1.13685T=LMG 30839T).
30747616	12	55	theme	1.13685T=LMG	1432:1443	arg1	3-2-2T					1417:1422	3-2-2T	1417:1422	3-2-2T (=CGMCC 1.13685T=LMG 30839T)	1417:1451	The type strain is 3-2-2T (=CGMCC 1.13685T=LMG 30839T).
30747616	3	56	theme	rRNA	280:283	arg1	sequences					290:298	16S rRNA gene sequences	276:298	16S rRNA gene sequences	276:298	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 3-2-2T was a member of the genus Bacillus and most closely related to Bacillus fortis R-6514T (98.9 % similarity), Bacillus terrae RA9T (98.0 %) and Bacillus fordii R-7190T (97.7 %).
30747616	2	57	theme	western	190:196	arg1	suburb					198:203	a western suburb	188:203	a western suburb of Nanyang city, Henan province, China	188:242	A Gram-stain-positive, rod-shaped, motile bacterial strain, designated 3-2-2T, was isolated from field topsoil collected from a western suburb of Nanyang city, Henan province, China.
30747616	7	58	theme	major	871:875	arg1	MK-7					900:903	MK-7	900:903	MK-7	900:903	The major isoprenoid quinone was MK-7.
30747616	7	58	theme	major	871:875	arg1	quinone					888:894	The major isoprenoid quinone	867:894	The major isoprenoid quinone	867:894	The major isoprenoid quinone was MK-7.
30747616	3	59	theme	Bacillus	437:444	arg1	RA9T					453:456	Bacillus terrae RA9T	437:456	Bacillus terrae RA9T (98.0 %)	437:465	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 3-2-2T was a member of the genus Bacillus and most closely related to Bacillus fortis R-6514T (98.9 % similarity), Bacillus terrae RA9T (98.0 %) and Bacillus fordii R-7190T (97.7 %).
30747616	3	59	theme	Bacillus	437:444	arg1	%					464:464	98.0 %	459:464	98.0 %	459:464	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 3-2-2T was a member of the genus Bacillus and most closely related to Bacillus fortis R-6514T (98.9 % similarity), Bacillus terrae RA9T (98.0 %) and Bacillus fordii R-7190T (97.7 %).
30747616	3	59	theme	Bacillus	437:444	arg1	R-6514T					408:414	Bacillus fortis R-6514T	392:414	Bacillus fortis R-6514T (98.9 % similarity)	392:434	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 3-2-2T was a member of the genus Bacillus and most closely related to Bacillus fortis R-6514T (98.9 % similarity), Bacillus terrae RA9T (98.0 %) and Bacillus fordii R-7190T (97.7 %).
30747616	2	60	theme	Gram-stain-positive	64:82	arg1	strain					114:119	A Gram-stain-positive, rod-shaped, motile bacterial strain	62:119	A Gram-stain-positive, rod-shaped, motile bacterial strain	62:119	A Gram-stain-positive, rod-shaped, motile bacterial strain, designated 3-2-2T, was isolated from field topsoil collected from a western suburb of Nanyang city, Henan province, China.
30747616	7	61	theme	isoprenoid	877:886	arg1	MK-7					900:903	MK-7	900:903	MK-7	900:903	The major isoprenoid quinone was MK-7.
30747616	7	61	theme	isoprenoid	877:886	arg1	quinone					888:894	The major isoprenoid quinone	867:894	The major isoprenoid quinone	867:894	The major isoprenoid quinone was MK-7.
30747616	10	62	theme	analyses	1159:1166	arg1	results					1135:1141	The results	1131:1141	The results of phylogenetic analyses, in silico genomic comparisons, and chemotaxonomic and phenotypic analyses	1131:1241	The results of phylogenetic analyses, in silico genomic comparisons, and chemotaxonomic and phenotypic analyses clearly indicated that strain 3-2-2T represents a novel species within the genus Bacillus, for which the name Bacillus acidinfaciens sp.
30747616	4	63	theme	genome	513:518	arg1	sequence					520:527	A draft genome sequence	505:527	A draft genome sequence determined for strain 3-2-2T	505:556	A draft genome sequence determined for strain 3-2-2T revealed a DNA G+C content of 42.2 mol%.
30747616	3	64	dep	Bacillus	437:444	arg1	terrae					446:451	terrae	446:451	terrae	446:451	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 3-2-2T was a member of the genus Bacillus and most closely related to Bacillus fortis R-6514T (98.9 % similarity), Bacillus terrae RA9T (98.0 %) and Bacillus fordii R-7190T (97.7 %).
30747616	10	65	theme	phylogenetic	1146:1157	arg1	analyses					1159:1166	phylogenetic analyses	1146:1166	phylogenetic analyses	1146:1166	The results of phylogenetic analyses, in silico genomic comparisons, and chemotaxonomic and phenotypic analyses clearly indicated that strain 3-2-2T represents a novel species within the genus Bacillus, for which the name Bacillus acidinfaciens sp.
30747616	4	66	theme	draft	507:511	arg1	sequence					520:527	A draft genome sequence	505:527	A draft genome sequence determined for strain 3-2-2T	505:556	A draft genome sequence determined for strain 3-2-2T revealed a DNA G+C content of 42.2 mol%.
30747616	4	67	theme	strain	544:549	arg1	3-2-2T					551:556	strain 3-2-2T	544:556	strain 3-2-2T	544:556	A draft genome sequence determined for strain 3-2-2T revealed a DNA G+C content of 42.2 mol%.
30747616	4	68	theme	%	596:596	arg1	G+C content					573:583	a DNA G+C content	567:583	a DNA G+C content of 42.2 mol%	567:596	A draft genome sequence determined for strain 3-2-2T revealed a DNA G+C content of 42.2 mol%.
30747616	6	69	dep	iso-C15 	808:815	arg1	 0					847:848	 0	847:848	 0	847:848	The major fatty acids of strain 3-2-2T were iso-C15 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C16 : 0.
30747616	6	69	dep	iso-C15 	808:815	arg1	 0					834:835	 0	834:835	 0	834:835	The major fatty acids of strain 3-2-2T were iso-C15 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C16 : 0.
30747616	6	69	dep	iso-C15 	808:815	arg1	 0					817:818	 0	817:818	 0	817:818	The major fatty acids of strain 3-2-2T were iso-C15 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C16 : 0.
30747616	6	69	dep	iso-C15 	808:815	arg1	 0					863:864	 0	863:864	 0	863:864	The major fatty acids of strain 3-2-2T were iso-C15 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C16 : 0.
30747616	6	69	dep	iso-C15 	808:815	arg1	iso-C16 					854:861	iso-C16 	854:861	iso-C16 	854:861	The major fatty acids of strain 3-2-2T were iso-C15 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C16 : 0.
30747616	10	70	dep	name	1348:1351	arg1	sp					1376:1377	Bacillus acidinfaciens sp	1353:1377	the name Bacillus acidinfaciens sp	1344:1377	The results of phylogenetic analyses, in silico genomic comparisons, and chemotaxonomic and phenotypic analyses clearly indicated that strain 3-2-2T represents a novel species within the genus Bacillus, for which the name Bacillus acidinfaciens sp.
30747616	5	71	theme	average	603:609	arg1	identity					622:629	average nucleotide identity	603:629	average nucleotide identity	603:629	The average nucleotide identity and digital DNA-DNA hybridization values between 3-2-2T and the closely related Bacillus species ranged 79.4-84.2 % and 23.4-24.6 %.
30747616	10	72	theme	novel	1293:1297	arg1	species					1299:1305	a novel species	1291:1305	a novel species	1291:1305	The results of phylogenetic analyses, in silico genomic comparisons, and chemotaxonomic and phenotypic analyses clearly indicated that strain 3-2-2T represents a novel species within the genus Bacillus, for which the name Bacillus acidinfaciens sp.
30747616	4	73	theme	42.2 mol	588:595	arg1	%					596:596	42.2 mol%	588:596	42.2 mol%	588:596	A draft genome sequence determined for strain 3-2-2T revealed a DNA G+C content of 42.2 mol%.
30747616	10	74	theme	comparisons	1187:1197	arg1	results					1135:1141	The results	1131:1141	The results of phylogenetic analyses, in silico genomic comparisons, and chemotaxonomic and phenotypic analyses	1131:1241	The results of phylogenetic analyses, in silico genomic comparisons, and chemotaxonomic and phenotypic analyses clearly indicated that strain 3-2-2T represents a novel species within the genus Bacillus, for which the name Bacillus acidinfaciens sp.
30672727	9	0	theme	rRNA	887:890	arg1	gene					892:895	The 16S rRNA gene	879:895	The 16S rRNA gene	879:895	The 16S rRNA gene based phylogenetic analysis revealed that the facultative anaerobic strains B16-10T and Z23-18 showed the highest similarities to the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %).
30672727	5	1	theme	diagnostic	640:649	arg1	diaminoacid					651:661	diagnostic diaminoacid	640:661	diagnostic diaminoacid of the peptidoglycan	640:682	Both strains contained meso-diaminopimelic acid as diagnostic diaminoacid of the peptidoglycan.
30672727	3	2	theme	ellipsoidal	464:474	arg1	endospores					476:485	terminal, ellipsoidal endospores	454:485	endospores	476:485	Cells of both strains stained Gram-positive, were motile straight rods, and formed terminal, ellipsoidal endospores with swollen sporangia.
30672727	2	3	from	pH	211:212	arg1	NaCl					237:240	5 % (w/v) NaCl	227:240	5 % (w/v) NaCl	227:240	Two alkaliphilic and moderately halophilic bacterial strains B16-10T and Z23-18 characterized by optimal growth at pH 9.0-10.0 and 5 % (w/v) NaCl, were isolated from the rhizosphere soil of the bayonet grass (Bolboschoenus maritimus) in the Kiskunság National Park, Hungary.
30672727	2	3	from	pH	211:212	arg1	growth					201:206	optimal growth	193:206	optimal growth at pH 9.0-10.0	193:221	Two alkaliphilic and moderately halophilic bacterial strains B16-10T and Z23-18 characterized by optimal growth at pH 9.0-10.0 and 5 % (w/v) NaCl, were isolated from the rhizosphere soil of the bayonet grass (Bolboschoenus maritimus) in the Kiskunság National Park, Hungary.
30672727	2	4	from	grass	298:302	arg1	Hungary					362:368	Hungary	362:368	Hungary	362:368	Two alkaliphilic and moderately halophilic bacterial strains B16-10T and Z23-18 characterized by optimal growth at pH 9.0-10.0 and 5 % (w/v) NaCl, were isolated from the rhizosphere soil of the bayonet grass (Bolboschoenus maritimus) in the Kiskunság National Park, Hungary.
30672727	5	5	contain	contained	602:610	arg1	strains					594:600	Both strains	589:600	Both strains	589:600	Both strains contained meso-diaminopimelic acid as diagnostic diaminoacid of the peptidoglycan.
30672727	5	5	contain	contained	602:610	arg2	acid					632:635	meso-diaminopimelic acid	612:635	meso-diaminopimelic acid	612:635	Both strains contained meso-diaminopimelic acid as diagnostic diaminoacid of the peptidoglycan.
30672727	7	6	theme	fatty	815:819	arg1	acids					821:825	the major cellular fatty acids	796:825	the major cellular fatty acids	796:825	Anteiso-C15 : 0, C16 : 1ω11c and iso-C14 : 0 were the major cellular fatty acids.
30672727	7	6	theme	fatty	815:819	arg1	Anteiso-C15 					746:757	Anteiso-C15 	746:757	Anteiso-C15 : 0, C16 : 1ω11c and iso-C14 : 0	746:789	Anteiso-C15 : 0, C16 : 1ω11c and iso-C14 : 0 were the major cellular fatty acids.
30672727	7	7	theme	major	800:804	arg1	acids					821:825	the major cellular fatty acids	796:825	the major cellular fatty acids	796:825	Anteiso-C15 : 0, C16 : 1ω11c and iso-C14 : 0 were the major cellular fatty acids.
30672727	7	7	theme	major	800:804	arg1	Anteiso-C15 					746:757	Anteiso-C15 	746:757	Anteiso-C15 : 0, C16 : 1ω11c and iso-C14 : 0	746:789	Anteiso-C15 : 0, C16 : 1ω11c and iso-C14 : 0 were the major cellular fatty acids.
30672727	9	8	theme	facultative	943:953	arg1	strains					965:971	the facultative anaerobic strains	939:971	the facultative anaerobic strains B16-10T and Z23-18	939:990	The 16S rRNA gene based phylogenetic analysis revealed that the facultative anaerobic strains B16-10T and Z23-18 showed the highest similarities to the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %).
30672727	9	8	theme	facultative	943:953	arg1	Z23-18					985:990	Z23-18	985:990	Z23-18	985:990	The 16S rRNA gene based phylogenetic analysis revealed that the facultative anaerobic strains B16-10T and Z23-18 showed the highest similarities to the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %).
30672727	9	8	theme	facultative	943:953	arg1	B16-10T					973:979	B16-10T	973:979	B16-10T	973:979	The 16S rRNA gene based phylogenetic analysis revealed that the facultative anaerobic strains B16-10T and Z23-18 showed the highest similarities to the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %).
30672727	2	9	theme	alkaliphilic	100:111	arg1	B16-10T					157:163	B16-10T	157:163	B16-10T	157:163	Two alkaliphilic and moderately halophilic bacterial strains B16-10T and Z23-18 characterized by optimal growth at pH 9.0-10.0 and 5 % (w/v) NaCl, were isolated from the rhizosphere soil of the bayonet grass (Bolboschoenus maritimus) in the Kiskunság National Park, Hungary.
30672727	2	9	theme	alkaliphilic	100:111	arg1	strains					149:155	Two alkaliphilic and moderately halophilic bacterial strains B16-10T and Z23-18	96:174	Two alkaliphilic and moderately halophilic bacterial strains B16-10T and Z23-18	96:174	Two alkaliphilic and moderately halophilic bacterial strains B16-10T and Z23-18 characterized by optimal growth at pH 9.0-10.0 and 5 % (w/v) NaCl, were isolated from the rhizosphere soil of the bayonet grass (Bolboschoenus maritimus) in the Kiskunság National Park, Hungary.
30672727	2	9	theme	alkaliphilic	100:111	arg1	Z23-18					169:174	Z23-18	169:174	Z23-18	169:174	Two alkaliphilic and moderately halophilic bacterial strains B16-10T and Z23-18 characterized by optimal growth at pH 9.0-10.0 and 5 % (w/v) NaCl, were isolated from the rhizosphere soil of the bayonet grass (Bolboschoenus maritimus) in the Kiskunság National Park, Hungary.
30672727	9	10	theme	6T	1179:1180	arg1	strains					1036:1042	the type strains	1027:1042	the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %)	1027:1298	The 16S rRNA gene based phylogenetic analysis revealed that the facultative anaerobic strains B16-10T and Z23-18 showed the highest similarities to the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %).
30672727	5	11	theme	peptidoglycan	670:682	arg1	diaminoacid					651:661	diagnostic diaminoacid	640:661	diagnostic diaminoacid of the peptidoglycan	640:682	Both strains contained meso-diaminopimelic acid as diagnostic diaminoacid of the peptidoglycan.
30672727	9	12	theme	97.7	1183:1186	arg1	6T					1179:1180	A. alkalidiazotrophicus MS 6T	1152:1180	A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %)	1152:1198	The 16S rRNA gene based phylogenetic analysis revealed that the facultative anaerobic strains B16-10T and Z23-18 showed the highest similarities to the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %).
30672727	9	12	theme	97.7	1183:1186	arg1	%					1197:1197	97.7 and 98.4 %	1183:1197	%	1197:1197	The 16S rRNA gene based phylogenetic analysis revealed that the facultative anaerobic strains B16-10T and Z23-18 showed the highest similarities to the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %).
30672727	2	13	theme	5 	227:228	arg1	NaCl					237:240	5 % (w/v) NaCl	227:240	5 % (w/v) NaCl	227:240	Two alkaliphilic and moderately halophilic bacterial strains B16-10T and Z23-18 characterized by optimal growth at pH 9.0-10.0 and 5 % (w/v) NaCl, were isolated from the rhizosphere soil of the bayonet grass (Bolboschoenus maritimus) in the Kiskunság National Park, Hungary.
30672727	12	14	theme	29790T=NCAIM	1542:1553	arg1	02608T					1557:1562	=DSM 29790T=NCAIM B 02608T	1537:1562	=DSM 29790T=NCAIM B 02608T	1537:1562	The type strain is B16-10T (=DSM 29790T=NCAIM B 02608T).
30672727	12	14	theme	29790T=NCAIM	1542:1553	arg1	B16-10T					1528:1534	B16-10T	1528:1534	B16-10T (=DSM 29790T=NCAIM B 02608T)	1528:1563	The type strain is B16-10T (=DSM 29790T=NCAIM B 02608T).
30672727	9	15	theme	98.4 	1192:1196	arg1	6T					1179:1180	A. alkalidiazotrophicus MS 6T	1152:1180	A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %)	1152:1198	The 16S rRNA gene based phylogenetic analysis revealed that the facultative anaerobic strains B16-10T and Z23-18 showed the highest similarities to the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %).
30672727	9	15	theme	98.4 	1192:1196	arg1	%					1197:1197	97.7 and 98.4 %	1183:1197	%	1197:1197	The 16S rRNA gene based phylogenetic analysis revealed that the facultative anaerobic strains B16-10T and Z23-18 showed the highest similarities to the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %).
30672727	9	16	theme	highest	1003:1009	arg1	similarities					1011:1022	the highest similarities	999:1022	the highest similarities to the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %)	999:1298	The 16S rRNA gene based phylogenetic analysis revealed that the facultative anaerobic strains B16-10T and Z23-18 showed the highest similarities to the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %).
30672727	10	17	dep	strains	1386:1392	arg1	B16-10T					1394:1400	B16-10T	1394:1400	B16-10T	1394:1400	However, the distinctive phenotypic and genetic results of this study confirmed that strains B16-10T and Z23-18 represent a novel species, for which the name Anaerobacillus alkaliphilus sp.
30672727	10	17	dep	strains	1386:1392	arg1	strains					1386:1392	strains	1386:1392	strains B16-10T and Z23-18	1386:1411	However, the distinctive phenotypic and genetic results of this study confirmed that strains B16-10T and Z23-18 represent a novel species, for which the name Anaerobacillus alkaliphilus sp.
30672727	10	17	dep	strains	1386:1392	arg1	Z23-18					1406:1411	Z23-18	1406:1411	Z23-18	1406:1411	However, the distinctive phenotypic and genetic results of this study confirmed that strains B16-10T and Z23-18 represent a novel species, for which the name Anaerobacillus alkaliphilus sp.
30672727	12	18	theme	B	1555:1555	arg1	02608T					1557:1562	=DSM 29790T=NCAIM B 02608T	1537:1562	=DSM 29790T=NCAIM B 02608T	1537:1562	The type strain is B16-10T (=DSM 29790T=NCAIM B 02608T).
30672727	12	18	theme	B	1555:1555	arg1	B16-10T					1528:1534	B16-10T	1528:1534	B16-10T (=DSM 29790T=NCAIM B 02608T)	1528:1563	The type strain is B16-10T (=DSM 29790T=NCAIM B 02608T).
30672727	10	19	theme	phenotypic	1326:1335	arg1	results					1349:1355	the distinctive phenotypic and genetic results	1310:1355	the distinctive phenotypic and genetic results of this study	1310:1369	However, the distinctive phenotypic and genetic results of this study confirmed that strains B16-10T and Z23-18 represent a novel species, for which the name Anaerobacillus alkaliphilus sp.
30672727	1	20	dep	bacterium	85:93	arg1	nov.					32:35	nov.	32:35	nov.	32:35	nov., a novel alkaliphilic and moderately halophilic bacterium.
30672727	9	21	theme	type	1031:1034	arg1	strains					1036:1042	the type strains	1027:1042	the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %)	1027:1298	The 16S rRNA gene based phylogenetic analysis revealed that the facultative anaerobic strains B16-10T and Z23-18 showed the highest similarities to the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %).
30672727	9	22	theme	Z-0521T	1220:1226	arg1	strains					1036:1042	the type strains	1027:1042	the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %)	1027:1298	The 16S rRNA gene based phylogenetic analysis revealed that the facultative anaerobic strains B16-10T and Z23-18 showed the highest similarities to the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %).
30672727	9	23	theme	97.5	1229:1232	arg1	%					1243:1243	97.5 and 98.3 %	1229:1243	%	1243:1243	The 16S rRNA gene based phylogenetic analysis revealed that the facultative anaerobic strains B16-10T and Z23-18 showed the highest similarities to the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %).
30672727	9	23	theme	97.5	1229:1232	arg1	Z-0521T					1220:1226	Z-0521T	1220:1226	Z-0521T	1220:1226	The 16S rRNA gene based phylogenetic analysis revealed that the facultative anaerobic strains B16-10T and Z23-18 showed the highest similarities to the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %).
30672727	1	24	theme	halophilic	74:83	arg1	bacterium					85:93	a novel alkaliphilic and moderately halophilic bacterium	38:93	a novel alkaliphilic and moderately halophilic bacterium	38:93	nov., a novel alkaliphilic and moderately halophilic bacterium.
30672727	12	25	theme	=DSM	1537:1540	arg1	02608T					1557:1562	=DSM 29790T=NCAIM B 02608T	1537:1562	=DSM 29790T=NCAIM B 02608T	1537:1562	The type strain is B16-10T (=DSM 29790T=NCAIM B 02608T).
30672727	12	25	theme	=DSM	1537:1540	arg1	B16-10T					1528:1534	B16-10T	1528:1534	B16-10T (=DSM 29790T=NCAIM B 02608T)	1528:1563	The type strain is B16-10T (=DSM 29790T=NCAIM B 02608T).
30672727	10	26	theme	genetic	1341:1347	arg1	results					1349:1355	the distinctive phenotypic and genetic results	1310:1355	the distinctive phenotypic and genetic results of this study	1310:1369	However, the distinctive phenotypic and genetic results of this study confirmed that strains B16-10T and Z23-18 represent a novel species, for which the name Anaerobacillus alkaliphilus sp.
30672727	9	27	theme	Anaerobacillus	1057:1070	arg1	%					1112:1112	98.7 and 99.1 %	1098:1112	%	1112:1112	The 16S rRNA gene based phylogenetic analysis revealed that the facultative anaerobic strains B16-10T and Z23-18 showed the highest similarities to the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %).
30672727	9	27	theme	Anaerobacillus	1057:1070	arg1	NB2006T					1089:1095	anaerobic Anaerobacillus isosaccharinicus NB2006T	1047:1095	anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %)	1047:1113	The 16S rRNA gene based phylogenetic analysis revealed that the facultative anaerobic strains B16-10T and Z23-18 showed the highest similarities to the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %).
30672727	3	28	theme	strains	385:391	arg1	Cells					371:375	Cells	371:375	Cells of both strains	371:391	Cells of both strains stained Gram-positive, were motile straight rods, and formed terminal, ellipsoidal endospores with swollen sporangia.
30672727	0	29	theme	Anaerobacillus	0:13	arg1	sp					28:29	Anaerobacillus alkaliphilus sp	0:29	Anaerobacillus alkaliphilus sp.	0:30	Anaerobacillus alkaliphilus sp.
30672727	9	30	theme	98.3 	1238:1242	arg1	%					1243:1243	97.5 and 98.3 %	1229:1243	%	1243:1243	The 16S rRNA gene based phylogenetic analysis revealed that the facultative anaerobic strains B16-10T and Z23-18 showed the highest similarities to the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %).
30672727	9	30	theme	98.3 	1238:1242	arg1	Z-0521T					1220:1226	Z-0521T	1220:1226	Z-0521T	1220:1226	The 16S rRNA gene based phylogenetic analysis revealed that the facultative anaerobic strains B16-10T and Z23-18 showed the highest similarities to the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %).
30672727	9	31	dep	strains	965:971	arg1	strains					965:971	the facultative anaerobic strains	939:971	the facultative anaerobic strains B16-10T and Z23-18	939:990	The 16S rRNA gene based phylogenetic analysis revealed that the facultative anaerobic strains B16-10T and Z23-18 showed the highest similarities to the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %).
30672727	9	31	dep	strains	965:971	arg1	Z23-18					985:990	Z23-18	985:990	Z23-18	985:990	The 16S rRNA gene based phylogenetic analysis revealed that the facultative anaerobic strains B16-10T and Z23-18 showed the highest similarities to the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %).
30672727	9	31	dep	strains	965:971	arg1	B16-10T					973:979	B16-10T	973:979	B16-10T	973:979	The 16S rRNA gene based phylogenetic analysis revealed that the facultative anaerobic strains B16-10T and Z23-18 showed the highest similarities to the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %).
30672727	2	32	dep	strains	149:155	arg1	B16-10T					157:163	B16-10T	157:163	B16-10T	157:163	Two alkaliphilic and moderately halophilic bacterial strains B16-10T and Z23-18 characterized by optimal growth at pH 9.0-10.0 and 5 % (w/v) NaCl, were isolated from the rhizosphere soil of the bayonet grass (Bolboschoenus maritimus) in the Kiskunság National Park, Hungary.
30672727	2	32	dep	strains	149:155	arg1	strains					149:155	Two alkaliphilic and moderately halophilic bacterial strains B16-10T and Z23-18	96:174	Two alkaliphilic and moderately halophilic bacterial strains B16-10T and Z23-18	96:174	Two alkaliphilic and moderately halophilic bacterial strains B16-10T and Z23-18 characterized by optimal growth at pH 9.0-10.0 and 5 % (w/v) NaCl, were isolated from the rhizosphere soil of the bayonet grass (Bolboschoenus maritimus) in the Kiskunság National Park, Hungary.
30672727	2	32	dep	strains	149:155	arg1	Z23-18					169:174	Z23-18	169:174	Z23-18	169:174	Two alkaliphilic and moderately halophilic bacterial strains B16-10T and Z23-18 characterized by optimal growth at pH 9.0-10.0 and 5 % (w/v) NaCl, were isolated from the rhizosphere soil of the bayonet grass (Bolboschoenus maritimus) in the Kiskunság National Park, Hungary.
30672727	9	33	theme	NB2006T	1089:1095	arg1	strains					1036:1042	the type strains	1027:1042	the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %)	1027:1298	The 16S rRNA gene based phylogenetic analysis revealed that the facultative anaerobic strains B16-10T and Z23-18 showed the highest similarities to the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %).
30672727	3	34	theme	straight	428:435	arg1	rods					437:440	motile straight rods	421:440	motile straight rods	421:440	Cells of both strains stained Gram-positive, were motile straight rods, and formed terminal, ellipsoidal endospores with swollen sporangia.
30672727	12	35	theme	type	1513:1516	arg1	B16-10T					1528:1534	B16-10T	1528:1534	B16-10T (=DSM 29790T=NCAIM B 02608T)	1528:1563	The type strain is B16-10T (=DSM 29790T=NCAIM B 02608T).
30672727	12	35	theme	type	1513:1516	arg1	strain					1518:1523	The type strain	1509:1523	The type strain	1509:1523	The type strain is B16-10T (=DSM 29790T=NCAIM B 02608T).
30672727	10	36	theme	study	1365:1369	arg1	results					1349:1355	the distinctive phenotypic and genetic results	1310:1355	the distinctive phenotypic and genetic results of this study	1310:1369	However, the distinctive phenotypic and genetic results of this study confirmed that strains B16-10T and Z23-18 represent a novel species, for which the name Anaerobacillus alkaliphilus sp.
30672727	2	37	theme	optimal	193:199	arg1	growth					201:206	optimal growth	193:206	optimal growth at pH 9.0-10.0	193:221	Two alkaliphilic and moderately halophilic bacterial strains B16-10T and Z23-18 characterized by optimal growth at pH 9.0-10.0 and 5 % (w/v) NaCl, were isolated from the rhizosphere soil of the bayonet grass (Bolboschoenus maritimus) in the Kiskunság National Park, Hungary.
30672727	9	38	theme	98.7	1098:1101	arg1	%					1112:1112	98.7 and 99.1 %	1098:1112	%	1112:1112	The 16S rRNA gene based phylogenetic analysis revealed that the facultative anaerobic strains B16-10T and Z23-18 showed the highest similarities to the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %).
30672727	9	38	theme	98.7	1098:1101	arg1	NB2006T					1089:1095	anaerobic Anaerobacillus isosaccharinicus NB2006T	1047:1095	anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %)	1047:1113	The 16S rRNA gene based phylogenetic analysis revealed that the facultative anaerobic strains B16-10T and Z23-18 showed the highest similarities to the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %).
30672727	9	39	theme	A.	1250:1251	arg1	%					1297:1297	97.5 and 98.2 %	1283:1297	%	1297:1297	The 16S rRNA gene based phylogenetic analysis revealed that the facultative anaerobic strains B16-10T and Z23-18 showed the highest similarities to the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %).
30672727	9	39	theme	A.	1250:1251	arg1	15340T					1275:1280	A. arseniciselenatis DSM 15340T	1250:1280	A. arseniciselenatis DSM 15340T (97.5 and 98.2 %)	1250:1298	The 16S rRNA gene based phylogenetic analysis revealed that the facultative anaerobic strains B16-10T and Z23-18 showed the highest similarities to the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %).
30672727	9	40	theme	99.1 	1107:1111	arg1	%					1112:1112	98.7 and 99.1 %	1098:1112	%	1112:1112	The 16S rRNA gene based phylogenetic analysis revealed that the facultative anaerobic strains B16-10T and Z23-18 showed the highest similarities to the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %).
30672727	9	40	theme	99.1 	1107:1111	arg1	NB2006T					1089:1095	anaerobic Anaerobacillus isosaccharinicus NB2006T	1047:1095	anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %)	1047:1113	The 16S rRNA gene based phylogenetic analysis revealed that the facultative anaerobic strains B16-10T and Z23-18 showed the highest similarities to the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %).
30672727	9	41	theme	DSM	1271:1273	arg1	%					1297:1297	97.5 and 98.2 %	1283:1297	%	1297:1297	The 16S rRNA gene based phylogenetic analysis revealed that the facultative anaerobic strains B16-10T and Z23-18 showed the highest similarities to the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %).
30672727	9	41	theme	DSM	1271:1273	arg1	15340T					1275:1280	A. arseniciselenatis DSM 15340T	1250:1280	A. arseniciselenatis DSM 15340T (97.5 and 98.2 %)	1250:1298	The 16S rRNA gene based phylogenetic analysis revealed that the facultative anaerobic strains B16-10T and Z23-18 showed the highest similarities to the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %).
30672727	2	42	from	Hungary	362:368	arg1	soil					278:281	the rhizosphere soil	262:281	the rhizosphere soil of the bayonet grass (Bolboschoenus maritimus) in the Kiskunság National Park, Hungary	262:368	Two alkaliphilic and moderately halophilic bacterial strains B16-10T and Z23-18 characterized by optimal growth at pH 9.0-10.0 and 5 % (w/v) NaCl, were isolated from the rhizosphere soil of the bayonet grass (Bolboschoenus maritimus) in the Kiskunság National Park, Hungary.
30672727	9	43	theme	A.	1116:1117	arg1	%					1148:1148	98.2 and 98.4 %	1134:1148	%	1148:1148	The 16S rRNA gene based phylogenetic analysis revealed that the facultative anaerobic strains B16-10T and Z23-18 showed the highest similarities to the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %).
30672727	9	43	theme	A.	1116:1117	arg1	JMM-4T					1126:1131	A. macyae JMM-4T	1116:1131	A. macyae JMM-4T (98.2 and 98.4 %)	1116:1149	The 16S rRNA gene based phylogenetic analysis revealed that the facultative anaerobic strains B16-10T and Z23-18 showed the highest similarities to the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %).
30672727	1	44	theme	novel	40:44	arg1	bacterium					85:93	a novel alkaliphilic and moderately halophilic bacterium	38:93	a novel alkaliphilic and moderately halophilic bacterium	38:93	nov., a novel alkaliphilic and moderately halophilic bacterium.
30672727	9	45	theme	16S	883:885	arg1	gene					892:895	The 16S rRNA gene	879:895	The 16S rRNA gene	879:895	The 16S rRNA gene based phylogenetic analysis revealed that the facultative anaerobic strains B16-10T and Z23-18 showed the highest similarities to the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %).
30672727	2	46	theme	halophilic	128:137	arg1	B16-10T					157:163	B16-10T	157:163	B16-10T	157:163	Two alkaliphilic and moderately halophilic bacterial strains B16-10T and Z23-18 characterized by optimal growth at pH 9.0-10.0 and 5 % (w/v) NaCl, were isolated from the rhizosphere soil of the bayonet grass (Bolboschoenus maritimus) in the Kiskunság National Park, Hungary.
30672727	2	46	theme	halophilic	128:137	arg1	strains					149:155	Two alkaliphilic and moderately halophilic bacterial strains B16-10T and Z23-18	96:174	Two alkaliphilic and moderately halophilic bacterial strains B16-10T and Z23-18	96:174	Two alkaliphilic and moderately halophilic bacterial strains B16-10T and Z23-18 characterized by optimal growth at pH 9.0-10.0 and 5 % (w/v) NaCl, were isolated from the rhizosphere soil of the bayonet grass (Bolboschoenus maritimus) in the Kiskunság National Park, Hungary.
30672727	2	46	theme	halophilic	128:137	arg1	Z23-18					169:174	Z23-18	169:174	Z23-18	169:174	Two alkaliphilic and moderately halophilic bacterial strains B16-10T and Z23-18 characterized by optimal growth at pH 9.0-10.0 and 5 % (w/v) NaCl, were isolated from the rhizosphere soil of the bayonet grass (Bolboschoenus maritimus) in the Kiskunság National Park, Hungary.
30672727	9	47	theme	JMM-4T	1126:1131	arg1	strains					1036:1042	the type strains	1027:1042	the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %)	1027:1298	The 16S rRNA gene based phylogenetic analysis revealed that the facultative anaerobic strains B16-10T and Z23-18 showed the highest similarities to the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %).
30672727	9	48	theme	98.2	1134:1137	arg1	%					1148:1148	98.2 and 98.4 %	1134:1148	%	1148:1148	The 16S rRNA gene based phylogenetic analysis revealed that the facultative anaerobic strains B16-10T and Z23-18 showed the highest similarities to the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %).
30672727	9	48	theme	98.2	1134:1137	arg1	JMM-4T					1126:1131	A. macyae JMM-4T	1116:1131	A. macyae JMM-4T (98.2 and 98.4 %)	1116:1149	The 16S rRNA gene based phylogenetic analysis revealed that the facultative anaerobic strains B16-10T and Z23-18 showed the highest similarities to the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %).
30672727	2	49	theme	rhizosphere	266:276	arg1	soil					278:281	the rhizosphere soil	262:281	the rhizosphere soil of the bayonet grass (Bolboschoenus maritimus) in the Kiskunság National Park, Hungary	262:368	Two alkaliphilic and moderately halophilic bacterial strains B16-10T and Z23-18 characterized by optimal growth at pH 9.0-10.0 and 5 % (w/v) NaCl, were isolated from the rhizosphere soil of the bayonet grass (Bolboschoenus maritimus) in the Kiskunság National Park, Hungary.
30672727	9	50	dep	A.	1152:1153	arg1	alkalidiazotrophicus					1155:1174	alkalidiazotrophicus	1155:1174	alkalidiazotrophicus	1155:1174	The 16S rRNA gene based phylogenetic analysis revealed that the facultative anaerobic strains B16-10T and Z23-18 showed the highest similarities to the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %).
30672727	9	51	theme	phylogenetic	903:914	arg1	analysis					916:923	phylogenetic analysis	903:923	phylogenetic analysis	903:923	The 16S rRNA gene based phylogenetic analysis revealed that the facultative anaerobic strains B16-10T and Z23-18 showed the highest similarities to the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %).
30672727	10	52	dep	Anaerobacillus	1459:1472	arg1	alkaliphilus					1474:1485	alkaliphilus	1474:1485	alkaliphilus	1474:1485	However, the distinctive phenotypic and genetic results of this study confirmed that strains B16-10T and Z23-18 represent a novel species, for which the name Anaerobacillus alkaliphilus sp.
30672727	9	53	theme	98.4 	1143:1147	arg1	%					1148:1148	98.2 and 98.4 %	1134:1148	%	1148:1148	The 16S rRNA gene based phylogenetic analysis revealed that the facultative anaerobic strains B16-10T and Z23-18 showed the highest similarities to the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %).
30672727	9	53	theme	98.4 	1143:1147	arg1	JMM-4T					1126:1131	A. macyae JMM-4T	1116:1131	A. macyae JMM-4T (98.2 and 98.4 %)	1116:1149	The 16S rRNA gene based phylogenetic analysis revealed that the facultative anaerobic strains B16-10T and Z23-18 showed the highest similarities to the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %).
30672727	3	54	theme	swollen	492:498	arg1	sporangia					500:508	swollen sporangia	492:508	swollen sporangia	492:508	Cells of both strains stained Gram-positive, were motile straight rods, and formed terminal, ellipsoidal endospores with swollen sporangia.
30672727	5	55	theme	meso-diaminopimelic	612:630	arg1	acid					632:635	meso-diaminopimelic acid	612:635	meso-diaminopimelic acid	612:635	Both strains contained meso-diaminopimelic acid as diagnostic diaminoacid of the peptidoglycan.
30672727	2	56	theme	bayonet	290:296	arg1	grass					298:302	the bayonet grass	286:302	the bayonet grass (Bolboschoenus maritimus) in the Kiskunság National Park, Hungary	286:368	Two alkaliphilic and moderately halophilic bacterial strains B16-10T and Z23-18 characterized by optimal growth at pH 9.0-10.0 and 5 % (w/v) NaCl, were isolated from the rhizosphere soil of the bayonet grass (Bolboschoenus maritimus) in the Kiskunság National Park, Hungary.
30672727	10	57	theme	Anaerobacillus	1459:1472	arg1	sp					1487:1488	the name Anaerobacillus alkaliphilus sp	1450:1488	the name Anaerobacillus alkaliphilus sp	1450:1488	However, the distinctive phenotypic and genetic results of this study confirmed that strains B16-10T and Z23-18 represent a novel species, for which the name Anaerobacillus alkaliphilus sp.
30672727	9	58	theme	A.	1152:1153	arg1	6T					1179:1180	A. alkalidiazotrophicus MS 6T	1152:1180	A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %)	1152:1198	The 16S rRNA gene based phylogenetic analysis revealed that the facultative anaerobic strains B16-10T and Z23-18 showed the highest similarities to the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %).
30672727	9	58	theme	A.	1152:1153	arg1	%					1197:1197	97.7 and 98.4 %	1183:1197	%	1197:1197	The 16S rRNA gene based phylogenetic analysis revealed that the facultative anaerobic strains B16-10T and Z23-18 showed the highest similarities to the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %).
30672727	7	59	theme	cellular	806:813	arg1	acids					821:825	the major cellular fatty acids	796:825	the major cellular fatty acids	796:825	Anteiso-C15 : 0, C16 : 1ω11c and iso-C14 : 0 were the major cellular fatty acids.
30672727	7	59	theme	cellular	806:813	arg1	Anteiso-C15 					746:757	Anteiso-C15 	746:757	Anteiso-C15 : 0, C16 : 1ω11c and iso-C14 : 0	746:789	Anteiso-C15 : 0, C16 : 1ω11c and iso-C14 : 0 were the major cellular fatty acids.
30672727	9	60	theme	anaerobic	955:963	arg1	strains					965:971	the facultative anaerobic strains	939:971	the facultative anaerobic strains B16-10T and Z23-18	939:990	The 16S rRNA gene based phylogenetic analysis revealed that the facultative anaerobic strains B16-10T and Z23-18 showed the highest similarities to the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %).
30672727	9	60	theme	anaerobic	955:963	arg1	Z23-18					985:990	Z23-18	985:990	Z23-18	985:990	The 16S rRNA gene based phylogenetic analysis revealed that the facultative anaerobic strains B16-10T and Z23-18 showed the highest similarities to the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %).
30672727	9	60	theme	anaerobic	955:963	arg1	B16-10T					973:979	B16-10T	973:979	B16-10T	973:979	The 16S rRNA gene based phylogenetic analysis revealed that the facultative anaerobic strains B16-10T and Z23-18 showed the highest similarities to the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %).
30672727	9	61	theme	MS	1176:1177	arg1	6T					1179:1180	A. alkalidiazotrophicus MS 6T	1152:1180	A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %)	1152:1198	The 16S rRNA gene based phylogenetic analysis revealed that the facultative anaerobic strains B16-10T and Z23-18 showed the highest similarities to the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %).
30672727	9	61	theme	MS	1176:1177	arg1	%					1197:1197	97.7 and 98.4 %	1183:1197	%	1197:1197	The 16S rRNA gene based phylogenetic analysis revealed that the facultative anaerobic strains B16-10T and Z23-18 showed the highest similarities to the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %).
30672727	2	62	theme	w/v	232:234	arg1	NaCl					237:240	5 % (w/v) NaCl	227:240	5 % (w/v) NaCl	227:240	Two alkaliphilic and moderately halophilic bacterial strains B16-10T and Z23-18 characterized by optimal growth at pH 9.0-10.0 and 5 % (w/v) NaCl, were isolated from the rhizosphere soil of the bayonet grass (Bolboschoenus maritimus) in the Kiskunság National Park, Hungary.
30672727	7	63	theme	C16 	763:766	arg1	Anteiso-C15 					746:757	Anteiso-C15 	746:757	Anteiso-C15 : 0, C16 : 1ω11c and iso-C14 : 0	746:789	Anteiso-C15 : 0, C16 : 1ω11c and iso-C14 : 0 were the major cellular fatty acids.
30672727	7	63	theme	C16 	763:766	arg1	acids					821:825	the major cellular fatty acids	796:825	the major cellular fatty acids	796:825	Anteiso-C15 : 0, C16 : 1ω11c and iso-C14 : 0 were the major cellular fatty acids.
30672727	2	64	theme	%	229:229	arg1	NaCl					237:240	5 % (w/v) NaCl	227:240	5 % (w/v) NaCl	227:240	Two alkaliphilic and moderately halophilic bacterial strains B16-10T and Z23-18 characterized by optimal growth at pH 9.0-10.0 and 5 % (w/v) NaCl, were isolated from the rhizosphere soil of the bayonet grass (Bolboschoenus maritimus) in the Kiskunság National Park, Hungary.
30672727	2	65	attach	isolated	248:255	arg2	Z23-18					169:174	Z23-18	169:174	Z23-18	169:174	Two alkaliphilic and moderately halophilic bacterial strains B16-10T and Z23-18 characterized by optimal growth at pH 9.0-10.0 and 5 % (w/v) NaCl, were isolated from the rhizosphere soil of the bayonet grass (Bolboschoenus maritimus) in the Kiskunság National Park, Hungary.
30672727	2	65	attach	isolated	248:255	arg2	B16-10T					157:163	B16-10T	157:163	B16-10T	157:163	Two alkaliphilic and moderately halophilic bacterial strains B16-10T and Z23-18 characterized by optimal growth at pH 9.0-10.0 and 5 % (w/v) NaCl, were isolated from the rhizosphere soil of the bayonet grass (Bolboschoenus maritimus) in the Kiskunság National Park, Hungary.
30672727	2	65	attach	isolated	248:255	arg2	strains					149:155	Two alkaliphilic and moderately halophilic bacterial strains B16-10T and Z23-18	96:174	Two alkaliphilic and moderately halophilic bacterial strains B16-10T and Z23-18	96:174	Two alkaliphilic and moderately halophilic bacterial strains B16-10T and Z23-18 characterized by optimal growth at pH 9.0-10.0 and 5 % (w/v) NaCl, were isolated from the rhizosphere soil of the bayonet grass (Bolboschoenus maritimus) in the Kiskunság National Park, Hungary.
30672727	2	65	attach	isolated	248:255	arg1	soil					278:281	the rhizosphere soil	262:281	the rhizosphere soil of the bayonet grass (Bolboschoenus maritimus) in the Kiskunság National Park, Hungary	262:368	Two alkaliphilic and moderately halophilic bacterial strains B16-10T and Z23-18 characterized by optimal growth at pH 9.0-10.0 and 5 % (w/v) NaCl, were isolated from the rhizosphere soil of the bayonet grass (Bolboschoenus maritimus) in the Kiskunság National Park, Hungary.
30672727	10	66	theme	name	1454:1457	arg1	sp					1487:1488	the name Anaerobacillus alkaliphilus sp	1450:1488	the name Anaerobacillus alkaliphilus sp	1450:1488	However, the distinctive phenotypic and genetic results of this study confirmed that strains B16-10T and Z23-18 represent a novel species, for which the name Anaerobacillus alkaliphilus sp.
30672727	2	67	from	soil	278:281	arg1	Hungary					362:368	Hungary	362:368	Hungary	362:368	Two alkaliphilic and moderately halophilic bacterial strains B16-10T and Z23-18 characterized by optimal growth at pH 9.0-10.0 and 5 % (w/v) NaCl, were isolated from the rhizosphere soil of the bayonet grass (Bolboschoenus maritimus) in the Kiskunság National Park, Hungary.
30672727	10	68	theme	distinctive	1314:1324	arg1	results					1349:1355	the distinctive phenotypic and genetic results	1310:1355	the distinctive phenotypic and genetic results of this study	1310:1369	However, the distinctive phenotypic and genetic results of this study confirmed that strains B16-10T and Z23-18 represent a novel species, for which the name Anaerobacillus alkaliphilus sp.
30672727	1	69	theme	alkaliphilic	46:57	arg1	bacterium					85:93	a novel alkaliphilic and moderately halophilic bacterium	38:93	a novel alkaliphilic and moderately halophilic bacterium	38:93	nov., a novel alkaliphilic and moderately halophilic bacterium.
30672727	8	70	theme	DNA	832:834	arg1	G+C content					836:846	The DNA G+C content	828:846	The DNA G+C content of both strains	828:862	The DNA G+C content of both strains was 35.8 mol%.
30672727	8	70	theme	DNA	832:834	arg1	%					876:876	35.8 mol%	868:876	35.8 mol%	868:876	The DNA G+C content of both strains was 35.8 mol%.
30672727	9	71	dep	A.	1250:1251	arg1	arseniciselenatis					1253:1269	arseniciselenatis	1253:1269	arseniciselenatis	1253:1269	The 16S rRNA gene based phylogenetic analysis revealed that the facultative anaerobic strains B16-10T and Z23-18 showed the highest similarities to the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %).
30672727	9	72	theme	anaerobic	1047:1055	arg1	%					1112:1112	98.7 and 99.1 %	1098:1112	%	1112:1112	The 16S rRNA gene based phylogenetic analysis revealed that the facultative anaerobic strains B16-10T and Z23-18 showed the highest similarities to the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %).
30672727	9	72	theme	anaerobic	1047:1055	arg1	NB2006T					1089:1095	anaerobic Anaerobacillus isosaccharinicus NB2006T	1047:1095	anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %)	1047:1113	The 16S rRNA gene based phylogenetic analysis revealed that the facultative anaerobic strains B16-10T and Z23-18 showed the highest similarities to the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %).
30672727	0	73	theme	alkaliphilus	15:26	arg1	sp					28:29	Anaerobacillus alkaliphilus sp	0:29	Anaerobacillus alkaliphilus sp.	0:30	Anaerobacillus alkaliphilus sp.
30672727	2	74	dep	grass	298:302	arg1	maritimus					319:327	Bolboschoenus maritimus	305:327	Bolboschoenus maritimus	305:327	Two alkaliphilic and moderately halophilic bacterial strains B16-10T and Z23-18 characterized by optimal growth at pH 9.0-10.0 and 5 % (w/v) NaCl, were isolated from the rhizosphere soil of the bayonet grass (Bolboschoenus maritimus) in the Kiskunság National Park, Hungary.
30672727	8	75	theme	strains	856:862	arg1	G+C content					836:846	The DNA G+C content	828:846	The DNA G+C content of both strains	828:862	The DNA G+C content of both strains was 35.8 mol%.
30672727	8	75	theme	strains	856:862	arg1	%					876:876	35.8 mol%	868:876	35.8 mol%	868:876	The DNA G+C content of both strains was 35.8 mol%.
30672727	6	76	theme	isoprenoid	726:735	arg1	quinone					737:743	the predominant isoprenoid quinone	710:743	the predominant isoprenoid quinone	710:743	Menaquinone-7 (MK-7) was the predominant isoprenoid quinone.
30672727	6	76	theme	isoprenoid	726:735	arg1	Menaquinone-7					685:697	Menaquinone-7	685:697	Menaquinone-7 (MK-7)	685:704	Menaquinone-7 (MK-7) was the predominant isoprenoid quinone.
30672727	7	77	theme	iso-C14 	779:786	arg1	Anteiso-C15 					746:757	Anteiso-C15 	746:757	Anteiso-C15 : 0, C16 : 1ω11c and iso-C14 : 0	746:789	Anteiso-C15 : 0, C16 : 1ω11c and iso-C14 : 0 were the major cellular fatty acids.
30672727	7	77	theme	iso-C14 	779:786	arg1	acids					821:825	the major cellular fatty acids	796:825	the major cellular fatty acids	796:825	Anteiso-C15 : 0, C16 : 1ω11c and iso-C14 : 0 were the major cellular fatty acids.
30672727	8	78	theme	35.8 mol	868:875	arg1	G+C content					836:846	The DNA G+C content	828:846	The DNA G+C content of both strains	828:862	The DNA G+C content of both strains was 35.8 mol%.
30672727	8	78	theme	35.8 mol	868:875	arg1	%					876:876	35.8 mol%	868:876	35.8 mol%	868:876	The DNA G+C content of both strains was 35.8 mol%.
30672727	3	79	theme	motile	421:426	arg1	rods					437:440	motile straight rods	421:440	motile straight rods	421:440	Cells of both strains stained Gram-positive, were motile straight rods, and formed terminal, ellipsoidal endospores with swollen sporangia.
30672727	7	80	theme	 1ω11c	768:773	arg1	Anteiso-C15 					746:757	Anteiso-C15 	746:757	Anteiso-C15 : 0, C16 : 1ω11c and iso-C14 : 0	746:789	Anteiso-C15 : 0, C16 : 1ω11c and iso-C14 : 0 were the major cellular fatty acids.
30672727	7	80	theme	 1ω11c	768:773	arg1	acids					821:825	the major cellular fatty acids	796:825	the major cellular fatty acids	796:825	Anteiso-C15 : 0, C16 : 1ω11c and iso-C14 : 0 were the major cellular fatty acids.
30672727	9	81	dep	Anaerobacillus	1057:1070	arg1	isosaccharinicus					1072:1087	isosaccharinicus	1072:1087	isosaccharinicus	1072:1087	The 16S rRNA gene based phylogenetic analysis revealed that the facultative anaerobic strains B16-10T and Z23-18 showed the highest similarities to the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %).
30672727	3	82	theme	terminal	454:461	arg1	endospores					476:485	terminal, ellipsoidal endospores	454:485	endospores	476:485	Cells of both strains stained Gram-positive, were motile straight rods, and formed terminal, ellipsoidal endospores with swollen sporangia.
30672727	9	83	theme	15340T	1275:1280	arg1	strains					1036:1042	the type strains	1027:1042	the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %)	1027:1298	The 16S rRNA gene based phylogenetic analysis revealed that the facultative anaerobic strains B16-10T and Z23-18 showed the highest similarities to the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %).
30672727	6	84	theme	predominant	714:724	arg1	quinone					737:743	the predominant isoprenoid quinone	710:743	the predominant isoprenoid quinone	710:743	Menaquinone-7 (MK-7) was the predominant isoprenoid quinone.
30672727	6	84	theme	predominant	714:724	arg1	Menaquinone-7					685:697	Menaquinone-7	685:697	Menaquinone-7 (MK-7)	685:704	Menaquinone-7 (MK-7) was the predominant isoprenoid quinone.
30672727	2	85	theme	grass	298:302	arg1	soil					278:281	the rhizosphere soil	262:281	the rhizosphere soil of the bayonet grass (Bolboschoenus maritimus) in the Kiskunság National Park, Hungary	262:368	Two alkaliphilic and moderately halophilic bacterial strains B16-10T and Z23-18 characterized by optimal growth at pH 9.0-10.0 and 5 % (w/v) NaCl, were isolated from the rhizosphere soil of the bayonet grass (Bolboschoenus maritimus) in the Kiskunság National Park, Hungary.
30672727	9	86	theme	97.5	1283:1286	arg1	%					1297:1297	97.5 and 98.2 %	1283:1297	%	1297:1297	The 16S rRNA gene based phylogenetic analysis revealed that the facultative anaerobic strains B16-10T and Z23-18 showed the highest similarities to the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %).
30672727	9	86	theme	97.5	1283:1286	arg1	15340T					1275:1280	A. arseniciselenatis DSM 15340T	1250:1280	A. arseniciselenatis DSM 15340T (97.5 and 98.2 %)	1250:1298	The 16S rRNA gene based phylogenetic analysis revealed that the facultative anaerobic strains B16-10T and Z23-18 showed the highest similarities to the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %).
30672727	10	87	theme	novel	1425:1429	arg1	species					1431:1437	a novel species	1423:1437	a novel species	1423:1437	However, the distinctive phenotypic and genetic results of this study confirmed that strains B16-10T and Z23-18 represent a novel species, for which the name Anaerobacillus alkaliphilus sp.
30672727	2	88	theme	bacterial	139:147	arg1	B16-10T					157:163	B16-10T	157:163	B16-10T	157:163	Two alkaliphilic and moderately halophilic bacterial strains B16-10T and Z23-18 characterized by optimal growth at pH 9.0-10.0 and 5 % (w/v) NaCl, were isolated from the rhizosphere soil of the bayonet grass (Bolboschoenus maritimus) in the Kiskunság National Park, Hungary.
30672727	2	88	theme	bacterial	139:147	arg1	strains					149:155	Two alkaliphilic and moderately halophilic bacterial strains B16-10T and Z23-18	96:174	Two alkaliphilic and moderately halophilic bacterial strains B16-10T and Z23-18	96:174	Two alkaliphilic and moderately halophilic bacterial strains B16-10T and Z23-18 characterized by optimal growth at pH 9.0-10.0 and 5 % (w/v) NaCl, were isolated from the rhizosphere soil of the bayonet grass (Bolboschoenus maritimus) in the Kiskunság National Park, Hungary.
30672727	2	88	theme	bacterial	139:147	arg1	Z23-18					169:174	Z23-18	169:174	Z23-18	169:174	Two alkaliphilic and moderately halophilic bacterial strains B16-10T and Z23-18 characterized by optimal growth at pH 9.0-10.0 and 5 % (w/v) NaCl, were isolated from the rhizosphere soil of the bayonet grass (Bolboschoenus maritimus) in the Kiskunság National Park, Hungary.
30672727	9	89	theme	macyae	1119:1124	arg1	%					1148:1148	98.2 and 98.4 %	1134:1148	%	1148:1148	The 16S rRNA gene based phylogenetic analysis revealed that the facultative anaerobic strains B16-10T and Z23-18 showed the highest similarities to the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %).
30672727	9	89	theme	macyae	1119:1124	arg1	JMM-4T					1126:1131	A. macyae JMM-4T	1116:1131	A. macyae JMM-4T (98.2 and 98.4 %)	1116:1149	The 16S rRNA gene based phylogenetic analysis revealed that the facultative anaerobic strains B16-10T and Z23-18 showed the highest similarities to the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %).
30672727	9	90	theme	98.2 	1292:1296	arg1	%					1297:1297	97.5 and 98.2 %	1283:1297	%	1297:1297	The 16S rRNA gene based phylogenetic analysis revealed that the facultative anaerobic strains B16-10T and Z23-18 showed the highest similarities to the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %).
30672727	9	90	theme	98.2 	1292:1296	arg1	15340T					1275:1280	A. arseniciselenatis DSM 15340T	1250:1280	A. arseniciselenatis DSM 15340T (97.5 and 98.2 %)	1250:1298	The 16S rRNA gene based phylogenetic analysis revealed that the facultative anaerobic strains B16-10T and Z23-18 showed the highest similarities to the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %).
31447165	3	0	theme	hay	761:763	arg1	silage					770:775	hay crop silage	761:775	6.3% hay crop silage	756:775	All diets contained 6.3% hay crop silage and 44.7% concentrate.
31447165	2	1	dep	hybrid	665:670	arg1	2					643:643	2	643:643	2	643:643	Cows were housed in tiestalls, milked 3 times/d, and fed a total mixed ration containing 49.0% (dry matter basis) of (1) a conventional corn silage hybrid (CONV); (2) a brown midrib bm3 hybrid (BMR); or (3) a bm3 hybrid with floury kernel genetics (BMRFL).
31447165	9	2	theme	protein	1415:1421	arg1	yield					1423:1427	Milk protein yield	1410:1427	Milk protein yield	1410:1427	Milk protein yield was greater for cows fed BMR (1.49 kg/d) and BMRFL (1.54 kg/d) compared with CONV (1.36 kg/d).
31447165	16	3	theme	N	2306:2306	arg1	efficiency					2308:2317	milk N efficiency	2301:2317	milk N efficiency	2301:2317	In addition, feeding the BMRFL corn silage improved efficiency of component-corrected milk production and milk N efficiency compared with the CONV and BMR silages.
31447165	8	4	theme	Milk	1301:1304	arg1	yield					1310:1314	Milk fat yield	1301:1314	Milk fat yield	1301:1314	Milk fat yield was higher for cows fed BMRFL (1.87 kg/d) compared with CONV (1.74 kg/d) and BMR (1.80 kg/d).
31447165	2	5	theme	total	538:542	arg1	ration					550:555	a total mixed ration	536:555	a total mixed ration	536:555	Cows were housed in tiestalls, milked 3 times/d, and fed a total mixed ration containing 49.0% (dry matter basis) of (1) a conventional corn silage hybrid (CONV); (2) a brown midrib bm3 hybrid (BMR); or (3) a bm3 hybrid with floury kernel genetics (BMRFL).
31447165	5	6	dep	Cary	991:994	arg1	NC					997:998	NC	997:998	NC	997:998	Data were analyzed by ANOVA using the MIXED procedure in SAS (SAS Institute Inc., Cary, NC).
31447165	2	7	theme	hybrid	627:632	arg1	%					572:572	49.0%	568:572	49.0% (dry matter basis) of (1) a conventional corn silage hybrid (CONV); (2) a brown midrib bm3 hybrid (BMR)	568:676	Cows were housed in tiestalls, milked 3 times/d, and fed a total mixed ration containing 49.0% (dry matter basis) of (1) a conventional corn silage hybrid (CONV); (2) a brown midrib bm3 hybrid (BMR); or (3) a bm3 hybrid with floury kernel genetics (BMRFL).
31447165	2	7	theme	hybrid	627:632	arg1	CONV					635:638	CONV	635:638	CONV	635:638	Cows were housed in tiestalls, milked 3 times/d, and fed a total mixed ration containing 49.0% (dry matter basis) of (1) a conventional corn silage hybrid (CONV); (2) a brown midrib bm3 hybrid (BMR); or (3) a bm3 hybrid with floury kernel genetics (BMRFL).
31447165	2	7	theme	hybrid	627:632	arg1	hybrid					627:632	a conventional corn silage hybrid	600:632	(1) a conventional corn silage hybrid (CONV); (2) a brown midrib bm3 hybrid (BMR)	596:676	Cows were housed in tiestalls, milked 3 times/d, and fed a total mixed ration containing 49.0% (dry matter basis) of (1) a conventional corn silage hybrid (CONV); (2) a brown midrib bm3 hybrid (BMR); or (3) a bm3 hybrid with floury kernel genetics (BMRFL).
31447165	2	7	theme	hybrid	627:632	arg1	basis					586:590	dry matter basis	575:590	dry matter basis	575:590	Cows were housed in tiestalls, milked 3 times/d, and fed a total mixed ration containing 49.0% (dry matter basis) of (1) a conventional corn silage hybrid (CONV); (2) a brown midrib bm3 hybrid (BMR); or (3) a bm3 hybrid with floury kernel genetics (BMRFL).
31447165	3	8	dep	%	759:759	arg1	silage					770:775	hay crop silage	761:775	6.3% hay crop silage	756:775	All diets contained 6.3% hay crop silage and 44.7% concentrate.
31447165	15	9	theme	lactational	2088:2098	arg1	performance					2100:2110	Overall lactational performance	2080:2110	Overall lactational performance	2080:2110	Overall lactational performance was enhanced for cows fed diets containing both BMR and BMRFL hybrids versus CONV.
31447165	0	10	theme	feed	103:106	arg1	efficiency					108:117	feed efficiency	103:117	feed efficiency	103:117	Influence of a novel bm3 corn silage hybrid with floury kernel genetics on lactational performance and feed efficiency of Holstein cows.
31447165	9	11	theme	Milk	1410:1413	arg1	yield					1423:1427	Milk protein yield	1410:1427	Milk protein yield	1410:1427	Milk protein yield was greater for cows fed BMR (1.49 kg/d) and BMRFL (1.54 kg/d) compared with CONV (1.36 kg/d).
31447165	4	12	dep	starch	883:888	arg1	%					891:891	%	891:891	% of dry matter	891:905	Dietary nutrient composition averaged 32.7% neutral detergent fiber (NDF) and 26.3 starch (% of dry matter).
31447165	4	12	dep	starch	883:888	arg1	matter					900:905	dry matter	896:905	dry matter	896:905	Dietary nutrient composition averaged 32.7% neutral detergent fiber (NDF) and 26.3 starch (% of dry matter).
31447165	15	13	theme	BMRFL	2168:2172	arg1	hybrids					2174:2180	BMR and BMRFL hybrids	2160:2180	BMR and BMRFL hybrids	2160:2180	Overall lactational performance was enhanced for cows fed diets containing both BMR and BMRFL hybrids versus CONV.
31447165	6	14	theme	matter	1010:1015	arg1	greater					1028:1034	greater	1028:1034	greater	1028:1034	The dry matter intake was greater for cows fed BMR (28.0 kg/d) compared with CONV (26.8 kg/d), whereas dry matter intake for cows fed BMRFL was intermediate (27.6 kg/d).
31447165	6	14	theme	matter	1010:1015	arg1	intake					1017:1022	The dry matter intake	1002:1022	The dry matter intake	1002:1022	The dry matter intake was greater for cows fed BMR (28.0 kg/d) compared with CONV (26.8 kg/d), whereas dry matter intake for cows fed BMRFL was intermediate (27.6 kg/d).
31447165	0	15	theme	cows	131:134	arg1	performance					87:97	lactational performance	75:97	lactational performance	75:97	Influence of a novel bm3 corn silage hybrid with floury kernel genetics on lactational performance and feed efficiency of Holstein cows.
31447165	0	15	theme	cows	131:134	arg1	efficiency					108:117	feed efficiency	103:117	feed efficiency	103:117	Influence of a novel bm3 corn silage hybrid with floury kernel genetics on lactational performance and feed efficiency of Holstein cows.
31447165	15	16	theme	BMR	2160:2162	arg1	hybrids					2174:2180	BMR and BMRFL hybrids	2160:2180	BMR and BMRFL hybrids	2160:2180	Overall lactational performance was enhanced for cows fed diets containing both BMR and BMRFL hybrids versus CONV.
31447165	14	17	theme	NDF	2049:2051	arg1	%					2058:2058	58.1%	2054:2058	58.1%	2054:2058	No differences were observed among diets in apparent total-tract digestibility of NDF (58.1%) or starch (99.3%).
31447165	14	17	theme	NDF	2049:2051	arg1	digestibility					2032:2044	apparent total-tract digestibility	2011:2044	apparent total-tract digestibility of NDF (58.1%)	2011:2059	No differences were observed among diets in apparent total-tract digestibility of NDF (58.1%) or starch (99.3%).
31447165	1	18	theme	available	376:384	arg1	hybrids					407:413	commercially available conventional and bm3 hybrids	363:413	commercially available conventional and bm3 hybrids	363:413	Dry matter intake, lactation performance, and chewing behavior of multiparous Holstein cows (n = 15) fed diets containing a novel bm3 corn silage hybrid with floury kernel genetics were compared with cows fed diets containing commercially available conventional and bm3 hybrids using a replicated 3 × 3 Latin square design with 28-d periods.
31447165	4	19	theme	matter	900:905	arg1	%					891:891	%	891:891	% of dry matter	891:905	Dietary nutrient composition averaged 32.7% neutral detergent fiber (NDF) and 26.3 starch (% of dry matter).
31447165	4	19	theme	matter	900:905	arg1	matter					900:905	dry matter	896:905	dry matter	896:905	Dietary nutrient composition averaged 32.7% neutral detergent fiber (NDF) and 26.3 starch (% of dry matter).
31447165	5	20	theme	MIXED	947:951	arg1	procedure					953:961	the MIXED procedure	943:961	the MIXED procedure in SAS (SAS Institute Inc., Cary, NC)	943:999	Data were analyzed by ANOVA using the MIXED procedure in SAS (SAS Institute Inc., Cary, NC).
31447165	1	21	theme	silage	276:281	arg1	hybrid					283:288	a novel bm3 corn silage hybrid	259:288	a novel bm3 corn silage hybrid with floury kernel genetics	259:316	Dry matter intake, lactation performance, and chewing behavior of multiparous Holstein cows (n = 15) fed diets containing a novel bm3 corn silage hybrid with floury kernel genetics were compared with cows fed diets containing commercially available conventional and bm3 hybrids using a replicated 3 × 3 Latin square design with 28-d periods.
31447165	0	22	theme	kernel	56:61	arg1	genetics					63:70	floury kernel genetics	49:70	floury kernel genetics	49:70	Influence of a novel bm3 corn silage hybrid with floury kernel genetics on lactational performance and feed efficiency of Holstein cows.
31447165	1	23	theme	matter	141:146	arg1	intake					148:153	Dry matter intake	137:153	Dry matter intake	137:153	Dry matter intake, lactation performance, and chewing behavior of multiparous Holstein cows (n = 15) fed diets containing a novel bm3 corn silage hybrid with floury kernel genetics were compared with cows fed diets containing commercially available conventional and bm3 hybrids using a replicated 3 × 3 Latin square design with 28-d periods.
31447165	11	24	theme	ECM/dry	1654:1660	arg1	intake					1669:1674	ECM/dry matter intake	1654:1674	ECM/dry matter intake	1654:1674	Feed efficiency (ECM/dry matter intake) was higher for cows fed BMRFL (1.87) compared with CONV (1.76) and BMR (1.79).
31447165	11	24	theme	ECM/dry	1654:1660	arg1	efficiency					1642:1651	Feed efficiency	1637:1651	Feed efficiency (ECM/dry matter intake)	1637:1675	Feed efficiency (ECM/dry matter intake) was higher for cows fed BMRFL (1.87) compared with CONV (1.76) and BMR (1.79).
31447165	0	25	from	Influence	0:8	arg1	performance					87:97	lactational performance	75:97	lactational performance	75:97	Influence of a novel bm3 corn silage hybrid with floury kernel genetics on lactational performance and feed efficiency of Holstein cows.
31447165	0	25	from	Influence	0:8	arg1	efficiency					108:117	feed efficiency	103:117	feed efficiency	103:117	Influence of a novel bm3 corn silage hybrid with floury kernel genetics on lactational performance and feed efficiency of Holstein cows.
31447165	16	26	theme	component-corrected	2261:2279	arg1	production					2286:2295	component-corrected milk production	2261:2295	component-corrected milk production	2261:2295	In addition, feeding the BMRFL corn silage improved efficiency of component-corrected milk production and milk N efficiency compared with the CONV and BMR silages.
31447165	1	27	theme	kernel	302:307	arg1	genetics					309:316	floury kernel genetics	295:316	floury kernel genetics	295:316	Dry matter intake, lactation performance, and chewing behavior of multiparous Holstein cows (n = 15) fed diets containing a novel bm3 corn silage hybrid with floury kernel genetics were compared with cows fed diets containing commercially available conventional and bm3 hybrids using a replicated 3 × 3 Latin square design with 28-d periods.
31447165	16	28	theme	production	2286:2295	arg1	efficiency					2247:2256	efficiency	2247:2256	efficiency of component-corrected milk production and milk N efficiency	2247:2317	In addition, feeding the BMRFL corn silage improved efficiency of component-corrected milk production and milk N efficiency compared with the CONV and BMR silages.
31447165	4	29	theme	Dietary	800:806	arg1	composition					817:827	Dietary nutrient composition	800:827	Dietary nutrient composition	800:827	Dietary nutrient composition averaged 32.7% neutral detergent fiber (NDF) and 26.3 starch (% of dry matter).
31447165	2	30	theme	kernel	711:716	arg1	BMRFL					728:732	BMRFL	728:732	BMRFL	728:732	Cows were housed in tiestalls, milked 3 times/d, and fed a total mixed ration containing 49.0% (dry matter basis) of (1) a conventional corn silage hybrid (CONV); (2) a brown midrib bm3 hybrid (BMR); or (3) a bm3 hybrid with floury kernel genetics (BMRFL).
31447165	2	30	theme	kernel	711:716	arg1	genetics					718:725	floury kernel genetics	704:725	floury kernel genetics (BMRFL)	704:733	Cows were housed in tiestalls, milked 3 times/d, and fed a total mixed ration containing 49.0% (dry matter basis) of (1) a conventional corn silage hybrid (CONV); (2) a brown midrib bm3 hybrid (BMR); or (3) a bm3 hybrid with floury kernel genetics (BMRFL).
31447165	2	31	theme	dry	575:577	arg1	%					572:572	49.0%	568:572	49.0% (dry matter basis) of (1) a conventional corn silage hybrid (CONV); (2) a brown midrib bm3 hybrid (BMR)	568:676	Cows were housed in tiestalls, milked 3 times/d, and fed a total mixed ration containing 49.0% (dry matter basis) of (1) a conventional corn silage hybrid (CONV); (2) a brown midrib bm3 hybrid (BMR); or (3) a bm3 hybrid with floury kernel genetics (BMRFL).
31447165	2	31	theme	dry	575:577	arg1	hybrid					627:632	a conventional corn silage hybrid	600:632	(1) a conventional corn silage hybrid (CONV); (2) a brown midrib bm3 hybrid (BMR)	596:676	Cows were housed in tiestalls, milked 3 times/d, and fed a total mixed ration containing 49.0% (dry matter basis) of (1) a conventional corn silage hybrid (CONV); (2) a brown midrib bm3 hybrid (BMR); or (3) a bm3 hybrid with floury kernel genetics (BMRFL).
31447165	2	31	theme	dry	575:577	arg1	basis					586:590	dry matter basis	575:590	dry matter basis	575:590	Cows were housed in tiestalls, milked 3 times/d, and fed a total mixed ration containing 49.0% (dry matter basis) of (1) a conventional corn silage hybrid (CONV); (2) a brown midrib bm3 hybrid (BMR); or (3) a bm3 hybrid with floury kernel genetics (BMRFL).
31447165	2	32	theme	corn	615:618	arg1	CONV					635:638	CONV	635:638	CONV	635:638	Cows were housed in tiestalls, milked 3 times/d, and fed a total mixed ration containing 49.0% (dry matter basis) of (1) a conventional corn silage hybrid (CONV); (2) a brown midrib bm3 hybrid (BMR); or (3) a bm3 hybrid with floury kernel genetics (BMRFL).
31447165	2	32	theme	corn	615:618	arg1	hybrid					627:632	a conventional corn silage hybrid	600:632	(1) a conventional corn silage hybrid (CONV); (2) a brown midrib bm3 hybrid (BMR)	596:676	Cows were housed in tiestalls, milked 3 times/d, and fed a total mixed ration containing 49.0% (dry matter basis) of (1) a conventional corn silage hybrid (CONV); (2) a brown midrib bm3 hybrid (BMR); or (3) a bm3 hybrid with floury kernel genetics (BMRFL).
31447165	7	33	theme	milk	1189:1192	arg1	yield					1200:1204	Energy-corrected milk (ECM) yield	1172:1204	Energy-corrected milk (ECM) yield	1172:1204	Energy-corrected milk (ECM) yield was greater for cows fed BMR (50.3 kg/d) and BMRFL (51.8 kg/d) compared with CONV (47.2 kg/d).
31447165	16	34	theme	milk	2301:2304	arg1	efficiency					2308:2317	milk N efficiency	2301:2317	milk N efficiency	2301:2317	In addition, feeding the BMRFL corn silage improved efficiency of component-corrected milk production and milk N efficiency compared with the CONV and BMR silages.
31447165	0	35	theme	bm3	21:23	arg1	hybrid					37:42	a novel bm3 corn silage hybrid	13:42	a novel bm3 corn silage hybrid with floury kernel genetics	13:70	Influence of a novel bm3 corn silage hybrid with floury kernel genetics on lactational performance and feed efficiency of Holstein cows.
31447165	12	36	theme	N	1761:1761	arg1	efficiency					1763:1772	Milk N efficiency	1756:1772	Milk N efficiency	1756:1772	Milk N efficiency was greatest for cows fed BMRFL (40.4%) followed by BMR (38.1%) and finally CONV (35.3%).
31447165	13	37	theme	NDF	1913:1915	arg1	kilograms					1900:1908	kilograms	1900:1908	kilograms of NDF consumed than cows fed either of the BMR hybrids	1900:1964	Cows fed CONV chewed 5 min more per kilograms of NDF consumed than cows fed either of the BMR hybrids.
31447165	0	38	theme	silage	30:35	arg1	hybrid					37:42	a novel bm3 corn silage hybrid	13:42	a novel bm3 corn silage hybrid with floury kernel genetics	13:70	Influence of a novel bm3 corn silage hybrid with floury kernel genetics on lactational performance and feed efficiency of Holstein cows.
31447165	1	39	theme	bm3	267:269	arg1	hybrid					283:288	a novel bm3 corn silage hybrid	259:288	a novel bm3 corn silage hybrid with floury kernel genetics	259:316	Dry matter intake, lactation performance, and chewing behavior of multiparous Holstein cows (n = 15) fed diets containing a novel bm3 corn silage hybrid with floury kernel genetics were compared with cows fed diets containing commercially available conventional and bm3 hybrids using a replicated 3 × 3 Latin square design with 28-d periods.
31447165	2	40	theme	bm3	661:663	arg1	BMR					673:675	BMR	673:675	BMR	673:675	Cows were housed in tiestalls, milked 3 times/d, and fed a total mixed ration containing 49.0% (dry matter basis) of (1) a conventional corn silage hybrid (CONV); (2) a brown midrib bm3 hybrid (BMR); or (3) a bm3 hybrid with floury kernel genetics (BMRFL).
31447165	2	40	theme	bm3	661:663	arg1	hybrid					665:670	a brown midrib bm3 hybrid	646:670	(1) a conventional corn silage hybrid (CONV); (2) a brown midrib bm3 hybrid (BMR)	596:676	Cows were housed in tiestalls, milked 3 times/d, and fed a total mixed ration containing 49.0% (dry matter basis) of (1) a conventional corn silage hybrid (CONV); (2) a brown midrib bm3 hybrid (BMR); or (3) a bm3 hybrid with floury kernel genetics (BMRFL).
31447165	16	41	theme	BMR	2346:2348	arg1	silages					2350:2356	the CONV and BMR silages	2333:2356	the CONV and BMR silages	2333:2356	In addition, feeding the BMRFL corn silage improved efficiency of component-corrected milk production and milk N efficiency compared with the CONV and BMR silages.
31447165	3	42	theme	crop	765:768	arg1	silage					770:775	hay crop silage	761:775	6.3% hay crop silage	756:775	All diets contained 6.3% hay crop silage and 44.7% concentrate.
31447165	2	43	theme	brown	648:652	arg1	BMR					673:675	BMR	673:675	BMR	673:675	Cows were housed in tiestalls, milked 3 times/d, and fed a total mixed ration containing 49.0% (dry matter basis) of (1) a conventional corn silage hybrid (CONV); (2) a brown midrib bm3 hybrid (BMR); or (3) a bm3 hybrid with floury kernel genetics (BMRFL).
31447165	2	43	theme	brown	648:652	arg1	hybrid					665:670	a brown midrib bm3 hybrid	646:670	(1) a conventional corn silage hybrid (CONV); (2) a brown midrib bm3 hybrid (BMR)	596:676	Cows were housed in tiestalls, milked 3 times/d, and fed a total mixed ration containing 49.0% (dry matter basis) of (1) a conventional corn silage hybrid (CONV); (2) a brown midrib bm3 hybrid (BMR); or (3) a bm3 hybrid with floury kernel genetics (BMRFL).
31447165	14	44	located	observed	1987:1994	arg1	%					2058:2058	58.1%	2054:2058	58.1%	2054:2058	No differences were observed among diets in apparent total-tract digestibility of NDF (58.1%) or starch (99.3%).
31447165	14	44	located	observed	1987:1994	arg1	%					2076:2076	99.3%	2072:2076	99.3%	2072:2076	No differences were observed among diets in apparent total-tract digestibility of NDF (58.1%) or starch (99.3%).
31447165	14	44	located	observed	1987:1994	arg1	starch					2064:2069	starch	2064:2069	starch	2064:2069	No differences were observed among diets in apparent total-tract digestibility of NDF (58.1%) or starch (99.3%).
31447165	14	44	located	observed	1987:1994	arg2	differences					1970:1980	No differences	1967:1980	No differences	1967:1980	No differences were observed among diets in apparent total-tract digestibility of NDF (58.1%) or starch (99.3%).
31447165	14	44	located	observed	1987:1994	arg1	digestibility					2032:2044	apparent total-tract digestibility	2011:2044	apparent total-tract digestibility of NDF (58.1%)	2011:2059	No differences were observed among diets in apparent total-tract digestibility of NDF (58.1%) or starch (99.3%).
31447165	1	45	theme	28-d	465:468	arg1	periods					470:476	28-d periods	465:476	28-d periods	465:476	Dry matter intake, lactation performance, and chewing behavior of multiparous Holstein cows (n = 15) fed diets containing a novel bm3 corn silage hybrid with floury kernel genetics were compared with cows fed diets containing commercially available conventional and bm3 hybrids using a replicated 3 × 3 Latin square design with 28-d periods.
31447165	1	46	theme	Holstein	215:222	arg1	n					230:230	n = 15	230:235	n = 15	230:235	Dry matter intake, lactation performance, and chewing behavior of multiparous Holstein cows (n = 15) fed diets containing a novel bm3 corn silage hybrid with floury kernel genetics were compared with cows fed diets containing commercially available conventional and bm3 hybrids using a replicated 3 × 3 Latin square design with 28-d periods.
31447165	1	46	theme	Holstein	215:222	arg1	cows					224:227	multiparous Holstein cows	203:227	multiparous Holstein cows (n = 15) fed diets containing a novel bm3 corn silage hybrid with floury kernel genetics	203:316	Dry matter intake, lactation performance, and chewing behavior of multiparous Holstein cows (n = 15) fed diets containing a novel bm3 corn silage hybrid with floury kernel genetics were compared with cows fed diets containing commercially available conventional and bm3 hybrids using a replicated 3 × 3 Latin square design with 28-d periods.
31447165	16	47	theme	corn	2226:2229	arg1	silage					2231:2236	the BMRFL corn silage	2216:2236	the BMRFL corn silage	2216:2236	In addition, feeding the BMRFL corn silage improved efficiency of component-corrected milk production and milk N efficiency compared with the CONV and BMR silages.
31447165	6	48	dep	intermediate	1146:1157	arg1	kg/d					1165:1168	27.6 kg/d	1160:1168	27.6 kg/d	1160:1168	The dry matter intake was greater for cows fed BMR (28.0 kg/d) compared with CONV (26.8 kg/d), whereas dry matter intake for cows fed BMRFL was intermediate (27.6 kg/d).
31447165	4	49	theme	neutral	844:850	arg1	fiber					862:866	neutral detergent fiber	844:866	32.7% neutral detergent fiber (NDF)	838:872	Dietary nutrient composition averaged 32.7% neutral detergent fiber (NDF) and 26.3 starch (% of dry matter).
31447165	4	49	theme	neutral	844:850	arg1	NDF					869:871	NDF	869:871	NDF	869:871	Dietary nutrient composition averaged 32.7% neutral detergent fiber (NDF) and 26.3 starch (% of dry matter).
31447165	2	50	theme	bm3	688:690	arg1	tiestalls					499:507	tiestalls	499:507	tiestalls	499:507	Cows were housed in tiestalls, milked 3 times/d, and fed a total mixed ration containing 49.0% (dry matter basis) of (1) a conventional corn silage hybrid (CONV); (2) a brown midrib bm3 hybrid (BMR); or (3) a bm3 hybrid with floury kernel genetics (BMRFL).
31447165	2	50	theme	bm3	688:690	arg1	hybrid					692:697	milked 3 times/d, and fed a total mixed ration containing 49.0% (dry matter basis) of (1) a conventional corn silage hybrid (CONV); (2) a brown midrib bm3 hybrid (BMR); or (3) a bm3 hybrid	510:697	milked 3 times/d, and fed a total mixed ration containing 49.0% (dry matter basis) of (1) a conventional corn silage hybrid (CONV); (2) a brown midrib bm3 hybrid (BMR); or (3) a bm3 hybrid with floury kernel genetics (BMRFL)	510:733	Cows were housed in tiestalls, milked 3 times/d, and fed a total mixed ration containing 49.0% (dry matter basis) of (1) a conventional corn silage hybrid (CONV); (2) a brown midrib bm3 hybrid (BMR); or (3) a bm3 hybrid with floury kernel genetics (BMRFL).
31447165	1	51	theme	bm3	403:405	arg1	hybrids					407:413	commercially available conventional and bm3 hybrids	363:413	commercially available conventional and bm3 hybrids	363:413	Dry matter intake, lactation performance, and chewing behavior of multiparous Holstein cows (n = 15) fed diets containing a novel bm3 corn silage hybrid with floury kernel genetics were compared with cows fed diets containing commercially available conventional and bm3 hybrids using a replicated 3 × 3 Latin square design with 28-d periods.
31447165	1	52	contain	containing	248:257	arg1	diets					242:246	diets	242:246	diets containing a novel bm3 corn silage hybrid with floury kernel genetics	242:316	Dry matter intake, lactation performance, and chewing behavior of multiparous Holstein cows (n = 15) fed diets containing a novel bm3 corn silage hybrid with floury kernel genetics were compared with cows fed diets containing commercially available conventional and bm3 hybrids using a replicated 3 × 3 Latin square design with 28-d periods.
31447165	1	52	contain	containing	248:257	arg2	hybrid					283:288	a novel bm3 corn silage hybrid	259:288	a novel bm3 corn silage hybrid with floury kernel genetics	259:316	Dry matter intake, lactation performance, and chewing behavior of multiparous Holstein cows (n = 15) fed diets containing a novel bm3 corn silage hybrid with floury kernel genetics were compared with cows fed diets containing commercially available conventional and bm3 hybrids using a replicated 3 × 3 Latin square design with 28-d periods.
31447165	6	53	theme	matter	1109:1114	arg1	intake					1116:1121	dry matter intake	1105:1121	dry matter intake for cows fed BMRFL	1105:1140	The dry matter intake was greater for cows fed BMR (28.0 kg/d) compared with CONV (26.8 kg/d), whereas dry matter intake for cows fed BMRFL was intermediate (27.6 kg/d).
31447165	1	54	theme	=	232:232	arg1	n					230:230	n = 15	230:235	n = 15	230:235	Dry matter intake, lactation performance, and chewing behavior of multiparous Holstein cows (n = 15) fed diets containing a novel bm3 corn silage hybrid with floury kernel genetics were compared with cows fed diets containing commercially available conventional and bm3 hybrids using a replicated 3 × 3 Latin square design with 28-d periods.
31447165	1	54	theme	=	232:232	arg1	cows					224:227	multiparous Holstein cows	203:227	multiparous Holstein cows (n = 15) fed diets containing a novel bm3 corn silage hybrid with floury kernel genetics	203:316	Dry matter intake, lactation performance, and chewing behavior of multiparous Holstein cows (n = 15) fed diets containing a novel bm3 corn silage hybrid with floury kernel genetics were compared with cows fed diets containing commercially available conventional and bm3 hybrids using a replicated 3 × 3 Latin square design with 28-d periods.
31447165	2	55	theme	times/d	519:525	arg1	tiestalls					499:507	tiestalls	499:507	tiestalls	499:507	Cows were housed in tiestalls, milked 3 times/d, and fed a total mixed ration containing 49.0% (dry matter basis) of (1) a conventional corn silage hybrid (CONV); (2) a brown midrib bm3 hybrid (BMR); or (3) a bm3 hybrid with floury kernel genetics (BMRFL).
31447165	2	55	theme	times/d	519:525	arg1	hybrid					692:697	milked 3 times/d, and fed a total mixed ration containing 49.0% (dry matter basis) of (1) a conventional corn silage hybrid (CONV); (2) a brown midrib bm3 hybrid (BMR); or (3) a bm3 hybrid	510:697	milked 3 times/d, and fed a total mixed ration containing 49.0% (dry matter basis) of (1) a conventional corn silage hybrid (CONV); (2) a brown midrib bm3 hybrid (BMR); or (3) a bm3 hybrid with floury kernel genetics (BMRFL)	510:733	Cows were housed in tiestalls, milked 3 times/d, and fed a total mixed ration containing 49.0% (dry matter basis) of (1) a conventional corn silage hybrid (CONV); (2) a brown midrib bm3 hybrid (BMR); or (3) a bm3 hybrid with floury kernel genetics (BMRFL).
31447165	13	56	theme	BMR	1954:1956	arg1	hybrids					1958:1964	the BMR hybrids	1950:1964	the BMR hybrids	1950:1964	Cows fed CONV chewed 5 min more per kilograms of NDF consumed than cows fed either of the BMR hybrids.
31447165	16	57	theme	BMRFL	2220:2224	arg1	silage					2231:2236	the BMRFL corn silage	2216:2236	the BMRFL corn silage	2216:2236	In addition, feeding the BMRFL corn silage improved efficiency of component-corrected milk production and milk N efficiency compared with the CONV and BMR silages.
31447165	2	58	theme	mixed	544:548	arg1	ration					550:555	a total mixed ration	536:555	a total mixed ration	536:555	Cows were housed in tiestalls, milked 3 times/d, and fed a total mixed ration containing 49.0% (dry matter basis) of (1) a conventional corn silage hybrid (CONV); (2) a brown midrib bm3 hybrid (BMR); or (3) a bm3 hybrid with floury kernel genetics (BMRFL).
31447165	4	59	theme	dry	896:898	arg1	matter					900:905	dry matter	896:905	dry matter	896:905	Dietary nutrient composition averaged 32.7% neutral detergent fiber (NDF) and 26.3 starch (% of dry matter).
31447165	1	60	theme	replicated	423:432	arg1	design					453:458	a replicated 3 × 3 Latin square design	421:458	a replicated 3 × 3 Latin square design	421:458	Dry matter intake, lactation performance, and chewing behavior of multiparous Holstein cows (n = 15) fed diets containing a novel bm3 corn silage hybrid with floury kernel genetics were compared with cows fed diets containing commercially available conventional and bm3 hybrids using a replicated 3 × 3 Latin square design with 28-d periods.
31447165	16	61	theme	efficiency	2308:2317	arg1	efficiency					2247:2256	efficiency	2247:2256	efficiency of component-corrected milk production and milk N efficiency	2247:2317	In addition, feeding the BMRFL corn silage improved efficiency of component-corrected milk production and milk N efficiency compared with the CONV and BMR silages.
31447165	1	62	theme	lactation	156:164	arg1	performance					166:176	lactation performance	156:176	lactation performance	156:176	Dry matter intake, lactation performance, and chewing behavior of multiparous Holstein cows (n = 15) fed diets containing a novel bm3 corn silage hybrid with floury kernel genetics were compared with cows fed diets containing commercially available conventional and bm3 hybrids using a replicated 3 × 3 Latin square design with 28-d periods.
31447165	1	63	theme	×	436:436	arg1	design					453:458	a replicated 3 × 3 Latin square design	421:458	a replicated 3 × 3 Latin square design	421:458	Dry matter intake, lactation performance, and chewing behavior of multiparous Holstein cows (n = 15) fed diets containing a novel bm3 corn silage hybrid with floury kernel genetics were compared with cows fed diets containing commercially available conventional and bm3 hybrids using a replicated 3 × 3 Latin square design with 28-d periods.
31447165	8	64	theme	fat	1306:1308	arg1	yield					1310:1314	Milk fat yield	1301:1314	Milk fat yield	1301:1314	Milk fat yield was higher for cows fed BMRFL (1.87 kg/d) compared with CONV (1.74 kg/d) and BMR (1.80 kg/d).
31447165	0	65	theme	Holstein	122:129	arg1	cows					131:134	Holstein cows	122:134	Holstein cows	122:134	Influence of a novel bm3 corn silage hybrid with floury kernel genetics on lactational performance and feed efficiency of Holstein cows.
31447165	16	66	theme	CONV	2337:2340	arg1	silages					2350:2356	the CONV and BMR silages	2333:2356	the CONV and BMR silages	2333:2356	In addition, feeding the BMRFL corn silage improved efficiency of component-corrected milk production and milk N efficiency compared with the CONV and BMR silages.
31447165	1	67	theme	chewing	183:189	arg1	behavior					191:198	chewing behavior	183:198	chewing behavior	183:198	Dry matter intake, lactation performance, and chewing behavior of multiparous Holstein cows (n = 15) fed diets containing a novel bm3 corn silage hybrid with floury kernel genetics were compared with cows fed diets containing commercially available conventional and bm3 hybrids using a replicated 3 × 3 Latin square design with 28-d periods.
31447165	2	68	dep	times/d	519:525	arg1	containing					557:566	containing	557:566	containing 49.0% (dry matter basis) of (1) a conventional corn silage hybrid (CONV); (2) a brown midrib bm3 hybrid (BMR)	557:676	Cows were housed in tiestalls, milked 3 times/d, and fed a total mixed ration containing 49.0% (dry matter basis) of (1) a conventional corn silage hybrid (CONV); (2) a brown midrib bm3 hybrid (BMR); or (3) a bm3 hybrid with floury kernel genetics (BMRFL).
31447165	2	68	dep	times/d	519:525	arg1	3					683:683	3	683:683	3	683:683	Cows were housed in tiestalls, milked 3 times/d, and fed a total mixed ration containing 49.0% (dry matter basis) of (1) a conventional corn silage hybrid (CONV); (2) a brown midrib bm3 hybrid (BMR); or (3) a bm3 hybrid with floury kernel genetics (BMRFL).
31447165	16	69	theme	milk	2281:2284	arg1	production					2286:2295	component-corrected milk production	2261:2295	component-corrected milk production	2261:2295	In addition, feeding the BMRFL corn silage improved efficiency of component-corrected milk production and milk N efficiency compared with the CONV and BMR silages.
31447165	2	70	theme	milked	510:515	arg1	tiestalls					499:507	tiestalls	499:507	tiestalls	499:507	Cows were housed in tiestalls, milked 3 times/d, and fed a total mixed ration containing 49.0% (dry matter basis) of (1) a conventional corn silage hybrid (CONV); (2) a brown midrib bm3 hybrid (BMR); or (3) a bm3 hybrid with floury kernel genetics (BMRFL).
31447165	2	70	theme	milked	510:515	arg1	hybrid					692:697	milked 3 times/d, and fed a total mixed ration containing 49.0% (dry matter basis) of (1) a conventional corn silage hybrid (CONV); (2) a brown midrib bm3 hybrid (BMR); or (3) a bm3 hybrid	510:697	milked 3 times/d, and fed a total mixed ration containing 49.0% (dry matter basis) of (1) a conventional corn silage hybrid (CONV); (2) a brown midrib bm3 hybrid (BMR); or (3) a bm3 hybrid with floury kernel genetics (BMRFL)	510:733	Cows were housed in tiestalls, milked 3 times/d, and fed a total mixed ration containing 49.0% (dry matter basis) of (1) a conventional corn silage hybrid (CONV); (2) a brown midrib bm3 hybrid (BMR); or (3) a bm3 hybrid with floury kernel genetics (BMRFL).
31447165	6	71	theme	dry	1006:1008	arg1	greater					1028:1034	greater	1028:1034	greater	1028:1034	The dry matter intake was greater for cows fed BMR (28.0 kg/d) compared with CONV (26.8 kg/d), whereas dry matter intake for cows fed BMRFL was intermediate (27.6 kg/d).
31447165	6	71	theme	dry	1006:1008	arg1	intake					1017:1022	The dry matter intake	1002:1022	The dry matter intake	1002:1022	The dry matter intake was greater for cows fed BMR (28.0 kg/d) compared with CONV (26.8 kg/d), whereas dry matter intake for cows fed BMRFL was intermediate (27.6 kg/d).
31447165	2	72	theme	silage	620:625	arg1	CONV					635:638	CONV	635:638	CONV	635:638	Cows were housed in tiestalls, milked 3 times/d, and fed a total mixed ration containing 49.0% (dry matter basis) of (1) a conventional corn silage hybrid (CONV); (2) a brown midrib bm3 hybrid (BMR); or (3) a bm3 hybrid with floury kernel genetics (BMRFL).
31447165	2	72	theme	silage	620:625	arg1	hybrid					627:632	a conventional corn silage hybrid	600:632	(1) a conventional corn silage hybrid (CONV); (2) a brown midrib bm3 hybrid (BMR)	596:676	Cows were housed in tiestalls, milked 3 times/d, and fed a total mixed ration containing 49.0% (dry matter basis) of (1) a conventional corn silage hybrid (CONV); (2) a brown midrib bm3 hybrid (BMR); or (3) a bm3 hybrid with floury kernel genetics (BMRFL).
31447165	0	73	theme	floury	49:54	arg1	genetics					63:70	floury kernel genetics	49:70	floury kernel genetics	49:70	Influence of a novel bm3 corn silage hybrid with floury kernel genetics on lactational performance and feed efficiency of Holstein cows.
31447165	1	74	theme	conventional	386:397	arg1	hybrids					407:413	commercially available conventional and bm3 hybrids	363:413	commercially available conventional and bm3 hybrids	363:413	Dry matter intake, lactation performance, and chewing behavior of multiparous Holstein cows (n = 15) fed diets containing a novel bm3 corn silage hybrid with floury kernel genetics were compared with cows fed diets containing commercially available conventional and bm3 hybrids using a replicated 3 × 3 Latin square design with 28-d periods.
31447165	4	75	theme	detergent	852:860	arg1	fiber					862:866	neutral detergent fiber	844:866	32.7% neutral detergent fiber (NDF)	838:872	Dietary nutrient composition averaged 32.7% neutral detergent fiber (NDF) and 26.3 starch (% of dry matter).
31447165	4	75	theme	detergent	852:860	arg1	NDF					869:871	NDF	869:871	NDF	869:871	Dietary nutrient composition averaged 32.7% neutral detergent fiber (NDF) and 26.3 starch (% of dry matter).
31447165	11	76	theme	Feed	1637:1640	arg1	intake					1669:1674	ECM/dry matter intake	1654:1674	ECM/dry matter intake	1654:1674	Feed efficiency (ECM/dry matter intake) was higher for cows fed BMRFL (1.87) compared with CONV (1.76) and BMR (1.79).
31447165	11	76	theme	Feed	1637:1640	arg1	efficiency					1642:1651	Feed efficiency	1637:1651	Feed efficiency (ECM/dry matter intake)	1637:1675	Feed efficiency (ECM/dry matter intake) was higher for cows fed BMRFL (1.87) compared with CONV (1.76) and BMR (1.79).
31447165	14	77	theme	total-tract	2020:2030	arg1	%					2058:2058	58.1%	2054:2058	58.1%	2054:2058	No differences were observed among diets in apparent total-tract digestibility of NDF (58.1%) or starch (99.3%).
31447165	14	77	theme	total-tract	2020:2030	arg1	digestibility					2032:2044	apparent total-tract digestibility	2011:2044	apparent total-tract digestibility of NDF (58.1%)	2011:2059	No differences were observed among diets in apparent total-tract digestibility of NDF (58.1%) or starch (99.3%).
31447165	2	78	theme	conventional	602:613	arg1	CONV					635:638	CONV	635:638	CONV	635:638	Cows were housed in tiestalls, milked 3 times/d, and fed a total mixed ration containing 49.0% (dry matter basis) of (1) a conventional corn silage hybrid (CONV); (2) a brown midrib bm3 hybrid (BMR); or (3) a bm3 hybrid with floury kernel genetics (BMRFL).
31447165	2	78	theme	conventional	602:613	arg1	hybrid					627:632	a conventional corn silage hybrid	600:632	(1) a conventional corn silage hybrid (CONV); (2) a brown midrib bm3 hybrid (BMR)	596:676	Cows were housed in tiestalls, milked 3 times/d, and fed a total mixed ration containing 49.0% (dry matter basis) of (1) a conventional corn silage hybrid (CONV); (2) a brown midrib bm3 hybrid (BMR); or (3) a bm3 hybrid with floury kernel genetics (BMRFL).
31447165	1	79	theme	Dry	137:139	arg1	intake					148:153	Dry matter intake	137:153	Dry matter intake	137:153	Dry matter intake, lactation performance, and chewing behavior of multiparous Holstein cows (n = 15) fed diets containing a novel bm3 corn silage hybrid with floury kernel genetics were compared with cows fed diets containing commercially available conventional and bm3 hybrids using a replicated 3 × 3 Latin square design with 28-d periods.
31447165	2	80	dep	containing	557:566	arg1	fed					532:534	fed	532:534	fed	532:534	Cows were housed in tiestalls, milked 3 times/d, and fed a total mixed ration containing 49.0% (dry matter basis) of (1) a conventional corn silage hybrid (CONV); (2) a brown midrib bm3 hybrid (BMR); or (3) a bm3 hybrid with floury kernel genetics (BMRFL).
31447165	5	81	from	procedure	953:961	arg1	SAS					966:968	SAS	966:968	SAS (SAS Institute Inc., Cary, NC)	966:999	Data were analyzed by ANOVA using the MIXED procedure in SAS (SAS Institute Inc., Cary, NC).
31447165	5	81	from	procedure	953:961	arg1	Cary					991:994	Cary	991:994	Cary	991:994	Data were analyzed by ANOVA using the MIXED procedure in SAS (SAS Institute Inc., Cary, NC).
31447165	0	82	theme	lactational	75:85	arg1	performance					87:97	lactational performance	75:97	lactational performance	75:97	Influence of a novel bm3 corn silage hybrid with floury kernel genetics on lactational performance and feed efficiency of Holstein cows.
31447165	1	83	theme	floury	295:300	arg1	genetics					309:316	floury kernel genetics	295:316	floury kernel genetics	295:316	Dry matter intake, lactation performance, and chewing behavior of multiparous Holstein cows (n = 15) fed diets containing a novel bm3 corn silage hybrid with floury kernel genetics were compared with cows fed diets containing commercially available conventional and bm3 hybrids using a replicated 3 × 3 Latin square design with 28-d periods.
31447165	4	84	dep	%	842:842	arg1	fiber					862:866	neutral detergent fiber	844:866	32.7% neutral detergent fiber (NDF)	838:872	Dietary nutrient composition averaged 32.7% neutral detergent fiber (NDF) and 26.3 starch (% of dry matter).
31447165	4	84	dep	%	842:842	arg1	NDF					869:871	NDF	869:871	NDF	869:871	Dietary nutrient composition averaged 32.7% neutral detergent fiber (NDF) and 26.3 starch (% of dry matter).
31447165	1	85	with	hybrid	283:288	arg1	genetics					309:316	floury kernel genetics	295:316	floury kernel genetics	295:316	Dry matter intake, lactation performance, and chewing behavior of multiparous Holstein cows (n = 15) fed diets containing a novel bm3 corn silage hybrid with floury kernel genetics were compared with cows fed diets containing commercially available conventional and bm3 hybrids using a replicated 3 × 3 Latin square design with 28-d periods.
31447165	7	86	theme	ECM	1195:1197	arg1	yield					1200:1204	Energy-corrected milk (ECM) yield	1172:1204	Energy-corrected milk (ECM) yield	1172:1204	Energy-corrected milk (ECM) yield was greater for cows fed BMR (50.3 kg/d) and BMRFL (51.8 kg/d) compared with CONV (47.2 kg/d).
31447165	11	87	theme	matter	1662:1667	arg1	intake					1669:1674	ECM/dry matter intake	1654:1674	ECM/dry matter intake	1654:1674	Feed efficiency (ECM/dry matter intake) was higher for cows fed BMRFL (1.87) compared with CONV (1.76) and BMR (1.79).
31447165	11	87	theme	matter	1662:1667	arg1	efficiency					1642:1651	Feed efficiency	1637:1651	Feed efficiency (ECM/dry matter intake)	1637:1675	Feed efficiency (ECM/dry matter intake) was higher for cows fed BMRFL (1.87) compared with CONV (1.76) and BMR (1.79).
31447165	15	88	theme	Overall	2080:2086	arg1	performance					2100:2110	Overall lactational performance	2080:2110	Overall lactational performance	2080:2110	Overall lactational performance was enhanced for cows fed diets containing both BMR and BMRFL hybrids versus CONV.
31447165	10	89	theme	Milk	1524:1527	arg1	urea-N					1529:1534	Milk urea-N	1524:1534	Milk urea-N	1524:1534	Milk urea-N was reduced for cows fed BMR (11.61 mg/dL) and BMRFL (11.16 mg/dL) compared with CONV (13.60 mg/dL).
31447165	14	90	theme	apparent	2011:2018	arg1	%					2058:2058	58.1%	2054:2058	58.1%	2054:2058	No differences were observed among diets in apparent total-tract digestibility of NDF (58.1%) or starch (99.3%).
31447165	14	90	theme	apparent	2011:2018	arg1	digestibility					2032:2044	apparent total-tract digestibility	2011:2044	apparent total-tract digestibility of NDF (58.1%)	2011:2059	No differences were observed among diets in apparent total-tract digestibility of NDF (58.1%) or starch (99.3%).
31447165	3	91	contain	contained	746:754	arg2	%					785:785	44.7%	781:785	44.7% concentrate	781:797	All diets contained 6.3% hay crop silage and 44.7% concentrate.
31447165	3	91	contain	contained	746:754	arg1	diets					740:744	All diets	736:744	All diets	736:744	All diets contained 6.3% hay crop silage and 44.7% concentrate.
31447165	3	91	contain	contained	746:754	arg2	%					759:759	6.3%	756:759	6.3% hay crop silage	756:775	All diets contained 6.3% hay crop silage and 44.7% concentrate.
31447165	15	92	contain	containing	2144:2153	arg2	CONV					2189:2192	CONV	2189:2192	CONV	2189:2192	Overall lactational performance was enhanced for cows fed diets containing both BMR and BMRFL hybrids versus CONV.
31447165	15	92	contain	containing	2144:2153	arg2	hybrids					2174:2180	BMR and BMRFL hybrids	2160:2180	BMR and BMRFL hybrids	2160:2180	Overall lactational performance was enhanced for cows fed diets containing both BMR and BMRFL hybrids versus CONV.
31447165	15	92	contain	containing	2144:2153	arg1	diets					2138:2142	diets	2138:2142	diets containing both BMR and BMRFL hybrids versus CONV	2138:2192	Overall lactational performance was enhanced for cows fed diets containing both BMR and BMRFL hybrids versus CONV.
31447165	6	93	theme	dry	1105:1107	arg1	intake					1116:1121	dry matter intake	1105:1121	dry matter intake for cows fed BMRFL	1105:1140	The dry matter intake was greater for cows fed BMR (28.0 kg/d) compared with CONV (26.8 kg/d), whereas dry matter intake for cows fed BMRFL was intermediate (27.6 kg/d).
31447165	2	94	theme	matter	579:584	arg1	%					572:572	49.0%	568:572	49.0% (dry matter basis) of (1) a conventional corn silage hybrid (CONV); (2) a brown midrib bm3 hybrid (BMR)	568:676	Cows were housed in tiestalls, milked 3 times/d, and fed a total mixed ration containing 49.0% (dry matter basis) of (1) a conventional corn silage hybrid (CONV); (2) a brown midrib bm3 hybrid (BMR); or (3) a bm3 hybrid with floury kernel genetics (BMRFL).
31447165	2	94	theme	matter	579:584	arg1	hybrid					627:632	a conventional corn silage hybrid	600:632	(1) a conventional corn silage hybrid (CONV); (2) a brown midrib bm3 hybrid (BMR)	596:676	Cows were housed in tiestalls, milked 3 times/d, and fed a total mixed ration containing 49.0% (dry matter basis) of (1) a conventional corn silage hybrid (CONV); (2) a brown midrib bm3 hybrid (BMR); or (3) a bm3 hybrid with floury kernel genetics (BMRFL).
31447165	2	94	theme	matter	579:584	arg1	basis					586:590	dry matter basis	575:590	dry matter basis	575:590	Cows were housed in tiestalls, milked 3 times/d, and fed a total mixed ration containing 49.0% (dry matter basis) of (1) a conventional corn silage hybrid (CONV); (2) a brown midrib bm3 hybrid (BMR); or (3) a bm3 hybrid with floury kernel genetics (BMRFL).
31447165	0	95	theme	novel	15:19	arg1	hybrid					37:42	a novel bm3 corn silage hybrid	13:42	a novel bm3 corn silage hybrid with floury kernel genetics	13:70	Influence of a novel bm3 corn silage hybrid with floury kernel genetics on lactational performance and feed efficiency of Holstein cows.
31447165	2	96	with	hybrid	692:697	arg1	BMRFL					728:732	BMRFL	728:732	BMRFL	728:732	Cows were housed in tiestalls, milked 3 times/d, and fed a total mixed ration containing 49.0% (dry matter basis) of (1) a conventional corn silage hybrid (CONV); (2) a brown midrib bm3 hybrid (BMR); or (3) a bm3 hybrid with floury kernel genetics (BMRFL).
31447165	2	96	with	hybrid	692:697	arg1	genetics					718:725	floury kernel genetics	704:725	floury kernel genetics (BMRFL)	704:733	Cows were housed in tiestalls, milked 3 times/d, and fed a total mixed ration containing 49.0% (dry matter basis) of (1) a conventional corn silage hybrid (CONV); (2) a brown midrib bm3 hybrid (BMR); or (3) a bm3 hybrid with floury kernel genetics (BMRFL).
31447165	4	97	theme	nutrient	808:815	arg1	composition					817:827	Dietary nutrient composition	800:827	Dietary nutrient composition	800:827	Dietary nutrient composition averaged 32.7% neutral detergent fiber (NDF) and 26.3 starch (% of dry matter).
31447165	2	98	theme	floury	704:709	arg1	BMRFL					728:732	BMRFL	728:732	BMRFL	728:732	Cows were housed in tiestalls, milked 3 times/d, and fed a total mixed ration containing 49.0% (dry matter basis) of (1) a conventional corn silage hybrid (CONV); (2) a brown midrib bm3 hybrid (BMR); or (3) a bm3 hybrid with floury kernel genetics (BMRFL).
31447165	2	98	theme	floury	704:709	arg1	genetics					718:725	floury kernel genetics	704:725	floury kernel genetics (BMRFL)	704:733	Cows were housed in tiestalls, milked 3 times/d, and fed a total mixed ration containing 49.0% (dry matter basis) of (1) a conventional corn silage hybrid (CONV); (2) a brown midrib bm3 hybrid (BMR); or (3) a bm3 hybrid with floury kernel genetics (BMRFL).
31447165	0	99	theme	corn	25:28	arg1	hybrid					37:42	a novel bm3 corn silage hybrid	13:42	a novel bm3 corn silage hybrid with floury kernel genetics	13:70	Influence of a novel bm3 corn silage hybrid with floury kernel genetics on lactational performance and feed efficiency of Holstein cows.
31447165	1	100	contain	containing	352:361	arg1	diets					346:350	diets	346:350	diets containing commercially available conventional and bm3 hybrids	346:413	Dry matter intake, lactation performance, and chewing behavior of multiparous Holstein cows (n = 15) fed diets containing a novel bm3 corn silage hybrid with floury kernel genetics were compared with cows fed diets containing commercially available conventional and bm3 hybrids using a replicated 3 × 3 Latin square design with 28-d periods.
31447165	1	100	contain	containing	352:361	arg2	hybrids					407:413	commercially available conventional and bm3 hybrids	363:413	commercially available conventional and bm3 hybrids	363:413	Dry matter intake, lactation performance, and chewing behavior of multiparous Holstein cows (n = 15) fed diets containing a novel bm3 corn silage hybrid with floury kernel genetics were compared with cows fed diets containing commercially available conventional and bm3 hybrids using a replicated 3 × 3 Latin square design with 28-d periods.
31447165	0	101	theme	hybrid	37:42	arg1	Influence					0:8	Influence	0:8	Influence of a novel bm3 corn silage hybrid with floury kernel genetics on lactational performance and feed efficiency of Holstein cows.	0:135	Influence of a novel bm3 corn silage hybrid with floury kernel genetics on lactational performance and feed efficiency of Holstein cows.
31447165	1	102	theme	novel	261:265	arg1	hybrid					283:288	a novel bm3 corn silage hybrid	259:288	a novel bm3 corn silage hybrid with floury kernel genetics	259:316	Dry matter intake, lactation performance, and chewing behavior of multiparous Holstein cows (n = 15) fed diets containing a novel bm3 corn silage hybrid with floury kernel genetics were compared with cows fed diets containing commercially available conventional and bm3 hybrids using a replicated 3 × 3 Latin square design with 28-d periods.
31447165	7	103	theme	Energy-corrected	1172:1187	arg1	yield					1200:1204	Energy-corrected milk (ECM) yield	1172:1204	Energy-corrected milk (ECM) yield	1172:1204	Energy-corrected milk (ECM) yield was greater for cows fed BMR (50.3 kg/d) and BMRFL (51.8 kg/d) compared with CONV (47.2 kg/d).
31447165	1	104	theme	square	446:451	arg1	design					453:458	a replicated 3 × 3 Latin square design	421:458	a replicated 3 × 3 Latin square design	421:458	Dry matter intake, lactation performance, and chewing behavior of multiparous Holstein cows (n = 15) fed diets containing a novel bm3 corn silage hybrid with floury kernel genetics were compared with cows fed diets containing commercially available conventional and bm3 hybrids using a replicated 3 × 3 Latin square design with 28-d periods.
31447165	1	105	theme	corn	271:274	arg1	hybrid					283:288	a novel bm3 corn silage hybrid	259:288	a novel bm3 corn silage hybrid with floury kernel genetics	259:316	Dry matter intake, lactation performance, and chewing behavior of multiparous Holstein cows (n = 15) fed diets containing a novel bm3 corn silage hybrid with floury kernel genetics were compared with cows fed diets containing commercially available conventional and bm3 hybrids using a replicated 3 × 3 Latin square design with 28-d periods.
31447165	2	106	theme	midrib	654:659	arg1	BMR					673:675	BMR	673:675	BMR	673:675	Cows were housed in tiestalls, milked 3 times/d, and fed a total mixed ration containing 49.0% (dry matter basis) of (1) a conventional corn silage hybrid (CONV); (2) a brown midrib bm3 hybrid (BMR); or (3) a bm3 hybrid with floury kernel genetics (BMRFL).
31447165	2	106	theme	midrib	654:659	arg1	hybrid					665:670	a brown midrib bm3 hybrid	646:670	(1) a conventional corn silage hybrid (CONV); (2) a brown midrib bm3 hybrid (BMR)	596:676	Cows were housed in tiestalls, milked 3 times/d, and fed a total mixed ration containing 49.0% (dry matter basis) of (1) a conventional corn silage hybrid (CONV); (2) a brown midrib bm3 hybrid (BMR); or (3) a bm3 hybrid with floury kernel genetics (BMRFL).
31447165	0	107	with	hybrid	37:42	arg1	genetics					63:70	floury kernel genetics	49:70	floury kernel genetics	49:70	Influence of a novel bm3 corn silage hybrid with floury kernel genetics on lactational performance and feed efficiency of Holstein cows.
31447165	3	108	dep	%	785:785	arg1	concentrate					787:797	concentrate	787:797	44.7% concentrate	781:797	All diets contained 6.3% hay crop silage and 44.7% concentrate.
31447165	1	109	theme	multiparous	203:213	arg1	n					230:230	n = 15	230:235	n = 15	230:235	Dry matter intake, lactation performance, and chewing behavior of multiparous Holstein cows (n = 15) fed diets containing a novel bm3 corn silage hybrid with floury kernel genetics were compared with cows fed diets containing commercially available conventional and bm3 hybrids using a replicated 3 × 3 Latin square design with 28-d periods.
31447165	1	109	theme	multiparous	203:213	arg1	cows					224:227	multiparous Holstein cows	203:227	multiparous Holstein cows (n = 15) fed diets containing a novel bm3 corn silage hybrid with floury kernel genetics	203:316	Dry matter intake, lactation performance, and chewing behavior of multiparous Holstein cows (n = 15) fed diets containing a novel bm3 corn silage hybrid with floury kernel genetics were compared with cows fed diets containing commercially available conventional and bm3 hybrids using a replicated 3 × 3 Latin square design with 28-d periods.
31447165	2	110	dep	hybrid	627:632	arg1	1					597:597	1	597:597	1	597:597	Cows were housed in tiestalls, milked 3 times/d, and fed a total mixed ration containing 49.0% (dry matter basis) of (1) a conventional corn silage hybrid (CONV); (2) a brown midrib bm3 hybrid (BMR); or (3) a bm3 hybrid with floury kernel genetics (BMRFL).
31447165	2	110	dep	hybrid	627:632	arg1	BMR					673:675	BMR	673:675	BMR	673:675	Cows were housed in tiestalls, milked 3 times/d, and fed a total mixed ration containing 49.0% (dry matter basis) of (1) a conventional corn silage hybrid (CONV); (2) a brown midrib bm3 hybrid (BMR); or (3) a bm3 hybrid with floury kernel genetics (BMRFL).
31447165	2	110	dep	hybrid	627:632	arg1	hybrid					665:670	a brown midrib bm3 hybrid	646:670	(1) a conventional corn silage hybrid (CONV); (2) a brown midrib bm3 hybrid (BMR)	596:676	Cows were housed in tiestalls, milked 3 times/d, and fed a total mixed ration containing 49.0% (dry matter basis) of (1) a conventional corn silage hybrid (CONV); (2) a brown midrib bm3 hybrid (BMR); or (3) a bm3 hybrid with floury kernel genetics (BMRFL).
31447165	12	111	theme	Milk	1756:1759	arg1	efficiency					1763:1772	Milk N efficiency	1756:1772	Milk N efficiency	1756:1772	Milk N efficiency was greatest for cows fed BMRFL (40.4%) followed by BMR (38.1%) and finally CONV (35.3%).
31447165	1	112	theme	cows	224:227	arg1	intake					148:153	Dry matter intake	137:153	Dry matter intake	137:153	Dry matter intake, lactation performance, and chewing behavior of multiparous Holstein cows (n = 15) fed diets containing a novel bm3 corn silage hybrid with floury kernel genetics were compared with cows fed diets containing commercially available conventional and bm3 hybrids using a replicated 3 × 3 Latin square design with 28-d periods.
31447165	1	112	theme	cows	224:227	arg1	performance					166:176	lactation performance	156:176	lactation performance	156:176	Dry matter intake, lactation performance, and chewing behavior of multiparous Holstein cows (n = 15) fed diets containing a novel bm3 corn silage hybrid with floury kernel genetics were compared with cows fed diets containing commercially available conventional and bm3 hybrids using a replicated 3 × 3 Latin square design with 28-d periods.
31447165	1	112	theme	cows	224:227	arg1	behavior					191:198	chewing behavior	183:198	chewing behavior	183:198	Dry matter intake, lactation performance, and chewing behavior of multiparous Holstein cows (n = 15) fed diets containing a novel bm3 corn silage hybrid with floury kernel genetics were compared with cows fed diets containing commercially available conventional and bm3 hybrids using a replicated 3 × 3 Latin square design with 28-d periods.
30594632	10	0	from	induction	1745:1753	arg1	AA					1820:1821	AA	1820:1821	AA	1820:1821	Among the various nanofilm combinations, the induction of osteogenic gene transcription in collagen (Col) and alginate (AA) film-coated MSCs was the most pronounced compared to that on other nanofilms.
30594632	10	0	from	induction	1745:1753	arg1	Col					1801:1803	Col	1801:1803	Col	1801:1803	Among the various nanofilm combinations, the induction of osteogenic gene transcription in collagen (Col) and alginate (AA) film-coated MSCs was the most pronounced compared to that on other nanofilms.
30594632	10	0	from	induction	1745:1753	arg1	alginate					1810:1817	alginate	1810:1817	alginate (AA)	1810:1822	Among the various nanofilm combinations, the induction of osteogenic gene transcription in collagen (Col) and alginate (AA) film-coated MSCs was the most pronounced compared to that on other nanofilms.
30594632	10	0	from	induction	1745:1753	arg1	collagen					1791:1798	collagen	1791:1798	collagen (Col)	1791:1804	Among the various nanofilm combinations, the induction of osteogenic gene transcription in collagen (Col) and alginate (AA) film-coated MSCs was the most pronounced compared to that on other nanofilms.
30594632	6	1	theme	extracellular	1167:1179	arg1	kinase					1196:1201	extracellular signal-related kinase	1167:1201	extracellular signal-related kinase	1167:1201	Further analysis revealed that the Col/AA nanofilm coating stimulated osteogenesis by activating transcriptional coactivators with the PDZ binding motif through extracellular signal-related kinase and p38 MAPK signaling.
30594632	13	2	from	applicable	2234:2243	arg1	bioengineering					2248:2261	bioengineering	2248:2261	bioengineering	2248:2261	Our results may be useful for developing a cell coating model system widely applicable in bioengineering and regenerative medicine.
30594632	13	2	from	applicable	2234:2243	arg1	medicine					2280:2287	regenerative medicine	2267:2287	regenerative medicine	2267:2287	Our results may be useful for developing a cell coating model system widely applicable in bioengineering and regenerative medicine.
30594632	11	3	theme	osteogenic	1999:2008	arg1	differentiation					2010:2024	MSC osteogenic differentiation	1995:2024	MSC osteogenic differentiation	1995:2024	A minimum number of Col/AA nanofilm bilayers (n = 2) was required for effective induction of MSC osteogenic differentiation.
30594632	10	4	dep	collagen	1791:1798	arg1	MSCs					1836:1839	film-coated MSCs	1824:1839	film-coated MSCs	1824:1839	Among the various nanofilm combinations, the induction of osteogenic gene transcription in collagen (Col) and alginate (AA) film-coated MSCs was the most pronounced compared to that on other nanofilms.
30594632	11	5	theme	MSC	1995:1997	arg1	differentiation					2010:2024	MSC osteogenic differentiation	1995:2024	MSC osteogenic differentiation	1995:2024	A minimum number of Col/AA nanofilm bilayers (n = 2) was required for effective induction of MSC osteogenic differentiation.
30594632	5	6	theme	osteogenic	928:937	arg1	differentiation					939:953	osteogenic differentiation	928:953	increased osteogenic differentiation efficiency	918:964	Interestingly, nanofilm composed of collagen (Col) and alginate (AA) showed relatively high stiffness and MSCs coated with the Col/AA nanofilm showed increased osteogenic differentiation efficiency compared to other nanofilm-coated MSCs.
30594632	4	7	theme	cell	698:701	arg1	surface					703:709	the cell surface	694:709	the cell surface	694:709	Here, we evaluated the osteogenic effects of a nanofilm in which oppositely charged polyelectrolytes were alternately adsorbed onto the cell surface to create an artificial ECM environment for single MSCs.
30594632	10	8	theme	nanofilm	1718:1725	arg1	combinations					1727:1738	the various nanofilm combinations	1706:1738	the various nanofilm combinations	1706:1738	Among the various nanofilm combinations, the induction of osteogenic gene transcription in collagen (Col) and alginate (AA) film-coated MSCs was the most pronounced compared to that on other nanofilms.
30594632	12	9	theme	differentiation	2111:2125	arg1	effect					2090:2095	the promoting effect	2076:2095	the promoting effect of osteogenic differentiation and stiffness of the nanofilm	2076:2155	In addition, we observed the correlation between the promoting effect of osteogenic differentiation and stiffness of the nanofilm.
30594632	10	10	theme	osteogenic	1758:1767	arg1	transcription					1774:1786	osteogenic gene transcription	1758:1786	osteogenic gene transcription	1758:1786	Among the various nanofilm combinations, the induction of osteogenic gene transcription in collagen (Col) and alginate (AA) film-coated MSCs was the most pronounced compared to that on other nanofilms.
30594632	11	11	theme	bilayers	1938:1945	arg1	number					1912:1917	A minimum number	1902:1917	A minimum number of Col/AA nanofilm bilayers (n = 2)	1902:1953	A minimum number of Col/AA nanofilm bilayers (n = 2) was required for effective induction of MSC osteogenic differentiation.
30594632	2	12	theme	native	349:354	arg1	tissue					356:361	the native tissue	345:361	the native tissue for gaining optimal function	345:390	Numerous studies have attempted to produce cellular components that mimic the native tissue for gaining optimal function.
30594632	1	13	theme	engineering	214:224	arg1	important					230:238	important	230:238	important	230:238	In regenerative medicine, the generation of therapeutic stem cells and tissue engineering are important for replacing damaged tissues.
30594632	1	13	theme	engineering	214:224	arg1	generation					166:175	the generation	162:175	the generation of therapeutic stem cells and tissue engineering	162:224	In regenerative medicine, the generation of therapeutic stem cells and tissue engineering are important for replacing damaged tissues.
30594632	12	14	theme	stiffness	2131:2139	arg1	effect					2090:2095	the promoting effect	2076:2095	the promoting effect of osteogenic differentiation and stiffness of the nanofilm	2076:2155	In addition, we observed the correlation between the promoting effect of osteogenic differentiation and stiffness of the nanofilm.
30594632	11	15	theme	Col/AA	1922:1927	arg1	bilayers					1938:1945	Col/AA nanofilm bilayers	1922:1945	Col/AA nanofilm bilayers (n = 2)	1922:1953	A minimum number of Col/AA nanofilm bilayers (n = 2) was required for effective induction of MSC osteogenic differentiation.
30594632	11	15	theme	Col/AA	1922:1927	arg1	n = 2					1948:1952	n = 2	1948:1952	n = 2	1948:1952	A minimum number of Col/AA nanofilm bilayers (n = 2) was required for effective induction of MSC osteogenic differentiation.
30594632	5	16	theme	high	855:858	arg1	stiffness					860:868	relatively high stiffness	844:868	relatively high stiffness	844:868	Interestingly, nanofilm composed of collagen (Col) and alginate (AA) showed relatively high stiffness and MSCs coated with the Col/AA nanofilm showed increased osteogenic differentiation efficiency compared to other nanofilm-coated MSCs.
30594632	12	17	theme	promoting	2080:2088	arg1	effect					2090:2095	the promoting effect	2076:2095	the promoting effect of osteogenic differentiation and stiffness of the nanofilm	2076:2155	In addition, we observed the correlation between the promoting effect of osteogenic differentiation and stiffness of the nanofilm.
30594632	10	18	theme	transcription	1774:1786	arg1	induction					1745:1753	the induction	1741:1753	the induction of osteogenic gene transcription in collagen (Col) and alginate (AA) film-coated MSCs	1741:1839	Among the various nanofilm combinations, the induction of osteogenic gene transcription in collagen (Col) and alginate (AA) film-coated MSCs was the most pronounced compared to that on other nanofilms.
30594632	10	18	theme	transcription	1774:1786	arg1	pronounced					1854:1863	pronounced	1854:1863	pronounced	1854:1863	Among the various nanofilm combinations, the induction of osteogenic gene transcription in collagen (Col) and alginate (AA) film-coated MSCs was the most pronounced compared to that on other nanofilms.
30594632	5	19	theme	differentiation	939:953	arg1	efficiency					955:964	increased osteogenic differentiation efficiency	918:964	increased osteogenic differentiation efficiency	918:964	Interestingly, nanofilm composed of collagen (Col) and alginate (AA) showed relatively high stiffness and MSCs coated with the Col/AA nanofilm showed increased osteogenic differentiation efficiency compared to other nanofilm-coated MSCs.
30594632	8	20	theme	SIGNIFICANCE	1431:1442	arg1	STATE					1422:1426	STATE	1422:1426	STATE OF SIGNIFICANCE	1422:1442	STATE OF SIGNIFICANCE: In this study, we developed an artificial cellular nano-environment formed by multilayer nanofilms.
30594632	0	21	theme	osteogenic	82:91	arg1	differentiation					93:107	osteogenic differentiation	82:107	osteogenic differentiation of mesenchymal stem cells	82:133	Artificial cellular nano-environment composed of collagen-based nanofilm promotes osteogenic differentiation of mesenchymal stem cells.
30594632	13	22	theme	cell	2201:2204	arg1	system					2220:2225	a cell coating model system	2199:2225	a cell coating model system widely applicable in bioengineering and regenerative medicine	2199:2287	Our results may be useful for developing a cell coating model system widely applicable in bioengineering and regenerative medicine.
30594632	4	23	theme	ECM	735:737	arg1	environment					739:749	an artificial ECM environment	721:749	an artificial ECM environment for single MSCs	721:765	Here, we evaluated the osteogenic effects of a nanofilm in which oppositely charged polyelectrolytes were alternately adsorbed onto the cell surface to create an artificial ECM environment for single MSCs.
30594632	7	24	theme	cellular	1326:1333	arg1	functions					1335:1343	cellular functions	1326:1343	cellular functions	1326:1343	This nano-sized cellular coating will facilitate the development of nanotechnology for controlling cellular functions and advance stem cell-based clinical applications for regenerative medicine.
30594632	13	25	theme	model	2214:2218	arg1	system					2220:2225	a cell coating model system	2199:2225	a cell coating model system widely applicable in bioengineering and regenerative medicine	2199:2287	Our results may be useful for developing a cell coating model system widely applicable in bioengineering and regenerative medicine.
30594632	1	26	theme	stem	192:195	arg1	cells					197:201	therapeutic stem cells	180:201	therapeutic stem cells	180:201	In regenerative medicine, the generation of therapeutic stem cells and tissue engineering are important for replacing damaged tissues.
30594632	6	27	theme	Col/AA	1041:1046	arg1	coating					1057:1063	the Col/AA nanofilm coating	1037:1063	the Col/AA nanofilm coating	1037:1063	Further analysis revealed that the Col/AA nanofilm coating stimulated osteogenesis by activating transcriptional coactivators with the PDZ binding motif through extracellular signal-related kinase and p38 MAPK signaling.
30594632	0	28	theme	stem	124:127	arg1	cells					129:133	mesenchymal stem cells	112:133	mesenchymal stem cells	112:133	Artificial cellular nano-environment composed of collagen-based nanofilm promotes osteogenic differentiation of mesenchymal stem cells.
30594632	0	29	theme	collagen-based	49:62	arg1	nanofilm					64:71	collagen-based nanofilm	49:71	collagen-based nanofilm	49:71	Artificial cellular nano-environment composed of collagen-based nanofilm promotes osteogenic differentiation of mesenchymal stem cells.
30594632	6	30	theme	binding	1145:1151	arg1	motif					1153:1157	the PDZ binding motif	1137:1157	the PDZ binding motif	1137:1157	Further analysis revealed that the Col/AA nanofilm coating stimulated osteogenesis by activating transcriptional coactivators with the PDZ binding motif through extracellular signal-related kinase and p38 MAPK signaling.
30594632	8	31	theme	cellular	1487:1494	arg1	nano-environment					1496:1511	an artificial cellular nano-environment	1473:1511	an artificial cellular nano-environment formed by multilayer nanofilms	1473:1542	STATE OF SIGNIFICANCE: In this study, we developed an artificial cellular nano-environment formed by multilayer nanofilms.
30594632	3	32	theme	important	459:467	arg1	role					469:472	an important role	456:472	an important role	456:472	Particularly, the extracellular matrix (ECM) composition plays an important role in cellular functions including determining the fates of mesenchymal stem cells (MSCs).
30594632	0	33	theme	Artificial	0:9	arg1	nano-environment					20:35	Artificial cellular nano-environment	0:35	Artificial cellular nano-environment composed of collagen-based nanofilm	0:71	Artificial cellular nano-environment composed of collagen-based nanofilm promotes osteogenic differentiation of mesenchymal stem cells.
30594632	5	34	theme	other	978:982	arg1	MSCs					1000:1003	other nanofilm-coated MSCs	978:1003	other nanofilm-coated MSCs	978:1003	Interestingly, nanofilm composed of collagen (Col) and alginate (AA) showed relatively high stiffness and MSCs coated with the Col/AA nanofilm showed increased osteogenic differentiation efficiency compared to other nanofilm-coated MSCs.
30594632	3	35	theme	extracellular	411:423	arg1	ECM					433:435	ECM	433:435	ECM	433:435	Particularly, the extracellular matrix (ECM) composition plays an important role in cellular functions including determining the fates of mesenchymal stem cells (MSCs).
30594632	3	35	theme	extracellular	411:423	arg1	matrix					425:430	extracellular matrix	411:430	the extracellular matrix (ECM) composition	407:448	Particularly, the extracellular matrix (ECM) composition plays an important role in cellular functions including determining the fates of mesenchymal stem cells (MSCs).
30594632	10	36	theme	film-coated	1824:1834	arg1	MSCs					1836:1839	film-coated MSCs	1824:1839	film-coated MSCs	1824:1839	Among the various nanofilm combinations, the induction of osteogenic gene transcription in collagen (Col) and alginate (AA) film-coated MSCs was the most pronounced compared to that on other nanofilms.
30594632	10	37	theme	other	1885:1889	arg1	nanofilms					1891:1899	other nanofilms	1885:1899	other nanofilms	1885:1899	Among the various nanofilm combinations, the induction of osteogenic gene transcription in collagen (Col) and alginate (AA) film-coated MSCs was the most pronounced compared to that on other nanofilms.
30594632	4	38	theme	osteogenic	585:594	arg1	effects					596:602	the osteogenic effects	581:602	the osteogenic effects of a nanofilm in which oppositely charged polyelectrolytes were alternately adsorbed onto the cell surface to create an artificial ECM environment for single MSCs	581:765	Here, we evaluated the osteogenic effects of a nanofilm in which oppositely charged polyelectrolytes were alternately adsorbed onto the cell surface to create an artificial ECM environment for single MSCs.
30594632	8	39	theme	multilayer	1523:1532	arg1	nanofilms					1534:1542	multilayer nanofilms	1523:1542	multilayer nanofilms	1523:1542	STATE OF SIGNIFICANCE: In this study, we developed an artificial cellular nano-environment formed by multilayer nanofilms.
30594632	7	40	theme	cellular	1243:1250	arg1	coating					1252:1258	This nano-sized cellular coating	1227:1258	This nano-sized cellular coating	1227:1258	This nano-sized cellular coating will facilitate the development of nanotechnology for controlling cellular functions and advance stem cell-based clinical applications for regenerative medicine.
30594632	3	41	theme	mesenchymal	531:541	arg1	MSCs					555:558	MSCs	555:558	MSCs	555:558	Particularly, the extracellular matrix (ECM) composition plays an important role in cellular functions including determining the fates of mesenchymal stem cells (MSCs).
30594632	3	41	theme	mesenchymal	531:541	arg1	cells					548:552	mesenchymal stem cells	531:552	mesenchymal stem cells (MSCs)	531:559	Particularly, the extracellular matrix (ECM) composition plays an important role in cellular functions including determining the fates of mesenchymal stem cells (MSCs).
30594632	6	42	theme	MAPK	1211:1214	arg1	signaling					1216:1224	extracellular signal-related kinase and p38 MAPK signaling	1167:1224	extracellular signal-related kinase and p38 MAPK signaling	1167:1224	Further analysis revealed that the Col/AA nanofilm coating stimulated osteogenesis by activating transcriptional coactivators with the PDZ binding motif through extracellular signal-related kinase and p38 MAPK signaling.
30594632	1	43	theme	damaged	254:260	arg1	tissues					262:268	damaged tissues	254:268	damaged tissues	254:268	In regenerative medicine, the generation of therapeutic stem cells and tissue engineering are important for replacing damaged tissues.
30594632	10	44	theme	various	1710:1716	arg1	combinations					1727:1738	the various nanofilm combinations	1706:1738	the various nanofilm combinations	1706:1738	Among the various nanofilm combinations, the induction of osteogenic gene transcription in collagen (Col) and alginate (AA) film-coated MSCs was the most pronounced compared to that on other nanofilms.
30594632	6	45	theme	signal-related	1181:1194	arg1	kinase					1196:1201	extracellular signal-related kinase	1167:1201	extracellular signal-related kinase	1167:1201	Further analysis revealed that the Col/AA nanofilm coating stimulated osteogenesis by activating transcriptional coactivators with the PDZ binding motif through extracellular signal-related kinase and p38 MAPK signaling.
30594632	11	46	theme	differentiation	2010:2024	arg1	induction					1982:1990	effective induction	1972:1990	effective induction of MSC osteogenic differentiation	1972:2024	A minimum number of Col/AA nanofilm bilayers (n = 2) was required for effective induction of MSC osteogenic differentiation.
30594632	7	47	theme	clinical	1373:1380	arg1	applications					1382:1393	stem cell-based clinical applications	1357:1393	stem cell-based clinical applications for regenerative medicine	1357:1419	This nano-sized cellular coating will facilitate the development of nanotechnology for controlling cellular functions and advance stem cell-based clinical applications for regenerative medicine.
30594632	4	48	theme	charged	638:644	arg1	polyelectrolytes					646:661	oppositely charged polyelectrolytes	627:661	oppositely charged polyelectrolytes	627:661	Here, we evaluated the osteogenic effects of a nanofilm in which oppositely charged polyelectrolytes were alternately adsorbed onto the cell surface to create an artificial ECM environment for single MSCs.
30594632	3	49	theme	cells	548:552	arg1	fates					522:526	the fates	518:526	the fates of mesenchymal stem cells (MSCs)	518:559	Particularly, the extracellular matrix (ECM) composition plays an important role in cellular functions including determining the fates of mesenchymal stem cells (MSCs).
30594632	5	50	dep	showed	837:842	arg1	showed					911:916	showed	911:916	showed relatively high stiffness and MSCs coated with the Col/AA nanofilm showed increased osteogenic differentiation efficiency compared to other nanofilm-coated MSCs	837:1003	Interestingly, nanofilm composed of collagen (Col) and alginate (AA) showed relatively high stiffness and MSCs coated with the Col/AA nanofilm showed increased osteogenic differentiation efficiency compared to other nanofilm-coated MSCs.
30594632	13	51	from	medicine	2280:2287	arg1	applicable					2234:2243	applicable	2234:2243	applicable	2234:2243	Our results may be useful for developing a cell coating model system widely applicable in bioengineering and regenerative medicine.
30594632	9	52	theme	intracellular	1675:1687	arg1	signaling					1689:1697	intracellular signaling	1675:1697	intracellular signaling	1675:1697	We demonstrated that the nanofilms introduced to mesenchymal stem cells (MSCs) stimulate osteogenic differentiation by regulating intracellular signaling.
30594632	4	53	theme	nanofilm	609:616	arg1	effects					596:602	the osteogenic effects	581:602	the osteogenic effects of a nanofilm in which oppositely charged polyelectrolytes were alternately adsorbed onto the cell surface to create an artificial ECM environment for single MSCs	581:765	Here, we evaluated the osteogenic effects of a nanofilm in which oppositely charged polyelectrolytes were alternately adsorbed onto the cell surface to create an artificial ECM environment for single MSCs.
30594632	2	54	theme	cellular	314:321	arg1	components					323:332	cellular components	314:332	cellular components that mimic the native tissue for gaining optimal function	314:390	Numerous studies have attempted to produce cellular components that mimic the native tissue for gaining optimal function.
30594632	1	55	theme	cells	197:201	arg1	important					230:238	important	230:238	important	230:238	In regenerative medicine, the generation of therapeutic stem cells and tissue engineering are important for replacing damaged tissues.
30594632	1	55	theme	cells	197:201	arg1	generation					166:175	the generation	162:175	the generation of therapeutic stem cells and tissue engineering	162:224	In regenerative medicine, the generation of therapeutic stem cells and tissue engineering are important for replacing damaged tissues.
30594632	12	56	theme	nanofilm	2148:2155	arg1	differentiation					2111:2125	differentiation	2111:2125	differentiation	2111:2125	In addition, we observed the correlation between the promoting effect of osteogenic differentiation and stiffness of the nanofilm.
30594632	12	56	theme	nanofilm	2148:2155	arg1	stiffness					2131:2139	stiffness	2131:2139	stiffness	2131:2139	In addition, we observed the correlation between the promoting effect of osteogenic differentiation and stiffness of the nanofilm.
30594632	10	57	theme	gene	1769:1772	arg1	transcription					1774:1786	osteogenic gene transcription	1758:1786	osteogenic gene transcription	1758:1786	Among the various nanofilm combinations, the induction of osteogenic gene transcription in collagen (Col) and alginate (AA) film-coated MSCs was the most pronounced compared to that on other nanofilms.
30594632	1	58	theme	tissue	207:212	arg1	engineering					214:224	tissue engineering	207:224	tissue engineering	207:224	In regenerative medicine, the generation of therapeutic stem cells and tissue engineering are important for replacing damaged tissues.
30594632	12	59	theme	osteogenic	2100:2109	arg1	differentiation					2111:2125	differentiation	2111:2125	differentiation	2111:2125	In addition, we observed the correlation between the promoting effect of osteogenic differentiation and stiffness of the nanofilm.
30594632	9	60	theme	stem	1606:1609	arg1	cells					1611:1615	mesenchymal stem cells	1594:1615	mesenchymal stem cells (MSCs)	1594:1622	We demonstrated that the nanofilms introduced to mesenchymal stem cells (MSCs) stimulate osteogenic differentiation by regulating intracellular signaling.
30594632	9	60	theme	stem	1606:1609	arg1	MSCs					1618:1621	MSCs	1618:1621	MSCs	1618:1621	We demonstrated that the nanofilms introduced to mesenchymal stem cells (MSCs) stimulate osteogenic differentiation by regulating intracellular signaling.
30594632	6	61	theme	Further	1006:1012	arg1	analysis					1014:1021	Further analysis	1006:1021	Further analysis	1006:1021	Further analysis revealed that the Col/AA nanofilm coating stimulated osteogenesis by activating transcriptional coactivators with the PDZ binding motif through extracellular signal-related kinase and p38 MAPK signaling.
30594632	5	62	theme	increased	918:926	arg1	efficiency					955:964	increased osteogenic differentiation efficiency	918:964	increased osteogenic differentiation efficiency	918:964	Interestingly, nanofilm composed of collagen (Col) and alginate (AA) showed relatively high stiffness and MSCs coated with the Col/AA nanofilm showed increased osteogenic differentiation efficiency compared to other nanofilm-coated MSCs.
30594632	3	63	theme	stem	543:546	arg1	MSCs					555:558	MSCs	555:558	MSCs	555:558	Particularly, the extracellular matrix (ECM) composition plays an important role in cellular functions including determining the fates of mesenchymal stem cells (MSCs).
30594632	3	63	theme	stem	543:546	arg1	cells					548:552	mesenchymal stem cells	531:552	mesenchymal stem cells (MSCs)	531:559	Particularly, the extracellular matrix (ECM) composition plays an important role in cellular functions including determining the fates of mesenchymal stem cells (MSCs).
30594632	13	64	theme	regenerative	2267:2278	arg1	medicine					2280:2287	regenerative medicine	2267:2287	regenerative medicine	2267:2287	Our results may be useful for developing a cell coating model system widely applicable in bioengineering and regenerative medicine.
30594632	2	65	theme	Numerous	271:278	arg1	studies					280:286	Numerous studies	271:286	Numerous studies	271:286	Numerous studies have attempted to produce cellular components that mimic the native tissue for gaining optimal function.
30594632	9	66	theme	mesenchymal	1594:1604	arg1	cells					1611:1615	mesenchymal stem cells	1594:1615	mesenchymal stem cells (MSCs)	1594:1622	We demonstrated that the nanofilms introduced to mesenchymal stem cells (MSCs) stimulate osteogenic differentiation by regulating intracellular signaling.
30594632	9	66	theme	mesenchymal	1594:1604	arg1	MSCs					1618:1621	MSCs	1618:1621	MSCs	1618:1621	We demonstrated that the nanofilms introduced to mesenchymal stem cells (MSCs) stimulate osteogenic differentiation by regulating intracellular signaling.
30594632	7	67	theme	nanotechnology	1295:1308	arg1	development					1280:1290	the development	1276:1290	the development of nanotechnology for controlling cellular functions	1276:1343	This nano-sized cellular coating will facilitate the development of nanotechnology for controlling cellular functions and advance stem cell-based clinical applications for regenerative medicine.
30594632	1	68	theme	regenerative	139:150	arg1	medicine					152:159	regenerative medicine	139:159	regenerative medicine	139:159	In regenerative medicine, the generation of therapeutic stem cells and tissue engineering are important for replacing damaged tissues.
30594632	13	69	theme	applicable	2234:2243	arg1	system					2220:2225	a cell coating model system	2199:2225	a cell coating model system widely applicable in bioengineering and regenerative medicine	2199:2287	Our results may be useful for developing a cell coating model system widely applicable in bioengineering and regenerative medicine.
30594632	1	70	from	important	230:238	arg1	medicine					152:159	regenerative medicine	139:159	regenerative medicine	139:159	In regenerative medicine, the generation of therapeutic stem cells and tissue engineering are important for replacing damaged tissues.
30594632	9	71	theme	osteogenic	1634:1643	arg1	differentiation					1645:1659	osteogenic differentiation	1634:1659	osteogenic differentiation	1634:1659	We demonstrated that the nanofilms introduced to mesenchymal stem cells (MSCs) stimulate osteogenic differentiation by regulating intracellular signaling.
30594632	4	72	theme	single	755:760	arg1	MSCs					762:765	single MSCs	755:765	single MSCs	755:765	Here, we evaluated the osteogenic effects of a nanofilm in which oppositely charged polyelectrolytes were alternately adsorbed onto the cell surface to create an artificial ECM environment for single MSCs.
30594632	5	73	theme	Col/AA	895:900	arg1	nanofilm					902:909	the Col/AA nanofilm	891:909	the Col/AA nanofilm	891:909	Interestingly, nanofilm composed of collagen (Col) and alginate (AA) showed relatively high stiffness and MSCs coated with the Col/AA nanofilm showed increased osteogenic differentiation efficiency compared to other nanofilm-coated MSCs.
30594632	0	74	theme	mesenchymal	112:122	arg1	cells					129:133	mesenchymal stem cells	112:133	mesenchymal stem cells	112:133	Artificial cellular nano-environment composed of collagen-based nanofilm promotes osteogenic differentiation of mesenchymal stem cells.
30594632	13	75	theme	coating	2206:2212	arg1	system					2220:2225	a cell coating model system	2199:2225	a cell coating model system widely applicable in bioengineering and regenerative medicine	2199:2287	Our results may be useful for developing a cell coating model system widely applicable in bioengineering and regenerative medicine.
30594632	1	76	theme	therapeutic	180:190	arg1	cells					197:201	therapeutic stem cells	180:201	therapeutic stem cells	180:201	In regenerative medicine, the generation of therapeutic stem cells and tissue engineering are important for replacing damaged tissues.
30594632	6	77	theme	nanofilm	1048:1055	arg1	coating					1057:1063	the Col/AA nanofilm coating	1037:1063	the Col/AA nanofilm coating	1037:1063	Further analysis revealed that the Col/AA nanofilm coating stimulated osteogenesis by activating transcriptional coactivators with the PDZ binding motif through extracellular signal-related kinase and p38 MAPK signaling.
30594632	4	78	theme	artificial	724:733	arg1	environment					739:749	an artificial ECM environment	721:749	an artificial ECM environment for single MSCs	721:765	Here, we evaluated the osteogenic effects of a nanofilm in which oppositely charged polyelectrolytes were alternately adsorbed onto the cell surface to create an artificial ECM environment for single MSCs.
30594632	0	79	theme	cells	129:133	arg1	differentiation					93:107	osteogenic differentiation	82:107	osteogenic differentiation of mesenchymal stem cells	82:133	Artificial cellular nano-environment composed of collagen-based nanofilm promotes osteogenic differentiation of mesenchymal stem cells.
30594632	11	80	theme	minimum	1904:1910	arg1	number					1912:1917	A minimum number	1902:1917	A minimum number of Col/AA nanofilm bilayers (n = 2)	1902:1953	A minimum number of Col/AA nanofilm bilayers (n = 2) was required for effective induction of MSC osteogenic differentiation.
30594632	3	81	theme	cellular	477:484	arg1	functions					486:494	cellular functions	477:494	cellular functions including determining the fates of mesenchymal stem cells (MSCs)	477:559	Particularly, the extracellular matrix (ECM) composition plays an important role in cellular functions including determining the fates of mesenchymal stem cells (MSCs).
30594632	0	82	theme	cellular	11:18	arg1	nano-environment					20:35	Artificial cellular nano-environment	0:35	Artificial cellular nano-environment composed of collagen-based nanofilm	0:71	Artificial cellular nano-environment composed of collagen-based nanofilm promotes osteogenic differentiation of mesenchymal stem cells.
30594632	11	83	theme	nanofilm	1929:1936	arg1	bilayers					1938:1945	Col/AA nanofilm bilayers	1922:1945	Col/AA nanofilm bilayers (n = 2)	1922:1953	A minimum number of Col/AA nanofilm bilayers (n = 2) was required for effective induction of MSC osteogenic differentiation.
30594632	11	83	theme	nanofilm	1929:1936	arg1	n = 2					1948:1952	n = 2	1948:1952	n = 2	1948:1952	A minimum number of Col/AA nanofilm bilayers (n = 2) was required for effective induction of MSC osteogenic differentiation.
30594632	6	84	theme	PDZ	1141:1143	arg1	motif					1153:1157	the PDZ binding motif	1137:1157	the PDZ binding motif	1137:1157	Further analysis revealed that the Col/AA nanofilm coating stimulated osteogenesis by activating transcriptional coactivators with the PDZ binding motif through extracellular signal-related kinase and p38 MAPK signaling.
30594632	8	85	theme	artificial	1476:1485	arg1	nano-environment					1496:1511	an artificial cellular nano-environment	1473:1511	an artificial cellular nano-environment formed by multilayer nanofilms	1473:1542	STATE OF SIGNIFICANCE: In this study, we developed an artificial cellular nano-environment formed by multilayer nanofilms.
30594632	1	86	from	medicine	152:159	arg1	important					230:238	important	230:238	important	230:238	In regenerative medicine, the generation of therapeutic stem cells and tissue engineering are important for replacing damaged tissues.
30594632	1	86	from	medicine	152:159	arg1	generation					166:175	the generation	162:175	the generation of therapeutic stem cells and tissue engineering	162:224	In regenerative medicine, the generation of therapeutic stem cells and tissue engineering are important for replacing damaged tissues.
30594632	5	87	theme	nanofilm-coated	984:998	arg1	MSCs					1000:1003	other nanofilm-coated MSCs	978:1003	other nanofilm-coated MSCs	978:1003	Interestingly, nanofilm composed of collagen (Col) and alginate (AA) showed relatively high stiffness and MSCs coated with the Col/AA nanofilm showed increased osteogenic differentiation efficiency compared to other nanofilm-coated MSCs.
30594632	3	88	theme	matrix	425:430	arg1	composition					438:448	the extracellular matrix (ECM) composition	407:448	the extracellular matrix (ECM) composition	407:448	Particularly, the extracellular matrix (ECM) composition plays an important role in cellular functions including determining the fates of mesenchymal stem cells (MSCs).
30594632	6	89	theme	kinase	1196:1201	arg1	signaling					1216:1224	extracellular signal-related kinase and p38 MAPK signaling	1167:1224	extracellular signal-related kinase and p38 MAPK signaling	1167:1224	Further analysis revealed that the Col/AA nanofilm coating stimulated osteogenesis by activating transcriptional coactivators with the PDZ binding motif through extracellular signal-related kinase and p38 MAPK signaling.
30594632	7	90	theme	nano-sized	1232:1241	arg1	coating					1252:1258	This nano-sized cellular coating	1227:1258	This nano-sized cellular coating	1227:1258	This nano-sized cellular coating will facilitate the development of nanotechnology for controlling cellular functions and advance stem cell-based clinical applications for regenerative medicine.
30594632	6	91	theme	transcriptional	1103:1117	arg1	coactivators					1119:1130	transcriptional coactivators	1103:1130	transcriptional coactivators with the PDZ binding motif	1103:1157	Further analysis revealed that the Col/AA nanofilm coating stimulated osteogenesis by activating transcriptional coactivators with the PDZ binding motif through extracellular signal-related kinase and p38 MAPK signaling.
30594632	7	92	theme	regenerative	1399:1410	arg1	medicine					1412:1419	regenerative medicine	1399:1419	regenerative medicine	1399:1419	This nano-sized cellular coating will facilitate the development of nanotechnology for controlling cellular functions and advance stem cell-based clinical applications for regenerative medicine.
30594632	2	93	theme	optimal	375:381	arg1	function					383:390	optimal function	375:390	optimal function	375:390	Numerous studies have attempted to produce cellular components that mimic the native tissue for gaining optimal function.
30594632	6	94	theme	p38	1207:1209	arg1	MAPK					1211:1214	p38 MAPK	1207:1214	p38 MAPK	1207:1214	Further analysis revealed that the Col/AA nanofilm coating stimulated osteogenesis by activating transcriptional coactivators with the PDZ binding motif through extracellular signal-related kinase and p38 MAPK signaling.
30594632	11	95	theme	effective	1972:1980	arg1	induction					1982:1990	effective induction	1972:1990	effective induction of MSC osteogenic differentiation	1972:2024	A minimum number of Col/AA nanofilm bilayers (n = 2) was required for effective induction of MSC osteogenic differentiation.
30594632	6	96	with	coactivators	1119:1130	arg1	motif					1153:1157	the PDZ binding motif	1137:1157	the PDZ binding motif	1137:1157	Further analysis revealed that the Col/AA nanofilm coating stimulated osteogenesis by activating transcriptional coactivators with the PDZ binding motif through extracellular signal-related kinase and p38 MAPK signaling.
30594632	7	97	theme	cell-based	1362:1371	arg1	applications					1382:1393	stem cell-based clinical applications	1357:1393	stem cell-based clinical applications for regenerative medicine	1357:1419	This nano-sized cellular coating will facilitate the development of nanotechnology for controlling cellular functions and advance stem cell-based clinical applications for regenerative medicine.
30594632	13	98	from	bioengineering	2248:2261	arg1	applicable					2234:2243	applicable	2234:2243	applicable	2234:2243	Our results may be useful for developing a cell coating model system widely applicable in bioengineering and regenerative medicine.
31748622	0	0	theme	acylsugar	64:72	arg1	diversity					83:91	tomato acylsugar chemical diversity	57:91	tomato acylsugar chemical diversity	57:91	A thrips vector of tomato spotted wilt virus responds to tomato acylsugar chemical diversity with reduced oviposition and virus inoculation.
31748622	3	1	theme	profiles	638:645	arg1	amount					598:603	acylsucrose amount	586:603	acylsucrose amount	586:603	We sought to understand how acylsucrose amount and composition of associated FA profiles interacted to mediate resistance to WFT oviposition and TSWV inoculation on tomato leaves.
31748622	3	1	theme	profiles	638:645	arg1	profiles					638:645	associated FA profiles	624:645	associated FA profiles	624:645	We sought to understand how acylsucrose amount and composition of associated FA profiles interacted to mediate resistance to WFT oviposition and TSWV inoculation on tomato leaves.
31748622	3	1	theme	profiles	638:645	arg1	composition					609:619	composition	609:619	composition	609:619	We sought to understand how acylsucrose amount and composition of associated FA profiles interacted to mediate resistance to WFT oviposition and TSWV inoculation on tomato leaves.
31748622	3	2	dep	tomato	723:728	arg1	leaves					730:735	leaves	730:735	leaves	730:735	We sought to understand how acylsucrose amount and composition of associated FA profiles interacted to mediate resistance to WFT oviposition and TSWV inoculation on tomato leaves.
31748622	0	3	theme	tomato	57:62	arg1	diversity					83:91	tomato acylsugar chemical diversity	57:91	tomato acylsugar chemical diversity	57:91	A thrips vector of tomato spotted wilt virus responds to tomato acylsugar chemical diversity with reduced oviposition and virus inoculation.
31748622	4	4	from	behavior	806:813	arg1	lines					825:829	tomato lines	818:829	tomato lines bred to exude varying amounts of acylsucrose in association with diverse FA profiles	818:914	Towards this goal, we examined WFT oviposition and TSWV inoculation behavior on tomato lines bred to exude varying amounts of acylsucrose in association with diverse FA profiles.
31748622	3	5	from	inoculation	708:718	arg1	tomato					723:728	tomato leaves	723:735	tomato leaves	723:735	We sought to understand how acylsucrose amount and composition of associated FA profiles interacted to mediate resistance to WFT oviposition and TSWV inoculation on tomato leaves.
31748622	1	6	theme	increasing	150:159	arg1	evidence					161:168	increasing evidence that acylsugars deter insect pests and plant virus vectors, including the western flower thrips (WFT), Frankliniella occidentalis (Pergande), vector of tomato spotted wilt virus (TSWV)	150:353	increasing evidence that acylsugars deter insect pests and plant virus vectors, including the western flower thrips (WFT), Frankliniella occidentalis (Pergande), vector of tomato spotted wilt virus (TSWV)	150:353	There is increasing evidence that acylsugars deter insect pests and plant virus vectors, including the western flower thrips (WFT), Frankliniella occidentalis (Pergande), vector of tomato spotted wilt virus (TSWV).
31748622	1	7	theme	western	244:250	arg1	vector					312:317	vector	312:317	vector of tomato spotted wilt virus (TSWV)	312:353	There is increasing evidence that acylsugars deter insect pests and plant virus vectors, including the western flower thrips (WFT), Frankliniella occidentalis (Pergande), vector of tomato spotted wilt virus (TSWV).
31748622	1	7	theme	western	244:250	arg1	occidentalis					287:298	Frankliniella occidentalis	273:298	Frankliniella occidentalis (Pergande)	273:309	There is increasing evidence that acylsugars deter insect pests and plant virus vectors, including the western flower thrips (WFT), Frankliniella occidentalis (Pergande), vector of tomato spotted wilt virus (TSWV).
31748622	1	7	theme	western	244:250	arg1	WFT					267:269	WFT	267:269	WFT	267:269	There is increasing evidence that acylsugars deter insect pests and plant virus vectors, including the western flower thrips (WFT), Frankliniella occidentalis (Pergande), vector of tomato spotted wilt virus (TSWV).
31748622	1	7	theme	western	244:250	arg1	thrips					259:264	the western flower thrips	240:264	the western flower thrips (WFT)	240:270	There is increasing evidence that acylsugars deter insect pests and plant virus vectors, including the western flower thrips (WFT), Frankliniella occidentalis (Pergande), vector of tomato spotted wilt virus (TSWV).
31748622	3	8	theme	associated	624:633	arg1	profiles					638:645	associated FA profiles	624:645	associated FA profiles	624:645	We sought to understand how acylsucrose amount and composition of associated FA profiles interacted to mediate resistance to WFT oviposition and TSWV inoculation on tomato leaves.
31748622	4	9	theme	tomato	818:823	arg1	lines					825:829	tomato lines	818:829	tomato lines bred to exude varying amounts of acylsucrose in association with diverse FA profiles	818:914	Towards this goal, we examined WFT oviposition and TSWV inoculation behavior on tomato lines bred to exude varying amounts of acylsucrose in association with diverse FA profiles.
31748622	8	10	theme	type	1367:1370	arg1	trichomes					1375:1383	type IV trichomes	1367:1383	type IV trichomes	1367:1383	We show that increasing acylsucrose amount output by type IV trichomes and selecting for particular FA profiles through advanced breeding profoundly affects WFT behavior in ways that benefit management of WFT as direct pests and as TSWV vectors.
31748622	8	11	theme	IV	1372:1373	arg1	trichomes					1375:1383	type IV trichomes	1367:1383	type IV trichomes	1367:1383	We show that increasing acylsucrose amount output by type IV trichomes and selecting for particular FA profiles through advanced breeding profoundly affects WFT behavior in ways that benefit management of WFT as direct pests and as TSWV vectors.
31748622	1	12	theme	flower	252:257	arg1	vector					312:317	vector	312:317	vector of tomato spotted wilt virus (TSWV)	312:353	There is increasing evidence that acylsugars deter insect pests and plant virus vectors, including the western flower thrips (WFT), Frankliniella occidentalis (Pergande), vector of tomato spotted wilt virus (TSWV).
31748622	1	12	theme	flower	252:257	arg1	occidentalis					287:298	Frankliniella occidentalis	273:298	Frankliniella occidentalis (Pergande)	273:309	There is increasing evidence that acylsugars deter insect pests and plant virus vectors, including the western flower thrips (WFT), Frankliniella occidentalis (Pergande), vector of tomato spotted wilt virus (TSWV).
31748622	1	12	theme	flower	252:257	arg1	WFT					267:269	WFT	267:269	WFT	267:269	There is increasing evidence that acylsugars deter insect pests and plant virus vectors, including the western flower thrips (WFT), Frankliniella occidentalis (Pergande), vector of tomato spotted wilt virus (TSWV).
31748622	1	12	theme	flower	252:257	arg1	thrips					259:264	the western flower thrips	240:264	the western flower thrips (WFT)	240:270	There is increasing evidence that acylsugars deter insect pests and plant virus vectors, including the western flower thrips (WFT), Frankliniella occidentalis (Pergande), vector of tomato spotted wilt virus (TSWV).
31748622	0	13	theme	chemical	74:81	arg1	diversity					83:91	tomato acylsugar chemical diversity	57:91	tomato acylsugar chemical diversity	57:91	A thrips vector of tomato spotted wilt virus responds to tomato acylsugar chemical diversity with reduced oviposition and virus inoculation.
31748622	1	14	theme	tomato	322:327	arg1	TSWV					349:352	TSWV	349:352	TSWV	349:352	There is increasing evidence that acylsugars deter insect pests and plant virus vectors, including the western flower thrips (WFT), Frankliniella occidentalis (Pergande), vector of tomato spotted wilt virus (TSWV).
31748622	1	14	theme	tomato	322:327	arg1	virus					342:346	tomato spotted wilt virus	322:346	tomato spotted wilt virus (TSWV)	322:353	There is increasing evidence that acylsugars deter insect pests and plant virus vectors, including the western flower thrips (WFT), Frankliniella occidentalis (Pergande), vector of tomato spotted wilt virus (TSWV).
31748622	0	15	theme	reduced	98:104	arg1	oviposition					106:116	reduced oviposition	98:116	reduced oviposition	98:116	A thrips vector of tomato spotted wilt virus responds to tomato acylsugar chemical diversity with reduced oviposition and virus inoculation.
31748622	1	16	theme	spotted	329:335	arg1	TSWV					349:352	TSWV	349:352	TSWV	349:352	There is increasing evidence that acylsugars deter insect pests and plant virus vectors, including the western flower thrips (WFT), Frankliniella occidentalis (Pergande), vector of tomato spotted wilt virus (TSWV).
31748622	1	16	theme	spotted	329:335	arg1	virus					342:346	tomato spotted wilt virus	322:346	tomato spotted wilt virus (TSWV)	322:353	There is increasing evidence that acylsugars deter insect pests and plant virus vectors, including the western flower thrips (WFT), Frankliniella occidentalis (Pergande), vector of tomato spotted wilt virus (TSWV).
31748622	8	17	theme	particular	1403:1412	arg1	profiles					1417:1424	particular FA profiles	1403:1424	particular FA profiles	1403:1424	We show that increasing acylsucrose amount output by type IV trichomes and selecting for particular FA profiles through advanced breeding profoundly affects WFT behavior in ways that benefit management of WFT as direct pests and as TSWV vectors.
31748622	5	18	theme	TSWV	1013:1016	arg1	inoculation					1018:1028	TSWV inoculation	1013:1028	TSWV inoculation	1013:1028	Our data show that as acylsucrose amounts increased, WFT egg-laying (oviposition) decreased and TSWV inoculation was suppressed.
31748622	2	19	theme	fatty	469:473	arg1	acids					475:479	saturated, un-saturated, and variously branched short and long chain fatty acids	400:479	saturated, un-saturated, and variously branched short and long chain fatty acids (FAs) esterified to a glucose (acylglucose) or sucrose (acylsucrose) moiety	400:555	Acylsugars are sugar-polyesters composed of saturated, un-saturated, and variously branched short and long chain fatty acids (FAs) esterified to a glucose (acylglucose) or sucrose (acylsucrose) moiety.
31748622	2	19	theme	fatty	469:473	arg1	FAs					482:484	FAs	482:484	FAs	482:484	Acylsugars are sugar-polyesters composed of saturated, un-saturated, and variously branched short and long chain fatty acids (FAs) esterified to a glucose (acylglucose) or sucrose (acylsucrose) moiety.
31748622	1	20	theme	wilt	337:340	arg1	TSWV					349:352	TSWV	349:352	TSWV	349:352	There is increasing evidence that acylsugars deter insect pests and plant virus vectors, including the western flower thrips (WFT), Frankliniella occidentalis (Pergande), vector of tomato spotted wilt virus (TSWV).
31748622	1	20	theme	wilt	337:340	arg1	virus					342:346	tomato spotted wilt virus	322:346	tomato spotted wilt virus (TSWV)	322:353	There is increasing evidence that acylsugars deter insect pests and plant virus vectors, including the western flower thrips (WFT), Frankliniella occidentalis (Pergande), vector of tomato spotted wilt virus (TSWV).
31748622	2	21	theme	branched	439:446	arg1	acids					475:479	saturated, un-saturated, and variously branched short and long chain fatty acids	400:479	saturated, un-saturated, and variously branched short and long chain fatty acids (FAs) esterified to a glucose (acylglucose) or sucrose (acylsucrose) moiety	400:555	Acylsugars are sugar-polyesters composed of saturated, un-saturated, and variously branched short and long chain fatty acids (FAs) esterified to a glucose (acylglucose) or sucrose (acylsucrose) moiety.
31748622	2	21	theme	branched	439:446	arg1	FAs					482:484	FAs	482:484	FAs	482:484	Acylsugars are sugar-polyesters composed of saturated, un-saturated, and variously branched short and long chain fatty acids (FAs) esterified to a glucose (acylglucose) or sucrose (acylsucrose) moiety.
31748622	8	22	theme	FA	1414:1415	arg1	profiles					1417:1424	particular FA profiles	1403:1424	particular FA profiles	1403:1424	We show that increasing acylsucrose amount output by type IV trichomes and selecting for particular FA profiles through advanced breeding profoundly affects WFT behavior in ways that benefit management of WFT as direct pests and as TSWV vectors.
31748622	4	23	theme	FA	904:905	arg1	profiles					907:914	diverse FA profiles	896:914	diverse FA profiles	896:914	Towards this goal, we examined WFT oviposition and TSWV inoculation behavior on tomato lines bred to exude varying amounts of acylsucrose in association with diverse FA profiles.
31748622	0	24	theme	thrips	2:7	arg1	vector					9:14	A thrips vector	0:14	A thrips vector of tomato spotted wilt virus	0:43	A thrips vector of tomato spotted wilt virus responds to tomato acylsugar chemical diversity with reduced oviposition and virus inoculation.
31748622	1	25	theme	virus	342:346	arg1	vector					312:317	vector	312:317	vector of tomato spotted wilt virus (TSWV)	312:353	There is increasing evidence that acylsugars deter insect pests and plant virus vectors, including the western flower thrips (WFT), Frankliniella occidentalis (Pergande), vector of tomato spotted wilt virus (TSWV).
31748622	1	25	theme	virus	342:346	arg1	thrips					259:264	the western flower thrips	240:264	the western flower thrips (WFT)	240:270	There is increasing evidence that acylsugars deter insect pests and plant virus vectors, including the western flower thrips (WFT), Frankliniella occidentalis (Pergande), vector of tomato spotted wilt virus (TSWV).
31748622	4	26	theme	TSWV	789:792	arg1	behavior					806:813	TSWV inoculation behavior	789:813	TSWV inoculation behavior	789:813	Towards this goal, we examined WFT oviposition and TSWV inoculation behavior on tomato lines bred to exude varying amounts of acylsucrose in association with diverse FA profiles.
31748622	8	27	theme	WFT	1471:1473	arg1	behavior					1475:1482	WFT behavior	1471:1482	WFT behavior	1471:1482	We show that increasing acylsucrose amount output by type IV trichomes and selecting for particular FA profiles through advanced breeding profoundly affects WFT behavior in ways that benefit management of WFT as direct pests and as TSWV vectors.
31748622	8	28	theme	advanced	1434:1441	arg1	breeding					1443:1450	advanced breeding	1434:1450	advanced breeding	1434:1450	We show that increasing acylsucrose amount output by type IV trichomes and selecting for particular FA profiles through advanced breeding profoundly affects WFT behavior in ways that benefit management of WFT as direct pests and as TSWV vectors.
31748622	6	29	theme	FA	1086:1087	arg1	profiles					1089:1096	FA profiles	1086:1096	FA profiles that included iC4, iC11, nC12 and nC10 FA	1086:1138	Western flower thrips also responded to FA profiles that included iC4, iC11, nC12 and nC10 FA.
31748622	1	30	theme	deter	186:190	arg1	pests					199:203	deter insect pests	186:203	deter insect pests	186:203	There is increasing evidence that acylsugars deter insect pests and plant virus vectors, including the western flower thrips (WFT), Frankliniella occidentalis (Pergande), vector of tomato spotted wilt virus (TSWV).
31748622	1	30	theme	deter	186:190	arg1	thrips					259:264	the western flower thrips	240:264	the western flower thrips (WFT)	240:270	There is increasing evidence that acylsugars deter insect pests and plant virus vectors, including the western flower thrips (WFT), Frankliniella occidentalis (Pergande), vector of tomato spotted wilt virus (TSWV).
31748622	2	31	theme	saturated	400:408	arg1	acids					475:479	saturated, un-saturated, and variously branched short and long chain fatty acids	400:479	saturated, un-saturated, and variously branched short and long chain fatty acids (FAs) esterified to a glucose (acylglucose) or sucrose (acylsucrose) moiety	400:555	Acylsugars are sugar-polyesters composed of saturated, un-saturated, and variously branched short and long chain fatty acids (FAs) esterified to a glucose (acylglucose) or sucrose (acylsucrose) moiety.
31748622	2	31	theme	saturated	400:408	arg1	FAs					482:484	FAs	482:484	FAs	482:484	Acylsugars are sugar-polyesters composed of saturated, un-saturated, and variously branched short and long chain fatty acids (FAs) esterified to a glucose (acylglucose) or sucrose (acylsucrose) moiety.
31748622	6	32	theme	nC10	1132:1135	arg1	FA					1137:1138	nC10 FA	1132:1138	nC10 FA	1132:1138	Western flower thrips also responded to FA profiles that included iC4, iC11, nC12 and nC10 FA.
31748622	4	33	theme	inoculation	794:804	arg1	behavior					806:813	TSWV inoculation behavior	789:813	TSWV inoculation behavior	789:813	Towards this goal, we examined WFT oviposition and TSWV inoculation behavior on tomato lines bred to exude varying amounts of acylsucrose in association with diverse FA profiles.
31748622	8	34	theme	acylsucrose	1338:1348	arg1	output					1357:1362	acylsucrose amount output	1338:1362	acylsucrose amount output by type IV trichomes	1338:1383	We show that increasing acylsucrose amount output by type IV trichomes and selecting for particular FA profiles through advanced breeding profoundly affects WFT behavior in ways that benefit management of WFT as direct pests and as TSWV vectors.
31748622	1	35	theme	insect	192:197	arg1	pests					199:203	deter insect pests	186:203	deter insect pests	186:203	There is increasing evidence that acylsugars deter insect pests and plant virus vectors, including the western flower thrips (WFT), Frankliniella occidentalis (Pergande), vector of tomato spotted wilt virus (TSWV).
31748622	1	35	theme	insect	192:197	arg1	thrips					259:264	the western flower thrips	240:264	the western flower thrips (WFT)	240:270	There is increasing evidence that acylsugars deter insect pests and plant virus vectors, including the western flower thrips (WFT), Frankliniella occidentalis (Pergande), vector of tomato spotted wilt virus (TSWV).
31748622	8	36	theme	WFT	1519:1521	arg1	vectors					1551:1557	as TSWV vectors	1543:1557	as TSWV vectors	1543:1557	We show that increasing acylsucrose amount output by type IV trichomes and selecting for particular FA profiles through advanced breeding profoundly affects WFT behavior in ways that benefit management of WFT as direct pests and as TSWV vectors.
31748622	8	36	theme	WFT	1519:1521	arg1	pests					1533:1537	direct pests	1526:1537	direct pests	1526:1537	We show that increasing acylsucrose amount output by type IV trichomes and selecting for particular FA profiles through advanced breeding profoundly affects WFT behavior in ways that benefit management of WFT as direct pests and as TSWV vectors.
31748622	8	36	theme	WFT	1519:1521	arg1	management					1505:1514	management	1505:1514	management of WFT	1505:1521	We show that increasing acylsucrose amount output by type IV trichomes and selecting for particular FA profiles through advanced breeding profoundly affects WFT behavior in ways that benefit management of WFT as direct pests and as TSWV vectors.
31748622	4	37	theme	varying	845:851	arg1	amounts					853:859	varying amounts	845:859	varying amounts of acylsucrose	845:874	Towards this goal, we examined WFT oviposition and TSWV inoculation behavior on tomato lines bred to exude varying amounts of acylsucrose in association with diverse FA profiles.
31748622	4	37	theme	varying	845:851	arg1	acylsucrose					864:874	acylsucrose	864:874	acylsucrose	864:874	Towards this goal, we examined WFT oviposition and TSWV inoculation behavior on tomato lines bred to exude varying amounts of acylsucrose in association with diverse FA profiles.
31748622	7	38	theme	greater	1245:1251	arg1	acylsucrose					1264:1274	acylsucrose	1264:1274	acylsucrose associated with specific FA profiles	1264:1311	These findings support improving acylsugar-mediated resistance against WFT by breeding tomatoes exuding greater amounts of acylsucrose associated with specific FA profiles.
31748622	7	38	theme	greater	1245:1251	arg1	amounts					1253:1259	greater amounts	1245:1259	greater amounts of acylsucrose associated with specific FA profiles	1245:1311	These findings support improving acylsugar-mediated resistance against WFT by breeding tomatoes exuding greater amounts of acylsucrose associated with specific FA profiles.
31748622	0	39	theme	spotted	26:32	arg1	virus					39:43	tomato spotted wilt virus	19:43	tomato spotted wilt virus	19:43	A thrips vector of tomato spotted wilt virus responds to tomato acylsugar chemical diversity with reduced oviposition and virus inoculation.
31748622	3	40	theme	WFT	683:685	arg1	oviposition					687:697	WFT oviposition	683:697	WFT oviposition	683:697	We sought to understand how acylsucrose amount and composition of associated FA profiles interacted to mediate resistance to WFT oviposition and TSWV inoculation on tomato leaves.
31748622	4	41	theme	acylsucrose	864:874	arg1	amounts					853:859	varying amounts	845:859	varying amounts of acylsucrose	845:874	Towards this goal, we examined WFT oviposition and TSWV inoculation behavior on tomato lines bred to exude varying amounts of acylsucrose in association with diverse FA profiles.
31748622	4	41	theme	acylsucrose	864:874	arg1	acylsucrose					864:874	acylsucrose	864:874	acylsucrose	864:874	Towards this goal, we examined WFT oviposition and TSWV inoculation behavior on tomato lines bred to exude varying amounts of acylsucrose in association with diverse FA profiles.
31748622	5	42	theme	acylsucrose	939:949	arg1	amounts					951:957	acylsucrose amounts	939:957	acylsucrose amounts	939:957	Our data show that as acylsucrose amounts increased, WFT egg-laying (oviposition) decreased and TSWV inoculation was suppressed.
31748622	2	43	theme	short	448:452	arg1	acids					475:479	saturated, un-saturated, and variously branched short and long chain fatty acids	400:479	saturated, un-saturated, and variously branched short and long chain fatty acids (FAs) esterified to a glucose (acylglucose) or sucrose (acylsucrose) moiety	400:555	Acylsugars are sugar-polyesters composed of saturated, un-saturated, and variously branched short and long chain fatty acids (FAs) esterified to a glucose (acylglucose) or sucrose (acylsucrose) moiety.
31748622	2	43	theme	short	448:452	arg1	FAs					482:484	FAs	482:484	FAs	482:484	Acylsugars are sugar-polyesters composed of saturated, un-saturated, and variously branched short and long chain fatty acids (FAs) esterified to a glucose (acylglucose) or sucrose (acylsucrose) moiety.
31748622	0	44	theme	tomato	19:24	arg1	virus					39:43	tomato spotted wilt virus	19:43	tomato spotted wilt virus	19:43	A thrips vector of tomato spotted wilt virus responds to tomato acylsugar chemical diversity with reduced oviposition and virus inoculation.
31748622	1	45	theme	Frankliniella	273:285	arg1	occidentalis					287:298	Frankliniella occidentalis	273:298	Frankliniella occidentalis (Pergande)	273:309	There is increasing evidence that acylsugars deter insect pests and plant virus vectors, including the western flower thrips (WFT), Frankliniella occidentalis (Pergande), vector of tomato spotted wilt virus (TSWV).
31748622	1	45	theme	Frankliniella	273:285	arg1	thrips					259:264	the western flower thrips	240:264	the western flower thrips (WFT)	240:270	There is increasing evidence that acylsugars deter insect pests and plant virus vectors, including the western flower thrips (WFT), Frankliniella occidentalis (Pergande), vector of tomato spotted wilt virus (TSWV).
31748622	1	45	theme	Frankliniella	273:285	arg1	Pergande					301:308	Pergande	301:308	Pergande	301:308	There is increasing evidence that acylsugars deter insect pests and plant virus vectors, including the western flower thrips (WFT), Frankliniella occidentalis (Pergande), vector of tomato spotted wilt virus (TSWV).
31748622	0	46	theme	virus	122:126	arg1	inoculation					128:138	virus inoculation	122:138	virus inoculation	122:138	A thrips vector of tomato spotted wilt virus responds to tomato acylsugar chemical diversity with reduced oviposition and virus inoculation.
31748622	7	47	theme	acylsucrose	1264:1274	arg1	acylsucrose					1264:1274	acylsucrose	1264:1274	acylsucrose associated with specific FA profiles	1264:1311	These findings support improving acylsugar-mediated resistance against WFT by breeding tomatoes exuding greater amounts of acylsucrose associated with specific FA profiles.
31748622	7	47	theme	acylsucrose	1264:1274	arg1	amounts					1253:1259	greater amounts	1245:1259	greater amounts of acylsucrose associated with specific FA profiles	1245:1311	These findings support improving acylsugar-mediated resistance against WFT by breeding tomatoes exuding greater amounts of acylsucrose associated with specific FA profiles.
31748622	1	48	theme	plant	209:213	arg1	vectors					221:227	plant virus vectors	209:227	plant virus vectors	209:227	There is increasing evidence that acylsugars deter insect pests and plant virus vectors, including the western flower thrips (WFT), Frankliniella occidentalis (Pergande), vector of tomato spotted wilt virus (TSWV).
31748622	1	48	theme	plant	209:213	arg1	thrips					259:264	the western flower thrips	240:264	the western flower thrips (WFT)	240:270	There is increasing evidence that acylsugars deter insect pests and plant virus vectors, including the western flower thrips (WFT), Frankliniella occidentalis (Pergande), vector of tomato spotted wilt virus (TSWV).
31748622	0	49	theme	virus	39:43	arg1	vector					9:14	A thrips vector	0:14	A thrips vector of tomato spotted wilt virus	0:43	A thrips vector of tomato spotted wilt virus responds to tomato acylsugar chemical diversity with reduced oviposition and virus inoculation.
31748622	8	50	theme	direct	1526:1531	arg1	pests					1533:1537	direct pests	1526:1537	direct pests	1526:1537	We show that increasing acylsucrose amount output by type IV trichomes and selecting for particular FA profiles through advanced breeding profoundly affects WFT behavior in ways that benefit management of WFT as direct pests and as TSWV vectors.
31748622	8	50	theme	direct	1526:1531	arg1	management					1505:1514	management	1505:1514	management of WFT	1505:1521	We show that increasing acylsucrose amount output by type IV trichomes and selecting for particular FA profiles through advanced breeding profoundly affects WFT behavior in ways that benefit management of WFT as direct pests and as TSWV vectors.
31748622	2	51	dep	glucose	503:509	arg1	moiety					550:555	moiety	550:555	moiety	550:555	Acylsugars are sugar-polyesters composed of saturated, un-saturated, and variously branched short and long chain fatty acids (FAs) esterified to a glucose (acylglucose) or sucrose (acylsucrose) moiety.
31748622	4	52	from	oviposition	773:783	arg1	lines					825:829	tomato lines	818:829	tomato lines bred to exude varying amounts of acylsucrose in association with diverse FA profiles	818:914	Towards this goal, we examined WFT oviposition and TSWV inoculation behavior on tomato lines bred to exude varying amounts of acylsucrose in association with diverse FA profiles.
31748622	0	53	theme	wilt	34:37	arg1	virus					39:43	tomato spotted wilt virus	19:43	tomato spotted wilt virus	19:43	A thrips vector of tomato spotted wilt virus responds to tomato acylsugar chemical diversity with reduced oviposition and virus inoculation.
31748622	4	54	theme	WFT	769:771	arg1	oviposition					773:783	WFT oviposition	769:783	WFT oviposition	769:783	Towards this goal, we examined WFT oviposition and TSWV inoculation behavior on tomato lines bred to exude varying amounts of acylsucrose in association with diverse FA profiles.
31748622	8	55	theme	TSWV	1546:1549	arg1	vectors					1551:1557	as TSWV vectors	1543:1557	as TSWV vectors	1543:1557	We show that increasing acylsucrose amount output by type IV trichomes and selecting for particular FA profiles through advanced breeding profoundly affects WFT behavior in ways that benefit management of WFT as direct pests and as TSWV vectors.
31748622	8	55	theme	TSWV	1546:1549	arg1	management					1505:1514	management	1505:1514	management of WFT	1505:1521	We show that increasing acylsucrose amount output by type IV trichomes and selecting for particular FA profiles through advanced breeding profoundly affects WFT behavior in ways that benefit management of WFT as direct pests and as TSWV vectors.
31748622	4	56	theme	diverse	896:902	arg1	profiles					907:914	diverse FA profiles	896:914	diverse FA profiles	896:914	Towards this goal, we examined WFT oviposition and TSWV inoculation behavior on tomato lines bred to exude varying amounts of acylsucrose in association with diverse FA profiles.
31748622	7	57	theme	acylsugar-mediated	1174:1191	arg1	resistance					1193:1202	improving acylsugar-mediated resistance	1164:1202	improving acylsugar-mediated resistance against WFT	1164:1214	These findings support improving acylsugar-mediated resistance against WFT by breeding tomatoes exuding greater amounts of acylsucrose associated with specific FA profiles.
31748622	5	58	theme	egg-laying	974:983	arg1	WFT					970:972	WFT egg-laying	970:983	WFT egg-laying (oviposition)	970:997	Our data show that as acylsucrose amounts increased, WFT egg-laying (oviposition) decreased and TSWV inoculation was suppressed.
31748622	5	58	theme	egg-laying	974:983	arg1	oviposition					986:996	oviposition	986:996	oviposition	986:996	Our data show that as acylsucrose amounts increased, WFT egg-laying (oviposition) decreased and TSWV inoculation was suppressed.
31748622	7	59	theme	FA	1301:1302	arg1	profiles					1304:1311	specific FA profiles	1292:1311	specific FA profiles	1292:1311	These findings support improving acylsugar-mediated resistance against WFT by breeding tomatoes exuding greater amounts of acylsucrose associated with specific FA profiles.
31748622	6	60	theme	flower	1054:1059	arg1	thrips					1061:1066	Western flower thrips	1046:1066	Western flower thrips	1046:1066	Western flower thrips also responded to FA profiles that included iC4, iC11, nC12 and nC10 FA.
31748622	1	61	theme	virus	215:219	arg1	vectors					221:227	plant virus vectors	209:227	plant virus vectors	209:227	There is increasing evidence that acylsugars deter insect pests and plant virus vectors, including the western flower thrips (WFT), Frankliniella occidentalis (Pergande), vector of tomato spotted wilt virus (TSWV).
31748622	1	61	theme	virus	215:219	arg1	thrips					259:264	the western flower thrips	240:264	the western flower thrips (WFT)	240:270	There is increasing evidence that acylsugars deter insect pests and plant virus vectors, including the western flower thrips (WFT), Frankliniella occidentalis (Pergande), vector of tomato spotted wilt virus (TSWV).
31748622	3	62	theme	acylsucrose	586:596	arg1	amount					598:603	acylsucrose amount	586:603	acylsucrose amount	586:603	We sought to understand how acylsucrose amount and composition of associated FA profiles interacted to mediate resistance to WFT oviposition and TSWV inoculation on tomato leaves.
31748622	3	62	theme	acylsucrose	586:596	arg1	profiles					638:645	associated FA profiles	624:645	associated FA profiles	624:645	We sought to understand how acylsucrose amount and composition of associated FA profiles interacted to mediate resistance to WFT oviposition and TSWV inoculation on tomato leaves.
31748622	6	63	theme	Western	1046:1052	arg1	thrips					1061:1066	Western flower thrips	1046:1066	Western flower thrips	1046:1066	Western flower thrips also responded to FA profiles that included iC4, iC11, nC12 and nC10 FA.
31748622	8	64	theme	as	1543:1544	arg1	vectors					1551:1557	as TSWV vectors	1543:1557	as TSWV vectors	1543:1557	We show that increasing acylsucrose amount output by type IV trichomes and selecting for particular FA profiles through advanced breeding profoundly affects WFT behavior in ways that benefit management of WFT as direct pests and as TSWV vectors.
31748622	8	64	theme	as	1543:1544	arg1	management					1505:1514	management	1505:1514	management of WFT	1505:1521	We show that increasing acylsucrose amount output by type IV trichomes and selecting for particular FA profiles through advanced breeding profoundly affects WFT behavior in ways that benefit management of WFT as direct pests and as TSWV vectors.
31748622	2	65	theme	chain	463:467	arg1	acids					475:479	saturated, un-saturated, and variously branched short and long chain fatty acids	400:479	saturated, un-saturated, and variously branched short and long chain fatty acids (FAs) esterified to a glucose (acylglucose) or sucrose (acylsucrose) moiety	400:555	Acylsugars are sugar-polyesters composed of saturated, un-saturated, and variously branched short and long chain fatty acids (FAs) esterified to a glucose (acylglucose) or sucrose (acylsucrose) moiety.
31748622	2	65	theme	chain	463:467	arg1	FAs					482:484	FAs	482:484	FAs	482:484	Acylsugars are sugar-polyesters composed of saturated, un-saturated, and variously branched short and long chain fatty acids (FAs) esterified to a glucose (acylglucose) or sucrose (acylsucrose) moiety.
31748622	7	66	theme	improving	1164:1172	arg1	resistance					1193:1202	improving acylsugar-mediated resistance	1164:1202	improving acylsugar-mediated resistance against WFT	1164:1214	These findings support improving acylsugar-mediated resistance against WFT by breeding tomatoes exuding greater amounts of acylsucrose associated with specific FA profiles.
31748622	2	67	theme	long	458:461	arg1	acids					475:479	saturated, un-saturated, and variously branched short and long chain fatty acids	400:479	saturated, un-saturated, and variously branched short and long chain fatty acids (FAs) esterified to a glucose (acylglucose) or sucrose (acylsucrose) moiety	400:555	Acylsugars are sugar-polyesters composed of saturated, un-saturated, and variously branched short and long chain fatty acids (FAs) esterified to a glucose (acylglucose) or sucrose (acylsucrose) moiety.
31748622	2	67	theme	long	458:461	arg1	FAs					482:484	FAs	482:484	FAs	482:484	Acylsugars are sugar-polyesters composed of saturated, un-saturated, and variously branched short and long chain fatty acids (FAs) esterified to a glucose (acylglucose) or sucrose (acylsucrose) moiety.
31748622	3	68	theme	FA	635:636	arg1	profiles					638:645	associated FA profiles	624:645	associated FA profiles	624:645	We sought to understand how acylsucrose amount and composition of associated FA profiles interacted to mediate resistance to WFT oviposition and TSWV inoculation on tomato leaves.
31748622	7	69	theme	specific	1292:1299	arg1	profiles					1304:1311	specific FA profiles	1292:1311	specific FA profiles	1292:1311	These findings support improving acylsugar-mediated resistance against WFT by breeding tomatoes exuding greater amounts of acylsucrose associated with specific FA profiles.
31748622	4	70	with	association	879:889	arg1	profiles					907:914	diverse FA profiles	896:914	diverse FA profiles	896:914	Towards this goal, we examined WFT oviposition and TSWV inoculation behavior on tomato lines bred to exude varying amounts of acylsucrose in association with diverse FA profiles.
31748622	2	71	theme	un-saturated	411:422	arg1	acids					475:479	saturated, un-saturated, and variously branched short and long chain fatty acids	400:479	saturated, un-saturated, and variously branched short and long chain fatty acids (FAs) esterified to a glucose (acylglucose) or sucrose (acylsucrose) moiety	400:555	Acylsugars are sugar-polyesters composed of saturated, un-saturated, and variously branched short and long chain fatty acids (FAs) esterified to a glucose (acylglucose) or sucrose (acylsucrose) moiety.
31748622	2	71	theme	un-saturated	411:422	arg1	FAs					482:484	FAs	482:484	FAs	482:484	Acylsugars are sugar-polyesters composed of saturated, un-saturated, and variously branched short and long chain fatty acids (FAs) esterified to a glucose (acylglucose) or sucrose (acylsucrose) moiety.
31748622	8	72	theme	amount	1350:1355	arg1	output					1357:1362	acylsucrose amount output	1338:1362	acylsucrose amount output by type IV trichomes	1338:1383	We show that increasing acylsucrose amount output by type IV trichomes and selecting for particular FA profiles through advanced breeding profoundly affects WFT behavior in ways that benefit management of WFT as direct pests and as TSWV vectors.
31560973	9	0	theme	carbohydrate-metabolizing	878:902	arg1	enzymes					904:910	carbohydrate-metabolizing enzymes	878:910	carbohydrate-metabolizing enzymes	878:910	dbCAN2 analyses revealed 91 genes related to carbohydrate-metabolizing enzymes.
31560973	6	1	theme	PAMC	426:429	arg1	28760					431:435	PAMC 28760	426:435	PAMC 28760 from Antarctic	426:450	PAMC 28760 from Antarctic lichen, and present the complete genome sequence with annotations describing its unique features.
31560973	7	2	theme	coding	599:604	arg1	sequences					610:618	4,278 coding DNA sequences	593:618	4,278 coding DNA sequences	593:618	The genome sequence has 58.85% GC content, 4,278 coding DNA sequences, 85 tRNAs, and 22 rRNA operons.
31560973	6	3	theme	unique	533:538	arg1	features					540:547	its unique features	529:547	its unique features	529:547	PAMC 28760 from Antarctic lichen, and present the complete genome sequence with annotations describing its unique features.
31560973	1	4	from	metabolism	135:144	arg1	Identification					55:68	Identification	55:68	Identification of CAZyme genes	55:84	PAMC 28760: Identification of CAZyme genes and analysis of their potential role in glycogen metabolism for cold survival adaptation.
31560973	1	4	from	metabolism	135:144	arg1	analysis					90:97	analysis	90:97	analysis of their potential role in glycogen metabolism for cold survival adaptation	90:173	PAMC 28760: Identification of CAZyme genes and analysis of their potential role in glycogen metabolism for cold survival adaptation.
31560973	8	5	theme	new	728:730	arg1	species					732:738	a potentially new species	714:738	a potentially new species of genus Shigella	714:756	16S rRNA gene sequence analyses revealed strain PAMC 28760 as a potentially new species of genus Shigella, showing various differences from pathogenic bacteria reported previously.
31560973	8	5	theme	new	728:730	arg1	28760					705:709	strain PAMC 28760	693:709	strain PAMC 28760	693:709	16S rRNA gene sequence analyses revealed strain PAMC 28760 as a potentially new species of genus Shigella, showing various differences from pathogenic bacteria reported previously.
31560973	1	6	from	role	118:121	arg1	metabolism					135:144	glycogen metabolism	126:144	glycogen metabolism	126:144	PAMC 28760: Identification of CAZyme genes and analysis of their potential role in glycogen metabolism for cold survival adaptation.
31560973	7	7	theme	58.85	574:578	arg1	%					579:579	%	579:579	%	579:579	The genome sequence has 58.85% GC content, 4,278 coding DNA sequences, 85 tRNAs, and 22 rRNA operons.
31560973	8	8	theme	pathogenic	792:801	arg1	bacteria					803:810	pathogenic bacteria	792:810	pathogenic bacteria reported previously	792:830	16S rRNA gene sequence analyses revealed strain PAMC 28760 as a potentially new species of genus Shigella, showing various differences from pathogenic bacteria reported previously.
31560973	8	9	theme	genus	743:747	arg1	Shigella					749:756	genus Shigella	743:756	genus Shigella	743:756	16S rRNA gene sequence analyses revealed strain PAMC 28760 as a potentially new species of genus Shigella, showing various differences from pathogenic bacteria reported previously.
31560973	8	10	from	bacteria	803:810	arg1	differences					775:785	various differences	767:785	various differences from pathogenic bacteria reported previously	767:830	16S rRNA gene sequence analyses revealed strain PAMC 28760 as a potentially new species of genus Shigella, showing various differences from pathogenic bacteria reported previously.
31560973	12	11	theme	adaptation	1081:1090	arg1	mechanisms					1092:1101	Shigella survival adaptation mechanisms	1063:1101	Shigella survival adaptation mechanisms	1063:1101	This study provides a foundation for understanding Shigella survival adaptation mechanisms under extremely cold Antarctic conditions.
31560973	7	12	theme	%	579:579	arg1	content					584:590	58.85% GC content	574:590	58.85% GC content	574:590	The genome sequence has 58.85% GC content, 4,278 coding DNA sequences, 85 tRNAs, and 22 rRNA operons.
31560973	4	13	theme	gram-negative	258:270	arg1	bacterium					289:297	a gram-negative, non-sporulating bacterium	256:297	bacterium	289:297	lichen in Antarctica) is a gram-negative, non-sporulating bacterium that has cellulolytic and amylolytic characteristics as well as glycogen metabolic pathways.
31560973	4	13	theme	gram-negative	258:270	arg1	lichen					231:236	lichen	231:236	lichen in Antarctica)	231:251	lichen in Antarctica) is a gram-negative, non-sporulating bacterium that has cellulolytic and amylolytic characteristics as well as glycogen metabolic pathways.
31560973	6	14	theme	genome	485:490	arg1	sequence					492:499	the complete genome sequence	472:499	the complete genome sequence	472:499	PAMC 28760 from Antarctic lichen, and present the complete genome sequence with annotations describing its unique features.
31560973	4	15	from	lichen	231:236	arg1	Antarctica					241:250	Antarctica	241:250	Antarctica	241:250	lichen in Antarctica) is a gram-negative, non-sporulating bacterium that has cellulolytic and amylolytic characteristics as well as glycogen metabolic pathways.
31560973	1	16	theme	cold	150:153	arg1	adaptation					164:173	cold survival adaptation	150:173	cold survival adaptation	150:173	PAMC 28760: Identification of CAZyme genes and analysis of their potential role in glycogen metabolism for cold survival adaptation.
31560973	6	17	theme	complete	476:483	arg1	sequence					492:499	the complete genome sequence	472:499	the complete genome sequence	472:499	PAMC 28760 from Antarctic lichen, and present the complete genome sequence with annotations describing its unique features.
31560973	7	18	theme	GC	581:582	arg1	content					584:590	58.85% GC content	574:590	58.85% GC content	574:590	The genome sequence has 58.85% GC content, 4,278 coding DNA sequences, 85 tRNAs, and 22 rRNA operons.
31560973	4	19	theme	non-sporulating	273:287	arg1	bacterium					289:297	a gram-negative, non-sporulating bacterium	256:297	bacterium	289:297	lichen in Antarctica) is a gram-negative, non-sporulating bacterium that has cellulolytic and amylolytic characteristics as well as glycogen metabolic pathways.
31560973	4	19	theme	non-sporulating	273:287	arg1	lichen					231:236	lichen	231:236	lichen in Antarctica)	231:251	lichen in Antarctica) is a gram-negative, non-sporulating bacterium that has cellulolytic and amylolytic characteristics as well as glycogen metabolic pathways.
31560973	1	20	theme	survival	155:162	arg1	adaptation					164:173	cold survival adaptation	150:173	cold survival adaptation	150:173	PAMC 28760: Identification of CAZyme genes and analysis of their potential role in glycogen metabolism for cold survival adaptation.
31560973	0	21	theme	genome	9:14	arg1	sequencing					16:25	Complete genome sequencing	0:25	Complete genome sequencing of Shigella sp	0:40	Complete genome sequencing of Shigella sp.
31560973	3	22	theme	Himantormia	215:225	arg1	sp					227:228	Himantormia sp	215:228	Himantormia sp	215:228	PAMC 28760 (isolated from Himantormia sp.
31560973	3	23	theme	PAMC	189:192	arg1	28760					194:198	PAMC 28760	189:198	PAMC 28760 (	189:200	PAMC 28760 (isolated from Himantormia sp.
31560973	1	24	theme	CAZyme	73:78	arg1	genes					80:84	CAZyme genes	73:84	CAZyme genes	73:84	PAMC 28760: Identification of CAZyme genes and analysis of their potential role in glycogen metabolism for cold survival adaptation.
31560973	7	25	theme	4,278	593:597	arg1	sequences					610:618	4,278 coding DNA sequences	593:618	4,278 coding DNA sequences	593:618	The genome sequence has 58.85% GC content, 4,278 coding DNA sequences, 85 tRNAs, and 22 rRNA operons.
31560973	0	26	theme	Complete	0:7	arg1	sequencing					16:25	Complete genome sequencing	0:25	Complete genome sequencing of Shigella sp	0:40	Complete genome sequencing of Shigella sp.
31560973	8	27	theme	rRNA	656:659	arg1	analyses					675:682	16S rRNA gene sequence analyses	652:682	16S rRNA gene sequence analyses	652:682	16S rRNA gene sequence analyses revealed strain PAMC 28760 as a potentially new species of genus Shigella, showing various differences from pathogenic bacteria reported previously.
31560973	4	28	theme	metabolic	372:380	arg1	pathways					382:389	glycogen metabolic pathways	363:389	cellulolytic and amylolytic characteristics as well as glycogen metabolic pathways	308:389	lichen in Antarctica) is a gram-negative, non-sporulating bacterium that has cellulolytic and amylolytic characteristics as well as glycogen metabolic pathways.
31560973	1	29	theme	genes	80:84	arg1	Identification					55:68	Identification	55:68	Identification of CAZyme genes	55:84	PAMC 28760: Identification of CAZyme genes and analysis of their potential role in glycogen metabolism for cold survival adaptation.
31560973	1	29	theme	genes	80:84	arg1	analysis					90:97	analysis	90:97	analysis of their potential role in glycogen metabolism for cold survival adaptation	90:173	PAMC 28760: Identification of CAZyme genes and analysis of their potential role in glycogen metabolism for cold survival adaptation.
31560973	12	30	theme	Shigella	1063:1070	arg1	mechanisms					1092:1101	Shigella survival adaptation mechanisms	1063:1101	Shigella survival adaptation mechanisms	1063:1101	This study provides a foundation for understanding Shigella survival adaptation mechanisms under extremely cold Antarctic conditions.
31560973	8	31	theme	16S	652:654	arg1	rRNA					656:659	16S rRNA	652:659	16S rRNA gene sequence analyses	652:682	16S rRNA gene sequence analyses revealed strain PAMC 28760 as a potentially new species of genus Shigella, showing various differences from pathogenic bacteria reported previously.
31560973	12	32	theme	survival	1072:1079	arg1	mechanisms					1092:1101	Shigella survival adaptation mechanisms	1063:1101	Shigella survival adaptation mechanisms	1063:1101	This study provides a foundation for understanding Shigella survival adaptation mechanisms under extremely cold Antarctic conditions.
31560973	4	33	theme	glycogen	363:370	arg1	pathways					382:389	glycogen metabolic pathways	363:389	cellulolytic and amylolytic characteristics as well as glycogen metabolic pathways	308:389	lichen in Antarctica) is a gram-negative, non-sporulating bacterium that has cellulolytic and amylolytic characteristics as well as glycogen metabolic pathways.
31560973	10	34	theme	S.	913:914	arg1	sp					916:917	S. sp	913:917	S. sp.	913:918	S. sp.
31560973	7	35	theme	genome	554:559	arg1	sequence					561:568	The genome sequence	550:568	The genome sequence	550:568	The genome sequence has 58.85% GC content, 4,278 coding DNA sequences, 85 tRNAs, and 22 rRNA operons.
31560973	8	36	theme	sequence	666:673	arg1	analyses					675:682	16S rRNA gene sequence analyses	652:682	16S rRNA gene sequence analyses	652:682	16S rRNA gene sequence analyses revealed strain PAMC 28760 as a potentially new species of genus Shigella, showing various differences from pathogenic bacteria reported previously.
31560973	11	37	theme	polysaccharide	947:960	arg1	starch					962:967	polysaccharide starch	947:967	polysaccharide starch	947:967	PAMC 28760 likely degrades polysaccharide starch to obtain glucose for energy conservation.
31560973	0	38	theme	sp	39:40	arg1	sequencing					16:25	Complete genome sequencing	0:25	Complete genome sequencing of Shigella sp	0:40	Complete genome sequencing of Shigella sp.
31560973	8	39	theme	gene	661:664	arg1	analyses					675:682	16S rRNA gene sequence analyses	652:682	16S rRNA gene sequence analyses	652:682	16S rRNA gene sequence analyses revealed strain PAMC 28760 as a potentially new species of genus Shigella, showing various differences from pathogenic bacteria reported previously.
31560973	0	40	theme	Shigella	30:37	arg1	sp					39:40	Shigella sp	30:40	Shigella sp	30:40	Complete genome sequencing of Shigella sp.
31560973	7	41	contain	has	570:572	arg2	sequences					610:618	4,278 coding DNA sequences	593:618	4,278 coding DNA sequences	593:618	The genome sequence has 58.85% GC content, 4,278 coding DNA sequences, 85 tRNAs, and 22 rRNA operons.
31560973	7	41	contain	has	570:572	arg2	operons					643:649	22 rRNA operons	635:649	22 rRNA operons	635:649	The genome sequence has 58.85% GC content, 4,278 coding DNA sequences, 85 tRNAs, and 22 rRNA operons.
31560973	7	41	contain	has	570:572	arg2	tRNAs					624:628	85 tRNAs	621:628	85 tRNAs	621:628	The genome sequence has 58.85% GC content, 4,278 coding DNA sequences, 85 tRNAs, and 22 rRNA operons.
31560973	7	41	contain	has	570:572	arg1	sequence					561:568	The genome sequence	550:568	The genome sequence	550:568	The genome sequence has 58.85% GC content, 4,278 coding DNA sequences, 85 tRNAs, and 22 rRNA operons.
31560973	7	41	contain	has	570:572	arg2	content					584:590	58.85% GC content	574:590	58.85% GC content	574:590	The genome sequence has 58.85% GC content, 4,278 coding DNA sequences, 85 tRNAs, and 22 rRNA operons.
31560973	9	42	theme	dbCAN2	833:838	arg1	analyses					840:847	dbCAN2 analyses	833:847	dbCAN2 analyses	833:847	dbCAN2 analyses revealed 91 genes related to carbohydrate-metabolizing enzymes.
31560973	4	43	theme	amylolytic	325:334	arg1	characteristics					336:350	cellulolytic and amylolytic characteristics	308:350	cellulolytic and amylolytic characteristics as well as glycogen metabolic pathways	308:389	lichen in Antarctica) is a gram-negative, non-sporulating bacterium that has cellulolytic and amylolytic characteristics as well as glycogen metabolic pathways.
31560973	8	44	theme	strain	693:698	arg1	species					732:738	a potentially new species	714:738	a potentially new species of genus Shigella	714:756	16S rRNA gene sequence analyses revealed strain PAMC 28760 as a potentially new species of genus Shigella, showing various differences from pathogenic bacteria reported previously.
31560973	8	44	theme	strain	693:698	arg1	28760					705:709	strain PAMC 28760	693:709	strain PAMC 28760	693:709	16S rRNA gene sequence analyses revealed strain PAMC 28760 as a potentially new species of genus Shigella, showing various differences from pathogenic bacteria reported previously.
31560973	12	45	theme	Antarctic	1124:1132	arg1	conditions					1134:1143	extremely cold Antarctic conditions	1109:1143	extremely cold Antarctic conditions	1109:1143	This study provides a foundation for understanding Shigella survival adaptation mechanisms under extremely cold Antarctic conditions.
31560973	11	46	theme	energy	991:996	arg1	conservation					998:1009	energy conservation	991:1009	energy conservation	991:1009	PAMC 28760 likely degrades polysaccharide starch to obtain glucose for energy conservation.
31560973	7	47	theme	rRNA	638:641	arg1	operons					643:649	22 rRNA operons	635:649	22 rRNA operons	635:649	The genome sequence has 58.85% GC content, 4,278 coding DNA sequences, 85 tRNAs, and 22 rRNA operons.
31560973	1	48	dep	Identification	55:68	arg1	PAMC					43:46	PAMC 28760	43:52	PAMC 28760	43:52	PAMC 28760: Identification of CAZyme genes and analysis of their potential role in glycogen metabolism for cold survival adaptation.
31560973	7	49	theme	DNA	606:608	arg1	sequences					610:618	4,278 coding DNA sequences	593:618	4,278 coding DNA sequences	593:618	The genome sequence has 58.85% GC content, 4,278 coding DNA sequences, 85 tRNAs, and 22 rRNA operons.
31560973	8	50	theme	PAMC	700:703	arg1	species					732:738	a potentially new species	714:738	a potentially new species of genus Shigella	714:756	16S rRNA gene sequence analyses revealed strain PAMC 28760 as a potentially new species of genus Shigella, showing various differences from pathogenic bacteria reported previously.
31560973	8	50	theme	PAMC	700:703	arg1	28760					705:709	strain PAMC 28760	693:709	strain PAMC 28760	693:709	16S rRNA gene sequence analyses revealed strain PAMC 28760 as a potentially new species of genus Shigella, showing various differences from pathogenic bacteria reported previously.
31560973	5	51	theme	S.	419:420	arg1	sp					422:423	S. sp	419:423	S. sp	419:423	In this study, we isolated S. sp.
31560973	6	52	from	Antarctic	442:450	arg1	28760					431:435	PAMC 28760	426:435	PAMC 28760 from Antarctic	426:450	PAMC 28760 from Antarctic lichen, and present the complete genome sequence with annotations describing its unique features.
31560973	12	53	theme	cold	1119:1122	arg1	conditions					1134:1143	extremely cold Antarctic conditions	1109:1143	extremely cold Antarctic conditions	1109:1143	This study provides a foundation for understanding Shigella survival adaptation mechanisms under extremely cold Antarctic conditions.
31560973	1	54	theme	potential	108:116	arg1	role					118:121	their potential role	102:121	their potential role in glycogen metabolism for cold survival adaptation	102:173	PAMC 28760: Identification of CAZyme genes and analysis of their potential role in glycogen metabolism for cold survival adaptation.
31560973	1	55	from	analysis	90:97	arg1	metabolism					135:144	glycogen metabolism	126:144	glycogen metabolism	126:144	PAMC 28760: Identification of CAZyme genes and analysis of their potential role in glycogen metabolism for cold survival adaptation.
31560973	9	56	theme	related	867:873	arg1	genes					861:865	91 genes	858:865	91 genes related to carbohydrate-metabolizing enzymes	858:910	dbCAN2 analyses revealed 91 genes related to carbohydrate-metabolizing enzymes.
31560973	1	57	theme	role	118:121	arg1	Identification					55:68	Identification	55:68	Identification of CAZyme genes	55:84	PAMC 28760: Identification of CAZyme genes and analysis of their potential role in glycogen metabolism for cold survival adaptation.
31560973	1	57	theme	role	118:121	arg1	analysis					90:97	analysis	90:97	analysis of their potential role in glycogen metabolism for cold survival adaptation	90:173	PAMC 28760: Identification of CAZyme genes and analysis of their potential role in glycogen metabolism for cold survival adaptation.
31560973	8	58	theme	various	767:773	arg1	differences					775:785	various differences	767:785	various differences from pathogenic bacteria reported previously	767:830	16S rRNA gene sequence analyses revealed strain PAMC 28760 as a potentially new species of genus Shigella, showing various differences from pathogenic bacteria reported previously.
31560973	4	59	contain	has	304:306	arg1	bacterium					289:297	a gram-negative, non-sporulating bacterium	256:297	bacterium	289:297	lichen in Antarctica) is a gram-negative, non-sporulating bacterium that has cellulolytic and amylolytic characteristics as well as glycogen metabolic pathways.
31560973	4	59	contain	has	304:306	arg2	characteristics					336:350	cellulolytic and amylolytic characteristics	308:350	cellulolytic and amylolytic characteristics as well as glycogen metabolic pathways	308:389	lichen in Antarctica) is a gram-negative, non-sporulating bacterium that has cellulolytic and amylolytic characteristics as well as glycogen metabolic pathways.
31560973	4	59	contain	has	304:306	arg2	pathways					382:389	glycogen metabolic pathways	363:389	cellulolytic and amylolytic characteristics as well as glycogen metabolic pathways	308:389	lichen in Antarctica) is a gram-negative, non-sporulating bacterium that has cellulolytic and amylolytic characteristics as well as glycogen metabolic pathways.
31560973	4	59	contain	has	304:306	arg1	lichen					231:236	lichen	231:236	lichen in Antarctica)	231:251	lichen in Antarctica) is a gram-negative, non-sporulating bacterium that has cellulolytic and amylolytic characteristics as well as glycogen metabolic pathways.
31560973	2	60	theme	Shigella	176:183	arg1	sp					185:186	Shigella sp	176:186	Shigella sp.	176:187	Shigella sp.
31560973	8	61	theme	Shigella	749:756	arg1	species					732:738	a potentially new species	714:738	a potentially new species of genus Shigella	714:756	16S rRNA gene sequence analyses revealed strain PAMC 28760 as a potentially new species of genus Shigella, showing various differences from pathogenic bacteria reported previously.
31560973	8	61	theme	Shigella	749:756	arg1	28760					705:709	strain PAMC 28760	693:709	strain PAMC 28760	693:709	16S rRNA gene sequence analyses revealed strain PAMC 28760 as a potentially new species of genus Shigella, showing various differences from pathogenic bacteria reported previously.
31560973	4	62	theme	cellulolytic	308:319	arg1	characteristics					336:350	cellulolytic and amylolytic characteristics	308:350	cellulolytic and amylolytic characteristics as well as glycogen metabolic pathways	308:389	lichen in Antarctica) is a gram-negative, non-sporulating bacterium that has cellulolytic and amylolytic characteristics as well as glycogen metabolic pathways.
31560973	1	63	theme	glycogen	126:133	arg1	metabolism					135:144	glycogen metabolism	126:144	glycogen metabolism	126:144	PAMC 28760: Identification of CAZyme genes and analysis of their potential role in glycogen metabolism for cold survival adaptation.
31560973	1	64	from	Identification	55:68	arg1	metabolism					135:144	glycogen metabolism	126:144	glycogen metabolism	126:144	PAMC 28760: Identification of CAZyme genes and analysis of their potential role in glycogen metabolism for cold survival adaptation.
29580476	4	0	theme	protein	961:967	arg1	ratio					979:983	The fat content, potassium content, specific surface, and the protein to lipids ratio	899:983	The fat content, potassium content, specific surface, and the protein to lipids ratio	899:983	The fat content, potassium content, specific surface, and the protein to lipids ratio correlated with the lag phase time, which is related with the seed coat impermeability to water.
29580476	1	1	theme	intrinsic	143:151	arg1	properties					153:162	the intrinsic properties	139:162	the intrinsic properties of beans	139:171	This work explained how the intrinsic properties of beans affects the hydration process.
29580476	6	2	theme	complex	1235:1241	arg1	phenomenon					1243:1252	a complex phenomenon	1233:1252	a complex phenomenon	1233:1252	It was concluded that the hydration of beans process is a complex phenomenon and that despite being from the same variety of legume, any change due to agronomic enhancement may affect their hydration process kinetics.
29580476	6	2	theme	complex	1235:1241	arg1	hydration					1203:1211	the hydration	1199:1211	the hydration of beans process	1199:1228	It was concluded that the hydration of beans process is a complex phenomenon and that despite being from the same variety of legume, any change due to agronomic enhancement may affect their hydration process kinetics.
29580476	2	3	dep	starch	517:522	arg1	lipids					534:539	lipids	534:539	lipids	534:539	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	2	3	dep	starch	517:522	arg1	protein					525:531	protein	525:531	protein	525:531	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	2	4	dep	properties	652:661	arg1	weight					681:686	1000 grain weight	670:686	1000 grain weight	670:686	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	2	4	dep	properties	652:661	arg1	energy					710:715	energy	710:715	energy to penetrate the bean	710:737	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	2	4	dep	properties	652:661	arg1	thickness					699:707	seed coat thickness	689:707	seed coat thickness	689:707	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	2	4	dep	properties	652:661	arg1	size					664:667	size	664:667	size	664:667	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	2	5	theme	coat	694:697	arg1	size					664:667	size	664:667	size	664:667	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	2	5	theme	coat	694:697	arg1	thickness					699:707	seed coat thickness	689:707	seed coat thickness	689:707	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	2	6	dep	Mg	552:553	arg1	P					556:556	P	556:556	P	556:556	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	2	6	dep	Mg	552:553	arg1	Zn					581:582	Zn	581:582	Zn	581:582	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	2	6	dep	Mg	552:553	arg1	S					559:559	S	559:559	S	559:559	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	2	6	dep	Mg	552:553	arg1	Mn					569:570	Mn	569:570	Mn	569:570	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	2	6	dep	Mg	552:553	arg1	Fe					573:574	Fe	573:574	Fe	573:574	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	2	6	dep	Mg	552:553	arg1	K					562:562	K	562:562	K	562:562	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	2	6	dep	Mg	552:553	arg1	Ca					565:566	Ca	565:566	Ca	565:566	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	2	6	dep	Mg	552:553	arg1	Cu					577:578	Cu	577:578	Cu	577:578	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	2	7	theme	physical	643:650	arg1	properties					652:661	physical properties	643:661	physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean)	643:738	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	2	8	theme	seed	689:692	arg1	size					664:667	size	664:667	size	664:667	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	2	8	theme	seed	689:692	arg1	thickness					699:707	seed coat thickness	689:707	seed coat thickness	689:707	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	6	9	theme	agronomic	1328:1336	arg1	enhancement					1338:1348	agronomic enhancement	1328:1348	agronomic enhancement	1328:1348	It was concluded that the hydration of beans process is a complex phenomenon and that despite being from the same variety of legume, any change due to agronomic enhancement may affect their hydration process kinetics.
29580476	2	10	dep	energy	710:715	arg1	penetrate					720:728	penetrate	720:728	to penetrate the bean	717:737	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	6	11	theme	legume	1302:1307	arg1	legume					1302:1307	legume	1302:1307	legume	1302:1307	It was concluded that the hydration of beans process is a complex phenomenon and that despite being from the same variety of legume, any change due to agronomic enhancement may affect their hydration process kinetics.
29580476	6	11	theme	legume	1302:1307	arg1	variety					1291:1297	the same variety	1282:1297	the same variety of legume	1282:1307	It was concluded that the hydration of beans process is a complex phenomenon and that despite being from the same variety of legume, any change due to agronomic enhancement may affect their hydration process kinetics.
29580476	3	12	theme	hydration	800:808	arg1	characteristics					819:833	the hydration kinetics characteristics	796:833	the hydration kinetics characteristics of the studied bean	796:853	Only few properties correlated with the hydration kinetics characteristics of the studied bean, comprising both composition and structure.
29580476	4	13	theme	content	926:932	arg1	ratio					979:983	The fat content, potassium content, specific surface, and the protein to lipids ratio	899:983	The fat content, potassium content, specific surface, and the protein to lipids ratio	899:983	The fat content, potassium content, specific surface, and the protein to lipids ratio correlated with the lag phase time, which is related with the seed coat impermeability to water.
29580476	4	14	theme	specific	935:942	arg1	surface					944:950	specific surface	935:950	specific surface	935:950	The fat content, potassium content, specific surface, and the protein to lipids ratio correlated with the lag phase time, which is related with the seed coat impermeability to water.
29580476	5	15	theme	seed	1120:1123	arg1	coat					1125:1128	the seed coat	1116:1128	the seed coat	1116:1128	The necessary energy to perforate the seed coat correlated negatively with the hydration rate.
29580476	3	16	theme	kinetics	810:817	arg1	characteristics					819:833	the hydration kinetics characteristics	796:833	the hydration kinetics characteristics of the studied bean	796:853	Only few properties correlated with the hydration kinetics characteristics of the studied bean, comprising both composition and structure.
29580476	2	17	theme	bean	263:266	arg1	cultivars					242:250	six cultivars	238:250	six cultivars of carioca bean (a variety of common bean)	238:293	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	0	18	theme	hydration	95:103	arg1	kinetics					105:112	their hydration kinetics	89:112	their hydration kinetics	89:112	Correlating the properties of different carioca bean cultivars (Phaseolus vulgaris) with their hydration kinetics.
29580476	3	19	theme	bean	850:853	arg1	characteristics					819:833	the hydration kinetics characteristics	796:833	the hydration kinetics characteristics of the studied bean	796:853	Only few properties correlated with the hydration kinetics characteristics of the studied bean, comprising both composition and structure.
29580476	2	20	theme	carioca	255:261	arg1	bean					263:266	carioca bean	255:266	carioca bean (a variety of common bean)	255:293	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	2	21	theme	functional	586:595	arg1	groups					597:602	functional groups	586:602	functional groups	586:602	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	2	21	theme	functional	586:595	arg1	lipids					534:539	lipids	534:539	lipids	534:539	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	1	22	theme	beans	167:171	arg1	properties					153:162	the intrinsic properties	139:162	the intrinsic properties of beans	139:171	This work explained how the intrinsic properties of beans affects the hydration process.
29580476	4	23	with	related	1030:1036	arg1	impermeability					1057:1070	the seed coat impermeability	1043:1070	the seed coat impermeability to water	1043:1079	The fat content, potassium content, specific surface, and the protein to lipids ratio correlated with the lag phase time, which is related with the seed coat impermeability to water.
29580476	2	24	theme	Multiple	456:463	arg1	Analysis					475:482	a Multiple Factorial Analysis	454:482	a Multiple Factorial Analysis (MFA)	454:488	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	2	24	theme	Multiple	456:463	arg1	MFA					485:487	MFA	485:487	MFA	485:487	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	2	25	theme	bean	289:292	arg1	variety					271:277	a variety	269:277	a variety of common bean	269:292	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	2	25	theme	bean	289:292	arg1	bean					289:292	common bean	282:292	common bean	282:292	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	4	26	theme	surface	944:950	arg1	ratio					979:983	The fat content, potassium content, specific surface, and the protein to lipids ratio	899:983	The fat content, potassium content, specific surface, and the protein to lipids ratio	899:983	The fat content, potassium content, specific surface, and the protein to lipids ratio correlated with the lag phase time, which is related with the seed coat impermeability to water.
29580476	2	27	theme	hydration	346:354	arg1	time					408:411	lag phase time	398:411	lag phase time	398:411	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	2	27	theme	hydration	346:354	arg1	content					438:444	equilibrium moisture content	417:444	equilibrium moisture content	417:444	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	2	27	theme	hydration	346:354	arg1	characteristics					365:379	their hydration kinetics characteristics	340:379	their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content)	340:445	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	2	27	theme	hydration	346:354	arg1	rate					392:395	hydration rate	382:395	hydration rate	382:395	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	2	28	dep	minerals	542:549	arg1	Mg					552:553	Mg	552:553	Mg	552:553	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	6	29	theme	same	1286:1289	arg1	legume					1302:1307	legume	1302:1307	legume	1302:1307	It was concluded that the hydration of beans process is a complex phenomenon and that despite being from the same variety of legume, any change due to agronomic enhancement may affect their hydration process kinetics.
29580476	6	29	theme	same	1286:1289	arg1	variety					1291:1297	the same variety	1282:1297	the same variety of legume	1282:1307	It was concluded that the hydration of beans process is a complex phenomenon and that despite being from the same variety of legume, any change due to agronomic enhancement may affect their hydration process kinetics.
29580476	6	30	theme	process	1377:1383	arg1	kinetics					1385:1392	their hydration process kinetics	1361:1392	their hydration process kinetics	1361:1392	It was concluded that the hydration of beans process is a complex phenomenon and that despite being from the same variety of legume, any change due to agronomic enhancement may affect their hydration process kinetics.
29580476	2	31	theme	common	282:287	arg1	bean					289:292	common bean	282:292	common bean	282:292	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	0	32	theme	bean	48:51	arg1	vulgaris					74:81	Phaseolus vulgaris	64:81	Phaseolus vulgaris	64:81	Correlating the properties of different carioca bean cultivars (Phaseolus vulgaris) with their hydration kinetics.
29580476	0	32	theme	bean	48:51	arg1	cultivars					53:61	different carioca bean cultivars	30:61	different carioca bean cultivars (Phaseolus vulgaris)	30:82	Correlating the properties of different carioca bean cultivars (Phaseolus vulgaris) with their hydration kinetics.
29580476	2	33	theme	phase	402:406	arg1	time					408:411	lag phase time	398:411	lag phase time	398:411	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	2	33	theme	phase	402:406	arg1	characteristics					365:379	their hydration kinetics characteristics	340:379	their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content)	340:445	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	2	34	theme	chemical	495:502	arg1	composition					504:514	the chemical composition	491:514	the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR)	491:640	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	3	35	theme	studied	842:848	arg1	bean					850:853	the studied bean	838:853	the studied bean	838:853	Only few properties correlated with the hydration kinetics characteristics of the studied bean, comprising both composition and structure.
29580476	4	36	theme	content	907:913	arg1	ratio					979:983	The fat content, potassium content, specific surface, and the protein to lipids ratio	899:983	The fat content, potassium content, specific surface, and the protein to lipids ratio	899:983	The fat content, potassium content, specific surface, and the protein to lipids ratio correlated with the lag phase time, which is related with the seed coat impermeability to water.
29580476	1	37	theme	hydration	185:193	arg1	process					195:201	the hydration process	181:201	the hydration process	181:201	This work explained how the intrinsic properties of beans affects the hydration process.
29580476	2	38	theme	lag	398:400	arg1	time					408:411	lag phase time	398:411	lag phase time	398:411	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	2	38	theme	lag	398:400	arg1	characteristics					365:379	their hydration kinetics characteristics	340:379	their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content)	340:445	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	2	39	dep	analyzed	300:307	arg1	properties					652:661	physical properties	643:661	physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean)	643:738	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	2	39	dep	analyzed	300:307	arg1	microstructure					744:757	microstructure	744:757	microstructure	744:757	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	2	39	dep	analyzed	300:307	arg1	composition					504:514	the chemical composition	491:514	the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR)	491:640	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	2	40	theme	seed	613:616	arg1	coat					618:621	the seed coat	609:621	the seed coat	609:621	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	0	41	theme	carioca	40:46	arg1	vulgaris					74:81	Phaseolus vulgaris	64:81	Phaseolus vulgaris	64:81	Correlating the properties of different carioca bean cultivars (Phaseolus vulgaris) with their hydration kinetics.
29580476	0	41	theme	carioca	40:46	arg1	cultivars					53:61	different carioca bean cultivars	30:61	different carioca bean cultivars (Phaseolus vulgaris)	30:82	Correlating the properties of different carioca bean cultivars (Phaseolus vulgaris) with their hydration kinetics.
29580476	4	42	theme	potassium	916:924	arg1	content					926:932	potassium content	916:932	potassium content	916:932	The fat content, potassium content, specific surface, and the protein to lipids ratio correlated with the lag phase time, which is related with the seed coat impermeability to water.
29580476	2	43	theme	hydration	382:390	arg1	characteristics					365:379	their hydration kinetics characteristics	340:379	their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content)	340:445	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	2	43	theme	hydration	382:390	arg1	rate					392:395	hydration rate	382:395	hydration rate	382:395	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	0	44	theme	different	30:38	arg1	vulgaris					74:81	Phaseolus vulgaris	64:81	Phaseolus vulgaris	64:81	Correlating the properties of different carioca bean cultivars (Phaseolus vulgaris) with their hydration kinetics.
29580476	0	44	theme	different	30:38	arg1	cultivars					53:61	different carioca bean cultivars	30:61	different carioca bean cultivars (Phaseolus vulgaris)	30:82	Correlating the properties of different carioca bean cultivars (Phaseolus vulgaris) with their hydration kinetics.
29580476	2	45	theme	different	214:222	arg1	properties					224:233	different properties	214:233	different properties of six cultivars of carioca bean (a variety of common bean)	214:293	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	5	46	theme	hydration	1161:1169	arg1	rate					1171:1174	the hydration rate	1157:1174	the hydration rate	1157:1174	The necessary energy to perforate the seed coat correlated negatively with the hydration rate.
29580476	2	47	theme	Factorial	465:473	arg1	Analysis					475:482	a Multiple Factorial Analysis	454:482	a Multiple Factorial Analysis (MFA)	454:488	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	2	47	theme	Factorial	465:473	arg1	MFA					485:487	MFA	485:487	MFA	485:487	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	0	48	theme	cultivars	53:61	arg1	properties					16:25	the properties	12:25	the properties of different carioca bean cultivars (Phaseolus vulgaris)	12:82	Correlating the properties of different carioca bean cultivars (Phaseolus vulgaris) with their hydration kinetics.
29580476	2	49	dep	composition	504:514	arg1	starch					517:522	starch	517:522	starch	517:522	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	3	50	theme	few	765:767	arg1	properties					769:778	Only few properties	760:778	Only few properties	760:778	Only few properties correlated with the hydration kinetics characteristics of the studied bean, comprising both composition and structure.
29580476	6	51	theme	beans	1216:1220	arg1	process					1222:1228	beans process	1216:1228	beans process	1216:1228	It was concluded that the hydration of beans process is a complex phenomenon and that despite being from the same variety of legume, any change due to agronomic enhancement may affect their hydration process kinetics.
29580476	2	52	theme	equilibrium	417:427	arg1	characteristics					365:379	their hydration kinetics characteristics	340:379	their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content)	340:445	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	2	52	theme	equilibrium	417:427	arg1	content					438:444	equilibrium moisture content	417:444	equilibrium moisture content	417:444	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	6	53	theme	hydration	1367:1375	arg1	kinetics					1385:1392	their hydration process kinetics	1361:1392	their hydration process kinetics	1361:1392	It was concluded that the hydration of beans process is a complex phenomenon and that despite being from the same variety of legume, any change due to agronomic enhancement may affect their hydration process kinetics.
29580476	4	54	theme	phase	1009:1013	arg1	time					1015:1018	the lag phase time	1001:1018	the lag phase time	1001:1018	The fat content, potassium content, specific surface, and the protein to lipids ratio correlated with the lag phase time, which is related with the seed coat impermeability to water.
29580476	4	54	theme	phase	1009:1013	arg1	related					1030:1036	related	1030:1036	related	1030:1036	The fat content, potassium content, specific surface, and the protein to lipids ratio correlated with the lag phase time, which is related with the seed coat impermeability to water.
29580476	4	55	theme	lag	1005:1007	arg1	time					1015:1018	the lag phase time	1001:1018	the lag phase time	1001:1018	The fat content, potassium content, specific surface, and the protein to lipids ratio correlated with the lag phase time, which is related with the seed coat impermeability to water.
29580476	4	55	theme	lag	1005:1007	arg1	related					1030:1036	related	1030:1036	related	1030:1036	The fat content, potassium content, specific surface, and the protein to lipids ratio correlated with the lag phase time, which is related with the seed coat impermeability to water.
29580476	4	56	theme	coat	1052:1055	arg1	impermeability					1057:1070	the seed coat impermeability	1043:1070	the seed coat impermeability to water	1043:1079	The fat content, potassium content, specific surface, and the protein to lipids ratio correlated with the lag phase time, which is related with the seed coat impermeability to water.
29580476	2	57	theme	cultivars	242:250	arg1	properties					224:233	different properties	214:233	different properties of six cultivars of carioca bean (a variety of common bean)	214:293	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	5	58	dep	energy	1096:1101	arg1	perforate					1106:1114	perforate	1106:1114	to perforate the seed coat	1103:1128	The necessary energy to perforate the seed coat correlated negatively with the hydration rate.
29580476	5	59	theme	necessary	1086:1094	arg1	energy					1096:1101	The necessary energy	1082:1101	The necessary energy to perforate the seed coat	1082:1128	The necessary energy to perforate the seed coat correlated negatively with the hydration rate.
29580476	4	60	theme	fat	903:905	arg1	content					907:913	fat content	903:913	fat content	903:913	The fat content, potassium content, specific surface, and the protein to lipids ratio correlated with the lag phase time, which is related with the seed coat impermeability to water.
29580476	2	61	theme	grain	675:679	arg1	weight					681:686	1000 grain weight	670:686	1000 grain weight	670:686	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	2	61	theme	grain	675:679	arg1	size					664:667	size	664:667	size	664:667	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	0	62	theme	Phaseolus	64:72	arg1	vulgaris					74:81	Phaseolus vulgaris	64:81	Phaseolus vulgaris	64:81	Correlating the properties of different carioca bean cultivars (Phaseolus vulgaris) with their hydration kinetics.
29580476	0	62	theme	Phaseolus	64:72	arg1	cultivars					53:61	different carioca bean cultivars	30:61	different carioca bean cultivars (Phaseolus vulgaris)	30:82	Correlating the properties of different carioca bean cultivars (Phaseolus vulgaris) with their hydration kinetics.
29580476	4	63	theme	seed	1047:1050	arg1	impermeability					1057:1070	the seed coat impermeability	1043:1070	the seed coat impermeability to water	1043:1079	The fat content, potassium content, specific surface, and the protein to lipids ratio correlated with the lag phase time, which is related with the seed coat impermeability to water.
29580476	2	64	dep	characteristics	365:379	arg1	time					408:411	lag phase time	398:411	lag phase time	398:411	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	2	64	dep	characteristics	365:379	arg1	content					438:444	equilibrium moisture content	417:444	equilibrium moisture content	417:444	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	2	64	dep	characteristics	365:379	arg1	characteristics					365:379	their hydration kinetics characteristics	340:379	their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content)	340:445	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	2	64	dep	characteristics	365:379	arg1	rate					392:395	hydration rate	382:395	hydration rate	382:395	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	2	65	dep	bean	263:266	arg1	variety					271:277	a variety	269:277	a variety of common bean	269:292	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	2	65	dep	bean	263:266	arg1	bean					289:292	common bean	282:292	common bean	282:292	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	2	66	theme	kinetics	356:363	arg1	time					408:411	lag phase time	398:411	lag phase time	398:411	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	2	66	theme	kinetics	356:363	arg1	content					438:444	equilibrium moisture content	417:444	equilibrium moisture content	417:444	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	2	66	theme	kinetics	356:363	arg1	characteristics					365:379	their hydration kinetics characteristics	340:379	their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content)	340:445	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	2	66	theme	kinetics	356:363	arg1	rate					392:395	hydration rate	382:395	hydration rate	382:395	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	2	67	theme	moisture	429:436	arg1	characteristics					365:379	their hydration kinetics characteristics	340:379	their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content)	340:445	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	2	67	theme	moisture	429:436	arg1	content					438:444	equilibrium moisture content	417:444	equilibrium moisture content	417:444	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	2	68	from	coat	618:621	arg1	lipids					534:539	lipids	534:539	lipids	534:539	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	2	68	from	coat	618:621	arg1	protein					525:531	protein	525:531	protein	525:531	For that, different properties of six cultivars of carioca bean (a variety of common bean) were analyzed to verify the correlation with their hydration kinetics characteristics (hydration rate, lag phase time and equilibrium moisture content), using a Multiple Factorial Analysis (MFA): the chemical composition (starch, protein, lipids, minerals (Mg, P, S, K, Ca, Mn, Fe, Cu, Zn), functional groups from the seed coat analyzed by FT-IR), physical properties (size, 1000 grain weight, seed coat thickness, energy to penetrate the bean) and microstructure.
29580476	6	69	theme	process	1222:1228	arg1	phenomenon					1243:1252	a complex phenomenon	1233:1252	a complex phenomenon	1233:1252	It was concluded that the hydration of beans process is a complex phenomenon and that despite being from the same variety of legume, any change due to agronomic enhancement may affect their hydration process kinetics.
29580476	6	69	theme	process	1222:1228	arg1	hydration					1203:1211	the hydration	1199:1211	the hydration of beans process	1199:1228	It was concluded that the hydration of beans process is a complex phenomenon and that despite being from the same variety of legume, any change due to agronomic enhancement may affect their hydration process kinetics.
30572803	11	0	theme	primary	1988:1994	arg1	hHEP					1996:1999	primary hHEP	1988:1999	primary hHEP	1988:1999	The GAG profile of primary hHEP showed an HS-rich ECM, and thus, it may be possible to guide hBMSC differentiation to more mature hepatocytes by controlling the GAG profile expressed by differentiating cells.
30572803	6	1	theme	culture	1025:1031	arg1	conditions/protocols					1033:1052	four distinct culture conditions/protocols	1011:1052	four distinct culture conditions/protocols (T1-T4)	1011:1060	In this study, we examine the influence of four distinct culture conditions/protocols (T1-T4) on GAG composition and hepatic markers.
30572803	6	1	theme	culture	1025:1031	arg1	T1-T4					1055:1059	T1-T4	1055:1059	T1-T4	1055:1059	In this study, we examine the influence of four distinct culture conditions/protocols (T1-T4) on GAG composition and hepatic markers.
30572803	8	2	theme	unique	1539:1544	arg1	complex					1546:1552	a unique complex	1537:1552	a unique complex that diversifies the biological functions of HGF	1537:1601	Hepatocyte growth factor (HGF) appears to be more effective than activin A in promoting hepatic-like cells through the mesenchymal-epithelial transition, perhaps due to the former binding to the HGF receptor to form a unique complex that diversifies the biological functions of HGF.
30572803	6	3	from	influence	998:1006	arg1	composition					1069:1079	GAG composition	1065:1079	GAG composition	1065:1079	In this study, we examine the influence of four distinct culture conditions/protocols (T1-T4) on GAG composition and hepatic markers.
30572803	6	3	from	influence	998:1006	arg1	markers					1093:1099	hepatic markers	1085:1099	hepatic markers	1085:1099	In this study, we examine the influence of four distinct culture conditions/protocols (T1-T4) on GAG composition and hepatic markers.
30572803	2	4	theme	vast	337:340	arg1	majority					342:349	the vast majority	333:349	the vast majority of primary hHEP	333:365	Currently, the vast majority of primary hHEP are obtained from human tissue through cadavers.
30572803	11	5	theme	HS-rich	2011:2017	arg1	ECM					2019:2021	an HS-rich ECM	2008:2021	an HS-rich ECM	2008:2021	The GAG profile of primary hHEP showed an HS-rich ECM, and thus, it may be possible to guide hBMSC differentiation to more mature hepatocytes by controlling the GAG profile expressed by differentiating cells.
30572803	9	6	theme	growth	1818:1823	arg1	factors					1825:1831	growth factors	1818:1831	growth factors	1818:1831	Of the four protocols tested, uniform hepatocyte-like morphological changes, ALB secretion, and glycogen storage were found to be highest with protocol T2, which involves both early- and late-stage combinations of growth factors.
30572803	4	7	theme	bone	636:639	arg1	marrow					641:646	human bone marrow	630:646	human bone marrow mesenchymal stromal cells (hBMSCs)	630:681	With respect to the latter, human bone marrow mesenchymal stromal cells (hBMSCs) can serve as a source of autogenetic and allogenic multipotent stem cells for liver repair and regeneration.
30572803	10	8	theme	total	1838:1842	arg1	rich					1876:1879	rich	1876:1879	rich	1876:1879	The total GAG profile of the hBMSC ECM is rich in heparan sulfate (HS) and hyaluronan, both of which fluctuate during differentiation.
30572803	10	8	theme	total	1838:1842	arg1	profile					1848:1854	The total GAG profile	1834:1854	The total GAG profile of the hBMSC ECM	1834:1871	The total GAG profile of the hBMSC ECM is rich in heparan sulfate (HS) and hyaluronan, both of which fluctuate during differentiation.
30572803	11	9	theme	GAG	1973:1975	arg1	profile					1977:1983	The GAG profile	1969:1983	The GAG profile of primary hHEP	1969:1999	The GAG profile of primary hHEP showed an HS-rich ECM, and thus, it may be possible to guide hBMSC differentiation to more mature hepatocytes by controlling the GAG profile expressed by differentiating cells.
30572803	4	10	theme	mesenchymal	648:658	arg1	hBMSCs					675:680	hBMSCs	675:680	hBMSCs	675:680	With respect to the latter, human bone marrow mesenchymal stromal cells (hBMSCs) can serve as a source of autogenetic and allogenic multipotent stem cells for liver repair and regeneration.
30572803	4	10	theme	mesenchymal	648:658	arg1	cells					668:672	human bone marrow mesenchymal stromal cells	630:672	human bone marrow mesenchymal stromal cells (hBMSCs)	630:681	With respect to the latter, human bone marrow mesenchymal stromal cells (hBMSCs) can serve as a source of autogenetic and allogenic multipotent stem cells for liver repair and regeneration.
30572803	4	10	theme	mesenchymal	648:658	arg1	source					698:703	a source	696:703	a source of autogenetic and allogenic multipotent stem cells for liver repair and regeneration	696:789	With respect to the latter, human bone marrow mesenchymal stromal cells (hBMSCs) can serve as a source of autogenetic and allogenic multipotent stem cells for liver repair and regeneration.
30572803	10	11	theme	ECM	1869:1871	arg1	rich					1876:1879	rich	1876:1879	rich	1876:1879	The total GAG profile of the hBMSC ECM is rich in heparan sulfate (HS) and hyaluronan, both of which fluctuate during differentiation.
30572803	10	11	theme	ECM	1869:1871	arg1	profile					1848:1854	The total GAG profile	1834:1854	The total GAG profile of the hBMSC ECM	1834:1871	The total GAG profile of the hBMSC ECM is rich in heparan sulfate (HS) and hyaluronan, both of which fluctuate during differentiation.
30572803	1	12	theme	liver	192:196	arg1	repair					198:203	liver repair	192:203	liver repair	192:203	There is a critical need to generate functional hepatocytes to aid in liver repair and regeneration upon availability of a renewable, and potentially personalized, source of human hepatocytes (hHEP).
30572803	11	13	theme	differentiating	2155:2169	arg1	cells					2171:2175	differentiating cells	2155:2175	differentiating cells	2155:2175	The GAG profile of primary hHEP showed an HS-rich ECM, and thus, it may be possible to guide hBMSC differentiation to more mature hepatocytes by controlling the GAG profile expressed by differentiating cells.
30572803	6	14	theme	distinct	1016:1023	arg1	conditions/protocols					1033:1052	four distinct culture conditions/protocols	1011:1052	four distinct culture conditions/protocols (T1-T4)	1011:1060	In this study, we examine the influence of four distinct culture conditions/protocols (T1-T4) on GAG composition and hepatic markers.
30572803	6	14	theme	distinct	1016:1023	arg1	T1-T4					1055:1059	T1-T4	1055:1059	T1-T4	1055:1059	In this study, we examine the influence of four distinct culture conditions/protocols (T1-T4) on GAG composition and hepatic markers.
30572803	1	15	dep	renewable	245:253	arg1	personalized					272:283	personalized	272:283	personalized	272:283	There is a critical need to generate functional hepatocytes to aid in liver repair and regeneration upon availability of a renewable, and potentially personalized, source of human hepatocytes (hHEP).
30572803	2	16	theme	hHEP	362:365	arg1	majority					342:349	the vast majority	333:349	the vast majority of primary hHEP	333:365	Currently, the vast majority of primary hHEP are obtained from human tissue through cadavers.
30572803	10	17	theme	heparan	1884:1890	arg1	HS					1901:1902	HS	1901:1902	HS	1901:1902	The total GAG profile of the hBMSC ECM is rich in heparan sulfate (HS) and hyaluronan, both of which fluctuate during differentiation.
30572803	10	17	theme	heparan	1884:1890	arg1	sulfate					1892:1898	heparan sulfate	1884:1898	heparan sulfate (HS)	1884:1903	The total GAG profile of the hBMSC ECM is rich in heparan sulfate (HS) and hyaluronan, both of which fluctuate during differentiation.
30572803	3	18	theme	stem	590:593	arg1	cells					595:599	adult stem cells	584:599	adult stem cells	584:599	Recent advances in stem cell differentiation have opened up the possibility to obtain fully functional hepatocytes from embryonic or induced pluripotent stem cells, or adult stem cells.
30572803	5	19	theme	hBMSC	810:814	arg1	differentiation					816:830	hBMSC differentiation	810:830	hBMSC differentiation	810:830	A major aspect of hBMSC differentiation is the extracellular matrix (ECM) composition and, in particular, the role of glycosaminoglycans (GAGs) in the differentiation process.
30572803	9	20	theme	hepatocyte-like	1642:1656	arg1	changes					1672:1678	uniform hepatocyte-like morphological changes	1634:1678	uniform hepatocyte-like morphological changes	1634:1678	Of the four protocols tested, uniform hepatocyte-like morphological changes, ALB secretion, and glycogen storage were found to be highest with protocol T2, which involves both early- and late-stage combinations of growth factors.
30572803	11	21	theme	hBMSC	2062:2066	arg1	differentiation					2068:2082	hBMSC differentiation	2062:2082	hBMSC differentiation	2062:2082	The GAG profile of primary hHEP showed an HS-rich ECM, and thus, it may be possible to guide hBMSC differentiation to more mature hepatocytes by controlling the GAG profile expressed by differentiating cells.
30572803	3	22	from	cells	574:578	arg1	hepatocytes					519:529	fully functional hepatocytes	502:529	fully functional hepatocytes from embryonic or induced pluripotent stem cells, or adult stem cells	502:599	Recent advances in stem cell differentiation have opened up the possibility to obtain fully functional hepatocytes from embryonic or induced pluripotent stem cells, or adult stem cells.
30572803	0	23	theme	Bone	71:74	arg1	Cells					96:100	Adult Human Bone Mesenchymal Stromal Cells	59:100	Adult Human Bone Mesenchymal Stromal Cells	59:100	Remodeling of Glycosaminoglycans During Differentiation of Adult Human Bone Mesenchymal Stromal Cells Toward Hepatocytes.
30572803	9	24	theme	ALB	1681:1683	arg1	secretion					1685:1693	ALB secretion	1681:1693	ALB secretion	1681:1693	Of the four protocols tested, uniform hepatocyte-like morphological changes, ALB secretion, and glycogen storage were found to be highest with protocol T2, which involves both early- and late-stage combinations of growth factors.
30572803	0	25	theme	Stromal	88:94	arg1	Cells					96:100	Adult Human Bone Mesenchymal Stromal Cells	59:100	Adult Human Bone Mesenchymal Stromal Cells	59:100	Remodeling of Glycosaminoglycans During Differentiation of Adult Human Bone Mesenchymal Stromal Cells Toward Hepatocytes.
30572803	11	26	theme	mature	2092:2097	arg1	hepatocytes					2099:2109	more mature hepatocytes	2087:2109	more mature hepatocytes	2087:2109	The GAG profile of primary hHEP showed an HS-rich ECM, and thus, it may be possible to guide hBMSC differentiation to more mature hepatocytes by controlling the GAG profile expressed by differentiating cells.
30572803	4	27	theme	allogenic	724:732	arg1	cells					751:755	autogenetic and allogenic multipotent stem cells	708:755	autogenetic and allogenic multipotent stem cells	708:755	With respect to the latter, human bone marrow mesenchymal stromal cells (hBMSCs) can serve as a source of autogenetic and allogenic multipotent stem cells for liver repair and regeneration.
30572803	5	28	theme	glycosaminoglycans	910:927	arg1	role					902:905	the role	898:905	the role of glycosaminoglycans (GAGs) in the differentiation process	898:965	A major aspect of hBMSC differentiation is the extracellular matrix (ECM) composition and, in particular, the role of glycosaminoglycans (GAGs) in the differentiation process.
30572803	5	28	theme	glycosaminoglycans	910:927	arg1	aspect					800:805	A major aspect	792:805	A major aspect of hBMSC differentiation	792:830	A major aspect of hBMSC differentiation is the extracellular matrix (ECM) composition and, in particular, the role of glycosaminoglycans (GAGs) in the differentiation process.
30572803	5	28	theme	glycosaminoglycans	910:927	arg1	composition					866:876	the extracellular matrix (ECM) composition	835:876	the extracellular matrix (ECM) composition	835:876	A major aspect of hBMSC differentiation is the extracellular matrix (ECM) composition and, in particular, the role of glycosaminoglycans (GAGs) in the differentiation process.
30572803	9	29	theme	glycogen	1700:1707	arg1	storage					1709:1715	glycogen storage	1700:1715	glycogen storage	1700:1715	Of the four protocols tested, uniform hepatocyte-like morphological changes, ALB secretion, and glycogen storage were found to be highest with protocol T2, which involves both early- and late-stage combinations of growth factors.
30572803	4	30	theme	stem	746:749	arg1	cells					751:755	autogenetic and allogenic multipotent stem cells	708:755	autogenetic and allogenic multipotent stem cells	708:755	With respect to the latter, human bone marrow mesenchymal stromal cells (hBMSCs) can serve as a source of autogenetic and allogenic multipotent stem cells for liver repair and regeneration.
30572803	10	31	theme	GAG	1844:1846	arg1	rich					1876:1879	rich	1876:1879	rich	1876:1879	The total GAG profile of the hBMSC ECM is rich in heparan sulfate (HS) and hyaluronan, both of which fluctuate during differentiation.
30572803	10	31	theme	GAG	1844:1846	arg1	profile					1848:1854	The total GAG profile	1834:1854	The total GAG profile of the hBMSC ECM	1834:1871	The total GAG profile of the hBMSC ECM is rich in heparan sulfate (HS) and hyaluronan, both of which fluctuate during differentiation.
30572803	7	32	theme	hepatocyte	1120:1129	arg1	factor-4α					1139:1147	hepatocyte nuclear factor-4α	1120:1147	hepatocyte nuclear factor-4α	1120:1147	α-Fetoprotein and hepatocyte nuclear factor-4α were expressed continually over 21 days of differentiation, as indicated by real time quantitative PCR analysis, while albumin (ALB) expression did not begin until day 21.
30572803	5	33	from	composition	866:876	arg1	process					959:965	the differentiation process	939:965	the differentiation process	939:965	A major aspect of hBMSC differentiation is the extracellular matrix (ECM) composition and, in particular, the role of glycosaminoglycans (GAGs) in the differentiation process.
30572803	7	34	theme	albumin	1268:1274	arg1	expression					1282:1291	albumin (ALB) expression	1268:1291	albumin (ALB) expression	1268:1291	α-Fetoprotein and hepatocyte nuclear factor-4α were expressed continually over 21 days of differentiation, as indicated by real time quantitative PCR analysis, while albumin (ALB) expression did not begin until day 21.
30572803	4	35	theme	autogenetic	708:718	arg1	cells					751:755	autogenetic and allogenic multipotent stem cells	708:755	autogenetic and allogenic multipotent stem cells	708:755	With respect to the latter, human bone marrow mesenchymal stromal cells (hBMSCs) can serve as a source of autogenetic and allogenic multipotent stem cells for liver repair and regeneration.
30572803	0	36	theme	Glycosaminoglycans	14:31	arg1	Remodeling					0:9	Remodeling	0:9	Remodeling of Glycosaminoglycans During Differentiation of Adult Human Bone Mesenchymal Stromal Cells Toward Hepatocytes	0:119	Remodeling of Glycosaminoglycans During Differentiation of Adult Human Bone Mesenchymal Stromal Cells Toward Hepatocytes.
30572803	10	37	from	sulfate	1892:1898	arg1	rich					1876:1879	rich	1876:1879	rich	1876:1879	The total GAG profile of the hBMSC ECM is rich in heparan sulfate (HS) and hyaluronan, both of which fluctuate during differentiation.
30572803	10	37	from	sulfate	1892:1898	arg1	profile					1848:1854	The total GAG profile	1834:1854	The total GAG profile of the hBMSC ECM	1834:1871	The total GAG profile of the hBMSC ECM is rich in heparan sulfate (HS) and hyaluronan, both of which fluctuate during differentiation.
30572803	11	38	theme	GAG	2130:2132	arg1	profile					2134:2140	the GAG profile	2126:2140	the GAG profile expressed by differentiating cells	2126:2175	The GAG profile of primary hHEP showed an HS-rich ECM, and thus, it may be possible to guide hBMSC differentiation to more mature hepatocytes by controlling the GAG profile expressed by differentiating cells.
30572803	8	39	theme	Hepatocyte	1321:1330	arg1	HGF					1347:1349	HGF	1347:1349	HGF	1347:1349	Hepatocyte growth factor (HGF) appears to be more effective than activin A in promoting hepatic-like cells through the mesenchymal-epithelial transition, perhaps due to the former binding to the HGF receptor to form a unique complex that diversifies the biological functions of HGF.
30572803	8	39	theme	Hepatocyte	1321:1330	arg1	factor					1339:1344	Hepatocyte growth factor	1321:1344	Hepatocyte growth factor (HGF)	1321:1350	Hepatocyte growth factor (HGF) appears to be more effective than activin A in promoting hepatic-like cells through the mesenchymal-epithelial transition, perhaps due to the former binding to the HGF receptor to form a unique complex that diversifies the biological functions of HGF.
30572803	3	40	theme	stem	435:438	arg1	differentiation					445:459	stem cell differentiation	435:459	stem cell differentiation	435:459	Recent advances in stem cell differentiation have opened up the possibility to obtain fully functional hepatocytes from embryonic or induced pluripotent stem cells, or adult stem cells.
30572803	1	41	theme	hepatocytes	302:312	arg1	source					286:291	a renewable, and potentially personalized, source	243:291	a renewable, and potentially personalized, source of human hepatocytes (hHEP)	243:319	There is a critical need to generate functional hepatocytes to aid in liver repair and regeneration upon availability of a renewable, and potentially personalized, source of human hepatocytes (hHEP).
30572803	4	42	dep	latter	622:627	arg1	respect					607:613	respect	607:613	respect	607:613	With respect to the latter, human bone marrow mesenchymal stromal cells (hBMSCs) can serve as a source of autogenetic and allogenic multipotent stem cells for liver repair and regeneration.
30572803	7	43	theme	PCR	1248:1250	arg1	analysis					1252:1259	real time quantitative PCR analysis	1225:1259	real time quantitative PCR analysis	1225:1259	α-Fetoprotein and hepatocyte nuclear factor-4α were expressed continually over 21 days of differentiation, as indicated by real time quantitative PCR analysis, while albumin (ALB) expression did not begin until day 21.
30572803	9	44	theme	protocol	1747:1754	arg1	T2					1756:1757	protocol T2	1747:1757	protocol T2	1747:1757	Of the four protocols tested, uniform hepatocyte-like morphological changes, ALB secretion, and glycogen storage were found to be highest with protocol T2, which involves both early- and late-stage combinations of growth factors.
30572803	8	45	theme	former	1494:1499	arg1	binding					1501:1507	the former binding	1490:1507	the former binding to the HGF receptor	1490:1527	Hepatocyte growth factor (HGF) appears to be more effective than activin A in promoting hepatic-like cells through the mesenchymal-epithelial transition, perhaps due to the former binding to the HGF receptor to form a unique complex that diversifies the biological functions of HGF.
30572803	0	46	theme	Adult	59:63	arg1	Cells					96:100	Adult Human Bone Mesenchymal Stromal Cells	59:100	Adult Human Bone Mesenchymal Stromal Cells	59:100	Remodeling of Glycosaminoglycans During Differentiation of Adult Human Bone Mesenchymal Stromal Cells Toward Hepatocytes.
30572803	7	47	theme	time	1230:1233	arg1	analysis					1252:1259	real time quantitative PCR analysis	1225:1259	real time quantitative PCR analysis	1225:1259	α-Fetoprotein and hepatocyte nuclear factor-4α were expressed continually over 21 days of differentiation, as indicated by real time quantitative PCR analysis, while albumin (ALB) expression did not begin until day 21.
30572803	5	48	from	role	902:905	arg1	process					959:965	the differentiation process	939:965	the differentiation process	939:965	A major aspect of hBMSC differentiation is the extracellular matrix (ECM) composition and, in particular, the role of glycosaminoglycans (GAGs) in the differentiation process.
30572803	8	49	theme	HGF	1516:1518	arg1	receptor					1520:1527	the HGF receptor	1512:1527	the HGF receptor	1512:1527	Hepatocyte growth factor (HGF) appears to be more effective than activin A in promoting hepatic-like cells through the mesenchymal-epithelial transition, perhaps due to the former binding to the HGF receptor to form a unique complex that diversifies the biological functions of HGF.
30572803	3	50	theme	cell	440:443	arg1	differentiation					445:459	stem cell differentiation	435:459	stem cell differentiation	435:459	Recent advances in stem cell differentiation have opened up the possibility to obtain fully functional hepatocytes from embryonic or induced pluripotent stem cells, or adult stem cells.
30572803	2	51	theme	primary	354:360	arg1	hHEP					362:365	primary hHEP	354:365	primary hHEP	354:365	Currently, the vast majority of primary hHEP are obtained from human tissue through cadavers.
30572803	8	52	theme	biological	1575:1584	arg1	functions					1586:1594	the biological functions	1571:1594	the biological functions of HGF	1571:1601	Hepatocyte growth factor (HGF) appears to be more effective than activin A in promoting hepatic-like cells through the mesenchymal-epithelial transition, perhaps due to the former binding to the HGF receptor to form a unique complex that diversifies the biological functions of HGF.
30572803	5	53	theme	differentiation	943:957	arg1	process					959:965	the differentiation process	939:965	the differentiation process	939:965	A major aspect of hBMSC differentiation is the extracellular matrix (ECM) composition and, in particular, the role of glycosaminoglycans (GAGs) in the differentiation process.
30572803	6	54	theme	conditions/protocols	1033:1052	arg1	influence					998:1006	the influence	994:1006	the influence of four distinct culture conditions/protocols (T1-T4) on GAG composition and hepatic markers	994:1099	In this study, we examine the influence of four distinct culture conditions/protocols (T1-T4) on GAG composition and hepatic markers.
30572803	9	55	theme	early-	1780:1785	arg1	combinations					1802:1813	both early- and late-stage combinations	1775:1813	both early- and late-stage combinations of growth factors	1775:1831	Of the four protocols tested, uniform hepatocyte-like morphological changes, ALB secretion, and glycogen storage were found to be highest with protocol T2, which involves both early- and late-stage combinations of growth factors.
30572803	11	56	theme	hHEP	1996:1999	arg1	profile					1977:1983	The GAG profile	1969:1983	The GAG profile of primary hHEP	1969:1999	The GAG profile of primary hHEP showed an HS-rich ECM, and thus, it may be possible to guide hBMSC differentiation to more mature hepatocytes by controlling the GAG profile expressed by differentiating cells.
30572803	7	57	theme	differentiation	1192:1206	arg1	days					1184:1187	21 days	1181:1187	21 days of differentiation	1181:1206	α-Fetoprotein and hepatocyte nuclear factor-4α were expressed continually over 21 days of differentiation, as indicated by real time quantitative PCR analysis, while albumin (ALB) expression did not begin until day 21.
30572803	9	58	theme	late-stage	1791:1800	arg1	combinations					1802:1813	both early- and late-stage combinations	1775:1813	both early- and late-stage combinations of growth factors	1775:1831	Of the four protocols tested, uniform hepatocyte-like morphological changes, ALB secretion, and glycogen storage were found to be highest with protocol T2, which involves both early- and late-stage combinations of growth factors.
30572803	3	59	theme	embryonic	536:544	arg1	cells					574:578	pluripotent stem cells	557:578	pluripotent stem cells	557:578	Recent advances in stem cell differentiation have opened up the possibility to obtain fully functional hepatocytes from embryonic or induced pluripotent stem cells, or adult stem cells.
30572803	6	60	theme	GAG	1065:1067	arg1	composition					1069:1079	GAG composition	1065:1079	GAG composition	1065:1079	In this study, we examine the influence of four distinct culture conditions/protocols (T1-T4) on GAG composition and hepatic markers.
30572803	5	61	theme	differentiation	816:830	arg1	aspect					800:805	A major aspect	792:805	A major aspect of hBMSC differentiation	792:830	A major aspect of hBMSC differentiation is the extracellular matrix (ECM) composition and, in particular, the role of glycosaminoglycans (GAGs) in the differentiation process.
30572803	5	61	theme	differentiation	816:830	arg1	composition					866:876	the extracellular matrix (ECM) composition	835:876	the extracellular matrix (ECM) composition	835:876	A major aspect of hBMSC differentiation is the extracellular matrix (ECM) composition and, in particular, the role of glycosaminoglycans (GAGs) in the differentiation process.
30572803	9	62	theme	factors	1825:1831	arg1	combinations					1802:1813	both early- and late-stage combinations	1775:1813	both early- and late-stage combinations of growth factors	1775:1831	Of the four protocols tested, uniform hepatocyte-like morphological changes, ALB secretion, and glycogen storage were found to be highest with protocol T2, which involves both early- and late-stage combinations of growth factors.
30572803	4	63	theme	human	630:634	arg1	marrow					641:646	human bone marrow	630:646	human bone marrow mesenchymal stromal cells (hBMSCs)	630:681	With respect to the latter, human bone marrow mesenchymal stromal cells (hBMSCs) can serve as a source of autogenetic and allogenic multipotent stem cells for liver repair and regeneration.
30572803	3	64	theme	stem	569:572	arg1	cells					574:578	pluripotent stem cells	557:578	pluripotent stem cells	557:578	Recent advances in stem cell differentiation have opened up the possibility to obtain fully functional hepatocytes from embryonic or induced pluripotent stem cells, or adult stem cells.
30572803	6	65	theme	hepatic	1085:1091	arg1	markers					1093:1099	hepatic markers	1085:1099	hepatic markers	1085:1099	In this study, we examine the influence of four distinct culture conditions/protocols (T1-T4) on GAG composition and hepatic markers.
30572803	4	66	theme	marrow	641:646	arg1	hBMSCs					675:680	hBMSCs	675:680	hBMSCs	675:680	With respect to the latter, human bone marrow mesenchymal stromal cells (hBMSCs) can serve as a source of autogenetic and allogenic multipotent stem cells for liver repair and regeneration.
30572803	4	66	theme	marrow	641:646	arg1	cells					668:672	human bone marrow mesenchymal stromal cells	630:672	human bone marrow mesenchymal stromal cells (hBMSCs)	630:681	With respect to the latter, human bone marrow mesenchymal stromal cells (hBMSCs) can serve as a source of autogenetic and allogenic multipotent stem cells for liver repair and regeneration.
30572803	4	66	theme	marrow	641:646	arg1	source					698:703	a source	696:703	a source of autogenetic and allogenic multipotent stem cells for liver repair and regeneration	696:789	With respect to the latter, human bone marrow mesenchymal stromal cells (hBMSCs) can serve as a source of autogenetic and allogenic multipotent stem cells for liver repair and regeneration.
30572803	10	67	theme	hBMSC	1863:1867	arg1	ECM					1869:1871	the hBMSC ECM	1859:1871	the hBMSC ECM	1859:1871	The total GAG profile of the hBMSC ECM is rich in heparan sulfate (HS) and hyaluronan, both of which fluctuate during differentiation.
30572803	3	68	theme	induced	549:555	arg1	cells					574:578	pluripotent stem cells	557:578	pluripotent stem cells	557:578	Recent advances in stem cell differentiation have opened up the possibility to obtain fully functional hepatocytes from embryonic or induced pluripotent stem cells, or adult stem cells.
30572803	5	69	theme	major	794:798	arg1	aspect					800:805	A major aspect	792:805	A major aspect of hBMSC differentiation	792:830	A major aspect of hBMSC differentiation is the extracellular matrix (ECM) composition and, in particular, the role of glycosaminoglycans (GAGs) in the differentiation process.
30572803	5	69	theme	major	794:798	arg1	composition					866:876	the extracellular matrix (ECM) composition	835:876	the extracellular matrix (ECM) composition	835:876	A major aspect of hBMSC differentiation is the extracellular matrix (ECM) composition and, in particular, the role of glycosaminoglycans (GAGs) in the differentiation process.
30572803	3	70	theme	adult	584:588	arg1	cells					595:599	adult stem cells	584:599	adult stem cells	584:599	Recent advances in stem cell differentiation have opened up the possibility to obtain fully functional hepatocytes from embryonic or induced pluripotent stem cells, or adult stem cells.
30572803	5	71	theme	extracellular	839:851	arg1	ECM					861:863	ECM	861:863	ECM	861:863	A major aspect of hBMSC differentiation is the extracellular matrix (ECM) composition and, in particular, the role of glycosaminoglycans (GAGs) in the differentiation process.
30572803	5	71	theme	extracellular	839:851	arg1	matrix					853:858	the extracellular matrix	835:858	the extracellular matrix (ECM) composition	835:876	A major aspect of hBMSC differentiation is the extracellular matrix (ECM) composition and, in particular, the role of glycosaminoglycans (GAGs) in the differentiation process.
30572803	9	72	theme	uniform	1634:1640	arg1	changes					1672:1678	uniform hepatocyte-like morphological changes	1634:1678	uniform hepatocyte-like morphological changes	1634:1678	Of the four protocols tested, uniform hepatocyte-like morphological changes, ALB secretion, and glycogen storage were found to be highest with protocol T2, which involves both early- and late-stage combinations of growth factors.
30572803	8	73	theme	activin	1386:1392	arg1	A					1394:1394	activin A	1386:1394	activin A	1386:1394	Hepatocyte growth factor (HGF) appears to be more effective than activin A in promoting hepatic-like cells through the mesenchymal-epithelial transition, perhaps due to the former binding to the HGF receptor to form a unique complex that diversifies the biological functions of HGF.
30572803	1	74	theme	critical	133:140	arg1	need					142:145	a critical need	131:145	a critical need to generate functional hepatocytes to aid in liver repair and regeneration upon availability of a renewable, and potentially personalized, source of human hepatocytes (hHEP)	131:319	There is a critical need to generate functional hepatocytes to aid in liver repair and regeneration upon availability of a renewable, and potentially personalized, source of human hepatocytes (hHEP).
30572803	9	75	theme	morphological	1658:1670	arg1	changes					1672:1678	uniform hepatocyte-like morphological changes	1634:1678	uniform hepatocyte-like morphological changes	1634:1678	Of the four protocols tested, uniform hepatocyte-like morphological changes, ALB secretion, and glycogen storage were found to be highest with protocol T2, which involves both early- and late-stage combinations of growth factors.
30572803	0	76	theme	Mesenchymal	76:86	arg1	Cells					96:100	Adult Human Bone Mesenchymal Stromal Cells	59:100	Adult Human Bone Mesenchymal Stromal Cells	59:100	Remodeling of Glycosaminoglycans During Differentiation of Adult Human Bone Mesenchymal Stromal Cells Toward Hepatocytes.
30572803	5	77	theme	matrix	853:858	arg1	aspect					800:805	A major aspect	792:805	A major aspect of hBMSC differentiation	792:830	A major aspect of hBMSC differentiation is the extracellular matrix (ECM) composition and, in particular, the role of glycosaminoglycans (GAGs) in the differentiation process.
30572803	5	77	theme	matrix	853:858	arg1	composition					866:876	the extracellular matrix (ECM) composition	835:876	the extracellular matrix (ECM) composition	835:876	A major aspect of hBMSC differentiation is the extracellular matrix (ECM) composition and, in particular, the role of glycosaminoglycans (GAGs) in the differentiation process.
30572803	8	78	theme	hepatic-like	1409:1420	arg1	cells					1422:1426	hepatic-like cells	1409:1426	hepatic-like cells	1409:1426	Hepatocyte growth factor (HGF) appears to be more effective than activin A in promoting hepatic-like cells through the mesenchymal-epithelial transition, perhaps due to the former binding to the HGF receptor to form a unique complex that diversifies the biological functions of HGF.
30572803	4	79	theme	stromal	660:666	arg1	hBMSCs					675:680	hBMSCs	675:680	hBMSCs	675:680	With respect to the latter, human bone marrow mesenchymal stromal cells (hBMSCs) can serve as a source of autogenetic and allogenic multipotent stem cells for liver repair and regeneration.
30572803	4	79	theme	stromal	660:666	arg1	cells					668:672	human bone marrow mesenchymal stromal cells	630:672	human bone marrow mesenchymal stromal cells (hBMSCs)	630:681	With respect to the latter, human bone marrow mesenchymal stromal cells (hBMSCs) can serve as a source of autogenetic and allogenic multipotent stem cells for liver repair and regeneration.
30572803	4	79	theme	stromal	660:666	arg1	source					698:703	a source	696:703	a source of autogenetic and allogenic multipotent stem cells for liver repair and regeneration	696:789	With respect to the latter, human bone marrow mesenchymal stromal cells (hBMSCs) can serve as a source of autogenetic and allogenic multipotent stem cells for liver repair and regeneration.
30572803	0	80	theme	Cells	96:100	arg1	Differentiation					40:54	Differentiation	40:54	Differentiation of Adult Human Bone Mesenchymal Stromal Cells Toward Hepatocytes	40:119	Remodeling of Glycosaminoglycans During Differentiation of Adult Human Bone Mesenchymal Stromal Cells Toward Hepatocytes.
30572803	1	81	theme	functional	159:168	arg1	hepatocytes					170:180	functional hepatocytes	159:180	functional hepatocytes	159:180	There is a critical need to generate functional hepatocytes to aid in liver repair and regeneration upon availability of a renewable, and potentially personalized, source of human hepatocytes (hHEP).
30572803	3	82	theme	pluripotent	557:567	arg1	cells					574:578	pluripotent stem cells	557:578	pluripotent stem cells	557:578	Recent advances in stem cell differentiation have opened up the possibility to obtain fully functional hepatocytes from embryonic or induced pluripotent stem cells, or adult stem cells.
30572803	8	83	theme	mesenchymal-epithelial	1440:1461	arg1	transition					1463:1472	the mesenchymal-epithelial transition	1436:1472	the mesenchymal-epithelial transition	1436:1472	Hepatocyte growth factor (HGF) appears to be more effective than activin A in promoting hepatic-like cells through the mesenchymal-epithelial transition, perhaps due to the former binding to the HGF receptor to form a unique complex that diversifies the biological functions of HGF.
30572803	3	84	from	cells	595:599	arg1	hepatocytes					519:529	fully functional hepatocytes	502:529	fully functional hepatocytes from embryonic or induced pluripotent stem cells, or adult stem cells	502:599	Recent advances in stem cell differentiation have opened up the possibility to obtain fully functional hepatocytes from embryonic or induced pluripotent stem cells, or adult stem cells.
30572803	7	85	theme	nuclear	1131:1137	arg1	factor-4α					1139:1147	hepatocyte nuclear factor-4α	1120:1147	hepatocyte nuclear factor-4α	1120:1147	α-Fetoprotein and hepatocyte nuclear factor-4α were expressed continually over 21 days of differentiation, as indicated by real time quantitative PCR analysis, while albumin (ALB) expression did not begin until day 21.
30572803	10	86	from	rich	1876:1879	arg1	HS					1901:1902	HS	1901:1902	HS	1901:1902	The total GAG profile of the hBMSC ECM is rich in heparan sulfate (HS) and hyaluronan, both of which fluctuate during differentiation.
30572803	10	86	from	rich	1876:1879	arg1	hyaluronan					1909:1918	hyaluronan	1909:1918	hyaluronan	1909:1918	The total GAG profile of the hBMSC ECM is rich in heparan sulfate (HS) and hyaluronan, both of which fluctuate during differentiation.
30572803	10	86	from	rich	1876:1879	arg1	sulfate					1892:1898	heparan sulfate	1884:1898	heparan sulfate (HS)	1884:1903	The total GAG profile of the hBMSC ECM is rich in heparan sulfate (HS) and hyaluronan, both of which fluctuate during differentiation.
30572803	4	87	theme	multipotent	734:744	arg1	cells					751:755	autogenetic and allogenic multipotent stem cells	708:755	autogenetic and allogenic multipotent stem cells	708:755	With respect to the latter, human bone marrow mesenchymal stromal cells (hBMSCs) can serve as a source of autogenetic and allogenic multipotent stem cells for liver repair and regeneration.
30572803	3	88	theme	Recent	416:421	arg1	advances					423:430	Recent advances	416:430	Recent advances in stem cell differentiation	416:459	Recent advances in stem cell differentiation have opened up the possibility to obtain fully functional hepatocytes from embryonic or induced pluripotent stem cells, or adult stem cells.
30572803	1	89	theme	source	286:291	arg1	availability					227:238	availability	227:238	availability of a renewable, and potentially personalized, source of human hepatocytes (hHEP)	227:319	There is a critical need to generate functional hepatocytes to aid in liver repair and regeneration upon availability of a renewable, and potentially personalized, source of human hepatocytes (hHEP).
30572803	1	90	theme	human	296:300	arg1	hHEP					315:318	hHEP	315:318	hHEP	315:318	There is a critical need to generate functional hepatocytes to aid in liver repair and regeneration upon availability of a renewable, and potentially personalized, source of human hepatocytes (hHEP).
30572803	1	90	theme	human	296:300	arg1	hepatocytes					302:312	human hepatocytes	296:312	human hepatocytes (hHEP)	296:319	There is a critical need to generate functional hepatocytes to aid in liver repair and regeneration upon availability of a renewable, and potentially personalized, source of human hepatocytes (hHEP).
30572803	7	91	theme	quantitative	1235:1246	arg1	analysis					1252:1259	real time quantitative PCR analysis	1225:1259	real time quantitative PCR analysis	1225:1259	α-Fetoprotein and hepatocyte nuclear factor-4α were expressed continually over 21 days of differentiation, as indicated by real time quantitative PCR analysis, while albumin (ALB) expression did not begin until day 21.
30572803	2	92	theme	human	385:389	arg1	tissue					391:396	human tissue	385:396	human tissue	385:396	Currently, the vast majority of primary hHEP are obtained from human tissue through cadavers.
30572803	0	93	theme	Human	65:69	arg1	Cells					96:100	Adult Human Bone Mesenchymal Stromal Cells	59:100	Adult Human Bone Mesenchymal Stromal Cells	59:100	Remodeling of Glycosaminoglycans During Differentiation of Adult Human Bone Mesenchymal Stromal Cells Toward Hepatocytes.
30572803	10	94	from	hyaluronan	1909:1918	arg1	rich					1876:1879	rich	1876:1879	rich	1876:1879	The total GAG profile of the hBMSC ECM is rich in heparan sulfate (HS) and hyaluronan, both of which fluctuate during differentiation.
30572803	10	94	from	hyaluronan	1909:1918	arg1	profile					1848:1854	The total GAG profile	1834:1854	The total GAG profile of the hBMSC ECM	1834:1871	The total GAG profile of the hBMSC ECM is rich in heparan sulfate (HS) and hyaluronan, both of which fluctuate during differentiation.
30572803	7	95	theme	real	1225:1228	arg1	analysis					1252:1259	real time quantitative PCR analysis	1225:1259	real time quantitative PCR analysis	1225:1259	α-Fetoprotein and hepatocyte nuclear factor-4α were expressed continually over 21 days of differentiation, as indicated by real time quantitative PCR analysis, while albumin (ALB) expression did not begin until day 21.
30572803	8	96	theme	growth	1332:1337	arg1	HGF					1347:1349	HGF	1347:1349	HGF	1347:1349	Hepatocyte growth factor (HGF) appears to be more effective than activin A in promoting hepatic-like cells through the mesenchymal-epithelial transition, perhaps due to the former binding to the HGF receptor to form a unique complex that diversifies the biological functions of HGF.
30572803	8	96	theme	growth	1332:1337	arg1	factor					1339:1344	Hepatocyte growth factor	1321:1344	Hepatocyte growth factor (HGF)	1321:1350	Hepatocyte growth factor (HGF) appears to be more effective than activin A in promoting hepatic-like cells through the mesenchymal-epithelial transition, perhaps due to the former binding to the HGF receptor to form a unique complex that diversifies the biological functions of HGF.
30572803	4	97	theme	cells	751:755	arg1	source					698:703	a source	696:703	a source of autogenetic and allogenic multipotent stem cells for liver repair and regeneration	696:789	With respect to the latter, human bone marrow mesenchymal stromal cells (hBMSCs) can serve as a source of autogenetic and allogenic multipotent stem cells for liver repair and regeneration.
30572803	4	97	theme	cells	751:755	arg1	cells					668:672	human bone marrow mesenchymal stromal cells	630:672	human bone marrow mesenchymal stromal cells (hBMSCs)	630:681	With respect to the latter, human bone marrow mesenchymal stromal cells (hBMSCs) can serve as a source of autogenetic and allogenic multipotent stem cells for liver repair and regeneration.
30572803	4	98	theme	liver	761:765	arg1	repair					767:772	liver repair	761:772	liver repair	761:772	With respect to the latter, human bone marrow mesenchymal stromal cells (hBMSCs) can serve as a source of autogenetic and allogenic multipotent stem cells for liver repair and regeneration.
30572803	3	99	from	advances	423:430	arg1	differentiation					445:459	stem cell differentiation	435:459	stem cell differentiation	435:459	Recent advances in stem cell differentiation have opened up the possibility to obtain fully functional hepatocytes from embryonic or induced pluripotent stem cells, or adult stem cells.
30572803	3	100	theme	functional	508:517	arg1	hepatocytes					519:529	fully functional hepatocytes	502:529	fully functional hepatocytes from embryonic or induced pluripotent stem cells, or adult stem cells	502:599	Recent advances in stem cell differentiation have opened up the possibility to obtain fully functional hepatocytes from embryonic or induced pluripotent stem cells, or adult stem cells.
30572803	8	101	theme	HGF	1599:1601	arg1	functions					1586:1594	the biological functions	1571:1594	the biological functions of HGF	1571:1601	Hepatocyte growth factor (HGF) appears to be more effective than activin A in promoting hepatic-like cells through the mesenchymal-epithelial transition, perhaps due to the former binding to the HGF receptor to form a unique complex that diversifies the biological functions of HGF.
30572803	1	102	theme	renewable	245:253	arg1	source					286:291	a renewable, and potentially personalized, source	243:291	a renewable, and potentially personalized, source of human hepatocytes (hHEP)	243:319	There is a critical need to generate functional hepatocytes to aid in liver repair and regeneration upon availability of a renewable, and potentially personalized, source of human hepatocytes (hHEP).
31569016	4	0	theme	high	879:882	arg1	activities					897:906	high anti-oxidant activities	879:906	high anti-oxidant activities	879:906	In this study, we aimed to develop novel injectable MSC-delivery microgel systems possessing high anti-oxidant activities.
31569016	2	1	theme	major	356:360	arg1	roles					362:366	major roles	356:366	major roles	356:366	In particular, paracrine effects of the transplanted MSCs have been highlighted to play major roles in heart regeneration by secreting multiple growth factors and immune-modulatory cytokines.
31569016	10	2	theme	various	2224:2230	arg1	injuries					2232:2239	various injuries	2224:2239	various injuries	2224:2239	We believe that our novel MSC encapsulation system with GO, alginate, and mild reduction, which exhibits high cell protection capacity (e.g., anti-oxidant activity), will serve as an effective platform for the delivery of stem cells and other therapeutic cell types to treat various injuries and diseases, including MI.
31569016	10	2	theme	various	2224:2230	arg1	MI					2265:2266	MI	2265:2266	MI	2265:2266	We believe that our novel MSC encapsulation system with GO, alginate, and mild reduction, which exhibits high cell protection capacity (e.g., anti-oxidant activity), will serve as an effective platform for the delivery of stem cells and other therapeutic cell types to treat various injuries and diseases, including MI.
31569016	6	3	theme	harsh	1223:1227	arg1	environments					1229:1240	the abovementioned harsh environments	1204:1240	the abovementioned harsh environments	1204:1240	To further enhance the anti-oxidizing activities of the gels, we developed reduced MSC-embedded GO/alginate microgels (i.e., r(GO/alginate)), which have the potential to protect MSCs from the abovementioned harsh environments within MI tissues.
31569016	9	4	theme	infarction	1892:1901	arg1	area					1903:1906	the infarction area	1888:1906	the infarction area	1888:1906	In vivo experiments with acute MI models demonstrated improved therapeutic efficacy of MSC delivery in r(GO/alginate) microgels, exhibiting significant decreases in the infarction area and the improvement of cardiac function.
31569016	8	5	theme	ROS	1637:1639	arg1	scavenging					1641:1650	ROS scavenging	1637:1650	ROS scavenging by the gels and positive paracrine signals from the encapsulated MSCs	1637:1720	Furthermore, cardiomyocytes (CMs), co-cultured with the encapsulated MSCs in transwells with H2O2 treatment, showed higher cell viability and cardiac maturation compared to monolayer cultured CMs, likely due to ROS scavenging by the gels and positive paracrine signals from the encapsulated MSCs.
31569016	4	6	theme	MSC-delivery	838:849	arg1	systems					860:866	novel injectable MSC-delivery microgel systems	821:866	novel injectable MSC-delivery microgel systems possessing high anti-oxidant activities	821:906	In this study, we aimed to develop novel injectable MSC-delivery microgel systems possessing high anti-oxidant activities.
31569016	9	7	from	decreases	1875:1883	arg1	area					1903:1906	the infarction area	1888:1906	the infarction area	1888:1906	In vivo experiments with acute MI models demonstrated improved therapeutic efficacy of MSC delivery in r(GO/alginate) microgels, exhibiting significant decreases in the infarction area and the improvement of cardiac function.
31569016	10	8	theme	anti-oxidant	2091:2102	arg1	activity					2104:2111	anti-oxidant activity	2091:2111	anti-oxidant activity	2091:2111	We believe that our novel MSC encapsulation system with GO, alginate, and mild reduction, which exhibits high cell protection capacity (e.g., anti-oxidant activity), will serve as an effective platform for the delivery of stem cells and other therapeutic cell types to treat various injuries and diseases, including MI.
31569016	3	9	theme	high	721:724	arg1	stress					736:741	high oxidative stress	721:741	high oxidative stress	721:741	Nevertheless, its therapeutic efficacy still remains low, which is strongly associated with low viability and activity of the transplanted stem cells, because the transplanted MSCs are exposed to high shear stress during injection and harsh environments (e.g., high oxidative stress and host immune reactions) post injection.
31569016	0	10	theme	Anti-oxidant	0:11	arg1	activity					13:20	Anti-oxidant activity	0:20	Anti-oxidant activity	0:20	Anti-oxidant activity reinforced reduced graphene oxide/alginate microgels: Mesenchymal stem cell encapsulation and regeneration of infarcted hearts.
31569016	6	11	theme	MI	1249:1250	arg1	tissues					1252:1258	MI tissues	1249:1258	MI tissues	1249:1258	To further enhance the anti-oxidizing activities of the gels, we developed reduced MSC-embedded GO/alginate microgels (i.e., r(GO/alginate)), which have the potential to protect MSCs from the abovementioned harsh environments within MI tissues.
31569016	2	12	theme	MSCs	321:324	arg1	effects					293:299	paracrine effects	283:299	paracrine effects of the transplanted MSCs	283:324	In particular, paracrine effects of the transplanted MSCs have been highlighted to play major roles in heart regeneration by secreting multiple growth factors and immune-modulatory cytokines.
31569016	10	13	dep	exhibits	2045:2052	arg1	activity					2104:2111	anti-oxidant activity	2091:2111	anti-oxidant activity	2091:2111	We believe that our novel MSC encapsulation system with GO, alginate, and mild reduction, which exhibits high cell protection capacity (e.g., anti-oxidant activity), will serve as an effective platform for the delivery of stem cells and other therapeutic cell types to treat various injuries and diseases, including MI.
31569016	7	14	theme	stress	1397:1402	arg1	conditions					1404:1413	oxidative stress conditions	1387:1413	oxidative stress conditions with H2O2	1387:1423	Our in vitro studies demonstrated that the MSCs encapsulated in the r(GO/alginate) microgels showed increased viability under oxidative stress conditions with H2O2.
31569016	9	15	theme	cardiac	1931:1937	arg1	function					1939:1946	cardiac function	1931:1946	cardiac function	1931:1946	In vivo experiments with acute MI models demonstrated improved therapeutic efficacy of MSC delivery in r(GO/alginate) microgels, exhibiting significant decreases in the infarction area and the improvement of cardiac function.
31569016	3	16	theme	host	747:750	arg1	reactions					759:767	host immune reactions	747:767	host immune reactions	747:767	Nevertheless, its therapeutic efficacy still remains low, which is strongly associated with low viability and activity of the transplanted stem cells, because the transplanted MSCs are exposed to high shear stress during injection and harsh environments (e.g., high oxidative stress and host immune reactions) post injection.
31569016	0	17	theme	hearts	142:147	arg1	cell					93:96	Mesenchymal stem cell encapsulation and regeneration	76:127	cell	93:96	Anti-oxidant activity reinforced reduced graphene oxide/alginate microgels: Mesenchymal stem cell encapsulation and regeneration of infarcted hearts.
31569016	0	17	theme	hearts	142:147	arg1	regeneration					116:127	regeneration	116:127	regeneration	116:127	Anti-oxidant activity reinforced reduced graphene oxide/alginate microgels: Mesenchymal stem cell encapsulation and regeneration of infarcted hearts.
31569016	0	17	theme	hearts	142:147	arg1	microgels					65:73	reduced graphene oxide/alginate microgels	33:73	reduced graphene oxide/alginate microgels: Mesenchymal stem cell encapsulation and regeneration of infarcted hearts	33:147	Anti-oxidant activity reinforced reduced graphene oxide/alginate microgels: Mesenchymal stem cell encapsulation and regeneration of infarcted hearts.
31569016	1	18	theme	heart	221:225	arg1	tissues					227:233	heart tissues	221:233	heart tissues post myocardial infarction (MI)	221:265	Mesenchymal stem cell (MSC) transplantation is promising for repairing heart tissues post myocardial infarction (MI).
31569016	7	19	theme	increased	1361:1369	arg1	viability					1371:1379	increased viability	1361:1379	increased viability	1361:1379	Our in vitro studies demonstrated that the MSCs encapsulated in the r(GO/alginate) microgels showed increased viability under oxidative stress conditions with H2O2.
31569016	10	20	theme	effective	2132:2140	arg1	platform					2142:2149	an effective platform	2129:2149	an effective platform for the delivery of stem cells and other therapeutic cell types	2129:2213	We believe that our novel MSC encapsulation system with GO, alginate, and mild reduction, which exhibits high cell protection capacity (e.g., anti-oxidant activity), will serve as an effective platform for the delivery of stem cells and other therapeutic cell types to treat various injuries and diseases, including MI.
31569016	10	20	theme	effective	2132:2140	arg1	system					1993:1998	our novel MSC encapsulation system	1965:1998	our novel MSC encapsulation system with GO, alginate, and mild reduction, which exhibits high cell protection capacity (e.g., anti-oxidant activity),	1965:2113	We believe that our novel MSC encapsulation system with GO, alginate, and mild reduction, which exhibits high cell protection capacity (e.g., anti-oxidant activity), will serve as an effective platform for the delivery of stem cells and other therapeutic cell types to treat various injuries and diseases, including MI.
31569016	10	21	theme	MSC	1975:1977	arg1	platform					2142:2149	an effective platform	2129:2149	an effective platform for the delivery of stem cells and other therapeutic cell types	2129:2213	We believe that our novel MSC encapsulation system with GO, alginate, and mild reduction, which exhibits high cell protection capacity (e.g., anti-oxidant activity), will serve as an effective platform for the delivery of stem cells and other therapeutic cell types to treat various injuries and diseases, including MI.
31569016	10	21	theme	MSC	1975:1977	arg1	system					1993:1998	our novel MSC encapsulation system	1965:1998	our novel MSC encapsulation system with GO, alginate, and mild reduction, which exhibits high cell protection capacity (e.g., anti-oxidant activity),	1965:2113	We believe that our novel MSC encapsulation system with GO, alginate, and mild reduction, which exhibits high cell protection capacity (e.g., anti-oxidant activity), will serve as an effective platform for the delivery of stem cells and other therapeutic cell types to treat various injuries and diseases, including MI.
31569016	5	22	theme	graphene	947:954	arg1	oxide					956:960	graphene oxide	947:960	graphene oxide (GO)/alginate composite microgels	947:994	Specifically, we encapsulated MSCs in graphene oxide (GO)/alginate composite microgels by electrospraying.
31569016	6	23	theme	anti-oxidizing	1039:1052	arg1	activities					1054:1063	the anti-oxidizing activities	1035:1063	the anti-oxidizing activities of the gels	1035:1075	To further enhance the anti-oxidizing activities of the gels, we developed reduced MSC-embedded GO/alginate microgels (i.e., r(GO/alginate)), which have the potential to protect MSCs from the abovementioned harsh environments within MI tissues.
31569016	10	24	theme	cell	2059:2062	arg1	capacity					2075:2082	high cell protection capacity	2054:2082	high cell protection capacity	2054:2082	We believe that our novel MSC encapsulation system with GO, alginate, and mild reduction, which exhibits high cell protection capacity (e.g., anti-oxidant activity), will serve as an effective platform for the delivery of stem cells and other therapeutic cell types to treat various injuries and diseases, including MI.
31569016	10	25	with	system	1993:1998	arg1	alginate					2009:2016	alginate	2009:2016	alginate	2009:2016	We believe that our novel MSC encapsulation system with GO, alginate, and mild reduction, which exhibits high cell protection capacity (e.g., anti-oxidant activity), will serve as an effective platform for the delivery of stem cells and other therapeutic cell types to treat various injuries and diseases, including MI.
31569016	10	25	with	system	1993:1998	arg1	GO					2005:2006	GO	2005:2006	GO	2005:2006	We believe that our novel MSC encapsulation system with GO, alginate, and mild reduction, which exhibits high cell protection capacity (e.g., anti-oxidant activity), will serve as an effective platform for the delivery of stem cells and other therapeutic cell types to treat various injuries and diseases, including MI.
31569016	10	25	with	system	1993:1998	arg1	reduction					2028:2036	mild reduction	2023:2036	mild reduction	2023:2036	We believe that our novel MSC encapsulation system with GO, alginate, and mild reduction, which exhibits high cell protection capacity (e.g., anti-oxidant activity), will serve as an effective platform for the delivery of stem cells and other therapeutic cell types to treat various injuries and diseases, including MI.
31569016	0	26	theme	Mesenchymal	76:86	arg1	cell					93:96	Mesenchymal stem cell encapsulation and regeneration	76:127	cell	93:96	Anti-oxidant activity reinforced reduced graphene oxide/alginate microgels: Mesenchymal stem cell encapsulation and regeneration of infarcted hearts.
31569016	0	26	theme	Mesenchymal	76:86	arg1	microgels					65:73	reduced graphene oxide/alginate microgels	33:73	reduced graphene oxide/alginate microgels: Mesenchymal stem cell encapsulation and regeneration of infarcted hearts	33:147	Anti-oxidant activity reinforced reduced graphene oxide/alginate microgels: Mesenchymal stem cell encapsulation and regeneration of infarcted hearts.
31569016	9	27	theme	MI	1754:1755	arg1	models					1757:1762	acute MI models	1748:1762	acute MI models	1748:1762	In vivo experiments with acute MI models demonstrated improved therapeutic efficacy of MSC delivery in r(GO/alginate) microgels, exhibiting significant decreases in the infarction area and the improvement of cardiac function.
31569016	5	28	dep	oxide	956:960	arg1	GO					963:964	GO	963:964	GO	963:964	Specifically, we encapsulated MSCs in graphene oxide (GO)/alginate composite microgels by electrospraying.
31569016	4	29	contain	possessing	868:877	arg2	activities					897:906	high anti-oxidant activities	879:906	high anti-oxidant activities	879:906	In this study, we aimed to develop novel injectable MSC-delivery microgel systems possessing high anti-oxidant activities.
31569016	4	29	contain	possessing	868:877	arg1	systems					860:866	novel injectable MSC-delivery microgel systems	821:866	novel injectable MSC-delivery microgel systems possessing high anti-oxidant activities	821:906	In this study, we aimed to develop novel injectable MSC-delivery microgel systems possessing high anti-oxidant activities.
31569016	9	30	from	improvement	1916:1926	arg1	area					1903:1906	the infarction area	1888:1906	the infarction area	1888:1906	In vivo experiments with acute MI models demonstrated improved therapeutic efficacy of MSC delivery in r(GO/alginate) microgels, exhibiting significant decreases in the infarction area and the improvement of cardiac function.
31569016	5	31	theme	composite	976:984	arg1	microgels					986:994	graphene oxide (GO)/alginate composite microgels	947:994	graphene oxide (GO)/alginate composite microgels	947:994	Specifically, we encapsulated MSCs in graphene oxide (GO)/alginate composite microgels by electrospraying.
31569016	1	32	theme	stem	162:165	arg1	transplantation					178:192	Mesenchymal stem cell (MSC) transplantation	150:192	Mesenchymal stem cell (MSC) transplantation	150:192	Mesenchymal stem cell (MSC) transplantation is promising for repairing heart tissues post myocardial infarction (MI).
31569016	9	33	theme	therapeutic	1786:1796	arg1	efficacy					1798:1805	improved therapeutic efficacy	1777:1805	improved therapeutic efficacy of MSC delivery in r(GO/alginate) microgels	1777:1849	In vivo experiments with acute MI models demonstrated improved therapeutic efficacy of MSC delivery in r(GO/alginate) microgels, exhibiting significant decreases in the infarction area and the improvement of cardiac function.
31569016	8	34	theme	H2O2	1519:1522	arg1	treatment					1524:1532	H2O2 treatment	1519:1532	H2O2 treatment	1519:1532	Furthermore, cardiomyocytes (CMs), co-cultured with the encapsulated MSCs in transwells with H2O2 treatment, showed higher cell viability and cardiac maturation compared to monolayer cultured CMs, likely due to ROS scavenging by the gels and positive paracrine signals from the encapsulated MSCs.
31569016	10	35	theme	stem	2171:2174	arg1	cells					2176:2180	stem cells	2171:2180	stem cells	2171:2180	We believe that our novel MSC encapsulation system with GO, alginate, and mild reduction, which exhibits high cell protection capacity (e.g., anti-oxidant activity), will serve as an effective platform for the delivery of stem cells and other therapeutic cell types to treat various injuries and diseases, including MI.
31569016	2	36	theme	multiple	403:410	arg1	factors					419:425	multiple growth factors	403:425	multiple growth factors	403:425	In particular, paracrine effects of the transplanted MSCs have been highlighted to play major roles in heart regeneration by secreting multiple growth factors and immune-modulatory cytokines.
31569016	9	37	dep	In	1723:1724	arg1	vivo					1726:1729	vivo	1726:1729	vivo	1726:1729	In vivo experiments with acute MI models demonstrated improved therapeutic efficacy of MSC delivery in r(GO/alginate) microgels, exhibiting significant decreases in the infarction area and the improvement of cardiac function.
31569016	6	38	theme	reduced	1091:1097	arg1	microgels					1124:1132	reduced MSC-embedded GO/alginate microgels	1091:1132	reduced MSC-embedded GO/alginate microgels (i.e., r(GO/alginate))	1091:1155	To further enhance the anti-oxidizing activities of the gels, we developed reduced MSC-embedded GO/alginate microgels (i.e., r(GO/alginate)), which have the potential to protect MSCs from the abovementioned harsh environments within MI tissues.
31569016	10	39	theme	types	2209:2213	arg1	delivery					2159:2166	the delivery	2155:2166	the delivery of stem cells and other therapeutic cell types	2155:2213	We believe that our novel MSC encapsulation system with GO, alginate, and mild reduction, which exhibits high cell protection capacity (e.g., anti-oxidant activity), will serve as an effective platform for the delivery of stem cells and other therapeutic cell types to treat various injuries and diseases, including MI.
31569016	7	40	with	conditions	1404:1413	arg1	H2O2					1420:1423	H2O2	1420:1423	H2O2	1420:1423	Our in vitro studies demonstrated that the MSCs encapsulated in the r(GO/alginate) microgels showed increased viability under oxidative stress conditions with H2O2.
31569016	0	41	dep	cell	93:96	arg1	encapsulation					98:110	encapsulation	98:110	encapsulation	98:110	Anti-oxidant activity reinforced reduced graphene oxide/alginate microgels: Mesenchymal stem cell encapsulation and regeneration of infarcted hearts.
31569016	9	42	theme	delivery	1814:1821	arg1	efficacy					1798:1805	improved therapeutic efficacy	1777:1805	improved therapeutic efficacy of MSC delivery in r(GO/alginate) microgels	1777:1849	In vivo experiments with acute MI models demonstrated improved therapeutic efficacy of MSC delivery in r(GO/alginate) microgels, exhibiting significant decreases in the infarction area and the improvement of cardiac function.
31569016	8	43	theme	higher	1542:1547	arg1	viability					1554:1562	higher cell viability	1542:1562	higher cell viability	1542:1562	Furthermore, cardiomyocytes (CMs), co-cultured with the encapsulated MSCs in transwells with H2O2 treatment, showed higher cell viability and cardiac maturation compared to monolayer cultured CMs, likely due to ROS scavenging by the gels and positive paracrine signals from the encapsulated MSCs.
31569016	10	44	theme	therapeutic	2192:2202	arg1	types					2209:2213	other therapeutic cell types	2186:2213	other therapeutic cell types	2186:2213	We believe that our novel MSC encapsulation system with GO, alginate, and mild reduction, which exhibits high cell protection capacity (e.g., anti-oxidant activity), will serve as an effective platform for the delivery of stem cells and other therapeutic cell types to treat various injuries and diseases, including MI.
31569016	2	45	theme	immune-modulatory	431:447	arg1	cytokines					449:457	immune-modulatory cytokines	431:457	immune-modulatory cytokines	431:457	In particular, paracrine effects of the transplanted MSCs have been highlighted to play major roles in heart regeneration by secreting multiple growth factors and immune-modulatory cytokines.
31569016	8	46	from	MSCs	1495:1498	arg1	transwells					1503:1512	transwells	1503:1512	transwells with H2O2 treatment	1503:1532	Furthermore, cardiomyocytes (CMs), co-cultured with the encapsulated MSCs in transwells with H2O2 treatment, showed higher cell viability and cardiac maturation compared to monolayer cultured CMs, likely due to ROS scavenging by the gels and positive paracrine signals from the encapsulated MSCs.
31569016	3	47	theme	low	552:554	arg1	viability					556:564	low viability	552:564	low viability	552:564	Nevertheless, its therapeutic efficacy still remains low, which is strongly associated with low viability and activity of the transplanted stem cells, because the transplanted MSCs are exposed to high shear stress during injection and harsh environments (e.g., high oxidative stress and host immune reactions) post injection.
31569016	6	48	theme	gels	1072:1075	arg1	activities					1054:1063	the anti-oxidizing activities	1035:1063	the anti-oxidizing activities of the gels	1035:1075	To further enhance the anti-oxidizing activities of the gels, we developed reduced MSC-embedded GO/alginate microgels (i.e., r(GO/alginate)), which have the potential to protect MSCs from the abovementioned harsh environments within MI tissues.
31569016	9	49	theme	r	1826:1826	arg1	microgels					1841:1849	r(GO/alginate) microgels	1826:1849	r(GO/alginate) microgels	1826:1849	In vivo experiments with acute MI models demonstrated improved therapeutic efficacy of MSC delivery in r(GO/alginate) microgels, exhibiting significant decreases in the infarction area and the improvement of cardiac function.
31569016	8	50	from	MSCs	1717:1720	arg1	gels					1659:1662	gels	1659:1662	gels	1659:1662	Furthermore, cardiomyocytes (CMs), co-cultured with the encapsulated MSCs in transwells with H2O2 treatment, showed higher cell viability and cardiac maturation compared to monolayer cultured CMs, likely due to ROS scavenging by the gels and positive paracrine signals from the encapsulated MSCs.
31569016	8	50	from	MSCs	1717:1720	arg1	signals					1687:1693	positive paracrine signals	1668:1693	positive paracrine signals	1668:1693	Furthermore, cardiomyocytes (CMs), co-cultured with the encapsulated MSCs in transwells with H2O2 treatment, showed higher cell viability and cardiac maturation compared to monolayer cultured CMs, likely due to ROS scavenging by the gels and positive paracrine signals from the encapsulated MSCs.
31569016	0	51	theme	graphene	41:48	arg1	cell					93:96	Mesenchymal stem cell encapsulation and regeneration	76:127	cell	93:96	Anti-oxidant activity reinforced reduced graphene oxide/alginate microgels: Mesenchymal stem cell encapsulation and regeneration of infarcted hearts.
31569016	0	51	theme	graphene	41:48	arg1	regeneration					116:127	regeneration	116:127	regeneration	116:127	Anti-oxidant activity reinforced reduced graphene oxide/alginate microgels: Mesenchymal stem cell encapsulation and regeneration of infarcted hearts.
31569016	0	51	theme	graphene	41:48	arg1	microgels					65:73	reduced graphene oxide/alginate microgels	33:73	reduced graphene oxide/alginate microgels: Mesenchymal stem cell encapsulation and regeneration of infarcted hearts	33:147	Anti-oxidant activity reinforced reduced graphene oxide/alginate microgels: Mesenchymal stem cell encapsulation and regeneration of infarcted hearts.
31569016	0	52	dep	microgels	65:73	arg1	cell					93:96	Mesenchymal stem cell encapsulation and regeneration	76:127	cell	93:96	Anti-oxidant activity reinforced reduced graphene oxide/alginate microgels: Mesenchymal stem cell encapsulation and regeneration of infarcted hearts.
31569016	0	52	dep	microgels	65:73	arg1	regeneration					116:127	regeneration	116:127	regeneration	116:127	Anti-oxidant activity reinforced reduced graphene oxide/alginate microgels: Mesenchymal stem cell encapsulation and regeneration of infarcted hearts.
31569016	0	52	dep	microgels	65:73	arg1	microgels					65:73	reduced graphene oxide/alginate microgels	33:73	reduced graphene oxide/alginate microgels: Mesenchymal stem cell encapsulation and regeneration of infarcted hearts	33:147	Anti-oxidant activity reinforced reduced graphene oxide/alginate microgels: Mesenchymal stem cell encapsulation and regeneration of infarcted hearts.
31569016	8	53	theme	cardiac	1568:1574	arg1	maturation					1576:1585	cardiac maturation	1568:1585	cardiac maturation	1568:1585	Furthermore, cardiomyocytes (CMs), co-cultured with the encapsulated MSCs in transwells with H2O2 treatment, showed higher cell viability and cardiac maturation compared to monolayer cultured CMs, likely due to ROS scavenging by the gels and positive paracrine signals from the encapsulated MSCs.
31569016	3	54	theme	high	656:659	arg1	stress					667:672	high shear stress	656:672	high shear stress	656:672	Nevertheless, its therapeutic efficacy still remains low, which is strongly associated with low viability and activity of the transplanted stem cells, because the transplanted MSCs are exposed to high shear stress during injection and harsh environments (e.g., high oxidative stress and host immune reactions) post injection.
31569016	4	55	theme	novel	821:825	arg1	systems					860:866	novel injectable MSC-delivery microgel systems	821:866	novel injectable MSC-delivery microgel systems possessing high anti-oxidant activities	821:906	In this study, we aimed to develop novel injectable MSC-delivery microgel systems possessing high anti-oxidant activities.
31569016	3	56	dep	stress	736:741	arg1	e.g.					715:718	e.g.	715:718	e.g.	715:718	Nevertheless, its therapeutic efficacy still remains low, which is strongly associated with low viability and activity of the transplanted stem cells, because the transplanted MSCs are exposed to high shear stress during injection and harsh environments (e.g., high oxidative stress and host immune reactions) post injection.
31569016	8	57	theme	cultured	1609:1616	arg1	CMs					1618:1620	monolayer cultured CMs	1599:1620	monolayer cultured CMs	1599:1620	Furthermore, cardiomyocytes (CMs), co-cultured with the encapsulated MSCs in transwells with H2O2 treatment, showed higher cell viability and cardiac maturation compared to monolayer cultured CMs, likely due to ROS scavenging by the gels and positive paracrine signals from the encapsulated MSCs.
31569016	10	58	theme	high	2054:2057	arg1	capacity					2075:2082	high cell protection capacity	2054:2082	high cell protection capacity	2054:2082	We believe that our novel MSC encapsulation system with GO, alginate, and mild reduction, which exhibits high cell protection capacity (e.g., anti-oxidant activity), will serve as an effective platform for the delivery of stem cells and other therapeutic cell types to treat various injuries and diseases, including MI.
31569016	3	59	theme	harsh	695:699	arg1	environments					701:712	harsh environments	695:712	harsh environments (e.g., high oxidative stress and host immune reactions)	695:768	Nevertheless, its therapeutic efficacy still remains low, which is strongly associated with low viability and activity of the transplanted stem cells, because the transplanted MSCs are exposed to high shear stress during injection and harsh environments (e.g., high oxidative stress and host immune reactions) post injection.
31569016	6	60	dep	r	1141:1141	arg1	i.e.					1135:1138	i.e.	1135:1138	i.e.	1135:1138	To further enhance the anti-oxidizing activities of the gels, we developed reduced MSC-embedded GO/alginate microgels (i.e., r(GO/alginate)), which have the potential to protect MSCs from the abovementioned harsh environments within MI tissues.
31569016	1	61	theme	myocardial	240:249	arg1	MI					263:264	MI	263:264	MI	263:264	Mesenchymal stem cell (MSC) transplantation is promising for repairing heart tissues post myocardial infarction (MI).
31569016	1	61	theme	myocardial	240:249	arg1	infarction					251:260	myocardial infarction	240:260	myocardial infarction (MI)	240:265	Mesenchymal stem cell (MSC) transplantation is promising for repairing heart tissues post myocardial infarction (MI).
31569016	8	62	theme	monolayer	1599:1607	arg1	CMs					1618:1620	monolayer cultured CMs	1599:1620	monolayer cultured CMs	1599:1620	Furthermore, cardiomyocytes (CMs), co-cultured with the encapsulated MSCs in transwells with H2O2 treatment, showed higher cell viability and cardiac maturation compared to monolayer cultured CMs, likely due to ROS scavenging by the gels and positive paracrine signals from the encapsulated MSCs.
31569016	3	63	theme	therapeutic	478:488	arg1	efficacy					490:497	its therapeutic efficacy	474:497	its therapeutic efficacy	474:497	Nevertheless, its therapeutic efficacy still remains low, which is strongly associated with low viability and activity of the transplanted stem cells, because the transplanted MSCs are exposed to high shear stress during injection and harsh environments (e.g., high oxidative stress and host immune reactions) post injection.
31569016	2	64	theme	heart	371:375	arg1	regeneration					377:388	heart regeneration	371:388	heart regeneration	371:388	In particular, paracrine effects of the transplanted MSCs have been highlighted to play major roles in heart regeneration by secreting multiple growth factors and immune-modulatory cytokines.
31569016	10	65	dep	activity	2104:2111	arg1	e.g.					2085:2088	e.g.	2085:2088	e.g.	2085:2088	We believe that our novel MSC encapsulation system with GO, alginate, and mild reduction, which exhibits high cell protection capacity (e.g., anti-oxidant activity), will serve as an effective platform for the delivery of stem cells and other therapeutic cell types to treat various injuries and diseases, including MI.
31569016	6	66	theme	GO/alginate	1112:1122	arg1	microgels					1124:1132	reduced MSC-embedded GO/alginate microgels	1091:1132	reduced MSC-embedded GO/alginate microgels (i.e., r(GO/alginate))	1091:1155	To further enhance the anti-oxidizing activities of the gels, we developed reduced MSC-embedded GO/alginate microgels (i.e., r(GO/alginate)), which have the potential to protect MSCs from the abovementioned harsh environments within MI tissues.
31569016	10	67	theme	protection	2064:2073	arg1	capacity					2075:2082	high cell protection capacity	2054:2082	high cell protection capacity	2054:2082	We believe that our novel MSC encapsulation system with GO, alginate, and mild reduction, which exhibits high cell protection capacity (e.g., anti-oxidant activity), will serve as an effective platform for the delivery of stem cells and other therapeutic cell types to treat various injuries and diseases, including MI.
31569016	8	68	theme	paracrine	1677:1685	arg1	signals					1687:1693	positive paracrine signals	1668:1693	positive paracrine signals	1668:1693	Furthermore, cardiomyocytes (CMs), co-cultured with the encapsulated MSCs in transwells with H2O2 treatment, showed higher cell viability and cardiac maturation compared to monolayer cultured CMs, likely due to ROS scavenging by the gels and positive paracrine signals from the encapsulated MSCs.
31569016	4	69	theme	anti-oxidant	884:895	arg1	activities					897:906	high anti-oxidant activities	879:906	high anti-oxidant activities	879:906	In this study, we aimed to develop novel injectable MSC-delivery microgel systems possessing high anti-oxidant activities.
31569016	2	70	theme	paracrine	283:291	arg1	effects					293:299	paracrine effects	283:299	paracrine effects of the transplanted MSCs	283:324	In particular, paracrine effects of the transplanted MSCs have been highlighted to play major roles in heart regeneration by secreting multiple growth factors and immune-modulatory cytokines.
31569016	4	71	theme	injectable	827:836	arg1	systems					860:866	novel injectable MSC-delivery microgel systems	821:866	novel injectable MSC-delivery microgel systems possessing high anti-oxidant activities	821:906	In this study, we aimed to develop novel injectable MSC-delivery microgel systems possessing high anti-oxidant activities.
31569016	3	72	theme	oxidative	726:734	arg1	stress					736:741	high oxidative stress	721:741	high oxidative stress	721:741	Nevertheless, its therapeutic efficacy still remains low, which is strongly associated with low viability and activity of the transplanted stem cells, because the transplanted MSCs are exposed to high shear stress during injection and harsh environments (e.g., high oxidative stress and host immune reactions) post injection.
31569016	3	73	theme	transplanted	586:597	arg1	cells					604:608	the transplanted stem cells	582:608	the transplanted stem cells	582:608	Nevertheless, its therapeutic efficacy still remains low, which is strongly associated with low viability and activity of the transplanted stem cells, because the transplanted MSCs are exposed to high shear stress during injection and harsh environments (e.g., high oxidative stress and host immune reactions) post injection.
31569016	8	74	theme	encapsulated	1704:1715	arg1	MSCs					1717:1720	the encapsulated MSCs	1700:1720	the encapsulated MSCs	1700:1720	Furthermore, cardiomyocytes (CMs), co-cultured with the encapsulated MSCs in transwells with H2O2 treatment, showed higher cell viability and cardiac maturation compared to monolayer cultured CMs, likely due to ROS scavenging by the gels and positive paracrine signals from the encapsulated MSCs.
31569016	4	75	theme	microgel	851:858	arg1	systems					860:866	novel injectable MSC-delivery microgel systems	821:866	novel injectable MSC-delivery microgel systems possessing high anti-oxidant activities	821:906	In this study, we aimed to develop novel injectable MSC-delivery microgel systems possessing high anti-oxidant activities.
31569016	2	76	theme	transplanted	308:319	arg1	MSCs					321:324	the transplanted MSCs	304:324	the transplanted MSCs	304:324	In particular, paracrine effects of the transplanted MSCs have been highlighted to play major roles in heart regeneration by secreting multiple growth factors and immune-modulatory cytokines.
31569016	3	77	theme	stem	599:602	arg1	cells					604:608	the transplanted stem cells	582:608	the transplanted stem cells	582:608	Nevertheless, its therapeutic efficacy still remains low, which is strongly associated with low viability and activity of the transplanted stem cells, because the transplanted MSCs are exposed to high shear stress during injection and harsh environments (e.g., high oxidative stress and host immune reactions) post injection.
31569016	9	78	theme	function	1939:1946	arg1	improvement					1916:1926	the improvement	1912:1926	the improvement of cardiac function	1912:1946	In vivo experiments with acute MI models demonstrated improved therapeutic efficacy of MSC delivery in r(GO/alginate) microgels, exhibiting significant decreases in the infarction area and the improvement of cardiac function.
31569016	9	78	theme	function	1939:1946	arg1	decreases					1875:1883	significant decreases	1863:1883	significant decreases in the infarction area	1863:1906	In vivo experiments with acute MI models demonstrated improved therapeutic efficacy of MSC delivery in r(GO/alginate) microgels, exhibiting significant decreases in the infarction area and the improvement of cardiac function.
31569016	3	79	theme	immune	752:757	arg1	reactions					759:767	host immune reactions	747:767	host immune reactions	747:767	Nevertheless, its therapeutic efficacy still remains low, which is strongly associated with low viability and activity of the transplanted stem cells, because the transplanted MSCs are exposed to high shear stress during injection and harsh environments (e.g., high oxidative stress and host immune reactions) post injection.
31569016	7	80	theme	oxidative	1387:1395	arg1	conditions					1404:1413	oxidative stress conditions	1387:1413	oxidative stress conditions with H2O2	1387:1423	Our in vitro studies demonstrated that the MSCs encapsulated in the r(GO/alginate) microgels showed increased viability under oxidative stress conditions with H2O2.
31569016	10	81	theme	encapsulation	1979:1991	arg1	platform					2142:2149	an effective platform	2129:2149	an effective platform for the delivery of stem cells and other therapeutic cell types	2129:2213	We believe that our novel MSC encapsulation system with GO, alginate, and mild reduction, which exhibits high cell protection capacity (e.g., anti-oxidant activity), will serve as an effective platform for the delivery of stem cells and other therapeutic cell types to treat various injuries and diseases, including MI.
31569016	10	81	theme	encapsulation	1979:1991	arg1	system					1993:1998	our novel MSC encapsulation system	1965:1998	our novel MSC encapsulation system with GO, alginate, and mild reduction, which exhibits high cell protection capacity (e.g., anti-oxidant activity),	1965:2113	We believe that our novel MSC encapsulation system with GO, alginate, and mild reduction, which exhibits high cell protection capacity (e.g., anti-oxidant activity), will serve as an effective platform for the delivery of stem cells and other therapeutic cell types to treat various injuries and diseases, including MI.
31569016	3	82	theme	cells	604:608	arg1	activity					570:577	activity	570:577	activity	570:577	Nevertheless, its therapeutic efficacy still remains low, which is strongly associated with low viability and activity of the transplanted stem cells, because the transplanted MSCs are exposed to high shear stress during injection and harsh environments (e.g., high oxidative stress and host immune reactions) post injection.
31569016	3	82	theme	cells	604:608	arg1	viability					556:564	low viability	552:564	low viability	552:564	Nevertheless, its therapeutic efficacy still remains low, which is strongly associated with low viability and activity of the transplanted stem cells, because the transplanted MSCs are exposed to high shear stress during injection and harsh environments (e.g., high oxidative stress and host immune reactions) post injection.
31569016	9	83	theme	In	1723:1724	arg1	experiments					1731:1741	In vivo experiments	1723:1741	In vivo experiments with acute MI models	1723:1762	In vivo experiments with acute MI models demonstrated improved therapeutic efficacy of MSC delivery in r(GO/alginate) microgels, exhibiting significant decreases in the infarction area and the improvement of cardiac function.
31569016	6	84	theme	abovementioned	1208:1221	arg1	environments					1229:1240	the abovementioned harsh environments	1204:1240	the abovementioned harsh environments	1204:1240	To further enhance the anti-oxidizing activities of the gels, we developed reduced MSC-embedded GO/alginate microgels (i.e., r(GO/alginate)), which have the potential to protect MSCs from the abovementioned harsh environments within MI tissues.
31569016	10	85	theme	novel	1969:1973	arg1	platform					2142:2149	an effective platform	2129:2149	an effective platform for the delivery of stem cells and other therapeutic cell types	2129:2213	We believe that our novel MSC encapsulation system with GO, alginate, and mild reduction, which exhibits high cell protection capacity (e.g., anti-oxidant activity), will serve as an effective platform for the delivery of stem cells and other therapeutic cell types to treat various injuries and diseases, including MI.
31569016	10	85	theme	novel	1969:1973	arg1	system					1993:1998	our novel MSC encapsulation system	1965:1998	our novel MSC encapsulation system with GO, alginate, and mild reduction, which exhibits high cell protection capacity (e.g., anti-oxidant activity),	1965:2113	We believe that our novel MSC encapsulation system with GO, alginate, and mild reduction, which exhibits high cell protection capacity (e.g., anti-oxidant activity), will serve as an effective platform for the delivery of stem cells and other therapeutic cell types to treat various injuries and diseases, including MI.
31569016	1	86	theme	Mesenchymal	150:160	arg1	transplantation					178:192	Mesenchymal stem cell (MSC) transplantation	150:192	Mesenchymal stem cell (MSC) transplantation	150:192	Mesenchymal stem cell (MSC) transplantation is promising for repairing heart tissues post myocardial infarction (MI).
31569016	8	87	theme	encapsulated	1482:1493	arg1	MSCs					1495:1498	the encapsulated MSCs	1478:1498	the encapsulated MSCs in transwells with H2O2 treatment	1478:1532	Furthermore, cardiomyocytes (CMs), co-cultured with the encapsulated MSCs in transwells with H2O2 treatment, showed higher cell viability and cardiac maturation compared to monolayer cultured CMs, likely due to ROS scavenging by the gels and positive paracrine signals from the encapsulated MSCs.
31569016	7	88	dep	in	1265:1266	arg1	vitro					1268:1272	vitro	1268:1272	vitro	1268:1272	Our in vitro studies demonstrated that the MSCs encapsulated in the r(GO/alginate) microgels showed increased viability under oxidative stress conditions with H2O2.
31569016	8	89	dep	gels	1659:1662	arg1	the					1655:1657	the	1655:1657	the	1655:1657	Furthermore, cardiomyocytes (CMs), co-cultured with the encapsulated MSCs in transwells with H2O2 treatment, showed higher cell viability and cardiac maturation compared to monolayer cultured CMs, likely due to ROS scavenging by the gels and positive paracrine signals from the encapsulated MSCs.
31569016	0	90	theme	stem	88:91	arg1	cell					93:96	Mesenchymal stem cell encapsulation and regeneration	76:127	cell	93:96	Anti-oxidant activity reinforced reduced graphene oxide/alginate microgels: Mesenchymal stem cell encapsulation and regeneration of infarcted hearts.
31569016	0	90	theme	stem	88:91	arg1	microgels					65:73	reduced graphene oxide/alginate microgels	33:73	reduced graphene oxide/alginate microgels: Mesenchymal stem cell encapsulation and regeneration of infarcted hearts	33:147	Anti-oxidant activity reinforced reduced graphene oxide/alginate microgels: Mesenchymal stem cell encapsulation and regeneration of infarcted hearts.
31569016	3	91	theme	transplanted	623:634	arg1	MSCs					636:639	the transplanted MSCs	619:639	the transplanted MSCs	619:639	Nevertheless, its therapeutic efficacy still remains low, which is strongly associated with low viability and activity of the transplanted stem cells, because the transplanted MSCs are exposed to high shear stress during injection and harsh environments (e.g., high oxidative stress and host immune reactions) post injection.
31569016	1	92	theme	cell	167:170	arg1	transplantation					178:192	Mesenchymal stem cell (MSC) transplantation	150:192	Mesenchymal stem cell (MSC) transplantation	150:192	Mesenchymal stem cell (MSC) transplantation is promising for repairing heart tissues post myocardial infarction (MI).
31569016	9	93	theme	acute	1748:1752	arg1	models					1757:1762	acute MI models	1748:1762	acute MI models	1748:1762	In vivo experiments with acute MI models demonstrated improved therapeutic efficacy of MSC delivery in r(GO/alginate) microgels, exhibiting significant decreases in the infarction area and the improvement of cardiac function.
31569016	8	94	dep	showed	1535:1540	arg1	compared					1587:1594	compared	1587:1594	compared	1587:1594	Furthermore, cardiomyocytes (CMs), co-cultured with the encapsulated MSCs in transwells with H2O2 treatment, showed higher cell viability and cardiac maturation compared to monolayer cultured CMs, likely due to ROS scavenging by the gels and positive paracrine signals from the encapsulated MSCs.
31569016	1	95	theme	MSC	173:175	arg1	transplantation					178:192	Mesenchymal stem cell (MSC) transplantation	150:192	Mesenchymal stem cell (MSC) transplantation	150:192	Mesenchymal stem cell (MSC) transplantation is promising for repairing heart tissues post myocardial infarction (MI).
31569016	10	96	theme	other	2186:2190	arg1	types					2209:2213	other therapeutic cell types	2186:2213	other therapeutic cell types	2186:2213	We believe that our novel MSC encapsulation system with GO, alginate, and mild reduction, which exhibits high cell protection capacity (e.g., anti-oxidant activity), will serve as an effective platform for the delivery of stem cells and other therapeutic cell types to treat various injuries and diseases, including MI.
31569016	10	97	theme	mild	2023:2026	arg1	reduction					2028:2036	mild reduction	2023:2036	mild reduction	2023:2036	We believe that our novel MSC encapsulation system with GO, alginate, and mild reduction, which exhibits high cell protection capacity (e.g., anti-oxidant activity), will serve as an effective platform for the delivery of stem cells and other therapeutic cell types to treat various injuries and diseases, including MI.
31569016	5	98	theme	/alginate	966:974	arg1	microgels					986:994	graphene oxide (GO)/alginate composite microgels	947:994	graphene oxide (GO)/alginate composite microgels	947:994	Specifically, we encapsulated MSCs in graphene oxide (GO)/alginate composite microgels by electrospraying.
31569016	9	99	theme	improved	1777:1784	arg1	efficacy					1798:1805	improved therapeutic efficacy	1777:1805	improved therapeutic efficacy of MSC delivery in r(GO/alginate) microgels	1777:1849	In vivo experiments with acute MI models demonstrated improved therapeutic efficacy of MSC delivery in r(GO/alginate) microgels, exhibiting significant decreases in the infarction area and the improvement of cardiac function.
31569016	10	100	theme	cells	2176:2180	arg1	delivery					2159:2166	the delivery	2155:2166	the delivery of stem cells and other therapeutic cell types	2155:2213	We believe that our novel MSC encapsulation system with GO, alginate, and mild reduction, which exhibits high cell protection capacity (e.g., anti-oxidant activity), will serve as an effective platform for the delivery of stem cells and other therapeutic cell types to treat various injuries and diseases, including MI.
31569016	0	101	theme	infarcted	132:140	arg1	hearts					142:147	infarcted hearts	132:147	infarcted hearts	132:147	Anti-oxidant activity reinforced reduced graphene oxide/alginate microgels: Mesenchymal stem cell encapsulation and regeneration of infarcted hearts.
31569016	7	102	theme	r	1329:1329	arg1	microgels					1344:1352	the r(GO/alginate) microgels	1325:1352	the r(GO/alginate) microgels	1325:1352	Our in vitro studies demonstrated that the MSCs encapsulated in the r(GO/alginate) microgels showed increased viability under oxidative stress conditions with H2O2.
31569016	8	103	with	transwells	1503:1512	arg1	treatment					1524:1532	H2O2 treatment	1519:1532	H2O2 treatment	1519:1532	Furthermore, cardiomyocytes (CMs), co-cultured with the encapsulated MSCs in transwells with H2O2 treatment, showed higher cell viability and cardiac maturation compared to monolayer cultured CMs, likely due to ROS scavenging by the gels and positive paracrine signals from the encapsulated MSCs.
31569016	5	104	theme	oxide	956:960	arg1	microgels					986:994	graphene oxide (GO)/alginate composite microgels	947:994	graphene oxide (GO)/alginate composite microgels	947:994	Specifically, we encapsulated MSCs in graphene oxide (GO)/alginate composite microgels by electrospraying.
31569016	9	105	theme	MSC	1810:1812	arg1	delivery					1814:1821	MSC delivery	1810:1821	MSC delivery	1810:1821	In vivo experiments with acute MI models demonstrated improved therapeutic efficacy of MSC delivery in r(GO/alginate) microgels, exhibiting significant decreases in the infarction area and the improvement of cardiac function.
31569016	8	106	theme	cell	1549:1552	arg1	viability					1554:1562	higher cell viability	1542:1562	higher cell viability	1542:1562	Furthermore, cardiomyocytes (CMs), co-cultured with the encapsulated MSCs in transwells with H2O2 treatment, showed higher cell viability and cardiac maturation compared to monolayer cultured CMs, likely due to ROS scavenging by the gels and positive paracrine signals from the encapsulated MSCs.
31569016	10	107	theme	cell	2204:2207	arg1	types					2209:2213	other therapeutic cell types	2186:2213	other therapeutic cell types	2186:2213	We believe that our novel MSC encapsulation system with GO, alginate, and mild reduction, which exhibits high cell protection capacity (e.g., anti-oxidant activity), will serve as an effective platform for the delivery of stem cells and other therapeutic cell types to treat various injuries and diseases, including MI.
31569016	0	108	theme	reduced	33:39	arg1	cell					93:96	Mesenchymal stem cell encapsulation and regeneration	76:127	cell	93:96	Anti-oxidant activity reinforced reduced graphene oxide/alginate microgels: Mesenchymal stem cell encapsulation and regeneration of infarcted hearts.
31569016	0	108	theme	reduced	33:39	arg1	regeneration					116:127	regeneration	116:127	regeneration	116:127	Anti-oxidant activity reinforced reduced graphene oxide/alginate microgels: Mesenchymal stem cell encapsulation and regeneration of infarcted hearts.
31569016	0	108	theme	reduced	33:39	arg1	microgels					65:73	reduced graphene oxide/alginate microgels	33:73	reduced graphene oxide/alginate microgels: Mesenchymal stem cell encapsulation and regeneration of infarcted hearts	33:147	Anti-oxidant activity reinforced reduced graphene oxide/alginate microgels: Mesenchymal stem cell encapsulation and regeneration of infarcted hearts.
31569016	5	109	from	MSCs	939:942	arg1	microgels					986:994	graphene oxide (GO)/alginate composite microgels	947:994	graphene oxide (GO)/alginate composite microgels	947:994	Specifically, we encapsulated MSCs in graphene oxide (GO)/alginate composite microgels by electrospraying.
31569016	3	110	theme	shear	661:665	arg1	stress					667:672	high shear stress	656:672	high shear stress	656:672	Nevertheless, its therapeutic efficacy still remains low, which is strongly associated with low viability and activity of the transplanted stem cells, because the transplanted MSCs are exposed to high shear stress during injection and harsh environments (e.g., high oxidative stress and host immune reactions) post injection.
31569016	0	111	theme	oxide/alginate	50:63	arg1	cell					93:96	Mesenchymal stem cell encapsulation and regeneration	76:127	cell	93:96	Anti-oxidant activity reinforced reduced graphene oxide/alginate microgels: Mesenchymal stem cell encapsulation and regeneration of infarcted hearts.
31569016	0	111	theme	oxide/alginate	50:63	arg1	regeneration					116:127	regeneration	116:127	regeneration	116:127	Anti-oxidant activity reinforced reduced graphene oxide/alginate microgels: Mesenchymal stem cell encapsulation and regeneration of infarcted hearts.
31569016	0	111	theme	oxide/alginate	50:63	arg1	microgels					65:73	reduced graphene oxide/alginate microgels	33:73	reduced graphene oxide/alginate microgels: Mesenchymal stem cell encapsulation and regeneration of infarcted hearts	33:147	Anti-oxidant activity reinforced reduced graphene oxide/alginate microgels: Mesenchymal stem cell encapsulation and regeneration of infarcted hearts.
31569016	9	112	from	efficacy	1798:1805	arg1	microgels					1841:1849	r(GO/alginate) microgels	1826:1849	r(GO/alginate) microgels	1826:1849	In vivo experiments with acute MI models demonstrated improved therapeutic efficacy of MSC delivery in r(GO/alginate) microgels, exhibiting significant decreases in the infarction area and the improvement of cardiac function.
31569016	6	113	theme	MSC-embedded	1099:1110	arg1	microgels					1124:1132	reduced MSC-embedded GO/alginate microgels	1091:1132	reduced MSC-embedded GO/alginate microgels (i.e., r(GO/alginate))	1091:1155	To further enhance the anti-oxidizing activities of the gels, we developed reduced MSC-embedded GO/alginate microgels (i.e., r(GO/alginate)), which have the potential to protect MSCs from the abovementioned harsh environments within MI tissues.
31569016	2	114	theme	growth	412:417	arg1	factors					419:425	multiple growth factors	403:425	multiple growth factors	403:425	In particular, paracrine effects of the transplanted MSCs have been highlighted to play major roles in heart regeneration by secreting multiple growth factors and immune-modulatory cytokines.
31569016	9	115	with	experiments	1731:1741	arg1	models					1757:1762	acute MI models	1748:1762	acute MI models	1748:1762	In vivo experiments with acute MI models demonstrated improved therapeutic efficacy of MSC delivery in r(GO/alginate) microgels, exhibiting significant decreases in the infarction area and the improvement of cardiac function.
31569016	7	116	theme	in	1265:1266	arg1	studies					1274:1280	Our in vitro studies	1261:1280	Our in vitro studies	1261:1280	Our in vitro studies demonstrated that the MSCs encapsulated in the r(GO/alginate) microgels showed increased viability under oxidative stress conditions with H2O2.
31569016	6	117	contain	have	1164:1167	arg2	potential					1173:1181	the potential to protect MSCs from the abovementioned harsh environments within MI tissues	1169:1258	the potential to protect MSCs from the abovementioned harsh environments within MI tissues	1169:1258	To further enhance the anti-oxidizing activities of the gels, we developed reduced MSC-embedded GO/alginate microgels (i.e., r(GO/alginate)), which have the potential to protect MSCs from the abovementioned harsh environments within MI tissues.
31569016	6	117	contain	have	1164:1167	arg1	microgels					1124:1132	reduced MSC-embedded GO/alginate microgels	1091:1132	reduced MSC-embedded GO/alginate microgels (i.e., r(GO/alginate))	1091:1155	To further enhance the anti-oxidizing activities of the gels, we developed reduced MSC-embedded GO/alginate microgels (i.e., r(GO/alginate)), which have the potential to protect MSCs from the abovementioned harsh environments within MI tissues.
31569016	9	118	theme	significant	1863:1873	arg1	decreases					1875:1883	significant decreases	1863:1883	significant decreases in the infarction area	1863:1906	In vivo experiments with acute MI models demonstrated improved therapeutic efficacy of MSC delivery in r(GO/alginate) microgels, exhibiting significant decreases in the infarction area and the improvement of cardiac function.
31569016	8	119	theme	positive	1668:1675	arg1	signals					1687:1693	positive paracrine signals	1668:1693	positive paracrine signals	1668:1693	Furthermore, cardiomyocytes (CMs), co-cultured with the encapsulated MSCs in transwells with H2O2 treatment, showed higher cell viability and cardiac maturation compared to monolayer cultured CMs, likely due to ROS scavenging by the gels and positive paracrine signals from the encapsulated MSCs.
31402073	8	0	theme	phylogenetic	928:939	arg1	pMGs					955:958	pMGs	955:958	pMGs	955:958	Phylogenetic analyses based on 16S rRNA gene sequences and single-copy phylogenetic marker genes (pMGs) showed that the two strains belonged to the genus Labedella and formed a separate cluster from the closest species L. gwakjiensis KCTC 19176T and L. endophytica CPCC 203961T.
31402073	8	0	theme	phylogenetic	928:939	arg1	genes					948:952	single-copy phylogenetic marker genes	916:952	single-copy phylogenetic marker genes (pMGs)	916:959	Phylogenetic analyses based on 16S rRNA gene sequences and single-copy phylogenetic marker genes (pMGs) showed that the two strains belonged to the genus Labedella and formed a separate cluster from the closest species L. gwakjiensis KCTC 19176T and L. endophytica CPCC 203961T.
31402073	2	1	theme	emended	141:147	arg1	description					149:159	emended description	141:159	emended description of the genus Labedella	141:182	nov., two endophytic actinobacteria isolated from plants in the Taklamakan Desert and emended description of the genus Labedella.
31402073	10	2	theme	genus	1515:1519	arg1	Labedella					1521:1529	the genus Labedella	1511:1529	the genus Labedella	1511:1529	The two strains showed chemotaxonomic characteristics and phenotypic properties in agreement with the description of the genus Labedella and also confirmed the differentiation from the closest species.
31402073	9	3	theme	species	1373:1379	arg1	delineation					1381:1391	species delineation	1373:1391	species delineation	1373:1391	Genomic analyses, including average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH), clearly separated the strains from each other and from the other species of the genus Labedella with values below the thresholds for species delineation.
31402073	2	4	from	plants	105:110	arg1	Desert					130:135	the Taklamakan Desert	115:135	the Taklamakan Desert	115:135	nov., two endophytic actinobacteria isolated from plants in the Taklamakan Desert and emended description of the genus Labedella.
31402073	10	5	from	species	1587:1593	arg1	differentiation					1554:1568	the differentiation	1550:1568	the differentiation from the closest species	1550:1593	The two strains showed chemotaxonomic characteristics and phenotypic properties in agreement with the description of the genus Labedella and also confirmed the differentiation from the closest species.
31402073	8	6	dep	species	1068:1074	arg1	19176T					1096:1101	L. gwakjiensis KCTC 19176T	1076:1101	L. gwakjiensis KCTC 19176T	1076:1101	Phylogenetic analyses based on 16S rRNA gene sequences and single-copy phylogenetic marker genes (pMGs) showed that the two strains belonged to the genus Labedella and formed a separate cluster from the closest species L. gwakjiensis KCTC 19176T and L. endophytica CPCC 203961T.
31402073	8	6	dep	species	1068:1074	arg1	species					1068:1074	the closest species L. gwakjiensis KCTC 19176T and L. endophytica CPCC 203961T	1056:1133	the closest species L. gwakjiensis KCTC 19176T and L. endophytica CPCC 203961T	1056:1133	Phylogenetic analyses based on 16S rRNA gene sequences and single-copy phylogenetic marker genes (pMGs) showed that the two strains belonged to the genus Labedella and formed a separate cluster from the closest species L. gwakjiensis KCTC 19176T and L. endophytica CPCC 203961T.
31402073	8	6	dep	species	1068:1074	arg1	203961T					1127:1133	L. endophytica CPCC 203961T	1107:1133	L. endophytica CPCC 203961T	1107:1133	Phylogenetic analyses based on 16S rRNA gene sequences and single-copy phylogenetic marker genes (pMGs) showed that the two strains belonged to the genus Labedella and formed a separate cluster from the closest species L. gwakjiensis KCTC 19176T and L. endophytica CPCC 203961T.
31402073	9	7	theme	nucleotide	1172:1181	arg1	ANI					1193:1195	ANI	1193:1195	ANI	1193:1195	Genomic analyses, including average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH), clearly separated the strains from each other and from the other species of the genus Labedella with values below the thresholds for species delineation.
31402073	9	7	theme	nucleotide	1172:1181	arg1	identity					1183:1190	average nucleotide identity	1164:1190	average nucleotide identity (ANI)	1164:1196	Genomic analyses, including average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH), clearly separated the strains from each other and from the other species of the genus Labedella with values below the thresholds for species delineation.
31402073	3	8	theme	surface-sterilized	260:277	arg1	tissues					285:291	surface-sterilized plant tissues	260:291	surface-sterilized plant tissues collected from the Taklamakan Desert in the Xinjiang Uygur Autonomous Region, China	260:375	Two novel strains, designated 11W25H-1T and 8H24J-4-2T, were isolated from surface-sterilized plant tissues collected from the Taklamakan Desert in the Xinjiang Uygur Autonomous Region, China.
31402073	6	9	theme	rRNA	575:578	arg1	sequences					585:593	The 16S rRNA gene sequences	567:593	The 16S rRNA gene sequences of the strains	567:608	The 16S rRNA gene sequences of the strains showed highest similarities with Labedella gwakjiensis KCTC 19176T (99.2% and 98.9%, respectively) and Labedella endophytica CPCC 203961T (98.9% and 99.0%, respectively).
31402073	2	10	theme	endophytic	65:74	arg1	nov.					55:58	nov.	55:58	nov.	55:58	nov., two endophytic actinobacteria isolated from plants in the Taklamakan Desert and emended description of the genus Labedella.
31402073	2	10	theme	endophytic	65:74	arg1	actinobacteria					76:89	two endophytic actinobacteria	61:89	two endophytic actinobacteria	61:89	nov., two endophytic actinobacteria isolated from plants in the Taklamakan Desert and emended description of the genus Labedella.
31402073	9	11	theme	digital	1202:1208	arg1	dDDH					1233:1236	dDDH	1233:1236	dDDH	1233:1236	Genomic analyses, including average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH), clearly separated the strains from each other and from the other species of the genus Labedella with values below the thresholds for species delineation.
31402073	9	11	theme	digital	1202:1208	arg1	hybridization					1218:1230	digital DNA-DNA hybridization	1202:1230	digital DNA-DNA hybridization (dDDH)	1202:1237	Genomic analyses, including average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH), clearly separated the strains from each other and from the other species of the genus Labedella with values below the thresholds for species delineation.
31402073	9	12	theme	DNA-DNA	1210:1216	arg1	dDDH					1233:1236	dDDH	1233:1236	dDDH	1233:1236	Genomic analyses, including average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH), clearly separated the strains from each other and from the other species of the genus Labedella with values below the thresholds for species delineation.
31402073	9	12	theme	DNA-DNA	1210:1216	arg1	hybridization					1218:1230	digital DNA-DNA hybridization	1202:1230	digital DNA-DNA hybridization (dDDH)	1202:1237	Genomic analyses, including average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH), clearly separated the strains from each other and from the other species of the genus Labedella with values below the thresholds for species delineation.
31402073	5	13	dep	Gram-stain	491:500	arg1	positive					502:509	positive	502:509	positive	502:509	They were Gram-stain positive, aerobic, non-motile, non-spore-forming and rod-shaped.
31402073	1	14	dep	sp	51:52	arg1	populi					44:49	nov. and Labedella populi	25:49	nov. and Labedella populi	25:49	nov. and Labedella populi sp.
31402073	6	15	theme	KCTC	665:668	arg1	19176T					670:675	Labedella gwakjiensis KCTC 19176T	643:675	Labedella gwakjiensis KCTC 19176T (99.2% and 98.9%, respectively)	643:707	The 16S rRNA gene sequences of the strains showed highest similarities with Labedella gwakjiensis KCTC 19176T (99.2% and 98.9%, respectively) and Labedella endophytica CPCC 203961T (98.9% and 99.0%, respectively).
31402073	6	16	dep	Labedella	643:651	arg1	gwakjiensis					653:663	gwakjiensis	653:663	gwakjiensis	653:663	The 16S rRNA gene sequences of the strains showed highest similarities with Labedella gwakjiensis KCTC 19176T (99.2% and 98.9%, respectively) and Labedella endophytica CPCC 203961T (98.9% and 99.0%, respectively).
31402073	6	17	theme	Labedella	643:651	arg1	19176T					670:675	Labedella gwakjiensis KCTC 19176T	643:675	Labedella gwakjiensis KCTC 19176T (99.2% and 98.9%, respectively)	643:707	The 16S rRNA gene sequences of the strains showed highest similarities with Labedella gwakjiensis KCTC 19176T (99.2% and 98.9%, respectively) and Labedella endophytica CPCC 203961T (98.9% and 99.0%, respectively).
31402073	3	18	theme	Taklamakan	312:321	arg1	Desert					323:328	the Taklamakan Desert	308:328	the Taklamakan Desert	308:328	Two novel strains, designated 11W25H-1T and 8H24J-4-2T, were isolated from surface-sterilized plant tissues collected from the Taklamakan Desert in the Xinjiang Uygur Autonomous Region, China.
31402073	8	19	from	species	1068:1074	arg1	cluster					1043:1049	a separate cluster	1032:1049	a separate cluster from the closest species L. gwakjiensis KCTC 19176T and L. endophytica CPCC 203961T	1032:1133	Phylogenetic analyses based on 16S rRNA gene sequences and single-copy phylogenetic marker genes (pMGs) showed that the two strains belonged to the genus Labedella and formed a separate cluster from the closest species L. gwakjiensis KCTC 19176T and L. endophytica CPCC 203961T.
31402073	13	20	theme	strain	1846:1851	arg1	1.16697T					1881:1888	type strain 8H24J-4-2T=JCM 33143T=CGMCC 1.16697T	1841:1888	type strain 8H24J-4-2T=JCM 33143T=CGMCC 1.16697T	1841:1888	nov. (type strain 8H24J-4-2T=JCM 33143T=CGMCC 1.16697T) are proposed.
31402073	13	20	theme	strain	1846:1851	arg1	nov					1835:1837	nov	1835:1837	nov	1835:1837	nov. (type strain 8H24J-4-2T=JCM 33143T=CGMCC 1.16697T) are proposed.
31402073	4	21	theme	taxonomic	460:468	arg1	positions					470:478	their taxonomic positions	454:478	their taxonomic positions	454:478	The strains were characterized by a polyphasic approach in order to clarify their taxonomic positions.
31402073	8	22	theme	genus	1005:1009	arg1	Labedella					1011:1019	the genus Labedella	1001:1019	the genus Labedella	1001:1019	Phylogenetic analyses based on 16S rRNA gene sequences and single-copy phylogenetic marker genes (pMGs) showed that the two strains belonged to the genus Labedella and formed a separate cluster from the closest species L. gwakjiensis KCTC 19176T and L. endophytica CPCC 203961T.
31402073	10	23	theme	Labedella	1521:1529	arg1	description					1496:1506	the description	1492:1506	the description of the genus Labedella	1492:1529	The two strains showed chemotaxonomic characteristics and phenotypic properties in agreement with the description of the genus Labedella and also confirmed the differentiation from the closest species.
31402073	3	24	theme	novel	189:193	arg1	strains					195:201	Two novel strains	185:201	Two novel strains	185:201	Two novel strains, designated 11W25H-1T and 8H24J-4-2T, were isolated from surface-sterilized plant tissues collected from the Taklamakan Desert in the Xinjiang Uygur Autonomous Region, China.
31402073	0	25	theme	Labedella	0:8	arg1	sp					21:22	Labedella phragmitis sp	0:22	Labedella phragmitis sp.	0:23	Labedella phragmitis sp.
31402073	9	26	from	other	1280:1284	arg1	strains					1262:1268	the strains	1258:1268	the strains from each other and from the other species of the genus Labedella with values below the thresholds for species delineation	1258:1391	Genomic analyses, including average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH), clearly separated the strains from each other and from the other species of the genus Labedella with values below the thresholds for species delineation.
31402073	10	27	theme	phenotypic	1452:1461	arg1	properties					1463:1472	phenotypic properties	1452:1472	phenotypic properties	1452:1472	The two strains showed chemotaxonomic characteristics and phenotypic properties in agreement with the description of the genus Labedella and also confirmed the differentiation from the closest species.
31402073	2	28	theme	genus	168:172	arg1	Labedella					174:182	the genus Labedella	164:182	the genus Labedella	164:182	nov., two endophytic actinobacteria isolated from plants in the Taklamakan Desert and emended description of the genus Labedella.
31402073	11	29	dep	strains	1623:1629	arg1	11W25H-1T					1631:1639	11W25H-1T	1631:1639	11W25H-1T	1631:1639	The data demonstrated that strains 11W25H-1T and 8H24J-4-2T represented two novel species of the genus Labedella, for which the names Labedella phragmitis sp.
31402073	11	29	dep	strains	1623:1629	arg1	strains					1623:1629	strains	1623:1629	strains 11W25H-1T and 8H24J-4-2T	1623:1654	The data demonstrated that strains 11W25H-1T and 8H24J-4-2T represented two novel species of the genus Labedella, for which the names Labedella phragmitis sp.
31402073	11	29	dep	strains	1623:1629	arg1	8H24J-4-2T					1645:1654	8H24J-4-2T	1645:1654	8H24J-4-2T	1645:1654	The data demonstrated that strains 11W25H-1T and 8H24J-4-2T represented two novel species of the genus Labedella, for which the names Labedella phragmitis sp.
31402073	12	30	theme	strain	1766:1771	arg1	1.16700T					1800:1807	type strain 11W25H-1T=JCM 33144T=CGMCC 1.16700T	1761:1807	type strain 11W25H-1T=JCM 33144T=CGMCC 1.16700T	1761:1807	nov. (type strain 11W25H-1T=JCM 33144T=CGMCC 1.16700T) and Labedella populi sp.
31402073	12	30	theme	strain	1766:1771	arg1	nov					1755:1757	nov	1755:1757	nov	1755:1757	nov. (type strain 11W25H-1T=JCM 33144T=CGMCC 1.16700T) and Labedella populi sp.
31402073	12	31	theme	33144T=CGMCC	1787:1798	arg1	1.16700T					1800:1807	type strain 11W25H-1T=JCM 33144T=CGMCC 1.16700T	1761:1807	type strain 11W25H-1T=JCM 33144T=CGMCC 1.16700T	1761:1807	nov. (type strain 11W25H-1T=JCM 33144T=CGMCC 1.16700T) and Labedella populi sp.
31402073	12	31	theme	33144T=CGMCC	1787:1798	arg1	nov					1755:1757	nov	1755:1757	nov	1755:1757	nov. (type strain 11W25H-1T=JCM 33144T=CGMCC 1.16700T) and Labedella populi sp.
31402073	3	32	attach	isolated	246:253	arg2	strains					195:201	Two novel strains	185:201	Two novel strains	185:201	Two novel strains, designated 11W25H-1T and 8H24J-4-2T, were isolated from surface-sterilized plant tissues collected from the Taklamakan Desert in the Xinjiang Uygur Autonomous Region, China.
31402073	3	32	attach	isolated	246:253	arg1	tissues					285:291	surface-sterilized plant tissues	260:291	surface-sterilized plant tissues collected from the Taklamakan Desert in the Xinjiang Uygur Autonomous Region, China	260:375	Two novel strains, designated 11W25H-1T and 8H24J-4-2T, were isolated from surface-sterilized plant tissues collected from the Taklamakan Desert in the Xinjiang Uygur Autonomous Region, China.
31402073	11	33	theme	Labedella	1699:1707	arg1	species					1678:1684	two novel species	1668:1684	two novel species	1668:1684	The data demonstrated that strains 11W25H-1T and 8H24J-4-2T represented two novel species of the genus Labedella, for which the names Labedella phragmitis sp.
31402073	6	34	theme	CPCC	735:738	arg1	203961T					740:746	Labedella endophytica CPCC 203961T	713:746	Labedella endophytica CPCC 203961T (98.9% and 99.0%, respectively)	713:778	The 16S rRNA gene sequences of the strains showed highest similarities with Labedella gwakjiensis KCTC 19176T (99.2% and 98.9%, respectively) and Labedella endophytica CPCC 203961T (98.9% and 99.0%, respectively).
31402073	8	35	theme	rRNA	892:895	arg1	sequences					902:910	16S rRNA gene sequences	888:910	16S rRNA gene sequences	888:910	Phylogenetic analyses based on 16S rRNA gene sequences and single-copy phylogenetic marker genes (pMGs) showed that the two strains belonged to the genus Labedella and formed a separate cluster from the closest species L. gwakjiensis KCTC 19176T and L. endophytica CPCC 203961T.
31402073	8	36	theme	single-copy	916:926	arg1	pMGs					955:958	pMGs	955:958	pMGs	955:958	Phylogenetic analyses based on 16S rRNA gene sequences and single-copy phylogenetic marker genes (pMGs) showed that the two strains belonged to the genus Labedella and formed a separate cluster from the closest species L. gwakjiensis KCTC 19176T and L. endophytica CPCC 203961T.
31402073	8	36	theme	single-copy	916:926	arg1	genes					948:952	single-copy phylogenetic marker genes	916:952	single-copy phylogenetic marker genes (pMGs)	916:959	Phylogenetic analyses based on 16S rRNA gene sequences and single-copy phylogenetic marker genes (pMGs) showed that the two strains belonged to the genus Labedella and formed a separate cluster from the closest species L. gwakjiensis KCTC 19176T and L. endophytica CPCC 203961T.
31402073	2	37	theme	Labedella	174:182	arg1	description					149:159	emended description	141:159	emended description of the genus Labedella	141:182	nov., two endophytic actinobacteria isolated from plants in the Taklamakan Desert and emended description of the genus Labedella.
31402073	2	37	theme	Labedella	174:182	arg1	plants					105:110	plants	105:110	plants in the Taklamakan Desert	105:135	nov., two endophytic actinobacteria isolated from plants in the Taklamakan Desert and emended description of the genus Labedella.
31402073	6	38	theme	Labedella	713:721	arg1	203961T					740:746	Labedella endophytica CPCC 203961T	713:746	Labedella endophytica CPCC 203961T (98.9% and 99.0%, respectively)	713:778	The 16S rRNA gene sequences of the strains showed highest similarities with Labedella gwakjiensis KCTC 19176T (99.2% and 98.9%, respectively) and Labedella endophytica CPCC 203961T (98.9% and 99.0%, respectively).
31402073	9	39	theme	Labedella	1326:1334	arg1	species					1305:1311	the other species	1295:1311	the other species of the genus Labedella	1295:1334	Genomic analyses, including average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH), clearly separated the strains from each other and from the other species of the genus Labedella with values below the thresholds for species delineation.
31402073	2	40	from	description	149:159	arg1	Desert					130:135	the Taklamakan Desert	115:135	the Taklamakan Desert	115:135	nov., two endophytic actinobacteria isolated from plants in the Taklamakan Desert and emended description of the genus Labedella.
31402073	8	41	theme	marker	941:946	arg1	pMGs					955:958	pMGs	955:958	pMGs	955:958	Phylogenetic analyses based on 16S rRNA gene sequences and single-copy phylogenetic marker genes (pMGs) showed that the two strains belonged to the genus Labedella and formed a separate cluster from the closest species L. gwakjiensis KCTC 19176T and L. endophytica CPCC 203961T.
31402073	8	41	theme	marker	941:946	arg1	genes					948:952	single-copy phylogenetic marker genes	916:952	single-copy phylogenetic marker genes (pMGs)	916:959	Phylogenetic analyses based on 16S rRNA gene sequences and single-copy phylogenetic marker genes (pMGs) showed that the two strains belonged to the genus Labedella and formed a separate cluster from the closest species L. gwakjiensis KCTC 19176T and L. endophytica CPCC 203961T.
31402073	6	42	theme	strains	602:608	arg1	sequences					585:593	The 16S rRNA gene sequences	567:593	The 16S rRNA gene sequences of the strains	567:608	The 16S rRNA gene sequences of the strains showed highest similarities with Labedella gwakjiensis KCTC 19176T (99.2% and 98.9%, respectively) and Labedella endophytica CPCC 203961T (98.9% and 99.0%, respectively).
31402073	6	43	dep	203961T	740:746	arg1	%					753:753	98.9%	749:753	98.9%	749:753	The 16S rRNA gene sequences of the strains showed highest similarities with Labedella gwakjiensis KCTC 19176T (99.2% and 98.9%, respectively) and Labedella endophytica CPCC 203961T (98.9% and 99.0%, respectively).
31402073	6	43	dep	203961T	740:746	arg1	%					763:763	99.0%	759:763	99.0%	759:763	The 16S rRNA gene sequences of the strains showed highest similarities with Labedella gwakjiensis KCTC 19176T (99.2% and 98.9%, respectively) and Labedella endophytica CPCC 203961T (98.9% and 99.0%, respectively).
31402073	9	44	theme	Genomic	1136:1142	arg1	analyses					1144:1151	Genomic analyses	1136:1151	Genomic analyses	1136:1151	Genomic analyses, including average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH), clearly separated the strains from each other and from the other species of the genus Labedella with values below the thresholds for species delineation.
31402073	9	44	theme	Genomic	1136:1142	arg1	hybridization					1218:1230	digital DNA-DNA hybridization	1202:1230	digital DNA-DNA hybridization (dDDH)	1202:1237	Genomic analyses, including average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH), clearly separated the strains from each other and from the other species of the genus Labedella with values below the thresholds for species delineation.
31402073	9	44	theme	Genomic	1136:1142	arg1	identity					1183:1190	average nucleotide identity	1164:1190	average nucleotide identity (ANI)	1164:1196	Genomic analyses, including average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH), clearly separated the strains from each other and from the other species of the genus Labedella with values below the thresholds for species delineation.
31402073	8	45	theme	L.	1107:1108	arg1	species					1068:1074	the closest species L. gwakjiensis KCTC 19176T and L. endophytica CPCC 203961T	1056:1133	the closest species L. gwakjiensis KCTC 19176T and L. endophytica CPCC 203961T	1056:1133	Phylogenetic analyses based on 16S rRNA gene sequences and single-copy phylogenetic marker genes (pMGs) showed that the two strains belonged to the genus Labedella and formed a separate cluster from the closest species L. gwakjiensis KCTC 19176T and L. endophytica CPCC 203961T.
31402073	8	45	theme	L.	1107:1108	arg1	203961T					1127:1133	L. endophytica CPCC 203961T	1107:1133	L. endophytica CPCC 203961T	1107:1133	Phylogenetic analyses based on 16S rRNA gene sequences and single-copy phylogenetic marker genes (pMGs) showed that the two strains belonged to the genus Labedella and formed a separate cluster from the closest species L. gwakjiensis KCTC 19176T and L. endophytica CPCC 203961T.
31402073	8	46	theme	16S	888:890	arg1	sequences					902:910	16S rRNA gene sequences	888:910	16S rRNA gene sequences	888:910	Phylogenetic analyses based on 16S rRNA gene sequences and single-copy phylogenetic marker genes (pMGs) showed that the two strains belonged to the genus Labedella and formed a separate cluster from the closest species L. gwakjiensis KCTC 19176T and L. endophytica CPCC 203961T.
31402073	12	47	dep	sp	1831:1832	arg1	1.16700T					1800:1807	type strain 11W25H-1T=JCM 33144T=CGMCC 1.16700T	1761:1807	type strain 11W25H-1T=JCM 33144T=CGMCC 1.16700T	1761:1807	nov. (type strain 11W25H-1T=JCM 33144T=CGMCC 1.16700T) and Labedella populi sp.
31402073	12	47	dep	sp	1831:1832	arg1	Labedella					1814:1822	Labedella	1814:1822	Labedella	1814:1822	nov. (type strain 11W25H-1T=JCM 33144T=CGMCC 1.16700T) and Labedella populi sp.
31402073	12	47	dep	sp	1831:1832	arg1	nov					1755:1757	nov	1755:1757	nov	1755:1757	nov. (type strain 11W25H-1T=JCM 33144T=CGMCC 1.16700T) and Labedella populi sp.
31402073	8	48	theme	CPCC	1122:1125	arg1	species					1068:1074	the closest species L. gwakjiensis KCTC 19176T and L. endophytica CPCC 203961T	1056:1133	the closest species L. gwakjiensis KCTC 19176T and L. endophytica CPCC 203961T	1056:1133	Phylogenetic analyses based on 16S rRNA gene sequences and single-copy phylogenetic marker genes (pMGs) showed that the two strains belonged to the genus Labedella and formed a separate cluster from the closest species L. gwakjiensis KCTC 19176T and L. endophytica CPCC 203961T.
31402073	8	48	theme	CPCC	1122:1125	arg1	203961T					1127:1133	L. endophytica CPCC 203961T	1107:1133	L. endophytica CPCC 203961T	1107:1133	Phylogenetic analyses based on 16S rRNA gene sequences and single-copy phylogenetic marker genes (pMGs) showed that the two strains belonged to the genus Labedella and formed a separate cluster from the closest species L. gwakjiensis KCTC 19176T and L. endophytica CPCC 203961T.
31402073	8	49	dep	L.	1076:1077	arg1	gwakjiensis					1079:1089	gwakjiensis	1079:1089	gwakjiensis	1079:1089	Phylogenetic analyses based on 16S rRNA gene sequences and single-copy phylogenetic marker genes (pMGs) showed that the two strains belonged to the genus Labedella and formed a separate cluster from the closest species L. gwakjiensis KCTC 19176T and L. endophytica CPCC 203961T.
31402073	2	50	theme	Taklamakan	119:128	arg1	Desert					130:135	the Taklamakan Desert	115:135	the Taklamakan Desert	115:135	nov., two endophytic actinobacteria isolated from plants in the Taklamakan Desert and emended description of the genus Labedella.
31402073	9	51	theme	average	1164:1170	arg1	ANI					1193:1195	ANI	1193:1195	ANI	1193:1195	Genomic analyses, including average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH), clearly separated the strains from each other and from the other species of the genus Labedella with values below the thresholds for species delineation.
31402073	9	51	theme	average	1164:1170	arg1	identity					1183:1190	average nucleotide identity	1164:1190	average nucleotide identity (ANI)	1164:1196	Genomic analyses, including average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH), clearly separated the strains from each other and from the other species of the genus Labedella with values below the thresholds for species delineation.
31402073	13	52	theme	33143T=CGMCC	1868:1879	arg1	1.16697T					1881:1888	type strain 8H24J-4-2T=JCM 33143T=CGMCC 1.16697T	1841:1888	type strain 8H24J-4-2T=JCM 33143T=CGMCC 1.16697T	1841:1888	nov. (type strain 8H24J-4-2T=JCM 33143T=CGMCC 1.16697T) are proposed.
31402073	13	52	theme	33143T=CGMCC	1868:1879	arg1	nov					1835:1837	nov	1835:1837	nov	1835:1837	nov. (type strain 8H24J-4-2T=JCM 33143T=CGMCC 1.16697T) are proposed.
31402073	3	53	theme	plant	279:283	arg1	tissues					285:291	surface-sterilized plant tissues	260:291	surface-sterilized plant tissues collected from the Taklamakan Desert in the Xinjiang Uygur Autonomous Region, China	260:375	Two novel strains, designated 11W25H-1T and 8H24J-4-2T, were isolated from surface-sterilized plant tissues collected from the Taklamakan Desert in the Xinjiang Uygur Autonomous Region, China.
31402073	6	54	theme	gene	580:583	arg1	sequences					585:593	The 16S rRNA gene sequences	567:593	The 16S rRNA gene sequences of the strains	567:608	The 16S rRNA gene sequences of the strains showed highest similarities with Labedella gwakjiensis KCTC 19176T (99.2% and 98.9%, respectively) and Labedella endophytica CPCC 203961T (98.9% and 99.0%, respectively).
31402073	13	55	theme	type	1841:1844	arg1	1.16697T					1881:1888	type strain 8H24J-4-2T=JCM 33143T=CGMCC 1.16697T	1841:1888	type strain 8H24J-4-2T=JCM 33143T=CGMCC 1.16697T	1841:1888	nov. (type strain 8H24J-4-2T=JCM 33143T=CGMCC 1.16697T) are proposed.
31402073	13	55	theme	type	1841:1844	arg1	nov					1835:1837	nov	1835:1837	nov	1835:1837	nov. (type strain 8H24J-4-2T=JCM 33143T=CGMCC 1.16697T) are proposed.
31402073	13	56	theme	8H24J-4-2T=JCM	1853:1866	arg1	1.16697T					1881:1888	type strain 8H24J-4-2T=JCM 33143T=CGMCC 1.16697T	1841:1888	type strain 8H24J-4-2T=JCM 33143T=CGMCC 1.16697T	1841:1888	nov. (type strain 8H24J-4-2T=JCM 33143T=CGMCC 1.16697T) are proposed.
31402073	13	56	theme	8H24J-4-2T=JCM	1853:1866	arg1	nov					1835:1837	nov	1835:1837	nov	1835:1837	nov. (type strain 8H24J-4-2T=JCM 33143T=CGMCC 1.16697T) are proposed.
31402073	7	57	theme	sequence	785:792	arg1	similarity					794:803	The sequence similarity	781:803	The sequence similarity between strains 11W25H-1T and 8H24J-4-2T	781:844	The sequence similarity between strains 11W25H-1T and 8H24J-4-2T was 99.4%.
31402073	7	57	theme	sequence	785:792	arg1	%					854:854	99.4%	850:854	99.4%	850:854	The sequence similarity between strains 11W25H-1T and 8H24J-4-2T was 99.4%.
31402073	10	58	theme	closest	1579:1585	arg1	species					1587:1593	the closest species	1575:1593	the closest species	1575:1593	The two strains showed chemotaxonomic characteristics and phenotypic properties in agreement with the description of the genus Labedella and also confirmed the differentiation from the closest species.
31402073	4	59	theme	polyphasic	414:423	arg1	approach					425:432	a polyphasic approach	412:432	a polyphasic approach in order to clarify their taxonomic positions	412:478	The strains were characterized by a polyphasic approach in order to clarify their taxonomic positions.
31402073	9	60	with	strains	1262:1268	arg1	values					1341:1346	values	1341:1346	values below the thresholds for species delineation	1341:1391	Genomic analyses, including average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH), clearly separated the strains from each other and from the other species of the genus Labedella with values below the thresholds for species delineation.
31402073	11	61	dep	Labedella	1730:1738	arg1	phragmitis					1740:1749	phragmitis	1740:1749	phragmitis	1740:1749	The data demonstrated that strains 11W25H-1T and 8H24J-4-2T represented two novel species of the genus Labedella, for which the names Labedella phragmitis sp.
31402073	12	62	theme	11W25H-1T=JCM	1773:1785	arg1	1.16700T					1800:1807	type strain 11W25H-1T=JCM 33144T=CGMCC 1.16700T	1761:1807	type strain 11W25H-1T=JCM 33144T=CGMCC 1.16700T	1761:1807	nov. (type strain 11W25H-1T=JCM 33144T=CGMCC 1.16700T) and Labedella populi sp.
31402073	12	62	theme	11W25H-1T=JCM	1773:1785	arg1	nov					1755:1757	nov	1755:1757	nov	1755:1757	nov. (type strain 11W25H-1T=JCM 33144T=CGMCC 1.16700T) and Labedella populi sp.
31402073	11	63	dep	sp	1751:1752	arg1	names					1724:1728	the names	1720:1728	the names	1720:1728	The data demonstrated that strains 11W25H-1T and 8H24J-4-2T represented two novel species of the genus Labedella, for which the names Labedella phragmitis sp.
31402073	6	64	dep	19176T	670:675	arg1	%					682:682	99.2%	678:682	99.2%	678:682	The 16S rRNA gene sequences of the strains showed highest similarities with Labedella gwakjiensis KCTC 19176T (99.2% and 98.9%, respectively) and Labedella endophytica CPCC 203961T (98.9% and 99.0%, respectively).
31402073	6	64	dep	19176T	670:675	arg1	%					692:692	98.9%	688:692	98.9%	688:692	The 16S rRNA gene sequences of the strains showed highest similarities with Labedella gwakjiensis KCTC 19176T (99.2% and 98.9%, respectively) and Labedella endophytica CPCC 203961T (98.9% and 99.0%, respectively).
31402073	9	65	from	species	1305:1311	arg1	strains					1262:1268	the strains	1258:1268	the strains from each other and from the other species of the genus Labedella with values below the thresholds for species delineation	1258:1391	Genomic analyses, including average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH), clearly separated the strains from each other and from the other species of the genus Labedella with values below the thresholds for species delineation.
31402073	6	66	theme	highest	617:623	arg1	similarities					625:636	highest similarities	617:636	highest similarities with Labedella gwakjiensis KCTC 19176T (99.2% and 98.9%, respectively) and Labedella endophytica CPCC 203961T (98.9% and 99.0%, respectively)	617:778	The 16S rRNA gene sequences of the strains showed highest similarities with Labedella gwakjiensis KCTC 19176T (99.2% and 98.9%, respectively) and Labedella endophytica CPCC 203961T (98.9% and 99.0%, respectively).
31402073	10	67	with	agreement	1477:1485	arg1	description					1496:1506	the description	1492:1506	the description of the genus Labedella	1492:1529	The two strains showed chemotaxonomic characteristics and phenotypic properties in agreement with the description of the genus Labedella and also confirmed the differentiation from the closest species.
31402073	8	68	dep	L.	1107:1108	arg1	endophytica					1110:1120	endophytica	1110:1120	endophytica	1110:1120	Phylogenetic analyses based on 16S rRNA gene sequences and single-copy phylogenetic marker genes (pMGs) showed that the two strains belonged to the genus Labedella and formed a separate cluster from the closest species L. gwakjiensis KCTC 19176T and L. endophytica CPCC 203961T.
31402073	11	69	theme	Labedella	1730:1738	arg1	sp					1751:1752	Labedella phragmitis sp	1730:1752	Labedella phragmitis sp	1730:1752	The data demonstrated that strains 11W25H-1T and 8H24J-4-2T represented two novel species of the genus Labedella, for which the names Labedella phragmitis sp.
31402073	12	70	dep	nov	1755:1757	arg1	populi					1824:1829	populi	1824:1829	populi	1824:1829	nov. (type strain 11W25H-1T=JCM 33144T=CGMCC 1.16700T) and Labedella populi sp.
31402073	0	71	theme	phragmitis	10:19	arg1	sp					21:22	Labedella phragmitis sp	0:22	Labedella phragmitis sp.	0:23	Labedella phragmitis sp.
31402073	6	72	with	similarities	625:636	arg1	203961T					740:746	Labedella endophytica CPCC 203961T	713:746	Labedella endophytica CPCC 203961T (98.9% and 99.0%, respectively)	713:778	The 16S rRNA gene sequences of the strains showed highest similarities with Labedella gwakjiensis KCTC 19176T (99.2% and 98.9%, respectively) and Labedella endophytica CPCC 203961T (98.9% and 99.0%, respectively).
31402073	6	72	with	similarities	625:636	arg1	19176T					670:675	Labedella gwakjiensis KCTC 19176T	643:675	Labedella gwakjiensis KCTC 19176T (99.2% and 98.9%, respectively)	643:707	The 16S rRNA gene sequences of the strains showed highest similarities with Labedella gwakjiensis KCTC 19176T (99.2% and 98.9%, respectively) and Labedella endophytica CPCC 203961T (98.9% and 99.0%, respectively).
31402073	8	73	theme	separate	1034:1041	arg1	cluster					1043:1049	a separate cluster	1032:1049	a separate cluster from the closest species L. gwakjiensis KCTC 19176T and L. endophytica CPCC 203961T	1032:1133	Phylogenetic analyses based on 16S rRNA gene sequences and single-copy phylogenetic marker genes (pMGs) showed that the two strains belonged to the genus Labedella and formed a separate cluster from the closest species L. gwakjiensis KCTC 19176T and L. endophytica CPCC 203961T.
31402073	8	74	theme	Phylogenetic	857:868	arg1	analyses					870:877	Phylogenetic analyses	857:877	Phylogenetic analyses based on 16S rRNA gene sequences and single-copy phylogenetic marker genes (pMGs)	857:959	Phylogenetic analyses based on 16S rRNA gene sequences and single-copy phylogenetic marker genes (pMGs) showed that the two strains belonged to the genus Labedella and formed a separate cluster from the closest species L. gwakjiensis KCTC 19176T and L. endophytica CPCC 203961T.
31402073	11	75	theme	novel	1672:1676	arg1	species					1678:1684	two novel species	1668:1684	two novel species	1668:1684	The data demonstrated that strains 11W25H-1T and 8H24J-4-2T represented two novel species of the genus Labedella, for which the names Labedella phragmitis sp.
31402073	10	76	theme	chemotaxonomic	1417:1430	arg1	characteristics					1432:1446	chemotaxonomic characteristics	1417:1446	chemotaxonomic characteristics	1417:1446	The two strains showed chemotaxonomic characteristics and phenotypic properties in agreement with the description of the genus Labedella and also confirmed the differentiation from the closest species.
31402073	7	77	dep	strains	813:819	arg1	11W25H-1T					821:829	11W25H-1T	821:829	11W25H-1T	821:829	The sequence similarity between strains 11W25H-1T and 8H24J-4-2T was 99.4%.
31402073	7	77	dep	strains	813:819	arg1	strains					813:819	strains 11W25H-1T and 8H24J-4-2T	813:844	strains 11W25H-1T and 8H24J-4-2T	813:844	The sequence similarity between strains 11W25H-1T and 8H24J-4-2T was 99.4%.
31402073	7	77	dep	strains	813:819	arg1	8H24J-4-2T					835:844	8H24J-4-2T	835:844	8H24J-4-2T	835:844	The sequence similarity between strains 11W25H-1T and 8H24J-4-2T was 99.4%.
31402073	9	78	theme	other	1299:1303	arg1	species					1305:1311	the other species	1295:1311	the other species of the genus Labedella	1295:1334	Genomic analyses, including average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH), clearly separated the strains from each other and from the other species of the genus Labedella with values below the thresholds for species delineation.
31402073	8	79	theme	closest	1060:1066	arg1	19176T					1096:1101	L. gwakjiensis KCTC 19176T	1076:1101	L. gwakjiensis KCTC 19176T	1076:1101	Phylogenetic analyses based on 16S rRNA gene sequences and single-copy phylogenetic marker genes (pMGs) showed that the two strains belonged to the genus Labedella and formed a separate cluster from the closest species L. gwakjiensis KCTC 19176T and L. endophytica CPCC 203961T.
31402073	8	79	theme	closest	1060:1066	arg1	species					1068:1074	the closest species L. gwakjiensis KCTC 19176T and L. endophytica CPCC 203961T	1056:1133	the closest species L. gwakjiensis KCTC 19176T and L. endophytica CPCC 203961T	1056:1133	Phylogenetic analyses based on 16S rRNA gene sequences and single-copy phylogenetic marker genes (pMGs) showed that the two strains belonged to the genus Labedella and formed a separate cluster from the closest species L. gwakjiensis KCTC 19176T and L. endophytica CPCC 203961T.
31402073	8	79	theme	closest	1060:1066	arg1	203961T					1127:1133	L. endophytica CPCC 203961T	1107:1133	L. endophytica CPCC 203961T	1107:1133	Phylogenetic analyses based on 16S rRNA gene sequences and single-copy phylogenetic marker genes (pMGs) showed that the two strains belonged to the genus Labedella and formed a separate cluster from the closest species L. gwakjiensis KCTC 19176T and L. endophytica CPCC 203961T.
31402073	12	80	theme	type	1761:1764	arg1	1.16700T					1800:1807	type strain 11W25H-1T=JCM 33144T=CGMCC 1.16700T	1761:1807	type strain 11W25H-1T=JCM 33144T=CGMCC 1.16700T	1761:1807	nov. (type strain 11W25H-1T=JCM 33144T=CGMCC 1.16700T) and Labedella populi sp.
31402073	12	80	theme	type	1761:1764	arg1	nov					1755:1757	nov	1755:1757	nov	1755:1757	nov. (type strain 11W25H-1T=JCM 33144T=CGMCC 1.16700T) and Labedella populi sp.
31402073	11	81	theme	genus	1693:1697	arg1	Labedella					1699:1707	the genus Labedella	1689:1707	the genus Labedella	1689:1707	The data demonstrated that strains 11W25H-1T and 8H24J-4-2T represented two novel species of the genus Labedella, for which the names Labedella phragmitis sp.
31402073	8	82	theme	L.	1076:1077	arg1	19176T					1096:1101	L. gwakjiensis KCTC 19176T	1076:1101	L. gwakjiensis KCTC 19176T	1076:1101	Phylogenetic analyses based on 16S rRNA gene sequences and single-copy phylogenetic marker genes (pMGs) showed that the two strains belonged to the genus Labedella and formed a separate cluster from the closest species L. gwakjiensis KCTC 19176T and L. endophytica CPCC 203961T.
31402073	8	82	theme	L.	1076:1077	arg1	species					1068:1074	the closest species L. gwakjiensis KCTC 19176T and L. endophytica CPCC 203961T	1056:1133	the closest species L. gwakjiensis KCTC 19176T and L. endophytica CPCC 203961T	1056:1133	Phylogenetic analyses based on 16S rRNA gene sequences and single-copy phylogenetic marker genes (pMGs) showed that the two strains belonged to the genus Labedella and formed a separate cluster from the closest species L. gwakjiensis KCTC 19176T and L. endophytica CPCC 203961T.
31402073	1	83	theme	nov.	25:28	arg1	populi					44:49	nov. and Labedella populi	25:49	nov. and Labedella populi	25:49	nov. and Labedella populi sp.
31402073	8	84	theme	gene	897:900	arg1	sequences					902:910	16S rRNA gene sequences	888:910	16S rRNA gene sequences	888:910	Phylogenetic analyses based on 16S rRNA gene sequences and single-copy phylogenetic marker genes (pMGs) showed that the two strains belonged to the genus Labedella and formed a separate cluster from the closest species L. gwakjiensis KCTC 19176T and L. endophytica CPCC 203961T.
31402073	6	85	theme	endophytica	723:733	arg1	203961T					740:746	Labedella endophytica CPCC 203961T	713:746	Labedella endophytica CPCC 203961T (98.9% and 99.0%, respectively)	713:778	The 16S rRNA gene sequences of the strains showed highest similarities with Labedella gwakjiensis KCTC 19176T (99.2% and 98.9%, respectively) and Labedella endophytica CPCC 203961T (98.9% and 99.0%, respectively).
31402073	9	86	theme	genus	1320:1324	arg1	Labedella					1326:1334	the genus Labedella	1316:1334	the genus Labedella	1316:1334	Genomic analyses, including average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH), clearly separated the strains from each other and from the other species of the genus Labedella with values below the thresholds for species delineation.
31402073	8	87	theme	KCTC	1091:1094	arg1	19176T					1096:1101	L. gwakjiensis KCTC 19176T	1076:1101	L. gwakjiensis KCTC 19176T	1076:1101	Phylogenetic analyses based on 16S rRNA gene sequences and single-copy phylogenetic marker genes (pMGs) showed that the two strains belonged to the genus Labedella and formed a separate cluster from the closest species L. gwakjiensis KCTC 19176T and L. endophytica CPCC 203961T.
31402073	8	87	theme	KCTC	1091:1094	arg1	species					1068:1074	the closest species L. gwakjiensis KCTC 19176T and L. endophytica CPCC 203961T	1056:1133	the closest species L. gwakjiensis KCTC 19176T and L. endophytica CPCC 203961T	1056:1133	Phylogenetic analyses based on 16S rRNA gene sequences and single-copy phylogenetic marker genes (pMGs) showed that the two strains belonged to the genus Labedella and formed a separate cluster from the closest species L. gwakjiensis KCTC 19176T and L. endophytica CPCC 203961T.
31402073	1	88	theme	Labedella	34:42	arg1	populi					44:49	nov. and Labedella populi	25:49	nov. and Labedella populi	25:49	nov. and Labedella populi sp.
31402073	6	89	theme	16S	571:573	arg1	sequences					585:593	The 16S rRNA gene sequences	567:593	The 16S rRNA gene sequences of the strains	567:608	The 16S rRNA gene sequences of the strains showed highest similarities with Labedella gwakjiensis KCTC 19176T (99.2% and 98.9%, respectively) and Labedella endophytica CPCC 203961T (98.9% and 99.0%, respectively).
30201231	15	0	theme	=	2496:2496	arg1	IC50					2491:2494	IC50	2491:2494	IC50 = 28.01 μg/mL	2491:2508	It also evidenced anti-inflammatory potential in RAW 264.7 macrophages, presenting capacity for nitric oxide reduction (IC50 = 28.01 μg/mL).
30201231	15	0	theme	=	2496:2496	arg1	reduction					2480:2488	nitric oxide reduction	2467:2488	nitric oxide reduction (IC50 = 28.01 μg/mL)	2467:2509	It also evidenced anti-inflammatory potential in RAW 264.7 macrophages, presenting capacity for nitric oxide reduction (IC50 = 28.01 μg/mL).
30201231	22	1	theme	good	3398:3401	arg1	Ethanol					3376:3382	Ethanol	3376:3382	Ethanol	3376:3382	Ethanol seems to be a good option for the extraction of these bioactive compounds, since relevant antioxidant/anti-radical and anti-inflammatory activities were found for this extract.
30201231	22	1	theme	good	3398:3401	arg1	option					3403:3408	a good option	3396:3408	a good option for the extraction of these bioactive compounds	3396:3456	Ethanol seems to be a good option for the extraction of these bioactive compounds, since relevant antioxidant/anti-radical and anti-inflammatory activities were found for this extract.
30201231	6	2	theme	constituents	1324:1335	arg1	quantification					1302:1315	The quantification	1298:1315	The quantification of the constituents	1298:1335	The quantification of the constituents was performed using two external standards (2,4-dihydroxy-1,4-benzoxazin-3-one and verbascoside).
30201231	13	3	theme	compounds	2217:2225	arg1	%					2202:2202	about 4.39%	2192:2202	about 4.39% of the total compounds identified	2192:2236	Significant concentrations were also found for phenylpropanoids, which constitute about 4.39% of the total compounds identified.
30201231	13	3	theme	compounds	2217:2225	arg1	compounds					2217:2225	the total compounds	2207:2225	the total compounds identified	2207:2236	Significant concentrations were also found for phenylpropanoids, which constitute about 4.39% of the total compounds identified.
30201231	12	4	theme	main	2080:2083	arg1	compound					2085:2092	the main compound	2076:2092	the main compound of the extract	2076:2107	DIBOA was the main compound of the extract.
30201231	12	4	theme	main	2080:2083	arg1	DIBOA					2066:2070	DIBOA	2066:2070	DIBOA	2066:2070	DIBOA was the main compound of the extract.
30201231	4	5	theme	High	902:905	arg1	Chromatography					926:939	High Performance Liquid Chromatography	902:939	High Performance Liquid Chromatography with Photodiode Array Detector coupled to Electrospray Ionization Mass Spectrometry (HPLC-PDA-ESI/MSn)	902:1042	MATERIAL AND METHODS In this study, an 96% ethanol extract from Acanthus mollis leaves was obtained and its phytochemical composition evaluated using High Performance Liquid Chromatography with Photodiode Array Detector coupled to Electrospray Ionization Mass Spectrometry (HPLC-PDA-ESI/MSn).
30201231	21	6	theme	anti-inflammatory	3312:3328	arg1	activity					3330:3337	a significant anti-inflammatory activity	3298:3337	a significant anti-inflammatory activity that was never previously described	3298:3373	Among the compounds identified in this study, benzoxazinoids exhibited a significant anti-inflammatory activity that was never previously described.
30201231	22	7	theme	compounds	3448:3456	arg1	extraction					3418:3427	the extraction	3414:3427	the extraction of these bioactive compounds	3414:3456	Ethanol seems to be a good option for the extraction of these bioactive compounds, since relevant antioxidant/anti-radical and anti-inflammatory activities were found for this extract.
30201231	3	8	theme	proposed	559:566	arg1	work					568:571	The proposed work	555:571	The proposed work	555:571	AIM OF THE STUDY The proposed work aims to perform a phytochemical characterization and evaluation of the therapeutic potential of Acanthus mollis, based on biological properties that support its traditional uses.
30201231	5	9	theme	heteronuclear	1250:1262	arg1	correlation					1278:1288	heteronuclear multiple-bond correlation	1250:1288	heteronuclear multiple-bond correlation (HMBC)	1250:1295	The chemical structure of the compound isolated was elucidated using 1H and 13C Nuclear Magnetic Resonance (NMR), 1H-correlation spectroscopy (1H-COSY), heteronuclear single quantum correlation (HSQC) and heteronuclear multiple-bond correlation (HMBC).
30201231	5	9	theme	heteronuclear	1250:1262	arg1	HMBC					1291:1294	HMBC	1291:1294	HMBC	1291:1294	The chemical structure of the compound isolated was elucidated using 1H and 13C Nuclear Magnetic Resonance (NMR), 1H-correlation spectroscopy (1H-COSY), heteronuclear single quantum correlation (HSQC) and heteronuclear multiple-bond correlation (HMBC).
30201231	17	10	theme	inflammatory	2801:2812	arg1	diseases					2822:2829	skin inflammatory related diseases	2796:2829	skin inflammatory related diseases	2796:2829	Importantly, all effective concentrations were devoid of cytotoxicity in keratinocytes, thus highlighting the safety of the extract for the treatment of skin inflammatory related diseases.
30201231	7	11	theme	DPPH	1513:1516	arg1	assay					1519:1523	the 2,2-diphenyl-1-pycrylhydrazyl (DPPH) assay	1478:1523	the 2,2-diphenyl-1-pycrylhydrazyl (DPPH) assay	1478:1523	The antioxidant activity was determined by the 2,2-diphenyl-1-pycrylhydrazyl (DPPH) assay.
30201231	3	12	theme	STUDY	549:553	arg1	AIM					538:540	AIM	538:540	AIM OF THE STUDY The proposed work aims to perform a phytochemical characterization and evaluation of the therapeutic potential of Acanthus mollis, based on biological properties that support its traditional uses.	538:750	AIM OF THE STUDY The proposed work aims to perform a phytochemical characterization and evaluation of the therapeutic potential of Acanthus mollis, based on biological properties that support its traditional uses.
30201231	4	13	theme	phytochemical	860:872	arg1	composition					874:884	its phytochemical composition	856:884	its phytochemical composition	856:884	MATERIAL AND METHODS In this study, an 96% ethanol extract from Acanthus mollis leaves was obtained and its phytochemical composition evaluated using High Performance Liquid Chromatography with Photodiode Array Detector coupled to Electrospray Ionization Mass Spectrometry (HPLC-PDA-ESI/MSn).
30201231	1	14	theme	urinary	288:294	arg1	tract					296:300	the digestive and urinary tract	270:300	tract	296:300	ETHNOPHARMACOLOGICAL RELEVANCE Acanthus mollis is a plant native to the Mediterranean region, traditionally used as diuretic, anti-inflammatory and soothing of the mucous membranes of the digestive and urinary tract and externally as healing of wounds and burns, also demonstrating analgesic and anti-inflammatory activities.
30201231	7	15	theme	2,2-diphenyl-1-pycrylhydrazyl	1482:1510	arg1	assay					1519:1523	the 2,2-diphenyl-1-pycrylhydrazyl (DPPH) assay	1478:1523	the 2,2-diphenyl-1-pycrylhydrazyl (DPPH) assay	1478:1523	The antioxidant activity was determined by the 2,2-diphenyl-1-pycrylhydrazyl (DPPH) assay.
30201231	19	16	theme	anti-inflammatory	3033:3049	arg1	activity					3051:3058	a considerable anti-inflammatory activity	3018:3058	a considerable anti-inflammatory activity	3018:3058	The benzoxazinoid DIBOA demonstrated a considerable anti-inflammatory activity suggesting its important contribution to this activity.
30201231	19	17	theme	benzoxazinoid	2985:2997	arg1	DIBOA					2999:3003	The benzoxazinoid DIBOA	2981:3003	The benzoxazinoid DIBOA	2981:3003	The benzoxazinoid DIBOA demonstrated a considerable anti-inflammatory activity suggesting its important contribution to this activity.
30201231	4	18	theme	Array	957:961	arg1	Detector					963:970	Photodiode Array Detector	946:970	Photodiode Array Detector coupled to Electrospray Ionization Mass Spectrometry (HPLC-PDA-ESI/MSn)	946:1042	MATERIAL AND METHODS In this study, an 96% ethanol extract from Acanthus mollis leaves was obtained and its phytochemical composition evaluated using High Performance Liquid Chromatography with Photodiode Array Detector coupled to Electrospray Ionization Mass Spectrometry (HPLC-PDA-ESI/MSn).
30201231	22	19	theme	bioactive	3438:3446	arg1	compounds					3448:3456	these bioactive compounds	3432:3456	these bioactive compounds	3432:3456	Ethanol seems to be a good option for the extraction of these bioactive compounds, since relevant antioxidant/anti-radical and anti-inflammatory activities were found for this extract.
30201231	8	20	theme	Anti-inflammatory	1526:1542	arg1	activity					1544:1551	Anti-inflammatory activity	1526:1551	Anti-inflammatory activity	1526:1551	Anti-inflammatory activity was determined measuring the inhibition of nitric oxide production by RAW 264.7 macrophages stimulated with the TLR4 agonist lipopolysaccharide (LPS) and through lipoxygenase (LOX) inhibition assay.
30201231	19	21	theme	important	3075:3083	arg1	contribution					3085:3096	its important contribution	3071:3096	its important contribution to this activity	3071:3113	The benzoxazinoid DIBOA demonstrated a considerable anti-inflammatory activity suggesting its important contribution to this activity.
30201231	8	22	theme	LOX	1729:1731	arg1	assay					1745:1749	lipoxygenase (LOX) inhibition assay	1715:1749	lipoxygenase (LOX) inhibition assay	1715:1749	Anti-inflammatory activity was determined measuring the inhibition of nitric oxide production by RAW 264.7 macrophages stimulated with the TLR4 agonist lipopolysaccharide (LPS) and through lipoxygenase (LOX) inhibition assay.
30201231	5	23	theme	Nuclear	1125:1131	arg1	NMR					1153:1155	NMR	1153:1155	NMR	1153:1155	The chemical structure of the compound isolated was elucidated using 1H and 13C Nuclear Magnetic Resonance (NMR), 1H-correlation spectroscopy (1H-COSY), heteronuclear single quantum correlation (HSQC) and heteronuclear multiple-bond correlation (HMBC).
30201231	5	23	theme	Nuclear	1125:1131	arg1	Resonance					1142:1150	Nuclear Magnetic Resonance	1125:1150	Nuclear Magnetic Resonance (NMR)	1125:1156	The chemical structure of the compound isolated was elucidated using 1H and 13C Nuclear Magnetic Resonance (NMR), 1H-correlation spectroscopy (1H-COSY), heteronuclear single quantum correlation (HSQC) and heteronuclear multiple-bond correlation (HMBC).
30201231	21	24	theme	significant	3300:3310	arg1	activity					3330:3337	a significant anti-inflammatory activity	3298:3337	a significant anti-inflammatory activity that was never previously described	3298:3373	Among the compounds identified in this study, benzoxazinoids exhibited a significant anti-inflammatory activity that was never previously described.
30201231	16	25	dep	in	2522:2523	arg1	vitro					2525:2529	vitro	2525:2529	vitro	2525:2529	Moreover, in vitro studies have shown that this extract was able to inhibit the lipoxygenase, with an IC50 of 104.39 ± 4.95 µg/mL.
30201231	15	26	dep	=	2496:2496	arg1	28.01 μg/mL					2498:2508	28.01 μg/mL	2498:2508	28.01 μg/mL	2498:2508	It also evidenced anti-inflammatory potential in RAW 264.7 macrophages, presenting capacity for nitric oxide reduction (IC50 = 28.01 μg/mL).
30201231	4	27	theme	Liquid	919:924	arg1	Chromatography					926:939	High Performance Liquid Chromatography	902:939	High Performance Liquid Chromatography with Photodiode Array Detector coupled to Electrospray Ionization Mass Spectrometry (HPLC-PDA-ESI/MSn)	902:1042	MATERIAL AND METHODS In this study, an 96% ethanol extract from Acanthus mollis leaves was obtained and its phytochemical composition evaluated using High Performance Liquid Chromatography with Photodiode Array Detector coupled to Electrospray Ionization Mass Spectrometry (HPLC-PDA-ESI/MSn).
30201231	1	28	theme	tract	296:300	arg1	membranes					257:265	the mucous membranes	246:265	the mucous membranes of the digestive and urinary tract	246:300	ETHNOPHARMACOLOGICAL RELEVANCE Acanthus mollis is a plant native to the Mediterranean region, traditionally used as diuretic, anti-inflammatory and soothing of the mucous membranes of the digestive and urinary tract and externally as healing of wounds and burns, also demonstrating analgesic and anti-inflammatory activities.
30201231	7	29	theme	antioxidant	1439:1449	arg1	activity					1451:1458	The antioxidant activity	1435:1458	The antioxidant activity	1435:1458	The antioxidant activity was determined by the 2,2-diphenyl-1-pycrylhydrazyl (DPPH) assay.
30201231	3	30	theme	phytochemical	591:603	arg1	characterization					605:620	a phytochemical characterization	589:620	a phytochemical characterization	589:620	AIM OF THE STUDY The proposed work aims to perform a phytochemical characterization and evaluation of the therapeutic potential of Acanthus mollis, based on biological properties that support its traditional uses.
30201231	4	31	from	mollis	825:830	arg1	extract					803:809	an 96% ethanol extract	788:809	an 96% ethanol extract from Acanthus mollis leaves was obtained	788:850	MATERIAL AND METHODS In this study, an 96% ethanol extract from Acanthus mollis leaves was obtained and its phytochemical composition evaluated using High Performance Liquid Chromatography with Photodiode Array Detector coupled to Electrospray Ionization Mass Spectrometry (HPLC-PDA-ESI/MSn).
30201231	4	31	from	mollis	825:830	arg1	METHODS					765:771	METHODS	765:771	METHODS	765:771	MATERIAL AND METHODS In this study, an 96% ethanol extract from Acanthus mollis leaves was obtained and its phytochemical composition evaluated using High Performance Liquid Chromatography with Photodiode Array Detector coupled to Electrospray Ionization Mass Spectrometry (HPLC-PDA-ESI/MSn).
30201231	4	31	from	mollis	825:830	arg1	composition					874:884	its phytochemical composition	856:884	its phytochemical composition	856:884	MATERIAL AND METHODS In this study, an 96% ethanol extract from Acanthus mollis leaves was obtained and its phytochemical composition evaluated using High Performance Liquid Chromatography with Photodiode Array Detector coupled to Electrospray Ionization Mass Spectrometry (HPLC-PDA-ESI/MSn).
30201231	4	31	from	mollis	825:830	arg1	MATERIAL					752:759	MATERIAL	752:759	MATERIAL	752:759	MATERIAL AND METHODS In this study, an 96% ethanol extract from Acanthus mollis leaves was obtained and its phytochemical composition evaluated using High Performance Liquid Chromatography with Photodiode Array Detector coupled to Electrospray Ionization Mass Spectrometry (HPLC-PDA-ESI/MSn).
30201231	18	32	dep	possible	2867:2874	arg1	30 µg/mL					2970:2977	30 µg/mL	2970:2977	30 µg/mL	2970:2977	Concerning macrophages it was also possible to disclose concentrations showing anti-inflammatory activity and without cytotoxicity (up to 30 µg/mL).
30201231	17	33	theme	extract	2767:2773	arg1	safety					2753:2758	the safety	2749:2758	the safety of the extract for the treatment of skin inflammatory related diseases	2749:2829	Importantly, all effective concentrations were devoid of cytotoxicity in keratinocytes, thus highlighting the safety of the extract for the treatment of skin inflammatory related diseases.
30201231	3	34	theme	therapeutic	644:654	arg1	potential					656:664	the therapeutic potential	640:664	the therapeutic potential of Acanthus mollis	640:683	AIM OF THE STUDY The proposed work aims to perform a phytochemical characterization and evaluation of the therapeutic potential of Acanthus mollis, based on biological properties that support its traditional uses.
30201231	9	35	theme	resazurin	1827:1835	arg1	assay					1837:1841	the resazurin assay	1823:1841	the resazurin assay	1823:1841	The cytotoxicity was screened on two lines (RAW 264.7 and HaCaT) using the resazurin assay.
30201231	1	36	theme	Mediterranean	158:170	arg1	region					172:177	the Mediterranean region	154:177	the Mediterranean region	154:177	ETHNOPHARMACOLOGICAL RELEVANCE Acanthus mollis is a plant native to the Mediterranean region, traditionally used as diuretic, anti-inflammatory and soothing of the mucous membranes of the digestive and urinary tract and externally as healing of wounds and burns, also demonstrating analgesic and anti-inflammatory activities.
30201231	16	37	theme	104.39 ± 4.95 µg/mL	2622:2640	arg1	IC50					2614:2617	an IC50	2611:2617	an IC50 of 104.39 ± 4.95 µg/mL	2611:2640	Moreover, in vitro studies have shown that this extract was able to inhibit the lipoxygenase, with an IC50 of 104.39 ± 4.95 µg/mL.
30201231	2	38	theme	mollis	510:515	arg1	efficacy					517:524	Acanthus mollis efficacy	501:524	Acanthus mollis efficacy	501:524	However, studies focused on its phytochemical composition as well as scientific proof of Acanthus mollis efficacy are scarce.
30201231	8	39	theme	nitric	1596:1601	arg1	oxide					1603:1607	nitric oxide	1596:1607	nitric oxide production	1596:1618	Anti-inflammatory activity was determined measuring the inhibition of nitric oxide production by RAW 264.7 macrophages stimulated with the TLR4 agonist lipopolysaccharide (LPS) and through lipoxygenase (LOX) inhibition assay.
30201231	4	40	theme	Electrospray	983:994	arg1	HPLC-PDA-ESI/MSn					1026:1041	HPLC-PDA-ESI/MSn	1026:1041	HPLC-PDA-ESI/MSn	1026:1041	MATERIAL AND METHODS In this study, an 96% ethanol extract from Acanthus mollis leaves was obtained and its phytochemical composition evaluated using High Performance Liquid Chromatography with Photodiode Array Detector coupled to Electrospray Ionization Mass Spectrometry (HPLC-PDA-ESI/MSn).
30201231	4	40	theme	Electrospray	983:994	arg1	Spectrometry					1012:1023	Electrospray Ionization Mass Spectrometry	983:1023	Electrospray Ionization Mass Spectrometry (HPLC-PDA-ESI/MSn)	983:1042	MATERIAL AND METHODS In this study, an 96% ethanol extract from Acanthus mollis leaves was obtained and its phytochemical composition evaluated using High Performance Liquid Chromatography with Photodiode Array Detector coupled to Electrospray Ionization Mass Spectrometry (HPLC-PDA-ESI/MSn).
30201231	8	41	theme	production	1609:1618	arg1	inhibition					1582:1591	the inhibition	1578:1591	the inhibition of nitric oxide production by RAW 264.7 macrophages stimulated with the TLR4 agonist lipopolysaccharide (LPS) and through lipoxygenase (LOX) inhibition assay	1578:1749	Anti-inflammatory activity was determined measuring the inhibition of nitric oxide production by RAW 264.7 macrophages stimulated with the TLR4 agonist lipopolysaccharide (LPS) and through lipoxygenase (LOX) inhibition assay.
30201231	22	42	theme	relevant	3465:3472	arg1	activities					3521:3530	relevant antioxidant/anti-radical and anti-inflammatory activities	3465:3530	relevant antioxidant/anti-radical and anti-inflammatory activities	3465:3530	Ethanol seems to be a good option for the extraction of these bioactive compounds, since relevant antioxidant/anti-radical and anti-inflammatory activities were found for this extract.
30201231	4	43	theme	Mass	1007:1010	arg1	HPLC-PDA-ESI/MSn					1026:1041	HPLC-PDA-ESI/MSn	1026:1041	HPLC-PDA-ESI/MSn	1026:1041	MATERIAL AND METHODS In this study, an 96% ethanol extract from Acanthus mollis leaves was obtained and its phytochemical composition evaluated using High Performance Liquid Chromatography with Photodiode Array Detector coupled to Electrospray Ionization Mass Spectrometry (HPLC-PDA-ESI/MSn).
30201231	4	43	theme	Mass	1007:1010	arg1	Spectrometry					1012:1023	Electrospray Ionization Mass Spectrometry	983:1023	Electrospray Ionization Mass Spectrometry (HPLC-PDA-ESI/MSn)	983:1042	MATERIAL AND METHODS In this study, an 96% ethanol extract from Acanthus mollis leaves was obtained and its phytochemical composition evaluated using High Performance Liquid Chromatography with Photodiode Array Detector coupled to Electrospray Ionization Mass Spectrometry (HPLC-PDA-ESI/MSn).
30201231	8	44	theme	RAW	1623:1625	arg1	macrophages					1633:1643	RAW 264.7 macrophages	1623:1643	RAW 264.7 macrophages stimulated with the TLR4 agonist lipopolysaccharide (LPS)	1623:1701	Anti-inflammatory activity was determined measuring the inhibition of nitric oxide production by RAW 264.7 macrophages stimulated with the TLR4 agonist lipopolysaccharide (LPS) and through lipoxygenase (LOX) inhibition assay.
30201231	4	45	theme	96	791:792	arg1	%					793:793	%	793:793	%	793:793	MATERIAL AND METHODS In this study, an 96% ethanol extract from Acanthus mollis leaves was obtained and its phytochemical composition evaluated using High Performance Liquid Chromatography with Photodiode Array Detector coupled to Electrospray Ionization Mass Spectrometry (HPLC-PDA-ESI/MSn).
30201231	1	46	theme	ETHNOPHARMACOLOGICAL	86:105	arg1	mollis					126:131	ETHNOPHARMACOLOGICAL RELEVANCE Acanthus mollis	86:131	ETHNOPHARMACOLOGICAL RELEVANCE Acanthus mollis	86:131	ETHNOPHARMACOLOGICAL RELEVANCE Acanthus mollis is a plant native to the Mediterranean region, traditionally used as diuretic, anti-inflammatory and soothing of the mucous membranes of the digestive and urinary tract and externally as healing of wounds and burns, also demonstrating analgesic and anti-inflammatory activities.
30201231	1	46	theme	ETHNOPHARMACOLOGICAL	86:105	arg1	plant					138:142	a plant	136:142	a plant native to the Mediterranean region, traditionally used as diuretic, anti-inflammatory and soothing of the mucous membranes of the digestive and urinary tract and externally as healing of wounds and burns, also demonstrating analgesic and anti-inflammatory activities	136:409	ETHNOPHARMACOLOGICAL RELEVANCE Acanthus mollis is a plant native to the Mediterranean region, traditionally used as diuretic, anti-inflammatory and soothing of the mucous membranes of the digestive and urinary tract and externally as healing of wounds and burns, also demonstrating analgesic and anti-inflammatory activities.
30201231	5	47	theme	single	1212:1217	arg1	HSQC					1240:1243	HSQC	1240:1243	HSQC	1240:1243	The chemical structure of the compound isolated was elucidated using 1H and 13C Nuclear Magnetic Resonance (NMR), 1H-correlation spectroscopy (1H-COSY), heteronuclear single quantum correlation (HSQC) and heteronuclear multiple-bond correlation (HMBC).
30201231	5	47	theme	single	1212:1217	arg1	correlation					1227:1237	heteronuclear single quantum correlation	1198:1237	heteronuclear single quantum correlation (HSQC)	1198:1244	The chemical structure of the compound isolated was elucidated using 1H and 13C Nuclear Magnetic Resonance (NMR), 1H-correlation spectroscopy (1H-COSY), heteronuclear single quantum correlation (HSQC) and heteronuclear multiple-bond correlation (HMBC).
30201231	4	48	from	METHODS	765:771	arg1	study					781:785	this study	776:785	this study	776:785	MATERIAL AND METHODS In this study, an 96% ethanol extract from Acanthus mollis leaves was obtained and its phytochemical composition evaluated using High Performance Liquid Chromatography with Photodiode Array Detector coupled to Electrospray Ionization Mass Spectrometry (HPLC-PDA-ESI/MSn).
30201231	1	49	theme	Acanthus	117:124	arg1	mollis					126:131	ETHNOPHARMACOLOGICAL RELEVANCE Acanthus mollis	86:131	ETHNOPHARMACOLOGICAL RELEVANCE Acanthus mollis	86:131	ETHNOPHARMACOLOGICAL RELEVANCE Acanthus mollis is a plant native to the Mediterranean region, traditionally used as diuretic, anti-inflammatory and soothing of the mucous membranes of the digestive and urinary tract and externally as healing of wounds and burns, also demonstrating analgesic and anti-inflammatory activities.
30201231	1	49	theme	Acanthus	117:124	arg1	plant					138:142	a plant	136:142	a plant native to the Mediterranean region, traditionally used as diuretic, anti-inflammatory and soothing of the mucous membranes of the digestive and urinary tract and externally as healing of wounds and burns, also demonstrating analgesic and anti-inflammatory activities	136:409	ETHNOPHARMACOLOGICAL RELEVANCE Acanthus mollis is a plant native to the Mediterranean region, traditionally used as diuretic, anti-inflammatory and soothing of the mucous membranes of the digestive and urinary tract and externally as healing of wounds and burns, also demonstrating analgesic and anti-inflammatory activities.
30201231	17	50	theme	effective	2660:2668	arg1	concentrations					2670:2683	all effective concentrations	2656:2683	all effective concentrations	2656:2683	Importantly, all effective concentrations were devoid of cytotoxicity in keratinocytes, thus highlighting the safety of the extract for the treatment of skin inflammatory related diseases.
30201231	14	51	theme	IC50	2343:2346	arg1	anion					2336:2340	superoxide anion	2325:2340	superoxide anion (IC50 = 29.42 ± 1.99 μg/mL)	2325:2368	This extract showed antioxidant capacity against DPPH (IC50 = 40.00 ± 1.59 μg/mL) and superoxide anion (IC50 = 29.42 ± 1.99 μg/mL).
30201231	14	51	theme	IC50	2343:2346	arg1	29.42 ± 1.99 μg/mL					2350:2367	IC50 = 29.42 ± 1.99 μg/mL	2343:2367	IC50 = 29.42 ± 1.99 μg/mL	2343:2367	This extract showed antioxidant capacity against DPPH (IC50 = 40.00 ± 1.59 μg/mL) and superoxide anion (IC50 = 29.42 ± 1.99 μg/mL).
30201231	0	52	theme	Acanthus	15:22	arg1	mollis					24:29	Acanthus mollis	15:29	Acanthus mollis	15:29	Bioactivity of Acanthus mollis - Contribution of benzoxazinoids and phenylpropanoids.
30201231	8	53	theme	TLR4	1665:1668	arg1	LPS					1698:1700	LPS	1698:1700	LPS	1698:1700	Anti-inflammatory activity was determined measuring the inhibition of nitric oxide production by RAW 264.7 macrophages stimulated with the TLR4 agonist lipopolysaccharide (LPS) and through lipoxygenase (LOX) inhibition assay.
30201231	8	53	theme	TLR4	1665:1668	arg1	lipopolysaccharide					1678:1695	the TLR4 agonist lipopolysaccharide	1661:1695	the TLR4 agonist lipopolysaccharide (LPS)	1661:1701	Anti-inflammatory activity was determined measuring the inhibition of nitric oxide production by RAW 264.7 macrophages stimulated with the TLR4 agonist lipopolysaccharide (LPS) and through lipoxygenase (LOX) inhibition assay.
30201231	3	54	theme	mollis	678:683	arg1	potential					656:664	the therapeutic potential	640:664	the therapeutic potential of Acanthus mollis	640:683	AIM OF THE STUDY The proposed work aims to perform a phytochemical characterization and evaluation of the therapeutic potential of Acanthus mollis, based on biological properties that support its traditional uses.
30201231	9	55	dep	lines	1789:1793	arg1	RAW					1796:1798	RAW 264.7	1796:1804	RAW 264.7	1796:1804	The cytotoxicity was screened on two lines (RAW 264.7 and HaCaT) using the resazurin assay.
30201231	9	55	dep	lines	1789:1793	arg1	HaCaT					1810:1814	HaCaT	1810:1814	HaCaT	1810:1814	The cytotoxicity was screened on two lines (RAW 264.7 and HaCaT) using the resazurin assay.
30201231	9	55	dep	lines	1789:1793	arg1	lines					1789:1793	two lines	1785:1793	two lines (RAW 264.7 and HaCaT)	1785:1815	The cytotoxicity was screened on two lines (RAW 264.7 and HaCaT) using the resazurin assay.
30201231	3	56	dep	traditional	734:744	arg1	uses					746:749	uses	746:749	uses	746:749	AIM OF THE STUDY The proposed work aims to perform a phytochemical characterization and evaluation of the therapeutic potential of Acanthus mollis, based on biological properties that support its traditional uses.
30201231	2	57	theme	scientific	481:490	arg1	proof					492:496	scientific proof	481:496	studies focused on its phytochemical composition as well as scientific proof of Acanthus mollis efficacy	421:524	However, studies focused on its phytochemical composition as well as scientific proof of Acanthus mollis efficacy are scarce.
30201231	2	57	theme	scientific	481:490	arg1	studies					421:427	studies	421:427	studies focused on its phytochemical composition as well as scientific proof of Acanthus mollis efficacy	421:524	However, studies focused on its phytochemical composition as well as scientific proof of Acanthus mollis efficacy are scarce.
30201231	2	57	theme	scientific	481:490	arg1	scarce					530:535	scarce	530:535	scarce	530:535	However, studies focused on its phytochemical composition as well as scientific proof of Acanthus mollis efficacy are scarce.
30201231	18	58	theme	anti-inflammatory	2911:2927	arg1	activity					2929:2936	anti-inflammatory activity	2911:2936	anti-inflammatory activity	2911:2936	Concerning macrophages it was also possible to disclose concentrations showing anti-inflammatory activity and without cytotoxicity (up to 30 µg/mL).
30201231	3	59	theme	biological	695:704	arg1	properties					706:715	biological properties	695:715	biological properties that support its traditional uses	695:749	AIM OF THE STUDY The proposed work aims to perform a phytochemical characterization and evaluation of the therapeutic potential of Acanthus mollis, based on biological properties that support its traditional uses.
30201231	4	60	theme	ethanol	795:801	arg1	extract					803:809	an 96% ethanol extract	788:809	an 96% ethanol extract from Acanthus mollis leaves was obtained	788:850	MATERIAL AND METHODS In this study, an 96% ethanol extract from Acanthus mollis leaves was obtained and its phytochemical composition evaluated using High Performance Liquid Chromatography with Photodiode Array Detector coupled to Electrospray Ionization Mass Spectrometry (HPLC-PDA-ESI/MSn).
30201231	13	61	theme	Significant	2110:2120	arg1	concentrations					2122:2135	Significant concentrations	2110:2135	Significant concentrations	2110:2135	Significant concentrations were also found for phenylpropanoids, which constitute about 4.39% of the total compounds identified.
30201231	5	62	theme	multiple-bond	1264:1276	arg1	correlation					1278:1288	heteronuclear multiple-bond correlation	1250:1288	heteronuclear multiple-bond correlation (HMBC)	1250:1295	The chemical structure of the compound isolated was elucidated using 1H and 13C Nuclear Magnetic Resonance (NMR), 1H-correlation spectroscopy (1H-COSY), heteronuclear single quantum correlation (HSQC) and heteronuclear multiple-bond correlation (HMBC).
30201231	5	62	theme	multiple-bond	1264:1276	arg1	HMBC					1291:1294	HMBC	1291:1294	HMBC	1291:1294	The chemical structure of the compound isolated was elucidated using 1H and 13C Nuclear Magnetic Resonance (NMR), 1H-correlation spectroscopy (1H-COSY), heteronuclear single quantum correlation (HSQC) and heteronuclear multiple-bond correlation (HMBC).
30201231	14	63	theme	superoxide	2325:2334	arg1	anion					2336:2340	superoxide anion	2325:2340	superoxide anion (IC50 = 29.42 ± 1.99 μg/mL)	2325:2368	This extract showed antioxidant capacity against DPPH (IC50 = 40.00 ± 1.59 μg/mL) and superoxide anion (IC50 = 29.42 ± 1.99 μg/mL).
30201231	14	63	theme	superoxide	2325:2334	arg1	29.42 ± 1.99 μg/mL					2350:2367	IC50 = 29.42 ± 1.99 μg/mL	2343:2367	IC50 = 29.42 ± 1.99 μg/mL	2343:2367	This extract showed antioxidant capacity against DPPH (IC50 = 40.00 ± 1.59 μg/mL) and superoxide anion (IC50 = 29.42 ± 1.99 μg/mL).
30201231	10	64	theme	main	1948:1951	arg1	derivatives					1891:1901	its derivatives	1887:1901	its derivatives	1887:1901	RESULTS Compounds such as verbascoside and its derivatives, as well as benzoxazinoids were found as the main constituents.
30201231	10	64	theme	main	1948:1951	arg1	constituents					1953:1964	the main constituents	1944:1964	the main constituents	1944:1964	RESULTS Compounds such as verbascoside and its derivatives, as well as benzoxazinoids were found as the main constituents.
30201231	10	64	theme	main	1948:1951	arg1	Compounds					1852:1860	RESULTS Compounds	1844:1860	RESULTS Compounds such as verbascoside and its derivatives, as well as benzoxazinoids	1844:1928	RESULTS Compounds such as verbascoside and its derivatives, as well as benzoxazinoids were found as the main constituents.
30201231	10	64	theme	main	1948:1951	arg1	verbascoside					1870:1881	verbascoside	1870:1881	verbascoside	1870:1881	RESULTS Compounds such as verbascoside and its derivatives, as well as benzoxazinoids were found as the main constituents.
30201231	1	65	theme	mucous	250:255	arg1	membranes					257:265	the mucous membranes	246:265	the mucous membranes of the digestive and urinary tract	246:300	ETHNOPHARMACOLOGICAL RELEVANCE Acanthus mollis is a plant native to the Mediterranean region, traditionally used as diuretic, anti-inflammatory and soothing of the mucous membranes of the digestive and urinary tract and externally as healing of wounds and burns, also demonstrating analgesic and anti-inflammatory activities.
30201231	0	66	dep	Bioactivity	0:10	arg1	Contribution					33:44	Contribution	33:44	Bioactivity of Acanthus mollis - Contribution of benzoxazinoids and phenylpropanoids.	0:84	Bioactivity of Acanthus mollis - Contribution of benzoxazinoids and phenylpropanoids.
30201231	8	67	theme	lipoxygenase	1715:1726	arg1	assay					1745:1749	lipoxygenase (LOX) inhibition assay	1715:1749	lipoxygenase (LOX) inhibition assay	1715:1749	Anti-inflammatory activity was determined measuring the inhibition of nitric oxide production by RAW 264.7 macrophages stimulated with the TLR4 agonist lipopolysaccharide (LPS) and through lipoxygenase (LOX) inhibition assay.
30201231	14	68	dep	=	2299:2299	arg1	40.00 ± 1.59 μg/mL					2301:2318	40.00 ± 1.59 μg/mL	2301:2318	40.00 ± 1.59 μg/mL	2301:2318	This extract showed antioxidant capacity against DPPH (IC50 = 40.00 ± 1.59 μg/mL) and superoxide anion (IC50 = 29.42 ± 1.99 μg/mL).
30201231	15	69	theme	anti-inflammatory	2389:2405	arg1	potential					2407:2415	anti-inflammatory potential	2389:2415	anti-inflammatory potential in RAW 264.7 macrophages	2389:2440	It also evidenced anti-inflammatory potential in RAW 264.7 macrophages, presenting capacity for nitric oxide reduction (IC50 = 28.01 μg/mL).
30201231	15	70	theme	nitric	2467:2472	arg1	oxide					2474:2478	nitric oxide	2467:2478	nitric oxide reduction (IC50 = 28.01 μg/mL)	2467:2509	It also evidenced anti-inflammatory potential in RAW 264.7 macrophages, presenting capacity for nitric oxide reduction (IC50 = 28.01 μg/mL).
30201231	17	71	theme	diseases	2822:2829	arg1	treatment					2783:2791	the treatment	2779:2791	the treatment of skin inflammatory related diseases	2779:2829	Importantly, all effective concentrations were devoid of cytotoxicity in keratinocytes, thus highlighting the safety of the extract for the treatment of skin inflammatory related diseases.
30201231	20	72	theme	anti-inflammatory	3158:3174	arg1	properties					3176:3185	the anti-inflammatory properties	3154:3185	the anti-inflammatory properties traditionally attributed to this plant	3154:3224	CONCLUSIONS These results corroborate the anti-inflammatory properties traditionally attributed to this plant.
30201231	5	73	dep	1H	1114:1115	arg1	NMR					1153:1155	NMR	1153:1155	NMR	1153:1155	The chemical structure of the compound isolated was elucidated using 1H and 13C Nuclear Magnetic Resonance (NMR), 1H-correlation spectroscopy (1H-COSY), heteronuclear single quantum correlation (HSQC) and heteronuclear multiple-bond correlation (HMBC).
30201231	5	73	dep	1H	1114:1115	arg1	Resonance					1142:1150	Nuclear Magnetic Resonance	1125:1150	Nuclear Magnetic Resonance (NMR)	1125:1156	The chemical structure of the compound isolated was elucidated using 1H and 13C Nuclear Magnetic Resonance (NMR), 1H-correlation spectroscopy (1H-COSY), heteronuclear single quantum correlation (HSQC) and heteronuclear multiple-bond correlation (HMBC).
30201231	14	74	theme	=	2299:2299	arg1	IC50					2294:2297	IC50	2294:2297	IC50 = 40.00 ± 1.59 μg/mL	2294:2318	This extract showed antioxidant capacity against DPPH (IC50 = 40.00 ± 1.59 μg/mL) and superoxide anion (IC50 = 29.42 ± 1.99 μg/mL).
30201231	14	74	theme	=	2299:2299	arg1	DPPH					2288:2291	DPPH	2288:2291	DPPH (IC50 = 40.00 ± 1.59 μg/mL)	2288:2319	This extract showed antioxidant capacity against DPPH (IC50 = 40.00 ± 1.59 μg/mL) and superoxide anion (IC50 = 29.42 ± 1.99 μg/mL).
30201231	10	75	theme	RESULTS	1844:1850	arg1	derivatives					1891:1901	its derivatives	1887:1901	its derivatives	1887:1901	RESULTS Compounds such as verbascoside and its derivatives, as well as benzoxazinoids were found as the main constituents.
30201231	10	75	theme	RESULTS	1844:1850	arg1	constituents					1953:1964	the main constituents	1944:1964	the main constituents	1944:1964	RESULTS Compounds such as verbascoside and its derivatives, as well as benzoxazinoids were found as the main constituents.
30201231	10	75	theme	RESULTS	1844:1850	arg1	Compounds					1852:1860	RESULTS Compounds	1844:1860	RESULTS Compounds such as verbascoside and its derivatives, as well as benzoxazinoids	1844:1928	RESULTS Compounds such as verbascoside and its derivatives, as well as benzoxazinoids were found as the main constituents.
30201231	10	75	theme	RESULTS	1844:1850	arg1	verbascoside					1870:1881	verbascoside	1870:1881	verbascoside	1870:1881	RESULTS Compounds such as verbascoside and its derivatives, as well as benzoxazinoids were found as the main constituents.
30201231	1	76	theme	digestive	274:282	arg1	tract					296:300	the digestive and urinary tract	270:300	tract	296:300	ETHNOPHARMACOLOGICAL RELEVANCE Acanthus mollis is a plant native to the Mediterranean region, traditionally used as diuretic, anti-inflammatory and soothing of the mucous membranes of the digestive and urinary tract and externally as healing of wounds and burns, also demonstrating analgesic and anti-inflammatory activities.
30201231	17	77	theme	skin	2796:2799	arg1	diseases					2822:2829	skin inflammatory related diseases	2796:2829	skin inflammatory related diseases	2796:2829	Importantly, all effective concentrations were devoid of cytotoxicity in keratinocytes, thus highlighting the safety of the extract for the treatment of skin inflammatory related diseases.
30201231	8	78	theme	inhibition	1734:1743	arg1	assay					1745:1749	lipoxygenase (LOX) inhibition assay	1715:1749	lipoxygenase (LOX) inhibition assay	1715:1749	Anti-inflammatory activity was determined measuring the inhibition of nitric oxide production by RAW 264.7 macrophages stimulated with the TLR4 agonist lipopolysaccharide (LPS) and through lipoxygenase (LOX) inhibition assay.
30201231	17	79	theme	related	2814:2820	arg1	diseases					2822:2829	skin inflammatory related diseases	2796:2829	skin inflammatory related diseases	2796:2829	Importantly, all effective concentrations were devoid of cytotoxicity in keratinocytes, thus highlighting the safety of the extract for the treatment of skin inflammatory related diseases.
30201231	19	80	theme	considerable	3020:3031	arg1	activity					3051:3058	a considerable anti-inflammatory activity	3018:3058	a considerable anti-inflammatory activity	3018:3058	The benzoxazinoid DIBOA demonstrated a considerable anti-inflammatory activity suggesting its important contribution to this activity.
30201231	22	81	theme	anti-inflammatory	3503:3519	arg1	activities					3521:3530	relevant antioxidant/anti-radical and anti-inflammatory activities	3465:3530	relevant antioxidant/anti-radical and anti-inflammatory activities	3465:3530	Ethanol seems to be a good option for the extraction of these bioactive compounds, since relevant antioxidant/anti-radical and anti-inflammatory activities were found for this extract.
30201231	14	82	theme	antioxidant	2259:2269	arg1	capacity					2271:2278	antioxidant capacity	2259:2278	antioxidant capacity	2259:2278	This extract showed antioxidant capacity against DPPH (IC50 = 40.00 ± 1.59 μg/mL) and superoxide anion (IC50 = 29.42 ± 1.99 μg/mL).
30201231	4	83	theme	Photodiode	946:955	arg1	Array					957:961	Photodiode Array	946:961	Photodiode Array Detector coupled to Electrospray Ionization Mass Spectrometry (HPLC-PDA-ESI/MSn)	946:1042	MATERIAL AND METHODS In this study, an 96% ethanol extract from Acanthus mollis leaves was obtained and its phytochemical composition evaluated using High Performance Liquid Chromatography with Photodiode Array Detector coupled to Electrospray Ionization Mass Spectrometry (HPLC-PDA-ESI/MSn).
30201231	11	84	theme	DIBOA	2046:2050	arg1	derivatives					2053:2063	the 2,4-dihydroxy-1,4-benzoxazin-3-one (DIBOA) derivatives	2006:2063	the 2,4-dihydroxy-1,4-benzoxazin-3-one (DIBOA) derivatives	2006:2063	A percentage of 5.58% was verified for the 2,4-dihydroxy-1,4-benzoxazin-3-one (DIBOA) derivatives.
30201231	4	85	dep	mollis	825:830	arg1	leaves					832:837	leaves	832:837	leaves was obtained	832:850	MATERIAL AND METHODS In this study, an 96% ethanol extract from Acanthus mollis leaves was obtained and its phytochemical composition evaluated using High Performance Liquid Chromatography with Photodiode Array Detector coupled to Electrospray Ionization Mass Spectrometry (HPLC-PDA-ESI/MSn).
30201231	6	86	dep	standards	1370:1378	arg1	verbascoside					1420:1431	verbascoside	1420:1431	verbascoside	1420:1431	The quantification of the constituents was performed using two external standards (2,4-dihydroxy-1,4-benzoxazin-3-one and verbascoside).
30201231	6	86	dep	standards	1370:1378	arg1	2,4-dihydroxy-1,4-benzoxazin-3-one					1381:1414	2,4-dihydroxy-1,4-benzoxazin-3-one	1381:1414	2,4-dihydroxy-1,4-benzoxazin-3-one	1381:1414	The quantification of the constituents was performed using two external standards (2,4-dihydroxy-1,4-benzoxazin-3-one and verbascoside).
30201231	6	86	dep	standards	1370:1378	arg1	standards					1370:1378	two external standards	1357:1378	two external standards (2,4-dihydroxy-1,4-benzoxazin-3-one and verbascoside)	1357:1432	The quantification of the constituents was performed using two external standards (2,4-dihydroxy-1,4-benzoxazin-3-one and verbascoside).
30201231	15	87	from	potential	2407:2415	arg1	macrophages					2430:2440	RAW 264.7 macrophages	2420:2440	RAW 264.7 macrophages	2420:2440	It also evidenced anti-inflammatory potential in RAW 264.7 macrophages, presenting capacity for nitric oxide reduction (IC50 = 28.01 μg/mL).
30201231	1	88	theme	wounds	331:336	arg1	healing					320:326	healing	320:326	healing of wounds	320:336	ETHNOPHARMACOLOGICAL RELEVANCE Acanthus mollis is a plant native to the Mediterranean region, traditionally used as diuretic, anti-inflammatory and soothing of the mucous membranes of the digestive and urinary tract and externally as healing of wounds and burns, also demonstrating analgesic and anti-inflammatory activities.
30201231	1	88	theme	wounds	331:336	arg1	burns					342:346	burns	342:346	burns	342:346	ETHNOPHARMACOLOGICAL RELEVANCE Acanthus mollis is a plant native to the Mediterranean region, traditionally used as diuretic, anti-inflammatory and soothing of the mucous membranes of the digestive and urinary tract and externally as healing of wounds and burns, also demonstrating analgesic and anti-inflammatory activities.
30201231	6	89	theme	external	1361:1368	arg1	verbascoside					1420:1431	verbascoside	1420:1431	verbascoside	1420:1431	The quantification of the constituents was performed using two external standards (2,4-dihydroxy-1,4-benzoxazin-3-one and verbascoside).
30201231	6	89	theme	external	1361:1368	arg1	2,4-dihydroxy-1,4-benzoxazin-3-one					1381:1414	2,4-dihydroxy-1,4-benzoxazin-3-one	1381:1414	2,4-dihydroxy-1,4-benzoxazin-3-one	1381:1414	The quantification of the constituents was performed using two external standards (2,4-dihydroxy-1,4-benzoxazin-3-one and verbascoside).
30201231	6	89	theme	external	1361:1368	arg1	standards					1370:1378	two external standards	1357:1378	two external standards (2,4-dihydroxy-1,4-benzoxazin-3-one and verbascoside)	1357:1432	The quantification of the constituents was performed using two external standards (2,4-dihydroxy-1,4-benzoxazin-3-one and verbascoside).
30201231	5	90	theme	Magnetic	1133:1140	arg1	NMR					1153:1155	NMR	1153:1155	NMR	1153:1155	The chemical structure of the compound isolated was elucidated using 1H and 13C Nuclear Magnetic Resonance (NMR), 1H-correlation spectroscopy (1H-COSY), heteronuclear single quantum correlation (HSQC) and heteronuclear multiple-bond correlation (HMBC).
30201231	5	90	theme	Magnetic	1133:1140	arg1	Resonance					1142:1150	Nuclear Magnetic Resonance	1125:1150	Nuclear Magnetic Resonance (NMR)	1125:1156	The chemical structure of the compound isolated was elucidated using 1H and 13C Nuclear Magnetic Resonance (NMR), 1H-correlation spectroscopy (1H-COSY), heteronuclear single quantum correlation (HSQC) and heteronuclear multiple-bond correlation (HMBC).
30201231	11	91	theme	2,4-dihydroxy-1,4-benzoxazin-3-one	2010:2043	arg1	derivatives					2053:2063	the 2,4-dihydroxy-1,4-benzoxazin-3-one (DIBOA) derivatives	2006:2063	the 2,4-dihydroxy-1,4-benzoxazin-3-one (DIBOA) derivatives	2006:2063	A percentage of 5.58% was verified for the 2,4-dihydroxy-1,4-benzoxazin-3-one (DIBOA) derivatives.
30201231	4	92	theme	Performance	907:917	arg1	Chromatography					926:939	High Performance Liquid Chromatography	902:939	High Performance Liquid Chromatography with Photodiode Array Detector coupled to Electrospray Ionization Mass Spectrometry (HPLC-PDA-ESI/MSn)	902:1042	MATERIAL AND METHODS In this study, an 96% ethanol extract from Acanthus mollis leaves was obtained and its phytochemical composition evaluated using High Performance Liquid Chromatography with Photodiode Array Detector coupled to Electrospray Ionization Mass Spectrometry (HPLC-PDA-ESI/MSn).
30201231	1	93	theme	native	144:149	arg1	mollis					126:131	ETHNOPHARMACOLOGICAL RELEVANCE Acanthus mollis	86:131	ETHNOPHARMACOLOGICAL RELEVANCE Acanthus mollis	86:131	ETHNOPHARMACOLOGICAL RELEVANCE Acanthus mollis is a plant native to the Mediterranean region, traditionally used as diuretic, anti-inflammatory and soothing of the mucous membranes of the digestive and urinary tract and externally as healing of wounds and burns, also demonstrating analgesic and anti-inflammatory activities.
30201231	1	93	theme	native	144:149	arg1	plant					138:142	a plant	136:142	a plant native to the Mediterranean region, traditionally used as diuretic, anti-inflammatory and soothing of the mucous membranes of the digestive and urinary tract and externally as healing of wounds and burns, also demonstrating analgesic and anti-inflammatory activities	136:409	ETHNOPHARMACOLOGICAL RELEVANCE Acanthus mollis is a plant native to the Mediterranean region, traditionally used as diuretic, anti-inflammatory and soothing of the mucous membranes of the digestive and urinary tract and externally as healing of wounds and burns, also demonstrating analgesic and anti-inflammatory activities.
30201231	17	94	theme	cytotoxicity	2700:2711	arg1	devoid					2690:2695	devoid	2690:2695	devoid	2690:2695	Importantly, all effective concentrations were devoid of cytotoxicity in keratinocytes, thus highlighting the safety of the extract for the treatment of skin inflammatory related diseases.
30201231	0	95	theme	phenylpropanoids	68:83	arg1	Contribution					33:44	Contribution	33:44	Bioactivity of Acanthus mollis - Contribution of benzoxazinoids and phenylpropanoids.	0:84	Bioactivity of Acanthus mollis - Contribution of benzoxazinoids and phenylpropanoids.
30201231	20	96	dep	CONCLUSIONS	3116:3126	arg1	corroborate					3142:3152	corroborate	3142:3152	corroborate the anti-inflammatory properties traditionally attributed to this plant	3142:3224	CONCLUSIONS These results corroborate the anti-inflammatory properties traditionally attributed to this plant.
30201231	4	97	from	extract	803:809	arg1	study					781:785	this study	776:785	this study	776:785	MATERIAL AND METHODS In this study, an 96% ethanol extract from Acanthus mollis leaves was obtained and its phytochemical composition evaluated using High Performance Liquid Chromatography with Photodiode Array Detector coupled to Electrospray Ionization Mass Spectrometry (HPLC-PDA-ESI/MSn).
30201231	2	98	theme	efficacy	517:524	arg1	proof					492:496	scientific proof	481:496	studies focused on its phytochemical composition as well as scientific proof of Acanthus mollis efficacy	421:524	However, studies focused on its phytochemical composition as well as scientific proof of Acanthus mollis efficacy are scarce.
30201231	2	98	theme	efficacy	517:524	arg1	scarce					530:535	scarce	530:535	scarce	530:535	However, studies focused on its phytochemical composition as well as scientific proof of Acanthus mollis efficacy are scarce.
30201231	2	98	theme	efficacy	517:524	arg1	studies					421:427	studies	421:427	studies focused on its phytochemical composition as well as scientific proof of Acanthus mollis efficacy	421:524	However, studies focused on its phytochemical composition as well as scientific proof of Acanthus mollis efficacy are scarce.
30201231	8	99	theme	oxide	1603:1607	arg1	production					1609:1618	nitric oxide production	1596:1618	nitric oxide production	1596:1618	Anti-inflammatory activity was determined measuring the inhibition of nitric oxide production by RAW 264.7 macrophages stimulated with the TLR4 agonist lipopolysaccharide (LPS) and through lipoxygenase (LOX) inhibition assay.
30201231	5	100	theme	1H-correlation	1159:1172	arg1	1H-COSY					1188:1194	1H-COSY	1188:1194	1H-COSY	1188:1194	The chemical structure of the compound isolated was elucidated using 1H and 13C Nuclear Magnetic Resonance (NMR), 1H-correlation spectroscopy (1H-COSY), heteronuclear single quantum correlation (HSQC) and heteronuclear multiple-bond correlation (HMBC).
30201231	5	100	theme	1H-correlation	1159:1172	arg1	spectroscopy					1174:1185	1H-correlation spectroscopy	1159:1185	1H-correlation spectroscopy (1H-COSY)	1159:1195	The chemical structure of the compound isolated was elucidated using 1H and 13C Nuclear Magnetic Resonance (NMR), 1H-correlation spectroscopy (1H-COSY), heteronuclear single quantum correlation (HSQC) and heteronuclear multiple-bond correlation (HMBC).
30201231	2	101	theme	Acanthus	501:508	arg1	efficacy					517:524	Acanthus mollis efficacy	501:524	Acanthus mollis efficacy	501:524	However, studies focused on its phytochemical composition as well as scientific proof of Acanthus mollis efficacy are scarce.
30201231	4	102	from	composition	874:884	arg1	study					781:785	this study	776:785	this study	776:785	MATERIAL AND METHODS In this study, an 96% ethanol extract from Acanthus mollis leaves was obtained and its phytochemical composition evaluated using High Performance Liquid Chromatography with Photodiode Array Detector coupled to Electrospray Ionization Mass Spectrometry (HPLC-PDA-ESI/MSn).
30201231	22	103	theme	antioxidant/anti-radical	3474:3497	arg1	activities					3521:3530	relevant antioxidant/anti-radical and anti-inflammatory activities	3465:3530	relevant antioxidant/anti-radical and anti-inflammatory activities	3465:3530	Ethanol seems to be a good option for the extraction of these bioactive compounds, since relevant antioxidant/anti-radical and anti-inflammatory activities were found for this extract.
30201231	1	104	theme	analgesic	368:376	arg1	activities					400:409	analgesic and anti-inflammatory activities	368:409	analgesic and anti-inflammatory activities	368:409	ETHNOPHARMACOLOGICAL RELEVANCE Acanthus mollis is a plant native to the Mediterranean region, traditionally used as diuretic, anti-inflammatory and soothing of the mucous membranes of the digestive and urinary tract and externally as healing of wounds and burns, also demonstrating analgesic and anti-inflammatory activities.
30201231	15	105	theme	oxide	2474:2478	arg1	IC50					2491:2494	IC50	2491:2494	IC50 = 28.01 μg/mL	2491:2508	It also evidenced anti-inflammatory potential in RAW 264.7 macrophages, presenting capacity for nitric oxide reduction (IC50 = 28.01 μg/mL).
30201231	15	105	theme	oxide	2474:2478	arg1	reduction					2480:2488	nitric oxide reduction	2467:2488	nitric oxide reduction (IC50 = 28.01 μg/mL)	2467:2509	It also evidenced anti-inflammatory potential in RAW 264.7 macrophages, presenting capacity for nitric oxide reduction (IC50 = 28.01 μg/mL).
30201231	4	106	theme	Ionization	996:1005	arg1	HPLC-PDA-ESI/MSn					1026:1041	HPLC-PDA-ESI/MSn	1026:1041	HPLC-PDA-ESI/MSn	1026:1041	MATERIAL AND METHODS In this study, an 96% ethanol extract from Acanthus mollis leaves was obtained and its phytochemical composition evaluated using High Performance Liquid Chromatography with Photodiode Array Detector coupled to Electrospray Ionization Mass Spectrometry (HPLC-PDA-ESI/MSn).
30201231	4	106	theme	Ionization	996:1005	arg1	Spectrometry					1012:1023	Electrospray Ionization Mass Spectrometry	983:1023	Electrospray Ionization Mass Spectrometry (HPLC-PDA-ESI/MSn)	983:1042	MATERIAL AND METHODS In this study, an 96% ethanol extract from Acanthus mollis leaves was obtained and its phytochemical composition evaluated using High Performance Liquid Chromatography with Photodiode Array Detector coupled to Electrospray Ionization Mass Spectrometry (HPLC-PDA-ESI/MSn).
30201231	11	107	theme	%	1987:1987	arg1	percentage					1969:1978	A percentage	1967:1978	A percentage of 5.58%	1967:1987	A percentage of 5.58% was verified for the 2,4-dihydroxy-1,4-benzoxazin-3-one (DIBOA) derivatives.
30201231	1	108	theme	anti-inflammatory	382:398	arg1	activities					400:409	analgesic and anti-inflammatory activities	368:409	analgesic and anti-inflammatory activities	368:409	ETHNOPHARMACOLOGICAL RELEVANCE Acanthus mollis is a plant native to the Mediterranean region, traditionally used as diuretic, anti-inflammatory and soothing of the mucous membranes of the digestive and urinary tract and externally as healing of wounds and burns, also demonstrating analgesic and anti-inflammatory activities.
30201231	1	109	theme	RELEVANCE	107:115	arg1	mollis					126:131	ETHNOPHARMACOLOGICAL RELEVANCE Acanthus mollis	86:131	ETHNOPHARMACOLOGICAL RELEVANCE Acanthus mollis	86:131	ETHNOPHARMACOLOGICAL RELEVANCE Acanthus mollis is a plant native to the Mediterranean region, traditionally used as diuretic, anti-inflammatory and soothing of the mucous membranes of the digestive and urinary tract and externally as healing of wounds and burns, also demonstrating analgesic and anti-inflammatory activities.
30201231	1	109	theme	RELEVANCE	107:115	arg1	plant					138:142	a plant	136:142	a plant native to the Mediterranean region, traditionally used as diuretic, anti-inflammatory and soothing of the mucous membranes of the digestive and urinary tract and externally as healing of wounds and burns, also demonstrating analgesic and anti-inflammatory activities	136:409	ETHNOPHARMACOLOGICAL RELEVANCE Acanthus mollis is a plant native to the Mediterranean region, traditionally used as diuretic, anti-inflammatory and soothing of the mucous membranes of the digestive and urinary tract and externally as healing of wounds and burns, also demonstrating analgesic and anti-inflammatory activities.
30201231	17	110	from	cytotoxicity	2700:2711	arg1	keratinocytes					2716:2728	keratinocytes	2716:2728	keratinocytes	2716:2728	Importantly, all effective concentrations were devoid of cytotoxicity in keratinocytes, thus highlighting the safety of the extract for the treatment of skin inflammatory related diseases.
30201231	14	111	theme	=	2348:2348	arg1	anion					2336:2340	superoxide anion	2325:2340	superoxide anion (IC50 = 29.42 ± 1.99 μg/mL)	2325:2368	This extract showed antioxidant capacity against DPPH (IC50 = 40.00 ± 1.59 μg/mL) and superoxide anion (IC50 = 29.42 ± 1.99 μg/mL).
30201231	14	111	theme	=	2348:2348	arg1	29.42 ± 1.99 μg/mL					2350:2367	IC50 = 29.42 ± 1.99 μg/mL	2343:2367	IC50 = 29.42 ± 1.99 μg/mL	2343:2367	This extract showed antioxidant capacity against DPPH (IC50 = 40.00 ± 1.59 μg/mL) and superoxide anion (IC50 = 29.42 ± 1.99 μg/mL).
30201231	0	112	theme	mollis	24:29	arg1	Bioactivity					0:10	Bioactivity	0:10	Bioactivity of Acanthus mollis - Contribution of benzoxazinoids and phenylpropanoids.	0:84	Bioactivity of Acanthus mollis - Contribution of benzoxazinoids and phenylpropanoids.
30201231	5	113	theme	quantum	1219:1225	arg1	HSQC					1240:1243	HSQC	1240:1243	HSQC	1240:1243	The chemical structure of the compound isolated was elucidated using 1H and 13C Nuclear Magnetic Resonance (NMR), 1H-correlation spectroscopy (1H-COSY), heteronuclear single quantum correlation (HSQC) and heteronuclear multiple-bond correlation (HMBC).
30201231	5	113	theme	quantum	1219:1225	arg1	correlation					1227:1237	heteronuclear single quantum correlation	1198:1237	heteronuclear single quantum correlation (HSQC)	1198:1244	The chemical structure of the compound isolated was elucidated using 1H and 13C Nuclear Magnetic Resonance (NMR), 1H-correlation spectroscopy (1H-COSY), heteronuclear single quantum correlation (HSQC) and heteronuclear multiple-bond correlation (HMBC).
30201231	3	114	dep	AIM	538:540	arg1	aims					573:576	aims	573:576	aims to perform a phytochemical characterization and evaluation of the therapeutic potential of Acanthus mollis, based on biological properties that support its traditional uses	573:749	AIM OF THE STUDY The proposed work aims to perform a phytochemical characterization and evaluation of the therapeutic potential of Acanthus mollis, based on biological properties that support its traditional uses.
30201231	4	115	with	Chromatography	926:939	arg1	Detector					963:970	Photodiode Array Detector	946:970	Photodiode Array Detector coupled to Electrospray Ionization Mass Spectrometry (HPLC-PDA-ESI/MSn)	946:1042	MATERIAL AND METHODS In this study, an 96% ethanol extract from Acanthus mollis leaves was obtained and its phytochemical composition evaluated using High Performance Liquid Chromatography with Photodiode Array Detector coupled to Electrospray Ionization Mass Spectrometry (HPLC-PDA-ESI/MSn).
30201231	2	116	theme	phytochemical	444:456	arg1	composition					458:468	its phytochemical composition	440:468	its phytochemical composition	440:468	However, studies focused on its phytochemical composition as well as scientific proof of Acanthus mollis efficacy are scarce.
30201231	8	117	theme	agonist	1670:1676	arg1	LPS					1698:1700	LPS	1698:1700	LPS	1698:1700	Anti-inflammatory activity was determined measuring the inhibition of nitric oxide production by RAW 264.7 macrophages stimulated with the TLR4 agonist lipopolysaccharide (LPS) and through lipoxygenase (LOX) inhibition assay.
30201231	8	117	theme	agonist	1670:1676	arg1	lipopolysaccharide					1678:1695	the TLR4 agonist lipopolysaccharide	1661:1695	the TLR4 agonist lipopolysaccharide (LPS)	1661:1701	Anti-inflammatory activity was determined measuring the inhibition of nitric oxide production by RAW 264.7 macrophages stimulated with the TLR4 agonist lipopolysaccharide (LPS) and through lipoxygenase (LOX) inhibition assay.
30201231	3	118	theme	Acanthus	669:676	arg1	mollis					678:683	Acanthus mollis	669:683	Acanthus mollis	669:683	AIM OF THE STUDY The proposed work aims to perform a phytochemical characterization and evaluation of the therapeutic potential of Acanthus mollis, based on biological properties that support its traditional uses.
30201231	3	119	theme	potential	656:664	arg1	characterization					605:620	a phytochemical characterization	589:620	a phytochemical characterization	589:620	AIM OF THE STUDY The proposed work aims to perform a phytochemical characterization and evaluation of the therapeutic potential of Acanthus mollis, based on biological properties that support its traditional uses.
30201231	3	119	theme	potential	656:664	arg1	evaluation					626:635	evaluation	626:635	evaluation	626:635	AIM OF THE STUDY The proposed work aims to perform a phytochemical characterization and evaluation of the therapeutic potential of Acanthus mollis, based on biological properties that support its traditional uses.
30201231	4	120	theme	Acanthus	816:823	arg1	mollis					825:830	Acanthus mollis	816:830	Acanthus mollis leaves was obtained	816:850	MATERIAL AND METHODS In this study, an 96% ethanol extract from Acanthus mollis leaves was obtained and its phytochemical composition evaluated using High Performance Liquid Chromatography with Photodiode Array Detector coupled to Electrospray Ionization Mass Spectrometry (HPLC-PDA-ESI/MSn).
30201231	0	121	theme	benzoxazinoids	49:62	arg1	Contribution					33:44	Contribution	33:44	Bioactivity of Acanthus mollis - Contribution of benzoxazinoids and phenylpropanoids.	0:84	Bioactivity of Acanthus mollis - Contribution of benzoxazinoids and phenylpropanoids.
30201231	5	122	theme	compound	1075:1082	arg1	structure					1058:1066	The chemical structure	1045:1066	The chemical structure of the compound isolated	1045:1091	The chemical structure of the compound isolated was elucidated using 1H and 13C Nuclear Magnetic Resonance (NMR), 1H-correlation spectroscopy (1H-COSY), heteronuclear single quantum correlation (HSQC) and heteronuclear multiple-bond correlation (HMBC).
30201231	13	123	theme	total	2211:2215	arg1	compounds					2217:2225	the total compounds	2207:2225	the total compounds identified	2207:2236	Significant concentrations were also found for phenylpropanoids, which constitute about 4.39% of the total compounds identified.
30201231	4	124	from	MATERIAL	752:759	arg1	study					781:785	this study	776:785	this study	776:785	MATERIAL AND METHODS In this study, an 96% ethanol extract from Acanthus mollis leaves was obtained and its phytochemical composition evaluated using High Performance Liquid Chromatography with Photodiode Array Detector coupled to Electrospray Ionization Mass Spectrometry (HPLC-PDA-ESI/MSn).
30201231	5	125	theme	heteronuclear	1198:1210	arg1	HSQC					1240:1243	HSQC	1240:1243	HSQC	1240:1243	The chemical structure of the compound isolated was elucidated using 1H and 13C Nuclear Magnetic Resonance (NMR), 1H-correlation spectroscopy (1H-COSY), heteronuclear single quantum correlation (HSQC) and heteronuclear multiple-bond correlation (HMBC).
30201231	5	125	theme	heteronuclear	1198:1210	arg1	correlation					1227:1237	heteronuclear single quantum correlation	1198:1237	heteronuclear single quantum correlation (HSQC)	1198:1244	The chemical structure of the compound isolated was elucidated using 1H and 13C Nuclear Magnetic Resonance (NMR), 1H-correlation spectroscopy (1H-COSY), heteronuclear single quantum correlation (HSQC) and heteronuclear multiple-bond correlation (HMBC).
30201231	4	126	theme	%	793:793	arg1	extract					803:809	an 96% ethanol extract	788:809	an 96% ethanol extract from Acanthus mollis leaves was obtained	788:850	MATERIAL AND METHODS In this study, an 96% ethanol extract from Acanthus mollis leaves was obtained and its phytochemical composition evaluated using High Performance Liquid Chromatography with Photodiode Array Detector coupled to Electrospray Ionization Mass Spectrometry (HPLC-PDA-ESI/MSn).
30201231	16	127	with	able	2572:2575	arg1	IC50					2614:2617	an IC50	2611:2617	an IC50 of 104.39 ± 4.95 µg/mL	2611:2640	Moreover, in vitro studies have shown that this extract was able to inhibit the lipoxygenase, with an IC50 of 104.39 ± 4.95 µg/mL.
30201231	12	128	theme	extract	2101:2107	arg1	compound					2085:2092	the main compound	2076:2092	the main compound of the extract	2076:2107	DIBOA was the main compound of the extract.
30201231	12	128	theme	extract	2101:2107	arg1	DIBOA					2066:2070	DIBOA	2066:2070	DIBOA	2066:2070	DIBOA was the main compound of the extract.
30201231	5	129	theme	chemical	1049:1056	arg1	structure					1058:1066	The chemical structure	1045:1066	The chemical structure of the compound isolated	1045:1091	The chemical structure of the compound isolated was elucidated using 1H and 13C Nuclear Magnetic Resonance (NMR), 1H-correlation spectroscopy (1H-COSY), heteronuclear single quantum correlation (HSQC) and heteronuclear multiple-bond correlation (HMBC).
30201231	15	130	theme	RAW	2420:2422	arg1	macrophages					2430:2440	RAW 264.7 macrophages	2420:2440	RAW 264.7 macrophages	2420:2440	It also evidenced anti-inflammatory potential in RAW 264.7 macrophages, presenting capacity for nitric oxide reduction (IC50 = 28.01 μg/mL).
30201231	16	131	theme	in	2522:2523	arg1	studies					2531:2537	in vitro studies	2522:2537	in vitro studies	2522:2537	Moreover, in vitro studies have shown that this extract was able to inhibit the lipoxygenase, with an IC50 of 104.39 ± 4.95 µg/mL.
30201231	1	132	theme	membranes	257:265	arg1	soothing					234:241	soothing	234:241	soothing	234:241	ETHNOPHARMACOLOGICAL RELEVANCE Acanthus mollis is a plant native to the Mediterranean region, traditionally used as diuretic, anti-inflammatory and soothing of the mucous membranes of the digestive and urinary tract and externally as healing of wounds and burns, also demonstrating analgesic and anti-inflammatory activities.
30474914	0	0	theme	Liver	100:104	arg1	Injury					106:111	Acute Liver Injury	94:111	Acute Liver Injury	94:111	Proteomic Profiling of LPS-Induced Macrophage-Derived Exosomes Indicates Their Involvement in Acute Liver Injury.
30474914	4	1	theme	iTRAQ	529:533	arg1	spectrometry					541:552	relative and absolute quantification (iTRAQ) mass spectrometry	491:552	relative and absolute quantification (iTRAQ) mass spectrometry	491:552	The macrophage-exosomes are validated and the protein composition of those exosomes are investigated by isobaric tags for relative and absolute quantification (iTRAQ) mass spectrometry.
30474914	8	2	theme	metabolic	1034:1042	arg1	process					1044:1050	metabolic process	1034:1050	metabolic process	1034:1050	The bioinformatics analysis also indicates that these proteins are mainly involved in cellular process, single-organism process, metabolic process, and biological regulation.
30474914	6	3	theme	reduced	664:670	arg1	proteins					672:679	363 reduced proteins	660:679	363 reduced proteins in LPS-treated macrophage-exosomes	660:714	We discovered 341 increased proteins and 363 reduced proteins in LPS-treated macrophage-exosomes compared with control exosomes.
30474914	10	4	theme	NLRP3	1318:1322	arg1	inflammasome					1324:1335	NLRP3 inflammasome	1318:1335	NLRP3 inflammasome	1318:1335	Finally, hepatocytes can uptake macrophage-exosomes and subsequently NLRP3 inflammasome is activated in vitro and in vivo.
30474914	0	5	theme	Acute	94:98	arg1	Injury					106:111	Acute Liver Injury	94:111	Acute Liver Injury	94:111	Proteomic Profiling of LPS-Induced Macrophage-Derived Exosomes Indicates Their Involvement in Acute Liver Injury.
30474914	4	6	theme	mass	536:539	arg1	spectrometry					541:552	relative and absolute quantification (iTRAQ) mass spectrometry	491:552	relative and absolute quantification (iTRAQ) mass spectrometry	491:552	The macrophage-exosomes are validated and the protein composition of those exosomes are investigated by isobaric tags for relative and absolute quantification (iTRAQ) mass spectrometry.
30474914	4	7	theme	protein	415:421	arg1	composition					423:433	the protein composition	411:433	the protein composition of those exosomes	411:451	The macrophage-exosomes are validated and the protein composition of those exosomes are investigated by isobaric tags for relative and absolute quantification (iTRAQ) mass spectrometry.
30474914	12	8	theme	new	1522:1524	arg1	insights					1526:1533	new insights	1522:1533	new insights into macrophage-derived exosomes	1522:1566	Therefore, the iTRAQ proteomic strategy brings new insights into macrophage-derived exosomes.
30474914	7	9	theme	ontology	766:773	arg1	analysis					775:782	gene ontology analysis	761:782	gene ontology analysis	761:782	In addition, gene ontology analysis demonstrates that macrophage-exosomes proteins are mostly linked to cell, organelle, extracellular region, and membrane.
30474914	4	10	theme	absolute	504:511	arg1	spectrometry					541:552	relative and absolute quantification (iTRAQ) mass spectrometry	491:552	relative and absolute quantification (iTRAQ) mass spectrometry	491:552	The macrophage-exosomes are validated and the protein composition of those exosomes are investigated by isobaric tags for relative and absolute quantification (iTRAQ) mass spectrometry.
30474914	8	11	theme	cellular	991:998	arg1	process					1000:1006	cellular process	991:1006	cellular process	991:1006	The bioinformatics analysis also indicates that these proteins are mainly involved in cellular process, single-organism process, metabolic process, and biological regulation.
30474914	12	12	theme	macrophage-derived	1540:1557	arg1	exosomes					1559:1566	macrophage-derived exosomes	1540:1566	macrophage-derived exosomes	1540:1566	Therefore, the iTRAQ proteomic strategy brings new insights into macrophage-derived exosomes.
30474914	8	13	theme	biological	1057:1066	arg1	regulation					1068:1077	biological regulation	1057:1077	biological regulation	1057:1077	The bioinformatics analysis also indicates that these proteins are mainly involved in cellular process, single-organism process, metabolic process, and biological regulation.
30474914	6	14	theme	increased	637:645	arg1	proteins					647:654	341 increased proteins	633:654	341 increased proteins	633:654	We discovered 341 increased proteins and 363 reduced proteins in LPS-treated macrophage-exosomes compared with control exosomes.
30474914	11	15	theme	sepsis-induced	1446:1459	arg1	injury					1467:1472	sepsis-induced liver injury	1446:1472	sepsis-induced liver injury	1446:1472	These data emphasize the fundamental importance of macrophage-exosomes in sepsis-induced liver injury.
30474914	4	16	theme	quantification	513:526	arg1	spectrometry					541:552	relative and absolute quantification (iTRAQ) mass spectrometry	491:552	relative and absolute quantification (iTRAQ) mass spectrometry	491:552	The macrophage-exosomes are validated and the protein composition of those exosomes are investigated by isobaric tags for relative and absolute quantification (iTRAQ) mass spectrometry.
30474914	12	17	theme	iTRAQ	1490:1494	arg1	strategy					1506:1513	the iTRAQ proteomic strategy	1486:1513	the iTRAQ proteomic strategy	1486:1513	Therefore, the iTRAQ proteomic strategy brings new insights into macrophage-derived exosomes.
30474914	13	18	theme	possible	1646:1653	arg1	use					1655:1657	their possible use	1640:1657	their possible use as therapeutic targets for sepsis	1640:1691	It may improve our understanding of macrophage-exosomes' functions and their possible use as therapeutic targets for sepsis.
30474914	0	19	theme	Proteomic	0:8	arg1	Profiling					10:18	Proteomic Profiling	0:18	Proteomic Profiling of LPS-Induced Macrophage-Derived Exosomes	0:61	Proteomic Profiling of LPS-Induced Macrophage-Derived Exosomes Indicates Their Involvement in Acute Liver Injury.
30474914	7	20	theme	extracellular	869:881	arg1	region					883:888	extracellular region	869:888	extracellular region	869:888	In addition, gene ontology analysis demonstrates that macrophage-exosomes proteins are mostly linked to cell, organelle, extracellular region, and membrane.
30474914	11	21	theme	fundamental	1397:1407	arg1	importance					1409:1418	the fundamental importance	1393:1418	the fundamental importance of macrophage-exosomes in sepsis-induced liver injury	1393:1472	These data emphasize the fundamental importance of macrophage-exosomes in sepsis-induced liver injury.
30474914	11	22	theme	liver	1461:1465	arg1	injury					1467:1472	sepsis-induced liver injury	1446:1472	sepsis-induced liver injury	1446:1472	These data emphasize the fundamental importance of macrophage-exosomes in sepsis-induced liver injury.
30474914	7	23	theme	gene	761:764	arg1	ontology					766:773	gene ontology	761:773	gene ontology analysis	761:782	In addition, gene ontology analysis demonstrates that macrophage-exosomes proteins are mostly linked to cell, organelle, extracellular region, and membrane.
30474914	0	24	theme	LPS-Induced	23:33	arg1	Exosomes					54:61	LPS-Induced Macrophage-Derived Exosomes	23:61	LPS-Induced Macrophage-Derived Exosomes	23:61	Proteomic Profiling of LPS-Induced Macrophage-Derived Exosomes Indicates Their Involvement in Acute Liver Injury.
30474914	9	25	theme	NOD-like	1212:1219	arg1	pathway					1240:1246	the NOD-like receptor signaling pathway	1208:1246	the NOD-like receptor signaling pathway	1208:1246	Among these 341 upregulated proteins, Kyoto Encyclopedia of Genes and Genomes analysis reveals that 22 proteins are involved in the NOD-like receptor signaling pathway.
30474914	11	26	theme	macrophage-exosomes	1423:1441	arg1	importance					1409:1418	the fundamental importance	1393:1418	the fundamental importance of macrophage-exosomes in sepsis-induced liver injury	1393:1472	These data emphasize the fundamental importance of macrophage-exosomes in sepsis-induced liver injury.
30474914	11	27	from	importance	1409:1418	arg1	injury					1467:1472	sepsis-induced liver injury	1446:1472	sepsis-induced liver injury	1446:1472	These data emphasize the fundamental importance of macrophage-exosomes in sepsis-induced liver injury.
30474914	3	28	theme	exosomes	296:303	arg1	comparison					282:291	the molecular comparison	268:291	the molecular comparison of exosomes derived from LPS-induced macrophage	268:339	However, the molecular comparison of exosomes derived from LPS-induced macrophage has not been well analyzed.
30474914	2	29	theme	key	206:208	arg1	role					210:213	a key role	204:213	a key role	204:213	Macrophages play a key role in lipopolysaccharide (LPS)-induced sepsis.
30474914	12	30	theme	proteomic	1496:1504	arg1	strategy					1506:1513	the iTRAQ proteomic strategy	1486:1513	the iTRAQ proteomic strategy	1486:1513	Therefore, the iTRAQ proteomic strategy brings new insights into macrophage-derived exosomes.
30474914	6	31	theme	control	730:736	arg1	exosomes					738:745	control exosomes	730:745	control exosomes	730:745	We discovered 341 increased proteins and 363 reduced proteins in LPS-treated macrophage-exosomes compared with control exosomes.
30474914	0	32	theme	Exosomes	54:61	arg1	Profiling					10:18	Proteomic Profiling	0:18	Proteomic Profiling of LPS-Induced Macrophage-Derived Exosomes	0:61	Proteomic Profiling of LPS-Induced Macrophage-Derived Exosomes Indicates Their Involvement in Acute Liver Injury.
30474914	3	33	attach	derived	305:311	arg2	exosomes					296:303	exosomes	296:303	exosomes derived from LPS-induced macrophage	296:339	However, the molecular comparison of exosomes derived from LPS-induced macrophage has not been well analyzed.
30474914	3	33	attach	derived	305:311	arg1	macrophage					330:339	LPS-induced macrophage	318:339	LPS-induced macrophage	318:339	However, the molecular comparison of exosomes derived from LPS-induced macrophage has not been well analyzed.
30474914	3	34	theme	molecular	272:280	arg1	comparison					282:291	the molecular comparison	268:291	the molecular comparison of exosomes derived from LPS-induced macrophage	268:339	However, the molecular comparison of exosomes derived from LPS-induced macrophage has not been well analyzed.
30474914	4	35	theme	isobaric	473:480	arg1	tags					482:485	isobaric tags	473:485	isobaric tags for relative and absolute quantification (iTRAQ) mass spectrometry	473:552	The macrophage-exosomes are validated and the protein composition of those exosomes are investigated by isobaric tags for relative and absolute quantification (iTRAQ) mass spectrometry.
30474914	0	36	theme	Macrophage-Derived	35:52	arg1	Exosomes					54:61	LPS-Induced Macrophage-Derived Exosomes	23:61	LPS-Induced Macrophage-Derived Exosomes	23:61	Proteomic Profiling of LPS-Induced Macrophage-Derived Exosomes Indicates Their Involvement in Acute Liver Injury.
30474914	4	37	theme	relative	491:498	arg1	spectrometry					541:552	relative and absolute quantification (iTRAQ) mass spectrometry	491:552	relative and absolute quantification (iTRAQ) mass spectrometry	491:552	The macrophage-exosomes are validated and the protein composition of those exosomes are investigated by isobaric tags for relative and absolute quantification (iTRAQ) mass spectrometry.
30474914	2	38	theme	-induced	242:249	arg1	sepsis					251:256	lipopolysaccharide (LPS)-induced sepsis	218:256	lipopolysaccharide (LPS)-induced sepsis	218:256	Macrophages play a key role in lipopolysaccharide (LPS)-induced sepsis.
30474914	5	39	theme	5056	566:569	arg1	proteins					571:578	5056 proteins	566:578	5056 proteins	566:578	A total of 5056 proteins are identified in macrophage-exosomes.
30474914	12	40	link	macrophage-derived	1540:1557	arg1	exosomes					1559:1566	macrophage-derived exosomes	1540:1566	macrophage-derived exosomes	1540:1566	Therefore, the iTRAQ proteomic strategy brings new insights into macrophage-derived exosomes.
30474914	3	41	theme	LPS-induced	318:328	arg1	macrophage					330:339	LPS-induced macrophage	318:339	LPS-induced macrophage	318:339	However, the molecular comparison of exosomes derived from LPS-induced macrophage has not been well analyzed.
30474914	0	42	from	Involvement	79:89	arg1	Injury					106:111	Acute Liver Injury	94:111	Acute Liver Injury	94:111	Proteomic Profiling of LPS-Induced Macrophage-Derived Exosomes Indicates Their Involvement in Acute Liver Injury.
30474914	5	43	theme	proteins	571:578	arg1	total					557:561	A total	555:561	A total of 5056 proteins	555:578	A total of 5056 proteins are identified in macrophage-exosomes.
30474914	8	44	theme	single-organism	1009:1023	arg1	process					1025:1031	single-organism process	1009:1031	single-organism process	1009:1031	The bioinformatics analysis also indicates that these proteins are mainly involved in cellular process, single-organism process, metabolic process, and biological regulation.
30474914	6	45	from	proteins	647:654	arg1	macrophage-exosomes					696:714	LPS-treated macrophage-exosomes	684:714	LPS-treated macrophage-exosomes	684:714	We discovered 341 increased proteins and 363 reduced proteins in LPS-treated macrophage-exosomes compared with control exosomes.
30474914	9	46	theme	upregulated	1096:1106	arg1	proteins					1108:1115	these 341 upregulated proteins	1086:1115	these 341 upregulated proteins	1086:1115	Among these 341 upregulated proteins, Kyoto Encyclopedia of Genes and Genomes analysis reveals that 22 proteins are involved in the NOD-like receptor signaling pathway.
30474914	4	47	theme	exosomes	444:451	arg1	composition					423:433	the protein composition	411:433	the protein composition of those exosomes	411:451	The macrophage-exosomes are validated and the protein composition of those exosomes are investigated by isobaric tags for relative and absolute quantification (iTRAQ) mass spectrometry.
30474914	9	48	theme	receptor	1221:1228	arg1	pathway					1240:1246	the NOD-like receptor signaling pathway	1208:1246	the NOD-like receptor signaling pathway	1208:1246	Among these 341 upregulated proteins, Kyoto Encyclopedia of Genes and Genomes analysis reveals that 22 proteins are involved in the NOD-like receptor signaling pathway.
30474914	7	49	attach	linked	842:847	arg1	membrane					895:902	membrane	895:902	membrane	895:902	In addition, gene ontology analysis demonstrates that macrophage-exosomes proteins are mostly linked to cell, organelle, extracellular region, and membrane.
30474914	7	49	attach	linked	842:847	arg1	cell					852:855	cell	852:855	cell	852:855	In addition, gene ontology analysis demonstrates that macrophage-exosomes proteins are mostly linked to cell, organelle, extracellular region, and membrane.
30474914	7	49	attach	linked	842:847	arg2	proteins					822:829	macrophage-exosomes proteins	802:829	macrophage-exosomes proteins	802:829	In addition, gene ontology analysis demonstrates that macrophage-exosomes proteins are mostly linked to cell, organelle, extracellular region, and membrane.
30474914	7	49	attach	linked	842:847	arg1	organelle					858:866	organelle	858:866	organelle	858:866	In addition, gene ontology analysis demonstrates that macrophage-exosomes proteins are mostly linked to cell, organelle, extracellular region, and membrane.
30474914	7	49	attach	linked	842:847	arg1	region					883:888	extracellular region	869:888	extracellular region	869:888	In addition, gene ontology analysis demonstrates that macrophage-exosomes proteins are mostly linked to cell, organelle, extracellular region, and membrane.
30474914	10	50	dep	uptake	1274:1279	arg1	macrophage-exosomes					1281:1299	macrophage-exosomes	1281:1299	macrophage-exosomes	1281:1299	Finally, hepatocytes can uptake macrophage-exosomes and subsequently NLRP3 inflammasome is activated in vitro and in vivo.
30474914	13	51	theme	therapeutic	1662:1672	arg1	targets					1674:1680	therapeutic targets	1662:1680	therapeutic targets for sepsis	1662:1691	It may improve our understanding of macrophage-exosomes' functions and their possible use as therapeutic targets for sepsis.
30474914	9	52	theme	signaling	1230:1238	arg1	pathway					1240:1246	the NOD-like receptor signaling pathway	1208:1246	the NOD-like receptor signaling pathway	1208:1246	Among these 341 upregulated proteins, Kyoto Encyclopedia of Genes and Genomes analysis reveals that 22 proteins are involved in the NOD-like receptor signaling pathway.
30474914	1	53	theme	cellular	149:156	arg1	communication					158:170	cellular communication	149:170	cellular communication	149:170	Exosomes are typically involved in cellular communication and signaling.
30474914	6	54	theme	LPS-treated	684:694	arg1	macrophage-exosomes					696:714	LPS-treated macrophage-exosomes	684:714	LPS-treated macrophage-exosomes	684:714	We discovered 341 increased proteins and 363 reduced proteins in LPS-treated macrophage-exosomes compared with control exosomes.
30474914	6	55	from	proteins	672:679	arg1	macrophage-exosomes					696:714	LPS-treated macrophage-exosomes	684:714	LPS-treated macrophage-exosomes	684:714	We discovered 341 increased proteins and 363 reduced proteins in LPS-treated macrophage-exosomes compared with control exosomes.
30474914	9	56	theme	Encyclopedia	1124:1135	arg1	analysis					1158:1165	Kyoto Encyclopedia of Genes and Genomes analysis	1118:1165	Kyoto Encyclopedia of Genes and Genomes analysis	1118:1165	Among these 341 upregulated proteins, Kyoto Encyclopedia of Genes and Genomes analysis reveals that 22 proteins are involved in the NOD-like receptor signaling pathway.
30474914	7	57	theme	macrophage-exosomes	802:820	arg1	proteins					822:829	macrophage-exosomes proteins	802:829	macrophage-exosomes proteins	802:829	In addition, gene ontology analysis demonstrates that macrophage-exosomes proteins are mostly linked to cell, organelle, extracellular region, and membrane.
30474914	8	58	theme	bioinformatics	909:922	arg1	analysis					924:931	The bioinformatics analysis	905:931	The bioinformatics analysis	905:931	The bioinformatics analysis also indicates that these proteins are mainly involved in cellular process, single-organism process, metabolic process, and biological regulation.
29359978	0	0	theme	lactation	71:79	arg1	week					63:66	the first week	53:66	the first week of lactation on serum prolactin concentration, milk composition, and milk yield and on weight gain of their puppies	53:182	Effect of metoclopramide treatment of bitches during the first week of lactation on serum prolactin concentration, milk composition, and milk yield and on weight gain of their puppies.
29359978	11	1	theme	Puppy	1433:1437	arg1	gain					1446:1449	Puppy weight gain	1433:1449	Puppy weight gain	1433:1449	Puppy weight gain was not affected by metoclopramide treatment.
29359978	0	2	theme	weight	155:160	arg1	gain					162:165	weight gain	155:165	weight gain of their puppies	155:182	Effect of metoclopramide treatment of bitches during the first week of lactation on serum prolactin concentration, milk composition, and milk yield and on weight gain of their puppies.
29359978	3	3	theme	litter	626:631	arg1	puppy					613:617	the last puppy	604:617	the last puppy of the litter (day 0)	604:639	PROCEDURES 10 bitches received metoclopramide (0.2 mg/kg, PO, q 6 h for 6 days; treatment group) starting 10 to 24 hours after birth of the last puppy of the litter (day 0), and 10 bitches served as the control group.
29359978	1	4	from	effects	210:216	arg1	gain					384:387	puppy weight gain	371:387	puppy weight gain during early lactation	371:410	OBJECTIVE To investigate effects of metoclopramide orally administered to healthy bitches on serum prolactin and milk lactose concentrations, gross energy, and dry matter content and on puppy weight gain during early lactation.
29359978	1	4	from	effects	210:216	arg1	prolactin					284:292	serum prolactin and milk lactose concentrations	278:324	prolactin	284:292	OBJECTIVE To investigate effects of metoclopramide orally administered to healthy bitches on serum prolactin and milk lactose concentrations, gross energy, and dry matter content and on puppy weight gain during early lactation.
29359978	1	5	theme	metoclopramide	221:234	arg1	effects					210:216	effects	210:216	effects of metoclopramide orally administered to healthy bitches on serum prolactin and milk lactose concentrations, gross energy, and dry matter content and on puppy weight gain during early lactation	210:410	OBJECTIVE To investigate effects of metoclopramide orally administered to healthy bitches on serum prolactin and milk lactose concentrations, gross energy, and dry matter content and on puppy weight gain during early lactation.
29359978	2	6	theme	121	455:457	arg1	puppies					459:465	their 121 puppies	449:465	their 121 puppies	449:465	ANIMALS 20 client-owned bitches and their 121 puppies.
29359978	1	7	theme	gross	327:331	arg1	energy					333:338	gross energy	327:338	gross energy	327:338	OBJECTIVE To investigate effects of metoclopramide orally administered to healthy bitches on serum prolactin and milk lactose concentrations, gross energy, and dry matter content and on puppy weight gain during early lactation.
29359978	12	8	from	increase	1627:1634	arg1	concentration					1655:1667	serum prolactin concentration	1639:1667	serum prolactin concentration	1639:1667	CONCLUSIONS AND CLINICAL RELEVANCE Oral administration of metoclopramide to healthy bitches after parturition induced a transient increase in serum prolactin concentration and stimulated milk lactose production.
29359978	10	9	dep	treatment	1402:1410	arg1	bitches					1424:1430	bitches	1424:1430	bitches	1424:1430	Milk dry matter content was unchanged, whereas the time course for milk gross energy content differed significantly between treatment and control bitches.
29359978	10	10	theme	time	1329:1332	arg1	course					1334:1339	the time course	1325:1339	the time course for milk gross energy content	1325:1369	Milk dry matter content was unchanged, whereas the time course for milk gross energy content differed significantly between treatment and control bitches.
29359978	0	11	theme	serum	84:88	arg1	concentration					100:112	serum prolactin concentration	84:112	serum prolactin concentration	84:112	Effect of metoclopramide treatment of bitches during the first week of lactation on serum prolactin concentration, milk composition, and milk yield and on weight gain of their puppies.
29359978	3	12	theme	last	608:611	arg1	puppy					613:617	the last puppy	604:617	the last puppy of the litter (day 0)	604:639	PROCEDURES 10 bitches received metoclopramide (0.2 mg/kg, PO, q 6 h for 6 days; treatment group) starting 10 to 24 hours after birth of the last puppy of the litter (day 0), and 10 bitches served as the control group.
29359978	7	13	theme	Serum	894:898	arg1	concentration					910:922	RESULTS Serum prolactin concentration	886:922	RESULTS Serum prolactin concentration	886:922	RESULTS Serum prolactin concentration increased significantly over time in both groups, and no treatment effect was detected.
29359978	12	14	theme	metoclopramide	1555:1568	arg1	administration					1537:1550	CONCLUSIONS AND CLINICAL RELEVANCE Oral administration	1497:1550	CONCLUSIONS AND CLINICAL RELEVANCE Oral administration of metoclopramide to healthy bitches after parturition	1497:1605	CONCLUSIONS AND CLINICAL RELEVANCE Oral administration of metoclopramide to healthy bitches after parturition induced a transient increase in serum prolactin concentration and stimulated milk lactose production.
29359978	7	15	theme	prolactin	900:908	arg1	concentration					910:922	RESULTS Serum prolactin concentration	886:922	RESULTS Serum prolactin concentration	886:922	RESULTS Serum prolactin concentration increased significantly over time in both groups, and no treatment effect was detected.
29359978	13	16	with	bitches	1722:1728	arg1	production					1764:1773	insufficient or delayed milk production	1735:1773	insufficient or delayed milk production	1735:1773	It is likely bitches with insufficient or delayed milk production could benefit from metoclopramide treatment.
29359978	8	17	theme	treatment	1116:1124	arg1	group					1126:1130	the treatment group	1112:1130	the treatment group	1112:1130	When day-to-day changes were analyzed, the prolactin concentration increased from day 0 to day 1 in the treatment group but not in the control group.
29359978	4	18	from	bitches	718:724	arg1	samples					701:707	Blood and milk samples	686:707	Blood and milk samples from all bitches	686:724	Blood and milk samples from all bitches were collected on days 0, 1, 2, 4, and 6.
29359978	0	19	theme	puppies	176:182	arg1	gain					162:165	weight gain	155:165	weight gain of their puppies	155:182	Effect of metoclopramide treatment of bitches during the first week of lactation on serum prolactin concentration, milk composition, and milk yield and on weight gain of their puppies.
29359978	12	20	theme	healthy	1573:1579	arg1	bitches					1581:1587	healthy bitches	1573:1587	healthy bitches	1573:1587	CONCLUSIONS AND CLINICAL RELEVANCE Oral administration of metoclopramide to healthy bitches after parturition induced a transient increase in serum prolactin concentration and stimulated milk lactose production.
29359978	12	21	theme	transient	1617:1625	arg1	increase					1627:1634	a transient increase	1615:1634	a transient increase in serum prolactin concentration	1615:1667	CONCLUSIONS AND CLINICAL RELEVANCE Oral administration of metoclopramide to healthy bitches after parturition induced a transient increase in serum prolactin concentration and stimulated milk lactose production.
29359978	0	22	theme	prolactin	90:98	arg1	concentration					100:112	serum prolactin concentration	84:112	serum prolactin concentration	84:112	Effect of metoclopramide treatment of bitches during the first week of lactation on serum prolactin concentration, milk composition, and milk yield and on weight gain of their puppies.
29359978	1	23	theme	dry	345:347	arg1	content					356:362	dry matter content	345:362	dry matter content	345:362	OBJECTIVE To investigate effects of metoclopramide orally administered to healthy bitches on serum prolactin and milk lactose concentrations, gross energy, and dry matter content and on puppy weight gain during early lactation.
29359978	3	24	dep	hours	583:587	arg1	birth					595:599	birth	595:599	birth of the last puppy of the litter (day 0)	595:639	PROCEDURES 10 bitches received metoclopramide (0.2 mg/kg, PO, q 6 h for 6 days; treatment group) starting 10 to 24 hours after birth of the last puppy of the litter (day 0), and 10 bitches served as the control group.
29359978	12	25	theme	Oral	1532:1535	arg1	administration					1537:1550	CONCLUSIONS AND CLINICAL RELEVANCE Oral administration	1497:1550	CONCLUSIONS AND CLINICAL RELEVANCE Oral administration of metoclopramide to healthy bitches after parturition	1497:1605	CONCLUSIONS AND CLINICAL RELEVANCE Oral administration of metoclopramide to healthy bitches after parturition induced a transient increase in serum prolactin concentration and stimulated milk lactose production.
29359978	1	26	theme	matter	349:354	arg1	content					356:362	dry matter content	345:362	dry matter content	345:362	OBJECTIVE To investigate effects of metoclopramide orally administered to healthy bitches on serum prolactin and milk lactose concentrations, gross energy, and dry matter content and on puppy weight gain during early lactation.
29359978	12	27	theme	RELEVANCE	1522:1530	arg1	administration					1537:1550	CONCLUSIONS AND CLINICAL RELEVANCE Oral administration	1497:1550	CONCLUSIONS AND CLINICAL RELEVANCE Oral administration of metoclopramide to healthy bitches after parturition	1497:1605	CONCLUSIONS AND CLINICAL RELEVANCE Oral administration of metoclopramide to healthy bitches after parturition induced a transient increase in serum prolactin concentration and stimulated milk lactose production.
29359978	3	28	theme	q	530:530	arg1	h					534:534	q 6 h	530:534	q 6 h for 6 days	530:545	PROCEDURES 10 bitches received metoclopramide (0.2 mg/kg, PO, q 6 h for 6 days; treatment group) starting 10 to 24 hours after birth of the last puppy of the litter (day 0), and 10 bitches served as the control group.
29359978	10	29	theme	energy	1356:1361	arg1	content					1363:1369	milk gross energy content	1345:1369	milk gross energy content	1345:1369	Milk dry matter content was unchanged, whereas the time course for milk gross energy content differed significantly between treatment and control bitches.
29359978	1	30	theme	healthy	259:265	arg1	bitches					267:273	healthy bitches	259:273	healthy bitches	259:273	OBJECTIVE To investigate effects of metoclopramide orally administered to healthy bitches on serum prolactin and milk lactose concentrations, gross energy, and dry matter content and on puppy weight gain during early lactation.
29359978	3	31	dep	24	580:581	arg1	to					577:578	to	577:578	to	577:578	PROCEDURES 10 bitches received metoclopramide (0.2 mg/kg, PO, q 6 h for 6 days; treatment group) starting 10 to 24 hours after birth of the last puppy of the litter (day 0), and 10 bitches served as the control group.
29359978	0	32	theme	treatment	25:33	arg1	Effect					0:5	Effect	0:5	Effect of metoclopramide treatment of bitches during the first week of lactation on serum prolactin concentration, milk composition, and milk yield and on weight gain of their puppies.	0:183	Effect of metoclopramide treatment of bitches during the first week of lactation on serum prolactin concentration, milk composition, and milk yield and on weight gain of their puppies.
29359978	0	33	theme	milk	115:118	arg1	composition					120:130	milk composition	115:130	milk composition	115:130	Effect of metoclopramide treatment of bitches during the first week of lactation on serum prolactin concentration, milk composition, and milk yield and on weight gain of their puppies.
29359978	3	34	dep	received	490:497	arg1	mg/kg					519:523	0.2 mg/kg	515:523	0.2 mg/kg	515:523	PROCEDURES 10 bitches received metoclopramide (0.2 mg/kg, PO, q 6 h for 6 days; treatment group) starting 10 to 24 hours after birth of the last puppy of the litter (day 0), and 10 bitches served as the control group.
29359978	3	34	dep	received	490:497	arg1	PO					526:527	PO	526:527	PO	526:527	PROCEDURES 10 bitches received metoclopramide (0.2 mg/kg, PO, q 6 h for 6 days; treatment group) starting 10 to 24 hours after birth of the last puppy of the litter (day 0), and 10 bitches served as the control group.
29359978	7	35	theme	RESULTS	886:892	arg1	concentration					910:922	RESULTS Serum prolactin concentration	886:922	RESULTS Serum prolactin concentration	886:922	RESULTS Serum prolactin concentration increased significantly over time in both groups, and no treatment effect was detected.
29359978	8	36	theme	prolactin	1055:1063	arg1	concentration					1065:1077	the prolactin concentration	1051:1077	the prolactin concentration	1051:1077	When day-to-day changes were analyzed, the prolactin concentration increased from day 0 to day 1 in the treatment group but not in the control group.
29359978	0	37	theme	metoclopramide	10:23	arg1	treatment					25:33	metoclopramide treatment	10:33	metoclopramide treatment of bitches	10:44	Effect of metoclopramide treatment of bitches during the first week of lactation on serum prolactin concentration, milk composition, and milk yield and on weight gain of their puppies.
29359978	12	38	theme	milk	1684:1687	arg1	production					1697:1706	milk lactose production	1684:1706	milk lactose production	1684:1706	CONCLUSIONS AND CLINICAL RELEVANCE Oral administration of metoclopramide to healthy bitches after parturition induced a transient increase in serum prolactin concentration and stimulated milk lactose production.
29359978	11	39	theme	metoclopramide	1471:1484	arg1	treatment					1486:1494	metoclopramide treatment	1471:1494	metoclopramide treatment	1471:1494	Puppy weight gain was not affected by metoclopramide treatment.
29359978	8	40	theme	control	1147:1153	arg1	group					1155:1159	the control group	1143:1159	the control group	1143:1159	When day-to-day changes were analyzed, the prolactin concentration increased from day 0 to day 1 in the treatment group but not in the control group.
29359978	0	41	theme	bitches	38:44	arg1	treatment					25:33	metoclopramide treatment	10:33	metoclopramide treatment of bitches	10:44	Effect of metoclopramide treatment of bitches during the first week of lactation on serum prolactin concentration, milk composition, and milk yield and on weight gain of their puppies.
29359978	1	42	theme	serum	278:282	arg1	prolactin					284:292	serum prolactin and milk lactose concentrations	278:324	prolactin	284:292	OBJECTIVE To investigate effects of metoclopramide orally administered to healthy bitches on serum prolactin and milk lactose concentrations, gross energy, and dry matter content and on puppy weight gain during early lactation.
29359978	10	43	theme	gross	1350:1354	arg1	content					1363:1369	milk gross energy content	1345:1369	milk gross energy content	1345:1369	Milk dry matter content was unchanged, whereas the time course for milk gross energy content differed significantly between treatment and control bitches.
29359978	9	44	theme	treatment	1235:1243	arg1	group					1245:1249	the treatment group	1231:1249	the treatment group	1231:1249	Milk lactose concentration increased significantly and was higher in the treatment group than in the control group.
29359978	0	45	from	week	63:66	arg1	gain					162:165	weight gain	155:165	weight gain of their puppies	155:182	Effect of metoclopramide treatment of bitches during the first week of lactation on serum prolactin concentration, milk composition, and milk yield and on weight gain of their puppies.
29359978	0	45	from	week	63:66	arg1	concentration					100:112	serum prolactin concentration	84:112	serum prolactin concentration	84:112	Effect of metoclopramide treatment of bitches during the first week of lactation on serum prolactin concentration, milk composition, and milk yield and on weight gain of their puppies.
29359978	4	46	theme	Blood	686:690	arg1	samples					701:707	Blood and milk samples	686:707	Blood and milk samples from all bitches	686:724	Blood and milk samples from all bitches were collected on days 0, 1, 2, 4, and 6.
29359978	7	47	theme	treatment	981:989	arg1	effect					991:996	no treatment effect	978:996	no treatment effect	978:996	RESULTS Serum prolactin concentration increased significantly over time in both groups, and no treatment effect was detected.
29359978	8	48	theme	day-to-day	1017:1026	arg1	changes					1028:1034	day-to-day changes	1017:1034	day-to-day changes	1017:1034	When day-to-day changes were analyzed, the prolactin concentration increased from day 0 to day 1 in the treatment group but not in the control group.
29359978	10	49	theme	milk	1345:1348	arg1	content					1363:1369	milk gross energy content	1345:1369	milk gross energy content	1345:1369	Milk dry matter content was unchanged, whereas the time course for milk gross energy content differed significantly between treatment and control bitches.
29359978	0	50	theme	milk	137:140	arg1	yield					142:146	milk yield	137:146	milk yield	137:146	Effect of metoclopramide treatment of bitches during the first week of lactation on serum prolactin concentration, milk composition, and milk yield and on weight gain of their puppies.
29359978	1	51	theme	puppy	371:375	arg1	gain					384:387	puppy weight gain	371:387	puppy weight gain during early lactation	371:410	OBJECTIVE To investigate effects of metoclopramide orally administered to healthy bitches on serum prolactin and milk lactose concentrations, gross energy, and dry matter content and on puppy weight gain during early lactation.
29359978	11	52	theme	weight	1439:1444	arg1	gain					1446:1449	Puppy weight gain	1433:1449	Puppy weight gain	1433:1449	Puppy weight gain was not affected by metoclopramide treatment.
29359978	9	53	theme	control	1263:1269	arg1	group					1271:1275	the control group	1259:1275	the control group	1259:1275	Milk lactose concentration increased significantly and was higher in the treatment group than in the control group.
29359978	1	54	theme	milk	298:301	arg1	concentrations					311:324	serum prolactin and milk lactose concentrations	278:324	concentrations	311:324	OBJECTIVE To investigate effects of metoclopramide orally administered to healthy bitches on serum prolactin and milk lactose concentrations, gross energy, and dry matter content and on puppy weight gain during early lactation.
29359978	10	55	theme	Milk	1278:1281	arg1	content					1294:1300	Milk dry matter content	1278:1300	Milk dry matter content	1278:1300	Milk dry matter content was unchanged, whereas the time course for milk gross energy content differed significantly between treatment and control bitches.
29359978	1	56	theme	weight	377:382	arg1	gain					384:387	puppy weight gain	371:387	puppy weight gain during early lactation	371:410	OBJECTIVE To investigate effects of metoclopramide orally administered to healthy bitches on serum prolactin and milk lactose concentrations, gross energy, and dry matter content and on puppy weight gain during early lactation.
29359978	4	57	theme	milk	696:699	arg1	samples					701:707	Blood and milk samples	686:707	Blood and milk samples from all bitches	686:724	Blood and milk samples from all bitches were collected on days 0, 1, 2, 4, and 6.
29359978	12	58	theme	serum	1639:1643	arg1	concentration					1655:1667	serum prolactin concentration	1639:1667	serum prolactin concentration	1639:1667	CONCLUSIONS AND CLINICAL RELEVANCE Oral administration of metoclopramide to healthy bitches after parturition induced a transient increase in serum prolactin concentration and stimulated milk lactose production.
29359978	3	59	theme	PROCEDURES	468:477	arg1	bitches					482:488	PROCEDURES 10 bitches	468:488	PROCEDURES 10 bitches	468:488	PROCEDURES 10 bitches received metoclopramide (0.2 mg/kg, PO, q 6 h for 6 days; treatment group) starting 10 to 24 hours after birth of the last puppy of the litter (day 0), and 10 bitches served as the control group.
29359978	12	60	theme	CONCLUSIONS	1497:1507	arg1	administration					1537:1550	CONCLUSIONS AND CLINICAL RELEVANCE Oral administration	1497:1550	CONCLUSIONS AND CLINICAL RELEVANCE Oral administration of metoclopramide to healthy bitches after parturition	1497:1605	CONCLUSIONS AND CLINICAL RELEVANCE Oral administration of metoclopramide to healthy bitches after parturition induced a transient increase in serum prolactin concentration and stimulated milk lactose production.
29359978	1	61	theme	lactose	303:309	arg1	concentrations					311:324	serum prolactin and milk lactose concentrations	278:324	concentrations	311:324	OBJECTIVE To investigate effects of metoclopramide orally administered to healthy bitches on serum prolactin and milk lactose concentrations, gross energy, and dry matter content and on puppy weight gain during early lactation.
29359978	10	62	theme	matter	1287:1292	arg1	content					1294:1300	Milk dry matter content	1278:1300	Milk dry matter content	1278:1300	Milk dry matter content was unchanged, whereas the time course for milk gross energy content differed significantly between treatment and control bitches.
29359978	13	63	theme	milk	1759:1762	arg1	production					1764:1773	insufficient or delayed milk production	1735:1773	insufficient or delayed milk production	1735:1773	It is likely bitches with insufficient or delayed milk production could benefit from metoclopramide treatment.
29359978	13	64	theme	metoclopramide	1794:1807	arg1	treatment					1809:1817	metoclopramide treatment	1794:1817	metoclopramide treatment	1794:1817	It is likely bitches with insufficient or delayed milk production could benefit from metoclopramide treatment.
29359978	3	65	dep	mg/kg	519:523	arg1	h					534:534	q 6 h	530:534	q 6 h for 6 days	530:545	PROCEDURES 10 bitches received metoclopramide (0.2 mg/kg, PO, q 6 h for 6 days; treatment group) starting 10 to 24 hours after birth of the last puppy of the litter (day 0), and 10 bitches served as the control group.
29359978	3	65	dep	mg/kg	519:523	arg1	group					558:562	treatment group	548:562	treatment group	548:562	PROCEDURES 10 bitches received metoclopramide (0.2 mg/kg, PO, q 6 h for 6 days; treatment group) starting 10 to 24 hours after birth of the last puppy of the litter (day 0), and 10 bitches served as the control group.
29359978	5	66	theme	Milk	768:771	arg1	samples					773:779	Milk samples	768:779	Milk samples for days 1 and 2 and days 4 and 6	768:813	Milk samples for days 1 and 2 and days 4 and 6 were pooled because of small volume.
29359978	10	67	theme	dry	1283:1285	arg1	content					1294:1300	Milk dry matter content	1278:1300	Milk dry matter content	1278:1300	Milk dry matter content was unchanged, whereas the time course for milk gross energy content differed significantly between treatment and control bitches.
29359978	5	68	theme	small	838:842	arg1	volume					844:849	small volume	838:849	small volume	838:849	Milk samples for days 1 and 2 and days 4 and 6 were pooled because of small volume.
29359978	12	69	theme	lactose	1689:1695	arg1	production					1697:1706	milk lactose production	1684:1706	milk lactose production	1684:1706	CONCLUSIONS AND CLINICAL RELEVANCE Oral administration of metoclopramide to healthy bitches after parturition induced a transient increase in serum prolactin concentration and stimulated milk lactose production.
29359978	12	70	theme	CLINICAL	1513:1520	arg1	RELEVANCE					1522:1530	CLINICAL RELEVANCE	1513:1530	CLINICAL RELEVANCE	1513:1530	CONCLUSIONS AND CLINICAL RELEVANCE Oral administration of metoclopramide to healthy bitches after parturition induced a transient increase in serum prolactin concentration and stimulated milk lactose production.
29359978	9	71	theme	Milk	1162:1165	arg1	concentration					1175:1187	Milk lactose concentration	1162:1187	Milk lactose concentration	1162:1187	Milk lactose concentration increased significantly and was higher in the treatment group than in the control group.
29359978	12	72	theme	prolactin	1645:1653	arg1	concentration					1655:1667	serum prolactin concentration	1639:1667	serum prolactin concentration	1639:1667	CONCLUSIONS AND CLINICAL RELEVANCE Oral administration of metoclopramide to healthy bitches after parturition induced a transient increase in serum prolactin concentration and stimulated milk lactose production.
29359978	1	73	theme	early	396:400	arg1	lactation					402:410	early lactation	396:410	early lactation	396:410	OBJECTIVE To investigate effects of metoclopramide orally administered to healthy bitches on serum prolactin and milk lactose concentrations, gross energy, and dry matter content and on puppy weight gain during early lactation.
29359978	13	74	theme	delayed	1751:1757	arg1	production					1764:1773	insufficient or delayed milk production	1735:1773	insufficient or delayed milk production	1735:1773	It is likely bitches with insufficient or delayed milk production could benefit from metoclopramide treatment.
29359978	13	75	theme	insufficient	1735:1746	arg1	production					1764:1773	insufficient or delayed milk production	1735:1773	insufficient or delayed milk production	1735:1773	It is likely bitches with insufficient or delayed milk production could benefit from metoclopramide treatment.
29359978	9	76	theme	lactose	1167:1173	arg1	concentration					1175:1187	Milk lactose concentration	1162:1187	Milk lactose concentration	1162:1187	Milk lactose concentration increased significantly and was higher in the treatment group than in the control group.
29359978	0	77	theme	first	57:61	arg1	week					63:66	the first week	53:66	the first week of lactation on serum prolactin concentration, milk composition, and milk yield and on weight gain of their puppies	53:182	Effect of metoclopramide treatment of bitches during the first week of lactation on serum prolactin concentration, milk composition, and milk yield and on weight gain of their puppies.
29359978	3	78	theme	puppy	613:617	arg1	birth					595:599	birth	595:599	birth of the last puppy of the litter (day 0)	595:639	PROCEDURES 10 bitches received metoclopramide (0.2 mg/kg, PO, q 6 h for 6 days; treatment group) starting 10 to 24 hours after birth of the last puppy of the litter (day 0), and 10 bitches served as the control group.
29359978	3	79	theme	treatment	548:556	arg1	group					558:562	treatment group	548:562	treatment group	548:562	PROCEDURES 10 bitches received metoclopramide (0.2 mg/kg, PO, q 6 h for 6 days; treatment group) starting 10 to 24 hours after birth of the last puppy of the litter (day 0), and 10 bitches served as the control group.
29359978	3	80	theme	control	671:677	arg1	group					679:683	the control group	667:683	the control group	667:683	PROCEDURES 10 bitches received metoclopramide (0.2 mg/kg, PO, q 6 h for 6 days; treatment group) starting 10 to 24 hours after birth of the last puppy of the litter (day 0), and 10 bitches served as the control group.
29359978	3	80	theme	control	671:677	arg1	bitches					649:655	10 bitches	646:655	10 bitches	646:655	PROCEDURES 10 bitches received metoclopramide (0.2 mg/kg, PO, q 6 h for 6 days; treatment group) starting 10 to 24 hours after birth of the last puppy of the litter (day 0), and 10 bitches served as the control group.
30461376	4	0	contain	containing	455:464	arg1	7.24 Mb					446:452	7.24 Mb	446:452	7.24 Mb	446:452	The genome size of DXL2T was 7.24 Mb, containing 6243 predicted protein-coding genes, with a DNA G+C content of 60.2 mol%.
30461376	4	0	contain	containing	455:464	arg2	genes					496:500	6243 predicted protein-coding genes	466:500	6243 predicted protein-coding genes	466:500	The genome size of DXL2T was 7.24 Mb, containing 6243 predicted protein-coding genes, with a DNA G+C content of 60.2 mol%.
30461376	4	0	contain	containing	455:464	arg1	size					428:431	The genome size	417:431	The genome size of DXL2T	417:440	The genome size of DXL2T was 7.24 Mb, containing 6243 predicted protein-coding genes, with a DNA G+C content of 60.2 mol%.
30461376	8	1	theme	polar	736:740	arg1	diphosphatidylglycerol					754:775	diphosphatidylglycerol	754:775	diphosphatidylglycerol	754:775	The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two aminophospholipids, an unidentified aminolipid, phosphatidylmethylethanolamine, an unidentified glycolipid and an unidentified phospholipid.
30461376	8	1	theme	polar	736:740	arg1	lipids					742:747	The polar lipids	732:747	The polar lipids	732:747	The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two aminophospholipids, an unidentified aminolipid, phosphatidylmethylethanolamine, an unidentified glycolipid and an unidentified phospholipid.
30461376	4	2	theme	protein-coding	481:494	arg1	genes					496:500	6243 predicted protein-coding genes	466:500	6243 predicted protein-coding genes	466:500	The genome size of DXL2T was 7.24 Mb, containing 6243 predicted protein-coding genes, with a DNA G+C content of 60.2 mol%.
30461376	10	3	theme	phylogenetic	1185:1196	arg1	results					1198:1204	the phenotypic, chemotaxonomic and phylogenetic results	1150:1204	the phenotypic, chemotaxonomic and phylogenetic results	1150:1204	On the basis of the phenotypic, chemotaxonomic and phylogenetic results, strain DXL2T represents a novel species within the genus Paenibacillus, for which the name Paenibacillusflagellatus sp.
30461376	5	4	theme	meso-diaminopimelic	556:574	arg1	acid					576:579	meso-diaminopimelic acid	556:579	meso-diaminopimelic acid in the cell-wall peptidoglycan	556:610	DXL2T contained meso-diaminopimelic acid in the cell-wall peptidoglycan.
30461376	5	5	from	acid	576:579	arg1	peptidoglycan					598:610	the cell-wall peptidoglycan	584:610	the cell-wall peptidoglycan	584:610	DXL2T contained meso-diaminopimelic acid in the cell-wall peptidoglycan.
30461376	3	6	contain	had	215:217	arg1	DXL2T					209:213	DXL2T	209:213	DXL2T	209:213	DXL2T had the highest 16S rRNA gene sequence similarities with those of Paenibacillus ginsengarviGsoil 139T (96.8 %), Paenibacillushemerocallicola DLE-12T (95.5 %) and Paenibacillus hodogayensisSGT (95.4 %).
30461376	3	6	contain	had	215:217	arg2	similarities					254:265	the highest 16S rRNA gene sequence similarities	219:265	the highest 16S rRNA gene sequence similarities	219:265	DXL2T had the highest 16S rRNA gene sequence similarities with those of Paenibacillus ginsengarviGsoil 139T (96.8 %), Paenibacillushemerocallicola DLE-12T (95.5 %) and Paenibacillus hodogayensisSGT (95.4 %).
30461376	4	7	theme	%	537:537	arg1	G+C content					514:524	a DNA G+C content	508:524	a DNA G+C content of 60.2 mol%	508:537	The genome size of DXL2T was 7.24 Mb, containing 6243 predicted protein-coding genes, with a DNA G+C content of 60.2 mol%.
30461376	3	8	theme	Paenibacillushemerocallicola	327:354	arg1	%					370:370	95.5 %	365:370	95.5 %	365:370	DXL2T had the highest 16S rRNA gene sequence similarities with those of Paenibacillus ginsengarviGsoil 139T (96.8 %), Paenibacillushemerocallicola DLE-12T (95.5 %) and Paenibacillus hodogayensisSGT (95.4 %).
30461376	3	8	theme	Paenibacillushemerocallicola	327:354	arg1	DLE-12T					356:362	Paenibacillushemerocallicola DLE-12T	327:362	Paenibacillushemerocallicola DLE-12T (95.5 %)	327:371	DXL2T had the highest 16S rRNA gene sequence similarities with those of Paenibacillus ginsengarviGsoil 139T (96.8 %), Paenibacillushemerocallicola DLE-12T (95.5 %) and Paenibacillus hodogayensisSGT (95.4 %).
30461376	2	9	theme	Gram-stain-negative	89:107	arg1	DXL2T					80:84	Strain DXL2T	73:84	Strain DXL2T	73:84	Strain DXL2T, a Gram-stain-negative, rod-shaped, endospore-forming, motile, aerobic bacterium, was isolated from selenium mineral soil.
30461376	2	9	theme	Gram-stain-negative	89:107	arg1	bacterium					157:165	a Gram-stain-negative, rod-shaped, endospore-forming, motile, aerobic bacterium	87:165	a Gram-stain-negative, rod-shaped, endospore-forming, motile, aerobic bacterium	87:165	Strain DXL2T, a Gram-stain-negative, rod-shaped, endospore-forming, motile, aerobic bacterium, was isolated from selenium mineral soil.
30461376	1	10	theme	selenium	50:57	arg1	soil					67:70	selenium mineral soil	50:70	selenium mineral soil	50:70	nov., isolated from selenium mineral soil.
30461376	12	11	theme	=KCTC	1371:1375	arg1	2018054T					1393:1400	=KCTC 33976T=CCTCC AB 2018054T	1371:1400	=KCTC 33976T=CCTCC AB 2018054T	1371:1400	The type strain is DXL2T (=KCTC 33976T=CCTCC AB 2018054T).
30461376	12	11	theme	=KCTC	1371:1375	arg1	DXL2T					1364:1368	DXL2T	1364:1368	DXL2T (=KCTC 33976T=CCTCC AB 2018054T)	1364:1401	The type strain is DXL2T (=KCTC 33976T=CCTCC AB 2018054T).
30461376	12	12	theme	AB	1390:1391	arg1	2018054T					1393:1400	=KCTC 33976T=CCTCC AB 2018054T	1371:1400	=KCTC 33976T=CCTCC AB 2018054T	1371:1400	The type strain is DXL2T (=KCTC 33976T=CCTCC AB 2018054T).
30461376	12	12	theme	AB	1390:1391	arg1	DXL2T					1364:1368	DXL2T	1364:1368	DXL2T (=KCTC 33976T=CCTCC AB 2018054T)	1364:1401	The type strain is DXL2T (=KCTC 33976T=CCTCC AB 2018054T).
30461376	1	13	theme	mineral	59:65	arg1	soil					67:70	selenium mineral soil	50:70	selenium mineral soil	50:70	nov., isolated from selenium mineral soil.
30461376	4	14	theme	DXL2T	436:440	arg1	7.24 Mb					446:452	7.24 Mb	446:452	7.24 Mb	446:452	The genome size of DXL2T was 7.24 Mb, containing 6243 predicted protein-coding genes, with a DNA G+C content of 60.2 mol%.
30461376	4	14	theme	DXL2T	436:440	arg1	size					428:431	The genome size	417:431	The genome size of DXL2T	417:440	The genome size of DXL2T was 7.24 Mb, containing 6243 predicted protein-coding genes, with a DNA G+C content of 60.2 mol%.
30461376	3	15	theme	16S	231:233	arg1	rRNA					235:238	the highest 16S rRNA	219:238	the highest 16S rRNA gene sequence similarities	219:265	DXL2T had the highest 16S rRNA gene sequence similarities with those of Paenibacillus ginsengarviGsoil 139T (96.8 %), Paenibacillushemerocallicola DLE-12T (95.5 %) and Paenibacillus hodogayensisSGT (95.4 %).
30461376	3	16	theme	rRNA	235:238	arg1	similarities					254:265	the highest 16S rRNA gene sequence similarities	219:265	the highest 16S rRNA gene sequence similarities	219:265	DXL2T had the highest 16S rRNA gene sequence similarities with those of Paenibacillus ginsengarviGsoil 139T (96.8 %), Paenibacillushemerocallicola DLE-12T (95.5 %) and Paenibacillus hodogayensisSGT (95.4 %).
30461376	0	17	theme	flagellatus	14:24	arg1	sp					26:27	Paenibacillus flagellatus sp	0:27	Paenibacillus flagellatus sp.	0:28	Paenibacillus flagellatus sp.
30461376	8	18	theme	unidentified	853:864	arg1	aminolipid					866:875	an unidentified aminolipid	850:875	an unidentified aminolipid	850:875	The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two aminophospholipids, an unidentified aminolipid, phosphatidylmethylethanolamine, an unidentified glycolipid and an unidentified phospholipid.
30461376	2	19	attach	isolated	172:179	arg2	DXL2T					80:84	Strain DXL2T	73:84	Strain DXL2T	73:84	Strain DXL2T, a Gram-stain-negative, rod-shaped, endospore-forming, motile, aerobic bacterium, was isolated from selenium mineral soil.
30461376	2	19	attach	isolated	172:179	arg1	soil					203:206	selenium mineral soil	186:206	selenium mineral soil	186:206	Strain DXL2T, a Gram-stain-negative, rod-shaped, endospore-forming, motile, aerobic bacterium, was isolated from selenium mineral soil.
30461376	2	19	attach	isolated	172:179	arg2	bacterium					157:165	a Gram-stain-negative, rod-shaped, endospore-forming, motile, aerobic bacterium	87:165	a Gram-stain-negative, rod-shaped, endospore-forming, motile, aerobic bacterium	87:165	Strain DXL2T, a Gram-stain-negative, rod-shaped, endospore-forming, motile, aerobic bacterium, was isolated from selenium mineral soil.
30461376	0	20	theme	Paenibacillus	0:12	arg1	sp					26:27	Paenibacillus flagellatus sp	0:27	Paenibacillus flagellatus sp.	0:28	Paenibacillus flagellatus sp.
30461376	3	21	theme	highest	223:229	arg1	rRNA					235:238	the highest 16S rRNA	219:238	the highest 16S rRNA gene sequence similarities	219:265	DXL2T had the highest 16S rRNA gene sequence similarities with those of Paenibacillus ginsengarviGsoil 139T (96.8 %), Paenibacillushemerocallicola DLE-12T (95.5 %) and Paenibacillus hodogayensisSGT (95.4 %).
30461376	5	22	theme	cell-wall	588:596	arg1	peptidoglycan					598:610	the cell-wall peptidoglycan	584:610	the cell-wall peptidoglycan	584:610	DXL2T contained meso-diaminopimelic acid in the cell-wall peptidoglycan.
30461376	10	23	dep	results	1198:1204	arg1	the					1137:1139	the	1137:1139	the	1137:1139	On the basis of the phenotypic, chemotaxonomic and phylogenetic results, strain DXL2T represents a novel species within the genus Paenibacillus, for which the name Paenibacillusflagellatus sp.
30461376	10	23	dep	results	1198:1204	arg1	basis					1141:1145	basis	1141:1145	basis	1141:1145	On the basis of the phenotypic, chemotaxonomic and phylogenetic results, strain DXL2T represents a novel species within the genus Paenibacillus, for which the name Paenibacillusflagellatus sp.
30461376	9	24	theme	other	989:993	arg1	strains					995:1001	the other strains	985:1001	the other strains	985:1001	Compared with the other strains, DXL2T had a specific phospholipid and a specific aminolipid, it hydrolyzed Tween 40 and could not assimilate potassium gluconate.
30461376	4	25	theme	DNA	510:512	arg1	G+C content					514:524	a DNA G+C content	508:524	a DNA G+C content of 60.2 mol%	508:537	The genome size of DXL2T was 7.24 Mb, containing 6243 predicted protein-coding genes, with a DNA G+C content of 60.2 mol%.
30461376	12	26	theme	33976T=CCTCC	1377:1388	arg1	2018054T					1393:1400	=KCTC 33976T=CCTCC AB 2018054T	1371:1400	=KCTC 33976T=CCTCC AB 2018054T	1371:1400	The type strain is DXL2T (=KCTC 33976T=CCTCC AB 2018054T).
30461376	12	26	theme	33976T=CCTCC	1377:1388	arg1	DXL2T					1364:1368	DXL2T	1364:1368	DXL2T (=KCTC 33976T=CCTCC AB 2018054T)	1364:1401	The type strain is DXL2T (=KCTC 33976T=CCTCC AB 2018054T).
30461376	6	27	dep	anteiso-C15 	649:660	arg1	 0					691:692	 0	691:692	 0	691:692	The major cellular fatty acids were anteiso-C15 : 0, iso-C16 : 0 and iso-C15 : 0.
30461376	6	27	dep	anteiso-C15 	649:660	arg1	 0					662:663	 0	662:663	 0	662:663	The major cellular fatty acids were anteiso-C15 : 0, iso-C16 : 0 and iso-C15 : 0.
30461376	6	27	dep	anteiso-C15 	649:660	arg1	iso-C15 					682:689	iso-C15 	682:689	iso-C15 	682:689	The major cellular fatty acids were anteiso-C15 : 0, iso-C16 : 0 and iso-C15 : 0.
30461376	6	27	dep	anteiso-C15 	649:660	arg1	 0					675:676	 0	675:676	 0	675:676	The major cellular fatty acids were anteiso-C15 : 0, iso-C16 : 0 and iso-C15 : 0.
30461376	5	28	contain	contained	546:554	arg1	DXL2T					540:544	DXL2T	540:544	DXL2T	540:544	DXL2T contained meso-diaminopimelic acid in the cell-wall peptidoglycan.
30461376	5	28	contain	contained	546:554	arg2	acid					576:579	meso-diaminopimelic acid	556:579	meso-diaminopimelic acid in the cell-wall peptidoglycan	556:610	DXL2T contained meso-diaminopimelic acid in the cell-wall peptidoglycan.
30461376	4	29	theme	60.2 mol	529:536	arg1	%					537:537	60.2 mol%	529:537	60.2 mol%	529:537	The genome size of DXL2T was 7.24 Mb, containing 6243 predicted protein-coding genes, with a DNA G+C content of 60.2 mol%.
30461376	4	30	theme	genome	421:426	arg1	7.24 Mb					446:452	7.24 Mb	446:452	7.24 Mb	446:452	The genome size of DXL2T was 7.24 Mb, containing 6243 predicted protein-coding genes, with a DNA G+C content of 60.2 mol%.
30461376	4	30	theme	genome	421:426	arg1	size					428:431	The genome size	417:431	The genome size of DXL2T	417:440	The genome size of DXL2T was 7.24 Mb, containing 6243 predicted protein-coding genes, with a DNA G+C content of 60.2 mol%.
30461376	10	31	theme	name	1293:1296	arg1	sp					1323:1324	the name Paenibacillusflagellatus sp	1289:1324	the name Paenibacillusflagellatus sp	1289:1324	On the basis of the phenotypic, chemotaxonomic and phylogenetic results, strain DXL2T represents a novel species within the genus Paenibacillus, for which the name Paenibacillusflagellatus sp.
30461376	10	32	theme	Paenibacillusflagellatus	1298:1321	arg1	sp					1323:1324	the name Paenibacillusflagellatus sp	1289:1324	the name Paenibacillusflagellatus sp	1289:1324	On the basis of the phenotypic, chemotaxonomic and phylogenetic results, strain DXL2T represents a novel species within the genus Paenibacillus, for which the name Paenibacillusflagellatus sp.
30461376	3	33	theme	gene	240:243	arg1	similarities					254:265	the highest 16S rRNA gene sequence similarities	219:265	the highest 16S rRNA gene sequence similarities	219:265	DXL2T had the highest 16S rRNA gene sequence similarities with those of Paenibacillus ginsengarviGsoil 139T (96.8 %), Paenibacillushemerocallicola DLE-12T (95.5 %) and Paenibacillus hodogayensisSGT (95.4 %).
30461376	10	34	theme	strain	1207:1212	arg1	DXL2T					1214:1218	strain DXL2T	1207:1218	strain DXL2T	1207:1218	On the basis of the phenotypic, chemotaxonomic and phylogenetic results, strain DXL2T represents a novel species within the genus Paenibacillus, for which the name Paenibacillusflagellatus sp.
30461376	12	35	theme	type	1349:1352	arg1	DXL2T					1364:1368	DXL2T	1364:1368	DXL2T (=KCTC 33976T=CCTCC AB 2018054T)	1364:1401	The type strain is DXL2T (=KCTC 33976T=CCTCC AB 2018054T).
30461376	12	35	theme	type	1349:1352	arg1	strain					1354:1359	The type strain	1345:1359	The type strain	1345:1359	The type strain is DXL2T (=KCTC 33976T=CCTCC AB 2018054T).
30461376	9	36	theme	potassium	1113:1121	arg1	gluconate					1123:1131	potassium gluconate	1113:1131	potassium gluconate	1113:1131	Compared with the other strains, DXL2T had a specific phospholipid and a specific aminolipid, it hydrolyzed Tween 40 and could not assimilate potassium gluconate.
30461376	10	37	theme	phenotypic	1154:1163	arg1	results					1198:1204	the phenotypic, chemotaxonomic and phylogenetic results	1150:1204	the phenotypic, chemotaxonomic and phylogenetic results	1150:1204	On the basis of the phenotypic, chemotaxonomic and phylogenetic results, strain DXL2T represents a novel species within the genus Paenibacillus, for which the name Paenibacillusflagellatus sp.
30461376	3	38	theme	sequence	245:252	arg1	similarities					254:265	the highest 16S rRNA gene sequence similarities	219:265	the highest 16S rRNA gene sequence similarities	219:265	DXL2T had the highest 16S rRNA gene sequence similarities with those of Paenibacillus ginsengarviGsoil 139T (96.8 %), Paenibacillushemerocallicola DLE-12T (95.5 %) and Paenibacillus hodogayensisSGT (95.4 %).
30461376	2	39	dep	Gram-stain-negative	89:107	arg1	rod-shaped					110:119	rod-shaped	110:119	rod-shaped	110:119	Strain DXL2T, a Gram-stain-negative, rod-shaped, endospore-forming, motile, aerobic bacterium, was isolated from selenium mineral soil.
30461376	2	39	dep	Gram-stain-negative	89:107	arg1	aerobic					149:155	aerobic	149:155	aerobic	149:155	Strain DXL2T, a Gram-stain-negative, rod-shaped, endospore-forming, motile, aerobic bacterium, was isolated from selenium mineral soil.
30461376	2	39	dep	Gram-stain-negative	89:107	arg1	motile					141:146	motile	141:146	motile	141:146	Strain DXL2T, a Gram-stain-negative, rod-shaped, endospore-forming, motile, aerobic bacterium, was isolated from selenium mineral soil.
30461376	2	39	dep	Gram-stain-negative	89:107	arg1	endospore-forming					122:138	endospore-forming	122:138	endospore-forming	122:138	Strain DXL2T, a Gram-stain-negative, rod-shaped, endospore-forming, motile, aerobic bacterium, was isolated from selenium mineral soil.
30461376	3	40	theme	Paenibacillus	281:293	arg1	139T					312:315	Paenibacillus ginsengarviGsoil 139T	281:315	Paenibacillus ginsengarviGsoil 139T (96.8 %)	281:324	DXL2T had the highest 16S rRNA gene sequence similarities with those of Paenibacillus ginsengarviGsoil 139T (96.8 %), Paenibacillushemerocallicola DLE-12T (95.5 %) and Paenibacillus hodogayensisSGT (95.4 %).
30461376	3	40	theme	Paenibacillus	281:293	arg1	%					323:323	96.8 %	318:323	96.8 %	318:323	DXL2T had the highest 16S rRNA gene sequence similarities with those of Paenibacillus ginsengarviGsoil 139T (96.8 %), Paenibacillushemerocallicola DLE-12T (95.5 %) and Paenibacillus hodogayensisSGT (95.4 %).
30461376	8	41	theme	unidentified	913:924	arg1	glycolipid					926:935	an unidentified glycolipid	910:935	an unidentified glycolipid	910:935	The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two aminophospholipids, an unidentified aminolipid, phosphatidylmethylethanolamine, an unidentified glycolipid and an unidentified phospholipid.
30461376	10	42	theme	Paenibacillus	1264:1276	arg1	genus					1258:1262	the genus Paenibacillus	1254:1276	the genus Paenibacillus	1254:1276	On the basis of the phenotypic, chemotaxonomic and phylogenetic results, strain DXL2T represents a novel species within the genus Paenibacillus, for which the name Paenibacillusflagellatus sp.
30461376	3	43	theme	Paenibacillus	377:389	arg1	hodogayensisSGT					391:405	Paenibacillus hodogayensisSGT	377:405	Paenibacillus hodogayensisSGT (95.4 %)	377:414	DXL2T had the highest 16S rRNA gene sequence similarities with those of Paenibacillus ginsengarviGsoil 139T (96.8 %), Paenibacillushemerocallicola DLE-12T (95.5 %) and Paenibacillus hodogayensisSGT (95.4 %).
30461376	3	43	theme	Paenibacillus	377:389	arg1	%					413:413	95.4 %	408:413	95.4 %	408:413	DXL2T had the highest 16S rRNA gene sequence similarities with those of Paenibacillus ginsengarviGsoil 139T (96.8 %), Paenibacillushemerocallicola DLE-12T (95.5 %) and Paenibacillus hodogayensisSGT (95.4 %).
30461376	3	44	theme	ginsengarviGsoil	295:310	arg1	139T					312:315	Paenibacillus ginsengarviGsoil 139T	281:315	Paenibacillus ginsengarviGsoil 139T (96.8 %)	281:324	DXL2T had the highest 16S rRNA gene sequence similarities with those of Paenibacillus ginsengarviGsoil 139T (96.8 %), Paenibacillushemerocallicola DLE-12T (95.5 %) and Paenibacillus hodogayensisSGT (95.4 %).
30461376	3	44	theme	ginsengarviGsoil	295:310	arg1	%					323:323	96.8 %	318:323	96.8 %	318:323	DXL2T had the highest 16S rRNA gene sequence similarities with those of Paenibacillus ginsengarviGsoil 139T (96.8 %), Paenibacillushemerocallicola DLE-12T (95.5 %) and Paenibacillus hodogayensisSGT (95.4 %).
30461376	2	45	theme	mineral	195:201	arg1	soil					203:206	selenium mineral soil	186:206	selenium mineral soil	186:206	Strain DXL2T, a Gram-stain-negative, rod-shaped, endospore-forming, motile, aerobic bacterium, was isolated from selenium mineral soil.
30461376	9	46	theme	specific	1016:1023	arg1	phospholipid					1025:1036	a specific phospholipid	1014:1036	a specific phospholipid	1014:1036	Compared with the other strains, DXL2T had a specific phospholipid and a specific aminolipid, it hydrolyzed Tween 40 and could not assimilate potassium gluconate.
30461376	2	47	theme	Strain	73:78	arg1	DXL2T					80:84	Strain DXL2T	73:84	Strain DXL2T	73:84	Strain DXL2T, a Gram-stain-negative, rod-shaped, endospore-forming, motile, aerobic bacterium, was isolated from selenium mineral soil.
30461376	2	47	theme	Strain	73:78	arg1	bacterium					157:165	a Gram-stain-negative, rod-shaped, endospore-forming, motile, aerobic bacterium	87:165	a Gram-stain-negative, rod-shaped, endospore-forming, motile, aerobic bacterium	87:165	Strain DXL2T, a Gram-stain-negative, rod-shaped, endospore-forming, motile, aerobic bacterium, was isolated from selenium mineral soil.
30461376	2	48	theme	selenium	186:193	arg1	soil					203:206	selenium mineral soil	186:206	selenium mineral soil	186:206	Strain DXL2T, a Gram-stain-negative, rod-shaped, endospore-forming, motile, aerobic bacterium, was isolated from selenium mineral soil.
30461376	10	49	theme	novel	1233:1237	arg1	species					1239:1245	a novel species	1231:1245	a novel species	1231:1245	On the basis of the phenotypic, chemotaxonomic and phylogenetic results, strain DXL2T represents a novel species within the genus Paenibacillus, for which the name Paenibacillusflagellatus sp.
30461376	6	50	theme	fatty	632:636	arg1	anteiso-C15 					649:660	anteiso-C15 	649:660	anteiso-C15 	649:660	The major cellular fatty acids were anteiso-C15 : 0, iso-C16 : 0 and iso-C15 : 0.
30461376	6	50	theme	fatty	632:636	arg1	acids					638:642	The major cellular fatty acids	613:642	The major cellular fatty acids	613:642	The major cellular fatty acids were anteiso-C15 : 0, iso-C16 : 0 and iso-C15 : 0.
30461376	7	51	theme	major	699:703	arg1	menaquinone					717:727	menaquinone 7	717:729	menaquinone 7	717:729	The major quinone was menaquinone 7.
30461376	7	51	theme	major	699:703	arg1	quinone					705:711	The major quinone	695:711	The major quinone	695:711	The major quinone was menaquinone 7.
30461376	4	52	theme	predicted	471:479	arg1	genes					496:500	6243 predicted protein-coding genes	466:500	6243 predicted protein-coding genes	466:500	The genome size of DXL2T was 7.24 Mb, containing 6243 predicted protein-coding genes, with a DNA G+C content of 60.2 mol%.
30461376	9	53	contain	had	1010:1012	arg1	DXL2T					1004:1008	DXL2T	1004:1008	DXL2T	1004:1008	Compared with the other strains, DXL2T had a specific phospholipid and a specific aminolipid, it hydrolyzed Tween 40 and could not assimilate potassium gluconate.
30461376	9	53	contain	had	1010:1012	arg2	aminolipid					1053:1062	a specific aminolipid	1042:1062	a specific aminolipid	1042:1062	Compared with the other strains, DXL2T had a specific phospholipid and a specific aminolipid, it hydrolyzed Tween 40 and could not assimilate potassium gluconate.
30461376	9	53	contain	had	1010:1012	arg2	phospholipid					1025:1036	a specific phospholipid	1014:1036	a specific phospholipid	1014:1036	Compared with the other strains, DXL2T had a specific phospholipid and a specific aminolipid, it hydrolyzed Tween 40 and could not assimilate potassium gluconate.
30461376	6	54	theme	cellular	623:630	arg1	anteiso-C15 					649:660	anteiso-C15 	649:660	anteiso-C15 	649:660	The major cellular fatty acids were anteiso-C15 : 0, iso-C16 : 0 and iso-C15 : 0.
30461376	6	54	theme	cellular	623:630	arg1	acids					638:642	The major cellular fatty acids	613:642	The major cellular fatty acids	613:642	The major cellular fatty acids were anteiso-C15 : 0, iso-C16 : 0 and iso-C15 : 0.
30461376	8	55	theme	unidentified	944:955	arg1	phospholipid					957:968	an unidentified phospholipid	941:968	an unidentified phospholipid	941:968	The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two aminophospholipids, an unidentified aminolipid, phosphatidylmethylethanolamine, an unidentified glycolipid and an unidentified phospholipid.
30461376	6	56	theme	major	617:621	arg1	anteiso-C15 					649:660	anteiso-C15 	649:660	anteiso-C15 	649:660	The major cellular fatty acids were anteiso-C15 : 0, iso-C16 : 0 and iso-C15 : 0.
30461376	6	56	theme	major	617:621	arg1	acids					638:642	The major cellular fatty acids	613:642	The major cellular fatty acids	613:642	The major cellular fatty acids were anteiso-C15 : 0, iso-C16 : 0 and iso-C15 : 0.
30461376	10	57	theme	chemotaxonomic	1166:1179	arg1	results					1198:1204	the phenotypic, chemotaxonomic and phylogenetic results	1150:1204	the phenotypic, chemotaxonomic and phylogenetic results	1150:1204	On the basis of the phenotypic, chemotaxonomic and phylogenetic results, strain DXL2T represents a novel species within the genus Paenibacillus, for which the name Paenibacillusflagellatus sp.
30461376	4	58	with	7.24 Mb	446:452	arg1	G+C content					514:524	a DNA G+C content	508:524	a DNA G+C content of 60.2 mol%	508:537	The genome size of DXL2T was 7.24 Mb, containing 6243 predicted protein-coding genes, with a DNA G+C content of 60.2 mol%.
30461376	9	59	theme	specific	1044:1051	arg1	aminolipid					1053:1062	a specific aminolipid	1042:1062	a specific aminolipid	1042:1062	Compared with the other strains, DXL2T had a specific phospholipid and a specific aminolipid, it hydrolyzed Tween 40 and could not assimilate potassium gluconate.
29458522	3	0	theme	peritrichous	291:302	arg1	flagella					304:311	peritrichous flagella	291:311	peritrichous flagella	291:311	Cells of strain S8T were endospore-forming, motile by means of peritrichous flagella, and rod-shaped.
29458522	6	1	theme	yonginensis	627:637	arg1	DCY84T					639:644	Paenibacillus yonginensis DCY84T	613:644	Paenibacillus yonginensis DCY84T	613:644	Based on 16S rRNA gene sequence similarity, strain S8T was affiliated with the genus Paenibacillus in the family Paenibacillaceae and was most closely related to Paenibacillus yonginensis DCY84T and Paenibacillus physcomitrellae XBT (98.8 and 97.1 % sequence similarity).
29458522	6	1	theme	yonginensis	627:637	arg1	similarity					710:719	98.8 and 97.1 % sequence similarity	685:719	98.8 and 97.1 % sequence similarity	685:719	Based on 16S rRNA gene sequence similarity, strain S8T was affiliated with the genus Paenibacillus in the family Paenibacillaceae and was most closely related to Paenibacillus yonginensis DCY84T and Paenibacillus physcomitrellae XBT (98.8 and 97.1 % sequence similarity).
29458522	9	2	theme	C15 	980:983	arg1	acid					954:957	The major fatty acid	938:957	The major fatty acid	938:957	The major fatty acid was anteiso-branched C15 : 0.
29458522	9	2	theme	C15 	980:983	arg1	 0					985:986	anteiso-branched C15 : 0	963:986	anteiso-branched C15 : 0	963:986	The major fatty acid was anteiso-branched C15 : 0.
29458522	12	3	theme	S8T	1134:1136	arg1	%					1212:1212	44 %	1209:1212	44 %	1209:1212	The DNA-DNA hybridization values of strain S8T with P. yonginensis KCTC 33428T and P. physcomitrellae DSM 29851T were 44 % and 32 %, respectively.
29458522	12	3	theme	S8T	1134:1136	arg1	values					1117:1122	The DNA-DNA hybridization values	1091:1122	The DNA-DNA hybridization values of strain S8T with P. yonginensis KCTC 33428T and P. physcomitrellae DSM 29851T	1091:1202	The DNA-DNA hybridization values of strain S8T with P. yonginensis KCTC 33428T and P. physcomitrellae DSM 29851T were 44 % and 32 %, respectively.
29458522	13	4	theme	=KCTC	1354:1358	arg1	31672T					1371:1376	=KCTC 33848T=JCM 31672T	1354:1376	=KCTC 33848T=JCM 31672T	1354:1376	Data from the DNA-DNA hybridization, biochemical, phylogenetic and physiological analyses indicate that strain S8T (=KCTC 33848T=JCM 31672T) represents a novel species of the genus Paenibacillus, for which the name Paenibacillus mobilis sp.
29458522	13	4	theme	=KCTC	1354:1358	arg1	S8T					1349:1351	strain S8T	1342:1351	strain S8T (=KCTC 33848T=JCM 31672T)	1342:1377	Data from the DNA-DNA hybridization, biochemical, phylogenetic and physiological analyses indicate that strain S8T (=KCTC 33848T=JCM 31672T) represents a novel species of the genus Paenibacillus, for which the name Paenibacillus mobilis sp.
29458522	7	5	theme	53.1±0.3 mol	767:778	arg1	content					735:741	The DNA G+C content	723:741	The DNA G+C content of the novel strain	723:761	The DNA G+C content of the novel strain was 53.1±0.3 mol%.
29458522	7	5	theme	53.1±0.3 mol	767:778	arg1	%					779:779	53.1±0.3 mol%	767:779	53.1±0.3 mol%	767:779	The DNA G+C content of the novel strain was 53.1±0.3 mol%.
29458522	12	6	theme	DNA-DNA	1095:1101	arg1	%					1212:1212	44 %	1209:1212	44 %	1209:1212	The DNA-DNA hybridization values of strain S8T with P. yonginensis KCTC 33428T and P. physcomitrellae DSM 29851T were 44 % and 32 %, respectively.
29458522	12	6	theme	DNA-DNA	1095:1101	arg1	values					1117:1122	The DNA-DNA hybridization values	1091:1122	The DNA-DNA hybridization values of strain S8T with P. yonginensis KCTC 33428T and P. physcomitrellae DSM 29851T	1091:1202	The DNA-DNA hybridization values of strain S8T with P. yonginensis KCTC 33428T and P. physcomitrellae DSM 29851T were 44 % and 32 %, respectively.
29458522	8	7	contain	contained	793:801	arg2	phosphatidylglycerol					827:846	phosphatidylglycerol	827:846	phosphatidylglycerol	827:846	Strain S8T contained diphosphatidylglycerol, phosphatidylglycerol, two phospholipids, four aminophospholipids, an aminolipid and three unidentified lipids.
29458522	8	7	contain	contained	793:801	arg2	phospholipids					853:865	two phospholipids	849:865	two phospholipids	849:865	Strain S8T contained diphosphatidylglycerol, phosphatidylglycerol, two phospholipids, four aminophospholipids, an aminolipid and three unidentified lipids.
29458522	8	7	contain	contained	793:801	arg2	aminolipid					896:905	an aminolipid	893:905	an aminolipid	893:905	Strain S8T contained diphosphatidylglycerol, phosphatidylglycerol, two phospholipids, four aminophospholipids, an aminolipid and three unidentified lipids.
29458522	8	7	contain	contained	793:801	arg2	diphosphatidylglycerol					803:824	diphosphatidylglycerol	803:824	diphosphatidylglycerol	803:824	Strain S8T contained diphosphatidylglycerol, phosphatidylglycerol, two phospholipids, four aminophospholipids, an aminolipid and three unidentified lipids.
29458522	8	7	contain	contained	793:801	arg2	lipids					930:935	three unidentified lipids	911:935	three unidentified lipids	911:935	Strain S8T contained diphosphatidylglycerol, phosphatidylglycerol, two phospholipids, four aminophospholipids, an aminolipid and three unidentified lipids.
29458522	8	7	contain	contained	793:801	arg2	aminophospholipids					873:890	four aminophospholipids	868:890	four aminophospholipids	868:890	Strain S8T contained diphosphatidylglycerol, phosphatidylglycerol, two phospholipids, four aminophospholipids, an aminolipid and three unidentified lipids.
29458522	8	7	contain	contained	793:801	arg1	S8T					789:791	Strain S8T	782:791	Strain S8T	782:791	Strain S8T contained diphosphatidylglycerol, phosphatidylglycerol, two phospholipids, four aminophospholipids, an aminolipid and three unidentified lipids.
29458522	13	8	theme	strain	1342:1347	arg1	31672T					1371:1376	=KCTC 33848T=JCM 31672T	1354:1376	=KCTC 33848T=JCM 31672T	1354:1376	Data from the DNA-DNA hybridization, biochemical, phylogenetic and physiological analyses indicate that strain S8T (=KCTC 33848T=JCM 31672T) represents a novel species of the genus Paenibacillus, for which the name Paenibacillus mobilis sp.
29458522	13	8	theme	strain	1342:1347	arg1	S8T					1349:1351	strain S8T	1342:1351	strain S8T (=KCTC 33848T=JCM 31672T)	1342:1377	Data from the DNA-DNA hybridization, biochemical, phylogenetic and physiological analyses indicate that strain S8T (=KCTC 33848T=JCM 31672T) represents a novel species of the genus Paenibacillus, for which the name Paenibacillus mobilis sp.
29458522	12	9	with	29851T	1197:1202	arg1	KCTC					1158:1161	P. yonginensis KCTC 33428T	1143:1168	P. yonginensis KCTC 33428T	1143:1168	The DNA-DNA hybridization values of strain S8T with P. yonginensis KCTC 33428T and P. physcomitrellae DSM 29851T were 44 % and 32 %, respectively.
29458522	9	10	theme	major	942:946	arg1	acid					954:957	The major fatty acid	938:957	The major fatty acid	938:957	The major fatty acid was anteiso-branched C15 : 0.
29458522	9	10	theme	major	942:946	arg1	 0					985:986	anteiso-branched C15 : 0	963:986	anteiso-branched C15 : 0	963:986	The major fatty acid was anteiso-branched C15 : 0.
29458522	6	11	theme	Paenibacillus	613:625	arg1	DCY84T					639:644	Paenibacillus yonginensis DCY84T	613:644	Paenibacillus yonginensis DCY84T	613:644	Based on 16S rRNA gene sequence similarity, strain S8T was affiliated with the genus Paenibacillus in the family Paenibacillaceae and was most closely related to Paenibacillus yonginensis DCY84T and Paenibacillus physcomitrellae XBT (98.8 and 97.1 % sequence similarity).
29458522	6	11	theme	Paenibacillus	613:625	arg1	similarity					710:719	98.8 and 97.1 % sequence similarity	685:719	98.8 and 97.1 % sequence similarity	685:719	Based on 16S rRNA gene sequence similarity, strain S8T was affiliated with the genus Paenibacillus in the family Paenibacillaceae and was most closely related to Paenibacillus yonginensis DCY84T and Paenibacillus physcomitrellae XBT (98.8 and 97.1 % sequence similarity).
29458522	8	12	theme	unidentified	917:928	arg1	lipids					930:935	three unidentified lipids	911:935	three unidentified lipids	911:935	Strain S8T contained diphosphatidylglycerol, phosphatidylglycerol, two phospholipids, four aminophospholipids, an aminolipid and three unidentified lipids.
29458522	8	12	theme	unidentified	917:928	arg1	diphosphatidylglycerol					803:824	diphosphatidylglycerol	803:824	diphosphatidylglycerol	803:824	Strain S8T contained diphosphatidylglycerol, phosphatidylglycerol, two phospholipids, four aminophospholipids, an aminolipid and three unidentified lipids.
29458522	7	13	theme	strain	756:761	arg1	content					735:741	The DNA G+C content	723:741	The DNA G+C content of the novel strain	723:761	The DNA G+C content of the novel strain was 53.1±0.3 mol%.
29458522	7	13	theme	strain	756:761	arg1	%					779:779	53.1±0.3 mol%	767:779	53.1±0.3 mol%	767:779	The DNA G+C content of the novel strain was 53.1±0.3 mol%.
29458522	13	14	theme	novel	1392:1396	arg1	species					1398:1404	a novel species	1390:1404	a novel species	1390:1404	Data from the DNA-DNA hybridization, biochemical, phylogenetic and physiological analyses indicate that strain S8T (=KCTC 33848T=JCM 31672T) represents a novel species of the genus Paenibacillus, for which the name Paenibacillus mobilis sp.
29458522	13	15	theme	33848T=JCM	1360:1369	arg1	31672T					1371:1376	=KCTC 33848T=JCM 31672T	1354:1376	=KCTC 33848T=JCM 31672T	1354:1376	Data from the DNA-DNA hybridization, biochemical, phylogenetic and physiological analyses indicate that strain S8T (=KCTC 33848T=JCM 31672T) represents a novel species of the genus Paenibacillus, for which the name Paenibacillus mobilis sp.
29458522	13	15	theme	33848T=JCM	1360:1369	arg1	S8T					1349:1351	strain S8T	1342:1351	strain S8T (=KCTC 33848T=JCM 31672T)	1342:1377	Data from the DNA-DNA hybridization, biochemical, phylogenetic and physiological analyses indicate that strain S8T (=KCTC 33848T=JCM 31672T) represents a novel species of the genus Paenibacillus, for which the name Paenibacillus mobilis sp.
29458522	12	16	theme	hybridization	1103:1115	arg1	%					1212:1212	44 %	1209:1212	44 %	1209:1212	The DNA-DNA hybridization values of strain S8T with P. yonginensis KCTC 33428T and P. physcomitrellae DSM 29851T were 44 % and 32 %, respectively.
29458522	12	16	theme	hybridization	1103:1115	arg1	values					1117:1122	The DNA-DNA hybridization values	1091:1122	The DNA-DNA hybridization values of strain S8T with P. yonginensis KCTC 33428T and P. physcomitrellae DSM 29851T	1091:1202	The DNA-DNA hybridization values of strain S8T with P. yonginensis KCTC 33428T and P. physcomitrellae DSM 29851T were 44 % and 32 %, respectively.
29458522	3	17	theme	strain	237:242	arg1	S8T					244:246	strain S8T	237:246	strain S8T	237:246	Cells of strain S8T were endospore-forming, motile by means of peritrichous flagella, and rod-shaped.
29458522	6	18	theme	sequence	701:708	arg1	DCY84T					639:644	Paenibacillus yonginensis DCY84T	613:644	Paenibacillus yonginensis DCY84T	613:644	Based on 16S rRNA gene sequence similarity, strain S8T was affiliated with the genus Paenibacillus in the family Paenibacillaceae and was most closely related to Paenibacillus yonginensis DCY84T and Paenibacillus physcomitrellae XBT (98.8 and 97.1 % sequence similarity).
29458522	6	18	theme	sequence	701:708	arg1	similarity					710:719	98.8 and 97.1 % sequence similarity	685:719	98.8 and 97.1 % sequence similarity	685:719	Based on 16S rRNA gene sequence similarity, strain S8T was affiliated with the genus Paenibacillus in the family Paenibacillaceae and was most closely related to Paenibacillus yonginensis DCY84T and Paenibacillus physcomitrellae XBT (98.8 and 97.1 % sequence similarity).
29458522	11	19	contain	contained	1055:1063	arg1	peptidoglycan					1027:1039	The peptidoglycan	1023:1039	The peptidoglycan of strain S8T	1023:1053	The peptidoglycan of strain S8T contained meso-diaminopimelic acid.
29458522	11	19	contain	contained	1055:1063	arg2	acid					1085:1088	meso-diaminopimelic acid	1065:1088	meso-diaminopimelic acid	1065:1088	The peptidoglycan of strain S8T contained meso-diaminopimelic acid.
29458522	8	20	theme	Strain	782:787	arg1	S8T					789:791	Strain S8T	782:791	Strain S8T	782:791	Strain S8T contained diphosphatidylglycerol, phosphatidylglycerol, two phospholipids, four aminophospholipids, an aminolipid and three unidentified lipids.
29458522	13	21	theme	genus	1413:1417	arg1	species					1398:1404	a novel species	1390:1404	a novel species	1390:1404	Data from the DNA-DNA hybridization, biochemical, phylogenetic and physiological analyses indicate that strain S8T (=KCTC 33848T=JCM 31672T) represents a novel species of the genus Paenibacillus, for which the name Paenibacillus mobilis sp.
29458522	0	22	theme	mobilis	14:20	arg1	sp					22:23	Paenibacillus mobilis sp	0:23	Paenibacillus mobilis sp.	0:24	Paenibacillus mobilis sp.
29458522	10	23	theme	menaquinone	1005:1015	arg1	quinone					993:999	The quinone	989:999	The quinone	989:999	The quinone was menaquinone MK-7.
29458522	10	23	theme	menaquinone	1005:1015	arg1	MK-7					1017:1020	menaquinone MK-7	1005:1020	menaquinone MK-7	1005:1020	The quinone was menaquinone MK-7.
29458522	6	24	theme	%	699:699	arg1	DCY84T					639:644	Paenibacillus yonginensis DCY84T	613:644	Paenibacillus yonginensis DCY84T	613:644	Based on 16S rRNA gene sequence similarity, strain S8T was affiliated with the genus Paenibacillus in the family Paenibacillaceae and was most closely related to Paenibacillus yonginensis DCY84T and Paenibacillus physcomitrellae XBT (98.8 and 97.1 % sequence similarity).
29458522	6	24	theme	%	699:699	arg1	similarity					710:719	98.8 and 97.1 % sequence similarity	685:719	98.8 and 97.1 % sequence similarity	685:719	Based on 16S rRNA gene sequence similarity, strain S8T was affiliated with the genus Paenibacillus in the family Paenibacillaceae and was most closely related to Paenibacillus yonginensis DCY84T and Paenibacillus physcomitrellae XBT (98.8 and 97.1 % sequence similarity).
29458522	7	25	theme	novel	750:754	arg1	strain					756:761	the novel strain	746:761	the novel strain	746:761	The DNA G+C content of the novel strain was 53.1±0.3 mol%.
29458522	13	26	theme	name	1448:1451	arg1	sp					1475:1476	the name Paenibacillus mobilis sp	1444:1476	the name Paenibacillus mobilis sp	1444:1476	Data from the DNA-DNA hybridization, biochemical, phylogenetic and physiological analyses indicate that strain S8T (=KCTC 33848T=JCM 31672T) represents a novel species of the genus Paenibacillus, for which the name Paenibacillus mobilis sp.
29458522	0	27	theme	Paenibacillus	0:12	arg1	sp					22:23	Paenibacillus mobilis sp	0:23	Paenibacillus mobilis sp.	0:24	Paenibacillus mobilis sp.
29458522	13	28	theme	physiological	1305:1317	arg1	analyses					1319:1326	biochemical, phylogenetic and physiological analyses	1275:1326	biochemical, phylogenetic and physiological analyses	1275:1326	Data from the DNA-DNA hybridization, biochemical, phylogenetic and physiological analyses indicate that strain S8T (=KCTC 33848T=JCM 31672T) represents a novel species of the genus Paenibacillus, for which the name Paenibacillus mobilis sp.
29458522	6	29	from	Paenibacillaceae	564:579	arg1	Paenibacillus					536:548	Paenibacillus	536:548	Paenibacillus	536:548	Based on 16S rRNA gene sequence similarity, strain S8T was affiliated with the genus Paenibacillus in the family Paenibacillaceae and was most closely related to Paenibacillus yonginensis DCY84T and Paenibacillus physcomitrellae XBT (98.8 and 97.1 % sequence similarity).
29458522	13	30	theme	biochemical	1275:1285	arg1	analyses					1319:1326	biochemical, phylogenetic and physiological analyses	1275:1326	biochemical, phylogenetic and physiological analyses	1275:1326	Data from the DNA-DNA hybridization, biochemical, phylogenetic and physiological analyses indicate that strain S8T (=KCTC 33848T=JCM 31672T) represents a novel species of the genus Paenibacillus, for which the name Paenibacillus mobilis sp.
29458522	12	31	with	S8T	1134:1136	arg1	KCTC					1158:1161	P. yonginensis KCTC 33428T	1143:1168	P. yonginensis KCTC 33428T	1143:1168	The DNA-DNA hybridization values of strain S8T with P. yonginensis KCTC 33428T and P. physcomitrellae DSM 29851T were 44 % and 32 %, respectively.
29458522	6	32	theme	strain	495:500	arg1	S8T					502:504	strain S8T	495:504	strain S8T	495:504	Based on 16S rRNA gene sequence similarity, strain S8T was affiliated with the genus Paenibacillus in the family Paenibacillaceae and was most closely related to Paenibacillus yonginensis DCY84T and Paenibacillus physcomitrellae XBT (98.8 and 97.1 % sequence similarity).
29458522	13	33	theme	Paenibacillus	1419:1431	arg1	genus					1413:1417	the genus Paenibacillus	1409:1431	the genus Paenibacillus	1409:1431	Data from the DNA-DNA hybridization, biochemical, phylogenetic and physiological analyses indicate that strain S8T (=KCTC 33848T=JCM 31672T) represents a novel species of the genus Paenibacillus, for which the name Paenibacillus mobilis sp.
29458522	12	34	dep	P.	1174:1175	arg1	physcomitrellae					1177:1191	physcomitrellae	1177:1191	physcomitrellae	1177:1191	The DNA-DNA hybridization values of strain S8T with P. yonginensis KCTC 33428T and P. physcomitrellae DSM 29851T were 44 % and 32 %, respectively.
29458522	5	35	dep	NaCl	445:448	arg1	%					437:437	%	437:437	%	437:437	Strain S8T grew optimally at 37 °C, pH 6-8, and up to 2.0 % (w/v) NaCl.
29458522	7	36	theme	DNA	727:729	arg1	content					735:741	The DNA G+C content	723:741	The DNA G+C content of the novel strain	723:761	The DNA G+C content of the novel strain was 53.1±0.3 mol%.
29458522	7	36	theme	DNA	727:729	arg1	%					779:779	53.1±0.3 mol%	767:779	53.1±0.3 mol%	767:779	The DNA G+C content of the novel strain was 53.1±0.3 mol%.
29458522	12	37	theme	29851T	1197:1202	arg1	%					1212:1212	44 %	1209:1212	44 %	1209:1212	The DNA-DNA hybridization values of strain S8T with P. yonginensis KCTC 33428T and P. physcomitrellae DSM 29851T were 44 % and 32 %, respectively.
29458522	12	37	theme	29851T	1197:1202	arg1	values					1117:1122	The DNA-DNA hybridization values	1091:1122	The DNA-DNA hybridization values of strain S8T with P. yonginensis KCTC 33428T and P. physcomitrellae DSM 29851T	1091:1202	The DNA-DNA hybridization values of strain S8T with P. yonginensis KCTC 33428T and P. physcomitrellae DSM 29851T were 44 % and 32 %, respectively.
29458522	3	38	theme	S8T	244:246	arg1	Cells					228:232	Cells	228:232	Cells of strain S8T	228:246	Cells of strain S8T were endospore-forming, motile by means of peritrichous flagella, and rod-shaped.
29458522	2	39	theme	soil	166:169	arg1	sample					171:176	a soil sample	164:176	a soil sample obtained in Gyeonggi Province, Republic of Korea	164:225	A novel Gram-stain-negative bacterium, designated strain S8T, was isolated from a soil sample obtained in Gyeonggi Province, Republic of Korea.
29458522	11	40	theme	strain	1044:1049	arg1	S8T					1051:1053	strain S8T	1044:1053	strain S8T	1044:1053	The peptidoglycan of strain S8T contained meso-diaminopimelic acid.
29458522	7	41	theme	G+C	731:733	arg1	content					735:741	The DNA G+C content	723:741	The DNA G+C content of the novel strain	723:761	The DNA G+C content of the novel strain was 53.1±0.3 mol%.
29458522	7	41	theme	G+C	731:733	arg1	%					779:779	53.1±0.3 mol%	767:779	53.1±0.3 mol%	767:779	The DNA G+C content of the novel strain was 53.1±0.3 mol%.
29458522	13	42	theme	Paenibacillus	1453:1465	arg1	sp					1475:1476	the name Paenibacillus mobilis sp	1444:1476	the name Paenibacillus mobilis sp	1444:1476	Data from the DNA-DNA hybridization, biochemical, phylogenetic and physiological analyses indicate that strain S8T (=KCTC 33848T=JCM 31672T) represents a novel species of the genus Paenibacillus, for which the name Paenibacillus mobilis sp.
29458522	6	43	theme	sequence	474:481	arg1	similarity					483:492	16S rRNA gene sequence similarity	460:492	16S rRNA gene sequence similarity	460:492	Based on 16S rRNA gene sequence similarity, strain S8T was affiliated with the genus Paenibacillus in the family Paenibacillaceae and was most closely related to Paenibacillus yonginensis DCY84T and Paenibacillus physcomitrellae XBT (98.8 and 97.1 % sequence similarity).
29458522	2	44	theme	Gram-stain-negative	92:110	arg1	bacterium					112:120	A novel Gram-stain-negative bacterium	84:120	A novel Gram-stain-negative bacterium	84:120	A novel Gram-stain-negative bacterium, designated strain S8T, was isolated from a soil sample obtained in Gyeonggi Province, Republic of Korea.
29458522	6	45	from	Paenibacillus	536:548	arg1	Paenibacillaceae					564:579	the family Paenibacillaceae	553:579	the family Paenibacillaceae	553:579	Based on 16S rRNA gene sequence similarity, strain S8T was affiliated with the genus Paenibacillus in the family Paenibacillaceae and was most closely related to Paenibacillus yonginensis DCY84T and Paenibacillus physcomitrellae XBT (98.8 and 97.1 % sequence similarity).
29458522	5	46	dep	2.0 	433:436	arg1	to					430:431	to	430:431	to	430:431	Strain S8T grew optimally at 37 °C, pH 6-8, and up to 2.0 % (w/v) NaCl.
29458522	6	47	theme	gene	469:472	arg1	similarity					483:492	16S rRNA gene sequence similarity	460:492	16S rRNA gene sequence similarity	460:492	Based on 16S rRNA gene sequence similarity, strain S8T was affiliated with the genus Paenibacillus in the family Paenibacillaceae and was most closely related to Paenibacillus yonginensis DCY84T and Paenibacillus physcomitrellae XBT (98.8 and 97.1 % sequence similarity).
29458522	2	48	theme	novel	86:90	arg1	bacterium					112:120	A novel Gram-stain-negative bacterium	84:120	A novel Gram-stain-negative bacterium	84:120	A novel Gram-stain-negative bacterium, designated strain S8T, was isolated from a soil sample obtained in Gyeonggi Province, Republic of Korea.
29458522	13	49	from	hybridization	1260:1272	arg1	Data					1238:1241	Data	1238:1241	Data from the DNA-DNA hybridization	1238:1272	Data from the DNA-DNA hybridization, biochemical, phylogenetic and physiological analyses indicate that strain S8T (=KCTC 33848T=JCM 31672T) represents a novel species of the genus Paenibacillus, for which the name Paenibacillus mobilis sp.
29458522	5	50	dep	%	437:437	arg1	2.0 					433:436	2.0 	433:436	2.0 	433:436	Strain S8T grew optimally at 37 °C, pH 6-8, and up to 2.0 % (w/v) NaCl.
29458522	5	50	dep	%	437:437	arg1	w/v					440:442	w/v	440:442	w/v	440:442	Strain S8T grew optimally at 37 °C, pH 6-8, and up to 2.0 % (w/v) NaCl.
29458522	11	51	theme	S8T	1051:1053	arg1	peptidoglycan					1027:1039	The peptidoglycan	1023:1039	The peptidoglycan of strain S8T	1023:1053	The peptidoglycan of strain S8T contained meso-diaminopimelic acid.
29458522	6	52	theme	rRNA	464:467	arg1	similarity					483:492	16S rRNA gene sequence similarity	460:492	16S rRNA gene sequence similarity	460:492	Based on 16S rRNA gene sequence similarity, strain S8T was affiliated with the genus Paenibacillus in the family Paenibacillaceae and was most closely related to Paenibacillus yonginensis DCY84T and Paenibacillus physcomitrellae XBT (98.8 and 97.1 % sequence similarity).
29458522	13	53	theme	phylogenetic	1288:1299	arg1	analyses					1319:1326	biochemical, phylogenetic and physiological analyses	1275:1326	biochemical, phylogenetic and physiological analyses	1275:1326	Data from the DNA-DNA hybridization, biochemical, phylogenetic and physiological analyses indicate that strain S8T (=KCTC 33848T=JCM 31672T) represents a novel species of the genus Paenibacillus, for which the name Paenibacillus mobilis sp.
29458522	12	54	theme	strain	1127:1132	arg1	S8T					1134:1136	strain S8T	1127:1136	strain S8T with P. yonginensis KCTC 33428T	1127:1168	The DNA-DNA hybridization values of strain S8T with P. yonginensis KCTC 33428T and P. physcomitrellae DSM 29851T were 44 % and 32 %, respectively.
29458522	12	55	theme	P.	1174:1175	arg1	29851T					1197:1202	P. physcomitrellae DSM 29851T	1174:1202	P. physcomitrellae DSM 29851T	1174:1202	The DNA-DNA hybridization values of strain S8T with P. yonginensis KCTC 33428T and P. physcomitrellae DSM 29851T were 44 % and 32 %, respectively.
29458522	2	56	attach	isolated	150:157	arg2	bacterium					112:120	A novel Gram-stain-negative bacterium	84:120	A novel Gram-stain-negative bacterium	84:120	A novel Gram-stain-negative bacterium, designated strain S8T, was isolated from a soil sample obtained in Gyeonggi Province, Republic of Korea.
29458522	2	56	attach	isolated	150:157	arg1	sample					171:176	a soil sample	164:176	a soil sample obtained in Gyeonggi Province, Republic of Korea	164:225	A novel Gram-stain-negative bacterium, designated strain S8T, was isolated from a soil sample obtained in Gyeonggi Province, Republic of Korea.
29458522	6	57	theme	family	557:562	arg1	Paenibacillaceae					564:579	the family Paenibacillaceae	553:579	the family Paenibacillaceae	553:579	Based on 16S rRNA gene sequence similarity, strain S8T was affiliated with the genus Paenibacillus in the family Paenibacillaceae and was most closely related to Paenibacillus yonginensis DCY84T and Paenibacillus physcomitrellae XBT (98.8 and 97.1 % sequence similarity).
29458522	11	58	theme	meso-diaminopimelic	1065:1083	arg1	acid					1085:1088	meso-diaminopimelic acid	1065:1088	meso-diaminopimelic acid	1065:1088	The peptidoglycan of strain S8T contained meso-diaminopimelic acid.
29458522	13	59	theme	mobilis	1467:1473	arg1	sp					1475:1476	the name Paenibacillus mobilis sp	1444:1476	the name Paenibacillus mobilis sp	1444:1476	Data from the DNA-DNA hybridization, biochemical, phylogenetic and physiological analyses indicate that strain S8T (=KCTC 33848T=JCM 31672T) represents a novel species of the genus Paenibacillus, for which the name Paenibacillus mobilis sp.
29458522	4	60	theme	S8T	330:332	arg1	colonies					334:341	S8T colonies	330:341	S8T colonies	330:341	S8T colonies were round, convex, wavy and white.
29458522	12	61	theme	DSM	1193:1195	arg1	29851T					1197:1202	P. physcomitrellae DSM 29851T	1174:1202	P. physcomitrellae DSM 29851T	1174:1202	The DNA-DNA hybridization values of strain S8T with P. yonginensis KCTC 33428T and P. physcomitrellae DSM 29851T were 44 % and 32 %, respectively.
29458522	9	62	theme	fatty	948:952	arg1	acid					954:957	The major fatty acid	938:957	The major fatty acid	938:957	The major fatty acid was anteiso-branched C15 : 0.
29458522	9	62	theme	fatty	948:952	arg1	 0					985:986	anteiso-branched C15 : 0	963:986	anteiso-branched C15 : 0	963:986	The major fatty acid was anteiso-branched C15 : 0.
29458522	6	63	theme	physcomitrellae	664:678	arg1	XBT					680:682	Paenibacillus physcomitrellae XBT	650:682	Paenibacillus physcomitrellae XBT	650:682	Based on 16S rRNA gene sequence similarity, strain S8T was affiliated with the genus Paenibacillus in the family Paenibacillaceae and was most closely related to Paenibacillus yonginensis DCY84T and Paenibacillus physcomitrellae XBT (98.8 and 97.1 % sequence similarity).
29458522	6	64	theme	Paenibacillus	650:662	arg1	XBT					680:682	Paenibacillus physcomitrellae XBT	650:682	Paenibacillus physcomitrellae XBT	650:682	Based on 16S rRNA gene sequence similarity, strain S8T was affiliated with the genus Paenibacillus in the family Paenibacillaceae and was most closely related to Paenibacillus yonginensis DCY84T and Paenibacillus physcomitrellae XBT (98.8 and 97.1 % sequence similarity).
29458522	13	65	theme	DNA-DNA	1252:1258	arg1	hybridization					1260:1272	the DNA-DNA hybridization	1248:1272	the DNA-DNA hybridization	1248:1272	Data from the DNA-DNA hybridization, biochemical, phylogenetic and physiological analyses indicate that strain S8T (=KCTC 33848T=JCM 31672T) represents a novel species of the genus Paenibacillus, for which the name Paenibacillus mobilis sp.
29458522	6	66	theme	16S	460:462	arg1	similarity					483:492	16S rRNA gene sequence similarity	460:492	16S rRNA gene sequence similarity	460:492	Based on 16S rRNA gene sequence similarity, strain S8T was affiliated with the genus Paenibacillus in the family Paenibacillaceae and was most closely related to Paenibacillus yonginensis DCY84T and Paenibacillus physcomitrellae XBT (98.8 and 97.1 % sequence similarity).
29458522	12	67	theme	yonginensis	1146:1156	arg1	KCTC					1158:1161	P. yonginensis KCTC 33428T	1143:1168	P. yonginensis KCTC 33428T	1143:1168	The DNA-DNA hybridization values of strain S8T with P. yonginensis KCTC 33428T and P. physcomitrellae DSM 29851T were 44 % and 32 %, respectively.
29458522	2	68	theme	strain	134:139	arg1	S8T					141:143	strain S8T	134:143	strain S8T	134:143	A novel Gram-stain-negative bacterium, designated strain S8T, was isolated from a soil sample obtained in Gyeonggi Province, Republic of Korea.
29458522	1	69	theme	Gram-stain-negative	34:52	arg1	bacterium					54:62	a Gram-stain-negative bacterium	32:62	a Gram-stain-negative bacterium	32:62	nov., a Gram-stain-negative bacterium isolated from soil.
29458522	1	69	theme	Gram-stain-negative	34:52	arg1	nov.					26:29	nov.	26:29	nov.	26:29	nov., a Gram-stain-negative bacterium isolated from soil.
29458522	12	70	theme	P.	1143:1144	arg1	KCTC					1158:1161	P. yonginensis KCTC 33428T	1143:1168	P. yonginensis KCTC 33428T	1143:1168	The DNA-DNA hybridization values of strain S8T with P. yonginensis KCTC 33428T and P. physcomitrellae DSM 29851T were 44 % and 32 %, respectively.
29458522	9	71	theme	anteiso-branched	963:978	arg1	acid					954:957	The major fatty acid	938:957	The major fatty acid	938:957	The major fatty acid was anteiso-branched C15 : 0.
29458522	9	71	theme	anteiso-branched	963:978	arg1	 0					985:986	anteiso-branched C15 : 0	963:986	anteiso-branched C15 : 0	963:986	The major fatty acid was anteiso-branched C15 : 0.
30361979	4	0	from	pH	526:527	arg1	at					523:524	optimal at pH 9.0	515:531	optimal at pH 9.0	515:531	Cell growth was observed in a medium containing 0-25% (w/v) NaCl (optimal at 10% [w/v]), at 20-40°C (optimal at 37°C) and pH 6.5-10.0 (optimal at pH 9.0).
30361979	8	1	theme	rRNA	929:932	arg1	similarity					948:957	92.7-92.9% 16S rRNA gene sequence similarity	914:957	92.7-92.9% 16S rRNA gene sequence similarity	914:957	Phylogenetic analysis indicated the strain to be most closely related to Geomicrobium halophilum with 92.7-92.9% 16S rRNA gene sequence similarity.
30361979	9	2	theme	gen.	1157:1160	arg1	nov.					1162:1165	the name Salicibibacter kimchii gen. nov.	1125:1165	the name Salicibibacter kimchii gen. nov.	1125:1165	Based on polyphasic taxonomic evaluation with phenotypic, phylogenetic, and chemotaxonomic analyses, the strain represents a novel species in a new genus, for which the name Salicibibacter kimchii gen. nov., sp.
30361979	6	3	theme	major	756:760	arg1	acids					768:772	the major fatty acids	752:772	the major fatty acids	752:772	Cell membrane of the strain contained iso-C17:0 and anteiso-C15:0 as the major fatty acids.
30361979	6	3	theme	major	756:760	arg1	anteiso-C15:0					735:747	anteiso-C15:0	735:747	anteiso-C15:0	735:747	Cell membrane of the strain contained iso-C17:0 and anteiso-C15:0 as the major fatty acids.
30361979	6	3	theme	major	756:760	arg1	iso-C17:0					721:729	iso-C17:0	721:729	iso-C17:0	721:729	Cell membrane of the strain contained iso-C17:0 and anteiso-C15:0 as the major fatty acids.
30361979	9	4	theme	polyphasic	969:978	arg1	evaluation					990:999	polyphasic taxonomic evaluation	969:999	polyphasic taxonomic evaluation with phenotypic, phylogenetic, and chemotaxonomic analyses	969:1058	Based on polyphasic taxonomic evaluation with phenotypic, phylogenetic, and chemotaxonomic analyses, the strain represents a novel species in a new genus, for which the name Salicibibacter kimchii gen. nov., sp.
30361979	5	5	theme	isolate	571:577	arg1	menaquinone-7					583:595	menaquinone-7	583:595	menaquinone-7	583:595	The major isoprenoid quinone of the isolate was menaquinone-7, and the major polar lipids were phosphatidylglycerol and unidentified phospholipids.
30361979	5	5	theme	isolate	571:577	arg1	quinone					556:562	The major isoprenoid quinone	535:562	The major isoprenoid quinone of the isolate	535:577	The major isoprenoid quinone of the isolate was menaquinone-7, and the major polar lipids were phosphatidylglycerol and unidentified phospholipids.
30361979	4	6	dep	NaCl	440:443	arg1	optimal					446:452	optimal	446:452	optimal	446:452	Cell growth was observed in a medium containing 0-25% (w/v) NaCl (optimal at 10% [w/v]), at 20-40°C (optimal at 37°C) and pH 6.5-10.0 (optimal at pH 9.0).
30361979	3	7	theme	acid-type	362:370	arg1	murein					372:377	diaminopimelic acid-type murein	347:377	diaminopimelic acid-type murein	347:377	Strain NKC1-1T was Gram-stain-positive, aerobic, rod-shaped, non-motile, and contained diaminopimelic acid-type murein.
30361979	8	8	theme	sequence	939:946	arg1	similarity					948:957	92.7-92.9% 16S rRNA gene sequence similarity	914:957	92.7-92.9% 16S rRNA gene sequence similarity	914:957	Phylogenetic analysis indicated the strain to be most closely related to Geomicrobium halophilum with 92.7-92.9% 16S rRNA gene sequence similarity.
30361979	7	9	theme	C	787:787	arg1	content					789:795	Its DNA G + C content	775:795	content	789:795	Its DNA G + C content was 45.2 mol%.
30361979	4	10	theme	optimal	515:521	arg1	pH					526:527	optimal at pH 9.0	515:531	optimal at pH 9.0	515:531	Cell growth was observed in a medium containing 0-25% (w/v) NaCl (optimal at 10% [w/v]), at 20-40°C (optimal at 37°C) and pH 6.5-10.0 (optimal at pH 9.0).
30361979	4	10	theme	optimal	515:521	arg1	pH					502:503	pH 6.5-10.0	502:512	pH 6.5-10.0 (optimal at pH 9.0)	502:532	Cell growth was observed in a medium containing 0-25% (w/v) NaCl (optimal at 10% [w/v]), at 20-40°C (optimal at 37°C) and pH 6.5-10.0 (optimal at pH 9.0).
30361979	5	11	theme	phosphatidylglycerol	630:649	arg1	phospholipids					668:680	phosphatidylglycerol and unidentified phospholipids	630:680	phosphatidylglycerol and unidentified phospholipids	630:680	The major isoprenoid quinone of the isolate was menaquinone-7, and the major polar lipids were phosphatidylglycerol and unidentified phospholipids.
30361979	5	11	theme	phosphatidylglycerol	630:649	arg1	lipids					618:623	the major polar lipids	602:623	the major polar lipids	602:623	The major isoprenoid quinone of the isolate was menaquinone-7, and the major polar lipids were phosphatidylglycerol and unidentified phospholipids.
30361979	4	12	from	[w/v	461:464	arg1	optimal					446:452	optimal	446:452	optimal	446:452	Cell growth was observed in a medium containing 0-25% (w/v) NaCl (optimal at 10% [w/v]), at 20-40°C (optimal at 37°C) and pH 6.5-10.0 (optimal at pH 9.0).
30361979	7	13	theme	DNA	779:781	arg1	G					783:783	Its DNA G + C content	775:795	G	783:783	Its DNA G + C content was 45.2 mol%.
30361979	2	14	attach	isolated	218:225	arg2	NKC1-1T					206:212	A moderately halophilic and alkalitolerant bacterial strain NKC1-1T	146:212	A moderately halophilic and alkalitolerant bacterial strain NKC1-1T	146:212	A moderately halophilic and alkalitolerant bacterial strain NKC1-1T was isolated from commercial kimchi in Korea.
30361979	2	14	attach	isolated	218:225	arg1	kimchi					243:248	commercial kimchi	232:248	commercial kimchi in Korea	232:257	A moderately halophilic and alkalitolerant bacterial strain NKC1-1T was isolated from commercial kimchi in Korea.
30361979	6	15	contain	contained	711:719	arg2	anteiso-C15:0					735:747	anteiso-C15:0	735:747	anteiso-C15:0	735:747	Cell membrane of the strain contained iso-C17:0 and anteiso-C15:0 as the major fatty acids.
30361979	6	15	contain	contained	711:719	arg2	acids					768:772	the major fatty acids	752:772	the major fatty acids	752:772	Cell membrane of the strain contained iso-C17:0 and anteiso-C15:0 as the major fatty acids.
30361979	6	15	contain	contained	711:719	arg2	iso-C17:0					721:729	iso-C17:0	721:729	iso-C17:0	721:729	Cell membrane of the strain contained iso-C17:0 and anteiso-C15:0 as the major fatty acids.
30361979	6	15	contain	contained	711:719	arg1	membrane					688:695	Cell membrane	683:695	Cell membrane of the strain	683:709	Cell membrane of the strain contained iso-C17:0 and anteiso-C15:0 as the major fatty acids.
30361979	10	16	dep	proposed	1180:1187	arg1	43276T					1209:1214	= CECT 9537T; KCCM 43276T	1190:1214	= CECT 9537T; KCCM 43276T	1190:1214	nov. is proposed (= CECT 9537T; KCCM 43276T).
30361979	9	17	theme	taxonomic	980:988	arg1	evaluation					990:999	polyphasic taxonomic evaluation	969:999	polyphasic taxonomic evaluation with phenotypic, phylogenetic, and chemotaxonomic analyses	969:1058	Based on polyphasic taxonomic evaluation with phenotypic, phylogenetic, and chemotaxonomic analyses, the strain represents a novel species in a new genus, for which the name Salicibibacter kimchii gen. nov., sp.
30361979	8	18	theme	gene	934:937	arg1	similarity					948:957	92.7-92.9% 16S rRNA gene sequence similarity	914:957	92.7-92.9% 16S rRNA gene sequence similarity	914:957	Phylogenetic analysis indicated the strain to be most closely related to Geomicrobium halophilum with 92.7-92.9% 16S rRNA gene sequence similarity.
30361979	3	19	theme	diaminopimelic	347:360	arg1	murein					372:377	diaminopimelic acid-type murein	347:377	diaminopimelic acid-type murein	347:377	Strain NKC1-1T was Gram-stain-positive, aerobic, rod-shaped, non-motile, and contained diaminopimelic acid-type murein.
30361979	4	20	theme	%	459:459	arg1	[w/v					461:464	10% [w/v	457:464	10% [w/v	457:464	Cell growth was observed in a medium containing 0-25% (w/v) NaCl (optimal at 10% [w/v]), at 20-40°C (optimal at 37°C) and pH 6.5-10.0 (optimal at pH 9.0).
30361979	4	21	theme	Cell	380:383	arg1	growth					385:390	Cell growth	380:390	Cell growth	380:390	Cell growth was observed in a medium containing 0-25% (w/v) NaCl (optimal at 10% [w/v]), at 20-40°C (optimal at 37°C) and pH 6.5-10.0 (optimal at pH 9.0).
30361979	1	22	theme	halophilic	57:66	arg1	bacterium					87:95	a moderately halophilic and alkalitolerant bacterium	44:95	a moderately halophilic and alkalitolerant bacterium in the family Bacillaceae	44:121	nov., a moderately halophilic and alkalitolerant bacterium in the family Bacillaceae, isolated from kimchi.
30361979	1	22	theme	halophilic	57:66	arg1	nov.					38:41	nov.	38:41	nov.	38:41	nov., a moderately halophilic and alkalitolerant bacterium in the family Bacillaceae, isolated from kimchi.
30361979	0	23	theme	kimchii	15:21	arg1	nov.					28:31	Salicibibacter kimchii gen. nov.	0:31	Salicibibacter kimchii gen. nov.	0:31	Salicibibacter kimchii gen. nov., sp.
30361979	4	24	theme	10	457:458	arg1	%					459:459	%	459:459	%	459:459	Cell growth was observed in a medium containing 0-25% (w/v) NaCl (optimal at 10% [w/v]), at 20-40°C (optimal at 37°C) and pH 6.5-10.0 (optimal at pH 9.0).
30361979	5	25	theme	major	539:543	arg1	menaquinone-7					583:595	menaquinone-7	583:595	menaquinone-7	583:595	The major isoprenoid quinone of the isolate was menaquinone-7, and the major polar lipids were phosphatidylglycerol and unidentified phospholipids.
30361979	5	25	theme	major	539:543	arg1	quinone					556:562	The major isoprenoid quinone	535:562	The major isoprenoid quinone of the isolate	535:577	The major isoprenoid quinone of the isolate was menaquinone-7, and the major polar lipids were phosphatidylglycerol and unidentified phospholipids.
30361979	6	26	theme	strain	704:709	arg1	membrane					688:695	Cell membrane	683:695	Cell membrane of the strain	683:709	Cell membrane of the strain contained iso-C17:0 and anteiso-C15:0 as the major fatty acids.
30361979	4	27	contain	containing	417:426	arg2	NaCl					440:443	0-25% (w/v) NaCl	428:443	0-25% (w/v) NaCl (optimal at 10% [w/v])	428:466	Cell growth was observed in a medium containing 0-25% (w/v) NaCl (optimal at 10% [w/v]), at 20-40°C (optimal at 37°C) and pH 6.5-10.0 (optimal at pH 9.0).
30361979	4	27	contain	containing	417:426	arg1	medium					410:415	a medium	408:415	a medium containing 0-25% (w/v) NaCl (optimal at 10% [w/v])	408:466	Cell growth was observed in a medium containing 0-25% (w/v) NaCl (optimal at 10% [w/v]), at 20-40°C (optimal at 37°C) and pH 6.5-10.0 (optimal at pH 9.0).
30361979	0	28	theme	Salicibibacter	0:13	arg1	nov.					28:31	Salicibibacter kimchii gen. nov.	0:31	Salicibibacter kimchii gen. nov.	0:31	Salicibibacter kimchii gen. nov., sp.
30361979	5	29	theme	polar	612:616	arg1	phospholipids					668:680	phosphatidylglycerol and unidentified phospholipids	630:680	phosphatidylglycerol and unidentified phospholipids	630:680	The major isoprenoid quinone of the isolate was menaquinone-7, and the major polar lipids were phosphatidylglycerol and unidentified phospholipids.
30361979	5	29	theme	polar	612:616	arg1	lipids					618:623	the major polar lipids	602:623	the major polar lipids	602:623	The major isoprenoid quinone of the isolate was menaquinone-7, and the major polar lipids were phosphatidylglycerol and unidentified phospholipids.
30361979	2	30	theme	alkalitolerant	174:187	arg1	NKC1-1T					206:212	A moderately halophilic and alkalitolerant bacterial strain NKC1-1T	146:212	A moderately halophilic and alkalitolerant bacterial strain NKC1-1T	146:212	A moderately halophilic and alkalitolerant bacterial strain NKC1-1T was isolated from commercial kimchi in Korea.
30361979	8	31	theme	%	923:923	arg1	similarity					948:957	92.7-92.9% 16S rRNA gene sequence similarity	914:957	92.7-92.9% 16S rRNA gene sequence similarity	914:957	Phylogenetic analysis indicated the strain to be most closely related to Geomicrobium halophilum with 92.7-92.9% 16S rRNA gene sequence similarity.
30361979	1	32	theme	alkalitolerant	72:85	arg1	bacterium					87:95	a moderately halophilic and alkalitolerant bacterium	44:95	a moderately halophilic and alkalitolerant bacterium in the family Bacillaceae	44:121	nov., a moderately halophilic and alkalitolerant bacterium in the family Bacillaceae, isolated from kimchi.
30361979	1	32	theme	alkalitolerant	72:85	arg1	nov.					38:41	nov.	38:41	nov.	38:41	nov., a moderately halophilic and alkalitolerant bacterium in the family Bacillaceae, isolated from kimchi.
30361979	9	33	theme	phenotypic	1006:1015	arg1	analyses					1051:1058	phenotypic, phylogenetic, and chemotaxonomic analyses	1006:1058	phenotypic, phylogenetic, and chemotaxonomic analyses	1006:1058	Based on polyphasic taxonomic evaluation with phenotypic, phylogenetic, and chemotaxonomic analyses, the strain represents a novel species in a new genus, for which the name Salicibibacter kimchii gen. nov., sp.
30361979	4	34	theme	w/v	435:437	arg1	NaCl					440:443	0-25% (w/v) NaCl	428:443	0-25% (w/v) NaCl (optimal at 10% [w/v])	428:466	Cell growth was observed in a medium containing 0-25% (w/v) NaCl (optimal at 10% [w/v]), at 20-40°C (optimal at 37°C) and pH 6.5-10.0 (optimal at pH 9.0).
30361979	4	35	from	37°C	492:495	arg1	at					489:490	optimal at 37°C	481:495	optimal at 37°C	481:495	Cell growth was observed in a medium containing 0-25% (w/v) NaCl (optimal at 10% [w/v]), at 20-40°C (optimal at 37°C) and pH 6.5-10.0 (optimal at pH 9.0).
30361979	4	36	theme	at	489:490	arg1	37°C					492:495	optimal at 37°C	481:495	optimal at 37°C	481:495	Cell growth was observed in a medium containing 0-25% (w/v) NaCl (optimal at 10% [w/v]), at 20-40°C (optimal at 37°C) and pH 6.5-10.0 (optimal at pH 9.0).
30361979	4	36	theme	at	489:490	arg1	20-40°C					472:478	20-40°C	472:478	20-40°C (optimal at 37°C)	472:496	Cell growth was observed in a medium containing 0-25% (w/v) NaCl (optimal at 10% [w/v]), at 20-40°C (optimal at 37°C) and pH 6.5-10.0 (optimal at pH 9.0).
30361979	8	37	theme	92.7-92.9	914:922	arg1	%					923:923	%	923:923	%	923:923	Phylogenetic analysis indicated the strain to be most closely related to Geomicrobium halophilum with 92.7-92.9% 16S rRNA gene sequence similarity.
30361979	9	38	with	evaluation	990:999	arg1	analyses					1051:1058	phenotypic, phylogenetic, and chemotaxonomic analyses	1006:1058	phenotypic, phylogenetic, and chemotaxonomic analyses	1006:1058	Based on polyphasic taxonomic evaluation with phenotypic, phylogenetic, and chemotaxonomic analyses, the strain represents a novel species in a new genus, for which the name Salicibibacter kimchii gen. nov., sp.
30361979	2	39	from	kimchi	243:248	arg1	Korea					253:257	Korea	253:257	Korea	253:257	A moderately halophilic and alkalitolerant bacterial strain NKC1-1T was isolated from commercial kimchi in Korea.
30361979	0	40	theme	gen.	23:26	arg1	nov.					28:31	Salicibibacter kimchii gen. nov.	0:31	Salicibibacter kimchii gen. nov.	0:31	Salicibibacter kimchii gen. nov., sp.
30361979	9	41	dep	sp	1168:1169	arg1	nov.					1162:1165	the name Salicibibacter kimchii gen. nov.	1125:1165	the name Salicibibacter kimchii gen. nov.	1125:1165	Based on polyphasic taxonomic evaluation with phenotypic, phylogenetic, and chemotaxonomic analyses, the strain represents a novel species in a new genus, for which the name Salicibibacter kimchii gen. nov., sp.
30361979	5	42	theme	major	606:610	arg1	phospholipids					668:680	phosphatidylglycerol and unidentified phospholipids	630:680	phosphatidylglycerol and unidentified phospholipids	630:680	The major isoprenoid quinone of the isolate was menaquinone-7, and the major polar lipids were phosphatidylglycerol and unidentified phospholipids.
30361979	5	42	theme	major	606:610	arg1	lipids					618:623	the major polar lipids	602:623	the major polar lipids	602:623	The major isoprenoid quinone of the isolate was menaquinone-7, and the major polar lipids were phosphatidylglycerol and unidentified phospholipids.
30361979	9	43	theme	phylogenetic	1018:1029	arg1	analyses					1051:1058	phenotypic, phylogenetic, and chemotaxonomic analyses	1006:1058	phenotypic, phylogenetic, and chemotaxonomic analyses	1006:1058	Based on polyphasic taxonomic evaluation with phenotypic, phylogenetic, and chemotaxonomic analyses, the strain represents a novel species in a new genus, for which the name Salicibibacter kimchii gen. nov., sp.
30361979	10	44	theme	CECT	1192:1195	arg1	43276T					1209:1214	= CECT 9537T; KCCM 43276T	1190:1214	= CECT 9537T; KCCM 43276T	1190:1214	nov. is proposed (= CECT 9537T; KCCM 43276T).
30361979	3	45	theme	Strain	260:265	arg1	NKC1-1T					267:273	Strain NKC1-1T	260:273	Strain NKC1-1T	260:273	Strain NKC1-1T was Gram-stain-positive, aerobic, rod-shaped, non-motile, and contained diaminopimelic acid-type murein.
30361979	2	46	theme	halophilic	159:168	arg1	NKC1-1T					206:212	A moderately halophilic and alkalitolerant bacterial strain NKC1-1T	146:212	A moderately halophilic and alkalitolerant bacterial strain NKC1-1T	146:212	A moderately halophilic and alkalitolerant bacterial strain NKC1-1T was isolated from commercial kimchi in Korea.
30361979	2	47	theme	commercial	232:241	arg1	kimchi					243:248	commercial kimchi	232:248	commercial kimchi in Korea	232:257	A moderately halophilic and alkalitolerant bacterial strain NKC1-1T was isolated from commercial kimchi in Korea.
30361979	9	48	theme	name	1129:1132	arg1	nov.					1162:1165	the name Salicibibacter kimchii gen. nov.	1125:1165	the name Salicibibacter kimchii gen. nov.	1125:1165	Based on polyphasic taxonomic evaluation with phenotypic, phylogenetic, and chemotaxonomic analyses, the strain represents a novel species in a new genus, for which the name Salicibibacter kimchii gen. nov., sp.
30361979	5	49	theme	unidentified	655:666	arg1	phospholipids					668:680	phosphatidylglycerol and unidentified phospholipids	630:680	phosphatidylglycerol and unidentified phospholipids	630:680	The major isoprenoid quinone of the isolate was menaquinone-7, and the major polar lipids were phosphatidylglycerol and unidentified phospholipids.
30361979	5	49	theme	unidentified	655:666	arg1	lipids					618:623	the major polar lipids	602:623	the major polar lipids	602:623	The major isoprenoid quinone of the isolate was menaquinone-7, and the major polar lipids were phosphatidylglycerol and unidentified phospholipids.
30361979	8	50	theme	Phylogenetic	812:823	arg1	analysis					825:832	Phylogenetic analysis	812:832	Phylogenetic analysis	812:832	Phylogenetic analysis indicated the strain to be most closely related to Geomicrobium halophilum with 92.7-92.9% 16S rRNA gene sequence similarity.
30361979	9	51	theme	Salicibibacter	1134:1147	arg1	nov.					1162:1165	the name Salicibibacter kimchii gen. nov.	1125:1165	the name Salicibibacter kimchii gen. nov.	1125:1165	Based on polyphasic taxonomic evaluation with phenotypic, phylogenetic, and chemotaxonomic analyses, the strain represents a novel species in a new genus, for which the name Salicibibacter kimchii gen. nov., sp.
30361979	0	52	dep	sp	34:35	arg1	nov.					28:31	Salicibibacter kimchii gen. nov.	0:31	Salicibibacter kimchii gen. nov.	0:31	Salicibibacter kimchii gen. nov., sp.
30361979	6	53	theme	Cell	683:686	arg1	membrane					688:695	Cell membrane	683:695	Cell membrane of the strain	683:709	Cell membrane of the strain contained iso-C17:0 and anteiso-C15:0 as the major fatty acids.
30361979	9	54	theme	novel	1085:1089	arg1	species					1091:1097	a novel species	1083:1097	a novel species	1083:1097	Based on polyphasic taxonomic evaluation with phenotypic, phylogenetic, and chemotaxonomic analyses, the strain represents a novel species in a new genus, for which the name Salicibibacter kimchii gen. nov., sp.
30361979	4	55	theme	optimal	481:487	arg1	37°C					492:495	optimal at 37°C	481:495	optimal at 37°C	481:495	Cell growth was observed in a medium containing 0-25% (w/v) NaCl (optimal at 10% [w/v]), at 20-40°C (optimal at 37°C) and pH 6.5-10.0 (optimal at pH 9.0).
30361979	4	55	theme	optimal	481:487	arg1	20-40°C					472:478	20-40°C	472:478	20-40°C (optimal at 37°C)	472:496	Cell growth was observed in a medium containing 0-25% (w/v) NaCl (optimal at 10% [w/v]), at 20-40°C (optimal at 37°C) and pH 6.5-10.0 (optimal at pH 9.0).
30361979	9	56	theme	kimchii	1149:1155	arg1	nov.					1162:1165	the name Salicibibacter kimchii gen. nov.	1125:1165	the name Salicibibacter kimchii gen. nov.	1125:1165	Based on polyphasic taxonomic evaluation with phenotypic, phylogenetic, and chemotaxonomic analyses, the strain represents a novel species in a new genus, for which the name Salicibibacter kimchii gen. nov., sp.
30361979	6	57	theme	fatty	762:766	arg1	acids					768:772	the major fatty acids	752:772	the major fatty acids	752:772	Cell membrane of the strain contained iso-C17:0 and anteiso-C15:0 as the major fatty acids.
30361979	6	57	theme	fatty	762:766	arg1	anteiso-C15:0					735:747	anteiso-C15:0	735:747	anteiso-C15:0	735:747	Cell membrane of the strain contained iso-C17:0 and anteiso-C15:0 as the major fatty acids.
30361979	6	57	theme	fatty	762:766	arg1	iso-C17:0					721:729	iso-C17:0	721:729	iso-C17:0	721:729	Cell membrane of the strain contained iso-C17:0 and anteiso-C15:0 as the major fatty acids.
30361979	1	58	theme	family	104:109	arg1	Bacillaceae					111:121	the family Bacillaceae	100:121	the family Bacillaceae	100:121	nov., a moderately halophilic and alkalitolerant bacterium in the family Bacillaceae, isolated from kimchi.
30361979	1	59	from	bacterium	87:95	arg1	Bacillaceae					111:121	the family Bacillaceae	100:121	the family Bacillaceae	100:121	nov., a moderately halophilic and alkalitolerant bacterium in the family Bacillaceae, isolated from kimchi.
30361979	2	60	theme	strain	199:204	arg1	NKC1-1T					206:212	A moderately halophilic and alkalitolerant bacterial strain NKC1-1T	146:212	A moderately halophilic and alkalitolerant bacterial strain NKC1-1T	146:212	A moderately halophilic and alkalitolerant bacterial strain NKC1-1T was isolated from commercial kimchi in Korea.
30361979	10	61	theme	9537T	1197:1201	arg1	43276T					1209:1214	= CECT 9537T; KCCM 43276T	1190:1214	= CECT 9537T; KCCM 43276T	1190:1214	nov. is proposed (= CECT 9537T; KCCM 43276T).
30361979	9	62	theme	new	1104:1106	arg1	genus					1108:1112	a new genus	1102:1112	a new genus	1102:1112	Based on polyphasic taxonomic evaluation with phenotypic, phylogenetic, and chemotaxonomic analyses, the strain represents a novel species in a new genus, for which the name Salicibibacter kimchii gen. nov., sp.
30361979	9	63	theme	chemotaxonomic	1036:1049	arg1	analyses					1051:1058	phenotypic, phylogenetic, and chemotaxonomic analyses	1006:1058	phenotypic, phylogenetic, and chemotaxonomic analyses	1006:1058	Based on polyphasic taxonomic evaluation with phenotypic, phylogenetic, and chemotaxonomic analyses, the strain represents a novel species in a new genus, for which the name Salicibibacter kimchii gen. nov., sp.
30361979	2	64	theme	bacterial	189:197	arg1	NKC1-1T					206:212	A moderately halophilic and alkalitolerant bacterial strain NKC1-1T	146:212	A moderately halophilic and alkalitolerant bacterial strain NKC1-1T	146:212	A moderately halophilic and alkalitolerant bacterial strain NKC1-1T was isolated from commercial kimchi in Korea.
30361979	10	65	theme	=	1190:1190	arg1	43276T					1209:1214	= CECT 9537T; KCCM 43276T	1190:1214	= CECT 9537T; KCCM 43276T	1190:1214	nov. is proposed (= CECT 9537T; KCCM 43276T).
30361979	4	66	theme	at	523:524	arg1	pH					526:527	optimal at pH 9.0	515:531	optimal at pH 9.0	515:531	Cell growth was observed in a medium containing 0-25% (w/v) NaCl (optimal at 10% [w/v]), at 20-40°C (optimal at 37°C) and pH 6.5-10.0 (optimal at pH 9.0).
30361979	4	66	theme	at	523:524	arg1	pH					502:503	pH 6.5-10.0	502:512	pH 6.5-10.0 (optimal at pH 9.0)	502:532	Cell growth was observed in a medium containing 0-25% (w/v) NaCl (optimal at 10% [w/v]), at 20-40°C (optimal at 37°C) and pH 6.5-10.0 (optimal at pH 9.0).
30361979	4	67	theme	%	432:432	arg1	NaCl					440:443	0-25% (w/v) NaCl	428:443	0-25% (w/v) NaCl (optimal at 10% [w/v])	428:466	Cell growth was observed in a medium containing 0-25% (w/v) NaCl (optimal at 10% [w/v]), at 20-40°C (optimal at 37°C) and pH 6.5-10.0 (optimal at pH 9.0).
30361979	7	68	theme	mol	806:808	arg1	%					809:809	45.2 mol%	801:809	45.2 mol%	801:809	Its DNA G + C content was 45.2 mol%.
30361979	8	69	theme	16S	925:927	arg1	similarity					948:957	92.7-92.9% 16S rRNA gene sequence similarity	914:957	92.7-92.9% 16S rRNA gene sequence similarity	914:957	Phylogenetic analysis indicated the strain to be most closely related to Geomicrobium halophilum with 92.7-92.9% 16S rRNA gene sequence similarity.
30361979	4	70	located	observed	396:403	arg1	37°C					492:495	optimal at 37°C	481:495	optimal at 37°C	481:495	Cell growth was observed in a medium containing 0-25% (w/v) NaCl (optimal at 10% [w/v]), at 20-40°C (optimal at 37°C) and pH 6.5-10.0 (optimal at pH 9.0).
30361979	4	70	located	observed	396:403	arg2	growth					385:390	Cell growth	380:390	Cell growth	380:390	Cell growth was observed in a medium containing 0-25% (w/v) NaCl (optimal at 10% [w/v]), at 20-40°C (optimal at 37°C) and pH 6.5-10.0 (optimal at pH 9.0).
30361979	4	70	located	observed	396:403	arg1	pH					526:527	optimal at pH 9.0	515:531	optimal at pH 9.0	515:531	Cell growth was observed in a medium containing 0-25% (w/v) NaCl (optimal at 10% [w/v]), at 20-40°C (optimal at 37°C) and pH 6.5-10.0 (optimal at pH 9.0).
30361979	4	70	located	observed	396:403	arg1	20-40°C					472:478	20-40°C	472:478	20-40°C (optimal at 37°C)	472:496	Cell growth was observed in a medium containing 0-25% (w/v) NaCl (optimal at 10% [w/v]), at 20-40°C (optimal at 37°C) and pH 6.5-10.0 (optimal at pH 9.0).
30361979	4	70	located	observed	396:403	arg1	medium					410:415	a medium	408:415	a medium containing 0-25% (w/v) NaCl (optimal at 10% [w/v])	408:466	Cell growth was observed in a medium containing 0-25% (w/v) NaCl (optimal at 10% [w/v]), at 20-40°C (optimal at 37°C) and pH 6.5-10.0 (optimal at pH 9.0).
30361979	4	70	located	observed	396:403	arg1	pH					502:503	pH 6.5-10.0	502:512	pH 6.5-10.0 (optimal at pH 9.0)	502:532	Cell growth was observed in a medium containing 0-25% (w/v) NaCl (optimal at 10% [w/v]), at 20-40°C (optimal at 37°C) and pH 6.5-10.0 (optimal at pH 9.0).
30361979	5	71	theme	isoprenoid	545:554	arg1	menaquinone-7					583:595	menaquinone-7	583:595	menaquinone-7	583:595	The major isoprenoid quinone of the isolate was menaquinone-7, and the major polar lipids were phosphatidylglycerol and unidentified phospholipids.
30361979	5	71	theme	isoprenoid	545:554	arg1	quinone					556:562	The major isoprenoid quinone	535:562	The major isoprenoid quinone of the isolate	535:577	The major isoprenoid quinone of the isolate was menaquinone-7, and the major polar lipids were phosphatidylglycerol and unidentified phospholipids.
29537361	9	0	theme	YIM	946:948	arg1	75904T					950:955	Amycolatopsis dongchuanensis YIM 75904T	917:955	Amycolatopsis dongchuanensis YIM 75904T (98.06 %)	917:965	Phylogenetic analysis using 16S rRNA gene sequences revealed that strain DH51B-4-3T should be classified in the genus Amycolatopsis and closely related to Amycolatopsis dongchuanensis YIM 75904T (98.06 %) and Amycolatopsis sacchari DSM 44468T (97.77 %).
29537361	9	0	theme	YIM	946:948	arg1	%					964:964	98.06 %	958:964	98.06 %	958:964	Phylogenetic analysis using 16S rRNA gene sequences revealed that strain DH51B-4-3T should be classified in the genus Amycolatopsis and closely related to Amycolatopsis dongchuanensis YIM 75904T (98.06 %) and Amycolatopsis sacchari DSM 44468T (97.77 %).
29537361	11	1	dep	data	1254:1257	arg1	basis					1214:1218	basis	1214:1218	basis	1214:1218	On the basis of these phenotypic and genotypic data, this strain represents a novel species, for which the name Amycolatopsis rhizosphaerae sp.
29537361	11	1	dep	data	1254:1257	arg1	the					1210:1212	the	1210:1212	the	1210:1212	On the basis of these phenotypic and genotypic data, this strain represents a novel species, for which the name Amycolatopsis rhizosphaerae sp.
29537361	7	2	dep	iso-C16 	681:688	arg1	 0					690:691	 0	690:691	 0	690:691	The main cellular fatty acids were iso-C16 : 0 and cyclo-C17 : 0.
29537361	7	2	dep	iso-C16 	681:688	arg1	 0					708:709	 0	708:709	iso-C16 : 0 and cyclo-C17 : 0	681:709	The main cellular fatty acids were iso-C16 : 0 and cyclo-C17 : 0.
29537361	7	2	dep	iso-C16 	681:688	arg1	cyclo-C17 					697:706	cyclo-C17 	697:706	cyclo-C17 	697:706	The main cellular fatty acids were iso-C16 : 0 and cyclo-C17 : 0.
29537361	3	3	theme	meso-diaminopimelic	301:319	arg1	acid					321:324	meso-diaminopimelic acid	301:324	meso-diaminopimelic acid in the cell-wall peptidoglycan	301:355	The isolate contained meso-diaminopimelic acid in the cell-wall peptidoglycan.
29537361	2	4	theme	strain	108:113	arg1	DH51B-4-3T					115:124	strain DH51B-4-3T	108:124	strain DH51B-4-3T	108:124	A novel actinomycete, designated strain DH51B-4-3T, which formed long chains of spherical spores borne on the tip of sporophores arising from the aerial mycelium, was isolated from rice rhizosphere soil.
29537361	10	5	theme	DNA-DNA	1030:1036	arg1	hybridization					1038:1050	DNA-DNA hybridization	1030:1050	DNA-DNA hybridization	1030:1050	The result of DNA-DNA hybridization and some physiological and biochemical properties indicated that strain DH51B-4-3T could be readily distinguished from its closest phylogenetic relatives.
29537361	4	6	theme	DH51B-4-3T	390:399	arg1	sugars					373:378	The whole-cell sugars	358:378	The whole-cell sugars of strain DH51B-4-3T	358:399	The whole-cell sugars of strain DH51B-4-3T were arabinose, galactose, glucose, rhamnose and ribose.
29537361	4	6	theme	DH51B-4-3T	390:399	arg1	arabinose					406:414	arabinose	406:414	arabinose	406:414	The whole-cell sugars of strain DH51B-4-3T were arabinose, galactose, glucose, rhamnose and ribose.
29537361	9	7	theme	strain	828:833	arg1	DH51B-4-3T					835:844	strain DH51B-4-3T	828:844	strain DH51B-4-3T	828:844	Phylogenetic analysis using 16S rRNA gene sequences revealed that strain DH51B-4-3T should be classified in the genus Amycolatopsis and closely related to Amycolatopsis dongchuanensis YIM 75904T (98.06 %) and Amycolatopsis sacchari DSM 44468T (97.77 %).
29537361	7	8	theme	fatty	664:668	arg1	iso-C16 					681:688	iso-C16 	681:688	iso-C16 	681:688	The main cellular fatty acids were iso-C16 : 0 and cyclo-C17 : 0.
29537361	7	8	theme	fatty	664:668	arg1	acids					670:674	The main cellular fatty acids	646:674	The main cellular fatty acids	646:674	The main cellular fatty acids were iso-C16 : 0 and cyclo-C17 : 0.
29537361	8	9	theme	68.2 mol	751:758	arg1	%					759:759	68.2 mol%	751:759	68.2 mol%	751:759	The G+C content of the genomic DNA was 68.2 mol%.
29537361	8	9	theme	68.2 mol	751:758	arg1	G+C content					716:726	The G+C content	712:726	The G+C content of the genomic DNA	712:745	The G+C content of the genomic DNA was 68.2 mol%.
29537361	4	10	theme	strain	383:388	arg1	DH51B-4-3T					390:399	strain DH51B-4-3T	383:399	strain DH51B-4-3T	383:399	The whole-cell sugars of strain DH51B-4-3T were arabinose, galactose, glucose, rhamnose and ribose.
29537361	2	11	theme	rhizosphere	261:271	arg1	soil					273:276	rice rhizosphere soil	256:276	rice rhizosphere soil	256:276	A novel actinomycete, designated strain DH51B-4-3T, which formed long chains of spherical spores borne on the tip of sporophores arising from the aerial mycelium, was isolated from rice rhizosphere soil.
29537361	10	12	theme	strain	1117:1122	arg1	DH51B-4-3T					1124:1133	strain DH51B-4-3T	1117:1133	strain DH51B-4-3T	1117:1133	The result of DNA-DNA hybridization and some physiological and biochemical properties indicated that strain DH51B-4-3T could be readily distinguished from its closest phylogenetic relatives.
29537361	1	13	theme	rhizosphere	57:67	arg1	soil					69:72	rice rhizosphere soil	52:72	rice rhizosphere soil	52:72	nov., isolated from rice rhizosphere soil.
29537361	11	14	theme	phenotypic	1229:1238	arg1	data					1254:1257	these phenotypic and genotypic data	1223:1257	these phenotypic and genotypic data	1223:1257	On the basis of these phenotypic and genotypic data, this strain represents a novel species, for which the name Amycolatopsis rhizosphaerae sp.
29537361	2	15	theme	rice	256:259	arg1	soil					273:276	rice rhizosphere soil	256:276	rice rhizosphere soil	256:276	A novel actinomycete, designated strain DH51B-4-3T, which formed long chains of spherical spores borne on the tip of sporophores arising from the aerial mycelium, was isolated from rice rhizosphere soil.
29537361	10	16	theme	physiological	1061:1073	arg1	properties					1091:1100	some physiological and biochemical properties	1056:1100	some physiological and biochemical properties	1056:1100	The result of DNA-DNA hybridization and some physiological and biochemical properties indicated that strain DH51B-4-3T could be readily distinguished from its closest phylogenetic relatives.
29537361	2	17	theme	long	140:143	arg1	chains					145:150	long chains	140:150	long chains of spherical spores borne on the tip of sporophores arising from the aerial mycelium	140:235	A novel actinomycete, designated strain DH51B-4-3T, which formed long chains of spherical spores borne on the tip of sporophores arising from the aerial mycelium, was isolated from rice rhizosphere soil.
29537361	10	18	theme	closest	1175:1181	arg1	relatives					1196:1204	its closest phylogenetic relatives	1171:1204	its closest phylogenetic relatives	1171:1204	The result of DNA-DNA hybridization and some physiological and biochemical properties indicated that strain DH51B-4-3T could be readily distinguished from its closest phylogenetic relatives.
29537361	5	19	theme	cell	483:486	arg1	membrane					488:495	the cell membrane	479:495	the cell membrane	479:495	The phospholipids in the cell membrane were phosphatidylethanolamine, phosphatidyl methylethanolamine, diphosphatidylglycerol and phosphatidylinositol.
29537361	9	20	theme	16S	790:792	arg1	sequences					804:812	16S rRNA gene sequences	790:812	16S rRNA gene sequences	790:812	Phylogenetic analysis using 16S rRNA gene sequences revealed that strain DH51B-4-3T should be classified in the genus Amycolatopsis and closely related to Amycolatopsis dongchuanensis YIM 75904T (98.06 %) and Amycolatopsis sacchari DSM 44468T (97.77 %).
29537361	3	21	theme	cell-wall	333:341	arg1	peptidoglycan					343:355	the cell-wall peptidoglycan	329:355	the cell-wall peptidoglycan	329:355	The isolate contained meso-diaminopimelic acid in the cell-wall peptidoglycan.
29537361	11	22	theme	name	1314:1317	arg1	sp					1347:1348	the name Amycolatopsis rhizosphaerae sp	1310:1348	the name Amycolatopsis rhizosphaerae sp	1310:1348	On the basis of these phenotypic and genotypic data, this strain represents a novel species, for which the name Amycolatopsis rhizosphaerae sp.
29537361	4	23	theme	whole-cell	362:371	arg1	sugars					373:378	The whole-cell sugars	358:378	The whole-cell sugars of strain DH51B-4-3T	358:399	The whole-cell sugars of strain DH51B-4-3T were arabinose, galactose, glucose, rhamnose and ribose.
29537361	4	23	theme	whole-cell	362:371	arg1	arabinose					406:414	arabinose	406:414	arabinose	406:414	The whole-cell sugars of strain DH51B-4-3T were arabinose, galactose, glucose, rhamnose and ribose.
29537361	9	24	theme	Amycolatopsis	971:983	arg1	44468T					998:1003	Amycolatopsis sacchari DSM 44468T	971:1003	Amycolatopsis sacchari DSM 44468T (97.77 %)	971:1013	Phylogenetic analysis using 16S rRNA gene sequences revealed that strain DH51B-4-3T should be classified in the genus Amycolatopsis and closely related to Amycolatopsis dongchuanensis YIM 75904T (98.06 %) and Amycolatopsis sacchari DSM 44468T (97.77 %).
29537361	9	24	theme	Amycolatopsis	971:983	arg1	%					1012:1012	97.77 %	1006:1012	97.77 %	1006:1012	Phylogenetic analysis using 16S rRNA gene sequences revealed that strain DH51B-4-3T should be classified in the genus Amycolatopsis and closely related to Amycolatopsis dongchuanensis YIM 75904T (98.06 %) and Amycolatopsis sacchari DSM 44468T (97.77 %).
29537361	2	25	attach	isolated	242:249	arg2	actinomycete					83:94	A novel actinomycete	75:94	A novel actinomycete	75:94	A novel actinomycete, designated strain DH51B-4-3T, which formed long chains of spherical spores borne on the tip of sporophores arising from the aerial mycelium, was isolated from rice rhizosphere soil.
29537361	2	25	attach	isolated	242:249	arg1	soil					273:276	rice rhizosphere soil	256:276	rice rhizosphere soil	256:276	A novel actinomycete, designated strain DH51B-4-3T, which formed long chains of spherical spores borne on the tip of sporophores arising from the aerial mycelium, was isolated from rice rhizosphere soil.
29537361	0	26	theme	rhizosphaerae	14:26	arg1	sp					28:29	Amycolatopsis rhizosphaerae sp	0:29	Amycolatopsis rhizosphaerae sp.	0:30	Amycolatopsis rhizosphaerae sp.
29537361	10	27	theme	properties	1091:1100	arg1	result					1020:1025	The result	1016:1025	The result of DNA-DNA hybridization and some physiological and biochemical properties	1016:1100	The result of DNA-DNA hybridization and some physiological and biochemical properties indicated that strain DH51B-4-3T could be readily distinguished from its closest phylogenetic relatives.
29537361	7	28	theme	main	650:653	arg1	iso-C16 					681:688	iso-C16 	681:688	iso-C16 	681:688	The main cellular fatty acids were iso-C16 : 0 and cyclo-C17 : 0.
29537361	7	28	theme	main	650:653	arg1	acids					670:674	The main cellular fatty acids	646:674	The main cellular fatty acids	646:674	The main cellular fatty acids were iso-C16 : 0 and cyclo-C17 : 0.
29537361	0	29	theme	Amycolatopsis	0:12	arg1	sp					28:29	Amycolatopsis rhizosphaerae sp	0:29	Amycolatopsis rhizosphaerae sp.	0:30	Amycolatopsis rhizosphaerae sp.
29537361	13	30	theme	type	1373:1376	arg1	strain					1378:1383	The type strain	1369:1383	The type strain	1369:1383	The type strain is DH51B-4-3T (=TBRC 6029T=NBRC 112509T).
29537361	13	30	theme	type	1373:1376	arg1	DH51B-4-3T					1388:1397	DH51B-4-3T	1388:1397	DH51B-4-3T (=TBRC 6029T=NBRC 112509T)	1388:1424	The type strain is DH51B-4-3T (=TBRC 6029T=NBRC 112509T).
29537361	9	31	theme	sacchari	985:992	arg1	44468T					998:1003	Amycolatopsis sacchari DSM 44468T	971:1003	Amycolatopsis sacchari DSM 44468T (97.77 %)	971:1013	Phylogenetic analysis using 16S rRNA gene sequences revealed that strain DH51B-4-3T should be classified in the genus Amycolatopsis and closely related to Amycolatopsis dongchuanensis YIM 75904T (98.06 %) and Amycolatopsis sacchari DSM 44468T (97.77 %).
29537361	9	31	theme	sacchari	985:992	arg1	%					1012:1012	97.77 %	1006:1012	97.77 %	1006:1012	Phylogenetic analysis using 16S rRNA gene sequences revealed that strain DH51B-4-3T should be classified in the genus Amycolatopsis and closely related to Amycolatopsis dongchuanensis YIM 75904T (98.06 %) and Amycolatopsis sacchari DSM 44468T (97.77 %).
29537361	11	32	theme	rhizosphaerae	1333:1345	arg1	sp					1347:1348	the name Amycolatopsis rhizosphaerae sp	1310:1348	the name Amycolatopsis rhizosphaerae sp	1310:1348	On the basis of these phenotypic and genotypic data, this strain represents a novel species, for which the name Amycolatopsis rhizosphaerae sp.
29537361	11	33	theme	genotypic	1244:1252	arg1	data					1254:1257	these phenotypic and genotypic data	1223:1257	these phenotypic and genotypic data	1223:1257	On the basis of these phenotypic and genotypic data, this strain represents a novel species, for which the name Amycolatopsis rhizosphaerae sp.
29537361	9	34	theme	DSM	994:996	arg1	44468T					998:1003	Amycolatopsis sacchari DSM 44468T	971:1003	Amycolatopsis sacchari DSM 44468T (97.77 %)	971:1013	Phylogenetic analysis using 16S rRNA gene sequences revealed that strain DH51B-4-3T should be classified in the genus Amycolatopsis and closely related to Amycolatopsis dongchuanensis YIM 75904T (98.06 %) and Amycolatopsis sacchari DSM 44468T (97.77 %).
29537361	9	34	theme	DSM	994:996	arg1	%					1012:1012	97.77 %	1006:1012	97.77 %	1006:1012	Phylogenetic analysis using 16S rRNA gene sequences revealed that strain DH51B-4-3T should be classified in the genus Amycolatopsis and closely related to Amycolatopsis dongchuanensis YIM 75904T (98.06 %) and Amycolatopsis sacchari DSM 44468T (97.77 %).
29537361	13	35	theme	=TBRC	1400:1404	arg1	112509T					1417:1423	=TBRC 6029T=NBRC 112509T	1400:1423	=TBRC 6029T=NBRC 112509T	1400:1423	The type strain is DH51B-4-3T (=TBRC 6029T=NBRC 112509T).
29537361	13	35	theme	=TBRC	1400:1404	arg1	DH51B-4-3T					1388:1397	DH51B-4-3T	1388:1397	DH51B-4-3T (=TBRC 6029T=NBRC 112509T)	1388:1424	The type strain is DH51B-4-3T (=TBRC 6029T=NBRC 112509T).
29537361	11	36	theme	novel	1285:1289	arg1	species					1291:1297	a novel species	1283:1297	a novel species	1283:1297	On the basis of these phenotypic and genotypic data, this strain represents a novel species, for which the name Amycolatopsis rhizosphaerae sp.
29537361	13	37	theme	6029T=NBRC	1406:1415	arg1	112509T					1417:1423	=TBRC 6029T=NBRC 112509T	1400:1423	=TBRC 6029T=NBRC 112509T	1400:1423	The type strain is DH51B-4-3T (=TBRC 6029T=NBRC 112509T).
29537361	13	37	theme	6029T=NBRC	1406:1415	arg1	DH51B-4-3T					1388:1397	DH51B-4-3T	1388:1397	DH51B-4-3T (=TBRC 6029T=NBRC 112509T)	1388:1424	The type strain is DH51B-4-3T (=TBRC 6029T=NBRC 112509T).
29537361	9	38	theme	genus	874:878	arg1	Amycolatopsis					880:892	the genus Amycolatopsis	870:892	the genus Amycolatopsis	870:892	Phylogenetic analysis using 16S rRNA gene sequences revealed that strain DH51B-4-3T should be classified in the genus Amycolatopsis and closely related to Amycolatopsis dongchuanensis YIM 75904T (98.06 %) and Amycolatopsis sacchari DSM 44468T (97.77 %).
29537361	2	39	theme	spores	165:170	arg1	chains					145:150	long chains	140:150	long chains of spherical spores borne on the tip of sporophores arising from the aerial mycelium	140:235	A novel actinomycete, designated strain DH51B-4-3T, which formed long chains of spherical spores borne on the tip of sporophores arising from the aerial mycelium, was isolated from rice rhizosphere soil.
29537361	5	40	from	phospholipids	462:474	arg1	membrane					488:495	the cell membrane	479:495	the cell membrane	479:495	The phospholipids in the cell membrane were phosphatidylethanolamine, phosphatidyl methylethanolamine, diphosphatidylglycerol and phosphatidylinositol.
29537361	6	41	theme	major	614:618	arg1	MK-9					636:639	MK-9	636:639	MK-9(H4)	636:643	The major menaquinone was MK-9(H4).
29537361	6	41	theme	major	614:618	arg1	menaquinone					620:630	The major menaquinone	610:630	The major menaquinone	610:630	The major menaquinone was MK-9(H4).
29537361	8	42	theme	DNA	743:745	arg1	%					759:759	68.2 mol%	751:759	68.2 mol%	751:759	The G+C content of the genomic DNA was 68.2 mol%.
29537361	8	42	theme	DNA	743:745	arg1	G+C content					716:726	The G+C content	712:726	The G+C content of the genomic DNA	712:745	The G+C content of the genomic DNA was 68.2 mol%.
29537361	2	43	theme	spherical	155:163	arg1	spores					165:170	spherical spores	155:170	spherical spores borne on the tip of sporophores arising from the aerial mycelium	155:235	A novel actinomycete, designated strain DH51B-4-3T, which formed long chains of spherical spores borne on the tip of sporophores arising from the aerial mycelium, was isolated from rice rhizosphere soil.
29537361	11	44	theme	Amycolatopsis	1319:1331	arg1	sp					1347:1348	the name Amycolatopsis rhizosphaerae sp	1310:1348	the name Amycolatopsis rhizosphaerae sp	1310:1348	On the basis of these phenotypic and genotypic data, this strain represents a novel species, for which the name Amycolatopsis rhizosphaerae sp.
29537361	8	45	theme	genomic	735:741	arg1	DNA					743:745	the genomic DNA	731:745	the genomic DNA	731:745	The G+C content of the genomic DNA was 68.2 mol%.
29537361	9	46	theme	Phylogenetic	762:773	arg1	analysis					775:782	Phylogenetic analysis	762:782	Phylogenetic analysis using 16S rRNA gene sequences	762:812	Phylogenetic analysis using 16S rRNA gene sequences revealed that strain DH51B-4-3T should be classified in the genus Amycolatopsis and closely related to Amycolatopsis dongchuanensis YIM 75904T (98.06 %) and Amycolatopsis sacchari DSM 44468T (97.77 %).
29537361	2	47	theme	novel	77:81	arg1	actinomycete					83:94	A novel actinomycete	75:94	A novel actinomycete	75:94	A novel actinomycete, designated strain DH51B-4-3T, which formed long chains of spherical spores borne on the tip of sporophores arising from the aerial mycelium, was isolated from rice rhizosphere soil.
29537361	2	48	theme	sporophores	192:202	arg1	tip					185:187	the tip	181:187	the tip of sporophores arising from the aerial mycelium	181:235	A novel actinomycete, designated strain DH51B-4-3T, which formed long chains of spherical spores borne on the tip of sporophores arising from the aerial mycelium, was isolated from rice rhizosphere soil.
29537361	9	49	theme	gene	799:802	arg1	sequences					804:812	16S rRNA gene sequences	790:812	16S rRNA gene sequences	790:812	Phylogenetic analysis using 16S rRNA gene sequences revealed that strain DH51B-4-3T should be classified in the genus Amycolatopsis and closely related to Amycolatopsis dongchuanensis YIM 75904T (98.06 %) and Amycolatopsis sacchari DSM 44468T (97.77 %).
29537361	10	50	theme	hybridization	1038:1050	arg1	result					1020:1025	The result	1016:1025	The result of DNA-DNA hybridization and some physiological and biochemical properties	1016:1100	The result of DNA-DNA hybridization and some physiological and biochemical properties indicated that strain DH51B-4-3T could be readily distinguished from its closest phylogenetic relatives.
29537361	7	51	theme	cellular	655:662	arg1	iso-C16 					681:688	iso-C16 	681:688	iso-C16 	681:688	The main cellular fatty acids were iso-C16 : 0 and cyclo-C17 : 0.
29537361	7	51	theme	cellular	655:662	arg1	acids					670:674	The main cellular fatty acids	646:674	The main cellular fatty acids	646:674	The main cellular fatty acids were iso-C16 : 0 and cyclo-C17 : 0.
29537361	3	52	from	acid	321:324	arg1	peptidoglycan					343:355	the cell-wall peptidoglycan	329:355	the cell-wall peptidoglycan	329:355	The isolate contained meso-diaminopimelic acid in the cell-wall peptidoglycan.
29537361	1	53	theme	rice	52:55	arg1	soil					69:72	rice rhizosphere soil	52:72	rice rhizosphere soil	52:72	nov., isolated from rice rhizosphere soil.
29537361	9	54	theme	Amycolatopsis	917:929	arg1	75904T					950:955	Amycolatopsis dongchuanensis YIM 75904T	917:955	Amycolatopsis dongchuanensis YIM 75904T (98.06 %)	917:965	Phylogenetic analysis using 16S rRNA gene sequences revealed that strain DH51B-4-3T should be classified in the genus Amycolatopsis and closely related to Amycolatopsis dongchuanensis YIM 75904T (98.06 %) and Amycolatopsis sacchari DSM 44468T (97.77 %).
29537361	9	54	theme	Amycolatopsis	917:929	arg1	%					964:964	98.06 %	958:964	98.06 %	958:964	Phylogenetic analysis using 16S rRNA gene sequences revealed that strain DH51B-4-3T should be classified in the genus Amycolatopsis and closely related to Amycolatopsis dongchuanensis YIM 75904T (98.06 %) and Amycolatopsis sacchari DSM 44468T (97.77 %).
29537361	9	55	theme	rRNA	794:797	arg1	sequences					804:812	16S rRNA gene sequences	790:812	16S rRNA gene sequences	790:812	Phylogenetic analysis using 16S rRNA gene sequences revealed that strain DH51B-4-3T should be classified in the genus Amycolatopsis and closely related to Amycolatopsis dongchuanensis YIM 75904T (98.06 %) and Amycolatopsis sacchari DSM 44468T (97.77 %).
29537361	3	56	contain	contained	291:299	arg2	acid					321:324	meso-diaminopimelic acid	301:324	meso-diaminopimelic acid in the cell-wall peptidoglycan	301:355	The isolate contained meso-diaminopimelic acid in the cell-wall peptidoglycan.
29537361	3	56	contain	contained	291:299	arg1	isolate					283:289	The isolate	279:289	The isolate	279:289	The isolate contained meso-diaminopimelic acid in the cell-wall peptidoglycan.
29537361	9	57	theme	dongchuanensis	931:944	arg1	75904T					950:955	Amycolatopsis dongchuanensis YIM 75904T	917:955	Amycolatopsis dongchuanensis YIM 75904T (98.06 %)	917:965	Phylogenetic analysis using 16S rRNA gene sequences revealed that strain DH51B-4-3T should be classified in the genus Amycolatopsis and closely related to Amycolatopsis dongchuanensis YIM 75904T (98.06 %) and Amycolatopsis sacchari DSM 44468T (97.77 %).
29537361	9	57	theme	dongchuanensis	931:944	arg1	%					964:964	98.06 %	958:964	98.06 %	958:964	Phylogenetic analysis using 16S rRNA gene sequences revealed that strain DH51B-4-3T should be classified in the genus Amycolatopsis and closely related to Amycolatopsis dongchuanensis YIM 75904T (98.06 %) and Amycolatopsis sacchari DSM 44468T (97.77 %).
29537361	10	58	theme	biochemical	1079:1089	arg1	properties					1091:1100	some physiological and biochemical properties	1056:1100	some physiological and biochemical properties	1056:1100	The result of DNA-DNA hybridization and some physiological and biochemical properties indicated that strain DH51B-4-3T could be readily distinguished from its closest phylogenetic relatives.
29537361	2	59	theme	aerial	221:226	arg1	mycelium					228:235	the aerial mycelium	217:235	the aerial mycelium	217:235	A novel actinomycete, designated strain DH51B-4-3T, which formed long chains of spherical spores borne on the tip of sporophores arising from the aerial mycelium, was isolated from rice rhizosphere soil.
29537361	5	60	theme	phosphatidyl	528:539	arg1	methylethanolamine					541:558	phosphatidyl methylethanolamine	528:558	phosphatidyl methylethanolamine	528:558	The phospholipids in the cell membrane were phosphatidylethanolamine, phosphatidyl methylethanolamine, diphosphatidylglycerol and phosphatidylinositol.
29537361	10	61	theme	phylogenetic	1183:1194	arg1	relatives					1196:1204	its closest phylogenetic relatives	1171:1204	its closest phylogenetic relatives	1171:1204	The result of DNA-DNA hybridization and some physiological and biochemical properties indicated that strain DH51B-4-3T could be readily distinguished from its closest phylogenetic relatives.
30042191	5	0	theme	phylum	893:898	arg1	Firmicutes					900:909	the phylum Firmicutes	889:909	the phylum Firmicutes	889:909	Expression of the MAFF system facilitates symbiosis with other members of the phylum Firmicutes and promotes protection from a chemically induced model of colitis.
30042191	6	1	theme	novel	997:1001	arg1	mechanism					1003:1011	a novel mechanism	995:1011	a novel mechanism by which IgA promotes symbiosis and colonic homeostasis	995:1067	Our data reveal a novel mechanism by which IgA promotes symbiosis and colonic homeostasis.
30042191	4	2	located	detected	700:707	arg1	humans					684:689	humans	684:689	humans	684:689	In both mice and humans, MAFF is detected predominantly in mucus-resident bacteria, and its expression requires the presence of complex microbiota.
30042191	4	2	located	detected	700:707	arg1	bacteria					741:748	mucus-resident bacteria	726:748	mucus-resident bacteria	726:748	In both mice and humans, MAFF is detected predominantly in mucus-resident bacteria, and its expression requires the presence of complex microbiota.
30042191	4	2	located	detected	700:707	arg1	mice					675:678	mice	675:678	mice	675:678	In both mice and humans, MAFF is detected predominantly in mucus-resident bacteria, and its expression requires the presence of complex microbiota.
30042191	4	2	located	detected	700:707	arg2	MAFF					692:695	MAFF	692:695	MAFF	692:695	In both mice and humans, MAFF is detected predominantly in mucus-resident bacteria, and its expression requires the presence of complex microbiota.
30042191	5	3	theme	Firmicutes	900:909	arg1	members					878:884	other members	872:884	other members of the phylum Firmicutes	872:909	Expression of the MAFF system facilitates symbiosis with other members of the phylum Firmicutes and promotes protection from a chemically induced model of colitis.
30042191	3	4	theme	loci	534:537	arg1	expression					493:502	the expression	489:502	the expression of polysaccharide utilization loci (PUL), including a functionally uncharacterized molecular family provisionally named Mucus-Associated Functional Factor (MAFF)	489:664	In vivo, IgA alters the expression of polysaccharide utilization loci (PUL), including a functionally uncharacterized molecular family provisionally named Mucus-Associated Functional Factor (MAFF).
30042191	1	5	theme	such	248:251	arg1	function					269:276	such homeostatic IgA function	248:276	such homeostatic IgA function	248:276	Immunoglobulin A (IgA) promotes health by regulating the composition and function of gut microbiota, but the molecular requirements for such homeostatic IgA function remain unknown.
30042191	5	6	theme	colitis	970:976	arg1	model					961:965	a chemically induced model	940:965	a chemically induced model of colitis	940:976	Expression of the MAFF system facilitates symbiosis with other members of the phylum Firmicutes and promotes protection from a chemically induced model of colitis.
30042191	1	7	theme	homeostatic	253:263	arg1	function					269:276	such homeostatic IgA function	248:276	such homeostatic IgA function	248:276	Immunoglobulin A (IgA) promotes health by regulating the composition and function of gut microbiota, but the molecular requirements for such homeostatic IgA function remain unknown.
30042191	2	8	theme	monoclonal	331:340	arg1	IgA					342:344	a heavily glycosylated monoclonal IgA	308:344	a heavily glycosylated monoclonal IgA recognizing ovalbumin	308:366	We found that a heavily glycosylated monoclonal IgA recognizing ovalbumin coats Bacteroides thetaiotaomicron (B. theta), a prominent gut symbiont of the phylum Bacteroidetes.
30042191	3	9	theme	polysaccharide	507:520	arg1	loci					534:537	polysaccharide utilization loci	507:537	polysaccharide utilization loci (PUL)	507:543	In vivo, IgA alters the expression of polysaccharide utilization loci (PUL), including a functionally uncharacterized molecular family provisionally named Mucus-Associated Functional Factor (MAFF).
30042191	3	9	theme	polysaccharide	507:520	arg1	family					597:602	a functionally uncharacterized molecular family	556:602	a functionally uncharacterized molecular family provisionally named Mucus-Associated Functional Factor (MAFF)	556:664	In vivo, IgA alters the expression of polysaccharide utilization loci (PUL), including a functionally uncharacterized molecular family provisionally named Mucus-Associated Functional Factor (MAFF).
30042191	3	9	theme	polysaccharide	507:520	arg1	PUL					540:542	PUL	540:542	PUL	540:542	In vivo, IgA alters the expression of polysaccharide utilization loci (PUL), including a functionally uncharacterized molecular family provisionally named Mucus-Associated Functional Factor (MAFF).
30042191	2	10	theme	B.	404:405	arg1	thetaiotaomicron					386:401	Bacteroides thetaiotaomicron	374:401	Bacteroides thetaiotaomicron (B. theta)	374:412	We found that a heavily glycosylated monoclonal IgA recognizing ovalbumin coats Bacteroides thetaiotaomicron (B. theta), a prominent gut symbiont of the phylum Bacteroidetes.
30042191	2	10	theme	B.	404:405	arg1	theta					407:411	B. theta	404:411	B. theta	404:411	We found that a heavily glycosylated monoclonal IgA recognizing ovalbumin coats Bacteroides thetaiotaomicron (B. theta), a prominent gut symbiont of the phylum Bacteroidetes.
30042191	3	11	theme	utilization	522:532	arg1	loci					534:537	polysaccharide utilization loci	507:537	polysaccharide utilization loci (PUL)	507:543	In vivo, IgA alters the expression of polysaccharide utilization loci (PUL), including a functionally uncharacterized molecular family provisionally named Mucus-Associated Functional Factor (MAFF).
30042191	3	11	theme	utilization	522:532	arg1	family					597:602	a functionally uncharacterized molecular family	556:602	a functionally uncharacterized molecular family provisionally named Mucus-Associated Functional Factor (MAFF)	556:664	In vivo, IgA alters the expression of polysaccharide utilization loci (PUL), including a functionally uncharacterized molecular family provisionally named Mucus-Associated Functional Factor (MAFF).
30042191	3	11	theme	utilization	522:532	arg1	PUL					540:542	PUL	540:542	PUL	540:542	In vivo, IgA alters the expression of polysaccharide utilization loci (PUL), including a functionally uncharacterized molecular family provisionally named Mucus-Associated Functional Factor (MAFF).
30042191	4	12	theme	mucus-resident	726:739	arg1	bacteria					741:748	mucus-resident bacteria	726:748	mucus-resident bacteria	726:748	In both mice and humans, MAFF is detected predominantly in mucus-resident bacteria, and its expression requires the presence of complex microbiota.
30042191	5	13	theme	other	872:876	arg1	members					878:884	other members	872:884	other members of the phylum Firmicutes	872:909	Expression of the MAFF system facilitates symbiosis with other members of the phylum Firmicutes and promotes protection from a chemically induced model of colitis.
30042191	1	14	theme	IgA	265:267	arg1	function					269:276	such homeostatic IgA function	248:276	such homeostatic IgA function	248:276	Immunoglobulin A (IgA) promotes health by regulating the composition and function of gut microbiota, but the molecular requirements for such homeostatic IgA function remain unknown.
30042191	2	15	theme	Bacteroides	374:384	arg1	symbiont					431:438	a prominent gut symbiont	415:438	a prominent gut symbiont of the phylum Bacteroidetes	415:466	We found that a heavily glycosylated monoclonal IgA recognizing ovalbumin coats Bacteroides thetaiotaomicron (B. theta), a prominent gut symbiont of the phylum Bacteroidetes.
30042191	2	15	theme	Bacteroides	374:384	arg1	thetaiotaomicron					386:401	Bacteroides thetaiotaomicron	374:401	Bacteroides thetaiotaomicron (B. theta)	374:412	We found that a heavily glycosylated monoclonal IgA recognizing ovalbumin coats Bacteroides thetaiotaomicron (B. theta), a prominent gut symbiont of the phylum Bacteroidetes.
30042191	2	15	theme	Bacteroides	374:384	arg1	theta					407:411	B. theta	404:411	B. theta	404:411	We found that a heavily glycosylated monoclonal IgA recognizing ovalbumin coats Bacteroides thetaiotaomicron (B. theta), a prominent gut symbiont of the phylum Bacteroidetes.
30042191	2	16	theme	gut	427:429	arg1	symbiont					431:438	a prominent gut symbiont	415:438	a prominent gut symbiont of the phylum Bacteroidetes	415:466	We found that a heavily glycosylated monoclonal IgA recognizing ovalbumin coats Bacteroides thetaiotaomicron (B. theta), a prominent gut symbiont of the phylum Bacteroidetes.
30042191	2	16	theme	gut	427:429	arg1	thetaiotaomicron					386:401	Bacteroides thetaiotaomicron	374:401	Bacteroides thetaiotaomicron (B. theta)	374:412	We found that a heavily glycosylated monoclonal IgA recognizing ovalbumin coats Bacteroides thetaiotaomicron (B. theta), a prominent gut symbiont of the phylum Bacteroidetes.
30042191	5	17	theme	induced	953:959	arg1	model					961:965	a chemically induced model	940:965	a chemically induced model of colitis	940:976	Expression of the MAFF system facilitates symbiosis with other members of the phylum Firmicutes and promotes protection from a chemically induced model of colitis.
30042191	3	18	theme	uncharacterized	571:585	arg1	family					597:602	a functionally uncharacterized molecular family	556:602	a functionally uncharacterized molecular family provisionally named Mucus-Associated Functional Factor (MAFF)	556:664	In vivo, IgA alters the expression of polysaccharide utilization loci (PUL), including a functionally uncharacterized molecular family provisionally named Mucus-Associated Functional Factor (MAFF).
30042191	6	19	theme	colonic	1049:1055	arg1	homeostasis					1057:1067	colonic homeostasis	1049:1067	colonic homeostasis	1049:1067	Our data reveal a novel mechanism by which IgA promotes symbiosis and colonic homeostasis.
30042191	2	20	theme	prominent	417:425	arg1	symbiont					431:438	a prominent gut symbiont	415:438	a prominent gut symbiont of the phylum Bacteroidetes	415:466	We found that a heavily glycosylated monoclonal IgA recognizing ovalbumin coats Bacteroides thetaiotaomicron (B. theta), a prominent gut symbiont of the phylum Bacteroidetes.
30042191	2	20	theme	prominent	417:425	arg1	thetaiotaomicron					386:401	Bacteroides thetaiotaomicron	374:401	Bacteroides thetaiotaomicron (B. theta)	374:412	We found that a heavily glycosylated monoclonal IgA recognizing ovalbumin coats Bacteroides thetaiotaomicron (B. theta), a prominent gut symbiont of the phylum Bacteroidetes.
30042191	5	21	theme	MAFF	833:836	arg1	system					838:843	the MAFF system	829:843	the MAFF system	829:843	Expression of the MAFF system facilitates symbiosis with other members of the phylum Firmicutes and promotes protection from a chemically induced model of colitis.
30042191	0	22	theme	metabolic	34:42	arg1	function					44:51	metabolic function	34:51	metabolic function	34:51	IgA regulates the composition and metabolic function of gut microbiota by promoting symbiosis between bacteria.
30042191	5	23	theme	system	838:843	arg1	Expression					815:824	Expression	815:824	Expression of the MAFF system	815:843	Expression of the MAFF system facilitates symbiosis with other members of the phylum Firmicutes and promotes protection from a chemically induced model of colitis.
30042191	1	24	dep	composition	169:179	arg1	the					165:167	the	165:167	the	165:167	Immunoglobulin A (IgA) promotes health by regulating the composition and function of gut microbiota, but the molecular requirements for such homeostatic IgA function remain unknown.
30042191	1	25	theme	gut	197:199	arg1	microbiota					201:210	gut microbiota	197:210	gut microbiota	197:210	Immunoglobulin A (IgA) promotes health by regulating the composition and function of gut microbiota, but the molecular requirements for such homeostatic IgA function remain unknown.
30042191	3	26	theme	Functional	641:650	arg1	MAFF					660:663	MAFF	660:663	MAFF	660:663	In vivo, IgA alters the expression of polysaccharide utilization loci (PUL), including a functionally uncharacterized molecular family provisionally named Mucus-Associated Functional Factor (MAFF).
30042191	3	26	theme	Functional	641:650	arg1	Factor					652:657	Mucus-Associated Functional Factor	624:657	Mucus-Associated Functional Factor (MAFF)	624:664	In vivo, IgA alters the expression of polysaccharide utilization loci (PUL), including a functionally uncharacterized molecular family provisionally named Mucus-Associated Functional Factor (MAFF).
30042191	3	27	theme	molecular	587:595	arg1	family					597:602	a functionally uncharacterized molecular family	556:602	a functionally uncharacterized molecular family provisionally named Mucus-Associated Functional Factor (MAFF)	556:664	In vivo, IgA alters the expression of polysaccharide utilization loci (PUL), including a functionally uncharacterized molecular family provisionally named Mucus-Associated Functional Factor (MAFF).
30042191	1	28	theme	microbiota	201:210	arg1	composition					169:179	composition	169:179	composition	169:179	Immunoglobulin A (IgA) promotes health by regulating the composition and function of gut microbiota, but the molecular requirements for such homeostatic IgA function remain unknown.
30042191	1	28	theme	microbiota	201:210	arg1	function					185:192	function	185:192	function	185:192	Immunoglobulin A (IgA) promotes health by regulating the composition and function of gut microbiota, but the molecular requirements for such homeostatic IgA function remain unknown.
30042191	2	29	theme	phylum	447:452	arg1	symbiont					431:438	a prominent gut symbiont	415:438	a prominent gut symbiont of the phylum Bacteroidetes	415:466	We found that a heavily glycosylated monoclonal IgA recognizing ovalbumin coats Bacteroides thetaiotaomicron (B. theta), a prominent gut symbiont of the phylum Bacteroidetes.
30042191	2	29	theme	phylum	447:452	arg1	thetaiotaomicron					386:401	Bacteroides thetaiotaomicron	374:401	Bacteroides thetaiotaomicron (B. theta)	374:412	We found that a heavily glycosylated monoclonal IgA recognizing ovalbumin coats Bacteroides thetaiotaomicron (B. theta), a prominent gut symbiont of the phylum Bacteroidetes.
30042191	2	30	gly	glycosylated	318:329	arg1	IgA					342:344	a heavily glycosylated monoclonal IgA	308:344	a heavily glycosylated monoclonal IgA recognizing ovalbumin	308:366	We found that a heavily glycosylated monoclonal IgA recognizing ovalbumin coats Bacteroides thetaiotaomicron (B. theta), a prominent gut symbiont of the phylum Bacteroidetes.
30042191	1	31	theme	Immunoglobulin	112:125	arg1	IgA					130:132	IgA	130:132	IgA	130:132	Immunoglobulin A (IgA) promotes health by regulating the composition and function of gut microbiota, but the molecular requirements for such homeostatic IgA function remain unknown.
30042191	1	31	theme	Immunoglobulin	112:125	arg1	A					127:127	Immunoglobulin A	112:127	Immunoglobulin A (IgA)	112:133	Immunoglobulin A (IgA) promotes health by regulating the composition and function of gut microbiota, but the molecular requirements for such homeostatic IgA function remain unknown.
30042191	2	32	theme	glycosylated	318:329	arg1	IgA					342:344	a heavily glycosylated monoclonal IgA	308:344	a heavily glycosylated monoclonal IgA recognizing ovalbumin	308:366	We found that a heavily glycosylated monoclonal IgA recognizing ovalbumin coats Bacteroides thetaiotaomicron (B. theta), a prominent gut symbiont of the phylum Bacteroidetes.
30042191	5	33	with	symbiosis	857:865	arg1	members					878:884	other members	872:884	other members of the phylum Firmicutes	872:909	Expression of the MAFF system facilitates symbiosis with other members of the phylum Firmicutes and promotes protection from a chemically induced model of colitis.
30042191	0	34	dep	composition	18:28	arg1	the					14:16	the	14:16	the	14:16	IgA regulates the composition and metabolic function of gut microbiota by promoting symbiosis between bacteria.
30042191	4	35	theme	microbiota	803:812	arg1	presence					783:790	the presence	779:790	the presence of complex microbiota	779:812	In both mice and humans, MAFF is detected predominantly in mucus-resident bacteria, and its expression requires the presence of complex microbiota.
30042191	5	36	from	model	961:965	arg1	protection					924:933	protection	924:933	protection from a chemically induced model of colitis	924:976	Expression of the MAFF system facilitates symbiosis with other members of the phylum Firmicutes and promotes protection from a chemically induced model of colitis.
30042191	3	37	theme	Mucus-Associated	624:639	arg1	MAFF					660:663	MAFF	660:663	MAFF	660:663	In vivo, IgA alters the expression of polysaccharide utilization loci (PUL), including a functionally uncharacterized molecular family provisionally named Mucus-Associated Functional Factor (MAFF).
30042191	3	37	theme	Mucus-Associated	624:639	arg1	Factor					652:657	Mucus-Associated Functional Factor	624:657	Mucus-Associated Functional Factor (MAFF)	624:664	In vivo, IgA alters the expression of polysaccharide utilization loci (PUL), including a functionally uncharacterized molecular family provisionally named Mucus-Associated Functional Factor (MAFF).
30042191	0	38	theme	microbiota	60:69	arg1	composition					18:28	composition	18:28	composition	18:28	IgA regulates the composition and metabolic function of gut microbiota by promoting symbiosis between bacteria.
30042191	0	38	theme	microbiota	60:69	arg1	function					44:51	metabolic function	34:51	metabolic function	34:51	IgA regulates the composition and metabolic function of gut microbiota by promoting symbiosis between bacteria.
30042191	4	39	theme	complex	795:801	arg1	microbiota					803:812	complex microbiota	795:812	complex microbiota	795:812	In both mice and humans, MAFF is detected predominantly in mucus-resident bacteria, and its expression requires the presence of complex microbiota.
30042191	0	40	theme	gut	56:58	arg1	microbiota					60:69	gut microbiota	56:69	gut microbiota	56:69	IgA regulates the composition and metabolic function of gut microbiota by promoting symbiosis between bacteria.
30042191	1	41	theme	molecular	221:229	arg1	requirements					231:242	the molecular requirements	217:242	the molecular requirements for such homeostatic IgA function	217:276	Immunoglobulin A (IgA) promotes health by regulating the composition and function of gut microbiota, but the molecular requirements for such homeostatic IgA function remain unknown.
31257352	3	0	dep	METHODS	654:660	arg1	completed					693:701	completed	693:701	completed 4-day	693:707	METHODS A total of 92 women and 92 men completed 4-day weighed dietary records (DRs) besides the DHQ and BDHQ in each of the four seasons.
31257352	2	1	theme	brief	468:472	arg1	BDHQ					479:482	BDHQ	479:482	BDHQ	479:482	We examined the relative validity of two widely-used dietary assessment questionnaires for Japanese adults, the comprehensive Diet History Questionnaire (DHQ) and the brief DHQ (BDHQ), for estimating the intake of starch and 10 types of sugars: total sugar, sucrose, maltose, lactose, trehalose, glucose, fructose, galactose, and added and free sugars.
31257352	2	1	theme	brief	468:472	arg1	DHQ					474:476	the brief DHQ	464:476	the brief DHQ (BDHQ)	464:483	We examined the relative validity of two widely-used dietary assessment questionnaires for Japanese adults, the comprehensive Diet History Questionnaire (DHQ) and the brief DHQ (BDHQ), for estimating the intake of starch and 10 types of sugars: total sugar, sucrose, maltose, lactose, trehalose, glucose, fructose, galactose, and added and free sugars.
31257352	2	1	theme	brief	468:472	arg1	questionnaires					373:386	two widely-used dietary assessment questionnaires	338:386	two widely-used dietary assessment questionnaires for Japanese adults	338:406	We examined the relative validity of two widely-used dietary assessment questionnaires for Japanese adults, the comprehensive Diet History Questionnaire (DHQ) and the brief DHQ (BDHQ), for estimating the intake of starch and 10 types of sugars: total sugar, sucrose, maltose, lactose, trehalose, glucose, fructose, galactose, and added and free sugars.
31257352	0	2	from	Validity	9:16	arg1	Adults					57:62	Japanese Adults	48:62	Japanese Adults as Estimated With Comprehensive and Brief Self-Administered Diet History Questionnaires	48:150	Relative Validity of Starch and Sugar Intake in Japanese Adults as Estimated With Comprehensive and Brief Self-Administered Diet History Questionnaires.
31257352	2	3	theme	total	546:550	arg1	sugar					552:556	total sugar	546:556	total sugar	546:556	We examined the relative validity of two widely-used dietary assessment questionnaires for Japanese adults, the comprehensive Diet History Questionnaire (DHQ) and the brief DHQ (BDHQ), for estimating the intake of starch and 10 types of sugars: total sugar, sucrose, maltose, lactose, trehalose, glucose, fructose, galactose, and added and free sugars.
31257352	6	4	theme	Spearman	1190:1197	arg1	coefficients					1211:1222	Spearman correlation coefficients	1190:1222	Spearman correlation coefficients between the 16-day DRs and DHQ1	1190:1254	Spearman correlation coefficients between the 16-day DRs and DHQ1 were acceptable (≥0.31) for all variables (0.31-0.67), except for maltose and trehalose in women (≤0.29).
31257352	9	5	dep	CONCLUSIONS	1559:1569	arg1	indicated					1582:1590	indicated	1582:1590	indicated	1582:1590	CONCLUSIONS This study indicated a reasonable ranking ability of DHQ and BDHQ for the intake of starch and most sugars examined, despite a poor ability to estimate the intake at the both group and individual levels.
31257352	5	6	theme	first	1063:1067	arg1	DHQ					1069:1071	the first DHQ	1059:1071	the first DHQ	1059:1071	RESULTS For most of the carbohydrate variables examined, the median energy-adjusted intake derived from the first DHQ and BDHQ (DHQ1 and BDHQ1, respectively) significantly differed from those derived from the 16-day DRs in both sexes.
31257352	0	7	theme	Comprehensive	82:94	arg1	Questionnaires					137:150	Comprehensive and Brief Self-Administered Diet History Questionnaires	82:150	Comprehensive and Brief Self-Administered Diet History Questionnaires	82:150	Relative Validity of Starch and Sugar Intake in Japanese Adults as Estimated With Comprehensive and Brief Self-Administered Diet History Questionnaires.
31257352	3	8	theme	women	676:680	arg1	total					664:668	A total	662:668	A total of 92 women and 92 men	662:691	METHODS A total of 92 women and 92 men completed 4-day weighed dietary records (DRs) besides the DHQ and BDHQ in each of the four seasons.
31257352	8	9	theme	DHQs	1543:1546	arg1	mean					1530:1533	the mean	1526:1533	the mean of four DHQs and BDHQs	1526:1556	Similar results were observed for the mean of four DHQs and BDHQs.
31257352	6	10	theme	correlation	1199:1209	arg1	coefficients					1211:1222	Spearman correlation coefficients	1190:1222	Spearman correlation coefficients between the 16-day DRs and DHQ1	1190:1254	Spearman correlation coefficients between the 16-day DRs and DHQ1 were acceptable (≥0.31) for all variables (0.31-0.67), except for maltose and trehalose in women (≤0.29).
31257352	2	11	theme	sugars	538:543	arg1	types					529:533	10 types	526:533	10 types of sugars: total sugar, sucrose, maltose, lactose, trehalose, glucose, fructose, galactose	526:624	We examined the relative validity of two widely-used dietary assessment questionnaires for Japanese adults, the comprehensive Diet History Questionnaire (DHQ) and the brief DHQ (BDHQ), for estimating the intake of starch and 10 types of sugars: total sugar, sucrose, maltose, lactose, trehalose, glucose, fructose, galactose, and added and free sugars.
31257352	2	11	theme	sugars	538:543	arg1	intake					505:510	the intake	501:510	the intake of starch	501:520	We examined the relative validity of two widely-used dietary assessment questionnaires for Japanese adults, the comprehensive Diet History Questionnaire (DHQ) and the brief DHQ (BDHQ), for estimating the intake of starch and 10 types of sugars: total sugar, sucrose, maltose, lactose, trehalose, glucose, fructose, galactose, and added and free sugars.
31257352	3	12	theme	dietary	717:723	arg1	DRs					734:736	DRs	734:736	DRs	734:736	METHODS A total of 92 women and 92 men completed 4-day weighed dietary records (DRs) besides the DHQ and BDHQ in each of the four seasons.
31257352	3	12	theme	dietary	717:723	arg1	records					725:731	dietary records	717:731	dietary records (DRs)	717:737	METHODS A total of 92 women and 92 men completed 4-day weighed dietary records (DRs) besides the DHQ and BDHQ in each of the four seasons.
31257352	0	13	theme	Brief	100:104	arg1	Questionnaires					137:150	Comprehensive and Brief Self-Administered Diet History Questionnaires	82:150	Comprehensive and Brief Self-Administered Diet History Questionnaires	82:150	Relative Validity of Starch and Sugar Intake in Japanese Adults as Estimated With Comprehensive and Brief Self-Administered Diet History Questionnaires.
31257352	1	14	from	BACKGROUND	153:162	arg1	Japan					167:171	Japan	167:171	Japan	167:171	BACKGROUND In Japan, large-scale epidemiological studies on starch and sugar intake are scarce, mainly due to a lack of a suitable assessment tool.
31257352	2	15	theme	questionnaires	373:386	arg1	validity					326:333	the relative validity	313:333	the relative validity of two widely-used dietary assessment questionnaires for Japanese adults, the comprehensive Diet History Questionnaire (DHQ) and the brief DHQ (BDHQ), for estimating the intake of starch and 10 types of sugars: total sugar, sucrose, maltose, lactose, trehalose, glucose, fructose, galactose, and added and free sugars	313:651	We examined the relative validity of two widely-used dietary assessment questionnaires for Japanese adults, the comprehensive Diet History Questionnaire (DHQ) and the brief DHQ (BDHQ), for estimating the intake of starch and 10 types of sugars: total sugar, sucrose, maltose, lactose, trehalose, glucose, fructose, galactose, and added and free sugars.
31257352	2	16	theme	Japanese	392:399	arg1	adults					401:406	Japanese adults	392:406	Japanese adults	392:406	We examined the relative validity of two widely-used dietary assessment questionnaires for Japanese adults, the comprehensive Diet History Questionnaire (DHQ) and the brief DHQ (BDHQ), for estimating the intake of starch and 10 types of sugars: total sugar, sucrose, maltose, lactose, trehalose, glucose, fructose, galactose, and added and free sugars.
31257352	9	17	theme	ranking	1605:1611	arg1	ability					1613:1619	a reasonable ranking ability	1592:1619	a reasonable ranking ability of DHQ and BDHQ for the intake of starch and most sugars examined	1592:1685	CONCLUSIONS This study indicated a reasonable ranking ability of DHQ and BDHQ for the intake of starch and most sugars examined, despite a poor ability to estimate the intake at the both group and individual levels.
31257352	3	18	dep	DHQ	751:753	arg1	the					747:749	the	747:749	the	747:749	METHODS A total of 92 women and 92 men completed 4-day weighed dietary records (DRs) besides the DHQ and BDHQ in each of the four seasons.
31257352	4	19	theme	developed	873:881	arg1	database					900:907	a recently developed food composition database	862:907	a recently developed food composition database on starch and sugars for Japanese food items	862:952	For each method, starch and sugar intake was calculated according to a recently developed food composition database on starch and sugars for Japanese food items.
31257352	1	20	theme	large-scale	174:184	arg1	BACKGROUND					153:162	BACKGROUND	153:162	BACKGROUND In Japan	153:171	BACKGROUND In Japan, large-scale epidemiological studies on starch and sugar intake are scarce, mainly due to a lack of a suitable assessment tool.
31257352	1	20	theme	large-scale	174:184	arg1	studies					202:208	large-scale epidemiological studies	174:208	large-scale epidemiological studies on starch and sugar intake	174:235	BACKGROUND In Japan, large-scale epidemiological studies on starch and sugar intake are scarce, mainly due to a lack of a suitable assessment tool.
31257352	4	21	theme	food	883:886	arg1	database					900:907	a recently developed food composition database	862:907	a recently developed food composition database on starch and sugars for Japanese food items	862:952	For each method, starch and sugar intake was calculated according to a recently developed food composition database on starch and sugars for Japanese food items.
31257352	6	22	theme	16-day	1236:1241	arg1	DRs					1243:1245	the 16-day DRs	1232:1245	the 16-day DRs	1232:1245	Spearman correlation coefficients between the 16-day DRs and DHQ1 were acceptable (≥0.31) for all variables (0.31-0.67), except for maltose and trehalose in women (≤0.29).
31257352	5	23	theme	energy-adjusted	1023:1037	arg1	intake					1039:1044	the median energy-adjusted intake	1012:1044	the median energy-adjusted intake derived from the first DHQ	1012:1071	RESULTS For most of the carbohydrate variables examined, the median energy-adjusted intake derived from the first DHQ and BDHQ (DHQ1 and BDHQ1, respectively) significantly differed from those derived from the 16-day DRs in both sexes.
31257352	5	24	attach	derived	1147:1153	arg2	those					1141:1145	those	1141:1145	those	1141:1145	RESULTS For most of the carbohydrate variables examined, the median energy-adjusted intake derived from the first DHQ and BDHQ (DHQ1 and BDHQ1, respectively) significantly differed from those derived from the 16-day DRs in both sexes.
31257352	5	24	attach	derived	1147:1153	arg1	DRs					1171:1173	the 16-day DRs	1160:1173	the 16-day DRs in both sexes	1160:1187	RESULTS For most of the carbohydrate variables examined, the median energy-adjusted intake derived from the first DHQ and BDHQ (DHQ1 and BDHQ1, respectively) significantly differed from those derived from the 16-day DRs in both sexes.
31257352	1	25	theme	epidemiological	186:200	arg1	BACKGROUND					153:162	BACKGROUND	153:162	BACKGROUND In Japan	153:171	BACKGROUND In Japan, large-scale epidemiological studies on starch and sugar intake are scarce, mainly due to a lack of a suitable assessment tool.
31257352	1	25	theme	epidemiological	186:200	arg1	studies					202:208	large-scale epidemiological studies	174:208	large-scale epidemiological studies on starch and sugar intake	174:235	BACKGROUND In Japan, large-scale epidemiological studies on starch and sugar intake are scarce, mainly due to a lack of a suitable assessment tool.
31257352	0	26	theme	Relative	0:7	arg1	Validity					9:16	Relative Validity	0:16	Relative Validity of Starch and Sugar Intake in Japanese Adults as Estimated With Comprehensive and Brief Self-Administered Diet History Questionnaires.	0:151	Relative Validity of Starch and Sugar Intake in Japanese Adults as Estimated With Comprehensive and Brief Self-Administered Diet History Questionnaires.
31257352	4	27	from	database	900:907	arg1	sugars					923:928	sugars	923:928	sugars	923:928	For each method, starch and sugar intake was calculated according to a recently developed food composition database on starch and sugars for Japanese food items.
31257352	4	27	from	database	900:907	arg1	starch					912:917	starch	912:917	starch	912:917	For each method, starch and sugar intake was calculated according to a recently developed food composition database on starch and sugars for Japanese food items.
31257352	6	28	from	maltose	1322:1328	arg1	≤0.29					1354:1358	≤0.29	1354:1358	≤0.29	1354:1358	Spearman correlation coefficients between the 16-day DRs and DHQ1 were acceptable (≥0.31) for all variables (0.31-0.67), except for maltose and trehalose in women (≤0.29).
31257352	6	28	from	maltose	1322:1328	arg1	women					1347:1351	women	1347:1351	women (≤0.29)	1347:1359	Spearman correlation coefficients between the 16-day DRs and DHQ1 were acceptable (≥0.31) for all variables (0.31-0.67), except for maltose and trehalose in women (≤0.29).
31257352	9	29	theme	sugars	1671:1676	arg1	intake					1645:1650	the intake	1641:1650	the intake of starch and most sugars examined	1641:1685	CONCLUSIONS This study indicated a reasonable ranking ability of DHQ and BDHQ for the intake of starch and most sugars examined, despite a poor ability to estimate the intake at the both group and individual levels.
31257352	2	30	theme	assessment	362:371	arg1	Questionnaire					440:452	the comprehensive Diet History Questionnaire	409:452	the comprehensive Diet History Questionnaire (DHQ)	409:458	We examined the relative validity of two widely-used dietary assessment questionnaires for Japanese adults, the comprehensive Diet History Questionnaire (DHQ) and the brief DHQ (BDHQ), for estimating the intake of starch and 10 types of sugars: total sugar, sucrose, maltose, lactose, trehalose, glucose, fructose, galactose, and added and free sugars.
31257352	2	30	theme	assessment	362:371	arg1	questionnaires					373:386	two widely-used dietary assessment questionnaires	338:386	two widely-used dietary assessment questionnaires for Japanese adults	338:406	We examined the relative validity of two widely-used dietary assessment questionnaires for Japanese adults, the comprehensive Diet History Questionnaire (DHQ) and the brief DHQ (BDHQ), for estimating the intake of starch and 10 types of sugars: total sugar, sucrose, maltose, lactose, trehalose, glucose, fructose, galactose, and added and free sugars.
31257352	2	30	theme	assessment	362:371	arg1	DHQ					474:476	the brief DHQ	464:476	the brief DHQ (BDHQ)	464:483	We examined the relative validity of two widely-used dietary assessment questionnaires for Japanese adults, the comprehensive Diet History Questionnaire (DHQ) and the brief DHQ (BDHQ), for estimating the intake of starch and 10 types of sugars: total sugar, sucrose, maltose, lactose, trehalose, glucose, fructose, galactose, and added and free sugars.
31257352	0	31	theme	Starch	21:26	arg1	Validity					9:16	Relative Validity	0:16	Relative Validity of Starch and Sugar Intake in Japanese Adults as Estimated With Comprehensive and Brief Self-Administered Diet History Questionnaires.	0:151	Relative Validity of Starch and Sugar Intake in Japanese Adults as Estimated With Comprehensive and Brief Self-Administered Diet History Questionnaires.
31257352	9	32	theme	most	1666:1669	arg1	sugars					1671:1676	most sugars	1666:1676	most sugars examined	1666:1685	CONCLUSIONS This study indicated a reasonable ranking ability of DHQ and BDHQ for the intake of starch and most sugars examined, despite a poor ability to estimate the intake at the both group and individual levels.
31257352	0	33	theme	Diet	124:127	arg1	Questionnaires					137:150	Comprehensive and Brief Self-Administered Diet History Questionnaires	82:150	Comprehensive and Brief Self-Administered Diet History Questionnaires	82:150	Relative Validity of Starch and Sugar Intake in Japanese Adults as Estimated With Comprehensive and Brief Self-Administered Diet History Questionnaires.
31257352	0	34	dep	Adults	57:62	arg1	Estimated					67:75	Estimated	67:75	Estimated With Comprehensive and Brief Self-Administered Diet History Questionnaires	67:150	Relative Validity of Starch and Sugar Intake in Japanese Adults as Estimated With Comprehensive and Brief Self-Administered Diet History Questionnaires.
31257352	8	35	theme	Similar	1492:1498	arg1	results					1500:1506	Similar results	1492:1506	Similar results	1492:1506	Similar results were observed for the mean of four DHQs and BDHQs.
31257352	2	36	theme	comprehensive	413:425	arg1	Questionnaire					440:452	the comprehensive Diet History Questionnaire	409:452	the comprehensive Diet History Questionnaire (DHQ)	409:458	We examined the relative validity of two widely-used dietary assessment questionnaires for Japanese adults, the comprehensive Diet History Questionnaire (DHQ) and the brief DHQ (BDHQ), for estimating the intake of starch and 10 types of sugars: total sugar, sucrose, maltose, lactose, trehalose, glucose, fructose, galactose, and added and free sugars.
31257352	2	36	theme	comprehensive	413:425	arg1	questionnaires					373:386	two widely-used dietary assessment questionnaires	338:386	two widely-used dietary assessment questionnaires for Japanese adults	338:406	We examined the relative validity of two widely-used dietary assessment questionnaires for Japanese adults, the comprehensive Diet History Questionnaire (DHQ) and the brief DHQ (BDHQ), for estimating the intake of starch and 10 types of sugars: total sugar, sucrose, maltose, lactose, trehalose, glucose, fructose, galactose, and added and free sugars.
31257352	2	36	theme	comprehensive	413:425	arg1	DHQ					455:457	DHQ	455:457	DHQ	455:457	We examined the relative validity of two widely-used dietary assessment questionnaires for Japanese adults, the comprehensive Diet History Questionnaire (DHQ) and the brief DHQ (BDHQ), for estimating the intake of starch and 10 types of sugars: total sugar, sucrose, maltose, lactose, trehalose, glucose, fructose, galactose, and added and free sugars.
31257352	2	37	theme	Diet	427:430	arg1	Questionnaire					440:452	the comprehensive Diet History Questionnaire	409:452	the comprehensive Diet History Questionnaire (DHQ)	409:458	We examined the relative validity of two widely-used dietary assessment questionnaires for Japanese adults, the comprehensive Diet History Questionnaire (DHQ) and the brief DHQ (BDHQ), for estimating the intake of starch and 10 types of sugars: total sugar, sucrose, maltose, lactose, trehalose, glucose, fructose, galactose, and added and free sugars.
31257352	2	37	theme	Diet	427:430	arg1	questionnaires					373:386	two widely-used dietary assessment questionnaires	338:386	two widely-used dietary assessment questionnaires for Japanese adults	338:406	We examined the relative validity of two widely-used dietary assessment questionnaires for Japanese adults, the comprehensive Diet History Questionnaire (DHQ) and the brief DHQ (BDHQ), for estimating the intake of starch and 10 types of sugars: total sugar, sucrose, maltose, lactose, trehalose, glucose, fructose, galactose, and added and free sugars.
31257352	2	37	theme	Diet	427:430	arg1	DHQ					455:457	DHQ	455:457	DHQ	455:457	We examined the relative validity of two widely-used dietary assessment questionnaires for Japanese adults, the comprehensive Diet History Questionnaire (DHQ) and the brief DHQ (BDHQ), for estimating the intake of starch and 10 types of sugars: total sugar, sucrose, maltose, lactose, trehalose, glucose, fructose, galactose, and added and free sugars.
31257352	0	38	theme	Self-Administered	106:122	arg1	Questionnaires					137:150	Comprehensive and Brief Self-Administered Diet History Questionnaires	82:150	Comprehensive and Brief Self-Administered Diet History Questionnaires	82:150	Relative Validity of Starch and Sugar Intake in Japanese Adults as Estimated With Comprehensive and Brief Self-Administered Diet History Questionnaires.
31257352	6	39	from	trehalose	1334:1342	arg1	≤0.29					1354:1358	≤0.29	1354:1358	≤0.29	1354:1358	Spearman correlation coefficients between the 16-day DRs and DHQ1 were acceptable (≥0.31) for all variables (0.31-0.67), except for maltose and trehalose in women (≤0.29).
31257352	6	39	from	trehalose	1334:1342	arg1	women					1347:1351	women	1347:1351	women (≤0.29)	1347:1359	Spearman correlation coefficients between the 16-day DRs and DHQ1 were acceptable (≥0.31) for all variables (0.31-0.67), except for maltose and trehalose in women (≤0.29).
31257352	9	40	theme	starch	1655:1660	arg1	intake					1645:1650	the intake	1641:1650	the intake of starch and most sugars examined	1641:1685	CONCLUSIONS This study indicated a reasonable ranking ability of DHQ and BDHQ for the intake of starch and most sugars examined, despite a poor ability to estimate the intake at the both group and individual levels.
31257352	0	41	theme	Sugar	32:36	arg1	Intake					38:43	Sugar Intake	32:43	Sugar Intake	32:43	Relative Validity of Starch and Sugar Intake in Japanese Adults as Estimated With Comprehensive and Brief Self-Administered Diet History Questionnaires.
31257352	1	42	theme	suitable	275:282	arg1	tool					295:298	a suitable assessment tool	273:298	a suitable assessment tool	273:298	BACKGROUND In Japan, large-scale epidemiological studies on starch and sugar intake are scarce, mainly due to a lack of a suitable assessment tool.
31257352	5	43	theme	16-day	1164:1169	arg1	DRs					1171:1173	the 16-day DRs	1160:1173	the 16-day DRs in both sexes	1160:1187	RESULTS For most of the carbohydrate variables examined, the median energy-adjusted intake derived from the first DHQ and BDHQ (DHQ1 and BDHQ1, respectively) significantly differed from those derived from the 16-day DRs in both sexes.
31257352	2	44	dep	sugars	538:543	arg1	sugar					552:556	total sugar	546:556	total sugar	546:556	We examined the relative validity of two widely-used dietary assessment questionnaires for Japanese adults, the comprehensive Diet History Questionnaire (DHQ) and the brief DHQ (BDHQ), for estimating the intake of starch and 10 types of sugars: total sugar, sucrose, maltose, lactose, trehalose, glucose, fructose, galactose, and added and free sugars.
31257352	1	45	theme	assessment	284:293	arg1	tool					295:298	a suitable assessment tool	273:298	a suitable assessment tool	273:298	BACKGROUND In Japan, large-scale epidemiological studies on starch and sugar intake are scarce, mainly due to a lack of a suitable assessment tool.
31257352	0	46	theme	History	129:135	arg1	Questionnaires					137:150	Comprehensive and Brief Self-Administered Diet History Questionnaires	82:150	Comprehensive and Brief Self-Administered Diet History Questionnaires	82:150	Relative Validity of Starch and Sugar Intake in Japanese Adults as Estimated With Comprehensive and Brief Self-Administered Diet History Questionnaires.
31257352	5	47	dep	BDHQ	1077:1080	arg1	BDHQ1					1092:1096	BDHQ1	1092:1096	BDHQ1	1092:1096	RESULTS For most of the carbohydrate variables examined, the median energy-adjusted intake derived from the first DHQ and BDHQ (DHQ1 and BDHQ1, respectively) significantly differed from those derived from the 16-day DRs in both sexes.
31257352	5	47	dep	BDHQ	1077:1080	arg1	DHQ1					1083:1086	DHQ1	1083:1086	DHQ1	1083:1086	RESULTS For most of the carbohydrate variables examined, the median energy-adjusted intake derived from the first DHQ and BDHQ (DHQ1 and BDHQ1, respectively) significantly differed from those derived from the 16-day DRs in both sexes.
31257352	5	47	dep	BDHQ	1077:1080	arg1	BDHQ					1077:1080	BDHQ	1077:1080	BDHQ (DHQ1 and BDHQ1, respectively)	1077:1111	RESULTS For most of the carbohydrate variables examined, the median energy-adjusted intake derived from the first DHQ and BDHQ (DHQ1 and BDHQ1, respectively) significantly differed from those derived from the 16-day DRs in both sexes.
31257352	2	48	theme	History	432:438	arg1	Questionnaire					440:452	the comprehensive Diet History Questionnaire	409:452	the comprehensive Diet History Questionnaire (DHQ)	409:458	We examined the relative validity of two widely-used dietary assessment questionnaires for Japanese adults, the comprehensive Diet History Questionnaire (DHQ) and the brief DHQ (BDHQ), for estimating the intake of starch and 10 types of sugars: total sugar, sucrose, maltose, lactose, trehalose, glucose, fructose, galactose, and added and free sugars.
31257352	2	48	theme	History	432:438	arg1	questionnaires					373:386	two widely-used dietary assessment questionnaires	338:386	two widely-used dietary assessment questionnaires for Japanese adults	338:406	We examined the relative validity of two widely-used dietary assessment questionnaires for Japanese adults, the comprehensive Diet History Questionnaire (DHQ) and the brief DHQ (BDHQ), for estimating the intake of starch and 10 types of sugars: total sugar, sucrose, maltose, lactose, trehalose, glucose, fructose, galactose, and added and free sugars.
31257352	2	48	theme	History	432:438	arg1	DHQ					455:457	DHQ	455:457	DHQ	455:457	We examined the relative validity of two widely-used dietary assessment questionnaires for Japanese adults, the comprehensive Diet History Questionnaire (DHQ) and the brief DHQ (BDHQ), for estimating the intake of starch and 10 types of sugars: total sugar, sucrose, maltose, lactose, trehalose, glucose, fructose, galactose, and added and free sugars.
31257352	1	49	theme	sugar	224:228	arg1	intake					230:235	sugar intake	224:235	sugar intake	224:235	BACKGROUND In Japan, large-scale epidemiological studies on starch and sugar intake are scarce, mainly due to a lack of a suitable assessment tool.
31257352	9	50	theme	reasonable	1594:1603	arg1	ability					1613:1619	a reasonable ranking ability	1592:1619	a reasonable ranking ability of DHQ and BDHQ for the intake of starch and most sugars examined	1592:1685	CONCLUSIONS This study indicated a reasonable ranking ability of DHQ and BDHQ for the intake of starch and most sugars examined, despite a poor ability to estimate the intake at the both group and individual levels.
31257352	1	51	theme	tool	295:298	arg1	lack					265:268	a lack	263:268	a lack of a suitable assessment tool	263:298	BACKGROUND In Japan, large-scale epidemiological studies on starch and sugar intake are scarce, mainly due to a lack of a suitable assessment tool.
31257352	4	52	theme	sugar	821:825	arg1	intake					827:832	sugar intake	821:832	sugar intake	821:832	For each method, starch and sugar intake was calculated according to a recently developed food composition database on starch and sugars for Japanese food items.
31257352	9	53	theme	poor	1698:1701	arg1	ability					1703:1709	a poor ability	1696:1709	a poor ability to estimate the intake at the both group and individual levels	1696:1772	CONCLUSIONS This study indicated a reasonable ranking ability of DHQ and BDHQ for the intake of starch and most sugars examined, despite a poor ability to estimate the intake at the both group and individual levels.
31257352	5	54	theme	carbohydrate	979:990	arg1	variables					992:1000	the carbohydrate variables	975:1000	the carbohydrate variables examined	975:1009	RESULTS For most of the carbohydrate variables examined, the median energy-adjusted intake derived from the first DHQ and BDHQ (DHQ1 and BDHQ1, respectively) significantly differed from those derived from the 16-day DRs in both sexes.
31257352	0	55	theme	Intake	38:43	arg1	Validity					9:16	Relative Validity	0:16	Relative Validity of Starch and Sugar Intake in Japanese Adults as Estimated With Comprehensive and Brief Self-Administered Diet History Questionnaires.	0:151	Relative Validity of Starch and Sugar Intake in Japanese Adults as Estimated With Comprehensive and Brief Self-Administered Diet History Questionnaires.
31257352	2	56	theme	starch	515:520	arg1	types					529:533	10 types	526:533	10 types of sugars: total sugar, sucrose, maltose, lactose, trehalose, glucose, fructose, galactose	526:624	We examined the relative validity of two widely-used dietary assessment questionnaires for Japanese adults, the comprehensive Diet History Questionnaire (DHQ) and the brief DHQ (BDHQ), for estimating the intake of starch and 10 types of sugars: total sugar, sucrose, maltose, lactose, trehalose, glucose, fructose, galactose, and added and free sugars.
31257352	2	56	theme	starch	515:520	arg1	intake					505:510	the intake	501:510	the intake of starch	501:520	We examined the relative validity of two widely-used dietary assessment questionnaires for Japanese adults, the comprehensive Diet History Questionnaire (DHQ) and the brief DHQ (BDHQ), for estimating the intake of starch and 10 types of sugars: total sugar, sucrose, maltose, lactose, trehalose, glucose, fructose, galactose, and added and free sugars.
31257352	2	57	theme	relative	317:324	arg1	validity					326:333	the relative validity	313:333	the relative validity of two widely-used dietary assessment questionnaires for Japanese adults, the comprehensive Diet History Questionnaire (DHQ) and the brief DHQ (BDHQ), for estimating the intake of starch and 10 types of sugars: total sugar, sucrose, maltose, lactose, trehalose, glucose, fructose, galactose, and added and free sugars	313:651	We examined the relative validity of two widely-used dietary assessment questionnaires for Japanese adults, the comprehensive Diet History Questionnaire (DHQ) and the brief DHQ (BDHQ), for estimating the intake of starch and 10 types of sugars: total sugar, sucrose, maltose, lactose, trehalose, glucose, fructose, galactose, and added and free sugars.
31257352	4	58	theme	Japanese	934:941	arg1	items					948:952	Japanese food items	934:952	Japanese food items	934:952	For each method, starch and sugar intake was calculated according to a recently developed food composition database on starch and sugars for Japanese food items.
31257352	5	59	theme	median	1016:1021	arg1	intake					1039:1044	the median energy-adjusted intake	1012:1044	the median energy-adjusted intake derived from the first DHQ	1012:1071	RESULTS For most of the carbohydrate variables examined, the median energy-adjusted intake derived from the first DHQ and BDHQ (DHQ1 and BDHQ1, respectively) significantly differed from those derived from the 16-day DRs in both sexes.
31257352	9	60	theme	individual	1756:1765	arg1	levels					1767:1772	individual levels	1756:1772	individual levels	1756:1772	CONCLUSIONS This study indicated a reasonable ranking ability of DHQ and BDHQ for the intake of starch and most sugars examined, despite a poor ability to estimate the intake at the both group and individual levels.
31257352	5	61	attach	derived	1046:1052	arg1	DHQ					1069:1071	the first DHQ	1059:1071	the first DHQ	1059:1071	RESULTS For most of the carbohydrate variables examined, the median energy-adjusted intake derived from the first DHQ and BDHQ (DHQ1 and BDHQ1, respectively) significantly differed from those derived from the 16-day DRs in both sexes.
31257352	5	61	attach	derived	1046:1052	arg2	intake					1039:1044	the median energy-adjusted intake	1012:1044	the median energy-adjusted intake derived from the first DHQ	1012:1071	RESULTS For most of the carbohydrate variables examined, the median energy-adjusted intake derived from the first DHQ and BDHQ (DHQ1 and BDHQ1, respectively) significantly differed from those derived from the 16-day DRs in both sexes.
31257352	2	62	theme	sugars	646:651	arg1	validity					326:333	the relative validity	313:333	the relative validity of two widely-used dietary assessment questionnaires for Japanese adults, the comprehensive Diet History Questionnaire (DHQ) and the brief DHQ (BDHQ), for estimating the intake of starch and 10 types of sugars: total sugar, sucrose, maltose, lactose, trehalose, glucose, fructose, galactose, and added and free sugars	313:651	We examined the relative validity of two widely-used dietary assessment questionnaires for Japanese adults, the comprehensive Diet History Questionnaire (DHQ) and the brief DHQ (BDHQ), for estimating the intake of starch and 10 types of sugars: total sugar, sucrose, maltose, lactose, trehalose, glucose, fructose, galactose, and added and free sugars.
31257352	2	63	theme	free	641:644	arg1	sugars					646:651	free sugars	641:651	free sugars	641:651	We examined the relative validity of two widely-used dietary assessment questionnaires for Japanese adults, the comprehensive Diet History Questionnaire (DHQ) and the brief DHQ (BDHQ), for estimating the intake of starch and 10 types of sugars: total sugar, sucrose, maltose, lactose, trehalose, glucose, fructose, galactose, and added and free sugars.
31257352	9	64	theme	DHQ	1624:1626	arg1	ability					1613:1619	a reasonable ranking ability	1592:1619	a reasonable ranking ability of DHQ and BDHQ for the intake of starch and most sugars examined	1592:1685	CONCLUSIONS This study indicated a reasonable ranking ability of DHQ and BDHQ for the intake of starch and most sugars examined, despite a poor ability to estimate the intake at the both group and individual levels.
31257352	5	65	from	DRs	1171:1173	arg1	sexes					1183:1187	both sexes	1178:1187	both sexes	1178:1187	RESULTS For most of the carbohydrate variables examined, the median energy-adjusted intake derived from the first DHQ and BDHQ (DHQ1 and BDHQ1, respectively) significantly differed from those derived from the 16-day DRs in both sexes.
31257352	1	66	from	studies	202:208	arg1	starch					213:218	starch	213:218	starch	213:218	BACKGROUND In Japan, large-scale epidemiological studies on starch and sugar intake are scarce, mainly due to a lack of a suitable assessment tool.
31257352	1	66	from	studies	202:208	arg1	intake					230:235	sugar intake	224:235	sugar intake	224:235	BACKGROUND In Japan, large-scale epidemiological studies on starch and sugar intake are scarce, mainly due to a lack of a suitable assessment tool.
31257352	2	67	theme	dietary	354:360	arg1	Questionnaire					440:452	the comprehensive Diet History Questionnaire	409:452	the comprehensive Diet History Questionnaire (DHQ)	409:458	We examined the relative validity of two widely-used dietary assessment questionnaires for Japanese adults, the comprehensive Diet History Questionnaire (DHQ) and the brief DHQ (BDHQ), for estimating the intake of starch and 10 types of sugars: total sugar, sucrose, maltose, lactose, trehalose, glucose, fructose, galactose, and added and free sugars.
31257352	2	67	theme	dietary	354:360	arg1	questionnaires					373:386	two widely-used dietary assessment questionnaires	338:386	two widely-used dietary assessment questionnaires for Japanese adults	338:406	We examined the relative validity of two widely-used dietary assessment questionnaires for Japanese adults, the comprehensive Diet History Questionnaire (DHQ) and the brief DHQ (BDHQ), for estimating the intake of starch and 10 types of sugars: total sugar, sucrose, maltose, lactose, trehalose, glucose, fructose, galactose, and added and free sugars.
31257352	2	67	theme	dietary	354:360	arg1	DHQ					474:476	the brief DHQ	464:476	the brief DHQ (BDHQ)	464:483	We examined the relative validity of two widely-used dietary assessment questionnaires for Japanese adults, the comprehensive Diet History Questionnaire (DHQ) and the brief DHQ (BDHQ), for estimating the intake of starch and 10 types of sugars: total sugar, sucrose, maltose, lactose, trehalose, glucose, fructose, galactose, and added and free sugars.
31257352	4	68	theme	food	943:946	arg1	items					948:952	Japanese food items	934:952	Japanese food items	934:952	For each method, starch and sugar intake was calculated according to a recently developed food composition database on starch and sugars for Japanese food items.
31257352	0	69	theme	Japanese	48:55	arg1	Adults					57:62	Japanese Adults	48:62	Japanese Adults as Estimated With Comprehensive and Brief Self-Administered Diet History Questionnaires	48:150	Relative Validity of Starch and Sugar Intake in Japanese Adults as Estimated With Comprehensive and Brief Self-Administered Diet History Questionnaires.
31257352	4	70	theme	composition	888:898	arg1	database					900:907	a recently developed food composition database	862:907	a recently developed food composition database on starch and sugars for Japanese food items	862:952	For each method, starch and sugar intake was calculated according to a recently developed food composition database on starch and sugars for Japanese food items.
31257352	8	71	theme	BDHQs	1552:1556	arg1	mean					1530:1533	the mean	1526:1533	the mean of four DHQs and BDHQs	1526:1556	Similar results were observed for the mean of four DHQs and BDHQs.
31257352	2	72	theme	widely-used	342:352	arg1	Questionnaire					440:452	the comprehensive Diet History Questionnaire	409:452	the comprehensive Diet History Questionnaire (DHQ)	409:458	We examined the relative validity of two widely-used dietary assessment questionnaires for Japanese adults, the comprehensive Diet History Questionnaire (DHQ) and the brief DHQ (BDHQ), for estimating the intake of starch and 10 types of sugars: total sugar, sucrose, maltose, lactose, trehalose, glucose, fructose, galactose, and added and free sugars.
31257352	2	72	theme	widely-used	342:352	arg1	questionnaires					373:386	two widely-used dietary assessment questionnaires	338:386	two widely-used dietary assessment questionnaires for Japanese adults	338:406	We examined the relative validity of two widely-used dietary assessment questionnaires for Japanese adults, the comprehensive Diet History Questionnaire (DHQ) and the brief DHQ (BDHQ), for estimating the intake of starch and 10 types of sugars: total sugar, sucrose, maltose, lactose, trehalose, glucose, fructose, galactose, and added and free sugars.
31257352	2	72	theme	widely-used	342:352	arg1	DHQ					474:476	the brief DHQ	464:476	the brief DHQ (BDHQ)	464:483	We examined the relative validity of two widely-used dietary assessment questionnaires for Japanese adults, the comprehensive Diet History Questionnaire (DHQ) and the brief DHQ (BDHQ), for estimating the intake of starch and 10 types of sugars: total sugar, sucrose, maltose, lactose, trehalose, glucose, fructose, galactose, and added and free sugars.
31257352	9	73	theme	BDHQ	1632:1635	arg1	ability					1613:1619	a reasonable ranking ability	1592:1619	a reasonable ranking ability of DHQ and BDHQ for the intake of starch and most sugars examined	1592:1685	CONCLUSIONS This study indicated a reasonable ranking ability of DHQ and BDHQ for the intake of starch and most sugars examined, despite a poor ability to estimate the intake at the both group and individual levels.
31257352	3	74	theme	men	689:691	arg1	total					664:668	A total	662:668	A total of 92 women and 92 men	662:691	METHODS A total of 92 women and 92 men completed 4-day weighed dietary records (DRs) besides the DHQ and BDHQ in each of the four seasons.
29545893	0	0	theme	immunosuppression	119:135	arg1	mice					137:140	cyclophosphamide-induced immunosuppression mice	94:140	cyclophosphamide-induced immunosuppression mice	94:140	The investigation of immunomodulatory activities of Gloeostereum incaratum polysaccharides in cyclophosphamide-induced immunosuppression mice.
29545893	5	1	theme	increased	1144:1152	arg1	levels					1175:1180	the increased superoxide dismutase levels	1140:1180	the increased superoxide dismutase levels in the serum and spleen	1140:1204	Finally, GIPS showed anti-oxidative effects indicated by the increased superoxide dismutase levels in the serum and spleen, and the reduced level of reactive oxygen species in the spleen.
29545893	6	2	theme	current	1290:1296	arg1	study					1298:1302	the current study	1286:1302	the current study	1286:1302	The results of the current study demonstrated that GIPS positively adjusts the immune system, which may serve as a potential immunostimulatory agent.
29545893	6	3	theme	study	1298:1302	arg1	results					1275:1281	The results	1271:1281	The results of the current study	1271:1302	The results of the current study demonstrated that GIPS positively adjusts the immune system, which may serve as a potential immunostimulatory agent.
29545893	0	4	theme	cyclophosphamide-induced	94:117	arg1	mice					137:140	cyclophosphamide-induced immunosuppression mice	94:140	cyclophosphamide-induced immunosuppression mice	94:140	The investigation of immunomodulatory activities of Gloeostereum incaratum polysaccharides in cyclophosphamide-induced immunosuppression mice.
29545893	0	5	from	investigation	4:16	arg1	mice					137:140	cyclophosphamide-induced immunosuppression mice	94:140	cyclophosphamide-induced immunosuppression mice	94:140	The investigation of immunomodulatory activities of Gloeostereum incaratum polysaccharides in cyclophosphamide-induced immunosuppression mice.
29545893	1	6	theme	anti-bacterial	205:218	arg1	activities					242:251	anti-bacterial and anti-inflammatory activities	205:251	anti-bacterial and anti-inflammatory activities	205:251	Gloeostereum incarnatum, a precious edible mushroom, displays anti-bacterial and anti-inflammatory activities; however, its immunomodulatory effect has not been studied yet.
29545893	4	7	from	-α	957:958	arg1	spleen					1013:1018	the spleen	1009:1018	the spleen	1009:1018	GIPS significantly raised the serum levels of immunoglobulin (Ig)A and IgG, promoted the production of interleukins (ILs), including IL-2, IL-3 and IL-6, interferons, including interferon (IFN)-α and IFN-γ, and monocyte chemotactic protein 1 in the spleen, which resulted in accelerating recovery of immunosuppression.
29545893	5	8	theme	dismutase	1165:1173	arg1	levels					1175:1180	the increased superoxide dismutase levels	1140:1180	the increased superoxide dismutase levels in the serum and spleen	1140:1204	Finally, GIPS showed anti-oxidative effects indicated by the increased superoxide dismutase levels in the serum and spleen, and the reduced level of reactive oxygen species in the spleen.
29545893	5	9	from	levels	1175:1180	arg1	spleen					1199:1204	spleen	1199:1204	spleen	1199:1204	Finally, GIPS showed anti-oxidative effects indicated by the increased superoxide dismutase levels in the serum and spleen, and the reduced level of reactive oxygen species in the spleen.
29545893	5	9	from	levels	1175:1180	arg1	serum					1189:1193	serum	1189:1193	serum	1189:1193	Finally, GIPS showed anti-oxidative effects indicated by the increased superoxide dismutase levels in the serum and spleen, and the reduced level of reactive oxygen species in the spleen.
29545893	3	10	from	doses	587:591	arg1	administration					569:582	The 28-day GIPS administration	553:582	The 28-day GIPS administration at doses of 0.1, 0.3 and 0.9 g/kg	553:616	The 28-day GIPS administration at doses of 0.1, 0.3 and 0.9 g/kg remarkably reversed the bodyweight loss, increased the thymic index and promoted T lymphocyte proliferation in CTX-induced immunosuppressed mice.
29545893	4	11	dep	raised	783:788	arg1	promoted					840:847	promoted	840:847	promoted the production of interleukins (ILs), including IL-2, IL-3 and IL-6, interferons, including interferon (IFN)-α and IFN-γ, and monocyte chemotactic protein 1 in the spleen, which resulted in accelerating recovery of immunosuppression	840:1080	GIPS significantly raised the serum levels of immunoglobulin (Ig)A and IgG, promoted the production of interleukins (ILs), including IL-2, IL-3 and IL-6, interferons, including interferon (IFN)-α and IFN-γ, and monocyte chemotactic protein 1 in the spleen, which resulted in accelerating recovery of immunosuppression.
29545893	4	12	theme	immunosuppression	1064:1080	arg1	recovery					1052:1059	recovery	1052:1059	recovery of immunosuppression	1052:1080	GIPS significantly raised the serum levels of immunoglobulin (Ig)A and IgG, promoted the production of interleukins (ILs), including IL-2, IL-3 and IL-6, interferons, including interferon (IFN)-α and IFN-γ, and monocyte chemotactic protein 1 in the spleen, which resulted in accelerating recovery of immunosuppression.
29545893	4	13	from	IFN-γ	964:968	arg1	spleen					1013:1018	the spleen	1009:1018	the spleen	1009:1018	GIPS significantly raised the serum levels of immunoglobulin (Ig)A and IgG, promoted the production of interleukins (ILs), including IL-2, IL-3 and IL-6, interferons, including interferon (IFN)-α and IFN-γ, and monocyte chemotactic protein 1 in the spleen, which resulted in accelerating recovery of immunosuppression.
29545893	5	14	theme	anti-oxidative	1104:1117	arg1	effects					1119:1125	anti-oxidative effects	1104:1125	anti-oxidative effects indicated by the increased superoxide dismutase levels in the serum and spleen	1104:1204	Finally, GIPS showed anti-oxidative effects indicated by the increased superoxide dismutase levels in the serum and spleen, and the reduced level of reactive oxygen species in the spleen.
29545893	4	15	theme	A	829:829	arg1	levels					800:805	the serum levels	790:805	the serum levels of immunoglobulin (Ig)A and IgG	790:837	GIPS significantly raised the serum levels of immunoglobulin (Ig)A and IgG, promoted the production of interleukins (ILs), including IL-2, IL-3 and IL-6, interferons, including interferon (IFN)-α and IFN-γ, and monocyte chemotactic protein 1 in the spleen, which resulted in accelerating recovery of immunosuppression.
29545893	3	16	theme	28-day	557:562	arg1	administration					569:582	The 28-day GIPS administration	553:582	The 28-day GIPS administration at doses of 0.1, 0.3 and 0.9 g/kg	553:616	The 28-day GIPS administration at doses of 0.1, 0.3 and 0.9 g/kg remarkably reversed the bodyweight loss, increased the thymic index and promoted T lymphocyte proliferation in CTX-induced immunosuppressed mice.
29545893	3	17	theme	GIPS	564:567	arg1	administration					569:582	The 28-day GIPS administration	553:582	The 28-day GIPS administration at doses of 0.1, 0.3 and 0.9 g/kg	553:616	The 28-day GIPS administration at doses of 0.1, 0.3 and 0.9 g/kg remarkably reversed the bodyweight loss, increased the thymic index and promoted T lymphocyte proliferation in CTX-induced immunosuppressed mice.
29545893	2	18	theme	BALB/c	534:539	arg1	model					546:550	a Cyclophosphamide monohydrate (CTX)-induced BALB/c mice model	489:550	a Cyclophosphamide monohydrate (CTX)-induced BALB/c mice model	489:550	The present study aimed to investigate whether polysaccharide compositions of G. incarnatum polysaccharides (GIPS) possess immunomodulatory and immuno-enhancing effects in a Cyclophosphamide monohydrate (CTX)-induced BALB/c mice model.
29545893	5	19	theme	oxygen	1241:1246	arg1	species					1248:1254	reactive oxygen species	1232:1254	reactive oxygen species	1232:1254	Finally, GIPS showed anti-oxidative effects indicated by the increased superoxide dismutase levels in the serum and spleen, and the reduced level of reactive oxygen species in the spleen.
29545893	2	20	theme	present	321:327	arg1	study					329:333	The present study	317:333	The present study	317:333	The present study aimed to investigate whether polysaccharide compositions of G. incarnatum polysaccharides (GIPS) possess immunomodulatory and immuno-enhancing effects in a Cyclophosphamide monohydrate (CTX)-induced BALB/c mice model.
29545893	1	21	theme	anti-inflammatory	224:240	arg1	activities					242:251	anti-bacterial and anti-inflammatory activities	205:251	anti-bacterial and anti-inflammatory activities	205:251	Gloeostereum incarnatum, a precious edible mushroom, displays anti-bacterial and anti-inflammatory activities; however, its immunomodulatory effect has not been studied yet.
29545893	2	22	theme	-induced	525:532	arg1	model					546:550	a Cyclophosphamide monohydrate (CTX)-induced BALB/c mice model	489:550	a Cyclophosphamide monohydrate (CTX)-induced BALB/c mice model	489:550	The present study aimed to investigate whether polysaccharide compositions of G. incarnatum polysaccharides (GIPS) possess immunomodulatory and immuno-enhancing effects in a Cyclophosphamide monohydrate (CTX)-induced BALB/c mice model.
29545893	2	23	contain	possess	432:438	arg2	effects					478:484	immunomodulatory and immuno-enhancing effects	440:484	immunomodulatory and immuno-enhancing effects	440:484	The present study aimed to investigate whether polysaccharide compositions of G. incarnatum polysaccharides (GIPS) possess immunomodulatory and immuno-enhancing effects in a Cyclophosphamide monohydrate (CTX)-induced BALB/c mice model.
29545893	2	23	contain	possess	432:438	arg1	compositions					379:390	polysaccharide compositions	364:390	polysaccharide compositions of G. incarnatum polysaccharides (GIPS)	364:430	The present study aimed to investigate whether polysaccharide compositions of G. incarnatum polysaccharides (GIPS) possess immunomodulatory and immuno-enhancing effects in a Cyclophosphamide monohydrate (CTX)-induced BALB/c mice model.
29545893	6	24	theme	potential	1386:1394	arg1	system					1357:1362	the immune system	1346:1362	the immune system	1346:1362	The results of the current study demonstrated that GIPS positively adjusts the immune system, which may serve as a potential immunostimulatory agent.
29545893	6	24	theme	potential	1386:1394	arg1	agent					1414:1418	a potential immunostimulatory agent	1384:1418	a potential immunostimulatory agent	1384:1418	The results of the current study demonstrated that GIPS positively adjusts the immune system, which may serve as a potential immunostimulatory agent.
29545893	5	25	dep	serum	1189:1193	arg1	the					1185:1187	the	1185:1187	the	1185:1187	Finally, GIPS showed anti-oxidative effects indicated by the increased superoxide dismutase levels in the serum and spleen, and the reduced level of reactive oxygen species in the spleen.
29545893	5	26	theme	superoxide	1154:1163	arg1	dismutase					1165:1173	superoxide dismutase	1154:1173	the increased superoxide dismutase levels in the serum and spleen	1140:1204	Finally, GIPS showed anti-oxidative effects indicated by the increased superoxide dismutase levels in the serum and spleen, and the reduced level of reactive oxygen species in the spleen.
29545893	6	27	theme	immune	1350:1355	arg1	system					1357:1362	the immune system	1346:1362	the immune system	1346:1362	The results of the current study demonstrated that GIPS positively adjusts the immune system, which may serve as a potential immunostimulatory agent.
29545893	6	27	theme	immune	1350:1355	arg1	agent					1414:1418	a potential immunostimulatory agent	1384:1418	a potential immunostimulatory agent	1384:1418	The results of the current study demonstrated that GIPS positively adjusts the immune system, which may serve as a potential immunostimulatory agent.
29545893	2	28	theme	polysaccharide	364:377	arg1	compositions					379:390	polysaccharide compositions	364:390	polysaccharide compositions of G. incarnatum polysaccharides (GIPS)	364:430	The present study aimed to investigate whether polysaccharide compositions of G. incarnatum polysaccharides (GIPS) possess immunomodulatory and immuno-enhancing effects in a Cyclophosphamide monohydrate (CTX)-induced BALB/c mice model.
29545893	2	29	theme	immunomodulatory	440:455	arg1	effects					478:484	immunomodulatory and immuno-enhancing effects	440:484	immunomodulatory and immuno-enhancing effects	440:484	The present study aimed to investigate whether polysaccharide compositions of G. incarnatum polysaccharides (GIPS) possess immunomodulatory and immuno-enhancing effects in a Cyclophosphamide monohydrate (CTX)-induced BALB/c mice model.
29545893	0	30	theme	immunomodulatory	21:36	arg1	activities					38:47	immunomodulatory activities	21:47	immunomodulatory activities of Gloeostereum incaratum polysaccharides	21:89	The investigation of immunomodulatory activities of Gloeostereum incaratum polysaccharides in cyclophosphamide-induced immunosuppression mice.
29545893	4	31	theme	IFN	953:955	arg1	-α					957:958	interferon (IFN)-α	941:958	interferon (IFN)-α	941:958	GIPS significantly raised the serum levels of immunoglobulin (Ig)A and IgG, promoted the production of interleukins (ILs), including IL-2, IL-3 and IL-6, interferons, including interferon (IFN)-α and IFN-γ, and monocyte chemotactic protein 1 in the spleen, which resulted in accelerating recovery of immunosuppression.
29545893	1	32	theme	Gloeostereum	143:154	arg1	mushroom					186:193	a precious edible mushroom	168:193	a precious edible mushroom	168:193	Gloeostereum incarnatum, a precious edible mushroom, displays anti-bacterial and anti-inflammatory activities; however, its immunomodulatory effect has not been studied yet.
29545893	1	32	theme	Gloeostereum	143:154	arg1	incarnatum					156:165	Gloeostereum incarnatum	143:165	Gloeostereum incarnatum	143:165	Gloeostereum incarnatum, a precious edible mushroom, displays anti-bacterial and anti-inflammatory activities; however, its immunomodulatory effect has not been studied yet.
29545893	4	33	theme	immunoglobulin	810:823	arg1	A					829:829	immunoglobulin (Ig)A	810:829	immunoglobulin (Ig)A	810:829	GIPS significantly raised the serum levels of immunoglobulin (Ig)A and IgG, promoted the production of interleukins (ILs), including IL-2, IL-3 and IL-6, interferons, including interferon (IFN)-α and IFN-γ, and monocyte chemotactic protein 1 in the spleen, which resulted in accelerating recovery of immunosuppression.
29545893	3	34	theme	lymphocyte	701:710	arg1	proliferation					712:724	T lymphocyte proliferation	699:724	T lymphocyte proliferation	699:724	The 28-day GIPS administration at doses of 0.1, 0.3 and 0.9 g/kg remarkably reversed the bodyweight loss, increased the thymic index and promoted T lymphocyte proliferation in CTX-induced immunosuppressed mice.
29545893	2	35	theme	Cyclophosphamide	491:506	arg1	CTX					521:523	CTX	521:523	CTX	521:523	The present study aimed to investigate whether polysaccharide compositions of G. incarnatum polysaccharides (GIPS) possess immunomodulatory and immuno-enhancing effects in a Cyclophosphamide monohydrate (CTX)-induced BALB/c mice model.
29545893	2	35	theme	Cyclophosphamide	491:506	arg1	monohydrate					508:518	Cyclophosphamide monohydrate	491:518	Cyclophosphamide monohydrate (CTX)	491:524	The present study aimed to investigate whether polysaccharide compositions of G. incarnatum polysaccharides (GIPS) possess immunomodulatory and immuno-enhancing effects in a Cyclophosphamide monohydrate (CTX)-induced BALB/c mice model.
29545893	0	36	theme	activities	38:47	arg1	investigation					4:16	The investigation	0:16	The investigation of immunomodulatory activities of Gloeostereum incaratum polysaccharides in cyclophosphamide-induced immunosuppression mice.	0:141	The investigation of immunomodulatory activities of Gloeostereum incaratum polysaccharides in cyclophosphamide-induced immunosuppression mice.
29545893	5	37	theme	reduced	1215:1221	arg1	level					1223:1227	the reduced level	1211:1227	the reduced level of reactive oxygen species in the spleen	1211:1268	Finally, GIPS showed anti-oxidative effects indicated by the increased superoxide dismutase levels in the serum and spleen, and the reduced level of reactive oxygen species in the spleen.
29545893	4	38	theme	interleukins	867:878	arg1	production					853:862	the production	849:862	the production of interleukins (ILs), including IL-2, IL-3 and IL-6, interferons, including interferon (IFN)-α and IFN-γ, and monocyte chemotactic protein 1 in the spleen, which resulted in accelerating recovery of immunosuppression	849:1080	GIPS significantly raised the serum levels of immunoglobulin (Ig)A and IgG, promoted the production of interleukins (ILs), including IL-2, IL-3 and IL-6, interferons, including interferon (IFN)-α and IFN-γ, and monocyte chemotactic protein 1 in the spleen, which resulted in accelerating recovery of immunosuppression.
29545893	5	39	from	effects	1119:1125	arg1	spleen					1263:1268	the spleen	1259:1268	the spleen	1259:1268	Finally, GIPS showed anti-oxidative effects indicated by the increased superoxide dismutase levels in the serum and spleen, and the reduced level of reactive oxygen species in the spleen.
29545893	1	40	theme	immunomodulatory	267:282	arg1	effect					284:289	its immunomodulatory effect	263:289	its immunomodulatory effect	263:289	Gloeostereum incarnatum, a precious edible mushroom, displays anti-bacterial and anti-inflammatory activities; however, its immunomodulatory effect has not been studied yet.
29545893	2	41	theme	mice	541:544	arg1	model					546:550	a Cyclophosphamide monohydrate (CTX)-induced BALB/c mice model	489:550	a Cyclophosphamide monohydrate (CTX)-induced BALB/c mice model	489:550	The present study aimed to investigate whether polysaccharide compositions of G. incarnatum polysaccharides (GIPS) possess immunomodulatory and immuno-enhancing effects in a Cyclophosphamide monohydrate (CTX)-induced BALB/c mice model.
29545893	0	42	theme	polysaccharides	75:89	arg1	activities					38:47	immunomodulatory activities	21:47	immunomodulatory activities of Gloeostereum incaratum polysaccharides	21:89	The investigation of immunomodulatory activities of Gloeostereum incaratum polysaccharides in cyclophosphamide-induced immunosuppression mice.
29545893	3	43	theme	bodyweight	642:651	arg1	loss					653:656	the bodyweight loss	638:656	the bodyweight loss	638:656	The 28-day GIPS administration at doses of 0.1, 0.3 and 0.9 g/kg remarkably reversed the bodyweight loss, increased the thymic index and promoted T lymphocyte proliferation in CTX-induced immunosuppressed mice.
29545893	4	44	theme	interferon	941:950	arg1	-α					957:958	interferon (IFN)-α	941:958	interferon (IFN)-α	941:958	GIPS significantly raised the serum levels of immunoglobulin (Ig)A and IgG, promoted the production of interleukins (ILs), including IL-2, IL-3 and IL-6, interferons, including interferon (IFN)-α and IFN-γ, and monocyte chemotactic protein 1 in the spleen, which resulted in accelerating recovery of immunosuppression.
29545893	2	45	theme	polysaccharides	409:423	arg1	compositions					379:390	polysaccharide compositions	364:390	polysaccharide compositions of G. incarnatum polysaccharides (GIPS)	364:430	The present study aimed to investigate whether polysaccharide compositions of G. incarnatum polysaccharides (GIPS) possess immunomodulatory and immuno-enhancing effects in a Cyclophosphamide monohydrate (CTX)-induced BALB/c mice model.
29545893	0	46	theme	Gloeostereum	52:63	arg1	polysaccharides					75:89	Gloeostereum incaratum polysaccharides	52:89	Gloeostereum incaratum polysaccharides	52:89	The investigation of immunomodulatory activities of Gloeostereum incaratum polysaccharides in cyclophosphamide-induced immunosuppression mice.
29545893	5	47	from	level	1223:1227	arg1	spleen					1263:1268	the spleen	1259:1268	the spleen	1259:1268	Finally, GIPS showed anti-oxidative effects indicated by the increased superoxide dismutase levels in the serum and spleen, and the reduced level of reactive oxygen species in the spleen.
29545893	3	48	theme	T	699:699	arg1	proliferation					712:724	T lymphocyte proliferation	699:724	T lymphocyte proliferation	699:724	The 28-day GIPS administration at doses of 0.1, 0.3 and 0.9 g/kg remarkably reversed the bodyweight loss, increased the thymic index and promoted T lymphocyte proliferation in CTX-induced immunosuppressed mice.
29545893	4	49	theme	monocyte	975:982	arg1	protein					996:1002	monocyte chemotactic protein 1	975:1004	monocyte chemotactic protein 1	975:1004	GIPS significantly raised the serum levels of immunoglobulin (Ig)A and IgG, promoted the production of interleukins (ILs), including IL-2, IL-3 and IL-6, interferons, including interferon (IFN)-α and IFN-γ, and monocyte chemotactic protein 1 in the spleen, which resulted in accelerating recovery of immunosuppression.
29545893	2	50	theme	incarnatum	398:407	arg1	GIPS					426:429	GIPS	426:429	GIPS	426:429	The present study aimed to investigate whether polysaccharide compositions of G. incarnatum polysaccharides (GIPS) possess immunomodulatory and immuno-enhancing effects in a Cyclophosphamide monohydrate (CTX)-induced BALB/c mice model.
29545893	2	50	theme	incarnatum	398:407	arg1	polysaccharides					409:423	G. incarnatum polysaccharides	395:423	G. incarnatum polysaccharides (GIPS)	395:430	The present study aimed to investigate whether polysaccharide compositions of G. incarnatum polysaccharides (GIPS) possess immunomodulatory and immuno-enhancing effects in a Cyclophosphamide monohydrate (CTX)-induced BALB/c mice model.
29545893	4	51	from	protein	996:1002	arg1	spleen					1013:1018	the spleen	1009:1018	the spleen	1009:1018	GIPS significantly raised the serum levels of immunoglobulin (Ig)A and IgG, promoted the production of interleukins (ILs), including IL-2, IL-3 and IL-6, interferons, including interferon (IFN)-α and IFN-γ, and monocyte chemotactic protein 1 in the spleen, which resulted in accelerating recovery of immunosuppression.
29545893	4	52	theme	serum	794:798	arg1	levels					800:805	the serum levels	790:805	the serum levels of immunoglobulin (Ig)A and IgG	790:837	GIPS significantly raised the serum levels of immunoglobulin (Ig)A and IgG, promoted the production of interleukins (ILs), including IL-2, IL-3 and IL-6, interferons, including interferon (IFN)-α and IFN-γ, and monocyte chemotactic protein 1 in the spleen, which resulted in accelerating recovery of immunosuppression.
29545893	1	53	theme	precious	170:177	arg1	mushroom					186:193	a precious edible mushroom	168:193	a precious edible mushroom	168:193	Gloeostereum incarnatum, a precious edible mushroom, displays anti-bacterial and anti-inflammatory activities; however, its immunomodulatory effect has not been studied yet.
29545893	1	53	theme	precious	170:177	arg1	incarnatum					156:165	Gloeostereum incarnatum	143:165	Gloeostereum incarnatum	143:165	Gloeostereum incarnatum, a precious edible mushroom, displays anti-bacterial and anti-inflammatory activities; however, its immunomodulatory effect has not been studied yet.
29545893	3	54	theme	g/kg	613:616	arg1	doses					587:591	doses	587:591	doses of 0.1, 0.3 and 0.9 g/kg	587:616	The 28-day GIPS administration at doses of 0.1, 0.3 and 0.9 g/kg remarkably reversed the bodyweight loss, increased the thymic index and promoted T lymphocyte proliferation in CTX-induced immunosuppressed mice.
29545893	5	55	theme	reactive	1232:1239	arg1	species					1248:1254	reactive oxygen species	1232:1254	reactive oxygen species	1232:1254	Finally, GIPS showed anti-oxidative effects indicated by the increased superoxide dismutase levels in the serum and spleen, and the reduced level of reactive oxygen species in the spleen.
29545893	4	56	theme	IgG	835:837	arg1	levels					800:805	the serum levels	790:805	the serum levels of immunoglobulin (Ig)A and IgG	790:837	GIPS significantly raised the serum levels of immunoglobulin (Ig)A and IgG, promoted the production of interleukins (ILs), including IL-2, IL-3 and IL-6, interferons, including interferon (IFN)-α and IFN-γ, and monocyte chemotactic protein 1 in the spleen, which resulted in accelerating recovery of immunosuppression.
29545893	1	57	theme	edible	179:184	arg1	mushroom					186:193	a precious edible mushroom	168:193	a precious edible mushroom	168:193	Gloeostereum incarnatum, a precious edible mushroom, displays anti-bacterial and anti-inflammatory activities; however, its immunomodulatory effect has not been studied yet.
29545893	1	57	theme	edible	179:184	arg1	incarnatum					156:165	Gloeostereum incarnatum	143:165	Gloeostereum incarnatum	143:165	Gloeostereum incarnatum, a precious edible mushroom, displays anti-bacterial and anti-inflammatory activities; however, its immunomodulatory effect has not been studied yet.
29545893	2	58	theme	G.	395:396	arg1	GIPS					426:429	GIPS	426:429	GIPS	426:429	The present study aimed to investigate whether polysaccharide compositions of G. incarnatum polysaccharides (GIPS) possess immunomodulatory and immuno-enhancing effects in a Cyclophosphamide monohydrate (CTX)-induced BALB/c mice model.
29545893	2	58	theme	G.	395:396	arg1	polysaccharides					409:423	G. incarnatum polysaccharides	395:423	G. incarnatum polysaccharides (GIPS)	395:430	The present study aimed to investigate whether polysaccharide compositions of G. incarnatum polysaccharides (GIPS) possess immunomodulatory and immuno-enhancing effects in a Cyclophosphamide monohydrate (CTX)-induced BALB/c mice model.
29545893	4	59	theme	chemotactic	984:994	arg1	protein					996:1002	monocyte chemotactic protein 1	975:1004	monocyte chemotactic protein 1	975:1004	GIPS significantly raised the serum levels of immunoglobulin (Ig)A and IgG, promoted the production of interleukins (ILs), including IL-2, IL-3 and IL-6, interferons, including interferon (IFN)-α and IFN-γ, and monocyte chemotactic protein 1 in the spleen, which resulted in accelerating recovery of immunosuppression.
29545893	3	60	theme	CTX-induced	729:739	arg1	mice					758:761	CTX-induced immunosuppressed mice	729:761	CTX-induced immunosuppressed mice	729:761	The 28-day GIPS administration at doses of 0.1, 0.3 and 0.9 g/kg remarkably reversed the bodyweight loss, increased the thymic index and promoted T lymphocyte proliferation in CTX-induced immunosuppressed mice.
29545893	6	61	theme	immunostimulatory	1396:1412	arg1	system					1357:1362	the immune system	1346:1362	the immune system	1346:1362	The results of the current study demonstrated that GIPS positively adjusts the immune system, which may serve as a potential immunostimulatory agent.
29545893	6	61	theme	immunostimulatory	1396:1412	arg1	agent					1414:1418	a potential immunostimulatory agent	1384:1418	a potential immunostimulatory agent	1384:1418	The results of the current study demonstrated that GIPS positively adjusts the immune system, which may serve as a potential immunostimulatory agent.
29545893	2	62	theme	immuno-enhancing	461:476	arg1	effects					478:484	immunomodulatory and immuno-enhancing effects	440:484	immunomodulatory and immuno-enhancing effects	440:484	The present study aimed to investigate whether polysaccharide compositions of G. incarnatum polysaccharides (GIPS) possess immunomodulatory and immuno-enhancing effects in a Cyclophosphamide monohydrate (CTX)-induced BALB/c mice model.
29545893	3	63	theme	immunosuppressed	741:756	arg1	mice					758:761	CTX-induced immunosuppressed mice	729:761	CTX-induced immunosuppressed mice	729:761	The 28-day GIPS administration at doses of 0.1, 0.3 and 0.9 g/kg remarkably reversed the bodyweight loss, increased the thymic index and promoted T lymphocyte proliferation in CTX-induced immunosuppressed mice.
29545893	0	64	theme	incaratum	65:73	arg1	polysaccharides					75:89	Gloeostereum incaratum polysaccharides	52:89	Gloeostereum incaratum polysaccharides	52:89	The investigation of immunomodulatory activities of Gloeostereum incaratum polysaccharides in cyclophosphamide-induced immunosuppression mice.
29545893	3	65	theme	thymic	673:678	arg1	index					680:684	the thymic index	669:684	the thymic index	669:684	The 28-day GIPS administration at doses of 0.1, 0.3 and 0.9 g/kg remarkably reversed the bodyweight loss, increased the thymic index and promoted T lymphocyte proliferation in CTX-induced immunosuppressed mice.
29545893	5	66	theme	species	1248:1254	arg1	level					1223:1227	the reduced level	1211:1227	the reduced level of reactive oxygen species in the spleen	1211:1268	Finally, GIPS showed anti-oxidative effects indicated by the increased superoxide dismutase levels in the serum and spleen, and the reduced level of reactive oxygen species in the spleen.
29545893	5	66	theme	species	1248:1254	arg1	effects					1119:1125	anti-oxidative effects	1104:1125	anti-oxidative effects indicated by the increased superoxide dismutase levels in the serum and spleen	1104:1204	Finally, GIPS showed anti-oxidative effects indicated by the increased superoxide dismutase levels in the serum and spleen, and the reduced level of reactive oxygen species in the spleen.
30946397	1	0	theme	μM	193:194	arg1	affinities					204:213	20-140 μM binding affinities	186:213	20-140 μM binding affinities	186:213	Nanoparticle receptors were synthesized through micellar imprinting to bind glycosphingolipids with 20-140 μM binding affinities, meanwhile distinguishing glycan composition, the number of acyl chains, and hydroxylation of acyl chains in the lipids.
30946397	1	1	theme	binding	196:202	arg1	affinities					204:213	20-140 μM binding affinities	186:213	20-140 μM binding affinities	186:213	Nanoparticle receptors were synthesized through micellar imprinting to bind glycosphingolipids with 20-140 μM binding affinities, meanwhile distinguishing glycan composition, the number of acyl chains, and hydroxylation of acyl chains in the lipids.
30946397	1	2	from	lipids	328:333	arg1	composition					248:258	meanwhile distinguishing glycan composition	216:258	meanwhile distinguishing glycan composition	216:258	Nanoparticle receptors were synthesized through micellar imprinting to bind glycosphingolipids with 20-140 μM binding affinities, meanwhile distinguishing glycan composition, the number of acyl chains, and hydroxylation of acyl chains in the lipids.
30946397	1	2	from	lipids	328:333	arg1	affinities					204:213	20-140 μM binding affinities	186:213	20-140 μM binding affinities	186:213	Nanoparticle receptors were synthesized through micellar imprinting to bind glycosphingolipids with 20-140 μM binding affinities, meanwhile distinguishing glycan composition, the number of acyl chains, and hydroxylation of acyl chains in the lipids.
30946397	1	2	from	lipids	328:333	arg1	hydroxylation					292:304	hydroxylation	292:304	hydroxylation of acyl chains in the lipids	292:333	Nanoparticle receptors were synthesized through micellar imprinting to bind glycosphingolipids with 20-140 μM binding affinities, meanwhile distinguishing glycan composition, the number of acyl chains, and hydroxylation of acyl chains in the lipids.
30946397	1	2	from	lipids	328:333	arg1	number					265:270	the number	261:270	the number of acyl chains	261:285	Nanoparticle receptors were synthesized through micellar imprinting to bind glycosphingolipids with 20-140 μM binding affinities, meanwhile distinguishing glycan composition, the number of acyl chains, and hydroxylation of acyl chains in the lipids.
30946397	1	3	theme	Nanoparticle	86:97	arg1	receptors					99:107	Nanoparticle receptors	86:107	Nanoparticle receptors	86:107	Nanoparticle receptors were synthesized through micellar imprinting to bind glycosphingolipids with 20-140 μM binding affinities, meanwhile distinguishing glycan composition, the number of acyl chains, and hydroxylation of acyl chains in the lipids.
30946397	2	4	theme	lipid	426:430	arg1	membranes					432:440	lipid membranes	426:440	lipid membranes from enzymatic degradation	426:467	The strong binding enabled the receptors to protect their target glycolipids dispersed in lipid membranes from enzymatic degradation.
30946397	1	5	with	glycosphingolipids	162:179	arg1	composition					248:258	meanwhile distinguishing glycan composition	216:258	meanwhile distinguishing glycan composition	216:258	Nanoparticle receptors were synthesized through micellar imprinting to bind glycosphingolipids with 20-140 μM binding affinities, meanwhile distinguishing glycan composition, the number of acyl chains, and hydroxylation of acyl chains in the lipids.
30946397	1	5	with	glycosphingolipids	162:179	arg1	number					265:270	the number	261:270	the number of acyl chains	261:285	Nanoparticle receptors were synthesized through micellar imprinting to bind glycosphingolipids with 20-140 μM binding affinities, meanwhile distinguishing glycan composition, the number of acyl chains, and hydroxylation of acyl chains in the lipids.
30946397	1	5	with	glycosphingolipids	162:179	arg1	hydroxylation					292:304	hydroxylation	292:304	hydroxylation of acyl chains in the lipids	292:333	Nanoparticle receptors were synthesized through micellar imprinting to bind glycosphingolipids with 20-140 μM binding affinities, meanwhile distinguishing glycan composition, the number of acyl chains, and hydroxylation of acyl chains in the lipids.
30946397	1	5	with	glycosphingolipids	162:179	arg1	affinities					204:213	20-140 μM binding affinities	186:213	20-140 μM binding affinities	186:213	Nanoparticle receptors were synthesized through micellar imprinting to bind glycosphingolipids with 20-140 μM binding affinities, meanwhile distinguishing glycan composition, the number of acyl chains, and hydroxylation of acyl chains in the lipids.
30946397	2	6	theme	strong	340:345	arg1	binding					347:353	The strong binding	336:353	The strong binding	336:353	The strong binding enabled the receptors to protect their target glycolipids dispersed in lipid membranes from enzymatic degradation.
30946397	1	7	theme	meanwhile	216:224	arg1	composition					248:258	meanwhile distinguishing glycan composition	216:258	meanwhile distinguishing glycan composition	216:258	Nanoparticle receptors were synthesized through micellar imprinting to bind glycosphingolipids with 20-140 μM binding affinities, meanwhile distinguishing glycan composition, the number of acyl chains, and hydroxylation of acyl chains in the lipids.
30946397	1	8	theme	acyl	309:312	arg1	chains					314:319	acyl chains	309:319	acyl chains in the lipids	309:333	Nanoparticle receptors were synthesized through micellar imprinting to bind glycosphingolipids with 20-140 μM binding affinities, meanwhile distinguishing glycan composition, the number of acyl chains, and hydroxylation of acyl chains in the lipids.
30946397	1	9	from	chains	314:319	arg1	lipids					328:333	the lipids	324:333	the lipids	324:333	Nanoparticle receptors were synthesized through micellar imprinting to bind glycosphingolipids with 20-140 μM binding affinities, meanwhile distinguishing glycan composition, the number of acyl chains, and hydroxylation of acyl chains in the lipids.
30946397	1	10	theme	distinguishing	226:239	arg1	composition					248:258	meanwhile distinguishing glycan composition	216:258	meanwhile distinguishing glycan composition	216:258	Nanoparticle receptors were synthesized through micellar imprinting to bind glycosphingolipids with 20-140 μM binding affinities, meanwhile distinguishing glycan composition, the number of acyl chains, and hydroxylation of acyl chains in the lipids.
30946397	1	11	theme	chains	314:319	arg1	composition					248:258	meanwhile distinguishing glycan composition	216:258	meanwhile distinguishing glycan composition	216:258	Nanoparticle receptors were synthesized through micellar imprinting to bind glycosphingolipids with 20-140 μM binding affinities, meanwhile distinguishing glycan composition, the number of acyl chains, and hydroxylation of acyl chains in the lipids.
30946397	1	11	theme	chains	314:319	arg1	affinities					204:213	20-140 μM binding affinities	186:213	20-140 μM binding affinities	186:213	Nanoparticle receptors were synthesized through micellar imprinting to bind glycosphingolipids with 20-140 μM binding affinities, meanwhile distinguishing glycan composition, the number of acyl chains, and hydroxylation of acyl chains in the lipids.
30946397	1	11	theme	chains	314:319	arg1	hydroxylation					292:304	hydroxylation	292:304	hydroxylation of acyl chains in the lipids	292:333	Nanoparticle receptors were synthesized through micellar imprinting to bind glycosphingolipids with 20-140 μM binding affinities, meanwhile distinguishing glycan composition, the number of acyl chains, and hydroxylation of acyl chains in the lipids.
30946397	1	11	theme	chains	314:319	arg1	number					265:270	the number	261:270	the number of acyl chains	261:285	Nanoparticle receptors were synthesized through micellar imprinting to bind glycosphingolipids with 20-140 μM binding affinities, meanwhile distinguishing glycan composition, the number of acyl chains, and hydroxylation of acyl chains in the lipids.
30946397	1	12	from	hydroxylation	292:304	arg1	lipids					328:333	the lipids	324:333	the lipids	324:333	Nanoparticle receptors were synthesized through micellar imprinting to bind glycosphingolipids with 20-140 μM binding affinities, meanwhile distinguishing glycan composition, the number of acyl chains, and hydroxylation of acyl chains in the lipids.
30946397	1	13	theme	glycan	241:246	arg1	composition					248:258	meanwhile distinguishing glycan composition	216:258	meanwhile distinguishing glycan composition	216:258	Nanoparticle receptors were synthesized through micellar imprinting to bind glycosphingolipids with 20-140 μM binding affinities, meanwhile distinguishing glycan composition, the number of acyl chains, and hydroxylation of acyl chains in the lipids.
30946397	1	14	theme	micellar	134:141	arg1	imprinting					143:152	micellar imprinting	134:152	micellar imprinting	134:152	Nanoparticle receptors were synthesized through micellar imprinting to bind glycosphingolipids with 20-140 μM binding affinities, meanwhile distinguishing glycan composition, the number of acyl chains, and hydroxylation of acyl chains in the lipids.
30946397	2	15	theme	enzymatic	447:455	arg1	degradation					457:467	enzymatic degradation	447:467	enzymatic degradation	447:467	The strong binding enabled the receptors to protect their target glycolipids dispersed in lipid membranes from enzymatic degradation.
30946397	0	16	theme	glycosphingolipids	30:47	arg1	protection					16:25	protection	16:25	protection	16:25	Recognition and protection of glycosphingolipids by synthetic nanoparticle receptors.
30946397	0	16	theme	glycosphingolipids	30:47	arg1	Recognition					0:10	Recognition	0:10	Recognition	0:10	Recognition and protection of glycosphingolipids by synthetic nanoparticle receptors.
30946397	0	17	theme	nanoparticle	62:73	arg1	receptors					75:83	synthetic nanoparticle receptors	52:83	synthetic nanoparticle receptors	52:83	Recognition and protection of glycosphingolipids by synthetic nanoparticle receptors.
30946397	0	18	theme	synthetic	52:60	arg1	receptors					75:83	synthetic nanoparticle receptors	52:83	synthetic nanoparticle receptors	52:83	Recognition and protection of glycosphingolipids by synthetic nanoparticle receptors.
30946397	1	19	from	number	265:270	arg1	lipids					328:333	the lipids	324:333	the lipids	324:333	Nanoparticle receptors were synthesized through micellar imprinting to bind glycosphingolipids with 20-140 μM binding affinities, meanwhile distinguishing glycan composition, the number of acyl chains, and hydroxylation of acyl chains in the lipids.
30946397	1	20	from	composition	248:258	arg1	lipids					328:333	the lipids	324:333	the lipids	324:333	Nanoparticle receptors were synthesized through micellar imprinting to bind glycosphingolipids with 20-140 μM binding affinities, meanwhile distinguishing glycan composition, the number of acyl chains, and hydroxylation of acyl chains in the lipids.
30946397	2	21	from	degradation	457:467	arg1	membranes					432:440	lipid membranes	426:440	lipid membranes from enzymatic degradation	426:467	The strong binding enabled the receptors to protect their target glycolipids dispersed in lipid membranes from enzymatic degradation.
30946397	2	22	theme	target	394:399	arg1	glycolipids					401:411	their target glycolipids	388:411	their target glycolipids dispersed in lipid membranes from enzymatic degradation	388:467	The strong binding enabled the receptors to protect their target glycolipids dispersed in lipid membranes from enzymatic degradation.
30946397	1	23	theme	acyl	275:278	arg1	chains					280:285	acyl chains	275:285	acyl chains	275:285	Nanoparticle receptors were synthesized through micellar imprinting to bind glycosphingolipids with 20-140 μM binding affinities, meanwhile distinguishing glycan composition, the number of acyl chains, and hydroxylation of acyl chains in the lipids.
30946397	1	24	from	affinities	204:213	arg1	lipids					328:333	the lipids	324:333	the lipids	324:333	Nanoparticle receptors were synthesized through micellar imprinting to bind glycosphingolipids with 20-140 μM binding affinities, meanwhile distinguishing glycan composition, the number of acyl chains, and hydroxylation of acyl chains in the lipids.
30946397	1	25	theme	chains	280:285	arg1	composition					248:258	meanwhile distinguishing glycan composition	216:258	meanwhile distinguishing glycan composition	216:258	Nanoparticle receptors were synthesized through micellar imprinting to bind glycosphingolipids with 20-140 μM binding affinities, meanwhile distinguishing glycan composition, the number of acyl chains, and hydroxylation of acyl chains in the lipids.
30946397	1	25	theme	chains	280:285	arg1	affinities					204:213	20-140 μM binding affinities	186:213	20-140 μM binding affinities	186:213	Nanoparticle receptors were synthesized through micellar imprinting to bind glycosphingolipids with 20-140 μM binding affinities, meanwhile distinguishing glycan composition, the number of acyl chains, and hydroxylation of acyl chains in the lipids.
30946397	1	25	theme	chains	280:285	arg1	hydroxylation					292:304	hydroxylation	292:304	hydroxylation of acyl chains in the lipids	292:333	Nanoparticle receptors were synthesized through micellar imprinting to bind glycosphingolipids with 20-140 μM binding affinities, meanwhile distinguishing glycan composition, the number of acyl chains, and hydroxylation of acyl chains in the lipids.
30946397	1	25	theme	chains	280:285	arg1	number					265:270	the number	261:270	the number of acyl chains	261:285	Nanoparticle receptors were synthesized through micellar imprinting to bind glycosphingolipids with 20-140 μM binding affinities, meanwhile distinguishing glycan composition, the number of acyl chains, and hydroxylation of acyl chains in the lipids.
30183131	10	0	theme	containing	1651:1660	arg1	glucoside					1632:1640	glucoside	1632:1640	glucoside	1632:1640	A partial least square (PLS) biplot suggested that sulphur containing glucoside, sulphur containing compounds, phytosterols, triterpenoids, flavones and some organic and amino acids were among the potential NO inhibitors.
30183131	10	0	theme	containing	1651:1660	arg1	compounds					1662:1670	sulphur containing compounds	1643:1670	sulphur containing compounds	1643:1670	A partial least square (PLS) biplot suggested that sulphur containing glucoside, sulphur containing compounds, phytosterols, triterpenoids, flavones and some organic and amino acids were among the potential NO inhibitors.
30183131	11	1	theme	targeted	1793:1800	arg1	quantification					1802:1815	LC-MS/MS targeted quantification	1784:1815	LC-MS/MS targeted quantification	1784:1815	LC-MS/MS targeted quantification further supported sonicated water extract was among the extract that possessed the most abundant C-glycosyl flavones.
30183131	3	2	theme	post-harvesting	531:545	arg1	stage					547:551	plant post-harvesting stage	525:551	plant post-harvesting stage	525:551	Its anti-inflammation properties is influenced by the metabolites composition, which can be determined by different binary extraction solvent ratio and extraction methods used during plant post-harvesting stage.
30183131	3	3	theme	metabolites	396:406	arg1	composition					408:418	the metabolites composition	392:418	the metabolites composition	392:418	Its anti-inflammation properties is influenced by the metabolites composition, which can be determined by different binary extraction solvent ratio and extraction methods used during plant post-harvesting stage.
30183131	10	4	dep	square	1578:1583	arg1	PLS					1586:1588	PLS	1586:1588	PLS	1586:1588	A partial least square (PLS) biplot suggested that sulphur containing glucoside, sulphur containing compounds, phytosterols, triterpenoids, flavones and some organic and amino acids were among the potential NO inhibitors.
30183131	1	5	theme	activated	157:165	arg1	macrophages					177:187	LPS-IFN-γ activated RAW 264.7 macrophages	147:187	LPS-IFN-γ activated RAW 264.7 macrophages	147:187	f) Lindau water extract against nitric oxide production in LPS-IFN-γ activated RAW 264.7 macrophages.
30183131	7	6	theme	biosynthesis	1266:1277	arg1	pathway					1279:1285	The possible metabolite biosynthesis pathway	1242:1285	The possible metabolite biosynthesis pathway	1242:1285	The possible metabolite biosynthesis pathway was constructed to further strengthen the findings.
30183131	4	7	theme	magnetic	687:694	arg1	NMR					707:709	NMR	707:709	NMR	707:709	OBJECTIVE Evaluate the relationship between the chemical composition of C. nutans and its anti-inflammatory properties using nuclear magnetic resonance (NMR) metabolomics approach.
30183131	4	7	theme	magnetic	687:694	arg1	resonance					696:704	nuclear magnetic resonance	679:704	nuclear magnetic resonance (NMR) metabolomics approach	679:732	OBJECTIVE Evaluate the relationship between the chemical composition of C. nutans and its anti-inflammatory properties using nuclear magnetic resonance (NMR) metabolomics approach.
30183131	10	8	theme	organic	1720:1726	arg1	acids					1738:1742	some organic and amino acids	1715:1742	acids	1738:1742	A partial least square (PLS) biplot suggested that sulphur containing glucoside, sulphur containing compounds, phytosterols, triterpenoids, flavones and some organic and amino acids were among the potential NO inhibitors.
30183131	10	8	theme	organic	1720:1726	arg1	glucoside					1632:1640	glucoside	1632:1640	glucoside	1632:1640	A partial least square (PLS) biplot suggested that sulphur containing glucoside, sulphur containing compounds, phytosterols, triterpenoids, flavones and some organic and amino acids were among the potential NO inhibitors.
30183131	11	9	contain	possessed	1886:1894	arg2	flavones					1925:1932	the most abundant C-glycosyl flavones	1896:1932	the most abundant C-glycosyl flavones	1896:1932	LC-MS/MS targeted quantification further supported sonicated water extract was among the extract that possessed the most abundant C-glycosyl flavones.
30183131	11	9	contain	possessed	1886:1894	arg1	extract					1873:1879	the extract	1869:1879	the extract that possessed the most abundant C-glycosyl flavones	1869:1932	LC-MS/MS targeted quantification further supported sonicated water extract was among the extract that possessed the most abundant C-glycosyl flavones.
30183131	5	10	theme	lipopolysaccharide-interferon-gamma	965:999	arg1	macrophages					1033:1043	lipopolysaccharide-interferon-gamma (LPS-IFN-γ) activated RAW 264.7 macrophages	965:1043	lipopolysaccharide-interferon-gamma (LPS-IFN-γ) activated RAW 264.7 macrophages	965:1043	METHODOLOGY The anti-inflammatory effect of C. nutans air-dried leaves extracted using five different binary extraction solvent ratio and two extraction methods was determined based on their nitric oxide (NO) inhibition effect in lipopolysaccharide-interferon-gamma (LPS-IFN-γ) activated RAW 264.7 macrophages.
30183131	4	11	theme	anti-inflammatory	644:660	arg1	properties					662:671	its anti-inflammatory properties	640:671	its anti-inflammatory properties using nuclear magnetic resonance (NMR) metabolomics approach	640:732	OBJECTIVE Evaluate the relationship between the chemical composition of C. nutans and its anti-inflammatory properties using nuclear magnetic resonance (NMR) metabolomics approach.
30183131	7	12	theme	possible	1246:1253	arg1	pathway					1279:1285	The possible metabolite biosynthesis pathway	1242:1285	The possible metabolite biosynthesis pathway	1242:1285	The possible metabolite biosynthesis pathway was constructed to further strengthen the findings.
30183131	8	13	theme	highest	1408:1414	arg1	activity					1430:1437	NO inhibition activity	1416:1437	the highest NO inhibition activity (IC50 = 190.43 ± 12.26 μg/mL, P < 0.05)	1404:1477	RESULTS Water and sonication prepared air-dried leaves possessed the highest NO inhibition activity (IC50 = 190.43 ± 12.26 μg/mL, P < 0.05).
30183131	10	14	theme	amino	1732:1736	arg1	acids					1738:1742	some organic and amino acids	1715:1742	acids	1738:1742	A partial least square (PLS) biplot suggested that sulphur containing glucoside, sulphur containing compounds, phytosterols, triterpenoids, flavones and some organic and amino acids were among the potential NO inhibitors.
30183131	10	14	theme	amino	1732:1736	arg1	glucoside					1632:1640	glucoside	1632:1640	glucoside	1632:1640	A partial least square (PLS) biplot suggested that sulphur containing glucoside, sulphur containing compounds, phytosterols, triterpenoids, flavones and some organic and amino acids were among the potential NO inhibitors.
30183131	1	15	theme	water	98:102	arg1	extract					104:110	f) Lindau water extract	88:110	f) Lindau water extract against nitric oxide production in LPS-IFN-γ activated RAW 264.7 macrophages.	88:188	f) Lindau water extract against nitric oxide production in LPS-IFN-γ activated RAW 264.7 macrophages.
30183131	8	16	theme	inhibition	1419:1428	arg1	activity					1430:1437	NO inhibition activity	1416:1437	the highest NO inhibition activity (IC50 = 190.43 ± 12.26 μg/mL, P < 0.05)	1404:1477	RESULTS Water and sonication prepared air-dried leaves possessed the highest NO inhibition activity (IC50 = 190.43 ± 12.26 μg/mL, P < 0.05).
30183131	5	17	theme	nutans	782:787	arg1	effect					769:774	The anti-inflammatory effect	747:774	The anti-inflammatory effect of C. nutans air-dried	747:797	METHODOLOGY The anti-inflammatory effect of C. nutans air-dried leaves extracted using five different binary extraction solvent ratio and two extraction methods was determined based on their nitric oxide (NO) inhibition effect in lipopolysaccharide-interferon-gamma (LPS-IFN-γ) activated RAW 264.7 macrophages.
30183131	1	18	theme	oxide	127:131	arg1	production					133:142	nitric oxide production	120:142	nitric oxide production in LPS-IFN-γ activated RAW 264.7 macrophages	120:187	f) Lindau water extract against nitric oxide production in LPS-IFN-γ activated RAW 264.7 macrophages.
30183131	11	19	theme	C-glycosyl	1914:1923	arg1	flavones					1925:1932	the most abundant C-glycosyl flavones	1896:1932	the most abundant C-glycosyl flavones	1896:1932	LC-MS/MS targeted quantification further supported sonicated water extract was among the extract that possessed the most abundant C-glycosyl flavones.
30183131	0	20	theme	nutans	74:79	arg1	effect					51:56	the protective effect	36:56	the protective effect of Clinacanthus nutans (Burm	36:85	1 H-NMR metabolomics for evaluating the protective effect of Clinacanthus nutans (Burm.
30183131	1	21	from	production	133:142	arg1	macrophages					177:187	LPS-IFN-γ activated RAW 264.7 macrophages	147:187	LPS-IFN-γ activated RAW 264.7 macrophages	147:187	f) Lindau water extract against nitric oxide production in LPS-IFN-γ activated RAW 264.7 macrophages.
30183131	5	22	theme	anti-inflammatory	751:767	arg1	effect					769:774	The anti-inflammatory effect	747:774	The anti-inflammatory effect of C. nutans air-dried	747:797	METHODOLOGY The anti-inflammatory effect of C. nutans air-dried leaves extracted using five different binary extraction solvent ratio and two extraction methods was determined based on their nitric oxide (NO) inhibition effect in lipopolysaccharide-interferon-gamma (LPS-IFN-γ) activated RAW 264.7 macrophages.
30183131	5	23	theme	activated	1013:1021	arg1	macrophages					1033:1043	lipopolysaccharide-interferon-gamma (LPS-IFN-γ) activated RAW 264.7 macrophages	965:1043	lipopolysaccharide-interferon-gamma (LPS-IFN-γ) activated RAW 264.7 macrophages	965:1043	METHODOLOGY The anti-inflammatory effect of C. nutans air-dried leaves extracted using five different binary extraction solvent ratio and two extraction methods was determined based on their nitric oxide (NO) inhibition effect in lipopolysaccharide-interferon-gamma (LPS-IFN-γ) activated RAW 264.7 macrophages.
30183131	12	24	theme	extract	2030:2036	arg1	selection					2009:2017	the selection	2005:2017	the selection of optimum extract in further C. nutans in vivo anti-inflammatory study	2005:2089	CONCLUSION The present study may serve as a preliminary reference for the selection of optimum extract in further C. nutans in vivo anti-inflammatory study.
30183131	3	25	theme	anti-inflammation	346:362	arg1	properties					364:373	Its anti-inflammation properties	342:373	Its anti-inflammation properties	342:373	Its anti-inflammation properties is influenced by the metabolites composition, which can be determined by different binary extraction solvent ratio and extraction methods used during plant post-harvesting stage.
30183131	10	26	theme	potential	1759:1767	arg1	inhibitors					1772:1781	the potential NO inhibitors	1755:1781	the potential NO inhibitors	1755:1781	A partial least square (PLS) biplot suggested that sulphur containing glucoside, sulphur containing compounds, phytosterols, triterpenoids, flavones and some organic and amino acids were among the potential NO inhibitors.
30183131	5	27	theme	extraction	844:853	arg1	ratio					863:867	five different binary extraction solvent ratio and two extraction methods	822:894	ratio	863:867	METHODOLOGY The anti-inflammatory effect of C. nutans air-dried leaves extracted using five different binary extraction solvent ratio and two extraction methods was determined based on their nitric oxide (NO) inhibition effect in lipopolysaccharide-interferon-gamma (LPS-IFN-γ) activated RAW 264.7 macrophages.
30183131	12	28	theme	further	2041:2047	arg1	study					2085:2089	further C. nutans in vivo anti-inflammatory study	2041:2089	further C. nutans in vivo anti-inflammatory study	2041:2089	CONCLUSION The present study may serve as a preliminary reference for the selection of optimum extract in further C. nutans in vivo anti-inflammatory study.
30183131	0	29	dep	nutans	74:79	arg1	Burm					82:85	Burm	82:85	Burm	82:85	1 H-NMR metabolomics for evaluating the protective effect of Clinacanthus nutans (Burm.
30183131	7	30	theme	metabolite	1255:1264	arg1	pathway					1279:1285	The possible metabolite biosynthesis pathway	1242:1285	The possible metabolite biosynthesis pathway	1242:1285	The possible metabolite biosynthesis pathway was constructed to further strengthen the findings.
30183131	8	31	dep	RESULTS	1339:1345	arg1	RESULTS					1339:1345	RESULTS Water and sonication	1339:1366	RESULTS Water and sonication prepared air-dried leaves	1339:1392	RESULTS Water and sonication prepared air-dried leaves possessed the highest NO inhibition activity (IC50 = 190.43 ± 12.26 μg/mL, P < 0.05).
30183131	8	31	dep	RESULTS	1339:1345	arg1	sonication					1357:1366	sonication	1357:1366	sonication	1357:1366	RESULTS Water and sonication prepared air-dried leaves possessed the highest NO inhibition activity (IC50 = 190.43 ± 12.26 μg/mL, P < 0.05).
30183131	8	31	dep	RESULTS	1339:1345	arg1	Water					1347:1351	Water	1347:1351	Water	1347:1351	RESULTS Water and sonication prepared air-dried leaves possessed the highest NO inhibition activity (IC50 = 190.43 ± 12.26 μg/mL, P < 0.05).
30183131	1	32	theme	nitric	120:125	arg1	production					133:142	nitric oxide production	120:142	nitric oxide production in LPS-IFN-γ activated RAW 264.7 macrophages	120:187	f) Lindau water extract against nitric oxide production in LPS-IFN-γ activated RAW 264.7 macrophages.
30183131	11	33	theme	water	1845:1849	arg1	extract					1851:1857	sonicated water extract	1835:1857	sonicated water extract was among the extract that possessed the most abundant C-glycosyl flavones	1835:1932	LC-MS/MS targeted quantification further supported sonicated water extract was among the extract that possessed the most abundant C-glycosyl flavones.
30183131	6	34	theme	metabolite	1095:1104	arg1	profiles					1106:1113	extract bioactivity and metabolite profiles	1071:1113	profiles	1106:1113	The relationship between extract bioactivity and metabolite profiles and quantifications were established using 1 H-NMR metabolomics and liquid chromatography-tandem mass spectrometry (LC-MS/MS).
30183131	10	35	theme	sulphur	1643:1649	arg1	glucoside					1632:1640	glucoside	1632:1640	glucoside	1632:1640	A partial least square (PLS) biplot suggested that sulphur containing glucoside, sulphur containing compounds, phytosterols, triterpenoids, flavones and some organic and amino acids were among the potential NO inhibitors.
30183131	10	35	theme	sulphur	1643:1649	arg1	compounds					1662:1670	sulphur containing compounds	1643:1670	sulphur containing compounds	1643:1670	A partial least square (PLS) biplot suggested that sulphur containing glucoside, sulphur containing compounds, phytosterols, triterpenoids, flavones and some organic and amino acids were among the potential NO inhibitors.
30183131	3	36	theme	extraction	465:474	arg1	ratio					484:488	different binary extraction solvent ratio and extraction methods	448:511	ratio	484:488	Its anti-inflammation properties is influenced by the metabolites composition, which can be determined by different binary extraction solvent ratio and extraction methods used during plant post-harvesting stage.
30183131	5	37	theme	different	827:835	arg1	ratio					863:867	five different binary extraction solvent ratio and two extraction methods	822:894	ratio	863:867	METHODOLOGY The anti-inflammatory effect of C. nutans air-dried leaves extracted using five different binary extraction solvent ratio and two extraction methods was determined based on their nitric oxide (NO) inhibition effect in lipopolysaccharide-interferon-gamma (LPS-IFN-γ) activated RAW 264.7 macrophages.
30183131	8	38	dep	activity	1430:1437	arg1	IC50					1440:1443	IC50 = 190.43 ± 12.26 μg/mL	1440:1466	IC50 = 190.43 ± 12.26 μg/mL	1440:1466	RESULTS Water and sonication prepared air-dried leaves possessed the highest NO inhibition activity (IC50 = 190.43 ± 12.26 μg/mL, P < 0.05).
30183131	8	38	dep	activity	1430:1437	arg1	P < 0.05					1469:1476	P < 0.05	1469:1476	P < 0.05	1469:1476	RESULTS Water and sonication prepared air-dried leaves possessed the highest NO inhibition activity (IC50 = 190.43 ± 12.26 μg/mL, P < 0.05).
30183131	2	39	theme	herbal	304:309	arg1	medicine					311:318	traditional herbal medicine	292:318	traditional herbal medicine	292:318	INTRODUCTION Clinacanthus nutans, a small shrub that is native to Southeast Asia, is commonly used in traditional herbal medicine and as a food source.
30183131	9	40	theme	metabolites	1494:1504	arg1	total					1482:1486	A total	1480:1486	A total of 56 metabolites	1480:1504	A total of 56 metabolites were tentatively identified using 1 H-NMR metabolomics.
30183131	6	41	theme	chromatography-tandem	1190:1210	arg1	LC-MS/MS					1231:1238	LC-MS/MS	1231:1238	LC-MS/MS	1231:1238	The relationship between extract bioactivity and metabolite profiles and quantifications were established using 1 H-NMR metabolomics and liquid chromatography-tandem mass spectrometry (LC-MS/MS).
30183131	6	41	theme	chromatography-tandem	1190:1210	arg1	spectrometry					1217:1228	liquid chromatography-tandem mass spectrometry	1183:1228	liquid chromatography-tandem mass spectrometry (LC-MS/MS)	1183:1239	The relationship between extract bioactivity and metabolite profiles and quantifications were established using 1 H-NMR metabolomics and liquid chromatography-tandem mass spectrometry (LC-MS/MS).
30183131	2	42	used	used	284:287	arg2	source					334:339	a food source	327:339	a food source	327:339	INTRODUCTION Clinacanthus nutans, a small shrub that is native to Southeast Asia, is commonly used in traditional herbal medicine and as a food source.
30183131	2	42	used	used	284:287	arg2	native					246:251	native	246:251	native	246:251	INTRODUCTION Clinacanthus nutans, a small shrub that is native to Southeast Asia, is commonly used in traditional herbal medicine and as a food source.
30183131	2	42	used	used	284:287	arg2	INTRODUCTION					190:201	INTRODUCTION	190:201	INTRODUCTION Clinacanthus nutans	190:221	INTRODUCTION Clinacanthus nutans, a small shrub that is native to Southeast Asia, is commonly used in traditional herbal medicine and as a food source.
30183131	2	42	used	used	284:287	arg2	shrub					232:236	a small shrub	224:236	a small shrub that is native to Southeast Asia	224:269	INTRODUCTION Clinacanthus nutans, a small shrub that is native to Southeast Asia, is commonly used in traditional herbal medicine and as a food source.
30183131	4	43	theme	chemical	602:609	arg1	composition					611:621	the chemical composition	598:621	the chemical composition of C. nutans	598:634	OBJECTIVE Evaluate the relationship between the chemical composition of C. nutans and its anti-inflammatory properties using nuclear magnetic resonance (NMR) metabolomics approach.
30183131	3	44	theme	extraction	494:503	arg1	methods					505:511	different binary extraction solvent ratio and extraction methods	448:511	methods	505:511	Its anti-inflammation properties is influenced by the metabolites composition, which can be determined by different binary extraction solvent ratio and extraction methods used during plant post-harvesting stage.
30183131	12	45	theme	present	1950:1956	arg1	reference					1991:1999	a preliminary reference	1977:1999	a preliminary reference for the selection of optimum extract in further C. nutans in vivo anti-inflammatory study	1977:2089	CONCLUSION The present study may serve as a preliminary reference for the selection of optimum extract in further C. nutans in vivo anti-inflammatory study.
30183131	12	45	theme	present	1950:1956	arg1	study					1958:1962	The present study	1946:1962	The present study	1946:1962	CONCLUSION The present study may serve as a preliminary reference for the selection of optimum extract in further C. nutans in vivo anti-inflammatory study.
30183131	8	46	contain	possessed	1394:1402	arg1	RESULTS					1339:1345	RESULTS Water and sonication	1339:1366	RESULTS Water and sonication prepared air-dried leaves	1339:1392	RESULTS Water and sonication prepared air-dried leaves possessed the highest NO inhibition activity (IC50 = 190.43 ± 12.26 μg/mL, P < 0.05).
30183131	8	46	contain	possessed	1394:1402	arg1	sonication					1357:1366	sonication	1357:1366	sonication	1357:1366	RESULTS Water and sonication prepared air-dried leaves possessed the highest NO inhibition activity (IC50 = 190.43 ± 12.26 μg/mL, P < 0.05).
30183131	8	46	contain	possessed	1394:1402	arg2	activity					1430:1437	NO inhibition activity	1416:1437	the highest NO inhibition activity (IC50 = 190.43 ± 12.26 μg/mL, P < 0.05)	1404:1477	RESULTS Water and sonication prepared air-dried leaves possessed the highest NO inhibition activity (IC50 = 190.43 ± 12.26 μg/mL, P < 0.05).
30183131	8	46	contain	possessed	1394:1402	arg1	Water					1347:1351	Water	1347:1351	Water	1347:1351	RESULTS Water and sonication prepared air-dried leaves possessed the highest NO inhibition activity (IC50 = 190.43 ± 12.26 μg/mL, P < 0.05).
30183131	6	47	theme	H-NMR	1160:1164	arg1	metabolomics					1166:1177	1 H-NMR metabolomics	1158:1177	1 H-NMR metabolomics	1158:1177	The relationship between extract bioactivity and metabolite profiles and quantifications were established using 1 H-NMR metabolomics and liquid chromatography-tandem mass spectrometry (LC-MS/MS).
30183131	3	48	theme	plant	525:529	arg1	stage					547:551	plant post-harvesting stage	525:551	plant post-harvesting stage	525:551	Its anti-inflammation properties is influenced by the metabolites composition, which can be determined by different binary extraction solvent ratio and extraction methods used during plant post-harvesting stage.
30183131	9	49	theme	H-NMR	1542:1546	arg1	metabolomics					1548:1559	1 H-NMR metabolomics	1540:1559	1 H-NMR metabolomics	1540:1559	A total of 56 metabolites were tentatively identified using 1 H-NMR metabolomics.
30183131	1	50	theme	LPS-IFN-γ	147:155	arg1	macrophages					177:187	LPS-IFN-γ activated RAW 264.7 macrophages	147:187	LPS-IFN-γ activated RAW 264.7 macrophages	147:187	f) Lindau water extract against nitric oxide production in LPS-IFN-γ activated RAW 264.7 macrophages.
30183131	0	51	theme	protective	40:49	arg1	effect					51:56	the protective effect	36:56	the protective effect of Clinacanthus nutans (Burm	36:85	1 H-NMR metabolomics for evaluating the protective effect of Clinacanthus nutans (Burm.
30183131	12	52	dep	C.	2049:2050	arg1	in					2059:2060	in	2059:2060	in	2059:2060	CONCLUSION The present study may serve as a preliminary reference for the selection of optimum extract in further C. nutans in vivo anti-inflammatory study.
30183131	12	52	dep	C.	2049:2050	arg1	nutans					2052:2057	nutans	2052:2057	nutans	2052:2057	CONCLUSION The present study may serve as a preliminary reference for the selection of optimum extract in further C. nutans in vivo anti-inflammatory study.
30183131	11	53	theme	LC-MS/MS	1784:1791	arg1	quantification					1802:1815	LC-MS/MS targeted quantification	1784:1815	LC-MS/MS targeted quantification	1784:1815	LC-MS/MS targeted quantification further supported sonicated water extract was among the extract that possessed the most abundant C-glycosyl flavones.
30183131	1	54	theme	RAW	167:169	arg1	macrophages					177:187	LPS-IFN-γ activated RAW 264.7 macrophages	147:187	LPS-IFN-γ activated RAW 264.7 macrophages	147:187	f) Lindau water extract against nitric oxide production in LPS-IFN-γ activated RAW 264.7 macrophages.
30183131	4	55	theme	nuclear	679:685	arg1	NMR					707:709	NMR	707:709	NMR	707:709	OBJECTIVE Evaluate the relationship between the chemical composition of C. nutans and its anti-inflammatory properties using nuclear magnetic resonance (NMR) metabolomics approach.
30183131	4	55	theme	nuclear	679:685	arg1	resonance					696:704	nuclear magnetic resonance	679:704	nuclear magnetic resonance (NMR) metabolomics approach	679:732	OBJECTIVE Evaluate the relationship between the chemical composition of C. nutans and its anti-inflammatory properties using nuclear magnetic resonance (NMR) metabolomics approach.
30183131	12	56	from	selection	2009:2017	arg1	study					2085:2089	further C. nutans in vivo anti-inflammatory study	2041:2089	further C. nutans in vivo anti-inflammatory study	2041:2089	CONCLUSION The present study may serve as a preliminary reference for the selection of optimum extract in further C. nutans in vivo anti-inflammatory study.
30183131	5	57	dep	leaves	799:804	arg1	extracted					806:814	extracted	806:814	leaves extracted using five different binary extraction solvent ratio and two extraction methods	799:894	METHODOLOGY The anti-inflammatory effect of C. nutans air-dried leaves extracted using five different binary extraction solvent ratio and two extraction methods was determined based on their nitric oxide (NO) inhibition effect in lipopolysaccharide-interferon-gamma (LPS-IFN-γ) activated RAW 264.7 macrophages.
30183131	4	58	theme	nutans	629:634	arg1	composition					611:621	the chemical composition	598:621	the chemical composition of C. nutans	598:634	OBJECTIVE Evaluate the relationship between the chemical composition of C. nutans and its anti-inflammatory properties using nuclear magnetic resonance (NMR) metabolomics approach.
30183131	4	58	theme	nutans	629:634	arg1	properties					662:671	its anti-inflammatory properties	640:671	its anti-inflammatory properties using nuclear magnetic resonance (NMR) metabolomics approach	640:732	OBJECTIVE Evaluate the relationship between the chemical composition of C. nutans and its anti-inflammatory properties using nuclear magnetic resonance (NMR) metabolomics approach.
30183131	2	59	theme	small	226:230	arg1	INTRODUCTION					190:201	INTRODUCTION	190:201	INTRODUCTION Clinacanthus nutans	190:221	INTRODUCTION Clinacanthus nutans, a small shrub that is native to Southeast Asia, is commonly used in traditional herbal medicine and as a food source.
30183131	2	59	theme	small	226:230	arg1	shrub					232:236	a small shrub	224:236	a small shrub that is native to Southeast Asia	224:269	INTRODUCTION Clinacanthus nutans, a small shrub that is native to Southeast Asia, is commonly used in traditional herbal medicine and as a food source.
30183131	2	59	theme	small	226:230	arg1	native					246:251	native	246:251	native	246:251	INTRODUCTION Clinacanthus nutans, a small shrub that is native to Southeast Asia, is commonly used in traditional herbal medicine and as a food source.
30183131	5	60	theme	extraction	877:886	arg1	methods					888:894	five different binary extraction solvent ratio and two extraction methods	822:894	methods	888:894	METHODOLOGY The anti-inflammatory effect of C. nutans air-dried leaves extracted using five different binary extraction solvent ratio and two extraction methods was determined based on their nitric oxide (NO) inhibition effect in lipopolysaccharide-interferon-gamma (LPS-IFN-γ) activated RAW 264.7 macrophages.
30183131	12	61	dep	in	2059:2060	arg1	vivo					2062:2065	vivo	2062:2065	vivo	2062:2065	CONCLUSION The present study may serve as a preliminary reference for the selection of optimum extract in further C. nutans in vivo anti-inflammatory study.
30183131	1	62	theme	Lindau	91:96	arg1	extract					104:110	f) Lindau water extract	88:110	f) Lindau water extract against nitric oxide production in LPS-IFN-γ activated RAW 264.7 macrophages.	88:188	f) Lindau water extract against nitric oxide production in LPS-IFN-γ activated RAW 264.7 macrophages.
30183131	4	63	theme	metabolomics	712:723	arg1	approach					725:732	nuclear magnetic resonance (NMR) metabolomics approach	679:732	nuclear magnetic resonance (NMR) metabolomics approach	679:732	OBJECTIVE Evaluate the relationship between the chemical composition of C. nutans and its anti-inflammatory properties using nuclear magnetic resonance (NMR) metabolomics approach.
30183131	5	64	theme	inhibition	944:953	arg1	effect					955:960	their nitric oxide (NO) inhibition effect	920:960	their nitric oxide (NO) inhibition effect	920:960	METHODOLOGY The anti-inflammatory effect of C. nutans air-dried leaves extracted using five different binary extraction solvent ratio and two extraction methods was determined based on their nitric oxide (NO) inhibition effect in lipopolysaccharide-interferon-gamma (LPS-IFN-γ) activated RAW 264.7 macrophages.
30183131	5	65	theme	nitric	926:931	arg1	oxide					933:937	their nitric oxide	920:937	their nitric oxide (NO) inhibition effect	920:960	METHODOLOGY The anti-inflammatory effect of C. nutans air-dried leaves extracted using five different binary extraction solvent ratio and two extraction methods was determined based on their nitric oxide (NO) inhibition effect in lipopolysaccharide-interferon-gamma (LPS-IFN-γ) activated RAW 264.7 macrophages.
30183131	5	65	theme	nitric	926:931	arg1	NO					940:941	NO	940:941	NO	940:941	METHODOLOGY The anti-inflammatory effect of C. nutans air-dried leaves extracted using five different binary extraction solvent ratio and two extraction methods was determined based on their nitric oxide (NO) inhibition effect in lipopolysaccharide-interferon-gamma (LPS-IFN-γ) activated RAW 264.7 macrophages.
30183131	3	66	theme	different	448:456	arg1	ratio					484:488	different binary extraction solvent ratio and extraction methods	448:511	ratio	484:488	Its anti-inflammation properties is influenced by the metabolites composition, which can be determined by different binary extraction solvent ratio and extraction methods used during plant post-harvesting stage.
30183131	0	67	theme	Clinacanthus	61:72	arg1	nutans					74:79	Clinacanthus nutans	61:79	Clinacanthus nutans (Burm	61:85	1 H-NMR metabolomics for evaluating the protective effect of Clinacanthus nutans (Burm.
30183131	10	68	contain	containing	1621:1630	arg2	compounds					1662:1670	sulphur containing compounds	1643:1670	sulphur containing compounds	1643:1670	A partial least square (PLS) biplot suggested that sulphur containing glucoside, sulphur containing compounds, phytosterols, triterpenoids, flavones and some organic and amino acids were among the potential NO inhibitors.
30183131	10	68	contain	containing	1621:1630	arg2	glucoside					1632:1640	glucoside	1632:1640	glucoside	1632:1640	A partial least square (PLS) biplot suggested that sulphur containing glucoside, sulphur containing compounds, phytosterols, triterpenoids, flavones and some organic and amino acids were among the potential NO inhibitors.
30183131	10	68	contain	containing	1621:1630	arg2	phytosterols					1673:1684	phytosterols	1673:1684	phytosterols	1673:1684	A partial least square (PLS) biplot suggested that sulphur containing glucoside, sulphur containing compounds, phytosterols, triterpenoids, flavones and some organic and amino acids were among the potential NO inhibitors.
30183131	10	68	contain	containing	1621:1630	arg2	triterpenoids					1687:1699	triterpenoids	1687:1699	triterpenoids	1687:1699	A partial least square (PLS) biplot suggested that sulphur containing glucoside, sulphur containing compounds, phytosterols, triterpenoids, flavones and some organic and amino acids were among the potential NO inhibitors.
30183131	10	68	contain	containing	1621:1630	arg1	sulphur					1613:1619	sulphur	1613:1619	sulphur containing glucoside, sulphur containing compounds, phytosterols, triterpenoids, flavones and some organic and amino acids	1613:1742	A partial least square (PLS) biplot suggested that sulphur containing glucoside, sulphur containing compounds, phytosterols, triterpenoids, flavones and some organic and amino acids were among the potential NO inhibitors.
30183131	10	68	contain	containing	1621:1630	arg2	flavones					1702:1709	flavones	1702:1709	flavones	1702:1709	A partial least square (PLS) biplot suggested that sulphur containing glucoside, sulphur containing compounds, phytosterols, triterpenoids, flavones and some organic and amino acids were among the potential NO inhibitors.
30183131	10	68	contain	containing	1621:1630	arg2	acids					1738:1742	some organic and amino acids	1715:1742	acids	1738:1742	A partial least square (PLS) biplot suggested that sulphur containing glucoside, sulphur containing compounds, phytosterols, triterpenoids, flavones and some organic and amino acids were among the potential NO inhibitors.
30183131	6	69	theme	extract	1071:1077	arg1	bioactivity					1079:1089	extract bioactivity and metabolite profiles	1071:1113	bioactivity	1079:1089	The relationship between extract bioactivity and metabolite profiles and quantifications were established using 1 H-NMR metabolomics and liquid chromatography-tandem mass spectrometry (LC-MS/MS).
30183131	5	70	dep	METHODOLOGY	735:745	arg1	leaves					799:804	leaves	799:804	leaves extracted using five different binary extraction solvent ratio and two extraction methods	799:894	METHODOLOGY The anti-inflammatory effect of C. nutans air-dried leaves extracted using five different binary extraction solvent ratio and two extraction methods was determined based on their nitric oxide (NO) inhibition effect in lipopolysaccharide-interferon-gamma (LPS-IFN-γ) activated RAW 264.7 macrophages.
30183131	4	71	theme	resonance	696:704	arg1	approach					725:732	nuclear magnetic resonance (NMR) metabolomics approach	679:732	nuclear magnetic resonance (NMR) metabolomics approach	679:732	OBJECTIVE Evaluate the relationship between the chemical composition of C. nutans and its anti-inflammatory properties using nuclear magnetic resonance (NMR) metabolomics approach.
30183131	5	72	theme	air-dried	789:797	arg1	effect					769:774	The anti-inflammatory effect	747:774	The anti-inflammatory effect of C. nutans air-dried	747:797	METHODOLOGY The anti-inflammatory effect of C. nutans air-dried leaves extracted using five different binary extraction solvent ratio and two extraction methods was determined based on their nitric oxide (NO) inhibition effect in lipopolysaccharide-interferon-gamma (LPS-IFN-γ) activated RAW 264.7 macrophages.
30183131	5	73	theme	oxide	933:937	arg1	effect					955:960	their nitric oxide (NO) inhibition effect	920:960	their nitric oxide (NO) inhibition effect	920:960	METHODOLOGY The anti-inflammatory effect of C. nutans air-dried leaves extracted using five different binary extraction solvent ratio and two extraction methods was determined based on their nitric oxide (NO) inhibition effect in lipopolysaccharide-interferon-gamma (LPS-IFN-γ) activated RAW 264.7 macrophages.
30183131	10	74	theme	partial	1564:1570	arg1	biplot					1591:1596	A partial least square (PLS) biplot	1562:1596	A partial least square (PLS) biplot	1562:1596	A partial least square (PLS) biplot suggested that sulphur containing glucoside, sulphur containing compounds, phytosterols, triterpenoids, flavones and some organic and amino acids were among the potential NO inhibitors.
30183131	12	75	theme	anti-inflammatory	2067:2083	arg1	study					2085:2089	further C. nutans in vivo anti-inflammatory study	2041:2089	further C. nutans in vivo anti-inflammatory study	2041:2089	CONCLUSION The present study may serve as a preliminary reference for the selection of optimum extract in further C. nutans in vivo anti-inflammatory study.
30183131	2	76	theme	traditional	292:302	arg1	medicine					311:318	traditional herbal medicine	292:318	traditional herbal medicine	292:318	INTRODUCTION Clinacanthus nutans, a small shrub that is native to Southeast Asia, is commonly used in traditional herbal medicine and as a food source.
30183131	5	77	theme	RAW	1023:1025	arg1	macrophages					1033:1043	lipopolysaccharide-interferon-gamma (LPS-IFN-γ) activated RAW 264.7 macrophages	965:1043	lipopolysaccharide-interferon-gamma (LPS-IFN-γ) activated RAW 264.7 macrophages	965:1043	METHODOLOGY The anti-inflammatory effect of C. nutans air-dried leaves extracted using five different binary extraction solvent ratio and two extraction methods was determined based on their nitric oxide (NO) inhibition effect in lipopolysaccharide-interferon-gamma (LPS-IFN-γ) activated RAW 264.7 macrophages.
30183131	11	78	theme	abundant	1905:1912	arg1	flavones					1925:1932	the most abundant C-glycosyl flavones	1896:1932	the most abundant C-glycosyl flavones	1896:1932	LC-MS/MS targeted quantification further supported sonicated water extract was among the extract that possessed the most abundant C-glycosyl flavones.
30183131	12	79	dep	CONCLUSION	1935:1944	arg1	serve					1968:1972	serve	1968:1972	may serve as a preliminary reference for the selection of optimum extract in further C. nutans in vivo anti-inflammatory study	1964:2089	CONCLUSION The present study may serve as a preliminary reference for the selection of optimum extract in further C. nutans in vivo anti-inflammatory study.
30183131	10	80	theme	NO	1769:1770	arg1	inhibitors					1772:1781	the potential NO inhibitors	1755:1781	the potential NO inhibitors	1755:1781	A partial least square (PLS) biplot suggested that sulphur containing glucoside, sulphur containing compounds, phytosterols, triterpenoids, flavones and some organic and amino acids were among the potential NO inhibitors.
30183131	5	81	theme	binary	837:842	arg1	ratio					863:867	five different binary extraction solvent ratio and two extraction methods	822:894	ratio	863:867	METHODOLOGY The anti-inflammatory effect of C. nutans air-dried leaves extracted using five different binary extraction solvent ratio and two extraction methods was determined based on their nitric oxide (NO) inhibition effect in lipopolysaccharide-interferon-gamma (LPS-IFN-γ) activated RAW 264.7 macrophages.
30183131	12	82	theme	C.	2049:2050	arg1	study					2085:2089	further C. nutans in vivo anti-inflammatory study	2041:2089	further C. nutans in vivo anti-inflammatory study	2041:2089	CONCLUSION The present study may serve as a preliminary reference for the selection of optimum extract in further C. nutans in vivo anti-inflammatory study.
30183131	10	83	theme	square	1578:1583	arg1	biplot					1591:1596	A partial least square (PLS) biplot	1562:1596	A partial least square (PLS) biplot	1562:1596	A partial least square (PLS) biplot suggested that sulphur containing glucoside, sulphur containing compounds, phytosterols, triterpenoids, flavones and some organic and amino acids were among the potential NO inhibitors.
30183131	5	84	theme	solvent	855:861	arg1	ratio					863:867	five different binary extraction solvent ratio and two extraction methods	822:894	ratio	863:867	METHODOLOGY The anti-inflammatory effect of C. nutans air-dried leaves extracted using five different binary extraction solvent ratio and two extraction methods was determined based on their nitric oxide (NO) inhibition effect in lipopolysaccharide-interferon-gamma (LPS-IFN-γ) activated RAW 264.7 macrophages.
30183131	1	85	theme	f	88:88	arg1	extract					104:110	f) Lindau water extract	88:110	f) Lindau water extract against nitric oxide production in LPS-IFN-γ activated RAW 264.7 macrophages.	88:188	f) Lindau water extract against nitric oxide production in LPS-IFN-γ activated RAW 264.7 macrophages.
30183131	8	86	dep	air-dried	1377:1385	arg1	leaves					1387:1392	leaves	1387:1392	leaves	1387:1392	RESULTS Water and sonication prepared air-dried leaves possessed the highest NO inhibition activity (IC50 = 190.43 ± 12.26 μg/mL, P < 0.05).
30183131	11	87	theme	sonicated	1835:1843	arg1	extract					1851:1857	sonicated water extract	1835:1857	sonicated water extract was among the extract that possessed the most abundant C-glycosyl flavones	1835:1932	LC-MS/MS targeted quantification further supported sonicated water extract was among the extract that possessed the most abundant C-glycosyl flavones.
30183131	12	88	theme	optimum	2022:2028	arg1	extract					2030:2036	optimum extract	2022:2036	optimum extract	2022:2036	CONCLUSION The present study may serve as a preliminary reference for the selection of optimum extract in further C. nutans in vivo anti-inflammatory study.
30183131	12	89	theme	preliminary	1979:1989	arg1	reference					1991:1999	a preliminary reference	1977:1999	a preliminary reference for the selection of optimum extract in further C. nutans in vivo anti-inflammatory study	1977:2089	CONCLUSION The present study may serve as a preliminary reference for the selection of optimum extract in further C. nutans in vivo anti-inflammatory study.
30183131	12	89	theme	preliminary	1979:1989	arg1	study					1958:1962	The present study	1946:1962	The present study	1946:1962	CONCLUSION The present study may serve as a preliminary reference for the selection of optimum extract in further C. nutans in vivo anti-inflammatory study.
30183131	2	90	theme	food	329:332	arg1	INTRODUCTION					190:201	INTRODUCTION	190:201	INTRODUCTION Clinacanthus nutans	190:221	INTRODUCTION Clinacanthus nutans, a small shrub that is native to Southeast Asia, is commonly used in traditional herbal medicine and as a food source.
30183131	2	90	theme	food	329:332	arg1	source					334:339	a food source	327:339	a food source	327:339	INTRODUCTION Clinacanthus nutans, a small shrub that is native to Southeast Asia, is commonly used in traditional herbal medicine and as a food source.
30183131	2	91	theme	Clinacanthus	203:214	arg1	native					246:251	native	246:251	native	246:251	INTRODUCTION Clinacanthus nutans, a small shrub that is native to Southeast Asia, is commonly used in traditional herbal medicine and as a food source.
30183131	2	91	theme	Clinacanthus	203:214	arg1	INTRODUCTION					190:201	INTRODUCTION	190:201	INTRODUCTION Clinacanthus nutans	190:221	INTRODUCTION Clinacanthus nutans, a small shrub that is native to Southeast Asia, is commonly used in traditional herbal medicine and as a food source.
30183131	2	91	theme	Clinacanthus	203:214	arg1	shrub					232:236	a small shrub	224:236	a small shrub that is native to Southeast Asia	224:269	INTRODUCTION Clinacanthus nutans, a small shrub that is native to Southeast Asia, is commonly used in traditional herbal medicine and as a food source.
30183131	2	91	theme	Clinacanthus	203:214	arg1	source					334:339	a food source	327:339	a food source	327:339	INTRODUCTION Clinacanthus nutans, a small shrub that is native to Southeast Asia, is commonly used in traditional herbal medicine and as a food source.
30183131	3	92	theme	solvent	476:482	arg1	ratio					484:488	different binary extraction solvent ratio and extraction methods	448:511	ratio	484:488	Its anti-inflammation properties is influenced by the metabolites composition, which can be determined by different binary extraction solvent ratio and extraction methods used during plant post-harvesting stage.
30183131	6	93	theme	mass	1212:1215	arg1	LC-MS/MS					1231:1238	LC-MS/MS	1231:1238	LC-MS/MS	1231:1238	The relationship between extract bioactivity and metabolite profiles and quantifications were established using 1 H-NMR metabolomics and liquid chromatography-tandem mass spectrometry (LC-MS/MS).
30183131	6	93	theme	mass	1212:1215	arg1	spectrometry					1217:1228	liquid chromatography-tandem mass spectrometry	1183:1228	liquid chromatography-tandem mass spectrometry (LC-MS/MS)	1183:1239	The relationship between extract bioactivity and metabolite profiles and quantifications were established using 1 H-NMR metabolomics and liquid chromatography-tandem mass spectrometry (LC-MS/MS).
30183131	3	94	theme	binary	458:463	arg1	ratio					484:488	different binary extraction solvent ratio and extraction methods	448:511	ratio	484:488	Its anti-inflammation properties is influenced by the metabolites composition, which can be determined by different binary extraction solvent ratio and extraction methods used during plant post-harvesting stage.
30183131	6	95	theme	liquid	1183:1188	arg1	LC-MS/MS					1231:1238	LC-MS/MS	1231:1238	LC-MS/MS	1231:1238	The relationship between extract bioactivity and metabolite profiles and quantifications were established using 1 H-NMR metabolomics and liquid chromatography-tandem mass spectrometry (LC-MS/MS).
30183131	6	95	theme	liquid	1183:1188	arg1	spectrometry					1217:1228	liquid chromatography-tandem mass spectrometry	1183:1228	liquid chromatography-tandem mass spectrometry (LC-MS/MS)	1183:1239	The relationship between extract bioactivity and metabolite profiles and quantifications were established using 1 H-NMR metabolomics and liquid chromatography-tandem mass spectrometry (LC-MS/MS).
30183131	2	96	dep	Clinacanthus	203:214	arg1	nutans					216:221	nutans	216:221	nutans	216:221	INTRODUCTION Clinacanthus nutans, a small shrub that is native to Southeast Asia, is commonly used in traditional herbal medicine and as a food source.
30183131	0	97	theme	H-NMR	2:6	arg1	metabolomics					8:19	1 H-NMR metabolomics	0:19	1 H-NMR metabolomics for evaluating the protective effect of Clinacanthus nutans (Burm.	0:86	1 H-NMR metabolomics for evaluating the protective effect of Clinacanthus nutans (Burm.
30600007	7	0	theme	pharmacological	1330:1344	arg1	actions					1346:1352	the pharmacological actions	1326:1352	the pharmacological actions of AERP on the protection from CIALI in mice	1326:1397	These findings not only provide novel insights into the pharmacological actions of AERP on the protection from CIALI in mice, but they also demonstrate that determining SCFA profiles by targeted GC-MS metabolomics is an effective technique to investigate the molecular mechanisms of the effects of plant polysaccharides on intestinal flora metabolism.
30600007	1	1	theme	Smith	391:395	arg1	degradations					397:408	Smith degradations	391:408	Smith degradations	391:408	The structural characteristics of the polysaccharides from Aralia elata root barks (AERP) were systematically investigated by FT-IR, HPSEC-ELSD and colorimetric methods as well as by GCMS based monosaccharide compositions, Smith degradations, and methylation analysis.
30600007	5	2	theme	ELISA	1078:1082	arg1	assays					1084:1089	ELISA assays	1078:1089	ELISA assays	1078:1089	Studies to evaluate hepatoprotective potentials of AERP showed that they had potent hepatoprotective effects in vivo in carbon tetrachloride-induced acute liver injury (CIALI) in mice by histopathological evaluation, biochemical examinations and ELISA assays.
30600007	7	3	theme	novel	1306:1310	arg1	insights					1312:1319	novel insights	1306:1319	novel insights into the pharmacological actions of AERP on the protection from CIALI in mice	1306:1397	These findings not only provide novel insights into the pharmacological actions of AERP on the protection from CIALI in mice, but they also demonstrate that determining SCFA profiles by targeted GC-MS metabolomics is an effective technique to investigate the molecular mechanisms of the effects of plant polysaccharides on intestinal flora metabolism.
30600007	4	4	theme	glycosidic	718:727	arg1	linkages					729:736	The abundant glycosidic linkages	705:736	The abundant glycosidic linkages of Man, Ara, Gal, and Rha	705:762	The abundant glycosidic linkages of Man, Ara, Gal, and Rha were observed as more than 90% of all the monosaccharides detected.
30600007	4	4	theme	glycosidic	718:727	arg1	%					793:793	more than 90%	781:793	more than 90% of all the monosaccharides detected	781:829	The abundant glycosidic linkages of Man, Ara, Gal, and Rha were observed as more than 90% of all the monosaccharides detected.
30600007	4	4	theme	glycosidic	718:727	arg1	monosaccharides					806:820	all the monosaccharides	798:820	all the monosaccharides detected	798:829	The abundant glycosidic linkages of Man, Ara, Gal, and Rha were observed as more than 90% of all the monosaccharides detected.
30600007	7	5	from	effects	1561:1567	arg1	metabolism					1614:1623	intestinal flora metabolism	1597:1623	intestinal flora metabolism	1597:1623	These findings not only provide novel insights into the pharmacological actions of AERP on the protection from CIALI in mice, but they also demonstrate that determining SCFA profiles by targeted GC-MS metabolomics is an effective technique to investigate the molecular mechanisms of the effects of plant polysaccharides on intestinal flora metabolism.
30600007	3	6	theme	sulfate	662:668	arg1	number					652:657	a small number	644:657	a small number of sulfate (3.38%) and acetyl (4.87%) groups	644:702	AERP was composed of Ara, Rha, GlcA, Man, Glc, and Gal in a molar ratio of 22.2: 10.3: 8.1: 32.7: 5.7: 21.2 along with a small number of sulfate (3.38%) and acetyl (4.87%) groups.
30600007	1	7	theme	polysaccharides	206:220	arg1	characteristics					183:197	The structural characteristics	168:197	The structural characteristics of the polysaccharides from Aralia elata root barks (AERP)	168:256	The structural characteristics of the polysaccharides from Aralia elata root barks (AERP) were systematically investigated by FT-IR, HPSEC-ELSD and colorimetric methods as well as by GCMS based monosaccharide compositions, Smith degradations, and methylation analysis.
30600007	6	8	theme	common	1180:1185	arg1	SCFAs					1212:1216	SCFAs	1212:1216	SCFAs	1212:1216	GCMS was further used to determine the effects of AERP on the chemical profiles of nine common short-chain fatty acids (SCFAs) produced by intestinal flora metabolism in CIALI mice.
30600007	6	8	theme	common	1180:1185	arg1	acids					1205:1209	nine common short-chain fatty acids	1175:1209	nine common short-chain fatty acids (SCFAs) produced by intestinal flora metabolism in CIALI mice	1175:1271	GCMS was further used to determine the effects of AERP on the chemical profiles of nine common short-chain fatty acids (SCFAs) produced by intestinal flora metabolism in CIALI mice.
30600007	5	9	theme	potent	909:914	arg1	effects					933:939	potent hepatoprotective effects	909:939	potent hepatoprotective effects	909:939	Studies to evaluate hepatoprotective potentials of AERP showed that they had potent hepatoprotective effects in vivo in carbon tetrachloride-induced acute liver injury (CIALI) in mice by histopathological evaluation, biochemical examinations and ELISA assays.
30600007	1	10	theme	Aralia	227:232	arg1	AERP					252:255	AERP	252:255	AERP	252:255	The structural characteristics of the polysaccharides from Aralia elata root barks (AERP) were systematically investigated by FT-IR, HPSEC-ELSD and colorimetric methods as well as by GCMS based monosaccharide compositions, Smith degradations, and methylation analysis.
30600007	1	10	theme	Aralia	227:232	arg1	barks					245:249	Aralia elata root barks	227:249	Aralia elata root barks (AERP)	227:256	The structural characteristics of the polysaccharides from Aralia elata root barks (AERP) were systematically investigated by FT-IR, HPSEC-ELSD and colorimetric methods as well as by GCMS based monosaccharide compositions, Smith degradations, and methylation analysis.
30600007	7	11	theme	GC-MS	1469:1473	arg1	metabolomics					1475:1486	targeted GC-MS metabolomics	1460:1486	targeted GC-MS metabolomics	1460:1486	These findings not only provide novel insights into the pharmacological actions of AERP on the protection from CIALI in mice, but they also demonstrate that determining SCFA profiles by targeted GC-MS metabolomics is an effective technique to investigate the molecular mechanisms of the effects of plant polysaccharides on intestinal flora metabolism.
30600007	0	12	theme	intestinal	139:148	arg1	metabolism					156:165	intestinal flora metabolism	139:165	intestinal flora metabolism	139:165	Structural characteristics and hepatoprotective potential of Aralia elata root bark polysaccharides and their effects on SCFAs produced by intestinal flora metabolism.
30600007	1	13	theme	root	240:243	arg1	AERP					252:255	AERP	252:255	AERP	252:255	The structural characteristics of the polysaccharides from Aralia elata root barks (AERP) were systematically investigated by FT-IR, HPSEC-ELSD and colorimetric methods as well as by GCMS based monosaccharide compositions, Smith degradations, and methylation analysis.
30600007	1	13	theme	root	240:243	arg1	barks					245:249	Aralia elata root barks	227:249	Aralia elata root barks (AERP)	227:256	The structural characteristics of the polysaccharides from Aralia elata root barks (AERP) were systematically investigated by FT-IR, HPSEC-ELSD and colorimetric methods as well as by GCMS based monosaccharide compositions, Smith degradations, and methylation analysis.
30600007	6	14	theme	chemical	1154:1161	arg1	profiles					1163:1170	the chemical profiles	1150:1170	the chemical profiles of nine common short-chain fatty acids (SCFAs) produced by intestinal flora metabolism in CIALI mice	1150:1271	GCMS was further used to determine the effects of AERP on the chemical profiles of nine common short-chain fatty acids (SCFAs) produced by intestinal flora metabolism in CIALI mice.
30600007	6	15	from	metabolism	1248:1257	arg1	mice					1268:1271	CIALI mice	1262:1271	CIALI mice	1262:1271	GCMS was further used to determine the effects of AERP on the chemical profiles of nine common short-chain fatty acids (SCFAs) produced by intestinal flora metabolism in CIALI mice.
30600007	7	16	theme	SCFA	1443:1446	arg1	profiles					1448:1455	SCFA profiles	1443:1455	SCFA profiles	1443:1455	These findings not only provide novel insights into the pharmacological actions of AERP on the protection from CIALI in mice, but they also demonstrate that determining SCFA profiles by targeted GC-MS metabolomics is an effective technique to investigate the molecular mechanisms of the effects of plant polysaccharides on intestinal flora metabolism.
30600007	4	17	theme	Rha	760:762	arg1	linkages					729:736	The abundant glycosidic linkages	705:736	The abundant glycosidic linkages of Man, Ara, Gal, and Rha	705:762	The abundant glycosidic linkages of Man, Ara, Gal, and Rha were observed as more than 90% of all the monosaccharides detected.
30600007	4	17	theme	Rha	760:762	arg1	%					793:793	more than 90%	781:793	more than 90% of all the monosaccharides detected	781:829	The abundant glycosidic linkages of Man, Ara, Gal, and Rha were observed as more than 90% of all the monosaccharides detected.
30600007	4	17	theme	Rha	760:762	arg1	monosaccharides					806:820	all the monosaccharides	798:820	all the monosaccharides detected	798:829	The abundant glycosidic linkages of Man, Ara, Gal, and Rha were observed as more than 90% of all the monosaccharides detected.
30600007	7	18	dep	findings	1280:1287	arg1	only					1293:1296	only	1293:1296	only	1293:1296	These findings not only provide novel insights into the pharmacological actions of AERP on the protection from CIALI in mice, but they also demonstrate that determining SCFA profiles by targeted GC-MS metabolomics is an effective technique to investigate the molecular mechanisms of the effects of plant polysaccharides on intestinal flora metabolism.
30600007	7	19	theme	plant	1572:1576	arg1	polysaccharides					1578:1592	plant polysaccharides	1572:1592	plant polysaccharides	1572:1592	These findings not only provide novel insights into the pharmacological actions of AERP on the protection from CIALI in mice, but they also demonstrate that determining SCFA profiles by targeted GC-MS metabolomics is an effective technique to investigate the molecular mechanisms of the effects of plant polysaccharides on intestinal flora metabolism.
30600007	6	20	used	used	1109:1112	arg2	GCMS					1092:1095	GCMS	1092:1095	GCMS	1092:1095	GCMS was further used to determine the effects of AERP on the chemical profiles of nine common short-chain fatty acids (SCFAs) produced by intestinal flora metabolism in CIALI mice.
30600007	3	21	theme	acetyl	682:687	arg1	groups					697:702	sulfate (3.38%) and acetyl (4.87%) groups	662:702	groups	697:702	AERP was composed of Ara, Rha, GlcA, Man, Glc, and Gal in a molar ratio of 22.2: 10.3: 8.1: 32.7: 5.7: 21.2 along with a small number of sulfate (3.38%) and acetyl (4.87%) groups.
30600007	3	21	theme	acetyl	682:687	arg1	%					694:694	4.87%	690:694	4.87%	690:694	AERP was composed of Ara, Rha, GlcA, Man, Glc, and Gal in a molar ratio of 22.2: 10.3: 8.1: 32.7: 5.7: 21.2 along with a small number of sulfate (3.38%) and acetyl (4.87%) groups.
30600007	0	22	theme	root	74:77	arg1	polysaccharides					84:98	Aralia elata root bark polysaccharides	61:98	Aralia elata root bark polysaccharides	61:98	Structural characteristics and hepatoprotective potential of Aralia elata root bark polysaccharides and their effects on SCFAs produced by intestinal flora metabolism.
30600007	5	23	theme	acute	981:985	arg1	CIALI					1001:1005	CIALI	1001:1005	CIALI	1001:1005	Studies to evaluate hepatoprotective potentials of AERP showed that they had potent hepatoprotective effects in vivo in carbon tetrachloride-induced acute liver injury (CIALI) in mice by histopathological evaluation, biochemical examinations and ELISA assays.
30600007	5	23	theme	acute	981:985	arg1	injury					993:998	carbon tetrachloride-induced acute liver injury	952:998	carbon tetrachloride-induced acute liver injury (CIALI) in mice by histopathological evaluation, biochemical examinations and ELISA assays	952:1089	Studies to evaluate hepatoprotective potentials of AERP showed that they had potent hepatoprotective effects in vivo in carbon tetrachloride-induced acute liver injury (CIALI) in mice by histopathological evaluation, biochemical examinations and ELISA assays.
30600007	1	24	from	characteristics	183:197	arg1	AERP					252:255	AERP	252:255	AERP	252:255	The structural characteristics of the polysaccharides from Aralia elata root barks (AERP) were systematically investigated by FT-IR, HPSEC-ELSD and colorimetric methods as well as by GCMS based monosaccharide compositions, Smith degradations, and methylation analysis.
30600007	1	24	from	characteristics	183:197	arg1	barks					245:249	Aralia elata root barks	227:249	Aralia elata root barks (AERP)	227:256	The structural characteristics of the polysaccharides from Aralia elata root barks (AERP) were systematically investigated by FT-IR, HPSEC-ELSD and colorimetric methods as well as by GCMS based monosaccharide compositions, Smith degradations, and methylation analysis.
30600007	7	25	theme	effects	1561:1567	arg1	mechanisms					1543:1552	the molecular mechanisms	1529:1552	the molecular mechanisms of the effects of plant polysaccharides on intestinal flora metabolism	1529:1623	These findings not only provide novel insights into the pharmacological actions of AERP on the protection from CIALI in mice, but they also demonstrate that determining SCFA profiles by targeted GC-MS metabolomics is an effective technique to investigate the molecular mechanisms of the effects of plant polysaccharides on intestinal flora metabolism.
30600007	0	26	theme	polysaccharides	84:98	arg1	effects					110:116	their effects	104:116	their effects on SCFAs	104:125	Structural characteristics and hepatoprotective potential of Aralia elata root bark polysaccharides and their effects on SCFAs produced by intestinal flora metabolism.
30600007	0	26	theme	polysaccharides	84:98	arg1	characteristics					11:25	Structural characteristics	0:25	Structural characteristics	0:25	Structural characteristics and hepatoprotective potential of Aralia elata root bark polysaccharides and their effects on SCFAs produced by intestinal flora metabolism.
30600007	0	26	theme	polysaccharides	84:98	arg1	potential					48:56	hepatoprotective potential	31:56	hepatoprotective potential	31:56	Structural characteristics and hepatoprotective potential of Aralia elata root bark polysaccharides and their effects on SCFAs produced by intestinal flora metabolism.
30600007	1	27	theme	structural	172:181	arg1	characteristics					183:197	The structural characteristics	168:197	The structural characteristics of the polysaccharides from Aralia elata root barks (AERP)	168:256	The structural characteristics of the polysaccharides from Aralia elata root barks (AERP) were systematically investigated by FT-IR, HPSEC-ELSD and colorimetric methods as well as by GCMS based monosaccharide compositions, Smith degradations, and methylation analysis.
30600007	1	28	theme	monosaccharide	362:375	arg1	compositions					377:388	monosaccharide compositions	362:388	monosaccharide compositions	362:388	The structural characteristics of the polysaccharides from Aralia elata root barks (AERP) were systematically investigated by FT-IR, HPSEC-ELSD and colorimetric methods as well as by GCMS based monosaccharide compositions, Smith degradations, and methylation analysis.
30600007	7	29	theme	molecular	1533:1541	arg1	mechanisms					1543:1552	the molecular mechanisms	1529:1552	the molecular mechanisms of the effects of plant polysaccharides on intestinal flora metabolism	1529:1623	These findings not only provide novel insights into the pharmacological actions of AERP on the protection from CIALI in mice, but they also demonstrate that determining SCFA profiles by targeted GC-MS metabolomics is an effective technique to investigate the molecular mechanisms of the effects of plant polysaccharides on intestinal flora metabolism.
30600007	6	30	theme	fatty	1199:1203	arg1	SCFAs					1212:1216	SCFAs	1212:1216	SCFAs	1212:1216	GCMS was further used to determine the effects of AERP on the chemical profiles of nine common short-chain fatty acids (SCFAs) produced by intestinal flora metabolism in CIALI mice.
30600007	6	30	theme	fatty	1199:1203	arg1	acids					1205:1209	nine common short-chain fatty acids	1175:1209	nine common short-chain fatty acids (SCFAs) produced by intestinal flora metabolism in CIALI mice	1175:1271	GCMS was further used to determine the effects of AERP on the chemical profiles of nine common short-chain fatty acids (SCFAs) produced by intestinal flora metabolism in CIALI mice.
30600007	5	31	theme	carbon	952:957	arg1	CIALI					1001:1005	CIALI	1001:1005	CIALI	1001:1005	Studies to evaluate hepatoprotective potentials of AERP showed that they had potent hepatoprotective effects in vivo in carbon tetrachloride-induced acute liver injury (CIALI) in mice by histopathological evaluation, biochemical examinations and ELISA assays.
30600007	5	31	theme	carbon	952:957	arg1	injury					993:998	carbon tetrachloride-induced acute liver injury	952:998	carbon tetrachloride-induced acute liver injury (CIALI) in mice by histopathological evaluation, biochemical examinations and ELISA assays	952:1089	Studies to evaluate hepatoprotective potentials of AERP showed that they had potent hepatoprotective effects in vivo in carbon tetrachloride-induced acute liver injury (CIALI) in mice by histopathological evaluation, biochemical examinations and ELISA assays.
30600007	2	32	dep	showed	448:453	arg1	93.9 kDa					515:522	93.9 kDa	515:522	93.9 kDa	515:522	The result showed average molecular weights of AERP were between 42.7 kDa and 93.9 kDa.
30600007	2	32	dep	showed	448:453	arg1	42.7 kDa					502:509	42.7 kDa	502:509	42.7 kDa	502:509	The result showed average molecular weights of AERP were between 42.7 kDa and 93.9 kDa.
30600007	0	33	from	potential	48:56	arg1	SCFAs					121:125	SCFAs	121:125	SCFAs	121:125	Structural characteristics and hepatoprotective potential of Aralia elata root bark polysaccharides and their effects on SCFAs produced by intestinal flora metabolism.
30600007	0	34	theme	bark	79:82	arg1	polysaccharides					84:98	Aralia elata root bark polysaccharides	61:98	Aralia elata root bark polysaccharides	61:98	Structural characteristics and hepatoprotective potential of Aralia elata root bark polysaccharides and their effects on SCFAs produced by intestinal flora metabolism.
30600007	0	35	theme	Structural	0:9	arg1	characteristics					11:25	Structural characteristics	0:25	Structural characteristics	0:25	Structural characteristics and hepatoprotective potential of Aralia elata root bark polysaccharides and their effects on SCFAs produced by intestinal flora metabolism.
30600007	2	36	theme	average	455:461	arg1	weights					473:479	average molecular weights	455:479	average molecular weights of AERP	455:487	The result showed average molecular weights of AERP were between 42.7 kDa and 93.9 kDa.
30600007	5	37	contain	had	905:907	arg1	they					900:903	they	900:903	they	900:903	Studies to evaluate hepatoprotective potentials of AERP showed that they had potent hepatoprotective effects in vivo in carbon tetrachloride-induced acute liver injury (CIALI) in mice by histopathological evaluation, biochemical examinations and ELISA assays.
30600007	5	37	contain	had	905:907	arg2	effects					933:939	potent hepatoprotective effects	909:939	potent hepatoprotective effects	909:939	Studies to evaluate hepatoprotective potentials of AERP showed that they had potent hepatoprotective effects in vivo in carbon tetrachloride-induced acute liver injury (CIALI) in mice by histopathological evaluation, biochemical examinations and ELISA assays.
30600007	3	38	from	Gal	576:578	arg1	ratio					591:595	a molar ratio	583:595	a molar ratio of 22.2: 10.3: 8.1: 32.7: 5.7: 21.2	583:631	AERP was composed of Ara, Rha, GlcA, Man, Glc, and Gal in a molar ratio of 22.2: 10.3: 8.1: 32.7: 5.7: 21.2 along with a small number of sulfate (3.38%) and acetyl (4.87%) groups.
30600007	1	39	theme	colorimetric	316:327	arg1	methods					329:335	colorimetric methods	316:335	colorimetric methods	316:335	The structural characteristics of the polysaccharides from Aralia elata root barks (AERP) were systematically investigated by FT-IR, HPSEC-ELSD and colorimetric methods as well as by GCMS based monosaccharide compositions, Smith degradations, and methylation analysis.
30600007	2	40	theme	AERP	484:487	arg1	weights					473:479	average molecular weights	455:479	average molecular weights of AERP	455:487	The result showed average molecular weights of AERP were between 42.7 kDa and 93.9 kDa.
30600007	6	41	theme	flora	1242:1246	arg1	metabolism					1248:1257	intestinal flora metabolism	1231:1257	intestinal flora metabolism in CIALI mice	1231:1271	GCMS was further used to determine the effects of AERP on the chemical profiles of nine common short-chain fatty acids (SCFAs) produced by intestinal flora metabolism in CIALI mice.
30600007	7	42	theme	AERP	1357:1360	arg1	actions					1346:1352	the pharmacological actions	1326:1352	the pharmacological actions of AERP on the protection from CIALI in mice	1326:1397	These findings not only provide novel insights into the pharmacological actions of AERP on the protection from CIALI in mice, but they also demonstrate that determining SCFA profiles by targeted GC-MS metabolomics is an effective technique to investigate the molecular mechanisms of the effects of plant polysaccharides on intestinal flora metabolism.
30600007	5	43	theme	AERP	883:886	arg1	potentials					869:878	hepatoprotective potentials	852:878	hepatoprotective potentials of AERP	852:886	Studies to evaluate hepatoprotective potentials of AERP showed that they had potent hepatoprotective effects in vivo in carbon tetrachloride-induced acute liver injury (CIALI) in mice by histopathological evaluation, biochemical examinations and ELISA assays.
30600007	7	44	from	metabolism	1614:1623	arg1	mechanisms					1543:1552	the molecular mechanisms	1529:1552	the molecular mechanisms of the effects of plant polysaccharides on intestinal flora metabolism	1529:1623	These findings not only provide novel insights into the pharmacological actions of AERP on the protection from CIALI in mice, but they also demonstrate that determining SCFA profiles by targeted GC-MS metabolomics is an effective technique to investigate the molecular mechanisms of the effects of plant polysaccharides on intestinal flora metabolism.
30600007	6	45	from	effects	1131:1137	arg1	profiles					1163:1170	the chemical profiles	1150:1170	the chemical profiles of nine common short-chain fatty acids (SCFAs) produced by intestinal flora metabolism in CIALI mice	1150:1271	GCMS was further used to determine the effects of AERP on the chemical profiles of nine common short-chain fatty acids (SCFAs) produced by intestinal flora metabolism in CIALI mice.
30600007	3	46	theme	21.2	628:631	arg1	ratio					591:595	a molar ratio	583:595	a molar ratio of 22.2: 10.3: 8.1: 32.7: 5.7: 21.2	583:631	AERP was composed of Ara, Rha, GlcA, Man, Glc, and Gal in a molar ratio of 22.2: 10.3: 8.1: 32.7: 5.7: 21.2 along with a small number of sulfate (3.38%) and acetyl (4.87%) groups.
30600007	6	47	theme	AERP	1142:1145	arg1	effects					1131:1137	the effects	1127:1137	the effects of AERP on the chemical profiles of nine common short-chain fatty acids (SCFAs) produced by intestinal flora metabolism in CIALI mice	1127:1271	GCMS was further used to determine the effects of AERP on the chemical profiles of nine common short-chain fatty acids (SCFAs) produced by intestinal flora metabolism in CIALI mice.
30600007	5	48	theme	biochemical	1049:1059	arg1	examinations					1061:1072	biochemical examinations	1049:1072	biochemical examinations	1049:1072	Studies to evaluate hepatoprotective potentials of AERP showed that they had potent hepatoprotective effects in vivo in carbon tetrachloride-induced acute liver injury (CIALI) in mice by histopathological evaluation, biochemical examinations and ELISA assays.
30600007	4	49	theme	Man	741:743	arg1	linkages					729:736	The abundant glycosidic linkages	705:736	The abundant glycosidic linkages of Man, Ara, Gal, and Rha	705:762	The abundant glycosidic linkages of Man, Ara, Gal, and Rha were observed as more than 90% of all the monosaccharides detected.
30600007	4	49	theme	Man	741:743	arg1	%					793:793	more than 90%	781:793	more than 90% of all the monosaccharides detected	781:829	The abundant glycosidic linkages of Man, Ara, Gal, and Rha were observed as more than 90% of all the monosaccharides detected.
30600007	4	49	theme	Man	741:743	arg1	monosaccharides					806:820	all the monosaccharides	798:820	all the monosaccharides detected	798:829	The abundant glycosidic linkages of Man, Ara, Gal, and Rha were observed as more than 90% of all the monosaccharides detected.
30600007	7	50	from	protection	1369:1378	arg1	mice					1394:1397	mice	1394:1397	mice	1394:1397	These findings not only provide novel insights into the pharmacological actions of AERP on the protection from CIALI in mice, but they also demonstrate that determining SCFA profiles by targeted GC-MS metabolomics is an effective technique to investigate the molecular mechanisms of the effects of plant polysaccharides on intestinal flora metabolism.
30600007	4	51	theme	Ara	746:748	arg1	linkages					729:736	The abundant glycosidic linkages	705:736	The abundant glycosidic linkages of Man, Ara, Gal, and Rha	705:762	The abundant glycosidic linkages of Man, Ara, Gal, and Rha were observed as more than 90% of all the monosaccharides detected.
30600007	4	51	theme	Ara	746:748	arg1	%					793:793	more than 90%	781:793	more than 90% of all the monosaccharides detected	781:829	The abundant glycosidic linkages of Man, Ara, Gal, and Rha were observed as more than 90% of all the monosaccharides detected.
30600007	4	51	theme	Ara	746:748	arg1	monosaccharides					806:820	all the monosaccharides	798:820	all the monosaccharides detected	798:829	The abundant glycosidic linkages of Man, Ara, Gal, and Rha were observed as more than 90% of all the monosaccharides detected.
30600007	3	52	theme	small	646:650	arg1	number					652:657	a small number	644:657	a small number of sulfate (3.38%) and acetyl (4.87%) groups	644:702	AERP was composed of Ara, Rha, GlcA, Man, Glc, and Gal in a molar ratio of 22.2: 10.3: 8.1: 32.7: 5.7: 21.2 along with a small number of sulfate (3.38%) and acetyl (4.87%) groups.
30600007	7	53	from	CIALI	1385:1389	arg1	protection					1369:1378	the protection	1365:1378	the protection from CIALI in mice	1365:1397	These findings not only provide novel insights into the pharmacological actions of AERP on the protection from CIALI in mice, but they also demonstrate that determining SCFA profiles by targeted GC-MS metabolomics is an effective technique to investigate the molecular mechanisms of the effects of plant polysaccharides on intestinal flora metabolism.
30600007	4	54	theme	abundant	709:716	arg1	linkages					729:736	The abundant glycosidic linkages	705:736	The abundant glycosidic linkages of Man, Ara, Gal, and Rha	705:762	The abundant glycosidic linkages of Man, Ara, Gal, and Rha were observed as more than 90% of all the monosaccharides detected.
30600007	4	54	theme	abundant	709:716	arg1	%					793:793	more than 90%	781:793	more than 90% of all the monosaccharides detected	781:829	The abundant glycosidic linkages of Man, Ara, Gal, and Rha were observed as more than 90% of all the monosaccharides detected.
30600007	4	54	theme	abundant	709:716	arg1	monosaccharides					806:820	all the monosaccharides	798:820	all the monosaccharides detected	798:829	The abundant glycosidic linkages of Man, Ara, Gal, and Rha were observed as more than 90% of all the monosaccharides detected.
30600007	5	55	from	injury	993:998	arg1	mice					1011:1014	mice	1011:1014	mice	1011:1014	Studies to evaluate hepatoprotective potentials of AERP showed that they had potent hepatoprotective effects in vivo in carbon tetrachloride-induced acute liver injury (CIALI) in mice by histopathological evaluation, biochemical examinations and ELISA assays.
30600007	7	56	theme	flora	1608:1612	arg1	metabolism					1614:1623	intestinal flora metabolism	1597:1623	intestinal flora metabolism	1597:1623	These findings not only provide novel insights into the pharmacological actions of AERP on the protection from CIALI in mice, but they also demonstrate that determining SCFA profiles by targeted GC-MS metabolomics is an effective technique to investigate the molecular mechanisms of the effects of plant polysaccharides on intestinal flora metabolism.
30600007	0	57	theme	Aralia	61:66	arg1	polysaccharides					84:98	Aralia elata root bark polysaccharides	61:98	Aralia elata root bark polysaccharides	61:98	Structural characteristics and hepatoprotective potential of Aralia elata root bark polysaccharides and their effects on SCFAs produced by intestinal flora metabolism.
30600007	6	58	theme	short-chain	1187:1197	arg1	SCFAs					1212:1216	SCFAs	1212:1216	SCFAs	1212:1216	GCMS was further used to determine the effects of AERP on the chemical profiles of nine common short-chain fatty acids (SCFAs) produced by intestinal flora metabolism in CIALI mice.
30600007	6	58	theme	short-chain	1187:1197	arg1	acids					1205:1209	nine common short-chain fatty acids	1175:1209	nine common short-chain fatty acids (SCFAs) produced by intestinal flora metabolism in CIALI mice	1175:1271	GCMS was further used to determine the effects of AERP on the chemical profiles of nine common short-chain fatty acids (SCFAs) produced by intestinal flora metabolism in CIALI mice.
30600007	7	59	theme	targeted	1460:1467	arg1	metabolomics					1475:1486	targeted GC-MS metabolomics	1460:1486	targeted GC-MS metabolomics	1460:1486	These findings not only provide novel insights into the pharmacological actions of AERP on the protection from CIALI in mice, but they also demonstrate that determining SCFA profiles by targeted GC-MS metabolomics is an effective technique to investigate the molecular mechanisms of the effects of plant polysaccharides on intestinal flora metabolism.
30600007	0	60	theme	flora	150:154	arg1	metabolism					156:165	intestinal flora metabolism	139:165	intestinal flora metabolism	139:165	Structural characteristics and hepatoprotective potential of Aralia elata root bark polysaccharides and their effects on SCFAs produced by intestinal flora metabolism.
30600007	5	61	theme	hepatoprotective	916:931	arg1	effects					933:939	potent hepatoprotective effects	909:939	potent hepatoprotective effects	909:939	Studies to evaluate hepatoprotective potentials of AERP showed that they had potent hepatoprotective effects in vivo in carbon tetrachloride-induced acute liver injury (CIALI) in mice by histopathological evaluation, biochemical examinations and ELISA assays.
30600007	1	62	theme	elata	234:238	arg1	AERP					252:255	AERP	252:255	AERP	252:255	The structural characteristics of the polysaccharides from Aralia elata root barks (AERP) were systematically investigated by FT-IR, HPSEC-ELSD and colorimetric methods as well as by GCMS based monosaccharide compositions, Smith degradations, and methylation analysis.
30600007	1	62	theme	elata	234:238	arg1	barks					245:249	Aralia elata root barks	227:249	Aralia elata root barks (AERP)	227:256	The structural characteristics of the polysaccharides from Aralia elata root barks (AERP) were systematically investigated by FT-IR, HPSEC-ELSD and colorimetric methods as well as by GCMS based monosaccharide compositions, Smith degradations, and methylation analysis.
30600007	5	63	dep	Studies	832:838	arg1	evaluate					843:850	evaluate	843:850	to evaluate hepatoprotective potentials of AERP	840:886	Studies to evaluate hepatoprotective potentials of AERP showed that they had potent hepatoprotective effects in vivo in carbon tetrachloride-induced acute liver injury (CIALI) in mice by histopathological evaluation, biochemical examinations and ELISA assays.
30600007	1	64	theme	methylation	415:425	arg1	analysis					427:434	methylation analysis	415:434	methylation analysis	415:434	The structural characteristics of the polysaccharides from Aralia elata root barks (AERP) were systematically investigated by FT-IR, HPSEC-ELSD and colorimetric methods as well as by GCMS based monosaccharide compositions, Smith degradations, and methylation analysis.
30600007	4	65	theme	Gal	751:753	arg1	linkages					729:736	The abundant glycosidic linkages	705:736	The abundant glycosidic linkages of Man, Ara, Gal, and Rha	705:762	The abundant glycosidic linkages of Man, Ara, Gal, and Rha were observed as more than 90% of all the monosaccharides detected.
30600007	4	65	theme	Gal	751:753	arg1	%					793:793	more than 90%	781:793	more than 90% of all the monosaccharides detected	781:829	The abundant glycosidic linkages of Man, Ara, Gal, and Rha were observed as more than 90% of all the monosaccharides detected.
30600007	4	65	theme	Gal	751:753	arg1	monosaccharides					806:820	all the monosaccharides	798:820	all the monosaccharides detected	798:829	The abundant glycosidic linkages of Man, Ara, Gal, and Rha were observed as more than 90% of all the monosaccharides detected.
30600007	1	66	from	barks	245:249	arg1	characteristics					183:197	The structural characteristics	168:197	The structural characteristics of the polysaccharides from Aralia elata root barks (AERP)	168:256	The structural characteristics of the polysaccharides from Aralia elata root barks (AERP) were systematically investigated by FT-IR, HPSEC-ELSD and colorimetric methods as well as by GCMS based monosaccharide compositions, Smith degradations, and methylation analysis.
30600007	1	66	from	barks	245:249	arg1	polysaccharides					206:220	the polysaccharides	202:220	the polysaccharides from Aralia elata root barks (AERP)	202:256	The structural characteristics of the polysaccharides from Aralia elata root barks (AERP) were systematically investigated by FT-IR, HPSEC-ELSD and colorimetric methods as well as by GCMS based monosaccharide compositions, Smith degradations, and methylation analysis.
30600007	3	67	theme	molar	585:589	arg1	ratio					591:595	a molar ratio	583:595	a molar ratio of 22.2: 10.3: 8.1: 32.7: 5.7: 21.2	583:631	AERP was composed of Ara, Rha, GlcA, Man, Glc, and Gal in a molar ratio of 22.2: 10.3: 8.1: 32.7: 5.7: 21.2 along with a small number of sulfate (3.38%) and acetyl (4.87%) groups.
30600007	7	68	theme	intestinal	1597:1606	arg1	metabolism					1614:1623	intestinal flora metabolism	1597:1623	intestinal flora metabolism	1597:1623	These findings not only provide novel insights into the pharmacological actions of AERP on the protection from CIALI in mice, but they also demonstrate that determining SCFA profiles by targeted GC-MS metabolomics is an effective technique to investigate the molecular mechanisms of the effects of plant polysaccharides on intestinal flora metabolism.
30600007	0	69	from	effects	110:116	arg1	SCFAs					121:125	SCFAs	121:125	SCFAs	121:125	Structural characteristics and hepatoprotective potential of Aralia elata root bark polysaccharides and their effects on SCFAs produced by intestinal flora metabolism.
30600007	3	70	from	Man	562:564	arg1	ratio					591:595	a molar ratio	583:595	a molar ratio of 22.2: 10.3: 8.1: 32.7: 5.7: 21.2	583:631	AERP was composed of Ara, Rha, GlcA, Man, Glc, and Gal in a molar ratio of 22.2: 10.3: 8.1: 32.7: 5.7: 21.2 along with a small number of sulfate (3.38%) and acetyl (4.87%) groups.
30600007	6	71	theme	intestinal	1231:1240	arg1	metabolism					1248:1257	intestinal flora metabolism	1231:1257	intestinal flora metabolism in CIALI mice	1231:1271	GCMS was further used to determine the effects of AERP on the chemical profiles of nine common short-chain fatty acids (SCFAs) produced by intestinal flora metabolism in CIALI mice.
30600007	3	72	dep	GlcA	556:559	arg1	Rha					551:553	Ara, Rha, GlcA	546:559	Rha	551:553	AERP was composed of Ara, Rha, GlcA, Man, Glc, and Gal in a molar ratio of 22.2: 10.3: 8.1: 32.7: 5.7: 21.2 along with a small number of sulfate (3.38%) and acetyl (4.87%) groups.
30600007	7	73	theme	polysaccharides	1578:1592	arg1	effects					1561:1567	the effects	1557:1567	the effects of plant polysaccharides on intestinal flora metabolism	1557:1623	These findings not only provide novel insights into the pharmacological actions of AERP on the protection from CIALI in mice, but they also demonstrate that determining SCFA profiles by targeted GC-MS metabolomics is an effective technique to investigate the molecular mechanisms of the effects of plant polysaccharides on intestinal flora metabolism.
30600007	7	74	from	mechanisms	1543:1552	arg1	metabolism					1614:1623	intestinal flora metabolism	1597:1623	intestinal flora metabolism	1597:1623	These findings not only provide novel insights into the pharmacological actions of AERP on the protection from CIALI in mice, but they also demonstrate that determining SCFA profiles by targeted GC-MS metabolomics is an effective technique to investigate the molecular mechanisms of the effects of plant polysaccharides on intestinal flora metabolism.
30600007	5	75	theme	liver	987:991	arg1	CIALI					1001:1005	CIALI	1001:1005	CIALI	1001:1005	Studies to evaluate hepatoprotective potentials of AERP showed that they had potent hepatoprotective effects in vivo in carbon tetrachloride-induced acute liver injury (CIALI) in mice by histopathological evaluation, biochemical examinations and ELISA assays.
30600007	5	75	theme	liver	987:991	arg1	injury					993:998	carbon tetrachloride-induced acute liver injury	952:998	carbon tetrachloride-induced acute liver injury (CIALI) in mice by histopathological evaluation, biochemical examinations and ELISA assays	952:1089	Studies to evaluate hepatoprotective potentials of AERP showed that they had potent hepatoprotective effects in vivo in carbon tetrachloride-induced acute liver injury (CIALI) in mice by histopathological evaluation, biochemical examinations and ELISA assays.
30600007	4	76	theme	monosaccharides	806:820	arg1	%					793:793	more than 90%	781:793	more than 90% of all the monosaccharides detected	781:829	The abundant glycosidic linkages of Man, Ara, Gal, and Rha were observed as more than 90% of all the monosaccharides detected.
30600007	4	76	theme	monosaccharides	806:820	arg1	linkages					729:736	The abundant glycosidic linkages	705:736	The abundant glycosidic linkages of Man, Ara, Gal, and Rha	705:762	The abundant glycosidic linkages of Man, Ara, Gal, and Rha were observed as more than 90% of all the monosaccharides detected.
30600007	4	76	theme	monosaccharides	806:820	arg1	monosaccharides					806:820	all the monosaccharides	798:820	all the monosaccharides detected	798:829	The abundant glycosidic linkages of Man, Ara, Gal, and Rha were observed as more than 90% of all the monosaccharides detected.
30600007	0	77	from	characteristics	11:25	arg1	SCFAs					121:125	SCFAs	121:125	SCFAs	121:125	Structural characteristics and hepatoprotective potential of Aralia elata root bark polysaccharides and their effects on SCFAs produced by intestinal flora metabolism.
30600007	3	78	theme	groups	697:702	arg1	number					652:657	a small number	644:657	a small number of sulfate (3.38%) and acetyl (4.87%) groups	644:702	AERP was composed of Ara, Rha, GlcA, Man, Glc, and Gal in a molar ratio of 22.2: 10.3: 8.1: 32.7: 5.7: 21.2 along with a small number of sulfate (3.38%) and acetyl (4.87%) groups.
30600007	6	79	theme	acids	1205:1209	arg1	profiles					1163:1170	the chemical profiles	1150:1170	the chemical profiles of nine common short-chain fatty acids (SCFAs) produced by intestinal flora metabolism in CIALI mice	1150:1271	GCMS was further used to determine the effects of AERP on the chemical profiles of nine common short-chain fatty acids (SCFAs) produced by intestinal flora metabolism in CIALI mice.
30600007	5	80	theme	tetrachloride-induced	959:979	arg1	CIALI					1001:1005	CIALI	1001:1005	CIALI	1001:1005	Studies to evaluate hepatoprotective potentials of AERP showed that they had potent hepatoprotective effects in vivo in carbon tetrachloride-induced acute liver injury (CIALI) in mice by histopathological evaluation, biochemical examinations and ELISA assays.
30600007	5	80	theme	tetrachloride-induced	959:979	arg1	injury					993:998	carbon tetrachloride-induced acute liver injury	952:998	carbon tetrachloride-induced acute liver injury (CIALI) in mice by histopathological evaluation, biochemical examinations and ELISA assays	952:1089	Studies to evaluate hepatoprotective potentials of AERP showed that they had potent hepatoprotective effects in vivo in carbon tetrachloride-induced acute liver injury (CIALI) in mice by histopathological evaluation, biochemical examinations and ELISA assays.
30600007	2	81	theme	molecular	463:471	arg1	weights					473:479	average molecular weights	455:479	average molecular weights of AERP	455:487	The result showed average molecular weights of AERP were between 42.7 kDa and 93.9 kDa.
30600007	3	82	from	Glc	567:569	arg1	ratio					591:595	a molar ratio	583:595	a molar ratio of 22.2: 10.3: 8.1: 32.7: 5.7: 21.2	583:631	AERP was composed of Ara, Rha, GlcA, Man, Glc, and Gal in a molar ratio of 22.2: 10.3: 8.1: 32.7: 5.7: 21.2 along with a small number of sulfate (3.38%) and acetyl (4.87%) groups.
30600007	0	83	theme	hepatoprotective	31:46	arg1	potential					48:56	hepatoprotective potential	31:56	hepatoprotective potential	31:56	Structural characteristics and hepatoprotective potential of Aralia elata root bark polysaccharides and their effects on SCFAs produced by intestinal flora metabolism.
30600007	5	84	theme	histopathological	1019:1035	arg1	evaluation					1037:1046	histopathological evaluation	1019:1046	histopathological evaluation	1019:1046	Studies to evaluate hepatoprotective potentials of AERP showed that they had potent hepatoprotective effects in vivo in carbon tetrachloride-induced acute liver injury (CIALI) in mice by histopathological evaluation, biochemical examinations and ELISA assays.
30600007	6	85	theme	CIALI	1262:1266	arg1	mice					1268:1271	CIALI mice	1262:1271	CIALI mice	1262:1271	GCMS was further used to determine the effects of AERP on the chemical profiles of nine common short-chain fatty acids (SCFAs) produced by intestinal flora metabolism in CIALI mice.
30600007	5	86	theme	hepatoprotective	852:867	arg1	potentials					869:878	hepatoprotective potentials	852:878	hepatoprotective potentials of AERP	852:886	Studies to evaluate hepatoprotective potentials of AERP showed that they had potent hepatoprotective effects in vivo in carbon tetrachloride-induced acute liver injury (CIALI) in mice by histopathological evaluation, biochemical examinations and ELISA assays.
30600007	7	87	theme	effective	1494:1502	arg1	technique					1504:1512	an effective technique	1491:1512	an effective technique to investigate the molecular mechanisms of the effects of plant polysaccharides on intestinal flora metabolism	1491:1623	These findings not only provide novel insights into the pharmacological actions of AERP on the protection from CIALI in mice, but they also demonstrate that determining SCFA profiles by targeted GC-MS metabolomics is an effective technique to investigate the molecular mechanisms of the effects of plant polysaccharides on intestinal flora metabolism.
30600007	0	88	theme	elata	68:72	arg1	polysaccharides					84:98	Aralia elata root bark polysaccharides	61:98	Aralia elata root bark polysaccharides	61:98	Structural characteristics and hepatoprotective potential of Aralia elata root bark polysaccharides and their effects on SCFAs produced by intestinal flora metabolism.
30600007	7	89	from	actions	1346:1352	arg1	protection					1369:1378	the protection	1365:1378	the protection from CIALI in mice	1365:1397	These findings not only provide novel insights into the pharmacological actions of AERP on the protection from CIALI in mice, but they also demonstrate that determining SCFA profiles by targeted GC-MS metabolomics is an effective technique to investigate the molecular mechanisms of the effects of plant polysaccharides on intestinal flora metabolism.
29674255	5	0	theme	leukocytes	794:803	arg1	number					772:777	the number	768:777	the number of circulating leukocytes	768:803	The compounds also exerted generalized, anti-inflammatory effects by decreasing the number of circulating leukocytes, lowering subiliac lymph node weight and partially normalizing an altered blood cell composition.
29674255	1	1	theme	imiquimod-induced	348:364	arg1	psoriasis					366:374	imiquimod-induced psoriasis	348:374	imiquimod-induced psoriasis in mice	348:382	The therapeutic efficacy of topically applied azaphenothiazine derivatives: 9-chloro-6-acetylaminobutylquinobenzo[3,2-b][1,4]thiazine (compound 4) and 6-chloroethylureidoethyldiquino[3,2-b;2';3'-e][1,4]thiazine (compound 5) in the amelioration of inflammatory symptoms of imiquimod-induced psoriasis in mice was investigated.
29674255	1	2	from	efficacy	92:99	arg1	amelioration					307:318	the amelioration	303:318	the amelioration of inflammatory symptoms of imiquimod-induced psoriasis in mice	303:382	The therapeutic efficacy of topically applied azaphenothiazine derivatives: 9-chloro-6-acetylaminobutylquinobenzo[3,2-b][1,4]thiazine (compound 4) and 6-chloroethylureidoethyldiquino[3,2-b;2';3'-e][1,4]thiazine (compound 5) in the amelioration of inflammatory symptoms of imiquimod-induced psoriasis in mice was investigated.
29674255	6	3	theme	types	947:951	arg1	composition					922:932	the composition	918:932	the composition of main cell types in the epidermis and dermis	918:979	The changes in the composition of main cell types in the epidermis and dermis were less affected by the compounds.
29674255	1	4	theme	psoriasis	366:374	arg1	symptoms					336:343	inflammatory symptoms	323:343	inflammatory symptoms of imiquimod-induced psoriasis in mice	323:382	The therapeutic efficacy of topically applied azaphenothiazine derivatives: 9-chloro-6-acetylaminobutylquinobenzo[3,2-b][1,4]thiazine (compound 4) and 6-chloroethylureidoethyldiquino[3,2-b;2';3'-e][1,4]thiazine (compound 5) in the amelioration of inflammatory symptoms of imiquimod-induced psoriasis in mice was investigated.
29674255	1	5	from	symptoms	336:343	arg1	mice					379:382	mice	379:382	mice	379:382	The therapeutic efficacy of topically applied azaphenothiazine derivatives: 9-chloro-6-acetylaminobutylquinobenzo[3,2-b][1,4]thiazine (compound 4) and 6-chloroethylureidoethyldiquino[3,2-b;2';3'-e][1,4]thiazine (compound 5) in the amelioration of inflammatory symptoms of imiquimod-induced psoriasis in mice was investigated.
29674255	10	6	theme	pathology	1599:1607	arg1	amelioration					1574:1585	the amelioration	1570:1585	the amelioration of the skin pathology elicited in a mouse experimental model of psoriasis	1570:1659	In summary, we demonstrated the therapeutic potential of two selected azaphenotiazines in the amelioration of the skin pathology elicited in a mouse experimental model of psoriasis.
29674255	7	7	theme	human	1125:1129	arg1	culture					1148:1154	human whole blood cell culture	1125:1154	human whole blood cell culture	1125:1154	In addition, both compounds inhibited to a similar degree production of tumor necrosis factor α (TNF α) in human whole blood cell culture.
29674255	7	8	from	production	1076:1085	arg1	culture					1148:1154	human whole blood cell culture	1125:1154	human whole blood cell culture	1125:1154	In addition, both compounds inhibited to a similar degree production of tumor necrosis factor α (TNF α) in human whole blood cell culture.
29674255	6	9	theme	main	937:940	arg1	types					947:951	main cell types	937:951	main cell types in the epidermis and dermis	937:979	The changes in the composition of main cell types in the epidermis and dermis were less affected by the compounds.
29674255	8	10	theme	suppressive	1296:1306	arg1	action					1308:1313	the suppressive action	1292:1313	the suppressive action of compound 4 in this model	1292:1341	Whereas compound 5 strongly inhibited IL-8 and CXCL10 chemokines in human keratinocytes - KERTr cell line, transfected with poly(I:C), the suppressive action of compound 4 in this model was weak.
29674255	8	10	theme	suppressive	1296:1306	arg1	weak					1347:1350	weak	1347:1350	weak	1347:1350	Whereas compound 5 strongly inhibited IL-8 and CXCL10 chemokines in human keratinocytes - KERTr cell line, transfected with poly(I:C), the suppressive action of compound 4 in this model was weak.
29674255	4	11	theme	compounds	608:616	arg1	actions					593:599	The suppressive actions	577:599	The suppressive actions of the compounds	577:616	The suppressive actions of the compounds were even stronger with regard to pathological changes of the dermis.
29674255	4	11	theme	compounds	608:616	arg1	stronger					628:635	stronger	628:635	stronger	628:635	The suppressive actions of the compounds were even stronger with regard to pathological changes of the dermis.
29674255	10	12	theme	psoriasis	1651:1659	arg1	model					1642:1646	a mouse experimental model	1621:1646	a mouse experimental model of psoriasis	1621:1659	In summary, we demonstrated the therapeutic potential of two selected azaphenotiazines in the amelioration of the skin pathology elicited in a mouse experimental model of psoriasis.
29674255	9	13	theme	cell	1468:1471	arg1	lines					1473:1477	colonic cell lines	1460:1477	colonic cell lines	1460:1477	In addition, compound 5, but not compound 4, exhibited at low doses proapoptotic properties with regard to colonic cell lines.
29674255	3	14	theme	cell	564:567	arg1	layers					569:574	the cell layers	560:574	the cell layers	560:574	The application of the compounds led to thinning of the epidermis and reduction of the cell layers.
29674255	5	15	dep	generalized	715:725	arg1	anti-inflammatory					728:744	anti-inflammatory	728:744	anti-inflammatory	728:744	The compounds also exerted generalized, anti-inflammatory effects by decreasing the number of circulating leukocytes, lowering subiliac lymph node weight and partially normalizing an altered blood cell composition.
29674255	9	16	theme	proapoptotic	1421:1432	arg1	properties					1434:1443	proapoptotic properties	1421:1443	proapoptotic properties	1421:1443	In addition, compound 5, but not compound 4, exhibited at low doses proapoptotic properties with regard to colonic cell lines.
29674255	8	17	theme	cell	1253:1256	arg1	line					1258:1261	KERTr cell line	1247:1261	KERTr cell line	1247:1261	Whereas compound 5 strongly inhibited IL-8 and CXCL10 chemokines in human keratinocytes - KERTr cell line, transfected with poly(I:C), the suppressive action of compound 4 in this model was weak.
29674255	1	18	theme	therapeutic	80:90	arg1	efficacy					92:99	The therapeutic efficacy	76:99	The therapeutic efficacy of topically applied azaphenothiazine derivatives: 9-chloro-6-acetylaminobutylquinobenzo[3,2-b][1,4]thiazine (compound 4) and 6-chloroethylureidoethyldiquino[3,2-b;2';3'-e][1,4]thiazine (compound 5) in the amelioration of inflammatory symptoms of imiquimod-induced psoriasis in mice	76:382	The therapeutic efficacy of topically applied azaphenothiazine derivatives: 9-chloro-6-acetylaminobutylquinobenzo[3,2-b][1,4]thiazine (compound 4) and 6-chloroethylureidoethyldiquino[3,2-b;2';3'-e][1,4]thiazine (compound 5) in the amelioration of inflammatory symptoms of imiquimod-induced psoriasis in mice was investigated.
29674255	2	19	theme	clobetasol	425:434	arg1	Clobederm®					402:411	Clobederm®	402:411	Clobederm®	402:411	Clobederm®, containing clobetasol propioniate, served as a reference drug.
29674255	2	19	theme	clobetasol	425:434	arg1	propioniate					436:446	containing clobetasol propioniate	414:446	containing clobetasol propioniate	414:446	Clobederm®, containing clobetasol propioniate, served as a reference drug.
29674255	6	20	from	composition	922:932	arg1	dermis					974:979	dermis	974:979	dermis	974:979	The changes in the composition of main cell types in the epidermis and dermis were less affected by the compounds.
29674255	6	20	from	composition	922:932	arg1	epidermis					960:968	epidermis	960:968	epidermis	960:968	The changes in the composition of main cell types in the epidermis and dermis were less affected by the compounds.
29674255	0	21	from	psoriasis	57:65	arg1	mice					70:73	mice	70:73	mice	70:73	Topically applied azaphenothiazines inhibit experimental psoriasis in mice.
29674255	7	22	theme	blood	1137:1141	arg1	culture					1148:1154	human whole blood cell culture	1125:1154	human whole blood cell culture	1125:1154	In addition, both compounds inhibited to a similar degree production of tumor necrosis factor α (TNF α) in human whole blood cell culture.
29674255	7	23	theme	tumor	1090:1094	arg1	factor					1105:1110	tumor necrosis factor α	1090:1112	tumor necrosis factor α (TNF α)	1090:1120	In addition, both compounds inhibited to a similar degree production of tumor necrosis factor α (TNF α) in human whole blood cell culture.
29674255	7	23	theme	tumor	1090:1094	arg1	TNF					1115:1117	TNF α	1115:1119	TNF α	1115:1119	In addition, both compounds inhibited to a similar degree production of tumor necrosis factor α (TNF α) in human whole blood cell culture.
29674255	10	24	theme	therapeutic	1512:1522	arg1	potential					1524:1532	the therapeutic potential	1508:1532	the therapeutic potential of two selected azaphenotiazines in the amelioration of the skin pathology elicited in a mouse experimental model of psoriasis	1508:1659	In summary, we demonstrated the therapeutic potential of two selected azaphenotiazines in the amelioration of the skin pathology elicited in a mouse experimental model of psoriasis.
29674255	2	25	theme	containing	414:423	arg1	Clobederm®					402:411	Clobederm®	402:411	Clobederm®	402:411	Clobederm®, containing clobetasol propioniate, served as a reference drug.
29674255	2	25	theme	containing	414:423	arg1	propioniate					436:446	containing clobetasol propioniate	414:446	containing clobetasol propioniate	414:446	Clobederm®, containing clobetasol propioniate, served as a reference drug.
29674255	0	26	theme	applied	10:16	arg1	azaphenothiazines					18:34	Topically applied azaphenothiazines	0:34	Topically applied azaphenothiazines	0:34	Topically applied azaphenothiazines inhibit experimental psoriasis in mice.
29674255	6	27	from	types	947:951	arg1	dermis					974:979	dermis	974:979	dermis	974:979	The changes in the composition of main cell types in the epidermis and dermis were less affected by the compounds.
29674255	6	27	from	types	947:951	arg1	epidermis					960:968	epidermis	960:968	epidermis	960:968	The changes in the composition of main cell types in the epidermis and dermis were less affected by the compounds.
29674255	7	28	theme	necrosis	1096:1103	arg1	factor					1105:1110	tumor necrosis factor α	1090:1112	tumor necrosis factor α (TNF α)	1090:1120	In addition, both compounds inhibited to a similar degree production of tumor necrosis factor α (TNF α) in human whole blood cell culture.
29674255	7	28	theme	necrosis	1096:1103	arg1	TNF					1115:1117	TNF α	1115:1119	TNF α	1115:1119	In addition, both compounds inhibited to a similar degree production of tumor necrosis factor α (TNF α) in human whole blood cell culture.
29674255	1	29	dep	thiazine	201:208	arg1	compound					211:218	compound 4	211:220	compound 4	211:220	The therapeutic efficacy of topically applied azaphenothiazine derivatives: 9-chloro-6-acetylaminobutylquinobenzo[3,2-b][1,4]thiazine (compound 4) and 6-chloroethylureidoethyldiquino[3,2-b;2';3'-e][1,4]thiazine (compound 5) in the amelioration of inflammatory symptoms of imiquimod-induced psoriasis in mice was investigated.
29674255	8	30	theme	KERTr	1247:1251	arg1	line					1258:1261	KERTr cell line	1247:1261	KERTr cell line	1247:1261	Whereas compound 5 strongly inhibited IL-8 and CXCL10 chemokines in human keratinocytes - KERTr cell line, transfected with poly(I:C), the suppressive action of compound 4 in this model was weak.
29674255	6	31	theme	cell	942:945	arg1	types					947:951	main cell types	937:951	main cell types in the epidermis and dermis	937:979	The changes in the composition of main cell types in the epidermis and dermis were less affected by the compounds.
29674255	7	32	theme	cell	1143:1146	arg1	culture					1148:1154	human whole blood cell culture	1125:1154	human whole blood cell culture	1125:1154	In addition, both compounds inhibited to a similar degree production of tumor necrosis factor α (TNF α) in human whole blood cell culture.
29674255	7	33	theme	factor	1105:1110	arg1	production					1076:1085	production	1076:1085	production of tumor necrosis factor α (TNF α) in human whole blood cell culture	1076:1154	In addition, both compounds inhibited to a similar degree production of tumor necrosis factor α (TNF α) in human whole blood cell culture.
29674255	8	34	theme	compound	1318:1325	arg1	action					1308:1313	the suppressive action	1292:1313	the suppressive action of compound 4 in this model	1292:1341	Whereas compound 5 strongly inhibited IL-8 and CXCL10 chemokines in human keratinocytes - KERTr cell line, transfected with poly(I:C), the suppressive action of compound 4 in this model was weak.
29674255	8	34	theme	compound	1318:1325	arg1	weak					1347:1350	weak	1347:1350	weak	1347:1350	Whereas compound 5 strongly inhibited IL-8 and CXCL10 chemokines in human keratinocytes - KERTr cell line, transfected with poly(I:C), the suppressive action of compound 4 in this model was weak.
29674255	4	35	theme	dermis	680:685	arg1	changes					665:671	pathological changes	652:671	pathological changes of the dermis	652:685	The suppressive actions of the compounds were even stronger with regard to pathological changes of the dermis.
29674255	2	36	theme	reference	461:469	arg1	drug					471:474	a reference drug	459:474	a reference drug	459:474	Clobederm®, containing clobetasol propioniate, served as a reference drug.
29674255	2	36	theme	reference	461:469	arg1	Clobederm®					402:411	Clobederm®	402:411	Clobederm®	402:411	Clobederm®, containing clobetasol propioniate, served as a reference drug.
29674255	5	37	theme	generalized	715:725	arg1	effects					746:752	generalized, anti-inflammatory effects	715:752	generalized, anti-inflammatory effects	715:752	The compounds also exerted generalized, anti-inflammatory effects by decreasing the number of circulating leukocytes, lowering subiliac lymph node weight and partially normalizing an altered blood cell composition.
29674255	8	38	theme	IL-8	1195:1198	arg1	chemokines					1211:1220	IL-8 and CXCL10 chemokines	1195:1220	IL-8 and CXCL10 chemokines	1195:1220	Whereas compound 5 strongly inhibited IL-8 and CXCL10 chemokines in human keratinocytes - KERTr cell line, transfected with poly(I:C), the suppressive action of compound 4 in this model was weak.
29674255	1	39	theme	applied	114:120	arg1	azaphenothiazine					122:137	topically applied azaphenothiazine	104:137	topically applied azaphenothiazine derivatives: 9-chloro-6-acetylaminobutylquinobenzo[3,2-b][1,4]thiazine (compound 4) and 6-chloroethylureidoethyldiquino[3,2-b;2';3'-e][1,4]thiazine (compound 5)	104:298	The therapeutic efficacy of topically applied azaphenothiazine derivatives: 9-chloro-6-acetylaminobutylquinobenzo[3,2-b][1,4]thiazine (compound 4) and 6-chloroethylureidoethyldiquino[3,2-b;2';3'-e][1,4]thiazine (compound 5) in the amelioration of inflammatory symptoms of imiquimod-induced psoriasis in mice was investigated.
29674255	4	40	theme	suppressive	581:591	arg1	actions					593:599	The suppressive actions	577:599	The suppressive actions of the compounds	577:616	The suppressive actions of the compounds were even stronger with regard to pathological changes of the dermis.
29674255	4	40	theme	suppressive	581:591	arg1	stronger					628:635	stronger	628:635	stronger	628:635	The suppressive actions of the compounds were even stronger with regard to pathological changes of the dermis.
29674255	3	41	theme	reduction	547:555	arg1	thinning					517:524	thinning	517:524	thinning of the epidermis and reduction of the cell layers	517:574	The application of the compounds led to thinning of the epidermis and reduction of the cell layers.
29674255	6	42	dep	epidermis	960:968	arg1	the					956:958	the	956:958	the	956:958	The changes in the composition of main cell types in the epidermis and dermis were less affected by the compounds.
29674255	1	43	theme	azaphenothiazine	122:137	arg1	efficacy					92:99	The therapeutic efficacy	76:99	The therapeutic efficacy of topically applied azaphenothiazine derivatives: 9-chloro-6-acetylaminobutylquinobenzo[3,2-b][1,4]thiazine (compound 4) and 6-chloroethylureidoethyldiquino[3,2-b;2';3'-e][1,4]thiazine (compound 5) in the amelioration of inflammatory symptoms of imiquimod-induced psoriasis in mice	76:382	The therapeutic efficacy of topically applied azaphenothiazine derivatives: 9-chloro-6-acetylaminobutylquinobenzo[3,2-b][1,4]thiazine (compound 4) and 6-chloroethylureidoethyldiquino[3,2-b;2';3'-e][1,4]thiazine (compound 5) in the amelioration of inflammatory symptoms of imiquimod-induced psoriasis in mice was investigated.
29674255	5	44	theme	altered	871:877	arg1	composition					890:900	an altered blood cell composition	868:900	an altered blood cell composition	868:900	The compounds also exerted generalized, anti-inflammatory effects by decreasing the number of circulating leukocytes, lowering subiliac lymph node weight and partially normalizing an altered blood cell composition.
29674255	1	45	from	psoriasis	366:374	arg1	mice					379:382	mice	379:382	mice	379:382	The therapeutic efficacy of topically applied azaphenothiazine derivatives: 9-chloro-6-acetylaminobutylquinobenzo[3,2-b][1,4]thiazine (compound 4) and 6-chloroethylureidoethyldiquino[3,2-b;2';3'-e][1,4]thiazine (compound 5) in the amelioration of inflammatory symptoms of imiquimod-induced psoriasis in mice was investigated.
29674255	9	46	theme	low	1411:1413	arg1	doses					1415:1419	low doses	1411:1419	low doses	1411:1419	In addition, compound 5, but not compound 4, exhibited at low doses proapoptotic properties with regard to colonic cell lines.
29674255	10	47	theme	skin	1594:1597	arg1	pathology					1599:1607	the skin pathology	1590:1607	the skin pathology elicited in a mouse experimental model of psoriasis	1590:1659	In summary, we demonstrated the therapeutic potential of two selected azaphenotiazines in the amelioration of the skin pathology elicited in a mouse experimental model of psoriasis.
29674255	5	48	theme	blood	879:883	arg1	composition					890:900	an altered blood cell composition	868:900	an altered blood cell composition	868:900	The compounds also exerted generalized, anti-inflammatory effects by decreasing the number of circulating leukocytes, lowering subiliac lymph node weight and partially normalizing an altered blood cell composition.
29674255	0	49	theme	experimental	44:55	arg1	psoriasis					57:65	experimental psoriasis	44:65	experimental psoriasis in mice	44:73	Topically applied azaphenothiazines inhibit experimental psoriasis in mice.
29674255	5	50	theme	subiliac	815:822	arg1	node					830:833	subiliac lymph node	815:833	subiliac lymph node weight	815:840	The compounds also exerted generalized, anti-inflammatory effects by decreasing the number of circulating leukocytes, lowering subiliac lymph node weight and partially normalizing an altered blood cell composition.
29674255	1	51	from	mice	379:382	arg1	symptoms					336:343	inflammatory symptoms	323:343	inflammatory symptoms of imiquimod-induced psoriasis in mice	323:382	The therapeutic efficacy of topically applied azaphenothiazine derivatives: 9-chloro-6-acetylaminobutylquinobenzo[3,2-b][1,4]thiazine (compound 4) and 6-chloroethylureidoethyldiquino[3,2-b;2';3'-e][1,4]thiazine (compound 5) in the amelioration of inflammatory symptoms of imiquimod-induced psoriasis in mice was investigated.
29674255	4	52	theme	pathological	652:663	arg1	changes					665:671	pathological changes	652:671	pathological changes of the dermis	652:685	The suppressive actions of the compounds were even stronger with regard to pathological changes of the dermis.
29674255	5	53	theme	lymph	824:828	arg1	node					830:833	subiliac lymph node	815:833	subiliac lymph node weight	815:840	The compounds also exerted generalized, anti-inflammatory effects by decreasing the number of circulating leukocytes, lowering subiliac lymph node weight and partially normalizing an altered blood cell composition.
29674255	6	54	from	changes	907:913	arg1	composition					922:932	the composition	918:932	the composition of main cell types in the epidermis and dermis	918:979	The changes in the composition of main cell types in the epidermis and dermis were less affected by the compounds.
29674255	6	55	from	epidermis	960:968	arg1	composition					922:932	the composition	918:932	the composition of main cell types in the epidermis and dermis	918:979	The changes in the composition of main cell types in the epidermis and dermis were less affected by the compounds.
29674255	10	56	theme	experimental	1629:1640	arg1	model					1642:1646	a mouse experimental model	1621:1646	a mouse experimental model of psoriasis	1621:1659	In summary, we demonstrated the therapeutic potential of two selected azaphenotiazines in the amelioration of the skin pathology elicited in a mouse experimental model of psoriasis.
29674255	5	57	theme	node	830:833	arg1	weight					835:840	subiliac lymph node weight	815:840	subiliac lymph node weight	815:840	The compounds also exerted generalized, anti-inflammatory effects by decreasing the number of circulating leukocytes, lowering subiliac lymph node weight and partially normalizing an altered blood cell composition.
29674255	1	58	theme	inflammatory	323:334	arg1	symptoms					336:343	inflammatory symptoms	323:343	inflammatory symptoms of imiquimod-induced psoriasis in mice	323:382	The therapeutic efficacy of topically applied azaphenothiazine derivatives: 9-chloro-6-acetylaminobutylquinobenzo[3,2-b][1,4]thiazine (compound 4) and 6-chloroethylureidoethyldiquino[3,2-b;2';3'-e][1,4]thiazine (compound 5) in the amelioration of inflammatory symptoms of imiquimod-induced psoriasis in mice was investigated.
29674255	7	59	theme	similar	1061:1067	arg1	degree					1069:1074	a similar degree	1059:1074	a similar degree	1059:1074	In addition, both compounds inhibited to a similar degree production of tumor necrosis factor α (TNF α) in human whole blood cell culture.
29674255	10	60	theme	mouse	1623:1627	arg1	model					1642:1646	a mouse experimental model	1621:1646	a mouse experimental model of psoriasis	1621:1659	In summary, we demonstrated the therapeutic potential of two selected azaphenotiazines in the amelioration of the skin pathology elicited in a mouse experimental model of psoriasis.
29674255	8	61	theme	CXCL10	1204:1209	arg1	chemokines					1211:1220	IL-8 and CXCL10 chemokines	1195:1220	IL-8 and CXCL10 chemokines	1195:1220	Whereas compound 5 strongly inhibited IL-8 and CXCL10 chemokines in human keratinocytes - KERTr cell line, transfected with poly(I:C), the suppressive action of compound 4 in this model was weak.
29674255	10	62	theme	azaphenotiazines	1550:1565	arg1	potential					1524:1532	the therapeutic potential	1508:1532	the therapeutic potential of two selected azaphenotiazines in the amelioration of the skin pathology elicited in a mouse experimental model of psoriasis	1508:1659	In summary, we demonstrated the therapeutic potential of two selected azaphenotiazines in the amelioration of the skin pathology elicited in a mouse experimental model of psoriasis.
29674255	1	63	theme	symptoms	336:343	arg1	amelioration					307:318	the amelioration	303:318	the amelioration of inflammatory symptoms of imiquimod-induced psoriasis in mice	303:382	The therapeutic efficacy of topically applied azaphenothiazine derivatives: 9-chloro-6-acetylaminobutylquinobenzo[3,2-b][1,4]thiazine (compound 4) and 6-chloroethylureidoethyldiquino[3,2-b;2';3'-e][1,4]thiazine (compound 5) in the amelioration of inflammatory symptoms of imiquimod-induced psoriasis in mice was investigated.
29674255	6	64	from	dermis	974:979	arg1	composition					922:932	the composition	918:932	the composition of main cell types in the epidermis and dermis	918:979	The changes in the composition of main cell types in the epidermis and dermis were less affected by the compounds.
29674255	7	65	theme	whole	1131:1135	arg1	culture					1148:1154	human whole blood cell culture	1125:1154	human whole blood cell culture	1125:1154	In addition, both compounds inhibited to a similar degree production of tumor necrosis factor α (TNF α) in human whole blood cell culture.
29674255	3	66	theme	compounds	500:508	arg1	application					481:491	The application	477:491	The application of the compounds	477:508	The application of the compounds led to thinning of the epidermis and reduction of the cell layers.
29674255	3	67	theme	layers	569:574	arg1	reduction					547:555	reduction	547:555	reduction	547:555	The application of the compounds led to thinning of the epidermis and reduction of the cell layers.
29674255	3	67	theme	layers	569:574	arg1	epidermis					533:541	epidermis	533:541	epidermis	533:541	The application of the compounds led to thinning of the epidermis and reduction of the cell layers.
29674255	8	68	from	action	1308:1313	arg1	model					1337:1341	this model	1332:1341	this model	1332:1341	Whereas compound 5 strongly inhibited IL-8 and CXCL10 chemokines in human keratinocytes - KERTr cell line, transfected with poly(I:C), the suppressive action of compound 4 in this model was weak.
29674255	9	69	theme	colonic	1460:1466	arg1	lines					1473:1477	colonic cell lines	1460:1477	colonic cell lines	1460:1477	In addition, compound 5, but not compound 4, exhibited at low doses proapoptotic properties with regard to colonic cell lines.
29674255	10	70	from	potential	1524:1532	arg1	amelioration					1574:1585	the amelioration	1570:1585	the amelioration of the skin pathology elicited in a mouse experimental model of psoriasis	1570:1659	In summary, we demonstrated the therapeutic potential of two selected azaphenotiazines in the amelioration of the skin pathology elicited in a mouse experimental model of psoriasis.
29674255	8	71	dep	keratinocytes	1231:1243	arg1	line					1258:1261	KERTr cell line	1247:1261	KERTr cell line	1247:1261	Whereas compound 5 strongly inhibited IL-8 and CXCL10 chemokines in human keratinocytes - KERTr cell line, transfected with poly(I:C), the suppressive action of compound 4 in this model was weak.
29674255	8	72	theme	I	1286:1286	arg1	C					1288:1288	I:C	1286:1288	I:C	1286:1288	Whereas compound 5 strongly inhibited IL-8 and CXCL10 chemokines in human keratinocytes - KERTr cell line, transfected with poly(I:C), the suppressive action of compound 4 in this model was weak.
29674255	8	72	theme	I	1286:1286	arg1	poly					1281:1284	poly	1281:1284	poly(I:C)	1281:1289	Whereas compound 5 strongly inhibited IL-8 and CXCL10 chemokines in human keratinocytes - KERTr cell line, transfected with poly(I:C), the suppressive action of compound 4 in this model was weak.
29674255	5	73	theme	cell	885:888	arg1	composition					890:900	an altered blood cell composition	868:900	an altered blood cell composition	868:900	The compounds also exerted generalized, anti-inflammatory effects by decreasing the number of circulating leukocytes, lowering subiliac lymph node weight and partially normalizing an altered blood cell composition.
29674255	3	74	theme	epidermis	533:541	arg1	thinning					517:524	thinning	517:524	thinning of the epidermis and reduction of the cell layers	517:574	The application of the compounds led to thinning of the epidermis and reduction of the cell layers.
29674255	8	75	theme	human	1225:1229	arg1	keratinocytes					1231:1243	human keratinocytes	1225:1243	human keratinocytes - KERTr cell line, transfected with poly(I:C)	1225:1289	Whereas compound 5 strongly inhibited IL-8 and CXCL10 chemokines in human keratinocytes - KERTr cell line, transfected with poly(I:C), the suppressive action of compound 4 in this model was weak.
29674255	5	76	theme	circulating	782:792	arg1	leukocytes					794:803	circulating leukocytes	782:803	circulating leukocytes	782:803	The compounds also exerted generalized, anti-inflammatory effects by decreasing the number of circulating leukocytes, lowering subiliac lymph node weight and partially normalizing an altered blood cell composition.
29674255	1	77	dep	azaphenothiazine	122:137	arg1	thiazine					201:208	thiazine	201:208	thiazine	201:208	The therapeutic efficacy of topically applied azaphenothiazine derivatives: 9-chloro-6-acetylaminobutylquinobenzo[3,2-b][1,4]thiazine (compound 4) and 6-chloroethylureidoethyldiquino[3,2-b;2';3'-e][1,4]thiazine (compound 5) in the amelioration of inflammatory symptoms of imiquimod-induced psoriasis in mice was investigated.
29674255	1	77	dep	azaphenothiazine	122:137	arg1	6-chloroethylureidoethyldiquino[3,2-b					227:263	6-chloroethylureidoethyldiquino[3,2-b	227:263	6-chloroethylureidoethyldiquino[3,2-b	227:263	The therapeutic efficacy of topically applied azaphenothiazine derivatives: 9-chloro-6-acetylaminobutylquinobenzo[3,2-b][1,4]thiazine (compound 4) and 6-chloroethylureidoethyldiquino[3,2-b;2';3'-e][1,4]thiazine (compound 5) in the amelioration of inflammatory symptoms of imiquimod-induced psoriasis in mice was investigated.
29674255	1	77	dep	azaphenothiazine	122:137	arg1	derivatives					139:149	derivatives	139:149	topically applied azaphenothiazine derivatives: 9-chloro-6-acetylaminobutylquinobenzo[3,2-b][1,4]thiazine (compound 4) and 6-chloroethylureidoethyldiquino[3,2-b;2';3'-e][1,4]thiazine (compound 5)	104:298	The therapeutic efficacy of topically applied azaphenothiazine derivatives: 9-chloro-6-acetylaminobutylquinobenzo[3,2-b][1,4]thiazine (compound 4) and 6-chloroethylureidoethyldiquino[3,2-b;2';3'-e][1,4]thiazine (compound 5) in the amelioration of inflammatory symptoms of imiquimod-induced psoriasis in mice was investigated.
29674255	1	77	dep	azaphenothiazine	122:137	arg1	2					265:265	2	265:265	2	265:265	The therapeutic efficacy of topically applied azaphenothiazine derivatives: 9-chloro-6-acetylaminobutylquinobenzo[3,2-b][1,4]thiazine (compound 4) and 6-chloroethylureidoethyldiquino[3,2-b;2';3'-e][1,4]thiazine (compound 5) in the amelioration of inflammatory symptoms of imiquimod-induced psoriasis in mice was investigated.
29674255	1	77	dep	azaphenothiazine	122:137	arg1	thiazine					278:285	3'-e][1,4]thiazine	268:285	topically applied azaphenothiazine derivatives: 9-chloro-6-acetylaminobutylquinobenzo[3,2-b][1,4]thiazine (compound 4) and 6-chloroethylureidoethyldiquino[3,2-b;2';3'-e][1,4]thiazine (compound 5)	104:298	The therapeutic efficacy of topically applied azaphenothiazine derivatives: 9-chloro-6-acetylaminobutylquinobenzo[3,2-b][1,4]thiazine (compound 4) and 6-chloroethylureidoethyldiquino[3,2-b;2';3'-e][1,4]thiazine (compound 5) in the amelioration of inflammatory symptoms of imiquimod-induced psoriasis in mice was investigated.
29674255	1	77	dep	azaphenothiazine	122:137	arg1	compound					288:295	compound 5	288:297	compound 5	288:297	The therapeutic efficacy of topically applied azaphenothiazine derivatives: 9-chloro-6-acetylaminobutylquinobenzo[3,2-b][1,4]thiazine (compound 4) and 6-chloroethylureidoethyldiquino[3,2-b;2';3'-e][1,4]thiazine (compound 5) in the amelioration of inflammatory symptoms of imiquimod-induced psoriasis in mice was investigated.
29674255	10	78	theme	selected	1541:1548	arg1	azaphenotiazines					1550:1565	two selected azaphenotiazines	1537:1565	two selected azaphenotiazines	1537:1565	In summary, we demonstrated the therapeutic potential of two selected azaphenotiazines in the amelioration of the skin pathology elicited in a mouse experimental model of psoriasis.
29674255	1	79	theme	[1,4	273:276	arg1	thiazine					278:285	3'-e][1,4]thiazine	268:285	topically applied azaphenothiazine derivatives: 9-chloro-6-acetylaminobutylquinobenzo[3,2-b][1,4]thiazine (compound 4) and 6-chloroethylureidoethyldiquino[3,2-b;2';3'-e][1,4]thiazine (compound 5)	104:298	The therapeutic efficacy of topically applied azaphenothiazine derivatives: 9-chloro-6-acetylaminobutylquinobenzo[3,2-b][1,4]thiazine (compound 4) and 6-chloroethylureidoethyldiquino[3,2-b;2';3'-e][1,4]thiazine (compound 5) in the amelioration of inflammatory symptoms of imiquimod-induced psoriasis in mice was investigated.
29674255	1	79	theme	[1,4	273:276	arg1	compound					288:295	compound 5	288:297	compound 5	288:297	The therapeutic efficacy of topically applied azaphenothiazine derivatives: 9-chloro-6-acetylaminobutylquinobenzo[3,2-b][1,4]thiazine (compound 4) and 6-chloroethylureidoethyldiquino[3,2-b;2';3'-e][1,4]thiazine (compound 5) in the amelioration of inflammatory symptoms of imiquimod-induced psoriasis in mice was investigated.
29851378	3	0	theme	rRNA	291:294	arg1	sequence					301:308	the 16S rRNA gene sequence	283:308	the 16S rRNA gene sequence	283:308	Analysis of the 16S rRNA gene sequence revealed that the isolate represented a member of the phylogenetic cluster of the genus Actinocorallia and was most closely related to Actinocorallia aurantiaca JCM 8201T (98.0 %) and Actinocorallia libanotica IFO 10495T (98.0 %).
29851378	7	1	theme	meso-diaminopimelic	785:803	arg1	acid					805:808	meso-diaminopimelic acid	785:808	meso-diaminopimelic acid	785:808	The peptidoglycan diamino acid of A251T was meso-diaminopimelic acid and the whole-cell hydrolysates contained glucose and ribose.
29851378	7	1	theme	meso-diaminopimelic	785:803	arg1	acid					767:770	The peptidoglycan diamino acid	741:770	The peptidoglycan diamino acid of A251T	741:779	The peptidoglycan diamino acid of A251T was meso-diaminopimelic acid and the whole-cell hydrolysates contained glucose and ribose.
29851378	10	2	theme	biochemical	1159:1169	arg1	properties					1189:1198	the biochemical and physiological properties	1155:1198	the biochemical and physiological properties	1155:1198	DNA-DNA hybridization data in combination with differences in the biochemical and physiological properties, indicated that A251T should be classified as representing a novel species within the genus Actinocorallia, for which the name Actinocorallia populi sp.
29851378	3	3	theme	gene	296:299	arg1	sequence					301:308	the 16S rRNA gene sequence	283:308	the 16S rRNA gene sequence	283:308	Analysis of the 16S rRNA gene sequence revealed that the isolate represented a member of the phylogenetic cluster of the genus Actinocorallia and was most closely related to Actinocorallia aurantiaca JCM 8201T (98.0 %) and Actinocorallia libanotica IFO 10495T (98.0 %).
29851378	3	4	theme	libanotica	509:518	arg1	10495T					524:529	Actinocorallia libanotica IFO 10495T	494:529	Actinocorallia libanotica IFO 10495T (98.0 %)	494:538	Analysis of the 16S rRNA gene sequence revealed that the isolate represented a member of the phylogenetic cluster of the genus Actinocorallia and was most closely related to Actinocorallia aurantiaca JCM 8201T (98.0 %) and Actinocorallia libanotica IFO 10495T (98.0 %).
29851378	3	4	theme	libanotica	509:518	arg1	%					537:537	98.0 %	532:537	98.0 %	532:537	Analysis of the 16S rRNA gene sequence revealed that the isolate represented a member of the phylogenetic cluster of the genus Actinocorallia and was most closely related to Actinocorallia aurantiaca JCM 8201T (98.0 %) and Actinocorallia libanotica IFO 10495T (98.0 %).
29851378	7	5	theme	A251T	775:779	arg1	acid					805:808	meso-diaminopimelic acid	785:808	meso-diaminopimelic acid	785:808	The peptidoglycan diamino acid of A251T was meso-diaminopimelic acid and the whole-cell hydrolysates contained glucose and ribose.
29851378	7	5	theme	A251T	775:779	arg1	acid					767:770	The peptidoglycan diamino acid	741:770	The peptidoglycan diamino acid of A251T	741:779	The peptidoglycan diamino acid of A251T was meso-diaminopimelic acid and the whole-cell hydrolysates contained glucose and ribose.
29851378	4	6	theme	hybridization	549:561	arg1	values					563:568	DNA-DNA hybridization values	541:568	DNA-DNA hybridization values between A251T and these strains	541:600	DNA-DNA hybridization values between A251T and these strains were 41.2 % and 45.0 %, respectively.
29851378	5	7	theme	71.5 mol	671:678	arg1	%					679:679	71.5 mol%	671:679	71.5 mol%	671:679	The G+C content of the DNA was 71.5 mol%.
29851378	5	7	theme	71.5 mol	671:678	arg1	G+C content					644:654	The G+C content	640:654	The G+C content of the DNA	640:665	The G+C content of the DNA was 71.5 mol%.
29851378	10	8	theme	populi	1342:1347	arg1	sp					1349:1350	the name Actinocorallia populi sp	1318:1350	the name Actinocorallia populi sp	1318:1350	DNA-DNA hybridization data in combination with differences in the biochemical and physiological properties, indicated that A251T should be classified as representing a novel species within the genus Actinocorallia, for which the name Actinocorallia populi sp.
29851378	3	9	theme	IFO	520:522	arg1	10495T					524:529	Actinocorallia libanotica IFO 10495T	494:529	Actinocorallia libanotica IFO 10495T (98.0 %)	494:538	Analysis of the 16S rRNA gene sequence revealed that the isolate represented a member of the phylogenetic cluster of the genus Actinocorallia and was most closely related to Actinocorallia aurantiaca JCM 8201T (98.0 %) and Actinocorallia libanotica IFO 10495T (98.0 %).
29851378	3	9	theme	IFO	520:522	arg1	%					537:537	98.0 %	532:537	98.0 %	532:537	Analysis of the 16S rRNA gene sequence revealed that the isolate represented a member of the phylogenetic cluster of the genus Actinocorallia and was most closely related to Actinocorallia aurantiaca JCM 8201T (98.0 %) and Actinocorallia libanotica IFO 10495T (98.0 %).
29851378	7	10	theme	diamino	759:765	arg1	acid					805:808	meso-diaminopimelic acid	785:808	meso-diaminopimelic acid	785:808	The peptidoglycan diamino acid of A251T was meso-diaminopimelic acid and the whole-cell hydrolysates contained glucose and ribose.
29851378	7	10	theme	diamino	759:765	arg1	acid					767:770	The peptidoglycan diamino acid	741:770	The peptidoglycan diamino acid of A251T	741:779	The peptidoglycan diamino acid of A251T was meso-diaminopimelic acid and the whole-cell hydrolysates contained glucose and ribose.
29851378	4	11	theme	DNA-DNA	541:547	arg1	values					563:568	DNA-DNA hybridization values	541:568	DNA-DNA hybridization values between A251T and these strains	541:600	DNA-DNA hybridization values between A251T and these strains were 41.2 % and 45.0 %, respectively.
29851378	9	12	theme	phospholipid	927:938	arg1	profile					940:946	The phospholipid profile	923:946	The phospholipid profile	923:946	The phospholipid profile included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, an undefined aminophospholipid and two undefined phospholipids.
29851378	2	13	theme	polyphasic	250:259	arg1	taxonomy					261:268	polyphasic taxonomy	250:268	polyphasic taxonomy	250:268	An endophytic actinobacterium, strain A251T, was isolated from the root of Populus adenopoda Maxim and subjected to characterization using polyphasic taxonomy.
29851378	3	14	theme	genus	392:396	arg1	Actinocorallia					398:411	the genus Actinocorallia	388:411	the genus Actinocorallia	388:411	Analysis of the 16S rRNA gene sequence revealed that the isolate represented a member of the phylogenetic cluster of the genus Actinocorallia and was most closely related to Actinocorallia aurantiaca JCM 8201T (98.0 %) and Actinocorallia libanotica IFO 10495T (98.0 %).
29851378	2	15	theme	endophytic	114:123	arg1	actinobacterium					125:139	An endophytic actinobacterium	111:139	An endophytic actinobacterium	111:139	An endophytic actinobacterium, strain A251T, was isolated from the root of Populus adenopoda Maxim and subjected to characterization using polyphasic taxonomy.
29851378	2	15	theme	endophytic	114:123	arg1	A251T					149:153	strain A251T	142:153	strain A251T	142:153	An endophytic actinobacterium, strain A251T, was isolated from the root of Populus adenopoda Maxim and subjected to characterization using polyphasic taxonomy.
29851378	3	16	theme	Actinocorallia	494:507	arg1	10495T					524:529	Actinocorallia libanotica IFO 10495T	494:529	Actinocorallia libanotica IFO 10495T (98.0 %)	494:538	Analysis of the 16S rRNA gene sequence revealed that the isolate represented a member of the phylogenetic cluster of the genus Actinocorallia and was most closely related to Actinocorallia aurantiaca JCM 8201T (98.0 %) and Actinocorallia libanotica IFO 10495T (98.0 %).
29851378	3	16	theme	Actinocorallia	494:507	arg1	%					537:537	98.0 %	532:537	98.0 %	532:537	Analysis of the 16S rRNA gene sequence revealed that the isolate represented a member of the phylogenetic cluster of the genus Actinocorallia and was most closely related to Actinocorallia aurantiaca JCM 8201T (98.0 %) and Actinocorallia libanotica IFO 10495T (98.0 %).
29851378	11	17	theme	=CGMCC	1383:1388	arg1	32178T					1402:1407	=CGMCC 4.7421T=JCM 32178T	1383:1407	=CGMCC 4.7421T=JCM 32178T	1383:1407	nov. is proposed, with A251T (=CGMCC 4.7421T=JCM 32178T) as the type strain.
29851378	11	17	theme	=CGMCC	1383:1388	arg1	A251T					1376:1380	A251T	1376:1380	A251T (=CGMCC 4.7421T=JCM 32178T) as the type strain	1376:1427	nov. is proposed, with A251T (=CGMCC 4.7421T=JCM 32178T) as the type strain.
29851378	7	18	contain	contained	842:850	arg1	hydrolysates					829:840	the whole-cell hydrolysates	814:840	the whole-cell hydrolysates	814:840	The peptidoglycan diamino acid of A251T was meso-diaminopimelic acid and the whole-cell hydrolysates contained glucose and ribose.
29851378	7	18	contain	contained	842:850	arg2	ribose					864:869	ribose	864:869	ribose	864:869	The peptidoglycan diamino acid of A251T was meso-diaminopimelic acid and the whole-cell hydrolysates contained glucose and ribose.
29851378	7	18	contain	contained	842:850	arg2	glucose					852:858	glucose	852:858	glucose	852:858	The peptidoglycan diamino acid of A251T was meso-diaminopimelic acid and the whole-cell hydrolysates contained glucose and ribose.
29851378	11	19	theme	4.7421T=JCM	1390:1400	arg1	32178T					1402:1407	=CGMCC 4.7421T=JCM 32178T	1383:1407	=CGMCC 4.7421T=JCM 32178T	1383:1407	nov. is proposed, with A251T (=CGMCC 4.7421T=JCM 32178T) as the type strain.
29851378	11	19	theme	4.7421T=JCM	1390:1400	arg1	A251T					1376:1380	A251T	1376:1380	A251T (=CGMCC 4.7421T=JCM 32178T) as the type strain	1376:1427	nov. is proposed, with A251T (=CGMCC 4.7421T=JCM 32178T) as the type strain.
29851378	10	20	from	differences	1140:1150	arg1	properties					1189:1198	the biochemical and physiological properties	1155:1198	the biochemical and physiological properties	1155:1198	DNA-DNA hybridization data in combination with differences in the biochemical and physiological properties, indicated that A251T should be classified as representing a novel species within the genus Actinocorallia, for which the name Actinocorallia populi sp.
29851378	7	21	theme	peptidoglycan	745:757	arg1	acid					805:808	meso-diaminopimelic acid	785:808	meso-diaminopimelic acid	785:808	The peptidoglycan diamino acid of A251T was meso-diaminopimelic acid and the whole-cell hydrolysates contained glucose and ribose.
29851378	7	21	theme	peptidoglycan	745:757	arg1	acid					767:770	The peptidoglycan diamino acid	741:770	The peptidoglycan diamino acid of A251T	741:779	The peptidoglycan diamino acid of A251T was meso-diaminopimelic acid and the whole-cell hydrolysates contained glucose and ribose.
29851378	2	22	attach	isolated	160:167	arg2	actinobacterium					125:139	An endophytic actinobacterium	111:139	An endophytic actinobacterium	111:139	An endophytic actinobacterium, strain A251T, was isolated from the root of Populus adenopoda Maxim and subjected to characterization using polyphasic taxonomy.
29851378	2	22	attach	isolated	160:167	arg2	A251T					149:153	strain A251T	142:153	strain A251T	142:153	An endophytic actinobacterium, strain A251T, was isolated from the root of Populus adenopoda Maxim and subjected to characterization using polyphasic taxonomy.
29851378	2	22	attach	isolated	160:167	arg1	root					178:181	the root	174:181	the root of Populus adenopoda Maxim	174:208	An endophytic actinobacterium, strain A251T, was isolated from the root of Populus adenopoda Maxim and subjected to characterization using polyphasic taxonomy.
29851378	0	23	theme	Actinocorallia	0:13	arg1	populi					15:20	Actinocorallia populi	0:20	Actinocorallia populi	0:20	Actinocorallia populi sp.
29851378	1	24	attach	isolated	59:66	arg2	actinomycete					46:57	an endophytic actinomycete	32:57	an endophytic actinomycete isolated from a root of Populus adenopoda (Maxim	32:106	nov., an endophytic actinomycete isolated from a root of Populus adenopoda (Maxim.)
29851378	1	24	attach	isolated	59:66	arg2	nov.					26:29	nov.	26:29	nov.	26:29	nov., an endophytic actinomycete isolated from a root of Populus adenopoda (Maxim.)
29851378	1	24	attach	isolated	59:66	arg1	root					75:78	a root	73:78	a root of Populus adenopoda (Maxim	73:106	nov., an endophytic actinomycete isolated from a root of Populus adenopoda (Maxim.)
29851378	3	25	theme	Actinocorallia	398:411	arg1	cluster					377:383	the phylogenetic cluster	360:383	the phylogenetic cluster of the genus Actinocorallia	360:411	Analysis of the 16S rRNA gene sequence revealed that the isolate represented a member of the phylogenetic cluster of the genus Actinocorallia and was most closely related to Actinocorallia aurantiaca JCM 8201T (98.0 %) and Actinocorallia libanotica IFO 10495T (98.0 %).
29851378	10	26	theme	Actinocorallia	1327:1340	arg1	sp					1349:1350	the name Actinocorallia populi sp	1318:1350	the name Actinocorallia populi sp	1318:1350	DNA-DNA hybridization data in combination with differences in the biochemical and physiological properties, indicated that A251T should be classified as representing a novel species within the genus Actinocorallia, for which the name Actinocorallia populi sp.
29851378	10	27	theme	genus	1286:1290	arg1	Actinocorallia					1292:1305	the genus Actinocorallia	1282:1305	the genus Actinocorallia	1282:1305	DNA-DNA hybridization data in combination with differences in the biochemical and physiological properties, indicated that A251T should be classified as representing a novel species within the genus Actinocorallia, for which the name Actinocorallia populi sp.
29851378	10	28	theme	novel	1261:1265	arg1	species					1267:1273	a novel species	1259:1273	a novel species	1259:1273	DNA-DNA hybridization data in combination with differences in the biochemical and physiological properties, indicated that A251T should be classified as representing a novel species within the genus Actinocorallia, for which the name Actinocorallia populi sp.
29851378	6	29	dep	C16 	705:708	arg1	 1ω9c					734:738	 1ω9c	734:738	C16 : 0, C16 : 1ω7c and C18 : 1ω9c	705:738	Major fatty acids were C16 : 0, C16 : 1ω7c and C18 : 1ω9c.
29851378	6	29	dep	C16 	705:708	arg1	C18 					729:732	C18 	729:732	C18 	729:732	Major fatty acids were C16 : 0, C16 : 1ω7c and C18 : 1ω9c.
29851378	6	29	dep	C16 	705:708	arg1	 1ω7c					719:723	 1ω7c	719:723	 1ω7c	719:723	Major fatty acids were C16 : 0, C16 : 1ω7c and C18 : 1ω9c.
29851378	6	29	dep	C16 	705:708	arg1	C16 					714:717	C16 	714:717	C16 	714:717	Major fatty acids were C16 : 0, C16 : 1ω7c and C18 : 1ω9c.
29851378	2	30	theme	Maxim	204:208	arg1	root					178:181	the root	174:181	the root of Populus adenopoda Maxim	174:208	An endophytic actinobacterium, strain A251T, was isolated from the root of Populus adenopoda Maxim and subjected to characterization using polyphasic taxonomy.
29851378	10	31	theme	hybridization	1101:1113	arg1	data					1115:1118	DNA-DNA hybridization data	1093:1118	DNA-DNA hybridization data	1093:1118	DNA-DNA hybridization data in combination with differences in the biochemical and physiological properties, indicated that A251T should be classified as representing a novel species within the genus Actinocorallia, for which the name Actinocorallia populi sp.
29851378	5	32	theme	DNA	663:665	arg1	%					679:679	71.5 mol%	671:679	71.5 mol%	671:679	The G+C content of the DNA was 71.5 mol%.
29851378	5	32	theme	DNA	663:665	arg1	G+C content					644:654	The G+C content	640:654	The G+C content of the DNA	640:665	The G+C content of the DNA was 71.5 mol%.
29851378	3	33	theme	phylogenetic	364:375	arg1	cluster					377:383	the phylogenetic cluster	360:383	the phylogenetic cluster of the genus Actinocorallia	360:411	Analysis of the 16S rRNA gene sequence revealed that the isolate represented a member of the phylogenetic cluster of the genus Actinocorallia and was most closely related to Actinocorallia aurantiaca JCM 8201T (98.0 %) and Actinocorallia libanotica IFO 10495T (98.0 %).
29851378	10	34	theme	DNA-DNA	1093:1099	arg1	data					1115:1118	DNA-DNA hybridization data	1093:1118	DNA-DNA hybridization data	1093:1118	DNA-DNA hybridization data in combination with differences in the biochemical and physiological properties, indicated that A251T should be classified as representing a novel species within the genus Actinocorallia, for which the name Actinocorallia populi sp.
29851378	2	35	theme	strain	142:147	arg1	actinobacterium					125:139	An endophytic actinobacterium	111:139	An endophytic actinobacterium	111:139	An endophytic actinobacterium, strain A251T, was isolated from the root of Populus adenopoda Maxim and subjected to characterization using polyphasic taxonomy.
29851378	2	35	theme	strain	142:147	arg1	A251T					149:153	strain A251T	142:153	strain A251T	142:153	An endophytic actinobacterium, strain A251T, was isolated from the root of Populus adenopoda Maxim and subjected to characterization using polyphasic taxonomy.
29851378	3	36	theme	JCM	471:473	arg1	8201T					475:479	Actinocorallia aurantiaca JCM 8201T	445:479	Actinocorallia aurantiaca JCM 8201T (98.0 %)	445:488	Analysis of the 16S rRNA gene sequence revealed that the isolate represented a member of the phylogenetic cluster of the genus Actinocorallia and was most closely related to Actinocorallia aurantiaca JCM 8201T (98.0 %) and Actinocorallia libanotica IFO 10495T (98.0 %).
29851378	3	36	theme	JCM	471:473	arg1	%					487:487	98.0 %	482:487	98.0 %	482:487	Analysis of the 16S rRNA gene sequence revealed that the isolate represented a member of the phylogenetic cluster of the genus Actinocorallia and was most closely related to Actinocorallia aurantiaca JCM 8201T (98.0 %) and Actinocorallia libanotica IFO 10495T (98.0 %).
29851378	3	37	theme	cluster	377:383	arg1	member					350:355	a member	348:355	a member of the phylogenetic cluster of the genus Actinocorallia	348:411	Analysis of the 16S rRNA gene sequence revealed that the isolate represented a member of the phylogenetic cluster of the genus Actinocorallia and was most closely related to Actinocorallia aurantiaca JCM 8201T (98.0 %) and Actinocorallia libanotica IFO 10495T (98.0 %).
29851378	3	38	theme	16S	287:289	arg1	rRNA					291:294	the 16S rRNA	283:294	the 16S rRNA gene sequence	283:308	Analysis of the 16S rRNA gene sequence revealed that the isolate represented a member of the phylogenetic cluster of the genus Actinocorallia and was most closely related to Actinocorallia aurantiaca JCM 8201T (98.0 %) and Actinocorallia libanotica IFO 10495T (98.0 %).
29851378	6	39	theme	fatty	688:692	arg1	acids					694:698	Major fatty acids	682:698	Major fatty acids	682:698	Major fatty acids were C16 : 0, C16 : 1ω7c and C18 : 1ω9c.
29851378	1	40	theme	Maxim	102:106	arg1	root					75:78	a root	73:78	a root of Populus adenopoda (Maxim	73:106	nov., an endophytic actinomycete isolated from a root of Populus adenopoda (Maxim.)
29851378	10	41	with	combination	1123:1133	arg1	differences					1140:1150	differences	1140:1150	differences in the biochemical and physiological properties	1140:1198	DNA-DNA hybridization data in combination with differences in the biochemical and physiological properties, indicated that A251T should be classified as representing a novel species within the genus Actinocorallia, for which the name Actinocorallia populi sp.
29851378	6	42	theme	Major	682:686	arg1	acids					694:698	Major fatty acids	682:698	Major fatty acids	682:698	Major fatty acids were C16 : 0, C16 : 1ω7c and C18 : 1ω9c.
29851378	3	43	theme	Actinocorallia	445:458	arg1	8201T					475:479	Actinocorallia aurantiaca JCM 8201T	445:479	Actinocorallia aurantiaca JCM 8201T (98.0 %)	445:488	Analysis of the 16S rRNA gene sequence revealed that the isolate represented a member of the phylogenetic cluster of the genus Actinocorallia and was most closely related to Actinocorallia aurantiaca JCM 8201T (98.0 %) and Actinocorallia libanotica IFO 10495T (98.0 %).
29851378	3	43	theme	Actinocorallia	445:458	arg1	%					487:487	98.0 %	482:487	98.0 %	482:487	Analysis of the 16S rRNA gene sequence revealed that the isolate represented a member of the phylogenetic cluster of the genus Actinocorallia and was most closely related to Actinocorallia aurantiaca JCM 8201T (98.0 %) and Actinocorallia libanotica IFO 10495T (98.0 %).
29851378	7	44	theme	whole-cell	818:827	arg1	hydrolysates					829:840	the whole-cell hydrolysates	814:840	the whole-cell hydrolysates	814:840	The peptidoglycan diamino acid of A251T was meso-diaminopimelic acid and the whole-cell hydrolysates contained glucose and ribose.
29851378	9	45	theme	undefined	1068:1076	arg1	phospholipids					1078:1090	two undefined phospholipids	1064:1090	two undefined phospholipids	1064:1090	The phospholipid profile included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, an undefined aminophospholipid and two undefined phospholipids.
29851378	0	46	dep	sp	22:23	arg1	populi					15:20	Actinocorallia populi	0:20	Actinocorallia populi	0:20	Actinocorallia populi sp.
29851378	10	47	theme	name	1322:1325	arg1	sp					1349:1350	the name Actinocorallia populi sp	1318:1350	the name Actinocorallia populi sp	1318:1350	DNA-DNA hybridization data in combination with differences in the biochemical and physiological properties, indicated that A251T should be classified as representing a novel species within the genus Actinocorallia, for which the name Actinocorallia populi sp.
29851378	3	48	theme	aurantiaca	460:469	arg1	8201T					475:479	Actinocorallia aurantiaca JCM 8201T	445:479	Actinocorallia aurantiaca JCM 8201T (98.0 %)	445:488	Analysis of the 16S rRNA gene sequence revealed that the isolate represented a member of the phylogenetic cluster of the genus Actinocorallia and was most closely related to Actinocorallia aurantiaca JCM 8201T (98.0 %) and Actinocorallia libanotica IFO 10495T (98.0 %).
29851378	3	48	theme	aurantiaca	460:469	arg1	%					487:487	98.0 %	482:487	98.0 %	482:487	Analysis of the 16S rRNA gene sequence revealed that the isolate represented a member of the phylogenetic cluster of the genus Actinocorallia and was most closely related to Actinocorallia aurantiaca JCM 8201T (98.0 %) and Actinocorallia libanotica IFO 10495T (98.0 %).
29851378	10	49	theme	physiological	1175:1187	arg1	properties					1189:1198	the biochemical and physiological properties	1155:1198	the biochemical and physiological properties	1155:1198	DNA-DNA hybridization data in combination with differences in the biochemical and physiological properties, indicated that A251T should be classified as representing a novel species within the genus Actinocorallia, for which the name Actinocorallia populi sp.
29851378	3	50	theme	sequence	301:308	arg1	Analysis					271:278	Analysis	271:278	Analysis of the 16S rRNA gene sequence	271:308	Analysis of the 16S rRNA gene sequence revealed that the isolate represented a member of the phylogenetic cluster of the genus Actinocorallia and was most closely related to Actinocorallia aurantiaca JCM 8201T (98.0 %) and Actinocorallia libanotica IFO 10495T (98.0 %).
29851378	11	51	theme	type	1417:1420	arg1	strain					1422:1427	the type strain	1413:1427	the type strain	1413:1427	nov. is proposed, with A251T (=CGMCC 4.7421T=JCM 32178T) as the type strain.
29851378	1	52	theme	endophytic	35:44	arg1	actinomycete					46:57	an endophytic actinomycete	32:57	an endophytic actinomycete isolated from a root of Populus adenopoda (Maxim	32:106	nov., an endophytic actinomycete isolated from a root of Populus adenopoda (Maxim.)
29851378	1	52	theme	endophytic	35:44	arg1	nov.					26:29	nov.	26:29	nov.	26:29	nov., an endophytic actinomycete isolated from a root of Populus adenopoda (Maxim.)
29851378	8	53	theme	major	876:880	arg1	MK-9					900:903	MK-9	900:903	MK-9	900:903	The major menaquinones were MK-9(H4) and MK-9(H6).
29851378	8	53	theme	major	876:880	arg1	menaquinones					882:893	The major menaquinones	872:893	The major menaquinones	872:893	The major menaquinones were MK-9(H4) and MK-9(H6).
29722963	0	0	theme	Mouse	115:119	arg1	Brain					121:125	Mouse Brain	115:125	Mouse Brain	115:125	Detection of Cyclooxygenase-2-Derived Oxygenation Products of the Endogenous Cannabinoid 2-Arachidonoylglycerol in Mouse Brain.
29722963	3	1	link	COX-2-derived	573:585	arg1	products					604:611	COX-2-derived 2-AG oxygenation products	573:611	COX-2-derived 2-AG oxygenation products	573:611	Although this oxygenation reaction is readily observed in vitro and in intact cells, detection of COX-2-derived 2-AG oxygenation products has not been previously reported in neuronal tissue.
29722963	1	2	theme	prostaglandins	180:193	arg1	formation					167:175	the formation	163:175	the formation of prostaglandins, which are involved in immune regulation, vascular function, and synaptic signaling	163:277	Cyclooxygenase-2 (COX-2) catalyzes the formation of prostaglandins, which are involved in immune regulation, vascular function, and synaptic signaling.
29722963	6	3	theme	variety	1085:1091	arg1	PG-Gs					1093:1097	a variety PG-Gs	1083:1097	a variety PG-Gs	1083:1097	These data provide evidence that neuronal COX-2 is capable of oxygenating 2-AG to form a variety PG-Gs in vivo and support further investigation of the physiological functions of PG-Gs.
29722963	5	4	theme	PG-Gs	931:935	arg1	Formation					912:920	Formation	912:920	Formation of these PG-Gs	912:935	Formation of these PG-Gs is prevented by acute pharmacological inhibition of COX-2.
29722963	6	5	theme	further	1119:1125	arg1	investigation					1127:1139	further investigation	1119:1139	further investigation of the physiological functions of PG-Gs	1119:1179	These data provide evidence that neuronal COX-2 is capable of oxygenating 2-AG to form a variety PG-Gs in vivo and support further investigation of the physiological functions of PG-Gs.
29722963	0	6	from	Detection	0:8	arg1	Brain					121:125	Mouse Brain	115:125	Mouse Brain	115:125	Detection of Cyclooxygenase-2-Derived Oxygenation Products of the Endogenous Cannabinoid 2-Arachidonoylglycerol in Mouse Brain.
29722963	2	7	theme	cannabinoid	318:328	arg1	2-AG					360:363	2-AG	360:363	2-AG	360:363	COX-2 also inactivates the endogenous cannabinoid (eCB) 2-arachidonoylglycerol (2-AG) via oxygenation of its arachidonic acid backbone to form a variety of prostaglandin glyceryl esters (PG-Gs).
29722963	2	7	theme	cannabinoid	318:328	arg1	2-arachidonoylglycerol					336:357	the endogenous cannabinoid (eCB) 2-arachidonoylglycerol	303:357	the endogenous cannabinoid (eCB) 2-arachidonoylglycerol (2-AG)	303:364	COX-2 also inactivates the endogenous cannabinoid (eCB) 2-arachidonoylglycerol (2-AG) via oxygenation of its arachidonic acid backbone to form a variety of prostaglandin glyceryl esters (PG-Gs).
29722963	6	8	theme	PG-Gs	1175:1179	arg1	functions					1162:1170	the physiological functions	1144:1170	the physiological functions of PG-Gs	1144:1179	These data provide evidence that neuronal COX-2 is capable of oxygenating 2-AG to form a variety PG-Gs in vivo and support further investigation of the physiological functions of PG-Gs.
29722963	6	9	theme	neuronal	1029:1036	arg1	COX-2					1038:1042	neuronal COX-2	1029:1042	neuronal COX-2	1029:1042	These data provide evidence that neuronal COX-2 is capable of oxygenating 2-AG to form a variety PG-Gs in vivo and support further investigation of the physiological functions of PG-Gs.
29722963	2	10	theme	endogenous	307:316	arg1	2-AG					360:363	2-AG	360:363	2-AG	360:363	COX-2 also inactivates the endogenous cannabinoid (eCB) 2-arachidonoylglycerol (2-AG) via oxygenation of its arachidonic acid backbone to form a variety of prostaglandin glyceryl esters (PG-Gs).
29722963	2	10	theme	endogenous	307:316	arg1	2-arachidonoylglycerol					336:357	the endogenous cannabinoid (eCB) 2-arachidonoylglycerol	303:357	the endogenous cannabinoid (eCB) 2-arachidonoylglycerol (2-AG)	303:364	COX-2 also inactivates the endogenous cannabinoid (eCB) 2-arachidonoylglycerol (2-AG) via oxygenation of its arachidonic acid backbone to form a variety of prostaglandin glyceryl esters (PG-Gs).
29722963	5	11	theme	pharmacological	959:973	arg1	inhibition					975:984	acute pharmacological inhibition	953:984	acute pharmacological inhibition of COX-2	953:993	Formation of these PG-Gs is prevented by acute pharmacological inhibition of COX-2.
29722963	4	12	theme	monoacylglycerol	862:877	arg1	lipase					879:884	monoacylglycerol lipase	862:884	monoacylglycerol lipase	862:884	Here we show that 2-AG is metabolized in the brain of transgenic COX-2-overexpressing mice and mice treated with lipopolysaccharide to form multiple species of PG-Gs that are detectable only when monoacylglycerol lipase is concomitantly blocked.
29722963	5	13	theme	acute	953:957	arg1	inhibition					975:984	acute pharmacological inhibition	953:984	acute pharmacological inhibition of COX-2	953:993	Formation of these PG-Gs is prevented by acute pharmacological inhibition of COX-2.
29722963	6	14	theme	physiological	1148:1160	arg1	functions					1162:1170	the physiological functions	1144:1170	the physiological functions of PG-Gs	1144:1179	These data provide evidence that neuronal COX-2 is capable of oxygenating 2-AG to form a variety PG-Gs in vivo and support further investigation of the physiological functions of PG-Gs.
29722963	3	15	theme	intact	546:551	arg1	cells					553:557	intact cells	546:557	intact cells	546:557	Although this oxygenation reaction is readily observed in vitro and in intact cells, detection of COX-2-derived 2-AG oxygenation products has not been previously reported in neuronal tissue.
29722963	3	16	theme	COX-2-derived	573:585	arg1	products					604:611	COX-2-derived 2-AG oxygenation products	573:611	COX-2-derived 2-AG oxygenation products	573:611	Although this oxygenation reaction is readily observed in vitro and in intact cells, detection of COX-2-derived 2-AG oxygenation products has not been previously reported in neuronal tissue.
29722963	2	17	theme	backbone	406:413	arg1	oxygenation					370:380	oxygenation	370:380	oxygenation of its arachidonic acid backbone to form a variety of prostaglandin glyceryl esters (PG-Gs)	370:472	COX-2 also inactivates the endogenous cannabinoid (eCB) 2-arachidonoylglycerol (2-AG) via oxygenation of its arachidonic acid backbone to form a variety of prostaglandin glyceryl esters (PG-Gs).
29722963	3	18	theme	2-AG	587:590	arg1	products					604:611	COX-2-derived 2-AG oxygenation products	573:611	COX-2-derived 2-AG oxygenation products	573:611	Although this oxygenation reaction is readily observed in vitro and in intact cells, detection of COX-2-derived 2-AG oxygenation products has not been previously reported in neuronal tissue.
29722963	2	19	theme	acid	401:404	arg1	backbone					406:413	its arachidonic acid backbone	385:413	its arachidonic acid backbone to form a variety of prostaglandin glyceryl esters (PG-Gs)	385:472	COX-2 also inactivates the endogenous cannabinoid (eCB) 2-arachidonoylglycerol (2-AG) via oxygenation of its arachidonic acid backbone to form a variety of prostaglandin glyceryl esters (PG-Gs).
29722963	2	20	theme	esters	459:464	arg1	variety					425:431	a variety	423:431	a variety of prostaglandin glyceryl esters (PG-Gs)	423:472	COX-2 also inactivates the endogenous cannabinoid (eCB) 2-arachidonoylglycerol (2-AG) via oxygenation of its arachidonic acid backbone to form a variety of prostaglandin glyceryl esters (PG-Gs).
29722963	2	20	theme	esters	459:464	arg1	PG-Gs					467:471	PG-Gs	467:471	PG-Gs	467:471	COX-2 also inactivates the endogenous cannabinoid (eCB) 2-arachidonoylglycerol (2-AG) via oxygenation of its arachidonic acid backbone to form a variety of prostaglandin glyceryl esters (PG-Gs).
29722963	2	20	theme	esters	459:464	arg1	esters					459:464	prostaglandin glyceryl esters	436:464	prostaglandin glyceryl esters (PG-Gs)	436:472	COX-2 also inactivates the endogenous cannabinoid (eCB) 2-arachidonoylglycerol (2-AG) via oxygenation of its arachidonic acid backbone to form a variety of prostaglandin glyceryl esters (PG-Gs).
29722963	4	21	theme	mice	752:755	arg1	brain					711:715	the brain	707:715	the brain of transgenic COX-2-overexpressing mice and mice treated with lipopolysaccharide to form multiple species of PG-Gs that are detectable only when monoacylglycerol lipase is concomitantly blocked	707:909	Here we show that 2-AG is metabolized in the brain of transgenic COX-2-overexpressing mice and mice treated with lipopolysaccharide to form multiple species of PG-Gs that are detectable only when monoacylglycerol lipase is concomitantly blocked.
29722963	2	22	theme	eCB	331:333	arg1	2-AG					360:363	2-AG	360:363	2-AG	360:363	COX-2 also inactivates the endogenous cannabinoid (eCB) 2-arachidonoylglycerol (2-AG) via oxygenation of its arachidonic acid backbone to form a variety of prostaglandin glyceryl esters (PG-Gs).
29722963	2	22	theme	eCB	331:333	arg1	2-arachidonoylglycerol					336:357	the endogenous cannabinoid (eCB) 2-arachidonoylglycerol	303:357	the endogenous cannabinoid (eCB) 2-arachidonoylglycerol (2-AG)	303:364	COX-2 also inactivates the endogenous cannabinoid (eCB) 2-arachidonoylglycerol (2-AG) via oxygenation of its arachidonic acid backbone to form a variety of prostaglandin glyceryl esters (PG-Gs).
29722963	2	23	theme	prostaglandin	436:448	arg1	PG-Gs					467:471	PG-Gs	467:471	PG-Gs	467:471	COX-2 also inactivates the endogenous cannabinoid (eCB) 2-arachidonoylglycerol (2-AG) via oxygenation of its arachidonic acid backbone to form a variety of prostaglandin glyceryl esters (PG-Gs).
29722963	2	23	theme	prostaglandin	436:448	arg1	esters					459:464	prostaglandin glyceryl esters	436:464	prostaglandin glyceryl esters (PG-Gs)	436:472	COX-2 also inactivates the endogenous cannabinoid (eCB) 2-arachidonoylglycerol (2-AG) via oxygenation of its arachidonic acid backbone to form a variety of prostaglandin glyceryl esters (PG-Gs).
29722963	2	24	theme	glyceryl	450:457	arg1	PG-Gs					467:471	PG-Gs	467:471	PG-Gs	467:471	COX-2 also inactivates the endogenous cannabinoid (eCB) 2-arachidonoylglycerol (2-AG) via oxygenation of its arachidonic acid backbone to form a variety of prostaglandin glyceryl esters (PG-Gs).
29722963	2	24	theme	glyceryl	450:457	arg1	esters					459:464	prostaglandin glyceryl esters	436:464	prostaglandin glyceryl esters (PG-Gs)	436:472	COX-2 also inactivates the endogenous cannabinoid (eCB) 2-arachidonoylglycerol (2-AG) via oxygenation of its arachidonic acid backbone to form a variety of prostaglandin glyceryl esters (PG-Gs).
29722963	4	25	theme	mice	761:764	arg1	brain					711:715	the brain	707:715	the brain of transgenic COX-2-overexpressing mice and mice treated with lipopolysaccharide to form multiple species of PG-Gs that are detectable only when monoacylglycerol lipase is concomitantly blocked	707:909	Here we show that 2-AG is metabolized in the brain of transgenic COX-2-overexpressing mice and mice treated with lipopolysaccharide to form multiple species of PG-Gs that are detectable only when monoacylglycerol lipase is concomitantly blocked.
29722963	0	26	theme	Products	50:57	arg1	Detection					0:8	Detection	0:8	Detection of Cyclooxygenase-2-Derived Oxygenation Products of the Endogenous Cannabinoid 2-Arachidonoylglycerol in Mouse Brain.	0:126	Detection of Cyclooxygenase-2-Derived Oxygenation Products of the Endogenous Cannabinoid 2-Arachidonoylglycerol in Mouse Brain.
29722963	3	27	theme	neuronal	649:656	arg1	tissue					658:663	neuronal tissue	649:663	neuronal tissue	649:663	Although this oxygenation reaction is readily observed in vitro and in intact cells, detection of COX-2-derived 2-AG oxygenation products has not been previously reported in neuronal tissue.
29722963	0	28	theme	Oxygenation	38:48	arg1	Products					50:57	Cyclooxygenase-2-Derived Oxygenation Products	13:57	Cyclooxygenase-2-Derived Oxygenation Products of the Endogenous Cannabinoid 2-Arachidonoylglycerol	13:110	Detection of Cyclooxygenase-2-Derived Oxygenation Products of the Endogenous Cannabinoid 2-Arachidonoylglycerol in Mouse Brain.
29722963	1	29	theme	vascular	237:244	arg1	function					246:253	vascular function	237:253	vascular function	237:253	Cyclooxygenase-2 (COX-2) catalyzes the formation of prostaglandins, which are involved in immune regulation, vascular function, and synaptic signaling.
29722963	0	30	theme	Endogenous	66:75	arg1	2-Arachidonoylglycerol					89:110	the Endogenous Cannabinoid 2-Arachidonoylglycerol	62:110	the Endogenous Cannabinoid 2-Arachidonoylglycerol	62:110	Detection of Cyclooxygenase-2-Derived Oxygenation Products of the Endogenous Cannabinoid 2-Arachidonoylglycerol in Mouse Brain.
29722963	3	31	theme	oxygenation	592:602	arg1	products					604:611	COX-2-derived 2-AG oxygenation products	573:611	COX-2-derived 2-AG oxygenation products	573:611	Although this oxygenation reaction is readily observed in vitro and in intact cells, detection of COX-2-derived 2-AG oxygenation products has not been previously reported in neuronal tissue.
29722963	3	32	theme	products	604:611	arg1	detection					560:568	detection	560:568	detection of COX-2-derived 2-AG oxygenation products	560:611	Although this oxygenation reaction is readily observed in vitro and in intact cells, detection of COX-2-derived 2-AG oxygenation products has not been previously reported in neuronal tissue.
29722963	6	33	theme	functions	1162:1170	arg1	investigation					1127:1139	further investigation	1119:1139	further investigation of the physiological functions of PG-Gs	1119:1179	These data provide evidence that neuronal COX-2 is capable of oxygenating 2-AG to form a variety PG-Gs in vivo and support further investigation of the physiological functions of PG-Gs.
29722963	3	34	located	observed	521:528	arg1	vitro					533:537	vitro	533:537	vitro	533:537	Although this oxygenation reaction is readily observed in vitro and in intact cells, detection of COX-2-derived 2-AG oxygenation products has not been previously reported in neuronal tissue.
29722963	3	34	located	observed	521:528	arg2	reaction					501:508	this oxygenation reaction	484:508	this oxygenation reaction	484:508	Although this oxygenation reaction is readily observed in vitro and in intact cells, detection of COX-2-derived 2-AG oxygenation products has not been previously reported in neuronal tissue.
29722963	3	35	theme	oxygenation	489:499	arg1	reaction					501:508	this oxygenation reaction	484:508	this oxygenation reaction	484:508	Although this oxygenation reaction is readily observed in vitro and in intact cells, detection of COX-2-derived 2-AG oxygenation products has not been previously reported in neuronal tissue.
29722963	5	36	theme	COX-2	989:993	arg1	inhibition					975:984	acute pharmacological inhibition	953:984	acute pharmacological inhibition of COX-2	953:993	Formation of these PG-Gs is prevented by acute pharmacological inhibition of COX-2.
29722963	4	37	theme	multiple	806:813	arg1	species					815:821	multiple species	806:821	multiple species of PG-Gs that are detectable only when monoacylglycerol lipase is concomitantly blocked	806:909	Here we show that 2-AG is metabolized in the brain of transgenic COX-2-overexpressing mice and mice treated with lipopolysaccharide to form multiple species of PG-Gs that are detectable only when monoacylglycerol lipase is concomitantly blocked.
29722963	4	38	theme	transgenic	720:729	arg1	mice					752:755	transgenic COX-2-overexpressing mice	720:755	transgenic COX-2-overexpressing mice	720:755	Here we show that 2-AG is metabolized in the brain of transgenic COX-2-overexpressing mice and mice treated with lipopolysaccharide to form multiple species of PG-Gs that are detectable only when monoacylglycerol lipase is concomitantly blocked.
29722963	4	39	theme	COX-2-overexpressing	731:750	arg1	mice					752:755	transgenic COX-2-overexpressing mice	720:755	transgenic COX-2-overexpressing mice	720:755	Here we show that 2-AG is metabolized in the brain of transgenic COX-2-overexpressing mice and mice treated with lipopolysaccharide to form multiple species of PG-Gs that are detectable only when monoacylglycerol lipase is concomitantly blocked.
29722963	0	40	theme	2-Arachidonoylglycerol	89:110	arg1	Products					50:57	Cyclooxygenase-2-Derived Oxygenation Products	13:57	Cyclooxygenase-2-Derived Oxygenation Products of the Endogenous Cannabinoid 2-Arachidonoylglycerol	13:110	Detection of Cyclooxygenase-2-Derived Oxygenation Products of the Endogenous Cannabinoid 2-Arachidonoylglycerol in Mouse Brain.
29722963	4	41	theme	PG-Gs	826:830	arg1	species					815:821	multiple species	806:821	multiple species of PG-Gs that are detectable only when monoacylglycerol lipase is concomitantly blocked	806:909	Here we show that 2-AG is metabolized in the brain of transgenic COX-2-overexpressing mice and mice treated with lipopolysaccharide to form multiple species of PG-Gs that are detectable only when monoacylglycerol lipase is concomitantly blocked.
29722963	1	42	theme	immune	218:223	arg1	regulation					225:234	immune regulation	218:234	immune regulation	218:234	Cyclooxygenase-2 (COX-2) catalyzes the formation of prostaglandins, which are involved in immune regulation, vascular function, and synaptic signaling.
29722963	2	43	theme	arachidonic	389:399	arg1	backbone					406:413	its arachidonic acid backbone	385:413	its arachidonic acid backbone to form a variety of prostaglandin glyceryl esters (PG-Gs)	385:472	COX-2 also inactivates the endogenous cannabinoid (eCB) 2-arachidonoylglycerol (2-AG) via oxygenation of its arachidonic acid backbone to form a variety of prostaglandin glyceryl esters (PG-Gs).
29722963	0	44	theme	Cannabinoid	77:87	arg1	2-Arachidonoylglycerol					89:110	the Endogenous Cannabinoid 2-Arachidonoylglycerol	62:110	the Endogenous Cannabinoid 2-Arachidonoylglycerol	62:110	Detection of Cyclooxygenase-2-Derived Oxygenation Products of the Endogenous Cannabinoid 2-Arachidonoylglycerol in Mouse Brain.
29722963	0	45	theme	Cyclooxygenase-2-Derived	13:36	arg1	Products					50:57	Cyclooxygenase-2-Derived Oxygenation Products	13:57	Cyclooxygenase-2-Derived Oxygenation Products of the Endogenous Cannabinoid 2-Arachidonoylglycerol	13:110	Detection of Cyclooxygenase-2-Derived Oxygenation Products of the Endogenous Cannabinoid 2-Arachidonoylglycerol in Mouse Brain.
29722963	1	46	theme	synaptic	260:267	arg1	signaling					269:277	synaptic signaling	260:277	synaptic signaling	260:277	Cyclooxygenase-2 (COX-2) catalyzes the formation of prostaglandins, which are involved in immune regulation, vascular function, and synaptic signaling.
30028280	5	0	theme	similarity	534:543	arg1	levels					501:506	low levels	497:506	low levels of 16S rRNA gene sequence similarity	497:543	Strain T8T shared low levels of 16S rRNA gene sequence similarity (<94 %) to members of other genera in the family Bacillaceae and was most closely related to Bacillus composti SgZ-9T (93.3 % sequence similarity).
30028280	2	1	theme	bacterial	74:82	arg1	strain					84:89	A novel bacterial strain	66:89	A novel bacterial strain	66:89	A novel bacterial strain, designated T8T, isolated from ripened Pu'er tea, was investigated by using a polyphasic taxonomic approach.
30028280	5	2	theme	%	669:669	arg1	similarity					680:689	93.3 % sequence similarity	664:689	93.3 % sequence similarity	664:689	Strain T8T shared low levels of 16S rRNA gene sequence similarity (<94 %) to members of other genera in the family Bacillaceae and was most closely related to Bacillus composti SgZ-9T (93.3 % sequence similarity).
30028280	5	2	theme	%	669:669	arg1	SgZ-9T					656:661	Bacillus composti SgZ-9T	638:661	Bacillus composti SgZ-9T (93.3 % sequence similarity)	638:690	Strain T8T shared low levels of 16S rRNA gene sequence similarity (<94 %) to members of other genera in the family Bacillaceae and was most closely related to Bacillus composti SgZ-9T (93.3 % sequence similarity).
30028280	5	3	theme	Strain	479:484	arg1	T8T					486:488	Strain T8T	479:488	Strain T8T	479:488	Strain T8T shared low levels of 16S rRNA gene sequence similarity (<94 %) to members of other genera in the family Bacillaceae and was most closely related to Bacillus composti SgZ-9T (93.3 % sequence similarity).
30028280	7	4	dep	iso-C15 	789:796	arg1	iso-C16 					805:812	iso-C16 	805:812	iso-C16 	805:812	The major fatty acids (>10 %) of strain T8T were iso-C15 : 0 and iso-C16 : 0.
30028280	7	4	dep	iso-C15 	789:796	arg1	 0					798:799	 0	798:799	 0	798:799	The major fatty acids (>10 %) of strain T8T were iso-C15 : 0 and iso-C16 : 0.
30028280	7	4	dep	iso-C15 	789:796	arg1	 0					814:815	 0	814:815	 0	814:815	The major fatty acids (>10 %) of strain T8T were iso-C15 : 0 and iso-C16 : 0.
30028280	4	5	theme	independent	432:442	arg1	taxon					444:448	an independent taxon	429:448	an independent taxon separated from other genera	429:476	Phylogenetic analysis of 16S rRNA gene sequences revealed the strain belonged to the family Bacillaceae in the class Bacilli and represented an independent taxon separated from other genera.
30028280	2	6	theme	novel	68:72	arg1	strain					84:89	A novel bacterial strain	66:89	A novel bacterial strain	66:89	A novel bacterial strain, designated T8T, isolated from ripened Pu'er tea, was investigated by using a polyphasic taxonomic approach.
30028280	4	7	theme	family	373:378	arg1	Bacillaceae					380:390	the family Bacillaceae	369:390	the family Bacillaceae in the class Bacilli	369:411	Phylogenetic analysis of 16S rRNA gene sequences revealed the strain belonged to the family Bacillaceae in the class Bacilli and represented an independent taxon separated from other genera.
30028280	5	8	theme	sequence	525:532	arg1	similarity					534:543	16S rRNA gene sequence similarity	511:543	16S rRNA gene sequence similarity	511:543	Strain T8T shared low levels of 16S rRNA gene sequence similarity (<94 %) to members of other genera in the family Bacillaceae and was most closely related to Bacillus composti SgZ-9T (93.3 % sequence similarity).
30028280	8	9	theme	cell-wall	835:843	arg1	peptidoglycan					854:866	a cell-wall type A1γ peptidoglycan	833:866	a cell-wall type A1γ peptidoglycan with meso-diaminopimelic acid	833:896	The strain had a cell-wall type A1γ peptidoglycan with meso-diaminopimelic acid as the diagnostic diamino acid.
30028280	8	9	theme	cell-wall	835:843	arg1	acid					924:927	the diagnostic diamino acid	901:927	the diagnostic diamino acid	901:927	The strain had a cell-wall type A1γ peptidoglycan with meso-diaminopimelic acid as the diagnostic diamino acid.
30028280	8	10	contain	had	829:831	arg1	strain					822:827	The strain	818:827	The strain	818:827	The strain had a cell-wall type A1γ peptidoglycan with meso-diaminopimelic acid as the diagnostic diamino acid.
30028280	8	10	contain	had	829:831	arg2	peptidoglycan					854:866	a cell-wall type A1γ peptidoglycan	833:866	a cell-wall type A1γ peptidoglycan with meso-diaminopimelic acid	833:896	The strain had a cell-wall type A1γ peptidoglycan with meso-diaminopimelic acid as the diagnostic diamino acid.
30028280	8	10	contain	had	829:831	arg2	acid					924:927	the diagnostic diamino acid	901:927	the diagnostic diamino acid	901:927	The strain had a cell-wall type A1γ peptidoglycan with meso-diaminopimelic acid as the diagnostic diamino acid.
30028280	5	11	theme	genera	573:578	arg1	members					556:562	members	556:562	members of other genera in the family Bacillaceae	556:604	Strain T8T shared low levels of 16S rRNA gene sequence similarity (<94 %) to members of other genera in the family Bacillaceae and was most closely related to Bacillus composti SgZ-9T (93.3 % sequence similarity).
30028280	4	12	theme	sequences	327:335	arg1	analysis					301:308	Phylogenetic analysis	288:308	Phylogenetic analysis of 16S rRNA gene sequences	288:335	Phylogenetic analysis of 16S rRNA gene sequences revealed the strain belonged to the family Bacillaceae in the class Bacilli and represented an independent taxon separated from other genera.
30028280	10	13	theme	polar	1028:1032	arg1	lipids					1034:1039	The predominant polar lipids	1012:1039	The predominant polar lipids	1012:1039	The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine and six unidentified phospholipids.
30028280	10	13	theme	polar	1028:1032	arg1	diphosphatidylglycerol					1046:1067	diphosphatidylglycerol	1046:1067	diphosphatidylglycerol	1046:1067	The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine and six unidentified phospholipids.
30028280	5	14	theme	Bacillus	638:645	arg1	similarity					680:689	93.3 % sequence similarity	664:689	93.3 % sequence similarity	664:689	Strain T8T shared low levels of 16S rRNA gene sequence similarity (<94 %) to members of other genera in the family Bacillaceae and was most closely related to Bacillus composti SgZ-9T (93.3 % sequence similarity).
30028280	5	14	theme	Bacillus	638:645	arg1	SgZ-9T					656:661	Bacillus composti SgZ-9T	638:661	Bacillus composti SgZ-9T (93.3 % sequence similarity)	638:690	Strain T8T shared low levels of 16S rRNA gene sequence similarity (<94 %) to members of other genera in the family Bacillaceae and was most closely related to Bacillus composti SgZ-9T (93.3 % sequence similarity).
30028280	11	15	theme	family	1281:1286	arg1	Bacillaceae					1288:1298	the family Bacillaceae	1277:1298	the family Bacillaceae	1277:1298	On the basis of the polyphasic evidence presented, strain T8T is considered to represent a novel genus and species in the family Bacillaceae, for which we propose the name Pueribacillus theae gen. nov., sp.
30028280	8	16	theme	meso-diaminopimelic	873:891	arg1	acid					893:896	meso-diaminopimelic acid	873:896	meso-diaminopimelic acid	873:896	The strain had a cell-wall type A1γ peptidoglycan with meso-diaminopimelic acid as the diagnostic diamino acid.
30028280	11	17	theme	Pueribacillus	1331:1343	arg1	nov.					1356:1359	Pueribacillus theae gen. nov.	1331:1359	the name Pueribacillus theae gen. nov.	1322:1359	On the basis of the polyphasic evidence presented, strain T8T is considered to represent a novel genus and species in the family Bacillaceae, for which we propose the name Pueribacillus theae gen. nov., sp.
30028280	7	18	theme	major	744:748	arg1	acids					756:760	The major fatty acids	740:760	The major fatty acids (>10 %) of strain T8T	740:782	The major fatty acids (>10 %) of strain T8T were iso-C15 : 0 and iso-C16 : 0.
30028280	7	18	theme	major	744:748	arg1	%					767:767	>10 %	763:767	>10 %	763:767	The major fatty acids (>10 %) of strain T8T were iso-C15 : 0 and iso-C16 : 0.
30028280	7	18	theme	major	744:748	arg1	iso-C15 					789:796	iso-C15 	789:796	iso-C15 	789:796	The major fatty acids (>10 %) of strain T8T were iso-C15 : 0 and iso-C16 : 0.
30028280	8	19	with	peptidoglycan	854:866	arg1	acid					893:896	meso-diaminopimelic acid	873:896	meso-diaminopimelic acid	873:896	The strain had a cell-wall type A1γ peptidoglycan with meso-diaminopimelic acid as the diagnostic diamino acid.
30028280	5	20	theme	composti	647:654	arg1	similarity					680:689	93.3 % sequence similarity	664:689	93.3 % sequence similarity	664:689	Strain T8T shared low levels of 16S rRNA gene sequence similarity (<94 %) to members of other genera in the family Bacillaceae and was most closely related to Bacillus composti SgZ-9T (93.3 % sequence similarity).
30028280	5	20	theme	composti	647:654	arg1	SgZ-9T					656:661	Bacillus composti SgZ-9T	638:661	Bacillus composti SgZ-9T (93.3 % sequence similarity)	638:690	Strain T8T shared low levels of 16S rRNA gene sequence similarity (<94 %) to members of other genera in the family Bacillaceae and was most closely related to Bacillus composti SgZ-9T (93.3 % sequence similarity).
30028280	6	21	theme	DNA	697:699	arg1	G+C content					701:711	The DNA G+C content	693:711	The DNA G+C content of strain T8T	693:725	The DNA G+C content of strain T8T was 40 mol%.
30028280	6	21	theme	DNA	697:699	arg1	%					737:737	40 mol%	731:737	40 mol%	731:737	The DNA G+C content of strain T8T was 40 mol%.
30028280	7	22	theme	fatty	750:754	arg1	acids					756:760	The major fatty acids	740:760	The major fatty acids (>10 %) of strain T8T	740:782	The major fatty acids (>10 %) of strain T8T were iso-C15 : 0 and iso-C16 : 0.
30028280	7	22	theme	fatty	750:754	arg1	%					767:767	>10 %	763:767	>10 %	763:767	The major fatty acids (>10 %) of strain T8T were iso-C15 : 0 and iso-C16 : 0.
30028280	7	22	theme	fatty	750:754	arg1	iso-C15 					789:796	iso-C15 	789:796	iso-C15 	789:796	The major fatty acids (>10 %) of strain T8T were iso-C15 : 0 and iso-C16 : 0.
30028280	11	23	from	genus	1256:1260	arg1	Bacillaceae					1288:1298	the family Bacillaceae	1277:1298	the family Bacillaceae	1277:1298	On the basis of the polyphasic evidence presented, strain T8T is considered to represent a novel genus and species in the family Bacillaceae, for which we propose the name Pueribacillus theae gen. nov., sp.
30028280	4	24	theme	16S	313:315	arg1	sequences					327:335	16S rRNA gene sequences	313:335	16S rRNA gene sequences	313:335	Phylogenetic analysis of 16S rRNA gene sequences revealed the strain belonged to the family Bacillaceae in the class Bacilli and represented an independent taxon separated from other genera.
30028280	13	25	theme	=CGMCC	1395:1400	arg1	T8T					1390:1392	T8T	1390:1392	T8T (=CGMCC 1.15924T=KCTC 333888T)	1390:1423	The type strain is T8T (=CGMCC 1.15924T=KCTC 333888T).
30028280	13	25	theme	=CGMCC	1395:1400	arg1	333888T					1416:1422	=CGMCC 1.15924T=KCTC 333888T	1395:1422	=CGMCC 1.15924T=KCTC 333888T	1395:1422	The type strain is T8T (=CGMCC 1.15924T=KCTC 333888T).
30028280	11	26	theme	novel	1250:1254	arg1	genus					1256:1260	a novel genus	1248:1260	a novel genus	1248:1260	On the basis of the polyphasic evidence presented, strain T8T is considered to represent a novel genus and species in the family Bacillaceae, for which we propose the name Pueribacillus theae gen. nov., sp.
30028280	0	27	theme	theae	14:18	arg1	nov.					25:28	Pueribacillus theae gen. nov.	0:28	Pueribacillus theae gen. nov.	0:28	Pueribacillus theae gen. nov., sp.
30028280	5	28	theme	sequence	671:678	arg1	similarity					680:689	93.3 % sequence similarity	664:689	93.3 % sequence similarity	664:689	Strain T8T shared low levels of 16S rRNA gene sequence similarity (<94 %) to members of other genera in the family Bacillaceae and was most closely related to Bacillus composti SgZ-9T (93.3 % sequence similarity).
30028280	5	28	theme	sequence	671:678	arg1	SgZ-9T					656:661	Bacillus composti SgZ-9T	638:661	Bacillus composti SgZ-9T (93.3 % sequence similarity)	638:690	Strain T8T shared low levels of 16S rRNA gene sequence similarity (<94 %) to members of other genera in the family Bacillaceae and was most closely related to Bacillus composti SgZ-9T (93.3 % sequence similarity).
30028280	8	29	theme	A1γ	850:852	arg1	peptidoglycan					854:866	a cell-wall type A1γ peptidoglycan	833:866	a cell-wall type A1γ peptidoglycan with meso-diaminopimelic acid	833:896	The strain had a cell-wall type A1γ peptidoglycan with meso-diaminopimelic acid as the diagnostic diamino acid.
30028280	8	29	theme	A1γ	850:852	arg1	acid					924:927	the diagnostic diamino acid	901:927	the diagnostic diamino acid	901:927	The strain had a cell-wall type A1γ peptidoglycan with meso-diaminopimelic acid as the diagnostic diamino acid.
30028280	5	30	theme	16S	511:513	arg1	similarity					534:543	16S rRNA gene sequence similarity	511:543	16S rRNA gene sequence similarity	511:543	Strain T8T shared low levels of 16S rRNA gene sequence similarity (<94 %) to members of other genera in the family Bacillaceae and was most closely related to Bacillus composti SgZ-9T (93.3 % sequence similarity).
30028280	5	31	dep	shared	490:495	arg1	%					550:550	<94 %	546:550	<94 %	546:550	Strain T8T shared low levels of 16S rRNA gene sequence similarity (<94 %) to members of other genera in the family Bacillaceae and was most closely related to Bacillus composti SgZ-9T (93.3 % sequence similarity).
30028280	3	32	with	aerobic	237:243	arg1	flagella					278:285	flagella	278:285	flagella	278:285	Cells stained Gram-positive and were aerobic, sporogenous and rod-shaped with flagella.
30028280	0	33	theme	Pueribacillus	0:12	arg1	nov.					25:28	Pueribacillus theae gen. nov.	0:28	Pueribacillus theae gen. nov.	0:28	Pueribacillus theae gen. nov., sp.
30028280	11	34	theme	gen.	1351:1354	arg1	nov.					1356:1359	Pueribacillus theae gen. nov.	1331:1359	the name Pueribacillus theae gen. nov.	1322:1359	On the basis of the polyphasic evidence presented, strain T8T is considered to represent a novel genus and species in the family Bacillaceae, for which we propose the name Pueribacillus theae gen. nov., sp.
30028280	8	35	theme	diagnostic	905:914	arg1	acid					924:927	the diagnostic diamino acid	901:927	the diagnostic diamino acid	901:927	The strain had a cell-wall type A1γ peptidoglycan with meso-diaminopimelic acid as the diagnostic diamino acid.
30028280	8	35	theme	diagnostic	905:914	arg1	peptidoglycan					854:866	a cell-wall type A1γ peptidoglycan	833:866	a cell-wall type A1γ peptidoglycan with meso-diaminopimelic acid	833:896	The strain had a cell-wall type A1γ peptidoglycan with meso-diaminopimelic acid as the diagnostic diamino acid.
30028280	4	36	theme	gene	322:325	arg1	sequences					327:335	16S rRNA gene sequences	313:335	16S rRNA gene sequences	313:335	Phylogenetic analysis of 16S rRNA gene sequences revealed the strain belonged to the family Bacillaceae in the class Bacilli and represented an independent taxon separated from other genera.
30028280	1	37	theme	er	58:59	arg1	tea					61:63	Pu'er tea	55:63	Pu'er tea	55:63	nov., isolated from Pu'er tea.
30028280	11	38	dep	name	1326:1329	arg1	nov.					1356:1359	Pueribacillus theae gen. nov.	1331:1359	the name Pueribacillus theae gen. nov.	1322:1359	On the basis of the polyphasic evidence presented, strain T8T is considered to represent a novel genus and species in the family Bacillaceae, for which we propose the name Pueribacillus theae gen. nov., sp.
30028280	5	39	theme	other	567:571	arg1	genera					573:578	other genera	567:578	other genera in the family Bacillaceae	567:604	Strain T8T shared low levels of 16S rRNA gene sequence similarity (<94 %) to members of other genera in the family Bacillaceae and was most closely related to Bacillus composti SgZ-9T (93.3 % sequence similarity).
30028280	5	40	theme	rRNA	515:518	arg1	similarity					534:543	16S rRNA gene sequence similarity	511:543	16S rRNA gene sequence similarity	511:543	Strain T8T shared low levels of 16S rRNA gene sequence similarity (<94 %) to members of other genera in the family Bacillaceae and was most closely related to Bacillus composti SgZ-9T (93.3 % sequence similarity).
30028280	4	41	theme	rRNA	317:320	arg1	sequences					327:335	16S rRNA gene sequences	313:335	16S rRNA gene sequences	313:335	Phylogenetic analysis of 16S rRNA gene sequences revealed the strain belonged to the family Bacillaceae in the class Bacilli and represented an independent taxon separated from other genera.
30028280	13	42	theme	type	1375:1378	arg1	T8T					1390:1392	T8T	1390:1392	T8T (=CGMCC 1.15924T=KCTC 333888T)	1390:1423	The type strain is T8T (=CGMCC 1.15924T=KCTC 333888T).
30028280	13	42	theme	type	1375:1378	arg1	strain					1380:1385	The type strain	1371:1385	The type strain	1371:1385	The type strain is T8T (=CGMCC 1.15924T=KCTC 333888T).
30028280	6	43	theme	strain	716:721	arg1	T8T					723:725	strain T8T	716:725	strain T8T	716:725	The DNA G+C content of strain T8T was 40 mol%.
30028280	0	44	theme	gen.	20:23	arg1	nov.					25:28	Pueribacillus theae gen. nov.	0:28	Pueribacillus theae gen. nov.	0:28	Pueribacillus theae gen. nov., sp.
30028280	2	45	theme	Pu	130:131	arg1	tea					136:138	ripened Pu'er tea	122:138	ripened Pu'er tea	122:138	A novel bacterial strain, designated T8T, isolated from ripened Pu'er tea, was investigated by using a polyphasic taxonomic approach.
30028280	11	46	theme	polyphasic	1179:1188	arg1	evidence					1190:1197	the polyphasic evidence	1175:1197	the polyphasic evidence presented	1175:1207	On the basis of the polyphasic evidence presented, strain T8T is considered to represent a novel genus and species in the family Bacillaceae, for which we propose the name Pueribacillus theae gen. nov., sp.
30028280	11	47	dep	evidence	1190:1197	arg1	the					1162:1164	the	1162:1164	the	1162:1164	On the basis of the polyphasic evidence presented, strain T8T is considered to represent a novel genus and species in the family Bacillaceae, for which we propose the name Pueribacillus theae gen. nov., sp.
30028280	11	47	dep	evidence	1190:1197	arg1	basis					1166:1170	basis	1166:1170	basis	1166:1170	On the basis of the polyphasic evidence presented, strain T8T is considered to represent a novel genus and species in the family Bacillaceae, for which we propose the name Pueribacillus theae gen. nov., sp.
30028280	2	48	theme	ripened	122:128	arg1	tea					136:138	ripened Pu'er tea	122:138	ripened Pu'er tea	122:138	A novel bacterial strain, designated T8T, isolated from ripened Pu'er tea, was investigated by using a polyphasic taxonomic approach.
30028280	11	49	from	species	1266:1272	arg1	Bacillaceae					1288:1298	the family Bacillaceae	1277:1298	the family Bacillaceae	1277:1298	On the basis of the polyphasic evidence presented, strain T8T is considered to represent a novel genus and species in the family Bacillaceae, for which we propose the name Pueribacillus theae gen. nov., sp.
30028280	2	50	attach	isolated	108:115	arg1	tea					136:138	ripened Pu'er tea	122:138	ripened Pu'er tea	122:138	A novel bacterial strain, designated T8T, isolated from ripened Pu'er tea, was investigated by using a polyphasic taxonomic approach.
30028280	2	50	attach	isolated	108:115	arg2	strain					84:89	A novel bacterial strain	66:89	A novel bacterial strain	66:89	A novel bacterial strain, designated T8T, isolated from ripened Pu'er tea, was investigated by using a polyphasic taxonomic approach.
30028280	10	51	theme	predominant	1016:1026	arg1	lipids					1034:1039	The predominant polar lipids	1012:1039	The predominant polar lipids	1012:1039	The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine and six unidentified phospholipids.
30028280	10	51	theme	predominant	1016:1026	arg1	diphosphatidylglycerol					1046:1067	diphosphatidylglycerol	1046:1067	diphosphatidylglycerol	1046:1067	The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine and six unidentified phospholipids.
30028280	5	52	from	genera	573:578	arg1	Bacillaceae					594:604	the family Bacillaceae	583:604	the family Bacillaceae	583:604	Strain T8T shared low levels of 16S rRNA gene sequence similarity (<94 %) to members of other genera in the family Bacillaceae and was most closely related to Bacillus composti SgZ-9T (93.3 % sequence similarity).
30028280	4	53	from	Bacillaceae	380:390	arg1	Bacilli					405:411	the class Bacilli	395:411	the class Bacilli	395:411	Phylogenetic analysis of 16S rRNA gene sequences revealed the strain belonged to the family Bacillaceae in the class Bacilli and represented an independent taxon separated from other genera.
30028280	4	54	theme	class	399:403	arg1	Bacilli					405:411	the class Bacilli	395:411	the class Bacilli	395:411	Phylogenetic analysis of 16S rRNA gene sequences revealed the strain belonged to the family Bacillaceae in the class Bacilli and represented an independent taxon separated from other genera.
30028280	8	55	theme	type	845:848	arg1	peptidoglycan					854:866	a cell-wall type A1γ peptidoglycan	833:866	a cell-wall type A1γ peptidoglycan with meso-diaminopimelic acid	833:896	The strain had a cell-wall type A1γ peptidoglycan with meso-diaminopimelic acid as the diagnostic diamino acid.
30028280	8	55	theme	type	845:848	arg1	acid					924:927	the diagnostic diamino acid	901:927	the diagnostic diamino acid	901:927	The strain had a cell-wall type A1γ peptidoglycan with meso-diaminopimelic acid as the diagnostic diamino acid.
30028280	4	56	theme	other	465:469	arg1	genera					471:476	other genera	465:476	other genera	465:476	Phylogenetic analysis of 16S rRNA gene sequences revealed the strain belonged to the family Bacillaceae in the class Bacilli and represented an independent taxon separated from other genera.
30028280	13	57	theme	1.15924T=KCTC	1402:1414	arg1	T8T					1390:1392	T8T	1390:1392	T8T (=CGMCC 1.15924T=KCTC 333888T)	1390:1423	The type strain is T8T (=CGMCC 1.15924T=KCTC 333888T).
30028280	13	57	theme	1.15924T=KCTC	1402:1414	arg1	333888T					1416:1422	=CGMCC 1.15924T=KCTC 333888T	1395:1422	=CGMCC 1.15924T=KCTC 333888T	1395:1422	The type strain is T8T (=CGMCC 1.15924T=KCTC 333888T).
30028280	5	58	from	members	556:562	arg1	Bacillaceae					594:604	the family Bacillaceae	583:604	the family Bacillaceae	583:604	Strain T8T shared low levels of 16S rRNA gene sequence similarity (<94 %) to members of other genera in the family Bacillaceae and was most closely related to Bacillus composti SgZ-9T (93.3 % sequence similarity).
30028280	0	59	dep	sp	31:32	arg1	nov.					25:28	Pueribacillus theae gen. nov.	0:28	Pueribacillus theae gen. nov.	0:28	Pueribacillus theae gen. nov., sp.
30028280	9	60	theme	isoprenoid	991:1000	arg1	MK-7					930:933	MK-7	930:933	MK-7	930:933	MK-7 (62 %), MK-6 (31 %) and MK-8 (7 %) were detected as the isoprenoid quinones.
30028280	9	60	theme	isoprenoid	991:1000	arg1	quinones					1002:1009	the isoprenoid quinones	987:1009	the isoprenoid quinones	987:1009	MK-7 (62 %), MK-6 (31 %) and MK-8 (7 %) were detected as the isoprenoid quinones.
30028280	9	60	theme	isoprenoid	991:1000	arg1	MK-8					959:962	MK-8	959:962	MK-8	959:962	MK-7 (62 %), MK-6 (31 %) and MK-8 (7 %) were detected as the isoprenoid quinones.
30028280	9	60	theme	isoprenoid	991:1000	arg1	MK-6					943:946	MK-6	943:946	MK-6	943:946	MK-7 (62 %), MK-6 (31 %) and MK-8 (7 %) were detected as the isoprenoid quinones.
30028280	5	61	theme	family	587:592	arg1	Bacillaceae					594:604	the family Bacillaceae	583:604	the family Bacillaceae	583:604	Strain T8T shared low levels of 16S rRNA gene sequence similarity (<94 %) to members of other genera in the family Bacillaceae and was most closely related to Bacillus composti SgZ-9T (93.3 % sequence similarity).
30028280	5	62	theme	low	497:499	arg1	levels					501:506	low levels	497:506	low levels of 16S rRNA gene sequence similarity	497:543	Strain T8T shared low levels of 16S rRNA gene sequence similarity (<94 %) to members of other genera in the family Bacillaceae and was most closely related to Bacillus composti SgZ-9T (93.3 % sequence similarity).
30028280	7	63	theme	strain	773:778	arg1	T8T					780:782	strain T8T	773:782	strain T8T	773:782	The major fatty acids (>10 %) of strain T8T were iso-C15 : 0 and iso-C16 : 0.
30028280	5	64	theme	gene	520:523	arg1	similarity					534:543	16S rRNA gene sequence similarity	511:543	16S rRNA gene sequence similarity	511:543	Strain T8T shared low levels of 16S rRNA gene sequence similarity (<94 %) to members of other genera in the family Bacillaceae and was most closely related to Bacillus composti SgZ-9T (93.3 % sequence similarity).
30028280	6	65	theme	40 mol	731:736	arg1	G+C content					701:711	The DNA G+C content	693:711	The DNA G+C content of strain T8T	693:725	The DNA G+C content of strain T8T was 40 mol%.
30028280	6	65	theme	40 mol	731:736	arg1	%					737:737	40 mol%	731:737	40 mol%	731:737	The DNA G+C content of strain T8T was 40 mol%.
30028280	4	66	theme	Phylogenetic	288:299	arg1	analysis					301:308	Phylogenetic analysis	288:308	Phylogenetic analysis of 16S rRNA gene sequences	288:335	Phylogenetic analysis of 16S rRNA gene sequences revealed the strain belonged to the family Bacillaceae in the class Bacilli and represented an independent taxon separated from other genera.
30028280	7	67	theme	T8T	780:782	arg1	acids					756:760	The major fatty acids	740:760	The major fatty acids (>10 %) of strain T8T	740:782	The major fatty acids (>10 %) of strain T8T were iso-C15 : 0 and iso-C16 : 0.
30028280	7	67	theme	T8T	780:782	arg1	%					767:767	>10 %	763:767	>10 %	763:767	The major fatty acids (>10 %) of strain T8T were iso-C15 : 0 and iso-C16 : 0.
30028280	7	67	theme	T8T	780:782	arg1	iso-C15 					789:796	iso-C15 	789:796	iso-C15 	789:796	The major fatty acids (>10 %) of strain T8T were iso-C15 : 0 and iso-C16 : 0.
30028280	5	68	from	Bacillaceae	594:604	arg1	members					556:562	members	556:562	members of other genera in the family Bacillaceae	556:604	Strain T8T shared low levels of 16S rRNA gene sequence similarity (<94 %) to members of other genera in the family Bacillaceae and was most closely related to Bacillus composti SgZ-9T (93.3 % sequence similarity).
30028280	2	69	theme	er	133:134	arg1	tea					136:138	ripened Pu'er tea	122:138	ripened Pu'er tea	122:138	A novel bacterial strain, designated T8T, isolated from ripened Pu'er tea, was investigated by using a polyphasic taxonomic approach.
30028280	2	70	theme	taxonomic	180:188	arg1	approach					190:197	a polyphasic taxonomic approach	167:197	a polyphasic taxonomic approach	167:197	A novel bacterial strain, designated T8T, isolated from ripened Pu'er tea, was investigated by using a polyphasic taxonomic approach.
30028280	10	71	theme	unidentified	1131:1142	arg1	phospholipids					1144:1156	six unidentified phospholipids	1127:1156	six unidentified phospholipids	1127:1156	The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine and six unidentified phospholipids.
30028280	11	72	theme	strain	1210:1215	arg1	T8T					1217:1219	strain T8T	1210:1219	strain T8T	1210:1219	On the basis of the polyphasic evidence presented, strain T8T is considered to represent a novel genus and species in the family Bacillaceae, for which we propose the name Pueribacillus theae gen. nov., sp.
30028280	6	73	theme	T8T	723:725	arg1	G+C content					701:711	The DNA G+C content	693:711	The DNA G+C content of strain T8T	693:725	The DNA G+C content of strain T8T was 40 mol%.
30028280	6	73	theme	T8T	723:725	arg1	%					737:737	40 mol%	731:737	40 mol%	731:737	The DNA G+C content of strain T8T was 40 mol%.
30028280	2	74	theme	polyphasic	169:178	arg1	approach					190:197	a polyphasic taxonomic approach	167:197	a polyphasic taxonomic approach	167:197	A novel bacterial strain, designated T8T, isolated from ripened Pu'er tea, was investigated by using a polyphasic taxonomic approach.
30028280	5	75	theme	93.3 	664:668	arg1	%					669:669	%	669:669	%	669:669	Strain T8T shared low levels of 16S rRNA gene sequence similarity (<94 %) to members of other genera in the family Bacillaceae and was most closely related to Bacillus composti SgZ-9T (93.3 % sequence similarity).
30028280	8	76	theme	diamino	916:922	arg1	acid					924:927	the diagnostic diamino acid	901:927	the diagnostic diamino acid	901:927	The strain had a cell-wall type A1γ peptidoglycan with meso-diaminopimelic acid as the diagnostic diamino acid.
30028280	8	76	theme	diamino	916:922	arg1	peptidoglycan					854:866	a cell-wall type A1γ peptidoglycan	833:866	a cell-wall type A1γ peptidoglycan with meso-diaminopimelic acid	833:896	The strain had a cell-wall type A1γ peptidoglycan with meso-diaminopimelic acid as the diagnostic diamino acid.
30028280	11	77	theme	theae	1345:1349	arg1	nov.					1356:1359	Pueribacillus theae gen. nov.	1331:1359	the name Pueribacillus theae gen. nov.	1322:1359	On the basis of the polyphasic evidence presented, strain T8T is considered to represent a novel genus and species in the family Bacillaceae, for which we propose the name Pueribacillus theae gen. nov., sp.
29966744	0	0	theme	lipid	85:89	arg1	nanoparticles					91:103	lipid nanoparticles	85:103	lipid nanoparticles	85:103	Mediterranean essential oils as precious matrix components and active ingredients of lipid nanoparticles.
29966744	9	1	dep	in	1701:1702	arg1	vitro					1704:1708	vitro	1704:1708	vitro	1704:1708	In vitro biological cell viability and anti-inflammatory activities were evaluated in Raw 264.7 cells (macrophage cell line), while in vitro antioxidant activity was checked by DPPH assay.
29966744	10	2	theme	anti-inflammatory	1927:1943	arg1	activity					1945:1952	a dose-dependent anti-inflammatory activity	1910:1952	a dose-dependent anti-inflammatory activity	1910:1952	Lavandula and Rosmarinus NLC were shown to be the most biocompatible formulations up to a concentration of 0.1% (v/v), whereas they were able to induce a dose-dependent anti-inflammatory activity in the order Lavandula > Rosmarinus ≥ Origanum.
29966744	10	3	dep	concentration	1848:1860	arg1	up					1840:1841	up	1840:1841	up	1840:1841	Lavandula and Rosmarinus NLC were shown to be the most biocompatible formulations up to a concentration of 0.1% (v/v), whereas they were able to induce a dose-dependent anti-inflammatory activity in the order Lavandula > Rosmarinus ≥ Origanum.
29966744	2	4	dep	volatility	492:501	arg1	i.e.					487:490	i.e.	487:490	i.e.	487:490	Their use in pharmaceutics is however compromised by their limited water solubility and low physicochemical stability (i.e. volatility, oxidation).
29966744	5	5	theme	solid	914:918	arg1	lipid					920:924	solid lipid	914:924	solid lipid	914:924	NLC composed of Softisan (as solid lipid) have been produced by phase inversion temperature (PIT) and high-pressure homogenization (HPH), using two different emulsifiers systems.
29966744	8	6	theme	composition	1511:1521	arg1	effect					1464:1469	the effect	1460:1469	the effect of the production method and surfactant composition on the long-term stability of EOs-loaded NLC	1460:1566	Accelerated stability studies have also been carried out to estimate the effect of the production method and surfactant composition on the long-term stability of EOs-loaded NLC.
29966744	3	7	dep	officinalis	691:701	arg1	Lavandula					707:715	Lavandula	707:715	Lavandula	707:715	In order to overcome these limitations, we aimed to develop nanostructured lipid carriers (NLC) as delivery systems for Mediterranean essential oils, in particular Rosmarinus officinalis L., Lavandula x intermedia "Sumian", Origanum vulgare subsp.
29966744	7	8	theme	Best	1206:1209	arg1	surfactants					1269:1279	surfactants	1269:1279	surfactants	1269:1279	Best NLC formulations were obtained with Kolliphor/Labrafil as surfactants, and using Rosmarinus, Lavandula and Origanum as essential oils (PDI between 0.126 and 0.141, Zave < 200 nm).
29966744	7	8	theme	Best	1206:1209	arg1	Origanum					1318:1325	Origanum	1318:1325	Origanum	1318:1325	Best NLC formulations were obtained with Kolliphor/Labrafil as surfactants, and using Rosmarinus, Lavandula and Origanum as essential oils (PDI between 0.126 and 0.141, Zave < 200 nm).
29966744	7	8	theme	Best	1206:1209	arg1	Lavandula					1304:1312	Lavandula	1304:1312	Lavandula	1304:1312	Best NLC formulations were obtained with Kolliphor/Labrafil as surfactants, and using Rosmarinus, Lavandula and Origanum as essential oils (PDI between 0.126 and 0.141, Zave < 200 nm).
29966744	7	8	theme	Best	1206:1209	arg1	formulations					1215:1226	Best NLC formulations	1206:1226	Best NLC formulations	1206:1226	Best NLC formulations were obtained with Kolliphor/Labrafil as surfactants, and using Rosmarinus, Lavandula and Origanum as essential oils (PDI between 0.126 and 0.141, Zave < 200 nm).
29966744	5	9	theme	high-pressure	987:999	arg1	homogenization					1001:1014	high-pressure homogenization	987:1014	high-pressure homogenization (HPH)	987:1020	NLC composed of Softisan (as solid lipid) have been produced by phase inversion temperature (PIT) and high-pressure homogenization (HPH), using two different emulsifiers systems.
29966744	5	9	theme	high-pressure	987:999	arg1	HPH					1017:1019	HPH	1017:1019	HPH	1017:1019	NLC composed of Softisan (as solid lipid) have been produced by phase inversion temperature (PIT) and high-pressure homogenization (HPH), using two different emulsifiers systems.
29966744	9	10	theme	macrophage	1672:1681	arg1	cells					1665:1669	Raw 264.7 cells	1655:1669	Raw 264.7 cells (macrophage cell line)	1655:1692	In vitro biological cell viability and anti-inflammatory activities were evaluated in Raw 264.7 cells (macrophage cell line), while in vitro antioxidant activity was checked by DPPH assay.
29966744	9	10	theme	macrophage	1672:1681	arg1	line					1688:1691	macrophage cell line	1672:1691	macrophage cell line	1672:1691	In vitro biological cell viability and anti-inflammatory activities were evaluated in Raw 264.7 cells (macrophage cell line), while in vitro antioxidant activity was checked by DPPH assay.
29966744	6	11	theme	chemical	1183:1190	arg1	interactions					1192:1203	chemical interactions	1183:1203	chemical interactions	1183:1203	Particles have been further characterized for their mean particle size, polydispersity, zeta potential, morphology and chemical interactions.
29966744	5	12	theme	inversion	955:963	arg1	PIT					978:980	PIT	978:980	PIT	978:980	NLC composed of Softisan (as solid lipid) have been produced by phase inversion temperature (PIT) and high-pressure homogenization (HPH), using two different emulsifiers systems.
29966744	5	12	theme	inversion	955:963	arg1	temperature					965:975	phase inversion temperature	949:975	phase inversion temperature (PIT)	949:981	NLC composed of Softisan (as solid lipid) have been produced by phase inversion temperature (PIT) and high-pressure homogenization (HPH), using two different emulsifiers systems.
29966744	0	13	theme	nanoparticles	91:103	arg1	components					48:57	precious matrix components	32:57	precious matrix components	32:57	Mediterranean essential oils as precious matrix components and active ingredients of lipid nanoparticles.
29966744	0	13	theme	nanoparticles	91:103	arg1	ingredients					70:80	active ingredients	63:80	active ingredients of lipid nanoparticles	63:103	Mediterranean essential oils as precious matrix components and active ingredients of lipid nanoparticles.
29966744	8	14	theme	long-term	1530:1538	arg1	stability					1540:1548	the long-term stability	1526:1548	the long-term stability of EOs-loaded NLC	1526:1566	Accelerated stability studies have also been carried out to estimate the effect of the production method and surfactant composition on the long-term stability of EOs-loaded NLC.
29966744	9	15	theme	cell	1683:1686	arg1	cells					1665:1669	Raw 264.7 cells	1655:1669	Raw 264.7 cells (macrophage cell line)	1655:1692	In vitro biological cell viability and anti-inflammatory activities were evaluated in Raw 264.7 cells (macrophage cell line), while in vitro antioxidant activity was checked by DPPH assay.
29966744	9	15	theme	cell	1683:1686	arg1	line					1688:1691	macrophage cell line	1672:1691	macrophage cell line	1672:1691	In vitro biological cell viability and anti-inflammatory activities were evaluated in Raw 264.7 cells (macrophage cell line), while in vitro antioxidant activity was checked by DPPH assay.
29966744	2	16	from	use	374:376	arg1	pharmaceutics					381:393	pharmaceutics	381:393	pharmaceutics	381:393	Their use in pharmaceutics is however compromised by their limited water solubility and low physicochemical stability (i.e. volatility, oxidation).
29966744	6	17	theme	mean	1116:1119	arg1	size					1130:1133	their mean particle size	1110:1133	their mean particle size	1110:1133	Particles have been further characterized for their mean particle size, polydispersity, zeta potential, morphology and chemical interactions.
29966744	3	18	theme	Origanum	740:747	arg1	carriers					597:604	nanostructured lipid carriers	576:604	nanostructured lipid carriers (NLC)	576:610	In order to overcome these limitations, we aimed to develop nanostructured lipid carriers (NLC) as delivery systems for Mediterranean essential oils, in particular Rosmarinus officinalis L., Lavandula x intermedia "Sumian", Origanum vulgare subsp.
29966744	3	18	theme	Origanum	740:747	arg1	subsp					757:761	Origanum vulgare subsp	740:761	Origanum vulgare subsp	740:761	In order to overcome these limitations, we aimed to develop nanostructured lipid carriers (NLC) as delivery systems for Mediterranean essential oils, in particular Rosmarinus officinalis L., Lavandula x intermedia "Sumian", Origanum vulgare subsp.
29966744	3	18	theme	Origanum	740:747	arg1	systems					624:630	delivery systems	615:630	delivery systems for Mediterranean essential oils	615:663	In order to overcome these limitations, we aimed to develop nanostructured lipid carriers (NLC) as delivery systems for Mediterranean essential oils, in particular Rosmarinus officinalis L., Lavandula x intermedia "Sumian", Origanum vulgare subsp.
29966744	4	19	theme	essential	792:800	arg1	oils					802:805	capitatus essential oils	782:805	capitatus essential oils	782:805	hirtum and Thymus capitatus essential oils, selected on the basis of their antioxidant and anti-inflammatory activities.
29966744	2	20	theme	limited	427:433	arg1	solubility					441:450	their limited water solubility	421:450	their limited water solubility	421:450	Their use in pharmaceutics is however compromised by their limited water solubility and low physicochemical stability (i.e. volatility, oxidation).
29966744	3	21	theme	Sumian	731:736	arg1	"					737:737	intermedia "Sumian"	719:737	intermedia "Sumian"	719:737	In order to overcome these limitations, we aimed to develop nanostructured lipid carriers (NLC) as delivery systems for Mediterranean essential oils, in particular Rosmarinus officinalis L., Lavandula x intermedia "Sumian", Origanum vulgare subsp.
29966744	4	22	theme	antioxidant	839:849	arg1	activities					873:882	their antioxidant and anti-inflammatory activities	833:882	their antioxidant and anti-inflammatory activities	833:882	hirtum and Thymus capitatus essential oils, selected on the basis of their antioxidant and anti-inflammatory activities.
29966744	4	23	theme	capitatus	782:790	arg1	oils					802:805	capitatus essential oils	782:805	capitatus essential oils	782:805	hirtum and Thymus capitatus essential oils, selected on the basis of their antioxidant and anti-inflammatory activities.
29966744	9	24	theme	In	1569:1570	arg1	viability					1594:1602	In vitro biological cell viability	1569:1602	In vitro biological cell viability	1569:1602	In vitro biological cell viability and anti-inflammatory activities were evaluated in Raw 264.7 cells (macrophage cell line), while in vitro antioxidant activity was checked by DPPH assay.
29966744	0	25	theme	essential	14:22	arg1	oils					24:27	Mediterranean essential oils	0:27	Mediterranean essential oils as precious matrix components and active ingredients of lipid nanoparticles	0:103	Mediterranean essential oils as precious matrix components and active ingredients of lipid nanoparticles.
29966744	10	26	theme	dose-dependent	1912:1925	arg1	activity					1945:1952	a dose-dependent anti-inflammatory activity	1910:1952	a dose-dependent anti-inflammatory activity	1910:1952	Lavandula and Rosmarinus NLC were shown to be the most biocompatible formulations up to a concentration of 0.1% (v/v), whereas they were able to induce a dose-dependent anti-inflammatory activity in the order Lavandula > Rosmarinus ≥ Origanum.
29966744	4	27	theme	activities	873:882	arg1	basis					824:828	the basis	820:828	the basis of their antioxidant and anti-inflammatory activities	820:882	hirtum and Thymus capitatus essential oils, selected on the basis of their antioxidant and anti-inflammatory activities.
29966744	8	28	dep	method	1489:1494	arg1	the					1474:1476	the	1474:1476	the	1474:1476	Accelerated stability studies have also been carried out to estimate the effect of the production method and surfactant composition on the long-term stability of EOs-loaded NLC.
29966744	7	29	theme	essential	1330:1338	arg1	Origanum					1318:1325	Origanum	1318:1325	Origanum	1318:1325	Best NLC formulations were obtained with Kolliphor/Labrafil as surfactants, and using Rosmarinus, Lavandula and Origanum as essential oils (PDI between 0.126 and 0.141, Zave < 200 nm).
29966744	7	29	theme	essential	1330:1338	arg1	oils					1340:1343	essential oils	1330:1343	essential oils (PDI between 0.126 and 0.141, Zave < 200 nm)	1330:1388	Best NLC formulations were obtained with Kolliphor/Labrafil as surfactants, and using Rosmarinus, Lavandula and Origanum as essential oils (PDI between 0.126 and 0.141, Zave < 200 nm).
29966744	7	29	theme	essential	1330:1338	arg1	Lavandula					1304:1312	Lavandula	1304:1312	Lavandula	1304:1312	Best NLC formulations were obtained with Kolliphor/Labrafil as surfactants, and using Rosmarinus, Lavandula and Origanum as essential oils (PDI between 0.126 and 0.141, Zave < 200 nm).
29966744	0	30	theme	Mediterranean	0:12	arg1	oils					24:27	Mediterranean essential oils	0:27	Mediterranean essential oils as precious matrix components and active ingredients of lipid nanoparticles	0:103	Mediterranean essential oils as precious matrix components and active ingredients of lipid nanoparticles.
29966744	8	31	theme	NLC	1564:1566	arg1	stability					1540:1548	the long-term stability	1526:1548	the long-term stability of EOs-loaded NLC	1526:1566	Accelerated stability studies have also been carried out to estimate the effect of the production method and surfactant composition on the long-term stability of EOs-loaded NLC.
29966744	4	32	theme	anti-inflammatory	855:871	arg1	activities					873:882	their antioxidant and anti-inflammatory activities	833:882	their antioxidant and anti-inflammatory activities	833:882	hirtum and Thymus capitatus essential oils, selected on the basis of their antioxidant and anti-inflammatory activities.
29966744	6	33	theme	zeta	1152:1155	arg1	potential					1157:1165	zeta potential	1152:1165	zeta potential	1152:1165	Particles have been further characterized for their mean particle size, polydispersity, zeta potential, morphology and chemical interactions.
29966744	8	34	theme	method	1489:1494	arg1	effect					1464:1469	the effect	1460:1469	the effect of the production method and surfactant composition on the long-term stability of EOs-loaded NLC	1460:1566	Accelerated stability studies have also been carried out to estimate the effect of the production method and surfactant composition on the long-term stability of EOs-loaded NLC.
29966744	8	35	theme	EOs-loaded	1553:1562	arg1	NLC					1564:1566	EOs-loaded NLC	1553:1566	EOs-loaded NLC	1553:1566	Accelerated stability studies have also been carried out to estimate the effect of the production method and surfactant composition on the long-term stability of EOs-loaded NLC.
29966744	9	36	theme	in	1701:1702	arg1	activity					1722:1729	in vitro antioxidant activity	1701:1729	in vitro antioxidant activity	1701:1729	In vitro biological cell viability and anti-inflammatory activities were evaluated in Raw 264.7 cells (macrophage cell line), while in vitro antioxidant activity was checked by DPPH assay.
29966744	1	37	theme	Essential	106:114	arg1	ingredients					170:180	valuable active pharmaceutical ingredients	139:180	valuable active pharmaceutical ingredients attributed to a set of biological properties, which include antibacterial, antifungal, antiviral, antioxidant, anticancer, immune-modulatory, analgesic and anti-inflammatory activities	139:365	Essential oils are recognized as valuable active pharmaceutical ingredients attributed to a set of biological properties, which include antibacterial, antifungal, antiviral, antioxidant, anticancer, immune-modulatory, analgesic and anti-inflammatory activities.
29966744	1	37	theme	Essential	106:114	arg1	oils					116:119	Essential oils	106:119	Essential oils	106:119	Essential oils are recognized as valuable active pharmaceutical ingredients attributed to a set of biological properties, which include antibacterial, antifungal, antiviral, antioxidant, anticancer, immune-modulatory, analgesic and anti-inflammatory activities.
29966744	8	38	theme	stability	1403:1411	arg1	studies					1413:1419	Accelerated stability studies	1391:1419	Accelerated stability studies	1391:1419	Accelerated stability studies have also been carried out to estimate the effect of the production method and surfactant composition on the long-term stability of EOs-loaded NLC.
29966744	2	39	theme	low	456:458	arg1	stability					476:484	low physicochemical stability	456:484	low physicochemical stability	456:484	Their use in pharmaceutics is however compromised by their limited water solubility and low physicochemical stability (i.e. volatility, oxidation).
29966744	2	40	theme	physicochemical	460:474	arg1	stability					476:484	low physicochemical stability	456:484	low physicochemical stability	456:484	Their use in pharmaceutics is however compromised by their limited water solubility and low physicochemical stability (i.e. volatility, oxidation).
29966744	3	41	theme	vulgare	749:755	arg1	carriers					597:604	nanostructured lipid carriers	576:604	nanostructured lipid carriers (NLC)	576:610	In order to overcome these limitations, we aimed to develop nanostructured lipid carriers (NLC) as delivery systems for Mediterranean essential oils, in particular Rosmarinus officinalis L., Lavandula x intermedia "Sumian", Origanum vulgare subsp.
29966744	3	41	theme	vulgare	749:755	arg1	subsp					757:761	Origanum vulgare subsp	740:761	Origanum vulgare subsp	740:761	In order to overcome these limitations, we aimed to develop nanostructured lipid carriers (NLC) as delivery systems for Mediterranean essential oils, in particular Rosmarinus officinalis L., Lavandula x intermedia "Sumian", Origanum vulgare subsp.
29966744	3	41	theme	vulgare	749:755	arg1	systems					624:630	delivery systems	615:630	delivery systems for Mediterranean essential oils	615:663	In order to overcome these limitations, we aimed to develop nanostructured lipid carriers (NLC) as delivery systems for Mediterranean essential oils, in particular Rosmarinus officinalis L., Lavandula x intermedia "Sumian", Origanum vulgare subsp.
29966744	8	42	theme	Accelerated	1391:1401	arg1	studies					1413:1419	Accelerated stability studies	1391:1419	Accelerated stability studies	1391:1419	Accelerated stability studies have also been carried out to estimate the effect of the production method and surfactant composition on the long-term stability of EOs-loaded NLC.
29966744	3	43	theme	lipid	591:595	arg1	NLC					607:609	NLC	607:609	NLC	607:609	In order to overcome these limitations, we aimed to develop nanostructured lipid carriers (NLC) as delivery systems for Mediterranean essential oils, in particular Rosmarinus officinalis L., Lavandula x intermedia "Sumian", Origanum vulgare subsp.
29966744	3	43	theme	lipid	591:595	arg1	carriers					597:604	nanostructured lipid carriers	576:604	nanostructured lipid carriers (NLC)	576:610	In order to overcome these limitations, we aimed to develop nanostructured lipid carriers (NLC) as delivery systems for Mediterranean essential oils, in particular Rosmarinus officinalis L., Lavandula x intermedia "Sumian", Origanum vulgare subsp.
29966744	3	43	theme	lipid	591:595	arg1	subsp					757:761	Origanum vulgare subsp	740:761	Origanum vulgare subsp	740:761	In order to overcome these limitations, we aimed to develop nanostructured lipid carriers (NLC) as delivery systems for Mediterranean essential oils, in particular Rosmarinus officinalis L., Lavandula x intermedia "Sumian", Origanum vulgare subsp.
29966744	3	43	theme	lipid	591:595	arg1	systems					624:630	delivery systems	615:630	delivery systems for Mediterranean essential oils	615:663	In order to overcome these limitations, we aimed to develop nanostructured lipid carriers (NLC) as delivery systems for Mediterranean essential oils, in particular Rosmarinus officinalis L., Lavandula x intermedia "Sumian", Origanum vulgare subsp.
29966744	1	44	theme	properties	216:225	arg1	set					198:200	a set	196:200	a set	196:200	Essential oils are recognized as valuable active pharmaceutical ingredients attributed to a set of biological properties, which include antibacterial, antifungal, antiviral, antioxidant, anticancer, immune-modulatory, analgesic and anti-inflammatory activities.
29966744	1	44	theme	properties	216:225	arg1	properties					216:225	biological properties	205:225	biological properties	205:225	Essential oils are recognized as valuable active pharmaceutical ingredients attributed to a set of biological properties, which include antibacterial, antifungal, antiviral, antioxidant, anticancer, immune-modulatory, analgesic and anti-inflammatory activities.
29966744	0	45	theme	matrix	41:46	arg1	components					48:57	precious matrix components	32:57	precious matrix components	32:57	Mediterranean essential oils as precious matrix components and active ingredients of lipid nanoparticles.
29966744	9	46	dep	In	1569:1570	arg1	vitro					1572:1576	vitro	1572:1576	vitro	1572:1576	In vitro biological cell viability and anti-inflammatory activities were evaluated in Raw 264.7 cells (macrophage cell line), while in vitro antioxidant activity was checked by DPPH assay.
29966744	9	47	theme	antioxidant	1710:1720	arg1	activity					1722:1729	in vitro antioxidant activity	1701:1729	in vitro antioxidant activity	1701:1729	In vitro biological cell viability and anti-inflammatory activities were evaluated in Raw 264.7 cells (macrophage cell line), while in vitro antioxidant activity was checked by DPPH assay.
29966744	3	48	theme	particular	669:678	arg1	officinalis					691:701	particular Rosmarinus officinalis L., Lavandula x intermedia "Sumian"	669:737	officinalis	691:701	In order to overcome these limitations, we aimed to develop nanostructured lipid carriers (NLC) as delivery systems for Mediterranean essential oils, in particular Rosmarinus officinalis L., Lavandula x intermedia "Sumian", Origanum vulgare subsp.
29966744	2	49	dep	solubility	441:450	arg1	oxidation					504:512	oxidation	504:512	oxidation	504:512	Their use in pharmaceutics is however compromised by their limited water solubility and low physicochemical stability (i.e. volatility, oxidation).
29966744	2	49	dep	solubility	441:450	arg1	volatility					492:501	volatility	492:501	volatility	492:501	Their use in pharmaceutics is however compromised by their limited water solubility and low physicochemical stability (i.e. volatility, oxidation).
29966744	0	50	theme	precious	32:39	arg1	components					48:57	precious matrix components	32:57	precious matrix components	32:57	Mediterranean essential oils as precious matrix components and active ingredients of lipid nanoparticles.
29966744	3	51	theme	delivery	615:622	arg1	carriers					597:604	nanostructured lipid carriers	576:604	nanostructured lipid carriers (NLC)	576:610	In order to overcome these limitations, we aimed to develop nanostructured lipid carriers (NLC) as delivery systems for Mediterranean essential oils, in particular Rosmarinus officinalis L., Lavandula x intermedia "Sumian", Origanum vulgare subsp.
29966744	3	51	theme	delivery	615:622	arg1	subsp					757:761	Origanum vulgare subsp	740:761	Origanum vulgare subsp	740:761	In order to overcome these limitations, we aimed to develop nanostructured lipid carriers (NLC) as delivery systems for Mediterranean essential oils, in particular Rosmarinus officinalis L., Lavandula x intermedia "Sumian", Origanum vulgare subsp.
29966744	3	51	theme	delivery	615:622	arg1	systems					624:630	delivery systems	615:630	delivery systems for Mediterranean essential oils	615:663	In order to overcome these limitations, we aimed to develop nanostructured lipid carriers (NLC) as delivery systems for Mediterranean essential oils, in particular Rosmarinus officinalis L., Lavandula x intermedia "Sumian", Origanum vulgare subsp.
29966744	2	52	theme	water	435:439	arg1	solubility					441:450	their limited water solubility	421:450	their limited water solubility	421:450	Their use in pharmaceutics is however compromised by their limited water solubility and low physicochemical stability (i.e. volatility, oxidation).
29966744	9	53	theme	anti-inflammatory	1608:1624	arg1	activities					1626:1635	anti-inflammatory activities	1608:1635	anti-inflammatory activities	1608:1635	In vitro biological cell viability and anti-inflammatory activities were evaluated in Raw 264.7 cells (macrophage cell line), while in vitro antioxidant activity was checked by DPPH assay.
29966744	1	54	dep	antibacterial	242:254	arg1	analgesic					324:332	analgesic	324:332	analgesic	324:332	Essential oils are recognized as valuable active pharmaceutical ingredients attributed to a set of biological properties, which include antibacterial, antifungal, antiviral, antioxidant, anticancer, immune-modulatory, analgesic and anti-inflammatory activities.
29966744	1	54	dep	antibacterial	242:254	arg1	anti-inflammatory					338:354	anti-inflammatory	338:354	anti-inflammatory	338:354	Essential oils are recognized as valuable active pharmaceutical ingredients attributed to a set of biological properties, which include antibacterial, antifungal, antiviral, antioxidant, anticancer, immune-modulatory, analgesic and anti-inflammatory activities.
29966744	1	54	dep	antibacterial	242:254	arg1	antifungal					257:266	antifungal	257:266	antifungal	257:266	Essential oils are recognized as valuable active pharmaceutical ingredients attributed to a set of biological properties, which include antibacterial, antifungal, antiviral, antioxidant, anticancer, immune-modulatory, analgesic and anti-inflammatory activities.
29966744	1	54	dep	antibacterial	242:254	arg1	anticancer					293:302	anticancer	293:302	anticancer	293:302	Essential oils are recognized as valuable active pharmaceutical ingredients attributed to a set of biological properties, which include antibacterial, antifungal, antiviral, antioxidant, anticancer, immune-modulatory, analgesic and anti-inflammatory activities.
29966744	1	54	dep	antibacterial	242:254	arg1	immune-modulatory					305:321	immune-modulatory	305:321	immune-modulatory	305:321	Essential oils are recognized as valuable active pharmaceutical ingredients attributed to a set of biological properties, which include antibacterial, antifungal, antiviral, antioxidant, anticancer, immune-modulatory, analgesic and anti-inflammatory activities.
29966744	1	54	dep	antibacterial	242:254	arg1	antiviral					269:277	antiviral	269:277	antiviral	269:277	Essential oils are recognized as valuable active pharmaceutical ingredients attributed to a set of biological properties, which include antibacterial, antifungal, antiviral, antioxidant, anticancer, immune-modulatory, analgesic and anti-inflammatory activities.
29966744	1	54	dep	antibacterial	242:254	arg1	antioxidant					280:290	antioxidant	280:290	antioxidant	280:290	Essential oils are recognized as valuable active pharmaceutical ingredients attributed to a set of biological properties, which include antibacterial, antifungal, antiviral, antioxidant, anticancer, immune-modulatory, analgesic and anti-inflammatory activities.
29966744	10	55	dep	shown	1792:1796	arg1	whereas					1877:1883	whereas	1877:1883	whereas	1877:1883	Lavandula and Rosmarinus NLC were shown to be the most biocompatible formulations up to a concentration of 0.1% (v/v), whereas they were able to induce a dose-dependent anti-inflammatory activity in the order Lavandula > Rosmarinus ≥ Origanum.
29966744	4	56	dep	hirtum	764:769	arg1	oils					802:805	capitatus essential oils	782:805	capitatus essential oils	782:805	hirtum and Thymus capitatus essential oils, selected on the basis of their antioxidant and anti-inflammatory activities.
29966744	5	57	theme	phase	949:953	arg1	PIT					978:980	PIT	978:980	PIT	978:980	NLC composed of Softisan (as solid lipid) have been produced by phase inversion temperature (PIT) and high-pressure homogenization (HPH), using two different emulsifiers systems.
29966744	5	57	theme	phase	949:953	arg1	temperature					965:975	phase inversion temperature	949:975	phase inversion temperature (PIT)	949:981	NLC composed of Softisan (as solid lipid) have been produced by phase inversion temperature (PIT) and high-pressure homogenization (HPH), using two different emulsifiers systems.
29966744	10	58	theme	order	1961:1965	arg1	Lavandula > Rosmarinus ≥ Origanum					1967:1999	the order Lavandula > Rosmarinus ≥ Origanum	1957:1999	the order Lavandula > Rosmarinus ≥ Origanum	1957:1999	Lavandula and Rosmarinus NLC were shown to be the most biocompatible formulations up to a concentration of 0.1% (v/v), whereas they were able to induce a dose-dependent anti-inflammatory activity in the order Lavandula > Rosmarinus ≥ Origanum.
29966744	8	59	theme	surfactant	1500:1509	arg1	composition					1511:1521	surfactant composition	1500:1521	surfactant composition	1500:1521	Accelerated stability studies have also been carried out to estimate the effect of the production method and surfactant composition on the long-term stability of EOs-loaded NLC.
29966744	9	60	theme	biological	1578:1587	arg1	viability					1594:1602	In vitro biological cell viability	1569:1602	In vitro biological cell viability	1569:1602	In vitro biological cell viability and anti-inflammatory activities were evaluated in Raw 264.7 cells (macrophage cell line), while in vitro antioxidant activity was checked by DPPH assay.
29966744	10	61	theme	Rosmarinus	1772:1781	arg1	Lavandula					1758:1766	Lavandula	1758:1766	Lavandula	1758:1766	Lavandula and Rosmarinus NLC were shown to be the most biocompatible formulations up to a concentration of 0.1% (v/v), whereas they were able to induce a dose-dependent anti-inflammatory activity in the order Lavandula > Rosmarinus ≥ Origanum.
29966744	10	61	theme	Rosmarinus	1772:1781	arg1	NLC					1783:1785	Rosmarinus NLC	1772:1785	Rosmarinus NLC	1772:1785	Lavandula and Rosmarinus NLC were shown to be the most biocompatible formulations up to a concentration of 0.1% (v/v), whereas they were able to induce a dose-dependent anti-inflammatory activity in the order Lavandula > Rosmarinus ≥ Origanum.
29966744	10	61	theme	Rosmarinus	1772:1781	arg1	formulations					1827:1838	the most biocompatible formulations	1804:1838	the most biocompatible formulations up to a concentration of 0.1% (v/v)	1804:1874	Lavandula and Rosmarinus NLC were shown to be the most biocompatible formulations up to a concentration of 0.1% (v/v), whereas they were able to induce a dose-dependent anti-inflammatory activity in the order Lavandula > Rosmarinus ≥ Origanum.
29966744	10	62	theme	biocompatible	1813:1825	arg1	Lavandula					1758:1766	Lavandula	1758:1766	Lavandula	1758:1766	Lavandula and Rosmarinus NLC were shown to be the most biocompatible formulations up to a concentration of 0.1% (v/v), whereas they were able to induce a dose-dependent anti-inflammatory activity in the order Lavandula > Rosmarinus ≥ Origanum.
29966744	10	62	theme	biocompatible	1813:1825	arg1	formulations					1827:1838	the most biocompatible formulations	1804:1838	the most biocompatible formulations up to a concentration of 0.1% (v/v)	1804:1874	Lavandula and Rosmarinus NLC were shown to be the most biocompatible formulations up to a concentration of 0.1% (v/v), whereas they were able to induce a dose-dependent anti-inflammatory activity in the order Lavandula > Rosmarinus ≥ Origanum.
29966744	10	62	theme	biocompatible	1813:1825	arg1	NLC					1783:1785	Rosmarinus NLC	1772:1785	Rosmarinus NLC	1772:1785	Lavandula and Rosmarinus NLC were shown to be the most biocompatible formulations up to a concentration of 0.1% (v/v), whereas they were able to induce a dose-dependent anti-inflammatory activity in the order Lavandula > Rosmarinus ≥ Origanum.
29966744	5	63	theme	different	1033:1041	arg1	systems					1055:1061	two different emulsifiers systems	1029:1061	two different emulsifiers systems	1029:1061	NLC composed of Softisan (as solid lipid) have been produced by phase inversion temperature (PIT) and high-pressure homogenization (HPH), using two different emulsifiers systems.
29966744	3	64	theme	Mediterranean	636:648	arg1	oils					660:663	Mediterranean essential oils	636:663	Mediterranean essential oils	636:663	In order to overcome these limitations, we aimed to develop nanostructured lipid carriers (NLC) as delivery systems for Mediterranean essential oils, in particular Rosmarinus officinalis L., Lavandula x intermedia "Sumian", Origanum vulgare subsp.
29966744	7	65	dep	oils	1340:1343	arg1	Zave < 200 nm					1375:1387	Zave < 200 nm	1375:1387	Zave < 200 nm	1375:1387	Best NLC formulations were obtained with Kolliphor/Labrafil as surfactants, and using Rosmarinus, Lavandula and Origanum as essential oils (PDI between 0.126 and 0.141, Zave < 200 nm).
29966744	7	65	dep	oils	1340:1343	arg1	PDI					1346:1348	PDI	1346:1348	PDI between 0.126 and 0.141	1346:1372	Best NLC formulations were obtained with Kolliphor/Labrafil as surfactants, and using Rosmarinus, Lavandula and Origanum as essential oils (PDI between 0.126 and 0.141, Zave < 200 nm).
29966744	6	66	theme	particle	1121:1128	arg1	size					1130:1133	their mean particle size	1110:1133	their mean particle size	1110:1133	Particles have been further characterized for their mean particle size, polydispersity, zeta potential, morphology and chemical interactions.
29966744	3	67	theme	nanostructured	576:589	arg1	NLC					607:609	NLC	607:609	NLC	607:609	In order to overcome these limitations, we aimed to develop nanostructured lipid carriers (NLC) as delivery systems for Mediterranean essential oils, in particular Rosmarinus officinalis L., Lavandula x intermedia "Sumian", Origanum vulgare subsp.
29966744	3	67	theme	nanostructured	576:589	arg1	carriers					597:604	nanostructured lipid carriers	576:604	nanostructured lipid carriers (NLC)	576:610	In order to overcome these limitations, we aimed to develop nanostructured lipid carriers (NLC) as delivery systems for Mediterranean essential oils, in particular Rosmarinus officinalis L., Lavandula x intermedia "Sumian", Origanum vulgare subsp.
29966744	3	67	theme	nanostructured	576:589	arg1	subsp					757:761	Origanum vulgare subsp	740:761	Origanum vulgare subsp	740:761	In order to overcome these limitations, we aimed to develop nanostructured lipid carriers (NLC) as delivery systems for Mediterranean essential oils, in particular Rosmarinus officinalis L., Lavandula x intermedia "Sumian", Origanum vulgare subsp.
29966744	3	67	theme	nanostructured	576:589	arg1	systems					624:630	delivery systems	615:630	delivery systems for Mediterranean essential oils	615:663	In order to overcome these limitations, we aimed to develop nanostructured lipid carriers (NLC) as delivery systems for Mediterranean essential oils, in particular Rosmarinus officinalis L., Lavandula x intermedia "Sumian", Origanum vulgare subsp.
29966744	9	68	theme	DPPH	1746:1749	arg1	assay					1751:1755	DPPH assay	1746:1755	DPPH assay	1746:1755	In vitro biological cell viability and anti-inflammatory activities were evaluated in Raw 264.7 cells (macrophage cell line), while in vitro antioxidant activity was checked by DPPH assay.
29966744	3	69	theme	Rosmarinus	680:689	arg1	officinalis					691:701	particular Rosmarinus officinalis L., Lavandula x intermedia "Sumian"	669:737	officinalis	691:701	In order to overcome these limitations, we aimed to develop nanostructured lipid carriers (NLC) as delivery systems for Mediterranean essential oils, in particular Rosmarinus officinalis L., Lavandula x intermedia "Sumian", Origanum vulgare subsp.
29966744	3	70	theme	essential	650:658	arg1	oils					660:663	Mediterranean essential oils	636:663	Mediterranean essential oils	636:663	In order to overcome these limitations, we aimed to develop nanostructured lipid carriers (NLC) as delivery systems for Mediterranean essential oils, in particular Rosmarinus officinalis L., Lavandula x intermedia "Sumian", Origanum vulgare subsp.
29966744	1	71	theme	valuable	139:146	arg1	ingredients					170:180	valuable active pharmaceutical ingredients	139:180	valuable active pharmaceutical ingredients attributed to a set of biological properties, which include antibacterial, antifungal, antiviral, antioxidant, anticancer, immune-modulatory, analgesic and anti-inflammatory activities	139:365	Essential oils are recognized as valuable active pharmaceutical ingredients attributed to a set of biological properties, which include antibacterial, antifungal, antiviral, antioxidant, anticancer, immune-modulatory, analgesic and anti-inflammatory activities.
29966744	1	71	theme	valuable	139:146	arg1	oils					116:119	Essential oils	106:119	Essential oils	106:119	Essential oils are recognized as valuable active pharmaceutical ingredients attributed to a set of biological properties, which include antibacterial, antifungal, antiviral, antioxidant, anticancer, immune-modulatory, analgesic and anti-inflammatory activities.
29966744	5	72	theme	emulsifiers	1043:1053	arg1	systems					1055:1061	two different emulsifiers systems	1029:1061	two different emulsifiers systems	1029:1061	NLC composed of Softisan (as solid lipid) have been produced by phase inversion temperature (PIT) and high-pressure homogenization (HPH), using two different emulsifiers systems.
29966744	1	73	theme	antibacterial	242:254	arg1	activities					356:365	antibacterial, antifungal, antiviral, antioxidant, anticancer, immune-modulatory, analgesic and anti-inflammatory activities	242:365	antibacterial, antifungal, antiviral, antioxidant, anticancer, immune-modulatory, analgesic and anti-inflammatory activities	242:365	Essential oils are recognized as valuable active pharmaceutical ingredients attributed to a set of biological properties, which include antibacterial, antifungal, antiviral, antioxidant, anticancer, immune-modulatory, analgesic and anti-inflammatory activities.
29966744	8	74	from	effect	1464:1469	arg1	stability					1540:1548	the long-term stability	1526:1548	the long-term stability of EOs-loaded NLC	1526:1566	Accelerated stability studies have also been carried out to estimate the effect of the production method and surfactant composition on the long-term stability of EOs-loaded NLC.
29966744	8	75	theme	production	1478:1487	arg1	method					1489:1494	production method	1478:1494	production method	1478:1494	Accelerated stability studies have also been carried out to estimate the effect of the production method and surfactant composition on the long-term stability of EOs-loaded NLC.
29966744	9	76	theme	Raw	1655:1657	arg1	cells					1665:1669	Raw 264.7 cells	1655:1669	Raw 264.7 cells (macrophage cell line)	1655:1692	In vitro biological cell viability and anti-inflammatory activities were evaluated in Raw 264.7 cells (macrophage cell line), while in vitro antioxidant activity was checked by DPPH assay.
29966744	9	76	theme	Raw	1655:1657	arg1	line					1688:1691	macrophage cell line	1672:1691	macrophage cell line	1672:1691	In vitro biological cell viability and anti-inflammatory activities were evaluated in Raw 264.7 cells (macrophage cell line), while in vitro antioxidant activity was checked by DPPH assay.
29966744	1	77	theme	biological	205:214	arg1	properties					216:225	biological properties	205:225	biological properties	205:225	Essential oils are recognized as valuable active pharmaceutical ingredients attributed to a set of biological properties, which include antibacterial, antifungal, antiviral, antioxidant, anticancer, immune-modulatory, analgesic and anti-inflammatory activities.
29966744	1	78	theme	active	148:153	arg1	ingredients					170:180	valuable active pharmaceutical ingredients	139:180	valuable active pharmaceutical ingredients attributed to a set of biological properties, which include antibacterial, antifungal, antiviral, antioxidant, anticancer, immune-modulatory, analgesic and anti-inflammatory activities	139:365	Essential oils are recognized as valuable active pharmaceutical ingredients attributed to a set of biological properties, which include antibacterial, antifungal, antiviral, antioxidant, anticancer, immune-modulatory, analgesic and anti-inflammatory activities.
29966744	1	78	theme	active	148:153	arg1	oils					116:119	Essential oils	106:119	Essential oils	106:119	Essential oils are recognized as valuable active pharmaceutical ingredients attributed to a set of biological properties, which include antibacterial, antifungal, antiviral, antioxidant, anticancer, immune-modulatory, analgesic and anti-inflammatory activities.
29966744	10	79	theme	%	1868:1868	arg1	concentration					1848:1860	a concentration	1846:1860	a concentration of 0.1% (v/v)	1846:1874	Lavandula and Rosmarinus NLC were shown to be the most biocompatible formulations up to a concentration of 0.1% (v/v), whereas they were able to induce a dose-dependent anti-inflammatory activity in the order Lavandula > Rosmarinus ≥ Origanum.
29966744	0	80	theme	active	63:68	arg1	ingredients					70:80	active ingredients	63:80	active ingredients of lipid nanoparticles	63:103	Mediterranean essential oils as precious matrix components and active ingredients of lipid nanoparticles.
29966744	3	81	theme	intermedia	719:728	arg1	"					737:737	intermedia "Sumian"	719:737	intermedia "Sumian"	719:737	In order to overcome these limitations, we aimed to develop nanostructured lipid carriers (NLC) as delivery systems for Mediterranean essential oils, in particular Rosmarinus officinalis L., Lavandula x intermedia "Sumian", Origanum vulgare subsp.
29966744	9	82	theme	cell	1589:1592	arg1	viability					1594:1602	In vitro biological cell viability	1569:1602	In vitro biological cell viability	1569:1602	In vitro biological cell viability and anti-inflammatory activities were evaluated in Raw 264.7 cells (macrophage cell line), while in vitro antioxidant activity was checked by DPPH assay.
29966744	1	83	theme	pharmaceutical	155:168	arg1	ingredients					170:180	valuable active pharmaceutical ingredients	139:180	valuable active pharmaceutical ingredients attributed to a set of biological properties, which include antibacterial, antifungal, antiviral, antioxidant, anticancer, immune-modulatory, analgesic and anti-inflammatory activities	139:365	Essential oils are recognized as valuable active pharmaceutical ingredients attributed to a set of biological properties, which include antibacterial, antifungal, antiviral, antioxidant, anticancer, immune-modulatory, analgesic and anti-inflammatory activities.
29966744	1	83	theme	pharmaceutical	155:168	arg1	oils					116:119	Essential oils	106:119	Essential oils	106:119	Essential oils are recognized as valuable active pharmaceutical ingredients attributed to a set of biological properties, which include antibacterial, antifungal, antiviral, antioxidant, anticancer, immune-modulatory, analgesic and anti-inflammatory activities.
29966744	7	84	theme	NLC	1211:1213	arg1	surfactants					1269:1279	surfactants	1269:1279	surfactants	1269:1279	Best NLC formulations were obtained with Kolliphor/Labrafil as surfactants, and using Rosmarinus, Lavandula and Origanum as essential oils (PDI between 0.126 and 0.141, Zave < 200 nm).
29966744	7	84	theme	NLC	1211:1213	arg1	Origanum					1318:1325	Origanum	1318:1325	Origanum	1318:1325	Best NLC formulations were obtained with Kolliphor/Labrafil as surfactants, and using Rosmarinus, Lavandula and Origanum as essential oils (PDI between 0.126 and 0.141, Zave < 200 nm).
29966744	7	84	theme	NLC	1211:1213	arg1	Lavandula					1304:1312	Lavandula	1304:1312	Lavandula	1304:1312	Best NLC formulations were obtained with Kolliphor/Labrafil as surfactants, and using Rosmarinus, Lavandula and Origanum as essential oils (PDI between 0.126 and 0.141, Zave < 200 nm).
29966744	7	84	theme	NLC	1211:1213	arg1	formulations					1215:1226	Best NLC formulations	1206:1226	Best NLC formulations	1206:1226	Best NLC formulations were obtained with Kolliphor/Labrafil as surfactants, and using Rosmarinus, Lavandula and Origanum as essential oils (PDI between 0.126 and 0.141, Zave < 200 nm).
30592165	2	0	theme	peer-to-peer	453:464	arg1	milk					466:469	peer-to-peer milk	453:469	peer-to-peer milk sharing	453:477	There is a paucity of research regarding the nutrient and bioactive profiles of expressed human milk exchanged through commerce-free scenarios, including peer-to-peer milk sharing.
30592165	0	1	from	study	8:12	arg1	bacteria					56:63	bacteria	56:63	bacteria	56:63	A pilot study on nutrients, antimicrobial proteins, and bacteria in commerce-free models for exchanging expressed human milk in the USA.
30592165	0	1	from	study	8:12	arg1	models					82:87	commerce-free models	68:87	commerce-free models for exchanging	68:102	A pilot study on nutrients, antimicrobial proteins, and bacteria in commerce-free models for exchanging expressed human milk in the USA.
30592165	0	1	from	study	8:12	arg1	nutrients					17:25	nutrients	17:25	nutrients	17:25	A pilot study on nutrients, antimicrobial proteins, and bacteria in commerce-free models for exchanging expressed human milk in the USA.
30592165	0	1	from	study	8:12	arg1	proteins					42:49	antimicrobial proteins	28:49	antimicrobial proteins	28:49	A pilot study on nutrients, antimicrobial proteins, and bacteria in commerce-free models for exchanging expressed human milk in the USA.
30592165	8	2	dep	1.5 g/dL	1591:1598	arg1	1.6					1606:1608	1.6	1606:1608	1.6	1606:1608	Mean protein values (95% confidence interval) were 1.5 g/dL (1.4, 1.6) for BANKED, 1.4 g/dL (1.3, 1.5) for MOM, 1.6 g/dL (1.5, 1.7) for SCREENED, and 1.5 g/dL (1.4, 1.6) for SHARED, which was not significantly different (p = .081).
30592165	1	3	theme	sharing	290:296	arg1	milk					285:288	peer-to-peer milk sharing	272:296	peer-to-peer milk sharing	272:296	Expressed human milk can be donated or sold through a variety of channels, including human milk banks, corporations or individuals, or peer-to-peer milk sharing.
30592165	1	4	theme	Expressed	137:145	arg1	milk					153:156	Expressed human milk	137:156	Expressed human milk	137:156	Expressed human milk can be donated or sold through a variety of channels, including human milk banks, corporations or individuals, or peer-to-peer milk sharing.
30592165	5	5	theme	immunoglobulin	1167:1180	arg1	IgA					1185:1187	IgA	1185:1187	IgA	1185:1187	Analyses were conducted for total protein, lactose, percent fat and water, lysozyme activity, immunoglobulin A (IgA) activity, total aerobic bacteria, coliform, and Staphylococcus aureus.
30592165	5	5	theme	immunoglobulin	1167:1180	arg1	A					1182:1182	immunoglobulin A	1167:1182	immunoglobulin A (IgA) activity	1167:1197	Analyses were conducted for total protein, lactose, percent fat and water, lysozyme activity, immunoglobulin A (IgA) activity, total aerobic bacteria, coliform, and Staphylococcus aureus.
30592165	9	6	dep	risks	1827:1831	arg1	the					1823:1825	the	1823:1825	the	1823:1825	This research contributes to growing literature on the risks and benefits of uncompensated, peer-to-peer milk sharing.
30592165	4	7	theme	own	778:780	arg1	MOM					790:792	MOM	790:792	MOM	790:792	Expressed human milk samples were collected from the following commerce-free scenarios: milk expressed for a mother's or parent's own infant (MOM; N = 30); unpasteurized milk donated to a non-profit milk bank (BANKED; N = 30); milk expressed for peer-to-peer milk sharing (SHARED; N = 31); and health professional-facilitated milk sharing where donors are serologically screened and milk is dispensed raw (SCREENED; N = 30).
30592165	4	7	theme	own	778:780	arg1	infant					782:787	parent's own infant	769:787	parent's own infant (MOM; N = 30)	769:801	Expressed human milk samples were collected from the following commerce-free scenarios: milk expressed for a mother's or parent's own infant (MOM; N = 30); unpasteurized milk donated to a non-profit milk bank (BANKED; N = 30); milk expressed for peer-to-peer milk sharing (SHARED; N = 31); and health professional-facilitated milk sharing where donors are serologically screened and milk is dispensed raw (SCREENED; N = 30).
30592165	4	7	theme	own	778:780	arg1	scenarios					725:733	the following commerce-free scenarios	697:733	the following commerce-free scenarios: milk expressed for a mother's or parent's own infant (MOM; N = 30)	697:801	Expressed human milk samples were collected from the following commerce-free scenarios: milk expressed for a mother's or parent's own infant (MOM; N = 30); unpasteurized milk donated to a non-profit milk bank (BANKED; N = 30); milk expressed for peer-to-peer milk sharing (SHARED; N = 31); and health professional-facilitated milk sharing where donors are serologically screened and milk is dispensed raw (SCREENED; N = 30).
30592165	4	8	theme	non-profit	836:845	arg1	BANKED					858:863	BANKED	858:863	BANKED	858:863	Expressed human milk samples were collected from the following commerce-free scenarios: milk expressed for a mother's or parent's own infant (MOM; N = 30); unpasteurized milk donated to a non-profit milk bank (BANKED; N = 30); milk expressed for peer-to-peer milk sharing (SHARED; N = 31); and health professional-facilitated milk sharing where donors are serologically screened and milk is dispensed raw (SCREENED; N = 30).
30592165	4	8	theme	non-profit	836:845	arg1	bank					852:855	a non-profit milk bank	834:855	a non-profit milk bank (BANKED; N = 30)	834:872	Expressed human milk samples were collected from the following commerce-free scenarios: milk expressed for a mother's or parent's own infant (MOM; N = 30); unpasteurized milk donated to a non-profit milk bank (BANKED; N = 30); milk expressed for peer-to-peer milk sharing (SHARED; N = 31); and health professional-facilitated milk sharing where donors are serologically screened and milk is dispensed raw (SCREENED; N = 30).
30592165	4	9	theme	Expressed	648:656	arg1	samples					669:675	Expressed human milk samples	648:675	Expressed human milk samples	648:675	Expressed human milk samples were collected from the following commerce-free scenarios: milk expressed for a mother's or parent's own infant (MOM; N = 30); unpasteurized milk donated to a non-profit milk bank (BANKED; N = 30); milk expressed for peer-to-peer milk sharing (SHARED; N = 31); and health professional-facilitated milk sharing where donors are serologically screened and milk is dispensed raw (SCREENED; N = 30).
30592165	1	10	theme	human	222:226	arg1	banks					233:237	human milk banks	222:237	human milk banks	222:237	Expressed human milk can be donated or sold through a variety of channels, including human milk banks, corporations or individuals, or peer-to-peer milk sharing.
30592165	0	11	theme	commerce-free	68:80	arg1	models					82:87	commerce-free models	68:87	commerce-free models for exchanging	68:102	A pilot study on nutrients, antimicrobial proteins, and bacteria in commerce-free models for exchanging expressed human milk in the USA.
30592165	5	12	theme	lysozyme	1148:1155	arg1	activity					1157:1164	lysozyme activity	1148:1164	lysozyme activity	1148:1164	Analyses were conducted for total protein, lactose, percent fat and water, lysozyme activity, immunoglobulin A (IgA) activity, total aerobic bacteria, coliform, and Staphylococcus aureus.
30592165	9	13	dep	uncompensated	1849:1861	arg1	peer-to-peer					1864:1875	peer-to-peer	1864:1875	peer-to-peer	1864:1875	This research contributes to growing literature on the risks and benefits of uncompensated, peer-to-peer milk sharing.
30592165	1	14	theme	milk	228:231	arg1	banks					233:237	human milk banks	222:237	human milk banks	222:237	Expressed human milk can be donated or sold through a variety of channels, including human milk banks, corporations or individuals, or peer-to-peer milk sharing.
30592165	4	15	theme	peer-to-peer	894:905	arg1	milk					907:910	peer-to-peer milk sharing	894:918	peer-to-peer milk sharing	894:918	Expressed human milk samples were collected from the following commerce-free scenarios: milk expressed for a mother's or parent's own infant (MOM; N = 30); unpasteurized milk donated to a non-profit milk bank (BANKED; N = 30); milk expressed for peer-to-peer milk sharing (SHARED; N = 31); and health professional-facilitated milk sharing where donors are serologically screened and milk is dispensed raw (SCREENED; N = 30).
30592165	8	16	theme	protein	1545:1551	arg1	values					1553:1558	Mean protein values	1540:1558	Mean protein values (95% confidence interval)	1540:1584	Mean protein values (95% confidence interval) were 1.5 g/dL (1.4, 1.6) for BANKED, 1.4 g/dL (1.3, 1.5) for MOM, 1.6 g/dL (1.5, 1.7) for SCREENED, and 1.5 g/dL (1.4, 1.6) for SHARED, which was not significantly different (p = .081).
30592165	1	17	theme	human	147:151	arg1	milk					153:156	Expressed human milk	137:156	Expressed human milk	137:156	Expressed human milk can be donated or sold through a variety of channels, including human milk banks, corporations or individuals, or peer-to-peer milk sharing.
30592165	9	18	theme	milk	1877:1880	arg1	benefits					1837:1844	benefits	1837:1844	benefits	1837:1844	This research contributes to growing literature on the risks and benefits of uncompensated, peer-to-peer milk sharing.
30592165	9	18	theme	milk	1877:1880	arg1	risks					1827:1831	risks	1827:1831	risks	1827:1831	This research contributes to growing literature on the risks and benefits of uncompensated, peer-to-peer milk sharing.
30592165	0	19	theme	pilot	2:6	arg1	study					8:12	A pilot study	0:12	A pilot study on nutrients, antimicrobial proteins, and bacteria in commerce-free models for exchanging	0:102	A pilot study on nutrients, antimicrobial proteins, and bacteria in commerce-free models for exchanging expressed human milk in the USA.
30592165	6	20	theme	bacterial	1264:1272	arg1	growth					1274:1279	No bacterial growth	1261:1279	No bacterial growth	1261:1279	No bacterial growth was observed in 52/121 samples, and 15/121 had growth greater than 5.0 log colony-forming units/mL.
30592165	4	21	dep	BANKED	858:863	arg1	N = 30					866:871	N = 30	866:871	N = 30	866:871	Expressed human milk samples were collected from the following commerce-free scenarios: milk expressed for a mother's or parent's own infant (MOM; N = 30); unpasteurized milk donated to a non-profit milk bank (BANKED; N = 30); milk expressed for peer-to-peer milk sharing (SHARED; N = 31); and health professional-facilitated milk sharing where donors are serologically screened and milk is dispensed raw (SCREENED; N = 30).
30592165	3	22	theme	antimicrobial	535:547	arg1	protein					549:555	antimicrobial protein	535:555	antimicrobial protein	535:555	The study objective was to evaluate the macronutrient, antimicrobial protein, and bacteria composition in expressed human milk acquired via commerce-free arrangements.
30592165	8	23	dep	different	1750:1758	arg1	p = .081					1761:1768	p = .081	1761:1768	p = .081	1761:1768	Mean protein values (95% confidence interval) were 1.5 g/dL (1.4, 1.6) for BANKED, 1.4 g/dL (1.3, 1.5) for MOM, 1.6 g/dL (1.5, 1.7) for SCREENED, and 1.5 g/dL (1.4, 1.6) for SHARED, which was not significantly different (p = .081).
30592165	4	24	theme	milk	664:667	arg1	samples					669:675	Expressed human milk samples	648:675	Expressed human milk samples	648:675	Expressed human milk samples were collected from the following commerce-free scenarios: milk expressed for a mother's or parent's own infant (MOM; N = 30); unpasteurized milk donated to a non-profit milk bank (BANKED; N = 30); milk expressed for peer-to-peer milk sharing (SHARED; N = 31); and health professional-facilitated milk sharing where donors are serologically screened and milk is dispensed raw (SCREENED; N = 30).
30592165	2	25	theme	milk	395:398	arg1	profiles					367:374	the nutrient and bioactive profiles	340:374	the nutrient and bioactive profiles of expressed human milk exchanged through commerce-free scenarios, including peer-to-peer milk sharing	340:477	There is a paucity of research regarding the nutrient and bioactive profiles of expressed human milk exchanged through commerce-free scenarios, including peer-to-peer milk sharing.
30592165	9	26	from	literature	1809:1818	arg1	benefits					1837:1844	benefits	1837:1844	benefits	1837:1844	This research contributes to growing literature on the risks and benefits of uncompensated, peer-to-peer milk sharing.
30592165	9	26	from	literature	1809:1818	arg1	risks					1827:1831	risks	1827:1831	risks	1827:1831	This research contributes to growing literature on the risks and benefits of uncompensated, peer-to-peer milk sharing.
30592165	4	27	theme	unpasteurized	804:816	arg1	milk					818:821	unpasteurized milk	804:821	unpasteurized milk	804:821	Expressed human milk samples were collected from the following commerce-free scenarios: milk expressed for a mother's or parent's own infant (MOM; N = 30); unpasteurized milk donated to a non-profit milk bank (BANKED; N = 30); milk expressed for peer-to-peer milk sharing (SHARED; N = 31); and health professional-facilitated milk sharing where donors are serologically screened and milk is dispensed raw (SCREENED; N = 30).
30592165	8	28	dep	values	1553:1558	arg1	%					1563:1563	95%	1561:1563	95% confidence interval	1561:1583	Mean protein values (95% confidence interval) were 1.5 g/dL (1.4, 1.6) for BANKED, 1.4 g/dL (1.3, 1.5) for MOM, 1.6 g/dL (1.5, 1.7) for SCREENED, and 1.5 g/dL (1.4, 1.6) for SHARED, which was not significantly different (p = .081).
30592165	3	29	theme	protein	549:555	arg1	composition					571:581	the macronutrient, antimicrobial protein, and bacteria composition	516:581	the macronutrient, antimicrobial protein, and bacteria composition in expressed human milk acquired via commerce-free arrangements	516:645	The study objective was to evaluate the macronutrient, antimicrobial protein, and bacteria composition in expressed human milk acquired via commerce-free arrangements.
30592165	2	30	theme	research	321:328	arg1	paucity					310:316	a paucity	308:316	a paucity of research regarding the nutrient and bioactive profiles of expressed human milk exchanged through commerce-free scenarios, including peer-to-peer milk sharing	308:477	There is a paucity of research regarding the nutrient and bioactive profiles of expressed human milk exchanged through commerce-free scenarios, including peer-to-peer milk sharing.
30592165	2	31	theme	human	389:393	arg1	milk					395:398	expressed human milk	379:398	expressed human milk exchanged through commerce-free scenarios, including peer-to-peer milk sharing	379:477	There is a paucity of research regarding the nutrient and bioactive profiles of expressed human milk exchanged through commerce-free scenarios, including peer-to-peer milk sharing.
30592165	5	32	theme	total	1101:1105	arg1	protein					1107:1113	total protein	1101:1113	total protein	1101:1113	Analyses were conducted for total protein, lactose, percent fat and water, lysozyme activity, immunoglobulin A (IgA) activity, total aerobic bacteria, coliform, and Staphylococcus aureus.
30592165	0	33	theme	human	114:118	arg1	milk					120:123	human milk	114:123	human milk	114:123	A pilot study on nutrients, antimicrobial proteins, and bacteria in commerce-free models for exchanging expressed human milk in the USA.
30592165	3	34	theme	macronutrient	520:532	arg1	composition					571:581	the macronutrient, antimicrobial protein, and bacteria composition	516:581	the macronutrient, antimicrobial protein, and bacteria composition in expressed human milk acquired via commerce-free arrangements	516:645	The study objective was to evaluate the macronutrient, antimicrobial protein, and bacteria composition in expressed human milk acquired via commerce-free arrangements.
30592165	5	35	theme	total	1200:1204	arg1	bacteria					1214:1221	total aerobic bacteria	1200:1221	total aerobic bacteria	1200:1221	Analyses were conducted for total protein, lactose, percent fat and water, lysozyme activity, immunoglobulin A (IgA) activity, total aerobic bacteria, coliform, and Staphylococcus aureus.
30592165	2	36	theme	expressed	379:387	arg1	milk					395:398	expressed human milk	379:398	expressed human milk exchanged through commerce-free scenarios, including peer-to-peer milk sharing	379:477	There is a paucity of research regarding the nutrient and bioactive profiles of expressed human milk exchanged through commerce-free scenarios, including peer-to-peer milk sharing.
30592165	7	37	theme	sIgA	1481:1484	arg1	activity					1486:1493	sIgA activity	1481:1493	sIgA activity	1481:1493	There was no evidence of differences by groups (p > .05) in lactose, fat, water, lysozyme activity, sIgA activity, aerobic bacteria, coliforms, and S. aureus.
30592165	6	38	dep	log	1352:1354	arg1	5.0					1348:1350	5.0	1348:1350	5.0	1348:1350	No bacterial growth was observed in 52/121 samples, and 15/121 had growth greater than 5.0 log colony-forming units/mL.
30592165	6	38	dep	log	1352:1354	arg1	growth					1328:1333	growth	1328:1333	growth	1328:1333	No bacterial growth was observed in 52/121 samples, and 15/121 had growth greater than 5.0 log colony-forming units/mL.
30592165	3	39	theme	commerce-free	620:632	arg1	arrangements					634:645	commerce-free arrangements	620:645	commerce-free arrangements	620:645	The study objective was to evaluate the macronutrient, antimicrobial protein, and bacteria composition in expressed human milk acquired via commerce-free arrangements.
30592165	8	40	theme	interval	1576:1583	arg1	%					1563:1563	95%	1561:1563	95% confidence interval	1561:1583	Mean protein values (95% confidence interval) were 1.5 g/dL (1.4, 1.6) for BANKED, 1.4 g/dL (1.3, 1.5) for MOM, 1.6 g/dL (1.5, 1.7) for SCREENED, and 1.5 g/dL (1.4, 1.6) for SHARED, which was not significantly different (p = .081).
30592165	5	41	theme	aerobic	1206:1212	arg1	bacteria					1214:1221	total aerobic bacteria	1200:1221	total aerobic bacteria	1200:1221	Analyses were conducted for total protein, lactose, percent fat and water, lysozyme activity, immunoglobulin A (IgA) activity, total aerobic bacteria, coliform, and Staphylococcus aureus.
30592165	8	42	dep	1.5	1662:1664	arg1	1.7					1667:1669	1.7	1667:1669	1.7	1667:1669	Mean protein values (95% confidence interval) were 1.5 g/dL (1.4, 1.6) for BANKED, 1.4 g/dL (1.3, 1.5) for MOM, 1.6 g/dL (1.5, 1.7) for SCREENED, and 1.5 g/dL (1.4, 1.6) for SHARED, which was not significantly different (p = .081).
30592165	4	43	dep	expressed	880:888	arg1	SHARED					921:926	SHARED	921:926	SHARED	921:926	Expressed human milk samples were collected from the following commerce-free scenarios: milk expressed for a mother's or parent's own infant (MOM; N = 30); unpasteurized milk donated to a non-profit milk bank (BANKED; N = 30); milk expressed for peer-to-peer milk sharing (SHARED; N = 31); and health professional-facilitated milk sharing where donors are serologically screened and milk is dispensed raw (SCREENED; N = 30).
30592165	8	44	theme	Mean	1540:1543	arg1	values					1553:1558	Mean protein values	1540:1558	Mean protein values (95% confidence interval)	1540:1584	Mean protein values (95% confidence interval) were 1.5 g/dL (1.4, 1.6) for BANKED, 1.4 g/dL (1.3, 1.5) for MOM, 1.6 g/dL (1.5, 1.7) for SCREENED, and 1.5 g/dL (1.4, 1.6) for SHARED, which was not significantly different (p = .081).
30592165	3	45	theme	bacteria	562:569	arg1	composition					571:581	the macronutrient, antimicrobial protein, and bacteria composition	516:581	the macronutrient, antimicrobial protein, and bacteria composition in expressed human milk acquired via commerce-free arrangements	516:645	The study objective was to evaluate the macronutrient, antimicrobial protein, and bacteria composition in expressed human milk acquired via commerce-free arrangements.
30592165	3	46	from	composition	571:581	arg1	milk					602:605	expressed human milk	586:605	expressed human milk acquired via commerce-free arrangements	586:645	The study objective was to evaluate the macronutrient, antimicrobial protein, and bacteria composition in expressed human milk acquired via commerce-free arrangements.
30592165	2	47	theme	bioactive	357:365	arg1	profiles					367:374	the nutrient and bioactive profiles	340:374	the nutrient and bioactive profiles of expressed human milk exchanged through commerce-free scenarios, including peer-to-peer milk sharing	340:477	There is a paucity of research regarding the nutrient and bioactive profiles of expressed human milk exchanged through commerce-free scenarios, including peer-to-peer milk sharing.
30592165	2	48	theme	nutrient	344:351	arg1	profiles					367:374	the nutrient and bioactive profiles	340:374	the nutrient and bioactive profiles of expressed human milk exchanged through commerce-free scenarios, including peer-to-peer milk sharing	340:477	There is a paucity of research regarding the nutrient and bioactive profiles of expressed human milk exchanged through commerce-free scenarios, including peer-to-peer milk sharing.
30592165	7	49	theme	differences	1406:1416	arg1	evidence					1394:1401	no evidence	1391:1401	no evidence of differences	1391:1416	There was no evidence of differences by groups (p > .05) in lactose, fat, water, lysozyme activity, sIgA activity, aerobic bacteria, coliforms, and S. aureus.
30592165	8	50	dep	1.5 g/dL	1690:1697	arg1	1.6					1705:1707	1.6	1705:1707	1.6	1705:1707	Mean protein values (95% confidence interval) were 1.5 g/dL (1.4, 1.6) for BANKED, 1.4 g/dL (1.3, 1.5) for MOM, 1.6 g/dL (1.5, 1.7) for SCREENED, and 1.5 g/dL (1.4, 1.6) for SHARED, which was not significantly different (p = .081).
30592165	7	51	theme	lysozyme	1462:1469	arg1	activity					1471:1478	lysozyme activity	1462:1478	lysozyme activity	1462:1478	There was no evidence of differences by groups (p > .05) in lactose, fat, water, lysozyme activity, sIgA activity, aerobic bacteria, coliforms, and S. aureus.
30592165	2	52	theme	commerce-free	418:430	arg1	milk					466:469	peer-to-peer milk	453:469	peer-to-peer milk sharing	453:477	There is a paucity of research regarding the nutrient and bioactive profiles of expressed human milk exchanged through commerce-free scenarios, including peer-to-peer milk sharing.
30592165	2	52	theme	commerce-free	418:430	arg1	scenarios					432:440	commerce-free scenarios	418:440	commerce-free scenarios	418:440	There is a paucity of research regarding the nutrient and bioactive profiles of expressed human milk exchanged through commerce-free scenarios, including peer-to-peer milk sharing.
30592165	4	53	dep	SHARED	921:926	arg1	N = 31					929:934	N = 31	929:934	N = 31	929:934	Expressed human milk samples were collected from the following commerce-free scenarios: milk expressed for a mother's or parent's own infant (MOM; N = 30); unpasteurized milk donated to a non-profit milk bank (BANKED; N = 30); milk expressed for peer-to-peer milk sharing (SHARED; N = 31); and health professional-facilitated milk sharing where donors are serologically screened and milk is dispensed raw (SCREENED; N = 30).
30592165	7	54	theme	aerobic	1496:1502	arg1	bacteria					1504:1511	aerobic bacteria	1496:1511	aerobic bacteria	1496:1511	There was no evidence of differences by groups (p > .05) in lactose, fat, water, lysozyme activity, sIgA activity, aerobic bacteria, coliforms, and S. aureus.
30592165	9	55	theme	growing	1801:1807	arg1	literature					1809:1818	growing literature	1801:1818	growing literature on the risks and benefits of uncompensated, peer-to-peer milk sharing	1801:1888	This research contributes to growing literature on the risks and benefits of uncompensated, peer-to-peer milk sharing.
30592165	4	56	theme	following	701:709	arg1	infant					782:787	parent's own infant	769:787	parent's own infant (MOM; N = 30)	769:801	Expressed human milk samples were collected from the following commerce-free scenarios: milk expressed for a mother's or parent's own infant (MOM; N = 30); unpasteurized milk donated to a non-profit milk bank (BANKED; N = 30); milk expressed for peer-to-peer milk sharing (SHARED; N = 31); and health professional-facilitated milk sharing where donors are serologically screened and milk is dispensed raw (SCREENED; N = 30).
30592165	4	56	theme	following	701:709	arg1	milk					736:739	milk	736:739	milk expressed for a mother's	736:764	Expressed human milk samples were collected from the following commerce-free scenarios: milk expressed for a mother's or parent's own infant (MOM; N = 30); unpasteurized milk donated to a non-profit milk bank (BANKED; N = 30); milk expressed for peer-to-peer milk sharing (SHARED; N = 31); and health professional-facilitated milk sharing where donors are serologically screened and milk is dispensed raw (SCREENED; N = 30).
30592165	4	56	theme	following	701:709	arg1	scenarios					725:733	the following commerce-free scenarios	697:733	the following commerce-free scenarios: milk expressed for a mother's or parent's own infant (MOM; N = 30)	697:801	Expressed human milk samples were collected from the following commerce-free scenarios: milk expressed for a mother's or parent's own infant (MOM; N = 30); unpasteurized milk donated to a non-profit milk bank (BANKED; N = 30); milk expressed for peer-to-peer milk sharing (SHARED; N = 31); and health professional-facilitated milk sharing where donors are serologically screened and milk is dispensed raw (SCREENED; N = 30).
30592165	0	57	theme	antimicrobial	28:40	arg1	proteins					42:49	antimicrobial proteins	28:49	antimicrobial proteins	28:49	A pilot study on nutrients, antimicrobial proteins, and bacteria in commerce-free models for exchanging expressed human milk in the USA.
30592165	6	58	theme	52/121	1297:1302	arg1	samples					1304:1310	52/121 samples	1297:1310	52/121 samples	1297:1310	No bacterial growth was observed in 52/121 samples, and 15/121 had growth greater than 5.0 log colony-forming units/mL.
30592165	4	59	theme	milk	847:850	arg1	BANKED					858:863	BANKED	858:863	BANKED	858:863	Expressed human milk samples were collected from the following commerce-free scenarios: milk expressed for a mother's or parent's own infant (MOM; N = 30); unpasteurized milk donated to a non-profit milk bank (BANKED; N = 30); milk expressed for peer-to-peer milk sharing (SHARED; N = 31); and health professional-facilitated milk sharing where donors are serologically screened and milk is dispensed raw (SCREENED; N = 30).
30592165	4	59	theme	milk	847:850	arg1	bank					852:855	a non-profit milk bank	834:855	a non-profit milk bank (BANKED; N = 30)	834:872	Expressed human milk samples were collected from the following commerce-free scenarios: milk expressed for a mother's or parent's own infant (MOM; N = 30); unpasteurized milk donated to a non-profit milk bank (BANKED; N = 30); milk expressed for peer-to-peer milk sharing (SHARED; N = 31); and health professional-facilitated milk sharing where donors are serologically screened and milk is dispensed raw (SCREENED; N = 30).
30592165	6	60	contain	had	1324:1326	arg1	15/121					1317:1322	15/121	1317:1322	15/121	1317:1322	No bacterial growth was observed in 52/121 samples, and 15/121 had growth greater than 5.0 log colony-forming units/mL.
30592165	6	60	contain	had	1324:1326	arg2	units/mL					1371:1378	growth greater than 5.0 log colony-forming units/mL	1328:1378	growth greater than 5.0 log colony-forming units/mL	1328:1378	No bacterial growth was observed in 52/121 samples, and 15/121 had growth greater than 5.0 log colony-forming units/mL.
30592165	4	61	theme	human	658:662	arg1	samples					669:675	Expressed human milk samples	648:675	Expressed human milk samples	648:675	Expressed human milk samples were collected from the following commerce-free scenarios: milk expressed for a mother's or parent's own infant (MOM; N = 30); unpasteurized milk donated to a non-profit milk bank (BANKED; N = 30); milk expressed for peer-to-peer milk sharing (SHARED; N = 31); and health professional-facilitated milk sharing where donors are serologically screened and milk is dispensed raw (SCREENED; N = 30).
30592165	1	62	theme	peer-to-peer	272:283	arg1	milk					285:288	peer-to-peer milk sharing	272:296	peer-to-peer milk sharing	272:296	Expressed human milk can be donated or sold through a variety of channels, including human milk banks, corporations or individuals, or peer-to-peer milk sharing.
30592165	9	63	theme	uncompensated	1849:1861	arg1	milk					1877:1880	uncompensated, peer-to-peer milk	1849:1880	uncompensated, peer-to-peer milk sharing	1849:1888	This research contributes to growing literature on the risks and benefits of uncompensated, peer-to-peer milk sharing.
30592165	4	64	theme	professional-facilitated	949:972	arg1	milk					974:977	health professional-facilitated milk	942:977	health professional-facilitated milk sharing where donors are serologically screened	942:1025	Expressed human milk samples were collected from the following commerce-free scenarios: milk expressed for a mother's or parent's own infant (MOM; N = 30); unpasteurized milk donated to a non-profit milk bank (BANKED; N = 30); milk expressed for peer-to-peer milk sharing (SHARED; N = 31); and health professional-facilitated milk sharing where donors are serologically screened and milk is dispensed raw (SCREENED; N = 30).
30592165	3	65	theme	human	596:600	arg1	milk					602:605	expressed human milk	586:605	expressed human milk acquired via commerce-free arrangements	586:645	The study objective was to evaluate the macronutrient, antimicrobial protein, and bacteria composition in expressed human milk acquired via commerce-free arrangements.
30592165	4	66	dep	scenarios	725:733	arg1	MOM					790:792	MOM	790:792	MOM	790:792	Expressed human milk samples were collected from the following commerce-free scenarios: milk expressed for a mother's or parent's own infant (MOM; N = 30); unpasteurized milk donated to a non-profit milk bank (BANKED; N = 30); milk expressed for peer-to-peer milk sharing (SHARED; N = 31); and health professional-facilitated milk sharing where donors are serologically screened and milk is dispensed raw (SCREENED; N = 30).
30592165	4	66	dep	scenarios	725:733	arg1	infant					782:787	parent's own infant	769:787	parent's own infant (MOM; N = 30)	769:801	Expressed human milk samples were collected from the following commerce-free scenarios: milk expressed for a mother's or parent's own infant (MOM; N = 30); unpasteurized milk donated to a non-profit milk bank (BANKED; N = 30); milk expressed for peer-to-peer milk sharing (SHARED; N = 31); and health professional-facilitated milk sharing where donors are serologically screened and milk is dispensed raw (SCREENED; N = 30).
30592165	4	66	dep	scenarios	725:733	arg1	milk					736:739	milk	736:739	milk expressed for a mother's	736:764	Expressed human milk samples were collected from the following commerce-free scenarios: milk expressed for a mother's or parent's own infant (MOM; N = 30); unpasteurized milk donated to a non-profit milk bank (BANKED; N = 30); milk expressed for peer-to-peer milk sharing (SHARED; N = 31); and health professional-facilitated milk sharing where donors are serologically screened and milk is dispensed raw (SCREENED; N = 30).
30592165	4	66	dep	scenarios	725:733	arg1	scenarios					725:733	the following commerce-free scenarios	697:733	the following commerce-free scenarios: milk expressed for a mother's or parent's own infant (MOM; N = 30)	697:801	Expressed human milk samples were collected from the following commerce-free scenarios: milk expressed for a mother's or parent's own infant (MOM; N = 30); unpasteurized milk donated to a non-profit milk bank (BANKED; N = 30); milk expressed for peer-to-peer milk sharing (SHARED; N = 31); and health professional-facilitated milk sharing where donors are serologically screened and milk is dispensed raw (SCREENED; N = 30).
30592165	6	67	located	observed	1285:1292	arg1	samples					1304:1310	52/121 samples	1297:1310	52/121 samples	1297:1310	No bacterial growth was observed in 52/121 samples, and 15/121 had growth greater than 5.0 log colony-forming units/mL.
30592165	6	67	located	observed	1285:1292	arg2	growth					1274:1279	No bacterial growth	1261:1279	No bacterial growth	1261:1279	No bacterial growth was observed in 52/121 samples, and 15/121 had growth greater than 5.0 log colony-forming units/mL.
30592165	6	68	dep	units/mL	1371:1378	arg1	log					1352:1354	log	1352:1354	log	1352:1354	No bacterial growth was observed in 52/121 samples, and 15/121 had growth greater than 5.0 log colony-forming units/mL.
30592165	4	69	theme	commerce-free	711:723	arg1	infant					782:787	parent's own infant	769:787	parent's own infant (MOM; N = 30)	769:801	Expressed human milk samples were collected from the following commerce-free scenarios: milk expressed for a mother's or parent's own infant (MOM; N = 30); unpasteurized milk donated to a non-profit milk bank (BANKED; N = 30); milk expressed for peer-to-peer milk sharing (SHARED; N = 31); and health professional-facilitated milk sharing where donors are serologically screened and milk is dispensed raw (SCREENED; N = 30).
30592165	4	69	theme	commerce-free	711:723	arg1	milk					736:739	milk	736:739	milk expressed for a mother's	736:764	Expressed human milk samples were collected from the following commerce-free scenarios: milk expressed for a mother's or parent's own infant (MOM; N = 30); unpasteurized milk donated to a non-profit milk bank (BANKED; N = 30); milk expressed for peer-to-peer milk sharing (SHARED; N = 31); and health professional-facilitated milk sharing where donors are serologically screened and milk is dispensed raw (SCREENED; N = 30).
30592165	4	69	theme	commerce-free	711:723	arg1	scenarios					725:733	the following commerce-free scenarios	697:733	the following commerce-free scenarios: milk expressed for a mother's or parent's own infant (MOM; N = 30)	697:801	Expressed human milk samples were collected from the following commerce-free scenarios: milk expressed for a mother's or parent's own infant (MOM; N = 30); unpasteurized milk donated to a non-profit milk bank (BANKED; N = 30); milk expressed for peer-to-peer milk sharing (SHARED; N = 31); and health professional-facilitated milk sharing where donors are serologically screened and milk is dispensed raw (SCREENED; N = 30).
30592165	4	70	theme	sharing	912:918	arg1	milk					907:910	peer-to-peer milk sharing	894:918	peer-to-peer milk sharing	894:918	Expressed human milk samples were collected from the following commerce-free scenarios: milk expressed for a mother's or parent's own infant (MOM; N = 30); unpasteurized milk donated to a non-profit milk bank (BANKED; N = 30); milk expressed for peer-to-peer milk sharing (SHARED; N = 31); and health professional-facilitated milk sharing where donors are serologically screened and milk is dispensed raw (SCREENED; N = 30).
30592165	5	71	theme	A	1182:1182	arg1	activity					1190:1197	immunoglobulin A (IgA) activity	1167:1197	immunoglobulin A (IgA) activity	1167:1197	Analyses were conducted for total protein, lactose, percent fat and water, lysozyme activity, immunoglobulin A (IgA) activity, total aerobic bacteria, coliform, and Staphylococcus aureus.
30592165	4	72	dep	N = 30	1064:1069	arg1	SCREENED					1054:1061	SCREENED	1054:1061	SCREENED	1054:1061	Expressed human milk samples were collected from the following commerce-free scenarios: milk expressed for a mother's or parent's own infant (MOM; N = 30); unpasteurized milk donated to a non-profit milk bank (BANKED; N = 30); milk expressed for peer-to-peer milk sharing (SHARED; N = 31); and health professional-facilitated milk sharing where donors are serologically screened and milk is dispensed raw (SCREENED; N = 30).
30592165	3	73	theme	study	484:488	arg1	objective					490:498	The study objective	480:498	The study objective	480:498	The study objective was to evaluate the macronutrient, antimicrobial protein, and bacteria composition in expressed human milk acquired via commerce-free arrangements.
30592165	6	74	theme	colony-forming	1356:1369	arg1	units/mL					1371:1378	growth greater than 5.0 log colony-forming units/mL	1328:1378	growth greater than 5.0 log colony-forming units/mL	1328:1378	No bacterial growth was observed in 52/121 samples, and 15/121 had growth greater than 5.0 log colony-forming units/mL.
30592165	5	75	theme	percent	1125:1131	arg1	fat					1133:1135	percent fat	1125:1135	percent fat	1125:1135	Analyses were conducted for total protein, lactose, percent fat and water, lysozyme activity, immunoglobulin A (IgA) activity, total aerobic bacteria, coliform, and Staphylococcus aureus.
30592165	8	76	dep	1.4 g/dL	1623:1630	arg1	1.5					1638:1640	1.5	1638:1640	1.5	1638:1640	Mean protein values (95% confidence interval) were 1.5 g/dL (1.4, 1.6) for BANKED, 1.4 g/dL (1.3, 1.5) for MOM, 1.6 g/dL (1.5, 1.7) for SCREENED, and 1.5 g/dL (1.4, 1.6) for SHARED, which was not significantly different (p = .081).
30592165	3	77	theme	expressed	586:594	arg1	milk					602:605	expressed human milk	586:605	expressed human milk acquired via commerce-free arrangements	586:645	The study objective was to evaluate the macronutrient, antimicrobial protein, and bacteria composition in expressed human milk acquired via commerce-free arrangements.
30592165	4	78	theme	health	942:947	arg1	milk					974:977	health professional-facilitated milk	942:977	health professional-facilitated milk sharing where donors are serologically screened	942:1025	Expressed human milk samples were collected from the following commerce-free scenarios: milk expressed for a mother's or parent's own infant (MOM; N = 30); unpasteurized milk donated to a non-profit milk bank (BANKED; N = 30); milk expressed for peer-to-peer milk sharing (SHARED; N = 31); and health professional-facilitated milk sharing where donors are serologically screened and milk is dispensed raw (SCREENED; N = 30).
30592165	1	79	theme	channels	202:209	arg1	corporations					240:251	corporations	240:251	corporations	240:251	Expressed human milk can be donated or sold through a variety of channels, including human milk banks, corporations or individuals, or peer-to-peer milk sharing.
30592165	1	79	theme	channels	202:209	arg1	milk					285:288	peer-to-peer milk sharing	272:296	peer-to-peer milk sharing	272:296	Expressed human milk can be donated or sold through a variety of channels, including human milk banks, corporations or individuals, or peer-to-peer milk sharing.
30592165	1	79	theme	channels	202:209	arg1	banks					233:237	human milk banks	222:237	human milk banks	222:237	Expressed human milk can be donated or sold through a variety of channels, including human milk banks, corporations or individuals, or peer-to-peer milk sharing.
30592165	1	79	theme	channels	202:209	arg1	channels					202:209	channels	202:209	channels	202:209	Expressed human milk can be donated or sold through a variety of channels, including human milk banks, corporations or individuals, or peer-to-peer milk sharing.
30592165	1	79	theme	channels	202:209	arg1	variety					191:197	a variety	189:197	a variety	189:197	Expressed human milk can be donated or sold through a variety of channels, including human milk banks, corporations or individuals, or peer-to-peer milk sharing.
30527986	2	0	theme	×	508:508	arg1	design					525:530	a 6 × 6 Latin square design	504:530	a 6 × 6 Latin square design in which each experimental period consisted of 5 d of continuous abomasal infusion followed by 2 d of rest	504:637	Six rumen-fistulated, second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were randomly assigned to a 6 × 6 Latin square design in which each experimental period consisted of 5 d of continuous abomasal infusion followed by 2 d of rest.
30527986	17	1	theme	N	3045:3045	arg1	efficiency					3047:3056	improved milk N efficiency	3031:3056	improved milk N efficiency	3031:3056	Regardless of protein level, GG promoted energy retention and improved milk N efficiency, but not through increased milk protein yield.
30527986	4	2	theme	fatty	1081:1085	arg1	constituents					1092:1103	major fatty acid constituents	1075:1103	major fatty acid constituents	1075:1103	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	4	2	theme	fatty	1081:1085	arg1	acid					1139:1142	palmitic, oleic, and linoleic acid	1109:1142	palmitic, oleic, and linoleic acid	1109:1142	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	1	3	theme	N	358:358	arg1	partitioning					360:371	N partitioning	358:371	N partitioning of dairy cattle	358:387	This study tested the effects of energy from glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates at low (LMP) and high (HMP) metabolizable protein levels on whole-body energy and N partitioning of dairy cattle.
30527986	12	4	theme	N	2415:2415	arg1	intake					2417:2422	N intake	2415:2422	N intake	2415:2422	Infusion of LG decreased N intake but did not affect milk N efficiency or N retention.
30527986	8	5	theme	Infusing	1742:1749	arg1	GG					1751:1752	GG	1751:1752	GG	1751:1752	Infusing GG or LG at the HMP level did not affect milk production differently than at the LMP level.
30527986	16	6	theme	N	2848:2848	arg1	intake					2850:2855	N intake	2848:2855	N intake	2848:2855	The HMP level increased N intake, milk N output, and plasma urea concentration, tended to increase N retention, and decreased milk N efficiency.
30527986	4	7	theme	palm	1055:1058	arg1	olein					1060:1064	palm olein	1055:1064	palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid)	1055:1143	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	9	8	theme	glucose	1919:1925	arg1	concentrations					1939:1952	plasma glucose and insulin concentrations	1912:1952	plasma glucose and insulin concentrations	1912:1952	Infusion of GG stimulated energy retention in body tissue, increased plasma glucose and insulin concentrations, decreased lipogenic metabolites in plasma, and decreased milk fat yield and milk energy output.
30527986	1	9	theme	lipogenic	238:246	arg1	substrates					265:274	glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates	210:274	glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates	210:274	This study tested the effects of energy from glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates at low (LMP) and high (HMP) metabolizable protein levels on whole-body energy and N partitioning of dairy cattle.
30527986	16	10	theme	plasma	2877:2882	arg1	concentration					2889:2901	plasma urea concentration	2877:2901	plasma urea concentration	2877:2901	The HMP level increased N intake, milk N output, and plasma urea concentration, tended to increase N retention, and decreased milk N efficiency.
30527986	10	11	theme	N	2086:2086	arg1	efficiency					2088:2097	milk N efficiency	2081:2097	milk N efficiency	2081:2097	Nitrogen intake decreased and milk N efficiency increased in response to GG, and N retention was not affected.
30527986	3	12	theme	metabolizable	802:814	arg1	requirements					824:835	metabolizable protein requirements	802:835	metabolizable protein requirements	802:835	A total mixed ration consisting of 42% corn silage, 31% grass silage, and 27% concentrate (dry matter basis) was formulated to meet 100 and 83% of net energy and metabolizable protein requirements, respectively, and was fed at 90% of ad libitum intake by individual cow.
30527986	2	13	theme	rest	634:637	arg1	d					629:629	2 d	627:629	2 d of rest	627:637	Six rumen-fistulated, second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were randomly assigned to a 6 × 6 Latin square design in which each experimental period consisted of 5 d of continuous abomasal infusion followed by 2 d of rest.
30527986	5	14	theme	N	1413:1413	arg1	balance					1415:1421	energy and N balance	1402:1421	balance	1415:1421	The experiment was conducted in climate respiration chambers to determine energy and N balance in conjunction with milk production and composition, nutrient digestibility, and plasma constituents.
30527986	1	15	theme	cattle	382:387	arg1	energy					347:352	whole-body energy	336:352	whole-body energy	336:352	This study tested the effects of energy from glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates at low (LMP) and high (HMP) metabolizable protein levels on whole-body energy and N partitioning of dairy cattle.
30527986	1	15	theme	cattle	382:387	arg1	partitioning					360:371	N partitioning	358:371	N partitioning of dairy cattle	358:387	This study tested the effects of energy from glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates at low (LMP) and high (HMP) metabolizable protein levels on whole-body energy and N partitioning of dairy cattle.
30527986	9	16	theme	insulin	1931:1937	arg1	concentrations					1939:1952	plasma glucose and insulin concentrations	1912:1952	plasma glucose and insulin concentrations	1912:1952	Infusion of GG stimulated energy retention in body tissue, increased plasma glucose and insulin concentrations, decreased lipogenic metabolites in plasma, and decreased milk fat yield and milk energy output.
30527986	2	17	theme	Latin	512:516	arg1	design					525:530	a 6 × 6 Latin square design	504:530	a 6 × 6 Latin square design in which each experimental period consisted of 5 d of continuous abomasal infusion followed by 2 d of rest	504:637	Six rumen-fistulated, second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were randomly assigned to a 6 × 6 Latin square design in which each experimental period consisted of 5 d of continuous abomasal infusion followed by 2 d of rest.
30527986	4	18	theme	infusion	920:927	arg1	treatments					929:938	Abomasal infusion treatments	911:938	Abomasal infusion treatments	911:938	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	5	19	with	conjunction	1426:1436	arg1	composition					1463:1473	composition	1463:1473	composition	1463:1473	The experiment was conducted in climate respiration chambers to determine energy and N balance in conjunction with milk production and composition, nutrient digestibility, and plasma constituents.
30527986	5	19	with	conjunction	1426:1436	arg1	production					1448:1457	milk production	1443:1457	milk production	1443:1457	The experiment was conducted in climate respiration chambers to determine energy and N balance in conjunction with milk production and composition, nutrient digestibility, and plasma constituents.
30527986	17	20	theme	protein	2983:2989	arg1	level					2991:2995	protein level	2983:2995	protein level	2983:2995	Regardless of protein level, GG promoted energy retention and improved milk N efficiency, but not through increased milk protein yield.
30527986	4	21	theme	oleic	1119:1123	arg1	acid					1139:1142	palmitic, oleic, and linoleic acid	1109:1142	palmitic, oleic, and linoleic acid	1109:1142	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	4	21	theme	oleic	1119:1123	arg1	constituents					1092:1103	major fatty acid constituents	1075:1103	major fatty acid constituents	1075:1103	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	4	22	theme	g/d	1238:1240	arg1	basis					1003:1007	digestible energy basis	985:1007	digestible energy basis	985:1007	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	4	22	theme	g/d	1238:1240	arg1	infusions					1198:1206	isoenergetic infusions	1185:1206	isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG)	1185:1265	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	4	22	theme	g/d	1238:1240	arg1	infusions					974:982	isoenergetic infusions	961:982	isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C)	961:1179	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	4	22	theme	g/d	1238:1240	arg1	saline					945:950	saline	945:950	saline (LMP-C)	945:958	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	4	22	theme	g/d	1238:1240	arg1	LMP-C					953:957	LMP-C	953:957	LMP-C	953:957	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	4	22	theme	g/d	1238:1240	arg1	g/d					1274:1276	676 g/d	1270:1276	676 g/d of palm olein + 844 g/d of essential AA (HMP-LG)	1270:1325	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	4	23	dep	olein	1060:1064	arg1	acid					1139:1142	palmitic, oleic, and linoleic acid	1109:1142	palmitic, oleic, and linoleic acid	1109:1142	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	4	23	dep	olein	1060:1064	arg1	constituents					1092:1103	major fatty acid constituents	1075:1103	major fatty acid constituents	1075:1103	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	8	24	theme	HMP	1767:1769	arg1	level					1771:1775	the HMP level	1763:1775	the HMP level	1763:1775	Infusing GG or LG at the HMP level did not affect milk production differently than at the LMP level.
30527986	11	25	theme	plasma	2284:2289	arg1	triacylglycerides					2291:2307	plasma triacylglycerides	2284:2307	plasma triacylglycerides	2284:2307	Infusion of LG tended to increase metabolizable energy intake, increased milk fat yield and milk energy output, increased plasma triacylglycerides and long-chain fatty acid concentrations, and had no effect on energy retention.
30527986	4	26	theme	essential	1245:1253	arg1	HMP-GG					1259:1264	HMP-GG	1259:1264	HMP-GG	1259:1264	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	4	26	theme	essential	1245:1253	arg1	AA					1255:1256	essential AA	1245:1256	essential AA (HMP-GG)	1245:1265	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	9	27	from	metabolites	1975:1985	arg1	plasma					1990:1995	plasma	1990:1995	plasma	1990:1995	Infusion of GG stimulated energy retention in body tissue, increased plasma glucose and insulin concentrations, decreased lipogenic metabolites in plasma, and decreased milk fat yield and milk energy output.
30527986	13	28	theme	metabolizable	2559:2571	arg1	intake					2580:2585	gross and metabolizable energy intake	2549:2585	gross and metabolizable energy intake	2549:2585	Compared with the LMP level, the HMP level increased dry matter intake, gross and metabolizable energy intake, and total milk, fat, protein, and lactose yields.
30527986	7	29	theme	total	1702:1706	arg1	yields					1734:1739	total milk, protein, or lactose yields	1702:1739	total milk, protein, or lactose yields	1702:1739	Furthermore, GG or LG did not affect total milk, protein, or lactose yields.
30527986	3	30	theme	mixed	648:652	arg1	ration					654:659	A total mixed ration	640:659	A total mixed ration consisting of 42% corn silage, 31% grass silage, and 27% concentrate (dry matter basis)	640:747	A total mixed ration consisting of 42% corn silage, 31% grass silage, and 27% concentrate (dry matter basis) was formulated to meet 100 and 83% of net energy and metabolizable protein requirements, respectively, and was fed at 90% of ad libitum intake by individual cow.
30527986	0	31	theme	postrumen	118:126	arg1	substrates					153:162	postrumen glucogenic and lipogenic substrates	118:162	postrumen glucogenic and lipogenic substrates	118:162	Energy and nitrogen partitioning in dairy cows at low or high metabolizable protein levels is affected differently by postrumen glucogenic and lipogenic substrates.
30527986	1	32	theme	protein	318:324	arg1	levels					326:331	low (LMP) and high (HMP) metabolizable protein levels	279:331	low (LMP) and high (HMP) metabolizable protein levels	279:331	This study tested the effects of energy from glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates at low (LMP) and high (HMP) metabolizable protein levels on whole-body energy and N partitioning of dairy cattle.
30527986	4	33	theme	olein	1286:1290	arg1	basis					1003:1007	digestible energy basis	985:1007	digestible energy basis	985:1007	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	4	33	theme	olein	1286:1290	arg1	infusions					1198:1206	isoenergetic infusions	1185:1206	isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG)	1185:1265	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	4	33	theme	olein	1286:1290	arg1	infusions					974:982	isoenergetic infusions	961:982	isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C)	961:1179	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	4	33	theme	olein	1286:1290	arg1	saline					945:950	saline	945:950	saline (LMP-C)	945:958	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	4	33	theme	olein	1286:1290	arg1	LMP-C					953:957	LMP-C	953:957	LMP-C	953:957	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	4	33	theme	olein	1286:1290	arg1	g/d					1274:1276	676 g/d	1270:1276	676 g/d of palm olein + 844 g/d of essential AA (HMP-LG)	1270:1325	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	14	34	theme	energy	2724:2729	arg1	retention					2731:2739	total energy retention	2718:2739	total energy retention	2718:2739	Milk energy output increased at the HMP level, and protein level did not affect total energy retention.
30527986	13	35	theme	dry	2530:2532	arg1	intake					2541:2546	dry matter intake	2530:2546	dry matter intake	2530:2546	Compared with the LMP level, the HMP level increased dry matter intake, gross and metabolizable energy intake, and total milk, fat, protein, and lactose yields.
30527986	2	36	theme	continuous	586:595	arg1	infusion					606:613	continuous abomasal infusion	586:613	continuous abomasal infusion	586:613	Six rumen-fistulated, second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were randomly assigned to a 6 × 6 Latin square design in which each experimental period consisted of 5 d of continuous abomasal infusion followed by 2 d of rest.
30527986	6	37	theme	total	1580:1584	arg1	intake					1599:1604	total gross energy intake	1580:1604	total gross energy intake from the diet plus infusions	1580:1633	Infusion of GG and LG decreased dry matter intake, but total gross energy intake from the diet plus infusions was not affected by GG or LG.
30527986	4	38	theme	palmitic	1109:1116	arg1	acid					1139:1142	palmitic, oleic, and linoleic acid	1109:1142	palmitic, oleic, and linoleic acid	1109:1142	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	4	38	theme	palmitic	1109:1116	arg1	constituents					1092:1103	major fatty acid constituents	1075:1103	major fatty acid constituents	1075:1103	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	1	39	from	effects	187:193	arg1	energy					347:352	whole-body energy	336:352	whole-body energy	336:352	This study tested the effects of energy from glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates at low (LMP) and high (HMP) metabolizable protein levels on whole-body energy and N partitioning of dairy cattle.
30527986	1	39	from	effects	187:193	arg1	levels					326:331	low (LMP) and high (HMP) metabolizable protein levels	279:331	low (LMP) and high (HMP) metabolizable protein levels	279:331	This study tested the effects of energy from glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates at low (LMP) and high (HMP) metabolizable protein levels on whole-body energy and N partitioning of dairy cattle.
30527986	1	39	from	effects	187:193	arg1	substrates					265:274	glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates	210:274	glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates	210:274	This study tested the effects of energy from glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates at low (LMP) and high (HMP) metabolizable protein levels on whole-body energy and N partitioning of dairy cattle.
30527986	1	39	from	effects	187:193	arg1	partitioning					360:371	N partitioning	358:371	N partitioning of dairy cattle	358:387	This study tested the effects of energy from glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates at low (LMP) and high (HMP) metabolizable protein levels on whole-body energy and N partitioning of dairy cattle.
30527986	17	40	theme	increased	3075:3083	arg1	yield					3098:3102	increased milk protein yield	3075:3102	increased milk protein yield	3075:3102	Regardless of protein level, GG promoted energy retention and improved milk N efficiency, but not through increased milk protein yield.
30527986	2	41	dep	consisted	566:574	arg1	followed					615:622	followed	615:622	followed by 2 d of rest	615:637	Six rumen-fistulated, second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were randomly assigned to a 6 × 6 Latin square design in which each experimental period consisted of 5 d of continuous abomasal infusion followed by 2 d of rest.
30527986	13	42	theme	intake	2541:2546	arg1	yields					2630:2635	dry matter intake, gross and metabolizable energy intake, and total milk, fat, protein, and lactose yields	2530:2635	dry matter intake, gross and metabolizable energy intake, and total milk, fat, protein, and lactose yields	2530:2635	Compared with the LMP level, the HMP level increased dry matter intake, gross and metabolizable energy intake, and total milk, fat, protein, and lactose yields.
30527986	4	43	theme	isoenergetic	961:972	arg1	basis					1003:1007	digestible energy basis	985:1007	digestible energy basis	985:1007	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	4	43	theme	isoenergetic	961:972	arg1	infusions					974:982	isoenergetic infusions	961:982	isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C)	961:1179	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	11	44	theme	metabolizable	2196:2208	arg1	intake					2217:2222	metabolizable energy intake	2196:2222	metabolizable energy intake	2196:2222	Infusion of LG tended to increase metabolizable energy intake, increased milk fat yield and milk energy output, increased plasma triacylglycerides and long-chain fatty acid concentrations, and had no effect on energy retention.
30527986	3	45	theme	42	675:676	arg1	%					677:677	%	677:677	%	677:677	A total mixed ration consisting of 42% corn silage, 31% grass silage, and 27% concentrate (dry matter basis) was formulated to meet 100 and 83% of net energy and metabolizable protein requirements, respectively, and was fed at 90% of ad libitum intake by individual cow.
30527986	17	46	theme	protein	3090:3096	arg1	yield					3098:3102	increased milk protein yield	3075:3102	increased milk protein yield	3075:3102	Regardless of protein level, GG promoted energy retention and improved milk N efficiency, but not through increased milk protein yield.
30527986	3	47	theme	%	694:694	arg1	silage					702:707	31% grass silage	692:707	31% grass silage	692:707	A total mixed ration consisting of 42% corn silage, 31% grass silage, and 27% concentrate (dry matter basis) was formulated to meet 100 and 83% of net energy and metabolizable protein requirements, respectively, and was fed at 90% of ad libitum intake by individual cow.
30527986	10	48	dep	GG	2124:2125	arg1	response					2112:2119	response	2112:2119	response	2112:2119	Nitrogen intake decreased and milk N efficiency increased in response to GG, and N retention was not affected.
30527986	2	49	theme	dairy	447:451	arg1	cows					453:456	Six rumen-fistulated, second-lactation Holstein-Friesian dairy cows	390:456	cows	453:456	Six rumen-fistulated, second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were randomly assigned to a 6 × 6 Latin square design in which each experimental period consisted of 5 d of continuous abomasal infusion followed by 2 d of rest.
30527986	12	50	theme	N	2448:2448	arg1	efficiency					2450:2459	milk N efficiency	2443:2459	milk N efficiency	2443:2459	Infusion of LG decreased N intake but did not affect milk N efficiency or N retention.
30527986	6	51	theme	matter	1561:1566	arg1	intake					1568:1573	dry matter intake	1557:1573	dry matter intake	1557:1573	Infusion of GG and LG decreased dry matter intake, but total gross energy intake from the diet plus infusions was not affected by GG or LG.
30527986	0	52	theme	low	50:52	arg1	levels					84:89	low or high metabolizable protein levels	50:89	low or high metabolizable protein levels	50:89	Energy and nitrogen partitioning in dairy cows at low or high metabolizable protein levels is affected differently by postrumen glucogenic and lipogenic substrates.
30527986	1	53	theme	low	279:281	arg1	levels					326:331	low (LMP) and high (HMP) metabolizable protein levels	279:331	low (LMP) and high (HMP) metabolizable protein levels	279:331	This study tested the effects of energy from glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates at low (LMP) and high (HMP) metabolizable protein levels on whole-body energy and N partitioning of dairy cattle.
30527986	9	54	theme	fat	2017:2019	arg1	yield					2021:2025	milk fat yield	2012:2025	milk fat yield	2012:2025	Infusion of GG stimulated energy retention in body tissue, increased plasma glucose and insulin concentrations, decreased lipogenic metabolites in plasma, and decreased milk fat yield and milk energy output.
30527986	14	55	theme	protein	2689:2695	arg1	level					2697:2701	protein level	2689:2701	protein level	2689:2701	Milk energy output increased at the HMP level, and protein level did not affect total energy retention.
30527986	3	56	theme	%	716:716	arg1	basis					742:746	dry matter basis	731:746	dry matter basis	731:746	A total mixed ration consisting of 42% corn silage, 31% grass silage, and 27% concentrate (dry matter basis) was formulated to meet 100 and 83% of net energy and metabolizable protein requirements, respectively, and was fed at 90% of ad libitum intake by individual cow.
30527986	3	56	theme	%	716:716	arg1	concentrate					718:728	27% concentrate	714:728	27% concentrate (dry matter basis)	714:747	A total mixed ration consisting of 42% corn silage, 31% grass silage, and 27% concentrate (dry matter basis) was formulated to meet 100 and 83% of net energy and metabolizable protein requirements, respectively, and was fed at 90% of ad libitum intake by individual cow.
30527986	2	57	theme	second-lactation	412:427	arg1	cows					453:456	Six rumen-fistulated, second-lactation Holstein-Friesian dairy cows	390:456	cows	453:456	Six rumen-fistulated, second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were randomly assigned to a 6 × 6 Latin square design in which each experimental period consisted of 5 d of continuous abomasal infusion followed by 2 d of rest.
30527986	0	58	from	nitrogen	11:18	arg1	cows					42:45	dairy cows	36:45	dairy cows	36:45	Energy and nitrogen partitioning in dairy cows at low or high metabolizable protein levels is affected differently by postrumen glucogenic and lipogenic substrates.
30527986	2	59	theme	infusion	606:613	arg1	d					581:581	5 d	579:581	5 d of continuous abomasal infusion	579:613	Six rumen-fistulated, second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were randomly assigned to a 6 × 6 Latin square design in which each experimental period consisted of 5 d of continuous abomasal infusion followed by 2 d of rest.
30527986	0	60	theme	high	57:60	arg1	levels					84:89	low or high metabolizable protein levels	50:89	low or high metabolizable protein levels	50:89	Energy and nitrogen partitioning in dairy cows at low or high metabolizable protein levels is affected differently by postrumen glucogenic and lipogenic substrates.
30527986	5	61	from	balance	1415:1421	arg1	conjunction					1426:1436	conjunction	1426:1436	conjunction with milk production and composition, nutrient digestibility, and plasma constituents	1426:1522	The experiment was conducted in climate respiration chambers to determine energy and N balance in conjunction with milk production and composition, nutrient digestibility, and plasma constituents.
30527986	2	62	theme	±	462:462	arg1	d					467:467	97 ± 13 d	459:467	97 ± 13 d in milk	459:475	Six rumen-fistulated, second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were randomly assigned to a 6 × 6 Latin square design in which each experimental period consisted of 5 d of continuous abomasal infusion followed by 2 d of rest.
30527986	0	63	from	Energy	0:5	arg1	cows					42:45	dairy cows	36:45	dairy cows	36:45	Energy and nitrogen partitioning in dairy cows at low or high metabolizable protein levels is affected differently by postrumen glucogenic and lipogenic substrates.
30527986	10	64	theme	N	2132:2132	arg1	retention					2134:2142	N retention	2132:2142	N retention	2132:2142	Nitrogen intake decreased and milk N efficiency increased in response to GG, and N retention was not affected.
30527986	3	65	theme	corn	679:682	arg1	silage					684:689	42% corn silage	675:689	42% corn silage	675:689	A total mixed ration consisting of 42% corn silage, 31% grass silage, and 27% concentrate (dry matter basis) was formulated to meet 100 and 83% of net energy and metabolizable protein requirements, respectively, and was fed at 90% of ad libitum intake by individual cow.
30527986	0	66	theme	protein	76:82	arg1	levels					84:89	low or high metabolizable protein levels	50:89	low or high metabolizable protein levels	50:89	Energy and nitrogen partitioning in dairy cows at low or high metabolizable protein levels is affected differently by postrumen glucogenic and lipogenic substrates.
30527986	9	67	theme	energy	2036:2041	arg1	output					2043:2048	milk energy output	2031:2048	milk energy output	2031:2048	Infusion of GG stimulated energy retention in body tissue, increased plasma glucose and insulin concentrations, decreased lipogenic metabolites in plasma, and decreased milk fat yield and milk energy output.
30527986	14	68	theme	HMP	2674:2676	arg1	level					2678:2682	the HMP level	2670:2682	the HMP level	2670:2682	Milk energy output increased at the HMP level, and protein level did not affect total energy retention.
30527986	3	69	theme	matter	735:740	arg1	basis					742:746	dry matter basis	731:746	dry matter basis	731:746	A total mixed ration consisting of 42% corn silage, 31% grass silage, and 27% concentrate (dry matter basis) was formulated to meet 100 and 83% of net energy and metabolizable protein requirements, respectively, and was fed at 90% of ad libitum intake by individual cow.
30527986	3	69	theme	matter	735:740	arg1	concentrate					718:728	27% concentrate	714:728	27% concentrate (dry matter basis)	714:747	A total mixed ration consisting of 42% corn silage, 31% grass silage, and 27% concentrate (dry matter basis) was formulated to meet 100 and 83% of net energy and metabolizable protein requirements, respectively, and was fed at 90% of ad libitum intake by individual cow.
30527986	6	70	from	diet	1615:1618	arg1	intake					1599:1604	total gross energy intake	1580:1604	total gross energy intake from the diet plus infusions	1580:1633	Infusion of GG and LG decreased dry matter intake, but total gross energy intake from the diet plus infusions was not affected by GG or LG.
30527986	4	71	theme	essential	1305:1313	arg1	AA					1315:1316	essential AA	1305:1316	essential AA (HMP-LG)	1305:1325	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	4	71	theme	essential	1305:1313	arg1	HMP-LG					1319:1324	HMP-LG	1319:1324	HMP-LG	1319:1324	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	4	72	theme	1,319	1013:1017	arg1	g/d					1019:1021	1,319 g/d	1013:1021	1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C)	1013:1179	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	3	73	theme	libitum	877:883	arg1	intake					885:890	ad libitum intake	874:890	ad libitum intake	874:890	A total mixed ration consisting of 42% corn silage, 31% grass silage, and 27% concentrate (dry matter basis) was formulated to meet 100 and 83% of net energy and metabolizable protein requirements, respectively, and was fed at 90% of ad libitum intake by individual cow.
30527986	9	74	from	retention	1876:1884	arg1	tissue					1894:1899	body tissue	1889:1899	body tissue	1889:1899	Infusion of GG stimulated energy retention in body tissue, increased plasma glucose and insulin concentrations, decreased lipogenic metabolites in plasma, and decreased milk fat yield and milk energy output.
30527986	4	75	theme	isoenergetic	1185:1196	arg1	infusions					1198:1206	isoenergetic infusions	1185:1206	isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG)	1185:1265	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	11	76	contain	had	2355:2357	arg1	Infusion					2162:2169	Infusion	2162:2169	Infusion of LG	2162:2175	Infusion of LG tended to increase metabolizable energy intake, increased milk fat yield and milk energy output, increased plasma triacylglycerides and long-chain fatty acid concentrations, and had no effect on energy retention.
30527986	11	76	contain	had	2355:2357	arg2	effect					2362:2367	no effect	2359:2367	no effect	2359:2367	Infusion of LG tended to increase metabolizable energy intake, increased milk fat yield and milk energy output, increased plasma triacylglycerides and long-chain fatty acid concentrations, and had no effect on energy retention.
30527986	9	77	theme	milk	2012:2015	arg1	yield					2021:2025	milk fat yield	2012:2025	milk fat yield	2012:2025	Infusion of GG stimulated energy retention in body tissue, increased plasma glucose and insulin concentrations, decreased lipogenic metabolites in plasma, and decreased milk fat yield and milk energy output.
30527986	4	78	theme	energy	996:1001	arg1	basis					1003:1007	digestible energy basis	985:1007	digestible energy basis	985:1007	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	4	78	theme	energy	996:1001	arg1	infusions					974:982	isoenergetic infusions	961:982	isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C)	961:1179	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	6	79	theme	energy	1592:1597	arg1	intake					1599:1604	total gross energy intake	1580:1604	total gross energy intake from the diet plus infusions	1580:1633	Infusion of GG and LG decreased dry matter intake, but total gross energy intake from the diet plus infusions was not affected by GG or LG.
30527986	4	80	theme	AA	1170:1171	arg1	LMP-GG					1035:1040	LMP-GG	1035:1040	LMP-GG	1035:1040	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	4	80	theme	AA	1170:1171	arg1	g/d					1153:1155	844 g/d	1149:1155	844 g/d of essential AA (HMP-C)	1149:1179	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	4	80	theme	AA	1170:1171	arg1	glucose					1026:1032	glucose	1026:1032	glucose (LMP-GG)	1026:1041	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	4	80	theme	AA	1170:1171	arg1	g/d					1048:1050	676 g/d	1044:1050	676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid)	1044:1143	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	2	81	dep	cows	453:456	arg1	d					467:467	97 ± 13 d	459:467	97 ± 13 d in milk	459:475	Six rumen-fistulated, second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were randomly assigned to a 6 × 6 Latin square design in which each experimental period consisted of 5 d of continuous abomasal infusion followed by 2 d of rest.
30527986	9	82	theme	lipogenic	1965:1973	arg1	metabolites					1975:1985	lipogenic metabolites	1965:1985	lipogenic metabolites in plasma	1965:1995	Infusion of GG stimulated energy retention in body tissue, increased plasma glucose and insulin concentrations, decreased lipogenic metabolites in plasma, and decreased milk fat yield and milk energy output.
30527986	11	83	theme	fat	2240:2242	arg1	yield					2244:2248	milk fat yield	2235:2248	milk fat yield	2235:2248	Infusion of LG tended to increase metabolizable energy intake, increased milk fat yield and milk energy output, increased plasma triacylglycerides and long-chain fatty acid concentrations, and had no effect on energy retention.
30527986	2	84	theme	rumen-fistulated	394:409	arg1	cows					453:456	Six rumen-fistulated, second-lactation Holstein-Friesian dairy cows	390:456	cows	453:456	Six rumen-fistulated, second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were randomly assigned to a 6 × 6 Latin square design in which each experimental period consisted of 5 d of continuous abomasal infusion followed by 2 d of rest.
30527986	16	85	theme	milk	2858:2861	arg1	output					2865:2870	milk N output	2858:2870	milk N output	2858:2870	The HMP level increased N intake, milk N output, and plasma urea concentration, tended to increase N retention, and decreased milk N efficiency.
30527986	18	86	theme	extra	3132:3136	arg1	intake					3145:3150	extra energy intake	3132:3150	extra energy intake	3132:3150	Infusion of LG partitioned extra energy intake into milk and had no effect on milk N efficiency.
30527986	11	87	theme	fatty	2324:2328	arg1	acid					2330:2333	long-chain fatty acid	2313:2333	long-chain fatty acid concentrations	2313:2348	Infusion of LG tended to increase metabolizable energy intake, increased milk fat yield and milk energy output, increased plasma triacylglycerides and long-chain fatty acid concentrations, and had no effect on energy retention.
30527986	7	88	theme	lactose	1726:1732	arg1	yields					1734:1739	total milk, protein, or lactose yields	1702:1739	total milk, protein, or lactose yields	1702:1739	Furthermore, GG or LG did not affect total milk, protein, or lactose yields.
30527986	4	89	dep	acid	1139:1142	arg1	LMP-LG					1067:1072	LMP-LG	1067:1072	LMP-LG	1067:1072	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	18	90	theme	LG	3117:3118	arg1	Infusion					3105:3112	Infusion	3105:3112	Infusion of LG	3105:3118	Infusion of LG partitioned extra energy intake into milk and had no effect on milk N efficiency.
30527986	4	91	theme	glucose	1224:1230	arg1	g/d					1217:1219	1,319 g/d	1211:1219	1,319 g/d of glucose	1211:1230	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	13	92	theme	total	2592:2596	arg1	milk					2598:2601	total milk	2592:2601	total milk	2592:2601	Compared with the LMP level, the HMP level increased dry matter intake, gross and metabolizable energy intake, and total milk, fat, protein, and lactose yields.
30527986	13	93	theme	milk	2598:2601	arg1	yields					2630:2635	dry matter intake, gross and metabolizable energy intake, and total milk, fat, protein, and lactose yields	2530:2635	dry matter intake, gross and metabolizable energy intake, and total milk, fat, protein, and lactose yields	2530:2635	Compared with the LMP level, the HMP level increased dry matter intake, gross and metabolizable energy intake, and total milk, fat, protein, and lactose yields.
30527986	1	94	dep	high	293:296	arg1	HMP					299:301	HMP	299:301	HMP	299:301	This study tested the effects of energy from glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates at low (LMP) and high (HMP) metabolizable protein levels on whole-body energy and N partitioning of dairy cattle.
30527986	5	95	theme	plasma	1504:1509	arg1	constituents					1511:1522	plasma constituents	1504:1522	plasma constituents	1504:1522	The experiment was conducted in climate respiration chambers to determine energy and N balance in conjunction with milk production and composition, nutrient digestibility, and plasma constituents.
30527986	1	96	theme	glucogenic	210:219	arg1	substrates					265:274	glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates	210:274	glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates	210:274	This study tested the effects of energy from glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates at low (LMP) and high (HMP) metabolizable protein levels on whole-body energy and N partitioning of dairy cattle.
30527986	3	97	theme	net	787:789	arg1	energy					791:796	net energy	787:796	net energy	787:796	A total mixed ration consisting of 42% corn silage, 31% grass silage, and 27% concentrate (dry matter basis) was formulated to meet 100 and 83% of net energy and metabolizable protein requirements, respectively, and was fed at 90% of ad libitum intake by individual cow.
30527986	13	98	theme	fat	2604:2606	arg1	yields					2630:2635	dry matter intake, gross and metabolizable energy intake, and total milk, fat, protein, and lactose yields	2530:2635	dry matter intake, gross and metabolizable energy intake, and total milk, fat, protein, and lactose yields	2530:2635	Compared with the LMP level, the HMP level increased dry matter intake, gross and metabolizable energy intake, and total milk, fat, protein, and lactose yields.
30527986	9	99	theme	body	1889:1892	arg1	tissue					1894:1899	body tissue	1889:1899	body tissue	1889:1899	Infusion of GG stimulated energy retention in body tissue, increased plasma glucose and insulin concentrations, decreased lipogenic metabolites in plasma, and decreased milk fat yield and milk energy output.
30527986	0	100	dep	Energy	0:5	arg1	partitioning					20:31	partitioning	20:31	partitioning	20:31	Energy and nitrogen partitioning in dairy cows at low or high metabolizable protein levels is affected differently by postrumen glucogenic and lipogenic substrates.
30527986	13	101	theme	intake	2580:2585	arg1	yields					2630:2635	dry matter intake, gross and metabolizable energy intake, and total milk, fat, protein, and lactose yields	2530:2635	dry matter intake, gross and metabolizable energy intake, and total milk, fat, protein, and lactose yields	2530:2635	Compared with the LMP level, the HMP level increased dry matter intake, gross and metabolizable energy intake, and total milk, fat, protein, and lactose yields.
30527986	17	102	theme	milk	3040:3043	arg1	efficiency					3047:3056	improved milk N efficiency	3031:3056	improved milk N efficiency	3031:3056	Regardless of protein level, GG promoted energy retention and improved milk N efficiency, but not through increased milk protein yield.
30527986	1	103	from	substrates	265:274	arg1	energy					198:203	energy	198:203	energy from glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates at low (LMP) and high (HMP) metabolizable protein levels	198:331	This study tested the effects of energy from glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates at low (LMP) and high (HMP) metabolizable protein levels on whole-body energy and N partitioning of dairy cattle.
30527986	1	103	from	substrates	265:274	arg1	effects					187:193	the effects	183:193	the effects of energy from glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates at low (LMP) and high (HMP) metabolizable protein levels on whole-body energy and N partitioning of dairy cattle	183:387	This study tested the effects of energy from glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates at low (LMP) and high (HMP) metabolizable protein levels on whole-body energy and N partitioning of dairy cattle.
30527986	8	104	from	level	1771:1775	arg1	GG					1751:1752	GG	1751:1752	GG	1751:1752	Infusing GG or LG at the HMP level did not affect milk production differently than at the LMP level.
30527986	8	104	from	level	1771:1775	arg1	LG					1757:1758	LG	1757:1758	LG	1757:1758	Infusing GG or LG at the HMP level did not affect milk production differently than at the LMP level.
30527986	4	105	theme	g/d	1217:1219	arg1	basis					1003:1007	digestible energy basis	985:1007	digestible energy basis	985:1007	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	4	105	theme	g/d	1217:1219	arg1	infusions					1198:1206	isoenergetic infusions	1185:1206	isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG)	1185:1265	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	4	105	theme	g/d	1217:1219	arg1	infusions					974:982	isoenergetic infusions	961:982	isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C)	961:1179	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	4	105	theme	g/d	1217:1219	arg1	saline					945:950	saline	945:950	saline (LMP-C)	945:958	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	4	105	theme	g/d	1217:1219	arg1	LMP-C					953:957	LMP-C	953:957	LMP-C	953:957	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	4	105	theme	g/d	1217:1219	arg1	g/d					1274:1276	676 g/d	1270:1276	676 g/d of palm olein + 844 g/d of essential AA (HMP-LG)	1270:1325	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	3	106	theme	protein	816:822	arg1	requirements					824:835	metabolizable protein requirements	802:835	metabolizable protein requirements	802:835	A total mixed ration consisting of 42% corn silage, 31% grass silage, and 27% concentrate (dry matter basis) was formulated to meet 100 and 83% of net energy and metabolizable protein requirements, respectively, and was fed at 90% of ad libitum intake by individual cow.
30527986	16	107	theme	HMP	2828:2830	arg1	level					2832:2836	The HMP level	2824:2836	The HMP level	2824:2836	The HMP level increased N intake, milk N output, and plasma urea concentration, tended to increase N retention, and decreased milk N efficiency.
30527986	4	108	theme	major	1075:1079	arg1	constituents					1092:1103	major fatty acid constituents	1075:1103	major fatty acid constituents	1075:1103	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	4	108	theme	major	1075:1079	arg1	acid					1139:1142	palmitic, oleic, and linoleic acid	1109:1142	palmitic, oleic, and linoleic acid	1109:1142	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	9	109	theme	plasma	1912:1917	arg1	glucose					1919:1925	plasma glucose	1912:1925	plasma glucose	1912:1925	Infusion of GG stimulated energy retention in body tissue, increased plasma glucose and insulin concentrations, decreased lipogenic metabolites in plasma, and decreased milk fat yield and milk energy output.
30527986	4	110	theme	Abomasal	911:918	arg1	treatments					929:938	Abomasal infusion treatments	911:938	Abomasal infusion treatments	911:938	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	5	111	theme	climate	1360:1366	arg1	chambers					1380:1387	climate respiration chambers	1360:1387	climate respiration chambers	1360:1387	The experiment was conducted in climate respiration chambers to determine energy and N balance in conjunction with milk production and composition, nutrient digestibility, and plasma constituents.
30527986	1	112	theme	dairy	376:380	arg1	cattle					382:387	dairy cattle	376:387	dairy cattle	376:387	This study tested the effects of energy from glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates at low (LMP) and high (HMP) metabolizable protein levels on whole-body energy and N partitioning of dairy cattle.
30527986	16	113	theme	milk	2950:2953	arg1	efficiency					2957:2966	milk N efficiency	2950:2966	milk N efficiency	2950:2966	The HMP level increased N intake, milk N output, and plasma urea concentration, tended to increase N retention, and decreased milk N efficiency.
30527986	2	114	theme	square	518:523	arg1	design					525:530	a 6 × 6 Latin square design	504:530	a 6 × 6 Latin square design in which each experimental period consisted of 5 d of continuous abomasal infusion followed by 2 d of rest	504:637	Six rumen-fistulated, second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were randomly assigned to a 6 × 6 Latin square design in which each experimental period consisted of 5 d of continuous abomasal infusion followed by 2 d of rest.
30527986	16	115	theme	N	2955:2955	arg1	efficiency					2957:2966	milk N efficiency	2950:2966	milk N efficiency	2950:2966	The HMP level increased N intake, milk N output, and plasma urea concentration, tended to increase N retention, and decreased milk N efficiency.
30527986	6	116	from	infusions	1625:1633	arg1	intake					1599:1604	total gross energy intake	1580:1604	total gross energy intake from the diet plus infusions	1580:1633	Infusion of GG and LG decreased dry matter intake, but total gross energy intake from the diet plus infusions was not affected by GG or LG.
30527986	6	117	theme	GG	1537:1538	arg1	Infusion					1525:1532	Infusion	1525:1532	Infusion of GG and LG	1525:1545	Infusion of GG and LG decreased dry matter intake, but total gross energy intake from the diet plus infusions was not affected by GG or LG.
30527986	4	118	theme	olein	1060:1064	arg1	LMP-GG					1035:1040	LMP-GG	1035:1040	LMP-GG	1035:1040	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	4	118	theme	olein	1060:1064	arg1	g/d					1153:1155	844 g/d	1149:1155	844 g/d of essential AA (HMP-C)	1149:1179	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	4	118	theme	olein	1060:1064	arg1	glucose					1026:1032	glucose	1026:1032	glucose (LMP-GG)	1026:1041	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	4	118	theme	olein	1060:1064	arg1	g/d					1048:1050	676 g/d	1044:1050	676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid)	1044:1143	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	16	119	theme	N	2923:2923	arg1	retention					2925:2933	N retention	2923:2933	N retention	2923:2933	The HMP level increased N intake, milk N output, and plasma urea concentration, tended to increase N retention, and decreased milk N efficiency.
30527986	1	120	from	levels	326:331	arg1	energy					198:203	energy	198:203	energy from glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates at low (LMP) and high (HMP) metabolizable protein levels	198:331	This study tested the effects of energy from glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates at low (LMP) and high (HMP) metabolizable protein levels on whole-body energy and N partitioning of dairy cattle.
30527986	1	120	from	levels	326:331	arg1	effects					187:193	the effects	183:193	the effects of energy from glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates at low (LMP) and high (HMP) metabolizable protein levels on whole-body energy and N partitioning of dairy cattle	183:387	This study tested the effects of energy from glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates at low (LMP) and high (HMP) metabolizable protein levels on whole-body energy and N partitioning of dairy cattle.
30527986	18	121	theme	N	3188:3188	arg1	efficiency					3190:3199	milk N efficiency	3183:3199	milk N efficiency	3183:3199	Infusion of LG partitioned extra energy intake into milk and had no effect on milk N efficiency.
30527986	11	122	theme	milk	2254:2257	arg1	output					2266:2271	milk energy output	2254:2271	milk energy output	2254:2271	Infusion of LG tended to increase metabolizable energy intake, increased milk fat yield and milk energy output, increased plasma triacylglycerides and long-chain fatty acid concentrations, and had no effect on energy retention.
30527986	4	123	theme	AA	1255:1256	arg1	g/d					1238:1240	844 g/d	1234:1240	844 g/d of essential AA (HMP-GG)	1234:1265	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	5	124	theme	energy	1402:1407	arg1	balance					1415:1421	energy and N balance	1402:1421	balance	1415:1421	The experiment was conducted in climate respiration chambers to determine energy and N balance in conjunction with milk production and composition, nutrient digestibility, and plasma constituents.
30527986	1	125	dep	glucose	222:228	arg1	GG					231:232	GG	231:232	GG	231:232	This study tested the effects of energy from glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates at low (LMP) and high (HMP) metabolizable protein levels on whole-body energy and N partitioning of dairy cattle.
30527986	11	126	theme	milk	2235:2238	arg1	yield					2244:2248	milk fat yield	2235:2248	milk fat yield	2235:2248	Infusion of LG tended to increase metabolizable energy intake, increased milk fat yield and milk energy output, increased plasma triacylglycerides and long-chain fatty acid concentrations, and had no effect on energy retention.
30527986	7	127	theme	protein	1714:1720	arg1	yields					1734:1739	total milk, protein, or lactose yields	1702:1739	total milk, protein, or lactose yields	1702:1739	Furthermore, GG or LG did not affect total milk, protein, or lactose yields.
30527986	9	128	theme	energy	1869:1874	arg1	retention					1876:1884	energy retention	1869:1884	energy retention in body tissue	1869:1899	Infusion of GG stimulated energy retention in body tissue, increased plasma glucose and insulin concentrations, decreased lipogenic metabolites in plasma, and decreased milk fat yield and milk energy output.
30527986	0	129	theme	glucogenic	128:137	arg1	substrates					153:162	postrumen glucogenic and lipogenic substrates	118:162	postrumen glucogenic and lipogenic substrates	118:162	Energy and nitrogen partitioning in dairy cows at low or high metabolizable protein levels is affected differently by postrumen glucogenic and lipogenic substrates.
30527986	1	130	theme	metabolizable	304:316	arg1	levels					326:331	low (LMP) and high (HMP) metabolizable protein levels	279:331	low (LMP) and high (HMP) metabolizable protein levels	279:331	This study tested the effects of energy from glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates at low (LMP) and high (HMP) metabolizable protein levels on whole-body energy and N partitioning of dairy cattle.
30527986	18	131	contain	had	3166:3168	arg1	Infusion					3105:3112	Infusion	3105:3112	Infusion of LG	3105:3118	Infusion of LG partitioned extra energy intake into milk and had no effect on milk N efficiency.
30527986	18	131	contain	had	3166:3168	arg2	effect					3173:3178	no effect	3170:3178	no effect	3170:3178	Infusion of LG partitioned extra energy intake into milk and had no effect on milk N efficiency.
30527986	1	132	theme	energy	198:203	arg1	effects					187:193	the effects	183:193	the effects of energy from glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates at low (LMP) and high (HMP) metabolizable protein levels on whole-body energy and N partitioning of dairy cattle	183:387	This study tested the effects of energy from glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates at low (LMP) and high (HMP) metabolizable protein levels on whole-body energy and N partitioning of dairy cattle.
30527986	4	133	theme	linoleic	1130:1137	arg1	acid					1139:1142	palmitic, oleic, and linoleic acid	1109:1142	palmitic, oleic, and linoleic acid	1109:1142	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	4	133	theme	linoleic	1130:1137	arg1	constituents					1092:1103	major fatty acid constituents	1075:1103	major fatty acid constituents	1075:1103	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	1	134	dep	glucogenic	210:219	arg1	olein					254:258	palm olein	249:258	palm olein; LG	249:262	This study tested the effects of energy from glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates at low (LMP) and high (HMP) metabolizable protein levels on whole-body energy and N partitioning of dairy cattle.
30527986	1	134	dep	glucogenic	210:219	arg1	glucose					222:228	glucose	222:228	glucose	222:228	This study tested the effects of energy from glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates at low (LMP) and high (HMP) metabolizable protein levels on whole-body energy and N partitioning of dairy cattle.
30527986	2	135	theme	abomasal	597:604	arg1	infusion					606:613	continuous abomasal infusion	586:613	continuous abomasal infusion	586:613	Six rumen-fistulated, second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were randomly assigned to a 6 × 6 Latin square design in which each experimental period consisted of 5 d of continuous abomasal infusion followed by 2 d of rest.
30527986	7	136	theme	milk	1708:1711	arg1	yields					1734:1739	total milk, protein, or lactose yields	1702:1739	total milk, protein, or lactose yields	1702:1739	Furthermore, GG or LG did not affect total milk, protein, or lactose yields.
30527986	17	137	theme	energy	3010:3015	arg1	retention					3017:3025	energy retention	3010:3025	energy retention	3010:3025	Regardless of protein level, GG promoted energy retention and improved milk N efficiency, but not through increased milk protein yield.
30527986	4	138	theme	acid	1087:1090	arg1	constituents					1092:1103	major fatty acid constituents	1075:1103	major fatty acid constituents	1075:1103	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	4	138	theme	acid	1087:1090	arg1	acid					1139:1142	palmitic, oleic, and linoleic acid	1109:1142	palmitic, oleic, and linoleic acid	1109:1142	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	0	139	theme	lipogenic	143:151	arg1	substrates					153:162	postrumen glucogenic and lipogenic substrates	118:162	postrumen glucogenic and lipogenic substrates	118:162	Energy and nitrogen partitioning in dairy cows at low or high metabolizable protein levels is affected differently by postrumen glucogenic and lipogenic substrates.
30527986	13	140	theme	gross	2549:2553	arg1	intake					2580:2585	gross and metabolizable energy intake	2549:2585	gross and metabolizable energy intake	2549:2585	Compared with the LMP level, the HMP level increased dry matter intake, gross and metabolizable energy intake, and total milk, fat, protein, and lactose yields.
30527986	4	141	theme	g/d	1298:1300	arg1	basis					1003:1007	digestible energy basis	985:1007	digestible energy basis	985:1007	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	4	141	theme	g/d	1298:1300	arg1	infusions					1198:1206	isoenergetic infusions	1185:1206	isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG)	1185:1265	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	4	141	theme	g/d	1298:1300	arg1	infusions					974:982	isoenergetic infusions	961:982	isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C)	961:1179	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	4	141	theme	g/d	1298:1300	arg1	saline					945:950	saline	945:950	saline (LMP-C)	945:958	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	4	141	theme	g/d	1298:1300	arg1	LMP-C					953:957	LMP-C	953:957	LMP-C	953:957	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	4	141	theme	g/d	1298:1300	arg1	g/d					1274:1276	676 g/d	1270:1276	676 g/d of palm olein + 844 g/d of essential AA (HMP-LG)	1270:1325	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	14	142	theme	total	2718:2722	arg1	retention					2731:2739	total energy retention	2718:2739	total energy retention	2718:2739	Milk energy output increased at the HMP level, and protein level did not affect total energy retention.
30527986	13	143	theme	matter	2534:2539	arg1	intake					2541:2546	dry matter intake	2530:2546	dry matter intake	2530:2546	Compared with the LMP level, the HMP level increased dry matter intake, gross and metabolizable energy intake, and total milk, fat, protein, and lactose yields.
30527986	10	144	theme	milk	2081:2084	arg1	efficiency					2088:2097	milk N efficiency	2081:2097	milk N efficiency	2081:2097	Nitrogen intake decreased and milk N efficiency increased in response to GG, and N retention was not affected.
30527986	3	145	theme	total	642:646	arg1	ration					654:659	A total mixed ration	640:659	A total mixed ration consisting of 42% corn silage, 31% grass silage, and 27% concentrate (dry matter basis)	640:747	A total mixed ration consisting of 42% corn silage, 31% grass silage, and 27% concentrate (dry matter basis) was formulated to meet 100 and 83% of net energy and metabolizable protein requirements, respectively, and was fed at 90% of ad libitum intake by individual cow.
30527986	1	146	theme	whole-body	336:345	arg1	energy					347:352	whole-body energy	336:352	whole-body energy	336:352	This study tested the effects of energy from glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates at low (LMP) and high (HMP) metabolizable protein levels on whole-body energy and N partitioning of dairy cattle.
30527986	5	147	theme	milk	1443:1446	arg1	production					1448:1457	milk production	1443:1457	milk production	1443:1457	The experiment was conducted in climate respiration chambers to determine energy and N balance in conjunction with milk production and composition, nutrient digestibility, and plasma constituents.
30527986	17	148	theme	milk	3085:3088	arg1	yield					3098:3102	increased milk protein yield	3075:3102	increased milk protein yield	3075:3102	Regardless of protein level, GG promoted energy retention and improved milk N efficiency, but not through increased milk protein yield.
30527986	0	149	from	levels	84:89	arg1	nitrogen					11:18	nitrogen	11:18	nitrogen	11:18	Energy and nitrogen partitioning in dairy cows at low or high metabolizable protein levels is affected differently by postrumen glucogenic and lipogenic substrates.
30527986	0	149	from	levels	84:89	arg1	Energy					0:5	Energy	0:5	Energy	0:5	Energy and nitrogen partitioning in dairy cows at low or high metabolizable protein levels is affected differently by postrumen glucogenic and lipogenic substrates.
30527986	8	150	theme	milk	1792:1795	arg1	production					1797:1806	milk production	1792:1806	milk production	1792:1806	Infusing GG or LG at the HMP level did not affect milk production differently than at the LMP level.
30527986	11	151	theme	energy	2210:2215	arg1	intake					2217:2222	metabolizable energy intake	2196:2222	metabolizable energy intake	2196:2222	Infusion of LG tended to increase metabolizable energy intake, increased milk fat yield and milk energy output, increased plasma triacylglycerides and long-chain fatty acid concentrations, and had no effect on energy retention.
30527986	3	152	theme	%	677:677	arg1	silage					684:689	42% corn silage	675:689	42% corn silage	675:689	A total mixed ration consisting of 42% corn silage, 31% grass silage, and 27% concentrate (dry matter basis) was formulated to meet 100 and 83% of net energy and metabolizable protein requirements, respectively, and was fed at 90% of ad libitum intake by individual cow.
30527986	4	153	theme	palm	1281:1284	arg1	olein					1286:1290	palm olein	1281:1290	palm olein	1281:1290	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	3	154	theme	grass	696:700	arg1	silage					702:707	31% grass silage	692:707	31% grass silage	692:707	A total mixed ration consisting of 42% corn silage, 31% grass silage, and 27% concentrate (dry matter basis) was formulated to meet 100 and 83% of net energy and metabolizable protein requirements, respectively, and was fed at 90% of ad libitum intake by individual cow.
30527986	13	155	theme	HMP	2510:2512	arg1	level					2514:2518	the HMP level	2506:2518	the HMP level	2506:2518	Compared with the LMP level, the HMP level increased dry matter intake, gross and metabolizable energy intake, and total milk, fat, protein, and lactose yields.
30527986	2	156	theme	Holstein-Friesian	429:445	arg1	cows					453:456	Six rumen-fistulated, second-lactation Holstein-Friesian dairy cows	390:456	cows	453:456	Six rumen-fistulated, second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were randomly assigned to a 6 × 6 Latin square design in which each experimental period consisted of 5 d of continuous abomasal infusion followed by 2 d of rest.
30527986	1	157	dep	olein	254:258	arg1	LG					261:262	LG	261:262	palm olein; LG	249:262	This study tested the effects of energy from glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates at low (LMP) and high (HMP) metabolizable protein levels on whole-body energy and N partitioning of dairy cattle.
30527986	6	158	theme	dry	1557:1559	arg1	intake					1568:1573	dry matter intake	1557:1573	dry matter intake	1557:1573	Infusion of GG and LG decreased dry matter intake, but total gross energy intake from the diet plus infusions was not affected by GG or LG.
30527986	4	159	theme	g/d	1153:1155	arg1	g/d					1019:1021	1,319 g/d	1013:1021	1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C)	1013:1179	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	0	160	theme	metabolizable	62:74	arg1	levels					84:89	low or high metabolizable protein levels	50:89	low or high metabolizable protein levels	50:89	Energy and nitrogen partitioning in dairy cows at low or high metabolizable protein levels is affected differently by postrumen glucogenic and lipogenic substrates.
30527986	2	161	theme	experimental	546:557	arg1	period					559:564	each experimental period	541:564	each experimental period	541:564	Six rumen-fistulated, second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were randomly assigned to a 6 × 6 Latin square design in which each experimental period consisted of 5 d of continuous abomasal infusion followed by 2 d of rest.
30527986	6	162	theme	LG	1544:1545	arg1	Infusion					1525:1532	Infusion	1525:1532	Infusion of GG and LG	1525:1545	Infusion of GG and LG decreased dry matter intake, but total gross energy intake from the diet plus infusions was not affected by GG or LG.
30527986	3	163	theme	31	692:693	arg1	%					694:694	%	694:694	%	694:694	A total mixed ration consisting of 42% corn silage, 31% grass silage, and 27% concentrate (dry matter basis) was formulated to meet 100 and 83% of net energy and metabolizable protein requirements, respectively, and was fed at 90% of ad libitum intake by individual cow.
30527986	16	164	theme	urea	2884:2887	arg1	concentration					2889:2901	plasma urea concentration	2877:2901	plasma urea concentration	2877:2901	The HMP level increased N intake, milk N output, and plasma urea concentration, tended to increase N retention, and decreased milk N efficiency.
30527986	9	165	theme	milk	2031:2034	arg1	output					2043:2048	milk energy output	2031:2048	milk energy output	2031:2048	Infusion of GG stimulated energy retention in body tissue, increased plasma glucose and insulin concentrations, decreased lipogenic metabolites in plasma, and decreased milk fat yield and milk energy output.
30527986	3	166	theme	27	714:715	arg1	%					716:716	%	716:716	%	716:716	A total mixed ration consisting of 42% corn silage, 31% grass silage, and 27% concentrate (dry matter basis) was formulated to meet 100 and 83% of net energy and metabolizable protein requirements, respectively, and was fed at 90% of ad libitum intake by individual cow.
30527986	13	167	theme	LMP	2495:2497	arg1	level					2499:2503	the LMP level	2491:2503	the LMP level	2491:2503	Compared with the LMP level, the HMP level increased dry matter intake, gross and metabolizable energy intake, and total milk, fat, protein, and lactose yields.
30527986	15	168	theme	Heat	2742:2745	arg1	production					2747:2756	Heat production	2742:2756	Heat production	2742:2756	Heat production increased at the HMP level, but only when GG and LG were infused.
30527986	4	169	theme	g/d	1019:1021	arg1	basis					1003:1007	digestible energy basis	985:1007	digestible energy basis	985:1007	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	4	169	theme	g/d	1019:1021	arg1	infusions					1198:1206	isoenergetic infusions	1185:1206	isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG)	1185:1265	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	4	169	theme	g/d	1019:1021	arg1	infusions					974:982	isoenergetic infusions	961:982	isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C)	961:1179	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	4	169	theme	g/d	1019:1021	arg1	saline					945:950	saline	945:950	saline (LMP-C)	945:958	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	4	169	theme	g/d	1019:1021	arg1	LMP-C					953:957	LMP-C	953:957	LMP-C	953:957	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	4	169	theme	g/d	1019:1021	arg1	g/d					1274:1276	676 g/d	1270:1276	676 g/d of palm olein + 844 g/d of essential AA (HMP-LG)	1270:1325	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	11	170	theme	energy	2372:2377	arg1	retention					2379:2387	energy retention	2372:2387	energy retention	2372:2387	Infusion of LG tended to increase metabolizable energy intake, increased milk fat yield and milk energy output, increased plasma triacylglycerides and long-chain fatty acid concentrations, and had no effect on energy retention.
30527986	18	171	theme	milk	3183:3186	arg1	efficiency					3190:3199	milk N efficiency	3183:3199	milk N efficiency	3183:3199	Infusion of LG partitioned extra energy intake into milk and had no effect on milk N efficiency.
30527986	1	172	theme	high	293:296	arg1	levels					326:331	low (LMP) and high (HMP) metabolizable protein levels	279:331	low (LMP) and high (HMP) metabolizable protein levels	279:331	This study tested the effects of energy from glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates at low (LMP) and high (HMP) metabolizable protein levels on whole-body energy and N partitioning of dairy cattle.
30527986	3	173	theme	ad	874:875	arg1	intake					885:890	ad libitum intake	874:890	ad libitum intake	874:890	A total mixed ration consisting of 42% corn silage, 31% grass silage, and 27% concentrate (dry matter basis) was formulated to meet 100 and 83% of net energy and metabolizable protein requirements, respectively, and was fed at 90% of ad libitum intake by individual cow.
30527986	10	174	theme	Nitrogen	2051:2058	arg1	intake					2060:2065	Nitrogen intake	2051:2065	Nitrogen intake	2051:2065	Nitrogen intake decreased and milk N efficiency increased in response to GG, and N retention was not affected.
30527986	13	175	theme	lactose	2622:2628	arg1	yields					2630:2635	dry matter intake, gross and metabolizable energy intake, and total milk, fat, protein, and lactose yields	2530:2635	dry matter intake, gross and metabolizable energy intake, and total milk, fat, protein, and lactose yields	2530:2635	Compared with the LMP level, the HMP level increased dry matter intake, gross and metabolizable energy intake, and total milk, fat, protein, and lactose yields.
30527986	4	176	theme	digestible	985:994	arg1	basis					1003:1007	digestible energy basis	985:1007	digestible energy basis	985:1007	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	4	176	theme	digestible	985:994	arg1	infusions					974:982	isoenergetic infusions	961:982	isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C)	961:1179	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	12	177	theme	N	2464:2464	arg1	retention					2466:2474	N retention	2464:2474	N retention	2464:2474	Infusion of LG decreased N intake but did not affect milk N efficiency or N retention.
30527986	11	178	theme	energy	2259:2264	arg1	output					2266:2271	milk energy output	2254:2271	milk energy output	2254:2271	Infusion of LG tended to increase metabolizable energy intake, increased milk fat yield and milk energy output, increased plasma triacylglycerides and long-chain fatty acid concentrations, and had no effect on energy retention.
30527986	3	179	theme	dry	731:733	arg1	basis					742:746	dry matter basis	731:746	dry matter basis	731:746	A total mixed ration consisting of 42% corn silage, 31% grass silage, and 27% concentrate (dry matter basis) was formulated to meet 100 and 83% of net energy and metabolizable protein requirements, respectively, and was fed at 90% of ad libitum intake by individual cow.
30527986	3	179	theme	dry	731:733	arg1	concentrate					718:728	27% concentrate	714:728	27% concentrate (dry matter basis)	714:747	A total mixed ration consisting of 42% corn silage, 31% grass silage, and 27% concentrate (dry matter basis) was formulated to meet 100 and 83% of net energy and metabolizable protein requirements, respectively, and was fed at 90% of ad libitum intake by individual cow.
30527986	4	180	theme	AA	1315:1316	arg1	olein					1286:1290	palm olein	1281:1290	palm olein	1281:1290	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	16	181	theme	N	2863:2863	arg1	output					2865:2870	milk N output	2858:2870	milk N output	2858:2870	The HMP level increased N intake, milk N output, and plasma urea concentration, tended to increase N retention, and decreased milk N efficiency.
30527986	1	182	theme	palm	249:252	arg1	olein					254:258	palm olein	249:258	palm olein; LG	249:262	This study tested the effects of energy from glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates at low (LMP) and high (HMP) metabolizable protein levels on whole-body energy and N partitioning of dairy cattle.
30527986	3	183	theme	individual	895:904	arg1	cow					906:908	individual cow	895:908	individual cow	895:908	A total mixed ration consisting of 42% corn silage, 31% grass silage, and 27% concentrate (dry matter basis) was formulated to meet 100 and 83% of net energy and metabolizable protein requirements, respectively, and was fed at 90% of ad libitum intake by individual cow.
30527986	14	184	theme	energy	2643:2648	arg1	output					2650:2655	Milk energy output	2638:2655	Milk energy output	2638:2655	Milk energy output increased at the HMP level, and protein level did not affect total energy retention.
30527986	6	185	dep	diet	1615:1618	arg1	the					1611:1613	the	1611:1613	the	1611:1613	Infusion of GG and LG decreased dry matter intake, but total gross energy intake from the diet plus infusions was not affected by GG or LG.
30527986	1	186	dep	low	279:281	arg1	LMP					284:286	LMP	284:286	LMP	284:286	This study tested the effects of energy from glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates at low (LMP) and high (HMP) metabolizable protein levels on whole-body energy and N partitioning of dairy cattle.
30527986	18	187	theme	energy	3138:3143	arg1	intake					3145:3150	extra energy intake	3132:3150	extra energy intake	3132:3150	Infusion of LG partitioned extra energy intake into milk and had no effect on milk N efficiency.
30527986	6	188	theme	gross	1586:1590	arg1	intake					1599:1604	total gross energy intake	1580:1604	total gross energy intake from the diet plus infusions	1580:1633	Infusion of GG and LG decreased dry matter intake, but total gross energy intake from the diet plus infusions was not affected by GG or LG.
30527986	4	189	theme	essential	1160:1168	arg1	HMP-C					1174:1178	HMP-C	1174:1178	HMP-C	1174:1178	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	4	189	theme	essential	1160:1168	arg1	AA					1170:1171	essential AA	1160:1171	essential AA (HMP-C)	1160:1179	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	0	190	theme	dairy	36:40	arg1	cows					42:45	dairy cows	36:45	dairy cows	36:45	Energy and nitrogen partitioning in dairy cows at low or high metabolizable protein levels is affected differently by postrumen glucogenic and lipogenic substrates.
30527986	3	191	theme	intake	885:890	arg1	intake					885:890	ad libitum intake	874:890	ad libitum intake	874:890	A total mixed ration consisting of 42% corn silage, 31% grass silage, and 27% concentrate (dry matter basis) was formulated to meet 100 and 83% of net energy and metabolizable protein requirements, respectively, and was fed at 90% of ad libitum intake by individual cow.
30527986	3	191	theme	intake	885:890	arg1	%					869:869	90%	867:869	90% of ad libitum intake	867:890	A total mixed ration consisting of 42% corn silage, 31% grass silage, and 27% concentrate (dry matter basis) was formulated to meet 100 and 83% of net energy and metabolizable protein requirements, respectively, and was fed at 90% of ad libitum intake by individual cow.
30527986	13	192	theme	protein	2609:2615	arg1	yields					2630:2635	dry matter intake, gross and metabolizable energy intake, and total milk, fat, protein, and lactose yields	2530:2635	dry matter intake, gross and metabolizable energy intake, and total milk, fat, protein, and lactose yields	2530:2635	Compared with the LMP level, the HMP level increased dry matter intake, gross and metabolizable energy intake, and total milk, fat, protein, and lactose yields.
30527986	4	193	theme	g/d	1048:1050	arg1	g/d					1019:1021	1,319 g/d	1013:1021	1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C)	1013:1179	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	11	194	theme	acid	2330:2333	arg1	concentrations					2335:2348	long-chain fatty acid concentrations	2313:2348	long-chain fatty acid concentrations	2313:2348	Infusion of LG tended to increase metabolizable energy intake, increased milk fat yield and milk energy output, increased plasma triacylglycerides and long-chain fatty acid concentrations, and had no effect on energy retention.
30527986	9	195	theme	GG	1855:1856	arg1	Infusion					1843:1850	Infusion	1843:1850	Infusion of GG	1843:1856	Infusion of GG stimulated energy retention in body tissue, increased plasma glucose and insulin concentrations, decreased lipogenic metabolites in plasma, and decreased milk fat yield and milk energy output.
30527986	8	196	theme	LMP	1832:1834	arg1	level					1836:1840	the LMP level	1828:1840	the LMP level	1828:1840	Infusing GG or LG at the HMP level did not affect milk production differently than at the LMP level.
30527986	15	197	theme	HMP	2775:2777	arg1	level					2779:2783	the HMP level	2771:2783	the HMP level	2771:2783	Heat production increased at the HMP level, but only when GG and LG were infused.
30527986	12	198	theme	milk	2443:2446	arg1	efficiency					2450:2459	milk N efficiency	2443:2459	milk N efficiency	2443:2459	Infusion of LG decreased N intake but did not affect milk N efficiency or N retention.
30527986	11	199	theme	LG	2174:2175	arg1	Infusion					2162:2169	Infusion	2162:2169	Infusion of LG	2162:2175	Infusion of LG tended to increase metabolizable energy intake, increased milk fat yield and milk energy output, increased plasma triacylglycerides and long-chain fatty acid concentrations, and had no effect on energy retention.
30527986	4	200	theme	glucose	1026:1032	arg1	g/d					1019:1021	1,319 g/d	1013:1021	1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C)	1013:1179	Abomasal infusion treatments were saline (LMP-C), isoenergetic infusions (digestible energy basis) of 1,319 g/d of glucose (LMP-GG), 676 g/d of palm olein (LMP-LG; major fatty acid constituents are palmitic, oleic, and linoleic acid), or 844 g/d of essential AA (HMP-C), or isoenergetic infusions of 1,319 g/d of glucose + 844 g/d of essential AA (HMP-GG) or 676 g/d of palm olein + 844 g/d of essential AA (HMP-LG).
30527986	11	201	theme	long-chain	2313:2322	arg1	acid					2330:2333	long-chain fatty acid	2313:2333	long-chain fatty acid concentrations	2313:2348	Infusion of LG tended to increase metabolizable energy intake, increased milk fat yield and milk energy output, increased plasma triacylglycerides and long-chain fatty acid concentrations, and had no effect on energy retention.
30527986	5	202	theme	respiration	1368:1378	arg1	chambers					1380:1387	climate respiration chambers	1360:1387	climate respiration chambers	1360:1387	The experiment was conducted in climate respiration chambers to determine energy and N balance in conjunction with milk production and composition, nutrient digestibility, and plasma constituents.
30527986	14	203	theme	Milk	2638:2641	arg1	output					2650:2655	Milk energy output	2638:2655	Milk energy output	2638:2655	Milk energy output increased at the HMP level, and protein level did not affect total energy retention.
30527986	5	204	theme	nutrient	1476:1483	arg1	digestibility					1485:1497	nutrient digestibility	1476:1497	nutrient digestibility	1476:1497	The experiment was conducted in climate respiration chambers to determine energy and N balance in conjunction with milk production and composition, nutrient digestibility, and plasma constituents.
30527986	2	205	from	d	467:467	arg1	milk					472:475	milk	472:475	milk	472:475	Six rumen-fistulated, second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were randomly assigned to a 6 × 6 Latin square design in which each experimental period consisted of 5 d of continuous abomasal infusion followed by 2 d of rest.
30527986	3	206	theme	energy	791:796	arg1	requirements					824:835	metabolizable protein requirements	802:835	metabolizable protein requirements	802:835	A total mixed ration consisting of 42% corn silage, 31% grass silage, and 27% concentrate (dry matter basis) was formulated to meet 100 and 83% of net energy and metabolizable protein requirements, respectively, and was fed at 90% of ad libitum intake by individual cow.
30527986	3	206	theme	energy	791:796	arg1	%					782:782	100 and 83%	772:782	100 and 83% of net energy	772:796	A total mixed ration consisting of 42% corn silage, 31% grass silage, and 27% concentrate (dry matter basis) was formulated to meet 100 and 83% of net energy and metabolizable protein requirements, respectively, and was fed at 90% of ad libitum intake by individual cow.
30527986	3	206	theme	energy	791:796	arg1	energy					791:796	net energy	787:796	net energy	787:796	A total mixed ration consisting of 42% corn silage, 31% grass silage, and 27% concentrate (dry matter basis) was formulated to meet 100 and 83% of net energy and metabolizable protein requirements, respectively, and was fed at 90% of ad libitum intake by individual cow.
30527986	13	207	theme	energy	2573:2578	arg1	intake					2580:2585	gross and metabolizable energy intake	2549:2585	gross and metabolizable energy intake	2549:2585	Compared with the LMP level, the HMP level increased dry matter intake, gross and metabolizable energy intake, and total milk, fat, protein, and lactose yields.
30527986	17	208	theme	improved	3031:3038	arg1	efficiency					3047:3056	improved milk N efficiency	3031:3056	improved milk N efficiency	3031:3056	Regardless of protein level, GG promoted energy retention and improved milk N efficiency, but not through increased milk protein yield.
30527986	12	209	theme	LG	2402:2403	arg1	Infusion					2390:2397	Infusion	2390:2397	Infusion of LG	2390:2403	Infusion of LG decreased N intake but did not affect milk N efficiency or N retention.
31857157	4	0	theme	formulation	976:986	arg1	activity					953:960	the anti-tumor activity	938:960	the anti-tumor activity of an aqueous formulation of EcML	938:994	EcML is a mixture of 4'-monophosphoryl lipids A (MPLAs) produced directly by an engineered Escherichia coli strain; it has a unique congener composition that differentiates it from the well-established MPLA adjuvants, 3-O-desacyl-4'-monophosphoryl lipid A and glucopyranosyl lipid A. Given that active dendritic cells initiate adaptive immune responses, we investigated the anti-tumor activity of an aqueous formulation of EcML.
31857157	5	1	theme	naïve	1110:1114	arg1	cells					1118:1122	naïve T cells	1110:1122	naïve T cells	1110:1122	Upon sensing EcML via TLR-4, dendritic cells matured into powerful antigen-presenting cells that could stimulate naïve T cells.
31857157	2	2	theme	T	329:329	arg1	priming					336:342	impaired T cell priming	320:342	impaired T cell priming	320:342	In particular, low response rates to PD-1 blockades are highly correlated with impaired T cell priming.
31857157	1	3	theme	immune	222:227	arg1	conditions					229:238	the tumor's immune conditions	210:238	the tumor's immune conditions	210:238	Cancer immunotherapy is a powerful approach for cancer treatment, but its clinical effects rely on the tumor's immune conditions.
31857157	5	4	theme	T	1116:1116	arg1	cells					1118:1122	naïve T cells	1110:1122	naïve T cells	1110:1122	Upon sensing EcML via TLR-4, dendritic cells matured into powerful antigen-presenting cells that could stimulate naïve T cells.
31857157	6	5	theme	melanoma	1263:1270	arg1	model					1272:1276	the B16F10-OVA melanoma model	1248:1276	the B16F10-OVA melanoma model	1248:1276	EcML reduced tumor growth in the B16F10 mouse model via dendritic cell activation and potentiated PD-1 blockade therapy in the B16F10-OVA melanoma model.
31857157	2	6	theme	impaired	320:327	arg1	priming					336:342	impaired T cell priming	320:342	impaired T cell priming	320:342	In particular, low response rates to PD-1 blockades are highly correlated with impaired T cell priming.
31857157	6	7	theme	B16F10-OVA	1252:1261	arg1	model					1272:1276	the B16F10-OVA melanoma model	1248:1276	the B16F10-OVA melanoma model	1248:1276	EcML reduced tumor growth in the B16F10 mouse model via dendritic cell activation and potentiated PD-1 blockade therapy in the B16F10-OVA melanoma model.
31857157	2	8	theme	response	260:267	arg1	rates					269:273	low response rates	256:273	low response rates to PD-1 blockades	256:291	In particular, low response rates to PD-1 blockades are highly correlated with impaired T cell priming.
31857157	5	9	theme	powerful	1055:1062	arg1	cells					1083:1087	powerful antigen-presenting cells	1055:1087	powerful antigen-presenting cells that could stimulate naïve T cells	1055:1122	Upon sensing EcML via TLR-4, dendritic cells matured into powerful antigen-presenting cells that could stimulate naïve T cells.
31857157	7	10	theme	PD-1	1392:1395	arg1	therapy					1406:1412	PD-1 blockade therapy	1392:1412	PD-1 blockade therapy against tumors	1392:1427	These data identify EcML as a promising TLR-4 agonist that can induce anti-tumor immune responses and potentiate PD-1 blockade therapy against tumors.
31857157	5	11	theme	antigen-presenting	1064:1081	arg1	cells					1083:1087	powerful antigen-presenting cells	1055:1087	powerful antigen-presenting cells that could stimulate naïve T cells	1055:1122	Upon sensing EcML via TLR-4, dendritic cells matured into powerful antigen-presenting cells that could stimulate naïve T cells.
31857157	4	12	theme	unique	693:698	arg1	composition					709:719	a unique congener composition	691:719	a unique congener composition that differentiates it from the well-established MPLA adjuvants, 3-O-desacyl-4'-monophosphoryl lipid A and glucopyranosyl lipid A. Given that active dendritic cells initiate adaptive immune responses, we investigated the anti-tumor activity of an aqueous formulation of EcML	691:994	EcML is a mixture of 4'-monophosphoryl lipids A (MPLAs) produced directly by an engineered Escherichia coli strain; it has a unique congener composition that differentiates it from the well-established MPLA adjuvants, 3-O-desacyl-4'-monophosphoryl lipid A and glucopyranosyl lipid A. Given that active dendritic cells initiate adaptive immune responses, we investigated the anti-tumor activity of an aqueous formulation of EcML.
31857157	2	13	theme	low	256:258	arg1	rates					269:273	low response rates	256:273	low response rates to PD-1 blockades	256:291	In particular, low response rates to PD-1 blockades are highly correlated with impaired T cell priming.
31857157	4	14	theme	aqueous	968:974	arg1	formulation					976:986	an aqueous formulation	965:986	an aqueous formulation of EcML	965:994	EcML is a mixture of 4'-monophosphoryl lipids A (MPLAs) produced directly by an engineered Escherichia coli strain; it has a unique congener composition that differentiates it from the well-established MPLA adjuvants, 3-O-desacyl-4'-monophosphoryl lipid A and glucopyranosyl lipid A. Given that active dendritic cells initiate adaptive immune responses, we investigated the anti-tumor activity of an aqueous formulation of EcML.
31857157	6	15	from	therapy	1237:1243	arg1	model					1272:1276	the B16F10-OVA melanoma model	1248:1276	the B16F10-OVA melanoma model	1248:1276	EcML reduced tumor growth in the B16F10 mouse model via dendritic cell activation and potentiated PD-1 blockade therapy in the B16F10-OVA melanoma model.
31857157	0	16	theme	blockade	101:108	arg1	efficacy					84:91	the efficacy	80:91	the efficacy of PD-1 blockade	80:108	Dendritic cell activation by an E. coli-derived monophosphoryl lipid A enhances the efficacy of PD-1 blockade.
31857157	4	17	theme	lipid	816:820	arg1	A					822:822	3-O-desacyl-4'-monophosphoryl lipid A	786:822	3-O-desacyl-4'-monophosphoryl lipid A	786:822	EcML is a mixture of 4'-monophosphoryl lipids A (MPLAs) produced directly by an engineered Escherichia coli strain; it has a unique congener composition that differentiates it from the well-established MPLA adjuvants, 3-O-desacyl-4'-monophosphoryl lipid A and glucopyranosyl lipid A. Given that active dendritic cells initiate adaptive immune responses, we investigated the anti-tumor activity of an aqueous formulation of EcML.
31857157	4	17	theme	lipid	816:820	arg1	adjuvants					775:783	the well-established MPLA adjuvants	749:783	the well-established MPLA adjuvants	749:783	EcML is a mixture of 4'-monophosphoryl lipids A (MPLAs) produced directly by an engineered Escherichia coli strain; it has a unique congener composition that differentiates it from the well-established MPLA adjuvants, 3-O-desacyl-4'-monophosphoryl lipid A and glucopyranosyl lipid A. Given that active dendritic cells initiate adaptive immune responses, we investigated the anti-tumor activity of an aqueous formulation of EcML.
31857157	3	18	theme	dendritic	427:435	arg1	cells					437:441	dendritic cells	427:441	dendritic cells in a toll-like receptor-4 (TLR-4)-dependent manner	427:492	Here, we demonstrate that E. coli-derived monophosphoryl lipid A (EcML) activates dendritic cells in a toll-like receptor-4 (TLR-4)-dependent manner and increases the sensitivity of cancer cells to anti-PD-1 immunotherapy.
31857157	3	19	theme	-dependent	476:485	arg1	manner					487:492	a toll-like receptor-4 (TLR-4)-dependent manner	446:492	a toll-like receptor-4 (TLR-4)-dependent manner	446:492	Here, we demonstrate that E. coli-derived monophosphoryl lipid A (EcML) activates dendritic cells in a toll-like receptor-4 (TLR-4)-dependent manner and increases the sensitivity of cancer cells to anti-PD-1 immunotherapy.
31857157	6	20	from	growth	1144:1149	arg1	model					1171:1175	the B16F10 mouse model	1154:1175	the B16F10 mouse model	1154:1175	EcML reduced tumor growth in the B16F10 mouse model via dendritic cell activation and potentiated PD-1 blockade therapy in the B16F10-OVA melanoma model.
31857157	1	21	theme	cancer	159:164	arg1	treatment					166:174	cancer treatment	159:174	cancer treatment	159:174	Cancer immunotherapy is a powerful approach for cancer treatment, but its clinical effects rely on the tumor's immune conditions.
31857157	0	22	theme	PD-1	96:99	arg1	blockade					101:108	PD-1 blockade	96:108	PD-1 blockade	96:108	Dendritic cell activation by an E. coli-derived monophosphoryl lipid A enhances the efficacy of PD-1 blockade.
31857157	4	23	theme	3-O-desacyl-4'-monophosphoryl	786:814	arg1	A					822:822	3-O-desacyl-4'-monophosphoryl lipid A	786:822	3-O-desacyl-4'-monophosphoryl lipid A	786:822	EcML is a mixture of 4'-monophosphoryl lipids A (MPLAs) produced directly by an engineered Escherichia coli strain; it has a unique congener composition that differentiates it from the well-established MPLA adjuvants, 3-O-desacyl-4'-monophosphoryl lipid A and glucopyranosyl lipid A. Given that active dendritic cells initiate adaptive immune responses, we investigated the anti-tumor activity of an aqueous formulation of EcML.
31857157	4	23	theme	3-O-desacyl-4'-monophosphoryl	786:814	arg1	adjuvants					775:783	the well-established MPLA adjuvants	749:783	the well-established MPLA adjuvants	749:783	EcML is a mixture of 4'-monophosphoryl lipids A (MPLAs) produced directly by an engineered Escherichia coli strain; it has a unique congener composition that differentiates it from the well-established MPLA adjuvants, 3-O-desacyl-4'-monophosphoryl lipid A and glucopyranosyl lipid A. Given that active dendritic cells initiate adaptive immune responses, we investigated the anti-tumor activity of an aqueous formulation of EcML.
31857157	3	24	from	cells	437:441	arg1	manner					487:492	a toll-like receptor-4 (TLR-4)-dependent manner	446:492	a toll-like receptor-4 (TLR-4)-dependent manner	446:492	Here, we demonstrate that E. coli-derived monophosphoryl lipid A (EcML) activates dendritic cells in a toll-like receptor-4 (TLR-4)-dependent manner and increases the sensitivity of cancer cells to anti-PD-1 immunotherapy.
31857157	7	25	theme	promising	1309:1317	arg1	agonist					1325:1331	a promising TLR-4 agonist	1307:1331	a promising TLR-4 agonist that can induce anti-tumor immune responses and potentiate PD-1 blockade therapy against tumors	1307:1427	These data identify EcML as a promising TLR-4 agonist that can induce anti-tumor immune responses and potentiate PD-1 blockade therapy against tumors.
31857157	7	25	theme	promising	1309:1317	arg1	EcML					1299:1302	EcML	1299:1302	EcML	1299:1302	These data identify EcML as a promising TLR-4 agonist that can induce anti-tumor immune responses and potentiate PD-1 blockade therapy against tumors.
31857157	0	26	theme	cell	10:13	arg1	activation					15:24	Dendritic cell activation	0:24	Dendritic cell activation by an E. coli-derived monophosphoryl lipid A	0:69	Dendritic cell activation by an E. coli-derived monophosphoryl lipid A enhances the efficacy of PD-1 blockade.
31857157	6	27	theme	blockade	1228:1235	arg1	therapy					1237:1243	PD-1 blockade therapy	1223:1243	PD-1 blockade therapy in the B16F10-OVA melanoma model	1223:1276	EcML reduced tumor growth in the B16F10 mouse model via dendritic cell activation and potentiated PD-1 blockade therapy in the B16F10-OVA melanoma model.
31857157	0	28	link	coli-derived	35:46	arg1	A					69:69	an E. coli-derived monophosphoryl lipid A	29:69	an E. coli-derived monophosphoryl lipid A	29:69	Dendritic cell activation by an E. coli-derived monophosphoryl lipid A enhances the efficacy of PD-1 blockade.
31857157	6	29	theme	tumor	1138:1142	arg1	growth					1144:1149	tumor growth	1138:1149	tumor growth in the B16F10 mouse model	1138:1175	EcML reduced tumor growth in the B16F10 mouse model via dendritic cell activation and potentiated PD-1 blockade therapy in the B16F10-OVA melanoma model.
31857157	3	30	theme	monophosphoryl	387:400	arg1	A					408:408	E. coli-derived monophosphoryl lipid A	371:408	E. coli-derived monophosphoryl lipid A (EcML)	371:415	Here, we demonstrate that E. coli-derived monophosphoryl lipid A (EcML) activates dendritic cells in a toll-like receptor-4 (TLR-4)-dependent manner and increases the sensitivity of cancer cells to anti-PD-1 immunotherapy.
31857157	3	30	theme	monophosphoryl	387:400	arg1	EcML					411:414	EcML	411:414	EcML	411:414	Here, we demonstrate that E. coli-derived monophosphoryl lipid A (EcML) activates dendritic cells in a toll-like receptor-4 (TLR-4)-dependent manner and increases the sensitivity of cancer cells to anti-PD-1 immunotherapy.
31857157	0	31	theme	Dendritic	0:8	arg1	activation					15:24	Dendritic cell activation	0:24	Dendritic cell activation by an E. coli-derived monophosphoryl lipid A	0:69	Dendritic cell activation by an E. coli-derived monophosphoryl lipid A enhances the efficacy of PD-1 blockade.
31857157	4	32	contain	has	687:689	arg2	composition					709:719	a unique congener composition	691:719	a unique congener composition that differentiates it from the well-established MPLA adjuvants, 3-O-desacyl-4'-monophosphoryl lipid A and glucopyranosyl lipid A. Given that active dendritic cells initiate adaptive immune responses, we investigated the anti-tumor activity of an aqueous formulation of EcML	691:994	EcML is a mixture of 4'-monophosphoryl lipids A (MPLAs) produced directly by an engineered Escherichia coli strain; it has a unique congener composition that differentiates it from the well-established MPLA adjuvants, 3-O-desacyl-4'-monophosphoryl lipid A and glucopyranosyl lipid A. Given that active dendritic cells initiate adaptive immune responses, we investigated the anti-tumor activity of an aqueous formulation of EcML.
31857157	4	32	contain	has	687:689	arg1	it					684:685	it	684:685	it	684:685	EcML is a mixture of 4'-monophosphoryl lipids A (MPLAs) produced directly by an engineered Escherichia coli strain; it has a unique congener composition that differentiates it from the well-established MPLA adjuvants, 3-O-desacyl-4'-monophosphoryl lipid A and glucopyranosyl lipid A. Given that active dendritic cells initiate adaptive immune responses, we investigated the anti-tumor activity of an aqueous formulation of EcML.
31857157	6	33	theme	PD-1	1223:1226	arg1	therapy					1237:1243	PD-1 blockade therapy	1223:1243	PD-1 blockade therapy in the B16F10-OVA melanoma model	1223:1276	EcML reduced tumor growth in the B16F10 mouse model via dendritic cell activation and potentiated PD-1 blockade therapy in the B16F10-OVA melanoma model.
31857157	4	34	theme	EcML	991:994	arg1	formulation					976:986	an aqueous formulation	965:986	an aqueous formulation of EcML	965:994	EcML is a mixture of 4'-monophosphoryl lipids A (MPLAs) produced directly by an engineered Escherichia coli strain; it has a unique congener composition that differentiates it from the well-established MPLA adjuvants, 3-O-desacyl-4'-monophosphoryl lipid A and glucopyranosyl lipid A. Given that active dendritic cells initiate adaptive immune responses, we investigated the anti-tumor activity of an aqueous formulation of EcML.
31857157	2	35	theme	PD-1	278:281	arg1	blockades					283:291	PD-1 blockades	278:291	PD-1 blockades	278:291	In particular, low response rates to PD-1 blockades are highly correlated with impaired T cell priming.
31857157	3	36	theme	lipid	402:406	arg1	A					408:408	E. coli-derived monophosphoryl lipid A	371:408	E. coli-derived monophosphoryl lipid A (EcML)	371:415	Here, we demonstrate that E. coli-derived monophosphoryl lipid A (EcML) activates dendritic cells in a toll-like receptor-4 (TLR-4)-dependent manner and increases the sensitivity of cancer cells to anti-PD-1 immunotherapy.
31857157	3	36	theme	lipid	402:406	arg1	EcML					411:414	EcML	411:414	EcML	411:414	Here, we demonstrate that E. coli-derived monophosphoryl lipid A (EcML) activates dendritic cells in a toll-like receptor-4 (TLR-4)-dependent manner and increases the sensitivity of cancer cells to anti-PD-1 immunotherapy.
31857157	7	37	theme	blockade	1397:1404	arg1	therapy					1406:1412	PD-1 blockade therapy	1392:1412	PD-1 blockade therapy against tumors	1392:1427	These data identify EcML as a promising TLR-4 agonist that can induce anti-tumor immune responses and potentiate PD-1 blockade therapy against tumors.
31857157	4	38	theme	adaptive	895:902	arg1	responses					911:919	adaptive immune responses	895:919	adaptive immune responses	895:919	EcML is a mixture of 4'-monophosphoryl lipids A (MPLAs) produced directly by an engineered Escherichia coli strain; it has a unique congener composition that differentiates it from the well-established MPLA adjuvants, 3-O-desacyl-4'-monophosphoryl lipid A and glucopyranosyl lipid A. Given that active dendritic cells initiate adaptive immune responses, we investigated the anti-tumor activity of an aqueous formulation of EcML.
31857157	6	39	theme	cell	1191:1194	arg1	activation					1196:1205	dendritic cell activation	1181:1205	dendritic cell activation	1181:1205	EcML reduced tumor growth in the B16F10 mouse model via dendritic cell activation and potentiated PD-1 blockade therapy in the B16F10-OVA melanoma model.
31857157	4	40	dep	Escherichia	659:669	arg1	coli					671:674	coli	671:674	coli	671:674	EcML is a mixture of 4'-monophosphoryl lipids A (MPLAs) produced directly by an engineered Escherichia coli strain; it has a unique congener composition that differentiates it from the well-established MPLA adjuvants, 3-O-desacyl-4'-monophosphoryl lipid A and glucopyranosyl lipid A. Given that active dendritic cells initiate adaptive immune responses, we investigated the anti-tumor activity of an aqueous formulation of EcML.
31857157	4	41	theme	MPLA	770:773	arg1	A					822:822	3-O-desacyl-4'-monophosphoryl lipid A	786:822	3-O-desacyl-4'-monophosphoryl lipid A	786:822	EcML is a mixture of 4'-monophosphoryl lipids A (MPLAs) produced directly by an engineered Escherichia coli strain; it has a unique congener composition that differentiates it from the well-established MPLA adjuvants, 3-O-desacyl-4'-monophosphoryl lipid A and glucopyranosyl lipid A. Given that active dendritic cells initiate adaptive immune responses, we investigated the anti-tumor activity of an aqueous formulation of EcML.
31857157	4	41	theme	MPLA	770:773	arg1	A.					849:850	A.	849:850	A.	849:850	EcML is a mixture of 4'-monophosphoryl lipids A (MPLAs) produced directly by an engineered Escherichia coli strain; it has a unique congener composition that differentiates it from the well-established MPLA adjuvants, 3-O-desacyl-4'-monophosphoryl lipid A and glucopyranosyl lipid A. Given that active dendritic cells initiate adaptive immune responses, we investigated the anti-tumor activity of an aqueous formulation of EcML.
31857157	4	41	theme	MPLA	770:773	arg1	adjuvants					775:783	the well-established MPLA adjuvants	749:783	the well-established MPLA adjuvants	749:783	EcML is a mixture of 4'-monophosphoryl lipids A (MPLAs) produced directly by an engineered Escherichia coli strain; it has a unique congener composition that differentiates it from the well-established MPLA adjuvants, 3-O-desacyl-4'-monophosphoryl lipid A and glucopyranosyl lipid A. Given that active dendritic cells initiate adaptive immune responses, we investigated the anti-tumor activity of an aqueous formulation of EcML.
31857157	3	42	theme	cancer	527:532	arg1	cells					534:538	cancer cells	527:538	cancer cells	527:538	Here, we demonstrate that E. coli-derived monophosphoryl lipid A (EcML) activates dendritic cells in a toll-like receptor-4 (TLR-4)-dependent manner and increases the sensitivity of cancer cells to anti-PD-1 immunotherapy.
31857157	6	43	theme	B16F10	1158:1163	arg1	model					1171:1175	the B16F10 mouse model	1154:1175	the B16F10 mouse model	1154:1175	EcML reduced tumor growth in the B16F10 mouse model via dendritic cell activation and potentiated PD-1 blockade therapy in the B16F10-OVA melanoma model.
31857157	1	44	theme	clinical	185:192	arg1	effects					194:200	its clinical effects	181:200	its clinical effects	181:200	Cancer immunotherapy is a powerful approach for cancer treatment, but its clinical effects rely on the tumor's immune conditions.
31857157	3	45	theme	E.	371:372	arg1	A					408:408	E. coli-derived monophosphoryl lipid A	371:408	E. coli-derived monophosphoryl lipid A (EcML)	371:415	Here, we demonstrate that E. coli-derived monophosphoryl lipid A (EcML) activates dendritic cells in a toll-like receptor-4 (TLR-4)-dependent manner and increases the sensitivity of cancer cells to anti-PD-1 immunotherapy.
31857157	3	45	theme	E.	371:372	arg1	EcML					411:414	EcML	411:414	EcML	411:414	Here, we demonstrate that E. coli-derived monophosphoryl lipid A (EcML) activates dendritic cells in a toll-like receptor-4 (TLR-4)-dependent manner and increases the sensitivity of cancer cells to anti-PD-1 immunotherapy.
31857157	3	46	theme	toll-like	448:456	arg1	TLR-4					470:474	TLR-4	470:474	TLR-4	470:474	Here, we demonstrate that E. coli-derived monophosphoryl lipid A (EcML) activates dendritic cells in a toll-like receptor-4 (TLR-4)-dependent manner and increases the sensitivity of cancer cells to anti-PD-1 immunotherapy.
31857157	3	46	theme	toll-like	448:456	arg1	receptor-4					458:467	toll-like receptor-4	448:467	toll-like receptor-4 (TLR-4)	448:475	Here, we demonstrate that E. coli-derived monophosphoryl lipid A (EcML) activates dendritic cells in a toll-like receptor-4 (TLR-4)-dependent manner and increases the sensitivity of cancer cells to anti-PD-1 immunotherapy.
31857157	3	47	theme	anti-PD-1	543:551	arg1	immunotherapy					553:565	anti-PD-1 immunotherapy	543:565	anti-PD-1 immunotherapy	543:565	Here, we demonstrate that E. coli-derived monophosphoryl lipid A (EcML) activates dendritic cells in a toll-like receptor-4 (TLR-4)-dependent manner and increases the sensitivity of cancer cells to anti-PD-1 immunotherapy.
31857157	4	48	theme	lipids	607:612	arg1	EcML					568:571	EcML	568:571	EcML	568:571	EcML is a mixture of 4'-monophosphoryl lipids A (MPLAs) produced directly by an engineered Escherichia coli strain; it has a unique congener composition that differentiates it from the well-established MPLA adjuvants, 3-O-desacyl-4'-monophosphoryl lipid A and glucopyranosyl lipid A. Given that active dendritic cells initiate adaptive immune responses, we investigated the anti-tumor activity of an aqueous formulation of EcML.
31857157	4	48	theme	lipids	607:612	arg1	mixture					578:584	a mixture	576:584	a mixture of 4'-monophosphoryl lipids A (MPLAs) produced directly by an engineered Escherichia coli strain	576:681	EcML is a mixture of 4'-monophosphoryl lipids A (MPLAs) produced directly by an engineered Escherichia coli strain; it has a unique congener composition that differentiates it from the well-established MPLA adjuvants, 3-O-desacyl-4'-monophosphoryl lipid A and glucopyranosyl lipid A. Given that active dendritic cells initiate adaptive immune responses, we investigated the anti-tumor activity of an aqueous formulation of EcML.
31857157	3	49	theme	coli-derived	374:385	arg1	A					408:408	E. coli-derived monophosphoryl lipid A	371:408	E. coli-derived monophosphoryl lipid A (EcML)	371:415	Here, we demonstrate that E. coli-derived monophosphoryl lipid A (EcML) activates dendritic cells in a toll-like receptor-4 (TLR-4)-dependent manner and increases the sensitivity of cancer cells to anti-PD-1 immunotherapy.
31857157	3	49	theme	coli-derived	374:385	arg1	EcML					411:414	EcML	411:414	EcML	411:414	Here, we demonstrate that E. coli-derived monophosphoryl lipid A (EcML) activates dendritic cells in a toll-like receptor-4 (TLR-4)-dependent manner and increases the sensitivity of cancer cells to anti-PD-1 immunotherapy.
31857157	4	50	theme	immune	904:909	arg1	responses					911:919	adaptive immune responses	895:919	adaptive immune responses	895:919	EcML is a mixture of 4'-monophosphoryl lipids A (MPLAs) produced directly by an engineered Escherichia coli strain; it has a unique congener composition that differentiates it from the well-established MPLA adjuvants, 3-O-desacyl-4'-monophosphoryl lipid A and glucopyranosyl lipid A. Given that active dendritic cells initiate adaptive immune responses, we investigated the anti-tumor activity of an aqueous formulation of EcML.
31857157	4	51	dep	lipids	607:612	arg1	MPLAs					617:621	MPLAs	617:621	MPLAs	617:621	EcML is a mixture of 4'-monophosphoryl lipids A (MPLAs) produced directly by an engineered Escherichia coli strain; it has a unique congener composition that differentiates it from the well-established MPLA adjuvants, 3-O-desacyl-4'-monophosphoryl lipid A and glucopyranosyl lipid A. Given that active dendritic cells initiate adaptive immune responses, we investigated the anti-tumor activity of an aqueous formulation of EcML.
31857157	4	51	dep	lipids	607:612	arg1	A					614:614	A	614:614	4'-monophosphoryl lipids A (MPLAs) produced directly by an engineered Escherichia coli strain	589:681	EcML is a mixture of 4'-monophosphoryl lipids A (MPLAs) produced directly by an engineered Escherichia coli strain; it has a unique congener composition that differentiates it from the well-established MPLA adjuvants, 3-O-desacyl-4'-monophosphoryl lipid A and glucopyranosyl lipid A. Given that active dendritic cells initiate adaptive immune responses, we investigated the anti-tumor activity of an aqueous formulation of EcML.
31857157	0	52	dep	coli-derived	35:46	arg1	E.					32:33	E.	32:33	E.	32:33	Dendritic cell activation by an E. coli-derived monophosphoryl lipid A enhances the efficacy of PD-1 blockade.
31857157	7	53	theme	anti-tumor	1349:1358	arg1	responses					1367:1375	anti-tumor immune responses	1349:1375	anti-tumor immune responses	1349:1375	These data identify EcML as a promising TLR-4 agonist that can induce anti-tumor immune responses and potentiate PD-1 blockade therapy against tumors.
31857157	4	54	theme	4'-monophosphoryl	589:605	arg1	lipids					607:612	4'-monophosphoryl lipids A (MPLAs)	589:622	4'-monophosphoryl lipids A (MPLAs) produced directly by an engineered Escherichia coli strain	589:681	EcML is a mixture of 4'-monophosphoryl lipids A (MPLAs) produced directly by an engineered Escherichia coli strain; it has a unique congener composition that differentiates it from the well-established MPLA adjuvants, 3-O-desacyl-4'-monophosphoryl lipid A and glucopyranosyl lipid A. Given that active dendritic cells initiate adaptive immune responses, we investigated the anti-tumor activity of an aqueous formulation of EcML.
31857157	0	55	theme	monophosphoryl	48:61	arg1	A					69:69	an E. coli-derived monophosphoryl lipid A	29:69	an E. coli-derived monophosphoryl lipid A	29:69	Dendritic cell activation by an E. coli-derived monophosphoryl lipid A enhances the efficacy of PD-1 blockade.
31857157	4	56	theme	active	863:868	arg1	cells					880:884	active dendritic cells	863:884	active dendritic cells	863:884	EcML is a mixture of 4'-monophosphoryl lipids A (MPLAs) produced directly by an engineered Escherichia coli strain; it has a unique congener composition that differentiates it from the well-established MPLA adjuvants, 3-O-desacyl-4'-monophosphoryl lipid A and glucopyranosyl lipid A. Given that active dendritic cells initiate adaptive immune responses, we investigated the anti-tumor activity of an aqueous formulation of EcML.
31857157	4	57	theme	anti-tumor	942:951	arg1	activity					953:960	the anti-tumor activity	938:960	the anti-tumor activity of an aqueous formulation of EcML	938:994	EcML is a mixture of 4'-monophosphoryl lipids A (MPLAs) produced directly by an engineered Escherichia coli strain; it has a unique congener composition that differentiates it from the well-established MPLA adjuvants, 3-O-desacyl-4'-monophosphoryl lipid A and glucopyranosyl lipid A. Given that active dendritic cells initiate adaptive immune responses, we investigated the anti-tumor activity of an aqueous formulation of EcML.
31857157	3	58	theme	cells	534:538	arg1	sensitivity					512:522	the sensitivity	508:522	the sensitivity of cancer cells to anti-PD-1 immunotherapy	508:565	Here, we demonstrate that E. coli-derived monophosphoryl lipid A (EcML) activates dendritic cells in a toll-like receptor-4 (TLR-4)-dependent manner and increases the sensitivity of cancer cells to anti-PD-1 immunotherapy.
31857157	4	59	theme	Escherichia	659:669	arg1	strain					676:681	an engineered Escherichia coli strain	645:681	an engineered Escherichia coli strain	645:681	EcML is a mixture of 4'-monophosphoryl lipids A (MPLAs) produced directly by an engineered Escherichia coli strain; it has a unique congener composition that differentiates it from the well-established MPLA adjuvants, 3-O-desacyl-4'-monophosphoryl lipid A and glucopyranosyl lipid A. Given that active dendritic cells initiate adaptive immune responses, we investigated the anti-tumor activity of an aqueous formulation of EcML.
31857157	0	60	theme	coli-derived	35:46	arg1	A					69:69	an E. coli-derived monophosphoryl lipid A	29:69	an E. coli-derived monophosphoryl lipid A	29:69	Dendritic cell activation by an E. coli-derived monophosphoryl lipid A enhances the efficacy of PD-1 blockade.
31857157	7	61	theme	immune	1360:1365	arg1	responses					1367:1375	anti-tumor immune responses	1349:1375	anti-tumor immune responses	1349:1375	These data identify EcML as a promising TLR-4 agonist that can induce anti-tumor immune responses and potentiate PD-1 blockade therapy against tumors.
31857157	3	62	link	coli-derived	374:385	arg1	A					408:408	E. coli-derived monophosphoryl lipid A	371:408	E. coli-derived monophosphoryl lipid A (EcML)	371:415	Here, we demonstrate that E. coli-derived monophosphoryl lipid A (EcML) activates dendritic cells in a toll-like receptor-4 (TLR-4)-dependent manner and increases the sensitivity of cancer cells to anti-PD-1 immunotherapy.
31857157	3	62	link	coli-derived	374:385	arg1	EcML					411:414	EcML	411:414	EcML	411:414	Here, we demonstrate that E. coli-derived monophosphoryl lipid A (EcML) activates dendritic cells in a toll-like receptor-4 (TLR-4)-dependent manner and increases the sensitivity of cancer cells to anti-PD-1 immunotherapy.
31857157	1	63	theme	Cancer	111:116	arg1	immunotherapy					118:130	Cancer immunotherapy	111:130	Cancer immunotherapy	111:130	Cancer immunotherapy is a powerful approach for cancer treatment, but its clinical effects rely on the tumor's immune conditions.
31857157	1	63	theme	Cancer	111:116	arg1	approach					146:153	a powerful approach	135:153	a powerful approach for cancer treatment	135:174	Cancer immunotherapy is a powerful approach for cancer treatment, but its clinical effects rely on the tumor's immune conditions.
31857157	4	64	dep	A.	849:850	arg1	investigated					925:936	investigated	925:936	investigated the anti-tumor activity of an aqueous formulation of EcML	925:994	EcML is a mixture of 4'-monophosphoryl lipids A (MPLAs) produced directly by an engineered Escherichia coli strain; it has a unique congener composition that differentiates it from the well-established MPLA adjuvants, 3-O-desacyl-4'-monophosphoryl lipid A and glucopyranosyl lipid A. Given that active dendritic cells initiate adaptive immune responses, we investigated the anti-tumor activity of an aqueous formulation of EcML.
31857157	6	65	theme	dendritic	1181:1189	arg1	activation					1196:1205	dendritic cell activation	1181:1205	dendritic cell activation	1181:1205	EcML reduced tumor growth in the B16F10 mouse model via dendritic cell activation and potentiated PD-1 blockade therapy in the B16F10-OVA melanoma model.
31857157	4	66	theme	well-established	753:768	arg1	A					822:822	3-O-desacyl-4'-monophosphoryl lipid A	786:822	3-O-desacyl-4'-monophosphoryl lipid A	786:822	EcML is a mixture of 4'-monophosphoryl lipids A (MPLAs) produced directly by an engineered Escherichia coli strain; it has a unique congener composition that differentiates it from the well-established MPLA adjuvants, 3-O-desacyl-4'-monophosphoryl lipid A and glucopyranosyl lipid A. Given that active dendritic cells initiate adaptive immune responses, we investigated the anti-tumor activity of an aqueous formulation of EcML.
31857157	4	66	theme	well-established	753:768	arg1	A.					849:850	A.	849:850	A.	849:850	EcML is a mixture of 4'-monophosphoryl lipids A (MPLAs) produced directly by an engineered Escherichia coli strain; it has a unique congener composition that differentiates it from the well-established MPLA adjuvants, 3-O-desacyl-4'-monophosphoryl lipid A and glucopyranosyl lipid A. Given that active dendritic cells initiate adaptive immune responses, we investigated the anti-tumor activity of an aqueous formulation of EcML.
31857157	4	66	theme	well-established	753:768	arg1	adjuvants					775:783	the well-established MPLA adjuvants	749:783	the well-established MPLA adjuvants	749:783	EcML is a mixture of 4'-monophosphoryl lipids A (MPLAs) produced directly by an engineered Escherichia coli strain; it has a unique congener composition that differentiates it from the well-established MPLA adjuvants, 3-O-desacyl-4'-monophosphoryl lipid A and glucopyranosyl lipid A. Given that active dendritic cells initiate adaptive immune responses, we investigated the anti-tumor activity of an aqueous formulation of EcML.
31857157	4	67	theme	dendritic	870:878	arg1	cells					880:884	active dendritic cells	863:884	active dendritic cells	863:884	EcML is a mixture of 4'-monophosphoryl lipids A (MPLAs) produced directly by an engineered Escherichia coli strain; it has a unique congener composition that differentiates it from the well-established MPLA adjuvants, 3-O-desacyl-4'-monophosphoryl lipid A and glucopyranosyl lipid A. Given that active dendritic cells initiate adaptive immune responses, we investigated the anti-tumor activity of an aqueous formulation of EcML.
31857157	4	68	theme	congener	700:707	arg1	composition					709:719	a unique congener composition	691:719	a unique congener composition that differentiates it from the well-established MPLA adjuvants, 3-O-desacyl-4'-monophosphoryl lipid A and glucopyranosyl lipid A. Given that active dendritic cells initiate adaptive immune responses, we investigated the anti-tumor activity of an aqueous formulation of EcML	691:994	EcML is a mixture of 4'-monophosphoryl lipids A (MPLAs) produced directly by an engineered Escherichia coli strain; it has a unique congener composition that differentiates it from the well-established MPLA adjuvants, 3-O-desacyl-4'-monophosphoryl lipid A and glucopyranosyl lipid A. Given that active dendritic cells initiate adaptive immune responses, we investigated the anti-tumor activity of an aqueous formulation of EcML.
31857157	1	69	theme	powerful	137:144	arg1	immunotherapy					118:130	Cancer immunotherapy	111:130	Cancer immunotherapy	111:130	Cancer immunotherapy is a powerful approach for cancer treatment, but its clinical effects rely on the tumor's immune conditions.
31857157	1	69	theme	powerful	137:144	arg1	approach					146:153	a powerful approach	135:153	a powerful approach for cancer treatment	135:174	Cancer immunotherapy is a powerful approach for cancer treatment, but its clinical effects rely on the tumor's immune conditions.
31857157	5	70	theme	dendritic	1026:1034	arg1	cells					1036:1040	dendritic cells	1026:1040	dendritic cells	1026:1040	Upon sensing EcML via TLR-4, dendritic cells matured into powerful antigen-presenting cells that could stimulate naïve T cells.
31857157	7	71	theme	TLR-4	1319:1323	arg1	agonist					1325:1331	a promising TLR-4 agonist	1307:1331	a promising TLR-4 agonist that can induce anti-tumor immune responses and potentiate PD-1 blockade therapy against tumors	1307:1427	These data identify EcML as a promising TLR-4 agonist that can induce anti-tumor immune responses and potentiate PD-1 blockade therapy against tumors.
31857157	7	71	theme	TLR-4	1319:1323	arg1	EcML					1299:1302	EcML	1299:1302	EcML	1299:1302	These data identify EcML as a promising TLR-4 agonist that can induce anti-tumor immune responses and potentiate PD-1 blockade therapy against tumors.
31857157	0	72	theme	lipid	63:67	arg1	A					69:69	an E. coli-derived monophosphoryl lipid A	29:69	an E. coli-derived monophosphoryl lipid A	29:69	Dendritic cell activation by an E. coli-derived monophosphoryl lipid A enhances the efficacy of PD-1 blockade.
31857157	6	73	theme	mouse	1165:1169	arg1	model					1171:1175	the B16F10 mouse model	1154:1175	the B16F10 mouse model	1154:1175	EcML reduced tumor growth in the B16F10 mouse model via dendritic cell activation and potentiated PD-1 blockade therapy in the B16F10-OVA melanoma model.
31857157	2	74	theme	cell	331:334	arg1	priming					336:342	impaired T cell priming	320:342	impaired T cell priming	320:342	In particular, low response rates to PD-1 blockades are highly correlated with impaired T cell priming.
31857157	4	75	theme	engineered	648:657	arg1	strain					676:681	an engineered Escherichia coli strain	645:681	an engineered Escherichia coli strain	645:681	EcML is a mixture of 4'-monophosphoryl lipids A (MPLAs) produced directly by an engineered Escherichia coli strain; it has a unique congener composition that differentiates it from the well-established MPLA adjuvants, 3-O-desacyl-4'-monophosphoryl lipid A and glucopyranosyl lipid A. Given that active dendritic cells initiate adaptive immune responses, we investigated the anti-tumor activity of an aqueous formulation of EcML.
30576771	4	0	theme	paw	953:955	arg1	edema					957:961	carrageenan induced rat paw edema	929:961	carrageenan induced rat paw edema	929:961	The anti-inflammatory property of the essential was established by measurement of carrageenan induced rat paw edema while the anti-nociceptive activity was determined by hot plate test, according to established procedures.
30576771	8	1	theme	standard	1554:1561	arg1	drug					1563:1566	the standard drug	1550:1566	the standard drug	1550:1566	The anti-nociceptive property of the essential oil was statistically significant p < 0.001 at all doses of when compared to the control while exhibiting an activity in tandem with the standard drug.
30576771	2	2	theme	pharmacological	376:390	arg1	activity					392:399	pharmacological activity	376:399	pharmacological activity of B. glabra	376:412	AIM OF THE STUDY Considering the lack of scientific studies focused on chemical constituents and pharmacological activity of B. glabra essential oil, this work was designed to characterize the volatile compounds and evaluate the anti-inflammatory and anti-nociceptive properties ascribed to this plant species.
30576771	4	3	theme	induced	941:947	arg1	edema					957:961	carrageenan induced rat paw edema	929:961	carrageenan induced rat paw edema	929:961	The anti-inflammatory property of the essential was established by measurement of carrageenan induced rat paw edema while the anti-nociceptive activity was determined by hot plate test, according to established procedures.
30576771	9	4	theme	200 mg	1763:1768	arg1	dose					1755:1758	a dose	1753:1758	a dose of 200 mg	1753:1768	For the 1st and 2nd h, at doses of 100 and 200 mg/kg, the anti-inflammatory activity was statistically very significant (p < 0.001), while at 3rd h the activity declined (p < 0.01) at a dose of 200 mg.
30576771	3	5	from	hydrodistillation	674:690	arg1	apparatus					723:731	an all glass Clevenger-type apparatus	695:731	an all glass Clevenger-type apparatus	695:731	MATERIALS AND METHODS The essential oil was extracted from the leaf of B. glabra by hydrodistillation in an all glass Clevenger-type apparatus and characterized by gas chromatography (GC-FID) and gas chromatography-mass spectrometry (GC-MS) on HP-5 column.
30576771	10	6	theme	4th	1811:1813	arg1	duration					1830:1837	the 4th h experimental duration	1807:1837	the 4th h experimental duration	1807:1837	The activity was non-significant at the 4th h experimental duration.
30576771	11	7	theme	chemical	1880:1887	arg1	constituents					1889:1900	the chemical constituents	1876:1900	the chemical constituents	1876:1900	CONCLUSIONS This is first report on the chemical constituents and biological activity of essential oil of B. glabra from Nigeria.
30576771	9	8	theme	2nd	1585:1587	arg1	h					1589:1589	2nd h	1585:1589	2nd h	1585:1589	For the 1st and 2nd h, at doses of 100 and 200 mg/kg, the anti-inflammatory activity was statistically very significant (p < 0.001), while at 3rd h the activity declined (p < 0.01) at a dose of 200 mg.
30576771	9	9	dep	declined	1730:1737	arg1	p < 0.01					1740:1747	p < 0.01	1740:1747	p < 0.01	1740:1747	For the 1st and 2nd h, at doses of 100 and 200 mg/kg, the anti-inflammatory activity was statistically very significant (p < 0.001), while at 3rd h the activity declined (p < 0.01) at a dose of 200 mg.
30576771	2	10	theme	STUDY	290:294	arg1	AIM					279:281	AIM	279:281	AIM OF THE STUDY	279:294	AIM OF THE STUDY Considering the lack of scientific studies focused on chemical constituents and pharmacological activity of B. glabra essential oil, this work was designed to characterize the volatile compounds and evaluate the anti-inflammatory and anti-nociceptive properties ascribed to this plant species.
30576771	4	11	theme	rat	949:951	arg1	edema					957:961	carrageenan induced rat paw edema	929:961	carrageenan induced rat paw edema	929:961	The anti-inflammatory property of the essential was established by measurement of carrageenan induced rat paw edema while the anti-nociceptive activity was determined by hot plate test, according to established procedures.
30576771	3	12	theme	chromatography-mass	790:808	arg1	GC-MS					824:828	GC-MS	824:828	GC-MS	824:828	MATERIALS AND METHODS The essential oil was extracted from the leaf of B. glabra by hydrodistillation in an all glass Clevenger-type apparatus and characterized by gas chromatography (GC-FID) and gas chromatography-mass spectrometry (GC-MS) on HP-5 column.
30576771	3	12	theme	chromatography-mass	790:808	arg1	spectrometry					810:821	gas chromatography-mass spectrometry	786:821	gas chromatography-mass spectrometry (GC-MS)	786:829	MATERIALS AND METHODS The essential oil was extracted from the leaf of B. glabra by hydrodistillation in an all glass Clevenger-type apparatus and characterized by gas chromatography (GC-FID) and gas chromatography-mass spectrometry (GC-MS) on HP-5 column.
30576771	2	13	theme	studies	331:337	arg1	activity					392:399	pharmacological activity	376:399	pharmacological activity of B. glabra	376:412	AIM OF THE STUDY Considering the lack of scientific studies focused on chemical constituents and pharmacological activity of B. glabra essential oil, this work was designed to characterize the volatile compounds and evaluate the anti-inflammatory and anti-nociceptive properties ascribed to this plant species.
30576771	2	13	theme	studies	331:337	arg1	lack					312:315	the lack	308:315	the lack of scientific studies focused on chemical constituents	308:370	AIM OF THE STUDY Considering the lack of scientific studies focused on chemical constituents and pharmacological activity of B. glabra essential oil, this work was designed to characterize the volatile compounds and evaluate the anti-inflammatory and anti-nociceptive properties ascribed to this plant species.
30576771	1	14	theme	inflammatory-related	213:232	arg1	conditions					234:243	inflammatory-related conditions	213:243	inflammatory-related conditions	213:243	ETHNOPHARMACOLOGICAL RELEVANCE Bougainvillea glabra is widely used in Nigeria for the treatment of inflammatory-related conditions, and as remedy for pain ailments.
30576771	4	15	theme	anti-nociceptive	973:988	arg1	activity					990:997	the anti-nociceptive activity	969:997	the anti-nociceptive activity	969:997	The anti-inflammatory property of the essential was established by measurement of carrageenan induced rat paw edema while the anti-nociceptive activity was determined by hot plate test, according to established procedures.
30576771	10	16	theme	experimental	1817:1828	arg1	duration					1830:1837	the 4th h experimental duration	1807:1837	the 4th h experimental duration	1807:1837	The activity was non-significant at the 4th h experimental duration.
30576771	8	17	with	tandem	1538:1543	arg1	drug					1563:1566	the standard drug	1550:1566	the standard drug	1550:1566	The anti-nociceptive property of the essential oil was statistically significant p < 0.001 at all doses of when compared to the control while exhibiting an activity in tandem with the standard drug.
30576771	9	18	theme	100	1604:1606	arg1	doses					1595:1599	doses	1595:1599	doses of 100 and 200 mg/kg	1595:1620	For the 1st and 2nd h, at doses of 100 and 200 mg/kg, the anti-inflammatory activity was statistically very significant (p < 0.001), while at 3rd h the activity declined (p < 0.01) at a dose of 200 mg.
30576771	8	19	from	activity	1526:1533	arg1	tandem					1538:1543	tandem	1538:1543	tandem	1538:1543	The anti-nociceptive property of the essential oil was statistically significant p < 0.001 at all doses of when compared to the control while exhibiting an activity in tandem with the standard drug.
30576771	5	20	theme	%	1127:1127	arg1	yield					1114:1118	a yield	1112:1118	a yield of 0.08% (v/w) calculated on dry weight basis	1112:1164	RESULTS The essential oil was obtained in a yield of 0.08% (v/w) calculated on dry weight basis.
30576771	9	21	theme	200 mg/kg	1612:1620	arg1	doses					1595:1599	doses	1595:1599	doses of 100 and 200 mg/kg	1595:1620	For the 1st and 2nd h, at doses of 100 and 200 mg/kg, the anti-inflammatory activity was statistically very significant (p < 0.001), while at 3rd h the activity declined (p < 0.01) at a dose of 200 mg.
30576771	8	22	theme	anti-nociceptive	1374:1389	arg1	p < 0.001					1451:1459	statistically significant p < 0.001	1425:1459	statistically significant p < 0.001	1425:1459	The anti-nociceptive property of the essential oil was statistically significant p < 0.001 at all doses of when compared to the control while exhibiting an activity in tandem with the standard drug.
30576771	8	22	theme	anti-nociceptive	1374:1389	arg1	property					1391:1398	The anti-nociceptive property	1370:1398	The anti-nociceptive property of the essential oil	1370:1419	The anti-nociceptive property of the essential oil was statistically significant p < 0.001 at all doses of when compared to the control while exhibiting an activity in tandem with the standard drug.
30576771	6	23	theme	contents	1221:1228	arg1	contents					1221:1228	the oil contents	1213:1228	the oil contents	1213:1228	A total of 11 compounds representing 96.2% of the oil contents were identified by GC/MS.
30576771	6	23	theme	contents	1221:1228	arg1	%					1208:1208	96.2%	1204:1208	96.2% of the oil contents	1204:1228	A total of 11 compounds representing 96.2% of the oil contents were identified by GC/MS.
30576771	3	24	theme	glabra	664:669	arg1	leaf					653:656	the leaf	649:656	the leaf of B. glabra	649:669	MATERIALS AND METHODS The essential oil was extracted from the leaf of B. glabra by hydrodistillation in an all glass Clevenger-type apparatus and characterized by gas chromatography (GC-FID) and gas chromatography-mass spectrometry (GC-MS) on HP-5 column.
30576771	8	25	theme	essential	1407:1415	arg1	oil					1417:1419	the essential oil	1403:1419	the essential oil	1403:1419	The anti-nociceptive property of the essential oil was statistically significant p < 0.001 at all doses of when compared to the control while exhibiting an activity in tandem with the standard drug.
30576771	0	26	theme	essential	75:83	arg1	oil					85:87	essential oil	75:87	essential oil of Bougainvillea glabra	75:111	Chemical composition, anti-nociceptive and anti-inflammatory activities of essential oil of Bougainvillea glabra.
30576771	5	27	theme	dry	1149:1151	arg1	basis					1160:1164	dry weight basis	1149:1164	dry weight basis	1149:1164	RESULTS The essential oil was obtained in a yield of 0.08% (v/w) calculated on dry weight basis.
30576771	9	28	theme	anti-inflammatory	1627:1643	arg1	activity					1645:1652	the anti-inflammatory activity	1623:1652	the anti-inflammatory activity	1623:1652	For the 1st and 2nd h, at doses of 100 and 200 mg/kg, the anti-inflammatory activity was statistically very significant (p < 0.001), while at 3rd h the activity declined (p < 0.01) at a dose of 200 mg.
30576771	9	28	theme	anti-inflammatory	1627:1643	arg1	p < 0.001					1690:1698	p < 0.001	1690:1698	p < 0.001	1690:1698	For the 1st and 2nd h, at doses of 100 and 200 mg/kg, the anti-inflammatory activity was statistically very significant (p < 0.001), while at 3rd h the activity declined (p < 0.01) at a dose of 200 mg.
30576771	9	28	theme	anti-inflammatory	1627:1643	arg1	significant					1677:1687	significant	1677:1687	significant	1677:1687	For the 1st and 2nd h, at doses of 100 and 200 mg/kg, the anti-inflammatory activity was statistically very significant (p < 0.001), while at 3rd h the activity declined (p < 0.01) at a dose of 200 mg.
30576771	2	29	dep	lack	312:315	arg1	oil					424:426	essential oil	414:426	essential oil	414:426	AIM OF THE STUDY Considering the lack of scientific studies focused on chemical constituents and pharmacological activity of B. glabra essential oil, this work was designed to characterize the volatile compounds and evaluate the anti-inflammatory and anti-nociceptive properties ascribed to this plant species.
30576771	2	30	theme	plant	575:579	arg1	species					581:587	this plant species	570:587	this plant species	570:587	AIM OF THE STUDY Considering the lack of scientific studies focused on chemical constituents and pharmacological activity of B. glabra essential oil, this work was designed to characterize the volatile compounds and evaluate the anti-inflammatory and anti-nociceptive properties ascribed to this plant species.
30576771	7	31	theme	E	1328:1328	arg1	-β-ionone					1330:1338	(E)-β-ionone	1327:1338	(E)-β-ionone (10.3%)	1327:1346	The main constituents of the essential oil were (E)-nerolidol (31.4%), (E)-β-ionone (10.3%) and linalool (10.1%).
30576771	7	31	theme	E	1328:1328	arg1	%					1345:1345	10.3%	1341:1345	10.3%	1341:1345	The main constituents of the essential oil were (E)-nerolidol (31.4%), (E)-β-ionone (10.3%) and linalool (10.1%).
30576771	0	32	theme	glabra	106:111	arg1	oil					85:87	essential oil	75:87	essential oil of Bougainvillea glabra	75:111	Chemical composition, anti-nociceptive and anti-inflammatory activities of essential oil of Bougainvillea glabra.
30576771	10	33	from	duration	1830:1837	arg1	activity					1775:1782	The activity	1771:1782	The activity	1771:1782	The activity was non-significant at the 4th h experimental duration.
30576771	10	33	from	duration	1830:1837	arg1	non-significant					1788:1802	non-significant	1788:1802	non-significant	1788:1802	The activity was non-significant at the 4th h experimental duration.
30576771	4	34	theme	plate	1021:1025	arg1	test					1027:1030	hot plate test	1017:1030	hot plate test	1017:1030	The anti-inflammatory property of the essential was established by measurement of carrageenan induced rat paw edema while the anti-nociceptive activity was determined by hot plate test, according to established procedures.
30576771	1	35	dep	Bougainvillea	145:157	arg1	glabra					159:164	glabra	159:164	glabra	159:164	ETHNOPHARMACOLOGICAL RELEVANCE Bougainvillea glabra is widely used in Nigeria for the treatment of inflammatory-related conditions, and as remedy for pain ailments.
30576771	4	36	dep	induced	941:947	arg1	carrageenan					929:939	carrageenan	929:939	carrageenan	929:939	The anti-inflammatory property of the essential was established by measurement of carrageenan induced rat paw edema while the anti-nociceptive activity was determined by hot plate test, according to established procedures.
30576771	2	37	theme	volatile	472:479	arg1	compounds					481:489	the volatile compounds	468:489	the volatile compounds	468:489	AIM OF THE STUDY Considering the lack of scientific studies focused on chemical constituents and pharmacological activity of B. glabra essential oil, this work was designed to characterize the volatile compounds and evaluate the anti-inflammatory and anti-nociceptive properties ascribed to this plant species.
30576771	2	38	theme	essential	414:422	arg1	oil					424:426	essential oil	414:426	essential oil	414:426	AIM OF THE STUDY Considering the lack of scientific studies focused on chemical constituents and pharmacological activity of B. glabra essential oil, this work was designed to characterize the volatile compounds and evaluate the anti-inflammatory and anti-nociceptive properties ascribed to this plant species.
30576771	0	39	theme	Chemical	0:7	arg1	composition					9:19	Chemical composition	0:19	Chemical composition	0:19	Chemical composition, anti-nociceptive and anti-inflammatory activities of essential oil of Bougainvillea glabra.
30576771	12	40	theme	anti-inflammatory	2056:2072	arg1	properties					2074:2083	the anti-nociceptive and anti-inflammatory properties	2031:2083	the anti-nociceptive and anti-inflammatory properties traditionally ascribed to B. glabra	2031:2119	Overall, the results herein presented sustain and strengthen the anti-nociceptive and anti-inflammatory properties traditionally ascribed to B. glabra.
30576771	8	41	from	doses	1468:1472	arg1	property					1391:1398	The anti-nociceptive property	1370:1398	The anti-nociceptive property of the essential oil	1370:1419	The anti-nociceptive property of the essential oil was statistically significant p < 0.001 at all doses of when compared to the control while exhibiting an activity in tandem with the standard drug.
30576771	8	41	from	doses	1468:1472	arg1	p < 0.001					1451:1459	statistically significant p < 0.001	1425:1459	statistically significant p < 0.001	1425:1459	The anti-nociceptive property of the essential oil was statistically significant p < 0.001 at all doses of when compared to the control while exhibiting an activity in tandem with the standard drug.
30576771	5	42	theme	essential	1082:1090	arg1	oil					1092:1094	The essential oil	1078:1094	The essential oil	1078:1094	RESULTS The essential oil was obtained in a yield of 0.08% (v/w) calculated on dry weight basis.
30576771	7	43	theme	oil	1295:1297	arg1	constituents					1265:1276	The main constituents	1256:1276	The main constituents of the essential oil	1256:1297	The main constituents of the essential oil were (E)-nerolidol (31.4%), (E)-β-ionone (10.3%) and linalool (10.1%).
30576771	7	43	theme	oil	1295:1297	arg1	-nerolidol					1307:1316	(E)-nerolidol	1304:1316	(E)-nerolidol (31.4%)	1304:1324	The main constituents of the essential oil were (E)-nerolidol (31.4%), (E)-β-ionone (10.3%) and linalool (10.1%).
30576771	2	44	theme	chemical	350:357	arg1	constituents					359:370	chemical constituents	350:370	chemical constituents	350:370	AIM OF THE STUDY Considering the lack of scientific studies focused on chemical constituents and pharmacological activity of B. glabra essential oil, this work was designed to characterize the volatile compounds and evaluate the anti-inflammatory and anti-nociceptive properties ascribed to this plant species.
30576771	1	45	used	used	176:179	arg2	RELEVANCE					135:143	ETHNOPHARMACOLOGICAL RELEVANCE	114:143	ETHNOPHARMACOLOGICAL RELEVANCE Bougainvillea glabra	114:164	ETHNOPHARMACOLOGICAL RELEVANCE Bougainvillea glabra is widely used in Nigeria for the treatment of inflammatory-related conditions, and as remedy for pain ailments.
30576771	1	45	used	used	176:179	arg2	remedy					253:258	remedy	253:258	remedy	253:258	ETHNOPHARMACOLOGICAL RELEVANCE Bougainvillea glabra is widely used in Nigeria for the treatment of inflammatory-related conditions, and as remedy for pain ailments.
30576771	3	46	theme	glass	702:706	arg1	apparatus					723:731	an all glass Clevenger-type apparatus	695:731	an all glass Clevenger-type apparatus	695:731	MATERIALS AND METHODS The essential oil was extracted from the leaf of B. glabra by hydrodistillation in an all glass Clevenger-type apparatus and characterized by gas chromatography (GC-FID) and gas chromatography-mass spectrometry (GC-MS) on HP-5 column.
30576771	12	47	theme	anti-nociceptive	2035:2050	arg1	properties					2074:2083	the anti-nociceptive and anti-inflammatory properties	2031:2083	the anti-nociceptive and anti-inflammatory properties traditionally ascribed to B. glabra	2031:2119	Overall, the results herein presented sustain and strengthen the anti-nociceptive and anti-inflammatory properties traditionally ascribed to B. glabra.
30576771	1	48	theme	pain	264:267	arg1	ailments					269:276	pain ailments	264:276	pain ailments	264:276	ETHNOPHARMACOLOGICAL RELEVANCE Bougainvillea glabra is widely used in Nigeria for the treatment of inflammatory-related conditions, and as remedy for pain ailments.
30576771	11	49	from	report	1866:1871	arg1	constituents					1889:1900	the chemical constituents	1876:1900	the chemical constituents	1876:1900	CONCLUSIONS This is first report on the chemical constituents and biological activity of essential oil of B. glabra from Nigeria.
30576771	11	49	from	report	1866:1871	arg1	Nigeria					1961:1967	Nigeria	1961:1967	Nigeria	1961:1967	CONCLUSIONS This is first report on the chemical constituents and biological activity of essential oil of B. glabra from Nigeria.
30576771	3	50	theme	gas	754:756	arg1	GC-FID					774:779	GC-FID	774:779	GC-FID	774:779	MATERIALS AND METHODS The essential oil was extracted from the leaf of B. glabra by hydrodistillation in an all glass Clevenger-type apparatus and characterized by gas chromatography (GC-FID) and gas chromatography-mass spectrometry (GC-MS) on HP-5 column.
30576771	3	50	theme	gas	754:756	arg1	chromatography					758:771	gas chromatography	754:771	gas chromatography (GC-FID)	754:780	MATERIALS AND METHODS The essential oil was extracted from the leaf of B. glabra by hydrodistillation in an all glass Clevenger-type apparatus and characterized by gas chromatography (GC-FID) and gas chromatography-mass spectrometry (GC-MS) on HP-5 column.
30576771	3	51	theme	gas	786:788	arg1	GC-MS					824:828	GC-MS	824:828	GC-MS	824:828	MATERIALS AND METHODS The essential oil was extracted from the leaf of B. glabra by hydrodistillation in an all glass Clevenger-type apparatus and characterized by gas chromatography (GC-FID) and gas chromatography-mass spectrometry (GC-MS) on HP-5 column.
30576771	3	51	theme	gas	786:788	arg1	spectrometry					810:821	gas chromatography-mass spectrometry	786:821	gas chromatography-mass spectrometry (GC-MS)	786:829	MATERIALS AND METHODS The essential oil was extracted from the leaf of B. glabra by hydrodistillation in an all glass Clevenger-type apparatus and characterized by gas chromatography (GC-FID) and gas chromatography-mass spectrometry (GC-MS) on HP-5 column.
30576771	11	52	theme	biological	1906:1915	arg1	activity					1917:1924	biological activity	1906:1924	biological activity of essential oil of B. glabra from Nigeria	1906:1967	CONCLUSIONS This is first report on the chemical constituents and biological activity of essential oil of B. glabra from Nigeria.
30576771	4	53	theme	essential	885:893	arg1	property					869:876	The anti-inflammatory property	847:876	The anti-inflammatory property of the essential	847:893	The anti-inflammatory property of the essential was established by measurement of carrageenan induced rat paw edema while the anti-nociceptive activity was determined by hot plate test, according to established procedures.
30576771	6	54	theme	compounds	1181:1189	arg1	total					1169:1173	A total	1167:1173	A total of 11 compounds representing 96.2% of the oil contents	1167:1228	A total of 11 compounds representing 96.2% of the oil contents were identified by GC/MS.
30576771	3	55	theme	essential	616:624	arg1	oil					626:628	The essential oil	612:628	The essential oil	612:628	MATERIALS AND METHODS The essential oil was extracted from the leaf of B. glabra by hydrodistillation in an all glass Clevenger-type apparatus and characterized by gas chromatography (GC-FID) and gas chromatography-mass spectrometry (GC-MS) on HP-5 column.
30576771	2	56	theme	scientific	320:329	arg1	studies					331:337	scientific studies	320:337	scientific studies focused on chemical constituents	320:370	AIM OF THE STUDY Considering the lack of scientific studies focused on chemical constituents and pharmacological activity of B. glabra essential oil, this work was designed to characterize the volatile compounds and evaluate the anti-inflammatory and anti-nociceptive properties ascribed to this plant species.
30576771	1	57	theme	conditions	234:243	arg1	treatment					200:208	the treatment	196:208	the treatment of inflammatory-related conditions	196:243	ETHNOPHARMACOLOGICAL RELEVANCE Bougainvillea glabra is widely used in Nigeria for the treatment of inflammatory-related conditions, and as remedy for pain ailments.
30576771	5	58	dep	RESULTS	1070:1076	arg1	obtained					1100:1107	obtained	1100:1107	was obtained in a yield of 0.08% (v/w) calculated on dry weight basis	1096:1164	RESULTS The essential oil was obtained in a yield of 0.08% (v/w) calculated on dry weight basis.
30576771	10	59	theme	h	1815:1815	arg1	duration					1830:1837	the 4th h experimental duration	1807:1837	the 4th h experimental duration	1807:1837	The activity was non-significant at the 4th h experimental duration.
30576771	11	60	theme	essential	1929:1937	arg1	oil					1939:1941	essential oil	1929:1941	essential oil of B. glabra from Nigeria	1929:1967	CONCLUSIONS This is first report on the chemical constituents and biological activity of essential oil of B. glabra from Nigeria.
30576771	1	61	theme	Bougainvillea	145:157	arg1	remedy					253:258	remedy	253:258	remedy	253:258	ETHNOPHARMACOLOGICAL RELEVANCE Bougainvillea glabra is widely used in Nigeria for the treatment of inflammatory-related conditions, and as remedy for pain ailments.
30576771	1	61	theme	Bougainvillea	145:157	arg1	RELEVANCE					135:143	ETHNOPHARMACOLOGICAL RELEVANCE	114:143	ETHNOPHARMACOLOGICAL RELEVANCE Bougainvillea glabra	114:164	ETHNOPHARMACOLOGICAL RELEVANCE Bougainvillea glabra is widely used in Nigeria for the treatment of inflammatory-related conditions, and as remedy for pain ailments.
30576771	8	62	theme	significant	1439:1449	arg1	property					1391:1398	The anti-nociceptive property	1370:1398	The anti-nociceptive property of the essential oil	1370:1419	The anti-nociceptive property of the essential oil was statistically significant p < 0.001 at all doses of when compared to the control while exhibiting an activity in tandem with the standard drug.
30576771	8	62	theme	significant	1439:1449	arg1	p < 0.001					1451:1459	statistically significant p < 0.001	1425:1459	statistically significant p < 0.001	1425:1459	The anti-nociceptive property of the essential oil was statistically significant p < 0.001 at all doses of when compared to the control while exhibiting an activity in tandem with the standard drug.
30576771	11	63	theme	oil	1939:1941	arg1	report					1866:1871	first report	1860:1871	first report on the chemical constituents	1860:1900	CONCLUSIONS This is first report on the chemical constituents and biological activity of essential oil of B. glabra from Nigeria.
30576771	11	63	theme	oil	1939:1941	arg1	activity					1917:1924	biological activity	1906:1924	biological activity of essential oil of B. glabra from Nigeria	1906:1967	CONCLUSIONS This is first report on the chemical constituents and biological activity of essential oil of B. glabra from Nigeria.
30576771	9	64	dep	significant	1677:1687	arg1	activity					1645:1652	the anti-inflammatory activity	1623:1652	the anti-inflammatory activity	1623:1652	For the 1st and 2nd h, at doses of 100 and 200 mg/kg, the anti-inflammatory activity was statistically very significant (p < 0.001), while at 3rd h the activity declined (p < 0.01) at a dose of 200 mg.
30576771	9	64	dep	significant	1677:1687	arg1	p < 0.001					1690:1698	p < 0.001	1690:1698	p < 0.001	1690:1698	For the 1st and 2nd h, at doses of 100 and 200 mg/kg, the anti-inflammatory activity was statistically very significant (p < 0.001), while at 3rd h the activity declined (p < 0.01) at a dose of 200 mg.
30576771	9	64	dep	significant	1677:1687	arg1	significant					1677:1687	significant	1677:1687	significant	1677:1687	For the 1st and 2nd h, at doses of 100 and 200 mg/kg, the anti-inflammatory activity was statistically very significant (p < 0.001), while at 3rd h the activity declined (p < 0.01) at a dose of 200 mg.
30576771	3	65	dep	MATERIALS	590:598	arg1	oil					626:628	The essential oil	612:628	The essential oil	612:628	MATERIALS AND METHODS The essential oil was extracted from the leaf of B. glabra by hydrodistillation in an all glass Clevenger-type apparatus and characterized by gas chromatography (GC-FID) and gas chromatography-mass spectrometry (GC-MS) on HP-5 column.
30576771	4	66	theme	edema	957:961	arg1	measurement					914:924	measurement	914:924	measurement of carrageenan induced rat paw edema	914:961	The anti-inflammatory property of the essential was established by measurement of carrageenan induced rat paw edema while the anti-nociceptive activity was determined by hot plate test, according to established procedures.
30576771	8	67	theme	oil	1417:1419	arg1	p < 0.001					1451:1459	statistically significant p < 0.001	1425:1459	statistically significant p < 0.001	1425:1459	The anti-nociceptive property of the essential oil was statistically significant p < 0.001 at all doses of when compared to the control while exhibiting an activity in tandem with the standard drug.
30576771	8	67	theme	oil	1417:1419	arg1	property					1391:1398	The anti-nociceptive property	1370:1398	The anti-nociceptive property of the essential oil	1370:1419	The anti-nociceptive property of the essential oil was statistically significant p < 0.001 at all doses of when compared to the control while exhibiting an activity in tandem with the standard drug.
30576771	6	68	theme	oil	1217:1219	arg1	contents					1221:1228	the oil contents	1213:1228	the oil contents	1213:1228	A total of 11 compounds representing 96.2% of the oil contents were identified by GC/MS.
30576771	0	69	theme	oil	85:87	arg1	anti-nociceptive					22:37	anti-nociceptive	22:37	anti-nociceptive	22:37	Chemical composition, anti-nociceptive and anti-inflammatory activities of essential oil of Bougainvillea glabra.
30576771	0	69	theme	oil	85:87	arg1	activities					61:70	anti-inflammatory activities	43:70	anti-inflammatory activities	43:70	Chemical composition, anti-nociceptive and anti-inflammatory activities of essential oil of Bougainvillea glabra.
30576771	0	69	theme	oil	85:87	arg1	composition					9:19	Chemical composition	0:19	Chemical composition	0:19	Chemical composition, anti-nociceptive and anti-inflammatory activities of essential oil of Bougainvillea glabra.
30576771	11	70	dep	CONCLUSIONS	1840:1850	arg1	This					1852:1855	This	1852:1855	This	1852:1855	CONCLUSIONS This is first report on the chemical constituents and biological activity of essential oil of B. glabra from Nigeria.
30576771	2	71	theme	anti-nociceptive	530:545	arg1	properties					547:556	the anti-inflammatory and anti-nociceptive properties	504:556	the anti-inflammatory and anti-nociceptive properties ascribed to this plant species	504:587	AIM OF THE STUDY Considering the lack of scientific studies focused on chemical constituents and pharmacological activity of B. glabra essential oil, this work was designed to characterize the volatile compounds and evaluate the anti-inflammatory and anti-nociceptive properties ascribed to this plant species.
30576771	11	72	from	Nigeria	1961:1967	arg1	oil					1939:1941	essential oil	1929:1941	essential oil of B. glabra from Nigeria	1929:1967	CONCLUSIONS This is first report on the chemical constituents and biological activity of essential oil of B. glabra from Nigeria.
30576771	11	72	from	Nigeria	1961:1967	arg1	report					1866:1871	first report	1860:1871	first report on the chemical constituents	1860:1900	CONCLUSIONS This is first report on the chemical constituents and biological activity of essential oil of B. glabra from Nigeria.
30576771	11	72	from	Nigeria	1961:1967	arg1	activity					1917:1924	biological activity	1906:1924	biological activity of essential oil of B. glabra from Nigeria	1906:1967	CONCLUSIONS This is first report on the chemical constituents and biological activity of essential oil of B. glabra from Nigeria.
30576771	0	73	theme	Bougainvillea	92:104	arg1	glabra					106:111	Bougainvillea glabra	92:111	Bougainvillea glabra	92:111	Chemical composition, anti-nociceptive and anti-inflammatory activities of essential oil of Bougainvillea glabra.
30576771	5	74	theme	weight	1153:1158	arg1	basis					1160:1164	dry weight basis	1149:1164	dry weight basis	1149:1164	RESULTS The essential oil was obtained in a yield of 0.08% (v/w) calculated on dry weight basis.
30576771	2	75	theme	anti-inflammatory	508:524	arg1	properties					547:556	the anti-inflammatory and anti-nociceptive properties	504:556	the anti-inflammatory and anti-nociceptive properties ascribed to this plant species	504:587	AIM OF THE STUDY Considering the lack of scientific studies focused on chemical constituents and pharmacological activity of B. glabra essential oil, this work was designed to characterize the volatile compounds and evaluate the anti-inflammatory and anti-nociceptive properties ascribed to this plant species.
30576771	9	76	from	doses	1595:1599	arg1	activity					1645:1652	the anti-inflammatory activity	1623:1652	the anti-inflammatory activity	1623:1652	For the 1st and 2nd h, at doses of 100 and 200 mg/kg, the anti-inflammatory activity was statistically very significant (p < 0.001), while at 3rd h the activity declined (p < 0.01) at a dose of 200 mg.
30576771	9	76	from	doses	1595:1599	arg1	p < 0.001					1690:1698	p < 0.001	1690:1698	p < 0.001	1690:1698	For the 1st and 2nd h, at doses of 100 and 200 mg/kg, the anti-inflammatory activity was statistically very significant (p < 0.001), while at 3rd h the activity declined (p < 0.01) at a dose of 200 mg.
30576771	9	76	from	doses	1595:1599	arg1	significant					1677:1687	significant	1677:1687	significant	1677:1687	For the 1st and 2nd h, at doses of 100 and 200 mg/kg, the anti-inflammatory activity was statistically very significant (p < 0.001), while at 3rd h the activity declined (p < 0.01) at a dose of 200 mg.
30576771	4	77	theme	hot	1017:1019	arg1	test					1027:1030	hot plate test	1017:1030	hot plate test	1017:1030	The anti-inflammatory property of the essential was established by measurement of carrageenan induced rat paw edema while the anti-nociceptive activity was determined by hot plate test, according to established procedures.
30576771	11	78	theme	glabra	1949:1954	arg1	oil					1939:1941	essential oil	1929:1941	essential oil of B. glabra from Nigeria	1929:1967	CONCLUSIONS This is first report on the chemical constituents and biological activity of essential oil of B. glabra from Nigeria.
30576771	2	79	theme	glabra	407:412	arg1	activity					392:399	pharmacological activity	376:399	pharmacological activity of B. glabra	376:412	AIM OF THE STUDY Considering the lack of scientific studies focused on chemical constituents and pharmacological activity of B. glabra essential oil, this work was designed to characterize the volatile compounds and evaluate the anti-inflammatory and anti-nociceptive properties ascribed to this plant species.
30576771	2	79	theme	glabra	407:412	arg1	lack					312:315	the lack	308:315	the lack of scientific studies focused on chemical constituents	308:370	AIM OF THE STUDY Considering the lack of scientific studies focused on chemical constituents and pharmacological activity of B. glabra essential oil, this work was designed to characterize the volatile compounds and evaluate the anti-inflammatory and anti-nociceptive properties ascribed to this plant species.
30576771	1	80	theme	ETHNOPHARMACOLOGICAL	114:133	arg1	remedy					253:258	remedy	253:258	remedy	253:258	ETHNOPHARMACOLOGICAL RELEVANCE Bougainvillea glabra is widely used in Nigeria for the treatment of inflammatory-related conditions, and as remedy for pain ailments.
30576771	1	80	theme	ETHNOPHARMACOLOGICAL	114:133	arg1	RELEVANCE					135:143	ETHNOPHARMACOLOGICAL RELEVANCE	114:143	ETHNOPHARMACOLOGICAL RELEVANCE Bougainvillea glabra	114:164	ETHNOPHARMACOLOGICAL RELEVANCE Bougainvillea glabra is widely used in Nigeria for the treatment of inflammatory-related conditions, and as remedy for pain ailments.
30576771	7	81	theme	E	1305:1305	arg1	constituents					1265:1276	The main constituents	1256:1276	The main constituents of the essential oil	1256:1297	The main constituents of the essential oil were (E)-nerolidol (31.4%), (E)-β-ionone (10.3%) and linalool (10.1%).
30576771	7	81	theme	E	1305:1305	arg1	-nerolidol					1307:1316	(E)-nerolidol	1304:1316	(E)-nerolidol (31.4%)	1304:1324	The main constituents of the essential oil were (E)-nerolidol (31.4%), (E)-β-ionone (10.3%) and linalool (10.1%).
30576771	7	81	theme	E	1305:1305	arg1	%					1323:1323	31.4%	1319:1323	31.4%	1319:1323	The main constituents of the essential oil were (E)-nerolidol (31.4%), (E)-β-ionone (10.3%) and linalool (10.1%).
30576771	0	82	theme	anti-inflammatory	43:59	arg1	activities					61:70	anti-inflammatory activities	43:70	anti-inflammatory activities	43:70	Chemical composition, anti-nociceptive and anti-inflammatory activities of essential oil of Bougainvillea glabra.
30576771	4	83	theme	established	1046:1056	arg1	procedures					1058:1067	established procedures	1046:1067	established procedures	1046:1067	The anti-inflammatory property of the essential was established by measurement of carrageenan induced rat paw edema while the anti-nociceptive activity was determined by hot plate test, according to established procedures.
30576771	4	84	theme	anti-inflammatory	851:867	arg1	property					869:876	The anti-inflammatory property	847:876	The anti-inflammatory property of the essential	847:893	The anti-inflammatory property of the essential was established by measurement of carrageenan induced rat paw edema while the anti-nociceptive activity was determined by hot plate test, according to established procedures.
30576771	7	85	theme	essential	1285:1293	arg1	oil					1295:1297	the essential oil	1281:1297	the essential oil	1281:1297	The main constituents of the essential oil were (E)-nerolidol (31.4%), (E)-β-ionone (10.3%) and linalool (10.1%).
30576771	3	86	theme	Clevenger-type	708:721	arg1	apparatus					723:731	an all glass Clevenger-type apparatus	695:731	an all glass Clevenger-type apparatus	695:731	MATERIALS AND METHODS The essential oil was extracted from the leaf of B. glabra by hydrodistillation in an all glass Clevenger-type apparatus and characterized by gas chromatography (GC-FID) and gas chromatography-mass spectrometry (GC-MS) on HP-5 column.
30576771	3	87	theme	all	698:700	arg1	apparatus					723:731	an all glass Clevenger-type apparatus	695:731	an all glass Clevenger-type apparatus	695:731	MATERIALS AND METHODS The essential oil was extracted from the leaf of B. glabra by hydrodistillation in an all glass Clevenger-type apparatus and characterized by gas chromatography (GC-FID) and gas chromatography-mass spectrometry (GC-MS) on HP-5 column.
30576771	11	88	theme	first	1860:1864	arg1	report					1866:1871	first report	1860:1871	first report on the chemical constituents	1860:1900	CONCLUSIONS This is first report on the chemical constituents and biological activity of essential oil of B. glabra from Nigeria.
30576771	7	89	theme	main	1260:1263	arg1	constituents					1265:1276	The main constituents	1256:1276	The main constituents of the essential oil	1256:1297	The main constituents of the essential oil were (E)-nerolidol (31.4%), (E)-β-ionone (10.3%) and linalool (10.1%).
30576771	7	89	theme	main	1260:1263	arg1	-nerolidol					1307:1316	(E)-nerolidol	1304:1316	(E)-nerolidol (31.4%)	1304:1324	The main constituents of the essential oil were (E)-nerolidol (31.4%), (E)-β-ionone (10.3%) and linalool (10.1%).
30576771	11	90	from	activity	1917:1924	arg1	constituents					1889:1900	the chemical constituents	1876:1900	the chemical constituents	1876:1900	CONCLUSIONS This is first report on the chemical constituents and biological activity of essential oil of B. glabra from Nigeria.
30576771	11	90	from	activity	1917:1924	arg1	Nigeria					1961:1967	Nigeria	1961:1967	Nigeria	1961:1967	CONCLUSIONS This is first report on the chemical constituents and biological activity of essential oil of B. glabra from Nigeria.
31187943	4	0	from	aspect	524:529	arg1	development					540:550	plant development	534:550	plant development	534:550	The composition and assembly of the cell wall is an important aspect in plant development and plant biomass utilization.
31187943	4	0	from	aspect	524:529	arg1	utilization					570:580	plant biomass utilization	556:580	plant biomass utilization	556:580	The composition and assembly of the cell wall is an important aspect in plant development and plant biomass utilization.
31187943	5	1	from	sugars	756:761	arg1	wall					775:778	the cell wall	766:778	the cell wall from a single run	766:796	The application of anion-exchange chromatography to determine the monosaccharide composition of the insoluble matrix polysaccharides enables a complete profile of all major sugars in the cell wall from a single run.
31187943	5	1	from	sugars	756:761	arg1	run					794:796	a single run	785:796	a single run	785:796	The application of anion-exchange chromatography to determine the monosaccharide composition of the insoluble matrix polysaccharides enables a complete profile of all major sugars in the cell wall from a single run.
31187943	8	2	theme	Wiley	1125:1129	arg1	Sons					1133:1136	John Wiley & Sons	1120:1136	John Wiley & Sons	1120:1136	© 2019 by John Wiley & Sons, Inc.
31187943	5	3	theme	matrix	693:698	arg1	polysaccharides					700:714	the insoluble matrix polysaccharides	679:714	the insoluble matrix polysaccharides	679:714	The application of anion-exchange chromatography to determine the monosaccharide composition of the insoluble matrix polysaccharides enables a complete profile of all major sugars in the cell wall from a single run.
31187943	5	4	theme	chromatography	617:630	arg1	application					587:597	The application	583:597	The application of anion-exchange chromatography to determine the monosaccharide composition of the insoluble matrix polysaccharides	583:714	The application of anion-exchange chromatography to determine the monosaccharide composition of the insoluble matrix polysaccharides enables a complete profile of all major sugars in the cell wall from a single run.
31187943	5	5	theme	insoluble	683:691	arg1	polysaccharides					700:714	the insoluble matrix polysaccharides	679:714	the insoluble matrix polysaccharides	679:714	The application of anion-exchange chromatography to determine the monosaccharide composition of the insoluble matrix polysaccharides enables a complete profile of all major sugars in the cell wall from a single run.
31187943	4	6	theme	important	514:522	arg1	composition					466:476	composition	466:476	composition	466:476	The composition and assembly of the cell wall is an important aspect in plant development and plant biomass utilization.
31187943	4	6	theme	important	514:522	arg1	assembly					482:489	assembly	482:489	assembly	482:489	The composition and assembly of the cell wall is an important aspect in plant development and plant biomass utilization.
31187943	4	6	theme	important	514:522	arg1	aspect					524:529	an important aspect	511:529	an important aspect in plant development and plant biomass utilization	511:580	The composition and assembly of the cell wall is an important aspect in plant development and plant biomass utilization.
31187943	5	7	theme	polysaccharides	700:714	arg1	composition					664:674	the monosaccharide composition	645:674	the monosaccharide composition of the insoluble matrix polysaccharides	645:714	The application of anion-exchange chromatography to determine the monosaccharide composition of the insoluble matrix polysaccharides enables a complete profile of all major sugars in the cell wall from a single run.
31187943	6	8	theme	tandem	847:852	arg1	spectrometry					859:870	tandem mass spectrometry	847:870	tandem mass spectrometry	847:870	While porous carbon graphite chromatography and tandem mass spectrometry delivers a sensitive and robust nucleotide sugar profile from plant extracts.
31187943	7	9	theme	detailed	967:974	arg1	methodology					976:986	detailed methodology	967:986	detailed methodology to quantify nucleotide sugars within the cell and profile the non-cellulosic monosaccharide composition of the cell wall	967:1107	Here we describe detailed methodology to quantify nucleotide sugars within the cell and profile the non-cellulosic monosaccharide composition of the cell wall.
31187943	4	10	dep	composition	466:476	arg1	The					462:464	The	462:464	The	462:464	The composition and assembly of the cell wall is an important aspect in plant development and plant biomass utilization.
31187943	5	11	theme	cell	770:773	arg1	wall					775:778	the cell wall	766:778	the cell wall from a single run	766:796	The application of anion-exchange chromatography to determine the monosaccharide composition of the insoluble matrix polysaccharides enables a complete profile of all major sugars in the cell wall from a single run.
31187943	0	12	from	Plants	63:68	arg1	Nucleotide-Sugars					40:56	Nucleotide-Sugars	40:56	Nucleotide-Sugars from Plants	40:68	Profiling Cell Wall Monosaccharides and Nucleotide-Sugars from Plants.
31187943	0	12	from	Plants	63:68	arg1	Monosaccharides					20:34	Cell Wall Monosaccharides	10:34	Cell Wall Monosaccharides	10:34	Profiling Cell Wall Monosaccharides and Nucleotide-Sugars from Plants.
31187943	7	13	theme	nucleotide	1000:1009	arg1	sugars					1011:1016	nucleotide sugars	1000:1016	nucleotide sugars within the cell and profile the non-cellulosic monosaccharide composition of the cell wall	1000:1107	Here we describe detailed methodology to quantify nucleotide sugars within the cell and profile the non-cellulosic monosaccharide composition of the cell wall.
31187943	6	14	theme	plant	934:938	arg1	extracts					940:947	plant extracts	934:947	plant extracts	934:947	While porous carbon graphite chromatography and tandem mass spectrometry delivers a sensitive and robust nucleotide sugar profile from plant extracts.
31187943	5	15	theme	monosaccharide	649:662	arg1	composition					664:674	the monosaccharide composition	645:674	the monosaccharide composition of the insoluble matrix polysaccharides	645:714	The application of anion-exchange chromatography to determine the monosaccharide composition of the insoluble matrix polysaccharides enables a complete profile of all major sugars in the cell wall from a single run.
31187943	1	16	theme	cell	75:78	arg1	wall					80:83	The cell wall	71:83	The cell wall	71:83	The cell wall is an intricate mesh largely composed of polysaccharides that vary in structure and abundance.
31187943	1	16	theme	cell	75:78	arg1	mesh					101:104	an intricate mesh	88:104	an intricate mesh largely composed of polysaccharides that vary in structure and abundance	88:177	The cell wall is an intricate mesh largely composed of polysaccharides that vary in structure and abundance.
31187943	4	17	theme	wall	503:506	arg1	composition					466:476	composition	466:476	composition	466:476	The composition and assembly of the cell wall is an important aspect in plant development and plant biomass utilization.
31187943	4	17	theme	wall	503:506	arg1	aspect					524:529	an important aspect	511:529	an important aspect in plant development and plant biomass utilization	511:580	The composition and assembly of the cell wall is an important aspect in plant development and plant biomass utilization.
31187943	4	17	theme	wall	503:506	arg1	assembly					482:489	assembly	482:489	assembly	482:489	The composition and assembly of the cell wall is an important aspect in plant development and plant biomass utilization.
31187943	6	18	theme	graphite	819:826	arg1	chromatography					828:841	porous carbon graphite chromatography	805:841	porous carbon graphite chromatography	805:841	While porous carbon graphite chromatography and tandem mass spectrometry delivers a sensitive and robust nucleotide sugar profile from plant extracts.
31187943	0	19	theme	Cell	10:13	arg1	Monosaccharides					20:34	Cell Wall Monosaccharides	10:34	Cell Wall Monosaccharides	10:34	Profiling Cell Wall Monosaccharides and Nucleotide-Sugars from Plants.
31187943	4	20	theme	cell	498:501	arg1	wall					503:506	the cell wall	494:506	the cell wall	494:506	The composition and assembly of the cell wall is an important aspect in plant development and plant biomass utilization.
31187943	8	21	theme	John	1120:1123	arg1	Sons					1133:1136	John Wiley & Sons	1120:1136	John Wiley & Sons	1120:1136	© 2019 by John Wiley & Sons, Inc.
31187943	6	22	theme	carbon	812:817	arg1	chromatography					828:841	porous carbon graphite chromatography	805:841	porous carbon graphite chromatography	805:841	While porous carbon graphite chromatography and tandem mass spectrometry delivers a sensitive and robust nucleotide sugar profile from plant extracts.
31187943	4	23	theme	biomass	562:568	arg1	utilization					570:580	plant biomass utilization	556:580	plant biomass utilization	556:580	The composition and assembly of the cell wall is an important aspect in plant development and plant biomass utilization.
31187943	6	24	theme	porous	805:810	arg1	chromatography					828:841	porous carbon graphite chromatography	805:841	porous carbon graphite chromatography	805:841	While porous carbon graphite chromatography and tandem mass spectrometry delivers a sensitive and robust nucleotide sugar profile from plant extracts.
31187943	6	25	theme	sensitive	883:891	arg1	profile					921:927	a sensitive and robust nucleotide sugar profile	881:927	a sensitive and robust nucleotide sugar profile	881:927	While porous carbon graphite chromatography and tandem mass spectrometry delivers a sensitive and robust nucleotide sugar profile from plant extracts.
31187943	7	26	dep	cell	1029:1032	arg1	composition					1080:1090	the non-cellulosic monosaccharide composition	1046:1090	the non-cellulosic monosaccharide composition	1046:1090	Here we describe detailed methodology to quantify nucleotide sugars within the cell and profile the non-cellulosic monosaccharide composition of the cell wall.
31187943	7	26	dep	cell	1029:1032	arg1	the					1025:1027	the	1025:1027	the	1025:1027	Here we describe detailed methodology to quantify nucleotide sugars within the cell and profile the non-cellulosic monosaccharide composition of the cell wall.
31187943	3	27	theme	nucleotide	443:452	arg1	sugars					454:459	nucleotide sugars	443:459	nucleotide sugars	443:459	Matrix polysaccharides are biosynthesized from activated precursors or nucleotide sugars.
31187943	4	28	theme	plant	556:560	arg1	utilization					570:580	plant biomass utilization	556:580	plant biomass utilization	556:580	The composition and assembly of the cell wall is an important aspect in plant development and plant biomass utilization.
31187943	8	29	theme	&	1131:1131	arg1	Sons					1133:1136	John Wiley & Sons	1120:1136	John Wiley & Sons	1120:1136	© 2019 by John Wiley & Sons, Inc.
31187943	5	30	theme	anion-exchange	602:615	arg1	chromatography					617:630	anion-exchange chromatography	602:630	anion-exchange chromatography	602:630	The application of anion-exchange chromatography to determine the monosaccharide composition of the insoluble matrix polysaccharides enables a complete profile of all major sugars in the cell wall from a single run.
31187943	2	31	theme	Golgi	300:304	arg1	apparatus					306:314	the Golgi apparatus	296:314	the Golgi apparatus	296:314	Apart from cellulose biosynthesis, the assembly of matrix polysaccharides such as pectin and hemicellulose occur in the Golgi apparatus before being transported via vesicles to the cell wall.
31187943	6	32	theme	mass	854:857	arg1	spectrometry					859:870	tandem mass spectrometry	847:870	tandem mass spectrometry	847:870	While porous carbon graphite chromatography and tandem mass spectrometry delivers a sensitive and robust nucleotide sugar profile from plant extracts.
31187943	3	33	theme	Matrix	372:377	arg1	polysaccharides					379:393	Matrix polysaccharides	372:393	Matrix polysaccharides	372:393	Matrix polysaccharides are biosynthesized from activated precursors or nucleotide sugars.
31187943	5	34	theme	major	750:754	arg1	sugars					756:761	all major sugars	746:761	all major sugars in the cell wall from a single run	746:796	The application of anion-exchange chromatography to determine the monosaccharide composition of the insoluble matrix polysaccharides enables a complete profile of all major sugars in the cell wall from a single run.
31187943	5	35	from	wall	775:778	arg1	profile					735:741	a complete profile	724:741	a complete profile of all major sugars in the cell wall from a single run	724:796	The application of anion-exchange chromatography to determine the monosaccharide composition of the insoluble matrix polysaccharides enables a complete profile of all major sugars in the cell wall from a single run.
31187943	3	36	theme	activated	419:427	arg1	precursors					429:438	activated precursors	419:438	activated precursors	419:438	Matrix polysaccharides are biosynthesized from activated precursors or nucleotide sugars.
31187943	5	37	theme	sugars	756:761	arg1	profile					735:741	a complete profile	724:741	a complete profile of all major sugars in the cell wall from a single run	724:796	The application of anion-exchange chromatography to determine the monosaccharide composition of the insoluble matrix polysaccharides enables a complete profile of all major sugars in the cell wall from a single run.
31187943	6	38	theme	nucleotide	904:913	arg1	profile					921:927	a sensitive and robust nucleotide sugar profile	881:927	a sensitive and robust nucleotide sugar profile	881:927	While porous carbon graphite chromatography and tandem mass spectrometry delivers a sensitive and robust nucleotide sugar profile from plant extracts.
31187943	2	39	theme	matrix	231:236	arg1	hemicellulose					273:285	hemicellulose	273:285	hemicellulose	273:285	Apart from cellulose biosynthesis, the assembly of matrix polysaccharides such as pectin and hemicellulose occur in the Golgi apparatus before being transported via vesicles to the cell wall.
31187943	2	39	theme	matrix	231:236	arg1	polysaccharides					238:252	matrix polysaccharides	231:252	matrix polysaccharides such as pectin and hemicellulose	231:285	Apart from cellulose biosynthesis, the assembly of matrix polysaccharides such as pectin and hemicellulose occur in the Golgi apparatus before being transported via vesicles to the cell wall.
31187943	2	39	theme	matrix	231:236	arg1	pectin					262:267	pectin	262:267	pectin	262:267	Apart from cellulose biosynthesis, the assembly of matrix polysaccharides such as pectin and hemicellulose occur in the Golgi apparatus before being transported via vesicles to the cell wall.
31187943	0	40	theme	Wall	15:18	arg1	Monosaccharides					20:34	Cell Wall Monosaccharides	10:34	Cell Wall Monosaccharides	10:34	Profiling Cell Wall Monosaccharides and Nucleotide-Sugars from Plants.
31187943	4	41	theme	plant	534:538	arg1	development					540:550	plant development	534:550	plant development	534:550	The composition and assembly of the cell wall is an important aspect in plant development and plant biomass utilization.
31187943	6	42	theme	robust	897:902	arg1	profile					921:927	a sensitive and robust nucleotide sugar profile	881:927	a sensitive and robust nucleotide sugar profile	881:927	While porous carbon graphite chromatography and tandem mass spectrometry delivers a sensitive and robust nucleotide sugar profile from plant extracts.
31187943	7	43	theme	wall	1104:1107	arg1	profile					1038:1044	profile	1038:1044	profile	1038:1044	Here we describe detailed methodology to quantify nucleotide sugars within the cell and profile the non-cellulosic monosaccharide composition of the cell wall.
31187943	7	43	theme	wall	1104:1107	arg1	cell					1029:1032	cell	1029:1032	cell	1029:1032	Here we describe detailed methodology to quantify nucleotide sugars within the cell and profile the non-cellulosic monosaccharide composition of the cell wall.
31187943	6	44	theme	sugar	915:919	arg1	profile					921:927	a sensitive and robust nucleotide sugar profile	881:927	a sensitive and robust nucleotide sugar profile	881:927	While porous carbon graphite chromatography and tandem mass spectrometry delivers a sensitive and robust nucleotide sugar profile from plant extracts.
31187943	5	45	theme	complete	726:733	arg1	profile					735:741	a complete profile	724:741	a complete profile of all major sugars in the cell wall from a single run	724:796	The application of anion-exchange chromatography to determine the monosaccharide composition of the insoluble matrix polysaccharides enables a complete profile of all major sugars in the cell wall from a single run.
31187943	5	46	from	run	794:796	arg1	wall					775:778	the cell wall	766:778	the cell wall from a single run	766:796	The application of anion-exchange chromatography to determine the monosaccharide composition of the insoluble matrix polysaccharides enables a complete profile of all major sugars in the cell wall from a single run.
31187943	5	46	from	run	794:796	arg1	sugars					756:761	all major sugars	746:761	all major sugars in the cell wall from a single run	746:796	The application of anion-exchange chromatography to determine the monosaccharide composition of the insoluble matrix polysaccharides enables a complete profile of all major sugars in the cell wall from a single run.
31187943	7	47	theme	non-cellulosic	1050:1063	arg1	composition					1080:1090	the non-cellulosic monosaccharide composition	1046:1090	the non-cellulosic monosaccharide composition	1046:1090	Here we describe detailed methodology to quantify nucleotide sugars within the cell and profile the non-cellulosic monosaccharide composition of the cell wall.
31187943	7	48	theme	monosaccharide	1065:1078	arg1	composition					1080:1090	the non-cellulosic monosaccharide composition	1046:1090	the non-cellulosic monosaccharide composition	1046:1090	Here we describe detailed methodology to quantify nucleotide sugars within the cell and profile the non-cellulosic monosaccharide composition of the cell wall.
31187943	1	49	theme	intricate	91:99	arg1	wall					80:83	The cell wall	71:83	The cell wall	71:83	The cell wall is an intricate mesh largely composed of polysaccharides that vary in structure and abundance.
31187943	1	49	theme	intricate	91:99	arg1	mesh					101:104	an intricate mesh	88:104	an intricate mesh largely composed of polysaccharides that vary in structure and abundance	88:177	The cell wall is an intricate mesh largely composed of polysaccharides that vary in structure and abundance.
31187943	5	50	from	profile	735:741	arg1	wall					775:778	the cell wall	766:778	the cell wall from a single run	766:796	The application of anion-exchange chromatography to determine the monosaccharide composition of the insoluble matrix polysaccharides enables a complete profile of all major sugars in the cell wall from a single run.
31187943	7	51	theme	cell	1099:1102	arg1	wall					1104:1107	the cell wall	1095:1107	the cell wall	1095:1107	Here we describe detailed methodology to quantify nucleotide sugars within the cell and profile the non-cellulosic monosaccharide composition of the cell wall.
31187943	2	52	theme	cellulose	191:199	arg1	biosynthesis					201:212	cellulose biosynthesis	191:212	cellulose biosynthesis	191:212	Apart from cellulose biosynthesis, the assembly of matrix polysaccharides such as pectin and hemicellulose occur in the Golgi apparatus before being transported via vesicles to the cell wall.
31187943	5	53	theme	single	787:792	arg1	run					794:796	a single run	785:796	a single run	785:796	The application of anion-exchange chromatography to determine the monosaccharide composition of the insoluble matrix polysaccharides enables a complete profile of all major sugars in the cell wall from a single run.
31187943	2	54	theme	polysaccharides	238:252	arg1	assembly					219:226	the assembly	215:226	the assembly of matrix polysaccharides such as pectin and hemicellulose	215:285	Apart from cellulose biosynthesis, the assembly of matrix polysaccharides such as pectin and hemicellulose occur in the Golgi apparatus before being transported via vesicles to the cell wall.
31187943	2	55	theme	cell	361:364	arg1	wall					366:369	the cell wall	357:369	the cell wall	357:369	Apart from cellulose biosynthesis, the assembly of matrix polysaccharides such as pectin and hemicellulose occur in the Golgi apparatus before being transported via vesicles to the cell wall.
31461974	3	0	theme	TCM	548:550	arg1	Tang					523:526	Tang	523:526	Tang	523:526	Huangqi Jianzhong Tang (HQJZT) is a famous TCM herbal formula composed of Radix Astragali, Ramulus Cinnamomi, Radix et Rhizoma Glycyrrhizae Praeparata cum Melle, Radix Paeoniae Alba, Rhizoma Zingiberis Recens, Fructus Jujubae and Saccharum Granorum (maltose), which has been used for the treatment of various chronic inflammatory gastrointestinal diseases.
31461974	3	0	theme	TCM	548:550	arg1	formula					559:565	a famous TCM herbal formula	539:565	a famous TCM herbal formula composed of Radix Astragali, Ramulus Cinnamomi, Radix et Rhizoma Glycyrrhizae Praeparata cum Melle, Radix Paeoniae Alba, Rhizoma Zingiberis Recens, Fructus Jujubae and Saccharum Granorum (maltose), which has been used for the treatment of various chronic inflammatory gastrointestinal diseases	539:859	Huangqi Jianzhong Tang (HQJZT) is a famous TCM herbal formula composed of Radix Astragali, Ramulus Cinnamomi, Radix et Rhizoma Glycyrrhizae Praeparata cum Melle, Radix Paeoniae Alba, Rhizoma Zingiberis Recens, Fructus Jujubae and Saccharum Granorum (maltose), which has been used for the treatment of various chronic inflammatory gastrointestinal diseases.
31461974	9	1	theme	Phytochemical	1465:1477	arg1	data					1511:1514	Phytochemical and pharmacological bioactivity data	1465:1514	Phytochemical and pharmacological bioactivity data	1465:1514	Phytochemical and pharmacological bioactivity data were correlated by orthogonal projection to latent structures discriminant analysis (OPLS-DA) in order to identify those HQJZT constituents which may be relevant for the observed pharmacological activities.
31461974	4	2	theme	active	913:918	arg1	constituents					920:931	its active constituents	909:931	its active constituents	909:931	However, there is insufficient knowledge about its active constituents and the mechanisms responsible for its effects.
31461974	5	3	dep	in	1087:1088	arg1	vitro					1090:1094	vitro	1090:1094	vitro	1090:1094	The present study aimed at identifying constituents contributing to the bioactivity of HQJZT by combining in vitro cytokine production assays and LC-MS metabolomics techniques.
31461974	14	4	theme	mixture	2485:2491	arg1	activity					2467:2474	the activity	2463:2474	the activity of a TCM mixture	2463:2491	Moreover, the benefits and limitations of MVDA for prediction pharmacologically active compounds contributing to the activity of a TCM mixture could be detected.
31461974	1	5	theme	challenges	318:327	arg1	one					299:301	one	299:301	one	299:301	Within non-communicable diseases, chronic inflammatory conditions represent one of the biggest challenges for modern medicine.
31461974	1	5	theme	challenges	318:327	arg1	challenges					318:327	the biggest challenges	306:327	the biggest challenges for modern medicine	306:347	Within non-communicable diseases, chronic inflammatory conditions represent one of the biggest challenges for modern medicine.
31461974	12	6	theme	same	2106:2109	arg1	assays					2127:2132	the same pharmacological assays	2102:2132	the same pharmacological assays as for the extracts	2102:2152	The predicted results were verified by testing these compounds in the same pharmacological assays as for the extracts.
31461974	5	7	theme	metabolomics	1133:1144	arg1	techniques					1146:1155	LC-MS metabolomics techniques	1127:1155	LC-MS metabolomics techniques	1127:1155	The present study aimed at identifying constituents contributing to the bioactivity of HQJZT by combining in vitro cytokine production assays and LC-MS metabolomics techniques.
31461974	4	8	theme	insufficient	880:891	arg1	knowledge					893:901	insufficient knowledge	880:901	insufficient knowledge about its active constituents and the mechanisms responsible for its effects	880:978	However, there is insufficient knowledge about its active constituents and the mechanisms responsible for its effects.
31461974	6	9	theme	single	1203:1208	arg1	components					1217:1226	its single herbal components	1199:1226	its single herbal components	1199:1226	From the HQJZT decoction as well as from its single herbal components, extracts of different polarities were prepared.
31461974	11	10	theme	them	1881:1884	arg1	them					1881:1884	them	1881:1884	them	1881:1884	Seven of them, namely calycosin, formononetin, astragaloside I, liquiritigenin, 18β-glycyrrhetinic acid, paeoniflorin and albiflorin were unambiguously identified.
31461974	11	10	theme	them	1881:1884	arg1	I					1933:1933	astragaloside I	1919:1933	astragaloside I	1919:1933	Seven of them, namely calycosin, formononetin, astragaloside I, liquiritigenin, 18β-glycyrrhetinic acid, paeoniflorin and albiflorin were unambiguously identified.
31461974	11	10	theme	them	1881:1884	arg1	paeoniflorin					1977:1988	paeoniflorin	1977:1988	paeoniflorin	1977:1988	Seven of them, namely calycosin, formononetin, astragaloside I, liquiritigenin, 18β-glycyrrhetinic acid, paeoniflorin and albiflorin were unambiguously identified.
31461974	11	10	theme	them	1881:1884	arg1	calycosin					1894:1902	calycosin	1894:1902	calycosin	1894:1902	Seven of them, namely calycosin, formononetin, astragaloside I, liquiritigenin, 18β-glycyrrhetinic acid, paeoniflorin and albiflorin were unambiguously identified.
31461974	11	10	theme	them	1881:1884	arg1	albiflorin					1994:2003	albiflorin	1994:2003	albiflorin	1994:2003	Seven of them, namely calycosin, formononetin, astragaloside I, liquiritigenin, 18β-glycyrrhetinic acid, paeoniflorin and albiflorin were unambiguously identified.
31461974	11	10	theme	them	1881:1884	arg1	liquiritigenin					1936:1949	liquiritigenin	1936:1949	liquiritigenin	1936:1949	Seven of them, namely calycosin, formononetin, astragaloside I, liquiritigenin, 18β-glycyrrhetinic acid, paeoniflorin and albiflorin were unambiguously identified.
31461974	11	10	theme	them	1881:1884	arg1	formononetin					1905:1916	formononetin	1905:1916	formononetin	1905:1916	Seven of them, namely calycosin, formononetin, astragaloside I, liquiritigenin, 18β-glycyrrhetinic acid, paeoniflorin and albiflorin were unambiguously identified.
31461974	11	10	theme	them	1881:1884	arg1	Seven					1872:1876	Seven	1872:1876	Seven	1872:1876	Seven of them, namely calycosin, formononetin, astragaloside I, liquiritigenin, 18β-glycyrrhetinic acid, paeoniflorin and albiflorin were unambiguously identified.
31461974	11	10	theme	them	1881:1884	arg1	acid					1971:1974	18β-glycyrrhetinic acid	1952:1974	18β-glycyrrhetinic acid	1952:1974	Seven of them, namely calycosin, formononetin, astragaloside I, liquiritigenin, 18β-glycyrrhetinic acid, paeoniflorin and albiflorin were unambiguously identified.
31461974	14	11	dep	benefits	2364:2371	arg1	the					2360:2362	the	2360:2362	the	2360:2362	Moreover, the benefits and limitations of MVDA for prediction pharmacologically active compounds contributing to the activity of a TCM mixture could be detected.
31461974	2	12	theme	Traditional	350:360	arg1	TCM					380:382	TCM	380:382	TCM	380:382	Traditional Chinese Medicine (TCM) has been practiced over centuries and has accumulated tremendous empirical knowledge on the treatment of such diseases.
31461974	2	12	theme	Traditional	350:360	arg1	Medicine					370:377	Traditional Chinese Medicine	350:377	Traditional Chinese Medicine (TCM)	350:383	Traditional Chinese Medicine (TCM) has been practiced over centuries and has accumulated tremendous empirical knowledge on the treatment of such diseases.
31461974	9	13	theme	orthogonal	1535:1544	arg1	OPLS-DA					1601:1607	OPLS-DA	1601:1607	OPLS-DA	1601:1607	Phytochemical and pharmacological bioactivity data were correlated by orthogonal projection to latent structures discriminant analysis (OPLS-DA) in order to identify those HQJZT constituents which may be relevant for the observed pharmacological activities.
31461974	9	13	theme	orthogonal	1535:1544	arg1	analysis					1591:1598	orthogonal projection to latent structures discriminant analysis	1535:1598	orthogonal projection to latent structures discriminant analysis (OPLS-DA)	1535:1608	Phytochemical and pharmacological bioactivity data were correlated by orthogonal projection to latent structures discriminant analysis (OPLS-DA) in order to identify those HQJZT constituents which may be relevant for the observed pharmacological activities.
31461974	12	14	theme	predicted	2040:2048	arg1	results					2050:2056	The predicted results	2036:2056	The predicted results	2036:2056	The predicted results were verified by testing these compounds in the same pharmacological assays as for the extracts.
31461974	13	15	theme	pharmacological	2245:2259	arg1	screening					2261:2269	in vitro pharmacological screening	2236:2269	in vitro pharmacological screening	2236:2269	In conclusion, the anti-inflammatory activity of HQJZT could be substantiated by in vitro pharmacological screening, and the predicted activities of the OPLS-DA hits could be partially verified.
31461974	5	16	theme	LC-MS	1127:1131	arg1	techniques					1146:1155	LC-MS metabolomics techniques	1127:1155	LC-MS metabolomics techniques	1127:1155	The present study aimed at identifying constituents contributing to the bioactivity of HQJZT by combining in vitro cytokine production assays and LC-MS metabolomics techniques.
31461974	1	17	theme	chronic	257:263	arg1	conditions					278:287	chronic inflammatory conditions	257:287	chronic inflammatory conditions	257:287	Within non-communicable diseases, chronic inflammatory conditions represent one of the biggest challenges for modern medicine.
31461974	0	18	from	Cytokines	75:83	arg1	Cells					105:109	LPS-Induced U937 Cells	88:109	LPS-Induced U937 Cells	88:109	Identification of Constituents Affecting the Secretion of Pro-Inflammatory Cytokines in LPS-Induced U937 Cells by UHPLC-HRMS-Based Metabolic Profiling of the Traditional Chinese Medicine Formulation Huangqi Jianzhong Tang.
31461974	0	19	theme	Chinese	170:176	arg1	Medicine					178:185	the Traditional Chinese Medicine Formulation Huangqi Jianzhong Tang	154:220	the Traditional Chinese Medicine Formulation Huangqi Jianzhong Tang	154:220	Identification of Constituents Affecting the Secretion of Pro-Inflammatory Cytokines in LPS-Induced U937 Cells by UHPLC-HRMS-Based Metabolic Profiling of the Traditional Chinese Medicine Formulation Huangqi Jianzhong Tang.
31461974	5	20	theme	in	1087:1088	arg1	assays					1116:1121	in vitro cytokine production assays	1087:1121	in vitro cytokine production assays	1087:1121	The present study aimed at identifying constituents contributing to the bioactivity of HQJZT by combining in vitro cytokine production assays and LC-MS metabolomics techniques.
31461974	3	21	used	used	780:783	arg2	Rhizoma					688:694	Rhizoma	688:694	Rhizoma	688:694	Huangqi Jianzhong Tang (HQJZT) is a famous TCM herbal formula composed of Radix Astragali, Ramulus Cinnamomi, Radix et Rhizoma Glycyrrhizae Praeparata cum Melle, Radix Paeoniae Alba, Rhizoma Zingiberis Recens, Fructus Jujubae and Saccharum Granorum (maltose), which has been used for the treatment of various chronic inflammatory gastrointestinal diseases.
31461974	3	21	used	used	780:783	arg2	Astragali					585:593	Astragali	585:593	Astragali	585:593	Huangqi Jianzhong Tang (HQJZT) is a famous TCM herbal formula composed of Radix Astragali, Ramulus Cinnamomi, Radix et Rhizoma Glycyrrhizae Praeparata cum Melle, Radix Paeoniae Alba, Rhizoma Zingiberis Recens, Fructus Jujubae and Saccharum Granorum (maltose), which has been used for the treatment of various chronic inflammatory gastrointestinal diseases.
31461974	3	21	used	used	780:783	arg2	Granorum					745:752	Granorum	745:752	Granorum	745:752	Huangqi Jianzhong Tang (HQJZT) is a famous TCM herbal formula composed of Radix Astragali, Ramulus Cinnamomi, Radix et Rhizoma Glycyrrhizae Praeparata cum Melle, Radix Paeoniae Alba, Rhizoma Zingiberis Recens, Fructus Jujubae and Saccharum Granorum (maltose), which has been used for the treatment of various chronic inflammatory gastrointestinal diseases.
31461974	3	21	used	used	780:783	arg2	Radix					615:619	Radix	615:619	Radix	615:619	Huangqi Jianzhong Tang (HQJZT) is a famous TCM herbal formula composed of Radix Astragali, Ramulus Cinnamomi, Radix et Rhizoma Glycyrrhizae Praeparata cum Melle, Radix Paeoniae Alba, Rhizoma Zingiberis Recens, Fructus Jujubae and Saccharum Granorum (maltose), which has been used for the treatment of various chronic inflammatory gastrointestinal diseases.
31461974	3	21	used	used	780:783	arg2	maltose					755:761	maltose	755:761	maltose	755:761	Huangqi Jianzhong Tang (HQJZT) is a famous TCM herbal formula composed of Radix Astragali, Ramulus Cinnamomi, Radix et Rhizoma Glycyrrhizae Praeparata cum Melle, Radix Paeoniae Alba, Rhizoma Zingiberis Recens, Fructus Jujubae and Saccharum Granorum (maltose), which has been used for the treatment of various chronic inflammatory gastrointestinal diseases.
31461974	3	21	used	used	780:783	arg2	Cinnamomi					604:612	Cinnamomi	604:612	Cinnamomi	604:612	Huangqi Jianzhong Tang (HQJZT) is a famous TCM herbal formula composed of Radix Astragali, Ramulus Cinnamomi, Radix et Rhizoma Glycyrrhizae Praeparata cum Melle, Radix Paeoniae Alba, Rhizoma Zingiberis Recens, Fructus Jujubae and Saccharum Granorum (maltose), which has been used for the treatment of various chronic inflammatory gastrointestinal diseases.
31461974	3	21	used	used	780:783	arg2	Jujubae					723:729	Jujubae	723:729	Jujubae	723:729	Huangqi Jianzhong Tang (HQJZT) is a famous TCM herbal formula composed of Radix Astragali, Ramulus Cinnamomi, Radix et Rhizoma Glycyrrhizae Praeparata cum Melle, Radix Paeoniae Alba, Rhizoma Zingiberis Recens, Fructus Jujubae and Saccharum Granorum (maltose), which has been used for the treatment of various chronic inflammatory gastrointestinal diseases.
31461974	8	22	theme	inhibitory	1365:1374	arg1	effects					1376:1382	The inhibitory effects	1361:1382	The inhibitory effects of the extracts on TNF-α, IL-1β and IFN-γ production	1361:1435	The inhibitory effects of the extracts on TNF-α, IL-1β and IFN-γ production were studied in U937 cells.
31461974	3	23	theme	chronic	814:820	arg1	diseases					852:859	various chronic inflammatory gastrointestinal diseases	806:859	various chronic inflammatory gastrointestinal diseases	806:859	Huangqi Jianzhong Tang (HQJZT) is a famous TCM herbal formula composed of Radix Astragali, Ramulus Cinnamomi, Radix et Rhizoma Glycyrrhizae Praeparata cum Melle, Radix Paeoniae Alba, Rhizoma Zingiberis Recens, Fructus Jujubae and Saccharum Granorum (maltose), which has been used for the treatment of various chronic inflammatory gastrointestinal diseases.
31461974	13	24	theme	HQJZT	2204:2208	arg1	activity					2192:2199	the anti-inflammatory activity	2170:2199	the anti-inflammatory activity of HQJZT	2170:2208	In conclusion, the anti-inflammatory activity of HQJZT could be substantiated by in vitro pharmacological screening, and the predicted activities of the OPLS-DA hits could be partially verified.
31461974	0	25	dep	Medicine	178:185	arg1	Tang					217:220	Tang	217:220	Tang	217:220	Identification of Constituents Affecting the Secretion of Pro-Inflammatory Cytokines in LPS-Induced U937 Cells by UHPLC-HRMS-Based Metabolic Profiling of the Traditional Chinese Medicine Formulation Huangqi Jianzhong Tang.
31461974	0	26	from	Cells	105:109	arg1	Secretion					45:53	the Secretion	41:53	the Secretion of Pro-Inflammatory Cytokines in LPS-Induced U937 Cells by UHPLC-HRMS-Based Metabolic Profiling of the Traditional Chinese Medicine Formulation Huangqi Jianzhong Tang	41:220	Identification of Constituents Affecting the Secretion of Pro-Inflammatory Cytokines in LPS-Induced U937 Cells by UHPLC-HRMS-Based Metabolic Profiling of the Traditional Chinese Medicine Formulation Huangqi Jianzhong Tang.
31461974	6	27	theme	different	1241:1249	arg1	polarities					1251:1260	different polarities	1241:1260	different polarities	1241:1260	From the HQJZT decoction as well as from its single herbal components, extracts of different polarities were prepared.
31461974	14	28	theme	active	2430:2435	arg1	compounds					2437:2445	MVDA for prediction pharmacologically active compounds	2392:2445	MVDA for prediction pharmacologically active compounds	2392:2445	Moreover, the benefits and limitations of MVDA for prediction pharmacologically active compounds contributing to the activity of a TCM mixture could be detected.
31461974	0	29	theme	Cytokines	75:83	arg1	Secretion					45:53	the Secretion	41:53	the Secretion of Pro-Inflammatory Cytokines in LPS-Induced U937 Cells by UHPLC-HRMS-Based Metabolic Profiling of the Traditional Chinese Medicine Formulation Huangqi Jianzhong Tang	41:220	Identification of Constituents Affecting the Secretion of Pro-Inflammatory Cytokines in LPS-Induced U937 Cells by UHPLC-HRMS-Based Metabolic Profiling of the Traditional Chinese Medicine Formulation Huangqi Jianzhong Tang.
31461974	8	30	theme	extracts	1391:1398	arg1	effects					1376:1382	The inhibitory effects	1361:1382	The inhibitory effects of the extracts on TNF-α, IL-1β and IFN-γ production	1361:1435	The inhibitory effects of the extracts on TNF-α, IL-1β and IFN-γ production were studied in U937 cells.
31461974	0	31	theme	LPS-Induced	88:98	arg1	Cells					105:109	LPS-Induced U937 Cells	88:109	LPS-Induced U937 Cells	88:109	Identification of Constituents Affecting the Secretion of Pro-Inflammatory Cytokines in LPS-Induced U937 Cells by UHPLC-HRMS-Based Metabolic Profiling of the Traditional Chinese Medicine Formulation Huangqi Jianzhong Tang.
31461974	5	32	theme	cytokine	1096:1103	arg1	production					1105:1114	cytokine production	1096:1114	in vitro cytokine production assays	1087:1121	The present study aimed at identifying constituents contributing to the bioactivity of HQJZT by combining in vitro cytokine production assays and LC-MS metabolomics techniques.
31461974	14	33	theme	MVDA	2392:2395	arg1	compounds					2437:2445	MVDA for prediction pharmacologically active compounds	2392:2445	MVDA for prediction pharmacologically active compounds	2392:2445	Moreover, the benefits and limitations of MVDA for prediction pharmacologically active compounds contributing to the activity of a TCM mixture could be detected.
31461974	3	34	theme	gastrointestinal	835:850	arg1	diseases					852:859	various chronic inflammatory gastrointestinal diseases	806:859	various chronic inflammatory gastrointestinal diseases	806:859	Huangqi Jianzhong Tang (HQJZT) is a famous TCM herbal formula composed of Radix Astragali, Ramulus Cinnamomi, Radix et Rhizoma Glycyrrhizae Praeparata cum Melle, Radix Paeoniae Alba, Rhizoma Zingiberis Recens, Fructus Jujubae and Saccharum Granorum (maltose), which has been used for the treatment of various chronic inflammatory gastrointestinal diseases.
31461974	10	35	located	observed	1848:1855	arg1	cells					1865:1869	U937 cells	1860:1869	U937 cells	1860:1869	The investigations resulted in the identification of 16 HQJZT constituents, which are likely to contribute to the activities observed in U937 cells.
31461974	10	35	located	observed	1848:1855	arg2	activities					1837:1846	the activities	1833:1846	the activities observed in U937 cells	1833:1869	The investigations resulted in the identification of 16 HQJZT constituents, which are likely to contribute to the activities observed in U937 cells.
31461974	2	36	theme	empirical	450:458	arg1	knowledge					460:468	tremendous empirical knowledge	439:468	tremendous empirical knowledge	439:468	Traditional Chinese Medicine (TCM) has been practiced over centuries and has accumulated tremendous empirical knowledge on the treatment of such diseases.
31461974	9	37	theme	pharmacological	1483:1497	arg1	data					1511:1514	Phytochemical and pharmacological bioactivity data	1465:1514	Phytochemical and pharmacological bioactivity data	1465:1514	Phytochemical and pharmacological bioactivity data were correlated by orthogonal projection to latent structures discriminant analysis (OPLS-DA) in order to identify those HQJZT constituents which may be relevant for the observed pharmacological activities.
31461974	8	38	theme	IFN-γ	1420:1424	arg1	production					1426:1435	IFN-γ production	1420:1435	IFN-γ production	1420:1435	The inhibitory effects of the extracts on TNF-α, IL-1β and IFN-γ production were studied in U937 cells.
31461974	2	39	theme	such	490:493	arg1	diseases					495:502	such diseases	490:502	such diseases	490:502	Traditional Chinese Medicine (TCM) has been practiced over centuries and has accumulated tremendous empirical knowledge on the treatment of such diseases.
31461974	0	40	theme	Constituents	18:29	arg1	Identification					0:13	Identification	0:13	Identification of Constituents Affecting the Secretion of Pro-Inflammatory Cytokines in LPS-Induced U937 Cells by UHPLC-HRMS-Based Metabolic Profiling of the Traditional Chinese Medicine Formulation Huangqi Jianzhong Tang.	0:221	Identification of Constituents Affecting the Secretion of Pro-Inflammatory Cytokines in LPS-Induced U937 Cells by UHPLC-HRMS-Based Metabolic Profiling of the Traditional Chinese Medicine Formulation Huangqi Jianzhong Tang.
31461974	9	41	theme	bioactivity	1499:1509	arg1	data					1511:1514	Phytochemical and pharmacological bioactivity data	1465:1514	Phytochemical and pharmacological bioactivity data	1465:1514	Phytochemical and pharmacological bioactivity data were correlated by orthogonal projection to latent structures discriminant analysis (OPLS-DA) in order to identify those HQJZT constituents which may be relevant for the observed pharmacological activities.
31461974	5	42	theme	HQJZT	1068:1072	arg1	bioactivity					1053:1063	the bioactivity	1049:1063	the bioactivity of HQJZT	1049:1072	The present study aimed at identifying constituents contributing to the bioactivity of HQJZT by combining in vitro cytokine production assays and LC-MS metabolomics techniques.
31461974	13	43	theme	OPLS-DA	2308:2314	arg1	hits					2316:2319	the OPLS-DA hits	2304:2319	the OPLS-DA hits	2304:2319	In conclusion, the anti-inflammatory activity of HQJZT could be substantiated by in vitro pharmacological screening, and the predicted activities of the OPLS-DA hits could be partially verified.
31461974	10	44	theme	constituents	1785:1796	arg1	identification					1758:1771	the identification	1754:1771	the identification of 16 HQJZT constituents, which are likely to contribute to the activities observed in U937 cells	1754:1869	The investigations resulted in the identification of 16 HQJZT constituents, which are likely to contribute to the activities observed in U937 cells.
31461974	1	45	theme	inflammatory	265:276	arg1	conditions					278:287	chronic inflammatory conditions	257:287	chronic inflammatory conditions	257:287	Within non-communicable diseases, chronic inflammatory conditions represent one of the biggest challenges for modern medicine.
31461974	9	46	theme	pharmacological	1695:1709	arg1	activities					1711:1720	the observed pharmacological activities	1682:1720	the observed pharmacological activities	1682:1720	Phytochemical and pharmacological bioactivity data were correlated by orthogonal projection to latent structures discriminant analysis (OPLS-DA) in order to identify those HQJZT constituents which may be relevant for the observed pharmacological activities.
31461974	6	47	theme	HQJZT	1167:1171	arg1	decoction					1173:1181	the HQJZT decoction	1163:1181	the HQJZT decoction	1163:1181	From the HQJZT decoction as well as from its single herbal components, extracts of different polarities were prepared.
31461974	13	48	dep	in	2236:2237	arg1	vitro					2239:2243	vitro	2239:2243	vitro	2239:2243	In conclusion, the anti-inflammatory activity of HQJZT could be substantiated by in vitro pharmacological screening, and the predicted activities of the OPLS-DA hits could be partially verified.
31461974	11	49	theme	18β-glycyrrhetinic	1952:1969	arg1	them					1881:1884	them	1881:1884	them	1881:1884	Seven of them, namely calycosin, formononetin, astragaloside I, liquiritigenin, 18β-glycyrrhetinic acid, paeoniflorin and albiflorin were unambiguously identified.
31461974	11	49	theme	18β-glycyrrhetinic	1952:1969	arg1	Seven					1872:1876	Seven	1872:1876	Seven	1872:1876	Seven of them, namely calycosin, formononetin, astragaloside I, liquiritigenin, 18β-glycyrrhetinic acid, paeoniflorin and albiflorin were unambiguously identified.
31461974	11	49	theme	18β-glycyrrhetinic	1952:1969	arg1	acid					1971:1974	18β-glycyrrhetinic acid	1952:1974	18β-glycyrrhetinic acid	1952:1974	Seven of them, namely calycosin, formononetin, astragaloside I, liquiritigenin, 18β-glycyrrhetinic acid, paeoniflorin and albiflorin were unambiguously identified.
31461974	13	50	theme	predicted	2280:2288	arg1	activities					2290:2299	the predicted activities	2276:2299	the predicted activities of the OPLS-DA hits	2276:2319	In conclusion, the anti-inflammatory activity of HQJZT could be substantiated by in vitro pharmacological screening, and the predicted activities of the OPLS-DA hits could be partially verified.
31461974	12	51	theme	pharmacological	2111:2125	arg1	assays					2127:2132	the same pharmacological assays	2102:2132	the same pharmacological assays as for the extracts	2102:2152	The predicted results were verified by testing these compounds in the same pharmacological assays as for the extracts.
31461974	3	52	theme	famous	541:546	arg1	Tang					523:526	Tang	523:526	Tang	523:526	Huangqi Jianzhong Tang (HQJZT) is a famous TCM herbal formula composed of Radix Astragali, Ramulus Cinnamomi, Radix et Rhizoma Glycyrrhizae Praeparata cum Melle, Radix Paeoniae Alba, Rhizoma Zingiberis Recens, Fructus Jujubae and Saccharum Granorum (maltose), which has been used for the treatment of various chronic inflammatory gastrointestinal diseases.
31461974	3	52	theme	famous	541:546	arg1	formula					559:565	a famous TCM herbal formula	539:565	a famous TCM herbal formula composed of Radix Astragali, Ramulus Cinnamomi, Radix et Rhizoma Glycyrrhizae Praeparata cum Melle, Radix Paeoniae Alba, Rhizoma Zingiberis Recens, Fructus Jujubae and Saccharum Granorum (maltose), which has been used for the treatment of various chronic inflammatory gastrointestinal diseases	539:859	Huangqi Jianzhong Tang (HQJZT) is a famous TCM herbal formula composed of Radix Astragali, Ramulus Cinnamomi, Radix et Rhizoma Glycyrrhizae Praeparata cum Melle, Radix Paeoniae Alba, Rhizoma Zingiberis Recens, Fructus Jujubae and Saccharum Granorum (maltose), which has been used for the treatment of various chronic inflammatory gastrointestinal diseases.
31461974	3	53	dep	Rhizoma	688:694	arg1	Zingiberis					696:705	Rhizoma Zingiberis Recens	688:712	Rhizoma Zingiberis Recens	688:712	Huangqi Jianzhong Tang (HQJZT) is a famous TCM herbal formula composed of Radix Astragali, Ramulus Cinnamomi, Radix et Rhizoma Glycyrrhizae Praeparata cum Melle, Radix Paeoniae Alba, Rhizoma Zingiberis Recens, Fructus Jujubae and Saccharum Granorum (maltose), which has been used for the treatment of various chronic inflammatory gastrointestinal diseases.
31461974	3	53	dep	Rhizoma	688:694	arg1	Recens					707:712	Recens	707:712	Rhizoma Zingiberis Recens	688:712	Huangqi Jianzhong Tang (HQJZT) is a famous TCM herbal formula composed of Radix Astragali, Ramulus Cinnamomi, Radix et Rhizoma Glycyrrhizae Praeparata cum Melle, Radix Paeoniae Alba, Rhizoma Zingiberis Recens, Fructus Jujubae and Saccharum Granorum (maltose), which has been used for the treatment of various chronic inflammatory gastrointestinal diseases.
31461974	1	54	theme	biggest	310:316	arg1	challenges					318:327	the biggest challenges	306:327	the biggest challenges for modern medicine	306:347	Within non-communicable diseases, chronic inflammatory conditions represent one of the biggest challenges for modern medicine.
31461974	14	55	theme	TCM	2481:2483	arg1	mixture					2485:2491	a TCM mixture	2479:2491	a TCM mixture	2479:2491	Moreover, the benefits and limitations of MVDA for prediction pharmacologically active compounds contributing to the activity of a TCM mixture could be detected.
31461974	3	56	dep	Radix	615:619	arg1	Radix					667:671	Radix	667:671	Radix	667:671	Huangqi Jianzhong Tang (HQJZT) is a famous TCM herbal formula composed of Radix Astragali, Ramulus Cinnamomi, Radix et Rhizoma Glycyrrhizae Praeparata cum Melle, Radix Paeoniae Alba, Rhizoma Zingiberis Recens, Fructus Jujubae and Saccharum Granorum (maltose), which has been used for the treatment of various chronic inflammatory gastrointestinal diseases.
31461974	3	56	dep	Radix	615:619	arg1	et					621:622	Radix et Rhizoma Glycyrrhizae Praeparata cum Melle, Radix Paeoniae Alba	615:685	Radix et Rhizoma Glycyrrhizae Praeparata cum Melle, Radix Paeoniae Alba	615:685	Huangqi Jianzhong Tang (HQJZT) is a famous TCM herbal formula composed of Radix Astragali, Ramulus Cinnamomi, Radix et Rhizoma Glycyrrhizae Praeparata cum Melle, Radix Paeoniae Alba, Rhizoma Zingiberis Recens, Fructus Jujubae and Saccharum Granorum (maltose), which has been used for the treatment of various chronic inflammatory gastrointestinal diseases.
31461974	3	56	dep	Radix	615:619	arg1	Paeoniae					673:680	Paeoniae	673:680	Paeoniae	673:680	Huangqi Jianzhong Tang (HQJZT) is a famous TCM herbal formula composed of Radix Astragali, Ramulus Cinnamomi, Radix et Rhizoma Glycyrrhizae Praeparata cum Melle, Radix Paeoniae Alba, Rhizoma Zingiberis Recens, Fructus Jujubae and Saccharum Granorum (maltose), which has been used for the treatment of various chronic inflammatory gastrointestinal diseases.
31461974	6	57	theme	herbal	1210:1215	arg1	components					1217:1226	its single herbal components	1199:1226	its single herbal components	1199:1226	From the HQJZT decoction as well as from its single herbal components, extracts of different polarities were prepared.
31461974	2	58	theme	Chinese	362:368	arg1	TCM					380:382	TCM	380:382	TCM	380:382	Traditional Chinese Medicine (TCM) has been practiced over centuries and has accumulated tremendous empirical knowledge on the treatment of such diseases.
31461974	2	58	theme	Chinese	362:368	arg1	Medicine					370:377	Traditional Chinese Medicine	350:377	Traditional Chinese Medicine (TCM)	350:383	Traditional Chinese Medicine (TCM) has been practiced over centuries and has accumulated tremendous empirical knowledge on the treatment of such diseases.
31461974	1	59	theme	non-communicable	230:245	arg1	diseases					247:254	non-communicable diseases	230:254	non-communicable diseases	230:254	Within non-communicable diseases, chronic inflammatory conditions represent one of the biggest challenges for modern medicine.
31461974	4	60	theme	responsible	952:962	arg1	mechanisms					941:950	the mechanisms	937:950	the mechanisms responsible for its effects	937:978	However, there is insufficient knowledge about its active constituents and the mechanisms responsible for its effects.
31461974	0	61	theme	Traditional	158:168	arg1	Medicine					178:185	the Traditional Chinese Medicine Formulation Huangqi Jianzhong Tang	154:220	the Traditional Chinese Medicine Formulation Huangqi Jianzhong Tang	154:220	Identification of Constituents Affecting the Secretion of Pro-Inflammatory Cytokines in LPS-Induced U937 Cells by UHPLC-HRMS-Based Metabolic Profiling of the Traditional Chinese Medicine Formulation Huangqi Jianzhong Tang.
31461974	9	62	theme	projection	1546:1555	arg1	OPLS-DA					1601:1607	OPLS-DA	1601:1607	OPLS-DA	1601:1607	Phytochemical and pharmacological bioactivity data were correlated by orthogonal projection to latent structures discriminant analysis (OPLS-DA) in order to identify those HQJZT constituents which may be relevant for the observed pharmacological activities.
31461974	9	62	theme	projection	1546:1555	arg1	analysis					1591:1598	orthogonal projection to latent structures discriminant analysis	1535:1598	orthogonal projection to latent structures discriminant analysis (OPLS-DA)	1535:1608	Phytochemical and pharmacological bioactivity data were correlated by orthogonal projection to latent structures discriminant analysis (OPLS-DA) in order to identify those HQJZT constituents which may be relevant for the observed pharmacological activities.
31461974	0	63	theme	Medicine	178:185	arg1	Profiling					141:149	UHPLC-HRMS-Based Metabolic Profiling	114:149	UHPLC-HRMS-Based Metabolic Profiling of the Traditional Chinese Medicine Formulation Huangqi Jianzhong Tang	114:220	Identification of Constituents Affecting the Secretion of Pro-Inflammatory Cytokines in LPS-Induced U937 Cells by UHPLC-HRMS-Based Metabolic Profiling of the Traditional Chinese Medicine Formulation Huangqi Jianzhong Tang.
31461974	13	64	theme	in	2236:2237	arg1	screening					2261:2269	in vitro pharmacological screening	2236:2269	in vitro pharmacological screening	2236:2269	In conclusion, the anti-inflammatory activity of HQJZT could be substantiated by in vitro pharmacological screening, and the predicted activities of the OPLS-DA hits could be partially verified.
31461974	9	65	theme	latent	1560:1565	arg1	structures					1567:1576	latent structures	1560:1576	latent structures	1560:1576	Phytochemical and pharmacological bioactivity data were correlated by orthogonal projection to latent structures discriminant analysis (OPLS-DA) in order to identify those HQJZT constituents which may be relevant for the observed pharmacological activities.
31461974	9	66	theme	discriminant	1578:1589	arg1	OPLS-DA					1601:1607	OPLS-DA	1601:1607	OPLS-DA	1601:1607	Phytochemical and pharmacological bioactivity data were correlated by orthogonal projection to latent structures discriminant analysis (OPLS-DA) in order to identify those HQJZT constituents which may be relevant for the observed pharmacological activities.
31461974	9	66	theme	discriminant	1578:1589	arg1	analysis					1591:1598	orthogonal projection to latent structures discriminant analysis	1535:1598	orthogonal projection to latent structures discriminant analysis (OPLS-DA)	1535:1608	Phytochemical and pharmacological bioactivity data were correlated by orthogonal projection to latent structures discriminant analysis (OPLS-DA) in order to identify those HQJZT constituents which may be relevant for the observed pharmacological activities.
31461974	10	67	theme	U937	1860:1863	arg1	cells					1865:1869	U937 cells	1860:1869	U937 cells	1860:1869	The investigations resulted in the identification of 16 HQJZT constituents, which are likely to contribute to the activities observed in U937 cells.
31461974	6	68	theme	polarities	1251:1260	arg1	extracts					1229:1236	extracts	1229:1236	extracts of different polarities	1229:1260	From the HQJZT decoction as well as from its single herbal components, extracts of different polarities were prepared.
31461974	14	69	theme	compounds	2437:2445	arg1	limitations					2377:2387	limitations	2377:2387	limitations	2377:2387	Moreover, the benefits and limitations of MVDA for prediction pharmacologically active compounds contributing to the activity of a TCM mixture could be detected.
31461974	14	69	theme	compounds	2437:2445	arg1	benefits					2364:2371	benefits	2364:2371	benefits	2364:2371	Moreover, the benefits and limitations of MVDA for prediction pharmacologically active compounds contributing to the activity of a TCM mixture could be detected.
31461974	3	70	theme	inflammatory	822:833	arg1	diseases					852:859	various chronic inflammatory gastrointestinal diseases	806:859	various chronic inflammatory gastrointestinal diseases	806:859	Huangqi Jianzhong Tang (HQJZT) is a famous TCM herbal formula composed of Radix Astragali, Ramulus Cinnamomi, Radix et Rhizoma Glycyrrhizae Praeparata cum Melle, Radix Paeoniae Alba, Rhizoma Zingiberis Recens, Fructus Jujubae and Saccharum Granorum (maltose), which has been used for the treatment of various chronic inflammatory gastrointestinal diseases.
31461974	7	71	theme	Phytochemical	1277:1289	arg1	composition					1291:1301	Phytochemical composition	1277:1301	Phytochemical composition of the extracts	1277:1317	Phytochemical composition of the extracts was analyzed by means of UPLC-QTOF-MS/MS.
31461974	5	72	theme	production	1105:1114	arg1	assays					1116:1121	in vitro cytokine production assays	1087:1121	in vitro cytokine production assays	1087:1121	The present study aimed at identifying constituents contributing to the bioactivity of HQJZT by combining in vitro cytokine production assays and LC-MS metabolomics techniques.
31461974	3	73	theme	various	806:812	arg1	diseases					852:859	various chronic inflammatory gastrointestinal diseases	806:859	various chronic inflammatory gastrointestinal diseases	806:859	Huangqi Jianzhong Tang (HQJZT) is a famous TCM herbal formula composed of Radix Astragali, Ramulus Cinnamomi, Radix et Rhizoma Glycyrrhizae Praeparata cum Melle, Radix Paeoniae Alba, Rhizoma Zingiberis Recens, Fructus Jujubae and Saccharum Granorum (maltose), which has been used for the treatment of various chronic inflammatory gastrointestinal diseases.
31461974	0	74	theme	U937	100:103	arg1	Cells					105:109	LPS-Induced U937 Cells	88:109	LPS-Induced U937 Cells	88:109	Identification of Constituents Affecting the Secretion of Pro-Inflammatory Cytokines in LPS-Induced U937 Cells by UHPLC-HRMS-Based Metabolic Profiling of the Traditional Chinese Medicine Formulation Huangqi Jianzhong Tang.
31461974	13	75	theme	anti-inflammatory	2174:2190	arg1	activity					2192:2199	the anti-inflammatory activity	2170:2199	the anti-inflammatory activity of HQJZT	2170:2208	In conclusion, the anti-inflammatory activity of HQJZT could be substantiated by in vitro pharmacological screening, and the predicted activities of the OPLS-DA hits could be partially verified.
31461974	10	76	theme	HQJZT	1779:1783	arg1	constituents					1785:1796	16 HQJZT constituents	1776:1796	16 HQJZT constituents	1776:1796	The investigations resulted in the identification of 16 HQJZT constituents, which are likely to contribute to the activities observed in U937 cells.
31461974	0	77	theme	Metabolic	131:139	arg1	Profiling					141:149	UHPLC-HRMS-Based Metabolic Profiling	114:149	UHPLC-HRMS-Based Metabolic Profiling of the Traditional Chinese Medicine Formulation Huangqi Jianzhong Tang	114:220	Identification of Constituents Affecting the Secretion of Pro-Inflammatory Cytokines in LPS-Induced U937 Cells by UHPLC-HRMS-Based Metabolic Profiling of the Traditional Chinese Medicine Formulation Huangqi Jianzhong Tang.
31461974	3	78	theme	diseases	852:859	arg1	treatment					793:801	the treatment	789:801	the treatment of various chronic inflammatory gastrointestinal diseases	789:859	Huangqi Jianzhong Tang (HQJZT) is a famous TCM herbal formula composed of Radix Astragali, Ramulus Cinnamomi, Radix et Rhizoma Glycyrrhizae Praeparata cum Melle, Radix Paeoniae Alba, Rhizoma Zingiberis Recens, Fructus Jujubae and Saccharum Granorum (maltose), which has been used for the treatment of various chronic inflammatory gastrointestinal diseases.
31461974	0	79	from	Secretion	45:53	arg1	Cells					105:109	LPS-Induced U937 Cells	88:109	LPS-Induced U937 Cells	88:109	Identification of Constituents Affecting the Secretion of Pro-Inflammatory Cytokines in LPS-Induced U937 Cells by UHPLC-HRMS-Based Metabolic Profiling of the Traditional Chinese Medicine Formulation Huangqi Jianzhong Tang.
31461974	2	80	theme	tremendous	439:448	arg1	knowledge					460:468	tremendous empirical knowledge	439:468	tremendous empirical knowledge	439:468	Traditional Chinese Medicine (TCM) has been practiced over centuries and has accumulated tremendous empirical knowledge on the treatment of such diseases.
31461974	9	81	theme	HQJZT	1637:1641	arg1	constituents					1643:1654	those HQJZT constituents	1631:1654	those HQJZT constituents which may be relevant for the observed pharmacological activities	1631:1720	Phytochemical and pharmacological bioactivity data were correlated by orthogonal projection to latent structures discriminant analysis (OPLS-DA) in order to identify those HQJZT constituents which may be relevant for the observed pharmacological activities.
31461974	2	82	theme	diseases	495:502	arg1	treatment					477:485	the treatment	473:485	the treatment of such diseases	473:502	Traditional Chinese Medicine (TCM) has been practiced over centuries and has accumulated tremendous empirical knowledge on the treatment of such diseases.
31461974	1	83	theme	modern	333:338	arg1	medicine					340:347	modern medicine	333:347	modern medicine	333:347	Within non-communicable diseases, chronic inflammatory conditions represent one of the biggest challenges for modern medicine.
31461974	8	84	from	effects	1376:1382	arg1	IL-1β					1410:1414	IL-1β	1410:1414	IL-1β	1410:1414	The inhibitory effects of the extracts on TNF-α, IL-1β and IFN-γ production were studied in U937 cells.
31461974	8	84	from	effects	1376:1382	arg1	production					1426:1435	IFN-γ production	1420:1435	IFN-γ production	1420:1435	The inhibitory effects of the extracts on TNF-α, IL-1β and IFN-γ production were studied in U937 cells.
31461974	8	84	from	effects	1376:1382	arg1	TNF-α					1403:1407	TNF-α	1403:1407	TNF-α	1403:1407	The inhibitory effects of the extracts on TNF-α, IL-1β and IFN-γ production were studied in U937 cells.
31461974	5	85	theme	present	985:991	arg1	study					993:997	The present study	981:997	The present study	981:997	The present study aimed at identifying constituents contributing to the bioactivity of HQJZT by combining in vitro cytokine production assays and LC-MS metabolomics techniques.
31461974	3	86	theme	herbal	552:557	arg1	Tang					523:526	Tang	523:526	Tang	523:526	Huangqi Jianzhong Tang (HQJZT) is a famous TCM herbal formula composed of Radix Astragali, Ramulus Cinnamomi, Radix et Rhizoma Glycyrrhizae Praeparata cum Melle, Radix Paeoniae Alba, Rhizoma Zingiberis Recens, Fructus Jujubae and Saccharum Granorum (maltose), which has been used for the treatment of various chronic inflammatory gastrointestinal diseases.
31461974	3	86	theme	herbal	552:557	arg1	formula					559:565	a famous TCM herbal formula	539:565	a famous TCM herbal formula composed of Radix Astragali, Ramulus Cinnamomi, Radix et Rhizoma Glycyrrhizae Praeparata cum Melle, Radix Paeoniae Alba, Rhizoma Zingiberis Recens, Fructus Jujubae and Saccharum Granorum (maltose), which has been used for the treatment of various chronic inflammatory gastrointestinal diseases	539:859	Huangqi Jianzhong Tang (HQJZT) is a famous TCM herbal formula composed of Radix Astragali, Ramulus Cinnamomi, Radix et Rhizoma Glycyrrhizae Praeparata cum Melle, Radix Paeoniae Alba, Rhizoma Zingiberis Recens, Fructus Jujubae and Saccharum Granorum (maltose), which has been used for the treatment of various chronic inflammatory gastrointestinal diseases.
31461974	0	87	theme	UHPLC-HRMS-Based	114:129	arg1	Profiling					141:149	UHPLC-HRMS-Based Metabolic Profiling	114:149	UHPLC-HRMS-Based Metabolic Profiling of the Traditional Chinese Medicine Formulation Huangqi Jianzhong Tang	114:220	Identification of Constituents Affecting the Secretion of Pro-Inflammatory Cytokines in LPS-Induced U937 Cells by UHPLC-HRMS-Based Metabolic Profiling of the Traditional Chinese Medicine Formulation Huangqi Jianzhong Tang.
31461974	0	88	theme	Pro-Inflammatory	58:73	arg1	Cytokines					75:83	Pro-Inflammatory Cytokines	58:83	Pro-Inflammatory Cytokines in LPS-Induced U937 Cells	58:109	Identification of Constituents Affecting the Secretion of Pro-Inflammatory Cytokines in LPS-Induced U937 Cells by UHPLC-HRMS-Based Metabolic Profiling of the Traditional Chinese Medicine Formulation Huangqi Jianzhong Tang.
31461974	8	89	theme	U937	1453:1456	arg1	cells					1458:1462	U937 cells	1453:1462	U937 cells	1453:1462	The inhibitory effects of the extracts on TNF-α, IL-1β and IFN-γ production were studied in U937 cells.
31461974	13	90	theme	hits	2316:2319	arg1	activities					2290:2299	the predicted activities	2276:2299	the predicted activities of the OPLS-DA hits	2276:2319	In conclusion, the anti-inflammatory activity of HQJZT could be substantiated by in vitro pharmacological screening, and the predicted activities of the OPLS-DA hits could be partially verified.
31461974	9	91	theme	observed	1686:1693	arg1	activities					1711:1720	the observed pharmacological activities	1682:1720	the observed pharmacological activities	1682:1720	Phytochemical and pharmacological bioactivity data were correlated by orthogonal projection to latent structures discriminant analysis (OPLS-DA) in order to identify those HQJZT constituents which may be relevant for the observed pharmacological activities.
31461974	11	92	theme	astragaloside	1919:1931	arg1	I					1933:1933	astragaloside I	1919:1933	astragaloside I	1919:1933	Seven of them, namely calycosin, formononetin, astragaloside I, liquiritigenin, 18β-glycyrrhetinic acid, paeoniflorin and albiflorin were unambiguously identified.
31461974	11	92	theme	astragaloside	1919:1931	arg1	them					1881:1884	them	1881:1884	them	1881:1884	Seven of them, namely calycosin, formononetin, astragaloside I, liquiritigenin, 18β-glycyrrhetinic acid, paeoniflorin and albiflorin were unambiguously identified.
31461974	11	92	theme	astragaloside	1919:1931	arg1	Seven					1872:1876	Seven	1872:1876	Seven	1872:1876	Seven of them, namely calycosin, formononetin, astragaloside I, liquiritigenin, 18β-glycyrrhetinic acid, paeoniflorin and albiflorin were unambiguously identified.
31461974	3	93	dep	Paeoniae	673:680	arg1	Alba					682:685	Rhizoma Glycyrrhizae Praeparata cum Melle, Radix Paeoniae Alba	624:685	Alba	682:685	Huangqi Jianzhong Tang (HQJZT) is a famous TCM herbal formula composed of Radix Astragali, Ramulus Cinnamomi, Radix et Rhizoma Glycyrrhizae Praeparata cum Melle, Radix Paeoniae Alba, Rhizoma Zingiberis Recens, Fructus Jujubae and Saccharum Granorum (maltose), which has been used for the treatment of various chronic inflammatory gastrointestinal diseases.
31461974	7	94	theme	extracts	1310:1317	arg1	composition					1291:1301	Phytochemical composition	1277:1301	Phytochemical composition of the extracts	1277:1317	Phytochemical composition of the extracts was analyzed by means of UPLC-QTOF-MS/MS.
29605718	7	0	theme	glycosaminoglycan-rich	1192:1213	arg1	matrix					1218:1223	glycosaminoglycan-rich NP matrix	1192:1223	glycosaminoglycan-rich NP matrix	1192:1223	Polarized microscopy and FTIR spectroscopy demonstrated replacement of glycosaminoglycan-rich NP matrix with collagenous fibrous tissue.
29605718	3	1	theme	"	685:685	arg1	mice					692:695	"super-healer" LG/J mice	672:695	"super-healer" LG/J mice	672:695	We report, for the first time, early onset spontaneous disc degeneration in SM/J mice known for their poor regenerative capacities compared to "super-healer" LG/J mice.
29605718	10	2	theme	dystrophic	1626:1635	arg1	mineralization					1637:1650	dystrophic mineralization	1626:1650	dystrophic mineralization	1626:1650	Likewise, expression of Enpp1 as well as Alpl was higher, suggesting NP cells of SM/J mice promote dystrophic mineralization.
29605718	12	3	theme	mechanical	1908:1917	arg1	functionality					1919:1931	compromised motion segment mechanical functionality	1881:1931	compromised motion segment mechanical functionality	1881:1931	Importantly, SM/J discs were stiffer, had decreased height, and poor vertebral bone quality, suggesting compromised motion segment mechanical functionality.
29605718	3	4	theme	first	548:552	arg1	time					554:557	the first time	544:557	the first time	544:557	We report, for the first time, early onset spontaneous disc degeneration in SM/J mice known for their poor regenerative capacities compared to "super-healer" LG/J mice.
29605718	6	5	theme	marker	1102:1107	arg1	expression					1109:1118	decreased phenotypic marker expression	1081:1118	decreased phenotypic marker expression	1081:1118	There was increased NP cell death in SM/J mice with decreased phenotypic marker expression.
29605718	9	6	theme	X	1382:1382	arg1	expression					1359:1368	an aberrant expression	1347:1368	an aberrant expression of collagen X and MMP13	1347:1392	Furthermore, an aberrant expression of collagen X and MMP13 was observed in the NP of SM/J mice, along with elevated expression of Col10a1, Ctgf, and Runx2, markers of chondrocyte hypertrophy.
29605718	6	7	theme	decreased	1081:1089	arg1	expression					1109:1118	decreased phenotypic marker expression	1081:1118	decreased phenotypic marker expression	1081:1118	There was increased NP cell death in SM/J mice with decreased phenotypic marker expression.
29605718	11	8	theme	cell	1716:1719	arg1	survival					1721:1728	NP cell survival	1713:1728	NP cell survival	1713:1728	There was also a decrease in several pathways necessary for NP cell survival and function including Wnt and VEGF signaling.
29605718	3	9	theme	onset	566:570	arg1	degeneration					589:600	early onset spontaneous disc degeneration	560:600	early onset spontaneous disc degeneration in SM/J mice known for their poor regenerative capacities compared to "super-healer" LG/J mice	560:695	We report, for the first time, early onset spontaneous disc degeneration in SM/J mice known for their poor regenerative capacities compared to "super-healer" LG/J mice.
29605718	12	10	theme	motion	1893:1898	arg1	functionality					1919:1931	compromised motion segment mechanical functionality	1881:1931	compromised motion segment mechanical functionality	1881:1931	Importantly, SM/J discs were stiffer, had decreased height, and poor vertebral bone quality, suggesting compromised motion segment mechanical functionality.
29605718	13	11	theme	human	2044:2048	arg1	degeneration					2055:2066	human disc degeneration	2044:2066	human disc degeneration	2044:2066	Taken together, our results clearly demonstrate that SM/J mouse strain recapitulates many salient features of human disc degeneration, and serves as a novel small animal model.
29605718	9	12	theme	SM/J	1420:1423	arg1	mice					1425:1428	SM/J mice	1420:1428	SM/J mice	1420:1428	Furthermore, an aberrant expression of collagen X and MMP13 was observed in the NP of SM/J mice, along with elevated expression of Col10a1, Ctgf, and Runx2, markers of chondrocyte hypertrophy.
29605718	12	13	theme	vertebral	1846:1854	arg1	quality					1861:1867	poor vertebral bone quality	1841:1867	poor vertebral bone quality	1841:1867	Importantly, SM/J discs were stiffer, had decreased height, and poor vertebral bone quality, suggesting compromised motion segment mechanical functionality.
29605718	5	14	theme	histological	1008:1019	arg1	grades					1021:1026	higher histological grades	1001:1026	higher histological grades	1001:1026	Distinctions between NP and annulus fibrosus (AF) or endplate cartilage were lost, and NP and AF of SM/J mice showed higher histological grades.
29605718	4	15	theme	increased	852:860	arg1	severity					862:869	increased severity	852:869	increased severity	852:869	In SM/J mice, degenerative process was marked by decreased nucleus pulposus (NP) cellularity and changes in matrix composition at P7, 4, and 8 weeks with increased severity by 17 weeks.
29605718	4	16	theme	SM/J	701:704	arg1	mice					706:709	SM/J mice	701:709	SM/J mice	701:709	In SM/J mice, degenerative process was marked by decreased nucleus pulposus (NP) cellularity and changes in matrix composition at P7, 4, and 8 weeks with increased severity by 17 weeks.
29605718	13	17	theme	many	2019:2022	arg1	features					2032:2039	many salient features	2019:2039	many salient features of human disc degeneration	2019:2066	Taken together, our results clearly demonstrate that SM/J mouse strain recapitulates many salient features of human disc degeneration, and serves as a novel small animal model.
29605718	3	18	theme	disc	584:587	arg1	degeneration					589:600	early onset spontaneous disc degeneration	560:600	early onset spontaneous disc degeneration in SM/J mice known for their poor regenerative capacities compared to "super-healer" LG/J mice	560:695	We report, for the first time, early onset spontaneous disc degeneration in SM/J mice known for their poor regenerative capacities compared to "super-healer" LG/J mice.
29605718	4	19	from	changes	795:801	arg1	composition					813:823	matrix composition	806:823	matrix composition	806:823	In SM/J mice, degenerative process was marked by decreased nucleus pulposus (NP) cellularity and changes in matrix composition at P7, 4, and 8 weeks with increased severity by 17 weeks.
29605718	1	20	theme	disc	119:122	arg1	degeneration					124:135	Intervertebral disc degeneration	104:135	Intervertebral disc degeneration	104:135	Intervertebral disc degeneration and associated low back and neck pain is a ubiquitous health condition that affects millions of people world-wide, and causes high incidence of disability and enormous medical/societal costs.
29605718	1	20	theme	disc	119:122	arg1	back					156:159	back	156:159	back	156:159	Intervertebral disc degeneration and associated low back and neck pain is a ubiquitous health condition that affects millions of people world-wide, and causes high incidence of disability and enormous medical/societal costs.
29605718	1	20	theme	disc	119:122	arg1	condition					198:206	a ubiquitous health condition	178:206	a ubiquitous health condition that affects millions of people world-wide, and causes high incidence of disability and enormous medical/societal costs	178:326	Intervertebral disc degeneration and associated low back and neck pain is a ubiquitous health condition that affects millions of people world-wide, and causes high incidence of disability and enormous medical/societal costs.
29605718	4	21	with	8 weeks	839:845	arg1	severity					862:869	increased severity	852:869	increased severity	852:869	In SM/J mice, degenerative process was marked by decreased nucleus pulposus (NP) cellularity and changes in matrix composition at P7, 4, and 8 weeks with increased severity by 17 weeks.
29605718	8	22	theme	ARGxx	1272:1276	arg1	levels					1262:1267	The levels	1258:1267	The levels of ARGxx	1258:1276	The levels of ARGxx were increased in, indicating higher aggrecan turnover.
29605718	9	23	theme	chondrocyte	1502:1512	arg1	hypertrophy					1514:1524	chondrocyte hypertrophy	1502:1524	chondrocyte hypertrophy	1502:1524	Furthermore, an aberrant expression of collagen X and MMP13 was observed in the NP of SM/J mice, along with elevated expression of Col10a1, Ctgf, and Runx2, markers of chondrocyte hypertrophy.
29605718	5	24	theme	SM/J	984:987	arg1	mice					989:992	SM/J mice	984:992	SM/J mice	984:992	Distinctions between NP and annulus fibrosus (AF) or endplate cartilage were lost, and NP and AF of SM/J mice showed higher histological grades.
29605718	4	25	from	cellularity	779:789	arg1	composition					813:823	matrix composition	806:823	matrix composition	806:823	In SM/J mice, degenerative process was marked by decreased nucleus pulposus (NP) cellularity and changes in matrix composition at P7, 4, and 8 weeks with increased severity by 17 weeks.
29605718	2	26	theme	degenerative	507:518	arg1	process					520:526	the degenerative process	503:526	the degenerative process	503:526	However, lack of appropriate small animal models with spontaneous disease onset has impeded our ability to understand the pathogenetic mechanisms that characterize and drive the degenerative process.
29605718	7	27	with	replacement	1177:1187	arg1	tissue					1250:1255	collagenous fibrous tissue	1230:1255	collagenous fibrous tissue	1230:1255	Polarized microscopy and FTIR spectroscopy demonstrated replacement of glycosaminoglycan-rich NP matrix with collagenous fibrous tissue.
29605718	13	28	theme	SM/J	1987:1990	arg1	strain					1998:2003	SM/J mouse strain	1987:2003	SM/J mouse strain	1987:2003	Taken together, our results clearly demonstrate that SM/J mouse strain recapitulates many salient features of human disc degeneration, and serves as a novel small animal model.
29605718	13	28	theme	SM/J	1987:1990	arg1	model					2104:2108	a novel small animal model	2083:2108	a novel small animal model	2083:2108	Taken together, our results clearly demonstrate that SM/J mouse strain recapitulates many salient features of human disc degeneration, and serves as a novel small animal model.
29605718	9	29	theme	Col10a1	1465:1471	arg1	markers					1491:1497	Col10a1, Ctgf, and Runx2, markers	1465:1497	Col10a1, Ctgf, and Runx2, markers of chondrocyte hypertrophy	1465:1524	Furthermore, an aberrant expression of collagen X and MMP13 was observed in the NP of SM/J mice, along with elevated expression of Col10a1, Ctgf, and Runx2, markers of chondrocyte hypertrophy.
29605718	13	30	theme	disc	2050:2053	arg1	degeneration					2055:2066	human disc degeneration	2044:2066	human disc degeneration	2044:2066	Taken together, our results clearly demonstrate that SM/J mouse strain recapitulates many salient features of human disc degeneration, and serves as a novel small animal model.
29605718	0	31	theme	cell	70:73	arg1	fate					75:78	altered cell fate	62:78	altered cell fate	62:78	A novel mouse model of intervertebral disc degeneration shows altered cell fate and matrix homeostasis.
29605718	9	32	theme	Ctgf	1474:1477	arg1	markers					1491:1497	Col10a1, Ctgf, and Runx2, markers	1465:1497	Col10a1, Ctgf, and Runx2, markers of chondrocyte hypertrophy	1465:1524	Furthermore, an aberrant expression of collagen X and MMP13 was observed in the NP of SM/J mice, along with elevated expression of Col10a1, Ctgf, and Runx2, markers of chondrocyte hypertrophy.
29605718	4	33	theme	decreased	747:755	arg1	cellularity					779:789	decreased nucleus pulposus (NP) cellularity	747:789	decreased nucleus pulposus (NP) cellularity	747:789	In SM/J mice, degenerative process was marked by decreased nucleus pulposus (NP) cellularity and changes in matrix composition at P7, 4, and 8 weeks with increased severity by 17 weeks.
29605718	7	34	theme	FTIR	1146:1149	arg1	spectroscopy					1151:1162	FTIR spectroscopy	1146:1162	FTIR spectroscopy	1146:1162	Polarized microscopy and FTIR spectroscopy demonstrated replacement of glycosaminoglycan-rich NP matrix with collagenous fibrous tissue.
29605718	4	35	theme	pulposus	765:772	arg1	cellularity					779:789	decreased nucleus pulposus (NP) cellularity	747:789	decreased nucleus pulposus (NP) cellularity	747:789	In SM/J mice, degenerative process was marked by decreased nucleus pulposus (NP) cellularity and changes in matrix composition at P7, 4, and 8 weeks with increased severity by 17 weeks.
29605718	2	36	theme	disease	395:401	arg1	onset					403:407	spontaneous disease onset	383:407	spontaneous disease onset	383:407	However, lack of appropriate small animal models with spontaneous disease onset has impeded our ability to understand the pathogenetic mechanisms that characterize and drive the degenerative process.
29605718	10	37	theme	Alpl	1568:1571	arg1	expression					1537:1546	expression	1537:1546	expression of Enpp1 as well as Alpl	1537:1571	Likewise, expression of Enpp1 as well as Alpl was higher, suggesting NP cells of SM/J mice promote dystrophic mineralization.
29605718	1	38	theme	high	263:266	arg1	incidence					268:276	high incidence	263:276	high incidence of disability and enormous medical/societal costs	263:326	Intervertebral disc degeneration and associated low back and neck pain is a ubiquitous health condition that affects millions of people world-wide, and causes high incidence of disability and enormous medical/societal costs.
29605718	0	39	theme	mouse	8:12	arg1	model					14:18	A novel mouse model	0:18	A novel mouse model of intervertebral disc degeneration	0:54	A novel mouse model of intervertebral disc degeneration shows altered cell fate and matrix homeostasis.
29605718	9	40	theme	hypertrophy	1514:1524	arg1	markers					1491:1497	Col10a1, Ctgf, and Runx2, markers	1465:1497	Col10a1, Ctgf, and Runx2, markers of chondrocyte hypertrophy	1465:1524	Furthermore, an aberrant expression of collagen X and MMP13 was observed in the NP of SM/J mice, along with elevated expression of Col10a1, Ctgf, and Runx2, markers of chondrocyte hypertrophy.
29605718	12	41	theme	SM/J	1790:1793	arg1	discs					1795:1799	SM/J discs	1790:1799	SM/J discs	1790:1799	Importantly, SM/J discs were stiffer, had decreased height, and poor vertebral bone quality, suggesting compromised motion segment mechanical functionality.
29605718	2	42	with	lack	338:341	arg1	onset					403:407	spontaneous disease onset	383:407	spontaneous disease onset	383:407	However, lack of appropriate small animal models with spontaneous disease onset has impeded our ability to understand the pathogenetic mechanisms that characterize and drive the degenerative process.
29605718	3	43	theme	poor	631:634	arg1	capacities					649:658	their poor regenerative capacities	625:658	their poor regenerative capacities	625:658	We report, for the first time, early onset spontaneous disc degeneration in SM/J mice known for their poor regenerative capacities compared to "super-healer" LG/J mice.
29605718	4	44	theme	matrix	806:811	arg1	composition					813:823	matrix composition	806:823	matrix composition	806:823	In SM/J mice, degenerative process was marked by decreased nucleus pulposus (NP) cellularity and changes in matrix composition at P7, 4, and 8 weeks with increased severity by 17 weeks.
29605718	0	45	theme	disc	38:41	arg1	degeneration					43:54	intervertebral disc degeneration	23:54	intervertebral disc degeneration	23:54	A novel mouse model of intervertebral disc degeneration shows altered cell fate and matrix homeostasis.
29605718	1	46	theme	medical/societal	305:320	arg1	costs					322:326	enormous medical/societal costs	296:326	enormous medical/societal costs	296:326	Intervertebral disc degeneration and associated low back and neck pain is a ubiquitous health condition that affects millions of people world-wide, and causes high incidence of disability and enormous medical/societal costs.
29605718	3	47	theme	super-healer	673:684	arg1	mice					692:695	"super-healer" LG/J mice	672:695	"super-healer" LG/J mice	672:695	We report, for the first time, early onset spontaneous disc degeneration in SM/J mice known for their poor regenerative capacities compared to "super-healer" LG/J mice.
29605718	4	48	theme	NP	775:776	arg1	cellularity					779:789	decreased nucleus pulposus (NP) cellularity	747:789	decreased nucleus pulposus (NP) cellularity	747:789	In SM/J mice, degenerative process was marked by decreased nucleus pulposus (NP) cellularity and changes in matrix composition at P7, 4, and 8 weeks with increased severity by 17 weeks.
29605718	10	49	theme	SM/J	1608:1611	arg1	mice					1613:1616	SM/J mice	1608:1616	SM/J mice	1608:1616	Likewise, expression of Enpp1 as well as Alpl was higher, suggesting NP cells of SM/J mice promote dystrophic mineralization.
29605718	9	50	theme	aberrant	1350:1357	arg1	expression					1359:1368	an aberrant expression	1347:1368	an aberrant expression of collagen X and MMP13	1347:1392	Furthermore, an aberrant expression of collagen X and MMP13 was observed in the NP of SM/J mice, along with elevated expression of Col10a1, Ctgf, and Runx2, markers of chondrocyte hypertrophy.
29605718	7	51	theme	fibrous	1242:1248	arg1	tissue					1250:1255	collagenous fibrous tissue	1230:1255	collagenous fibrous tissue	1230:1255	Polarized microscopy and FTIR spectroscopy demonstrated replacement of glycosaminoglycan-rich NP matrix with collagenous fibrous tissue.
29605718	2	52	theme	animal	364:369	arg1	models					371:376	appropriate small animal models	346:376	appropriate small animal models	346:376	However, lack of appropriate small animal models with spontaneous disease onset has impeded our ability to understand the pathogenetic mechanisms that characterize and drive the degenerative process.
29605718	13	53	theme	animal	2097:2102	arg1	strain					1998:2003	SM/J mouse strain	1987:2003	SM/J mouse strain	1987:2003	Taken together, our results clearly demonstrate that SM/J mouse strain recapitulates many salient features of human disc degeneration, and serves as a novel small animal model.
29605718	13	53	theme	animal	2097:2102	arg1	model					2104:2108	a novel small animal model	2083:2108	a novel small animal model	2083:2108	Taken together, our results clearly demonstrate that SM/J mouse strain recapitulates many salient features of human disc degeneration, and serves as a novel small animal model.
29605718	2	54	theme	appropriate	346:356	arg1	models					371:376	appropriate small animal models	346:376	appropriate small animal models	346:376	However, lack of appropriate small animal models with spontaneous disease onset has impeded our ability to understand the pathogenetic mechanisms that characterize and drive the degenerative process.
29605718	1	55	theme	world-wide	240:249	arg1	millions					221:228	millions	221:228	millions of people world-wide	221:249	Intervertebral disc degeneration and associated low back and neck pain is a ubiquitous health condition that affects millions of people world-wide, and causes high incidence of disability and enormous medical/societal costs.
29605718	9	56	located	observed	1398:1405	arg1	NP					1414:1415	the NP	1410:1415	the NP of SM/J mice	1410:1428	Furthermore, an aberrant expression of collagen X and MMP13 was observed in the NP of SM/J mice, along with elevated expression of Col10a1, Ctgf, and Runx2, markers of chondrocyte hypertrophy.
29605718	9	56	located	observed	1398:1405	arg2	expression					1359:1368	an aberrant expression	1347:1368	an aberrant expression of collagen X and MMP13	1347:1392	Furthermore, an aberrant expression of collagen X and MMP13 was observed in the NP of SM/J mice, along with elevated expression of Col10a1, Ctgf, and Runx2, markers of chondrocyte hypertrophy.
29605718	7	57	theme	NP	1215:1216	arg1	matrix					1218:1223	glycosaminoglycan-rich NP matrix	1192:1223	glycosaminoglycan-rich NP matrix	1192:1223	Polarized microscopy and FTIR spectroscopy demonstrated replacement of glycosaminoglycan-rich NP matrix with collagenous fibrous tissue.
29605718	3	58	theme	LG/J	687:690	arg1	mice					692:695	"super-healer" LG/J mice	672:695	"super-healer" LG/J mice	672:695	We report, for the first time, early onset spontaneous disc degeneration in SM/J mice known for their poor regenerative capacities compared to "super-healer" LG/J mice.
29605718	9	59	theme	MMP13	1388:1392	arg1	expression					1359:1368	an aberrant expression	1347:1368	an aberrant expression of collagen X and MMP13	1347:1392	Furthermore, an aberrant expression of collagen X and MMP13 was observed in the NP of SM/J mice, along with elevated expression of Col10a1, Ctgf, and Runx2, markers of chondrocyte hypertrophy.
29605718	12	60	theme	segment	1900:1906	arg1	functionality					1919:1931	compromised motion segment mechanical functionality	1881:1931	compromised motion segment mechanical functionality	1881:1931	Importantly, SM/J discs were stiffer, had decreased height, and poor vertebral bone quality, suggesting compromised motion segment mechanical functionality.
29605718	13	61	theme	degeneration	2055:2066	arg1	features					2032:2039	many salient features	2019:2039	many salient features of human disc degeneration	2019:2066	Taken together, our results clearly demonstrate that SM/J mouse strain recapitulates many salient features of human disc degeneration, and serves as a novel small animal model.
29605718	1	62	theme	neck	165:168	arg1	pain					170:173	neck pain	165:173	neck pain	165:173	Intervertebral disc degeneration and associated low back and neck pain is a ubiquitous health condition that affects millions of people world-wide, and causes high incidence of disability and enormous medical/societal costs.
29605718	6	63	theme	phenotypic	1091:1100	arg1	expression					1109:1118	decreased phenotypic marker expression	1081:1118	decreased phenotypic marker expression	1081:1118	There was increased NP cell death in SM/J mice with decreased phenotypic marker expression.
29605718	3	64	theme	early	560:564	arg1	degeneration					589:600	early onset spontaneous disc degeneration	560:600	early onset spontaneous disc degeneration in SM/J mice known for their poor regenerative capacities compared to "super-healer" LG/J mice	560:695	We report, for the first time, early onset spontaneous disc degeneration in SM/J mice known for their poor regenerative capacities compared to "super-healer" LG/J mice.
29605718	12	65	dep	stiffer	1806:1812	arg1	decreased					1819:1827	decreased	1819:1827	decreased	1819:1827	Importantly, SM/J discs were stiffer, had decreased height, and poor vertebral bone quality, suggesting compromised motion segment mechanical functionality.
29605718	1	66	theme	ubiquitous	180:189	arg1	degeneration					124:135	Intervertebral disc degeneration	104:135	Intervertebral disc degeneration	104:135	Intervertebral disc degeneration and associated low back and neck pain is a ubiquitous health condition that affects millions of people world-wide, and causes high incidence of disability and enormous medical/societal costs.
29605718	1	66	theme	ubiquitous	180:189	arg1	back					156:159	back	156:159	back	156:159	Intervertebral disc degeneration and associated low back and neck pain is a ubiquitous health condition that affects millions of people world-wide, and causes high incidence of disability and enormous medical/societal costs.
29605718	1	66	theme	ubiquitous	180:189	arg1	condition					198:206	a ubiquitous health condition	178:206	a ubiquitous health condition that affects millions of people world-wide, and causes high incidence of disability and enormous medical/societal costs	178:326	Intervertebral disc degeneration and associated low back and neck pain is a ubiquitous health condition that affects millions of people world-wide, and causes high incidence of disability and enormous medical/societal costs.
29605718	12	67	theme	compromised	1881:1891	arg1	functionality					1919:1931	compromised motion segment mechanical functionality	1881:1931	compromised motion segment mechanical functionality	1881:1931	Importantly, SM/J discs were stiffer, had decreased height, and poor vertebral bone quality, suggesting compromised motion segment mechanical functionality.
29605718	6	68	theme	SM/J	1066:1069	arg1	mice					1071:1074	SM/J mice	1066:1074	SM/J mice with decreased phenotypic marker expression	1066:1118	There was increased NP cell death in SM/J mice with decreased phenotypic marker expression.
29605718	11	69	theme	NP	1713:1714	arg1	survival					1721:1728	NP cell survival	1713:1728	NP cell survival	1713:1728	There was also a decrease in several pathways necessary for NP cell survival and function including Wnt and VEGF signaling.
29605718	5	70	theme	higher	1001:1006	arg1	grades					1021:1026	higher histological grades	1001:1026	higher histological grades	1001:1026	Distinctions between NP and annulus fibrosus (AF) or endplate cartilage were lost, and NP and AF of SM/J mice showed higher histological grades.
29605718	9	71	theme	mice	1425:1428	arg1	NP					1414:1415	the NP	1410:1415	the NP of SM/J mice	1410:1428	Furthermore, an aberrant expression of collagen X and MMP13 was observed in the NP of SM/J mice, along with elevated expression of Col10a1, Ctgf, and Runx2, markers of chondrocyte hypertrophy.
29605718	12	72	theme	poor	1841:1844	arg1	quality					1861:1867	poor vertebral bone quality	1841:1867	poor vertebral bone quality	1841:1867	Importantly, SM/J discs were stiffer, had decreased height, and poor vertebral bone quality, suggesting compromised motion segment mechanical functionality.
29605718	6	73	with	mice	1071:1074	arg1	expression					1109:1118	decreased phenotypic marker expression	1081:1118	decreased phenotypic marker expression	1081:1118	There was increased NP cell death in SM/J mice with decreased phenotypic marker expression.
29605718	10	74	theme	Enpp1	1551:1555	arg1	expression					1537:1546	expression	1537:1546	expression of Enpp1 as well as Alpl	1537:1571	Likewise, expression of Enpp1 as well as Alpl was higher, suggesting NP cells of SM/J mice promote dystrophic mineralization.
29605718	11	75	theme	Wnt	1753:1755	arg1	signaling					1766:1774	Wnt and VEGF signaling	1753:1774	signaling	1766:1774	There was also a decrease in several pathways necessary for NP cell survival and function including Wnt and VEGF signaling.
29605718	3	76	theme	spontaneous	572:582	arg1	degeneration					589:600	early onset spontaneous disc degeneration	560:600	early onset spontaneous disc degeneration in SM/J mice known for their poor regenerative capacities compared to "super-healer" LG/J mice	560:695	We report, for the first time, early onset spontaneous disc degeneration in SM/J mice known for their poor regenerative capacities compared to "super-healer" LG/J mice.
29605718	1	77	theme	Intervertebral	104:117	arg1	degeneration					124:135	Intervertebral disc degeneration	104:135	Intervertebral disc degeneration	104:135	Intervertebral disc degeneration and associated low back and neck pain is a ubiquitous health condition that affects millions of people world-wide, and causes high incidence of disability and enormous medical/societal costs.
29605718	1	77	theme	Intervertebral	104:117	arg1	back					156:159	back	156:159	back	156:159	Intervertebral disc degeneration and associated low back and neck pain is a ubiquitous health condition that affects millions of people world-wide, and causes high incidence of disability and enormous medical/societal costs.
29605718	1	77	theme	Intervertebral	104:117	arg1	condition					198:206	a ubiquitous health condition	178:206	a ubiquitous health condition that affects millions of people world-wide, and causes high incidence of disability and enormous medical/societal costs	178:326	Intervertebral disc degeneration and associated low back and neck pain is a ubiquitous health condition that affects millions of people world-wide, and causes high incidence of disability and enormous medical/societal costs.
29605718	12	78	theme	bone	1856:1859	arg1	quality					1861:1867	poor vertebral bone quality	1841:1867	poor vertebral bone quality	1841:1867	Importantly, SM/J discs were stiffer, had decreased height, and poor vertebral bone quality, suggesting compromised motion segment mechanical functionality.
29605718	13	79	theme	salient	2024:2030	arg1	features					2032:2039	many salient features	2019:2039	many salient features of human disc degeneration	2019:2066	Taken together, our results clearly demonstrate that SM/J mouse strain recapitulates many salient features of human disc degeneration, and serves as a novel small animal model.
29605718	9	80	theme	elevated	1442:1449	arg1	expression					1451:1460	elevated expression	1442:1460	elevated expression of Col10a1, Ctgf, and Runx2, markers of chondrocyte hypertrophy	1442:1524	Furthermore, an aberrant expression of collagen X and MMP13 was observed in the NP of SM/J mice, along with elevated expression of Col10a1, Ctgf, and Runx2, markers of chondrocyte hypertrophy.
29605718	11	81	from	decrease	1670:1677	arg1	pathways					1690:1697	several pathways	1682:1697	several pathways necessary for NP cell survival and function including Wnt and VEGF signaling	1682:1774	There was also a decrease in several pathways necessary for NP cell survival and function including Wnt and VEGF signaling.
29605718	8	82	theme	higher	1308:1313	arg1	turnover					1324:1331	higher aggrecan turnover	1308:1331	higher aggrecan turnover	1308:1331	The levels of ARGxx were increased in, indicating higher aggrecan turnover.
29605718	7	83	theme	collagenous	1230:1240	arg1	tissue					1250:1255	collagenous fibrous tissue	1230:1255	collagenous fibrous tissue	1230:1255	Polarized microscopy and FTIR spectroscopy demonstrated replacement of glycosaminoglycan-rich NP matrix with collagenous fibrous tissue.
29605718	0	84	theme	altered	62:68	arg1	fate					75:78	altered cell fate	62:78	altered cell fate	62:78	A novel mouse model of intervertebral disc degeneration shows altered cell fate and matrix homeostasis.
29605718	5	85	theme	mice	989:992	arg1	AF					978:979	AF	978:979	AF	978:979	Distinctions between NP and annulus fibrosus (AF) or endplate cartilage were lost, and NP and AF of SM/J mice showed higher histological grades.
29605718	5	85	theme	mice	989:992	arg1	NP					971:972	NP	971:972	NP	971:972	Distinctions between NP and annulus fibrosus (AF) or endplate cartilage were lost, and NP and AF of SM/J mice showed higher histological grades.
29605718	3	86	theme	SM/J	605:608	arg1	mice					610:613	SM/J mice	605:613	SM/J mice known for their poor regenerative capacities	605:658	We report, for the first time, early onset spontaneous disc degeneration in SM/J mice known for their poor regenerative capacities compared to "super-healer" LG/J mice.
29605718	13	87	theme	novel	2085:2089	arg1	strain					1998:2003	SM/J mouse strain	1987:2003	SM/J mouse strain	1987:2003	Taken together, our results clearly demonstrate that SM/J mouse strain recapitulates many salient features of human disc degeneration, and serves as a novel small animal model.
29605718	13	87	theme	novel	2085:2089	arg1	model					2104:2108	a novel small animal model	2083:2108	a novel small animal model	2083:2108	Taken together, our results clearly demonstrate that SM/J mouse strain recapitulates many salient features of human disc degeneration, and serves as a novel small animal model.
29605718	13	88	theme	mouse	1992:1996	arg1	strain					1998:2003	SM/J mouse strain	1987:2003	SM/J mouse strain	1987:2003	Taken together, our results clearly demonstrate that SM/J mouse strain recapitulates many salient features of human disc degeneration, and serves as a novel small animal model.
29605718	13	88	theme	mouse	1992:1996	arg1	model					2104:2108	a novel small animal model	2083:2108	a novel small animal model	2083:2108	Taken together, our results clearly demonstrate that SM/J mouse strain recapitulates many salient features of human disc degeneration, and serves as a novel small animal model.
29605718	4	89	with	P7	828:829	arg1	severity					862:869	increased severity	852:869	increased severity	852:869	In SM/J mice, degenerative process was marked by decreased nucleus pulposus (NP) cellularity and changes in matrix composition at P7, 4, and 8 weeks with increased severity by 17 weeks.
29605718	11	90	theme	necessary	1699:1707	arg1	pathways					1690:1697	several pathways	1682:1697	several pathways necessary for NP cell survival and function including Wnt and VEGF signaling	1682:1774	There was also a decrease in several pathways necessary for NP cell survival and function including Wnt and VEGF signaling.
29605718	0	91	theme	matrix	84:89	arg1	homeostasis					91:101	matrix homeostasis	84:101	matrix homeostasis	84:101	A novel mouse model of intervertebral disc degeneration shows altered cell fate and matrix homeostasis.
29605718	6	92	theme	NP	1049:1050	arg1	death					1057:1061	NP cell death	1049:1061	NP cell death	1049:1061	There was increased NP cell death in SM/J mice with decreased phenotypic marker expression.
29605718	7	93	theme	Polarized	1121:1129	arg1	microscopy					1131:1140	Polarized microscopy	1121:1140	Polarized microscopy	1121:1140	Polarized microscopy and FTIR spectroscopy demonstrated replacement of glycosaminoglycan-rich NP matrix with collagenous fibrous tissue.
29605718	1	94	theme	health	191:196	arg1	degeneration					124:135	Intervertebral disc degeneration	104:135	Intervertebral disc degeneration	104:135	Intervertebral disc degeneration and associated low back and neck pain is a ubiquitous health condition that affects millions of people world-wide, and causes high incidence of disability and enormous medical/societal costs.
29605718	1	94	theme	health	191:196	arg1	back					156:159	back	156:159	back	156:159	Intervertebral disc degeneration and associated low back and neck pain is a ubiquitous health condition that affects millions of people world-wide, and causes high incidence of disability and enormous medical/societal costs.
29605718	1	94	theme	health	191:196	arg1	condition					198:206	a ubiquitous health condition	178:206	a ubiquitous health condition that affects millions of people world-wide, and causes high incidence of disability and enormous medical/societal costs	178:326	Intervertebral disc degeneration and associated low back and neck pain is a ubiquitous health condition that affects millions of people world-wide, and causes high incidence of disability and enormous medical/societal costs.
29605718	9	95	theme	Runx2	1484:1488	arg1	markers					1491:1497	Col10a1, Ctgf, and Runx2, markers	1465:1497	Col10a1, Ctgf, and Runx2, markers of chondrocyte hypertrophy	1465:1524	Furthermore, an aberrant expression of collagen X and MMP13 was observed in the NP of SM/J mice, along with elevated expression of Col10a1, Ctgf, and Runx2, markers of chondrocyte hypertrophy.
29605718	11	96	theme	several	1682:1688	arg1	pathways					1690:1697	several pathways	1682:1697	several pathways necessary for NP cell survival and function including Wnt and VEGF signaling	1682:1774	There was also a decrease in several pathways necessary for NP cell survival and function including Wnt and VEGF signaling.
29605718	8	97	theme	aggrecan	1315:1322	arg1	turnover					1324:1331	higher aggrecan turnover	1308:1331	higher aggrecan turnover	1308:1331	The levels of ARGxx were increased in, indicating higher aggrecan turnover.
29605718	2	98	theme	spontaneous	383:393	arg1	onset					403:407	spontaneous disease onset	383:407	spontaneous disease onset	383:407	However, lack of appropriate small animal models with spontaneous disease onset has impeded our ability to understand the pathogenetic mechanisms that characterize and drive the degenerative process.
29605718	0	99	theme	novel	2:6	arg1	model					14:18	A novel mouse model	0:18	A novel mouse model of intervertebral disc degeneration	0:54	A novel mouse model of intervertebral disc degeneration shows altered cell fate and matrix homeostasis.
29605718	9	100	theme	markers	1491:1497	arg1	expression					1451:1460	elevated expression	1442:1460	elevated expression of Col10a1, Ctgf, and Runx2, markers of chondrocyte hypertrophy	1442:1524	Furthermore, an aberrant expression of collagen X and MMP13 was observed in the NP of SM/J mice, along with elevated expression of Col10a1, Ctgf, and Runx2, markers of chondrocyte hypertrophy.
29605718	4	101	theme	degenerative	712:723	arg1	process					725:731	degenerative process	712:731	degenerative process	712:731	In SM/J mice, degenerative process was marked by decreased nucleus pulposus (NP) cellularity and changes in matrix composition at P7, 4, and 8 weeks with increased severity by 17 weeks.
29605718	2	102	theme	models	371:376	arg1	lack					338:341	lack	338:341	lack of appropriate small animal models with spontaneous disease onset	338:407	However, lack of appropriate small animal models with spontaneous disease onset has impeded our ability to understand the pathogenetic mechanisms that characterize and drive the degenerative process.
29605718	5	103	theme	annulus	912:918	arg1	fibrosus					920:927	annulus fibrosus	912:927	annulus fibrosus	912:927	Distinctions between NP and annulus fibrosus (AF) or endplate cartilage were lost, and NP and AF of SM/J mice showed higher histological grades.
29605718	3	104	theme	regenerative	636:647	arg1	capacities					649:658	their poor regenerative capacities	625:658	their poor regenerative capacities	625:658	We report, for the first time, early onset spontaneous disc degeneration in SM/J mice known for their poor regenerative capacities compared to "super-healer" LG/J mice.
29605718	3	105	from	degeneration	589:600	arg1	mice					610:613	SM/J mice	605:613	SM/J mice known for their poor regenerative capacities	605:658	We report, for the first time, early onset spontaneous disc degeneration in SM/J mice known for their poor regenerative capacities compared to "super-healer" LG/J mice.
29605718	10	106	theme	NP	1596:1597	arg1	cells					1599:1603	NP cells	1596:1603	NP cells of SM/J mice	1596:1616	Likewise, expression of Enpp1 as well as Alpl was higher, suggesting NP cells of SM/J mice promote dystrophic mineralization.
29605718	0	107	theme	intervertebral	23:36	arg1	degeneration					43:54	intervertebral disc degeneration	23:54	intervertebral disc degeneration	23:54	A novel mouse model of intervertebral disc degeneration shows altered cell fate and matrix homeostasis.
29605718	1	108	theme	disability	281:290	arg1	incidence					268:276	high incidence	263:276	high incidence of disability and enormous medical/societal costs	263:326	Intervertebral disc degeneration and associated low back and neck pain is a ubiquitous health condition that affects millions of people world-wide, and causes high incidence of disability and enormous medical/societal costs.
29605718	0	109	theme	degeneration	43:54	arg1	model					14:18	A novel mouse model	0:18	A novel mouse model of intervertebral disc degeneration	0:54	A novel mouse model of intervertebral disc degeneration shows altered cell fate and matrix homeostasis.
29605718	1	110	theme	enormous	296:303	arg1	costs					322:326	enormous medical/societal costs	296:326	enormous medical/societal costs	296:326	Intervertebral disc degeneration and associated low back and neck pain is a ubiquitous health condition that affects millions of people world-wide, and causes high incidence of disability and enormous medical/societal costs.
29605718	2	111	theme	pathogenetic	451:462	arg1	mechanisms					464:473	the pathogenetic mechanisms	447:473	the pathogenetic mechanisms that characterize and drive the degenerative process	447:526	However, lack of appropriate small animal models with spontaneous disease onset has impeded our ability to understand the pathogenetic mechanisms that characterize and drive the degenerative process.
29605718	10	112	theme	mice	1613:1616	arg1	cells					1599:1603	NP cells	1596:1603	NP cells of SM/J mice	1596:1616	Likewise, expression of Enpp1 as well as Alpl was higher, suggesting NP cells of SM/J mice promote dystrophic mineralization.
29605718	1	113	theme	costs	322:326	arg1	incidence					268:276	high incidence	263:276	high incidence of disability and enormous medical/societal costs	263:326	Intervertebral disc degeneration and associated low back and neck pain is a ubiquitous health condition that affects millions of people world-wide, and causes high incidence of disability and enormous medical/societal costs.
29605718	5	114	theme	endplate	937:944	arg1	cartilage					946:954	endplate cartilage	937:954	endplate cartilage	937:954	Distinctions between NP and annulus fibrosus (AF) or endplate cartilage were lost, and NP and AF of SM/J mice showed higher histological grades.
29605718	6	115	theme	cell	1052:1055	arg1	death					1057:1061	NP cell death	1049:1061	NP cell death	1049:1061	There was increased NP cell death in SM/J mice with decreased phenotypic marker expression.
29605718	4	116	theme	nucleus	757:763	arg1	cellularity					779:789	decreased nucleus pulposus (NP) cellularity	747:789	decreased nucleus pulposus (NP) cellularity	747:789	In SM/J mice, degenerative process was marked by decreased nucleus pulposus (NP) cellularity and changes in matrix composition at P7, 4, and 8 weeks with increased severity by 17 weeks.
29605718	11	117	theme	VEGF	1761:1764	arg1	signaling					1766:1774	Wnt and VEGF signaling	1753:1774	signaling	1766:1774	There was also a decrease in several pathways necessary for NP cell survival and function including Wnt and VEGF signaling.
29605718	13	118	theme	small	2091:2095	arg1	strain					1998:2003	SM/J mouse strain	1987:2003	SM/J mouse strain	1987:2003	Taken together, our results clearly demonstrate that SM/J mouse strain recapitulates many salient features of human disc degeneration, and serves as a novel small animal model.
29605718	13	118	theme	small	2091:2095	arg1	model					2104:2108	a novel small animal model	2083:2108	a novel small animal model	2083:2108	Taken together, our results clearly demonstrate that SM/J mouse strain recapitulates many salient features of human disc degeneration, and serves as a novel small animal model.
29605718	7	119	theme	matrix	1218:1223	arg1	replacement					1177:1187	replacement	1177:1187	replacement of glycosaminoglycan-rich NP matrix with collagenous fibrous tissue	1177:1255	Polarized microscopy and FTIR spectroscopy demonstrated replacement of glycosaminoglycan-rich NP matrix with collagenous fibrous tissue.
29605718	1	120	theme	people	233:238	arg1	world-wide					240:249	people world-wide	233:249	people world-wide	233:249	Intervertebral disc degeneration and associated low back and neck pain is a ubiquitous health condition that affects millions of people world-wide, and causes high incidence of disability and enormous medical/societal costs.
29605718	2	121	theme	small	358:362	arg1	models					371:376	appropriate small animal models	346:376	appropriate small animal models	346:376	However, lack of appropriate small animal models with spontaneous disease onset has impeded our ability to understand the pathogenetic mechanisms that characterize and drive the degenerative process.
29605718	9	122	theme	collagen	1373:1380	arg1	X					1382:1382	collagen X	1373:1382	collagen X	1373:1382	Furthermore, an aberrant expression of collagen X and MMP13 was observed in the NP of SM/J mice, along with elevated expression of Col10a1, Ctgf, and Runx2, markers of chondrocyte hypertrophy.
31211931	0	0	theme	mouse	82:86	arg1	epithelium					94:103	injured mouse colon epithelium	74:103	injured mouse colon epithelium	74:103	In vivo evaluation of scaffolds compatible for colonoid engraftments onto injured mouse colon epithelium.
31211931	9	1	theme	safety	1604:1609	arg1	evidence					1588:1595	evidence	1588:1595	evidence of the safety and utility of the clinical application of colonoid-based therapeutics	1588:1680	This study suggests new methods for and provides evidence of the safety and utility of the clinical application of colonoid-based therapeutics.
31211931	2	2	used	used	362:365	arg2	Matrigel					283:290	Matrigel	283:290	Matrigel	283:290	Matrigel, which is used as a transplantation scaffold for colonoids, cannot be used in clinical applications because of its undefined composition and tumorigenicity.
31211931	2	2	used	used	362:365	arg2	scaffold					328:335	a transplantation scaffold	310:335	a transplantation scaffold for colonoids	310:349	Matrigel, which is used as a transplantation scaffold for colonoids, cannot be used in clinical applications because of its undefined composition and tumorigenicity.
31211931	5	3	theme	colon	907:911	arg1	mucosa					913:918	injured colon mucosa	899:918	injured colon mucosa	899:918	To identify scaffolds suitable for colonoid engraftment in injured colon mucosa, the success rates of transplantation and secondary EGFP colonoid formation were measured, and the scaffolds' mediated toxicity in vitro and in vivo was observed in recipient mice.
31211931	4	4	theme	colon	584:588	arg1	crypt					590:594	The colon crypt	580:594	The colon crypt	580:594	The colon crypt was isolated and cultured from C57BL/6-Tg[CAG enhanced green fluorescent protein (EGFP)131Osb/LeySopJ mice into EGFP + colonoids and subsequently transplanted into the EDTA colitis mouse model using gelatin, collagen, or fibrin glue scaffolds.
31211931	0	5	theme	injured	74:80	arg1	epithelium					94:103	injured mouse colon epithelium	74:103	injured mouse colon epithelium	74:103	In vivo evaluation of scaffolds compatible for colonoid engraftments onto injured mouse colon epithelium.
31211931	11	6	dep	In	1971:1972	arg1	vivo					1974:1977	vivo	1974:1977	vivo	1974:1977	In vivo evaluation of scaffolds compatible for colonoid engraftments onto injured mouse colon epithelium.
31211931	2	7	theme	transplantation	312:326	arg1	scaffold					328:335	a transplantation scaffold	310:335	a transplantation scaffold for colonoids	310:349	Matrigel, which is used as a transplantation scaffold for colonoids, cannot be used in clinical applications because of its undefined composition and tumorigenicity.
31211931	2	7	theme	transplantation	312:326	arg1	Matrigel					283:290	Matrigel	283:290	Matrigel	283:290	Matrigel, which is used as a transplantation scaffold for colonoids, cannot be used in clinical applications because of its undefined composition and tumorigenicity.
31211931	3	8	from	transplantation	534:548	arg1	mucosa					572:577	damaged intestinal mucosa	553:577	damaged intestinal mucosa	553:577	This study identifies clinically available scaffolds that are effective for colonoid transplantation in damaged intestinal mucosa.
31211931	6	9	theme	colitis	1188:1194	arg1	model					1202:1206	the EDTA colitis mouse model	1179:1206	the EDTA colitis mouse model	1179:1206	When colonoids were transplanted with gelatin, collagen, and fibrin glue into the EDTA colitis mouse model, all groups were found to be successfully engrafted.
31211931	9	10	theme	utility	1615:1621	arg1	evidence					1588:1595	evidence	1588:1595	evidence of the safety and utility of the clinical application of colonoid-based therapeutics	1588:1680	This study suggests new methods for and provides evidence of the safety and utility of the clinical application of colonoid-based therapeutics.
31211931	4	11	theme	mouse	777:781	arg1	model					783:787	the EDTA colitis mouse model	760:787	the EDTA colitis mouse model using gelatin, collagen, or fibrin glue scaffolds	760:837	The colon crypt was isolated and cultured from C57BL/6-Tg[CAG enhanced green fluorescent protein (EGFP)131Osb/LeySopJ mice into EGFP + colonoids and subsequently transplanted into the EDTA colitis mouse model using gelatin, collagen, or fibrin glue scaffolds.
31211931	3	12	theme	intestinal	561:570	arg1	mucosa					572:577	damaged intestinal mucosa	553:577	damaged intestinal mucosa	553:577	This study identifies clinically available scaffolds that are effective for colonoid transplantation in damaged intestinal mucosa.
31211931	10	13	dep	digestive-system	1832:1847	arg1	Kim					1882:1884	Kim	1882:1884	Kim	1882:1884	Furthermore, the methods introduced in this study will be helpful in developing cell treatment using the esophagus or a stomach organoid for various digestive-system diseases.-Jee, J., Jeong, S. Y., Kim, H. K., Choi, S. Y., Jeong, S., Lee, J., Ko, J. S., Kim, M. S., Kwon, M.-S., Yoo, J.
31211931	10	13	dep	digestive-system	1832:1847	arg1	Lee					1918:1920	Lee	1918:1920	Lee	1918:1920	Furthermore, the methods introduced in this study will be helpful in developing cell treatment using the esophagus or a stomach organoid for various digestive-system diseases.-Jee, J., Jeong, S. Y., Kim, H. K., Choi, S. Y., Jeong, S., Lee, J., Ko, J. S., Kim, M. S., Kwon, M.-S., Yoo, J.
31211931	10	13	dep	digestive-system	1832:1847	arg1	J.					1931:1932	J.	1931:1932	J.	1931:1932	Furthermore, the methods introduced in this study will be helpful in developing cell treatment using the esophagus or a stomach organoid for various digestive-system diseases.-Jee, J., Jeong, S. Y., Kim, H. K., Choi, S. Y., Jeong, S., Lee, J., Ko, J. S., Kim, M. S., Kwon, M.-S., Yoo, J.
31211931	10	13	dep	digestive-system	1832:1847	arg1	S.					1900:1901	S.	1900:1901	S.	1900:1901	Furthermore, the methods introduced in this study will be helpful in developing cell treatment using the esophagus or a stomach organoid for various digestive-system diseases.-Jee, J., Jeong, S. Y., Kim, H. K., Choi, S. Y., Jeong, S., Lee, J., Ko, J. S., Kim, M. S., Kwon, M.-S., Yoo, J.
31211931	10	13	dep	digestive-system	1832:1847	arg1	H.					1887:1888	H.	1887:1888	H.	1887:1888	Furthermore, the methods introduced in this study will be helpful in developing cell treatment using the esophagus or a stomach organoid for various digestive-system diseases.-Jee, J., Jeong, S. Y., Kim, H. K., Choi, S. Y., Jeong, S., Lee, J., Ko, J. S., Kim, M. S., Kwon, M.-S., Yoo, J.
31211931	10	13	dep	digestive-system	1832:1847	arg1	S.					1934:1935	S.	1934:1935	S.	1934:1935	Furthermore, the methods introduced in this study will be helpful in developing cell treatment using the esophagus or a stomach organoid for various digestive-system diseases.-Jee, J., Jeong, S. Y., Kim, H. K., Choi, S. Y., Jeong, S., Lee, J., Ko, J. S., Kim, M. S., Kwon, M.-S., Yoo, J.
31211931	10	13	dep	digestive-system	1832:1847	arg1	Jeong					1868:1872	Jeong	1868:1872	Jeong	1868:1872	Furthermore, the methods introduced in this study will be helpful in developing cell treatment using the esophagus or a stomach organoid for various digestive-system diseases.-Jee, J., Jeong, S. Y., Kim, H. K., Choi, S. Y., Jeong, S., Lee, J., Ko, J. S., Kim, M. S., Kwon, M.-S., Yoo, J.
31211931	10	13	dep	digestive-system	1832:1847	arg1	Jeong					1907:1911	Jeong	1907:1911	Jeong	1907:1911	Furthermore, the methods introduced in this study will be helpful in developing cell treatment using the esophagus or a stomach organoid for various digestive-system diseases.-Jee, J., Jeong, S. Y., Kim, H. K., Choi, S. Y., Jeong, S., Lee, J., Ko, J. S., Kim, M. S., Kwon, M.-S., Yoo, J.
31211931	10	13	dep	digestive-system	1832:1847	arg1	S.					1875:1876	S.	1875:1876	S.	1875:1876	Furthermore, the methods introduced in this study will be helpful in developing cell treatment using the esophagus or a stomach organoid for various digestive-system diseases.-Jee, J., Jeong, S. Y., Kim, H. K., Choi, S. Y., Jeong, S., Lee, J., Ko, J. S., Kim, M. S., Kwon, M.-S., Yoo, J.
31211931	10	13	dep	digestive-system	1832:1847	arg1	J.					1923:1924	J.	1923:1924	J.	1923:1924	Furthermore, the methods introduced in this study will be helpful in developing cell treatment using the esophagus or a stomach organoid for various digestive-system diseases.-Jee, J., Jeong, S. Y., Kim, H. K., Choi, S. Y., Jeong, S., Lee, J., Ko, J. S., Kim, M. S., Kwon, M.-S., Yoo, J.
31211931	10	13	dep	digestive-system	1832:1847	arg1	Ko					1927:1928	Ko	1927:1928	Ko	1927:1928	Furthermore, the methods introduced in this study will be helpful in developing cell treatment using the esophagus or a stomach organoid for various digestive-system diseases.-Jee, J., Jeong, S. Y., Kim, H. K., Choi, S. Y., Jeong, S., Lee, J., Ko, J. S., Kim, M. S., Kwon, M.-S., Yoo, J.
31211931	10	13	dep	digestive-system	1832:1847	arg1	Choi					1894:1897	Choi	1894:1897	Choi	1894:1897	Furthermore, the methods introduced in this study will be helpful in developing cell treatment using the esophagus or a stomach organoid for various digestive-system diseases.-Jee, J., Jeong, S. Y., Kim, H. K., Choi, S. Y., Jeong, S., Lee, J., Ko, J. S., Kim, M. S., Kwon, M.-S., Yoo, J.
31211931	10	13	dep	digestive-system	1832:1847	arg1	S.					1914:1915	S.	1914:1915	S.	1914:1915	Furthermore, the methods introduced in this study will be helpful in developing cell treatment using the esophagus or a stomach organoid for various digestive-system diseases.-Jee, J., Jeong, S. Y., Kim, H. K., Choi, S. Y., Jeong, S., Lee, J., Ko, J. S., Kim, M. S., Kwon, M.-S., Yoo, J.
31211931	0	14	theme	colon	88:92	arg1	epithelium					94:103	injured mouse colon epithelium	74:103	injured mouse colon epithelium	74:103	In vivo evaluation of scaffolds compatible for colonoid engraftments onto injured mouse colon epithelium.
31211931	5	15	theme	recipient	1085:1093	arg1	mice					1095:1098	recipient mice	1085:1098	recipient mice	1085:1098	To identify scaffolds suitable for colonoid engraftment in injured colon mucosa, the success rates of transplantation and secondary EGFP colonoid formation were measured, and the scaffolds' mediated toxicity in vitro and in vivo was observed in recipient mice.
31211931	5	16	theme	colonoid	977:984	arg1	formation					986:994	secondary EGFP colonoid formation	962:994	secondary EGFP colonoid formation	962:994	To identify scaffolds suitable for colonoid engraftment in injured colon mucosa, the success rates of transplantation and secondary EGFP colonoid formation were measured, and the scaffolds' mediated toxicity in vitro and in vivo was observed in recipient mice.
31211931	4	17	theme	colitis	769:775	arg1	model					783:787	the EDTA colitis mouse model	760:787	the EDTA colitis mouse model using gelatin, collagen, or fibrin glue scaffolds	760:837	The colon crypt was isolated and cultured from C57BL/6-Tg[CAG enhanced green fluorescent protein (EGFP)131Osb/LeySopJ mice into EGFP + colonoids and subsequently transplanted into the EDTA colitis mouse model using gelatin, collagen, or fibrin glue scaffolds.
31211931	11	18	theme	mouse	2053:2057	arg1	epithelium					2065:2074	injured mouse colon epithelium	2045:2074	injured mouse colon epithelium	2045:2074	In vivo evaluation of scaffolds compatible for colonoid engraftments onto injured mouse colon epithelium.
31211931	5	19	theme	formation	986:994	arg1	rates					933:937	the success rates	921:937	the success rates of transplantation and secondary EGFP colonoid formation	921:994	To identify scaffolds suitable for colonoid engraftment in injured colon mucosa, the success rates of transplantation and secondary EGFP colonoid formation were measured, and the scaffolds' mediated toxicity in vitro and in vivo was observed in recipient mice.
31211931	5	20	theme	colonoid	875:882	arg1	engraftment					884:894	colonoid engraftment	875:894	colonoid engraftment in injured colon mucosa	875:918	To identify scaffolds suitable for colonoid engraftment in injured colon mucosa, the success rates of transplantation and secondary EGFP colonoid formation were measured, and the scaffolds' mediated toxicity in vitro and in vivo was observed in recipient mice.
31211931	5	21	theme	suitable	862:869	arg1	scaffolds					852:860	scaffolds	852:860	scaffolds suitable for colonoid engraftment in injured colon mucosa	852:918	To identify scaffolds suitable for colonoid engraftment in injured colon mucosa, the success rates of transplantation and secondary EGFP colonoid formation were measured, and the scaffolds' mediated toxicity in vitro and in vivo was observed in recipient mice.
31211931	11	22	theme	colon	2059:2063	arg1	epithelium					2065:2074	injured mouse colon epithelium	2045:2074	injured mouse colon epithelium	2045:2074	In vivo evaluation of scaffolds compatible for colonoid engraftments onto injured mouse colon epithelium.
31211931	9	23	theme	clinical	1630:1637	arg1	application					1639:1649	the clinical application	1626:1649	the clinical application of colonoid-based therapeutics	1626:1680	This study suggests new methods for and provides evidence of the safety and utility of the clinical application of colonoid-based therapeutics.
31211931	0	24	theme	In	0:1	arg1	evaluation					8:17	In vivo evaluation	0:17	In vivo evaluation of scaffolds compatible for colonoid	0:54	In vivo evaluation of scaffolds compatible for colonoid engraftments onto injured mouse colon epithelium.
31211931	4	25	theme	protein	669:675	arg1	mice					698:701	green fluorescent protein (EGFP)131Osb/LeySopJ mice	651:701	green fluorescent protein (EGFP)131Osb/LeySopJ mice into EGFP + colonoids	651:723	The colon crypt was isolated and cultured from C57BL/6-Tg[CAG enhanced green fluorescent protein (EGFP)131Osb/LeySopJ mice into EGFP + colonoids and subsequently transplanted into the EDTA colitis mouse model using gelatin, collagen, or fibrin glue scaffolds.
31211931	10	26	theme	cell	1763:1766	arg1	treatment					1768:1776	cell treatment	1763:1776	cell treatment using the esophagus or a stomach organoid for various digestive-system diseases.-Jee, J., Jeong, S. Y., Kim, H. K., Choi, S. Y., Jeong, S., Lee, J., Ko, J. S., Kim, M. S., Kwon, M.-S., Yoo, J	1763:1968	Furthermore, the methods introduced in this study will be helpful in developing cell treatment using the esophagus or a stomach organoid for various digestive-system diseases.-Jee, J., Jeong, S. Y., Kim, H. K., Choi, S. Y., Jeong, S., Lee, J., Ko, J. S., Kim, M. S., Kwon, M.-S., Yoo, J.
31211931	9	27	theme	application	1639:1649	arg1	utility					1615:1621	utility	1615:1621	utility	1615:1621	This study suggests new methods for and provides evidence of the safety and utility of the clinical application of colonoid-based therapeutics.
31211931	9	27	theme	application	1639:1649	arg1	safety					1604:1609	safety	1604:1609	safety	1604:1609	This study suggests new methods for and provides evidence of the safety and utility of the clinical application of colonoid-based therapeutics.
31211931	2	28	used	used	302:305	arg2	Matrigel					283:290	Matrigel	283:290	Matrigel	283:290	Matrigel, which is used as a transplantation scaffold for colonoids, cannot be used in clinical applications because of its undefined composition and tumorigenicity.
31211931	2	28	used	used	302:305	arg2	scaffold					328:335	a transplantation scaffold	310:335	a transplantation scaffold for colonoids	310:349	Matrigel, which is used as a transplantation scaffold for colonoids, cannot be used in clinical applications because of its undefined composition and tumorigenicity.
31211931	1	29	theme	colon	161:165	arg1	tissue					167:172	colon tissue	161:172	colon tissue in structure and function, which makes them useful in the treatment of intestinal de-epithelialized disease	161:280	Colon organoids (colonoids) are known to be similar to colon tissue in structure and function, which makes them useful in the treatment of intestinal de-epithelialized disease.
31211931	2	30	theme	undefined	407:415	arg1	composition					417:427	its undefined composition	403:427	its undefined composition	403:427	Matrigel, which is used as a transplantation scaffold for colonoids, cannot be used in clinical applications because of its undefined composition and tumorigenicity.
31211931	5	31	theme	transplantation	942:956	arg1	rates					933:937	the success rates	921:937	the success rates of transplantation and secondary EGFP colonoid formation	921:994	To identify scaffolds suitable for colonoid engraftment in injured colon mucosa, the success rates of transplantation and secondary EGFP colonoid formation were measured, and the scaffolds' mediated toxicity in vitro and in vivo was observed in recipient mice.
31211931	4	32	dep	gelatin	795:801	arg1	scaffolds					829:837	glue scaffolds	824:837	glue scaffolds	824:837	The colon crypt was isolated and cultured from C57BL/6-Tg[CAG enhanced green fluorescent protein (EGFP)131Osb/LeySopJ mice into EGFP + colonoids and subsequently transplanted into the EDTA colitis mouse model using gelatin, collagen, or fibrin glue scaffolds.
31211931	1	33	theme	intestinal	245:254	arg1	disease					274:280	intestinal de-epithelialized disease	245:280	intestinal de-epithelialized disease	245:280	Colon organoids (colonoids) are known to be similar to colon tissue in structure and function, which makes them useful in the treatment of intestinal de-epithelialized disease.
31211931	9	34	theme	colonoid-based	1654:1667	arg1	therapeutics					1669:1680	colonoid-based therapeutics	1654:1680	colonoid-based therapeutics	1654:1680	This study suggests new methods for and provides evidence of the safety and utility of the clinical application of colonoid-based therapeutics.
31211931	4	35	dep	C57BL/6-Tg[CAG	627:640	arg1	transplanted					742:753	transplanted	742:753	subsequently transplanted into the EDTA colitis mouse model using gelatin, collagen, or fibrin glue scaffolds	729:837	The colon crypt was isolated and cultured from C57BL/6-Tg[CAG enhanced green fluorescent protein (EGFP)131Osb/LeySopJ mice into EGFP + colonoids and subsequently transplanted into the EDTA colitis mouse model using gelatin, collagen, or fibrin glue scaffolds.
31211931	4	35	dep	C57BL/6-Tg[CAG	627:640	arg1	enhanced					642:649	enhanced	642:649	enhanced green fluorescent protein (EGFP)131Osb/LeySopJ mice into EGFP + colonoids	642:723	The colon crypt was isolated and cultured from C57BL/6-Tg[CAG enhanced green fluorescent protein (EGFP)131Osb/LeySopJ mice into EGFP + colonoids and subsequently transplanted into the EDTA colitis mouse model using gelatin, collagen, or fibrin glue scaffolds.
31211931	3	36	theme	damaged	553:559	arg1	mucosa					572:577	damaged intestinal mucosa	553:577	damaged intestinal mucosa	553:577	This study identifies clinically available scaffolds that are effective for colonoid transplantation in damaged intestinal mucosa.
31211931	9	37	theme	new	1559:1561	arg1	methods					1563:1569	new methods	1559:1569	new methods for	1559:1573	This study suggests new methods for and provides evidence of the safety and utility of the clinical application of colonoid-based therapeutics.
31211931	5	38	from	engraftment	884:894	arg1	mucosa					913:918	injured colon mucosa	899:918	injured colon mucosa	899:918	To identify scaffolds suitable for colonoid engraftment in injured colon mucosa, the success rates of transplantation and secondary EGFP colonoid formation were measured, and the scaffolds' mediated toxicity in vitro and in vivo was observed in recipient mice.
31211931	1	39	theme	de-epithelialized	256:272	arg1	disease					274:280	intestinal de-epithelialized disease	245:280	intestinal de-epithelialized disease	245:280	Colon organoids (colonoids) are known to be similar to colon tissue in structure and function, which makes them useful in the treatment of intestinal de-epithelialized disease.
31211931	1	40	from	treatment	232:240	arg1	useful					218:223	useful	218:223	useful	218:223	Colon organoids (colonoids) are known to be similar to colon tissue in structure and function, which makes them useful in the treatment of intestinal de-epithelialized disease.
31211931	1	41	theme	Colon	106:110	arg1	colonoids					123:131	colonoids	123:131	colonoids	123:131	Colon organoids (colonoids) are known to be similar to colon tissue in structure and function, which makes them useful in the treatment of intestinal de-epithelialized disease.
31211931	1	41	theme	Colon	106:110	arg1	organoids					112:120	Colon organoids	106:120	Colon organoids (colonoids)	106:132	Colon organoids (colonoids) are known to be similar to colon tissue in structure and function, which makes them useful in the treatment of intestinal de-epithelialized disease.
31211931	0	42	theme	compatible	32:41	arg1	scaffolds					22:30	scaffolds	22:30	scaffolds compatible for colonoid	22:54	In vivo evaluation of scaffolds compatible for colonoid engraftments onto injured mouse colon epithelium.
31211931	1	43	theme	disease	274:280	arg1	treatment					232:240	the treatment	228:240	the treatment of intestinal de-epithelialized disease	228:280	Colon organoids (colonoids) are known to be similar to colon tissue in structure and function, which makes them useful in the treatment of intestinal de-epithelialized disease.
31211931	1	44	from	useful	218:223	arg1	treatment					232:240	the treatment	228:240	the treatment of intestinal de-epithelialized disease	228:280	Colon organoids (colonoids) are known to be similar to colon tissue in structure and function, which makes them useful in the treatment of intestinal de-epithelialized disease.
31211931	4	45	theme	fluorescent	657:667	arg1	EGFP					678:681	EGFP	678:681	EGFP	678:681	The colon crypt was isolated and cultured from C57BL/6-Tg[CAG enhanced green fluorescent protein (EGFP)131Osb/LeySopJ mice into EGFP + colonoids and subsequently transplanted into the EDTA colitis mouse model using gelatin, collagen, or fibrin glue scaffolds.
31211931	4	45	theme	fluorescent	657:667	arg1	protein					669:675	green fluorescent protein	651:675	green fluorescent protein (EGFP)131Osb/LeySopJ mice into EGFP + colonoids	651:723	The colon crypt was isolated and cultured from C57BL/6-Tg[CAG enhanced green fluorescent protein (EGFP)131Osb/LeySopJ mice into EGFP + colonoids and subsequently transplanted into the EDTA colitis mouse model using gelatin, collagen, or fibrin glue scaffolds.
31211931	7	46	dep	showed	1286:1291	arg1	compared					1336:1343	compared	1336:1343	showed significant increase in the engrafted area compared with Matrigel after 4 wk	1286:1368	Fibrin glue, especially, showed significant increase in the engrafted area compared with Matrigel after 4 wk.
31211931	5	47	located	observed	1073:1080	arg2	toxicity					1039:1046	the scaffolds' mediated toxicity	1015:1046	the scaffolds' mediated toxicity in vitro and in vivo	1015:1067	To identify scaffolds suitable for colonoid engraftment in injured colon mucosa, the success rates of transplantation and secondary EGFP colonoid formation were measured, and the scaffolds' mediated toxicity in vitro and in vivo was observed in recipient mice.
31211931	5	47	located	observed	1073:1080	arg1	mice					1095:1098	recipient mice	1085:1098	recipient mice	1085:1098	To identify scaffolds suitable for colonoid engraftment in injured colon mucosa, the success rates of transplantation and secondary EGFP colonoid formation were measured, and the scaffolds' mediated toxicity in vitro and in vivo was observed in recipient mice.
31211931	2	48	theme	clinical	370:377	arg1	applications					379:390	clinical applications	370:390	clinical applications	370:390	Matrigel, which is used as a transplantation scaffold for colonoids, cannot be used in clinical applications because of its undefined composition and tumorigenicity.
31211931	0	49	theme	scaffolds	22:30	arg1	evaluation					8:17	In vivo evaluation	0:17	In vivo evaluation of scaffolds compatible for colonoid	0:54	In vivo evaluation of scaffolds compatible for colonoid engraftments onto injured mouse colon epithelium.
31211931	8	50	theme	colonic	1418:1424	arg1	toxicity					1426:1433	colonic toxicity	1418:1433	colonic toxicity	1418:1433	The scaffolds used in the study did not induce colonic toxicity after transplantation into the recipients' colons and were thus deemed safe when locally administrated.
31211931	4	51	theme	green	651:655	arg1	EGFP					678:681	EGFP	678:681	EGFP	678:681	The colon crypt was isolated and cultured from C57BL/6-Tg[CAG enhanced green fluorescent protein (EGFP)131Osb/LeySopJ mice into EGFP + colonoids and subsequently transplanted into the EDTA colitis mouse model using gelatin, collagen, or fibrin glue scaffolds.
31211931	4	51	theme	green	651:655	arg1	protein					669:675	green fluorescent protein	651:675	green fluorescent protein (EGFP)131Osb/LeySopJ mice into EGFP + colonoids	651:723	The colon crypt was isolated and cultured from C57BL/6-Tg[CAG enhanced green fluorescent protein (EGFP)131Osb/LeySopJ mice into EGFP + colonoids and subsequently transplanted into the EDTA colitis mouse model using gelatin, collagen, or fibrin glue scaffolds.
31211931	11	52	theme	In	1971:1972	arg1	evaluation					1979:1988	In vivo evaluation	1971:1988	In vivo evaluation of scaffolds compatible for colonoid	1971:2025	In vivo evaluation of scaffolds compatible for colonoid engraftments onto injured mouse colon epithelium.
31211931	5	53	theme	mediated	1030:1037	arg1	toxicity					1039:1046	the scaffolds' mediated toxicity	1015:1046	the scaffolds' mediated toxicity in vitro and in vivo	1015:1067	To identify scaffolds suitable for colonoid engraftment in injured colon mucosa, the success rates of transplantation and secondary EGFP colonoid formation were measured, and the scaffolds' mediated toxicity in vitro and in vivo was observed in recipient mice.
31211931	10	54	theme	various	1824:1830	arg1	digestive-system					1832:1847	various digestive-system diseases.-Jee, J., Jeong, S. Y., Kim, H. K., Choi, S. Y., Jeong, S., Lee, J., Ko, J. S., Kim, M. S., Kwon, M.-S., Yoo, J	1824:1968	digestive-system	1832:1847	Furthermore, the methods introduced in this study will be helpful in developing cell treatment using the esophagus or a stomach organoid for various digestive-system diseases.-Jee, J., Jeong, S. Y., Kim, H. K., Choi, S. Y., Jeong, S., Lee, J., Ko, J. S., Kim, M. S., Kwon, M.-S., Yoo, J.
31211931	10	54	theme	various	1824:1830	arg1	J					1968:1968	J	1968:1968	J	1968:1968	Furthermore, the methods introduced in this study will be helpful in developing cell treatment using the esophagus or a stomach organoid for various digestive-system diseases.-Jee, J., Jeong, S. Y., Kim, H. K., Choi, S. Y., Jeong, S., Lee, J., Ko, J. S., Kim, M. S., Kwon, M.-S., Yoo, J.
31211931	11	55	theme	scaffolds	1993:2001	arg1	evaluation					1979:1988	In vivo evaluation	1971:1988	In vivo evaluation of scaffolds compatible for colonoid	1971:2025	In vivo evaluation of scaffolds compatible for colonoid engraftments onto injured mouse colon epithelium.
31211931	7	56	theme	significant	1293:1303	arg1	increase					1305:1312	significant increase	1293:1312	significant increase in the engrafted area	1293:1334	Fibrin glue, especially, showed significant increase in the engrafted area compared with Matrigel after 4 wk.
31211931	4	57	theme	glue	824:827	arg1	scaffolds					829:837	glue scaffolds	824:837	glue scaffolds	824:837	The colon crypt was isolated and cultured from C57BL/6-Tg[CAG enhanced green fluorescent protein (EGFP)131Osb/LeySopJ mice into EGFP + colonoids and subsequently transplanted into the EDTA colitis mouse model using gelatin, collagen, or fibrin glue scaffolds.
31211931	3	58	theme	available	482:490	arg1	scaffolds					492:500	clinically available scaffolds	471:500	clinically available scaffolds that are effective for colonoid transplantation in damaged intestinal mucosa	471:577	This study identifies clinically available scaffolds that are effective for colonoid transplantation in damaged intestinal mucosa.
31211931	7	59	theme	engrafted	1321:1329	arg1	area					1331:1334	the engrafted area	1317:1334	the engrafted area	1317:1334	Fibrin glue, especially, showed significant increase in the engrafted area compared with Matrigel after 4 wk.
31211931	10	60	theme	stomach	1803:1809	arg1	organoid					1811:1818	a stomach organoid	1801:1818	a stomach organoid for various digestive-system diseases.-Jee, J., Jeong, S. Y., Kim, H. K., Choi, S. Y., Jeong, S., Lee, J., Ko, J. S., Kim, M. S., Kwon, M.-S., Yoo, J	1801:1968	Furthermore, the methods introduced in this study will be helpful in developing cell treatment using the esophagus or a stomach organoid for various digestive-system diseases.-Jee, J., Jeong, S. Y., Kim, H. K., Choi, S. Y., Jeong, S., Lee, J., Ko, J. S., Kim, M. S., Kwon, M.-S., Yoo, J.
31211931	6	61	theme	mouse	1196:1200	arg1	model					1202:1206	the EDTA colitis mouse model	1179:1206	the EDTA colitis mouse model	1179:1206	When colonoids were transplanted with gelatin, collagen, and fibrin glue into the EDTA colitis mouse model, all groups were found to be successfully engrafted.
31211931	11	62	theme	compatible	2003:2012	arg1	scaffolds					1993:2001	scaffolds	1993:2001	scaffolds compatible for colonoid	1993:2025	In vivo evaluation of scaffolds compatible for colonoid engraftments onto injured mouse colon epithelium.
31211931	5	63	theme	success	925:931	arg1	rates					933:937	the success rates	921:937	the success rates of transplantation and secondary EGFP colonoid formation	921:994	To identify scaffolds suitable for colonoid engraftment in injured colon mucosa, the success rates of transplantation and secondary EGFP colonoid formation were measured, and the scaffolds' mediated toxicity in vitro and in vivo was observed in recipient mice.
31211931	4	64	theme	EDTA	764:767	arg1	model					783:787	the EDTA colitis mouse model	760:787	the EDTA colitis mouse model using gelatin, collagen, or fibrin glue scaffolds	760:837	The colon crypt was isolated and cultured from C57BL/6-Tg[CAG enhanced green fluorescent protein (EGFP)131Osb/LeySopJ mice into EGFP + colonoids and subsequently transplanted into the EDTA colitis mouse model using gelatin, collagen, or fibrin glue scaffolds.
31211931	3	65	theme	colonoid	525:532	arg1	transplantation					534:548	colonoid transplantation	525:548	colonoid transplantation in damaged intestinal mucosa	525:577	This study identifies clinically available scaffolds that are effective for colonoid transplantation in damaged intestinal mucosa.
31211931	7	66	theme	Fibrin	1261:1266	arg1	glue					1268:1271	Fibrin glue	1261:1271	Fibrin glue	1261:1271	Fibrin glue, especially, showed significant increase in the engrafted area compared with Matrigel after 4 wk.
31211931	5	67	theme	EGFP	972:975	arg1	formation					986:994	secondary EGFP colonoid formation	962:994	secondary EGFP colonoid formation	962:994	To identify scaffolds suitable for colonoid engraftment in injured colon mucosa, the success rates of transplantation and secondary EGFP colonoid formation were measured, and the scaffolds' mediated toxicity in vitro and in vivo was observed in recipient mice.
31211931	5	68	theme	injured	899:905	arg1	mucosa					913:918	injured colon mucosa	899:918	injured colon mucosa	899:918	To identify scaffolds suitable for colonoid engraftment in injured colon mucosa, the success rates of transplantation and secondary EGFP colonoid formation were measured, and the scaffolds' mediated toxicity in vitro and in vivo was observed in recipient mice.
31211931	10	69	theme	diseases.-Jee	1849:1861	arg1	digestive-system					1832:1847	various digestive-system diseases.-Jee, J., Jeong, S. Y., Kim, H. K., Choi, S. Y., Jeong, S., Lee, J., Ko, J. S., Kim, M. S., Kwon, M.-S., Yoo, J	1824:1968	digestive-system	1832:1847	Furthermore, the methods introduced in this study will be helpful in developing cell treatment using the esophagus or a stomach organoid for various digestive-system diseases.-Jee, J., Jeong, S. Y., Kim, H. K., Choi, S. Y., Jeong, S., Lee, J., Ko, J. S., Kim, M. S., Kwon, M.-S., Yoo, J.
31211931	10	69	theme	diseases.-Jee	1849:1861	arg1	J					1968:1968	J	1968:1968	J	1968:1968	Furthermore, the methods introduced in this study will be helpful in developing cell treatment using the esophagus or a stomach organoid for various digestive-system diseases.-Jee, J., Jeong, S. Y., Kim, H. K., Choi, S. Y., Jeong, S., Lee, J., Ko, J. S., Kim, M. S., Kwon, M.-S., Yoo, J.
31211931	6	70	theme	EDTA	1183:1186	arg1	model					1202:1206	the EDTA colitis mouse model	1179:1206	the EDTA colitis mouse model	1179:1206	When colonoids were transplanted with gelatin, collagen, and fibrin glue into the EDTA colitis mouse model, all groups were found to be successfully engrafted.
31211931	1	71	from	tissue	167:172	arg1	structure					177:185	structure	177:185	structure	177:185	Colon organoids (colonoids) are known to be similar to colon tissue in structure and function, which makes them useful in the treatment of intestinal de-epithelialized disease.
31211931	1	71	from	tissue	167:172	arg1	function					191:198	function	191:198	function	191:198	Colon organoids (colonoids) are known to be similar to colon tissue in structure and function, which makes them useful in the treatment of intestinal de-epithelialized disease.
31211931	5	72	theme	secondary	962:970	arg1	formation					986:994	secondary EGFP colonoid formation	962:994	secondary EGFP colonoid formation	962:994	To identify scaffolds suitable for colonoid engraftment in injured colon mucosa, the success rates of transplantation and secondary EGFP colonoid formation were measured, and the scaffolds' mediated toxicity in vitro and in vivo was observed in recipient mice.
31211931	6	73	dep	gelatin	1139:1145	arg1	glue					1169:1172	glue	1169:1172	glue	1169:1172	When colonoids were transplanted with gelatin, collagen, and fibrin glue into the EDTA colitis mouse model, all groups were found to be successfully engrafted.
31211931	11	74	theme	injured	2045:2051	arg1	epithelium					2065:2074	injured mouse colon epithelium	2045:2074	injured mouse colon epithelium	2045:2074	In vivo evaluation of scaffolds compatible for colonoid engraftments onto injured mouse colon epithelium.
31211931	0	75	dep	In	0:1	arg1	vivo					3:6	vivo	3:6	vivo	3:6	In vivo evaluation of scaffolds compatible for colonoid engraftments onto injured mouse colon epithelium.
31211931	7	76	from	increase	1305:1312	arg1	area					1331:1334	the engrafted area	1317:1334	the engrafted area	1317:1334	Fibrin glue, especially, showed significant increase in the engrafted area compared with Matrigel after 4 wk.
31211931	4	77	theme	131Osb/LeySopJ	683:696	arg1	mice					698:701	green fluorescent protein (EGFP)131Osb/LeySopJ mice	651:701	green fluorescent protein (EGFP)131Osb/LeySopJ mice into EGFP + colonoids	651:723	The colon crypt was isolated and cultured from C57BL/6-Tg[CAG enhanced green fluorescent protein (EGFP)131Osb/LeySopJ mice into EGFP + colonoids and subsequently transplanted into the EDTA colitis mouse model using gelatin, collagen, or fibrin glue scaffolds.
31211931	9	78	theme	therapeutics	1669:1680	arg1	application					1639:1649	the clinical application	1626:1649	the clinical application of colonoid-based therapeutics	1626:1680	This study suggests new methods for and provides evidence of the safety and utility of the clinical application of colonoid-based therapeutics.
30444470	4	0	theme	Strain	312:317	arg1	CHM3-46T					319:326	Strain CHM3-46T	312:326	Strain CHM3-46T	312:326	Strain CHM3-46T produced short, straight chains of warty spores on aerial mycelia.
30444470	11	1	theme	Thermocatellispora	1187:1204	arg1	77521T					1225:1230	Thermocatellispora tengchongensis YIM 77521T	1187:1230	Thermocatellispora tengchongensis YIM 77521T (98.5 %)	1187:1239	Analysis of the 16S rRNA gene sequence showed that strain CHM3-46T belonged to the genus Thermocatellispora, exhibiting the highest similarity to Thermocatellispora tengchongensis YIM 77521T (98.5 %).
30444470	11	1	theme	Thermocatellispora	1187:1204	arg1	%					1238:1238	98.5 %	1233:1238	98.5 %	1233:1238	Analysis of the 16S rRNA gene sequence showed that strain CHM3-46T belonged to the genus Thermocatellispora, exhibiting the highest similarity to Thermocatellispora tengchongensis YIM 77521T (98.5 %).
30444470	4	2	theme	aerial	379:384	arg1	mycelia					386:392	aerial mycelia	379:392	aerial mycelia	379:392	Strain CHM3-46T produced short, straight chains of warty spores on aerial mycelia.
30444470	9	3	theme	10-methyl	889:897	arg1	C17 					899:902	10-methyl C17 	889:902	10-methyl C17 : 0, C16 : 0, C17 : 0 and iso-C16 : 0 were identified as the main cellular fatty acids.	889:989	10-methyl C17 : 0, C16 : 0, C17 : 0 and iso-C16 : 0 were identified as the main cellular fatty acids.
30444470	1	4	theme	hot	48:50	arg1	soil					59:62	hot spring soil	48:62	hot spring soil	48:62	nov., isolated from hot spring soil.
30444470	5	5	theme	meso-diaminopimelic	411:429	arg1	acid					431:434	meso-diaminopimelic acid	411:434	meso-diaminopimelic acid	411:434	The presence of meso-diaminopimelic acid was observed in the cell-wall peptidoglycan.
30444470	5	6	located	observed	440:447	arg1	peptidoglycan					466:478	the cell-wall peptidoglycan	452:478	the cell-wall peptidoglycan	452:478	The presence of meso-diaminopimelic acid was observed in the cell-wall peptidoglycan.
30444470	5	6	located	observed	440:447	arg2	presence					399:406	The presence	395:406	The presence of meso-diaminopimelic acid	395:434	The presence of meso-diaminopimelic acid was observed in the cell-wall peptidoglycan.
30444470	1	7	theme	spring	52:57	arg1	soil					59:62	hot spring soil	48:62	hot spring soil	48:62	nov., isolated from hot spring soil.
30444470	9	8	theme	main	964:967	arg1	acids					984:988	the main cellular fatty acids	960:988	the main cellular fatty acids	960:988	10-methyl C17 : 0, C16 : 0, C17 : 0 and iso-C16 : 0 were identified as the main cellular fatty acids.
30444470	9	8	theme	main	964:967	arg1	C16 					908:911	C16 	908:911	C16 	908:911	10-methyl C17 : 0, C16 : 0, C17 : 0 and iso-C16 : 0 were identified as the main cellular fatty acids.
30444470	13	9	theme	novel	1561:1565	arg1	species					1567:1573	a novel species	1559:1573	a novel species	1559:1573	Based on the taxonomic data, strain CHM3-46T could be readily distinguished from its closest phylogenetic relative and represents a novel species, for which the name Thermocatellisporasoli sp.
30444470	11	10	theme	highest	1165:1171	arg1	similarity					1173:1182	the highest similarity	1161:1182	the highest similarity to Thermocatellispora tengchongensis YIM 77521T (98.5 %)	1161:1239	Analysis of the 16S rRNA gene sequence showed that strain CHM3-46T belonged to the genus Thermocatellispora, exhibiting the highest similarity to Thermocatellispora tengchongensis YIM 77521T (98.5 %).
30444470	2	11	from	located	178:184	arg1	Thailand					209:216	Thailand	209:216	Thailand	209:216	An aerobic, spore-forming, actinomycete, designated strain CHM3-46T, was isolated from soil in a hot spring pond located in Chiangmai province, Thailand.
30444470	3	12	with	consistent	266:275	arg1	Thermocatellispora					292:309	the genus Thermocatellispora	282:309	the genus Thermocatellispora	282:309	The strain exhibited taxonomic characteristics consistent with the genus Thermocatellispora.
30444470	9	13	theme	cellular	969:976	arg1	acids					984:988	the main cellular fatty acids	960:988	the main cellular fatty acids	960:988	10-methyl C17 : 0, C16 : 0, C17 : 0 and iso-C16 : 0 were identified as the main cellular fatty acids.
30444470	9	13	theme	cellular	969:976	arg1	C16 					908:911	C16 	908:911	C16 	908:911	10-methyl C17 : 0, C16 : 0, C17 : 0 and iso-C16 : 0 were identified as the main cellular fatty acids.
30444470	13	14	theme	closest	1514:1520	arg1	relative					1535:1542	its closest phylogenetic relative	1510:1542	its closest phylogenetic relative	1510:1542	Based on the taxonomic data, strain CHM3-46T could be readily distinguished from its closest phylogenetic relative and represents a novel species, for which the name Thermocatellisporasoli sp.
30444470	11	15	theme	YIM	1221:1223	arg1	77521T					1225:1230	Thermocatellispora tengchongensis YIM 77521T	1187:1230	Thermocatellispora tengchongensis YIM 77521T (98.5 %)	1187:1239	Analysis of the 16S rRNA gene sequence showed that strain CHM3-46T belonged to the genus Thermocatellispora, exhibiting the highest similarity to Thermocatellispora tengchongensis YIM 77521T (98.5 %).
30444470	11	15	theme	YIM	1221:1223	arg1	%					1238:1238	98.5 %	1233:1238	98.5 %	1233:1238	Analysis of the 16S rRNA gene sequence showed that strain CHM3-46T belonged to the genus Thermocatellispora, exhibiting the highest similarity to Thermocatellispora tengchongensis YIM 77521T (98.5 %).
30444470	9	16	dep	C17 	899:902	arg1	identified					946:955	identified	946:955	were identified as the main cellular fatty acids	941:988	10-methyl C17 : 0, C16 : 0, C17 : 0 and iso-C16 : 0 were identified as the main cellular fatty acids.
30444470	11	17	theme	sequence	1071:1078	arg1	Analysis					1041:1048	Analysis	1041:1048	Analysis of the 16S rRNA gene sequence	1041:1078	Analysis of the 16S rRNA gene sequence showed that strain CHM3-46T belonged to the genus Thermocatellispora, exhibiting the highest similarity to Thermocatellispora tengchongensis YIM 77521T (98.5 %).
30444470	0	18	theme	soli	19:22	arg1	sp					24:25	Thermocatellispora soli sp	0:25	Thermocatellispora soli sp.	0:26	Thermocatellispora soli sp.
30444470	2	19	theme	strain	117:122	arg1	CHM3-46T					124:131	strain CHM3-46T	117:131	strain CHM3-46T	117:131	An aerobic, spore-forming, actinomycete, designated strain CHM3-46T, was isolated from soil in a hot spring pond located in Chiangmai province, Thailand.
30444470	11	20	theme	16S	1057:1059	arg1	rRNA					1061:1064	16S rRNA	1057:1064	the 16S rRNA gene sequence	1053:1078	Analysis of the 16S rRNA gene sequence showed that strain CHM3-46T belonged to the genus Thermocatellispora, exhibiting the highest similarity to Thermocatellispora tengchongensis YIM 77521T (98.5 %).
30444470	0	21	theme	Thermocatellispora	0:17	arg1	sp					24:25	Thermocatellispora soli sp	0:25	Thermocatellispora soli sp.	0:26	Thermocatellispora soli sp.
30444470	13	22	theme	name	1590:1593	arg1	sp					1618:1619	the name Thermocatellisporasoli sp	1586:1619	the name Thermocatellisporasoli sp	1586:1619	Based on the taxonomic data, strain CHM3-46T could be readily distinguished from its closest phylogenetic relative and represents a novel species, for which the name Thermocatellisporasoli sp.
30444470	4	23	theme	spores	369:374	arg1	chains					353:358	short, straight chains	337:358	short, straight chains of warty spores on aerial mycelia	337:392	Strain CHM3-46T produced short, straight chains of warty spores on aerial mycelia.
30444470	13	24	theme	phylogenetic	1522:1533	arg1	relative					1535:1542	its closest phylogenetic relative	1510:1542	its closest phylogenetic relative	1510:1542	Based on the taxonomic data, strain CHM3-46T could be readily distinguished from its closest phylogenetic relative and represents a novel species, for which the name Thermocatellisporasoli sp.
30444470	2	25	from	Thailand	209:216	arg1	located					178:184	located	178:184	located	178:184	An aerobic, spore-forming, actinomycete, designated strain CHM3-46T, was isolated from soil in a hot spring pond located in Chiangmai province, Thailand.
30444470	11	26	theme	rRNA	1061:1064	arg1	sequence					1071:1078	the 16S rRNA gene sequence	1053:1078	the 16S rRNA gene sequence	1053:1078	Analysis of the 16S rRNA gene sequence showed that strain CHM3-46T belonged to the genus Thermocatellispora, exhibiting the highest similarity to Thermocatellispora tengchongensis YIM 77521T (98.5 %).
30444470	8	27	theme	predominant	826:836	arg1	MK-9					856:859	MK-9	856:859	MK-9	856:859	The predominant menaquinones were MK-9(H4), MK-9(H6) and MK-9(H8).
30444470	8	27	theme	predominant	826:836	arg1	menaquinones					838:849	The predominant menaquinones	822:849	The predominant menaquinones	822:849	The predominant menaquinones were MK-9(H4), MK-9(H6) and MK-9(H8).
30444470	3	28	theme	taxonomic	240:248	arg1	characteristics					250:264	taxonomic characteristics	240:264	taxonomic characteristics consistent with the genus Thermocatellispora	240:309	The strain exhibited taxonomic characteristics consistent with the genus Thermocatellispora.
30444470	4	29	theme	warty	363:367	arg1	spores					369:374	warty spores	363:374	warty spores	363:374	Strain CHM3-46T produced short, straight chains of warty spores on aerial mycelia.
30444470	11	30	theme	tengchongensis	1206:1219	arg1	77521T					1225:1230	Thermocatellispora tengchongensis YIM 77521T	1187:1230	Thermocatellispora tengchongensis YIM 77521T (98.5 %)	1187:1239	Analysis of the 16S rRNA gene sequence showed that strain CHM3-46T belonged to the genus Thermocatellispora, exhibiting the highest similarity to Thermocatellispora tengchongensis YIM 77521T (98.5 %).
30444470	11	30	theme	tengchongensis	1206:1219	arg1	%					1238:1238	98.5 %	1233:1238	98.5 %	1233:1238	Analysis of the 16S rRNA gene sequence showed that strain CHM3-46T belonged to the genus Thermocatellispora, exhibiting the highest similarity to Thermocatellispora tengchongensis YIM 77521T (98.5 %).
30444470	5	31	theme	cell-wall	456:464	arg1	peptidoglycan					466:478	the cell-wall peptidoglycan	452:478	the cell-wall peptidoglycan	452:478	The presence of meso-diaminopimelic acid was observed in the cell-wall peptidoglycan.
30444470	11	32	theme	strain	1092:1097	arg1	CHM3-46T					1099:1106	strain CHM3-46T	1092:1106	strain CHM3-46T	1092:1106	Analysis of the 16S rRNA gene sequence showed that strain CHM3-46T belonged to the genus Thermocatellispora, exhibiting the highest similarity to Thermocatellispora tengchongensis YIM 77521T (98.5 %).
30444470	15	33	theme	7649T=NBRC	1675:1684	arg1	113148T					1686:1692	=TBRC 7649T=NBRC 113148T	1669:1692	=TBRC 7649T=NBRC 113148T	1669:1692	The type strain is CHM3-46T (=TBRC 7649T=NBRC 113148T).
30444470	15	33	theme	7649T=NBRC	1675:1684	arg1	CHM3-46T					1659:1666	CHM3-46T	1659:1666	CHM3-46T (=TBRC 7649T=NBRC 113148T)	1659:1693	The type strain is CHM3-46T (=TBRC 7649T=NBRC 113148T).
30444470	6	34	theme	reducing	496:503	arg1	glucose					517:523	glucose	517:523	glucose	517:523	The whole-cell reducing sugars were glucose, mannose, galacose and ribose.
30444470	6	34	theme	reducing	496:503	arg1	sugars					505:510	The whole-cell reducing sugars	481:510	The whole-cell reducing sugars	481:510	The whole-cell reducing sugars were glucose, mannose, galacose and ribose.
30444470	4	35	from	chains	353:358	arg1	mycelia					386:392	aerial mycelia	379:392	aerial mycelia	379:392	Strain CHM3-46T produced short, straight chains of warty spores on aerial mycelia.
30444470	4	36	dep	short	337:341	arg1	straight					344:351	straight	344:351	straight	344:351	Strain CHM3-46T produced short, straight chains of warty spores on aerial mycelia.
30444470	12	37	theme	DNA	1261:1263	arg1	value					1277:1281	a low DNA relatedness value	1255:1281	a low DNA relatedness value (23.4 %±0.8)	1255:1294	Furthermore, a low DNA relatedness value (23.4 %±0.8) and several physiological and biochemical characteristic differences were detected between strain CHM3-46T and its closest relative.
30444470	12	37	theme	DNA	1261:1263	arg1	%					1289:1289	23.4 %±0.8	1284:1293	23.4 %±0.8	1284:1293	Furthermore, a low DNA relatedness value (23.4 %±0.8) and several physiological and biochemical characteristic differences were detected between strain CHM3-46T and its closest relative.
30444470	6	38	theme	whole-cell	485:494	arg1	glucose					517:523	glucose	517:523	glucose	517:523	The whole-cell reducing sugars were glucose, mannose, galacose and ribose.
30444470	6	38	theme	whole-cell	485:494	arg1	sugars					505:510	The whole-cell reducing sugars	481:510	The whole-cell reducing sugars	481:510	The whole-cell reducing sugars were glucose, mannose, galacose and ribose.
30444470	10	39	theme	73.2 mol	1030:1037	arg1	%					1038:1038	73.2 mol%	1030:1038	73.2 mol%	1030:1038	The G+C content of the genomic DNA was 73.2 mol%.
30444470	10	39	theme	73.2 mol	1030:1037	arg1	G+C content					995:1005	The G+C content	991:1005	The G+C content of the genomic DNA	991:1024	The G+C content of the genomic DNA was 73.2 mol%.
30444470	12	40	theme	characteristic	1338:1351	arg1	differences					1353:1363	several physiological and biochemical characteristic differences	1300:1363	several physiological and biochemical characteristic differences	1300:1363	Furthermore, a low DNA relatedness value (23.4 %±0.8) and several physiological and biochemical characteristic differences were detected between strain CHM3-46T and its closest relative.
30444470	11	41	theme	gene	1066:1069	arg1	sequence					1071:1078	the 16S rRNA gene sequence	1053:1078	the 16S rRNA gene sequence	1053:1078	Analysis of the 16S rRNA gene sequence showed that strain CHM3-46T belonged to the genus Thermocatellispora, exhibiting the highest similarity to Thermocatellispora tengchongensis YIM 77521T (98.5 %).
30444470	2	42	attach	isolated	138:145	arg1	soil					152:155	soil	152:155	soil	152:155	An aerobic, spore-forming, actinomycete, designated strain CHM3-46T, was isolated from soil in a hot spring pond located in Chiangmai province, Thailand.
30444470	2	42	attach	isolated	138:145	arg2	actinomycete					92:103	An aerobic, spore-forming, actinomycete	65:103	actinomycete	92:103	An aerobic, spore-forming, actinomycete, designated strain CHM3-46T, was isolated from soil in a hot spring pond located in Chiangmai province, Thailand.
30444470	9	43	dep	C16 	908:911	arg1	 0					922:923	 0	922:923	 0	922:923	10-methyl C17 : 0, C16 : 0, C17 : 0 and iso-C16 : 0 were identified as the main cellular fatty acids.
30444470	9	43	dep	C16 	908:911	arg1	C17 					917:920	C17 	917:920	C17 	917:920	10-methyl C17 : 0, C16 : 0, C17 : 0 and iso-C16 : 0 were identified as the main cellular fatty acids.
30444470	9	43	dep	C16 	908:911	arg1	 0					938:939	 0	938:939	 0	938:939	10-methyl C17 : 0, C16 : 0, C17 : 0 and iso-C16 : 0 were identified as the main cellular fatty acids.
30444470	9	43	dep	C16 	908:911	arg1	iso-C16 					929:936	iso-C16 	929:936	iso-C16 	929:936	10-methyl C17 : 0, C16 : 0, C17 : 0 and iso-C16 : 0 were identified as the main cellular fatty acids.
30444470	7	44	theme	unidentified	794:805	arg1	phospholipids					807:819	three unidentified phospholipids	788:819	three unidentified phospholipids	788:819	The phospholipids comprised phosphatidylmethylethanolamine, hydroxyphosphatidylmethylethanolamine, phosphatidylethanolamine, hydroxyphosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol, four phosphoglycolipids and three unidentified phospholipids.
30444470	11	45	theme	genus	1124:1128	arg1	Thermocatellispora					1130:1147	the genus Thermocatellispora	1120:1147	the genus Thermocatellispora	1120:1147	Analysis of the 16S rRNA gene sequence showed that strain CHM3-46T belonged to the genus Thermocatellispora, exhibiting the highest similarity to Thermocatellispora tengchongensis YIM 77521T (98.5 %).
30444470	4	46	theme	short	337:341	arg1	chains					353:358	short, straight chains	337:358	short, straight chains of warty spores on aerial mycelia	337:392	Strain CHM3-46T produced short, straight chains of warty spores on aerial mycelia.
30444470	10	47	theme	DNA	1022:1024	arg1	%					1038:1038	73.2 mol%	1030:1038	73.2 mol%	1030:1038	The G+C content of the genomic DNA was 73.2 mol%.
30444470	10	47	theme	DNA	1022:1024	arg1	G+C content					995:1005	The G+C content	991:1005	The G+C content of the genomic DNA	991:1024	The G+C content of the genomic DNA was 73.2 mol%.
30444470	13	48	theme	taxonomic	1442:1450	arg1	data					1452:1455	the taxonomic data	1438:1455	the taxonomic data	1438:1455	Based on the taxonomic data, strain CHM3-46T could be readily distinguished from its closest phylogenetic relative and represents a novel species, for which the name Thermocatellisporasoli sp.
30444470	2	49	theme	aerobic	68:74	arg1	actinomycete					92:103	An aerobic, spore-forming, actinomycete	65:103	actinomycete	92:103	An aerobic, spore-forming, actinomycete, designated strain CHM3-46T, was isolated from soil in a hot spring pond located in Chiangmai province, Thailand.
30444470	3	50	theme	genus	286:290	arg1	Thermocatellispora					292:309	the genus Thermocatellispora	282:309	the genus Thermocatellispora	282:309	The strain exhibited taxonomic characteristics consistent with the genus Thermocatellispora.
30444470	2	51	theme	pond	173:176	arg1	spring					166:171	a hot spring pond	160:176	a hot spring pond located in Chiangmai province, Thailand	160:216	An aerobic, spore-forming, actinomycete, designated strain CHM3-46T, was isolated from soil in a hot spring pond located in Chiangmai province, Thailand.
30444470	2	52	theme	located	178:184	arg1	spring					166:171	a hot spring pond	160:176	a hot spring pond located in Chiangmai province, Thailand	160:216	An aerobic, spore-forming, actinomycete, designated strain CHM3-46T, was isolated from soil in a hot spring pond located in Chiangmai province, Thailand.
30444470	15	53	theme	type	1644:1647	arg1	strain					1649:1654	The type strain	1640:1654	The type strain	1640:1654	The type strain is CHM3-46T (=TBRC 7649T=NBRC 113148T).
30444470	15	53	theme	type	1644:1647	arg1	CHM3-46T					1659:1666	CHM3-46T	1659:1666	CHM3-46T (=TBRC 7649T=NBRC 113148T)	1659:1693	The type strain is CHM3-46T (=TBRC 7649T=NBRC 113148T).
30444470	5	54	theme	acid	431:434	arg1	presence					399:406	The presence	395:406	The presence of meso-diaminopimelic acid	395:434	The presence of meso-diaminopimelic acid was observed in the cell-wall peptidoglycan.
30444470	15	55	theme	=TBRC	1669:1673	arg1	113148T					1686:1692	=TBRC 7649T=NBRC 113148T	1669:1692	=TBRC 7649T=NBRC 113148T	1669:1692	The type strain is CHM3-46T (=TBRC 7649T=NBRC 113148T).
30444470	15	55	theme	=TBRC	1669:1673	arg1	CHM3-46T					1659:1666	CHM3-46T	1659:1666	CHM3-46T (=TBRC 7649T=NBRC 113148T)	1659:1693	The type strain is CHM3-46T (=TBRC 7649T=NBRC 113148T).
30444470	3	56	theme	consistent	266:275	arg1	characteristics					250:264	taxonomic characteristics	240:264	taxonomic characteristics consistent with the genus Thermocatellispora	240:309	The strain exhibited taxonomic characteristics consistent with the genus Thermocatellispora.
30444470	12	57	theme	several	1300:1306	arg1	differences					1353:1363	several physiological and biochemical characteristic differences	1300:1363	several physiological and biochemical characteristic differences	1300:1363	Furthermore, a low DNA relatedness value (23.4 %±0.8) and several physiological and biochemical characteristic differences were detected between strain CHM3-46T and its closest relative.
30444470	10	58	theme	genomic	1014:1020	arg1	DNA					1022:1024	the genomic DNA	1010:1024	the genomic DNA	1010:1024	The G+C content of the genomic DNA was 73.2 mol%.
30444470	12	59	theme	biochemical	1326:1336	arg1	differences					1353:1363	several physiological and biochemical characteristic differences	1300:1363	several physiological and biochemical characteristic differences	1300:1363	Furthermore, a low DNA relatedness value (23.4 %±0.8) and several physiological and biochemical characteristic differences were detected between strain CHM3-46T and its closest relative.
30444470	12	60	theme	low	1257:1259	arg1	value					1277:1281	a low DNA relatedness value	1255:1281	a low DNA relatedness value (23.4 %±0.8)	1255:1294	Furthermore, a low DNA relatedness value (23.4 %±0.8) and several physiological and biochemical characteristic differences were detected between strain CHM3-46T and its closest relative.
30444470	12	60	theme	low	1257:1259	arg1	%					1289:1289	23.4 %±0.8	1284:1293	23.4 %±0.8	1284:1293	Furthermore, a low DNA relatedness value (23.4 %±0.8) and several physiological and biochemical characteristic differences were detected between strain CHM3-46T and its closest relative.
30444470	12	61	theme	strain	1387:1392	arg1	CHM3-46T					1394:1401	strain CHM3-46T	1387:1401	strain CHM3-46T	1387:1401	Furthermore, a low DNA relatedness value (23.4 %±0.8) and several physiological and biochemical characteristic differences were detected between strain CHM3-46T and its closest relative.
30444470	12	62	theme	closest	1411:1417	arg1	relative					1419:1426	its closest relative	1407:1426	its closest relative	1407:1426	Furthermore, a low DNA relatedness value (23.4 %±0.8) and several physiological and biochemical characteristic differences were detected between strain CHM3-46T and its closest relative.
30444470	2	63	theme	hot	162:164	arg1	spring					166:171	a hot spring pond	160:176	a hot spring pond located in Chiangmai province, Thailand	160:216	An aerobic, spore-forming, actinomycete, designated strain CHM3-46T, was isolated from soil in a hot spring pond located in Chiangmai province, Thailand.
30444470	13	64	theme	Thermocatellisporasoli	1595:1616	arg1	sp					1618:1619	the name Thermocatellisporasoli sp	1586:1619	the name Thermocatellisporasoli sp	1586:1619	Based on the taxonomic data, strain CHM3-46T could be readily distinguished from its closest phylogenetic relative and represents a novel species, for which the name Thermocatellisporasoli sp.
30444470	13	65	theme	strain	1458:1463	arg1	CHM3-46T					1465:1472	strain CHM3-46T	1458:1472	strain CHM3-46T	1458:1472	Based on the taxonomic data, strain CHM3-46T could be readily distinguished from its closest phylogenetic relative and represents a novel species, for which the name Thermocatellisporasoli sp.
30444470	12	66	theme	relatedness	1265:1275	arg1	value					1277:1281	a low DNA relatedness value	1255:1281	a low DNA relatedness value (23.4 %±0.8)	1255:1294	Furthermore, a low DNA relatedness value (23.4 %±0.8) and several physiological and biochemical characteristic differences were detected between strain CHM3-46T and its closest relative.
30444470	12	66	theme	relatedness	1265:1275	arg1	%					1289:1289	23.4 %±0.8	1284:1293	23.4 %±0.8	1284:1293	Furthermore, a low DNA relatedness value (23.4 %±0.8) and several physiological and biochemical characteristic differences were detected between strain CHM3-46T and its closest relative.
30444470	2	67	dep	aerobic	68:74	arg1	spore-forming					77:89	spore-forming	77:89	spore-forming	77:89	An aerobic, spore-forming, actinomycete, designated strain CHM3-46T, was isolated from soil in a hot spring pond located in Chiangmai province, Thailand.
30444470	12	68	theme	physiological	1308:1320	arg1	differences					1353:1363	several physiological and biochemical characteristic differences	1300:1363	several physiological and biochemical characteristic differences	1300:1363	Furthermore, a low DNA relatedness value (23.4 %±0.8) and several physiological and biochemical characteristic differences were detected between strain CHM3-46T and its closest relative.
30444470	9	69	theme	fatty	978:982	arg1	acids					984:988	the main cellular fatty acids	960:988	the main cellular fatty acids	960:988	10-methyl C17 : 0, C16 : 0, C17 : 0 and iso-C16 : 0 were identified as the main cellular fatty acids.
30444470	9	69	theme	fatty	978:982	arg1	C16 					908:911	C16 	908:911	C16 	908:911	10-methyl C17 : 0, C16 : 0, C17 : 0 and iso-C16 : 0 were identified as the main cellular fatty acids.
30141771	9	0	theme	kwangyangense	1063:1075	arg1	21549T					1081:1086	A. kwangyangense DSM 21549T	1060:1086	A. kwangyangense DSM 21549T (36.45±0.42 %)	1060:1101	The DNA G+C content was 69.2 mol% and strain D6T showed low DNA-DNA relatedness to A. kwangyangense DSM 21549T (36.45±0.42 %).
30141771	9	0	theme	kwangyangense	1063:1075	arg1	%					1100:1100	36.45±0.42 %	1089:1100	36.45±0.42 %	1089:1100	The DNA G+C content was 69.2 mol% and strain D6T showed low DNA-DNA relatedness to A. kwangyangense DSM 21549T (36.45±0.42 %).
30141771	8	1	theme	major	915:919	arg1	lipids					927:932	The major polar lipids	911:932	The major polar lipids	911:932	The major polar lipids were a lipid, glycolipid and phospholipid.
30141771	8	1	theme	major	915:919	arg1	lipid					941:945	a lipid	939:945	a lipid	939:945	The major polar lipids were a lipid, glycolipid and phospholipid.
30141771	10	2	theme	novel	1174:1178	arg1	species					1180:1186	a novel species	1172:1186	a novel species	1172:1186	Based on these genotypic and phenotypic data, strain D6T represents a novel species in the genus Aestuariimicrobium, for which the name Aestuariimicrobium soli sp.
30141771	4	3	theme	16S	470:472	arg1	sequences					484:492	16S rRNA gene sequences	470:492	16S rRNA gene sequences	470:492	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain D6T was most closely related to Aestuariimicrobium kwangyangense DSM 21549T (98.47 %), followed by Tessaracoccus rhinocerotis YIM 101269T (94.46 %).
30141771	9	4	theme	DSM	1077:1079	arg1	21549T					1081:1086	A. kwangyangense DSM 21549T	1060:1086	A. kwangyangense DSM 21549T (36.45±0.42 %)	1060:1101	The DNA G+C content was 69.2 mol% and strain D6T showed low DNA-DNA relatedness to A. kwangyangense DSM 21549T (36.45±0.42 %).
30141771	9	4	theme	DSM	1077:1079	arg1	%					1100:1100	36.45±0.42 %	1089:1100	36.45±0.42 %	1089:1100	The DNA G+C content was 69.2 mol% and strain D6T showed low DNA-DNA relatedness to A. kwangyangense DSM 21549T (36.45±0.42 %).
30141771	5	5	contain	had	673:675	arg2	based					703:707	a cell-wall peptidoglycan based	677:707	a cell-wall peptidoglycan based	677:707	Strain D6T had a cell-wall peptidoglycan based on ll-diaminopimelic acid.
30141771	5	5	contain	had	673:675	arg1	D6T					669:671	Strain D6T	662:671	Strain D6T	662:671	Strain D6T had a cell-wall peptidoglycan based on ll-diaminopimelic acid.
30141771	10	6	theme	soli	1259:1262	arg1	sp					1264:1265	the name Aestuariimicrobium soli sp	1231:1265	the name Aestuariimicrobium soli sp	1231:1265	Based on these genotypic and phenotypic data, strain D6T represents a novel species in the genus Aestuariimicrobium, for which the name Aestuariimicrobium soli sp.
30141771	9	7	theme	strain	1015:1020	arg1	D6T					1022:1024	strain D6T	1015:1024	strain D6T	1015:1024	The DNA G+C content was 69.2 mol% and strain D6T showed low DNA-DNA relatedness to A. kwangyangense DSM 21549T (36.45±0.42 %).
30141771	10	8	theme	genus	1195:1199	arg1	Aestuariimicrobium					1201:1218	the genus Aestuariimicrobium	1191:1218	the genus Aestuariimicrobium	1191:1218	Based on these genotypic and phenotypic data, strain D6T represents a novel species in the genus Aestuariimicrobium, for which the name Aestuariimicrobium soli sp.
30141771	9	9	theme	DNA	981:983	arg1	content					989:995	The DNA G+C content	977:995	The DNA G+C content	977:995	The DNA G+C content was 69.2 mol% and strain D6T showed low DNA-DNA relatedness to A. kwangyangense DSM 21549T (36.45±0.42 %).
30141771	9	9	theme	DNA	981:983	arg1	%					1009:1009	69.2 mol%	1001:1009	69.2 mol%	1001:1009	The DNA G+C content was 69.2 mol% and strain D6T showed low DNA-DNA relatedness to A. kwangyangense DSM 21549T (36.45±0.42 %).
30141771	1	10	theme	farmland	48:55	arg1	soil					57:60	farmland soil	48:60	farmland soil	48:60	nov., isolated from farmland soil, and emended description of the genus Aestuariimicrobium.
30141771	10	11	theme	genotypic	1119:1127	arg1	data					1144:1147	these genotypic and phenotypic data	1113:1147	these genotypic and phenotypic data	1113:1147	Based on these genotypic and phenotypic data, strain D6T represents a novel species in the genus Aestuariimicrobium, for which the name Aestuariimicrobium soli sp.
30141771	4	12	theme	gene	479:482	arg1	sequences					484:492	16S rRNA gene sequences	470:492	16S rRNA gene sequences	470:492	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain D6T was most closely related to Aestuariimicrobium kwangyangense DSM 21549T (98.47 %), followed by Tessaracoccus rhinocerotis YIM 101269T (94.46 %).
30141771	6	13	theme	predominant	753:763	arg1	MK-9					736:739	MK-9	736:739	MK-9(H4)	736:743	MK-9(H4) was the predominant menaquinone.
30141771	6	13	theme	predominant	753:763	arg1	menaquinone					765:775	the predominant menaquinone	749:775	the predominant menaquinone	749:775	MK-9(H4) was the predominant menaquinone.
30141771	7	14	theme	D6T	810:812	arg1	 0					832:833	 0	832:833	 0	832:833	The major fatty acids of strain D6T were anteiso-C15 : 0, iso-C15 : 0 and summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B).
30141771	7	14	theme	D6T	810:812	arg1	anteiso-C15 					819:830	anteiso-C15 	819:830	anteiso-C15 	819:830	The major fatty acids of strain D6T were anteiso-C15 : 0, iso-C15 : 0 and summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B).
30141771	7	14	theme	D6T	810:812	arg1	acids					794:798	The major fatty acids	778:798	The major fatty acids of strain D6T	778:812	The major fatty acids of strain D6T were anteiso-C15 : 0, iso-C15 : 0 and summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B).
30141771	7	15	dep	 0	845:846	arg1	iso-C17 					870:877	iso-C17 	870:877	iso-C17 : 1 I and/or anteiso-C17 : 1 B	870:907	The major fatty acids of strain D6T were anteiso-C15 : 0, iso-C15 : 0 and summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B).
30141771	1	16	attach	isolated	34:41	arg2	nov.					28:31	nov.	28:31	nov.	28:31	nov., isolated from farmland soil, and emended description of the genus Aestuariimicrobium.
30141771	1	16	attach	isolated	34:41	arg1	soil					57:60	farmland soil	48:60	farmland soil	48:60	nov., isolated from farmland soil, and emended description of the genus Aestuariimicrobium.
30141771	7	17	dep	iso-C17 	870:877	arg1	B					907:907	 1 B	904:907	iso-C17 : 1 I and/or anteiso-C17 : 1 B	870:907	The major fatty acids of strain D6T were anteiso-C15 : 0, iso-C15 : 0 and summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B).
30141771	7	17	dep	iso-C17 	870:877	arg1	I					882:882	 1 I	879:882	 1 I	879:882	The major fatty acids of strain D6T were anteiso-C15 : 0, iso-C15 : 0 and summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B).
30141771	7	17	dep	iso-C17 	870:877	arg1	anteiso-C17 					891:902	anteiso-C17 	891:902	anteiso-C17 	891:902	The major fatty acids of strain D6T were anteiso-C15 : 0, iso-C15 : 0 and summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B).
30141771	1	18	theme	genus	94:98	arg1	Aestuariimicrobium					100:117	the genus Aestuariimicrobium	90:117	the genus Aestuariimicrobium	90:117	nov., isolated from farmland soil, and emended description of the genus Aestuariimicrobium.
30141771	10	19	theme	name	1235:1238	arg1	sp					1264:1265	the name Aestuariimicrobium soli sp	1231:1265	the name Aestuariimicrobium soli sp	1231:1265	Based on these genotypic and phenotypic data, strain D6T represents a novel species in the genus Aestuariimicrobium, for which the name Aestuariimicrobium soli sp.
30141771	9	20	theme	G+C	985:987	arg1	content					989:995	The DNA G+C content	977:995	The DNA G+C content	977:995	The DNA G+C content was 69.2 mol% and strain D6T showed low DNA-DNA relatedness to A. kwangyangense DSM 21549T (36.45±0.42 %).
30141771	9	20	theme	G+C	985:987	arg1	%					1009:1009	69.2 mol%	1001:1009	69.2 mol%	1001:1009	The DNA G+C content was 69.2 mol% and strain D6T showed low DNA-DNA relatedness to A. kwangyangense DSM 21549T (36.45±0.42 %).
30141771	4	21	theme	rRNA	474:477	arg1	sequences					484:492	16S rRNA gene sequences	470:492	16S rRNA gene sequences	470:492	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain D6T was most closely related to Aestuariimicrobium kwangyangense DSM 21549T (98.47 %), followed by Tessaracoccus rhinocerotis YIM 101269T (94.46 %).
30141771	12	22	theme	type	1290:1293	arg1	strain					1295:1300	The type strain	1286:1300	The type strain	1286:1300	The type strain is D6T (=KCTC 39995T=DSM 105824T).
30141771	12	22	theme	type	1290:1293	arg1	D6T					1305:1307	D6T	1305:1307	D6T (=KCTC 39995T=DSM 105824T)	1305:1334	The type strain is D6T (=KCTC 39995T=DSM 105824T).
30141771	3	23	theme	%	430:430	arg1	NaCl					432:435	optimum, 0.5 % NaCl	417:435	NaCl	432:435	Growth occurred at 4-37 °C (optimum, 30 °C), at pH 6.5-8.5 (optimum, 7.0) and with 0-7 % (w/v) NaCl (optimum, 0.5 % NaCl).
30141771	3	23	theme	%	430:430	arg1	NaCl					411:414	0-7 % (w/v) NaCl	399:414	0-7 % (w/v) NaCl (optimum, 0.5 % NaCl)	399:436	Growth occurred at 4-37 °C (optimum, 30 °C), at pH 6.5-8.5 (optimum, 7.0) and with 0-7 % (w/v) NaCl (optimum, 0.5 % NaCl).
30141771	0	24	theme	soli	19:22	arg1	sp					24:25	Aestuariimicrobium soli sp	0:25	Aestuariimicrobium soli sp.	0:26	Aestuariimicrobium soli sp.
30141771	2	25	theme	Gram-reaction-positive	122:143	arg1	strain					223:228	A Gram-reaction-positive, catalase-positive, non-spore-forming and short rod- or oval-shaped bacterial strain	120:228	A Gram-reaction-positive, catalase-positive, non-spore-forming and short rod- or oval-shaped bacterial strain	120:228	A Gram-reaction-positive, catalase-positive, non-spore-forming and short rod- or oval-shaped bacterial strain, designated D6T, was isolated from farmland soil in Xuancheng, Anhui Province, China.
30141771	7	26	theme	strain	803:808	arg1	D6T					810:812	strain D6T	803:812	strain D6T	803:812	The major fatty acids of strain D6T were anteiso-C15 : 0, iso-C15 : 0 and summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B).
30141771	4	27	theme	Tessaracoccus	612:624	arg1	101269T					643:649	Tessaracoccus rhinocerotis YIM 101269T	612:649	Tessaracoccus rhinocerotis YIM 101269T (94.46 %)	612:659	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain D6T was most closely related to Aestuariimicrobium kwangyangense DSM 21549T (98.47 %), followed by Tessaracoccus rhinocerotis YIM 101269T (94.46 %).
30141771	4	27	theme	Tessaracoccus	612:624	arg1	%					658:658	94.46 %	652:658	94.46 %	652:658	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain D6T was most closely related to Aestuariimicrobium kwangyangense DSM 21549T (98.47 %), followed by Tessaracoccus rhinocerotis YIM 101269T (94.46 %).
30141771	2	28	theme	catalase-positive	146:162	arg1	strain					223:228	A Gram-reaction-positive, catalase-positive, non-spore-forming and short rod- or oval-shaped bacterial strain	120:228	A Gram-reaction-positive, catalase-positive, non-spore-forming and short rod- or oval-shaped bacterial strain	120:228	A Gram-reaction-positive, catalase-positive, non-spore-forming and short rod- or oval-shaped bacterial strain, designated D6T, was isolated from farmland soil in Xuancheng, Anhui Province, China.
30141771	4	29	theme	kwangyangense	564:576	arg1	DSM					578:580	Aestuariimicrobium kwangyangense DSM 21549T	545:587	Aestuariimicrobium kwangyangense DSM 21549T (98.47 %)	545:597	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain D6T was most closely related to Aestuariimicrobium kwangyangense DSM 21549T (98.47 %), followed by Tessaracoccus rhinocerotis YIM 101269T (94.46 %).
30141771	4	29	theme	kwangyangense	564:576	arg1	%					596:596	98.47 %	590:596	98.47 %	590:596	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain D6T was most closely related to Aestuariimicrobium kwangyangense DSM 21549T (98.47 %), followed by Tessaracoccus rhinocerotis YIM 101269T (94.46 %).
30141771	0	30	theme	Aestuariimicrobium	0:17	arg1	sp					24:25	Aestuariimicrobium soli sp	0:25	Aestuariimicrobium soli sp.	0:26	Aestuariimicrobium soli sp.
30141771	4	31	theme	Phylogenetic	439:450	arg1	analysis					452:459	Phylogenetic analysis	439:459	Phylogenetic analysis based on 16S rRNA gene sequences	439:492	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain D6T was most closely related to Aestuariimicrobium kwangyangense DSM 21549T (98.47 %), followed by Tessaracoccus rhinocerotis YIM 101269T (94.46 %).
30141771	7	32	theme	 1	904:905	arg1	B					907:907	 1 B	904:907	iso-C17 : 1 I and/or anteiso-C17 : 1 B	870:907	The major fatty acids of strain D6T were anteiso-C15 : 0, iso-C15 : 0 and summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B).
30141771	7	33	theme	fatty	788:792	arg1	 0					832:833	 0	832:833	 0	832:833	The major fatty acids of strain D6T were anteiso-C15 : 0, iso-C15 : 0 and summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B).
30141771	7	33	theme	fatty	788:792	arg1	anteiso-C15 					819:830	anteiso-C15 	819:830	anteiso-C15 	819:830	The major fatty acids of strain D6T were anteiso-C15 : 0, iso-C15 : 0 and summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B).
30141771	7	33	theme	fatty	788:792	arg1	acids					794:798	The major fatty acids	778:798	The major fatty acids of strain D6T	778:812	The major fatty acids of strain D6T were anteiso-C15 : 0, iso-C15 : 0 and summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B).
30141771	10	34	theme	strain	1150:1155	arg1	D6T					1157:1159	strain D6T	1150:1159	strain D6T	1150:1159	Based on these genotypic and phenotypic data, strain D6T represents a novel species in the genus Aestuariimicrobium, for which the name Aestuariimicrobium soli sp.
30141771	7	35	dep	 0	832:833	arg1	 0					845:846	 0	845:846	 0	845:846	The major fatty acids of strain D6T were anteiso-C15 : 0, iso-C15 : 0 and summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B).
30141771	7	35	dep	 0	832:833	arg1	feature					859:865	summed feature 4	852:867	summed feature 4	852:867	The major fatty acids of strain D6T were anteiso-C15 : 0, iso-C15 : 0 and summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B).
30141771	4	36	theme	Aestuariimicrobium	545:562	arg1	DSM					578:580	Aestuariimicrobium kwangyangense DSM 21549T	545:587	Aestuariimicrobium kwangyangense DSM 21549T (98.47 %)	545:597	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain D6T was most closely related to Aestuariimicrobium kwangyangense DSM 21549T (98.47 %), followed by Tessaracoccus rhinocerotis YIM 101269T (94.46 %).
30141771	4	36	theme	Aestuariimicrobium	545:562	arg1	%					596:596	98.47 %	590:596	98.47 %	590:596	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain D6T was most closely related to Aestuariimicrobium kwangyangense DSM 21549T (98.47 %), followed by Tessaracoccus rhinocerotis YIM 101269T (94.46 %).
30141771	5	37	theme	cell-wall	679:687	arg1	based					703:707	a cell-wall peptidoglycan based	677:707	a cell-wall peptidoglycan based	677:707	Strain D6T had a cell-wall peptidoglycan based on ll-diaminopimelic acid.
30141771	9	38	theme	69.2 mol	1001:1008	arg1	content					989:995	The DNA G+C content	977:995	The DNA G+C content	977:995	The DNA G+C content was 69.2 mol% and strain D6T showed low DNA-DNA relatedness to A. kwangyangense DSM 21549T (36.45±0.42 %).
30141771	9	38	theme	69.2 mol	1001:1008	arg1	%					1009:1009	69.2 mol%	1001:1009	69.2 mol%	1001:1009	The DNA G+C content was 69.2 mol% and strain D6T showed low DNA-DNA relatedness to A. kwangyangense DSM 21549T (36.45±0.42 %).
30141771	4	39	theme	YIM	639:641	arg1	101269T					643:649	Tessaracoccus rhinocerotis YIM 101269T	612:649	Tessaracoccus rhinocerotis YIM 101269T (94.46 %)	612:659	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain D6T was most closely related to Aestuariimicrobium kwangyangense DSM 21549T (98.47 %), followed by Tessaracoccus rhinocerotis YIM 101269T (94.46 %).
30141771	4	39	theme	YIM	639:641	arg1	%					658:658	94.46 %	652:658	94.46 %	652:658	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain D6T was most closely related to Aestuariimicrobium kwangyangense DSM 21549T (98.47 %), followed by Tessaracoccus rhinocerotis YIM 101269T (94.46 %).
30141771	5	40	theme	peptidoglycan	689:701	arg1	based					703:707	a cell-wall peptidoglycan based	677:707	a cell-wall peptidoglycan based	677:707	Strain D6T had a cell-wall peptidoglycan based on ll-diaminopimelic acid.
30141771	2	41	theme	rod-	193:196	arg1	strain					223:228	A Gram-reaction-positive, catalase-positive, non-spore-forming and short rod- or oval-shaped bacterial strain	120:228	A Gram-reaction-positive, catalase-positive, non-spore-forming and short rod- or oval-shaped bacterial strain	120:228	A Gram-reaction-positive, catalase-positive, non-spore-forming and short rod- or oval-shaped bacterial strain, designated D6T, was isolated from farmland soil in Xuancheng, Anhui Province, China.
30141771	4	42	theme	rhinocerotis	626:637	arg1	101269T					643:649	Tessaracoccus rhinocerotis YIM 101269T	612:649	Tessaracoccus rhinocerotis YIM 101269T (94.46 %)	612:659	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain D6T was most closely related to Aestuariimicrobium kwangyangense DSM 21549T (98.47 %), followed by Tessaracoccus rhinocerotis YIM 101269T (94.46 %).
30141771	4	42	theme	rhinocerotis	626:637	arg1	%					658:658	94.46 %	652:658	94.46 %	652:658	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain D6T was most closely related to Aestuariimicrobium kwangyangense DSM 21549T (98.47 %), followed by Tessaracoccus rhinocerotis YIM 101269T (94.46 %).
30141771	3	43	theme	pH	364:365	arg1	optimum					376:382	optimum, 7.0	376:387	optimum	376:382	Growth occurred at 4-37 °C (optimum, 30 °C), at pH 6.5-8.5 (optimum, 7.0) and with 0-7 % (w/v) NaCl (optimum, 0.5 % NaCl).
30141771	3	43	theme	pH	364:365	arg1	6.5-8.5					367:373	pH 6.5-8.5	364:373	pH 6.5-8.5 (optimum, 7.0)	364:388	Growth occurred at 4-37 °C (optimum, 30 °C), at pH 6.5-8.5 (optimum, 7.0) and with 0-7 % (w/v) NaCl (optimum, 0.5 % NaCl).
30141771	7	44	theme	 1	879:880	arg1	I					882:882	 1 I	879:882	 1 I	879:882	The major fatty acids of strain D6T were anteiso-C15 : 0, iso-C15 : 0 and summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B).
30141771	2	45	theme	short	187:191	arg1	strain					223:228	A Gram-reaction-positive, catalase-positive, non-spore-forming and short rod- or oval-shaped bacterial strain	120:228	A Gram-reaction-positive, catalase-positive, non-spore-forming and short rod- or oval-shaped bacterial strain	120:228	A Gram-reaction-positive, catalase-positive, non-spore-forming and short rod- or oval-shaped bacterial strain, designated D6T, was isolated from farmland soil in Xuancheng, Anhui Province, China.
30141771	5	46	theme	ll-diaminopimelic	712:728	arg1	acid					730:733	ll-diaminopimelic acid	712:733	ll-diaminopimelic acid	712:733	Strain D6T had a cell-wall peptidoglycan based on ll-diaminopimelic acid.
30141771	10	47	theme	Aestuariimicrobium	1240:1257	arg1	sp					1264:1265	the name Aestuariimicrobium soli sp	1231:1265	the name Aestuariimicrobium soli sp	1231:1265	Based on these genotypic and phenotypic data, strain D6T represents a novel species in the genus Aestuariimicrobium, for which the name Aestuariimicrobium soli sp.
30141771	12	48	theme	=KCTC	1310:1314	arg1	105824T					1327:1333	=KCTC 39995T=DSM 105824T	1310:1333	=KCTC 39995T=DSM 105824T	1310:1333	The type strain is D6T (=KCTC 39995T=DSM 105824T).
30141771	12	48	theme	=KCTC	1310:1314	arg1	D6T					1305:1307	D6T	1305:1307	D6T (=KCTC 39995T=DSM 105824T)	1305:1334	The type strain is D6T (=KCTC 39995T=DSM 105824T).
30141771	7	49	theme	summed	852:857	arg1	feature					859:865	summed feature 4	852:867	summed feature 4	852:867	The major fatty acids of strain D6T were anteiso-C15 : 0, iso-C15 : 0 and summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B).
30141771	5	50	theme	Strain	662:667	arg1	D6T					669:671	Strain D6T	662:671	Strain D6T	662:671	Strain D6T had a cell-wall peptidoglycan based on ll-diaminopimelic acid.
30141771	13	51	theme	genus	1367:1371	arg1	Aestuariimicrobium					1373:1390	the genus Aestuariimicrobium	1363:1390	the genus Aestuariimicrobium	1363:1390	An emended description of the genus Aestuariimicrobium is presented.
30141771	2	52	theme	non-spore-forming	165:181	arg1	strain					223:228	A Gram-reaction-positive, catalase-positive, non-spore-forming and short rod- or oval-shaped bacterial strain	120:228	A Gram-reaction-positive, catalase-positive, non-spore-forming and short rod- or oval-shaped bacterial strain	120:228	A Gram-reaction-positive, catalase-positive, non-spore-forming and short rod- or oval-shaped bacterial strain, designated D6T, was isolated from farmland soil in Xuancheng, Anhui Province, China.
30141771	3	53	theme	w/v	406:408	arg1	NaCl					432:435	optimum, 0.5 % NaCl	417:435	NaCl	432:435	Growth occurred at 4-37 °C (optimum, 30 °C), at pH 6.5-8.5 (optimum, 7.0) and with 0-7 % (w/v) NaCl (optimum, 0.5 % NaCl).
30141771	3	53	theme	w/v	406:408	arg1	NaCl					411:414	0-7 % (w/v) NaCl	399:414	0-7 % (w/v) NaCl (optimum, 0.5 % NaCl)	399:436	Growth occurred at 4-37 °C (optimum, 30 °C), at pH 6.5-8.5 (optimum, 7.0) and with 0-7 % (w/v) NaCl (optimum, 0.5 % NaCl).
30141771	13	54	theme	Aestuariimicrobium	1373:1390	arg1	description					1348:1358	An emended description	1337:1358	An emended description of the genus Aestuariimicrobium	1337:1390	An emended description of the genus Aestuariimicrobium is presented.
30141771	9	55	theme	low	1033:1035	arg1	relatedness					1045:1055	low DNA-DNA relatedness	1033:1055	low DNA-DNA relatedness to A. kwangyangense DSM 21549T (36.45±0.42 %)	1033:1101	The DNA G+C content was 69.2 mol% and strain D6T showed low DNA-DNA relatedness to A. kwangyangense DSM 21549T (36.45±0.42 %).
30141771	9	56	theme	DNA-DNA	1037:1043	arg1	relatedness					1045:1055	low DNA-DNA relatedness	1033:1055	low DNA-DNA relatedness to A. kwangyangense DSM 21549T (36.45±0.42 %)	1033:1101	The DNA G+C content was 69.2 mol% and strain D6T showed low DNA-DNA relatedness to A. kwangyangense DSM 21549T (36.45±0.42 %).
30141771	1	57	theme	Aestuariimicrobium	100:117	arg1	description					75:85	description	75:85	description of the genus Aestuariimicrobium	75:117	nov., isolated from farmland soil, and emended description of the genus Aestuariimicrobium.
30141771	8	58	theme	polar	921:925	arg1	lipids					927:932	The major polar lipids	911:932	The major polar lipids	911:932	The major polar lipids were a lipid, glycolipid and phospholipid.
30141771	8	58	theme	polar	921:925	arg1	lipid					941:945	a lipid	939:945	a lipid	939:945	The major polar lipids were a lipid, glycolipid and phospholipid.
30141771	2	59	theme	bacterial	213:221	arg1	strain					223:228	A Gram-reaction-positive, catalase-positive, non-spore-forming and short rod- or oval-shaped bacterial strain	120:228	A Gram-reaction-positive, catalase-positive, non-spore-forming and short rod- or oval-shaped bacterial strain	120:228	A Gram-reaction-positive, catalase-positive, non-spore-forming and short rod- or oval-shaped bacterial strain, designated D6T, was isolated from farmland soil in Xuancheng, Anhui Province, China.
30141771	2	60	theme	farmland	265:272	arg1	soil					274:277	farmland soil	265:277	farmland soil in Xuancheng, Anhui Province, China	265:313	A Gram-reaction-positive, catalase-positive, non-spore-forming and short rod- or oval-shaped bacterial strain, designated D6T, was isolated from farmland soil in Xuancheng, Anhui Province, China.
30141771	3	61	theme	%	403:403	arg1	NaCl					432:435	optimum, 0.5 % NaCl	417:435	NaCl	432:435	Growth occurred at 4-37 °C (optimum, 30 °C), at pH 6.5-8.5 (optimum, 7.0) and with 0-7 % (w/v) NaCl (optimum, 0.5 % NaCl).
30141771	3	61	theme	%	403:403	arg1	NaCl					411:414	0-7 % (w/v) NaCl	399:414	0-7 % (w/v) NaCl (optimum, 0.5 % NaCl)	399:436	Growth occurred at 4-37 °C (optimum, 30 °C), at pH 6.5-8.5 (optimum, 7.0) and with 0-7 % (w/v) NaCl (optimum, 0.5 % NaCl).
30141771	2	62	from	soil	274:277	arg1	Anhui					293:297	Anhui	293:297	Anhui	293:297	A Gram-reaction-positive, catalase-positive, non-spore-forming and short rod- or oval-shaped bacterial strain, designated D6T, was isolated from farmland soil in Xuancheng, Anhui Province, China.
30141771	2	62	from	soil	274:277	arg1	China					309:313	China	309:313	China	309:313	A Gram-reaction-positive, catalase-positive, non-spore-forming and short rod- or oval-shaped bacterial strain, designated D6T, was isolated from farmland soil in Xuancheng, Anhui Province, China.
30141771	3	63	dep	optimum	344:350	arg1	°C					356:357	30 °C	353:357	30 °C	353:357	Growth occurred at 4-37 °C (optimum, 30 °C), at pH 6.5-8.5 (optimum, 7.0) and with 0-7 % (w/v) NaCl (optimum, 0.5 % NaCl).
30141771	2	64	theme	oval-shaped	201:211	arg1	strain					223:228	A Gram-reaction-positive, catalase-positive, non-spore-forming and short rod- or oval-shaped bacterial strain	120:228	A Gram-reaction-positive, catalase-positive, non-spore-forming and short rod- or oval-shaped bacterial strain	120:228	A Gram-reaction-positive, catalase-positive, non-spore-forming and short rod- or oval-shaped bacterial strain, designated D6T, was isolated from farmland soil in Xuancheng, Anhui Province, China.
30141771	13	65	theme	emended	1340:1346	arg1	description					1348:1358	An emended description	1337:1358	An emended description of the genus Aestuariimicrobium	1337:1390	An emended description of the genus Aestuariimicrobium is presented.
30141771	7	66	dep	anteiso-C15 	819:830	arg1	 0					832:833	 0	832:833	 0	832:833	The major fatty acids of strain D6T were anteiso-C15 : 0, iso-C15 : 0 and summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B).
30141771	7	66	dep	anteiso-C15 	819:830	arg1	anteiso-C15 					819:830	anteiso-C15 	819:830	anteiso-C15 	819:830	The major fatty acids of strain D6T were anteiso-C15 : 0, iso-C15 : 0 and summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B).
30141771	7	66	dep	anteiso-C15 	819:830	arg1	acids					794:798	The major fatty acids	778:798	The major fatty acids of strain D6T	778:812	The major fatty acids of strain D6T were anteiso-C15 : 0, iso-C15 : 0 and summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B).
30141771	12	67	theme	39995T=DSM	1316:1325	arg1	105824T					1327:1333	=KCTC 39995T=DSM 105824T	1310:1333	=KCTC 39995T=DSM 105824T	1310:1333	The type strain is D6T (=KCTC 39995T=DSM 105824T).
30141771	12	67	theme	39995T=DSM	1316:1325	arg1	D6T					1305:1307	D6T	1305:1307	D6T (=KCTC 39995T=DSM 105824T)	1305:1334	The type strain is D6T (=KCTC 39995T=DSM 105824T).
30141771	10	68	from	species	1180:1186	arg1	Aestuariimicrobium					1201:1218	the genus Aestuariimicrobium	1191:1218	the genus Aestuariimicrobium	1191:1218	Based on these genotypic and phenotypic data, strain D6T represents a novel species in the genus Aestuariimicrobium, for which the name Aestuariimicrobium soli sp.
30141771	10	69	theme	phenotypic	1133:1142	arg1	data					1144:1147	these genotypic and phenotypic data	1113:1147	these genotypic and phenotypic data	1113:1147	Based on these genotypic and phenotypic data, strain D6T represents a novel species in the genus Aestuariimicrobium, for which the name Aestuariimicrobium soli sp.
30141771	3	70	theme	optimum	417:423	arg1	NaCl					432:435	optimum, 0.5 % NaCl	417:435	NaCl	432:435	Growth occurred at 4-37 °C (optimum, 30 °C), at pH 6.5-8.5 (optimum, 7.0) and with 0-7 % (w/v) NaCl (optimum, 0.5 % NaCl).
30141771	3	70	theme	optimum	417:423	arg1	NaCl					411:414	0-7 % (w/v) NaCl	399:414	0-7 % (w/v) NaCl (optimum, 0.5 % NaCl)	399:436	Growth occurred at 4-37 °C (optimum, 30 °C), at pH 6.5-8.5 (optimum, 7.0) and with 0-7 % (w/v) NaCl (optimum, 0.5 % NaCl).
30141771	7	71	theme	major	782:786	arg1	 0					832:833	 0	832:833	 0	832:833	The major fatty acids of strain D6T were anteiso-C15 : 0, iso-C15 : 0 and summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B).
30141771	7	71	theme	major	782:786	arg1	anteiso-C15 					819:830	anteiso-C15 	819:830	anteiso-C15 	819:830	The major fatty acids of strain D6T were anteiso-C15 : 0, iso-C15 : 0 and summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B).
30141771	7	71	theme	major	782:786	arg1	acids					794:798	The major fatty acids	778:798	The major fatty acids of strain D6T	778:812	The major fatty acids of strain D6T were anteiso-C15 : 0, iso-C15 : 0 and summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B).
30141771	4	72	theme	strain	506:511	arg1	D6T					513:515	strain D6T	506:515	strain D6T	506:515	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain D6T was most closely related to Aestuariimicrobium kwangyangense DSM 21549T (98.47 %), followed by Tessaracoccus rhinocerotis YIM 101269T (94.46 %).
30141771	9	73	theme	A.	1060:1061	arg1	21549T					1081:1086	A. kwangyangense DSM 21549T	1060:1086	A. kwangyangense DSM 21549T (36.45±0.42 %)	1060:1101	The DNA G+C content was 69.2 mol% and strain D6T showed low DNA-DNA relatedness to A. kwangyangense DSM 21549T (36.45±0.42 %).
30141771	9	73	theme	A.	1060:1061	arg1	%					1100:1100	36.45±0.42 %	1089:1100	36.45±0.42 %	1089:1100	The DNA G+C content was 69.2 mol% and strain D6T showed low DNA-DNA relatedness to A. kwangyangense DSM 21549T (36.45±0.42 %).
30141771	4	74	dep	related	534:540	arg1	followed					600:607	followed	600:607	followed by Tessaracoccus rhinocerotis YIM 101269T (94.46 %)	600:659	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain D6T was most closely related to Aestuariimicrobium kwangyangense DSM 21549T (98.47 %), followed by Tessaracoccus rhinocerotis YIM 101269T (94.46 %).
30141771	2	75	attach	isolated	251:258	arg2	strain					223:228	A Gram-reaction-positive, catalase-positive, non-spore-forming and short rod- or oval-shaped bacterial strain	120:228	A Gram-reaction-positive, catalase-positive, non-spore-forming and short rod- or oval-shaped bacterial strain	120:228	A Gram-reaction-positive, catalase-positive, non-spore-forming and short rod- or oval-shaped bacterial strain, designated D6T, was isolated from farmland soil in Xuancheng, Anhui Province, China.
30141771	2	75	attach	isolated	251:258	arg1	soil					274:277	farmland soil	265:277	farmland soil in Xuancheng, Anhui Province, China	265:313	A Gram-reaction-positive, catalase-positive, non-spore-forming and short rod- or oval-shaped bacterial strain, designated D6T, was isolated from farmland soil in Xuancheng, Anhui Province, China.
29458473	8	0	theme	summed	1011:1016	arg1	feature					1018:1024	summed feature 3	1011:1026	summed feature 3	1011:1026	The only isoprenoid quinone was MK-9(H4), and the major fatty acids detected were C17 : 1ω8c, C16 : 0, iso-C15 : 0 and summed feature 3.
29458473	5	1	theme	cultured	601:608	arg1	members					610:616	cultured members	601:616	cultured members of the genera Modestobacter and Sporichthya	601:660	Phylogenetic analysis based on 16S rRNA gene sequences showed that the novel isolate formed a cluster with several uncultured bacterial clones and with cultured members of the genera Modestobacter and Sporichthya.
29458473	6	2	theme	above	767:771	arg1	genera					773:778	the above genera	763:778	the above genera	763:778	The 16S rRNA gene sequence similarities with respect to the type strains of recognized species from the above genera and other phylogenetic neighbours ranged from 92.6 to 93.4 %.
29458473	5	3	theme	novel	520:524	arg1	isolate					526:532	the novel isolate	516:532	the novel isolate	516:532	Phylogenetic analysis based on 16S rRNA gene sequences showed that the novel isolate formed a cluster with several uncultured bacterial clones and with cultured members of the genera Modestobacter and Sporichthya.
29458473	8	4	theme	isoprenoid	901:910	arg1	MK-9					924:927	MK-9	924:927	MK-9	924:927	The only isoprenoid quinone was MK-9(H4), and the major fatty acids detected were C17 : 1ω8c, C16 : 0, iso-C15 : 0 and summed feature 3.
29458473	8	4	theme	isoprenoid	901:910	arg1	quinone					912:918	The only isoprenoid quinone	892:918	The only isoprenoid quinone	892:918	The only isoprenoid quinone was MK-9(H4), and the major fatty acids detected were C17 : 1ω8c, C16 : 0, iso-C15 : 0 and summed feature 3.
29458473	12	5	from	species	1619:1625	arg1	Sporichthyaceae					1641:1655	the family Sporichthyaceae	1630:1655	the family Sporichthyaceae	1630:1655	The combination of the genotypic and phenotypic data allowed differentiation of strain EPI-7T from its closest phylogenetic neighbours and provided evidence that strain EPI-7T represents a novel genus and species in the family Sporichthyaceae.
29458473	2	6	theme	actinobacterial	131:145	arg1	strain					147:152	A novel actinobacterial strain	123:152	A novel actinobacterial strain	123:152	A novel actinobacterial strain, designated EPI-7T, was isolated on R2A agar from human skin (keratinocytes) and subjected to a taxonomic study using a polyphasic approach.
29458473	12	7	from	genus	1609:1613	arg1	Sporichthyaceae					1641:1655	the family Sporichthyaceae	1630:1655	the family Sporichthyaceae	1630:1655	The combination of the genotypic and phenotypic data allowed differentiation of strain EPI-7T from its closest phylogenetic neighbours and provided evidence that strain EPI-7T represents a novel genus and species in the family Sporichthyaceae.
29458473	5	8	theme	bacterial	575:583	arg1	clones					585:590	several uncultured bacterial clones	556:590	several uncultured bacterial clones	556:590	Phylogenetic analysis based on 16S rRNA gene sequences showed that the novel isolate formed a cluster with several uncultured bacterial clones and with cultured members of the genera Modestobacter and Sporichthya.
29458473	6	9	theme	recognized	739:748	arg1	species					750:756	recognized species	739:756	recognized species from the above genera and other phylogenetic neighbours	739:812	The 16S rRNA gene sequence similarities with respect to the type strains of recognized species from the above genera and other phylogenetic neighbours ranged from 92.6 to 93.4 %.
29458473	5	10	theme	Phylogenetic	449:460	arg1	analysis					462:469	Phylogenetic analysis	449:469	Phylogenetic analysis based on 16S rRNA gene sequences	449:502	Phylogenetic analysis based on 16S rRNA gene sequences showed that the novel isolate formed a cluster with several uncultured bacterial clones and with cultured members of the genera Modestobacter and Sporichthya.
29458473	6	11	from	neighbours	803:812	arg1	strains					728:734	the type strains	719:734	the type strains of recognized species from the above genera and other phylogenetic neighbours	719:812	The 16S rRNA gene sequence similarities with respect to the type strains of recognized species from the above genera and other phylogenetic neighbours ranged from 92.6 to 93.4 %.
29458473	6	11	from	neighbours	803:812	arg1	species					750:756	recognized species	739:756	recognized species from the above genera and other phylogenetic neighbours	739:812	The 16S rRNA gene sequence similarities with respect to the type strains of recognized species from the above genera and other phylogenetic neighbours ranged from 92.6 to 93.4 %.
29458473	9	12	theme	major	1033:1037	arg1	lipids					1045:1050	The major polar lipids	1029:1050	The major polar lipids	1029:1050	The major polar lipids were found to be phosphatidylethanolamine, phosphatidylinositol, three unidentified phospholipids, phosphatidylglycerol, phosphatidylcholine, two unidentified amino lipids and three unidentified lipids.
29458473	9	12	theme	major	1033:1037	arg1	phosphatidylethanolamine					1069:1092	phosphatidylethanolamine	1069:1092	phosphatidylethanolamine	1069:1092	The major polar lipids were found to be phosphatidylethanolamine, phosphatidylinositol, three unidentified phospholipids, phosphatidylglycerol, phosphatidylcholine, two unidentified amino lipids and three unidentified lipids.
29458473	2	13	theme	taxonomic	250:258	arg1	study					260:264	a taxonomic study	248:264	a taxonomic study using a polyphasic approach	248:292	A novel actinobacterial strain, designated EPI-7T, was isolated on R2A agar from human skin (keratinocytes) and subjected to a taxonomic study using a polyphasic approach.
29458473	13	14	theme	gen.	1696:1699	arg1	nov.					1701:1704	The name Epidermidibacterium keratini gen. nov.	1658:1704	The name Epidermidibacterium keratini gen. nov.	1658:1704	The name Epidermidibacterium keratini gen. nov., sp.
29458473	5	15	theme	gene	489:492	arg1	sequences					494:502	16S rRNA gene sequences	480:502	16S rRNA gene sequences	480:502	Phylogenetic analysis based on 16S rRNA gene sequences showed that the novel isolate formed a cluster with several uncultured bacterial clones and with cultured members of the genera Modestobacter and Sporichthya.
29458473	5	16	theme	genera	625:630	arg1	members					610:616	cultured members	601:616	cultured members of the genera Modestobacter and Sporichthya	601:660	Phylogenetic analysis based on 16S rRNA gene sequences showed that the novel isolate formed a cluster with several uncultured bacterial clones and with cultured members of the genera Modestobacter and Sporichthya.
29458473	9	17	theme	unidentified	1123:1134	arg1	phospholipids					1136:1148	three unidentified phospholipids	1117:1148	three unidentified phospholipids	1117:1148	The major polar lipids were found to be phosphatidylethanolamine, phosphatidylinositol, three unidentified phospholipids, phosphatidylglycerol, phosphatidylcholine, two unidentified amino lipids and three unidentified lipids.
29458473	9	17	theme	unidentified	1123:1134	arg1	phosphatidylethanolamine					1069:1092	phosphatidylethanolamine	1069:1092	phosphatidylethanolamine	1069:1092	The major polar lipids were found to be phosphatidylethanolamine, phosphatidylinositol, three unidentified phospholipids, phosphatidylglycerol, phosphatidylcholine, two unidentified amino lipids and three unidentified lipids.
29458473	2	18	theme	R2A	190:192	arg1	agar					194:197	R2A agar	190:197	R2A agar from human skin (keratinocytes)	190:229	A novel actinobacterial strain, designated EPI-7T, was isolated on R2A agar from human skin (keratinocytes) and subjected to a taxonomic study using a polyphasic approach.
29458473	12	19	theme	phylogenetic	1525:1536	arg1	neighbours					1538:1547	its closest phylogenetic neighbours	1513:1547	its closest phylogenetic neighbours	1513:1547	The combination of the genotypic and phenotypic data allowed differentiation of strain EPI-7T from its closest phylogenetic neighbours and provided evidence that strain EPI-7T represents a novel genus and species in the family Sporichthyaceae.
29458473	2	20	theme	skin	210:213	arg1	keratinocytes					216:228	human skin (keratinocytes)	204:229	human skin (keratinocytes)	204:229	A novel actinobacterial strain, designated EPI-7T, was isolated on R2A agar from human skin (keratinocytes) and subjected to a taxonomic study using a polyphasic approach.
29458473	5	21	theme	16S	480:482	arg1	sequences					494:502	16S rRNA gene sequences	480:502	16S rRNA gene sequences	480:502	Phylogenetic analysis based on 16S rRNA gene sequences showed that the novel isolate formed a cluster with several uncultured bacterial clones and with cultured members of the genera Modestobacter and Sporichthya.
29458473	12	22	theme	EPI-7T	1501:1506	arg1	differentiation					1475:1489	differentiation	1475:1489	differentiation of strain EPI-7T from its closest phylogenetic neighbours	1475:1547	The combination of the genotypic and phenotypic data allowed differentiation of strain EPI-7T from its closest phylogenetic neighbours and provided evidence that strain EPI-7T represents a novel genus and species in the family Sporichthyaceae.
29458473	14	23	theme	=KCCM	1763:1767	arg1	31644T					1780:1785	=KCCM 90264T=JCM 31644T	1763:1785	=KCCM 90264T=JCM 31644T	1763:1785	nov. is proposed with the type strain being EPI-7T (=KCCM 90264T=JCM 31644T).
29458473	14	23	theme	=KCCM	1763:1767	arg1	EPI-7T					1755:1760	EPI-7T	1755:1760	EPI-7T (=KCCM 90264T=JCM 31644T)	1755:1786	nov. is proposed with the type strain being EPI-7T (=KCCM 90264T=JCM 31644T).
29458473	12	24	theme	phenotypic	1451:1460	arg1	data					1462:1465	the genotypic and phenotypic data	1433:1465	the genotypic and phenotypic data	1433:1465	The combination of the genotypic and phenotypic data allowed differentiation of strain EPI-7T from its closest phylogenetic neighbours and provided evidence that strain EPI-7T represents a novel genus and species in the family Sporichthyaceae.
29458473	13	25	theme	name	1662:1665	arg1	nov.					1701:1704	The name Epidermidibacterium keratini gen. nov.	1658:1704	The name Epidermidibacterium keratini gen. nov.	1658:1704	The name Epidermidibacterium keratini gen. nov., sp.
29458473	9	26	theme	unidentified	1198:1209	arg1	lipids					1217:1222	two unidentified amino lipids	1194:1222	two unidentified amino lipids	1194:1222	The major polar lipids were found to be phosphatidylethanolamine, phosphatidylinositol, three unidentified phospholipids, phosphatidylglycerol, phosphatidylcholine, two unidentified amino lipids and three unidentified lipids.
29458473	9	26	theme	unidentified	1198:1209	arg1	phosphatidylethanolamine					1069:1092	phosphatidylethanolamine	1069:1092	phosphatidylethanolamine	1069:1092	The major polar lipids were found to be phosphatidylethanolamine, phosphatidylinositol, three unidentified phospholipids, phosphatidylglycerol, phosphatidylcholine, two unidentified amino lipids and three unidentified lipids.
29458473	8	27	theme	major	942:946	arg1	acids					954:958	the major fatty acids	938:958	the major fatty acids detected	938:967	The only isoprenoid quinone was MK-9(H4), and the major fatty acids detected were C17 : 1ω8c, C16 : 0, iso-C15 : 0 and summed feature 3.
29458473	8	27	theme	major	942:946	arg1	C17 					974:977	C17 	974:977	C17 	974:977	The only isoprenoid quinone was MK-9(H4), and the major fatty acids detected were C17 : 1ω8c, C16 : 0, iso-C15 : 0 and summed feature 3.
29458473	0	28	theme	Epidermidibacterium	0:18	arg1	nov.					34:37	Epidermidibacterium keratini gen. nov.	0:37	Epidermidibacterium keratini gen. nov.	0:37	Epidermidibacterium keratini gen. nov., sp.
29458473	7	29	theme	genomic	865:871	arg1	DNA					873:875	the genomic DNA	861:875	the genomic DNA	861:875	The G+C content of the genomic DNA was 68.9 mol%.
29458473	1	30	theme	family	66:71	arg1	Sporichthyaceae					73:87	the family Sporichthyaceae	62:87	the family Sporichthyaceae	62:87	nov., a member of the family Sporichthyaceae, isolated from keratin epidermis.
29458473	10	31	theme	meso-diaminopimelic	1293:1311	arg1	acid					1313:1316	meso-diaminopimelic acid	1293:1316	meso-diaminopimelic acid	1293:1316	The cell-wall peptidoglycan contained meso-diaminopimelic acid, glutamic acid and alanine.
29458473	0	32	theme	gen.	29:32	arg1	nov.					34:37	Epidermidibacterium keratini gen. nov.	0:37	Epidermidibacterium keratini gen. nov.	0:37	Epidermidibacterium keratini gen. nov., sp.
29458473	10	33	theme	glutamic	1319:1326	arg1	acid					1328:1331	glutamic acid	1319:1331	glutamic acid	1319:1331	The cell-wall peptidoglycan contained meso-diaminopimelic acid, glutamic acid and alanine.
29458473	14	34	theme	type	1737:1740	arg1	EPI-7T					1755:1760	EPI-7T	1755:1760	EPI-7T (=KCCM 90264T=JCM 31644T)	1755:1786	nov. is proposed with the type strain being EPI-7T (=KCCM 90264T=JCM 31644T).
29458473	14	34	theme	type	1737:1740	arg1	strain					1742:1747	the type strain	1733:1747	the type strain	1733:1747	nov. is proposed with the type strain being EPI-7T (=KCCM 90264T=JCM 31644T).
29458473	6	35	theme	phylogenetic	790:801	arg1	neighbours					803:812	other phylogenetic neighbours	784:812	other phylogenetic neighbours	784:812	The 16S rRNA gene sequence similarities with respect to the type strains of recognized species from the above genera and other phylogenetic neighbours ranged from 92.6 to 93.4 %.
29458473	8	36	dep	C17 	974:977	arg1	 0					991:992	 0	991:992	 0	991:992	The only isoprenoid quinone was MK-9(H4), and the major fatty acids detected were C17 : 1ω8c, C16 : 0, iso-C15 : 0 and summed feature 3.
29458473	8	36	dep	C17 	974:977	arg1	C16 					986:989	C16 	986:989	C16 	986:989	The only isoprenoid quinone was MK-9(H4), and the major fatty acids detected were C17 : 1ω8c, C16 : 0, iso-C15 : 0 and summed feature 3.
29458473	6	37	theme	sequence	681:688	arg1	similarities					690:701	The 16S rRNA gene sequence similarities	663:701	The 16S rRNA gene sequence similarities with respect to the type strains of recognized species from the above genera and other phylogenetic neighbours	663:812	The 16S rRNA gene sequence similarities with respect to the type strains of recognized species from the above genera and other phylogenetic neighbours ranged from 92.6 to 93.4 %.
29458473	6	38	theme	rRNA	671:674	arg1	similarities					690:701	The 16S rRNA gene sequence similarities	663:701	The 16S rRNA gene sequence similarities with respect to the type strains of recognized species from the above genera and other phylogenetic neighbours	663:812	The 16S rRNA gene sequence similarities with respect to the type strains of recognized species from the above genera and other phylogenetic neighbours ranged from 92.6 to 93.4 %.
29458473	9	39	theme	polar	1039:1043	arg1	lipids					1045:1050	The major polar lipids	1029:1050	The major polar lipids	1029:1050	The major polar lipids were found to be phosphatidylethanolamine, phosphatidylinositol, three unidentified phospholipids, phosphatidylglycerol, phosphatidylcholine, two unidentified amino lipids and three unidentified lipids.
29458473	9	39	theme	polar	1039:1043	arg1	phosphatidylethanolamine					1069:1092	phosphatidylethanolamine	1069:1092	phosphatidylethanolamine	1069:1092	The major polar lipids were found to be phosphatidylethanolamine, phosphatidylinositol, three unidentified phospholipids, phosphatidylglycerol, phosphatidylcholine, two unidentified amino lipids and three unidentified lipids.
29458473	2	40	theme	novel	125:129	arg1	strain					147:152	A novel actinobacterial strain	123:152	A novel actinobacterial strain	123:152	A novel actinobacterial strain, designated EPI-7T, was isolated on R2A agar from human skin (keratinocytes) and subjected to a taxonomic study using a polyphasic approach.
29458473	5	41	theme	uncultured	564:573	arg1	clones					585:590	several uncultured bacterial clones	556:590	several uncultured bacterial clones	556:590	Phylogenetic analysis based on 16S rRNA gene sequences showed that the novel isolate formed a cluster with several uncultured bacterial clones and with cultured members of the genera Modestobacter and Sporichthya.
29458473	6	42	theme	species	750:756	arg1	strains					728:734	the type strains	719:734	the type strains of recognized species from the above genera and other phylogenetic neighbours	719:812	The 16S rRNA gene sequence similarities with respect to the type strains of recognized species from the above genera and other phylogenetic neighbours ranged from 92.6 to 93.4 %.
29458473	6	43	theme	type	723:726	arg1	strains					728:734	the type strains	719:734	the type strains of recognized species from the above genera and other phylogenetic neighbours	719:812	The 16S rRNA gene sequence similarities with respect to the type strains of recognized species from the above genera and other phylogenetic neighbours ranged from 92.6 to 93.4 %.
29458473	13	44	theme	keratini	1687:1694	arg1	nov.					1701:1704	The name Epidermidibacterium keratini gen. nov.	1658:1704	The name Epidermidibacterium keratini gen. nov.	1658:1704	The name Epidermidibacterium keratini gen. nov., sp.
29458473	2	45	theme	human	204:208	arg1	keratinocytes					216:228	human skin (keratinocytes)	204:229	human skin (keratinocytes)	204:229	A novel actinobacterial strain, designated EPI-7T, was isolated on R2A agar from human skin (keratinocytes) and subjected to a taxonomic study using a polyphasic approach.
29458473	12	46	theme	closest	1517:1523	arg1	neighbours					1538:1547	its closest phylogenetic neighbours	1513:1547	its closest phylogenetic neighbours	1513:1547	The combination of the genotypic and phenotypic data allowed differentiation of strain EPI-7T from its closest phylogenetic neighbours and provided evidence that strain EPI-7T represents a novel genus and species in the family Sporichthyaceae.
29458473	3	47	contain	had	387:389	arg1	cells					381:385	cells	381:385	cells	381:385	Strain EPI-7T showed a Gram-positive reaction, was non-motile, non-spore-forming, and cells had a rod-shape.
29458473	3	47	contain	had	387:389	arg2	rod-shape					393:401	rod-shape	393:401	rod-shape	393:401	Strain EPI-7T showed a Gram-positive reaction, was non-motile, non-spore-forming, and cells had a rod-shape.
29458473	11	48	dep	present	1364:1370	arg1	included					1372:1379	included	1372:1379	present included rhamnose, glucose and galactose	1364:1411	Whole-cell sugars present included rhamnose, glucose and galactose.
29458473	13	49	dep	sp	1707:1708	arg1	nov.					1701:1704	The name Epidermidibacterium keratini gen. nov.	1658:1704	The name Epidermidibacterium keratini gen. nov.	1658:1704	The name Epidermidibacterium keratini gen. nov., sp.
29458473	1	50	theme	keratin	104:110	arg1	epidermis					112:120	keratin epidermis	104:120	keratin epidermis	104:120	nov., a member of the family Sporichthyaceae, isolated from keratin epidermis.
29458473	12	51	theme	strain	1494:1499	arg1	EPI-7T					1501:1506	strain EPI-7T	1494:1506	strain EPI-7T	1494:1506	The combination of the genotypic and phenotypic data allowed differentiation of strain EPI-7T from its closest phylogenetic neighbours and provided evidence that strain EPI-7T represents a novel genus and species in the family Sporichthyaceae.
29458473	5	52	theme	rRNA	484:487	arg1	sequences					494:502	16S rRNA gene sequences	480:502	16S rRNA gene sequences	480:502	Phylogenetic analysis based on 16S rRNA gene sequences showed that the novel isolate formed a cluster with several uncultured bacterial clones and with cultured members of the genera Modestobacter and Sporichthya.
29458473	14	53	theme	90264T=JCM	1769:1778	arg1	31644T					1780:1785	=KCCM 90264T=JCM 31644T	1763:1785	=KCCM 90264T=JCM 31644T	1763:1785	nov. is proposed with the type strain being EPI-7T (=KCCM 90264T=JCM 31644T).
29458473	14	53	theme	90264T=JCM	1769:1778	arg1	EPI-7T					1755:1760	EPI-7T	1755:1760	EPI-7T (=KCCM 90264T=JCM 31644T)	1755:1786	nov. is proposed with the type strain being EPI-7T (=KCCM 90264T=JCM 31644T).
29458473	12	54	theme	data	1462:1465	arg1	combination					1418:1428	The combination	1414:1428	The combination of the genotypic and phenotypic data	1414:1465	The combination of the genotypic and phenotypic data allowed differentiation of strain EPI-7T from its closest phylogenetic neighbours and provided evidence that strain EPI-7T represents a novel genus and species in the family Sporichthyaceae.
29458473	13	55	theme	Epidermidibacterium	1667:1685	arg1	nov.					1701:1704	The name Epidermidibacterium keratini gen. nov.	1658:1704	The name Epidermidibacterium keratini gen. nov.	1658:1704	The name Epidermidibacterium keratini gen. nov., sp.
29458473	8	56	theme	fatty	948:952	arg1	acids					954:958	the major fatty acids	938:958	the major fatty acids detected	938:967	The only isoprenoid quinone was MK-9(H4), and the major fatty acids detected were C17 : 1ω8c, C16 : 0, iso-C15 : 0 and summed feature 3.
29458473	8	56	theme	fatty	948:952	arg1	C17 					974:977	C17 	974:977	C17 	974:977	The only isoprenoid quinone was MK-9(H4), and the major fatty acids detected were C17 : 1ω8c, C16 : 0, iso-C15 : 0 and summed feature 3.
29458473	12	57	theme	family	1634:1639	arg1	Sporichthyaceae					1641:1655	the family Sporichthyaceae	1630:1655	the family Sporichthyaceae	1630:1655	The combination of the genotypic and phenotypic data allowed differentiation of strain EPI-7T from its closest phylogenetic neighbours and provided evidence that strain EPI-7T represents a novel genus and species in the family Sporichthyaceae.
29458473	3	58	theme	Gram-positive	318:330	arg1	reaction					332:339	a Gram-positive reaction	316:339	a Gram-positive reaction	316:339	Strain EPI-7T showed a Gram-positive reaction, was non-motile, non-spore-forming, and cells had a rod-shape.
29458473	3	59	dep	non-motile	346:355	arg1	non-spore-forming					358:374	non-spore-forming	358:374	non-spore-forming	358:374	Strain EPI-7T showed a Gram-positive reaction, was non-motile, non-spore-forming, and cells had a rod-shape.
29458473	6	60	theme	16S	667:669	arg1	similarities					690:701	The 16S rRNA gene sequence similarities	663:701	The 16S rRNA gene sequence similarities with respect to the type strains of recognized species from the above genera and other phylogenetic neighbours	663:812	The 16S rRNA gene sequence similarities with respect to the type strains of recognized species from the above genera and other phylogenetic neighbours ranged from 92.6 to 93.4 %.
29458473	9	61	theme	amino	1211:1215	arg1	lipids					1217:1222	two unidentified amino lipids	1194:1222	two unidentified amino lipids	1194:1222	The major polar lipids were found to be phosphatidylethanolamine, phosphatidylinositol, three unidentified phospholipids, phosphatidylglycerol, phosphatidylcholine, two unidentified amino lipids and three unidentified lipids.
29458473	9	61	theme	amino	1211:1215	arg1	phosphatidylethanolamine					1069:1092	phosphatidylethanolamine	1069:1092	phosphatidylethanolamine	1069:1092	The major polar lipids were found to be phosphatidylethanolamine, phosphatidylinositol, three unidentified phospholipids, phosphatidylglycerol, phosphatidylcholine, two unidentified amino lipids and three unidentified lipids.
29458473	5	62	theme	several	556:562	arg1	clones					585:590	several uncultured bacterial clones	556:590	several uncultured bacterial clones	556:590	Phylogenetic analysis based on 16S rRNA gene sequences showed that the novel isolate formed a cluster with several uncultured bacterial clones and with cultured members of the genera Modestobacter and Sporichthya.
29458473	12	63	theme	novel	1603:1607	arg1	genus					1609:1613	a novel genus	1601:1613	a novel genus	1601:1613	The combination of the genotypic and phenotypic data allowed differentiation of strain EPI-7T from its closest phylogenetic neighbours and provided evidence that strain EPI-7T represents a novel genus and species in the family Sporichthyaceae.
29458473	5	64	dep	genera	625:630	arg1	Modestobacter					632:644	Modestobacter	632:644	Modestobacter	632:644	Phylogenetic analysis based on 16S rRNA gene sequences showed that the novel isolate formed a cluster with several uncultured bacterial clones and with cultured members of the genera Modestobacter and Sporichthya.
29458473	5	64	dep	genera	625:630	arg1	Sporichthya					650:660	Sporichthya	650:660	Sporichthya	650:660	Phylogenetic analysis based on 16S rRNA gene sequences showed that the novel isolate formed a cluster with several uncultured bacterial clones and with cultured members of the genera Modestobacter and Sporichthya.
29458473	4	65	theme	pale	436:439	arg1	yellow					441:446	pale yellow	436:446	pale yellow	436:446	Colonies were round, convex and pale yellow.
29458473	11	66	theme	Whole-cell	1346:1355	arg1	sugars					1357:1362	Whole-cell sugars	1346:1362	Whole-cell sugars	1346:1362	Whole-cell sugars present included rhamnose, glucose and galactose.
29458473	0	67	theme	keratini	20:27	arg1	nov.					34:37	Epidermidibacterium keratini gen. nov.	0:37	Epidermidibacterium keratini gen. nov.	0:37	Epidermidibacterium keratini gen. nov., sp.
29458473	7	68	theme	68.9 mol	881:888	arg1	G+C content					846:856	The G+C content	842:856	The G+C content of the genomic DNA	842:875	The G+C content of the genomic DNA was 68.9 mol%.
29458473	7	68	theme	68.9 mol	881:888	arg1	%					889:889	68.9 mol%	881:889	68.9 mol%	881:889	The G+C content of the genomic DNA was 68.9 mol%.
29458473	8	69	dep	MK-9	924:927	arg1	 0					1004:1005	 0	1004:1005	 0	1004:1005	The only isoprenoid quinone was MK-9(H4), and the major fatty acids detected were C17 : 1ω8c, C16 : 0, iso-C15 : 0 and summed feature 3.
29458473	8	69	dep	MK-9	924:927	arg1	feature					1018:1024	summed feature 3	1011:1026	summed feature 3	1011:1026	The only isoprenoid quinone was MK-9(H4), and the major fatty acids detected were C17 : 1ω8c, C16 : 0, iso-C15 : 0 and summed feature 3.
29458473	9	70	theme	unidentified	1234:1245	arg1	lipids					1247:1252	three unidentified lipids	1228:1252	three unidentified lipids	1228:1252	The major polar lipids were found to be phosphatidylethanolamine, phosphatidylinositol, three unidentified phospholipids, phosphatidylglycerol, phosphatidylcholine, two unidentified amino lipids and three unidentified lipids.
29458473	9	70	theme	unidentified	1234:1245	arg1	phosphatidylethanolamine					1069:1092	phosphatidylethanolamine	1069:1092	phosphatidylethanolamine	1069:1092	The major polar lipids were found to be phosphatidylethanolamine, phosphatidylinositol, three unidentified phospholipids, phosphatidylglycerol, phosphatidylcholine, two unidentified amino lipids and three unidentified lipids.
29458473	7	71	theme	DNA	873:875	arg1	G+C content					846:856	The G+C content	842:856	The G+C content of the genomic DNA	842:875	The G+C content of the genomic DNA was 68.9 mol%.
29458473	7	71	theme	DNA	873:875	arg1	%					889:889	68.9 mol%	881:889	68.9 mol%	881:889	The G+C content of the genomic DNA was 68.9 mol%.
29458473	1	72	theme	Sporichthyaceae	73:87	arg1	member					52:57	a member	50:57	a member of the family Sporichthyaceae	50:87	nov., a member of the family Sporichthyaceae, isolated from keratin epidermis.
29458473	1	72	theme	Sporichthyaceae	73:87	arg1	nov.					44:47	nov.	44:47	nov.	44:47	nov., a member of the family Sporichthyaceae, isolated from keratin epidermis.
29458473	12	73	theme	strain	1576:1581	arg1	EPI-7T					1583:1588	strain EPI-7T	1576:1588	strain EPI-7T	1576:1588	The combination of the genotypic and phenotypic data allowed differentiation of strain EPI-7T from its closest phylogenetic neighbours and provided evidence that strain EPI-7T represents a novel genus and species in the family Sporichthyaceae.
29458473	6	74	from	strains	728:734	arg1	genera					773:778	the above genera	763:778	the above genera	763:778	The 16S rRNA gene sequence similarities with respect to the type strains of recognized species from the above genera and other phylogenetic neighbours ranged from 92.6 to 93.4 %.
29458473	6	74	from	strains	728:734	arg1	neighbours					803:812	other phylogenetic neighbours	784:812	other phylogenetic neighbours	784:812	The 16S rRNA gene sequence similarities with respect to the type strains of recognized species from the above genera and other phylogenetic neighbours ranged from 92.6 to 93.4 %.
29458473	2	75	from	keratinocytes	216:228	arg1	agar					194:197	R2A agar	190:197	R2A agar from human skin (keratinocytes)	190:229	A novel actinobacterial strain, designated EPI-7T, was isolated on R2A agar from human skin (keratinocytes) and subjected to a taxonomic study using a polyphasic approach.
29458473	6	76	from	genera	773:778	arg1	strains					728:734	the type strains	719:734	the type strains of recognized species from the above genera and other phylogenetic neighbours	719:812	The 16S rRNA gene sequence similarities with respect to the type strains of recognized species from the above genera and other phylogenetic neighbours ranged from 92.6 to 93.4 %.
29458473	6	76	from	genera	773:778	arg1	species					750:756	recognized species	739:756	recognized species from the above genera and other phylogenetic neighbours	739:812	The 16S rRNA gene sequence similarities with respect to the type strains of recognized species from the above genera and other phylogenetic neighbours ranged from 92.6 to 93.4 %.
29458473	12	77	from	neighbours	1538:1547	arg1	differentiation					1475:1489	differentiation	1475:1489	differentiation of strain EPI-7T from its closest phylogenetic neighbours	1475:1547	The combination of the genotypic and phenotypic data allowed differentiation of strain EPI-7T from its closest phylogenetic neighbours and provided evidence that strain EPI-7T represents a novel genus and species in the family Sporichthyaceae.
29458473	12	78	theme	genotypic	1437:1445	arg1	data					1462:1465	the genotypic and phenotypic data	1433:1465	the genotypic and phenotypic data	1433:1465	The combination of the genotypic and phenotypic data allowed differentiation of strain EPI-7T from its closest phylogenetic neighbours and provided evidence that strain EPI-7T represents a novel genus and species in the family Sporichthyaceae.
29458473	0	79	dep	sp	40:41	arg1	nov.					34:37	Epidermidibacterium keratini gen. nov.	0:37	Epidermidibacterium keratini gen. nov.	0:37	Epidermidibacterium keratini gen. nov., sp.
29458473	3	80	dep	showed	309:314	arg1	non-motile					346:355	non-motile	346:355	non-motile	346:355	Strain EPI-7T showed a Gram-positive reaction, was non-motile, non-spore-forming, and cells had a rod-shape.
29458473	10	81	contain	contained	1283:1291	arg2	alanine					1337:1343	alanine	1337:1343	alanine	1337:1343	The cell-wall peptidoglycan contained meso-diaminopimelic acid, glutamic acid and alanine.
29458473	10	81	contain	contained	1283:1291	arg1	peptidoglycan					1269:1281	The cell-wall peptidoglycan	1255:1281	The cell-wall peptidoglycan	1255:1281	The cell-wall peptidoglycan contained meso-diaminopimelic acid, glutamic acid and alanine.
29458473	10	81	contain	contained	1283:1291	arg2	acid					1328:1331	glutamic acid	1319:1331	glutamic acid	1319:1331	The cell-wall peptidoglycan contained meso-diaminopimelic acid, glutamic acid and alanine.
29458473	10	81	contain	contained	1283:1291	arg2	acid					1313:1316	meso-diaminopimelic acid	1293:1316	meso-diaminopimelic acid	1293:1316	The cell-wall peptidoglycan contained meso-diaminopimelic acid, glutamic acid and alanine.
29458473	6	82	theme	other	784:788	arg1	neighbours					803:812	other phylogenetic neighbours	784:812	other phylogenetic neighbours	784:812	The 16S rRNA gene sequence similarities with respect to the type strains of recognized species from the above genera and other phylogenetic neighbours ranged from 92.6 to 93.4 %.
29458473	6	83	theme	gene	676:679	arg1	similarities					690:701	The 16S rRNA gene sequence similarities	663:701	The 16S rRNA gene sequence similarities with respect to the type strains of recognized species from the above genera and other phylogenetic neighbours	663:812	The 16S rRNA gene sequence similarities with respect to the type strains of recognized species from the above genera and other phylogenetic neighbours ranged from 92.6 to 93.4 %.
29458473	6	84	dep	93.4 	834:838	arg1	to					831:832	to	831:832	to	831:832	The 16S rRNA gene sequence similarities with respect to the type strains of recognized species from the above genera and other phylogenetic neighbours ranged from 92.6 to 93.4 %.
29458473	2	85	theme	polyphasic	274:283	arg1	approach					285:292	a polyphasic approach	272:292	a polyphasic approach	272:292	A novel actinobacterial strain, designated EPI-7T, was isolated on R2A agar from human skin (keratinocytes) and subjected to a taxonomic study using a polyphasic approach.
29458473	10	86	theme	cell-wall	1259:1267	arg1	peptidoglycan					1269:1281	The cell-wall peptidoglycan	1255:1281	The cell-wall peptidoglycan	1255:1281	The cell-wall peptidoglycan contained meso-diaminopimelic acid, glutamic acid and alanine.
30822525	2	0	theme	high	440:443	arg1	contents					453:460	high protein contents	440:460	high protein contents	440:460	More recently, Paper mulberry has also been used as forage to address the shortage of feedstuff because of its digestible crude fiber and high protein contents.
30822525	9	1	theme	significant	1727:1737	arg1	insights					1739:1746	significant insights	1727:1746	significant insights into genetic basis of the usefulness of Paper mulberry in papermaking and barkcloth making, and as forage	1727:1852	Our study provides significant insights into genetic basis of the usefulness of Paper mulberry in papermaking and barkcloth making, and as forage.
30822525	9	2	from	usefulness	1774:1783	arg1	papermaking					1806:1816	papermaking	1806:1816	papermaking	1806:1816	Our study provides significant insights into genetic basis of the usefulness of Paper mulberry in papermaking and barkcloth making, and as forage.
30822525	9	2	from	usefulness	1774:1783	arg1	making					1832:1837	making	1832:1837	making	1832:1837	Our study provides significant insights into genetic basis of the usefulness of Paper mulberry in papermaking and barkcloth making, and as forage.
30822525	9	3	theme	genetic	1753:1759	arg1	basis					1761:1765	genetic basis	1753:1765	genetic basis of the usefulness of Paper mulberry in papermaking and barkcloth making	1753:1837	Our study provides significant insights into genetic basis of the usefulness of Paper mulberry in papermaking and barkcloth making, and as forage.
30822525	1	4	theme	Lun	234:236	arg1	papermaking					238:248	Cai Lun papermaking	230:248	Cai Lun papermaking	230:248	Paper mulberry (Broussonetia papyrifera) is a well-known woody tree historically used for Cai Lun papermaking, one of the four great inventions of ancient China.
30822525	1	4	theme	Lun	234:236	arg1	inventions					273:282	the four great inventions	258:282	the four great inventions of ancient China	258:299	Paper mulberry (Broussonetia papyrifera) is a well-known woody tree historically used for Cai Lun papermaking, one of the four great inventions of ancient China.
30822525	1	4	theme	Lun	234:236	arg1	one					251:253	one	251:253	one	251:253	Paper mulberry (Broussonetia papyrifera) is a well-known woody tree historically used for Cai Lun papermaking, one of the four great inventions of ancient China.
30822525	5	5	theme	Paper	1054:1058	arg1	mulberry					1060:1067	Paper mulberry	1054:1067	Paper mulberry	1054:1067	Comparative genomic analysis revealed the expansion and contraction in the flavonoid and lignin biosynthetic gene families, respectively, accounting for the enhanced flavonoid and decreased lignin biosynthesis in Paper mulberry.
30822525	9	6	from	making	1832:1837	arg1	basis					1761:1765	genetic basis	1753:1765	genetic basis of the usefulness of Paper mulberry in papermaking and barkcloth making	1753:1837	Our study provides significant insights into genetic basis of the usefulness of Paper mulberry in papermaking and barkcloth making, and as forage.
30822525	2	7	theme	Paper	317:321	arg1	mulberry					323:330	Paper mulberry	317:330	Paper mulberry	317:330	More recently, Paper mulberry has also been used as forage to address the shortage of feedstuff because of its digestible crude fiber and high protein contents.
30822525	8	8	theme	flavonoid	1646:1654	arg1	production					1656:1665	flavonoid production	1646:1665	flavonoid production	1646:1665	Collectively, these results suggest that Paper mulberry might have undergone adaptive evolution and recruited nitrogen-fixing microbes to promote growth by enhancing flavonoid production and altering lignin monomer composition.
30822525	7	9	theme	nitrogen	1461:1468	arg1	fixation					1470:1477	symbiotic nitrogen fixation	1451:1477	symbiotic nitrogen fixation	1451:1477	We also identified the root-associated microbiota of Paper mulberry and found that Pseudomonas and Rhizobia were enriched in its roots and may provide the source of nitrogen for its stems and leaves via symbiotic nitrogen fixation.
30822525	7	10	theme	Paper	1301:1305	arg1	mulberry					1307:1314	Paper mulberry	1301:1314	Paper mulberry	1301:1314	We also identified the root-associated microbiota of Paper mulberry and found that Pseudomonas and Rhizobia were enriched in its roots and may provide the source of nitrogen for its stems and leaves via symbiotic nitrogen fixation.
30822525	5	11	theme	biosynthetic	937:948	arg1	families					955:962	the flavonoid and lignin biosynthetic gene families	912:962	families	955:962	Comparative genomic analysis revealed the expansion and contraction in the flavonoid and lignin biosynthetic gene families, respectively, accounting for the enhanced flavonoid and decreased lignin biosynthesis in Paper mulberry.
30822525	0	12	dep	Its	106:108	arg1	Forage					110:115	Forage	110:115	Forage	110:115	A Chromosome-Scale Genome Assembly of Paper Mulberry (Broussonetia papyrifera) Provides New Insights into Its Forage and Papermaking Usage.
30822525	4	13	theme	mulberry	687:694	arg1	genome					696:701	The assembled Paper mulberry genome	667:701	The assembled Paper mulberry genome	667:701	The assembled Paper mulberry genome consists of 386.83 Mb, which is close to the estimated size, and 99.25% (383.93 Mb) of the assembly was assigned to 13 pseudochromosomes.
30822525	8	14	theme	recruited	1580:1588	arg1	microbes					1606:1613	recruited nitrogen-fixing microbes	1580:1613	recruited nitrogen-fixing microbes	1580:1613	Collectively, these results suggest that Paper mulberry might have undergone adaptive evolution and recruited nitrogen-fixing microbes to promote growth by enhancing flavonoid production and altering lignin monomer composition.
30822525	0	15	theme	New	88:90	arg1	Insights					92:99	New Insights	88:99	New Insights into Its Forage and Papermaking Usage	88:137	A Chromosome-Scale Genome Assembly of Paper Mulberry (Broussonetia papyrifera) Provides New Insights into Its Forage and Papermaking Usage.
30822525	7	16	theme	symbiotic	1451:1459	arg1	fixation					1470:1477	symbiotic nitrogen fixation	1451:1477	symbiotic nitrogen fixation	1451:1477	We also identified the root-associated microbiota of Paper mulberry and found that Pseudomonas and Rhizobia were enriched in its roots and may provide the source of nitrogen for its stems and leaves via symbiotic nitrogen fixation.
30822525	6	17	from	ratio	1094:1098	arg1	mulberry					1147:1154	Paper mulberry	1141:1154	Paper mulberry	1141:1154	Moreover, the increased ratio of syringyl-lignin to guaiacyl-lignin in Paper mulberry underscores its suitability for use in medicine, forage, papermaking, and barkcloth making.
30822525	4	18	theme	Paper	681:685	arg1	genome					696:701	The assembled Paper mulberry genome	667:701	The assembled Paper mulberry genome	667:701	The assembled Paper mulberry genome consists of 386.83 Mb, which is close to the estimated size, and 99.25% (383.93 Mb) of the assembly was assigned to 13 pseudochromosomes.
30822525	1	19	theme	Broussonetia	156:167	arg1	mulberry					146:153	Paper mulberry	140:153	Paper mulberry (Broussonetia papyrifera)	140:179	Paper mulberry (Broussonetia papyrifera) is a well-known woody tree historically used for Cai Lun papermaking, one of the four great inventions of ancient China.
30822525	1	19	theme	Broussonetia	156:167	arg1	papyrifera					169:178	Broussonetia papyrifera	156:178	Broussonetia papyrifera	156:178	Paper mulberry (Broussonetia papyrifera) is a well-known woody tree historically used for Cai Lun papermaking, one of the four great inventions of ancient China.
30822525	3	20	theme	genetic	653:659	arg1	maps					661:664	genetic maps	653:664	genetic maps	653:664	In this study, we obtained a chromosome-scale genome assembly for Paper mulberry using integrated approaches, including Illumina and PacBio sequencing platform as well as Hi-C, optical, and genetic maps.
30822525	9	21	theme	mulberry	1794:1801	arg1	usefulness					1774:1783	the usefulness	1770:1783	the usefulness of Paper mulberry in papermaking and barkcloth making	1770:1837	Our study provides significant insights into genetic basis of the usefulness of Paper mulberry in papermaking and barkcloth making, and as forage.
30822525	10	22	theme	worldwide	1974:1982	arg1	utilization					1944:1954	industrial utilization	1933:1954	domestication and selection as well as industrial utilization of Paper mulberry worldwide	1894:1982	These insights will facilitate further domestication and selection as well as industrial utilization of Paper mulberry worldwide.
30822525	10	22	theme	worldwide	1974:1982	arg1	selection					1912:1920	selection	1912:1920	selection	1912:1920	These insights will facilitate further domestication and selection as well as industrial utilization of Paper mulberry worldwide.
30822525	10	22	theme	worldwide	1974:1982	arg1	domestication					1894:1906	domestication	1894:1906	domestication	1894:1906	These insights will facilitate further domestication and selection as well as industrial utilization of Paper mulberry worldwide.
30822525	6	23	theme	increased	1084:1092	arg1	ratio					1094:1098	the increased ratio	1080:1098	the increased ratio of syringyl-lignin to guaiacyl-lignin in Paper mulberry	1080:1154	Moreover, the increased ratio of syringyl-lignin to guaiacyl-lignin in Paper mulberry underscores its suitability for use in medicine, forage, papermaking, and barkcloth making.
30822525	5	24	from	contraction	897:907	arg1	families					955:962	the flavonoid and lignin biosynthetic gene families	912:962	families	955:962	Comparative genomic analysis revealed the expansion and contraction in the flavonoid and lignin biosynthetic gene families, respectively, accounting for the enhanced flavonoid and decreased lignin biosynthesis in Paper mulberry.
30822525	5	24	from	contraction	897:907	arg1	flavonoid					916:924	the flavonoid and lignin biosynthetic gene families	912:962	flavonoid	916:924	Comparative genomic analysis revealed the expansion and contraction in the flavonoid and lignin biosynthetic gene families, respectively, accounting for the enhanced flavonoid and decreased lignin biosynthesis in Paper mulberry.
30822525	8	25	theme	Paper	1521:1525	arg1	mulberry					1527:1534	Paper mulberry	1521:1534	Paper mulberry	1521:1534	Collectively, these results suggest that Paper mulberry might have undergone adaptive evolution and recruited nitrogen-fixing microbes to promote growth by enhancing flavonoid production and altering lignin monomer composition.
30822525	0	26	theme	Chromosome-Scale	2:17	arg1	Assembly					26:33	A Chromosome-Scale Genome Assembly	0:33	A Chromosome-Scale Genome Assembly of Paper Mulberry	0:51	A Chromosome-Scale Genome Assembly of Paper Mulberry (Broussonetia papyrifera) Provides New Insights into Its Forage and Papermaking Usage.
30822525	5	27	theme	lignin	1031:1036	arg1	biosynthesis					1038:1049	decreased lignin biosynthesis	1021:1049	decreased lignin biosynthesis in Paper mulberry	1021:1067	Comparative genomic analysis revealed the expansion and contraction in the flavonoid and lignin biosynthetic gene families, respectively, accounting for the enhanced flavonoid and decreased lignin biosynthesis in Paper mulberry.
30822525	0	28	dep	Provides	79:86	arg1	papyrifera					67:76	papyrifera	67:76	papyrifera	67:76	A Chromosome-Scale Genome Assembly of Paper Mulberry (Broussonetia papyrifera) Provides New Insights into Its Forage and Papermaking Usage.
30822525	9	29	theme	usefulness	1774:1783	arg1	basis					1761:1765	genetic basis	1753:1765	genetic basis of the usefulness of Paper mulberry in papermaking and barkcloth making	1753:1837	Our study provides significant insights into genetic basis of the usefulness of Paper mulberry in papermaking and barkcloth making, and as forage.
30822525	4	30	theme	assembled	671:679	arg1	genome					696:701	The assembled Paper mulberry genome	667:701	The assembled Paper mulberry genome	667:701	The assembled Paper mulberry genome consists of 386.83 Mb, which is close to the estimated size, and 99.25% (383.93 Mb) of the assembly was assigned to 13 pseudochromosomes.
30822525	10	31	theme	mulberry	1965:1972	arg1	worldwide					1974:1982	Paper mulberry worldwide	1959:1982	Paper mulberry worldwide	1959:1982	These insights will facilitate further domestication and selection as well as industrial utilization of Paper mulberry worldwide.
30822525	2	32	theme	feedstuff	388:396	arg1	shortage					376:383	the shortage	372:383	the shortage of feedstuff	372:396	More recently, Paper mulberry has also been used as forage to address the shortage of feedstuff because of its digestible crude fiber and high protein contents.
30822525	10	33	theme	industrial	1933:1942	arg1	utilization					1944:1954	industrial utilization	1933:1954	domestication and selection as well as industrial utilization of Paper mulberry worldwide	1894:1982	These insights will facilitate further domestication and selection as well as industrial utilization of Paper mulberry worldwide.
30822525	5	34	theme	enhanced	998:1005	arg1	flavonoid					1007:1015	the enhanced flavonoid	994:1015	the enhanced flavonoid	994:1015	Comparative genomic analysis revealed the expansion and contraction in the flavonoid and lignin biosynthetic gene families, respectively, accounting for the enhanced flavonoid and decreased lignin biosynthesis in Paper mulberry.
30822525	5	35	theme	gene	950:953	arg1	families					955:962	the flavonoid and lignin biosynthetic gene families	912:962	families	955:962	Comparative genomic analysis revealed the expansion and contraction in the flavonoid and lignin biosynthetic gene families, respectively, accounting for the enhanced flavonoid and decreased lignin biosynthesis in Paper mulberry.
30822525	3	36	theme	chromosome-scale	492:507	arg1	assembly					516:523	a chromosome-scale genome assembly	490:523	a chromosome-scale genome assembly for Paper mulberry	490:542	In this study, we obtained a chromosome-scale genome assembly for Paper mulberry using integrated approaches, including Illumina and PacBio sequencing platform as well as Hi-C, optical, and genetic maps.
30822525	8	37	theme	monomer	1687:1693	arg1	composition					1695:1705	lignin monomer composition	1680:1705	lignin monomer composition	1680:1705	Collectively, these results suggest that Paper mulberry might have undergone adaptive evolution and recruited nitrogen-fixing microbes to promote growth by enhancing flavonoid production and altering lignin monomer composition.
30822525	0	38	theme	Genome	19:24	arg1	Assembly					26:33	A Chromosome-Scale Genome Assembly	0:33	A Chromosome-Scale Genome Assembly of Paper Mulberry	0:51	A Chromosome-Scale Genome Assembly of Paper Mulberry (Broussonetia papyrifera) Provides New Insights into Its Forage and Papermaking Usage.
30822525	6	39	theme	syringyl-lignin	1103:1117	arg1	ratio					1094:1098	the increased ratio	1080:1098	the increased ratio of syringyl-lignin to guaiacyl-lignin in Paper mulberry	1080:1154	Moreover, the increased ratio of syringyl-lignin to guaiacyl-lignin in Paper mulberry underscores its suitability for use in medicine, forage, papermaking, and barkcloth making.
30822525	5	40	theme	Comparative	841:851	arg1	analysis					861:868	Comparative genomic analysis	841:868	Comparative genomic analysis	841:868	Comparative genomic analysis revealed the expansion and contraction in the flavonoid and lignin biosynthetic gene families, respectively, accounting for the enhanced flavonoid and decreased lignin biosynthesis in Paper mulberry.
30822525	3	41	theme	sequencing	603:612	arg1	platform					614:621	Illumina and PacBio sequencing platform	583:621	Illumina and PacBio sequencing platform as well as Hi-C, optical, and genetic maps	583:664	In this study, we obtained a chromosome-scale genome assembly for Paper mulberry using integrated approaches, including Illumina and PacBio sequencing platform as well as Hi-C, optical, and genetic maps.
30822525	3	42	theme	genome	509:514	arg1	assembly					516:523	a chromosome-scale genome assembly	490:523	a chromosome-scale genome assembly for Paper mulberry	490:542	In this study, we obtained a chromosome-scale genome assembly for Paper mulberry using integrated approaches, including Illumina and PacBio sequencing platform as well as Hi-C, optical, and genetic maps.
30822525	1	43	theme	well-known	186:195	arg1	mulberry					146:153	Paper mulberry	140:153	Paper mulberry (Broussonetia papyrifera)	140:179	Paper mulberry (Broussonetia papyrifera) is a well-known woody tree historically used for Cai Lun papermaking, one of the four great inventions of ancient China.
30822525	1	43	theme	well-known	186:195	arg1	tree					203:206	a well-known woody tree	184:206	a well-known woody tree historically used for Cai Lun papermaking, one of the four great inventions of ancient China	184:299	Paper mulberry (Broussonetia papyrifera) is a well-known woody tree historically used for Cai Lun papermaking, one of the four great inventions of ancient China.
30822525	0	44	theme	Paper	38:42	arg1	Mulberry					44:51	Paper Mulberry	38:51	Paper Mulberry	38:51	A Chromosome-Scale Genome Assembly of Paper Mulberry (Broussonetia papyrifera) Provides New Insights into Its Forage and Papermaking Usage.
30822525	1	45	theme	great	267:271	arg1	inventions					273:282	the four great inventions	258:282	the four great inventions of ancient China	258:299	Paper mulberry (Broussonetia papyrifera) is a well-known woody tree historically used for Cai Lun papermaking, one of the four great inventions of ancient China.
30822525	5	46	from	biosynthesis	1038:1049	arg1	mulberry					1060:1067	Paper mulberry	1054:1067	Paper mulberry	1054:1067	Comparative genomic analysis revealed the expansion and contraction in the flavonoid and lignin biosynthetic gene families, respectively, accounting for the enhanced flavonoid and decreased lignin biosynthesis in Paper mulberry.
30822525	1	47	theme	woody	197:201	arg1	mulberry					146:153	Paper mulberry	140:153	Paper mulberry (Broussonetia papyrifera)	140:179	Paper mulberry (Broussonetia papyrifera) is a well-known woody tree historically used for Cai Lun papermaking, one of the four great inventions of ancient China.
30822525	1	47	theme	woody	197:201	arg1	tree					203:206	a well-known woody tree	184:206	a well-known woody tree historically used for Cai Lun papermaking, one of the four great inventions of ancient China	184:299	Paper mulberry (Broussonetia papyrifera) is a well-known woody tree historically used for Cai Lun papermaking, one of the four great inventions of ancient China.
30822525	2	48	theme	crude	424:428	arg1	fiber					430:434	its digestible crude fiber	409:434	its digestible crude fiber	409:434	More recently, Paper mulberry has also been used as forage to address the shortage of feedstuff because of its digestible crude fiber and high protein contents.
30822525	1	49	theme	inventions	273:282	arg1	papermaking					238:248	Cai Lun papermaking	230:248	Cai Lun papermaking	230:248	Paper mulberry (Broussonetia papyrifera) is a well-known woody tree historically used for Cai Lun papermaking, one of the four great inventions of ancient China.
30822525	1	49	theme	inventions	273:282	arg1	inventions					273:282	the four great inventions	258:282	the four great inventions of ancient China	258:299	Paper mulberry (Broussonetia papyrifera) is a well-known woody tree historically used for Cai Lun papermaking, one of the four great inventions of ancient China.
30822525	1	49	theme	inventions	273:282	arg1	one					251:253	one	251:253	one	251:253	Paper mulberry (Broussonetia papyrifera) is a well-known woody tree historically used for Cai Lun papermaking, one of the four great inventions of ancient China.
30822525	0	50	theme	Papermaking	121:131	arg1	Usage					133:137	Papermaking Usage	121:137	Papermaking Usage	121:137	A Chromosome-Scale Genome Assembly of Paper Mulberry (Broussonetia papyrifera) Provides New Insights into Its Forage and Papermaking Usage.
30822525	3	51	theme	integrated	550:559	arg1	platform					614:621	Illumina and PacBio sequencing platform	583:621	Illumina and PacBio sequencing platform as well as Hi-C, optical, and genetic maps	583:664	In this study, we obtained a chromosome-scale genome assembly for Paper mulberry using integrated approaches, including Illumina and PacBio sequencing platform as well as Hi-C, optical, and genetic maps.
30822525	3	51	theme	integrated	550:559	arg1	approaches					561:570	integrated approaches	550:570	integrated approaches	550:570	In this study, we obtained a chromosome-scale genome assembly for Paper mulberry using integrated approaches, including Illumina and PacBio sequencing platform as well as Hi-C, optical, and genetic maps.
30822525	3	51	theme	integrated	550:559	arg1	Hi-C					634:637	Hi-C	634:637	Hi-C	634:637	In this study, we obtained a chromosome-scale genome assembly for Paper mulberry using integrated approaches, including Illumina and PacBio sequencing platform as well as Hi-C, optical, and genetic maps.
30822525	7	52	theme	mulberry	1307:1314	arg1	microbiota					1287:1296	the root-associated microbiota	1267:1296	the root-associated microbiota of Paper mulberry	1267:1314	We also identified the root-associated microbiota of Paper mulberry and found that Pseudomonas and Rhizobia were enriched in its roots and may provide the source of nitrogen for its stems and leaves via symbiotic nitrogen fixation.
30822525	2	53	theme	digestible	413:422	arg1	fiber					430:434	its digestible crude fiber	409:434	its digestible crude fiber	409:434	More recently, Paper mulberry has also been used as forage to address the shortage of feedstuff because of its digestible crude fiber and high protein contents.
30822525	9	54	theme	Paper	1788:1792	arg1	mulberry					1794:1801	Paper mulberry	1788:1801	Paper mulberry	1788:1801	Our study provides significant insights into genetic basis of the usefulness of Paper mulberry in papermaking and barkcloth making, and as forage.
30822525	6	55	theme	making	1240:1245	arg1	barkcloth					1230:1238	barkcloth making	1230:1245	barkcloth making	1230:1245	Moreover, the increased ratio of syringyl-lignin to guaiacyl-lignin in Paper mulberry underscores its suitability for use in medicine, forage, papermaking, and barkcloth making.
30822525	3	56	theme	PacBio	596:601	arg1	platform					614:621	Illumina and PacBio sequencing platform	583:621	Illumina and PacBio sequencing platform as well as Hi-C, optical, and genetic maps	583:664	In this study, we obtained a chromosome-scale genome assembly for Paper mulberry using integrated approaches, including Illumina and PacBio sequencing platform as well as Hi-C, optical, and genetic maps.
30822525	3	57	theme	Paper	529:533	arg1	mulberry					535:542	Paper mulberry	529:542	Paper mulberry	529:542	In this study, we obtained a chromosome-scale genome assembly for Paper mulberry using integrated approaches, including Illumina and PacBio sequencing platform as well as Hi-C, optical, and genetic maps.
30822525	5	58	theme	decreased	1021:1029	arg1	biosynthesis					1038:1049	decreased lignin biosynthesis	1021:1049	decreased lignin biosynthesis in Paper mulberry	1021:1067	Comparative genomic analysis revealed the expansion and contraction in the flavonoid and lignin biosynthetic gene families, respectively, accounting for the enhanced flavonoid and decreased lignin biosynthesis in Paper mulberry.
30822525	5	59	dep	expansion	883:891	arg1	the					879:881	the	879:881	the	879:881	Comparative genomic analysis revealed the expansion and contraction in the flavonoid and lignin biosynthetic gene families, respectively, accounting for the enhanced flavonoid and decreased lignin biosynthesis in Paper mulberry.
30822525	0	60	theme	Mulberry	44:51	arg1	Assembly					26:33	A Chromosome-Scale Genome Assembly	0:33	A Chromosome-Scale Genome Assembly of Paper Mulberry	0:51	A Chromosome-Scale Genome Assembly of Paper Mulberry (Broussonetia papyrifera) Provides New Insights into Its Forage and Papermaking Usage.
30822525	1	61	theme	ancient	287:293	arg1	China					295:299	ancient China	287:299	ancient China	287:299	Paper mulberry (Broussonetia papyrifera) is a well-known woody tree historically used for Cai Lun papermaking, one of the four great inventions of ancient China.
30822525	9	62	from	papermaking	1806:1816	arg1	basis					1761:1765	genetic basis	1753:1765	genetic basis of the usefulness of Paper mulberry in papermaking and barkcloth making	1753:1837	Our study provides significant insights into genetic basis of the usefulness of Paper mulberry in papermaking and barkcloth making, and as forage.
30822525	4	63	theme	estimated	748:756	arg1	Mb					722:723	386.83 Mb	715:723	386.83 Mb	715:723	The assembled Paper mulberry genome consists of 386.83 Mb, which is close to the estimated size, and 99.25% (383.93 Mb) of the assembly was assigned to 13 pseudochromosomes.
30822525	4	63	theme	estimated	748:756	arg1	size					758:761	the estimated size	744:761	the estimated size	744:761	The assembled Paper mulberry genome consists of 386.83 Mb, which is close to the estimated size, and 99.25% (383.93 Mb) of the assembly was assigned to 13 pseudochromosomes.
30822525	1	64	theme	China	295:299	arg1	inventions					273:282	the four great inventions	258:282	the four great inventions of ancient China	258:299	Paper mulberry (Broussonetia papyrifera) is a well-known woody tree historically used for Cai Lun papermaking, one of the four great inventions of ancient China.
30822525	8	65	theme	adaptive	1557:1564	arg1	evolution					1566:1574	adaptive evolution	1557:1574	adaptive evolution	1557:1574	Collectively, these results suggest that Paper mulberry might have undergone adaptive evolution and recruited nitrogen-fixing microbes to promote growth by enhancing flavonoid production and altering lignin monomer composition.
30822525	10	66	theme	Paper	1959:1963	arg1	worldwide					1974:1982	Paper mulberry worldwide	1959:1982	Paper mulberry worldwide	1959:1982	These insights will facilitate further domestication and selection as well as industrial utilization of Paper mulberry worldwide.
30822525	6	67	theme	Paper	1141:1145	arg1	mulberry					1147:1154	Paper mulberry	1141:1154	Paper mulberry	1141:1154	Moreover, the increased ratio of syringyl-lignin to guaiacyl-lignin in Paper mulberry underscores its suitability for use in medicine, forage, papermaking, and barkcloth making.
30822525	5	68	theme	lignin	930:935	arg1	families					955:962	the flavonoid and lignin biosynthetic gene families	912:962	families	955:962	Comparative genomic analysis revealed the expansion and contraction in the flavonoid and lignin biosynthetic gene families, respectively, accounting for the enhanced flavonoid and decreased lignin biosynthesis in Paper mulberry.
30822525	9	69	from	basis	1761:1765	arg1	papermaking					1806:1816	papermaking	1806:1816	papermaking	1806:1816	Our study provides significant insights into genetic basis of the usefulness of Paper mulberry in papermaking and barkcloth making, and as forage.
30822525	9	69	from	basis	1761:1765	arg1	making					1832:1837	making	1832:1837	making	1832:1837	Our study provides significant insights into genetic basis of the usefulness of Paper mulberry in papermaking and barkcloth making, and as forage.
30822525	5	70	theme	genomic	853:859	arg1	analysis					861:868	Comparative genomic analysis	841:868	Comparative genomic analysis	841:868	Comparative genomic analysis revealed the expansion and contraction in the flavonoid and lignin biosynthetic gene families, respectively, accounting for the enhanced flavonoid and decreased lignin biosynthesis in Paper mulberry.
30822525	4	71	theme	assembly	794:801	arg1	assembly					794:801	the assembly	790:801	the assembly	790:801	The assembled Paper mulberry genome consists of 386.83 Mb, which is close to the estimated size, and 99.25% (383.93 Mb) of the assembly was assigned to 13 pseudochromosomes.
30822525	4	71	theme	assembly	794:801	arg1	%					773:773	99.25%	768:773	99.25% (383.93 Mb) of the assembly	768:801	The assembled Paper mulberry genome consists of 386.83 Mb, which is close to the estimated size, and 99.25% (383.93 Mb) of the assembly was assigned to 13 pseudochromosomes.
30822525	4	71	theme	assembly	794:801	arg1	Mb					783:784	383.93 Mb	776:784	383.93 Mb	776:784	The assembled Paper mulberry genome consists of 386.83 Mb, which is close to the estimated size, and 99.25% (383.93 Mb) of the assembly was assigned to 13 pseudochromosomes.
30822525	3	72	theme	Illumina	583:590	arg1	platform					614:621	Illumina and PacBio sequencing platform	583:621	Illumina and PacBio sequencing platform as well as Hi-C, optical, and genetic maps	583:664	In this study, we obtained a chromosome-scale genome assembly for Paper mulberry using integrated approaches, including Illumina and PacBio sequencing platform as well as Hi-C, optical, and genetic maps.
30822525	6	73	from	suitability	1172:1182	arg1	barkcloth					1230:1238	barkcloth making	1230:1245	barkcloth making	1230:1245	Moreover, the increased ratio of syringyl-lignin to guaiacyl-lignin in Paper mulberry underscores its suitability for use in medicine, forage, papermaking, and barkcloth making.
30822525	6	73	from	suitability	1172:1182	arg1	forage					1205:1210	forage	1205:1210	forage	1205:1210	Moreover, the increased ratio of syringyl-lignin to guaiacyl-lignin in Paper mulberry underscores its suitability for use in medicine, forage, papermaking, and barkcloth making.
30822525	6	73	from	suitability	1172:1182	arg1	medicine					1195:1202	medicine	1195:1202	medicine	1195:1202	Moreover, the increased ratio of syringyl-lignin to guaiacyl-lignin in Paper mulberry underscores its suitability for use in medicine, forage, papermaking, and barkcloth making.
30822525	6	73	from	suitability	1172:1182	arg1	papermaking					1213:1223	papermaking	1213:1223	papermaking	1213:1223	Moreover, the increased ratio of syringyl-lignin to guaiacyl-lignin in Paper mulberry underscores its suitability for use in medicine, forage, papermaking, and barkcloth making.
30822525	8	74	theme	lignin	1680:1685	arg1	composition					1695:1705	lignin monomer composition	1680:1705	lignin monomer composition	1680:1705	Collectively, these results suggest that Paper mulberry might have undergone adaptive evolution and recruited nitrogen-fixing microbes to promote growth by enhancing flavonoid production and altering lignin monomer composition.
30822525	5	75	from	flavonoid	1007:1015	arg1	mulberry					1060:1067	Paper mulberry	1054:1067	Paper mulberry	1054:1067	Comparative genomic analysis revealed the expansion and contraction in the flavonoid and lignin biosynthetic gene families, respectively, accounting for the enhanced flavonoid and decreased lignin biosynthesis in Paper mulberry.
30822525	9	76	dep	making	1832:1837	arg1	barkcloth					1822:1830	barkcloth	1822:1830	barkcloth	1822:1830	Our study provides significant insights into genetic basis of the usefulness of Paper mulberry in papermaking and barkcloth making, and as forage.
30822525	2	77	used	used	346:349	arg2	mulberry					323:330	Paper mulberry	317:330	Paper mulberry	317:330	More recently, Paper mulberry has also been used as forage to address the shortage of feedstuff because of its digestible crude fiber and high protein contents.
30822525	8	78	theme	nitrogen-fixing	1590:1604	arg1	microbes					1606:1613	recruited nitrogen-fixing microbes	1580:1613	recruited nitrogen-fixing microbes	1580:1613	Collectively, these results suggest that Paper mulberry might have undergone adaptive evolution and recruited nitrogen-fixing microbes to promote growth by enhancing flavonoid production and altering lignin monomer composition.
30822525	2	79	theme	protein	445:451	arg1	contents					453:460	high protein contents	440:460	high protein contents	440:460	More recently, Paper mulberry has also been used as forage to address the shortage of feedstuff because of its digestible crude fiber and high protein contents.
30822525	7	80	theme	nitrogen	1413:1420	arg1	source					1403:1408	the source	1399:1408	the source of nitrogen for its stems	1399:1434	We also identified the root-associated microbiota of Paper mulberry and found that Pseudomonas and Rhizobia were enriched in its roots and may provide the source of nitrogen for its stems and leaves via symbiotic nitrogen fixation.
30822525	7	81	theme	root-associated	1271:1285	arg1	microbiota					1287:1296	the root-associated microbiota	1267:1296	the root-associated microbiota of Paper mulberry	1267:1314	We also identified the root-associated microbiota of Paper mulberry and found that Pseudomonas and Rhizobia were enriched in its roots and may provide the source of nitrogen for its stems and leaves via symbiotic nitrogen fixation.
30822525	1	82	theme	Paper	140:144	arg1	mulberry					146:153	Paper mulberry	140:153	Paper mulberry (Broussonetia papyrifera)	140:179	Paper mulberry (Broussonetia papyrifera) is a well-known woody tree historically used for Cai Lun papermaking, one of the four great inventions of ancient China.
30822525	1	82	theme	Paper	140:144	arg1	papyrifera					169:178	Broussonetia papyrifera	156:178	Broussonetia papyrifera	156:178	Paper mulberry (Broussonetia papyrifera) is a well-known woody tree historically used for Cai Lun papermaking, one of the four great inventions of ancient China.
30822525	1	82	theme	Paper	140:144	arg1	tree					203:206	a well-known woody tree	184:206	a well-known woody tree historically used for Cai Lun papermaking, one of the four great inventions of ancient China	184:299	Paper mulberry (Broussonetia papyrifera) is a well-known woody tree historically used for Cai Lun papermaking, one of the four great inventions of ancient China.
30822525	5	83	from	expansion	883:891	arg1	families					955:962	the flavonoid and lignin biosynthetic gene families	912:962	families	955:962	Comparative genomic analysis revealed the expansion and contraction in the flavonoid and lignin biosynthetic gene families, respectively, accounting for the enhanced flavonoid and decreased lignin biosynthesis in Paper mulberry.
30822525	5	83	from	expansion	883:891	arg1	flavonoid					916:924	the flavonoid and lignin biosynthetic gene families	912:962	flavonoid	916:924	Comparative genomic analysis revealed the expansion and contraction in the flavonoid and lignin biosynthetic gene families, respectively, accounting for the enhanced flavonoid and decreased lignin biosynthesis in Paper mulberry.
30822525	1	84	theme	Cai	230:232	arg1	papermaking					238:248	Cai Lun papermaking	230:248	Cai Lun papermaking	230:248	Paper mulberry (Broussonetia papyrifera) is a well-known woody tree historically used for Cai Lun papermaking, one of the four great inventions of ancient China.
30822525	1	84	theme	Cai	230:232	arg1	inventions					273:282	the four great inventions	258:282	the four great inventions of ancient China	258:299	Paper mulberry (Broussonetia papyrifera) is a well-known woody tree historically used for Cai Lun papermaking, one of the four great inventions of ancient China.
30822525	1	84	theme	Cai	230:232	arg1	one					251:253	one	251:253	one	251:253	Paper mulberry (Broussonetia papyrifera) is a well-known woody tree historically used for Cai Lun papermaking, one of the four great inventions of ancient China.
30789325	10	0	theme	detected	1051:1058	arg1	phospholipids					1060:1072	The detected phospholipids	1047:1072	The detected phospholipids	1047:1072	The detected phospholipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylethanolamine-containing hydroxylated fatty acids and an unknown phospholipid.
30789325	10	0	theme	detected	1051:1058	arg1	diphosphatidylglycerol					1079:1100	diphosphatidylglycerol	1079:1100	diphosphatidylglycerol	1079:1100	The detected phospholipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylethanolamine-containing hydroxylated fatty acids and an unknown phospholipid.
30789325	1	1	from	soil	84:87	arg1	Thailand					92:99	Thailand	92:99	Thailand	92:99	nov., an actinomycete isolated from rhizosphere soil in Thailand.
30789325	4	2	theme	NBRC	569:572	arg1	16345T					574:579	Saccharopolyspora flava NBRC 16345T	545:579	Saccharopolyspora flava NBRC 16345T (97.5 %)	545:588	Phylogenetic analysis of the 16S rRNA gene sequences revealed that this isolate was most closely related to Saccharopolyspora tripterygii JCM 32123T (97.6 %), Saccharopolyspora dendranthemae NBRC 108675T (97.5 %) and Saccharopolyspora flava NBRC 16345T (97.5 %).
30789325	4	2	theme	NBRC	569:572	arg1	%					587:587	97.5 %	582:587	97.5 %	582:587	Phylogenetic analysis of the 16S rRNA gene sequences revealed that this isolate was most closely related to Saccharopolyspora tripterygii JCM 32123T (97.6 %), Saccharopolyspora dendranthemae NBRC 108675T (97.5 %) and Saccharopolyspora flava NBRC 16345T (97.5 %).
30789325	3	3	theme	polyphasic	307:316	arg1	approach					318:325	a polyphasic approach	305:325	a polyphasic approach	305:325	Strain H219T was characterised using a polyphasic approach.
30789325	4	4	theme	dendranthemae	505:517	arg1	108675T					524:530	Saccharopolyspora dendranthemae NBRC 108675T	487:530	Saccharopolyspora dendranthemae NBRC 108675T (97.5 %)	487:539	Phylogenetic analysis of the 16S rRNA gene sequences revealed that this isolate was most closely related to Saccharopolyspora tripterygii JCM 32123T (97.6 %), Saccharopolyspora dendranthemae NBRC 108675T (97.5 %) and Saccharopolyspora flava NBRC 16345T (97.5 %).
30789325	4	4	theme	dendranthemae	505:517	arg1	%					538:538	97.5 %	533:538	97.5 %	533:538	Phylogenetic analysis of the 16S rRNA gene sequences revealed that this isolate was most closely related to Saccharopolyspora tripterygii JCM 32123T (97.6 %), Saccharopolyspora dendranthemae NBRC 108675T (97.5 %) and Saccharopolyspora flava NBRC 16345T (97.5 %).
30789325	16	5	theme	=TBRC	1742:1746	arg1	H219T					1735:1739	H219T	1735:1739	H219T (=TBRC 8564T=NBRC 113388T)	1735:1766	The type strain is H219T (=TBRC 8564T=NBRC 113388T).
30789325	16	5	theme	=TBRC	1742:1746	arg1	113388T					1759:1765	=TBRC 8564T=NBRC 113388T	1742:1765	=TBRC 8564T=NBRC 113388T	1742:1765	The type strain is H219T (=TBRC 8564T=NBRC 113388T).
30789325	6	6	theme	H219T	783:787	arg1	peptidoglycan					759:771	The cell-wall peptidoglycan	745:771	The cell-wall peptidoglycan of strain H219T	745:787	The cell-wall peptidoglycan of strain H219T contained meso-diaminopimelic acid.
30789325	11	7	theme	70.6 mol	1286:1293	arg1	%					1294:1294	70.6 mol%	1286:1294	70.6 mol%	1286:1294	The DNA G+C content was 70.6 mol%.
30789325	11	7	theme	70.6 mol	1286:1293	arg1	G+C content					1270:1280	The DNA G+C content	1262:1280	The DNA G+C content	1262:1280	The DNA G+C content was 70.6 mol%.
30789325	10	8	theme	fatty	1221:1225	arg1	diphosphatidylglycerol					1079:1100	diphosphatidylglycerol	1079:1100	diphosphatidylglycerol	1079:1100	The detected phospholipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylethanolamine-containing hydroxylated fatty acids and an unknown phospholipid.
30789325	10	8	theme	fatty	1221:1225	arg1	acids					1227:1231	phosphatidylethanolamine-containing hydroxylated fatty acids	1172:1231	phosphatidylethanolamine-containing hydroxylated fatty acids	1172:1231	The detected phospholipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylethanolamine-containing hydroxylated fatty acids and an unknown phospholipid.
30789325	4	9	theme	Saccharopolyspora	436:452	arg1	32123T					470:475	Saccharopolyspora tripterygii JCM 32123T	436:475	Saccharopolyspora tripterygii JCM 32123T (97.6 %)	436:484	Phylogenetic analysis of the 16S rRNA gene sequences revealed that this isolate was most closely related to Saccharopolyspora tripterygii JCM 32123T (97.6 %), Saccharopolyspora dendranthemae NBRC 108675T (97.5 %) and Saccharopolyspora flava NBRC 16345T (97.5 %).
30789325	4	9	theme	Saccharopolyspora	436:452	arg1	%					483:483	97.6 %	478:483	97.6 %	478:483	Phylogenetic analysis of the 16S rRNA gene sequences revealed that this isolate was most closely related to Saccharopolyspora tripterygii JCM 32123T (97.6 %), Saccharopolyspora dendranthemae NBRC 108675T (97.5 %) and Saccharopolyspora flava NBRC 16345T (97.5 %).
30789325	4	10	theme	Saccharopolyspora	487:503	arg1	108675T					524:530	Saccharopolyspora dendranthemae NBRC 108675T	487:530	Saccharopolyspora dendranthemae NBRC 108675T (97.5 %)	487:539	Phylogenetic analysis of the 16S rRNA gene sequences revealed that this isolate was most closely related to Saccharopolyspora tripterygii JCM 32123T (97.6 %), Saccharopolyspora dendranthemae NBRC 108675T (97.5 %) and Saccharopolyspora flava NBRC 16345T (97.5 %).
30789325	4	10	theme	Saccharopolyspora	487:503	arg1	%					538:538	97.5 %	533:538	97.5 %	533:538	Phylogenetic analysis of the 16S rRNA gene sequences revealed that this isolate was most closely related to Saccharopolyspora tripterygii JCM 32123T (97.6 %), Saccharopolyspora dendranthemae NBRC 108675T (97.5 %) and Saccharopolyspora flava NBRC 16345T (97.5 %).
30789325	6	11	theme	strain	776:781	arg1	H219T					783:787	strain H219T	776:787	strain H219T	776:787	The cell-wall peptidoglycan of strain H219T contained meso-diaminopimelic acid.
30789325	14	12	theme	genus	1651:1655	arg1	Saccharopolyspora					1657:1673	the genus Saccharopolyspora	1647:1673	the genus Saccharopolyspora	1647:1673	Based on the data from the polyphasic studies, we propose that strain H219T is a novel species within the genus Saccharopolyspora, Saccharopolysporarhizosphaerae sp.
30789325	14	12	theme	genus	1651:1655	arg1	sp					1707:1708	Saccharopolysporarhizosphaerae sp	1676:1708	Saccharopolysporarhizosphaerae sp	1676:1708	Based on the data from the polyphasic studies, we propose that strain H219T is a novel species within the genus Saccharopolyspora, Saccharopolysporarhizosphaerae sp.
30789325	10	13	theme	hydroxylated	1208:1219	arg1	diphosphatidylglycerol					1079:1100	diphosphatidylglycerol	1079:1100	diphosphatidylglycerol	1079:1100	The detected phospholipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylethanolamine-containing hydroxylated fatty acids and an unknown phospholipid.
30789325	10	13	theme	hydroxylated	1208:1219	arg1	acids					1227:1231	phosphatidylethanolamine-containing hydroxylated fatty acids	1172:1231	phosphatidylethanolamine-containing hydroxylated fatty acids	1172:1231	The detected phospholipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylethanolamine-containing hydroxylated fatty acids and an unknown phospholipid.
30789325	5	14	theme	novel	692:696	arg1	isolate					698:704	the novel isolate	688:704	the novel isolate	688:704	However, DNA-DNA hybridization analyses showed a low relatedness in the range of 39-48 % between the novel isolate and the above closely related strains.
30789325	5	15	theme	low	640:642	arg1	relatedness					644:654	a low relatedness	638:654	a low relatedness in the range of 39-48 % between the novel isolate and the above closely related strains	638:742	However, DNA-DNA hybridization analyses showed a low relatedness in the range of 39-48 % between the novel isolate and the above closely related strains.
30789325	14	16	from	studies	1583:1589	arg1	data					1558:1561	the data	1554:1561	the data from the polyphasic studies	1554:1589	Based on the data from the polyphasic studies, we propose that strain H219T is a novel species within the genus Saccharopolyspora, Saccharopolysporarhizosphaerae sp.
30789325	7	17	theme	whole-cell	840:849	arg1	sugars					851:856	The diagnostic whole-cell sugars	825:856	The diagnostic whole-cell sugars	825:856	The diagnostic whole-cell sugars consisted of arabinose and galactose.
30789325	7	18	theme	diagnostic	829:838	arg1	sugars					851:856	The diagnostic whole-cell sugars	825:856	The diagnostic whole-cell sugars	825:856	The diagnostic whole-cell sugars consisted of arabinose and galactose.
30789325	9	19	theme	major	1015:1019	arg1	MK-9					1037:1040	MK-9	1037:1040	MK-9(H4)	1037:1044	The major menaquinone was MK-9(H4).
30789325	9	19	theme	major	1015:1019	arg1	menaquinone					1021:1031	The major menaquinone	1011:1031	The major menaquinone	1011:1031	The major menaquinone was MK-9(H4).
30789325	13	20	theme	several	1514:1520	arg1	properties					1533:1542	several phenotypic properties	1514:1542	several phenotypic properties	1514:1542	However, strain H219T could be distinguished from closely related strains by several phenotypic properties.
30789325	1	21	theme	rhizosphere	72:82	arg1	soil					84:87	rhizosphere soil	72:87	rhizosphere soil in Thailand	72:99	nov., an actinomycete isolated from rhizosphere soil in Thailand.
30789325	10	22	theme	phosphatidylethanolamine-containing	1172:1206	arg1	diphosphatidylglycerol					1079:1100	diphosphatidylglycerol	1079:1100	diphosphatidylglycerol	1079:1100	The detected phospholipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylethanolamine-containing hydroxylated fatty acids and an unknown phospholipid.
30789325	10	22	theme	phosphatidylethanolamine-containing	1172:1206	arg1	acids					1227:1231	phosphatidylethanolamine-containing hydroxylated fatty acids	1172:1231	phosphatidylethanolamine-containing hydroxylated fatty acids	1172:1231	The detected phospholipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylethanolamine-containing hydroxylated fatty acids and an unknown phospholipid.
30789325	2	23	theme	strain	138:143	arg1	H219T					145:149	strain H219T	138:149	strain H219T	138:149	A novel actinomycete, designated as strain H219T, was isolated from rhizosphere soil collected under an Elephant ear plant (Colocasiaesculenta) in Bangkok, Thailand.
30789325	4	24	theme	rRNA	361:364	arg1	sequences					371:379	the 16S rRNA gene sequences	353:379	the 16S rRNA gene sequences	353:379	Phylogenetic analysis of the 16S rRNA gene sequences revealed that this isolate was most closely related to Saccharopolyspora tripterygii JCM 32123T (97.6 %), Saccharopolyspora dendranthemae NBRC 108675T (97.5 %) and Saccharopolyspora flava NBRC 16345T (97.5 %).
30789325	16	25	theme	8564T=NBRC	1748:1757	arg1	H219T					1735:1739	H219T	1735:1739	H219T (=TBRC 8564T=NBRC 113388T)	1735:1766	The type strain is H219T (=TBRC 8564T=NBRC 113388T).
30789325	16	25	theme	8564T=NBRC	1748:1757	arg1	113388T					1759:1765	=TBRC 8564T=NBRC 113388T	1742:1765	=TBRC 8564T=NBRC 113388T	1742:1765	The type strain is H219T (=TBRC 8564T=NBRC 113388T).
30789325	12	26	with	consistent	1381:1390	arg1	members					1397:1403	members	1397:1403	members of the genus Saccharopolyspora	1397:1434	Strain H219T represented chemotaxonomic and morphological characteristics that were consistent with members of the genus Saccharopolyspora.
30789325	12	27	theme	Strain	1297:1302	arg1	H219T					1304:1308	Strain H219T	1297:1308	Strain H219T	1297:1308	Strain H219T represented chemotaxonomic and morphological characteristics that were consistent with members of the genus Saccharopolyspora.
30789325	0	28	theme	rhizosphaerae	18:30	arg1	sp					32:33	Saccharopolyspora rhizosphaerae sp	0:33	Saccharopolyspora rhizosphaerae sp.	0:34	Saccharopolyspora rhizosphaerae sp.
30789325	5	29	theme	related	728:734	arg1	strains					736:742	the above closely related strains	710:742	the above closely related strains	710:742	However, DNA-DNA hybridization analyses showed a low relatedness in the range of 39-48 % between the novel isolate and the above closely related strains.
30789325	5	30	theme	DNA-DNA	600:606	arg1	analyses					622:629	DNA-DNA hybridization analyses	600:629	DNA-DNA hybridization analyses	600:629	However, DNA-DNA hybridization analyses showed a low relatedness in the range of 39-48 % between the novel isolate and the above closely related strains.
30789325	4	31	theme	16S	357:359	arg1	sequences					371:379	the 16S rRNA gene sequences	353:379	the 16S rRNA gene sequences	353:379	Phylogenetic analysis of the 16S rRNA gene sequences revealed that this isolate was most closely related to Saccharopolyspora tripterygii JCM 32123T (97.6 %), Saccharopolyspora dendranthemae NBRC 108675T (97.5 %) and Saccharopolyspora flava NBRC 16345T (97.5 %).
30789325	2	32	from	plant	219:223	arg1	Thailand					258:265	Thailand	258:265	Thailand	258:265	A novel actinomycete, designated as strain H219T, was isolated from rhizosphere soil collected under an Elephant ear plant (Colocasiaesculenta) in Bangkok, Thailand.
30789325	12	33	theme	chemotaxonomic	1322:1335	arg1	characteristics					1355:1369	chemotaxonomic and morphological characteristics	1322:1369	chemotaxonomic and morphological characteristics that were consistent with members of the genus Saccharopolyspora	1322:1434	Strain H219T represented chemotaxonomic and morphological characteristics that were consistent with members of the genus Saccharopolyspora.
30789325	0	34	theme	Saccharopolyspora	0:16	arg1	sp					32:33	Saccharopolyspora rhizosphaerae sp	0:33	Saccharopolyspora rhizosphaerae sp.	0:34	Saccharopolyspora rhizosphaerae sp.
30789325	5	35	theme	hybridization	608:620	arg1	analyses					622:629	DNA-DNA hybridization analyses	600:629	DNA-DNA hybridization analyses	600:629	However, DNA-DNA hybridization analyses showed a low relatedness in the range of 39-48 % between the novel isolate and the above closely related strains.
30789325	4	36	theme	sequences	371:379	arg1	analysis					341:348	Phylogenetic analysis	328:348	Phylogenetic analysis of the 16S rRNA gene sequences	328:379	Phylogenetic analysis of the 16S rRNA gene sequences revealed that this isolate was most closely related to Saccharopolyspora tripterygii JCM 32123T (97.6 %), Saccharopolyspora dendranthemae NBRC 108675T (97.5 %) and Saccharopolyspora flava NBRC 16345T (97.5 %).
30789325	1	37	attach	isolated	58:65	arg2	actinomycete					45:56	an actinomycete	42:56	an actinomycete isolated from rhizosphere soil in Thailand	42:99	nov., an actinomycete isolated from rhizosphere soil in Thailand.
30789325	1	37	attach	isolated	58:65	arg1	soil					84:87	rhizosphere soil	72:87	rhizosphere soil in Thailand	72:99	nov., an actinomycete isolated from rhizosphere soil in Thailand.
30789325	4	38	theme	gene	366:369	arg1	sequences					371:379	the 16S rRNA gene sequences	353:379	the 16S rRNA gene sequences	353:379	Phylogenetic analysis of the 16S rRNA gene sequences revealed that this isolate was most closely related to Saccharopolyspora tripterygii JCM 32123T (97.6 %), Saccharopolyspora dendranthemae NBRC 108675T (97.5 %) and Saccharopolyspora flava NBRC 16345T (97.5 %).
30789325	12	39	theme	Saccharopolyspora	1418:1434	arg1	members					1397:1403	members	1397:1403	members of the genus Saccharopolyspora	1397:1434	Strain H219T represented chemotaxonomic and morphological characteristics that were consistent with members of the genus Saccharopolyspora.
30789325	4	40	theme	Saccharopolyspora	545:561	arg1	16345T					574:579	Saccharopolyspora flava NBRC 16345T	545:579	Saccharopolyspora flava NBRC 16345T (97.5 %)	545:588	Phylogenetic analysis of the 16S rRNA gene sequences revealed that this isolate was most closely related to Saccharopolyspora tripterygii JCM 32123T (97.6 %), Saccharopolyspora dendranthemae NBRC 108675T (97.5 %) and Saccharopolyspora flava NBRC 16345T (97.5 %).
30789325	4	40	theme	Saccharopolyspora	545:561	arg1	%					587:587	97.5 %	582:587	97.5 %	582:587	Phylogenetic analysis of the 16S rRNA gene sequences revealed that this isolate was most closely related to Saccharopolyspora tripterygii JCM 32123T (97.6 %), Saccharopolyspora dendranthemae NBRC 108675T (97.5 %) and Saccharopolyspora flava NBRC 16345T (97.5 %).
30789325	3	41	theme	Strain	268:273	arg1	H219T					275:279	Strain H219T	268:279	Strain H219T	268:279	Strain H219T was characterised using a polyphasic approach.
30789325	2	42	theme	rhizosphere	170:180	arg1	soil					182:185	rhizosphere soil	170:185	rhizosphere soil collected under an Elephant ear plant (Colocasiaesculenta) in Bangkok, Thailand	170:265	A novel actinomycete, designated as strain H219T, was isolated from rhizosphere soil collected under an Elephant ear plant (Colocasiaesculenta) in Bangkok, Thailand.
30789325	12	43	theme	genus	1412:1416	arg1	Saccharopolyspora					1418:1434	the genus Saccharopolyspora	1408:1434	the genus Saccharopolyspora	1408:1434	Strain H219T represented chemotaxonomic and morphological characteristics that were consistent with members of the genus Saccharopolyspora.
30789325	8	44	theme	cellular	900:907	arg1	profile					920:926	The cellular fatty acid profile	896:926	The cellular fatty acid profile mainly	896:933	The cellular fatty acid profile mainly comprised iso-C16 : 0, anteiso-C17 : 0, iso-C15 : 0, and 10-methyl C17 : 0.
30789325	1	45	dep	actinomycete	45:56	arg1	nov.					36:39	nov.	36:39	nov.	36:39	nov., an actinomycete isolated from rhizosphere soil in Thailand.
30789325	13	46	theme	strain	1446:1451	arg1	H219T					1453:1457	strain H219T	1446:1457	strain H219T	1446:1457	However, strain H219T could be distinguished from closely related strains by several phenotypic properties.
30789325	5	47	theme	%	678:678	arg1	range					663:667	the range	659:667	the range of 39-48 %	659:678	However, DNA-DNA hybridization analyses showed a low relatedness in the range of 39-48 % between the novel isolate and the above closely related strains.
30789325	13	48	theme	phenotypic	1522:1531	arg1	properties					1533:1542	several phenotypic properties	1514:1542	several phenotypic properties	1514:1542	However, strain H219T could be distinguished from closely related strains by several phenotypic properties.
30789325	4	49	theme	flava	563:567	arg1	16345T					574:579	Saccharopolyspora flava NBRC 16345T	545:579	Saccharopolyspora flava NBRC 16345T (97.5 %)	545:588	Phylogenetic analysis of the 16S rRNA gene sequences revealed that this isolate was most closely related to Saccharopolyspora tripterygii JCM 32123T (97.6 %), Saccharopolyspora dendranthemae NBRC 108675T (97.5 %) and Saccharopolyspora flava NBRC 16345T (97.5 %).
30789325	4	49	theme	flava	563:567	arg1	%					587:587	97.5 %	582:587	97.5 %	582:587	Phylogenetic analysis of the 16S rRNA gene sequences revealed that this isolate was most closely related to Saccharopolyspora tripterygii JCM 32123T (97.6 %), Saccharopolyspora dendranthemae NBRC 108675T (97.5 %) and Saccharopolyspora flava NBRC 16345T (97.5 %).
30789325	4	50	theme	JCM	466:468	arg1	32123T					470:475	Saccharopolyspora tripterygii JCM 32123T	436:475	Saccharopolyspora tripterygii JCM 32123T (97.6 %)	436:484	Phylogenetic analysis of the 16S rRNA gene sequences revealed that this isolate was most closely related to Saccharopolyspora tripterygii JCM 32123T (97.6 %), Saccharopolyspora dendranthemae NBRC 108675T (97.5 %) and Saccharopolyspora flava NBRC 16345T (97.5 %).
30789325	4	50	theme	JCM	466:468	arg1	%					483:483	97.6 %	478:483	97.6 %	478:483	Phylogenetic analysis of the 16S rRNA gene sequences revealed that this isolate was most closely related to Saccharopolyspora tripterygii JCM 32123T (97.6 %), Saccharopolyspora dendranthemae NBRC 108675T (97.5 %) and Saccharopolyspora flava NBRC 16345T (97.5 %).
30789325	8	51	theme	acid	915:918	arg1	profile					920:926	The cellular fatty acid profile	896:926	The cellular fatty acid profile mainly	896:933	The cellular fatty acid profile mainly comprised iso-C16 : 0, anteiso-C17 : 0, iso-C15 : 0, and 10-methyl C17 : 0.
30789325	2	52	attach	isolated	156:163	arg1	soil					182:185	rhizosphere soil	170:185	rhizosphere soil collected under an Elephant ear plant (Colocasiaesculenta) in Bangkok, Thailand	170:265	A novel actinomycete, designated as strain H219T, was isolated from rhizosphere soil collected under an Elephant ear plant (Colocasiaesculenta) in Bangkok, Thailand.
30789325	2	52	attach	isolated	156:163	arg2	actinomycete					110:121	A novel actinomycete	102:121	A novel actinomycete	102:121	A novel actinomycete, designated as strain H219T, was isolated from rhizosphere soil collected under an Elephant ear plant (Colocasiaesculenta) in Bangkok, Thailand.
30789325	4	53	theme	NBRC	519:522	arg1	108675T					524:530	Saccharopolyspora dendranthemae NBRC 108675T	487:530	Saccharopolyspora dendranthemae NBRC 108675T (97.5 %)	487:539	Phylogenetic analysis of the 16S rRNA gene sequences revealed that this isolate was most closely related to Saccharopolyspora tripterygii JCM 32123T (97.6 %), Saccharopolyspora dendranthemae NBRC 108675T (97.5 %) and Saccharopolyspora flava NBRC 16345T (97.5 %).
30789325	4	53	theme	NBRC	519:522	arg1	%					538:538	97.5 %	533:538	97.5 %	533:538	Phylogenetic analysis of the 16S rRNA gene sequences revealed that this isolate was most closely related to Saccharopolyspora tripterygii JCM 32123T (97.6 %), Saccharopolyspora dendranthemae NBRC 108675T (97.5 %) and Saccharopolyspora flava NBRC 16345T (97.5 %).
30789325	11	54	theme	DNA	1266:1268	arg1	%					1294:1294	70.6 mol%	1286:1294	70.6 mol%	1286:1294	The DNA G+C content was 70.6 mol%.
30789325	11	54	theme	DNA	1266:1268	arg1	G+C content					1270:1280	The DNA G+C content	1262:1280	The DNA G+C content	1262:1280	The DNA G+C content was 70.6 mol%.
30789325	4	55	theme	tripterygii	454:464	arg1	32123T					470:475	Saccharopolyspora tripterygii JCM 32123T	436:475	Saccharopolyspora tripterygii JCM 32123T (97.6 %)	436:484	Phylogenetic analysis of the 16S rRNA gene sequences revealed that this isolate was most closely related to Saccharopolyspora tripterygii JCM 32123T (97.6 %), Saccharopolyspora dendranthemae NBRC 108675T (97.5 %) and Saccharopolyspora flava NBRC 16345T (97.5 %).
30789325	4	55	theme	tripterygii	454:464	arg1	%					483:483	97.6 %	478:483	97.6 %	478:483	Phylogenetic analysis of the 16S rRNA gene sequences revealed that this isolate was most closely related to Saccharopolyspora tripterygii JCM 32123T (97.6 %), Saccharopolyspora dendranthemae NBRC 108675T (97.5 %) and Saccharopolyspora flava NBRC 16345T (97.5 %).
30789325	2	56	theme	ear	215:217	arg1	plant					219:223	an Elephant ear plant	203:223	an Elephant ear plant (Colocasiaesculenta) in Bangkok, Thailand	203:265	A novel actinomycete, designated as strain H219T, was isolated from rhizosphere soil collected under an Elephant ear plant (Colocasiaesculenta) in Bangkok, Thailand.
30789325	2	56	theme	ear	215:217	arg1	Colocasiaesculenta					226:243	Colocasiaesculenta	226:243	Colocasiaesculenta	226:243	A novel actinomycete, designated as strain H219T, was isolated from rhizosphere soil collected under an Elephant ear plant (Colocasiaesculenta) in Bangkok, Thailand.
30789325	4	57	theme	Phylogenetic	328:339	arg1	analysis					341:348	Phylogenetic analysis	328:348	Phylogenetic analysis of the 16S rRNA gene sequences	328:379	Phylogenetic analysis of the 16S rRNA gene sequences revealed that this isolate was most closely related to Saccharopolyspora tripterygii JCM 32123T (97.6 %), Saccharopolyspora dendranthemae NBRC 108675T (97.5 %) and Saccharopolyspora flava NBRC 16345T (97.5 %).
30789325	2	58	theme	novel	104:108	arg1	actinomycete					110:121	A novel actinomycete	102:121	A novel actinomycete	102:121	A novel actinomycete, designated as strain H219T, was isolated from rhizosphere soil collected under an Elephant ear plant (Colocasiaesculenta) in Bangkok, Thailand.
30789325	2	59	theme	Elephant	206:213	arg1	plant					219:223	an Elephant ear plant	203:223	an Elephant ear plant (Colocasiaesculenta) in Bangkok, Thailand	203:265	A novel actinomycete, designated as strain H219T, was isolated from rhizosphere soil collected under an Elephant ear plant (Colocasiaesculenta) in Bangkok, Thailand.
30789325	2	59	theme	Elephant	206:213	arg1	Colocasiaesculenta					226:243	Colocasiaesculenta	226:243	Colocasiaesculenta	226:243	A novel actinomycete, designated as strain H219T, was isolated from rhizosphere soil collected under an Elephant ear plant (Colocasiaesculenta) in Bangkok, Thailand.
30789325	14	60	theme	novel	1626:1630	arg1	H219T					1615:1619	strain H219T	1608:1619	strain H219T	1608:1619	Based on the data from the polyphasic studies, we propose that strain H219T is a novel species within the genus Saccharopolyspora, Saccharopolysporarhizosphaerae sp.
30789325	14	60	theme	novel	1626:1630	arg1	species					1632:1638	a novel species	1624:1638	a novel species within the genus Saccharopolyspora, Saccharopolysporarhizosphaerae sp	1624:1708	Based on the data from the polyphasic studies, we propose that strain H219T is a novel species within the genus Saccharopolyspora, Saccharopolysporarhizosphaerae sp.
30789325	8	61	theme	fatty	909:913	arg1	profile					920:926	The cellular fatty acid profile	896:926	The cellular fatty acid profile mainly	896:933	The cellular fatty acid profile mainly comprised iso-C16 : 0, anteiso-C17 : 0, iso-C15 : 0, and 10-methyl C17 : 0.
30789325	6	62	theme	cell-wall	749:757	arg1	peptidoglycan					759:771	The cell-wall peptidoglycan	745:771	The cell-wall peptidoglycan of strain H219T	745:787	The cell-wall peptidoglycan of strain H219T contained meso-diaminopimelic acid.
30789325	12	63	theme	morphological	1341:1353	arg1	characteristics					1355:1369	chemotaxonomic and morphological characteristics	1322:1369	chemotaxonomic and morphological characteristics that were consistent with members of the genus Saccharopolyspora	1322:1434	Strain H219T represented chemotaxonomic and morphological characteristics that were consistent with members of the genus Saccharopolyspora.
30789325	14	64	theme	polyphasic	1572:1581	arg1	studies					1583:1589	the polyphasic studies	1568:1589	the polyphasic studies	1568:1589	Based on the data from the polyphasic studies, we propose that strain H219T is a novel species within the genus Saccharopolyspora, Saccharopolysporarhizosphaerae sp.
30789325	13	65	theme	related	1495:1501	arg1	strains					1503:1509	closely related strains	1487:1509	closely related strains	1487:1509	However, strain H219T could be distinguished from closely related strains by several phenotypic properties.
30789325	10	66	theme	unknown	1240:1246	arg1	phospholipid					1248:1259	an unknown phospholipid	1237:1259	an unknown phospholipid	1237:1259	The detected phospholipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylethanolamine-containing hydroxylated fatty acids and an unknown phospholipid.
30789325	10	66	theme	unknown	1240:1246	arg1	diphosphatidylglycerol					1079:1100	diphosphatidylglycerol	1079:1100	diphosphatidylglycerol	1079:1100	The detected phospholipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylethanolamine-containing hydroxylated fatty acids and an unknown phospholipid.
30789325	6	67	theme	meso-diaminopimelic	799:817	arg1	acid					819:822	meso-diaminopimelic acid	799:822	meso-diaminopimelic acid	799:822	The cell-wall peptidoglycan of strain H219T contained meso-diaminopimelic acid.
30789325	8	68	dep	iso-C16 	945:952	arg1	 0					971:972	 0	971:972	 0	971:972	The cellular fatty acid profile mainly comprised iso-C16 : 0, anteiso-C17 : 0, iso-C15 : 0, and 10-methyl C17 : 0.
30789325	8	68	dep	iso-C16 	945:952	arg1	10-methyl					992:1000	10-methyl	992:1000	10-methyl	992:1000	The cellular fatty acid profile mainly comprised iso-C16 : 0, anteiso-C17 : 0, iso-C15 : 0, and 10-methyl C17 : 0.
30789325	8	68	dep	iso-C16 	945:952	arg1	C17 					1002:1005	C17 	1002:1005	C17 	1002:1005	The cellular fatty acid profile mainly comprised iso-C16 : 0, anteiso-C17 : 0, iso-C15 : 0, and 10-methyl C17 : 0.
30789325	8	68	dep	iso-C16 	945:952	arg1	 0					954:955	 0	954:955	 0	954:955	The cellular fatty acid profile mainly comprised iso-C16 : 0, anteiso-C17 : 0, iso-C15 : 0, and 10-methyl C17 : 0.
30789325	8	68	dep	iso-C16 	945:952	arg1	 0					984:985	 0	984:985	 0	984:985	The cellular fatty acid profile mainly comprised iso-C16 : 0, anteiso-C17 : 0, iso-C15 : 0, and 10-methyl C17 : 0.
30789325	8	68	dep	iso-C16 	945:952	arg1	 0					1007:1008	 0	1007:1008	 0	1007:1008	The cellular fatty acid profile mainly comprised iso-C16 : 0, anteiso-C17 : 0, iso-C15 : 0, and 10-methyl C17 : 0.
30789325	8	68	dep	iso-C16 	945:952	arg1	anteiso-C17 					958:969	anteiso-C17 	958:969	anteiso-C17 	958:969	The cellular fatty acid profile mainly comprised iso-C16 : 0, anteiso-C17 : 0, iso-C15 : 0, and 10-methyl C17 : 0.
30789325	14	69	theme	Saccharopolysporarhizosphaerae	1676:1705	arg1	Saccharopolyspora					1657:1673	the genus Saccharopolyspora	1647:1673	the genus Saccharopolyspora	1647:1673	Based on the data from the polyphasic studies, we propose that strain H219T is a novel species within the genus Saccharopolyspora, Saccharopolysporarhizosphaerae sp.
30789325	14	69	theme	Saccharopolysporarhizosphaerae	1676:1705	arg1	sp					1707:1708	Saccharopolysporarhizosphaerae sp	1676:1708	Saccharopolysporarhizosphaerae sp	1676:1708	Based on the data from the polyphasic studies, we propose that strain H219T is a novel species within the genus Saccharopolyspora, Saccharopolysporarhizosphaerae sp.
30789325	6	70	contain	contained	789:797	arg1	peptidoglycan					759:771	The cell-wall peptidoglycan	745:771	The cell-wall peptidoglycan of strain H219T	745:787	The cell-wall peptidoglycan of strain H219T contained meso-diaminopimelic acid.
30789325	6	70	contain	contained	789:797	arg2	acid					819:822	meso-diaminopimelic acid	799:822	meso-diaminopimelic acid	799:822	The cell-wall peptidoglycan of strain H219T contained meso-diaminopimelic acid.
30789325	5	71	from	relatedness	644:654	arg1	range					663:667	the range	659:667	the range of 39-48 %	659:678	However, DNA-DNA hybridization analyses showed a low relatedness in the range of 39-48 % between the novel isolate and the above closely related strains.
30789325	5	72	theme	above	714:718	arg1	strains					736:742	the above closely related strains	710:742	the above closely related strains	710:742	However, DNA-DNA hybridization analyses showed a low relatedness in the range of 39-48 % between the novel isolate and the above closely related strains.
30789325	16	73	theme	type	1720:1723	arg1	H219T					1735:1739	H219T	1735:1739	H219T (=TBRC 8564T=NBRC 113388T)	1735:1766	The type strain is H219T (=TBRC 8564T=NBRC 113388T).
30789325	16	73	theme	type	1720:1723	arg1	strain					1725:1730	The type strain	1716:1730	The type strain	1716:1730	The type strain is H219T (=TBRC 8564T=NBRC 113388T).
30789325	14	74	theme	strain	1608:1613	arg1	H219T					1615:1619	strain H219T	1608:1619	strain H219T	1608:1619	Based on the data from the polyphasic studies, we propose that strain H219T is a novel species within the genus Saccharopolyspora, Saccharopolysporarhizosphaerae sp.
30789325	14	74	theme	strain	1608:1613	arg1	species					1632:1638	a novel species	1624:1638	a novel species within the genus Saccharopolyspora, Saccharopolysporarhizosphaerae sp	1624:1708	Based on the data from the polyphasic studies, we propose that strain H219T is a novel species within the genus Saccharopolyspora, Saccharopolysporarhizosphaerae sp.
30300120	6	0	theme	rRNA	497:500	arg1	sequences					507:515	16S rRNA gene sequences	493:515	16S rRNA gene sequences	493:515	Analysis of 16S rRNA gene sequences revealed that strain SCSIO 08198T had the highest similarity of 97.2 % to Rubrobacter radiotolerans DSM 5868T, and loosely related (<94.2 %) to all other species in the genus Rubrobacter.
30300120	2	1	theme	mesophilic	108:117	arg1	strain					142:147	A novel mesophilic marine actinobacterial strain	100:147	A novel mesophilic marine actinobacterial strain	100:147	A novel mesophilic marine actinobacterial strain, designated as SCSIO 08198T, was isolated from a deep-sea sediment sample collected from the Indian Ocean.
30300120	13	2	theme	new	1317:1319	arg1	species					1321:1327	a new species	1315:1327	a new species	1315:1327	Based on these polyphasic data, a new species, Rubrobacterindicoceani sp.
30300120	8	3	theme	fatty	883:887	arg1	profile					894:900	The total cellular fatty acid profile	864:900	The total cellular fatty acid profile	864:900	The total cellular fatty acid profile was dominated by C16 : 0 12-methyl.
30300120	13	4	theme	Rubrobacterindicoceani	1330:1351	arg1	sp					1353:1354	Rubrobacterindicoceani sp	1330:1354	Rubrobacterindicoceani sp	1330:1354	Based on these polyphasic data, a new species, Rubrobacterindicoceani sp.
30300120	12	5	theme	circular	1245:1252	arg1	chromosome					1254:1263	one circular chromosome	1241:1263	one circular chromosome	1241:1263	Based on the whole genome sequence analysis, the genome size is 3 078 689 bp with DNA G+C value of 63.8 mol%, including one circular chromosome and two plasmids.
30300120	6	6	theme	16S	493:495	arg1	sequences					507:515	16S rRNA gene sequences	493:515	16S rRNA gene sequences	493:515	Analysis of 16S rRNA gene sequences revealed that strain SCSIO 08198T had the highest similarity of 97.2 % to Rubrobacter radiotolerans DSM 5868T, and loosely related (<94.2 %) to all other species in the genus Rubrobacter.
30300120	2	7	theme	novel	102:106	arg1	strain					142:147	A novel mesophilic marine actinobacterial strain	100:147	A novel mesophilic marine actinobacterial strain	100:147	A novel mesophilic marine actinobacterial strain, designated as SCSIO 08198T, was isolated from a deep-sea sediment sample collected from the Indian Ocean.
30300120	14	8	theme	105148T=CGMCC	1416:1428	arg1	1.16398T					1430:1437	=DSM 105148T=CGMCC 1.16398T	1411:1437	=DSM 105148T=CGMCC 1.16398T	1411:1437	nov., is proposed, with the type strain SCSIO 08198T (=DSM 105148T=CGMCC 1.16398T).
30300120	14	8	theme	105148T=CGMCC	1416:1428	arg1	08198T					1403:1408	the type strain SCSIO 08198T	1381:1408	the type strain SCSIO 08198T (=DSM 105148T=CGMCC 1.16398T)	1381:1438	nov., is proposed, with the type strain SCSIO 08198T (=DSM 105148T=CGMCC 1.16398T).
30300120	4	9	theme	%	375:375	arg1	NaCl					383:386	1-5 % (w/v) NaCl	371:386	1-5 % (w/v) NaCl	371:386	Good growth occurred on marine agar with 1-5 % (w/v) NaCl and incubation at 28 °C for more than a fortnight.
30300120	4	10	dep	%	375:375	arg1	w/v					378:380	w/v	378:380	w/v	378:380	Good growth occurred on marine agar with 1-5 % (w/v) NaCl and incubation at 28 °C for more than a fortnight.
30300120	11	11	theme	major	1019:1023	arg1	phospholipids					1025:1037	The major phospholipids	1015:1037	The major phospholipids	1015:1037	The major phospholipids were diphosphatidylglycerol, phosphatidylglycerol and unidentified phospholipids.
30300120	11	11	theme	major	1019:1023	arg1	diphosphatidylglycerol					1044:1065	diphosphatidylglycerol	1044:1065	diphosphatidylglycerol	1044:1065	The major phospholipids were diphosphatidylglycerol, phosphatidylglycerol and unidentified phospholipids.
30300120	7	12	with	lineage	817:823	arg1	members					830:836	members	830:836	members of the genus Rubrobacter	830:861	Phylogenetic analysis based on nearly complete 16S rRNA gene sequences revealed that the novel isolate shared a lineage with members of the genus Rubrobacter.
30300120	4	13	theme	1-5 	371:374	arg1	%					375:375	%	375:375	%	375:375	Good growth occurred on marine agar with 1-5 % (w/v) NaCl and incubation at 28 °C for more than a fortnight.
30300120	1	14	theme	marine	40:45	arg1	actinobacterium					47:61	a new marine actinobacterium	34:61	a new marine actinobacterium isolated from Indian Ocean sediment	34:97	nov., a new marine actinobacterium isolated from Indian Ocean sediment.
30300120	8	15	theme	cellular	874:881	arg1	profile					894:900	The total cellular fatty acid profile	864:900	The total cellular fatty acid profile	864:900	The total cellular fatty acid profile was dominated by C16 : 0 12-methyl.
30300120	14	16	theme	=DSM	1411:1414	arg1	1.16398T					1430:1437	=DSM 105148T=CGMCC 1.16398T	1411:1437	=DSM 105148T=CGMCC 1.16398T	1411:1437	nov., is proposed, with the type strain SCSIO 08198T (=DSM 105148T=CGMCC 1.16398T).
30300120	14	16	theme	=DSM	1411:1414	arg1	08198T					1403:1408	the type strain SCSIO 08198T	1381:1408	the type strain SCSIO 08198T (=DSM 105148T=CGMCC 1.16398T)	1381:1438	nov., is proposed, with the type strain SCSIO 08198T (=DSM 105148T=CGMCC 1.16398T).
30300120	9	17	theme	main	951:954	arg1	menaquinone					956:966	the main menaquinone	947:966	the main menaquinone	947:966	MK-8 was the main menaquinone.
30300120	9	17	theme	main	951:954	arg1	MK-8					938:941	MK-8	938:941	MK-8	938:941	MK-8 was the main menaquinone.
30300120	8	18	theme	C16 	919:922	arg1	12-methyl					927:935	C16 : 0 12-methyl	919:935	C16 : 0 12-methyl	919:935	The total cellular fatty acid profile was dominated by C16 : 0 12-methyl.
30300120	11	19	theme	unidentified	1093:1104	arg1	phospholipids					1106:1118	unidentified phospholipids	1093:1118	unidentified phospholipids	1093:1118	The major phospholipids were diphosphatidylglycerol, phosphatidylglycerol and unidentified phospholipids.
30300120	7	20	theme	novel	794:798	arg1	isolate					800:806	the novel isolate	790:806	the novel isolate	790:806	Phylogenetic analysis based on nearly complete 16S rRNA gene sequences revealed that the novel isolate shared a lineage with members of the genus Rubrobacter.
30300120	12	21	theme	mol	1225:1227	arg1	%					1228:1228	63.8 mol%	1220:1228	63.8 mol%	1220:1228	Based on the whole genome sequence analysis, the genome size is 3 078 689 bp with DNA G+C value of 63.8 mol%, including one circular chromosome and two plasmids.
30300120	14	22	theme	SCSIO	1397:1401	arg1	1.16398T					1430:1437	=DSM 105148T=CGMCC 1.16398T	1411:1437	=DSM 105148T=CGMCC 1.16398T	1411:1437	nov., is proposed, with the type strain SCSIO 08198T (=DSM 105148T=CGMCC 1.16398T).
30300120	14	22	theme	SCSIO	1397:1401	arg1	08198T					1403:1408	the type strain SCSIO 08198T	1381:1408	the type strain SCSIO 08198T (=DSM 105148T=CGMCC 1.16398T)	1381:1438	nov., is proposed, with the type strain SCSIO 08198T (=DSM 105148T=CGMCC 1.16398T).
30300120	4	23	theme	marine	354:359	arg1	agar					361:364	marine agar	354:364	marine agar with 1-5 % (w/v) NaCl and incubation at 28 °C for more than a fortnight	354:436	Good growth occurred on marine agar with 1-5 % (w/v) NaCl and incubation at 28 °C for more than a fortnight.
30300120	5	24	theme	short	452:456	arg1	radiation					470:478	short ultraviolet radiation	452:478	short ultraviolet radiation	452:478	Sensitive to short ultraviolet radiation.
30300120	6	25	theme	highest	559:565	arg1	similarity					567:576	the highest similarity	555:576	the highest similarity of 97.2 % to Rubrobacter radiotolerans DSM 5868T	555:625	Analysis of 16S rRNA gene sequences revealed that strain SCSIO 08198T had the highest similarity of 97.2 % to Rubrobacter radiotolerans DSM 5868T, and loosely related (<94.2 %) to all other species in the genus Rubrobacter.
30300120	2	26	theme	actinobacterial	126:140	arg1	strain					142:147	A novel mesophilic marine actinobacterial strain	100:147	A novel mesophilic marine actinobacterial strain	100:147	A novel mesophilic marine actinobacterial strain, designated as SCSIO 08198T, was isolated from a deep-sea sediment sample collected from the Indian Ocean.
30300120	12	27	theme	whole	1134:1138	arg1	analysis					1156:1163	the whole genome sequence analysis	1130:1163	the whole genome sequence analysis	1130:1163	Based on the whole genome sequence analysis, the genome size is 3 078 689 bp with DNA G+C value of 63.8 mol%, including one circular chromosome and two plasmids.
30300120	6	28	dep	related	640:646	arg1	%					655:655	<94.2 %	649:655	<94.2 %	649:655	Analysis of 16S rRNA gene sequences revealed that strain SCSIO 08198T had the highest similarity of 97.2 % to Rubrobacter radiotolerans DSM 5868T, and loosely related (<94.2 %) to all other species in the genus Rubrobacter.
30300120	1	29	theme	Indian	77:82	arg1	sediment					90:97	Indian Ocean sediment	77:97	Indian Ocean sediment	77:97	nov., a new marine actinobacterium isolated from Indian Ocean sediment.
30300120	5	30	theme	ultraviolet	458:468	arg1	radiation					470:478	short ultraviolet radiation	452:478	short ultraviolet radiation	452:478	Sensitive to short ultraviolet radiation.
30300120	0	31	theme	Rubrobacter	0:10	arg1	indicoceani					12:22	Rubrobacter indicoceani	0:22	Rubrobacter indicoceani	0:22	Rubrobacter indicoceani sp.
30300120	12	32	theme	genome	1170:1175	arg1	size					1177:1180	the genome size	1166:1180	the genome size	1166:1180	Based on the whole genome sequence analysis, the genome size is 3 078 689 bp with DNA G+C value of 63.8 mol%, including one circular chromosome and two plasmids.
30300120	12	32	theme	genome	1170:1175	arg1	3 078 689 bp					1185:1196	3 078 689 bp	1185:1196	3 078 689 bp	1185:1196	Based on the whole genome sequence analysis, the genome size is 3 078 689 bp with DNA G+C value of 63.8 mol%, including one circular chromosome and two plasmids.
30300120	7	33	theme	genus	845:849	arg1	Rubrobacter					851:861	the genus Rubrobacter	841:861	the genus Rubrobacter	841:861	Phylogenetic analysis based on nearly complete 16S rRNA gene sequences revealed that the novel isolate shared a lineage with members of the genus Rubrobacter.
30300120	1	34	theme	Ocean	84:88	arg1	sediment					90:97	Indian Ocean sediment	77:97	Indian Ocean sediment	77:97	nov., a new marine actinobacterium isolated from Indian Ocean sediment.
30300120	7	35	theme	gene	761:764	arg1	sequences					766:774	nearly complete 16S rRNA gene sequences	736:774	nearly complete 16S rRNA gene sequences	736:774	Phylogenetic analysis based on nearly complete 16S rRNA gene sequences revealed that the novel isolate shared a lineage with members of the genus Rubrobacter.
30300120	2	36	attach	isolated	182:189	arg1	sample					216:221	a deep-sea sediment sample	196:221	a deep-sea sediment sample collected from the Indian Ocean	196:253	A novel mesophilic marine actinobacterial strain, designated as SCSIO 08198T, was isolated from a deep-sea sediment sample collected from the Indian Ocean.
30300120	2	36	attach	isolated	182:189	arg2	strain					142:147	A novel mesophilic marine actinobacterial strain	100:147	A novel mesophilic marine actinobacterial strain	100:147	A novel mesophilic marine actinobacterial strain, designated as SCSIO 08198T, was isolated from a deep-sea sediment sample collected from the Indian Ocean.
30300120	7	37	theme	complete	743:750	arg1	sequences					766:774	nearly complete 16S rRNA gene sequences	736:774	nearly complete 16S rRNA gene sequences	736:774	Phylogenetic analysis based on nearly complete 16S rRNA gene sequences revealed that the novel isolate shared a lineage with members of the genus Rubrobacter.
30300120	7	38	theme	Rubrobacter	851:861	arg1	members					830:836	members	830:836	members of the genus Rubrobacter	830:861	Phylogenetic analysis based on nearly complete 16S rRNA gene sequences revealed that the novel isolate shared a lineage with members of the genus Rubrobacter.
30300120	12	39	with	3 078 689 bp	1185:1196	arg1	G+C value					1207:1215	DNA G+C value	1203:1215	DNA G+C value	1203:1215	Based on the whole genome sequence analysis, the genome size is 3 078 689 bp with DNA G+C value of 63.8 mol%, including one circular chromosome and two plasmids.
30300120	12	40	theme	DNA	1203:1205	arg1	G+C value					1207:1215	DNA G+C value	1203:1215	DNA G+C value	1203:1215	Based on the whole genome sequence analysis, the genome size is 3 078 689 bp with DNA G+C value of 63.8 mol%, including one circular chromosome and two plasmids.
30300120	13	41	dep	sp	1353:1354	arg1	species					1321:1327	a new species	1315:1327	a new species	1315:1327	Based on these polyphasic data, a new species, Rubrobacterindicoceani sp.
30300120	12	42	theme	genome	1140:1145	arg1	analysis					1156:1163	the whole genome sequence analysis	1130:1163	the whole genome sequence analysis	1130:1163	Based on the whole genome sequence analysis, the genome size is 3 078 689 bp with DNA G+C value of 63.8 mol%, including one circular chromosome and two plasmids.
30300120	7	43	theme	16S	752:754	arg1	sequences					766:774	nearly complete 16S rRNA gene sequences	736:774	nearly complete 16S rRNA gene sequences	736:774	Phylogenetic analysis based on nearly complete 16S rRNA gene sequences revealed that the novel isolate shared a lineage with members of the genus Rubrobacter.
30300120	6	44	theme	SCSIO	538:542	arg1	08198T					544:549	strain SCSIO 08198T	531:549	strain SCSIO 08198T	531:549	Analysis of 16S rRNA gene sequences revealed that strain SCSIO 08198T had the highest similarity of 97.2 % to Rubrobacter radiotolerans DSM 5868T, and loosely related (<94.2 %) to all other species in the genus Rubrobacter.
30300120	1	45	attach	isolated	63:70	arg1	sediment					90:97	Indian Ocean sediment	77:97	Indian Ocean sediment	77:97	nov., a new marine actinobacterium isolated from Indian Ocean sediment.
30300120	1	45	attach	isolated	63:70	arg2	actinobacterium					47:61	a new marine actinobacterium	34:61	a new marine actinobacterium isolated from Indian Ocean sediment	34:97	nov., a new marine actinobacterium isolated from Indian Ocean sediment.
30300120	4	46	from	28 °C	406:410	arg1	incubation					392:401	incubation	392:401	incubation at 28 °C for more than a fortnight	392:436	Good growth occurred on marine agar with 1-5 % (w/v) NaCl and incubation at 28 °C for more than a fortnight.
30300120	4	46	from	28 °C	406:410	arg1	NaCl					383:386	1-5 % (w/v) NaCl	371:386	1-5 % (w/v) NaCl	371:386	Good growth occurred on marine agar with 1-5 % (w/v) NaCl and incubation at 28 °C for more than a fortnight.
30300120	7	47	theme	rRNA	756:759	arg1	sequences					766:774	nearly complete 16S rRNA gene sequences	736:774	nearly complete 16S rRNA gene sequences	736:774	Phylogenetic analysis based on nearly complete 16S rRNA gene sequences revealed that the novel isolate shared a lineage with members of the genus Rubrobacter.
30300120	4	48	with	agar	361:364	arg1	incubation					392:401	incubation	392:401	incubation at 28 °C for more than a fortnight	392:436	Good growth occurred on marine agar with 1-5 % (w/v) NaCl and incubation at 28 °C for more than a fortnight.
30300120	4	48	with	agar	361:364	arg1	NaCl					383:386	1-5 % (w/v) NaCl	371:386	1-5 % (w/v) NaCl	371:386	Good growth occurred on marine agar with 1-5 % (w/v) NaCl and incubation at 28 °C for more than a fortnight.
30300120	7	49	theme	Phylogenetic	705:716	arg1	analysis					718:725	Phylogenetic analysis	705:725	Phylogenetic analysis based on nearly complete 16S rRNA gene sequences	705:774	Phylogenetic analysis based on nearly complete 16S rRNA gene sequences revealed that the novel isolate shared a lineage with members of the genus Rubrobacter.
30300120	6	50	theme	strain	531:536	arg1	08198T					544:549	strain SCSIO 08198T	531:549	strain SCSIO 08198T	531:549	Analysis of 16S rRNA gene sequences revealed that strain SCSIO 08198T had the highest similarity of 97.2 % to Rubrobacter radiotolerans DSM 5868T, and loosely related (<94.2 %) to all other species in the genus Rubrobacter.
30300120	6	51	theme	genus	686:690	arg1	Rubrobacter					692:702	the genus Rubrobacter	682:702	the genus Rubrobacter	682:702	Analysis of 16S rRNA gene sequences revealed that strain SCSIO 08198T had the highest similarity of 97.2 % to Rubrobacter radiotolerans DSM 5868T, and loosely related (<94.2 %) to all other species in the genus Rubrobacter.
30300120	4	52	theme	Good	330:333	arg1	growth					335:340	Good growth	330:340	Good growth	330:340	Good growth occurred on marine agar with 1-5 % (w/v) NaCl and incubation at 28 °C for more than a fortnight.
30300120	13	53	theme	polyphasic	1298:1307	arg1	data					1309:1312	these polyphasic data	1292:1312	these polyphasic data	1292:1312	Based on these polyphasic data, a new species, Rubrobacterindicoceani sp.
30300120	2	54	theme	sediment	207:214	arg1	sample					216:221	a deep-sea sediment sample	196:221	a deep-sea sediment sample collected from the Indian Ocean	196:253	A novel mesophilic marine actinobacterial strain, designated as SCSIO 08198T, was isolated from a deep-sea sediment sample collected from the Indian Ocean.
30300120	6	55	contain	had	551:553	arg2	similarity					567:576	the highest similarity	555:576	the highest similarity of 97.2 % to Rubrobacter radiotolerans DSM 5868T	555:625	Analysis of 16S rRNA gene sequences revealed that strain SCSIO 08198T had the highest similarity of 97.2 % to Rubrobacter radiotolerans DSM 5868T, and loosely related (<94.2 %) to all other species in the genus Rubrobacter.
30300120	6	55	contain	had	551:553	arg1	08198T					544:549	strain SCSIO 08198T	531:549	strain SCSIO 08198T	531:549	Analysis of 16S rRNA gene sequences revealed that strain SCSIO 08198T had the highest similarity of 97.2 % to Rubrobacter radiotolerans DSM 5868T, and loosely related (<94.2 %) to all other species in the genus Rubrobacter.
30300120	10	56	theme	peptidoglycan	973:985	arg1	A3α					996:998	A3α	996:998	A3α (l-Lys-l-Ala)	996:1012	The peptidoglycan type was A3α (l-Lys-l-Ala).
30300120	10	56	theme	peptidoglycan	973:985	arg1	type					987:990	The peptidoglycan type	969:990	The peptidoglycan type	969:990	The peptidoglycan type was A3α (l-Lys-l-Ala).
30300120	12	57	theme	%	1228:1228	arg1	G+C value					1207:1215	DNA G+C value	1203:1215	DNA G+C value	1203:1215	Based on the whole genome sequence analysis, the genome size is 3 078 689 bp with DNA G+C value of 63.8 mol%, including one circular chromosome and two plasmids.
30300120	14	58	theme	strain	1390:1395	arg1	1.16398T					1430:1437	=DSM 105148T=CGMCC 1.16398T	1411:1437	=DSM 105148T=CGMCC 1.16398T	1411:1437	nov., is proposed, with the type strain SCSIO 08198T (=DSM 105148T=CGMCC 1.16398T).
30300120	14	58	theme	strain	1390:1395	arg1	08198T					1403:1408	the type strain SCSIO 08198T	1381:1408	the type strain SCSIO 08198T (=DSM 105148T=CGMCC 1.16398T)	1381:1438	nov., is proposed, with the type strain SCSIO 08198T (=DSM 105148T=CGMCC 1.16398T).
30300120	2	59	theme	deep-sea	198:205	arg1	sample					216:221	a deep-sea sediment sample	196:221	a deep-sea sediment sample collected from the Indian Ocean	196:253	A novel mesophilic marine actinobacterial strain, designated as SCSIO 08198T, was isolated from a deep-sea sediment sample collected from the Indian Ocean.
30300120	6	60	from	species	671:677	arg1	Rubrobacter					692:702	the genus Rubrobacter	682:702	the genus Rubrobacter	682:702	Analysis of 16S rRNA gene sequences revealed that strain SCSIO 08198T had the highest similarity of 97.2 % to Rubrobacter radiotolerans DSM 5868T, and loosely related (<94.2 %) to all other species in the genus Rubrobacter.
30300120	8	61	theme	 0	924:925	arg1	12-methyl					927:935	C16 : 0 12-methyl	919:935	C16 : 0 12-methyl	919:935	The total cellular fatty acid profile was dominated by C16 : 0 12-methyl.
30300120	3	62	from	pink	314:317	arg1	colour					322:327	colour	322:327	colour	322:327	The strain was Gram-stain-positive, rod-shaped and salmon pink in colour.
30300120	6	63	theme	radiotolerans	603:615	arg1	DSM					617:619	Rubrobacter radiotolerans DSM 5868T	591:625	Rubrobacter radiotolerans DSM 5868T	591:625	Analysis of 16S rRNA gene sequences revealed that strain SCSIO 08198T had the highest similarity of 97.2 % to Rubrobacter radiotolerans DSM 5868T, and loosely related (<94.2 %) to all other species in the genus Rubrobacter.
30300120	0	64	dep	sp	24:25	arg1	indicoceani					12:22	Rubrobacter indicoceani	0:22	Rubrobacter indicoceani	0:22	Rubrobacter indicoceani sp.
30300120	6	65	theme	other	665:669	arg1	species					671:677	all other species	661:677	all other species in the genus Rubrobacter	661:702	Analysis of 16S rRNA gene sequences revealed that strain SCSIO 08198T had the highest similarity of 97.2 % to Rubrobacter radiotolerans DSM 5868T, and loosely related (<94.2 %) to all other species in the genus Rubrobacter.
30300120	1	66	dep	actinobacterium	47:61	arg1	nov.					28:31	nov.	28:31	nov.	28:31	nov., a new marine actinobacterium isolated from Indian Ocean sediment.
30300120	6	67	theme	Rubrobacter	591:601	arg1	DSM					617:619	Rubrobacter radiotolerans DSM 5868T	591:625	Rubrobacter radiotolerans DSM 5868T	591:625	Analysis of 16S rRNA gene sequences revealed that strain SCSIO 08198T had the highest similarity of 97.2 % to Rubrobacter radiotolerans DSM 5868T, and loosely related (<94.2 %) to all other species in the genus Rubrobacter.
30300120	6	68	theme	sequences	507:515	arg1	Analysis					481:488	Analysis	481:488	Analysis of 16S rRNA gene sequences	481:515	Analysis of 16S rRNA gene sequences revealed that strain SCSIO 08198T had the highest similarity of 97.2 % to Rubrobacter radiotolerans DSM 5868T, and loosely related (<94.2 %) to all other species in the genus Rubrobacter.
30300120	3	69	from	colour	322:327	arg1	pink					314:317	pink	314:317	pink	314:317	The strain was Gram-stain-positive, rod-shaped and salmon pink in colour.
30300120	8	70	theme	total	868:872	arg1	profile					894:900	The total cellular fatty acid profile	864:900	The total cellular fatty acid profile	864:900	The total cellular fatty acid profile was dominated by C16 : 0 12-methyl.
30300120	14	71	theme	type	1385:1388	arg1	1.16398T					1430:1437	=DSM 105148T=CGMCC 1.16398T	1411:1437	=DSM 105148T=CGMCC 1.16398T	1411:1437	nov., is proposed, with the type strain SCSIO 08198T (=DSM 105148T=CGMCC 1.16398T).
30300120	14	71	theme	type	1385:1388	arg1	08198T					1403:1408	the type strain SCSIO 08198T	1381:1408	the type strain SCSIO 08198T (=DSM 105148T=CGMCC 1.16398T)	1381:1438	nov., is proposed, with the type strain SCSIO 08198T (=DSM 105148T=CGMCC 1.16398T).
30300120	6	72	theme	gene	502:505	arg1	sequences					507:515	16S rRNA gene sequences	493:515	16S rRNA gene sequences	493:515	Analysis of 16S rRNA gene sequences revealed that strain SCSIO 08198T had the highest similarity of 97.2 % to Rubrobacter radiotolerans DSM 5868T, and loosely related (<94.2 %) to all other species in the genus Rubrobacter.
30300120	2	73	theme	marine	119:124	arg1	strain					142:147	A novel mesophilic marine actinobacterial strain	100:147	A novel mesophilic marine actinobacterial strain	100:147	A novel mesophilic marine actinobacterial strain, designated as SCSIO 08198T, was isolated from a deep-sea sediment sample collected from the Indian Ocean.
30300120	1	74	theme	new	36:38	arg1	actinobacterium					47:61	a new marine actinobacterium	34:61	a new marine actinobacterium isolated from Indian Ocean sediment	34:97	nov., a new marine actinobacterium isolated from Indian Ocean sediment.
30300120	8	75	theme	acid	889:892	arg1	profile					894:900	The total cellular fatty acid profile	864:900	The total cellular fatty acid profile	864:900	The total cellular fatty acid profile was dominated by C16 : 0 12-methyl.
30300120	12	76	theme	sequence	1147:1154	arg1	analysis					1156:1163	the whole genome sequence analysis	1130:1163	the whole genome sequence analysis	1130:1163	Based on the whole genome sequence analysis, the genome size is 3 078 689 bp with DNA G+C value of 63.8 mol%, including one circular chromosome and two plasmids.
30300120	6	77	theme	%	586:586	arg1	similarity					567:576	the highest similarity	555:576	the highest similarity of 97.2 % to Rubrobacter radiotolerans DSM 5868T	555:625	Analysis of 16S rRNA gene sequences revealed that strain SCSIO 08198T had the highest similarity of 97.2 % to Rubrobacter radiotolerans DSM 5868T, and loosely related (<94.2 %) to all other species in the genus Rubrobacter.
33192120	0	0	from	Panax	34:38	arg1	oligosaccharides					12:27	oligosaccharides	12:27	oligosaccharides from Panax	12:38	Analysis of oligosaccharides from Panax ginseng by using solid-phase permethylation method combined with ultra-high-performance liquid chromatography-Q-Orbitrap/mass spectrometry.
33192120	0	0	from	Panax	34:38	arg1	Analysis					0:7	Analysis	0:7	Analysis of oligosaccharides from Panax	0:38	Analysis of oligosaccharides from Panax ginseng by using solid-phase permethylation method combined with ultra-high-performance liquid chromatography-Q-Orbitrap/mass spectrometry.
33192120	9	1	theme	large	1352:1356	arg1	projection value					1335:1350	the projection value	1331:1350	the projection value large than 1.0	1331:1365	The glycans with variable importance in the projection value large than 1.0 were considered to contribute most to the classification.
33192120	9	2	with	glycans	1295:1301	arg1	importance					1317:1326	variable importance	1308:1326	variable importance	1308:1326	The glycans with variable importance in the projection value large than 1.0 were considered to contribute most to the classification.
33192120	11	3	theme	solid-phase	1726:1736	arg1	method					1750:1755	A solid-phase methylation method	1724:1755	A solid-phase methylation method combined with liquid chromatography-tandem mass spectrometry	1724:1816	CONCLUSION A solid-phase methylation method combined with liquid chromatography-tandem mass spectrometry was successfully applied to analyze the oligosaccharides in ginseng extracts, which provides the possibility for holistic evaluation of ginseng oligosaccharides.
33192120	10	4	from	contents	1429:1436	arg1	ginseng					1465:1471	red ginseng	1461:1471	red ginseng	1461:1471	The contents of oligosaccharides in red ginseng were lower than those in white ginseng, and the contents of maltose, maltotriose, maltotetraose, maltopentaose, maltohexaose, maltoheptaose, maltooctaose, maltononaose, sucrose, and erlose decreased significantly (p < 0.05) in red ginseng.
33192120	8	5	theme	least	1171:1175	arg1	analysis					1198:1205	the partial least squares-discriminate analysis	1159:1205	the partial least squares-discriminate analysis score plot	1159:1216	In the comparison of the oligosaccharide extracts from white ginseng and red ginseng, a clear separation was observed in the partial least squares-discriminate analysis score plot, indicating the sugar differences in these two kinds of ginseng samples.
33192120	10	6	from	oligosaccharides	1441:1456	arg1	ginseng					1465:1471	red ginseng	1461:1471	red ginseng	1461:1471	The contents of oligosaccharides in red ginseng were lower than those in white ginseng, and the contents of maltose, maltotriose, maltotetraose, maltopentaose, maltohexaose, maltoheptaose, maltooctaose, maltononaose, sucrose, and erlose decreased significantly (p < 0.05) in red ginseng.
33192120	2	7	theme	ginseng	344:350	arg1	oligosaccharides					352:367	ginseng oligosaccharides	344:367	ginseng oligosaccharides	344:367	There is an urgent need to develop a practical procedure to detect and analyze ginseng oligosaccharides.
33192120	12	8	from	differences	2085:2095	arg1	activities					2116:2125	pharmacological activities	2100:2125	pharmacological activities	2100:2125	The comparison of oligosaccharide composition of white ginseng and red ginseng could help understand the differences in pharmacological activities between these two kinds of ginseng samples from the perspective of glycans.
33192120	12	9	theme	glycans	2194:2200	arg1	perspective					2179:2189	the perspective	2175:2189	the perspective of glycans	2175:2200	The comparison of oligosaccharide composition of white ginseng and red ginseng could help understand the differences in pharmacological activities between these two kinds of ginseng samples from the perspective of glycans.
33192120	6	10	from	composition	802:812	arg1	ginseng					823:829	ginseng	823:829	ginseng	823:829	The oligosaccharide composition in white ginseng and red ginseng was compared using a multivariate statistical analysis method.
33192120	6	10	from	composition	802:812	arg1	ginseng					839:845	ginseng	839:845	ginseng	839:845	The oligosaccharide composition in white ginseng and red ginseng was compared using a multivariate statistical analysis method.
33192120	6	11	theme	oligosaccharide	786:800	arg1	composition					802:812	The oligosaccharide composition	782:812	The oligosaccharide composition in white ginseng and red ginseng	782:845	The oligosaccharide composition in white ginseng and red ginseng was compared using a multivariate statistical analysis method.
33192120	11	12	theme	ginseng	1954:1960	arg1	oligosaccharides					1962:1977	ginseng oligosaccharides	1954:1977	ginseng oligosaccharides	1954:1977	CONCLUSION A solid-phase methylation method combined with liquid chromatography-tandem mass spectrometry was successfully applied to analyze the oligosaccharides in ginseng extracts, which provides the possibility for holistic evaluation of ginseng oligosaccharides.
33192120	3	13	from	extracts	398:405	arg1	METHODS					370:376	METHODS	370:376	METHODS The oligosaccharide extracts from ginseng	370:418	METHODS The oligosaccharide extracts from ginseng were permethylated by solid-phase methylation method and then were analyzed by ultra-high-performance liquid chromatography-Q-Orbitrap/MS.
33192120	2	14	theme	practical	302:310	arg1	procedure					312:320	a practical procedure	300:320	a practical procedure to detect and analyze ginseng oligosaccharides	300:367	There is an urgent need to develop a practical procedure to detect and analyze ginseng oligosaccharides.
33192120	8	15	dep	ginseng	1115:1121	arg1	red					1111:1113	red	1111:1113	red	1111:1113	In the comparison of the oligosaccharide extracts from white ginseng and red ginseng, a clear separation was observed in the partial least squares-discriminate analysis score plot, indicating the sugar differences in these two kinds of ginseng samples.
33192120	0	16	theme	solid-phase	57:67	arg1	method					84:89	solid-phase permethylation method	57:89	solid-phase permethylation method combined with ultra-high-performance liquid chromatography-Q-Orbitrap/mass spectrometry	57:177	Analysis of oligosaccharides from Panax ginseng by using solid-phase permethylation method combined with ultra-high-performance liquid chromatography-Q-Orbitrap/mass spectrometry.
33192120	8	17	theme	analysis	1198:1205	arg1	plot					1213:1216	the partial least squares-discriminate analysis score plot	1159:1216	the partial least squares-discriminate analysis score plot	1159:1216	In the comparison of the oligosaccharide extracts from white ginseng and red ginseng, a clear separation was observed in the partial least squares-discriminate analysis score plot, indicating the sugar differences in these two kinds of ginseng samples.
33192120	0	18	from	Analysis	0:7	arg1	Panax					34:38	Panax	34:38	Panax	34:38	Analysis of oligosaccharides from Panax ginseng by using solid-phase permethylation method combined with ultra-high-performance liquid chromatography-Q-Orbitrap/mass spectrometry.
33192120	1	19	dep	BACKGROUND	180:189	arg1	reports					195:201	The reports	191:201	The reports about valuable oligosaccharides in ginseng	191:244	BACKGROUND The reports about valuable oligosaccharides in ginseng are quite limited.
33192120	1	19	dep	BACKGROUND	180:189	arg1	limited					256:262	limited	256:262	limited	256:262	BACKGROUND The reports about valuable oligosaccharides in ginseng are quite limited.
33192120	12	20	theme	samples	2162:2168	arg1	kinds					2145:2149	these two kinds	2135:2149	these two kinds of ginseng samples from the perspective of glycans	2135:2200	The comparison of oligosaccharide composition of white ginseng and red ginseng could help understand the differences in pharmacological activities between these two kinds of ginseng samples from the perspective of glycans.
33192120	6	21	dep	ginseng	823:829	arg1	white					817:821	white	817:821	white	817:821	The oligosaccharide composition in white ginseng and red ginseng was compared using a multivariate statistical analysis method.
33192120	0	22	theme	liquid	128:133	arg1	spectrometry					166:177	ultra-high-performance liquid chromatography-Q-Orbitrap/mass spectrometry	105:177	ultra-high-performance liquid chromatography-Q-Orbitrap/mass spectrometry	105:177	Analysis of oligosaccharides from Panax ginseng by using solid-phase permethylation method combined with ultra-high-performance liquid chromatography-Q-Orbitrap/mass spectrometry.
33192120	4	23	theme	configuration	586:598	arg1	information					600:610	The sequence, linkage, and configuration information	559:610	The sequence, linkage, and configuration information of oligosaccharides	559:630	The sequence, linkage, and configuration information of oligosaccharides were determined by using accurate m/z value and tandem mass information.
33192120	3	24	theme	ultra-high-performance	499:520	arg1	chromatography-Q-Orbitrap/MS					529:556	ultra-high-performance liquid chromatography-Q-Orbitrap/MS	499:556	ultra-high-performance liquid chromatography-Q-Orbitrap/MS	499:556	METHODS The oligosaccharide extracts from ginseng were permethylated by solid-phase methylation method and then were analyzed by ultra-high-performance liquid chromatography-Q-Orbitrap/MS.
33192120	7	25	theme	oligosaccharides	966:981	arg1	identified was					983:996	12 oligosaccharides identified was	963:996	12 oligosaccharides identified was reported for the first time in ginseng	963:1035	RESULTS The nonreducing oligosaccharide erlose among 12 oligosaccharides identified was reported for the first time in ginseng.
33192120	8	26	theme	sugar	1234:1238	arg1	differences					1240:1250	the sugar differences	1230:1250	the sugar differences in these two kinds of ginseng samples	1230:1288	In the comparison of the oligosaccharide extracts from white ginseng and red ginseng, a clear separation was observed in the partial least squares-discriminate analysis score plot, indicating the sugar differences in these two kinds of ginseng samples.
33192120	5	27	theme	Several	705:711	arg1	references					722:731	Several standard references	705:731	Several standard references	705:731	Several standard references were used to further confirm the identification.
33192120	8	28	theme	oligosaccharide	1063:1077	arg1	extracts					1079:1086	the oligosaccharide extracts	1059:1086	the oligosaccharide extracts from white ginseng and red ginseng	1059:1121	In the comparison of the oligosaccharide extracts from white ginseng and red ginseng, a clear separation was observed in the partial least squares-discriminate analysis score plot, indicating the sugar differences in these two kinds of ginseng samples.
33192120	3	29	theme	solid-phase	442:452	arg1	method					466:471	solid-phase methylation method	442:471	solid-phase methylation method	442:471	METHODS The oligosaccharide extracts from ginseng were permethylated by solid-phase methylation method and then were analyzed by ultra-high-performance liquid chromatography-Q-Orbitrap/MS.
33192120	0	30	theme	oligosaccharides	12:27	arg1	Analysis					0:7	Analysis	0:7	Analysis of oligosaccharides from Panax	0:38	Analysis of oligosaccharides from Panax ginseng by using solid-phase permethylation method combined with ultra-high-performance liquid chromatography-Q-Orbitrap/mass spectrometry.
33192120	4	31	theme	tandem	680:685	arg1	information					692:702	tandem mass information	680:702	tandem mass information	680:702	The sequence, linkage, and configuration information of oligosaccharides were determined by using accurate m/z value and tandem mass information.
33192120	3	32	dep	extracts	398:405	arg1	ginseng					412:418	ginseng	412:418	ginseng	412:418	METHODS The oligosaccharide extracts from ginseng were permethylated by solid-phase methylation method and then were analyzed by ultra-high-performance liquid chromatography-Q-Orbitrap/MS.
33192120	7	33	theme	oligosaccharide	934:948	arg1	erlose					950:955	The nonreducing oligosaccharide erlose	918:955	The nonreducing oligosaccharide erlose among 12 oligosaccharides identified was reported for the first time in ginseng	918:1035	RESULTS The nonreducing oligosaccharide erlose among 12 oligosaccharides identified was reported for the first time in ginseng.
33192120	12	34	theme	pharmacological	2100:2114	arg1	activities					2116:2125	pharmacological activities	2100:2125	pharmacological activities	2100:2125	The comparison of oligosaccharide composition of white ginseng and red ginseng could help understand the differences in pharmacological activities between these two kinds of ginseng samples from the perspective of glycans.
33192120	8	35	from	ginseng	1115:1121	arg1	extracts					1079:1086	the oligosaccharide extracts	1059:1086	the oligosaccharide extracts from white ginseng and red ginseng	1059:1121	In the comparison of the oligosaccharide extracts from white ginseng and red ginseng, a clear separation was observed in the partial least squares-discriminate analysis score plot, indicating the sugar differences in these two kinds of ginseng samples.
33192120	11	36	theme	liquid	1771:1776	arg1	spectrometry					1805:1816	liquid chromatography-tandem mass spectrometry	1771:1816	liquid chromatography-tandem mass spectrometry	1771:1816	CONCLUSION A solid-phase methylation method combined with liquid chromatography-tandem mass spectrometry was successfully applied to analyze the oligosaccharides in ginseng extracts, which provides the possibility for holistic evaluation of ginseng oligosaccharides.
33192120	12	37	theme	ginseng	2051:2057	arg1	composition					2014:2024	oligosaccharide composition	1998:2024	oligosaccharide composition of white ginseng and red ginseng	1998:2057	The comparison of oligosaccharide composition of white ginseng and red ginseng could help understand the differences in pharmacological activities between these two kinds of ginseng samples from the perspective of glycans.
33192120	4	38	theme	accurate	657:664	arg1	value					670:674	accurate m/z value	657:674	accurate m/z value	657:674	The sequence, linkage, and configuration information of oligosaccharides were determined by using accurate m/z value and tandem mass information.
33192120	11	39	theme	holistic	1931:1938	arg1	evaluation					1940:1949	holistic evaluation	1931:1949	holistic evaluation of ginseng oligosaccharides	1931:1977	CONCLUSION A solid-phase methylation method combined with liquid chromatography-tandem mass spectrometry was successfully applied to analyze the oligosaccharides in ginseng extracts, which provides the possibility for holistic evaluation of ginseng oligosaccharides.
33192120	7	40	from	time	1021:1024	arg1	ginseng					1029:1035	ginseng	1029:1035	ginseng	1029:1035	RESULTS The nonreducing oligosaccharide erlose among 12 oligosaccharides identified was reported for the first time in ginseng.
33192120	9	41	theme	variable	1308:1315	arg1	importance					1317:1326	variable importance	1308:1326	variable importance	1308:1326	The glycans with variable importance in the projection value large than 1.0 were considered to contribute most to the classification.
33192120	8	42	theme	samples	1282:1288	arg1	kinds					1265:1269	these two kinds	1255:1269	these two kinds of ginseng samples	1255:1288	In the comparison of the oligosaccharide extracts from white ginseng and red ginseng, a clear separation was observed in the partial least squares-discriminate analysis score plot, indicating the sugar differences in these two kinds of ginseng samples.
33192120	12	43	from	perspective	2179:2189	arg1	kinds					2145:2149	these two kinds	2135:2149	these two kinds of ginseng samples from the perspective of glycans	2135:2200	The comparison of oligosaccharide composition of white ginseng and red ginseng could help understand the differences in pharmacological activities between these two kinds of ginseng samples from the perspective of glycans.
33192120	12	43	from	perspective	2179:2189	arg1	samples					2162:2168	ginseng samples	2154:2168	ginseng samples from the perspective of glycans	2154:2200	The comparison of oligosaccharide composition of white ginseng and red ginseng could help understand the differences in pharmacological activities between these two kinds of ginseng samples from the perspective of glycans.
33192120	12	44	theme	composition	2014:2024	arg1	comparison					1984:1993	The comparison	1980:1993	The comparison of oligosaccharide composition of white ginseng and red ginseng	1980:2057	The comparison of oligosaccharide composition of white ginseng and red ginseng could help understand the differences in pharmacological activities between these two kinds of ginseng samples from the perspective of glycans.
33192120	6	45	theme	multivariate	868:879	arg1	method					902:907	a multivariate statistical analysis method	866:907	a multivariate statistical analysis method	866:907	The oligosaccharide composition in white ginseng and red ginseng was compared using a multivariate statistical analysis method.
33192120	8	46	theme	clear	1126:1130	arg1	separation					1132:1141	a clear separation	1124:1141	a clear separation	1124:1141	In the comparison of the oligosaccharide extracts from white ginseng and red ginseng, a clear separation was observed in the partial least squares-discriminate analysis score plot, indicating the sugar differences in these two kinds of ginseng samples.
33192120	11	47	dep	CONCLUSION	1713:1722	arg1	applied					1835:1841	applied	1835:1841	applied to analyze the oligosaccharides in ginseng extracts	1835:1893	CONCLUSION A solid-phase methylation method combined with liquid chromatography-tandem mass spectrometry was successfully applied to analyze the oligosaccharides in ginseng extracts, which provides the possibility for holistic evaluation of ginseng oligosaccharides.
33192120	10	48	theme	red	1700:1702	arg1	ginseng					1704:1710	red ginseng	1700:1710	red ginseng	1700:1710	The contents of oligosaccharides in red ginseng were lower than those in white ginseng, and the contents of maltose, maltotriose, maltotetraose, maltopentaose, maltohexaose, maltoheptaose, maltooctaose, maltononaose, sucrose, and erlose decreased significantly (p < 0.05) in red ginseng.
33192120	11	49	theme	mass	1800:1803	arg1	spectrometry					1805:1816	liquid chromatography-tandem mass spectrometry	1771:1816	liquid chromatography-tandem mass spectrometry	1771:1816	CONCLUSION A solid-phase methylation method combined with liquid chromatography-tandem mass spectrometry was successfully applied to analyze the oligosaccharides in ginseng extracts, which provides the possibility for holistic evaluation of ginseng oligosaccharides.
33192120	12	50	theme	ginseng	2154:2160	arg1	samples					2162:2168	ginseng samples	2154:2168	ginseng samples from the perspective of glycans	2154:2200	The comparison of oligosaccharide composition of white ginseng and red ginseng could help understand the differences in pharmacological activities between these two kinds of ginseng samples from the perspective of glycans.
33192120	8	51	dep	extracts	1079:1086	arg1	comparison					1045:1054	comparison	1045:1054	comparison	1045:1054	In the comparison of the oligosaccharide extracts from white ginseng and red ginseng, a clear separation was observed in the partial least squares-discriminate analysis score plot, indicating the sugar differences in these two kinds of ginseng samples.
33192120	8	51	dep	extracts	1079:1086	arg1	the					1041:1043	the	1041:1043	the	1041:1043	In the comparison of the oligosaccharide extracts from white ginseng and red ginseng, a clear separation was observed in the partial least squares-discriminate analysis score plot, indicating the sugar differences in these two kinds of ginseng samples.
33192120	11	52	theme	ginseng	1878:1884	arg1	extracts					1886:1893	ginseng extracts	1878:1893	ginseng extracts	1878:1893	CONCLUSION A solid-phase methylation method combined with liquid chromatography-tandem mass spectrometry was successfully applied to analyze the oligosaccharides in ginseng extracts, which provides the possibility for holistic evaluation of ginseng oligosaccharides.
33192120	8	53	theme	partial	1163:1169	arg1	analysis					1198:1205	the partial least squares-discriminate analysis	1159:1205	the partial least squares-discriminate analysis score plot	1159:1216	In the comparison of the oligosaccharide extracts from white ginseng and red ginseng, a clear separation was observed in the partial least squares-discriminate analysis score plot, indicating the sugar differences in these two kinds of ginseng samples.
33192120	11	54	theme	methylation	1738:1748	arg1	method					1750:1755	A solid-phase methylation method	1724:1755	A solid-phase methylation method combined with liquid chromatography-tandem mass spectrometry	1724:1816	CONCLUSION A solid-phase methylation method combined with liquid chromatography-tandem mass spectrometry was successfully applied to analyze the oligosaccharides in ginseng extracts, which provides the possibility for holistic evaluation of ginseng oligosaccharides.
33192120	8	55	theme	squares-discriminate	1177:1196	arg1	analysis					1198:1205	the partial least squares-discriminate analysis	1159:1205	the partial least squares-discriminate analysis score plot	1159:1216	In the comparison of the oligosaccharide extracts from white ginseng and red ginseng, a clear separation was observed in the partial least squares-discriminate analysis score plot, indicating the sugar differences in these two kinds of ginseng samples.
33192120	7	56	theme	first	1015:1019	arg1	time					1021:1024	the first time	1011:1024	the first time in ginseng	1011:1035	RESULTS The nonreducing oligosaccharide erlose among 12 oligosaccharides identified was reported for the first time in ginseng.
33192120	4	57	theme	sequence	563:570	arg1	information					600:610	The sequence, linkage, and configuration information	559:610	The sequence, linkage, and configuration information of oligosaccharides	559:630	The sequence, linkage, and configuration information of oligosaccharides were determined by using accurate m/z value and tandem mass information.
33192120	8	58	from	differences	1240:1250	arg1	kinds					1265:1269	these two kinds	1255:1269	these two kinds of ginseng samples	1255:1288	In the comparison of the oligosaccharide extracts from white ginseng and red ginseng, a clear separation was observed in the partial least squares-discriminate analysis score plot, indicating the sugar differences in these two kinds of ginseng samples.
33192120	4	59	theme	linkage	573:579	arg1	information					600:610	The sequence, linkage, and configuration information	559:610	The sequence, linkage, and configuration information of oligosaccharides	559:630	The sequence, linkage, and configuration information of oligosaccharides were determined by using accurate m/z value and tandem mass information.
33192120	9	60	from	glycans	1295:1301	arg1	projection value					1335:1350	the projection value	1331:1350	the projection value large than 1.0	1331:1365	The glycans with variable importance in the projection value large than 1.0 were considered to contribute most to the classification.
33192120	2	61	theme	urgent	277:282	arg1	need					284:287	an urgent need	274:287	an urgent need to develop a practical procedure to detect and analyze ginseng oligosaccharides	274:367	There is an urgent need to develop a practical procedure to detect and analyze ginseng oligosaccharides.
33192120	10	62	theme	white	1498:1502	arg1	ginseng					1504:1510	white ginseng	1498:1510	white ginseng	1498:1510	The contents of oligosaccharides in red ginseng were lower than those in white ginseng, and the contents of maltose, maltotriose, maltotetraose, maltopentaose, maltohexaose, maltoheptaose, maltooctaose, maltononaose, sucrose, and erlose decreased significantly (p < 0.05) in red ginseng.
33192120	10	63	from	ginseng	1465:1471	arg1	contents					1429:1436	The contents	1425:1436	The contents of oligosaccharides in red ginseng	1425:1471	The contents of oligosaccharides in red ginseng were lower than those in white ginseng, and the contents of maltose, maltotriose, maltotetraose, maltopentaose, maltohexaose, maltoheptaose, maltooctaose, maltononaose, sucrose, and erlose decreased significantly (p < 0.05) in red ginseng.
33192120	10	63	from	ginseng	1465:1471	arg1	lower					1478:1482	lower	1478:1482	lower	1478:1482	The contents of oligosaccharides in red ginseng were lower than those in white ginseng, and the contents of maltose, maltotriose, maltotetraose, maltopentaose, maltohexaose, maltoheptaose, maltooctaose, maltononaose, sucrose, and erlose decreased significantly (p < 0.05) in red ginseng.
33192120	6	64	theme	analysis	893:900	arg1	method					902:907	a multivariate statistical analysis method	866:907	a multivariate statistical analysis method	866:907	The oligosaccharide composition in white ginseng and red ginseng was compared using a multivariate statistical analysis method.
33192120	11	65	theme	oligosaccharides	1962:1977	arg1	evaluation					1940:1949	holistic evaluation	1931:1949	holistic evaluation of ginseng oligosaccharides	1931:1977	CONCLUSION A solid-phase methylation method combined with liquid chromatography-tandem mass spectrometry was successfully applied to analyze the oligosaccharides in ginseng extracts, which provides the possibility for holistic evaluation of ginseng oligosaccharides.
33192120	0	66	theme	permethylation	69:82	arg1	method					84:89	solid-phase permethylation method	57:89	solid-phase permethylation method combined with ultra-high-performance liquid chromatography-Q-Orbitrap/mass spectrometry	57:177	Analysis of oligosaccharides from Panax ginseng by using solid-phase permethylation method combined with ultra-high-performance liquid chromatography-Q-Orbitrap/mass spectrometry.
33192120	4	67	theme	oligosaccharides	615:630	arg1	information					600:610	The sequence, linkage, and configuration information	559:610	The sequence, linkage, and configuration information of oligosaccharides	559:630	The sequence, linkage, and configuration information of oligosaccharides were determined by using accurate m/z value and tandem mass information.
33192120	10	68	theme	oligosaccharides	1441:1456	arg1	contents					1429:1436	The contents	1425:1436	The contents of oligosaccharides in red ginseng	1425:1471	The contents of oligosaccharides in red ginseng were lower than those in white ginseng, and the contents of maltose, maltotriose, maltotetraose, maltopentaose, maltohexaose, maltoheptaose, maltooctaose, maltononaose, sucrose, and erlose decreased significantly (p < 0.05) in red ginseng.
33192120	10	68	theme	oligosaccharides	1441:1456	arg1	lower					1478:1482	lower	1478:1482	lower	1478:1482	The contents of oligosaccharides in red ginseng were lower than those in white ginseng, and the contents of maltose, maltotriose, maltotetraose, maltopentaose, maltohexaose, maltoheptaose, maltooctaose, maltononaose, sucrose, and erlose decreased significantly (p < 0.05) in red ginseng.
33192120	8	69	theme	score	1207:1211	arg1	plot					1213:1216	the partial least squares-discriminate analysis score plot	1159:1216	the partial least squares-discriminate analysis score plot	1159:1216	In the comparison of the oligosaccharide extracts from white ginseng and red ginseng, a clear separation was observed in the partial least squares-discriminate analysis score plot, indicating the sugar differences in these two kinds of ginseng samples.
33192120	1	70	theme	valuable	209:216	arg1	oligosaccharides					218:233	valuable oligosaccharides	209:233	valuable oligosaccharides in ginseng	209:244	BACKGROUND The reports about valuable oligosaccharides in ginseng are quite limited.
33192120	12	71	from	kinds	2145:2149	arg1	perspective					2179:2189	the perspective	2175:2189	the perspective of glycans	2175:2200	The comparison of oligosaccharide composition of white ginseng and red ginseng could help understand the differences in pharmacological activities between these two kinds of ginseng samples from the perspective of glycans.
33192120	3	72	theme	oligosaccharide	382:396	arg1	extracts					398:405	The oligosaccharide extracts	378:405	The oligosaccharide extracts	378:405	METHODS The oligosaccharide extracts from ginseng were permethylated by solid-phase methylation method and then were analyzed by ultra-high-performance liquid chromatography-Q-Orbitrap/MS.
33192120	8	73	dep	ginseng	1099:1105	arg1	white					1093:1097	white	1093:1097	white	1093:1097	In the comparison of the oligosaccharide extracts from white ginseng and red ginseng, a clear separation was observed in the partial least squares-discriminate analysis score plot, indicating the sugar differences in these two kinds of ginseng samples.
33192120	0	74	theme	ultra-high-performance	105:126	arg1	spectrometry					166:177	ultra-high-performance liquid chromatography-Q-Orbitrap/mass spectrometry	105:177	ultra-high-performance liquid chromatography-Q-Orbitrap/mass spectrometry	105:177	Analysis of oligosaccharides from Panax ginseng by using solid-phase permethylation method combined with ultra-high-performance liquid chromatography-Q-Orbitrap/mass spectrometry.
33192120	1	75	from	oligosaccharides	218:233	arg1	ginseng					238:244	ginseng	238:244	ginseng	238:244	BACKGROUND The reports about valuable oligosaccharides in ginseng are quite limited.
33192120	6	76	theme	statistical	881:891	arg1	method					902:907	a multivariate statistical analysis method	866:907	a multivariate statistical analysis method	866:907	The oligosaccharide composition in white ginseng and red ginseng was compared using a multivariate statistical analysis method.
33192120	0	77	theme	chromatography-Q-Orbitrap/mass	135:164	arg1	spectrometry					166:177	ultra-high-performance liquid chromatography-Q-Orbitrap/mass spectrometry	105:177	ultra-high-performance liquid chromatography-Q-Orbitrap/mass spectrometry	105:177	Analysis of oligosaccharides from Panax ginseng by using solid-phase permethylation method combined with ultra-high-performance liquid chromatography-Q-Orbitrap/mass spectrometry.
33192120	6	78	dep	ginseng	839:845	arg1	red					835:837	red	835:837	red	835:837	The oligosaccharide composition in white ginseng and red ginseng was compared using a multivariate statistical analysis method.
33192120	10	79	theme	red	1461:1463	arg1	ginseng					1465:1471	red ginseng	1461:1471	red ginseng	1461:1471	The contents of oligosaccharides in red ginseng were lower than those in white ginseng, and the contents of maltose, maltotriose, maltotetraose, maltopentaose, maltohexaose, maltoheptaose, maltooctaose, maltononaose, sucrose, and erlose decreased significantly (p < 0.05) in red ginseng.
33192120	10	80	dep	decreased	1662:1670	arg1	p < 0.05					1687:1694	p < 0.05	1687:1694	p < 0.05	1687:1694	The contents of oligosaccharides in red ginseng were lower than those in white ginseng, and the contents of maltose, maltotriose, maltotetraose, maltopentaose, maltohexaose, maltoheptaose, maltooctaose, maltononaose, sucrose, and erlose decreased significantly (p < 0.05) in red ginseng.
33192120	8	81	from	ginseng	1099:1105	arg1	extracts					1079:1086	the oligosaccharide extracts	1059:1086	the oligosaccharide extracts from white ginseng and red ginseng	1059:1121	In the comparison of the oligosaccharide extracts from white ginseng and red ginseng, a clear separation was observed in the partial least squares-discriminate analysis score plot, indicating the sugar differences in these two kinds of ginseng samples.
33192120	11	82	from	oligosaccharides	1858:1873	arg1	extracts					1886:1893	ginseng extracts	1878:1893	ginseng extracts	1878:1893	CONCLUSION A solid-phase methylation method combined with liquid chromatography-tandem mass spectrometry was successfully applied to analyze the oligosaccharides in ginseng extracts, which provides the possibility for holistic evaluation of ginseng oligosaccharides.
33192120	3	83	theme	liquid	522:527	arg1	chromatography-Q-Orbitrap/MS					529:556	ultra-high-performance liquid chromatography-Q-Orbitrap/MS	499:556	ultra-high-performance liquid chromatography-Q-Orbitrap/MS	499:556	METHODS The oligosaccharide extracts from ginseng were permethylated by solid-phase methylation method and then were analyzed by ultra-high-performance liquid chromatography-Q-Orbitrap/MS.
33192120	5	84	theme	standard	713:720	arg1	references					722:731	Several standard references	705:731	Several standard references	705:731	Several standard references were used to further confirm the identification.
33192120	5	85	used	used	738:741	arg2	references					722:731	Several standard references	705:731	Several standard references	705:731	Several standard references were used to further confirm the identification.
33192120	3	86	theme	methylation	454:464	arg1	method					466:471	solid-phase methylation method	442:471	solid-phase methylation method	442:471	METHODS The oligosaccharide extracts from ginseng were permethylated by solid-phase methylation method and then were analyzed by ultra-high-performance liquid chromatography-Q-Orbitrap/MS.
33192120	4	87	theme	mass	687:690	arg1	information					692:702	tandem mass information	680:702	tandem mass information	680:702	The sequence, linkage, and configuration information of oligosaccharides were determined by using accurate m/z value and tandem mass information.
33192120	8	88	theme	ginseng	1274:1280	arg1	samples					1282:1288	ginseng samples	1274:1288	ginseng samples	1274:1288	In the comparison of the oligosaccharide extracts from white ginseng and red ginseng, a clear separation was observed in the partial least squares-discriminate analysis score plot, indicating the sugar differences in these two kinds of ginseng samples.
33192120	12	89	theme	ginseng	2035:2041	arg1	composition					2014:2024	oligosaccharide composition	1998:2024	oligosaccharide composition of white ginseng and red ginseng	1998:2057	The comparison of oligosaccharide composition of white ginseng and red ginseng could help understand the differences in pharmacological activities between these two kinds of ginseng samples from the perspective of glycans.
33192120	4	90	theme	m/z	666:668	arg1	value					670:674	accurate m/z value	657:674	accurate m/z value	657:674	The sequence, linkage, and configuration information of oligosaccharides were determined by using accurate m/z value and tandem mass information.
33192120	7	91	theme	nonreducing	922:932	arg1	erlose					950:955	The nonreducing oligosaccharide erlose	918:955	The nonreducing oligosaccharide erlose among 12 oligosaccharides identified was reported for the first time in ginseng	918:1035	RESULTS The nonreducing oligosaccharide erlose among 12 oligosaccharides identified was reported for the first time in ginseng.
33192120	12	92	theme	red	2047:2049	arg1	ginseng					2051:2057	red ginseng	2047:2057	red ginseng	2047:2057	The comparison of oligosaccharide composition of white ginseng and red ginseng could help understand the differences in pharmacological activities between these two kinds of ginseng samples from the perspective of glycans.
33192120	12	93	theme	white	2029:2033	arg1	ginseng					2035:2041	white ginseng	2029:2041	white ginseng	2029:2041	The comparison of oligosaccharide composition of white ginseng and red ginseng could help understand the differences in pharmacological activities between these two kinds of ginseng samples from the perspective of glycans.
33192120	10	94	theme	maltose	1533:1539	arg1	maltotetraose					1555:1567	maltotetraose	1555:1567	maltotetraose	1555:1567	The contents of oligosaccharides in red ginseng were lower than those in white ginseng, and the contents of maltose, maltotriose, maltotetraose, maltopentaose, maltohexaose, maltoheptaose, maltooctaose, maltononaose, sucrose, and erlose decreased significantly (p < 0.05) in red ginseng.
33192120	10	94	theme	maltose	1533:1539	arg1	maltoheptaose					1599:1611	maltoheptaose	1599:1611	maltoheptaose	1599:1611	The contents of oligosaccharides in red ginseng were lower than those in white ginseng, and the contents of maltose, maltotriose, maltotetraose, maltopentaose, maltohexaose, maltoheptaose, maltooctaose, maltononaose, sucrose, and erlose decreased significantly (p < 0.05) in red ginseng.
33192120	10	94	theme	maltose	1533:1539	arg1	contents					1521:1528	the contents	1517:1528	the contents of maltose	1517:1539	The contents of oligosaccharides in red ginseng were lower than those in white ginseng, and the contents of maltose, maltotriose, maltotetraose, maltopentaose, maltohexaose, maltoheptaose, maltooctaose, maltononaose, sucrose, and erlose decreased significantly (p < 0.05) in red ginseng.
33192120	10	94	theme	maltose	1533:1539	arg1	maltooctaose					1614:1625	maltooctaose	1614:1625	maltooctaose	1614:1625	The contents of oligosaccharides in red ginseng were lower than those in white ginseng, and the contents of maltose, maltotriose, maltotetraose, maltopentaose, maltohexaose, maltoheptaose, maltooctaose, maltononaose, sucrose, and erlose decreased significantly (p < 0.05) in red ginseng.
33192120	10	94	theme	maltose	1533:1539	arg1	maltopentaose					1570:1582	maltopentaose	1570:1582	maltopentaose	1570:1582	The contents of oligosaccharides in red ginseng were lower than those in white ginseng, and the contents of maltose, maltotriose, maltotetraose, maltopentaose, maltohexaose, maltoheptaose, maltooctaose, maltononaose, sucrose, and erlose decreased significantly (p < 0.05) in red ginseng.
33192120	10	94	theme	maltose	1533:1539	arg1	ginseng					1504:1510	white ginseng	1498:1510	white ginseng	1498:1510	The contents of oligosaccharides in red ginseng were lower than those in white ginseng, and the contents of maltose, maltotriose, maltotetraose, maltopentaose, maltohexaose, maltoheptaose, maltooctaose, maltononaose, sucrose, and erlose decreased significantly (p < 0.05) in red ginseng.
33192120	10	94	theme	maltose	1533:1539	arg1	maltohexaose					1585:1596	maltohexaose	1585:1596	maltohexaose	1585:1596	The contents of oligosaccharides in red ginseng were lower than those in white ginseng, and the contents of maltose, maltotriose, maltotetraose, maltopentaose, maltohexaose, maltoheptaose, maltooctaose, maltononaose, sucrose, and erlose decreased significantly (p < 0.05) in red ginseng.
33192120	10	94	theme	maltose	1533:1539	arg1	maltononaose					1628:1639	maltononaose	1628:1639	maltononaose	1628:1639	The contents of oligosaccharides in red ginseng were lower than those in white ginseng, and the contents of maltose, maltotriose, maltotetraose, maltopentaose, maltohexaose, maltoheptaose, maltooctaose, maltononaose, sucrose, and erlose decreased significantly (p < 0.05) in red ginseng.
33192120	10	94	theme	maltose	1533:1539	arg1	maltotriose					1542:1552	maltotriose	1542:1552	maltotriose	1542:1552	The contents of oligosaccharides in red ginseng were lower than those in white ginseng, and the contents of maltose, maltotriose, maltotetraose, maltopentaose, maltohexaose, maltoheptaose, maltooctaose, maltononaose, sucrose, and erlose decreased significantly (p < 0.05) in red ginseng.
33192120	10	94	theme	maltose	1533:1539	arg1	sucrose					1642:1648	sucrose	1642:1648	sucrose	1642:1648	The contents of oligosaccharides in red ginseng were lower than those in white ginseng, and the contents of maltose, maltotriose, maltotetraose, maltopentaose, maltohexaose, maltoheptaose, maltooctaose, maltononaose, sucrose, and erlose decreased significantly (p < 0.05) in red ginseng.
33192120	12	95	theme	oligosaccharide	1998:2012	arg1	composition					2014:2024	oligosaccharide composition	1998:2024	oligosaccharide composition of white ginseng and red ginseng	1998:2057	The comparison of oligosaccharide composition of white ginseng and red ginseng could help understand the differences in pharmacological activities between these two kinds of ginseng samples from the perspective of glycans.
33192120	8	96	located	observed	1147:1154	arg1	plot					1213:1216	the partial least squares-discriminate analysis score plot	1159:1216	the partial least squares-discriminate analysis score plot	1159:1216	In the comparison of the oligosaccharide extracts from white ginseng and red ginseng, a clear separation was observed in the partial least squares-discriminate analysis score plot, indicating the sugar differences in these two kinds of ginseng samples.
33192120	8	96	located	observed	1147:1154	arg2	separation					1132:1141	a clear separation	1124:1141	a clear separation	1124:1141	In the comparison of the oligosaccharide extracts from white ginseng and red ginseng, a clear separation was observed in the partial least squares-discriminate analysis score plot, indicating the sugar differences in these two kinds of ginseng samples.
33192120	11	97	theme	chromatography-tandem	1778:1798	arg1	spectrometry					1805:1816	liquid chromatography-tandem mass spectrometry	1771:1816	liquid chromatography-tandem mass spectrometry	1771:1816	CONCLUSION A solid-phase methylation method combined with liquid chromatography-tandem mass spectrometry was successfully applied to analyze the oligosaccharides in ginseng extracts, which provides the possibility for holistic evaluation of ginseng oligosaccharides.
29649641	11	0	theme	nerve	2183:2187	arg1	regeneration					2189:2200	nerve regeneration	2183:2200	nerve regeneration	2183:2200	However, given their limited availability and lack of bioactive factors, efforts have been made to improve the efficacy of decellularized nerve allograft for nerve regeneration, with limited success.
29649641	12	1	theme	tissue	2251:2256	arg1	matrices					2258:2265	Xenogeneic decellularized tissue matrices	2225:2265	Xenogeneic decellularized tissue matrices	2225:2265	Xenogeneic decellularized tissue matrices or hydrogels have been widely used for surgical applications owing to their ease of harvesting and low immunogenicity.
29649641	2	2	theme	decellularized	351:364	arg1	hydrogels					390:398	decellularized peripheral nerve matrix hydrogels	351:398	decellularized peripheral nerve matrix hydrogels	351:398	However, the preparation of decellularized peripheral nerve matrix hydrogels and their use to repair nerve defects have not been reported.
29649641	11	3	theme	allograft	2169:2177	arg1	efficacy					2136:2143	the efficacy	2132:2143	the efficacy of decellularized nerve allograft for nerve regeneration	2132:2200	However, given their limited availability and lack of bioactive factors, efforts have been made to improve the efficacy of decellularized nerve allograft for nerve regeneration, with limited success.
29649641	7	4	theme	M2	1419:1420	arg1	macrophages					1422:1432	M2 macrophages	1419:1432	M2 macrophages	1419:1432	Additionally, in a 15-mm rat sciatic nerve defect model, pDNM-G was combined with electrospun poly(lactic-acid)-co-poly(trimethylene-carbonate)conduits to bridge the defect, which did not elicit an adverse immune response and promoted the activation of M2 macrophages associated with a constructive remodeling response.
29649641	3	5	theme	porcine	497:503	arg1	pDNM-G					534:539	pDNM-G	534:539	pDNM-G	534:539	Here, we developed a hydrogel from porcine decellularized nerve matrix (pDNM-G), which was confirmed to have minimal DNA content and retain collagen and glycosaminoglycans content, thereby allowing gelatinization.
29649641	3	5	theme	porcine	497:503	arg1	matrix					526:531	porcine decellularized nerve matrix	497:531	porcine decellularized nerve matrix (pDNM-G)	497:540	Here, we developed a hydrogel from porcine decellularized nerve matrix (pDNM-G), which was confirmed to have minimal DNA content and retain collagen and glycosaminoglycans content, thereby allowing gelatinization.
29649641	15	6	theme	residual	2743:2750	arg1	components					2781:2790	residual primary extracellular matrix components	2743:2790	residual primary extracellular matrix components	2743:2790	Our results demonstrate that pDNM-G can support Schwann cell proliferation and peripheral nerve regeneration by means of residual primary extracellular matrix components and nano-fibrous structure features.
29649641	1	7	theme	tissue-specific	268:282	arg1	components					311:320	tissue-specific extracellular matrix (ECM) components	268:320	tissue-specific extracellular matrix (ECM) components	268:320	Decellularized matrix hydrogels derived from tissues or organs have been used for tissue repair due to their biocompatibility, tunability, and tissue-specific extracellular matrix (ECM) components.
29649641	7	8	theme	constructive	1452:1463	arg1	response					1476:1483	a constructive remodeling response	1450:1483	a constructive remodeling response	1450:1483	Additionally, in a 15-mm rat sciatic nerve defect model, pDNM-G was combined with electrospun poly(lactic-acid)-co-poly(trimethylene-carbonate)conduits to bridge the defect, which did not elicit an adverse immune response and promoted the activation of M2 macrophages associated with a constructive remodeling response.
29649641	11	9	theme	limited	2208:2214	arg1	success					2216:2222	limited success	2208:2222	limited success	2208:2222	However, given their limited availability and lack of bioactive factors, efforts have been made to improve the efficacy of decellularized nerve allograft for nerve regeneration, with limited success.
29649641	5	10	contain	contained	972:980	arg2	collagen					1038:1045	collagen I and IV)	1038:1055	collagen	1038:1045	Western blot and liquid chromatography tandem mass spectrometry analysis revealed that the pDNM-G consisted mostly of ECM proteins and contained primary ECM-related proteins, including fibronectin and collagen I and IV).
29649641	5	10	contain	contained	972:980	arg2	fibronectin					1022:1032	fibronectin	1022:1032	fibronectin	1022:1032	Western blot and liquid chromatography tandem mass spectrometry analysis revealed that the pDNM-G consisted mostly of ECM proteins and contained primary ECM-related proteins, including fibronectin and collagen I and IV).
29649641	5	10	contain	contained	972:980	arg2	proteins					1002:1009	primary ECM-related proteins	982:1009	primary ECM-related proteins	982:1009	Western blot and liquid chromatography tandem mass spectrometry analysis revealed that the pDNM-G consisted mostly of ECM proteins and contained primary ECM-related proteins, including fibronectin and collagen I and IV).
29649641	5	10	contain	contained	972:980	arg1	pDNM-G					928:933	the pDNM-G	924:933	the pDNM-G	924:933	Western blot and liquid chromatography tandem mass spectrometry analysis revealed that the pDNM-G consisted mostly of ECM proteins and contained primary ECM-related proteins, including fibronectin and collagen I and IV).
29649641	10	11	theme	peripheral	2000:2009	arg1	injury					2017:2022	peripheral nerve injury	2000:2022	peripheral nerve injury	2000:2022	STATEMENT OF SIGNIFICANCE Decellularized nerve allografts have been widely used to treat peripheral nerve injury.
29649641	5	12	theme	chromatography	861:874	arg1	spectrometry					888:899	liquid chromatography tandem mass spectrometry	854:899	liquid chromatography tandem mass spectrometry	854:899	Western blot and liquid chromatography tandem mass spectrometry analysis revealed that the pDNM-G consisted mostly of ECM proteins and contained primary ECM-related proteins, including fibronectin and collagen I and IV).
29649641	3	13	theme	decellularized	505:518	arg1	pDNM-G					534:539	pDNM-G	534:539	pDNM-G	534:539	Here, we developed a hydrogel from porcine decellularized nerve matrix (pDNM-G), which was confirmed to have minimal DNA content and retain collagen and glycosaminoglycans content, thereby allowing gelatinization.
29649641	3	13	theme	decellularized	505:518	arg1	matrix					526:531	porcine decellularized nerve matrix	497:531	porcine decellularized nerve matrix (pDNM-G)	497:540	Here, we developed a hydrogel from porcine decellularized nerve matrix (pDNM-G), which was confirmed to have minimal DNA content and retain collagen and glycosaminoglycans content, thereby allowing gelatinization.
29649641	15	14	theme	peripheral	2701:2710	arg1	regeneration					2718:2729	peripheral nerve regeneration	2701:2729	peripheral nerve regeneration	2701:2729	Our results demonstrate that pDNM-G can support Schwann cell proliferation and peripheral nerve regeneration by means of residual primary extracellular matrix components and nano-fibrous structure features.
29649641	1	15	theme	tissue	207:212	arg1	repair					214:219	tissue repair	207:219	tissue repair due to their biocompatibility, tunability, and tissue-specific extracellular matrix (ECM) components	207:320	Decellularized matrix hydrogels derived from tissues or organs have been used for tissue repair due to their biocompatibility, tunability, and tissue-specific extracellular matrix (ECM) components.
29649641	7	16	theme	adverse	1364:1370	arg1	response					1379:1386	an adverse immune response	1361:1386	an adverse immune response	1361:1386	Additionally, in a 15-mm rat sciatic nerve defect model, pDNM-G was combined with electrospun poly(lactic-acid)-co-poly(trimethylene-carbonate)conduits to bridge the defect, which did not elicit an adverse immune response and promoted the activation of M2 macrophages associated with a constructive remodeling response.
29649641	5	17	theme	Western	837:843	arg1	blot					845:848	Western blot	837:848	Western blot	837:848	Western blot and liquid chromatography tandem mass spectrometry analysis revealed that the pDNM-G consisted mostly of ECM proteins and contained primary ECM-related proteins, including fibronectin and collagen I and IV).
29649641	11	18	theme	decellularized	2148:2161	arg1	allograft					2169:2177	decellularized nerve allograft	2148:2177	decellularized nerve allograft	2148:2177	However, given their limited availability and lack of bioactive factors, efforts have been made to improve the efficacy of decellularized nerve allograft for nerve regeneration, with limited success.
29649641	6	19	theme	Schwann	1108:1114	arg1	proliferation					1121:1133	Schwann cell proliferation	1108:1133	Schwann cell proliferation	1108:1133	In vitro experiments showed that pDNM-G supported Schwann cell proliferation and preserved cell morphology.
29649641	4	20	theme	similar	721:727	arg1	structure					711:719	a nanofibrous structure	697:719	a nanofibrous structure similar to that of natural ECM	697:750	The pDNM-G exhibited a nanofibrous structure similar to that of natural ECM, and a ∼280-Pa storage modulus at 10 mg/mL similar to that of native neural tissues.
29649641	3	21	theme	minimal	571:577	arg1	content					583:589	minimal DNA content	571:589	minimal DNA content	571:589	Here, we developed a hydrogel from porcine decellularized nerve matrix (pDNM-G), which was confirmed to have minimal DNA content and retain collagen and glycosaminoglycans content, thereby allowing gelatinization.
29649641	1	22	theme	Decellularized	125:138	arg1	hydrogels					147:155	Decellularized matrix hydrogels	125:155	Decellularized matrix hydrogels derived from tissues or organs	125:186	Decellularized matrix hydrogels derived from tissues or organs have been used for tissue repair due to their biocompatibility, tunability, and tissue-specific extracellular matrix (ECM) components.
29649641	1	23	used	used	198:201	arg2	hydrogels					147:155	Decellularized matrix hydrogels	125:155	Decellularized matrix hydrogels derived from tissues or organs	125:186	Decellularized matrix hydrogels derived from tissues or organs have been used for tissue repair due to their biocompatibility, tunability, and tissue-specific extracellular matrix (ECM) components.
29649641	11	24	theme	limited	2046:2052	arg1	availability					2054:2065	their limited availability	2040:2065	their limited availability	2040:2065	However, given their limited availability and lack of bioactive factors, efforts have been made to improve the efficacy of decellularized nerve allograft for nerve regeneration, with limited success.
29649641	5	25	theme	mass	883:886	arg1	spectrometry					888:899	liquid chromatography tandem mass spectrometry	854:899	liquid chromatography tandem mass spectrometry	854:899	Western blot and liquid chromatography tandem mass spectrometry analysis revealed that the pDNM-G consisted mostly of ECM proteins and contained primary ECM-related proteins, including fibronectin and collagen I and IV).
29649641	9	26	theme	ECM	1798:1800	arg1	composition					1802:1812	its preserved ECM composition	1784:1812	its preserved ECM composition	1784:1812	These findings indicated that pDNM-G, with its preserved ECM composition and nanofibrous structure, represents a promising biomaterial for peripheral nerve regeneration.
29649641	4	27	theme	nanofibrous	699:709	arg1	structure					711:719	a nanofibrous structure	697:719	a nanofibrous structure similar to that of natural ECM	697:750	The pDNM-G exhibited a nanofibrous structure similar to that of natural ECM, and a ∼280-Pa storage modulus at 10 mg/mL similar to that of native neural tissues.
29649641	15	28	theme	structure	2809:2817	arg1	features					2819:2826	nano-fibrous structure features	2796:2826	nano-fibrous structure features	2796:2826	Our results demonstrate that pDNM-G can support Schwann cell proliferation and peripheral nerve regeneration by means of residual primary extracellular matrix components and nano-fibrous structure features.
29649641	8	29	theme	electrophysiological	1513:1532	arg1	examinations					1549:1560	electrophysiological and functional examinations	1513:1560	electrophysiological and functional examinations	1513:1560	Morphological analyses and electrophysiological and functional examinations revealed that the regenerative outcomes achieved by pDNM-G were superior to those by empty conduits and closed to those using rat decellularized nerve matrix allograft scaffolds.
29649641	7	30	theme	defect	1209:1214	arg1	model					1216:1220	a 15-mm rat sciatic nerve defect model	1183:1220	a 15-mm rat sciatic nerve defect model	1183:1220	Additionally, in a 15-mm rat sciatic nerve defect model, pDNM-G was combined with electrospun poly(lactic-acid)-co-poly(trimethylene-carbonate)conduits to bridge the defect, which did not elicit an adverse immune response and promoted the activation of M2 macrophages associated with a constructive remodeling response.
29649641	11	31	theme	factors	2089:2095	arg1	lack					2071:2074	lack	2071:2074	lack of bioactive factors	2071:2095	However, given their limited availability and lack of bioactive factors, efforts have been made to improve the efficacy of decellularized nerve allograft for nerve regeneration, with limited success.
29649641	11	31	theme	factors	2089:2095	arg1	availability					2054:2065	their limited availability	2040:2065	their limited availability	2040:2065	However, given their limited availability and lack of bioactive factors, efforts have been made to improve the efficacy of decellularized nerve allograft for nerve regeneration, with limited success.
29649641	8	32	theme	functional	1538:1547	arg1	examinations					1549:1560	electrophysiological and functional examinations	1513:1560	electrophysiological and functional examinations	1513:1560	Morphological analyses and electrophysiological and functional examinations revealed that the regenerative outcomes achieved by pDNM-G were superior to those by empty conduits and closed to those using rat decellularized nerve matrix allograft scaffolds.
29649641	4	33	theme	storage	767:773	arg1	modulus					775:781	a ∼280-Pa storage modulus	757:781	a ∼280-Pa storage modulus at 10 mg/mL similar to that of native neural tissues	757:834	The pDNM-G exhibited a nanofibrous structure similar to that of natural ECM, and a ∼280-Pa storage modulus at 10 mg/mL similar to that of native neural tissues.
29649641	2	34	theme	use	410:412	arg1	preparation					336:346	the preparation	332:346	the preparation of decellularized peripheral nerve matrix hydrogels and their use to repair nerve defects	332:436	However, the preparation of decellularized peripheral nerve matrix hydrogels and their use to repair nerve defects have not been reported.
29649641	0	35	theme	nerve	110:114	arg1	defects					116:122	peripheral nerve defects	99:122	peripheral nerve defects	99:122	Hydrogel derived from porcine decellularized nerve tissue as a promising biomaterial for repairing peripheral nerve defects.
29649641	5	36	theme	primary	982:988	arg1	collagen					1038:1045	collagen I and IV)	1038:1055	collagen	1038:1045	Western blot and liquid chromatography tandem mass spectrometry analysis revealed that the pDNM-G consisted mostly of ECM proteins and contained primary ECM-related proteins, including fibronectin and collagen I and IV).
29649641	5	36	theme	primary	982:988	arg1	proteins					1002:1009	primary ECM-related proteins	982:1009	primary ECM-related proteins	982:1009	Western blot and liquid chromatography tandem mass spectrometry analysis revealed that the pDNM-G consisted mostly of ECM proteins and contained primary ECM-related proteins, including fibronectin and collagen I and IV).
29649641	5	36	theme	primary	982:988	arg1	fibronectin					1022:1032	fibronectin	1022:1032	fibronectin	1022:1032	Western blot and liquid chromatography tandem mass spectrometry analysis revealed that the pDNM-G consisted mostly of ECM proteins and contained primary ECM-related proteins, including fibronectin and collagen I and IV).
29649641	4	37	theme	natural	740:746	arg1	ECM					748:750	natural ECM	740:750	natural ECM	740:750	The pDNM-G exhibited a nanofibrous structure similar to that of natural ECM, and a ∼280-Pa storage modulus at 10 mg/mL similar to that of native neural tissues.
29649641	10	38	theme	nerve	2011:2015	arg1	injury					2017:2022	peripheral nerve injury	2000:2022	peripheral nerve injury	2000:2022	STATEMENT OF SIGNIFICANCE Decellularized nerve allografts have been widely used to treat peripheral nerve injury.
29649641	7	39	theme	15-mm	1185:1189	arg1	model					1216:1220	a 15-mm rat sciatic nerve defect model	1183:1220	a 15-mm rat sciatic nerve defect model	1183:1220	Additionally, in a 15-mm rat sciatic nerve defect model, pDNM-G was combined with electrospun poly(lactic-acid)-co-poly(trimethylene-carbonate)conduits to bridge the defect, which did not elicit an adverse immune response and promoted the activation of M2 macrophages associated with a constructive remodeling response.
29649641	1	40	theme	extracellular	284:296	arg1	components					311:320	tissue-specific extracellular matrix (ECM) components	268:320	tissue-specific extracellular matrix (ECM) components	268:320	Decellularized matrix hydrogels derived from tissues or organs have been used for tissue repair due to their biocompatibility, tunability, and tissue-specific extracellular matrix (ECM) components.
29649641	6	41	theme	In	1058:1059	arg1	experiments					1067:1077	In vitro experiments	1058:1077	In vitro experiments	1058:1077	In vitro experiments showed that pDNM-G supported Schwann cell proliferation and preserved cell morphology.
29649641	9	42	theme	promising	1854:1862	arg1	biomaterial					1864:1874	a promising biomaterial	1852:1874	a promising biomaterial for peripheral nerve regeneration	1852:1908	These findings indicated that pDNM-G, with its preserved ECM composition and nanofibrous structure, represents a promising biomaterial for peripheral nerve regeneration.
29649641	5	43	theme	ECM	955:957	arg1	proteins					959:966	ECM proteins	955:966	ECM proteins	955:966	Western blot and liquid chromatography tandem mass spectrometry analysis revealed that the pDNM-G consisted mostly of ECM proteins and contained primary ECM-related proteins, including fibronectin and collagen I and IV).
29649641	2	44	theme	repair	417:422	arg1	defects					430:436	repair nerve defects	417:436	repair nerve defects	417:436	However, the preparation of decellularized peripheral nerve matrix hydrogels and their use to repair nerve defects have not been reported.
29649641	0	45	theme	decellularized	30:43	arg1	tissue					51:56	porcine decellularized nerve tissue	22:56	porcine decellularized nerve tissue	22:56	Hydrogel derived from porcine decellularized nerve tissue as a promising biomaterial for repairing peripheral nerve defects.
29649641	7	46	theme	trimethylene-carbonate	1286:1307	arg1	conduits					1309:1316	electrospun poly(lactic-acid)-co-poly(trimethylene-carbonate)conduits	1248:1316	electrospun poly(lactic-acid)-co-poly(trimethylene-carbonate)conduits	1248:1316	Additionally, in a 15-mm rat sciatic nerve defect model, pDNM-G was combined with electrospun poly(lactic-acid)-co-poly(trimethylene-carbonate)conduits to bridge the defect, which did not elicit an adverse immune response and promoted the activation of M2 macrophages associated with a constructive remodeling response.
29649641	9	47	theme	nerve	1891:1895	arg1	regeneration					1897:1908	peripheral nerve regeneration	1880:1908	peripheral nerve regeneration	1880:1908	These findings indicated that pDNM-G, with its preserved ECM composition and nanofibrous structure, represents a promising biomaterial for peripheral nerve regeneration.
29649641	4	48	theme	native	814:819	arg1	tissues					828:834	native neural tissues	814:834	native neural tissues	814:834	The pDNM-G exhibited a nanofibrous structure similar to that of natural ECM, and a ∼280-Pa storage modulus at 10 mg/mL similar to that of native neural tissues.
29649641	14	49	theme	decellularized	2523:2536	arg1	pDNM-G					2552:2557	pDNM-G	2552:2557	pDNM-G	2552:2557	In this study, we prepared a porcine decellularized nerve matrix (pDNM-G) and evaluated its potential for promoting nerve regeneration.
29649641	14	49	theme	decellularized	2523:2536	arg1	matrix					2544:2549	a porcine decellularized nerve matrix	2513:2549	a porcine decellularized nerve matrix (pDNM-G)	2513:2558	In this study, we prepared a porcine decellularized nerve matrix (pDNM-G) and evaluated its potential for promoting nerve regeneration.
29649641	7	50	theme	-co-poly	1277:1284	arg1	conduits					1309:1316	electrospun poly(lactic-acid)-co-poly(trimethylene-carbonate)conduits	1248:1316	electrospun poly(lactic-acid)-co-poly(trimethylene-carbonate)conduits	1248:1316	Additionally, in a 15-mm rat sciatic nerve defect model, pDNM-G was combined with electrospun poly(lactic-acid)-co-poly(trimethylene-carbonate)conduits to bridge the defect, which did not elicit an adverse immune response and promoted the activation of M2 macrophages associated with a constructive remodeling response.
29649641	10	51	theme	allografts	1958:1967	arg1	STATEMENT					1911:1919	STATEMENT	1911:1919	STATEMENT OF SIGNIFICANCE Decellularized nerve allografts	1911:1967	STATEMENT OF SIGNIFICANCE Decellularized nerve allografts have been widely used to treat peripheral nerve injury.
29649641	12	52	theme	Xenogeneic	2225:2234	arg1	matrices					2258:2265	Xenogeneic decellularized tissue matrices	2225:2265	Xenogeneic decellularized tissue matrices	2225:2265	Xenogeneic decellularized tissue matrices or hydrogels have been widely used for surgical applications owing to their ease of harvesting and low immunogenicity.
29649641	7	53	theme	lactic-acid	1265:1275	arg1	conduits					1309:1316	electrospun poly(lactic-acid)-co-poly(trimethylene-carbonate)conduits	1248:1316	electrospun poly(lactic-acid)-co-poly(trimethylene-carbonate)conduits	1248:1316	Additionally, in a 15-mm rat sciatic nerve defect model, pDNM-G was combined with electrospun poly(lactic-acid)-co-poly(trimethylene-carbonate)conduits to bridge the defect, which did not elicit an adverse immune response and promoted the activation of M2 macrophages associated with a constructive remodeling response.
29649641	10	54	theme	Decellularized	1937:1950	arg1	allografts					1958:1967	SIGNIFICANCE Decellularized nerve allografts	1924:1967	SIGNIFICANCE Decellularized nerve allografts	1924:1967	STATEMENT OF SIGNIFICANCE Decellularized nerve allografts have been widely used to treat peripheral nerve injury.
29649641	4	55	theme	similar	795:801	arg1	10 mg/mL					786:793	10 mg/mL	786:793	10 mg/mL similar to that of native neural tissues	786:834	The pDNM-G exhibited a nanofibrous structure similar to that of natural ECM, and a ∼280-Pa storage modulus at 10 mg/mL similar to that of native neural tissues.
29649641	3	56	theme	nerve	520:524	arg1	pDNM-G					534:539	pDNM-G	534:539	pDNM-G	534:539	Here, we developed a hydrogel from porcine decellularized nerve matrix (pDNM-G), which was confirmed to have minimal DNA content and retain collagen and glycosaminoglycans content, thereby allowing gelatinization.
29649641	3	56	theme	nerve	520:524	arg1	matrix					526:531	porcine decellularized nerve matrix	497:531	porcine decellularized nerve matrix (pDNM-G)	497:540	Here, we developed a hydrogel from porcine decellularized nerve matrix (pDNM-G), which was confirmed to have minimal DNA content and retain collagen and glycosaminoglycans content, thereby allowing gelatinization.
29649641	8	57	theme	rat	1688:1690	arg1	scaffolds					1730:1738	rat decellularized nerve matrix allograft scaffolds	1688:1738	rat decellularized nerve matrix allograft scaffolds	1688:1738	Morphological analyses and electrophysiological and functional examinations revealed that the regenerative outcomes achieved by pDNM-G were superior to those by empty conduits and closed to those using rat decellularized nerve matrix allograft scaffolds.
29649641	7	58	theme	poly	1260:1263	arg1	conduits					1309:1316	electrospun poly(lactic-acid)-co-poly(trimethylene-carbonate)conduits	1248:1316	electrospun poly(lactic-acid)-co-poly(trimethylene-carbonate)conduits	1248:1316	Additionally, in a 15-mm rat sciatic nerve defect model, pDNM-G was combined with electrospun poly(lactic-acid)-co-poly(trimethylene-carbonate)conduits to bridge the defect, which did not elicit an adverse immune response and promoted the activation of M2 macrophages associated with a constructive remodeling response.
29649641	2	59	theme	peripheral	366:375	arg1	hydrogels					390:398	decellularized peripheral nerve matrix hydrogels	351:398	decellularized peripheral nerve matrix hydrogels	351:398	However, the preparation of decellularized peripheral nerve matrix hydrogels and their use to repair nerve defects have not been reported.
29649641	8	60	theme	nerve	1707:1711	arg1	scaffolds					1730:1738	rat decellularized nerve matrix allograft scaffolds	1688:1738	rat decellularized nerve matrix allograft scaffolds	1688:1738	Morphological analyses and electrophysiological and functional examinations revealed that the regenerative outcomes achieved by pDNM-G were superior to those by empty conduits and closed to those using rat decellularized nerve matrix allograft scaffolds.
29649641	7	61	theme	remodeling	1465:1474	arg1	response					1476:1483	a constructive remodeling response	1450:1483	a constructive remodeling response	1450:1483	Additionally, in a 15-mm rat sciatic nerve defect model, pDNM-G was combined with electrospun poly(lactic-acid)-co-poly(trimethylene-carbonate)conduits to bridge the defect, which did not elicit an adverse immune response and promoted the activation of M2 macrophages associated with a constructive remodeling response.
29649641	3	62	contain	have	566:569	arg1	hydrogel					483:490	a hydrogel	481:490	a hydrogel	481:490	Here, we developed a hydrogel from porcine decellularized nerve matrix (pDNM-G), which was confirmed to have minimal DNA content and retain collagen and glycosaminoglycans content, thereby allowing gelatinization.
29649641	3	62	contain	have	566:569	arg2	content					583:589	minimal DNA content	571:589	minimal DNA content	571:589	Here, we developed a hydrogel from porcine decellularized nerve matrix (pDNM-G), which was confirmed to have minimal DNA content and retain collagen and glycosaminoglycans content, thereby allowing gelatinization.
29649641	15	63	theme	matrix	2774:2779	arg1	components					2781:2790	residual primary extracellular matrix components	2743:2790	residual primary extracellular matrix components	2743:2790	Our results demonstrate that pDNM-G can support Schwann cell proliferation and peripheral nerve regeneration by means of residual primary extracellular matrix components and nano-fibrous structure features.
29649641	11	64	theme	bioactive	2079:2087	arg1	factors					2089:2095	bioactive factors	2079:2095	bioactive factors	2079:2095	However, given their limited availability and lack of bioactive factors, efforts have been made to improve the efficacy of decellularized nerve allograft for nerve regeneration, with limited success.
29649641	8	65	theme	allograft	1720:1728	arg1	scaffolds					1730:1738	rat decellularized nerve matrix allograft scaffolds	1688:1738	rat decellularized nerve matrix allograft scaffolds	1688:1738	Morphological analyses and electrophysiological and functional examinations revealed that the regenerative outcomes achieved by pDNM-G were superior to those by empty conduits and closed to those using rat decellularized nerve matrix allograft scaffolds.
29649641	10	66	used	used	1986:1989	arg2	STATEMENT					1911:1919	STATEMENT	1911:1919	STATEMENT OF SIGNIFICANCE Decellularized nerve allografts	1911:1967	STATEMENT OF SIGNIFICANCE Decellularized nerve allografts have been widely used to treat peripheral nerve injury.
29649641	7	67	theme	macrophages	1422:1432	arg1	activation					1405:1414	the activation	1401:1414	the activation of M2 macrophages associated with a constructive remodeling response	1401:1483	Additionally, in a 15-mm rat sciatic nerve defect model, pDNM-G was combined with electrospun poly(lactic-acid)-co-poly(trimethylene-carbonate)conduits to bridge the defect, which did not elicit an adverse immune response and promoted the activation of M2 macrophages associated with a constructive remodeling response.
29649641	1	68	attach	derived	157:163	arg1	tissues					170:176	tissues	170:176	tissues	170:176	Decellularized matrix hydrogels derived from tissues or organs have been used for tissue repair due to their biocompatibility, tunability, and tissue-specific extracellular matrix (ECM) components.
29649641	1	68	attach	derived	157:163	arg1	organs					181:186	organs	181:186	organs	181:186	Decellularized matrix hydrogels derived from tissues or organs have been used for tissue repair due to their biocompatibility, tunability, and tissue-specific extracellular matrix (ECM) components.
29649641	1	68	attach	derived	157:163	arg2	hydrogels					147:155	Decellularized matrix hydrogels	125:155	Decellularized matrix hydrogels derived from tissues or organs	125:186	Decellularized matrix hydrogels derived from tissues or organs have been used for tissue repair due to their biocompatibility, tunability, and tissue-specific extracellular matrix (ECM) components.
29649641	3	69	from	matrix	526:531	arg1	hydrogel					483:490	a hydrogel	481:490	a hydrogel	481:490	Here, we developed a hydrogel from porcine decellularized nerve matrix (pDNM-G), which was confirmed to have minimal DNA content and retain collagen and glycosaminoglycans content, thereby allowing gelatinization.
29649641	15	70	theme	primary	2752:2758	arg1	components					2781:2790	residual primary extracellular matrix components	2743:2790	residual primary extracellular matrix components	2743:2790	Our results demonstrate that pDNM-G can support Schwann cell proliferation and peripheral nerve regeneration by means of residual primary extracellular matrix components and nano-fibrous structure features.
29649641	5	71	theme	blot	845:848	arg1	analysis					901:908	Western blot and liquid chromatography tandem mass spectrometry analysis	837:908	Western blot and liquid chromatography tandem mass spectrometry analysis	837:908	Western blot and liquid chromatography tandem mass spectrometry analysis revealed that the pDNM-G consisted mostly of ECM proteins and contained primary ECM-related proteins, including fibronectin and collagen I and IV).
29649641	4	72	theme	neural	821:826	arg1	tissues					828:834	native neural tissues	814:834	native neural tissues	814:834	The pDNM-G exhibited a nanofibrous structure similar to that of natural ECM, and a ∼280-Pa storage modulus at 10 mg/mL similar to that of native neural tissues.
29649641	13	73	theme	matrix	2418:2423	arg1	hydrogels					2425:2433	decellularized tissue matrix hydrogels	2396:2433	decellularized tissue matrix hydrogels	2396:2433	Moreover, decellularized tissue matrix hydrogels show good biocompatibility and are highly tunable.
29649641	5	74	theme	liquid	854:859	arg1	spectrometry					888:899	liquid chromatography tandem mass spectrometry	854:899	liquid chromatography tandem mass spectrometry	854:899	Western blot and liquid chromatography tandem mass spectrometry analysis revealed that the pDNM-G consisted mostly of ECM proteins and contained primary ECM-related proteins, including fibronectin and collagen I and IV).
29649641	2	75	theme	matrix	383:388	arg1	hydrogels					390:398	decellularized peripheral nerve matrix hydrogels	351:398	decellularized peripheral nerve matrix hydrogels	351:398	However, the preparation of decellularized peripheral nerve matrix hydrogels and their use to repair nerve defects have not been reported.
29649641	6	76	theme	cell	1149:1152	arg1	morphology					1154:1163	cell morphology	1149:1163	cell morphology	1149:1163	In vitro experiments showed that pDNM-G supported Schwann cell proliferation and preserved cell morphology.
29649641	3	77	theme	DNA	579:581	arg1	content					583:589	minimal DNA content	571:589	minimal DNA content	571:589	Here, we developed a hydrogel from porcine decellularized nerve matrix (pDNM-G), which was confirmed to have minimal DNA content and retain collagen and glycosaminoglycans content, thereby allowing gelatinization.
29649641	13	78	theme	good	2440:2443	arg1	biocompatibility					2445:2460	good biocompatibility	2440:2460	good biocompatibility	2440:2460	Moreover, decellularized tissue matrix hydrogels show good biocompatibility and are highly tunable.
29649641	13	79	theme	decellularized	2396:2409	arg1	hydrogels					2425:2433	decellularized tissue matrix hydrogels	2396:2433	decellularized tissue matrix hydrogels	2396:2433	Moreover, decellularized tissue matrix hydrogels show good biocompatibility and are highly tunable.
29649641	7	80	theme	immune	1372:1377	arg1	response					1379:1386	an adverse immune response	1361:1386	an adverse immune response	1361:1386	Additionally, in a 15-mm rat sciatic nerve defect model, pDNM-G was combined with electrospun poly(lactic-acid)-co-poly(trimethylene-carbonate)conduits to bridge the defect, which did not elicit an adverse immune response and promoted the activation of M2 macrophages associated with a constructive remodeling response.
29649641	7	81	theme	nerve	1203:1207	arg1	model					1216:1220	a 15-mm rat sciatic nerve defect model	1183:1220	a 15-mm rat sciatic nerve defect model	1183:1220	Additionally, in a 15-mm rat sciatic nerve defect model, pDNM-G was combined with electrospun poly(lactic-acid)-co-poly(trimethylene-carbonate)conduits to bridge the defect, which did not elicit an adverse immune response and promoted the activation of M2 macrophages associated with a constructive remodeling response.
29649641	12	82	theme	immunogenicity	2370:2383	arg1	ease					2343:2346	their ease	2337:2346	their ease of harvesting and low immunogenicity	2337:2383	Xenogeneic decellularized tissue matrices or hydrogels have been widely used for surgical applications owing to their ease of harvesting and low immunogenicity.
29649641	11	83	theme	nerve	2163:2167	arg1	allograft					2169:2177	decellularized nerve allograft	2148:2177	decellularized nerve allograft	2148:2177	However, given their limited availability and lack of bioactive factors, efforts have been made to improve the efficacy of decellularized nerve allograft for nerve regeneration, with limited success.
29649641	3	84	theme	collagen	602:609	arg1	content					634:640	collagen and glycosaminoglycans content	602:640	collagen and glycosaminoglycans content	602:640	Here, we developed a hydrogel from porcine decellularized nerve matrix (pDNM-G), which was confirmed to have minimal DNA content and retain collagen and glycosaminoglycans content, thereby allowing gelatinization.
29649641	1	85	theme	matrix	140:145	arg1	hydrogels					147:155	Decellularized matrix hydrogels	125:155	Decellularized matrix hydrogels derived from tissues or organs	125:186	Decellularized matrix hydrogels derived from tissues or organs have been used for tissue repair due to their biocompatibility, tunability, and tissue-specific extracellular matrix (ECM) components.
29649641	8	86	theme	Morphological	1486:1498	arg1	analyses					1500:1507	Morphological analyses	1486:1507	Morphological analyses	1486:1507	Morphological analyses and electrophysiological and functional examinations revealed that the regenerative outcomes achieved by pDNM-G were superior to those by empty conduits and closed to those using rat decellularized nerve matrix allograft scaffolds.
29649641	0	87	theme	promising	63:71	arg1	biomaterial					73:83	a promising biomaterial	61:83	a promising biomaterial for repairing peripheral nerve defects	61:122	Hydrogel derived from porcine decellularized nerve tissue as a promising biomaterial for repairing peripheral nerve defects.
29649641	4	88	from	10 mg/mL	786:793	arg1	modulus					775:781	a ∼280-Pa storage modulus	757:781	a ∼280-Pa storage modulus at 10 mg/mL similar to that of native neural tissues	757:834	The pDNM-G exhibited a nanofibrous structure similar to that of natural ECM, and a ∼280-Pa storage modulus at 10 mg/mL similar to that of native neural tissues.
29649641	4	88	from	10 mg/mL	786:793	arg1	structure					711:719	a nanofibrous structure	697:719	a nanofibrous structure similar to that of natural ECM	697:750	The pDNM-G exhibited a nanofibrous structure similar to that of natural ECM, and a ∼280-Pa storage modulus at 10 mg/mL similar to that of native neural tissues.
29649641	5	89	theme	tandem	876:881	arg1	spectrometry					888:899	liquid chromatography tandem mass spectrometry	854:899	liquid chromatography tandem mass spectrometry	854:899	Western blot and liquid chromatography tandem mass spectrometry analysis revealed that the pDNM-G consisted mostly of ECM proteins and contained primary ECM-related proteins, including fibronectin and collagen I and IV).
29649641	9	90	theme	preserved	1788:1796	arg1	composition					1802:1812	its preserved ECM composition	1784:1812	its preserved ECM composition	1784:1812	These findings indicated that pDNM-G, with its preserved ECM composition and nanofibrous structure, represents a promising biomaterial for peripheral nerve regeneration.
29649641	12	91	theme	low	2366:2368	arg1	immunogenicity					2370:2383	low immunogenicity	2366:2383	low immunogenicity	2366:2383	Xenogeneic decellularized tissue matrices or hydrogels have been widely used for surgical applications owing to their ease of harvesting and low immunogenicity.
29649641	15	92	theme	nano-fibrous	2796:2807	arg1	features					2819:2826	nano-fibrous structure features	2796:2826	nano-fibrous structure features	2796:2826	Our results demonstrate that pDNM-G can support Schwann cell proliferation and peripheral nerve regeneration by means of residual primary extracellular matrix components and nano-fibrous structure features.
29649641	5	93	theme	spectrometry	888:899	arg1	analysis					901:908	Western blot and liquid chromatography tandem mass spectrometry analysis	837:908	Western blot and liquid chromatography tandem mass spectrometry analysis	837:908	Western blot and liquid chromatography tandem mass spectrometry analysis revealed that the pDNM-G consisted mostly of ECM proteins and contained primary ECM-related proteins, including fibronectin and collagen I and IV).
29649641	0	94	theme	peripheral	99:108	arg1	defects					116:122	peripheral nerve defects	99:122	peripheral nerve defects	99:122	Hydrogel derived from porcine decellularized nerve tissue as a promising biomaterial for repairing peripheral nerve defects.
29649641	9	95	theme	nanofibrous	1818:1828	arg1	structure					1830:1838	nanofibrous structure	1818:1838	nanofibrous structure	1818:1838	These findings indicated that pDNM-G, with its preserved ECM composition and nanofibrous structure, represents a promising biomaterial for peripheral nerve regeneration.
29649641	12	96	theme	surgical	2306:2313	arg1	applications					2315:2326	surgical applications	2306:2326	surgical applications owing to their ease of harvesting and low immunogenicity	2306:2383	Xenogeneic decellularized tissue matrices or hydrogels have been widely used for surgical applications owing to their ease of harvesting and low immunogenicity.
29649641	6	97	theme	cell	1116:1119	arg1	proliferation					1121:1133	Schwann cell proliferation	1108:1133	Schwann cell proliferation	1108:1133	In vitro experiments showed that pDNM-G supported Schwann cell proliferation and preserved cell morphology.
29649641	7	98	theme	rat	1191:1193	arg1	model					1216:1220	a 15-mm rat sciatic nerve defect model	1183:1220	a 15-mm rat sciatic nerve defect model	1183:1220	Additionally, in a 15-mm rat sciatic nerve defect model, pDNM-G was combined with electrospun poly(lactic-acid)-co-poly(trimethylene-carbonate)conduits to bridge the defect, which did not elicit an adverse immune response and promoted the activation of M2 macrophages associated with a constructive remodeling response.
29649641	15	99	theme	Schwann	2670:2676	arg1	proliferation					2683:2695	Schwann cell proliferation	2670:2695	Schwann cell proliferation	2670:2695	Our results demonstrate that pDNM-G can support Schwann cell proliferation and peripheral nerve regeneration by means of residual primary extracellular matrix components and nano-fibrous structure features.
29649641	12	100	theme	harvesting	2351:2360	arg1	ease					2343:2346	their ease	2337:2346	their ease of harvesting and low immunogenicity	2337:2383	Xenogeneic decellularized tissue matrices or hydrogels have been widely used for surgical applications owing to their ease of harvesting and low immunogenicity.
29649641	4	101	theme	∼280-Pa	759:765	arg1	modulus					775:781	a ∼280-Pa storage modulus	757:781	a ∼280-Pa storage modulus at 10 mg/mL similar to that of native neural tissues	757:834	The pDNM-G exhibited a nanofibrous structure similar to that of natural ECM, and a ∼280-Pa storage modulus at 10 mg/mL similar to that of native neural tissues.
29649641	15	102	theme	extracellular	2760:2772	arg1	components					2781:2790	residual primary extracellular matrix components	2743:2790	residual primary extracellular matrix components	2743:2790	Our results demonstrate that pDNM-G can support Schwann cell proliferation and peripheral nerve regeneration by means of residual primary extracellular matrix components and nano-fibrous structure features.
29649641	8	103	theme	regenerative	1580:1591	arg1	outcomes					1593:1600	the regenerative outcomes	1576:1600	the regenerative outcomes achieved by pDNM-G	1576:1619	Morphological analyses and electrophysiological and functional examinations revealed that the regenerative outcomes achieved by pDNM-G were superior to those by empty conduits and closed to those using rat decellularized nerve matrix allograft scaffolds.
29649641	8	103	theme	regenerative	1580:1591	arg1	superior					1626:1633	superior	1626:1633	superior	1626:1633	Morphological analyses and electrophysiological and functional examinations revealed that the regenerative outcomes achieved by pDNM-G were superior to those by empty conduits and closed to those using rat decellularized nerve matrix allograft scaffolds.
29649641	6	104	dep	In	1058:1059	arg1	vitro					1061:1065	vitro	1061:1065	vitro	1061:1065	In vitro experiments showed that pDNM-G supported Schwann cell proliferation and preserved cell morphology.
29649641	2	105	theme	hydrogels	390:398	arg1	preparation					336:346	the preparation	332:346	the preparation of decellularized peripheral nerve matrix hydrogels and their use to repair nerve defects	332:436	However, the preparation of decellularized peripheral nerve matrix hydrogels and their use to repair nerve defects have not been reported.
29649641	0	106	theme	porcine	22:28	arg1	tissue					51:56	porcine decellularized nerve tissue	22:56	porcine decellularized nerve tissue	22:56	Hydrogel derived from porcine decellularized nerve tissue as a promising biomaterial for repairing peripheral nerve defects.
29649641	1	107	theme	matrix	298:303	arg1	components					311:320	tissue-specific extracellular matrix (ECM) components	268:320	tissue-specific extracellular matrix (ECM) components	268:320	Decellularized matrix hydrogels derived from tissues or organs have been used for tissue repair due to their biocompatibility, tunability, and tissue-specific extracellular matrix (ECM) components.
29649641	12	108	used	used	2297:2300	arg2	hydrogels					2270:2278	hydrogels	2270:2278	hydrogels	2270:2278	Xenogeneic decellularized tissue matrices or hydrogels have been widely used for surgical applications owing to their ease of harvesting and low immunogenicity.
29649641	12	108	used	used	2297:2300	arg2	matrices					2258:2265	Xenogeneic decellularized tissue matrices	2225:2265	Xenogeneic decellularized tissue matrices	2225:2265	Xenogeneic decellularized tissue matrices or hydrogels have been widely used for surgical applications owing to their ease of harvesting and low immunogenicity.
29649641	10	109	theme	SIGNIFICANCE	1924:1935	arg1	allografts					1958:1967	SIGNIFICANCE Decellularized nerve allografts	1924:1967	SIGNIFICANCE Decellularized nerve allografts	1924:1967	STATEMENT OF SIGNIFICANCE Decellularized nerve allografts have been widely used to treat peripheral nerve injury.
29649641	15	110	theme	nerve	2712:2716	arg1	regeneration					2718:2729	peripheral nerve regeneration	2701:2729	peripheral nerve regeneration	2701:2729	Our results demonstrate that pDNM-G can support Schwann cell proliferation and peripheral nerve regeneration by means of residual primary extracellular matrix components and nano-fibrous structure features.
29649641	2	111	theme	nerve	424:428	arg1	defects					430:436	repair nerve defects	417:436	repair nerve defects	417:436	However, the preparation of decellularized peripheral nerve matrix hydrogels and their use to repair nerve defects have not been reported.
29649641	0	112	theme	nerve	45:49	arg1	tissue					51:56	porcine decellularized nerve tissue	22:56	porcine decellularized nerve tissue	22:56	Hydrogel derived from porcine decellularized nerve tissue as a promising biomaterial for repairing peripheral nerve defects.
29649641	1	113	theme	ECM	306:308	arg1	components					311:320	tissue-specific extracellular matrix (ECM) components	268:320	tissue-specific extracellular matrix (ECM) components	268:320	Decellularized matrix hydrogels derived from tissues or organs have been used for tissue repair due to their biocompatibility, tunability, and tissue-specific extracellular matrix (ECM) components.
29649641	15	114	theme	cell	2678:2681	arg1	proliferation					2683:2695	Schwann cell proliferation	2670:2695	Schwann cell proliferation	2670:2695	Our results demonstrate that pDNM-G can support Schwann cell proliferation and peripheral nerve regeneration by means of residual primary extracellular matrix components and nano-fibrous structure features.
29649641	9	115	theme	peripheral	1880:1889	arg1	regeneration					1897:1908	peripheral nerve regeneration	1880:1908	peripheral nerve regeneration	1880:1908	These findings indicated that pDNM-G, with its preserved ECM composition and nanofibrous structure, represents a promising biomaterial for peripheral nerve regeneration.
29649641	3	116	theme	glycosaminoglycans	615:632	arg1	content					634:640	collagen and glycosaminoglycans content	602:640	collagen and glycosaminoglycans content	602:640	Here, we developed a hydrogel from porcine decellularized nerve matrix (pDNM-G), which was confirmed to have minimal DNA content and retain collagen and glycosaminoglycans content, thereby allowing gelatinization.
29649641	14	117	theme	nerve	2538:2542	arg1	pDNM-G					2552:2557	pDNM-G	2552:2557	pDNM-G	2552:2557	In this study, we prepared a porcine decellularized nerve matrix (pDNM-G) and evaluated its potential for promoting nerve regeneration.
29649641	14	117	theme	nerve	2538:2542	arg1	matrix					2544:2549	a porcine decellularized nerve matrix	2513:2549	a porcine decellularized nerve matrix (pDNM-G)	2513:2558	In this study, we prepared a porcine decellularized nerve matrix (pDNM-G) and evaluated its potential for promoting nerve regeneration.
29649641	7	118	theme	sciatic	1195:1201	arg1	model					1216:1220	a 15-mm rat sciatic nerve defect model	1183:1220	a 15-mm rat sciatic nerve defect model	1183:1220	Additionally, in a 15-mm rat sciatic nerve defect model, pDNM-G was combined with electrospun poly(lactic-acid)-co-poly(trimethylene-carbonate)conduits to bridge the defect, which did not elicit an adverse immune response and promoted the activation of M2 macrophages associated with a constructive remodeling response.
29649641	14	119	theme	porcine	2515:2521	arg1	pDNM-G					2552:2557	pDNM-G	2552:2557	pDNM-G	2552:2557	In this study, we prepared a porcine decellularized nerve matrix (pDNM-G) and evaluated its potential for promoting nerve regeneration.
29649641	14	119	theme	porcine	2515:2521	arg1	matrix					2544:2549	a porcine decellularized nerve matrix	2513:2549	a porcine decellularized nerve matrix (pDNM-G)	2513:2558	In this study, we prepared a porcine decellularized nerve matrix (pDNM-G) and evaluated its potential for promoting nerve regeneration.
29649641	14	120	theme	nerve	2602:2606	arg1	regeneration					2608:2619	nerve regeneration	2602:2619	nerve regeneration	2602:2619	In this study, we prepared a porcine decellularized nerve matrix (pDNM-G) and evaluated its potential for promoting nerve regeneration.
29649641	5	121	theme	ECM-related	990:1000	arg1	collagen					1038:1045	collagen I and IV)	1038:1055	collagen	1038:1045	Western blot and liquid chromatography tandem mass spectrometry analysis revealed that the pDNM-G consisted mostly of ECM proteins and contained primary ECM-related proteins, including fibronectin and collagen I and IV).
29649641	5	121	theme	ECM-related	990:1000	arg1	proteins					1002:1009	primary ECM-related proteins	982:1009	primary ECM-related proteins	982:1009	Western blot and liquid chromatography tandem mass spectrometry analysis revealed that the pDNM-G consisted mostly of ECM proteins and contained primary ECM-related proteins, including fibronectin and collagen I and IV).
29649641	5	121	theme	ECM-related	990:1000	arg1	fibronectin					1022:1032	fibronectin	1022:1032	fibronectin	1022:1032	Western blot and liquid chromatography tandem mass spectrometry analysis revealed that the pDNM-G consisted mostly of ECM proteins and contained primary ECM-related proteins, including fibronectin and collagen I and IV).
29649641	10	122	theme	nerve	1952:1956	arg1	allografts					1958:1967	SIGNIFICANCE Decellularized nerve allografts	1924:1967	SIGNIFICANCE Decellularized nerve allografts	1924:1967	STATEMENT OF SIGNIFICANCE Decellularized nerve allografts have been widely used to treat peripheral nerve injury.
29649641	8	123	theme	decellularized	1692:1705	arg1	scaffolds					1730:1738	rat decellularized nerve matrix allograft scaffolds	1688:1738	rat decellularized nerve matrix allograft scaffolds	1688:1738	Morphological analyses and electrophysiological and functional examinations revealed that the regenerative outcomes achieved by pDNM-G were superior to those by empty conduits and closed to those using rat decellularized nerve matrix allograft scaffolds.
29649641	13	124	theme	tissue	2411:2416	arg1	hydrogels					2425:2433	decellularized tissue matrix hydrogels	2396:2433	decellularized tissue matrix hydrogels	2396:2433	Moreover, decellularized tissue matrix hydrogels show good biocompatibility and are highly tunable.
29649641	7	125	theme	electrospun	1248:1258	arg1	conduits					1309:1316	electrospun poly(lactic-acid)-co-poly(trimethylene-carbonate)conduits	1248:1316	electrospun poly(lactic-acid)-co-poly(trimethylene-carbonate)conduits	1248:1316	Additionally, in a 15-mm rat sciatic nerve defect model, pDNM-G was combined with electrospun poly(lactic-acid)-co-poly(trimethylene-carbonate)conduits to bridge the defect, which did not elicit an adverse immune response and promoted the activation of M2 macrophages associated with a constructive remodeling response.
29649641	8	126	theme	empty	1647:1651	arg1	conduits					1653:1660	empty conduits	1647:1660	empty conduits	1647:1660	Morphological analyses and electrophysiological and functional examinations revealed that the regenerative outcomes achieved by pDNM-G were superior to those by empty conduits and closed to those using rat decellularized nerve matrix allograft scaffolds.
29649641	12	127	theme	decellularized	2236:2249	arg1	matrices					2258:2265	Xenogeneic decellularized tissue matrices	2225:2265	Xenogeneic decellularized tissue matrices	2225:2265	Xenogeneic decellularized tissue matrices or hydrogels have been widely used for surgical applications owing to their ease of harvesting and low immunogenicity.
29649641	2	128	theme	nerve	377:381	arg1	hydrogels					390:398	decellularized peripheral nerve matrix hydrogels	351:398	decellularized peripheral nerve matrix hydrogels	351:398	However, the preparation of decellularized peripheral nerve matrix hydrogels and their use to repair nerve defects have not been reported.
29649641	8	129	theme	matrix	1713:1718	arg1	scaffolds					1730:1738	rat decellularized nerve matrix allograft scaffolds	1688:1738	rat decellularized nerve matrix allograft scaffolds	1688:1738	Morphological analyses and electrophysiological and functional examinations revealed that the regenerative outcomes achieved by pDNM-G were superior to those by empty conduits and closed to those using rat decellularized nerve matrix allograft scaffolds.
30979031	2	0	from	colitis	495:501	arg1	mice					506:509	mice	506:509	mice	506:509	To understand whether probiotics could simultaneously alleviate anxiety/depression and colitis, we examined their effects on immobilization stress (IS)-induced anxiety/depression and colitis in mice.
30979031	10	1	theme	LPS-induced	1527:1537	arg1	cells					1547:1551	LPS-induced SH-SY5Y cells	1527:1551	LPS-induced SH-SY5Y cells	1527:1551	They also induced BDNF expression in LPS-induced SH-SY5Y cells in vitro.
30979031	8	2	theme	colon	1265:1269	arg1	shortening					1271:1280	IS-induced colon shortening	1254:1280	IS-induced colon shortening	1254:1280	NK33 and/or NK98 treatments suppressed IS-induced colon shortening, myeloperoxidase activity, infiltration of CD11b⁺/CD11c⁺ cells, and IL-6 expression in the colon.
30979031	6	3	theme	LPS⁺/CD11b⁺	1005:1015	arg1	cells					1017:1021	LPS⁺/CD11b⁺ cells	1005:1021	LPS⁺/CD11b⁺ cells (activated microglia)	1005:1043	Treatment with NK33 and/or NK98, which were orally gavaged in mice before or after IS treatment, significantly suppressed the occurrence and development of anxiety/depression, infiltration of Iba1⁺ and LPS⁺/CD11b⁺ cells (activated microglia) into the hippocampus, and corticosterone, IL-6, and LPS levels in the blood.
30979031	6	3	theme	LPS⁺/CD11b⁺	1005:1015	arg1	microglia					1034:1042	activated microglia	1024:1042	activated microglia	1024:1042	Treatment with NK33 and/or NK98, which were orally gavaged in mice before or after IS treatment, significantly suppressed the occurrence and development of anxiety/depression, infiltration of Iba1⁺ and LPS⁺/CD11b⁺ cells (activated microglia) into the hippocampus, and corticosterone, IL-6, and LPS levels in the blood.
30979031	6	4	from	infiltration	979:990	arg1	blood					1115:1119	the blood	1111:1119	the blood	1111:1119	Treatment with NK33 and/or NK98, which were orally gavaged in mice before or after IS treatment, significantly suppressed the occurrence and development of anxiety/depression, infiltration of Iba1⁺ and LPS⁺/CD11b⁺ cells (activated microglia) into the hippocampus, and corticosterone, IL-6, and LPS levels in the blood.
30979031	11	5	theme	colitis	1676:1682	arg1	occurrence					1623:1632	occurrence	1623:1632	occurrence	1623:1632	In conclusion, NK33 and NK98 synergistically alleviated the occurrence and development of anxiety/depression and colitis through the regulation of gut immune responses and microbiota composition.
30979031	11	5	theme	colitis	1676:1682	arg1	development					1638:1648	development	1638:1648	development	1638:1648	In conclusion, NK33 and NK98 synergistically alleviated the occurrence and development of anxiety/depression and colitis through the regulation of gut immune responses and microbiota composition.
30979031	6	6	theme	Iba1⁺	995:999	arg1	levels					1101:1106	LPS levels	1097:1106	LPS levels in the blood	1097:1119	Treatment with NK33 and/or NK98, which were orally gavaged in mice before or after IS treatment, significantly suppressed the occurrence and development of anxiety/depression, infiltration of Iba1⁺ and LPS⁺/CD11b⁺ cells (activated microglia) into the hippocampus, and corticosterone, IL-6, and LPS levels in the blood.
30979031	6	6	theme	Iba1⁺	995:999	arg1	infiltration					979:990	infiltration	979:990	infiltration of Iba1⁺ and LPS⁺/CD11b⁺ cells (activated microglia) into the hippocampus	979:1064	Treatment with NK33 and/or NK98, which were orally gavaged in mice before or after IS treatment, significantly suppressed the occurrence and development of anxiety/depression, infiltration of Iba1⁺ and LPS⁺/CD11b⁺ cells (activated microglia) into the hippocampus, and corticosterone, IL-6, and LPS levels in the blood.
30979031	6	6	theme	Iba1⁺	995:999	arg1	anxiety/depression					959:976	anxiety/depression	959:976	anxiety/depression	959:976	Treatment with NK33 and/or NK98, which were orally gavaged in mice before or after IS treatment, significantly suppressed the occurrence and development of anxiety/depression, infiltration of Iba1⁺ and LPS⁺/CD11b⁺ cells (activated microglia) into the hippocampus, and corticosterone, IL-6, and LPS levels in the blood.
30979031	6	6	theme	Iba1⁺	995:999	arg1	IL-6					1087:1090	IL-6	1087:1090	IL-6	1087:1090	Treatment with NK33 and/or NK98, which were orally gavaged in mice before or after IS treatment, significantly suppressed the occurrence and development of anxiety/depression, infiltration of Iba1⁺ and LPS⁺/CD11b⁺ cells (activated microglia) into the hippocampus, and corticosterone, IL-6, and LPS levels in the blood.
30979031	6	6	theme	Iba1⁺	995:999	arg1	corticosterone					1071:1084	corticosterone	1071:1084	corticosterone	1071:1084	Treatment with NK33 and/or NK98, which were orally gavaged in mice before or after IS treatment, significantly suppressed the occurrence and development of anxiety/depression, infiltration of Iba1⁺ and LPS⁺/CD11b⁺ cells (activated microglia) into the hippocampus, and corticosterone, IL-6, and LPS levels in the blood.
30979031	6	7	dep	mice	865:868	arg1	before					870:875	before	870:875	before	870:875	Treatment with NK33 and/or NK98, which were orally gavaged in mice before or after IS treatment, significantly suppressed the occurrence and development of anxiety/depression, infiltration of Iba1⁺ and LPS⁺/CD11b⁺ cells (activated microglia) into the hippocampus, and corticosterone, IL-6, and LPS levels in the blood.
30979031	8	8	theme	IL-6	1350:1353	arg1	expression					1355:1364	IL-6 expression	1350:1364	IL-6 expression in the colon	1350:1377	NK33 and/or NK98 treatments suppressed IS-induced colon shortening, myeloperoxidase activity, infiltration of CD11b⁺/CD11c⁺ cells, and IL-6 expression in the colon.
30979031	11	9	theme	immune	1714:1719	arg1	responses					1721:1729	gut immune responses	1710:1729	gut immune responses	1710:1729	In conclusion, NK33 and NK98 synergistically alleviated the occurrence and development of anxiety/depression and colitis through the regulation of gut immune responses and microbiota composition.
30979031	6	10	theme	infiltration	979:990	arg1	occurrence					929:938	the occurrence	925:938	the occurrence	925:938	Treatment with NK33 and/or NK98, which were orally gavaged in mice before or after IS treatment, significantly suppressed the occurrence and development of anxiety/depression, infiltration of Iba1⁺ and LPS⁺/CD11b⁺ cells (activated microglia) into the hippocampus, and corticosterone, IL-6, and LPS levels in the blood.
30979031	6	10	theme	infiltration	979:990	arg1	development					944:954	development	944:954	development of anxiety/depression, infiltration of Iba1⁺ and LPS⁺/CD11b⁺ cells (activated microglia) into the hippocampus, and corticosterone, IL-6, and LPS levels in the blood	944:1119	Treatment with NK33 and/or NK98, which were orally gavaged in mice before or after IS treatment, significantly suppressed the occurrence and development of anxiety/depression, infiltration of Iba1⁺ and LPS⁺/CD11b⁺ cells (activated microglia) into the hippocampus, and corticosterone, IL-6, and LPS levels in the blood.
30979031	2	11	from	effects	426:432	arg1	colitis					495:501	colitis	495:501	colitis	495:501	To understand whether probiotics could simultaneously alleviate anxiety/depression and colitis, we examined their effects on immobilization stress (IS)-induced anxiety/depression and colitis in mice.
30979031	2	11	from	effects	426:432	arg1	anxiety/depression					472:489	anxiety/depression	472:489	anxiety/depression	472:489	To understand whether probiotics could simultaneously alleviate anxiety/depression and colitis, we examined their effects on immobilization stress (IS)-induced anxiety/depression and colitis in mice.
30979031	6	12	from	development	944:954	arg1	blood					1115:1119	the blood	1111:1119	the blood	1111:1119	Treatment with NK33 and/or NK98, which were orally gavaged in mice before or after IS treatment, significantly suppressed the occurrence and development of anxiety/depression, infiltration of Iba1⁺ and LPS⁺/CD11b⁺ cells (activated microglia) into the hippocampus, and corticosterone, IL-6, and LPS levels in the blood.
30979031	4	13	with	Mice	632:635	arg1	colitis					665:671	colitis	665:671	colitis	665:671	Mice with anxiety/depression and colitis were prepared by IS treatment.
30979031	4	13	with	Mice	632:635	arg1	anxiety/depression					642:659	anxiety/depression	642:659	anxiety/depression	642:659	Mice with anxiety/depression and colitis were prepared by IS treatment.
30979031	6	14	from	anxiety/depression	959:976	arg1	blood					1115:1119	the blood	1111:1119	the blood	1111:1119	Treatment with NK33 and/or NK98, which were orally gavaged in mice before or after IS treatment, significantly suppressed the occurrence and development of anxiety/depression, infiltration of Iba1⁺ and LPS⁺/CD11b⁺ cells (activated microglia) into the hippocampus, and corticosterone, IL-6, and LPS levels in the blood.
30979031	7	15	theme	BDNF	1160:1163	arg1	expression					1165:1174	hippocampal BDNF expression	1148:1174	hippocampal BDNF expression	1148:1174	Furthermore, they induced hippocampal BDNF expression while NF-κB activation was suppressed.
30979031	8	16	theme	CD11b⁺/CD11c⁺	1325:1337	arg1	cells					1339:1343	CD11b⁺/CD11c⁺ cells	1325:1343	CD11b⁺/CD11c⁺ cells	1325:1343	NK33 and/or NK98 treatments suppressed IS-induced colon shortening, myeloperoxidase activity, infiltration of CD11b⁺/CD11c⁺ cells, and IL-6 expression in the colon.
30979031	1	17	theme	psychiatric	244:254	arg1	disorders					256:264	psychiatric disorders	244:264	psychiatric disorders	244:264	The gut dysbiosis by stressors such as immobilization deteriorates psychiatric disorders through microbiota-gut-brain axis activation.
30979031	9	18	theme	excessive	1464:1472	arg1	production					1478:1487	excessive LPS production	1464:1487	excessive LPS production	1464:1487	Their treatments also suppressed the IS-induced fecal Proteobacteria population and excessive LPS production.
30979031	6	19	from	occurrence	929:938	arg1	blood					1115:1119	the blood	1111:1119	the blood	1111:1119	Treatment with NK33 and/or NK98, which were orally gavaged in mice before or after IS treatment, significantly suppressed the occurrence and development of anxiety/depression, infiltration of Iba1⁺ and LPS⁺/CD11b⁺ cells (activated microglia) into the hippocampus, and corticosterone, IL-6, and LPS levels in the blood.
30979031	5	20	theme	-induced	782:789	arg1	cells					796:800	lipopolysaccharide (LPS)-induced BV-2 cells	758:800	lipopolysaccharide (LPS)-induced BV-2 cells	758:800	NK33 and NK98 potently suppressed NF-κB activation in lipopolysaccharide (LPS)-induced BV-2 cells.
30979031	8	21	from	activity	1299:1306	arg1	colon					1373:1377	the colon	1369:1377	the colon	1369:1377	NK33 and/or NK98 treatments suppressed IS-induced colon shortening, myeloperoxidase activity, infiltration of CD11b⁺/CD11c⁺ cells, and IL-6 expression in the colon.
30979031	0	22	theme	NK33	61:64	arg1	Effects					28:34	The Preventive and Curative Effects	0:34	The Preventive and Curative Effects of Lactobacillus reuteri NK33 and Bifidobacterium adolescentis NK98 on Immobilization Stress-Induced Anxiety/Depression and Colitis in Mice.	0:175	The Preventive and Curative Effects of Lactobacillus reuteri NK33 and Bifidobacterium adolescentis NK98 on Immobilization Stress-Induced Anxiety/Depression and Colitis in Mice.
30979031	6	23	theme	LPS	1097:1099	arg1	levels					1101:1106	LPS levels	1097:1106	LPS levels in the blood	1097:1119	Treatment with NK33 and/or NK98, which were orally gavaged in mice before or after IS treatment, significantly suppressed the occurrence and development of anxiety/depression, infiltration of Iba1⁺ and LPS⁺/CD11b⁺ cells (activated microglia) into the hippocampus, and corticosterone, IL-6, and LPS levels in the blood.
30979031	6	24	from	levels	1101:1106	arg1	blood					1115:1119	the blood	1111:1119	the blood	1111:1119	Treatment with NK33 and/or NK98, which were orally gavaged in mice before or after IS treatment, significantly suppressed the occurrence and development of anxiety/depression, infiltration of Iba1⁺ and LPS⁺/CD11b⁺ cells (activated microglia) into the hippocampus, and corticosterone, IL-6, and LPS levels in the blood.
30979031	0	25	theme	Bifidobacterium	70:84	arg1	NK98					99:102	Bifidobacterium adolescentis NK98	70:102	Bifidobacterium adolescentis NK98	70:102	The Preventive and Curative Effects of Lactobacillus reuteri NK33 and Bifidobacterium adolescentis NK98 on Immobilization Stress-Induced Anxiety/Depression and Colitis in Mice.
30979031	1	26	theme	gut	181:183	arg1	dysbiosis					185:193	The gut dysbiosis	177:193	The gut dysbiosis by stressors such as immobilization	177:229	The gut dysbiosis by stressors such as immobilization deteriorates psychiatric disorders through microbiota-gut-brain axis activation.
30979031	0	27	theme	NK98	99:102	arg1	Effects					28:34	The Preventive and Curative Effects	0:34	The Preventive and Curative Effects of Lactobacillus reuteri NK33 and Bifidobacterium adolescentis NK98 on Immobilization Stress-Induced Anxiety/Depression and Colitis in Mice.	0:175	The Preventive and Curative Effects of Lactobacillus reuteri NK33 and Bifidobacterium adolescentis NK98 on Immobilization Stress-Induced Anxiety/Depression and Colitis in Mice.
30979031	9	28	theme	IS-induced	1417:1426	arg1	population					1449:1458	the IS-induced fecal Proteobacteria population	1413:1458	the IS-induced fecal Proteobacteria population	1413:1458	Their treatments also suppressed the IS-induced fecal Proteobacteria population and excessive LPS production.
30979031	6	29	from	IL-6	1087:1090	arg1	blood					1115:1119	the blood	1111:1119	the blood	1111:1119	Treatment with NK33 and/or NK98, which were orally gavaged in mice before or after IS treatment, significantly suppressed the occurrence and development of anxiety/depression, infiltration of Iba1⁺ and LPS⁺/CD11b⁺ cells (activated microglia) into the hippocampus, and corticosterone, IL-6, and LPS levels in the blood.
30979031	2	30	dep	stress	452:457	arg1	IS					460:461	IS	460:461	IS	460:461	To understand whether probiotics could simultaneously alleviate anxiety/depression and colitis, we examined their effects on immobilization stress (IS)-induced anxiety/depression and colitis in mice.
30979031	2	31	theme	-induced	463:470	arg1	anxiety/depression					472:489	anxiety/depression	472:489	anxiety/depression	472:489	To understand whether probiotics could simultaneously alleviate anxiety/depression and colitis, we examined their effects on immobilization stress (IS)-induced anxiety/depression and colitis in mice.
30979031	0	32	theme	Immobilization	107:120	arg1	Anxiety/Depression					137:154	Immobilization Stress-Induced Anxiety/Depression	107:154	Immobilization Stress-Induced Anxiety/Depression	107:154	The Preventive and Curative Effects of Lactobacillus reuteri NK33 and Bifidobacterium adolescentis NK98 on Immobilization Stress-Induced Anxiety/Depression and Colitis in Mice.
30979031	9	33	theme	Proteobacteria	1434:1447	arg1	population					1449:1458	the IS-induced fecal Proteobacteria population	1413:1458	the IS-induced fecal Proteobacteria population	1413:1458	Their treatments also suppressed the IS-induced fecal Proteobacteria population and excessive LPS production.
30979031	0	34	from	Effects	28:34	arg1	Colitis					160:166	Colitis	160:166	Colitis in Mice	160:174	The Preventive and Curative Effects of Lactobacillus reuteri NK33 and Bifidobacterium adolescentis NK98 on Immobilization Stress-Induced Anxiety/Depression and Colitis in Mice.
30979031	0	34	from	Effects	28:34	arg1	Anxiety/Depression					137:154	Immobilization Stress-Induced Anxiety/Depression	107:154	Immobilization Stress-Induced Anxiety/Depression	107:154	The Preventive and Curative Effects of Lactobacillus reuteri NK33 and Bifidobacterium adolescentis NK98 on Immobilization Stress-Induced Anxiety/Depression and Colitis in Mice.
30979031	0	35	dep	Bifidobacterium	70:84	arg1	adolescentis					86:97	adolescentis	86:97	adolescentis	86:97	The Preventive and Curative Effects of Lactobacillus reuteri NK33 and Bifidobacterium adolescentis NK98 on Immobilization Stress-Induced Anxiety/Depression and Colitis in Mice.
30979031	6	36	with	Treatment	803:811	arg1	NK98					830:833	NK98	830:833	NK98	830:833	Treatment with NK33 and/or NK98, which were orally gavaged in mice before or after IS treatment, significantly suppressed the occurrence and development of anxiety/depression, infiltration of Iba1⁺ and LPS⁺/CD11b⁺ cells (activated microglia) into the hippocampus, and corticosterone, IL-6, and LPS levels in the blood.
30979031	6	36	with	Treatment	803:811	arg1	NK33					818:821	NK33	818:821	NK33	818:821	Treatment with NK33 and/or NK98, which were orally gavaged in mice before or after IS treatment, significantly suppressed the occurrence and development of anxiety/depression, infiltration of Iba1⁺ and LPS⁺/CD11b⁺ cells (activated microglia) into the hippocampus, and corticosterone, IL-6, and LPS levels in the blood.
30979031	9	37	theme	LPS	1474:1476	arg1	production					1478:1487	excessive LPS production	1464:1487	excessive LPS production	1464:1487	Their treatments also suppressed the IS-induced fecal Proteobacteria population and excessive LPS production.
30979031	6	38	from	corticosterone	1071:1084	arg1	blood					1115:1119	the blood	1111:1119	the blood	1111:1119	Treatment with NK33 and/or NK98, which were orally gavaged in mice before or after IS treatment, significantly suppressed the occurrence and development of anxiety/depression, infiltration of Iba1⁺ and LPS⁺/CD11b⁺ cells (activated microglia) into the hippocampus, and corticosterone, IL-6, and LPS levels in the blood.
30979031	3	39	theme	Lactobacillus	527:539	arg1	probiotics					516:525	The probiotics	512:525	The probiotics Lactobacillus reuteri NK33 and Bifidobacterium adolescentis NK98	512:590	The probiotics Lactobacillus reuteri NK33 and Bifidobacterium adolescentis NK98 were isolated from healthy human feces.
30979031	3	39	theme	Lactobacillus	527:539	arg1	NK33					549:552	Lactobacillus reuteri NK33	527:552	Lactobacillus reuteri NK33	527:552	The probiotics Lactobacillus reuteri NK33 and Bifidobacterium adolescentis NK98 were isolated from healthy human feces.
30979031	0	40	dep	Lactobacillus	39:51	arg1	reuteri					53:59	reuteri	53:59	reuteri	53:59	The Preventive and Curative Effects of Lactobacillus reuteri NK33 and Bifidobacterium adolescentis NK98 on Immobilization Stress-Induced Anxiety/Depression and Colitis in Mice.
30979031	0	41	theme	Lactobacillus	39:51	arg1	NK33					61:64	Lactobacillus reuteri NK33	39:64	Lactobacillus reuteri NK33	39:64	The Preventive and Curative Effects of Lactobacillus reuteri NK33 and Bifidobacterium adolescentis NK98 on Immobilization Stress-Induced Anxiety/Depression and Colitis in Mice.
30979031	3	42	dep	probiotics	516:525	arg1	NK98					587:590	Bifidobacterium adolescentis NK98	558:590	Bifidobacterium adolescentis NK98	558:590	The probiotics Lactobacillus reuteri NK33 and Bifidobacterium adolescentis NK98 were isolated from healthy human feces.
30979031	3	42	dep	probiotics	516:525	arg1	probiotics					516:525	The probiotics	512:525	The probiotics Lactobacillus reuteri NK33 and Bifidobacterium adolescentis NK98	512:590	The probiotics Lactobacillus reuteri NK33 and Bifidobacterium adolescentis NK98 were isolated from healthy human feces.
30979031	3	42	dep	probiotics	516:525	arg1	NK33					549:552	Lactobacillus reuteri NK33	527:552	Lactobacillus reuteri NK33	527:552	The probiotics Lactobacillus reuteri NK33 and Bifidobacterium adolescentis NK98 were isolated from healthy human feces.
30979031	11	43	theme	microbiota	1735:1744	arg1	composition					1746:1756	microbiota composition	1735:1756	microbiota composition	1735:1756	In conclusion, NK33 and NK98 synergistically alleviated the occurrence and development of anxiety/depression and colitis through the regulation of gut immune responses and microbiota composition.
30979031	3	44	theme	Bifidobacterium	558:572	arg1	NK98					587:590	Bifidobacterium adolescentis NK98	558:590	Bifidobacterium adolescentis NK98	558:590	The probiotics Lactobacillus reuteri NK33 and Bifidobacterium adolescentis NK98 were isolated from healthy human feces.
30979031	3	44	theme	Bifidobacterium	558:572	arg1	probiotics					516:525	The probiotics	512:525	The probiotics Lactobacillus reuteri NK33 and Bifidobacterium adolescentis NK98	512:590	The probiotics Lactobacillus reuteri NK33 and Bifidobacterium adolescentis NK98 were isolated from healthy human feces.
30979031	0	45	from	Colitis	160:166	arg1	Mice					171:174	Mice	171:174	Mice	171:174	The Preventive and Curative Effects of Lactobacillus reuteri NK33 and Bifidobacterium adolescentis NK98 on Immobilization Stress-Induced Anxiety/Depression and Colitis in Mice.
30979031	3	46	theme	healthy	611:617	arg1	feces					625:629	healthy human feces	611:629	healthy human feces	611:629	The probiotics Lactobacillus reuteri NK33 and Bifidobacterium adolescentis NK98 were isolated from healthy human feces.
30979031	1	47	theme	microbiota-gut-brain	274:293	arg1	activation					300:309	microbiota-gut-brain axis activation	274:309	microbiota-gut-brain axis activation	274:309	The gut dysbiosis by stressors such as immobilization deteriorates psychiatric disorders through microbiota-gut-brain axis activation.
30979031	8	48	from	infiltration	1309:1320	arg1	colon					1373:1377	the colon	1369:1377	the colon	1369:1377	NK33 and/or NK98 treatments suppressed IS-induced colon shortening, myeloperoxidase activity, infiltration of CD11b⁺/CD11c⁺ cells, and IL-6 expression in the colon.
30979031	0	49	from	Anxiety/Depression	137:154	arg1	Mice					171:174	Mice	171:174	Mice	171:174	The Preventive and Curative Effects of Lactobacillus reuteri NK33 and Bifidobacterium adolescentis NK98 on Immobilization Stress-Induced Anxiety/Depression and Colitis in Mice.
30979031	6	50	theme	cells	1017:1021	arg1	levels					1101:1106	LPS levels	1097:1106	LPS levels in the blood	1097:1119	Treatment with NK33 and/or NK98, which were orally gavaged in mice before or after IS treatment, significantly suppressed the occurrence and development of anxiety/depression, infiltration of Iba1⁺ and LPS⁺/CD11b⁺ cells (activated microglia) into the hippocampus, and corticosterone, IL-6, and LPS levels in the blood.
30979031	6	50	theme	cells	1017:1021	arg1	infiltration					979:990	infiltration	979:990	infiltration of Iba1⁺ and LPS⁺/CD11b⁺ cells (activated microglia) into the hippocampus	979:1064	Treatment with NK33 and/or NK98, which were orally gavaged in mice before or after IS treatment, significantly suppressed the occurrence and development of anxiety/depression, infiltration of Iba1⁺ and LPS⁺/CD11b⁺ cells (activated microglia) into the hippocampus, and corticosterone, IL-6, and LPS levels in the blood.
30979031	6	50	theme	cells	1017:1021	arg1	anxiety/depression					959:976	anxiety/depression	959:976	anxiety/depression	959:976	Treatment with NK33 and/or NK98, which were orally gavaged in mice before or after IS treatment, significantly suppressed the occurrence and development of anxiety/depression, infiltration of Iba1⁺ and LPS⁺/CD11b⁺ cells (activated microglia) into the hippocampus, and corticosterone, IL-6, and LPS levels in the blood.
30979031	6	50	theme	cells	1017:1021	arg1	IL-6					1087:1090	IL-6	1087:1090	IL-6	1087:1090	Treatment with NK33 and/or NK98, which were orally gavaged in mice before or after IS treatment, significantly suppressed the occurrence and development of anxiety/depression, infiltration of Iba1⁺ and LPS⁺/CD11b⁺ cells (activated microglia) into the hippocampus, and corticosterone, IL-6, and LPS levels in the blood.
30979031	6	50	theme	cells	1017:1021	arg1	corticosterone					1071:1084	corticosterone	1071:1084	corticosterone	1071:1084	Treatment with NK33 and/or NK98, which were orally gavaged in mice before or after IS treatment, significantly suppressed the occurrence and development of anxiety/depression, infiltration of Iba1⁺ and LPS⁺/CD11b⁺ cells (activated microglia) into the hippocampus, and corticosterone, IL-6, and LPS levels in the blood.
30979031	6	51	from	blood	1115:1119	arg1	occurrence					929:938	the occurrence	925:938	the occurrence	925:938	Treatment with NK33 and/or NK98, which were orally gavaged in mice before or after IS treatment, significantly suppressed the occurrence and development of anxiety/depression, infiltration of Iba1⁺ and LPS⁺/CD11b⁺ cells (activated microglia) into the hippocampus, and corticosterone, IL-6, and LPS levels in the blood.
30979031	6	51	from	blood	1115:1119	arg1	development					944:954	development	944:954	development of anxiety/depression, infiltration of Iba1⁺ and LPS⁺/CD11b⁺ cells (activated microglia) into the hippocampus, and corticosterone, IL-6, and LPS levels in the blood	944:1119	Treatment with NK33 and/or NK98, which were orally gavaged in mice before or after IS treatment, significantly suppressed the occurrence and development of anxiety/depression, infiltration of Iba1⁺ and LPS⁺/CD11b⁺ cells (activated microglia) into the hippocampus, and corticosterone, IL-6, and LPS levels in the blood.
30979031	8	52	theme	IS-induced	1254:1263	arg1	shortening					1271:1280	IS-induced colon shortening	1254:1280	IS-induced colon shortening	1254:1280	NK33 and/or NK98 treatments suppressed IS-induced colon shortening, myeloperoxidase activity, infiltration of CD11b⁺/CD11c⁺ cells, and IL-6 expression in the colon.
30979031	6	53	dep	before	870:875	arg1	treatment					889:897	treatment	889:897	treatment	889:897	Treatment with NK33 and/or NK98, which were orally gavaged in mice before or after IS treatment, significantly suppressed the occurrence and development of anxiety/depression, infiltration of Iba1⁺ and LPS⁺/CD11b⁺ cells (activated microglia) into the hippocampus, and corticosterone, IL-6, and LPS levels in the blood.
30979031	7	54	theme	NF-κB	1182:1186	arg1	activation					1188:1197	NF-κB activation	1182:1197	NF-κB activation	1182:1197	Furthermore, they induced hippocampal BDNF expression while NF-κB activation was suppressed.
30979031	10	55	theme	BDNF	1508:1511	arg1	expression					1513:1522	BDNF expression	1508:1522	BDNF expression	1508:1522	They also induced BDNF expression in LPS-induced SH-SY5Y cells in vitro.
30979031	5	56	theme	NF-κB	738:742	arg1	activation					744:753	NF-κB activation	738:753	NF-κB activation in lipopolysaccharide (LPS)-induced BV-2 cells	738:800	NK33 and NK98 potently suppressed NF-κB activation in lipopolysaccharide (LPS)-induced BV-2 cells.
30979031	11	57	theme	responses	1721:1729	arg1	regulation					1696:1705	the regulation	1692:1705	the regulation of gut immune responses and microbiota composition	1692:1756	In conclusion, NK33 and NK98 synergistically alleviated the occurrence and development of anxiety/depression and colitis through the regulation of gut immune responses and microbiota composition.
30979031	8	58	theme	myeloperoxidase	1283:1297	arg1	activity					1299:1306	myeloperoxidase activity	1283:1306	myeloperoxidase activity	1283:1306	NK33 and/or NK98 treatments suppressed IS-induced colon shortening, myeloperoxidase activity, infiltration of CD11b⁺/CD11c⁺ cells, and IL-6 expression in the colon.
30979031	6	59	theme	corticosterone	1071:1084	arg1	occurrence					929:938	the occurrence	925:938	the occurrence	925:938	Treatment with NK33 and/or NK98, which were orally gavaged in mice before or after IS treatment, significantly suppressed the occurrence and development of anxiety/depression, infiltration of Iba1⁺ and LPS⁺/CD11b⁺ cells (activated microglia) into the hippocampus, and corticosterone, IL-6, and LPS levels in the blood.
30979031	6	59	theme	corticosterone	1071:1084	arg1	development					944:954	development	944:954	development of anxiety/depression, infiltration of Iba1⁺ and LPS⁺/CD11b⁺ cells (activated microglia) into the hippocampus, and corticosterone, IL-6, and LPS levels in the blood	944:1119	Treatment with NK33 and/or NK98, which were orally gavaged in mice before or after IS treatment, significantly suppressed the occurrence and development of anxiety/depression, infiltration of Iba1⁺ and LPS⁺/CD11b⁺ cells (activated microglia) into the hippocampus, and corticosterone, IL-6, and LPS levels in the blood.
30979031	7	60	theme	hippocampal	1148:1158	arg1	expression					1165:1174	hippocampal BDNF expression	1148:1174	hippocampal BDNF expression	1148:1174	Furthermore, they induced hippocampal BDNF expression while NF-κB activation was suppressed.
30979031	10	61	theme	SH-SY5Y	1539:1545	arg1	cells					1547:1551	LPS-induced SH-SY5Y cells	1527:1551	LPS-induced SH-SY5Y cells	1527:1551	They also induced BDNF expression in LPS-induced SH-SY5Y cells in vitro.
30979031	8	62	from	expression	1355:1364	arg1	colon					1373:1377	the colon	1369:1377	the colon	1369:1377	NK33 and/or NK98 treatments suppressed IS-induced colon shortening, myeloperoxidase activity, infiltration of CD11b⁺/CD11c⁺ cells, and IL-6 expression in the colon.
30979031	8	63	theme	cells	1339:1343	arg1	expression					1355:1364	IL-6 expression	1350:1364	IL-6 expression in the colon	1350:1377	NK33 and/or NK98 treatments suppressed IS-induced colon shortening, myeloperoxidase activity, infiltration of CD11b⁺/CD11c⁺ cells, and IL-6 expression in the colon.
30979031	8	63	theme	cells	1339:1343	arg1	infiltration					1309:1320	infiltration	1309:1320	infiltration of CD11b⁺/CD11c⁺ cells	1309:1343	NK33 and/or NK98 treatments suppressed IS-induced colon shortening, myeloperoxidase activity, infiltration of CD11b⁺/CD11c⁺ cells, and IL-6 expression in the colon.
30979031	8	63	theme	cells	1339:1343	arg1	shortening					1271:1280	IS-induced colon shortening	1254:1280	IS-induced colon shortening	1254:1280	NK33 and/or NK98 treatments suppressed IS-induced colon shortening, myeloperoxidase activity, infiltration of CD11b⁺/CD11c⁺ cells, and IL-6 expression in the colon.
30979031	8	63	theme	cells	1339:1343	arg1	activity					1299:1306	myeloperoxidase activity	1283:1306	myeloperoxidase activity	1283:1306	NK33 and/or NK98 treatments suppressed IS-induced colon shortening, myeloperoxidase activity, infiltration of CD11b⁺/CD11c⁺ cells, and IL-6 expression in the colon.
30979031	11	64	theme	gut	1710:1712	arg1	responses					1721:1729	gut immune responses	1710:1729	gut immune responses	1710:1729	In conclusion, NK33 and NK98 synergistically alleviated the occurrence and development of anxiety/depression and colitis through the regulation of gut immune responses and microbiota composition.
30979031	5	65	theme	BV-2	791:794	arg1	cells					796:800	lipopolysaccharide (LPS)-induced BV-2 cells	758:800	lipopolysaccharide (LPS)-induced BV-2 cells	758:800	NK33 and NK98 potently suppressed NF-κB activation in lipopolysaccharide (LPS)-induced BV-2 cells.
30979031	6	66	theme	levels	1101:1106	arg1	occurrence					929:938	the occurrence	925:938	the occurrence	925:938	Treatment with NK33 and/or NK98, which were orally gavaged in mice before or after IS treatment, significantly suppressed the occurrence and development of anxiety/depression, infiltration of Iba1⁺ and LPS⁺/CD11b⁺ cells (activated microglia) into the hippocampus, and corticosterone, IL-6, and LPS levels in the blood.
30979031	6	66	theme	levels	1101:1106	arg1	development					944:954	development	944:954	development of anxiety/depression, infiltration of Iba1⁺ and LPS⁺/CD11b⁺ cells (activated microglia) into the hippocampus, and corticosterone, IL-6, and LPS levels in the blood	944:1119	Treatment with NK33 and/or NK98, which were orally gavaged in mice before or after IS treatment, significantly suppressed the occurrence and development of anxiety/depression, infiltration of Iba1⁺ and LPS⁺/CD11b⁺ cells (activated microglia) into the hippocampus, and corticosterone, IL-6, and LPS levels in the blood.
30979031	11	67	theme	anxiety/depression	1653:1670	arg1	occurrence					1623:1632	occurrence	1623:1632	occurrence	1623:1632	In conclusion, NK33 and NK98 synergistically alleviated the occurrence and development of anxiety/depression and colitis through the regulation of gut immune responses and microbiota composition.
30979031	11	67	theme	anxiety/depression	1653:1670	arg1	development					1638:1648	development	1638:1648	development	1638:1648	In conclusion, NK33 and NK98 synergistically alleviated the occurrence and development of anxiety/depression and colitis through the regulation of gut immune responses and microbiota composition.
30979031	6	68	theme	IL-6	1087:1090	arg1	occurrence					929:938	the occurrence	925:938	the occurrence	925:938	Treatment with NK33 and/or NK98, which were orally gavaged in mice before or after IS treatment, significantly suppressed the occurrence and development of anxiety/depression, infiltration of Iba1⁺ and LPS⁺/CD11b⁺ cells (activated microglia) into the hippocampus, and corticosterone, IL-6, and LPS levels in the blood.
30979031	6	68	theme	IL-6	1087:1090	arg1	development					944:954	development	944:954	development of anxiety/depression, infiltration of Iba1⁺ and LPS⁺/CD11b⁺ cells (activated microglia) into the hippocampus, and corticosterone, IL-6, and LPS levels in the blood	944:1119	Treatment with NK33 and/or NK98, which were orally gavaged in mice before or after IS treatment, significantly suppressed the occurrence and development of anxiety/depression, infiltration of Iba1⁺ and LPS⁺/CD11b⁺ cells (activated microglia) into the hippocampus, and corticosterone, IL-6, and LPS levels in the blood.
30979031	11	69	dep	occurrence	1623:1632	arg1	the					1619:1621	the	1619:1621	the	1619:1621	In conclusion, NK33 and NK98 synergistically alleviated the occurrence and development of anxiety/depression and colitis through the regulation of gut immune responses and microbiota composition.
30979031	0	70	theme	Stress-Induced	122:135	arg1	Anxiety/Depression					137:154	Immobilization Stress-Induced Anxiety/Depression	107:154	Immobilization Stress-Induced Anxiety/Depression	107:154	The Preventive and Curative Effects of Lactobacillus reuteri NK33 and Bifidobacterium adolescentis NK98 on Immobilization Stress-Induced Anxiety/Depression and Colitis in Mice.
30979031	9	71	theme	fecal	1428:1432	arg1	population					1449:1458	the IS-induced fecal Proteobacteria population	1413:1458	the IS-induced fecal Proteobacteria population	1413:1458	Their treatments also suppressed the IS-induced fecal Proteobacteria population and excessive LPS production.
30979031	0	72	theme	Preventive	4:13	arg1	Effects					28:34	The Preventive and Curative Effects	0:34	The Preventive and Curative Effects of Lactobacillus reuteri NK33 and Bifidobacterium adolescentis NK98 on Immobilization Stress-Induced Anxiety/Depression and Colitis in Mice.	0:175	The Preventive and Curative Effects of Lactobacillus reuteri NK33 and Bifidobacterium adolescentis NK98 on Immobilization Stress-Induced Anxiety/Depression and Colitis in Mice.
30979031	0	73	theme	Curative	19:26	arg1	Effects					28:34	The Preventive and Curative Effects	0:34	The Preventive and Curative Effects of Lactobacillus reuteri NK33 and Bifidobacterium adolescentis NK98 on Immobilization Stress-Induced Anxiety/Depression and Colitis in Mice.	0:175	The Preventive and Curative Effects of Lactobacillus reuteri NK33 and Bifidobacterium adolescentis NK98 on Immobilization Stress-Induced Anxiety/Depression and Colitis in Mice.
30979031	5	74	from	activation	744:753	arg1	cells					796:800	lipopolysaccharide (LPS)-induced BV-2 cells	758:800	lipopolysaccharide (LPS)-induced BV-2 cells	758:800	NK33 and NK98 potently suppressed NF-κB activation in lipopolysaccharide (LPS)-induced BV-2 cells.
30979031	6	75	theme	anxiety/depression	959:976	arg1	occurrence					929:938	the occurrence	925:938	the occurrence	925:938	Treatment with NK33 and/or NK98, which were orally gavaged in mice before or after IS treatment, significantly suppressed the occurrence and development of anxiety/depression, infiltration of Iba1⁺ and LPS⁺/CD11b⁺ cells (activated microglia) into the hippocampus, and corticosterone, IL-6, and LPS levels in the blood.
30979031	6	75	theme	anxiety/depression	959:976	arg1	development					944:954	development	944:954	development of anxiety/depression, infiltration of Iba1⁺ and LPS⁺/CD11b⁺ cells (activated microglia) into the hippocampus, and corticosterone, IL-6, and LPS levels in the blood	944:1119	Treatment with NK33 and/or NK98, which were orally gavaged in mice before or after IS treatment, significantly suppressed the occurrence and development of anxiety/depression, infiltration of Iba1⁺ and LPS⁺/CD11b⁺ cells (activated microglia) into the hippocampus, and corticosterone, IL-6, and LPS levels in the blood.
30979031	11	76	theme	composition	1746:1756	arg1	regulation					1696:1705	the regulation	1692:1705	the regulation of gut immune responses and microbiota composition	1692:1756	In conclusion, NK33 and NK98 synergistically alleviated the occurrence and development of anxiety/depression and colitis through the regulation of gut immune responses and microbiota composition.
30979031	2	77	theme	immobilization	437:450	arg1	stress					452:457	immobilization stress	437:457	immobilization stress (IS)	437:462	To understand whether probiotics could simultaneously alleviate anxiety/depression and colitis, we examined their effects on immobilization stress (IS)-induced anxiety/depression and colitis in mice.
30979031	6	78	theme	activated	1024:1032	arg1	cells					1017:1021	LPS⁺/CD11b⁺ cells	1005:1021	LPS⁺/CD11b⁺ cells (activated microglia)	1005:1043	Treatment with NK33 and/or NK98, which were orally gavaged in mice before or after IS treatment, significantly suppressed the occurrence and development of anxiety/depression, infiltration of Iba1⁺ and LPS⁺/CD11b⁺ cells (activated microglia) into the hippocampus, and corticosterone, IL-6, and LPS levels in the blood.
30979031	6	78	theme	activated	1024:1032	arg1	microglia					1034:1042	activated microglia	1024:1042	activated microglia	1024:1042	Treatment with NK33 and/or NK98, which were orally gavaged in mice before or after IS treatment, significantly suppressed the occurrence and development of anxiety/depression, infiltration of Iba1⁺ and LPS⁺/CD11b⁺ cells (activated microglia) into the hippocampus, and corticosterone, IL-6, and LPS levels in the blood.
30979031	8	79	from	shortening	1271:1280	arg1	colon					1373:1377	the colon	1369:1377	the colon	1369:1377	NK33 and/or NK98 treatments suppressed IS-induced colon shortening, myeloperoxidase activity, infiltration of CD11b⁺/CD11c⁺ cells, and IL-6 expression in the colon.
30979031	3	80	dep	Lactobacillus	527:539	arg1	reuteri					541:547	reuteri	541:547	reuteri	541:547	The probiotics Lactobacillus reuteri NK33 and Bifidobacterium adolescentis NK98 were isolated from healthy human feces.
30979031	3	81	attach	isolated	597:604	arg1	feces					625:629	healthy human feces	611:629	healthy human feces	611:629	The probiotics Lactobacillus reuteri NK33 and Bifidobacterium adolescentis NK98 were isolated from healthy human feces.
30979031	3	81	attach	isolated	597:604	arg2	NK33					549:552	Lactobacillus reuteri NK33	527:552	Lactobacillus reuteri NK33	527:552	The probiotics Lactobacillus reuteri NK33 and Bifidobacterium adolescentis NK98 were isolated from healthy human feces.
30979031	3	81	attach	isolated	597:604	arg2	probiotics					516:525	The probiotics	512:525	The probiotics Lactobacillus reuteri NK33 and Bifidobacterium adolescentis NK98	512:590	The probiotics Lactobacillus reuteri NK33 and Bifidobacterium adolescentis NK98 were isolated from healthy human feces.
30979031	3	81	attach	isolated	597:604	arg2	NK98					587:590	Bifidobacterium adolescentis NK98	558:590	Bifidobacterium adolescentis NK98	558:590	The probiotics Lactobacillus reuteri NK33 and Bifidobacterium adolescentis NK98 were isolated from healthy human feces.
30979031	3	82	theme	adolescentis	574:585	arg1	NK98					587:590	Bifidobacterium adolescentis NK98	558:590	Bifidobacterium adolescentis NK98	558:590	The probiotics Lactobacillus reuteri NK33 and Bifidobacterium adolescentis NK98 were isolated from healthy human feces.
30979031	3	82	theme	adolescentis	574:585	arg1	probiotics					516:525	The probiotics	512:525	The probiotics Lactobacillus reuteri NK33 and Bifidobacterium adolescentis NK98	512:590	The probiotics Lactobacillus reuteri NK33 and Bifidobacterium adolescentis NK98 were isolated from healthy human feces.
30979031	8	83	theme	NK98	1227:1230	arg1	treatments					1232:1241	NK98 treatments	1227:1241	NK98 treatments	1227:1241	NK33 and/or NK98 treatments suppressed IS-induced colon shortening, myeloperoxidase activity, infiltration of CD11b⁺/CD11c⁺ cells, and IL-6 expression in the colon.
30979031	2	84	from	anxiety/depression	472:489	arg1	mice					506:509	mice	506:509	mice	506:509	To understand whether probiotics could simultaneously alleviate anxiety/depression and colitis, we examined their effects on immobilization stress (IS)-induced anxiety/depression and colitis in mice.
30979031	3	85	theme	human	619:623	arg1	feces					625:629	healthy human feces	611:629	healthy human feces	611:629	The probiotics Lactobacillus reuteri NK33 and Bifidobacterium adolescentis NK98 were isolated from healthy human feces.
30979031	1	86	theme	axis	295:298	arg1	activation					300:309	microbiota-gut-brain axis activation	274:309	microbiota-gut-brain axis activation	274:309	The gut dysbiosis by stressors such as immobilization deteriorates psychiatric disorders through microbiota-gut-brain axis activation.
30528862	3	0	theme	fibroblast	762:771	arg1	behavior					782:789	dermal fibroblast fibrotic behavior	755:789	dermal fibroblast fibrotic behavior	755:789	Experimental findings indicate that expression levels of decorin, a small leucine-rich proteoglycan and an endogenous TGF-β inhibitor, can modulate RDEB disease phenotype by contrasting dermal fibroblast fibrotic behavior.
30528862	2	1	dep	disabling	488:496	arg1	secondary					498:506	secondary	498:506	secondary	498:506	Transforming growth factor-β signaling plays a crucial role in determining RDEB fibrotic microenvironment that leads to the development of disabling secondary disease manifestations, including hand and foot deformities.
30528862	1	2	theme	recessive	172:180	arg1	RDEB					216:219	RDEB	216:219	RDEB	216:219	Loss-of-function mutations in the gene encoding type VII collagen underlie recessive dystrophic epidermolysis bullosa (RDEB), a disease characterized by skin and mucosal blistering, impaired wound healing, and diffuse dermal inflammation and fibrosis.
30528862	1	2	theme	recessive	172:180	arg1	bullosa					207:213	recessive dystrophic epidermolysis bullosa	172:213	recessive dystrophic epidermolysis bullosa (RDEB)	172:220	Loss-of-function mutations in the gene encoding type VII collagen underlie recessive dystrophic epidermolysis bullosa (RDEB), a disease characterized by skin and mucosal blistering, impaired wound healing, and diffuse dermal inflammation and fibrosis.
30528862	1	2	theme	recessive	172:180	arg1	disease					225:231	a disease	223:231	a disease characterized by skin and mucosal blistering, impaired wound healing, and diffuse dermal inflammation and fibrosis	223:346	Loss-of-function mutations in the gene encoding type VII collagen underlie recessive dystrophic epidermolysis bullosa (RDEB), a disease characterized by skin and mucosal blistering, impaired wound healing, and diffuse dermal inflammation and fibrosis.
30528862	1	3	theme	mucosal	259:265	arg1	blistering					267:276	mucosal blistering	259:276	mucosal blistering	259:276	Loss-of-function mutations in the gene encoding type VII collagen underlie recessive dystrophic epidermolysis bullosa (RDEB), a disease characterized by skin and mucosal blistering, impaired wound healing, and diffuse dermal inflammation and fibrosis.
30528862	2	4	theme	manifestations	516:529	arg1	development					473:483	the development	469:483	the development of disabling secondary disease manifestations, including hand and foot deformities	469:566	Transforming growth factor-β signaling plays a crucial role in determining RDEB fibrotic microenvironment that leads to the development of disabling secondary disease manifestations, including hand and foot deformities.
30528862	2	5	theme	factor-β	369:376	arg1	signaling					378:386	Transforming growth factor-β signaling	349:386	Transforming growth factor-β signaling	349:386	Transforming growth factor-β signaling plays a crucial role in determining RDEB fibrotic microenvironment that leads to the development of disabling secondary disease manifestations, including hand and foot deformities.
30528862	10	6	theme	RDEB	1654:1657	arg1	course					1667:1672	RDEB disease course	1654:1672	RDEB disease course	1654:1672	More generally, our data indicate that modifying extracellular matrix composition is an option to improve RDEB disease course.
30528862	5	7	theme	digit	1013:1017	arg1	contraction					1019:1029	digit contraction	1013:1029	digit contraction	1013:1029	Overexpressed decorin was able to enhance survival, and to limit digit contraction and the development of paw deformities.
30528862	4	8	theme	human	933:937	arg1	decorin					939:945	human decorin	933:945	human decorin	933:945	In this study, the ability of decorin to modify RDEB course was investigated by systemically treating RDEB mice with a lentivirus expressing human decorin.
30528862	1	9	theme	impaired	279:286	arg1	healing					294:300	impaired wound healing	279:300	impaired wound healing	279:300	Loss-of-function mutations in the gene encoding type VII collagen underlie recessive dystrophic epidermolysis bullosa (RDEB), a disease characterized by skin and mucosal blistering, impaired wound healing, and diffuse dermal inflammation and fibrosis.
30528862	7	10	theme	injured	1255:1261	arg1	skin					1268:1271	the non-chronically injured back skin	1235:1271	the non-chronically injured back skin	1235:1271	Fibrotic traits were strongly reduced in paw skin and also attenuated in the non-chronically injured back skin.
30528862	7	11	theme	Fibrotic	1162:1169	arg1	traits					1171:1176	Fibrotic traits	1162:1176	Fibrotic traits	1162:1176	Fibrotic traits were strongly reduced in paw skin and also attenuated in the non-chronically injured back skin.
30528862	3	12	theme	Experimental	569:580	arg1	findings					582:589	Experimental findings	569:589	Experimental findings	569:589	Experimental findings indicate that expression levels of decorin, a small leucine-rich proteoglycan and an endogenous TGF-β inhibitor, can modulate RDEB disease phenotype by contrasting dermal fibroblast fibrotic behavior.
30528862	1	13	theme	epidermolysis	193:205	arg1	RDEB					216:219	RDEB	216:219	RDEB	216:219	Loss-of-function mutations in the gene encoding type VII collagen underlie recessive dystrophic epidermolysis bullosa (RDEB), a disease characterized by skin and mucosal blistering, impaired wound healing, and diffuse dermal inflammation and fibrosis.
30528862	1	13	theme	epidermolysis	193:205	arg1	bullosa					207:213	recessive dystrophic epidermolysis bullosa	172:213	recessive dystrophic epidermolysis bullosa (RDEB)	172:220	Loss-of-function mutations in the gene encoding type VII collagen underlie recessive dystrophic epidermolysis bullosa (RDEB), a disease characterized by skin and mucosal blistering, impaired wound healing, and diffuse dermal inflammation and fibrosis.
30528862	1	13	theme	epidermolysis	193:205	arg1	disease					225:231	a disease	223:231	a disease characterized by skin and mucosal blistering, impaired wound healing, and diffuse dermal inflammation and fibrosis	223:346	Loss-of-function mutations in the gene encoding type VII collagen underlie recessive dystrophic epidermolysis bullosa (RDEB), a disease characterized by skin and mucosal blistering, impaired wound healing, and diffuse dermal inflammation and fibrosis.
30528862	7	14	theme	paw	1203:1205	arg1	skin					1207:1210	paw skin	1203:1210	paw skin	1203:1210	Fibrotic traits were strongly reduced in paw skin and also attenuated in the non-chronically injured back skin.
30528862	3	15	theme	fibrotic	773:780	arg1	behavior					782:789	dermal fibroblast fibrotic behavior	755:789	dermal fibroblast fibrotic behavior	755:789	Experimental findings indicate that expression levels of decorin, a small leucine-rich proteoglycan and an endogenous TGF-β inhibitor, can modulate RDEB disease phenotype by contrasting dermal fibroblast fibrotic behavior.
30528862	4	16	theme	RDEB	894:897	arg1	mice					899:902	RDEB mice	894:902	RDEB mice	894:902	In this study, the ability of decorin to modify RDEB course was investigated by systemically treating RDEB mice with a lentivirus expressing human decorin.
30528862	4	17	theme	decorin	822:828	arg1	ability					811:817	the ability	807:817	the ability of decorin to modify RDEB course	807:850	In this study, the ability of decorin to modify RDEB course was investigated by systemically treating RDEB mice with a lentivirus expressing human decorin.
30528862	6	18	theme	TGF-β	1134:1138	arg1	activation					1150:1159	TGF-β signaling activation	1134:1159	TGF-β signaling activation	1134:1159	These effects were associated with decreased TGF-β1 levels and TGF-β signaling activation.
30528862	10	19	theme	matrix	1611:1616	arg1	composition					1618:1628	modifying extracellular matrix composition	1587:1628	modifying extracellular matrix composition	1587:1628	More generally, our data indicate that modifying extracellular matrix composition is an option to improve RDEB disease course.
30528862	10	19	theme	matrix	1611:1616	arg1	option					1636:1641	an option to improve RDEB disease course	1633:1672	an option to improve RDEB disease course	1633:1672	More generally, our data indicate that modifying extracellular matrix composition is an option to improve RDEB disease course.
30528862	9	20	theme	TGF-β	1393:1397	arg1	role					1399:1402	TGF-β role	1393:1402	TGF-β role in promoting fibrosis and disease progression in RDEB	1393:1456	Our findings confirm TGF-β role in promoting fibrosis and disease progression in RDEB, and show that decorin counteracts disease manifestations by inhibiting TGF-β activation.
30528862	1	21	theme	Loss-of-function	97:112	arg1	mutations					114:122	Loss-of-function mutations	97:122	Loss-of-function mutations in the gene encoding type VII collagen	97:161	Loss-of-function mutations in the gene encoding type VII collagen underlie recessive dystrophic epidermolysis bullosa (RDEB), a disease characterized by skin and mucosal blistering, impaired wound healing, and diffuse dermal inflammation and fibrosis.
30528862	7	22	theme	non-chronically	1239:1253	arg1	skin					1268:1271	the non-chronically injured back skin	1235:1271	the non-chronically injured back skin	1235:1271	Fibrotic traits were strongly reduced in paw skin and also attenuated in the non-chronically injured back skin.
30528862	1	23	theme	wound	288:292	arg1	healing					294:300	impaired wound healing	279:300	impaired wound healing	279:300	Loss-of-function mutations in the gene encoding type VII collagen underlie recessive dystrophic epidermolysis bullosa (RDEB), a disease characterized by skin and mucosal blistering, impaired wound healing, and diffuse dermal inflammation and fibrosis.
30528862	8	24	theme	pro-inflammatory	1301:1316	arg1	proteins					1318:1325	pro-inflammatory proteins	1301:1325	pro-inflammatory proteins	1301:1325	However, the expression of pro-inflammatory proteins was not decreased in both paw and back skin.
30528862	3	25	theme	decorin	626:632	arg1	levels					616:621	expression levels	605:621	expression levels of decorin, a small leucine-rich proteoglycan and an endogenous TGF-β inhibitor,	605:702	Experimental findings indicate that expression levels of decorin, a small leucine-rich proteoglycan and an endogenous TGF-β inhibitor, can modulate RDEB disease phenotype by contrasting dermal fibroblast fibrotic behavior.
30528862	2	26	theme	crucial	396:402	arg1	role					404:407	a crucial role	394:407	a crucial role	394:407	Transforming growth factor-β signaling plays a crucial role in determining RDEB fibrotic microenvironment that leads to the development of disabling secondary disease manifestations, including hand and foot deformities.
30528862	9	27	theme	disease	1493:1499	arg1	manifestations					1501:1514	disease manifestations	1493:1514	disease manifestations	1493:1514	Our findings confirm TGF-β role in promoting fibrosis and disease progression in RDEB, and show that decorin counteracts disease manifestations by inhibiting TGF-β activation.
30528862	3	28	theme	expression	605:614	arg1	levels					616:621	expression levels	605:621	expression levels of decorin, a small leucine-rich proteoglycan and an endogenous TGF-β inhibitor,	605:702	Experimental findings indicate that expression levels of decorin, a small leucine-rich proteoglycan and an endogenous TGF-β inhibitor, can modulate RDEB disease phenotype by contrasting dermal fibroblast fibrotic behavior.
30528862	6	29	theme	TGF-β1	1116:1121	arg1	levels					1123:1128	decreased TGF-β1 levels	1106:1128	decreased TGF-β1 levels	1106:1128	These effects were associated with decreased TGF-β1 levels and TGF-β signaling activation.
30528862	0	30	theme	disease	20:26	arg1	progression					28:38	disease progression	20:38	disease progression	20:38	Decorin counteracts disease progression in mice with recessive dystrophic epidermolysis bullosa.
30528862	5	31	dep	able	974:977	arg1	limit					1007:1011	limit	1007:1011	to limit digit contraction and the development of paw deformities	1004:1068	Overexpressed decorin was able to enhance survival, and to limit digit contraction and the development of paw deformities.
30528862	5	31	dep	able	974:977	arg1	enhance					982:988	enhance	982:988	to enhance survival	979:997	Overexpressed decorin was able to enhance survival, and to limit digit contraction and the development of paw deformities.
30528862	1	32	theme	dystrophic	182:191	arg1	RDEB					216:219	RDEB	216:219	RDEB	216:219	Loss-of-function mutations in the gene encoding type VII collagen underlie recessive dystrophic epidermolysis bullosa (RDEB), a disease characterized by skin and mucosal blistering, impaired wound healing, and diffuse dermal inflammation and fibrosis.
30528862	1	32	theme	dystrophic	182:191	arg1	bullosa					207:213	recessive dystrophic epidermolysis bullosa	172:213	recessive dystrophic epidermolysis bullosa (RDEB)	172:220	Loss-of-function mutations in the gene encoding type VII collagen underlie recessive dystrophic epidermolysis bullosa (RDEB), a disease characterized by skin and mucosal blistering, impaired wound healing, and diffuse dermal inflammation and fibrosis.
30528862	1	32	theme	dystrophic	182:191	arg1	disease					225:231	a disease	223:231	a disease characterized by skin and mucosal blistering, impaired wound healing, and diffuse dermal inflammation and fibrosis	223:346	Loss-of-function mutations in the gene encoding type VII collagen underlie recessive dystrophic epidermolysis bullosa (RDEB), a disease characterized by skin and mucosal blistering, impaired wound healing, and diffuse dermal inflammation and fibrosis.
30528862	2	33	theme	disabling	488:496	arg1	foot					551:554	foot	551:554	foot	551:554	Transforming growth factor-β signaling plays a crucial role in determining RDEB fibrotic microenvironment that leads to the development of disabling secondary disease manifestations, including hand and foot deformities.
30528862	2	33	theme	disabling	488:496	arg1	manifestations					516:529	disabling secondary disease manifestations	488:529	disabling secondary disease manifestations	488:529	Transforming growth factor-β signaling plays a crucial role in determining RDEB fibrotic microenvironment that leads to the development of disabling secondary disease manifestations, including hand and foot deformities.
30528862	2	33	theme	disabling	488:496	arg1	hand					542:545	hand	542:545	hand	542:545	Transforming growth factor-β signaling plays a crucial role in determining RDEB fibrotic microenvironment that leads to the development of disabling secondary disease manifestations, including hand and foot deformities.
30528862	6	34	theme	decreased	1106:1114	arg1	levels					1123:1128	decreased TGF-β1 levels	1106:1128	decreased TGF-β1 levels	1106:1128	These effects were associated with decreased TGF-β1 levels and TGF-β signaling activation.
30528862	2	35	theme	disease	508:514	arg1	foot					551:554	foot	551:554	foot	551:554	Transforming growth factor-β signaling plays a crucial role in determining RDEB fibrotic microenvironment that leads to the development of disabling secondary disease manifestations, including hand and foot deformities.
30528862	2	35	theme	disease	508:514	arg1	manifestations					516:529	disabling secondary disease manifestations	488:529	disabling secondary disease manifestations	488:529	Transforming growth factor-β signaling plays a crucial role in determining RDEB fibrotic microenvironment that leads to the development of disabling secondary disease manifestations, including hand and foot deformities.
30528862	2	35	theme	disease	508:514	arg1	hand					542:545	hand	542:545	hand	542:545	Transforming growth factor-β signaling plays a crucial role in determining RDEB fibrotic microenvironment that leads to the development of disabling secondary disease manifestations, including hand and foot deformities.
30528862	10	36	theme	disease	1659:1665	arg1	course					1667:1672	RDEB disease course	1654:1672	RDEB disease course	1654:1672	More generally, our data indicate that modifying extracellular matrix composition is an option to improve RDEB disease course.
30528862	3	37	theme	small	637:641	arg1	proteoglycan					656:667	a small leucine-rich proteoglycan	635:667	a small leucine-rich proteoglycan	635:667	Experimental findings indicate that expression levels of decorin, a small leucine-rich proteoglycan and an endogenous TGF-β inhibitor, can modulate RDEB disease phenotype by contrasting dermal fibroblast fibrotic behavior.
30528862	3	37	theme	small	637:641	arg1	decorin					626:632	decorin	626:632	decorin	626:632	Experimental findings indicate that expression levels of decorin, a small leucine-rich proteoglycan and an endogenous TGF-β inhibitor, can modulate RDEB disease phenotype by contrasting dermal fibroblast fibrotic behavior.
30528862	8	38	theme	proteins	1318:1325	arg1	expression					1287:1296	the expression	1283:1296	the expression of pro-inflammatory proteins	1283:1325	However, the expression of pro-inflammatory proteins was not decreased in both paw and back skin.
30528862	2	39	theme	fibrotic	429:436	arg1	microenvironment					438:453	RDEB fibrotic microenvironment	424:453	RDEB fibrotic microenvironment that leads to the development of disabling secondary disease manifestations, including hand and foot deformities	424:566	Transforming growth factor-β signaling plays a crucial role in determining RDEB fibrotic microenvironment that leads to the development of disabling secondary disease manifestations, including hand and foot deformities.
30528862	6	40	theme	signaling	1140:1148	arg1	activation					1150:1159	TGF-β signaling activation	1134:1159	TGF-β signaling activation	1134:1159	These effects were associated with decreased TGF-β1 levels and TGF-β signaling activation.
30528862	1	41	theme	diffuse	307:313	arg1	inflammation					322:333	diffuse dermal inflammation	307:333	diffuse dermal inflammation	307:333	Loss-of-function mutations in the gene encoding type VII collagen underlie recessive dystrophic epidermolysis bullosa (RDEB), a disease characterized by skin and mucosal blistering, impaired wound healing, and diffuse dermal inflammation and fibrosis.
30528862	3	42	theme	TGF-β	687:691	arg1	inhibitor					693:701	an endogenous TGF-β inhibitor	673:701	an endogenous TGF-β inhibitor	673:701	Experimental findings indicate that expression levels of decorin, a small leucine-rich proteoglycan and an endogenous TGF-β inhibitor, can modulate RDEB disease phenotype by contrasting dermal fibroblast fibrotic behavior.
30528862	3	42	theme	TGF-β	687:691	arg1	decorin					626:632	decorin	626:632	decorin	626:632	Experimental findings indicate that expression levels of decorin, a small leucine-rich proteoglycan and an endogenous TGF-β inhibitor, can modulate RDEB disease phenotype by contrasting dermal fibroblast fibrotic behavior.
30528862	3	43	theme	leucine-rich	643:654	arg1	proteoglycan					656:667	a small leucine-rich proteoglycan	635:667	a small leucine-rich proteoglycan	635:667	Experimental findings indicate that expression levels of decorin, a small leucine-rich proteoglycan and an endogenous TGF-β inhibitor, can modulate RDEB disease phenotype by contrasting dermal fibroblast fibrotic behavior.
30528862	3	43	theme	leucine-rich	643:654	arg1	decorin					626:632	decorin	626:632	decorin	626:632	Experimental findings indicate that expression levels of decorin, a small leucine-rich proteoglycan and an endogenous TGF-β inhibitor, can modulate RDEB disease phenotype by contrasting dermal fibroblast fibrotic behavior.
30528862	9	44	from	role	1399:1402	arg1	RDEB					1453:1456	RDEB	1453:1456	RDEB	1453:1456	Our findings confirm TGF-β role in promoting fibrosis and disease progression in RDEB, and show that decorin counteracts disease manifestations by inhibiting TGF-β activation.
30528862	2	45	theme	RDEB	424:427	arg1	microenvironment					438:453	RDEB fibrotic microenvironment	424:453	RDEB fibrotic microenvironment that leads to the development of disabling secondary disease manifestations, including hand and foot deformities	424:566	Transforming growth factor-β signaling plays a crucial role in determining RDEB fibrotic microenvironment that leads to the development of disabling secondary disease manifestations, including hand and foot deformities.
30528862	0	46	theme	recessive	53:61	arg1	bullosa					88:94	recessive dystrophic epidermolysis bullosa	53:94	recessive dystrophic epidermolysis bullosa	53:94	Decorin counteracts disease progression in mice with recessive dystrophic epidermolysis bullosa.
30528862	1	47	theme	dermal	315:320	arg1	inflammation					322:333	diffuse dermal inflammation	307:333	diffuse dermal inflammation	307:333	Loss-of-function mutations in the gene encoding type VII collagen underlie recessive dystrophic epidermolysis bullosa (RDEB), a disease characterized by skin and mucosal blistering, impaired wound healing, and diffuse dermal inflammation and fibrosis.
30528862	10	48	theme	extracellular	1597:1609	arg1	composition					1618:1628	modifying extracellular matrix composition	1587:1628	modifying extracellular matrix composition	1587:1628	More generally, our data indicate that modifying extracellular matrix composition is an option to improve RDEB disease course.
30528862	10	48	theme	extracellular	1597:1609	arg1	option					1636:1641	an option to improve RDEB disease course	1633:1672	an option to improve RDEB disease course	1633:1672	More generally, our data indicate that modifying extracellular matrix composition is an option to improve RDEB disease course.
30528862	2	49	theme	growth	362:367	arg1	signaling					378:386	Transforming growth factor-β signaling	349:386	Transforming growth factor-β signaling	349:386	Transforming growth factor-β signaling plays a crucial role in determining RDEB fibrotic microenvironment that leads to the development of disabling secondary disease manifestations, including hand and foot deformities.
30528862	9	50	theme	disease	1430:1436	arg1	progression					1438:1448	disease progression	1430:1448	disease progression	1430:1448	Our findings confirm TGF-β role in promoting fibrosis and disease progression in RDEB, and show that decorin counteracts disease manifestations by inhibiting TGF-β activation.
30528862	10	51	theme	modifying	1587:1595	arg1	composition					1618:1628	modifying extracellular matrix composition	1587:1628	modifying extracellular matrix composition	1587:1628	More generally, our data indicate that modifying extracellular matrix composition is an option to improve RDEB disease course.
30528862	10	51	theme	modifying	1587:1595	arg1	option					1636:1641	an option to improve RDEB disease course	1633:1672	an option to improve RDEB disease course	1633:1672	More generally, our data indicate that modifying extracellular matrix composition is an option to improve RDEB disease course.
30528862	3	52	theme	disease	722:728	arg1	phenotype					730:738	RDEB disease phenotype	717:738	RDEB disease phenotype	717:738	Experimental findings indicate that expression levels of decorin, a small leucine-rich proteoglycan and an endogenous TGF-β inhibitor, can modulate RDEB disease phenotype by contrasting dermal fibroblast fibrotic behavior.
30528862	2	53	theme	Transforming	349:360	arg1	signaling					378:386	Transforming growth factor-β signaling	349:386	Transforming growth factor-β signaling	349:386	Transforming growth factor-β signaling plays a crucial role in determining RDEB fibrotic microenvironment that leads to the development of disabling secondary disease manifestations, including hand and foot deformities.
30528862	2	54	dep	hand	542:545	arg1	deformities					556:566	deformities	556:566	deformities	556:566	Transforming growth factor-β signaling plays a crucial role in determining RDEB fibrotic microenvironment that leads to the development of disabling secondary disease manifestations, including hand and foot deformities.
30528862	4	55	theme	RDEB	840:843	arg1	course					845:850	RDEB course	840:850	RDEB course	840:850	In this study, the ability of decorin to modify RDEB course was investigated by systemically treating RDEB mice with a lentivirus expressing human decorin.
30528862	1	56	theme	type	145:148	arg1	collagen					154:161	type VII collagen	145:161	type VII collagen	145:161	Loss-of-function mutations in the gene encoding type VII collagen underlie recessive dystrophic epidermolysis bullosa (RDEB), a disease characterized by skin and mucosal blistering, impaired wound healing, and diffuse dermal inflammation and fibrosis.
30528862	3	57	theme	endogenous	676:685	arg1	inhibitor					693:701	an endogenous TGF-β inhibitor	673:701	an endogenous TGF-β inhibitor	673:701	Experimental findings indicate that expression levels of decorin, a small leucine-rich proteoglycan and an endogenous TGF-β inhibitor, can modulate RDEB disease phenotype by contrasting dermal fibroblast fibrotic behavior.
30528862	3	57	theme	endogenous	676:685	arg1	decorin					626:632	decorin	626:632	decorin	626:632	Experimental findings indicate that expression levels of decorin, a small leucine-rich proteoglycan and an endogenous TGF-β inhibitor, can modulate RDEB disease phenotype by contrasting dermal fibroblast fibrotic behavior.
30528862	1	58	theme	VII	150:152	arg1	collagen					154:161	type VII collagen	145:161	type VII collagen	145:161	Loss-of-function mutations in the gene encoding type VII collagen underlie recessive dystrophic epidermolysis bullosa (RDEB), a disease characterized by skin and mucosal blistering, impaired wound healing, and diffuse dermal inflammation and fibrosis.
30528862	0	59	theme	epidermolysis	74:86	arg1	bullosa					88:94	recessive dystrophic epidermolysis bullosa	53:94	recessive dystrophic epidermolysis bullosa	53:94	Decorin counteracts disease progression in mice with recessive dystrophic epidermolysis bullosa.
30528862	3	60	theme	RDEB	717:720	arg1	phenotype					730:738	RDEB disease phenotype	717:738	RDEB disease phenotype	717:738	Experimental findings indicate that expression levels of decorin, a small leucine-rich proteoglycan and an endogenous TGF-β inhibitor, can modulate RDEB disease phenotype by contrasting dermal fibroblast fibrotic behavior.
30528862	5	61	theme	paw	1054:1056	arg1	deformities					1058:1068	paw deformities	1054:1068	paw deformities	1054:1068	Overexpressed decorin was able to enhance survival, and to limit digit contraction and the development of paw deformities.
30528862	9	62	theme	TGF-β	1530:1534	arg1	activation					1536:1545	TGF-β activation	1530:1545	TGF-β activation	1530:1545	Our findings confirm TGF-β role in promoting fibrosis and disease progression in RDEB, and show that decorin counteracts disease manifestations by inhibiting TGF-β activation.
30528862	1	63	from	mutations	114:122	arg1	gene					131:134	the gene	127:134	the gene encoding type VII collagen	127:161	Loss-of-function mutations in the gene encoding type VII collagen underlie recessive dystrophic epidermolysis bullosa (RDEB), a disease characterized by skin and mucosal blistering, impaired wound healing, and diffuse dermal inflammation and fibrosis.
30528862	0	64	theme	dystrophic	63:72	arg1	bullosa					88:94	recessive dystrophic epidermolysis bullosa	53:94	recessive dystrophic epidermolysis bullosa	53:94	Decorin counteracts disease progression in mice with recessive dystrophic epidermolysis bullosa.
30528862	3	65	theme	dermal	755:760	arg1	behavior					782:789	dermal fibroblast fibrotic behavior	755:789	dermal fibroblast fibrotic behavior	755:789	Experimental findings indicate that expression levels of decorin, a small leucine-rich proteoglycan and an endogenous TGF-β inhibitor, can modulate RDEB disease phenotype by contrasting dermal fibroblast fibrotic behavior.
30528862	5	66	theme	deformities	1058:1068	arg1	contraction					1019:1029	digit contraction	1013:1029	digit contraction	1013:1029	Overexpressed decorin was able to enhance survival, and to limit digit contraction and the development of paw deformities.
30528862	5	66	theme	deformities	1058:1068	arg1	development					1039:1049	the development	1035:1049	the development of paw deformities	1035:1068	Overexpressed decorin was able to enhance survival, and to limit digit contraction and the development of paw deformities.
30528862	0	67	with	mice	43:46	arg1	bullosa					88:94	recessive dystrophic epidermolysis bullosa	53:94	recessive dystrophic epidermolysis bullosa	53:94	Decorin counteracts disease progression in mice with recessive dystrophic epidermolysis bullosa.
30528862	5	68	theme	Overexpressed	948:960	arg1	decorin					962:968	Overexpressed decorin	948:968	Overexpressed decorin	948:968	Overexpressed decorin was able to enhance survival, and to limit digit contraction and the development of paw deformities.
30525954	14	0	theme	pHP	1925:1927	arg1	Co-administration					1883:1899	Co-administration	1883:1899	Co-administration of oligosaccharides and pHP	1883:1927	Co-administration of oligosaccharides and pHP can induce immunological tolerance in mice, although tolerance induction was strongest in the animals that were fed oligosaccharides during the entire protocol.
30525954	3	1	dep	plays	637:641	arg1	2					607:607	2	607:607	2	607:607	This study evaluates (1) whether the exposure duration to different prebiotics alongside a partially hydrolysed whey protein (pHP) influences its' effectiveness to prevent allergy development and (2) whether the gut microbiota plays a role in this process.
30525954	5	2	dep	-2	919:920	arg1	GFshort					923:929	GFshort	923:929	GFshort	923:929	Two groups received an oligosaccharide diet only from day -7 until -2 (GFshort and GFAshort) whereas two other groups received their diets from day -15 until 37 (GFlong and GFAlong).
30525954	5	2	dep	-2	919:920	arg1	GFAshort					935:942	GFAshort	935:942	GFAshort	935:942	Two groups received an oligosaccharide diet only from day -7 until -2 (GFshort and GFAshort) whereas two other groups received their diets from day -15 until 37 (GFlong and GFAlong).
30525954	4	3	theme	buffered	790:797	arg1	saline					799:804	phosphate buffered saline	780:804	phosphate buffered saline	780:804	Mice orally sensitised with whey and/or cholera toxin were orally treated for six days before sensitization with phosphate buffered saline, whey or pHP to potentially induce tolerance.
30525954	15	4	theme	microbial	2095:2103	arg1	changes					2105:2111	Some microbial changes	2090:2111	Some microbial changes	2090:2111	Some microbial changes coincided with tolerance induction, however, a specific mechanism could not be determined based on these data.
30525954	12	5	theme	Bacteroidales	1728:1740	arg1	members					1755:1761	Bacteroidales family S24-7 members	1728:1761	Bacteroidales family S24-7 members	1728:1761	However, in the treatment groups, the dominant Lactobacillus species decreased, being replaced by Bacteroidales family S24-7 members.
30525954	14	6	theme	entire	2073:2078	arg1	protocol					2080:2087	the entire protocol	2069:2087	the entire protocol	2069:2087	Co-administration of oligosaccharides and pHP can induce immunological tolerance in mice, although tolerance induction was strongest in the animals that were fed oligosaccharides during the entire protocol.
30525954	1	7	theme	months	261:266	arg1	age					252:254	the age	248:254	the age of 6 months	248:266	Non-breastfed infants at-risk of allergy are recommended to use a hydrolysed formula before the age of 6 months.
30525954	10	8	theme	inducing	1446:1453	arg1	mechanisms					1455:1464	The tolerance inducing mechanisms	1432:1464	The tolerance inducing mechanisms	1432:1464	The tolerance inducing mechanisms seem to vary according to the oligosaccharide-composition.
30525954	8	9	theme	Faecal	1304:1309	arg1	samples					1311:1317	Faecal samples	1304:1317	Faecal samples	1304:1317	Faecal samples were taken at day -15, -8 and 34.
30525954	14	10	theme	oligosaccharides	1904:1919	arg1	Co-administration					1883:1899	Co-administration	1883:1899	Co-administration of oligosaccharides and pHP	1883:1927	Co-administration of oligosaccharides and pHP can induce immunological tolerance in mice, although tolerance induction was strongest in the animals that were fed oligosaccharides during the entire protocol.
30525954	0	11	theme	allergen-specific	96:112	arg1	tolerance					114:122	allergen-specific tolerance	96:122	allergen-specific tolerance	96:122	Mice co-administrated with partially hydrolysed whey proteins and prebiotic fibre mixtures show allergen-specific tolerance and a modulated gut microbiota.
30525954	13	12	theme	GFlong	1846:1851	arg1	groups					1875:1880	the GFlong, GFAshort and GFAlong groups	1842:1880	the GFlong, GFAshort and GFAlong groups	1842:1880	In addition, the relative abundance of Prevotella was significantly higher in the GFlong, GFAshort and GFAlong groups.
30525954	2	13	theme	prebiotics	285:294	arg1	addition					273:280	The addition	269:280	The addition of prebiotics to this formula	269:310	The addition of prebiotics to this formula may reduce the allergy development in these infants, but clinical evidence is still inconclusive.
30525954	3	14	theme	different	468:476	arg1	prebiotics					478:487	different prebiotics	468:487	different prebiotics alongside a partially hydrolysed whey protein (pHP)	468:539	This study evaluates (1) whether the exposure duration to different prebiotics alongside a partially hydrolysed whey protein (pHP) influences its' effectiveness to prevent allergy development and (2) whether the gut microbiota plays a role in this process.
30525954	2	15	from	development	335:345	arg1	infants					356:362	these infants	350:362	these infants	350:362	The addition of prebiotics to this formula may reduce the allergy development in these infants, but clinical evidence is still inconclusive.
30525954	9	16	theme	ear	1418:1420	arg1	swelling					1422:1429	ear swelling	1418:1429	ear swelling	1418:1429	Feeding mice pHP alone during tolerance induction did not reduce ear swelling.
30525954	1	17	theme	allergy	189:195	arg1	at-risk					178:184	Non-breastfed infants at-risk	156:184	Non-breastfed infants at-risk of allergy	156:195	Non-breastfed infants at-risk of allergy are recommended to use a hydrolysed formula before the age of 6 months.
30525954	14	18	theme	tolerance	1982:1990	arg1	induction					1992:2000	tolerance induction	1982:2000	tolerance induction	1982:2000	Co-administration of oligosaccharides and pHP can induce immunological tolerance in mice, although tolerance induction was strongest in the animals that were fed oligosaccharides during the entire protocol.
30525954	13	19	theme	Prevotella	1803:1812	arg1	higher					1832:1837	higher	1832:1837	higher	1832:1837	In addition, the relative abundance of Prevotella was significantly higher in the GFlong, GFAshort and GFAlong groups.
30525954	13	19	theme	Prevotella	1803:1812	arg1	abundance					1790:1798	the relative abundance	1777:1798	the relative abundance of Prevotella	1777:1812	In addition, the relative abundance of Prevotella was significantly higher in the GFlong, GFAshort and GFAlong groups.
30525954	7	20	theme	serum	1221:1225	arg1	protease-1					1243:1252	serum mouse mast cell protease-1	1221:1252	serum mouse mast cell protease-1	1221:1252	At day 37, mice received whey orally and serum mouse mast cell protease-1, SLPI and whey-specific antibodies were assessed.
30525954	0	21	theme	co-administrated	5:20	arg1	Mice					0:3	Mice	0:3	Mice co-administrated with partially hydrolysed whey proteins and prebiotic fibre mixtures	0:89	Mice co-administrated with partially hydrolysed whey proteins and prebiotic fibre mixtures show allergen-specific tolerance and a modulated gut microbiota.
30525954	6	22	theme	whey	1076:1079	arg1	challenge					1081:1089	an intradermal whey challenge	1061:1089	an intradermal whey challenge	1061:1089	On day 35, mice underwent an intradermal whey challenge, and the acute allergic skin response, shock score, and body temperatures were measured.
30525954	6	23	theme	body	1147:1150	arg1	temperatures					1152:1163	body temperatures	1147:1163	body temperatures	1147:1163	On day 35, mice underwent an intradermal whey challenge, and the acute allergic skin response, shock score, and body temperatures were measured.
30525954	4	24	theme	cholera	707:713	arg1	toxin					715:719	cholera toxin	707:719	cholera toxin	707:719	Mice orally sensitised with whey and/or cholera toxin were orally treated for six days before sensitization with phosphate buffered saline, whey or pHP to potentially induce tolerance.
30525954	11	25	theme	skin	1575:1578	arg1	response					1580:1587	the allergic skin response	1562:1587	the allergic skin response	1562:1587	GFshort, GFlong, and GFAlong reduced the allergic skin response, whereas GFAshort was not potent enough.
30525954	6	26	theme	intradermal	1064:1074	arg1	challenge					1081:1089	an intradermal whey challenge	1061:1089	an intradermal whey challenge	1061:1089	On day 35, mice underwent an intradermal whey challenge, and the acute allergic skin response, shock score, and body temperatures were measured.
30525954	2	27	theme	clinical	369:376	arg1	evidence					378:385	clinical evidence	369:385	clinical evidence	369:385	The addition of prebiotics to this formula may reduce the allergy development in these infants, but clinical evidence is still inconclusive.
30525954	7	28	theme	mouse	1227:1231	arg1	protease-1					1243:1252	serum mouse mast cell protease-1	1221:1252	serum mouse mast cell protease-1	1221:1252	At day 37, mice received whey orally and serum mouse mast cell protease-1, SLPI and whey-specific antibodies were assessed.
30525954	0	29	theme	gut	140:142	arg1	microbiota					144:153	a modulated gut microbiota	128:153	a modulated gut microbiota	128:153	Mice co-administrated with partially hydrolysed whey proteins and prebiotic fibre mixtures show allergen-specific tolerance and a modulated gut microbiota.
30525954	7	30	theme	mast	1233:1236	arg1	protease-1					1243:1252	serum mouse mast cell protease-1	1221:1252	serum mouse mast cell protease-1	1221:1252	At day 37, mice received whey orally and serum mouse mast cell protease-1, SLPI and whey-specific antibodies were assessed.
30525954	3	31	dep	evaluates	421:429	arg1	plays					637:641	plays	637:641	plays a role in this process	637:664	This study evaluates (1) whether the exposure duration to different prebiotics alongside a partially hydrolysed whey protein (pHP) influences its' effectiveness to prevent allergy development and (2) whether the gut microbiota plays a role in this process.
30525954	3	31	dep	evaluates	421:429	arg1	influences					541:550	influences	541:550	influences its' effectiveness to prevent allergy development	541:600	This study evaluates (1) whether the exposure duration to different prebiotics alongside a partially hydrolysed whey protein (pHP) influences its' effectiveness to prevent allergy development and (2) whether the gut microbiota plays a role in this process.
30525954	3	32	theme	exposure	447:454	arg1	duration					456:463	the exposure duration	443:463	the exposure duration to different prebiotics alongside a partially hydrolysed whey protein (pHP)	443:539	This study evaluates (1) whether the exposure duration to different prebiotics alongside a partially hydrolysed whey protein (pHP) influences its' effectiveness to prevent allergy development and (2) whether the gut microbiota plays a role in this process.
30525954	12	33	theme	treatment	1646:1654	arg1	groups					1656:1661	the treatment groups	1642:1661	the treatment groups	1642:1661	However, in the treatment groups, the dominant Lactobacillus species decreased, being replaced by Bacteroidales family S24-7 members.
30525954	0	34	theme	modulated	130:138	arg1	microbiota					144:153	a modulated gut microbiota	128:153	a modulated gut microbiota	128:153	Mice co-administrated with partially hydrolysed whey proteins and prebiotic fibre mixtures show allergen-specific tolerance and a modulated gut microbiota.
30525954	3	35	theme	gut	622:624	arg1	microbiota					626:635	the gut microbiota	618:635	the gut microbiota	618:635	This study evaluates (1) whether the exposure duration to different prebiotics alongside a partially hydrolysed whey protein (pHP) influences its' effectiveness to prevent allergy development and (2) whether the gut microbiota plays a role in this process.
30525954	3	36	theme	hydrolysed	511:520	arg1	pHP					536:538	pHP	536:538	pHP	536:538	This study evaluates (1) whether the exposure duration to different prebiotics alongside a partially hydrolysed whey protein (pHP) influences its' effectiveness to prevent allergy development and (2) whether the gut microbiota plays a role in this process.
30525954	3	36	theme	hydrolysed	511:520	arg1	protein					527:533	a partially hydrolysed whey protein	499:533	a partially hydrolysed whey protein (pHP)	499:539	This study evaluates (1) whether the exposure duration to different prebiotics alongside a partially hydrolysed whey protein (pHP) influences its' effectiveness to prevent allergy development and (2) whether the gut microbiota plays a role in this process.
30525954	0	37	theme	whey	48:51	arg1	proteins					53:60	partially hydrolysed whey proteins	27:60	partially hydrolysed whey proteins	27:60	Mice co-administrated with partially hydrolysed whey proteins and prebiotic fibre mixtures show allergen-specific tolerance and a modulated gut microbiota.
30525954	15	38	theme	specific	2160:2167	arg1	mechanism					2169:2177	a specific mechanism	2158:2177	a specific mechanism	2158:2177	Some microbial changes coincided with tolerance induction, however, a specific mechanism could not be determined based on these data.
30525954	3	39	theme	allergy	582:588	arg1	development					590:600	allergy development	582:600	allergy development	582:600	This study evaluates (1) whether the exposure duration to different prebiotics alongside a partially hydrolysed whey protein (pHP) influences its' effectiveness to prevent allergy development and (2) whether the gut microbiota plays a role in this process.
30525954	1	40	theme	hydrolysed	222:231	arg1	formula					233:239	a hydrolysed formula	220:239	a hydrolysed formula	220:239	Non-breastfed infants at-risk of allergy are recommended to use a hydrolysed formula before the age of 6 months.
30525954	0	41	theme	hydrolysed	37:46	arg1	proteins					53:60	partially hydrolysed whey proteins	27:60	partially hydrolysed whey proteins	27:60	Mice co-administrated with partially hydrolysed whey proteins and prebiotic fibre mixtures show allergen-specific tolerance and a modulated gut microbiota.
30525954	12	42	theme	S24-7	1749:1753	arg1	members					1755:1761	Bacteroidales family S24-7 members	1728:1761	Bacteroidales family S24-7 members	1728:1761	However, in the treatment groups, the dominant Lactobacillus species decreased, being replaced by Bacteroidales family S24-7 members.
30525954	4	43	theme	phosphate	780:788	arg1	saline					799:804	phosphate buffered saline	780:804	phosphate buffered saline	780:804	Mice orally sensitised with whey and/or cholera toxin were orally treated for six days before sensitization with phosphate buffered saline, whey or pHP to potentially induce tolerance.
30525954	12	44	theme	Lactobacillus	1677:1689	arg1	species					1691:1697	the dominant Lactobacillus species	1664:1697	the dominant Lactobacillus species	1664:1697	However, in the treatment groups, the dominant Lactobacillus species decreased, being replaced by Bacteroidales family S24-7 members.
30525954	9	45	theme	tolerance	1383:1391	arg1	induction					1393:1401	tolerance induction	1383:1401	tolerance induction	1383:1401	Feeding mice pHP alone during tolerance induction did not reduce ear swelling.
30525954	11	46	theme	allergic	1566:1573	arg1	response					1580:1587	the allergic skin response	1562:1587	the allergic skin response	1562:1587	GFshort, GFlong, and GFAlong reduced the allergic skin response, whereas GFAshort was not potent enough.
30525954	6	47	theme	shock	1130:1134	arg1	score					1136:1140	shock score	1130:1140	shock score	1130:1140	On day 35, mice underwent an intradermal whey challenge, and the acute allergic skin response, shock score, and body temperatures were measured.
30525954	12	48	theme	dominant	1668:1675	arg1	species					1691:1697	the dominant Lactobacillus species	1664:1697	the dominant Lactobacillus species	1664:1697	However, in the treatment groups, the dominant Lactobacillus species decreased, being replaced by Bacteroidales family S24-7 members.
30525954	5	49	theme	oligosaccharide	875:889	arg1	diet					891:894	an oligosaccharide diet	872:894	an oligosaccharide diet	872:894	Two groups received an oligosaccharide diet only from day -7 until -2 (GFshort and GFAshort) whereas two other groups received their diets from day -15 until 37 (GFlong and GFAlong).
30525954	14	50	theme	immunological	1940:1952	arg1	tolerance					1954:1962	immunological tolerance	1940:1962	immunological tolerance	1940:1962	Co-administration of oligosaccharides and pHP can induce immunological tolerance in mice, although tolerance induction was strongest in the animals that were fed oligosaccharides during the entire protocol.
30525954	9	51	dep	Feeding	1353:1359	arg1	mice					1361:1364	mice	1361:1364	mice	1361:1364	Feeding mice pHP alone during tolerance induction did not reduce ear swelling.
30525954	0	52	with	co-administrated	5:20	arg1	mixtures					82:89	prebiotic fibre mixtures	66:89	prebiotic fibre mixtures	66:89	Mice co-administrated with partially hydrolysed whey proteins and prebiotic fibre mixtures show allergen-specific tolerance and a modulated gut microbiota.
30525954	0	52	with	co-administrated	5:20	arg1	proteins					53:60	partially hydrolysed whey proteins	27:60	partially hydrolysed whey proteins	27:60	Mice co-administrated with partially hydrolysed whey proteins and prebiotic fibre mixtures show allergen-specific tolerance and a modulated gut microbiota.
30525954	15	53	theme	tolerance	2128:2136	arg1	induction					2138:2146	tolerance induction	2128:2146	tolerance induction	2128:2146	Some microbial changes coincided with tolerance induction, however, a specific mechanism could not be determined based on these data.
30525954	6	54	theme	acute	1100:1104	arg1	response					1120:1127	the acute allergic skin response	1096:1127	the acute allergic skin response	1096:1127	On day 35, mice underwent an intradermal whey challenge, and the acute allergic skin response, shock score, and body temperatures were measured.
30525954	12	55	theme	family	1742:1747	arg1	members					1755:1761	Bacteroidales family S24-7 members	1728:1761	Bacteroidales family S24-7 members	1728:1761	However, in the treatment groups, the dominant Lactobacillus species decreased, being replaced by Bacteroidales family S24-7 members.
30525954	7	56	theme	cell	1238:1241	arg1	protease-1					1243:1252	serum mouse mast cell protease-1	1221:1252	serum mouse mast cell protease-1	1221:1252	At day 37, mice received whey orally and serum mouse mast cell protease-1, SLPI and whey-specific antibodies were assessed.
30525954	7	57	theme	whey-specific	1264:1276	arg1	antibodies					1278:1287	whey-specific antibodies	1264:1287	whey-specific antibodies	1264:1287	At day 37, mice received whey orally and serum mouse mast cell protease-1, SLPI and whey-specific antibodies were assessed.
30525954	2	58	theme	allergy	327:333	arg1	development					335:345	the allergy development	323:345	the allergy development in these infants	323:362	The addition of prebiotics to this formula may reduce the allergy development in these infants, but clinical evidence is still inconclusive.
30525954	13	59	theme	relative	1781:1788	arg1	higher					1832:1837	higher	1832:1837	higher	1832:1837	In addition, the relative abundance of Prevotella was significantly higher in the GFlong, GFAshort and GFAlong groups.
30525954	13	59	theme	relative	1781:1788	arg1	abundance					1790:1798	the relative abundance	1777:1798	the relative abundance of Prevotella	1777:1812	In addition, the relative abundance of Prevotella was significantly higher in the GFlong, GFAshort and GFAlong groups.
30525954	6	60	theme	skin	1115:1118	arg1	response					1120:1127	the acute allergic skin response	1096:1127	the acute allergic skin response	1096:1127	On day 35, mice underwent an intradermal whey challenge, and the acute allergic skin response, shock score, and body temperatures were measured.
30525954	13	61	theme	GFAshort	1854:1861	arg1	groups					1875:1880	the GFlong, GFAshort and GFAlong groups	1842:1880	the GFlong, GFAshort and GFAlong groups	1842:1880	In addition, the relative abundance of Prevotella was significantly higher in the GFlong, GFAshort and GFAlong groups.
30525954	1	62	theme	infants	170:176	arg1	at-risk					178:184	Non-breastfed infants at-risk	156:184	Non-breastfed infants at-risk of allergy	156:195	Non-breastfed infants at-risk of allergy are recommended to use a hydrolysed formula before the age of 6 months.
30525954	0	63	theme	fibre	76:80	arg1	mixtures					82:89	prebiotic fibre mixtures	66:89	prebiotic fibre mixtures	66:89	Mice co-administrated with partially hydrolysed whey proteins and prebiotic fibre mixtures show allergen-specific tolerance and a modulated gut microbiota.
30525954	6	64	theme	allergic	1106:1113	arg1	response					1120:1127	the acute allergic skin response	1096:1127	the acute allergic skin response	1096:1127	On day 35, mice underwent an intradermal whey challenge, and the acute allergic skin response, shock score, and body temperatures were measured.
30525954	3	65	dep	influences	541:550	arg1	1					432:432	1	432:432	1	432:432	This study evaluates (1) whether the exposure duration to different prebiotics alongside a partially hydrolysed whey protein (pHP) influences its' effectiveness to prevent allergy development and (2) whether the gut microbiota plays a role in this process.
30525954	1	66	theme	Non-breastfed	156:168	arg1	at-risk					178:184	Non-breastfed infants at-risk	156:184	Non-breastfed infants at-risk of allergy	156:195	Non-breastfed infants at-risk of allergy are recommended to use a hydrolysed formula before the age of 6 months.
30525954	5	67	dep	37	1010:1011	arg1	GFAlong					1025:1031	GFAlong	1025:1031	GFAlong	1025:1031	Two groups received an oligosaccharide diet only from day -7 until -2 (GFshort and GFAshort) whereas two other groups received their diets from day -15 until 37 (GFlong and GFAlong).
30525954	5	67	dep	37	1010:1011	arg1	GFlong					1014:1019	GFlong	1014:1019	GFlong	1014:1019	Two groups received an oligosaccharide diet only from day -7 until -2 (GFshort and GFAshort) whereas two other groups received their diets from day -15 until 37 (GFlong and GFAlong).
30525954	0	68	theme	prebiotic	66:74	arg1	mixtures					82:89	prebiotic fibre mixtures	66:89	prebiotic fibre mixtures	66:89	Mice co-administrated with partially hydrolysed whey proteins and prebiotic fibre mixtures show allergen-specific tolerance and a modulated gut microbiota.
30525954	5	69	theme	other	957:961	arg1	groups					963:968	two other groups	953:968	two other groups	953:968	Two groups received an oligosaccharide diet only from day -7 until -2 (GFshort and GFAshort) whereas two other groups received their diets from day -15 until 37 (GFlong and GFAlong).
30525954	13	70	theme	GFAlong	1867:1873	arg1	groups					1875:1880	the GFlong, GFAshort and GFAlong groups	1842:1880	the GFlong, GFAshort and GFAlong groups	1842:1880	In addition, the relative abundance of Prevotella was significantly higher in the GFlong, GFAshort and GFAlong groups.
30525954	3	71	theme	whey	522:525	arg1	pHP					536:538	pHP	536:538	pHP	536:538	This study evaluates (1) whether the exposure duration to different prebiotics alongside a partially hydrolysed whey protein (pHP) influences its' effectiveness to prevent allergy development and (2) whether the gut microbiota plays a role in this process.
30525954	3	71	theme	whey	522:525	arg1	protein					527:533	a partially hydrolysed whey protein	499:533	a partially hydrolysed whey protein (pHP)	499:539	This study evaluates (1) whether the exposure duration to different prebiotics alongside a partially hydrolysed whey protein (pHP) influences its' effectiveness to prevent allergy development and (2) whether the gut microbiota plays a role in this process.
30525954	10	72	theme	tolerance	1436:1444	arg1	mechanisms					1455:1464	The tolerance inducing mechanisms	1432:1464	The tolerance inducing mechanisms	1432:1464	The tolerance inducing mechanisms seem to vary according to the oligosaccharide-composition.
29408268	9	0	theme	vaginal	1992:1998	arg1	application					2000:2010	vaginal application	1992:2010	vaginal application	1992:2010	The technique is also capable of producing fibers at a yield commensurate with practical applicability, hence we believe that the approach shows considerable promise for the development of progesterone dosage forms for vaginal application.
29408268	4	1	theme	drug	936:939	arg1	loading					941:947	initial drug loading	928:947	initial drug loading	928:947	The fabrication parameters such as solvent system, initial drug loading and polymer composition were varied to facilitate optimisation of fiber structure and efficiency of drug incorporation.
29408268	8	2	theme	structural	1738:1747	arg1	properties					1761:1770	suitable structural and release properties	1729:1770	suitable structural and release properties	1729:1770	Overall, the study has shown that pressurized gyration may produce bioadhesive progesterone-loaded nanofibers which have satisfactory loading of a poorly water-soluble drug as well as having suitable structural and release properties.
29408268	3	3	theme	carrier	749:755	arg1	systems					757:763	the carrier systems	745:763	the carrier systems	745:763	Polyethylene oxide and carboxymethyl cellulose were chosen as polymers to develop the carrier systems, based on previous evidence of their yielding mucoadhesive nanofibers using the pressurized gyration technique.
29408268	6	4	theme	nanocrystalline	1314:1328	arg1	state					1330:1334	a nanocrystalline state	1312:1334	a nanocrystalline state	1312:1334	Thermal and spectroscopic analyses indicated that the drug was incorporated in a nanocrystalline state.
29408268	7	5	theme	hours	1531:1535	arg1	period					1521:1526	a period	1519:1526	a period of hours	1519:1535	Release from the drug-loaded fibers indicated comparable rates of progesterone dissolution to that of Cyclogest, a commercially available progesterone pessary, allowing release over a period of hours.
29408268	4	6	theme	solvent	912:918	arg1	system					920:925	solvent system	912:925	solvent system	912:925	The fabrication parameters such as solvent system, initial drug loading and polymer composition were varied to facilitate optimisation of fiber structure and efficiency of drug incorporation.
29408268	7	7	theme	progesterone	1475:1486	arg1	Cyclogest					1439:1447	Cyclogest	1439:1447	Cyclogest	1439:1447	Release from the drug-loaded fibers indicated comparable rates of progesterone dissolution to that of Cyclogest, a commercially available progesterone pessary, allowing release over a period of hours.
29408268	7	7	theme	progesterone	1475:1486	arg1	pessary					1488:1494	a commercially available progesterone pessary	1450:1494	a commercially available progesterone pessary	1450:1494	Release from the drug-loaded fibers indicated comparable rates of progesterone dissolution to that of Cyclogest, a commercially available progesterone pessary, allowing release over a period of hours.
29408268	5	8	theme	surface	1140:1146	arg1	appearance					1148:1157	satisfactory surface appearance	1127:1157	satisfactory surface appearance	1127:1157	Such studies resulted in the formation of nanofibers with satisfactory surface appearance, diameters in the region of 400 nm and loading of up to 25% progesterone.
29408268	3	9	theme	mucoadhesive	811:822	arg1	nanofibers					824:833	their yielding mucoadhesive nanofibers	796:833	their yielding mucoadhesive nanofibers using the pressurized gyration technique	796:874	Polyethylene oxide and carboxymethyl cellulose were chosen as polymers to develop the carrier systems, based on previous evidence of their yielding mucoadhesive nanofibers using the pressurized gyration technique.
29408268	1	10	theme	progesterone	215:226	arg1	applicability					198:210	the applicability	194:210	the applicability of progesterone in preventing preterm birth	194:254	Recent evidence has continued to support the applicability of progesterone in preventing preterm birth, hence the development of an appropriate vaginal delivery system for this drug would be of considerable interest.
29408268	0	11	theme	birth	146:150	arg1	prevention					123:132	the prevention	119:132	the prevention of pre-term birth	119:150	The development of progesterone-loaded nanofibers using pressurized gyration: A novel approach to vaginal delivery for the prevention of pre-term birth.
29408268	9	12	theme	yield	1828:1832	arg1	commensurate					1834:1845	a yield commensurate	1826:1845	a yield commensurate with practical applicability	1826:1874	The technique is also capable of producing fibers at a yield commensurate with practical applicability, hence we believe that the approach shows considerable promise for the development of progesterone dosage forms for vaginal application.
29408268	3	13	theme	pressurized	845:855	arg1	technique					866:874	the pressurized gyration technique	841:874	the pressurized gyration technique	841:874	Polyethylene oxide and carboxymethyl cellulose were chosen as polymers to develop the carrier systems, based on previous evidence of their yielding mucoadhesive nanofibers using the pressurized gyration technique.
29408268	9	14	from	commensurate	1834:1845	arg1	fibers					1816:1821	fibers	1816:1821	fibers at a yield commensurate with practical applicability	1816:1874	The technique is also capable of producing fibers at a yield commensurate with practical applicability, hence we believe that the approach shows considerable promise for the development of progesterone dosage forms for vaginal application.
29408268	5	15	theme	400 nm	1187:1192	arg1	region					1177:1182	the region	1173:1182	the region of 400 nm	1173:1192	Such studies resulted in the formation of nanofibers with satisfactory surface appearance, diameters in the region of 400 nm and loading of up to 25% progesterone.
29408268	9	16	theme	considerable	1918:1929	arg1	promise					1931:1937	considerable promise	1918:1937	considerable promise for the development of progesterone dosage forms for vaginal application	1918:2010	The technique is also capable of producing fibers at a yield commensurate with practical applicability, hence we believe that the approach shows considerable promise for the development of progesterone dosage forms for vaginal application.
29408268	2	17	theme	vaginal	512:518	arg1	insert					520:525	a vaginal insert	510:525	a vaginal insert	510:525	Here, we describe the development of progesterone-loaded bioadhesive nanofibers using pressurized gyration for potential incorporation into a vaginal insert, with a particular view to addressing the challenges of incorporating a poorly water-soluble drug into a hydrophilic nanofiber carrier.
29408268	0	18	theme	novel	80:84	arg1	approach					86:93	A novel approach	78:93	The development of progesterone-loaded nanofibers using pressurized gyration: A novel approach to vaginal delivery for the prevention of pre-term birth.	0:151	The development of progesterone-loaded nanofibers using pressurized gyration: A novel approach to vaginal delivery for the prevention of pre-term birth.
29408268	2	19	theme	water-soluble	606:618	arg1	drug					620:623	a poorly water-soluble drug	597:623	a poorly water-soluble drug	597:623	Here, we describe the development of progesterone-loaded bioadhesive nanofibers using pressurized gyration for potential incorporation into a vaginal insert, with a particular view to addressing the challenges of incorporating a poorly water-soluble drug into a hydrophilic nanofiber carrier.
29408268	8	20	theme	pressurized	1572:1582	arg1	gyration					1584:1591	pressurized gyration	1572:1591	pressurized gyration	1572:1591	Overall, the study has shown that pressurized gyration may produce bioadhesive progesterone-loaded nanofibers which have satisfactory loading of a poorly water-soluble drug as well as having suitable structural and release properties.
29408268	5	21	from	diameters	1160:1168	arg1	region					1177:1182	the region	1173:1182	the region of 400 nm	1173:1192	Such studies resulted in the formation of nanofibers with satisfactory surface appearance, diameters in the region of 400 nm and loading of up to 25% progesterone.
29408268	1	22	theme	considerable	347:358	arg1	interest					360:367	considerable interest	347:367	considerable interest	347:367	Recent evidence has continued to support the applicability of progesterone in preventing preterm birth, hence the development of an appropriate vaginal delivery system for this drug would be of considerable interest.
29408268	4	23	theme	structure	1021:1029	arg1	efficiency					1035:1044	efficiency	1035:1044	efficiency of drug incorporation	1035:1066	The fabrication parameters such as solvent system, initial drug loading and polymer composition were varied to facilitate optimisation of fiber structure and efficiency of drug incorporation.
29408268	4	23	theme	structure	1021:1029	arg1	optimisation					999:1010	optimisation	999:1010	optimisation of fiber structure	999:1029	The fabrication parameters such as solvent system, initial drug loading and polymer composition were varied to facilitate optimisation of fiber structure and efficiency of drug incorporation.
29408268	5	24	dep	%	1217:1217	arg1	25					1215:1216	25	1215:1216	25	1215:1216	Such studies resulted in the formation of nanofibers with satisfactory surface appearance, diameters in the region of 400 nm and loading of up to 25% progesterone.
29408268	5	25	from	appearance	1148:1157	arg1	region					1177:1182	the region	1173:1182	the region of 400 nm	1173:1192	Such studies resulted in the formation of nanofibers with satisfactory surface appearance, diameters in the region of 400 nm and loading of up to 25% progesterone.
29408268	7	26	theme	dissolution	1416:1426	arg1	rates					1394:1398	comparable rates	1383:1398	comparable rates of progesterone dissolution	1383:1426	Release from the drug-loaded fibers indicated comparable rates of progesterone dissolution to that of Cyclogest, a commercially available progesterone pessary, allowing release over a period of hours.
29408268	8	27	theme	bioadhesive	1605:1615	arg1	nanofibers					1637:1646	bioadhesive progesterone-loaded nanofibers	1605:1646	bioadhesive progesterone-loaded nanofibers which have satisfactory loading of a poorly water-soluble drug as well as having suitable structural and release properties	1605:1770	Overall, the study has shown that pressurized gyration may produce bioadhesive progesterone-loaded nanofibers which have satisfactory loading of a poorly water-soluble drug as well as having suitable structural and release properties.
29408268	5	28	with	nanofibers	1111:1120	arg1	appearance					1148:1157	satisfactory surface appearance	1127:1157	satisfactory surface appearance	1127:1157	Such studies resulted in the formation of nanofibers with satisfactory surface appearance, diameters in the region of 400 nm and loading of up to 25% progesterone.
29408268	5	28	with	nanofibers	1111:1120	arg1	loading					1198:1204	loading	1198:1204	loading of up to 25% progesterone	1198:1230	Such studies resulted in the formation of nanofibers with satisfactory surface appearance, diameters in the region of 400 nm and loading of up to 25% progesterone.
29408268	5	28	with	nanofibers	1111:1120	arg1	diameters					1160:1168	diameters	1160:1168	diameters in the region of 400 nm	1160:1192	Such studies resulted in the formation of nanofibers with satisfactory surface appearance, diameters in the region of 400 nm and loading of up to 25% progesterone.
29408268	5	29	theme	progesterone	1219:1230	arg1	appearance					1148:1157	satisfactory surface appearance	1127:1157	satisfactory surface appearance	1127:1157	Such studies resulted in the formation of nanofibers with satisfactory surface appearance, diameters in the region of 400 nm and loading of up to 25% progesterone.
29408268	5	29	theme	progesterone	1219:1230	arg1	loading					1198:1204	loading	1198:1204	loading of up to 25% progesterone	1198:1230	Such studies resulted in the formation of nanofibers with satisfactory surface appearance, diameters in the region of 400 nm and loading of up to 25% progesterone.
29408268	5	29	theme	progesterone	1219:1230	arg1	diameters					1160:1168	diameters	1160:1168	diameters in the region of 400 nm	1160:1192	Such studies resulted in the formation of nanofibers with satisfactory surface appearance, diameters in the region of 400 nm and loading of up to 25% progesterone.
29408268	2	30	theme	bioadhesive	427:437	arg1	nanofibers					439:448	progesterone-loaded bioadhesive nanofibers	407:448	progesterone-loaded bioadhesive nanofibers using pressurized gyration for potential incorporation into a vaginal insert	407:525	Here, we describe the development of progesterone-loaded bioadhesive nanofibers using pressurized gyration for potential incorporation into a vaginal insert, with a particular view to addressing the challenges of incorporating a poorly water-soluble drug into a hydrophilic nanofiber carrier.
29408268	1	31	theme	appropriate	285:295	arg1	system					314:319	an appropriate vaginal delivery system	282:319	an appropriate vaginal delivery system for this drug	282:333	Recent evidence has continued to support the applicability of progesterone in preventing preterm birth, hence the development of an appropriate vaginal delivery system for this drug would be of considerable interest.
29408268	7	32	theme	comparable	1383:1392	arg1	rates					1394:1398	comparable rates	1383:1398	comparable rates of progesterone dissolution	1383:1426	Release from the drug-loaded fibers indicated comparable rates of progesterone dissolution to that of Cyclogest, a commercially available progesterone pessary, allowing release over a period of hours.
29408268	3	33	theme	carboxymethyl	686:698	arg1	polymers					725:732	polymers	725:732	polymers	725:732	Polyethylene oxide and carboxymethyl cellulose were chosen as polymers to develop the carrier systems, based on previous evidence of their yielding mucoadhesive nanofibers using the pressurized gyration technique.
29408268	3	33	theme	carboxymethyl	686:698	arg1	oxide					676:680	Polyethylene oxide	663:680	Polyethylene oxide	663:680	Polyethylene oxide and carboxymethyl cellulose were chosen as polymers to develop the carrier systems, based on previous evidence of their yielding mucoadhesive nanofibers using the pressurized gyration technique.
29408268	3	33	theme	carboxymethyl	686:698	arg1	cellulose					700:708	carboxymethyl cellulose	686:708	carboxymethyl cellulose	686:708	Polyethylene oxide and carboxymethyl cellulose were chosen as polymers to develop the carrier systems, based on previous evidence of their yielding mucoadhesive nanofibers using the pressurized gyration technique.
29408268	0	34	theme	nanofibers	39:48	arg1	development					4:14	The development	0:14	The development of progesterone-loaded nanofibers using pressurized gyration: A novel approach to vaginal delivery for the prevention of pre-term birth.	0:151	The development of progesterone-loaded nanofibers using pressurized gyration: A novel approach to vaginal delivery for the prevention of pre-term birth.
29408268	1	35	theme	delivery	305:312	arg1	system					314:319	an appropriate vaginal delivery system	282:319	an appropriate vaginal delivery system for this drug	282:333	Recent evidence has continued to support the applicability of progesterone in preventing preterm birth, hence the development of an appropriate vaginal delivery system for this drug would be of considerable interest.
29408268	2	36	theme	potential	481:489	arg1	incorporation					491:503	potential incorporation	481:503	potential incorporation into a vaginal insert	481:525	Here, we describe the development of progesterone-loaded bioadhesive nanofibers using pressurized gyration for potential incorporation into a vaginal insert, with a particular view to addressing the challenges of incorporating a poorly water-soluble drug into a hydrophilic nanofiber carrier.
29408268	4	37	theme	fabrication	881:891	arg1	parameters					893:902	The fabrication parameters	877:902	The fabrication parameters such as solvent system, initial drug loading and polymer composition	877:971	The fabrication parameters such as solvent system, initial drug loading and polymer composition were varied to facilitate optimisation of fiber structure and efficiency of drug incorporation.
29408268	4	37	theme	fabrication	881:891	arg1	composition					961:971	polymer composition	953:971	polymer composition	953:971	The fabrication parameters such as solvent system, initial drug loading and polymer composition were varied to facilitate optimisation of fiber structure and efficiency of drug incorporation.
29408268	4	37	theme	fabrication	881:891	arg1	loading					941:947	initial drug loading	928:947	initial drug loading	928:947	The fabrication parameters such as solvent system, initial drug loading and polymer composition were varied to facilitate optimisation of fiber structure and efficiency of drug incorporation.
29408268	4	37	theme	fabrication	881:891	arg1	system					920:925	solvent system	912:925	solvent system	912:925	The fabrication parameters such as solvent system, initial drug loading and polymer composition were varied to facilitate optimisation of fiber structure and efficiency of drug incorporation.
29408268	0	38	theme	pressurized	56:66	arg1	gyration					68:75	pressurized gyration	56:75	pressurized gyration	56:75	The development of progesterone-loaded nanofibers using pressurized gyration: A novel approach to vaginal delivery for the prevention of pre-term birth.
29408268	9	39	theme	dosage	1975:1980	arg1	forms					1982:1986	progesterone dosage forms	1962:1986	progesterone dosage forms	1962:1986	The technique is also capable of producing fibers at a yield commensurate with practical applicability, hence we believe that the approach shows considerable promise for the development of progesterone dosage forms for vaginal application.
29408268	8	40	theme	water-soluble	1692:1704	arg1	drug					1706:1709	a poorly water-soluble drug	1683:1709	a poorly water-soluble drug	1683:1709	Overall, the study has shown that pressurized gyration may produce bioadhesive progesterone-loaded nanofibers which have satisfactory loading of a poorly water-soluble drug as well as having suitable structural and release properties.
29408268	1	41	theme	preterm	242:248	arg1	birth					250:254	preterm birth	242:254	preterm birth	242:254	Recent evidence has continued to support the applicability of progesterone in preventing preterm birth, hence the development of an appropriate vaginal delivery system for this drug would be of considerable interest.
29408268	4	42	theme	drug	1049:1052	arg1	incorporation					1054:1066	drug incorporation	1049:1066	drug incorporation	1049:1066	The fabrication parameters such as solvent system, initial drug loading and polymer composition were varied to facilitate optimisation of fiber structure and efficiency of drug incorporation.
29408268	2	43	theme	nanofiber	644:652	arg1	carrier					654:660	a hydrophilic nanofiber carrier	630:660	a hydrophilic nanofiber carrier	630:660	Here, we describe the development of progesterone-loaded bioadhesive nanofibers using pressurized gyration for potential incorporation into a vaginal insert, with a particular view to addressing the challenges of incorporating a poorly water-soluble drug into a hydrophilic nanofiber carrier.
29408268	4	44	theme	initial	928:934	arg1	loading					941:947	initial drug loading	928:947	initial drug loading	928:947	The fabrication parameters such as solvent system, initial drug loading and polymer composition were varied to facilitate optimisation of fiber structure and efficiency of drug incorporation.
29408268	8	45	theme	suitable	1729:1736	arg1	properties					1761:1770	suitable structural and release properties	1729:1770	suitable structural and release properties	1729:1770	Overall, the study has shown that pressurized gyration may produce bioadhesive progesterone-loaded nanofibers which have satisfactory loading of a poorly water-soluble drug as well as having suitable structural and release properties.
29408268	2	46	theme	hydrophilic	632:642	arg1	carrier					654:660	a hydrophilic nanofiber carrier	630:660	a hydrophilic nanofiber carrier	630:660	Here, we describe the development of progesterone-loaded bioadhesive nanofibers using pressurized gyration for potential incorporation into a vaginal insert, with a particular view to addressing the challenges of incorporating a poorly water-soluble drug into a hydrophilic nanofiber carrier.
29408268	3	47	theme	previous	775:782	arg1	evidence					784:791	previous evidence	775:791	previous evidence of their yielding mucoadhesive nanofibers using the pressurized gyration technique	775:874	Polyethylene oxide and carboxymethyl cellulose were chosen as polymers to develop the carrier systems, based on previous evidence of their yielding mucoadhesive nanofibers using the pressurized gyration technique.
29408268	0	48	theme	pre-term	137:144	arg1	birth					146:150	pre-term birth	137:150	pre-term birth	137:150	The development of progesterone-loaded nanofibers using pressurized gyration: A novel approach to vaginal delivery for the prevention of pre-term birth.
29408268	7	49	theme	available	1465:1473	arg1	Cyclogest					1439:1447	Cyclogest	1439:1447	Cyclogest	1439:1447	Release from the drug-loaded fibers indicated comparable rates of progesterone dissolution to that of Cyclogest, a commercially available progesterone pessary, allowing release over a period of hours.
29408268	7	49	theme	available	1465:1473	arg1	pessary					1488:1494	a commercially available progesterone pessary	1450:1494	a commercially available progesterone pessary	1450:1494	Release from the drug-loaded fibers indicated comparable rates of progesterone dissolution to that of Cyclogest, a commercially available progesterone pessary, allowing release over a period of hours.
29408268	6	50	dep	Thermal	1233:1239	arg1	analyses					1259:1266	analyses	1259:1266	analyses	1259:1266	Thermal and spectroscopic analyses indicated that the drug was incorporated in a nanocrystalline state.
29408268	3	51	theme	nanofibers	824:833	arg1	evidence					784:791	previous evidence	775:791	previous evidence of their yielding mucoadhesive nanofibers using the pressurized gyration technique	775:874	Polyethylene oxide and carboxymethyl cellulose were chosen as polymers to develop the carrier systems, based on previous evidence of their yielding mucoadhesive nanofibers using the pressurized gyration technique.
29408268	3	52	theme	yielding	802:809	arg1	nanofibers					824:833	their yielding mucoadhesive nanofibers	796:833	their yielding mucoadhesive nanofibers using the pressurized gyration technique	796:874	Polyethylene oxide and carboxymethyl cellulose were chosen as polymers to develop the carrier systems, based on previous evidence of their yielding mucoadhesive nanofibers using the pressurized gyration technique.
29408268	5	53	theme	nanofibers	1111:1120	arg1	formation					1098:1106	the formation	1094:1106	the formation of nanofibers with satisfactory surface appearance, diameters in the region of 400 nm and loading of up to 25% progesterone	1094:1230	Such studies resulted in the formation of nanofibers with satisfactory surface appearance, diameters in the region of 400 nm and loading of up to 25% progesterone.
29408268	4	54	theme	polymer	953:959	arg1	composition					961:971	polymer composition	953:971	polymer composition	953:971	The fabrication parameters such as solvent system, initial drug loading and polymer composition were varied to facilitate optimisation of fiber structure and efficiency of drug incorporation.
29408268	8	55	contain	have	1654:1657	arg1	nanofibers					1637:1646	bioadhesive progesterone-loaded nanofibers	1605:1646	bioadhesive progesterone-loaded nanofibers which have satisfactory loading of a poorly water-soluble drug as well as having suitable structural and release properties	1605:1770	Overall, the study has shown that pressurized gyration may produce bioadhesive progesterone-loaded nanofibers which have satisfactory loading of a poorly water-soluble drug as well as having suitable structural and release properties.
29408268	8	55	contain	have	1654:1657	arg2	loading					1672:1678	satisfactory loading	1659:1678	satisfactory loading of a poorly water-soluble drug	1659:1709	Overall, the study has shown that pressurized gyration may produce bioadhesive progesterone-loaded nanofibers which have satisfactory loading of a poorly water-soluble drug as well as having suitable structural and release properties.
29408268	3	56	theme	gyration	857:864	arg1	technique					866:874	the pressurized gyration technique	841:874	the pressurized gyration technique	841:874	Polyethylene oxide and carboxymethyl cellulose were chosen as polymers to develop the carrier systems, based on previous evidence of their yielding mucoadhesive nanofibers using the pressurized gyration technique.
29408268	5	57	theme	satisfactory	1127:1138	arg1	appearance					1148:1157	satisfactory surface appearance	1127:1157	satisfactory surface appearance	1127:1157	Such studies resulted in the formation of nanofibers with satisfactory surface appearance, diameters in the region of 400 nm and loading of up to 25% progesterone.
29408268	9	58	theme	practical	1852:1860	arg1	applicability					1862:1874	practical applicability	1852:1874	practical applicability	1852:1874	The technique is also capable of producing fibers at a yield commensurate with practical applicability, hence we believe that the approach shows considerable promise for the development of progesterone dosage forms for vaginal application.
29408268	1	59	theme	Recent	153:158	arg1	evidence					160:167	Recent evidence	153:167	Recent evidence	153:167	Recent evidence has continued to support the applicability of progesterone in preventing preterm birth, hence the development of an appropriate vaginal delivery system for this drug would be of considerable interest.
29408268	5	60	dep	progesterone	1219:1230	arg1	%					1217:1217	%	1217:1217	%	1217:1217	Such studies resulted in the formation of nanofibers with satisfactory surface appearance, diameters in the region of 400 nm and loading of up to 25% progesterone.
29408268	0	61	theme	vaginal	98:104	arg1	delivery					106:113	vaginal delivery	98:113	vaginal delivery	98:113	The development of progesterone-loaded nanofibers using pressurized gyration: A novel approach to vaginal delivery for the prevention of pre-term birth.
29408268	2	62	theme	particular	535:544	arg1	view					546:549	a particular view	533:549	a particular view to addressing the challenges of incorporating a poorly water-soluble drug into a hydrophilic nanofiber carrier	533:660	Here, we describe the development of progesterone-loaded bioadhesive nanofibers using pressurized gyration for potential incorporation into a vaginal insert, with a particular view to addressing the challenges of incorporating a poorly water-soluble drug into a hydrophilic nanofiber carrier.
29408268	5	63	dep	25	1215:1216	arg1	to					1212:1213	to	1212:1213	to	1212:1213	Such studies resulted in the formation of nanofibers with satisfactory surface appearance, diameters in the region of 400 nm and loading of up to 25% progesterone.
29408268	7	64	theme	progesterone	1403:1414	arg1	dissolution					1416:1426	progesterone dissolution	1403:1426	progesterone dissolution	1403:1426	Release from the drug-loaded fibers indicated comparable rates of progesterone dissolution to that of Cyclogest, a commercially available progesterone pessary, allowing release over a period of hours.
29408268	4	65	theme	fiber	1015:1019	arg1	structure					1021:1029	fiber structure	1015:1029	fiber structure	1015:1029	The fabrication parameters such as solvent system, initial drug loading and polymer composition were varied to facilitate optimisation of fiber structure and efficiency of drug incorporation.
29408268	8	66	theme	progesterone-loaded	1617:1635	arg1	nanofibers					1637:1646	bioadhesive progesterone-loaded nanofibers	1605:1646	bioadhesive progesterone-loaded nanofibers which have satisfactory loading of a poorly water-soluble drug as well as having suitable structural and release properties	1605:1770	Overall, the study has shown that pressurized gyration may produce bioadhesive progesterone-loaded nanofibers which have satisfactory loading of a poorly water-soluble drug as well as having suitable structural and release properties.
29408268	9	67	with	commensurate	1834:1845	arg1	applicability					1862:1874	practical applicability	1852:1874	practical applicability	1852:1874	The technique is also capable of producing fibers at a yield commensurate with practical applicability, hence we believe that the approach shows considerable promise for the development of progesterone dosage forms for vaginal application.
29408268	2	68	theme	pressurized	456:466	arg1	gyration					468:475	pressurized gyration	456:475	pressurized gyration for potential incorporation into a vaginal insert	456:525	Here, we describe the development of progesterone-loaded bioadhesive nanofibers using pressurized gyration for potential incorporation into a vaginal insert, with a particular view to addressing the challenges of incorporating a poorly water-soluble drug into a hydrophilic nanofiber carrier.
29408268	0	69	theme	progesterone-loaded	19:37	arg1	nanofibers					39:48	progesterone-loaded nanofibers	19:48	progesterone-loaded nanofibers using pressurized gyration	19:75	The development of progesterone-loaded nanofibers using pressurized gyration: A novel approach to vaginal delivery for the prevention of pre-term birth.
29408268	0	70	dep	development	4:14	arg1	approach					86:93	A novel approach	78:93	The development of progesterone-loaded nanofibers using pressurized gyration: A novel approach to vaginal delivery for the prevention of pre-term birth.	0:151	The development of progesterone-loaded nanofibers using pressurized gyration: A novel approach to vaginal delivery for the prevention of pre-term birth.
29408268	2	71	theme	nanofibers	439:448	arg1	development					392:402	the development	388:402	the development of progesterone-loaded bioadhesive nanofibers using pressurized gyration for potential incorporation into a vaginal insert	388:525	Here, we describe the development of progesterone-loaded bioadhesive nanofibers using pressurized gyration for potential incorporation into a vaginal insert, with a particular view to addressing the challenges of incorporating a poorly water-soluble drug into a hydrophilic nanofiber carrier.
29408268	8	72	theme	satisfactory	1659:1670	arg1	loading					1672:1678	satisfactory loading	1659:1678	satisfactory loading of a poorly water-soluble drug	1659:1709	Overall, the study has shown that pressurized gyration may produce bioadhesive progesterone-loaded nanofibers which have satisfactory loading of a poorly water-soluble drug as well as having suitable structural and release properties.
29408268	2	73	theme	progesterone-loaded	407:425	arg1	nanofibers					439:448	progesterone-loaded bioadhesive nanofibers	407:448	progesterone-loaded bioadhesive nanofibers using pressurized gyration for potential incorporation into a vaginal insert	407:525	Here, we describe the development of progesterone-loaded bioadhesive nanofibers using pressurized gyration for potential incorporation into a vaginal insert, with a particular view to addressing the challenges of incorporating a poorly water-soluble drug into a hydrophilic nanofiber carrier.
29408268	5	74	from	loading	1198:1204	arg1	region					1177:1182	the region	1173:1182	the region of 400 nm	1173:1192	Such studies resulted in the formation of nanofibers with satisfactory surface appearance, diameters in the region of 400 nm and loading of up to 25% progesterone.
29408268	1	75	theme	vaginal	297:303	arg1	system					314:319	an appropriate vaginal delivery system	282:319	an appropriate vaginal delivery system for this drug	282:333	Recent evidence has continued to support the applicability of progesterone in preventing preterm birth, hence the development of an appropriate vaginal delivery system for this drug would be of considerable interest.
29408268	7	76	theme	drug-loaded	1354:1364	arg1	fibers					1366:1371	the drug-loaded fibers	1350:1371	the drug-loaded fibers	1350:1371	Release from the drug-loaded fibers indicated comparable rates of progesterone dissolution to that of Cyclogest, a commercially available progesterone pessary, allowing release over a period of hours.
29408268	4	77	theme	incorporation	1054:1066	arg1	efficiency					1035:1044	efficiency	1035:1044	efficiency of drug incorporation	1035:1066	The fabrication parameters such as solvent system, initial drug loading and polymer composition were varied to facilitate optimisation of fiber structure and efficiency of drug incorporation.
29408268	4	77	theme	incorporation	1054:1066	arg1	optimisation					999:1010	optimisation	999:1010	optimisation of fiber structure	999:1029	The fabrication parameters such as solvent system, initial drug loading and polymer composition were varied to facilitate optimisation of fiber structure and efficiency of drug incorporation.
29408268	3	78	theme	Polyethylene	663:674	arg1	polymers					725:732	polymers	725:732	polymers	725:732	Polyethylene oxide and carboxymethyl cellulose were chosen as polymers to develop the carrier systems, based on previous evidence of their yielding mucoadhesive nanofibers using the pressurized gyration technique.
29408268	3	78	theme	Polyethylene	663:674	arg1	oxide					676:680	Polyethylene oxide	663:680	Polyethylene oxide	663:680	Polyethylene oxide and carboxymethyl cellulose were chosen as polymers to develop the carrier systems, based on previous evidence of their yielding mucoadhesive nanofibers using the pressurized gyration technique.
29408268	3	78	theme	Polyethylene	663:674	arg1	cellulose					700:708	carboxymethyl cellulose	686:708	carboxymethyl cellulose	686:708	Polyethylene oxide and carboxymethyl cellulose were chosen as polymers to develop the carrier systems, based on previous evidence of their yielding mucoadhesive nanofibers using the pressurized gyration technique.
29408268	1	79	dep	support	186:192	arg1	interest					360:367	considerable interest	347:367	considerable interest	347:367	Recent evidence has continued to support the applicability of progesterone in preventing preterm birth, hence the development of an appropriate vaginal delivery system for this drug would be of considerable interest.
29408268	1	80	theme	system	314:319	arg1	development					267:277	the development	263:277	the development of an appropriate vaginal delivery system for this drug	263:333	Recent evidence has continued to support the applicability of progesterone in preventing preterm birth, hence the development of an appropriate vaginal delivery system for this drug would be of considerable interest.
29408268	7	81	from	fibers	1366:1371	arg1	Release					1337:1343	Release	1337:1343	Release from the drug-loaded fibers	1337:1371	Release from the drug-loaded fibers indicated comparable rates of progesterone dissolution to that of Cyclogest, a commercially available progesterone pessary, allowing release over a period of hours.
29408268	8	82	theme	release	1753:1759	arg1	properties					1761:1770	suitable structural and release properties	1729:1770	suitable structural and release properties	1729:1770	Overall, the study has shown that pressurized gyration may produce bioadhesive progesterone-loaded nanofibers which have satisfactory loading of a poorly water-soluble drug as well as having suitable structural and release properties.
29408268	9	83	theme	progesterone	1962:1973	arg1	forms					1982:1986	progesterone dosage forms	1962:1986	progesterone dosage forms	1962:1986	The technique is also capable of producing fibers at a yield commensurate with practical applicability, hence we believe that the approach shows considerable promise for the development of progesterone dosage forms for vaginal application.
29408268	8	84	theme	drug	1706:1709	arg1	loading					1672:1678	satisfactory loading	1659:1678	satisfactory loading of a poorly water-soluble drug	1659:1709	Overall, the study has shown that pressurized gyration may produce bioadhesive progesterone-loaded nanofibers which have satisfactory loading of a poorly water-soluble drug as well as having suitable structural and release properties.
29408268	5	85	theme	Such	1069:1072	arg1	studies					1074:1080	Such studies	1069:1080	Such studies	1069:1080	Such studies resulted in the formation of nanofibers with satisfactory surface appearance, diameters in the region of 400 nm and loading of up to 25% progesterone.
29408268	9	86	theme	forms	1982:1986	arg1	development					1947:1957	the development	1943:1957	the development of progesterone dosage forms	1943:1986	The technique is also capable of producing fibers at a yield commensurate with practical applicability, hence we believe that the approach shows considerable promise for the development of progesterone dosage forms for vaginal application.
29795787	17	0	theme	association	2507:2517	arg1	map					2496:2498	a first systems-level map	2474:2498	a first systems-level map of the association between microbes and host miRNAs	2474:2550	This work provides a first systems-level map of the association between microbes and host miRNAs in the context of CRC and provides targets for further experimental validation and potential interventions.
29795787	11	1	theme	host	1655:1658	arg1	physiology					1660:1669	host physiology	1655:1669	host physiology	1655:1669	One potential mechanism by which the microbiota can influence host physiology is through affecting gene expression in host cells.
29795787	2	2	theme	CRC	319:321	arg1	progression					323:333	CRC progression	319:333	CRC progression	319:333	MicroRNAs (miRNAs) are known to regulate CRC progression and are associated with patient survival outcomes.
29795787	8	3	theme	CRC-associated	1134:1147	arg1	bacteria					1149:1156	CRC-associated bacteria	1134:1156	CRC-associated bacteria	1134:1156	Additionally, we found that miRNAs that correlated with CRC-associated bacteria are predicted to regulate targets that are relevant for host-microbiome interactions and highlight a possible role for miRNA-driven glycan production in the recruitment of pathogenic microbial taxa.
29795787	16	4	theme	glycan	2354:2359	arg1	production					2361:2370	glycan production	2354:2370	glycan production	2354:2370	Notably, these miRNAs likely regulate glycan production, which is important for the recruitment of pathogenic microbial taxa to the tumor.
29795787	3	5	theme	microbiome	517:526	arg1	composition					494:504	the composition	490:504	the composition of the gut microbiome	490:526	In addition, recent studies suggested that host miRNAs can also regulate bacterial growth and influence the composition of the gut microbiome.
29795787	6	6	theme	DE	844:845	arg1	miRNAs					847:852	These DE miRNAs	838:852	These DE miRNAs	838:852	These DE miRNAs were correlated with the relative abundances of several bacterial taxa, including Firmicutes, Bacteroidetes, and Proteobacteria.
29795787	10	7	theme	gut	1577:1579	arg1	microbiota					1581:1590	the gut microbiota	1573:1590	the gut microbiota	1573:1590	IMPORTANCE Recent studies have found an association between colorectal cancer (CRC) and the gut microbiota.
29795787	9	8	theme	human	1466:1470	arg1	tissues					1476:1482	human CRC tissues	1466:1482	human CRC tissues	1466:1482	Our work characterized a global relationship between microbial community composition and miRNA expression in human CRC tissues.
29795787	12	9	theme	small	1746:1750	arg1	molecules					1766:1774	small noncoding RNA molecules	1746:1774	small noncoding RNA molecules that can regulate gene expression and have important roles in cancer development	1746:1855	MicroRNAs (miRNAs) are small noncoding RNA molecules that can regulate gene expression and have important roles in cancer development.
29795787	12	9	theme	small	1746:1750	arg1	MicroRNAs					1723:1731	MicroRNAs	1723:1731	MicroRNAs (miRNAs)	1723:1740	MicroRNAs (miRNAs) are small noncoding RNA molecules that can regulate gene expression and have important roles in cancer development.
29795787	12	10	contain	have	1814:1817	arg1	molecules					1766:1774	small noncoding RNA molecules	1746:1774	small noncoding RNA molecules that can regulate gene expression and have important roles in cancer development	1746:1855	MicroRNAs (miRNAs) are small noncoding RNA molecules that can regulate gene expression and have important roles in cancer development.
29795787	12	10	contain	have	1814:1817	arg1	MicroRNAs					1723:1731	MicroRNAs	1723:1731	MicroRNAs (miRNAs)	1723:1740	MicroRNAs (miRNAs) are small noncoding RNA molecules that can regulate gene expression and have important roles in cancer development.
29795787	12	10	contain	have	1814:1817	arg2	roles					1829:1833	important roles	1819:1833	important roles	1819:1833	MicroRNAs (miRNAs) are small noncoding RNA molecules that can regulate gene expression and have important roles in cancer development.
29795787	7	11	theme	metabolic	1056:1064	arg1	categories					1066:1075	distinct predicted metabolic categories	1037:1075	distinct predicted metabolic categories	1037:1075	Bacteria correlated with DE miRNAs were enriched with distinct predicted metabolic categories.
29795787	17	12	theme	potential	2635:2643	arg1	interventions					2645:2657	potential interventions	2635:2657	potential interventions	2635:2657	This work provides a first systems-level map of the association between microbes and host miRNAs in the context of CRC and provides targets for further experimental validation and potential interventions.
29795787	12	13	theme	RNA	1762:1764	arg1	molecules					1766:1774	small noncoding RNA molecules	1746:1774	small noncoding RNA molecules that can regulate gene expression and have important roles in cancer development	1746:1855	MicroRNAs (miRNAs) are small noncoding RNA molecules that can regulate gene expression and have important roles in cancer development.
29795787	12	13	theme	RNA	1762:1764	arg1	MicroRNAs					1723:1731	MicroRNAs	1723:1731	MicroRNAs (miRNAs)	1723:1740	MicroRNAs (miRNAs) are small noncoding RNA molecules that can regulate gene expression and have important roles in cancer development.
29795787	8	14	theme	possible	1259:1266	arg1	role					1268:1271	a possible role	1257:1271	a possible role for miRNA-driven glycan production in the recruitment of pathogenic microbial taxa	1257:1354	Additionally, we found that miRNAs that correlated with CRC-associated bacteria are predicted to regulate targets that are relevant for host-microbiome interactions and highlight a possible role for miRNA-driven glycan production in the recruitment of pathogenic microbial taxa.
29795787	7	15	theme	distinct	1037:1044	arg1	categories					1066:1075	distinct predicted metabolic categories	1037:1075	distinct predicted metabolic categories	1037:1075	Bacteria correlated with DE miRNAs were enriched with distinct predicted metabolic categories.
29795787	14	16	theme	miRNAs	1976:1981	arg1	dozens					1966:1971	dozens	1966:1971	dozens of miRNAs	1966:1981	We found that dozens of miRNAs are differentially regulated in CRC tumors and adjacent normal colon and that these miRNAs are correlated with the abundance of microbes in the tumor microenvironment.
29795787	6	17	theme	bacterial	910:918	arg1	Bacteroidetes					948:960	Bacteroidetes	948:960	Bacteroidetes	948:960	These DE miRNAs were correlated with the relative abundances of several bacterial taxa, including Firmicutes, Bacteroidetes, and Proteobacteria.
29795787	6	17	theme	bacterial	910:918	arg1	taxa					920:923	several bacterial taxa	902:923	several bacterial taxa	902:923	These DE miRNAs were correlated with the relative abundances of several bacterial taxa, including Firmicutes, Bacteroidetes, and Proteobacteria.
29795787	6	17	theme	bacterial	910:918	arg1	Firmicutes					936:945	Firmicutes	936:945	Firmicutes	936:945	These DE miRNAs were correlated with the relative abundances of several bacterial taxa, including Firmicutes, Bacteroidetes, and Proteobacteria.
29795787	6	17	theme	bacterial	910:918	arg1	Proteobacteria					967:980	Proteobacteria	967:980	Proteobacteria	967:980	These DE miRNAs were correlated with the relative abundances of several bacterial taxa, including Firmicutes, Bacteroidetes, and Proteobacteria.
29795787	4	18	theme	CRC	628:630	arg1	tumor					632:636	human CRC tumor and normal tissues	622:655	tumor	632:636	Here, we investigated the association between miRNA expression and microbiome composition in human CRC tumor and normal tissues.
29795787	8	19	theme	glycan	1290:1295	arg1	production					1297:1306	miRNA-driven glycan production	1277:1306	miRNA-driven glycan production	1277:1306	Additionally, we found that miRNAs that correlated with CRC-associated bacteria are predicted to regulate targets that are relevant for host-microbiome interactions and highlight a possible role for miRNA-driven glycan production in the recruitment of pathogenic microbial taxa.
29795787	14	20	theme	tumor	2127:2131	arg1	microenvironment					2133:2148	the tumor microenvironment	2123:2148	the tumor microenvironment	2123:2148	We found that dozens of miRNAs are differentially regulated in CRC tumors and adjacent normal colon and that these miRNAs are correlated with the abundance of microbes in the tumor microenvironment.
29795787	6	21	theme	relative	879:886	arg1	abundances					888:897	the relative abundances	875:897	the relative abundances of several bacterial taxa, including Firmicutes, Bacteroidetes, and Proteobacteria	875:980	These DE miRNAs were correlated with the relative abundances of several bacterial taxa, including Firmicutes, Bacteroidetes, and Proteobacteria.
29795787	17	22	theme	experimental	2607:2618	arg1	validation					2620:2629	further experimental validation	2599:2629	further experimental validation	2599:2629	This work provides a first systems-level map of the association between microbes and host miRNAs in the context of CRC and provides targets for further experimental validation and potential interventions.
29795787	13	23	theme	miRNA	1938:1942	arg1	microbiota					1905:1914	the gut microbiota	1897:1914	the gut microbiota	1897:1914	Here, we investigated the link between the gut microbiota and the expression of miRNA in CRC.
29795787	13	23	theme	miRNA	1938:1942	arg1	expression					1924:1933	the expression	1920:1933	the expression of miRNA in CRC	1920:1949	Here, we investigated the link between the gut microbiota and the expression of miRNA in CRC.
29795787	5	24	from	tissue	752:757	arg1	tissue					718:723	tissue	718:723	tissue from CRC tumors and normal tissue	718:757	We identified 76 miRNAs as differentially expressed (DE) in tissue from CRC tumors and normal tissue, including the known oncogenic miRNAs miR-182, miR-503, and mir-17~92 cluster.
29795787	5	25	theme	oncogenic	780:788	arg1	miRNAs					790:795	the known oncogenic miRNAs	770:795	the known oncogenic miRNAs miR-182, miR-503, and mir-17~92 cluster	770:835	We identified 76 miRNAs as differentially expressed (DE) in tissue from CRC tumors and normal tissue, including the known oncogenic miRNAs miR-182, miR-503, and mir-17~92 cluster.
29795787	8	26	theme	pathogenic	1330:1339	arg1	taxa					1351:1354	pathogenic microbial taxa	1330:1354	pathogenic microbial taxa	1330:1354	Additionally, we found that miRNAs that correlated with CRC-associated bacteria are predicted to regulate targets that are relevant for host-microbiome interactions and highlight a possible role for miRNA-driven glycan production in the recruitment of pathogenic microbial taxa.
29795787	13	27	theme	gut	1901:1903	arg1	microbiota					1905:1914	the gut microbiota	1897:1914	the gut microbiota	1897:1914	Here, we investigated the link between the gut microbiota and the expression of miRNA in CRC.
29795787	14	28	theme	microbes	2111:2118	arg1	abundance					2098:2106	the abundance	2094:2106	the abundance of microbes in the tumor microenvironment	2094:2148	We found that dozens of miRNAs are differentially regulated in CRC tumors and adjacent normal colon and that these miRNAs are correlated with the abundance of microbes in the tumor microenvironment.
29795787	17	29	theme	host	2540:2543	arg1	miRNAs					2545:2550	host miRNAs	2540:2550	host miRNAs	2540:2550	This work provides a first systems-level map of the association between microbes and host miRNAs in the context of CRC and provides targets for further experimental validation and potential interventions.
29795787	8	30	theme	taxa	1351:1354	arg1	recruitment					1315:1325	the recruitment	1311:1325	the recruitment of pathogenic microbial taxa	1311:1354	Additionally, we found that miRNAs that correlated with CRC-associated bacteria are predicted to regulate targets that are relevant for host-microbiome interactions and highlight a possible role for miRNA-driven glycan production in the recruitment of pathogenic microbial taxa.
29795787	1	31	theme	gut	102:104	arg1	composition					117:127	gut microbiome composition	102:127	gut microbiome composition	102:127	Although variation in gut microbiome composition has been linked with colorectal cancer (CRC), the factors that mediate the interactions between CRC tumors and the microbiome are poorly understood.
29795787	2	32	theme	survival	367:374	arg1	outcomes					376:383	patient survival outcomes	359:383	patient survival outcomes	359:383	MicroRNAs (miRNAs) are known to regulate CRC progression and are associated with patient survival outcomes.
29795787	10	33	theme	IMPORTANCE	1485:1494	arg1	studies					1503:1509	IMPORTANCE Recent studies	1485:1509	IMPORTANCE Recent studies	1485:1509	IMPORTANCE Recent studies have found an association between colorectal cancer (CRC) and the gut microbiota.
29795787	11	34	from	expression	1697:1706	arg1	cells					1716:1720	host cells	1711:1720	host cells	1711:1720	One potential mechanism by which the microbiota can influence host physiology is through affecting gene expression in host cells.
29795787	17	35	theme	CRC	2570:2572	arg1	context					2559:2565	the context	2555:2565	the context of CRC	2555:2572	This work provides a first systems-level map of the association between microbes and host miRNAs in the context of CRC and provides targets for further experimental validation and potential interventions.
29795787	0	36	theme	Host	20:23	arg1	MicroRNAs					25:33	Host MicroRNAs	20:33	Host MicroRNAs	20:33	Interaction between Host MicroRNAs and the Gut Microbiota in Colorectal Cancer.
29795787	9	37	from	expression	1452:1461	arg1	tissues					1476:1482	human CRC tissues	1466:1482	human CRC tissues	1466:1482	Our work characterized a global relationship between microbial community composition and miRNA expression in human CRC tissues.
29795787	13	38	from	expression	1924:1933	arg1	CRC					1947:1949	CRC	1947:1949	CRC	1947:1949	Here, we investigated the link between the gut microbiota and the expression of miRNA in CRC.
29795787	5	39	theme	normal	745:750	arg1	tissue					752:757	normal tissue	745:757	normal tissue	745:757	We identified 76 miRNAs as differentially expressed (DE) in tissue from CRC tumors and normal tissue, including the known oncogenic miRNAs miR-182, miR-503, and mir-17~92 cluster.
29795787	15	40	with	interactions	2288:2299	arg1	microbes					2306:2313	microbes	2306:2313	microbes	2306:2313	Moreover, we found that microbes that have been previously associated with CRC are correlated with miRNAs that regulate genes related to interactions with microbes.
29795787	3	41	theme	bacterial	459:467	arg1	growth					469:474	bacterial growth	459:474	bacterial growth	459:474	In addition, recent studies suggested that host miRNAs can also regulate bacterial growth and influence the composition of the gut microbiome.
29795787	0	42	theme	Gut	43:45	arg1	Microbiota					47:56	the Gut Microbiota	39:56	the Gut Microbiota in Colorectal Cancer	39:77	Interaction between Host MicroRNAs and the Gut Microbiota in Colorectal Cancer.
29795787	17	43	theme	first	2476:2480	arg1	map					2496:2498	a first systems-level map	2474:2498	a first systems-level map of the association between microbes and host miRNAs	2474:2550	This work provides a first systems-level map of the association between microbes and host miRNAs in the context of CRC and provides targets for further experimental validation and potential interventions.
29795787	14	44	theme	normal	2039:2044	arg1	colon					2046:2050	adjacent normal colon	2030:2050	adjacent normal colon	2030:2050	We found that dozens of miRNAs are differentially regulated in CRC tumors and adjacent normal colon and that these miRNAs are correlated with the abundance of microbes in the tumor microenvironment.
29795787	4	45	theme	miRNA	575:579	arg1	expression					581:590	miRNA expression	575:590	miRNA expression	575:590	Here, we investigated the association between miRNA expression and microbiome composition in human CRC tumor and normal tissues.
29795787	5	46	dep	miRNAs	790:795	arg1	cluster					829:835	miR-182, miR-503, and mir-17~92 cluster	797:835	the known oncogenic miRNAs miR-182, miR-503, and mir-17~92 cluster	770:835	We identified 76 miRNAs as differentially expressed (DE) in tissue from CRC tumors and normal tissue, including the known oncogenic miRNAs miR-182, miR-503, and mir-17~92 cluster.
29795787	1	47	from	variation	89:97	arg1	composition					117:127	gut microbiome composition	102:127	gut microbiome composition	102:127	Although variation in gut microbiome composition has been linked with colorectal cancer (CRC), the factors that mediate the interactions between CRC tumors and the microbiome are poorly understood.
29795787	9	48	theme	microbial	1410:1418	arg1	composition					1430:1440	microbial community composition	1410:1440	microbial community composition	1410:1440	Our work characterized a global relationship between microbial community composition and miRNA expression in human CRC tissues.
29795787	12	49	theme	important	1819:1827	arg1	roles					1829:1833	important roles	1819:1833	important roles	1819:1833	MicroRNAs (miRNAs) are small noncoding RNA molecules that can regulate gene expression and have important roles in cancer development.
29795787	5	50	theme	CRC	730:732	arg1	tumors					734:739	CRC tumors	730:739	CRC tumors	730:739	We identified 76 miRNAs as differentially expressed (DE) in tissue from CRC tumors and normal tissue, including the known oncogenic miRNAs miR-182, miR-503, and mir-17~92 cluster.
29795787	16	51	theme	microbial	2426:2434	arg1	taxa					2436:2439	pathogenic microbial taxa	2415:2439	pathogenic microbial taxa	2415:2439	Notably, these miRNAs likely regulate glycan production, which is important for the recruitment of pathogenic microbial taxa to the tumor.
29795787	3	52	theme	gut	513:515	arg1	microbiome					517:526	the gut microbiome	509:526	the gut microbiome	509:526	In addition, recent studies suggested that host miRNAs can also regulate bacterial growth and influence the composition of the gut microbiome.
29795787	9	53	theme	miRNA	1446:1450	arg1	expression					1452:1461	miRNA expression	1446:1461	miRNA expression	1446:1461	Our work characterized a global relationship between microbial community composition and miRNA expression in human CRC tissues.
29795787	12	54	theme	gene	1794:1797	arg1	expression					1799:1808	gene expression	1794:1808	gene expression	1794:1808	MicroRNAs (miRNAs) are small noncoding RNA molecules that can regulate gene expression and have important roles in cancer development.
29795787	14	55	from	abundance	2098:2106	arg1	microenvironment					2133:2148	the tumor microenvironment	2123:2148	the tumor microenvironment	2123:2148	We found that dozens of miRNAs are differentially regulated in CRC tumors and adjacent normal colon and that these miRNAs are correlated with the abundance of microbes in the tumor microenvironment.
29795787	12	56	theme	noncoding	1752:1760	arg1	molecules					1766:1774	small noncoding RNA molecules	1746:1774	small noncoding RNA molecules that can regulate gene expression and have important roles in cancer development	1746:1855	MicroRNAs (miRNAs) are small noncoding RNA molecules that can regulate gene expression and have important roles in cancer development.
29795787	12	56	theme	noncoding	1752:1760	arg1	MicroRNAs					1723:1731	MicroRNAs	1723:1731	MicroRNAs (miRNAs)	1723:1740	MicroRNAs (miRNAs) are small noncoding RNA molecules that can regulate gene expression and have important roles in cancer development.
29795787	8	57	theme	host-microbiome	1214:1228	arg1	interactions					1230:1241	host-microbiome interactions	1214:1241	host-microbiome interactions	1214:1241	Additionally, we found that miRNAs that correlated with CRC-associated bacteria are predicted to regulate targets that are relevant for host-microbiome interactions and highlight a possible role for miRNA-driven glycan production in the recruitment of pathogenic microbial taxa.
29795787	11	58	theme	gene	1692:1695	arg1	expression					1697:1706	gene expression	1692:1706	gene expression in host cells	1692:1720	One potential mechanism by which the microbiota can influence host physiology is through affecting gene expression in host cells.
29795787	7	59	theme	predicted	1046:1054	arg1	categories					1066:1075	distinct predicted metabolic categories	1037:1075	distinct predicted metabolic categories	1037:1075	Bacteria correlated with DE miRNAs were enriched with distinct predicted metabolic categories.
29795787	9	60	theme	CRC	1472:1474	arg1	tissues					1476:1482	human CRC tissues	1466:1482	human CRC tissues	1466:1482	Our work characterized a global relationship between microbial community composition and miRNA expression in human CRC tissues.
29795787	4	61	theme	normal	642:647	arg1	tissues					649:655	human CRC tumor and normal tissues	622:655	tissues	649:655	Here, we investigated the association between miRNA expression and microbiome composition in human CRC tumor and normal tissues.
29795787	6	62	theme	taxa	920:923	arg1	abundances					888:897	the relative abundances	875:897	the relative abundances of several bacterial taxa, including Firmicutes, Bacteroidetes, and Proteobacteria	875:980	These DE miRNAs were correlated with the relative abundances of several bacterial taxa, including Firmicutes, Bacteroidetes, and Proteobacteria.
29795787	4	63	from	composition	607:617	arg1	tumor					632:636	human CRC tumor and normal tissues	622:655	tumor	632:636	Here, we investigated the association between miRNA expression and microbiome composition in human CRC tumor and normal tissues.
29795787	4	63	from	composition	607:617	arg1	tissues					649:655	human CRC tumor and normal tissues	622:655	tissues	649:655	Here, we investigated the association between miRNA expression and microbiome composition in human CRC tumor and normal tissues.
29795787	6	64	theme	several	902:908	arg1	Bacteroidetes					948:960	Bacteroidetes	948:960	Bacteroidetes	948:960	These DE miRNAs were correlated with the relative abundances of several bacterial taxa, including Firmicutes, Bacteroidetes, and Proteobacteria.
29795787	6	64	theme	several	902:908	arg1	taxa					920:923	several bacterial taxa	902:923	several bacterial taxa	902:923	These DE miRNAs were correlated with the relative abundances of several bacterial taxa, including Firmicutes, Bacteroidetes, and Proteobacteria.
29795787	6	64	theme	several	902:908	arg1	Firmicutes					936:945	Firmicutes	936:945	Firmicutes	936:945	These DE miRNAs were correlated with the relative abundances of several bacterial taxa, including Firmicutes, Bacteroidetes, and Proteobacteria.
29795787	6	64	theme	several	902:908	arg1	Proteobacteria					967:980	Proteobacteria	967:980	Proteobacteria	967:980	These DE miRNAs were correlated with the relative abundances of several bacterial taxa, including Firmicutes, Bacteroidetes, and Proteobacteria.
29795787	4	65	theme	human	622:626	arg1	tumor					632:636	human CRC tumor and normal tissues	622:655	tumor	632:636	Here, we investigated the association between miRNA expression and microbiome composition in human CRC tumor and normal tissues.
29795787	7	66	theme	DE	1008:1009	arg1	miRNAs					1011:1016	DE miRNAs	1008:1016	DE miRNAs	1008:1016	Bacteria correlated with DE miRNAs were enriched with distinct predicted metabolic categories.
29795787	10	67	theme	colorectal	1545:1554	arg1	CRC					1564:1566	CRC	1564:1566	CRC	1564:1566	IMPORTANCE Recent studies have found an association between colorectal cancer (CRC) and the gut microbiota.
29795787	10	67	theme	colorectal	1545:1554	arg1	cancer					1556:1561	colorectal cancer	1545:1561	colorectal cancer (CRC)	1545:1567	IMPORTANCE Recent studies have found an association between colorectal cancer (CRC) and the gut microbiota.
29795787	1	68	theme	microbiome	106:115	arg1	composition					117:127	gut microbiome composition	102:127	gut microbiome composition	102:127	Although variation in gut microbiome composition has been linked with colorectal cancer (CRC), the factors that mediate the interactions between CRC tumors and the microbiome are poorly understood.
29795787	8	69	theme	miRNA-driven	1277:1288	arg1	production					1297:1306	miRNA-driven glycan production	1277:1306	miRNA-driven glycan production	1277:1306	Additionally, we found that miRNAs that correlated with CRC-associated bacteria are predicted to regulate targets that are relevant for host-microbiome interactions and highlight a possible role for miRNA-driven glycan production in the recruitment of pathogenic microbial taxa.
29795787	5	70	from	tumors	734:739	arg1	tissue					718:723	tissue	718:723	tissue from CRC tumors and normal tissue	718:757	We identified 76 miRNAs as differentially expressed (DE) in tissue from CRC tumors and normal tissue, including the known oncogenic miRNAs miR-182, miR-503, and mir-17~92 cluster.
29795787	17	71	theme	further	2599:2605	arg1	validation					2620:2629	further experimental validation	2599:2629	further experimental validation	2599:2629	This work provides a first systems-level map of the association between microbes and host miRNAs in the context of CRC and provides targets for further experimental validation and potential interventions.
29795787	0	72	theme	Colorectal	61:70	arg1	Cancer					72:77	Colorectal Cancer	61:77	Colorectal Cancer	61:77	Interaction between Host MicroRNAs and the Gut Microbiota in Colorectal Cancer.
29795787	8	73	from	role	1268:1271	arg1	recruitment					1315:1325	the recruitment	1311:1325	the recruitment of pathogenic microbial taxa	1311:1354	Additionally, we found that miRNAs that correlated with CRC-associated bacteria are predicted to regulate targets that are relevant for host-microbiome interactions and highlight a possible role for miRNA-driven glycan production in the recruitment of pathogenic microbial taxa.
29795787	5	74	dep	expressed	700:708	arg1	miRNAs					790:795	the known oncogenic miRNAs	770:795	the known oncogenic miRNAs miR-182, miR-503, and mir-17~92 cluster	770:835	We identified 76 miRNAs as differentially expressed (DE) in tissue from CRC tumors and normal tissue, including the known oncogenic miRNAs miR-182, miR-503, and mir-17~92 cluster.
29795787	5	74	dep	expressed	700:708	arg1	DE					711:712	DE	711:712	DE	711:712	We identified 76 miRNAs as differentially expressed (DE) in tissue from CRC tumors and normal tissue, including the known oncogenic miRNAs miR-182, miR-503, and mir-17~92 cluster.
29795787	5	74	dep	expressed	700:708	arg1	miRNAs					675:680	76 miRNAs	672:680	76 miRNAs	672:680	We identified 76 miRNAs as differentially expressed (DE) in tissue from CRC tumors and normal tissue, including the known oncogenic miRNAs miR-182, miR-503, and mir-17~92 cluster.
29795787	0	75	from	MicroRNAs	25:33	arg1	Cancer					72:77	Colorectal Cancer	61:77	Colorectal Cancer	61:77	Interaction between Host MicroRNAs and the Gut Microbiota in Colorectal Cancer.
29795787	4	76	from	expression	581:590	arg1	tumor					632:636	human CRC tumor and normal tissues	622:655	tumor	632:636	Here, we investigated the association between miRNA expression and microbiome composition in human CRC tumor and normal tissues.
29795787	4	76	from	expression	581:590	arg1	tissues					649:655	human CRC tumor and normal tissues	622:655	tissues	649:655	Here, we investigated the association between miRNA expression and microbiome composition in human CRC tumor and normal tissues.
29795787	1	77	theme	colorectal	150:159	arg1	CRC					169:171	CRC	169:171	CRC	169:171	Although variation in gut microbiome composition has been linked with colorectal cancer (CRC), the factors that mediate the interactions between CRC tumors and the microbiome are poorly understood.
29795787	1	77	theme	colorectal	150:159	arg1	cancer					161:166	colorectal cancer	150:166	colorectal cancer (CRC)	150:172	Although variation in gut microbiome composition has been linked with colorectal cancer (CRC), the factors that mediate the interactions between CRC tumors and the microbiome are poorly understood.
29795787	13	78	from	microbiota	1905:1914	arg1	CRC					1947:1949	CRC	1947:1949	CRC	1947:1949	Here, we investigated the link between the gut microbiota and the expression of miRNA in CRC.
29795787	0	79	from	Microbiota	47:56	arg1	Cancer					72:77	Colorectal Cancer	61:77	Colorectal Cancer	61:77	Interaction between Host MicroRNAs and the Gut Microbiota in Colorectal Cancer.
29795787	8	80	theme	microbial	1341:1349	arg1	taxa					1351:1354	pathogenic microbial taxa	1330:1354	pathogenic microbial taxa	1330:1354	Additionally, we found that miRNAs that correlated with CRC-associated bacteria are predicted to regulate targets that are relevant for host-microbiome interactions and highlight a possible role for miRNA-driven glycan production in the recruitment of pathogenic microbial taxa.
29795787	14	81	theme	CRC	2015:2017	arg1	tumors					2019:2024	CRC tumors	2015:2024	CRC tumors	2015:2024	We found that dozens of miRNAs are differentially regulated in CRC tumors and adjacent normal colon and that these miRNAs are correlated with the abundance of microbes in the tumor microenvironment.
29795787	10	82	theme	Recent	1496:1501	arg1	studies					1503:1509	IMPORTANCE Recent studies	1485:1509	IMPORTANCE Recent studies	1485:1509	IMPORTANCE Recent studies have found an association between colorectal cancer (CRC) and the gut microbiota.
29795787	2	83	theme	patient	359:365	arg1	outcomes					376:383	patient survival outcomes	359:383	patient survival outcomes	359:383	MicroRNAs (miRNAs) are known to regulate CRC progression and are associated with patient survival outcomes.
29795787	3	84	theme	recent	399:404	arg1	studies					406:412	recent studies	399:412	recent studies	399:412	In addition, recent studies suggested that host miRNAs can also regulate bacterial growth and influence the composition of the gut microbiome.
29795787	5	85	theme	known	774:778	arg1	miRNAs					790:795	the known oncogenic miRNAs	770:795	the known oncogenic miRNAs miR-182, miR-503, and mir-17~92 cluster	770:835	We identified 76 miRNAs as differentially expressed (DE) in tissue from CRC tumors and normal tissue, including the known oncogenic miRNAs miR-182, miR-503, and mir-17~92 cluster.
29795787	15	86	theme	related	2277:2283	arg1	genes					2271:2275	genes	2271:2275	genes related to interactions with microbes	2271:2313	Moreover, we found that microbes that have been previously associated with CRC are correlated with miRNAs that regulate genes related to interactions with microbes.
29795787	9	87	from	composition	1430:1440	arg1	tissues					1476:1482	human CRC tissues	1466:1482	human CRC tissues	1466:1482	Our work characterized a global relationship between microbial community composition and miRNA expression in human CRC tissues.
29795787	11	88	theme	host	1711:1714	arg1	cells					1716:1720	host cells	1711:1720	host cells	1711:1720	One potential mechanism by which the microbiota can influence host physiology is through affecting gene expression in host cells.
29795787	3	89	theme	host	429:432	arg1	miRNAs					434:439	host miRNAs	429:439	host miRNAs	429:439	In addition, recent studies suggested that host miRNAs can also regulate bacterial growth and influence the composition of the gut microbiome.
29795787	11	90	theme	potential	1597:1605	arg1	mechanism					1607:1615	One potential mechanism	1593:1615	One potential mechanism by which the microbiota can influence host physiology	1593:1669	One potential mechanism by which the microbiota can influence host physiology is through affecting gene expression in host cells.
29795787	9	91	theme	global	1382:1387	arg1	relationship					1389:1400	a global relationship	1380:1400	a global relationship between microbial community composition and miRNA expression in human CRC tissues	1380:1482	Our work characterized a global relationship between microbial community composition and miRNA expression in human CRC tissues.
29795787	12	92	theme	cancer	1838:1843	arg1	development					1845:1855	cancer development	1838:1855	cancer development	1838:1855	MicroRNAs (miRNAs) are small noncoding RNA molecules that can regulate gene expression and have important roles in cancer development.
29795787	17	93	theme	systems-level	2482:2494	arg1	map					2496:2498	a first systems-level map	2474:2498	a first systems-level map of the association between microbes and host miRNAs	2474:2550	This work provides a first systems-level map of the association between microbes and host miRNAs in the context of CRC and provides targets for further experimental validation and potential interventions.
29795787	14	94	theme	adjacent	2030:2037	arg1	colon					2046:2050	adjacent normal colon	2030:2050	adjacent normal colon	2030:2050	We found that dozens of miRNAs are differentially regulated in CRC tumors and adjacent normal colon and that these miRNAs are correlated with the abundance of microbes in the tumor microenvironment.
29795787	16	95	theme	pathogenic	2415:2424	arg1	taxa					2436:2439	pathogenic microbial taxa	2415:2439	pathogenic microbial taxa	2415:2439	Notably, these miRNAs likely regulate glycan production, which is important for the recruitment of pathogenic microbial taxa to the tumor.
29795787	4	96	theme	microbiome	596:605	arg1	composition					607:617	microbiome composition	596:617	microbiome composition	596:617	Here, we investigated the association between miRNA expression and microbiome composition in human CRC tumor and normal tissues.
29795787	1	97	theme	CRC	225:227	arg1	tumors					229:234	CRC tumors	225:234	CRC tumors	225:234	Although variation in gut microbiome composition has been linked with colorectal cancer (CRC), the factors that mediate the interactions between CRC tumors and the microbiome are poorly understood.
29795787	16	98	theme	taxa	2436:2439	arg1	recruitment					2400:2410	the recruitment	2396:2410	the recruitment of pathogenic microbial taxa to the tumor	2396:2452	Notably, these miRNAs likely regulate glycan production, which is important for the recruitment of pathogenic microbial taxa to the tumor.
29795787	9	99	theme	community	1420:1428	arg1	composition					1430:1440	microbial community composition	1410:1440	microbial community composition	1410:1440	Our work characterized a global relationship between microbial community composition and miRNA expression in human CRC tissues.
30972059	3	0	theme	similar	656:662	arg1	features					647:654	many features	642:654	many features similar to IBD	642:669	During DSS induced colitis, mice also develop a severe inflammation of terminal ileum with many features similar to IBD.
30972059	9	1	theme	ileal	1618:1622	arg1	insult					1636:1641	reduced ileal and colonic insult	1610:1641	reduced ileal and colonic insult	1610:1641	Results: DSS Mice treated with TLR4 inhibitor, C34, had a significantly improved disease phenotype characterized by reduced ileal and colonic insult.
30972059	1	2	with	number	314:319	arg1	little					346:351	little	346:351	little	346:351	Background: Inflammatory bowel disease (IBD) is characterized by both acute and chronic phase inflammation of the gastro-intestinal (GI) tract that affect a large and growing number of people worldwide with little to no effective treatments.
30972059	4	3	theme	DSS-treated	817:827	arg1	mice					829:832	DSS-treated mice	817:832	DSS-treated mice	817:832	As well as inflammation within the ileum we have previously demonstrated lymphatic remodeling within the mesentery and mesenteric lymph nodes of DSS-treated mice.
30972059	12	4	theme	mesenteric	2129:2138	arg1	lymphatics					2140:2149	the mesenteric lymphatics	2125:2149	the mesenteric lymphatics	2125:2149	Conclusions: TLR4 appears to mediate several changes within the mesenteric lymphatics, more specifically it is shown to have different outcomes whether stimulation occurs through pathogen derived factors such as LPS or tissue derived DAMPs, a novel phenomenon.
30972059	10	5	theme	mesenteric	1708:1717	arg1	inflammation					1719:1730	colonic and mesenteric inflammation	1696:1730	colonic and mesenteric inflammation	1696:1730	The change correlated with significant reduction in colonic and mesenteric inflammation, resolved mesenteric lymphangiectasia, and CD103+ DC migration similar to that of healthy control.
30972059	5	6	theme	lymphatic	839:847	arg1	remodeling					849:858	The lymphatic remodeling	835:858	The lymphatic remodeling	835:858	The lymphatic remodeling includes lymphangiogenesis, lymphatic vessel dilation and leakiness, as well as cellular infiltration into the surrounding tissue and peripheral draining lymph nodes.
30972059	10	7	from	reduction	1683:1691	arg1	migration					1785:1793	CD103+ DC migration	1775:1793	CD103+ DC migration	1775:1793	The change correlated with significant reduction in colonic and mesenteric inflammation, resolved mesenteric lymphangiectasia, and CD103+ DC migration similar to that of healthy control.
30972059	10	7	from	reduction	1683:1691	arg1	lymphangiectasia					1753:1768	resolved mesenteric lymphangiectasia	1733:1768	resolved mesenteric lymphangiectasia	1733:1768	The change correlated with significant reduction in colonic and mesenteric inflammation, resolved mesenteric lymphangiectasia, and CD103+ DC migration similar to that of healthy control.
30972059	10	7	from	reduction	1683:1691	arg1	inflammation					1719:1730	colonic and mesenteric inflammation	1696:1730	colonic and mesenteric inflammation	1696:1730	The change correlated with significant reduction in colonic and mesenteric inflammation, resolved mesenteric lymphangiectasia, and CD103+ DC migration similar to that of healthy control.
30972059	11	8	theme	CD103+	2044:2049	arg1	migration					2054:2062	CD103+ DC migration	2044:2062	CD103+ DC migration	2044:2062	PMXB treatment however did not resolve inflammation within the colon or associated mesenteric lymphatic dysfunction but did however prevent lymphadenopathy within the MLN through alteration of CCL21 gradients and CD103+ DC migration.
30972059	8	9	from	effect	1386:1391	arg1	composition					1467:1477	mesenteric lymph node cellular composition	1436:1477	mesenteric lymph node cellular composition	1436:1477	TLR activity and subsequent effect on lymphangiogenesis, lymphadenopathy, and mesenteric lymph node cellular composition were assessed.
30972059	8	9	from	effect	1386:1391	arg1	lymphadenopathy					1415:1429	lymphadenopathy	1415:1429	lymphadenopathy	1415:1429	TLR activity and subsequent effect on lymphangiogenesis, lymphadenopathy, and mesenteric lymph node cellular composition were assessed.
30972059	8	9	from	effect	1386:1391	arg1	lymphangiogenesis					1396:1412	lymphangiogenesis	1396:1412	lymphangiogenesis	1396:1412	TLR activity and subsequent effect on lymphangiogenesis, lymphadenopathy, and mesenteric lymph node cellular composition were assessed.
30972059	10	10	theme	CD103+	1775:1780	arg1	migration					1785:1793	CD103+ DC migration	1775:1793	CD103+ DC migration	1775:1793	The change correlated with significant reduction in colonic and mesenteric inflammation, resolved mesenteric lymphangiectasia, and CD103+ DC migration similar to that of healthy control.
30972059	12	11	theme	several	2102:2108	arg1	changes					2110:2116	several changes	2102:2116	several changes within the mesenteric lymphatics	2102:2149	Conclusions: TLR4 appears to mediate several changes within the mesenteric lymphatics, more specifically it is shown to have different outcomes whether stimulation occurs through pathogen derived factors such as LPS or tissue derived DAMPs, a novel phenomenon.
30972059	10	12	theme	mesenteric	1742:1751	arg1	lymphangiectasia					1753:1768	resolved mesenteric lymphangiectasia	1733:1768	resolved mesenteric lymphangiectasia	1733:1768	The change correlated with significant reduction in colonic and mesenteric inflammation, resolved mesenteric lymphangiectasia, and CD103+ DC migration similar to that of healthy control.
30972059	1	13	theme	acute	209:213	arg1	inflammation					233:244	both acute and chronic phase inflammation	204:244	both acute and chronic phase inflammation of the gastro-intestinal (GI) tract that affect a large and growing number of people worldwide with little to no effective treatments	204:378	Background: Inflammatory bowel disease (IBD) is characterized by both acute and chronic phase inflammation of the gastro-intestinal (GI) tract that affect a large and growing number of people worldwide with little to no effective treatments.
30972059	2	14	theme	systems	519:525	arg1	involvement					498:508	the currently poorly described involvement	467:508	the disease pathogenesis and also the currently poorly described involvement of other systems such as the lymphatics	433:548	This is in part due to the lack of understanding of the disease pathogenesis and also the currently poorly described involvement of other systems such as the lymphatics.
30972059	7	15	theme	lymphatic	1324:1332	arg1	function					1334:1341	lymphatic function	1324:1341	lymphatic function	1324:1341	Mice were treated with TLR4 blocker C34 or Polymyxin-B (PMXB) daily from days 3 to 7 of DSS treatment via I.P. injection, and their therapeutic effects on disease activity and lymphatic function were examined.
30972059	1	16	theme	chronic	219:225	arg1	inflammation					233:244	both acute and chronic phase inflammation	204:244	both acute and chronic phase inflammation of the gastro-intestinal (GI) tract that affect a large and growing number of people worldwide with little to no effective treatments	204:378	Background: Inflammatory bowel disease (IBD) is characterized by both acute and chronic phase inflammation of the gastro-intestinal (GI) tract that affect a large and growing number of people worldwide with little to no effective treatments.
30972059	3	17	theme	induced	562:568	arg1	colitis					570:576	DSS induced colitis	558:576	DSS induced colitis	558:576	During DSS induced colitis, mice also develop a severe inflammation of terminal ileum with many features similar to IBD.
30972059	1	18	theme	Inflammatory	151:162	arg1	disease					170:176	Inflammatory bowel disease	151:176	Inflammatory bowel disease (IBD)	151:182	Background: Inflammatory bowel disease (IBD) is characterized by both acute and chronic phase inflammation of the gastro-intestinal (GI) tract that affect a large and growing number of people worldwide with little to no effective treatments.
30972059	1	18	theme	Inflammatory	151:162	arg1	IBD					179:181	IBD	179:181	IBD	179:181	Background: Inflammatory bowel disease (IBD) is characterized by both acute and chronic phase inflammation of the gastro-intestinal (GI) tract that affect a large and growing number of people worldwide with little to no effective treatments.
30972059	12	19	link	derived	2253:2259	arg1	LPS					2277:2279	LPS	2277:2279	LPS	2277:2279	Conclusions: TLR4 appears to mediate several changes within the mesenteric lymphatics, more specifically it is shown to have different outcomes whether stimulation occurs through pathogen derived factors such as LPS or tissue derived DAMPs, a novel phenomenon.
30972059	12	19	link	derived	2253:2259	arg1	tissue					2284:2289	tissue	2284:2289	tissue	2284:2289	Conclusions: TLR4 appears to mediate several changes within the mesenteric lymphatics, more specifically it is shown to have different outcomes whether stimulation occurs through pathogen derived factors such as LPS or tissue derived DAMPs, a novel phenomenon.
30972059	12	19	link	derived	2253:2259	arg1	factors					2261:2267	pathogen derived factors	2244:2267	pathogen derived factors such as LPS or tissue derived DAMPs, a novel phenomenon	2244:2323	Conclusions: TLR4 appears to mediate several changes within the mesenteric lymphatics, more specifically it is shown to have different outcomes whether stimulation occurs through pathogen derived factors such as LPS or tissue derived DAMPs, a novel phenomenon.
30972059	8	20	theme	mesenteric	1436:1445	arg1	node					1453:1456	mesenteric lymph node	1436:1456	mesenteric lymph node cellular composition	1436:1477	TLR activity and subsequent effect on lymphangiogenesis, lymphadenopathy, and mesenteric lymph node cellular composition were assessed.
30972059	7	21	theme	therapeutic	1280:1290	arg1	effects					1292:1298	their therapeutic effects	1274:1298	their therapeutic effects on disease activity and lymphatic function	1274:1341	Mice were treated with TLR4 blocker C34 or Polymyxin-B (PMXB) daily from days 3 to 7 of DSS treatment via I.P. injection, and their therapeutic effects on disease activity and lymphatic function were examined.
30972059	12	22	dep	derived	2253:2259	arg1	pathogen					2244:2251	pathogen	2244:2251	pathogen	2244:2251	Conclusions: TLR4 appears to mediate several changes within the mesenteric lymphatics, more specifically it is shown to have different outcomes whether stimulation occurs through pathogen derived factors such as LPS or tissue derived DAMPs, a novel phenomenon.
30972059	3	23	theme	DSS	558:560	arg1	colitis					570:576	DSS induced colitis	558:576	DSS induced colitis	558:576	During DSS induced colitis, mice also develop a severe inflammation of terminal ileum with many features similar to IBD.
30972059	8	24	theme	node	1453:1456	arg1	composition					1467:1477	mesenteric lymph node cellular composition	1436:1477	mesenteric lymph node cellular composition	1436:1477	TLR activity and subsequent effect on lymphangiogenesis, lymphadenopathy, and mesenteric lymph node cellular composition were assessed.
30972059	1	25	theme	worldwide	331:339	arg1	number					314:319	a large and growing number	294:319	a large and growing number of people worldwide with little to no effective treatments	294:378	Background: Inflammatory bowel disease (IBD) is characterized by both acute and chronic phase inflammation of the gastro-intestinal (GI) tract that affect a large and growing number of people worldwide with little to no effective treatments.
30972059	5	26	theme	vessel	898:903	arg1	dilation					905:912	lymphatic vessel dilation	888:912	lymphatic vessel dilation	888:912	The lymphatic remodeling includes lymphangiogenesis, lymphatic vessel dilation and leakiness, as well as cellular infiltration into the surrounding tissue and peripheral draining lymph nodes.
30972059	5	27	theme	cellular	940:947	arg1	infiltration					949:960	cellular infiltration	940:960	cellular infiltration into the surrounding tissue and peripheral draining lymph nodes	940:1024	The lymphatic remodeling includes lymphangiogenesis, lymphatic vessel dilation and leakiness, as well as cellular infiltration into the surrounding tissue and peripheral draining lymph nodes.
30972059	2	28	theme	described	488:496	arg1	involvement					498:508	the currently poorly described involvement	467:508	the disease pathogenesis and also the currently poorly described involvement of other systems such as the lymphatics	433:548	This is in part due to the lack of understanding of the disease pathogenesis and also the currently poorly described involvement of other systems such as the lymphatics.
30972059	11	29	theme	lymphatic	1925:1933	arg1	dysfunction					1935:1945	associated mesenteric lymphatic dysfunction	1903:1945	associated mesenteric lymphatic dysfunction	1903:1945	PMXB treatment however did not resolve inflammation within the colon or associated mesenteric lymphatic dysfunction but did however prevent lymphadenopathy within the MLN through alteration of CCL21 gradients and CD103+ DC migration.
30972059	4	30	theme	lymphatic	745:753	arg1	remodeling					755:764	lymphatic remodeling	745:764	lymphatic remodeling within the mesentery and mesenteric lymph nodes of DSS-treated mice	745:832	As well as inflammation within the ileum we have previously demonstrated lymphatic remodeling within the mesentery and mesenteric lymph nodes of DSS-treated mice.
30972059	0	31	theme	Discriminative	116:129	arg1	Manner					131:136	a TLR4-PAMP/DAMP Discriminative Manner	99:136	a TLR4-PAMP/DAMP Discriminative Manner	99:136	Mesenteric Lymphatic Alterations Observed During DSS Induced Intestinal Inflammation Are Driven in a TLR4-PAMP/DAMP Discriminative Manner.
30972059	12	32	contain	have	2185:2188	arg1	it					2170:2171	it	2170:2171	it	2170:2171	Conclusions: TLR4 appears to mediate several changes within the mesenteric lymphatics, more specifically it is shown to have different outcomes whether stimulation occurs through pathogen derived factors such as LPS or tissue derived DAMPs, a novel phenomenon.
30972059	12	32	contain	have	2185:2188	arg2	outcomes					2200:2207	different outcomes	2190:2207	different outcomes	2190:2207	Conclusions: TLR4 appears to mediate several changes within the mesenteric lymphatics, more specifically it is shown to have different outcomes whether stimulation occurs through pathogen derived factors such as LPS or tissue derived DAMPs, a novel phenomenon.
30972059	5	33	theme	surrounding	971:981	arg1	tissue					983:988	the surrounding tissue	967:988	the surrounding tissue	967:988	The lymphatic remodeling includes lymphangiogenesis, lymphatic vessel dilation and leakiness, as well as cellular infiltration into the surrounding tissue and peripheral draining lymph nodes.
30972059	6	34	theme	C57BL/6	1075:1081	arg1	mice					1083:1086	C57BL/6 mice	1075:1086	C57BL/6 mice	1075:1086	Methods: Intestinal inflammation was induced in C57BL/6 mice by administration of 2.5% DSS in drinking water for 7 days.
30972059	2	35	theme	understanding	416:428	arg1	lack					408:411	the lack	404:411	the lack of understanding of the disease pathogenesis and also the currently poorly described involvement of other systems such as the lymphatics	404:548	This is in part due to the lack of understanding of the disease pathogenesis and also the currently poorly described involvement of other systems such as the lymphatics.
30972059	0	36	theme	Mesenteric	0:9	arg1	Alterations					21:31	Mesenteric Lymphatic Alterations	0:31	Mesenteric Lymphatic Alterations Observed During DSS Induced Intestinal Inflammation	0:83	Mesenteric Lymphatic Alterations Observed During DSS Induced Intestinal Inflammation Are Driven in a TLR4-PAMP/DAMP Discriminative Manner.
30972059	1	37	theme	tract	276:280	arg1	inflammation					233:244	both acute and chronic phase inflammation	204:244	both acute and chronic phase inflammation of the gastro-intestinal (GI) tract that affect a large and growing number of people worldwide with little to no effective treatments	204:378	Background: Inflammatory bowel disease (IBD) is characterized by both acute and chronic phase inflammation of the gastro-intestinal (GI) tract that affect a large and growing number of people worldwide with little to no effective treatments.
30972059	9	38	theme	colonic	1628:1634	arg1	insult					1636:1641	reduced ileal and colonic insult	1610:1641	reduced ileal and colonic insult	1610:1641	Results: DSS Mice treated with TLR4 inhibitor, C34, had a significantly improved disease phenotype characterized by reduced ileal and colonic insult.
30972059	6	39	theme	Intestinal	1036:1045	arg1	inflammation					1047:1058	Intestinal inflammation	1036:1058	Intestinal inflammation	1036:1058	Methods: Intestinal inflammation was induced in C57BL/6 mice by administration of 2.5% DSS in drinking water for 7 days.
30972059	11	40	theme	mesenteric	1914:1923	arg1	dysfunction					1935:1945	associated mesenteric lymphatic dysfunction	1903:1945	associated mesenteric lymphatic dysfunction	1903:1945	PMXB treatment however did not resolve inflammation within the colon or associated mesenteric lymphatic dysfunction but did however prevent lymphadenopathy within the MLN through alteration of CCL21 gradients and CD103+ DC migration.
30972059	7	41	dep	days	1221:1224	arg1	to					1228:1229	to	1228:1229	to	1228:1229	Mice were treated with TLR4 blocker C34 or Polymyxin-B (PMXB) daily from days 3 to 7 of DSS treatment via I.P. injection, and their therapeutic effects on disease activity and lymphatic function were examined.
30972059	2	42	theme	pathogenesis	445:456	arg1	understanding					416:428	understanding	416:428	understanding of the disease pathogenesis and also the currently poorly described involvement of other systems such as the lymphatics	416:548	This is in part due to the lack of understanding of the disease pathogenesis and also the currently poorly described involvement of other systems such as the lymphatics.
30972059	3	43	theme	terminal	622:629	arg1	ileum					631:635	terminal ileum	622:635	terminal ileum	622:635	During DSS induced colitis, mice also develop a severe inflammation of terminal ileum with many features similar to IBD.
30972059	4	44	theme	mesenteric	791:800	arg1	nodes					808:812	mesenteric lymph nodes	791:812	mesenteric lymph nodes	791:812	As well as inflammation within the ileum we have previously demonstrated lymphatic remodeling within the mesentery and mesenteric lymph nodes of DSS-treated mice.
30972059	1	45	theme	large	296:300	arg1	number					314:319	a large and growing number	294:319	a large and growing number of people worldwide with little to no effective treatments	294:378	Background: Inflammatory bowel disease (IBD) is characterized by both acute and chronic phase inflammation of the gastro-intestinal (GI) tract that affect a large and growing number of people worldwide with little to no effective treatments.
30972059	7	46	theme	treatment	1240:1248	arg1	days					1221:1224	days 3 to 7	1221:1231	days 3 to 7 of DSS treatment	1221:1248	Mice were treated with TLR4 blocker C34 or Polymyxin-B (PMXB) daily from days 3 to 7 of DSS treatment via I.P. injection, and their therapeutic effects on disease activity and lymphatic function were examined.
30972059	8	47	theme	TLR	1358:1360	arg1	activity					1362:1369	TLR activity	1358:1369	TLR activity	1358:1369	TLR activity and subsequent effect on lymphangiogenesis, lymphadenopathy, and mesenteric lymph node cellular composition were assessed.
30972059	10	48	theme	significant	1671:1681	arg1	reduction					1683:1691	significant reduction	1671:1691	significant reduction in colonic and mesenteric inflammation, resolved mesenteric lymphangiectasia, and CD103+ DC migration similar to that of healthy control	1671:1828	The change correlated with significant reduction in colonic and mesenteric inflammation, resolved mesenteric lymphangiectasia, and CD103+ DC migration similar to that of healthy control.
30972059	0	49	theme	Induced	53:59	arg1	Inflammation					72:83	DSS Induced Intestinal Inflammation	49:83	DSS Induced Intestinal Inflammation	49:83	Mesenteric Lymphatic Alterations Observed During DSS Induced Intestinal Inflammation Are Driven in a TLR4-PAMP/DAMP Discriminative Manner.
30972059	1	50	theme	growing	306:312	arg1	number					314:319	a large and growing number	294:319	a large and growing number of people worldwide with little to no effective treatments	294:378	Background: Inflammatory bowel disease (IBD) is characterized by both acute and chronic phase inflammation of the gastro-intestinal (GI) tract that affect a large and growing number of people worldwide with little to no effective treatments.
30972059	1	51	theme	phase	227:231	arg1	inflammation					233:244	both acute and chronic phase inflammation	204:244	both acute and chronic phase inflammation of the gastro-intestinal (GI) tract that affect a large and growing number of people worldwide with little to no effective treatments	204:378	Background: Inflammatory bowel disease (IBD) is characterized by both acute and chronic phase inflammation of the gastro-intestinal (GI) tract that affect a large and growing number of people worldwide with little to no effective treatments.
30972059	9	52	theme	disease	1575:1581	arg1	phenotype					1583:1591	a significantly improved disease phenotype	1550:1591	a significantly improved disease phenotype characterized by reduced ileal and colonic insult	1550:1641	Results: DSS Mice treated with TLR4 inhibitor, C34, had a significantly improved disease phenotype characterized by reduced ileal and colonic insult.
30972059	6	53	theme	%	1112:1112	arg1	DSS					1114:1116	2.5% DSS	1109:1116	2.5% DSS	1109:1116	Methods: Intestinal inflammation was induced in C57BL/6 mice by administration of 2.5% DSS in drinking water for 7 days.
30972059	11	54	theme	associated	1903:1912	arg1	dysfunction					1935:1945	associated mesenteric lymphatic dysfunction	1903:1945	associated mesenteric lymphatic dysfunction	1903:1945	PMXB treatment however did not resolve inflammation within the colon or associated mesenteric lymphatic dysfunction but did however prevent lymphadenopathy within the MLN through alteration of CCL21 gradients and CD103+ DC migration.
30972059	5	55	theme	draining	1005:1012	arg1	nodes					1020:1024	peripheral draining lymph nodes	994:1024	peripheral draining lymph nodes	994:1024	The lymphatic remodeling includes lymphangiogenesis, lymphatic vessel dilation and leakiness, as well as cellular infiltration into the surrounding tissue and peripheral draining lymph nodes.
30972059	1	56	theme	gastro-intestinal	253:269	arg1	tract					276:280	the gastro-intestinal (GI) tract	249:280	the gastro-intestinal (GI) tract	249:280	Background: Inflammatory bowel disease (IBD) is characterized by both acute and chronic phase inflammation of the gastro-intestinal (GI) tract that affect a large and growing number of people worldwide with little to no effective treatments.
30972059	9	57	theme	reduced	1610:1616	arg1	insult					1636:1641	reduced ileal and colonic insult	1610:1641	reduced ileal and colonic insult	1610:1641	Results: DSS Mice treated with TLR4 inhibitor, C34, had a significantly improved disease phenotype characterized by reduced ileal and colonic insult.
30972059	12	58	theme	different	2190:2198	arg1	outcomes					2200:2207	different outcomes	2190:2207	different outcomes	2190:2207	Conclusions: TLR4 appears to mediate several changes within the mesenteric lymphatics, more specifically it is shown to have different outcomes whether stimulation occurs through pathogen derived factors such as LPS or tissue derived DAMPs, a novel phenomenon.
30972059	10	59	theme	resolved	1733:1740	arg1	lymphangiectasia					1753:1768	resolved mesenteric lymphangiectasia	1733:1768	resolved mesenteric lymphangiectasia	1733:1768	The change correlated with significant reduction in colonic and mesenteric inflammation, resolved mesenteric lymphangiectasia, and CD103+ DC migration similar to that of healthy control.
30972059	1	60	theme	GI	272:273	arg1	tract					276:280	the gastro-intestinal (GI) tract	249:280	the gastro-intestinal (GI) tract	249:280	Background: Inflammatory bowel disease (IBD) is characterized by both acute and chronic phase inflammation of the gastro-intestinal (GI) tract that affect a large and growing number of people worldwide with little to no effective treatments.
30972059	11	61	theme	PMXB	1831:1834	arg1	treatment					1836:1844	PMXB treatment	1831:1844	PMXB treatment	1831:1844	PMXB treatment however did not resolve inflammation within the colon or associated mesenteric lymphatic dysfunction but did however prevent lymphadenopathy within the MLN through alteration of CCL21 gradients and CD103+ DC migration.
30972059	4	62	dep	mesentery	777:785	arg1	the					773:775	the	773:775	the	773:775	As well as inflammation within the ileum we have previously demonstrated lymphatic remodeling within the mesentery and mesenteric lymph nodes of DSS-treated mice.
30972059	4	63	theme	mice	829:832	arg1	mesentery					777:785	mesentery	777:785	mesentery	777:785	As well as inflammation within the ileum we have previously demonstrated lymphatic remodeling within the mesentery and mesenteric lymph nodes of DSS-treated mice.
30972059	4	63	theme	mice	829:832	arg1	nodes					808:812	mesenteric lymph nodes	791:812	mesenteric lymph nodes	791:812	As well as inflammation within the ileum we have previously demonstrated lymphatic remodeling within the mesentery and mesenteric lymph nodes of DSS-treated mice.
30972059	9	64	contain	had	1546:1548	arg2	phenotype					1583:1591	a significantly improved disease phenotype	1550:1591	a significantly improved disease phenotype characterized by reduced ileal and colonic insult	1550:1641	Results: DSS Mice treated with TLR4 inhibitor, C34, had a significantly improved disease phenotype characterized by reduced ileal and colonic insult.
30972059	9	64	contain	had	1546:1548	arg1	Mice					1507:1510	DSS Mice	1503:1510	DSS Mice treated with TLR4 inhibitor, C34,	1503:1544	Results: DSS Mice treated with TLR4 inhibitor, C34, had a significantly improved disease phenotype characterized by reduced ileal and colonic insult.
30972059	11	65	theme	CCL21	2024:2028	arg1	gradients					2030:2038	CCL21 gradients	2024:2038	CCL21 gradients	2024:2038	PMXB treatment however did not resolve inflammation within the colon or associated mesenteric lymphatic dysfunction but did however prevent lymphadenopathy within the MLN through alteration of CCL21 gradients and CD103+ DC migration.
30972059	3	66	with	inflammation	606:617	arg1	features					647:654	many features	642:654	many features similar to IBD	642:669	During DSS induced colitis, mice also develop a severe inflammation of terminal ileum with many features similar to IBD.
30972059	10	67	theme	similar	1795:1801	arg1	reduction					1683:1691	significant reduction	1671:1691	significant reduction in colonic and mesenteric inflammation, resolved mesenteric lymphangiectasia, and CD103+ DC migration similar to that of healthy control	1671:1828	The change correlated with significant reduction in colonic and mesenteric inflammation, resolved mesenteric lymphangiectasia, and CD103+ DC migration similar to that of healthy control.
30972059	8	68	from	activity	1362:1369	arg1	composition					1467:1477	mesenteric lymph node cellular composition	1436:1477	mesenteric lymph node cellular composition	1436:1477	TLR activity and subsequent effect on lymphangiogenesis, lymphadenopathy, and mesenteric lymph node cellular composition were assessed.
30972059	8	68	from	activity	1362:1369	arg1	lymphadenopathy					1415:1429	lymphadenopathy	1415:1429	lymphadenopathy	1415:1429	TLR activity and subsequent effect on lymphangiogenesis, lymphadenopathy, and mesenteric lymph node cellular composition were assessed.
30972059	8	68	from	activity	1362:1369	arg1	lymphangiogenesis					1396:1412	lymphangiogenesis	1396:1412	lymphangiogenesis	1396:1412	TLR activity and subsequent effect on lymphangiogenesis, lymphadenopathy, and mesenteric lymph node cellular composition were assessed.
30972059	2	69	theme	other	513:517	arg1	lymphatics					539:548	the lymphatics	535:548	the lymphatics	535:548	This is in part due to the lack of understanding of the disease pathogenesis and also the currently poorly described involvement of other systems such as the lymphatics.
30972059	2	69	theme	other	513:517	arg1	systems					519:525	other systems	513:525	other systems such as the lymphatics	513:548	This is in part due to the lack of understanding of the disease pathogenesis and also the currently poorly described involvement of other systems such as the lymphatics.
30972059	7	70	theme	blocker	1176:1182	arg1	C34					1184:1186	TLR4 blocker C34	1171:1186	TLR4 blocker C34	1171:1186	Mice were treated with TLR4 blocker C34 or Polymyxin-B (PMXB) daily from days 3 to 7 of DSS treatment via I.P. injection, and their therapeutic effects on disease activity and lymphatic function were examined.
30972059	10	71	theme	DC	1782:1783	arg1	migration					1785:1793	CD103+ DC migration	1775:1793	CD103+ DC migration	1775:1793	The change correlated with significant reduction in colonic and mesenteric inflammation, resolved mesenteric lymphangiectasia, and CD103+ DC migration similar to that of healthy control.
30972059	12	72	theme	novel	2308:2312	arg1	phenomenon					2314:2323	a novel phenomenon	2306:2323	a novel phenomenon	2306:2323	Conclusions: TLR4 appears to mediate several changes within the mesenteric lymphatics, more specifically it is shown to have different outcomes whether stimulation occurs through pathogen derived factors such as LPS or tissue derived DAMPs, a novel phenomenon.
30972059	12	72	theme	novel	2308:2312	arg1	LPS					2277:2279	LPS	2277:2279	LPS	2277:2279	Conclusions: TLR4 appears to mediate several changes within the mesenteric lymphatics, more specifically it is shown to have different outcomes whether stimulation occurs through pathogen derived factors such as LPS or tissue derived DAMPs, a novel phenomenon.
30972059	10	73	theme	healthy	1814:1820	arg1	control					1822:1828	healthy control	1814:1828	healthy control	1814:1828	The change correlated with significant reduction in colonic and mesenteric inflammation, resolved mesenteric lymphangiectasia, and CD103+ DC migration similar to that of healthy control.
30972059	8	74	theme	lymph	1447:1451	arg1	node					1453:1456	mesenteric lymph node	1436:1456	mesenteric lymph node cellular composition	1436:1477	TLR activity and subsequent effect on lymphangiogenesis, lymphadenopathy, and mesenteric lymph node cellular composition were assessed.
30972059	7	75	theme	disease	1303:1309	arg1	activity					1311:1318	disease activity	1303:1318	disease activity	1303:1318	Mice were treated with TLR4 blocker C34 or Polymyxin-B (PMXB) daily from days 3 to 7 of DSS treatment via I.P. injection, and their therapeutic effects on disease activity and lymphatic function were examined.
30972059	1	76	theme	bowel	164:168	arg1	disease					170:176	Inflammatory bowel disease	151:176	Inflammatory bowel disease (IBD)	151:182	Background: Inflammatory bowel disease (IBD) is characterized by both acute and chronic phase inflammation of the gastro-intestinal (GI) tract that affect a large and growing number of people worldwide with little to no effective treatments.
30972059	1	76	theme	bowel	164:168	arg1	IBD					179:181	IBD	179:181	IBD	179:181	Background: Inflammatory bowel disease (IBD) is characterized by both acute and chronic phase inflammation of the gastro-intestinal (GI) tract that affect a large and growing number of people worldwide with little to no effective treatments.
30972059	8	77	theme	cellular	1458:1465	arg1	composition					1467:1477	mesenteric lymph node cellular composition	1436:1477	mesenteric lymph node cellular composition	1436:1477	TLR activity and subsequent effect on lymphangiogenesis, lymphadenopathy, and mesenteric lymph node cellular composition were assessed.
30972059	1	78	theme	people	324:329	arg1	worldwide					331:339	people worldwide	324:339	people worldwide	324:339	Background: Inflammatory bowel disease (IBD) is characterized by both acute and chronic phase inflammation of the gastro-intestinal (GI) tract that affect a large and growing number of people worldwide with little to no effective treatments.
30972059	5	79	theme	lymphatic	888:896	arg1	dilation					905:912	lymphatic vessel dilation	888:912	lymphatic vessel dilation	888:912	The lymphatic remodeling includes lymphangiogenesis, lymphatic vessel dilation and leakiness, as well as cellular infiltration into the surrounding tissue and peripheral draining lymph nodes.
30972059	0	80	theme	TLR4-PAMP/DAMP	101:114	arg1	Manner					131:136	a TLR4-PAMP/DAMP Discriminative Manner	99:136	a TLR4-PAMP/DAMP Discriminative Manner	99:136	Mesenteric Lymphatic Alterations Observed During DSS Induced Intestinal Inflammation Are Driven in a TLR4-PAMP/DAMP Discriminative Manner.
30972059	9	81	theme	DSS	1503:1505	arg1	Mice					1507:1510	DSS Mice	1503:1510	DSS Mice treated with TLR4 inhibitor, C34,	1503:1544	Results: DSS Mice treated with TLR4 inhibitor, C34, had a significantly improved disease phenotype characterized by reduced ileal and colonic insult.
30972059	11	82	theme	gradients	2030:2038	arg1	alteration					2010:2019	alteration	2010:2019	alteration of CCL21 gradients	2010:2038	PMXB treatment however did not resolve inflammation within the colon or associated mesenteric lymphatic dysfunction but did however prevent lymphadenopathy within the MLN through alteration of CCL21 gradients and CD103+ DC migration.
30972059	11	82	theme	gradients	2030:2038	arg1	migration					2054:2062	CD103+ DC migration	2044:2062	CD103+ DC migration	2044:2062	PMXB treatment however did not resolve inflammation within the colon or associated mesenteric lymphatic dysfunction but did however prevent lymphadenopathy within the MLN through alteration of CCL21 gradients and CD103+ DC migration.
30972059	2	83	theme	involvement	498:508	arg1	understanding					416:428	understanding	416:428	understanding of the disease pathogenesis and also the currently poorly described involvement of other systems such as the lymphatics	416:548	This is in part due to the lack of understanding of the disease pathogenesis and also the currently poorly described involvement of other systems such as the lymphatics.
30972059	7	84	from	effects	1292:1298	arg1	activity					1311:1318	disease activity	1303:1318	disease activity	1303:1318	Mice were treated with TLR4 blocker C34 or Polymyxin-B (PMXB) daily from days 3 to 7 of DSS treatment via I.P. injection, and their therapeutic effects on disease activity and lymphatic function were examined.
30972059	7	84	from	effects	1292:1298	arg1	function					1334:1341	lymphatic function	1324:1341	lymphatic function	1324:1341	Mice were treated with TLR4 blocker C34 or Polymyxin-B (PMXB) daily from days 3 to 7 of DSS treatment via I.P. injection, and their therapeutic effects on disease activity and lymphatic function were examined.
30972059	12	85	theme	derived	2253:2259	arg1	LPS					2277:2279	LPS	2277:2279	LPS	2277:2279	Conclusions: TLR4 appears to mediate several changes within the mesenteric lymphatics, more specifically it is shown to have different outcomes whether stimulation occurs through pathogen derived factors such as LPS or tissue derived DAMPs, a novel phenomenon.
30972059	12	85	theme	derived	2253:2259	arg1	tissue					2284:2289	tissue	2284:2289	tissue	2284:2289	Conclusions: TLR4 appears to mediate several changes within the mesenteric lymphatics, more specifically it is shown to have different outcomes whether stimulation occurs through pathogen derived factors such as LPS or tissue derived DAMPs, a novel phenomenon.
30972059	12	85	theme	derived	2253:2259	arg1	factors					2261:2267	pathogen derived factors	2244:2267	pathogen derived factors such as LPS or tissue derived DAMPs, a novel phenomenon	2244:2323	Conclusions: TLR4 appears to mediate several changes within the mesenteric lymphatics, more specifically it is shown to have different outcomes whether stimulation occurs through pathogen derived factors such as LPS or tissue derived DAMPs, a novel phenomenon.
30972059	0	86	theme	Lymphatic	11:19	arg1	Alterations					21:31	Mesenteric Lymphatic Alterations	0:31	Mesenteric Lymphatic Alterations Observed During DSS Induced Intestinal Inflammation	0:83	Mesenteric Lymphatic Alterations Observed During DSS Induced Intestinal Inflammation Are Driven in a TLR4-PAMP/DAMP Discriminative Manner.
30972059	1	87	theme	effective	359:367	arg1	treatments					369:378	no effective treatments	356:378	no effective treatments	356:378	Background: Inflammatory bowel disease (IBD) is characterized by both acute and chronic phase inflammation of the gastro-intestinal (GI) tract that affect a large and growing number of people worldwide with little to no effective treatments.
30972059	9	88	theme	TLR4	1525:1528	arg1	inhibitor					1530:1538	TLR4 inhibitor	1525:1538	TLR4 inhibitor	1525:1538	Results: DSS Mice treated with TLR4 inhibitor, C34, had a significantly improved disease phenotype characterized by reduced ileal and colonic insult.
30972059	9	88	theme	TLR4	1525:1528	arg1	C34					1541:1543	C34	1541:1543	C34	1541:1543	Results: DSS Mice treated with TLR4 inhibitor, C34, had a significantly improved disease phenotype characterized by reduced ileal and colonic insult.
30972059	11	89	theme	DC	2051:2052	arg1	migration					2054:2062	CD103+ DC migration	2044:2062	CD103+ DC migration	2044:2062	PMXB treatment however did not resolve inflammation within the colon or associated mesenteric lymphatic dysfunction but did however prevent lymphadenopathy within the MLN through alteration of CCL21 gradients and CD103+ DC migration.
30972059	2	90	theme	due	397:399	arg1	part					392:395	part	392:395	part due to the lack of understanding of the disease pathogenesis and also the currently poorly described involvement of other systems such as the lymphatics	392:548	This is in part due to the lack of understanding of the disease pathogenesis and also the currently poorly described involvement of other systems such as the lymphatics.
30972059	5	91	theme	lymph	1014:1018	arg1	nodes					1020:1024	peripheral draining lymph nodes	994:1024	peripheral draining lymph nodes	994:1024	The lymphatic remodeling includes lymphangiogenesis, lymphatic vessel dilation and leakiness, as well as cellular infiltration into the surrounding tissue and peripheral draining lymph nodes.
30972059	7	92	theme	DSS	1236:1238	arg1	treatment					1240:1248	DSS treatment	1236:1248	DSS treatment	1236:1248	Mice were treated with TLR4 blocker C34 or Polymyxin-B (PMXB) daily from days 3 to 7 of DSS treatment via I.P. injection, and their therapeutic effects on disease activity and lymphatic function were examined.
30972059	2	93	theme	disease	437:443	arg1	pathogenesis					445:456	the disease pathogenesis	433:456	the disease pathogenesis and also the currently poorly described involvement of other systems such as the lymphatics	433:548	This is in part due to the lack of understanding of the disease pathogenesis and also the currently poorly described involvement of other systems such as the lymphatics.
30972059	3	94	theme	severe	599:604	arg1	inflammation					606:617	a severe inflammation	597:617	a severe inflammation of terminal ileum with many features similar to IBD	597:669	During DSS induced colitis, mice also develop a severe inflammation of terminal ileum with many features similar to IBD.
30972059	7	95	theme	TLR4	1171:1174	arg1	C34					1184:1186	TLR4 blocker C34	1171:1186	TLR4 blocker C34	1171:1186	Mice were treated with TLR4 blocker C34 or Polymyxin-B (PMXB) daily from days 3 to 7 of DSS treatment via I.P. injection, and their therapeutic effects on disease activity and lymphatic function were examined.
30972059	0	96	theme	Intestinal	61:70	arg1	Inflammation					72:83	DSS Induced Intestinal Inflammation	49:83	DSS Induced Intestinal Inflammation	49:83	Mesenteric Lymphatic Alterations Observed During DSS Induced Intestinal Inflammation Are Driven in a TLR4-PAMP/DAMP Discriminative Manner.
30972059	3	97	theme	many	642:645	arg1	features					647:654	many features	642:654	many features similar to IBD	642:669	During DSS induced colitis, mice also develop a severe inflammation of terminal ileum with many features similar to IBD.
30972059	4	98	theme	lymph	802:806	arg1	nodes					808:812	mesenteric lymph nodes	791:812	mesenteric lymph nodes	791:812	As well as inflammation within the ileum we have previously demonstrated lymphatic remodeling within the mesentery and mesenteric lymph nodes of DSS-treated mice.
30972059	9	99	theme	improved	1566:1573	arg1	phenotype					1583:1591	a significantly improved disease phenotype	1550:1591	a significantly improved disease phenotype characterized by reduced ileal and colonic insult	1550:1641	Results: DSS Mice treated with TLR4 inhibitor, C34, had a significantly improved disease phenotype characterized by reduced ileal and colonic insult.
30972059	6	100	theme	DSS	1114:1116	arg1	administration					1091:1104	administration	1091:1104	administration of 2.5% DSS	1091:1116	Methods: Intestinal inflammation was induced in C57BL/6 mice by administration of 2.5% DSS in drinking water for 7 days.
30972059	3	101	theme	ileum	631:635	arg1	inflammation					606:617	a severe inflammation	597:617	a severe inflammation of terminal ileum with many features similar to IBD	597:669	During DSS induced colitis, mice also develop a severe inflammation of terminal ileum with many features similar to IBD.
30972059	5	102	theme	peripheral	994:1003	arg1	nodes					1020:1024	peripheral draining lymph nodes	994:1024	peripheral draining lymph nodes	994:1024	The lymphatic remodeling includes lymphangiogenesis, lymphatic vessel dilation and leakiness, as well as cellular infiltration into the surrounding tissue and peripheral draining lymph nodes.
30972059	6	103	theme	2.5	1109:1111	arg1	%					1112:1112	%	1112:1112	%	1112:1112	Methods: Intestinal inflammation was induced in C57BL/6 mice by administration of 2.5% DSS in drinking water for 7 days.
30972059	8	104	theme	subsequent	1375:1384	arg1	effect					1386:1391	subsequent effect	1375:1391	subsequent effect on lymphangiogenesis, lymphadenopathy, and mesenteric lymph node cellular composition	1375:1477	TLR activity and subsequent effect on lymphangiogenesis, lymphadenopathy, and mesenteric lymph node cellular composition were assessed.
30972059	10	105	theme	colonic	1696:1702	arg1	inflammation					1719:1730	colonic and mesenteric inflammation	1696:1730	colonic and mesenteric inflammation	1696:1730	The change correlated with significant reduction in colonic and mesenteric inflammation, resolved mesenteric lymphangiectasia, and CD103+ DC migration similar to that of healthy control.
30441885	9	0	theme	whole	980:984	arg1	sequences					993:1001	whole genome sequences	980:1001	whole genome sequences	980:1001	The phylogenetic tree based on whole genome sequences showed that each subspecies clearly formed a monophyletic group, while the tree based on tar genes showed that monophyletic groups were formed according to the cell wall type rather than the subspecies.
30441885	8	1	theme	previous	840:847	arg1	studies					849:855	previous studies	840:855	previous studies	840:855	The results and previous studies suggest that the tar genes and the tag genes are not inherited after subspecies speciation.
30441885	1	2	from	Variation	68:76	arg1	Genes					91:95	Cell Wall Genes	81:95	Cell Wall Genes of B. subtilis Strains	81:118	subtilis KCTC 3135T and Variation in Cell Wall Genes of B. subtilis Strains.
30441885	4	3	theme	distinguishing	414:427	arg1	difference					429:438	the distinguishing difference	410:438	the distinguishing difference between B. s. subtilis and B. s. spizizenii	410:482	The tar and tag genes were involved in synthesizing wall teichoic acids (WTAs), and these genes and their products were previously regarded as the distinguishing difference between B. s. subtilis and B. s. spizizenii.
30441885	4	3	theme	distinguishing	414:427	arg1	genes					357:361	genes	357:361	genes	357:361	The tar and tag genes were involved in synthesizing wall teichoic acids (WTAs), and these genes and their products were previously regarded as the distinguishing difference between B. s. subtilis and B. s. spizizenii.
30441885	4	3	theme	distinguishing	414:427	arg1	products					373:380	their products	367:380	their products	367:380	The tar and tag genes were involved in synthesizing wall teichoic acids (WTAs), and these genes and their products were previously regarded as the distinguishing difference between B. s. subtilis and B. s. spizizenii.
30441885	9	4	theme	cell	1163:1166	arg1	type					1173:1176	the cell wall type	1159:1176	the cell wall type rather than the subspecies	1159:1203	The phylogenetic tree based on whole genome sequences showed that each subspecies clearly formed a monophyletic group, while the tree based on tar genes showed that monophyletic groups were formed according to the cell wall type rather than the subspecies.
30441885	5	5	theme	subtilis	531:538	arg1	analysis					516:523	a comparative genomic analysis	494:523	a comparative genomic analysis of B. subtilis	494:538	However, a comparative genomic analysis of B. subtilis spp.
30441885	9	6	theme	monophyletic	1114:1125	arg1	groups					1127:1132	monophyletic groups	1114:1132	monophyletic groups	1114:1132	The phylogenetic tree based on whole genome sequences showed that each subspecies clearly formed a monophyletic group, while the tree based on tar genes showed that monophyletic groups were formed according to the cell wall type rather than the subspecies.
30441885	2	7	dep	Bacillus	137:144	arg1	subtilis					146:153	subtilis	146:153	subtilis	146:153	The type strain Bacillus subtilis subsp.
30441885	9	8	theme	wall	1168:1171	arg1	type					1173:1176	the cell wall type	1159:1176	the cell wall type rather than the subspecies	1159:1203	The phylogenetic tree based on whole genome sequences showed that each subspecies clearly formed a monophyletic group, while the tree based on tar genes showed that monophyletic groups were formed according to the cell wall type rather than the subspecies.
30441885	1	9	theme	KCTC	53:56	arg1	3135T					58:62	subtilis KCTC 3135T	44:62	subtilis KCTC 3135T	44:62	subtilis KCTC 3135T and Variation in Cell Wall Genes of B. subtilis Strains.
30441885	1	10	from	3135T	58:62	arg1	Genes					91:95	Cell Wall Genes	81:95	Cell Wall Genes of B. subtilis Strains	81:118	subtilis KCTC 3135T and Variation in Cell Wall Genes of B. subtilis Strains.
30441885	10	11	theme	tar	1239:1241	arg1	genes					1243:1247	the tar genes	1235:1247	the tar genes	1235:1247	These findings indicate that the tar genes and the presence of ribitol as a cell-wall constituent were not the distinguishing difference between the subspecies of B. subtilis and that the description of subspecies B. s. spizizenii should be updated.
30441885	10	11	theme	tar	1239:1241	arg1	presence					1257:1264	the presence	1253:1264	the presence of ribitol	1253:1275	These findings indicate that the tar genes and the presence of ribitol as a cell-wall constituent were not the distinguishing difference between the subspecies of B. subtilis and that the description of subspecies B. s. spizizenii should be updated.
30441885	10	11	theme	tar	1239:1241	arg1	difference					1332:1341	the distinguishing difference	1313:1341	the distinguishing difference between the subspecies of B. subtilis	1313:1379	These findings indicate that the tar genes and the presence of ribitol as a cell-wall constituent were not the distinguishing difference between the subspecies of B. subtilis and that the description of subspecies B. s. spizizenii should be updated.
30441885	2	12	theme	strain	130:135	arg1	subsp					155:159	The type strain Bacillus subtilis subsp	121:159	The type strain Bacillus subtilis subsp	121:159	The type strain Bacillus subtilis subsp.
30441885	10	13	theme	cell-wall	1282:1290	arg1	constituent					1292:1302	a cell-wall constituent	1280:1302	a cell-wall constituent	1280:1302	These findings indicate that the tar genes and the presence of ribitol as a cell-wall constituent were not the distinguishing difference between the subspecies of B. subtilis and that the description of subspecies B. s. spizizenii should be updated.
30441885	8	14	theme	tar	874:876	arg1	genes					878:882	the tar genes	870:882	the tar genes	870:882	The results and previous studies suggest that the tar genes and the tag genes are not inherited after subspecies speciation.
30441885	9	15	theme	phylogenetic	953:964	arg1	tree					966:969	The phylogenetic tree	949:969	The phylogenetic tree based on whole genome sequences	949:1001	The phylogenetic tree based on whole genome sequences showed that each subspecies clearly formed a monophyletic group, while the tree based on tar genes showed that monophyletic groups were formed according to the cell wall type rather than the subspecies.
30441885	7	16	theme	tar	639:641	arg1	operons					651:657	These tar and tag operons	633:657	operons	651:657	These tar and tag operons were mutually exclusive and the tar genes from B. s. spizizenii were very similar to the genes from non-Bacillus bacteria, unlike the tag genes from B. s. subtilis.
30441885	10	17	theme	spizizenii	1426:1435	arg1	description					1394:1404	the description	1390:1404	the description of subspecies B. s. spizizenii	1390:1435	These findings indicate that the tar genes and the presence of ribitol as a cell-wall constituent were not the distinguishing difference between the subspecies of B. subtilis and that the description of subspecies B. s. spizizenii should be updated.
30441885	2	18	theme	type	125:128	arg1	subsp					155:159	The type strain Bacillus subtilis subsp	121:159	The type strain Bacillus subtilis subsp	121:159	The type strain Bacillus subtilis subsp.
30441885	8	19	theme	subspecies	926:935	arg1	speciation					937:946	subspecies speciation	926:946	subspecies speciation	926:946	The results and previous studies suggest that the tar genes and the tag genes are not inherited after subspecies speciation.
30441885	6	20	theme	walls	626:630	arg1	types					612:616	various types	604:616	various types of cell walls	604:630	revealed that both B. s. subtilis and B. s. spizizenii had various types of cell walls.
30441885	3	21	theme	previous	230:237	arg1	genome					245:250	a previous draft genome	228:250	a previous draft genome	228:250	subtilis KCTC 3135T was deeply sequenced and annotated, replacing a previous draft genome in this study.
30441885	5	22	theme	genomic	508:514	arg1	analysis					516:523	a comparative genomic analysis	494:523	a comparative genomic analysis of B. subtilis	494:538	However, a comparative genomic analysis of B. subtilis spp.
30441885	4	23	theme	tar	271:273	arg1	genes					283:287	The tar and tag genes	267:287	genes	283:287	The tar and tag genes were involved in synthesizing wall teichoic acids (WTAs), and these genes and their products were previously regarded as the distinguishing difference between B. s. subtilis and B. s. spizizenii.
30441885	10	24	theme	distinguishing	1317:1330	arg1	genes					1243:1247	the tar genes	1235:1247	the tar genes	1235:1247	These findings indicate that the tar genes and the presence of ribitol as a cell-wall constituent were not the distinguishing difference between the subspecies of B. subtilis and that the description of subspecies B. s. spizizenii should be updated.
30441885	10	24	theme	distinguishing	1317:1330	arg1	presence					1257:1264	the presence	1253:1264	the presence of ribitol	1253:1275	These findings indicate that the tar genes and the presence of ribitol as a cell-wall constituent were not the distinguishing difference between the subspecies of B. subtilis and that the description of subspecies B. s. spizizenii should be updated.
30441885	10	24	theme	distinguishing	1317:1330	arg1	difference					1332:1341	the distinguishing difference	1313:1341	the distinguishing difference between the subspecies of B. subtilis	1313:1379	These findings indicate that the tar genes and the presence of ribitol as a cell-wall constituent were not the distinguishing difference between the subspecies of B. subtilis and that the description of subspecies B. s. spizizenii should be updated.
30441885	4	25	theme	teichoic	324:331	arg1	WTAs					340:343	WTAs	340:343	WTAs	340:343	The tar and tag genes were involved in synthesizing wall teichoic acids (WTAs), and these genes and their products were previously regarded as the distinguishing difference between B. s. subtilis and B. s. spizizenii.
30441885	4	25	theme	teichoic	324:331	arg1	acids					333:337	wall teichoic acids	319:337	wall teichoic acids (WTAs)	319:344	The tar and tag genes were involved in synthesizing wall teichoic acids (WTAs), and these genes and their products were previously regarded as the distinguishing difference between B. s. subtilis and B. s. spizizenii.
30441885	9	26	theme	monophyletic	1048:1059	arg1	group					1061:1065	a monophyletic group	1046:1065	a monophyletic group	1046:1065	The phylogenetic tree based on whole genome sequences showed that each subspecies clearly formed a monophyletic group, while the tree based on tar genes showed that monophyletic groups were formed according to the cell wall type rather than the subspecies.
30441885	7	27	from	subtilis	814:821	arg1	genes					797:801	the tag genes	789:801	the tag genes from B. s. subtilis	789:821	These tar and tag operons were mutually exclusive and the tar genes from B. s. spizizenii were very similar to the genes from non-Bacillus bacteria, unlike the tag genes from B. s. subtilis.
30441885	0	28	theme	Complete	0:7	arg1	Genome					9:14	Complete Genome	0:14	Complete Genome of Bacillus subtilis	0:35	Complete Genome of Bacillus subtilis subsp.
30441885	7	29	from	spizizenii	712:721	arg1	similar					733:739	similar	733:739	similar	733:739	These tar and tag operons were mutually exclusive and the tar genes from B. s. spizizenii were very similar to the genes from non-Bacillus bacteria, unlike the tag genes from B. s. subtilis.
30441885	7	29	from	spizizenii	712:721	arg1	genes					695:699	the tar genes	687:699	the tar genes from B. s. spizizenii	687:721	These tar and tag operons were mutually exclusive and the tar genes from B. s. spizizenii were very similar to the genes from non-Bacillus bacteria, unlike the tag genes from B. s. subtilis.
30441885	3	30	theme	subtilis	162:169	arg1	3135T					176:180	subtilis KCTC 3135T	162:180	subtilis KCTC 3135T	162:180	subtilis KCTC 3135T was deeply sequenced and annotated, replacing a previous draft genome in this study.
30441885	1	31	theme	Cell	81:84	arg1	Genes					91:95	Cell Wall Genes	81:95	Cell Wall Genes of B. subtilis Strains	81:118	subtilis KCTC 3135T and Variation in Cell Wall Genes of B. subtilis Strains.
30441885	8	32	theme	tag	892:894	arg1	genes					896:900	the tag genes	888:900	the tag genes	888:900	The results and previous studies suggest that the tar genes and the tag genes are not inherited after subspecies speciation.
30441885	3	33	theme	KCTC	171:174	arg1	3135T					176:180	subtilis KCTC 3135T	162:180	subtilis KCTC 3135T	162:180	subtilis KCTC 3135T was deeply sequenced and annotated, replacing a previous draft genome in this study.
30441885	1	34	theme	Wall	86:89	arg1	Genes					91:95	Cell Wall Genes	81:95	Cell Wall Genes of B. subtilis Strains	81:118	subtilis KCTC 3135T and Variation in Cell Wall Genes of B. subtilis Strains.
30441885	6	35	theme	various	604:610	arg1	types					612:616	various types	604:616	various types of cell walls	604:630	revealed that both B. s. subtilis and B. s. spizizenii had various types of cell walls.
30441885	9	36	theme	genome	986:991	arg1	sequences					993:1001	whole genome sequences	980:1001	whole genome sequences	980:1001	The phylogenetic tree based on whole genome sequences showed that each subspecies clearly formed a monophyletic group, while the tree based on tar genes showed that monophyletic groups were formed according to the cell wall type rather than the subspecies.
30441885	7	37	theme	non-Bacillus	759:770	arg1	bacteria					772:779	non-Bacillus bacteria	759:779	non-Bacillus bacteria	759:779	These tar and tag operons were mutually exclusive and the tar genes from B. s. spizizenii were very similar to the genes from non-Bacillus bacteria, unlike the tag genes from B. s. subtilis.
30441885	3	38	theme	draft	239:243	arg1	genome					245:250	a previous draft genome	228:250	a previous draft genome	228:250	subtilis KCTC 3135T was deeply sequenced and annotated, replacing a previous draft genome in this study.
30441885	0	39	theme	subtilis	28:35	arg1	Genome					9:14	Complete Genome	0:14	Complete Genome of Bacillus subtilis	0:35	Complete Genome of Bacillus subtilis subsp.
30441885	7	40	theme	tar	691:693	arg1	similar					733:739	similar	733:739	similar	733:739	These tar and tag operons were mutually exclusive and the tar genes from B. s. spizizenii were very similar to the genes from non-Bacillus bacteria, unlike the tag genes from B. s. subtilis.
30441885	7	40	theme	tar	691:693	arg1	genes					695:699	the tar genes	687:699	the tar genes from B. s. spizizenii	687:721	These tar and tag operons were mutually exclusive and the tar genes from B. s. spizizenii were very similar to the genes from non-Bacillus bacteria, unlike the tag genes from B. s. subtilis.
30441885	7	41	theme	tag	647:649	arg1	operons					651:657	These tar and tag operons	633:657	operons	651:657	These tar and tag operons were mutually exclusive and the tar genes from B. s. spizizenii were very similar to the genes from non-Bacillus bacteria, unlike the tag genes from B. s. subtilis.
30441885	2	42	theme	Bacillus	137:144	arg1	subsp					155:159	The type strain Bacillus subtilis subsp	121:159	The type strain Bacillus subtilis subsp	121:159	The type strain Bacillus subtilis subsp.
30441885	6	43	contain	had	600:602	arg1	spizizenii					589:598	B. s. spizizenii	583:598	B. s. spizizenii	583:598	revealed that both B. s. subtilis and B. s. spizizenii had various types of cell walls.
30441885	6	43	contain	had	600:602	arg2	types					612:616	various types	604:616	various types of cell walls	604:630	revealed that both B. s. subtilis and B. s. spizizenii had various types of cell walls.
30441885	6	43	contain	had	600:602	arg1	subtilis					570:577	B. s. subtilis	564:577	B. s. subtilis	564:577	revealed that both B. s. subtilis and B. s. spizizenii had various types of cell walls.
30441885	4	44	theme	wall	319:322	arg1	WTAs					340:343	WTAs	340:343	WTAs	340:343	The tar and tag genes were involved in synthesizing wall teichoic acids (WTAs), and these genes and their products were previously regarded as the distinguishing difference between B. s. subtilis and B. s. spizizenii.
30441885	4	44	theme	wall	319:322	arg1	acids					333:337	wall teichoic acids	319:337	wall teichoic acids (WTAs)	319:344	The tar and tag genes were involved in synthesizing wall teichoic acids (WTAs), and these genes and their products were previously regarded as the distinguishing difference between B. s. subtilis and B. s. spizizenii.
30441885	1	45	theme	B.	100:101	arg1	Strains					112:118	B. subtilis Strains	100:118	B. subtilis Strains	100:118	subtilis KCTC 3135T and Variation in Cell Wall Genes of B. subtilis Strains.
30441885	9	46	theme	tar	1092:1094	arg1	genes					1096:1100	tar genes	1092:1100	tar genes	1092:1100	The phylogenetic tree based on whole genome sequences showed that each subspecies clearly formed a monophyletic group, while the tree based on tar genes showed that monophyletic groups were formed according to the cell wall type rather than the subspecies.
30441885	5	47	theme	comparative	496:506	arg1	analysis					516:523	a comparative genomic analysis	494:523	a comparative genomic analysis of B. subtilis	494:538	However, a comparative genomic analysis of B. subtilis spp.
30441885	1	48	theme	subtilis	103:110	arg1	Strains					112:118	B. subtilis Strains	100:118	B. subtilis Strains	100:118	subtilis KCTC 3135T and Variation in Cell Wall Genes of B. subtilis Strains.
30441885	10	49	theme	subtilis	1372:1379	arg1	subspecies					1355:1364	the subspecies	1351:1364	the subspecies of B. subtilis	1351:1379	These findings indicate that the tar genes and the presence of ribitol as a cell-wall constituent were not the distinguishing difference between the subspecies of B. subtilis and that the description of subspecies B. s. spizizenii should be updated.
30441885	7	50	from	bacteria	772:779	arg1	genes					748:752	the genes	744:752	the genes from non-Bacillus bacteria	744:779	These tar and tag operons were mutually exclusive and the tar genes from B. s. spizizenii were very similar to the genes from non-Bacillus bacteria, unlike the tag genes from B. s. subtilis.
30441885	4	51	theme	tag	279:281	arg1	genes					283:287	The tar and tag genes	267:287	genes	283:287	The tar and tag genes were involved in synthesizing wall teichoic acids (WTAs), and these genes and their products were previously regarded as the distinguishing difference between B. s. subtilis and B. s. spizizenii.
30441885	1	52	theme	Strains	112:118	arg1	Genes					91:95	Cell Wall Genes	81:95	Cell Wall Genes of B. subtilis Strains	81:118	subtilis KCTC 3135T and Variation in Cell Wall Genes of B. subtilis Strains.
30441885	7	53	theme	tag	793:795	arg1	genes					797:801	the tag genes	789:801	the tag genes from B. s. subtilis	789:821	These tar and tag operons were mutually exclusive and the tar genes from B. s. spizizenii were very similar to the genes from non-Bacillus bacteria, unlike the tag genes from B. s. subtilis.
30441885	6	54	theme	cell	621:624	arg1	walls					626:630	cell walls	621:630	cell walls	621:630	revealed that both B. s. subtilis and B. s. spizizenii had various types of cell walls.
30441885	1	55	theme	subtilis	44:51	arg1	3135T					58:62	subtilis KCTC 3135T	44:62	subtilis KCTC 3135T	44:62	subtilis KCTC 3135T and Variation in Cell Wall Genes of B. subtilis Strains.
30441885	10	56	theme	ribitol	1269:1275	arg1	genes					1243:1247	the tar genes	1235:1247	the tar genes	1235:1247	These findings indicate that the tar genes and the presence of ribitol as a cell-wall constituent were not the distinguishing difference between the subspecies of B. subtilis and that the description of subspecies B. s. spizizenii should be updated.
30441885	10	56	theme	ribitol	1269:1275	arg1	presence					1257:1264	the presence	1253:1264	the presence of ribitol	1253:1275	These findings indicate that the tar genes and the presence of ribitol as a cell-wall constituent were not the distinguishing difference between the subspecies of B. subtilis and that the description of subspecies B. s. spizizenii should be updated.
30441885	10	56	theme	ribitol	1269:1275	arg1	difference					1332:1341	the distinguishing difference	1313:1341	the distinguishing difference between the subspecies of B. subtilis	1313:1379	These findings indicate that the tar genes and the presence of ribitol as a cell-wall constituent were not the distinguishing difference between the subspecies of B. subtilis and that the description of subspecies B. s. spizizenii should be updated.
30328805	5	0	theme	digital	673:679	arg1	hybridization					689:701	digital DNA-DNA hybridization	673:701	digital DNA-DNA hybridization values	673:708	Average nucleotide identity values and digital DNA-DNA hybridization values were less than 74.2 and 18.1 %, respectively, between strain 22-7T and the type strains of closely related species.
30328805	2	1	theme	bacterial	137:145	arg1	22-7T					155:159	22-7T	155:159	22-7T	155:159	A Gram-stain-positive, rod-shaped bacterial strain, 22-7T, was isolated from ocean sediment of Laizhou Bay, China, and was characterized by using a polyphasic approach.
30328805	2	1	theme	bacterial	137:145	arg1	strain					147:152	A Gram-stain-positive, rod-shaped bacterial strain	103:152	A Gram-stain-positive, rod-shaped bacterial strain	103:152	A Gram-stain-positive, rod-shaped bacterial strain, 22-7T, was isolated from ocean sediment of Laizhou Bay, China, and was characterized by using a polyphasic approach.
30328805	10	2	theme	1H00228T=KCTC	1402:1414	arg1	33930T					1416:1421	=MCCC 1H00228T=KCTC 33930T	1396:1421	=MCCC 1H00228T=KCTC 33930T	1396:1421	The type strain is 22-7T(=MCCC 1H00228T=KCTC 33930T).
30328805	10	2	theme	1H00228T=KCTC	1402:1414	arg1	22-7T					1390:1394	22-7T	1390:1394	22-7T(=MCCC 1H00228T=KCTC 33930T)	1390:1422	The type strain is 22-7T(=MCCC 1H00228T=KCTC 33930T).
30328805	5	3	theme	DNA-DNA	681:687	arg1	hybridization					689:701	digital DNA-DNA hybridization	673:701	digital DNA-DNA hybridization values	673:708	Average nucleotide identity values and digital DNA-DNA hybridization values were less than 74.2 and 18.1 %, respectively, between strain 22-7T and the type strains of closely related species.
30328805	2	4	dep	Gram-stain-positive	105:123	arg1	rod-shaped					126:135	rod-shaped	126:135	rod-shaped	126:135	A Gram-stain-positive, rod-shaped bacterial strain, 22-7T, was isolated from ocean sediment of Laizhou Bay, China, and was characterized by using a polyphasic approach.
30328805	7	5	theme	cell	1082:1085	arg1	wall					1087:1090	the cell wall	1078:1090	the cell wall	1078:1090	The peptidoglycan type of the cell wall was A1α linked through l-lysine as the diamino acid.
30328805	8	6	theme	strain	1241:1246	arg1	22-7T					1248:1252	strain 22-7T	1241:1252	strain 22-7T	1241:1252	Combined data from phenotypic, chemotaxonomic and genotypic characterizations demonstrated that strain 22-7T represents a novel Jeotgalibacillus species, for which the name Jeotgalibacillus proteolyticus sp.
30328805	8	7	theme	Jeotgalibacillus	1273:1288	arg1	species					1290:1296	a novel Jeotgalibacillus species	1265:1296	a novel Jeotgalibacillus species	1265:1296	Combined data from phenotypic, chemotaxonomic and genotypic characterizations demonstrated that strain 22-7T represents a novel Jeotgalibacillus species, for which the name Jeotgalibacillus proteolyticus sp.
30328805	4	8	theme	98.7 	530:534	arg1	similarity					537:546	98.7 % similarity	530:546	98.7 % similarity	530:546	Phylogenetic analysis based on 16S rRNA gene sequences identified it as a member of the genus Jeotgalibacillus, most similar to Jeotgalibacillus campisalis SF-57T (98.7 % similarity), Jeotgalibacillus marinus DSM 1297T (98.2 %) and Jeotgalibacillus soli P9T (97.1 %).
30328805	3	9	theme	pH	334:335	arg1	plate					325:329	a 2216E agar plate	312:329	a 2216E agar plate of pH 7.5	312:339	Optimal growth was observed at 33 °C on a 2216E agar plate of pH 7.5 and with 2 % (w/v) NaCl.
30328805	4	10	dep	Jeotgalibacillus	494:509	arg1	campisalis					511:520	campisalis	511:520	campisalis	511:520	Phylogenetic analysis based on 16S rRNA gene sequences identified it as a member of the genus Jeotgalibacillus, most similar to Jeotgalibacillus campisalis SF-57T (98.7 % similarity), Jeotgalibacillus marinus DSM 1297T (98.2 %) and Jeotgalibacillus soli P9T (97.1 %).
30328805	0	11	theme	Jeotgalibacillus	0:15	arg1	sp					31:32	Jeotgalibacillus proteolyticus sp	0:32	Jeotgalibacillus proteolyticus sp.	0:33	Jeotgalibacillus proteolyticus sp.
30328805	4	12	dep	genus	454:458	arg1	Jeotgalibacillus					460:475	Jeotgalibacillus	460:475	Jeotgalibacillus	460:475	Phylogenetic analysis based on 16S rRNA gene sequences identified it as a member of the genus Jeotgalibacillus, most similar to Jeotgalibacillus campisalis SF-57T (98.7 % similarity), Jeotgalibacillus marinus DSM 1297T (98.2 %) and Jeotgalibacillus soli P9T (97.1 %).
30328805	4	12	dep	genus	454:458	arg1	similar					483:489	similar	483:489	similar	483:489	Phylogenetic analysis based on 16S rRNA gene sequences identified it as a member of the genus Jeotgalibacillus, most similar to Jeotgalibacillus campisalis SF-57T (98.7 % similarity), Jeotgalibacillus marinus DSM 1297T (98.2 %) and Jeotgalibacillus soli P9T (97.1 %).
30328805	4	12	dep	genus	454:458	arg1	Jeotgalibacillus					550:565	Jeotgalibacillus	550:565	Jeotgalibacillus	550:565	Phylogenetic analysis based on 16S rRNA gene sequences identified it as a member of the genus Jeotgalibacillus, most similar to Jeotgalibacillus campisalis SF-57T (98.7 % similarity), Jeotgalibacillus marinus DSM 1297T (98.2 %) and Jeotgalibacillus soli P9T (97.1 %).
30328805	3	13	theme	agar	320:323	arg1	plate					325:329	a 2216E agar plate	312:329	a 2216E agar plate of pH 7.5	312:339	Optimal growth was observed at 33 °C on a 2216E agar plate of pH 7.5 and with 2 % (w/v) NaCl.
30328805	4	14	theme	gene	406:409	arg1	sequences					411:419	16S rRNA gene sequences	397:419	16S rRNA gene sequences	397:419	Phylogenetic analysis based on 16S rRNA gene sequences identified it as a member of the genus Jeotgalibacillus, most similar to Jeotgalibacillus campisalis SF-57T (98.7 % similarity), Jeotgalibacillus marinus DSM 1297T (98.2 %) and Jeotgalibacillus soli P9T (97.1 %).
30328805	3	15	dep	%	352:352	arg1	w/v					355:357	w/v	355:357	w/v	355:357	Optimal growth was observed at 33 °C on a 2216E agar plate of pH 7.5 and with 2 % (w/v) NaCl.
30328805	5	16	theme	identity	653:660	arg1	values					662:667	Average nucleotide identity values	634:667	Average nucleotide identity values	634:667	Average nucleotide identity values and digital DNA-DNA hybridization values were less than 74.2 and 18.1 %, respectively, between strain 22-7T and the type strains of closely related species.
30328805	4	17	dep	similar	483:489	arg1	similarity					537:546	98.7 % similarity	530:546	98.7 % similarity	530:546	Phylogenetic analysis based on 16S rRNA gene sequences identified it as a member of the genus Jeotgalibacillus, most similar to Jeotgalibacillus campisalis SF-57T (98.7 % similarity), Jeotgalibacillus marinus DSM 1297T (98.2 %) and Jeotgalibacillus soli P9T (97.1 %).
30328805	10	18	theme	type	1375:1378	arg1	22-7T					1390:1394	22-7T	1390:1394	22-7T(=MCCC 1H00228T=KCTC 33930T)	1390:1422	The type strain is 22-7T(=MCCC 1H00228T=KCTC 33930T).
30328805	10	18	theme	type	1375:1378	arg1	strain					1380:1385	The type strain	1371:1385	The type strain	1371:1385	The type strain is 22-7T(=MCCC 1H00228T=KCTC 33930T).
30328805	1	19	theme	ocean	86:90	arg1	sediments					92:100	ocean sediments	86:100	ocean sediments	86:100	nov., a protease-producing bacterium isolated from ocean sediments.
30328805	5	20	theme	nucleotide	642:651	arg1	values					662:667	Average nucleotide identity values	634:667	Average nucleotide identity values	634:667	Average nucleotide identity values and digital DNA-DNA hybridization values were less than 74.2 and 18.1 %, respectively, between strain 22-7T and the type strains of closely related species.
30328805	0	21	theme	proteolyticus	17:29	arg1	sp					31:32	Jeotgalibacillus proteolyticus sp	0:32	Jeotgalibacillus proteolyticus sp.	0:33	Jeotgalibacillus proteolyticus sp.
30328805	8	22	from	characterizations	1205:1221	arg1	data					1154:1157	Combined data	1145:1157	Combined data from phenotypic, chemotaxonomic and genotypic characterizations	1145:1221	Combined data from phenotypic, chemotaxonomic and genotypic characterizations demonstrated that strain 22-7T represents a novel Jeotgalibacillus species, for which the name Jeotgalibacillus proteolyticus sp.
30328805	8	23	theme	novel	1267:1271	arg1	species					1290:1296	a novel Jeotgalibacillus species	1265:1296	a novel Jeotgalibacillus species	1265:1296	Combined data from phenotypic, chemotaxonomic and genotypic characterizations demonstrated that strain 22-7T represents a novel Jeotgalibacillus species, for which the name Jeotgalibacillus proteolyticus sp.
30328805	7	24	theme	peptidoglycan	1056:1068	arg1	acid					1139:1142	the diamino acid	1127:1142	the diamino acid	1127:1142	The peptidoglycan type of the cell wall was A1α linked through l-lysine as the diamino acid.
30328805	7	24	theme	peptidoglycan	1056:1068	arg1	type					1070:1073	The peptidoglycan type	1052:1073	The peptidoglycan type of the cell wall	1052:1090	The peptidoglycan type of the cell wall was A1α linked through l-lysine as the diamino acid.
30328805	4	25	theme	1297T	579:583	arg1	it					432:433	it	432:433	it	432:433	Phylogenetic analysis based on 16S rRNA gene sequences identified it as a member of the genus Jeotgalibacillus, most similar to Jeotgalibacillus campisalis SF-57T (98.7 % similarity), Jeotgalibacillus marinus DSM 1297T (98.2 %) and Jeotgalibacillus soli P9T (97.1 %).
30328805	4	25	theme	1297T	579:583	arg1	member					440:445	a member	438:445	a member of the genus Jeotgalibacillus, most similar to Jeotgalibacillus campisalis SF-57T (98.7 % similarity), Jeotgalibacillus marinus DSM 1297T (98.2 %)	438:592	Phylogenetic analysis based on 16S rRNA gene sequences identified it as a member of the genus Jeotgalibacillus, most similar to Jeotgalibacillus campisalis SF-57T (98.7 % similarity), Jeotgalibacillus marinus DSM 1297T (98.2 %) and Jeotgalibacillus soli P9T (97.1 %).
30328805	3	26	theme	2 	350:351	arg1	%					352:352	%	352:352	%	352:352	Optimal growth was observed at 33 °C on a 2216E agar plate of pH 7.5 and with 2 % (w/v) NaCl.
30328805	3	27	located	observed	291:298	arg1	plate					325:329	a 2216E agar plate	312:329	a 2216E agar plate of pH 7.5	312:339	Optimal growth was observed at 33 °C on a 2216E agar plate of pH 7.5 and with 2 % (w/v) NaCl.
30328805	3	27	located	observed	291:298	arg1	33 °C					303:307	33 °C	303:307	33 °C	303:307	Optimal growth was observed at 33 °C on a 2216E agar plate of pH 7.5 and with 2 % (w/v) NaCl.
30328805	3	27	located	observed	291:298	arg2	growth					280:285	Optimal growth	272:285	Optimal growth	272:285	Optimal growth was observed at 33 °C on a 2216E agar plate of pH 7.5 and with 2 % (w/v) NaCl.
30328805	10	28	theme	=MCCC	1396:1400	arg1	33930T					1416:1421	=MCCC 1H00228T=KCTC 33930T	1396:1421	=MCCC 1H00228T=KCTC 33930T	1396:1421	The type strain is 22-7T(=MCCC 1H00228T=KCTC 33930T).
30328805	10	28	theme	=MCCC	1396:1400	arg1	22-7T					1390:1394	22-7T	1390:1394	22-7T(=MCCC 1H00228T=KCTC 33930T)	1390:1422	The type strain is 22-7T(=MCCC 1H00228T=KCTC 33930T).
30328805	4	29	dep	Jeotgalibacillus	550:565	arg1	marinus					567:573	marinus	567:573	marinus	567:573	Phylogenetic analysis based on 16S rRNA gene sequences identified it as a member of the genus Jeotgalibacillus, most similar to Jeotgalibacillus campisalis SF-57T (98.7 % similarity), Jeotgalibacillus marinus DSM 1297T (98.2 %) and Jeotgalibacillus soli P9T (97.1 %).
30328805	4	30	theme	Jeotgalibacillus	494:509	arg1	SF-57T					522:527	Jeotgalibacillus campisalis SF-57T	494:527	Jeotgalibacillus campisalis SF-57T	494:527	Phylogenetic analysis based on 16S rRNA gene sequences identified it as a member of the genus Jeotgalibacillus, most similar to Jeotgalibacillus campisalis SF-57T (98.7 % similarity), Jeotgalibacillus marinus DSM 1297T (98.2 %) and Jeotgalibacillus soli P9T (97.1 %).
30328805	3	31	theme	Optimal	272:278	arg1	growth					280:285	Optimal growth	272:285	Optimal growth	272:285	Optimal growth was observed at 33 °C on a 2216E agar plate of pH 7.5 and with 2 % (w/v) NaCl.
30328805	6	32	theme	major	1024:1028	arg1	MK-7					1046:1049	MK-7	1046:1049	MK-7	1046:1049	The major polar lipids were aminophospholipid, phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol; the major fatty acids (>10 %) were anteiso-C15 : 0 and iso-C15 : 0; and the major menaquinone was MK-7.
30328805	6	32	theme	major	1024:1028	arg1	menaquinone					1030:1040	the major menaquinone	1020:1040	the major menaquinone	1020:1040	The major polar lipids were aminophospholipid, phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol; the major fatty acids (>10 %) were anteiso-C15 : 0 and iso-C15 : 0; and the major menaquinone was MK-7.
30328805	5	33	theme	hybridization	689:701	arg1	values					703:708	digital DNA-DNA hybridization values	673:708	digital DNA-DNA hybridization values	673:708	Average nucleotide identity values and digital DNA-DNA hybridization values were less than 74.2 and 18.1 %, respectively, between strain 22-7T and the type strains of closely related species.
30328805	2	34	theme	ocean	180:184	arg1	sediment					186:193	ocean sediment	180:193	ocean sediment of Laizhou Bay, China	180:215	A Gram-stain-positive, rod-shaped bacterial strain, 22-7T, was isolated from ocean sediment of Laizhou Bay, China, and was characterized by using a polyphasic approach.
30328805	8	35	theme	chemotaxonomic	1176:1189	arg1	characterizations					1205:1221	phenotypic, chemotaxonomic and genotypic characterizations	1164:1221	phenotypic, chemotaxonomic and genotypic characterizations	1164:1221	Combined data from phenotypic, chemotaxonomic and genotypic characterizations demonstrated that strain 22-7T represents a novel Jeotgalibacillus species, for which the name Jeotgalibacillus proteolyticus sp.
30328805	2	36	attach	isolated	166:173	arg2	22-7T					155:159	22-7T	155:159	22-7T	155:159	A Gram-stain-positive, rod-shaped bacterial strain, 22-7T, was isolated from ocean sediment of Laizhou Bay, China, and was characterized by using a polyphasic approach.
30328805	2	36	attach	isolated	166:173	arg1	sediment					186:193	ocean sediment	180:193	ocean sediment of Laizhou Bay, China	180:215	A Gram-stain-positive, rod-shaped bacterial strain, 22-7T, was isolated from ocean sediment of Laizhou Bay, China, and was characterized by using a polyphasic approach.
30328805	2	36	attach	isolated	166:173	arg2	strain					147:152	A Gram-stain-positive, rod-shaped bacterial strain	103:152	A Gram-stain-positive, rod-shaped bacterial strain	103:152	A Gram-stain-positive, rod-shaped bacterial strain, 22-7T, was isolated from ocean sediment of Laizhou Bay, China, and was characterized by using a polyphasic approach.
30328805	2	37	theme	Gram-stain-positive	105:123	arg1	22-7T					155:159	22-7T	155:159	22-7T	155:159	A Gram-stain-positive, rod-shaped bacterial strain, 22-7T, was isolated from ocean sediment of Laizhou Bay, China, and was characterized by using a polyphasic approach.
30328805	2	37	theme	Gram-stain-positive	105:123	arg1	strain					147:152	A Gram-stain-positive, rod-shaped bacterial strain	103:152	A Gram-stain-positive, rod-shaped bacterial strain	103:152	A Gram-stain-positive, rod-shaped bacterial strain, 22-7T, was isolated from ocean sediment of Laizhou Bay, China, and was characterized by using a polyphasic approach.
30328805	4	38	theme	rRNA	401:404	arg1	sequences					411:419	16S rRNA gene sequences	397:419	16S rRNA gene sequences	397:419	Phylogenetic analysis based on 16S rRNA gene sequences identified it as a member of the genus Jeotgalibacillus, most similar to Jeotgalibacillus campisalis SF-57T (98.7 % similarity), Jeotgalibacillus marinus DSM 1297T (98.2 %) and Jeotgalibacillus soli P9T (97.1 %).
30328805	8	39	dep	Jeotgalibacillus	1318:1333	arg1	proteolyticus					1335:1347	proteolyticus	1335:1347	proteolyticus	1335:1347	Combined data from phenotypic, chemotaxonomic and genotypic characterizations demonstrated that strain 22-7T represents a novel Jeotgalibacillus species, for which the name Jeotgalibacillus proteolyticus sp.
30328805	4	40	theme	DSM	575:577	arg1	1297T					579:583	the genus Jeotgalibacillus, most similar to Jeotgalibacillus campisalis SF-57T (98.7 % similarity), Jeotgalibacillus marinus DSM 1297T	450:583	the genus Jeotgalibacillus, most similar to Jeotgalibacillus campisalis SF-57T (98.7 % similarity), Jeotgalibacillus marinus DSM 1297T (98.2 %)	450:592	Phylogenetic analysis based on 16S rRNA gene sequences identified it as a member of the genus Jeotgalibacillus, most similar to Jeotgalibacillus campisalis SF-57T (98.7 % similarity), Jeotgalibacillus marinus DSM 1297T (98.2 %) and Jeotgalibacillus soli P9T (97.1 %).
30328805	4	40	theme	DSM	575:577	arg1	%					591:591	98.2 %	586:591	98.2 %	586:591	Phylogenetic analysis based on 16S rRNA gene sequences identified it as a member of the genus Jeotgalibacillus, most similar to Jeotgalibacillus campisalis SF-57T (98.7 % similarity), Jeotgalibacillus marinus DSM 1297T (98.2 %) and Jeotgalibacillus soli P9T (97.1 %).
30328805	3	41	theme	%	352:352	arg1	NaCl					360:363	2 % (w/v) NaCl	350:363	2 % (w/v) NaCl	350:363	Optimal growth was observed at 33 °C on a 2216E agar plate of pH 7.5 and with 2 % (w/v) NaCl.
30328805	4	42	theme	genus	454:458	arg1	1297T					579:583	the genus Jeotgalibacillus, most similar to Jeotgalibacillus campisalis SF-57T (98.7 % similarity), Jeotgalibacillus marinus DSM 1297T	450:583	the genus Jeotgalibacillus, most similar to Jeotgalibacillus campisalis SF-57T (98.7 % similarity), Jeotgalibacillus marinus DSM 1297T (98.2 %)	450:592	Phylogenetic analysis based on 16S rRNA gene sequences identified it as a member of the genus Jeotgalibacillus, most similar to Jeotgalibacillus campisalis SF-57T (98.7 % similarity), Jeotgalibacillus marinus DSM 1297T (98.2 %) and Jeotgalibacillus soli P9T (97.1 %).
30328805	4	42	theme	genus	454:458	arg1	%					591:591	98.2 %	586:591	98.2 %	586:591	Phylogenetic analysis based on 16S rRNA gene sequences identified it as a member of the genus Jeotgalibacillus, most similar to Jeotgalibacillus campisalis SF-57T (98.7 % similarity), Jeotgalibacillus marinus DSM 1297T (98.2 %) and Jeotgalibacillus soli P9T (97.1 %).
30328805	4	43	theme	16S	397:399	arg1	sequences					411:419	16S rRNA gene sequences	397:419	16S rRNA gene sequences	397:419	Phylogenetic analysis based on 16S rRNA gene sequences identified it as a member of the genus Jeotgalibacillus, most similar to Jeotgalibacillus campisalis SF-57T (98.7 % similarity), Jeotgalibacillus marinus DSM 1297T (98.2 %) and Jeotgalibacillus soli P9T (97.1 %).
30328805	8	44	theme	phenotypic	1164:1173	arg1	characterizations					1205:1221	phenotypic, chemotaxonomic and genotypic characterizations	1164:1221	phenotypic, chemotaxonomic and genotypic characterizations	1164:1221	Combined data from phenotypic, chemotaxonomic and genotypic characterizations demonstrated that strain 22-7T represents a novel Jeotgalibacillus species, for which the name Jeotgalibacillus proteolyticus sp.
30328805	6	45	theme	major	830:834	arg1	aminophospholipid					854:870	aminophospholipid	854:870	aminophospholipid	854:870	The major polar lipids were aminophospholipid, phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol; the major fatty acids (>10 %) were anteiso-C15 : 0 and iso-C15 : 0; and the major menaquinone was MK-7.
30328805	6	45	theme	major	830:834	arg1	lipids					842:847	The major polar lipids	826:847	The major polar lipids	826:847	The major polar lipids were aminophospholipid, phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol; the major fatty acids (>10 %) were anteiso-C15 : 0 and iso-C15 : 0; and the major menaquinone was MK-7.
30328805	6	45	theme	major	830:834	arg1	diphosphatidylglycerol					924:945	diphosphatidylglycerol	924:945	diphosphatidylglycerol	924:945	The major polar lipids were aminophospholipid, phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol; the major fatty acids (>10 %) were anteiso-C15 : 0 and iso-C15 : 0; and the major menaquinone was MK-7.
30328805	6	45	theme	major	830:834	arg1	phosphatidylglycerol					899:918	phosphatidylglycerol	899:918	phosphatidylglycerol	899:918	The major polar lipids were aminophospholipid, phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol; the major fatty acids (>10 %) were anteiso-C15 : 0 and iso-C15 : 0; and the major menaquinone was MK-7.
30328805	6	45	theme	major	830:834	arg1	phosphatidylethanolamine					873:896	phosphatidylethanolamine	873:896	phosphatidylethanolamine	873:896	The major polar lipids were aminophospholipid, phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol; the major fatty acids (>10 %) were anteiso-C15 : 0 and iso-C15 : 0; and the major menaquinone was MK-7.
30328805	6	46	dep	aminophospholipid	854:870	arg1	lipids					842:847	The major polar lipids	826:847	The major polar lipids	826:847	The major polar lipids were aminophospholipid, phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol; the major fatty acids (>10 %) were anteiso-C15 : 0 and iso-C15 : 0; and the major menaquinone was MK-7.
30328805	6	46	dep	aminophospholipid	854:870	arg1	aminophospholipid					854:870	aminophospholipid	854:870	aminophospholipid	854:870	The major polar lipids were aminophospholipid, phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol; the major fatty acids (>10 %) were anteiso-C15 : 0 and iso-C15 : 0; and the major menaquinone was MK-7.
30328805	6	46	dep	aminophospholipid	854:870	arg1	diphosphatidylglycerol					924:945	diphosphatidylglycerol	924:945	diphosphatidylglycerol	924:945	The major polar lipids were aminophospholipid, phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol; the major fatty acids (>10 %) were anteiso-C15 : 0 and iso-C15 : 0; and the major menaquinone was MK-7.
30328805	6	46	dep	aminophospholipid	854:870	arg1	phosphatidylglycerol					899:918	phosphatidylglycerol	899:918	phosphatidylglycerol	899:918	The major polar lipids were aminophospholipid, phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol; the major fatty acids (>10 %) were anteiso-C15 : 0 and iso-C15 : 0; and the major menaquinone was MK-7.
30328805	6	46	dep	aminophospholipid	854:870	arg1	phosphatidylethanolamine					873:896	phosphatidylethanolamine	873:896	phosphatidylethanolamine	873:896	The major polar lipids were aminophospholipid, phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol; the major fatty acids (>10 %) were anteiso-C15 : 0 and iso-C15 : 0; and the major menaquinone was MK-7.
30328805	2	47	theme	China	211:215	arg1	sediment					186:193	ocean sediment	180:193	ocean sediment of Laizhou Bay, China	180:215	A Gram-stain-positive, rod-shaped bacterial strain, 22-7T, was isolated from ocean sediment of Laizhou Bay, China, and was characterized by using a polyphasic approach.
30328805	7	48	theme	wall	1087:1090	arg1	acid					1139:1142	the diamino acid	1127:1142	the diamino acid	1127:1142	The peptidoglycan type of the cell wall was A1α linked through l-lysine as the diamino acid.
30328805	7	48	theme	wall	1087:1090	arg1	type					1070:1073	The peptidoglycan type	1052:1073	The peptidoglycan type of the cell wall	1052:1090	The peptidoglycan type of the cell wall was A1α linked through l-lysine as the diamino acid.
30328805	5	49	theme	related	809:815	arg1	species					817:823	closely related species	801:823	closely related species	801:823	Average nucleotide identity values and digital DNA-DNA hybridization values were less than 74.2 and 18.1 %, respectively, between strain 22-7T and the type strains of closely related species.
30328805	8	50	theme	Jeotgalibacillus	1318:1333	arg1	sp					1349:1350	the name Jeotgalibacillus proteolyticus sp	1309:1350	the name Jeotgalibacillus proteolyticus sp	1309:1350	Combined data from phenotypic, chemotaxonomic and genotypic characterizations demonstrated that strain 22-7T represents a novel Jeotgalibacillus species, for which the name Jeotgalibacillus proteolyticus sp.
30328805	6	51	theme	major	952:956	arg1	acids					964:968	the major fatty acids	948:968	the major fatty acids (>10 %)	948:976	The major polar lipids were aminophospholipid, phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol; the major fatty acids (>10 %) were anteiso-C15 : 0 and iso-C15 : 0; and the major menaquinone was MK-7.
30328805	6	51	theme	major	952:956	arg1	%					975:975	>10 %	971:975	>10 %	971:975	The major polar lipids were aminophospholipid, phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol; the major fatty acids (>10 %) were anteiso-C15 : 0 and iso-C15 : 0; and the major menaquinone was MK-7.
30328805	6	51	theme	major	952:956	arg1	anteiso-C15 					983:994	anteiso-C15 	983:994	anteiso-C15 	983:994	The major polar lipids were aminophospholipid, phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol; the major fatty acids (>10 %) were anteiso-C15 : 0 and iso-C15 : 0; and the major menaquinone was MK-7.
30328805	5	52	theme	species	817:823	arg1	strains					790:796	the type strains	781:796	the type strains of closely related species	781:823	Average nucleotide identity values and digital DNA-DNA hybridization values were less than 74.2 and 18.1 %, respectively, between strain 22-7T and the type strains of closely related species.
30328805	5	52	theme	species	817:823	arg1	strain					764:769	strain 22-7T	764:775	strain 22-7T	764:775	Average nucleotide identity values and digital DNA-DNA hybridization values were less than 74.2 and 18.1 %, respectively, between strain 22-7T and the type strains of closely related species.
30328805	6	53	theme	fatty	958:962	arg1	acids					964:968	the major fatty acids	948:968	the major fatty acids (>10 %)	948:976	The major polar lipids were aminophospholipid, phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol; the major fatty acids (>10 %) were anteiso-C15 : 0 and iso-C15 : 0; and the major menaquinone was MK-7.
30328805	6	53	theme	fatty	958:962	arg1	%					975:975	>10 %	971:975	>10 %	971:975	The major polar lipids were aminophospholipid, phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol; the major fatty acids (>10 %) were anteiso-C15 : 0 and iso-C15 : 0; and the major menaquinone was MK-7.
30328805	6	53	theme	fatty	958:962	arg1	anteiso-C15 					983:994	anteiso-C15 	983:994	anteiso-C15 	983:994	The major polar lipids were aminophospholipid, phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol; the major fatty acids (>10 %) were anteiso-C15 : 0 and iso-C15 : 0; and the major menaquinone was MK-7.
30328805	2	54	theme	polyphasic	251:260	arg1	approach					262:269	a polyphasic approach	249:269	a polyphasic approach	249:269	A Gram-stain-positive, rod-shaped bacterial strain, 22-7T, was isolated from ocean sediment of Laizhou Bay, China, and was characterized by using a polyphasic approach.
30328805	8	55	theme	name	1313:1316	arg1	sp					1349:1350	the name Jeotgalibacillus proteolyticus sp	1309:1350	the name Jeotgalibacillus proteolyticus sp	1309:1350	Combined data from phenotypic, chemotaxonomic and genotypic characterizations demonstrated that strain 22-7T represents a novel Jeotgalibacillus species, for which the name Jeotgalibacillus proteolyticus sp.
30328805	7	56	theme	diamino	1131:1137	arg1	acid					1139:1142	the diamino acid	1127:1142	the diamino acid	1127:1142	The peptidoglycan type of the cell wall was A1α linked through l-lysine as the diamino acid.
30328805	7	56	theme	diamino	1131:1137	arg1	type					1070:1073	The peptidoglycan type	1052:1073	The peptidoglycan type of the cell wall	1052:1090	The peptidoglycan type of the cell wall was A1α linked through l-lysine as the diamino acid.
30328805	4	57	theme	%	535:535	arg1	similarity					537:546	98.7 % similarity	530:546	98.7 % similarity	530:546	Phylogenetic analysis based on 16S rRNA gene sequences identified it as a member of the genus Jeotgalibacillus, most similar to Jeotgalibacillus campisalis SF-57T (98.7 % similarity), Jeotgalibacillus marinus DSM 1297T (98.2 %) and Jeotgalibacillus soli P9T (97.1 %).
30328805	4	58	theme	Phylogenetic	366:377	arg1	analysis					379:386	Phylogenetic analysis	366:386	Phylogenetic analysis based on 16S rRNA gene sequences	366:419	Phylogenetic analysis based on 16S rRNA gene sequences identified it as a member of the genus Jeotgalibacillus, most similar to Jeotgalibacillus campisalis SF-57T (98.7 % similarity), Jeotgalibacillus marinus DSM 1297T (98.2 %) and Jeotgalibacillus soli P9T (97.1 %).
30328805	8	59	theme	Combined	1145:1152	arg1	data					1154:1157	Combined data	1145:1157	Combined data from phenotypic, chemotaxonomic and genotypic characterizations	1145:1221	Combined data from phenotypic, chemotaxonomic and genotypic characterizations demonstrated that strain 22-7T represents a novel Jeotgalibacillus species, for which the name Jeotgalibacillus proteolyticus sp.
30328805	8	60	theme	genotypic	1195:1203	arg1	characterizations					1205:1221	phenotypic, chemotaxonomic and genotypic characterizations	1164:1221	phenotypic, chemotaxonomic and genotypic characterizations	1164:1221	Combined data from phenotypic, chemotaxonomic and genotypic characterizations demonstrated that strain 22-7T represents a novel Jeotgalibacillus species, for which the name Jeotgalibacillus proteolyticus sp.
30328805	3	61	theme	2216E	314:318	arg1	plate					325:329	a 2216E agar plate	312:329	a 2216E agar plate of pH 7.5	312:339	Optimal growth was observed at 33 °C on a 2216E agar plate of pH 7.5 and with 2 % (w/v) NaCl.
30328805	1	62	theme	protease-producing	43:60	arg1	bacterium					62:70	a protease-producing bacterium	41:70	a protease-producing bacterium	41:70	nov., a protease-producing bacterium isolated from ocean sediments.
30328805	1	62	theme	protease-producing	43:60	arg1	nov.					35:38	nov.	35:38	nov.	35:38	nov., a protease-producing bacterium isolated from ocean sediments.
30328805	5	63	theme	type	785:788	arg1	strains					790:796	the type strains	781:796	the type strains of closely related species	781:823	Average nucleotide identity values and digital DNA-DNA hybridization values were less than 74.2 and 18.1 %, respectively, between strain 22-7T and the type strains of closely related species.
30328805	6	64	theme	polar	836:840	arg1	aminophospholipid					854:870	aminophospholipid	854:870	aminophospholipid	854:870	The major polar lipids were aminophospholipid, phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol; the major fatty acids (>10 %) were anteiso-C15 : 0 and iso-C15 : 0; and the major menaquinone was MK-7.
30328805	6	64	theme	polar	836:840	arg1	lipids					842:847	The major polar lipids	826:847	The major polar lipids	826:847	The major polar lipids were aminophospholipid, phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol; the major fatty acids (>10 %) were anteiso-C15 : 0 and iso-C15 : 0; and the major menaquinone was MK-7.
30328805	6	64	theme	polar	836:840	arg1	diphosphatidylglycerol					924:945	diphosphatidylglycerol	924:945	diphosphatidylglycerol	924:945	The major polar lipids were aminophospholipid, phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol; the major fatty acids (>10 %) were anteiso-C15 : 0 and iso-C15 : 0; and the major menaquinone was MK-7.
30328805	6	64	theme	polar	836:840	arg1	phosphatidylglycerol					899:918	phosphatidylglycerol	899:918	phosphatidylglycerol	899:918	The major polar lipids were aminophospholipid, phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol; the major fatty acids (>10 %) were anteiso-C15 : 0 and iso-C15 : 0; and the major menaquinone was MK-7.
30328805	6	64	theme	polar	836:840	arg1	phosphatidylethanolamine					873:896	phosphatidylethanolamine	873:896	phosphatidylethanolamine	873:896	The major polar lipids were aminophospholipid, phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol; the major fatty acids (>10 %) were anteiso-C15 : 0 and iso-C15 : 0; and the major menaquinone was MK-7.
30328805	5	65	theme	Average	634:640	arg1	values					662:667	Average nucleotide identity values	634:667	Average nucleotide identity values	634:667	Average nucleotide identity values and digital DNA-DNA hybridization values were less than 74.2 and 18.1 %, respectively, between strain 22-7T and the type strains of closely related species.
30010524	11	0	theme	novel	1498:1502	arg1	species					1504:1510	novel species	1498:1510	a novel species Frankia irregularis sp	1496:1533	On the basis of these results, strain G2T (=DSM 45899T=CECT 9038T) is proposed to represent the type strain of a novel species Frankia irregularis sp.
30010524	10	1	theme	Frankia	1334:1340	arg1	discariaewere					1342:1354	Frankia discariaewere	1334:1354	Frankia discariaewere	1334:1354	Digital DNA -DNA hybridisation (dDDH) values between G2T and its nearest phylogenetic neighbours Frankia elaeagniand Frankia discariaewere below the threshold of 70 %.
30010524	10	2	theme	DNA -DNA	1225:1232	arg1	values					1255:1260	Digital DNA -DNA hybridisation (dDDH) values	1217:1260	Digital DNA -DNA hybridisation (dDDH) values between G2T and its nearest phylogenetic neighbours Frankia	1217:1320	Digital DNA -DNA hybridisation (dDDH) values between G2T and its nearest phylogenetic neighbours Frankia elaeagniand Frankia discariaewere below the threshold of 70 %.
30010524	11	3	theme	sp	1532:1533	arg1	strain					1486:1491	the type strain	1477:1491	the type strain of a novel species Frankia irregularis sp	1477:1533	On the basis of these results, strain G2T (=DSM 45899T=CECT 9038T) is proposed to represent the type strain of a novel species Frankia irregularis sp.
30010524	11	4	theme	irregularis	1520:1530	arg1	sp					1532:1533	a novel species Frankia irregularis sp	1496:1533	a novel species Frankia irregularis sp	1496:1533	On the basis of these results, strain G2T (=DSM 45899T=CECT 9038T) is proposed to represent the type strain of a novel species Frankia irregularis sp.
30010524	8	5	theme	genome	1042:1047	arg1	9.5 Mb					1060:1065	9.5 Mb	1060:1065	9.5 Mb	1060:1065	The size of the genome of G2T was 9.5 Mb and digital DNA G+C content was 70.9 %.
30010524	8	5	theme	genome	1042:1047	arg1	size					1030:1033	The size	1026:1033	The size of the genome of G2T	1026:1054	The size of the genome of G2T was 9.5 Mb and digital DNA G+C content was 70.9 %.
30010524	9	6	theme	type	1171:1174	arg1	strains					1176:1182	the type strains	1167:1182	the type strains of species of the genus Frankia	1167:1214	The 16S rRNA gene showed 97.4-99.5 % sequence identity with the type strains of species of the genus Frankia.
30010524	9	7	theme	 sequence	1143:1151	arg1	identity					1153:1160	97.4-99.5 % sequence identity	1132:1160	97.4-99.5 % sequence identity with the type strains of species of the genus Frankia	1132:1214	The 16S rRNA gene showed 97.4-99.5 % sequence identity with the type strains of species of the genus Frankia.
30010524	10	8	dep	G2T	1270:1272	arg1	Frankia					1314:1320	Frankia	1314:1320	Frankia	1314:1320	Digital DNA -DNA hybridisation (dDDH) values between G2T and its nearest phylogenetic neighbours Frankia elaeagniand Frankia discariaewere below the threshold of 70 %.
30010524	4	9	theme	polyphasic	526:535	arg1	approach					537:544	a polyphasic approach	524:544	a polyphasic approach	524:544	The taxonomic position of G2T was determined using a polyphasic approach.
30010524	11	10	theme	strain	1416:1421	arg1	9038T					1445:1449	=DSM 45899T=CECT 9038T	1428:1449	=DSM 45899T=CECT 9038T	1428:1449	On the basis of these results, strain G2T (=DSM 45899T=CECT 9038T) is proposed to represent the type strain of a novel species Frankia irregularis sp.
30010524	11	10	theme	strain	1416:1421	arg1	G2T					1423:1425	strain G2T	1416:1425	strain G2T (=DSM 45899T=CECT 9038T)	1416:1450	On the basis of these results, strain G2T (=DSM 45899T=CECT 9038T) is proposed to represent the type strain of a novel species Frankia irregularis sp.
30010524	1	11	theme	unable	49:54	arg1	nov.					24:27	nov.	24:27	nov.	24:27	nov., an actinobacterium unable to nodulate its original host, Casuarina equisetifolia, but effectively nodulates members of the actinorhizal Rhamnales.
30010524	1	11	theme	unable	49:54	arg1	actinobacterium					33:47	an actinobacterium	30:47	an actinobacterium unable to nodulate its original host	30:84	nov., an actinobacterium unable to nodulate its original host, Casuarina equisetifolia, but effectively nodulates members of the actinorhizal Rhamnales.
30010524	8	12	theme	G2T	1052:1054	arg1	genome					1042:1047	the genome	1038:1047	the genome of G2T	1038:1054	The size of the genome of G2T was 9.5 Mb and digital DNA G+C content was 70.9 %.
30010524	4	13	theme	G2T	499:501	arg1	position					487:494	The taxonomic position	473:494	The taxonomic position of G2T	473:501	The taxonomic position of G2T was determined using a polyphasic approach.
30010524	7	14	theme	fatty	972:976	arg1	iso-C16 					989:996	iso-C16 	989:996	iso-C16 	989:996	The predominant menaquinones were MK-9 (H4) and MK-9 (H6) while the major fatty acids were iso-C16 : 0, C17 : 1ω8c and C15 : 0.
30010524	7	14	theme	fatty	972:976	arg1	acids					978:982	the major fatty acids	962:982	the major fatty acids	962:982	The predominant menaquinones were MK-9 (H4) and MK-9 (H6) while the major fatty acids were iso-C16 : 0, C17 : 1ω8c and C15 : 0.
30010524	2	15	theme	mutilocular	284:294	arg1	sporangia					296:304	mutilocular sporangia	284:304	mutilocular sporangia	284:304	A red pigmented actinobacterium designated G2T, forming extremely branched vegetative hyphae, vesicles and mutilocular sporangia, was isolated from Casuarina equisetifolia nodules.
30010524	2	15	theme	mutilocular	284:294	arg1	actinobacterium					193:207	A red pigmented actinobacterium	177:207	A red pigmented actinobacterium designated G2T	177:222	A red pigmented actinobacterium designated G2T, forming extremely branched vegetative hyphae, vesicles and mutilocular sporangia, was isolated from Casuarina equisetifolia nodules.
30010524	11	16	theme	=DSM	1428:1431	arg1	9038T					1445:1449	=DSM 45899T=CECT 9038T	1428:1449	=DSM 45899T=CECT 9038T	1428:1449	On the basis of these results, strain G2T (=DSM 45899T=CECT 9038T) is proposed to represent the type strain of a novel species Frankia irregularis sp.
30010524	11	16	theme	=DSM	1428:1431	arg1	G2T					1423:1425	strain G2T	1416:1425	strain G2T (=DSM 45899T=CECT 9038T)	1416:1450	On the basis of these results, strain G2T (=DSM 45899T=CECT 9038T) is proposed to represent the type strain of a novel species Frankia irregularis sp.
30010524	7	17	dep	iso-C16 	989:996	arg1	C17 					1002:1005	C17 	1002:1005	C17 	1002:1005	The predominant menaquinones were MK-9 (H4) and MK-9 (H6) while the major fatty acids were iso-C16 : 0, C17 : 1ω8c and C15 : 0.
30010524	7	17	dep	iso-C16 	989:996	arg1	 0					998:999	 0	998:999	 0	998:999	The predominant menaquinones were MK-9 (H4) and MK-9 (H6) while the major fatty acids were iso-C16 : 0, C17 : 1ω8c and C15 : 0.
30010524	9	18	theme	species	1187:1193	arg1	strains					1176:1182	the type strains	1167:1182	the type strains of species of the genus Frankia	1167:1214	The 16S rRNA gene showed 97.4-99.5 % sequence identity with the type strains of species of the genus Frankia.
30010524	11	19	theme	species	1504:1510	arg1	sp					1532:1533	a novel species Frankia irregularis sp	1496:1533	a novel species Frankia irregularis sp	1496:1533	On the basis of these results, strain G2T (=DSM 45899T=CECT 9038T) is proposed to represent the type strain of a novel species Frankia irregularis sp.
30010524	9	20	with	identity	1153:1160	arg1	strains					1176:1182	the type strains	1167:1182	the type strains of species of the genus Frankia	1167:1214	The 16S rRNA gene showed 97.4-99.5 % sequence identity with the type strains of species of the genus Frankia.
30010524	1	21	theme	actinorhizal	153:164	arg1	Rhamnales					166:174	the actinorhizal Rhamnales	149:174	the actinorhizal Rhamnales	149:174	nov., an actinobacterium unable to nodulate its original host, Casuarina equisetifolia, but effectively nodulates members of the actinorhizal Rhamnales.
30010524	6	22	theme	unknown	878:884	arg1	lipids					886:891	unknown lipids	878:891	unknown lipids (L)	878:895	The polar lipid pattern consisted of phosphatidylinositol (PI), diphosphatidylglycerol (DPG), glycophospholipids (GPL1-2), phosphatidylglycerol (PG), aminophospholipid (APL) and unknown lipids (L).
30010524	6	22	theme	unknown	878:884	arg1	L					894:894	L	894:894	L	894:894	The polar lipid pattern consisted of phosphatidylinositol (PI), diphosphatidylglycerol (DPG), glycophospholipids (GPL1-2), phosphatidylglycerol (PG), aminophospholipid (APL) and unknown lipids (L).
30010524	10	23	theme	phylogenetic	1290:1301	arg1	neighbours					1303:1312	its nearest phylogenetic neighbours	1278:1312	its nearest phylogenetic neighbours	1278:1312	Digital DNA -DNA hybridisation (dDDH) values between G2T and its nearest phylogenetic neighbours Frankia elaeagniand Frankia discariaewere below the threshold of 70 %.
30010524	0	24	theme	Frankia	0:6	arg1	sp					20:21	Frankia irregularis sp	0:21	Frankia irregularis sp.	0:22	Frankia irregularis sp.
30010524	1	25	theme	Rhamnales	166:174	arg1	members					138:144	members	138:144	members of the actinorhizal Rhamnales	138:174	nov., an actinobacterium unable to nodulate its original host, Casuarina equisetifolia, but effectively nodulates members of the actinorhizal Rhamnales.
30010524	0	26	theme	irregularis	8:18	arg1	sp					20:21	Frankia irregularis sp	0:21	Frankia irregularis sp.	0:22	Frankia irregularis sp.
30010524	11	27	theme	Frankia	1512:1518	arg1	sp					1532:1533	a novel species Frankia irregularis sp	1496:1533	a novel species Frankia irregularis sp	1496:1533	On the basis of these results, strain G2T (=DSM 45899T=CECT 9038T) is proposed to represent the type strain of a novel species Frankia irregularis sp.
30010524	9	28	theme	genus	1202:1206	arg1	Frankia					1208:1214	the genus Frankia	1198:1214	the genus Frankia	1198:1214	The 16S rRNA gene showed 97.4-99.5 % sequence identity with the type strains of species of the genus Frankia.
30010524	1	29	theme	original	72:79	arg1	host					81:84	its original host	68:84	its original host	68:84	nov., an actinobacterium unable to nodulate its original host, Casuarina equisetifolia, but effectively nodulates members of the actinorhizal Rhamnales.
30010524	6	30	theme	lipid	710:714	arg1	pattern					716:722	The polar lipid pattern	700:722	The polar lipid pattern	700:722	The polar lipid pattern consisted of phosphatidylinositol (PI), diphosphatidylglycerol (DPG), glycophospholipids (GPL1-2), phosphatidylglycerol (PG), aminophospholipid (APL) and unknown lipids (L).
30010524	3	31	theme	host	401:404	arg1	plant					406:410	its original host plant	388:410	its original host plant but effectively nodulated members of actinorhizal Rhamnales	388:470	The strain failed to nodulate its original host plant but effectively nodulated members of actinorhizal Rhamnales.
30010524	2	32	attach	isolated	311:318	arg1	nodules					349:355	Casuarina equisetifolia nodules	325:355	Casuarina equisetifolia nodules	325:355	A red pigmented actinobacterium designated G2T, forming extremely branched vegetative hyphae, vesicles and mutilocular sporangia, was isolated from Casuarina equisetifolia nodules.
30010524	2	32	attach	isolated	311:318	arg2	vesicles					271:278	vesicles	271:278	vesicles	271:278	A red pigmented actinobacterium designated G2T, forming extremely branched vegetative hyphae, vesicles and mutilocular sporangia, was isolated from Casuarina equisetifolia nodules.
30010524	2	32	attach	isolated	311:318	arg2	actinobacterium					193:207	A red pigmented actinobacterium	177:207	A red pigmented actinobacterium designated G2T	177:222	A red pigmented actinobacterium designated G2T, forming extremely branched vegetative hyphae, vesicles and mutilocular sporangia, was isolated from Casuarina equisetifolia nodules.
30010524	2	32	attach	isolated	311:318	arg2	sporangia					296:304	mutilocular sporangia	284:304	mutilocular sporangia	284:304	A red pigmented actinobacterium designated G2T, forming extremely branched vegetative hyphae, vesicles and mutilocular sporangia, was isolated from Casuarina equisetifolia nodules.
30010524	2	32	attach	isolated	311:318	arg2	hyphae					263:268	forming extremely branched vegetative hyphae	225:268	forming extremely branched vegetative hyphae	225:268	A red pigmented actinobacterium designated G2T, forming extremely branched vegetative hyphae, vesicles and mutilocular sporangia, was isolated from Casuarina equisetifolia nodules.
30010524	5	33	theme	diagnostic	617:626	arg1	acid					636:639	diagnostic diamino acid	617:639	diagnostic diamino acid	617:639	The peptidoglycan of the strain contained meso-diaminopimelic acid as diagnostic diamino acid, galactose, glucose, mannose, rhamnose, ribose and xylose.
30010524	11	34	theme	45899T=CECT	1433:1443	arg1	9038T					1445:1449	=DSM 45899T=CECT 9038T	1428:1449	=DSM 45899T=CECT 9038T	1428:1449	On the basis of these results, strain G2T (=DSM 45899T=CECT 9038T) is proposed to represent the type strain of a novel species Frankia irregularis sp.
30010524	11	34	theme	45899T=CECT	1433:1443	arg1	G2T					1423:1425	strain G2T	1416:1425	strain G2T (=DSM 45899T=CECT 9038T)	1416:1450	On the basis of these results, strain G2T (=DSM 45899T=CECT 9038T) is proposed to represent the type strain of a novel species Frankia irregularis sp.
30010524	6	35	theme	polar	704:708	arg1	pattern					716:722	The polar lipid pattern	700:722	The polar lipid pattern	700:722	The polar lipid pattern consisted of phosphatidylinositol (PI), diphosphatidylglycerol (DPG), glycophospholipids (GPL1-2), phosphatidylglycerol (PG), aminophospholipid (APL) and unknown lipids (L).
30010524	7	36	theme	predominant	902:912	arg1	MK-9					932:935	MK-9	932:935	MK-9	932:935	The predominant menaquinones were MK-9 (H4) and MK-9 (H6) while the major fatty acids were iso-C16 : 0, C17 : 1ω8c and C15 : 0.
30010524	7	36	theme	predominant	902:912	arg1	menaquinones					914:925	The predominant menaquinones	898:925	The predominant menaquinones	898:925	The predominant menaquinones were MK-9 (H4) and MK-9 (H6) while the major fatty acids were iso-C16 : 0, C17 : 1ω8c and C15 : 0.
30010524	5	37	theme	diamino	628:634	arg1	acid					636:639	diagnostic diamino acid	617:639	diagnostic diamino acid	617:639	The peptidoglycan of the strain contained meso-diaminopimelic acid as diagnostic diamino acid, galactose, glucose, mannose, rhamnose, ribose and xylose.
30010524	10	38	theme	%	1382:1382	arg1	threshold					1366:1374	the threshold	1362:1374	the threshold of 70 %	1362:1382	Digital DNA -DNA hybridisation (dDDH) values between G2T and its nearest phylogenetic neighbours Frankia elaeagniand Frankia discariaewere below the threshold of 70 %.
30010524	9	39	theme	16S	1111:1113	arg1	gene					1120:1123	The 16S rRNA gene	1107:1123	The 16S rRNA gene	1107:1123	The 16S rRNA gene showed 97.4-99.5 % sequence identity with the type strains of species of the genus Frankia.
30010524	1	40	theme	Casuarina	87:95	arg1	equisetifolia					97:109	Casuarina equisetifolia	87:109	Casuarina equisetifolia	87:109	nov., an actinobacterium unable to nodulate its original host, Casuarina equisetifolia, but effectively nodulates members of the actinorhizal Rhamnales.
30010524	1	40	theme	Casuarina	87:95	arg1	nov.					24:27	nov.	24:27	nov.	24:27	nov., an actinobacterium unable to nodulate its original host, Casuarina equisetifolia, but effectively nodulates members of the actinorhizal Rhamnales.
30010524	10	41	theme	nearest	1282:1288	arg1	neighbours					1303:1312	its nearest phylogenetic neighbours	1278:1312	its nearest phylogenetic neighbours	1278:1312	Digital DNA -DNA hybridisation (dDDH) values between G2T and its nearest phylogenetic neighbours Frankia elaeagniand Frankia discariaewere below the threshold of 70 %.
30010524	9	42	theme	rRNA	1115:1118	arg1	gene					1120:1123	The 16S rRNA gene	1107:1123	The 16S rRNA gene	1107:1123	The 16S rRNA gene showed 97.4-99.5 % sequence identity with the type strains of species of the genus Frankia.
30010524	9	43	theme	Frankia	1208:1214	arg1	species					1187:1193	species	1187:1193	species of the genus Frankia	1187:1214	The 16S rRNA gene showed 97.4-99.5 % sequence identity with the type strains of species of the genus Frankia.
30010524	3	44	theme	actinorhizal	449:460	arg1	Rhamnales					462:470	actinorhizal Rhamnales	449:470	actinorhizal Rhamnales	449:470	The strain failed to nodulate its original host plant but effectively nodulated members of actinorhizal Rhamnales.
30010524	3	45	theme	nodulated	428:436	arg1	members					438:444	nodulated members	428:444	its original host plant but effectively nodulated members of actinorhizal Rhamnales	388:470	The strain failed to nodulate its original host plant but effectively nodulated members of actinorhizal Rhamnales.
30010524	2	46	theme	forming	225:231	arg1	actinobacterium					193:207	A red pigmented actinobacterium	177:207	A red pigmented actinobacterium designated G2T	177:222	A red pigmented actinobacterium designated G2T, forming extremely branched vegetative hyphae, vesicles and mutilocular sporangia, was isolated from Casuarina equisetifolia nodules.
30010524	2	46	theme	forming	225:231	arg1	hyphae					263:268	forming extremely branched vegetative hyphae	225:268	forming extremely branched vegetative hyphae	225:268	A red pigmented actinobacterium designated G2T, forming extremely branched vegetative hyphae, vesicles and mutilocular sporangia, was isolated from Casuarina equisetifolia nodules.
30010524	7	47	theme	major	966:970	arg1	iso-C16 					989:996	iso-C16 	989:996	iso-C16 	989:996	The predominant menaquinones were MK-9 (H4) and MK-9 (H6) while the major fatty acids were iso-C16 : 0, C17 : 1ω8c and C15 : 0.
30010524	7	47	theme	major	966:970	arg1	acids					978:982	the major fatty acids	962:982	the major fatty acids	962:982	The predominant menaquinones were MK-9 (H4) and MK-9 (H6) while the major fatty acids were iso-C16 : 0, C17 : 1ω8c and C15 : 0.
30010524	8	48	theme	DNA	1079:1081	arg1	G+C content					1083:1093	digital DNA G+C content	1071:1093	digital DNA G+C content	1071:1093	The size of the genome of G2T was 9.5 Mb and digital DNA G+C content was 70.9 %.
30010524	4	49	theme	taxonomic	477:485	arg1	position					487:494	The taxonomic position	473:494	The taxonomic position of G2T	473:501	The taxonomic position of G2T was determined using a polyphasic approach.
30010524	5	50	theme	strain	572:577	arg1	peptidoglycan					551:563	The peptidoglycan	547:563	The peptidoglycan of the strain	547:577	The peptidoglycan of the strain contained meso-diaminopimelic acid as diagnostic diamino acid, galactose, glucose, mannose, rhamnose, ribose and xylose.
30010524	9	51	theme	97.4-99.5 	1132:1141	arg1	%					1142:1142	%	1142:1142	%	1142:1142	The 16S rRNA gene showed 97.4-99.5 % sequence identity with the type strains of species of the genus Frankia.
30010524	5	52	contain	contained	579:587	arg1	peptidoglycan					551:563	The peptidoglycan	547:563	The peptidoglycan of the strain	547:577	The peptidoglycan of the strain contained meso-diaminopimelic acid as diagnostic diamino acid, galactose, glucose, mannose, rhamnose, ribose and xylose.
30010524	5	52	contain	contained	579:587	arg2	acid					609:612	meso-diaminopimelic acid	589:612	meso-diaminopimelic acid	589:612	The peptidoglycan of the strain contained meso-diaminopimelic acid as diagnostic diamino acid, galactose, glucose, mannose, rhamnose, ribose and xylose.
30010524	9	53	theme	%	1142:1142	arg1	identity					1153:1160	97.4-99.5 % sequence identity	1132:1160	97.4-99.5 % sequence identity with the type strains of species of the genus Frankia	1132:1214	The 16S rRNA gene showed 97.4-99.5 % sequence identity with the type strains of species of the genus Frankia.
30010524	5	54	theme	meso-diaminopimelic	589:607	arg1	acid					609:612	meso-diaminopimelic acid	589:612	meso-diaminopimelic acid	589:612	The peptidoglycan of the strain contained meso-diaminopimelic acid as diagnostic diamino acid, galactose, glucose, mannose, rhamnose, ribose and xylose.
30010524	2	55	theme	equisetifolia	335:347	arg1	nodules					349:355	Casuarina equisetifolia nodules	325:355	Casuarina equisetifolia nodules	325:355	A red pigmented actinobacterium designated G2T, forming extremely branched vegetative hyphae, vesicles and mutilocular sporangia, was isolated from Casuarina equisetifolia nodules.
30010524	11	56	dep	results	1407:1413	arg1	the					1388:1390	the	1388:1390	the	1388:1390	On the basis of these results, strain G2T (=DSM 45899T=CECT 9038T) is proposed to represent the type strain of a novel species Frankia irregularis sp.
30010524	11	56	dep	results	1407:1413	arg1	basis					1392:1396	basis	1392:1396	basis	1392:1396	On the basis of these results, strain G2T (=DSM 45899T=CECT 9038T) is proposed to represent the type strain of a novel species Frankia irregularis sp.
30010524	2	57	theme	pigmented	183:191	arg1	hyphae					263:268	forming extremely branched vegetative hyphae	225:268	forming extremely branched vegetative hyphae	225:268	A red pigmented actinobacterium designated G2T, forming extremely branched vegetative hyphae, vesicles and mutilocular sporangia, was isolated from Casuarina equisetifolia nodules.
30010524	2	57	theme	pigmented	183:191	arg1	vesicles					271:278	vesicles	271:278	vesicles	271:278	A red pigmented actinobacterium designated G2T, forming extremely branched vegetative hyphae, vesicles and mutilocular sporangia, was isolated from Casuarina equisetifolia nodules.
30010524	2	57	theme	pigmented	183:191	arg1	sporangia					296:304	mutilocular sporangia	284:304	mutilocular sporangia	284:304	A red pigmented actinobacterium designated G2T, forming extremely branched vegetative hyphae, vesicles and mutilocular sporangia, was isolated from Casuarina equisetifolia nodules.
30010524	2	57	theme	pigmented	183:191	arg1	actinobacterium					193:207	A red pigmented actinobacterium	177:207	A red pigmented actinobacterium designated G2T	177:222	A red pigmented actinobacterium designated G2T, forming extremely branched vegetative hyphae, vesicles and mutilocular sporangia, was isolated from Casuarina equisetifolia nodules.
30010524	8	58	theme	digital	1071:1077	arg1	G+C content					1083:1093	digital DNA G+C content	1071:1093	digital DNA G+C content	1071:1093	The size of the genome of G2T was 9.5 Mb and digital DNA G+C content was 70.9 %.
30010524	10	59	theme	dDDH	1249:1252	arg1	values					1255:1260	Digital DNA -DNA hybridisation (dDDH) values	1217:1260	Digital DNA -DNA hybridisation (dDDH) values between G2T and its nearest phylogenetic neighbours Frankia	1217:1320	Digital DNA -DNA hybridisation (dDDH) values between G2T and its nearest phylogenetic neighbours Frankia elaeagniand Frankia discariaewere below the threshold of 70 %.
30010524	2	60	theme	Casuarina	325:333	arg1	nodules					349:355	Casuarina equisetifolia nodules	325:355	Casuarina equisetifolia nodules	325:355	A red pigmented actinobacterium designated G2T, forming extremely branched vegetative hyphae, vesicles and mutilocular sporangia, was isolated from Casuarina equisetifolia nodules.
30010524	10	61	theme	Digital	1217:1223	arg1	values					1255:1260	Digital DNA -DNA hybridisation (dDDH) values	1217:1260	Digital DNA -DNA hybridisation (dDDH) values between G2T and its nearest phylogenetic neighbours Frankia	1217:1320	Digital DNA -DNA hybridisation (dDDH) values between G2T and its nearest phylogenetic neighbours Frankia elaeagniand Frankia discariaewere below the threshold of 70 %.
30010524	7	62	dep	 0	998:999	arg1	C15 					1017:1020	C15 	1017:1020	C15 	1017:1020	The predominant menaquinones were MK-9 (H4) and MK-9 (H6) while the major fatty acids were iso-C16 : 0, C17 : 1ω8c and C15 : 0.
30010524	7	62	dep	 0	998:999	arg1	 1ω8c					1007:1011	 1ω8c	1007:1011	 1ω8c	1007:1011	The predominant menaquinones were MK-9 (H4) and MK-9 (H6) while the major fatty acids were iso-C16 : 0, C17 : 1ω8c and C15 : 0.
30010524	7	62	dep	 0	998:999	arg1	 0					1022:1023	 0	1022:1023	 0	1022:1023	The predominant menaquinones were MK-9 (H4) and MK-9 (H6) while the major fatty acids were iso-C16 : 0, C17 : 1ω8c and C15 : 0.
30010524	2	63	theme	red	179:181	arg1	hyphae					263:268	forming extremely branched vegetative hyphae	225:268	forming extremely branched vegetative hyphae	225:268	A red pigmented actinobacterium designated G2T, forming extremely branched vegetative hyphae, vesicles and mutilocular sporangia, was isolated from Casuarina equisetifolia nodules.
30010524	2	63	theme	red	179:181	arg1	vesicles					271:278	vesicles	271:278	vesicles	271:278	A red pigmented actinobacterium designated G2T, forming extremely branched vegetative hyphae, vesicles and mutilocular sporangia, was isolated from Casuarina equisetifolia nodules.
30010524	2	63	theme	red	179:181	arg1	sporangia					296:304	mutilocular sporangia	284:304	mutilocular sporangia	284:304	A red pigmented actinobacterium designated G2T, forming extremely branched vegetative hyphae, vesicles and mutilocular sporangia, was isolated from Casuarina equisetifolia nodules.
30010524	2	63	theme	red	179:181	arg1	actinobacterium					193:207	A red pigmented actinobacterium	177:207	A red pigmented actinobacterium designated G2T	177:222	A red pigmented actinobacterium designated G2T, forming extremely branched vegetative hyphae, vesicles and mutilocular sporangia, was isolated from Casuarina equisetifolia nodules.
30010524	2	64	theme	vegetative	252:261	arg1	actinobacterium					193:207	A red pigmented actinobacterium	177:207	A red pigmented actinobacterium designated G2T	177:222	A red pigmented actinobacterium designated G2T, forming extremely branched vegetative hyphae, vesicles and mutilocular sporangia, was isolated from Casuarina equisetifolia nodules.
30010524	2	64	theme	vegetative	252:261	arg1	hyphae					263:268	forming extremely branched vegetative hyphae	225:268	forming extremely branched vegetative hyphae	225:268	A red pigmented actinobacterium designated G2T, forming extremely branched vegetative hyphae, vesicles and mutilocular sporangia, was isolated from Casuarina equisetifolia nodules.
30010524	11	65	theme	type	1481:1484	arg1	strain					1486:1491	the type strain	1477:1491	the type strain of a novel species Frankia irregularis sp	1477:1533	On the basis of these results, strain G2T (=DSM 45899T=CECT 9038T) is proposed to represent the type strain of a novel species Frankia irregularis sp.
30010524	3	66	theme	Rhamnales	462:470	arg1	members					438:444	nodulated members	428:444	its original host plant but effectively nodulated members of actinorhizal Rhamnales	388:470	The strain failed to nodulate its original host plant but effectively nodulated members of actinorhizal Rhamnales.
30010524	2	67	theme	branched	243:250	arg1	actinobacterium					193:207	A red pigmented actinobacterium	177:207	A red pigmented actinobacterium designated G2T	177:222	A red pigmented actinobacterium designated G2T, forming extremely branched vegetative hyphae, vesicles and mutilocular sporangia, was isolated from Casuarina equisetifolia nodules.
30010524	2	67	theme	branched	243:250	arg1	hyphae					263:268	forming extremely branched vegetative hyphae	225:268	forming extremely branched vegetative hyphae	225:268	A red pigmented actinobacterium designated G2T, forming extremely branched vegetative hyphae, vesicles and mutilocular sporangia, was isolated from Casuarina equisetifolia nodules.
30010524	10	68	theme	hybridisation	1234:1246	arg1	values					1255:1260	Digital DNA -DNA hybridisation (dDDH) values	1217:1260	Digital DNA -DNA hybridisation (dDDH) values between G2T and its nearest phylogenetic neighbours Frankia	1217:1320	Digital DNA -DNA hybridisation (dDDH) values between G2T and its nearest phylogenetic neighbours Frankia elaeagniand Frankia discariaewere below the threshold of 70 %.
30010524	3	69	theme	original	392:399	arg1	plant					406:410	its original host plant	388:410	its original host plant but effectively nodulated members of actinorhizal Rhamnales	388:470	The strain failed to nodulate its original host plant but effectively nodulated members of actinorhizal Rhamnales.
29974158	5	0	theme	35-65 °C	738:745	arg1	salinities					687:696	salinities	687:696	salinities of 0-4% (optimum 2-3%)	687:719	Growth of strain BM62T was observed at salinities of 0-4% (optimum 2-3%), temperatures of 35-65 °C (optimum 50 °C) and pH values of 5-9 (optimum pH 7).
29974158	5	0	theme	35-65 °C	738:745	arg1	values					770:775	pH values	767:775	pH values of 5-9 (optimum pH 7)	767:797	Growth of strain BM62T was observed at salinities of 0-4% (optimum 2-3%), temperatures of 35-65 °C (optimum 50 °C) and pH values of 5-9 (optimum pH 7).
29974158	5	0	theme	35-65 °C	738:745	arg1	temperatures					722:733	temperatures	722:733	temperatures of 35-65 °C (optimum 50 °C)	722:761	Growth of strain BM62T was observed at salinities of 0-4% (optimum 2-3%), temperatures of 35-65 °C (optimum 50 °C) and pH values of 5-9 (optimum pH 7).
29974158	9	1	theme	36.5 mol	1111:1118	arg1	%					1119:1119	36.5 mol%	1111:1119	36.5 mol%	1111:1119	The G + C content of its genomic DNA was 36.5 mol%.
29974158	9	1	theme	36.5 mol	1111:1118	arg1	content					1080:1086	The G + C content	1070:1086	The G + C content of its genomic DNA	1070:1105	The G + C content of its genomic DNA was 36.5 mol%.
29974158	3	2	theme	16S	333:335	arg1	gene					342:345	the 16S rRNA gene	329:345	the 16S rRNA gene	329:345	Phylogenetic analysis based on the 16S rRNA gene indicated that strain BM62T belongs to the family Bacillaceae within the class Bacilli and was most closely related to Alteribacillus iranensis X5BT (only 94.6% sequence similarity).
29974158	12	3	theme	type	1380:1383	arg1	strain					1385:1390	The type strain	1376:1390	The type strain of the species	1376:1405	The type strain of the species Aliibacillus thermotolerans sp.
29974158	5	4	theme	strain	658:663	arg1	BM62T					665:669	strain BM62T	658:669	strain BM62T	658:669	Growth of strain BM62T was observed at salinities of 0-4% (optimum 2-3%), temperatures of 35-65 °C (optimum 50 °C) and pH values of 5-9 (optimum pH 7).
29974158	7	5	theme	peptidoglycan	890:902	arg1	A1γ					912:914	A1γ	912:914	A1γ	912:914	The peptidoglycan type is A1γ, and meso-diaminopimelic acid was the diagnostic diamino acid.
29974158	7	5	theme	peptidoglycan	890:902	arg1	type					904:907	The peptidoglycan type	886:907	The peptidoglycan type	886:907	The peptidoglycan type is A1γ, and meso-diaminopimelic acid was the diagnostic diamino acid.
29974158	10	6	theme	taxonomy	1148:1155	arg1	study					1157:1161	this polyphasic taxonomy study	1132:1161	this polyphasic taxonomy study	1132:1161	Data from this polyphasic taxonomy study suggested that strain BM62T should be classified as the type strain of the type species of a new genus within the family Bacillaceae for which the name Aliibacillus thermotolerans gen. nov., sp.
29974158	3	7	theme	strain	362:367	arg1	BM62T					369:373	strain BM62T	362:373	strain BM62T	362:373	Phylogenetic analysis based on the 16S rRNA gene indicated that strain BM62T belongs to the family Bacillaceae within the class Bacilli and was most closely related to Alteribacillus iranensis X5BT (only 94.6% sequence similarity).
29974158	5	8	theme	BM62T	665:669	arg1	Growth					648:653	Growth	648:653	Growth of strain BM62T	648:669	Growth of strain BM62T was observed at salinities of 0-4% (optimum 2-3%), temperatures of 35-65 °C (optimum 50 °C) and pH values of 5-9 (optimum pH 7).
29974158	10	9	theme	name	1310:1313	arg1	Aliibacillus					1315:1326	the name Aliibacillus	1306:1326	the name Aliibacillus	1306:1326	Data from this polyphasic taxonomy study suggested that strain BM62T should be classified as the type strain of the type species of a new genus within the family Bacillaceae for which the name Aliibacillus thermotolerans gen. nov., sp.
29974158	2	10	attach	isolated	232:239	arg1	compost					246:252	compost	246:252	compost	246:252	A novel moderately thermophilic and heterotrophic ammonia-oxidizing bacterium, designated strain BM62T, was isolated from compost in the thermophilic stage in Harbin, China.
29974158	2	10	attach	isolated	232:239	arg2	bacterium					192:200	A novel moderately thermophilic and heterotrophic ammonia-oxidizing bacterium	124:200	A novel moderately thermophilic and heterotrophic ammonia-oxidizing bacterium	124:200	A novel moderately thermophilic and heterotrophic ammonia-oxidizing bacterium, designated strain BM62T, was isolated from compost in the thermophilic stage in Harbin, China.
29974158	14	11	theme	DPD	1502:1504	arg1	Number					1512:1517	The respective DPD Taxon Number	1487:1517	The respective DPD Taxon Number	1487:1517	The respective DPD Taxon Number is GA00057.
29974158	14	11	theme	DPD	1502:1504	arg1	GA00057					1522:1528	GA00057	1522:1528	GA00057	1522:1528	The respective DPD Taxon Number is GA00057.
29974158	2	12	theme	heterotrophic	160:172	arg1	bacterium					192:200	A novel moderately thermophilic and heterotrophic ammonia-oxidizing bacterium	124:200	A novel moderately thermophilic and heterotrophic ammonia-oxidizing bacterium	124:200	A novel moderately thermophilic and heterotrophic ammonia-oxidizing bacterium, designated strain BM62T, was isolated from compost in the thermophilic stage in Harbin, China.
29974158	10	13	theme	genus	1260:1264	arg1	species					1243:1249	the type species	1234:1249	the type species of a new genus within the family Bacillaceae for which the name Aliibacillus thermotolerans gen. nov., sp	1234:1355	Data from this polyphasic taxonomy study suggested that strain BM62T should be classified as the type strain of the type species of a new genus within the family Bacillaceae for which the name Aliibacillus thermotolerans gen. nov., sp.
29974158	5	14	theme	optimum	785:791	arg1	pH					793:794	optimum pH 7	785:796	optimum pH 7	785:796	Growth of strain BM62T was observed at salinities of 0-4% (optimum 2-3%), temperatures of 35-65 °C (optimum 50 °C) and pH values of 5-9 (optimum pH 7).
29974158	5	14	theme	optimum	785:791	arg1	5-9					780:782	5-9	780:782	5-9 (optimum pH 7)	780:797	Growth of strain BM62T was observed at salinities of 0-4% (optimum 2-3%), temperatures of 35-65 °C (optimum 50 °C) and pH values of 5-9 (optimum pH 7).
29974158	3	15	theme	Alteribacillus	466:479	arg1	X5BT					491:494	Alteribacillus iranensis X5BT	466:494	Alteribacillus iranensis X5BT (only 94.6% sequence similarity)	466:527	Phylogenetic analysis based on the 16S rRNA gene indicated that strain BM62T belongs to the family Bacillaceae within the class Bacilli and was most closely related to Alteribacillus iranensis X5BT (only 94.6% sequence similarity).
29974158	3	15	theme	Alteribacillus	466:479	arg1	similarity					517:526	only 94.6% sequence similarity	497:526	only 94.6% sequence similarity	497:526	Phylogenetic analysis based on the 16S rRNA gene indicated that strain BM62T belongs to the family Bacillaceae within the class Bacilli and was most closely related to Alteribacillus iranensis X5BT (only 94.6% sequence similarity).
29974158	1	16	theme	thermophilic	51:62	arg1	bacterium					100:108	a thermophilic and heterotrophic ammonia-oxidizing bacterium	49:108	nov.: a thermophilic and heterotrophic ammonia-oxidizing bacterium from compost.	43:122	nov.: a thermophilic and heterotrophic ammonia-oxidizing bacterium from compost.
29974158	2	17	theme	thermophilic	143:154	arg1	bacterium					192:200	A novel moderately thermophilic and heterotrophic ammonia-oxidizing bacterium	124:200	A novel moderately thermophilic and heterotrophic ammonia-oxidizing bacterium	124:200	A novel moderately thermophilic and heterotrophic ammonia-oxidizing bacterium, designated strain BM62T, was isolated from compost in the thermophilic stage in Harbin, China.
29974158	10	18	theme	new	1256:1258	arg1	genus					1260:1264	a new genus	1254:1264	a new genus within the family Bacillaceae for which the name Aliibacillus thermotolerans gen. nov., sp	1254:1355	Data from this polyphasic taxonomy study suggested that strain BM62T should be classified as the type strain of the type species of a new genus within the family Bacillaceae for which the name Aliibacillus thermotolerans gen. nov., sp.
29974158	10	19	theme	type	1219:1222	arg1	strain					1224:1229	the type strain	1215:1229	the type strain of the type species of a new genus within the family Bacillaceae for which the name Aliibacillus thermotolerans gen. nov., sp	1215:1355	Data from this polyphasic taxonomy study suggested that strain BM62T should be classified as the type strain of the type species of a new genus within the family Bacillaceae for which the name Aliibacillus thermotolerans gen. nov., sp.
29974158	10	19	theme	type	1219:1222	arg1	BM62T					1185:1189	strain BM62T	1178:1189	strain BM62T	1178:1189	Data from this polyphasic taxonomy study suggested that strain BM62T should be classified as the type strain of the type species of a new genus within the family Bacillaceae for which the name Aliibacillus thermotolerans gen. nov., sp.
29974158	7	20	theme	diagnostic	954:963	arg1	acid					973:976	the diagnostic diamino acid	950:976	the diagnostic diamino acid	950:976	The peptidoglycan type is A1γ, and meso-diaminopimelic acid was the diagnostic diamino acid.
29974158	7	20	theme	diagnostic	954:963	arg1	acid					941:944	meso-diaminopimelic acid	921:944	meso-diaminopimelic acid	921:944	The peptidoglycan type is A1γ, and meso-diaminopimelic acid was the diagnostic diamino acid.
29974158	5	21	theme	pH	767:768	arg1	values					770:775	pH values	767:775	pH values of 5-9 (optimum pH 7)	767:797	Growth of strain BM62T was observed at salinities of 0-4% (optimum 2-3%), temperatures of 35-65 °C (optimum 50 °C) and pH values of 5-9 (optimum pH 7).
29974158	6	22	theme	cellular	810:817	arg1	acid					825:828	The major cellular fatty acid	800:828	The major cellular fatty acid	800:828	The major cellular fatty acid was iso-C16:0, and the predominant ubiquinone was MK-7.
29974158	6	22	theme	cellular	810:817	arg1	iso-C16:0					834:842	iso-C16:0	834:842	iso-C16:0	834:842	The major cellular fatty acid was iso-C16:0, and the predominant ubiquinone was MK-7.
29974158	1	23	theme	heterotrophic	68:80	arg1	bacterium					100:108	a thermophilic and heterotrophic ammonia-oxidizing bacterium	49:108	nov.: a thermophilic and heterotrophic ammonia-oxidizing bacterium from compost.	43:122	nov.: a thermophilic and heterotrophic ammonia-oxidizing bacterium from compost.
29974158	14	24	theme	respective	1491:1500	arg1	Number					1512:1517	The respective DPD Taxon Number	1487:1517	The respective DPD Taxon Number	1487:1517	The respective DPD Taxon Number is GA00057.
29974158	14	24	theme	respective	1491:1500	arg1	GA00057					1522:1528	GA00057	1522:1528	GA00057	1522:1528	The respective DPD Taxon Number is GA00057.
29974158	3	25	theme	family	390:395	arg1	Bacillaceae					397:407	the family Bacillaceae	386:407	the family Bacillaceae within the class Bacilli	386:432	Phylogenetic analysis based on the 16S rRNA gene indicated that strain BM62T belongs to the family Bacillaceae within the class Bacilli and was most closely related to Alteribacillus iranensis X5BT (only 94.6% sequence similarity).
29974158	4	26	theme	BM62T	546:550	arg1	Cells					530:534	Cells	530:534	Cells of strain BM62T	530:550	Cells of strain BM62T were Gram-positive, rod-shaped, motile by periflagella, catalase-positive and oxidase-negative.
29974158	8	27	theme	polar	989:993	arg1	diphosphatidylglycerol					1007:1028	diphosphatidylglycerol	1007:1028	diphosphatidylglycerol	1007:1028	The major polar lipids were diphosphatidylglycerol, phospholipid and phosphatidylglycerol.
29974158	8	27	theme	polar	989:993	arg1	lipids					995:1000	The major polar lipids	979:1000	The major polar lipids	979:1000	The major polar lipids were diphosphatidylglycerol, phospholipid and phosphatidylglycerol.
29974158	1	28	from	compost	115:121	arg1	bacterium					100:108	a thermophilic and heterotrophic ammonia-oxidizing bacterium	49:108	nov.: a thermophilic and heterotrophic ammonia-oxidizing bacterium from compost.	43:122	nov.: a thermophilic and heterotrophic ammonia-oxidizing bacterium from compost.
29974158	1	29	theme	ammonia-oxidizing	82:98	arg1	bacterium					100:108	a thermophilic and heterotrophic ammonia-oxidizing bacterium	49:108	nov.: a thermophilic and heterotrophic ammonia-oxidizing bacterium from compost.	43:122	nov.: a thermophilic and heterotrophic ammonia-oxidizing bacterium from compost.
29974158	12	30	theme	Aliibacillus	1407:1418	arg1	sp					1435:1436	Aliibacillus thermotolerans sp	1407:1436	Aliibacillus thermotolerans sp	1407:1436	The type strain of the species Aliibacillus thermotolerans sp.
29974158	12	31	dep	sp	1435:1436	arg1	strain					1385:1390	The type strain	1376:1390	The type strain of the species	1376:1405	The type strain of the species Aliibacillus thermotolerans sp.
29974158	4	32	theme	strain	539:544	arg1	BM62T					546:550	strain BM62T	539:550	strain BM62T	539:550	Cells of strain BM62T were Gram-positive, rod-shaped, motile by periflagella, catalase-positive and oxidase-negative.
29974158	8	33	theme	major	983:987	arg1	diphosphatidylglycerol					1007:1028	diphosphatidylglycerol	1007:1028	diphosphatidylglycerol	1007:1028	The major polar lipids were diphosphatidylglycerol, phospholipid and phosphatidylglycerol.
29974158	8	33	theme	major	983:987	arg1	lipids					995:1000	The major polar lipids	979:1000	The major polar lipids	979:1000	The major polar lipids were diphosphatidylglycerol, phospholipid and phosphatidylglycerol.
29974158	5	34	located	observed	675:682	arg1	salinities					687:696	salinities	687:696	salinities of 0-4% (optimum 2-3%)	687:719	Growth of strain BM62T was observed at salinities of 0-4% (optimum 2-3%), temperatures of 35-65 °C (optimum 50 °C) and pH values of 5-9 (optimum pH 7).
29974158	5	34	located	observed	675:682	arg1	values					770:775	pH values	767:775	pH values of 5-9 (optimum pH 7)	767:797	Growth of strain BM62T was observed at salinities of 0-4% (optimum 2-3%), temperatures of 35-65 °C (optimum 50 °C) and pH values of 5-9 (optimum pH 7).
29974158	5	34	located	observed	675:682	arg2	Growth					648:653	Growth	648:653	Growth of strain BM62T	648:669	Growth of strain BM62T was observed at salinities of 0-4% (optimum 2-3%), temperatures of 35-65 °C (optimum 50 °C) and pH values of 5-9 (optimum pH 7).
29974158	5	34	located	observed	675:682	arg1	temperatures					722:733	temperatures	722:733	temperatures of 35-65 °C (optimum 50 °C)	722:761	Growth of strain BM62T was observed at salinities of 0-4% (optimum 2-3%), temperatures of 35-65 °C (optimum 50 °C) and pH values of 5-9 (optimum pH 7).
29974158	0	35	theme	gen.	28:31	arg1	sp					39:40	sp	39:40	sp	39:40	Aliibacillus thermotolerans gen. nov., sp.
29974158	0	35	theme	gen.	28:31	arg1	nov.					33:36	gen. nov.	28:36	gen. nov.	28:36	Aliibacillus thermotolerans gen. nov., sp.
29974158	5	36	theme	%	704:704	arg1	salinities					687:696	salinities	687:696	salinities of 0-4% (optimum 2-3%)	687:719	Growth of strain BM62T was observed at salinities of 0-4% (optimum 2-3%), temperatures of 35-65 °C (optimum 50 °C) and pH values of 5-9 (optimum pH 7).
29974158	5	36	theme	%	704:704	arg1	values					770:775	pH values	767:775	pH values of 5-9 (optimum pH 7)	767:797	Growth of strain BM62T was observed at salinities of 0-4% (optimum 2-3%), temperatures of 35-65 °C (optimum 50 °C) and pH values of 5-9 (optimum pH 7).
29974158	5	36	theme	%	704:704	arg1	temperatures					722:733	temperatures	722:733	temperatures of 35-65 °C (optimum 50 °C)	722:761	Growth of strain BM62T was observed at salinities of 0-4% (optimum 2-3%), temperatures of 35-65 °C (optimum 50 °C) and pH values of 5-9 (optimum pH 7).
29974158	6	37	theme	major	804:808	arg1	acid					825:828	The major cellular fatty acid	800:828	The major cellular fatty acid	800:828	The major cellular fatty acid was iso-C16:0, and the predominant ubiquinone was MK-7.
29974158	6	37	theme	major	804:808	arg1	iso-C16:0					834:842	iso-C16:0	834:842	iso-C16:0	834:842	The major cellular fatty acid was iso-C16:0, and the predominant ubiquinone was MK-7.
29974158	3	38	dep	similarity	517:526	arg1	%					506:506	%	506:506	%	506:506	Phylogenetic analysis based on the 16S rRNA gene indicated that strain BM62T belongs to the family Bacillaceae within the class Bacilli and was most closely related to Alteribacillus iranensis X5BT (only 94.6% sequence similarity).
29974158	10	39	theme	type	1238:1241	arg1	species					1243:1249	the type species	1234:1249	the type species of a new genus within the family Bacillaceae for which the name Aliibacillus thermotolerans gen. nov., sp	1234:1355	Data from this polyphasic taxonomy study suggested that strain BM62T should be classified as the type strain of the type species of a new genus within the family Bacillaceae for which the name Aliibacillus thermotolerans gen. nov., sp.
29974158	1	40	dep	nov.	43:46	arg1	bacterium					100:108	a thermophilic and heterotrophic ammonia-oxidizing bacterium	49:108	nov.: a thermophilic and heterotrophic ammonia-oxidizing bacterium from compost.	43:122	nov.: a thermophilic and heterotrophic ammonia-oxidizing bacterium from compost.
29974158	5	41	theme	optimum	748:754	arg1	35-65 °C					738:745	35-65 °C	738:745	35-65 °C (optimum 50 °C)	738:761	Growth of strain BM62T was observed at salinities of 0-4% (optimum 2-3%), temperatures of 35-65 °C (optimum 50 °C) and pH values of 5-9 (optimum pH 7).
29974158	5	41	theme	optimum	748:754	arg1	50 °C					756:760	optimum 50 °C	748:760	optimum 50 °C	748:760	Growth of strain BM62T was observed at salinities of 0-4% (optimum 2-3%), temperatures of 35-65 °C (optimum 50 °C) and pH values of 5-9 (optimum pH 7).
29974158	12	42	theme	thermotolerans	1420:1433	arg1	sp					1435:1436	Aliibacillus thermotolerans sp	1407:1436	Aliibacillus thermotolerans sp	1407:1436	The type strain of the species Aliibacillus thermotolerans sp.
29974158	10	43	from	study	1157:1161	arg1	Data					1122:1125	Data	1122:1125	Data from this polyphasic taxonomy study	1122:1161	Data from this polyphasic taxonomy study suggested that strain BM62T should be classified as the type strain of the type species of a new genus within the family Bacillaceae for which the name Aliibacillus thermotolerans gen. nov., sp.
29974158	13	44	theme	= DSM	1454:1458	arg1	BM62T					1447:1451	BM62T	1447:1451	BM62T (= DSM 101851T = CGMCC 1.15790T)	1447:1484	nov. is BM62T (= DSM 101851T = CGMCC 1.15790T).
29974158	13	44	theme	= DSM	1454:1458	arg1	1.15790T					1476:1483	= DSM 101851T = CGMCC 1.15790T	1454:1483	= DSM 101851T = CGMCC 1.15790T	1454:1483	nov. is BM62T (= DSM 101851T = CGMCC 1.15790T).
29974158	2	45	from	stage	274:278	arg1	China					291:295	China	291:295	China	291:295	A novel moderately thermophilic and heterotrophic ammonia-oxidizing bacterium, designated strain BM62T, was isolated from compost in the thermophilic stage in Harbin, China.
29974158	2	45	from	stage	274:278	arg1	Harbin					283:288	Harbin	283:288	Harbin	283:288	A novel moderately thermophilic and heterotrophic ammonia-oxidizing bacterium, designated strain BM62T, was isolated from compost in the thermophilic stage in Harbin, China.
29974158	3	46	theme	class	420:424	arg1	Bacilli					426:432	the class Bacilli	416:432	the class Bacilli	416:432	Phylogenetic analysis based on the 16S rRNA gene indicated that strain BM62T belongs to the family Bacillaceae within the class Bacilli and was most closely related to Alteribacillus iranensis X5BT (only 94.6% sequence similarity).
29974158	5	47	theme	optimum	707:713	arg1	%					704:704	0-4%	701:704	0-4% (optimum 2-3%)	701:719	Growth of strain BM62T was observed at salinities of 0-4% (optimum 2-3%), temperatures of 35-65 °C (optimum 50 °C) and pH values of 5-9 (optimum pH 7).
29974158	5	47	theme	optimum	707:713	arg1	%					718:718	optimum 2-3%	707:718	optimum 2-3%	707:718	Growth of strain BM62T was observed at salinities of 0-4% (optimum 2-3%), temperatures of 35-65 °C (optimum 50 °C) and pH values of 5-9 (optimum pH 7).
29974158	13	48	theme	101851T = CGMCC	1460:1474	arg1	BM62T					1447:1451	BM62T	1447:1451	BM62T (= DSM 101851T = CGMCC 1.15790T)	1447:1484	nov. is BM62T (= DSM 101851T = CGMCC 1.15790T).
29974158	13	48	theme	101851T = CGMCC	1460:1474	arg1	1.15790T					1476:1483	= DSM 101851T = CGMCC 1.15790T	1454:1483	= DSM 101851T = CGMCC 1.15790T	1454:1483	nov. is BM62T (= DSM 101851T = CGMCC 1.15790T).
29974158	9	49	theme	G + C	1074:1078	arg1	%					1119:1119	36.5 mol%	1111:1119	36.5 mol%	1111:1119	The G + C content of its genomic DNA was 36.5 mol%.
29974158	9	49	theme	G + C	1074:1078	arg1	content					1080:1086	The G + C content	1070:1086	The G + C content of its genomic DNA	1070:1105	The G + C content of its genomic DNA was 36.5 mol%.
29974158	5	50	theme	2-3	715:717	arg1	%					704:704	0-4%	701:704	0-4% (optimum 2-3%)	701:719	Growth of strain BM62T was observed at salinities of 0-4% (optimum 2-3%), temperatures of 35-65 °C (optimum 50 °C) and pH values of 5-9 (optimum pH 7).
29974158	5	50	theme	2-3	715:717	arg1	%					718:718	optimum 2-3%	707:718	optimum 2-3%	707:718	Growth of strain BM62T was observed at salinities of 0-4% (optimum 2-3%), temperatures of 35-65 °C (optimum 50 °C) and pH values of 5-9 (optimum pH 7).
29974158	2	51	theme	ammonia-oxidizing	174:190	arg1	bacterium					192:200	A novel moderately thermophilic and heterotrophic ammonia-oxidizing bacterium	124:200	A novel moderately thermophilic and heterotrophic ammonia-oxidizing bacterium	124:200	A novel moderately thermophilic and heterotrophic ammonia-oxidizing bacterium, designated strain BM62T, was isolated from compost in the thermophilic stage in Harbin, China.
29974158	6	52	theme	fatty	819:823	arg1	acid					825:828	The major cellular fatty acid	800:828	The major cellular fatty acid	800:828	The major cellular fatty acid was iso-C16:0, and the predominant ubiquinone was MK-7.
29974158	6	52	theme	fatty	819:823	arg1	iso-C16:0					834:842	iso-C16:0	834:842	iso-C16:0	834:842	The major cellular fatty acid was iso-C16:0, and the predominant ubiquinone was MK-7.
29974158	10	53	theme	gen.	1343:1346	arg1	sp					1354:1355	sp	1354:1355	sp	1354:1355	Data from this polyphasic taxonomy study suggested that strain BM62T should be classified as the type strain of the type species of a new genus within the family Bacillaceae for which the name Aliibacillus thermotolerans gen. nov., sp.
29974158	10	53	theme	gen.	1343:1346	arg1	nov.					1348:1351	gen. nov.	1343:1351	gen. nov.	1343:1351	Data from this polyphasic taxonomy study suggested that strain BM62T should be classified as the type strain of the type species of a new genus within the family Bacillaceae for which the name Aliibacillus thermotolerans gen. nov., sp.
29974158	7	54	theme	meso-diaminopimelic	921:939	arg1	acid					973:976	the diagnostic diamino acid	950:976	the diagnostic diamino acid	950:976	The peptidoglycan type is A1γ, and meso-diaminopimelic acid was the diagnostic diamino acid.
29974158	7	54	theme	meso-diaminopimelic	921:939	arg1	acid					941:944	meso-diaminopimelic acid	921:944	meso-diaminopimelic acid	921:944	The peptidoglycan type is A1γ, and meso-diaminopimelic acid was the diagnostic diamino acid.
29974158	3	55	dep	%	506:506	arg1	94.6					502:505	94.6	502:505	94.6	502:505	Phylogenetic analysis based on the 16S rRNA gene indicated that strain BM62T belongs to the family Bacillaceae within the class Bacilli and was most closely related to Alteribacillus iranensis X5BT (only 94.6% sequence similarity).
29974158	6	56	theme	predominant	853:863	arg1	MK-7					880:883	MK-7	880:883	MK-7	880:883	The major cellular fatty acid was iso-C16:0, and the predominant ubiquinone was MK-7.
29974158	6	56	theme	predominant	853:863	arg1	ubiquinone					865:874	the predominant ubiquinone	849:874	the predominant ubiquinone	849:874	The major cellular fatty acid was iso-C16:0, and the predominant ubiquinone was MK-7.
29974158	3	57	theme	Phylogenetic	298:309	arg1	analysis					311:318	Phylogenetic analysis	298:318	Phylogenetic analysis based on the 16S rRNA gene	298:345	Phylogenetic analysis based on the 16S rRNA gene indicated that strain BM62T belongs to the family Bacillaceae within the class Bacilli and was most closely related to Alteribacillus iranensis X5BT (only 94.6% sequence similarity).
29974158	14	58	theme	Taxon	1506:1510	arg1	Number					1512:1517	The respective DPD Taxon Number	1487:1517	The respective DPD Taxon Number	1487:1517	The respective DPD Taxon Number is GA00057.
29974158	14	58	theme	Taxon	1506:1510	arg1	GA00057					1522:1528	GA00057	1522:1528	GA00057	1522:1528	The respective DPD Taxon Number is GA00057.
29974158	3	59	theme	iranensis	481:489	arg1	X5BT					491:494	Alteribacillus iranensis X5BT	466:494	Alteribacillus iranensis X5BT (only 94.6% sequence similarity)	466:527	Phylogenetic analysis based on the 16S rRNA gene indicated that strain BM62T belongs to the family Bacillaceae within the class Bacilli and was most closely related to Alteribacillus iranensis X5BT (only 94.6% sequence similarity).
29974158	3	59	theme	iranensis	481:489	arg1	similarity					517:526	only 94.6% sequence similarity	497:526	only 94.6% sequence similarity	497:526	Phylogenetic analysis based on the 16S rRNA gene indicated that strain BM62T belongs to the family Bacillaceae within the class Bacilli and was most closely related to Alteribacillus iranensis X5BT (only 94.6% sequence similarity).
29974158	10	60	theme	strain	1178:1183	arg1	strain					1224:1229	the type strain	1215:1229	the type strain of the type species of a new genus within the family Bacillaceae for which the name Aliibacillus thermotolerans gen. nov., sp	1215:1355	Data from this polyphasic taxonomy study suggested that strain BM62T should be classified as the type strain of the type species of a new genus within the family Bacillaceae for which the name Aliibacillus thermotolerans gen. nov., sp.
29974158	10	60	theme	strain	1178:1183	arg1	BM62T					1185:1189	strain BM62T	1178:1189	strain BM62T	1178:1189	Data from this polyphasic taxonomy study suggested that strain BM62T should be classified as the type strain of the type species of a new genus within the family Bacillaceae for which the name Aliibacillus thermotolerans gen. nov., sp.
29974158	7	61	theme	diamino	965:971	arg1	acid					973:976	the diagnostic diamino acid	950:976	the diagnostic diamino acid	950:976	The peptidoglycan type is A1γ, and meso-diaminopimelic acid was the diagnostic diamino acid.
29974158	7	61	theme	diamino	965:971	arg1	acid					941:944	meso-diaminopimelic acid	921:944	meso-diaminopimelic acid	921:944	The peptidoglycan type is A1γ, and meso-diaminopimelic acid was the diagnostic diamino acid.
29974158	12	62	theme	species	1399:1405	arg1	strain					1385:1390	The type strain	1376:1390	The type strain of the species	1376:1405	The type strain of the species Aliibacillus thermotolerans sp.
29974158	9	63	theme	genomic	1095:1101	arg1	DNA					1103:1105	its genomic DNA	1091:1105	its genomic DNA	1091:1105	The G + C content of its genomic DNA was 36.5 mol%.
29974158	10	64	theme	species	1243:1249	arg1	strain					1224:1229	the type strain	1215:1229	the type strain of the type species of a new genus within the family Bacillaceae for which the name Aliibacillus thermotolerans gen. nov., sp	1215:1355	Data from this polyphasic taxonomy study suggested that strain BM62T should be classified as the type strain of the type species of a new genus within the family Bacillaceae for which the name Aliibacillus thermotolerans gen. nov., sp.
29974158	10	64	theme	species	1243:1249	arg1	BM62T					1185:1189	strain BM62T	1178:1189	strain BM62T	1178:1189	Data from this polyphasic taxonomy study suggested that strain BM62T should be classified as the type strain of the type species of a new genus within the family Bacillaceae for which the name Aliibacillus thermotolerans gen. nov., sp.
29974158	3	65	theme	rRNA	337:340	arg1	gene					342:345	the 16S rRNA gene	329:345	the 16S rRNA gene	329:345	Phylogenetic analysis based on the 16S rRNA gene indicated that strain BM62T belongs to the family Bacillaceae within the class Bacilli and was most closely related to Alteribacillus iranensis X5BT (only 94.6% sequence similarity).
29974158	2	66	theme	strain	214:219	arg1	BM62T					221:225	strain BM62T	214:225	strain BM62T	214:225	A novel moderately thermophilic and heterotrophic ammonia-oxidizing bacterium, designated strain BM62T, was isolated from compost in the thermophilic stage in Harbin, China.
29974158	2	67	theme	thermophilic	261:272	arg1	stage					274:278	the thermophilic stage	257:278	the thermophilic stage in Harbin, China	257:295	A novel moderately thermophilic and heterotrophic ammonia-oxidizing bacterium, designated strain BM62T, was isolated from compost in the thermophilic stage in Harbin, China.
29974158	9	68	theme	DNA	1103:1105	arg1	%					1119:1119	36.5 mol%	1111:1119	36.5 mol%	1111:1119	The G + C content of its genomic DNA was 36.5 mol%.
29974158	9	68	theme	DNA	1103:1105	arg1	content					1080:1086	The G + C content	1070:1086	The G + C content of its genomic DNA	1070:1105	The G + C content of its genomic DNA was 36.5 mol%.
29974158	3	69	theme	sequence	508:515	arg1	X5BT					491:494	Alteribacillus iranensis X5BT	466:494	Alteribacillus iranensis X5BT (only 94.6% sequence similarity)	466:527	Phylogenetic analysis based on the 16S rRNA gene indicated that strain BM62T belongs to the family Bacillaceae within the class Bacilli and was most closely related to Alteribacillus iranensis X5BT (only 94.6% sequence similarity).
29974158	3	69	theme	sequence	508:515	arg1	similarity					517:526	only 94.6% sequence similarity	497:526	only 94.6% sequence similarity	497:526	Phylogenetic analysis based on the 16S rRNA gene indicated that strain BM62T belongs to the family Bacillaceae within the class Bacilli and was most closely related to Alteribacillus iranensis X5BT (only 94.6% sequence similarity).
29974158	10	70	theme	polyphasic	1137:1146	arg1	study					1157:1161	this polyphasic taxonomy study	1132:1161	this polyphasic taxonomy study	1132:1161	Data from this polyphasic taxonomy study suggested that strain BM62T should be classified as the type strain of the type species of a new genus within the family Bacillaceae for which the name Aliibacillus thermotolerans gen. nov., sp.
29974158	2	71	theme	novel	126:130	arg1	bacterium					192:200	A novel moderately thermophilic and heterotrophic ammonia-oxidizing bacterium	124:200	A novel moderately thermophilic and heterotrophic ammonia-oxidizing bacterium	124:200	A novel moderately thermophilic and heterotrophic ammonia-oxidizing bacterium, designated strain BM62T, was isolated from compost in the thermophilic stage in Harbin, China.
29974158	5	72	theme	5-9	780:782	arg1	salinities					687:696	salinities	687:696	salinities of 0-4% (optimum 2-3%)	687:719	Growth of strain BM62T was observed at salinities of 0-4% (optimum 2-3%), temperatures of 35-65 °C (optimum 50 °C) and pH values of 5-9 (optimum pH 7).
29974158	5	72	theme	5-9	780:782	arg1	values					770:775	pH values	767:775	pH values of 5-9 (optimum pH 7)	767:797	Growth of strain BM62T was observed at salinities of 0-4% (optimum 2-3%), temperatures of 35-65 °C (optimum 50 °C) and pH values of 5-9 (optimum pH 7).
29974158	5	72	theme	5-9	780:782	arg1	temperatures					722:733	temperatures	722:733	temperatures of 35-65 °C (optimum 50 °C)	722:761	Growth of strain BM62T was observed at salinities of 0-4% (optimum 2-3%), temperatures of 35-65 °C (optimum 50 °C) and pH values of 5-9 (optimum pH 7).
29974158	10	73	theme	family	1277:1282	arg1	Bacillaceae					1284:1294	the family Bacillaceae	1273:1294	the family Bacillaceae for which the name Aliibacillus thermotolerans gen. nov., sp	1273:1355	Data from this polyphasic taxonomy study suggested that strain BM62T should be classified as the type strain of the type species of a new genus within the family Bacillaceae for which the name Aliibacillus thermotolerans gen. nov., sp.
29583112	6	0	theme	16S	893:895	arg1	similarity					916:925	16S rRNA gene sequence similarity	893:925	16S rRNA gene sequence similarity	893:925	Based on 16S rRNA gene sequence similarity, the strain was related to Enterorhabdus musicola DSM 19490T (92.9 % similarity), Enterorhabdus caecimuris DSM 21839T (92.7 % similarity), Adlercreutzia equolifaciens DSM 19450T (92.5 % similarity), Asaccharobacter celatus DSM 18785T (92.5 % similarity) and Parvibacter caecicola DSM 22242T (91.2 % similarity).
29583112	6	1	theme	%	1051:1051	arg1	similarity					1053:1062	92.7 % similarity	1046:1062	92.7 % similarity	1046:1062	Based on 16S rRNA gene sequence similarity, the strain was related to Enterorhabdus musicola DSM 19490T (92.9 % similarity), Enterorhabdus caecimuris DSM 21839T (92.7 % similarity), Adlercreutzia equolifaciens DSM 19450T (92.5 % similarity), Asaccharobacter celatus DSM 18785T (92.5 % similarity) and Parvibacter caecicola DSM 22242T (91.2 % similarity).
29583112	6	1	theme	%	1051:1051	arg1	21839T					1038:1043	Enterorhabdus caecimuris DSM 21839T	1009:1043	Enterorhabdus caecimuris DSM 21839T (92.7 % similarity)	1009:1063	Based on 16S rRNA gene sequence similarity, the strain was related to Enterorhabdus musicola DSM 19490T (92.9 % similarity), Enterorhabdus caecimuris DSM 21839T (92.7 % similarity), Adlercreutzia equolifaciens DSM 19450T (92.5 % similarity), Asaccharobacter celatus DSM 18785T (92.5 % similarity) and Parvibacter caecicola DSM 22242T (91.2 % similarity).
29583112	6	2	theme	91.2 	1219:1223	arg1	similarity					1226:1235	91.2 % similarity	1219:1235	91.2 % similarity	1219:1235	Based on 16S rRNA gene sequence similarity, the strain was related to Enterorhabdus musicola DSM 19490T (92.9 % similarity), Enterorhabdus caecimuris DSM 21839T (92.7 % similarity), Adlercreutzia equolifaciens DSM 19450T (92.5 % similarity), Asaccharobacter celatus DSM 18785T (92.5 % similarity) and Parvibacter caecicola DSM 22242T (91.2 % similarity).
29583112	6	2	theme	91.2 	1219:1223	arg1	22242T					1211:1216	Parvibacter caecicola DSM 22242T	1185:1216	Parvibacter caecicola DSM 22242T (91.2 % similarity)	1185:1236	Based on 16S rRNA gene sequence similarity, the strain was related to Enterorhabdus musicola DSM 19490T (92.9 % similarity), Enterorhabdus caecimuris DSM 21839T (92.7 % similarity), Adlercreutzia equolifaciens DSM 19450T (92.5 % similarity), Asaccharobacter celatus DSM 18785T (92.5 % similarity) and Parvibacter caecicola DSM 22242T (91.2 % similarity).
29583112	10	3	theme	several	1662:1668	arg1	phosphoglycolipids					1670:1687	several phosphoglycolipids	1662:1687	several phosphoglycolipids (PGL1-2)	1662:1696	Diphosphatidylglycerol and phosphatidylglycerol comprised the main polar lipid profile in addition to several phosphoglycolipids (PGL1-2), phospholipids (PL1-4), glycolipids (GL1-6) and lipids.
29583112	10	3	theme	several	1662:1668	arg1	PGL1-2					1690:1695	PGL1-2	1690:1695	PGL1-2	1690:1695	Diphosphatidylglycerol and phosphatidylglycerol comprised the main polar lipid profile in addition to several phosphoglycolipids (PGL1-2), phospholipids (PL1-4), glycolipids (GL1-6) and lipids.
29583112	6	4	theme	Asaccharobacter	1126:1140	arg1	18785T					1154:1159	Asaccharobacter celatus DSM 18785T	1126:1159	Asaccharobacter celatus DSM 18785T (92.5 % similarity)	1126:1179	Based on 16S rRNA gene sequence similarity, the strain was related to Enterorhabdus musicola DSM 19490T (92.9 % similarity), Enterorhabdus caecimuris DSM 21839T (92.7 % similarity), Adlercreutzia equolifaciens DSM 19450T (92.5 % similarity), Asaccharobacter celatus DSM 18785T (92.5 % similarity) and Parvibacter caecicola DSM 22242T (91.2 % similarity).
29583112	6	4	theme	Asaccharobacter	1126:1140	arg1	similarity					1169:1178	92.5 % similarity	1162:1178	92.5 % similarity	1162:1178	Based on 16S rRNA gene sequence similarity, the strain was related to Enterorhabdus musicola DSM 19490T (92.9 % similarity), Enterorhabdus caecimuris DSM 21839T (92.7 % similarity), Adlercreutzia equolifaciens DSM 19450T (92.5 % similarity), Asaccharobacter celatus DSM 18785T (92.5 % similarity) and Parvibacter caecicola DSM 22242T (91.2 % similarity).
29583112	4	5	theme	new	563:565	arg1	bacterium					567:575	a new bacterium	561:575	a new bacterium	561:575	We report here a new bacterium, capable of metabolizing EA into isourolithin A, isolated from healthy human faeces and characterized by determining phenotypic, biochemical and molecular methods.
29583112	8	6	theme	fatty	1314:1318	arg1	saturated					1331:1339	saturated	1331:1339	saturated	1331:1339	The whole-cell fatty acids were saturated (98.3 %), a very high percentage that differs from the nearest genera ranging from 62 to 73 %.
29583112	8	6	theme	fatty	1314:1318	arg1	%					1347:1347	98.3 %	1342:1347	98.3 %	1342:1347	The whole-cell fatty acids were saturated (98.3 %), a very high percentage that differs from the nearest genera ranging from 62 to 73 %.
29583112	8	6	theme	fatty	1314:1318	arg1	acids					1320:1324	The whole-cell fatty acids	1299:1324	The whole-cell fatty acids	1299:1324	The whole-cell fatty acids were saturated (98.3 %), a very high percentage that differs from the nearest genera ranging from 62 to 73 %.
29583112	8	6	theme	fatty	1314:1318	arg1	percentage					1363:1372	a very high percentage	1351:1372	a very high percentage that differs from the nearest genera ranging from 62 to 73 %	1351:1433	The whole-cell fatty acids were saturated (98.3 %), a very high percentage that differs from the nearest genera ranging from 62 to 73 %.
29583112	6	7	theme	caecicola	1197:1205	arg1	similarity					1226:1235	91.2 % similarity	1219:1235	91.2 % similarity	1219:1235	Based on 16S rRNA gene sequence similarity, the strain was related to Enterorhabdus musicola DSM 19490T (92.9 % similarity), Enterorhabdus caecimuris DSM 21839T (92.7 % similarity), Adlercreutzia equolifaciens DSM 19450T (92.5 % similarity), Asaccharobacter celatus DSM 18785T (92.5 % similarity) and Parvibacter caecicola DSM 22242T (91.2 % similarity).
29583112	6	7	theme	caecicola	1197:1205	arg1	22242T					1211:1216	Parvibacter caecicola DSM 22242T	1185:1216	Parvibacter caecicola DSM 22242T (91.2 % similarity)	1185:1236	Based on 16S rRNA gene sequence similarity, the strain was related to Enterorhabdus musicola DSM 19490T (92.9 % similarity), Enterorhabdus caecimuris DSM 21839T (92.7 % similarity), Adlercreutzia equolifaciens DSM 19450T (92.5 % similarity), Asaccharobacter celatus DSM 18785T (92.5 % similarity) and Parvibacter caecicola DSM 22242T (91.2 % similarity).
29583112	3	8	theme	isourolithin	517:528	arg1	A					530:530	isourolithin A	517:530	isourolithin A	517:530	Gordonibacter species produce some intermediary urolithins but the micro-organisms responsible for the transformation of EA into the final and more bioactive urolithins, such as urolithin A and isourolithin A, are unknown.
29583112	5	9	theme	family	834:839	arg1	genera					819:824	other genera	813:824	other genera of this family	813:839	Strain CEBAS 4A belongs to the Eggerthellaceae family and differed from other genera of this family, both phylogenetically and phenotypically.
29583112	6	10	theme	%	1111:1111	arg1	19450T					1098:1103	Adlercreutzia equolifaciens DSM 19450T	1066:1103	Adlercreutzia equolifaciens DSM 19450T (92.5 % similarity)	1066:1123	Based on 16S rRNA gene sequence similarity, the strain was related to Enterorhabdus musicola DSM 19490T (92.9 % similarity), Enterorhabdus caecimuris DSM 21839T (92.7 % similarity), Adlercreutzia equolifaciens DSM 19450T (92.5 % similarity), Asaccharobacter celatus DSM 18785T (92.5 % similarity) and Parvibacter caecicola DSM 22242T (91.2 % similarity).
29583112	6	10	theme	%	1111:1111	arg1	similarity					1113:1122	92.5 % similarity	1106:1122	92.5 % similarity	1106:1122	Based on 16S rRNA gene sequence similarity, the strain was related to Enterorhabdus musicola DSM 19490T (92.9 % similarity), Enterorhabdus caecimuris DSM 21839T (92.7 % similarity), Adlercreutzia equolifaciens DSM 19450T (92.5 % similarity), Asaccharobacter celatus DSM 18785T (92.5 % similarity) and Parvibacter caecicola DSM 22242T (91.2 % similarity).
29583112	5	11	theme	other	813:817	arg1	genera					819:824	other genera	813:824	other genera of this family	813:839	Strain CEBAS 4A belongs to the Eggerthellaceae family and differed from other genera of this family, both phylogenetically and phenotypically.
29583112	8	12	theme	high	1358:1361	arg1	saturated					1331:1339	saturated	1331:1339	saturated	1331:1339	The whole-cell fatty acids were saturated (98.3 %), a very high percentage that differs from the nearest genera ranging from 62 to 73 %.
29583112	8	12	theme	high	1358:1361	arg1	%					1347:1347	98.3 %	1342:1347	98.3 %	1342:1347	The whole-cell fatty acids were saturated (98.3 %), a very high percentage that differs from the nearest genera ranging from 62 to 73 %.
29583112	8	12	theme	high	1358:1361	arg1	acids					1320:1324	The whole-cell fatty acids	1299:1324	The whole-cell fatty acids	1299:1324	The whole-cell fatty acids were saturated (98.3 %), a very high percentage that differs from the nearest genera ranging from 62 to 73 %.
29583112	8	12	theme	high	1358:1361	arg1	percentage					1363:1372	a very high percentage	1351:1372	a very high percentage that differs from the nearest genera ranging from 62 to 73 %	1351:1433	The whole-cell fatty acids were saturated (98.3 %), a very high percentage that differs from the nearest genera ranging from 62 to 73 %.
29583112	4	13	theme	isourolithin	610:621	arg1	A					623:623	isourolithin A	610:623	isourolithin A	610:623	We report here a new bacterium, capable of metabolizing EA into isourolithin A, isolated from healthy human faeces and characterized by determining phenotypic, biochemical and molecular methods.
29583112	6	14	theme	%	1167:1167	arg1	18785T					1154:1159	Asaccharobacter celatus DSM 18785T	1126:1159	Asaccharobacter celatus DSM 18785T (92.5 % similarity)	1126:1179	Based on 16S rRNA gene sequence similarity, the strain was related to Enterorhabdus musicola DSM 19490T (92.9 % similarity), Enterorhabdus caecimuris DSM 21839T (92.7 % similarity), Adlercreutzia equolifaciens DSM 19450T (92.5 % similarity), Asaccharobacter celatus DSM 18785T (92.5 % similarity) and Parvibacter caecicola DSM 22242T (91.2 % similarity).
29583112	6	14	theme	%	1167:1167	arg1	similarity					1169:1178	92.5 % similarity	1162:1178	92.5 % similarity	1162:1178	Based on 16S rRNA gene sequence similarity, the strain was related to Enterorhabdus musicola DSM 19490T (92.9 % similarity), Enterorhabdus caecimuris DSM 21839T (92.7 % similarity), Adlercreutzia equolifaciens DSM 19450T (92.5 % similarity), Asaccharobacter celatus DSM 18785T (92.5 % similarity) and Parvibacter caecicola DSM 22242T (91.2 % similarity).
29583112	11	15	theme	isourolithinifaciens	1854:1873	arg1	sp					1875:1876	Ellagibacter isourolithinifaciens sp	1841:1876	Ellagibacter isourolithinifaciens sp	1841:1876	Based on these data, a new genus, Ellagibacter gen. nov. is proposed with one species, Ellagibacter isourolithinifaciens sp.
29583112	11	15	theme	isourolithinifaciens	1854:1873	arg1	species					1832:1838	one species	1828:1838	one species	1828:1838	Based on these data, a new genus, Ellagibacter gen. nov. is proposed with one species, Ellagibacter isourolithinifaciens sp.
29583112	6	16	theme	gene	902:905	arg1	similarity					916:925	16S rRNA gene sequence similarity	893:925	16S rRNA gene sequence similarity	893:925	Based on 16S rRNA gene sequence similarity, the strain was related to Enterorhabdus musicola DSM 19490T (92.9 % similarity), Enterorhabdus caecimuris DSM 21839T (92.7 % similarity), Adlercreutzia equolifaciens DSM 19450T (92.5 % similarity), Asaccharobacter celatus DSM 18785T (92.5 % similarity) and Parvibacter caecicola DSM 22242T (91.2 % similarity).
29583112	4	17	theme	phenotypic	694:703	arg1	methods					732:738	phenotypic, biochemical and molecular methods	694:738	phenotypic, biochemical and molecular methods	694:738	We report here a new bacterium, capable of metabolizing EA into isourolithin A, isolated from healthy human faeces and characterized by determining phenotypic, biochemical and molecular methods.
29583112	1	18	theme	human	113:117	arg1	gut					119:121	human gut	113:121	human gut	113:121	nov., a new member of the family Eggerthellaceae, isolated from human gut.
29583112	6	19	theme	92.9 	989:993	arg1	similarity					996:1005	92.9 % similarity	989:1005	92.9 % similarity	989:1005	Based on 16S rRNA gene sequence similarity, the strain was related to Enterorhabdus musicola DSM 19490T (92.9 % similarity), Enterorhabdus caecimuris DSM 21839T (92.7 % similarity), Adlercreutzia equolifaciens DSM 19450T (92.5 % similarity), Asaccharobacter celatus DSM 18785T (92.5 % similarity) and Parvibacter caecicola DSM 22242T (91.2 % similarity).
29583112	6	19	theme	92.9 	989:993	arg1	19490T					981:986	Enterorhabdus musicola DSM 19490T	954:986	Enterorhabdus musicola DSM 19490T (92.9 % similarity)	954:1006	Based on 16S rRNA gene sequence similarity, the strain was related to Enterorhabdus musicola DSM 19490T (92.9 % similarity), Enterorhabdus caecimuris DSM 21839T (92.7 % similarity), Adlercreutzia equolifaciens DSM 19450T (92.5 % similarity), Asaccharobacter celatus DSM 18785T (92.5 % similarity) and Parvibacter caecicola DSM 22242T (91.2 % similarity).
29583112	8	20	theme	nearest	1396:1402	arg1	genera					1404:1409	the nearest genera	1392:1409	the nearest genera ranging from 62 to 73 %	1392:1433	The whole-cell fatty acids were saturated (98.3 %), a very high percentage that differs from the nearest genera ranging from 62 to 73 %.
29583112	6	21	theme	DSM	1150:1152	arg1	18785T					1154:1159	Asaccharobacter celatus DSM 18785T	1126:1159	Asaccharobacter celatus DSM 18785T (92.5 % similarity)	1126:1179	Based on 16S rRNA gene sequence similarity, the strain was related to Enterorhabdus musicola DSM 19490T (92.9 % similarity), Enterorhabdus caecimuris DSM 21839T (92.7 % similarity), Adlercreutzia equolifaciens DSM 19450T (92.5 % similarity), Asaccharobacter celatus DSM 18785T (92.5 % similarity) and Parvibacter caecicola DSM 22242T (91.2 % similarity).
29583112	6	21	theme	DSM	1150:1152	arg1	similarity					1169:1178	92.5 % similarity	1162:1178	92.5 % similarity	1162:1178	Based on 16S rRNA gene sequence similarity, the strain was related to Enterorhabdus musicola DSM 19490T (92.9 % similarity), Enterorhabdus caecimuris DSM 21839T (92.7 % similarity), Adlercreutzia equolifaciens DSM 19450T (92.5 % similarity), Asaccharobacter celatus DSM 18785T (92.5 % similarity) and Parvibacter caecicola DSM 22242T (91.2 % similarity).
29583112	10	22	theme	lipid	1633:1637	arg1	profile					1639:1645	the main polar lipid profile	1618:1645	the main polar lipid profile in addition to several phosphoglycolipids (PGL1-2), phospholipids (PL1-4), glycolipids (GL1-6) and lipids	1618:1751	Diphosphatidylglycerol and phosphatidylglycerol comprised the main polar lipid profile in addition to several phosphoglycolipids (PGL1-2), phospholipids (PL1-4), glycolipids (GL1-6) and lipids.
29583112	4	23	theme	human	648:652	arg1	faeces					654:659	healthy human faeces	640:659	healthy human faeces	640:659	We report here a new bacterium, capable of metabolizing EA into isourolithin A, isolated from healthy human faeces and characterized by determining phenotypic, biochemical and molecular methods.
29583112	5	24	theme	CEBAS	748:752	arg1	4A					754:755	Strain CEBAS 4A	741:755	Strain CEBAS 4A	741:755	Strain CEBAS 4A belongs to the Eggerthellaceae family and differed from other genera of this family, both phylogenetically and phenotypically.
29583112	8	25	dep	saturated	1331:1339	arg1	saturated					1331:1339	saturated	1331:1339	saturated	1331:1339	The whole-cell fatty acids were saturated (98.3 %), a very high percentage that differs from the nearest genera ranging from 62 to 73 %.
29583112	8	25	dep	saturated	1331:1339	arg1	%					1347:1347	98.3 %	1342:1347	98.3 %	1342:1347	The whole-cell fatty acids were saturated (98.3 %), a very high percentage that differs from the nearest genera ranging from 62 to 73 %.
29583112	8	25	dep	saturated	1331:1339	arg1	acids					1320:1324	The whole-cell fatty acids	1299:1324	The whole-cell fatty acids	1299:1324	The whole-cell fatty acids were saturated (98.3 %), a very high percentage that differs from the nearest genera ranging from 62 to 73 %.
29583112	8	25	dep	saturated	1331:1339	arg1	percentage					1363:1372	a very high percentage	1351:1372	a very high percentage that differs from the nearest genera ranging from 62 to 73 %	1351:1433	The whole-cell fatty acids were saturated (98.3 %), a very high percentage that differs from the nearest genera ranging from 62 to 73 %.
29583112	1	26	theme	Eggerthellaceae	82:96	arg1	member					61:66	a new member	55:66	a new member of the family Eggerthellaceae	55:96	nov., a new member of the family Eggerthellaceae, isolated from human gut.
29583112	1	26	theme	Eggerthellaceae	82:96	arg1	nov.					49:52	nov.	49:52	nov.	49:52	nov., a new member of the family Eggerthellaceae, isolated from human gut.
29583112	13	27	theme	=DSM	1951:1954	arg1	70284T					1969:1974	=DSM 104140T=CCUG 70284T	1951:1974	=DSM 104140T=CCUG 70284T	1951:1974	The type strain of Ellagibacter isourolithinifaciens is CEBAS 4AT (=DSM 104140T=CCUG 70284T).
29583112	13	27	theme	=DSM	1951:1954	arg1	4AT					1946:1948	CEBAS 4AT	1940:1948	CEBAS 4AT (=DSM 104140T=CCUG 70284T)	1940:1975	The type strain of Ellagibacter isourolithinifaciens is CEBAS 4AT (=DSM 104140T=CCUG 70284T).
29583112	9	28	theme	respiratory	1446:1456	arg1	lipoquinone					1458:1468	The major respiratory lipoquinone	1436:1468	The major respiratory lipoquinone	1436:1468	The major respiratory lipoquinone was menaquinone-7 and the diamino acid in the peptidoglycan was meso-diaminopimelic acid.
29583112	9	28	theme	respiratory	1446:1456	arg1	menaquinone-7					1474:1486	menaquinone-7	1474:1486	menaquinone-7	1474:1486	The major respiratory lipoquinone was menaquinone-7 and the diamino acid in the peptidoglycan was meso-diaminopimelic acid.
29583112	6	29	dep	Enterorhabdus	1009:1021	arg1	caecimuris					1023:1032	caecimuris	1023:1032	caecimuris	1023:1032	Based on 16S rRNA gene sequence similarity, the strain was related to Enterorhabdus musicola DSM 19490T (92.9 % similarity), Enterorhabdus caecimuris DSM 21839T (92.7 % similarity), Adlercreutzia equolifaciens DSM 19450T (92.5 % similarity), Asaccharobacter celatus DSM 18785T (92.5 % similarity) and Parvibacter caecicola DSM 22242T (91.2 % similarity).
29583112	4	30	theme	biochemical	706:716	arg1	methods					732:738	phenotypic, biochemical and molecular methods	694:738	phenotypic, biochemical and molecular methods	694:738	We report here a new bacterium, capable of metabolizing EA into isourolithin A, isolated from healthy human faeces and characterized by determining phenotypic, biochemical and molecular methods.
29583112	11	31	theme	gen.	1801:1804	arg1	genus					1781:1785	a new genus	1775:1785	a new genus	1775:1785	Based on these data, a new genus, Ellagibacter gen. nov. is proposed with one species, Ellagibacter isourolithinifaciens sp.
29583112	11	31	theme	gen.	1801:1804	arg1	nov.					1806:1809	Ellagibacter gen. nov.	1788:1809	Ellagibacter gen. nov.	1788:1809	Based on these data, a new genus, Ellagibacter gen. nov. is proposed with one species, Ellagibacter isourolithinifaciens sp.
29583112	4	32	theme	molecular	722:730	arg1	methods					732:738	phenotypic, biochemical and molecular methods	694:738	phenotypic, biochemical and molecular methods	694:738	We report here a new bacterium, capable of metabolizing EA into isourolithin A, isolated from healthy human faeces and characterized by determining phenotypic, biochemical and molecular methods.
29583112	3	33	theme	final	456:460	arg1	urolithins					481:490	the final and more bioactive urolithins	452:490	the final and more bioactive urolithins	452:490	Gordonibacter species produce some intermediary urolithins but the micro-organisms responsible for the transformation of EA into the final and more bioactive urolithins, such as urolithin A and isourolithin A, are unknown.
29583112	3	33	theme	final	456:460	arg1	A					530:530	isourolithin A	517:530	isourolithin A	517:530	Gordonibacter species produce some intermediary urolithins but the micro-organisms responsible for the transformation of EA into the final and more bioactive urolithins, such as urolithin A and isourolithin A, are unknown.
29583112	3	33	theme	final	456:460	arg1	A					511:511	urolithin A	501:511	urolithin A	501:511	Gordonibacter species produce some intermediary urolithins but the micro-organisms responsible for the transformation of EA into the final and more bioactive urolithins, such as urolithin A and isourolithin A, are unknown.
29583112	0	34	theme	gen.	34:37	arg1	sp					45:46	sp	45:46	sp	45:46	Ellagibacter isourolithinifaciens gen. nov., sp.
29583112	0	34	theme	gen.	34:37	arg1	nov.					39:42	gen. nov.	34:42	gen. nov.	34:42	Ellagibacter isourolithinifaciens gen. nov., sp.
29583112	9	35	theme	diamino	1496:1502	arg1	acid					1504:1507	the diamino acid	1492:1507	the diamino acid in the peptidoglycan	1492:1528	The major respiratory lipoquinone was menaquinone-7 and the diamino acid in the peptidoglycan was meso-diaminopimelic acid.
29583112	9	35	theme	diamino	1496:1502	arg1	acid					1554:1557	meso-diaminopimelic acid	1534:1557	meso-diaminopimelic acid	1534:1557	The major respiratory lipoquinone was menaquinone-7 and the diamino acid in the peptidoglycan was meso-diaminopimelic acid.
29583112	9	36	from	acid	1504:1507	arg1	peptidoglycan					1516:1528	the peptidoglycan	1512:1528	the peptidoglycan	1512:1528	The major respiratory lipoquinone was menaquinone-7 and the diamino acid in the peptidoglycan was meso-diaminopimelic acid.
29583112	3	37	theme	responsible	406:416	arg1	micro-organisms					390:404	the micro-organisms	386:404	the micro-organisms responsible for the transformation of EA into the final and more bioactive urolithins, such as urolithin A and isourolithin A,	386:531	Gordonibacter species produce some intermediary urolithins but the micro-organisms responsible for the transformation of EA into the final and more bioactive urolithins, such as urolithin A and isourolithin A, are unknown.
29583112	3	37	theme	responsible	406:416	arg1	unknown					537:543	unknown	537:543	unknown	537:543	Gordonibacter species produce some intermediary urolithins but the micro-organisms responsible for the transformation of EA into the final and more bioactive urolithins, such as urolithin A and isourolithin A, are unknown.
29583112	10	38	theme	main	1622:1625	arg1	profile					1639:1645	the main polar lipid profile	1618:1645	the main polar lipid profile in addition to several phosphoglycolipids (PGL1-2), phospholipids (PL1-4), glycolipids (GL1-6) and lipids	1618:1751	Diphosphatidylglycerol and phosphatidylglycerol comprised the main polar lipid profile in addition to several phosphoglycolipids (PGL1-2), phospholipids (PL1-4), glycolipids (GL1-6) and lipids.
29583112	2	39	theme	gut	139:141	arg1	metabolites					153:163	gut microbial metabolites	139:163	gut microbial metabolites that exert health benefits in vivo and are generated from ellagic acid (EA) and ellagitannin-containing foods such as strawberries, pomegranates and walnuts	139:320	Urolithins are gut microbial metabolites that exert health benefits in vivo and are generated from ellagic acid (EA) and ellagitannin-containing foods such as strawberries, pomegranates and walnuts.
29583112	2	39	theme	gut	139:141	arg1	Urolithins					124:133	Urolithins	124:133	Urolithins	124:133	Urolithins are gut microbial metabolites that exert health benefits in vivo and are generated from ellagic acid (EA) and ellagitannin-containing foods such as strawberries, pomegranates and walnuts.
29583112	13	40	theme	CEBAS	1940:1944	arg1	70284T					1969:1974	=DSM 104140T=CCUG 70284T	1951:1974	=DSM 104140T=CCUG 70284T	1951:1974	The type strain of Ellagibacter isourolithinifaciens is CEBAS 4AT (=DSM 104140T=CCUG 70284T).
29583112	13	40	theme	CEBAS	1940:1944	arg1	strain					1893:1898	The type strain	1884:1898	The type strain of Ellagibacter isourolithinifaciens	1884:1935	The type strain of Ellagibacter isourolithinifaciens is CEBAS 4AT (=DSM 104140T=CCUG 70284T).
29583112	13	40	theme	CEBAS	1940:1944	arg1	4AT					1946:1948	CEBAS 4AT	1940:1948	CEBAS 4AT (=DSM 104140T=CCUG 70284T)	1940:1975	The type strain of Ellagibacter isourolithinifaciens is CEBAS 4AT (=DSM 104140T=CCUG 70284T).
29583112	6	41	dep	Adlercreutzia	1066:1078	arg1	equolifaciens					1080:1092	equolifaciens	1080:1092	equolifaciens	1080:1092	Based on 16S rRNA gene sequence similarity, the strain was related to Enterorhabdus musicola DSM 19490T (92.9 % similarity), Enterorhabdus caecimuris DSM 21839T (92.7 % similarity), Adlercreutzia equolifaciens DSM 19450T (92.5 % similarity), Asaccharobacter celatus DSM 18785T (92.5 % similarity) and Parvibacter caecicola DSM 22242T (91.2 % similarity).
29583112	9	42	theme	meso-diaminopimelic	1534:1552	arg1	acid					1504:1507	the diamino acid	1492:1507	the diamino acid in the peptidoglycan	1492:1528	The major respiratory lipoquinone was menaquinone-7 and the diamino acid in the peptidoglycan was meso-diaminopimelic acid.
29583112	9	42	theme	meso-diaminopimelic	1534:1552	arg1	acid					1554:1557	meso-diaminopimelic acid	1534:1557	meso-diaminopimelic acid	1534:1557	The major respiratory lipoquinone was menaquinone-7 and the diamino acid in the peptidoglycan was meso-diaminopimelic acid.
29583112	3	43	theme	urolithin	501:509	arg1	A					511:511	urolithin A	501:511	urolithin A	501:511	Gordonibacter species produce some intermediary urolithins but the micro-organisms responsible for the transformation of EA into the final and more bioactive urolithins, such as urolithin A and isourolithin A, are unknown.
29583112	6	44	theme	rRNA	897:900	arg1	similarity					916:925	16S rRNA gene sequence similarity	893:925	16S rRNA gene sequence similarity	893:925	Based on 16S rRNA gene sequence similarity, the strain was related to Enterorhabdus musicola DSM 19490T (92.9 % similarity), Enterorhabdus caecimuris DSM 21839T (92.7 % similarity), Adlercreutzia equolifaciens DSM 19450T (92.5 % similarity), Asaccharobacter celatus DSM 18785T (92.5 % similarity) and Parvibacter caecicola DSM 22242T (91.2 % similarity).
29583112	4	45	theme	capable	578:584	arg1	bacterium					567:575	a new bacterium	561:575	a new bacterium	561:575	We report here a new bacterium, capable of metabolizing EA into isourolithin A, isolated from healthy human faeces and characterized by determining phenotypic, biochemical and molecular methods.
29583112	6	46	theme	%	1224:1224	arg1	similarity					1226:1235	91.2 % similarity	1219:1235	91.2 % similarity	1219:1235	Based on 16S rRNA gene sequence similarity, the strain was related to Enterorhabdus musicola DSM 19490T (92.9 % similarity), Enterorhabdus caecimuris DSM 21839T (92.7 % similarity), Adlercreutzia equolifaciens DSM 19450T (92.5 % similarity), Asaccharobacter celatus DSM 18785T (92.5 % similarity) and Parvibacter caecicola DSM 22242T (91.2 % similarity).
29583112	6	46	theme	%	1224:1224	arg1	22242T					1211:1216	Parvibacter caecicola DSM 22242T	1185:1216	Parvibacter caecicola DSM 22242T (91.2 % similarity)	1185:1236	Based on 16S rRNA gene sequence similarity, the strain was related to Enterorhabdus musicola DSM 19490T (92.9 % similarity), Enterorhabdus caecimuris DSM 21839T (92.7 % similarity), Adlercreutzia equolifaciens DSM 19450T (92.5 % similarity), Asaccharobacter celatus DSM 18785T (92.5 % similarity) and Parvibacter caecicola DSM 22242T (91.2 % similarity).
29583112	13	47	theme	isourolithinifaciens	1916:1935	arg1	strain					1893:1898	The type strain	1884:1898	The type strain of Ellagibacter isourolithinifaciens	1884:1935	The type strain of Ellagibacter isourolithinifaciens is CEBAS 4AT (=DSM 104140T=CCUG 70284T).
29583112	13	47	theme	isourolithinifaciens	1916:1935	arg1	4AT					1946:1948	CEBAS 4AT	1940:1948	CEBAS 4AT (=DSM 104140T=CCUG 70284T)	1940:1975	The type strain of Ellagibacter isourolithinifaciens is CEBAS 4AT (=DSM 104140T=CCUG 70284T).
29583112	11	48	theme	new	1777:1779	arg1	genus					1781:1785	a new genus	1775:1785	a new genus	1775:1785	Based on these data, a new genus, Ellagibacter gen. nov. is proposed with one species, Ellagibacter isourolithinifaciens sp.
29583112	11	48	theme	new	1777:1779	arg1	nov.					1806:1809	Ellagibacter gen. nov.	1788:1809	Ellagibacter gen. nov.	1788:1809	Based on these data, a new genus, Ellagibacter gen. nov. is proposed with one species, Ellagibacter isourolithinifaciens sp.
29583112	6	49	theme	92.7 	1046:1050	arg1	similarity					1053:1062	92.7 % similarity	1046:1062	92.7 % similarity	1046:1062	Based on 16S rRNA gene sequence similarity, the strain was related to Enterorhabdus musicola DSM 19490T (92.9 % similarity), Enterorhabdus caecimuris DSM 21839T (92.7 % similarity), Adlercreutzia equolifaciens DSM 19450T (92.5 % similarity), Asaccharobacter celatus DSM 18785T (92.5 % similarity) and Parvibacter caecicola DSM 22242T (91.2 % similarity).
29583112	6	49	theme	92.7 	1046:1050	arg1	21839T					1038:1043	Enterorhabdus caecimuris DSM 21839T	1009:1043	Enterorhabdus caecimuris DSM 21839T (92.7 % similarity)	1009:1063	Based on 16S rRNA gene sequence similarity, the strain was related to Enterorhabdus musicola DSM 19490T (92.9 % similarity), Enterorhabdus caecimuris DSM 21839T (92.7 % similarity), Adlercreutzia equolifaciens DSM 19450T (92.5 % similarity), Asaccharobacter celatus DSM 18785T (92.5 % similarity) and Parvibacter caecicola DSM 22242T (91.2 % similarity).
29583112	2	50	theme	ellagitannin-containing	245:267	arg1	walnuts					314:320	walnuts	314:320	walnuts	314:320	Urolithins are gut microbial metabolites that exert health benefits in vivo and are generated from ellagic acid (EA) and ellagitannin-containing foods such as strawberries, pomegranates and walnuts.
29583112	2	50	theme	ellagitannin-containing	245:267	arg1	pomegranates					297:308	pomegranates	297:308	pomegranates	297:308	Urolithins are gut microbial metabolites that exert health benefits in vivo and are generated from ellagic acid (EA) and ellagitannin-containing foods such as strawberries, pomegranates and walnuts.
29583112	2	50	theme	ellagitannin-containing	245:267	arg1	foods					269:273	ellagitannin-containing foods	245:273	ellagitannin-containing foods such as strawberries, pomegranates and walnuts	245:320	Urolithins are gut microbial metabolites that exert health benefits in vivo and are generated from ellagic acid (EA) and ellagitannin-containing foods such as strawberries, pomegranates and walnuts.
29583112	2	50	theme	ellagitannin-containing	245:267	arg1	strawberries					283:294	strawberries	283:294	strawberries	283:294	Urolithins are gut microbial metabolites that exert health benefits in vivo and are generated from ellagic acid (EA) and ellagitannin-containing foods such as strawberries, pomegranates and walnuts.
29583112	6	51	theme	DSM	1207:1209	arg1	similarity					1226:1235	91.2 % similarity	1219:1235	91.2 % similarity	1219:1235	Based on 16S rRNA gene sequence similarity, the strain was related to Enterorhabdus musicola DSM 19490T (92.9 % similarity), Enterorhabdus caecimuris DSM 21839T (92.7 % similarity), Adlercreutzia equolifaciens DSM 19450T (92.5 % similarity), Asaccharobacter celatus DSM 18785T (92.5 % similarity) and Parvibacter caecicola DSM 22242T (91.2 % similarity).
29583112	6	51	theme	DSM	1207:1209	arg1	22242T					1211:1216	Parvibacter caecicola DSM 22242T	1185:1216	Parvibacter caecicola DSM 22242T (91.2 % similarity)	1185:1236	Based on 16S rRNA gene sequence similarity, the strain was related to Enterorhabdus musicola DSM 19490T (92.9 % similarity), Enterorhabdus caecimuris DSM 21839T (92.7 % similarity), Adlercreutzia equolifaciens DSM 19450T (92.5 % similarity), Asaccharobacter celatus DSM 18785T (92.5 % similarity) and Parvibacter caecicola DSM 22242T (91.2 % similarity).
29583112	13	52	theme	type	1888:1891	arg1	strain					1893:1898	The type strain	1884:1898	The type strain of Ellagibacter isourolithinifaciens	1884:1935	The type strain of Ellagibacter isourolithinifaciens is CEBAS 4AT (=DSM 104140T=CCUG 70284T).
29583112	13	52	theme	type	1888:1891	arg1	4AT					1946:1948	CEBAS 4AT	1940:1948	CEBAS 4AT (=DSM 104140T=CCUG 70284T)	1940:1975	The type strain of Ellagibacter isourolithinifaciens is CEBAS 4AT (=DSM 104140T=CCUG 70284T).
29583112	6	53	theme	Parvibacter	1185:1195	arg1	similarity					1226:1235	91.2 % similarity	1219:1235	91.2 % similarity	1219:1235	Based on 16S rRNA gene sequence similarity, the strain was related to Enterorhabdus musicola DSM 19490T (92.9 % similarity), Enterorhabdus caecimuris DSM 21839T (92.7 % similarity), Adlercreutzia equolifaciens DSM 19450T (92.5 % similarity), Asaccharobacter celatus DSM 18785T (92.5 % similarity) and Parvibacter caecicola DSM 22242T (91.2 % similarity).
29583112	6	53	theme	Parvibacter	1185:1195	arg1	22242T					1211:1216	Parvibacter caecicola DSM 22242T	1185:1216	Parvibacter caecicola DSM 22242T (91.2 % similarity)	1185:1236	Based on 16S rRNA gene sequence similarity, the strain was related to Enterorhabdus musicola DSM 19490T (92.9 % similarity), Enterorhabdus caecimuris DSM 21839T (92.7 % similarity), Adlercreutzia equolifaciens DSM 19450T (92.5 % similarity), Asaccharobacter celatus DSM 18785T (92.5 % similarity) and Parvibacter caecicola DSM 22242T (91.2 % similarity).
29583112	6	54	theme	92.5 	1106:1110	arg1	19450T					1098:1103	Adlercreutzia equolifaciens DSM 19450T	1066:1103	Adlercreutzia equolifaciens DSM 19450T (92.5 % similarity)	1066:1123	Based on 16S rRNA gene sequence similarity, the strain was related to Enterorhabdus musicola DSM 19490T (92.9 % similarity), Enterorhabdus caecimuris DSM 21839T (92.7 % similarity), Adlercreutzia equolifaciens DSM 19450T (92.5 % similarity), Asaccharobacter celatus DSM 18785T (92.5 % similarity) and Parvibacter caecicola DSM 22242T (91.2 % similarity).
29583112	6	54	theme	92.5 	1106:1110	arg1	similarity					1113:1122	92.5 % similarity	1106:1122	92.5 % similarity	1106:1122	Based on 16S rRNA gene sequence similarity, the strain was related to Enterorhabdus musicola DSM 19490T (92.9 % similarity), Enterorhabdus caecimuris DSM 21839T (92.7 % similarity), Adlercreutzia equolifaciens DSM 19450T (92.5 % similarity), Asaccharobacter celatus DSM 18785T (92.5 % similarity) and Parvibacter caecicola DSM 22242T (91.2 % similarity).
29583112	11	55	theme	Ellagibacter	1841:1852	arg1	sp					1875:1876	Ellagibacter isourolithinifaciens sp	1841:1876	Ellagibacter isourolithinifaciens sp	1841:1876	Based on these data, a new genus, Ellagibacter gen. nov. is proposed with one species, Ellagibacter isourolithinifaciens sp.
29583112	11	55	theme	Ellagibacter	1841:1852	arg1	species					1832:1838	one species	1828:1838	one species	1828:1838	Based on these data, a new genus, Ellagibacter gen. nov. is proposed with one species, Ellagibacter isourolithinifaciens sp.
29583112	6	56	theme	sequence	907:914	arg1	similarity					916:925	16S rRNA gene sequence similarity	893:925	16S rRNA gene sequence similarity	893:925	Based on 16S rRNA gene sequence similarity, the strain was related to Enterorhabdus musicola DSM 19490T (92.9 % similarity), Enterorhabdus caecimuris DSM 21839T (92.7 % similarity), Adlercreutzia equolifaciens DSM 19450T (92.5 % similarity), Asaccharobacter celatus DSM 18785T (92.5 % similarity) and Parvibacter caecicola DSM 22242T (91.2 % similarity).
29583112	6	57	theme	92.5 	1162:1166	arg1	18785T					1154:1159	Asaccharobacter celatus DSM 18785T	1126:1159	Asaccharobacter celatus DSM 18785T (92.5 % similarity)	1126:1179	Based on 16S rRNA gene sequence similarity, the strain was related to Enterorhabdus musicola DSM 19490T (92.9 % similarity), Enterorhabdus caecimuris DSM 21839T (92.7 % similarity), Adlercreutzia equolifaciens DSM 19450T (92.5 % similarity), Asaccharobacter celatus DSM 18785T (92.5 % similarity) and Parvibacter caecicola DSM 22242T (91.2 % similarity).
29583112	6	57	theme	92.5 	1162:1166	arg1	similarity					1169:1178	92.5 % similarity	1162:1178	92.5 % similarity	1162:1178	Based on 16S rRNA gene sequence similarity, the strain was related to Enterorhabdus musicola DSM 19490T (92.9 % similarity), Enterorhabdus caecimuris DSM 21839T (92.7 % similarity), Adlercreutzia equolifaciens DSM 19450T (92.5 % similarity), Asaccharobacter celatus DSM 18785T (92.5 % similarity) and Parvibacter caecicola DSM 22242T (91.2 % similarity).
29583112	6	58	theme	%	994:994	arg1	similarity					996:1005	92.9 % similarity	989:1005	92.9 % similarity	989:1005	Based on 16S rRNA gene sequence similarity, the strain was related to Enterorhabdus musicola DSM 19490T (92.9 % similarity), Enterorhabdus caecimuris DSM 21839T (92.7 % similarity), Adlercreutzia equolifaciens DSM 19450T (92.5 % similarity), Asaccharobacter celatus DSM 18785T (92.5 % similarity) and Parvibacter caecicola DSM 22242T (91.2 % similarity).
29583112	6	58	theme	%	994:994	arg1	19490T					981:986	Enterorhabdus musicola DSM 19490T	954:986	Enterorhabdus musicola DSM 19490T (92.9 % similarity)	954:1006	Based on 16S rRNA gene sequence similarity, the strain was related to Enterorhabdus musicola DSM 19490T (92.9 % similarity), Enterorhabdus caecimuris DSM 21839T (92.7 % similarity), Adlercreutzia equolifaciens DSM 19450T (92.5 % similarity), Asaccharobacter celatus DSM 18785T (92.5 % similarity) and Parvibacter caecicola DSM 22242T (91.2 % similarity).
29583112	4	59	dep	bacterium	567:575	arg1	isolated					626:633	isolated	626:633	isolated from healthy human faeces	626:659	We report here a new bacterium, capable of metabolizing EA into isourolithin A, isolated from healthy human faeces and characterized by determining phenotypic, biochemical and molecular methods.
29583112	4	59	dep	bacterium	567:575	arg1	characterized					665:677	characterized	665:677	characterized by determining phenotypic, biochemical and molecular methods	665:738	We report here a new bacterium, capable of metabolizing EA into isourolithin A, isolated from healthy human faeces and characterized by determining phenotypic, biochemical and molecular methods.
29583112	2	60	theme	ellagic	223:229	arg1	EA					237:238	EA	237:238	EA	237:238	Urolithins are gut microbial metabolites that exert health benefits in vivo and are generated from ellagic acid (EA) and ellagitannin-containing foods such as strawberries, pomegranates and walnuts.
29583112	2	60	theme	ellagic	223:229	arg1	acid					231:234	ellagic acid	223:234	ellagic acid (EA)	223:239	Urolithins are gut microbial metabolites that exert health benefits in vivo and are generated from ellagic acid (EA) and ellagitannin-containing foods such as strawberries, pomegranates and walnuts.
29583112	6	61	dep	Enterorhabdus	954:966	arg1	musicola					968:975	musicola	968:975	musicola	968:975	Based on 16S rRNA gene sequence similarity, the strain was related to Enterorhabdus musicola DSM 19490T (92.9 % similarity), Enterorhabdus caecimuris DSM 21839T (92.7 % similarity), Adlercreutzia equolifaciens DSM 19450T (92.5 % similarity), Asaccharobacter celatus DSM 18785T (92.5 % similarity) and Parvibacter caecicola DSM 22242T (91.2 % similarity).
29583112	6	62	theme	celatus	1142:1148	arg1	18785T					1154:1159	Asaccharobacter celatus DSM 18785T	1126:1159	Asaccharobacter celatus DSM 18785T (92.5 % similarity)	1126:1179	Based on 16S rRNA gene sequence similarity, the strain was related to Enterorhabdus musicola DSM 19490T (92.9 % similarity), Enterorhabdus caecimuris DSM 21839T (92.7 % similarity), Adlercreutzia equolifaciens DSM 19450T (92.5 % similarity), Asaccharobacter celatus DSM 18785T (92.5 % similarity) and Parvibacter caecicola DSM 22242T (91.2 % similarity).
29583112	6	62	theme	celatus	1142:1148	arg1	similarity					1169:1178	92.5 % similarity	1162:1178	92.5 % similarity	1162:1178	Based on 16S rRNA gene sequence similarity, the strain was related to Enterorhabdus musicola DSM 19490T (92.9 % similarity), Enterorhabdus caecimuris DSM 21839T (92.7 % similarity), Adlercreutzia equolifaciens DSM 19450T (92.5 % similarity), Asaccharobacter celatus DSM 18785T (92.5 % similarity) and Parvibacter caecicola DSM 22242T (91.2 % similarity).
29583112	4	63	theme	healthy	640:646	arg1	faeces					654:659	healthy human faeces	640:659	healthy human faeces	640:659	We report here a new bacterium, capable of metabolizing EA into isourolithin A, isolated from healthy human faeces and characterized by determining phenotypic, biochemical and molecular methods.
29583112	5	64	theme	Strain	741:746	arg1	4A					754:755	Strain CEBAS 4A	741:755	Strain CEBAS 4A	741:755	Strain CEBAS 4A belongs to the Eggerthellaceae family and differed from other genera of this family, both phylogenetically and phenotypically.
29583112	6	65	theme	DSM	977:979	arg1	similarity					996:1005	92.9 % similarity	989:1005	92.9 % similarity	989:1005	Based on 16S rRNA gene sequence similarity, the strain was related to Enterorhabdus musicola DSM 19490T (92.9 % similarity), Enterorhabdus caecimuris DSM 21839T (92.7 % similarity), Adlercreutzia equolifaciens DSM 19450T (92.5 % similarity), Asaccharobacter celatus DSM 18785T (92.5 % similarity) and Parvibacter caecicola DSM 22242T (91.2 % similarity).
29583112	6	65	theme	DSM	977:979	arg1	19490T					981:986	Enterorhabdus musicola DSM 19490T	954:986	Enterorhabdus musicola DSM 19490T (92.9 % similarity)	954:1006	Based on 16S rRNA gene sequence similarity, the strain was related to Enterorhabdus musicola DSM 19490T (92.9 % similarity), Enterorhabdus caecimuris DSM 21839T (92.7 % similarity), Adlercreutzia equolifaciens DSM 19450T (92.5 % similarity), Asaccharobacter celatus DSM 18785T (92.5 % similarity) and Parvibacter caecicola DSM 22242T (91.2 % similarity).
29583112	9	66	theme	major	1440:1444	arg1	lipoquinone					1458:1468	The major respiratory lipoquinone	1436:1468	The major respiratory lipoquinone	1436:1468	The major respiratory lipoquinone was menaquinone-7 and the diamino acid in the peptidoglycan was meso-diaminopimelic acid.
29583112	9	66	theme	major	1440:1444	arg1	menaquinone-7					1474:1486	menaquinone-7	1474:1486	menaquinone-7	1474:1486	The major respiratory lipoquinone was menaquinone-7 and the diamino acid in the peptidoglycan was meso-diaminopimelic acid.
29583112	6	67	theme	Enterorhabdus	954:966	arg1	similarity					996:1005	92.9 % similarity	989:1005	92.9 % similarity	989:1005	Based on 16S rRNA gene sequence similarity, the strain was related to Enterorhabdus musicola DSM 19490T (92.9 % similarity), Enterorhabdus caecimuris DSM 21839T (92.7 % similarity), Adlercreutzia equolifaciens DSM 19450T (92.5 % similarity), Asaccharobacter celatus DSM 18785T (92.5 % similarity) and Parvibacter caecicola DSM 22242T (91.2 % similarity).
29583112	6	67	theme	Enterorhabdus	954:966	arg1	19490T					981:986	Enterorhabdus musicola DSM 19490T	954:986	Enterorhabdus musicola DSM 19490T (92.9 % similarity)	954:1006	Based on 16S rRNA gene sequence similarity, the strain was related to Enterorhabdus musicola DSM 19490T (92.9 % similarity), Enterorhabdus caecimuris DSM 21839T (92.7 % similarity), Adlercreutzia equolifaciens DSM 19450T (92.5 % similarity), Asaccharobacter celatus DSM 18785T (92.5 % similarity) and Parvibacter caecicola DSM 22242T (91.2 % similarity).
29583112	13	68	theme	104140T=CCUG	1956:1967	arg1	70284T					1969:1974	=DSM 104140T=CCUG 70284T	1951:1974	=DSM 104140T=CCUG 70284T	1951:1974	The type strain of Ellagibacter isourolithinifaciens is CEBAS 4AT (=DSM 104140T=CCUG 70284T).
29583112	13	68	theme	104140T=CCUG	1956:1967	arg1	4AT					1946:1948	CEBAS 4AT	1940:1948	CEBAS 4AT (=DSM 104140T=CCUG 70284T)	1940:1975	The type strain of Ellagibacter isourolithinifaciens is CEBAS 4AT (=DSM 104140T=CCUG 70284T).
29583112	1	69	theme	new	57:59	arg1	member					61:66	a new member	55:66	a new member of the family Eggerthellaceae	55:96	nov., a new member of the family Eggerthellaceae, isolated from human gut.
29583112	1	69	theme	new	57:59	arg1	nov.					49:52	nov.	49:52	nov.	49:52	nov., a new member of the family Eggerthellaceae, isolated from human gut.
29583112	3	70	theme	Gordonibacter	323:335	arg1	species					337:343	Gordonibacter species	323:343	Gordonibacter species	323:343	Gordonibacter species produce some intermediary urolithins but the micro-organisms responsible for the transformation of EA into the final and more bioactive urolithins, such as urolithin A and isourolithin A, are unknown.
29583112	3	71	theme	EA	444:445	arg1	transformation					426:439	the transformation	422:439	the transformation of EA into the final and more bioactive urolithins, such as urolithin A and isourolithin A,	422:531	Gordonibacter species produce some intermediary urolithins but the micro-organisms responsible for the transformation of EA into the final and more bioactive urolithins, such as urolithin A and isourolithin A, are unknown.
29583112	5	72	theme	Eggerthellaceae	772:786	arg1	family					788:793	the Eggerthellaceae family	768:793	the Eggerthellaceae family	768:793	Strain CEBAS 4A belongs to the Eggerthellaceae family and differed from other genera of this family, both phylogenetically and phenotypically.
29583112	6	73	theme	DSM	1034:1036	arg1	similarity					1053:1062	92.7 % similarity	1046:1062	92.7 % similarity	1046:1062	Based on 16S rRNA gene sequence similarity, the strain was related to Enterorhabdus musicola DSM 19490T (92.9 % similarity), Enterorhabdus caecimuris DSM 21839T (92.7 % similarity), Adlercreutzia equolifaciens DSM 19450T (92.5 % similarity), Asaccharobacter celatus DSM 18785T (92.5 % similarity) and Parvibacter caecicola DSM 22242T (91.2 % similarity).
29583112	6	73	theme	DSM	1034:1036	arg1	21839T					1038:1043	Enterorhabdus caecimuris DSM 21839T	1009:1043	Enterorhabdus caecimuris DSM 21839T (92.7 % similarity)	1009:1063	Based on 16S rRNA gene sequence similarity, the strain was related to Enterorhabdus musicola DSM 19490T (92.9 % similarity), Enterorhabdus caecimuris DSM 21839T (92.7 % similarity), Adlercreutzia equolifaciens DSM 19450T (92.5 % similarity), Asaccharobacter celatus DSM 18785T (92.5 % similarity) and Parvibacter caecicola DSM 22242T (91.2 % similarity).
29583112	8	74	theme	whole-cell	1303:1312	arg1	saturated					1331:1339	saturated	1331:1339	saturated	1331:1339	The whole-cell fatty acids were saturated (98.3 %), a very high percentage that differs from the nearest genera ranging from 62 to 73 %.
29583112	8	74	theme	whole-cell	1303:1312	arg1	%					1347:1347	98.3 %	1342:1347	98.3 %	1342:1347	The whole-cell fatty acids were saturated (98.3 %), a very high percentage that differs from the nearest genera ranging from 62 to 73 %.
29583112	8	74	theme	whole-cell	1303:1312	arg1	acids					1320:1324	The whole-cell fatty acids	1299:1324	The whole-cell fatty acids	1299:1324	The whole-cell fatty acids were saturated (98.3 %), a very high percentage that differs from the nearest genera ranging from 62 to 73 %.
29583112	8	74	theme	whole-cell	1303:1312	arg1	percentage					1363:1372	a very high percentage	1351:1372	a very high percentage that differs from the nearest genera ranging from 62 to 73 %	1351:1433	The whole-cell fatty acids were saturated (98.3 %), a very high percentage that differs from the nearest genera ranging from 62 to 73 %.
29583112	1	75	theme	family	75:80	arg1	Eggerthellaceae					82:96	the family Eggerthellaceae	71:96	the family Eggerthellaceae	71:96	nov., a new member of the family Eggerthellaceae, isolated from human gut.
29583112	6	76	theme	Enterorhabdus	1009:1021	arg1	similarity					1053:1062	92.7 % similarity	1046:1062	92.7 % similarity	1046:1062	Based on 16S rRNA gene sequence similarity, the strain was related to Enterorhabdus musicola DSM 19490T (92.9 % similarity), Enterorhabdus caecimuris DSM 21839T (92.7 % similarity), Adlercreutzia equolifaciens DSM 19450T (92.5 % similarity), Asaccharobacter celatus DSM 18785T (92.5 % similarity) and Parvibacter caecicola DSM 22242T (91.2 % similarity).
29583112	6	76	theme	Enterorhabdus	1009:1021	arg1	21839T					1038:1043	Enterorhabdus caecimuris DSM 21839T	1009:1043	Enterorhabdus caecimuris DSM 21839T (92.7 % similarity)	1009:1063	Based on 16S rRNA gene sequence similarity, the strain was related to Enterorhabdus musicola DSM 19490T (92.9 % similarity), Enterorhabdus caecimuris DSM 21839T (92.7 % similarity), Adlercreutzia equolifaciens DSM 19450T (92.5 % similarity), Asaccharobacter celatus DSM 18785T (92.5 % similarity) and Parvibacter caecicola DSM 22242T (91.2 % similarity).
29583112	3	77	theme	intermediary	358:369	arg1	urolithins					371:380	some intermediary urolithins	353:380	some intermediary urolithins	353:380	Gordonibacter species produce some intermediary urolithins but the micro-organisms responsible for the transformation of EA into the final and more bioactive urolithins, such as urolithin A and isourolithin A, are unknown.
29583112	10	78	theme	polar	1627:1631	arg1	profile					1639:1645	the main polar lipid profile	1618:1645	the main polar lipid profile in addition to several phosphoglycolipids (PGL1-2), phospholipids (PL1-4), glycolipids (GL1-6) and lipids	1618:1751	Diphosphatidylglycerol and phosphatidylglycerol comprised the main polar lipid profile in addition to several phosphoglycolipids (PGL1-2), phospholipids (PL1-4), glycolipids (GL1-6) and lipids.
29583112	2	79	theme	microbial	143:151	arg1	metabolites					153:163	gut microbial metabolites	139:163	gut microbial metabolites that exert health benefits in vivo and are generated from ellagic acid (EA) and ellagitannin-containing foods such as strawberries, pomegranates and walnuts	139:320	Urolithins are gut microbial metabolites that exert health benefits in vivo and are generated from ellagic acid (EA) and ellagitannin-containing foods such as strawberries, pomegranates and walnuts.
29583112	2	79	theme	microbial	143:151	arg1	Urolithins					124:133	Urolithins	124:133	Urolithins	124:133	Urolithins are gut microbial metabolites that exert health benefits in vivo and are generated from ellagic acid (EA) and ellagitannin-containing foods such as strawberries, pomegranates and walnuts.
29583112	6	80	theme	DSM	1094:1096	arg1	19450T					1098:1103	Adlercreutzia equolifaciens DSM 19450T	1066:1103	Adlercreutzia equolifaciens DSM 19450T (92.5 % similarity)	1066:1123	Based on 16S rRNA gene sequence similarity, the strain was related to Enterorhabdus musicola DSM 19490T (92.9 % similarity), Enterorhabdus caecimuris DSM 21839T (92.7 % similarity), Adlercreutzia equolifaciens DSM 19450T (92.5 % similarity), Asaccharobacter celatus DSM 18785T (92.5 % similarity) and Parvibacter caecicola DSM 22242T (91.2 % similarity).
29583112	6	80	theme	DSM	1094:1096	arg1	similarity					1113:1122	92.5 % similarity	1106:1122	92.5 % similarity	1106:1122	Based on 16S rRNA gene sequence similarity, the strain was related to Enterorhabdus musicola DSM 19490T (92.9 % similarity), Enterorhabdus caecimuris DSM 21839T (92.7 % similarity), Adlercreutzia equolifaciens DSM 19450T (92.5 % similarity), Asaccharobacter celatus DSM 18785T (92.5 % similarity) and Parvibacter caecicola DSM 22242T (91.2 % similarity).
29583112	6	81	theme	Adlercreutzia	1066:1078	arg1	19450T					1098:1103	Adlercreutzia equolifaciens DSM 19450T	1066:1103	Adlercreutzia equolifaciens DSM 19450T (92.5 % similarity)	1066:1123	Based on 16S rRNA gene sequence similarity, the strain was related to Enterorhabdus musicola DSM 19490T (92.9 % similarity), Enterorhabdus caecimuris DSM 21839T (92.7 % similarity), Adlercreutzia equolifaciens DSM 19450T (92.5 % similarity), Asaccharobacter celatus DSM 18785T (92.5 % similarity) and Parvibacter caecicola DSM 22242T (91.2 % similarity).
29583112	6	81	theme	Adlercreutzia	1066:1078	arg1	similarity					1113:1122	92.5 % similarity	1106:1122	92.5 % similarity	1106:1122	Based on 16S rRNA gene sequence similarity, the strain was related to Enterorhabdus musicola DSM 19490T (92.9 % similarity), Enterorhabdus caecimuris DSM 21839T (92.7 % similarity), Adlercreutzia equolifaciens DSM 19450T (92.5 % similarity), Asaccharobacter celatus DSM 18785T (92.5 % similarity) and Parvibacter caecicola DSM 22242T (91.2 % similarity).
29583112	2	82	theme	health	176:181	arg1	benefits					183:190	health benefits	176:190	health benefits	176:190	Urolithins are gut microbial metabolites that exert health benefits in vivo and are generated from ellagic acid (EA) and ellagitannin-containing foods such as strawberries, pomegranates and walnuts.
29583112	3	83	theme	bioactive	471:479	arg1	urolithins					481:490	the final and more bioactive urolithins	452:490	the final and more bioactive urolithins	452:490	Gordonibacter species produce some intermediary urolithins but the micro-organisms responsible for the transformation of EA into the final and more bioactive urolithins, such as urolithin A and isourolithin A, are unknown.
29583112	3	83	theme	bioactive	471:479	arg1	A					530:530	isourolithin A	517:530	isourolithin A	517:530	Gordonibacter species produce some intermediary urolithins but the micro-organisms responsible for the transformation of EA into the final and more bioactive urolithins, such as urolithin A and isourolithin A, are unknown.
29583112	3	83	theme	bioactive	471:479	arg1	A					511:511	urolithin A	501:511	urolithin A	501:511	Gordonibacter species produce some intermediary urolithins but the micro-organisms responsible for the transformation of EA into the final and more bioactive urolithins, such as urolithin A and isourolithin A, are unknown.
29583112	13	84	theme	Ellagibacter	1903:1914	arg1	isourolithinifaciens					1916:1935	Ellagibacter isourolithinifaciens	1903:1935	Ellagibacter isourolithinifaciens	1903:1935	The type strain of Ellagibacter isourolithinifaciens is CEBAS 4AT (=DSM 104140T=CCUG 70284T).
29583112	11	85	theme	Ellagibacter	1788:1799	arg1	genus					1781:1785	a new genus	1775:1785	a new genus	1775:1785	Based on these data, a new genus, Ellagibacter gen. nov. is proposed with one species, Ellagibacter isourolithinifaciens sp.
29583112	11	85	theme	Ellagibacter	1788:1799	arg1	nov.					1806:1809	Ellagibacter gen. nov.	1788:1809	Ellagibacter gen. nov.	1788:1809	Based on these data, a new genus, Ellagibacter gen. nov. is proposed with one species, Ellagibacter isourolithinifaciens sp.
29583112	8	86	dep	73 	1430:1432	arg1	to					1427:1428	to	1427:1428	to	1427:1428	The whole-cell fatty acids were saturated (98.3 %), a very high percentage that differs from the nearest genera ranging from 62 to 73 %.
29543147	3	0	theme	Khao	266:269	arg1	district					282:289	Khao Khitchakut district	266:289	Khao Khitchakut district	266:289	at Khao Khitchakut district, Chantaburi province, Thailand, was characterized by using a polyphasic approach.
29543147	12	1	theme	rRNA	1098:1101	arg1	analysis					1117:1124	16S rRNA gene sequence analysis	1094:1124	16S rRNA gene sequence analysis	1094:1124	Based on 16S rRNA gene sequence analysis, strain GKU 128T was closely related to the type strains of Actinomadura nitritigenes NBRC 15918T (99.2 % sequence similarity) and Actinomadura fibrosa JCM 9371T (98.7 %).
29543147	5	2	from	presence	553:560	arg1	peptidoglycan					622:634	the peptidoglycan	618:634	the peptidoglycan	618:634	Analysis of the cell wall revealed the presence of meso-diaminopimelic acid and N-acetylmuramic acid in the peptidoglycan.
29543147	5	3	attach	presence	553:560	arg2	acid					585:588	meso-diaminopimelic acid	565:588	meso-diaminopimelic acid	565:588	Analysis of the cell wall revealed the presence of meso-diaminopimelic acid and N-acetylmuramic acid in the peptidoglycan.
29543147	5	3	attach	presence	553:560	arg1	peptidoglycan					622:634	the peptidoglycan	618:634	the peptidoglycan	618:634	Analysis of the cell wall revealed the presence of meso-diaminopimelic acid and N-acetylmuramic acid in the peptidoglycan.
29543147	5	3	attach	presence	553:560	arg2	acid					610:613	N-acetylmuramic acid	594:613	N-acetylmuramic acid	594:613	Analysis of the cell wall revealed the presence of meso-diaminopimelic acid and N-acetylmuramic acid in the peptidoglycan.
29543147	3	4	theme	Khitchakut	271:280	arg1	district					282:289	Khao Khitchakut district	266:289	Khao Khitchakut district	266:289	at Khao Khitchakut district, Chantaburi province, Thailand, was characterized by using a polyphasic approach.
29543147	4	5	theme	flexuous	469:476	arg1	chains					478:483	flexuous chains	469:483	flexuous chains of smooth-ornamented spores	469:511	The strain formed a branched substrate and aerial mycelia which differentiated into straight to flexuous chains of smooth-ornamented spores.
29543147	4	6	theme	branched	393:400	arg1	substrate					402:410	a branched substrate	391:410	a branched substrate	391:410	The strain formed a branched substrate and aerial mycelia which differentiated into straight to flexuous chains of smooth-ornamented spores.
29543147	15	7	theme	=TBRC	1663:1667	arg1	113074T					1680:1686	=TBRC 7225T=NBRC 113074T	1663:1686	=TBRC 7225T=NBRC 113074T	1663:1686	nov. (=TBRC 7225T=NBRC 113074T) is proposed.
29543147	15	7	theme	=TBRC	1663:1667	arg1	nov					1657:1659	nov	1657:1659	nov	1657:1659	nov. (=TBRC 7225T=NBRC 113074T) is proposed.
29543147	2	8	theme	Gaertn	254:259	arg1	roots					196:200	the roots	192:200	the roots of an Indian oak tree [Barringtonia acutangula (L.) Gaertn	192:259	A novel actinomycete strain, designated GKU 128T, isolated from the roots of an Indian oak tree [Barringtonia acutangula (L.) Gaertn.]
29543147	10	9	dep	C16 	975:978	arg1	acid					1035:1038	tuberculostearic acid	1018:1038	tuberculostearic acid	1018:1038	The major fatty acids were C16 : 0, C18 : 1ω9c and 10-methyl C18 : 0 (tuberculostearic acid).
29543147	10	9	dep	C16 	975:978	arg1	 0					1014:1015	 0	1014:1015	C16 : 0, C18 : 1ω9c and 10-methyl C18 : 0 (tuberculostearic acid)	975:1039	The major fatty acids were C16 : 0, C18 : 1ω9c and 10-methyl C18 : 0 (tuberculostearic acid).
29543147	10	9	dep	C16 	975:978	arg1	 1ω9c					989:993	 1ω9c	989:993	 1ω9c	989:993	The major fatty acids were C16 : 0, C18 : 1ω9c and 10-methyl C18 : 0 (tuberculostearic acid).
29543147	10	9	dep	C16 	975:978	arg1	C18 					1009:1012	10-methyl C18 	999:1012	10-methyl C18 	999:1012	The major fatty acids were C16 : 0, C18 : 1ω9c and 10-methyl C18 : 0 (tuberculostearic acid).
29543147	10	9	dep	C16 	975:978	arg1	C18 					984:987	C18 	984:987	C18 	984:987	The major fatty acids were C16 : 0, C18 : 1ω9c and 10-methyl C18 : 0 (tuberculostearic acid).
29543147	15	10	theme	7225T=NBRC	1669:1678	arg1	113074T					1680:1686	=TBRC 7225T=NBRC 113074T	1663:1686	=TBRC 7225T=NBRC 113074T	1663:1686	nov. (=TBRC 7225T=NBRC 113074T) is proposed.
29543147	15	10	theme	7225T=NBRC	1669:1678	arg1	nov					1657:1659	nov	1657:1659	nov	1657:1659	nov. (=TBRC 7225T=NBRC 113074T) is proposed.
29543147	2	11	theme	actinomycete	136:147	arg1	strain					149:154	A novel actinomycete strain	128:154	A novel actinomycete strain	128:154	A novel actinomycete strain, designated GKU 128T, isolated from the roots of an Indian oak tree [Barringtonia acutangula (L.) Gaertn.]
29543147	12	12	theme	JCM	1278:1280	arg1	9371T					1282:1286	fibrosa JCM 9371T	1270:1286	fibrosa JCM 9371T (98.7 %)	1270:1295	Based on 16S rRNA gene sequence analysis, strain GKU 128T was closely related to the type strains of Actinomadura nitritigenes NBRC 15918T (99.2 % sequence similarity) and Actinomadura fibrosa JCM 9371T (98.7 %).
29543147	12	12	theme	JCM	1278:1280	arg1	%					1294:1294	98.7 %	1289:1294	98.7 %	1289:1294	Based on 16S rRNA gene sequence analysis, strain GKU 128T was closely related to the type strains of Actinomadura nitritigenes NBRC 15918T (99.2 % sequence similarity) and Actinomadura fibrosa JCM 9371T (98.7 %).
29543147	5	13	theme	meso-diaminopimelic	565:583	arg1	acid					585:588	meso-diaminopimelic acid	565:588	meso-diaminopimelic acid	565:588	Analysis of the cell wall revealed the presence of meso-diaminopimelic acid and N-acetylmuramic acid in the peptidoglycan.
29543147	2	14	theme	L.	250:251	arg1	Gaertn					254:259	an Indian oak tree [Barringtonia acutangula (L.) Gaertn	205:259	an Indian oak tree [Barringtonia acutangula (L.) Gaertn	205:259	A novel actinomycete strain, designated GKU 128T, isolated from the roots of an Indian oak tree [Barringtonia acutangula (L.) Gaertn.]
29543147	13	15	theme	relatedness	1320:1330	arg1	%					1411:1411	less than 19 %	1398:1411	less than 19 %	1398:1411	The levels of DNA-DNA relatedness between strain GKU 128T and the closely related type species were less than 19 %.
29543147	13	15	theme	relatedness	1320:1330	arg1	levels					1302:1307	The levels	1298:1307	The levels of DNA-DNA relatedness between strain GKU 128T and the closely related type species	1298:1391	The levels of DNA-DNA relatedness between strain GKU 128T and the closely related type species were less than 19 %.
29543147	12	16	theme	type	1170:1173	arg1	strains					1175:1181	the type strains	1166:1181	the type strains of Actinomadura nitritigenes NBRC 15918T (99.2 % sequence similarity) and Actinomadura fibrosa JCM 9371T (98.7 %)	1166:1295	Based on 16S rRNA gene sequence analysis, strain GKU 128T was closely related to the type strains of Actinomadura nitritigenes NBRC 15918T (99.2 % sequence similarity) and Actinomadura fibrosa JCM 9371T (98.7 %).
29543147	2	17	theme	novel	130:134	arg1	strain					149:154	A novel actinomycete strain	128:154	A novel actinomycete strain	128:154	A novel actinomycete strain, designated GKU 128T, isolated from the roots of an Indian oak tree [Barringtonia acutangula (L.) Gaertn.]
29543147	5	18	theme	acid	585:588	arg1	presence					553:560	the presence	549:560	the presence of meso-diaminopimelic acid and N-acetylmuramic acid in the peptidoglycan	549:634	Analysis of the cell wall revealed the presence of meso-diaminopimelic acid and N-acetylmuramic acid in the peptidoglycan.
29543147	12	19	dep	15918T	1217:1222	arg1	9371T					1282:1286	fibrosa JCM 9371T	1270:1286	fibrosa JCM 9371T (98.7 %)	1270:1295	Based on 16S rRNA gene sequence analysis, strain GKU 128T was closely related to the type strains of Actinomadura nitritigenes NBRC 15918T (99.2 % sequence similarity) and Actinomadura fibrosa JCM 9371T (98.7 %).
29543147	12	19	dep	15918T	1217:1222	arg1	%					1294:1294	98.7 %	1289:1294	98.7 %	1289:1294	Based on 16S rRNA gene sequence analysis, strain GKU 128T was closely related to the type strains of Actinomadura nitritigenes NBRC 15918T (99.2 % sequence similarity) and Actinomadura fibrosa JCM 9371T (98.7 %).
29543147	2	20	attach	isolated	178:185	arg2	strain					149:154	A novel actinomycete strain	128:154	A novel actinomycete strain	128:154	A novel actinomycete strain, designated GKU 128T, isolated from the roots of an Indian oak tree [Barringtonia acutangula (L.) Gaertn.]
29543147	2	20	attach	isolated	178:185	arg1	roots					196:200	the roots	192:200	the roots of an Indian oak tree [Barringtonia acutangula (L.) Gaertn	192:259	A novel actinomycete strain, designated GKU 128T, isolated from the roots of an Indian oak tree [Barringtonia acutangula (L.) Gaertn.]
29543147	14	21	theme	novel	1566:1570	arg1	species					1572:1578	a novel species	1564:1578	a novel species	1564:1578	On the basis of phenotypic and genotypic characteristics, strain GKU 128T could be distinguished from its closely related type strains and represents a novel species of the genus Actinomadura, for which the name Actinomadura barringtoniae sp.
29543147	14	22	theme	strain	1472:1477	arg1	128T					1483:1486	strain GKU 128T	1472:1486	strain GKU 128T	1472:1486	On the basis of phenotypic and genotypic characteristics, strain GKU 128T could be distinguished from its closely related type strains and represents a novel species of the genus Actinomadura, for which the name Actinomadura barringtoniae sp.
29543147	5	23	theme	N-acetylmuramic	594:608	arg1	acid					610:613	N-acetylmuramic acid	594:613	N-acetylmuramic acid	594:613	Analysis of the cell wall revealed the presence of meso-diaminopimelic acid and N-acetylmuramic acid in the peptidoglycan.
29543147	5	24	theme	cell	530:533	arg1	wall					535:538	the cell wall	526:538	the cell wall	526:538	Analysis of the cell wall revealed the presence of meso-diaminopimelic acid and N-acetylmuramic acid in the peptidoglycan.
29543147	2	25	theme	acutangula	238:247	arg1	Gaertn					254:259	an Indian oak tree [Barringtonia acutangula (L.) Gaertn	205:259	an Indian oak tree [Barringtonia acutangula (L.) Gaertn	205:259	A novel actinomycete strain, designated GKU 128T, isolated from the roots of an Indian oak tree [Barringtonia acutangula (L.) Gaertn.]
29543147	12	26	theme	strain	1127:1132	arg1	128T					1138:1141	strain GKU 128T	1127:1141	strain GKU 128T	1127:1141	Based on 16S rRNA gene sequence analysis, strain GKU 128T was closely related to the type strains of Actinomadura nitritigenes NBRC 15918T (99.2 % sequence similarity) and Actinomadura fibrosa JCM 9371T (98.7 %).
29543147	11	27	theme	DNA	1054:1056	arg1	%					1082:1082	70.5 mol%	1074:1082	70.5 mol%	1074:1082	The genomic DNA G+C content was 70.5 mol%.
29543147	11	27	theme	DNA	1054:1056	arg1	G+C content					1058:1068	The genomic DNA G+C content	1042:1068	The genomic DNA G+C content	1042:1068	The genomic DNA G+C content was 70.5 mol%.
29543147	0	28	theme	barringtoniae	13:25	arg1	sp					27:28	Actinomadura barringtoniae sp	0:28	Actinomadura barringtoniae sp.	0:29	Actinomadura barringtoniae sp.
29543147	12	29	theme	16S	1094:1096	arg1	analysis					1117:1124	16S rRNA gene sequence analysis	1094:1124	16S rRNA gene sequence analysis	1094:1124	Based on 16S rRNA gene sequence analysis, strain GKU 128T was closely related to the type strains of Actinomadura nitritigenes NBRC 15918T (99.2 % sequence similarity) and Actinomadura fibrosa JCM 9371T (98.7 %).
29543147	5	30	theme	acid	610:613	arg1	presence					553:560	the presence	549:560	the presence of meso-diaminopimelic acid and N-acetylmuramic acid in the peptidoglycan	549:634	Analysis of the cell wall revealed the presence of meso-diaminopimelic acid and N-acetylmuramic acid in the peptidoglycan.
29543147	5	31	theme	wall	535:538	arg1	Analysis					514:521	Analysis	514:521	Analysis of the cell wall	514:538	Analysis of the cell wall revealed the presence of meso-diaminopimelic acid and N-acetylmuramic acid in the peptidoglycan.
29543147	4	32	theme	aerial	416:421	arg1	mycelia					423:429	aerial mycelia	416:429	aerial mycelia	416:429	The strain formed a branched substrate and aerial mycelia which differentiated into straight to flexuous chains of smooth-ornamented spores.
29543147	0	33	theme	Actinomadura	0:11	arg1	sp					27:28	Actinomadura barringtoniae sp	0:28	Actinomadura barringtoniae sp.	0:29	Actinomadura barringtoniae sp.
29543147	1	34	attach	isolated	64:71	arg2	actinomycete					51:62	an endophytic actinomycete	37:62	an endophytic actinomycete isolated from the roots of Barringtonia acutangula (L.) Gaertn	37:125	nov., an endophytic actinomycete isolated from the roots of Barringtonia acutangula (L.) Gaertn.
29543147	1	34	attach	isolated	64:71	arg1	roots					82:86	the roots	78:86	the roots of Barringtonia acutangula (L.) Gaertn	78:125	nov., an endophytic actinomycete isolated from the roots of Barringtonia acutangula (L.) Gaertn.
29543147	9	35	theme	predominant	875:885	arg1	MK-9					905:908	MK-9	905:908	MK-9	905:908	The predominant menaquinones were MK-9(H6), MK-9(H8), MK-9(H0) and MK-9(H4).
29543147	9	35	theme	predominant	875:885	arg1	menaquinones					887:898	The predominant menaquinones	871:898	The predominant menaquinones	871:898	The predominant menaquinones were MK-9(H6), MK-9(H8), MK-9(H0) and MK-9(H4).
29543147	13	36	theme	type	1380:1383	arg1	species					1385:1391	the closely related type species	1360:1391	the closely related type species	1360:1391	The levels of DNA-DNA relatedness between strain GKU 128T and the closely related type species were less than 19 %.
29543147	13	37	theme	related	1372:1378	arg1	species					1385:1391	the closely related type species	1360:1391	the closely related type species	1360:1391	The levels of DNA-DNA relatedness between strain GKU 128T and the closely related type species were less than 19 %.
29543147	14	38	theme	barringtoniae	1639:1651	arg1	sp					1653:1654	the name Actinomadura barringtoniae sp	1617:1654	the name Actinomadura barringtoniae sp	1617:1654	On the basis of phenotypic and genotypic characteristics, strain GKU 128T could be distinguished from its closely related type strains and represents a novel species of the genus Actinomadura, for which the name Actinomadura barringtoniae sp.
29543147	12	39	theme	15918T	1217:1222	arg1	strains					1175:1181	the type strains	1166:1181	the type strains of Actinomadura nitritigenes NBRC 15918T (99.2 % sequence similarity) and Actinomadura fibrosa JCM 9371T (98.7 %)	1166:1295	Based on 16S rRNA gene sequence analysis, strain GKU 128T was closely related to the type strains of Actinomadura nitritigenes NBRC 15918T (99.2 % sequence similarity) and Actinomadura fibrosa JCM 9371T (98.7 %).
29543147	1	40	theme	Barringtonia	91:102	arg1	Gaertn					120:125	Barringtonia acutangula (L.) Gaertn	91:125	Barringtonia acutangula (L.) Gaertn	91:125	nov., an endophytic actinomycete isolated from the roots of Barringtonia acutangula (L.) Gaertn.
29543147	14	41	theme	genus	1587:1591	arg1	Actinomadura					1593:1604	the genus Actinomadura	1583:1604	the genus Actinomadura	1583:1604	On the basis of phenotypic and genotypic characteristics, strain GKU 128T could be distinguished from its closely related type strains and represents a novel species of the genus Actinomadura, for which the name Actinomadura barringtoniae sp.
29543147	2	42	theme	GKU	168:170	arg1	128T					172:175	GKU 128T	168:175	GKU 128T	168:175	A novel actinomycete strain, designated GKU 128T, isolated from the roots of an Indian oak tree [Barringtonia acutangula (L.) Gaertn.]
29543147	1	43	theme	acutangula	104:113	arg1	Gaertn					120:125	Barringtonia acutangula (L.) Gaertn	91:125	Barringtonia acutangula (L.) Gaertn	91:125	nov., an endophytic actinomycete isolated from the roots of Barringtonia acutangula (L.) Gaertn.
29543147	14	44	theme	related	1528:1534	arg1	strains					1541:1547	its closely related type strains	1516:1547	its closely related type strains	1516:1547	On the basis of phenotypic and genotypic characteristics, strain GKU 128T could be distinguished from its closely related type strains and represents a novel species of the genus Actinomadura, for which the name Actinomadura barringtoniae sp.
29543147	13	45	theme	strain	1340:1345	arg1	128T					1351:1354	strain GKU 128T	1340:1354	strain GKU 128T	1340:1354	The levels of DNA-DNA relatedness between strain GKU 128T and the closely related type species were less than 19 %.
29543147	3	46	theme	polyphasic	352:361	arg1	approach					363:370	a polyphasic approach	350:370	a polyphasic approach	350:370	at Khao Khitchakut district, Chantaburi province, Thailand, was characterized by using a polyphasic approach.
29543147	10	47	theme	tuberculostearic	1018:1033	arg1	 0					1014:1015	 0	1014:1015	C16 : 0, C18 : 1ω9c and 10-methyl C18 : 0 (tuberculostearic acid)	975:1039	The major fatty acids were C16 : 0, C18 : 1ω9c and 10-methyl C18 : 0 (tuberculostearic acid).
29543147	10	47	theme	tuberculostearic	1018:1033	arg1	acid					1035:1038	tuberculostearic acid	1018:1038	tuberculostearic acid	1018:1038	The major fatty acids were C16 : 0, C18 : 1ω9c and 10-methyl C18 : 0 (tuberculostearic acid).
29543147	12	48	theme	%	1230:1230	arg1	15918T					1217:1222	15918T	1217:1222	15918T	1217:1222	Based on 16S rRNA gene sequence analysis, strain GKU 128T was closely related to the type strains of Actinomadura nitritigenes NBRC 15918T (99.2 % sequence similarity) and Actinomadura fibrosa JCM 9371T (98.7 %).
29543147	12	48	theme	%	1230:1230	arg1	similarity					1241:1250	99.2 % sequence similarity	1225:1250	99.2 % sequence similarity	1225:1250	Based on 16S rRNA gene sequence analysis, strain GKU 128T was closely related to the type strains of Actinomadura nitritigenes NBRC 15918T (99.2 % sequence similarity) and Actinomadura fibrosa JCM 9371T (98.7 %).
29543147	6	49	theme	whole-cell	641:650	arg1	glucose					664:670	glucose	664:670	glucose	664:670	The whole-cell sugars were glucose, madurose, mannose, rhamnose and ribose.
29543147	6	49	theme	whole-cell	641:650	arg1	sugars					652:657	The whole-cell sugars	637:657	The whole-cell sugars	637:657	The whole-cell sugars were glucose, madurose, mannose, rhamnose and ribose.
29543147	13	50	theme	GKU	1347:1349	arg1	128T					1351:1354	strain GKU 128T	1340:1354	strain GKU 128T	1340:1354	The levels of DNA-DNA relatedness between strain GKU 128T and the closely related type species were less than 19 %.
29543147	14	51	theme	GKU	1479:1481	arg1	128T					1483:1486	strain GKU 128T	1472:1486	strain GKU 128T	1472:1486	On the basis of phenotypic and genotypic characteristics, strain GKU 128T could be distinguished from its closely related type strains and represents a novel species of the genus Actinomadura, for which the name Actinomadura barringtoniae sp.
29543147	14	52	theme	Actinomadura	1626:1637	arg1	sp					1653:1654	the name Actinomadura barringtoniae sp	1617:1654	the name Actinomadura barringtoniae sp	1617:1654	On the basis of phenotypic and genotypic characteristics, strain GKU 128T could be distinguished from its closely related type strains and represents a novel species of the genus Actinomadura, for which the name Actinomadura barringtoniae sp.
29543147	14	53	theme	Actinomadura	1593:1604	arg1	species					1572:1578	a novel species	1564:1578	a novel species	1564:1578	On the basis of phenotypic and genotypic characteristics, strain GKU 128T could be distinguished from its closely related type strains and represents a novel species of the genus Actinomadura, for which the name Actinomadura barringtoniae sp.
29543147	4	54	theme	smooth-ornamented	488:504	arg1	spores					506:511	smooth-ornamented spores	488:511	smooth-ornamented spores	488:511	The strain formed a branched substrate and aerial mycelia which differentiated into straight to flexuous chains of smooth-ornamented spores.
29543147	14	55	theme	name	1621:1624	arg1	sp					1653:1654	the name Actinomadura barringtoniae sp	1617:1654	the name Actinomadura barringtoniae sp	1617:1654	On the basis of phenotypic and genotypic characteristics, strain GKU 128T could be distinguished from its closely related type strains and represents a novel species of the genus Actinomadura, for which the name Actinomadura barringtoniae sp.
29543147	10	56	theme	fatty	958:962	arg1	C16 					975:978	C16 	975:978	C16 : 0, C18 : 1ω9c and 10-methyl C18 : 0 (tuberculostearic acid)	975:1039	The major fatty acids were C16 : 0, C18 : 1ω9c and 10-methyl C18 : 0 (tuberculostearic acid).
29543147	10	56	theme	fatty	958:962	arg1	acids					964:968	The major fatty acids	948:968	The major fatty acids	948:968	The major fatty acids were C16 : 0, C18 : 1ω9c and 10-methyl C18 : 0 (tuberculostearic acid).
29543147	14	57	theme	genotypic	1445:1453	arg1	characteristics					1455:1469	phenotypic and genotypic characteristics	1430:1469	phenotypic and genotypic characteristics	1430:1469	On the basis of phenotypic and genotypic characteristics, strain GKU 128T could be distinguished from its closely related type strains and represents a novel species of the genus Actinomadura, for which the name Actinomadura barringtoniae sp.
29543147	12	58	theme	GKU	1134:1136	arg1	128T					1138:1141	strain GKU 128T	1127:1141	strain GKU 128T	1127:1141	Based on 16S rRNA gene sequence analysis, strain GKU 128T was closely related to the type strains of Actinomadura nitritigenes NBRC 15918T (99.2 % sequence similarity) and Actinomadura fibrosa JCM 9371T (98.7 %).
29543147	4	59	theme	spores	506:511	arg1	chains					478:483	flexuous chains	469:483	flexuous chains of smooth-ornamented spores	469:511	The strain formed a branched substrate and aerial mycelia which differentiated into straight to flexuous chains of smooth-ornamented spores.
29543147	14	60	theme	type	1536:1539	arg1	strains					1541:1547	its closely related type strains	1516:1547	its closely related type strains	1516:1547	On the basis of phenotypic and genotypic characteristics, strain GKU 128T could be distinguished from its closely related type strains and represents a novel species of the genus Actinomadura, for which the name Actinomadura barringtoniae sp.
29543147	12	61	theme	sequence	1232:1239	arg1	15918T					1217:1222	15918T	1217:1222	15918T	1217:1222	Based on 16S rRNA gene sequence analysis, strain GKU 128T was closely related to the type strains of Actinomadura nitritigenes NBRC 15918T (99.2 % sequence similarity) and Actinomadura fibrosa JCM 9371T (98.7 %).
29543147	12	61	theme	sequence	1232:1239	arg1	similarity					1241:1250	99.2 % sequence similarity	1225:1250	99.2 % sequence similarity	1225:1250	Based on 16S rRNA gene sequence analysis, strain GKU 128T was closely related to the type strains of Actinomadura nitritigenes NBRC 15918T (99.2 % sequence similarity) and Actinomadura fibrosa JCM 9371T (98.7 %).
29543147	1	62	theme	L.	116:117	arg1	Gaertn					120:125	Barringtonia acutangula (L.) Gaertn	91:125	Barringtonia acutangula (L.) Gaertn	91:125	nov., an endophytic actinomycete isolated from the roots of Barringtonia acutangula (L.) Gaertn.
29543147	1	63	theme	endophytic	40:49	arg1	actinomycete					51:62	an endophytic actinomycete	37:62	an endophytic actinomycete isolated from the roots of Barringtonia acutangula (L.) Gaertn	37:125	nov., an endophytic actinomycete isolated from the roots of Barringtonia acutangula (L.) Gaertn.
29543147	10	64	theme	major	952:956	arg1	C16 					975:978	C16 	975:978	C16 : 0, C18 : 1ω9c and 10-methyl C18 : 0 (tuberculostearic acid)	975:1039	The major fatty acids were C16 : 0, C18 : 1ω9c and 10-methyl C18 : 0 (tuberculostearic acid).
29543147	10	64	theme	major	952:956	arg1	acids					964:968	The major fatty acids	948:968	The major fatty acids	948:968	The major fatty acids were C16 : 0, C18 : 1ω9c and 10-methyl C18 : 0 (tuberculostearic acid).
29543147	2	65	theme	[Barringtonia	224:236	arg1	Gaertn					254:259	an Indian oak tree [Barringtonia acutangula (L.) Gaertn	205:259	an Indian oak tree [Barringtonia acutangula (L.) Gaertn	205:259	A novel actinomycete strain, designated GKU 128T, isolated from the roots of an Indian oak tree [Barringtonia acutangula (L.) Gaertn.]
29543147	14	66	theme	phenotypic	1430:1439	arg1	characteristics					1455:1469	phenotypic and genotypic characteristics	1430:1469	phenotypic and genotypic characteristics	1430:1469	On the basis of phenotypic and genotypic characteristics, strain GKU 128T could be distinguished from its closely related type strains and represents a novel species of the genus Actinomadura, for which the name Actinomadura barringtoniae sp.
29543147	1	67	dep	actinomycete	51:62	arg1	nov.					31:34	nov.	31:34	nov.	31:34	nov., an endophytic actinomycete isolated from the roots of Barringtonia acutangula (L.) Gaertn.
29543147	12	68	theme	fibrosa	1270:1276	arg1	9371T					1282:1286	fibrosa JCM 9371T	1270:1286	fibrosa JCM 9371T (98.7 %)	1270:1295	Based on 16S rRNA gene sequence analysis, strain GKU 128T was closely related to the type strains of Actinomadura nitritigenes NBRC 15918T (99.2 % sequence similarity) and Actinomadura fibrosa JCM 9371T (98.7 %).
29543147	12	68	theme	fibrosa	1270:1276	arg1	%					1294:1294	98.7 %	1289:1294	98.7 %	1289:1294	Based on 16S rRNA gene sequence analysis, strain GKU 128T was closely related to the type strains of Actinomadura nitritigenes NBRC 15918T (99.2 % sequence similarity) and Actinomadura fibrosa JCM 9371T (98.7 %).
29543147	12	69	theme	Actinomadura	1257:1268	arg1	strains					1175:1181	the type strains	1166:1181	the type strains of Actinomadura nitritigenes NBRC 15918T (99.2 % sequence similarity) and Actinomadura fibrosa JCM 9371T (98.7 %)	1166:1295	Based on 16S rRNA gene sequence analysis, strain GKU 128T was closely related to the type strains of Actinomadura nitritigenes NBRC 15918T (99.2 % sequence similarity) and Actinomadura fibrosa JCM 9371T (98.7 %).
29543147	11	70	theme	70.5 mol	1074:1081	arg1	%					1082:1082	70.5 mol%	1074:1082	70.5 mol%	1074:1082	The genomic DNA G+C content was 70.5 mol%.
29543147	11	70	theme	70.5 mol	1074:1081	arg1	G+C content					1058:1068	The genomic DNA G+C content	1042:1068	The genomic DNA G+C content	1042:1068	The genomic DNA G+C content was 70.5 mol%.
29543147	2	71	theme	tree	219:222	arg1	Gaertn					254:259	an Indian oak tree [Barringtonia acutangula (L.) Gaertn	205:259	an Indian oak tree [Barringtonia acutangula (L.) Gaertn	205:259	A novel actinomycete strain, designated GKU 128T, isolated from the roots of an Indian oak tree [Barringtonia acutangula (L.) Gaertn.]
29543147	1	72	theme	Gaertn	120:125	arg1	roots					82:86	the roots	78:86	the roots of Barringtonia acutangula (L.) Gaertn	78:125	nov., an endophytic actinomycete isolated from the roots of Barringtonia acutangula (L.) Gaertn.
29543147	13	73	theme	DNA-DNA	1312:1318	arg1	relatedness					1320:1330	DNA-DNA relatedness	1312:1330	DNA-DNA relatedness between strain GKU 128T and the closely related type species	1312:1391	The levels of DNA-DNA relatedness between strain GKU 128T and the closely related type species were less than 19 %.
29543147	2	74	theme	oak	215:217	arg1	Gaertn					254:259	an Indian oak tree [Barringtonia acutangula (L.) Gaertn	205:259	an Indian oak tree [Barringtonia acutangula (L.) Gaertn	205:259	A novel actinomycete strain, designated GKU 128T, isolated from the roots of an Indian oak tree [Barringtonia acutangula (L.) Gaertn.]
29543147	12	75	theme	sequence	1108:1115	arg1	analysis					1117:1124	16S rRNA gene sequence analysis	1094:1124	16S rRNA gene sequence analysis	1094:1124	Based on 16S rRNA gene sequence analysis, strain GKU 128T was closely related to the type strains of Actinomadura nitritigenes NBRC 15918T (99.2 % sequence similarity) and Actinomadura fibrosa JCM 9371T (98.7 %).
29543147	11	76	theme	genomic	1046:1052	arg1	%					1082:1082	70.5 mol%	1074:1082	70.5 mol%	1074:1082	The genomic DNA G+C content was 70.5 mol%.
29543147	11	76	theme	genomic	1046:1052	arg1	G+C content					1058:1068	The genomic DNA G+C content	1042:1068	The genomic DNA G+C content	1042:1068	The genomic DNA G+C content was 70.5 mol%.
29543147	8	77	theme	major	744:748	arg1	diphosphatidylglycerol					769:790	diphosphatidylglycerol	769:790	diphosphatidylglycerol	769:790	The major phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositolmannoside.
29543147	8	77	theme	major	744:748	arg1	phospholipids					750:762	The major phospholipids	740:762	The major phospholipids	740:762	The major phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositolmannoside.
29543147	10	78	theme	10-methyl	999:1007	arg1	C18 					1009:1012	10-methyl C18 	999:1012	10-methyl C18 	999:1012	The major fatty acids were C16 : 0, C18 : 1ω9c and 10-methyl C18 : 0 (tuberculostearic acid).
29543147	2	79	theme	Indian	208:213	arg1	Gaertn					254:259	an Indian oak tree [Barringtonia acutangula (L.) Gaertn	205:259	an Indian oak tree [Barringtonia acutangula (L.) Gaertn	205:259	A novel actinomycete strain, designated GKU 128T, isolated from the roots of an Indian oak tree [Barringtonia acutangula (L.) Gaertn.]
29543147	14	80	dep	characteristics	1455:1469	arg1	basis					1421:1425	basis	1421:1425	basis	1421:1425	On the basis of phenotypic and genotypic characteristics, strain GKU 128T could be distinguished from its closely related type strains and represents a novel species of the genus Actinomadura, for which the name Actinomadura barringtoniae sp.
29543147	14	80	dep	characteristics	1455:1469	arg1	the					1417:1419	the	1417:1419	the	1417:1419	On the basis of phenotypic and genotypic characteristics, strain GKU 128T could be distinguished from its closely related type strains and represents a novel species of the genus Actinomadura, for which the name Actinomadura barringtoniae sp.
29543147	12	81	theme	gene	1103:1106	arg1	analysis					1117:1124	16S rRNA gene sequence analysis	1094:1124	16S rRNA gene sequence analysis	1094:1124	Based on 16S rRNA gene sequence analysis, strain GKU 128T was closely related to the type strains of Actinomadura nitritigenes NBRC 15918T (99.2 % sequence similarity) and Actinomadura fibrosa JCM 9371T (98.7 %).
29543147	7	82	theme	Mycolic	713:719	arg1	acids					721:725	Mycolic acids	713:725	Mycolic acids	713:725	Mycolic acids were absent.
29395538	6	0	theme	%	944:944	arg1	NaCl					952:955	0-8% (w/v) NaCl	941:955	0-8% (w/v) NaCl (optimum 1%)	941:968	Strains 0911MAR22V3T and 0911TES10J4 grew at 10-47°C (optimum 37°C), at pH 6-9.5 and with 0-8% (w/v) NaCl (optimum 1%).
29395538	6	0	theme	%	944:944	arg1	%					967:967	optimum 1%	958:967	optimum 1%	958:967	Strains 0911MAR22V3T and 0911TES10J4 grew at 10-47°C (optimum 37°C), at pH 6-9.5 and with 0-8% (w/v) NaCl (optimum 1%).
29395538	8	1	theme	onubensis	1491:1499	arg1	sp					1501:1502	name Bacillus onubensis sp	1477:1502	the name Bacillus onubensis sp	1473:1502	On the basis of their phylogenetic relatedness and their phenotypic and genotypic features, the strains 0911MAR22V3T and 0911TES10J4 should be attributed to a novel species within the genus Bacillus, for which the name Bacillus onubensis sp.
29395538	4	2	theme	0911MAR22V3T	541:552	arg1	values					520:525	DNA-DNA hybridization values	498:525	DNA-DNA hybridization values of the strain 0911MAR22V3T with respect to strain 0911TES10J4 and B. humi DSM 16318T	498:610	DNA-DNA hybridization values of the strain 0911MAR22V3T with respect to strain 0911TES10J4 and B. humi DSM 16318T were 76.8% (73.9%, reciprocal) and 56.9% (63.3%, reciprocal analysis), respectively.
29395538	8	3	theme	genotypic	1335:1343	arg1	features					1345:1352	their phenotypic and genotypic features	1314:1352	their phenotypic and genotypic features	1314:1352	On the basis of their phylogenetic relatedness and their phenotypic and genotypic features, the strains 0911MAR22V3T and 0911TES10J4 should be attributed to a novel species within the genus Bacillus, for which the name Bacillus onubensis sp.
29395538	2	4	dep	two	268:270	arg1	show					272:275	show	272:275	show caves, located in Andalusia, Southern Spain	272:319	Two Gram-positive, catalase-positive, oxidase-negative, motile, endospore-forming, rod-shaped bacteria, designated as 0911MAR22V3T and 0911TES10J4, were isolated from air samples collected in two show caves, located in Andalusia, Southern Spain.
29395538	7	5	from	%	1260:1260	arg1	strains					979:985	both strains	974:985	both strains	974:985	In both strains the dominant isoprenoid quinone was MK-7, the major cellular polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and two more phospholipids, the predominant fatty acids were iso-C15:0 and anteiso-C15:0 and the DNA G+C content was 38mol%.
29395538	4	6	theme	strain	534:539	arg1	0911MAR22V3T					541:552	the strain 0911MAR22V3T	530:552	the strain 0911MAR22V3T with respect to strain 0911TES10J4 and B. humi DSM 16318T	530:610	DNA-DNA hybridization values of the strain 0911MAR22V3T with respect to strain 0911TES10J4 and B. humi DSM 16318T were 76.8% (73.9%, reciprocal) and 56.9% (63.3%, reciprocal analysis), respectively.
29395538	2	7	theme	Gram-positive	80:92	arg1	bacteria					170:177	Two Gram-positive, catalase-positive, oxidase-negative, motile, endospore-forming, rod-shaped bacteria	76:177	Two Gram-positive, catalase-positive, oxidase-negative, motile, endospore-forming, rod-shaped bacteria	76:177	Two Gram-positive, catalase-positive, oxidase-negative, motile, endospore-forming, rod-shaped bacteria, designated as 0911MAR22V3T and 0911TES10J4, were isolated from air samples collected in two show caves, located in Andalusia, Southern Spain.
29395538	10	8	theme	type	1527:1530	arg1	strain					1532:1537	The type strain	1523:1537	The type strain	1523:1537	The type strain is 0911MAR22V3T (=LMG 27963T=CECT 8479T); and strain 0911TES10J4 (CECT 8478) is a reference strain.
29395538	10	8	theme	type	1527:1530	arg1	0911MAR22V3T					1542:1553	0911MAR22V3T	1542:1553	0911MAR22V3T (=LMG 27963T=CECT 8479T)	1542:1578	The type strain is 0911MAR22V3T (=LMG 27963T=CECT 8479T); and strain 0911TES10J4 (CECT 8478) is a reference strain.
29395538	2	9	from	Andalusia	295:303	arg1	located					284:290	located	284:290	located	284:290	Two Gram-positive, catalase-positive, oxidase-negative, motile, endospore-forming, rod-shaped bacteria, designated as 0911MAR22V3T and 0911TES10J4, were isolated from air samples collected in two show caves, located in Andalusia, Southern Spain.
29395538	2	10	from	located	284:290	arg1	Andalusia					295:303	Andalusia	295:303	Andalusia	295:303	Two Gram-positive, catalase-positive, oxidase-negative, motile, endospore-forming, rod-shaped bacteria, designated as 0911MAR22V3T and 0911TES10J4, were isolated from air samples collected in two show caves, located in Andalusia, Southern Spain.
29395538	2	10	from	located	284:290	arg1	Spain					315:319	Southern Spain	306:319	Southern Spain	306:319	Two Gram-positive, catalase-positive, oxidase-negative, motile, endospore-forming, rod-shaped bacteria, designated as 0911MAR22V3T and 0911TES10J4, were isolated from air samples collected in two show caves, located in Andalusia, Southern Spain.
29395538	4	11	dep	%	651:651	arg1	%					658:658	63.3%	654:658	63.3%	654:658	DNA-DNA hybridization values of the strain 0911MAR22V3T with respect to strain 0911TES10J4 and B. humi DSM 16318T were 76.8% (73.9%, reciprocal) and 56.9% (63.3%, reciprocal analysis), respectively.
29395538	4	11	dep	%	651:651	arg1	analysis					672:679	reciprocal analysis	661:679	reciprocal analysis	661:679	DNA-DNA hybridization values of the strain 0911MAR22V3T with respect to strain 0911TES10J4 and B. humi DSM 16318T were 76.8% (73.9%, reciprocal) and 56.9% (63.3%, reciprocal analysis), respectively.
29395538	5	12	theme	nucleotide	718:727	arg1	ANI					739:741	ANI	739:741	ANI	739:741	Whole genome average nucleotide identity (ANI) values of both strains were in the threshold value for species delineation and less than 85% with B. humi.
29395538	5	12	theme	nucleotide	718:727	arg1	identity					729:736	Whole genome average nucleotide identity	697:736	Whole genome average nucleotide identity (ANI) values of both strains	697:765	Whole genome average nucleotide identity (ANI) values of both strains were in the threshold value for species delineation and less than 85% with B. humi.
29395538	10	13	theme	27963T=CECT	1561:1571	arg1	8479T					1573:1577	=LMG 27963T=CECT 8479T	1556:1577	=LMG 27963T=CECT 8479T	1556:1577	The type strain is 0911MAR22V3T (=LMG 27963T=CECT 8479T); and strain 0911TES10J4 (CECT 8478) is a reference strain.
29395538	10	13	theme	27963T=CECT	1561:1571	arg1	0911MAR22V3T					1542:1553	0911MAR22V3T	1542:1553	0911MAR22V3T (=LMG 27963T=CECT 8479T)	1542:1578	The type strain is 0911MAR22V3T (=LMG 27963T=CECT 8479T); and strain 0911TES10J4 (CECT 8478) is a reference strain.
29395538	7	14	theme	polar	1048:1052	arg1	lipids					1054:1059	the major cellular polar lipids	1029:1059	the major cellular polar lipids	1029:1059	In both strains the dominant isoprenoid quinone was MK-7, the major cellular polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and two more phospholipids, the predominant fatty acids were iso-C15:0 and anteiso-C15:0 and the DNA G+C content was 38mol%.
29395538	7	14	theme	polar	1048:1052	arg1	diphosphatidylglycerol					1066:1087	diphosphatidylglycerol	1066:1087	diphosphatidylglycerol	1066:1087	In both strains the dominant isoprenoid quinone was MK-7, the major cellular polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and two more phospholipids, the predominant fatty acids were iso-C15:0 and anteiso-C15:0 and the DNA G+C content was 38mol%.
29395538	4	15	theme	reciprocal	661:670	arg1	%					658:658	63.3%	654:658	63.3%	654:658	DNA-DNA hybridization values of the strain 0911MAR22V3T with respect to strain 0911TES10J4 and B. humi DSM 16318T were 76.8% (73.9%, reciprocal) and 56.9% (63.3%, reciprocal analysis), respectively.
29395538	4	15	theme	reciprocal	661:670	arg1	analysis					672:679	reciprocal analysis	661:679	reciprocal analysis	661:679	DNA-DNA hybridization values of the strain 0911MAR22V3T with respect to strain 0911TES10J4 and B. humi DSM 16318T were 76.8% (73.9%, reciprocal) and 56.9% (63.3%, reciprocal analysis), respectively.
29395538	5	16	with	%	835:835	arg1	B.					842:843	B.	842:843	B.	842:843	Whole genome average nucleotide identity (ANI) values of both strains were in the threshold value for species delineation and less than 85% with B. humi.
29395538	7	17	theme	dominant	991:998	arg1	quinone					1011:1017	the dominant isoprenoid quinone	987:1017	the dominant isoprenoid quinone	987:1017	In both strains the dominant isoprenoid quinone was MK-7, the major cellular polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and two more phospholipids, the predominant fatty acids were iso-C15:0 and anteiso-C15:0 and the DNA G+C content was 38mol%.
29395538	7	17	theme	dominant	991:998	arg1	MK-7					1023:1026	MK-7	1023:1026	MK-7	1023:1026	In both strains the dominant isoprenoid quinone was MK-7, the major cellular polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and two more phospholipids, the predominant fatty acids were iso-C15:0 and anteiso-C15:0 and the DNA G+C content was 38mol%.
29395538	5	18	theme	species	799:805	arg1	delineation					807:817	species delineation	799:817	species delineation	799:817	Whole genome average nucleotide identity (ANI) values of both strains were in the threshold value for species delineation and less than 85% with B. humi.
29395538	5	19	theme	average	710:716	arg1	ANI					739:741	ANI	739:741	ANI	739:741	Whole genome average nucleotide identity (ANI) values of both strains were in the threshold value for species delineation and less than 85% with B. humi.
29395538	5	19	theme	average	710:716	arg1	identity					729:736	Whole genome average nucleotide identity	697:736	Whole genome average nucleotide identity (ANI) values of both strains	697:765	Whole genome average nucleotide identity (ANI) values of both strains were in the threshold value for species delineation and less than 85% with B. humi.
29395538	4	20	dep	%	628:628	arg1	reciprocal					631:640	reciprocal	631:640	reciprocal	631:640	DNA-DNA hybridization values of the strain 0911MAR22V3T with respect to strain 0911TES10J4 and B. humi DSM 16318T were 76.8% (73.9%, reciprocal) and 56.9% (63.3%, reciprocal analysis), respectively.
29395538	8	21	theme	Bacillus	1482:1489	arg1	sp					1501:1502	name Bacillus onubensis sp	1477:1502	the name Bacillus onubensis sp	1473:1502	On the basis of their phylogenetic relatedness and their phenotypic and genotypic features, the strains 0911MAR22V3T and 0911TES10J4 should be attributed to a novel species within the genus Bacillus, for which the name Bacillus onubensis sp.
29395538	3	22	theme	Phylogenetic	322:333	arg1	analysis					335:342	Phylogenetic analysis	322:342	Phylogenetic analysis based on 16S rRNA gene sequences	322:375	Phylogenetic analysis based on 16S rRNA gene sequences indicated that both strains were indistinguishable and they were most closely related to Bacillus humi DSM 16318T (98%).
29395538	8	23	dep	genus	1447:1451	arg1	Bacillus					1453:1460	the genus Bacillus	1443:1460	the genus Bacillus	1443:1460	On the basis of their phylogenetic relatedness and their phenotypic and genotypic features, the strains 0911MAR22V3T and 0911TES10J4 should be attributed to a novel species within the genus Bacillus, for which the name Bacillus onubensis sp.
29395538	0	24	theme	Bacillus	0:7	arg1	sp					19:20	Bacillus onubensis sp	0:20	Bacillus onubensis sp.	0:21	Bacillus onubensis sp.
29395538	5	25	theme	strains	759:765	arg1	values					744:749	Whole genome average nucleotide identity (ANI) values	697:749	Whole genome average nucleotide identity (ANI) values of both strains	697:765	Whole genome average nucleotide identity (ANI) values of both strains were in the threshold value for species delineation and less than 85% with B. humi.
29395538	0	26	theme	onubensis	9:17	arg1	sp					19:20	Bacillus onubensis sp	0:20	Bacillus onubensis sp.	0:21	Bacillus onubensis sp.
29395538	2	27	theme	located	284:290	arg1	caves					277:281	caves	277:281	caves	277:281	Two Gram-positive, catalase-positive, oxidase-negative, motile, endospore-forming, rod-shaped bacteria, designated as 0911MAR22V3T and 0911TES10J4, were isolated from air samples collected in two show caves, located in Andalusia, Southern Spain.
29395538	1	28	theme	Andalusian	58:67	arg1	caves					69:73	two Andalusian caves	54:73	two Andalusian caves	54:73	nov., isolated from the air of two Andalusian caves.
29395538	7	29	theme	more	1146:1149	arg1	phospholipids					1151:1163	two more phospholipids	1142:1163	two more phospholipids	1142:1163	In both strains the dominant isoprenoid quinone was MK-7, the major cellular polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and two more phospholipids, the predominant fatty acids were iso-C15:0 and anteiso-C15:0 and the DNA G+C content was 38mol%.
29395538	3	30	theme	gene	362:365	arg1	sequences					367:375	16S rRNA gene sequences	353:375	16S rRNA gene sequences	353:375	Phylogenetic analysis based on 16S rRNA gene sequences indicated that both strains were indistinguishable and they were most closely related to Bacillus humi DSM 16318T (98%).
29395538	5	31	with	delineation	807:817	arg1	B.					842:843	B.	842:843	B.	842:843	Whole genome average nucleotide identity (ANI) values of both strains were in the threshold value for species delineation and less than 85% with B. humi.
29395538	1	32	theme	caves	69:73	arg1	air					47:49	the air	43:49	the air of two Andalusian caves	43:73	nov., isolated from the air of two Andalusian caves.
29395538	5	33	theme	identity	729:736	arg1	values					744:749	Whole genome average nucleotide identity (ANI) values	697:749	Whole genome average nucleotide identity (ANI) values of both strains	697:765	Whole genome average nucleotide identity (ANI) values of both strains were in the threshold value for species delineation and less than 85% with B. humi.
29395538	4	34	theme	hybridization	506:518	arg1	values					520:525	DNA-DNA hybridization values	498:525	DNA-DNA hybridization values of the strain 0911MAR22V3T with respect to strain 0911TES10J4 and B. humi DSM 16318T	498:610	DNA-DNA hybridization values of the strain 0911MAR22V3T with respect to strain 0911TES10J4 and B. humi DSM 16318T were 76.8% (73.9%, reciprocal) and 56.9% (63.3%, reciprocal analysis), respectively.
29395538	4	35	theme	humi	596:599	arg1	16318T					605:610	B. humi DSM 16318T	593:610	B. humi DSM 16318T	593:610	DNA-DNA hybridization values of the strain 0911MAR22V3T with respect to strain 0911TES10J4 and B. humi DSM 16318T were 76.8% (73.9%, reciprocal) and 56.9% (63.3%, reciprocal analysis), respectively.
29395538	3	36	theme	16S	353:355	arg1	sequences					367:375	16S rRNA gene sequences	353:375	16S rRNA gene sequences	353:375	Phylogenetic analysis based on 16S rRNA gene sequences indicated that both strains were indistinguishable and they were most closely related to Bacillus humi DSM 16318T (98%).
29395538	8	37	dep	relatedness	1298:1308	arg1	basis					1270:1274	basis	1270:1274	basis	1270:1274	On the basis of their phylogenetic relatedness and their phenotypic and genotypic features, the strains 0911MAR22V3T and 0911TES10J4 should be attributed to a novel species within the genus Bacillus, for which the name Bacillus onubensis sp.
29395538	8	37	dep	relatedness	1298:1308	arg1	the					1266:1268	the	1266:1268	the	1266:1268	On the basis of their phylogenetic relatedness and their phenotypic and genotypic features, the strains 0911MAR22V3T and 0911TES10J4 should be attributed to a novel species within the genus Bacillus, for which the name Bacillus onubensis sp.
29395538	7	38	theme	DNA	1235:1237	arg1	content					1243:1249	the DNA G+C content	1231:1249	the DNA G+C content	1231:1249	In both strains the dominant isoprenoid quinone was MK-7, the major cellular polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and two more phospholipids, the predominant fatty acids were iso-C15:0 and anteiso-C15:0 and the DNA G+C content was 38mol%.
29395538	7	38	theme	DNA	1235:1237	arg1	%					1260:1260	38mol%	1255:1260	38mol%	1255:1260	In both strains the dominant isoprenoid quinone was MK-7, the major cellular polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and two more phospholipids, the predominant fatty acids were iso-C15:0 and anteiso-C15:0 and the DNA G+C content was 38mol%.
29395538	7	39	theme	G+C	1239:1241	arg1	content					1243:1249	the DNA G+C content	1231:1249	the DNA G+C content	1231:1249	In both strains the dominant isoprenoid quinone was MK-7, the major cellular polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and two more phospholipids, the predominant fatty acids were iso-C15:0 and anteiso-C15:0 and the DNA G+C content was 38mol%.
29395538	7	39	theme	G+C	1239:1241	arg1	%					1260:1260	38mol%	1255:1260	38mol%	1255:1260	In both strains the dominant isoprenoid quinone was MK-7, the major cellular polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and two more phospholipids, the predominant fatty acids were iso-C15:0 and anteiso-C15:0 and the DNA G+C content was 38mol%.
29395538	4	40	theme	B.	593:594	arg1	16318T					605:610	B. humi DSM 16318T	593:610	B. humi DSM 16318T	593:610	DNA-DNA hybridization values of the strain 0911MAR22V3T with respect to strain 0911TES10J4 and B. humi DSM 16318T were 76.8% (73.9%, reciprocal) and 56.9% (63.3%, reciprocal analysis), respectively.
29395538	3	41	theme	humi	475:478	arg1	%					494:494	98%	492:494	98%	492:494	Phylogenetic analysis based on 16S rRNA gene sequences indicated that both strains were indistinguishable and they were most closely related to Bacillus humi DSM 16318T (98%).
29395538	3	41	theme	humi	475:478	arg1	DSM					480:482	Bacillus humi DSM 16318T	466:489	Bacillus humi DSM 16318T (98%)	466:495	Phylogenetic analysis based on 16S rRNA gene sequences indicated that both strains were indistinguishable and they were most closely related to Bacillus humi DSM 16318T (98%).
29395538	6	42	theme	optimum	905:911	arg1	10-47°C					896:902	10-47°C	896:902	10-47°C (optimum 37°C)	896:917	Strains 0911MAR22V3T and 0911TES10J4 grew at 10-47°C (optimum 37°C), at pH 6-9.5 and with 0-8% (w/v) NaCl (optimum 1%).
29395538	6	42	theme	optimum	905:911	arg1	37°C					913:916	optimum 37°C	905:916	optimum 37°C	905:916	Strains 0911MAR22V3T and 0911TES10J4 grew at 10-47°C (optimum 37°C), at pH 6-9.5 and with 0-8% (w/v) NaCl (optimum 1%).
29395538	10	43	theme	strain	1585:1590	arg1	CECT					1605:1608	CECT 8478	1605:1613	CECT 8478	1605:1613	The type strain is 0911MAR22V3T (=LMG 27963T=CECT 8479T); and strain 0911TES10J4 (CECT 8478) is a reference strain.
29395538	10	43	theme	strain	1585:1590	arg1	0911TES10J4					1592:1602	strain 0911TES10J4	1585:1602	strain 0911TES10J4 (CECT 8478)	1585:1614	The type strain is 0911MAR22V3T (=LMG 27963T=CECT 8479T); and strain 0911TES10J4 (CECT 8478) is a reference strain.
29395538	10	43	theme	strain	1585:1590	arg1	strain					1631:1636	a reference strain	1619:1636	a reference strain	1619:1636	The type strain is 0911MAR22V3T (=LMG 27963T=CECT 8479T); and strain 0911TES10J4 (CECT 8478) is a reference strain.
29395538	4	44	theme	DSM	601:603	arg1	16318T					605:610	B. humi DSM 16318T	593:610	B. humi DSM 16318T	593:610	DNA-DNA hybridization values of the strain 0911MAR22V3T with respect to strain 0911TES10J4 and B. humi DSM 16318T were 76.8% (73.9%, reciprocal) and 56.9% (63.3%, reciprocal analysis), respectively.
29395538	5	45	theme	Whole	697:701	arg1	ANI					739:741	ANI	739:741	ANI	739:741	Whole genome average nucleotide identity (ANI) values of both strains were in the threshold value for species delineation and less than 85% with B. humi.
29395538	5	45	theme	Whole	697:701	arg1	identity					729:736	Whole genome average nucleotide identity	697:736	Whole genome average nucleotide identity (ANI) values of both strains	697:765	Whole genome average nucleotide identity (ANI) values of both strains were in the threshold value for species delineation and less than 85% with B. humi.
29395538	2	46	dep	Gram-positive	80:92	arg1	catalase-positive					95:111	catalase-positive	95:111	catalase-positive	95:111	Two Gram-positive, catalase-positive, oxidase-negative, motile, endospore-forming, rod-shaped bacteria, designated as 0911MAR22V3T and 0911TES10J4, were isolated from air samples collected in two show caves, located in Andalusia, Southern Spain.
29395538	2	46	dep	Gram-positive	80:92	arg1	rod-shaped					159:168	rod-shaped	159:168	rod-shaped	159:168	Two Gram-positive, catalase-positive, oxidase-negative, motile, endospore-forming, rod-shaped bacteria, designated as 0911MAR22V3T and 0911TES10J4, were isolated from air samples collected in two show caves, located in Andalusia, Southern Spain.
29395538	2	46	dep	Gram-positive	80:92	arg1	endospore-forming					140:156	endospore-forming	140:156	endospore-forming	140:156	Two Gram-positive, catalase-positive, oxidase-negative, motile, endospore-forming, rod-shaped bacteria, designated as 0911MAR22V3T and 0911TES10J4, were isolated from air samples collected in two show caves, located in Andalusia, Southern Spain.
29395538	2	46	dep	Gram-positive	80:92	arg1	motile					132:137	motile	132:137	motile	132:137	Two Gram-positive, catalase-positive, oxidase-negative, motile, endospore-forming, rod-shaped bacteria, designated as 0911MAR22V3T and 0911TES10J4, were isolated from air samples collected in two show caves, located in Andalusia, Southern Spain.
29395538	2	46	dep	Gram-positive	80:92	arg1	oxidase-negative					114:129	oxidase-negative	114:129	oxidase-negative	114:129	Two Gram-positive, catalase-positive, oxidase-negative, motile, endospore-forming, rod-shaped bacteria, designated as 0911MAR22V3T and 0911TES10J4, were isolated from air samples collected in two show caves, located in Andalusia, Southern Spain.
29395538	8	47	theme	phylogenetic	1285:1296	arg1	relatedness					1298:1308	their phylogenetic relatedness	1279:1308	their phylogenetic relatedness	1279:1308	On the basis of their phylogenetic relatedness and their phenotypic and genotypic features, the strains 0911MAR22V3T and 0911TES10J4 should be attributed to a novel species within the genus Bacillus, for which the name Bacillus onubensis sp.
29395538	3	48	theme	Bacillus	466:473	arg1	%					494:494	98%	492:494	98%	492:494	Phylogenetic analysis based on 16S rRNA gene sequences indicated that both strains were indistinguishable and they were most closely related to Bacillus humi DSM 16318T (98%).
29395538	3	48	theme	Bacillus	466:473	arg1	DSM					480:482	Bacillus humi DSM 16318T	466:489	Bacillus humi DSM 16318T (98%)	466:495	Phylogenetic analysis based on 16S rRNA gene sequences indicated that both strains were indistinguishable and they were most closely related to Bacillus humi DSM 16318T (98%).
29395538	5	49	theme	genome	703:708	arg1	ANI					739:741	ANI	739:741	ANI	739:741	Whole genome average nucleotide identity (ANI) values of both strains were in the threshold value for species delineation and less than 85% with B. humi.
29395538	5	49	theme	genome	703:708	arg1	identity					729:736	Whole genome average nucleotide identity	697:736	Whole genome average nucleotide identity (ANI) values of both strains	697:765	Whole genome average nucleotide identity (ANI) values of both strains were in the threshold value for species delineation and less than 85% with B. humi.
29395538	4	50	theme	DNA-DNA	498:504	arg1	hybridization					506:518	DNA-DNA hybridization	498:518	DNA-DNA hybridization values of the strain 0911MAR22V3T with respect to strain 0911TES10J4 and B. humi DSM 16318T	498:610	DNA-DNA hybridization values of the strain 0911MAR22V3T with respect to strain 0911TES10J4 and B. humi DSM 16318T were 76.8% (73.9%, reciprocal) and 56.9% (63.3%, reciprocal analysis), respectively.
29395538	7	51	theme	isoprenoid	1000:1009	arg1	quinone					1011:1017	the dominant isoprenoid quinone	987:1017	the dominant isoprenoid quinone	987:1017	In both strains the dominant isoprenoid quinone was MK-7, the major cellular polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and two more phospholipids, the predominant fatty acids were iso-C15:0 and anteiso-C15:0 and the DNA G+C content was 38mol%.
29395538	7	51	theme	isoprenoid	1000:1009	arg1	MK-7					1023:1026	MK-7	1023:1026	MK-7	1023:1026	In both strains the dominant isoprenoid quinone was MK-7, the major cellular polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and two more phospholipids, the predominant fatty acids were iso-C15:0 and anteiso-C15:0 and the DNA G+C content was 38mol%.
29395538	7	52	from	MK-7	1023:1026	arg1	strains					979:985	both strains	974:985	both strains	974:985	In both strains the dominant isoprenoid quinone was MK-7, the major cellular polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and two more phospholipids, the predominant fatty acids were iso-C15:0 and anteiso-C15:0 and the DNA G+C content was 38mol%.
29395538	10	53	theme	=LMG	1556:1559	arg1	8479T					1573:1577	=LMG 27963T=CECT 8479T	1556:1577	=LMG 27963T=CECT 8479T	1556:1577	The type strain is 0911MAR22V3T (=LMG 27963T=CECT 8479T); and strain 0911TES10J4 (CECT 8478) is a reference strain.
29395538	10	53	theme	=LMG	1556:1559	arg1	0911MAR22V3T					1542:1553	0911MAR22V3T	1542:1553	0911MAR22V3T (=LMG 27963T=CECT 8479T)	1542:1578	The type strain is 0911MAR22V3T (=LMG 27963T=CECT 8479T); and strain 0911TES10J4 (CECT 8478) is a reference strain.
29395538	2	54	theme	Southern	306:313	arg1	Andalusia					295:303	Andalusia	295:303	Andalusia	295:303	Two Gram-positive, catalase-positive, oxidase-negative, motile, endospore-forming, rod-shaped bacteria, designated as 0911MAR22V3T and 0911TES10J4, were isolated from air samples collected in two show caves, located in Andalusia, Southern Spain.
29395538	2	54	theme	Southern	306:313	arg1	Spain					315:319	Southern Spain	306:319	Southern Spain	306:319	Two Gram-positive, catalase-positive, oxidase-negative, motile, endospore-forming, rod-shaped bacteria, designated as 0911MAR22V3T and 0911TES10J4, were isolated from air samples collected in two show caves, located in Andalusia, Southern Spain.
29395538	10	55	theme	reference	1621:1629	arg1	strain					1631:1636	a reference strain	1619:1636	a reference strain	1619:1636	The type strain is 0911MAR22V3T (=LMG 27963T=CECT 8479T); and strain 0911TES10J4 (CECT 8478) is a reference strain.
29395538	10	55	theme	reference	1621:1629	arg1	0911TES10J4					1592:1602	strain 0911TES10J4	1585:1602	strain 0911TES10J4 (CECT 8478)	1585:1614	The type strain is 0911MAR22V3T (=LMG 27963T=CECT 8479T); and strain 0911TES10J4 (CECT 8478) is a reference strain.
29395538	4	56	theme	strain	570:575	arg1	0911TES10J4					577:587	strain 0911TES10J4	570:587	strain 0911TES10J4	570:587	DNA-DNA hybridization values of the strain 0911MAR22V3T with respect to strain 0911TES10J4 and B. humi DSM 16318T were 76.8% (73.9%, reciprocal) and 56.9% (63.3%, reciprocal analysis), respectively.
29395538	7	57	theme	38mol	1255:1259	arg1	content					1243:1249	the DNA G+C content	1231:1249	the DNA G+C content	1231:1249	In both strains the dominant isoprenoid quinone was MK-7, the major cellular polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and two more phospholipids, the predominant fatty acids were iso-C15:0 and anteiso-C15:0 and the DNA G+C content was 38mol%.
29395538	7	57	theme	38mol	1255:1259	arg1	%					1260:1260	38mol%	1255:1260	38mol%	1255:1260	In both strains the dominant isoprenoid quinone was MK-7, the major cellular polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and two more phospholipids, the predominant fatty acids were iso-C15:0 and anteiso-C15:0 and the DNA G+C content was 38mol%.
29395538	6	58	dep	Strains	851:857	arg1	0911TES10J4					876:886	0911TES10J4	876:886	0911TES10J4	876:886	Strains 0911MAR22V3T and 0911TES10J4 grew at 10-47°C (optimum 37°C), at pH 6-9.5 and with 0-8% (w/v) NaCl (optimum 1%).
29395538	6	58	dep	Strains	851:857	arg1	0911MAR22V3T					859:870	0911MAR22V3T	859:870	0911MAR22V3T	859:870	Strains 0911MAR22V3T and 0911TES10J4 grew at 10-47°C (optimum 37°C), at pH 6-9.5 and with 0-8% (w/v) NaCl (optimum 1%).
29395538	6	58	dep	Strains	851:857	arg1	Strains					851:857	Strains	851:857	Strains 0911MAR22V3T and 0911TES10J4	851:886	Strains 0911MAR22V3T and 0911TES10J4 grew at 10-47°C (optimum 37°C), at pH 6-9.5 and with 0-8% (w/v) NaCl (optimum 1%).
29395538	2	59	theme	air	243:245	arg1	samples					247:253	air samples	243:253	air samples collected in two show caves, located in Andalusia, Southern Spain	243:319	Two Gram-positive, catalase-positive, oxidase-negative, motile, endospore-forming, rod-shaped bacteria, designated as 0911MAR22V3T and 0911TES10J4, were isolated from air samples collected in two show caves, located in Andalusia, Southern Spain.
29395538	8	60	dep	strains	1359:1365	arg1	0911TES10J4					1384:1394	0911TES10J4	1384:1394	0911TES10J4	1384:1394	On the basis of their phylogenetic relatedness and their phenotypic and genotypic features, the strains 0911MAR22V3T and 0911TES10J4 should be attributed to a novel species within the genus Bacillus, for which the name Bacillus onubensis sp.
29395538	8	60	dep	strains	1359:1365	arg1	0911MAR22V3T					1367:1378	0911MAR22V3T	1367:1378	0911MAR22V3T	1367:1378	On the basis of their phylogenetic relatedness and their phenotypic and genotypic features, the strains 0911MAR22V3T and 0911TES10J4 should be attributed to a novel species within the genus Bacillus, for which the name Bacillus onubensis sp.
29395538	8	60	dep	strains	1359:1365	arg1	strains					1359:1365	the strains 0911MAR22V3T and 0911TES10J4	1355:1394	the strains 0911MAR22V3T and 0911TES10J4	1355:1394	On the basis of their phylogenetic relatedness and their phenotypic and genotypic features, the strains 0911MAR22V3T and 0911TES10J4 should be attributed to a novel species within the genus Bacillus, for which the name Bacillus onubensis sp.
29395538	5	61	theme	threshold	779:787	arg1	value					789:793	the threshold value	775:793	the threshold value for species delineation and less than 85% with B. humi	775:848	Whole genome average nucleotide identity (ANI) values of both strains were in the threshold value for species delineation and less than 85% with B. humi.
29395538	6	62	theme	optimum	958:964	arg1	NaCl					952:955	0-8% (w/v) NaCl	941:955	0-8% (w/v) NaCl (optimum 1%)	941:968	Strains 0911MAR22V3T and 0911TES10J4 grew at 10-47°C (optimum 37°C), at pH 6-9.5 and with 0-8% (w/v) NaCl (optimum 1%).
29395538	6	62	theme	optimum	958:964	arg1	%					967:967	optimum 1%	958:967	optimum 1%	958:967	Strains 0911MAR22V3T and 0911TES10J4 grew at 10-47°C (optimum 37°C), at pH 6-9.5 and with 0-8% (w/v) NaCl (optimum 1%).
29395538	8	63	theme	novel	1422:1426	arg1	species					1428:1434	a novel species	1420:1434	a novel species	1420:1434	On the basis of their phylogenetic relatedness and their phenotypic and genotypic features, the strains 0911MAR22V3T and 0911TES10J4 should be attributed to a novel species within the genus Bacillus, for which the name Bacillus onubensis sp.
29395538	7	64	theme	predominant	1170:1180	arg1	acids					1188:1192	the predominant fatty acids	1166:1192	the predominant fatty acids	1166:1192	In both strains the dominant isoprenoid quinone was MK-7, the major cellular polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and two more phospholipids, the predominant fatty acids were iso-C15:0 and anteiso-C15:0 and the DNA G+C content was 38mol%.
29395538	7	64	theme	predominant	1170:1180	arg1	iso-C15:0					1199:1207	iso-C15:0	1199:1207	iso-C15:0	1199:1207	In both strains the dominant isoprenoid quinone was MK-7, the major cellular polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and two more phospholipids, the predominant fatty acids were iso-C15:0 and anteiso-C15:0 and the DNA G+C content was 38mol%.
29395538	6	65	theme	w/v	947:949	arg1	NaCl					952:955	0-8% (w/v) NaCl	941:955	0-8% (w/v) NaCl (optimum 1%)	941:968	Strains 0911MAR22V3T and 0911TES10J4 grew at 10-47°C (optimum 37°C), at pH 6-9.5 and with 0-8% (w/v) NaCl (optimum 1%).
29395538	6	65	theme	w/v	947:949	arg1	%					967:967	optimum 1%	958:967	optimum 1%	958:967	Strains 0911MAR22V3T and 0911TES10J4 grew at 10-47°C (optimum 37°C), at pH 6-9.5 and with 0-8% (w/v) NaCl (optimum 1%).
29395538	8	66	theme	name	1477:1480	arg1	sp					1501:1502	name Bacillus onubensis sp	1477:1502	the name Bacillus onubensis sp	1473:1502	On the basis of their phylogenetic relatedness and their phenotypic and genotypic features, the strains 0911MAR22V3T and 0911TES10J4 should be attributed to a novel species within the genus Bacillus, for which the name Bacillus onubensis sp.
29395538	2	67	attach	isolated	229:236	arg2	bacteria					170:177	Two Gram-positive, catalase-positive, oxidase-negative, motile, endospore-forming, rod-shaped bacteria	76:177	Two Gram-positive, catalase-positive, oxidase-negative, motile, endospore-forming, rod-shaped bacteria	76:177	Two Gram-positive, catalase-positive, oxidase-negative, motile, endospore-forming, rod-shaped bacteria, designated as 0911MAR22V3T and 0911TES10J4, were isolated from air samples collected in two show caves, located in Andalusia, Southern Spain.
29395538	2	67	attach	isolated	229:236	arg1	samples					247:253	air samples	243:253	air samples collected in two show caves, located in Andalusia, Southern Spain	243:319	Two Gram-positive, catalase-positive, oxidase-negative, motile, endospore-forming, rod-shaped bacteria, designated as 0911MAR22V3T and 0911TES10J4, were isolated from air samples collected in two show caves, located in Andalusia, Southern Spain.
29395538	7	68	theme	major	1033:1037	arg1	lipids					1054:1059	the major cellular polar lipids	1029:1059	the major cellular polar lipids	1029:1059	In both strains the dominant isoprenoid quinone was MK-7, the major cellular polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and two more phospholipids, the predominant fatty acids were iso-C15:0 and anteiso-C15:0 and the DNA G+C content was 38mol%.
29395538	7	68	theme	major	1033:1037	arg1	diphosphatidylglycerol					1066:1087	diphosphatidylglycerol	1066:1087	diphosphatidylglycerol	1066:1087	In both strains the dominant isoprenoid quinone was MK-7, the major cellular polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and two more phospholipids, the predominant fatty acids were iso-C15:0 and anteiso-C15:0 and the DNA G+C content was 38mol%.
29395538	8	69	theme	phenotypic	1320:1329	arg1	features					1345:1352	their phenotypic and genotypic features	1314:1352	their phenotypic and genotypic features	1314:1352	On the basis of their phylogenetic relatedness and their phenotypic and genotypic features, the strains 0911MAR22V3T and 0911TES10J4 should be attributed to a novel species within the genus Bacillus, for which the name Bacillus onubensis sp.
29395538	7	70	theme	fatty	1182:1186	arg1	acids					1188:1192	the predominant fatty acids	1166:1192	the predominant fatty acids	1166:1192	In both strains the dominant isoprenoid quinone was MK-7, the major cellular polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and two more phospholipids, the predominant fatty acids were iso-C15:0 and anteiso-C15:0 and the DNA G+C content was 38mol%.
29395538	7	70	theme	fatty	1182:1186	arg1	iso-C15:0					1199:1207	iso-C15:0	1199:1207	iso-C15:0	1199:1207	In both strains the dominant isoprenoid quinone was MK-7, the major cellular polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and two more phospholipids, the predominant fatty acids were iso-C15:0 and anteiso-C15:0 and the DNA G+C content was 38mol%.
29395538	4	71	dep	%	621:621	arg1	%					628:628	73.9%	624:628	73.9%	624:628	DNA-DNA hybridization values of the strain 0911MAR22V3T with respect to strain 0911TES10J4 and B. humi DSM 16318T were 76.8% (73.9%, reciprocal) and 56.9% (63.3%, reciprocal analysis), respectively.
29395538	3	72	theme	rRNA	357:360	arg1	sequences					367:375	16S rRNA gene sequences	353:375	16S rRNA gene sequences	353:375	Phylogenetic analysis based on 16S rRNA gene sequences indicated that both strains were indistinguishable and they were most closely related to Bacillus humi DSM 16318T (98%).
29395538	7	73	theme	cellular	1039:1046	arg1	lipids					1054:1059	the major cellular polar lipids	1029:1059	the major cellular polar lipids	1029:1059	In both strains the dominant isoprenoid quinone was MK-7, the major cellular polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and two more phospholipids, the predominant fatty acids were iso-C15:0 and anteiso-C15:0 and the DNA G+C content was 38mol%.
29395538	7	73	theme	cellular	1039:1046	arg1	diphosphatidylglycerol					1066:1087	diphosphatidylglycerol	1066:1087	diphosphatidylglycerol	1066:1087	In both strains the dominant isoprenoid quinone was MK-7, the major cellular polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and two more phospholipids, the predominant fatty acids were iso-C15:0 and anteiso-C15:0 and the DNA G+C content was 38mol%.
29606765	7	0	theme	wall	1097:1100	arg1	polysaccharides					1102:1116	the structural cell wall polysaccharides	1077:1116	the structural cell wall polysaccharides	1077:1116	The multi-enzyme complex Viscozyme hydrolyzed the structural cell wall polysaccharides, improving extraction and obtaining protein concentrate from the okara.
29606765	5	1	theme	protein	748:754	arg1	concentrate					756:766	protein concentrate	748:766	protein concentrate	748:766	The optimal conditions for maximum protein content and recovery in protein concentrate were 53 °C, pH 6.2 and 4% of enzyme concentration.
29606765	6	2	theme	%	864:864	arg1	content					851:857	protein content	843:857	protein content of 56% (dry weight basis)	843:883	Under these conditions, protein content of 56% (dry weight basis) and a recovery of 28% were obtained, representing an increase of 17 and 86%, respectively, compared to the sample with no enzymatic pretreatment.
29606765	6	2	theme	%	864:864	arg1	recovery					891:898	a recovery	889:898	a recovery of 28%	889:905	Under these conditions, protein content of 56% (dry weight basis) and a recovery of 28% were obtained, representing an increase of 17 and 86%, respectively, compared to the sample with no enzymatic pretreatment.
29606765	4	3	theme	isoelectric	654:664	arg1	precipitation					666:678	isoelectric precipitation	654:678	isoelectric precipitation	654:678	After extraction, the protein was concentrated by isoelectric precipitation.
29606765	5	4	from	conditions	693:702	arg1	concentrate					756:766	protein concentrate	748:766	protein concentrate	748:766	The optimal conditions for maximum protein content and recovery in protein concentrate were 53 °C, pH 6.2 and 4% of enzyme concentration.
29606765	0	5	from	Action	0:5	arg1	extraction					42:51	protein extraction	34:51	protein extraction	34:51	Action of multi-enzyme complex on protein extraction to obtain a protein concentrate from okara.
29606765	2	6	used	used	336:339	arg2	Viscozyme					282:290	The multi-enzyme complex Viscozyme	257:290	The multi-enzyme complex Viscozyme	257:290	The multi-enzyme complex Viscozyme, containing a variety of carbohydrases, was used to hydrolyze the okara cell walls and facilitate extraction of proteins.
29606765	2	7	theme	complex	274:280	arg1	Viscozyme					282:290	The multi-enzyme complex Viscozyme	257:290	The multi-enzyme complex Viscozyme	257:290	The multi-enzyme complex Viscozyme, containing a variety of carbohydrases, was used to hydrolyze the okara cell walls and facilitate extraction of proteins.
29606765	8	8	theme	basic	1303:1307	arg1	subunits					1309:1316	the acidic and basic subunits	1288:1316	the acidic and basic subunits of the protein glycinin	1288:1340	An electrophoretic profile of the protein concentrate showed two distinct bands, corresponding to the acidic and basic subunits of the protein glycinin.
29606765	1	9	theme	soymilk	237:243	arg1	processing					245:254	soymilk processing	237:254	soymilk processing	237:254	The objective of this study was to optimize the extraction of protein by applying a multi-enzymatic pretreatment to okara, a byproduct from soymilk processing.
29606765	5	10	theme	maximum	708:714	arg1	content					724:730	maximum protein content	708:730	maximum protein content	708:730	The optimal conditions for maximum protein content and recovery in protein concentrate were 53 °C, pH 6.2 and 4% of enzyme concentration.
29606765	9	11	theme	greater	1418:1424	arg1	content					1426:1432	a greater content	1416:1432	a greater content of arginine	1416:1444	There were no limiting amino acids in the protein concentrate, which had a greater content of arginine.
29606765	2	12	theme	multi-enzyme	261:272	arg1	Viscozyme					282:290	The multi-enzyme complex Viscozyme	257:290	The multi-enzyme complex Viscozyme	257:290	The multi-enzyme complex Viscozyme, containing a variety of carbohydrases, was used to hydrolyze the okara cell walls and facilitate extraction of proteins.
29606765	6	13	theme	%	905:905	arg1	content					851:857	protein content	843:857	protein content of 56% (dry weight basis)	843:883	Under these conditions, protein content of 56% (dry weight basis) and a recovery of 28% were obtained, representing an increase of 17 and 86%, respectively, compared to the sample with no enzymatic pretreatment.
29606765	6	13	theme	%	905:905	arg1	recovery					891:898	a recovery	889:898	a recovery of 28%	889:905	Under these conditions, protein content of 56% (dry weight basis) and a recovery of 28% were obtained, representing an increase of 17 and 86%, respectively, compared to the sample with no enzymatic pretreatment.
29606765	5	14	theme	protein	716:722	arg1	content					724:730	maximum protein content	708:730	maximum protein content	708:730	The optimal conditions for maximum protein content and recovery in protein concentrate were 53 °C, pH 6.2 and 4% of enzyme concentration.
29606765	0	15	from	extraction	42:51	arg1	Action					0:5	Action	0:5	Action of multi-enzyme complex on protein extraction to obtain a protein	0:71	Action of multi-enzyme complex on protein extraction to obtain a protein concentrate from okara.
29606765	9	16	contain	had	1412:1414	arg1	acids					1372:1376	no limiting amino acids	1354:1376	no limiting amino acids	1354:1376	There were no limiting amino acids in the protein concentrate, which had a greater content of arginine.
29606765	9	16	contain	had	1412:1414	arg2	content					1426:1432	a greater content	1416:1432	a greater content of arginine	1416:1444	There were no limiting amino acids in the protein concentrate, which had a greater content of arginine.
29606765	1	17	theme	protein	159:165	arg1	extraction					145:154	the extraction	141:154	the extraction of protein	141:165	The objective of this study was to optimize the extraction of protein by applying a multi-enzymatic pretreatment to okara, a byproduct from soymilk processing.
29606765	3	18	theme	Enzyme-assisted	414:428	arg1	extraction					430:439	Enzyme-assisted extraction	414:439	Enzyme-assisted extraction	414:439	Enzyme-assisted extraction was carried out under different temperatures (37-53 °C), enzyme concentrations (1.5-4%) and pH values (5.5-6.5) according to a central composite rotatable design.
29606765	6	19	theme	protein	843:849	arg1	content					851:857	protein content	843:857	protein content of 56% (dry weight basis)	843:883	Under these conditions, protein content of 56% (dry weight basis) and a recovery of 28% were obtained, representing an increase of 17 and 86%, respectively, compared to the sample with no enzymatic pretreatment.
29606765	7	20	theme	complex	1048:1054	arg1	Viscozyme					1056:1064	The multi-enzyme complex Viscozyme	1031:1064	The multi-enzyme complex Viscozyme	1031:1064	The multi-enzyme complex Viscozyme hydrolyzed the structural cell wall polysaccharides, improving extraction and obtaining protein concentrate from the okara.
29606765	2	21	theme	cell	364:367	arg1	walls					369:373	the okara cell walls	354:373	the okara cell walls	354:373	The multi-enzyme complex Viscozyme, containing a variety of carbohydrases, was used to hydrolyze the okara cell walls and facilitate extraction of proteins.
29606765	7	22	theme	structural	1081:1090	arg1	polysaccharides					1102:1116	the structural cell wall polysaccharides	1077:1116	the structural cell wall polysaccharides	1077:1116	The multi-enzyme complex Viscozyme hydrolyzed the structural cell wall polysaccharides, improving extraction and obtaining protein concentrate from the okara.
29606765	9	23	theme	arginine	1437:1444	arg1	content					1426:1432	a greater content	1416:1432	a greater content of arginine	1416:1444	There were no limiting amino acids in the protein concentrate, which had a greater content of arginine.
29606765	7	24	dep	polysaccharides	1102:1116	arg1	improving					1119:1127	improving	1119:1127	improving extraction	1119:1138	The multi-enzyme complex Viscozyme hydrolyzed the structural cell wall polysaccharides, improving extraction and obtaining protein concentrate from the okara.
29606765	7	24	dep	polysaccharides	1102:1116	arg1	obtaining					1144:1152	obtaining	1144:1152	obtaining protein concentrate from the okara	1144:1187	The multi-enzyme complex Viscozyme hydrolyzed the structural cell wall polysaccharides, improving extraction and obtaining protein concentrate from the okara.
29606765	2	25	theme	okara	358:362	arg1	walls					369:373	the okara cell walls	354:373	the okara cell walls	354:373	The multi-enzyme complex Viscozyme, containing a variety of carbohydrases, was used to hydrolyze the okara cell walls and facilitate extraction of proteins.
29606765	7	26	theme	cell	1092:1095	arg1	polysaccharides					1102:1116	the structural cell wall polysaccharides	1077:1116	the structural cell wall polysaccharides	1077:1116	The multi-enzyme complex Viscozyme hydrolyzed the structural cell wall polysaccharides, improving extraction and obtaining protein concentrate from the okara.
29606765	6	27	theme	weight	871:876	arg1	%					864:864	56%	862:864	56% (dry weight basis)	862:883	Under these conditions, protein content of 56% (dry weight basis) and a recovery of 28% were obtained, representing an increase of 17 and 86%, respectively, compared to the sample with no enzymatic pretreatment.
29606765	6	27	theme	weight	871:876	arg1	basis					878:882	dry weight basis	867:882	dry weight basis	867:882	Under these conditions, protein content of 56% (dry weight basis) and a recovery of 28% were obtained, representing an increase of 17 and 86%, respectively, compared to the sample with no enzymatic pretreatment.
29606765	8	28	theme	acidic	1292:1297	arg1	subunits					1309:1316	the acidic and basic subunits	1288:1316	the acidic and basic subunits of the protein glycinin	1288:1340	An electrophoretic profile of the protein concentrate showed two distinct bands, corresponding to the acidic and basic subunits of the protein glycinin.
29606765	0	29	theme	complex	23:29	arg1	Action					0:5	Action	0:5	Action of multi-enzyme complex on protein extraction to obtain a protein	0:71	Action of multi-enzyme complex on protein extraction to obtain a protein concentrate from okara.
29606765	8	30	theme	concentrate	1232:1242	arg1	profile					1209:1215	An electrophoretic profile	1190:1215	An electrophoretic profile of the protein concentrate	1190:1242	An electrophoretic profile of the protein concentrate showed two distinct bands, corresponding to the acidic and basic subunits of the protein glycinin.
29606765	8	31	theme	protein	1224:1230	arg1	concentrate					1232:1242	the protein concentrate	1220:1242	the protein concentrate	1220:1242	An electrophoretic profile of the protein concentrate showed two distinct bands, corresponding to the acidic and basic subunits of the protein glycinin.
29606765	8	32	theme	distinct	1255:1262	arg1	bands					1264:1268	two distinct bands	1251:1268	two distinct bands	1251:1268	An electrophoretic profile of the protein concentrate showed two distinct bands, corresponding to the acidic and basic subunits of the protein glycinin.
29606765	6	33	theme	%	959:959	arg1	increase					938:945	an increase	935:945	an increase	935:945	Under these conditions, protein content of 56% (dry weight basis) and a recovery of 28% were obtained, representing an increase of 17 and 86%, respectively, compared to the sample with no enzymatic pretreatment.
29606765	2	34	theme	proteins	404:411	arg1	extraction					390:399	extraction	390:399	extraction of proteins	390:411	The multi-enzyme complex Viscozyme, containing a variety of carbohydrases, was used to hydrolyze the okara cell walls and facilitate extraction of proteins.
29606765	1	35	from	processing	245:254	arg1	okara					213:217	okara	213:217	okara	213:217	The objective of this study was to optimize the extraction of protein by applying a multi-enzymatic pretreatment to okara, a byproduct from soymilk processing.
29606765	1	35	from	processing	245:254	arg1	byproduct					222:230	a byproduct	220:230	a byproduct from soymilk processing	220:254	The objective of this study was to optimize the extraction of protein by applying a multi-enzymatic pretreatment to okara, a byproduct from soymilk processing.
29606765	3	36	theme	pH	533:534	arg1	5.5-6.5					544:550	5.5-6.5	544:550	5.5-6.5	544:550	Enzyme-assisted extraction was carried out under different temperatures (37-53 °C), enzyme concentrations (1.5-4%) and pH values (5.5-6.5) according to a central composite rotatable design.
29606765	3	36	theme	pH	533:534	arg1	values					536:541	pH values	533:541	pH values (5.5-6.5)	533:551	Enzyme-assisted extraction was carried out under different temperatures (37-53 °C), enzyme concentrations (1.5-4%) and pH values (5.5-6.5) according to a central composite rotatable design.
29606765	9	37	theme	protein	1385:1391	arg1	concentrate					1393:1403	the protein concentrate	1381:1403	the protein concentrate	1381:1403	There were no limiting amino acids in the protein concentrate, which had a greater content of arginine.
29606765	0	38	theme	protein	34:40	arg1	extraction					42:51	protein extraction	34:51	protein extraction	34:51	Action of multi-enzyme complex on protein extraction to obtain a protein concentrate from okara.
29606765	5	39	theme	optimal	685:691	arg1	53 °C					773:777	53 °C	773:777	53 °C	773:777	The optimal conditions for maximum protein content and recovery in protein concentrate were 53 °C, pH 6.2 and 4% of enzyme concentration.
29606765	5	39	theme	optimal	685:691	arg1	conditions					693:702	The optimal conditions	681:702	The optimal conditions for maximum protein content and recovery in protein concentrate	681:766	The optimal conditions for maximum protein content and recovery in protein concentrate were 53 °C, pH 6.2 and 4% of enzyme concentration.
29606765	5	40	theme	enzyme	797:802	arg1	concentration					804:816	enzyme concentration	797:816	enzyme concentration	797:816	The optimal conditions for maximum protein content and recovery in protein concentrate were 53 °C, pH 6.2 and 4% of enzyme concentration.
29606765	1	41	theme	multi-enzymatic	181:195	arg1	pretreatment					197:208	a multi-enzymatic pretreatment	179:208	a multi-enzymatic pretreatment	179:208	The objective of this study was to optimize the extraction of protein by applying a multi-enzymatic pretreatment to okara, a byproduct from soymilk processing.
29606765	9	42	theme	limiting	1357:1364	arg1	acids					1372:1376	no limiting amino acids	1354:1376	no limiting amino acids	1354:1376	There were no limiting amino acids in the protein concentrate, which had a greater content of arginine.
29606765	5	43	theme	concentration	804:816	arg1	53 °C					773:777	53 °C	773:777	53 °C	773:777	The optimal conditions for maximum protein content and recovery in protein concentrate were 53 °C, pH 6.2 and 4% of enzyme concentration.
29606765	5	43	theme	concentration	804:816	arg1	%					792:792	4%	791:792	4% of enzyme concentration	791:816	The optimal conditions for maximum protein content and recovery in protein concentrate were 53 °C, pH 6.2 and 4% of enzyme concentration.
29606765	5	43	theme	concentration	804:816	arg1	concentration					804:816	enzyme concentration	797:816	enzyme concentration	797:816	The optimal conditions for maximum protein content and recovery in protein concentrate were 53 °C, pH 6.2 and 4% of enzyme concentration.
29606765	5	43	theme	concentration	804:816	arg1	pH					780:781	pH 6.2	780:785	pH 6.2	780:785	The optimal conditions for maximum protein content and recovery in protein concentrate were 53 °C, pH 6.2 and 4% of enzyme concentration.
29606765	5	43	theme	concentration	804:816	arg1	conditions					693:702	The optimal conditions	681:702	The optimal conditions for maximum protein content and recovery in protein concentrate	681:766	The optimal conditions for maximum protein content and recovery in protein concentrate were 53 °C, pH 6.2 and 4% of enzyme concentration.
29606765	8	44	theme	glycinin	1333:1340	arg1	subunits					1309:1316	the acidic and basic subunits	1288:1316	the acidic and basic subunits of the protein glycinin	1288:1340	An electrophoretic profile of the protein concentrate showed two distinct bands, corresponding to the acidic and basic subunits of the protein glycinin.
29606765	7	45	theme	multi-enzyme	1035:1046	arg1	Viscozyme					1056:1064	The multi-enzyme complex Viscozyme	1031:1064	The multi-enzyme complex Viscozyme	1031:1064	The multi-enzyme complex Viscozyme hydrolyzed the structural cell wall polysaccharides, improving extraction and obtaining protein concentrate from the okara.
29606765	2	46	contain	containing	293:302	arg2	variety					306:312	a variety	304:312	a variety of carbohydrases	304:329	The multi-enzyme complex Viscozyme, containing a variety of carbohydrases, was used to hydrolyze the okara cell walls and facilitate extraction of proteins.
29606765	2	46	contain	containing	293:302	arg1	Viscozyme					282:290	The multi-enzyme complex Viscozyme	257:290	The multi-enzyme complex Viscozyme	257:290	The multi-enzyme complex Viscozyme, containing a variety of carbohydrases, was used to hydrolyze the okara cell walls and facilitate extraction of proteins.
29606765	2	46	contain	containing	293:302	arg2	carbohydrases					317:329	carbohydrases	317:329	carbohydrases	317:329	The multi-enzyme complex Viscozyme, containing a variety of carbohydrases, was used to hydrolyze the okara cell walls and facilitate extraction of proteins.
29606765	6	47	theme	dry	867:869	arg1	%					864:864	56%	862:864	56% (dry weight basis)	862:883	Under these conditions, protein content of 56% (dry weight basis) and a recovery of 28% were obtained, representing an increase of 17 and 86%, respectively, compared to the sample with no enzymatic pretreatment.
29606765	6	47	theme	dry	867:869	arg1	basis					878:882	dry weight basis	867:882	dry weight basis	867:882	Under these conditions, protein content of 56% (dry weight basis) and a recovery of 28% were obtained, representing an increase of 17 and 86%, respectively, compared to the sample with no enzymatic pretreatment.
29606765	8	48	theme	protein	1325:1331	arg1	glycinin					1333:1340	the protein glycinin	1321:1340	the protein glycinin	1321:1340	An electrophoretic profile of the protein concentrate showed two distinct bands, corresponding to the acidic and basic subunits of the protein glycinin.
29606765	2	49	theme	carbohydrases	317:329	arg1	carbohydrases					317:329	carbohydrases	317:329	carbohydrases	317:329	The multi-enzyme complex Viscozyme, containing a variety of carbohydrases, was used to hydrolyze the okara cell walls and facilitate extraction of proteins.
29606765	2	49	theme	carbohydrases	317:329	arg1	variety					306:312	a variety	304:312	a variety of carbohydrases	304:329	The multi-enzyme complex Viscozyme, containing a variety of carbohydrases, was used to hydrolyze the okara cell walls and facilitate extraction of proteins.
29606765	3	50	theme	central	568:574	arg1	design					596:601	a central composite rotatable design	566:601	a central composite rotatable design	566:601	Enzyme-assisted extraction was carried out under different temperatures (37-53 °C), enzyme concentrations (1.5-4%) and pH values (5.5-6.5) according to a central composite rotatable design.
29606765	3	51	theme	composite	576:584	arg1	design					596:601	a central composite rotatable design	566:601	a central composite rotatable design	566:601	Enzyme-assisted extraction was carried out under different temperatures (37-53 °C), enzyme concentrations (1.5-4%) and pH values (5.5-6.5) according to a central composite rotatable design.
29606765	3	52	theme	different	463:471	arg1	37-53 °C					487:494	37-53 °C	487:494	37-53 °C	487:494	Enzyme-assisted extraction was carried out under different temperatures (37-53 °C), enzyme concentrations (1.5-4%) and pH values (5.5-6.5) according to a central composite rotatable design.
29606765	3	52	theme	different	463:471	arg1	temperatures					473:484	different temperatures	463:484	different temperatures (37-53 °C)	463:495	Enzyme-assisted extraction was carried out under different temperatures (37-53 °C), enzyme concentrations (1.5-4%) and pH values (5.5-6.5) according to a central composite rotatable design.
29606765	8	53	theme	electrophoretic	1193:1207	arg1	profile					1209:1215	An electrophoretic profile	1190:1215	An electrophoretic profile of the protein concentrate	1190:1242	An electrophoretic profile of the protein concentrate showed two distinct bands, corresponding to the acidic and basic subunits of the protein glycinin.
29606765	3	54	theme	rotatable	586:594	arg1	design					596:601	a central composite rotatable design	566:601	a central composite rotatable design	566:601	Enzyme-assisted extraction was carried out under different temperatures (37-53 °C), enzyme concentrations (1.5-4%) and pH values (5.5-6.5) according to a central composite rotatable design.
29606765	9	55	from	acids	1372:1376	arg1	concentrate					1393:1403	the protein concentrate	1381:1403	the protein concentrate	1381:1403	There were no limiting amino acids in the protein concentrate, which had a greater content of arginine.
29606765	1	56	theme	study	119:123	arg1	objective					101:109	The objective	97:109	The objective of this study	97:123	The objective of this study was to optimize the extraction of protein by applying a multi-enzymatic pretreatment to okara, a byproduct from soymilk processing.
29606765	6	57	theme	enzymatic	1007:1015	arg1	pretreatment					1017:1028	no enzymatic pretreatment	1004:1028	no enzymatic pretreatment	1004:1028	Under these conditions, protein content of 56% (dry weight basis) and a recovery of 28% were obtained, representing an increase of 17 and 86%, respectively, compared to the sample with no enzymatic pretreatment.
29606765	6	58	theme	17	950:951	arg1	increase					938:945	an increase	935:945	an increase	935:945	Under these conditions, protein content of 56% (dry weight basis) and a recovery of 28% were obtained, representing an increase of 17 and 86%, respectively, compared to the sample with no enzymatic pretreatment.
29606765	3	59	theme	enzyme	498:503	arg1	%					526:526	1.5-4%	521:526	1.5-4%	521:526	Enzyme-assisted extraction was carried out under different temperatures (37-53 °C), enzyme concentrations (1.5-4%) and pH values (5.5-6.5) according to a central composite rotatable design.
29606765	3	59	theme	enzyme	498:503	arg1	concentrations					505:518	enzyme concentrations	498:518	enzyme concentrations (1.5-4%)	498:527	Enzyme-assisted extraction was carried out under different temperatures (37-53 °C), enzyme concentrations (1.5-4%) and pH values (5.5-6.5) according to a central composite rotatable design.
29606765	9	60	theme	amino	1366:1370	arg1	acids					1372:1376	no limiting amino acids	1354:1376	no limiting amino acids	1354:1376	There were no limiting amino acids in the protein concentrate, which had a greater content of arginine.
29606765	7	61	theme	protein	1154:1160	arg1	concentrate					1162:1172	protein concentrate	1154:1172	protein concentrate	1154:1172	The multi-enzyme complex Viscozyme hydrolyzed the structural cell wall polysaccharides, improving extraction and obtaining protein concentrate from the okara.
29606765	0	62	from	complex	23:29	arg1	extraction					42:51	protein extraction	34:51	protein extraction	34:51	Action of multi-enzyme complex on protein extraction to obtain a protein concentrate from okara.
29606765	0	63	theme	multi-enzyme	10:21	arg1	complex					23:29	multi-enzyme complex	10:29	multi-enzyme complex on protein extraction to obtain a protein	10:71	Action of multi-enzyme complex on protein extraction to obtain a protein concentrate from okara.
31152813	0	0	theme	antioxidant	73:83	arg1	activities					108:117	its antioxidant, and anti-inflammatory activities	69:117	its antioxidant, and anti-inflammatory activities	69:117	Phytochemical profile of hot water extract of Glechoma hederacea and its antioxidant, and anti-inflammatory activities.
31152813	5	1	theme	HWG	737:739	arg1	activities					723:732	The antioxidant and anti-inflammatory activities	685:732	The antioxidant and anti-inflammatory activities of HWG	685:739	The antioxidant and anti-inflammatory activities of HWG were determined in vitro in lipopolysaccharide (LPS)-activated RAW 264.7 macrophages.
31152813	1	2	theme	Labiatae	154:161	arg1	family					163:168	the Labiatae family	150:168	the Labiatae family	150:168	Glechoma hederacea belongs to the Labiatae family and has many biological activities.
31152813	4	3	theme	scavenging	540:549	arg1	capacity					551:558	The free radical scavenging capacity	523:558	The free radical scavenging capacity of HWG in cell-free systems	523:586	The free radical scavenging capacity of HWG in cell-free systems was evaluated by using the α,α-diphenyl-β-picrylhydrazyl (DPPH) and β-carotene bleaching assays.
31152813	4	4	from	HWG	563:565	arg1	systems					580:586	cell-free systems	570:586	cell-free systems	570:586	The free radical scavenging capacity of HWG in cell-free systems was evaluated by using the α,α-diphenyl-β-picrylhydrazyl (DPPH) and β-carotene bleaching assays.
31152813	6	5	theme	comet	876:880	arg1	assay					882:886	the comet assay	872:886	the comet assay	872:886	The results demonstrated that DAPI staining, the comet assay, and DNA fragmentation showed that HWG prevented LPS-induced DNA damage in RAW264.7 macrophages, reduced the content of LPS-induced nitric oxide (NO) and malondialdehyde (MDA), increased GSH levels, and regulated antioxidant enzyme activities.
31152813	8	6	theme	antioxidant	1439:1449	arg1	properties					1473:1482	potent antioxidant and anti-inflammatory properties	1432:1482	potent antioxidant and anti-inflammatory properties	1432:1482	These results show that HWG and its main components possess potent antioxidant and anti-inflammatory properties.
31152813	2	7	theme	G.	347:348	arg1	hederacea					350:358	G. hederacea	347:358	G. hederacea (HWG)	347:364	The objective of this study was to evaluate the chemical composition, antioxidant and anti-inflammatory activities of a hot water extract of G. hederacea (HWG).
31152813	2	7	theme	G.	347:348	arg1	HWG					361:363	HWG	361:363	HWG	361:363	The objective of this study was to evaluate the chemical composition, antioxidant and anti-inflammatory activities of a hot water extract of G. hederacea (HWG).
31152813	3	8	from	phytochemicals	500:513	arg1	HWG					518:520	HWG	518:520	HWG	518:520	Our results indicated that rosmarinic acid, chlorogenic acid, caffeic acid, rutin, genistin, and ferulic acid were the most abundant phytochemicals in HWG.
31152813	4	9	theme	radical	532:538	arg1	scavenging					540:549	free radical scavenging	527:549	The free radical scavenging capacity of HWG in cell-free systems	523:586	The free radical scavenging capacity of HWG in cell-free systems was evaluated by using the α,α-diphenyl-β-picrylhydrazyl (DPPH) and β-carotene bleaching assays.
31152813	6	10	theme	malondialdehyde	1042:1056	arg1	content					997:1003	the content	993:1003	the content of LPS-induced nitric oxide (NO) and malondialdehyde (MDA)	993:1062	The results demonstrated that DAPI staining, the comet assay, and DNA fragmentation showed that HWG prevented LPS-induced DNA damage in RAW264.7 macrophages, reduced the content of LPS-induced nitric oxide (NO) and malondialdehyde (MDA), increased GSH levels, and regulated antioxidant enzyme activities.
31152813	8	11	theme	potent	1432:1437	arg1	properties					1473:1482	potent antioxidant and anti-inflammatory properties	1432:1482	potent antioxidant and anti-inflammatory properties	1432:1482	These results show that HWG and its main components possess potent antioxidant and anti-inflammatory properties.
31152813	7	12	from	expression	1207:1216	arg1	macrophages					1269:1279	RAW264.7 macrophages	1260:1279	RAW264.7 macrophages	1260:1279	We also demonstrated that HWG significantly decreased the LPS-induced mRNA expression of iNOS, COX-2, TNF-α, IL-6, and IL-1β in RAW264.7 macrophages, and reduced the LPS-induced protein expression of iNOS and COX-2 in RAW264.7 macrophages.
31152813	7	13	theme	iNOS	1332:1335	arg1	expression					1318:1327	the LPS-induced protein expression	1294:1327	the LPS-induced protein expression of iNOS and COX-2 in RAW264.7 macrophages	1294:1369	We also demonstrated that HWG significantly decreased the LPS-induced mRNA expression of iNOS, COX-2, TNF-α, IL-6, and IL-1β in RAW264.7 macrophages, and reduced the LPS-induced protein expression of iNOS and COX-2 in RAW264.7 macrophages.
31152813	4	14	from	capacity	551:558	arg1	systems					580:586	cell-free systems	570:586	cell-free systems	570:586	The free radical scavenging capacity of HWG in cell-free systems was evaluated by using the α,α-diphenyl-β-picrylhydrazyl (DPPH) and β-carotene bleaching assays.
31152813	8	15	theme	anti-inflammatory	1455:1471	arg1	properties					1473:1482	potent antioxidant and anti-inflammatory properties	1432:1482	potent antioxidant and anti-inflammatory properties	1432:1482	These results show that HWG and its main components possess potent antioxidant and anti-inflammatory properties.
31152813	7	16	theme	mRNA	1202:1205	arg1	expression					1207:1216	the LPS-induced mRNA expression	1186:1216	the LPS-induced mRNA expression of iNOS, COX-2, TNF-α, IL-6, and IL-1β in RAW264.7 macrophages	1186:1279	We also demonstrated that HWG significantly decreased the LPS-induced mRNA expression of iNOS, COX-2, TNF-α, IL-6, and IL-1β in RAW264.7 macrophages, and reduced the LPS-induced protein expression of iNOS and COX-2 in RAW264.7 macrophages.
31152813	2	17	theme	hederacea	350:358	arg1	antioxidant					276:286	antioxidant	276:286	antioxidant	276:286	The objective of this study was to evaluate the chemical composition, antioxidant and anti-inflammatory activities of a hot water extract of G. hederacea (HWG).
31152813	2	17	theme	hederacea	350:358	arg1	activities					310:319	anti-inflammatory activities	292:319	anti-inflammatory activities	292:319	The objective of this study was to evaluate the chemical composition, antioxidant and anti-inflammatory activities of a hot water extract of G. hederacea (HWG).
31152813	2	17	theme	hederacea	350:358	arg1	composition					263:273	the chemical composition	250:273	the chemical composition	250:273	The objective of this study was to evaluate the chemical composition, antioxidant and anti-inflammatory activities of a hot water extract of G. hederacea (HWG).
31152813	6	18	theme	DNA	949:951	arg1	damage					953:958	LPS-induced DNA damage	937:958	LPS-induced DNA damage in RAW264.7 macrophages	937:982	The results demonstrated that DAPI staining, the comet assay, and DNA fragmentation showed that HWG prevented LPS-induced DNA damage in RAW264.7 macrophages, reduced the content of LPS-induced nitric oxide (NO) and malondialdehyde (MDA), increased GSH levels, and regulated antioxidant enzyme activities.
31152813	0	19	theme	activities	108:117	arg1	profile					14:20	Phytochemical profile	0:20	Phytochemical profile of hot water extract of Glechoma hederacea and its antioxidant, and anti-inflammatory activities.	0:118	Phytochemical profile of hot water extract of Glechoma hederacea and its antioxidant, and anti-inflammatory activities.
31152813	6	20	theme	GSH	1075:1077	arg1	levels					1079:1084	GSH levels	1075:1084	GSH levels	1075:1084	The results demonstrated that DAPI staining, the comet assay, and DNA fragmentation showed that HWG prevented LPS-induced DNA damage in RAW264.7 macrophages, reduced the content of LPS-induced nitric oxide (NO) and malondialdehyde (MDA), increased GSH levels, and regulated antioxidant enzyme activities.
31152813	6	21	from	damage	953:958	arg1	macrophages					972:982	RAW264.7 macrophages	963:982	RAW264.7 macrophages	963:982	The results demonstrated that DAPI staining, the comet assay, and DNA fragmentation showed that HWG prevented LPS-induced DNA damage in RAW264.7 macrophages, reduced the content of LPS-induced nitric oxide (NO) and malondialdehyde (MDA), increased GSH levels, and regulated antioxidant enzyme activities.
31152813	6	22	theme	LPS-induced	1008:1018	arg1	NO					1034:1035	NO	1034:1035	NO	1034:1035	The results demonstrated that DAPI staining, the comet assay, and DNA fragmentation showed that HWG prevented LPS-induced DNA damage in RAW264.7 macrophages, reduced the content of LPS-induced nitric oxide (NO) and malondialdehyde (MDA), increased GSH levels, and regulated antioxidant enzyme activities.
31152813	6	22	theme	LPS-induced	1008:1018	arg1	oxide					1027:1031	LPS-induced nitric oxide	1008:1031	LPS-induced nitric oxide (NO)	1008:1036	The results demonstrated that DAPI staining, the comet assay, and DNA fragmentation showed that HWG prevented LPS-induced DNA damage in RAW264.7 macrophages, reduced the content of LPS-induced nitric oxide (NO) and malondialdehyde (MDA), increased GSH levels, and regulated antioxidant enzyme activities.
31152813	6	23	theme	enzyme	1113:1118	arg1	activities					1120:1129	antioxidant enzyme activities	1101:1129	antioxidant enzyme activities	1101:1129	The results demonstrated that DAPI staining, the comet assay, and DNA fragmentation showed that HWG prevented LPS-induced DNA damage in RAW264.7 macrophages, reduced the content of LPS-induced nitric oxide (NO) and malondialdehyde (MDA), increased GSH levels, and regulated antioxidant enzyme activities.
31152813	4	24	theme	α-diphenyl-β-picrylhydrazyl	617:643	arg1	assays					677:682	the α,α-diphenyl-β-picrylhydrazyl (DPPH) and β-carotene bleaching assays	611:682	the α,α-diphenyl-β-picrylhydrazyl (DPPH) and β-carotene bleaching assays	611:682	The free radical scavenging capacity of HWG in cell-free systems was evaluated by using the α,α-diphenyl-β-picrylhydrazyl (DPPH) and β-carotene bleaching assays.
31152813	4	25	theme	α	615:615	arg1	assays					677:682	the α,α-diphenyl-β-picrylhydrazyl (DPPH) and β-carotene bleaching assays	611:682	the α,α-diphenyl-β-picrylhydrazyl (DPPH) and β-carotene bleaching assays	611:682	The free radical scavenging capacity of HWG in cell-free systems was evaluated by using the α,α-diphenyl-β-picrylhydrazyl (DPPH) and β-carotene bleaching assays.
31152813	0	26	theme	anti-inflammatory	90:106	arg1	activities					108:117	its antioxidant, and anti-inflammatory activities	69:117	its antioxidant, and anti-inflammatory activities	69:117	Phytochemical profile of hot water extract of Glechoma hederacea and its antioxidant, and anti-inflammatory activities.
31152813	6	27	theme	DAPI	857:860	arg1	staining					862:869	DAPI staining	857:869	DAPI staining	857:869	The results demonstrated that DAPI staining, the comet assay, and DNA fragmentation showed that HWG prevented LPS-induced DNA damage in RAW264.7 macrophages, reduced the content of LPS-induced nitric oxide (NO) and malondialdehyde (MDA), increased GSH levels, and regulated antioxidant enzyme activities.
31152813	3	28	theme	rosmarinic	394:403	arg1	acid					472:475	ferulic acid	464:475	ferulic acid	464:475	Our results indicated that rosmarinic acid, chlorogenic acid, caffeic acid, rutin, genistin, and ferulic acid were the most abundant phytochemicals in HWG.
31152813	3	28	theme	rosmarinic	394:403	arg1	acid					423:426	chlorogenic acid	411:426	chlorogenic acid	411:426	Our results indicated that rosmarinic acid, chlorogenic acid, caffeic acid, rutin, genistin, and ferulic acid were the most abundant phytochemicals in HWG.
31152813	3	28	theme	rosmarinic	394:403	arg1	genistin					450:457	genistin	450:457	genistin	450:457	Our results indicated that rosmarinic acid, chlorogenic acid, caffeic acid, rutin, genistin, and ferulic acid were the most abundant phytochemicals in HWG.
31152813	3	28	theme	rosmarinic	394:403	arg1	acid					405:408	rosmarinic acid	394:408	rosmarinic acid	394:408	Our results indicated that rosmarinic acid, chlorogenic acid, caffeic acid, rutin, genistin, and ferulic acid were the most abundant phytochemicals in HWG.
31152813	3	28	theme	rosmarinic	394:403	arg1	phytochemicals					500:513	the most abundant phytochemicals	482:513	the most abundant phytochemicals in HWG	482:520	Our results indicated that rosmarinic acid, chlorogenic acid, caffeic acid, rutin, genistin, and ferulic acid were the most abundant phytochemicals in HWG.
31152813	3	28	theme	rosmarinic	394:403	arg1	acid					437:440	caffeic acid	429:440	caffeic acid	429:440	Our results indicated that rosmarinic acid, chlorogenic acid, caffeic acid, rutin, genistin, and ferulic acid were the most abundant phytochemicals in HWG.
31152813	3	28	theme	rosmarinic	394:403	arg1	rutin					443:447	rutin	443:447	rutin	443:447	Our results indicated that rosmarinic acid, chlorogenic acid, caffeic acid, rutin, genistin, and ferulic acid were the most abundant phytochemicals in HWG.
31152813	3	29	theme	abundant	491:498	arg1	acid					472:475	ferulic acid	464:475	ferulic acid	464:475	Our results indicated that rosmarinic acid, chlorogenic acid, caffeic acid, rutin, genistin, and ferulic acid were the most abundant phytochemicals in HWG.
31152813	3	29	theme	abundant	491:498	arg1	acid					423:426	chlorogenic acid	411:426	chlorogenic acid	411:426	Our results indicated that rosmarinic acid, chlorogenic acid, caffeic acid, rutin, genistin, and ferulic acid were the most abundant phytochemicals in HWG.
31152813	3	29	theme	abundant	491:498	arg1	genistin					450:457	genistin	450:457	genistin	450:457	Our results indicated that rosmarinic acid, chlorogenic acid, caffeic acid, rutin, genistin, and ferulic acid were the most abundant phytochemicals in HWG.
31152813	3	29	theme	abundant	491:498	arg1	acid					405:408	rosmarinic acid	394:408	rosmarinic acid	394:408	Our results indicated that rosmarinic acid, chlorogenic acid, caffeic acid, rutin, genistin, and ferulic acid were the most abundant phytochemicals in HWG.
31152813	3	29	theme	abundant	491:498	arg1	phytochemicals					500:513	the most abundant phytochemicals	482:513	the most abundant phytochemicals in HWG	482:520	Our results indicated that rosmarinic acid, chlorogenic acid, caffeic acid, rutin, genistin, and ferulic acid were the most abundant phytochemicals in HWG.
31152813	3	29	theme	abundant	491:498	arg1	acid					437:440	caffeic acid	429:440	caffeic acid	429:440	Our results indicated that rosmarinic acid, chlorogenic acid, caffeic acid, rutin, genistin, and ferulic acid were the most abundant phytochemicals in HWG.
31152813	3	29	theme	abundant	491:498	arg1	rutin					443:447	rutin	443:447	rutin	443:447	Our results indicated that rosmarinic acid, chlorogenic acid, caffeic acid, rutin, genistin, and ferulic acid were the most abundant phytochemicals in HWG.
31152813	7	30	theme	RAW264.7	1260:1267	arg1	macrophages					1269:1279	RAW264.7 macrophages	1260:1279	RAW264.7 macrophages	1260:1279	We also demonstrated that HWG significantly decreased the LPS-induced mRNA expression of iNOS, COX-2, TNF-α, IL-6, and IL-1β in RAW264.7 macrophages, and reduced the LPS-induced protein expression of iNOS and COX-2 in RAW264.7 macrophages.
31152813	5	31	theme	lipopolysaccharide	769:786	arg1	macrophages					814:824	lipopolysaccharide (LPS)-activated RAW 264.7 macrophages	769:824	lipopolysaccharide (LPS)-activated RAW 264.7 macrophages	769:824	The antioxidant and anti-inflammatory activities of HWG were determined in vitro in lipopolysaccharide (LPS)-activated RAW 264.7 macrophages.
31152813	7	32	theme	RAW264.7	1350:1357	arg1	macrophages					1359:1369	RAW264.7 macrophages	1350:1369	RAW264.7 macrophages	1350:1369	We also demonstrated that HWG significantly decreased the LPS-induced mRNA expression of iNOS, COX-2, TNF-α, IL-6, and IL-1β in RAW264.7 macrophages, and reduced the LPS-induced protein expression of iNOS and COX-2 in RAW264.7 macrophages.
31152813	1	33	theme	many	178:181	arg1	activities					194:203	many biological activities	178:203	many biological activities	178:203	Glechoma hederacea belongs to the Labiatae family and has many biological activities.
31152813	0	34	theme	Phytochemical	0:12	arg1	profile					14:20	Phytochemical profile	0:20	Phytochemical profile of hot water extract of Glechoma hederacea and its antioxidant, and anti-inflammatory activities.	0:118	Phytochemical profile of hot water extract of Glechoma hederacea and its antioxidant, and anti-inflammatory activities.
31152813	6	35	theme	LPS-induced	937:947	arg1	damage					953:958	LPS-induced DNA damage	937:958	LPS-induced DNA damage in RAW264.7 macrophages	937:982	The results demonstrated that DAPI staining, the comet assay, and DNA fragmentation showed that HWG prevented LPS-induced DNA damage in RAW264.7 macrophages, reduced the content of LPS-induced nitric oxide (NO) and malondialdehyde (MDA), increased GSH levels, and regulated antioxidant enzyme activities.
31152813	7	36	theme	COX-2	1341:1345	arg1	expression					1318:1327	the LPS-induced protein expression	1294:1327	the LPS-induced protein expression of iNOS and COX-2 in RAW264.7 macrophages	1294:1369	We also demonstrated that HWG significantly decreased the LPS-induced mRNA expression of iNOS, COX-2, TNF-α, IL-6, and IL-1β in RAW264.7 macrophages, and reduced the LPS-induced protein expression of iNOS and COX-2 in RAW264.7 macrophages.
31152813	6	37	theme	RAW264.7	963:970	arg1	macrophages					972:982	RAW264.7 macrophages	963:982	RAW264.7 macrophages	963:982	The results demonstrated that DAPI staining, the comet assay, and DNA fragmentation showed that HWG prevented LPS-induced DNA damage in RAW264.7 macrophages, reduced the content of LPS-induced nitric oxide (NO) and malondialdehyde (MDA), increased GSH levels, and regulated antioxidant enzyme activities.
31152813	1	38	theme	biological	183:192	arg1	activities					194:203	many biological activities	178:203	many biological activities	178:203	Glechoma hederacea belongs to the Labiatae family and has many biological activities.
31152813	0	39	theme	hot	25:27	arg1	extract					35:41	hot water extract	25:41	hot water extract of Glechoma hederacea	25:63	Phytochemical profile of hot water extract of Glechoma hederacea and its antioxidant, and anti-inflammatory activities.
31152813	5	40	theme	-activated	793:802	arg1	macrophages					814:824	lipopolysaccharide (LPS)-activated RAW 264.7 macrophages	769:824	lipopolysaccharide (LPS)-activated RAW 264.7 macrophages	769:824	The antioxidant and anti-inflammatory activities of HWG were determined in vitro in lipopolysaccharide (LPS)-activated RAW 264.7 macrophages.
31152813	4	41	theme	free	527:530	arg1	scavenging					540:549	free radical scavenging	527:549	The free radical scavenging capacity of HWG in cell-free systems	523:586	The free radical scavenging capacity of HWG in cell-free systems was evaluated by using the α,α-diphenyl-β-picrylhydrazyl (DPPH) and β-carotene bleaching assays.
31152813	2	42	theme	anti-inflammatory	292:308	arg1	activities					310:319	anti-inflammatory activities	292:319	anti-inflammatory activities	292:319	The objective of this study was to evaluate the chemical composition, antioxidant and anti-inflammatory activities of a hot water extract of G. hederacea (HWG).
31152813	5	43	theme	RAW	804:806	arg1	macrophages					814:824	lipopolysaccharide (LPS)-activated RAW 264.7 macrophages	769:824	lipopolysaccharide (LPS)-activated RAW 264.7 macrophages	769:824	The antioxidant and anti-inflammatory activities of HWG were determined in vitro in lipopolysaccharide (LPS)-activated RAW 264.7 macrophages.
31152813	0	44	theme	extract	35:41	arg1	profile					14:20	Phytochemical profile	0:20	Phytochemical profile of hot water extract of Glechoma hederacea and its antioxidant, and anti-inflammatory activities.	0:118	Phytochemical profile of hot water extract of Glechoma hederacea and its antioxidant, and anti-inflammatory activities.
31152813	2	45	theme	extract	336:342	arg1	antioxidant					276:286	antioxidant	276:286	antioxidant	276:286	The objective of this study was to evaluate the chemical composition, antioxidant and anti-inflammatory activities of a hot water extract of G. hederacea (HWG).
31152813	2	45	theme	extract	336:342	arg1	activities					310:319	anti-inflammatory activities	292:319	anti-inflammatory activities	292:319	The objective of this study was to evaluate the chemical composition, antioxidant and anti-inflammatory activities of a hot water extract of G. hederacea (HWG).
31152813	2	45	theme	extract	336:342	arg1	composition					263:273	the chemical composition	250:273	the chemical composition	250:273	The objective of this study was to evaluate the chemical composition, antioxidant and anti-inflammatory activities of a hot water extract of G. hederacea (HWG).
31152813	7	46	theme	TNF-α	1234:1238	arg1	expression					1207:1216	the LPS-induced mRNA expression	1186:1216	the LPS-induced mRNA expression of iNOS, COX-2, TNF-α, IL-6, and IL-1β in RAW264.7 macrophages	1186:1279	We also demonstrated that HWG significantly decreased the LPS-induced mRNA expression of iNOS, COX-2, TNF-α, IL-6, and IL-1β in RAW264.7 macrophages, and reduced the LPS-induced protein expression of iNOS and COX-2 in RAW264.7 macrophages.
31152813	7	47	theme	protein	1310:1316	arg1	expression					1318:1327	the LPS-induced protein expression	1294:1327	the LPS-induced protein expression of iNOS and COX-2 in RAW264.7 macrophages	1294:1369	We also demonstrated that HWG significantly decreased the LPS-induced mRNA expression of iNOS, COX-2, TNF-α, IL-6, and IL-1β in RAW264.7 macrophages, and reduced the LPS-induced protein expression of iNOS and COX-2 in RAW264.7 macrophages.
31152813	3	48	theme	chlorogenic	411:421	arg1	acid					472:475	ferulic acid	464:475	ferulic acid	464:475	Our results indicated that rosmarinic acid, chlorogenic acid, caffeic acid, rutin, genistin, and ferulic acid were the most abundant phytochemicals in HWG.
31152813	3	48	theme	chlorogenic	411:421	arg1	acid					423:426	chlorogenic acid	411:426	chlorogenic acid	411:426	Our results indicated that rosmarinic acid, chlorogenic acid, caffeic acid, rutin, genistin, and ferulic acid were the most abundant phytochemicals in HWG.
31152813	3	48	theme	chlorogenic	411:421	arg1	genistin					450:457	genistin	450:457	genistin	450:457	Our results indicated that rosmarinic acid, chlorogenic acid, caffeic acid, rutin, genistin, and ferulic acid were the most abundant phytochemicals in HWG.
31152813	3	48	theme	chlorogenic	411:421	arg1	acid					405:408	rosmarinic acid	394:408	rosmarinic acid	394:408	Our results indicated that rosmarinic acid, chlorogenic acid, caffeic acid, rutin, genistin, and ferulic acid were the most abundant phytochemicals in HWG.
31152813	3	48	theme	chlorogenic	411:421	arg1	phytochemicals					500:513	the most abundant phytochemicals	482:513	the most abundant phytochemicals in HWG	482:520	Our results indicated that rosmarinic acid, chlorogenic acid, caffeic acid, rutin, genistin, and ferulic acid were the most abundant phytochemicals in HWG.
31152813	3	48	theme	chlorogenic	411:421	arg1	acid					437:440	caffeic acid	429:440	caffeic acid	429:440	Our results indicated that rosmarinic acid, chlorogenic acid, caffeic acid, rutin, genistin, and ferulic acid were the most abundant phytochemicals in HWG.
31152813	3	48	theme	chlorogenic	411:421	arg1	rutin					443:447	rutin	443:447	rutin	443:447	Our results indicated that rosmarinic acid, chlorogenic acid, caffeic acid, rutin, genistin, and ferulic acid were the most abundant phytochemicals in HWG.
31152813	3	49	theme	caffeic	429:435	arg1	acid					472:475	ferulic acid	464:475	ferulic acid	464:475	Our results indicated that rosmarinic acid, chlorogenic acid, caffeic acid, rutin, genistin, and ferulic acid were the most abundant phytochemicals in HWG.
31152813	3	49	theme	caffeic	429:435	arg1	acid					423:426	chlorogenic acid	411:426	chlorogenic acid	411:426	Our results indicated that rosmarinic acid, chlorogenic acid, caffeic acid, rutin, genistin, and ferulic acid were the most abundant phytochemicals in HWG.
31152813	3	49	theme	caffeic	429:435	arg1	genistin					450:457	genistin	450:457	genistin	450:457	Our results indicated that rosmarinic acid, chlorogenic acid, caffeic acid, rutin, genistin, and ferulic acid were the most abundant phytochemicals in HWG.
31152813	3	49	theme	caffeic	429:435	arg1	acid					405:408	rosmarinic acid	394:408	rosmarinic acid	394:408	Our results indicated that rosmarinic acid, chlorogenic acid, caffeic acid, rutin, genistin, and ferulic acid were the most abundant phytochemicals in HWG.
31152813	3	49	theme	caffeic	429:435	arg1	phytochemicals					500:513	the most abundant phytochemicals	482:513	the most abundant phytochemicals in HWG	482:520	Our results indicated that rosmarinic acid, chlorogenic acid, caffeic acid, rutin, genistin, and ferulic acid were the most abundant phytochemicals in HWG.
31152813	3	49	theme	caffeic	429:435	arg1	acid					437:440	caffeic acid	429:440	caffeic acid	429:440	Our results indicated that rosmarinic acid, chlorogenic acid, caffeic acid, rutin, genistin, and ferulic acid were the most abundant phytochemicals in HWG.
31152813	3	49	theme	caffeic	429:435	arg1	rutin					443:447	rutin	443:447	rutin	443:447	Our results indicated that rosmarinic acid, chlorogenic acid, caffeic acid, rutin, genistin, and ferulic acid were the most abundant phytochemicals in HWG.
31152813	0	50	theme	water	29:33	arg1	extract					35:41	hot water extract	25:41	hot water extract of Glechoma hederacea	25:63	Phytochemical profile of hot water extract of Glechoma hederacea and its antioxidant, and anti-inflammatory activities.
31152813	1	51	contain	has	174:176	arg1	hederacea					129:137	Glechoma hederacea	120:137	Glechoma hederacea	120:137	Glechoma hederacea belongs to the Labiatae family and has many biological activities.
31152813	1	51	contain	has	174:176	arg2	activities					194:203	many biological activities	178:203	many biological activities	178:203	Glechoma hederacea belongs to the Labiatae family and has many biological activities.
31152813	2	52	theme	study	228:232	arg1	objective					210:218	The objective	206:218	The objective of this study	206:232	The objective of this study was to evaluate the chemical composition, antioxidant and anti-inflammatory activities of a hot water extract of G. hederacea (HWG).
31152813	8	53	contain	possess	1424:1430	arg1	components					1413:1422	its main components	1404:1422	its main components	1404:1422	These results show that HWG and its main components possess potent antioxidant and anti-inflammatory properties.
31152813	8	53	contain	possess	1424:1430	arg2	properties					1473:1482	potent antioxidant and anti-inflammatory properties	1432:1482	potent antioxidant and anti-inflammatory properties	1432:1482	These results show that HWG and its main components possess potent antioxidant and anti-inflammatory properties.
31152813	8	53	contain	possess	1424:1430	arg1	HWG					1396:1398	HWG	1396:1398	HWG	1396:1398	These results show that HWG and its main components possess potent antioxidant and anti-inflammatory properties.
31152813	5	54	theme	anti-inflammatory	705:721	arg1	activities					723:732	The antioxidant and anti-inflammatory activities	685:732	The antioxidant and anti-inflammatory activities of HWG	685:739	The antioxidant and anti-inflammatory activities of HWG were determined in vitro in lipopolysaccharide (LPS)-activated RAW 264.7 macrophages.
31152813	0	55	theme	Glechoma	46:53	arg1	hederacea					55:63	Glechoma hederacea	46:63	Glechoma hederacea	46:63	Phytochemical profile of hot water extract of Glechoma hederacea and its antioxidant, and anti-inflammatory activities.
31152813	3	56	theme	ferulic	464:470	arg1	acid					472:475	ferulic acid	464:475	ferulic acid	464:475	Our results indicated that rosmarinic acid, chlorogenic acid, caffeic acid, rutin, genistin, and ferulic acid were the most abundant phytochemicals in HWG.
31152813	3	56	theme	ferulic	464:470	arg1	acid					423:426	chlorogenic acid	411:426	chlorogenic acid	411:426	Our results indicated that rosmarinic acid, chlorogenic acid, caffeic acid, rutin, genistin, and ferulic acid were the most abundant phytochemicals in HWG.
31152813	3	56	theme	ferulic	464:470	arg1	genistin					450:457	genistin	450:457	genistin	450:457	Our results indicated that rosmarinic acid, chlorogenic acid, caffeic acid, rutin, genistin, and ferulic acid were the most abundant phytochemicals in HWG.
31152813	3	56	theme	ferulic	464:470	arg1	acid					405:408	rosmarinic acid	394:408	rosmarinic acid	394:408	Our results indicated that rosmarinic acid, chlorogenic acid, caffeic acid, rutin, genistin, and ferulic acid were the most abundant phytochemicals in HWG.
31152813	3	56	theme	ferulic	464:470	arg1	phytochemicals					500:513	the most abundant phytochemicals	482:513	the most abundant phytochemicals in HWG	482:520	Our results indicated that rosmarinic acid, chlorogenic acid, caffeic acid, rutin, genistin, and ferulic acid were the most abundant phytochemicals in HWG.
31152813	3	56	theme	ferulic	464:470	arg1	acid					437:440	caffeic acid	429:440	caffeic acid	429:440	Our results indicated that rosmarinic acid, chlorogenic acid, caffeic acid, rutin, genistin, and ferulic acid were the most abundant phytochemicals in HWG.
31152813	3	56	theme	ferulic	464:470	arg1	rutin					443:447	rutin	443:447	rutin	443:447	Our results indicated that rosmarinic acid, chlorogenic acid, caffeic acid, rutin, genistin, and ferulic acid were the most abundant phytochemicals in HWG.
31152813	4	57	theme	bleaching	667:675	arg1	assays					677:682	the α,α-diphenyl-β-picrylhydrazyl (DPPH) and β-carotene bleaching assays	611:682	the α,α-diphenyl-β-picrylhydrazyl (DPPH) and β-carotene bleaching assays	611:682	The free radical scavenging capacity of HWG in cell-free systems was evaluated by using the α,α-diphenyl-β-picrylhydrazyl (DPPH) and β-carotene bleaching assays.
31152813	6	58	theme	DNA	893:895	arg1	fragmentation					897:909	DNA fragmentation	893:909	DNA fragmentation	893:909	The results demonstrated that DAPI staining, the comet assay, and DNA fragmentation showed that HWG prevented LPS-induced DNA damage in RAW264.7 macrophages, reduced the content of LPS-induced nitric oxide (NO) and malondialdehyde (MDA), increased GSH levels, and regulated antioxidant enzyme activities.
31152813	7	59	theme	IL-1β	1251:1255	arg1	expression					1207:1216	the LPS-induced mRNA expression	1186:1216	the LPS-induced mRNA expression of iNOS, COX-2, TNF-α, IL-6, and IL-1β in RAW264.7 macrophages	1186:1279	We also demonstrated that HWG significantly decreased the LPS-induced mRNA expression of iNOS, COX-2, TNF-α, IL-6, and IL-1β in RAW264.7 macrophages, and reduced the LPS-induced protein expression of iNOS and COX-2 in RAW264.7 macrophages.
31152813	7	60	theme	LPS-induced	1298:1308	arg1	expression					1318:1327	the LPS-induced protein expression	1294:1327	the LPS-induced protein expression of iNOS and COX-2 in RAW264.7 macrophages	1294:1369	We also demonstrated that HWG significantly decreased the LPS-induced mRNA expression of iNOS, COX-2, TNF-α, IL-6, and IL-1β in RAW264.7 macrophages, and reduced the LPS-induced protein expression of iNOS and COX-2 in RAW264.7 macrophages.
31152813	7	61	theme	COX-2	1227:1231	arg1	expression					1207:1216	the LPS-induced mRNA expression	1186:1216	the LPS-induced mRNA expression of iNOS, COX-2, TNF-α, IL-6, and IL-1β in RAW264.7 macrophages	1186:1279	We also demonstrated that HWG significantly decreased the LPS-induced mRNA expression of iNOS, COX-2, TNF-α, IL-6, and IL-1β in RAW264.7 macrophages, and reduced the LPS-induced protein expression of iNOS and COX-2 in RAW264.7 macrophages.
31152813	1	62	theme	Glechoma	120:127	arg1	hederacea					129:137	Glechoma hederacea	120:137	Glechoma hederacea	120:137	Glechoma hederacea belongs to the Labiatae family and has many biological activities.
31152813	2	63	theme	chemical	254:261	arg1	composition					263:273	the chemical composition	250:273	the chemical composition	250:273	The objective of this study was to evaluate the chemical composition, antioxidant and anti-inflammatory activities of a hot water extract of G. hederacea (HWG).
31152813	2	64	theme	water	330:334	arg1	extract					336:342	a hot water extract	324:342	a hot water extract	324:342	The objective of this study was to evaluate the chemical composition, antioxidant and anti-inflammatory activities of a hot water extract of G. hederacea (HWG).
31152813	6	65	theme	antioxidant	1101:1111	arg1	activities					1120:1129	antioxidant enzyme activities	1101:1129	antioxidant enzyme activities	1101:1129	The results demonstrated that DAPI staining, the comet assay, and DNA fragmentation showed that HWG prevented LPS-induced DNA damage in RAW264.7 macrophages, reduced the content of LPS-induced nitric oxide (NO) and malondialdehyde (MDA), increased GSH levels, and regulated antioxidant enzyme activities.
31152813	8	66	theme	main	1408:1411	arg1	components					1413:1422	its main components	1404:1422	its main components	1404:1422	These results show that HWG and its main components possess potent antioxidant and anti-inflammatory properties.
31152813	6	67	theme	oxide	1027:1031	arg1	content					997:1003	the content	993:1003	the content of LPS-induced nitric oxide (NO) and malondialdehyde (MDA)	993:1062	The results demonstrated that DAPI staining, the comet assay, and DNA fragmentation showed that HWG prevented LPS-induced DNA damage in RAW264.7 macrophages, reduced the content of LPS-induced nitric oxide (NO) and malondialdehyde (MDA), increased GSH levels, and regulated antioxidant enzyme activities.
31152813	2	68	theme	hot	326:328	arg1	extract					336:342	a hot water extract	324:342	a hot water extract	324:342	The objective of this study was to evaluate the chemical composition, antioxidant and anti-inflammatory activities of a hot water extract of G. hederacea (HWG).
31152813	4	69	theme	HWG	563:565	arg1	capacity					551:558	The free radical scavenging capacity	523:558	The free radical scavenging capacity of HWG in cell-free systems	523:586	The free radical scavenging capacity of HWG in cell-free systems was evaluated by using the α,α-diphenyl-β-picrylhydrazyl (DPPH) and β-carotene bleaching assays.
31152813	4	70	from	systems	580:586	arg1	capacity					551:558	The free radical scavenging capacity	523:558	The free radical scavenging capacity of HWG in cell-free systems	523:586	The free radical scavenging capacity of HWG in cell-free systems was evaluated by using the α,α-diphenyl-β-picrylhydrazyl (DPPH) and β-carotene bleaching assays.
31152813	5	71	theme	antioxidant	689:699	arg1	activities					723:732	The antioxidant and anti-inflammatory activities	685:732	The antioxidant and anti-inflammatory activities of HWG	685:739	The antioxidant and anti-inflammatory activities of HWG were determined in vitro in lipopolysaccharide (LPS)-activated RAW 264.7 macrophages.
31152813	6	72	theme	nitric	1020:1025	arg1	NO					1034:1035	NO	1034:1035	NO	1034:1035	The results demonstrated that DAPI staining, the comet assay, and DNA fragmentation showed that HWG prevented LPS-induced DNA damage in RAW264.7 macrophages, reduced the content of LPS-induced nitric oxide (NO) and malondialdehyde (MDA), increased GSH levels, and regulated antioxidant enzyme activities.
31152813	6	72	theme	nitric	1020:1025	arg1	oxide					1027:1031	LPS-induced nitric oxide	1008:1031	LPS-induced nitric oxide (NO)	1008:1036	The results demonstrated that DAPI staining, the comet assay, and DNA fragmentation showed that HWG prevented LPS-induced DNA damage in RAW264.7 macrophages, reduced the content of LPS-induced nitric oxide (NO) and malondialdehyde (MDA), increased GSH levels, and regulated antioxidant enzyme activities.
31152813	7	73	from	expression	1318:1327	arg1	macrophages					1359:1369	RAW264.7 macrophages	1350:1369	RAW264.7 macrophages	1350:1369	We also demonstrated that HWG significantly decreased the LPS-induced mRNA expression of iNOS, COX-2, TNF-α, IL-6, and IL-1β in RAW264.7 macrophages, and reduced the LPS-induced protein expression of iNOS and COX-2 in RAW264.7 macrophages.
31152813	7	74	theme	IL-6	1241:1244	arg1	expression					1207:1216	the LPS-induced mRNA expression	1186:1216	the LPS-induced mRNA expression of iNOS, COX-2, TNF-α, IL-6, and IL-1β in RAW264.7 macrophages	1186:1279	We also demonstrated that HWG significantly decreased the LPS-induced mRNA expression of iNOS, COX-2, TNF-α, IL-6, and IL-1β in RAW264.7 macrophages, and reduced the LPS-induced protein expression of iNOS and COX-2 in RAW264.7 macrophages.
31152813	0	75	theme	hederacea	55:63	arg1	extract					35:41	hot water extract	25:41	hot water extract of Glechoma hederacea	25:63	Phytochemical profile of hot water extract of Glechoma hederacea and its antioxidant, and anti-inflammatory activities.
31152813	0	75	theme	hederacea	55:63	arg1	activities					108:117	its antioxidant, and anti-inflammatory activities	69:117	its antioxidant, and anti-inflammatory activities	69:117	Phytochemical profile of hot water extract of Glechoma hederacea and its antioxidant, and anti-inflammatory activities.
31152813	4	76	theme	cell-free	570:578	arg1	systems					580:586	cell-free systems	570:586	cell-free systems	570:586	The free radical scavenging capacity of HWG in cell-free systems was evaluated by using the α,α-diphenyl-β-picrylhydrazyl (DPPH) and β-carotene bleaching assays.
31152813	7	77	theme	iNOS	1221:1224	arg1	expression					1207:1216	the LPS-induced mRNA expression	1186:1216	the LPS-induced mRNA expression of iNOS, COX-2, TNF-α, IL-6, and IL-1β in RAW264.7 macrophages	1186:1279	We also demonstrated that HWG significantly decreased the LPS-induced mRNA expression of iNOS, COX-2, TNF-α, IL-6, and IL-1β in RAW264.7 macrophages, and reduced the LPS-induced protein expression of iNOS and COX-2 in RAW264.7 macrophages.
31152813	7	78	theme	LPS-induced	1190:1200	arg1	expression					1207:1216	the LPS-induced mRNA expression	1186:1216	the LPS-induced mRNA expression of iNOS, COX-2, TNF-α, IL-6, and IL-1β in RAW264.7 macrophages	1186:1279	We also demonstrated that HWG significantly decreased the LPS-induced mRNA expression of iNOS, COX-2, TNF-α, IL-6, and IL-1β in RAW264.7 macrophages, and reduced the LPS-induced protein expression of iNOS and COX-2 in RAW264.7 macrophages.
31152813	4	79	theme	β-carotene	656:665	arg1	bleaching					667:675	β-carotene bleaching	656:675	β-carotene bleaching	656:675	The free radical scavenging capacity of HWG in cell-free systems was evaluated by using the α,α-diphenyl-β-picrylhydrazyl (DPPH) and β-carotene bleaching assays.
30513737	9	0	theme	nigericin	1710:1718	arg1	choice					1683:1688	the choice	1679:1688	the choice of activator ATP or nigericin	1679:1718	Moreover, this strong reduction of pro-inflammatory IL-1β is associated with a decrease of NLRP3 and, in J774A, ASC protein expression, which depends on the choice of activator ATP or nigericin.
30513737	1	1	theme	age-related	336:346	arg1	diseases					348:355	age-related diseases	336:355	age-related diseases	336:355	Inflammation has been described as an initiator event of major diseases with significant impacts in terms of public health including in cardiovascular disease, autoimmune disorders, eye diseases, age-related diseases, and the occurrence of cancers.
30513737	3	2	theme	pro-inflammatory	626:641	arg1	cytokines					643:651	pro-inflammatory cytokines	626:651	pro-inflammatory cytokines	626:651	Many studies have reported the value of polyphenols such as resveratrol in counteracting pro-inflammatory cytokines.
30513737	1	3	theme	public	249:254	arg1	health					256:261	public health	249:261	public health including in cardiovascular disease	249:297	Inflammation has been described as an initiator event of major diseases with significant impacts in terms of public health including in cardiovascular disease, autoimmune disorders, eye diseases, age-related diseases, and the occurrence of cancers.
30513737	2	4	theme	advantageous	473:484	arg1	approach					486:493	an advantageous approach	470:493	an advantageous approach to reducing these associated pathologies	470:534	A preventive action to reduce the key processes leading to inflammation could be an advantageous approach to reducing these associated pathologies.
30513737	2	4	theme	advantageous	473:484	arg1	action					402:407	A preventive action	389:407	A preventive action to reduce the key processes leading to inflammation	389:459	A preventive action to reduce the key processes leading to inflammation could be an advantageous approach to reducing these associated pathologies.
30513737	1	5	theme	diseases	348:355	arg1	event					188:192	an initiator event	175:192	an initiator event of major diseases with significant impacts in terms of public health including in cardiovascular disease, autoimmune disorders, eye diseases, age-related diseases, and the occurrence of cancers	175:386	Inflammation has been described as an initiator event of major diseases with significant impacts in terms of public health including in cardiovascular disease, autoimmune disorders, eye diseases, age-related diseases, and the occurrence of cancers.
30513737	1	5	theme	diseases	348:355	arg1	Inflammation					140:151	Inflammation	140:151	Inflammation	140:151	Inflammation has been described as an initiator event of major diseases with significant impacts in terms of public health including in cardiovascular disease, autoimmune disorders, eye diseases, age-related diseases, and the occurrence of cancers.
30513737	8	6	theme	β	1471:1471	arg1	secretion					1473:1481	IL-1 β secretion	1466:1481	IL-1 β secretion	1466:1481	Using two different macrophages, one of which does not express the adaptor protein ASC (apoptosis-associated speck-like protein containing a CARD), which is essential to form active inflammasome complexes that produce IL-1β, we show that RWE decreases IL-1 β secretion and gene expression whatever line is used.
30513737	8	7	theme	speck-like	1323:1332	arg1	protein					1334:1340	apoptosis-associated speck-like protein	1302:1340	apoptosis-associated speck-like protein containing a CARD	1302:1358	Using two different macrophages, one of which does not express the adaptor protein ASC (apoptosis-associated speck-like protein containing a CARD), which is essential to form active inflammasome complexes that produce IL-1β, we show that RWE decreases IL-1 β secretion and gene expression whatever line is used.
30513737	8	8	theme	gene	1487:1490	arg1	expression					1492:1501	gene expression	1487:1501	gene expression	1487:1501	Using two different macrophages, one of which does not express the adaptor protein ASC (apoptosis-associated speck-like protein containing a CARD), which is essential to form active inflammasome complexes that produce IL-1β, we show that RWE decreases IL-1 β secretion and gene expression whatever line is used.
30513737	1	9	theme	health	256:261	arg1	terms					240:244	terms	240:244	terms of public health including in cardiovascular disease	240:297	Inflammation has been described as an initiator event of major diseases with significant impacts in terms of public health including in cardiovascular disease, autoimmune disorders, eye diseases, age-related diseases, and the occurrence of cancers.
30513737	8	10	used	used	1520:1523	arg2	line					1512:1515	line	1512:1515	line	1512:1515	Using two different macrophages, one of which does not express the adaptor protein ASC (apoptosis-associated speck-like protein containing a CARD), which is essential to form active inflammasome complexes that produce IL-1β, we show that RWE decreases IL-1 β secretion and gene expression whatever line is used.
30513737	1	11	with	diseases	326:333	arg1	impacts					229:235	significant impacts	217:235	significant impacts in terms of public health including in cardiovascular disease	217:297	Inflammation has been described as an initiator event of major diseases with significant impacts in terms of public health including in cardiovascular disease, autoimmune disorders, eye diseases, age-related diseases, and the occurrence of cancers.
30513737	4	12	theme	polyphenolic	769:780	arg1	composition					782:792	its qualitative and quantitative polyphenolic composition	736:792	its qualitative and quantitative polyphenolic composition	736:792	We have previously shown the potential of red wine extract (RWE) and the value of its qualitative and quantitative polyphenolic composition to prevent the carcinogenesis process.
30513737	5	13	theme	inflammasome	951:962	arg1	pathway					964:970	the NLRP3 inflammasome pathway	941:970	the NLRP3 inflammasome pathway	941:970	In this study, we addressed a new effect of RWE in inflammation through a modulation of IL-1β secretion and the NLRP3 inflammasome pathway.
30513737	8	14	dep	protein	1289:1295	arg1	ASC					1297:1299	ASC	1297:1299	the adaptor protein ASC (apoptosis-associated speck-like protein containing a CARD)	1277:1359	Using two different macrophages, one of which does not express the adaptor protein ASC (apoptosis-associated speck-like protein containing a CARD), which is essential to form active inflammasome complexes that produce IL-1β, we show that RWE decreases IL-1 β secretion and gene expression whatever line is used.
30513737	8	14	dep	protein	1289:1295	arg1	protein					1334:1340	apoptosis-associated speck-like protein	1302:1340	apoptosis-associated speck-like protein containing a CARD	1302:1358	Using two different macrophages, one of which does not express the adaptor protein ASC (apoptosis-associated speck-like protein containing a CARD), which is essential to form active inflammasome complexes that produce IL-1β, we show that RWE decreases IL-1 β secretion and gene expression whatever line is used.
30513737	9	15	theme	pro-inflammatory	1561:1576	arg1	IL-1β					1578:1582	pro-inflammatory IL-1β	1561:1582	pro-inflammatory IL-1β	1561:1582	Moreover, this strong reduction of pro-inflammatory IL-1β is associated with a decrease of NLRP3 and, in J774A, ASC protein expression, which depends on the choice of activator ATP or nigericin.
30513737	7	16	theme	Inflammasome	1119:1130	arg1	formation					1132:1140	Inflammasome formation	1119:1140	Inflammasome formation	1119:1140	Inflammasome formation is triggered by a range of substances such as lipopolysaccharide (LPS).
30513737	1	17	with	disorders	311:319	arg1	impacts					229:235	significant impacts	217:235	significant impacts in terms of public health including in cardiovascular disease	217:297	Inflammation has been described as an initiator event of major diseases with significant impacts in terms of public health including in cardiovascular disease, autoimmune disorders, eye diseases, age-related diseases, and the occurrence of cancers.
30513737	4	18	theme	quantitative	756:767	arg1	composition					782:792	its qualitative and quantitative polyphenolic composition	736:792	its qualitative and quantitative polyphenolic composition	736:792	We have previously shown the potential of red wine extract (RWE) and the value of its qualitative and quantitative polyphenolic composition to prevent the carcinogenesis process.
30513737	5	19	theme	new	863:865	arg1	effect					867:872	a new effect	861:872	a new effect of RWE in inflammation	861:895	In this study, we addressed a new effect of RWE in inflammation through a modulation of IL-1β secretion and the NLRP3 inflammasome pathway.
30513737	0	20	theme	Interleukin-1-Beta	105:122	arg1	Modulation					91:100	a Modulation	89:100	a Modulation of Interleukin-1-Beta in Macrophages	89:137	Study of Potential Anti-Inflammatory Effects of Red Wine Extract and Resveratrol through a Modulation of Interleukin-1-Beta in Macrophages.
30513737	6	21	dep	priming	1014:1020	arg1	increase					1025:1032	increase	1025:1032	to increase the synthesis of NLRP3 and pro-IL-1β proteins	1022:1078	NLRP3 inflammasome requires two signals, priming to increase the synthesis of NLRP3 and pro-IL-1β proteins and activation, which activates NLRP3.
30513737	7	22	theme	substances	1169:1178	arg1	lipopolysaccharide					1188:1205	lipopolysaccharide	1188:1205	lipopolysaccharide (LPS)	1188:1211	Inflammasome formation is triggered by a range of substances such as lipopolysaccharide (LPS).
30513737	7	22	theme	substances	1169:1178	arg1	range					1160:1164	a range	1158:1164	a range of substances such as lipopolysaccharide (LPS)	1158:1211	Inflammasome formation is triggered by a range of substances such as lipopolysaccharide (LPS).
30513737	6	23	theme	NLRP3	1051:1055	arg1	synthesis					1038:1046	the synthesis	1034:1046	the synthesis of NLRP3 and pro-IL-1β proteins	1034:1078	NLRP3 inflammasome requires two signals, priming to increase the synthesis of NLRP3 and pro-IL-1β proteins and activation, which activates NLRP3.
30513737	3	24	theme	Many	537:540	arg1	studies					542:548	Many studies	537:548	Many studies	537:548	Many studies have reported the value of polyphenols such as resveratrol in counteracting pro-inflammatory cytokines.
30513737	6	25	theme	proteins	1071:1078	arg1	synthesis					1038:1046	the synthesis	1034:1046	the synthesis of NLRP3 and pro-IL-1β proteins	1034:1078	NLRP3 inflammasome requires two signals, priming to increase the synthesis of NLRP3 and pro-IL-1β proteins and activation, which activates NLRP3.
30513737	2	26	theme	preventive	391:400	arg1	approach					486:493	an advantageous approach	470:493	an advantageous approach to reducing these associated pathologies	470:534	A preventive action to reduce the key processes leading to inflammation could be an advantageous approach to reducing these associated pathologies.
30513737	2	26	theme	preventive	391:400	arg1	action					402:407	A preventive action	389:407	A preventive action to reduce the key processes leading to inflammation	389:459	A preventive action to reduce the key processes leading to inflammation could be an advantageous approach to reducing these associated pathologies.
30513737	1	27	theme	occurrence	366:375	arg1	event					188:192	an initiator event	175:192	an initiator event of major diseases with significant impacts in terms of public health including in cardiovascular disease, autoimmune disorders, eye diseases, age-related diseases, and the occurrence of cancers	175:386	Inflammation has been described as an initiator event of major diseases with significant impacts in terms of public health including in cardiovascular disease, autoimmune disorders, eye diseases, age-related diseases, and the occurrence of cancers.
30513737	1	27	theme	occurrence	366:375	arg1	Inflammation					140:151	Inflammation	140:151	Inflammation	140:151	Inflammation has been described as an initiator event of major diseases with significant impacts in terms of public health including in cardiovascular disease, autoimmune disorders, eye diseases, age-related diseases, and the occurrence of cancers.
30513737	9	28	theme	ASC	1638:1640	arg1	expression					1650:1659	ASC protein expression	1638:1659	ASC protein expression	1638:1659	Moreover, this strong reduction of pro-inflammatory IL-1β is associated with a decrease of NLRP3 and, in J774A, ASC protein expression, which depends on the choice of activator ATP or nigericin.
30513737	4	29	theme	composition	782:792	arg1	potential					683:691	the potential	679:691	the potential of red wine extract (RWE)	679:717	We have previously shown the potential of red wine extract (RWE) and the value of its qualitative and quantitative polyphenolic composition to prevent the carcinogenesis process.
30513737	4	29	theme	composition	782:792	arg1	value					727:731	the value	723:731	the value of its qualitative and quantitative polyphenolic composition	723:792	We have previously shown the potential of red wine extract (RWE) and the value of its qualitative and quantitative polyphenolic composition to prevent the carcinogenesis process.
30513737	1	30	theme	initiator	178:186	arg1	event					188:192	an initiator event	175:192	an initiator event of major diseases with significant impacts in terms of public health including in cardiovascular disease, autoimmune disorders, eye diseases, age-related diseases, and the occurrence of cancers	175:386	Inflammation has been described as an initiator event of major diseases with significant impacts in terms of public health including in cardiovascular disease, autoimmune disorders, eye diseases, age-related diseases, and the occurrence of cancers.
30513737	1	30	theme	initiator	178:186	arg1	Inflammation					140:151	Inflammation	140:151	Inflammation	140:151	Inflammation has been described as an initiator event of major diseases with significant impacts in terms of public health including in cardiovascular disease, autoimmune disorders, eye diseases, age-related diseases, and the occurrence of cancers.
30513737	9	31	theme	protein	1642:1648	arg1	expression					1650:1659	ASC protein expression	1638:1659	ASC protein expression	1638:1659	Moreover, this strong reduction of pro-inflammatory IL-1β is associated with a decrease of NLRP3 and, in J774A, ASC protein expression, which depends on the choice of activator ATP or nigericin.
30513737	9	32	theme	ATP	1703:1705	arg1	choice					1683:1688	the choice	1679:1688	the choice of activator ATP or nigericin	1679:1718	Moreover, this strong reduction of pro-inflammatory IL-1β is associated with a decrease of NLRP3 and, in J774A, ASC protein expression, which depends on the choice of activator ATP or nigericin.
30513737	9	33	theme	strong	1541:1546	arg1	reduction					1548:1556	this strong reduction	1536:1556	this strong reduction of pro-inflammatory IL-1β	1536:1582	Moreover, this strong reduction of pro-inflammatory IL-1β is associated with a decrease of NLRP3 and, in J774A, ASC protein expression, which depends on the choice of activator ATP or nigericin.
30513737	9	34	from	decrease	1605:1612	arg1	J774A					1631:1635	J774A	1631:1635	J774A	1631:1635	Moreover, this strong reduction of pro-inflammatory IL-1β is associated with a decrease of NLRP3 and, in J774A, ASC protein expression, which depends on the choice of activator ATP or nigericin.
30513737	4	35	theme	red	696:698	arg1	RWE					714:716	RWE	714:716	RWE	714:716	We have previously shown the potential of red wine extract (RWE) and the value of its qualitative and quantitative polyphenolic composition to prevent the carcinogenesis process.
30513737	4	35	theme	red	696:698	arg1	extract					705:711	red wine extract	696:711	red wine extract (RWE)	696:717	We have previously shown the potential of red wine extract (RWE) and the value of its qualitative and quantitative polyphenolic composition to prevent the carcinogenesis process.
30513737	1	36	theme	cardiovascular	276:289	arg1	disease					291:297	cardiovascular disease	276:297	cardiovascular disease	276:297	Inflammation has been described as an initiator event of major diseases with significant impacts in terms of public health including in cardiovascular disease, autoimmune disorders, eye diseases, age-related diseases, and the occurrence of cancers.
30513737	3	37	theme	polyphenols	577:587	arg1	value					568:572	the value	564:572	the value of polyphenols such as resveratrol	564:607	Many studies have reported the value of polyphenols such as resveratrol in counteracting pro-inflammatory cytokines.
30513737	5	38	from	effect	867:872	arg1	inflammation					884:895	inflammation	884:895	inflammation	884:895	In this study, we addressed a new effect of RWE in inflammation through a modulation of IL-1β secretion and the NLRP3 inflammasome pathway.
30513737	5	39	theme	RWE	877:879	arg1	effect					867:872	a new effect	861:872	a new effect of RWE in inflammation	861:895	In this study, we addressed a new effect of RWE in inflammation through a modulation of IL-1β secretion and the NLRP3 inflammasome pathway.
30513737	0	40	theme	Anti-Inflammatory	19:35	arg1	Effects					37:43	Potential Anti-Inflammatory Effects	9:43	Potential Anti-Inflammatory Effects of Red Wine Extract	9:63	Study of Potential Anti-Inflammatory Effects of Red Wine Extract and Resveratrol through a Modulation of Interleukin-1-Beta in Macrophages.
30513737	6	41	theme	NLRP3	973:977	arg1	inflammasome					979:990	NLRP3 inflammasome	973:990	NLRP3 inflammasome	973:990	NLRP3 inflammasome requires two signals, priming to increase the synthesis of NLRP3 and pro-IL-1β proteins and activation, which activates NLRP3.
30513737	5	42	theme	NLRP3	945:949	arg1	pathway					964:970	the NLRP3 inflammasome pathway	941:970	the NLRP3 inflammasome pathway	941:970	In this study, we addressed a new effect of RWE in inflammation through a modulation of IL-1β secretion and the NLRP3 inflammasome pathway.
30513737	9	43	theme	IL-1β	1578:1582	arg1	reduction					1548:1556	this strong reduction	1536:1556	this strong reduction of pro-inflammatory IL-1β	1536:1582	Moreover, this strong reduction of pro-inflammatory IL-1β is associated with a decrease of NLRP3 and, in J774A, ASC protein expression, which depends on the choice of activator ATP or nigericin.
30513737	1	44	theme	major	197:201	arg1	diseases					203:210	major diseases	197:210	major diseases with significant impacts in terms of public health including in cardiovascular disease	197:297	Inflammation has been described as an initiator event of major diseases with significant impacts in terms of public health including in cardiovascular disease, autoimmune disorders, eye diseases, age-related diseases, and the occurrence of cancers.
30513737	0	45	theme	Potential	9:17	arg1	Effects					37:43	Potential Anti-Inflammatory Effects	9:43	Potential Anti-Inflammatory Effects of Red Wine Extract	9:63	Study of Potential Anti-Inflammatory Effects of Red Wine Extract and Resveratrol through a Modulation of Interleukin-1-Beta in Macrophages.
30513737	1	46	theme	cancers	380:386	arg1	diseases					348:355	age-related diseases	336:355	age-related diseases	336:355	Inflammation has been described as an initiator event of major diseases with significant impacts in terms of public health including in cardiovascular disease, autoimmune disorders, eye diseases, age-related diseases, and the occurrence of cancers.
30513737	1	46	theme	cancers	380:386	arg1	diseases					203:210	major diseases	197:210	major diseases with significant impacts in terms of public health including in cardiovascular disease	197:297	Inflammation has been described as an initiator event of major diseases with significant impacts in terms of public health including in cardiovascular disease, autoimmune disorders, eye diseases, age-related diseases, and the occurrence of cancers.
30513737	1	46	theme	cancers	380:386	arg1	occurrence					366:375	the occurrence	362:375	the occurrence of cancers	362:386	Inflammation has been described as an initiator event of major diseases with significant impacts in terms of public health including in cardiovascular disease, autoimmune disorders, eye diseases, age-related diseases, and the occurrence of cancers.
30513737	1	46	theme	cancers	380:386	arg1	disorders					311:319	autoimmune disorders	300:319	autoimmune disorders	300:319	Inflammation has been described as an initiator event of major diseases with significant impacts in terms of public health including in cardiovascular disease, autoimmune disorders, eye diseases, age-related diseases, and the occurrence of cancers.
30513737	1	46	theme	cancers	380:386	arg1	diseases					326:333	eye diseases	322:333	eye diseases	322:333	Inflammation has been described as an initiator event of major diseases with significant impacts in terms of public health including in cardiovascular disease, autoimmune disorders, eye diseases, age-related diseases, and the occurrence of cancers.
30513737	4	47	theme	qualitative	740:750	arg1	composition					782:792	its qualitative and quantitative polyphenolic composition	736:792	its qualitative and quantitative polyphenolic composition	736:792	We have previously shown the potential of red wine extract (RWE) and the value of its qualitative and quantitative polyphenolic composition to prevent the carcinogenesis process.
30513737	1	48	theme	diseases	203:210	arg1	event					188:192	an initiator event	175:192	an initiator event of major diseases with significant impacts in terms of public health including in cardiovascular disease, autoimmune disorders, eye diseases, age-related diseases, and the occurrence of cancers	175:386	Inflammation has been described as an initiator event of major diseases with significant impacts in terms of public health including in cardiovascular disease, autoimmune disorders, eye diseases, age-related diseases, and the occurrence of cancers.
30513737	1	48	theme	diseases	203:210	arg1	Inflammation					140:151	Inflammation	140:151	Inflammation	140:151	Inflammation has been described as an initiator event of major diseases with significant impacts in terms of public health including in cardiovascular disease, autoimmune disorders, eye diseases, age-related diseases, and the occurrence of cancers.
30513737	1	49	theme	autoimmune	300:309	arg1	disorders					311:319	autoimmune disorders	300:319	autoimmune disorders	300:319	Inflammation has been described as an initiator event of major diseases with significant impacts in terms of public health including in cardiovascular disease, autoimmune disorders, eye diseases, age-related diseases, and the occurrence of cancers.
30513737	4	50	theme	carcinogenesis	809:822	arg1	process					824:830	the carcinogenesis process	805:830	the carcinogenesis process	805:830	We have previously shown the potential of red wine extract (RWE) and the value of its qualitative and quantitative polyphenolic composition to prevent the carcinogenesis process.
30513737	6	51	theme	pro-IL-1β	1061:1069	arg1	proteins					1071:1078	pro-IL-1β proteins	1061:1078	pro-IL-1β proteins	1061:1078	NLRP3 inflammasome requires two signals, priming to increase the synthesis of NLRP3 and pro-IL-1β proteins and activation, which activates NLRP3.
30513737	0	52	theme	Effects	37:43	arg1	Resveratrol					69:79	Resveratrol	69:79	Resveratrol through a Modulation of Interleukin-1-Beta in Macrophages	69:137	Study of Potential Anti-Inflammatory Effects of Red Wine Extract and Resveratrol through a Modulation of Interleukin-1-Beta in Macrophages.
30513737	0	52	theme	Effects	37:43	arg1	Study					0:4	Study	0:4	Study of Potential Anti-Inflammatory Effects of Red Wine Extract	0:63	Study of Potential Anti-Inflammatory Effects of Red Wine Extract and Resveratrol through a Modulation of Interleukin-1-Beta in Macrophages.
30513737	1	53	theme	disorders	311:319	arg1	event					188:192	an initiator event	175:192	an initiator event of major diseases with significant impacts in terms of public health including in cardiovascular disease, autoimmune disorders, eye diseases, age-related diseases, and the occurrence of cancers	175:386	Inflammation has been described as an initiator event of major diseases with significant impacts in terms of public health including in cardiovascular disease, autoimmune disorders, eye diseases, age-related diseases, and the occurrence of cancers.
30513737	1	53	theme	disorders	311:319	arg1	Inflammation					140:151	Inflammation	140:151	Inflammation	140:151	Inflammation has been described as an initiator event of major diseases with significant impacts in terms of public health including in cardiovascular disease, autoimmune disorders, eye diseases, age-related diseases, and the occurrence of cancers.
30513737	1	54	with	diseases	203:210	arg1	impacts					229:235	significant impacts	217:235	significant impacts in terms of public health including in cardiovascular disease	217:297	Inflammation has been described as an initiator event of major diseases with significant impacts in terms of public health including in cardiovascular disease, autoimmune disorders, eye diseases, age-related diseases, and the occurrence of cancers.
30513737	8	55	contain	containing	1342:1351	arg2	CARD					1355:1358	a CARD	1353:1358	a CARD	1353:1358	Using two different macrophages, one of which does not express the adaptor protein ASC (apoptosis-associated speck-like protein containing a CARD), which is essential to form active inflammasome complexes that produce IL-1β, we show that RWE decreases IL-1 β secretion and gene expression whatever line is used.
30513737	8	55	contain	containing	1342:1351	arg1	protein					1334:1340	apoptosis-associated speck-like protein	1302:1340	apoptosis-associated speck-like protein containing a CARD	1302:1358	Using two different macrophages, one of which does not express the adaptor protein ASC (apoptosis-associated speck-like protein containing a CARD), which is essential to form active inflammasome complexes that produce IL-1β, we show that RWE decreases IL-1 β secretion and gene expression whatever line is used.
30513737	1	56	theme	significant	217:227	arg1	impacts					229:235	significant impacts	217:235	significant impacts in terms of public health including in cardiovascular disease	217:297	Inflammation has been described as an initiator event of major diseases with significant impacts in terms of public health including in cardiovascular disease, autoimmune disorders, eye diseases, age-related diseases, and the occurrence of cancers.
30513737	0	57	theme	Wine	52:55	arg1	Extract					57:63	Red Wine Extract	48:63	Red Wine Extract	48:63	Study of Potential Anti-Inflammatory Effects of Red Wine Extract and Resveratrol through a Modulation of Interleukin-1-Beta in Macrophages.
30513737	8	58	theme	inflammasome	1396:1407	arg1	complexes					1409:1417	active inflammasome complexes	1389:1417	active inflammasome complexes that produce IL-1β	1389:1436	Using two different macrophages, one of which does not express the adaptor protein ASC (apoptosis-associated speck-like protein containing a CARD), which is essential to form active inflammasome complexes that produce IL-1β, we show that RWE decreases IL-1 β secretion and gene expression whatever line is used.
30513737	8	59	theme	adaptor	1281:1287	arg1	protein					1289:1295	the adaptor protein	1277:1295	the adaptor protein ASC (apoptosis-associated speck-like protein containing a CARD)	1277:1359	Using two different macrophages, one of which does not express the adaptor protein ASC (apoptosis-associated speck-like protein containing a CARD), which is essential to form active inflammasome complexes that produce IL-1β, we show that RWE decreases IL-1 β secretion and gene expression whatever line is used.
30513737	8	59	theme	adaptor	1281:1287	arg1	essential					1371:1379	essential	1371:1379	essential	1371:1379	Using two different macrophages, one of which does not express the adaptor protein ASC (apoptosis-associated speck-like protein containing a CARD), which is essential to form active inflammasome complexes that produce IL-1β, we show that RWE decreases IL-1 β secretion and gene expression whatever line is used.
30513737	0	60	theme	Red	48:50	arg1	Extract					57:63	Red Wine Extract	48:63	Red Wine Extract	48:63	Study of Potential Anti-Inflammatory Effects of Red Wine Extract and Resveratrol through a Modulation of Interleukin-1-Beta in Macrophages.
30513737	1	61	theme	eye	322:324	arg1	diseases					326:333	eye diseases	322:333	eye diseases	322:333	Inflammation has been described as an initiator event of major diseases with significant impacts in terms of public health including in cardiovascular disease, autoimmune disorders, eye diseases, age-related diseases, and the occurrence of cancers.
30513737	0	62	from	Interleukin-1-Beta	105:122	arg1	Macrophages					127:137	Macrophages	127:137	Macrophages	127:137	Study of Potential Anti-Inflammatory Effects of Red Wine Extract and Resveratrol through a Modulation of Interleukin-1-Beta in Macrophages.
30513737	8	63	theme	active	1389:1394	arg1	complexes					1409:1417	active inflammasome complexes	1389:1417	active inflammasome complexes that produce IL-1β	1389:1436	Using two different macrophages, one of which does not express the adaptor protein ASC (apoptosis-associated speck-like protein containing a CARD), which is essential to form active inflammasome complexes that produce IL-1β, we show that RWE decreases IL-1 β secretion and gene expression whatever line is used.
30513737	1	64	with	occurrence	366:375	arg1	impacts					229:235	significant impacts	217:235	significant impacts in terms of public health including in cardiovascular disease	217:297	Inflammation has been described as an initiator event of major diseases with significant impacts in terms of public health including in cardiovascular disease, autoimmune disorders, eye diseases, age-related diseases, and the occurrence of cancers.
30513737	1	65	theme	diseases	326:333	arg1	event					188:192	an initiator event	175:192	an initiator event of major diseases with significant impacts in terms of public health including in cardiovascular disease, autoimmune disorders, eye diseases, age-related diseases, and the occurrence of cancers	175:386	Inflammation has been described as an initiator event of major diseases with significant impacts in terms of public health including in cardiovascular disease, autoimmune disorders, eye diseases, age-related diseases, and the occurrence of cancers.
30513737	1	65	theme	diseases	326:333	arg1	Inflammation					140:151	Inflammation	140:151	Inflammation	140:151	Inflammation has been described as an initiator event of major diseases with significant impacts in terms of public health including in cardiovascular disease, autoimmune disorders, eye diseases, age-related diseases, and the occurrence of cancers.
30513737	1	66	from	impacts	229:235	arg1	terms					240:244	terms	240:244	terms of public health including in cardiovascular disease	240:297	Inflammation has been described as an initiator event of major diseases with significant impacts in terms of public health including in cardiovascular disease, autoimmune disorders, eye diseases, age-related diseases, and the occurrence of cancers.
30513737	9	67	theme	NLRP3	1617:1621	arg1	decrease					1605:1612	a decrease	1603:1612	a decrease	1603:1612	Moreover, this strong reduction of pro-inflammatory IL-1β is associated with a decrease of NLRP3 and, in J774A, ASC protein expression, which depends on the choice of activator ATP or nigericin.
30513737	5	68	theme	IL-1β	921:925	arg1	secretion					927:935	IL-1β secretion	921:935	IL-1β secretion	921:935	In this study, we addressed a new effect of RWE in inflammation through a modulation of IL-1β secretion and the NLRP3 inflammasome pathway.
30513737	9	69	theme	activator	1693:1701	arg1	ATP					1703:1705	activator ATP	1693:1705	activator ATP	1693:1705	Moreover, this strong reduction of pro-inflammatory IL-1β is associated with a decrease of NLRP3 and, in J774A, ASC protein expression, which depends on the choice of activator ATP or nigericin.
30513737	0	70	from	Modulation	91:100	arg1	Macrophages					127:137	Macrophages	127:137	Macrophages	127:137	Study of Potential Anti-Inflammatory Effects of Red Wine Extract and Resveratrol through a Modulation of Interleukin-1-Beta in Macrophages.
30513737	8	71	theme	apoptosis-associated	1302:1321	arg1	protein					1334:1340	apoptosis-associated speck-like protein	1302:1340	apoptosis-associated speck-like protein containing a CARD	1302:1358	Using two different macrophages, one of which does not express the adaptor protein ASC (apoptosis-associated speck-like protein containing a CARD), which is essential to form active inflammasome complexes that produce IL-1β, we show that RWE decreases IL-1 β secretion and gene expression whatever line is used.
30513737	5	72	theme	secretion	927:935	arg1	pathway					964:970	the NLRP3 inflammasome pathway	941:970	the NLRP3 inflammasome pathway	941:970	In this study, we addressed a new effect of RWE in inflammation through a modulation of IL-1β secretion and the NLRP3 inflammasome pathway.
30513737	5	72	theme	secretion	927:935	arg1	modulation					907:916	a modulation	905:916	a modulation of IL-1β secretion	905:935	In this study, we addressed a new effect of RWE in inflammation through a modulation of IL-1β secretion and the NLRP3 inflammasome pathway.
30513737	8	73	theme	different	1224:1232	arg1	macrophages					1234:1244	two different macrophages	1220:1244	two different macrophages	1220:1244	Using two different macrophages, one of which does not express the adaptor protein ASC (apoptosis-associated speck-like protein containing a CARD), which is essential to form active inflammasome complexes that produce IL-1β, we show that RWE decreases IL-1 β secretion and gene expression whatever line is used.
30513737	2	74	theme	associated	513:522	arg1	pathologies					524:534	these associated pathologies	507:534	these associated pathologies	507:534	A preventive action to reduce the key processes leading to inflammation could be an advantageous approach to reducing these associated pathologies.
30513737	8	75	theme	IL-1	1466:1469	arg1	secretion					1473:1481	IL-1 β secretion	1466:1481	IL-1 β secretion	1466:1481	Using two different macrophages, one of which does not express the adaptor protein ASC (apoptosis-associated speck-like protein containing a CARD), which is essential to form active inflammasome complexes that produce IL-1β, we show that RWE decreases IL-1 β secretion and gene expression whatever line is used.
30513737	4	76	theme	extract	705:711	arg1	potential					683:691	the potential	679:691	the potential of red wine extract (RWE)	679:717	We have previously shown the potential of red wine extract (RWE) and the value of its qualitative and quantitative polyphenolic composition to prevent the carcinogenesis process.
30513737	4	76	theme	extract	705:711	arg1	value					727:731	the value	723:731	the value of its qualitative and quantitative polyphenolic composition	723:792	We have previously shown the potential of red wine extract (RWE) and the value of its qualitative and quantitative polyphenolic composition to prevent the carcinogenesis process.
30513737	8	77	theme	macrophages	1234:1244	arg1	one					1247:1249	one	1247:1249	one	1247:1249	Using two different macrophages, one of which does not express the adaptor protein ASC (apoptosis-associated speck-like protein containing a CARD), which is essential to form active inflammasome complexes that produce IL-1β, we show that RWE decreases IL-1 β secretion and gene expression whatever line is used.
30513737	8	77	theme	macrophages	1234:1244	arg1	macrophages					1234:1244	two different macrophages	1220:1244	two different macrophages	1220:1244	Using two different macrophages, one of which does not express the adaptor protein ASC (apoptosis-associated speck-like protein containing a CARD), which is essential to form active inflammasome complexes that produce IL-1β, we show that RWE decreases IL-1 β secretion and gene expression whatever line is used.
30513737	0	78	from	Macrophages	127:137	arg1	Modulation					91:100	a Modulation	89:100	a Modulation of Interleukin-1-Beta in Macrophages	89:137	Study of Potential Anti-Inflammatory Effects of Red Wine Extract and Resveratrol through a Modulation of Interleukin-1-Beta in Macrophages.
30513737	0	79	theme	Extract	57:63	arg1	Effects					37:43	Potential Anti-Inflammatory Effects	9:43	Potential Anti-Inflammatory Effects of Red Wine Extract	9:63	Study of Potential Anti-Inflammatory Effects of Red Wine Extract and Resveratrol through a Modulation of Interleukin-1-Beta in Macrophages.
30513737	2	80	theme	key	423:425	arg1	processes					427:435	the key processes	419:435	the key processes leading to inflammation	419:459	A preventive action to reduce the key processes leading to inflammation could be an advantageous approach to reducing these associated pathologies.
30513737	4	81	theme	wine	700:703	arg1	RWE					714:716	RWE	714:716	RWE	714:716	We have previously shown the potential of red wine extract (RWE) and the value of its qualitative and quantitative polyphenolic composition to prevent the carcinogenesis process.
30513737	4	81	theme	wine	700:703	arg1	extract					705:711	red wine extract	696:711	red wine extract (RWE)	696:717	We have previously shown the potential of red wine extract (RWE) and the value of its qualitative and quantitative polyphenolic composition to prevent the carcinogenesis process.
30513737	1	82	with	diseases	348:355	arg1	impacts					229:235	significant impacts	217:235	significant impacts in terms of public health including in cardiovascular disease	217:297	Inflammation has been described as an initiator event of major diseases with significant impacts in terms of public health including in cardiovascular disease, autoimmune disorders, eye diseases, age-related diseases, and the occurrence of cancers.
30142460	20	0	theme	rabbit	3067:3072	arg1	cells					3074:3078	the rabbit cells	3063:3078	the rabbit cells	3063:3078	This study also showed that the human cells transplanted into the rabbit discs did not induce a higher immune response than the rabbit cells.
30142460	16	1	theme	collagen	2263:2270	arg1	type					2289:2292	collagen type II/collagen type I	2263:2294	collagen type II/collagen type I	2263:2294	The ratio of collagen type II/collagen type I was higher in the IVDs treated with nHDFs (1.26) than those treated with RDFs (0.81) or saline (0.59) and intact discs (1.00).
30142460	2	2	theme	allogeneic	370:379	arg1	cells					398:402	autologous or allogeneic mesenchymal stem cells	356:402	autologous or allogeneic mesenchymal stem cells	356:402	Clinical studies utilizing autologous or allogeneic mesenchymal stem cells to treat patients with back pain have reported some encouraging results.
30142460	15	3	theme	treated	2235:2241	arg1	discs					2243:2247	saline treated discs	2228:2247	saline treated discs	2228:2247	Compared to intact discs, expression of both collagen types I and II increased significantly in nHDF-treated discs (p<.05), trending to significant in RDF-treated discs, and not significantly in saline treated discs.
30142460	2	4	with	patients	413:420	arg1	pain					432:435	back pain	427:435	back pain	427:435	Clinical studies utilizing autologous or allogeneic mesenchymal stem cells to treat patients with back pain have reported some encouraging results.
30142460	17	5	theme	RDF-treated	2555:2565	arg1	discs					2567:2571	the RDF-treated discs	2551:2571	the RDF-treated discs	2551:2571	Last, proteoglycan contents increased significantly in discs treated with nHDF (p<.05) and were trending toward significance in the RDF-treated discs compared to those treated with saline.
30142460	16	6	theme	II/collagen	2277:2287	arg1	type					2289:2292	collagen type II/collagen type I	2263:2294	collagen type II/collagen type I	2263:2294	The ratio of collagen type II/collagen type I was higher in the IVDs treated with nHDFs (1.26) than those treated with RDFs (0.81) or saline (0.59) and intact discs (1.00).
30142460	6	7	theme	degeneration	906:917	arg1	model					919:923	the rabbit disc degeneration model	890:923	the rabbit disc degeneration model	890:923	STUDY DESIGN Using the rabbit disc degeneration model, we compared transplantation of neonatal human dermal fibroblasts (nHDFs) and rabbit dermal fibroblasts (RDFs) into rabbit degenerated discs on host immune response, disc height, and IVD composition.
30142460	15	8	from	discs	2196:2200	arg1	significant					2169:2179	significant	2169:2179	significant	2169:2179	Compared to intact discs, expression of both collagen types I and II increased significantly in nHDF-treated discs (p<.05), trending to significant in RDF-treated discs, and not significantly in saline treated discs.
30142460	21	9	theme	allogeneic	3156:3165	arg1	grafts					3181:3186	allogeneic or xenogeneic grafts	3156:3186	allogeneic or xenogeneic grafts	3156:3186	These results support that the IVD is immune privileged and would tolerate allogeneic or xenogeneic grafts.
30142460	0	10	theme	Therapeutic	0:10	arg1	effects					12:18	Therapeutic effects	0:18	Therapeutic effects of cell therapy with neonatal human dermal fibroblasts and rabbit dermal fibroblasts on disc degeneration and inflammation	0:141	Therapeutic effects of cell therapy with neonatal human dermal fibroblasts and rabbit dermal fibroblasts on disc degeneration and inflammation.
30142460	6	11	theme	rabbit	894:899	arg1	model					919:923	the rabbit disc degeneration model	890:923	the rabbit disc degeneration model	890:923	STUDY DESIGN Using the rabbit disc degeneration model, we compared transplantation of neonatal human dermal fibroblasts (nHDFs) and rabbit dermal fibroblasts (RDFs) into rabbit degenerated discs on host immune response, disc height, and IVD composition.
30142460	21	12	theme	xenogeneic	3170:3179	arg1	grafts					3181:3186	allogeneic or xenogeneic grafts	3156:3186	allogeneic or xenogeneic grafts	3156:3186	These results support that the IVD is immune privileged and would tolerate allogeneic or xenogeneic grafts.
30142460	11	13	theme	proteoglycan	1557:1568	arg1	contents					1570:1577	proteoglycan contents	1557:1577	proteoglycan contents	1557:1577	IVDs were isolated for inflammatory and collagen gene expression analysis using real-time polymerase chain reaction and biochemical analysis of proteoglycan contents using dimethylmethylene blue assay.
30142460	13	14	dep	RESULTS	1694:1700	arg1	weeks					1708:1712	Eight weeks	1702:1712	Eight weeks after treatment	1702:1728	RESULTS Eight weeks after treatment, disc height indexes of discs treated with nHDF increased significantly by 7.8% (p<.01), whereas those treated with saline or RDF increased by 1.5% and 2.0%, respectively.
30142460	13	14	dep	RESULTS	1694:1700	arg1	indexes					1743:1749	disc height indexes	1731:1749	disc height indexes of discs treated with nHDF	1731:1776	RESULTS Eight weeks after treatment, disc height indexes of discs treated with nHDF increased significantly by 7.8% (p<.01), whereas those treated with saline or RDF increased by 1.5% and 2.0%, respectively.
30142460	7	15	dep	METHODS	1125:1131	arg1	received					1159:1166	received	1159:1166	received an annular puncture using an 18-guage needle to induce disc degeneration	1159:1239	METHODS New Zealand white rabbits received an annular puncture using an 18-guage needle to induce disc degeneration.
30142460	5	16	theme	study	725:729	arg1	PURPOSE					695:701	PURPOSE The objective	695:715	PURPOSE The objective of this study	695:729	PURPOSE The objective of this study is to determine the impact of donor source on the therapeutic effects of dermal fibroblast treatment on disc degeneration and inflammation.
30142460	5	17	from	impact	751:756	arg1	effects					793:799	the therapeutic effects	777:799	the therapeutic effects of dermal fibroblast treatment on disc degeneration and inflammation	777:868	PURPOSE The objective of this study is to determine the impact of donor source on the therapeutic effects of dermal fibroblast treatment on disc degeneration and inflammation.
30142460	6	18	theme	fibroblasts	979:989	arg1	transplantation					938:952	transplantation	938:952	transplantation of neonatal human dermal fibroblasts (nHDFs) and rabbit dermal fibroblasts (RDFs) into rabbit degenerated discs on host immune response, disc height, and IVD composition	938:1122	STUDY DESIGN Using the rabbit disc degeneration model, we compared transplantation of neonatal human dermal fibroblasts (nHDFs) and rabbit dermal fibroblasts (RDFs) into rabbit degenerated discs on host immune response, disc height, and IVD composition.
30142460	1	19	theme	intervertebral	241:254	arg1	IVD					262:264	IVD	262:264	IVD	262:264	BACKGROUND CONTEXT Increasing evidence suggests transplanting viable cells into the degenerating intervertebral disc (IVD) may be effective in treating disc degeneration and back pain.
30142460	1	19	theme	intervertebral	241:254	arg1	disc					256:259	the degenerating intervertebral disc	224:259	the degenerating intervertebral disc (IVD)	224:265	BACKGROUND CONTEXT Increasing evidence suggests transplanting viable cells into the degenerating intervertebral disc (IVD) may be effective in treating disc degeneration and back pain.
30142460	6	20	theme	human	966:970	arg1	nHDFs					992:996	nHDFs	992:996	nHDFs	992:996	STUDY DESIGN Using the rabbit disc degeneration model, we compared transplantation of neonatal human dermal fibroblasts (nHDFs) and rabbit dermal fibroblasts (RDFs) into rabbit degenerated discs on host immune response, disc height, and IVD composition.
30142460	6	20	theme	human	966:970	arg1	fibroblasts					979:989	neonatal human dermal fibroblasts	957:989	neonatal human dermal fibroblasts (nHDFs)	957:997	STUDY DESIGN Using the rabbit disc degeneration model, we compared transplantation of neonatal human dermal fibroblasts (nHDFs) and rabbit dermal fibroblasts (RDFs) into rabbit degenerated discs on host immune response, disc height, and IVD composition.
30142460	15	21	from	discs	2243:2247	arg1	significant					2169:2179	significant	2169:2179	significant	2169:2179	Compared to intact discs, expression of both collagen types I and II increased significantly in nHDF-treated discs (p<.05), trending to significant in RDF-treated discs, and not significantly in saline treated discs.
30142460	13	22	theme	height	1736:1741	arg1	weeks					1708:1712	Eight weeks	1702:1712	Eight weeks after treatment	1702:1728	RESULTS Eight weeks after treatment, disc height indexes of discs treated with nHDF increased significantly by 7.8% (p<.01), whereas those treated with saline or RDF increased by 1.5% and 2.0%, respectively.
30142460	13	22	theme	height	1736:1741	arg1	indexes					1743:1749	disc height indexes	1731:1749	disc height indexes of discs treated with nHDF	1731:1776	RESULTS Eight weeks after treatment, disc height indexes of discs treated with nHDF increased significantly by 7.8% (p<.01), whereas those treated with saline or RDF increased by 1.5% and 2.0%, respectively.
30142460	18	23	theme	gene	2793:2796	arg1	expression					2798:2807	II gene expression	2790:2807	II gene expression	2790:2807	CONCLUSIONS This study showed that cell transplantation with nHDF into degenerated IVDs can significantly increase markers of disc regeneration (disc height, collagen type I and II gene expression, and proteoglycan contents).
30142460	17	24	theme	proteoglycan	2429:2440	arg1	contents					2442:2449	proteoglycan contents	2429:2449	proteoglycan contents	2429:2449	Last, proteoglycan contents increased significantly in discs treated with nHDF (p<.05) and were trending toward significance in the RDF-treated discs compared to those treated with saline.
30142460	7	25	theme	Zealand	1137:1143	arg1	rabbits					1151:1157	New Zealand white rabbits	1133:1157	New Zealand white rabbits	1133:1157	METHODS New Zealand white rabbits received an annular puncture using an 18-guage needle to induce disc degeneration.
30142460	8	26	theme	5 × 106	1297:1303	arg1	nHDFs					1305:1309	5 × 106 nHDFs	1297:1309	5 × 106 nHDFs	1297:1309	Four weeks after injury, rabbit IVDs were treated with 5 × 106 nHDFs, RDFs, or saline.
30142460	16	27	dep	treated	2319:2325	arg1	IVDs					2314:2317	the IVDs	2310:2317	the IVDs treated with nHDFs (1.26)	2310:2343	The ratio of collagen type II/collagen type I was higher in the IVDs treated with nHDFs (1.26) than those treated with RDFs (0.81) or saline (0.59) and intact discs (1.00).
30142460	16	27	dep	treated	2319:2325	arg1	1.26					2339:2342	1.26	2339:2342	1.26	2339:2342	The ratio of collagen type II/collagen type I was higher in the IVDs treated with nHDFs (1.26) than those treated with RDFs (0.81) or saline (0.59) and intact discs (1.00).
30142460	20	28	theme	human	2971:2975	arg1	cells					2977:2981	the human cells	2967:2981	the human cells transplanted into the rabbit discs	2967:3016	This study also showed that the human cells transplanted into the rabbit discs did not induce a higher immune response than the rabbit cells.
30142460	15	29	dep	types	2087:2091	arg1	types					2087:2091	both collagen types I and II	2073:2100	both collagen types I and II	2073:2100	Compared to intact discs, expression of both collagen types I and II increased significantly in nHDF-treated discs (p<.05), trending to significant in RDF-treated discs, and not significantly in saline treated discs.
30142460	15	29	dep	types	2087:2091	arg1	II					2099:2100	II	2099:2100	II	2099:2100	Compared to intact discs, expression of both collagen types I and II increased significantly in nHDF-treated discs (p<.05), trending to significant in RDF-treated discs, and not significantly in saline treated discs.
30142460	15	29	dep	types	2087:2091	arg1	I					2093:2093	I	2093:2093	I	2093:2093	Compared to intact discs, expression of both collagen types I and II increased significantly in nHDF-treated discs (p<.05), trending to significant in RDF-treated discs, and not significantly in saline treated discs.
30142460	11	30	theme	expression	1467:1476	arg1	analysis					1478:1485	inflammatory and collagen gene expression analysis	1436:1485	inflammatory and collagen gene expression analysis using real-time polymerase chain reaction and biochemical analysis of proteoglycan contents	1436:1577	IVDs were isolated for inflammatory and collagen gene expression analysis using real-time polymerase chain reaction and biochemical analysis of proteoglycan contents using dimethylmethylene blue assay.
30142460	5	31	theme	source	767:772	arg1	impact					751:756	the impact	747:756	the impact of donor source on the therapeutic effects of dermal fibroblast treatment on disc degeneration and inflammation	747:868	PURPOSE The objective of this study is to determine the impact of donor source on the therapeutic effects of dermal fibroblast treatment on disc degeneration and inflammation.
30142460	1	32	theme	CONTEXT	155:161	arg1	evidence					174:181	BACKGROUND CONTEXT Increasing evidence	144:181	BACKGROUND CONTEXT Increasing evidence	144:181	BACKGROUND CONTEXT Increasing evidence suggests transplanting viable cells into the degenerating intervertebral disc (IVD) may be effective in treating disc degeneration and back pain.
30142460	17	33	from	trending	2519:2526	arg1	discs					2567:2571	the RDF-treated discs	2551:2571	the RDF-treated discs	2551:2571	Last, proteoglycan contents increased significantly in discs treated with nHDF (p<.05) and were trending toward significance in the RDF-treated discs compared to those treated with saline.
30142460	15	34	theme	nHDF-treated	2129:2140	arg1	discs					2142:2146	nHDF-treated discs	2129:2146	nHDF-treated discs	2129:2146	Compared to intact discs, expression of both collagen types I and II increased significantly in nHDF-treated discs (p<.05), trending to significant in RDF-treated discs, and not significantly in saline treated discs.
30142460	18	35	dep	CONCLUSIONS	2612:2622	arg1	showed					2635:2640	showed	2635:2640	showed that cell transplantation with nHDF into degenerated IVDs can significantly increase markers of disc regeneration (disc height, collagen type I and II gene expression, and proteoglycan contents)	2635:2835	CONCLUSIONS This study showed that cell transplantation with nHDF into degenerated IVDs can significantly increase markers of disc regeneration (disc height, collagen type I and II gene expression, and proteoglycan contents).
30142460	6	36	theme	dermal	1010:1015	arg1	RDFs					1030:1033	RDFs	1030:1033	RDFs	1030:1033	STUDY DESIGN Using the rabbit disc degeneration model, we compared transplantation of neonatal human dermal fibroblasts (nHDFs) and rabbit dermal fibroblasts (RDFs) into rabbit degenerated discs on host immune response, disc height, and IVD composition.
30142460	6	36	theme	dermal	1010:1015	arg1	fibroblasts					1017:1027	rabbit dermal fibroblasts	1003:1027	rabbit dermal fibroblasts (RDFs)	1003:1034	STUDY DESIGN Using the rabbit disc degeneration model, we compared transplantation of neonatal human dermal fibroblasts (nHDFs) and rabbit dermal fibroblasts (RDFs) into rabbit degenerated discs on host immune response, disc height, and IVD composition.
30142460	11	37	theme	polymerase	1503:1512	arg1	reaction					1520:1527	real-time polymerase chain reaction	1493:1527	real-time polymerase chain reaction	1493:1527	IVDs were isolated for inflammatory and collagen gene expression analysis using real-time polymerase chain reaction and biochemical analysis of proteoglycan contents using dimethylmethylene blue assay.
30142460	0	38	theme	dermal	86:91	arg1	fibroblasts					93:103	rabbit dermal fibroblasts	79:103	rabbit dermal fibroblasts	79:103	Therapeutic effects of cell therapy with neonatal human dermal fibroblasts and rabbit dermal fibroblasts on disc degeneration and inflammation.
30142460	4	39	dep	Studies	591:597	arg1	determine					602:610	determine	602:610	to determine the advantages and disadvantages of cell types and sources for therapy	599:681	Studies to determine the advantages and disadvantages of cell types and sources for therapy are needed.
30142460	5	40	dep	PURPOSE	695:701	arg1	objective					707:715	The objective	703:715	PURPOSE The objective of this study	695:729	PURPOSE The objective of this study is to determine the impact of donor source on the therapeutic effects of dermal fibroblast treatment on disc degeneration and inflammation.
30142460	18	41	theme	regeneration	2743:2754	arg1	markers					2727:2733	markers	2727:2733	markers of disc regeneration	2727:2754	CONCLUSIONS This study showed that cell transplantation with nHDF into degenerated IVDs can significantly increase markers of disc regeneration (disc height, collagen type I and II gene expression, and proteoglycan contents).
30142460	5	42	theme	therapeutic	781:791	arg1	effects					793:799	the therapeutic effects	777:799	the therapeutic effects of dermal fibroblast treatment on disc degeneration and inflammation	777:868	PURPOSE The objective of this study is to determine the impact of donor source on the therapeutic effects of dermal fibroblast treatment on disc degeneration and inflammation.
30142460	18	43	with	transplantation	2652:2666	arg1	nHDF					2673:2676	nHDF	2673:2676	nHDF	2673:2676	CONCLUSIONS This study showed that cell transplantation with nHDF into degenerated IVDs can significantly increase markers of disc regeneration (disc height, collagen type I and II gene expression, and proteoglycan contents).
30142460	5	44	theme	donor	761:765	arg1	source					767:772	donor source	761:772	donor source	761:772	PURPOSE The objective of this study is to determine the impact of donor source on the therapeutic effects of dermal fibroblast treatment on disc degeneration and inflammation.
30142460	20	45	theme	immune	3042:3047	arg1	response					3049:3056	a higher immune response	3033:3056	a higher immune response than the rabbit cells	3033:3078	This study also showed that the human cells transplanted into the rabbit discs did not induce a higher immune response than the rabbit cells.
30142460	5	46	theme	treatment	822:830	arg1	effects					793:799	the therapeutic effects	777:799	the therapeutic effects of dermal fibroblast treatment on disc degeneration and inflammation	777:868	PURPOSE The objective of this study is to determine the impact of donor source on the therapeutic effects of dermal fibroblast treatment on disc degeneration and inflammation.
30142460	8	47	dep	weeks	1247:1251	arg1	injury					1259:1264	injury	1259:1264	injury	1259:1264	Four weeks after injury, rabbit IVDs were treated with 5 × 106 nHDFs, RDFs, or saline.
30142460	12	48	theme	research	1646:1653	arg1	grant					1655:1659	a research grant	1644:1659	a research grant from SpinalCyte, LLC ($414,431)	1644:1691	These studies were funded by a research grant from SpinalCyte, LLC ($414,431).
30142460	6	49	theme	host	1069:1072	arg1	response					1081:1088	host immune response	1069:1088	host immune response	1069:1088	STUDY DESIGN Using the rabbit disc degeneration model, we compared transplantation of neonatal human dermal fibroblasts (nHDFs) and rabbit dermal fibroblasts (RDFs) into rabbit degenerated discs on host immune response, disc height, and IVD composition.
30142460	2	50	theme	encouraging	456:466	arg1	results					468:474	some encouraging results	451:474	some encouraging results	451:474	Clinical studies utilizing autologous or allogeneic mesenchymal stem cells to treat patients with back pain have reported some encouraging results.
30142460	16	51	theme	type	2272:2275	arg1	type					2289:2292	collagen type II/collagen type I	2263:2294	collagen type II/collagen type I	2263:2294	The ratio of collagen type II/collagen type I was higher in the IVDs treated with nHDFs (1.26) than those treated with RDFs (0.81) or saline (0.59) and intact discs (1.00).
30142460	11	52	theme	collagen	1453:1460	arg1	expression					1467:1476	collagen gene expression	1453:1476	collagen gene expression	1453:1476	IVDs were isolated for inflammatory and collagen gene expression analysis using real-time polymerase chain reaction and biochemical analysis of proteoglycan contents using dimethylmethylene blue assay.
30142460	5	53	theme	disc	835:838	arg1	degeneration					840:851	disc degeneration	835:851	disc degeneration	835:851	PURPOSE The objective of this study is to determine the impact of donor source on the therapeutic effects of dermal fibroblast treatment on disc degeneration and inflammation.
30142460	11	54	theme	inflammatory	1436:1447	arg1	analysis					1478:1485	inflammatory and collagen gene expression analysis	1436:1485	inflammatory and collagen gene expression analysis using real-time polymerase chain reaction and biochemical analysis of proteoglycan contents	1436:1577	IVDs were isolated for inflammatory and collagen gene expression analysis using real-time polymerase chain reaction and biochemical analysis of proteoglycan contents using dimethylmethylene blue assay.
30142460	3	55	theme	Animal	477:482	arg1	studies					484:490	Animal studies	477:490	Animal studies	477:490	Animal studies have shown that cells injected into the disc can survive for months and have regenerative effects.
30142460	0	56	theme	therapy	28:34	arg1	effects					12:18	Therapeutic effects	0:18	Therapeutic effects of cell therapy with neonatal human dermal fibroblasts and rabbit dermal fibroblasts on disc degeneration and inflammation	0:141	Therapeutic effects of cell therapy with neonatal human dermal fibroblasts and rabbit dermal fibroblasts on disc degeneration and inflammation.
30142460	20	57	theme	higher	3035:3040	arg1	response					3049:3056	a higher immune response	3033:3056	a higher immune response than the rabbit cells	3033:3078	This study also showed that the human cells transplanted into the rabbit discs did not induce a higher immune response than the rabbit cells.
30142460	6	58	theme	rabbit	1041:1046	arg1	discs					1060:1064	rabbit degenerated discs	1041:1064	rabbit degenerated discs on host immune response, disc height, and IVD composition	1041:1122	STUDY DESIGN Using the rabbit disc degeneration model, we compared transplantation of neonatal human dermal fibroblasts (nHDFs) and rabbit dermal fibroblasts (RDFs) into rabbit degenerated discs on host immune response, disc height, and IVD composition.
30142460	0	59	theme	dermal	56:61	arg1	fibroblasts					63:73	neonatal human dermal fibroblasts	41:73	neonatal human dermal fibroblasts	41:73	Therapeutic effects of cell therapy with neonatal human dermal fibroblasts and rabbit dermal fibroblasts on disc degeneration and inflammation.
30142460	5	60	theme	dermal	804:809	arg1	treatment					822:830	dermal fibroblast treatment	804:830	dermal fibroblast treatment	804:830	PURPOSE The objective of this study is to determine the impact of donor source on the therapeutic effects of dermal fibroblast treatment on disc degeneration and inflammation.
30142460	9	61	theme	post-treatment	1344:1357	arg1	weeks					1338:1342	eight weeks post-treatment	1332:1357	eight weeks post-treatment	1332:1357	At eight weeks post-treatment, animals were sacrificed.
30142460	15	62	dep	significantly	2211:2223	arg1	not					2207:2209	not	2207:2209	not	2207:2209	Compared to intact discs, expression of both collagen types I and II increased significantly in nHDF-treated discs (p<.05), trending to significant in RDF-treated discs, and not significantly in saline treated discs.
30142460	14	63	theme	Gene	1902:1905	arg1	analysis					1918:1925	Gene expression analysis	1902:1925	Gene expression analysis	1902:1925	Gene expression analysis showed that discs transplanted with nHDFs and RDFs displayed similar inflammatory responses (p=.2 to .8).
30142460	14	64	theme	inflammatory	1996:2007	arg1	responses					2009:2017	similar inflammatory responses	1988:2017	similar inflammatory responses	1988:2017	Gene expression analysis showed that discs transplanted with nHDFs and RDFs displayed similar inflammatory responses (p=.2 to .8).
30142460	18	65	theme	cell	2647:2650	arg1	transplantation					2652:2666	cell transplantation	2647:2666	cell transplantation with nHDF into degenerated IVDs	2647:2698	CONCLUSIONS This study showed that cell transplantation with nHDF into degenerated IVDs can significantly increase markers of disc regeneration (disc height, collagen type I and II gene expression, and proteoglycan contents).
30142460	18	66	theme	proteoglycan	2814:2825	arg1	contents					2827:2834	proteoglycan contents	2814:2834	proteoglycan contents	2814:2834	CONCLUSIONS This study showed that cell transplantation with nHDF into degenerated IVDs can significantly increase markers of disc regeneration (disc height, collagen type I and II gene expression, and proteoglycan contents).
30142460	16	67	theme	intact	2402:2407	arg1	discs					2409:2413	intact discs	2402:2413	intact discs	2402:2413	The ratio of collagen type II/collagen type I was higher in the IVDs treated with nHDFs (1.26) than those treated with RDFs (0.81) or saline (0.59) and intact discs (1.00).
30142460	15	68	theme	intact	2045:2050	arg1	discs					2052:2056	intact discs	2045:2056	intact discs	2045:2056	Compared to intact discs, expression of both collagen types I and II increased significantly in nHDF-treated discs (p<.05), trending to significant in RDF-treated discs, and not significantly in saline treated discs.
30142460	6	69	theme	IVD	1108:1110	arg1	composition					1112:1122	IVD composition	1108:1122	IVD composition	1108:1122	STUDY DESIGN Using the rabbit disc degeneration model, we compared transplantation of neonatal human dermal fibroblasts (nHDFs) and rabbit dermal fibroblasts (RDFs) into rabbit degenerated discs on host immune response, disc height, and IVD composition.
30142460	0	70	theme	human	50:54	arg1	fibroblasts					63:73	neonatal human dermal fibroblasts	41:73	neonatal human dermal fibroblasts	41:73	Therapeutic effects of cell therapy with neonatal human dermal fibroblasts and rabbit dermal fibroblasts on disc degeneration and inflammation.
30142460	0	71	with	therapy	28:34	arg1	fibroblasts					93:103	rabbit dermal fibroblasts	79:103	rabbit dermal fibroblasts	79:103	Therapeutic effects of cell therapy with neonatal human dermal fibroblasts and rabbit dermal fibroblasts on disc degeneration and inflammation.
30142460	0	71	with	therapy	28:34	arg1	fibroblasts					63:73	neonatal human dermal fibroblasts	41:73	neonatal human dermal fibroblasts	41:73	Therapeutic effects of cell therapy with neonatal human dermal fibroblasts and rabbit dermal fibroblasts on disc degeneration and inflammation.
30142460	2	72	theme	mesenchymal	381:391	arg1	cells					398:402	autologous or allogeneic mesenchymal stem cells	356:402	autologous or allogeneic mesenchymal stem cells	356:402	Clinical studies utilizing autologous or allogeneic mesenchymal stem cells to treat patients with back pain have reported some encouraging results.
30142460	15	73	theme	saline	2228:2233	arg1	discs					2243:2247	saline treated discs	2228:2247	saline treated discs	2228:2247	Compared to intact discs, expression of both collagen types I and II increased significantly in nHDF-treated discs (p<.05), trending to significant in RDF-treated discs, and not significantly in saline treated discs.
30142460	15	74	from	significant	2169:2179	arg1	discs					2243:2247	saline treated discs	2228:2247	saline treated discs	2228:2247	Compared to intact discs, expression of both collagen types I and II increased significantly in nHDF-treated discs (p<.05), trending to significant in RDF-treated discs, and not significantly in saline treated discs.
30142460	15	74	from	significant	2169:2179	arg1	discs					2196:2200	RDF-treated discs	2184:2200	RDF-treated discs	2184:2200	Compared to intact discs, expression of both collagen types I and II increased significantly in nHDF-treated discs (p<.05), trending to significant in RDF-treated discs, and not significantly in saline treated discs.
30142460	6	75	theme	disc	1091:1094	arg1	height					1096:1101	disc height	1091:1101	disc height	1091:1101	STUDY DESIGN Using the rabbit disc degeneration model, we compared transplantation of neonatal human dermal fibroblasts (nHDFs) and rabbit dermal fibroblasts (RDFs) into rabbit degenerated discs on host immune response, disc height, and IVD composition.
30142460	16	76	theme	type	2289:2292	arg1	higher					2300:2305	higher	2300:2305	higher	2300:2305	The ratio of collagen type II/collagen type I was higher in the IVDs treated with nHDFs (1.26) than those treated with RDFs (0.81) or saline (0.59) and intact discs (1.00).
30142460	16	76	theme	type	2289:2292	arg1	ratio					2254:2258	The ratio	2250:2258	The ratio of collagen type II/collagen type I	2250:2294	The ratio of collagen type II/collagen type I was higher in the IVDs treated with nHDFs (1.26) than those treated with RDFs (0.81) or saline (0.59) and intact discs (1.00).
30142460	11	77	theme	biochemical	1533:1543	arg1	analysis					1545:1552	biochemical analysis	1533:1552	biochemical analysis of proteoglycan contents	1533:1577	IVDs were isolated for inflammatory and collagen gene expression analysis using real-time polymerase chain reaction and biochemical analysis of proteoglycan contents using dimethylmethylene blue assay.
30142460	0	78	theme	neonatal	41:48	arg1	fibroblasts					63:73	neonatal human dermal fibroblasts	41:73	neonatal human dermal fibroblasts	41:73	Therapeutic effects of cell therapy with neonatal human dermal fibroblasts and rabbit dermal fibroblasts on disc degeneration and inflammation.
30142460	6	79	theme	disc	901:904	arg1	model					919:923	the rabbit disc degeneration model	890:923	the rabbit disc degeneration model	890:923	STUDY DESIGN Using the rabbit disc degeneration model, we compared transplantation of neonatal human dermal fibroblasts (nHDFs) and rabbit dermal fibroblasts (RDFs) into rabbit degenerated discs on host immune response, disc height, and IVD composition.
30142460	12	80	from	SpinalCyte	1666:1675	arg1	grant					1655:1659	a research grant	1644:1659	a research grant from SpinalCyte, LLC ($414,431)	1644:1691	These studies were funded by a research grant from SpinalCyte, LLC ($414,431).
30142460	11	81	theme	dimethylmethylene	1585:1601	arg1	assay					1608:1612	dimethylmethylene blue assay	1585:1612	dimethylmethylene blue assay	1585:1612	IVDs were isolated for inflammatory and collagen gene expression analysis using real-time polymerase chain reaction and biochemical analysis of proteoglycan contents using dimethylmethylene blue assay.
30142460	2	82	theme	Clinical	329:336	arg1	studies					338:344	Clinical studies	329:344	Clinical studies utilizing autologous or allogeneic mesenchymal stem cells to treat patients with back pain	329:435	Clinical studies utilizing autologous or allogeneic mesenchymal stem cells to treat patients with back pain have reported some encouraging results.
30142460	1	83	theme	viable	206:211	arg1	cells					213:217	viable cells	206:217	viable cells	206:217	BACKGROUND CONTEXT Increasing evidence suggests transplanting viable cells into the degenerating intervertebral disc (IVD) may be effective in treating disc degeneration and back pain.
30142460	20	84	theme	rabbit	3005:3010	arg1	discs					3012:3016	the rabbit discs	3001:3016	the rabbit discs	3001:3016	This study also showed that the human cells transplanted into the rabbit discs did not induce a higher immune response than the rabbit cells.
30142460	13	85	theme	discs	1754:1758	arg1	weeks					1708:1712	Eight weeks	1702:1712	Eight weeks after treatment	1702:1728	RESULTS Eight weeks after treatment, disc height indexes of discs treated with nHDF increased significantly by 7.8% (p<.01), whereas those treated with saline or RDF increased by 1.5% and 2.0%, respectively.
30142460	13	85	theme	discs	1754:1758	arg1	indexes					1743:1749	disc height indexes	1731:1749	disc height indexes of discs treated with nHDF	1731:1776	RESULTS Eight weeks after treatment, disc height indexes of discs treated with nHDF increased significantly by 7.8% (p<.01), whereas those treated with saline or RDF increased by 1.5% and 2.0%, respectively.
30142460	6	86	from	discs	1060:1064	arg1	composition					1112:1122	IVD composition	1108:1122	IVD composition	1108:1122	STUDY DESIGN Using the rabbit disc degeneration model, we compared transplantation of neonatal human dermal fibroblasts (nHDFs) and rabbit dermal fibroblasts (RDFs) into rabbit degenerated discs on host immune response, disc height, and IVD composition.
30142460	6	86	from	discs	1060:1064	arg1	response					1081:1088	host immune response	1069:1088	host immune response	1069:1088	STUDY DESIGN Using the rabbit disc degeneration model, we compared transplantation of neonatal human dermal fibroblasts (nHDFs) and rabbit dermal fibroblasts (RDFs) into rabbit degenerated discs on host immune response, disc height, and IVD composition.
30142460	6	86	from	discs	1060:1064	arg1	height					1096:1101	disc height	1091:1101	disc height	1091:1101	STUDY DESIGN Using the rabbit disc degeneration model, we compared transplantation of neonatal human dermal fibroblasts (nHDFs) and rabbit dermal fibroblasts (RDFs) into rabbit degenerated discs on host immune response, disc height, and IVD composition.
30142460	3	87	theme	regenerative	569:580	arg1	effects					582:588	regenerative effects	569:588	regenerative effects	569:588	Animal studies have shown that cells injected into the disc can survive for months and have regenerative effects.
30142460	15	88	theme	types	2087:2091	arg1	expression					2059:2068	expression	2059:2068	expression of both collagen types I and II	2059:2100	Compared to intact discs, expression of both collagen types I and II increased significantly in nHDF-treated discs (p<.05), trending to significant in RDF-treated discs, and not significantly in saline treated discs.
30142460	4	89	theme	types	653:657	arg1	disadvantages					631:643	disadvantages	631:643	disadvantages	631:643	Studies to determine the advantages and disadvantages of cell types and sources for therapy are needed.
30142460	4	89	theme	types	653:657	arg1	advantages					616:625	advantages	616:625	advantages	616:625	Studies to determine the advantages and disadvantages of cell types and sources for therapy are needed.
30142460	18	90	theme	collagen	2770:2777	arg1	type					2779:2782	collagen type I	2770:2784	collagen type I	2770:2784	CONCLUSIONS This study showed that cell transplantation with nHDF into degenerated IVDs can significantly increase markers of disc regeneration (disc height, collagen type I and II gene expression, and proteoglycan contents).
30142460	1	91	theme	degenerating	228:239	arg1	IVD					262:264	IVD	262:264	IVD	262:264	BACKGROUND CONTEXT Increasing evidence suggests transplanting viable cells into the degenerating intervertebral disc (IVD) may be effective in treating disc degeneration and back pain.
30142460	1	91	theme	degenerating	228:239	arg1	disc					256:259	the degenerating intervertebral disc	224:259	the degenerating intervertebral disc (IVD)	224:265	BACKGROUND CONTEXT Increasing evidence suggests transplanting viable cells into the degenerating intervertebral disc (IVD) may be effective in treating disc degeneration and back pain.
30142460	6	92	theme	dermal	972:977	arg1	nHDFs					992:996	nHDFs	992:996	nHDFs	992:996	STUDY DESIGN Using the rabbit disc degeneration model, we compared transplantation of neonatal human dermal fibroblasts (nHDFs) and rabbit dermal fibroblasts (RDFs) into rabbit degenerated discs on host immune response, disc height, and IVD composition.
30142460	6	92	theme	dermal	972:977	arg1	fibroblasts					979:989	neonatal human dermal fibroblasts	957:989	neonatal human dermal fibroblasts (nHDFs)	957:997	STUDY DESIGN Using the rabbit disc degeneration model, we compared transplantation of neonatal human dermal fibroblasts (nHDFs) and rabbit dermal fibroblasts (RDFs) into rabbit degenerated discs on host immune response, disc height, and IVD composition.
30142460	15	93	theme	collagen	2078:2085	arg1	types					2087:2091	both collagen types I and II	2073:2100	both collagen types I and II	2073:2100	Compared to intact discs, expression of both collagen types I and II increased significantly in nHDF-treated discs (p<.05), trending to significant in RDF-treated discs, and not significantly in saline treated discs.
30142460	15	93	theme	collagen	2078:2085	arg1	II					2099:2100	II	2099:2100	II	2099:2100	Compared to intact discs, expression of both collagen types I and II increased significantly in nHDF-treated discs (p<.05), trending to significant in RDF-treated discs, and not significantly in saline treated discs.
30142460	15	93	theme	collagen	2078:2085	arg1	I					2093:2093	I	2093:2093	I	2093:2093	Compared to intact discs, expression of both collagen types I and II increased significantly in nHDF-treated discs (p<.05), trending to significant in RDF-treated discs, and not significantly in saline treated discs.
30142460	4	94	theme	sources	663:669	arg1	disadvantages					631:643	disadvantages	631:643	disadvantages	631:643	Studies to determine the advantages and disadvantages of cell types and sources for therapy are needed.
30142460	4	94	theme	sources	663:669	arg1	advantages					616:625	advantages	616:625	advantages	616:625	Studies to determine the advantages and disadvantages of cell types and sources for therapy are needed.
30142460	13	95	theme	disc	1731:1734	arg1	weeks					1708:1712	Eight weeks	1702:1712	Eight weeks after treatment	1702:1728	RESULTS Eight weeks after treatment, disc height indexes of discs treated with nHDF increased significantly by 7.8% (p<.01), whereas those treated with saline or RDF increased by 1.5% and 2.0%, respectively.
30142460	13	95	theme	disc	1731:1734	arg1	indexes					1743:1749	disc height indexes	1731:1749	disc height indexes of discs treated with nHDF	1731:1776	RESULTS Eight weeks after treatment, disc height indexes of discs treated with nHDF increased significantly by 7.8% (p<.01), whereas those treated with saline or RDF increased by 1.5% and 2.0%, respectively.
30142460	19	96	theme	regenerative	2879:2890	arg1	trends					2892:2897	similar regenerative trends	2871:2897	similar regenerative trends	2871:2897	Transplantation with RDFs showed similar regenerative trends, but these trends were not significant.
30142460	6	97	theme	neonatal	957:964	arg1	nHDFs					992:996	nHDFs	992:996	nHDFs	992:996	STUDY DESIGN Using the rabbit disc degeneration model, we compared transplantation of neonatal human dermal fibroblasts (nHDFs) and rabbit dermal fibroblasts (RDFs) into rabbit degenerated discs on host immune response, disc height, and IVD composition.
30142460	6	97	theme	neonatal	957:964	arg1	fibroblasts					979:989	neonatal human dermal fibroblasts	957:989	neonatal human dermal fibroblasts (nHDFs)	957:997	STUDY DESIGN Using the rabbit disc degeneration model, we compared transplantation of neonatal human dermal fibroblasts (nHDFs) and rabbit dermal fibroblasts (RDFs) into rabbit degenerated discs on host immune response, disc height, and IVD composition.
30142460	10	98	theme	X-ray	1385:1389	arg1	images					1391:1396	X-ray images	1385:1396	X-ray images	1385:1396	X-ray images were obtained.
30142460	14	99	dep	displayed	1978:1986	arg1	.8					2028:2029	.8	2028:2029	.8	2028:2029	Gene expression analysis showed that discs transplanted with nHDFs and RDFs displayed similar inflammatory responses (p=.2 to .8).
30142460	14	100	dep	.8	2028:2029	arg1	to					2025:2026	to	2025:2026	to	2025:2026	Gene expression analysis showed that discs transplanted with nHDFs and RDFs displayed similar inflammatory responses (p=.2 to .8).
30142460	19	101	with	Transplantation	2838:2852	arg1	RDFs					2859:2862	RDFs	2859:2862	RDFs	2859:2862	Transplantation with RDFs showed similar regenerative trends, but these trends were not significant.
30142460	11	102	theme	gene	1462:1465	arg1	expression					1467:1476	collagen gene expression	1453:1476	collagen gene expression	1453:1476	IVDs were isolated for inflammatory and collagen gene expression analysis using real-time polymerase chain reaction and biochemical analysis of proteoglycan contents using dimethylmethylene blue assay.
30142460	7	103	theme	disc	1223:1226	arg1	degeneration					1228:1239	disc degeneration	1223:1239	disc degeneration	1223:1239	METHODS New Zealand white rabbits received an annular puncture using an 18-guage needle to induce disc degeneration.
30142460	1	104	theme	BACKGROUND	144:153	arg1	evidence					174:181	BACKGROUND CONTEXT Increasing evidence	144:181	BACKGROUND CONTEXT Increasing evidence	144:181	BACKGROUND CONTEXT Increasing evidence suggests transplanting viable cells into the degenerating intervertebral disc (IVD) may be effective in treating disc degeneration and back pain.
30142460	11	105	theme	chain	1514:1518	arg1	reaction					1520:1527	real-time polymerase chain reaction	1493:1527	real-time polymerase chain reaction	1493:1527	IVDs were isolated for inflammatory and collagen gene expression analysis using real-time polymerase chain reaction and biochemical analysis of proteoglycan contents using dimethylmethylene blue assay.
30142460	0	106	theme	rabbit	79:84	arg1	fibroblasts					93:103	rabbit dermal fibroblasts	79:103	rabbit dermal fibroblasts	79:103	Therapeutic effects of cell therapy with neonatal human dermal fibroblasts and rabbit dermal fibroblasts on disc degeneration and inflammation.
30142460	1	107	theme	Increasing	163:172	arg1	evidence					174:181	BACKGROUND CONTEXT Increasing evidence	144:181	BACKGROUND CONTEXT Increasing evidence	144:181	BACKGROUND CONTEXT Increasing evidence suggests transplanting viable cells into the degenerating intervertebral disc (IVD) may be effective in treating disc degeneration and back pain.
30142460	18	108	theme	disc	2757:2760	arg1	height					2762:2767	disc height	2757:2767	disc height	2757:2767	CONCLUSIONS This study showed that cell transplantation with nHDF into degenerated IVDs can significantly increase markers of disc regeneration (disc height, collagen type I and II gene expression, and proteoglycan contents).
30142460	6	109	theme	fibroblasts	1017:1027	arg1	transplantation					938:952	transplantation	938:952	transplantation of neonatal human dermal fibroblasts (nHDFs) and rabbit dermal fibroblasts (RDFs) into rabbit degenerated discs on host immune response, disc height, and IVD composition	938:1122	STUDY DESIGN Using the rabbit disc degeneration model, we compared transplantation of neonatal human dermal fibroblasts (nHDFs) and rabbit dermal fibroblasts (RDFs) into rabbit degenerated discs on host immune response, disc height, and IVD composition.
30142460	0	110	from	effects	12:18	arg1	inflammation					130:141	inflammation	130:141	inflammation	130:141	Therapeutic effects of cell therapy with neonatal human dermal fibroblasts and rabbit dermal fibroblasts on disc degeneration and inflammation.
30142460	0	110	from	effects	12:18	arg1	degeneration					113:124	disc degeneration	108:124	disc degeneration	108:124	Therapeutic effects of cell therapy with neonatal human dermal fibroblasts and rabbit dermal fibroblasts on disc degeneration and inflammation.
30142460	11	111	theme	real-time	1493:1501	arg1	reaction					1520:1527	real-time polymerase chain reaction	1493:1527	real-time polymerase chain reaction	1493:1527	IVDs were isolated for inflammatory and collagen gene expression analysis using real-time polymerase chain reaction and biochemical analysis of proteoglycan contents using dimethylmethylene blue assay.
30142460	4	112	dep	advantages	616:625	arg1	the					612:614	the	612:614	the	612:614	Studies to determine the advantages and disadvantages of cell types and sources for therapy are needed.
30142460	7	113	theme	18-guage	1197:1204	arg1	needle					1206:1211	an 18-guage needle	1194:1211	an 18-guage needle to induce disc degeneration	1194:1239	METHODS New Zealand white rabbits received an annular puncture using an 18-guage needle to induce disc degeneration.
30142460	5	114	from	effects	793:799	arg1	inflammation					857:868	inflammation	857:868	inflammation	857:868	PURPOSE The objective of this study is to determine the impact of donor source on the therapeutic effects of dermal fibroblast treatment on disc degeneration and inflammation.
30142460	5	114	from	effects	793:799	arg1	degeneration					840:851	disc degeneration	835:851	disc degeneration	835:851	PURPOSE The objective of this study is to determine the impact of donor source on the therapeutic effects of dermal fibroblast treatment on disc degeneration and inflammation.
30142460	6	115	theme	rabbit	1003:1008	arg1	RDFs					1030:1033	RDFs	1030:1033	RDFs	1030:1033	STUDY DESIGN Using the rabbit disc degeneration model, we compared transplantation of neonatal human dermal fibroblasts (nHDFs) and rabbit dermal fibroblasts (RDFs) into rabbit degenerated discs on host immune response, disc height, and IVD composition.
30142460	6	115	theme	rabbit	1003:1008	arg1	fibroblasts					1017:1027	rabbit dermal fibroblasts	1003:1027	rabbit dermal fibroblasts (RDFs)	1003:1034	STUDY DESIGN Using the rabbit disc degeneration model, we compared transplantation of neonatal human dermal fibroblasts (nHDFs) and rabbit dermal fibroblasts (RDFs) into rabbit degenerated discs on host immune response, disc height, and IVD composition.
30142460	0	116	theme	disc	108:111	arg1	degeneration					113:124	disc degeneration	108:124	disc degeneration	108:124	Therapeutic effects of cell therapy with neonatal human dermal fibroblasts and rabbit dermal fibroblasts on disc degeneration and inflammation.
30142460	5	117	theme	fibroblast	811:820	arg1	treatment					822:830	dermal fibroblast treatment	804:830	dermal fibroblast treatment	804:830	PURPOSE The objective of this study is to determine the impact of donor source on the therapeutic effects of dermal fibroblast treatment on disc degeneration and inflammation.
30142460	18	118	theme	disc	2738:2741	arg1	regeneration					2743:2754	disc regeneration	2738:2754	disc regeneration	2738:2754	CONCLUSIONS This study showed that cell transplantation with nHDF into degenerated IVDs can significantly increase markers of disc regeneration (disc height, collagen type I and II gene expression, and proteoglycan contents).
30142460	6	119	theme	immune	1074:1079	arg1	response					1081:1088	host immune response	1069:1088	host immune response	1069:1088	STUDY DESIGN Using the rabbit disc degeneration model, we compared transplantation of neonatal human dermal fibroblasts (nHDFs) and rabbit dermal fibroblasts (RDFs) into rabbit degenerated discs on host immune response, disc height, and IVD composition.
30142460	2	120	dep	studies	338:344	arg1	treat					407:411	treat	407:411	to treat patients with back pain	404:435	Clinical studies utilizing autologous or allogeneic mesenchymal stem cells to treat patients with back pain have reported some encouraging results.
30142460	0	121	theme	cell	23:26	arg1	therapy					28:34	cell therapy	23:34	cell therapy with neonatal human dermal fibroblasts and rabbit dermal fibroblasts	23:103	Therapeutic effects of cell therapy with neonatal human dermal fibroblasts and rabbit dermal fibroblasts on disc degeneration and inflammation.
30142460	1	122	theme	disc	296:299	arg1	degeneration					301:312	disc degeneration	296:312	disc degeneration	296:312	BACKGROUND CONTEXT Increasing evidence suggests transplanting viable cells into the degenerating intervertebral disc (IVD) may be effective in treating disc degeneration and back pain.
30142460	19	123	theme	similar	2871:2877	arg1	trends					2892:2897	similar regenerative trends	2871:2897	similar regenerative trends	2871:2897	Transplantation with RDFs showed similar regenerative trends, but these trends were not significant.
30142460	7	124	theme	white	1145:1149	arg1	rabbits					1151:1157	New Zealand white rabbits	1133:1157	New Zealand white rabbits	1133:1157	METHODS New Zealand white rabbits received an annular puncture using an 18-guage needle to induce disc degeneration.
30142460	6	125	theme	degenerated	1048:1058	arg1	discs					1060:1064	rabbit degenerated discs	1041:1064	rabbit degenerated discs on host immune response, disc height, and IVD composition	1041:1122	STUDY DESIGN Using the rabbit disc degeneration model, we compared transplantation of neonatal human dermal fibroblasts (nHDFs) and rabbit dermal fibroblasts (RDFs) into rabbit degenerated discs on host immune response, disc height, and IVD composition.
30142460	18	126	dep	increase	2718:2725	arg1	height					2762:2767	disc height	2757:2767	disc height	2757:2767	CONCLUSIONS This study showed that cell transplantation with nHDF into degenerated IVDs can significantly increase markers of disc regeneration (disc height, collagen type I and II gene expression, and proteoglycan contents).
30142460	18	126	dep	increase	2718:2725	arg1	type					2779:2782	collagen type I	2770:2784	collagen type I	2770:2784	CONCLUSIONS This study showed that cell transplantation with nHDF into degenerated IVDs can significantly increase markers of disc regeneration (disc height, collagen type I and II gene expression, and proteoglycan contents).
30142460	18	126	dep	increase	2718:2725	arg1	contents					2827:2834	proteoglycan contents	2814:2834	proteoglycan contents	2814:2834	CONCLUSIONS This study showed that cell transplantation with nHDF into degenerated IVDs can significantly increase markers of disc regeneration (disc height, collagen type I and II gene expression, and proteoglycan contents).
30142460	7	127	theme	New	1133:1135	arg1	rabbits					1151:1157	New Zealand white rabbits	1133:1157	New Zealand white rabbits	1133:1157	METHODS New Zealand white rabbits received an annular puncture using an 18-guage needle to induce disc degeneration.
30142460	15	128	theme	RDF-treated	2184:2194	arg1	discs					2196:2200	RDF-treated discs	2184:2200	RDF-treated discs	2184:2200	Compared to intact discs, expression of both collagen types I and II increased significantly in nHDF-treated discs (p<.05), trending to significant in RDF-treated discs, and not significantly in saline treated discs.
30142460	13	129	dep	increased	1778:1786	arg1	whereas					1819:1825	whereas	1819:1825	whereas	1819:1825	RESULTS Eight weeks after treatment, disc height indexes of discs treated with nHDF increased significantly by 7.8% (p<.01), whereas those treated with saline or RDF increased by 1.5% and 2.0%, respectively.
30142460	6	130	theme	STUDY	871:875	arg1	DESIGN					877:882	STUDY DESIGN	871:882	STUDY DESIGN	871:882	STUDY DESIGN Using the rabbit disc degeneration model, we compared transplantation of neonatal human dermal fibroblasts (nHDFs) and rabbit dermal fibroblasts (RDFs) into rabbit degenerated discs on host immune response, disc height, and IVD composition.
30142460	14	131	theme	expression	1907:1916	arg1	analysis					1918:1925	Gene expression analysis	1902:1925	Gene expression analysis	1902:1925	Gene expression analysis showed that discs transplanted with nHDFs and RDFs displayed similar inflammatory responses (p=.2 to .8).
30142460	3	132	contain	have	564:567	arg2	effects					582:588	regenerative effects	569:588	regenerative effects	569:588	Animal studies have shown that cells injected into the disc can survive for months and have regenerative effects.
30142460	3	132	contain	have	564:567	arg1	cells					508:512	cells	508:512	cells injected into the disc	508:535	Animal studies have shown that cells injected into the disc can survive for months and have regenerative effects.
30142460	11	133	theme	blue	1603:1606	arg1	assay					1608:1612	dimethylmethylene blue assay	1585:1612	dimethylmethylene blue assay	1585:1612	IVDs were isolated for inflammatory and collagen gene expression analysis using real-time polymerase chain reaction and biochemical analysis of proteoglycan contents using dimethylmethylene blue assay.
30142460	2	134	theme	autologous	356:365	arg1	cells					398:402	autologous or allogeneic mesenchymal stem cells	356:402	autologous or allogeneic mesenchymal stem cells	356:402	Clinical studies utilizing autologous or allogeneic mesenchymal stem cells to treat patients with back pain have reported some encouraging results.
30142460	11	135	theme	contents	1570:1577	arg1	analysis					1545:1552	biochemical analysis	1533:1552	biochemical analysis of proteoglycan contents	1533:1577	IVDs were isolated for inflammatory and collagen gene expression analysis using real-time polymerase chain reaction and biochemical analysis of proteoglycan contents using dimethylmethylene blue assay.
30142460	11	135	theme	contents	1570:1577	arg1	reaction					1520:1527	real-time polymerase chain reaction	1493:1527	real-time polymerase chain reaction	1493:1527	IVDs were isolated for inflammatory and collagen gene expression analysis using real-time polymerase chain reaction and biochemical analysis of proteoglycan contents using dimethylmethylene blue assay.
30142460	14	136	theme	similar	1988:1994	arg1	responses					2009:2017	similar inflammatory responses	1988:2017	similar inflammatory responses	1988:2017	Gene expression analysis showed that discs transplanted with nHDFs and RDFs displayed similar inflammatory responses (p=.2 to .8).
30142460	2	137	theme	stem	393:396	arg1	cells					398:402	autologous or allogeneic mesenchymal stem cells	356:402	autologous or allogeneic mesenchymal stem cells	356:402	Clinical studies utilizing autologous or allogeneic mesenchymal stem cells to treat patients with back pain have reported some encouraging results.
30142460	8	138	theme	rabbit	1267:1272	arg1	IVDs					1274:1277	rabbit IVDs	1267:1277	rabbit IVDs	1267:1277	Four weeks after injury, rabbit IVDs were treated with 5 × 106 nHDFs, RDFs, or saline.
30142460	4	139	theme	cell	648:651	arg1	types					653:657	cell types	648:657	cell types	648:657	Studies to determine the advantages and disadvantages of cell types and sources for therapy are needed.
30142460	18	140	theme	degenerated	2683:2693	arg1	IVDs					2695:2698	degenerated IVDs	2683:2698	degenerated IVDs	2683:2698	CONCLUSIONS This study showed that cell transplantation with nHDF into degenerated IVDs can significantly increase markers of disc regeneration (disc height, collagen type I and II gene expression, and proteoglycan contents).
30142460	7	141	theme	annular	1171:1177	arg1	puncture					1179:1186	an annular puncture	1168:1186	an annular puncture using an 18-guage needle to induce disc degeneration	1168:1239	METHODS New Zealand white rabbits received an annular puncture using an 18-guage needle to induce disc degeneration.
31025041	0	0	theme	resistant	58:66	arg1	starch					68:73	resistant starch	58:73	resistant starch	58:73	The beneficial effects of purple yam (Dioscorea alata L.) resistant starch on hyperlipidemia in high-fat-fed hamsters.
31025041	1	1	from	metabolism	267:276	arg1	hamsters					315:322	hyperlipidemic hamsters	300:322	hyperlipidemic hamsters	300:322	In this study, we investigated the interventional effect of resistant starch (RS) obtained from purple yam (Dioscorea alata L.) on regulating lipid metabolism and gut microbiota in hyperlipidemic hamsters.
31025041	3	2	theme	colon	845:849	arg1	histomorphology					812:826	the histomorphology	808:826	the histomorphology of the cecum and colon	808:849	Fat accumulation in the liver was reduced by RS, and the injury caused due to a high-fat diet on the histomorphology of the cecum and colon was repaired.
31025041	1	3	theme	purple	215:220	arg1	yam					222:224	purple yam	215:224	purple yam (Dioscorea alata L.)	215:245	In this study, we investigated the interventional effect of resistant starch (RS) obtained from purple yam (Dioscorea alata L.) on regulating lipid metabolism and gut microbiota in hyperlipidemic hamsters.
31025041	1	3	theme	purple	215:220	arg1	L.					243:244	L.	243:244	L.	243:244	In this study, we investigated the interventional effect of resistant starch (RS) obtained from purple yam (Dioscorea alata L.) on regulating lipid metabolism and gut microbiota in hyperlipidemic hamsters.
31025041	2	4	theme	increased	507:515	arg1	concentration					562:574	increased high-density lipoprotein cholesterol (HDL-C) concentration	507:574	increased high-density lipoprotein cholesterol (HDL-C) concentration	507:574	Compared to low level RS (LR) treatment, high dosage of RS (HR) administration in hamsters was shown to be more efficient in controlling the body weight and adipose tissue mass, and increased high-density lipoprotein cholesterol (HDL-C) concentration and decreased triglyceride (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) concentrations were observed.
31025041	6	5	theme	purple	1253:1258	arg1	yam					1260:1262	purple yam	1253:1262	purple yam	1253:1262	These results suggested that the usage of RS obtained from purple yam could ameliorate lipid metabolism in association with gut microbiota modulation, which could provide references for further medication.
31025041	1	6	theme	hyperlipidemic	300:313	arg1	hamsters					315:322	hyperlipidemic hamsters	300:322	hyperlipidemic hamsters	300:322	In this study, we investigated the interventional effect of resistant starch (RS) obtained from purple yam (Dioscorea alata L.) on regulating lipid metabolism and gut microbiota in hyperlipidemic hamsters.
31025041	2	7	theme	administration	389:402	arg1	dosage					371:376	high dosage	366:376	high dosage of RS (HR) administration in hamsters	366:414	Compared to low level RS (LR) treatment, high dosage of RS (HR) administration in hamsters was shown to be more efficient in controlling the body weight and adipose tissue mass, and increased high-density lipoprotein cholesterol (HDL-C) concentration and decreased triglyceride (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) concentrations were observed.
31025041	6	8	theme	gut	1318:1320	arg1	modulation					1333:1342	gut microbiota modulation	1318:1342	gut microbiota modulation	1318:1342	These results suggested that the usage of RS obtained from purple yam could ameliorate lipid metabolism in association with gut microbiota modulation, which could provide references for further medication.
31025041	3	9	theme	cecum	835:839	arg1	histomorphology					812:826	the histomorphology	808:826	the histomorphology of the cecum and colon	808:849	Fat accumulation in the liver was reduced by RS, and the injury caused due to a high-fat diet on the histomorphology of the cecum and colon was repaired.
31025041	2	10	theme	cholesterol	661:671	arg1	concentrations					681:694	decreased triglyceride (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) concentrations	580:694	decreased triglyceride (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) concentrations	580:694	Compared to low level RS (LR) treatment, high dosage of RS (HR) administration in hamsters was shown to be more efficient in controlling the body weight and adipose tissue mass, and increased high-density lipoprotein cholesterol (HDL-C) concentration and decreased triglyceride (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) concentrations were observed.
31025041	6	11	with	association	1301:1311	arg1	modulation					1333:1342	gut microbiota modulation	1318:1342	gut microbiota modulation	1318:1342	These results suggested that the usage of RS obtained from purple yam could ameliorate lipid metabolism in association with gut microbiota modulation, which could provide references for further medication.
31025041	2	12	theme	lipoprotein	649:659	arg1	LDL-C					674:678	LDL-C	674:678	LDL-C	674:678	Compared to low level RS (LR) treatment, high dosage of RS (HR) administration in hamsters was shown to be more efficient in controlling the body weight and adipose tissue mass, and increased high-density lipoprotein cholesterol (HDL-C) concentration and decreased triglyceride (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) concentrations were observed.
31025041	2	12	theme	lipoprotein	649:659	arg1	cholesterol					661:671	low-density lipoprotein cholesterol	637:671	low-density lipoprotein cholesterol (LDL-C)	637:679	Compared to low level RS (LR) treatment, high dosage of RS (HR) administration in hamsters was shown to be more efficient in controlling the body weight and adipose tissue mass, and increased high-density lipoprotein cholesterol (HDL-C) concentration and decreased triglyceride (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) concentrations were observed.
31025041	6	13	theme	RS	1236:1237	arg1	usage					1227:1231	the usage	1223:1231	the usage of RS obtained from purple yam	1223:1262	These results suggested that the usage of RS obtained from purple yam could ameliorate lipid metabolism in association with gut microbiota modulation, which could provide references for further medication.
31025041	2	14	from	administration	389:402	arg1	hamsters					407:414	hamsters	407:414	hamsters	407:414	Compared to low level RS (LR) treatment, high dosage of RS (HR) administration in hamsters was shown to be more efficient in controlling the body weight and adipose tissue mass, and increased high-density lipoprotein cholesterol (HDL-C) concentration and decreased triglyceride (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) concentrations were observed.
31025041	2	15	theme	cholesterol	542:552	arg1	concentration					562:574	increased high-density lipoprotein cholesterol (HDL-C) concentration	507:574	increased high-density lipoprotein cholesterol (HDL-C) concentration	507:574	Compared to low level RS (LR) treatment, high dosage of RS (HR) administration in hamsters was shown to be more efficient in controlling the body weight and adipose tissue mass, and increased high-density lipoprotein cholesterol (HDL-C) concentration and decreased triglyceride (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) concentrations were observed.
31025041	0	16	dep	starch	68:73	arg1	L.					54:55	L.	54:55	L.	54:55	The beneficial effects of purple yam (Dioscorea alata L.) resistant starch on hyperlipidemia in high-fat-fed hamsters.
31025041	2	17	theme	low-density	637:647	arg1	LDL-C					674:678	LDL-C	674:678	LDL-C	674:678	Compared to low level RS (LR) treatment, high dosage of RS (HR) administration in hamsters was shown to be more efficient in controlling the body weight and adipose tissue mass, and increased high-density lipoprotein cholesterol (HDL-C) concentration and decreased triglyceride (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) concentrations were observed.
31025041	2	17	theme	low-density	637:647	arg1	cholesterol					661:671	low-density lipoprotein cholesterol	637:671	low-density lipoprotein cholesterol (LDL-C)	637:679	Compared to low level RS (LR) treatment, high dosage of RS (HR) administration in hamsters was shown to be more efficient in controlling the body weight and adipose tissue mass, and increased high-density lipoprotein cholesterol (HDL-C) concentration and decreased triglyceride (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) concentrations were observed.
31025041	2	18	theme	RS	347:348	arg1	treatment					355:363	low level RS (LR) treatment	337:363	low level RS (LR) treatment	337:363	Compared to low level RS (LR) treatment, high dosage of RS (HR) administration in hamsters was shown to be more efficient in controlling the body weight and adipose tissue mass, and increased high-density lipoprotein cholesterol (HDL-C) concentration and decreased triglyceride (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) concentrations were observed.
31025041	0	19	theme	beneficial	4:13	arg1	effects					15:21	The beneficial effects	0:21	The beneficial effects of purple yam	0:35	The beneficial effects of purple yam (Dioscorea alata L.) resistant starch on hyperlipidemia in high-fat-fed hamsters.
31025041	2	20	theme	lipoprotein	530:540	arg1	HDL-C					555:559	HDL-C	555:559	HDL-C	555:559	Compared to low level RS (LR) treatment, high dosage of RS (HR) administration in hamsters was shown to be more efficient in controlling the body weight and adipose tissue mass, and increased high-density lipoprotein cholesterol (HDL-C) concentration and decreased triglyceride (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) concentrations were observed.
31025041	2	20	theme	lipoprotein	530:540	arg1	cholesterol					542:552	high-density lipoprotein cholesterol	517:552	increased high-density lipoprotein cholesterol (HDL-C) concentration	507:574	Compared to low level RS (LR) treatment, high dosage of RS (HR) administration in hamsters was shown to be more efficient in controlling the body weight and adipose tissue mass, and increased high-density lipoprotein cholesterol (HDL-C) concentration and decreased triglyceride (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) concentrations were observed.
31025041	3	21	dep	cecum	835:839	arg1	the					831:833	the	831:833	the	831:833	Fat accumulation in the liver was reduced by RS, and the injury caused due to a high-fat diet on the histomorphology of the cecum and colon was repaired.
31025041	1	22	theme	interventional	154:167	arg1	effect					169:174	the interventional effect	150:174	the interventional effect of resistant starch (RS) obtained from purple yam (Dioscorea alata L.) on regulating lipid metabolism and gut microbiota in hyperlipidemic hamsters	150:322	In this study, we investigated the interventional effect of resistant starch (RS) obtained from purple yam (Dioscorea alata L.) on regulating lipid metabolism and gut microbiota in hyperlipidemic hamsters.
31025041	2	23	theme	high-density	517:528	arg1	HDL-C					555:559	HDL-C	555:559	HDL-C	555:559	Compared to low level RS (LR) treatment, high dosage of RS (HR) administration in hamsters was shown to be more efficient in controlling the body weight and adipose tissue mass, and increased high-density lipoprotein cholesterol (HDL-C) concentration and decreased triglyceride (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) concentrations were observed.
31025041	2	23	theme	high-density	517:528	arg1	cholesterol					542:552	high-density lipoprotein cholesterol	517:552	increased high-density lipoprotein cholesterol (HDL-C) concentration	507:574	Compared to low level RS (LR) treatment, high dosage of RS (HR) administration in hamsters was shown to be more efficient in controlling the body weight and adipose tissue mass, and increased high-density lipoprotein cholesterol (HDL-C) concentration and decreased triglyceride (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) concentrations were observed.
31025041	2	24	theme	triglyceride	590:601	arg1	concentrations					681:694	decreased triglyceride (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) concentrations	580:694	decreased triglyceride (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) concentrations	580:694	Compared to low level RS (LR) treatment, high dosage of RS (HR) administration in hamsters was shown to be more efficient in controlling the body weight and adipose tissue mass, and increased high-density lipoprotein cholesterol (HDL-C) concentration and decreased triglyceride (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) concentrations were observed.
31025041	2	25	theme	low	337:339	arg1	RS					347:348	low level RS	337:348	low level RS (LR) treatment	337:363	Compared to low level RS (LR) treatment, high dosage of RS (HR) administration in hamsters was shown to be more efficient in controlling the body weight and adipose tissue mass, and increased high-density lipoprotein cholesterol (HDL-C) concentration and decreased triglyceride (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) concentrations were observed.
31025041	2	25	theme	low	337:339	arg1	LR					351:352	LR	351:352	LR	351:352	Compared to low level RS (LR) treatment, high dosage of RS (HR) administration in hamsters was shown to be more efficient in controlling the body weight and adipose tissue mass, and increased high-density lipoprotein cholesterol (HDL-C) concentration and decreased triglyceride (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) concentrations were observed.
31025041	4	26	theme	community	897:905	arg1	composition					907:917	the gut community composition	889:917	the gut community composition	889:917	Furthermore, HR altered the gut community composition by increasing the relative abundances of Bifidobacteria, Lactobacillus, Coprococcus, and Allobaculum, while decreasing Parabacteroides and Dorea.
31025041	2	27	theme	body	466:469	arg1	weight					471:476	the body weight	462:476	the body weight	462:476	Compared to low level RS (LR) treatment, high dosage of RS (HR) administration in hamsters was shown to be more efficient in controlling the body weight and adipose tissue mass, and increased high-density lipoprotein cholesterol (HDL-C) concentration and decreased triglyceride (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) concentrations were observed.
31025041	2	28	theme	level	341:345	arg1	RS					347:348	low level RS	337:348	low level RS (LR) treatment	337:363	Compared to low level RS (LR) treatment, high dosage of RS (HR) administration in hamsters was shown to be more efficient in controlling the body weight and adipose tissue mass, and increased high-density lipoprotein cholesterol (HDL-C) concentration and decreased triglyceride (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) concentrations were observed.
31025041	2	28	theme	level	341:345	arg1	LR					351:352	LR	351:352	LR	351:352	Compared to low level RS (LR) treatment, high dosage of RS (HR) administration in hamsters was shown to be more efficient in controlling the body weight and adipose tissue mass, and increased high-density lipoprotein cholesterol (HDL-C) concentration and decreased triglyceride (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) concentrations were observed.
31025041	2	29	from	dosage	371:376	arg1	hamsters					407:414	hamsters	407:414	hamsters	407:414	Compared to low level RS (LR) treatment, high dosage of RS (HR) administration in hamsters was shown to be more efficient in controlling the body weight and adipose tissue mass, and increased high-density lipoprotein cholesterol (HDL-C) concentration and decreased triglyceride (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) concentrations were observed.
31025041	2	30	theme	high	366:369	arg1	dosage					371:376	high dosage	366:376	high dosage of RS (HR) administration in hamsters	366:414	Compared to low level RS (LR) treatment, high dosage of RS (HR) administration in hamsters was shown to be more efficient in controlling the body weight and adipose tissue mass, and increased high-density lipoprotein cholesterol (HDL-C) concentration and decreased triglyceride (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) concentrations were observed.
31025041	0	31	theme	high-fat-fed	96:107	arg1	hamsters					109:116	high-fat-fed hamsters	96:116	high-fat-fed hamsters	96:116	The beneficial effects of purple yam (Dioscorea alata L.) resistant starch on hyperlipidemia in high-fat-fed hamsters.
31025041	2	32	from	hamsters	407:414	arg1	dosage					371:376	high dosage	366:376	high dosage of RS (HR) administration in hamsters	366:414	Compared to low level RS (LR) treatment, high dosage of RS (HR) administration in hamsters was shown to be more efficient in controlling the body weight and adipose tissue mass, and increased high-density lipoprotein cholesterol (HDL-C) concentration and decreased triglyceride (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) concentrations were observed.
31025041	3	33	theme	high-fat	791:798	arg1	diet					800:803	a high-fat diet	789:803	a high-fat diet on the histomorphology of the cecum and colon	789:849	Fat accumulation in the liver was reduced by RS, and the injury caused due to a high-fat diet on the histomorphology of the cecum and colon was repaired.
31025041	1	34	theme	resistant	179:187	arg1	RS					197:198	RS	197:198	RS	197:198	In this study, we investigated the interventional effect of resistant starch (RS) obtained from purple yam (Dioscorea alata L.) on regulating lipid metabolism and gut microbiota in hyperlipidemic hamsters.
31025041	1	34	theme	resistant	179:187	arg1	starch					189:194	resistant starch	179:194	resistant starch (RS) obtained from purple yam (Dioscorea alata L.)	179:245	In this study, we investigated the interventional effect of resistant starch (RS) obtained from purple yam (Dioscorea alata L.) on regulating lipid metabolism and gut microbiota in hyperlipidemic hamsters.
31025041	0	35	theme	yam	33:35	arg1	effects					15:21	The beneficial effects	0:21	The beneficial effects of purple yam	0:35	The beneficial effects of purple yam (Dioscorea alata L.) resistant starch on hyperlipidemia in high-fat-fed hamsters.
31025041	1	36	theme	starch	189:194	arg1	effect					169:174	the interventional effect	150:174	the interventional effect of resistant starch (RS) obtained from purple yam (Dioscorea alata L.) on regulating lipid metabolism and gut microbiota in hyperlipidemic hamsters	150:322	In this study, we investigated the interventional effect of resistant starch (RS) obtained from purple yam (Dioscorea alata L.) on regulating lipid metabolism and gut microbiota in hyperlipidemic hamsters.
31025041	0	37	theme	purple	26:31	arg1	yam					33:35	purple yam	26:35	purple yam	26:35	The beneficial effects of purple yam (Dioscorea alata L.) resistant starch on hyperlipidemia in high-fat-fed hamsters.
31025041	3	38	from	diet	800:803	arg1	histomorphology					812:826	the histomorphology	808:826	the histomorphology of the cecum and colon	808:849	Fat accumulation in the liver was reduced by RS, and the injury caused due to a high-fat diet on the histomorphology of the cecum and colon was repaired.
31025041	2	39	theme	cholesterol	615:625	arg1	concentrations					681:694	decreased triglyceride (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) concentrations	580:694	decreased triglyceride (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) concentrations	580:694	Compared to low level RS (LR) treatment, high dosage of RS (HR) administration in hamsters was shown to be more efficient in controlling the body weight and adipose tissue mass, and increased high-density lipoprotein cholesterol (HDL-C) concentration and decreased triglyceride (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) concentrations were observed.
31025041	1	40	theme	lipid	261:265	arg1	metabolism					267:276	lipid metabolism	261:276	lipid metabolism	261:276	In this study, we investigated the interventional effect of resistant starch (RS) obtained from purple yam (Dioscorea alata L.) on regulating lipid metabolism and gut microbiota in hyperlipidemic hamsters.
31025041	2	41	theme	tissue	490:495	arg1	mass					497:500	adipose tissue mass	482:500	adipose tissue mass	482:500	Compared to low level RS (LR) treatment, high dosage of RS (HR) administration in hamsters was shown to be more efficient in controlling the body weight and adipose tissue mass, and increased high-density lipoprotein cholesterol (HDL-C) concentration and decreased triglyceride (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) concentrations were observed.
31025041	2	42	theme	total	609:613	arg1	TC					628:629	TC	628:629	TC	628:629	Compared to low level RS (LR) treatment, high dosage of RS (HR) administration in hamsters was shown to be more efficient in controlling the body weight and adipose tissue mass, and increased high-density lipoprotein cholesterol (HDL-C) concentration and decreased triglyceride (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) concentrations were observed.
31025041	2	42	theme	total	609:613	arg1	cholesterol					615:625	total cholesterol	609:625	total cholesterol (TC)	609:630	Compared to low level RS (LR) treatment, high dosage of RS (HR) administration in hamsters was shown to be more efficient in controlling the body weight and adipose tissue mass, and increased high-density lipoprotein cholesterol (HDL-C) concentration and decreased triglyceride (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) concentrations were observed.
31025041	3	43	from	accumulation	715:726	arg1	liver					735:739	the liver	731:739	the liver	731:739	Fat accumulation in the liver was reduced by RS, and the injury caused due to a high-fat diet on the histomorphology of the cecum and colon was repaired.
31025041	1	44	from	microbiota	286:295	arg1	hamsters					315:322	hyperlipidemic hamsters	300:322	hyperlipidemic hamsters	300:322	In this study, we investigated the interventional effect of resistant starch (RS) obtained from purple yam (Dioscorea alata L.) on regulating lipid metabolism and gut microbiota in hyperlipidemic hamsters.
31025041	2	45	theme	adipose	482:488	arg1	mass					497:500	adipose tissue mass	482:500	adipose tissue mass	482:500	Compared to low level RS (LR) treatment, high dosage of RS (HR) administration in hamsters was shown to be more efficient in controlling the body weight and adipose tissue mass, and increased high-density lipoprotein cholesterol (HDL-C) concentration and decreased triglyceride (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) concentrations were observed.
31025041	2	46	theme	decreased	580:588	arg1	triglyceride					590:601	decreased triglyceride	580:601	decreased triglyceride (TG)	580:606	Compared to low level RS (LR) treatment, high dosage of RS (HR) administration in hamsters was shown to be more efficient in controlling the body weight and adipose tissue mass, and increased high-density lipoprotein cholesterol (HDL-C) concentration and decreased triglyceride (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) concentrations were observed.
31025041	2	46	theme	decreased	580:588	arg1	TG					604:605	TG	604:605	TG	604:605	Compared to low level RS (LR) treatment, high dosage of RS (HR) administration in hamsters was shown to be more efficient in controlling the body weight and adipose tissue mass, and increased high-density lipoprotein cholesterol (HDL-C) concentration and decreased triglyceride (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) concentrations were observed.
31025041	0	47	from	hyperlipidemia	78:91	arg1	hamsters					109:116	high-fat-fed hamsters	96:116	high-fat-fed hamsters	96:116	The beneficial effects of purple yam (Dioscorea alata L.) resistant starch on hyperlipidemia in high-fat-fed hamsters.
31025041	4	48	theme	relative	937:944	arg1	abundances					946:955	the relative abundances	933:955	the relative abundances of Bifidobacteria, Lactobacillus, Coprococcus, and Allobaculum,	933:1019	Furthermore, HR altered the gut community composition by increasing the relative abundances of Bifidobacteria, Lactobacillus, Coprococcus, and Allobaculum, while decreasing Parabacteroides and Dorea.
31025041	6	49	theme	microbiota	1322:1331	arg1	modulation					1333:1342	gut microbiota modulation	1318:1342	gut microbiota modulation	1318:1342	These results suggested that the usage of RS obtained from purple yam could ameliorate lipid metabolism in association with gut microbiota modulation, which could provide references for further medication.
31025041	4	50	theme	Bifidobacteria	960:973	arg1	abundances					946:955	the relative abundances	933:955	the relative abundances of Bifidobacteria, Lactobacillus, Coprococcus, and Allobaculum,	933:1019	Furthermore, HR altered the gut community composition by increasing the relative abundances of Bifidobacteria, Lactobacillus, Coprococcus, and Allobaculum, while decreasing Parabacteroides and Dorea.
31025041	3	51	theme	Fat	711:713	arg1	accumulation					715:726	Fat accumulation	711:726	Fat accumulation in the liver	711:739	Fat accumulation in the liver was reduced by RS, and the injury caused due to a high-fat diet on the histomorphology of the cecum and colon was repaired.
31025041	6	52	theme	lipid	1281:1285	arg1	metabolism					1287:1296	lipid metabolism	1281:1296	lipid metabolism	1281:1296	These results suggested that the usage of RS obtained from purple yam could ameliorate lipid metabolism in association with gut microbiota modulation, which could provide references for further medication.
31025041	0	53	from	starch	68:73	arg1	hyperlipidemia					78:91	hyperlipidemia	78:91	hyperlipidemia in high-fat-fed hamsters	78:116	The beneficial effects of purple yam (Dioscorea alata L.) resistant starch on hyperlipidemia in high-fat-fed hamsters.
31025041	6	54	theme	further	1380:1386	arg1	medication					1388:1397	further medication	1380:1397	further medication	1380:1397	These results suggested that the usage of RS obtained from purple yam could ameliorate lipid metabolism in association with gut microbiota modulation, which could provide references for further medication.
31025041	5	55	theme	blood	1174:1178	arg1	levels					1186:1191	blood lipid levels	1174:1191	blood lipid levels	1174:1191	Probiotics, such as Bifidobacteria and Lactobacillus, were dramatically increased and highly correlated with blood lipid levels.
31025041	1	56	theme	gut	282:284	arg1	microbiota					286:295	gut microbiota	282:295	gut microbiota	282:295	In this study, we investigated the interventional effect of resistant starch (RS) obtained from purple yam (Dioscorea alata L.) on regulating lipid metabolism and gut microbiota in hyperlipidemic hamsters.
31025041	4	57	theme	gut	893:895	arg1	composition					907:917	the gut community composition	889:917	the gut community composition	889:917	Furthermore, HR altered the gut community composition by increasing the relative abundances of Bifidobacteria, Lactobacillus, Coprococcus, and Allobaculum, while decreasing Parabacteroides and Dorea.
31025041	2	58	theme	RS	381:382	arg1	administration					389:402	RS (HR) administration	381:402	RS (HR) administration in hamsters	381:414	Compared to low level RS (LR) treatment, high dosage of RS (HR) administration in hamsters was shown to be more efficient in controlling the body weight and adipose tissue mass, and increased high-density lipoprotein cholesterol (HDL-C) concentration and decreased triglyceride (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) concentrations were observed.
31025041	5	59	theme	lipid	1180:1184	arg1	levels					1186:1191	blood lipid levels	1174:1191	blood lipid levels	1174:1191	Probiotics, such as Bifidobacteria and Lactobacillus, were dramatically increased and highly correlated with blood lipid levels.
31165292	3	0	from	absence	368:374	arg1	plates					391:396	R2A plates	387:396	R2A plates	387:396	Its optimum growth temperature was determined to be 28 °C in the absence of NaCl on R2A plates.
31165292	9	1	theme	60.5 mol	1375:1382	arg1	content					1346:1352	The DNA G+C content	1334:1352	The DNA G+C content	1334:1352	The DNA G+C content was determined to be 60.5 mol%.
31165292	9	1	theme	60.5 mol	1375:1382	arg1	%					1383:1383	60.5 mol%	1375:1383	60.5 mol%	1375:1383	The DNA G+C content was determined to be 60.5 mol%.
31165292	4	2	theme	rRNA	419:422	arg1	analysis					438:445	16S rRNA gene sequence analysis	415:445	16S rRNA gene sequence analysis	415:445	On the basis of 16S rRNA gene sequence analysis, strain sh-6T belongs to the genus Hymenobacter and is closely related to Hymenobacter deserti ZLB-3T (95.05%), Hymenobacter paludis KBP-30T (94.96%), Hymenobacter coalescens WW84T (94.04%), Hymenobacter gummosus ANT-18T (93.38%), Hymenobacter ocellatus Myx2105T (93.70%), Hymenobacter jeollabukensis 1-3-3-8T (93.48%) and Hymenobacter koreensis GYR3077T (93.21%).
31165292	5	3	theme	%	976:976	arg1	values					952:957	digital DNA-DNA hybridization and Average Nucleotide Identity values	890:957	digital DNA-DNA hybridization and Average Nucleotide Identity values of 20.6% and 78.4%, respectively	890:990	Comparison of the genome of strain sh-6T and that of H. gummosus ANT-18T gave digital DNA-DNA hybridization and Average Nucleotide Identity values of 20.6% and 78.4%, respectively.
31165292	5	4	theme	Identity	943:950	arg1	values					952:957	digital DNA-DNA hybridization and Average Nucleotide Identity values	890:957	digital DNA-DNA hybridization and Average Nucleotide Identity values of 20.6% and 78.4%, respectively	890:990	Comparison of the genome of strain sh-6T and that of H. gummosus ANT-18T gave digital DNA-DNA hybridization and Average Nucleotide Identity values of 20.6% and 78.4%, respectively.
31165292	5	5	theme	hybridization	906:918	arg1	values					952:957	digital DNA-DNA hybridization and Average Nucleotide Identity values	890:957	digital DNA-DNA hybridization and Average Nucleotide Identity values of 20.6% and 78.4%, respectively	890:990	Comparison of the genome of strain sh-6T and that of H. gummosus ANT-18T gave digital DNA-DNA hybridization and Average Nucleotide Identity values of 20.6% and 78.4%, respectively.
31165292	10	6	dep	species	1448:1454	arg1	evidence					1406:1413	the evidence	1402:1413	the evidence presented in this study	1402:1437	On the basis of the evidence presented in this study, a novel species of the genus Hymenobacter, Hymenobacter oligotrophus sp.
31165292	5	7	theme	Average	924:930	arg1	Identity					943:950	Average Nucleotide Identity	924:950	Average Nucleotide Identity	924:950	Comparison of the genome of strain sh-6T and that of H. gummosus ANT-18T gave digital DNA-DNA hybridization and Average Nucleotide Identity values of 20.6% and 78.4%, respectively.
31165292	4	8	theme	strain	448:453	arg1	sh-6T					455:459	strain sh-6T	448:459	strain sh-6T	448:459	On the basis of 16S rRNA gene sequence analysis, strain sh-6T belongs to the genus Hymenobacter and is closely related to Hymenobacter deserti ZLB-3T (95.05%), Hymenobacter paludis KBP-30T (94.96%), Hymenobacter coalescens WW84T (94.04%), Hymenobacter gummosus ANT-18T (93.38%), Hymenobacter ocellatus Myx2105T (93.70%), Hymenobacter jeollabukensis 1-3-3-8T (93.48%) and Hymenobacter koreensis GYR3077T (93.21%).
31165292	8	9	theme	major	1257:1261	arg1	lipid					1269:1273	The major polar lipid	1253:1273	The major polar lipid of strain	1253:1283	The major polar lipid of strain sh-6T determined to be phosphatidylethanolamine.
31165292	11	10	theme	AB	1568:1569	arg1	62345T					1587:1592	= CCTCC AB 2016064T = KCTC 62345T	1560:1592	= CCTCC AB 2016064T = KCTC 62345T	1560:1592	nov., is proposed, with the type strain sh-6T (= CCTCC AB 2016064T = KCTC 62345T).
31165292	11	10	theme	AB	1568:1569	arg1	sh-6T					1553:1557	the type strain sh-6T	1537:1557	the type strain sh-6T (= CCTCC AB 2016064T = KCTC 62345T)	1537:1593	nov., is proposed, with the type strain sh-6T (= CCTCC AB 2016064T = KCTC 62345T).
31165292	2	11	theme	motile	133:138	arg1	sh-6T					197:201	sh-6T	197:201	sh-6T	197:201	A taxonomic study of a Gram-stain negative, rod-shaped, motile, asporogenous, catalase- and oxidase-positive bacterium, sh-6T, forming pink-red colonies, isolated from a contaminated R2A plate in the laboratory was performed.
31165292	2	11	theme	motile	133:138	arg1	bacterium					186:194	a Gram-stain negative, rod-shaped, motile, asporogenous, catalase- and oxidase-positive bacterium	98:194	a Gram-stain negative, rod-shaped, motile, asporogenous, catalase- and oxidase-positive bacterium	98:194	A taxonomic study of a Gram-stain negative, rod-shaped, motile, asporogenous, catalase- and oxidase-positive bacterium, sh-6T, forming pink-red colonies, isolated from a contaminated R2A plate in the laboratory was performed.
31165292	2	12	theme	R2A	260:262	arg1	plate					264:268	a contaminated R2A plate	245:268	a contaminated R2A plate in the laboratory	245:286	A taxonomic study of a Gram-stain negative, rod-shaped, motile, asporogenous, catalase- and oxidase-positive bacterium, sh-6T, forming pink-red colonies, isolated from a contaminated R2A plate in the laboratory was performed.
31165292	1	13	theme	contaminated	52:63	arg1	plate					70:74	a contaminated agar plate	50:74	a contaminated agar plate	50:74	nov., isolated from a contaminated agar plate.
31165292	11	14	theme	2016064T = KCTC	1571:1585	arg1	62345T					1587:1592	= CCTCC AB 2016064T = KCTC 62345T	1560:1592	= CCTCC AB 2016064T = KCTC 62345T	1560:1592	nov., is proposed, with the type strain sh-6T (= CCTCC AB 2016064T = KCTC 62345T).
31165292	11	14	theme	2016064T = KCTC	1571:1585	arg1	sh-6T					1553:1557	the type strain sh-6T	1537:1557	the type strain sh-6T (= CCTCC AB 2016064T = KCTC 62345T)	1537:1593	nov., is proposed, with the type strain sh-6T (= CCTCC AB 2016064T = KCTC 62345T).
31165292	3	15	theme	growth	315:320	arg1	temperature					322:332	Its optimum growth temperature	303:332	Its optimum growth temperature	303:332	Its optimum growth temperature was determined to be 28 °C in the absence of NaCl on R2A plates.
31165292	4	16	theme	16S	415:417	arg1	analysis					438:445	16S rRNA gene sequence analysis	415:445	16S rRNA gene sequence analysis	415:445	On the basis of 16S rRNA gene sequence analysis, strain sh-6T belongs to the genus Hymenobacter and is closely related to Hymenobacter deserti ZLB-3T (95.05%), Hymenobacter paludis KBP-30T (94.96%), Hymenobacter coalescens WW84T (94.04%), Hymenobacter gummosus ANT-18T (93.38%), Hymenobacter ocellatus Myx2105T (93.70%), Hymenobacter jeollabukensis 1-3-3-8T (93.48%) and Hymenobacter koreensis GYR3077T (93.21%).
31165292	1	17	theme	agar	65:68	arg1	plate					70:74	a contaminated agar plate	50:74	a contaminated agar plate	50:74	nov., isolated from a contaminated agar plate.
31165292	10	18	theme	Hymenobacter	1469:1480	arg1	species					1448:1454	a novel species	1440:1454	a novel species of the genus Hymenobacter, Hymenobacter oligotrophus sp	1440:1510	On the basis of the evidence presented in this study, a novel species of the genus Hymenobacter, Hymenobacter oligotrophus sp.
31165292	7	19	theme	iso-C17:1	1188:1196	arg1	B					1208:1208	iso-C17:1 I/anteiso B	1188:1208	iso-C17:1 I/anteiso B	1188:1208	The major cellular fatty acids identified as iso-C15:0, summed feature 4 (iso-C17:1 I/anteiso B), iso-C16:0, iso-C17:0 3-OH and iso-C17:0.
31165292	7	19	theme	iso-C17:1	1188:1196	arg1	feature					1177:1183	summed feature 4	1170:1185	summed feature 4 (iso-C17:1 I/anteiso B)	1170:1209	The major cellular fatty acids identified as iso-C15:0, summed feature 4 (iso-C17:1 I/anteiso B), iso-C16:0, iso-C17:0 3-OH and iso-C17:0.
31165292	2	20	theme	rod-shaped	121:130	arg1	sh-6T					197:201	sh-6T	197:201	sh-6T	197:201	A taxonomic study of a Gram-stain negative, rod-shaped, motile, asporogenous, catalase- and oxidase-positive bacterium, sh-6T, forming pink-red colonies, isolated from a contaminated R2A plate in the laboratory was performed.
31165292	2	20	theme	rod-shaped	121:130	arg1	bacterium					186:194	a Gram-stain negative, rod-shaped, motile, asporogenous, catalase- and oxidase-positive bacterium	98:194	a Gram-stain negative, rod-shaped, motile, asporogenous, catalase- and oxidase-positive bacterium	98:194	A taxonomic study of a Gram-stain negative, rod-shaped, motile, asporogenous, catalase- and oxidase-positive bacterium, sh-6T, forming pink-red colonies, isolated from a contaminated R2A plate in the laboratory was performed.
31165292	4	21	dep	Hymenobacter	720:731	arg1	jeollabukensis					733:746	Hymenobacter jeollabukensis 1-3-3-8T (93.48%)	720:764	Hymenobacter jeollabukensis 1-3-3-8T (93.48%)	720:764	On the basis of 16S rRNA gene sequence analysis, strain sh-6T belongs to the genus Hymenobacter and is closely related to Hymenobacter deserti ZLB-3T (95.05%), Hymenobacter paludis KBP-30T (94.96%), Hymenobacter coalescens WW84T (94.04%), Hymenobacter gummosus ANT-18T (93.38%), Hymenobacter ocellatus Myx2105T (93.70%), Hymenobacter jeollabukensis 1-3-3-8T (93.48%) and Hymenobacter koreensis GYR3077T (93.21%).
31165292	4	21	dep	Hymenobacter	720:731	arg1	1-3-3-8T					748:755	1-3-3-8T	748:755	1-3-3-8T	748:755	On the basis of 16S rRNA gene sequence analysis, strain sh-6T belongs to the genus Hymenobacter and is closely related to Hymenobacter deserti ZLB-3T (95.05%), Hymenobacter paludis KBP-30T (94.96%), Hymenobacter coalescens WW84T (94.04%), Hymenobacter gummosus ANT-18T (93.38%), Hymenobacter ocellatus Myx2105T (93.70%), Hymenobacter jeollabukensis 1-3-3-8T (93.48%) and Hymenobacter koreensis GYR3077T (93.21%).
31165292	4	21	dep	Hymenobacter	720:731	arg1	%					763:763	93.48%	758:763	93.48%	758:763	On the basis of 16S rRNA gene sequence analysis, strain sh-6T belongs to the genus Hymenobacter and is closely related to Hymenobacter deserti ZLB-3T (95.05%), Hymenobacter paludis KBP-30T (94.96%), Hymenobacter coalescens WW84T (94.04%), Hymenobacter gummosus ANT-18T (93.38%), Hymenobacter ocellatus Myx2105T (93.70%), Hymenobacter jeollabukensis 1-3-3-8T (93.48%) and Hymenobacter koreensis GYR3077T (93.21%).
31165292	6	22	theme	polyamine	1032:1040	arg1	sym-homospermidine					1080:1097	sym-homospermidine	1080:1097	sym-homospermidine	1080:1097	The respiratory isoprenoid quinone and polyamine component were identified as MK-7 and sym-homospermidine, respectively.
31165292	6	22	theme	polyamine	1032:1040	arg1	MK-7					1071:1074	MK-7	1071:1074	MK-7	1071:1074	The respiratory isoprenoid quinone and polyamine component were identified as MK-7 and sym-homospermidine, respectively.
31165292	6	22	theme	polyamine	1032:1040	arg1	quinone					1020:1026	The respiratory isoprenoid quinone	993:1026	The respiratory isoprenoid quinone	993:1026	The respiratory isoprenoid quinone and polyamine component were identified as MK-7 and sym-homospermidine, respectively.
31165292	6	22	theme	polyamine	1032:1040	arg1	component					1042:1050	polyamine component	1032:1050	polyamine component	1032:1050	The respiratory isoprenoid quinone and polyamine component were identified as MK-7 and sym-homospermidine, respectively.
31165292	0	23	theme	oligotrophus	13:24	arg1	sp					26:27	Hymenobacter oligotrophus sp	0:27	Hymenobacter oligotrophus sp.	0:28	Hymenobacter oligotrophus sp.
31165292	4	24	theme	gene	424:427	arg1	analysis					438:445	16S rRNA gene sequence analysis	415:445	16S rRNA gene sequence analysis	415:445	On the basis of 16S rRNA gene sequence analysis, strain sh-6T belongs to the genus Hymenobacter and is closely related to Hymenobacter deserti ZLB-3T (95.05%), Hymenobacter paludis KBP-30T (94.96%), Hymenobacter coalescens WW84T (94.04%), Hymenobacter gummosus ANT-18T (93.38%), Hymenobacter ocellatus Myx2105T (93.70%), Hymenobacter jeollabukensis 1-3-3-8T (93.48%) and Hymenobacter koreensis GYR3077T (93.21%).
31165292	6	25	theme	isoprenoid	1009:1018	arg1	sym-homospermidine					1080:1097	sym-homospermidine	1080:1097	sym-homospermidine	1080:1097	The respiratory isoprenoid quinone and polyamine component were identified as MK-7 and sym-homospermidine, respectively.
31165292	6	25	theme	isoprenoid	1009:1018	arg1	MK-7					1071:1074	MK-7	1071:1074	MK-7	1071:1074	The respiratory isoprenoid quinone and polyamine component were identified as MK-7 and sym-homospermidine, respectively.
31165292	6	25	theme	isoprenoid	1009:1018	arg1	quinone					1020:1026	The respiratory isoprenoid quinone	993:1026	The respiratory isoprenoid quinone	993:1026	The respiratory isoprenoid quinone and polyamine component were identified as MK-7 and sym-homospermidine, respectively.
31165292	6	25	theme	isoprenoid	1009:1018	arg1	component					1042:1050	polyamine component	1032:1050	polyamine component	1032:1050	The respiratory isoprenoid quinone and polyamine component were identified as MK-7 and sym-homospermidine, respectively.
31165292	10	26	theme	genus	1463:1467	arg1	Hymenobacter					1469:1480	the genus Hymenobacter	1459:1480	the genus Hymenobacter	1459:1480	On the basis of the evidence presented in this study, a novel species of the genus Hymenobacter, Hymenobacter oligotrophus sp.
31165292	10	26	theme	genus	1463:1467	arg1	sp					1509:1510	Hymenobacter oligotrophus sp	1483:1510	Hymenobacter oligotrophus sp	1483:1510	On the basis of the evidence presented in this study, a novel species of the genus Hymenobacter, Hymenobacter oligotrophus sp.
31165292	0	27	theme	Hymenobacter	0:11	arg1	sp					26:27	Hymenobacter oligotrophus sp	0:27	Hymenobacter oligotrophus sp.	0:28	Hymenobacter oligotrophus sp.
31165292	11	28	theme	type	1541:1544	arg1	62345T					1587:1592	= CCTCC AB 2016064T = KCTC 62345T	1560:1592	= CCTCC AB 2016064T = KCTC 62345T	1560:1592	nov., is proposed, with the type strain sh-6T (= CCTCC AB 2016064T = KCTC 62345T).
31165292	11	28	theme	type	1541:1544	arg1	sh-6T					1553:1557	the type strain sh-6T	1537:1557	the type strain sh-6T (= CCTCC AB 2016064T = KCTC 62345T)	1537:1593	nov., is proposed, with the type strain sh-6T (= CCTCC AB 2016064T = KCTC 62345T).
31165292	6	29	theme	respiratory	997:1007	arg1	sym-homospermidine					1080:1097	sym-homospermidine	1080:1097	sym-homospermidine	1080:1097	The respiratory isoprenoid quinone and polyamine component were identified as MK-7 and sym-homospermidine, respectively.
31165292	6	29	theme	respiratory	997:1007	arg1	MK-7					1071:1074	MK-7	1071:1074	MK-7	1071:1074	The respiratory isoprenoid quinone and polyamine component were identified as MK-7 and sym-homospermidine, respectively.
31165292	6	29	theme	respiratory	997:1007	arg1	quinone					1020:1026	The respiratory isoprenoid quinone	993:1026	The respiratory isoprenoid quinone	993:1026	The respiratory isoprenoid quinone and polyamine component were identified as MK-7 and sym-homospermidine, respectively.
31165292	6	29	theme	respiratory	997:1007	arg1	component					1042:1050	polyamine component	1032:1050	polyamine component	1032:1050	The respiratory isoprenoid quinone and polyamine component were identified as MK-7 and sym-homospermidine, respectively.
31165292	3	30	theme	R2A	387:389	arg1	plates					391:396	R2A plates	387:396	R2A plates	387:396	Its optimum growth temperature was determined to be 28 °C in the absence of NaCl on R2A plates.
31165292	7	31	theme	iso-C17:0	1223:1231	arg1	3-OH					1233:1236	iso-C17:0 3-OH	1223:1236	iso-C17:0 3-OH	1223:1236	The major cellular fatty acids identified as iso-C15:0, summed feature 4 (iso-C17:1 I/anteiso B), iso-C16:0, iso-C17:0 3-OH and iso-C17:0.
31165292	5	32	theme	H.	865:866	arg1	ANT-18T					877:883	H. gummosus ANT-18T	865:883	H. gummosus ANT-18T	865:883	Comparison of the genome of strain sh-6T and that of H. gummosus ANT-18T gave digital DNA-DNA hybridization and Average Nucleotide Identity values of 20.6% and 78.4%, respectively.
31165292	7	33	theme	summed	1170:1175	arg1	B					1208:1208	iso-C17:1 I/anteiso B	1188:1208	iso-C17:1 I/anteiso B	1188:1208	The major cellular fatty acids identified as iso-C15:0, summed feature 4 (iso-C17:1 I/anteiso B), iso-C16:0, iso-C17:0 3-OH and iso-C17:0.
31165292	7	33	theme	summed	1170:1175	arg1	feature					1177:1183	summed feature 4	1170:1185	summed feature 4 (iso-C17:1 I/anteiso B)	1170:1209	The major cellular fatty acids identified as iso-C15:0, summed feature 4 (iso-C17:1 I/anteiso B), iso-C16:0, iso-C17:0 3-OH and iso-C17:0.
31165292	2	34	theme	contaminated	247:258	arg1	plate					264:268	a contaminated R2A plate	245:268	a contaminated R2A plate in the laboratory	245:286	A taxonomic study of a Gram-stain negative, rod-shaped, motile, asporogenous, catalase- and oxidase-positive bacterium, sh-6T, forming pink-red colonies, isolated from a contaminated R2A plate in the laboratory was performed.
31165292	2	35	theme	catalase-	155:163	arg1	sh-6T					197:201	sh-6T	197:201	sh-6T	197:201	A taxonomic study of a Gram-stain negative, rod-shaped, motile, asporogenous, catalase- and oxidase-positive bacterium, sh-6T, forming pink-red colonies, isolated from a contaminated R2A plate in the laboratory was performed.
31165292	2	35	theme	catalase-	155:163	arg1	bacterium					186:194	a Gram-stain negative, rod-shaped, motile, asporogenous, catalase- and oxidase-positive bacterium	98:194	a Gram-stain negative, rod-shaped, motile, asporogenous, catalase- and oxidase-positive bacterium	98:194	A taxonomic study of a Gram-stain negative, rod-shaped, motile, asporogenous, catalase- and oxidase-positive bacterium, sh-6T, forming pink-red colonies, isolated from a contaminated R2A plate in the laboratory was performed.
31165292	5	36	theme	%	966:966	arg1	values					952:957	digital DNA-DNA hybridization and Average Nucleotide Identity values	890:957	digital DNA-DNA hybridization and Average Nucleotide Identity values of 20.6% and 78.4%, respectively	890:990	Comparison of the genome of strain sh-6T and that of H. gummosus ANT-18T gave digital DNA-DNA hybridization and Average Nucleotide Identity values of 20.6% and 78.4%, respectively.
31165292	5	37	theme	Nucleotide	932:941	arg1	Identity					943:950	Average Nucleotide Identity	924:950	Average Nucleotide Identity	924:950	Comparison of the genome of strain sh-6T and that of H. gummosus ANT-18T gave digital DNA-DNA hybridization and Average Nucleotide Identity values of 20.6% and 78.4%, respectively.
31165292	2	38	theme	pink-red	212:219	arg1	colonies					221:228	pink-red colonies	212:228	pink-red colonies	212:228	A taxonomic study of a Gram-stain negative, rod-shaped, motile, asporogenous, catalase- and oxidase-positive bacterium, sh-6T, forming pink-red colonies, isolated from a contaminated R2A plate in the laboratory was performed.
31165292	10	39	theme	oligotrophus	1496:1507	arg1	Hymenobacter					1469:1480	the genus Hymenobacter	1459:1480	the genus Hymenobacter	1459:1480	On the basis of the evidence presented in this study, a novel species of the genus Hymenobacter, Hymenobacter oligotrophus sp.
31165292	10	39	theme	oligotrophus	1496:1507	arg1	sp					1509:1510	Hymenobacter oligotrophus sp	1483:1510	Hymenobacter oligotrophus sp	1483:1510	On the basis of the evidence presented in this study, a novel species of the genus Hymenobacter, Hymenobacter oligotrophus sp.
31165292	3	40	theme	NaCl	379:382	arg1	absence					368:374	the absence	364:374	the absence of NaCl on R2A plates	364:396	Its optimum growth temperature was determined to be 28 °C in the absence of NaCl on R2A plates.
31165292	10	41	dep	evidence	1406:1413	arg1	the					1389:1391	the	1389:1391	the	1389:1391	On the basis of the evidence presented in this study, a novel species of the genus Hymenobacter, Hymenobacter oligotrophus sp.
31165292	10	41	dep	evidence	1406:1413	arg1	basis					1393:1397	basis	1393:1397	basis	1393:1397	On the basis of the evidence presented in this study, a novel species of the genus Hymenobacter, Hymenobacter oligotrophus sp.
31165292	2	42	theme	asporogenous	141:152	arg1	sh-6T					197:201	sh-6T	197:201	sh-6T	197:201	A taxonomic study of a Gram-stain negative, rod-shaped, motile, asporogenous, catalase- and oxidase-positive bacterium, sh-6T, forming pink-red colonies, isolated from a contaminated R2A plate in the laboratory was performed.
31165292	2	42	theme	asporogenous	141:152	arg1	bacterium					186:194	a Gram-stain negative, rod-shaped, motile, asporogenous, catalase- and oxidase-positive bacterium	98:194	a Gram-stain negative, rod-shaped, motile, asporogenous, catalase- and oxidase-positive bacterium	98:194	A taxonomic study of a Gram-stain negative, rod-shaped, motile, asporogenous, catalase- and oxidase-positive bacterium, sh-6T, forming pink-red colonies, isolated from a contaminated R2A plate in the laboratory was performed.
31165292	5	43	theme	gummosus	868:875	arg1	ANT-18T					877:883	H. gummosus ANT-18T	865:883	H. gummosus ANT-18T	865:883	Comparison of the genome of strain sh-6T and that of H. gummosus ANT-18T gave digital DNA-DNA hybridization and Average Nucleotide Identity values of 20.6% and 78.4%, respectively.
31165292	8	44	dep	sh-6T	1285:1289	arg1	determined					1291:1300	determined	1291:1300	sh-6T determined to be phosphatidylethanolamine	1285:1331	The major polar lipid of strain sh-6T determined to be phosphatidylethanolamine.
31165292	10	45	theme	novel	1442:1446	arg1	species					1448:1454	a novel species	1440:1454	a novel species of the genus Hymenobacter, Hymenobacter oligotrophus sp	1440:1510	On the basis of the evidence presented in this study, a novel species of the genus Hymenobacter, Hymenobacter oligotrophus sp.
31165292	2	46	theme	taxonomic	79:87	arg1	study					89:93	A taxonomic study	77:93	A taxonomic study of a Gram-stain negative, rod-shaped, motile, asporogenous, catalase- and oxidase-positive bacterium, sh-6T, forming pink-red colonies, isolated from a contaminated R2A plate in the laboratory	77:286	A taxonomic study of a Gram-stain negative, rod-shaped, motile, asporogenous, catalase- and oxidase-positive bacterium, sh-6T, forming pink-red colonies, isolated from a contaminated R2A plate in the laboratory was performed.
31165292	5	47	theme	genome	830:835	arg1	Comparison					812:821	Comparison	812:821	Comparison of the genome of strain sh-6T	812:851	Comparison of the genome of strain sh-6T and that of H. gummosus ANT-18T gave digital DNA-DNA hybridization and Average Nucleotide Identity values of 20.6% and 78.4%, respectively.
31165292	4	48	theme	sequence	429:436	arg1	analysis					438:445	16S rRNA gene sequence analysis	415:445	16S rRNA gene sequence analysis	415:445	On the basis of 16S rRNA gene sequence analysis, strain sh-6T belongs to the genus Hymenobacter and is closely related to Hymenobacter deserti ZLB-3T (95.05%), Hymenobacter paludis KBP-30T (94.96%), Hymenobacter coalescens WW84T (94.04%), Hymenobacter gummosus ANT-18T (93.38%), Hymenobacter ocellatus Myx2105T (93.70%), Hymenobacter jeollabukensis 1-3-3-8T (93.48%) and Hymenobacter koreensis GYR3077T (93.21%).
31165292	9	49	theme	G+C	1342:1344	arg1	content					1346:1352	The DNA G+C content	1334:1352	The DNA G+C content	1334:1352	The DNA G+C content was determined to be 60.5 mol%.
31165292	9	49	theme	G+C	1342:1344	arg1	%					1383:1383	60.5 mol%	1375:1383	60.5 mol%	1375:1383	The DNA G+C content was determined to be 60.5 mol%.
31165292	4	50	theme	genus	476:480	arg1	Hymenobacter					482:493	the genus Hymenobacter	472:493	the genus Hymenobacter	472:493	On the basis of 16S rRNA gene sequence analysis, strain sh-6T belongs to the genus Hymenobacter and is closely related to Hymenobacter deserti ZLB-3T (95.05%), Hymenobacter paludis KBP-30T (94.96%), Hymenobacter coalescens WW84T (94.04%), Hymenobacter gummosus ANT-18T (93.38%), Hymenobacter ocellatus Myx2105T (93.70%), Hymenobacter jeollabukensis 1-3-3-8T (93.48%) and Hymenobacter koreensis GYR3077T (93.21%).
31165292	5	51	theme	digital	890:896	arg1	hybridization					906:918	digital DNA-DNA hybridization	890:918	digital DNA-DNA hybridization	890:918	Comparison of the genome of strain sh-6T and that of H. gummosus ANT-18T gave digital DNA-DNA hybridization and Average Nucleotide Identity values of 20.6% and 78.4%, respectively.
31165292	5	52	theme	DNA-DNA	898:904	arg1	hybridization					906:918	digital DNA-DNA hybridization	890:918	digital DNA-DNA hybridization	890:918	Comparison of the genome of strain sh-6T and that of H. gummosus ANT-18T gave digital DNA-DNA hybridization and Average Nucleotide Identity values of 20.6% and 78.4%, respectively.
31165292	4	53	dep	Hymenobacter	638:649	arg1	gummosus					651:658	Hymenobacter gummosus ANT-18T (93.38%)	638:675	Hymenobacter gummosus ANT-18T (93.38%)	638:675	On the basis of 16S rRNA gene sequence analysis, strain sh-6T belongs to the genus Hymenobacter and is closely related to Hymenobacter deserti ZLB-3T (95.05%), Hymenobacter paludis KBP-30T (94.96%), Hymenobacter coalescens WW84T (94.04%), Hymenobacter gummosus ANT-18T (93.38%), Hymenobacter ocellatus Myx2105T (93.70%), Hymenobacter jeollabukensis 1-3-3-8T (93.48%) and Hymenobacter koreensis GYR3077T (93.21%).
31165292	4	53	dep	Hymenobacter	638:649	arg1	ANT-18T					660:666	ANT-18T	660:666	ANT-18T	660:666	On the basis of 16S rRNA gene sequence analysis, strain sh-6T belongs to the genus Hymenobacter and is closely related to Hymenobacter deserti ZLB-3T (95.05%), Hymenobacter paludis KBP-30T (94.96%), Hymenobacter coalescens WW84T (94.04%), Hymenobacter gummosus ANT-18T (93.38%), Hymenobacter ocellatus Myx2105T (93.70%), Hymenobacter jeollabukensis 1-3-3-8T (93.48%) and Hymenobacter koreensis GYR3077T (93.21%).
31165292	4	53	dep	Hymenobacter	638:649	arg1	%					674:674	93.38%	669:674	93.38%	669:674	On the basis of 16S rRNA gene sequence analysis, strain sh-6T belongs to the genus Hymenobacter and is closely related to Hymenobacter deserti ZLB-3T (95.05%), Hymenobacter paludis KBP-30T (94.96%), Hymenobacter coalescens WW84T (94.04%), Hymenobacter gummosus ANT-18T (93.38%), Hymenobacter ocellatus Myx2105T (93.70%), Hymenobacter jeollabukensis 1-3-3-8T (93.48%) and Hymenobacter koreensis GYR3077T (93.21%).
31165292	4	54	dep	analysis	438:445	arg1	the					402:404	the	402:404	the	402:404	On the basis of 16S rRNA gene sequence analysis, strain sh-6T belongs to the genus Hymenobacter and is closely related to Hymenobacter deserti ZLB-3T (95.05%), Hymenobacter paludis KBP-30T (94.96%), Hymenobacter coalescens WW84T (94.04%), Hymenobacter gummosus ANT-18T (93.38%), Hymenobacter ocellatus Myx2105T (93.70%), Hymenobacter jeollabukensis 1-3-3-8T (93.48%) and Hymenobacter koreensis GYR3077T (93.21%).
31165292	4	54	dep	analysis	438:445	arg1	basis					406:410	basis	406:410	basis	406:410	On the basis of 16S rRNA gene sequence analysis, strain sh-6T belongs to the genus Hymenobacter and is closely related to Hymenobacter deserti ZLB-3T (95.05%), Hymenobacter paludis KBP-30T (94.96%), Hymenobacter coalescens WW84T (94.04%), Hymenobacter gummosus ANT-18T (93.38%), Hymenobacter ocellatus Myx2105T (93.70%), Hymenobacter jeollabukensis 1-3-3-8T (93.48%) and Hymenobacter koreensis GYR3077T (93.21%).
31165292	2	55	theme	bacterium	186:194	arg1	study					89:93	A taxonomic study	77:93	A taxonomic study of a Gram-stain negative, rod-shaped, motile, asporogenous, catalase- and oxidase-positive bacterium, sh-6T, forming pink-red colonies, isolated from a contaminated R2A plate in the laboratory	77:286	A taxonomic study of a Gram-stain negative, rod-shaped, motile, asporogenous, catalase- and oxidase-positive bacterium, sh-6T, forming pink-red colonies, isolated from a contaminated R2A plate in the laboratory was performed.
31165292	5	56	theme	sh-6T	847:851	arg1	genome					830:835	the genome	826:835	the genome of strain sh-6T	826:851	Comparison of the genome of strain sh-6T and that of H. gummosus ANT-18T gave digital DNA-DNA hybridization and Average Nucleotide Identity values of 20.6% and 78.4%, respectively.
31165292	7	57	theme	fatty	1133:1137	arg1	acids					1139:1143	The major cellular fatty acids	1114:1143	The major cellular fatty acids	1114:1143	The major cellular fatty acids identified as iso-C15:0, summed feature 4 (iso-C17:1 I/anteiso B), iso-C16:0, iso-C17:0 3-OH and iso-C17:0.
31165292	2	58	attach	isolated	231:238	arg2	sh-6T					197:201	sh-6T	197:201	sh-6T	197:201	A taxonomic study of a Gram-stain negative, rod-shaped, motile, asporogenous, catalase- and oxidase-positive bacterium, sh-6T, forming pink-red colonies, isolated from a contaminated R2A plate in the laboratory was performed.
31165292	2	58	attach	isolated	231:238	arg1	plate					264:268	a contaminated R2A plate	245:268	a contaminated R2A plate in the laboratory	245:286	A taxonomic study of a Gram-stain negative, rod-shaped, motile, asporogenous, catalase- and oxidase-positive bacterium, sh-6T, forming pink-red colonies, isolated from a contaminated R2A plate in the laboratory was performed.
31165292	2	58	attach	isolated	231:238	arg2	bacterium					186:194	a Gram-stain negative, rod-shaped, motile, asporogenous, catalase- and oxidase-positive bacterium	98:194	a Gram-stain negative, rod-shaped, motile, asporogenous, catalase- and oxidase-positive bacterium	98:194	A taxonomic study of a Gram-stain negative, rod-shaped, motile, asporogenous, catalase- and oxidase-positive bacterium, sh-6T, forming pink-red colonies, isolated from a contaminated R2A plate in the laboratory was performed.
31165292	5	59	theme	strain	840:845	arg1	sh-6T					847:851	strain sh-6T	840:851	strain sh-6T	840:851	Comparison of the genome of strain sh-6T and that of H. gummosus ANT-18T gave digital DNA-DNA hybridization and Average Nucleotide Identity values of 20.6% and 78.4%, respectively.
31165292	2	60	theme	oxidase-positive	169:184	arg1	sh-6T					197:201	sh-6T	197:201	sh-6T	197:201	A taxonomic study of a Gram-stain negative, rod-shaped, motile, asporogenous, catalase- and oxidase-positive bacterium, sh-6T, forming pink-red colonies, isolated from a contaminated R2A plate in the laboratory was performed.
31165292	2	60	theme	oxidase-positive	169:184	arg1	bacterium					186:194	a Gram-stain negative, rod-shaped, motile, asporogenous, catalase- and oxidase-positive bacterium	98:194	a Gram-stain negative, rod-shaped, motile, asporogenous, catalase- and oxidase-positive bacterium	98:194	A taxonomic study of a Gram-stain negative, rod-shaped, motile, asporogenous, catalase- and oxidase-positive bacterium, sh-6T, forming pink-red colonies, isolated from a contaminated R2A plate in the laboratory was performed.
31165292	8	61	theme	polar	1263:1267	arg1	lipid					1269:1273	The major polar lipid	1253:1273	The major polar lipid of strain	1253:1283	The major polar lipid of strain sh-6T determined to be phosphatidylethanolamine.
31165292	10	62	theme	Hymenobacter	1483:1494	arg1	Hymenobacter					1469:1480	the genus Hymenobacter	1459:1480	the genus Hymenobacter	1459:1480	On the basis of the evidence presented in this study, a novel species of the genus Hymenobacter, Hymenobacter oligotrophus sp.
31165292	10	62	theme	Hymenobacter	1483:1494	arg1	sp					1509:1510	Hymenobacter oligotrophus sp	1483:1510	Hymenobacter oligotrophus sp	1483:1510	On the basis of the evidence presented in this study, a novel species of the genus Hymenobacter, Hymenobacter oligotrophus sp.
31165292	2	63	from	plate	264:268	arg1	laboratory					277:286	the laboratory	273:286	the laboratory	273:286	A taxonomic study of a Gram-stain negative, rod-shaped, motile, asporogenous, catalase- and oxidase-positive bacterium, sh-6T, forming pink-red colonies, isolated from a contaminated R2A plate in the laboratory was performed.
31165292	11	64	theme	= CCTCC	1560:1566	arg1	62345T					1587:1592	= CCTCC AB 2016064T = KCTC 62345T	1560:1592	= CCTCC AB 2016064T = KCTC 62345T	1560:1592	nov., is proposed, with the type strain sh-6T (= CCTCC AB 2016064T = KCTC 62345T).
31165292	11	64	theme	= CCTCC	1560:1566	arg1	sh-6T					1553:1557	the type strain sh-6T	1537:1557	the type strain sh-6T (= CCTCC AB 2016064T = KCTC 62345T)	1537:1593	nov., is proposed, with the type strain sh-6T (= CCTCC AB 2016064T = KCTC 62345T).
31165292	9	65	theme	DNA	1338:1340	arg1	content					1346:1352	The DNA G+C content	1334:1352	The DNA G+C content	1334:1352	The DNA G+C content was determined to be 60.5 mol%.
31165292	9	65	theme	DNA	1338:1340	arg1	%					1383:1383	60.5 mol%	1375:1383	60.5 mol%	1375:1383	The DNA G+C content was determined to be 60.5 mol%.
31165292	8	66	theme	strain	1278:1283	arg1	lipid					1269:1273	The major polar lipid	1253:1273	The major polar lipid of strain	1253:1283	The major polar lipid of strain sh-6T determined to be phosphatidylethanolamine.
31165292	7	67	theme	major	1118:1122	arg1	acids					1139:1143	The major cellular fatty acids	1114:1143	The major cellular fatty acids	1114:1143	The major cellular fatty acids identified as iso-C15:0, summed feature 4 (iso-C17:1 I/anteiso B), iso-C16:0, iso-C17:0 3-OH and iso-C17:0.
31165292	7	68	theme	I/anteiso	1198:1206	arg1	B					1208:1208	iso-C17:1 I/anteiso B	1188:1208	iso-C17:1 I/anteiso B	1188:1208	The major cellular fatty acids identified as iso-C15:0, summed feature 4 (iso-C17:1 I/anteiso B), iso-C16:0, iso-C17:0 3-OH and iso-C17:0.
31165292	7	68	theme	I/anteiso	1198:1206	arg1	feature					1177:1183	summed feature 4	1170:1185	summed feature 4 (iso-C17:1 I/anteiso B)	1170:1209	The major cellular fatty acids identified as iso-C15:0, summed feature 4 (iso-C17:1 I/anteiso B), iso-C16:0, iso-C17:0 3-OH and iso-C17:0.
31165292	11	69	theme	strain	1546:1551	arg1	62345T					1587:1592	= CCTCC AB 2016064T = KCTC 62345T	1560:1592	= CCTCC AB 2016064T = KCTC 62345T	1560:1592	nov., is proposed, with the type strain sh-6T (= CCTCC AB 2016064T = KCTC 62345T).
31165292	11	69	theme	strain	1546:1551	arg1	sh-6T					1553:1557	the type strain sh-6T	1537:1557	the type strain sh-6T (= CCTCC AB 2016064T = KCTC 62345T)	1537:1593	nov., is proposed, with the type strain sh-6T (= CCTCC AB 2016064T = KCTC 62345T).
31165292	3	70	theme	optimum	307:313	arg1	temperature					322:332	Its optimum growth temperature	303:332	Its optimum growth temperature	303:332	Its optimum growth temperature was determined to be 28 °C in the absence of NaCl on R2A plates.
31165292	2	71	theme	negative	111:118	arg1	sh-6T					197:201	sh-6T	197:201	sh-6T	197:201	A taxonomic study of a Gram-stain negative, rod-shaped, motile, asporogenous, catalase- and oxidase-positive bacterium, sh-6T, forming pink-red colonies, isolated from a contaminated R2A plate in the laboratory was performed.
31165292	2	71	theme	negative	111:118	arg1	bacterium					186:194	a Gram-stain negative, rod-shaped, motile, asporogenous, catalase- and oxidase-positive bacterium	98:194	a Gram-stain negative, rod-shaped, motile, asporogenous, catalase- and oxidase-positive bacterium	98:194	A taxonomic study of a Gram-stain negative, rod-shaped, motile, asporogenous, catalase- and oxidase-positive bacterium, sh-6T, forming pink-red colonies, isolated from a contaminated R2A plate in the laboratory was performed.
31165292	7	72	theme	cellular	1124:1131	arg1	acids					1139:1143	The major cellular fatty acids	1114:1143	The major cellular fatty acids	1114:1143	The major cellular fatty acids identified as iso-C15:0, summed feature 4 (iso-C17:1 I/anteiso B), iso-C16:0, iso-C17:0 3-OH and iso-C17:0.
31359753	5	0	from	profile	1545:1551	arg1	absent					1525:1530	absent	1525:1530	absent	1525:1530	The ability of the GCIB to generate increased higher mass signals from biological samples allowed intact lipid A (m/z 1796) to be detected on the bacteria and, despite a 40 keV impact energy, depth profiled through the bacterial envelope along with other high mass ions including species at m/z 1820 and 2428, attributed to ECACYC, that were only observed below the surface of the bacteria and were notably absent in the depth profile of the lpp mutant.
31359753	1	1	theme	CO2	254:256	arg1	GCIB					284:287	GCIB	284:287	GCIB	284:287	Time-of-flight secondary ion mass spectrometry (ToF-SIMS) using a (CO2)6k+ gas cluster ion beam (GCIB) was used to analyze Escherichia coli mutants previously identified as having impaired plasmid transfer capability related to the spread of antibiotic resistance.
31359753	1	1	theme	CO2	254:256	arg1	beam					278:281	a (CO2)6k+ gas cluster ion beam	251:281	a (CO2)6k+ gas cluster ion beam (GCIB)	251:288	Time-of-flight secondary ion mass spectrometry (ToF-SIMS) using a (CO2)6k+ gas cluster ion beam (GCIB) was used to analyze Escherichia coli mutants previously identified as having impaired plasmid transfer capability related to the spread of antibiotic resistance.
31359753	1	2	theme	6k+	258:260	arg1	GCIB					284:287	GCIB	284:287	GCIB	284:287	Time-of-flight secondary ion mass spectrometry (ToF-SIMS) using a (CO2)6k+ gas cluster ion beam (GCIB) was used to analyze Escherichia coli mutants previously identified as having impaired plasmid transfer capability related to the spread of antibiotic resistance.
31359753	1	2	theme	6k+	258:260	arg1	beam					278:281	a (CO2)6k+ gas cluster ion beam	251:281	a (CO2)6k+ gas cluster ion beam (GCIB)	251:288	Time-of-flight secondary ion mass spectrometry (ToF-SIMS) using a (CO2)6k+ gas cluster ion beam (GCIB) was used to analyze Escherichia coli mutants previously identified as having impaired plasmid transfer capability related to the spread of antibiotic resistance.
31359753	6	3	theme	intact	1739:1744	arg1	molecules					1746:1754	intact molecules	1739:1754	intact molecules within the bacterial envelope	1739:1784	The analysis provides new insights into the action of the specific pathways targeted in this study and paves the way for whole new avenues for the characterization of intact molecules within the bacterial envelope.
31359753	1	4	theme	resistance	440:449	arg1	spread					419:424	the spread	415:424	the spread of antibiotic resistance	415:449	Time-of-flight secondary ion mass spectrometry (ToF-SIMS) using a (CO2)6k+ gas cluster ion beam (GCIB) was used to analyze Escherichia coli mutants previously identified as having impaired plasmid transfer capability related to the spread of antibiotic resistance.
31359753	5	5	theme	bacteria	1499:1506	arg1	surface					1484:1490	the surface	1480:1490	the surface of the bacteria	1480:1506	The ability of the GCIB to generate increased higher mass signals from biological samples allowed intact lipid A (m/z 1796) to be detected on the bacteria and, despite a 40 keV impact energy, depth profiled through the bacterial envelope along with other high mass ions including species at m/z 1820 and 2428, attributed to ECACYC, that were only observed below the surface of the bacteria and were notably absent in the depth profile of the lpp mutant.
31359753	1	6	theme	secondary	202:210	arg1	ToF-SIMS					235:242	ToF-SIMS	235:242	ToF-SIMS	235:242	Time-of-flight secondary ion mass spectrometry (ToF-SIMS) using a (CO2)6k+ gas cluster ion beam (GCIB) was used to analyze Escherichia coli mutants previously identified as having impaired plasmid transfer capability related to the spread of antibiotic resistance.
31359753	1	6	theme	secondary	202:210	arg1	spectrometry					221:232	Time-of-flight secondary ion mass spectrometry	187:232	Time-of-flight secondary ion mass spectrometry (ToF-SIMS) using a (CO2)6k+ gas cluster ion beam (GCIB)	187:288	Time-of-flight secondary ion mass spectrometry (ToF-SIMS) using a (CO2)6k+ gas cluster ion beam (GCIB) was used to analyze Escherichia coli mutants previously identified as having impaired plasmid transfer capability related to the spread of antibiotic resistance.
31359753	2	7	theme	bacterial	525:533	arg1	composition					544:554	the bacterial envelope composition	521:554	the bacterial envelope composition	521:554	The subset of mutants selected were expected to result in changes in the bacterial envelope composition through the deletion of genes encoding for FabF, DapF, and Lpp, where the surface sensitivity of ToF-SIMS can be most useful.
31359753	5	8	from	samples	1200:1206	arg1	signals					1176:1182	higher mass signals	1164:1182	higher mass signals from biological samples allowed intact lipid A (m/z 1796) to be detected on the bacteria and, despite a 40 keV impact energy, depth	1164:1314	The ability of the GCIB to generate increased higher mass signals from biological samples allowed intact lipid A (m/z 1796) to be detected on the bacteria and, despite a 40 keV impact energy, depth profiled through the bacterial envelope along with other high mass ions including species at m/z 1820 and 2428, attributed to ECACYC, that were only observed below the surface of the bacteria and were notably absent in the depth profile of the lpp mutant.
31359753	0	9	theme	Plasmid	105:111	arg1	Transfer					113:120	Impaired Plasmid Transfer	96:120	Impaired Plasmid Transfer	96:120	Chemical Changes On, and Through, The Bacterial Envelope in Escherichia coli Mutants Exhibiting Impaired Plasmid Transfer Identified Using Time-of-Flight Secondary Ion Mass Spectrometry.
31359753	1	10	theme	mass	216:219	arg1	ToF-SIMS					235:242	ToF-SIMS	235:242	ToF-SIMS	235:242	Time-of-flight secondary ion mass spectrometry (ToF-SIMS) using a (CO2)6k+ gas cluster ion beam (GCIB) was used to analyze Escherichia coli mutants previously identified as having impaired plasmid transfer capability related to the spread of antibiotic resistance.
31359753	1	10	theme	mass	216:219	arg1	spectrometry					221:232	Time-of-flight secondary ion mass spectrometry	187:232	Time-of-flight secondary ion mass spectrometry (ToF-SIMS) using a (CO2)6k+ gas cluster ion beam (GCIB)	187:288	Time-of-flight secondary ion mass spectrometry (ToF-SIMS) using a (CO2)6k+ gas cluster ion beam (GCIB) was used to analyze Escherichia coli mutants previously identified as having impaired plasmid transfer capability related to the spread of antibiotic resistance.
31359753	6	11	theme	new	1699:1701	arg1	avenues					1703:1709	whole new avenues	1693:1709	whole new avenues for the characterization of intact molecules within the bacterial envelope	1693:1784	The analysis provides new insights into the action of the specific pathways targeted in this study and paves the way for whole new avenues for the characterization of intact molecules within the bacterial envelope.
31359753	1	12	theme	impaired	367:374	arg1	capability					393:402	impaired plasmid transfer capability	367:402	impaired plasmid transfer capability related to the spread of antibiotic resistance	367:449	Time-of-flight secondary ion mass spectrometry (ToF-SIMS) using a (CO2)6k+ gas cluster ion beam (GCIB) was used to analyze Escherichia coli mutants previously identified as having impaired plasmid transfer capability related to the spread of antibiotic resistance.
31359753	5	13	dep	generate	1145:1152	arg1	increased					1154:1162	increased	1154:1162	to generate increased higher mass signals from biological samples allowed intact lipid A (m/z 1796) to be detected on the bacteria and, despite a 40 keV impact energy, depth	1142:1314	The ability of the GCIB to generate increased higher mass signals from biological samples allowed intact lipid A (m/z 1796) to be detected on the bacteria and, despite a 40 keV impact energy, depth profiled through the bacterial envelope along with other high mass ions including species at m/z 1820 and 2428, attributed to ECACYC, that were only observed below the surface of the bacteria and were notably absent in the depth profile of the lpp mutant.
31359753	4	14	theme	cyclopropane	1094:1105	arg1	formation					1107:1115	cyclopropane formation	1094:1115	cyclopropane formation	1094:1115	Significant changes in chemical composition were observed, including a surprising loss of cyclopropanated fatty acids in the fabF mutant where FabF is associated with the elongation of FA(16:1) to FA(18:1) and not cyclopropane formation.
31359753	3	15	theme	bacteria	712:719	arg1	arrays					694:699	arrays	694:699	arrays of spotted bacteria	694:719	Analysis of arrays of spotted bacteria allowed changes in the lipid composition of the bacteria to be elucidated using multivariate analysis and confirmed through imaging of individual ion signals.
31359753	0	16	theme	Time-of-Flight	139:152	arg1	Spectrometry					173:184	Time-of-Flight Secondary Ion Mass Spectrometry	139:184	Time-of-Flight Secondary Ion Mass Spectrometry	139:184	Chemical Changes On, and Through, The Bacterial Envelope in Escherichia coli Mutants Exhibiting Impaired Plasmid Transfer Identified Using Time-of-Flight Secondary Ion Mass Spectrometry.
31359753	1	17	theme	transfer	384:391	arg1	capability					393:402	impaired plasmid transfer capability	367:402	impaired plasmid transfer capability related to the spread of antibiotic resistance	367:449	Time-of-flight secondary ion mass spectrometry (ToF-SIMS) using a (CO2)6k+ gas cluster ion beam (GCIB) was used to analyze Escherichia coli mutants previously identified as having impaired plasmid transfer capability related to the spread of antibiotic resistance.
31359753	3	18	theme	multivariate	801:812	arg1	analysis					814:821	multivariate analysis	801:821	multivariate analysis	801:821	Analysis of arrays of spotted bacteria allowed changes in the lipid composition of the bacteria to be elucidated using multivariate analysis and confirmed through imaging of individual ion signals.
31359753	1	19	used	used	294:297	arg2	ToF-SIMS					235:242	ToF-SIMS	235:242	ToF-SIMS	235:242	Time-of-flight secondary ion mass spectrometry (ToF-SIMS) using a (CO2)6k+ gas cluster ion beam (GCIB) was used to analyze Escherichia coli mutants previously identified as having impaired plasmid transfer capability related to the spread of antibiotic resistance.
31359753	1	19	used	used	294:297	arg2	spectrometry					221:232	Time-of-flight secondary ion mass spectrometry	187:232	Time-of-flight secondary ion mass spectrometry (ToF-SIMS) using a (CO2)6k+ gas cluster ion beam (GCIB)	187:288	Time-of-flight secondary ion mass spectrometry (ToF-SIMS) using a (CO2)6k+ gas cluster ion beam (GCIB) was used to analyze Escherichia coli mutants previously identified as having impaired plasmid transfer capability related to the spread of antibiotic resistance.
31359753	0	20	theme	Ion	164:166	arg1	Spectrometry					173:184	Time-of-Flight Secondary Ion Mass Spectrometry	139:184	Time-of-Flight Secondary Ion Mass Spectrometry	139:184	Chemical Changes On, and Through, The Bacterial Envelope in Escherichia coli Mutants Exhibiting Impaired Plasmid Transfer Identified Using Time-of-Flight Secondary Ion Mass Spectrometry.
31359753	1	21	theme	related	404:410	arg1	capability					393:402	impaired plasmid transfer capability	367:402	impaired plasmid transfer capability related to the spread of antibiotic resistance	367:449	Time-of-flight secondary ion mass spectrometry (ToF-SIMS) using a (CO2)6k+ gas cluster ion beam (GCIB) was used to analyze Escherichia coli mutants previously identified as having impaired plasmid transfer capability related to the spread of antibiotic resistance.
31359753	5	22	from	m/z	1409:1411	arg1	species					1398:1404	species	1398:1404	species	1398:1404	The ability of the GCIB to generate increased higher mass signals from biological samples allowed intact lipid A (m/z 1796) to be detected on the bacteria and, despite a 40 keV impact energy, depth profiled through the bacterial envelope along with other high mass ions including species at m/z 1820 and 2428, attributed to ECACYC, that were only observed below the surface of the bacteria and were notably absent in the depth profile of the lpp mutant.
31359753	6	23	theme	bacterial	1767:1775	arg1	envelope					1777:1784	the bacterial envelope	1763:1784	the bacterial envelope	1763:1784	The analysis provides new insights into the action of the specific pathways targeted in this study and paves the way for whole new avenues for the characterization of intact molecules within the bacterial envelope.
31359753	5	24	theme	lipid	1223:1227	arg1	m/z					1232:1234	m/z 1796	1232:1239	m/z 1796	1232:1239	The ability of the GCIB to generate increased higher mass signals from biological samples allowed intact lipid A (m/z 1796) to be detected on the bacteria and, despite a 40 keV impact energy, depth profiled through the bacterial envelope along with other high mass ions including species at m/z 1820 and 2428, attributed to ECACYC, that were only observed below the surface of the bacteria and were notably absent in the depth profile of the lpp mutant.
31359753	5	24	theme	lipid	1223:1227	arg1	A					1229:1229	intact lipid A	1216:1229	intact lipid A (m/z 1796)	1216:1240	The ability of the GCIB to generate increased higher mass signals from biological samples allowed intact lipid A (m/z 1796) to be detected on the bacteria and, despite a 40 keV impact energy, depth profiled through the bacterial envelope along with other high mass ions including species at m/z 1820 and 2428, attributed to ECACYC, that were only observed below the surface of the bacteria and were notably absent in the depth profile of the lpp mutant.
31359753	0	25	theme	Bacterial	38:46	arg1	Envelope					48:55	The Bacterial Envelope	34:55	The Bacterial Envelope	34:55	Chemical Changes On, and Through, The Bacterial Envelope in Escherichia coli Mutants Exhibiting Impaired Plasmid Transfer Identified Using Time-of-Flight Secondary Ion Mass Spectrometry.
31359753	2	26	theme	mutants	466:472	arg1	subset					456:461	The subset	452:461	The subset of mutants selected	452:481	The subset of mutants selected were expected to result in changes in the bacterial envelope composition through the deletion of genes encoding for FabF, DapF, and Lpp, where the surface sensitivity of ToF-SIMS can be most useful.
31359753	6	27	theme	new	1594:1596	arg1	insights					1598:1605	new insights	1594:1605	new insights into the action of the specific pathways targeted in this study	1594:1669	The analysis provides new insights into the action of the specific pathways targeted in this study and paves the way for whole new avenues for the characterization of intact molecules within the bacterial envelope.
31359753	5	28	theme	other	1367:1371	arg1	species					1398:1404	species	1398:1404	species	1398:1404	The ability of the GCIB to generate increased higher mass signals from biological samples allowed intact lipid A (m/z 1796) to be detected on the bacteria and, despite a 40 keV impact energy, depth profiled through the bacterial envelope along with other high mass ions including species at m/z 1820 and 2428, attributed to ECACYC, that were only observed below the surface of the bacteria and were notably absent in the depth profile of the lpp mutant.
31359753	5	28	theme	other	1367:1371	arg1	ions					1383:1386	other high mass ions	1367:1386	other high mass ions including species at m/z 1820 and 2428, attributed to ECACYC, that were only observed below the surface of the bacteria and were notably absent in the depth profile of the lpp mutant	1367:1569	The ability of the GCIB to generate increased higher mass signals from biological samples allowed intact lipid A (m/z 1796) to be detected on the bacteria and, despite a 40 keV impact energy, depth profiled through the bacterial envelope along with other high mass ions including species at m/z 1820 and 2428, attributed to ECACYC, that were only observed below the surface of the bacteria and were notably absent in the depth profile of the lpp mutant.
31359753	3	29	from	changes	729:735	arg1	composition					750:760	the lipid composition	740:760	the lipid composition of the bacteria to be elucidated using multivariate analysis and confirmed through imaging of individual ion signals	740:877	Analysis of arrays of spotted bacteria allowed changes in the lipid composition of the bacteria to be elucidated using multivariate analysis and confirmed through imaging of individual ion signals.
31359753	5	30	theme	biological	1189:1198	arg1	samples					1200:1206	biological samples	1189:1206	biological samples allowed intact lipid A (m/z 1796) to be detected on the bacteria and, despite a 40 keV impact energy, depth	1189:1314	The ability of the GCIB to generate increased higher mass signals from biological samples allowed intact lipid A (m/z 1796) to be detected on the bacteria and, despite a 40 keV impact energy, depth profiled through the bacterial envelope along with other high mass ions including species at m/z 1820 and 2428, attributed to ECACYC, that were only observed below the surface of the bacteria and were notably absent in the depth profile of the lpp mutant.
31359753	3	31	theme	ion	867:869	arg1	signals					871:877	individual ion signals	856:877	individual ion signals	856:877	Analysis of arrays of spotted bacteria allowed changes in the lipid composition of the bacteria to be elucidated using multivariate analysis and confirmed through imaging of individual ion signals.
31359753	5	32	theme	bacterial	1337:1345	arg1	envelope					1347:1354	the bacterial envelope	1333:1354	the bacterial envelope	1333:1354	The ability of the GCIB to generate increased higher mass signals from biological samples allowed intact lipid A (m/z 1796) to be detected on the bacteria and, despite a 40 keV impact energy, depth profiled through the bacterial envelope along with other high mass ions including species at m/z 1820 and 2428, attributed to ECACYC, that were only observed below the surface of the bacteria and were notably absent in the depth profile of the lpp mutant.
31359753	5	33	theme	depth	1539:1543	arg1	profile					1545:1551	the depth profile	1535:1551	the depth profile of the lpp mutant	1535:1569	The ability of the GCIB to generate increased higher mass signals from biological samples allowed intact lipid A (m/z 1796) to be detected on the bacteria and, despite a 40 keV impact energy, depth profiled through the bacterial envelope along with other high mass ions including species at m/z 1820 and 2428, attributed to ECACYC, that were only observed below the surface of the bacteria and were notably absent in the depth profile of the lpp mutant.
31359753	5	34	theme	GCIB	1137:1140	arg1	ability					1122:1128	The ability	1118:1128	The ability of the GCIB to generate increased higher mass signals from biological samples allowed intact lipid A (m/z 1796) to be detected on the bacteria and, despite a 40 keV impact energy, depth	1118:1314	The ability of the GCIB to generate increased higher mass signals from biological samples allowed intact lipid A (m/z 1796) to be detected on the bacteria and, despite a 40 keV impact energy, depth profiled through the bacterial envelope along with other high mass ions including species at m/z 1820 and 2428, attributed to ECACYC, that were only observed below the surface of the bacteria and were notably absent in the depth profile of the lpp mutant.
31359753	2	35	theme	surface	630:636	arg1	useful					674:679	useful	674:679	useful	674:679	The subset of mutants selected were expected to result in changes in the bacterial envelope composition through the deletion of genes encoding for FabF, DapF, and Lpp, where the surface sensitivity of ToF-SIMS can be most useful.
31359753	2	35	theme	surface	630:636	arg1	sensitivity					638:648	the surface sensitivity	626:648	the surface sensitivity of ToF-SIMS	626:660	The subset of mutants selected were expected to result in changes in the bacterial envelope composition through the deletion of genes encoding for FabF, DapF, and Lpp, where the surface sensitivity of ToF-SIMS can be most useful.
31359753	0	36	theme	Chemical	0:7	arg1	Changes					9:15	Chemical Changes	0:15	Chemical Changes On, and Through, The Bacterial Envelope in Escherichia coli Mutants Exhibiting Impaired Plasmid Transfer	0:120	Chemical Changes On, and Through, The Bacterial Envelope in Escherichia coli Mutants Exhibiting Impaired Plasmid Transfer Identified Using Time-of-Flight Secondary Ion Mass Spectrometry.
31359753	1	37	theme	gas	262:264	arg1	GCIB					284:287	GCIB	284:287	GCIB	284:287	Time-of-flight secondary ion mass spectrometry (ToF-SIMS) using a (CO2)6k+ gas cluster ion beam (GCIB) was used to analyze Escherichia coli mutants previously identified as having impaired plasmid transfer capability related to the spread of antibiotic resistance.
31359753	1	37	theme	gas	262:264	arg1	beam					278:281	a (CO2)6k+ gas cluster ion beam	251:281	a (CO2)6k+ gas cluster ion beam (GCIB)	251:288	Time-of-flight secondary ion mass spectrometry (ToF-SIMS) using a (CO2)6k+ gas cluster ion beam (GCIB) was used to analyze Escherichia coli mutants previously identified as having impaired plasmid transfer capability related to the spread of antibiotic resistance.
31359753	4	38	theme	fabF	1005:1008	arg1	mutant					1010:1015	the fabF mutant	1001:1015	the fabF mutant where FabF is associated with the elongation of FA(16:1) to FA(18:1) and not cyclopropane formation	1001:1115	Significant changes in chemical composition were observed, including a surprising loss of cyclopropanated fatty acids in the fabF mutant where FabF is associated with the elongation of FA(16:1) to FA(18:1) and not cyclopropane formation.
31359753	6	39	theme	pathways	1639:1646	arg1	action					1616:1621	the action	1612:1621	the action of the specific pathways targeted in this study	1612:1669	The analysis provides new insights into the action of the specific pathways targeted in this study and paves the way for whole new avenues for the characterization of intact molecules within the bacterial envelope.
31359753	1	40	theme	ion	274:276	arg1	GCIB					284:287	GCIB	284:287	GCIB	284:287	Time-of-flight secondary ion mass spectrometry (ToF-SIMS) using a (CO2)6k+ gas cluster ion beam (GCIB) was used to analyze Escherichia coli mutants previously identified as having impaired plasmid transfer capability related to the spread of antibiotic resistance.
31359753	1	40	theme	ion	274:276	arg1	beam					278:281	a (CO2)6k+ gas cluster ion beam	251:281	a (CO2)6k+ gas cluster ion beam (GCIB)	251:288	Time-of-flight secondary ion mass spectrometry (ToF-SIMS) using a (CO2)6k+ gas cluster ion beam (GCIB) was used to analyze Escherichia coli mutants previously identified as having impaired plasmid transfer capability related to the spread of antibiotic resistance.
31359753	5	41	located	detected	1248:1255	arg1	bacteria					1264:1271	the bacteria	1260:1271	the bacteria	1260:1271	The ability of the GCIB to generate increased higher mass signals from biological samples allowed intact lipid A (m/z 1796) to be detected on the bacteria and, despite a 40 keV impact energy, depth profiled through the bacterial envelope along with other high mass ions including species at m/z 1820 and 2428, attributed to ECACYC, that were only observed below the surface of the bacteria and were notably absent in the depth profile of the lpp mutant.
31359753	5	41	located	detected	1248:1255	arg2	A					1229:1229	intact lipid A	1216:1229	intact lipid A (m/z 1796)	1216:1240	The ability of the GCIB to generate increased higher mass signals from biological samples allowed intact lipid A (m/z 1796) to be detected on the bacteria and, despite a 40 keV impact energy, depth profiled through the bacterial envelope along with other high mass ions including species at m/z 1820 and 2428, attributed to ECACYC, that were only observed below the surface of the bacteria and were notably absent in the depth profile of the lpp mutant.
31359753	5	41	located	detected	1248:1255	arg2	m/z					1232:1234	m/z 1796	1232:1239	m/z 1796	1232:1239	The ability of the GCIB to generate increased higher mass signals from biological samples allowed intact lipid A (m/z 1796) to be detected on the bacteria and, despite a 40 keV impact energy, depth profiled through the bacterial envelope along with other high mass ions including species at m/z 1820 and 2428, attributed to ECACYC, that were only observed below the surface of the bacteria and were notably absent in the depth profile of the lpp mutant.
31359753	4	42	theme	fatty	986:990	arg1	acids					992:996	cyclopropanated fatty acids	970:996	cyclopropanated fatty acids	970:996	Significant changes in chemical composition were observed, including a surprising loss of cyclopropanated fatty acids in the fabF mutant where FabF is associated with the elongation of FA(16:1) to FA(18:1) and not cyclopropane formation.
31359753	5	43	theme	keV	1291:1293	arg1	energy					1302:1307	a 40 keV impact energy	1286:1307	a 40 keV impact energy	1286:1307	The ability of the GCIB to generate increased higher mass signals from biological samples allowed intact lipid A (m/z 1796) to be detected on the bacteria and, despite a 40 keV impact energy, depth profiled through the bacterial envelope along with other high mass ions including species at m/z 1820 and 2428, attributed to ECACYC, that were only observed below the surface of the bacteria and were notably absent in the depth profile of the lpp mutant.
31359753	5	44	theme	40	1288:1289	arg1	keV					1291:1293	keV	1291:1293	keV	1291:1293	The ability of the GCIB to generate increased higher mass signals from biological samples allowed intact lipid A (m/z 1796) to be detected on the bacteria and, despite a 40 keV impact energy, depth profiled through the bacterial envelope along with other high mass ions including species at m/z 1820 and 2428, attributed to ECACYC, that were only observed below the surface of the bacteria and were notably absent in the depth profile of the lpp mutant.
31359753	2	45	from	changes	510:516	arg1	composition					544:554	the bacterial envelope composition	521:554	the bacterial envelope composition	521:554	The subset of mutants selected were expected to result in changes in the bacterial envelope composition through the deletion of genes encoding for FabF, DapF, and Lpp, where the surface sensitivity of ToF-SIMS can be most useful.
31359753	5	46	theme	lpp	1560:1562	arg1	mutant					1564:1569	the lpp mutant	1556:1569	the lpp mutant	1556:1569	The ability of the GCIB to generate increased higher mass signals from biological samples allowed intact lipid A (m/z 1796) to be detected on the bacteria and, despite a 40 keV impact energy, depth profiled through the bacterial envelope along with other high mass ions including species at m/z 1820 and 2428, attributed to ECACYC, that were only observed below the surface of the bacteria and were notably absent in the depth profile of the lpp mutant.
31359753	1	47	dep	Escherichia	310:320	arg1	coli					322:325	coli	322:325	coli	322:325	Time-of-flight secondary ion mass spectrometry (ToF-SIMS) using a (CO2)6k+ gas cluster ion beam (GCIB) was used to analyze Escherichia coli mutants previously identified as having impaired plasmid transfer capability related to the spread of antibiotic resistance.
31359753	4	48	theme	surprising	951:960	arg1	loss					962:965	a surprising loss	949:965	a surprising loss of cyclopropanated fatty acids in the fabF mutant where FabF is associated with the elongation of FA(16:1) to FA(18:1) and not cyclopropane formation	949:1115	Significant changes in chemical composition were observed, including a surprising loss of cyclopropanated fatty acids in the fabF mutant where FabF is associated with the elongation of FA(16:1) to FA(18:1) and not cyclopropane formation.
31359753	2	49	theme	genes	580:584	arg1	deletion					568:575	the deletion	564:575	the deletion of genes encoding for FabF, DapF, and Lpp, where the surface sensitivity of ToF-SIMS can be most useful	564:679	The subset of mutants selected were expected to result in changes in the bacterial envelope composition through the deletion of genes encoding for FabF, DapF, and Lpp, where the surface sensitivity of ToF-SIMS can be most useful.
31359753	1	50	theme	antibiotic	429:438	arg1	resistance					440:449	antibiotic resistance	429:449	antibiotic resistance	429:449	Time-of-flight secondary ion mass spectrometry (ToF-SIMS) using a (CO2)6k+ gas cluster ion beam (GCIB) was used to analyze Escherichia coli mutants previously identified as having impaired plasmid transfer capability related to the spread of antibiotic resistance.
31359753	5	51	theme	impact	1295:1300	arg1	energy					1302:1307	a 40 keV impact energy	1286:1307	a 40 keV impact energy	1286:1307	The ability of the GCIB to generate increased higher mass signals from biological samples allowed intact lipid A (m/z 1796) to be detected on the bacteria and, despite a 40 keV impact energy, depth profiled through the bacterial envelope along with other high mass ions including species at m/z 1820 and 2428, attributed to ECACYC, that were only observed below the surface of the bacteria and were notably absent in the depth profile of the lpp mutant.
31359753	1	52	theme	Time-of-flight	187:200	arg1	ToF-SIMS					235:242	ToF-SIMS	235:242	ToF-SIMS	235:242	Time-of-flight secondary ion mass spectrometry (ToF-SIMS) using a (CO2)6k+ gas cluster ion beam (GCIB) was used to analyze Escherichia coli mutants previously identified as having impaired plasmid transfer capability related to the spread of antibiotic resistance.
31359753	1	52	theme	Time-of-flight	187:200	arg1	spectrometry					221:232	Time-of-flight secondary ion mass spectrometry	187:232	Time-of-flight secondary ion mass spectrometry (ToF-SIMS) using a (CO2)6k+ gas cluster ion beam (GCIB)	187:288	Time-of-flight secondary ion mass spectrometry (ToF-SIMS) using a (CO2)6k+ gas cluster ion beam (GCIB) was used to analyze Escherichia coli mutants previously identified as having impaired plasmid transfer capability related to the spread of antibiotic resistance.
31359753	4	53	theme	FA	1065:1066	arg1	elongation					1051:1060	the elongation	1047:1060	the elongation of FA(16:1) to FA(18:1) and not cyclopropane formation	1047:1115	Significant changes in chemical composition were observed, including a surprising loss of cyclopropanated fatty acids in the fabF mutant where FabF is associated with the elongation of FA(16:1) to FA(18:1) and not cyclopropane formation.
31359753	3	54	theme	spotted	704:710	arg1	bacteria					712:719	spotted bacteria	704:719	spotted bacteria	704:719	Analysis of arrays of spotted bacteria allowed changes in the lipid composition of the bacteria to be elucidated using multivariate analysis and confirmed through imaging of individual ion signals.
31359753	1	55	theme	ion	212:214	arg1	ToF-SIMS					235:242	ToF-SIMS	235:242	ToF-SIMS	235:242	Time-of-flight secondary ion mass spectrometry (ToF-SIMS) using a (CO2)6k+ gas cluster ion beam (GCIB) was used to analyze Escherichia coli mutants previously identified as having impaired plasmid transfer capability related to the spread of antibiotic resistance.
31359753	1	55	theme	ion	212:214	arg1	spectrometry					221:232	Time-of-flight secondary ion mass spectrometry	187:232	Time-of-flight secondary ion mass spectrometry (ToF-SIMS) using a (CO2)6k+ gas cluster ion beam (GCIB)	187:288	Time-of-flight secondary ion mass spectrometry (ToF-SIMS) using a (CO2)6k+ gas cluster ion beam (GCIB) was used to analyze Escherichia coli mutants previously identified as having impaired plasmid transfer capability related to the spread of antibiotic resistance.
31359753	3	56	theme	arrays	694:699	arg1	Analysis					682:689	Analysis	682:689	Analysis of arrays of spotted bacteria	682:719	Analysis of arrays of spotted bacteria allowed changes in the lipid composition of the bacteria to be elucidated using multivariate analysis and confirmed through imaging of individual ion signals.
31359753	4	57	from	changes	892:898	arg1	composition					912:922	chemical composition	903:922	chemical composition	903:922	Significant changes in chemical composition were observed, including a surprising loss of cyclopropanated fatty acids in the fabF mutant where FabF is associated with the elongation of FA(16:1) to FA(18:1) and not cyclopropane formation.
31359753	6	58	theme	whole	1693:1697	arg1	avenues					1703:1709	whole new avenues	1693:1709	whole new avenues for the characterization of intact molecules within the bacterial envelope	1693:1784	The analysis provides new insights into the action of the specific pathways targeted in this study and paves the way for whole new avenues for the characterization of intact molecules within the bacterial envelope.
31359753	0	59	theme	Secondary	154:162	arg1	Spectrometry					173:184	Time-of-Flight Secondary Ion Mass Spectrometry	139:184	Time-of-Flight Secondary Ion Mass Spectrometry	139:184	Chemical Changes On, and Through, The Bacterial Envelope in Escherichia coli Mutants Exhibiting Impaired Plasmid Transfer Identified Using Time-of-Flight Secondary Ion Mass Spectrometry.
31359753	1	60	theme	plasmid	376:382	arg1	capability					393:402	impaired plasmid transfer capability	367:402	impaired plasmid transfer capability related to the spread of antibiotic resistance	367:449	Time-of-flight secondary ion mass spectrometry (ToF-SIMS) using a (CO2)6k+ gas cluster ion beam (GCIB) was used to analyze Escherichia coli mutants previously identified as having impaired plasmid transfer capability related to the spread of antibiotic resistance.
31359753	4	61	theme	Significant	880:890	arg1	changes					892:898	Significant changes	880:898	Significant changes in chemical composition	880:922	Significant changes in chemical composition were observed, including a surprising loss of cyclopropanated fatty acids in the fabF mutant where FabF is associated with the elongation of FA(16:1) to FA(18:1) and not cyclopropane formation.
31359753	2	62	theme	ToF-SIMS	653:660	arg1	useful					674:679	useful	674:679	useful	674:679	The subset of mutants selected were expected to result in changes in the bacterial envelope composition through the deletion of genes encoding for FabF, DapF, and Lpp, where the surface sensitivity of ToF-SIMS can be most useful.
31359753	2	62	theme	ToF-SIMS	653:660	arg1	sensitivity					638:648	the surface sensitivity	626:648	the surface sensitivity of ToF-SIMS	626:660	The subset of mutants selected were expected to result in changes in the bacterial envelope composition through the deletion of genes encoding for FabF, DapF, and Lpp, where the surface sensitivity of ToF-SIMS can be most useful.
31359753	5	63	theme	mass	1378:1381	arg1	species					1398:1404	species	1398:1404	species	1398:1404	The ability of the GCIB to generate increased higher mass signals from biological samples allowed intact lipid A (m/z 1796) to be detected on the bacteria and, despite a 40 keV impact energy, depth profiled through the bacterial envelope along with other high mass ions including species at m/z 1820 and 2428, attributed to ECACYC, that were only observed below the surface of the bacteria and were notably absent in the depth profile of the lpp mutant.
31359753	5	63	theme	mass	1378:1381	arg1	ions					1383:1386	other high mass ions	1367:1386	other high mass ions including species at m/z 1820 and 2428, attributed to ECACYC, that were only observed below the surface of the bacteria and were notably absent in the depth profile of the lpp mutant	1367:1569	The ability of the GCIB to generate increased higher mass signals from biological samples allowed intact lipid A (m/z 1796) to be detected on the bacteria and, despite a 40 keV impact energy, depth profiled through the bacterial envelope along with other high mass ions including species at m/z 1820 and 2428, attributed to ECACYC, that were only observed below the surface of the bacteria and were notably absent in the depth profile of the lpp mutant.
31359753	0	64	theme	Mass	168:171	arg1	Spectrometry					173:184	Time-of-Flight Secondary Ion Mass Spectrometry	139:184	Time-of-Flight Secondary Ion Mass Spectrometry	139:184	Chemical Changes On, and Through, The Bacterial Envelope in Escherichia coli Mutants Exhibiting Impaired Plasmid Transfer Identified Using Time-of-Flight Secondary Ion Mass Spectrometry.
31359753	2	65	theme	envelope	535:542	arg1	composition					544:554	the bacterial envelope composition	521:554	the bacterial envelope composition	521:554	The subset of mutants selected were expected to result in changes in the bacterial envelope composition through the deletion of genes encoding for FabF, DapF, and Lpp, where the surface sensitivity of ToF-SIMS can be most useful.
31359753	5	66	dep	ability	1122:1128	arg1	generate					1145:1152	generate	1145:1152	to generate increased higher mass signals from biological samples allowed intact lipid A (m/z 1796) to be detected on the bacteria and, despite a 40 keV impact energy, depth	1142:1314	The ability of the GCIB to generate increased higher mass signals from biological samples allowed intact lipid A (m/z 1796) to be detected on the bacteria and, despite a 40 keV impact energy, depth profiled through the bacterial envelope along with other high mass ions including species at m/z 1820 and 2428, attributed to ECACYC, that were only observed below the surface of the bacteria and were notably absent in the depth profile of the lpp mutant.
31359753	5	67	theme	intact	1216:1221	arg1	m/z					1232:1234	m/z 1796	1232:1239	m/z 1796	1232:1239	The ability of the GCIB to generate increased higher mass signals from biological samples allowed intact lipid A (m/z 1796) to be detected on the bacteria and, despite a 40 keV impact energy, depth profiled through the bacterial envelope along with other high mass ions including species at m/z 1820 and 2428, attributed to ECACYC, that were only observed below the surface of the bacteria and were notably absent in the depth profile of the lpp mutant.
31359753	5	67	theme	intact	1216:1221	arg1	A					1229:1229	intact lipid A	1216:1229	intact lipid A (m/z 1796)	1216:1240	The ability of the GCIB to generate increased higher mass signals from biological samples allowed intact lipid A (m/z 1796) to be detected on the bacteria and, despite a 40 keV impact energy, depth profiled through the bacterial envelope along with other high mass ions including species at m/z 1820 and 2428, attributed to ECACYC, that were only observed below the surface of the bacteria and were notably absent in the depth profile of the lpp mutant.
31359753	5	68	theme	mass	1171:1174	arg1	signals					1176:1182	higher mass signals	1164:1182	higher mass signals from biological samples allowed intact lipid A (m/z 1796) to be detected on the bacteria and, despite a 40 keV impact energy, depth	1164:1314	The ability of the GCIB to generate increased higher mass signals from biological samples allowed intact lipid A (m/z 1796) to be detected on the bacteria and, despite a 40 keV impact energy, depth profiled through the bacterial envelope along with other high mass ions including species at m/z 1820 and 2428, attributed to ECACYC, that were only observed below the surface of the bacteria and were notably absent in the depth profile of the lpp mutant.
31359753	3	69	theme	lipid	744:748	arg1	composition					750:760	the lipid composition	740:760	the lipid composition of the bacteria to be elucidated using multivariate analysis and confirmed through imaging of individual ion signals	740:877	Analysis of arrays of spotted bacteria allowed changes in the lipid composition of the bacteria to be elucidated using multivariate analysis and confirmed through imaging of individual ion signals.
31359753	0	70	theme	Escherichia	60:70	arg1	Mutants					77:83	Escherichia coli Mutants	60:83	Escherichia coli Mutants Exhibiting Impaired Plasmid Transfer	60:120	Chemical Changes On, and Through, The Bacterial Envelope in Escherichia coli Mutants Exhibiting Impaired Plasmid Transfer Identified Using Time-of-Flight Secondary Ion Mass Spectrometry.
31359753	5	71	from	2428	1422:1425	arg1	species					1398:1404	species	1398:1404	species	1398:1404	The ability of the GCIB to generate increased higher mass signals from biological samples allowed intact lipid A (m/z 1796) to be detected on the bacteria and, despite a 40 keV impact energy, depth profiled through the bacterial envelope along with other high mass ions including species at m/z 1820 and 2428, attributed to ECACYC, that were only observed below the surface of the bacteria and were notably absent in the depth profile of the lpp mutant.
31359753	0	72	from	Changes	9:15	arg1	Mutants					77:83	Escherichia coli Mutants	60:83	Escherichia coli Mutants Exhibiting Impaired Plasmid Transfer	60:120	Chemical Changes On, and Through, The Bacterial Envelope in Escherichia coli Mutants Exhibiting Impaired Plasmid Transfer Identified Using Time-of-Flight Secondary Ion Mass Spectrometry.
31359753	5	73	theme	high	1373:1376	arg1	species					1398:1404	species	1398:1404	species	1398:1404	The ability of the GCIB to generate increased higher mass signals from biological samples allowed intact lipid A (m/z 1796) to be detected on the bacteria and, despite a 40 keV impact energy, depth profiled through the bacterial envelope along with other high mass ions including species at m/z 1820 and 2428, attributed to ECACYC, that were only observed below the surface of the bacteria and were notably absent in the depth profile of the lpp mutant.
31359753	5	73	theme	high	1373:1376	arg1	ions					1383:1386	other high mass ions	1367:1386	other high mass ions including species at m/z 1820 and 2428, attributed to ECACYC, that were only observed below the surface of the bacteria and were notably absent in the depth profile of the lpp mutant	1367:1569	The ability of the GCIB to generate increased higher mass signals from biological samples allowed intact lipid A (m/z 1796) to be detected on the bacteria and, despite a 40 keV impact energy, depth profiled through the bacterial envelope along with other high mass ions including species at m/z 1820 and 2428, attributed to ECACYC, that were only observed below the surface of the bacteria and were notably absent in the depth profile of the lpp mutant.
31359753	6	74	theme	molecules	1746:1754	arg1	characterization					1719:1734	the characterization	1715:1734	the characterization of intact molecules within the bacterial envelope	1715:1784	The analysis provides new insights into the action of the specific pathways targeted in this study and paves the way for whole new avenues for the characterization of intact molecules within the bacterial envelope.
31359753	1	75	theme	Escherichia	310:320	arg1	mutants					327:333	Escherichia coli mutants	310:333	Escherichia coli mutants previously identified as having impaired plasmid transfer capability related to the spread of antibiotic resistance	310:449	Time-of-flight secondary ion mass spectrometry (ToF-SIMS) using a (CO2)6k+ gas cluster ion beam (GCIB) was used to analyze Escherichia coli mutants previously identified as having impaired plasmid transfer capability related to the spread of antibiotic resistance.
31359753	4	76	theme	chemical	903:910	arg1	composition					912:922	chemical composition	903:922	chemical composition	903:922	Significant changes in chemical composition were observed, including a surprising loss of cyclopropanated fatty acids in the fabF mutant where FabF is associated with the elongation of FA(16:1) to FA(18:1) and not cyclopropane formation.
31359753	3	77	theme	bacteria	769:776	arg1	composition					750:760	the lipid composition	740:760	the lipid composition of the bacteria to be elucidated using multivariate analysis and confirmed through imaging of individual ion signals	740:877	Analysis of arrays of spotted bacteria allowed changes in the lipid composition of the bacteria to be elucidated using multivariate analysis and confirmed through imaging of individual ion signals.
31359753	0	78	theme	Impaired	96:103	arg1	Transfer					113:120	Impaired Plasmid Transfer	96:120	Impaired Plasmid Transfer	96:120	Chemical Changes On, and Through, The Bacterial Envelope in Escherichia coli Mutants Exhibiting Impaired Plasmid Transfer Identified Using Time-of-Flight Secondary Ion Mass Spectrometry.
31359753	3	79	theme	signals	871:877	arg1	imaging					845:851	imaging	845:851	imaging of individual ion signals	845:877	Analysis of arrays of spotted bacteria allowed changes in the lipid composition of the bacteria to be elucidated using multivariate analysis and confirmed through imaging of individual ion signals.
31359753	0	80	dep	Escherichia	60:70	arg1	coli					72:75	coli	72:75	coli	72:75	Chemical Changes On, and Through, The Bacterial Envelope in Escherichia coli Mutants Exhibiting Impaired Plasmid Transfer Identified Using Time-of-Flight Secondary Ion Mass Spectrometry.
31359753	3	81	theme	individual	856:865	arg1	signals					871:877	individual ion signals	856:877	individual ion signals	856:877	Analysis of arrays of spotted bacteria allowed changes in the lipid composition of the bacteria to be elucidated using multivariate analysis and confirmed through imaging of individual ion signals.
31359753	4	82	theme	acids	992:996	arg1	loss					962:965	a surprising loss	949:965	a surprising loss of cyclopropanated fatty acids in the fabF mutant where FabF is associated with the elongation of FA(16:1) to FA(18:1) and not cyclopropane formation	949:1115	Significant changes in chemical composition were observed, including a surprising loss of cyclopropanated fatty acids in the fabF mutant where FabF is associated with the elongation of FA(16:1) to FA(18:1) and not cyclopropane formation.
31359753	1	83	theme	cluster	266:272	arg1	GCIB					284:287	GCIB	284:287	GCIB	284:287	Time-of-flight secondary ion mass spectrometry (ToF-SIMS) using a (CO2)6k+ gas cluster ion beam (GCIB) was used to analyze Escherichia coli mutants previously identified as having impaired plasmid transfer capability related to the spread of antibiotic resistance.
31359753	1	83	theme	cluster	266:272	arg1	beam					278:281	a (CO2)6k+ gas cluster ion beam	251:281	a (CO2)6k+ gas cluster ion beam (GCIB)	251:288	Time-of-flight secondary ion mass spectrometry (ToF-SIMS) using a (CO2)6k+ gas cluster ion beam (GCIB) was used to analyze Escherichia coli mutants previously identified as having impaired plasmid transfer capability related to the spread of antibiotic resistance.
31359753	6	84	theme	specific	1630:1637	arg1	pathways					1639:1646	the specific pathways	1626:1646	the specific pathways targeted in this study	1626:1669	The analysis provides new insights into the action of the specific pathways targeted in this study and paves the way for whole new avenues for the characterization of intact molecules within the bacterial envelope.
31359753	4	85	theme	cyclopropanated	970:984	arg1	acids					992:996	cyclopropanated fatty acids	970:996	cyclopropanated fatty acids	970:996	Significant changes in chemical composition were observed, including a surprising loss of cyclopropanated fatty acids in the fabF mutant where FabF is associated with the elongation of FA(16:1) to FA(18:1) and not cyclopropane formation.
31359753	4	86	from	loss	962:965	arg1	mutant					1010:1015	the fabF mutant	1001:1015	the fabF mutant where FabF is associated with the elongation of FA(16:1) to FA(18:1) and not cyclopropane formation	1001:1115	Significant changes in chemical composition were observed, including a surprising loss of cyclopropanated fatty acids in the fabF mutant where FabF is associated with the elongation of FA(16:1) to FA(18:1) and not cyclopropane formation.
31359753	5	87	from	absent	1525:1530	arg1	profile					1545:1551	the depth profile	1535:1551	the depth profile of the lpp mutant	1535:1569	The ability of the GCIB to generate increased higher mass signals from biological samples allowed intact lipid A (m/z 1796) to be detected on the bacteria and, despite a 40 keV impact energy, depth profiled through the bacterial envelope along with other high mass ions including species at m/z 1820 and 2428, attributed to ECACYC, that were only observed below the surface of the bacteria and were notably absent in the depth profile of the lpp mutant.
31359753	5	88	theme	mutant	1564:1569	arg1	profile					1545:1551	the depth profile	1535:1551	the depth profile of the lpp mutant	1535:1569	The ability of the GCIB to generate increased higher mass signals from biological samples allowed intact lipid A (m/z 1796) to be detected on the bacteria and, despite a 40 keV impact energy, depth profiled through the bacterial envelope along with other high mass ions including species at m/z 1820 and 2428, attributed to ECACYC, that were only observed below the surface of the bacteria and were notably absent in the depth profile of the lpp mutant.
31359753	5	89	theme	higher	1164:1169	arg1	signals					1176:1182	higher mass signals	1164:1182	higher mass signals from biological samples allowed intact lipid A (m/z 1796) to be detected on the bacteria and, despite a 40 keV impact energy, depth	1164:1314	The ability of the GCIB to generate increased higher mass signals from biological samples allowed intact lipid A (m/z 1796) to be detected on the bacteria and, despite a 40 keV impact energy, depth profiled through the bacterial envelope along with other high mass ions including species at m/z 1820 and 2428, attributed to ECACYC, that were only observed below the surface of the bacteria and were notably absent in the depth profile of the lpp mutant.
31096241	6	0	theme	major	919:923	arg1	mortality					978:986	major and clinically relevant nonmajor bleeding, all-cause mortality	919:986	mortality	978:986	Secondary endpoints included the incidence of recurrence, major and clinically relevant nonmajor bleeding, all-cause mortality, and bleeding or pulmonary embolism-related mortality.
31096241	9	1	theme	dalteparin	1600:1609	arg1	group					1611:1615	the dalteparin group	1596:1615	the dalteparin group	1596:1615	There was no statistically significant intergroup difference for the incidence of VTE recurrence (5.3% in the rivaroxaban group versus 2.7% in the dalteparin group, p = 0.495) and major or clinically relevant nonmajor bleeding (23.7% in the rivaroxaban group versus 13.7% in the dalteparin group, p = 0.089).
31096241	10	2	theme	all-cause	1805:1813	arg1	rate					1825:1828	the all-cause mortality rate	1801:1828	the all-cause mortality rate (hazard ratio 0.864, 95% CI 0.624-1.196, p = 0.337)	1801:1880	There was no significant difference in the all-cause mortality rate (hazard ratio 0.864, 95% CI 0.624-1.196, p = 0.337).
31096241	3	3	from	VTE	530:532	arg1	patients					537:544	patients	537:544	patients with primary lung cancer	537:569	OBJECTIVES We examined the efficacy and safety of rivaroxaban compared with dalteparin for cancer-associated VTE in patients with primary lung cancer.
31096241	6	4	theme	recurrence	907:916	arg1	incidence					894:902	the incidence	890:902	the incidence of recurrence, major and clinically relevant nonmajor bleeding, all-cause mortality, and bleeding or pulmonary embolism-related mortality	890:1040	Secondary endpoints included the incidence of recurrence, major and clinically relevant nonmajor bleeding, all-cause mortality, and bleeding or pulmonary embolism-related mortality.
31096241	4	5	theme	patients	623:630	arg1	study					610:614	A single-center retrospective study	580:614	METHODS A single-center retrospective study of 204 patients with primary lung cancer who were prescribed rivaroxaban (n = 131) or dalteparin (n = 73) for VTE	572:728	METHODS A single-center retrospective study of 204 patients with primary lung cancer who were prescribed rivaroxaban (n = 131) or dalteparin (n = 73) for VTE was performed.
31096241	2	6	theme	anticoagulants	290:303	arg1	use					271:273	The use	267:273	The use of direct oral anticoagulants for cancer-associated VTE	267:329	The use of direct oral anticoagulants for cancer-associated VTE has increased; however, their efficacy and safety in lung cancer patients remain unclear.
31096241	4	7	theme	=	716:716	arg1	dalteparin					702:711	dalteparin	702:711	dalteparin (n = 73)	702:720	METHODS A single-center retrospective study of 204 patients with primary lung cancer who were prescribed rivaroxaban (n = 131) or dalteparin (n = 73) for VTE was performed.
31096241	4	7	theme	=	716:716	arg1	n					714:714	n = 73	714:719	n = 73	714:719	METHODS A single-center retrospective study of 204 patients with primary lung cancer who were prescribed rivaroxaban (n = 131) or dalteparin (n = 73) for VTE was performed.
31096241	8	8	theme	proportional	1206:1217	arg1	model					1227:1231	The multivariate Cox proportional hazards model	1185:1231	The multivariate Cox proportional hazards model for age, Eastern Cooperative Oncology Group performance score, and bleeding risk factors	1185:1320	The multivariate Cox proportional hazards model for age, Eastern Cooperative Oncology Group performance score, and bleeding risk factors revealed the rivaroxaban group showed a 1.176-fold composite event risk without statistical significance (0.595-2.324, p = 0.641).
31096241	10	9	theme	hazard	1831:1836	arg1	ratio					1838:1842	hazard ratio 0.864	1831:1848	hazard ratio 0.864	1831:1848	There was no significant difference in the all-cause mortality rate (hazard ratio 0.864, 95% CI 0.624-1.196, p = 0.337).
31096241	2	10	theme	direct	278:283	arg1	anticoagulants					290:303	direct oral anticoagulants	278:303	direct oral anticoagulants	278:303	The use of direct oral anticoagulants for cancer-associated VTE has increased; however, their efficacy and safety in lung cancer patients remain unclear.
31096241	2	11	from	efficacy	361:368	arg1	patients					396:403	lung cancer patients	384:403	lung cancer patients	384:403	The use of direct oral anticoagulants for cancer-associated VTE has increased; however, their efficacy and safety in lung cancer patients remain unclear.
31096241	11	12	theme	rivaroxaban	1957:1967	arg1	profile					1946:1952	the safety and efficacy profile	1922:1952	the safety and efficacy profile of rivaroxaban	1922:1967	CONCLUSIONS There was no difference in the safety and efficacy profile of rivaroxaban compared with dalteparin.
31096241	1	13	theme	cancer-associated	187:203	arg1	thromboembolism					212:226	cancer-associated venous thromboembolism	187:226	cancer-associated venous thromboembolism (VTE)	187:232	BACKGROUND Standard therapy for cancer-associated venous thromboembolism (VTE) is low-molecular-weight heparin.
31096241	1	13	theme	cancer-associated	187:203	arg1	VTE					229:231	VTE	229:231	VTE	229:231	BACKGROUND Standard therapy for cancer-associated venous thromboembolism (VTE) is low-molecular-weight heparin.
31096241	6	14	theme	mortality	978:986	arg1	incidence					894:902	the incidence	890:902	the incidence of recurrence, major and clinically relevant nonmajor bleeding, all-cause mortality, and bleeding or pulmonary embolism-related mortality	890:1040	Secondary endpoints included the incidence of recurrence, major and clinically relevant nonmajor bleeding, all-cause mortality, and bleeding or pulmonary embolism-related mortality.
31096241	9	15	theme	major	1633:1637	arg1	incidence					1522:1530	the incidence	1518:1530	the incidence of VTE recurrence (5.3% in the rivaroxaban group versus 2.7% in the dalteparin group, p = 0.495) and major or clinically relevant nonmajor bleeding (23.7% in the rivaroxaban group versus 13.7% in the dalteparin group, p = 0.089)	1518:1759	There was no statistically significant intergroup difference for the incidence of VTE recurrence (5.3% in the rivaroxaban group versus 2.7% in the dalteparin group, p = 0.495) and major or clinically relevant nonmajor bleeding (23.7% in the rivaroxaban group versus 13.7% in the dalteparin group, p = 0.089).
31096241	2	16	from	safety	374:379	arg1	patients					396:403	lung cancer patients	384:403	lung cancer patients	384:403	The use of direct oral anticoagulants for cancer-associated VTE has increased; however, their efficacy and safety in lung cancer patients remain unclear.
31096241	0	17	theme	Thromboembolism	109:123	arg1	Treatment					89:97	Long-Term Treatment	79:97	Long-Term Treatment of Venous Thromboembolism in Patients with Lung Cancer	79:152	A Retrospective Study on Efficacy and Safety of Rivaroxaban and Dalteparin for Long-Term Treatment of Venous Thromboembolism in Patients with Lung Cancer.
31096241	2	18	theme	cancer-associated	309:325	arg1	VTE					327:329	cancer-associated VTE	309:329	cancer-associated VTE	309:329	The use of direct oral anticoagulants for cancer-associated VTE has increased; however, their efficacy and safety in lung cancer patients remain unclear.
31096241	8	19	dep	revealed	1322:1329	arg1	showed					1353:1358	showed	1353:1358	revealed the rivaroxaban group showed a 1.176-fold composite event risk without statistical significance (0.595-2.324, p = 0.641)	1322:1450	The multivariate Cox proportional hazards model for age, Eastern Cooperative Oncology Group performance score, and bleeding risk factors revealed the rivaroxaban group showed a 1.176-fold composite event risk without statistical significance (0.595-2.324, p = 0.641).
31096241	9	20	dep	recurrence	1539:1548	arg1	=					1620:1620	=	1620:1620	=	1620:1620	There was no statistically significant intergroup difference for the incidence of VTE recurrence (5.3% in the rivaroxaban group versus 2.7% in the dalteparin group, p = 0.495) and major or clinically relevant nonmajor bleeding (23.7% in the rivaroxaban group versus 13.7% in the dalteparin group, p = 0.089).
31096241	8	21	theme	Eastern	1242:1248	arg1	score					1289:1293	Eastern Cooperative Oncology Group performance score	1242:1293	Eastern Cooperative Oncology Group performance score	1242:1293	The multivariate Cox proportional hazards model for age, Eastern Cooperative Oncology Group performance score, and bleeding risk factors revealed the rivaroxaban group showed a 1.176-fold composite event risk without statistical significance (0.595-2.324, p = 0.641).
31096241	7	22	theme	38	1083:1084	arg1	patients					1108:1115	38 (29.0) and 12 (16.4%) patients	1083:1115	38 (29.0) and 12 (16.4%) patients in the rivaroxaban and dalteparin (p = 0.045) groups	1083:1168	RESULTS The composite event occurred in 38 (29.0) and 12 (16.4%) patients in the rivaroxaban and dalteparin (p = 0.045) groups, respectively.
31096241	8	23	theme	multivariate	1189:1200	arg1	model					1227:1231	The multivariate Cox proportional hazards model	1185:1231	The multivariate Cox proportional hazards model for age, Eastern Cooperative Oncology Group performance score, and bleeding risk factors	1185:1320	The multivariate Cox proportional hazards model for age, Eastern Cooperative Oncology Group performance score, and bleeding risk factors revealed the rivaroxaban group showed a 1.176-fold composite event risk without statistical significance (0.595-2.324, p = 0.641).
31096241	6	24	theme	nonmajor	949:956	arg1	mortality					978:986	major and clinically relevant nonmajor bleeding, all-cause mortality	919:986	mortality	978:986	Secondary endpoints included the incidence of recurrence, major and clinically relevant nonmajor bleeding, all-cause mortality, and bleeding or pulmonary embolism-related mortality.
31096241	8	25	theme	Oncology	1262:1269	arg1	score					1289:1293	Eastern Cooperative Oncology Group performance score	1242:1293	Eastern Cooperative Oncology Group performance score	1242:1293	The multivariate Cox proportional hazards model for age, Eastern Cooperative Oncology Group performance score, and bleeding risk factors revealed the rivaroxaban group showed a 1.176-fold composite event risk without statistical significance (0.595-2.324, p = 0.641).
31096241	0	26	theme	Lung	142:145	arg1	Cancer					147:152	Lung Cancer	142:152	Lung Cancer	142:152	A Retrospective Study on Efficacy and Safety of Rivaroxaban and Dalteparin for Long-Term Treatment of Venous Thromboembolism in Patients with Lung Cancer.
31096241	9	27	from	%	1554:1554	arg1	group					1575:1579	the rivaroxaban group	1559:1579	the rivaroxaban group	1559:1579	There was no statistically significant intergroup difference for the incidence of VTE recurrence (5.3% in the rivaroxaban group versus 2.7% in the dalteparin group, p = 0.495) and major or clinically relevant nonmajor bleeding (23.7% in the rivaroxaban group versus 13.7% in the dalteparin group, p = 0.089).
31096241	11	28	theme	efficacy	1937:1944	arg1	profile					1946:1952	the safety and efficacy profile	1922:1952	the safety and efficacy profile of rivaroxaban	1922:1967	CONCLUSIONS There was no difference in the safety and efficacy profile of rivaroxaban compared with dalteparin.
31096241	8	29	theme	performance	1277:1287	arg1	score					1289:1293	Eastern Cooperative Oncology Group performance score	1242:1293	Eastern Cooperative Oncology Group performance score	1242:1293	The multivariate Cox proportional hazards model for age, Eastern Cooperative Oncology Group performance score, and bleeding risk factors revealed the rivaroxaban group showed a 1.176-fold composite event risk without statistical significance (0.595-2.324, p = 0.641).
31096241	0	30	with	Patients	128:135	arg1	Cancer					147:152	Lung Cancer	142:152	Lung Cancer	142:152	A Retrospective Study on Efficacy and Safety of Rivaroxaban and Dalteparin for Long-Term Treatment of Venous Thromboembolism in Patients with Lung Cancer.
31096241	7	31	theme	composite	1055:1063	arg1	event					1065:1069	The composite event	1051:1069	The composite event	1051:1069	RESULTS The composite event occurred in 38 (29.0) and 12 (16.4%) patients in the rivaroxaban and dalteparin (p = 0.045) groups, respectively.
31096241	9	32	theme	significant	1480:1490	arg1	difference					1503:1512	no statistically significant intergroup difference	1463:1512	no statistically significant intergroup difference	1463:1512	There was no statistically significant intergroup difference for the incidence of VTE recurrence (5.3% in the rivaroxaban group versus 2.7% in the dalteparin group, p = 0.495) and major or clinically relevant nonmajor bleeding (23.7% in the rivaroxaban group versus 13.7% in the dalteparin group, p = 0.089).
31096241	0	33	theme	Rivaroxaban	48:58	arg1	Safety					38:43	Safety	38:43	Safety	38:43	A Retrospective Study on Efficacy and Safety of Rivaroxaban and Dalteparin for Long-Term Treatment of Venous Thromboembolism in Patients with Lung Cancer.
31096241	0	33	theme	Rivaroxaban	48:58	arg1	Efficacy					25:32	Efficacy	25:32	Efficacy	25:32	A Retrospective Study on Efficacy and Safety of Rivaroxaban and Dalteparin for Long-Term Treatment of Venous Thromboembolism in Patients with Lung Cancer.
31096241	6	34	theme	embolism-related	1015:1030	arg1	mortality					1032:1040	bleeding or pulmonary embolism-related mortality	993:1040	bleeding or pulmonary embolism-related mortality	993:1040	Secondary endpoints included the incidence of recurrence, major and clinically relevant nonmajor bleeding, all-cause mortality, and bleeding or pulmonary embolism-related mortality.
31096241	0	35	from	Study	16:20	arg1	Safety					38:43	Safety	38:43	Safety	38:43	A Retrospective Study on Efficacy and Safety of Rivaroxaban and Dalteparin for Long-Term Treatment of Venous Thromboembolism in Patients with Lung Cancer.
31096241	0	35	from	Study	16:20	arg1	Efficacy					25:32	Efficacy	25:32	Efficacy	25:32	A Retrospective Study on Efficacy and Safety of Rivaroxaban and Dalteparin for Long-Term Treatment of Venous Thromboembolism in Patients with Lung Cancer.
31096241	8	36	theme	bleeding	1300:1307	arg1	factors					1314:1320	bleeding risk factors	1300:1320	bleeding risk factors	1300:1320	The multivariate Cox proportional hazards model for age, Eastern Cooperative Oncology Group performance score, and bleeding risk factors revealed the rivaroxaban group showed a 1.176-fold composite event risk without statistical significance (0.595-2.324, p = 0.641).
31096241	0	37	theme	Dalteparin	64:73	arg1	Safety					38:43	Safety	38:43	Safety	38:43	A Retrospective Study on Efficacy and Safety of Rivaroxaban and Dalteparin for Long-Term Treatment of Venous Thromboembolism in Patients with Lung Cancer.
31096241	0	37	theme	Dalteparin	64:73	arg1	Efficacy					25:32	Efficacy	25:32	Efficacy	25:32	A Retrospective Study on Efficacy and Safety of Rivaroxaban and Dalteparin for Long-Term Treatment of Venous Thromboembolism in Patients with Lung Cancer.
31096241	1	38	theme	BACKGROUND	155:164	arg1	therapy					175:181	BACKGROUND Standard therapy	155:181	BACKGROUND Standard therapy for cancer-associated venous thromboembolism (VTE)	155:232	BACKGROUND Standard therapy for cancer-associated venous thromboembolism (VTE) is low-molecular-weight heparin.
31096241	0	39	theme	Long-Term	79:87	arg1	Treatment					89:97	Long-Term Treatment	79:97	Long-Term Treatment of Venous Thromboembolism in Patients with Lung Cancer	79:152	A Retrospective Study on Efficacy and Safety of Rivaroxaban and Dalteparin for Long-Term Treatment of Venous Thromboembolism in Patients with Lung Cancer.
31096241	11	40	dep	CONCLUSIONS	1883:1893	arg1	was					1901:1903	was	1901:1903	was no difference in the safety and efficacy profile of rivaroxaban compared with dalteparin	1901:1992	CONCLUSIONS There was no difference in the safety and efficacy profile of rivaroxaban compared with dalteparin.
31096241	9	41	dep	bleeding	1671:1678	arg1	=					1752:1752	=	1752:1752	=	1752:1752	There was no statistically significant intergroup difference for the incidence of VTE recurrence (5.3% in the rivaroxaban group versus 2.7% in the dalteparin group, p = 0.495) and major or clinically relevant nonmajor bleeding (23.7% in the rivaroxaban group versus 13.7% in the dalteparin group, p = 0.089).
31096241	9	42	theme	recurrence	1539:1548	arg1	incidence					1522:1530	the incidence	1518:1530	the incidence of VTE recurrence (5.3% in the rivaroxaban group versus 2.7% in the dalteparin group, p = 0.495) and major or clinically relevant nonmajor bleeding (23.7% in the rivaroxaban group versus 13.7% in the dalteparin group, p = 0.089)	1518:1759	There was no statistically significant intergroup difference for the incidence of VTE recurrence (5.3% in the rivaroxaban group versus 2.7% in the dalteparin group, p = 0.495) and major or clinically relevant nonmajor bleeding (23.7% in the rivaroxaban group versus 13.7% in the dalteparin group, p = 0.089).
31096241	3	43	dep	OBJECTIVES	421:430	arg1	examined					435:442	examined	435:442	examined the efficacy and safety of rivaroxaban compared with dalteparin for cancer-associated VTE in patients with primary lung cancer	435:569	OBJECTIVES We examined the efficacy and safety of rivaroxaban compared with dalteparin for cancer-associated VTE in patients with primary lung cancer.
31096241	7	44	dep	RESULTS	1043:1049	arg1	occurred					1071:1078	occurred	1071:1078	occurred	1071:1078	RESULTS The composite event occurred in 38 (29.0) and 12 (16.4%) patients in the rivaroxaban and dalteparin (p = 0.045) groups, respectively.
31096241	2	45	theme	cancer	389:394	arg1	patients					396:403	lung cancer patients	384:403	lung cancer patients	384:403	The use of direct oral anticoagulants for cancer-associated VTE has increased; however, their efficacy and safety in lung cancer patients remain unclear.
31096241	7	46	theme	=	1154:1154	arg1	rivaroxaban					1124:1134	rivaroxaban	1124:1134	rivaroxaban	1124:1134	RESULTS The composite event occurred in 38 (29.0) and 12 (16.4%) patients in the rivaroxaban and dalteparin (p = 0.045) groups, respectively.
31096241	7	46	theme	=	1154:1154	arg1	0.045					1156:1160	p = 0.045	1152:1160	p = 0.045	1152:1160	RESULTS The composite event occurred in 38 (29.0) and 12 (16.4%) patients in the rivaroxaban and dalteparin (p = 0.045) groups, respectively.
31096241	9	47	dep	=	1752:1752	arg1	%					1685:1685	23.7%	1681:1685	23.7% in the rivaroxaban group	1681:1710	There was no statistically significant intergroup difference for the incidence of VTE recurrence (5.3% in the rivaroxaban group versus 2.7% in the dalteparin group, p = 0.495) and major or clinically relevant nonmajor bleeding (23.7% in the rivaroxaban group versus 13.7% in the dalteparin group, p = 0.089).
31096241	9	47	dep	=	1752:1752	arg1	%					1723:1723	13.7%	1719:1723	13.7% in the dalteparin group	1719:1747	There was no statistically significant intergroup difference for the incidence of VTE recurrence (5.3% in the rivaroxaban group versus 2.7% in the dalteparin group, p = 0.495) and major or clinically relevant nonmajor bleeding (23.7% in the rivaroxaban group versus 13.7% in the dalteparin group, p = 0.089).
31096241	9	48	from	%	1723:1723	arg1	group					1743:1747	the dalteparin group	1728:1747	the dalteparin group	1728:1747	There was no statistically significant intergroup difference for the incidence of VTE recurrence (5.3% in the rivaroxaban group versus 2.7% in the dalteparin group, p = 0.495) and major or clinically relevant nonmajor bleeding (23.7% in the rivaroxaban group versus 13.7% in the dalteparin group, p = 0.089).
31096241	9	49	theme	dalteparin	1732:1741	arg1	group					1743:1747	the dalteparin group	1728:1747	the dalteparin group	1728:1747	There was no statistically significant intergroup difference for the incidence of VTE recurrence (5.3% in the rivaroxaban group versus 2.7% in the dalteparin group, p = 0.495) and major or clinically relevant nonmajor bleeding (23.7% in the rivaroxaban group versus 13.7% in the dalteparin group, p = 0.089).
31096241	3	50	theme	rivaroxaban	471:481	arg1	efficacy					448:455	efficacy	448:455	efficacy	448:455	OBJECTIVES We examined the efficacy and safety of rivaroxaban compared with dalteparin for cancer-associated VTE in patients with primary lung cancer.
31096241	3	50	theme	rivaroxaban	471:481	arg1	safety					461:466	safety	461:466	safety	461:466	OBJECTIVES We examined the efficacy and safety of rivaroxaban compared with dalteparin for cancer-associated VTE in patients with primary lung cancer.
31096241	5	51	theme	major	813:817	arg1	nonmajor					842:849	major or clinically relevant nonmajor bleeding	813:858	major or clinically relevant nonmajor bleeding	813:858	The primary endpoint was a composite event including recurrence and major or clinically relevant nonmajor bleeding.
31096241	3	52	theme	primary	551:557	arg1	cancer					564:569	primary lung cancer	551:569	primary lung cancer	551:569	OBJECTIVES We examined the efficacy and safety of rivaroxaban compared with dalteparin for cancer-associated VTE in patients with primary lung cancer.
31096241	4	53	theme	single-center	582:594	arg1	study					610:614	A single-center retrospective study	580:614	METHODS A single-center retrospective study of 204 patients with primary lung cancer who were prescribed rivaroxaban (n = 131) or dalteparin (n = 73) for VTE	572:728	METHODS A single-center retrospective study of 204 patients with primary lung cancer who were prescribed rivaroxaban (n = 131) or dalteparin (n = 73) for VTE was performed.
31096241	8	54	theme	composite	1373:1381	arg1	event					1383:1387	a 1.176-fold composite event	1360:1387	a 1.176-fold composite event risk	1360:1392	The multivariate Cox proportional hazards model for age, Eastern Cooperative Oncology Group performance score, and bleeding risk factors revealed the rivaroxaban group showed a 1.176-fold composite event risk without statistical significance (0.595-2.324, p = 0.641).
31096241	5	55	theme	bleeding	851:858	arg1	nonmajor					842:849	major or clinically relevant nonmajor bleeding	813:858	major or clinically relevant nonmajor bleeding	813:858	The primary endpoint was a composite event including recurrence and major or clinically relevant nonmajor bleeding.
31096241	8	56	dep	=	1443:1443	arg1	0.595-2.324					1428:1438	0.595-2.324	1428:1438	0.595-2.324	1428:1438	The multivariate Cox proportional hazards model for age, Eastern Cooperative Oncology Group performance score, and bleeding risk factors revealed the rivaroxaban group showed a 1.176-fold composite event risk without statistical significance (0.595-2.324, p = 0.641).
31096241	9	57	theme	rivaroxaban	1563:1573	arg1	group					1575:1579	the rivaroxaban group	1559:1579	the rivaroxaban group	1559:1579	There was no statistically significant intergroup difference for the incidence of VTE recurrence (5.3% in the rivaroxaban group versus 2.7% in the dalteparin group, p = 0.495) and major or clinically relevant nonmajor bleeding (23.7% in the rivaroxaban group versus 13.7% in the dalteparin group, p = 0.089).
31096241	12	58	theme	cancer-associated	2062:2078	arg1	VTE					2080:2082	lung cancer-associated VTE	2057:2082	lung cancer-associated VTE	2057:2082	Therefore, rivaroxaban may be a valuable treatment option for lung cancer-associated VTE.
31096241	7	59	from	patients	1108:1115	arg1	groups					1163:1168	the rivaroxaban and dalteparin (p = 0.045) groups	1120:1168	the rivaroxaban and dalteparin (p = 0.045) groups	1120:1168	RESULTS The composite event occurred in 38 (29.0) and 12 (16.4%) patients in the rivaroxaban and dalteparin (p = 0.045) groups, respectively.
31096241	8	60	theme	statistical	1402:1412	arg1	significance					1414:1425	statistical significance	1402:1425	statistical significance	1402:1425	The multivariate Cox proportional hazards model for age, Eastern Cooperative Oncology Group performance score, and bleeding risk factors revealed the rivaroxaban group showed a 1.176-fold composite event risk without statistical significance (0.595-2.324, p = 0.641).
31096241	3	61	theme	lung	559:562	arg1	cancer					564:569	primary lung cancer	551:569	primary lung cancer	551:569	OBJECTIVES We examined the efficacy and safety of rivaroxaban compared with dalteparin for cancer-associated VTE in patients with primary lung cancer.
31096241	9	62	from	%	1685:1685	arg1	group					1706:1710	the rivaroxaban group	1690:1710	the rivaroxaban group	1690:1710	There was no statistically significant intergroup difference for the incidence of VTE recurrence (5.3% in the rivaroxaban group versus 2.7% in the dalteparin group, p = 0.495) and major or clinically relevant nonmajor bleeding (23.7% in the rivaroxaban group versus 13.7% in the dalteparin group, p = 0.089).
31096241	0	63	from	Patients	128:135	arg1	Treatment					89:97	Long-Term Treatment	79:97	Long-Term Treatment of Venous Thromboembolism in Patients with Lung Cancer	79:152	A Retrospective Study on Efficacy and Safety of Rivaroxaban and Dalteparin for Long-Term Treatment of Venous Thromboembolism in Patients with Lung Cancer.
31096241	12	64	theme	treatment	2036:2044	arg1	rivaroxaban					2006:2016	rivaroxaban	2006:2016	rivaroxaban	2006:2016	Therefore, rivaroxaban may be a valuable treatment option for lung cancer-associated VTE.
31096241	12	64	theme	treatment	2036:2044	arg1	option					2046:2051	a valuable treatment option	2025:2051	a valuable treatment option for lung cancer-associated VTE	2025:2082	Therefore, rivaroxaban may be a valuable treatment option for lung cancer-associated VTE.
31096241	1	65	theme	low-molecular-weight	237:256	arg1	heparin					258:264	low-molecular-weight heparin	237:264	low-molecular-weight heparin	237:264	BACKGROUND Standard therapy for cancer-associated venous thromboembolism (VTE) is low-molecular-weight heparin.
31096241	9	66	dep	=	1620:1620	arg1	%					1591:1591	2.7%	1588:1591	2.7% in the dalteparin group	1588:1615	There was no statistically significant intergroup difference for the incidence of VTE recurrence (5.3% in the rivaroxaban group versus 2.7% in the dalteparin group, p = 0.495) and major or clinically relevant nonmajor bleeding (23.7% in the rivaroxaban group versus 13.7% in the dalteparin group, p = 0.089).
31096241	9	66	dep	=	1620:1620	arg1	%					1554:1554	5.3%	1551:1554	5.3% in the rivaroxaban group	1551:1579	There was no statistically significant intergroup difference for the incidence of VTE recurrence (5.3% in the rivaroxaban group versus 2.7% in the dalteparin group, p = 0.495) and major or clinically relevant nonmajor bleeding (23.7% in the rivaroxaban group versus 13.7% in the dalteparin group, p = 0.089).
31096241	4	67	theme	lung	645:648	arg1	cancer					650:655	primary lung cancer	637:655	primary lung cancer	637:655	METHODS A single-center retrospective study of 204 patients with primary lung cancer who were prescribed rivaroxaban (n = 131) or dalteparin (n = 73) for VTE was performed.
31096241	10	68	theme	mortality	1815:1823	arg1	rate					1825:1828	the all-cause mortality rate	1801:1828	the all-cause mortality rate (hazard ratio 0.864, 95% CI 0.624-1.196, p = 0.337)	1801:1880	There was no significant difference in the all-cause mortality rate (hazard ratio 0.864, 95% CI 0.624-1.196, p = 0.337).
31096241	3	69	with	patients	537:544	arg1	cancer					564:569	primary lung cancer	551:569	primary lung cancer	551:569	OBJECTIVES We examined the efficacy and safety of rivaroxaban compared with dalteparin for cancer-associated VTE in patients with primary lung cancer.
31096241	7	70	theme	12	1097:1098	arg1	patients					1108:1115	38 (29.0) and 12 (16.4%) patients	1083:1115	38 (29.0) and 12 (16.4%) patients in the rivaroxaban and dalteparin (p = 0.045) groups	1083:1168	RESULTS The composite event occurred in 38 (29.0) and 12 (16.4%) patients in the rivaroxaban and dalteparin (p = 0.045) groups, respectively.
31096241	2	71	theme	oral	285:288	arg1	anticoagulants					290:303	direct oral anticoagulants	278:303	direct oral anticoagulants	278:303	The use of direct oral anticoagulants for cancer-associated VTE has increased; however, their efficacy and safety in lung cancer patients remain unclear.
31096241	1	72	theme	Standard	166:173	arg1	therapy					175:181	BACKGROUND Standard therapy	155:181	BACKGROUND Standard therapy for cancer-associated venous thromboembolism (VTE)	155:232	BACKGROUND Standard therapy for cancer-associated venous thromboembolism (VTE) is low-molecular-weight heparin.
31096241	8	73	theme	Cox	1202:1204	arg1	model					1227:1231	The multivariate Cox proportional hazards model	1185:1231	The multivariate Cox proportional hazards model for age, Eastern Cooperative Oncology Group performance score, and bleeding risk factors	1185:1320	The multivariate Cox proportional hazards model for age, Eastern Cooperative Oncology Group performance score, and bleeding risk factors revealed the rivaroxaban group showed a 1.176-fold composite event risk without statistical significance (0.595-2.324, p = 0.641).
31096241	8	74	theme	hazards	1219:1225	arg1	model					1227:1231	The multivariate Cox proportional hazards model	1185:1231	The multivariate Cox proportional hazards model for age, Eastern Cooperative Oncology Group performance score, and bleeding risk factors	1185:1320	The multivariate Cox proportional hazards model for age, Eastern Cooperative Oncology Group performance score, and bleeding risk factors revealed the rivaroxaban group showed a 1.176-fold composite event risk without statistical significance (0.595-2.324, p = 0.641).
31096241	10	75	theme	%	1853:1853	arg1	CI					1855:1856	95% CI 0.624-1.196	1851:1868	95% CI 0.624-1.196	1851:1868	There was no significant difference in the all-cause mortality rate (hazard ratio 0.864, 95% CI 0.624-1.196, p = 0.337).
31096241	12	76	theme	lung	2057:2060	arg1	VTE					2080:2082	lung cancer-associated VTE	2057:2082	lung cancer-associated VTE	2057:2082	Therefore, rivaroxaban may be a valuable treatment option for lung cancer-associated VTE.
31096241	1	77	theme	venous	205:210	arg1	thromboembolism					212:226	cancer-associated venous thromboembolism	187:226	cancer-associated venous thromboembolism (VTE)	187:232	BACKGROUND Standard therapy for cancer-associated venous thromboembolism (VTE) is low-molecular-weight heparin.
31096241	1	77	theme	venous	205:210	arg1	VTE					229:231	VTE	229:231	VTE	229:231	BACKGROUND Standard therapy for cancer-associated venous thromboembolism (VTE) is low-molecular-weight heparin.
31096241	6	78	theme	all-cause	968:976	arg1	mortality					978:986	major and clinically relevant nonmajor bleeding, all-cause mortality	919:986	mortality	978:986	Secondary endpoints included the incidence of recurrence, major and clinically relevant nonmajor bleeding, all-cause mortality, and bleeding or pulmonary embolism-related mortality.
31096241	4	79	theme	=	692:692	arg1	n					690:690	n = 131	690:696	n = 131	690:696	METHODS A single-center retrospective study of 204 patients with primary lung cancer who were prescribed rivaroxaban (n = 131) or dalteparin (n = 73) for VTE was performed.
31096241	4	79	theme	=	692:692	arg1	rivaroxaban					677:687	rivaroxaban	677:687	rivaroxaban (n = 131)	677:697	METHODS A single-center retrospective study of 204 patients with primary lung cancer who were prescribed rivaroxaban (n = 131) or dalteparin (n = 73) for VTE was performed.
31096241	5	80	theme	relevant	833:840	arg1	nonmajor					842:849	major or clinically relevant nonmajor bleeding	813:858	major or clinically relevant nonmajor bleeding	813:858	The primary endpoint was a composite event including recurrence and major or clinically relevant nonmajor bleeding.
31096241	6	81	theme	bleeding	958:965	arg1	mortality					978:986	major and clinically relevant nonmajor bleeding, all-cause mortality	919:986	mortality	978:986	Secondary endpoints included the incidence of recurrence, major and clinically relevant nonmajor bleeding, all-cause mortality, and bleeding or pulmonary embolism-related mortality.
31096241	6	82	theme	relevant	940:947	arg1	mortality					978:986	major and clinically relevant nonmajor bleeding, all-cause mortality	919:986	mortality	978:986	Secondary endpoints included the incidence of recurrence, major and clinically relevant nonmajor bleeding, all-cause mortality, and bleeding or pulmonary embolism-related mortality.
31096241	11	83	theme	safety	1926:1931	arg1	profile					1946:1952	the safety and efficacy profile	1922:1952	the safety and efficacy profile of rivaroxaban	1922:1967	CONCLUSIONS There was no difference in the safety and efficacy profile of rivaroxaban compared with dalteparin.
31096241	8	84	theme	Cooperative	1250:1260	arg1	score					1289:1293	Eastern Cooperative Oncology Group performance score	1242:1293	Eastern Cooperative Oncology Group performance score	1242:1293	The multivariate Cox proportional hazards model for age, Eastern Cooperative Oncology Group performance score, and bleeding risk factors revealed the rivaroxaban group showed a 1.176-fold composite event risk without statistical significance (0.595-2.324, p = 0.641).
31096241	4	85	with	patients	623:630	arg1	cancer					650:655	primary lung cancer	637:655	primary lung cancer	637:655	METHODS A single-center retrospective study of 204 patients with primary lung cancer who were prescribed rivaroxaban (n = 131) or dalteparin (n = 73) for VTE was performed.
31096241	0	86	from	Treatment	89:97	arg1	Patients					128:135	Patients	128:135	Patients with Lung Cancer	128:152	A Retrospective Study on Efficacy and Safety of Rivaroxaban and Dalteparin for Long-Term Treatment of Venous Thromboembolism in Patients with Lung Cancer.
31096241	8	87	theme	Group	1271:1275	arg1	score					1289:1293	Eastern Cooperative Oncology Group performance score	1242:1293	Eastern Cooperative Oncology Group performance score	1242:1293	The multivariate Cox proportional hazards model for age, Eastern Cooperative Oncology Group performance score, and bleeding risk factors revealed the rivaroxaban group showed a 1.176-fold composite event risk without statistical significance (0.595-2.324, p = 0.641).
31096241	9	88	theme	intergroup	1492:1501	arg1	difference					1503:1512	no statistically significant intergroup difference	1463:1512	no statistically significant intergroup difference	1463:1512	There was no statistically significant intergroup difference for the incidence of VTE recurrence (5.3% in the rivaroxaban group versus 2.7% in the dalteparin group, p = 0.495) and major or clinically relevant nonmajor bleeding (23.7% in the rivaroxaban group versus 13.7% in the dalteparin group, p = 0.089).
31096241	6	89	theme	mortality	1032:1040	arg1	incidence					894:902	the incidence	890:902	the incidence of recurrence, major and clinically relevant nonmajor bleeding, all-cause mortality, and bleeding or pulmonary embolism-related mortality	890:1040	Secondary endpoints included the incidence of recurrence, major and clinically relevant nonmajor bleeding, all-cause mortality, and bleeding or pulmonary embolism-related mortality.
31096241	5	90	theme	primary	749:755	arg1	event					782:786	a composite event	770:786	a composite event including recurrence and major or clinically relevant nonmajor bleeding	770:858	The primary endpoint was a composite event including recurrence and major or clinically relevant nonmajor bleeding.
31096241	5	90	theme	primary	749:755	arg1	endpoint					757:764	The primary endpoint	745:764	The primary endpoint	745:764	The primary endpoint was a composite event including recurrence and major or clinically relevant nonmajor bleeding.
31096241	6	91	theme	pulmonary	1005:1013	arg1	mortality					1032:1040	bleeding or pulmonary embolism-related mortality	993:1040	bleeding or pulmonary embolism-related mortality	993:1040	Secondary endpoints included the incidence of recurrence, major and clinically relevant nonmajor bleeding, all-cause mortality, and bleeding or pulmonary embolism-related mortality.
31096241	9	92	theme	rivaroxaban	1694:1704	arg1	group					1706:1710	the rivaroxaban group	1690:1710	the rivaroxaban group	1690:1710	There was no statistically significant intergroup difference for the incidence of VTE recurrence (5.3% in the rivaroxaban group versus 2.7% in the dalteparin group, p = 0.495) and major or clinically relevant nonmajor bleeding (23.7% in the rivaroxaban group versus 13.7% in the dalteparin group, p = 0.089).
31096241	10	93	dep	=	1873:1873	arg1	CI					1855:1856	95% CI 0.624-1.196	1851:1868	95% CI 0.624-1.196	1851:1868	There was no significant difference in the all-cause mortality rate (hazard ratio 0.864, 95% CI 0.624-1.196, p = 0.337).
31096241	10	93	dep	=	1873:1873	arg1	ratio					1838:1842	hazard ratio 0.864	1831:1848	hazard ratio 0.864	1831:1848	There was no significant difference in the all-cause mortality rate (hazard ratio 0.864, 95% CI 0.624-1.196, p = 0.337).
31096241	6	94	theme	bleeding	993:1000	arg1	mortality					1032:1040	bleeding or pulmonary embolism-related mortality	993:1040	bleeding or pulmonary embolism-related mortality	993:1040	Secondary endpoints included the incidence of recurrence, major and clinically relevant nonmajor bleeding, all-cause mortality, and bleeding or pulmonary embolism-related mortality.
31096241	8	95	theme	risk	1309:1312	arg1	factors					1314:1320	bleeding risk factors	1300:1320	bleeding risk factors	1300:1320	The multivariate Cox proportional hazards model for age, Eastern Cooperative Oncology Group performance score, and bleeding risk factors revealed the rivaroxaban group showed a 1.176-fold composite event risk without statistical significance (0.595-2.324, p = 0.641).
31096241	0	96	theme	Venous	102:107	arg1	Thromboembolism					109:123	Venous Thromboembolism	102:123	Venous Thromboembolism in Patients with Lung Cancer	102:152	A Retrospective Study on Efficacy and Safety of Rivaroxaban and Dalteparin for Long-Term Treatment of Venous Thromboembolism in Patients with Lung Cancer.
31096241	9	97	theme	VTE	1535:1537	arg1	recurrence					1539:1548	VTE recurrence (5.3% in the rivaroxaban group versus 2.7% in the dalteparin group, p = 0.495) and major or clinically relevant nonmajor bleeding (23.7% in the rivaroxaban group versus 13.7% in the dalteparin group, p = 0.089)	1535:1759	recurrence	1539:1548	There was no statistically significant intergroup difference for the incidence of VTE recurrence (5.3% in the rivaroxaban group versus 2.7% in the dalteparin group, p = 0.495) and major or clinically relevant nonmajor bleeding (23.7% in the rivaroxaban group versus 13.7% in the dalteparin group, p = 0.089).
31096241	0	98	theme	Retrospective	2:14	arg1	Study					16:20	A Retrospective Study	0:20	A Retrospective Study on Efficacy and Safety of Rivaroxaban and Dalteparin for Long-Term Treatment of Venous Thromboembolism in Patients with Lung Cancer.	0:153	A Retrospective Study on Efficacy and Safety of Rivaroxaban and Dalteparin for Long-Term Treatment of Venous Thromboembolism in Patients with Lung Cancer.
31096241	4	99	dep	METHODS	572:578	arg1	study					610:614	A single-center retrospective study	580:614	METHODS A single-center retrospective study of 204 patients with primary lung cancer who were prescribed rivaroxaban (n = 131) or dalteparin (n = 73) for VTE	572:728	METHODS A single-center retrospective study of 204 patients with primary lung cancer who were prescribed rivaroxaban (n = 131) or dalteparin (n = 73) for VTE was performed.
31096241	9	100	from	%	1591:1591	arg1	group					1611:1615	the dalteparin group	1596:1615	the dalteparin group	1596:1615	There was no statistically significant intergroup difference for the incidence of VTE recurrence (5.3% in the rivaroxaban group versus 2.7% in the dalteparin group, p = 0.495) and major or clinically relevant nonmajor bleeding (23.7% in the rivaroxaban group versus 13.7% in the dalteparin group, p = 0.089).
31096241	8	101	theme	rivaroxaban	1335:1345	arg1	group					1347:1351	the rivaroxaban group	1331:1351	the rivaroxaban group	1331:1351	The multivariate Cox proportional hazards model for age, Eastern Cooperative Oncology Group performance score, and bleeding risk factors revealed the rivaroxaban group showed a 1.176-fold composite event risk without statistical significance (0.595-2.324, p = 0.641).
31096241	2	102	theme	lung	384:387	arg1	patients					396:403	lung cancer patients	384:403	lung cancer patients	384:403	The use of direct oral anticoagulants for cancer-associated VTE has increased; however, their efficacy and safety in lung cancer patients remain unclear.
31096241	7	103	theme	dalteparin	1140:1149	arg1	groups					1163:1168	the rivaroxaban and dalteparin (p = 0.045) groups	1120:1168	the rivaroxaban and dalteparin (p = 0.045) groups	1120:1168	RESULTS The composite event occurred in 38 (29.0) and 12 (16.4%) patients in the rivaroxaban and dalteparin (p = 0.045) groups, respectively.
31096241	4	104	theme	retrospective	596:608	arg1	study					610:614	A single-center retrospective study	580:614	METHODS A single-center retrospective study of 204 patients with primary lung cancer who were prescribed rivaroxaban (n = 131) or dalteparin (n = 73) for VTE	572:728	METHODS A single-center retrospective study of 204 patients with primary lung cancer who were prescribed rivaroxaban (n = 131) or dalteparin (n = 73) for VTE was performed.
31096241	7	105	theme	rivaroxaban	1124:1134	arg1	groups					1163:1168	the rivaroxaban and dalteparin (p = 0.045) groups	1120:1168	the rivaroxaban and dalteparin (p = 0.045) groups	1120:1168	RESULTS The composite event occurred in 38 (29.0) and 12 (16.4%) patients in the rivaroxaban and dalteparin (p = 0.045) groups, respectively.
31096241	6	106	theme	Secondary	861:869	arg1	endpoints					871:879	Secondary endpoints	861:879	Secondary endpoints	861:879	Secondary endpoints included the incidence of recurrence, major and clinically relevant nonmajor bleeding, all-cause mortality, and bleeding or pulmonary embolism-related mortality.
31096241	8	107	theme	1.176-fold	1362:1371	arg1	event					1383:1387	a 1.176-fold composite event	1360:1387	a 1.176-fold composite event risk	1360:1392	The multivariate Cox proportional hazards model for age, Eastern Cooperative Oncology Group performance score, and bleeding risk factors revealed the rivaroxaban group showed a 1.176-fold composite event risk without statistical significance (0.595-2.324, p = 0.641).
31096241	5	108	theme	composite	772:780	arg1	event					782:786	a composite event	770:786	a composite event including recurrence and major or clinically relevant nonmajor bleeding	770:858	The primary endpoint was a composite event including recurrence and major or clinically relevant nonmajor bleeding.
31096241	5	108	theme	composite	772:780	arg1	endpoint					757:764	The primary endpoint	745:764	The primary endpoint	745:764	The primary endpoint was a composite event including recurrence and major or clinically relevant nonmajor bleeding.
31096241	3	109	dep	efficacy	448:455	arg1	the					444:446	the	444:446	the	444:446	OBJECTIVES We examined the efficacy and safety of rivaroxaban compared with dalteparin for cancer-associated VTE in patients with primary lung cancer.
31096241	10	110	dep	rate	1825:1828	arg1	=					1873:1873	=	1873:1873	=	1873:1873	There was no significant difference in the all-cause mortality rate (hazard ratio 0.864, 95% CI 0.624-1.196, p = 0.337).
31096241	8	111	theme	event	1383:1387	arg1	risk					1389:1392	a 1.176-fold composite event risk	1360:1392	a 1.176-fold composite event risk	1360:1392	The multivariate Cox proportional hazards model for age, Eastern Cooperative Oncology Group performance score, and bleeding risk factors revealed the rivaroxaban group showed a 1.176-fold composite event risk without statistical significance (0.595-2.324, p = 0.641).
31096241	3	112	theme	cancer-associated	512:528	arg1	VTE					530:532	cancer-associated VTE	512:532	cancer-associated VTE in patients with primary lung cancer	512:569	OBJECTIVES We examined the efficacy and safety of rivaroxaban compared with dalteparin for cancer-associated VTE in patients with primary lung cancer.
31096241	12	113	theme	valuable	2027:2034	arg1	rivaroxaban					2006:2016	rivaroxaban	2006:2016	rivaroxaban	2006:2016	Therefore, rivaroxaban may be a valuable treatment option for lung cancer-associated VTE.
31096241	12	113	theme	valuable	2027:2034	arg1	option					2046:2051	a valuable treatment option	2025:2051	a valuable treatment option for lung cancer-associated VTE	2025:2082	Therefore, rivaroxaban may be a valuable treatment option for lung cancer-associated VTE.
31096241	4	114	theme	primary	637:643	arg1	cancer					650:655	primary lung cancer	637:655	primary lung cancer	637:655	METHODS A single-center retrospective study of 204 patients with primary lung cancer who were prescribed rivaroxaban (n = 131) or dalteparin (n = 73) for VTE was performed.
31096241	10	115	theme	significant	1775:1785	arg1	difference					1787:1796	no significant difference	1772:1796	no significant difference	1772:1796	There was no significant difference in the all-cause mortality rate (hazard ratio 0.864, 95% CI 0.624-1.196, p = 0.337).
31096241	0	116	from	Thromboembolism	109:123	arg1	Patients					128:135	Patients	128:135	Patients with Lung Cancer	128:152	A Retrospective Study on Efficacy and Safety of Rivaroxaban and Dalteparin for Long-Term Treatment of Venous Thromboembolism in Patients with Lung Cancer.
31096241	7	117	theme	p	1152:1152	arg1	rivaroxaban					1124:1134	rivaroxaban	1124:1134	rivaroxaban	1124:1134	RESULTS The composite event occurred in 38 (29.0) and 12 (16.4%) patients in the rivaroxaban and dalteparin (p = 0.045) groups, respectively.
31096241	7	117	theme	p	1152:1152	arg1	0.045					1156:1160	p = 0.045	1152:1160	p = 0.045	1152:1160	RESULTS The composite event occurred in 38 (29.0) and 12 (16.4%) patients in the rivaroxaban and dalteparin (p = 0.045) groups, respectively.
30187428	0	0	theme	Broiler	166:172	arg1	Chickens					174:181	Broiler Chickens	166:181	Broiler Chickens	166:181	Effects of Dietary Supplementation of Probiotic Mix and Prebiotic on Growth Performance, Cecal Microbiota Composition, and Protection Against Escherichia coli O78 in Broiler Chickens.
30187428	1	1	theme	multi-strain	244:255	arg1	mix					278:280	a multi-strain commercial probiotic mix	242:280	a multi-strain commercial probiotic mix	242:280	The current study conducted to investigate the effects of a multi-strain commercial probiotic mix and prebiotic (isomaltooligosaccharide, IMO) on broiler performance parameters, cecal microbiota composition, and protection against challenge with avian pathogenic Escherichia coli (APEC) O78.
30187428	6	2	theme	treated	1332:1338	arg1	broilers					1340:1347	treated broilers	1332:1347	treated broilers	1332:1347	The probiotic mix and IMO significantly increased the total lactobacilli and total lactobacilli-enterococci populations in the ceca of treated broilers, respectively compared with those in the control groups.
30187428	1	3	theme	probiotic	268:276	arg1	mix					278:280	a multi-strain commercial probiotic mix	242:280	a multi-strain commercial probiotic mix	242:280	The current study conducted to investigate the effects of a multi-strain commercial probiotic mix and prebiotic (isomaltooligosaccharide, IMO) on broiler performance parameters, cecal microbiota composition, and protection against challenge with avian pathogenic Escherichia coli (APEC) O78.
30187428	2	4	theme	basal	598:602	arg1	diet					604:607	basal diet	598:607	basal diet and challenged with E. coli O78 at 28 days old, G03: basal diet with probiotic mix and challenged with E. coli O78 at 28 days old, and G04	598:746	For this purpose, 101-day-old Cobb chicks were randomly allocated into four experimental groups (G)-G01: basal diet, G02: basal diet and challenged with E. coli O78 at 28 days old, G03: basal diet with probiotic mix and challenged with E. coli O78 at 28 days old, and G04: basal diet with IMO and challenged with E. coli O78 at 28 days old.
30187428	4	5	theme	fourth	965:970	arg1	week					982:985	the fourth and fifth week	961:985	the fourth and fifth week	961:985	The body gain at the fourth and fifth week was higher (P < 0.05) in G03 than those of the other groups.
30187428	4	6	dep	higher	991:996	arg1	P < 0.05					999:1006	P < 0.05	999:1006	P < 0.05	999:1006	The body gain at the fourth and fifth week was higher (P < 0.05) in G03 than those of the other groups.
30187428	2	7	theme	probiotic	678:686	arg1	mix					688:690	probiotic mix	678:690	probiotic mix	678:690	For this purpose, 101-day-old Cobb chicks were randomly allocated into four experimental groups (G)-G01: basal diet, G02: basal diet and challenged with E. coli O78 at 28 days old, G03: basal diet with probiotic mix and challenged with E. coli O78 at 28 days old, and G04: basal diet with IMO and challenged with E. coli O78 at 28 days old.
30187428	8	8	theme	intestinal	1654:1663	arg1	microbiota					1665:1674	the intestinal microbiota	1650:1674	the intestinal microbiota of broiler chickens challenged with APEC O78	1650:1719	It was concluded that probiotic mix or IMO significantly improved the growth performance and modulated the intestinal microbiota of broiler chickens challenged with APEC O78.
30187428	2	9	theme	experimental	552:563	arg1	groups					565:570	four experimental groups	547:570	four experimental groups (G)-G01: basal diet, G02: basal diet and challenged with E. coli O78 at 28 days old, G03: basal diet with probiotic mix and challenged with E. coli O78 at 28 days old, and G04: basal diet with IMO	547:767	For this purpose, 101-day-old Cobb chicks were randomly allocated into four experimental groups (G)-G01: basal diet, G02: basal diet and challenged with E. coli O78 at 28 days old, G03: basal diet with probiotic mix and challenged with E. coli O78 at 28 days old, and G04: basal diet with IMO and challenged with E. coli O78 at 28 days old.
30187428	4	10	theme	body	948:951	arg1	gain					953:956	The body gain	944:956	The body gain at the fourth and fifth week	944:985	The body gain at the fourth and fifth week was higher (P < 0.05) in G03 than those of the other groups.
30187428	4	10	theme	body	948:951	arg1	higher					991:996	higher	991:996	higher	991:996	The body gain at the fourth and fifth week was higher (P < 0.05) in G03 than those of the other groups.
30187428	3	11	dep	heavier	869:875	arg1	P < 0.05					878:885	P < 0.05	878:885	P < 0.05	878:885	Results showed that weekly body weights in G03 were heavier (P < 0.05) than those of G01 and G02 at the fourth and fifth week.
30187428	2	12	theme	E.	789:790	arg1	O78					797:799	E. coli O78	789:799	E. coli O78 at 28 days old	789:814	For this purpose, 101-day-old Cobb chicks were randomly allocated into four experimental groups (G)-G01: basal diet, G02: basal diet and challenged with E. coli O78 at 28 days old, G03: basal diet with probiotic mix and challenged with E. coli O78 at 28 days old, and G04: basal diet with IMO and challenged with E. coli O78 at 28 days old.
30187428	2	13	dep	diet	587:590	arg1	diet					604:607	basal diet	598:607	basal diet and challenged with E. coli O78 at 28 days old, G03: basal diet with probiotic mix and challenged with E. coli O78 at 28 days old, and G04	598:746	For this purpose, 101-day-old Cobb chicks were randomly allocated into four experimental groups (G)-G01: basal diet, G02: basal diet and challenged with E. coli O78 at 28 days old, G03: basal diet with probiotic mix and challenged with E. coli O78 at 28 days old, and G04: basal diet with IMO and challenged with E. coli O78 at 28 days old.
30187428	2	13	dep	diet	587:590	arg1	diet					755:758	basal diet	749:758	basal diet with IMO	749:767	For this purpose, 101-day-old Cobb chicks were randomly allocated into four experimental groups (G)-G01: basal diet, G02: basal diet and challenged with E. coli O78 at 28 days old, G03: basal diet with probiotic mix and challenged with E. coli O78 at 28 days old, and G04: basal diet with IMO and challenged with E. coli O78 at 28 days old.
30187428	0	14	theme	Escherichia	142:152	arg1	O78					159:161	Escherichia coli O78	142:161	Escherichia coli O78 in Broiler Chickens	142:181	Effects of Dietary Supplementation of Probiotic Mix and Prebiotic on Growth Performance, Cecal Microbiota Composition, and Protection Against Escherichia coli O78 in Broiler Chickens.
30187428	0	15	dep	Escherichia	142:152	arg1	coli					154:157	coli	154:157	coli	154:157	Effects of Dietary Supplementation of Probiotic Mix and Prebiotic on Growth Performance, Cecal Microbiota Composition, and Protection Against Escherichia coli O78 in Broiler Chickens.
30187428	2	16	theme	101-day-old	494:504	arg1	chicks					511:516	101-day-old Cobb chicks	494:516	101-day-old Cobb chicks	494:516	For this purpose, 101-day-old Cobb chicks were randomly allocated into four experimental groups (G)-G01: basal diet, G02: basal diet and challenged with E. coli O78 at 28 days old, G03: basal diet with probiotic mix and challenged with E. coli O78 at 28 days old, and G04: basal diet with IMO and challenged with E. coli O78 at 28 days old.
30187428	0	17	theme	Prebiotic	56:64	arg1	Supplementation					19:33	Dietary Supplementation	11:33	Dietary Supplementation of Probiotic Mix and Prebiotic	11:64	Effects of Dietary Supplementation of Probiotic Mix and Prebiotic on Growth Performance, Cecal Microbiota Composition, and Protection Against Escherichia coli O78 in Broiler Chickens.
30187428	0	18	theme	Growth	69:74	arg1	Performance					76:86	Growth Performance	69:86	Growth Performance	69:86	Effects of Dietary Supplementation of Probiotic Mix and Prebiotic on Growth Performance, Cecal Microbiota Composition, and Protection Against Escherichia coli O78 in Broiler Chickens.
30187428	1	19	theme	performance	338:348	arg1	parameters					350:359	broiler performance parameters	330:359	broiler performance parameters	330:359	The current study conducted to investigate the effects of a multi-strain commercial probiotic mix and prebiotic (isomaltooligosaccharide, IMO) on broiler performance parameters, cecal microbiota composition, and protection against challenge with avian pathogenic Escherichia coli (APEC) O78.
30187428	0	20	from	Effects	0:6	arg1	Protection					123:132	Protection	123:132	Protection Against Escherichia coli O78 in Broiler Chickens	123:181	Effects of Dietary Supplementation of Probiotic Mix and Prebiotic on Growth Performance, Cecal Microbiota Composition, and Protection Against Escherichia coli O78 in Broiler Chickens.
30187428	0	20	from	Effects	0:6	arg1	Performance					76:86	Growth Performance	69:86	Growth Performance	69:86	Effects of Dietary Supplementation of Probiotic Mix and Prebiotic on Growth Performance, Cecal Microbiota Composition, and Protection Against Escherichia coli O78 in Broiler Chickens.
30187428	0	20	from	Effects	0:6	arg1	Composition					106:116	Cecal Microbiota Composition	89:116	Cecal Microbiota Composition	89:116	Effects of Dietary Supplementation of Probiotic Mix and Prebiotic on Growth Performance, Cecal Microbiota Composition, and Protection Against Escherichia coli O78 in Broiler Chickens.
30187428	2	21	from	28 days	727:733	arg1	G04					744:746	G04	744:746	G04	744:746	For this purpose, 101-day-old Cobb chicks were randomly allocated into four experimental groups (G)-G01: basal diet, G02: basal diet and challenged with E. coli O78 at 28 days old, G03: basal diet with probiotic mix and challenged with E. coli O78 at 28 days old, and G04: basal diet with IMO and challenged with E. coli O78 at 28 days old.
30187428	2	21	from	28 days	727:733	arg1	O78					720:722	E. coli O78	712:722	E. coli O78 at 28 days old	712:737	For this purpose, 101-day-old Cobb chicks were randomly allocated into four experimental groups (G)-G01: basal diet, G02: basal diet and challenged with E. coli O78 at 28 days old, G03: basal diet with probiotic mix and challenged with E. coli O78 at 28 days old, and G04: basal diet with IMO and challenged with E. coli O78 at 28 days old.
30187428	2	22	with	diet	755:758	arg1	IMO					765:767	IMO	765:767	IMO	765:767	For this purpose, 101-day-old Cobb chicks were randomly allocated into four experimental groups (G)-G01: basal diet, G02: basal diet and challenged with E. coli O78 at 28 days old, G03: basal diet with probiotic mix and challenged with E. coli O78 at 28 days old, and G04: basal diet with IMO and challenged with E. coli O78 at 28 days old.
30187428	5	23	dep	groups	1175:1180	arg1	G02					1191:1193	G02	1191:1193	G02	1191:1193	The hot carcass weight (g) was significantly higher in broiler chickens kept in G03 and G04 compared with those in the control groups (G01 and G02).
30187428	5	23	dep	groups	1175:1180	arg1	G01					1183:1185	G01	1183:1185	G01	1183:1185	The hot carcass weight (g) was significantly higher in broiler chickens kept in G03 and G04 compared with those in the control groups (G01 and G02).
30187428	5	23	dep	groups	1175:1180	arg1	groups					1175:1180	the control groups	1163:1180	the control groups (G01 and G02)	1163:1194	The hot carcass weight (g) was significantly higher in broiler chickens kept in G03 and G04 compared with those in the control groups (G01 and G02).
30187428	0	24	theme	Microbiota	95:104	arg1	Composition					106:116	Cecal Microbiota Composition	89:116	Cecal Microbiota Composition	89:116	Effects of Dietary Supplementation of Probiotic Mix and Prebiotic on Growth Performance, Cecal Microbiota Composition, and Protection Against Escherichia coli O78 in Broiler Chickens.
30187428	1	25	theme	microbiota	368:377	arg1	composition					379:389	cecal microbiota composition	362:389	cecal microbiota composition	362:389	The current study conducted to investigate the effects of a multi-strain commercial probiotic mix and prebiotic (isomaltooligosaccharide, IMO) on broiler performance parameters, cecal microbiota composition, and protection against challenge with avian pathogenic Escherichia coli (APEC) O78.
30187428	1	26	with	challenge	415:423	arg1	O78					471:473	avian pathogenic Escherichia coli (APEC) O78	430:473	avian pathogenic Escherichia coli (APEC) O78	430:473	The current study conducted to investigate the effects of a multi-strain commercial probiotic mix and prebiotic (isomaltooligosaccharide, IMO) on broiler performance parameters, cecal microbiota composition, and protection against challenge with avian pathogenic Escherichia coli (APEC) O78.
30187428	7	27	dep	E.	1484:1485	arg1	coli					1487:1490	coli	1487:1490	coli	1487:1490	The treated broilers (G03 and G04) also showed lower mortality percentage and E. coli recovery rates the liver and spleen than those in G02.
30187428	6	28	theme	probiotic	1201:1209	arg1	mix					1211:1213	The probiotic mix	1197:1213	The probiotic mix	1197:1213	The probiotic mix and IMO significantly increased the total lactobacilli and total lactobacilli-enterococci populations in the ceca of treated broilers, respectively compared with those in the control groups.
30187428	1	29	theme	mix	278:280	arg1	effects					231:237	the effects	227:237	the effects of a multi-strain commercial probiotic mix and prebiotic (isomaltooligosaccharide, IMO) on broiler performance parameters, cecal microbiota composition, and protection against challenge with avian pathogenic Escherichia coli (APEC) O78	227:473	The current study conducted to investigate the effects of a multi-strain commercial probiotic mix and prebiotic (isomaltooligosaccharide, IMO) on broiler performance parameters, cecal microbiota composition, and protection against challenge with avian pathogenic Escherichia coli (APEC) O78.
30187428	7	30	theme	mortality	1459:1467	arg1	spleen					1521:1526	spleen	1521:1526	spleen	1521:1526	The treated broilers (G03 and G04) also showed lower mortality percentage and E. coli recovery rates the liver and spleen than those in G02.
30187428	7	30	theme	mortality	1459:1467	arg1	liver					1511:1515	liver	1511:1515	liver	1511:1515	The treated broilers (G03 and G04) also showed lower mortality percentage and E. coli recovery rates the liver and spleen than those in G02.
30187428	7	30	theme	mortality	1459:1467	arg1	rates					1501:1505	lower mortality percentage and E. coli recovery rates the liver and spleen	1453:1526	rates	1501:1505	The treated broilers (G03 and G04) also showed lower mortality percentage and E. coli recovery rates the liver and spleen than those in G02.
30187428	2	31	theme	E.	629:630	arg1	O78					637:639	E. coli O78	629:639	E. coli O78 at 28 days old, G03: basal diet with probiotic mix	629:690	For this purpose, 101-day-old Cobb chicks were randomly allocated into four experimental groups (G)-G01: basal diet, G02: basal diet and challenged with E. coli O78 at 28 days old, G03: basal diet with probiotic mix and challenged with E. coli O78 at 28 days old, and G04: basal diet with IMO and challenged with E. coli O78 at 28 days old.
30187428	5	32	theme	broiler	1103:1109	arg1	chickens					1111:1118	broiler chickens	1103:1118	broiler chickens kept in G03 and G04	1103:1138	The hot carcass weight (g) was significantly higher in broiler chickens kept in G03 and G04 compared with those in the control groups (G01 and G02).
30187428	0	33	theme	Dietary	11:17	arg1	Supplementation					19:33	Dietary Supplementation	11:33	Dietary Supplementation of Probiotic Mix and Prebiotic	11:64	Effects of Dietary Supplementation of Probiotic Mix and Prebiotic on Growth Performance, Cecal Microbiota Composition, and Protection Against Escherichia coli O78 in Broiler Chickens.
30187428	1	34	theme	prebiotic	286:294	arg1	effects					231:237	the effects	227:237	the effects of a multi-strain commercial probiotic mix and prebiotic (isomaltooligosaccharide, IMO) on broiler performance parameters, cecal microbiota composition, and protection against challenge with avian pathogenic Escherichia coli (APEC) O78	227:473	The current study conducted to investigate the effects of a multi-strain commercial probiotic mix and prebiotic (isomaltooligosaccharide, IMO) on broiler performance parameters, cecal microbiota composition, and protection against challenge with avian pathogenic Escherichia coli (APEC) O78.
30187428	6	35	from	populations	1305:1315	arg1	ceca					1324:1327	the ceca	1320:1327	the ceca of treated broilers	1320:1347	The probiotic mix and IMO significantly increased the total lactobacilli and total lactobacilli-enterococci populations in the ceca of treated broilers, respectively compared with those in the control groups.
30187428	2	36	dep	G03	657:659	arg1	diet					668:671	basal diet	662:671	G03: basal diet	657:671	For this purpose, 101-day-old Cobb chicks were randomly allocated into four experimental groups (G)-G01: basal diet, G02: basal diet and challenged with E. coli O78 at 28 days old, G03: basal diet with probiotic mix and challenged with E. coli O78 at 28 days old, and G04: basal diet with IMO and challenged with E. coli O78 at 28 days old.
30187428	2	37	dep	E.	712:713	arg1	coli					715:718	coli	715:718	coli	715:718	For this purpose, 101-day-old Cobb chicks were randomly allocated into four experimental groups (G)-G01: basal diet, G02: basal diet and challenged with E. coli O78 at 28 days old, G03: basal diet with probiotic mix and challenged with E. coli O78 at 28 days old, and G04: basal diet with IMO and challenged with E. coli O78 at 28 days old.
30187428	2	38	dep	E.	789:790	arg1	coli					792:795	coli	792:795	coli	792:795	For this purpose, 101-day-old Cobb chicks were randomly allocated into four experimental groups (G)-G01: basal diet, G02: basal diet and challenged with E. coli O78 at 28 days old, G03: basal diet with probiotic mix and challenged with E. coli O78 at 28 days old, and G04: basal diet with IMO and challenged with E. coli O78 at 28 days old.
30187428	5	39	theme	control	1167:1173	arg1	G02					1191:1193	G02	1191:1193	G02	1191:1193	The hot carcass weight (g) was significantly higher in broiler chickens kept in G03 and G04 compared with those in the control groups (G01 and G02).
30187428	5	39	theme	control	1167:1173	arg1	G01					1183:1185	G01	1183:1185	G01	1183:1185	The hot carcass weight (g) was significantly higher in broiler chickens kept in G03 and G04 compared with those in the control groups (G01 and G02).
30187428	5	39	theme	control	1167:1173	arg1	groups					1175:1180	the control groups	1163:1180	the control groups (G01 and G02)	1163:1194	The hot carcass weight (g) was significantly higher in broiler chickens kept in G03 and G04 compared with those in the control groups (G01 and G02).
30187428	0	40	theme	Mix	48:50	arg1	Supplementation					19:33	Dietary Supplementation	11:33	Dietary Supplementation of Probiotic Mix and Prebiotic	11:64	Effects of Dietary Supplementation of Probiotic Mix and Prebiotic on Growth Performance, Cecal Microbiota Composition, and Protection Against Escherichia coli O78 in Broiler Chickens.
30187428	3	41	theme	weekly	837:842	arg1	weights					849:855	weekly body weights	837:855	weekly body weights in G03	837:862	Results showed that weekly body weights in G03 were heavier (P < 0.05) than those of G01 and G02 at the fourth and fifth week.
30187428	6	42	theme	lactobacilli	1257:1268	arg1	populations					1305:1315	the total lactobacilli and total lactobacilli-enterococci populations	1247:1315	the total lactobacilli and total lactobacilli-enterococci populations in the ceca of treated broilers	1247:1347	The probiotic mix and IMO significantly increased the total lactobacilli and total lactobacilli-enterococci populations in the ceca of treated broilers, respectively compared with those in the control groups.
30187428	2	43	dep	groups	565:570	arg1	G02					593:595	G02	593:595	G02	593:595	For this purpose, 101-day-old Cobb chicks were randomly allocated into four experimental groups (G)-G01: basal diet, G02: basal diet and challenged with E. coli O78 at 28 days old, G03: basal diet with probiotic mix and challenged with E. coli O78 at 28 days old, and G04: basal diet with IMO and challenged with E. coli O78 at 28 days old.
30187428	2	43	dep	groups	565:570	arg1	diet					587:590	basal diet	581:590	basal diet	581:590	For this purpose, 101-day-old Cobb chicks were randomly allocated into four experimental groups (G)-G01: basal diet, G02: basal diet and challenged with E. coli O78 at 28 days old, G03: basal diet with probiotic mix and challenged with E. coli O78 at 28 days old, and G04: basal diet with IMO and challenged with E. coli O78 at 28 days old.
30187428	2	44	theme	basal	749:753	arg1	diet					755:758	basal diet	749:758	basal diet with IMO	749:767	For this purpose, 101-day-old Cobb chicks were randomly allocated into four experimental groups (G)-G01: basal diet, G02: basal diet and challenged with E. coli O78 at 28 days old, G03: basal diet with probiotic mix and challenged with E. coli O78 at 28 days old, and G04: basal diet with IMO and challenged with E. coli O78 at 28 days old.
30187428	8	45	theme	growth	1617:1622	arg1	performance					1624:1634	the growth performance	1613:1634	the growth performance	1613:1634	It was concluded that probiotic mix or IMO significantly improved the growth performance and modulated the intestinal microbiota of broiler chickens challenged with APEC O78.
30187428	7	46	dep	broilers	1418:1425	arg1	broilers					1418:1425	The treated broilers	1406:1425	The treated broilers (G03 and G04)	1406:1439	The treated broilers (G03 and G04) also showed lower mortality percentage and E. coli recovery rates the liver and spleen than those in G02.
30187428	7	46	dep	broilers	1418:1425	arg1	G04					1436:1438	G04	1436:1438	G04	1436:1438	The treated broilers (G03 and G04) also showed lower mortality percentage and E. coli recovery rates the liver and spleen than those in G02.
30187428	7	46	dep	broilers	1418:1425	arg1	G03					1428:1430	G03	1428:1430	G03	1428:1430	The treated broilers (G03 and G04) also showed lower mortality percentage and E. coli recovery rates the liver and spleen than those in G02.
30187428	2	47	theme	basal	581:585	arg1	G02					593:595	G02	593:595	G02	593:595	For this purpose, 101-day-old Cobb chicks were randomly allocated into four experimental groups (G)-G01: basal diet, G02: basal diet and challenged with E. coli O78 at 28 days old, G03: basal diet with probiotic mix and challenged with E. coli O78 at 28 days old, and G04: basal diet with IMO and challenged with E. coli O78 at 28 days old.
30187428	2	47	theme	basal	581:585	arg1	diet					587:590	basal diet	581:590	basal diet	581:590	For this purpose, 101-day-old Cobb chicks were randomly allocated into four experimental groups (G)-G01: basal diet, G02: basal diet and challenged with E. coli O78 at 28 days old, G03: basal diet with probiotic mix and challenged with E. coli O78 at 28 days old, and G04: basal diet with IMO and challenged with E. coli O78 at 28 days old.
30187428	0	48	from	O78	159:161	arg1	Chickens					174:181	Broiler Chickens	166:181	Broiler Chickens	166:181	Effects of Dietary Supplementation of Probiotic Mix and Prebiotic on Growth Performance, Cecal Microbiota Composition, and Protection Against Escherichia coli O78 in Broiler Chickens.
30187428	1	49	theme	coli	459:462	arg1	O78					471:473	avian pathogenic Escherichia coli (APEC) O78	430:473	avian pathogenic Escherichia coli (APEC) O78	430:473	The current study conducted to investigate the effects of a multi-strain commercial probiotic mix and prebiotic (isomaltooligosaccharide, IMO) on broiler performance parameters, cecal microbiota composition, and protection against challenge with avian pathogenic Escherichia coli (APEC) O78.
30187428	1	50	theme	commercial	257:266	arg1	mix					278:280	a multi-strain commercial probiotic mix	242:280	a multi-strain commercial probiotic mix	242:280	The current study conducted to investigate the effects of a multi-strain commercial probiotic mix and prebiotic (isomaltooligosaccharide, IMO) on broiler performance parameters, cecal microbiota composition, and protection against challenge with avian pathogenic Escherichia coli (APEC) O78.
30187428	2	51	from	28 days	804:810	arg1	O78					797:799	E. coli O78	789:799	E. coli O78 at 28 days old	789:814	For this purpose, 101-day-old Cobb chicks were randomly allocated into four experimental groups (G)-G01: basal diet, G02: basal diet and challenged with E. coli O78 at 28 days old, G03: basal diet with probiotic mix and challenged with E. coli O78 at 28 days old, and G04: basal diet with IMO and challenged with E. coli O78 at 28 days old.
30187428	4	52	theme	fifth	976:980	arg1	week					982:985	the fourth and fifth week	961:985	the fourth and fifth week	961:985	The body gain at the fourth and fifth week was higher (P < 0.05) in G03 than those of the other groups.
30187428	7	53	theme	recovery	1492:1499	arg1	spleen					1521:1526	spleen	1521:1526	spleen	1521:1526	The treated broilers (G03 and G04) also showed lower mortality percentage and E. coli recovery rates the liver and spleen than those in G02.
30187428	7	53	theme	recovery	1492:1499	arg1	liver					1511:1515	liver	1511:1515	liver	1511:1515	The treated broilers (G03 and G04) also showed lower mortality percentage and E. coli recovery rates the liver and spleen than those in G02.
30187428	7	53	theme	recovery	1492:1499	arg1	rates					1501:1505	lower mortality percentage and E. coli recovery rates the liver and spleen	1453:1526	rates	1501:1505	The treated broilers (G03 and G04) also showed lower mortality percentage and E. coli recovery rates the liver and spleen than those in G02.
30187428	4	54	from	week	982:985	arg1	gain					953:956	The body gain	944:956	The body gain at the fourth and fifth week	944:985	The body gain at the fourth and fifth week was higher (P < 0.05) in G03 than those of the other groups.
30187428	4	54	from	week	982:985	arg1	higher					991:996	higher	991:996	higher	991:996	The body gain at the fourth and fifth week was higher (P < 0.05) in G03 than those of the other groups.
30187428	2	55	from	28 days	644:650	arg1	O78					637:639	E. coli O78	629:639	E. coli O78 at 28 days old, G03: basal diet with probiotic mix	629:690	For this purpose, 101-day-old Cobb chicks were randomly allocated into four experimental groups (G)-G01: basal diet, G02: basal diet and challenged with E. coli O78 at 28 days old, G03: basal diet with probiotic mix and challenged with E. coli O78 at 28 days old, and G04: basal diet with IMO and challenged with E. coli O78 at 28 days old.
30187428	8	56	theme	broiler	1679:1685	arg1	chickens					1687:1694	broiler chickens	1679:1694	broiler chickens challenged with APEC O78	1679:1719	It was concluded that probiotic mix or IMO significantly improved the growth performance and modulated the intestinal microbiota of broiler chickens challenged with APEC O78.
30187428	1	57	theme	current	188:194	arg1	study					196:200	The current study	184:200	The current study	184:200	The current study conducted to investigate the effects of a multi-strain commercial probiotic mix and prebiotic (isomaltooligosaccharide, IMO) on broiler performance parameters, cecal microbiota composition, and protection against challenge with avian pathogenic Escherichia coli (APEC) O78.
30187428	7	58	theme	treated	1410:1416	arg1	broilers					1418:1425	The treated broilers	1406:1425	The treated broilers (G03 and G04)	1406:1439	The treated broilers (G03 and G04) also showed lower mortality percentage and E. coli recovery rates the liver and spleen than those in G02.
30187428	7	58	theme	treated	1410:1416	arg1	G04					1436:1438	G04	1436:1438	G04	1436:1438	The treated broilers (G03 and G04) also showed lower mortality percentage and E. coli recovery rates the liver and spleen than those in G02.
30187428	7	58	theme	treated	1410:1416	arg1	G03					1428:1430	G03	1428:1430	G03	1428:1430	The treated broilers (G03 and G04) also showed lower mortality percentage and E. coli recovery rates the liver and spleen than those in G02.
30187428	2	59	theme	E.	712:713	arg1	O78					720:722	E. coli O78	712:722	E. coli O78 at 28 days old	712:737	For this purpose, 101-day-old Cobb chicks were randomly allocated into four experimental groups (G)-G01: basal diet, G02: basal diet and challenged with E. coli O78 at 28 days old, G03: basal diet with probiotic mix and challenged with E. coli O78 at 28 days old, and G04: basal diet with IMO and challenged with E. coli O78 at 28 days old.
30187428	6	60	theme	total	1274:1278	arg1	lactobacilli-enterococci					1280:1303	total lactobacilli-enterococci	1274:1303	total lactobacilli-enterococci	1274:1303	The probiotic mix and IMO significantly increased the total lactobacilli and total lactobacilli-enterococci populations in the ceca of treated broilers, respectively compared with those in the control groups.
30187428	1	61	from	effects	231:237	arg1	protection					396:405	protection	396:405	protection against challenge with avian pathogenic Escherichia coli (APEC) O78	396:473	The current study conducted to investigate the effects of a multi-strain commercial probiotic mix and prebiotic (isomaltooligosaccharide, IMO) on broiler performance parameters, cecal microbiota composition, and protection against challenge with avian pathogenic Escherichia coli (APEC) O78.
30187428	1	61	from	effects	231:237	arg1	parameters					350:359	broiler performance parameters	330:359	broiler performance parameters	330:359	The current study conducted to investigate the effects of a multi-strain commercial probiotic mix and prebiotic (isomaltooligosaccharide, IMO) on broiler performance parameters, cecal microbiota composition, and protection against challenge with avian pathogenic Escherichia coli (APEC) O78.
30187428	1	61	from	effects	231:237	arg1	composition					379:389	cecal microbiota composition	362:389	cecal microbiota composition	362:389	The current study conducted to investigate the effects of a multi-strain commercial probiotic mix and prebiotic (isomaltooligosaccharide, IMO) on broiler performance parameters, cecal microbiota composition, and protection against challenge with avian pathogenic Escherichia coli (APEC) O78.
30187428	2	62	theme	G	573:573	arg1	groups					565:570	four experimental groups	547:570	four experimental groups (G)-G01: basal diet, G02: basal diet and challenged with E. coli O78 at 28 days old, G03: basal diet with probiotic mix and challenged with E. coli O78 at 28 days old, and G04: basal diet with IMO	547:767	For this purpose, 101-day-old Cobb chicks were randomly allocated into four experimental groups (G)-G01: basal diet, G02: basal diet and challenged with E. coli O78 at 28 days old, G03: basal diet with probiotic mix and challenged with E. coli O78 at 28 days old, and G04: basal diet with IMO and challenged with E. coli O78 at 28 days old.
30187428	2	63	theme	old	652:654	arg1	28 days					644:650	28 days	644:650	28 days old	644:654	For this purpose, 101-day-old Cobb chicks were randomly allocated into four experimental groups (G)-G01: basal diet, G02: basal diet and challenged with E. coli O78 at 28 days old, G03: basal diet with probiotic mix and challenged with E. coli O78 at 28 days old, and G04: basal diet with IMO and challenged with E. coli O78 at 28 days old.
30187428	2	63	theme	old	652:654	arg1	G03					657:659	G03	657:659	G03: basal diet	657:671	For this purpose, 101-day-old Cobb chicks were randomly allocated into four experimental groups (G)-G01: basal diet, G02: basal diet and challenged with E. coli O78 at 28 days old, G03: basal diet with probiotic mix and challenged with E. coli O78 at 28 days old, and G04: basal diet with IMO and challenged with E. coli O78 at 28 days old.
30187428	2	64	dep	E.	629:630	arg1	coli					632:635	coli	632:635	coli	632:635	For this purpose, 101-day-old Cobb chicks were randomly allocated into four experimental groups (G)-G01: basal diet, G02: basal diet and challenged with E. coli O78 at 28 days old, G03: basal diet with probiotic mix and challenged with E. coli O78 at 28 days old, and G04: basal diet with IMO and challenged with E. coli O78 at 28 days old.
30187428	1	65	dep	prebiotic	286:294	arg1	IMO					322:324	IMO	322:324	IMO	322:324	The current study conducted to investigate the effects of a multi-strain commercial probiotic mix and prebiotic (isomaltooligosaccharide, IMO) on broiler performance parameters, cecal microbiota composition, and protection against challenge with avian pathogenic Escherichia coli (APEC) O78.
30187428	1	65	dep	prebiotic	286:294	arg1	isomaltooligosaccharide					297:319	isomaltooligosaccharide	297:319	isomaltooligosaccharide	297:319	The current study conducted to investigate the effects of a multi-strain commercial probiotic mix and prebiotic (isomaltooligosaccharide, IMO) on broiler performance parameters, cecal microbiota composition, and protection against challenge with avian pathogenic Escherichia coli (APEC) O78.
30187428	2	66	theme	basal	662:666	arg1	diet					668:671	basal diet	662:671	G03: basal diet	657:671	For this purpose, 101-day-old Cobb chicks were randomly allocated into four experimental groups (G)-G01: basal diet, G02: basal diet and challenged with E. coli O78 at 28 days old, G03: basal diet with probiotic mix and challenged with E. coli O78 at 28 days old, and G04: basal diet with IMO and challenged with E. coli O78 at 28 days old.
30187428	5	67	theme	carcass	1056:1062	arg1	g					1072:1072	g	1072:1072	g	1072:1072	The hot carcass weight (g) was significantly higher in broiler chickens kept in G03 and G04 compared with those in the control groups (G01 and G02).
30187428	5	67	theme	carcass	1056:1062	arg1	weight					1064:1069	The hot carcass weight	1048:1069	The hot carcass weight (g)	1048:1073	The hot carcass weight (g) was significantly higher in broiler chickens kept in G03 and G04 compared with those in the control groups (G01 and G02).
30187428	5	67	theme	carcass	1056:1062	arg1	higher					1093:1098	higher	1093:1098	higher	1093:1098	The hot carcass weight (g) was significantly higher in broiler chickens kept in G03 and G04 compared with those in the control groups (G01 and G02).
30187428	1	68	theme	broiler	330:336	arg1	parameters					350:359	broiler performance parameters	330:359	broiler performance parameters	330:359	The current study conducted to investigate the effects of a multi-strain commercial probiotic mix and prebiotic (isomaltooligosaccharide, IMO) on broiler performance parameters, cecal microbiota composition, and protection against challenge with avian pathogenic Escherichia coli (APEC) O78.
30187428	3	69	theme	fourth	921:926	arg1	week					938:941	the fourth and fifth week	917:941	the fourth and fifth week	917:941	Results showed that weekly body weights in G03 were heavier (P < 0.05) than those of G01 and G02 at the fourth and fifth week.
30187428	6	70	theme	broilers	1340:1347	arg1	ceca					1324:1327	the ceca	1320:1327	the ceca of treated broilers	1320:1347	The probiotic mix and IMO significantly increased the total lactobacilli and total lactobacilli-enterococci populations in the ceca of treated broilers, respectively compared with those in the control groups.
30187428	2	71	dep	diet	604:607	arg1	challenged					613:622	challenged	613:622	challenged with E. coli O78 at 28 days old, G03: basal diet with probiotic mix	613:690	For this purpose, 101-day-old Cobb chicks were randomly allocated into four experimental groups (G)-G01: basal diet, G02: basal diet and challenged with E. coli O78 at 28 days old, G03: basal diet with probiotic mix and challenged with E. coli O78 at 28 days old, and G04: basal diet with IMO and challenged with E. coli O78 at 28 days old.
30187428	2	71	dep	diet	604:607	arg1	challenged					696:705	challenged	696:705	challenged with E. coli O78 at 28 days old, and G04	696:746	For this purpose, 101-day-old Cobb chicks were randomly allocated into four experimental groups (G)-G01: basal diet, G02: basal diet and challenged with E. coli O78 at 28 days old, G03: basal diet with probiotic mix and challenged with E. coli O78 at 28 days old, and G04: basal diet with IMO and challenged with E. coli O78 at 28 days old.
30187428	7	72	dep	rates	1501:1505	arg1	spleen					1521:1526	spleen	1521:1526	spleen	1521:1526	The treated broilers (G03 and G04) also showed lower mortality percentage and E. coli recovery rates the liver and spleen than those in G02.
30187428	7	72	dep	rates	1501:1505	arg1	liver					1511:1515	liver	1511:1515	liver	1511:1515	The treated broilers (G03 and G04) also showed lower mortality percentage and E. coli recovery rates the liver and spleen than those in G02.
30187428	7	72	dep	rates	1501:1505	arg1	rates					1501:1505	lower mortality percentage and E. coli recovery rates the liver and spleen	1453:1526	rates	1501:1505	The treated broilers (G03 and G04) also showed lower mortality percentage and E. coli recovery rates the liver and spleen than those in G02.
30187428	0	73	theme	Cecal	89:93	arg1	Composition					106:116	Cecal Microbiota Composition	89:116	Cecal Microbiota Composition	89:116	Effects of Dietary Supplementation of Probiotic Mix and Prebiotic on Growth Performance, Cecal Microbiota Composition, and Protection Against Escherichia coli O78 in Broiler Chickens.
30187428	6	74	theme	lactobacilli-enterococci	1280:1303	arg1	populations					1305:1315	the total lactobacilli and total lactobacilli-enterococci populations	1247:1315	the total lactobacilli and total lactobacilli-enterococci populations in the ceca of treated broilers	1247:1347	The probiotic mix and IMO significantly increased the total lactobacilli and total lactobacilli-enterococci populations in the ceca of treated broilers, respectively compared with those in the control groups.
30187428	1	75	theme	cecal	362:366	arg1	composition					379:389	cecal microbiota composition	362:389	cecal microbiota composition	362:389	The current study conducted to investigate the effects of a multi-strain commercial probiotic mix and prebiotic (isomaltooligosaccharide, IMO) on broiler performance parameters, cecal microbiota composition, and protection against challenge with avian pathogenic Escherichia coli (APEC) O78.
30187428	8	76	theme	probiotic	1569:1577	arg1	mix					1579:1581	probiotic mix	1569:1581	probiotic mix	1569:1581	It was concluded that probiotic mix or IMO significantly improved the growth performance and modulated the intestinal microbiota of broiler chickens challenged with APEC O78.
30187428	7	77	theme	percentage	1469:1478	arg1	spleen					1521:1526	spleen	1521:1526	spleen	1521:1526	The treated broilers (G03 and G04) also showed lower mortality percentage and E. coli recovery rates the liver and spleen than those in G02.
30187428	7	77	theme	percentage	1469:1478	arg1	liver					1511:1515	liver	1511:1515	liver	1511:1515	The treated broilers (G03 and G04) also showed lower mortality percentage and E. coli recovery rates the liver and spleen than those in G02.
30187428	7	77	theme	percentage	1469:1478	arg1	rates					1501:1505	lower mortality percentage and E. coli recovery rates the liver and spleen	1453:1526	rates	1501:1505	The treated broilers (G03 and G04) also showed lower mortality percentage and E. coli recovery rates the liver and spleen than those in G02.
30187428	2	78	theme	Cobb	506:509	arg1	chicks					511:516	101-day-old Cobb chicks	494:516	101-day-old Cobb chicks	494:516	For this purpose, 101-day-old Cobb chicks were randomly allocated into four experimental groups (G)-G01: basal diet, G02: basal diet and challenged with E. coli O78 at 28 days old, G03: basal diet with probiotic mix and challenged with E. coli O78 at 28 days old, and G04: basal diet with IMO and challenged with E. coli O78 at 28 days old.
30187428	3	79	theme	fifth	932:936	arg1	week					938:941	the fourth and fifth week	917:941	the fourth and fifth week	917:941	Results showed that weekly body weights in G03 were heavier (P < 0.05) than those of G01 and G02 at the fourth and fifth week.
30187428	7	80	theme	lower	1453:1457	arg1	spleen					1521:1526	spleen	1521:1526	spleen	1521:1526	The treated broilers (G03 and G04) also showed lower mortality percentage and E. coli recovery rates the liver and spleen than those in G02.
30187428	7	80	theme	lower	1453:1457	arg1	liver					1511:1515	liver	1511:1515	liver	1511:1515	The treated broilers (G03 and G04) also showed lower mortality percentage and E. coli recovery rates the liver and spleen than those in G02.
30187428	7	80	theme	lower	1453:1457	arg1	rates					1501:1505	lower mortality percentage and E. coli recovery rates the liver and spleen	1453:1526	rates	1501:1505	The treated broilers (G03 and G04) also showed lower mortality percentage and E. coli recovery rates the liver and spleen than those in G02.
30187428	2	81	theme	old	812:814	arg1	28 days					804:810	28 days	804:810	28 days old	804:814	For this purpose, 101-day-old Cobb chicks were randomly allocated into four experimental groups (G)-G01: basal diet, G02: basal diet and challenged with E. coli O78 at 28 days old, G03: basal diet with probiotic mix and challenged with E. coli O78 at 28 days old, and G04: basal diet with IMO and challenged with E. coli O78 at 28 days old.
30187428	4	82	theme	other	1034:1038	arg1	groups					1040:1045	the other groups	1030:1045	the other groups	1030:1045	The body gain at the fourth and fifth week was higher (P < 0.05) in G03 than those of the other groups.
30187428	0	83	theme	Supplementation	19:33	arg1	Effects					0:6	Effects	0:6	Effects of Dietary Supplementation of Probiotic Mix and Prebiotic on Growth Performance, Cecal Microbiota Composition, and Protection Against Escherichia coli O78 in Broiler Chickens.	0:182	Effects of Dietary Supplementation of Probiotic Mix and Prebiotic on Growth Performance, Cecal Microbiota Composition, and Protection Against Escherichia coli O78 in Broiler Chickens.
30187428	6	84	theme	control	1390:1396	arg1	groups					1398:1403	the control groups	1386:1403	the control groups	1386:1403	The probiotic mix and IMO significantly increased the total lactobacilli and total lactobacilli-enterococci populations in the ceca of treated broilers, respectively compared with those in the control groups.
30187428	0	85	theme	Probiotic	38:46	arg1	Mix					48:50	Probiotic Mix	38:50	Probiotic Mix	38:50	Effects of Dietary Supplementation of Probiotic Mix and Prebiotic on Growth Performance, Cecal Microbiota Composition, and Protection Against Escherichia coli O78 in Broiler Chickens.
30187428	3	86	from	weights	849:855	arg1	G03					860:862	G03	860:862	G03	860:862	Results showed that weekly body weights in G03 were heavier (P < 0.05) than those of G01 and G02 at the fourth and fifth week.
30187428	2	87	with	O78	637:639	arg1	mix					688:690	probiotic mix	678:690	probiotic mix	678:690	For this purpose, 101-day-old Cobb chicks were randomly allocated into four experimental groups (G)-G01: basal diet, G02: basal diet and challenged with E. coli O78 at 28 days old, G03: basal diet with probiotic mix and challenged with E. coli O78 at 28 days old, and G04: basal diet with IMO and challenged with E. coli O78 at 28 days old.
30187428	8	88	theme	chickens	1687:1694	arg1	microbiota					1665:1674	the intestinal microbiota	1650:1674	the intestinal microbiota of broiler chickens challenged with APEC O78	1650:1719	It was concluded that probiotic mix or IMO significantly improved the growth performance and modulated the intestinal microbiota of broiler chickens challenged with APEC O78.
30187428	3	89	theme	body	844:847	arg1	weights					849:855	weekly body weights	837:855	weekly body weights in G03	837:862	Results showed that weekly body weights in G03 were heavier (P < 0.05) than those of G01 and G02 at the fourth and fifth week.
30187428	2	90	theme	old	735:737	arg1	28 days					727:733	28 days	727:733	28 days old	727:737	For this purpose, 101-day-old Cobb chicks were randomly allocated into four experimental groups (G)-G01: basal diet, G02: basal diet and challenged with E. coli O78 at 28 days old, G03: basal diet with probiotic mix and challenged with E. coli O78 at 28 days old, and G04: basal diet with IMO and challenged with E. coli O78 at 28 days old.
30187428	6	91	theme	total	1251:1255	arg1	lactobacilli					1257:1268	total lactobacilli	1251:1268	total lactobacilli	1251:1268	The probiotic mix and IMO significantly increased the total lactobacilli and total lactobacilli-enterococci populations in the ceca of treated broilers, respectively compared with those in the control groups.
30187428	5	92	theme	hot	1052:1054	arg1	g					1072:1072	g	1072:1072	g	1072:1072	The hot carcass weight (g) was significantly higher in broiler chickens kept in G03 and G04 compared with those in the control groups (G01 and G02).
30187428	5	92	theme	hot	1052:1054	arg1	weight					1064:1069	The hot carcass weight	1048:1069	The hot carcass weight (g)	1048:1073	The hot carcass weight (g) was significantly higher in broiler chickens kept in G03 and G04 compared with those in the control groups (G01 and G02).
30187428	5	92	theme	hot	1052:1054	arg1	higher					1093:1098	higher	1093:1098	higher	1093:1098	The hot carcass weight (g) was significantly higher in broiler chickens kept in G03 and G04 compared with those in the control groups (G01 and G02).
30622588	2	0	theme	dose-dependent	498:511	arg1	manners					513:519	dose-dependent manners	498:519	dose-dependent manners	498:519	In in vitro assays, the En-MPS demonstrated stronger antioxidant activities in dose-dependent manners.
30622588	3	1	theme	lipid	757:761	arg1	metabolism					763:772	the lipid metabolism	753:772	the lipid metabolism	753:772	The mice experiments revealed that both En-MPS and MPS had potential effects on antioxidation, antiaging, and organ protection mainly by improving the antioxidant enzyme activities, decreasing the lipid peroxidation, and remitting the lipid metabolism.
30622588	0	2	theme	Agrocybe	99:106	arg1	aegerita					108:115	Agrocybe aegerita	99:115	Agrocybe aegerita	99:115	Polysaccharides with Antioxidative and Antiaging Activities from Enzymatic-Extractable Mycelium by Agrocybe aegerita (Brig.) Sing.
30622588	4	3	from	differences	910:920	arg1	activity					936:943	biological activity	925:943	biological activity of MPS and En-MPS samples	925:969	Furthermore, chemical composition and monosaccharide composition of polysaccharides were also measured, and the results indicated that differences in biological activity of MPS and En-MPS samples showed a significant correlation to their purity.
30622588	1	4	theme	antioxidant	167:177	arg1	effects					212:218	the antioxidant, antiaging, and organ protective effects	163:218	the antioxidant, antiaging, and organ protective effects of the water-extractable mycelium polysaccharides (MPS) and enzymic-extractable mycelium polysaccharides (En-MPS)	163:332	This study aimed to investigate the antioxidant, antiaging, and organ protective effects of the water-extractable mycelium polysaccharides (MPS) and enzymic-extractable mycelium polysaccharides (En-MPS) by Agrocybe aegerita (Brig.) Sing in D-galactose-induced (D-gal-induced) aging mice.
30622588	4	5	theme	monosaccharide	813:826	arg1	composition					828:838	monosaccharide composition	813:838	monosaccharide composition	813:838	Furthermore, chemical composition and monosaccharide composition of polysaccharides were also measured, and the results indicated that differences in biological activity of MPS and En-MPS samples showed a significant correlation to their purity.
30622588	4	6	theme	biological	925:934	arg1	activity					936:943	biological activity	925:943	biological activity of MPS and En-MPS samples	925:969	Furthermore, chemical composition and monosaccharide composition of polysaccharides were also measured, and the results indicated that differences in biological activity of MPS and En-MPS samples showed a significant correlation to their purity.
30622588	0	7	dep	Sing	125:128	arg1	Brig.					118:122	Brig.	118:122	Brig.	118:122	Polysaccharides with Antioxidative and Antiaging Activities from Enzymatic-Extractable Mycelium by Agrocybe aegerita (Brig.) Sing.
30622588	4	8	theme	significant	980:990	arg1	correlation					992:1002	a significant correlation	978:1002	a significant correlation to their purity	978:1018	Furthermore, chemical composition and monosaccharide composition of polysaccharides were also measured, and the results indicated that differences in biological activity of MPS and En-MPS samples showed a significant correlation to their purity.
30622588	5	9	theme	complications	1203:1215	arg1	prevention					1159:1168	prevention	1159:1168	prevention	1159:1168	The findings demonstrated that the polysaccharides by A. aegerita (Brig.) Sing could be exploited as natural and functional foods for the prevention and alleviation of aging and its complications.
30622588	5	9	theme	complications	1203:1215	arg1	alleviation					1174:1184	alleviation	1174:1184	alleviation	1174:1184	The findings demonstrated that the polysaccharides by A. aegerita (Brig.) Sing could be exploited as natural and functional foods for the prevention and alleviation of aging and its complications.
30622588	5	10	theme	functional	1134:1143	arg1	foods					1145:1149	natural and functional foods	1122:1149	natural and functional foods for the prevention and alleviation of aging and its complications	1122:1215	The findings demonstrated that the polysaccharides by A. aegerita (Brig.) Sing could be exploited as natural and functional foods for the prevention and alleviation of aging and its complications.
30622588	5	10	theme	functional	1134:1143	arg1	polysaccharides					1056:1070	the polysaccharides	1052:1070	the polysaccharides by A. aegerita (Brig.) Sing	1052:1098	The findings demonstrated that the polysaccharides by A. aegerita (Brig.) Sing could be exploited as natural and functional foods for the prevention and alleviation of aging and its complications.
30622588	2	11	theme	in	422:423	arg1	assays					431:436	in vitro assays	422:436	in vitro assays	422:436	In in vitro assays, the En-MPS demonstrated stronger antioxidant activities in dose-dependent manners.
30622588	5	12	theme	natural	1122:1128	arg1	foods					1145:1149	natural and functional foods	1122:1149	natural and functional foods for the prevention and alleviation of aging and its complications	1122:1215	The findings demonstrated that the polysaccharides by A. aegerita (Brig.) Sing could be exploited as natural and functional foods for the prevention and alleviation of aging and its complications.
30622588	5	12	theme	natural	1122:1128	arg1	polysaccharides					1056:1070	the polysaccharides	1052:1070	the polysaccharides by A. aegerita (Brig.) Sing	1052:1098	The findings demonstrated that the polysaccharides by A. aegerita (Brig.) Sing could be exploited as natural and functional foods for the prevention and alleviation of aging and its complications.
30622588	1	13	theme	D-galactose-induced	371:389	arg1	mice					413:416	D-galactose-induced (D-gal-induced) aging mice	371:416	D-galactose-induced (D-gal-induced) aging mice	371:416	This study aimed to investigate the antioxidant, antiaging, and organ protective effects of the water-extractable mycelium polysaccharides (MPS) and enzymic-extractable mycelium polysaccharides (En-MPS) by Agrocybe aegerita (Brig.) Sing in D-galactose-induced (D-gal-induced) aging mice.
30622588	1	14	theme	antiaging	180:188	arg1	effects					212:218	the antioxidant, antiaging, and organ protective effects	163:218	the antioxidant, antiaging, and organ protective effects of the water-extractable mycelium polysaccharides (MPS) and enzymic-extractable mycelium polysaccharides (En-MPS)	163:332	This study aimed to investigate the antioxidant, antiaging, and organ protective effects of the water-extractable mycelium polysaccharides (MPS) and enzymic-extractable mycelium polysaccharides (En-MPS) by Agrocybe aegerita (Brig.) Sing in D-galactose-induced (D-gal-induced) aging mice.
30622588	3	15	contain	had	577:579	arg1	En-MPS					562:567	En-MPS	562:567	En-MPS	562:567	The mice experiments revealed that both En-MPS and MPS had potential effects on antioxidation, antiaging, and organ protection mainly by improving the antioxidant enzyme activities, decreasing the lipid peroxidation, and remitting the lipid metabolism.
30622588	3	15	contain	had	577:579	arg2	effects					591:597	potential effects	581:597	potential effects on antioxidation, antiaging, and organ protection	581:647	The mice experiments revealed that both En-MPS and MPS had potential effects on antioxidation, antiaging, and organ protection mainly by improving the antioxidant enzyme activities, decreasing the lipid peroxidation, and remitting the lipid metabolism.
30622588	3	15	contain	had	577:579	arg1	MPS					573:575	MPS	573:575	MPS	573:575	The mice experiments revealed that both En-MPS and MPS had potential effects on antioxidation, antiaging, and organ protection mainly by improving the antioxidant enzyme activities, decreasing the lipid peroxidation, and remitting the lipid metabolism.
30622588	3	16	theme	potential	581:589	arg1	effects					591:597	potential effects	581:597	potential effects on antioxidation, antiaging, and organ protection	581:647	The mice experiments revealed that both En-MPS and MPS had potential effects on antioxidation, antiaging, and organ protection mainly by improving the antioxidant enzyme activities, decreasing the lipid peroxidation, and remitting the lipid metabolism.
30622588	1	17	theme	enzymic-extractable	280:298	arg1	polysaccharides					309:323	enzymic-extractable mycelium polysaccharides	280:323	enzymic-extractable mycelium polysaccharides (En-MPS)	280:332	This study aimed to investigate the antioxidant, antiaging, and organ protective effects of the water-extractable mycelium polysaccharides (MPS) and enzymic-extractable mycelium polysaccharides (En-MPS) by Agrocybe aegerita (Brig.) Sing in D-galactose-induced (D-gal-induced) aging mice.
30622588	1	17	theme	enzymic-extractable	280:298	arg1	En-MPS					326:331	En-MPS	326:331	En-MPS	326:331	This study aimed to investigate the antioxidant, antiaging, and organ protective effects of the water-extractable mycelium polysaccharides (MPS) and enzymic-extractable mycelium polysaccharides (En-MPS) by Agrocybe aegerita (Brig.) Sing in D-galactose-induced (D-gal-induced) aging mice.
30622588	4	18	theme	chemical	788:795	arg1	composition					797:807	chemical composition	788:807	chemical composition	788:807	Furthermore, chemical composition and monosaccharide composition of polysaccharides were also measured, and the results indicated that differences in biological activity of MPS and En-MPS samples showed a significant correlation to their purity.
30622588	1	19	theme	mycelium	300:307	arg1	polysaccharides					309:323	enzymic-extractable mycelium polysaccharides	280:323	enzymic-extractable mycelium polysaccharides (En-MPS)	280:332	This study aimed to investigate the antioxidant, antiaging, and organ protective effects of the water-extractable mycelium polysaccharides (MPS) and enzymic-extractable mycelium polysaccharides (En-MPS) by Agrocybe aegerita (Brig.) Sing in D-galactose-induced (D-gal-induced) aging mice.
30622588	1	19	theme	mycelium	300:307	arg1	En-MPS					326:331	En-MPS	326:331	En-MPS	326:331	This study aimed to investigate the antioxidant, antiaging, and organ protective effects of the water-extractable mycelium polysaccharides (MPS) and enzymic-extractable mycelium polysaccharides (En-MPS) by Agrocybe aegerita (Brig.) Sing in D-galactose-induced (D-gal-induced) aging mice.
30622588	4	20	theme	samples	963:969	arg1	activity					936:943	biological activity	925:943	biological activity of MPS and En-MPS samples	925:969	Furthermore, chemical composition and monosaccharide composition of polysaccharides were also measured, and the results indicated that differences in biological activity of MPS and En-MPS samples showed a significant correlation to their purity.
30622588	1	21	theme	organ	195:199	arg1	effects					212:218	the antioxidant, antiaging, and organ protective effects	163:218	the antioxidant, antiaging, and organ protective effects of the water-extractable mycelium polysaccharides (MPS) and enzymic-extractable mycelium polysaccharides (En-MPS)	163:332	This study aimed to investigate the antioxidant, antiaging, and organ protective effects of the water-extractable mycelium polysaccharides (MPS) and enzymic-extractable mycelium polysaccharides (En-MPS) by Agrocybe aegerita (Brig.) Sing in D-galactose-induced (D-gal-induced) aging mice.
30622588	1	22	theme	polysaccharides	309:323	arg1	effects					212:218	the antioxidant, antiaging, and organ protective effects	163:218	the antioxidant, antiaging, and organ protective effects of the water-extractable mycelium polysaccharides (MPS) and enzymic-extractable mycelium polysaccharides (En-MPS)	163:332	This study aimed to investigate the antioxidant, antiaging, and organ protective effects of the water-extractable mycelium polysaccharides (MPS) and enzymic-extractable mycelium polysaccharides (En-MPS) by Agrocybe aegerita (Brig.) Sing in D-galactose-induced (D-gal-induced) aging mice.
30622588	4	23	theme	En-MPS	956:961	arg1	samples					963:969	MPS and En-MPS samples	948:969	MPS and En-MPS samples	948:969	Furthermore, chemical composition and monosaccharide composition of polysaccharides were also measured, and the results indicated that differences in biological activity of MPS and En-MPS samples showed a significant correlation to their purity.
30622588	3	24	theme	mice	526:529	arg1	experiments					531:541	The mice experiments	522:541	The mice experiments	522:541	The mice experiments revealed that both En-MPS and MPS had potential effects on antioxidation, antiaging, and organ protection mainly by improving the antioxidant enzyme activities, decreasing the lipid peroxidation, and remitting the lipid metabolism.
30622588	1	25	theme	protective	201:210	arg1	effects					212:218	the antioxidant, antiaging, and organ protective effects	163:218	the antioxidant, antiaging, and organ protective effects of the water-extractable mycelium polysaccharides (MPS) and enzymic-extractable mycelium polysaccharides (En-MPS)	163:332	This study aimed to investigate the antioxidant, antiaging, and organ protective effects of the water-extractable mycelium polysaccharides (MPS) and enzymic-extractable mycelium polysaccharides (En-MPS) by Agrocybe aegerita (Brig.) Sing in D-galactose-induced (D-gal-induced) aging mice.
30622588	5	26	dep	prevention	1159:1168	arg1	the					1155:1157	the	1155:1157	the	1155:1157	The findings demonstrated that the polysaccharides by A. aegerita (Brig.) Sing could be exploited as natural and functional foods for the prevention and alleviation of aging and its complications.
30622588	2	27	theme	antioxidant	472:482	arg1	activities					484:493	stronger antioxidant activities	463:493	stronger antioxidant activities	463:493	In in vitro assays, the En-MPS demonstrated stronger antioxidant activities in dose-dependent manners.
30622588	1	28	dep	D-galactose-induced	371:389	arg1	D-gal-induced					392:404	D-gal-induced	392:404	D-gal-induced	392:404	This study aimed to investigate the antioxidant, antiaging, and organ protective effects of the water-extractable mycelium polysaccharides (MPS) and enzymic-extractable mycelium polysaccharides (En-MPS) by Agrocybe aegerita (Brig.) Sing in D-galactose-induced (D-gal-induced) aging mice.
30622588	1	29	theme	aging	407:411	arg1	mice					413:416	D-galactose-induced (D-gal-induced) aging mice	371:416	D-galactose-induced (D-gal-induced) aging mice	371:416	This study aimed to investigate the antioxidant, antiaging, and organ protective effects of the water-extractable mycelium polysaccharides (MPS) and enzymic-extractable mycelium polysaccharides (En-MPS) by Agrocybe aegerita (Brig.) Sing in D-galactose-induced (D-gal-induced) aging mice.
30622588	5	30	theme	A.	1075:1076	arg1	Brig.					1088:1092	Brig.	1088:1092	Brig.	1088:1092	The findings demonstrated that the polysaccharides by A. aegerita (Brig.) Sing could be exploited as natural and functional foods for the prevention and alleviation of aging and its complications.
30622588	5	30	theme	A.	1075:1076	arg1	aegerita					1078:1085	A. aegerita	1075:1085	A. aegerita (Brig.) Sing	1075:1098	The findings demonstrated that the polysaccharides by A. aegerita (Brig.) Sing could be exploited as natural and functional foods for the prevention and alleviation of aging and its complications.
30622588	0	31	theme	Enzymatic-Extractable	65:85	arg1	Mycelium					87:94	Enzymatic-Extractable Mycelium	65:94	Enzymatic-Extractable Mycelium by Agrocybe aegerita	65:115	Polysaccharides with Antioxidative and Antiaging Activities from Enzymatic-Extractable Mycelium by Agrocybe aegerita (Brig.) Sing.
30622588	5	32	theme	aging	1189:1193	arg1	prevention					1159:1168	prevention	1159:1168	prevention	1159:1168	The findings demonstrated that the polysaccharides by A. aegerita (Brig.) Sing could be exploited as natural and functional foods for the prevention and alleviation of aging and its complications.
30622588	5	32	theme	aging	1189:1193	arg1	alleviation					1174:1184	alleviation	1174:1184	alleviation	1174:1184	The findings demonstrated that the polysaccharides by A. aegerita (Brig.) Sing could be exploited as natural and functional foods for the prevention and alleviation of aging and its complications.
30622588	0	33	from	Mycelium	87:94	arg1	Polysaccharides					0:14	Polysaccharides	0:14	Polysaccharides with Antioxidative and Antiaging Activities from Enzymatic-Extractable Mycelium by Agrocybe aegerita	0:115	Polysaccharides with Antioxidative and Antiaging Activities from Enzymatic-Extractable Mycelium by Agrocybe aegerita (Brig.) Sing.
30622588	2	34	dep	in	422:423	arg1	vitro					425:429	vitro	425:429	vitro	425:429	In in vitro assays, the En-MPS demonstrated stronger antioxidant activities in dose-dependent manners.
30622588	1	35	theme	Agrocybe	337:344	arg1	Brig.					356:360	Brig.	356:360	Brig.	356:360	This study aimed to investigate the antioxidant, antiaging, and organ protective effects of the water-extractable mycelium polysaccharides (MPS) and enzymic-extractable mycelium polysaccharides (En-MPS) by Agrocybe aegerita (Brig.) Sing in D-galactose-induced (D-gal-induced) aging mice.
30622588	1	35	theme	Agrocybe	337:344	arg1	aegerita					346:353	Agrocybe aegerita	337:353	Agrocybe aegerita (Brig.) Sing in D-galactose-induced (D-gal-induced) aging mice	337:416	This study aimed to investigate the antioxidant, antiaging, and organ protective effects of the water-extractable mycelium polysaccharides (MPS) and enzymic-extractable mycelium polysaccharides (En-MPS) by Agrocybe aegerita (Brig.) Sing in D-galactose-induced (D-gal-induced) aging mice.
30622588	3	36	theme	organ	632:636	arg1	protection					638:647	organ protection	632:647	organ protection	632:647	The mice experiments revealed that both En-MPS and MPS had potential effects on antioxidation, antiaging, and organ protection mainly by improving the antioxidant enzyme activities, decreasing the lipid peroxidation, and remitting the lipid metabolism.
30622588	0	37	with	Polysaccharides	0:14	arg1	Antioxidative					21:33	Antioxidative	21:33	Antioxidative	21:33	Polysaccharides with Antioxidative and Antiaging Activities from Enzymatic-Extractable Mycelium by Agrocybe aegerita (Brig.) Sing.
30622588	0	37	with	Polysaccharides	0:14	arg1	Antiaging					39:47	Antiaging	39:47	Antiaging	39:47	Polysaccharides with Antioxidative and Antiaging Activities from Enzymatic-Extractable Mycelium by Agrocybe aegerita (Brig.) Sing.
30622588	4	38	theme	MPS	948:950	arg1	samples					963:969	MPS and En-MPS samples	948:969	MPS and En-MPS samples	948:969	Furthermore, chemical composition and monosaccharide composition of polysaccharides were also measured, and the results indicated that differences in biological activity of MPS and En-MPS samples showed a significant correlation to their purity.
30622588	3	39	theme	antioxidant	673:683	arg1	activities					692:701	the antioxidant enzyme activities	669:701	the antioxidant enzyme activities	669:701	The mice experiments revealed that both En-MPS and MPS had potential effects on antioxidation, antiaging, and organ protection mainly by improving the antioxidant enzyme activities, decreasing the lipid peroxidation, and remitting the lipid metabolism.
30622588	0	40	dep	Antioxidative	21:33	arg1	Activities					49:58	Activities	49:58	Activities	49:58	Polysaccharides with Antioxidative and Antiaging Activities from Enzymatic-Extractable Mycelium by Agrocybe aegerita (Brig.) Sing.
30622588	1	41	theme	water-extractable	227:243	arg1	MPS					271:273	MPS	271:273	MPS	271:273	This study aimed to investigate the antioxidant, antiaging, and organ protective effects of the water-extractable mycelium polysaccharides (MPS) and enzymic-extractable mycelium polysaccharides (En-MPS) by Agrocybe aegerita (Brig.) Sing in D-galactose-induced (D-gal-induced) aging mice.
30622588	1	41	theme	water-extractable	227:243	arg1	polysaccharides					254:268	the water-extractable mycelium polysaccharides	223:268	the water-extractable mycelium polysaccharides (MPS)	223:274	This study aimed to investigate the antioxidant, antiaging, and organ protective effects of the water-extractable mycelium polysaccharides (MPS) and enzymic-extractable mycelium polysaccharides (En-MPS) by Agrocybe aegerita (Brig.) Sing in D-galactose-induced (D-gal-induced) aging mice.
30622588	3	42	theme	enzyme	685:690	arg1	activities					692:701	the antioxidant enzyme activities	669:701	the antioxidant enzyme activities	669:701	The mice experiments revealed that both En-MPS and MPS had potential effects on antioxidation, antiaging, and organ protection mainly by improving the antioxidant enzyme activities, decreasing the lipid peroxidation, and remitting the lipid metabolism.
30622588	4	43	theme	polysaccharides	843:857	arg1	composition					797:807	chemical composition	788:807	chemical composition	788:807	Furthermore, chemical composition and monosaccharide composition of polysaccharides were also measured, and the results indicated that differences in biological activity of MPS and En-MPS samples showed a significant correlation to their purity.
30622588	4	43	theme	polysaccharides	843:857	arg1	composition					828:838	monosaccharide composition	813:838	monosaccharide composition	813:838	Furthermore, chemical composition and monosaccharide composition of polysaccharides were also measured, and the results indicated that differences in biological activity of MPS and En-MPS samples showed a significant correlation to their purity.
30622588	3	44	from	effects	591:597	arg1	antiaging					617:625	antiaging	617:625	antiaging	617:625	The mice experiments revealed that both En-MPS and MPS had potential effects on antioxidation, antiaging, and organ protection mainly by improving the antioxidant enzyme activities, decreasing the lipid peroxidation, and remitting the lipid metabolism.
30622588	3	44	from	effects	591:597	arg1	antioxidation					602:614	antioxidation	602:614	antioxidation	602:614	The mice experiments revealed that both En-MPS and MPS had potential effects on antioxidation, antiaging, and organ protection mainly by improving the antioxidant enzyme activities, decreasing the lipid peroxidation, and remitting the lipid metabolism.
30622588	3	44	from	effects	591:597	arg1	protection					638:647	organ protection	632:647	organ protection	632:647	The mice experiments revealed that both En-MPS and MPS had potential effects on antioxidation, antiaging, and organ protection mainly by improving the antioxidant enzyme activities, decreasing the lipid peroxidation, and remitting the lipid metabolism.
30622588	1	45	theme	mycelium	245:252	arg1	MPS					271:273	MPS	271:273	MPS	271:273	This study aimed to investigate the antioxidant, antiaging, and organ protective effects of the water-extractable mycelium polysaccharides (MPS) and enzymic-extractable mycelium polysaccharides (En-MPS) by Agrocybe aegerita (Brig.) Sing in D-galactose-induced (D-gal-induced) aging mice.
30622588	1	45	theme	mycelium	245:252	arg1	polysaccharides					254:268	the water-extractable mycelium polysaccharides	223:268	the water-extractable mycelium polysaccharides (MPS)	223:274	This study aimed to investigate the antioxidant, antiaging, and organ protective effects of the water-extractable mycelium polysaccharides (MPS) and enzymic-extractable mycelium polysaccharides (En-MPS) by Agrocybe aegerita (Brig.) Sing in D-galactose-induced (D-gal-induced) aging mice.
30622588	2	46	theme	stronger	463:470	arg1	activities					484:493	stronger antioxidant activities	463:493	stronger antioxidant activities	463:493	In in vitro assays, the En-MPS demonstrated stronger antioxidant activities in dose-dependent manners.
30622588	3	47	theme	lipid	719:723	arg1	peroxidation					725:736	the lipid peroxidation	715:736	the lipid peroxidation	715:736	The mice experiments revealed that both En-MPS and MPS had potential effects on antioxidation, antiaging, and organ protection mainly by improving the antioxidant enzyme activities, decreasing the lipid peroxidation, and remitting the lipid metabolism.
30622588	1	48	theme	polysaccharides	254:268	arg1	effects					212:218	the antioxidant, antiaging, and organ protective effects	163:218	the antioxidant, antiaging, and organ protective effects of the water-extractable mycelium polysaccharides (MPS) and enzymic-extractable mycelium polysaccharides (En-MPS)	163:332	This study aimed to investigate the antioxidant, antiaging, and organ protective effects of the water-extractable mycelium polysaccharides (MPS) and enzymic-extractable mycelium polysaccharides (En-MPS) by Agrocybe aegerita (Brig.) Sing in D-galactose-induced (D-gal-induced) aging mice.
31310199	12	0	theme	LMG	1579:1581	arg1	30035T					1583:1588	strain LMG 30035T	1572:1588	strain LMG 30035T	1572:1588	In summary, these characteristics distinguish strain LMG 30035T from validly named species of the genus Actinomadura, and therefore, we propose to classify this strain formally as the novel species Actinomadura roseirufa sp.
31310199	6	1	theme	meso-diaminopimelic	1112:1130	arg1	acid					1132:1135	meso-diaminopimelic acid	1112:1135	meso-diaminopimelic acid	1112:1135	Analysis of the cell wall revealed the presence of meso-diaminopimelic acid in the peptidoglycan.
31310199	4	2	theme	16S	576:578	arg1	rRNA					580:583	nearly complete 16S rRNA	560:583	nearly complete 16S rRNA gene sequence	560:597	Based on analysis of nearly complete 16S rRNA gene sequence, strain LMG 30035T was found to be closely related to the type strains of Actinomadura fibrosa ATCC 49459T (98.88 %) and Actinomadura formosensis JCM 7474T (98.82 %) (pairwise similarity values in parentheses).
31310199	13	3	theme	=CECT	1773:1777	arg1	53664T					1791:1796	=ATCC 53664T	1785:1796	=ATCC 53664T	1785:1796	nov. with LMG 30035T (=CECT 9808T,=ATCC 53664T) as the type strain.
31310199	13	3	theme	=CECT	1773:1777	arg1	9808T					1779:1783	=CECT 9808T	1773:1783	=CECT 9808T	1773:1783	nov. with LMG 30035T (=CECT 9808T,=ATCC 53664T) as the type strain.
31310199	2	4	theme	Yamae	251:255	arg1	Village					257:263	Yamae Village	251:263	Yamae Village	251:263	The taxonomic position of 'Actinomadura roseorufa' LMG 30035T, a semduramicin-producing mutant of strain ATCC 53666P, which was isolated from a soil sample collected in Yamae Village, Kamamoto, Japan, was clarified in the present study using a polyphasic approach.
31310199	4	5	theme	similarity	775:784	arg1	values					786:791	pairwise similarity values	766:791	pairwise similarity values in parentheses	766:806	Based on analysis of nearly complete 16S rRNA gene sequence, strain LMG 30035T was found to be closely related to the type strains of Actinomadura fibrosa ATCC 49459T (98.88 %) and Actinomadura formosensis JCM 7474T (98.82 %) (pairwise similarity values in parentheses).
31310199	11	6	theme	DNA	1481:1483	arg1	G+C content					1485:1495	The DNA G+C content	1477:1495	The DNA G+C content of its genome	1477:1509	The DNA G+C content of its genome was 72.5 mol%.
31310199	11	6	theme	DNA	1481:1483	arg1	%					1523:1523	72.5 mol%	1515:1523	72.5 mol%	1515:1523	The DNA G+C content of its genome was 72.5 mol%.
31310199	4	7	dep	7474T	749:753	arg1	values					786:791	pairwise similarity values	766:791	pairwise similarity values in parentheses	766:806	Based on analysis of nearly complete 16S rRNA gene sequence, strain LMG 30035T was found to be closely related to the type strains of Actinomadura fibrosa ATCC 49459T (98.88 %) and Actinomadura formosensis JCM 7474T (98.82 %) (pairwise similarity values in parentheses).
31310199	5	8	dep	%	971:971	arg1	83.1 					966:970	83.1 	966:970	83.1 	966:970	Digital DNA-DNA hybridisation experiments revealed unambiguously that strain LMG 30035T represents a novel Actinomadura species (OrthoANIu values less than 83.1 %; dDDH values less than 27.2 % with type strains of validly named Actinomadura species).
31310199	5	9	theme	Digital	810:816	arg1	experiments					840:850	Digital DNA-DNA hybridisation experiments	810:850	Digital DNA-DNA hybridisation experiments	810:850	Digital DNA-DNA hybridisation experiments revealed unambiguously that strain LMG 30035T represents a novel Actinomadura species (OrthoANIu values less than 83.1 %; dDDH values less than 27.2 % with type strains of validly named Actinomadura species).
31310199	6	10	theme	cell	1077:1080	arg1	wall					1082:1085	the cell wall	1073:1085	the cell wall	1073:1085	Analysis of the cell wall revealed the presence of meso-diaminopimelic acid in the peptidoglycan.
31310199	4	11	theme	LMG	607:609	arg1	30035T					611:616	strain LMG 30035T	600:616	strain LMG 30035T	600:616	Based on analysis of nearly complete 16S rRNA gene sequence, strain LMG 30035T was found to be closely related to the type strains of Actinomadura fibrosa ATCC 49459T (98.88 %) and Actinomadura formosensis JCM 7474T (98.82 %) (pairwise similarity values in parentheses).
31310199	5	12	dep	values	949:954	arg1	%					971:971	%	971:971	%	971:971	Digital DNA-DNA hybridisation experiments revealed unambiguously that strain LMG 30035T represents a novel Actinomadura species (OrthoANIu values less than 83.1 %; dDDH values less than 27.2 % with type strains of validly named Actinomadura species).
31310199	5	12	dep	values	949:954	arg1	values					979:984	dDDH values	974:984	dDDH values less than 27.2 % with type strains of validly named Actinomadura species	974:1057	Digital DNA-DNA hybridisation experiments revealed unambiguously that strain LMG 30035T represents a novel Actinomadura species (OrthoANIu values less than 83.1 %; dDDH values less than 27.2 % with type strains of validly named Actinomadura species).
31310199	2	13	theme	taxonomic	86:94	arg1	position					96:103	The taxonomic position	82:103	The taxonomic position of 'Actinomadura roseorufa' LMG 30035T, a semduramicin-producing mutant of strain ATCC 53666P, which was isolated from a soil sample collected in Yamae Village, Kamamoto, Japan,	82:281	The taxonomic position of 'Actinomadura roseorufa' LMG 30035T, a semduramicin-producing mutant of strain ATCC 53666P, which was isolated from a soil sample collected in Yamae Village, Kamamoto, Japan, was clarified in the present study using a polyphasic approach.
31310199	5	14	theme	less	986:989	arg1	values					979:984	dDDH values	974:984	dDDH values less than 27.2 % with type strains of validly named Actinomadura species	974:1057	Digital DNA-DNA hybridisation experiments revealed unambiguously that strain LMG 30035T represents a novel Actinomadura species (OrthoANIu values less than 83.1 %; dDDH values less than 27.2 % with type strains of validly named Actinomadura species).
31310199	4	15	theme	fibrosa	686:692	arg1	49459T					699:704	Actinomadura fibrosa ATCC 49459T	673:704	Actinomadura fibrosa ATCC 49459T (98.88 %)	673:714	Based on analysis of nearly complete 16S rRNA gene sequence, strain LMG 30035T was found to be closely related to the type strains of Actinomadura fibrosa ATCC 49459T (98.88 %) and Actinomadura formosensis JCM 7474T (98.82 %) (pairwise similarity values in parentheses).
31310199	4	15	theme	fibrosa	686:692	arg1	%					713:713	98.88 %	707:713	98.88 %	707:713	Based on analysis of nearly complete 16S rRNA gene sequence, strain LMG 30035T was found to be closely related to the type strains of Actinomadura fibrosa ATCC 49459T (98.88 %) and Actinomadura formosensis JCM 7474T (98.82 %) (pairwise similarity values in parentheses).
31310199	2	16	theme	soil	226:229	arg1	sample					231:236	a soil sample	224:236	a soil sample collected in Yamae Village	224:263	The taxonomic position of 'Actinomadura roseorufa' LMG 30035T, a semduramicin-producing mutant of strain ATCC 53666P, which was isolated from a soil sample collected in Yamae Village, Kamamoto, Japan, was clarified in the present study using a polyphasic approach.
31310199	5	17	theme	LMG	887:889	arg1	30035T					891:896	strain LMG 30035T	880:896	strain LMG 30035T	880:896	Digital DNA-DNA hybridisation experiments revealed unambiguously that strain LMG 30035T represents a novel Actinomadura species (OrthoANIu values less than 83.1 %; dDDH values less than 27.2 % with type strains of validly named Actinomadura species).
31310199	13	18	dep	30035T	1765:1770	arg1	53664T					1791:1796	=ATCC 53664T	1785:1796	=ATCC 53664T	1785:1796	nov. with LMG 30035T (=CECT 9808T,=ATCC 53664T) as the type strain.
31310199	13	18	dep	30035T	1765:1770	arg1	9808T					1779:1783	=CECT 9808T	1773:1783	=CECT 9808T	1773:1783	nov. with LMG 30035T (=CECT 9808T,=ATCC 53664T) as the type strain.
31310199	8	19	theme	polar	1247:1251	arg1	lipids					1253:1258	The major polar lipids	1237:1258	The major polar lipids	1237:1258	The major polar lipids included phosphatidylinositol and diphosphatidylglycerol.
31310199	5	20	theme	hybridisation	826:838	arg1	experiments					840:850	Digital DNA-DNA hybridisation experiments	810:850	Digital DNA-DNA hybridisation experiments	810:850	Digital DNA-DNA hybridisation experiments revealed unambiguously that strain LMG 30035T represents a novel Actinomadura species (OrthoANIu values less than 83.1 %; dDDH values less than 27.2 % with type strains of validly named Actinomadura species).
31310199	4	21	theme	type	657:660	arg1	strains					662:668	the type strains	653:668	the type strains of Actinomadura fibrosa ATCC 49459T (98.88 %) and Actinomadura formosensis JCM 7474T (98.82 %) (pairwise similarity values in parentheses)	653:807	Based on analysis of nearly complete 16S rRNA gene sequence, strain LMG 30035T was found to be closely related to the type strains of Actinomadura fibrosa ATCC 49459T (98.88 %) and Actinomadura formosensis JCM 7474T (98.82 %) (pairwise similarity values in parentheses).
31310199	12	22	theme	species	1716:1722	arg1	strain					1687:1692	this strain	1682:1692	this strain	1682:1692	In summary, these characteristics distinguish strain LMG 30035T from validly named species of the genus Actinomadura, and therefore, we propose to classify this strain formally as the novel species Actinomadura roseirufa sp.
31310199	12	22	theme	species	1716:1722	arg1	sp					1747:1748	the novel species Actinomadura roseirufa sp	1706:1748	the novel species Actinomadura roseirufa sp	1706:1748	In summary, these characteristics distinguish strain LMG 30035T from validly named species of the genus Actinomadura, and therefore, we propose to classify this strain formally as the novel species Actinomadura roseirufa sp.
31310199	2	23	attach	isolated	210:217	arg2	ATCC					187:190	strain ATCC 53666P	180:197	strain ATCC 53666P	180:197	The taxonomic position of 'Actinomadura roseorufa' LMG 30035T, a semduramicin-producing mutant of strain ATCC 53666P, which was isolated from a soil sample collected in Yamae Village, Kamamoto, Japan, was clarified in the present study using a polyphasic approach.
31310199	2	23	attach	isolated	210:217	arg1	sample					231:236	a soil sample	224:236	a soil sample collected in Yamae Village	224:263	The taxonomic position of 'Actinomadura roseorufa' LMG 30035T, a semduramicin-producing mutant of strain ATCC 53666P, which was isolated from a soil sample collected in Yamae Village, Kamamoto, Japan, was clarified in the present study using a polyphasic approach.
31310199	12	24	theme	roseirufa	1737:1745	arg1	strain					1687:1692	this strain	1682:1692	this strain	1682:1692	In summary, these characteristics distinguish strain LMG 30035T from validly named species of the genus Actinomadura, and therefore, we propose to classify this strain formally as the novel species Actinomadura roseirufa sp.
31310199	12	24	theme	roseirufa	1737:1745	arg1	sp					1747:1748	the novel species Actinomadura roseirufa sp	1706:1748	the novel species Actinomadura roseirufa sp	1706:1748	In summary, these characteristics distinguish strain LMG 30035T from validly named species of the genus Actinomadura, and therefore, we propose to classify this strain formally as the novel species Actinomadura roseirufa sp.
31310199	3	25	dep	substrate	451:459	arg1	a					395:395	a	395:395	a	395:395	This Gram-positive, aerobic actinomycete formed a well-developed, extensively branched, non-fragmenting substrate and aerial mycelia which differentiated into single, smooth-appearing spores.
31310199	4	26	theme	Actinomadura	673:684	arg1	49459T					699:704	Actinomadura fibrosa ATCC 49459T	673:704	Actinomadura fibrosa ATCC 49459T (98.88 %)	673:714	Based on analysis of nearly complete 16S rRNA gene sequence, strain LMG 30035T was found to be closely related to the type strains of Actinomadura fibrosa ATCC 49459T (98.88 %) and Actinomadura formosensis JCM 7474T (98.82 %) (pairwise similarity values in parentheses).
31310199	4	26	theme	Actinomadura	673:684	arg1	%					713:713	98.88 %	707:713	98.88 %	707:713	Based on analysis of nearly complete 16S rRNA gene sequence, strain LMG 30035T was found to be closely related to the type strains of Actinomadura fibrosa ATCC 49459T (98.88 %) and Actinomadura formosensis JCM 7474T (98.82 %) (pairwise similarity values in parentheses).
31310199	4	27	theme	Actinomadura	720:731	arg1	7474T					749:753	Actinomadura formosensis JCM 7474T	720:753	Actinomadura formosensis JCM 7474T (98.82 %) (pairwise similarity values in parentheses)	720:807	Based on analysis of nearly complete 16S rRNA gene sequence, strain LMG 30035T was found to be closely related to the type strains of Actinomadura fibrosa ATCC 49459T (98.88 %) and Actinomadura formosensis JCM 7474T (98.82 %) (pairwise similarity values in parentheses).
31310199	4	27	theme	Actinomadura	720:731	arg1	%					762:762	98.82 %	756:762	98.82 %	756:762	Based on analysis of nearly complete 16S rRNA gene sequence, strain LMG 30035T was found to be closely related to the type strains of Actinomadura fibrosa ATCC 49459T (98.88 %) and Actinomadura formosensis JCM 7474T (98.82 %) (pairwise similarity values in parentheses).
31310199	5	28	with	%	1001:1001	arg1	strains					1013:1019	type strains	1008:1019	type strains of validly named Actinomadura species	1008:1057	Digital DNA-DNA hybridisation experiments revealed unambiguously that strain LMG 30035T represents a novel Actinomadura species (OrthoANIu values less than 83.1 %; dDDH values less than 27.2 % with type strains of validly named Actinomadura species).
31310199	5	29	theme	Actinomadura	1038:1049	arg1	species					1051:1057	validly named Actinomadura species	1024:1057	validly named Actinomadura species	1024:1057	Digital DNA-DNA hybridisation experiments revealed unambiguously that strain LMG 30035T represents a novel Actinomadura species (OrthoANIu values less than 83.1 %; dDDH values less than 27.2 % with type strains of validly named Actinomadura species).
31310199	1	30	theme	polyether	61:69	arg1	ionophore					71:79	a polyether ionophore	59:79	a polyether ionophore	59:79	nov., producer of semduramicin, a polyether ionophore.
31310199	4	31	theme	49459T	699:704	arg1	strains					662:668	the type strains	653:668	the type strains of Actinomadura fibrosa ATCC 49459T (98.88 %) and Actinomadura formosensis JCM 7474T (98.82 %) (pairwise similarity values in parentheses)	653:807	Based on analysis of nearly complete 16S rRNA gene sequence, strain LMG 30035T was found to be closely related to the type strains of Actinomadura fibrosa ATCC 49459T (98.88 %) and Actinomadura formosensis JCM 7474T (98.82 %) (pairwise similarity values in parentheses).
31310199	2	32	theme	polyphasic	326:335	arg1	approach					337:344	a polyphasic approach	324:344	a polyphasic approach	324:344	The taxonomic position of 'Actinomadura roseorufa' LMG 30035T, a semduramicin-producing mutant of strain ATCC 53666P, which was isolated from a soil sample collected in Yamae Village, Kamamoto, Japan, was clarified in the present study using a polyphasic approach.
31310199	5	33	theme	novel	911:915	arg1	species					930:936	a novel Actinomadura species	909:936	a novel Actinomadura species (OrthoANIu values less than 83.1 %; dDDH values less than 27.2 % with type strains of validly named Actinomadura species)	909:1058	Digital DNA-DNA hybridisation experiments revealed unambiguously that strain LMG 30035T represents a novel Actinomadura species (OrthoANIu values less than 83.1 %; dDDH values less than 27.2 % with type strains of validly named Actinomadura species).
31310199	13	34	with	nov.	1751:1754	arg1	30035T					1765:1770	LMG 30035T	1761:1770	LMG 30035T (=CECT 9808T,=ATCC 53664T)	1761:1797	nov. with LMG 30035T (=CECT 9808T,=ATCC 53664T) as the type strain.
31310199	2	35	theme	semduramicin-producing	147:168	arg1	mutant					170:175	a semduramicin-producing mutant	145:175	a semduramicin-producing mutant of strain ATCC 53666P, which was isolated from a soil sample collected in Yamae Village	145:263	The taxonomic position of 'Actinomadura roseorufa' LMG 30035T, a semduramicin-producing mutant of strain ATCC 53666P, which was isolated from a soil sample collected in Yamae Village, Kamamoto, Japan, was clarified in the present study using a polyphasic approach.
31310199	2	35	theme	semduramicin-producing	147:168	arg1	30035T					137:142	LMG 30035T	133:142	LMG 30035T	133:142	The taxonomic position of 'Actinomadura roseorufa' LMG 30035T, a semduramicin-producing mutant of strain ATCC 53666P, which was isolated from a soil sample collected in Yamae Village, Kamamoto, Japan, was clarified in the present study using a polyphasic approach.
31310199	12	36	theme	Actinomadura	1630:1641	arg1	species					1609:1615	validly named species	1595:1615	validly named species of the genus Actinomadura	1595:1641	In summary, these characteristics distinguish strain LMG 30035T from validly named species of the genus Actinomadura, and therefore, we propose to classify this strain formally as the novel species Actinomadura roseirufa sp.
31310199	3	37	dep	well-developed	397:410	arg1	non-fragmenting					435:449	non-fragmenting	435:449	non-fragmenting	435:449	This Gram-positive, aerobic actinomycete formed a well-developed, extensively branched, non-fragmenting substrate and aerial mycelia which differentiated into single, smooth-appearing spores.
31310199	3	37	dep	well-developed	397:410	arg1	branched					425:432	branched	425:432	branched	425:432	This Gram-positive, aerobic actinomycete formed a well-developed, extensively branched, non-fragmenting substrate and aerial mycelia which differentiated into single, smooth-appearing spores.
31310199	10	38	theme	major	1399:1403	arg1	C16 					1422:1425	C16 	1422:1425	C16 	1422:1425	The major fatty acids were C16 : 00, 10-methyl C18 : 0, C18 : 1 ω9c and C18 : 00.
31310199	10	38	theme	major	1399:1403	arg1	acids					1411:1415	The major fatty acids	1395:1415	The major fatty acids	1395:1415	The major fatty acids were C16 : 00, 10-methyl C18 : 0, C18 : 1 ω9c and C18 : 00.
31310199	2	39	theme	ATCC	187:190	arg1	mutant					170:175	a semduramicin-producing mutant	145:175	a semduramicin-producing mutant of strain ATCC 53666P, which was isolated from a soil sample collected in Yamae Village	145:263	The taxonomic position of 'Actinomadura roseorufa' LMG 30035T, a semduramicin-producing mutant of strain ATCC 53666P, which was isolated from a soil sample collected in Yamae Village, Kamamoto, Japan, was clarified in the present study using a polyphasic approach.
31310199	2	39	theme	ATCC	187:190	arg1	30035T					137:142	LMG 30035T	133:142	LMG 30035T	133:142	The taxonomic position of 'Actinomadura roseorufa' LMG 30035T, a semduramicin-producing mutant of strain ATCC 53666P, which was isolated from a soil sample collected in Yamae Village, Kamamoto, Japan, was clarified in the present study using a polyphasic approach.
31310199	4	40	theme	JCM	745:747	arg1	7474T					749:753	Actinomadura formosensis JCM 7474T	720:753	Actinomadura formosensis JCM 7474T (98.82 %) (pairwise similarity values in parentheses)	720:807	Based on analysis of nearly complete 16S rRNA gene sequence, strain LMG 30035T was found to be closely related to the type strains of Actinomadura fibrosa ATCC 49459T (98.88 %) and Actinomadura formosensis JCM 7474T (98.82 %) (pairwise similarity values in parentheses).
31310199	4	40	theme	JCM	745:747	arg1	%					762:762	98.82 %	756:762	98.82 %	756:762	Based on analysis of nearly complete 16S rRNA gene sequence, strain LMG 30035T was found to be closely related to the type strains of Actinomadura fibrosa ATCC 49459T (98.88 %) and Actinomadura formosensis JCM 7474T (98.82 %) (pairwise similarity values in parentheses).
31310199	4	41	theme	rRNA	580:583	arg1	sequence					590:597	nearly complete 16S rRNA gene sequence	560:597	nearly complete 16S rRNA gene sequence	560:597	Based on analysis of nearly complete 16S rRNA gene sequence, strain LMG 30035T was found to be closely related to the type strains of Actinomadura fibrosa ATCC 49459T (98.88 %) and Actinomadura formosensis JCM 7474T (98.82 %) (pairwise similarity values in parentheses).
31310199	13	42	theme	=ATCC	1785:1789	arg1	53664T					1791:1796	=ATCC 53664T	1785:1796	=ATCC 53664T	1785:1796	nov. with LMG 30035T (=CECT 9808T,=ATCC 53664T) as the type strain.
31310199	13	42	theme	=ATCC	1785:1789	arg1	9808T					1779:1783	=CECT 9808T	1773:1783	=CECT 9808T	1773:1783	nov. with LMG 30035T (=CECT 9808T,=ATCC 53664T) as the type strain.
31310199	4	43	theme	sequence	590:597	arg1	analysis					548:555	analysis	548:555	analysis of nearly complete 16S rRNA gene sequence	548:597	Based on analysis of nearly complete 16S rRNA gene sequence, strain LMG 30035T was found to be closely related to the type strains of Actinomadura fibrosa ATCC 49459T (98.88 %) and Actinomadura formosensis JCM 7474T (98.82 %) (pairwise similarity values in parentheses).
31310199	2	44	theme	present	304:310	arg1	study					312:316	the present study	300:316	the present study	300:316	The taxonomic position of 'Actinomadura roseorufa' LMG 30035T, a semduramicin-producing mutant of strain ATCC 53666P, which was isolated from a soil sample collected in Yamae Village, Kamamoto, Japan, was clarified in the present study using a polyphasic approach.
31310199	1	45	dep	ionophore	71:79	arg1	producer					33:40	producer	33:40	producer of semduramicin	33:56	nov., producer of semduramicin, a polyether ionophore.
31310199	1	45	dep	ionophore	71:79	arg1	nov.					27:30	nov.	27:30	nov.	27:30	nov., producer of semduramicin, a polyether ionophore.
31310199	4	46	theme	complete	567:574	arg1	rRNA					580:583	nearly complete 16S rRNA	560:583	nearly complete 16S rRNA gene sequence	560:597	Based on analysis of nearly complete 16S rRNA gene sequence, strain LMG 30035T was found to be closely related to the type strains of Actinomadura fibrosa ATCC 49459T (98.88 %) and Actinomadura formosensis JCM 7474T (98.82 %) (pairwise similarity values in parentheses).
31310199	2	47	theme	LMG	133:135	arg1	Japan					276:280	Japan	276:280	Japan	276:280	The taxonomic position of 'Actinomadura roseorufa' LMG 30035T, a semduramicin-producing mutant of strain ATCC 53666P, which was isolated from a soil sample collected in Yamae Village, Kamamoto, Japan, was clarified in the present study using a polyphasic approach.
31310199	2	47	theme	LMG	133:135	arg1	mutant					170:175	a semduramicin-producing mutant	145:175	a semduramicin-producing mutant of strain ATCC 53666P, which was isolated from a soil sample collected in Yamae Village	145:263	The taxonomic position of 'Actinomadura roseorufa' LMG 30035T, a semduramicin-producing mutant of strain ATCC 53666P, which was isolated from a soil sample collected in Yamae Village, Kamamoto, Japan, was clarified in the present study using a polyphasic approach.
31310199	2	47	theme	LMG	133:135	arg1	30035T					137:142	LMG 30035T	133:142	LMG 30035T	133:142	The taxonomic position of 'Actinomadura roseorufa' LMG 30035T, a semduramicin-producing mutant of strain ATCC 53666P, which was isolated from a soil sample collected in Yamae Village, Kamamoto, Japan, was clarified in the present study using a polyphasic approach.
31310199	3	48	theme	single	506:511	arg1	spores					531:536	single, smooth-appearing spores	506:536	single, smooth-appearing spores	506:536	This Gram-positive, aerobic actinomycete formed a well-developed, extensively branched, non-fragmenting substrate and aerial mycelia which differentiated into single, smooth-appearing spores.
31310199	4	49	theme	pairwise	766:773	arg1	values					786:791	pairwise similarity values	766:791	pairwise similarity values in parentheses	766:806	Based on analysis of nearly complete 16S rRNA gene sequence, strain LMG 30035T was found to be closely related to the type strains of Actinomadura fibrosa ATCC 49459T (98.88 %) and Actinomadura formosensis JCM 7474T (98.82 %) (pairwise similarity values in parentheses).
31310199	12	50	theme	strain	1572:1577	arg1	30035T					1583:1588	strain LMG 30035T	1572:1588	strain LMG 30035T	1572:1588	In summary, these characteristics distinguish strain LMG 30035T from validly named species of the genus Actinomadura, and therefore, we propose to classify this strain formally as the novel species Actinomadura roseirufa sp.
31310199	6	51	theme	wall	1082:1085	arg1	Analysis					1061:1068	Analysis	1061:1068	Analysis of the cell wall	1061:1085	Analysis of the cell wall revealed the presence of meso-diaminopimelic acid in the peptidoglycan.
31310199	13	52	theme	LMG	1761:1763	arg1	30035T					1765:1770	LMG 30035T	1761:1770	LMG 30035T (=CECT 9808T,=ATCC 53664T)	1761:1797	nov. with LMG 30035T (=CECT 9808T,=ATCC 53664T) as the type strain.
31310199	4	53	theme	strain	600:605	arg1	30035T					611:616	strain LMG 30035T	600:616	strain LMG 30035T	600:616	Based on analysis of nearly complete 16S rRNA gene sequence, strain LMG 30035T was found to be closely related to the type strains of Actinomadura fibrosa ATCC 49459T (98.88 %) and Actinomadura formosensis JCM 7474T (98.82 %) (pairwise similarity values in parentheses).
31310199	5	54	theme	type	1008:1011	arg1	strains					1013:1019	type strains	1008:1019	type strains of validly named Actinomadura species	1008:1057	Digital DNA-DNA hybridisation experiments revealed unambiguously that strain LMG 30035T represents a novel Actinomadura species (OrthoANIu values less than 83.1 %; dDDH values less than 27.2 % with type strains of validly named Actinomadura species).
31310199	10	55	dep	C16 	1422:1425	arg1	C18 					1451:1454	C18 	1451:1454	C18 	1451:1454	The major fatty acids were C16 : 00, 10-methyl C18 : 0, C18 : 1 ω9c and C18 : 00.
31310199	10	55	dep	C16 	1422:1425	arg1	ω9c					1459:1461	ω9c	1459:1461	ω9c	1459:1461	The major fatty acids were C16 : 00, 10-methyl C18 : 0, C18 : 1 ω9c and C18 : 00.
31310199	10	55	dep	C16 	1422:1425	arg1	 00					1472:1474	 00	1472:1474	 00	1472:1474	The major fatty acids were C16 : 00, 10-methyl C18 : 0, C18 : 1 ω9c and C18 : 00.
31310199	10	55	dep	C16 	1422:1425	arg1	C18 					1467:1470	C18 	1467:1470	C18 	1467:1470	The major fatty acids were C16 : 00, 10-methyl C18 : 0, C18 : 1 ω9c and C18 : 00.
31310199	3	56	dep	single	506:511	arg1	smooth-appearing					514:529	smooth-appearing	514:529	smooth-appearing	514:529	This Gram-positive, aerobic actinomycete formed a well-developed, extensively branched, non-fragmenting substrate and aerial mycelia which differentiated into single, smooth-appearing spores.
31310199	5	57	theme	strain	880:885	arg1	30035T					891:896	strain LMG 30035T	880:896	strain LMG 30035T	880:896	Digital DNA-DNA hybridisation experiments revealed unambiguously that strain LMG 30035T represents a novel Actinomadura species (OrthoANIu values less than 83.1 %; dDDH values less than 27.2 % with type strains of validly named Actinomadura species).
31310199	5	58	theme	Actinomadura	917:928	arg1	species					930:936	a novel Actinomadura species	909:936	a novel Actinomadura species (OrthoANIu values less than 83.1 %; dDDH values less than 27.2 % with type strains of validly named Actinomadura species)	909:1058	Digital DNA-DNA hybridisation experiments revealed unambiguously that strain LMG 30035T represents a novel Actinomadura species (OrthoANIu values less than 83.1 %; dDDH values less than 27.2 % with type strains of validly named Actinomadura species).
31310199	5	59	dep	species	930:936	arg1	values					949:954	OrthoANIu values	939:954	OrthoANIu values less than 83.1 %	939:971	Digital DNA-DNA hybridisation experiments revealed unambiguously that strain LMG 30035T represents a novel Actinomadura species (OrthoANIu values less than 83.1 %; dDDH values less than 27.2 % with type strains of validly named Actinomadura species).
31310199	7	60	theme	whole-cell	1163:1172	arg1	glucose					1186:1192	glucose	1186:1192	glucose	1186:1192	The whole-cell sugars were glucose, madurose, galactose, ribose and rhamnose.
31310199	7	60	theme	whole-cell	1163:1172	arg1	sugars					1174:1179	The whole-cell sugars	1159:1179	The whole-cell sugars	1159:1179	The whole-cell sugars were glucose, madurose, galactose, ribose and rhamnose.
31310199	5	61	theme	DNA-DNA	818:824	arg1	experiments					840:850	Digital DNA-DNA hybridisation experiments	810:850	Digital DNA-DNA hybridisation experiments	810:850	Digital DNA-DNA hybridisation experiments revealed unambiguously that strain LMG 30035T represents a novel Actinomadura species (OrthoANIu values less than 83.1 %; dDDH values less than 27.2 % with type strains of validly named Actinomadura species).
31310199	6	62	theme	acid	1132:1135	arg1	presence					1100:1107	the presence	1096:1107	the presence of meso-diaminopimelic acid in the peptidoglycan	1096:1156	Analysis of the cell wall revealed the presence of meso-diaminopimelic acid in the peptidoglycan.
31310199	8	63	theme	major	1241:1245	arg1	lipids					1253:1258	The major polar lipids	1237:1258	The major polar lipids	1237:1258	The major polar lipids included phosphatidylinositol and diphosphatidylglycerol.
31310199	12	64	theme	Actinomadura	1724:1735	arg1	strain					1687:1692	this strain	1682:1692	this strain	1682:1692	In summary, these characteristics distinguish strain LMG 30035T from validly named species of the genus Actinomadura, and therefore, we propose to classify this strain formally as the novel species Actinomadura roseirufa sp.
31310199	12	64	theme	Actinomadura	1724:1735	arg1	sp					1747:1748	the novel species Actinomadura roseirufa sp	1706:1748	the novel species Actinomadura roseirufa sp	1706:1748	In summary, these characteristics distinguish strain LMG 30035T from validly named species of the genus Actinomadura, and therefore, we propose to classify this strain formally as the novel species Actinomadura roseirufa sp.
31310199	1	65	theme	semduramicin	45:56	arg1	producer					33:40	producer	33:40	producer of semduramicin	33:56	nov., producer of semduramicin, a polyether ionophore.
31310199	1	65	theme	semduramicin	45:56	arg1	nov.					27:30	nov.	27:30	nov.	27:30	nov., producer of semduramicin, a polyether ionophore.
31310199	6	66	from	presence	1100:1107	arg1	peptidoglycan					1144:1156	the peptidoglycan	1140:1156	the peptidoglycan	1140:1156	Analysis of the cell wall revealed the presence of meso-diaminopimelic acid in the peptidoglycan.
31310199	6	67	attach	presence	1100:1107	arg2	acid					1132:1135	meso-diaminopimelic acid	1112:1135	meso-diaminopimelic acid	1112:1135	Analysis of the cell wall revealed the presence of meso-diaminopimelic acid in the peptidoglycan.
31310199	6	67	attach	presence	1100:1107	arg1	peptidoglycan					1144:1156	the peptidoglycan	1140:1156	the peptidoglycan	1140:1156	Analysis of the cell wall revealed the presence of meso-diaminopimelic acid in the peptidoglycan.
31310199	5	68	theme	named	1032:1036	arg1	species					1051:1057	validly named Actinomadura species	1024:1057	validly named Actinomadura species	1024:1057	Digital DNA-DNA hybridisation experiments revealed unambiguously that strain LMG 30035T represents a novel Actinomadura species (OrthoANIu values less than 83.1 %; dDDH values less than 27.2 % with type strains of validly named Actinomadura species).
31310199	3	69	dep	Gram-positive	352:364	arg1	aerobic					367:373	aerobic	367:373	aerobic	367:373	This Gram-positive, aerobic actinomycete formed a well-developed, extensively branched, non-fragmenting substrate and aerial mycelia which differentiated into single, smooth-appearing spores.
31310199	3	70	theme	well-developed	397:410	arg1	substrate					451:459	well-developed, extensively branched, non-fragmenting substrate	397:459	well-developed, extensively branched, non-fragmenting substrate	397:459	This Gram-positive, aerobic actinomycete formed a well-developed, extensively branched, non-fragmenting substrate and aerial mycelia which differentiated into single, smooth-appearing spores.
31310199	5	71	theme	species	1051:1057	arg1	strains					1013:1019	type strains	1008:1019	type strains of validly named Actinomadura species	1008:1057	Digital DNA-DNA hybridisation experiments revealed unambiguously that strain LMG 30035T represents a novel Actinomadura species (OrthoANIu values less than 83.1 %; dDDH values less than 27.2 % with type strains of validly named Actinomadura species).
31310199	12	72	theme	novel	1710:1714	arg1	strain					1687:1692	this strain	1682:1692	this strain	1682:1692	In summary, these characteristics distinguish strain LMG 30035T from validly named species of the genus Actinomadura, and therefore, we propose to classify this strain formally as the novel species Actinomadura roseirufa sp.
31310199	12	72	theme	novel	1710:1714	arg1	sp					1747:1748	the novel species Actinomadura roseirufa sp	1706:1748	the novel species Actinomadura roseirufa sp	1706:1748	In summary, these characteristics distinguish strain LMG 30035T from validly named species of the genus Actinomadura, and therefore, we propose to classify this strain formally as the novel species Actinomadura roseirufa sp.
31310199	4	73	from	values	786:791	arg1	parentheses					796:806	parentheses	796:806	parentheses	796:806	Based on analysis of nearly complete 16S rRNA gene sequence, strain LMG 30035T was found to be closely related to the type strains of Actinomadura fibrosa ATCC 49459T (98.88 %) and Actinomadura formosensis JCM 7474T (98.82 %) (pairwise similarity values in parentheses).
31310199	4	74	theme	ATCC	694:697	arg1	49459T					699:704	Actinomadura fibrosa ATCC 49459T	673:704	Actinomadura fibrosa ATCC 49459T (98.88 %)	673:714	Based on analysis of nearly complete 16S rRNA gene sequence, strain LMG 30035T was found to be closely related to the type strains of Actinomadura fibrosa ATCC 49459T (98.88 %) and Actinomadura formosensis JCM 7474T (98.82 %) (pairwise similarity values in parentheses).
31310199	4	74	theme	ATCC	694:697	arg1	%					713:713	98.88 %	707:713	98.88 %	707:713	Based on analysis of nearly complete 16S rRNA gene sequence, strain LMG 30035T was found to be closely related to the type strains of Actinomadura fibrosa ATCC 49459T (98.88 %) and Actinomadura formosensis JCM 7474T (98.82 %) (pairwise similarity values in parentheses).
31310199	11	75	theme	72.5 mol	1515:1522	arg1	G+C content					1485:1495	The DNA G+C content	1477:1495	The DNA G+C content of its genome	1477:1509	The DNA G+C content of its genome was 72.5 mol%.
31310199	11	75	theme	72.5 mol	1515:1522	arg1	%					1523:1523	72.5 mol%	1515:1523	72.5 mol%	1515:1523	The DNA G+C content of its genome was 72.5 mol%.
31310199	11	76	theme	genome	1504:1509	arg1	G+C content					1485:1495	The DNA G+C content	1477:1495	The DNA G+C content of its genome	1477:1509	The DNA G+C content of its genome was 72.5 mol%.
31310199	11	76	theme	genome	1504:1509	arg1	%					1523:1523	72.5 mol%	1515:1523	72.5 mol%	1515:1523	The DNA G+C content of its genome was 72.5 mol%.
31310199	9	77	theme	predominant	1322:1332	arg1	MK-9					1352:1355	MK-9	1352:1355	MK-9	1352:1355	The predominant menaquinones were MK-9(H6), MK-9(H8), MK-9(H4) and MK-9(H2).
31310199	9	77	theme	predominant	1322:1332	arg1	menaquinones					1334:1345	The predominant menaquinones	1318:1345	The predominant menaquinones	1318:1345	The predominant menaquinones were MK-9(H6), MK-9(H8), MK-9(H4) and MK-9(H2).
31310199	5	78	theme	dDDH	974:977	arg1	values					979:984	dDDH values	974:984	dDDH values less than 27.2 % with type strains of validly named Actinomadura species	974:1057	Digital DNA-DNA hybridisation experiments revealed unambiguously that strain LMG 30035T represents a novel Actinomadura species (OrthoANIu values less than 83.1 %; dDDH values less than 27.2 % with type strains of validly named Actinomadura species).
31310199	12	79	theme	genus	1624:1628	arg1	Actinomadura					1630:1641	the genus Actinomadura	1620:1641	the genus Actinomadura	1620:1641	In summary, these characteristics distinguish strain LMG 30035T from validly named species of the genus Actinomadura, and therefore, we propose to classify this strain formally as the novel species Actinomadura roseirufa sp.
31310199	3	80	theme	Gram-positive	352:364	arg1	actinomycete					375:386	This Gram-positive, aerobic actinomycete	347:386	This Gram-positive, aerobic actinomycete	347:386	This Gram-positive, aerobic actinomycete formed a well-developed, extensively branched, non-fragmenting substrate and aerial mycelia which differentiated into single, smooth-appearing spores.
31310199	2	81	dep	Actinomadura	109:120	arg1	Japan					276:280	Japan	276:280	Japan	276:280	The taxonomic position of 'Actinomadura roseorufa' LMG 30035T, a semduramicin-producing mutant of strain ATCC 53666P, which was isolated from a soil sample collected in Yamae Village, Kamamoto, Japan, was clarified in the present study using a polyphasic approach.
31310199	2	81	dep	Actinomadura	109:120	arg1	mutant					170:175	a semduramicin-producing mutant	145:175	a semduramicin-producing mutant of strain ATCC 53666P, which was isolated from a soil sample collected in Yamae Village	145:263	The taxonomic position of 'Actinomadura roseorufa' LMG 30035T, a semduramicin-producing mutant of strain ATCC 53666P, which was isolated from a soil sample collected in Yamae Village, Kamamoto, Japan, was clarified in the present study using a polyphasic approach.
31310199	2	81	dep	Actinomadura	109:120	arg1	30035T					137:142	LMG 30035T	133:142	LMG 30035T	133:142	The taxonomic position of 'Actinomadura roseorufa' LMG 30035T, a semduramicin-producing mutant of strain ATCC 53666P, which was isolated from a soil sample collected in Yamae Village, Kamamoto, Japan, was clarified in the present study using a polyphasic approach.
31310199	10	82	theme	fatty	1405:1409	arg1	C16 					1422:1425	C16 	1422:1425	C16 	1422:1425	The major fatty acids were C16 : 00, 10-methyl C18 : 0, C18 : 1 ω9c and C18 : 00.
31310199	10	82	theme	fatty	1405:1409	arg1	acids					1411:1415	The major fatty acids	1395:1415	The major fatty acids	1395:1415	The major fatty acids were C16 : 00, 10-methyl C18 : 0, C18 : 1 ω9c and C18 : 00.
31310199	13	83	theme	type	1806:1809	arg1	strain					1811:1816	the type strain	1802:1816	the type strain	1802:1816	nov. with LMG 30035T (=CECT 9808T,=ATCC 53664T) as the type strain.
31310199	3	84	theme	aerial	465:470	arg1	mycelia					472:478	aerial mycelia	465:478	aerial mycelia	465:478	This Gram-positive, aerobic actinomycete formed a well-developed, extensively branched, non-fragmenting substrate and aerial mycelia which differentiated into single, smooth-appearing spores.
31310199	4	85	theme	gene	585:588	arg1	sequence					590:597	nearly complete 16S rRNA gene sequence	560:597	nearly complete 16S rRNA gene sequence	560:597	Based on analysis of nearly complete 16S rRNA gene sequence, strain LMG 30035T was found to be closely related to the type strains of Actinomadura fibrosa ATCC 49459T (98.88 %) and Actinomadura formosensis JCM 7474T (98.82 %) (pairwise similarity values in parentheses).
31310199	5	86	theme	OrthoANIu	939:947	arg1	values					949:954	OrthoANIu values	939:954	OrthoANIu values less than 83.1 %	939:971	Digital DNA-DNA hybridisation experiments revealed unambiguously that strain LMG 30035T represents a novel Actinomadura species (OrthoANIu values less than 83.1 %; dDDH values less than 27.2 % with type strains of validly named Actinomadura species).
31310199	2	87	theme	strain	180:185	arg1	ATCC					187:190	strain ATCC 53666P	180:197	strain ATCC 53666P	180:197	The taxonomic position of 'Actinomadura roseorufa' LMG 30035T, a semduramicin-producing mutant of strain ATCC 53666P, which was isolated from a soil sample collected in Yamae Village, Kamamoto, Japan, was clarified in the present study using a polyphasic approach.
31310199	4	88	theme	formosensis	733:743	arg1	7474T					749:753	Actinomadura formosensis JCM 7474T	720:753	Actinomadura formosensis JCM 7474T (98.82 %) (pairwise similarity values in parentheses)	720:807	Based on analysis of nearly complete 16S rRNA gene sequence, strain LMG 30035T was found to be closely related to the type strains of Actinomadura fibrosa ATCC 49459T (98.88 %) and Actinomadura formosensis JCM 7474T (98.82 %) (pairwise similarity values in parentheses).
31310199	4	88	theme	formosensis	733:743	arg1	%					762:762	98.82 %	756:762	98.82 %	756:762	Based on analysis of nearly complete 16S rRNA gene sequence, strain LMG 30035T was found to be closely related to the type strains of Actinomadura fibrosa ATCC 49459T (98.88 %) and Actinomadura formosensis JCM 7474T (98.82 %) (pairwise similarity values in parentheses).
31310199	12	89	theme	named	1603:1607	arg1	species					1609:1615	validly named species	1595:1615	validly named species of the genus Actinomadura	1595:1641	In summary, these characteristics distinguish strain LMG 30035T from validly named species of the genus Actinomadura, and therefore, we propose to classify this strain formally as the novel species Actinomadura roseirufa sp.
31310199	2	90	theme	Actinomadura	109:120	arg1	position					96:103	The taxonomic position	82:103	The taxonomic position of 'Actinomadura roseorufa' LMG 30035T, a semduramicin-producing mutant of strain ATCC 53666P, which was isolated from a soil sample collected in Yamae Village, Kamamoto, Japan,	82:281	The taxonomic position of 'Actinomadura roseorufa' LMG 30035T, a semduramicin-producing mutant of strain ATCC 53666P, which was isolated from a soil sample collected in Yamae Village, Kamamoto, Japan, was clarified in the present study using a polyphasic approach.
31310199	4	91	theme	7474T	749:753	arg1	strains					662:668	the type strains	653:668	the type strains of Actinomadura fibrosa ATCC 49459T (98.88 %) and Actinomadura formosensis JCM 7474T (98.82 %) (pairwise similarity values in parentheses)	653:807	Based on analysis of nearly complete 16S rRNA gene sequence, strain LMG 30035T was found to be closely related to the type strains of Actinomadura fibrosa ATCC 49459T (98.88 %) and Actinomadura formosensis JCM 7474T (98.82 %) (pairwise similarity values in parentheses).
30801239	8	0	theme	genomic	1195:1201	arg1	species					1203:1209	a separate genomic species	1184:1209	a separate genomic species based on DNA-DNA hybridization results	1184:1248	Strain NEAU-YY265T was phenotypically distinct from recognized Jiangella species and shown to belong to a separate genomic species based on DNA-DNA hybridization results.
30801239	4	1	theme	predominant	809:819	arg1	MK-9					778:781	MK-9	778:781	MK-9	778:781	The chemotaxonomic properties of strain NEAU-YY265T were consistent with those of the genus Jiangella: the cell-wall peptidoglycan type was based on ll-diaminopimelic acid; the whole-cell hydrolysates contained glucose, ribose and rhamnose; MK-9(H4) and MK-9(H2) were the predominant menaquinones.
30801239	4	1	theme	predominant	809:819	arg1	menaquinones					821:832	the predominant menaquinones	805:832	the predominant menaquinones	805:832	The chemotaxonomic properties of strain NEAU-YY265T were consistent with those of the genus Jiangella: the cell-wall peptidoglycan type was based on ll-diaminopimelic acid; the whole-cell hydrolysates contained glucose, ribose and rhamnose; MK-9(H4) and MK-9(H2) were the predominant menaquinones.
30801239	4	1	theme	predominant	809:819	arg1	MK-9					791:794	MK-9	791:794	MK-9	791:794	The chemotaxonomic properties of strain NEAU-YY265T were consistent with those of the genus Jiangella: the cell-wall peptidoglycan type was based on ll-diaminopimelic acid; the whole-cell hydrolysates contained glucose, ribose and rhamnose; MK-9(H4) and MK-9(H2) were the predominant menaquinones.
30801239	3	2	theme	Phylogenetic	285:296	arg1	analyses					298:305	Phylogenetic analyses	285:305	Phylogenetic analyses based on 16S rRNA gene sequences	285:338	Phylogenetic analyses based on 16S rRNA gene sequences showed that strain NEAU-YY265T belonged to the genus Jiangella and exhibited 16S rRNA gene sequence similarities of 99.1 and 99.1 % to Jiangella mangrovi 3SM4-07T and Jiangella alkaliphila D8-87T.
30801239	9	3	theme	rhizosphaerae	1372:1384	arg1	sp					1386:1387	the name Jiangella rhizosphaerae sp	1353:1387	the name Jiangella rhizosphaerae sp	1353:1387	Thus, strain NEAU-YY265T is considered to represent a novel species of the genus Jiangella, for which the name Jiangella rhizosphaerae sp.
30801239	3	4	theme	%	470:470	arg1	similarities					440:451	16S rRNA gene sequence similarities	417:451	16S rRNA gene sequence similarities of 99.1 and 99.1 %	417:470	Phylogenetic analyses based on 16S rRNA gene sequences showed that strain NEAU-YY265T belonged to the genus Jiangella and exhibited 16S rRNA gene sequence similarities of 99.1 and 99.1 % to Jiangella mangrovi 3SM4-07T and Jiangella alkaliphila D8-87T.
30801239	4	5	theme	chemotaxonomic	541:554	arg1	consistent					594:603	consistent	594:603	consistent	594:603	The chemotaxonomic properties of strain NEAU-YY265T were consistent with those of the genus Jiangella: the cell-wall peptidoglycan type was based on ll-diaminopimelic acid; the whole-cell hydrolysates contained glucose, ribose and rhamnose; MK-9(H4) and MK-9(H2) were the predominant menaquinones.
30801239	4	5	theme	chemotaxonomic	541:554	arg1	properties					556:565	The chemotaxonomic properties	537:565	The chemotaxonomic properties of strain NEAU-YY265T	537:587	The chemotaxonomic properties of strain NEAU-YY265T were consistent with those of the genus Jiangella: the cell-wall peptidoglycan type was based on ll-diaminopimelic acid; the whole-cell hydrolysates contained glucose, ribose and rhamnose; MK-9(H4) and MK-9(H2) were the predominant menaquinones.
30801239	3	6	theme	rRNA	320:323	arg1	sequences					330:338	16S rRNA gene sequences	316:338	16S rRNA gene sequences	316:338	Phylogenetic analyses based on 16S rRNA gene sequences showed that strain NEAU-YY265T belonged to the genus Jiangella and exhibited 16S rRNA gene sequence similarities of 99.1 and 99.1 % to Jiangella mangrovi 3SM4-07T and Jiangella alkaliphila D8-87T.
30801239	6	7	dep	 0	991:992	arg1	 0					1041:1042	 0	1041:1042	 0	1041:1042	The major fatty acids (>5 %) were anteiso-C15 : 0, anteiso-C17 : 0, C17 : 1ω8c, iso-C16 : 0, C17 : 0 and iso-C15 : 0.
30801239	6	7	dep	 0	991:992	arg1	iso-C15 					1032:1039	iso-C15 	1032:1039	iso-C15 	1032:1039	The major fatty acids (>5 %) were anteiso-C15 : 0, anteiso-C17 : 0, C17 : 1ω8c, iso-C16 : 0, C17 : 0 and iso-C15 : 0.
30801239	6	7	dep	 0	991:992	arg1	 0					1025:1026	 0	1025:1026	 0	1025:1026	The major fatty acids (>5 %) were anteiso-C15 : 0, anteiso-C17 : 0, C17 : 1ω8c, iso-C16 : 0, C17 : 0 and iso-C15 : 0.
30801239	6	7	dep	 0	991:992	arg1	C17 					1020:1023	C17 	1020:1023	C17 	1020:1023	The major fatty acids (>5 %) were anteiso-C15 : 0, anteiso-C17 : 0, C17 : 1ω8c, iso-C16 : 0, C17 : 0 and iso-C15 : 0.
30801239	11	8	theme	type	1412:1415	arg1	NEAU-YY265T					1427:1437	NEAU-YY265T	1427:1437	NEAU-YY265T (=JCM 32551T=CGMCC 4.7475T)	1427:1465	The type strain is NEAU-YY265T (=JCM 32551T=CGMCC 4.7475T).
30801239	11	8	theme	type	1412:1415	arg1	strain					1417:1422	The type strain	1408:1422	The type strain	1408:1422	The type strain is NEAU-YY265T (=JCM 32551T=CGMCC 4.7475T).
30801239	4	9	theme	strain	570:575	arg1	NEAU-YY265T					577:587	strain NEAU-YY265T	570:587	strain NEAU-YY265T	570:587	The chemotaxonomic properties of strain NEAU-YY265T were consistent with those of the genus Jiangella: the cell-wall peptidoglycan type was based on ll-diaminopimelic acid; the whole-cell hydrolysates contained glucose, ribose and rhamnose; MK-9(H4) and MK-9(H2) were the predominant menaquinones.
30801239	3	10	theme	gene	325:328	arg1	sequences					330:338	16S rRNA gene sequences	316:338	16S rRNA gene sequences	316:338	Phylogenetic analyses based on 16S rRNA gene sequences showed that strain NEAU-YY265T belonged to the genus Jiangella and exhibited 16S rRNA gene sequence similarities of 99.1 and 99.1 % to Jiangella mangrovi 3SM4-07T and Jiangella alkaliphila D8-87T.
30801239	2	11	theme	novel	120:124	arg1	actinomycete					126:137	A novel actinomycete	118:137	A novel actinomycete	118:137	A novel actinomycete, designated strain NEAU-YY265T, was isolated from rhizosphere soil of wheat (Triticum aestivum L.) and characterized using a polyphasic approach.
30801239	5	12	dep	fragmented	886:895	arg1	mycelium					871:878	branching mycelium	861:878	branching mycelium which fragmented into short or elongated rods	861:924	The novel strain produced branching mycelium which fragmented into short or elongated rods.
30801239	0	13	theme	Jiangella	0:8	arg1	sp					24:25	Jiangella rhizosphaerae sp	0:25	Jiangella rhizosphaerae sp.	0:26	Jiangella rhizosphaerae sp.
30801239	9	14	theme	Jiangella	1332:1340	arg1	species					1311:1317	a novel species	1303:1317	a novel species	1303:1317	Thus, strain NEAU-YY265T is considered to represent a novel species of the genus Jiangella, for which the name Jiangella rhizosphaerae sp.
30801239	6	15	theme	fatty	937:941	arg1	anteiso-C17 					978:989	anteiso-C17 	978:989	anteiso-C17 	978:989	The major fatty acids (>5 %) were anteiso-C15 : 0, anteiso-C17 : 0, C17 : 1ω8c, iso-C16 : 0, C17 : 0 and iso-C15 : 0.
30801239	6	15	theme	fatty	937:941	arg1	acids					943:947	The major fatty acids	927:947	The major fatty acids (>5 %)	927:954	The major fatty acids (>5 %) were anteiso-C15 : 0, anteiso-C17 : 0, C17 : 1ω8c, iso-C16 : 0, C17 : 0 and iso-C15 : 0.
30801239	6	15	theme	fatty	937:941	arg1	%					953:953	>5 %	950:953	>5 %	950:953	The major fatty acids (>5 %) were anteiso-C15 : 0, anteiso-C17 : 0, C17 : 1ω8c, iso-C16 : 0, C17 : 0 and iso-C15 : 0.
30801239	7	16	theme	DNA	1049:1051	arg1	G+C content					1053:1063	The DNA G+C content	1045:1063	The DNA G+C content	1045:1063	The DNA G+C content was 72.6 mol%.
30801239	7	16	theme	DNA	1049:1051	arg1	%					1077:1077	72.6 mol%	1069:1077	72.6 mol%	1069:1077	The DNA G+C content was 72.6 mol%.
30801239	5	17	theme	branching	861:869	arg1	mycelium					871:878	branching mycelium	861:878	branching mycelium which fragmented into short or elongated rods	861:924	The novel strain produced branching mycelium which fragmented into short or elongated rods.
30801239	3	18	theme	sequence	431:438	arg1	similarities					440:451	16S rRNA gene sequence similarities	417:451	16S rRNA gene sequence similarities of 99.1 and 99.1 %	417:470	Phylogenetic analyses based on 16S rRNA gene sequences showed that strain NEAU-YY265T belonged to the genus Jiangella and exhibited 16S rRNA gene sequence similarities of 99.1 and 99.1 % to Jiangella mangrovi 3SM4-07T and Jiangella alkaliphila D8-87T.
30801239	1	19	dep	Triticum	95:102	arg1	L					113:113	L	113:113	Triticum aestivum L.	95:114	nov., an actinomycete isolated from the rhizosphere soil of wheat (Triticum aestivum L.).
30801239	1	19	dep	Triticum	95:102	arg1	aestivum					104:111	Triticum aestivum L.	95:114	Triticum aestivum L.	95:114	nov., an actinomycete isolated from the rhizosphere soil of wheat (Triticum aestivum L.).
30801239	4	20	theme	cell-wall	644:652	arg1	type					668:671	the cell-wall peptidoglycan type	640:671	the cell-wall peptidoglycan type	640:671	The chemotaxonomic properties of strain NEAU-YY265T were consistent with those of the genus Jiangella: the cell-wall peptidoglycan type was based on ll-diaminopimelic acid; the whole-cell hydrolysates contained glucose, ribose and rhamnose; MK-9(H4) and MK-9(H2) were the predominant menaquinones.
30801239	3	21	theme	16S	316:318	arg1	sequences					330:338	16S rRNA gene sequences	316:338	16S rRNA gene sequences	316:338	Phylogenetic analyses based on 16S rRNA gene sequences showed that strain NEAU-YY265T belonged to the genus Jiangella and exhibited 16S rRNA gene sequence similarities of 99.1 and 99.1 % to Jiangella mangrovi 3SM4-07T and Jiangella alkaliphila D8-87T.
30801239	1	22	theme	rhizosphere	68:78	arg1	soil					80:83	the rhizosphere soil	64:83	the rhizosphere soil of wheat (Triticum aestivum L.)	64:115	nov., an actinomycete isolated from the rhizosphere soil of wheat (Triticum aestivum L.).
30801239	2	23	attach	isolated	175:182	arg1	soil					201:204	rhizosphere soil	189:204	rhizosphere soil of wheat (Triticum aestivum L.)	189:236	A novel actinomycete, designated strain NEAU-YY265T, was isolated from rhizosphere soil of wheat (Triticum aestivum L.) and characterized using a polyphasic approach.
30801239	2	23	attach	isolated	175:182	arg2	actinomycete					126:137	A novel actinomycete	118:137	A novel actinomycete	118:137	A novel actinomycete, designated strain NEAU-YY265T, was isolated from rhizosphere soil of wheat (Triticum aestivum L.) and characterized using a polyphasic approach.
30801239	0	24	theme	rhizosphaerae	10:22	arg1	sp					24:25	Jiangella rhizosphaerae sp	0:25	Jiangella rhizosphaerae sp.	0:26	Jiangella rhizosphaerae sp.
30801239	4	25	theme	whole-cell	714:723	arg1	hydrolysates					725:736	the whole-cell hydrolysates	710:736	the whole-cell hydrolysates	710:736	The chemotaxonomic properties of strain NEAU-YY265T were consistent with those of the genus Jiangella: the cell-wall peptidoglycan type was based on ll-diaminopimelic acid; the whole-cell hydrolysates contained glucose, ribose and rhamnose; MK-9(H4) and MK-9(H2) were the predominant menaquinones.
30801239	3	26	theme	genus	387:391	arg1	Jiangella					393:401	the genus Jiangella	383:401	the genus Jiangella	383:401	Phylogenetic analyses based on 16S rRNA gene sequences showed that strain NEAU-YY265T belonged to the genus Jiangella and exhibited 16S rRNA gene sequence similarities of 99.1 and 99.1 % to Jiangella mangrovi 3SM4-07T and Jiangella alkaliphila D8-87T.
30801239	3	27	theme	strain	352:357	arg1	NEAU-YY265T					359:369	strain NEAU-YY265T	352:369	strain NEAU-YY265T	352:369	Phylogenetic analyses based on 16S rRNA gene sequences showed that strain NEAU-YY265T belonged to the genus Jiangella and exhibited 16S rRNA gene sequence similarities of 99.1 and 99.1 % to Jiangella mangrovi 3SM4-07T and Jiangella alkaliphila D8-87T.
30801239	6	28	theme	major	931:935	arg1	anteiso-C17 					978:989	anteiso-C17 	978:989	anteiso-C17 	978:989	The major fatty acids (>5 %) were anteiso-C15 : 0, anteiso-C17 : 0, C17 : 1ω8c, iso-C16 : 0, C17 : 0 and iso-C15 : 0.
30801239	6	28	theme	major	931:935	arg1	acids					943:947	The major fatty acids	927:947	The major fatty acids (>5 %)	927:954	The major fatty acids (>5 %) were anteiso-C15 : 0, anteiso-C17 : 0, C17 : 1ω8c, iso-C16 : 0, C17 : 0 and iso-C15 : 0.
30801239	6	28	theme	major	931:935	arg1	%					953:953	>5 %	950:953	>5 %	950:953	The major fatty acids (>5 %) were anteiso-C15 : 0, anteiso-C17 : 0, C17 : 1ω8c, iso-C16 : 0, C17 : 0 and iso-C15 : 0.
30801239	2	29	theme	polyphasic	264:273	arg1	approach					275:282	a polyphasic approach	262:282	a polyphasic approach	262:282	A novel actinomycete, designated strain NEAU-YY265T, was isolated from rhizosphere soil of wheat (Triticum aestivum L.) and characterized using a polyphasic approach.
30801239	8	30	theme	hybridization	1228:1240	arg1	results					1242:1248	DNA-DNA hybridization results	1220:1248	DNA-DNA hybridization results	1220:1248	Strain NEAU-YY265T was phenotypically distinct from recognized Jiangella species and shown to belong to a separate genomic species based on DNA-DNA hybridization results.
30801239	2	31	dep	Triticum	216:223	arg1	L.					234:235	Triticum aestivum L.	216:235	Triticum aestivum L.	216:235	A novel actinomycete, designated strain NEAU-YY265T, was isolated from rhizosphere soil of wheat (Triticum aestivum L.) and characterized using a polyphasic approach.
30801239	1	32	attach	isolated	50:57	arg2	actinomycete					37:48	an actinomycete	34:48	an actinomycete isolated from the rhizosphere soil of wheat (Triticum aestivum L.)	34:115	nov., an actinomycete isolated from the rhizosphere soil of wheat (Triticum aestivum L.).
30801239	1	32	attach	isolated	50:57	arg1	soil					80:83	the rhizosphere soil	64:83	the rhizosphere soil of wheat (Triticum aestivum L.)	64:115	nov., an actinomycete isolated from the rhizosphere soil of wheat (Triticum aestivum L.).
30801239	1	33	theme	wheat	88:92	arg1	soil					80:83	the rhizosphere soil	64:83	the rhizosphere soil of wheat (Triticum aestivum L.)	64:115	nov., an actinomycete isolated from the rhizosphere soil of wheat (Triticum aestivum L.).
30801239	3	34	theme	gene	426:429	arg1	similarities					440:451	16S rRNA gene sequence similarities	417:451	16S rRNA gene sequence similarities of 99.1 and 99.1 %	417:470	Phylogenetic analyses based on 16S rRNA gene sequences showed that strain NEAU-YY265T belonged to the genus Jiangella and exhibited 16S rRNA gene sequence similarities of 99.1 and 99.1 % to Jiangella mangrovi 3SM4-07T and Jiangella alkaliphila D8-87T.
30801239	5	35	theme	short	902:906	arg1	rods					921:924	short or elongated rods	902:924	short or elongated rods	902:924	The novel strain produced branching mycelium which fragmented into short or elongated rods.
30801239	6	36	dep	anteiso-C17 	978:989	arg1	 0					991:992	 0	991:992	 0	991:992	The major fatty acids (>5 %) were anteiso-C15 : 0, anteiso-C17 : 0, C17 : 1ω8c, iso-C16 : 0, C17 : 0 and iso-C15 : 0.
30801239	6	36	dep	anteiso-C17 	978:989	arg1	C17 					995:998	C17 	995:998	C17 	995:998	The major fatty acids (>5 %) were anteiso-C15 : 0, anteiso-C17 : 0, C17 : 1ω8c, iso-C16 : 0, C17 : 0 and iso-C15 : 0.
30801239	2	37	theme	strain	151:156	arg1	NEAU-YY265T					158:168	strain NEAU-YY265T	151:168	strain NEAU-YY265T	151:168	A novel actinomycete, designated strain NEAU-YY265T, was isolated from rhizosphere soil of wheat (Triticum aestivum L.) and characterized using a polyphasic approach.
30801239	5	38	theme	elongated	911:919	arg1	rods					921:924	short or elongated rods	902:924	short or elongated rods	902:924	The novel strain produced branching mycelium which fragmented into short or elongated rods.
30801239	8	39	from	species	1153:1159	arg1	distinct					1118:1125	distinct	1118:1125	distinct	1118:1125	Strain NEAU-YY265T was phenotypically distinct from recognized Jiangella species and shown to belong to a separate genomic species based on DNA-DNA hybridization results.
30801239	1	40	dep	actinomycete	37:48	arg1	nov.					28:31	nov.	28:31	nov.	28:31	nov., an actinomycete isolated from the rhizosphere soil of wheat (Triticum aestivum L.).
30801239	4	41	theme	genus	623:627	arg1	Jiangella					629:637	the genus Jiangella	619:637	the genus Jiangella	619:637	The chemotaxonomic properties of strain NEAU-YY265T were consistent with those of the genus Jiangella: the cell-wall peptidoglycan type was based on ll-diaminopimelic acid; the whole-cell hydrolysates contained glucose, ribose and rhamnose; MK-9(H4) and MK-9(H2) were the predominant menaquinones.
30801239	8	42	theme	recognized	1132:1141	arg1	species					1153:1159	recognized Jiangella species	1132:1159	recognized Jiangella species	1132:1159	Strain NEAU-YY265T was phenotypically distinct from recognized Jiangella species and shown to belong to a separate genomic species based on DNA-DNA hybridization results.
30801239	9	43	theme	genus	1326:1330	arg1	Jiangella					1332:1340	the genus Jiangella	1322:1340	the genus Jiangella	1322:1340	Thus, strain NEAU-YY265T is considered to represent a novel species of the genus Jiangella, for which the name Jiangella rhizosphaerae sp.
30801239	3	44	theme	16S	417:419	arg1	similarities					440:451	16S rRNA gene sequence similarities	417:451	16S rRNA gene sequence similarities of 99.1 and 99.1 %	417:470	Phylogenetic analyses based on 16S rRNA gene sequences showed that strain NEAU-YY265T belonged to the genus Jiangella and exhibited 16S rRNA gene sequence similarities of 99.1 and 99.1 % to Jiangella mangrovi 3SM4-07T and Jiangella alkaliphila D8-87T.
30801239	4	45	with	consistent	594:603	arg1	those					610:614	those	610:614	those	610:614	The chemotaxonomic properties of strain NEAU-YY265T were consistent with those of the genus Jiangella: the cell-wall peptidoglycan type was based on ll-diaminopimelic acid; the whole-cell hydrolysates contained glucose, ribose and rhamnose; MK-9(H4) and MK-9(H2) were the predominant menaquinones.
30801239	3	46	theme	rRNA	421:424	arg1	similarities					440:451	16S rRNA gene sequence similarities	417:451	16S rRNA gene sequence similarities of 99.1 and 99.1 %	417:470	Phylogenetic analyses based on 16S rRNA gene sequences showed that strain NEAU-YY265T belonged to the genus Jiangella and exhibited 16S rRNA gene sequence similarities of 99.1 and 99.1 % to Jiangella mangrovi 3SM4-07T and Jiangella alkaliphila D8-87T.
30801239	4	47	contain	contained	738:746	arg1	hydrolysates					725:736	the whole-cell hydrolysates	710:736	the whole-cell hydrolysates	710:736	The chemotaxonomic properties of strain NEAU-YY265T were consistent with those of the genus Jiangella: the cell-wall peptidoglycan type was based on ll-diaminopimelic acid; the whole-cell hydrolysates contained glucose, ribose and rhamnose; MK-9(H4) and MK-9(H2) were the predominant menaquinones.
30801239	4	47	contain	contained	738:746	arg2	rhamnose					768:775	rhamnose	768:775	rhamnose	768:775	The chemotaxonomic properties of strain NEAU-YY265T were consistent with those of the genus Jiangella: the cell-wall peptidoglycan type was based on ll-diaminopimelic acid; the whole-cell hydrolysates contained glucose, ribose and rhamnose; MK-9(H4) and MK-9(H2) were the predominant menaquinones.
30801239	4	47	contain	contained	738:746	arg2	ribose					757:762	ribose	757:762	ribose	757:762	The chemotaxonomic properties of strain NEAU-YY265T were consistent with those of the genus Jiangella: the cell-wall peptidoglycan type was based on ll-diaminopimelic acid; the whole-cell hydrolysates contained glucose, ribose and rhamnose; MK-9(H4) and MK-9(H2) were the predominant menaquinones.
30801239	4	47	contain	contained	738:746	arg2	glucose					748:754	glucose	748:754	glucose	748:754	The chemotaxonomic properties of strain NEAU-YY265T were consistent with those of the genus Jiangella: the cell-wall peptidoglycan type was based on ll-diaminopimelic acid; the whole-cell hydrolysates contained glucose, ribose and rhamnose; MK-9(H4) and MK-9(H2) were the predominant menaquinones.
30801239	2	48	theme	rhizosphere	189:199	arg1	soil					201:204	rhizosphere soil	189:204	rhizosphere soil of wheat (Triticum aestivum L.)	189:236	A novel actinomycete, designated strain NEAU-YY265T, was isolated from rhizosphere soil of wheat (Triticum aestivum L.) and characterized using a polyphasic approach.
30801239	4	49	theme	ll-diaminopimelic	686:702	arg1	acid					704:707	ll-diaminopimelic acid	686:707	ll-diaminopimelic acid	686:707	The chemotaxonomic properties of strain NEAU-YY265T were consistent with those of the genus Jiangella: the cell-wall peptidoglycan type was based on ll-diaminopimelic acid; the whole-cell hydrolysates contained glucose, ribose and rhamnose; MK-9(H4) and MK-9(H2) were the predominant menaquinones.
30801239	8	50	theme	Strain	1080:1085	arg1	NEAU-YY265T					1087:1097	Strain NEAU-YY265T	1080:1097	Strain NEAU-YY265T	1080:1097	Strain NEAU-YY265T was phenotypically distinct from recognized Jiangella species and shown to belong to a separate genomic species based on DNA-DNA hybridization results.
30801239	11	51	theme	=JCM	1440:1443	arg1	4.7475T					1458:1464	=JCM 32551T=CGMCC 4.7475T	1440:1464	=JCM 32551T=CGMCC 4.7475T	1440:1464	The type strain is NEAU-YY265T (=JCM 32551T=CGMCC 4.7475T).
30801239	11	51	theme	=JCM	1440:1443	arg1	NEAU-YY265T					1427:1437	NEAU-YY265T	1427:1437	NEAU-YY265T (=JCM 32551T=CGMCC 4.7475T)	1427:1465	The type strain is NEAU-YY265T (=JCM 32551T=CGMCC 4.7475T).
30801239	9	52	theme	name	1357:1360	arg1	sp					1386:1387	the name Jiangella rhizosphaerae sp	1353:1387	the name Jiangella rhizosphaerae sp	1353:1387	Thus, strain NEAU-YY265T is considered to represent a novel species of the genus Jiangella, for which the name Jiangella rhizosphaerae sp.
30801239	9	53	theme	novel	1305:1309	arg1	species					1311:1317	a novel species	1303:1317	a novel species	1303:1317	Thus, strain NEAU-YY265T is considered to represent a novel species of the genus Jiangella, for which the name Jiangella rhizosphaerae sp.
30801239	11	54	theme	32551T=CGMCC	1445:1456	arg1	4.7475T					1458:1464	=JCM 32551T=CGMCC 4.7475T	1440:1464	=JCM 32551T=CGMCC 4.7475T	1440:1464	The type strain is NEAU-YY265T (=JCM 32551T=CGMCC 4.7475T).
30801239	11	54	theme	32551T=CGMCC	1445:1456	arg1	NEAU-YY265T					1427:1437	NEAU-YY265T	1427:1437	NEAU-YY265T (=JCM 32551T=CGMCC 4.7475T)	1427:1465	The type strain is NEAU-YY265T (=JCM 32551T=CGMCC 4.7475T).
30801239	9	55	theme	Jiangella	1362:1370	arg1	sp					1386:1387	the name Jiangella rhizosphaerae sp	1353:1387	the name Jiangella rhizosphaerae sp	1353:1387	Thus, strain NEAU-YY265T is considered to represent a novel species of the genus Jiangella, for which the name Jiangella rhizosphaerae sp.
30801239	9	56	theme	strain	1257:1262	arg1	NEAU-YY265T					1264:1274	strain NEAU-YY265T	1257:1274	strain NEAU-YY265T	1257:1274	Thus, strain NEAU-YY265T is considered to represent a novel species of the genus Jiangella, for which the name Jiangella rhizosphaerae sp.
30801239	8	57	theme	DNA-DNA	1220:1226	arg1	hybridization					1228:1240	DNA-DNA hybridization	1220:1240	DNA-DNA hybridization results	1220:1248	Strain NEAU-YY265T was phenotypically distinct from recognized Jiangella species and shown to belong to a separate genomic species based on DNA-DNA hybridization results.
30801239	5	58	theme	novel	839:843	arg1	strain					845:850	The novel strain	835:850	The novel strain	835:850	The novel strain produced branching mycelium which fragmented into short or elongated rods.
30801239	4	59	theme	NEAU-YY265T	577:587	arg1	consistent					594:603	consistent	594:603	consistent	594:603	The chemotaxonomic properties of strain NEAU-YY265T were consistent with those of the genus Jiangella: the cell-wall peptidoglycan type was based on ll-diaminopimelic acid; the whole-cell hydrolysates contained glucose, ribose and rhamnose; MK-9(H4) and MK-9(H2) were the predominant menaquinones.
30801239	4	59	theme	NEAU-YY265T	577:587	arg1	properties					556:565	The chemotaxonomic properties	537:565	The chemotaxonomic properties of strain NEAU-YY265T	537:587	The chemotaxonomic properties of strain NEAU-YY265T were consistent with those of the genus Jiangella: the cell-wall peptidoglycan type was based on ll-diaminopimelic acid; the whole-cell hydrolysates contained glucose, ribose and rhamnose; MK-9(H4) and MK-9(H2) were the predominant menaquinones.
30801239	8	60	theme	Jiangella	1143:1151	arg1	species					1153:1159	recognized Jiangella species	1132:1159	recognized Jiangella species	1132:1159	Strain NEAU-YY265T was phenotypically distinct from recognized Jiangella species and shown to belong to a separate genomic species based on DNA-DNA hybridization results.
30801239	8	61	theme	separate	1186:1193	arg1	species					1203:1209	a separate genomic species	1184:1209	a separate genomic species based on DNA-DNA hybridization results	1184:1248	Strain NEAU-YY265T was phenotypically distinct from recognized Jiangella species and shown to belong to a separate genomic species based on DNA-DNA hybridization results.
30801239	2	62	theme	wheat	209:213	arg1	soil					201:204	rhizosphere soil	189:204	rhizosphere soil of wheat (Triticum aestivum L.)	189:236	A novel actinomycete, designated strain NEAU-YY265T, was isolated from rhizosphere soil of wheat (Triticum aestivum L.) and characterized using a polyphasic approach.
30801239	4	63	theme	peptidoglycan	654:666	arg1	type					668:671	the cell-wall peptidoglycan type	640:671	the cell-wall peptidoglycan type	640:671	The chemotaxonomic properties of strain NEAU-YY265T were consistent with those of the genus Jiangella: the cell-wall peptidoglycan type was based on ll-diaminopimelic acid; the whole-cell hydrolysates contained glucose, ribose and rhamnose; MK-9(H4) and MK-9(H2) were the predominant menaquinones.
30801239	7	64	theme	72.6 mol	1069:1076	arg1	G+C content					1053:1063	The DNA G+C content	1045:1063	The DNA G+C content	1045:1063	The DNA G+C content was 72.6 mol%.
30801239	7	64	theme	72.6 mol	1069:1076	arg1	%					1077:1077	72.6 mol%	1069:1077	72.6 mol%	1069:1077	The DNA G+C content was 72.6 mol%.
30117800	8	0	theme	phenylalanyl-tRNA	896:912	arg1	synthase					914:921	phenylalanyl-tRNA synthase	896:921	phenylalanyl-tRNA synthase alpha subunit (pheS)	896:942	A comparison of two housekeeping genes, RNA polymerase alpha subunit (rpoA) and phenylalanyl-tRNA synthase alpha subunit (pheS), revealed that strain SG816T formed a separate branch within the clade of the genus Lactobacillus.
30117800	5	1	theme	spectometry	496:506	arg1	profiling					508:516	Comparative 16S rRNA gene sequencing and the matrix-associated laser desorption/ionization-time-of-flight mass spectometry profiling	385:516	Comparative 16S rRNA gene sequencing and the matrix-associated laser desorption/ionization-time-of-flight mass spectometry profiling	385:516	Comparative 16S rRNA gene sequencing and the matrix-associated laser desorption/ionization-time-of-flight mass spectometry profiling showed that strain SG816T was closely related to Lactobacillus delbrueckiisubsp.
30117800	9	2	theme	DNA	1047:1049	arg1	%					1102:1102	51.5 mol%	1094:1102	51.5 mol%	1094:1102	The DNA G+C content level of the strain SG816T was 51.5 mol%.
30117800	9	2	theme	DNA	1047:1049	arg1	level					1063:1067	The DNA G+C content level	1043:1067	The DNA G+C content level of the strain SG816T	1043:1088	The DNA G+C content level of the strain SG816T was 51.5 mol%.
30117800	9	3	theme	51.5 mol	1094:1101	arg1	%					1102:1102	51.5 mol%	1094:1102	51.5 mol%	1094:1102	The DNA G+C content level of the strain SG816T was 51.5 mol%.
30117800	9	3	theme	51.5 mol	1094:1101	arg1	level					1063:1067	The DNA G+C content level	1043:1067	The DNA G+C content level of the strain SG816T	1043:1088	The DNA G+C content level of the strain SG816T was 51.5 mol%.
30117800	8	4	theme	strain	959:964	arg1	SG816T					966:971	strain SG816T	959:971	strain SG816T	959:971	A comparison of two housekeeping genes, RNA polymerase alpha subunit (rpoA) and phenylalanyl-tRNA synthase alpha subunit (pheS), revealed that strain SG816T formed a separate branch within the clade of the genus Lactobacillus.
30117800	6	5	theme	KCTC	610:613	arg1	3635T					615:619	bulgaricus KCTC 3635T	599:619	bulgaricus KCTC 3635T (95.9 %)	599:628	bulgaricus KCTC 3635T (95.9 %) and Lactobacillus delbrueckiisubsp.
30117800	6	5	theme	KCTC	610:613	arg1	%					627:627	95.9 %	622:627	95.9 %	622:627	bulgaricus KCTC 3635T (95.9 %) and Lactobacillus delbrueckiisubsp.
30117800	2	6	theme	anaerobic	88:96	arg1	bacterium					162:170	A facultatively anaerobic, Gram-stain-positive, catalase-negative, rod-shaped lactic acid bacterium	72:170	bacterium	162:170	A facultatively anaerobic, Gram-stain-positive, catalase-negative, rod-shaped lactic acid bacterium, designated SG816T, was isolated from small intestine of a swine.
30117800	11	7	theme	cellular	1201:1208	arg1	acids					1216:1220	The major cellular fatty acids	1191:1220	The major cellular fatty acids (>10 %) of the isolate	1191:1243	The major cellular fatty acids (>10 %) of the isolate were C18 : 1ω9c and C16 : 0.
30117800	11	7	theme	cellular	1201:1208	arg1	%					1227:1227	>10 %	1223:1227	>10 %	1223:1227	The major cellular fatty acids (>10 %) of the isolate were C18 : 1ω9c and C16 : 0.
30117800	11	7	theme	cellular	1201:1208	arg1	C18 					1250:1253	C18 	1250:1253	C18 	1250:1253	The major cellular fatty acids (>10 %) of the isolate were C18 : 1ω9c and C16 : 0.
30117800	5	8	theme	Lactobacillus	567:579	arg1	delbrueckiisubsp					581:596	Lactobacillus delbrueckiisubsp	567:596	Lactobacillus delbrueckiisubsp	567:596	Comparative 16S rRNA gene sequencing and the matrix-associated laser desorption/ionization-time-of-flight mass spectometry profiling showed that strain SG816T was closely related to Lactobacillus delbrueckiisubsp.
30117800	1	9	theme	small	44:48	arg1	intestine					50:58	small intestine	44:58	small intestine of a swine	44:69	nov., isolated from small intestine of a swine.
30117800	6	10	theme	bulgaricus	599:608	arg1	3635T					615:619	bulgaricus KCTC 3635T	599:619	bulgaricus KCTC 3635T (95.9 %)	599:628	bulgaricus KCTC 3635T (95.9 %) and Lactobacillus delbrueckiisubsp.
30117800	6	10	theme	bulgaricus	599:608	arg1	%					627:627	95.9 %	622:627	95.9 %	622:627	bulgaricus KCTC 3635T (95.9 %) and Lactobacillus delbrueckiisubsp.
30117800	8	11	theme	polymerase	860:869	arg1	rpoA					886:889	rpoA	886:889	rpoA	886:889	A comparison of two housekeeping genes, RNA polymerase alpha subunit (rpoA) and phenylalanyl-tRNA synthase alpha subunit (pheS), revealed that strain SG816T formed a separate branch within the clade of the genus Lactobacillus.
30117800	8	11	theme	polymerase	860:869	arg1	subunit					877:883	RNA polymerase alpha subunit	856:883	RNA polymerase alpha subunit (rpoA)	856:890	A comparison of two housekeeping genes, RNA polymerase alpha subunit (rpoA) and phenylalanyl-tRNA synthase alpha subunit (pheS), revealed that strain SG816T formed a separate branch within the clade of the genus Lactobacillus.
30117800	8	11	theme	polymerase	860:869	arg1	genes					849:853	two housekeeping genes	832:853	two housekeeping genes	832:853	A comparison of two housekeeping genes, RNA polymerase alpha subunit (rpoA) and phenylalanyl-tRNA synthase alpha subunit (pheS), revealed that strain SG816T formed a separate branch within the clade of the genus Lactobacillus.
30117800	9	12	theme	strain	1076:1081	arg1	SG816T					1083:1088	the strain SG816T	1072:1088	the strain SG816T	1072:1088	The DNA G+C content level of the strain SG816T was 51.5 mol%.
30117800	2	13	theme	lactic	150:155	arg1	bacterium					162:170	A facultatively anaerobic, Gram-stain-positive, catalase-negative, rod-shaped lactic acid bacterium	72:170	bacterium	162:170	A facultatively anaerobic, Gram-stain-positive, catalase-negative, rod-shaped lactic acid bacterium, designated SG816T, was isolated from small intestine of a swine.
30117800	11	14	theme	fatty	1210:1214	arg1	acids					1216:1220	The major cellular fatty acids	1191:1220	The major cellular fatty acids (>10 %) of the isolate	1191:1243	The major cellular fatty acids (>10 %) of the isolate were C18 : 1ω9c and C16 : 0.
30117800	11	14	theme	fatty	1210:1214	arg1	%					1227:1227	>10 %	1223:1227	>10 %	1223:1227	The major cellular fatty acids (>10 %) of the isolate were C18 : 1ω9c and C16 : 0.
30117800	11	14	theme	fatty	1210:1214	arg1	C18 					1250:1253	C18 	1250:1253	C18 	1250:1253	The major cellular fatty acids (>10 %) of the isolate were C18 : 1ω9c and C16 : 0.
30117800	5	15	theme	rRNA	401:404	arg1	sequencing					411:420	16S rRNA gene sequencing	397:420	16S rRNA gene sequencing	397:420	Comparative 16S rRNA gene sequencing and the matrix-associated laser desorption/ionization-time-of-flight mass spectometry profiling showed that strain SG816T was closely related to Lactobacillus delbrueckiisubsp.
30117800	5	16	theme	sequencing	411:420	arg1	profiling					508:516	Comparative 16S rRNA gene sequencing and the matrix-associated laser desorption/ionization-time-of-flight mass spectometry profiling	385:516	Comparative 16S rRNA gene sequencing and the matrix-associated laser desorption/ionization-time-of-flight mass spectometry profiling	385:516	Comparative 16S rRNA gene sequencing and the matrix-associated laser desorption/ionization-time-of-flight mass spectometry profiling showed that strain SG816T was closely related to Lactobacillus delbrueckiisubsp.
30117800	10	17	theme	d-lactic	1150:1157	arg1	acid					1159:1162	d-lactic acid	1150:1162	d-lactic acid from glucose fermentation	1150:1188	The strain was homofermentative and produced d-lactic acid from glucose fermentation.
30117800	2	18	theme	rod-shaped	139:148	arg1	bacterium					162:170	A facultatively anaerobic, Gram-stain-positive, catalase-negative, rod-shaped lactic acid bacterium	72:170	bacterium	162:170	A facultatively anaerobic, Gram-stain-positive, catalase-negative, rod-shaped lactic acid bacterium, designated SG816T, was isolated from small intestine of a swine.
30117800	12	19	theme	A4α	1301:1303	arg1	type					1292:1295	The peptidoglycan type	1274:1295	The peptidoglycan type	1274:1295	The peptidoglycan type was A4α l-Lys-d-Asp.
30117800	12	19	theme	A4α	1301:1303	arg1	l-Lys-d-Asp					1305:1315	A4α l-Lys-d-Asp	1301:1315	A4α l-Lys-d-Asp	1301:1315	The peptidoglycan type was A4α l-Lys-d-Asp.
30117800	13	20	theme	strain	1383:1388	arg1	SG816T					1390:1395	strain SG816T	1383:1395	strain SG816T	1383:1395	On the basis of distinct phenotypic and phylogenetic properties, strain SG816T represents a novel species of the genus Lactobacillus, for which the name Lactobacillus porci sp.
30117800	3	21	theme	Optimal	238:244	arg1	growth					246:251	Optimal growth	238:251	Optimal growth	238:251	Optimal growth occurred at 37 °C and pH 7.0.
30117800	15	22	theme	21090T=NBRC	1546:1556	arg1	112917T					1558:1564	=KCTC 21090T=NBRC 112917T	1540:1564	=KCTC 21090T=NBRC 112917T	1540:1564	The type strain is SG816T (=KCTC 21090T=NBRC 112917T).
30117800	15	22	theme	21090T=NBRC	1546:1556	arg1	SG816T					1532:1537	SG816T	1532:1537	SG816T (=KCTC 21090T=NBRC 112917T)	1532:1565	The type strain is SG816T (=KCTC 21090T=NBRC 112917T).
30117800	8	23	theme	housekeeping	836:847	arg1	subunit					929:935	phenylalanyl-tRNA synthase alpha subunit	896:935	phenylalanyl-tRNA synthase alpha subunit (pheS)	896:942	A comparison of two housekeeping genes, RNA polymerase alpha subunit (rpoA) and phenylalanyl-tRNA synthase alpha subunit (pheS), revealed that strain SG816T formed a separate branch within the clade of the genus Lactobacillus.
30117800	8	23	theme	housekeeping	836:847	arg1	genes					849:853	two housekeeping genes	832:853	two housekeeping genes	832:853	A comparison of two housekeeping genes, RNA polymerase alpha subunit (rpoA) and phenylalanyl-tRNA synthase alpha subunit (pheS), revealed that strain SG816T formed a separate branch within the clade of the genus Lactobacillus.
30117800	8	23	theme	housekeeping	836:847	arg1	subunit					877:883	RNA polymerase alpha subunit	856:883	RNA polymerase alpha subunit (rpoA)	856:890	A comparison of two housekeeping genes, RNA polymerase alpha subunit (rpoA) and phenylalanyl-tRNA synthase alpha subunit (pheS), revealed that strain SG816T formed a separate branch within the clade of the genus Lactobacillus.
30117800	2	24	attach	isolated	196:203	arg1	intestine					216:224	small intestine	210:224	small intestine of a swine	210:235	A facultatively anaerobic, Gram-stain-positive, catalase-negative, rod-shaped lactic acid bacterium, designated SG816T, was isolated from small intestine of a swine.
30117800	2	24	attach	isolated	196:203	arg2	bacterium					162:170	A facultatively anaerobic, Gram-stain-positive, catalase-negative, rod-shaped lactic acid bacterium	72:170	bacterium	162:170	A facultatively anaerobic, Gram-stain-positive, catalase-negative, rod-shaped lactic acid bacterium, designated SG816T, was isolated from small intestine of a swine.
30117800	0	25	theme	porci	14:18	arg1	sp					20:21	Lactobacillus porci sp	0:21	Lactobacillus porci sp.	0:22	Lactobacillus porci sp.
30117800	1	26	theme	swine	65:69	arg1	intestine					50:58	small intestine	44:58	small intestine of a swine	44:69	nov., isolated from small intestine of a swine.
30117800	5	27	theme	matrix-associated	430:446	arg1	mass					491:494	the matrix-associated laser desorption/ionization-time-of-flight mass	426:494	the matrix-associated laser desorption/ionization-time-of-flight mass	426:494	Comparative 16S rRNA gene sequencing and the matrix-associated laser desorption/ionization-time-of-flight mass spectometry profiling showed that strain SG816T was closely related to Lactobacillus delbrueckiisubsp.
30117800	0	28	theme	Lactobacillus	0:12	arg1	sp					20:21	Lactobacillus porci sp	0:21	Lactobacillus porci sp.	0:22	Lactobacillus porci sp.
30117800	13	29	theme	Lactobacillus	1471:1483	arg1	sp					1491:1492	the name Lactobacillus porci sp	1462:1492	the name Lactobacillus porci sp	1462:1492	On the basis of distinct phenotypic and phylogenetic properties, strain SG816T represents a novel species of the genus Lactobacillus, for which the name Lactobacillus porci sp.
30117800	13	30	theme	phenotypic	1343:1352	arg1	properties					1371:1380	distinct phenotypic and phylogenetic properties	1334:1380	distinct phenotypic and phylogenetic properties	1334:1380	On the basis of distinct phenotypic and phylogenetic properties, strain SG816T represents a novel species of the genus Lactobacillus, for which the name Lactobacillus porci sp.
30117800	8	31	theme	alpha	923:927	arg1	pheS					938:941	pheS	938:941	pheS	938:941	A comparison of two housekeeping genes, RNA polymerase alpha subunit (rpoA) and phenylalanyl-tRNA synthase alpha subunit (pheS), revealed that strain SG816T formed a separate branch within the clade of the genus Lactobacillus.
30117800	8	31	theme	alpha	923:927	arg1	subunit					929:935	phenylalanyl-tRNA synthase alpha subunit	896:935	phenylalanyl-tRNA synthase alpha subunit (pheS)	896:942	A comparison of two housekeeping genes, RNA polymerase alpha subunit (rpoA) and phenylalanyl-tRNA synthase alpha subunit (pheS), revealed that strain SG816T formed a separate branch within the clade of the genus Lactobacillus.
30117800	8	31	theme	alpha	923:927	arg1	genes					849:853	two housekeeping genes	832:853	two housekeeping genes	832:853	A comparison of two housekeeping genes, RNA polymerase alpha subunit (rpoA) and phenylalanyl-tRNA synthase alpha subunit (pheS), revealed that strain SG816T formed a separate branch within the clade of the genus Lactobacillus.
30117800	10	32	from	fermentation	1177:1188	arg1	acid					1159:1162	d-lactic acid	1150:1162	d-lactic acid from glucose fermentation	1150:1188	The strain was homofermentative and produced d-lactic acid from glucose fermentation.
30117800	5	33	theme	Comparative	385:395	arg1	profiling					508:516	Comparative 16S rRNA gene sequencing and the matrix-associated laser desorption/ionization-time-of-flight mass spectometry profiling	385:516	Comparative 16S rRNA gene sequencing and the matrix-associated laser desorption/ionization-time-of-flight mass spectometry profiling	385:516	Comparative 16S rRNA gene sequencing and the matrix-associated laser desorption/ionization-time-of-flight mass spectometry profiling showed that strain SG816T was closely related to Lactobacillus delbrueckiisubsp.
30117800	7	34	theme	equicursoris	782:793	arg1	19284T					799:804	other Lactobacillus delbrueckii subspecies (95.9-95.7 %) and Lactobacillus equicursoris DSM 19284T	707:804	other Lactobacillus delbrueckii subspecies (95.9-95.7 %) and Lactobacillus equicursoris DSM 19284T (95.6 %)	707:813	indicus JCM 15610T (95.9 %), followed by other Lactobacillus delbrueckii subspecies (95.9-95.7 %) and Lactobacillus equicursoris DSM 19284T (95.6 %).
30117800	7	34	theme	equicursoris	782:793	arg1	%					812:812	95.6 %	807:812	95.6 %	807:812	indicus JCM 15610T (95.9 %), followed by other Lactobacillus delbrueckii subspecies (95.9-95.7 %) and Lactobacillus equicursoris DSM 19284T (95.6 %).
30117800	15	35	theme	=KCTC	1540:1544	arg1	112917T					1558:1564	=KCTC 21090T=NBRC 112917T	1540:1564	=KCTC 21090T=NBRC 112917T	1540:1564	The type strain is SG816T (=KCTC 21090T=NBRC 112917T).
30117800	15	35	theme	=KCTC	1540:1544	arg1	SG816T					1532:1537	SG816T	1532:1537	SG816T (=KCTC 21090T=NBRC 112917T)	1532:1565	The type strain is SG816T (=KCTC 21090T=NBRC 112917T).
30117800	13	36	theme	name	1466:1469	arg1	sp					1491:1492	the name Lactobacillus porci sp	1462:1492	the name Lactobacillus porci sp	1462:1492	On the basis of distinct phenotypic and phylogenetic properties, strain SG816T represents a novel species of the genus Lactobacillus, for which the name Lactobacillus porci sp.
30117800	5	37	theme	16S	397:399	arg1	sequencing					411:420	16S rRNA gene sequencing	397:420	16S rRNA gene sequencing	397:420	Comparative 16S rRNA gene sequencing and the matrix-associated laser desorption/ionization-time-of-flight mass spectometry profiling showed that strain SG816T was closely related to Lactobacillus delbrueckiisubsp.
30117800	9	38	theme	SG816T	1083:1088	arg1	%					1102:1102	51.5 mol%	1094:1102	51.5 mol%	1094:1102	The DNA G+C content level of the strain SG816T was 51.5 mol%.
30117800	9	38	theme	SG816T	1083:1088	arg1	level					1063:1067	The DNA G+C content level	1043:1067	The DNA G+C content level of the strain SG816T	1043:1088	The DNA G+C content level of the strain SG816T was 51.5 mol%.
30117800	13	39	dep	properties	1371:1380	arg1	the					1321:1323	the	1321:1323	the	1321:1323	On the basis of distinct phenotypic and phylogenetic properties, strain SG816T represents a novel species of the genus Lactobacillus, for which the name Lactobacillus porci sp.
30117800	13	39	dep	properties	1371:1380	arg1	basis					1325:1329	basis	1325:1329	basis	1325:1329	On the basis of distinct phenotypic and phylogenetic properties, strain SG816T represents a novel species of the genus Lactobacillus, for which the name Lactobacillus porci sp.
30117800	6	40	theme	Lactobacillus	634:646	arg1	delbrueckiisubsp					648:663	Lactobacillus delbrueckiisubsp	634:663	Lactobacillus delbrueckiisubsp	634:663	bulgaricus KCTC 3635T (95.9 %) and Lactobacillus delbrueckiisubsp.
30117800	4	41	dep	NaCl	315:318	arg1	%					330:330	%	330:330	%	330:330	Furthermore, growth occurred in NaCl up to 0.5 % (w/v) but not at levels of salinity higher than 1 %.
30117800	13	42	theme	phylogenetic	1358:1369	arg1	properties					1371:1380	distinct phenotypic and phylogenetic properties	1334:1380	distinct phenotypic and phylogenetic properties	1334:1380	On the basis of distinct phenotypic and phylogenetic properties, strain SG816T represents a novel species of the genus Lactobacillus, for which the name Lactobacillus porci sp.
30117800	8	43	theme	genus	1022:1026	arg1	Lactobacillus					1028:1040	the genus Lactobacillus	1018:1040	the genus Lactobacillus	1018:1040	A comparison of two housekeeping genes, RNA polymerase alpha subunit (rpoA) and phenylalanyl-tRNA synthase alpha subunit (pheS), revealed that strain SG816T formed a separate branch within the clade of the genus Lactobacillus.
30117800	11	44	theme	major	1195:1199	arg1	acids					1216:1220	The major cellular fatty acids	1191:1220	The major cellular fatty acids (>10 %) of the isolate	1191:1243	The major cellular fatty acids (>10 %) of the isolate were C18 : 1ω9c and C16 : 0.
30117800	11	44	theme	major	1195:1199	arg1	%					1227:1227	>10 %	1223:1227	>10 %	1223:1227	The major cellular fatty acids (>10 %) of the isolate were C18 : 1ω9c and C16 : 0.
30117800	11	44	theme	major	1195:1199	arg1	C18 					1250:1253	C18 	1250:1253	C18 	1250:1253	The major cellular fatty acids (>10 %) of the isolate were C18 : 1ω9c and C16 : 0.
30117800	5	45	theme	gene	406:409	arg1	sequencing					411:420	16S rRNA gene sequencing	397:420	16S rRNA gene sequencing	397:420	Comparative 16S rRNA gene sequencing and the matrix-associated laser desorption/ionization-time-of-flight mass spectometry profiling showed that strain SG816T was closely related to Lactobacillus delbrueckiisubsp.
30117800	12	46	theme	peptidoglycan	1278:1290	arg1	type					1292:1295	The peptidoglycan type	1274:1295	The peptidoglycan type	1274:1295	The peptidoglycan type was A4α l-Lys-d-Asp.
30117800	12	46	theme	peptidoglycan	1278:1290	arg1	l-Lys-d-Asp					1305:1315	A4α l-Lys-d-Asp	1301:1315	A4α l-Lys-d-Asp	1301:1315	The peptidoglycan type was A4α l-Lys-d-Asp.
30117800	10	47	theme	glucose	1169:1175	arg1	fermentation					1177:1188	glucose fermentation	1169:1188	glucose fermentation	1169:1188	The strain was homofermentative and produced d-lactic acid from glucose fermentation.
30117800	7	48	theme	JCM	674:676	arg1	15610T					678:683	indicus JCM 15610T	666:683	indicus JCM 15610T (95.9 %)	666:692	indicus JCM 15610T (95.9 %), followed by other Lactobacillus delbrueckii subspecies (95.9-95.7 %) and Lactobacillus equicursoris DSM 19284T (95.6 %).
30117800	7	48	theme	JCM	674:676	arg1	%					691:691	95.9 %	686:691	95.9 %	686:691	indicus JCM 15610T (95.9 %), followed by other Lactobacillus delbrueckii subspecies (95.9-95.7 %) and Lactobacillus equicursoris DSM 19284T (95.6 %).
30117800	13	49	theme	Lactobacillus	1437:1449	arg1	species					1416:1422	a novel species	1408:1422	a novel species	1408:1422	On the basis of distinct phenotypic and phylogenetic properties, strain SG816T represents a novel species of the genus Lactobacillus, for which the name Lactobacillus porci sp.
30117800	9	50	theme	G+C content	1051:1061	arg1	%					1102:1102	51.5 mol%	1094:1102	51.5 mol%	1094:1102	The DNA G+C content level of the strain SG816T was 51.5 mol%.
30117800	9	50	theme	G+C content	1051:1061	arg1	level					1063:1067	The DNA G+C content level	1043:1067	The DNA G+C content level of the strain SG816T	1043:1088	The DNA G+C content level of the strain SG816T was 51.5 mol%.
30117800	2	51	dep	anaerobic	88:96	arg1	Gram-stain-positive					99:117	Gram-stain-positive	99:117	Gram-stain-positive	99:117	A facultatively anaerobic, Gram-stain-positive, catalase-negative, rod-shaped lactic acid bacterium, designated SG816T, was isolated from small intestine of a swine.
30117800	2	51	dep	anaerobic	88:96	arg1	catalase-negative					120:136	catalase-negative	120:136	catalase-negative	120:136	A facultatively anaerobic, Gram-stain-positive, catalase-negative, rod-shaped lactic acid bacterium, designated SG816T, was isolated from small intestine of a swine.
30117800	2	52	theme	acid	157:160	arg1	bacterium					162:170	A facultatively anaerobic, Gram-stain-positive, catalase-negative, rod-shaped lactic acid bacterium	72:170	bacterium	162:170	A facultatively anaerobic, Gram-stain-positive, catalase-negative, rod-shaped lactic acid bacterium, designated SG816T, was isolated from small intestine of a swine.
30117800	2	53	theme	small	210:214	arg1	intestine					216:224	small intestine	210:224	small intestine of a swine	210:235	A facultatively anaerobic, Gram-stain-positive, catalase-negative, rod-shaped lactic acid bacterium, designated SG816T, was isolated from small intestine of a swine.
30117800	8	54	theme	alpha	871:875	arg1	rpoA					886:889	rpoA	886:889	rpoA	886:889	A comparison of two housekeeping genes, RNA polymerase alpha subunit (rpoA) and phenylalanyl-tRNA synthase alpha subunit (pheS), revealed that strain SG816T formed a separate branch within the clade of the genus Lactobacillus.
30117800	8	54	theme	alpha	871:875	arg1	subunit					877:883	RNA polymerase alpha subunit	856:883	RNA polymerase alpha subunit (rpoA)	856:890	A comparison of two housekeeping genes, RNA polymerase alpha subunit (rpoA) and phenylalanyl-tRNA synthase alpha subunit (pheS), revealed that strain SG816T formed a separate branch within the clade of the genus Lactobacillus.
30117800	8	54	theme	alpha	871:875	arg1	genes					849:853	two housekeeping genes	832:853	two housekeeping genes	832:853	A comparison of two housekeeping genes, RNA polymerase alpha subunit (rpoA) and phenylalanyl-tRNA synthase alpha subunit (pheS), revealed that strain SG816T formed a separate branch within the clade of the genus Lactobacillus.
30117800	7	55	theme	subspecies	739:748	arg1	19284T					799:804	other Lactobacillus delbrueckii subspecies (95.9-95.7 %) and Lactobacillus equicursoris DSM 19284T	707:804	other Lactobacillus delbrueckii subspecies (95.9-95.7 %) and Lactobacillus equicursoris DSM 19284T (95.6 %)	707:813	indicus JCM 15610T (95.9 %), followed by other Lactobacillus delbrueckii subspecies (95.9-95.7 %) and Lactobacillus equicursoris DSM 19284T (95.6 %).
30117800	7	55	theme	subspecies	739:748	arg1	%					812:812	95.6 %	807:812	95.6 %	807:812	indicus JCM 15610T (95.9 %), followed by other Lactobacillus delbrueckii subspecies (95.9-95.7 %) and Lactobacillus equicursoris DSM 19284T (95.6 %).
30117800	8	56	theme	Lactobacillus	1028:1040	arg1	clade					1009:1013	the clade	1005:1013	the clade of the genus Lactobacillus	1005:1040	A comparison of two housekeeping genes, RNA polymerase alpha subunit (rpoA) and phenylalanyl-tRNA synthase alpha subunit (pheS), revealed that strain SG816T formed a separate branch within the clade of the genus Lactobacillus.
30117800	8	57	theme	separate	982:989	arg1	branch					991:996	a separate branch	980:996	a separate branch	980:996	A comparison of two housekeeping genes, RNA polymerase alpha subunit (rpoA) and phenylalanyl-tRNA synthase alpha subunit (pheS), revealed that strain SG816T formed a separate branch within the clade of the genus Lactobacillus.
30117800	15	58	theme	type	1517:1520	arg1	strain					1522:1527	The type strain	1513:1527	The type strain	1513:1527	The type strain is SG816T (=KCTC 21090T=NBRC 112917T).
30117800	15	58	theme	type	1517:1520	arg1	SG816T					1532:1537	SG816T	1532:1537	SG816T (=KCTC 21090T=NBRC 112917T)	1532:1565	The type strain is SG816T (=KCTC 21090T=NBRC 112917T).
30117800	7	59	theme	DSM	795:797	arg1	19284T					799:804	other Lactobacillus delbrueckii subspecies (95.9-95.7 %) and Lactobacillus equicursoris DSM 19284T	707:804	other Lactobacillus delbrueckii subspecies (95.9-95.7 %) and Lactobacillus equicursoris DSM 19284T (95.6 %)	707:813	indicus JCM 15610T (95.9 %), followed by other Lactobacillus delbrueckii subspecies (95.9-95.7 %) and Lactobacillus equicursoris DSM 19284T (95.6 %).
30117800	7	59	theme	DSM	795:797	arg1	%					812:812	95.6 %	807:812	95.6 %	807:812	indicus JCM 15610T (95.9 %), followed by other Lactobacillus delbrueckii subspecies (95.9-95.7 %) and Lactobacillus equicursoris DSM 19284T (95.6 %).
30117800	7	60	theme	indicus	666:672	arg1	15610T					678:683	indicus JCM 15610T	666:683	indicus JCM 15610T (95.9 %)	666:692	indicus JCM 15610T (95.9 %), followed by other Lactobacillus delbrueckii subspecies (95.9-95.7 %) and Lactobacillus equicursoris DSM 19284T (95.6 %).
30117800	7	60	theme	indicus	666:672	arg1	%					691:691	95.9 %	686:691	95.9 %	686:691	indicus JCM 15610T (95.9 %), followed by other Lactobacillus delbrueckii subspecies (95.9-95.7 %) and Lactobacillus equicursoris DSM 19284T (95.6 %).
30117800	13	61	theme	novel	1410:1414	arg1	species					1416:1422	a novel species	1408:1422	a novel species	1408:1422	On the basis of distinct phenotypic and phylogenetic properties, strain SG816T represents a novel species of the genus Lactobacillus, for which the name Lactobacillus porci sp.
30117800	5	62	theme	strain	530:535	arg1	SG816T					537:542	strain SG816T	530:542	strain SG816T	530:542	Comparative 16S rRNA gene sequencing and the matrix-associated laser desorption/ionization-time-of-flight mass spectometry profiling showed that strain SG816T was closely related to Lactobacillus delbrueckiisubsp.
30117800	13	63	theme	distinct	1334:1341	arg1	properties					1371:1380	distinct phenotypic and phylogenetic properties	1334:1380	distinct phenotypic and phylogenetic properties	1334:1380	On the basis of distinct phenotypic and phylogenetic properties, strain SG816T represents a novel species of the genus Lactobacillus, for which the name Lactobacillus porci sp.
30117800	8	64	theme	synthase	914:921	arg1	pheS					938:941	pheS	938:941	pheS	938:941	A comparison of two housekeeping genes, RNA polymerase alpha subunit (rpoA) and phenylalanyl-tRNA synthase alpha subunit (pheS), revealed that strain SG816T formed a separate branch within the clade of the genus Lactobacillus.
30117800	8	64	theme	synthase	914:921	arg1	subunit					929:935	phenylalanyl-tRNA synthase alpha subunit	896:935	phenylalanyl-tRNA synthase alpha subunit (pheS)	896:942	A comparison of two housekeeping genes, RNA polymerase alpha subunit (rpoA) and phenylalanyl-tRNA synthase alpha subunit (pheS), revealed that strain SG816T formed a separate branch within the clade of the genus Lactobacillus.
30117800	8	64	theme	synthase	914:921	arg1	genes					849:853	two housekeeping genes	832:853	two housekeeping genes	832:853	A comparison of two housekeeping genes, RNA polymerase alpha subunit (rpoA) and phenylalanyl-tRNA synthase alpha subunit (pheS), revealed that strain SG816T formed a separate branch within the clade of the genus Lactobacillus.
30117800	13	65	theme	genus	1431:1435	arg1	Lactobacillus					1437:1449	the genus Lactobacillus	1427:1449	the genus Lactobacillus	1427:1449	On the basis of distinct phenotypic and phylogenetic properties, strain SG816T represents a novel species of the genus Lactobacillus, for which the name Lactobacillus porci sp.
30117800	4	66	theme	higher	368:373	arg1	levels					349:354	levels	349:354	levels of salinity higher than 1 %	349:382	Furthermore, growth occurred in NaCl up to 0.5 % (w/v) but not at levels of salinity higher than 1 %.
30117800	13	67	theme	porci	1485:1489	arg1	sp					1491:1492	the name Lactobacillus porci sp	1462:1492	the name Lactobacillus porci sp	1462:1492	On the basis of distinct phenotypic and phylogenetic properties, strain SG816T represents a novel species of the genus Lactobacillus, for which the name Lactobacillus porci sp.
30117800	5	68	theme	laser	448:452	arg1	mass					491:494	the matrix-associated laser desorption/ionization-time-of-flight mass	426:494	the matrix-associated laser desorption/ionization-time-of-flight mass	426:494	Comparative 16S rRNA gene sequencing and the matrix-associated laser desorption/ionization-time-of-flight mass spectometry profiling showed that strain SG816T was closely related to Lactobacillus delbrueckiisubsp.
30117800	8	69	theme	genes	849:853	arg1	comparison					818:827	A comparison	816:827	A comparison of two housekeeping genes, RNA polymerase alpha subunit (rpoA) and phenylalanyl-tRNA synthase alpha subunit (pheS),	816:943	A comparison of two housekeeping genes, RNA polymerase alpha subunit (rpoA) and phenylalanyl-tRNA synthase alpha subunit (pheS), revealed that strain SG816T formed a separate branch within the clade of the genus Lactobacillus.
30117800	4	70	dep	%	330:330	arg1	0.5 					326:329	0.5 	326:329	0.5 	326:329	Furthermore, growth occurred in NaCl up to 0.5 % (w/v) but not at levels of salinity higher than 1 %.
30117800	4	70	dep	%	330:330	arg1	w/v					333:335	w/v	333:335	w/v	333:335	Furthermore, growth occurred in NaCl up to 0.5 % (w/v) but not at levels of salinity higher than 1 %.
30117800	4	71	theme	salinity	359:366	arg1	levels					349:354	levels	349:354	levels of salinity higher than 1 %	349:382	Furthermore, growth occurred in NaCl up to 0.5 % (w/v) but not at levels of salinity higher than 1 %.
30117800	2	72	theme	swine	231:235	arg1	intestine					216:224	small intestine	210:224	small intestine of a swine	210:235	A facultatively anaerobic, Gram-stain-positive, catalase-negative, rod-shaped lactic acid bacterium, designated SG816T, was isolated from small intestine of a swine.
30117800	5	73	theme	desorption/ionization-time-of-flight	454:489	arg1	mass					491:494	the matrix-associated laser desorption/ionization-time-of-flight mass	426:494	the matrix-associated laser desorption/ionization-time-of-flight mass	426:494	Comparative 16S rRNA gene sequencing and the matrix-associated laser desorption/ionization-time-of-flight mass spectometry profiling showed that strain SG816T was closely related to Lactobacillus delbrueckiisubsp.
30117800	4	74	dep	0.5 	326:329	arg1	to					323:324	to	323:324	to	323:324	Furthermore, growth occurred in NaCl up to 0.5 % (w/v) but not at levels of salinity higher than 1 %.
30117800	8	75	theme	RNA	856:858	arg1	rpoA					886:889	rpoA	886:889	rpoA	886:889	A comparison of two housekeeping genes, RNA polymerase alpha subunit (rpoA) and phenylalanyl-tRNA synthase alpha subunit (pheS), revealed that strain SG816T formed a separate branch within the clade of the genus Lactobacillus.
30117800	8	75	theme	RNA	856:858	arg1	subunit					877:883	RNA polymerase alpha subunit	856:883	RNA polymerase alpha subunit (rpoA)	856:890	A comparison of two housekeeping genes, RNA polymerase alpha subunit (rpoA) and phenylalanyl-tRNA synthase alpha subunit (pheS), revealed that strain SG816T formed a separate branch within the clade of the genus Lactobacillus.
30117800	8	75	theme	RNA	856:858	arg1	genes					849:853	two housekeeping genes	832:853	two housekeeping genes	832:853	A comparison of two housekeeping genes, RNA polymerase alpha subunit (rpoA) and phenylalanyl-tRNA synthase alpha subunit (pheS), revealed that strain SG816T formed a separate branch within the clade of the genus Lactobacillus.
30117800	11	76	theme	isolate	1237:1243	arg1	acids					1216:1220	The major cellular fatty acids	1191:1220	The major cellular fatty acids (>10 %) of the isolate	1191:1243	The major cellular fatty acids (>10 %) of the isolate were C18 : 1ω9c and C16 : 0.
30117800	11	76	theme	isolate	1237:1243	arg1	%					1227:1227	>10 %	1223:1227	>10 %	1223:1227	The major cellular fatty acids (>10 %) of the isolate were C18 : 1ω9c and C16 : 0.
30117800	11	76	theme	isolate	1237:1243	arg1	C18 					1250:1253	C18 	1250:1253	C18 	1250:1253	The major cellular fatty acids (>10 %) of the isolate were C18 : 1ω9c and C16 : 0.
30117800	5	77	theme	mass	491:494	arg1	profiling					508:516	Comparative 16S rRNA gene sequencing and the matrix-associated laser desorption/ionization-time-of-flight mass spectometry profiling	385:516	Comparative 16S rRNA gene sequencing and the matrix-associated laser desorption/ionization-time-of-flight mass spectometry profiling	385:516	Comparative 16S rRNA gene sequencing and the matrix-associated laser desorption/ionization-time-of-flight mass spectometry profiling showed that strain SG816T was closely related to Lactobacillus delbrueckiisubsp.
30117800	7	78	theme	other	707:711	arg1	19284T					799:804	other Lactobacillus delbrueckii subspecies (95.9-95.7 %) and Lactobacillus equicursoris DSM 19284T	707:804	other Lactobacillus delbrueckii subspecies (95.9-95.7 %) and Lactobacillus equicursoris DSM 19284T (95.6 %)	707:813	indicus JCM 15610T (95.9 %), followed by other Lactobacillus delbrueckii subspecies (95.9-95.7 %) and Lactobacillus equicursoris DSM 19284T (95.6 %).
30117800	7	78	theme	other	707:711	arg1	%					812:812	95.6 %	807:812	95.6 %	807:812	indicus JCM 15610T (95.9 %), followed by other Lactobacillus delbrueckii subspecies (95.9-95.7 %) and Lactobacillus equicursoris DSM 19284T (95.6 %).
29748817	10	0	from	effects	1746:1752	arg1	health					1763:1768	human health	1757:1768	human health	1757:1768	This calls for further coordinated state-of-the art research to better understand and document the human gut microbiome's effects on human health.
29748817	8	1	theme	mechanisms	1394:1403	arg1	degree					1310:1315	the degree	1306:1315	the degree of scientific evidence, the models and technologies	1306:1367	The gut microbiome affects virtually all aspects of human health, but the degree of scientific evidence, the models and technologies and the understanding of mechanisms of action vary considerably from one benefit area to the other.
29748817	8	1	theme	mechanisms	1394:1403	arg1	understanding					1377:1389	the understanding	1373:1389	the understanding of mechanisms of action	1373:1413	The gut microbiome affects virtually all aspects of human health, but the degree of scientific evidence, the models and technologies and the understanding of mechanisms of action vary considerably from one benefit area to the other.
29748817	4	2	theme	immune	825:830	arg1	response					832:839	the immune response	821:839	the immune response	821:839	Microbial metabolites, e.g. short-chain fatty acids affect gut-brain signaling and the immune response.
29748817	7	3	theme	microbiota	1129:1138	arg1	FMT					1157:1159	FMT	1157:1159	FMT	1157:1159	Fecal microbiota transplantation (FMT) highly effective for treating recurrent Clostridium difficile infections.
29748817	7	3	theme	microbiota	1129:1138	arg1	transplantation					1140:1154	Fecal microbiota transplantation	1123:1154	Fecal microbiota transplantation (FMT)	1123:1160	Fecal microbiota transplantation (FMT) highly effective for treating recurrent Clostridium difficile infections.
29748817	5	4	theme	gut-brain	945:953	arg1	axis					955:958	the gut-brain axis	941:958	the gut-brain axis	941:958	The gut microbiota has a regulatory role on anxiety, mood, cognition and pain which is exerted via the gut-brain axis.
29748817	1	5	theme	gut	215:217	arg1	microbiome					219:228	the gut microbiome	211:228	the gut microbiome	211:228	The 2017 annual symposium organized by the University Medical Center Groningen in The Netherlands focused on the role of the gut microbiome in human health and disease.
29748817	9	6	theme	side	1575:1578	arg1	effects					1580:1586	potential side effects	1565:1586	potential side effects	1565:1586	For a clinical practice to be broadly accepted, the mode of action, the therapeutic window, and potential side effects need to thoroughly be investigated.
29748817	3	7	theme	extrinsic	616:624	arg1	factors					626:632	extrinsic factors	616:632	extrinsic factors (such as diet, and drugs)	616:658	The gut microbiota changes dramatically during pregnancy and intrinsic factors (such as stress), in addition to extrinsic factors (such as diet, and drugs) influence the composition and activity of the gut microbiome throughout life.
29748817	9	8	theme	action	1529:1534	arg1	mode					1521:1524	the mode	1517:1524	the mode of action, the therapeutic window, and potential side effects	1517:1586	For a clinical practice to be broadly accepted, the mode of action, the therapeutic window, and potential side effects need to thoroughly be investigated.
29748817	1	9	theme	University	133:142	arg1	Center					152:157	the University Medical Center Groningen in The Netherlands focused on the role of the gut microbiome in human health and disease	129:256	the University Medical Center Groningen in The Netherlands focused on the role of the gut microbiome in human health and disease	129:256	The 2017 annual symposium organized by the University Medical Center Groningen in The Netherlands focused on the role of the gut microbiome in human health and disease.
29748817	8	10	theme	human	1288:1292	arg1	health					1294:1299	human health	1288:1299	human health	1288:1299	The gut microbiome affects virtually all aspects of human health, but the degree of scientific evidence, the models and technologies and the understanding of mechanisms of action vary considerably from one benefit area to the other.
29748817	2	11	from	early-life	439:448	arg1	microbiome					368:377	the gut microbiome	360:377	the gut microbiome in health and disease	360:399	Experts from academia and industry examined interactions of prebiotics, probiotics, or vitamins with the gut microbiome in health and disease, the development of the microbiome in early-life and the role of the microbiome on the gut-brain axis.
29748817	2	11	from	early-life	439:448	arg1	role					458:461	the role	454:461	the role of the microbiome on the gut-brain axis	454:501	Experts from academia and industry examined interactions of prebiotics, probiotics, or vitamins with the gut microbiome in health and disease, the development of the microbiome in early-life and the role of the microbiome on the gut-brain axis.
29748817	2	11	from	early-life	439:448	arg1	development					406:416	the development	402:416	the development of the microbiome in early-life	402:448	Experts from academia and industry examined interactions of prebiotics, probiotics, or vitamins with the gut microbiome in health and disease, the development of the microbiome in early-life and the role of the microbiome on the gut-brain axis.
29748817	0	12	theme	clinical	67:74	arg1	applications					76:87	clinical applications	67:87	clinical applications	67:87	The role of the microbiome for human health: from basic science to clinical applications.
29748817	8	13	theme	action	1408:1413	arg1	mechanisms					1394:1403	mechanisms	1394:1403	mechanisms of action	1394:1413	The gut microbiome affects virtually all aspects of human health, but the degree of scientific evidence, the models and technologies and the understanding of mechanisms of action vary considerably from one benefit area to the other.
29748817	2	14	from	industry	285:292	arg1	Experts					259:265	Experts	259:265	Experts from academia and industry	259:292	Experts from academia and industry examined interactions of prebiotics, probiotics, or vitamins with the gut microbiome in health and disease, the development of the microbiome in early-life and the role of the microbiome on the gut-brain axis.
29748817	2	15	from	academia	272:279	arg1	Experts					259:265	Experts	259:265	Experts from academia and industry	259:292	Experts from academia and industry examined interactions of prebiotics, probiotics, or vitamins with the gut microbiome in health and disease, the development of the microbiome in early-life and the role of the microbiome on the gut-brain axis.
29748817	10	16	theme	art	1672:1674	arg1	research					1676:1683	further coordinated state-of-the art research	1639:1683	further coordinated state-of-the art research	1639:1683	This calls for further coordinated state-of-the art research to better understand and document the human gut microbiome's effects on human health.
29748817	3	17	dep	composition	674:684	arg1	the					670:672	the	670:672	the	670:672	The gut microbiota changes dramatically during pregnancy and intrinsic factors (such as stress), in addition to extrinsic factors (such as diet, and drugs) influence the composition and activity of the gut microbiome throughout life.
29748817	4	18	theme	gut-brain	797:805	arg1	signaling					807:815	gut-brain signaling	797:815	gut-brain signaling	797:815	Microbial metabolites, e.g. short-chain fatty acids affect gut-brain signaling and the immune response.
29748817	8	19	theme	gut	1240:1242	arg1	microbiome					1244:1253	The gut microbiome	1236:1253	The gut microbiome	1236:1253	The gut microbiome affects virtually all aspects of human health, but the degree of scientific evidence, the models and technologies and the understanding of mechanisms of action vary considerably from one benefit area to the other.
29748817	1	20	theme	human	233:237	arg1	health					239:244	human health	233:244	human health	233:244	The 2017 annual symposium organized by the University Medical Center Groningen in The Netherlands focused on the role of the gut microbiome in human health and disease.
29748817	2	21	from	development	406:416	arg1	early-life					439:448	early-life	439:448	early-life	439:448	Experts from academia and industry examined interactions of prebiotics, probiotics, or vitamins with the gut microbiome in health and disease, the development of the microbiome in early-life and the role of the microbiome on the gut-brain axis.
29748817	2	21	from	development	406:416	arg1	axis					498:501	the gut-brain axis	484:501	the gut-brain axis	484:501	Experts from academia and industry examined interactions of prebiotics, probiotics, or vitamins with the gut microbiome in health and disease, the development of the microbiome in early-life and the role of the microbiome on the gut-brain axis.
29748817	2	21	from	development	406:416	arg1	disease					393:399	disease	393:399	disease	393:399	Experts from academia and industry examined interactions of prebiotics, probiotics, or vitamins with the gut microbiome in health and disease, the development of the microbiome in early-life and the role of the microbiome on the gut-brain axis.
29748817	2	21	from	development	406:416	arg1	health					382:387	health	382:387	health	382:387	Experts from academia and industry examined interactions of prebiotics, probiotics, or vitamins with the gut microbiome in health and disease, the development of the microbiome in early-life and the role of the microbiome on the gut-brain axis.
29748817	9	22	theme	therapeutic	1541:1551	arg1	window					1553:1558	the therapeutic window	1537:1558	the therapeutic window	1537:1558	For a clinical practice to be broadly accepted, the mode of action, the therapeutic window, and potential side effects need to thoroughly be investigated.
29748817	6	23	from	infections	1091:1100	arg1	infancy					1105:1111	infancy	1105:1111	infancy	1105:1111	Ingestion of prebiotics or probiotics has been used to treat a range of conditions including constipation, allergic reactions and infections in infancy, and IBS.
29748817	6	24	theme	allergic	1068:1075	arg1	reactions					1077:1085	allergic reactions	1068:1085	allergic reactions	1068:1085	Ingestion of prebiotics or probiotics has been used to treat a range of conditions including constipation, allergic reactions and infections in infancy, and IBS.
29748817	1	25	theme	Medical	144:150	arg1	Center					152:157	the University Medical Center Groningen in The Netherlands focused on the role of the gut microbiome in human health and disease	129:256	the University Medical Center Groningen in The Netherlands focused on the role of the gut microbiome in human health and disease	129:256	The 2017 annual symposium organized by the University Medical Center Groningen in The Netherlands focused on the role of the gut microbiome in human health and disease.
29748817	4	26	dep	acids	784:788	arg1	e.g.					761:764	e.g.	761:764	e.g.	761:764	Microbial metabolites, e.g. short-chain fatty acids affect gut-brain signaling and the immune response.
29748817	2	27	from	microbiome	425:434	arg1	early-life					439:448	early-life	439:448	early-life	439:448	Experts from academia and industry examined interactions of prebiotics, probiotics, or vitamins with the gut microbiome in health and disease, the development of the microbiome in early-life and the role of the microbiome on the gut-brain axis.
29748817	9	28	theme	window	1553:1558	arg1	mode					1521:1524	the mode	1517:1524	the mode of action, the therapeutic window, and potential side effects	1517:1586	For a clinical practice to be broadly accepted, the mode of action, the therapeutic window, and potential side effects need to thoroughly be investigated.
29748817	6	29	theme	probiotics	988:997	arg1	Ingestion					961:969	Ingestion	961:969	Ingestion of prebiotics or probiotics	961:997	Ingestion of prebiotics or probiotics has been used to treat a range of conditions including constipation, allergic reactions and infections in infancy, and IBS.
29748817	6	30	theme	prebiotics	974:983	arg1	Ingestion					961:969	Ingestion	961:969	Ingestion of prebiotics or probiotics	961:997	Ingestion of prebiotics or probiotics has been used to treat a range of conditions including constipation, allergic reactions and infections in infancy, and IBS.
29748817	4	31	theme	fatty	778:782	arg1	acids					784:788	short-chain fatty acids	766:788	short-chain fatty acids	766:788	Microbial metabolites, e.g. short-chain fatty acids affect gut-brain signaling and the immune response.
29748817	3	32	theme	gut	508:510	arg1	microbiota					512:521	The gut microbiota	504:521	The gut microbiota	504:521	The gut microbiota changes dramatically during pregnancy and intrinsic factors (such as stress), in addition to extrinsic factors (such as diet, and drugs) influence the composition and activity of the gut microbiome throughout life.
29748817	6	33	from	reactions	1077:1085	arg1	infancy					1105:1111	infancy	1105:1111	infancy	1105:1111	Ingestion of prebiotics or probiotics has been used to treat a range of conditions including constipation, allergic reactions and infections in infancy, and IBS.
29748817	2	34	theme	vitamins	346:353	arg1	interactions					303:314	interactions	303:314	interactions of prebiotics, probiotics, or vitamins with the gut microbiome in health and disease, the development of the microbiome in early-life and the role of the microbiome on the gut-brain axis	303:501	Experts from academia and industry examined interactions of prebiotics, probiotics, or vitamins with the gut microbiome in health and disease, the development of the microbiome in early-life and the role of the microbiome on the gut-brain axis.
29748817	3	35	dep	influence	660:668	arg1	addition					604:611	addition	604:611	addition	604:611	The gut microbiota changes dramatically during pregnancy and intrinsic factors (such as stress), in addition to extrinsic factors (such as diet, and drugs) influence the composition and activity of the gut microbiome throughout life.
29748817	4	36	theme	short-chain	766:776	arg1	acids					784:788	short-chain fatty acids	766:788	short-chain fatty acids	766:788	Microbial metabolites, e.g. short-chain fatty acids affect gut-brain signaling and the immune response.
29748817	10	37	theme	state-of-the	1659:1670	arg1	research					1676:1683	further coordinated state-of-the art research	1639:1683	further coordinated state-of-the art research	1639:1683	This calls for further coordinated state-of-the art research to better understand and document the human gut microbiome's effects on human health.
29748817	8	38	theme	all	1273:1275	arg1	aspects					1277:1283	virtually all aspects	1263:1283	virtually all aspects of human health	1263:1299	The gut microbiome affects virtually all aspects of human health, but the degree of scientific evidence, the models and technologies and the understanding of mechanisms of action vary considerably from one benefit area to the other.
29748817	3	39	dep	factors	626:632	arg1	such					635:638	such	635:638	such	635:638	The gut microbiota changes dramatically during pregnancy and intrinsic factors (such as stress), in addition to extrinsic factors (such as diet, and drugs) influence the composition and activity of the gut microbiome throughout life.
29748817	9	40	theme	potential	1565:1573	arg1	effects					1580:1586	potential side effects	1565:1586	potential side effects	1565:1586	For a clinical practice to be broadly accepted, the mode of action, the therapeutic window, and potential side effects need to thoroughly be investigated.
29748817	4	41	theme	Microbial	738:746	arg1	metabolites					748:758	Microbial metabolites	738:758	Microbial metabolites	738:758	Microbial metabolites, e.g. short-chain fatty acids affect gut-brain signaling and the immune response.
29748817	5	42	theme	regulatory	867:876	arg1	role					878:881	a regulatory role	865:881	a regulatory role	865:881	The gut microbiota has a regulatory role on anxiety, mood, cognition and pain which is exerted via the gut-brain axis.
29748817	7	43	theme	Clostridium	1202:1212	arg1	infections					1224:1233	recurrent Clostridium difficile infections	1192:1233	recurrent Clostridium difficile infections	1192:1233	Fecal microbiota transplantation (FMT) highly effective for treating recurrent Clostridium difficile infections.
29748817	2	44	theme	gut-brain	488:496	arg1	axis					498:501	the gut-brain axis	484:501	the gut-brain axis	484:501	Experts from academia and industry examined interactions of prebiotics, probiotics, or vitamins with the gut microbiome in health and disease, the development of the microbiome in early-life and the role of the microbiome on the gut-brain axis.
29748817	0	45	from	science	56:62	arg1	health					37:42	human health:	31:43	human health: from basic science to clinical applications	31:87	The role of the microbiome for human health: from basic science to clinical applications.
29748817	2	46	theme	prebiotics	319:328	arg1	interactions					303:314	interactions	303:314	interactions of prebiotics, probiotics, or vitamins with the gut microbiome in health and disease, the development of the microbiome in early-life and the role of the microbiome on the gut-brain axis	303:501	Experts from academia and industry examined interactions of prebiotics, probiotics, or vitamins with the gut microbiome in health and disease, the development of the microbiome in early-life and the role of the microbiome on the gut-brain axis.
29748817	6	47	from	constipation	1054:1065	arg1	infancy					1105:1111	infancy	1105:1111	infancy	1105:1111	Ingestion of prebiotics or probiotics has been used to treat a range of conditions including constipation, allergic reactions and infections in infancy, and IBS.
29748817	7	48	theme	difficile	1214:1222	arg1	infections					1224:1233	recurrent Clostridium difficile infections	1192:1233	recurrent Clostridium difficile infections	1192:1233	Fecal microbiota transplantation (FMT) highly effective for treating recurrent Clostridium difficile infections.
29748817	2	49	theme	microbiome	425:434	arg1	microbiome					368:377	the gut microbiome	360:377	the gut microbiome in health and disease	360:399	Experts from academia and industry examined interactions of prebiotics, probiotics, or vitamins with the gut microbiome in health and disease, the development of the microbiome in early-life and the role of the microbiome on the gut-brain axis.
29748817	2	49	theme	microbiome	425:434	arg1	role					458:461	the role	454:461	the role of the microbiome on the gut-brain axis	454:501	Experts from academia and industry examined interactions of prebiotics, probiotics, or vitamins with the gut microbiome in health and disease, the development of the microbiome in early-life and the role of the microbiome on the gut-brain axis.
29748817	2	49	theme	microbiome	425:434	arg1	development					406:416	the development	402:416	the development of the microbiome in early-life	402:448	Experts from academia and industry examined interactions of prebiotics, probiotics, or vitamins with the gut microbiome in health and disease, the development of the microbiome in early-life and the role of the microbiome on the gut-brain axis.
29748817	10	50	theme	coordinated	1647:1657	arg1	research					1676:1683	further coordinated state-of-the art research	1639:1683	further coordinated state-of-the art research	1639:1683	This calls for further coordinated state-of-the art research to better understand and document the human gut microbiome's effects on human health.
29748817	9	51	theme	effects	1580:1586	arg1	mode					1521:1524	the mode	1517:1524	the mode of action, the therapeutic window, and potential side effects	1517:1586	For a clinical practice to be broadly accepted, the mode of action, the therapeutic window, and potential side effects need to thoroughly be investigated.
29748817	5	52	contain	has	861:863	arg2	role					878:881	a regulatory role	865:881	a regulatory role	865:881	The gut microbiota has a regulatory role on anxiety, mood, cognition and pain which is exerted via the gut-brain axis.
29748817	5	52	contain	has	861:863	arg1	microbiota					850:859	The gut microbiota	842:859	The gut microbiota	842:859	The gut microbiota has a regulatory role on anxiety, mood, cognition and pain which is exerted via the gut-brain axis.
29748817	3	53	dep	factors	575:581	arg1	such					584:587	such	584:587	such	584:587	The gut microbiota changes dramatically during pregnancy and intrinsic factors (such as stress), in addition to extrinsic factors (such as diet, and drugs) influence the composition and activity of the gut microbiome throughout life.
29748817	0	54	theme	microbiome	16:25	arg1	role					4:7	The role	0:7	The role of the microbiome for human health: from basic science to clinical applications.	0:88	The role of the microbiome for human health: from basic science to clinical applications.
29748817	1	55	theme	microbiome	219:228	arg1	role					203:206	the role	199:206	the role of the gut microbiome in human health and disease	199:256	The 2017 annual symposium organized by the University Medical Center Groningen in The Netherlands focused on the role of the gut microbiome in human health and disease.
29748817	2	56	theme	gut	364:366	arg1	microbiome					368:377	the gut microbiome	360:377	the gut microbiome in health and disease	360:399	Experts from academia and industry examined interactions of prebiotics, probiotics, or vitamins with the gut microbiome in health and disease, the development of the microbiome in early-life and the role of the microbiome on the gut-brain axis.
29748817	2	57	theme	microbiome	470:479	arg1	microbiome					368:377	the gut microbiome	360:377	the gut microbiome in health and disease	360:399	Experts from academia and industry examined interactions of prebiotics, probiotics, or vitamins with the gut microbiome in health and disease, the development of the microbiome in early-life and the role of the microbiome on the gut-brain axis.
29748817	2	57	theme	microbiome	470:479	arg1	role					458:461	the role	454:461	the role of the microbiome on the gut-brain axis	454:501	Experts from academia and industry examined interactions of prebiotics, probiotics, or vitamins with the gut microbiome in health and disease, the development of the microbiome in early-life and the role of the microbiome on the gut-brain axis.
29748817	2	57	theme	microbiome	470:479	arg1	development					406:416	the development	402:416	the development of the microbiome in early-life	402:448	Experts from academia and industry examined interactions of prebiotics, probiotics, or vitamins with the gut microbiome in health and disease, the development of the microbiome in early-life and the role of the microbiome on the gut-brain axis.
29748817	2	58	with	interactions	303:314	arg1	microbiome					368:377	the gut microbiome	360:377	the gut microbiome in health and disease	360:399	Experts from academia and industry examined interactions of prebiotics, probiotics, or vitamins with the gut microbiome in health and disease, the development of the microbiome in early-life and the role of the microbiome on the gut-brain axis.
29748817	2	58	with	interactions	303:314	arg1	development					406:416	the development	402:416	the development of the microbiome in early-life	402:448	Experts from academia and industry examined interactions of prebiotics, probiotics, or vitamins with the gut microbiome in health and disease, the development of the microbiome in early-life and the role of the microbiome on the gut-brain axis.
29748817	2	58	with	interactions	303:314	arg1	role					458:461	the role	454:461	the role of the microbiome on the gut-brain axis	454:501	Experts from academia and industry examined interactions of prebiotics, probiotics, or vitamins with the gut microbiome in health and disease, the development of the microbiome in early-life and the role of the microbiome on the gut-brain axis.
29748817	8	59	theme	benefit	1442:1448	arg1	area					1450:1453	one benefit area	1438:1453	one benefit area	1438:1453	The gut microbiome affects virtually all aspects of human health, but the degree of scientific evidence, the models and technologies and the understanding of mechanisms of action vary considerably from one benefit area to the other.
29748817	0	60	theme	human	31:35	arg1	health					37:42	human health:	31:43	human health: from basic science to clinical applications	31:87	The role of the microbiome for human health: from basic science to clinical applications.
29748817	10	61	theme	human	1723:1727	arg1	microbiome					1733:1742	human gut microbiome's	1723:1744	the human gut microbiome's effects on human health	1719:1768	This calls for further coordinated state-of-the art research to better understand and document the human gut microbiome's effects on human health.
29748817	1	62	dep	Center	152:157	arg1	Groningen					159:167	Groningen	159:167	Groningen	159:167	The 2017 annual symposium organized by the University Medical Center Groningen in The Netherlands focused on the role of the gut microbiome in human health and disease.
29748817	6	63	from	IBS	1118:1120	arg1	infancy					1105:1111	infancy	1105:1111	infancy	1105:1111	Ingestion of prebiotics or probiotics has been used to treat a range of conditions including constipation, allergic reactions and infections in infancy, and IBS.
29748817	8	64	theme	evidence	1331:1338	arg1	degree					1310:1315	the degree	1306:1315	the degree of scientific evidence, the models and technologies	1306:1367	The gut microbiome affects virtually all aspects of human health, but the degree of scientific evidence, the models and technologies and the understanding of mechanisms of action vary considerably from one benefit area to the other.
29748817	8	64	theme	evidence	1331:1338	arg1	understanding					1377:1389	the understanding	1373:1389	the understanding of mechanisms of action	1373:1413	The gut microbiome affects virtually all aspects of human health, but the degree of scientific evidence, the models and technologies and the understanding of mechanisms of action vary considerably from one benefit area to the other.
29748817	2	65	from	role	458:461	arg1	early-life					439:448	early-life	439:448	early-life	439:448	Experts from academia and industry examined interactions of prebiotics, probiotics, or vitamins with the gut microbiome in health and disease, the development of the microbiome in early-life and the role of the microbiome on the gut-brain axis.
29748817	2	65	from	role	458:461	arg1	axis					498:501	the gut-brain axis	484:501	the gut-brain axis	484:501	Experts from academia and industry examined interactions of prebiotics, probiotics, or vitamins with the gut microbiome in health and disease, the development of the microbiome in early-life and the role of the microbiome on the gut-brain axis.
29748817	2	65	from	role	458:461	arg1	disease					393:399	disease	393:399	disease	393:399	Experts from academia and industry examined interactions of prebiotics, probiotics, or vitamins with the gut microbiome in health and disease, the development of the microbiome in early-life and the role of the microbiome on the gut-brain axis.
29748817	2	65	from	role	458:461	arg1	health					382:387	health	382:387	health	382:387	Experts from academia and industry examined interactions of prebiotics, probiotics, or vitamins with the gut microbiome in health and disease, the development of the microbiome in early-life and the role of the microbiome on the gut-brain axis.
29748817	8	66	theme	scientific	1320:1329	arg1	technologies					1356:1367	technologies	1356:1367	technologies	1356:1367	The gut microbiome affects virtually all aspects of human health, but the degree of scientific evidence, the models and technologies and the understanding of mechanisms of action vary considerably from one benefit area to the other.
29748817	8	66	theme	scientific	1320:1329	arg1	evidence					1331:1338	scientific evidence	1320:1338	scientific evidence	1320:1338	The gut microbiome affects virtually all aspects of human health, but the degree of scientific evidence, the models and technologies and the understanding of mechanisms of action vary considerably from one benefit area to the other.
29748817	8	66	theme	scientific	1320:1329	arg1	models					1345:1350	models	1345:1350	models	1345:1350	The gut microbiome affects virtually all aspects of human health, but the degree of scientific evidence, the models and technologies and the understanding of mechanisms of action vary considerably from one benefit area to the other.
29748817	6	67	used	used	1008:1011	arg2	Ingestion					961:969	Ingestion	961:969	Ingestion of prebiotics or probiotics	961:997	Ingestion of prebiotics or probiotics has been used to treat a range of conditions including constipation, allergic reactions and infections in infancy, and IBS.
29748817	1	68	theme	annual	99:104	arg1	symposium					106:114	The 2017 annual symposium	90:114	The 2017 annual symposium	90:114	The 2017 annual symposium organized by the University Medical Center Groningen in The Netherlands focused on the role of the gut microbiome in human health and disease.
29748817	6	69	theme	conditions	1033:1042	arg1	IBS					1118:1120	IBS	1118:1120	IBS	1118:1120	Ingestion of prebiotics or probiotics has been used to treat a range of conditions including constipation, allergic reactions and infections in infancy, and IBS.
29748817	6	69	theme	conditions	1033:1042	arg1	constipation					1054:1065	constipation	1054:1065	constipation	1054:1065	Ingestion of prebiotics or probiotics has been used to treat a range of conditions including constipation, allergic reactions and infections in infancy, and IBS.
29748817	6	69	theme	conditions	1033:1042	arg1	reactions					1077:1085	allergic reactions	1068:1085	allergic reactions	1068:1085	Ingestion of prebiotics or probiotics has been used to treat a range of conditions including constipation, allergic reactions and infections in infancy, and IBS.
29748817	6	69	theme	conditions	1033:1042	arg1	range					1024:1028	a range	1022:1028	a range of conditions including constipation, allergic reactions and infections in infancy, and IBS	1022:1120	Ingestion of prebiotics or probiotics has been used to treat a range of conditions including constipation, allergic reactions and infections in infancy, and IBS.
29748817	3	70	theme	microbiome	710:719	arg1	activity					690:697	activity	690:697	activity	690:697	The gut microbiota changes dramatically during pregnancy and intrinsic factors (such as stress), in addition to extrinsic factors (such as diet, and drugs) influence the composition and activity of the gut microbiome throughout life.
29748817	3	70	theme	microbiome	710:719	arg1	composition					674:684	composition	674:684	composition	674:684	The gut microbiota changes dramatically during pregnancy and intrinsic factors (such as stress), in addition to extrinsic factors (such as diet, and drugs) influence the composition and activity of the gut microbiome throughout life.
29748817	1	71	from	role	203:206	arg1	disease					250:256	disease	250:256	disease	250:256	The 2017 annual symposium organized by the University Medical Center Groningen in The Netherlands focused on the role of the gut microbiome in human health and disease.
29748817	1	71	from	role	203:206	arg1	health					239:244	human health	233:244	human health	233:244	The 2017 annual symposium organized by the University Medical Center Groningen in The Netherlands focused on the role of the gut microbiome in human health and disease.
29748817	10	72	theme	human	1757:1761	arg1	health					1763:1768	human health	1757:1768	human health	1757:1768	This calls for further coordinated state-of-the art research to better understand and document the human gut microbiome's effects on human health.
29748817	2	73	from	microbiome	368:377	arg1	early-life					439:448	early-life	439:448	early-life	439:448	Experts from academia and industry examined interactions of prebiotics, probiotics, or vitamins with the gut microbiome in health and disease, the development of the microbiome in early-life and the role of the microbiome on the gut-brain axis.
29748817	2	73	from	microbiome	368:377	arg1	axis					498:501	the gut-brain axis	484:501	the gut-brain axis	484:501	Experts from academia and industry examined interactions of prebiotics, probiotics, or vitamins with the gut microbiome in health and disease, the development of the microbiome in early-life and the role of the microbiome on the gut-brain axis.
29748817	2	73	from	microbiome	368:377	arg1	disease					393:399	disease	393:399	disease	393:399	Experts from academia and industry examined interactions of prebiotics, probiotics, or vitamins with the gut microbiome in health and disease, the development of the microbiome in early-life and the role of the microbiome on the gut-brain axis.
29748817	2	73	from	microbiome	368:377	arg1	health					382:387	health	382:387	health	382:387	Experts from academia and industry examined interactions of prebiotics, probiotics, or vitamins with the gut microbiome in health and disease, the development of the microbiome in early-life and the role of the microbiome on the gut-brain axis.
29748817	5	74	theme	gut	846:848	arg1	microbiota					850:859	The gut microbiota	842:859	The gut microbiota	842:859	The gut microbiota has a regulatory role on anxiety, mood, cognition and pain which is exerted via the gut-brain axis.
29748817	7	75	dep	effective	1169:1177	arg1	FMT					1157:1159	FMT	1157:1159	FMT	1157:1159	Fecal microbiota transplantation (FMT) highly effective for treating recurrent Clostridium difficile infections.
29748817	7	75	dep	effective	1169:1177	arg1	transplantation					1140:1154	Fecal microbiota transplantation	1123:1154	Fecal microbiota transplantation (FMT)	1123:1160	Fecal microbiota transplantation (FMT) highly effective for treating recurrent Clostridium difficile infections.
29748817	7	76	theme	recurrent	1192:1200	arg1	infections					1224:1233	recurrent Clostridium difficile infections	1192:1233	recurrent Clostridium difficile infections	1192:1233	Fecal microbiota transplantation (FMT) highly effective for treating recurrent Clostridium difficile infections.
29748817	8	77	theme	health	1294:1299	arg1	aspects					1277:1283	virtually all aspects	1263:1283	virtually all aspects of human health	1263:1299	The gut microbiome affects virtually all aspects of human health, but the degree of scientific evidence, the models and technologies and the understanding of mechanisms of action vary considerably from one benefit area to the other.
29748817	9	78	theme	clinical	1475:1482	arg1	practice					1484:1491	a clinical practice	1473:1491	a clinical practice	1473:1491	For a clinical practice to be broadly accepted, the mode of action, the therapeutic window, and potential side effects need to thoroughly be investigated.
29748817	7	79	theme	Fecal	1123:1127	arg1	FMT					1157:1159	FMT	1157:1159	FMT	1157:1159	Fecal microbiota transplantation (FMT) highly effective for treating recurrent Clostridium difficile infections.
29748817	7	79	theme	Fecal	1123:1127	arg1	transplantation					1140:1154	Fecal microbiota transplantation	1123:1154	Fecal microbiota transplantation (FMT)	1123:1160	Fecal microbiota transplantation (FMT) highly effective for treating recurrent Clostridium difficile infections.
29748817	10	80	theme	gut	1729:1731	arg1	microbiome					1733:1742	human gut microbiome's	1723:1744	the human gut microbiome's effects on human health	1719:1768	This calls for further coordinated state-of-the art research to better understand and document the human gut microbiome's effects on human health.
29748817	3	81	theme	intrinsic	565:573	arg1	factors					575:581	intrinsic factors	565:581	intrinsic factors (such as stress)	565:598	The gut microbiota changes dramatically during pregnancy and intrinsic factors (such as stress), in addition to extrinsic factors (such as diet, and drugs) influence the composition and activity of the gut microbiome throughout life.
29748817	10	82	theme	further	1639:1645	arg1	research					1676:1683	further coordinated state-of-the art research	1639:1683	further coordinated state-of-the art research	1639:1683	This calls for further coordinated state-of-the art research to better understand and document the human gut microbiome's effects on human health.
29748817	2	83	theme	probiotics	331:340	arg1	interactions					303:314	interactions	303:314	interactions of prebiotics, probiotics, or vitamins with the gut microbiome in health and disease, the development of the microbiome in early-life and the role of the microbiome on the gut-brain axis	303:501	Experts from academia and industry examined interactions of prebiotics, probiotics, or vitamins with the gut microbiome in health and disease, the development of the microbiome in early-life and the role of the microbiome on the gut-brain axis.
29748817	3	84	theme	gut	706:708	arg1	microbiome					710:719	the gut microbiome	702:719	the gut microbiome	702:719	The gut microbiota changes dramatically during pregnancy and intrinsic factors (such as stress), in addition to extrinsic factors (such as diet, and drugs) influence the composition and activity of the gut microbiome throughout life.
29683418	2	0	theme	rod-	104:107	arg1	bacterium					125:133	A Gram-stain-positive, aerobic, motile, rod- or cocci-shaped bacterium	64:133	A Gram-stain-positive, aerobic, motile, rod- or cocci-shaped bacterium with flagella bacterium (THG-T63T)	64:168	A Gram-stain-positive, aerobic, motile, rod- or cocci-shaped bacterium with flagella bacterium (THG-T63T) was isolated from freshwater mud.
29683418	4	1	dep	Nocardioides	598:609	arg1	nitrophenolicus					611:625	nitrophenolicus	611:625	nitrophenolicus	611:625	Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T63T were identified as Nocardioides panacisoli KCTC 19470T (97.5 %), Nocardioides caeni KCTC 19600T (96.4 %), Nocardioides humi KCTC 19265T (96.3 %), Nocardioides kongjuensis KCTC 19054T (96.1 %) and Nocardioides nitrophenolicus KCTC 457BPT (96.1 %).
29683418	9	2	contain	contained	1025:1033	arg1	peptidoglycan					1011:1023	The cell-wall peptidoglycan	997:1023	The cell-wall peptidoglycan	997:1023	The cell-wall peptidoglycan contained ll-2,6-diaminopimelic acid.
29683418	9	2	contain	contained	1025:1033	arg2	acid					1057:1060	ll-2,6-diaminopimelic acid	1035:1060	ll-2,6-diaminopimelic acid	1035:1060	The cell-wall peptidoglycan contained ll-2,6-diaminopimelic acid.
29683418	4	3	theme	strain	386:391	arg1	THG-T63T					393:400	strain THG-T63T	386:400	strain THG-T63T	386:400	Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T63T were identified as Nocardioides panacisoli KCTC 19470T (97.5 %), Nocardioides caeni KCTC 19600T (96.4 %), Nocardioides humi KCTC 19265T (96.3 %), Nocardioides kongjuensis KCTC 19054T (96.1 %) and Nocardioides nitrophenolicus KCTC 457BPT (96.1 %).
29683418	11	4	theme	phenotypic	1324:1333	arg1	characteristics					1335:1349	phenotypic characteristics	1324:1349	phenotypic characteristics	1324:1349	Strain THG-T63T exhibited levels of DNA-DNA relatedness of 20-44 % to the type strains of phylogenetically related Nocardioides species and could be differentiated from these species based on differences in phenotypic characteristics.
29683418	3	5	theme	0-6 	283:286	arg1	%					287:287	%	287:287	%	287:287	Growth occurred at 10-40 °C (optimum, 28-35 °C), at pH 6-8 (optimum, 7) and at 0-6 % NaCl (optimum, 2 %).
29683418	2	6	theme	motile	96:101	arg1	bacterium					125:133	A Gram-stain-positive, aerobic, motile, rod- or cocci-shaped bacterium	64:133	A Gram-stain-positive, aerobic, motile, rod- or cocci-shaped bacterium with flagella bacterium (THG-T63T)	64:168	A Gram-stain-positive, aerobic, motile, rod- or cocci-shaped bacterium with flagella bacterium (THG-T63T) was isolated from freshwater mud.
29683418	3	7	dep	optimum	295:301	arg1	%					306:306	2 %	304:306	2 %	304:306	Growth occurred at 10-40 °C (optimum, 28-35 °C), at pH 6-8 (optimum, 7) and at 0-6 % NaCl (optimum, 2 %).
29683418	1	8	theme	freshwater	48:57	arg1	mud					59:61	freshwater mud	48:61	freshwater mud	48:61	nov., isolated from freshwater mud.
29683418	4	9	theme	caeni	480:484	arg1	neighbours					372:381	the nearest phylogenetic neighbours	347:381	the nearest phylogenetic neighbours of strain THG-T63T	347:400	Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T63T were identified as Nocardioides panacisoli KCTC 19470T (97.5 %), Nocardioides caeni KCTC 19600T (96.4 %), Nocardioides humi KCTC 19265T (96.3 %), Nocardioides kongjuensis KCTC 19054T (96.1 %) and Nocardioides nitrophenolicus KCTC 457BPT (96.1 %).
29683418	4	9	theme	caeni	480:484	arg1	19600T					491:496	Nocardioides caeni KCTC 19600T	467:496	Nocardioides caeni KCTC 19600T (96.4 %)	467:505	Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T63T were identified as Nocardioides panacisoli KCTC 19470T (97.5 %), Nocardioides caeni KCTC 19600T (96.4 %), Nocardioides humi KCTC 19265T (96.3 %), Nocardioides kongjuensis KCTC 19054T (96.1 %) and Nocardioides nitrophenolicus KCTC 457BPT (96.1 %).
29683418	4	9	theme	caeni	480:484	arg1	%					504:504	96.4 %	499:504	96.4 %	499:504	Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T63T were identified as Nocardioides panacisoli KCTC 19470T (97.5 %), Nocardioides caeni KCTC 19600T (96.4 %), Nocardioides humi KCTC 19265T (96.3 %), Nocardioides kongjuensis KCTC 19054T (96.1 %) and Nocardioides nitrophenolicus KCTC 457BPT (96.1 %).
29683418	3	10	dep	pH	256:257	arg1	optimum					264:270	optimum	264:270	optimum	264:270	Growth occurred at 10-40 °C (optimum, 28-35 °C), at pH 6-8 (optimum, 7) and at 0-6 % NaCl (optimum, 2 %).
29683418	3	10	dep	pH	256:257	arg1	6-8					259:261	6-8	259:261	6-8	259:261	Growth occurred at 10-40 °C (optimum, 28-35 °C), at pH 6-8 (optimum, 7) and at 0-6 % NaCl (optimum, 2 %).
29683418	4	11	theme	phylogenetic	359:370	arg1	19470T					450:455	Nocardioides panacisoli KCTC 19470T	421:455	Nocardioides panacisoli KCTC 19470T (97.5 %)	421:464	Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T63T were identified as Nocardioides panacisoli KCTC 19470T (97.5 %), Nocardioides caeni KCTC 19600T (96.4 %), Nocardioides humi KCTC 19265T (96.3 %), Nocardioides kongjuensis KCTC 19054T (96.1 %) and Nocardioides nitrophenolicus KCTC 457BPT (96.1 %).
29683418	4	11	theme	phylogenetic	359:370	arg1	19054T					578:583	Nocardioides kongjuensis KCTC 19054T	548:583	Nocardioides kongjuensis KCTC 19054T (96.1 %)	548:592	Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T63T were identified as Nocardioides panacisoli KCTC 19470T (97.5 %), Nocardioides caeni KCTC 19600T (96.4 %), Nocardioides humi KCTC 19265T (96.3 %), Nocardioides kongjuensis KCTC 19054T (96.1 %) and Nocardioides nitrophenolicus KCTC 457BPT (96.1 %).
29683418	4	11	theme	phylogenetic	359:370	arg1	neighbours					372:381	the nearest phylogenetic neighbours	347:381	the nearest phylogenetic neighbours of strain THG-T63T	347:400	Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T63T were identified as Nocardioides panacisoli KCTC 19470T (97.5 %), Nocardioides caeni KCTC 19600T (96.4 %), Nocardioides humi KCTC 19265T (96.3 %), Nocardioides kongjuensis KCTC 19054T (96.1 %) and Nocardioides nitrophenolicus KCTC 457BPT (96.1 %).
29683418	4	11	theme	phylogenetic	359:370	arg1	19600T					491:496	Nocardioides caeni KCTC 19600T	467:496	Nocardioides caeni KCTC 19600T (96.4 %)	467:505	Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T63T were identified as Nocardioides panacisoli KCTC 19470T (97.5 %), Nocardioides caeni KCTC 19600T (96.4 %), Nocardioides humi KCTC 19265T (96.3 %), Nocardioides kongjuensis KCTC 19054T (96.1 %) and Nocardioides nitrophenolicus KCTC 457BPT (96.1 %).
29683418	4	11	theme	phylogenetic	359:370	arg1	457BPT					632:637	Nocardioides nitrophenolicus KCTC 457BPT	598:637	Nocardioides nitrophenolicus KCTC 457BPT (96.1 %)	598:646	Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T63T were identified as Nocardioides panacisoli KCTC 19470T (97.5 %), Nocardioides caeni KCTC 19600T (96.4 %), Nocardioides humi KCTC 19265T (96.3 %), Nocardioides kongjuensis KCTC 19054T (96.1 %) and Nocardioides nitrophenolicus KCTC 457BPT (96.1 %).
29683418	4	11	theme	phylogenetic	359:370	arg1	19265T					531:536	Nocardioides humi KCTC 19265T	508:536	Nocardioides humi KCTC 19265T (96.3 %)	508:545	Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T63T were identified as Nocardioides panacisoli KCTC 19470T (97.5 %), Nocardioides caeni KCTC 19600T (96.4 %), Nocardioides humi KCTC 19265T (96.3 %), Nocardioides kongjuensis KCTC 19054T (96.1 %) and Nocardioides nitrophenolicus KCTC 457BPT (96.1 %).
29683418	10	12	theme	DNA	1067:1069	arg1	%					1114:1114	74.6 mol%	1106:1114	74.6 mol%	1106:1114	The DNA G+C content of strain THG-T63T was 74.6 mol%.
29683418	10	12	theme	DNA	1067:1069	arg1	G+C content					1071:1081	The DNA G+C content	1063:1081	The DNA G+C content of strain THG-T63T	1063:1100	The DNA G+C content of strain THG-T63T was 74.6 mol%.
29683418	4	13	theme	Nocardioides	467:478	arg1	neighbours					372:381	the nearest phylogenetic neighbours	347:381	the nearest phylogenetic neighbours of strain THG-T63T	347:400	Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T63T were identified as Nocardioides panacisoli KCTC 19470T (97.5 %), Nocardioides caeni KCTC 19600T (96.4 %), Nocardioides humi KCTC 19265T (96.3 %), Nocardioides kongjuensis KCTC 19054T (96.1 %) and Nocardioides nitrophenolicus KCTC 457BPT (96.1 %).
29683418	4	13	theme	Nocardioides	467:478	arg1	19600T					491:496	Nocardioides caeni KCTC 19600T	467:496	Nocardioides caeni KCTC 19600T (96.4 %)	467:505	Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T63T were identified as Nocardioides panacisoli KCTC 19470T (97.5 %), Nocardioides caeni KCTC 19600T (96.4 %), Nocardioides humi KCTC 19265T (96.3 %), Nocardioides kongjuensis KCTC 19054T (96.1 %) and Nocardioides nitrophenolicus KCTC 457BPT (96.1 %).
29683418	4	13	theme	Nocardioides	467:478	arg1	%					504:504	96.4 %	499:504	96.4 %	499:504	Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T63T were identified as Nocardioides panacisoli KCTC 19470T (97.5 %), Nocardioides caeni KCTC 19600T (96.4 %), Nocardioides humi KCTC 19265T (96.3 %), Nocardioides kongjuensis KCTC 19054T (96.1 %) and Nocardioides nitrophenolicus KCTC 457BPT (96.1 %).
29683418	4	14	theme	nearest	351:357	arg1	19470T					450:455	Nocardioides panacisoli KCTC 19470T	421:455	Nocardioides panacisoli KCTC 19470T (97.5 %)	421:464	Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T63T were identified as Nocardioides panacisoli KCTC 19470T (97.5 %), Nocardioides caeni KCTC 19600T (96.4 %), Nocardioides humi KCTC 19265T (96.3 %), Nocardioides kongjuensis KCTC 19054T (96.1 %) and Nocardioides nitrophenolicus KCTC 457BPT (96.1 %).
29683418	4	14	theme	nearest	351:357	arg1	19054T					578:583	Nocardioides kongjuensis KCTC 19054T	548:583	Nocardioides kongjuensis KCTC 19054T (96.1 %)	548:592	Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T63T were identified as Nocardioides panacisoli KCTC 19470T (97.5 %), Nocardioides caeni KCTC 19600T (96.4 %), Nocardioides humi KCTC 19265T (96.3 %), Nocardioides kongjuensis KCTC 19054T (96.1 %) and Nocardioides nitrophenolicus KCTC 457BPT (96.1 %).
29683418	4	14	theme	nearest	351:357	arg1	neighbours					372:381	the nearest phylogenetic neighbours	347:381	the nearest phylogenetic neighbours of strain THG-T63T	347:400	Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T63T were identified as Nocardioides panacisoli KCTC 19470T (97.5 %), Nocardioides caeni KCTC 19600T (96.4 %), Nocardioides humi KCTC 19265T (96.3 %), Nocardioides kongjuensis KCTC 19054T (96.1 %) and Nocardioides nitrophenolicus KCTC 457BPT (96.1 %).
29683418	4	14	theme	nearest	351:357	arg1	19600T					491:496	Nocardioides caeni KCTC 19600T	467:496	Nocardioides caeni KCTC 19600T (96.4 %)	467:505	Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T63T were identified as Nocardioides panacisoli KCTC 19470T (97.5 %), Nocardioides caeni KCTC 19600T (96.4 %), Nocardioides humi KCTC 19265T (96.3 %), Nocardioides kongjuensis KCTC 19054T (96.1 %) and Nocardioides nitrophenolicus KCTC 457BPT (96.1 %).
29683418	4	14	theme	nearest	351:357	arg1	457BPT					632:637	Nocardioides nitrophenolicus KCTC 457BPT	598:637	Nocardioides nitrophenolicus KCTC 457BPT (96.1 %)	598:646	Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T63T were identified as Nocardioides panacisoli KCTC 19470T (97.5 %), Nocardioides caeni KCTC 19600T (96.4 %), Nocardioides humi KCTC 19265T (96.3 %), Nocardioides kongjuensis KCTC 19054T (96.1 %) and Nocardioides nitrophenolicus KCTC 457BPT (96.1 %).
29683418	4	14	theme	nearest	351:357	arg1	19265T					531:536	Nocardioides humi KCTC 19265T	508:536	Nocardioides humi KCTC 19265T (96.3 %)	508:545	Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T63T were identified as Nocardioides panacisoli KCTC 19470T (97.5 %), Nocardioides caeni KCTC 19600T (96.4 %), Nocardioides humi KCTC 19265T (96.3 %), Nocardioides kongjuensis KCTC 19054T (96.1 %) and Nocardioides nitrophenolicus KCTC 457BPT (96.1 %).
29683418	14	15	theme	type	1532:1535	arg1	THG-T63T					1547:1554	THG-T63T	1547:1554	THG-T63T(=KACC 19192T=CGMCC 4.7388T)	1547:1582	The type strain is THG-T63T(=KACC 19192T=CGMCC 4.7388T).
29683418	14	15	theme	type	1532:1535	arg1	strain					1537:1542	The type strain	1528:1542	The type strain	1528:1542	The type strain is THG-T63T(=KACC 19192T=CGMCC 4.7388T).
29683418	4	16	theme	KCTC	627:630	arg1	neighbours					372:381	the nearest phylogenetic neighbours	347:381	the nearest phylogenetic neighbours of strain THG-T63T	347:400	Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T63T were identified as Nocardioides panacisoli KCTC 19470T (97.5 %), Nocardioides caeni KCTC 19600T (96.4 %), Nocardioides humi KCTC 19265T (96.3 %), Nocardioides kongjuensis KCTC 19054T (96.1 %) and Nocardioides nitrophenolicus KCTC 457BPT (96.1 %).
29683418	4	16	theme	KCTC	627:630	arg1	457BPT					632:637	Nocardioides nitrophenolicus KCTC 457BPT	598:637	Nocardioides nitrophenolicus KCTC 457BPT (96.1 %)	598:646	Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T63T were identified as Nocardioides panacisoli KCTC 19470T (97.5 %), Nocardioides caeni KCTC 19600T (96.4 %), Nocardioides humi KCTC 19265T (96.3 %), Nocardioides kongjuensis KCTC 19054T (96.1 %) and Nocardioides nitrophenolicus KCTC 457BPT (96.1 %).
29683418	4	16	theme	KCTC	627:630	arg1	%					645:645	96.1 %	640:645	96.1 %	640:645	Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T63T were identified as Nocardioides panacisoli KCTC 19470T (97.5 %), Nocardioides caeni KCTC 19600T (96.4 %), Nocardioides humi KCTC 19265T (96.3 %), Nocardioides kongjuensis KCTC 19054T (96.1 %) and Nocardioides nitrophenolicus KCTC 457BPT (96.1 %).
29683418	4	17	theme	KCTC	526:529	arg1	neighbours					372:381	the nearest phylogenetic neighbours	347:381	the nearest phylogenetic neighbours of strain THG-T63T	347:400	Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T63T were identified as Nocardioides panacisoli KCTC 19470T (97.5 %), Nocardioides caeni KCTC 19600T (96.4 %), Nocardioides humi KCTC 19265T (96.3 %), Nocardioides kongjuensis KCTC 19054T (96.1 %) and Nocardioides nitrophenolicus KCTC 457BPT (96.1 %).
29683418	4	17	theme	KCTC	526:529	arg1	19265T					531:536	Nocardioides humi KCTC 19265T	508:536	Nocardioides humi KCTC 19265T (96.3 %)	508:545	Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T63T were identified as Nocardioides panacisoli KCTC 19470T (97.5 %), Nocardioides caeni KCTC 19600T (96.4 %), Nocardioides humi KCTC 19265T (96.3 %), Nocardioides kongjuensis KCTC 19054T (96.1 %) and Nocardioides nitrophenolicus KCTC 457BPT (96.1 %).
29683418	4	17	theme	KCTC	526:529	arg1	%					544:544	96.3 %	539:544	96.3 %	539:544	Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T63T were identified as Nocardioides panacisoli KCTC 19470T (97.5 %), Nocardioides caeni KCTC 19600T (96.4 %), Nocardioides humi KCTC 19265T (96.3 %), Nocardioides kongjuensis KCTC 19054T (96.1 %) and Nocardioides nitrophenolicus KCTC 457BPT (96.1 %).
29683418	11	18	theme	%	1182:1182	arg1	relatedness					1161:1171	DNA-DNA relatedness	1153:1171	DNA-DNA relatedness of 20-44 % to the type strains of phylogenetically related Nocardioides species	1153:1251	Strain THG-T63T exhibited levels of DNA-DNA relatedness of 20-44 % to the type strains of phylogenetically related Nocardioides species and could be differentiated from these species based on differences in phenotypic characteristics.
29683418	9	19	theme	ll-2,6-diaminopimelic	1035:1055	arg1	acid					1057:1060	ll-2,6-diaminopimelic acid	1035:1060	ll-2,6-diaminopimelic acid	1035:1060	The cell-wall peptidoglycan contained ll-2,6-diaminopimelic acid.
29683418	7	20	theme	quinone	848:854	arg1	system					856:861	The quinone system	844:861	The quinone system	844:861	The quinone system was composed of MK-8 (H4).
29683418	11	21	theme	type	1191:1194	arg1	strains					1196:1202	the type strains	1187:1202	the type strains of phylogenetically related Nocardioides species	1187:1251	Strain THG-T63T exhibited levels of DNA-DNA relatedness of 20-44 % to the type strains of phylogenetically related Nocardioides species and could be differentiated from these species based on differences in phenotypic characteristics.
29683418	4	22	theme	humi	521:524	arg1	neighbours					372:381	the nearest phylogenetic neighbours	347:381	the nearest phylogenetic neighbours of strain THG-T63T	347:400	Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T63T were identified as Nocardioides panacisoli KCTC 19470T (97.5 %), Nocardioides caeni KCTC 19600T (96.4 %), Nocardioides humi KCTC 19265T (96.3 %), Nocardioides kongjuensis KCTC 19054T (96.1 %) and Nocardioides nitrophenolicus KCTC 457BPT (96.1 %).
29683418	4	22	theme	humi	521:524	arg1	19265T					531:536	Nocardioides humi KCTC 19265T	508:536	Nocardioides humi KCTC 19265T (96.3 %)	508:545	Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T63T were identified as Nocardioides panacisoli KCTC 19470T (97.5 %), Nocardioides caeni KCTC 19600T (96.4 %), Nocardioides humi KCTC 19265T (96.3 %), Nocardioides kongjuensis KCTC 19054T (96.1 %) and Nocardioides nitrophenolicus KCTC 457BPT (96.1 %).
29683418	4	22	theme	humi	521:524	arg1	%					544:544	96.3 %	539:544	96.3 %	539:544	Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T63T were identified as Nocardioides panacisoli KCTC 19470T (97.5 %), Nocardioides caeni KCTC 19600T (96.4 %), Nocardioides humi KCTC 19265T (96.3 %), Nocardioides kongjuensis KCTC 19054T (96.1 %) and Nocardioides nitrophenolicus KCTC 457BPT (96.1 %).
29683418	0	23	theme	pelophilus	13:22	arg1	sp					24:25	Nocardioides pelophilus sp	0:25	Nocardioides pelophilus sp.	0:26	Nocardioides pelophilus sp.
29683418	2	24	theme	cocci-shaped	112:123	arg1	bacterium					125:133	A Gram-stain-positive, aerobic, motile, rod- or cocci-shaped bacterium	64:133	A Gram-stain-positive, aerobic, motile, rod- or cocci-shaped bacterium with flagella bacterium (THG-T63T)	64:168	A Gram-stain-positive, aerobic, motile, rod- or cocci-shaped bacterium with flagella bacterium (THG-T63T) was isolated from freshwater mud.
29683418	8	25	theme	fatty	900:904	arg1	C16 					917:920	C16 	917:920	C16 	917:920	The major fatty acids were C16 : 0, C17 : 1ω6c, C17 : 1ω8c, C18 : 0 10-methyl, C18 : 1ω9c and iso-C16 : 0.
29683418	8	25	theme	fatty	900:904	arg1	acids					906:910	The major fatty acids	890:910	The major fatty acids	890:910	The major fatty acids were C16 : 0, C17 : 1ω6c, C17 : 1ω8c, C18 : 0 10-methyl, C18 : 1ω9c and iso-C16 : 0.
29683418	12	26	theme	genus	1443:1447	arg1	Nocardioides					1449:1460	the genus Nocardioides	1439:1460	the genus Nocardioides	1439:1460	On the basis of the data presented here, strain THG-T63T represents a novel species of the genus Nocardioides, for which the name Nocardioides pelophilus sp.
29683418	5	27	theme	other	706:710	arg1	species					712:718	other species	706:718	other species	706:718	16S rRNA sequence similarities among strain THG-T63T and other species were lower than 96.0 %.
29683418	0	28	theme	Nocardioides	0:11	arg1	sp					24:25	Nocardioides pelophilus sp	0:25	Nocardioides pelophilus sp.	0:26	Nocardioides pelophilus sp.
29683418	6	29	theme	unidentified	817:828	arg1	phospholipid					830:841	one unidentified phospholipid	813:841	one unidentified phospholipid	813:841	The polar lipids were phosphatidylglycerol, phosphatidylinositol and one unidentified phospholipid.
29683418	8	30	theme	major	894:898	arg1	C16 					917:920	C16 	917:920	C16 	917:920	The major fatty acids were C16 : 0, C17 : 1ω6c, C17 : 1ω8c, C18 : 0 10-methyl, C18 : 1ω9c and iso-C16 : 0.
29683418	8	30	theme	major	894:898	arg1	acids					906:910	The major fatty acids	890:910	The major fatty acids	890:910	The major fatty acids were C16 : 0, C17 : 1ω6c, C17 : 1ω8c, C18 : 0 10-methyl, C18 : 1ω9c and iso-C16 : 0.
29683418	12	31	dep	Nocardioides	1482:1493	arg1	pelophilus					1495:1504	pelophilus	1495:1504	pelophilus	1495:1504	On the basis of the data presented here, strain THG-T63T represents a novel species of the genus Nocardioides, for which the name Nocardioides pelophilus sp.
29683418	9	32	theme	cell-wall	1001:1009	arg1	peptidoglycan					1011:1023	The cell-wall peptidoglycan	997:1023	The cell-wall peptidoglycan	997:1023	The cell-wall peptidoglycan contained ll-2,6-diaminopimelic acid.
29683418	10	33	theme	THG-T63T	1093:1100	arg1	%					1114:1114	74.6 mol%	1106:1114	74.6 mol%	1106:1114	The DNA G+C content of strain THG-T63T was 74.6 mol%.
29683418	10	33	theme	THG-T63T	1093:1100	arg1	G+C content					1071:1081	The DNA G+C content	1063:1081	The DNA G+C content of strain THG-T63T	1063:1100	The DNA G+C content of strain THG-T63T was 74.6 mol%.
29683418	2	34	attach	isolated	174:181	arg2	bacterium					125:133	A Gram-stain-positive, aerobic, motile, rod- or cocci-shaped bacterium	64:133	A Gram-stain-positive, aerobic, motile, rod- or cocci-shaped bacterium with flagella bacterium (THG-T63T)	64:168	A Gram-stain-positive, aerobic, motile, rod- or cocci-shaped bacterium with flagella bacterium (THG-T63T) was isolated from freshwater mud.
29683418	2	34	attach	isolated	174:181	arg1	mud					199:201	freshwater mud	188:201	freshwater mud	188:201	A Gram-stain-positive, aerobic, motile, rod- or cocci-shaped bacterium with flagella bacterium (THG-T63T) was isolated from freshwater mud.
29683418	5	35	theme	16S	649:651	arg1	rRNA					653:656	16S rRNA	649:656	16S rRNA sequence similarities among strain THG-T63T and other species	649:718	16S rRNA sequence similarities among strain THG-T63T and other species were lower than 96.0 %.
29683418	3	36	dep	optimum	264:270	arg1	7					273:273	7	273:273	7	273:273	Growth occurred at 10-40 °C (optimum, 28-35 °C), at pH 6-8 (optimum, 7) and at 0-6 % NaCl (optimum, 2 %).
29683418	10	37	theme	strain	1086:1091	arg1	THG-T63T					1093:1100	strain THG-T63T	1086:1100	strain THG-T63T	1086:1100	The DNA G+C content of strain THG-T63T was 74.6 mol%.
29683418	2	38	with	bacterium	125:133	arg1	flagella					140:147	flagella bacterium (THG-T63T)	140:168	flagella bacterium (THG-T63T)	140:168	A Gram-stain-positive, aerobic, motile, rod- or cocci-shaped bacterium with flagella bacterium (THG-T63T) was isolated from freshwater mud.
29683418	5	39	theme	rRNA	653:656	arg1	similarities					667:678	16S rRNA sequence similarities	649:678	16S rRNA sequence similarities among strain THG-T63T and other species	649:718	16S rRNA sequence similarities among strain THG-T63T and other species were lower than 96.0 %.
29683418	2	40	dep	flagella	140:147	arg1	THG-T63T					160:167	THG-T63T	160:167	THG-T63T	160:167	A Gram-stain-positive, aerobic, motile, rod- or cocci-shaped bacterium with flagella bacterium (THG-T63T) was isolated from freshwater mud.
29683418	2	40	dep	flagella	140:147	arg1	bacterium					149:157	bacterium	149:157	flagella bacterium (THG-T63T)	140:168	A Gram-stain-positive, aerobic, motile, rod- or cocci-shaped bacterium with flagella bacterium (THG-T63T) was isolated from freshwater mud.
29683418	4	41	dep	Nocardioides	548:559	arg1	kongjuensis					561:571	kongjuensis	561:571	kongjuensis	561:571	Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T63T were identified as Nocardioides panacisoli KCTC 19470T (97.5 %), Nocardioides caeni KCTC 19600T (96.4 %), Nocardioides humi KCTC 19265T (96.3 %), Nocardioides kongjuensis KCTC 19054T (96.1 %) and Nocardioides nitrophenolicus KCTC 457BPT (96.1 %).
29683418	11	42	theme	related	1224:1230	arg1	species					1245:1251	phylogenetically related Nocardioides species	1207:1251	phylogenetically related Nocardioides species	1207:1251	Strain THG-T63T exhibited levels of DNA-DNA relatedness of 20-44 % to the type strains of phylogenetically related Nocardioides species and could be differentiated from these species based on differences in phenotypic characteristics.
29683418	11	43	theme	species	1245:1251	arg1	strains					1196:1202	the type strains	1187:1202	the type strains of phylogenetically related Nocardioides species	1187:1251	Strain THG-T63T exhibited levels of DNA-DNA relatedness of 20-44 % to the type strains of phylogenetically related Nocardioides species and could be differentiated from these species based on differences in phenotypic characteristics.
29683418	12	44	theme	strain	1393:1398	arg1	THG-T63T					1400:1407	strain THG-T63T	1393:1407	strain THG-T63T	1393:1407	On the basis of the data presented here, strain THG-T63T represents a novel species of the genus Nocardioides, for which the name Nocardioides pelophilus sp.
29683418	4	45	theme	sequence	328:335	arg1	analysis					337:344	16S rRNA sequence analysis	319:344	16S rRNA sequence analysis	319:344	Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T63T were identified as Nocardioides panacisoli KCTC 19470T (97.5 %), Nocardioides caeni KCTC 19600T (96.4 %), Nocardioides humi KCTC 19265T (96.3 %), Nocardioides kongjuensis KCTC 19054T (96.1 %) and Nocardioides nitrophenolicus KCTC 457BPT (96.1 %).
29683418	11	46	theme	Nocardioides	1232:1243	arg1	species					1245:1251	phylogenetically related Nocardioides species	1207:1251	phylogenetically related Nocardioides species	1207:1251	Strain THG-T63T exhibited levels of DNA-DNA relatedness of 20-44 % to the type strains of phylogenetically related Nocardioides species and could be differentiated from these species based on differences in phenotypic characteristics.
29683418	4	47	theme	Nocardioides	508:519	arg1	neighbours					372:381	the nearest phylogenetic neighbours	347:381	the nearest phylogenetic neighbours of strain THG-T63T	347:400	Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T63T were identified as Nocardioides panacisoli KCTC 19470T (97.5 %), Nocardioides caeni KCTC 19600T (96.4 %), Nocardioides humi KCTC 19265T (96.3 %), Nocardioides kongjuensis KCTC 19054T (96.1 %) and Nocardioides nitrophenolicus KCTC 457BPT (96.1 %).
29683418	4	47	theme	Nocardioides	508:519	arg1	19265T					531:536	Nocardioides humi KCTC 19265T	508:536	Nocardioides humi KCTC 19265T (96.3 %)	508:545	Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T63T were identified as Nocardioides panacisoli KCTC 19470T (97.5 %), Nocardioides caeni KCTC 19600T (96.4 %), Nocardioides humi KCTC 19265T (96.3 %), Nocardioides kongjuensis KCTC 19054T (96.1 %) and Nocardioides nitrophenolicus KCTC 457BPT (96.1 %).
29683418	4	47	theme	Nocardioides	508:519	arg1	%					544:544	96.3 %	539:544	96.3 %	539:544	Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T63T were identified as Nocardioides panacisoli KCTC 19470T (97.5 %), Nocardioides caeni KCTC 19600T (96.4 %), Nocardioides humi KCTC 19265T (96.3 %), Nocardioides kongjuensis KCTC 19054T (96.1 %) and Nocardioides nitrophenolicus KCTC 457BPT (96.1 %).
29683418	8	48	dep	C16 	917:920	arg1	C18 					969:972	C18 	969:972	C18 	969:972	The major fatty acids were C16 : 0, C17 : 1ω6c, C17 : 1ω8c, C18 : 0 10-methyl, C18 : 1ω9c and iso-C16 : 0.
29683418	8	48	dep	C16 	917:920	arg1	 0					993:994	 0	993:994	 0	993:994	The major fatty acids were C16 : 0, C17 : 1ω6c, C17 : 1ω8c, C18 : 0 10-methyl, C18 : 1ω9c and iso-C16 : 0.
29683418	8	48	dep	C16 	917:920	arg1	C18 					950:953	C18 	950:953	C18 	950:953	The major fatty acids were C16 : 0, C17 : 1ω6c, C17 : 1ω8c, C18 : 0 10-methyl, C18 : 1ω9c and iso-C16 : 0.
29683418	8	48	dep	C16 	917:920	arg1	 1ω9c					974:978	 1ω9c	974:978	 1ω9c	974:978	The major fatty acids were C16 : 0, C17 : 1ω6c, C17 : 1ω8c, C18 : 0 10-methyl, C18 : 1ω9c and iso-C16 : 0.
29683418	8	48	dep	C16 	917:920	arg1	iso-C16 					984:991	iso-C16 	984:991	iso-C16 	984:991	The major fatty acids were C16 : 0, C17 : 1ω6c, C17 : 1ω8c, C18 : 0 10-methyl, C18 : 1ω9c and iso-C16 : 0.
29683418	8	48	dep	C16 	917:920	arg1	C17 					938:941	C17 	938:941	C17 	938:941	The major fatty acids were C16 : 0, C17 : 1ω6c, C17 : 1ω8c, C18 : 0 10-methyl, C18 : 1ω9c and iso-C16 : 0.
29683418	4	49	theme	Nocardioides	421:432	arg1	neighbours					372:381	the nearest phylogenetic neighbours	347:381	the nearest phylogenetic neighbours of strain THG-T63T	347:400	Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T63T were identified as Nocardioides panacisoli KCTC 19470T (97.5 %), Nocardioides caeni KCTC 19600T (96.4 %), Nocardioides humi KCTC 19265T (96.3 %), Nocardioides kongjuensis KCTC 19054T (96.1 %) and Nocardioides nitrophenolicus KCTC 457BPT (96.1 %).
29683418	4	49	theme	Nocardioides	421:432	arg1	19470T					450:455	Nocardioides panacisoli KCTC 19470T	421:455	Nocardioides panacisoli KCTC 19470T (97.5 %)	421:464	Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T63T were identified as Nocardioides panacisoli KCTC 19470T (97.5 %), Nocardioides caeni KCTC 19600T (96.4 %), Nocardioides humi KCTC 19265T (96.3 %), Nocardioides kongjuensis KCTC 19054T (96.1 %) and Nocardioides nitrophenolicus KCTC 457BPT (96.1 %).
29683418	4	49	theme	Nocardioides	421:432	arg1	%					463:463	97.5 %	458:463	97.5 %	458:463	Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T63T were identified as Nocardioides panacisoli KCTC 19470T (97.5 %), Nocardioides caeni KCTC 19600T (96.4 %), Nocardioides humi KCTC 19265T (96.3 %), Nocardioides kongjuensis KCTC 19054T (96.1 %) and Nocardioides nitrophenolicus KCTC 457BPT (96.1 %).
29683418	5	50	theme	strain	686:691	arg1	THG-T63T					693:700	strain THG-T63T	686:700	strain THG-T63T	686:700	16S rRNA sequence similarities among strain THG-T63T and other species were lower than 96.0 %.
29683418	4	51	theme	panacisoli	434:443	arg1	neighbours					372:381	the nearest phylogenetic neighbours	347:381	the nearest phylogenetic neighbours of strain THG-T63T	347:400	Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T63T were identified as Nocardioides panacisoli KCTC 19470T (97.5 %), Nocardioides caeni KCTC 19600T (96.4 %), Nocardioides humi KCTC 19265T (96.3 %), Nocardioides kongjuensis KCTC 19054T (96.1 %) and Nocardioides nitrophenolicus KCTC 457BPT (96.1 %).
29683418	4	51	theme	panacisoli	434:443	arg1	19470T					450:455	Nocardioides panacisoli KCTC 19470T	421:455	Nocardioides panacisoli KCTC 19470T (97.5 %)	421:464	Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T63T were identified as Nocardioides panacisoli KCTC 19470T (97.5 %), Nocardioides caeni KCTC 19600T (96.4 %), Nocardioides humi KCTC 19265T (96.3 %), Nocardioides kongjuensis KCTC 19054T (96.1 %) and Nocardioides nitrophenolicus KCTC 457BPT (96.1 %).
29683418	4	51	theme	panacisoli	434:443	arg1	%					463:463	97.5 %	458:463	97.5 %	458:463	Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T63T were identified as Nocardioides panacisoli KCTC 19470T (97.5 %), Nocardioides caeni KCTC 19600T (96.4 %), Nocardioides humi KCTC 19265T (96.3 %), Nocardioides kongjuensis KCTC 19054T (96.1 %) and Nocardioides nitrophenolicus KCTC 457BPT (96.1 %).
29683418	11	52	theme	Strain	1117:1122	arg1	THG-T63T					1124:1131	Strain THG-T63T	1117:1131	Strain THG-T63T	1117:1131	Strain THG-T63T exhibited levels of DNA-DNA relatedness of 20-44 % to the type strains of phylogenetically related Nocardioides species and could be differentiated from these species based on differences in phenotypic characteristics.
29683418	2	53	theme	aerobic	87:93	arg1	bacterium					125:133	A Gram-stain-positive, aerobic, motile, rod- or cocci-shaped bacterium	64:133	A Gram-stain-positive, aerobic, motile, rod- or cocci-shaped bacterium with flagella bacterium (THG-T63T)	64:168	A Gram-stain-positive, aerobic, motile, rod- or cocci-shaped bacterium with flagella bacterium (THG-T63T) was isolated from freshwater mud.
29683418	3	54	dep	optimum	233:239	arg1	28-35 °C					242:249	28-35 °C	242:249	28-35 °C	242:249	Growth occurred at 10-40 °C (optimum, 28-35 °C), at pH 6-8 (optimum, 7) and at 0-6 % NaCl (optimum, 2 %).
29683418	4	55	theme	KCTC	445:448	arg1	neighbours					372:381	the nearest phylogenetic neighbours	347:381	the nearest phylogenetic neighbours of strain THG-T63T	347:400	Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T63T were identified as Nocardioides panacisoli KCTC 19470T (97.5 %), Nocardioides caeni KCTC 19600T (96.4 %), Nocardioides humi KCTC 19265T (96.3 %), Nocardioides kongjuensis KCTC 19054T (96.1 %) and Nocardioides nitrophenolicus KCTC 457BPT (96.1 %).
29683418	4	55	theme	KCTC	445:448	arg1	19470T					450:455	Nocardioides panacisoli KCTC 19470T	421:455	Nocardioides panacisoli KCTC 19470T (97.5 %)	421:464	Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T63T were identified as Nocardioides panacisoli KCTC 19470T (97.5 %), Nocardioides caeni KCTC 19600T (96.4 %), Nocardioides humi KCTC 19265T (96.3 %), Nocardioides kongjuensis KCTC 19054T (96.1 %) and Nocardioides nitrophenolicus KCTC 457BPT (96.1 %).
29683418	4	55	theme	KCTC	445:448	arg1	%					463:463	97.5 %	458:463	97.5 %	458:463	Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T63T were identified as Nocardioides panacisoli KCTC 19470T (97.5 %), Nocardioides caeni KCTC 19600T (96.4 %), Nocardioides humi KCTC 19265T (96.3 %), Nocardioides kongjuensis KCTC 19054T (96.1 %) and Nocardioides nitrophenolicus KCTC 457BPT (96.1 %).
29683418	6	56	theme	polar	748:752	arg1	phosphatidylglycerol					766:785	phosphatidylglycerol	766:785	phosphatidylglycerol	766:785	The polar lipids were phosphatidylglycerol, phosphatidylinositol and one unidentified phospholipid.
29683418	6	56	theme	polar	748:752	arg1	lipids					754:759	The polar lipids	744:759	The polar lipids	744:759	The polar lipids were phosphatidylglycerol, phosphatidylinositol and one unidentified phospholipid.
29683418	2	57	theme	freshwater	188:197	arg1	mud					199:201	freshwater mud	188:201	freshwater mud	188:201	A Gram-stain-positive, aerobic, motile, rod- or cocci-shaped bacterium with flagella bacterium (THG-T63T) was isolated from freshwater mud.
29683418	10	58	theme	74.6 mol	1106:1113	arg1	%					1114:1114	74.6 mol%	1106:1114	74.6 mol%	1106:1114	The DNA G+C content of strain THG-T63T was 74.6 mol%.
29683418	10	58	theme	74.6 mol	1106:1113	arg1	G+C content					1071:1081	The DNA G+C content	1063:1081	The DNA G+C content of strain THG-T63T	1063:1100	The DNA G+C content of strain THG-T63T was 74.6 mol%.
29683418	4	59	theme	Nocardioides	548:559	arg1	%					591:591	96.1 %	586:591	96.1 %	586:591	Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T63T were identified as Nocardioides panacisoli KCTC 19470T (97.5 %), Nocardioides caeni KCTC 19600T (96.4 %), Nocardioides humi KCTC 19265T (96.3 %), Nocardioides kongjuensis KCTC 19054T (96.1 %) and Nocardioides nitrophenolicus KCTC 457BPT (96.1 %).
29683418	4	59	theme	Nocardioides	548:559	arg1	neighbours					372:381	the nearest phylogenetic neighbours	347:381	the nearest phylogenetic neighbours of strain THG-T63T	347:400	Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T63T were identified as Nocardioides panacisoli KCTC 19470T (97.5 %), Nocardioides caeni KCTC 19600T (96.4 %), Nocardioides humi KCTC 19265T (96.3 %), Nocardioides kongjuensis KCTC 19054T (96.1 %) and Nocardioides nitrophenolicus KCTC 457BPT (96.1 %).
29683418	4	59	theme	Nocardioides	548:559	arg1	19054T					578:583	Nocardioides kongjuensis KCTC 19054T	548:583	Nocardioides kongjuensis KCTC 19054T (96.1 %)	548:592	Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T63T were identified as Nocardioides panacisoli KCTC 19470T (97.5 %), Nocardioides caeni KCTC 19600T (96.4 %), Nocardioides humi KCTC 19265T (96.3 %), Nocardioides kongjuensis KCTC 19054T (96.1 %) and Nocardioides nitrophenolicus KCTC 457BPT (96.1 %).
29683418	12	60	theme	Nocardioides	1482:1493	arg1	sp					1506:1507	the name Nocardioides pelophilus sp	1473:1507	the name Nocardioides pelophilus sp	1473:1507	On the basis of the data presented here, strain THG-T63T represents a novel species of the genus Nocardioides, for which the name Nocardioides pelophilus sp.
29683418	2	61	theme	Gram-stain-positive	66:84	arg1	bacterium					125:133	A Gram-stain-positive, aerobic, motile, rod- or cocci-shaped bacterium	64:133	A Gram-stain-positive, aerobic, motile, rod- or cocci-shaped bacterium with flagella bacterium (THG-T63T)	64:168	A Gram-stain-positive, aerobic, motile, rod- or cocci-shaped bacterium with flagella bacterium (THG-T63T) was isolated from freshwater mud.
29683418	14	62	theme	19192T=CGMCC	1562:1573	arg1	4.7388T					1575:1581	=KACC 19192T=CGMCC 4.7388T	1556:1581	=KACC 19192T=CGMCC 4.7388T	1556:1581	The type strain is THG-T63T(=KACC 19192T=CGMCC 4.7388T).
29683418	14	62	theme	19192T=CGMCC	1562:1573	arg1	THG-T63T					1547:1554	THG-T63T	1547:1554	THG-T63T(=KACC 19192T=CGMCC 4.7388T)	1547:1582	The type strain is THG-T63T(=KACC 19192T=CGMCC 4.7388T).
29683418	11	63	theme	DNA-DNA	1153:1159	arg1	relatedness					1161:1171	DNA-DNA relatedness	1153:1171	DNA-DNA relatedness of 20-44 % to the type strains of phylogenetically related Nocardioides species	1153:1251	Strain THG-T63T exhibited levels of DNA-DNA relatedness of 20-44 % to the type strains of phylogenetically related Nocardioides species and could be differentiated from these species based on differences in phenotypic characteristics.
29683418	12	64	theme	name	1477:1480	arg1	sp					1506:1507	the name Nocardioides pelophilus sp	1473:1507	the name Nocardioides pelophilus sp	1473:1507	On the basis of the data presented here, strain THG-T63T represents a novel species of the genus Nocardioides, for which the name Nocardioides pelophilus sp.
29683418	12	65	theme	Nocardioides	1449:1460	arg1	species					1428:1434	a novel species	1420:1434	a novel species	1420:1434	On the basis of the data presented here, strain THG-T63T represents a novel species of the genus Nocardioides, for which the name Nocardioides pelophilus sp.
29683418	4	66	theme	rRNA	323:326	arg1	analysis					337:344	16S rRNA sequence analysis	319:344	16S rRNA sequence analysis	319:344	Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T63T were identified as Nocardioides panacisoli KCTC 19470T (97.5 %), Nocardioides caeni KCTC 19600T (96.4 %), Nocardioides humi KCTC 19265T (96.3 %), Nocardioides kongjuensis KCTC 19054T (96.1 %) and Nocardioides nitrophenolicus KCTC 457BPT (96.1 %).
29683418	4	67	theme	Nocardioides	598:609	arg1	neighbours					372:381	the nearest phylogenetic neighbours	347:381	the nearest phylogenetic neighbours of strain THG-T63T	347:400	Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T63T were identified as Nocardioides panacisoli KCTC 19470T (97.5 %), Nocardioides caeni KCTC 19600T (96.4 %), Nocardioides humi KCTC 19265T (96.3 %), Nocardioides kongjuensis KCTC 19054T (96.1 %) and Nocardioides nitrophenolicus KCTC 457BPT (96.1 %).
29683418	4	67	theme	Nocardioides	598:609	arg1	457BPT					632:637	Nocardioides nitrophenolicus KCTC 457BPT	598:637	Nocardioides nitrophenolicus KCTC 457BPT (96.1 %)	598:646	Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T63T were identified as Nocardioides panacisoli KCTC 19470T (97.5 %), Nocardioides caeni KCTC 19600T (96.4 %), Nocardioides humi KCTC 19265T (96.3 %), Nocardioides kongjuensis KCTC 19054T (96.1 %) and Nocardioides nitrophenolicus KCTC 457BPT (96.1 %).
29683418	4	67	theme	Nocardioides	598:609	arg1	%					645:645	96.1 %	640:645	96.1 %	640:645	Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T63T were identified as Nocardioides panacisoli KCTC 19470T (97.5 %), Nocardioides caeni KCTC 19600T (96.4 %), Nocardioides humi KCTC 19265T (96.3 %), Nocardioides kongjuensis KCTC 19054T (96.1 %) and Nocardioides nitrophenolicus KCTC 457BPT (96.1 %).
29683418	12	68	theme	novel	1422:1426	arg1	species					1428:1434	a novel species	1420:1434	a novel species	1420:1434	On the basis of the data presented here, strain THG-T63T represents a novel species of the genus Nocardioides, for which the name Nocardioides pelophilus sp.
29683418	4	69	theme	KCTC	573:576	arg1	%					591:591	96.1 %	586:591	96.1 %	586:591	Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T63T were identified as Nocardioides panacisoli KCTC 19470T (97.5 %), Nocardioides caeni KCTC 19600T (96.4 %), Nocardioides humi KCTC 19265T (96.3 %), Nocardioides kongjuensis KCTC 19054T (96.1 %) and Nocardioides nitrophenolicus KCTC 457BPT (96.1 %).
29683418	4	69	theme	KCTC	573:576	arg1	neighbours					372:381	the nearest phylogenetic neighbours	347:381	the nearest phylogenetic neighbours of strain THG-T63T	347:400	Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T63T were identified as Nocardioides panacisoli KCTC 19470T (97.5 %), Nocardioides caeni KCTC 19600T (96.4 %), Nocardioides humi KCTC 19265T (96.3 %), Nocardioides kongjuensis KCTC 19054T (96.1 %) and Nocardioides nitrophenolicus KCTC 457BPT (96.1 %).
29683418	4	69	theme	KCTC	573:576	arg1	19054T					578:583	Nocardioides kongjuensis KCTC 19054T	548:583	Nocardioides kongjuensis KCTC 19054T (96.1 %)	548:592	Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T63T were identified as Nocardioides panacisoli KCTC 19470T (97.5 %), Nocardioides caeni KCTC 19600T (96.4 %), Nocardioides humi KCTC 19265T (96.3 %), Nocardioides kongjuensis KCTC 19054T (96.1 %) and Nocardioides nitrophenolicus KCTC 457BPT (96.1 %).
29683418	14	70	theme	=KACC	1556:1560	arg1	4.7388T					1575:1581	=KACC 19192T=CGMCC 4.7388T	1556:1581	=KACC 19192T=CGMCC 4.7388T	1556:1581	The type strain is THG-T63T(=KACC 19192T=CGMCC 4.7388T).
29683418	14	70	theme	=KACC	1556:1560	arg1	THG-T63T					1547:1554	THG-T63T	1547:1554	THG-T63T(=KACC 19192T=CGMCC 4.7388T)	1547:1582	The type strain is THG-T63T(=KACC 19192T=CGMCC 4.7388T).
29683418	4	71	theme	KCTC	486:489	arg1	neighbours					372:381	the nearest phylogenetic neighbours	347:381	the nearest phylogenetic neighbours of strain THG-T63T	347:400	Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T63T were identified as Nocardioides panacisoli KCTC 19470T (97.5 %), Nocardioides caeni KCTC 19600T (96.4 %), Nocardioides humi KCTC 19265T (96.3 %), Nocardioides kongjuensis KCTC 19054T (96.1 %) and Nocardioides nitrophenolicus KCTC 457BPT (96.1 %).
29683418	4	71	theme	KCTC	486:489	arg1	19600T					491:496	Nocardioides caeni KCTC 19600T	467:496	Nocardioides caeni KCTC 19600T (96.4 %)	467:505	Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T63T were identified as Nocardioides panacisoli KCTC 19470T (97.5 %), Nocardioides caeni KCTC 19600T (96.4 %), Nocardioides humi KCTC 19265T (96.3 %), Nocardioides kongjuensis KCTC 19054T (96.1 %) and Nocardioides nitrophenolicus KCTC 457BPT (96.1 %).
29683418	4	71	theme	KCTC	486:489	arg1	%					504:504	96.4 %	499:504	96.4 %	499:504	Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T63T were identified as Nocardioides panacisoli KCTC 19470T (97.5 %), Nocardioides caeni KCTC 19600T (96.4 %), Nocardioides humi KCTC 19265T (96.3 %), Nocardioides kongjuensis KCTC 19054T (96.1 %) and Nocardioides nitrophenolicus KCTC 457BPT (96.1 %).
29683418	11	72	theme	relatedness	1161:1171	arg1	levels					1143:1148	levels	1143:1148	levels of DNA-DNA relatedness of 20-44 % to the type strains of phylogenetically related Nocardioides species	1143:1251	Strain THG-T63T exhibited levels of DNA-DNA relatedness of 20-44 % to the type strains of phylogenetically related Nocardioides species and could be differentiated from these species based on differences in phenotypic characteristics.
29683418	4	73	theme	16S	319:321	arg1	analysis					337:344	16S rRNA sequence analysis	319:344	16S rRNA sequence analysis	319:344	Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T63T were identified as Nocardioides panacisoli KCTC 19470T (97.5 %), Nocardioides caeni KCTC 19600T (96.4 %), Nocardioides humi KCTC 19265T (96.3 %), Nocardioides kongjuensis KCTC 19054T (96.1 %) and Nocardioides nitrophenolicus KCTC 457BPT (96.1 %).
29683418	5	74	theme	sequence	658:665	arg1	similarities					667:678	16S rRNA sequence similarities	649:678	16S rRNA sequence similarities among strain THG-T63T and other species	649:718	16S rRNA sequence similarities among strain THG-T63T and other species were lower than 96.0 %.
29683418	11	75	from	differences	1309:1319	arg1	characteristics					1335:1349	phenotypic characteristics	1324:1349	phenotypic characteristics	1324:1349	Strain THG-T63T exhibited levels of DNA-DNA relatedness of 20-44 % to the type strains of phylogenetically related Nocardioides species and could be differentiated from these species based on differences in phenotypic characteristics.
29683418	12	76	dep	data	1372:1375	arg1	the					1355:1357	the	1355:1357	the	1355:1357	On the basis of the data presented here, strain THG-T63T represents a novel species of the genus Nocardioides, for which the name Nocardioides pelophilus sp.
29683418	12	76	dep	data	1372:1375	arg1	basis					1359:1363	basis	1359:1363	basis	1359:1363	On the basis of the data presented here, strain THG-T63T represents a novel species of the genus Nocardioides, for which the name Nocardioides pelophilus sp.
29683418	4	77	theme	THG-T63T	393:400	arg1	19470T					450:455	Nocardioides panacisoli KCTC 19470T	421:455	Nocardioides panacisoli KCTC 19470T (97.5 %)	421:464	Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T63T were identified as Nocardioides panacisoli KCTC 19470T (97.5 %), Nocardioides caeni KCTC 19600T (96.4 %), Nocardioides humi KCTC 19265T (96.3 %), Nocardioides kongjuensis KCTC 19054T (96.1 %) and Nocardioides nitrophenolicus KCTC 457BPT (96.1 %).
29683418	4	77	theme	THG-T63T	393:400	arg1	19054T					578:583	Nocardioides kongjuensis KCTC 19054T	548:583	Nocardioides kongjuensis KCTC 19054T (96.1 %)	548:592	Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T63T were identified as Nocardioides panacisoli KCTC 19470T (97.5 %), Nocardioides caeni KCTC 19600T (96.4 %), Nocardioides humi KCTC 19265T (96.3 %), Nocardioides kongjuensis KCTC 19054T (96.1 %) and Nocardioides nitrophenolicus KCTC 457BPT (96.1 %).
29683418	4	77	theme	THG-T63T	393:400	arg1	neighbours					372:381	the nearest phylogenetic neighbours	347:381	the nearest phylogenetic neighbours of strain THG-T63T	347:400	Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T63T were identified as Nocardioides panacisoli KCTC 19470T (97.5 %), Nocardioides caeni KCTC 19600T (96.4 %), Nocardioides humi KCTC 19265T (96.3 %), Nocardioides kongjuensis KCTC 19054T (96.1 %) and Nocardioides nitrophenolicus KCTC 457BPT (96.1 %).
29683418	4	77	theme	THG-T63T	393:400	arg1	19600T					491:496	Nocardioides caeni KCTC 19600T	467:496	Nocardioides caeni KCTC 19600T (96.4 %)	467:505	Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T63T were identified as Nocardioides panacisoli KCTC 19470T (97.5 %), Nocardioides caeni KCTC 19600T (96.4 %), Nocardioides humi KCTC 19265T (96.3 %), Nocardioides kongjuensis KCTC 19054T (96.1 %) and Nocardioides nitrophenolicus KCTC 457BPT (96.1 %).
29683418	4	77	theme	THG-T63T	393:400	arg1	457BPT					632:637	Nocardioides nitrophenolicus KCTC 457BPT	598:637	Nocardioides nitrophenolicus KCTC 457BPT (96.1 %)	598:646	Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T63T were identified as Nocardioides panacisoli KCTC 19470T (97.5 %), Nocardioides caeni KCTC 19600T (96.4 %), Nocardioides humi KCTC 19265T (96.3 %), Nocardioides kongjuensis KCTC 19054T (96.1 %) and Nocardioides nitrophenolicus KCTC 457BPT (96.1 %).
29683418	4	77	theme	THG-T63T	393:400	arg1	19265T					531:536	Nocardioides humi KCTC 19265T	508:536	Nocardioides humi KCTC 19265T (96.3 %)	508:545	Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T63T were identified as Nocardioides panacisoli KCTC 19470T (97.5 %), Nocardioides caeni KCTC 19600T (96.4 %), Nocardioides humi KCTC 19265T (96.3 %), Nocardioides kongjuensis KCTC 19054T (96.1 %) and Nocardioides nitrophenolicus KCTC 457BPT (96.1 %).
29683418	3	78	theme	%	287:287	arg1	optimum					295:301	optimum	295:301	optimum	295:301	Growth occurred at 10-40 °C (optimum, 28-35 °C), at pH 6-8 (optimum, 7) and at 0-6 % NaCl (optimum, 2 %).
29683418	3	78	theme	%	287:287	arg1	 NaCl					288:292	0-6 % NaCl	283:292	0-6 % NaCl (optimum, 2 %)	283:307	Growth occurred at 10-40 °C (optimum, 28-35 °C), at pH 6-8 (optimum, 7) and at 0-6 % NaCl (optimum, 2 %).
29676718	12	0	theme	15566T=LMG	1165:1174	arg1	29810T					1176:1181	=CCTCC AB 2016249T=KCTC 15566T=LMG 29810T	1141:1181	=CCTCC AB 2016249T=KCTC 15566T=LMG 29810T	1141:1181	The type strain is Y1A-10 4-9-1T (=CCTCC AB 2016249T=KCTC 15566T=LMG 29810T).
29676718	12	0	theme	15566T=LMG	1165:1174	arg1	4-9-1T					1133:1138	Y1A-10 4-9-1T	1126:1138	Y1A-10 4-9-1T (=CCTCC AB 2016249T=KCTC 15566T=LMG 29810T)	1126:1182	The type strain is Y1A-10 4-9-1T (=CCTCC AB 2016249T=KCTC 15566T=LMG 29810T).
29676718	7	1	theme	predominant	603:613	arg1	MK-9					631:634	MK-9	631:634	MK-9(H4)	631:638	The predominant menaquinone was MK-9(H4) and meso-diaminopimelic acid was present in the cell-wall peptidoglycan layer.
29676718	7	1	theme	predominant	603:613	arg1	menaquinone					615:625	The predominant menaquinone	599:625	The predominant menaquinone	599:625	The predominant menaquinone was MK-9(H4) and meso-diaminopimelic acid was present in the cell-wall peptidoglycan layer.
29676718	12	2	theme	2016249T=KCTC	1151:1163	arg1	29810T					1176:1181	=CCTCC AB 2016249T=KCTC 15566T=LMG 29810T	1141:1181	=CCTCC AB 2016249T=KCTC 15566T=LMG 29810T	1141:1181	The type strain is Y1A-10 4-9-1T (=CCTCC AB 2016249T=KCTC 15566T=LMG 29810T).
29676718	12	2	theme	2016249T=KCTC	1151:1163	arg1	4-9-1T					1133:1138	Y1A-10 4-9-1T	1126:1138	Y1A-10 4-9-1T (=CCTCC AB 2016249T=KCTC 15566T=LMG 29810T)	1126:1182	The type strain is Y1A-10 4-9-1T (=CCTCC AB 2016249T=KCTC 15566T=LMG 29810T).
29676718	7	3	located	present	673:679	arg2	acid					664:667	meso-diaminopimelic acid	644:667	meso-diaminopimelic acid	644:667	The predominant menaquinone was MK-9(H4) and meso-diaminopimelic acid was present in the cell-wall peptidoglycan layer.
29676718	7	3	located	present	673:679	arg1	layer					712:716	the cell-wall peptidoglycan layer	684:716	the cell-wall peptidoglycan layer	684:716	The predominant menaquinone was MK-9(H4) and meso-diaminopimelic acid was present in the cell-wall peptidoglycan layer.
29676718	10	4	theme	strain	966:971	arg1	4-9-1T					980:985	strain Y1A-10 4-9-1T	966:985	strain Y1A-10 4-9-1T	966:985	The phylogenetic and phenotypic results supported that strain Y1A-10 4-9-1T is a novel species of the genus Propionicimonas, for which the name Propionicimonas ferrireducens sp.
29676718	10	4	theme	strain	966:971	arg1	species					998:1004	a novel species	990:1004	a novel species	990:1004	The phylogenetic and phenotypic results supported that strain Y1A-10 4-9-1T is a novel species of the genus Propionicimonas, for which the name Propionicimonas ferrireducens sp.
29676718	1	5	theme	dissimilatory	54:66	arg1	enrichment					97:106	dissimilatory iron(III)-reducing microbial enrichment	54:106	dissimilatory iron(III)-reducing microbial enrichment obtained from paddy soil	54:131	nov., isolated from dissimilatory iron(III)-reducing microbial enrichment obtained from paddy soil.
29676718	6	6	theme	DNA	568:570	arg1	G+C content					572:582	The genomic DNA G+C content	556:582	The genomic DNA G+C content	556:582	The genomic DNA G+C content was 63.9 mol%.
29676718	6	6	theme	DNA	568:570	arg1	%					596:596	63.9 mol%	588:596	63.9 mol%	588:596	The genomic DNA G+C content was 63.9 mol%.
29676718	9	7	dep	anteiso-C15 	878:889	arg1	 0					907:908	 0	907:908	 0	907:908	The dominant cellular fatty acids detected were anteiso-C15 : 0 and iso-C16 : 0.
29676718	9	7	dep	anteiso-C15 	878:889	arg1	iso-C16 					898:905	iso-C16 	898:905	iso-C16 	898:905	The dominant cellular fatty acids detected were anteiso-C15 : 0 and iso-C16 : 0.
29676718	9	7	dep	anteiso-C15 	878:889	arg1	 0					891:892	 0	891:892	 0	891:892	The dominant cellular fatty acids detected were anteiso-C15 : 0 and iso-C16 : 0.
29676718	1	8	theme	iron	68:71	arg1	enrichment					97:106	dissimilatory iron(III)-reducing microbial enrichment	54:106	dissimilatory iron(III)-reducing microbial enrichment obtained from paddy soil	54:131	nov., isolated from dissimilatory iron(III)-reducing microbial enrichment obtained from paddy soil.
29676718	4	9	theme	optimal	378:384	arg1	7.0					393:395	7.0	393:395	7.0	393:395	The optimal growth temperature was 30 °C and the optimal pH was 7.0.
29676718	4	9	theme	optimal	378:384	arg1	pH					386:387	the optimal pH	374:387	the optimal pH	374:387	The optimal growth temperature was 30 °C and the optimal pH was 7.0.
29676718	2	10	from	soil	247:250	arg1	China					273:277	China	273:277	China	273:277	A novel strain, designated Y1A-10 4-9-1T, with Gram-stain-positive and rod-shaped cells, was isolated from paddy soil in Yingtan, Jiangxi, China.
29676718	2	10	from	soil	247:250	arg1	Jiangxi					264:270	Jiangxi	264:270	Jiangxi	264:270	A novel strain, designated Y1A-10 4-9-1T, with Gram-stain-positive and rod-shaped cells, was isolated from paddy soil in Yingtan, Jiangxi, China.
29676718	2	11	theme	novel	136:140	arg1	strain					142:147	A novel strain	134:147	A novel strain	134:147	A novel strain, designated Y1A-10 4-9-1T, with Gram-stain-positive and rod-shaped cells, was isolated from paddy soil in Yingtan, Jiangxi, China.
29676718	12	12	theme	Y1A-10	1126:1131	arg1	strain					1116:1121	The type strain	1107:1121	The type strain	1107:1121	The type strain is Y1A-10 4-9-1T (=CCTCC AB 2016249T=KCTC 15566T=LMG 29810T).
29676718	12	12	theme	Y1A-10	1126:1131	arg1	4-9-1T					1133:1138	Y1A-10 4-9-1T	1126:1138	Y1A-10 4-9-1T (=CCTCC AB 2016249T=KCTC 15566T=LMG 29810T)	1126:1182	The type strain is Y1A-10 4-9-1T (=CCTCC AB 2016249T=KCTC 15566T=LMG 29810T).
29676718	12	12	theme	Y1A-10	1126:1131	arg1	29810T					1176:1181	=CCTCC AB 2016249T=KCTC 15566T=LMG 29810T	1141:1181	=CCTCC AB 2016249T=KCTC 15566T=LMG 29810T	1141:1181	The type strain is Y1A-10 4-9-1T (=CCTCC AB 2016249T=KCTC 15566T=LMG 29810T).
29676718	10	13	theme	name	1050:1053	arg1	Propionicimonas					1055:1069	the name Propionicimonas	1046:1069	the name Propionicimonas	1046:1069	The phylogenetic and phenotypic results supported that strain Y1A-10 4-9-1T is a novel species of the genus Propionicimonas, for which the name Propionicimonas ferrireducens sp.
29676718	4	14	theme	growth	341:346	arg1	temperature					348:358	The optimal growth temperature	329:358	The optimal growth temperature	329:358	The optimal growth temperature was 30 °C and the optimal pH was 7.0.
29676718	6	15	theme	genomic	560:566	arg1	G+C content					572:582	The genomic DNA G+C content	556:582	The genomic DNA G+C content	556:582	The genomic DNA G+C content was 63.9 mol%.
29676718	6	15	theme	genomic	560:566	arg1	%					596:596	63.9 mol%	588:596	63.9 mol%	588:596	The genomic DNA G+C content was 63.9 mol%.
29676718	4	16	theme	optimal	333:339	arg1	temperature					348:358	The optimal growth temperature	329:358	The optimal growth temperature	329:358	The optimal growth temperature was 30 °C and the optimal pH was 7.0.
29676718	10	17	theme	phenotypic	932:941	arg1	results					943:949	The phylogenetic and phenotypic results	911:949	The phylogenetic and phenotypic results	911:949	The phylogenetic and phenotypic results supported that strain Y1A-10 4-9-1T is a novel species of the genus Propionicimonas, for which the name Propionicimonas ferrireducens sp.
29676718	5	18	theme	novel	472:476	arg1	strain					478:483	the novel strain	468:483	the novel strain	468:483	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the novel strain is closely related to Propionicimonas paludicola JCM 11933T (98.57 %).
29676718	5	18	theme	novel	472:476	arg1	related					496:502	related	496:502	related	496:502	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the novel strain is closely related to Propionicimonas paludicola JCM 11933T (98.57 %).
29676718	7	19	from	present	673:679	arg1	layer					712:716	the cell-wall peptidoglycan layer	684:716	the cell-wall peptidoglycan layer	684:716	The predominant menaquinone was MK-9(H4) and meso-diaminopimelic acid was present in the cell-wall peptidoglycan layer.
29676718	1	20	theme	-reducing	77:85	arg1	enrichment					97:106	dissimilatory iron(III)-reducing microbial enrichment	54:106	dissimilatory iron(III)-reducing microbial enrichment obtained from paddy soil	54:131	nov., isolated from dissimilatory iron(III)-reducing microbial enrichment obtained from paddy soil.
29676718	5	21	theme	16S	429:431	arg1	sequences					443:451	16S rRNA gene sequences	429:451	16S rRNA gene sequences	429:451	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the novel strain is closely related to Propionicimonas paludicola JCM 11933T (98.57 %).
29676718	7	22	theme	meso-diaminopimelic	644:662	arg1	acid					664:667	meso-diaminopimelic acid	644:667	meso-diaminopimelic acid	644:667	The predominant menaquinone was MK-9(H4) and meso-diaminopimelic acid was present in the cell-wall peptidoglycan layer.
29676718	12	23	theme	type	1111:1114	arg1	strain					1116:1121	The type strain	1107:1121	The type strain	1107:1121	The type strain is Y1A-10 4-9-1T (=CCTCC AB 2016249T=KCTC 15566T=LMG 29810T).
29676718	12	23	theme	type	1111:1114	arg1	4-9-1T					1133:1138	Y1A-10 4-9-1T	1126:1138	Y1A-10 4-9-1T (=CCTCC AB 2016249T=KCTC 15566T=LMG 29810T)	1126:1182	The type strain is Y1A-10 4-9-1T (=CCTCC AB 2016249T=KCTC 15566T=LMG 29810T).
29676718	10	24	theme	phylogenetic	915:926	arg1	results					943:949	The phylogenetic and phenotypic results	911:949	The phylogenetic and phenotypic results	911:949	The phylogenetic and phenotypic results supported that strain Y1A-10 4-9-1T is a novel species of the genus Propionicimonas, for which the name Propionicimonas ferrireducens sp.
29676718	1	25	theme	microbial	87:95	arg1	enrichment					97:106	dissimilatory iron(III)-reducing microbial enrichment	54:106	dissimilatory iron(III)-reducing microbial enrichment obtained from paddy soil	54:131	nov., isolated from dissimilatory iron(III)-reducing microbial enrichment obtained from paddy soil.
29676718	5	26	theme	rRNA	433:436	arg1	sequences					443:451	16S rRNA gene sequences	429:451	16S rRNA gene sequences	429:451	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the novel strain is closely related to Propionicimonas paludicola JCM 11933T (98.57 %).
29676718	9	27	theme	dominant	834:841	arg1	acids					858:862	The dominant cellular fatty acids	830:862	The dominant cellular fatty acids detected	830:871	The dominant cellular fatty acids detected were anteiso-C15 : 0 and iso-C16 : 0.
29676718	9	27	theme	dominant	834:841	arg1	anteiso-C15 					878:889	anteiso-C15 	878:889	anteiso-C15 	878:889	The dominant cellular fatty acids detected were anteiso-C15 : 0 and iso-C16 : 0.
29676718	10	28	theme	novel	992:996	arg1	4-9-1T					980:985	strain Y1A-10 4-9-1T	966:985	strain Y1A-10 4-9-1T	966:985	The phylogenetic and phenotypic results supported that strain Y1A-10 4-9-1T is a novel species of the genus Propionicimonas, for which the name Propionicimonas ferrireducens sp.
29676718	10	28	theme	novel	992:996	arg1	species					998:1004	a novel species	990:1004	a novel species	990:1004	The phylogenetic and phenotypic results supported that strain Y1A-10 4-9-1T is a novel species of the genus Propionicimonas, for which the name Propionicimonas ferrireducens sp.
29676718	5	29	theme	gene	438:441	arg1	sequences					443:451	16S rRNA gene sequences	429:451	16S rRNA gene sequences	429:451	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the novel strain is closely related to Propionicimonas paludicola JCM 11933T (98.57 %).
29676718	3	30	dep	wide	303:306	arg1	long					323:326	long	323:326	long	323:326	Cells were 0.15-0.2 µm wide and 1.5-3.3 µm long.
29676718	2	31	theme	Y1A-10	161:166	arg1	4-9-1T					168:173	Y1A-10 4-9-1T	161:173	Y1A-10 4-9-1T	161:173	A novel strain, designated Y1A-10 4-9-1T, with Gram-stain-positive and rod-shaped cells, was isolated from paddy soil in Yingtan, Jiangxi, China.
29676718	8	32	theme	unidentified	775:786	arg1	phospholipid					788:799	one unidentified phospholipid	771:799	one unidentified phospholipid	771:799	The major polar lipids were diphosphatidylglycerol, one unidentified phospholipid and two unidentified lipids.
29676718	9	33	theme	fatty	852:856	arg1	acids					858:862	The dominant cellular fatty acids	830:862	The dominant cellular fatty acids detected	830:871	The dominant cellular fatty acids detected were anteiso-C15 : 0 and iso-C16 : 0.
29676718	9	33	theme	fatty	852:856	arg1	anteiso-C15 					878:889	anteiso-C15 	878:889	anteiso-C15 	878:889	The dominant cellular fatty acids detected were anteiso-C15 : 0 and iso-C16 : 0.
29676718	7	34	attach	present	673:679	arg2	acid					664:667	meso-diaminopimelic acid	644:667	meso-diaminopimelic acid	644:667	The predominant menaquinone was MK-9(H4) and meso-diaminopimelic acid was present in the cell-wall peptidoglycan layer.
29676718	7	34	attach	present	673:679	arg1	layer					712:716	the cell-wall peptidoglycan layer	684:716	the cell-wall peptidoglycan layer	684:716	The predominant menaquinone was MK-9(H4) and meso-diaminopimelic acid was present in the cell-wall peptidoglycan layer.
29676718	5	35	theme	Propionicimonas	507:521	arg1	11933T					538:543	Propionicimonas paludicola JCM 11933T	507:543	Propionicimonas paludicola JCM 11933T (98.57 %)	507:553	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the novel strain is closely related to Propionicimonas paludicola JCM 11933T (98.57 %).
29676718	5	35	theme	Propionicimonas	507:521	arg1	%					552:552	98.57 %	546:552	98.57 %	546:552	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the novel strain is closely related to Propionicimonas paludicola JCM 11933T (98.57 %).
29676718	10	36	theme	Propionicimonas	1019:1033	arg1	4-9-1T					980:985	strain Y1A-10 4-9-1T	966:985	strain Y1A-10 4-9-1T	966:985	The phylogenetic and phenotypic results supported that strain Y1A-10 4-9-1T is a novel species of the genus Propionicimonas, for which the name Propionicimonas ferrireducens sp.
29676718	10	36	theme	Propionicimonas	1019:1033	arg1	species					998:1004	a novel species	990:1004	a novel species	990:1004	The phylogenetic and phenotypic results supported that strain Y1A-10 4-9-1T is a novel species of the genus Propionicimonas, for which the name Propionicimonas ferrireducens sp.
29676718	10	37	theme	genus	1013:1017	arg1	Propionicimonas					1019:1033	the genus Propionicimonas	1009:1033	the genus Propionicimonas	1009:1033	The phylogenetic and phenotypic results supported that strain Y1A-10 4-9-1T is a novel species of the genus Propionicimonas, for which the name Propionicimonas ferrireducens sp.
29676718	2	38	theme	rod-shaped	205:214	arg1	cells					216:220	Gram-stain-positive and rod-shaped cells	181:220	Gram-stain-positive and rod-shaped cells	181:220	A novel strain, designated Y1A-10 4-9-1T, with Gram-stain-positive and rod-shaped cells, was isolated from paddy soil in Yingtan, Jiangxi, China.
29676718	5	39	theme	paludicola	523:532	arg1	11933T					538:543	Propionicimonas paludicola JCM 11933T	507:543	Propionicimonas paludicola JCM 11933T (98.57 %)	507:553	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the novel strain is closely related to Propionicimonas paludicola JCM 11933T (98.57 %).
29676718	5	39	theme	paludicola	523:532	arg1	%					552:552	98.57 %	546:552	98.57 %	546:552	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the novel strain is closely related to Propionicimonas paludicola JCM 11933T (98.57 %).
29676718	5	40	theme	Phylogenetic	398:409	arg1	analysis					411:418	Phylogenetic analysis	398:418	Phylogenetic analysis based on 16S rRNA gene sequences	398:451	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the novel strain is closely related to Propionicimonas paludicola JCM 11933T (98.57 %).
29676718	2	41	theme	Gram-stain-positive	181:199	arg1	cells					216:220	Gram-stain-positive and rod-shaped cells	181:220	Gram-stain-positive and rod-shaped cells	181:220	A novel strain, designated Y1A-10 4-9-1T, with Gram-stain-positive and rod-shaped cells, was isolated from paddy soil in Yingtan, Jiangxi, China.
29676718	10	42	theme	Y1A-10	973:978	arg1	4-9-1T					980:985	strain Y1A-10 4-9-1T	966:985	strain Y1A-10 4-9-1T	966:985	The phylogenetic and phenotypic results supported that strain Y1A-10 4-9-1T is a novel species of the genus Propionicimonas, for which the name Propionicimonas ferrireducens sp.
29676718	10	42	theme	Y1A-10	973:978	arg1	species					998:1004	a novel species	990:1004	a novel species	990:1004	The phylogenetic and phenotypic results supported that strain Y1A-10 4-9-1T is a novel species of the genus Propionicimonas, for which the name Propionicimonas ferrireducens sp.
29676718	5	43	theme	JCM	534:536	arg1	11933T					538:543	Propionicimonas paludicola JCM 11933T	507:543	Propionicimonas paludicola JCM 11933T (98.57 %)	507:553	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the novel strain is closely related to Propionicimonas paludicola JCM 11933T (98.57 %).
29676718	5	43	theme	JCM	534:536	arg1	%					552:552	98.57 %	546:552	98.57 %	546:552	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the novel strain is closely related to Propionicimonas paludicola JCM 11933T (98.57 %).
29676718	1	44	theme	paddy	122:126	arg1	soil					128:131	paddy soil	122:131	paddy soil	122:131	nov., isolated from dissimilatory iron(III)-reducing microbial enrichment obtained from paddy soil.
29676718	7	45	theme	peptidoglycan	698:710	arg1	layer					712:716	the cell-wall peptidoglycan layer	684:716	the cell-wall peptidoglycan layer	684:716	The predominant menaquinone was MK-9(H4) and meso-diaminopimelic acid was present in the cell-wall peptidoglycan layer.
29676718	7	46	from	layer	712:716	arg1	present					673:679	present	673:679	present	673:679	The predominant menaquinone was MK-9(H4) and meso-diaminopimelic acid was present in the cell-wall peptidoglycan layer.
29676718	9	47	theme	cellular	843:850	arg1	acids					858:862	The dominant cellular fatty acids	830:862	The dominant cellular fatty acids detected	830:871	The dominant cellular fatty acids detected were anteiso-C15 : 0 and iso-C16 : 0.
29676718	9	47	theme	cellular	843:850	arg1	anteiso-C15 					878:889	anteiso-C15 	878:889	anteiso-C15 	878:889	The dominant cellular fatty acids detected were anteiso-C15 : 0 and iso-C16 : 0.
29676718	2	48	theme	paddy	241:245	arg1	soil					247:250	paddy soil	241:250	paddy soil in Yingtan, Jiangxi, China	241:277	A novel strain, designated Y1A-10 4-9-1T, with Gram-stain-positive and rod-shaped cells, was isolated from paddy soil in Yingtan, Jiangxi, China.
29676718	12	49	theme	=CCTCC AB	1141:1149	arg1	29810T					1176:1181	=CCTCC AB 2016249T=KCTC 15566T=LMG 29810T	1141:1181	=CCTCC AB 2016249T=KCTC 15566T=LMG 29810T	1141:1181	The type strain is Y1A-10 4-9-1T (=CCTCC AB 2016249T=KCTC 15566T=LMG 29810T).
29676718	12	49	theme	=CCTCC AB	1141:1149	arg1	4-9-1T					1133:1138	Y1A-10 4-9-1T	1126:1138	Y1A-10 4-9-1T (=CCTCC AB 2016249T=KCTC 15566T=LMG 29810T)	1126:1182	The type strain is Y1A-10 4-9-1T (=CCTCC AB 2016249T=KCTC 15566T=LMG 29810T).
29676718	8	50	theme	unidentified	809:820	arg1	lipids					822:827	two unidentified lipids	805:827	two unidentified lipids	805:827	The major polar lipids were diphosphatidylglycerol, one unidentified phospholipid and two unidentified lipids.
29676718	2	51	attach	isolated	227:234	arg2	strain					142:147	A novel strain	134:147	A novel strain	134:147	A novel strain, designated Y1A-10 4-9-1T, with Gram-stain-positive and rod-shaped cells, was isolated from paddy soil in Yingtan, Jiangxi, China.
29676718	2	51	attach	isolated	227:234	arg1	soil					247:250	paddy soil	241:250	paddy soil in Yingtan, Jiangxi, China	241:277	A novel strain, designated Y1A-10 4-9-1T, with Gram-stain-positive and rod-shaped cells, was isolated from paddy soil in Yingtan, Jiangxi, China.
29676718	8	52	theme	polar	729:733	arg1	diphosphatidylglycerol					747:768	diphosphatidylglycerol	747:768	diphosphatidylglycerol	747:768	The major polar lipids were diphosphatidylglycerol, one unidentified phospholipid and two unidentified lipids.
29676718	8	52	theme	polar	729:733	arg1	lipids					735:740	The major polar lipids	719:740	The major polar lipids	719:740	The major polar lipids were diphosphatidylglycerol, one unidentified phospholipid and two unidentified lipids.
29676718	8	53	theme	major	723:727	arg1	diphosphatidylglycerol					747:768	diphosphatidylglycerol	747:768	diphosphatidylglycerol	747:768	The major polar lipids were diphosphatidylglycerol, one unidentified phospholipid and two unidentified lipids.
29676718	8	53	theme	major	723:727	arg1	lipids					735:740	The major polar lipids	719:740	The major polar lipids	719:740	The major polar lipids were diphosphatidylglycerol, one unidentified phospholipid and two unidentified lipids.
29676718	6	54	theme	63.9 mol	588:595	arg1	G+C content					572:582	The genomic DNA G+C content	556:582	The genomic DNA G+C content	556:582	The genomic DNA G+C content was 63.9 mol%.
29676718	6	54	theme	63.9 mol	588:595	arg1	%					596:596	63.9 mol%	588:596	63.9 mol%	588:596	The genomic DNA G+C content was 63.9 mol%.
29676718	7	55	theme	cell-wall	688:696	arg1	layer					712:716	the cell-wall peptidoglycan layer	684:716	the cell-wall peptidoglycan layer	684:716	The predominant menaquinone was MK-9(H4) and meso-diaminopimelic acid was present in the cell-wall peptidoglycan layer.
29458475	4	0	theme	Phylogenetic	420:431	arg1	analysis					433:440	Phylogenetic analysis	420:440	Phylogenetic analysis based on 16S rRNA gene sequences	420:473	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain LCB256T was most closely related to the two genera of Ornithinibacillus and Oceanobacillus, showing highest sequence similarity to Oceanobacillus limi KCTC 13823T (97.8 %) and Ornithinibacillus bavariensis WSBC 24001T (97.2 %).
29458475	10	1	theme	Ornithinibacillussalinisoli	1377:1403	arg1	sp					1405:1406	the genus Ornithinibacillus, Ornithinibacillussalinisoli sp	1348:1406	the genus Ornithinibacillus, Ornithinibacillussalinisoli sp	1348:1406	Based on this polyphasic taxonomic study, a novel species of the genus Ornithinibacillus, Ornithinibacillussalinisoli sp.
29458475	4	2	theme	limi	643:646	arg1	13823T					653:658	Oceanobacillus limi KCTC 13823T	628:658	Oceanobacillus limi KCTC 13823T (97.8 %)	628:667	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain LCB256T was most closely related to the two genera of Ornithinibacillus and Oceanobacillus, showing highest sequence similarity to Oceanobacillus limi KCTC 13823T (97.8 %) and Ornithinibacillus bavariensis WSBC 24001T (97.2 %).
29458475	4	2	theme	limi	643:646	arg1	%					666:666	97.8 %	661:666	97.8 %	661:666	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain LCB256T was most closely related to the two genera of Ornithinibacillus and Oceanobacillus, showing highest sequence similarity to Oceanobacillus limi KCTC 13823T (97.8 %) and Ornithinibacillus bavariensis WSBC 24001T (97.2 %).
29458475	12	3	theme	1.15809T=KCTC	1462:1474	arg1	33862T					1476:1481	=CGMCC 1.15809T=KCTC 33862T	1455:1481	=CGMCC 1.15809T=KCTC 33862T	1455:1481	The type strain is LCB256T (=CGMCC 1.15809T=KCTC 33862T).
29458475	12	3	theme	1.15809T=KCTC	1462:1474	arg1	LCB256T					1446:1452	LCB256T	1446:1452	LCB256T (=CGMCC 1.15809T=KCTC 33862T)	1446:1482	The type strain is LCB256T (=CGMCC 1.15809T=KCTC 33862T).
29458475	3	4	dep	Gram-stain-positive	272:290	arg1	aerobic					293:299	aerobic	293:299	aerobic	293:299	Cells of strain LCB256T were Gram-stain-positive, aerobic, rod-shaped and grew at 3-17 % (w/v) NaCl (optimum 10-15 %), 10-52 °C (optimum 25-30 °C) and pH 7.0-9.0 (optimum 8.0).
29458475	3	4	dep	Gram-stain-positive	272:290	arg1	rod-shaped					302:311	rod-shaped	302:311	rod-shaped	302:311	Cells of strain LCB256T were Gram-stain-positive, aerobic, rod-shaped and grew at 3-17 % (w/v) NaCl (optimum 10-15 %), 10-52 °C (optimum 25-30 °C) and pH 7.0-9.0 (optimum 8.0).
29458475	2	5	theme	taxonomic	111:119	arg1	study					121:125	A taxonomic study	109:125	A taxonomic study	109:125	A taxonomic study was performed on strain LCB256T, which was isolated from a saline-alkali soil sample taken from northwestern China.
29458475	4	6	theme	Oceanobacillus	628:641	arg1	13823T					653:658	Oceanobacillus limi KCTC 13823T	628:658	Oceanobacillus limi KCTC 13823T (97.8 %)	628:667	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain LCB256T was most closely related to the two genera of Ornithinibacillus and Oceanobacillus, showing highest sequence similarity to Oceanobacillus limi KCTC 13823T (97.8 %) and Ornithinibacillus bavariensis WSBC 24001T (97.2 %).
29458475	4	6	theme	Oceanobacillus	628:641	arg1	%					666:666	97.8 %	661:666	97.8 %	661:666	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain LCB256T was most closely related to the two genera of Ornithinibacillus and Oceanobacillus, showing highest sequence similarity to Oceanobacillus limi KCTC 13823T (97.8 %) and Ornithinibacillus bavariensis WSBC 24001T (97.2 %).
29458475	12	7	theme	=CGMCC	1455:1460	arg1	33862T					1476:1481	=CGMCC 1.15809T=KCTC 33862T	1455:1481	=CGMCC 1.15809T=KCTC 33862T	1455:1481	The type strain is LCB256T (=CGMCC 1.15809T=KCTC 33862T).
29458475	12	7	theme	=CGMCC	1455:1460	arg1	LCB256T					1446:1452	LCB256T	1446:1452	LCB256T (=CGMCC 1.15809T=KCTC 33862T)	1446:1482	The type strain is LCB256T (=CGMCC 1.15809T=KCTC 33862T).
29458475	3	8	theme	w/v	333:335	arg1	NaCl					338:341	3-17 % (w/v) NaCl	325:341	3-17 % (w/v) NaCl (optimum 10-15 %)	325:359	Cells of strain LCB256T were Gram-stain-positive, aerobic, rod-shaped and grew at 3-17 % (w/v) NaCl (optimum 10-15 %), 10-52 °C (optimum 25-30 °C) and pH 7.0-9.0 (optimum 8.0).
29458475	3	8	theme	w/v	333:335	arg1	%					358:358	optimum 10-15 %	344:358	optimum 10-15 %	344:358	Cells of strain LCB256T were Gram-stain-positive, aerobic, rod-shaped and grew at 3-17 % (w/v) NaCl (optimum 10-15 %), 10-52 °C (optimum 25-30 °C) and pH 7.0-9.0 (optimum 8.0).
29458475	1	9	theme	halophilic	52:61	arg1	bacterium					63:71	a moderately halophilic bacterium	39:71	a moderately halophilic bacterium	39:71	nov., a moderately halophilic bacterium isolated from a saline-alkali soil.
29458475	1	9	theme	halophilic	52:61	arg1	nov.					33:36	nov.	33:36	nov.	33:36	nov., a moderately halophilic bacterium isolated from a saline-alkali soil.
29458475	9	10	theme	Ornithinibacillus	1174:1190	arg1	13822T					1208:1213	Ornithinibacillus halophilus KCTC 13822T	1174:1213	Ornithinibacillus halophilus KCTC 13822T	1174:1213	DNA-DNA relatedness values between strain LCB256T and Ornithinibacillus halophilus KCTC 13822T and Oceanobacillus limi KCTC 13823T were 46.2 and 34.8 %, respectively.
29458475	10	11	theme	genus	1352:1356	arg1	sp					1405:1406	the genus Ornithinibacillus, Ornithinibacillussalinisoli sp	1348:1406	the genus Ornithinibacillus, Ornithinibacillussalinisoli sp	1348:1406	Based on this polyphasic taxonomic study, a novel species of the genus Ornithinibacillus, Ornithinibacillussalinisoli sp.
29458475	3	12	theme	%	330:330	arg1	NaCl					338:341	3-17 % (w/v) NaCl	325:341	3-17 % (w/v) NaCl (optimum 10-15 %)	325:359	Cells of strain LCB256T were Gram-stain-positive, aerobic, rod-shaped and grew at 3-17 % (w/v) NaCl (optimum 10-15 %), 10-52 °C (optimum 25-30 °C) and pH 7.0-9.0 (optimum 8.0).
29458475	3	12	theme	%	330:330	arg1	%					358:358	optimum 10-15 %	344:358	optimum 10-15 %	344:358	Cells of strain LCB256T were Gram-stain-positive, aerobic, rod-shaped and grew at 3-17 % (w/v) NaCl (optimum 10-15 %), 10-52 °C (optimum 25-30 °C) and pH 7.0-9.0 (optimum 8.0).
29458475	2	13	theme	strain	144:149	arg1	LCB256T					151:157	strain LCB256T	144:157	strain LCB256T	144:157	A taxonomic study was performed on strain LCB256T, which was isolated from a saline-alkali soil sample taken from northwestern China.
29458475	6	14	theme	unidentified	976:987	arg1	aminolipids					989:999	two unidentified aminolipids	972:999	two unidentified aminolipids	972:999	The polar lipid profile of strain LCB256T contained diphosphatidylglycerol, phosphatidylglycerol, one unidentified phospholipid and two unidentified aminolipids.
29458475	8	15	theme	genomic	1093:1099	arg1	DNA					1101:1103	genomic DNA	1093:1103	genomic DNA	1093:1103	The G+C content of genomic DNA was 39.3 mol%.
29458475	9	16	theme	DNA-DNA	1120:1126	arg1	values					1140:1145	DNA-DNA relatedness values	1120:1145	DNA-DNA relatedness values between strain LCB256T and Ornithinibacillus halophilus KCTC 13822T and Oceanobacillus limi KCTC 13823T	1120:1249	DNA-DNA relatedness values between strain LCB256T and Ornithinibacillus halophilus KCTC 13822T and Oceanobacillus limi KCTC 13823T were 46.2 and 34.8 %, respectively.
29458475	0	17	theme	Ornithinibacillus	0:16	arg1	salinisoli					18:27	Ornithinibacillus salinisoli	0:27	Ornithinibacillus salinisoli	0:27	Ornithinibacillus salinisoli sp.
29458475	8	18	theme	DNA	1101:1103	arg1	%					1117:1117	39.3 mol%	1109:1117	39.3 mol%	1109:1117	The G+C content of genomic DNA was 39.3 mol%.
29458475	8	18	theme	DNA	1101:1103	arg1	G+C content					1078:1088	The G+C content	1074:1088	The G+C content of genomic DNA	1074:1103	The G+C content of genomic DNA was 39.3 mol%.
29458475	3	19	theme	optimum	344:350	arg1	NaCl					338:341	3-17 % (w/v) NaCl	325:341	3-17 % (w/v) NaCl (optimum 10-15 %)	325:359	Cells of strain LCB256T were Gram-stain-positive, aerobic, rod-shaped and grew at 3-17 % (w/v) NaCl (optimum 10-15 %), 10-52 °C (optimum 25-30 °C) and pH 7.0-9.0 (optimum 8.0).
29458475	3	19	theme	optimum	344:350	arg1	%					358:358	optimum 10-15 %	344:358	optimum 10-15 %	344:358	Cells of strain LCB256T were Gram-stain-positive, aerobic, rod-shaped and grew at 3-17 % (w/v) NaCl (optimum 10-15 %), 10-52 °C (optimum 25-30 °C) and pH 7.0-9.0 (optimum 8.0).
29458475	7	20	theme	fatty	1024:1028	arg1	anteiso-C15 					1041:1052	anteiso-C15 	1041:1052	anteiso-C15 	1041:1052	The dominant cellular fatty acids were anteiso-C15 : 0 and iso-C15 : 0.
29458475	7	20	theme	fatty	1024:1028	arg1	acids					1030:1034	The dominant cellular fatty acids	1002:1034	The dominant cellular fatty acids	1002:1034	The dominant cellular fatty acids were anteiso-C15 : 0 and iso-C15 : 0.
29458475	9	21	theme	relatedness	1128:1138	arg1	values					1140:1145	DNA-DNA relatedness values	1120:1145	DNA-DNA relatedness values between strain LCB256T and Ornithinibacillus halophilus KCTC 13822T and Oceanobacillus limi KCTC 13823T	1120:1249	DNA-DNA relatedness values between strain LCB256T and Ornithinibacillus halophilus KCTC 13822T and Oceanobacillus limi KCTC 13823T were 46.2 and 34.8 %, respectively.
29458475	6	22	contain	contained	882:890	arg2	phospholipid					955:966	one unidentified phospholipid	938:966	one unidentified phospholipid	938:966	The polar lipid profile of strain LCB256T contained diphosphatidylglycerol, phosphatidylglycerol, one unidentified phospholipid and two unidentified aminolipids.
29458475	6	22	contain	contained	882:890	arg2	phosphatidylglycerol					916:935	phosphatidylglycerol	916:935	phosphatidylglycerol	916:935	The polar lipid profile of strain LCB256T contained diphosphatidylglycerol, phosphatidylglycerol, one unidentified phospholipid and two unidentified aminolipids.
29458475	6	22	contain	contained	882:890	arg2	aminolipids					989:999	two unidentified aminolipids	972:999	two unidentified aminolipids	972:999	The polar lipid profile of strain LCB256T contained diphosphatidylglycerol, phosphatidylglycerol, one unidentified phospholipid and two unidentified aminolipids.
29458475	6	22	contain	contained	882:890	arg2	diphosphatidylglycerol					892:913	diphosphatidylglycerol	892:913	diphosphatidylglycerol	892:913	The polar lipid profile of strain LCB256T contained diphosphatidylglycerol, phosphatidylglycerol, one unidentified phospholipid and two unidentified aminolipids.
29458475	6	22	contain	contained	882:890	arg1	profile					856:862	The polar lipid profile	840:862	The polar lipid profile of strain LCB256T	840:880	The polar lipid profile of strain LCB256T contained diphosphatidylglycerol, phosphatidylglycerol, one unidentified phospholipid and two unidentified aminolipids.
29458475	4	23	theme	sequence	605:612	arg1	similarity					614:623	highest sequence similarity	597:623	highest sequence similarity	597:623	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain LCB256T was most closely related to the two genera of Ornithinibacillus and Oceanobacillus, showing highest sequence similarity to Oceanobacillus limi KCTC 13823T (97.8 %) and Ornithinibacillus bavariensis WSBC 24001T (97.2 %).
29458475	3	24	theme	10-15 	352:357	arg1	NaCl					338:341	3-17 % (w/v) NaCl	325:341	3-17 % (w/v) NaCl (optimum 10-15 %)	325:359	Cells of strain LCB256T were Gram-stain-positive, aerobic, rod-shaped and grew at 3-17 % (w/v) NaCl (optimum 10-15 %), 10-52 °C (optimum 25-30 °C) and pH 7.0-9.0 (optimum 8.0).
29458475	3	24	theme	10-15 	352:357	arg1	%					358:358	optimum 10-15 %	344:358	optimum 10-15 %	344:358	Cells of strain LCB256T were Gram-stain-positive, aerobic, rod-shaped and grew at 3-17 % (w/v) NaCl (optimum 10-15 %), 10-52 °C (optimum 25-30 °C) and pH 7.0-9.0 (optimum 8.0).
29458475	6	25	theme	LCB256T	874:880	arg1	profile					856:862	The polar lipid profile	840:862	The polar lipid profile of strain LCB256T	840:880	The polar lipid profile of strain LCB256T contained diphosphatidylglycerol, phosphatidylglycerol, one unidentified phospholipid and two unidentified aminolipids.
29458475	10	26	theme	sp	1405:1406	arg1	species					1337:1343	a novel species	1329:1343	a novel species of the genus Ornithinibacillus, Ornithinibacillussalinisoli sp	1329:1406	Based on this polyphasic taxonomic study, a novel species of the genus Ornithinibacillus, Ornithinibacillussalinisoli sp.
29458475	10	26	theme	sp	1405:1406	arg1	study					1322:1326	this polyphasic taxonomic study	1296:1326	this polyphasic taxonomic study	1296:1326	Based on this polyphasic taxonomic study, a novel species of the genus Ornithinibacillus, Ornithinibacillussalinisoli sp.
29458475	1	27	theme	saline-alkali	89:101	arg1	soil					103:106	a saline-alkali soil	87:106	a saline-alkali soil	87:106	nov., a moderately halophilic bacterium isolated from a saline-alkali soil.
29458475	4	28	theme	WSBC	703:706	arg1	24001T					708:713	Ornithinibacillus bavariensis WSBC 24001T	673:713	Ornithinibacillus bavariensis WSBC 24001T (97.2 %)	673:722	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain LCB256T was most closely related to the two genera of Ornithinibacillus and Oceanobacillus, showing highest sequence similarity to Oceanobacillus limi KCTC 13823T (97.8 %) and Ornithinibacillus bavariensis WSBC 24001T (97.2 %).
29458475	4	28	theme	WSBC	703:706	arg1	%					721:721	97.2 %	716:721	97.2 %	716:721	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain LCB256T was most closely related to the two genera of Ornithinibacillus and Oceanobacillus, showing highest sequence similarity to Oceanobacillus limi KCTC 13823T (97.8 %) and Ornithinibacillus bavariensis WSBC 24001T (97.2 %).
29458475	3	29	theme	optimum	372:378	arg1	25-30 °C					380:387	optimum 25-30 °C	372:387	optimum 25-30 °C	372:387	Cells of strain LCB256T were Gram-stain-positive, aerobic, rod-shaped and grew at 3-17 % (w/v) NaCl (optimum 10-15 %), 10-52 °C (optimum 25-30 °C) and pH 7.0-9.0 (optimum 8.0).
29458475	3	29	theme	optimum	372:378	arg1	10-52 °C					362:369	10-52 °C	362:369	10-52 °C (optimum 25-30 °C)	362:388	Cells of strain LCB256T were Gram-stain-positive, aerobic, rod-shaped and grew at 3-17 % (w/v) NaCl (optimum 10-15 %), 10-52 °C (optimum 25-30 °C) and pH 7.0-9.0 (optimum 8.0).
29458475	5	30	theme	amino	743:747	arg1	acid					749:752	peptidoglycan amino acid	729:752	The peptidoglycan amino acid type	725:757	The peptidoglycan amino acid type was found to be A4β and the major respiratory quinone was determined to be MK-7.
29458475	5	31	theme	peptidoglycan	729:741	arg1	acid					749:752	peptidoglycan amino acid	729:752	The peptidoglycan amino acid type	725:757	The peptidoglycan amino acid type was found to be A4β and the major respiratory quinone was determined to be MK-7.
29458475	7	32	theme	dominant	1006:1013	arg1	anteiso-C15 					1041:1052	anteiso-C15 	1041:1052	anteiso-C15 	1041:1052	The dominant cellular fatty acids were anteiso-C15 : 0 and iso-C15 : 0.
29458475	7	32	theme	dominant	1006:1013	arg1	acids					1030:1034	The dominant cellular fatty acids	1002:1034	The dominant cellular fatty acids	1002:1034	The dominant cellular fatty acids were anteiso-C15 : 0 and iso-C15 : 0.
29458475	10	33	theme	taxonomic	1312:1320	arg1	species					1337:1343	a novel species	1329:1343	a novel species of the genus Ornithinibacillus, Ornithinibacillussalinisoli sp	1329:1406	Based on this polyphasic taxonomic study, a novel species of the genus Ornithinibacillus, Ornithinibacillussalinisoli sp.
29458475	10	33	theme	taxonomic	1312:1320	arg1	study					1322:1326	this polyphasic taxonomic study	1296:1326	this polyphasic taxonomic study	1296:1326	Based on this polyphasic taxonomic study, a novel species of the genus Ornithinibacillus, Ornithinibacillussalinisoli sp.
29458475	4	34	theme	Ornithinibacillus	673:689	arg1	24001T					708:713	Ornithinibacillus bavariensis WSBC 24001T	673:713	Ornithinibacillus bavariensis WSBC 24001T (97.2 %)	673:722	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain LCB256T was most closely related to the two genera of Ornithinibacillus and Oceanobacillus, showing highest sequence similarity to Oceanobacillus limi KCTC 13823T (97.8 %) and Ornithinibacillus bavariensis WSBC 24001T (97.2 %).
29458475	4	34	theme	Ornithinibacillus	673:689	arg1	%					721:721	97.2 %	716:721	97.2 %	716:721	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain LCB256T was most closely related to the two genera of Ornithinibacillus and Oceanobacillus, showing highest sequence similarity to Oceanobacillus limi KCTC 13823T (97.8 %) and Ornithinibacillus bavariensis WSBC 24001T (97.2 %).
29458475	4	35	theme	Ornithinibacillus	551:567	arg1	genera					541:546	the two genera	533:546	the two genera of Ornithinibacillus and Oceanobacillus	533:586	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain LCB256T was most closely related to the two genera of Ornithinibacillus and Oceanobacillus, showing highest sequence similarity to Oceanobacillus limi KCTC 13823T (97.8 %) and Ornithinibacillus bavariensis WSBC 24001T (97.2 %).
29458475	5	36	theme	acid	749:752	arg1	A4β					775:777	A4β	775:777	A4β	775:777	The peptidoglycan amino acid type was found to be A4β and the major respiratory quinone was determined to be MK-7.
29458475	5	36	theme	acid	749:752	arg1	type					754:757	The peptidoglycan amino acid type	725:757	The peptidoglycan amino acid type	725:757	The peptidoglycan amino acid type was found to be A4β and the major respiratory quinone was determined to be MK-7.
29458475	7	37	theme	cellular	1015:1022	arg1	anteiso-C15 					1041:1052	anteiso-C15 	1041:1052	anteiso-C15 	1041:1052	The dominant cellular fatty acids were anteiso-C15 : 0 and iso-C15 : 0.
29458475	7	37	theme	cellular	1015:1022	arg1	acids					1030:1034	The dominant cellular fatty acids	1002:1034	The dominant cellular fatty acids	1002:1034	The dominant cellular fatty acids were anteiso-C15 : 0 and iso-C15 : 0.
29458475	9	38	theme	strain	1155:1160	arg1	LCB256T					1162:1168	strain LCB256T	1155:1168	strain LCB256T	1155:1168	DNA-DNA relatedness values between strain LCB256T and Ornithinibacillus halophilus KCTC 13822T and Oceanobacillus limi KCTC 13823T were 46.2 and 34.8 %, respectively.
29458475	2	39	attach	isolated	170:177	arg2	LCB256T					151:157	strain LCB256T	144:157	strain LCB256T	144:157	A taxonomic study was performed on strain LCB256T, which was isolated from a saline-alkali soil sample taken from northwestern China.
29458475	2	39	attach	isolated	170:177	arg1	sample					205:210	a saline-alkali soil sample	184:210	a saline-alkali soil sample taken from northwestern China	184:240	A taxonomic study was performed on strain LCB256T, which was isolated from a saline-alkali soil sample taken from northwestern China.
29458475	4	40	theme	strain	490:495	arg1	LCB256T					497:503	strain LCB256T	490:503	strain LCB256T	490:503	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain LCB256T was most closely related to the two genera of Ornithinibacillus and Oceanobacillus, showing highest sequence similarity to Oceanobacillus limi KCTC 13823T (97.8 %) and Ornithinibacillus bavariensis WSBC 24001T (97.2 %).
29458475	3	41	theme	strain	252:257	arg1	LCB256T					259:265	strain LCB256T	252:265	strain LCB256T	252:265	Cells of strain LCB256T were Gram-stain-positive, aerobic, rod-shaped and grew at 3-17 % (w/v) NaCl (optimum 10-15 %), 10-52 °C (optimum 25-30 °C) and pH 7.0-9.0 (optimum 8.0).
29458475	2	42	theme	northwestern	223:234	arg1	China					236:240	northwestern China	223:240	northwestern China	223:240	A taxonomic study was performed on strain LCB256T, which was isolated from a saline-alkali soil sample taken from northwestern China.
29458475	4	43	theme	highest	597:603	arg1	similarity					614:623	highest sequence similarity	597:623	highest sequence similarity	597:623	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain LCB256T was most closely related to the two genera of Ornithinibacillus and Oceanobacillus, showing highest sequence similarity to Oceanobacillus limi KCTC 13823T (97.8 %) and Ornithinibacillus bavariensis WSBC 24001T (97.2 %).
29458475	4	44	theme	Oceanobacillus	573:586	arg1	genera					541:546	the two genera	533:546	the two genera of Ornithinibacillus and Oceanobacillus	533:586	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain LCB256T was most closely related to the two genera of Ornithinibacillus and Oceanobacillus, showing highest sequence similarity to Oceanobacillus limi KCTC 13823T (97.8 %) and Ornithinibacillus bavariensis WSBC 24001T (97.2 %).
29458475	4	45	theme	rRNA	455:458	arg1	sequences					465:473	16S rRNA gene sequences	451:473	16S rRNA gene sequences	451:473	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain LCB256T was most closely related to the two genera of Ornithinibacillus and Oceanobacillus, showing highest sequence similarity to Oceanobacillus limi KCTC 13823T (97.8 %) and Ornithinibacillus bavariensis WSBC 24001T (97.2 %).
29458475	3	46	theme	LCB256T	259:265	arg1	Cells					243:247	Cells	243:247	Cells of strain LCB256T	243:265	Cells of strain LCB256T were Gram-stain-positive, aerobic, rod-shaped and grew at 3-17 % (w/v) NaCl (optimum 10-15 %), 10-52 °C (optimum 25-30 °C) and pH 7.0-9.0 (optimum 8.0).
29458475	8	47	theme	39.3 mol	1109:1116	arg1	%					1117:1117	39.3 mol%	1109:1117	39.3 mol%	1109:1117	The G+C content of genomic DNA was 39.3 mol%.
29458475	8	47	theme	39.3 mol	1109:1116	arg1	G+C content					1078:1088	The G+C content	1074:1088	The G+C content of genomic DNA	1074:1103	The G+C content of genomic DNA was 39.3 mol%.
29458475	4	48	theme	16S	451:453	arg1	sequences					465:473	16S rRNA gene sequences	451:473	16S rRNA gene sequences	451:473	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain LCB256T was most closely related to the two genera of Ornithinibacillus and Oceanobacillus, showing highest sequence similarity to Oceanobacillus limi KCTC 13823T (97.8 %) and Ornithinibacillus bavariensis WSBC 24001T (97.2 %).
29458475	6	49	theme	strain	867:872	arg1	LCB256T					874:880	strain LCB256T	867:880	strain LCB256T	867:880	The polar lipid profile of strain LCB256T contained diphosphatidylglycerol, phosphatidylglycerol, one unidentified phospholipid and two unidentified aminolipids.
29458475	4	50	dep	Ornithinibacillus	673:689	arg1	bavariensis					691:701	bavariensis	691:701	bavariensis	691:701	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain LCB256T was most closely related to the two genera of Ornithinibacillus and Oceanobacillus, showing highest sequence similarity to Oceanobacillus limi KCTC 13823T (97.8 %) and Ornithinibacillus bavariensis WSBC 24001T (97.2 %).
29458475	7	51	dep	anteiso-C15 	1041:1052	arg1	 0					1054:1055	 0	1054:1055	 0	1054:1055	The dominant cellular fatty acids were anteiso-C15 : 0 and iso-C15 : 0.
29458475	7	51	dep	anteiso-C15 	1041:1052	arg1	 0					1070:1071	 0	1070:1071	 0	1070:1071	The dominant cellular fatty acids were anteiso-C15 : 0 and iso-C15 : 0.
29458475	7	51	dep	anteiso-C15 	1041:1052	arg1	iso-C15 					1061:1068	iso-C15 	1061:1068	iso-C15 	1061:1068	The dominant cellular fatty acids were anteiso-C15 : 0 and iso-C15 : 0.
29458475	2	52	theme	soil	200:203	arg1	sample					205:210	a saline-alkali soil sample	184:210	a saline-alkali soil sample taken from northwestern China	184:240	A taxonomic study was performed on strain LCB256T, which was isolated from a saline-alkali soil sample taken from northwestern China.
29458475	9	53	theme	KCTC	1203:1206	arg1	13822T					1208:1213	Ornithinibacillus halophilus KCTC 13822T	1174:1213	Ornithinibacillus halophilus KCTC 13822T	1174:1213	DNA-DNA relatedness values between strain LCB256T and Ornithinibacillus halophilus KCTC 13822T and Oceanobacillus limi KCTC 13823T were 46.2 and 34.8 %, respectively.
29458475	9	54	theme	limi	1234:1237	arg1	13823T					1244:1249	Oceanobacillus limi KCTC 13823T	1219:1249	Oceanobacillus limi KCTC 13823T	1219:1249	DNA-DNA relatedness values between strain LCB256T and Ornithinibacillus halophilus KCTC 13822T and Oceanobacillus limi KCTC 13823T were 46.2 and 34.8 %, respectively.
29458475	4	55	theme	KCTC	648:651	arg1	13823T					653:658	Oceanobacillus limi KCTC 13823T	628:658	Oceanobacillus limi KCTC 13823T (97.8 %)	628:667	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain LCB256T was most closely related to the two genera of Ornithinibacillus and Oceanobacillus, showing highest sequence similarity to Oceanobacillus limi KCTC 13823T (97.8 %) and Ornithinibacillus bavariensis WSBC 24001T (97.2 %).
29458475	4	55	theme	KCTC	648:651	arg1	%					666:666	97.8 %	661:666	97.8 %	661:666	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain LCB256T was most closely related to the two genera of Ornithinibacillus and Oceanobacillus, showing highest sequence similarity to Oceanobacillus limi KCTC 13823T (97.8 %) and Ornithinibacillus bavariensis WSBC 24001T (97.2 %).
29458475	4	56	theme	gene	460:463	arg1	sequences					465:473	16S rRNA gene sequences	451:473	16S rRNA gene sequences	451:473	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain LCB256T was most closely related to the two genera of Ornithinibacillus and Oceanobacillus, showing highest sequence similarity to Oceanobacillus limi KCTC 13823T (97.8 %) and Ornithinibacillus bavariensis WSBC 24001T (97.2 %).
29458475	6	57	theme	lipid	850:854	arg1	profile					856:862	The polar lipid profile	840:862	The polar lipid profile of strain LCB256T	840:880	The polar lipid profile of strain LCB256T contained diphosphatidylglycerol, phosphatidylglycerol, one unidentified phospholipid and two unidentified aminolipids.
29458475	2	58	theme	saline-alkali	186:198	arg1	sample					205:210	a saline-alkali soil sample	184:210	a saline-alkali soil sample taken from northwestern China	184:240	A taxonomic study was performed on strain LCB256T, which was isolated from a saline-alkali soil sample taken from northwestern China.
29458475	9	59	dep	Ornithinibacillus	1174:1190	arg1	halophilus					1192:1201	halophilus	1192:1201	halophilus	1192:1201	DNA-DNA relatedness values between strain LCB256T and Ornithinibacillus halophilus KCTC 13822T and Oceanobacillus limi KCTC 13823T were 46.2 and 34.8 %, respectively.
29458475	12	60	theme	type	1431:1434	arg1	LCB256T					1446:1452	LCB256T	1446:1452	LCB256T (=CGMCC 1.15809T=KCTC 33862T)	1446:1482	The type strain is LCB256T (=CGMCC 1.15809T=KCTC 33862T).
29458475	12	60	theme	type	1431:1434	arg1	strain					1436:1441	The type strain	1427:1441	The type strain	1427:1441	The type strain is LCB256T (=CGMCC 1.15809T=KCTC 33862T).
29458475	0	61	dep	sp	29:30	arg1	salinisoli					18:27	Ornithinibacillus salinisoli	0:27	Ornithinibacillus salinisoli	0:27	Ornithinibacillus salinisoli sp.
29458475	10	62	theme	polyphasic	1301:1310	arg1	species					1337:1343	a novel species	1329:1343	a novel species of the genus Ornithinibacillus, Ornithinibacillussalinisoli sp	1329:1406	Based on this polyphasic taxonomic study, a novel species of the genus Ornithinibacillus, Ornithinibacillussalinisoli sp.
29458475	10	62	theme	polyphasic	1301:1310	arg1	study					1322:1326	this polyphasic taxonomic study	1296:1326	this polyphasic taxonomic study	1296:1326	Based on this polyphasic taxonomic study, a novel species of the genus Ornithinibacillus, Ornithinibacillussalinisoli sp.
29458475	6	63	theme	unidentified	942:953	arg1	phospholipid					955:966	one unidentified phospholipid	938:966	one unidentified phospholipid	938:966	The polar lipid profile of strain LCB256T contained diphosphatidylglycerol, phosphatidylglycerol, one unidentified phospholipid and two unidentified aminolipids.
29458475	5	64	theme	major	787:791	arg1	quinone					805:811	the major respiratory quinone	783:811	the major respiratory quinone	783:811	The peptidoglycan amino acid type was found to be A4β and the major respiratory quinone was determined to be MK-7.
29458475	5	64	theme	major	787:791	arg1	MK-7					834:837	MK-7	834:837	MK-7	834:837	The peptidoglycan amino acid type was found to be A4β and the major respiratory quinone was determined to be MK-7.
29458475	9	65	theme	KCTC	1239:1242	arg1	13823T					1244:1249	Oceanobacillus limi KCTC 13823T	1219:1249	Oceanobacillus limi KCTC 13823T	1219:1249	DNA-DNA relatedness values between strain LCB256T and Ornithinibacillus halophilus KCTC 13822T and Oceanobacillus limi KCTC 13823T were 46.2 and 34.8 %, respectively.
29458475	10	66	theme	novel	1331:1335	arg1	species					1337:1343	a novel species	1329:1343	a novel species of the genus Ornithinibacillus, Ornithinibacillussalinisoli sp	1329:1406	Based on this polyphasic taxonomic study, a novel species of the genus Ornithinibacillus, Ornithinibacillussalinisoli sp.
29458475	10	66	theme	novel	1331:1335	arg1	study					1322:1326	this polyphasic taxonomic study	1296:1326	this polyphasic taxonomic study	1296:1326	Based on this polyphasic taxonomic study, a novel species of the genus Ornithinibacillus, Ornithinibacillussalinisoli sp.
29458475	9	67	theme	Oceanobacillus	1219:1232	arg1	13823T					1244:1249	Oceanobacillus limi KCTC 13823T	1219:1249	Oceanobacillus limi KCTC 13823T	1219:1249	DNA-DNA relatedness values between strain LCB256T and Ornithinibacillus halophilus KCTC 13822T and Oceanobacillus limi KCTC 13823T were 46.2 and 34.8 %, respectively.
29458475	5	68	theme	respiratory	793:803	arg1	quinone					805:811	the major respiratory quinone	783:811	the major respiratory quinone	783:811	The peptidoglycan amino acid type was found to be A4β and the major respiratory quinone was determined to be MK-7.
29458475	5	68	theme	respiratory	793:803	arg1	MK-7					834:837	MK-7	834:837	MK-7	834:837	The peptidoglycan amino acid type was found to be A4β and the major respiratory quinone was determined to be MK-7.
29458475	6	69	theme	polar	844:848	arg1	profile					856:862	The polar lipid profile	840:862	The polar lipid profile of strain LCB256T	840:880	The polar lipid profile of strain LCB256T contained diphosphatidylglycerol, phosphatidylglycerol, one unidentified phospholipid and two unidentified aminolipids.
31669567	2	0	theme	flow	622:625	arg1	rate					627:630	low gingival crevicular fluid flow rate	592:630	low gingival crevicular fluid flow rate	592:630	Thus, the aim was to develop a nanodelivery system for local application of antimicrobials, with evaluation in vitro using a newly developed micro flow-through apparatus that simulates local in-vivo conditions in the periodontal pocket: small resting volume, and low gingival crevicular fluid flow rate.
31669567	8	1	dep	7	1671:1671	arg1	to					1668:1669	to	1668:1669	to	1668:1669	The antimicrobial concentrations released from the nanofiber mats determined using the developed apparatus were above the minimal inhibitory concentrations against the periodontal pathogens for up to 7 days, which is valuable information for prediction of the efficacy of the nanodelivery system.
31669567	9	2	theme	delivery	1965:1972	arg1	system					1974:1979	any delivery system	1961:1979	any delivery system for application at target sites that have similar local conditions	1961:2046	Although this apparatus was specifically designed for characterization of formulations associated with treatments for periodontal disease, its applicability is much wide, as for development of any delivery system for application at target sites that have similar local conditions.
31669567	8	3	theme	valuable	1688:1695	arg1	information					1697:1707	valuable information	1688:1707	valuable information for prediction of the efficacy of the nanodelivery system	1688:1765	The antimicrobial concentrations released from the nanofiber mats determined using the developed apparatus were above the minimal inhibitory concentrations against the periodontal pathogens for up to 7 days, which is valuable information for prediction of the efficacy of the nanodelivery system.
31669567	3	4	theme	chitosan/	702:710	arg1	layer					743:747	a chitosan/ poly(ethylene) oxide nanofiber layer	700:747	a chitosan/ poly(ethylene) oxide nanofiber layer with 30% ciprofloxacin	700:770	We successfully developed a double-layer nanofiber mat composed of a chitosan/ poly(ethylene) oxide nanofiber layer with 30% ciprofloxacin, and a poly(ε-caprolactone) nanofiber layer with 5% metronidazole.
31669567	8	5	theme	system	1760:1765	arg1	efficacy					1731:1738	the efficacy	1727:1738	the efficacy of the nanodelivery system	1727:1765	The antimicrobial concentrations released from the nanofiber mats determined using the developed apparatus were above the minimal inhibitory concentrations against the periodontal pathogens for up to 7 days, which is valuable information for prediction of the efficacy of the nanodelivery system.
31669567	2	6	theme	crevicular	605:614	arg1	rate					627:630	low gingival crevicular fluid flow rate	592:630	low gingival crevicular fluid flow rate	592:630	Thus, the aim was to develop a nanodelivery system for local application of antimicrobials, with evaluation in vitro using a newly developed micro flow-through apparatus that simulates local in-vivo conditions in the periodontal pocket: small resting volume, and low gingival crevicular fluid flow rate.
31669567	6	7	theme	metronidazole	1172:1184	arg1	release					1161:1167	sustained release	1151:1167	sustained release of metronidazole	1151:1184	In contrast, sustained release of metronidazole was due to slow penetration of dissolution medium through the hydrophobic poly(ε-caprolactone) nanofiber layer.
31669567	3	8	theme	nanofiber	733:741	arg1	layer					743:747	a chitosan/ poly(ethylene) oxide nanofiber layer	700:747	a chitosan/ poly(ethylene) oxide nanofiber layer with 30% ciprofloxacin	700:770	We successfully developed a double-layer nanofiber mat composed of a chitosan/ poly(ethylene) oxide nanofiber layer with 30% ciprofloxacin, and a poly(ε-caprolactone) nanofiber layer with 5% metronidazole.
31669567	1	9	theme	effective	289:297	arg1	approaches					299:308	more effective approaches	284:308	more effective approaches	284:308	Periodontal disease is a widespread chronic condition associated with degradation of periodontal tissues that requires more effective approaches for its treatment.
31669567	9	10	contain	have	2018:2021	arg2	conditions					2037:2046	similar local conditions	2023:2046	similar local conditions	2023:2046	Although this apparatus was specifically designed for characterization of formulations associated with treatments for periodontal disease, its applicability is much wide, as for development of any delivery system for application at target sites that have similar local conditions.
31669567	9	10	contain	have	2018:2021	arg1	sites					2007:2011	target sites	2000:2011	target sites that have similar local conditions	2000:2046	Although this apparatus was specifically designed for characterization of formulations associated with treatments for periodontal disease, its applicability is much wide, as for development of any delivery system for application at target sites that have similar local conditions.
31669567	6	11	theme	hydrophobic	1248:1258	arg1	layer					1291:1295	the hydrophobic poly(ε-caprolactone) nanofiber layer	1244:1295	the hydrophobic poly(ε-caprolactone) nanofiber layer	1244:1295	In contrast, sustained release of metronidazole was due to slow penetration of dissolution medium through the hydrophobic poly(ε-caprolactone) nanofiber layer.
31669567	7	12	theme	plate	1445:1449	arg1	assays					1463:1468	plate antibiogram assays	1445:1468	plate antibiogram assays	1445:1468	The double-layer nanofiber mat developed showed antibacterial activity against Escherichia coli and Aggregatibacter actinomycetemcomitans based on plate antibiogram assays.
31669567	3	13	theme	%	756:756	arg1	ciprofloxacin					758:770	30% ciprofloxacin	754:770	30% ciprofloxacin	754:770	We successfully developed a double-layer nanofiber mat composed of a chitosan/ poly(ethylene) oxide nanofiber layer with 30% ciprofloxacin, and a poly(ε-caprolactone) nanofiber layer with 5% metronidazole.
31669567	1	14	theme	widespread	190:199	arg1	condition					209:217	a widespread chronic condition	188:217	a widespread chronic condition associated with degradation of periodontal tissues that requires more effective approaches for its treatment	188:326	Periodontal disease is a widespread chronic condition associated with degradation of periodontal tissues that requires more effective approaches for its treatment.
31669567	1	14	theme	widespread	190:199	arg1	disease					177:183	Periodontal disease	165:183	Periodontal disease	165:183	Periodontal disease is a widespread chronic condition associated with degradation of periodontal tissues that requires more effective approaches for its treatment.
31669567	2	15	theme	resting	572:578	arg1	volume					580:585	small resting volume	566:585	small resting volume	566:585	Thus, the aim was to develop a nanodelivery system for local application of antimicrobials, with evaluation in vitro using a newly developed micro flow-through apparatus that simulates local in-vivo conditions in the periodontal pocket: small resting volume, and low gingival crevicular fluid flow rate.
31669567	4	16	theme	designed	853:860	arg1	composition					862:872	The precisely designed composition	839:872	The precisely designed composition	839:872	The precisely designed composition enabled sustained in-vitro release of the antimicrobials according to their specific drug release mechanisms.
31669567	6	17	theme	medium	1229:1234	arg1	penetration					1202:1212	slow penetration	1197:1212	slow penetration of dissolution medium through the hydrophobic poly(ε-caprolactone) nanofiber layer	1197:1295	In contrast, sustained release of metronidazole was due to slow penetration of dissolution medium through the hydrophobic poly(ε-caprolactone) nanofiber layer.
31669567	9	18	theme	local	2031:2035	arg1	conditions					2037:2046	similar local conditions	2023:2046	similar local conditions	2023:2046	Although this apparatus was specifically designed for characterization of formulations associated with treatments for periodontal disease, its applicability is much wide, as for development of any delivery system for application at target sites that have similar local conditions.
31669567	0	19	theme	new	131:133	arg1	apparatus					154:162	a new micro flow-through apparatus	129:162	a new micro flow-through apparatus	129:162	Sustained release of antimicrobials from double-layer nanofiber mats for local treatment of periodontal disease, evaluated using a new micro flow-through apparatus.
31669567	3	20	with	layer	810:814	arg1	metronidazole					824:836	5% metronidazole	821:836	5% metronidazole	821:836	We successfully developed a double-layer nanofiber mat composed of a chitosan/ poly(ethylene) oxide nanofiber layer with 30% ciprofloxacin, and a poly(ε-caprolactone) nanofiber layer with 5% metronidazole.
31669567	3	20	with	layer	810:814	arg1	ciprofloxacin					758:770	30% ciprofloxacin	754:770	30% ciprofloxacin	754:770	We successfully developed a double-layer nanofiber mat composed of a chitosan/ poly(ethylene) oxide nanofiber layer with 30% ciprofloxacin, and a poly(ε-caprolactone) nanofiber layer with 5% metronidazole.
31669567	2	21	theme	low	592:594	arg1	rate					627:630	low gingival crevicular fluid flow rate	592:630	low gingival crevicular fluid flow rate	592:630	Thus, the aim was to develop a nanodelivery system for local application of antimicrobials, with evaluation in vitro using a newly developed micro flow-through apparatus that simulates local in-vivo conditions in the periodontal pocket: small resting volume, and low gingival crevicular fluid flow rate.
31669567	0	22	theme	flow-through	141:152	arg1	apparatus					154:162	a new micro flow-through apparatus	129:162	a new micro flow-through apparatus	129:162	Sustained release of antimicrobials from double-layer nanofiber mats for local treatment of periodontal disease, evaluated using a new micro flow-through apparatus.
31669567	8	23	theme	nanofiber	1522:1530	arg1	mats					1532:1535	the nanofiber mats	1518:1535	the nanofiber mats determined using the developed apparatus	1518:1576	The antimicrobial concentrations released from the nanofiber mats determined using the developed apparatus were above the minimal inhibitory concentrations against the periodontal pathogens for up to 7 days, which is valuable information for prediction of the efficacy of the nanodelivery system.
31669567	4	24	theme	sustained	882:890	arg1	release					901:907	sustained in-vitro release	882:907	sustained in-vitro release of the antimicrobials according to their specific drug release mechanisms	882:981	The precisely designed composition enabled sustained in-vitro release of the antimicrobials according to their specific drug release mechanisms.
31669567	2	25	from	conditions	528:537	arg1	pocket					558:563	the periodontal pocket	542:563	the periodontal pocket	542:563	Thus, the aim was to develop a nanodelivery system for local application of antimicrobials, with evaluation in vitro using a newly developed micro flow-through apparatus that simulates local in-vivo conditions in the periodontal pocket: small resting volume, and low gingival crevicular fluid flow rate.
31669567	6	26	theme	slow	1197:1200	arg1	penetration					1202:1212	slow penetration	1197:1212	slow penetration of dissolution medium through the hydrophobic poly(ε-caprolactone) nanofiber layer	1197:1295	In contrast, sustained release of metronidazole was due to slow penetration of dissolution medium through the hydrophobic poly(ε-caprolactone) nanofiber layer.
31669567	2	27	theme	in-vivo	520:526	arg1	conditions					528:537	local in-vivo conditions	514:537	local in-vivo conditions in the periodontal pocket	514:563	Thus, the aim was to develop a nanodelivery system for local application of antimicrobials, with evaluation in vitro using a newly developed micro flow-through apparatus that simulates local in-vivo conditions in the periodontal pocket: small resting volume, and low gingival crevicular fluid flow rate.
31669567	7	28	theme	antibacterial	1346:1358	arg1	activity					1360:1367	antibacterial activity	1346:1367	antibacterial activity against Escherichia coli and Aggregatibacter actinomycetemcomitans	1346:1434	The double-layer nanofiber mat developed showed antibacterial activity against Escherichia coli and Aggregatibacter actinomycetemcomitans based on plate antibiogram assays.
31669567	9	29	theme	formulations	1842:1853	arg1	characterization					1822:1837	characterization	1822:1837	characterization of formulations associated with treatments for periodontal disease	1822:1904	Although this apparatus was specifically designed for characterization of formulations associated with treatments for periodontal disease, its applicability is much wide, as for development of any delivery system for application at target sites that have similar local conditions.
31669567	3	30	theme	5	821:821	arg1	%					822:822	%	822:822	%	822:822	We successfully developed a double-layer nanofiber mat composed of a chitosan/ poly(ethylene) oxide nanofiber layer with 30% ciprofloxacin, and a poly(ε-caprolactone) nanofiber layer with 5% metronidazole.
31669567	5	31	from	pH	1062:1063	arg1	step					1002:1005	The rate-limiting step	984:1005	The rate-limiting step of ciprofloxacin release	984:1030	The rate-limiting step of ciprofloxacin release was its own low solubility at pH 7.4, when there was excess of solid drug present in the delivery system.
31669567	5	31	from	pH	1062:1063	arg1	solubility					1048:1057	its own low solubility	1036:1057	its own low solubility	1036:1057	The rate-limiting step of ciprofloxacin release was its own low solubility at pH 7.4, when there was excess of solid drug present in the delivery system.
31669567	5	32	theme	delivery	1121:1128	arg1	system					1130:1135	the delivery system	1117:1135	the delivery system	1117:1135	The rate-limiting step of ciprofloxacin release was its own low solubility at pH 7.4, when there was excess of solid drug present in the delivery system.
31669567	1	33	theme	Periodontal	165:175	arg1	condition					209:217	a widespread chronic condition	188:217	a widespread chronic condition associated with degradation of periodontal tissues that requires more effective approaches for its treatment	188:326	Periodontal disease is a widespread chronic condition associated with degradation of periodontal tissues that requires more effective approaches for its treatment.
31669567	1	33	theme	Periodontal	165:175	arg1	disease					177:183	Periodontal disease	165:183	Periodontal disease	165:183	Periodontal disease is a widespread chronic condition associated with degradation of periodontal tissues that requires more effective approaches for its treatment.
31669567	4	34	theme	drug	959:962	arg1	mechanisms					972:981	their specific drug release mechanisms	944:981	their specific drug release mechanisms	944:981	The precisely designed composition enabled sustained in-vitro release of the antimicrobials according to their specific drug release mechanisms.
31669567	0	35	theme	periodontal	92:102	arg1	disease					104:110	periodontal disease	92:110	periodontal disease	92:110	Sustained release of antimicrobials from double-layer nanofiber mats for local treatment of periodontal disease, evaluated using a new micro flow-through apparatus.
31669567	2	36	theme	antimicrobials	405:418	arg1	application					390:400	local application	384:400	local application of antimicrobials	384:418	Thus, the aim was to develop a nanodelivery system for local application of antimicrobials, with evaluation in vitro using a newly developed micro flow-through apparatus that simulates local in-vivo conditions in the periodontal pocket: small resting volume, and low gingival crevicular fluid flow rate.
31669567	5	37	theme	drug	1101:1104	arg1	present					1106:1112	solid drug present	1095:1112	solid drug present	1095:1112	The rate-limiting step of ciprofloxacin release was its own low solubility at pH 7.4, when there was excess of solid drug present in the delivery system.
31669567	8	38	theme	minimal	1593:1599	arg1	concentrations					1612:1625	the minimal inhibitory concentrations	1589:1625	the minimal inhibitory concentrations against the periodontal pathogens	1589:1659	The antimicrobial concentrations released from the nanofiber mats determined using the developed apparatus were above the minimal inhibitory concentrations against the periodontal pathogens for up to 7 days, which is valuable information for prediction of the efficacy of the nanodelivery system.
31669567	0	39	theme	Sustained	0:8	arg1	release					10:16	Sustained release	0:16	Sustained release	0:16	Sustained release of antimicrobials from double-layer nanofiber mats for local treatment of periodontal disease, evaluated using a new micro flow-through apparatus.
31669567	7	40	theme	double-layer	1302:1313	arg1	mat					1325:1327	The double-layer nanofiber mat	1298:1327	The double-layer nanofiber mat developed	1298:1337	The double-layer nanofiber mat developed showed antibacterial activity against Escherichia coli and Aggregatibacter actinomycetemcomitans based on plate antibiogram assays.
31669567	3	41	theme	nanofiber	674:682	arg1	mat					684:686	a double-layer nanofiber mat	659:686	a double-layer nanofiber mat composed of a chitosan/ poly(ethylene) oxide nanofiber layer with 30% ciprofloxacin, and a poly(ε-caprolactone) nanofiber layer with 5% metronidazole	659:836	We successfully developed a double-layer nanofiber mat composed of a chitosan/ poly(ethylene) oxide nanofiber layer with 30% ciprofloxacin, and a poly(ε-caprolactone) nanofiber layer with 5% metronidazole.
31669567	6	42	from	contrast	1141:1148	arg1	due					1190:1192	due	1190:1192	due	1190:1192	In contrast, sustained release of metronidazole was due to slow penetration of dissolution medium through the hydrophobic poly(ε-caprolactone) nanofiber layer.
31669567	5	43	theme	own	1040:1042	arg1	step					1002:1005	The rate-limiting step	984:1005	The rate-limiting step of ciprofloxacin release	984:1030	The rate-limiting step of ciprofloxacin release was its own low solubility at pH 7.4, when there was excess of solid drug present in the delivery system.
31669567	5	43	theme	own	1040:1042	arg1	solubility					1048:1057	its own low solubility	1036:1057	its own low solubility	1036:1057	The rate-limiting step of ciprofloxacin release was its own low solubility at pH 7.4, when there was excess of solid drug present in the delivery system.
31669567	2	44	theme	micro	470:474	arg1	apparatus					489:497	a newly developed micro flow-through apparatus	452:497	a newly developed micro flow-through apparatus that simulates local in-vivo conditions in the periodontal pocket: small resting volume, and low gingival crevicular fluid flow rate	452:630	Thus, the aim was to develop a nanodelivery system for local application of antimicrobials, with evaluation in vitro using a newly developed micro flow-through apparatus that simulates local in-vivo conditions in the periodontal pocket: small resting volume, and low gingival crevicular fluid flow rate.
31669567	2	45	from	rate	627:630	arg1	pocket					558:563	the periodontal pocket	542:563	the periodontal pocket	542:563	Thus, the aim was to develop a nanodelivery system for local application of antimicrobials, with evaluation in vitro using a newly developed micro flow-through apparatus that simulates local in-vivo conditions in the periodontal pocket: small resting volume, and low gingival crevicular fluid flow rate.
31669567	0	46	theme	nanofiber	54:62	arg1	mats					64:67	double-layer nanofiber mats	41:67	double-layer nanofiber mats for local treatment of periodontal disease	41:110	Sustained release of antimicrobials from double-layer nanofiber mats for local treatment of periodontal disease, evaluated using a new micro flow-through apparatus.
31669567	8	47	attach	released	1504:1511	arg2	concentrations					1489:1502	The antimicrobial concentrations	1471:1502	The antimicrobial concentrations released from the nanofiber mats determined using the developed apparatus	1471:1576	The antimicrobial concentrations released from the nanofiber mats determined using the developed apparatus were above the minimal inhibitory concentrations against the periodontal pathogens for up to 7 days, which is valuable information for prediction of the efficacy of the nanodelivery system.
31669567	8	47	attach	released	1504:1511	arg1	mats					1532:1535	the nanofiber mats	1518:1535	the nanofiber mats determined using the developed apparatus	1518:1576	The antimicrobial concentrations released from the nanofiber mats determined using the developed apparatus were above the minimal inhibitory concentrations against the periodontal pathogens for up to 7 days, which is valuable information for prediction of the efficacy of the nanodelivery system.
31669567	5	48	theme	ciprofloxacin	1010:1022	arg1	release					1024:1030	ciprofloxacin release	1010:1030	ciprofloxacin release	1010:1030	The rate-limiting step of ciprofloxacin release was its own low solubility at pH 7.4, when there was excess of solid drug present in the delivery system.
31669567	8	49	theme	antimicrobial	1475:1487	arg1	concentrations					1489:1502	The antimicrobial concentrations	1471:1502	The antimicrobial concentrations released from the nanofiber mats determined using the developed apparatus	1471:1576	The antimicrobial concentrations released from the nanofiber mats determined using the developed apparatus were above the minimal inhibitory concentrations against the periodontal pathogens for up to 7 days, which is valuable information for prediction of the efficacy of the nanodelivery system.
31669567	3	50	with	layer	743:747	arg1	metronidazole					824:836	5% metronidazole	821:836	5% metronidazole	821:836	We successfully developed a double-layer nanofiber mat composed of a chitosan/ poly(ethylene) oxide nanofiber layer with 30% ciprofloxacin, and a poly(ε-caprolactone) nanofiber layer with 5% metronidazole.
31669567	3	50	with	layer	743:747	arg1	ciprofloxacin					758:770	30% ciprofloxacin	754:770	30% ciprofloxacin	754:770	We successfully developed a double-layer nanofiber mat composed of a chitosan/ poly(ethylene) oxide nanofiber layer with 30% ciprofloxacin, and a poly(ε-caprolactone) nanofiber layer with 5% metronidazole.
31669567	1	51	theme	periodontal	250:260	arg1	tissues					262:268	periodontal tissues	250:268	periodontal tissues	250:268	Periodontal disease is a widespread chronic condition associated with degradation of periodontal tissues that requires more effective approaches for its treatment.
31669567	3	52	theme	ethylene	717:724	arg1	layer					743:747	a chitosan/ poly(ethylene) oxide nanofiber layer	700:747	a chitosan/ poly(ethylene) oxide nanofiber layer with 30% ciprofloxacin	700:770	We successfully developed a double-layer nanofiber mat composed of a chitosan/ poly(ethylene) oxide nanofiber layer with 30% ciprofloxacin, and a poly(ε-caprolactone) nanofiber layer with 5% metronidazole.
31669567	6	53	theme	nanofiber	1281:1289	arg1	layer					1291:1295	the hydrophobic poly(ε-caprolactone) nanofiber layer	1244:1295	the hydrophobic poly(ε-caprolactone) nanofiber layer	1244:1295	In contrast, sustained release of metronidazole was due to slow penetration of dissolution medium through the hydrophobic poly(ε-caprolactone) nanofiber layer.
31669567	2	54	theme	nanodelivery	360:371	arg1	system					373:378	a nanodelivery system	358:378	a nanodelivery system for local application of antimicrobials	358:418	Thus, the aim was to develop a nanodelivery system for local application of antimicrobials, with evaluation in vitro using a newly developed micro flow-through apparatus that simulates local in-vivo conditions in the periodontal pocket: small resting volume, and low gingival crevicular fluid flow rate.
31669567	2	55	theme	fluid	616:620	arg1	rate					627:630	low gingival crevicular fluid flow rate	592:630	low gingival crevicular fluid flow rate	592:630	Thus, the aim was to develop a nanodelivery system for local application of antimicrobials, with evaluation in vitro using a newly developed micro flow-through apparatus that simulates local in-vivo conditions in the periodontal pocket: small resting volume, and low gingival crevicular fluid flow rate.
31669567	9	56	theme	system	1974:1979	arg1	development					1946:1956	development	1946:1956	development of any delivery system for application at target sites that have similar local conditions	1946:2046	Although this apparatus was specifically designed for characterization of formulations associated with treatments for periodontal disease, its applicability is much wide, as for development of any delivery system for application at target sites that have similar local conditions.
31669567	2	57	from	volume	580:585	arg1	pocket					558:563	the periodontal pocket	542:563	the periodontal pocket	542:563	Thus, the aim was to develop a nanodelivery system for local application of antimicrobials, with evaluation in vitro using a newly developed micro flow-through apparatus that simulates local in-vivo conditions in the periodontal pocket: small resting volume, and low gingival crevicular fluid flow rate.
31669567	3	58	theme	poly	712:715	arg1	layer					743:747	a chitosan/ poly(ethylene) oxide nanofiber layer	700:747	a chitosan/ poly(ethylene) oxide nanofiber layer with 30% ciprofloxacin	700:770	We successfully developed a double-layer nanofiber mat composed of a chitosan/ poly(ethylene) oxide nanofiber layer with 30% ciprofloxacin, and a poly(ε-caprolactone) nanofiber layer with 5% metronidazole.
31669567	6	59	theme	ε-caprolactone	1265:1278	arg1	layer					1291:1295	the hydrophobic poly(ε-caprolactone) nanofiber layer	1244:1295	the hydrophobic poly(ε-caprolactone) nanofiber layer	1244:1295	In contrast, sustained release of metronidazole was due to slow penetration of dissolution medium through the hydrophobic poly(ε-caprolactone) nanofiber layer.
31669567	8	60	theme	nanodelivery	1747:1758	arg1	system					1760:1765	the nanodelivery system	1743:1765	the nanodelivery system	1743:1765	The antimicrobial concentrations released from the nanofiber mats determined using the developed apparatus were above the minimal inhibitory concentrations against the periodontal pathogens for up to 7 days, which is valuable information for prediction of the efficacy of the nanodelivery system.
31669567	2	61	theme	gingival	596:603	arg1	rate					627:630	low gingival crevicular fluid flow rate	592:630	low gingival crevicular fluid flow rate	592:630	Thus, the aim was to develop a nanodelivery system for local application of antimicrobials, with evaluation in vitro using a newly developed micro flow-through apparatus that simulates local in-vivo conditions in the periodontal pocket: small resting volume, and low gingival crevicular fluid flow rate.
31669567	6	62	theme	poly	1260:1263	arg1	layer					1291:1295	the hydrophobic poly(ε-caprolactone) nanofiber layer	1244:1295	the hydrophobic poly(ε-caprolactone) nanofiber layer	1244:1295	In contrast, sustained release of metronidazole was due to slow penetration of dissolution medium through the hydrophobic poly(ε-caprolactone) nanofiber layer.
31669567	6	63	theme	sustained	1151:1159	arg1	release					1161:1167	sustained release	1151:1167	sustained release of metronidazole	1151:1184	In contrast, sustained release of metronidazole was due to slow penetration of dissolution medium through the hydrophobic poly(ε-caprolactone) nanofiber layer.
31669567	9	64	theme	target	2000:2005	arg1	sites					2007:2011	target sites	2000:2011	target sites that have similar local conditions	2000:2046	Although this apparatus was specifically designed for characterization of formulations associated with treatments for periodontal disease, its applicability is much wide, as for development of any delivery system for application at target sites that have similar local conditions.
31669567	3	65	theme	oxide	727:731	arg1	layer					743:747	a chitosan/ poly(ethylene) oxide nanofiber layer	700:747	a chitosan/ poly(ethylene) oxide nanofiber layer with 30% ciprofloxacin	700:770	We successfully developed a double-layer nanofiber mat composed of a chitosan/ poly(ethylene) oxide nanofiber layer with 30% ciprofloxacin, and a poly(ε-caprolactone) nanofiber layer with 5% metronidazole.
31669567	7	66	theme	antibiogram	1451:1461	arg1	assays					1463:1468	plate antibiogram assays	1445:1468	plate antibiogram assays	1445:1468	The double-layer nanofiber mat developed showed antibacterial activity against Escherichia coli and Aggregatibacter actinomycetemcomitans based on plate antibiogram assays.
31669567	1	67	theme	chronic	201:207	arg1	condition					209:217	a widespread chronic condition	188:217	a widespread chronic condition associated with degradation of periodontal tissues that requires more effective approaches for its treatment	188:326	Periodontal disease is a widespread chronic condition associated with degradation of periodontal tissues that requires more effective approaches for its treatment.
31669567	1	67	theme	chronic	201:207	arg1	disease					177:183	Periodontal disease	165:183	Periodontal disease	165:183	Periodontal disease is a widespread chronic condition associated with degradation of periodontal tissues that requires more effective approaches for its treatment.
31669567	4	68	theme	antimicrobials	916:929	arg1	release					901:907	sustained in-vitro release	882:907	sustained in-vitro release of the antimicrobials according to their specific drug release mechanisms	882:981	The precisely designed composition enabled sustained in-vitro release of the antimicrobials according to their specific drug release mechanisms.
31669567	9	69	theme	similar	2023:2029	arg1	conditions					2037:2046	similar local conditions	2023:2046	similar local conditions	2023:2046	Although this apparatus was specifically designed for characterization of formulations associated with treatments for periodontal disease, its applicability is much wide, as for development of any delivery system for application at target sites that have similar local conditions.
31669567	3	70	theme	30	754:755	arg1	%					756:756	%	756:756	%	756:756	We successfully developed a double-layer nanofiber mat composed of a chitosan/ poly(ethylene) oxide nanofiber layer with 30% ciprofloxacin, and a poly(ε-caprolactone) nanofiber layer with 5% metronidazole.
31669567	0	71	theme	micro	135:139	arg1	apparatus					154:162	a new micro flow-through apparatus	129:162	a new micro flow-through apparatus	129:162	Sustained release of antimicrobials from double-layer nanofiber mats for local treatment of periodontal disease, evaluated using a new micro flow-through apparatus.
31669567	6	72	theme	dissolution	1217:1227	arg1	medium					1229:1234	dissolution medium	1217:1234	dissolution medium	1217:1234	In contrast, sustained release of metronidazole was due to slow penetration of dissolution medium through the hydrophobic poly(ε-caprolactone) nanofiber layer.
31669567	2	73	theme	periodontal	546:556	arg1	pocket					558:563	the periodontal pocket	542:563	the periodontal pocket	542:563	Thus, the aim was to develop a nanodelivery system for local application of antimicrobials, with evaluation in vitro using a newly developed micro flow-through apparatus that simulates local in-vivo conditions in the periodontal pocket: small resting volume, and low gingival crevicular fluid flow rate.
31669567	4	74	theme	in-vitro	892:899	arg1	release					901:907	sustained in-vitro release	882:907	sustained in-vitro release of the antimicrobials according to their specific drug release mechanisms	882:981	The precisely designed composition enabled sustained in-vitro release of the antimicrobials according to their specific drug release mechanisms.
31669567	9	75	from	sites	2007:2011	arg1	application					1985:1995	application	1985:1995	application at target sites that have similar local conditions	1985:2046	Although this apparatus was specifically designed for characterization of formulations associated with treatments for periodontal disease, its applicability is much wide, as for development of any delivery system for application at target sites that have similar local conditions.
31669567	3	76	theme	nanofiber	800:808	arg1	layer					810:814	a poly(ε-caprolactone) nanofiber layer	777:814	a poly(ε-caprolactone) nanofiber layer with 5% metronidazole	777:836	We successfully developed a double-layer nanofiber mat composed of a chitosan/ poly(ethylene) oxide nanofiber layer with 30% ciprofloxacin, and a poly(ε-caprolactone) nanofiber layer with 5% metronidazole.
31669567	0	77	theme	local	73:77	arg1	treatment					79:87	local treatment	73:87	local treatment of periodontal disease	73:110	Sustained release of antimicrobials from double-layer nanofiber mats for local treatment of periodontal disease, evaluated using a new micro flow-through apparatus.
31669567	2	78	theme	local	514:518	arg1	conditions					528:537	local in-vivo conditions	514:537	local in-vivo conditions in the periodontal pocket	514:563	Thus, the aim was to develop a nanodelivery system for local application of antimicrobials, with evaluation in vitro using a newly developed micro flow-through apparatus that simulates local in-vivo conditions in the periodontal pocket: small resting volume, and low gingival crevicular fluid flow rate.
31669567	8	79	theme	developed	1558:1566	arg1	apparatus					1568:1576	the developed apparatus	1554:1576	the developed apparatus	1554:1576	The antimicrobial concentrations released from the nanofiber mats determined using the developed apparatus were above the minimal inhibitory concentrations against the periodontal pathogens for up to 7 days, which is valuable information for prediction of the efficacy of the nanodelivery system.
31669567	6	80	from	due	1190:1192	arg1	contrast					1141:1148	contrast	1141:1148	contrast	1141:1148	In contrast, sustained release of metronidazole was due to slow penetration of dissolution medium through the hydrophobic poly(ε-caprolactone) nanofiber layer.
31669567	2	81	theme	small	566:570	arg1	volume					580:585	small resting volume	566:585	small resting volume	566:585	Thus, the aim was to develop a nanodelivery system for local application of antimicrobials, with evaluation in vitro using a newly developed micro flow-through apparatus that simulates local in-vivo conditions in the periodontal pocket: small resting volume, and low gingival crevicular fluid flow rate.
31669567	0	82	from	release	10:16	arg1	mats					64:67	double-layer nanofiber mats	41:67	double-layer nanofiber mats for local treatment of periodontal disease	41:110	Sustained release of antimicrobials from double-layer nanofiber mats for local treatment of periodontal disease, evaluated using a new micro flow-through apparatus.
31669567	3	83	theme	%	822:822	arg1	metronidazole					824:836	5% metronidazole	821:836	5% metronidazole	821:836	We successfully developed a double-layer nanofiber mat composed of a chitosan/ poly(ethylene) oxide nanofiber layer with 30% ciprofloxacin, and a poly(ε-caprolactone) nanofiber layer with 5% metronidazole.
31669567	0	84	theme	disease	104:110	arg1	treatment					79:87	local treatment	73:87	local treatment of periodontal disease	73:110	Sustained release of antimicrobials from double-layer nanofiber mats for local treatment of periodontal disease, evaluated using a new micro flow-through apparatus.
31669567	4	85	theme	specific	950:957	arg1	mechanisms					972:981	their specific drug release mechanisms	944:981	their specific drug release mechanisms	944:981	The precisely designed composition enabled sustained in-vitro release of the antimicrobials according to their specific drug release mechanisms.
31669567	0	86	from	mats	64:67	arg1	antimicrobials					21:34	antimicrobials	21:34	antimicrobials from double-layer nanofiber mats for local treatment of periodontal disease	21:110	Sustained release of antimicrobials from double-layer nanofiber mats for local treatment of periodontal disease, evaluated using a new micro flow-through apparatus.
31669567	0	86	from	mats	64:67	arg1	release					10:16	Sustained release	0:16	Sustained release	0:16	Sustained release of antimicrobials from double-layer nanofiber mats for local treatment of periodontal disease, evaluated using a new micro flow-through apparatus.
31669567	5	87	theme	solid	1095:1099	arg1	present					1106:1112	solid drug present	1095:1112	solid drug present	1095:1112	The rate-limiting step of ciprofloxacin release was its own low solubility at pH 7.4, when there was excess of solid drug present in the delivery system.
31669567	5	88	theme	rate-limiting	988:1000	arg1	solubility					1048:1057	its own low solubility	1036:1057	its own low solubility	1036:1057	The rate-limiting step of ciprofloxacin release was its own low solubility at pH 7.4, when there was excess of solid drug present in the delivery system.
31669567	5	88	theme	rate-limiting	988:1000	arg1	step					1002:1005	The rate-limiting step	984:1005	The rate-limiting step of ciprofloxacin release	984:1030	The rate-limiting step of ciprofloxacin release was its own low solubility at pH 7.4, when there was excess of solid drug present in the delivery system.
31669567	4	89	theme	release	964:970	arg1	mechanisms					972:981	their specific drug release mechanisms	944:981	their specific drug release mechanisms	944:981	The precisely designed composition enabled sustained in-vitro release of the antimicrobials according to their specific drug release mechanisms.
31669567	8	90	theme	inhibitory	1601:1610	arg1	concentrations					1612:1625	the minimal inhibitory concentrations	1589:1625	the minimal inhibitory concentrations against the periodontal pathogens	1589:1659	The antimicrobial concentrations released from the nanofiber mats determined using the developed apparatus were above the minimal inhibitory concentrations against the periodontal pathogens for up to 7 days, which is valuable information for prediction of the efficacy of the nanodelivery system.
31669567	5	91	theme	present	1106:1112	arg1	excess					1085:1090	excess	1085:1090	excess of solid drug present	1085:1112	The rate-limiting step of ciprofloxacin release was its own low solubility at pH 7.4, when there was excess of solid drug present in the delivery system.
31669567	7	92	theme	nanofiber	1315:1323	arg1	mat					1325:1327	The double-layer nanofiber mat	1298:1327	The double-layer nanofiber mat developed	1298:1337	The double-layer nanofiber mat developed showed antibacterial activity against Escherichia coli and Aggregatibacter actinomycetemcomitans based on plate antibiogram assays.
31669567	2	93	theme	local	384:388	arg1	application					390:400	local application	384:400	local application of antimicrobials	384:418	Thus, the aim was to develop a nanodelivery system for local application of antimicrobials, with evaluation in vitro using a newly developed micro flow-through apparatus that simulates local in-vivo conditions in the periodontal pocket: small resting volume, and low gingival crevicular fluid flow rate.
31669567	0	94	theme	antimicrobials	21:34	arg1	release					10:16	Sustained release	0:16	Sustained release	0:16	Sustained release of antimicrobials from double-layer nanofiber mats for local treatment of periodontal disease, evaluated using a new micro flow-through apparatus.
31669567	8	95	theme	periodontal	1639:1649	arg1	pathogens					1651:1659	the periodontal pathogens	1635:1659	the periodontal pathogens	1635:1659	The antimicrobial concentrations released from the nanofiber mats determined using the developed apparatus were above the minimal inhibitory concentrations against the periodontal pathogens for up to 7 days, which is valuable information for prediction of the efficacy of the nanodelivery system.
31669567	3	96	theme	double-layer	661:672	arg1	mat					684:686	a double-layer nanofiber mat	659:686	a double-layer nanofiber mat composed of a chitosan/ poly(ethylene) oxide nanofiber layer with 30% ciprofloxacin, and a poly(ε-caprolactone) nanofiber layer with 5% metronidazole	659:836	We successfully developed a double-layer nanofiber mat composed of a chitosan/ poly(ethylene) oxide nanofiber layer with 30% ciprofloxacin, and a poly(ε-caprolactone) nanofiber layer with 5% metronidazole.
31669567	0	97	theme	double-layer	41:52	arg1	mats					64:67	double-layer nanofiber mats	41:67	double-layer nanofiber mats for local treatment of periodontal disease	41:110	Sustained release of antimicrobials from double-layer nanofiber mats for local treatment of periodontal disease, evaluated using a new micro flow-through apparatus.
31669567	2	98	theme	flow-through	476:487	arg1	apparatus					489:497	a newly developed micro flow-through apparatus	452:497	a newly developed micro flow-through apparatus that simulates local in-vivo conditions in the periodontal pocket: small resting volume, and low gingival crevicular fluid flow rate	452:630	Thus, the aim was to develop a nanodelivery system for local application of antimicrobials, with evaluation in vitro using a newly developed micro flow-through apparatus that simulates local in-vivo conditions in the periodontal pocket: small resting volume, and low gingival crevicular fluid flow rate.
31669567	9	99	theme	periodontal	1886:1896	arg1	disease					1898:1904	periodontal disease	1886:1904	periodontal disease	1886:1904	Although this apparatus was specifically designed for characterization of formulations associated with treatments for periodontal disease, its applicability is much wide, as for development of any delivery system for application at target sites that have similar local conditions.
31669567	5	100	theme	low	1044:1046	arg1	step					1002:1005	The rate-limiting step	984:1005	The rate-limiting step of ciprofloxacin release	984:1030	The rate-limiting step of ciprofloxacin release was its own low solubility at pH 7.4, when there was excess of solid drug present in the delivery system.
31669567	5	100	theme	low	1044:1046	arg1	solubility					1048:1057	its own low solubility	1036:1057	its own low solubility	1036:1057	The rate-limiting step of ciprofloxacin release was its own low solubility at pH 7.4, when there was excess of solid drug present in the delivery system.
31669567	2	101	theme	developed	460:468	arg1	apparatus					489:497	a newly developed micro flow-through apparatus	452:497	a newly developed micro flow-through apparatus that simulates local in-vivo conditions in the periodontal pocket: small resting volume, and low gingival crevicular fluid flow rate	452:630	Thus, the aim was to develop a nanodelivery system for local application of antimicrobials, with evaluation in vitro using a newly developed micro flow-through apparatus that simulates local in-vivo conditions in the periodontal pocket: small resting volume, and low gingival crevicular fluid flow rate.
31669567	8	102	theme	efficacy	1731:1738	arg1	prediction					1713:1722	prediction	1713:1722	prediction of the efficacy of the nanodelivery system	1713:1765	The antimicrobial concentrations released from the nanofiber mats determined using the developed apparatus were above the minimal inhibitory concentrations against the periodontal pathogens for up to 7 days, which is valuable information for prediction of the efficacy of the nanodelivery system.
31669567	5	103	theme	release	1024:1030	arg1	solubility					1048:1057	its own low solubility	1036:1057	its own low solubility	1036:1057	The rate-limiting step of ciprofloxacin release was its own low solubility at pH 7.4, when there was excess of solid drug present in the delivery system.
31669567	5	103	theme	release	1024:1030	arg1	step					1002:1005	The rate-limiting step	984:1005	The rate-limiting step of ciprofloxacin release	984:1030	The rate-limiting step of ciprofloxacin release was its own low solubility at pH 7.4, when there was excess of solid drug present in the delivery system.
31669567	1	104	theme	tissues	262:268	arg1	degradation					235:245	degradation	235:245	degradation of periodontal tissues	235:268	Periodontal disease is a widespread chronic condition associated with degradation of periodontal tissues that requires more effective approaches for its treatment.
30950781	2	0	from	sample	166:171	arg1	Province					219:226	Province	219:226	Province	219:226	A rod-shaped, endospore-forming, aerobic bacterium, designated FJAT-46582T, was isolated from a sediment sample of the coastal region in Xiapu County, Fujian Province in China.
30950781	2	0	from	sample	166:171	arg1	Fujian					212:217	Fujian	212:217	Fujian	212:217	A rod-shaped, endospore-forming, aerobic bacterium, designated FJAT-46582T, was isolated from a sediment sample of the coastal region in Xiapu County, Fujian Province in China.
30950781	2	1	dep	rod-shaped	63:72	arg1	aerobic					94:100	aerobic	94:100	aerobic	94:100	A rod-shaped, endospore-forming, aerobic bacterium, designated FJAT-46582T, was isolated from a sediment sample of the coastal region in Xiapu County, Fujian Province in China.
30950781	2	1	dep	rod-shaped	63:72	arg1	endospore-forming					75:91	endospore-forming	75:91	endospore-forming	75:91	A rod-shaped, endospore-forming, aerobic bacterium, designated FJAT-46582T, was isolated from a sediment sample of the coastal region in Xiapu County, Fujian Province in China.
30950781	10	2	dep	name	1408:1411	arg1	sp					1433:1434	Bacillus xiapuensis sp	1413:1434	the name Bacillus xiapuensis sp	1404:1434	The phenotypic characters and taxono-genomics study revealed that strain FJAT-46582T represents a novel Bacillus species, for which the name Bacillus xiapuensis sp.
30950781	7	3	theme	sequence	810:817	arg1	similarity					819:828	the highest sequence similarity	798:828	the highest sequence similarity to Bacillus thermotolerans SGZ-8T (97.6 %) and Bacillus ectoinformans (97.1 %)	798:907	Phylogenetic analysis based on 16S rRNA gene sequences affiliated strain FJAT-46582T with the genus Bacillus, and showed the highest sequence similarity to Bacillus thermotolerans SGZ-8T (97.6 %) and Bacillus ectoinformans (97.1 %).
30950781	7	4	theme	gene	717:720	arg1	sequences					722:730	16S rRNA gene sequences	708:730	16S rRNA gene sequences affiliated strain FJAT-46582T with the genus Bacillus	708:784	Phylogenetic analysis based on 16S rRNA gene sequences affiliated strain FJAT-46582T with the genus Bacillus, and showed the highest sequence similarity to Bacillus thermotolerans SGZ-8T (97.6 %) and Bacillus ectoinformans (97.1 %).
30950781	8	5	theme	DNA-DNA	956:962	arg1	hybridization					964:976	in silico DNA-DNA hybridization	946:976	in silico DNA-DNA hybridization	946:976	The average nucleotide identity and in silico DNA-DNA hybridization values between strain FJAT-46582T and the most closely related species were 72.3 and 22.9 %, respectively, which were much lower than the thresholds commonly used to define species (96 and 70 %, respectively) indicating that it belonged to a different taxon.
30950781	4	6	theme	isoprenoid	424:433	arg1	MK-7					447:450	MK-7	447:450	MK-7	447:450	The cell-wall peptidoglycan contained meso-diaminopimelic acid and the isoprenoid quinone was MK-7.
30950781	4	6	theme	isoprenoid	424:433	arg1	quinone					435:441	the isoprenoid quinone	420:441	the isoprenoid quinone	420:441	The cell-wall peptidoglycan contained meso-diaminopimelic acid and the isoprenoid quinone was MK-7.
30950781	12	7	theme	33155=CCTCC AB	1492:1505	arg1	FJAT-46582T					1474:1484	FJAT-46582T	1474:1484	FJAT-46582T (=JCM 33155=CCTCC AB 2017047T)	1474:1515	The type strain is FJAT-46582T (=JCM 33155=CCTCC AB 2017047T).
30950781	12	7	theme	33155=CCTCC AB	1492:1505	arg1	2017047T					1507:1514	=JCM 33155=CCTCC AB 2017047T	1487:1514	=JCM 33155=CCTCC AB 2017047T	1487:1514	The type strain is FJAT-46582T (=JCM 33155=CCTCC AB 2017047T).
30950781	3	8	theme	%	297:297	arg1	%					307:307	0 %	305:307	0 %	305:307	Growth was observed at 10-30 °C (optimum, 25 °C), in 0-7.0 % NaCl (0 %) and at pH 6.0-11.0 (pH 8.0), respectively.
30950781	3	8	theme	%	297:297	arg1	NaCl					299:302	0-7.0 % NaCl	291:302	0-7.0 % NaCl (0 %)	291:308	Growth was observed at 10-30 °C (optimum, 25 °C), in 0-7.0 % NaCl (0 %) and at pH 6.0-11.0 (pH 8.0), respectively.
30950781	1	9	theme	marine	44:49	arg1	sediment					51:58	marine sediment	44:58	marine sediment	44:58	nov., isolated from marine sediment.
30950781	7	10	theme	16S	708:710	arg1	sequences					722:730	16S rRNA gene sequences	708:730	16S rRNA gene sequences affiliated strain FJAT-46582T with the genus Bacillus	708:784	Phylogenetic analysis based on 16S rRNA gene sequences affiliated strain FJAT-46582T with the genus Bacillus, and showed the highest sequence similarity to Bacillus thermotolerans SGZ-8T (97.6 %) and Bacillus ectoinformans (97.1 %).
30950781	3	11	dep	10-30 °C	261:268	arg1	25 °C					280:284	25 °C	280:284	25 °C	280:284	Growth was observed at 10-30 °C (optimum, 25 °C), in 0-7.0 % NaCl (0 %) and at pH 6.0-11.0 (pH 8.0), respectively.
30950781	3	11	dep	10-30 °C	261:268	arg1	optimum					271:277	optimum	271:277	optimum	271:277	Growth was observed at 10-30 °C (optimum, 25 °C), in 0-7.0 % NaCl (0 %) and at pH 6.0-11.0 (pH 8.0), respectively.
30950781	2	12	from	region	188:193	arg1	Province					219:226	Province	219:226	Province	219:226	A rod-shaped, endospore-forming, aerobic bacterium, designated FJAT-46582T, was isolated from a sediment sample of the coastal region in Xiapu County, Fujian Province in China.
30950781	2	12	from	region	188:193	arg1	Fujian					212:217	Fujian	212:217	Fujian	212:217	A rod-shaped, endospore-forming, aerobic bacterium, designated FJAT-46582T, was isolated from a sediment sample of the coastal region in Xiapu County, Fujian Province in China.
30950781	4	13	theme	cell-wall	357:365	arg1	peptidoglycan					367:379	The cell-wall peptidoglycan	353:379	The cell-wall peptidoglycan	353:379	The cell-wall peptidoglycan contained meso-diaminopimelic acid and the isoprenoid quinone was MK-7.
30950781	7	14	dep	thermotolerans	842:855	arg1	SGZ-8T					857:862	SGZ-8T	857:862	Bacillus thermotolerans SGZ-8T (97.6 %)	833:871	Phylogenetic analysis based on 16S rRNA gene sequences affiliated strain FJAT-46582T with the genus Bacillus, and showed the highest sequence similarity to Bacillus thermotolerans SGZ-8T (97.6 %) and Bacillus ectoinformans (97.1 %).
30950781	3	15	theme	0-7.0 	291:296	arg1	%					297:297	%	297:297	%	297:297	Growth was observed at 10-30 °C (optimum, 25 °C), in 0-7.0 % NaCl (0 %) and at pH 6.0-11.0 (pH 8.0), respectively.
30950781	4	16	theme	meso-diaminopimelic	391:409	arg1	acid					411:414	meso-diaminopimelic acid	391:414	meso-diaminopimelic acid	391:414	The cell-wall peptidoglycan contained meso-diaminopimelic acid and the isoprenoid quinone was MK-7.
30950781	6	17	theme	polar	583:587	arg1	diphosphatidylglycerol					601:622	diphosphatidylglycerol	601:622	diphosphatidylglycerol	601:622	The main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidyl ethanolamine.
30950781	6	17	theme	polar	583:587	arg1	lipids					589:594	The main polar lipids	574:594	The main polar lipids	574:594	The main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidyl ethanolamine.
30950781	12	18	theme	type	1459:1462	arg1	FJAT-46582T					1474:1484	FJAT-46582T	1474:1484	FJAT-46582T (=JCM 33155=CCTCC AB 2017047T)	1474:1515	The type strain is FJAT-46582T (=JCM 33155=CCTCC AB 2017047T).
30950781	12	18	theme	type	1459:1462	arg1	strain					1464:1469	The type strain	1455:1469	The type strain	1455:1469	The type strain is FJAT-46582T (=JCM 33155=CCTCC AB 2017047T).
30950781	0	19	theme	xiapuensis	9:18	arg1	sp					20:21	Bacillus xiapuensis sp	0:21	Bacillus xiapuensis sp.	0:22	Bacillus xiapuensis sp.
30950781	6	20	theme	main	578:581	arg1	diphosphatidylglycerol					601:622	diphosphatidylglycerol	601:622	diphosphatidylglycerol	601:622	The main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidyl ethanolamine.
30950781	6	20	theme	main	578:581	arg1	lipids					589:594	The main polar lipids	574:594	The main polar lipids	574:594	The main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidyl ethanolamine.
30950781	7	21	theme	Phylogenetic	677:688	arg1	analysis					690:697	Phylogenetic analysis	677:697	Phylogenetic analysis	677:697	Phylogenetic analysis based on 16S rRNA gene sequences affiliated strain FJAT-46582T with the genus Bacillus, and showed the highest sequence similarity to Bacillus thermotolerans SGZ-8T (97.6 %) and Bacillus ectoinformans (97.1 %).
30950781	0	22	theme	Bacillus	0:7	arg1	sp					20:21	Bacillus xiapuensis sp	0:21	Bacillus xiapuensis sp.	0:22	Bacillus xiapuensis sp.
30950781	8	23	dep	in	946:947	arg1	silico					949:954	silico	949:954	silico	949:954	The average nucleotide identity and in silico DNA-DNA hybridization values between strain FJAT-46582T and the most closely related species were 72.3 and 22.9 %, respectively, which were much lower than the thresholds commonly used to define species (96 and 70 %, respectively) indicating that it belonged to a different taxon.
30950781	8	24	theme	hybridization	964:976	arg1	%					1068:1068	72.3 and 22.9 %	1054:1068	72.3 and 22.9 %	1054:1068	The average nucleotide identity and in silico DNA-DNA hybridization values between strain FJAT-46582T and the most closely related species were 72.3 and 22.9 %, respectively, which were much lower than the thresholds commonly used to define species (96 and 70 %, respectively) indicating that it belonged to a different taxon.
30950781	8	24	theme	hybridization	964:976	arg1	values					978:983	The average nucleotide identity and in silico DNA-DNA hybridization values	910:983	The average nucleotide identity and in silico DNA-DNA hybridization values between strain FJAT-46582T and the most closely related species	910:1047	The average nucleotide identity and in silico DNA-DNA hybridization values between strain FJAT-46582T and the most closely related species were 72.3 and 22.9 %, respectively, which were much lower than the thresholds commonly used to define species (96 and 70 %, respectively) indicating that it belonged to a different taxon.
30950781	2	25	from	Province	219:226	arg1	sample					166:171	a sediment sample	155:171	a sediment sample of the coastal region in Xiapu County, Fujian Province in China	155:235	A rod-shaped, endospore-forming, aerobic bacterium, designated FJAT-46582T, was isolated from a sediment sample of the coastal region in Xiapu County, Fujian Province in China.
30950781	8	26	theme	different	1220:1228	arg1	taxon					1230:1234	a different taxon	1218:1234	a different taxon	1218:1234	The average nucleotide identity and in silico DNA-DNA hybridization values between strain FJAT-46582T and the most closely related species were 72.3 and 22.9 %, respectively, which were much lower than the thresholds commonly used to define species (96 and 70 %, respectively) indicating that it belonged to a different taxon.
30950781	10	27	theme	phenotypic	1276:1285	arg1	characters					1287:1296	The phenotypic characters	1272:1296	The phenotypic characters	1272:1296	The phenotypic characters and taxono-genomics study revealed that strain FJAT-46582T represents a novel Bacillus species, for which the name Bacillus xiapuensis sp.
30950781	9	28	theme	DNA	1241:1243	arg1	G+C content					1245:1255	The DNA G+C content	1237:1255	The DNA G+C content	1237:1255	The DNA G+C content was 44.2 mol%.
30950781	9	28	theme	DNA	1241:1243	arg1	%					1269:1269	44.2 mol%	1261:1269	44.2 mol%	1261:1269	The DNA G+C content was 44.2 mol%.
30950781	10	29	theme	xiapuensis	1422:1431	arg1	sp					1433:1434	Bacillus xiapuensis sp	1413:1434	the name Bacillus xiapuensis sp	1404:1434	The phenotypic characters and taxono-genomics study revealed that strain FJAT-46582T represents a novel Bacillus species, for which the name Bacillus xiapuensis sp.
30950781	2	30	theme	sediment	157:164	arg1	sample					166:171	a sediment sample	155:171	a sediment sample of the coastal region in Xiapu County, Fujian Province in China	155:235	A rod-shaped, endospore-forming, aerobic bacterium, designated FJAT-46582T, was isolated from a sediment sample of the coastal region in Xiapu County, Fujian Province in China.
30950781	8	31	dep	species	1151:1157	arg1	%					1170:1170	96 and 70 %	1160:1170	96 and 70 %	1160:1170	The average nucleotide identity and in silico DNA-DNA hybridization values between strain FJAT-46582T and the most closely related species were 72.3 and 22.9 %, respectively, which were much lower than the thresholds commonly used to define species (96 and 70 %, respectively) indicating that it belonged to a different taxon.
30950781	8	32	theme	related	1033:1039	arg1	species					1041:1047	the most closely related species	1016:1047	the most closely related species	1016:1047	The average nucleotide identity and in silico DNA-DNA hybridization values between strain FJAT-46582T and the most closely related species were 72.3 and 22.9 %, respectively, which were much lower than the thresholds commonly used to define species (96 and 70 %, respectively) indicating that it belonged to a different taxon.
30950781	3	33	located	observed	249:256	arg1	10-30 °C					261:268	10-30 °C	261:268	10-30 °C (optimum, 25 °C)	261:285	Growth was observed at 10-30 °C (optimum, 25 °C), in 0-7.0 % NaCl (0 %) and at pH 6.0-11.0 (pH 8.0), respectively.
30950781	3	33	located	observed	249:256	arg2	Growth					238:243	Growth	238:243	Growth	238:243	Growth was observed at 10-30 °C (optimum, 25 °C), in 0-7.0 % NaCl (0 %) and at pH 6.0-11.0 (pH 8.0), respectively.
30950781	10	34	theme	Bacillus	1413:1420	arg1	sp					1433:1434	Bacillus xiapuensis sp	1413:1434	the name Bacillus xiapuensis sp	1404:1434	The phenotypic characters and taxono-genomics study revealed that strain FJAT-46582T represents a novel Bacillus species, for which the name Bacillus xiapuensis sp.
30950781	8	35	theme	strain	993:998	arg1	FJAT-46582T					1000:1010	strain FJAT-46582T	993:1010	strain FJAT-46582T	993:1010	The average nucleotide identity and in silico DNA-DNA hybridization values between strain FJAT-46582T and the most closely related species were 72.3 and 22.9 %, respectively, which were much lower than the thresholds commonly used to define species (96 and 70 %, respectively) indicating that it belonged to a different taxon.
30950781	10	36	theme	Bacillus	1376:1383	arg1	species					1385:1391	a novel Bacillus species	1368:1391	a novel Bacillus species	1368:1391	The phenotypic characters and taxono-genomics study revealed that strain FJAT-46582T represents a novel Bacillus species, for which the name Bacillus xiapuensis sp.
30950781	8	37	theme	nucleotide	922:931	arg1	identity					933:940	average nucleotide identity	914:940	average nucleotide identity	914:940	The average nucleotide identity and in silico DNA-DNA hybridization values between strain FJAT-46582T and the most closely related species were 72.3 and 22.9 %, respectively, which were much lower than the thresholds commonly used to define species (96 and 70 %, respectively) indicating that it belonged to a different taxon.
30950781	10	38	theme	strain	1338:1343	arg1	FJAT-46582T					1345:1355	strain FJAT-46582T	1338:1355	strain FJAT-46582T	1338:1355	The phenotypic characters and taxono-genomics study revealed that strain FJAT-46582T represents a novel Bacillus species, for which the name Bacillus xiapuensis sp.
30950781	5	39	theme	main	457:460	arg1	anteiso-C17 					479:490	anteiso-C17 	479:490	anteiso-C17 	479:490	The main fatty acids were anteiso-C17 : 0 (26.5 %), anteiso-C15 : 0 (19.6 %), iso-C15 : 0 (14.4 %) and C16 : 0 (10.5 %).
30950781	5	39	theme	main	457:460	arg1	acids					468:472	The main fatty acids	453:472	The main fatty acids	453:472	The main fatty acids were anteiso-C17 : 0 (26.5 %), anteiso-C15 : 0 (19.6 %), iso-C15 : 0 (14.4 %) and C16 : 0 (10.5 %).
30950781	4	40	contain	contained	381:389	arg1	peptidoglycan					367:379	The cell-wall peptidoglycan	353:379	The cell-wall peptidoglycan	353:379	The cell-wall peptidoglycan contained meso-diaminopimelic acid and the isoprenoid quinone was MK-7.
30950781	4	40	contain	contained	381:389	arg2	acid					411:414	meso-diaminopimelic acid	391:414	meso-diaminopimelic acid	391:414	The cell-wall peptidoglycan contained meso-diaminopimelic acid and the isoprenoid quinone was MK-7.
30950781	8	41	theme	average	914:920	arg1	identity					933:940	average nucleotide identity	914:940	average nucleotide identity	914:940	The average nucleotide identity and in silico DNA-DNA hybridization values between strain FJAT-46582T and the most closely related species were 72.3 and 22.9 %, respectively, which were much lower than the thresholds commonly used to define species (96 and 70 %, respectively) indicating that it belonged to a different taxon.
30950781	2	42	theme	region	188:193	arg1	sample					166:171	a sediment sample	155:171	a sediment sample of the coastal region in Xiapu County, Fujian Province in China	155:235	A rod-shaped, endospore-forming, aerobic bacterium, designated FJAT-46582T, was isolated from a sediment sample of the coastal region in Xiapu County, Fujian Province in China.
30950781	6	43	theme	phosphatidyl	650:661	arg1	ethanolamine					663:674	phosphatidyl ethanolamine	650:674	phosphatidyl ethanolamine	650:674	The main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidyl ethanolamine.
30950781	5	44	theme	fatty	462:466	arg1	anteiso-C17 					479:490	anteiso-C17 	479:490	anteiso-C17 	479:490	The main fatty acids were anteiso-C17 : 0 (26.5 %), anteiso-C15 : 0 (19.6 %), iso-C15 : 0 (14.4 %) and C16 : 0 (10.5 %).
30950781	5	44	theme	fatty	462:466	arg1	acids					468:472	The main fatty acids	453:472	The main fatty acids	453:472	The main fatty acids were anteiso-C17 : 0 (26.5 %), anteiso-C15 : 0 (19.6 %), iso-C15 : 0 (14.4 %) and C16 : 0 (10.5 %).
30950781	2	45	attach	isolated	141:148	arg1	sample					166:171	a sediment sample	155:171	a sediment sample of the coastal region in Xiapu County, Fujian Province in China	155:235	A rod-shaped, endospore-forming, aerobic bacterium, designated FJAT-46582T, was isolated from a sediment sample of the coastal region in Xiapu County, Fujian Province in China.
30950781	2	45	attach	isolated	141:148	arg2	bacterium					102:110	A rod-shaped, endospore-forming, aerobic bacterium	61:110	A rod-shaped, endospore-forming, aerobic bacterium	61:110	A rod-shaped, endospore-forming, aerobic bacterium, designated FJAT-46582T, was isolated from a sediment sample of the coastal region in Xiapu County, Fujian Province in China.
30950781	7	46	theme	strain	743:748	arg1	FJAT-46582T					750:760	strain FJAT-46582T	743:760	strain FJAT-46582T	743:760	Phylogenetic analysis based on 16S rRNA gene sequences affiliated strain FJAT-46582T with the genus Bacillus, and showed the highest sequence similarity to Bacillus thermotolerans SGZ-8T (97.6 %) and Bacillus ectoinformans (97.1 %).
30950781	2	47	theme	rod-shaped	63:72	arg1	bacterium					102:110	A rod-shaped, endospore-forming, aerobic bacterium	61:110	A rod-shaped, endospore-forming, aerobic bacterium	61:110	A rod-shaped, endospore-forming, aerobic bacterium, designated FJAT-46582T, was isolated from a sediment sample of the coastal region in Xiapu County, Fujian Province in China.
30950781	2	48	theme	coastal	180:186	arg1	region					188:193	the coastal region	176:193	the coastal region in Xiapu County, Fujian Province in China	176:235	A rod-shaped, endospore-forming, aerobic bacterium, designated FJAT-46582T, was isolated from a sediment sample of the coastal region in Xiapu County, Fujian Province in China.
30950781	12	49	theme	=JCM	1487:1490	arg1	FJAT-46582T					1474:1484	FJAT-46582T	1474:1484	FJAT-46582T (=JCM 33155=CCTCC AB 2017047T)	1474:1515	The type strain is FJAT-46582T (=JCM 33155=CCTCC AB 2017047T).
30950781	12	49	theme	=JCM	1487:1490	arg1	2017047T					1507:1514	=JCM 33155=CCTCC AB 2017047T	1487:1514	=JCM 33155=CCTCC AB 2017047T	1487:1514	The type strain is FJAT-46582T (=JCM 33155=CCTCC AB 2017047T).
30950781	8	50	theme	identity	933:940	arg1	%					1068:1068	72.3 and 22.9 %	1054:1068	72.3 and 22.9 %	1054:1068	The average nucleotide identity and in silico DNA-DNA hybridization values between strain FJAT-46582T and the most closely related species were 72.3 and 22.9 %, respectively, which were much lower than the thresholds commonly used to define species (96 and 70 %, respectively) indicating that it belonged to a different taxon.
30950781	8	50	theme	identity	933:940	arg1	values					978:983	The average nucleotide identity and in silico DNA-DNA hybridization values	910:983	The average nucleotide identity and in silico DNA-DNA hybridization values between strain FJAT-46582T and the most closely related species	910:1047	The average nucleotide identity and in silico DNA-DNA hybridization values between strain FJAT-46582T and the most closely related species were 72.3 and 22.9 %, respectively, which were much lower than the thresholds commonly used to define species (96 and 70 %, respectively) indicating that it belonged to a different taxon.
30950781	9	51	theme	44.2 mol	1261:1268	arg1	G+C content					1245:1255	The DNA G+C content	1237:1255	The DNA G+C content	1237:1255	The DNA G+C content was 44.2 mol%.
30950781	9	51	theme	44.2 mol	1261:1268	arg1	%					1269:1269	44.2 mol%	1261:1269	44.2 mol%	1261:1269	The DNA G+C content was 44.2 mol%.
30950781	10	52	theme	taxono-genomics	1302:1316	arg1	study					1318:1322	taxono-genomics study	1302:1322	taxono-genomics study	1302:1322	The phenotypic characters and taxono-genomics study revealed that strain FJAT-46582T represents a novel Bacillus species, for which the name Bacillus xiapuensis sp.
30950781	8	53	theme	in	946:947	arg1	hybridization					964:976	in silico DNA-DNA hybridization	946:976	in silico DNA-DNA hybridization	946:976	The average nucleotide identity and in silico DNA-DNA hybridization values between strain FJAT-46582T and the most closely related species were 72.3 and 22.9 %, respectively, which were much lower than the thresholds commonly used to define species (96 and 70 %, respectively) indicating that it belonged to a different taxon.
30950781	7	54	theme	highest	802:808	arg1	similarity					819:828	the highest sequence similarity	798:828	the highest sequence similarity to Bacillus thermotolerans SGZ-8T (97.6 %) and Bacillus ectoinformans (97.1 %)	798:907	Phylogenetic analysis based on 16S rRNA gene sequences affiliated strain FJAT-46582T with the genus Bacillus, and showed the highest sequence similarity to Bacillus thermotolerans SGZ-8T (97.6 %) and Bacillus ectoinformans (97.1 %).
30950781	7	55	theme	rRNA	712:715	arg1	sequences					722:730	16S rRNA gene sequences	708:730	16S rRNA gene sequences affiliated strain FJAT-46582T with the genus Bacillus	708:784	Phylogenetic analysis based on 16S rRNA gene sequences affiliated strain FJAT-46582T with the genus Bacillus, and showed the highest sequence similarity to Bacillus thermotolerans SGZ-8T (97.6 %) and Bacillus ectoinformans (97.1 %).
30950781	10	56	theme	novel	1370:1374	arg1	species					1385:1391	a novel Bacillus species	1368:1391	a novel Bacillus species	1368:1391	The phenotypic characters and taxono-genomics study revealed that strain FJAT-46582T represents a novel Bacillus species, for which the name Bacillus xiapuensis sp.
31274399	10	0	from	Neomicrococcus	1466:1479	arg1	strains					1428:1434	the strains	1424:1434	the strains from the genera Arthrobacter, Neomicrococcus,Citricoccus and Glutamicibacter	1424:1511	The fatty acid and menaquinone profile differentiated the strains from the genera Arthrobacter, Neomicrococcus,Citricoccus and Glutamicibacter.
31274399	5	1	theme	16S	592:594	arg1	similarity					615:624	The 16S rRNA gene sequence similarity	588:624	The 16S rRNA gene sequence similarity between the isolates and the next related type strains	588:679	The 16S rRNA gene sequence similarity between the isolates and the next related type strains was below 97.3 %.
31274399	10	2	from	Citricoccus	1481:1491	arg1	strains					1428:1434	the strains	1424:1434	the strains from the genera Arthrobacter, Neomicrococcus,Citricoccus and Glutamicibacter	1424:1511	The fatty acid and menaquinone profile differentiated the strains from the genera Arthrobacter, Neomicrococcus,Citricoccus and Glutamicibacter.
31274399	11	3	theme	analyses	1573:1580	arg1	results					1518:1524	The results	1514:1524	The results of phylogenetic, phenotypic and chemotaxonomic analyses	1514:1580	The results of phylogenetic, phenotypic and chemotaxonomic analyses indicated that the isolates belonged to two novel species of a novel genus, for which the names Galactobacter caseinivorans gen. nov., sp.
31274399	5	4	theme	gene	601:604	arg1	similarity					615:624	The 16S rRNA gene sequence similarity	588:624	The 16S rRNA gene sequence similarity between the isolates and the next related type strains	588:679	The 16S rRNA gene sequence similarity between the isolates and the next related type strains was below 97.3 %.
31274399	10	5	from	Glutamicibacter	1497:1511	arg1	strains					1428:1434	the strains	1424:1434	the strains from the genera Arthrobacter, Neomicrococcus,Citricoccus and Glutamicibacter	1424:1511	The fatty acid and menaquinone profile differentiated the strains from the genera Arthrobacter, Neomicrococcus,Citricoccus and Glutamicibacter.
31274399	3	6	theme	bacterial	225:233	arg1	Four					188:191	Four	188:191	Four	188:191	Four Gram-stain positive, rod-shaped bacterial isolates, strains JZ R-183T, JZ RK-117, DI-46 and JZ R-35T, were recovered from bulk tank raw cow's milk from three different dairy farms in Germany.
31274399	3	6	theme	bacterial	225:233	arg1	isolates					235:242	rod-shaped bacterial isolates	214:242	rod-shaped bacterial isolates	214:242	Four Gram-stain positive, rod-shaped bacterial isolates, strains JZ R-183T, JZ RK-117, DI-46 and JZ R-35T, were recovered from bulk tank raw cow's milk from three different dairy farms in Germany.
31274399	12	7	dep	sp	1757:1758	arg1	valiniphilus					1744:1755	nov. and Galactobacter valiniphilus	1721:1755	nov. and Galactobacter valiniphilus	1721:1755	nov. and Galactobacter valiniphilus sp.
31274399	2	8	theme	Micrococcaceae	121:134	arg1	nov.					83:86	nov.	83:86	nov.	83:86	nov., two novel species of the family Micrococcaceae, isolated from high bacterial count raw cow's milk.
31274399	2	8	theme	Micrococcaceae	121:134	arg1	species					99:105	two novel species	89:105	two novel species of the family Micrococcaceae	89:134	nov., two novel species of the family Micrococcaceae, isolated from high bacterial count raw cow's milk.
31274399	6	9	theme	16S	724:726	arg1	rRNA					728:731	16S rRNA	724:731	16S rRNA	724:731	Phylogenetic analysis of 16S rRNA, recA and gyrB genes revealed that these isolates formed two different groups in an independent cluster within the family Micrococcaceae.
31274399	14	10	theme	JZ	1801:1802	arg1	30902T					1829:1834	=DSM 107700T=LMG 30902T	1812:1834	=DSM 107700T=LMG 30902T	1812:1834	The type strains are JZ R-183T (=DSM 107700T=LMG 30902T) and JZ R-35T (=DSM 107699T=LMG 30901T).
31274399	14	10	theme	JZ	1801:1802	arg1	strains					1789:1795	The type strains	1780:1795	The type strains	1780:1795	The type strains are JZ R-183T (=DSM 107700T=LMG 30902T) and JZ R-35T (=DSM 107699T=LMG 30901T).
31274399	14	10	theme	JZ	1801:1802	arg1	R-183T					1804:1809	JZ R-183T	1801:1809	JZ R-183T (=DSM 107700T=LMG 30902T)	1801:1835	The type strains are JZ R-183T (=DSM 107700T=LMG 30902T) and JZ R-35T (=DSM 107699T=LMG 30901T).
31274399	6	11	theme	different	794:802	arg1	groups					804:809	two different groups	790:809	two different groups	790:809	Phylogenetic analysis of 16S rRNA, recA and gyrB genes revealed that these isolates formed two different groups in an independent cluster within the family Micrococcaceae.
31274399	11	12	theme	caseinivorans	1692:1704	arg1	sp					1717:1718	sp	1717:1718	sp	1717:1718	The results of phylogenetic, phenotypic and chemotaxonomic analyses indicated that the isolates belonged to two novel species of a novel genus, for which the names Galactobacter caseinivorans gen. nov., sp.
31274399	11	12	theme	caseinivorans	1692:1704	arg1	nov.					1711:1714	Galactobacter caseinivorans gen. nov.	1678:1714	Galactobacter caseinivorans gen. nov.	1678:1714	The results of phylogenetic, phenotypic and chemotaxonomic analyses indicated that the isolates belonged to two novel species of a novel genus, for which the names Galactobacter caseinivorans gen. nov., sp.
31274399	3	13	theme	JZ	253:254	arg1	R-183T					256:261	JZ R-183T	253:261	JZ R-183T	253:261	Four Gram-stain positive, rod-shaped bacterial isolates, strains JZ R-183T, JZ RK-117, DI-46 and JZ R-35T, were recovered from bulk tank raw cow's milk from three different dairy farms in Germany.
31274399	3	13	theme	JZ	253:254	arg1	strains					245:251	strains JZ R-183T, JZ RK-117, DI-46 and JZ R-35T	245:292	strains JZ R-183T, JZ RK-117, DI-46 and JZ R-35T	245:292	Four Gram-stain positive, rod-shaped bacterial isolates, strains JZ R-183T, JZ RK-117, DI-46 and JZ R-35T, were recovered from bulk tank raw cow's milk from three different dairy farms in Germany.
31274399	8	14	theme	polar	1114:1118	arg1	pattern					1126:1132	the polar lipid pattern	1110:1132	the polar lipid pattern	1110:1132	The menaquinones MK-9(H2) and MK-7(H2) were present in all of the isolates and the polar lipid pattern contained the phospholipids diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol and a glycolipid.
31274399	4	15	dep	genera	519:524	arg1	Citricoccus					575:585	Citricoccus	575:585	Citricoccus	575:585	Analysis of their 16S rRNA gene sequences indicated that these isolates belonged to the family Micrococcaceae, closely related to the genera Arthrobacter, Neomicrococcus,Glutamicibacter and Citricoccus.
31274399	4	15	dep	genera	519:524	arg1	Glutamicibacter					555:569	Glutamicibacter	555:569	Glutamicibacter	555:569	Analysis of their 16S rRNA gene sequences indicated that these isolates belonged to the family Micrococcaceae, closely related to the genera Arthrobacter, Neomicrococcus,Glutamicibacter and Citricoccus.
31274399	4	15	dep	genera	519:524	arg1	Neomicrococcus					540:553	Neomicrococcus	540:553	Neomicrococcus	540:553	Analysis of their 16S rRNA gene sequences indicated that these isolates belonged to the family Micrococcaceae, closely related to the genera Arthrobacter, Neomicrococcus,Glutamicibacter and Citricoccus.
31274399	9	16	theme	cell	1347:1350	arg1	wall					1352:1355	dominating cell wall	1336:1355	dominating cell wall amino acids	1336:1367	The peptidoglycan type of the isolates was A4α, with alanine, lysine and glutamate as dominating cell wall amino acids.
31274399	9	17	theme	amino	1357:1361	arg1	acids					1363:1367	dominating cell wall amino acids	1336:1367	dominating cell wall amino acids	1336:1367	The peptidoglycan type of the isolates was A4α, with alanine, lysine and glutamate as dominating cell wall amino acids.
31274399	11	18	theme	phylogenetic	1529:1540	arg1	analyses					1573:1580	phylogenetic, phenotypic and chemotaxonomic analyses	1529:1580	phylogenetic, phenotypic and chemotaxonomic analyses	1529:1580	The results of phylogenetic, phenotypic and chemotaxonomic analyses indicated that the isolates belonged to two novel species of a novel genus, for which the names Galactobacter caseinivorans gen. nov., sp.
31274399	7	19	theme	iso-C15 	975:982	arg1	iso-C15 					975:982	iso-C15 	975:982	iso-C15 	975:982	Chemotaxonomic analyses determined anteiso-C15 : 0 as predominant fatty acid, but also large amounts of iso-C15 : 0, iso-C16 : 0 and iso-C17 : 0 were detected.
31274399	7	19	theme	iso-C15 	975:982	arg1	amounts					964:970	large amounts	958:970	large amounts of iso-C15 	958:982	Chemotaxonomic analyses determined anteiso-C15 : 0 as predominant fatty acid, but also large amounts of iso-C15 : 0, iso-C16 : 0 and iso-C17 : 0 were detected.
31274399	7	19	theme	iso-C15 	975:982	arg1	acid					943:946	predominant fatty acid	925:946	predominant fatty acid	925:946	Chemotaxonomic analyses determined anteiso-C15 : 0 as predominant fatty acid, but also large amounts of iso-C15 : 0, iso-C16 : 0 and iso-C17 : 0 were detected.
31274399	6	20	theme	family	848:853	arg1	Micrococcaceae					855:868	the family Micrococcaceae	844:868	the family Micrococcaceae	844:868	Phylogenetic analysis of 16S rRNA, recA and gyrB genes revealed that these isolates formed two different groups in an independent cluster within the family Micrococcaceae.
31274399	8	21	contain	contained	1134:1142	arg2	phosphatidylinositol					1211:1230	phosphatidylinositol	1211:1230	phosphatidylinositol	1211:1230	The menaquinones MK-9(H2) and MK-7(H2) were present in all of the isolates and the polar lipid pattern contained the phospholipids diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol and a glycolipid.
31274399	8	21	contain	contained	1134:1142	arg2	phosphatidylglycerol					1186:1205	phosphatidylglycerol	1186:1205	phosphatidylglycerol	1186:1205	The menaquinones MK-9(H2) and MK-7(H2) were present in all of the isolates and the polar lipid pattern contained the phospholipids diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol and a glycolipid.
31274399	8	21	contain	contained	1134:1142	arg2	phospholipids					1148:1160	the phospholipids diphosphatidylglycerol	1144:1183	the phospholipids diphosphatidylglycerol	1144:1183	The menaquinones MK-9(H2) and MK-7(H2) were present in all of the isolates and the polar lipid pattern contained the phospholipids diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol and a glycolipid.
31274399	8	21	contain	contained	1134:1142	arg1	pattern					1126:1132	the polar lipid pattern	1110:1132	the polar lipid pattern	1110:1132	The menaquinones MK-9(H2) and MK-7(H2) were present in all of the isolates and the polar lipid pattern contained the phospholipids diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol and a glycolipid.
31274399	11	22	theme	novel	1645:1649	arg1	genus					1651:1655	a novel genus	1643:1655	a novel genus	1643:1655	The results of phylogenetic, phenotypic and chemotaxonomic analyses indicated that the isolates belonged to two novel species of a novel genus, for which the names Galactobacter caseinivorans gen. nov., sp.
31274399	7	23	theme	predominant	925:935	arg1	acid					943:946	predominant fatty acid	925:946	predominant fatty acid	925:946	Chemotaxonomic analyses determined anteiso-C15 : 0 as predominant fatty acid, but also large amounts of iso-C15 : 0, iso-C16 : 0 and iso-C17 : 0 were detected.
31274399	3	24	theme	JZ	285:286	arg1	R-35T					288:292	JZ R-35T	285:292	JZ R-35T	285:292	Four Gram-stain positive, rod-shaped bacterial isolates, strains JZ R-183T, JZ RK-117, DI-46 and JZ R-35T, were recovered from bulk tank raw cow's milk from three different dairy farms in Germany.
31274399	3	24	theme	JZ	285:286	arg1	strains					245:251	strains JZ R-183T, JZ RK-117, DI-46 and JZ R-35T	245:292	strains JZ R-183T, JZ RK-117, DI-46 and JZ R-35T	245:292	Four Gram-stain positive, rod-shaped bacterial isolates, strains JZ R-183T, JZ RK-117, DI-46 and JZ R-35T, were recovered from bulk tank raw cow's milk from three different dairy farms in Germany.
31274399	6	25	theme	independent	817:827	arg1	cluster					829:835	an independent cluster	814:835	an independent cluster within the family Micrococcaceae	814:868	Phylogenetic analysis of 16S rRNA, recA and gyrB genes revealed that these isolates formed two different groups in an independent cluster within the family Micrococcaceae.
31274399	4	26	theme	sequences	417:425	arg1	Analysis					385:392	Analysis	385:392	Analysis of their 16S rRNA gene sequences	385:425	Analysis of their 16S rRNA gene sequences indicated that these isolates belonged to the family Micrococcaceae, closely related to the genera Arthrobacter, Neomicrococcus,Glutamicibacter and Citricoccus.
31274399	5	27	theme	type	668:671	arg1	strains					673:679	the next related type strains	651:679	the next related type strains	651:679	The 16S rRNA gene sequence similarity between the isolates and the next related type strains was below 97.3 %.
31274399	1	28	theme	Galactobacter	52:64	arg1	valiniphilus					66:77	nov. and Galactobacter valiniphilus	43:77	nov. and Galactobacter valiniphilus	43:77	nov. and Galactobacter valiniphilus sp.
31274399	3	29	theme	raw	325:327	arg1	cow					329:331	bulk tank raw cow's	315:333	bulk tank raw cow's milk from three different dairy farms in Germany	315:382	Four Gram-stain positive, rod-shaped bacterial isolates, strains JZ R-183T, JZ RK-117, DI-46 and JZ R-35T, were recovered from bulk tank raw cow's milk from three different dairy farms in Germany.
31274399	4	30	theme	rRNA	407:410	arg1	sequences					417:425	their 16S rRNA gene sequences	397:425	their 16S rRNA gene sequences	397:425	Analysis of their 16S rRNA gene sequences indicated that these isolates belonged to the family Micrococcaceae, closely related to the genera Arthrobacter, Neomicrococcus,Glutamicibacter and Citricoccus.
31274399	3	31	theme	different	351:359	arg1	farms					367:371	three different dairy farms	345:371	three different dairy farms in Germany	345:382	Four Gram-stain positive, rod-shaped bacterial isolates, strains JZ R-183T, JZ RK-117, DI-46 and JZ R-35T, were recovered from bulk tank raw cow's milk from three different dairy farms in Germany.
31274399	14	32	theme	107699T=LMG	1856:1866	arg1	30901T					1868:1873	=DSM 107699T=LMG 30901T	1851:1873	=DSM 107699T=LMG 30901T	1851:1873	The type strains are JZ R-183T (=DSM 107700T=LMG 30902T) and JZ R-35T (=DSM 107699T=LMG 30901T).
31274399	14	32	theme	107699T=LMG	1856:1866	arg1	R-35T					1844:1848	JZ R-35T	1841:1848	JZ R-35T (=DSM 107699T=LMG 30901T)	1841:1874	The type strains are JZ R-183T (=DSM 107700T=LMG 30902T) and JZ R-35T (=DSM 107699T=LMG 30901T).
31274399	3	33	theme	bulk	315:318	arg1	cow					329:331	bulk tank raw cow's	315:333	bulk tank raw cow's milk from three different dairy farms in Germany	315:382	Four Gram-stain positive, rod-shaped bacterial isolates, strains JZ R-183T, JZ RK-117, DI-46 and JZ R-35T, were recovered from bulk tank raw cow's milk from three different dairy farms in Germany.
31274399	4	34	theme	family	473:478	arg1	Micrococcaceae					480:493	the family Micrococcaceae	469:493	the family Micrococcaceae	469:493	Analysis of their 16S rRNA gene sequences indicated that these isolates belonged to the family Micrococcaceae, closely related to the genera Arthrobacter, Neomicrococcus,Glutamicibacter and Citricoccus.
31274399	10	35	theme	fatty	1374:1378	arg1	acid					1380:1383	The fatty acid	1370:1383	The fatty acid	1370:1383	The fatty acid and menaquinone profile differentiated the strains from the genera Arthrobacter, Neomicrococcus,Citricoccus and Glutamicibacter.
31274399	0	36	theme	gen.	28:31	arg1	sp					39:40	sp	39:40	sp	39:40	Galactobacter caseinivorans gen. nov., sp.
31274399	0	36	theme	gen.	28:31	arg1	nov.					33:36	gen. nov.	28:36	gen. nov.	28:36	Galactobacter caseinivorans gen. nov., sp.
31274399	2	37	theme	bacterial	156:164	arg1	cow					176:178	high bacterial count raw cow's	151:180	high bacterial count raw cow's milk	151:185	nov., two novel species of the family Micrococcaceae, isolated from high bacterial count raw cow's milk.
31274399	14	38	theme	JZ	1841:1842	arg1	30901T					1868:1873	=DSM 107699T=LMG 30901T	1851:1873	=DSM 107699T=LMG 30901T	1851:1873	The type strains are JZ R-183T (=DSM 107700T=LMG 30902T) and JZ R-35T (=DSM 107699T=LMG 30901T).
31274399	14	38	theme	JZ	1841:1842	arg1	R-35T					1844:1848	JZ R-35T	1841:1848	JZ R-35T (=DSM 107699T=LMG 30901T)	1841:1874	The type strains are JZ R-183T (=DSM 107700T=LMG 30902T) and JZ R-35T (=DSM 107699T=LMG 30901T).
31274399	14	39	theme	type	1784:1787	arg1	strains					1789:1795	The type strains	1780:1795	The type strains	1780:1795	The type strains are JZ R-183T (=DSM 107700T=LMG 30902T) and JZ R-35T (=DSM 107699T=LMG 30901T).
31274399	14	39	theme	type	1784:1787	arg1	R-183T					1804:1809	JZ R-183T	1801:1809	JZ R-183T (=DSM 107700T=LMG 30902T)	1801:1835	The type strains are JZ R-183T (=DSM 107700T=LMG 30902T) and JZ R-35T (=DSM 107699T=LMG 30901T).
31274399	12	40	theme	nov.	1721:1724	arg1	valiniphilus					1744:1755	nov. and Galactobacter valiniphilus	1721:1755	nov. and Galactobacter valiniphilus	1721:1755	nov. and Galactobacter valiniphilus sp.
31274399	12	41	theme	Galactobacter	1730:1742	arg1	valiniphilus					1744:1755	nov. and Galactobacter valiniphilus	1721:1755	nov. and Galactobacter valiniphilus	1721:1755	nov. and Galactobacter valiniphilus sp.
31274399	6	42	theme	genes	748:752	arg1	analysis					712:719	Phylogenetic analysis	699:719	Phylogenetic analysis of 16S rRNA, recA and gyrB genes	699:752	Phylogenetic analysis of 16S rRNA, recA and gyrB genes revealed that these isolates formed two different groups in an independent cluster within the family Micrococcaceae.
31274399	8	43	dep	phospholipids	1148:1160	arg1	diphosphatidylglycerol					1162:1183	diphosphatidylglycerol	1162:1183	the phospholipids diphosphatidylglycerol	1144:1183	The menaquinones MK-9(H2) and MK-7(H2) were present in all of the isolates and the polar lipid pattern contained the phospholipids diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol and a glycolipid.
31274399	2	44	theme	raw	172:174	arg1	cow					176:178	high bacterial count raw cow's	151:180	high bacterial count raw cow's milk	151:185	nov., two novel species of the family Micrococcaceae, isolated from high bacterial count raw cow's milk.
31274399	9	45	with	A4α	1293:1295	arg1	glutamate					1323:1331	glutamate	1323:1331	glutamate	1323:1331	The peptidoglycan type of the isolates was A4α, with alanine, lysine and glutamate as dominating cell wall amino acids.
31274399	9	45	with	A4α	1293:1295	arg1	lysine					1312:1317	lysine	1312:1317	lysine	1312:1317	The peptidoglycan type of the isolates was A4α, with alanine, lysine and glutamate as dominating cell wall amino acids.
31274399	9	45	with	A4α	1293:1295	arg1	alanine					1303:1309	alanine	1303:1309	alanine	1303:1309	The peptidoglycan type of the isolates was A4α, with alanine, lysine and glutamate as dominating cell wall amino acids.
31274399	1	46	theme	nov.	43:46	arg1	valiniphilus					66:77	nov. and Galactobacter valiniphilus	43:77	nov. and Galactobacter valiniphilus	43:77	nov. and Galactobacter valiniphilus sp.
31274399	11	47	theme	phenotypic	1543:1552	arg1	analyses					1573:1580	phylogenetic, phenotypic and chemotaxonomic analyses	1529:1580	phylogenetic, phenotypic and chemotaxonomic analyses	1529:1580	The results of phylogenetic, phenotypic and chemotaxonomic analyses indicated that the isolates belonged to two novel species of a novel genus, for which the names Galactobacter caseinivorans gen. nov., sp.
31274399	11	48	theme	chemotaxonomic	1558:1571	arg1	analyses					1573:1580	phylogenetic, phenotypic and chemotaxonomic analyses	1529:1580	phylogenetic, phenotypic and chemotaxonomic analyses	1529:1580	The results of phylogenetic, phenotypic and chemotaxonomic analyses indicated that the isolates belonged to two novel species of a novel genus, for which the names Galactobacter caseinivorans gen. nov., sp.
31274399	2	49	theme	novel	93:97	arg1	nov.					83:86	nov.	83:86	nov.	83:86	nov., two novel species of the family Micrococcaceae, isolated from high bacterial count raw cow's milk.
31274399	2	49	theme	novel	93:97	arg1	species					99:105	two novel species	89:105	two novel species of the family Micrococcaceae	89:134	nov., two novel species of the family Micrococcaceae, isolated from high bacterial count raw cow's milk.
31274399	5	50	theme	rRNA	596:599	arg1	similarity					615:624	The 16S rRNA gene sequence similarity	588:624	The 16S rRNA gene sequence similarity between the isolates and the next related type strains	588:679	The 16S rRNA gene sequence similarity between the isolates and the next related type strains was below 97.3 %.
31274399	6	51	theme	recA	734:737	arg1	analysis					712:719	Phylogenetic analysis	699:719	Phylogenetic analysis of 16S rRNA, recA and gyrB genes	699:752	Phylogenetic analysis of 16S rRNA, recA and gyrB genes revealed that these isolates formed two different groups in an independent cluster within the family Micrococcaceae.
31274399	8	52	from	all	1086:1088	arg1	menaquinones					1035:1046	The menaquinones MK-9(H2) and MK-7(H2)	1031:1068	The menaquinones MK-9(H2) and MK-7(H2)	1031:1068	The menaquinones MK-9(H2) and MK-7(H2) were present in all of the isolates and the polar lipid pattern contained the phospholipids diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol and a glycolipid.
31274399	8	52	from	all	1086:1088	arg1	present					1075:1081	present	1075:1081	present	1075:1081	The menaquinones MK-9(H2) and MK-7(H2) were present in all of the isolates and the polar lipid pattern contained the phospholipids diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol and a glycolipid.
31274399	6	53	theme	rRNA	728:731	arg1	analysis					712:719	Phylogenetic analysis	699:719	Phylogenetic analysis of 16S rRNA, recA and gyrB genes	699:752	Phylogenetic analysis of 16S rRNA, recA and gyrB genes revealed that these isolates formed two different groups in an independent cluster within the family Micrococcaceae.
31274399	8	54	from	present	1075:1081	arg1	isolates					1097:1104	the isolates	1093:1104	the isolates	1093:1104	The menaquinones MK-9(H2) and MK-7(H2) were present in all of the isolates and the polar lipid pattern contained the phospholipids diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol and a glycolipid.
31274399	8	54	from	present	1075:1081	arg1	all					1086:1088	all	1086:1088	all	1086:1088	The menaquinones MK-9(H2) and MK-7(H2) were present in all of the isolates and the polar lipid pattern contained the phospholipids diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol and a glycolipid.
31274399	2	55	theme	family	114:119	arg1	Micrococcaceae					121:134	the family Micrococcaceae	110:134	the family Micrococcaceae	110:134	nov., two novel species of the family Micrococcaceae, isolated from high bacterial count raw cow's milk.
31274399	3	56	theme	rod-shaped	214:223	arg1	Four					188:191	Four	188:191	Four	188:191	Four Gram-stain positive, rod-shaped bacterial isolates, strains JZ R-183T, JZ RK-117, DI-46 and JZ R-35T, were recovered from bulk tank raw cow's milk from three different dairy farms in Germany.
31274399	3	56	theme	rod-shaped	214:223	arg1	isolates					235:242	rod-shaped bacterial isolates	214:242	rod-shaped bacterial isolates	214:242	Four Gram-stain positive, rod-shaped bacterial isolates, strains JZ R-183T, JZ RK-117, DI-46 and JZ R-35T, were recovered from bulk tank raw cow's milk from three different dairy farms in Germany.
31274399	11	57	theme	gen.	1706:1709	arg1	sp					1717:1718	sp	1717:1718	sp	1717:1718	The results of phylogenetic, phenotypic and chemotaxonomic analyses indicated that the isolates belonged to two novel species of a novel genus, for which the names Galactobacter caseinivorans gen. nov., sp.
31274399	11	57	theme	gen.	1706:1709	arg1	nov.					1711:1714	Galactobacter caseinivorans gen. nov.	1678:1714	Galactobacter caseinivorans gen. nov.	1678:1714	The results of phylogenetic, phenotypic and chemotaxonomic analyses indicated that the isolates belonged to two novel species of a novel genus, for which the names Galactobacter caseinivorans gen. nov., sp.
31274399	6	58	theme	Phylogenetic	699:710	arg1	analysis					712:719	Phylogenetic analysis	699:719	Phylogenetic analysis of 16S rRNA, recA and gyrB genes	699:752	Phylogenetic analysis of 16S rRNA, recA and gyrB genes revealed that these isolates formed two different groups in an independent cluster within the family Micrococcaceae.
31274399	9	59	theme	dominating	1336:1345	arg1	wall					1352:1355	dominating cell wall	1336:1355	dominating cell wall amino acids	1336:1367	The peptidoglycan type of the isolates was A4α, with alanine, lysine and glutamate as dominating cell wall amino acids.
31274399	11	60	theme	Galactobacter	1678:1690	arg1	sp					1717:1718	sp	1717:1718	sp	1717:1718	The results of phylogenetic, phenotypic and chemotaxonomic analyses indicated that the isolates belonged to two novel species of a novel genus, for which the names Galactobacter caseinivorans gen. nov., sp.
31274399	11	60	theme	Galactobacter	1678:1690	arg1	nov.					1711:1714	Galactobacter caseinivorans gen. nov.	1678:1714	Galactobacter caseinivorans gen. nov.	1678:1714	The results of phylogenetic, phenotypic and chemotaxonomic analyses indicated that the isolates belonged to two novel species of a novel genus, for which the names Galactobacter caseinivorans gen. nov., sp.
31274399	9	61	theme	wall	1352:1355	arg1	acids					1363:1367	dominating cell wall amino acids	1336:1367	dominating cell wall amino acids	1336:1367	The peptidoglycan type of the isolates was A4α, with alanine, lysine and glutamate as dominating cell wall amino acids.
31274399	8	62	theme	lipid	1120:1124	arg1	pattern					1126:1132	the polar lipid pattern	1110:1132	the polar lipid pattern	1110:1132	The menaquinones MK-9(H2) and MK-7(H2) were present in all of the isolates and the polar lipid pattern contained the phospholipids diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol and a glycolipid.
31274399	5	63	theme	sequence	606:613	arg1	similarity					615:624	The 16S rRNA gene sequence similarity	588:624	The 16S rRNA gene sequence similarity between the isolates and the next related type strains	588:679	The 16S rRNA gene sequence similarity between the isolates and the next related type strains was below 97.3 %.
31274399	1	64	dep	sp	79:80	arg1	valiniphilus					66:77	nov. and Galactobacter valiniphilus	43:77	nov. and Galactobacter valiniphilus	43:77	nov. and Galactobacter valiniphilus sp.
31274399	7	65	theme	large	958:962	arg1	iso-C15 					975:982	iso-C15 	975:982	iso-C15 	975:982	Chemotaxonomic analyses determined anteiso-C15 : 0 as predominant fatty acid, but also large amounts of iso-C15 : 0, iso-C16 : 0 and iso-C17 : 0 were detected.
31274399	7	65	theme	large	958:962	arg1	amounts					964:970	large amounts	958:970	large amounts of iso-C15 	958:982	Chemotaxonomic analyses determined anteiso-C15 : 0 as predominant fatty acid, but also large amounts of iso-C15 : 0, iso-C16 : 0 and iso-C17 : 0 were detected.
31274399	8	66	attach	present	1075:1081	arg1	isolates					1097:1104	the isolates	1093:1104	the isolates	1093:1104	The menaquinones MK-9(H2) and MK-7(H2) were present in all of the isolates and the polar lipid pattern contained the phospholipids diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol and a glycolipid.
31274399	8	66	attach	present	1075:1081	arg2	MK-9					1048:1051	MK-9	1048:1051	MK-9	1048:1051	The menaquinones MK-9(H2) and MK-7(H2) were present in all of the isolates and the polar lipid pattern contained the phospholipids diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol and a glycolipid.
31274399	8	66	attach	present	1075:1081	arg2	menaquinones					1035:1046	The menaquinones MK-9(H2) and MK-7(H2)	1031:1068	The menaquinones MK-9(H2) and MK-7(H2)	1031:1068	The menaquinones MK-9(H2) and MK-7(H2) were present in all of the isolates and the polar lipid pattern contained the phospholipids diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol and a glycolipid.
31274399	8	66	attach	present	1075:1081	arg2	MK-7					1061:1064	MK-7	1061:1064	MK-7	1061:1064	The menaquinones MK-9(H2) and MK-7(H2) were present in all of the isolates and the polar lipid pattern contained the phospholipids diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol and a glycolipid.
31274399	8	66	attach	present	1075:1081	arg1	all					1086:1088	all	1086:1088	all	1086:1088	The menaquinones MK-9(H2) and MK-7(H2) were present in all of the isolates and the polar lipid pattern contained the phospholipids diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol and a glycolipid.
31274399	8	66	attach	present	1075:1081	arg2	present					1075:1081	present	1075:1081	present	1075:1081	The menaquinones MK-9(H2) and MK-7(H2) were present in all of the isolates and the polar lipid pattern contained the phospholipids diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol and a glycolipid.
31274399	7	67	dep	anteiso-C15 	906:917	arg1	 0					984:985	 0	984:985	 0	984:985	Chemotaxonomic analyses determined anteiso-C15 : 0 as predominant fatty acid, but also large amounts of iso-C15 : 0, iso-C16 : 0 and iso-C17 : 0 were detected.
31274399	7	67	dep	anteiso-C15 	906:917	arg1	 0					919:920	 0	919:920	anteiso-C15 : 0 as predominant fatty acid, but also large amounts of iso-C15 : 0, iso-C16 : 0 and iso-C17 	906:1011	Chemotaxonomic analyses determined anteiso-C15 : 0 as predominant fatty acid, but also large amounts of iso-C15 : 0, iso-C16 : 0 and iso-C17 : 0 were detected.
31274399	7	67	dep	anteiso-C15 	906:917	arg1	iso-C17 					1004:1011	iso-C17 	1004:1011	iso-C17 	1004:1011	Chemotaxonomic analyses determined anteiso-C15 : 0 as predominant fatty acid, but also large amounts of iso-C15 : 0, iso-C16 : 0 and iso-C17 : 0 were detected.
31274399	7	67	dep	anteiso-C15 	906:917	arg1	 0					997:998	 0	997:998	 0	997:998	Chemotaxonomic analyses determined anteiso-C15 : 0 as predominant fatty acid, but also large amounts of iso-C15 : 0, iso-C16 : 0 and iso-C17 : 0 were detected.
31274399	7	67	dep	anteiso-C15 	906:917	arg1	iso-C16 					988:995	iso-C16 	988:995	iso-C16 	988:995	Chemotaxonomic analyses determined anteiso-C15 : 0 as predominant fatty acid, but also large amounts of iso-C15 : 0, iso-C16 : 0 and iso-C17 : 0 were detected.
31274399	11	68	theme	genus	1651:1655	arg1	species					1632:1638	two novel species	1622:1638	two novel species of a novel genus, for which the names Galactobacter caseinivorans gen. nov., sp	1622:1718	The results of phylogenetic, phenotypic and chemotaxonomic analyses indicated that the isolates belonged to two novel species of a novel genus, for which the names Galactobacter caseinivorans gen. nov., sp.
31274399	7	69	theme	fatty	937:941	arg1	acid					943:946	predominant fatty acid	925:946	predominant fatty acid	925:946	Chemotaxonomic analyses determined anteiso-C15 : 0 as predominant fatty acid, but also large amounts of iso-C15 : 0, iso-C16 : 0 and iso-C17 : 0 were detected.
31274399	5	70	theme	related	660:666	arg1	strains					673:679	the next related type strains	651:679	the next related type strains	651:679	The 16S rRNA gene sequence similarity between the isolates and the next related type strains was below 97.3 %.
31274399	14	71	theme	107700T=LMG	1817:1827	arg1	30902T					1829:1834	=DSM 107700T=LMG 30902T	1812:1834	=DSM 107700T=LMG 30902T	1812:1834	The type strains are JZ R-183T (=DSM 107700T=LMG 30902T) and JZ R-35T (=DSM 107699T=LMG 30901T).
31274399	14	71	theme	107700T=LMG	1817:1827	arg1	R-183T					1804:1809	JZ R-183T	1801:1809	JZ R-183T (=DSM 107700T=LMG 30902T)	1801:1835	The type strains are JZ R-183T (=DSM 107700T=LMG 30902T) and JZ R-35T (=DSM 107699T=LMG 30901T).
31274399	4	72	theme	16S	403:405	arg1	sequences					417:425	their 16S rRNA gene sequences	397:425	their 16S rRNA gene sequences	397:425	Analysis of their 16S rRNA gene sequences indicated that these isolates belonged to the family Micrococcaceae, closely related to the genera Arthrobacter, Neomicrococcus,Glutamicibacter and Citricoccus.
31274399	4	73	theme	gene	412:415	arg1	sequences					417:425	their 16S rRNA gene sequences	397:425	their 16S rRNA gene sequences	397:425	Analysis of their 16S rRNA gene sequences indicated that these isolates belonged to the family Micrococcaceae, closely related to the genera Arthrobacter, Neomicrococcus,Glutamicibacter and Citricoccus.
31274399	8	74	dep	menaquinones	1035:1046	arg1	H2					1066:1067	H2	1066:1067	H2	1066:1067	The menaquinones MK-9(H2) and MK-7(H2) were present in all of the isolates and the polar lipid pattern contained the phospholipids diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol and a glycolipid.
31274399	8	74	dep	menaquinones	1035:1046	arg1	H2					1053:1054	H2	1053:1054	H2	1053:1054	The menaquinones MK-9(H2) and MK-7(H2) were present in all of the isolates and the polar lipid pattern contained the phospholipids diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol and a glycolipid.
31274399	8	74	dep	menaquinones	1035:1046	arg1	MK-7					1061:1064	MK-7	1061:1064	MK-7	1061:1064	The menaquinones MK-9(H2) and MK-7(H2) were present in all of the isolates and the polar lipid pattern contained the phospholipids diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol and a glycolipid.
31274399	8	74	dep	menaquinones	1035:1046	arg1	MK-9					1048:1051	MK-9	1048:1051	MK-9	1048:1051	The menaquinones MK-9(H2) and MK-7(H2) were present in all of the isolates and the polar lipid pattern contained the phospholipids diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol and a glycolipid.
31274399	8	74	dep	menaquinones	1035:1046	arg1	menaquinones					1035:1046	The menaquinones MK-9(H2) and MK-7(H2)	1031:1068	The menaquinones MK-9(H2) and MK-7(H2)	1031:1068	The menaquinones MK-9(H2) and MK-7(H2) were present in all of the isolates and the polar lipid pattern contained the phospholipids diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol and a glycolipid.
31274399	7	75	theme	Chemotaxonomic	871:884	arg1	analyses					886:893	Chemotaxonomic analyses	871:893	Chemotaxonomic analyses	871:893	Chemotaxonomic analyses determined anteiso-C15 : 0 as predominant fatty acid, but also large amounts of iso-C15 : 0, iso-C16 : 0 and iso-C17 : 0 were detected.
31274399	6	76	theme	gyrB	743:746	arg1	genes					748:752	gyrB genes	743:752	gyrB genes	743:752	Phylogenetic analysis of 16S rRNA, recA and gyrB genes revealed that these isolates formed two different groups in an independent cluster within the family Micrococcaceae.
31274399	3	77	theme	dairy	361:365	arg1	farms					367:371	three different dairy farms	345:371	three different dairy farms in Germany	345:382	Four Gram-stain positive, rod-shaped bacterial isolates, strains JZ R-183T, JZ RK-117, DI-46 and JZ R-35T, were recovered from bulk tank raw cow's milk from three different dairy farms in Germany.
31274399	14	78	theme	=DSM	1851:1854	arg1	30901T					1868:1873	=DSM 107699T=LMG 30901T	1851:1873	=DSM 107699T=LMG 30901T	1851:1873	The type strains are JZ R-183T (=DSM 107700T=LMG 30902T) and JZ R-35T (=DSM 107699T=LMG 30901T).
31274399	14	78	theme	=DSM	1851:1854	arg1	R-35T					1844:1848	JZ R-35T	1841:1848	JZ R-35T (=DSM 107699T=LMG 30901T)	1841:1874	The type strains are JZ R-183T (=DSM 107700T=LMG 30902T) and JZ R-35T (=DSM 107699T=LMG 30901T).
31274399	3	79	theme	tank	320:323	arg1	cow					329:331	bulk tank raw cow's	315:333	bulk tank raw cow's milk from three different dairy farms in Germany	315:382	Four Gram-stain positive, rod-shaped bacterial isolates, strains JZ R-183T, JZ RK-117, DI-46 and JZ R-35T, were recovered from bulk tank raw cow's milk from three different dairy farms in Germany.
31274399	2	80	theme	count	166:170	arg1	cow					176:178	high bacterial count raw cow's	151:180	high bacterial count raw cow's milk	151:185	nov., two novel species of the family Micrococcaceae, isolated from high bacterial count raw cow's milk.
31274399	2	81	theme	high	151:154	arg1	cow					176:178	high bacterial count raw cow's	151:180	high bacterial count raw cow's milk	151:185	nov., two novel species of the family Micrococcaceae, isolated from high bacterial count raw cow's milk.
31274399	9	82	theme	peptidoglycan	1254:1266	arg1	A4α					1293:1295	A4α	1293:1295	A4α	1293:1295	The peptidoglycan type of the isolates was A4α, with alanine, lysine and glutamate as dominating cell wall amino acids.
31274399	9	82	theme	peptidoglycan	1254:1266	arg1	type					1268:1271	The peptidoglycan type	1250:1271	The peptidoglycan type of the isolates	1250:1287	The peptidoglycan type of the isolates was A4α, with alanine, lysine and glutamate as dominating cell wall amino acids.
31274399	10	83	theme	menaquinone	1389:1399	arg1	profile					1401:1407	menaquinone profile	1389:1407	menaquinone profile	1389:1407	The fatty acid and menaquinone profile differentiated the strains from the genera Arthrobacter, Neomicrococcus,Citricoccus and Glutamicibacter.
31274399	11	84	theme	novel	1626:1630	arg1	species					1632:1638	two novel species	1622:1638	two novel species of a novel genus, for which the names Galactobacter caseinivorans gen. nov., sp	1622:1718	The results of phylogenetic, phenotypic and chemotaxonomic analyses indicated that the isolates belonged to two novel species of a novel genus, for which the names Galactobacter caseinivorans gen. nov., sp.
31274399	8	85	located	present	1075:1081	arg1	isolates					1097:1104	the isolates	1093:1104	the isolates	1093:1104	The menaquinones MK-9(H2) and MK-7(H2) were present in all of the isolates and the polar lipid pattern contained the phospholipids diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol and a glycolipid.
31274399	8	85	located	present	1075:1081	arg2	MK-9					1048:1051	MK-9	1048:1051	MK-9	1048:1051	The menaquinones MK-9(H2) and MK-7(H2) were present in all of the isolates and the polar lipid pattern contained the phospholipids diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol and a glycolipid.
31274399	8	85	located	present	1075:1081	arg2	menaquinones					1035:1046	The menaquinones MK-9(H2) and MK-7(H2)	1031:1068	The menaquinones MK-9(H2) and MK-7(H2)	1031:1068	The menaquinones MK-9(H2) and MK-7(H2) were present in all of the isolates and the polar lipid pattern contained the phospholipids diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol and a glycolipid.
31274399	8	85	located	present	1075:1081	arg2	MK-7					1061:1064	MK-7	1061:1064	MK-7	1061:1064	The menaquinones MK-9(H2) and MK-7(H2) were present in all of the isolates and the polar lipid pattern contained the phospholipids diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol and a glycolipid.
31274399	8	85	located	present	1075:1081	arg1	all					1086:1088	all	1086:1088	all	1086:1088	The menaquinones MK-9(H2) and MK-7(H2) were present in all of the isolates and the polar lipid pattern contained the phospholipids diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol and a glycolipid.
31274399	8	85	located	present	1075:1081	arg2	present					1075:1081	present	1075:1081	present	1075:1081	The menaquinones MK-9(H2) and MK-7(H2) were present in all of the isolates and the polar lipid pattern contained the phospholipids diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol and a glycolipid.
31274399	9	86	theme	isolates	1280:1287	arg1	A4α					1293:1295	A4α	1293:1295	A4α	1293:1295	The peptidoglycan type of the isolates was A4α, with alanine, lysine and glutamate as dominating cell wall amino acids.
31274399	9	86	theme	isolates	1280:1287	arg1	type					1268:1271	The peptidoglycan type	1250:1271	The peptidoglycan type of the isolates	1250:1287	The peptidoglycan type of the isolates was A4α, with alanine, lysine and glutamate as dominating cell wall amino acids.
31274399	10	87	from	Arthrobacter	1452:1463	arg1	strains					1428:1434	the strains	1424:1434	the strains from the genera Arthrobacter, Neomicrococcus,Citricoccus and Glutamicibacter	1424:1511	The fatty acid and menaquinone profile differentiated the strains from the genera Arthrobacter, Neomicrococcus,Citricoccus and Glutamicibacter.
31274399	14	88	theme	=DSM	1812:1815	arg1	30902T					1829:1834	=DSM 107700T=LMG 30902T	1812:1834	=DSM 107700T=LMG 30902T	1812:1834	The type strains are JZ R-183T (=DSM 107700T=LMG 30902T) and JZ R-35T (=DSM 107699T=LMG 30901T).
31274399	14	88	theme	=DSM	1812:1815	arg1	R-183T					1804:1809	JZ R-183T	1801:1809	JZ R-183T (=DSM 107700T=LMG 30902T)	1801:1835	The type strains are JZ R-183T (=DSM 107700T=LMG 30902T) and JZ R-35T (=DSM 107699T=LMG 30901T).
31274399	3	89	dep	strains	245:251	arg1	JZ					264:265	JZ RK-117	264:272	JZ RK-117	264:272	Four Gram-stain positive, rod-shaped bacterial isolates, strains JZ R-183T, JZ RK-117, DI-46 and JZ R-35T, were recovered from bulk tank raw cow's milk from three different dairy farms in Germany.
31274399	3	89	dep	strains	245:251	arg1	DI-46					275:279	DI-46	275:279	DI-46	275:279	Four Gram-stain positive, rod-shaped bacterial isolates, strains JZ R-183T, JZ RK-117, DI-46 and JZ R-35T, were recovered from bulk tank raw cow's milk from three different dairy farms in Germany.
31274399	3	89	dep	strains	245:251	arg1	R-35T					288:292	JZ R-35T	285:292	JZ R-35T	285:292	Four Gram-stain positive, rod-shaped bacterial isolates, strains JZ R-183T, JZ RK-117, DI-46 and JZ R-35T, were recovered from bulk tank raw cow's milk from three different dairy farms in Germany.
31274399	3	89	dep	strains	245:251	arg1	strains					245:251	strains JZ R-183T, JZ RK-117, DI-46 and JZ R-35T	245:292	strains JZ R-183T, JZ RK-117, DI-46 and JZ R-35T	245:292	Four Gram-stain positive, rod-shaped bacterial isolates, strains JZ R-183T, JZ RK-117, DI-46 and JZ R-35T, were recovered from bulk tank raw cow's milk from three different dairy farms in Germany.
31274399	3	89	dep	strains	245:251	arg1	R-183T					256:261	JZ R-183T	253:261	JZ R-183T	253:261	Four Gram-stain positive, rod-shaped bacterial isolates, strains JZ R-183T, JZ RK-117, DI-46 and JZ R-35T, were recovered from bulk tank raw cow's milk from three different dairy farms in Germany.
31274399	3	90	from	farms	367:371	arg1	milk					335:338	bulk tank raw cow's milk	315:338	bulk tank raw cow's milk from three different dairy farms in Germany	315:382	Four Gram-stain positive, rod-shaped bacterial isolates, strains JZ R-183T, JZ RK-117, DI-46 and JZ R-35T, were recovered from bulk tank raw cow's milk from three different dairy farms in Germany.
31274399	3	90	from	farms	367:371	arg1	Germany					376:382	Germany	376:382	Germany	376:382	Four Gram-stain positive, rod-shaped bacterial isolates, strains JZ R-183T, JZ RK-117, DI-46 and JZ R-35T, were recovered from bulk tank raw cow's milk from three different dairy farms in Germany.
30351271	3	0	theme	optimum	271:277	arg1	10-40 °C					261:268	10-40 °C	261:268	10-40 °C (optimum 28 °C)	261:284	The isolate grew at 10-40 °C (optimum 28 °C), at pH 5.0-10.0 (optimum pH 7.0) and in the presence of up to 5 % (w/v) NaCl, although NaCl was not required for growth.
30351271	3	0	theme	optimum	271:277	arg1	28 °C					279:283	optimum 28 °C	271:283	optimum 28 °C	271:283	The isolate grew at 10-40 °C (optimum 28 °C), at pH 5.0-10.0 (optimum pH 7.0) and in the presence of up to 5 % (w/v) NaCl, although NaCl was not required for growth.
30351271	6	1	theme	hybridization	776:788	arg1	value					790:794	The in silico DNA-DNA hybridization value	754:794	The in silico DNA-DNA hybridization value between strain NEAU-SA1T and A. cupressi DSM 24664T	754:846	The in silico DNA-DNA hybridization value between strain NEAU-SA1T and A. cupressi DSM 24664T was 34.20 %, again indicating they belong to different taxa.
30351271	8	2	dep	anteiso-C15 	997:1008	arg1	 0					1027:1028	 0	1027:1028	 0	1027:1028	The major cellular fatty acids (>10 %) were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
30351271	8	2	dep	anteiso-C15 	997:1008	arg1	 0					1043:1044	 0	1043:1044	 0	1043:1044	The major cellular fatty acids (>10 %) were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
30351271	8	2	dep	anteiso-C15 	997:1008	arg1	 0					1010:1011	 0	1010:1011	 0	1010:1011	The major cellular fatty acids (>10 %) were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
30351271	8	2	dep	anteiso-C15 	997:1008	arg1	iso-C16 					1034:1041	iso-C16 	1034:1041	iso-C16 	1034:1041	The major cellular fatty acids (>10 %) were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
30351271	15	3	theme	106716T=CCTCC	1634:1646	arg1	2017271T					1651:1658	=DSM 106716T=CCTCC AB 2017271T	1629:1658	=DSM 106716T=CCTCC AB 2017271T	1629:1658	The type strain is NEAU-SA1T (=DSM 106716T=CCTCC AB 2017271T).
30351271	15	3	theme	106716T=CCTCC	1634:1646	arg1	NEAU-SA1T					1618:1626	NEAU-SA1T	1618:1626	NEAU-SA1T (=DSM 106716T=CCTCC AB 2017271T)	1618:1659	The type strain is NEAU-SA1T (=DSM 106716T=CCTCC AB 2017271T).
30351271	7	4	theme	G+C	925:927	arg1	content					929:935	The genomic DNA G+C content	909:935	The genomic DNA G+C content	909:935	The genomic DNA G+C content was 66.74 mol%.
30351271	7	4	theme	G+C	925:927	arg1	%					950:950	66.74 mol%	941:950	66.74 mol%	941:950	The genomic DNA G+C content was 66.74 mol%.
30351271	6	5	theme	DNA-DNA	768:774	arg1	hybridization					776:788	The in silico DNA-DNA hybridization	754:788	The in silico DNA-DNA hybridization value between strain NEAU-SA1T and A. cupressi DSM 24664T	754:846	The in silico DNA-DNA hybridization value between strain NEAU-SA1T and A. cupressi DSM 24664T was 34.20 %, again indicating they belong to different taxa.
30351271	4	6	theme	gene	447:450	arg1	sequences					452:460	16S rRNA gene sequences	438:460	16S rRNA gene sequences	438:460	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NEAU-SA1T belonged to the genus Arthrobacter and was closely related to Arthrobacter cupressi DSM 24664T (98.1 % similarity).
30351271	1	7	theme	forest	47:52	arg1	soil					54:57	forest soil	47:57	forest soil	47:57	nov., isolated from forest soil.
30351271	6	8	theme	in	758:759	arg1	hybridization					776:788	The in silico DNA-DNA hybridization	754:788	The in silico DNA-DNA hybridization value between strain NEAU-SA1T and A. cupressi DSM 24664T	754:846	The in silico DNA-DNA hybridization value between strain NEAU-SA1T and A. cupressi DSM 24664T was 34.20 %, again indicating they belong to different taxa.
30351271	6	9	theme	strain	804:809	arg1	NEAU-SA1T					811:819	strain NEAU-SA1T	804:819	strain NEAU-SA1T	804:819	The in silico DNA-DNA hybridization value between strain NEAU-SA1T and A. cupressi DSM 24664T was 34.20 %, again indicating they belong to different taxa.
30351271	13	10	dep	analysis	1434:1441	arg1	the					1360:1362	the	1360:1362	the	1360:1362	On the basis of morphological, physiological, biochemical and chemotaxonomic analysis, strain NEAU-SA1T was classified as representing a novel species in the genus Arthrobacter, for which the name Arthrobacter silvisoli sp.
30351271	13	10	dep	analysis	1434:1441	arg1	basis					1364:1368	basis	1364:1368	basis	1364:1368	On the basis of morphological, physiological, biochemical and chemotaxonomic analysis, strain NEAU-SA1T was classified as representing a novel species in the genus Arthrobacter, for which the name Arthrobacter silvisoli sp.
30351271	5	11	theme	Average	610:616	arg1	values					638:643	Average nucleotide identity values	610:643	Average nucleotide identity values between NEAU-SA1T and A. cupressi DSM 24664T	610:688	Average nucleotide identity values between NEAU-SA1T and A. cupressi DSM 24664T were 88.91 and 87.41 % by ANIm and ANIb analysis, respectively.
30351271	7	12	theme	66.74 mol	941:949	arg1	content					929:935	The genomic DNA G+C content	909:935	The genomic DNA G+C content	909:935	The genomic DNA G+C content was 66.74 mol%.
30351271	7	12	theme	66.74 mol	941:949	arg1	%					950:950	66.74 mol%	941:950	66.74 mol%	941:950	The genomic DNA G+C content was 66.74 mol%.
30351271	15	13	theme	=DSM	1629:1632	arg1	2017271T					1651:1658	=DSM 106716T=CCTCC AB 2017271T	1629:1658	=DSM 106716T=CCTCC AB 2017271T	1629:1658	The type strain is NEAU-SA1T (=DSM 106716T=CCTCC AB 2017271T).
30351271	15	13	theme	=DSM	1629:1632	arg1	NEAU-SA1T					1618:1626	NEAU-SA1T	1618:1626	NEAU-SA1T (=DSM 106716T=CCTCC AB 2017271T)	1618:1659	The type strain is NEAU-SA1T (=DSM 106716T=CCTCC AB 2017271T).
30351271	2	14	attach	isolated	174:181	arg1	Hunan					218:222	Hunan	218:222	Hunan	218:222	A novel Gram-stain-positive, strictly aerobic strain, NEAU-SA1T, which showed a rod-coccus growth life cycle, was isolated from forest soil from Zhangjiajie, Hunan Province, China.
30351271	2	14	attach	isolated	174:181	arg1	China					234:238	China	234:238	China	234:238	A novel Gram-stain-positive, strictly aerobic strain, NEAU-SA1T, which showed a rod-coccus growth life cycle, was isolated from forest soil from Zhangjiajie, Hunan Province, China.
30351271	2	14	attach	isolated	174:181	arg2	NEAU-SA1T					114:122	NEAU-SA1T	114:122	NEAU-SA1T	114:122	A novel Gram-stain-positive, strictly aerobic strain, NEAU-SA1T, which showed a rod-coccus growth life cycle, was isolated from forest soil from Zhangjiajie, Hunan Province, China.
30351271	2	14	attach	isolated	174:181	arg1	soil					195:198	forest soil	188:198	forest soil	188:198	A novel Gram-stain-positive, strictly aerobic strain, NEAU-SA1T, which showed a rod-coccus growth life cycle, was isolated from forest soil from Zhangjiajie, Hunan Province, China.
30351271	2	14	attach	isolated	174:181	arg2	strain					106:111	A novel Gram-stain-positive, strictly aerobic strain	60:111	A novel Gram-stain-positive, strictly aerobic strain	60:111	A novel Gram-stain-positive, strictly aerobic strain, NEAU-SA1T, which showed a rod-coccus growth life cycle, was isolated from forest soil from Zhangjiajie, Hunan Province, China.
30351271	5	15	theme	nucleotide	618:627	arg1	values					638:643	Average nucleotide identity values	610:643	Average nucleotide identity values between NEAU-SA1T and A. cupressi DSM 24664T	610:688	Average nucleotide identity values between NEAU-SA1T and A. cupressi DSM 24664T were 88.91 and 87.41 % by ANIm and ANIb analysis, respectively.
30351271	9	16	theme	unidentified	1144:1155	arg1	glycolipids					1157:1167	two unidentified glycolipids	1140:1167	two unidentified glycolipids	1140:1167	The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and two unidentified glycolipids.
30351271	13	17	theme	strain	1444:1449	arg1	NEAU-SA1T					1451:1459	strain NEAU-SA1T	1444:1459	strain NEAU-SA1T	1444:1459	On the basis of morphological, physiological, biochemical and chemotaxonomic analysis, strain NEAU-SA1T was classified as representing a novel species in the genus Arthrobacter, for which the name Arthrobacter silvisoli sp.
30351271	5	18	theme	ANIb	725:728	arg1	analysis					730:737	ANIb analysis	725:737	ANIb analysis	725:737	Average nucleotide identity values between NEAU-SA1T and A. cupressi DSM 24664T were 88.91 and 87.41 % by ANIm and ANIb analysis, respectively.
30351271	0	19	theme	silvisoli	13:21	arg1	sp					23:24	Arthrobacter silvisoli sp	0:24	Arthrobacter silvisoli sp.	0:25	Arthrobacter silvisoli sp.
30351271	6	20	theme	A.	825:826	arg1	24664T					841:846	A. cupressi DSM 24664T	825:846	A. cupressi DSM 24664T	825:846	The in silico DNA-DNA hybridization value between strain NEAU-SA1T and A. cupressi DSM 24664T was 34.20 %, again indicating they belong to different taxa.
30351271	13	21	theme	genus	1515:1519	arg1	Arthrobacter					1521:1532	the genus Arthrobacter	1511:1532	the genus Arthrobacter	1511:1532	On the basis of morphological, physiological, biochemical and chemotaxonomic analysis, strain NEAU-SA1T was classified as representing a novel species in the genus Arthrobacter, for which the name Arthrobacter silvisoli sp.
30351271	13	22	theme	physiological	1388:1400	arg1	analysis					1434:1441	morphological, physiological, biochemical and chemotaxonomic analysis	1373:1441	morphological, physiological, biochemical and chemotaxonomic analysis	1373:1441	On the basis of morphological, physiological, biochemical and chemotaxonomic analysis, strain NEAU-SA1T was classified as representing a novel species in the genus Arthrobacter, for which the name Arthrobacter silvisoli sp.
30351271	11	23	theme	interpeptide	1251:1262	arg1	bridge					1264:1269	an interpeptide bridge	1248:1269	an interpeptide bridge comprising l-Lys and l-Ala	1248:1296	The peptidoglycan type was A3α with an interpeptide bridge comprising l-Lys and l-Ala.
30351271	0	24	theme	Arthrobacter	0:11	arg1	sp					23:24	Arthrobacter silvisoli sp	0:24	Arthrobacter silvisoli sp.	0:25	Arthrobacter silvisoli sp.
30351271	11	25	theme	peptidoglycan	1216:1228	arg1	type					1230:1233	The peptidoglycan type	1212:1233	The peptidoglycan type	1212:1233	The peptidoglycan type was A3α with an interpeptide bridge comprising l-Lys and l-Ala.
30351271	15	26	theme	type	1603:1606	arg1	NEAU-SA1T					1618:1626	NEAU-SA1T	1618:1626	NEAU-SA1T (=DSM 106716T=CCTCC AB 2017271T)	1618:1659	The type strain is NEAU-SA1T (=DSM 106716T=CCTCC AB 2017271T).
30351271	15	26	theme	type	1603:1606	arg1	strain					1608:1613	The type strain	1599:1613	The type strain	1599:1613	The type strain is NEAU-SA1T (=DSM 106716T=CCTCC AB 2017271T).
30351271	3	27	theme	optimum	303:309	arg1	pH					290:291	pH 5.0-10.0	290:300	pH 5.0-10.0 (optimum pH 7.0)	290:317	The isolate grew at 10-40 °C (optimum 28 °C), at pH 5.0-10.0 (optimum pH 7.0) and in the presence of up to 5 % (w/v) NaCl, although NaCl was not required for growth.
30351271	3	27	theme	optimum	303:309	arg1	pH					311:312	optimum pH 7.0	303:316	optimum pH 7.0	303:316	The isolate grew at 10-40 °C (optimum 28 °C), at pH 5.0-10.0 (optimum pH 7.0) and in the presence of up to 5 % (w/v) NaCl, although NaCl was not required for growth.
30351271	6	28	theme	DSM	837:839	arg1	24664T					841:846	A. cupressi DSM 24664T	825:846	A. cupressi DSM 24664T	825:846	The in silico DNA-DNA hybridization value between strain NEAU-SA1T and A. cupressi DSM 24664T was 34.20 %, again indicating they belong to different taxa.
30351271	7	29	theme	DNA	921:923	arg1	content					929:935	The genomic DNA G+C content	909:935	The genomic DNA G+C content	909:935	The genomic DNA G+C content was 66.74 mol%.
30351271	7	29	theme	DNA	921:923	arg1	%					950:950	66.74 mol%	941:950	66.74 mol%	941:950	The genomic DNA G+C content was 66.74 mol%.
30351271	2	30	theme	forest	188:193	arg1	soil					195:198	forest soil	188:198	forest soil	188:198	A novel Gram-stain-positive, strictly aerobic strain, NEAU-SA1T, which showed a rod-coccus growth life cycle, was isolated from forest soil from Zhangjiajie, Hunan Province, China.
30351271	6	31	theme	different	893:901	arg1	taxa					903:906	different taxa	893:906	different taxa	893:906	The in silico DNA-DNA hybridization value between strain NEAU-SA1T and A. cupressi DSM 24664T was 34.20 %, again indicating they belong to different taxa.
30351271	4	32	theme	genus	510:514	arg1	Arthrobacter					516:527	the genus Arthrobacter	506:527	the genus Arthrobacter	506:527	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NEAU-SA1T belonged to the genus Arthrobacter and was closely related to Arthrobacter cupressi DSM 24664T (98.1 % similarity).
30351271	4	33	theme	rRNA	442:445	arg1	sequences					452:460	16S rRNA gene sequences	438:460	16S rRNA gene sequences	438:460	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NEAU-SA1T belonged to the genus Arthrobacter and was closely related to Arthrobacter cupressi DSM 24664T (98.1 % similarity).
30351271	5	34	dep	NEAU-SA1T	653:661	arg1	24664T					683:688	DSM 24664T	679:688	DSM 24664T	679:688	Average nucleotide identity values between NEAU-SA1T and A. cupressi DSM 24664T were 88.91 and 87.41 % by ANIm and ANIb analysis, respectively.
30351271	13	35	theme	name	1549:1552	arg1	sp					1577:1578	the name Arthrobacter silvisoli sp	1545:1578	the name Arthrobacter silvisoli sp	1545:1578	On the basis of morphological, physiological, biochemical and chemotaxonomic analysis, strain NEAU-SA1T was classified as representing a novel species in the genus Arthrobacter, for which the name Arthrobacter silvisoli sp.
30351271	4	36	theme	16S	438:440	arg1	sequences					452:460	16S rRNA gene sequences	438:460	16S rRNA gene sequences	438:460	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NEAU-SA1T belonged to the genus Arthrobacter and was closely related to Arthrobacter cupressi DSM 24664T (98.1 % similarity).
30351271	13	37	theme	novel	1494:1498	arg1	species					1500:1506	a novel species	1492:1506	a novel species	1492:1506	On the basis of morphological, physiological, biochemical and chemotaxonomic analysis, strain NEAU-SA1T was classified as representing a novel species in the genus Arthrobacter, for which the name Arthrobacter silvisoli sp.
30351271	2	38	theme	life	158:161	arg1	cycle					163:167	a rod-coccus growth life cycle	138:167	a rod-coccus growth life cycle	138:167	A novel Gram-stain-positive, strictly aerobic strain, NEAU-SA1T, which showed a rod-coccus growth life cycle, was isolated from forest soil from Zhangjiajie, Hunan Province, China.
30351271	3	39	theme	%	350:350	arg1	NaCl					358:361	up to 5 % (w/v) NaCl	342:361	up to 5 % (w/v) NaCl	342:361	The isolate grew at 10-40 °C (optimum 28 °C), at pH 5.0-10.0 (optimum pH 7.0) and in the presence of up to 5 % (w/v) NaCl, although NaCl was not required for growth.
30351271	13	40	theme	Arthrobacter	1554:1565	arg1	sp					1577:1578	the name Arthrobacter silvisoli sp	1545:1578	the name Arthrobacter silvisoli sp	1545:1578	On the basis of morphological, physiological, biochemical and chemotaxonomic analysis, strain NEAU-SA1T was classified as representing a novel species in the genus Arthrobacter, for which the name Arthrobacter silvisoli sp.
30351271	15	41	theme	AB	1648:1649	arg1	2017271T					1651:1658	=DSM 106716T=CCTCC AB 2017271T	1629:1658	=DSM 106716T=CCTCC AB 2017271T	1629:1658	The type strain is NEAU-SA1T (=DSM 106716T=CCTCC AB 2017271T).
30351271	15	41	theme	AB	1648:1649	arg1	NEAU-SA1T					1618:1626	NEAU-SA1T	1618:1626	NEAU-SA1T (=DSM 106716T=CCTCC AB 2017271T)	1618:1659	The type strain is NEAU-SA1T (=DSM 106716T=CCTCC AB 2017271T).
30351271	7	42	theme	genomic	913:919	arg1	content					929:935	The genomic DNA G+C content	909:935	The genomic DNA G+C content	909:935	The genomic DNA G+C content was 66.74 mol%.
30351271	7	42	theme	genomic	913:919	arg1	%					950:950	66.74 mol%	941:950	66.74 mol%	941:950	The genomic DNA G+C content was 66.74 mol%.
30351271	2	43	theme	growth	151:156	arg1	cycle					163:167	a rod-coccus growth life cycle	138:167	a rod-coccus growth life cycle	138:167	A novel Gram-stain-positive, strictly aerobic strain, NEAU-SA1T, which showed a rod-coccus growth life cycle, was isolated from forest soil from Zhangjiajie, Hunan Province, China.
30351271	13	44	from	species	1500:1506	arg1	Arthrobacter					1521:1532	the genus Arthrobacter	1511:1532	the genus Arthrobacter	1511:1532	On the basis of morphological, physiological, biochemical and chemotaxonomic analysis, strain NEAU-SA1T was classified as representing a novel species in the genus Arthrobacter, for which the name Arthrobacter silvisoli sp.
30351271	4	45	theme	Phylogenetic	407:418	arg1	analysis					420:427	Phylogenetic analysis	407:427	Phylogenetic analysis based on 16S rRNA gene sequences	407:460	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NEAU-SA1T belonged to the genus Arthrobacter and was closely related to Arthrobacter cupressi DSM 24664T (98.1 % similarity).
30351271	13	46	theme	silvisoli	1567:1575	arg1	sp					1577:1578	the name Arthrobacter silvisoli sp	1545:1578	the name Arthrobacter silvisoli sp	1545:1578	On the basis of morphological, physiological, biochemical and chemotaxonomic analysis, strain NEAU-SA1T was classified as representing a novel species in the genus Arthrobacter, for which the name Arthrobacter silvisoli sp.
30351271	2	47	theme	rod-coccus	140:149	arg1	cycle					163:167	a rod-coccus growth life cycle	138:167	a rod-coccus growth life cycle	138:167	A novel Gram-stain-positive, strictly aerobic strain, NEAU-SA1T, which showed a rod-coccus growth life cycle, was isolated from forest soil from Zhangjiajie, Hunan Province, China.
30351271	13	48	theme	morphological	1373:1385	arg1	analysis					1434:1441	morphological, physiological, biochemical and chemotaxonomic analysis	1373:1441	morphological, physiological, biochemical and chemotaxonomic analysis	1373:1441	On the basis of morphological, physiological, biochemical and chemotaxonomic analysis, strain NEAU-SA1T was classified as representing a novel species in the genus Arthrobacter, for which the name Arthrobacter silvisoli sp.
30351271	10	49	theme	predominant	1174:1184	arg1	MK-9					1202:1205	MK-9	1202:1205	MK-9	1202:1205	The predominant menaquinone was MK-9(H2).
30351271	10	49	theme	predominant	1174:1184	arg1	menaquinone					1186:1196	The predominant menaquinone	1170:1196	The predominant menaquinone	1170:1196	The predominant menaquinone was MK-9(H2).
30351271	4	50	theme	98.1 	590:594	arg1	%					595:595	%	595:595	%	595:595	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NEAU-SA1T belonged to the genus Arthrobacter and was closely related to Arthrobacter cupressi DSM 24664T (98.1 % similarity).
30351271	5	51	theme	DSM	679:681	arg1	24664T					683:688	DSM 24664T	679:688	DSM 24664T	679:688	Average nucleotide identity values between NEAU-SA1T and A. cupressi DSM 24664T were 88.91 and 87.41 % by ANIm and ANIb analysis, respectively.
30351271	2	52	theme	Gram-stain-positive	68:86	arg1	NEAU-SA1T					114:122	NEAU-SA1T	114:122	NEAU-SA1T	114:122	A novel Gram-stain-positive, strictly aerobic strain, NEAU-SA1T, which showed a rod-coccus growth life cycle, was isolated from forest soil from Zhangjiajie, Hunan Province, China.
30351271	2	52	theme	Gram-stain-positive	68:86	arg1	strain					106:111	A novel Gram-stain-positive, strictly aerobic strain	60:111	A novel Gram-stain-positive, strictly aerobic strain	60:111	A novel Gram-stain-positive, strictly aerobic strain, NEAU-SA1T, which showed a rod-coccus growth life cycle, was isolated from forest soil from Zhangjiajie, Hunan Province, China.
30351271	9	53	theme	polar	1051:1055	arg1	lipids					1057:1062	The polar lipids	1047:1062	The polar lipids	1047:1062	The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and two unidentified glycolipids.
30351271	9	53	theme	polar	1051:1055	arg1	diphosphatidylglycerol					1069:1090	diphosphatidylglycerol	1069:1090	diphosphatidylglycerol	1069:1090	The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and two unidentified glycolipids.
30351271	3	54	dep	5 	348:349	arg1	to					345:346	to	345:346	to	345:346	The isolate grew at 10-40 °C (optimum 28 °C), at pH 5.0-10.0 (optimum pH 7.0) and in the presence of up to 5 % (w/v) NaCl, although NaCl was not required for growth.
30351271	12	55	theme	whole-cell	1338:1347	arg1	ribose					1308:1313	ribose	1308:1313	ribose	1308:1313	Glucose, ribose and galactose were the whole-cell sugars.
30351271	12	55	theme	whole-cell	1338:1347	arg1	sugars					1349:1354	the whole-cell sugars	1334:1354	the whole-cell sugars	1334:1354	Glucose, ribose and galactose were the whole-cell sugars.
30351271	12	55	theme	whole-cell	1338:1347	arg1	Glucose					1299:1305	Glucose	1299:1305	Glucose	1299:1305	Glucose, ribose and galactose were the whole-cell sugars.
30351271	12	55	theme	whole-cell	1338:1347	arg1	galactose					1319:1327	galactose	1319:1327	galactose	1319:1327	Glucose, ribose and galactose were the whole-cell sugars.
30351271	2	56	theme	novel	62:66	arg1	NEAU-SA1T					114:122	NEAU-SA1T	114:122	NEAU-SA1T	114:122	A novel Gram-stain-positive, strictly aerobic strain, NEAU-SA1T, which showed a rod-coccus growth life cycle, was isolated from forest soil from Zhangjiajie, Hunan Province, China.
30351271	2	56	theme	novel	62:66	arg1	strain					106:111	A novel Gram-stain-positive, strictly aerobic strain	60:111	A novel Gram-stain-positive, strictly aerobic strain	60:111	A novel Gram-stain-positive, strictly aerobic strain, NEAU-SA1T, which showed a rod-coccus growth life cycle, was isolated from forest soil from Zhangjiajie, Hunan Province, China.
30351271	8	57	theme	cellular	963:970	arg1	acids					978:982	The major cellular fatty acids	953:982	The major cellular fatty acids (>10 %)	953:990	The major cellular fatty acids (>10 %) were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
30351271	8	57	theme	cellular	963:970	arg1	%					989:989	>10 %	985:989	>10 %	985:989	The major cellular fatty acids (>10 %) were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
30351271	8	57	theme	cellular	963:970	arg1	anteiso-C15 					997:1008	anteiso-C15 	997:1008	anteiso-C15 	997:1008	The major cellular fatty acids (>10 %) were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
30351271	6	58	dep	A.	825:826	arg1	cupressi					828:835	cupressi	828:835	cupressi	828:835	The in silico DNA-DNA hybridization value between strain NEAU-SA1T and A. cupressi DSM 24664T was 34.20 %, again indicating they belong to different taxa.
30351271	3	59	theme	NaCl	358:361	arg1	presence					330:337	the presence	326:337	the presence of up to 5 % (w/v) NaCl	326:361	The isolate grew at 10-40 °C (optimum 28 °C), at pH 5.0-10.0 (optimum pH 7.0) and in the presence of up to 5 % (w/v) NaCl, although NaCl was not required for growth.
30351271	13	60	theme	biochemical	1403:1413	arg1	analysis					1434:1441	morphological, physiological, biochemical and chemotaxonomic analysis	1373:1441	morphological, physiological, biochemical and chemotaxonomic analysis	1373:1441	On the basis of morphological, physiological, biochemical and chemotaxonomic analysis, strain NEAU-SA1T was classified as representing a novel species in the genus Arthrobacter, for which the name Arthrobacter silvisoli sp.
30351271	8	61	theme	major	957:961	arg1	acids					978:982	The major cellular fatty acids	953:982	The major cellular fatty acids (>10 %)	953:990	The major cellular fatty acids (>10 %) were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
30351271	8	61	theme	major	957:961	arg1	%					989:989	>10 %	985:989	>10 %	985:989	The major cellular fatty acids (>10 %) were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
30351271	8	61	theme	major	957:961	arg1	anteiso-C15 					997:1008	anteiso-C15 	997:1008	anteiso-C15 	997:1008	The major cellular fatty acids (>10 %) were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
30351271	13	62	theme	chemotaxonomic	1419:1432	arg1	analysis					1434:1441	morphological, physiological, biochemical and chemotaxonomic analysis	1373:1441	morphological, physiological, biochemical and chemotaxonomic analysis	1373:1441	On the basis of morphological, physiological, biochemical and chemotaxonomic analysis, strain NEAU-SA1T was classified as representing a novel species in the genus Arthrobacter, for which the name Arthrobacter silvisoli sp.
30351271	4	63	theme	%	595:595	arg1	DSM					578:580	Arthrobacter cupressi DSM 24664T	556:587	Arthrobacter cupressi DSM 24664T (98.1 % similarity)	556:607	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NEAU-SA1T belonged to the genus Arthrobacter and was closely related to Arthrobacter cupressi DSM 24664T (98.1 % similarity).
30351271	4	63	theme	%	595:595	arg1	similarity					597:606	98.1 % similarity	590:606	98.1 % similarity	590:606	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NEAU-SA1T belonged to the genus Arthrobacter and was closely related to Arthrobacter cupressi DSM 24664T (98.1 % similarity).
30351271	4	64	theme	strain	477:482	arg1	NEAU-SA1T					484:492	strain NEAU-SA1T	477:492	strain NEAU-SA1T	477:492	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NEAU-SA1T belonged to the genus Arthrobacter and was closely related to Arthrobacter cupressi DSM 24664T (98.1 % similarity).
30351271	5	65	theme	identity	629:636	arg1	values					638:643	Average nucleotide identity values	610:643	Average nucleotide identity values between NEAU-SA1T and A. cupressi DSM 24664T	610:688	Average nucleotide identity values between NEAU-SA1T and A. cupressi DSM 24664T were 88.91 and 87.41 % by ANIm and ANIb analysis, respectively.
30351271	4	66	theme	cupressi	569:576	arg1	DSM					578:580	Arthrobacter cupressi DSM 24664T	556:587	Arthrobacter cupressi DSM 24664T (98.1 % similarity)	556:607	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NEAU-SA1T belonged to the genus Arthrobacter and was closely related to Arthrobacter cupressi DSM 24664T (98.1 % similarity).
30351271	4	66	theme	cupressi	569:576	arg1	similarity					597:606	98.1 % similarity	590:606	98.1 % similarity	590:606	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NEAU-SA1T belonged to the genus Arthrobacter and was closely related to Arthrobacter cupressi DSM 24664T (98.1 % similarity).
30351271	6	67	dep	in	758:759	arg1	silico					761:766	silico	761:766	silico	761:766	The in silico DNA-DNA hybridization value between strain NEAU-SA1T and A. cupressi DSM 24664T was 34.20 %, again indicating they belong to different taxa.
30351271	8	68	theme	fatty	972:976	arg1	acids					978:982	The major cellular fatty acids	953:982	The major cellular fatty acids (>10 %)	953:990	The major cellular fatty acids (>10 %) were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
30351271	8	68	theme	fatty	972:976	arg1	%					989:989	>10 %	985:989	>10 %	985:989	The major cellular fatty acids (>10 %) were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
30351271	8	68	theme	fatty	972:976	arg1	anteiso-C15 					997:1008	anteiso-C15 	997:1008	anteiso-C15 	997:1008	The major cellular fatty acids (>10 %) were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
30351271	2	69	dep	Gram-stain-positive	68:86	arg1	aerobic					98:104	aerobic	98:104	aerobic	98:104	A novel Gram-stain-positive, strictly aerobic strain, NEAU-SA1T, which showed a rod-coccus growth life cycle, was isolated from forest soil from Zhangjiajie, Hunan Province, China.
30351271	4	70	theme	Arthrobacter	556:567	arg1	DSM					578:580	Arthrobacter cupressi DSM 24664T	556:587	Arthrobacter cupressi DSM 24664T (98.1 % similarity)	556:607	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NEAU-SA1T belonged to the genus Arthrobacter and was closely related to Arthrobacter cupressi DSM 24664T (98.1 % similarity).
30351271	4	70	theme	Arthrobacter	556:567	arg1	similarity					597:606	98.1 % similarity	590:606	98.1 % similarity	590:606	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NEAU-SA1T belonged to the genus Arthrobacter and was closely related to Arthrobacter cupressi DSM 24664T (98.1 % similarity).
30010529	9	0	theme	peptidoglycan	1051:1063	arg1	acid					1089:1092	meso-diaminopimelic acid	1069:1092	meso-diaminopimelic acid	1069:1092	The diagnostic diamino acid of the peptidoglycan was meso-diaminopimelic acid.
30010529	9	0	theme	peptidoglycan	1051:1063	arg1	acid					1039:1042	The diagnostic diamino acid	1016:1042	The diagnostic diamino acid of the peptidoglycan	1016:1063	The diagnostic diamino acid of the peptidoglycan was meso-diaminopimelic acid.
30010529	9	1	theme	diagnostic	1020:1029	arg1	acid					1089:1092	meso-diaminopimelic acid	1069:1092	meso-diaminopimelic acid	1069:1092	The diagnostic diamino acid of the peptidoglycan was meso-diaminopimelic acid.
30010529	9	1	theme	diagnostic	1020:1029	arg1	acid					1039:1042	The diagnostic diamino acid	1016:1042	The diagnostic diamino acid of the peptidoglycan	1016:1063	The diagnostic diamino acid of the peptidoglycan was meso-diaminopimelic acid.
30010529	5	2	theme	Sphaerisporangium	611:627	arg1	NEAU-CY18T					638:647	Sphaerisporangium dianthi' NEAU-CY18T	611:647	Sphaerisporangium dianthi' NEAU-CY18T (99.2 % 16S rRNA gene sequence similarity)	611:690	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Sphaerisporangium and that it forms a monophyletic clade with its closest relatives 'Sphaerisporangium dianthi' NEAU-CY18T (99.2 % 16S rRNA gene sequence similarity) and Sphaerisporangium cinnabarinum JCM 3291T (98.8 %).
30010529	5	2	theme	Sphaerisporangium	611:627	arg1	relatives					600:608	its closest relatives	588:608	its closest relatives 'Sphaerisporangium dianthi' NEAU-CY18T (99.2 % 16S rRNA gene sequence similarity) and Sphaerisporangium cinnabarinum JCM 3291T (98.8 %)	588:744	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Sphaerisporangium and that it forms a monophyletic clade with its closest relatives 'Sphaerisporangium dianthi' NEAU-CY18T (99.2 % 16S rRNA gene sequence similarity) and Sphaerisporangium cinnabarinum JCM 3291T (98.8 %).
30010529	5	2	theme	Sphaerisporangium	611:627	arg1	similarity					680:689	99.2 % 16S rRNA gene sequence similarity	650:689	99.2 % 16S rRNA gene sequence similarity	650:689	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Sphaerisporangium and that it forms a monophyletic clade with its closest relatives 'Sphaerisporangium dianthi' NEAU-CY18T (99.2 % 16S rRNA gene sequence similarity) and Sphaerisporangium cinnabarinum JCM 3291T (98.8 %).
30010529	3	3	theme	novel	145:149	arg1	actinomycete					151:162	A novel actinomycete	143:162	A novel actinomycete	143:162	A novel actinomycete, designated strain NEAU-mq3T, was isolated from the rhizosphere soil of a rubber tree (Hevea brasiliensis Muell.
30010529	13	4	theme	name	1783:1786	arg1	sp					1820:1821	the name Sphaerisporangium rhizosphaerae sp	1779:1821	the name Sphaerisporangium rhizosphaerae sp	1779:1821	Therefore, it is concluded that strain NEAU-mq3T represents a novel species of the genus Sphaerisporangium, for which the name Sphaerisporangium rhizosphaerae sp.
30010529	3	5	attach	isolated	198:205	arg1	soil					228:231	the rhizosphere soil	212:231	the rhizosphere soil of a rubber tree	212:248	A novel actinomycete, designated strain NEAU-mq3T, was isolated from the rhizosphere soil of a rubber tree (Hevea brasiliensis Muell.
30010529	3	5	attach	isolated	198:205	arg2	actinomycete					151:162	A novel actinomycete	143:162	A novel actinomycete	143:162	A novel actinomycete, designated strain NEAU-mq3T, was isolated from the rhizosphere soil of a rubber tree (Hevea brasiliensis Muell.
30010529	11	6	theme	10-methyl	1363:1371	arg1	 0					1359:1360	 0	1359:1360	 0	1359:1360	The major fatty acids were identified as iso-C16 : 0, 10-methyl C17 : 0, C16 : 1ω7c and C17 : 1ω7c.
30010529	11	6	theme	10-methyl	1363:1371	arg1	C17 					1373:1376	10-methyl C17 	1363:1376	10-methyl C17 	1363:1376	The major fatty acids were identified as iso-C16 : 0, 10-methyl C17 : 0, C16 : 1ω7c and C17 : 1ω7c.
30010529	6	7	theme	Sphaerisporangium	866:882	arg1	description					841:851	the description	837:851	the description of the genus Sphaerisporangium	837:882	Morphological and chemotaxonomic properties of strain NEAU-mq3T were also consistent with the description of the genus Sphaerisporangium.
30010529	6	8	theme	chemotaxonomic	765:778	arg1	properties					780:789	Morphological and chemotaxonomic properties	747:789	Morphological and chemotaxonomic properties of strain NEAU-mq3T	747:809	Morphological and chemotaxonomic properties of strain NEAU-mq3T were also consistent with the description of the genus Sphaerisporangium.
30010529	10	9	theme	phospholipid	1099:1110	arg1	profile					1112:1118	The phospholipid profile	1095:1118	The phospholipid profile	1095:1118	The phospholipid profile consisted of diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol mannoside, an unidentified polar lipid and an unidentified phospholipid.
30010529	6	10	theme	genus	860:864	arg1	Sphaerisporangium					866:882	the genus Sphaerisporangium	856:882	the genus Sphaerisporangium	856:882	Morphological and chemotaxonomic properties of strain NEAU-mq3T were also consistent with the description of the genus Sphaerisporangium.
30010529	5	11	theme	monophyletic	564:575	arg1	clade					577:581	a monophyletic clade	562:581	a monophyletic clade with its closest relatives 'Sphaerisporangium dianthi' NEAU-CY18T (99.2 % 16S rRNA gene sequence similarity) and Sphaerisporangium cinnabarinum JCM 3291T (98.8 %)	562:744	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Sphaerisporangium and that it forms a monophyletic clade with its closest relatives 'Sphaerisporangium dianthi' NEAU-CY18T (99.2 % 16S rRNA gene sequence similarity) and Sphaerisporangium cinnabarinum JCM 3291T (98.8 %).
30010529	1	12	dep	brasiliensis	117:128	arg1	Muell					130:134	Muell	130:134	Muell	130:134	nov., an actinomycete isolated from the rhizosphere soil of a rubber tree (Hevea brasiliensis Muell.
30010529	5	13	theme	gene	452:455	arg1	sequence					457:464	the 16S rRNA gene sequence	439:464	the 16S rRNA gene sequence	439:464	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Sphaerisporangium and that it forms a monophyletic clade with its closest relatives 'Sphaerisporangium dianthi' NEAU-CY18T (99.2 % 16S rRNA gene sequence similarity) and Sphaerisporangium cinnabarinum JCM 3291T (98.8 %).
30010529	10	14	theme	unidentified	1250:1261	arg1	lipid					1269:1273	an unidentified polar lipid	1247:1273	an unidentified polar lipid	1247:1273	The phospholipid profile consisted of diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol mannoside, an unidentified polar lipid and an unidentified phospholipid.
30010529	13	15	theme	Sphaerisporangium	1788:1804	arg1	sp					1820:1821	the name Sphaerisporangium rhizosphaerae sp	1779:1821	the name Sphaerisporangium rhizosphaerae sp	1779:1821	Therefore, it is concluded that strain NEAU-mq3T represents a novel species of the genus Sphaerisporangium, for which the name Sphaerisporangium rhizosphaerae sp.
30010529	6	16	theme	Morphological	747:759	arg1	properties					780:789	Morphological and chemotaxonomic properties	747:789	Morphological and chemotaxonomic properties of strain NEAU-mq3T	747:809	Morphological and chemotaxonomic properties of strain NEAU-mq3T were also consistent with the description of the genus Sphaerisporangium.
30010529	6	17	with	consistent	821:830	arg1	description					841:851	the description	837:851	the description of the genus Sphaerisporangium	837:882	Morphological and chemotaxonomic properties of strain NEAU-mq3T were also consistent with the description of the genus Sphaerisporangium.
30010529	5	18	theme	Sphaerisporangium	696:712	arg1	relatives					600:608	its closest relatives	588:608	its closest relatives 'Sphaerisporangium dianthi' NEAU-CY18T (99.2 % 16S rRNA gene sequence similarity) and Sphaerisporangium cinnabarinum JCM 3291T (98.8 %)	588:744	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Sphaerisporangium and that it forms a monophyletic clade with its closest relatives 'Sphaerisporangium dianthi' NEAU-CY18T (99.2 % 16S rRNA gene sequence similarity) and Sphaerisporangium cinnabarinum JCM 3291T (98.8 %).
30010529	5	18	theme	Sphaerisporangium	696:712	arg1	3291T					731:735	Sphaerisporangium cinnabarinum JCM 3291T	696:735	Sphaerisporangium cinnabarinum JCM 3291T (98.8 %)	696:744	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Sphaerisporangium and that it forms a monophyletic clade with its closest relatives 'Sphaerisporangium dianthi' NEAU-CY18T (99.2 % 16S rRNA gene sequence similarity) and Sphaerisporangium cinnabarinum JCM 3291T (98.8 %).
30010529	5	18	theme	Sphaerisporangium	696:712	arg1	%					743:743	98.8 %	738:743	98.8 %	738:743	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Sphaerisporangium and that it forms a monophyletic clade with its closest relatives 'Sphaerisporangium dianthi' NEAU-CY18T (99.2 % 16S rRNA gene sequence similarity) and Sphaerisporangium cinnabarinum JCM 3291T (98.8 %).
30010529	11	19	theme	fatty	1319:1323	arg1	iso-C16 					1350:1357	iso-C16 	1350:1357	iso-C16 	1350:1357	The major fatty acids were identified as iso-C16 : 0, 10-methyl C17 : 0, C16 : 1ω7c and C17 : 1ω7c.
30010529	11	19	theme	fatty	1319:1323	arg1	acids					1325:1329	The major fatty acids	1309:1329	The major fatty acids	1309:1329	The major fatty acids were identified as iso-C16 : 0, 10-methyl C17 : 0, C16 : 1ω7c and C17 : 1ω7c.
30010529	15	20	theme	=CGMCC	1872:1877	arg1	32389T					1891:1896	=CGMCC 4.7429T=JCM 32389T	1872:1896	=CGMCC 4.7429T=JCM 32389T	1872:1896	The type strain is NEAU-mq3T (=CGMCC 4.7429T=JCM 32389T).
30010529	15	20	theme	=CGMCC	1872:1877	arg1	NEAU-mq3T					1861:1869	NEAU-mq3T	1861:1869	NEAU-mq3T (=CGMCC 4.7429T=JCM 32389T)	1861:1897	The type strain is NEAU-mq3T (=CGMCC 4.7429T=JCM 32389T).
30010529	3	21	theme	strain	176:181	arg1	NEAU-mq3T					183:191	strain NEAU-mq3T	176:191	strain NEAU-mq3T	176:191	A novel actinomycete, designated strain NEAU-mq3T, was isolated from the rhizosphere soil of a rubber tree (Hevea brasiliensis Muell.
30010529	1	22	theme	rhizosphere	76:86	arg1	soil					88:91	the rhizosphere soil	72:91	the rhizosphere soil of a rubber tree (Hevea brasiliensis Muell	72:134	nov., an actinomycete isolated from the rhizosphere soil of a rubber tree (Hevea brasiliensis Muell.
30010529	0	23	theme	rhizosphaerae	18:30	arg1	sp					32:33	Sphaerisporangium rhizosphaerae sp	0:33	Sphaerisporangium rhizosphaerae sp.	0:34	Sphaerisporangium rhizosphaerae sp.
30010529	5	24	theme	closest	592:598	arg1	NEAU-CY18T					638:647	Sphaerisporangium dianthi' NEAU-CY18T	611:647	Sphaerisporangium dianthi' NEAU-CY18T (99.2 % 16S rRNA gene sequence similarity)	611:690	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Sphaerisporangium and that it forms a monophyletic clade with its closest relatives 'Sphaerisporangium dianthi' NEAU-CY18T (99.2 % 16S rRNA gene sequence similarity) and Sphaerisporangium cinnabarinum JCM 3291T (98.8 %).
30010529	5	24	theme	closest	592:598	arg1	relatives					600:608	its closest relatives	588:608	its closest relatives 'Sphaerisporangium dianthi' NEAU-CY18T (99.2 % 16S rRNA gene sequence similarity) and Sphaerisporangium cinnabarinum JCM 3291T (98.8 %)	588:744	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Sphaerisporangium and that it forms a monophyletic clade with its closest relatives 'Sphaerisporangium dianthi' NEAU-CY18T (99.2 % 16S rRNA gene sequence similarity) and Sphaerisporangium cinnabarinum JCM 3291T (98.8 %).
30010529	5	24	theme	closest	592:598	arg1	3291T					731:735	Sphaerisporangium cinnabarinum JCM 3291T	696:735	Sphaerisporangium cinnabarinum JCM 3291T (98.8 %)	696:744	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Sphaerisporangium and that it forms a monophyletic clade with its closest relatives 'Sphaerisporangium dianthi' NEAU-CY18T (99.2 % 16S rRNA gene sequence similarity) and Sphaerisporangium cinnabarinum JCM 3291T (98.8 %).
30010529	5	25	dep	Sphaerisporangium	696:712	arg1	cinnabarinum					714:725	cinnabarinum	714:725	cinnabarinum	714:725	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Sphaerisporangium and that it forms a monophyletic clade with its closest relatives 'Sphaerisporangium dianthi' NEAU-CY18T (99.2 % 16S rRNA gene sequence similarity) and Sphaerisporangium cinnabarinum JCM 3291T (98.8 %).
30010529	0	26	theme	Sphaerisporangium	0:16	arg1	sp					32:33	Sphaerisporangium rhizosphaerae sp	0:33	Sphaerisporangium rhizosphaerae sp.	0:34	Sphaerisporangium rhizosphaerae sp.
30010529	3	27	theme	rhizosphere	216:226	arg1	soil					228:231	the rhizosphere soil	212:231	the rhizosphere soil of a rubber tree	212:248	A novel actinomycete, designated strain NEAU-mq3T, was isolated from the rhizosphere soil of a rubber tree (Hevea brasiliensis Muell.
30010529	15	28	theme	4.7429T=JCM	1879:1889	arg1	32389T					1891:1896	=CGMCC 4.7429T=JCM 32389T	1872:1896	=CGMCC 4.7429T=JCM 32389T	1872:1896	The type strain is NEAU-mq3T (=CGMCC 4.7429T=JCM 32389T).
30010529	15	28	theme	4.7429T=JCM	1879:1889	arg1	NEAU-mq3T					1861:1869	NEAU-mq3T	1861:1869	NEAU-mq3T (=CGMCC 4.7429T=JCM 32389T)	1861:1897	The type strain is NEAU-mq3T (=CGMCC 4.7429T=JCM 32389T).
30010529	3	29	dep	isolated	198:205	arg1	Muell					270:274	Muell	270:274	Muell	270:274	A novel actinomycete, designated strain NEAU-mq3T, was isolated from the rhizosphere soil of a rubber tree (Hevea brasiliensis Muell.
30010529	1	30	attach	isolated	58:65	arg2	actinomycete					45:56	an actinomycete	42:56	an actinomycete isolated from the rhizosphere soil of a rubber tree (Hevea brasiliensis Muell	42:134	nov., an actinomycete isolated from the rhizosphere soil of a rubber tree (Hevea brasiliensis Muell.
30010529	1	30	attach	isolated	58:65	arg1	soil					88:91	the rhizosphere soil	72:91	the rhizosphere soil of a rubber tree (Hevea brasiliensis Muell	72:134	nov., an actinomycete isolated from the rhizosphere soil of a rubber tree (Hevea brasiliensis Muell.
30010529	3	31	theme	tree	245:248	arg1	soil					228:231	the rhizosphere soil	212:231	the rhizosphere soil of a rubber tree	212:248	A novel actinomycete, designated strain NEAU-mq3T, was isolated from the rhizosphere soil of a rubber tree (Hevea brasiliensis Muell.
30010529	4	32	theme	north-east	340:349	arg1	Province					330:337	Heilongjiang Province	317:337	Heilongjiang Province	317:337	Arg) collected from Xianglu Mountain in Heilongjiang Province, north-east China, and characterized by using a polyphasic approach.
30010529	4	32	theme	north-east	340:349	arg1	China					351:355	north-east China	340:355	north-east China	340:355	Arg) collected from Xianglu Mountain in Heilongjiang Province, north-east China, and characterized by using a polyphasic approach.
30010529	9	33	theme	meso-diaminopimelic	1069:1087	arg1	acid					1089:1092	meso-diaminopimelic acid	1069:1092	meso-diaminopimelic acid	1069:1092	The diagnostic diamino acid of the peptidoglycan was meso-diaminopimelic acid.
30010529	9	33	theme	meso-diaminopimelic	1069:1087	arg1	acid					1039:1042	The diagnostic diamino acid	1016:1042	The diagnostic diamino acid of the peptidoglycan	1016:1063	The diagnostic diamino acid of the peptidoglycan was meso-diaminopimelic acid.
30010529	10	34	theme	unidentified	1282:1293	arg1	phospholipid					1295:1306	an unidentified phospholipid	1279:1306	an unidentified phospholipid	1279:1306	The phospholipid profile consisted of diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol mannoside, an unidentified polar lipid and an unidentified phospholipid.
30010529	5	35	theme	rRNA	661:664	arg1	similarity					680:689	99.2 % 16S rRNA gene sequence similarity	650:689	99.2 % 16S rRNA gene sequence similarity	650:689	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Sphaerisporangium and that it forms a monophyletic clade with its closest relatives 'Sphaerisporangium dianthi' NEAU-CY18T (99.2 % 16S rRNA gene sequence similarity) and Sphaerisporangium cinnabarinum JCM 3291T (98.8 %).
30010529	5	35	theme	rRNA	661:664	arg1	NEAU-CY18T					638:647	Sphaerisporangium dianthi' NEAU-CY18T	611:647	Sphaerisporangium dianthi' NEAU-CY18T (99.2 % 16S rRNA gene sequence similarity)	611:690	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Sphaerisporangium and that it forms a monophyletic clade with its closest relatives 'Sphaerisporangium dianthi' NEAU-CY18T (99.2 % 16S rRNA gene sequence similarity) and Sphaerisporangium cinnabarinum JCM 3291T (98.8 %).
30010529	11	36	dep	 0	1359:1360	arg1	 1ω7c					1387:1391	 1ω7c	1387:1391	 1ω7c	1387:1391	The major fatty acids were identified as iso-C16 : 0, 10-methyl C17 : 0, C16 : 1ω7c and C17 : 1ω7c.
30010529	11	36	dep	 0	1359:1360	arg1	C16 					1382:1385	C16 	1382:1385	C16 	1382:1385	The major fatty acids were identified as iso-C16 : 0, 10-methyl C17 : 0, C16 : 1ω7c and C17 : 1ω7c.
30010529	11	36	dep	 0	1359:1360	arg1	 1ω7c					1402:1406	 1ω7c	1402:1406	 1ω7c	1402:1406	The major fatty acids were identified as iso-C16 : 0, 10-methyl C17 : 0, C16 : 1ω7c and C17 : 1ω7c.
30010529	11	36	dep	 0	1359:1360	arg1	C17 					1397:1400	C17 	1397:1400	C17 	1397:1400	The major fatty acids were identified as iso-C16 : 0, 10-methyl C17 : 0, C16 : 1ω7c and C17 : 1ω7c.
30010529	1	37	theme	rubber	98:103	arg1	brasiliensis					117:128	a rubber tree (Hevea brasiliensis Muell	96:134	a rubber tree (Hevea brasiliensis Muell	96:134	nov., an actinomycete isolated from the rhizosphere soil of a rubber tree (Hevea brasiliensis Muell.
30010529	5	38	theme	16S	443:445	arg1	rRNA					447:450	the 16S rRNA	439:450	the 16S rRNA gene sequence	439:464	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Sphaerisporangium and that it forms a monophyletic clade with its closest relatives 'Sphaerisporangium dianthi' NEAU-CY18T (99.2 % 16S rRNA gene sequence similarity) and Sphaerisporangium cinnabarinum JCM 3291T (98.8 %).
30010529	1	39	dep	actinomycete	45:56	arg1	nov.					36:39	nov.	36:39	nov.	36:39	nov., an actinomycete isolated from the rhizosphere soil of a rubber tree (Hevea brasiliensis Muell.
30010529	12	40	theme	DNA-DNA	1409:1415	arg1	experiments					1431:1441	DNA-DNA hybridization experiments	1409:1441	DNA-DNA hybridization experiments	1409:1441	DNA-DNA hybridization experiments and phenotypic tests were carried out between strain NEAU-mq3T and its most closely related strains, which further clarified their relatedness and demonstrated that NEAU-mq3T could be distinguished from these strains.
30010529	5	41	theme	gene	666:669	arg1	similarity					680:689	99.2 % 16S rRNA gene sequence similarity	650:689	99.2 % 16S rRNA gene sequence similarity	650:689	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Sphaerisporangium and that it forms a monophyletic clade with its closest relatives 'Sphaerisporangium dianthi' NEAU-CY18T (99.2 % 16S rRNA gene sequence similarity) and Sphaerisporangium cinnabarinum JCM 3291T (98.8 %).
30010529	5	41	theme	gene	666:669	arg1	NEAU-CY18T					638:647	Sphaerisporangium dianthi' NEAU-CY18T	611:647	Sphaerisporangium dianthi' NEAU-CY18T (99.2 % 16S rRNA gene sequence similarity)	611:690	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Sphaerisporangium and that it forms a monophyletic clade with its closest relatives 'Sphaerisporangium dianthi' NEAU-CY18T (99.2 % 16S rRNA gene sequence similarity) and Sphaerisporangium cinnabarinum JCM 3291T (98.8 %).
30010529	12	42	theme	hybridization	1417:1429	arg1	experiments					1431:1441	DNA-DNA hybridization experiments	1409:1441	DNA-DNA hybridization experiments	1409:1441	DNA-DNA hybridization experiments and phenotypic tests were carried out between strain NEAU-mq3T and its most closely related strains, which further clarified their relatedness and demonstrated that NEAU-mq3T could be distinguished from these strains.
30010529	5	43	theme	rRNA	447:450	arg1	sequence					457:464	the 16S rRNA gene sequence	439:464	the 16S rRNA gene sequence	439:464	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Sphaerisporangium and that it forms a monophyletic clade with its closest relatives 'Sphaerisporangium dianthi' NEAU-CY18T (99.2 % 16S rRNA gene sequence similarity) and Sphaerisporangium cinnabarinum JCM 3291T (98.8 %).
30010529	9	44	theme	diamino	1031:1037	arg1	acid					1089:1092	meso-diaminopimelic acid	1069:1092	meso-diaminopimelic acid	1069:1092	The diagnostic diamino acid of the peptidoglycan was meso-diaminopimelic acid.
30010529	9	44	theme	diamino	1031:1037	arg1	acid					1039:1042	The diagnostic diamino acid	1016:1042	The diagnostic diamino acid of the peptidoglycan	1016:1063	The diagnostic diamino acid of the peptidoglycan was meso-diaminopimelic acid.
30010529	5	45	dep	relatives	600:608	arg1	NEAU-CY18T					638:647	Sphaerisporangium dianthi' NEAU-CY18T	611:647	Sphaerisporangium dianthi' NEAU-CY18T (99.2 % 16S rRNA gene sequence similarity)	611:690	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Sphaerisporangium and that it forms a monophyletic clade with its closest relatives 'Sphaerisporangium dianthi' NEAU-CY18T (99.2 % 16S rRNA gene sequence similarity) and Sphaerisporangium cinnabarinum JCM 3291T (98.8 %).
30010529	5	45	dep	relatives	600:608	arg1	relatives					600:608	its closest relatives	588:608	its closest relatives 'Sphaerisporangium dianthi' NEAU-CY18T (99.2 % 16S rRNA gene sequence similarity) and Sphaerisporangium cinnabarinum JCM 3291T (98.8 %)	588:744	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Sphaerisporangium and that it forms a monophyletic clade with its closest relatives 'Sphaerisporangium dianthi' NEAU-CY18T (99.2 % 16S rRNA gene sequence similarity) and Sphaerisporangium cinnabarinum JCM 3291T (98.8 %).
30010529	5	45	dep	relatives	600:608	arg1	3291T					731:735	Sphaerisporangium cinnabarinum JCM 3291T	696:735	Sphaerisporangium cinnabarinum JCM 3291T (98.8 %)	696:744	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Sphaerisporangium and that it forms a monophyletic clade with its closest relatives 'Sphaerisporangium dianthi' NEAU-CY18T (99.2 % 16S rRNA gene sequence similarity) and Sphaerisporangium cinnabarinum JCM 3291T (98.8 %).
30010529	5	45	dep	relatives	600:608	arg1	%					743:743	98.8 %	738:743	98.8 %	738:743	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Sphaerisporangium and that it forms a monophyletic clade with its closest relatives 'Sphaerisporangium dianthi' NEAU-CY18T (99.2 % 16S rRNA gene sequence similarity) and Sphaerisporangium cinnabarinum JCM 3291T (98.8 %).
30010529	5	45	dep	relatives	600:608	arg1	similarity					680:689	99.2 % 16S rRNA gene sequence similarity	650:689	99.2 % 16S rRNA gene sequence similarity	650:689	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Sphaerisporangium and that it forms a monophyletic clade with its closest relatives 'Sphaerisporangium dianthi' NEAU-CY18T (99.2 % 16S rRNA gene sequence similarity) and Sphaerisporangium cinnabarinum JCM 3291T (98.8 %).
30010529	3	46	theme	rubber	238:243	arg1	tree					245:248	a rubber tree	236:248	a rubber tree	236:248	A novel actinomycete, designated strain NEAU-mq3T, was isolated from the rhizosphere soil of a rubber tree (Hevea brasiliensis Muell.
30010529	12	47	theme	phenotypic	1447:1456	arg1	tests					1458:1462	phenotypic tests	1447:1462	phenotypic tests	1447:1462	DNA-DNA hybridization experiments and phenotypic tests were carried out between strain NEAU-mq3T and its most closely related strains, which further clarified their relatedness and demonstrated that NEAU-mq3T could be distinguished from these strains.
30010529	15	48	theme	type	1846:1849	arg1	strain					1851:1856	The type strain	1842:1856	The type strain	1842:1856	The type strain is NEAU-mq3T (=CGMCC 4.7429T=JCM 32389T).
30010529	15	48	theme	type	1846:1849	arg1	NEAU-mq3T					1861:1869	NEAU-mq3T	1861:1869	NEAU-mq3T (=CGMCC 4.7429T=JCM 32389T)	1861:1897	The type strain is NEAU-mq3T (=CGMCC 4.7429T=JCM 32389T).
30010529	11	49	theme	major	1313:1317	arg1	iso-C16 					1350:1357	iso-C16 	1350:1357	iso-C16 	1350:1357	The major fatty acids were identified as iso-C16 : 0, 10-methyl C17 : 0, C16 : 1ω7c and C17 : 1ω7c.
30010529	11	49	theme	major	1313:1317	arg1	acids					1325:1329	The major fatty acids	1309:1329	The major fatty acids	1309:1329	The major fatty acids were identified as iso-C16 : 0, 10-methyl C17 : 0, C16 : 1ω7c and C17 : 1ω7c.
30010529	5	50	with	clade	577:581	arg1	NEAU-CY18T					638:647	Sphaerisporangium dianthi' NEAU-CY18T	611:647	Sphaerisporangium dianthi' NEAU-CY18T (99.2 % 16S rRNA gene sequence similarity)	611:690	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Sphaerisporangium and that it forms a monophyletic clade with its closest relatives 'Sphaerisporangium dianthi' NEAU-CY18T (99.2 % 16S rRNA gene sequence similarity) and Sphaerisporangium cinnabarinum JCM 3291T (98.8 %).
30010529	5	50	with	clade	577:581	arg1	relatives					600:608	its closest relatives	588:608	its closest relatives 'Sphaerisporangium dianthi' NEAU-CY18T (99.2 % 16S rRNA gene sequence similarity) and Sphaerisporangium cinnabarinum JCM 3291T (98.8 %)	588:744	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Sphaerisporangium and that it forms a monophyletic clade with its closest relatives 'Sphaerisporangium dianthi' NEAU-CY18T (99.2 % 16S rRNA gene sequence similarity) and Sphaerisporangium cinnabarinum JCM 3291T (98.8 %).
30010529	5	50	with	clade	577:581	arg1	3291T					731:735	Sphaerisporangium cinnabarinum JCM 3291T	696:735	Sphaerisporangium cinnabarinum JCM 3291T (98.8 %)	696:744	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Sphaerisporangium and that it forms a monophyletic clade with its closest relatives 'Sphaerisporangium dianthi' NEAU-CY18T (99.2 % 16S rRNA gene sequence similarity) and Sphaerisporangium cinnabarinum JCM 3291T (98.8 %).
30010529	10	51	theme	polar	1263:1267	arg1	lipid					1269:1273	an unidentified polar lipid	1247:1273	an unidentified polar lipid	1247:1273	The phospholipid profile consisted of diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol mannoside, an unidentified polar lipid and an unidentified phospholipid.
30010529	13	52	theme	Sphaerisporangium	1750:1766	arg1	species					1729:1735	a novel species	1721:1735	a novel species	1721:1735	Therefore, it is concluded that strain NEAU-mq3T represents a novel species of the genus Sphaerisporangium, for which the name Sphaerisporangium rhizosphaerae sp.
30010529	1	53	theme	tree	105:108	arg1	brasiliensis					117:128	a rubber tree (Hevea brasiliensis Muell	96:134	a rubber tree (Hevea brasiliensis Muell	96:134	nov., an actinomycete isolated from the rhizosphere soil of a rubber tree (Hevea brasiliensis Muell.
30010529	5	54	theme	sequence	671:678	arg1	similarity					680:689	99.2 % 16S rRNA gene sequence similarity	650:689	99.2 % 16S rRNA gene sequence similarity	650:689	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Sphaerisporangium and that it forms a monophyletic clade with its closest relatives 'Sphaerisporangium dianthi' NEAU-CY18T (99.2 % 16S rRNA gene sequence similarity) and Sphaerisporangium cinnabarinum JCM 3291T (98.8 %).
30010529	5	54	theme	sequence	671:678	arg1	NEAU-CY18T					638:647	Sphaerisporangium dianthi' NEAU-CY18T	611:647	Sphaerisporangium dianthi' NEAU-CY18T (99.2 % 16S rRNA gene sequence similarity)	611:690	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Sphaerisporangium and that it forms a monophyletic clade with its closest relatives 'Sphaerisporangium dianthi' NEAU-CY18T (99.2 % 16S rRNA gene sequence similarity) and Sphaerisporangium cinnabarinum JCM 3291T (98.8 %).
30010529	12	55	theme	related	1527:1533	arg1	strains					1535:1541	its most closely related strains	1510:1541	its most closely related strains	1510:1541	DNA-DNA hybridization experiments and phenotypic tests were carried out between strain NEAU-mq3T and its most closely related strains, which further clarified their relatedness and demonstrated that NEAU-mq3T could be distinguished from these strains.
30010529	5	56	theme	16S	657:659	arg1	similarity					680:689	99.2 % 16S rRNA gene sequence similarity	650:689	99.2 % 16S rRNA gene sequence similarity	650:689	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Sphaerisporangium and that it forms a monophyletic clade with its closest relatives 'Sphaerisporangium dianthi' NEAU-CY18T (99.2 % 16S rRNA gene sequence similarity) and Sphaerisporangium cinnabarinum JCM 3291T (98.8 %).
30010529	5	56	theme	16S	657:659	arg1	NEAU-CY18T					638:647	Sphaerisporangium dianthi' NEAU-CY18T	611:647	Sphaerisporangium dianthi' NEAU-CY18T (99.2 % 16S rRNA gene sequence similarity)	611:690	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Sphaerisporangium and that it forms a monophyletic clade with its closest relatives 'Sphaerisporangium dianthi' NEAU-CY18T (99.2 % 16S rRNA gene sequence similarity) and Sphaerisporangium cinnabarinum JCM 3291T (98.8 %).
30010529	4	57	theme	Heilongjiang	317:328	arg1	Province					330:337	Heilongjiang Province	317:337	Heilongjiang Province	317:337	Arg) collected from Xianglu Mountain in Heilongjiang Province, north-east China, and characterized by using a polyphasic approach.
30010529	4	57	theme	Heilongjiang	317:328	arg1	China					351:355	north-east China	340:355	north-east China	340:355	Arg) collected from Xianglu Mountain in Heilongjiang Province, north-east China, and characterized by using a polyphasic approach.
30010529	13	58	theme	novel	1723:1727	arg1	species					1729:1735	a novel species	1721:1735	a novel species	1721:1735	Therefore, it is concluded that strain NEAU-mq3T represents a novel species of the genus Sphaerisporangium, for which the name Sphaerisporangium rhizosphaerae sp.
30010529	11	59	dep	identified	1336:1345	arg1	 0					1359:1360	 0	1359:1360	 0	1359:1360	The major fatty acids were identified as iso-C16 : 0, 10-methyl C17 : 0, C16 : 1ω7c and C17 : 1ω7c.
30010529	11	59	dep	identified	1336:1345	arg1	C17 					1373:1376	10-methyl C17 	1363:1376	10-methyl C17 	1363:1376	The major fatty acids were identified as iso-C16 : 0, 10-methyl C17 : 0, C16 : 1ω7c and C17 : 1ω7c.
30010529	3	60	theme	Hevea	251:255	arg1	brasiliensis					257:268	Hevea brasiliensis	251:268	Hevea brasiliensis Muell	251:274	A novel actinomycete, designated strain NEAU-mq3T, was isolated from the rhizosphere soil of a rubber tree (Hevea brasiliensis Muell.
30010529	6	61	theme	NEAU-mq3T	801:809	arg1	properties					780:789	Morphological and chemotaxonomic properties	747:789	Morphological and chemotaxonomic properties of strain NEAU-mq3T	747:809	Morphological and chemotaxonomic properties of strain NEAU-mq3T were also consistent with the description of the genus Sphaerisporangium.
30010529	5	62	theme	genus	520:524	arg1	Sphaerisporangium					526:542	the genus Sphaerisporangium	516:542	the genus Sphaerisporangium	516:542	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Sphaerisporangium and that it forms a monophyletic clade with its closest relatives 'Sphaerisporangium dianthi' NEAU-CY18T (99.2 % 16S rRNA gene sequence similarity) and Sphaerisporangium cinnabarinum JCM 3291T (98.8 %).
30010529	1	63	theme	Hevea	111:115	arg1	brasiliensis					117:128	a rubber tree (Hevea brasiliensis Muell	96:134	a rubber tree (Hevea brasiliensis Muell	96:134	nov., an actinomycete isolated from the rhizosphere soil of a rubber tree (Hevea brasiliensis Muell.
30010529	5	64	theme	Phylogenetic	408:419	arg1	analysis					421:428	Phylogenetic analysis	408:428	Phylogenetic analysis based on the 16S rRNA gene sequence	408:464	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Sphaerisporangium and that it forms a monophyletic clade with its closest relatives 'Sphaerisporangium dianthi' NEAU-CY18T (99.2 % 16S rRNA gene sequence similarity) and Sphaerisporangium cinnabarinum JCM 3291T (98.8 %).
30010529	12	65	theme	strain	1489:1494	arg1	NEAU-mq3T					1496:1504	strain NEAU-mq3T	1489:1504	strain NEAU-mq3T	1489:1504	DNA-DNA hybridization experiments and phenotypic tests were carried out between strain NEAU-mq3T and its most closely related strains, which further clarified their relatedness and demonstrated that NEAU-mq3T could be distinguished from these strains.
30010529	5	66	theme	%	655:655	arg1	similarity					680:689	99.2 % 16S rRNA gene sequence similarity	650:689	99.2 % 16S rRNA gene sequence similarity	650:689	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Sphaerisporangium and that it forms a monophyletic clade with its closest relatives 'Sphaerisporangium dianthi' NEAU-CY18T (99.2 % 16S rRNA gene sequence similarity) and Sphaerisporangium cinnabarinum JCM 3291T (98.8 %).
30010529	5	66	theme	%	655:655	arg1	NEAU-CY18T					638:647	Sphaerisporangium dianthi' NEAU-CY18T	611:647	Sphaerisporangium dianthi' NEAU-CY18T (99.2 % 16S rRNA gene sequence similarity)	611:690	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Sphaerisporangium and that it forms a monophyletic clade with its closest relatives 'Sphaerisporangium dianthi' NEAU-CY18T (99.2 % 16S rRNA gene sequence similarity) and Sphaerisporangium cinnabarinum JCM 3291T (98.8 %).
30010529	6	67	theme	strain	794:799	arg1	NEAU-mq3T					801:809	strain NEAU-mq3T	794:809	strain NEAU-mq3T	794:809	Morphological and chemotaxonomic properties of strain NEAU-mq3T were also consistent with the description of the genus Sphaerisporangium.
30010529	13	68	theme	rhizosphaerae	1806:1818	arg1	sp					1820:1821	the name Sphaerisporangium rhizosphaerae sp	1779:1821	the name Sphaerisporangium rhizosphaerae sp	1779:1821	Therefore, it is concluded that strain NEAU-mq3T represents a novel species of the genus Sphaerisporangium, for which the name Sphaerisporangium rhizosphaerae sp.
30010529	1	69	theme	brasiliensis	117:128	arg1	soil					88:91	the rhizosphere soil	72:91	the rhizosphere soil of a rubber tree (Hevea brasiliensis Muell	72:134	nov., an actinomycete isolated from the rhizosphere soil of a rubber tree (Hevea brasiliensis Muell.
30010529	13	70	theme	genus	1744:1748	arg1	Sphaerisporangium					1750:1766	the genus Sphaerisporangium	1740:1766	the genus Sphaerisporangium	1740:1766	Therefore, it is concluded that strain NEAU-mq3T represents a novel species of the genus Sphaerisporangium, for which the name Sphaerisporangium rhizosphaerae sp.
30010529	4	71	theme	polyphasic	387:396	arg1	approach					398:405	a polyphasic approach	385:405	a polyphasic approach	385:405	Arg) collected from Xianglu Mountain in Heilongjiang Province, north-east China, and characterized by using a polyphasic approach.
30010529	7	72	theme	whole-cell	889:898	arg1	madurose					912:919	madurose	912:919	madurose	912:919	The whole-cell sugars were madurose, mannose, ribose and glucose.
30010529	7	72	theme	whole-cell	889:898	arg1	sugars					900:905	The whole-cell sugars	885:905	The whole-cell sugars	885:905	The whole-cell sugars were madurose, mannose, ribose and glucose.
30010529	5	73	theme	JCM	727:729	arg1	relatives					600:608	its closest relatives	588:608	its closest relatives 'Sphaerisporangium dianthi' NEAU-CY18T (99.2 % 16S rRNA gene sequence similarity) and Sphaerisporangium cinnabarinum JCM 3291T (98.8 %)	588:744	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Sphaerisporangium and that it forms a monophyletic clade with its closest relatives 'Sphaerisporangium dianthi' NEAU-CY18T (99.2 % 16S rRNA gene sequence similarity) and Sphaerisporangium cinnabarinum JCM 3291T (98.8 %).
30010529	5	73	theme	JCM	727:729	arg1	3291T					731:735	Sphaerisporangium cinnabarinum JCM 3291T	696:735	Sphaerisporangium cinnabarinum JCM 3291T (98.8 %)	696:744	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Sphaerisporangium and that it forms a monophyletic clade with its closest relatives 'Sphaerisporangium dianthi' NEAU-CY18T (99.2 % 16S rRNA gene sequence similarity) and Sphaerisporangium cinnabarinum JCM 3291T (98.8 %).
30010529	5	73	theme	JCM	727:729	arg1	%					743:743	98.8 %	738:743	98.8 %	738:743	Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Sphaerisporangium and that it forms a monophyletic clade with its closest relatives 'Sphaerisporangium dianthi' NEAU-CY18T (99.2 % 16S rRNA gene sequence similarity) and Sphaerisporangium cinnabarinum JCM 3291T (98.8 %).
30010529	13	74	theme	strain	1693:1698	arg1	NEAU-mq3T					1700:1708	strain NEAU-mq3T	1693:1708	strain NEAU-mq3T	1693:1708	Therefore, it is concluded that strain NEAU-mq3T represents a novel species of the genus Sphaerisporangium, for which the name Sphaerisporangium rhizosphaerae sp.
30010529	10	75	theme	phosphatidylinositol	1215:1234	arg1	mannoside					1236:1244	phosphatidylinositol mannoside	1215:1244	phosphatidylinositol mannoside	1215:1244	The phospholipid profile consisted of diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol mannoside, an unidentified polar lipid and an unidentified phospholipid.
30098441	4	0	theme	activating	875:884	arg1	dectin-1					886:893	targeting and activating dectin-1	861:893	dectin-1	886:893	The aminated β-glucan plays dual roles as antigen presenting cells (APCs) targeted carrier and immunopotentiator (targeting and activating dectin-1 on APCs).
30098441	17	1	theme	adjuvant	2716:2723	arg1	vaccine					2708:2714	the dual targeting vaccine	2689:2714	the dual targeting vaccine adjuvant with aminated β-glucan and CpG-OND	2689:2758	By making use of synergistic effect of the dual targeting vaccine adjuvant with aminated β-glucan and CpG-OND, the nanoparticles induce robust antigen specific immune responses comparable to Freund's adjuvant without obvious toxicity.
30098441	17	2	theme	aminated	2730:2737	arg1	β-glucan					2739:2746	aminated β-glucan	2730:2746	aminated β-glucan	2730:2746	By making use of synergistic effect of the dual targeting vaccine adjuvant with aminated β-glucan and CpG-OND, the nanoparticles induce robust antigen specific immune responses comparable to Freund's adjuvant without obvious toxicity.
30098441	14	3	theme	TLR9	2410:2413	arg1	PAMP					2424:2427	a TLR9 targeted PAMP	2408:2427	a TLR9 targeted PAMP	2408:2427	β-Glucan (a dectin-1 and TLR2 targeted PAMP) and CpG-OND (a TLR9 targeted PAMP) are readily accessible.
30098441	14	3	theme	TLR9	2410:2413	arg1	CpG-OND					2399:2405	CpG-OND	2399:2405	CpG-OND (a TLR9 targeted PAMP)	2399:2428	β-Glucan (a dectin-1 and TLR2 targeted PAMP) and CpG-OND (a TLR9 targeted PAMP) are readily accessible.
30098441	3	4	theme	complexation	726:737	arg1	method					739:744	a simple and mild ionic complexation method	702:744	a simple and mild ionic complexation method	702:744	Here in this work, a CpG-oligodeoxynucleotides (CpG-OND) crosslinked aminated β-glucan-Ovalbumin dual targeting nanoparticle (CpG-OND-AG-OVA) is prepared through a simple and mild ionic complexation method.
30098441	11	5	theme	negligible	2028:2037	arg1	toxicity					2039:2046	negligible toxicity	2028:2046	negligible toxicity	2028:2046	Additionally, it should be easy to obtain and safe with negligible toxicity.
30098441	3	6	theme	mild	715:718	arg1	method					739:744	a simple and mild ionic complexation method	702:744	a simple and mild ionic complexation method	702:744	Here in this work, a CpG-oligodeoxynucleotides (CpG-OND) crosslinked aminated β-glucan-Ovalbumin dual targeting nanoparticle (CpG-OND-AG-OVA) is prepared through a simple and mild ionic complexation method.
30098441	5	7	from	targeting	990:998	arg1	APCs					1039:1042	APCs	1039:1042	APCs	1039:1042	Meanwhile, CpG-OND also plays dual roles as ionic crosslinker and immunopotentiator (targeting and activating Toll-like receptor 9 in APCs).
30098441	4	8	theme	targeting	861:869	arg1	dectin-1					886:893	targeting and activating dectin-1	861:893	dectin-1	886:893	The aminated β-glucan plays dual roles as antigen presenting cells (APCs) targeted carrier and immunopotentiator (targeting and activating dectin-1 on APCs).
30098441	14	9	theme	targeted	2380:2387	arg1	PAMP					2389:2392	a dectin-1 and TLR2 targeted PAMP	2360:2392	PAMP	2389:2392	β-Glucan (a dectin-1 and TLR2 targeted PAMP) and CpG-OND (a TLR9 targeted PAMP) are readily accessible.
30098441	10	10	theme	sustained	1812:1820	arg1	processing					1822:1831	sustained processing	1812:1831	sustained processing	1812:1831	STATEMENT OF SIGNIFICANCE An ideal adjuvant for subunit vaccine should act as both a carrier to enhance the uptake, sustained processing and cytosolic delivery of antigens, and an immunopotentiator to stimulate antigen presenting cells (APCs) for activation of naive T cells.
30098441	0	11	theme	cellular	128:135	arg1	responses					144:152	both humoral and cellular immune responses	111:152	both humoral and cellular immune responses	111:152	Synergistic effect of dual targeting vaccine adjuvant with aminated β-glucan and CpG-oligodeoxynucleotides for both humoral and cellular immune responses.
30098441	5	12	theme	ionic	949:953	arg1	crosslinker					955:965	ionic crosslinker	949:965	ionic crosslinker	949:965	Meanwhile, CpG-OND also plays dual roles as ionic crosslinker and immunopotentiator (targeting and activating Toll-like receptor 9 in APCs).
30098441	5	13	from	receptor	1025:1032	arg1	APCs					1039:1042	APCs	1039:1042	APCs	1039:1042	Meanwhile, CpG-OND also plays dual roles as ionic crosslinker and immunopotentiator (targeting and activating Toll-like receptor 9 in APCs).
30098441	16	14	theme	targeted	2622:2629	arg1	immunopotentiator					2631:2647	targeted immunopotentiator	2622:2647	targeted immunopotentiator	2622:2647	Meanwhile, CpG-OND also plays dual roles as crosslinker and APCs targeted immunopotentiator.
30098441	17	15	theme	effect	2679:2684	arg1	use					2660:2662	use	2660:2662	use of synergistic effect of the dual targeting vaccine adjuvant with aminated β-glucan and CpG-OND	2660:2758	By making use of synergistic effect of the dual targeting vaccine adjuvant with aminated β-glucan and CpG-OND, the nanoparticles induce robust antigen specific immune responses comparable to Freund's adjuvant without obvious toxicity.
30098441	8	16	theme	synergistic	1456:1466	arg1	effects					1468:1474	dual targeting synergistic effects	1441:1474	dual targeting synergistic effects between aminated β-glucan and CpG-OND	1441:1512	The potent adjuvant activity of the nanoparticles may originate from dual targeting synergistic effects between aminated β-glucan and CpG-OND.
30098441	12	17	theme	delivery	2138:2145	arg1	system					2147:2152	antigen delivery system	2130:2152	antigen delivery system	2130:2152	Unfortunately, both synthetic and natural polymers that have been developed into antigen delivery system cannot completely fulfill the requirements.
30098441	0	18	theme	dual	22:25	arg1	vaccine					37:43	dual targeting vaccine adjuvant	22:52	dual targeting vaccine adjuvant	22:52	Synergistic effect of dual targeting vaccine adjuvant with aminated β-glucan and CpG-oligodeoxynucleotides for both humoral and cellular immune responses.
30098441	5	19	dep	immunopotentiator	971:987	arg1	targeting					990:998	targeting	990:998	targeting	990:998	Meanwhile, CpG-OND also plays dual roles as ionic crosslinker and immunopotentiator (targeting and activating Toll-like receptor 9 in APCs).
30098441	5	19	dep	immunopotentiator	971:987	arg1	receptor					1025:1032	activating Toll-like receptor 9	1004:1034	activating Toll-like receptor 9 in APCs	1004:1042	Meanwhile, CpG-OND also plays dual roles as ionic crosslinker and immunopotentiator (targeting and activating Toll-like receptor 9 in APCs).
30098441	3	20	theme	CpG-oligodeoxynucleotides	561:585	arg1	nanoparticle					652:663	a CpG-oligodeoxynucleotides (CpG-OND) crosslinked aminated β-glucan-Ovalbumin dual targeting nanoparticle	559:663	a CpG-oligodeoxynucleotides (CpG-OND) crosslinked aminated β-glucan-Ovalbumin dual targeting nanoparticle (CpG-OND-AG-OVA)	559:680	Here in this work, a CpG-oligodeoxynucleotides (CpG-OND) crosslinked aminated β-glucan-Ovalbumin dual targeting nanoparticle (CpG-OND-AG-OVA) is prepared through a simple and mild ionic complexation method.
30098441	3	20	theme	CpG-oligodeoxynucleotides	561:585	arg1	CpG-OND-AG-OVA					666:679	CpG-OND-AG-OVA	666:679	CpG-OND-AG-OVA	666:679	Here in this work, a CpG-oligodeoxynucleotides (CpG-OND) crosslinked aminated β-glucan-Ovalbumin dual targeting nanoparticle (CpG-OND-AG-OVA) is prepared through a simple and mild ionic complexation method.
30098441	17	21	theme	targeting	2698:2706	arg1	vaccine					2708:2714	the dual targeting vaccine	2689:2714	the dual targeting vaccine adjuvant with aminated β-glucan and CpG-OND	2689:2758	By making use of synergistic effect of the dual targeting vaccine adjuvant with aminated β-glucan and CpG-OND, the nanoparticles induce robust antigen specific immune responses comparable to Freund's adjuvant without obvious toxicity.
30098441	3	22	theme	β-glucan-Ovalbumin	618:635	arg1	nanoparticle					652:663	a CpG-oligodeoxynucleotides (CpG-OND) crosslinked aminated β-glucan-Ovalbumin dual targeting nanoparticle	559:663	a CpG-oligodeoxynucleotides (CpG-OND) crosslinked aminated β-glucan-Ovalbumin dual targeting nanoparticle (CpG-OND-AG-OVA)	559:680	Here in this work, a CpG-oligodeoxynucleotides (CpG-OND) crosslinked aminated β-glucan-Ovalbumin dual targeting nanoparticle (CpG-OND-AG-OVA) is prepared through a simple and mild ionic complexation method.
30098441	3	22	theme	β-glucan-Ovalbumin	618:635	arg1	CpG-OND-AG-OVA					666:679	CpG-OND-AG-OVA	666:679	CpG-OND-AG-OVA	666:679	Here in this work, a CpG-oligodeoxynucleotides (CpG-OND) crosslinked aminated β-glucan-Ovalbumin dual targeting nanoparticle (CpG-OND-AG-OVA) is prepared through a simple and mild ionic complexation method.
30098441	9	23	theme	dual	1532:1535	arg1	vaccine					1580:1586	a promising vaccine adjuvant	1568:1595	a promising vaccine adjuvant for inducing robust humoral and cellular immune responses against infectious diseases and cancers	1568:1693	Accordingly, the dual targeting nanoparticles may be a promising vaccine adjuvant for inducing robust humoral and cellular immune responses against infectious diseases and cancers.
30098441	9	23	theme	dual	1532:1535	arg1	nanoparticles					1547:1559	the dual targeting nanoparticles	1528:1559	the dual targeting nanoparticles	1528:1559	Accordingly, the dual targeting nanoparticles may be a promising vaccine adjuvant for inducing robust humoral and cellular immune responses against infectious diseases and cancers.
30098441	17	24	theme	antigen	2793:2799	arg1	responses					2817:2825	robust antigen specific immune responses	2786:2825	robust antigen specific immune responses comparable to Freund's adjuvant without obvious toxicity	2786:2882	By making use of synergistic effect of the dual targeting vaccine adjuvant with aminated β-glucan and CpG-OND, the nanoparticles induce robust antigen specific immune responses comparable to Freund's adjuvant without obvious toxicity.
30098441	12	25	theme	synthetic	2069:2077	arg1	polymers					2091:2098	both synthetic and natural polymers	2064:2098	both synthetic and natural polymers that have been developed into antigen delivery system	2064:2152	Unfortunately, both synthetic and natural polymers that have been developed into antigen delivery system cannot completely fulfill the requirements.
30098441	0	26	theme	aminated	59:66	arg1	β-glucan					68:75	aminated β-glucan	59:75	aminated β-glucan	59:75	Synergistic effect of dual targeting vaccine adjuvant with aminated β-glucan and CpG-oligodeoxynucleotides for both humoral and cellular immune responses.
30098441	3	27	theme	crosslinked	597:607	arg1	nanoparticle					652:663	a CpG-oligodeoxynucleotides (CpG-OND) crosslinked aminated β-glucan-Ovalbumin dual targeting nanoparticle	559:663	a CpG-oligodeoxynucleotides (CpG-OND) crosslinked aminated β-glucan-Ovalbumin dual targeting nanoparticle (CpG-OND-AG-OVA)	559:680	Here in this work, a CpG-oligodeoxynucleotides (CpG-OND) crosslinked aminated β-glucan-Ovalbumin dual targeting nanoparticle (CpG-OND-AG-OVA) is prepared through a simple and mild ionic complexation method.
30098441	3	27	theme	crosslinked	597:607	arg1	CpG-OND-AG-OVA					666:679	CpG-OND-AG-OVA	666:679	CpG-OND-AG-OVA	666:679	Here in this work, a CpG-oligodeoxynucleotides (CpG-OND) crosslinked aminated β-glucan-Ovalbumin dual targeting nanoparticle (CpG-OND-AG-OVA) is prepared through a simple and mild ionic complexation method.
30098441	15	28	theme	Aminated	2454:2461	arg1	β-glucan					2463:2470	Aminated β-glucan	2454:2470	Aminated β-glucan	2454:2470	Aminated β-glucan plays dual roles in the nanoparticle as APCs targeted carrier and immunopotentiator.
30098441	12	29	theme	natural	2083:2089	arg1	polymers					2091:2098	both synthetic and natural polymers	2064:2098	both synthetic and natural polymers that have been developed into antigen delivery system	2064:2152	Unfortunately, both synthetic and natural polymers that have been developed into antigen delivery system cannot completely fulfill the requirements.
30098441	1	30	theme	approved	177:184	arg1	adjuvants					186:194	clinically approved adjuvants	166:194	clinically approved adjuvants (such as aluminum salts)	166:219	Presently, clinically approved adjuvants (such as aluminum salts) fail to induce cellular immune responses, which is crucial to defend against intracellular pathogens (including HIV, malaria, tuberculosis and Ebola) and cancer.
30098441	16	31	theme	dual	2587:2590	arg1	roles					2592:2596	dual roles	2587:2596	dual roles	2587:2596	Meanwhile, CpG-OND also plays dual roles as crosslinker and APCs targeted immunopotentiator.
30098441	16	31	theme	dual	2587:2590	arg1	APCs					2617:2620	APCs	2617:2620	APCs	2617:2620	Meanwhile, CpG-OND also plays dual roles as crosslinker and APCs targeted immunopotentiator.
30098441	2	32	theme	severe	517:522	arg1	reactions					529:537	severe side reactions	517:537	severe side reactions	517:537	However, Freund's complete adjuvant potently stimulates both humoral and cellular immune responses, accompanying by high toxicity and severe side reactions.
30098441	4	33	theme	dual	775:778	arg1	roles					780:784	dual roles	775:784	dual roles	775:784	The aminated β-glucan plays dual roles as antigen presenting cells (APCs) targeted carrier and immunopotentiator (targeting and activating dectin-1 on APCs).
30098441	4	34	theme	aminated	751:758	arg1	β-glucan					760:767	The aminated β-glucan	747:767	The aminated β-glucan	747:767	The aminated β-glucan plays dual roles as antigen presenting cells (APCs) targeted carrier and immunopotentiator (targeting and activating dectin-1 on APCs).
30098441	9	35	theme	promising	1570:1578	arg1	vaccine					1580:1586	a promising vaccine adjuvant	1568:1595	a promising vaccine adjuvant for inducing robust humoral and cellular immune responses against infectious diseases and cancers	1568:1693	Accordingly, the dual targeting nanoparticles may be a promising vaccine adjuvant for inducing robust humoral and cellular immune responses against infectious diseases and cancers.
30098441	9	35	theme	promising	1570:1578	arg1	nanoparticles					1547:1559	the dual targeting nanoparticles	1528:1559	the dual targeting nanoparticles	1528:1559	Accordingly, the dual targeting nanoparticles may be a promising vaccine adjuvant for inducing robust humoral and cellular immune responses against infectious diseases and cancers.
30098441	7	36	theme	proteolytic	1197:1207	arg1	processing					1209:1218	sustained proteolytic processing	1187:1218	sustained proteolytic processing	1187:1218	The particles significantly enhance uptake and sustained proteolytic processing of antigens, and result in APCs maturation, inducing robust Th1 and Th2-type immune responses comparable to Freund's adjuvant without obvious toxicity.
30098441	17	37	theme	specific	2801:2808	arg1	responses					2817:2825	robust antigen specific immune responses	2786:2825	robust antigen specific immune responses comparable to Freund's adjuvant without obvious toxicity	2786:2882	By making use of synergistic effect of the dual targeting vaccine adjuvant with aminated β-glucan and CpG-OND, the nanoparticles induce robust antigen specific immune responses comparable to Freund's adjuvant without obvious toxicity.
30098441	9	38	theme	adjuvant	1588:1595	arg1	vaccine					1580:1586	a promising vaccine adjuvant	1568:1595	a promising vaccine adjuvant for inducing robust humoral and cellular immune responses against infectious diseases and cancers	1568:1693	Accordingly, the dual targeting nanoparticles may be a promising vaccine adjuvant for inducing robust humoral and cellular immune responses against infectious diseases and cancers.
30098441	9	38	theme	adjuvant	1588:1595	arg1	nanoparticles					1547:1559	the dual targeting nanoparticles	1528:1559	the dual targeting nanoparticles	1528:1559	Accordingly, the dual targeting nanoparticles may be a promising vaccine adjuvant for inducing robust humoral and cellular immune responses against infectious diseases and cancers.
30098441	7	39	theme	adjuvant	1337:1344	arg1	's					1334:1335	Freund's	1328:1335	Freund's adjuvant without obvious toxicity	1328:1369	The particles significantly enhance uptake and sustained proteolytic processing of antigens, and result in APCs maturation, inducing robust Th1 and Th2-type immune responses comparable to Freund's adjuvant without obvious toxicity.
30098441	8	40	theme	potent	1376:1381	arg1	activity					1392:1399	The potent adjuvant activity	1372:1399	The potent adjuvant activity of the nanoparticles	1372:1420	The potent adjuvant activity of the nanoparticles may originate from dual targeting synergistic effects between aminated β-glucan and CpG-OND.
30098441	0	41	theme	Synergistic	0:10	arg1	effect					12:17	Synergistic effect	0:17	Synergistic effect of dual targeting vaccine adjuvant with aminated β-glucan and CpG-oligodeoxynucleotides for both humoral and cellular immune responses.	0:153	Synergistic effect of dual targeting vaccine adjuvant with aminated β-glucan and CpG-oligodeoxynucleotides for both humoral and cellular immune responses.
30098441	7	42	theme	Freund	1328:1333	arg1	's					1334:1335	Freund's	1328:1335	Freund's adjuvant without obvious toxicity	1328:1369	The particles significantly enhance uptake and sustained proteolytic processing of antigens, and result in APCs maturation, inducing robust Th1 and Th2-type immune responses comparable to Freund's adjuvant without obvious toxicity.
30098441	17	43	theme	robust	2786:2791	arg1	responses					2817:2825	robust antigen specific immune responses	2786:2825	robust antigen specific immune responses comparable to Freund's adjuvant without obvious toxicity	2786:2882	By making use of synergistic effect of the dual targeting vaccine adjuvant with aminated β-glucan and CpG-OND, the nanoparticles induce robust antigen specific immune responses comparable to Freund's adjuvant without obvious toxicity.
30098441	2	44	theme	complete	401:408	arg1	Freund					392:397	Freund's complete adjuvant	392:417	Freund's complete adjuvant	392:417	However, Freund's complete adjuvant potently stimulates both humoral and cellular immune responses, accompanying by high toxicity and severe side reactions.
30098441	15	45	theme	dual	2478:2481	arg1	roles					2483:2487	dual roles	2478:2487	dual roles	2478:2487	Aminated β-glucan plays dual roles in the nanoparticle as APCs targeted carrier and immunopotentiator.
30098441	9	46	theme	humoral	1617:1623	arg1	responses					1645:1653	robust humoral and cellular immune responses	1610:1653	robust humoral and cellular immune responses	1610:1653	Accordingly, the dual targeting nanoparticles may be a promising vaccine adjuvant for inducing robust humoral and cellular immune responses against infectious diseases and cancers.
30098441	7	47	theme	comparable	1314:1323	arg1	Th1					1280:1282	robust Th1 and Th2-type immune responses	1273:1312	Th1	1280:1282	The particles significantly enhance uptake and sustained proteolytic processing of antigens, and result in APCs maturation, inducing robust Th1 and Th2-type immune responses comparable to Freund's adjuvant without obvious toxicity.
30098441	2	48	theme	immune	465:470	arg1	responses					472:480	both humoral and cellular immune responses	439:480	both humoral and cellular immune responses	439:480	However, Freund's complete adjuvant potently stimulates both humoral and cellular immune responses, accompanying by high toxicity and severe side reactions.
30098441	0	49	theme	targeting	27:35	arg1	vaccine					37:43	dual targeting vaccine adjuvant	22:52	dual targeting vaccine adjuvant	22:52	Synergistic effect of dual targeting vaccine adjuvant with aminated β-glucan and CpG-oligodeoxynucleotides for both humoral and cellular immune responses.
30098441	1	50	theme	intracellular	298:310	arg1	malaria					338:344	malaria	338:344	malaria	338:344	Presently, clinically approved adjuvants (such as aluminum salts) fail to induce cellular immune responses, which is crucial to defend against intracellular pathogens (including HIV, malaria, tuberculosis and Ebola) and cancer.
30098441	1	50	theme	intracellular	298:310	arg1	pathogens					312:320	intracellular pathogens	298:320	intracellular pathogens (including HIV, malaria, tuberculosis and Ebola)	298:369	Presently, clinically approved adjuvants (such as aluminum salts) fail to induce cellular immune responses, which is crucial to defend against intracellular pathogens (including HIV, malaria, tuberculosis and Ebola) and cancer.
30098441	1	50	theme	intracellular	298:310	arg1	HIV					333:335	HIV	333:335	HIV	333:335	Presently, clinically approved adjuvants (such as aluminum salts) fail to induce cellular immune responses, which is crucial to defend against intracellular pathogens (including HIV, malaria, tuberculosis and Ebola) and cancer.
30098441	1	50	theme	intracellular	298:310	arg1	Ebola					364:368	Ebola	364:368	Ebola	364:368	Presently, clinically approved adjuvants (such as aluminum salts) fail to induce cellular immune responses, which is crucial to defend against intracellular pathogens (including HIV, malaria, tuberculosis and Ebola) and cancer.
30098441	1	50	theme	intracellular	298:310	arg1	tuberculosis					347:358	tuberculosis	347:358	tuberculosis	347:358	Presently, clinically approved adjuvants (such as aluminum salts) fail to induce cellular immune responses, which is crucial to defend against intracellular pathogens (including HIV, malaria, tuberculosis and Ebola) and cancer.
30098441	11	51	with	safe	2018:2021	arg1	toxicity					2039:2046	negligible toxicity	2028:2046	negligible toxicity	2028:2046	Additionally, it should be easy to obtain and safe with negligible toxicity.
30098441	9	52	theme	cellular	1629:1636	arg1	responses					1645:1653	robust humoral and cellular immune responses	1610:1653	robust humoral and cellular immune responses	1610:1653	Accordingly, the dual targeting nanoparticles may be a promising vaccine adjuvant for inducing robust humoral and cellular immune responses against infectious diseases and cancers.
30098441	7	53	theme	immune	1297:1302	arg1	responses					1304:1312	robust Th1 and Th2-type immune responses	1273:1312	responses	1304:1312	The particles significantly enhance uptake and sustained proteolytic processing of antigens, and result in APCs maturation, inducing robust Th1 and Th2-type immune responses comparable to Freund's adjuvant without obvious toxicity.
30098441	3	54	theme	targeting	642:650	arg1	nanoparticle					652:663	a CpG-oligodeoxynucleotides (CpG-OND) crosslinked aminated β-glucan-Ovalbumin dual targeting nanoparticle	559:663	a CpG-oligodeoxynucleotides (CpG-OND) crosslinked aminated β-glucan-Ovalbumin dual targeting nanoparticle (CpG-OND-AG-OVA)	559:680	Here in this work, a CpG-oligodeoxynucleotides (CpG-OND) crosslinked aminated β-glucan-Ovalbumin dual targeting nanoparticle (CpG-OND-AG-OVA) is prepared through a simple and mild ionic complexation method.
30098441	3	54	theme	targeting	642:650	arg1	CpG-OND-AG-OVA					666:679	CpG-OND-AG-OVA	666:679	CpG-OND-AG-OVA	666:679	Here in this work, a CpG-oligodeoxynucleotides (CpG-OND) crosslinked aminated β-glucan-Ovalbumin dual targeting nanoparticle (CpG-OND-AG-OVA) is prepared through a simple and mild ionic complexation method.
30098441	6	55	dep	in	1106:1107	arg1	vitro					1109:1113	vitro	1109:1113	vitro	1109:1113	The adjuvant activity of the particles is evaluated through in vitro and in vivo experiments.
30098441	10	56	theme	T	1963:1963	arg1	cells					1965:1969	naive T cells	1957:1969	naive T cells	1957:1969	STATEMENT OF SIGNIFICANCE An ideal adjuvant for subunit vaccine should act as both a carrier to enhance the uptake, sustained processing and cytosolic delivery of antigens, and an immunopotentiator to stimulate antigen presenting cells (APCs) for activation of naive T cells.
30098441	0	57	theme	adjuvant	45:52	arg1	vaccine					37:43	dual targeting vaccine adjuvant	22:52	dual targeting vaccine adjuvant	22:52	Synergistic effect of dual targeting vaccine adjuvant with aminated β-glucan and CpG-oligodeoxynucleotides for both humoral and cellular immune responses.
30098441	6	58	theme	in	1119:1120	arg1	experiments					1127:1137	in vitro and in vivo experiments	1106:1137	in vitro and in vivo experiments	1106:1137	The adjuvant activity of the particles is evaluated through in vitro and in vivo experiments.
30098441	10	59	theme	antigens	1859:1866	arg1	delivery					1847:1854	cytosolic delivery	1837:1854	cytosolic delivery	1837:1854	STATEMENT OF SIGNIFICANCE An ideal adjuvant for subunit vaccine should act as both a carrier to enhance the uptake, sustained processing and cytosolic delivery of antigens, and an immunopotentiator to stimulate antigen presenting cells (APCs) for activation of naive T cells.
30098441	10	59	theme	antigens	1859:1866	arg1	uptake					1804:1809	the uptake	1800:1809	the uptake	1800:1809	STATEMENT OF SIGNIFICANCE An ideal adjuvant for subunit vaccine should act as both a carrier to enhance the uptake, sustained processing and cytosolic delivery of antigens, and an immunopotentiator to stimulate antigen presenting cells (APCs) for activation of naive T cells.
30098441	10	59	theme	antigens	1859:1866	arg1	processing					1822:1831	sustained processing	1812:1831	sustained processing	1812:1831	STATEMENT OF SIGNIFICANCE An ideal adjuvant for subunit vaccine should act as both a carrier to enhance the uptake, sustained processing and cytosolic delivery of antigens, and an immunopotentiator to stimulate antigen presenting cells (APCs) for activation of naive T cells.
30098441	4	60	theme	presenting	797:806	arg1	cells					808:812	antigen presenting cells	789:812	antigen presenting cells (APCs) targeted carrier	789:836	The aminated β-glucan plays dual roles as antigen presenting cells (APCs) targeted carrier and immunopotentiator (targeting and activating dectin-1 on APCs).
30098441	4	60	theme	presenting	797:806	arg1	APCs					815:818	APCs	815:818	APCs	815:818	The aminated β-glucan plays dual roles as antigen presenting cells (APCs) targeted carrier and immunopotentiator (targeting and activating dectin-1 on APCs).
30098441	10	61	theme	subunit	1744:1750	arg1	vaccine					1752:1758	subunit vaccine	1744:1758	subunit vaccine	1744:1758	STATEMENT OF SIGNIFICANCE An ideal adjuvant for subunit vaccine should act as both a carrier to enhance the uptake, sustained processing and cytosolic delivery of antigens, and an immunopotentiator to stimulate antigen presenting cells (APCs) for activation of naive T cells.
30098441	16	62	dep	crosslinker	2601:2611	arg1	immunopotentiator					2631:2647	targeted immunopotentiator	2622:2647	targeted immunopotentiator	2622:2647	Meanwhile, CpG-OND also plays dual roles as crosslinker and APCs targeted immunopotentiator.
30098441	9	63	theme	infectious	1663:1672	arg1	diseases					1674:1681	infectious diseases	1663:1681	infectious diseases	1663:1681	Accordingly, the dual targeting nanoparticles may be a promising vaccine adjuvant for inducing robust humoral and cellular immune responses against infectious diseases and cancers.
30098441	10	64	theme	SIGNIFICANCE	1709:1720	arg1	STATEMENT					1696:1704	STATEMENT	1696:1704	STATEMENT OF SIGNIFICANCE An ideal adjuvant for subunit vaccine	1696:1758	STATEMENT OF SIGNIFICANCE An ideal adjuvant for subunit vaccine should act as both a carrier to enhance the uptake, sustained processing and cytosolic delivery of antigens, and an immunopotentiator to stimulate antigen presenting cells (APCs) for activation of naive T cells.
30098441	10	64	theme	SIGNIFICANCE	1709:1720	arg1	carrier					1781:1787	a carrier to enhance the uptake, sustained processing and cytosolic delivery of antigens	1779:1866	a carrier to enhance the uptake, sustained processing and cytosolic delivery of antigens	1779:1866	STATEMENT OF SIGNIFICANCE An ideal adjuvant for subunit vaccine should act as both a carrier to enhance the uptake, sustained processing and cytosolic delivery of antigens, and an immunopotentiator to stimulate antigen presenting cells (APCs) for activation of naive T cells.
30098441	10	64	theme	SIGNIFICANCE	1709:1720	arg1	immunopotentiator					1876:1892	an immunopotentiator to stimulate antigen presenting cells (APCs) for activation of naive T cells	1873:1969	an immunopotentiator to stimulate antigen presenting cells (APCs) for activation of naive T cells	1873:1969	STATEMENT OF SIGNIFICANCE An ideal adjuvant for subunit vaccine should act as both a carrier to enhance the uptake, sustained processing and cytosolic delivery of antigens, and an immunopotentiator to stimulate antigen presenting cells (APCs) for activation of naive T cells.
30098441	7	65	theme	robust	1273:1278	arg1	Th1					1280:1282	robust Th1 and Th2-type immune responses	1273:1312	Th1	1280:1282	The particles significantly enhance uptake and sustained proteolytic processing of antigens, and result in APCs maturation, inducing robust Th1 and Th2-type immune responses comparable to Freund's adjuvant without obvious toxicity.
30098441	14	66	dep	β-Glucan	2350:2357	arg1	PAMP					2389:2392	a dectin-1 and TLR2 targeted PAMP	2360:2392	PAMP	2389:2392	β-Glucan (a dectin-1 and TLR2 targeted PAMP) and CpG-OND (a TLR9 targeted PAMP) are readily accessible.
30098441	14	66	dep	β-Glucan	2350:2357	arg1	dectin-1					2362:2369	a dectin-1 and TLR2 targeted PAMP	2360:2392	dectin-1	2362:2369	β-Glucan (a dectin-1 and TLR2 targeted PAMP) and CpG-OND (a TLR9 targeted PAMP) are readily accessible.
30098441	10	67	theme	presenting	1915:1924	arg1	cells					1926:1930	antigen presenting cells	1907:1930	antigen presenting cells (APCs)	1907:1937	STATEMENT OF SIGNIFICANCE An ideal adjuvant for subunit vaccine should act as both a carrier to enhance the uptake, sustained processing and cytosolic delivery of antigens, and an immunopotentiator to stimulate antigen presenting cells (APCs) for activation of naive T cells.
30098441	10	67	theme	presenting	1915:1924	arg1	APCs					1933:1936	APCs	1933:1936	APCs	1933:1936	STATEMENT OF SIGNIFICANCE An ideal adjuvant for subunit vaccine should act as both a carrier to enhance the uptake, sustained processing and cytosolic delivery of antigens, and an immunopotentiator to stimulate antigen presenting cells (APCs) for activation of naive T cells.
30098441	8	68	theme	targeting	1446:1454	arg1	effects					1468:1474	dual targeting synergistic effects	1441:1474	dual targeting synergistic effects between aminated β-glucan and CpG-OND	1441:1512	The potent adjuvant activity of the nanoparticles may originate from dual targeting synergistic effects between aminated β-glucan and CpG-OND.
30098441	1	69	theme	cellular	236:243	arg1	responses					252:260	cellular immune responses	236:260	cellular immune responses	236:260	Presently, clinically approved adjuvants (such as aluminum salts) fail to induce cellular immune responses, which is crucial to defend against intracellular pathogens (including HIV, malaria, tuberculosis and Ebola) and cancer.
30098441	5	70	theme	Toll-like	1015:1023	arg1	receptor					1025:1032	activating Toll-like receptor 9	1004:1034	activating Toll-like receptor 9 in APCs	1004:1042	Meanwhile, CpG-OND also plays dual roles as ionic crosslinker and immunopotentiator (targeting and activating Toll-like receptor 9 in APCs).
30098441	10	71	theme	naive	1957:1961	arg1	cells					1965:1969	naive T cells	1957:1969	naive T cells	1957:1969	STATEMENT OF SIGNIFICANCE An ideal adjuvant for subunit vaccine should act as both a carrier to enhance the uptake, sustained processing and cytosolic delivery of antigens, and an immunopotentiator to stimulate antigen presenting cells (APCs) for activation of naive T cells.
30098441	17	72	with	adjuvant	2716:2723	arg1	CpG-OND					2752:2758	CpG-OND	2752:2758	CpG-OND	2752:2758	By making use of synergistic effect of the dual targeting vaccine adjuvant with aminated β-glucan and CpG-OND, the nanoparticles induce robust antigen specific immune responses comparable to Freund's adjuvant without obvious toxicity.
30098441	17	72	with	adjuvant	2716:2723	arg1	β-glucan					2739:2746	aminated β-glucan	2730:2746	aminated β-glucan	2730:2746	By making use of synergistic effect of the dual targeting vaccine adjuvant with aminated β-glucan and CpG-OND, the nanoparticles induce robust antigen specific immune responses comparable to Freund's adjuvant without obvious toxicity.
30098441	7	73	theme	APCs	1247:1250	arg1	maturation					1252:1261	APCs maturation	1247:1261	APCs maturation	1247:1261	The particles significantly enhance uptake and sustained proteolytic processing of antigens, and result in APCs maturation, inducing robust Th1 and Th2-type immune responses comparable to Freund's adjuvant without obvious toxicity.
30098441	1	74	dep	adjuvants	186:194	arg1	such					197:200	such	197:200	such	197:200	Presently, clinically approved adjuvants (such as aluminum salts) fail to induce cellular immune responses, which is crucial to defend against intracellular pathogens (including HIV, malaria, tuberculosis and Ebola) and cancer.
30098441	10	75	theme	cells	1965:1969	arg1	activation					1943:1952	activation	1943:1952	activation of naive T cells	1943:1969	STATEMENT OF SIGNIFICANCE An ideal adjuvant for subunit vaccine should act as both a carrier to enhance the uptake, sustained processing and cytosolic delivery of antigens, and an immunopotentiator to stimulate antigen presenting cells (APCs) for activation of naive T cells.
30098441	17	76	theme	comparable	2827:2836	arg1	responses					2817:2825	robust antigen specific immune responses	2786:2825	robust antigen specific immune responses comparable to Freund's adjuvant without obvious toxicity	2786:2882	By making use of synergistic effect of the dual targeting vaccine adjuvant with aminated β-glucan and CpG-OND, the nanoparticles induce robust antigen specific immune responses comparable to Freund's adjuvant without obvious toxicity.
30098441	3	77	theme	ionic	720:724	arg1	method					739:744	a simple and mild ionic complexation method	702:744	a simple and mild ionic complexation method	702:744	Here in this work, a CpG-oligodeoxynucleotides (CpG-OND) crosslinked aminated β-glucan-Ovalbumin dual targeting nanoparticle (CpG-OND-AG-OVA) is prepared through a simple and mild ionic complexation method.
30098441	0	78	theme	immune	137:142	arg1	responses					144:152	both humoral and cellular immune responses	111:152	both humoral and cellular immune responses	111:152	Synergistic effect of dual targeting vaccine adjuvant with aminated β-glucan and CpG-oligodeoxynucleotides for both humoral and cellular immune responses.
30098441	4	79	theme	targeted	821:828	arg1	carrier					830:836	antigen presenting cells (APCs) targeted carrier	789:836	antigen presenting cells (APCs) targeted carrier	789:836	The aminated β-glucan plays dual roles as antigen presenting cells (APCs) targeted carrier and immunopotentiator (targeting and activating dectin-1 on APCs).
30098441	1	80	theme	aluminum	205:212	arg1	salts					214:218	aluminum salts	205:218	aluminum salts	205:218	Presently, clinically approved adjuvants (such as aluminum salts) fail to induce cellular immune responses, which is crucial to defend against intracellular pathogens (including HIV, malaria, tuberculosis and Ebola) and cancer.
30098441	4	81	theme	cells	808:812	arg1	carrier					830:836	antigen presenting cells (APCs) targeted carrier	789:836	antigen presenting cells (APCs) targeted carrier	789:836	The aminated β-glucan plays dual roles as antigen presenting cells (APCs) targeted carrier and immunopotentiator (targeting and activating dectin-1 on APCs).
30098441	17	82	theme	synergistic	2667:2677	arg1	effect					2679:2684	synergistic effect	2667:2684	synergistic effect of the dual targeting vaccine adjuvant with aminated β-glucan and CpG-OND	2667:2758	By making use of synergistic effect of the dual targeting vaccine adjuvant with aminated β-glucan and CpG-OND, the nanoparticles induce robust antigen specific immune responses comparable to Freund's adjuvant without obvious toxicity.
30098441	2	83	theme	side	524:527	arg1	reactions					529:537	severe side reactions	517:537	severe side reactions	517:537	However, Freund's complete adjuvant potently stimulates both humoral and cellular immune responses, accompanying by high toxicity and severe side reactions.
30098441	8	84	theme	aminated	1484:1491	arg1	β-glucan					1493:1500	aminated β-glucan	1484:1500	aminated β-glucan	1484:1500	The potent adjuvant activity of the nanoparticles may originate from dual targeting synergistic effects between aminated β-glucan and CpG-OND.
30098441	12	85	theme	antigen	2130:2136	arg1	system					2147:2152	antigen delivery system	2130:2152	antigen delivery system	2130:2152	Unfortunately, both synthetic and natural polymers that have been developed into antigen delivery system cannot completely fulfill the requirements.
30098441	10	86	theme	cytosolic	1837:1845	arg1	delivery					1847:1854	cytosolic delivery	1837:1854	cytosolic delivery	1837:1854	STATEMENT OF SIGNIFICANCE An ideal adjuvant for subunit vaccine should act as both a carrier to enhance the uptake, sustained processing and cytosolic delivery of antigens, and an immunopotentiator to stimulate antigen presenting cells (APCs) for activation of naive T cells.
30098441	17	87	theme	dual	2693:2696	arg1	vaccine					2708:2714	the dual targeting vaccine	2689:2714	the dual targeting vaccine adjuvant with aminated β-glucan and CpG-OND	2689:2758	By making use of synergistic effect of the dual targeting vaccine adjuvant with aminated β-glucan and CpG-OND, the nanoparticles induce robust antigen specific immune responses comparable to Freund's adjuvant without obvious toxicity.
30098441	14	88	theme	TLR2	2375:2378	arg1	PAMP					2389:2392	a dectin-1 and TLR2 targeted PAMP	2360:2392	PAMP	2389:2392	β-Glucan (a dectin-1 and TLR2 targeted PAMP) and CpG-OND (a TLR9 targeted PAMP) are readily accessible.
30098441	3	89	theme	aminated	609:616	arg1	nanoparticle					652:663	a CpG-oligodeoxynucleotides (CpG-OND) crosslinked aminated β-glucan-Ovalbumin dual targeting nanoparticle	559:663	a CpG-oligodeoxynucleotides (CpG-OND) crosslinked aminated β-glucan-Ovalbumin dual targeting nanoparticle (CpG-OND-AG-OVA)	559:680	Here in this work, a CpG-oligodeoxynucleotides (CpG-OND) crosslinked aminated β-glucan-Ovalbumin dual targeting nanoparticle (CpG-OND-AG-OVA) is prepared through a simple and mild ionic complexation method.
30098441	3	89	theme	aminated	609:616	arg1	CpG-OND-AG-OVA					666:679	CpG-OND-AG-OVA	666:679	CpG-OND-AG-OVA	666:679	Here in this work, a CpG-oligodeoxynucleotides (CpG-OND) crosslinked aminated β-glucan-Ovalbumin dual targeting nanoparticle (CpG-OND-AG-OVA) is prepared through a simple and mild ionic complexation method.
30098441	13	90	theme	simple	2326:2331	arg1	method					2342:2347	a simple and mild method	2324:2347	a simple and mild method	2324:2347	In the present study, the authors design nanoparticles with aminated β-glucan and CpG-oligodeoxynucleotides (CpG-OND) through a simple and mild method.
30098441	17	91	theme	vaccine	2708:2714	arg1	effect					2679:2684	synergistic effect	2667:2684	synergistic effect of the dual targeting vaccine adjuvant with aminated β-glucan and CpG-OND	2667:2758	By making use of synergistic effect of the dual targeting vaccine adjuvant with aminated β-glucan and CpG-OND, the nanoparticles induce robust antigen specific immune responses comparable to Freund's adjuvant without obvious toxicity.
30098441	13	92	theme	mild	2337:2340	arg1	method					2342:2347	a simple and mild method	2324:2347	a simple and mild method	2324:2347	In the present study, the authors design nanoparticles with aminated β-glucan and CpG-oligodeoxynucleotides (CpG-OND) through a simple and mild method.
30098441	9	93	theme	targeting	1537:1545	arg1	vaccine					1580:1586	a promising vaccine adjuvant	1568:1595	a promising vaccine adjuvant for inducing robust humoral and cellular immune responses against infectious diseases and cancers	1568:1693	Accordingly, the dual targeting nanoparticles may be a promising vaccine adjuvant for inducing robust humoral and cellular immune responses against infectious diseases and cancers.
30098441	9	93	theme	targeting	1537:1545	arg1	nanoparticles					1547:1559	the dual targeting nanoparticles	1528:1559	the dual targeting nanoparticles	1528:1559	Accordingly, the dual targeting nanoparticles may be a promising vaccine adjuvant for inducing robust humoral and cellular immune responses against infectious diseases and cancers.
30098441	17	94	theme	Freund	2841:2846	arg1	's					2847:2848	Freund's	2841:2848	Freund's adjuvant without obvious toxicity	2841:2882	By making use of synergistic effect of the dual targeting vaccine adjuvant with aminated β-glucan and CpG-OND, the nanoparticles induce robust antigen specific immune responses comparable to Freund's adjuvant without obvious toxicity.
30098441	7	95	theme	obvious	1354:1360	arg1	toxicity					1362:1369	obvious toxicity	1354:1369	obvious toxicity	1354:1369	The particles significantly enhance uptake and sustained proteolytic processing of antigens, and result in APCs maturation, inducing robust Th1 and Th2-type immune responses comparable to Freund's adjuvant without obvious toxicity.
30098441	7	96	theme	antigens	1223:1230	arg1	processing					1209:1218	sustained proteolytic processing	1187:1218	sustained proteolytic processing	1187:1218	The particles significantly enhance uptake and sustained proteolytic processing of antigens, and result in APCs maturation, inducing robust Th1 and Th2-type immune responses comparable to Freund's adjuvant without obvious toxicity.
30098441	7	96	theme	antigens	1223:1230	arg1	uptake					1176:1181	uptake	1176:1181	uptake	1176:1181	The particles significantly enhance uptake and sustained proteolytic processing of antigens, and result in APCs maturation, inducing robust Th1 and Th2-type immune responses comparable to Freund's adjuvant without obvious toxicity.
30098441	4	97	from	dectin-1	886:893	arg1	APCs					898:901	APCs	898:901	APCs	898:901	The aminated β-glucan plays dual roles as antigen presenting cells (APCs) targeted carrier and immunopotentiator (targeting and activating dectin-1 on APCs).
30098441	17	98	theme	adjuvant	2850:2857	arg1	's					2847:2848	Freund's	2841:2848	Freund's adjuvant without obvious toxicity	2841:2882	By making use of synergistic effect of the dual targeting vaccine adjuvant with aminated β-glucan and CpG-OND, the nanoparticles induce robust antigen specific immune responses comparable to Freund's adjuvant without obvious toxicity.
30098441	17	99	theme	obvious	2867:2873	arg1	toxicity					2875:2882	obvious toxicity	2867:2882	obvious toxicity	2867:2882	By making use of synergistic effect of the dual targeting vaccine adjuvant with aminated β-glucan and CpG-OND, the nanoparticles induce robust antigen specific immune responses comparable to Freund's adjuvant without obvious toxicity.
30098441	7	100	theme	sustained	1187:1195	arg1	processing					1209:1218	sustained proteolytic processing	1187:1218	sustained proteolytic processing	1187:1218	The particles significantly enhance uptake and sustained proteolytic processing of antigens, and result in APCs maturation, inducing robust Th1 and Th2-type immune responses comparable to Freund's adjuvant without obvious toxicity.
30098441	6	101	theme	particles	1075:1083	arg1	activity					1059:1066	The adjuvant activity	1046:1066	The adjuvant activity of the particles	1046:1083	The adjuvant activity of the particles is evaluated through in vitro and in vivo experiments.
30098441	2	102	theme	high	499:502	arg1	toxicity					504:511	high toxicity	499:511	high toxicity	499:511	However, Freund's complete adjuvant potently stimulates both humoral and cellular immune responses, accompanying by high toxicity and severe side reactions.
30098441	17	103	theme	immune	2810:2815	arg1	responses					2817:2825	robust antigen specific immune responses	2786:2825	robust antigen specific immune responses comparable to Freund's adjuvant without obvious toxicity	2786:2882	By making use of synergistic effect of the dual targeting vaccine adjuvant with aminated β-glucan and CpG-OND, the nanoparticles induce robust antigen specific immune responses comparable to Freund's adjuvant without obvious toxicity.
30098441	5	104	theme	dual	935:938	arg1	roles					940:944	dual roles	935:944	dual roles	935:944	Meanwhile, CpG-OND also plays dual roles as ionic crosslinker and immunopotentiator (targeting and activating Toll-like receptor 9 in APCs).
30098441	3	105	link	crosslinked	597:607	arg1	nanoparticle					652:663	a CpG-oligodeoxynucleotides (CpG-OND) crosslinked aminated β-glucan-Ovalbumin dual targeting nanoparticle	559:663	a CpG-oligodeoxynucleotides (CpG-OND) crosslinked aminated β-glucan-Ovalbumin dual targeting nanoparticle (CpG-OND-AG-OVA)	559:680	Here in this work, a CpG-oligodeoxynucleotides (CpG-OND) crosslinked aminated β-glucan-Ovalbumin dual targeting nanoparticle (CpG-OND-AG-OVA) is prepared through a simple and mild ionic complexation method.
30098441	3	105	link	crosslinked	597:607	arg1	CpG-OND-AG-OVA					666:679	CpG-OND-AG-OVA	666:679	CpG-OND-AG-OVA	666:679	Here in this work, a CpG-oligodeoxynucleotides (CpG-OND) crosslinked aminated β-glucan-Ovalbumin dual targeting nanoparticle (CpG-OND-AG-OVA) is prepared through a simple and mild ionic complexation method.
30098441	4	106	dep	immunopotentiator	842:858	arg1	dectin-1					886:893	targeting and activating dectin-1	861:893	dectin-1	886:893	The aminated β-glucan plays dual roles as antigen presenting cells (APCs) targeted carrier and immunopotentiator (targeting and activating dectin-1 on APCs).
30098441	13	107	theme	aminated	2258:2265	arg1	β-glucan					2267:2274	aminated β-glucan	2258:2274	aminated β-glucan	2258:2274	In the present study, the authors design nanoparticles with aminated β-glucan and CpG-oligodeoxynucleotides (CpG-OND) through a simple and mild method.
30098441	9	108	theme	robust	1610:1615	arg1	responses					1645:1653	robust humoral and cellular immune responses	1610:1653	robust humoral and cellular immune responses	1610:1653	Accordingly, the dual targeting nanoparticles may be a promising vaccine adjuvant for inducing robust humoral and cellular immune responses against infectious diseases and cancers.
30098441	6	109	theme	adjuvant	1050:1057	arg1	activity					1059:1066	The adjuvant activity	1046:1066	The adjuvant activity of the particles	1046:1083	The adjuvant activity of the particles is evaluated through in vitro and in vivo experiments.
30098441	2	110	theme	adjuvant	410:417	arg1	Freund					392:397	Freund's complete adjuvant	392:417	Freund's complete adjuvant	392:417	However, Freund's complete adjuvant potently stimulates both humoral and cellular immune responses, accompanying by high toxicity and severe side reactions.
30098441	10	111	theme	antigen	1907:1913	arg1	cells					1926:1930	antigen presenting cells	1907:1930	antigen presenting cells (APCs)	1907:1937	STATEMENT OF SIGNIFICANCE An ideal adjuvant for subunit vaccine should act as both a carrier to enhance the uptake, sustained processing and cytosolic delivery of antigens, and an immunopotentiator to stimulate antigen presenting cells (APCs) for activation of naive T cells.
30098441	10	111	theme	antigen	1907:1913	arg1	APCs					1933:1936	APCs	1933:1936	APCs	1933:1936	STATEMENT OF SIGNIFICANCE An ideal adjuvant for subunit vaccine should act as both a carrier to enhance the uptake, sustained processing and cytosolic delivery of antigens, and an immunopotentiator to stimulate antigen presenting cells (APCs) for activation of naive T cells.
30098441	8	112	theme	adjuvant	1383:1390	arg1	activity					1392:1399	The potent adjuvant activity	1372:1399	The potent adjuvant activity of the nanoparticles	1372:1420	The potent adjuvant activity of the nanoparticles may originate from dual targeting synergistic effects between aminated β-glucan and CpG-OND.
30098441	0	113	theme	vaccine	37:43	arg1	effect					12:17	Synergistic effect	0:17	Synergistic effect of dual targeting vaccine adjuvant with aminated β-glucan and CpG-oligodeoxynucleotides for both humoral and cellular immune responses.	0:153	Synergistic effect of dual targeting vaccine adjuvant with aminated β-glucan and CpG-oligodeoxynucleotides for both humoral and cellular immune responses.
30098441	7	114	theme	Th2-type	1288:1295	arg1	responses					1304:1312	robust Th1 and Th2-type immune responses	1273:1312	responses	1304:1312	The particles significantly enhance uptake and sustained proteolytic processing of antigens, and result in APCs maturation, inducing robust Th1 and Th2-type immune responses comparable to Freund's adjuvant without obvious toxicity.
30098441	2	115	theme	cellular	456:463	arg1	responses					472:480	both humoral and cellular immune responses	439:480	both humoral and cellular immune responses	439:480	However, Freund's complete adjuvant potently stimulates both humoral and cellular immune responses, accompanying by high toxicity and severe side reactions.
30098441	3	116	theme	dual	637:640	arg1	nanoparticle					652:663	a CpG-oligodeoxynucleotides (CpG-OND) crosslinked aminated β-glucan-Ovalbumin dual targeting nanoparticle	559:663	a CpG-oligodeoxynucleotides (CpG-OND) crosslinked aminated β-glucan-Ovalbumin dual targeting nanoparticle (CpG-OND-AG-OVA)	559:680	Here in this work, a CpG-oligodeoxynucleotides (CpG-OND) crosslinked aminated β-glucan-Ovalbumin dual targeting nanoparticle (CpG-OND-AG-OVA) is prepared through a simple and mild ionic complexation method.
30098441	3	116	theme	dual	637:640	arg1	CpG-OND-AG-OVA					666:679	CpG-OND-AG-OVA	666:679	CpG-OND-AG-OVA	666:679	Here in this work, a CpG-oligodeoxynucleotides (CpG-OND) crosslinked aminated β-glucan-Ovalbumin dual targeting nanoparticle (CpG-OND-AG-OVA) is prepared through a simple and mild ionic complexation method.
30098441	10	117	dep	SIGNIFICANCE	1709:1720	arg1	adjuvant					1731:1738	adjuvant	1731:1738	adjuvant	1731:1738	STATEMENT OF SIGNIFICANCE An ideal adjuvant for subunit vaccine should act as both a carrier to enhance the uptake, sustained processing and cytosolic delivery of antigens, and an immunopotentiator to stimulate antigen presenting cells (APCs) for activation of naive T cells.
30098441	0	118	theme	humoral	116:122	arg1	responses					144:152	both humoral and cellular immune responses	111:152	both humoral and cellular immune responses	111:152	Synergistic effect of dual targeting vaccine adjuvant with aminated β-glucan and CpG-oligodeoxynucleotides for both humoral and cellular immune responses.
30098441	13	119	theme	authors	2224:2230	arg1	design					2232:2237	the authors design	2220:2237	the authors design	2220:2237	In the present study, the authors design nanoparticles with aminated β-glucan and CpG-oligodeoxynucleotides (CpG-OND) through a simple and mild method.
30098441	9	120	theme	immune	1638:1643	arg1	responses					1645:1653	robust humoral and cellular immune responses	1610:1653	robust humoral and cellular immune responses	1610:1653	Accordingly, the dual targeting nanoparticles may be a promising vaccine adjuvant for inducing robust humoral and cellular immune responses against infectious diseases and cancers.
30098441	2	121	theme	humoral	444:450	arg1	responses					472:480	both humoral and cellular immune responses	439:480	both humoral and cellular immune responses	439:480	However, Freund's complete adjuvant potently stimulates both humoral and cellular immune responses, accompanying by high toxicity and severe side reactions.
30098441	8	122	theme	nanoparticles	1408:1420	arg1	activity					1392:1399	The potent adjuvant activity	1372:1399	The potent adjuvant activity of the nanoparticles	1372:1420	The potent adjuvant activity of the nanoparticles may originate from dual targeting synergistic effects between aminated β-glucan and CpG-OND.
30098441	0	123	with	effect	12:17	arg1	CpG-oligodeoxynucleotides					81:105	CpG-oligodeoxynucleotides	81:105	CpG-oligodeoxynucleotides	81:105	Synergistic effect of dual targeting vaccine adjuvant with aminated β-glucan and CpG-oligodeoxynucleotides for both humoral and cellular immune responses.
30098441	0	123	with	effect	12:17	arg1	β-glucan					68:75	aminated β-glucan	59:75	aminated β-glucan	59:75	Synergistic effect of dual targeting vaccine adjuvant with aminated β-glucan and CpG-oligodeoxynucleotides for both humoral and cellular immune responses.
30098441	6	124	dep	in	1119:1120	arg1	vivo					1122:1125	vivo	1122:1125	vivo	1122:1125	The adjuvant activity of the particles is evaluated through in vitro and in vivo experiments.
30098441	3	125	theme	CpG-OND	588:594	arg1	nanoparticle					652:663	a CpG-oligodeoxynucleotides (CpG-OND) crosslinked aminated β-glucan-Ovalbumin dual targeting nanoparticle	559:663	a CpG-oligodeoxynucleotides (CpG-OND) crosslinked aminated β-glucan-Ovalbumin dual targeting nanoparticle (CpG-OND-AG-OVA)	559:680	Here in this work, a CpG-oligodeoxynucleotides (CpG-OND) crosslinked aminated β-glucan-Ovalbumin dual targeting nanoparticle (CpG-OND-AG-OVA) is prepared through a simple and mild ionic complexation method.
30098441	3	125	theme	CpG-OND	588:594	arg1	CpG-OND-AG-OVA					666:679	CpG-OND-AG-OVA	666:679	CpG-OND-AG-OVA	666:679	Here in this work, a CpG-oligodeoxynucleotides (CpG-OND) crosslinked aminated β-glucan-Ovalbumin dual targeting nanoparticle (CpG-OND-AG-OVA) is prepared through a simple and mild ionic complexation method.
30098441	4	126	theme	antigen	789:795	arg1	cells					808:812	antigen presenting cells	789:812	antigen presenting cells (APCs) targeted carrier	789:836	The aminated β-glucan plays dual roles as antigen presenting cells (APCs) targeted carrier and immunopotentiator (targeting and activating dectin-1 on APCs).
30098441	4	126	theme	antigen	789:795	arg1	APCs					815:818	APCs	815:818	APCs	815:818	The aminated β-glucan plays dual roles as antigen presenting cells (APCs) targeted carrier and immunopotentiator (targeting and activating dectin-1 on APCs).
30098441	13	127	theme	present	2205:2211	arg1	study					2213:2217	the present study	2201:2217	the present study	2201:2217	In the present study, the authors design nanoparticles with aminated β-glucan and CpG-oligodeoxynucleotides (CpG-OND) through a simple and mild method.
30098441	5	128	theme	activating	1004:1013	arg1	receptor					1025:1032	activating Toll-like receptor 9	1004:1034	activating Toll-like receptor 9 in APCs	1004:1042	Meanwhile, CpG-OND also plays dual roles as ionic crosslinker and immunopotentiator (targeting and activating Toll-like receptor 9 in APCs).
30098441	6	129	theme	in	1106:1107	arg1	experiments					1127:1137	in vitro and in vivo experiments	1106:1137	in vitro and in vivo experiments	1106:1137	The adjuvant activity of the particles is evaluated through in vitro and in vivo experiments.
30098441	3	130	theme	simple	704:709	arg1	method					739:744	a simple and mild ionic complexation method	702:744	a simple and mild ionic complexation method	702:744	Here in this work, a CpG-oligodeoxynucleotides (CpG-OND) crosslinked aminated β-glucan-Ovalbumin dual targeting nanoparticle (CpG-OND-AG-OVA) is prepared through a simple and mild ionic complexation method.
30098441	8	131	theme	dual	1441:1444	arg1	effects					1468:1474	dual targeting synergistic effects	1441:1474	dual targeting synergistic effects between aminated β-glucan and CpG-OND	1441:1512	The potent adjuvant activity of the nanoparticles may originate from dual targeting synergistic effects between aminated β-glucan and CpG-OND.
30098441	14	132	theme	targeted	2415:2422	arg1	PAMP					2424:2427	a TLR9 targeted PAMP	2408:2427	a TLR9 targeted PAMP	2408:2427	β-Glucan (a dectin-1 and TLR2 targeted PAMP) and CpG-OND (a TLR9 targeted PAMP) are readily accessible.
30098441	14	132	theme	targeted	2415:2422	arg1	CpG-OND					2399:2405	CpG-OND	2399:2405	CpG-OND (a TLR9 targeted PAMP)	2399:2428	β-Glucan (a dectin-1 and TLR2 targeted PAMP) and CpG-OND (a TLR9 targeted PAMP) are readily accessible.
30098441	1	133	theme	immune	245:250	arg1	responses					252:260	cellular immune responses	236:260	cellular immune responses	236:260	Presently, clinically approved adjuvants (such as aluminum salts) fail to induce cellular immune responses, which is crucial to defend against intracellular pathogens (including HIV, malaria, tuberculosis and Ebola) and cancer.
30946611	9	0	theme	proteins	1549:1556	arg1	expression					1532:1541	expression	1532:1541	expression of TJ proteins (p < .05) by Caco-2 cells	1532:1582	Moreover, OSM downregulated expression of TJ proteins (p < .05) by Caco-2 cells in a concentration-dependent manner.
30946611	3	1	theme	3	437:437	arg1	%					438:438	%	438:438	%	438:438	Methods: In the murine model of 3% dextran sulfate sodium (DSS)-induced colitis, we tested disease activity index (DAI), colon length, histological score and expression of tight junction (TJ) proteins (ZO-1, occludin and claudin-1), OSM, TNF-α and TLR5.
30946611	1	2	theme	anti-TNF	232:239	arg1	agents					241:246	anti-TNF agents	232:246	anti-TNF agents used for IBD therapy	232:267	Objective: Levels of oncostatin M (OSM) and the composition of gut microbiota predict responses to anti-TNF agents used for IBD therapy.
30946611	3	3	theme	proteins	597:604	arg1	TLR5					653:656	TLR5	653:656	TLR5	653:656	Methods: In the murine model of 3% dextran sulfate sodium (DSS)-induced colitis, we tested disease activity index (DAI), colon length, histological score and expression of tight junction (TJ) proteins (ZO-1, occludin and claudin-1), OSM, TNF-α and TLR5.
30946611	3	3	theme	proteins	597:604	arg1	length					532:537	colon length	526:537	colon length	526:537	Methods: In the murine model of 3% dextran sulfate sodium (DSS)-induced colitis, we tested disease activity index (DAI), colon length, histological score and expression of tight junction (TJ) proteins (ZO-1, occludin and claudin-1), OSM, TNF-α and TLR5.
30946611	3	3	theme	proteins	597:604	arg1	index					513:517	disease activity index	496:517	disease activity index (DAI)	496:523	Methods: In the murine model of 3% dextran sulfate sodium (DSS)-induced colitis, we tested disease activity index (DAI), colon length, histological score and expression of tight junction (TJ) proteins (ZO-1, occludin and claudin-1), OSM, TNF-α and TLR5.
30946611	3	3	theme	proteins	597:604	arg1	OSM					638:640	OSM	638:640	OSM	638:640	Methods: In the murine model of 3% dextran sulfate sodium (DSS)-induced colitis, we tested disease activity index (DAI), colon length, histological score and expression of tight junction (TJ) proteins (ZO-1, occludin and claudin-1), OSM, TNF-α and TLR5.
30946611	3	3	theme	proteins	597:604	arg1	DAI					520:522	DAI	520:522	DAI	520:522	Methods: In the murine model of 3% dextran sulfate sodium (DSS)-induced colitis, we tested disease activity index (DAI), colon length, histological score and expression of tight junction (TJ) proteins (ZO-1, occludin and claudin-1), OSM, TNF-α and TLR5.
30946611	3	3	theme	proteins	597:604	arg1	score					553:557	histological score	540:557	histological score	540:557	Methods: In the murine model of 3% dextran sulfate sodium (DSS)-induced colitis, we tested disease activity index (DAI), colon length, histological score and expression of tight junction (TJ) proteins (ZO-1, occludin and claudin-1), OSM, TNF-α and TLR5.
30946611	3	3	theme	proteins	597:604	arg1	TNF-α					643:647	TNF-α	643:647	TNF-α	643:647	Methods: In the murine model of 3% dextran sulfate sodium (DSS)-induced colitis, we tested disease activity index (DAI), colon length, histological score and expression of tight junction (TJ) proteins (ZO-1, occludin and claudin-1), OSM, TNF-α and TLR5.
30946611	3	3	theme	proteins	597:604	arg1	expression					563:572	expression	563:572	expression	563:572	Methods: In the murine model of 3% dextran sulfate sodium (DSS)-induced colitis, we tested disease activity index (DAI), colon length, histological score and expression of tight junction (TJ) proteins (ZO-1, occludin and claudin-1), OSM, TNF-α and TLR5.
30946611	6	4	theme	DAI	1049:1051	arg1	score					1053:1057	DAI score	1049:1057	DAI score (p < .001)	1049:1068	Results: R. intestinalis in DSS-induced colitis decreased DAI score (p < .001), colon length shortening (6.46 ± 0.36 cm vs 5.65 ± 0.47 cm, p = .022), histological score (2.667 ± 1.15 vs 5.33 ± 1.14, p = .018) and increased expression of TJ proteins (p < .05).
30946611	6	4	theme	DAI	1049:1051	arg1	p < .001					1060:1067	p < .001	1060:1067	p < .001	1060:1067	Results: R. intestinalis in DSS-induced colitis decreased DAI score (p < .001), colon length shortening (6.46 ± 0.36 cm vs 5.65 ± 0.47 cm, p = .022), histological score (2.667 ± 1.15 vs 5.33 ± 1.14, p = .018) and increased expression of TJ proteins (p < .05).
30946611	4	5	theme	cellular	674:681	arg1	model					683:687	a cellular model	672:687	a cellular model	672:687	In addition, a cellular model was used to examine the role of R. intestinalis during secretion of OSM by lipopolysaccharide (LPS)-induced bone marrow-derived macrophages (BMDMs) isolated from wild-type (WT) and TLR5 knockout (TLR5 KO) mice.
30946611	10	6	theme	barrier	1768:1774	arg1	function					1776:1783	intestinal barrier function	1757:1783	intestinal barrier function	1757:1783	Conclusions: These results indicate that R. intestinalis attenuates inflammation in IBD by decreasing secretion of OSM and by promoting intestinal barrier function.
30946611	11	7	from	role	1836:1839	arg1	patients					1866:1873	patients	1866:1873	patients with IBD who are resistant to anti-TNF therapy	1866:1920	Taken together, the data provide insight into the role of the gut microbiota in patients with IBD who are resistant to anti-TNF therapy.
30946611	4	8	dep	wild-type	851:859	arg1	WT					862:863	WT	862:863	WT	862:863	In addition, a cellular model was used to examine the role of R. intestinalis during secretion of OSM by lipopolysaccharide (LPS)-induced bone marrow-derived macrophages (BMDMs) isolated from wild-type (WT) and TLR5 knockout (TLR5 KO) mice.
30946611	4	8	dep	wild-type	851:859	arg1	KO					890:891	TLR5 KO	885:891	TLR5 KO	885:891	In addition, a cellular model was used to examine the role of R. intestinalis during secretion of OSM by lipopolysaccharide (LPS)-induced bone marrow-derived macrophages (BMDMs) isolated from wild-type (WT) and TLR5 knockout (TLR5 KO) mice.
30946611	2	9	from	effects	307:313	arg1	barrier					385:391	intestinal barrier	374:391	intestinal barrier in colitis	374:402	Here, the aim was to investigate the effects of Roseburia intestinalis, a gut microbiota, on OSM and on intestinal barrier in colitis.
30946611	2	9	from	effects	307:313	arg1	OSM					363:365	OSM	363:365	OSM	363:365	Here, the aim was to investigate the effects of Roseburia intestinalis, a gut microbiota, on OSM and on intestinal barrier in colitis.
30946611	0	10	theme	experimental	111:122	arg1	colitis					124:130	acute experimental colitis	105:130	acute experimental colitis	105:130	Roseburia intestinalis inhibits oncostatin M and maintains tight junction integrity in a murine model of acute experimental colitis.
30946611	3	11	theme	-induced	468:475	arg1	colitis					477:483	3% dextran sulfate sodium (DSS)-induced colitis	437:483	3% dextran sulfate sodium (DSS)-induced colitis	437:483	Methods: In the murine model of 3% dextran sulfate sodium (DSS)-induced colitis, we tested disease activity index (DAI), colon length, histological score and expression of tight junction (TJ) proteins (ZO-1, occludin and claudin-1), OSM, TNF-α and TLR5.
30946611	8	12	theme	OSM	1434:1436	arg1	secretion					1421:1429	secretion	1421:1429	secretion of OSM (p < .05)	1421:1446	Furthermore, R. intestinalis reduced secretion of OSM (p < .05) by LPS-induced BMDMs isolated from WT and TLR5 KO mice.
30946611	4	13	theme	OSM	757:759	arg1	secretion					744:752	secretion	744:752	secretion of OSM by lipopolysaccharide (LPS)-induced bone marrow-derived macrophages (BMDMs) isolated from wild-type (WT) and TLR5 knockout (TLR5 KO) mice	744:897	In addition, a cellular model was used to examine the role of R. intestinalis during secretion of OSM by lipopolysaccharide (LPS)-induced bone marrow-derived macrophages (BMDMs) isolated from wild-type (WT) and TLR5 knockout (TLR5 KO) mice.
30946611	5	14	theme	Caco-2	977:982	arg1	cells					984:988	Caco-2 cells	977:988	Caco-2 cells	977:988	Furthermore, we evaluated the impact of OSM on expressions of TJ proteins by Caco-2 cells.
30946611	3	15	theme	activity	504:511	arg1	DAI					520:522	DAI	520:522	DAI	520:522	Methods: In the murine model of 3% dextran sulfate sodium (DSS)-induced colitis, we tested disease activity index (DAI), colon length, histological score and expression of tight junction (TJ) proteins (ZO-1, occludin and claudin-1), OSM, TNF-α and TLR5.
30946611	3	15	theme	activity	504:511	arg1	index					513:517	disease activity index	496:517	disease activity index (DAI)	496:523	Methods: In the murine model of 3% dextran sulfate sodium (DSS)-induced colitis, we tested disease activity index (DAI), colon length, histological score and expression of tight junction (TJ) proteins (ZO-1, occludin and claudin-1), OSM, TNF-α and TLR5.
30946611	6	16	theme	colon	1071:1075	arg1	shortening					1084:1093	colon length shortening	1071:1093	colon length shortening (6.46 ± 0.36 cm vs 5.65 ± 0.47 cm, p = .022)	1071:1138	Results: R. intestinalis in DSS-induced colitis decreased DAI score (p < .001), colon length shortening (6.46 ± 0.36 cm vs 5.65 ± 0.47 cm, p = .022), histological score (2.667 ± 1.15 vs 5.33 ± 1.14, p = .018) and increased expression of TJ proteins (p < .05).
30946611	4	17	theme	intestinalis	724:735	arg1	role					713:716	the role	709:716	the role of R. intestinalis during secretion of OSM by lipopolysaccharide (LPS)-induced bone marrow-derived macrophages (BMDMs) isolated from wild-type (WT) and TLR5 knockout (TLR5 KO) mice	709:897	In addition, a cellular model was used to examine the role of R. intestinalis during secretion of OSM by lipopolysaccharide (LPS)-induced bone marrow-derived macrophages (BMDMs) isolated from wild-type (WT) and TLR5 knockout (TLR5 KO) mice.
30946611	11	18	with	patients	1866:1873	arg1	IBD					1880:1882	IBD	1880:1882	IBD	1880:1882	Taken together, the data provide insight into the role of the gut microbiota in patients with IBD who are resistant to anti-TNF therapy.
30946611	4	19	theme	-induced	788:795	arg1	BMDMs					830:834	BMDMs	830:834	BMDMs	830:834	In addition, a cellular model was used to examine the role of R. intestinalis during secretion of OSM by lipopolysaccharide (LPS)-induced bone marrow-derived macrophages (BMDMs) isolated from wild-type (WT) and TLR5 knockout (TLR5 KO) mice.
30946611	4	19	theme	-induced	788:795	arg1	macrophages					817:827	lipopolysaccharide (LPS)-induced bone marrow-derived macrophages	764:827	lipopolysaccharide (LPS)-induced bone marrow-derived macrophages (BMDMs) isolated from wild-type (WT) and TLR5 knockout (TLR5 KO) mice	764:897	In addition, a cellular model was used to examine the role of R. intestinalis during secretion of OSM by lipopolysaccharide (LPS)-induced bone marrow-derived macrophages (BMDMs) isolated from wild-type (WT) and TLR5 knockout (TLR5 KO) mice.
30946611	0	20	theme	junction	65:72	arg1	integrity					74:82	tight junction integrity	59:82	tight junction integrity in a murine model of acute experimental colitis	59:130	Roseburia intestinalis inhibits oncostatin M and maintains tight junction integrity in a murine model of acute experimental colitis.
30946611	7	21	theme	OSM	1302:1304	arg1	TNF-α					1320:1324	TNF-α	1320:1324	TNF-α (p < .05)	1320:1334	In addition, R. intestinalis reduced expression of OSM (p < .05) and TNF-α (p < .05), while increasing expression of TLR5 (p < .05).
30946611	7	21	theme	OSM	1302:1304	arg1	expression					1288:1297	expression	1288:1297	expression of OSM (p < .05)	1288:1314	In addition, R. intestinalis reduced expression of OSM (p < .05) and TNF-α (p < .05), while increasing expression of TLR5 (p < .05).
30946611	7	21	theme	OSM	1302:1304	arg1	p < .05					1327:1333	p < .05	1327:1333	p < .05	1327:1333	In addition, R. intestinalis reduced expression of OSM (p < .05) and TNF-α (p < .05), while increasing expression of TLR5 (p < .05).
30946611	4	22	theme	marrow-derived	802:815	arg1	BMDMs					830:834	BMDMs	830:834	BMDMs	830:834	In addition, a cellular model was used to examine the role of R. intestinalis during secretion of OSM by lipopolysaccharide (LPS)-induced bone marrow-derived macrophages (BMDMs) isolated from wild-type (WT) and TLR5 knockout (TLR5 KO) mice.
30946611	4	22	theme	marrow-derived	802:815	arg1	macrophages					817:827	lipopolysaccharide (LPS)-induced bone marrow-derived macrophages	764:827	lipopolysaccharide (LPS)-induced bone marrow-derived macrophages (BMDMs) isolated from wild-type (WT) and TLR5 knockout (TLR5 KO) mice	764:897	In addition, a cellular model was used to examine the role of R. intestinalis during secretion of OSM by lipopolysaccharide (LPS)-induced bone marrow-derived macrophages (BMDMs) isolated from wild-type (WT) and TLR5 knockout (TLR5 KO) mice.
30946611	4	23	used	used	693:696	arg2	model					683:687	a cellular model	672:687	a cellular model	672:687	In addition, a cellular model was used to examine the role of R. intestinalis during secretion of OSM by lipopolysaccharide (LPS)-induced bone marrow-derived macrophages (BMDMs) isolated from wild-type (WT) and TLR5 knockout (TLR5 KO) mice.
30946611	1	24	theme	oncostatin	154:163	arg1	OSM					168:170	OSM	168:170	OSM	168:170	Objective: Levels of oncostatin M (OSM) and the composition of gut microbiota predict responses to anti-TNF agents used for IBD therapy.
30946611	1	24	theme	oncostatin	154:163	arg1	M					165:165	oncostatin M	154:165	oncostatin M (OSM)	154:171	Objective: Levels of oncostatin M (OSM) and the composition of gut microbiota predict responses to anti-TNF agents used for IBD therapy.
30946611	2	25	from	barrier	385:391	arg1	colitis					396:402	colitis	396:402	colitis	396:402	Here, the aim was to investigate the effects of Roseburia intestinalis, a gut microbiota, on OSM and on intestinal barrier in colitis.
30946611	0	26	theme	murine	89:94	arg1	model					96:100	a murine model	87:100	a murine model of acute experimental colitis	87:130	Roseburia intestinalis inhibits oncostatin M and maintains tight junction integrity in a murine model of acute experimental colitis.
30946611	8	27	theme	KO	1495:1496	arg1	mice					1498:1501	WT and TLR5 KO mice	1483:1501	WT and TLR5 KO mice	1483:1501	Furthermore, R. intestinalis reduced secretion of OSM (p < .05) by LPS-induced BMDMs isolated from WT and TLR5 KO mice.
30946611	0	28	theme	Roseburia	0:8	arg1	intestinalis					10:21	Roseburia intestinalis	0:21	Roseburia intestinalis	0:21	Roseburia intestinalis inhibits oncostatin M and maintains tight junction integrity in a murine model of acute experimental colitis.
30946611	2	29	theme	intestinal	374:383	arg1	barrier					385:391	intestinal barrier	374:391	intestinal barrier in colitis	374:402	Here, the aim was to investigate the effects of Roseburia intestinalis, a gut microbiota, on OSM and on intestinal barrier in colitis.
30946611	4	30	link	marrow-derived	802:815	arg1	BMDMs					830:834	BMDMs	830:834	BMDMs	830:834	In addition, a cellular model was used to examine the role of R. intestinalis during secretion of OSM by lipopolysaccharide (LPS)-induced bone marrow-derived macrophages (BMDMs) isolated from wild-type (WT) and TLR5 knockout (TLR5 KO) mice.
30946611	4	30	link	marrow-derived	802:815	arg1	macrophages					817:827	lipopolysaccharide (LPS)-induced bone marrow-derived macrophages	764:827	lipopolysaccharide (LPS)-induced bone marrow-derived macrophages (BMDMs) isolated from wild-type (WT) and TLR5 knockout (TLR5 KO) mice	764:897	In addition, a cellular model was used to examine the role of R. intestinalis during secretion of OSM by lipopolysaccharide (LPS)-induced bone marrow-derived macrophages (BMDMs) isolated from wild-type (WT) and TLR5 knockout (TLR5 KO) mice.
30946611	5	31	theme	TJ	962:963	arg1	proteins					965:972	TJ proteins	962:972	TJ proteins	962:972	Furthermore, we evaluated the impact of OSM on expressions of TJ proteins by Caco-2 cells.
30946611	11	32	theme	microbiota	1852:1861	arg1	role					1836:1839	the role	1832:1839	the role of the gut microbiota in patients with IBD who are resistant to anti-TNF therapy	1832:1920	Taken together, the data provide insight into the role of the gut microbiota in patients with IBD who are resistant to anti-TNF therapy.
30946611	10	33	from	inflammation	1689:1700	arg1	IBD					1705:1707	IBD	1705:1707	IBD	1705:1707	Conclusions: These results indicate that R. intestinalis attenuates inflammation in IBD by decreasing secretion of OSM and by promoting intestinal barrier function.
30946611	3	34	theme	colon	526:530	arg1	length					532:537	colon length	526:537	colon length	526:537	Methods: In the murine model of 3% dextran sulfate sodium (DSS)-induced colitis, we tested disease activity index (DAI), colon length, histological score and expression of tight junction (TJ) proteins (ZO-1, occludin and claudin-1), OSM, TNF-α and TLR5.
30946611	3	35	theme	murine	421:426	arg1	model					428:432	the murine model	417:432	the murine model of 3% dextran sulfate sodium (DSS)-induced colitis	417:483	Methods: In the murine model of 3% dextran sulfate sodium (DSS)-induced colitis, we tested disease activity index (DAI), colon length, histological score and expression of tight junction (TJ) proteins (ZO-1, occludin and claudin-1), OSM, TNF-α and TLR5.
30946611	6	36	theme	histological	1141:1152	arg1	±					1182:1182	2.667 ± 1.15 vs 5.33 ± 1.14, p = .018	1161:1197	±	1182:1182	Results: R. intestinalis in DSS-induced colitis decreased DAI score (p < .001), colon length shortening (6.46 ± 0.36 cm vs 5.65 ± 0.47 cm, p = .022), histological score (2.667 ± 1.15 vs 5.33 ± 1.14, p = .018) and increased expression of TJ proteins (p < .05).
30946611	6	36	theme	histological	1141:1152	arg1	score					1154:1158	histological score	1141:1158	histological score (2.667 ± 1.15 vs 5.33 ± 1.14, p = .018)	1141:1198	Results: R. intestinalis in DSS-induced colitis decreased DAI score (p < .001), colon length shortening (6.46 ± 0.36 cm vs 5.65 ± 0.47 cm, p = .022), histological score (2.667 ± 1.15 vs 5.33 ± 1.14, p = .018) and increased expression of TJ proteins (p < .05).
30946611	5	37	theme	OSM	940:942	arg1	impact					930:935	the impact	926:935	the impact of OSM on expressions of TJ proteins	926:972	Furthermore, we evaluated the impact of OSM on expressions of TJ proteins by Caco-2 cells.
30946611	1	38	theme	gut	196:198	arg1	microbiota					200:209	gut microbiota	196:209	gut microbiota	196:209	Objective: Levels of oncostatin M (OSM) and the composition of gut microbiota predict responses to anti-TNF agents used for IBD therapy.
30946611	10	39	theme	OSM	1736:1738	arg1	secretion					1723:1731	secretion	1723:1731	secretion of OSM	1723:1738	Conclusions: These results indicate that R. intestinalis attenuates inflammation in IBD by decreasing secretion of OSM and by promoting intestinal barrier function.
30946611	6	40	theme	5.33	1177:1180	arg1	±					1182:1182	2.667 ± 1.15 vs 5.33 ± 1.14, p = .018	1161:1197	±	1182:1182	Results: R. intestinalis in DSS-induced colitis decreased DAI score (p < .001), colon length shortening (6.46 ± 0.36 cm vs 5.65 ± 0.47 cm, p = .022), histological score (2.667 ± 1.15 vs 5.33 ± 1.14, p = .018) and increased expression of TJ proteins (p < .05).
30946611	6	40	theme	5.33	1177:1180	arg1	score					1154:1158	histological score	1141:1158	histological score (2.667 ± 1.15 vs 5.33 ± 1.14, p = .018)	1141:1198	Results: R. intestinalis in DSS-induced colitis decreased DAI score (p < .001), colon length shortening (6.46 ± 0.36 cm vs 5.65 ± 0.47 cm, p = .022), histological score (2.667 ± 1.15 vs 5.33 ± 1.14, p = .018) and increased expression of TJ proteins (p < .05).
30946611	3	41	theme	%	438:438	arg1	colitis					477:483	3% dextran sulfate sodium (DSS)-induced colitis	437:483	3% dextran sulfate sodium (DSS)-induced colitis	437:483	Methods: In the murine model of 3% dextran sulfate sodium (DSS)-induced colitis, we tested disease activity index (DAI), colon length, histological score and expression of tight junction (TJ) proteins (ZO-1, occludin and claudin-1), OSM, TNF-α and TLR5.
30946611	6	42	theme	increased	1204:1212	arg1	expression					1214:1223	increased expression	1204:1223	increased expression of TJ proteins (p < .05)	1204:1248	Results: R. intestinalis in DSS-induced colitis decreased DAI score (p < .001), colon length shortening (6.46 ± 0.36 cm vs 5.65 ± 0.47 cm, p = .022), histological score (2.667 ± 1.15 vs 5.33 ± 1.14, p = .018) and increased expression of TJ proteins (p < .05).
30946611	9	43	theme	TJ	1546:1547	arg1	proteins					1549:1556	TJ proteins	1546:1556	TJ proteins (p < .05)	1546:1566	Moreover, OSM downregulated expression of TJ proteins (p < .05) by Caco-2 cells in a concentration-dependent manner.
30946611	9	43	theme	TJ	1546:1547	arg1	p < .05					1559:1565	p < .05	1559:1565	p < .05	1559:1565	Moreover, OSM downregulated expression of TJ proteins (p < .05) by Caco-2 cells in a concentration-dependent manner.
30946611	4	44	attach	isolated	837:844	arg2	macrophages					817:827	lipopolysaccharide (LPS)-induced bone marrow-derived macrophages	764:827	lipopolysaccharide (LPS)-induced bone marrow-derived macrophages (BMDMs) isolated from wild-type (WT) and TLR5 knockout (TLR5 KO) mice	764:897	In addition, a cellular model was used to examine the role of R. intestinalis during secretion of OSM by lipopolysaccharide (LPS)-induced bone marrow-derived macrophages (BMDMs) isolated from wild-type (WT) and TLR5 knockout (TLR5 KO) mice.
30946611	4	44	attach	isolated	837:844	arg1	mice					894:897	wild-type (WT) and TLR5 knockout (TLR5 KO) mice	851:897	wild-type (WT) and TLR5 knockout (TLR5 KO) mice	851:897	In addition, a cellular model was used to examine the role of R. intestinalis during secretion of OSM by lipopolysaccharide (LPS)-induced bone marrow-derived macrophages (BMDMs) isolated from wild-type (WT) and TLR5 knockout (TLR5 KO) mice.
30946611	4	44	attach	isolated	837:844	arg2	BMDMs					830:834	BMDMs	830:834	BMDMs	830:834	In addition, a cellular model was used to examine the role of R. intestinalis during secretion of OSM by lipopolysaccharide (LPS)-induced bone marrow-derived macrophages (BMDMs) isolated from wild-type (WT) and TLR5 knockout (TLR5 KO) mice.
30946611	10	45	theme	intestinal	1757:1766	arg1	function					1776:1783	intestinal barrier function	1757:1783	intestinal barrier function	1757:1783	Conclusions: These results indicate that R. intestinalis attenuates inflammation in IBD by decreasing secretion of OSM and by promoting intestinal barrier function.
30946611	3	46	theme	tight	577:581	arg1	junction					583:590	tight junction	577:590	tight junction (TJ) proteins (ZO-1, occludin and claudin-1)	577:635	Methods: In the murine model of 3% dextran sulfate sodium (DSS)-induced colitis, we tested disease activity index (DAI), colon length, histological score and expression of tight junction (TJ) proteins (ZO-1, occludin and claudin-1), OSM, TNF-α and TLR5.
30946611	3	46	theme	tight	577:581	arg1	TJ					593:594	TJ	593:594	TJ	593:594	Methods: In the murine model of 3% dextran sulfate sodium (DSS)-induced colitis, we tested disease activity index (DAI), colon length, histological score and expression of tight junction (TJ) proteins (ZO-1, occludin and claudin-1), OSM, TNF-α and TLR5.
30946611	5	47	from	impact	930:935	arg1	expressions					947:957	expressions	947:957	expressions of TJ proteins	947:972	Furthermore, we evaluated the impact of OSM on expressions of TJ proteins by Caco-2 cells.
30946611	1	48	theme	M	165:165	arg1	Levels					144:149	Levels	144:149	Levels of oncostatin M (OSM)	144:171	Objective: Levels of oncostatin M (OSM) and the composition of gut microbiota predict responses to anti-TNF agents used for IBD therapy.
30946611	1	48	theme	M	165:165	arg1	composition					181:191	the composition	177:191	the composition of gut microbiota	177:209	Objective: Levels of oncostatin M (OSM) and the composition of gut microbiota predict responses to anti-TNF agents used for IBD therapy.
30946611	9	49	theme	Caco-2	1571:1576	arg1	cells					1578:1582	Caco-2 cells	1571:1582	Caco-2 cells	1571:1582	Moreover, OSM downregulated expression of TJ proteins (p < .05) by Caco-2 cells in a concentration-dependent manner.
30946611	3	50	dep	proteins	597:604	arg1	occludin					613:620	occludin	613:620	occludin	613:620	Methods: In the murine model of 3% dextran sulfate sodium (DSS)-induced colitis, we tested disease activity index (DAI), colon length, histological score and expression of tight junction (TJ) proteins (ZO-1, occludin and claudin-1), OSM, TNF-α and TLR5.
30946611	3	50	dep	proteins	597:604	arg1	ZO-1					607:610	ZO-1	607:610	ZO-1	607:610	Methods: In the murine model of 3% dextran sulfate sodium (DSS)-induced colitis, we tested disease activity index (DAI), colon length, histological score and expression of tight junction (TJ) proteins (ZO-1, occludin and claudin-1), OSM, TNF-α and TLR5.
30946611	3	50	dep	proteins	597:604	arg1	proteins					597:604	tight junction (TJ) proteins	577:604	tight junction (TJ) proteins (ZO-1, occludin and claudin-1)	577:635	Methods: In the murine model of 3% dextran sulfate sodium (DSS)-induced colitis, we tested disease activity index (DAI), colon length, histological score and expression of tight junction (TJ) proteins (ZO-1, occludin and claudin-1), OSM, TNF-α and TLR5.
30946611	3	50	dep	proteins	597:604	arg1	claudin-1					626:634	claudin-1	626:634	claudin-1	626:634	Methods: In the murine model of 3% dextran sulfate sodium (DSS)-induced colitis, we tested disease activity index (DAI), colon length, histological score and expression of tight junction (TJ) proteins (ZO-1, occludin and claudin-1), OSM, TNF-α and TLR5.
30946611	6	51	theme	DSS-induced	1019:1029	arg1	colitis					1031:1037	DSS-induced colitis	1019:1037	DSS-induced colitis	1019:1037	Results: R. intestinalis in DSS-induced colitis decreased DAI score (p < .001), colon length shortening (6.46 ± 0.36 cm vs 5.65 ± 0.47 cm, p = .022), histological score (2.667 ± 1.15 vs 5.33 ± 1.14, p = .018) and increased expression of TJ proteins (p < .05).
30946611	3	52	theme	colitis	477:483	arg1	model					428:432	the murine model	417:432	the murine model of 3% dextran sulfate sodium (DSS)-induced colitis	417:483	Methods: In the murine model of 3% dextran sulfate sodium (DSS)-induced colitis, we tested disease activity index (DAI), colon length, histological score and expression of tight junction (TJ) proteins (ZO-1, occludin and claudin-1), OSM, TNF-α and TLR5.
30946611	8	53	theme	R.	1397:1398	arg1	intestinalis					1400:1411	R. intestinalis	1397:1411	R. intestinalis	1397:1411	Furthermore, R. intestinalis reduced secretion of OSM (p < .05) by LPS-induced BMDMs isolated from WT and TLR5 KO mice.
30946611	0	54	theme	colitis	124:130	arg1	model					96:100	a murine model	87:100	a murine model of acute experimental colitis	87:130	Roseburia intestinalis inhibits oncostatin M and maintains tight junction integrity in a murine model of acute experimental colitis.
30946611	9	55	theme	concentration-dependent	1589:1611	arg1	manner					1613:1618	a concentration-dependent manner	1587:1618	a concentration-dependent manner	1587:1618	Moreover, OSM downregulated expression of TJ proteins (p < .05) by Caco-2 cells in a concentration-dependent manner.
30946611	3	56	theme	disease	496:502	arg1	DAI					520:522	DAI	520:522	DAI	520:522	Methods: In the murine model of 3% dextran sulfate sodium (DSS)-induced colitis, we tested disease activity index (DAI), colon length, histological score and expression of tight junction (TJ) proteins (ZO-1, occludin and claudin-1), OSM, TNF-α and TLR5.
30946611	3	56	theme	disease	496:502	arg1	index					513:517	disease activity index	496:517	disease activity index (DAI)	496:523	Methods: In the murine model of 3% dextran sulfate sodium (DSS)-induced colitis, we tested disease activity index (DAI), colon length, histological score and expression of tight junction (TJ) proteins (ZO-1, occludin and claudin-1), OSM, TNF-α and TLR5.
30946611	6	57	theme	proteins	1231:1238	arg1	±					1182:1182	2.667 ± 1.15 vs 5.33 ± 1.14, p = .018	1161:1197	±	1182:1182	Results: R. intestinalis in DSS-induced colitis decreased DAI score (p < .001), colon length shortening (6.46 ± 0.36 cm vs 5.65 ± 0.47 cm, p = .022), histological score (2.667 ± 1.15 vs 5.33 ± 1.14, p = .018) and increased expression of TJ proteins (p < .05).
30946611	6	57	theme	proteins	1231:1238	arg1	expression					1214:1223	increased expression	1204:1223	increased expression of TJ proteins (p < .05)	1204:1248	Results: R. intestinalis in DSS-induced colitis decreased DAI score (p < .001), colon length shortening (6.46 ± 0.36 cm vs 5.65 ± 0.47 cm, p = .022), histological score (2.667 ± 1.15 vs 5.33 ± 1.14, p = .018) and increased expression of TJ proteins (p < .05).
30946611	6	57	theme	proteins	1231:1238	arg1	score					1053:1057	DAI score	1049:1057	DAI score (p < .001)	1049:1068	Results: R. intestinalis in DSS-induced colitis decreased DAI score (p < .001), colon length shortening (6.46 ± 0.36 cm vs 5.65 ± 0.47 cm, p = .022), histological score (2.667 ± 1.15 vs 5.33 ± 1.14, p = .018) and increased expression of TJ proteins (p < .05).
30946611	6	57	theme	proteins	1231:1238	arg1	score					1154:1158	histological score	1141:1158	histological score (2.667 ± 1.15 vs 5.33 ± 1.14, p = .018)	1141:1198	Results: R. intestinalis in DSS-induced colitis decreased DAI score (p < .001), colon length shortening (6.46 ± 0.36 cm vs 5.65 ± 0.47 cm, p = .022), histological score (2.667 ± 1.15 vs 5.33 ± 1.14, p = .018) and increased expression of TJ proteins (p < .05).
30946611	6	57	theme	proteins	1231:1238	arg1	shortening					1084:1093	colon length shortening	1071:1093	colon length shortening (6.46 ± 0.36 cm vs 5.65 ± 0.47 cm, p = .022)	1071:1138	Results: R. intestinalis in DSS-induced colitis decreased DAI score (p < .001), colon length shortening (6.46 ± 0.36 cm vs 5.65 ± 0.47 cm, p = .022), histological score (2.667 ± 1.15 vs 5.33 ± 1.14, p = .018) and increased expression of TJ proteins (p < .05).
30946611	6	57	theme	proteins	1231:1238	arg1	p < .001					1060:1067	p < .001	1060:1067	p < .001	1060:1067	Results: R. intestinalis in DSS-induced colitis decreased DAI score (p < .001), colon length shortening (6.46 ± 0.36 cm vs 5.65 ± 0.47 cm, p = .022), histological score (2.667 ± 1.15 vs 5.33 ± 1.14, p = .018) and increased expression of TJ proteins (p < .05).
30946611	6	58	dep	shortening	1084:1093	arg1	p = .022					1130:1137	p = .022	1130:1137	p = .022	1130:1137	Results: R. intestinalis in DSS-induced colitis decreased DAI score (p < .001), colon length shortening (6.46 ± 0.36 cm vs 5.65 ± 0.47 cm, p = .022), histological score (2.667 ± 1.15 vs 5.33 ± 1.14, p = .018) and increased expression of TJ proteins (p < .05).
30946611	6	58	dep	shortening	1084:1093	arg1	cm					1126:1127	6.46 ± 0.36 cm vs 5.65 ± 0.47 cm	1096:1127	6.46 ± 0.36 cm vs 5.65 ± 0.47 cm	1096:1127	Results: R. intestinalis in DSS-induced colitis decreased DAI score (p < .001), colon length shortening (6.46 ± 0.36 cm vs 5.65 ± 0.47 cm, p = .022), histological score (2.667 ± 1.15 vs 5.33 ± 1.14, p = .018) and increased expression of TJ proteins (p < .05).
30946611	0	59	from	integrity	74:82	arg1	model					96:100	a murine model	87:100	a murine model of acute experimental colitis	87:130	Roseburia intestinalis inhibits oncostatin M and maintains tight junction integrity in a murine model of acute experimental colitis.
30946611	6	60	theme	R.	1000:1001	arg1	intestinalis					1003:1014	R. intestinalis	1000:1014	R. intestinalis in DSS-induced colitis	1000:1037	Results: R. intestinalis in DSS-induced colitis decreased DAI score (p < .001), colon length shortening (6.46 ± 0.36 cm vs 5.65 ± 0.47 cm, p = .022), histological score (2.667 ± 1.15 vs 5.33 ± 1.14, p = .018) and increased expression of TJ proteins (p < .05).
30946611	6	61	theme	length	1077:1082	arg1	shortening					1084:1093	colon length shortening	1071:1093	colon length shortening (6.46 ± 0.36 cm vs 5.65 ± 0.47 cm, p = .022)	1071:1138	Results: R. intestinalis in DSS-induced colitis decreased DAI score (p < .001), colon length shortening (6.46 ± 0.36 cm vs 5.65 ± 0.47 cm, p = .022), histological score (2.667 ± 1.15 vs 5.33 ± 1.14, p = .018) and increased expression of TJ proteins (p < .05).
30946611	10	62	dep	attenuates	1678:1687	arg1	decreasing					1712:1721	decreasing	1712:1721	decreasing secretion of OSM	1712:1738	Conclusions: These results indicate that R. intestinalis attenuates inflammation in IBD by decreasing secretion of OSM and by promoting intestinal barrier function.
30946611	10	62	dep	attenuates	1678:1687	arg1	promoting					1747:1755	promoting	1747:1755	promoting intestinal barrier function	1747:1783	Conclusions: These results indicate that R. intestinalis attenuates inflammation in IBD by decreasing secretion of OSM and by promoting intestinal barrier function.
30946611	4	63	theme	R.	721:722	arg1	intestinalis					724:735	R. intestinalis	721:735	R. intestinalis	721:735	In addition, a cellular model was used to examine the role of R. intestinalis during secretion of OSM by lipopolysaccharide (LPS)-induced bone marrow-derived macrophages (BMDMs) isolated from wild-type (WT) and TLR5 knockout (TLR5 KO) mice.
30946611	6	64	from	intestinalis	1003:1014	arg1	colitis					1031:1037	DSS-induced colitis	1019:1037	DSS-induced colitis	1019:1037	Results: R. intestinalis in DSS-induced colitis decreased DAI score (p < .001), colon length shortening (6.46 ± 0.36 cm vs 5.65 ± 0.47 cm, p = .022), histological score (2.667 ± 1.15 vs 5.33 ± 1.14, p = .018) and increased expression of TJ proteins (p < .05).
30946611	8	65	theme	LPS-induced	1451:1461	arg1	BMDMs					1463:1467	LPS-induced BMDMs	1451:1467	LPS-induced BMDMs isolated from WT and TLR5 KO mice	1451:1501	Furthermore, R. intestinalis reduced secretion of OSM (p < .05) by LPS-induced BMDMs isolated from WT and TLR5 KO mice.
30946611	4	66	theme	bone	797:800	arg1	BMDMs					830:834	BMDMs	830:834	BMDMs	830:834	In addition, a cellular model was used to examine the role of R. intestinalis during secretion of OSM by lipopolysaccharide (LPS)-induced bone marrow-derived macrophages (BMDMs) isolated from wild-type (WT) and TLR5 knockout (TLR5 KO) mice.
30946611	4	66	theme	bone	797:800	arg1	macrophages					817:827	lipopolysaccharide (LPS)-induced bone marrow-derived macrophages	764:827	lipopolysaccharide (LPS)-induced bone marrow-derived macrophages (BMDMs) isolated from wild-type (WT) and TLR5 knockout (TLR5 KO) mice	764:897	In addition, a cellular model was used to examine the role of R. intestinalis during secretion of OSM by lipopolysaccharide (LPS)-induced bone marrow-derived macrophages (BMDMs) isolated from wild-type (WT) and TLR5 knockout (TLR5 KO) mice.
30946611	10	67	dep	R.	1662:1663	arg1	intestinalis					1665:1676	R. intestinalis	1662:1676	R. intestinalis	1662:1676	Conclusions: These results indicate that R. intestinalis attenuates inflammation in IBD by decreasing secretion of OSM and by promoting intestinal barrier function.
30946611	8	68	theme	WT	1483:1484	arg1	mice					1498:1501	WT and TLR5 KO mice	1483:1501	WT and TLR5 KO mice	1483:1501	Furthermore, R. intestinalis reduced secretion of OSM (p < .05) by LPS-induced BMDMs isolated from WT and TLR5 KO mice.
30946611	7	69	theme	R.	1264:1265	arg1	intestinalis					1267:1278	R. intestinalis	1264:1278	R. intestinalis	1264:1278	In addition, R. intestinalis reduced expression of OSM (p < .05) and TNF-α (p < .05), while increasing expression of TLR5 (p < .05).
30946611	11	70	theme	anti-TNF	1905:1912	arg1	therapy					1914:1920	anti-TNF therapy	1905:1920	anti-TNF therapy	1905:1920	Taken together, the data provide insight into the role of the gut microbiota in patients with IBD who are resistant to anti-TNF therapy.
30946611	0	71	theme	acute	105:109	arg1	colitis					124:130	acute experimental colitis	105:130	acute experimental colitis	105:130	Roseburia intestinalis inhibits oncostatin M and maintains tight junction integrity in a murine model of acute experimental colitis.
30946611	8	72	theme	TLR5	1490:1493	arg1	mice					1498:1501	WT and TLR5 KO mice	1483:1501	WT and TLR5 KO mice	1483:1501	Furthermore, R. intestinalis reduced secretion of OSM (p < .05) by LPS-induced BMDMs isolated from WT and TLR5 KO mice.
30946611	2	73	theme	gut	344:346	arg1	microbiota					348:357	a gut microbiota	342:357	a gut microbiota	342:357	Here, the aim was to investigate the effects of Roseburia intestinalis, a gut microbiota, on OSM and on intestinal barrier in colitis.
30946611	2	73	theme	gut	344:346	arg1	intestinalis					328:339	Roseburia intestinalis	318:339	Roseburia intestinalis	318:339	Here, the aim was to investigate the effects of Roseburia intestinalis, a gut microbiota, on OSM and on intestinal barrier in colitis.
30946611	0	74	theme	oncostatin	32:41	arg1	M					43:43	oncostatin M	32:43	oncostatin M	32:43	Roseburia intestinalis inhibits oncostatin M and maintains tight junction integrity in a murine model of acute experimental colitis.
30946611	1	75	theme	IBD	257:259	arg1	therapy					261:267	IBD therapy	257:267	IBD therapy	257:267	Objective: Levels of oncostatin M (OSM) and the composition of gut microbiota predict responses to anti-TNF agents used for IBD therapy.
30946611	7	76	theme	TLR5	1368:1371	arg1	expression					1354:1363	expression	1354:1363	expression of TLR5 (p < .05)	1354:1381	In addition, R. intestinalis reduced expression of OSM (p < .05) and TNF-α (p < .05), while increasing expression of TLR5 (p < .05).
30946611	4	77	theme	wild-type	851:859	arg1	mice					894:897	wild-type (WT) and TLR5 knockout (TLR5 KO) mice	851:897	wild-type (WT) and TLR5 knockout (TLR5 KO) mice	851:897	In addition, a cellular model was used to examine the role of R. intestinalis during secretion of OSM by lipopolysaccharide (LPS)-induced bone marrow-derived macrophages (BMDMs) isolated from wild-type (WT) and TLR5 knockout (TLR5 KO) mice.
30946611	2	78	theme	intestinalis	328:339	arg1	effects					307:313	the effects	303:313	the effects of Roseburia intestinalis, a gut microbiota, on OSM and on intestinal barrier in colitis	303:402	Here, the aim was to investigate the effects of Roseburia intestinalis, a gut microbiota, on OSM and on intestinal barrier in colitis.
30946611	6	79	theme	2.667 ± 1.15	1161:1172	arg1	±					1182:1182	2.667 ± 1.15 vs 5.33 ± 1.14, p = .018	1161:1197	±	1182:1182	Results: R. intestinalis in DSS-induced colitis decreased DAI score (p < .001), colon length shortening (6.46 ± 0.36 cm vs 5.65 ± 0.47 cm, p = .022), histological score (2.667 ± 1.15 vs 5.33 ± 1.14, p = .018) and increased expression of TJ proteins (p < .05).
30946611	6	79	theme	2.667 ± 1.15	1161:1172	arg1	score					1154:1158	histological score	1141:1158	histological score (2.667 ± 1.15 vs 5.33 ± 1.14, p = .018)	1141:1198	Results: R. intestinalis in DSS-induced colitis decreased DAI score (p < .001), colon length shortening (6.46 ± 0.36 cm vs 5.65 ± 0.47 cm, p = .022), histological score (2.667 ± 1.15 vs 5.33 ± 1.14, p = .018) and increased expression of TJ proteins (p < .05).
30946611	5	80	theme	proteins	965:972	arg1	expressions					947:957	expressions	947:957	expressions of TJ proteins	947:972	Furthermore, we evaluated the impact of OSM on expressions of TJ proteins by Caco-2 cells.
30946611	0	81	theme	tight	59:63	arg1	integrity					74:82	tight junction integrity	59:82	tight junction integrity in a murine model of acute experimental colitis	59:130	Roseburia intestinalis inhibits oncostatin M and maintains tight junction integrity in a murine model of acute experimental colitis.
30946611	11	82	theme	gut	1848:1850	arg1	microbiota					1852:1861	the gut microbiota	1844:1861	the gut microbiota	1844:1861	Taken together, the data provide insight into the role of the gut microbiota in patients with IBD who are resistant to anti-TNF therapy.
30946611	8	83	attach	isolated	1469:1476	arg2	BMDMs					1463:1467	LPS-induced BMDMs	1451:1467	LPS-induced BMDMs isolated from WT and TLR5 KO mice	1451:1501	Furthermore, R. intestinalis reduced secretion of OSM (p < .05) by LPS-induced BMDMs isolated from WT and TLR5 KO mice.
30946611	8	83	attach	isolated	1469:1476	arg1	mice					1498:1501	WT and TLR5 KO mice	1483:1501	WT and TLR5 KO mice	1483:1501	Furthermore, R. intestinalis reduced secretion of OSM (p < .05) by LPS-induced BMDMs isolated from WT and TLR5 KO mice.
30946611	6	84	theme	TJ	1228:1229	arg1	p < .05					1241:1247	p < .05	1241:1247	p < .05	1241:1247	Results: R. intestinalis in DSS-induced colitis decreased DAI score (p < .001), colon length shortening (6.46 ± 0.36 cm vs 5.65 ± 0.47 cm, p = .022), histological score (2.667 ± 1.15 vs 5.33 ± 1.14, p = .018) and increased expression of TJ proteins (p < .05).
30946611	6	84	theme	TJ	1228:1229	arg1	proteins					1231:1238	TJ proteins	1228:1238	TJ proteins (p < .05)	1228:1248	Results: R. intestinalis in DSS-induced colitis decreased DAI score (p < .001), colon length shortening (6.46 ± 0.36 cm vs 5.65 ± 0.47 cm, p = .022), histological score (2.667 ± 1.15 vs 5.33 ± 1.14, p = .018) and increased expression of TJ proteins (p < .05).
30946611	4	85	theme	knockout	875:882	arg1	mice					894:897	wild-type (WT) and TLR5 knockout (TLR5 KO) mice	851:897	wild-type (WT) and TLR5 knockout (TLR5 KO) mice	851:897	In addition, a cellular model was used to examine the role of R. intestinalis during secretion of OSM by lipopolysaccharide (LPS)-induced bone marrow-derived macrophages (BMDMs) isolated from wild-type (WT) and TLR5 knockout (TLR5 KO) mice.
30946611	3	86	theme	histological	540:551	arg1	score					553:557	histological score	540:557	histological score	540:557	Methods: In the murine model of 3% dextran sulfate sodium (DSS)-induced colitis, we tested disease activity index (DAI), colon length, histological score and expression of tight junction (TJ) proteins (ZO-1, occludin and claudin-1), OSM, TNF-α and TLR5.
30946611	1	87	theme	microbiota	200:209	arg1	Levels					144:149	Levels	144:149	Levels of oncostatin M (OSM)	144:171	Objective: Levels of oncostatin M (OSM) and the composition of gut microbiota predict responses to anti-TNF agents used for IBD therapy.
30946611	1	87	theme	microbiota	200:209	arg1	composition					181:191	the composition	177:191	the composition of gut microbiota	177:209	Objective: Levels of oncostatin M (OSM) and the composition of gut microbiota predict responses to anti-TNF agents used for IBD therapy.
30946611	4	88	theme	TLR5	885:888	arg1	KO					890:891	TLR5 KO	885:891	TLR5 KO	885:891	In addition, a cellular model was used to examine the role of R. intestinalis during secretion of OSM by lipopolysaccharide (LPS)-induced bone marrow-derived macrophages (BMDMs) isolated from wild-type (WT) and TLR5 knockout (TLR5 KO) mice.
30946611	3	89	theme	junction	583:590	arg1	occludin					613:620	occludin	613:620	occludin	613:620	Methods: In the murine model of 3% dextran sulfate sodium (DSS)-induced colitis, we tested disease activity index (DAI), colon length, histological score and expression of tight junction (TJ) proteins (ZO-1, occludin and claudin-1), OSM, TNF-α and TLR5.
30946611	3	89	theme	junction	583:590	arg1	ZO-1					607:610	ZO-1	607:610	ZO-1	607:610	Methods: In the murine model of 3% dextran sulfate sodium (DSS)-induced colitis, we tested disease activity index (DAI), colon length, histological score and expression of tight junction (TJ) proteins (ZO-1, occludin and claudin-1), OSM, TNF-α and TLR5.
30946611	3	89	theme	junction	583:590	arg1	proteins					597:604	tight junction (TJ) proteins	577:604	tight junction (TJ) proteins (ZO-1, occludin and claudin-1)	577:635	Methods: In the murine model of 3% dextran sulfate sodium (DSS)-induced colitis, we tested disease activity index (DAI), colon length, histological score and expression of tight junction (TJ) proteins (ZO-1, occludin and claudin-1), OSM, TNF-α and TLR5.
30946611	3	89	theme	junction	583:590	arg1	claudin-1					626:634	claudin-1	626:634	claudin-1	626:634	Methods: In the murine model of 3% dextran sulfate sodium (DSS)-induced colitis, we tested disease activity index (DAI), colon length, histological score and expression of tight junction (TJ) proteins (ZO-1, occludin and claudin-1), OSM, TNF-α and TLR5.
30946611	2	90	theme	Roseburia	318:326	arg1	microbiota					348:357	a gut microbiota	342:357	a gut microbiota	342:357	Here, the aim was to investigate the effects of Roseburia intestinalis, a gut microbiota, on OSM and on intestinal barrier in colitis.
30946611	2	90	theme	Roseburia	318:326	arg1	intestinalis					328:339	Roseburia intestinalis	318:339	Roseburia intestinalis	318:339	Here, the aim was to investigate the effects of Roseburia intestinalis, a gut microbiota, on OSM and on intestinal barrier in colitis.
30489236	5	0	dep	rRNA	434:437	arg1	the					417:419	the	417:419	the	417:419	On the basis of 16S rRNA gene sequence similarities and phylogenetic analysis, strain SYSU K10002T was most closely related to the type strains of Nocardiaaltamirensis NBRC 108246T (99.0 % sequence similarity) and Nocardiatenerifensis NBRC 101015T (98.8 %) and is therefore considered to represent a member of the genus Nocardia.
30489236	5	0	dep	rRNA	434:437	arg1	basis					421:425	basis	421:425	basis	421:425	On the basis of 16S rRNA gene sequence similarities and phylogenetic analysis, strain SYSU K10002T was most closely related to the type strains of Nocardiaaltamirensis NBRC 108246T (99.0 % sequence similarity) and Nocardiatenerifensis NBRC 101015T (98.8 %) and is therefore considered to represent a member of the genus Nocardia.
30489236	5	0	dep	rRNA	434:437	arg1	similarities					453:464	gene sequence similarities	439:464	gene sequence similarities	439:464	On the basis of 16S rRNA gene sequence similarities and phylogenetic analysis, strain SYSU K10002T was most closely related to the type strains of Nocardiaaltamirensis NBRC 108246T (99.0 % sequence similarity) and Nocardiatenerifensis NBRC 101015T (98.8 %) and is therefore considered to represent a member of the genus Nocardia.
30489236	15	1	theme	type	1623:1626	arg1	strain					1628:1633	The type strain	1619:1633	The type strain	1619:1633	The type strain is SYSU K10002T (=KCTC 39849T=DSM 103986T).
30489236	15	1	theme	type	1623:1626	arg1	K10002T					1643:1649	SYSU K10002T	1638:1649	SYSU K10002T (=KCTC 39849T=DSM 103986T)	1638:1676	The type strain is SYSU K10002T (=KCTC 39849T=DSM 103986T).
30489236	13	2	theme	name	1578:1581	arg1	sp					1597:1598	the name Nocardiaaurea sp	1574:1598	the name Nocardiaaurea sp	1574:1598	On the basis of phenotypic, genotypic and phylogenetic data, strain SYSU K10002T represents a novel species of the genus Nocardia, for which the name Nocardiaaurea sp.
30489236	10	3	theme	polar	1181:1185	arg1	lipids					1187:1192	The polar lipids	1177:1192	The polar lipids	1177:1192	The polar lipids contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and an unidentified glycolipid.
30489236	2	4	theme	novel	101:105	arg1	actinobacterium					107:121	A novel actinobacterium	99:121	A novel actinobacterium	99:121	A novel actinobacterium, designated strain SYSU K10002T, was isolated from a soil sample collected from a karst cave in Xingyi county, Guizhou province, south-western China.
30489236	12	5	theme	K10002T	1410:1416	arg1	%					1430:1430	67.4 mol%	1422:1430	67.4 mol%	1422:1430	The genomic DNA G+C content of strain SYSU K10002T was 67.4 mol%.
30489236	12	5	theme	K10002T	1410:1416	arg1	G+C content					1383:1393	The genomic DNA G+C content	1367:1393	The genomic DNA G+C content of strain SYSU K10002T	1367:1416	The genomic DNA G+C content of strain SYSU K10002T was 67.4 mol%.
30489236	13	6	dep	data	1488:1491	arg1	basis					1440:1444	basis	1440:1444	basis	1440:1444	On the basis of phenotypic, genotypic and phylogenetic data, strain SYSU K10002T represents a novel species of the genus Nocardia, for which the name Nocardiaaurea sp.
30489236	13	6	dep	data	1488:1491	arg1	the					1436:1438	the	1436:1438	the	1436:1438	On the basis of phenotypic, genotypic and phylogenetic data, strain SYSU K10002T represents a novel species of the genus Nocardia, for which the name Nocardiaaurea sp.
30489236	13	7	theme	Nocardiaaurea	1583:1595	arg1	sp					1597:1598	the name Nocardiaaurea sp	1574:1598	the name Nocardiaaurea sp	1574:1598	On the basis of phenotypic, genotypic and phylogenetic data, strain SYSU K10002T represents a novel species of the genus Nocardia, for which the name Nocardiaaurea sp.
30489236	9	8	theme	 0	1163:1164	arg1	10-methyl					1166:1174	 0 10-methyl	1163:1174	C16 : 0, C18 : 1ω9c and C18 : 0 10-methyl	1134:1174	The major isoprenoid quinone was MK-8(H4,ω-cycl), while the major fatty acids (>10 %) were C16 : 0, C18 : 1ω9c and C18 : 0 10-methyl.
30489236	10	9	theme	phosphatidylinositol	1276:1295	arg1	mannoside					1297:1305	phosphatidylinositol mannoside	1276:1305	phosphatidylinositol mannoside	1276:1305	The polar lipids contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and an unidentified glycolipid.
30489236	9	10	theme	major	1103:1107	arg1	acids					1115:1119	the major fatty acids	1099:1119	the major fatty acids (>10 %)	1099:1127	The major isoprenoid quinone was MK-8(H4,ω-cycl), while the major fatty acids (>10 %) were C16 : 0, C18 : 1ω9c and C18 : 0 10-methyl.
30489236	9	10	theme	major	1103:1107	arg1	%					1126:1126	>10 %	1122:1126	>10 %	1122:1126	The major isoprenoid quinone was MK-8(H4,ω-cycl), while the major fatty acids (>10 %) were C16 : 0, C18 : 1ω9c and C18 : 0 10-methyl.
30489236	9	10	theme	major	1103:1107	arg1	C16 					1134:1137	C16 	1134:1137	C16 	1134:1137	The major isoprenoid quinone was MK-8(H4,ω-cycl), while the major fatty acids (>10 %) were C16 : 0, C18 : 1ω9c and C18 : 0 10-methyl.
30489236	13	11	theme	SYSU	1501:1504	arg1	K10002T					1506:1512	strain SYSU K10002T	1494:1512	strain SYSU K10002T	1494:1512	On the basis of phenotypic, genotypic and phylogenetic data, strain SYSU K10002T represents a novel species of the genus Nocardia, for which the name Nocardiaaurea sp.
30489236	7	12	theme	diagnostic	927:936	arg1	acid					946:949	the diagnostic diamino acid	923:949	the diagnostic diamino acid in the cell-wall peptidoglycan	923:980	In addition, meso-diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan.
30489236	7	12	theme	diagnostic	927:936	arg1	acid					914:917	meso-diaminopimelic acid	894:917	meso-diaminopimelic acid	894:917	In addition, meso-diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan.
30489236	11	13	theme	Mycolic	1339:1345	arg1	acids					1347:1351	Mycolic acids	1339:1351	Mycolic acids	1339:1351	Mycolic acids were present.
30489236	6	14	theme	Nocardia	851:858	arg1	strains					830:836	the closely related type strains	805:836	the closely related type strains of the genus Nocardia	805:858	DNA-DNA hybridization values between strain SYSU K10002T and the closely related type strains of the genus Nocardia were less than 70 %.
30489236	6	14	theme	Nocardia	851:858	arg1	strain					781:786	strain SYSU K10002T and the closely related type strains of the genus Nocardia	781:858	strain	781:786	DNA-DNA hybridization values between strain SYSU K10002T and the closely related type strains of the genus Nocardia were less than 70 %.
30489236	8	15	theme	whole-cell	987:996	arg1	arabinose					1010:1018	arabinose	1010:1018	arabinose	1010:1018	The whole-cell sugars were arabinose, ribose and galactose.
30489236	8	15	theme	whole-cell	987:996	arg1	sugars					998:1003	The whole-cell sugars	983:1003	The whole-cell sugars	983:1003	The whole-cell sugars were arabinose, ribose and galactose.
30489236	9	16	theme	fatty	1109:1113	arg1	acids					1115:1119	the major fatty acids	1099:1119	the major fatty acids (>10 %)	1099:1127	The major isoprenoid quinone was MK-8(H4,ω-cycl), while the major fatty acids (>10 %) were C16 : 0, C18 : 1ω9c and C18 : 0 10-methyl.
30489236	9	16	theme	fatty	1109:1113	arg1	%					1126:1126	>10 %	1122:1126	>10 %	1122:1126	The major isoprenoid quinone was MK-8(H4,ω-cycl), while the major fatty acids (>10 %) were C16 : 0, C18 : 1ω9c and C18 : 0 10-methyl.
30489236	9	16	theme	fatty	1109:1113	arg1	C16 					1134:1137	C16 	1134:1137	C16 	1134:1137	The major isoprenoid quinone was MK-8(H4,ω-cycl), while the major fatty acids (>10 %) were C16 : 0, C18 : 1ω9c and C18 : 0 10-methyl.
30489236	6	17	dep	strain	781:786	arg1	K10002T					793:799	K10002T	793:799	K10002T	793:799	DNA-DNA hybridization values between strain SYSU K10002T and the closely related type strains of the genus Nocardia were less than 70 %.
30489236	9	18	dep	MK-8	1076:1079	arg1	H4					1081:1082	H4	1081:1082	H4	1081:1082	The major isoprenoid quinone was MK-8(H4,ω-cycl), while the major fatty acids (>10 %) were C16 : 0, C18 : 1ω9c and C18 : 0 10-methyl.
30489236	9	18	dep	MK-8	1076:1079	arg1	ω-cycl					1084:1089	ω-cycl	1084:1089	ω-cycl	1084:1089	The major isoprenoid quinone was MK-8(H4,ω-cycl), while the major fatty acids (>10 %) were C16 : 0, C18 : 1ω9c and C18 : 0 10-methyl.
30489236	5	19	theme	analysis	483:490	arg1	related					530:536	related	530:536	related	530:536	On the basis of 16S rRNA gene sequence similarities and phylogenetic analysis, strain SYSU K10002T was most closely related to the type strains of Nocardiaaltamirensis NBRC 108246T (99.0 % sequence similarity) and Nocardiatenerifensis NBRC 101015T (98.8 %) and is therefore considered to represent a member of the genus Nocardia.
30489236	13	20	theme	phylogenetic	1475:1486	arg1	data					1488:1491	phenotypic, genotypic and phylogenetic data	1449:1491	phenotypic, genotypic and phylogenetic data	1449:1491	On the basis of phenotypic, genotypic and phylogenetic data, strain SYSU K10002T represents a novel species of the genus Nocardia, for which the name Nocardiaaurea sp.
30489236	2	21	theme	SYSU	142:145	arg1	K10002T					147:153	strain SYSU K10002T	135:153	strain SYSU K10002T	135:153	A novel actinobacterium, designated strain SYSU K10002T, was isolated from a soil sample collected from a karst cave in Xingyi county, Guizhou province, south-western China.
30489236	4	22	theme	strain	369:374	arg1	Cells					356:360	Cells	356:360	Cells of the strain	356:374	Cells of the strain were aerobic and Gram-stain-positive.
30489236	5	23	dep	related	530:536	arg1	%					668:668	98.8 %	663:668	98.8 %	663:668	On the basis of 16S rRNA gene sequence similarities and phylogenetic analysis, strain SYSU K10002T was most closely related to the type strains of Nocardiaaltamirensis NBRC 108246T (99.0 % sequence similarity) and Nocardiatenerifensis NBRC 101015T (98.8 %) and is therefore considered to represent a member of the genus Nocardia.
30489236	2	24	theme	strain	135:140	arg1	K10002T					147:153	strain SYSU K10002T	135:153	strain SYSU K10002T	135:153	A novel actinobacterium, designated strain SYSU K10002T, was isolated from a soil sample collected from a karst cave in Xingyi county, Guizhou province, south-western China.
30489236	5	25	theme	99.0 	596:600	arg1	%					601:601	%	601:601	%	601:601	On the basis of 16S rRNA gene sequence similarities and phylogenetic analysis, strain SYSU K10002T was most closely related to the type strains of Nocardiaaltamirensis NBRC 108246T (99.0 % sequence similarity) and Nocardiatenerifensis NBRC 101015T (98.8 %) and is therefore considered to represent a member of the genus Nocardia.
30489236	6	26	theme	DNA-DNA	744:750	arg1	values					766:771	DNA-DNA hybridization values	744:771	DNA-DNA hybridization values between strain SYSU K10002T and the closely related type strains of the genus Nocardia	744:858	DNA-DNA hybridization values between strain SYSU K10002T and the closely related type strains of the genus Nocardia were less than 70 %.
30489236	2	27	from	cave	211:214	arg1	county					226:231	county	226:231	county	226:231	A novel actinobacterium, designated strain SYSU K10002T, was isolated from a soil sample collected from a karst cave in Xingyi county, Guizhou province, south-western China.
30489236	2	27	from	cave	211:214	arg1	province					242:249	province	242:249	province	242:249	A novel actinobacterium, designated strain SYSU K10002T, was isolated from a soil sample collected from a karst cave in Xingyi county, Guizhou province, south-western China.
30489236	2	27	from	cave	211:214	arg1	China					266:270	China	266:270	China	266:270	A novel actinobacterium, designated strain SYSU K10002T, was isolated from a soil sample collected from a karst cave in Xingyi county, Guizhou province, south-western China.
30489236	0	28	theme	aurea	9:13	arg1	sp					15:16	Nocardia aurea sp	0:16	Nocardia aurea sp.	0:17	Nocardia aurea sp.
30489236	5	29	theme	%	601:601	arg1	similarity					612:621	99.0 % sequence similarity	596:621	99.0 % sequence similarity	596:621	On the basis of 16S rRNA gene sequence similarities and phylogenetic analysis, strain SYSU K10002T was most closely related to the type strains of Nocardiaaltamirensis NBRC 108246T (99.0 % sequence similarity) and Nocardiatenerifensis NBRC 101015T (98.8 %) and is therefore considered to represent a member of the genus Nocardia.
30489236	5	29	theme	%	601:601	arg1	108246T					587:593	108246T	587:593	108246T	587:593	On the basis of 16S rRNA gene sequence similarities and phylogenetic analysis, strain SYSU K10002T was most closely related to the type strains of Nocardiaaltamirensis NBRC 108246T (99.0 % sequence similarity) and Nocardiatenerifensis NBRC 101015T (98.8 %) and is therefore considered to represent a member of the genus Nocardia.
30489236	1	30	theme	karstic	65:71	arg1	environment					86:96	a karstic subterranean environment	63:96	a karstic subterranean environment	63:96	nov., a novel actinobacterium isolated from a karstic subterranean environment.
30489236	5	31	theme	101015T	654:660	arg1	strains					550:556	the type strains	541:556	the type strains of Nocardiaaltamirensis NBRC 108246T (99.0 % sequence similarity) and Nocardiatenerifensis NBRC 101015T	541:660	On the basis of 16S rRNA gene sequence similarities and phylogenetic analysis, strain SYSU K10002T was most closely related to the type strains of Nocardiaaltamirensis NBRC 108246T (99.0 % sequence similarity) and Nocardiatenerifensis NBRC 101015T (98.8 %) and is therefore considered to represent a member of the genus Nocardia.
30489236	0	32	theme	Nocardia	0:7	arg1	sp					15:16	Nocardia aurea sp	0:16	Nocardia aurea sp.	0:17	Nocardia aurea sp.
30489236	5	33	theme	sequence	603:610	arg1	similarity					612:621	99.0 % sequence similarity	596:621	99.0 % sequence similarity	596:621	On the basis of 16S rRNA gene sequence similarities and phylogenetic analysis, strain SYSU K10002T was most closely related to the type strains of Nocardiaaltamirensis NBRC 108246T (99.0 % sequence similarity) and Nocardiatenerifensis NBRC 101015T (98.8 %) and is therefore considered to represent a member of the genus Nocardia.
30489236	5	33	theme	sequence	603:610	arg1	108246T					587:593	108246T	587:593	108246T	587:593	On the basis of 16S rRNA gene sequence similarities and phylogenetic analysis, strain SYSU K10002T was most closely related to the type strains of Nocardiaaltamirensis NBRC 108246T (99.0 % sequence similarity) and Nocardiatenerifensis NBRC 101015T (98.8 %) and is therefore considered to represent a member of the genus Nocardia.
30489236	5	34	theme	type	545:548	arg1	strains					550:556	the type strains	541:556	the type strains of Nocardiaaltamirensis NBRC 108246T (99.0 % sequence similarity) and Nocardiatenerifensis NBRC 101015T	541:660	On the basis of 16S rRNA gene sequence similarities and phylogenetic analysis, strain SYSU K10002T was most closely related to the type strains of Nocardiaaltamirensis NBRC 108246T (99.0 % sequence similarity) and Nocardiatenerifensis NBRC 101015T (98.8 %) and is therefore considered to represent a member of the genus Nocardia.
30489236	1	35	theme	subterranean	73:84	arg1	environment					86:96	a karstic subterranean environment	63:96	a karstic subterranean environment	63:96	nov., a novel actinobacterium isolated from a karstic subterranean environment.
30489236	5	36	theme	16S	430:432	arg1	rRNA					434:437	16S rRNA gene sequence similarities and phylogenetic analysis	430:490	rRNA	434:437	On the basis of 16S rRNA gene sequence similarities and phylogenetic analysis, strain SYSU K10002T was most closely related to the type strains of Nocardiaaltamirensis NBRC 108246T (99.0 % sequence similarity) and Nocardiatenerifensis NBRC 101015T (98.8 %) and is therefore considered to represent a member of the genus Nocardia.
30489236	7	37	from	acid	946:949	arg1	addition					884:891	addition	884:891	addition	884:891	In addition, meso-diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan.
30489236	7	37	from	acid	946:949	arg1	peptidoglycan					968:980	the cell-wall peptidoglycan	954:980	the cell-wall peptidoglycan	954:980	In addition, meso-diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan.
30489236	3	38	theme	polyphasic	335:344	arg1	approach					346:353	a polyphasic approach	333:353	a polyphasic approach	333:353	The taxonomic position of the strain was investigated using a polyphasic approach.
30489236	10	39	contain	contained	1194:1202	arg2	mannoside					1297:1305	phosphatidylinositol mannoside	1276:1305	phosphatidylinositol mannoside	1276:1305	The polar lipids contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and an unidentified glycolipid.
30489236	10	39	contain	contained	1194:1202	arg2	diphosphatidylglycerol					1204:1225	diphosphatidylglycerol	1204:1225	diphosphatidylglycerol	1204:1225	The polar lipids contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and an unidentified glycolipid.
30489236	10	39	contain	contained	1194:1202	arg1	lipids					1187:1192	The polar lipids	1177:1192	The polar lipids	1177:1192	The polar lipids contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and an unidentified glycolipid.
30489236	10	39	contain	contained	1194:1202	arg2	phosphatidylethanolamine					1228:1251	phosphatidylethanolamine	1228:1251	phosphatidylethanolamine	1228:1251	The polar lipids contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and an unidentified glycolipid.
30489236	10	39	contain	contained	1194:1202	arg2	glycolipid					1327:1336	an unidentified glycolipid	1311:1336	an unidentified glycolipid	1311:1336	The polar lipids contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and an unidentified glycolipid.
30489236	10	39	contain	contained	1194:1202	arg2	phosphatidylinositol					1254:1273	phosphatidylinositol	1254:1273	phosphatidylinositol	1254:1273	The polar lipids contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and an unidentified glycolipid.
30489236	13	40	theme	genus	1548:1552	arg1	Nocardia					1554:1561	the genus Nocardia	1544:1561	the genus Nocardia	1544:1561	On the basis of phenotypic, genotypic and phylogenetic data, strain SYSU K10002T represents a novel species of the genus Nocardia, for which the name Nocardiaaurea sp.
30489236	5	41	theme	108246T	587:593	arg1	strains					550:556	the type strains	541:556	the type strains of Nocardiaaltamirensis NBRC 108246T (99.0 % sequence similarity) and Nocardiatenerifensis NBRC 101015T	541:660	On the basis of 16S rRNA gene sequence similarities and phylogenetic analysis, strain SYSU K10002T was most closely related to the type strains of Nocardiaaltamirensis NBRC 108246T (99.0 % sequence similarity) and Nocardiatenerifensis NBRC 101015T (98.8 %) and is therefore considered to represent a member of the genus Nocardia.
30489236	5	42	theme	rRNA	434:437	arg1	related					530:536	related	530:536	related	530:536	On the basis of 16S rRNA gene sequence similarities and phylogenetic analysis, strain SYSU K10002T was most closely related to the type strains of Nocardiaaltamirensis NBRC 108246T (99.0 % sequence similarity) and Nocardiatenerifensis NBRC 101015T (98.8 %) and is therefore considered to represent a member of the genus Nocardia.
30489236	2	43	attach	isolated	160:167	arg1	sample					181:186	a soil sample	174:186	a soil sample collected from a karst cave in Xingyi county, Guizhou province, south-western China	174:270	A novel actinobacterium, designated strain SYSU K10002T, was isolated from a soil sample collected from a karst cave in Xingyi county, Guizhou province, south-western China.
30489236	2	43	attach	isolated	160:167	arg2	actinobacterium					107:121	A novel actinobacterium	99:121	A novel actinobacterium	99:121	A novel actinobacterium, designated strain SYSU K10002T, was isolated from a soil sample collected from a karst cave in Xingyi county, Guizhou province, south-western China.
30489236	13	44	theme	Nocardia	1554:1561	arg1	species					1533:1539	a novel species	1525:1539	a novel species	1525:1539	On the basis of phenotypic, genotypic and phylogenetic data, strain SYSU K10002T represents a novel species of the genus Nocardia, for which the name Nocardiaaurea sp.
30489236	12	45	theme	67.4 mol	1422:1429	arg1	%					1430:1430	67.4 mol%	1422:1430	67.4 mol%	1422:1430	The genomic DNA G+C content of strain SYSU K10002T was 67.4 mol%.
30489236	12	45	theme	67.4 mol	1422:1429	arg1	G+C content					1383:1393	The genomic DNA G+C content	1367:1393	The genomic DNA G+C content of strain SYSU K10002T	1367:1416	The genomic DNA G+C content of strain SYSU K10002T was 67.4 mol%.
30489236	13	46	theme	strain	1494:1499	arg1	K10002T					1506:1512	strain SYSU K10002T	1494:1512	strain SYSU K10002T	1494:1512	On the basis of phenotypic, genotypic and phylogenetic data, strain SYSU K10002T represents a novel species of the genus Nocardia, for which the name Nocardiaaurea sp.
30489236	5	47	theme	gene	439:442	arg1	similarities					453:464	gene sequence similarities	439:464	gene sequence similarities	439:464	On the basis of 16S rRNA gene sequence similarities and phylogenetic analysis, strain SYSU K10002T was most closely related to the type strains of Nocardiaaltamirensis NBRC 108246T (99.0 % sequence similarity) and Nocardiatenerifensis NBRC 101015T (98.8 %) and is therefore considered to represent a member of the genus Nocardia.
30489236	9	48	theme	major	1047:1051	arg1	MK-8					1076:1079	MK-8	1076:1079	MK-8	1076:1079	The major isoprenoid quinone was MK-8(H4,ω-cycl), while the major fatty acids (>10 %) were C16 : 0, C18 : 1ω9c and C18 : 0 10-methyl.
30489236	9	48	theme	major	1047:1051	arg1	quinone					1064:1070	The major isoprenoid quinone	1043:1070	The major isoprenoid quinone	1043:1070	The major isoprenoid quinone was MK-8(H4,ω-cycl), while the major fatty acids (>10 %) were C16 : 0, C18 : 1ω9c and C18 : 0 10-methyl.
30489236	5	49	theme	genus	728:732	arg1	Nocardia					734:741	the genus Nocardia	724:741	the genus Nocardia	724:741	On the basis of 16S rRNA gene sequence similarities and phylogenetic analysis, strain SYSU K10002T was most closely related to the type strains of Nocardiaaltamirensis NBRC 108246T (99.0 % sequence similarity) and Nocardiatenerifensis NBRC 101015T (98.8 %) and is therefore considered to represent a member of the genus Nocardia.
30489236	15	50	theme	39849T=DSM	1658:1667	arg1	103986T					1669:1675	=KCTC 39849T=DSM 103986T	1652:1675	=KCTC 39849T=DSM 103986T	1652:1675	The type strain is SYSU K10002T (=KCTC 39849T=DSM 103986T).
30489236	15	50	theme	39849T=DSM	1658:1667	arg1	K10002T					1643:1649	SYSU K10002T	1638:1649	SYSU K10002T (=KCTC 39849T=DSM 103986T)	1638:1676	The type strain is SYSU K10002T (=KCTC 39849T=DSM 103986T).
30489236	6	51	theme	type	825:828	arg1	strains					830:836	the closely related type strains	805:836	the closely related type strains of the genus Nocardia	805:858	DNA-DNA hybridization values between strain SYSU K10002T and the closely related type strains of the genus Nocardia were less than 70 %.
30489236	13	52	theme	phenotypic	1449:1458	arg1	data					1488:1491	phenotypic, genotypic and phylogenetic data	1449:1491	phenotypic, genotypic and phylogenetic data	1449:1491	On the basis of phenotypic, genotypic and phylogenetic data, strain SYSU K10002T represents a novel species of the genus Nocardia, for which the name Nocardiaaurea sp.
30489236	5	53	theme	sequence	444:451	arg1	similarities					453:464	gene sequence similarities	439:464	gene sequence similarities	439:464	On the basis of 16S rRNA gene sequence similarities and phylogenetic analysis, strain SYSU K10002T was most closely related to the type strains of Nocardiaaltamirensis NBRC 108246T (99.0 % sequence similarity) and Nocardiatenerifensis NBRC 101015T (98.8 %) and is therefore considered to represent a member of the genus Nocardia.
30489236	5	54	theme	Nocardia	734:741	arg1	member					714:719	a member	712:719	a member of the genus Nocardia	712:741	On the basis of 16S rRNA gene sequence similarities and phylogenetic analysis, strain SYSU K10002T was most closely related to the type strains of Nocardiaaltamirensis NBRC 108246T (99.0 % sequence similarity) and Nocardiatenerifensis NBRC 101015T (98.8 %) and is therefore considered to represent a member of the genus Nocardia.
30489236	6	55	theme	related	817:823	arg1	strains					830:836	the closely related type strains	805:836	the closely related type strains of the genus Nocardia	805:858	DNA-DNA hybridization values between strain SYSU K10002T and the closely related type strains of the genus Nocardia were less than 70 %.
30489236	2	56	theme	karst	205:209	arg1	cave					211:214	a karst cave	203:214	a karst cave in Xingyi county, Guizhou province, south-western China	203:270	A novel actinobacterium, designated strain SYSU K10002T, was isolated from a soil sample collected from a karst cave in Xingyi county, Guizhou province, south-western China.
30489236	7	57	theme	cell-wall	958:966	arg1	peptidoglycan					968:980	the cell-wall peptidoglycan	954:980	the cell-wall peptidoglycan	954:980	In addition, meso-diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan.
30489236	13	58	theme	novel	1527:1531	arg1	species					1533:1539	a novel species	1525:1539	a novel species	1525:1539	On the basis of phenotypic, genotypic and phylogenetic data, strain SYSU K10002T represents a novel species of the genus Nocardia, for which the name Nocardiaaurea sp.
30489236	13	59	theme	genotypic	1461:1469	arg1	data					1488:1491	phenotypic, genotypic and phylogenetic data	1449:1491	phenotypic, genotypic and phylogenetic data	1449:1491	On the basis of phenotypic, genotypic and phylogenetic data, strain SYSU K10002T represents a novel species of the genus Nocardia, for which the name Nocardiaaurea sp.
30489236	12	60	theme	DNA	1379:1381	arg1	%					1430:1430	67.4 mol%	1422:1430	67.4 mol%	1422:1430	The genomic DNA G+C content of strain SYSU K10002T was 67.4 mol%.
30489236	12	60	theme	DNA	1379:1381	arg1	G+C content					1383:1393	The genomic DNA G+C content	1367:1393	The genomic DNA G+C content of strain SYSU K10002T	1367:1416	The genomic DNA G+C content of strain SYSU K10002T was 67.4 mol%.
30489236	3	61	theme	taxonomic	277:285	arg1	position					287:294	The taxonomic position	273:294	The taxonomic position of the strain	273:308	The taxonomic position of the strain was investigated using a polyphasic approach.
30489236	12	62	theme	strain	1398:1403	arg1	K10002T					1410:1416	strain SYSU K10002T	1398:1416	strain SYSU K10002T	1398:1416	The genomic DNA G+C content of strain SYSU K10002T was 67.4 mol%.
30489236	6	63	theme	genus	845:849	arg1	Nocardia					851:858	the genus Nocardia	841:858	the genus Nocardia	841:858	DNA-DNA hybridization values between strain SYSU K10002T and the closely related type strains of the genus Nocardia were less than 70 %.
30489236	15	64	theme	SYSU	1638:1641	arg1	103986T					1669:1675	=KCTC 39849T=DSM 103986T	1652:1675	=KCTC 39849T=DSM 103986T	1652:1675	The type strain is SYSU K10002T (=KCTC 39849T=DSM 103986T).
30489236	15	64	theme	SYSU	1638:1641	arg1	strain					1628:1633	The type strain	1619:1633	The type strain	1619:1633	The type strain is SYSU K10002T (=KCTC 39849T=DSM 103986T).
30489236	15	64	theme	SYSU	1638:1641	arg1	K10002T					1643:1649	SYSU K10002T	1638:1649	SYSU K10002T (=KCTC 39849T=DSM 103986T)	1638:1676	The type strain is SYSU K10002T (=KCTC 39849T=DSM 103986T).
30489236	10	65	theme	unidentified	1314:1325	arg1	glycolipid					1327:1336	an unidentified glycolipid	1311:1336	an unidentified glycolipid	1311:1336	The polar lipids contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and an unidentified glycolipid.
30489236	7	66	theme	meso-diaminopimelic	894:912	arg1	acid					946:949	the diagnostic diamino acid	923:949	the diagnostic diamino acid in the cell-wall peptidoglycan	923:980	In addition, meso-diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan.
30489236	7	66	theme	meso-diaminopimelic	894:912	arg1	acid					914:917	meso-diaminopimelic acid	894:917	meso-diaminopimelic acid	894:917	In addition, meso-diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan.
30489236	15	67	theme	=KCTC	1652:1656	arg1	103986T					1669:1675	=KCTC 39849T=DSM 103986T	1652:1675	=KCTC 39849T=DSM 103986T	1652:1675	The type strain is SYSU K10002T (=KCTC 39849T=DSM 103986T).
30489236	15	67	theme	=KCTC	1652:1656	arg1	K10002T					1643:1649	SYSU K10002T	1638:1649	SYSU K10002T (=KCTC 39849T=DSM 103986T)	1638:1676	The type strain is SYSU K10002T (=KCTC 39849T=DSM 103986T).
30489236	2	68	theme	soil	176:179	arg1	sample					181:186	a soil sample	174:186	a soil sample collected from a karst cave in Xingyi county, Guizhou province, south-western China	174:270	A novel actinobacterium, designated strain SYSU K10002T, was isolated from a soil sample collected from a karst cave in Xingyi county, Guizhou province, south-western China.
30489236	7	69	theme	diamino	938:944	arg1	acid					946:949	the diagnostic diamino acid	923:949	the diagnostic diamino acid in the cell-wall peptidoglycan	923:980	In addition, meso-diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan.
30489236	7	69	theme	diamino	938:944	arg1	acid					914:917	meso-diaminopimelic acid	894:917	meso-diaminopimelic acid	894:917	In addition, meso-diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan.
30489236	9	70	theme	isoprenoid	1053:1062	arg1	MK-8					1076:1079	MK-8	1076:1079	MK-8	1076:1079	The major isoprenoid quinone was MK-8(H4,ω-cycl), while the major fatty acids (>10 %) were C16 : 0, C18 : 1ω9c and C18 : 0 10-methyl.
30489236	9	70	theme	isoprenoid	1053:1062	arg1	quinone					1064:1070	The major isoprenoid quinone	1043:1070	The major isoprenoid quinone	1043:1070	The major isoprenoid quinone was MK-8(H4,ω-cycl), while the major fatty acids (>10 %) were C16 : 0, C18 : 1ω9c and C18 : 0 10-methyl.
30489236	6	71	theme	hybridization	752:764	arg1	values					766:771	DNA-DNA hybridization values	744:771	DNA-DNA hybridization values between strain SYSU K10002T and the closely related type strains of the genus Nocardia	744:858	DNA-DNA hybridization values between strain SYSU K10002T and the closely related type strains of the genus Nocardia were less than 70 %.
30489236	5	72	theme	phylogenetic	470:481	arg1	analysis					483:490	phylogenetic analysis	470:490	phylogenetic analysis	470:490	On the basis of 16S rRNA gene sequence similarities and phylogenetic analysis, strain SYSU K10002T was most closely related to the type strains of Nocardiaaltamirensis NBRC 108246T (99.0 % sequence similarity) and Nocardiatenerifensis NBRC 101015T (98.8 %) and is therefore considered to represent a member of the genus Nocardia.
30489236	12	73	theme	SYSU	1405:1408	arg1	K10002T					1410:1416	strain SYSU K10002T	1398:1416	strain SYSU K10002T	1398:1416	The genomic DNA G+C content of strain SYSU K10002T was 67.4 mol%.
30489236	5	74	theme	strain	493:498	arg1	K10002T					505:511	strain SYSU K10002T	493:511	strain SYSU K10002T	493:511	On the basis of 16S rRNA gene sequence similarities and phylogenetic analysis, strain SYSU K10002T was most closely related to the type strains of Nocardiaaltamirensis NBRC 108246T (99.0 % sequence similarity) and Nocardiatenerifensis NBRC 101015T (98.8 %) and is therefore considered to represent a member of the genus Nocardia.
30489236	3	75	theme	strain	303:308	arg1	position					287:294	The taxonomic position	273:294	The taxonomic position of the strain	273:308	The taxonomic position of the strain was investigated using a polyphasic approach.
30489236	12	76	theme	genomic	1371:1377	arg1	%					1430:1430	67.4 mol%	1422:1430	67.4 mol%	1422:1430	The genomic DNA G+C content of strain SYSU K10002T was 67.4 mol%.
30489236	12	76	theme	genomic	1371:1377	arg1	G+C content					1383:1393	The genomic DNA G+C content	1367:1393	The genomic DNA G+C content of strain SYSU K10002T	1367:1416	The genomic DNA G+C content of strain SYSU K10002T was 67.4 mol%.
30489236	1	77	theme	novel	27:31	arg1	actinobacterium					33:47	a novel actinobacterium	25:47	a novel actinobacterium	25:47	nov., a novel actinobacterium isolated from a karstic subterranean environment.
30489236	1	77	theme	novel	27:31	arg1	nov.					19:22	nov.	19:22	nov.	19:22	nov., a novel actinobacterium isolated from a karstic subterranean environment.
30489236	5	78	theme	SYSU	500:503	arg1	K10002T					505:511	strain SYSU K10002T	493:511	strain SYSU K10002T	493:511	On the basis of 16S rRNA gene sequence similarities and phylogenetic analysis, strain SYSU K10002T was most closely related to the type strains of Nocardiaaltamirensis NBRC 108246T (99.0 % sequence similarity) and Nocardiatenerifensis NBRC 101015T (98.8 %) and is therefore considered to represent a member of the genus Nocardia.
30489236	9	79	dep	C16 	1134:1137	arg1	C18 					1143:1146	C18 	1143:1146	C18 	1143:1146	The major isoprenoid quinone was MK-8(H4,ω-cycl), while the major fatty acids (>10 %) were C16 : 0, C18 : 1ω9c and C18 : 0 10-methyl.
30489236	9	79	dep	C16 	1134:1137	arg1	C18 					1158:1161	C18 	1158:1161	C18 	1158:1161	The major isoprenoid quinone was MK-8(H4,ω-cycl), while the major fatty acids (>10 %) were C16 : 0, C18 : 1ω9c and C18 : 0 10-methyl.
30489236	9	79	dep	C16 	1134:1137	arg1	10-methyl					1166:1174	 0 10-methyl	1163:1174	C16 : 0, C18 : 1ω9c and C18 : 0 10-methyl	1134:1174	The major isoprenoid quinone was MK-8(H4,ω-cycl), while the major fatty acids (>10 %) were C16 : 0, C18 : 1ω9c and C18 : 0 10-methyl.
30489236	9	79	dep	C16 	1134:1137	arg1	 1ω9c					1148:1152	 1ω9c	1148:1152	 1ω9c	1148:1152	The major isoprenoid quinone was MK-8(H4,ω-cycl), while the major fatty acids (>10 %) were C16 : 0, C18 : 1ω9c and C18 : 0 10-methyl.
30207517	8	0	theme	genomic	1184:1190	arg1	G+C content					1196:1206	The genomic DNA G+C content	1180:1206	The genomic DNA G+C content of the strain AK72T	1180:1226	The genomic DNA G+C content of the strain AK72T was 36.8 mol%.
30207517	8	0	theme	genomic	1184:1190	arg1	%					1240:1240	36.8 mol%	1232:1240	36.8 mol%	1232:1240	The genomic DNA G+C content of the strain AK72T was 36.8 mol%.
30207517	3	1	theme	entire	418:423	arg1	margin					425:430	an entire margin	415:430	an entire margin after 48 h incubation at 37 °C with pH 9	415:471	The colony appears circular, shiny, smooth, translucent or slightly pale in colour and convex with an entire margin after 48 h incubation at 37 °C with pH 9.
30207517	6	2	contain	contained	900:908	arg1	 0					849:850	 0	849:850	 0	849:850	The predominant fatty acids were iso-C15 : 0, anteiso-C15 : 0 and iso-C16 : 0 and the cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid.
30207517	6	2	contain	contained	900:908	arg2	acid					961:964	the diagnostic diamino acid	938:964	the diagnostic diamino acid	938:964	The predominant fatty acids were iso-C15 : 0, anteiso-C15 : 0 and iso-C16 : 0 and the cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid.
30207517	6	2	contain	contained	900:908	arg2	acid					930:933	meso-diaminopimelic acid	910:933	meso-diaminopimelic acid	910:933	The predominant fatty acids were iso-C15 : 0, anteiso-C15 : 0 and iso-C16 : 0 and the cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid.
30207517	6	2	contain	contained	900:908	arg1	peptidoglycan					886:898	the cell-wall peptidoglycan	872:898	the cell-wall peptidoglycan	872:898	The predominant fatty acids were iso-C15 : 0, anteiso-C15 : 0 and iso-C16 : 0 and the cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid.
30207517	6	2	contain	contained	900:908	arg1	 0					832:833	 0	832:833	 0	832:833	The predominant fatty acids were iso-C15 : 0, anteiso-C15 : 0 and iso-C16 : 0 and the cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid.
30207517	6	2	contain	contained	900:908	arg1	anteiso-C15 					836:847	anteiso-C15 	836:847	anteiso-C15 	836:847	The predominant fatty acids were iso-C15 : 0, anteiso-C15 : 0 and iso-C16 : 0 and the cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid.
30207517	6	2	contain	contained	900:908	arg1	 0					865:866	 0	865:866	 0	865:866	The predominant fatty acids were iso-C15 : 0, anteiso-C15 : 0 and iso-C16 : 0 and the cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid.
30207517	4	3	theme	w/v	567:569	arg1	 NaCl					591:595	optimum 3-5 % NaCl	578:595	optimum 3-5 % NaCl	578:595	Growth of the bacterium occurred at 10-42 °C (optimum, 25-37 °C), at salinities of 0.5-10 % (w/v) NaCl (optimum 3-5 % NaCl) and pH of 6-10 (optimum pH 9).
30207517	4	3	theme	w/v	567:569	arg1	NaCl					572:575	0.5-10 % (w/v) NaCl	557:575	0.5-10 % (w/v) NaCl (optimum 3-5 % NaCl)	557:596	Growth of the bacterium occurred at 10-42 °C (optimum, 25-37 °C), at salinities of 0.5-10 % (w/v) NaCl (optimum 3-5 % NaCl) and pH of 6-10 (optimum pH 9).
30207517	3	4	from	37 °C	457:461	arg1	incubation					443:452	48 h incubation	438:452	48 h incubation at 37 °C with pH 9	438:471	The colony appears circular, shiny, smooth, translucent or slightly pale in colour and convex with an entire margin after 48 h incubation at 37 °C with pH 9.
30207517	9	5	theme	16S	1272:1274	arg1	sequence					1286:1293	the 16S rRNA gene sequence	1268:1293	the 16S rRNA gene sequence	1268:1293	Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain AK72T was closely related to Bacillus cellulosilyticus (96.5 %) and Bacillus vedderi (96.3 %), but the novel strain AK72T formed a separate clade with Bacillus aurantiacus whereas B. cellulosilyticus and B. vedderi were clustered in a separate clade.
30207517	8	6	theme	36.8 mol	1232:1239	arg1	G+C content					1196:1206	The genomic DNA G+C content	1180:1206	The genomic DNA G+C content of the strain AK72T	1180:1226	The genomic DNA G+C content of the strain AK72T was 36.8 mol%.
30207517	8	6	theme	36.8 mol	1232:1239	arg1	%					1240:1240	36.8 mol%	1232:1240	36.8 mol%	1232:1240	The genomic DNA G+C content of the strain AK72T was 36.8 mol%.
30207517	4	7	theme	optimum	614:620	arg1	pH					622:623	optimum pH 9	614:625	optimum pH 9	614:625	Growth of the bacterium occurred at 10-42 °C (optimum, 25-37 °C), at salinities of 0.5-10 % (w/v) NaCl (optimum 3-5 % NaCl) and pH of 6-10 (optimum pH 9).
30207517	4	7	theme	optimum	614:620	arg1	pH					602:603	pH	602:603	pH of 6-10 (optimum pH 9)	602:626	Growth of the bacterium occurred at 10-42 °C (optimum, 25-37 °C), at salinities of 0.5-10 % (w/v) NaCl (optimum 3-5 % NaCl) and pH of 6-10 (optimum pH 9).
30207517	9	8	theme	strain	1426:1431	arg1	AK72T					1433:1437	the novel strain AK72T	1416:1437	the novel strain AK72T formed a separate clade with Bacillus aurantiacus whereas B. cellulosilyticus and B. vedderi	1416:1530	Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain AK72T was closely related to Bacillus cellulosilyticus (96.5 %) and Bacillus vedderi (96.3 %), but the novel strain AK72T formed a separate clade with Bacillus aurantiacus whereas B. cellulosilyticus and B. vedderi were clustered in a separate clade.
30207517	3	9	with	circular	335:342	arg1	margin					425:430	an entire margin	415:430	an entire margin after 48 h incubation at 37 °C with pH 9	415:471	The colony appears circular, shiny, smooth, translucent or slightly pale in colour and convex with an entire margin after 48 h incubation at 37 °C with pH 9.
30207517	6	10	theme	diamino	953:959	arg1	acid					961:964	the diagnostic diamino acid	938:964	the diagnostic diamino acid	938:964	The predominant fatty acids were iso-C15 : 0, anteiso-C15 : 0 and iso-C16 : 0 and the cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid.
30207517	6	10	theme	diamino	953:959	arg1	acid					930:933	meso-diaminopimelic acid	910:933	meso-diaminopimelic acid	910:933	The predominant fatty acids were iso-C15 : 0, anteiso-C15 : 0 and iso-C16 : 0 and the cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid.
30207517	9	11	theme	rRNA	1276:1279	arg1	sequence					1286:1293	the 16S rRNA gene sequence	1268:1293	the 16S rRNA gene sequence	1268:1293	Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain AK72T was closely related to Bacillus cellulosilyticus (96.5 %) and Bacillus vedderi (96.3 %), but the novel strain AK72T formed a separate clade with Bacillus aurantiacus whereas B. cellulosilyticus and B. vedderi were clustered in a separate clade.
30207517	10	12	theme	phylogenetic	1638:1649	arg1	data					1651:1654	phylogenetic data	1638:1654	phylogenetic data	1638:1654	The above data in combination with the phenotypic characteristics and phylogenetic data inferred that strain AK72T represents a novel species of the genus Bacillus, for which the name Bacillusshivajii sp.
30207517	4	13	theme	pH	602:603	arg1	salinities					543:552	salinities	543:552	salinities of 0.5-10 % (w/v) NaCl (optimum 3-5 % NaCl) and pH of 6-10 (optimum pH 9)	543:626	Growth of the bacterium occurred at 10-42 °C (optimum, 25-37 °C), at salinities of 0.5-10 % (w/v) NaCl (optimum 3-5 % NaCl) and pH of 6-10 (optimum pH 9).
30207517	1	14	theme	water	44:48	arg1	sample					50:55	a water sample	42:55	a water sample of Sambhar salt lake, India	42:83	nov., isolated from a water sample of Sambhar salt lake, India.
30207517	10	15	from	data	1651:1654	arg1	combination					1586:1596	combination	1586:1596	combination with the phenotypic characteristics	1586:1632	The above data in combination with the phenotypic characteristics and phylogenetic data inferred that strain AK72T represents a novel species of the genus Bacillus, for which the name Bacillusshivajii sp.
30207517	4	16	theme	bacterium	488:496	arg1	Growth					474:479	Growth	474:479	Growth of the bacterium	474:496	Growth of the bacterium occurred at 10-42 °C (optimum, 25-37 °C), at salinities of 0.5-10 % (w/v) NaCl (optimum 3-5 % NaCl) and pH of 6-10 (optimum pH 9).
30207517	12	17	theme	12636T=KCTC	1823:1833	arg1	32183T					1846:1851	=MTCC 12636T=KCTC 33981T=JCM 32183T	1817:1851	=MTCC 12636T=KCTC 33981T=JCM 32183T	1817:1851	The type strain is AK72T (=MTCC 12636T=KCTC 33981T=JCM 32183T).
30207517	12	17	theme	12636T=KCTC	1823:1833	arg1	AK72T					1810:1814	AK72T	1810:1814	AK72T (=MTCC 12636T=KCTC 33981T=JCM 32183T)	1810:1852	The type strain is AK72T (=MTCC 12636T=KCTC 33981T=JCM 32183T).
30207517	2	18	theme	rod-shaped	115:124	arg1	bacterium					195:203	rod-shaped, motile, spore-forming, strictly aerobic, alkali- and halo- tolerant bacterium	115:203	rod-shaped, motile, spore-forming, strictly aerobic, alkali- and halo- tolerant bacterium	115:203	A novel Gram-stain-positive, rod-shaped, motile, spore-forming, strictly aerobic, alkali- and halo- tolerant bacterium, designated strain AK72T, was isolated from a water sample collected from Sambhar salt lake, Rajasthan, India.
30207517	2	18	theme	rod-shaped	115:124	arg1	Gram-stain-positive					94:112	A novel Gram-stain-positive	86:112	A novel Gram-stain-positive	86:112	A novel Gram-stain-positive, rod-shaped, motile, spore-forming, strictly aerobic, alkali- and halo- tolerant bacterium, designated strain AK72T, was isolated from a water sample collected from Sambhar salt lake, Rajasthan, India.
30207517	2	19	dep	India	309:313	arg1	Rajasthan					298:306	Rajasthan	298:306	Rajasthan	298:306	A novel Gram-stain-positive, rod-shaped, motile, spore-forming, strictly aerobic, alkali- and halo- tolerant bacterium, designated strain AK72T, was isolated from a water sample collected from Sambhar salt lake, Rajasthan, India.
30207517	8	20	theme	DNA	1192:1194	arg1	G+C content					1196:1206	The genomic DNA G+C content	1180:1206	The genomic DNA G+C content of the strain AK72T	1180:1226	The genomic DNA G+C content of the strain AK72T was 36.8 mol%.
30207517	8	20	theme	DNA	1192:1194	arg1	%					1240:1240	36.8 mol%	1232:1240	36.8 mol%	1232:1240	The genomic DNA G+C content of the strain AK72T was 36.8 mol%.
30207517	6	21	theme	fatty	806:810	arg1	iso-C15 					823:830	iso-C15 	823:830	iso-C15 	823:830	The predominant fatty acids were iso-C15 : 0, anteiso-C15 : 0 and iso-C16 : 0 and the cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid.
30207517	6	21	theme	fatty	806:810	arg1	acids					812:816	The predominant fatty acids	790:816	The predominant fatty acids	790:816	The predominant fatty acids were iso-C15 : 0, anteiso-C15 : 0 and iso-C16 : 0 and the cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid.
30207517	10	22	theme	Bacillusshivajii	1752:1767	arg1	sp					1769:1770	the name Bacillusshivajii sp	1743:1770	the name Bacillusshivajii sp	1743:1770	The above data in combination with the phenotypic characteristics and phylogenetic data inferred that strain AK72T represents a novel species of the genus Bacillus, for which the name Bacillusshivajii sp.
30207517	4	23	theme	6-10	608:611	arg1	pH					622:623	optimum pH 9	614:625	optimum pH 9	614:625	Growth of the bacterium occurred at 10-42 °C (optimum, 25-37 °C), at salinities of 0.5-10 % (w/v) NaCl (optimum 3-5 % NaCl) and pH of 6-10 (optimum pH 9).
30207517	4	23	theme	6-10	608:611	arg1	pH					602:603	pH	602:603	pH of 6-10 (optimum pH 9)	602:626	Growth of the bacterium occurred at 10-42 °C (optimum, 25-37 °C), at salinities of 0.5-10 % (w/v) NaCl (optimum 3-5 % NaCl) and pH of 6-10 (optimum pH 9).
30207517	4	23	theme	6-10	608:611	arg1	NaCl					572:575	0.5-10 % (w/v) NaCl	557:575	0.5-10 % (w/v) NaCl (optimum 3-5 % NaCl)	557:596	Growth of the bacterium occurred at 10-42 °C (optimum, 25-37 °C), at salinities of 0.5-10 % (w/v) NaCl (optimum 3-5 % NaCl) and pH of 6-10 (optimum pH 9).
30207517	4	23	theme	6-10	608:611	arg1	 NaCl					591:595	optimum 3-5 % NaCl	578:595	optimum 3-5 % NaCl	578:595	Growth of the bacterium occurred at 10-42 °C (optimum, 25-37 °C), at salinities of 0.5-10 % (w/v) NaCl (optimum 3-5 % NaCl) and pH of 6-10 (optimum pH 9).
30207517	6	24	theme	cell-wall	876:884	arg1	peptidoglycan					886:898	the cell-wall peptidoglycan	872:898	the cell-wall peptidoglycan	872:898	The predominant fatty acids were iso-C15 : 0, anteiso-C15 : 0 and iso-C16 : 0 and the cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid.
30207517	0	25	theme	shivajii	9:16	arg1	sp					18:19	Bacillus shivajii sp	0:19	Bacillus shivajii sp.	0:20	Bacillus shivajii sp.
30207517	10	26	theme	name	1747:1750	arg1	sp					1769:1770	the name Bacillusshivajii sp	1743:1770	the name Bacillusshivajii sp	1743:1770	The above data in combination with the phenotypic characteristics and phylogenetic data inferred that strain AK72T represents a novel species of the genus Bacillus, for which the name Bacillusshivajii sp.
30207517	3	27	from	convex	403:408	arg1	pale					384:387	pale	384:387	pale	384:387	The colony appears circular, shiny, smooth, translucent or slightly pale in colour and convex with an entire margin after 48 h incubation at 37 °C with pH 9.
30207517	10	28	theme	phenotypic	1607:1616	arg1	characteristics					1618:1632	the phenotypic characteristics	1603:1632	the phenotypic characteristics	1603:1632	The above data in combination with the phenotypic characteristics and phylogenetic data inferred that strain AK72T represents a novel species of the genus Bacillus, for which the name Bacillusshivajii sp.
30207517	5	29	theme	nitrate	678:684	arg1	reductase					686:694	nitrate reductase	678:694	nitrate reductase	678:694	Strain AK72T was positive for oxidase, catalase, nitrate reductase, phenylalanine deaminase, ornithine decarboxylase, aesculinase, lipase and urease activities.
30207517	0	30	theme	Bacillus	0:7	arg1	sp					18:19	Bacillus shivajii sp	0:19	Bacillus shivajii sp.	0:20	Bacillus shivajii sp.
30207517	6	31	theme	predominant	794:804	arg1	iso-C15 					823:830	iso-C15 	823:830	iso-C15 	823:830	The predominant fatty acids were iso-C15 : 0, anteiso-C15 : 0 and iso-C16 : 0 and the cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid.
30207517	6	31	theme	predominant	794:804	arg1	acids					812:816	The predominant fatty acids	790:816	The predominant fatty acids	790:816	The predominant fatty acids were iso-C15 : 0, anteiso-C15 : 0 and iso-C16 : 0 and the cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid.
30207517	4	32	theme	optimum	578:584	arg1	 NaCl					591:595	optimum 3-5 % NaCl	578:595	optimum 3-5 % NaCl	578:595	Growth of the bacterium occurred at 10-42 °C (optimum, 25-37 °C), at salinities of 0.5-10 % (w/v) NaCl (optimum 3-5 % NaCl) and pH of 6-10 (optimum pH 9).
30207517	4	32	theme	optimum	578:584	arg1	NaCl					572:575	0.5-10 % (w/v) NaCl	557:575	0.5-10 % (w/v) NaCl (optimum 3-5 % NaCl)	557:596	Growth of the bacterium occurred at 10-42 °C (optimum, 25-37 °C), at salinities of 0.5-10 % (w/v) NaCl (optimum 3-5 % NaCl) and pH of 6-10 (optimum pH 9).
30207517	4	33	theme	%	590:590	arg1	 NaCl					591:595	optimum 3-5 % NaCl	578:595	optimum 3-5 % NaCl	578:595	Growth of the bacterium occurred at 10-42 °C (optimum, 25-37 °C), at salinities of 0.5-10 % (w/v) NaCl (optimum 3-5 % NaCl) and pH of 6-10 (optimum pH 9).
30207517	4	33	theme	%	590:590	arg1	NaCl					572:575	0.5-10 % (w/v) NaCl	557:575	0.5-10 % (w/v) NaCl (optimum 3-5 % NaCl)	557:596	Growth of the bacterium occurred at 10-42 °C (optimum, 25-37 °C), at salinities of 0.5-10 % (w/v) NaCl (optimum 3-5 % NaCl) and pH of 6-10 (optimum pH 9).
30207517	9	34	theme	strain	1310:1315	arg1	AK72T					1317:1321	strain AK72T	1310:1321	strain AK72T	1310:1321	Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain AK72T was closely related to Bacillus cellulosilyticus (96.5 %) and Bacillus vedderi (96.3 %), but the novel strain AK72T formed a separate clade with Bacillus aurantiacus whereas B. cellulosilyticus and B. vedderi were clustered in a separate clade.
30207517	7	35	theme	major	971:975	arg1	diphosphatidylglycerol					1009:1030	diphosphatidylglycerol	1009:1030	diphosphatidylglycerol	1009:1030	The major polar lipids of the strain were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unidentified aminophospholipid, three unidentified phospholipids and three unidentified lipids.
30207517	7	35	theme	major	971:975	arg1	lipids					983:988	The major polar lipids	967:988	The major polar lipids of the strain	967:1002	The major polar lipids of the strain were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unidentified aminophospholipid, three unidentified phospholipids and three unidentified lipids.
30207517	5	36	theme	Strain	629:634	arg1	AK72T					636:640	Strain AK72T	629:640	Strain AK72T	629:640	Strain AK72T was positive for oxidase, catalase, nitrate reductase, phenylalanine deaminase, ornithine decarboxylase, aesculinase, lipase and urease activities.
30207517	2	37	theme	water	251:255	arg1	sample					257:262	a water sample	249:262	a water sample collected from Sambhar salt lake, Rajasthan, India	249:313	A novel Gram-stain-positive, rod-shaped, motile, spore-forming, strictly aerobic, alkali- and halo- tolerant bacterium, designated strain AK72T, was isolated from a water sample collected from Sambhar salt lake, Rajasthan, India.
30207517	7	38	theme	unidentified	1159:1170	arg1	lipids					1172:1177	three unidentified lipids	1153:1177	three unidentified lipids	1153:1177	The major polar lipids of the strain were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unidentified aminophospholipid, three unidentified phospholipids and three unidentified lipids.
30207517	7	39	theme	polar	977:981	arg1	diphosphatidylglycerol					1009:1030	diphosphatidylglycerol	1009:1030	diphosphatidylglycerol	1009:1030	The major polar lipids of the strain were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unidentified aminophospholipid, three unidentified phospholipids and three unidentified lipids.
30207517	7	39	theme	polar	977:981	arg1	lipids					983:988	The major polar lipids	967:988	The major polar lipids of the strain	967:1002	The major polar lipids of the strain were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unidentified aminophospholipid, three unidentified phospholipids and three unidentified lipids.
30207517	8	40	theme	strain	1215:1220	arg1	AK72T					1222:1226	the strain AK72T	1211:1226	the strain AK72T	1211:1226	The genomic DNA G+C content of the strain AK72T was 36.8 mol%.
30207517	5	41	theme	urease	771:776	arg1	activities					778:787	urease activities	771:787	urease activities	771:787	Strain AK72T was positive for oxidase, catalase, nitrate reductase, phenylalanine deaminase, ornithine decarboxylase, aesculinase, lipase and urease activities.
30207517	9	42	theme	gene	1281:1284	arg1	sequence					1286:1293	the 16S rRNA gene sequence	1268:1293	the 16S rRNA gene sequence	1268:1293	Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain AK72T was closely related to Bacillus cellulosilyticus (96.5 %) and Bacillus vedderi (96.3 %), but the novel strain AK72T formed a separate clade with Bacillus aurantiacus whereas B. cellulosilyticus and B. vedderi were clustered in a separate clade.
30207517	6	43	theme	meso-diaminopimelic	910:928	arg1	acid					961:964	the diagnostic diamino acid	938:964	the diagnostic diamino acid	938:964	The predominant fatty acids were iso-C15 : 0, anteiso-C15 : 0 and iso-C16 : 0 and the cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid.
30207517	6	43	theme	meso-diaminopimelic	910:928	arg1	acid					930:933	meso-diaminopimelic acid	910:933	meso-diaminopimelic acid	910:933	The predominant fatty acids were iso-C15 : 0, anteiso-C15 : 0 and iso-C16 : 0 and the cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid.
30207517	3	44	with	incubation	443:452	arg1	pH					468:469	pH 9	468:471	pH 9	468:471	The colony appears circular, shiny, smooth, translucent or slightly pale in colour and convex with an entire margin after 48 h incubation at 37 °C with pH 9.
30207517	2	45	dep	rod-shaped	115:124	arg1	aerobic					159:165	aerobic	159:165	aerobic	159:165	A novel Gram-stain-positive, rod-shaped, motile, spore-forming, strictly aerobic, alkali- and halo- tolerant bacterium, designated strain AK72T, was isolated from a water sample collected from Sambhar salt lake, Rajasthan, India.
30207517	2	46	theme	spore-forming	135:147	arg1	bacterium					195:203	rod-shaped, motile, spore-forming, strictly aerobic, alkali- and halo- tolerant bacterium	115:203	rod-shaped, motile, spore-forming, strictly aerobic, alkali- and halo- tolerant bacterium	115:203	A novel Gram-stain-positive, rod-shaped, motile, spore-forming, strictly aerobic, alkali- and halo- tolerant bacterium, designated strain AK72T, was isolated from a water sample collected from Sambhar salt lake, Rajasthan, India.
30207517	2	46	theme	spore-forming	135:147	arg1	Gram-stain-positive					94:112	A novel Gram-stain-positive	86:112	A novel Gram-stain-positive	86:112	A novel Gram-stain-positive, rod-shaped, motile, spore-forming, strictly aerobic, alkali- and halo- tolerant bacterium, designated strain AK72T, was isolated from a water sample collected from Sambhar salt lake, Rajasthan, India.
30207517	9	47	theme	novel	1420:1424	arg1	AK72T					1433:1437	the novel strain AK72T	1416:1437	the novel strain AK72T formed a separate clade with Bacillus aurantiacus whereas B. cellulosilyticus and B. vedderi	1416:1530	Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain AK72T was closely related to Bacillus cellulosilyticus (96.5 %) and Bacillus vedderi (96.3 %), but the novel strain AK72T formed a separate clade with Bacillus aurantiacus whereas B. cellulosilyticus and B. vedderi were clustered in a separate clade.
30207517	9	48	theme	sequence	1286:1293	arg1	analysis					1256:1263	Phylogenetic analysis	1243:1263	Phylogenetic analysis of the 16S rRNA gene sequence	1243:1293	Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain AK72T was closely related to Bacillus cellulosilyticus (96.5 %) and Bacillus vedderi (96.3 %), but the novel strain AK72T formed a separate clade with Bacillus aurantiacus whereas B. cellulosilyticus and B. vedderi were clustered in a separate clade.
30207517	2	49	attach	isolated	235:242	arg2	bacterium					195:203	rod-shaped, motile, spore-forming, strictly aerobic, alkali- and halo- tolerant bacterium	115:203	rod-shaped, motile, spore-forming, strictly aerobic, alkali- and halo- tolerant bacterium	115:203	A novel Gram-stain-positive, rod-shaped, motile, spore-forming, strictly aerobic, alkali- and halo- tolerant bacterium, designated strain AK72T, was isolated from a water sample collected from Sambhar salt lake, Rajasthan, India.
30207517	2	49	attach	isolated	235:242	arg1	sample					257:262	a water sample	249:262	a water sample collected from Sambhar salt lake, Rajasthan, India	249:313	A novel Gram-stain-positive, rod-shaped, motile, spore-forming, strictly aerobic, alkali- and halo- tolerant bacterium, designated strain AK72T, was isolated from a water sample collected from Sambhar salt lake, Rajasthan, India.
30207517	2	49	attach	isolated	235:242	arg2	Gram-stain-positive					94:112	A novel Gram-stain-positive	86:112	A novel Gram-stain-positive	86:112	A novel Gram-stain-positive, rod-shaped, motile, spore-forming, strictly aerobic, alkali- and halo- tolerant bacterium, designated strain AK72T, was isolated from a water sample collected from Sambhar salt lake, Rajasthan, India.
30207517	10	50	with	combination	1586:1596	arg1	characteristics					1618:1632	the phenotypic characteristics	1603:1632	the phenotypic characteristics	1603:1632	The above data in combination with the phenotypic characteristics and phylogenetic data inferred that strain AK72T represents a novel species of the genus Bacillus, for which the name Bacillusshivajii sp.
30207517	2	51	theme	tolerant	186:193	arg1	bacterium					195:203	rod-shaped, motile, spore-forming, strictly aerobic, alkali- and halo- tolerant bacterium	115:203	rod-shaped, motile, spore-forming, strictly aerobic, alkali- and halo- tolerant bacterium	115:203	A novel Gram-stain-positive, rod-shaped, motile, spore-forming, strictly aerobic, alkali- and halo- tolerant bacterium, designated strain AK72T, was isolated from a water sample collected from Sambhar salt lake, Rajasthan, India.
30207517	2	51	theme	tolerant	186:193	arg1	Gram-stain-positive					94:112	A novel Gram-stain-positive	86:112	A novel Gram-stain-positive	86:112	A novel Gram-stain-positive, rod-shaped, motile, spore-forming, strictly aerobic, alkali- and halo- tolerant bacterium, designated strain AK72T, was isolated from a water sample collected from Sambhar salt lake, Rajasthan, India.
30207517	12	52	theme	=MTCC	1817:1821	arg1	32183T					1846:1851	=MTCC 12636T=KCTC 33981T=JCM 32183T	1817:1851	=MTCC 12636T=KCTC 33981T=JCM 32183T	1817:1851	The type strain is AK72T (=MTCC 12636T=KCTC 33981T=JCM 32183T).
30207517	12	52	theme	=MTCC	1817:1821	arg1	AK72T					1810:1814	AK72T	1810:1814	AK72T (=MTCC 12636T=KCTC 33981T=JCM 32183T)	1810:1852	The type strain is AK72T (=MTCC 12636T=KCTC 33981T=JCM 32183T).
30207517	7	53	theme	strain	997:1002	arg1	diphosphatidylglycerol					1009:1030	diphosphatidylglycerol	1009:1030	diphosphatidylglycerol	1009:1030	The major polar lipids of the strain were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unidentified aminophospholipid, three unidentified phospholipids and three unidentified lipids.
30207517	7	53	theme	strain	997:1002	arg1	lipids					983:988	The major polar lipids	967:988	The major polar lipids of the strain	967:1002	The major polar lipids of the strain were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unidentified aminophospholipid, three unidentified phospholipids and three unidentified lipids.
30207517	10	54	theme	novel	1696:1700	arg1	species					1702:1708	a novel species	1694:1708	a novel species	1694:1708	The above data in combination with the phenotypic characteristics and phylogenetic data inferred that strain AK72T represents a novel species of the genus Bacillus, for which the name Bacillusshivajii sp.
30207517	2	55	theme	motile	127:132	arg1	bacterium					195:203	rod-shaped, motile, spore-forming, strictly aerobic, alkali- and halo- tolerant bacterium	115:203	rod-shaped, motile, spore-forming, strictly aerobic, alkali- and halo- tolerant bacterium	115:203	A novel Gram-stain-positive, rod-shaped, motile, spore-forming, strictly aerobic, alkali- and halo- tolerant bacterium, designated strain AK72T, was isolated from a water sample collected from Sambhar salt lake, Rajasthan, India.
30207517	2	55	theme	motile	127:132	arg1	Gram-stain-positive					94:112	A novel Gram-stain-positive	86:112	A novel Gram-stain-positive	86:112	A novel Gram-stain-positive, rod-shaped, motile, spore-forming, strictly aerobic, alkali- and halo- tolerant bacterium, designated strain AK72T, was isolated from a water sample collected from Sambhar salt lake, Rajasthan, India.
30207517	2	56	theme	halo-	180:184	arg1	bacterium					195:203	rod-shaped, motile, spore-forming, strictly aerobic, alkali- and halo- tolerant bacterium	115:203	rod-shaped, motile, spore-forming, strictly aerobic, alkali- and halo- tolerant bacterium	115:203	A novel Gram-stain-positive, rod-shaped, motile, spore-forming, strictly aerobic, alkali- and halo- tolerant bacterium, designated strain AK72T, was isolated from a water sample collected from Sambhar salt lake, Rajasthan, India.
30207517	2	56	theme	halo-	180:184	arg1	Gram-stain-positive					94:112	A novel Gram-stain-positive	86:112	A novel Gram-stain-positive	86:112	A novel Gram-stain-positive, rod-shaped, motile, spore-forming, strictly aerobic, alkali- and halo- tolerant bacterium, designated strain AK72T, was isolated from a water sample collected from Sambhar salt lake, Rajasthan, India.
30207517	3	57	from	pale	384:387	arg1	convex					403:408	convex	403:408	convex	403:408	The colony appears circular, shiny, smooth, translucent or slightly pale in colour and convex with an entire margin after 48 h incubation at 37 °C with pH 9.
30207517	3	57	from	pale	384:387	arg1	colour					392:397	colour	392:397	colour	392:397	The colony appears circular, shiny, smooth, translucent or slightly pale in colour and convex with an entire margin after 48 h incubation at 37 °C with pH 9.
30207517	1	58	theme	India	79:83	arg1	sample					50:55	a water sample	42:55	a water sample of Sambhar salt lake, India	42:83	nov., isolated from a water sample of Sambhar salt lake, India.
30207517	12	59	theme	33981T=JCM	1835:1844	arg1	32183T					1846:1851	=MTCC 12636T=KCTC 33981T=JCM 32183T	1817:1851	=MTCC 12636T=KCTC 33981T=JCM 32183T	1817:1851	The type strain is AK72T (=MTCC 12636T=KCTC 33981T=JCM 32183T).
30207517	12	59	theme	33981T=JCM	1835:1844	arg1	AK72T					1810:1814	AK72T	1810:1814	AK72T (=MTCC 12636T=KCTC 33981T=JCM 32183T)	1810:1852	The type strain is AK72T (=MTCC 12636T=KCTC 33981T=JCM 32183T).
30207517	9	60	theme	separate	1552:1559	arg1	clade					1561:1565	a separate clade	1550:1565	a separate clade	1550:1565	Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain AK72T was closely related to Bacillus cellulosilyticus (96.5 %) and Bacillus vedderi (96.3 %), but the novel strain AK72T formed a separate clade with Bacillus aurantiacus whereas B. cellulosilyticus and B. vedderi were clustered in a separate clade.
30207517	2	61	theme	alkali-	168:174	arg1	bacterium					195:203	rod-shaped, motile, spore-forming, strictly aerobic, alkali- and halo- tolerant bacterium	115:203	rod-shaped, motile, spore-forming, strictly aerobic, alkali- and halo- tolerant bacterium	115:203	A novel Gram-stain-positive, rod-shaped, motile, spore-forming, strictly aerobic, alkali- and halo- tolerant bacterium, designated strain AK72T, was isolated from a water sample collected from Sambhar salt lake, Rajasthan, India.
30207517	2	61	theme	alkali-	168:174	arg1	Gram-stain-positive					94:112	A novel Gram-stain-positive	86:112	A novel Gram-stain-positive	86:112	A novel Gram-stain-positive, rod-shaped, motile, spore-forming, strictly aerobic, alkali- and halo- tolerant bacterium, designated strain AK72T, was isolated from a water sample collected from Sambhar salt lake, Rajasthan, India.
30207517	10	62	theme	above	1572:1576	arg1	data					1578:1581	The above data	1568:1581	The above data in combination with the phenotypic characteristics	1568:1632	The above data in combination with the phenotypic characteristics and phylogenetic data inferred that strain AK72T represents a novel species of the genus Bacillus, for which the name Bacillusshivajii sp.
30207517	7	63	theme	unidentified	1122:1133	arg1	phospholipids					1135:1147	three unidentified phospholipids	1116:1147	three unidentified phospholipids	1116:1147	The major polar lipids of the strain were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unidentified aminophospholipid, three unidentified phospholipids and three unidentified lipids.
30207517	4	64	theme	NaCl	572:575	arg1	salinities					543:552	salinities	543:552	salinities of 0.5-10 % (w/v) NaCl (optimum 3-5 % NaCl) and pH of 6-10 (optimum pH 9)	543:626	Growth of the bacterium occurred at 10-42 °C (optimum, 25-37 °C), at salinities of 0.5-10 % (w/v) NaCl (optimum 3-5 % NaCl) and pH of 6-10 (optimum pH 9).
30207517	12	65	theme	type	1795:1798	arg1	AK72T					1810:1814	AK72T	1810:1814	AK72T (=MTCC 12636T=KCTC 33981T=JCM 32183T)	1810:1852	The type strain is AK72T (=MTCC 12636T=KCTC 33981T=JCM 32183T).
30207517	12	65	theme	type	1795:1798	arg1	strain					1800:1805	The type strain	1791:1805	The type strain	1791:1805	The type strain is AK72T (=MTCC 12636T=KCTC 33981T=JCM 32183T).
30207517	10	66	theme	genus	1717:1721	arg1	species					1702:1708	a novel species	1694:1708	a novel species	1694:1708	The above data in combination with the phenotypic characteristics and phylogenetic data inferred that strain AK72T represents a novel species of the genus Bacillus, for which the name Bacillusshivajii sp.
30207517	8	67	theme	AK72T	1222:1226	arg1	G+C content					1196:1206	The genomic DNA G+C content	1180:1206	The genomic DNA G+C content of the strain AK72T	1180:1226	The genomic DNA G+C content of the strain AK72T was 36.8 mol%.
30207517	8	67	theme	AK72T	1222:1226	arg1	%					1240:1240	36.8 mol%	1232:1240	36.8 mol%	1232:1240	The genomic DNA G+C content of the strain AK72T was 36.8 mol%.
30207517	3	68	from	colour	392:397	arg1	pale					384:387	pale	384:387	pale	384:387	The colony appears circular, shiny, smooth, translucent or slightly pale in colour and convex with an entire margin after 48 h incubation at 37 °C with pH 9.
30207517	4	69	theme	3-5 	586:589	arg1	 NaCl					591:595	optimum 3-5 % NaCl	578:595	optimum 3-5 % NaCl	578:595	Growth of the bacterium occurred at 10-42 °C (optimum, 25-37 °C), at salinities of 0.5-10 % (w/v) NaCl (optimum 3-5 % NaCl) and pH of 6-10 (optimum pH 9).
30207517	4	69	theme	3-5 	586:589	arg1	NaCl					572:575	0.5-10 % (w/v) NaCl	557:575	0.5-10 % (w/v) NaCl (optimum 3-5 % NaCl)	557:596	Growth of the bacterium occurred at 10-42 °C (optimum, 25-37 °C), at salinities of 0.5-10 % (w/v) NaCl (optimum 3-5 % NaCl) and pH of 6-10 (optimum pH 9).
30207517	5	70	theme	ornithine	722:730	arg1	decarboxylase					732:744	ornithine decarboxylase	722:744	ornithine decarboxylase	722:744	Strain AK72T was positive for oxidase, catalase, nitrate reductase, phenylalanine deaminase, ornithine decarboxylase, aesculinase, lipase and urease activities.
30207517	2	71	theme	strain	217:222	arg1	AK72T					224:228	strain AK72T	217:228	strain AK72T	217:228	A novel Gram-stain-positive, rod-shaped, motile, spore-forming, strictly aerobic, alkali- and halo- tolerant bacterium, designated strain AK72T, was isolated from a water sample collected from Sambhar salt lake, Rajasthan, India.
30207517	4	72	theme	%	564:564	arg1	 NaCl					591:595	optimum 3-5 % NaCl	578:595	optimum 3-5 % NaCl	578:595	Growth of the bacterium occurred at 10-42 °C (optimum, 25-37 °C), at salinities of 0.5-10 % (w/v) NaCl (optimum 3-5 % NaCl) and pH of 6-10 (optimum pH 9).
30207517	4	72	theme	%	564:564	arg1	NaCl					572:575	0.5-10 % (w/v) NaCl	557:575	0.5-10 % (w/v) NaCl (optimum 3-5 % NaCl)	557:596	Growth of the bacterium occurred at 10-42 °C (optimum, 25-37 °C), at salinities of 0.5-10 % (w/v) NaCl (optimum 3-5 % NaCl) and pH of 6-10 (optimum pH 9).
30207517	6	73	theme	diagnostic	942:951	arg1	acid					961:964	the diagnostic diamino acid	938:964	the diagnostic diamino acid	938:964	The predominant fatty acids were iso-C15 : 0, anteiso-C15 : 0 and iso-C16 : 0 and the cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid.
30207517	6	73	theme	diagnostic	942:951	arg1	acid					930:933	meso-diaminopimelic acid	910:933	meso-diaminopimelic acid	910:933	The predominant fatty acids were iso-C15 : 0, anteiso-C15 : 0 and iso-C16 : 0 and the cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid.
30207517	9	74	theme	Phylogenetic	1243:1254	arg1	analysis					1256:1263	Phylogenetic analysis	1243:1263	Phylogenetic analysis of the 16S rRNA gene sequence	1243:1293	Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain AK72T was closely related to Bacillus cellulosilyticus (96.5 %) and Bacillus vedderi (96.3 %), but the novel strain AK72T formed a separate clade with Bacillus aurantiacus whereas B. cellulosilyticus and B. vedderi were clustered in a separate clade.
30207517	9	75	theme	separate	1448:1455	arg1	clade					1457:1461	a separate clade	1446:1461	a separate clade with Bacillus aurantiacus	1446:1487	Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain AK72T was closely related to Bacillus cellulosilyticus (96.5 %) and Bacillus vedderi (96.3 %), but the novel strain AK72T formed a separate clade with Bacillus aurantiacus whereas B. cellulosilyticus and B. vedderi were clustered in a separate clade.
30207517	5	76	theme	phenylalanine	697:709	arg1	deaminase					711:719	phenylalanine deaminase	697:719	phenylalanine deaminase	697:719	Strain AK72T was positive for oxidase, catalase, nitrate reductase, phenylalanine deaminase, ornithine decarboxylase, aesculinase, lipase and urease activities.
30207517	10	77	from	data	1578:1581	arg1	combination					1586:1596	combination	1586:1596	combination with the phenotypic characteristics	1586:1632	The above data in combination with the phenotypic characteristics and phylogenetic data inferred that strain AK72T represents a novel species of the genus Bacillus, for which the name Bacillusshivajii sp.
30207517	9	78	with	clade	1457:1461	arg1	aurantiacus					1477:1487	Bacillus aurantiacus	1468:1487	Bacillus aurantiacus	1468:1487	Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain AK72T was closely related to Bacillus cellulosilyticus (96.5 %) and Bacillus vedderi (96.3 %), but the novel strain AK72T formed a separate clade with Bacillus aurantiacus whereas B. cellulosilyticus and B. vedderi were clustered in a separate clade.
30207517	2	79	theme	novel	88:92	arg1	bacterium					195:203	rod-shaped, motile, spore-forming, strictly aerobic, alkali- and halo- tolerant bacterium	115:203	rod-shaped, motile, spore-forming, strictly aerobic, alkali- and halo- tolerant bacterium	115:203	A novel Gram-stain-positive, rod-shaped, motile, spore-forming, strictly aerobic, alkali- and halo- tolerant bacterium, designated strain AK72T, was isolated from a water sample collected from Sambhar salt lake, Rajasthan, India.
30207517	2	79	theme	novel	88:92	arg1	Gram-stain-positive					94:112	A novel Gram-stain-positive	86:112	A novel Gram-stain-positive	86:112	A novel Gram-stain-positive, rod-shaped, motile, spore-forming, strictly aerobic, alkali- and halo- tolerant bacterium, designated strain AK72T, was isolated from a water sample collected from Sambhar salt lake, Rajasthan, India.
30207517	10	80	dep	genus	1717:1721	arg1	Bacillus					1723:1730	the genus Bacillus	1713:1730	the genus Bacillus	1713:1730	The above data in combination with the phenotypic characteristics and phylogenetic data inferred that strain AK72T represents a novel species of the genus Bacillus, for which the name Bacillusshivajii sp.
30207517	4	81	dep	optimum	520:526	arg1	25-37 °C					529:536	25-37 °C	529:536	25-37 °C	529:536	Growth of the bacterium occurred at 10-42 °C (optimum, 25-37 °C), at salinities of 0.5-10 % (w/v) NaCl (optimum 3-5 % NaCl) and pH of 6-10 (optimum pH 9).
30207517	3	82	theme	48 h	438:441	arg1	incubation					443:452	48 h incubation	438:452	48 h incubation at 37 °C with pH 9	438:471	The colony appears circular, shiny, smooth, translucent or slightly pale in colour and convex with an entire margin after 48 h incubation at 37 °C with pH 9.
30207517	10	83	theme	strain	1670:1675	arg1	AK72T					1677:1681	strain AK72T	1670:1681	strain AK72T	1670:1681	The above data in combination with the phenotypic characteristics and phylogenetic data inferred that strain AK72T represents a novel species of the genus Bacillus, for which the name Bacillusshivajii sp.
31283757	1	0	from	damage	298:303	arg1	disorders					319:327	autoimmune disorders	308:327	autoimmune disorders	308:327	Neutrophil extracellular traps (NET) formation is part of the neutrophil response to infections, but excessive or inappropriate NETosis may trigger the production of autoantibodies and cause organ damage in autoimmune disorders.
31283757	3	1	dep	analysis	638:645	arg1	means					619:623	means	619:623	means	619:623	In the present work, the protein composition and post-translational modifications of NET produced under different stimuli have been studied by means of proteomic analysis.
31283757	5	2	theme	unsupervised	876:887	arg1	analyses					913:920	unsupervised hierarchical clustering analyses	876:920	unsupervised hierarchical clustering analyses	876:920	Data were analyzed by unsupervised hierarchical clustering analyses.
31283757	9	3	theme	composition	1436:1446	arg1	terms					1414:1418	terms	1414:1418	terms of both protein composition and post-translational modifications	1414:1483	Thus, proteomic analysis indicates that NETs induced by different stimuli are heterogeneous in terms of both protein composition and post-translational modifications, suggesting that NET induced in different conditions may have different biological effects.
31283757	7	4	theme	PMA-	1234:1237	arg1	NETs					1255:1258	PMA- and LPS-induced NETs	1234:1258	PMA- and LPS-induced NETs	1234:1258	Among the post-translational modifications we examined, methionine sulfoxidation is frequent especially in PMA- and LPS-induced NETs.
31283757	6	5	dep	source	971:976	arg1	any					967:969	any	967:969	any	967:969	We identified proteins contained in NETs of any source or exclusive of one stimulus: LPS-induced and spontaneous NET diverge in protein composition, while PMA- and A23187-induced NET appear more similar.
31283757	7	6	from	NETs	1255:1258	arg1	frequent					1211:1218	frequent	1211:1218	frequent	1211:1218	Among the post-translational modifications we examined, methionine sulfoxidation is frequent especially in PMA- and LPS-induced NETs.
31283757	2	7	theme	netting	344:350	arg1	neutrophils					352:362	Spontaneously netting neutrophils	330:362	Spontaneously netting neutrophils	330:362	Spontaneously netting neutrophils are not frequent and induction of NET in vitro by selected stimuli is necessary to investigate their structure.
31283757	9	8	from	heterogeneous	1397:1409	arg1	terms					1414:1418	terms	1414:1418	terms of both protein composition and post-translational modifications	1414:1483	Thus, proteomic analysis indicates that NETs induced by different stimuli are heterogeneous in terms of both protein composition and post-translational modifications, suggesting that NET induced in different conditions may have different biological effects.
31283757	0	9	theme	proteomic	81:89	arg1	analysis					91:98	A comparative proteomic analysis	67:98	A comparative proteomic analysis	67:98	Neutrophil extracellular traps (NET) induced by different stimuli: A comparative proteomic analysis.
31283757	3	10	theme	post-translational	525:542	arg1	modifications					544:556	post-translational modifications	525:556	post-translational modifications	525:556	In the present work, the protein composition and post-translational modifications of NET produced under different stimuli have been studied by means of proteomic analysis.
31283757	6	11	theme	exclusive	981:989	arg1	NETs					959:962	NETs	959:962	NETs of any source or exclusive of one stimulus	959:1005	We identified proteins contained in NETs of any source or exclusive of one stimulus: LPS-induced and spontaneous NET diverge in protein composition, while PMA- and A23187-induced NET appear more similar.
31283757	0	12	theme	comparative	69:79	arg1	analysis					91:98	A comparative proteomic analysis	67:98	A comparative proteomic analysis	67:98	Neutrophil extracellular traps (NET) induced by different stimuli: A comparative proteomic analysis.
31283757	9	13	theme	proteomic	1325:1333	arg1	analysis					1335:1342	proteomic analysis	1325:1342	proteomic analysis	1325:1342	Thus, proteomic analysis indicates that NETs induced by different stimuli are heterogeneous in terms of both protein composition and post-translational modifications, suggesting that NET induced in different conditions may have different biological effects.
31283757	0	14	dep	analysis	91:98	arg1	Neutrophil					0:9	Neutrophil extracellular traps	0:29	Neutrophil extracellular traps (NET) induced by different stimuli	0:64	Neutrophil extracellular traps (NET) induced by different stimuli: A comparative proteomic analysis.
31283757	6	15	theme	stimulus	998:1005	arg1	source					971:976	source	971:976	source	971:976	We identified proteins contained in NETs of any source or exclusive of one stimulus: LPS-induced and spontaneous NET diverge in protein composition, while PMA- and A23187-induced NET appear more similar.
31283757	3	16	dep	composition	509:519	arg1	the					497:499	the	497:499	the	497:499	In the present work, the protein composition and post-translational modifications of NET produced under different stimuli have been studied by means of proteomic analysis.
31283757	4	17	from	donors	673:678	arg1	Neutrophils					648:658	Neutrophils	648:658	Neutrophils from healthy donors	648:678	Neutrophils from healthy donors were stimulated by PMA, A23187, Escherichia coli LPS or untreated; after three hours, cells were washed, treated with DNase and supernatants collected for mass spectrometry.
31283757	4	18	theme	healthy	665:671	arg1	donors					673:678	healthy donors	665:678	healthy donors	665:678	Neutrophils from healthy donors were stimulated by PMA, A23187, Escherichia coli LPS or untreated; after three hours, cells were washed, treated with DNase and supernatants collected for mass spectrometry.
31283757	9	19	theme	modifications	1471:1483	arg1	terms					1414:1418	terms	1414:1418	terms of both protein composition and post-translational modifications	1414:1483	Thus, proteomic analysis indicates that NETs induced by different stimuli are heterogeneous in terms of both protein composition and post-translational modifications, suggesting that NET induced in different conditions may have different biological effects.
31283757	5	20	theme	hierarchical	889:900	arg1	analyses					913:920	unsupervised hierarchical clustering analyses	876:920	unsupervised hierarchical clustering analyses	876:920	Data were analyzed by unsupervised hierarchical clustering analyses.
31283757	1	21	dep	extracellular	112:124	arg1	traps					126:130	traps	126:130	traps	126:130	Neutrophil extracellular traps (NET) formation is part of the neutrophil response to infections, but excessive or inappropriate NETosis may trigger the production of autoantibodies and cause organ damage in autoimmune disorders.
31283757	1	21	dep	extracellular	112:124	arg1	NET					133:135	NET	133:135	NET	133:135	Neutrophil extracellular traps (NET) formation is part of the neutrophil response to infections, but excessive or inappropriate NETosis may trigger the production of autoantibodies and cause organ damage in autoimmune disorders.
31283757	4	22	dep	washed	777:782	arg1	treated					785:791	treated	785:791	treated with DNase and supernatants collected for mass spectrometry	785:851	Neutrophils from healthy donors were stimulated by PMA, A23187, Escherichia coli LPS or untreated; after three hours, cells were washed, treated with DNase and supernatants collected for mass spectrometry.
31283757	0	23	theme	extracellular	11:23	arg1	Neutrophil					0:9	Neutrophil extracellular traps	0:29	Neutrophil extracellular traps (NET) induced by different stimuli	0:64	Neutrophil extracellular traps (NET) induced by different stimuli: A comparative proteomic analysis.
31283757	9	24	theme	different	1375:1383	arg1	stimuli					1385:1391	different stimuli	1375:1391	different stimuli	1375:1391	Thus, proteomic analysis indicates that NETs induced by different stimuli are heterogeneous in terms of both protein composition and post-translational modifications, suggesting that NET induced in different conditions may have different biological effects.
31283757	4	25	theme	mass	835:838	arg1	spectrometry					840:851	mass spectrometry	835:851	mass spectrometry	835:851	Neutrophils from healthy donors were stimulated by PMA, A23187, Escherichia coli LPS or untreated; after three hours, cells were washed, treated with DNase and supernatants collected for mass spectrometry.
31283757	7	26	from	frequent	1211:1218	arg1	NETs					1255:1258	PMA- and LPS-induced NETs	1234:1258	PMA- and LPS-induced NETs	1234:1258	Among the post-translational modifications we examined, methionine sulfoxidation is frequent especially in PMA- and LPS-induced NETs.
31283757	7	27	theme	methionine	1183:1192	arg1	sulfoxidation					1194:1206	methionine sulfoxidation	1183:1206	methionine sulfoxidation	1183:1206	Among the post-translational modifications we examined, methionine sulfoxidation is frequent especially in PMA- and LPS-induced NETs.
31283757	6	28	theme	source	971:976	arg1	NETs					959:962	NETs	959:962	NETs of any source or exclusive of one stimulus	959:1005	We identified proteins contained in NETs of any source or exclusive of one stimulus: LPS-induced and spontaneous NET diverge in protein composition, while PMA- and A23187-induced NET appear more similar.
31283757	7	29	dep	modifications	1156:1168	arg1	we					1170:1171	we	1170:1171	we	1170:1171	Among the post-translational modifications we examined, methionine sulfoxidation is frequent especially in PMA- and LPS-induced NETs.
31283757	9	30	theme	post-translational	1452:1469	arg1	modifications					1471:1483	post-translational modifications	1452:1483	post-translational modifications	1452:1483	Thus, proteomic analysis indicates that NETs induced by different stimuli are heterogeneous in terms of both protein composition and post-translational modifications, suggesting that NET induced in different conditions may have different biological effects.
31283757	4	31	dep	Escherichia	712:722	arg1	coli					724:727	coli	724:727	coli	724:727	Neutrophils from healthy donors were stimulated by PMA, A23187, Escherichia coli LPS or untreated; after three hours, cells were washed, treated with DNase and supernatants collected for mass spectrometry.
31283757	1	32	theme	neutrophil	163:172	arg1	response					174:181	the neutrophil response	159:181	the neutrophil response to infections	159:195	Neutrophil extracellular traps (NET) formation is part of the neutrophil response to infections, but excessive or inappropriate NETosis may trigger the production of autoantibodies and cause organ damage in autoimmune disorders.
31283757	1	33	theme	response	174:181	arg1	part					151:154	part	151:154	part of the neutrophil response to infections	151:195	Neutrophil extracellular traps (NET) formation is part of the neutrophil response to infections, but excessive or inappropriate NETosis may trigger the production of autoantibodies and cause organ damage in autoimmune disorders.
31283757	2	34	theme	selected	414:421	arg1	stimuli					423:429	selected stimuli	414:429	selected stimuli	414:429	Spontaneously netting neutrophils are not frequent and induction of NET in vitro by selected stimuli is necessary to investigate their structure.
31283757	1	35	theme	autoantibodies	267:280	arg1	production					253:262	the production	249:262	the production of autoantibodies	249:280	Neutrophil extracellular traps (NET) formation is part of the neutrophil response to infections, but excessive or inappropriate NETosis may trigger the production of autoantibodies and cause organ damage in autoimmune disorders.
31283757	3	36	theme	NET	561:563	arg1	composition					509:519	protein composition	501:519	protein composition	501:519	In the present work, the protein composition and post-translational modifications of NET produced under different stimuli have been studied by means of proteomic analysis.
31283757	3	36	theme	NET	561:563	arg1	modifications					544:556	post-translational modifications	525:556	post-translational modifications	525:556	In the present work, the protein composition and post-translational modifications of NET produced under different stimuli have been studied by means of proteomic analysis.
31283757	7	37	theme	post-translational	1137:1154	arg1	modifications					1156:1168	the post-translational modifications	1133:1168	the post-translational modifications we examined	1133:1180	Among the post-translational modifications we examined, methionine sulfoxidation is frequent especially in PMA- and LPS-induced NETs.
31283757	3	38	theme	present	483:489	arg1	work					491:494	the present work	479:494	the present work	479:494	In the present work, the protein composition and post-translational modifications of NET produced under different stimuli have been studied by means of proteomic analysis.
31283757	9	39	contain	have	1542:1545	arg1	NET					1502:1504	NET	1502:1504	NET	1502:1504	Thus, proteomic analysis indicates that NETs induced by different stimuli are heterogeneous in terms of both protein composition and post-translational modifications, suggesting that NET induced in different conditions may have different biological effects.
31283757	9	39	contain	have	1542:1545	arg2	effects					1568:1574	different biological effects	1547:1574	different biological effects	1547:1574	Thus, proteomic analysis indicates that NETs induced by different stimuli are heterogeneous in terms of both protein composition and post-translational modifications, suggesting that NET induced in different conditions may have different biological effects.
31283757	6	40	theme	protein	1051:1057	arg1	composition					1059:1069	protein composition	1051:1069	protein composition	1051:1069	We identified proteins contained in NETs of any source or exclusive of one stimulus: LPS-induced and spontaneous NET diverge in protein composition, while PMA- and A23187-induced NET appear more similar.
31283757	9	41	theme	different	1517:1525	arg1	conditions					1527:1536	different conditions	1517:1536	different conditions	1517:1536	Thus, proteomic analysis indicates that NETs induced by different stimuli are heterogeneous in terms of both protein composition and post-translational modifications, suggesting that NET induced in different conditions may have different biological effects.
31283757	3	42	theme	proteomic	628:636	arg1	analysis					638:645	proteomic analysis	628:645	proteomic analysis	628:645	In the present work, the protein composition and post-translational modifications of NET produced under different stimuli have been studied by means of proteomic analysis.
31283757	2	43	theme	NET	398:400	arg1	induction					385:393	induction	385:393	induction of NET in vitro by selected stimuli	385:429	Spontaneously netting neutrophils are not frequent and induction of NET in vitro by selected stimuli is necessary to investigate their structure.
31283757	1	44	theme	organ	292:296	arg1	damage					298:303	organ damage	292:303	organ damage in autoimmune disorders	292:327	Neutrophil extracellular traps (NET) formation is part of the neutrophil response to infections, but excessive or inappropriate NETosis may trigger the production of autoantibodies and cause organ damage in autoimmune disorders.
31283757	7	45	theme	LPS-induced	1243:1253	arg1	NETs					1255:1258	PMA- and LPS-induced NETs	1234:1258	PMA- and LPS-induced NETs	1234:1258	Among the post-translational modifications we examined, methionine sulfoxidation is frequent especially in PMA- and LPS-induced NETs.
31283757	3	46	theme	different	580:588	arg1	stimuli					590:596	different stimuli	580:596	different stimuli	580:596	In the present work, the protein composition and post-translational modifications of NET produced under different stimuli have been studied by means of proteomic analysis.
31283757	1	47	theme	Neutrophil	101:110	arg1	formation					138:146	Neutrophil extracellular traps (NET) formation	101:146	Neutrophil extracellular traps (NET) formation	101:146	Neutrophil extracellular traps (NET) formation is part of the neutrophil response to infections, but excessive or inappropriate NETosis may trigger the production of autoantibodies and cause organ damage in autoimmune disorders.
31283757	9	48	theme	biological	1557:1566	arg1	effects					1568:1574	different biological effects	1547:1574	different biological effects	1547:1574	Thus, proteomic analysis indicates that NETs induced by different stimuli are heterogeneous in terms of both protein composition and post-translational modifications, suggesting that NET induced in different conditions may have different biological effects.
31283757	1	49	theme	extracellular	112:124	arg1	formation					138:146	Neutrophil extracellular traps (NET) formation	101:146	Neutrophil extracellular traps (NET) formation	101:146	Neutrophil extracellular traps (NET) formation is part of the neutrophil response to infections, but excessive or inappropriate NETosis may trigger the production of autoantibodies and cause organ damage in autoimmune disorders.
31283757	9	50	from	terms	1414:1418	arg1	heterogeneous					1397:1409	heterogeneous	1397:1409	heterogeneous	1397:1409	Thus, proteomic analysis indicates that NETs induced by different stimuli are heterogeneous in terms of both protein composition and post-translational modifications, suggesting that NET induced in different conditions may have different biological effects.
31283757	9	51	theme	different	1547:1555	arg1	effects					1568:1574	different biological effects	1547:1574	different biological effects	1547:1574	Thus, proteomic analysis indicates that NETs induced by different stimuli are heterogeneous in terms of both protein composition and post-translational modifications, suggesting that NET induced in different conditions may have different biological effects.
31283757	5	52	theme	clustering	902:911	arg1	analyses					913:920	unsupervised hierarchical clustering analyses	876:920	unsupervised hierarchical clustering analyses	876:920	Data were analyzed by unsupervised hierarchical clustering analyses.
31283757	4	53	theme	Escherichia	712:722	arg1	LPS					729:731	Escherichia coli LPS	712:731	Escherichia coli LPS	712:731	Neutrophils from healthy donors were stimulated by PMA, A23187, Escherichia coli LPS or untreated; after three hours, cells were washed, treated with DNase and supernatants collected for mass spectrometry.
31283757	1	54	theme	excessive	202:210	arg1	NETosis					229:235	excessive or inappropriate NETosis	202:235	excessive or inappropriate NETosis	202:235	Neutrophil extracellular traps (NET) formation is part of the neutrophil response to infections, but excessive or inappropriate NETosis may trigger the production of autoantibodies and cause organ damage in autoimmune disorders.
31283757	0	55	theme	different	48:56	arg1	stimuli					58:64	different stimuli	48:64	different stimuli	48:64	Neutrophil extracellular traps (NET) induced by different stimuli: A comparative proteomic analysis.
31283757	1	56	theme	autoimmune	308:317	arg1	disorders					319:327	autoimmune disorders	308:327	autoimmune disorders	308:327	Neutrophil extracellular traps (NET) formation is part of the neutrophil response to infections, but excessive or inappropriate NETosis may trigger the production of autoantibodies and cause organ damage in autoimmune disorders.
31283757	0	57	dep	Neutrophil	0:9	arg1	traps					25:29	traps	25:29	traps	25:29	Neutrophil extracellular traps (NET) induced by different stimuli: A comparative proteomic analysis.
31283757	0	57	dep	Neutrophil	0:9	arg1	NET					32:34	NET	32:34	NET	32:34	Neutrophil extracellular traps (NET) induced by different stimuli: A comparative proteomic analysis.
31283757	3	58	theme	protein	501:507	arg1	composition					509:519	protein composition	501:519	protein composition	501:519	In the present work, the protein composition and post-translational modifications of NET produced under different stimuli have been studied by means of proteomic analysis.
31283757	9	59	theme	protein	1428:1434	arg1	composition					1436:1446	protein composition	1428:1446	protein composition	1428:1446	Thus, proteomic analysis indicates that NETs induced by different stimuli are heterogeneous in terms of both protein composition and post-translational modifications, suggesting that NET induced in different conditions may have different biological effects.
31283757	1	60	theme	inappropriate	215:227	arg1	NETosis					229:235	excessive or inappropriate NETosis	202:235	excessive or inappropriate NETosis	202:235	Neutrophil extracellular traps (NET) formation is part of the neutrophil response to infections, but excessive or inappropriate NETosis may trigger the production of autoantibodies and cause organ damage in autoimmune disorders.
29928979	0	0	theme	polymeric	77:85	arg1	films					87:91	polymeric films	77:91	polymeric films	77:91	Investigation of in vitro hydrophilic and hydrophobic dual drug release from polymeric films produced by sodium alginate-MaterBi® drying emulsions.
29928979	10	1	theme	fibroblast	1555:1564	arg1	cells					1573:1577	human dermal fibroblast, adult cells	1542:1577	cells	1573:1577	Films demonstrate excellent cell biocompatibility against human dermal fibroblast, adult cells.
29928979	7	2	theme	model	990:994	arg1	drugs					996:1000	two model drugs	986:1000	two model drugs	986:1000	The films were loaded with two model drugs, a hydrophilic eosin-based cutaneous antiseptic and the hydrophobic curcumin.
29928979	7	2	theme	model	990:994	arg1	antiseptic					1039:1048	antiseptic	1039:1048	antiseptic	1039:1048	The films were loaded with two model drugs, a hydrophilic eosin-based cutaneous antiseptic and the hydrophobic curcumin.
29928979	7	2	theme	model	990:994	arg1	curcumin					1070:1077	the hydrophobic curcumin	1054:1077	the hydrophobic curcumin	1054:1077	The films were loaded with two model drugs, a hydrophilic eosin-based cutaneous antiseptic and the hydrophobic curcumin.
29928979	3	3	theme	water	528:532	arg1	phases					534:539	the oil and water phases	516:539	the oil and water phases	516:539	However, emulsions containing dissolved biopolymers both in the oil and water phases are very scarce.
29928979	8	4	from	fraction	1227:1234	arg1	films					1243:1247	the films	1239:1247	the films	1239:1247	Drug release capacity of the films was investigated in detail, and controlled release of each model drug was achieved either by tuning the polymer fraction in the films during emulsification or by crosslinking sodium alginate fraction of the films by calcium salt solution immersion.
29928979	8	5	theme	controlled	1147:1156	arg1	release					1158:1164	controlled release	1147:1164	controlled release of each model drug	1147:1183	Drug release capacity of the films was investigated in detail, and controlled release of each model drug was achieved either by tuning the polymer fraction in the films during emulsification or by crosslinking sodium alginate fraction of the films by calcium salt solution immersion.
29928979	1	6	dep	in	292:293	arg1	controlled					300:309	controlled	300:309	controlled	300:309	Emulsions are known to be effective carriers of hydrophobic drugs, and particularly injectable emulsions have been successfully implemented for in vivo controlled drug release.
29928979	1	6	dep	in	292:293	arg1	vivo					295:298	vivo	295:298	vivo	295:298	Emulsions are known to be effective carriers of hydrophobic drugs, and particularly injectable emulsions have been successfully implemented for in vivo controlled drug release.
29928979	2	7	theme	pharmaceutical	427:440	arg1	applications					442:453	pharmaceutical applications	427:453	pharmaceutical applications	427:453	Recently, high internal phase emulsions have also been used to produce porous polymeric templates for pharmaceutical applications.
29928979	9	8	theme	single	1414:1419	arg1	drug					1427:1430	a single model drug	1412:1430	a single model drug	1412:1430	The emulsions can be formulated to carry either a single model drug or both drugs depending on the desired application.
29928979	0	9	from	Investigation	0:12	arg1	films					87:91	polymeric films	77:91	polymeric films	77:91	Investigation of in vitro hydrophilic and hydrophobic dual drug release from polymeric films produced by sodium alginate-MaterBi® drying emulsions.
29928979	10	10	theme	excellent	1502:1510	arg1	biocompatibility					1517:1532	excellent cell biocompatibility	1502:1532	excellent cell biocompatibility against human dermal fibroblast, adult cells	1502:1577	Films demonstrate excellent cell biocompatibility against human dermal fibroblast, adult cells.
29928979	8	11	theme	polymer	1219:1225	arg1	fraction					1227:1234	the polymer fraction	1215:1234	the polymer fraction in the films	1215:1247	Drug release capacity of the films was investigated in detail, and controlled release of each model drug was achieved either by tuning the polymer fraction in the films during emulsification or by crosslinking sodium alginate fraction of the films by calcium salt solution immersion.
29928979	8	12	theme	solution	1344:1351	arg1	immersion					1353:1361	calcium salt solution immersion	1331:1361	calcium salt solution immersion	1331:1361	Drug release capacity of the films was investigated in detail, and controlled release of each model drug was achieved either by tuning the polymer fraction in the films during emulsification or by crosslinking sodium alginate fraction of the films by calcium salt solution immersion.
29928979	9	13	theme	model	1421:1425	arg1	drug					1427:1430	a single model drug	1412:1430	a single model drug	1412:1430	The emulsions can be formulated to carry either a single model drug or both drugs depending on the desired application.
29928979	6	14	theme	solid	905:909	arg1	films					911:915	composite solid films	895:915	composite solid films with varying MaterBi®/alginate fractions	895:956	The emulsions were stable for several days and were dried into composite solid films with varying MaterBi®/alginate fractions.
29928979	6	15	theme	composite	895:903	arg1	films					911:915	composite solid films	895:915	composite solid films with varying MaterBi®/alginate fractions	895:956	The emulsions were stable for several days and were dried into composite solid films with varying MaterBi®/alginate fractions.
29928979	8	16	theme	salt	1339:1342	arg1	solution					1344:1351	calcium salt solution	1331:1351	calcium salt solution immersion	1331:1361	Drug release capacity of the films was investigated in detail, and controlled release of each model drug was achieved either by tuning the polymer fraction in the films during emulsification or by crosslinking sodium alginate fraction of the films by calcium salt solution immersion.
29928979	4	17	theme	water	693:697	arg1	dispersion					732:741	aqueous sodium alginate dispersion	708:741	aqueous sodium alginate dispersion	708:741	In this study, we demonstrate such an emulsion, in which the oil phase contains a hydrophobic biodegradable polymer, MaterBi®, and the water phase is aqueous sodium alginate dispersion.
29928979	4	17	theme	water	693:697	arg1	phase					699:703	the water phase	689:703	the water phase	689:703	In this study, we demonstrate such an emulsion, in which the oil phase contains a hydrophobic biodegradable polymer, MaterBi®, and the water phase is aqueous sodium alginate dispersion.
29928979	4	18	theme	aqueous	708:714	arg1	dispersion					732:741	aqueous sodium alginate dispersion	708:741	aqueous sodium alginate dispersion	708:741	In this study, we demonstrate such an emulsion, in which the oil phase contains a hydrophobic biodegradable polymer, MaterBi®, and the water phase is aqueous sodium alginate dispersion.
29928979	4	18	theme	aqueous	708:714	arg1	phase					699:703	the water phase	689:703	the water phase	689:703	In this study, we demonstrate such an emulsion, in which the oil phase contains a hydrophobic biodegradable polymer, MaterBi®, and the water phase is aqueous sodium alginate dispersion.
29928979	0	19	theme	sodium	105:110	arg1	emulsions					137:145	sodium alginate-MaterBi® drying emulsions	105:145	sodium alginate-MaterBi® drying emulsions	105:145	Investigation of in vitro hydrophilic and hydrophobic dual drug release from polymeric films produced by sodium alginate-MaterBi® drying emulsions.
29928979	6	20	with	films	911:915	arg1	fractions					948:956	varying MaterBi®/alginate fractions	922:956	varying MaterBi®/alginate fractions	922:956	The emulsions were stable for several days and were dried into composite solid films with varying MaterBi®/alginate fractions.
29928979	8	21	theme	drug	1180:1183	arg1	release					1158:1164	controlled release	1147:1164	controlled release of each model drug	1147:1183	Drug release capacity of the films was investigated in detail, and controlled release of each model drug was achieved either by tuning the polymer fraction in the films during emulsification or by crosslinking sodium alginate fraction of the films by calcium salt solution immersion.
29928979	2	22	used	used	380:383	arg2	emulsions					355:363	high internal phase emulsions	335:363	high internal phase emulsions	335:363	Recently, high internal phase emulsions have also been used to produce porous polymeric templates for pharmaceutical applications.
29928979	2	23	theme	polymeric	403:411	arg1	templates					413:421	porous polymeric templates	396:421	porous polymeric templates for pharmaceutical applications	396:453	Recently, high internal phase emulsions have also been used to produce porous polymeric templates for pharmaceutical applications.
29928979	8	24	theme	model	1174:1178	arg1	drug					1180:1183	each model drug	1169:1183	each model drug	1169:1183	Drug release capacity of the films was investigated in detail, and controlled release of each model drug was achieved either by tuning the polymer fraction in the films during emulsification or by crosslinking sodium alginate fraction of the films by calcium salt solution immersion.
29928979	8	25	theme	alginate	1297:1304	arg1	fraction					1306:1313	sodium alginate fraction	1290:1313	sodium alginate fraction of the films	1290:1326	Drug release capacity of the films was investigated in detail, and controlled release of each model drug was achieved either by tuning the polymer fraction in the films during emulsification or by crosslinking sodium alginate fraction of the films by calcium salt solution immersion.
29928979	10	26	theme	adult	1567:1571	arg1	cells					1573:1577	human dermal fibroblast, adult cells	1542:1577	cells	1573:1577	Films demonstrate excellent cell biocompatibility against human dermal fibroblast, adult cells.
29928979	1	27	theme	effective	174:182	arg1	carriers					184:191	effective carriers	174:191	effective carriers of hydrophobic drugs	174:212	Emulsions are known to be effective carriers of hydrophobic drugs, and particularly injectable emulsions have been successfully implemented for in vivo controlled drug release.
29928979	10	28	theme	dermal	1548:1553	arg1	cells					1573:1577	human dermal fibroblast, adult cells	1542:1577	cells	1573:1577	Films demonstrate excellent cell biocompatibility against human dermal fibroblast, adult cells.
29928979	8	29	theme	release	1085:1091	arg1	capacity					1093:1100	Drug release capacity	1080:1100	Drug release capacity of the films	1080:1113	Drug release capacity of the films was investigated in detail, and controlled release of each model drug was achieved either by tuning the polymer fraction in the films during emulsification or by crosslinking sodium alginate fraction of the films by calcium salt solution immersion.
29928979	4	30	theme	biodegradable	652:664	arg1	polymer					666:672	a hydrophobic biodegradable polymer	638:672	a hydrophobic biodegradable polymer	638:672	In this study, we demonstrate such an emulsion, in which the oil phase contains a hydrophobic biodegradable polymer, MaterBi®, and the water phase is aqueous sodium alginate dispersion.
29928979	4	30	theme	biodegradable	652:664	arg1	MaterBi®					675:682	MaterBi®	675:682	MaterBi®	675:682	In this study, we demonstrate such an emulsion, in which the oil phase contains a hydrophobic biodegradable polymer, MaterBi®, and the water phase is aqueous sodium alginate dispersion.
29928979	1	31	theme	in	292:293	arg1	release					316:322	in vivo controlled drug release	292:322	in vivo controlled drug release	292:322	Emulsions are known to be effective carriers of hydrophobic drugs, and particularly injectable emulsions have been successfully implemented for in vivo controlled drug release.
29928979	8	32	theme	Drug	1080:1083	arg1	capacity					1093:1100	Drug release capacity	1080:1100	Drug release capacity of the films	1080:1113	Drug release capacity of the films was investigated in detail, and controlled release of each model drug was achieved either by tuning the polymer fraction in the films during emulsification or by crosslinking sodium alginate fraction of the films by calcium salt solution immersion.
29928979	3	33	dep	phases	534:539	arg1	both					508:511	both	508:511	both	508:511	However, emulsions containing dissolved biopolymers both in the oil and water phases are very scarce.
29928979	4	34	theme	alginate	723:730	arg1	dispersion					732:741	aqueous sodium alginate dispersion	708:741	aqueous sodium alginate dispersion	708:741	In this study, we demonstrate such an emulsion, in which the oil phase contains a hydrophobic biodegradable polymer, MaterBi®, and the water phase is aqueous sodium alginate dispersion.
29928979	4	34	theme	alginate	723:730	arg1	phase					699:703	the water phase	689:703	the water phase	689:703	In this study, we demonstrate such an emulsion, in which the oil phase contains a hydrophobic biodegradable polymer, MaterBi®, and the water phase is aqueous sodium alginate dispersion.
29928979	0	35	theme	drying	130:135	arg1	emulsions					137:145	sodium alginate-MaterBi® drying emulsions	105:145	sodium alginate-MaterBi® drying emulsions	105:145	Investigation of in vitro hydrophilic and hydrophobic dual drug release from polymeric films produced by sodium alginate-MaterBi® drying emulsions.
29928979	5	36	theme	ultrasonic	785:794	arg1	processing					796:805	ultrasonic processing	785:805	ultrasonic processing	785:805	The two phases were emulsified simply by ultrasonic processing without any surfactants.
29928979	1	37	theme	hydrophobic	196:206	arg1	drugs					208:212	hydrophobic drugs	196:212	hydrophobic drugs	196:212	Emulsions are known to be effective carriers of hydrophobic drugs, and particularly injectable emulsions have been successfully implemented for in vivo controlled drug release.
29928979	0	38	theme	in	17:18	arg1	release					64:70	in vitro hydrophilic and hydrophobic dual drug release	17:70	in vitro hydrophilic and hydrophobic dual drug release from polymeric films	17:91	Investigation of in vitro hydrophilic and hydrophobic dual drug release from polymeric films produced by sodium alginate-MaterBi® drying emulsions.
29928979	0	39	theme	alginate-MaterBi®	112:128	arg1	emulsions					137:145	sodium alginate-MaterBi® drying emulsions	105:145	sodium alginate-MaterBi® drying emulsions	105:145	Investigation of in vitro hydrophilic and hydrophobic dual drug release from polymeric films produced by sodium alginate-MaterBi® drying emulsions.
29928979	1	40	theme	drugs	208:212	arg1	carriers					184:191	effective carriers	174:191	effective carriers of hydrophobic drugs	174:212	Emulsions are known to be effective carriers of hydrophobic drugs, and particularly injectable emulsions have been successfully implemented for in vivo controlled drug release.
29928979	1	41	theme	drug	311:314	arg1	release					316:322	in vivo controlled drug release	292:322	in vivo controlled drug release	292:322	Emulsions are known to be effective carriers of hydrophobic drugs, and particularly injectable emulsions have been successfully implemented for in vivo controlled drug release.
29928979	8	42	theme	films	1109:1113	arg1	capacity					1093:1100	Drug release capacity	1080:1100	Drug release capacity of the films	1080:1113	Drug release capacity of the films was investigated in detail, and controlled release of each model drug was achieved either by tuning the polymer fraction in the films during emulsification or by crosslinking sodium alginate fraction of the films by calcium salt solution immersion.
29928979	3	43	contain	containing	475:484	arg1	emulsions					465:473	emulsions	465:473	emulsions containing dissolved biopolymers both in the oil and water phases	465:539	However, emulsions containing dissolved biopolymers both in the oil and water phases are very scarce.
29928979	3	43	contain	containing	475:484	arg2	biopolymers					496:506	dissolved biopolymers	486:506	dissolved biopolymers	486:506	However, emulsions containing dissolved biopolymers both in the oil and water phases are very scarce.
29928979	4	44	theme	hydrophobic	640:650	arg1	polymer					666:672	a hydrophobic biodegradable polymer	638:672	a hydrophobic biodegradable polymer	638:672	In this study, we demonstrate such an emulsion, in which the oil phase contains a hydrophobic biodegradable polymer, MaterBi®, and the water phase is aqueous sodium alginate dispersion.
29928979	4	44	theme	hydrophobic	640:650	arg1	MaterBi®					675:682	MaterBi®	675:682	MaterBi®	675:682	In this study, we demonstrate such an emulsion, in which the oil phase contains a hydrophobic biodegradable polymer, MaterBi®, and the water phase is aqueous sodium alginate dispersion.
29928979	0	45	theme	hydrophilic	26:36	arg1	release					64:70	in vitro hydrophilic and hydrophobic dual drug release	17:70	in vitro hydrophilic and hydrophobic dual drug release from polymeric films	17:91	Investigation of in vitro hydrophilic and hydrophobic dual drug release from polymeric films produced by sodium alginate-MaterBi® drying emulsions.
29928979	6	46	theme	several	862:868	arg1	days					870:873	several days	862:873	several days	862:873	The emulsions were stable for several days and were dried into composite solid films with varying MaterBi®/alginate fractions.
29928979	3	47	theme	dissolved	486:494	arg1	biopolymers					496:506	dissolved biopolymers	486:506	dissolved biopolymers	486:506	However, emulsions containing dissolved biopolymers both in the oil and water phases are very scarce.
29928979	0	48	theme	dual	54:57	arg1	release					64:70	in vitro hydrophilic and hydrophobic dual drug release	17:70	in vitro hydrophilic and hydrophobic dual drug release from polymeric films	17:91	Investigation of in vitro hydrophilic and hydrophobic dual drug release from polymeric films produced by sodium alginate-MaterBi® drying emulsions.
29928979	10	49	theme	human	1542:1546	arg1	cells					1573:1577	human dermal fibroblast, adult cells	1542:1577	cells	1573:1577	Films demonstrate excellent cell biocompatibility against human dermal fibroblast, adult cells.
29928979	4	50	contain	contains	629:636	arg1	emulsion					596:603	such an emulsion	588:603	such an emulsion	588:603	In this study, we demonstrate such an emulsion, in which the oil phase contains a hydrophobic biodegradable polymer, MaterBi®, and the water phase is aqueous sodium alginate dispersion.
29928979	4	50	contain	contains	629:636	arg2	phase					623:627	the oil phase	615:627	the oil phase	615:627	In this study, we demonstrate such an emulsion, in which the oil phase contains a hydrophobic biodegradable polymer, MaterBi®, and the water phase is aqueous sodium alginate dispersion.
29928979	4	50	contain	contains	629:636	arg1	phase					623:627	the oil phase	615:627	the oil phase	615:627	In this study, we demonstrate such an emulsion, in which the oil phase contains a hydrophobic biodegradable polymer, MaterBi®, and the water phase is aqueous sodium alginate dispersion.
29928979	4	50	contain	contains	629:636	arg2	polymer					666:672	a hydrophobic biodegradable polymer	638:672	a hydrophobic biodegradable polymer	638:672	In this study, we demonstrate such an emulsion, in which the oil phase contains a hydrophobic biodegradable polymer, MaterBi®, and the water phase is aqueous sodium alginate dispersion.
29928979	4	50	contain	contains	629:636	arg2	MaterBi®					675:682	MaterBi®	675:682	MaterBi®	675:682	In this study, we demonstrate such an emulsion, in which the oil phase contains a hydrophobic biodegradable polymer, MaterBi®, and the water phase is aqueous sodium alginate dispersion.
29928979	9	51	theme	desired	1463:1469	arg1	application					1471:1481	the desired application	1459:1481	the desired application	1459:1481	The emulsions can be formulated to carry either a single model drug or both drugs depending on the desired application.
29928979	3	52	theme	oil	520:522	arg1	phases					534:539	the oil and water phases	516:539	the oil and water phases	516:539	However, emulsions containing dissolved biopolymers both in the oil and water phases are very scarce.
29928979	0	53	theme	hydrophobic	42:52	arg1	release					64:70	in vitro hydrophilic and hydrophobic dual drug release	17:70	in vitro hydrophilic and hydrophobic dual drug release from polymeric films	17:91	Investigation of in vitro hydrophilic and hydrophobic dual drug release from polymeric films produced by sodium alginate-MaterBi® drying emulsions.
29928979	7	54	theme	hydrophobic	1058:1068	arg1	drugs					996:1000	two model drugs	986:1000	two model drugs	986:1000	The films were loaded with two model drugs, a hydrophilic eosin-based cutaneous antiseptic and the hydrophobic curcumin.
29928979	7	54	theme	hydrophobic	1058:1068	arg1	curcumin					1070:1077	the hydrophobic curcumin	1054:1077	the hydrophobic curcumin	1054:1077	The films were loaded with two model drugs, a hydrophilic eosin-based cutaneous antiseptic and the hydrophobic curcumin.
29928979	2	55	theme	phase	349:353	arg1	emulsions					355:363	high internal phase emulsions	335:363	high internal phase emulsions	335:363	Recently, high internal phase emulsions have also been used to produce porous polymeric templates for pharmaceutical applications.
29928979	4	56	theme	sodium	716:721	arg1	dispersion					732:741	aqueous sodium alginate dispersion	708:741	aqueous sodium alginate dispersion	708:741	In this study, we demonstrate such an emulsion, in which the oil phase contains a hydrophobic biodegradable polymer, MaterBi®, and the water phase is aqueous sodium alginate dispersion.
29928979	4	56	theme	sodium	716:721	arg1	phase					699:703	the water phase	689:703	the water phase	689:703	In this study, we demonstrate such an emulsion, in which the oil phase contains a hydrophobic biodegradable polymer, MaterBi®, and the water phase is aqueous sodium alginate dispersion.
29928979	6	57	theme	MaterBi®/alginate	930:946	arg1	fractions					948:956	varying MaterBi®/alginate fractions	922:956	varying MaterBi®/alginate fractions	922:956	The emulsions were stable for several days and were dried into composite solid films with varying MaterBi®/alginate fractions.
29928979	8	58	dep	achieved	1189:1196	arg1	tuning					1208:1213	tuning	1208:1213	tuning	1208:1213	Drug release capacity of the films was investigated in detail, and controlled release of each model drug was achieved either by tuning the polymer fraction in the films during emulsification or by crosslinking sodium alginate fraction of the films by calcium salt solution immersion.
29928979	8	58	dep	achieved	1189:1196	arg1	crosslinking					1277:1288	crosslinking	1277:1288	crosslinking sodium alginate fraction of the films by calcium salt solution immersion	1277:1361	Drug release capacity of the films was investigated in detail, and controlled release of each model drug was achieved either by tuning the polymer fraction in the films during emulsification or by crosslinking sodium alginate fraction of the films by calcium salt solution immersion.
29928979	9	59	contain	carry	1399:1403	arg2	drug					1427:1430	a single model drug	1412:1430	a single model drug	1412:1430	The emulsions can be formulated to carry either a single model drug or both drugs depending on the desired application.
29928979	9	59	contain	carry	1399:1403	arg1	emulsions					1368:1376	The emulsions	1364:1376	The emulsions	1364:1376	The emulsions can be formulated to carry either a single model drug or both drugs depending on the desired application.
29928979	9	59	contain	carry	1399:1403	arg2	drugs					1440:1444	both drugs	1435:1444	both drugs	1435:1444	The emulsions can be formulated to carry either a single model drug or both drugs depending on the desired application.
29928979	2	60	theme	internal	340:347	arg1	emulsions					355:363	high internal phase emulsions	335:363	high internal phase emulsions	335:363	Recently, high internal phase emulsions have also been used to produce porous polymeric templates for pharmaceutical applications.
29928979	10	61	theme	cell	1512:1515	arg1	biocompatibility					1517:1532	excellent cell biocompatibility	1502:1532	excellent cell biocompatibility against human dermal fibroblast, adult cells	1502:1577	Films demonstrate excellent cell biocompatibility against human dermal fibroblast, adult cells.
29928979	0	62	dep	in	17:18	arg1	vitro					20:24	vitro	20:24	vitro	20:24	Investigation of in vitro hydrophilic and hydrophobic dual drug release from polymeric films produced by sodium alginate-MaterBi® drying emulsions.
29928979	2	63	theme	high	335:338	arg1	emulsions					355:363	high internal phase emulsions	335:363	high internal phase emulsions	335:363	Recently, high internal phase emulsions have also been used to produce porous polymeric templates for pharmaceutical applications.
29928979	8	64	theme	calcium	1331:1337	arg1	solution					1344:1351	calcium salt solution	1331:1351	calcium salt solution immersion	1331:1361	Drug release capacity of the films was investigated in detail, and controlled release of each model drug was achieved either by tuning the polymer fraction in the films during emulsification or by crosslinking sodium alginate fraction of the films by calcium salt solution immersion.
29928979	1	65	theme	injectable	232:241	arg1	emulsions					243:251	particularly injectable emulsions	219:251	particularly injectable emulsions	219:251	Emulsions are known to be effective carriers of hydrophobic drugs, and particularly injectable emulsions have been successfully implemented for in vivo controlled drug release.
29928979	0	66	theme	release	64:70	arg1	Investigation					0:12	Investigation	0:12	Investigation of in vitro hydrophilic and hydrophobic dual drug release from polymeric films	0:91	Investigation of in vitro hydrophilic and hydrophobic dual drug release from polymeric films produced by sodium alginate-MaterBi® drying emulsions.
29928979	0	67	from	films	87:91	arg1	Investigation					0:12	Investigation	0:12	Investigation of in vitro hydrophilic and hydrophobic dual drug release from polymeric films	0:91	Investigation of in vitro hydrophilic and hydrophobic dual drug release from polymeric films produced by sodium alginate-MaterBi® drying emulsions.
29928979	0	67	from	films	87:91	arg1	release					64:70	in vitro hydrophilic and hydrophobic dual drug release	17:70	in vitro hydrophilic and hydrophobic dual drug release from polymeric films	17:91	Investigation of in vitro hydrophilic and hydrophobic dual drug release from polymeric films produced by sodium alginate-MaterBi® drying emulsions.
29928979	8	68	theme	films	1322:1326	arg1	fraction					1306:1313	sodium alginate fraction	1290:1313	sodium alginate fraction of the films	1290:1326	Drug release capacity of the films was investigated in detail, and controlled release of each model drug was achieved either by tuning the polymer fraction in the films during emulsification or by crosslinking sodium alginate fraction of the films by calcium salt solution immersion.
29928979	2	69	theme	porous	396:401	arg1	templates					413:421	porous polymeric templates	396:421	porous polymeric templates for pharmaceutical applications	396:453	Recently, high internal phase emulsions have also been used to produce porous polymeric templates for pharmaceutical applications.
29928979	4	70	theme	oil	619:621	arg1	phase					623:627	the oil phase	615:627	the oil phase	615:627	In this study, we demonstrate such an emulsion, in which the oil phase contains a hydrophobic biodegradable polymer, MaterBi®, and the water phase is aqueous sodium alginate dispersion.
29928979	0	71	theme	drug	59:62	arg1	release					64:70	in vitro hydrophilic and hydrophobic dual drug release	17:70	in vitro hydrophilic and hydrophobic dual drug release from polymeric films	17:91	Investigation of in vitro hydrophilic and hydrophobic dual drug release from polymeric films produced by sodium alginate-MaterBi® drying emulsions.
29928979	6	72	theme	varying	922:928	arg1	fractions					948:956	varying MaterBi®/alginate fractions	922:956	varying MaterBi®/alginate fractions	922:956	The emulsions were stable for several days and were dried into composite solid films with varying MaterBi®/alginate fractions.
29928979	8	73	theme	sodium	1290:1295	arg1	fraction					1306:1313	sodium alginate fraction	1290:1313	sodium alginate fraction of the films	1290:1326	Drug release capacity of the films was investigated in detail, and controlled release of each model drug was achieved either by tuning the polymer fraction in the films during emulsification or by crosslinking sodium alginate fraction of the films by calcium salt solution immersion.
30392956	3	0	theme	cytosolic	309:317	arg1	LTA					319:321	cytosolic LTA	309:321	cytosolic LTA	309:321	In response to cytosolic LTA or infection with Listeria monocytogenes, NLRP6 recruited caspase-11 and caspase-1 via the adaptor ASC.
30392956	4	1	theme	caspase-1	500:508	arg1	activation					510:519	caspase-1 activation	500:519	caspase-1 activation	500:519	NLRP6 activation by LTA induced processing of caspase-11, which promoted caspase-1 activation and interleukin-1β (IL-1β)/IL-18 maturation in macrophages.
30392956	4	2	theme	interleukin-1β	525:538	arg1	maturation					554:563	interleukin-1β (IL-1β)/IL-18 maturation	525:563	interleukin-1β (IL-1β)/IL-18 maturation	525:563	NLRP6 activation by LTA induced processing of caspase-11, which promoted caspase-1 activation and interleukin-1β (IL-1β)/IL-18 maturation in macrophages.
30392956	7	3	theme	Gram-positive	1017:1029	arg1	infection					1040:1048	systemic Gram-positive pathogen infection	1008:1048	systemic Gram-positive pathogen infection	1008:1048	These results reveal a previously unrecognized innate immunity pathway triggered by cytosolic LTA that is sensed by NLRP6 and exacerbates systemic Gram-positive pathogen infection via the production of IL-18.
30392956	1	4	dep	activator	104:112	arg1	The					100:102	The	100:102	The	100:102	The activator and composition of the NLRP6 inflammasome remain poorly understood.
30392956	7	5	theme	immunity	924:931	arg1	pathway					933:939	a previously unrecognized innate immunity pathway	891:939	a previously unrecognized innate immunity pathway triggered by cytosolic LTA that is sensed by NLRP6 and exacerbates systemic Gram-positive pathogen infection via the production of IL-18	891:1076	These results reveal a previously unrecognized innate immunity pathway triggered by cytosolic LTA that is sensed by NLRP6 and exacerbates systemic Gram-positive pathogen infection via the production of IL-18.
30392956	6	6	theme	L. monocytogenes	842:857	arg1	infection					859:867	L. monocytogenes infection	842:867	L. monocytogenes infection	842:867	Administration of IL-18 to Nlrp6-/- or Casp11-/- mice restored the susceptibility of mutant mice to L. monocytogenes infection.
30392956	4	7	dep	induced	451:457	arg1	promoted					491:498	promoted	491:498	promoted caspase-1 activation and interleukin-1β (IL-1β)/IL-18 maturation in macrophages	491:578	NLRP6 activation by LTA induced processing of caspase-11, which promoted caspase-1 activation and interleukin-1β (IL-1β)/IL-18 maturation in macrophages.
30392956	5	8	theme	reduced	688:694	arg1	loads					705:709	reduced pathogen loads	688:709	reduced pathogen loads	688:709	Nlrp6-/- and Casp11-/- mice were less susceptible to L. monocytogenes infection, which was associated with reduced pathogen loads and impaired IL-18 production.
30392956	7	9	theme	unrecognized	904:915	arg1	pathway					933:939	a previously unrecognized innate immunity pathway	891:939	a previously unrecognized innate immunity pathway triggered by cytosolic LTA that is sensed by NLRP6 and exacerbates systemic Gram-positive pathogen infection via the production of IL-18	891:1076	These results reveal a previously unrecognized innate immunity pathway triggered by cytosolic LTA that is sensed by NLRP6 and exacerbates systemic Gram-positive pathogen infection via the production of IL-18.
30392956	4	10	theme	caspase-11	473:482	arg1	processing					459:468	processing	459:468	processing of caspase-11	459:482	NLRP6 activation by LTA induced processing of caspase-11, which promoted caspase-1 activation and interleukin-1β (IL-1β)/IL-18 maturation in macrophages.
30392956	5	11	theme	L. monocytogenes	634:649	arg1	infection					651:659	L. monocytogenes infection	634:659	L. monocytogenes infection	634:659	Nlrp6-/- and Casp11-/- mice were less susceptible to L. monocytogenes infection, which was associated with reduced pathogen loads and impaired IL-18 production.
30392956	6	12	theme	mice	834:837	arg1	susceptibility					809:822	the susceptibility	805:822	the susceptibility of mutant mice to L. monocytogenes infection	805:867	Administration of IL-18 to Nlrp6-/- or Casp11-/- mice restored the susceptibility of mutant mice to L. monocytogenes infection.
30392956	7	13	theme	innate	917:922	arg1	pathway					933:939	a previously unrecognized innate immunity pathway	891:939	a previously unrecognized innate immunity pathway triggered by cytosolic LTA that is sensed by NLRP6 and exacerbates systemic Gram-positive pathogen infection via the production of IL-18	891:1076	These results reveal a previously unrecognized innate immunity pathway triggered by cytosolic LTA that is sensed by NLRP6 and exacerbates systemic Gram-positive pathogen infection via the production of IL-18.
30392956	3	14	theme	Listeria	341:348	arg1	monocytogenes					350:362	Listeria monocytogenes	341:362	Listeria monocytogenes	341:362	In response to cytosolic LTA or infection with Listeria monocytogenes, NLRP6 recruited caspase-11 and caspase-1 via the adaptor ASC.
30392956	5	15	theme	Nlrp6-/-	581:588	arg1	mice					604:607	Nlrp6-/- and Casp11-/- mice	581:607	Nlrp6-/- and Casp11-/- mice	581:607	Nlrp6-/- and Casp11-/- mice were less susceptible to L. monocytogenes infection, which was associated with reduced pathogen loads and impaired IL-18 production.
30392956	0	16	theme	NLRP6	4:8	arg1	Inflammasome					10:21	The NLRP6 Inflammasome	0:21	The NLRP6 Inflammasome	0:21	The NLRP6 Inflammasome Recognizes Lipoteichoic Acid and Regulates Gram-Positive Pathogen Infection.
30392956	5	17	theme	Casp11-/-	594:602	arg1	mice					604:607	Nlrp6-/- and Casp11-/- mice	581:607	Nlrp6-/- and Casp11-/- mice	581:607	Nlrp6-/- and Casp11-/- mice were less susceptible to L. monocytogenes infection, which was associated with reduced pathogen loads and impaired IL-18 production.
30392956	5	18	theme	IL-18	724:728	arg1	production					730:739	impaired IL-18 production	715:739	impaired IL-18 production	715:739	Nlrp6-/- and Casp11-/- mice were less susceptible to L. monocytogenes infection, which was associated with reduced pathogen loads and impaired IL-18 production.
30392956	7	19	theme	systemic	1008:1015	arg1	infection					1040:1048	systemic Gram-positive pathogen infection	1008:1048	systemic Gram-positive pathogen infection	1008:1048	These results reveal a previously unrecognized innate immunity pathway triggered by cytosolic LTA that is sensed by NLRP6 and exacerbates systemic Gram-positive pathogen infection via the production of IL-18.
30392956	3	20	dep	LTA	319:321	arg1	response					297:304	response	297:304	response	297:304	In response to cytosolic LTA or infection with Listeria monocytogenes, NLRP6 recruited caspase-11 and caspase-1 via the adaptor ASC.
30392956	2	21	theme	lipoteichoic	195:206	arg1	molecule					222:229	a molecule	220:229	a molecule produced by Gram-positive bacteria	220:264	We find that lipoteichoic acid (LTA), a molecule produced by Gram-positive bacteria, binds and activates NLRP6.
30392956	2	21	theme	lipoteichoic	195:206	arg1	LTA					214:216	LTA	214:216	LTA	214:216	We find that lipoteichoic acid (LTA), a molecule produced by Gram-positive bacteria, binds and activates NLRP6.
30392956	2	21	theme	lipoteichoic	195:206	arg1	acid					208:211	lipoteichoic acid	195:211	lipoteichoic acid (LTA)	195:217	We find that lipoteichoic acid (LTA), a molecule produced by Gram-positive bacteria, binds and activates NLRP6.
30392956	0	22	theme	Lipoteichoic	34:45	arg1	Acid					47:50	Lipoteichoic Acid	34:50	Lipoteichoic Acid	34:50	The NLRP6 Inflammasome Recognizes Lipoteichoic Acid and Regulates Gram-Positive Pathogen Infection.
30392956	3	23	theme	adaptor	414:420	arg1	ASC					422:424	the adaptor ASC	410:424	the adaptor ASC	410:424	In response to cytosolic LTA or infection with Listeria monocytogenes, NLRP6 recruited caspase-11 and caspase-1 via the adaptor ASC.
30392956	5	24	theme	pathogen	696:703	arg1	loads					705:709	reduced pathogen loads	688:709	reduced pathogen loads	688:709	Nlrp6-/- and Casp11-/- mice were less susceptible to L. monocytogenes infection, which was associated with reduced pathogen loads and impaired IL-18 production.
30392956	4	25	theme	/IL-18	547:552	arg1	maturation					554:563	interleukin-1β (IL-1β)/IL-18 maturation	525:563	interleukin-1β (IL-1β)/IL-18 maturation	525:563	NLRP6 activation by LTA induced processing of caspase-11, which promoted caspase-1 activation and interleukin-1β (IL-1β)/IL-18 maturation in macrophages.
30392956	7	26	theme	IL-18	1072:1076	arg1	production					1058:1067	the production	1054:1067	the production of IL-18	1054:1076	These results reveal a previously unrecognized innate immunity pathway triggered by cytosolic LTA that is sensed by NLRP6 and exacerbates systemic Gram-positive pathogen infection via the production of IL-18.
30392956	6	27	theme	Casp11-/-	781:789	arg1	mice					791:794	Nlrp6-/- or Casp11-/- mice	769:794	Nlrp6-/- or Casp11-/- mice	769:794	Administration of IL-18 to Nlrp6-/- or Casp11-/- mice restored the susceptibility of mutant mice to L. monocytogenes infection.
30392956	6	28	theme	mutant	827:832	arg1	mice					834:837	mutant mice	827:837	mutant mice	827:837	Administration of IL-18 to Nlrp6-/- or Casp11-/- mice restored the susceptibility of mutant mice to L. monocytogenes infection.
30392956	7	29	theme	pathogen	1031:1038	arg1	infection					1040:1048	systemic Gram-positive pathogen infection	1008:1048	systemic Gram-positive pathogen infection	1008:1048	These results reveal a previously unrecognized innate immunity pathway triggered by cytosolic LTA that is sensed by NLRP6 and exacerbates systemic Gram-positive pathogen infection via the production of IL-18.
30392956	1	30	theme	NLRP6	137:141	arg1	inflammasome					143:154	the NLRP6 inflammasome	133:154	the NLRP6 inflammasome	133:154	The activator and composition of the NLRP6 inflammasome remain poorly understood.
30392956	5	31	theme	impaired	715:722	arg1	production					730:739	impaired IL-18 production	715:739	impaired IL-18 production	715:739	Nlrp6-/- and Casp11-/- mice were less susceptible to L. monocytogenes infection, which was associated with reduced pathogen loads and impaired IL-18 production.
30392956	4	32	theme	activation	433:442	arg1	by LTA					444:449	NLRP6 activation by LTA	427:449	NLRP6 activation by LTA	427:449	NLRP6 activation by LTA induced processing of caspase-11, which promoted caspase-1 activation and interleukin-1β (IL-1β)/IL-18 maturation in macrophages.
30392956	3	33	with	LTA	319:321	arg1	monocytogenes					350:362	Listeria monocytogenes	341:362	Listeria monocytogenes	341:362	In response to cytosolic LTA or infection with Listeria monocytogenes, NLRP6 recruited caspase-11 and caspase-1 via the adaptor ASC.
30392956	6	34	theme	Nlrp6-/-	769:776	arg1	mice					791:794	Nlrp6-/- or Casp11-/- mice	769:794	Nlrp6-/- or Casp11-/- mice	769:794	Administration of IL-18 to Nlrp6-/- or Casp11-/- mice restored the susceptibility of mutant mice to L. monocytogenes infection.
30392956	1	35	theme	inflammasome	143:154	arg1	activator					104:112	activator	104:112	activator	104:112	The activator and composition of the NLRP6 inflammasome remain poorly understood.
30392956	1	35	theme	inflammasome	143:154	arg1	composition					118:128	composition	118:128	composition	118:128	The activator and composition of the NLRP6 inflammasome remain poorly understood.
30392956	7	36	theme	cytosolic	954:962	arg1	LTA					964:966	cytosolic LTA	954:966	cytosolic LTA	954:966	These results reveal a previously unrecognized innate immunity pathway triggered by cytosolic LTA that is sensed by NLRP6 and exacerbates systemic Gram-positive pathogen infection via the production of IL-18.
30392956	0	37	theme	Pathogen	80:87	arg1	Infection					89:97	Gram-Positive Pathogen Infection	66:97	Gram-Positive Pathogen Infection	66:97	The NLRP6 Inflammasome Recognizes Lipoteichoic Acid and Regulates Gram-Positive Pathogen Infection.
30392956	4	38	theme	NLRP6	427:431	arg1	by LTA					444:449	NLRP6 activation by LTA	427:449	NLRP6 activation by LTA	427:449	NLRP6 activation by LTA induced processing of caspase-11, which promoted caspase-1 activation and interleukin-1β (IL-1β)/IL-18 maturation in macrophages.
30392956	2	39	theme	Gram-positive	243:255	arg1	bacteria					257:264	Gram-positive bacteria	243:264	Gram-positive bacteria	243:264	We find that lipoteichoic acid (LTA), a molecule produced by Gram-positive bacteria, binds and activates NLRP6.
30392956	0	40	theme	Gram-Positive	66:78	arg1	Infection					89:97	Gram-Positive Pathogen Infection	66:97	Gram-Positive Pathogen Infection	66:97	The NLRP6 Inflammasome Recognizes Lipoteichoic Acid and Regulates Gram-Positive Pathogen Infection.
30392956	3	41	with	infection	326:334	arg1	monocytogenes					350:362	Listeria monocytogenes	341:362	Listeria monocytogenes	341:362	In response to cytosolic LTA or infection with Listeria monocytogenes, NLRP6 recruited caspase-11 and caspase-1 via the adaptor ASC.
30392956	6	42	theme	IL-18	760:764	arg1	Administration					742:755	Administration	742:755	Administration of IL-18 to Nlrp6-/- or Casp11-/- mice	742:794	Administration of IL-18 to Nlrp6-/- or Casp11-/- mice restored the susceptibility of mutant mice to L. monocytogenes infection.
31892546	12	0	theme	bacterial	1441:1449	arg1	heparinase					1451:1460	bacterial heparinase	1441:1460	bacterial heparinase	1441:1460	Higher proliferation was observed in tumour cell lines incubated with exudate than with transudate and it was reduced when bacterial heparinase was added.
31892546	2	1	theme	Human	254:258	arg1	heparanase					260:269	Human heparanase	254:269	Human heparanase	254:269	Human heparanase, involved in angiogenesis and metastasis, cleaves heparan sulfate (HS) side chains on the cell surface.
31892546	1	2	theme	significant	159:169	arg1	cause					171:175	a common and significant cause	146:175	a common and significant cause of morbidity in patients with cancer	146:212	BACKGROUND While malignant pleural effusion (MPE) is a common and significant cause of morbidity in patients with cancer, current treatment options are limited.
31892546	1	2	theme	significant	159:169	arg1	BACKGROUND					93:102	BACKGROUND	93:102	BACKGROUND While malignant pleural effusion (MPE)	93:141	BACKGROUND While malignant pleural effusion (MPE) is a common and significant cause of morbidity in patients with cancer, current treatment options are limited.
31892546	6	3	theme	breast	834:839	arg1	cells					848:852	EMT-6 breast cancer cells	828:852	EMT-6 breast cancer cells	828:852	EMT-6 breast cancer cells were injected to the pleural cavity of mice.
31892546	12	4	theme	cell	1362:1365	arg1	lines					1367:1371	tumour cell lines	1355:1371	tumour cell lines incubated with exudate	1355:1394	Higher proliferation was observed in tumour cell lines incubated with exudate than with transudate and it was reduced when bacterial heparinase was added.
31892546	14	5	from	milieu	1684:1689	arg1	MPE					1694:1696	MPE	1694:1696	MPE	1694:1696	CONCLUSIONS HS chains released by heparanase form an anticoagulant milieu in MPE, preventing local thrombosis and enabling tumour cell proliferation.
31892546	5	6	theme	cell	788:791	arg1	assay					807:811	A cell proliferation assay	786:811	A cell proliferation assay	786:811	A cell proliferation assay was performed.
31892546	1	7	from	patients	193:200	arg1	cause					171:175	a common and significant cause	146:175	a common and significant cause of morbidity in patients with cancer	146:212	BACKGROUND While malignant pleural effusion (MPE) is a common and significant cause of morbidity in patients with cancer, current treatment options are limited.
31892546	1	7	from	patients	193:200	arg1	BACKGROUND					93:102	BACKGROUND	93:102	BACKGROUND While malignant pleural effusion (MPE)	93:141	BACKGROUND While malignant pleural effusion (MPE) is a common and significant cause of morbidity in patients with cancer, current treatment options are limited.
31892546	6	8	theme	mice	893:896	arg1	cavity					883:888	the pleural cavity	871:888	the pleural cavity of mice	871:896	EMT-6 breast cancer cells were injected to the pleural cavity of mice.
31892546	14	9	theme	anticoagulant	1670:1682	arg1	milieu					1684:1689	an anticoagulant milieu	1667:1689	an anticoagulant milieu in MPE	1667:1696	CONCLUSIONS HS chains released by heparanase form an anticoagulant milieu in MPE, preventing local thrombosis and enabling tumour cell proliferation.
31892546	13	10	theme	heparanase	1536:1545	arg1	inhibitor					1547:1555	the heparanase inhibitor	1532:1555	the heparanase inhibitor	1532:1555	The tumour size in the pleural cavity of mice treated with the heparanase inhibitor were significantly smaller compared with control (p=0.005).
31892546	1	11	from	morbidity	180:188	arg1	patients					193:200	patients	193:200	patients with cancer	193:212	BACKGROUND While malignant pleural effusion (MPE) is a common and significant cause of morbidity in patients with cancer, current treatment options are limited.
31892546	4	12	theme	heparanase	661:670	arg1	ELISA					672:676	heparanase ELISA	661:676	heparanase ELISA	661:676	METHODS Samples of 30 patients with MPE and 44 patients with IPE were evaluated in comparison to those of 33 patients with transudate pleural effusions, using heparanase ELISA, heparanase procoagulant activity assay, thrombin and factor Xa chromogenic assays and thromboelastography.
31892546	1	13	from	cause	171:175	arg1	patients					193:200	patients	193:200	patients with cancer	193:212	BACKGROUND While malignant pleural effusion (MPE) is a common and significant cause of morbidity in patients with cancer, current treatment options are limited.
31892546	12	14	theme	Higher	1318:1323	arg1	proliferation					1325:1337	Higher proliferation	1318:1337	Higher proliferation	1318:1337	Higher proliferation was observed in tumour cell lines incubated with exudate than with transudate and it was reduced when bacterial heparinase was added.
31892546	13	15	theme	mice	1514:1517	arg1	cavity					1504:1509	the pleural cavity	1492:1509	the pleural cavity of mice treated with the heparanase inhibitor	1492:1555	The tumour size in the pleural cavity of mice treated with the heparanase inhibitor were significantly smaller compared with control (p=0.005).
31892546	11	16	theme	high	1271:1274	arg1	levels					1276:1281	high levels	1271:1281	high levels of HS chains in pleural effusions	1271:1315	Direct measurement revealed high levels of HS chains in pleural effusions.
31892546	13	17	from	size	1484:1487	arg1	cavity					1504:1509	the pleural cavity	1492:1509	the pleural cavity of mice treated with the heparanase inhibitor	1492:1555	The tumour size in the pleural cavity of mice treated with the heparanase inhibitor were significantly smaller compared with control (p=0.005).
31892546	4	18	theme	pleural	636:642	arg1	effusions					644:652	transudate pleural effusions	625:652	transudate pleural effusions	625:652	METHODS Samples of 30 patients with MPE and 44 patients with IPE were evaluated in comparison to those of 33 patients with transudate pleural effusions, using heparanase ELISA, heparanase procoagulant activity assay, thrombin and factor Xa chromogenic assays and thromboelastography.
31892546	0	19	theme	pleural	75:81	arg1	effusion					83:90	malignant pleural effusion	65:90	malignant pleural effusion	65:90	Heparan sulfate chains contribute to the anticoagulant milieu in malignant pleural effusion.
31892546	8	20	theme	heparanase	991:1000	arg1	Levels					981:986	RESULTS Levels	973:986	RESULTS Levels of heparanase, factor Xa and thrombin	973:1024	RESULTS Levels of heparanase, factor Xa and thrombin were significantly higher in exudate than transudate.
31892546	7	21	theme	inhibiting	909:918	arg1	activity					931:938	A peptide inhibiting heparanase activity	899:938	A peptide inhibiting heparanase activity	899:938	A peptide inhibiting heparanase activity was administered subcutaneously.
31892546	1	22	theme	common	148:153	arg1	cause					171:175	a common and significant cause	146:175	a common and significant cause of morbidity in patients with cancer	146:212	BACKGROUND While malignant pleural effusion (MPE) is a common and significant cause of morbidity in patients with cancer, current treatment options are limited.
31892546	1	22	theme	common	148:153	arg1	BACKGROUND					93:102	BACKGROUND	93:102	BACKGROUND While malignant pleural effusion (MPE)	93:141	BACKGROUND While malignant pleural effusion (MPE) is a common and significant cause of morbidity in patients with cancer, current treatment options are limited.
31892546	4	23	theme	assay	712:716	arg1	assays					754:759	heparanase ELISA, heparanase procoagulant activity assay, thrombin and factor Xa chromogenic assays	661:759	heparanase ELISA, heparanase procoagulant activity assay, thrombin and factor Xa chromogenic assays	661:759	METHODS Samples of 30 patients with MPE and 44 patients with IPE were evaluated in comparison to those of 33 patients with transudate pleural effusions, using heparanase ELISA, heparanase procoagulant activity assay, thrombin and factor Xa chromogenic assays and thromboelastography.
31892546	10	24	theme	bacterial	1221:1229	arg1	heparinase					1231:1240	bacterial heparinase	1221:1240	bacterial heparinase	1221:1240	This effect was completely reversed by bacterial heparinase.
31892546	14	25	theme	CONCLUSIONS	1617:1627	arg1	chains					1632:1637	CONCLUSIONS HS chains	1617:1637	CONCLUSIONS HS chains released by heparanase	1617:1660	CONCLUSIONS HS chains released by heparanase form an anticoagulant milieu in MPE, preventing local thrombosis and enabling tumour cell proliferation.
31892546	8	26	theme	factor	1003:1008	arg1	heparanase					991:1000	heparanase	991:1000	heparanase	991:1000	RESULTS Levels of heparanase, factor Xa and thrombin were significantly higher in exudate than transudate.
31892546	8	26	theme	factor	1003:1008	arg1	Xa					1010:1011	factor Xa	1003:1011	factor Xa	1003:1011	RESULTS Levels of heparanase, factor Xa and thrombin were significantly higher in exudate than transudate.
31892546	11	27	theme	chains	1289:1294	arg1	levels					1276:1281	high levels	1271:1281	high levels of HS chains in pleural effusions	1271:1315	Direct measurement revealed high levels of HS chains in pleural effusions.
31892546	4	28	theme	thrombin	719:726	arg1	assays					754:759	heparanase ELISA, heparanase procoagulant activity assay, thrombin and factor Xa chromogenic assays	661:759	heparanase ELISA, heparanase procoagulant activity assay, thrombin and factor Xa chromogenic assays	661:759	METHODS Samples of 30 patients with MPE and 44 patients with IPE were evaluated in comparison to those of 33 patients with transudate pleural effusions, using heparanase ELISA, heparanase procoagulant activity assay, thrombin and factor Xa chromogenic assays and thromboelastography.
31892546	15	29	theme	therapeutic	1808:1818	arg1	option					1820:1825	a therapeutic option	1806:1825	a therapeutic option for patients with recurrent MPE	1806:1857	Inhibition of heparanase might provide a therapeutic option for patients with recurrent MPE.
31892546	2	30	theme	cell	361:364	arg1	surface					366:372	the cell surface	357:372	the cell surface	357:372	Human heparanase, involved in angiogenesis and metastasis, cleaves heparan sulfate (HS) side chains on the cell surface.
31892546	0	31	theme	Heparan	0:6	arg1	chains					16:21	Heparan sulfate chains	0:21	Heparan sulfate chains	0:21	Heparan sulfate chains contribute to the anticoagulant milieu in malignant pleural effusion.
31892546	4	32	theme	METHODS	502:508	arg1	Samples					510:516	METHODS Samples	502:516	METHODS Samples of 30 patients with MPE and 44 patients with IPE	502:565	METHODS Samples of 30 patients with MPE and 44 patients with IPE were evaluated in comparison to those of 33 patients with transudate pleural effusions, using heparanase ELISA, heparanase procoagulant activity assay, thrombin and factor Xa chromogenic assays and thromboelastography.
31892546	4	33	theme	procoagulant	690:701	arg1	assay					712:716	heparanase procoagulant activity assay	679:716	heparanase procoagulant activity assay	679:716	METHODS Samples of 30 patients with MPE and 44 patients with IPE were evaluated in comparison to those of 33 patients with transudate pleural effusions, using heparanase ELISA, heparanase procoagulant activity assay, thrombin and factor Xa chromogenic assays and thromboelastography.
31892546	15	34	theme	heparanase	1781:1790	arg1	Inhibition					1767:1776	Inhibition	1767:1776	Inhibition of heparanase	1767:1790	Inhibition of heparanase might provide a therapeutic option for patients with recurrent MPE.
31892546	6	35	theme	EMT-6	828:832	arg1	cells					848:852	EMT-6 breast cancer cells	828:852	EMT-6 breast cancer cells	828:852	EMT-6 breast cancer cells were injected to the pleural cavity of mice.
31892546	1	36	theme	malignant	110:118	arg1	MPE					138:140	MPE	138:140	MPE	138:140	BACKGROUND While malignant pleural effusion (MPE) is a common and significant cause of morbidity in patients with cancer, current treatment options are limited.
31892546	1	36	theme	malignant	110:118	arg1	effusion					128:135	malignant pleural effusion	110:135	malignant pleural effusion (MPE)	110:141	BACKGROUND While malignant pleural effusion (MPE) is a common and significant cause of morbidity in patients with cancer, current treatment options are limited.
31892546	9	37	theme	no	1116:1117	arg1	formation					1128:1136	almost no thrombus formation	1109:1136	almost no thrombus formation	1109:1136	Thromboelastography detected almost no thrombus formation in the whole blood, mainly on MPE addition.
31892546	2	38	theme	sulfate	329:335	arg1	chains					347:352	heparan sulfate (HS) side chains	321:352	heparan sulfate (HS) side chains on the cell surface	321:372	Human heparanase, involved in angiogenesis and metastasis, cleaves heparan sulfate (HS) side chains on the cell surface.
31892546	0	39	theme	anticoagulant	41:53	arg1	milieu					55:60	the anticoagulant milieu	37:60	the anticoagulant milieu in malignant pleural effusion	37:90	Heparan sulfate chains contribute to the anticoagulant milieu in malignant pleural effusion.
31892546	4	40	with	patients	524:531	arg1	MPE					538:540	MPE	538:540	MPE	538:540	METHODS Samples of 30 patients with MPE and 44 patients with IPE were evaluated in comparison to those of 33 patients with transudate pleural effusions, using heparanase ELISA, heparanase procoagulant activity assay, thrombin and factor Xa chromogenic assays and thromboelastography.
31892546	4	40	with	patients	524:531	arg1	IPE					563:565	IPE	563:565	IPE	563:565	METHODS Samples of 30 patients with MPE and 44 patients with IPE were evaluated in comparison to those of 33 patients with transudate pleural effusions, using heparanase ELISA, heparanase procoagulant activity assay, thrombin and factor Xa chromogenic assays and thromboelastography.
31892546	4	41	theme	factor	732:737	arg1	Xa					739:740	factor Xa	732:740	factor Xa	732:740	METHODS Samples of 30 patients with MPE and 44 patients with IPE were evaluated in comparison to those of 33 patients with transudate pleural effusions, using heparanase ELISA, heparanase procoagulant activity assay, thrombin and factor Xa chromogenic assays and thromboelastography.
31892546	3	42	theme	pleural	436:442	arg1	IPE					454:456	IPE	454:456	IPE	454:456	AIMS To explore the coagulation milieu in MPE and infectious pleural effusion (IPE) focusing on the involvement of heparanase.
31892546	3	42	theme	pleural	436:442	arg1	effusion					444:451	infectious pleural effusion	425:451	infectious pleural effusion (IPE) focusing on the involvement of heparanase	425:499	AIMS To explore the coagulation milieu in MPE and infectious pleural effusion (IPE) focusing on the involvement of heparanase.
31892546	4	43	theme	chromogenic	742:752	arg1	assays					754:759	heparanase ELISA, heparanase procoagulant activity assay, thrombin and factor Xa chromogenic assays	661:759	heparanase ELISA, heparanase procoagulant activity assay, thrombin and factor Xa chromogenic assays	661:759	METHODS Samples of 30 patients with MPE and 44 patients with IPE were evaluated in comparison to those of 33 patients with transudate pleural effusions, using heparanase ELISA, heparanase procoagulant activity assay, thrombin and factor Xa chromogenic assays and thromboelastography.
31892546	2	44	theme	side	342:345	arg1	chains					347:352	heparan sulfate (HS) side chains	321:352	heparan sulfate (HS) side chains on the cell surface	321:372	Human heparanase, involved in angiogenesis and metastasis, cleaves heparan sulfate (HS) side chains on the cell surface.
31892546	14	45	theme	tumour	1740:1745	arg1	proliferation					1752:1764	tumour cell proliferation	1740:1764	tumour cell proliferation	1740:1764	CONCLUSIONS HS chains released by heparanase form an anticoagulant milieu in MPE, preventing local thrombosis and enabling tumour cell proliferation.
31892546	6	46	theme	pleural	875:881	arg1	cavity					883:888	the pleural cavity	871:888	the pleural cavity of mice	871:896	EMT-6 breast cancer cells were injected to the pleural cavity of mice.
31892546	15	47	theme	recurrent	1845:1853	arg1	MPE					1855:1857	recurrent MPE	1845:1857	recurrent MPE	1845:1857	Inhibition of heparanase might provide a therapeutic option for patients with recurrent MPE.
31892546	3	48	from	milieu	407:412	arg1	MPE					417:419	MPE	417:419	MPE	417:419	AIMS To explore the coagulation milieu in MPE and infectious pleural effusion (IPE) focusing on the involvement of heparanase.
31892546	3	48	from	milieu	407:412	arg1	IPE					454:456	IPE	454:456	IPE	454:456	AIMS To explore the coagulation milieu in MPE and infectious pleural effusion (IPE) focusing on the involvement of heparanase.
31892546	3	48	from	milieu	407:412	arg1	effusion					444:451	infectious pleural effusion	425:451	infectious pleural effusion (IPE) focusing on the involvement of heparanase	425:499	AIMS To explore the coagulation milieu in MPE and infectious pleural effusion (IPE) focusing on the involvement of heparanase.
31892546	4	49	theme	patients	524:531	arg1	Samples					510:516	METHODS Samples	502:516	METHODS Samples of 30 patients with MPE and 44 patients with IPE	502:565	METHODS Samples of 30 patients with MPE and 44 patients with IPE were evaluated in comparison to those of 33 patients with transudate pleural effusions, using heparanase ELISA, heparanase procoagulant activity assay, thrombin and factor Xa chromogenic assays and thromboelastography.
31892546	3	50	theme	heparanase	490:499	arg1	involvement					475:485	the involvement	471:485	the involvement of heparanase	471:499	AIMS To explore the coagulation milieu in MPE and infectious pleural effusion (IPE) focusing on the involvement of heparanase.
31892546	14	51	theme	local	1710:1714	arg1	thrombosis					1716:1725	local thrombosis	1710:1725	local thrombosis	1710:1725	CONCLUSIONS HS chains released by heparanase form an anticoagulant milieu in MPE, preventing local thrombosis and enabling tumour cell proliferation.
31892546	9	52	theme	MPE	1168:1170	arg1	addition					1172:1179	MPE addition	1168:1179	MPE addition	1168:1179	Thromboelastography detected almost no thrombus formation in the whole blood, mainly on MPE addition.
31892546	6	53	theme	cancer	841:846	arg1	cells					848:852	EMT-6 breast cancer cells	828:852	EMT-6 breast cancer cells	828:852	EMT-6 breast cancer cells were injected to the pleural cavity of mice.
31892546	8	54	theme	RESULTS	973:979	arg1	Levels					981:986	RESULTS Levels	973:986	RESULTS Levels of heparanase, factor Xa and thrombin	973:1024	RESULTS Levels of heparanase, factor Xa and thrombin were significantly higher in exudate than transudate.
31892546	4	55	with	patients	611:618	arg1	effusions					644:652	transudate pleural effusions	625:652	transudate pleural effusions	625:652	METHODS Samples of 30 patients with MPE and 44 patients with IPE were evaluated in comparison to those of 33 patients with transudate pleural effusions, using heparanase ELISA, heparanase procoagulant activity assay, thrombin and factor Xa chromogenic assays and thromboelastography.
31892546	12	56	theme	tumour	1355:1360	arg1	lines					1367:1371	tumour cell lines	1355:1371	tumour cell lines incubated with exudate	1355:1394	Higher proliferation was observed in tumour cell lines incubated with exudate than with transudate and it was reduced when bacterial heparinase was added.
31892546	11	57	theme	pleural	1299:1305	arg1	effusions					1307:1315	pleural effusions	1299:1315	pleural effusions	1299:1315	Direct measurement revealed high levels of HS chains in pleural effusions.
31892546	1	58	theme	morbidity	180:188	arg1	cause					171:175	a common and significant cause	146:175	a common and significant cause of morbidity in patients with cancer	146:212	BACKGROUND While malignant pleural effusion (MPE) is a common and significant cause of morbidity in patients with cancer, current treatment options are limited.
31892546	1	58	theme	morbidity	180:188	arg1	BACKGROUND					93:102	BACKGROUND	93:102	BACKGROUND While malignant pleural effusion (MPE)	93:141	BACKGROUND While malignant pleural effusion (MPE) is a common and significant cause of morbidity in patients with cancer, current treatment options are limited.
31892546	5	59	theme	proliferation	793:805	arg1	assay					807:811	A cell proliferation assay	786:811	A cell proliferation assay	786:811	A cell proliferation assay was performed.
31892546	4	60	with	patients	549:556	arg1	MPE					538:540	MPE	538:540	MPE	538:540	METHODS Samples of 30 patients with MPE and 44 patients with IPE were evaluated in comparison to those of 33 patients with transudate pleural effusions, using heparanase ELISA, heparanase procoagulant activity assay, thrombin and factor Xa chromogenic assays and thromboelastography.
31892546	4	60	with	patients	549:556	arg1	IPE					563:565	IPE	563:565	IPE	563:565	METHODS Samples of 30 patients with MPE and 44 patients with IPE were evaluated in comparison to those of 33 patients with transudate pleural effusions, using heparanase ELISA, heparanase procoagulant activity assay, thrombin and factor Xa chromogenic assays and thromboelastography.
31892546	1	61	theme	pleural	120:126	arg1	MPE					138:140	MPE	138:140	MPE	138:140	BACKGROUND While malignant pleural effusion (MPE) is a common and significant cause of morbidity in patients with cancer, current treatment options are limited.
31892546	1	61	theme	pleural	120:126	arg1	effusion					128:135	malignant pleural effusion	110:135	malignant pleural effusion (MPE)	110:141	BACKGROUND While malignant pleural effusion (MPE) is a common and significant cause of morbidity in patients with cancer, current treatment options are limited.
31892546	13	62	theme	pleural	1496:1502	arg1	cavity					1504:1509	the pleural cavity	1492:1509	the pleural cavity of mice treated with the heparanase inhibitor	1492:1555	The tumour size in the pleural cavity of mice treated with the heparanase inhibitor were significantly smaller compared with control (p=0.005).
31892546	0	63	theme	malignant	65:73	arg1	effusion					83:90	malignant pleural effusion	65:90	malignant pleural effusion	65:90	Heparan sulfate chains contribute to the anticoagulant milieu in malignant pleural effusion.
31892546	4	64	theme	transudate	625:634	arg1	effusions					644:652	transudate pleural effusions	625:652	transudate pleural effusions	625:652	METHODS Samples of 30 patients with MPE and 44 patients with IPE were evaluated in comparison to those of 33 patients with transudate pleural effusions, using heparanase ELISA, heparanase procoagulant activity assay, thrombin and factor Xa chromogenic assays and thromboelastography.
31892546	14	65	theme	HS	1629:1630	arg1	chains					1632:1637	CONCLUSIONS HS chains	1617:1637	CONCLUSIONS HS chains released by heparanase	1617:1660	CONCLUSIONS HS chains released by heparanase form an anticoagulant milieu in MPE, preventing local thrombosis and enabling tumour cell proliferation.
31892546	7	66	theme	heparanase	920:929	arg1	activity					931:938	A peptide inhibiting heparanase activity	899:938	A peptide inhibiting heparanase activity	899:938	A peptide inhibiting heparanase activity was administered subcutaneously.
31892546	0	67	from	milieu	55:60	arg1	effusion					83:90	malignant pleural effusion	65:90	malignant pleural effusion	65:90	Heparan sulfate chains contribute to the anticoagulant milieu in malignant pleural effusion.
31892546	4	68	theme	activity	703:710	arg1	assay					712:716	heparanase procoagulant activity assay	679:716	heparanase procoagulant activity assay	679:716	METHODS Samples of 30 patients with MPE and 44 patients with IPE were evaluated in comparison to those of 33 patients with transudate pleural effusions, using heparanase ELISA, heparanase procoagulant activity assay, thrombin and factor Xa chromogenic assays and thromboelastography.
31892546	7	69	theme	peptide	901:907	arg1	activity					931:938	A peptide inhibiting heparanase activity	899:938	A peptide inhibiting heparanase activity	899:938	A peptide inhibiting heparanase activity was administered subcutaneously.
31892546	0	70	theme	sulfate	8:14	arg1	chains					16:21	Heparan sulfate chains	0:21	Heparan sulfate chains	0:21	Heparan sulfate chains contribute to the anticoagulant milieu in malignant pleural effusion.
31892546	11	71	theme	HS	1286:1287	arg1	chains					1289:1294	HS chains	1286:1294	HS chains	1286:1294	Direct measurement revealed high levels of HS chains in pleural effusions.
31892546	4	72	theme	ELISA	672:676	arg1	assays					754:759	heparanase ELISA, heparanase procoagulant activity assay, thrombin and factor Xa chromogenic assays	661:759	heparanase ELISA, heparanase procoagulant activity assay, thrombin and factor Xa chromogenic assays	661:759	METHODS Samples of 30 patients with MPE and 44 patients with IPE were evaluated in comparison to those of 33 patients with transudate pleural effusions, using heparanase ELISA, heparanase procoagulant activity assay, thrombin and factor Xa chromogenic assays and thromboelastography.
31892546	12	73	located	observed	1343:1350	arg1	lines					1367:1371	tumour cell lines	1355:1371	tumour cell lines incubated with exudate	1355:1394	Higher proliferation was observed in tumour cell lines incubated with exudate than with transudate and it was reduced when bacterial heparinase was added.
31892546	12	73	located	observed	1343:1350	arg2	proliferation					1325:1337	Higher proliferation	1318:1337	Higher proliferation	1318:1337	Higher proliferation was observed in tumour cell lines incubated with exudate than with transudate and it was reduced when bacterial heparinase was added.
31892546	1	74	with	patients	193:200	arg1	cancer					207:212	cancer	207:212	cancer	207:212	BACKGROUND While malignant pleural effusion (MPE) is a common and significant cause of morbidity in patients with cancer, current treatment options are limited.
31892546	14	75	dep	form	1662:1665	arg1	preventing					1699:1708	preventing	1699:1708	preventing local thrombosis	1699:1725	CONCLUSIONS HS chains released by heparanase form an anticoagulant milieu in MPE, preventing local thrombosis and enabling tumour cell proliferation.
31892546	14	75	dep	form	1662:1665	arg1	enabling					1731:1738	enabling	1731:1738	enabling tumour cell proliferation	1731:1764	CONCLUSIONS HS chains released by heparanase form an anticoagulant milieu in MPE, preventing local thrombosis and enabling tumour cell proliferation.
31892546	4	76	theme	heparanase	679:688	arg1	assay					712:716	heparanase procoagulant activity assay	679:716	heparanase procoagulant activity assay	679:716	METHODS Samples of 30 patients with MPE and 44 patients with IPE were evaluated in comparison to those of 33 patients with transudate pleural effusions, using heparanase ELISA, heparanase procoagulant activity assay, thrombin and factor Xa chromogenic assays and thromboelastography.
31892546	3	77	theme	infectious	425:434	arg1	IPE					454:456	IPE	454:456	IPE	454:456	AIMS To explore the coagulation milieu in MPE and infectious pleural effusion (IPE) focusing on the involvement of heparanase.
31892546	3	77	theme	infectious	425:434	arg1	effusion					444:451	infectious pleural effusion	425:451	infectious pleural effusion (IPE) focusing on the involvement of heparanase	425:499	AIMS To explore the coagulation milieu in MPE and infectious pleural effusion (IPE) focusing on the involvement of heparanase.
31892546	3	78	theme	coagulation	395:405	arg1	milieu					407:412	the coagulation milieu	391:412	the coagulation milieu in MPE and infectious pleural effusion (IPE) focusing on the involvement of heparanase	391:499	AIMS To explore the coagulation milieu in MPE and infectious pleural effusion (IPE) focusing on the involvement of heparanase.
31892546	1	79	theme	current	215:221	arg1	options					233:239	current treatment options	215:239	current treatment options	215:239	BACKGROUND While malignant pleural effusion (MPE) is a common and significant cause of morbidity in patients with cancer, current treatment options are limited.
31892546	13	80	theme	tumour	1477:1482	arg1	size					1484:1487	The tumour size	1473:1487	The tumour size in the pleural cavity of mice treated with the heparanase inhibitor	1473:1555	The tumour size in the pleural cavity of mice treated with the heparanase inhibitor were significantly smaller compared with control (p=0.005).
31892546	13	80	theme	tumour	1477:1482	arg1	smaller					1576:1582	smaller	1576:1582	smaller	1576:1582	The tumour size in the pleural cavity of mice treated with the heparanase inhibitor were significantly smaller compared with control (p=0.005).
31892546	15	81	with	patients	1831:1838	arg1	MPE					1855:1857	recurrent MPE	1845:1857	recurrent MPE	1845:1857	Inhibition of heparanase might provide a therapeutic option for patients with recurrent MPE.
31892546	2	82	from	chains	347:352	arg1	surface					366:372	the cell surface	357:372	the cell surface	357:372	Human heparanase, involved in angiogenesis and metastasis, cleaves heparan sulfate (HS) side chains on the cell surface.
31892546	11	83	theme	Direct	1243:1248	arg1	measurement					1250:1260	Direct measurement	1243:1260	Direct measurement	1243:1260	Direct measurement revealed high levels of HS chains in pleural effusions.
31892546	9	84	theme	thrombus	1119:1126	arg1	formation					1128:1136	almost no thrombus formation	1109:1136	almost no thrombus formation	1109:1136	Thromboelastography detected almost no thrombus formation in the whole blood, mainly on MPE addition.
31892546	2	85	theme	heparan	321:327	arg1	HS					338:339	HS	338:339	HS	338:339	Human heparanase, involved in angiogenesis and metastasis, cleaves heparan sulfate (HS) side chains on the cell surface.
31892546	2	85	theme	heparan	321:327	arg1	sulfate					329:335	heparan sulfate	321:335	heparan sulfate (HS) side chains on the cell surface	321:372	Human heparanase, involved in angiogenesis and metastasis, cleaves heparan sulfate (HS) side chains on the cell surface.
31892546	11	86	from	levels	1276:1281	arg1	effusions					1307:1315	pleural effusions	1299:1315	pleural effusions	1299:1315	Direct measurement revealed high levels of HS chains in pleural effusions.
31892546	4	87	theme	patients	549:556	arg1	Samples					510:516	METHODS Samples	502:516	METHODS Samples of 30 patients with MPE and 44 patients with IPE	502:565	METHODS Samples of 30 patients with MPE and 44 patients with IPE were evaluated in comparison to those of 33 patients with transudate pleural effusions, using heparanase ELISA, heparanase procoagulant activity assay, thrombin and factor Xa chromogenic assays and thromboelastography.
31892546	14	88	theme	cell	1747:1750	arg1	proliferation					1752:1764	tumour cell proliferation	1740:1764	tumour cell proliferation	1740:1764	CONCLUSIONS HS chains released by heparanase form an anticoagulant milieu in MPE, preventing local thrombosis and enabling tumour cell proliferation.
31892546	4	89	theme	Xa	739:740	arg1	assays					754:759	heparanase ELISA, heparanase procoagulant activity assay, thrombin and factor Xa chromogenic assays	661:759	heparanase ELISA, heparanase procoagulant activity assay, thrombin and factor Xa chromogenic assays	661:759	METHODS Samples of 30 patients with MPE and 44 patients with IPE were evaluated in comparison to those of 33 patients with transudate pleural effusions, using heparanase ELISA, heparanase procoagulant activity assay, thrombin and factor Xa chromogenic assays and thromboelastography.
31892546	9	90	theme	whole	1145:1149	arg1	blood					1151:1155	the whole blood	1141:1155	the whole blood	1141:1155	Thromboelastography detected almost no thrombus formation in the whole blood, mainly on MPE addition.
31892546	1	91	theme	treatment	223:231	arg1	options					233:239	current treatment options	215:239	current treatment options	215:239	BACKGROUND While malignant pleural effusion (MPE) is a common and significant cause of morbidity in patients with cancer, current treatment options are limited.
31107248	6	0	theme	microbial	1179:1187	arg1	composition					1189:1199	microbial composition	1179:1199	microbial composition	1179:1199	Transfer of microbiota from 619W mice after the first DSS cycle into treatment-naive 619W mice promoted colitis, indicating that changes in microbial composition enhanced chronic colitis in those animals.
31107248	0	1	theme	microbiota-dependent	73:92	arg1	manner					94:99	a microbiota-dependent manner	71:99	a microbiota-dependent manner	71:99	Protein tyrosine phosphatase non-receptor type 22 modulates colitis in a microbiota-dependent manner.
31107248	4	2	theme	sodium	808:813	arg1	colitis					821:827	acute dextran sulfate sodium (DSS) colitis	786:827	acute dextran sulfate sodium (DSS) colitis	786:827	Here, we demonstrate that mice carrying the PTPN22 variant (619W mice) were protected from acute dextran sulfate sodium (DSS) colitis, but suffered from pronounced inflammation upon chronic DSS treatment.
31107248	1	3	theme	well-balanced	152:164	arg1	interactions					166:177	well-balanced interactions	152:177	well-balanced interactions between the host's immune system and the microbiota	152:229	The gut microbiota is crucial for our health, and well-balanced interactions between the host's immune system and the microbiota are essential to prevent chronic intestinal inflammation, as observed in inflammatory bowel diseases (IBD).
31107248	3	4	dep	shaping	628:634	arg1	microbiota					651:660	the intestinal microbiota	636:660	shaping the intestinal microbiota	628:660	While the role of PTPN22 in modulating molecular pathways involved in IBD pathogenesis is well studied, its impact on shaping the intestinal microbiota has not been addressed in depth.
31107248	2	5	theme	disorders	499:507	arg1	onset					479:483	the onset	475:483	the onset of autoimmune disorders	475:507	A variant in protein tyrosine phosphatase non-receptor type 22 (PTPN22) is associated with reduced risk of developing IBD, but promotes the onset of autoimmune disorders.
31107248	5	6	theme	DSS-induced	969:979	arg1	dysbiosis					981:989	DSS-induced dysbiosis	969:989	DSS-induced dysbiosis	969:989	The basal microbiota composition was distinct between genotypes, and DSS-induced dysbiosis was milder in 619W mice than in WT littermates.
31107248	6	7	theme	619W	1124:1127	arg1	mice					1129:1132	treatment-naive 619W mice	1108:1132	treatment-naive 619W mice	1108:1132	Transfer of microbiota from 619W mice after the first DSS cycle into treatment-naive 619W mice promoted colitis, indicating that changes in microbial composition enhanced chronic colitis in those animals.
31107248	1	8	theme	chronic	256:262	arg1	inflammation					275:286	chronic intestinal inflammation	256:286	chronic intestinal inflammation	256:286	The gut microbiota is crucial for our health, and well-balanced interactions between the host's immune system and the microbiota are essential to prevent chronic intestinal inflammation, as observed in inflammatory bowel diseases (IBD).
31107248	2	9	theme	autoimmune	488:497	arg1	disorders					499:507	autoimmune disorders	488:507	autoimmune disorders	488:507	A variant in protein tyrosine phosphatase non-receptor type 22 (PTPN22) is associated with reduced risk of developing IBD, but promotes the onset of autoimmune disorders.
31107248	6	10	theme	first	1087:1091	arg1	cycle					1097:1101	the first DSS cycle	1083:1101	the first DSS cycle	1083:1101	Transfer of microbiota from 619W mice after the first DSS cycle into treatment-naive 619W mice promoted colitis, indicating that changes in microbial composition enhanced chronic colitis in those animals.
31107248	6	11	theme	treatment-naive	1108:1122	arg1	mice					1129:1132	treatment-naive 619W mice	1108:1132	treatment-naive 619W mice	1108:1132	Transfer of microbiota from 619W mice after the first DSS cycle into treatment-naive 619W mice promoted colitis, indicating that changes in microbial composition enhanced chronic colitis in those animals.
31107248	5	12	theme	basal	904:908	arg1	distinct					937:944	distinct	937:944	distinct	937:944	The basal microbiota composition was distinct between genotypes, and DSS-induced dysbiosis was milder in 619W mice than in WT littermates.
31107248	5	12	theme	basal	904:908	arg1	composition					921:931	The basal microbiota composition	900:931	The basal microbiota composition	900:931	The basal microbiota composition was distinct between genotypes, and DSS-induced dysbiosis was milder in 619W mice than in WT littermates.
31107248	1	13	theme	intestinal	264:273	arg1	inflammation					275:286	chronic intestinal inflammation	256:286	chronic intestinal inflammation	256:286	The gut microbiota is crucial for our health, and well-balanced interactions between the host's immune system and the microbiota are essential to prevent chronic intestinal inflammation, as observed in inflammatory bowel diseases (IBD).
31107248	4	14	theme	acute	786:790	arg1	colitis					821:827	acute dextran sulfate sodium (DSS) colitis	786:827	acute dextran sulfate sodium (DSS) colitis	786:827	Here, we demonstrate that mice carrying the PTPN22 variant (619W mice) were protected from acute dextran sulfate sodium (DSS) colitis, but suffered from pronounced inflammation upon chronic DSS treatment.
31107248	5	15	theme	microbiota	910:919	arg1	distinct					937:944	distinct	937:944	distinct	937:944	The basal microbiota composition was distinct between genotypes, and DSS-induced dysbiosis was milder in 619W mice than in WT littermates.
31107248	5	15	theme	microbiota	910:919	arg1	composition					921:931	The basal microbiota composition	900:931	The basal microbiota composition	900:931	The basal microbiota composition was distinct between genotypes, and DSS-induced dysbiosis was milder in 619W mice than in WT littermates.
31107248	7	16	theme	variant	1287:1293	arg1	presence					1264:1271	presence	1264:1271	presence of the PTPN22 variant	1264:1293	This indicates that presence of the PTPN22 variant affects intestinal inflammation by modulating the host's response to the intestinal microbiota.
31107248	3	17	from	impact	618:623	arg1	shaping					628:634	shaping the intestinal microbiota	628:660	shaping the intestinal microbiota	628:660	While the role of PTPN22 in modulating molecular pathways involved in IBD pathogenesis is well studied, its impact on shaping the intestinal microbiota has not been addressed in depth.
31107248	2	18	from	variant	341:347	arg1	phosphatase					369:379	protein tyrosine phosphatase non-receptor type 22 (PTPN22)	352:409	protein tyrosine phosphatase non-receptor type 22 (PTPN22)	352:409	A variant in protein tyrosine phosphatase non-receptor type 22 (PTPN22) is associated with reduced risk of developing IBD, but promotes the onset of autoimmune disorders.
31107248	2	19	theme	non-receptor	381:392	arg1	type					394:397	non-receptor type 22	381:400	protein tyrosine phosphatase non-receptor type 22 (PTPN22)	352:409	A variant in protein tyrosine phosphatase non-receptor type 22 (PTPN22) is associated with reduced risk of developing IBD, but promotes the onset of autoimmune disorders.
31107248	2	19	theme	non-receptor	381:392	arg1	PTPN22					403:408	PTPN22	403:408	PTPN22	403:408	A variant in protein tyrosine phosphatase non-receptor type 22 (PTPN22) is associated with reduced risk of developing IBD, but promotes the onset of autoimmune disorders.
31107248	0	20	theme	tyrosine	8:15	arg1	phosphatase					17:27	Protein tyrosine phosphatase	0:27	Protein tyrosine phosphatase non-receptor type 22	0:48	Protein tyrosine phosphatase non-receptor type 22 modulates colitis in a microbiota-dependent manner.
31107248	6	21	theme	DSS	1093:1095	arg1	cycle					1097:1101	the first DSS cycle	1083:1101	the first DSS cycle	1083:1101	Transfer of microbiota from 619W mice after the first DSS cycle into treatment-naive 619W mice promoted colitis, indicating that changes in microbial composition enhanced chronic colitis in those animals.
31107248	4	22	theme	sulfate	800:806	arg1	DSS					816:818	DSS	816:818	DSS	816:818	Here, we demonstrate that mice carrying the PTPN22 variant (619W mice) were protected from acute dextran sulfate sodium (DSS) colitis, but suffered from pronounced inflammation upon chronic DSS treatment.
31107248	4	22	theme	sulfate	800:806	arg1	sodium					808:813	dextran sulfate sodium	792:813	acute dextran sulfate sodium (DSS) colitis	786:827	Here, we demonstrate that mice carrying the PTPN22 variant (619W mice) were protected from acute dextran sulfate sodium (DSS) colitis, but suffered from pronounced inflammation upon chronic DSS treatment.
31107248	4	23	theme	DSS	885:887	arg1	treatment					889:897	chronic DSS treatment	877:897	chronic DSS treatment	877:897	Here, we demonstrate that mice carrying the PTPN22 variant (619W mice) were protected from acute dextran sulfate sodium (DSS) colitis, but suffered from pronounced inflammation upon chronic DSS treatment.
31107248	0	24	theme	Protein	0:6	arg1	phosphatase					17:27	Protein tyrosine phosphatase	0:27	Protein tyrosine phosphatase non-receptor type 22	0:48	Protein tyrosine phosphatase non-receptor type 22 modulates colitis in a microbiota-dependent manner.
31107248	4	25	theme	dextran	792:798	arg1	DSS					816:818	DSS	816:818	DSS	816:818	Here, we demonstrate that mice carrying the PTPN22 variant (619W mice) were protected from acute dextran sulfate sodium (DSS) colitis, but suffered from pronounced inflammation upon chronic DSS treatment.
31107248	4	25	theme	dextran	792:798	arg1	sodium					808:813	dextran sulfate sodium	792:813	acute dextran sulfate sodium (DSS) colitis	786:827	Here, we demonstrate that mice carrying the PTPN22 variant (619W mice) were protected from acute dextran sulfate sodium (DSS) colitis, but suffered from pronounced inflammation upon chronic DSS treatment.
31107248	6	26	theme	chronic	1210:1216	arg1	colitis					1218:1224	chronic colitis	1210:1224	chronic colitis in those animals	1210:1241	Transfer of microbiota from 619W mice after the first DSS cycle into treatment-naive 619W mice promoted colitis, indicating that changes in microbial composition enhanced chronic colitis in those animals.
31107248	2	27	theme	tyrosine	360:367	arg1	phosphatase					369:379	protein tyrosine phosphatase non-receptor type 22 (PTPN22)	352:409	protein tyrosine phosphatase non-receptor type 22 (PTPN22)	352:409	A variant in protein tyrosine phosphatase non-receptor type 22 (PTPN22) is associated with reduced risk of developing IBD, but promotes the onset of autoimmune disorders.
31107248	0	28	theme	non-receptor	29:40	arg1	type					42:45	Protein tyrosine phosphatase non-receptor type 22	0:48	Protein tyrosine phosphatase non-receptor type 22	0:48	Protein tyrosine phosphatase non-receptor type 22 modulates colitis in a microbiota-dependent manner.
31107248	4	29	theme	619W	755:758	arg1	variant					746:752	the PTPN22 variant	735:752	the PTPN22 variant (619W mice)	735:764	Here, we demonstrate that mice carrying the PTPN22 variant (619W mice) were protected from acute dextran sulfate sodium (DSS) colitis, but suffered from pronounced inflammation upon chronic DSS treatment.
31107248	4	29	theme	619W	755:758	arg1	mice					760:763	619W mice	755:763	619W mice	755:763	Here, we demonstrate that mice carrying the PTPN22 variant (619W mice) were protected from acute dextran sulfate sodium (DSS) colitis, but suffered from pronounced inflammation upon chronic DSS treatment.
31107248	4	30	theme	pronounced	848:857	arg1	inflammation					859:870	pronounced inflammation	848:870	pronounced inflammation	848:870	Here, we demonstrate that mice carrying the PTPN22 variant (619W mice) were protected from acute dextran sulfate sodium (DSS) colitis, but suffered from pronounced inflammation upon chronic DSS treatment.
31107248	1	31	theme	gut	106:108	arg1	crucial					124:130	crucial	124:130	crucial	124:130	The gut microbiota is crucial for our health, and well-balanced interactions between the host's immune system and the microbiota are essential to prevent chronic intestinal inflammation, as observed in inflammatory bowel diseases (IBD).
31107248	1	31	theme	gut	106:108	arg1	microbiota					110:119	The gut microbiota	102:119	The gut microbiota	102:119	The gut microbiota is crucial for our health, and well-balanced interactions between the host's immune system and the microbiota are essential to prevent chronic intestinal inflammation, as observed in inflammatory bowel diseases (IBD).
31107248	0	32	theme	phosphatase	17:27	arg1	type					42:45	Protein tyrosine phosphatase non-receptor type 22	0:48	Protein tyrosine phosphatase non-receptor type 22	0:48	Protein tyrosine phosphatase non-receptor type 22 modulates colitis in a microbiota-dependent manner.
31107248	7	33	theme	intestinal	1368:1377	arg1	microbiota					1379:1388	the intestinal microbiota	1364:1388	the intestinal microbiota	1364:1388	This indicates that presence of the PTPN22 variant affects intestinal inflammation by modulating the host's response to the intestinal microbiota.
31107248	3	34	theme	PTPN22	528:533	arg1	role					520:523	the role	516:523	the role of PTPN22 in modulating molecular pathways involved in IBD pathogenesis	516:595	While the role of PTPN22 in modulating molecular pathways involved in IBD pathogenesis is well studied, its impact on shaping the intestinal microbiota has not been addressed in depth.
31107248	1	35	theme	immune	198:203	arg1	system					205:210	the host's immune system	187:210	the host's immune system	187:210	The gut microbiota is crucial for our health, and well-balanced interactions between the host's immune system and the microbiota are essential to prevent chronic intestinal inflammation, as observed in inflammatory bowel diseases (IBD).
31107248	4	36	contain	carrying	726:733	arg1	mice					721:724	mice	721:724	mice carrying the PTPN22 variant (619W mice)	721:764	Here, we demonstrate that mice carrying the PTPN22 variant (619W mice) were protected from acute dextran sulfate sodium (DSS) colitis, but suffered from pronounced inflammation upon chronic DSS treatment.
31107248	4	36	contain	carrying	726:733	arg2	mice					760:763	619W mice	755:763	619W mice	755:763	Here, we demonstrate that mice carrying the PTPN22 variant (619W mice) were protected from acute dextran sulfate sodium (DSS) colitis, but suffered from pronounced inflammation upon chronic DSS treatment.
31107248	4	36	contain	carrying	726:733	arg2	variant					746:752	the PTPN22 variant	735:752	the PTPN22 variant (619W mice)	735:764	Here, we demonstrate that mice carrying the PTPN22 variant (619W mice) were protected from acute dextran sulfate sodium (DSS) colitis, but suffered from pronounced inflammation upon chronic DSS treatment.
31107248	1	37	theme	inflammatory	304:315	arg1	IBD					333:335	IBD	333:335	IBD	333:335	The gut microbiota is crucial for our health, and well-balanced interactions between the host's immune system and the microbiota are essential to prevent chronic intestinal inflammation, as observed in inflammatory bowel diseases (IBD).
31107248	1	37	theme	inflammatory	304:315	arg1	diseases					323:330	inflammatory bowel diseases	304:330	inflammatory bowel diseases (IBD)	304:336	The gut microbiota is crucial for our health, and well-balanced interactions between the host's immune system and the microbiota are essential to prevent chronic intestinal inflammation, as observed in inflammatory bowel diseases (IBD).
31107248	5	38	theme	WT	1023:1024	arg1	littermates					1026:1036	WT littermates	1023:1036	WT littermates	1023:1036	The basal microbiota composition was distinct between genotypes, and DSS-induced dysbiosis was milder in 619W mice than in WT littermates.
31107248	3	39	theme	molecular	549:557	arg1	pathways					559:566	molecular pathways	549:566	molecular pathways involved in IBD pathogenesis	549:595	While the role of PTPN22 in modulating molecular pathways involved in IBD pathogenesis is well studied, its impact on shaping the intestinal microbiota has not been addressed in depth.
31107248	1	40	theme	bowel	317:321	arg1	IBD					333:335	IBD	333:335	IBD	333:335	The gut microbiota is crucial for our health, and well-balanced interactions between the host's immune system and the microbiota are essential to prevent chronic intestinal inflammation, as observed in inflammatory bowel diseases (IBD).
31107248	1	40	theme	bowel	317:321	arg1	diseases					323:330	inflammatory bowel diseases	304:330	inflammatory bowel diseases (IBD)	304:336	The gut microbiota is crucial for our health, and well-balanced interactions between the host's immune system and the microbiota are essential to prevent chronic intestinal inflammation, as observed in inflammatory bowel diseases (IBD).
31107248	6	41	from	mice	1072:1075	arg1	Transfer					1039:1046	Transfer	1039:1046	Transfer of microbiota from 619W mice after the first DSS cycle into treatment-naive 619W mice	1039:1132	Transfer of microbiota from 619W mice after the first DSS cycle into treatment-naive 619W mice promoted colitis, indicating that changes in microbial composition enhanced chronic colitis in those animals.
31107248	6	42	theme	619W	1067:1070	arg1	mice					1072:1075	619W mice	1067:1075	619W mice after the first DSS cycle	1067:1101	Transfer of microbiota from 619W mice after the first DSS cycle into treatment-naive 619W mice promoted colitis, indicating that changes in microbial composition enhanced chronic colitis in those animals.
31107248	3	43	theme	intestinal	640:649	arg1	microbiota					651:660	the intestinal microbiota	636:660	shaping the intestinal microbiota	628:660	While the role of PTPN22 in modulating molecular pathways involved in IBD pathogenesis is well studied, its impact on shaping the intestinal microbiota has not been addressed in depth.
31107248	2	44	dep	phosphatase	369:379	arg1	type					394:397	non-receptor type 22	381:400	protein tyrosine phosphatase non-receptor type 22 (PTPN22)	352:409	A variant in protein tyrosine phosphatase non-receptor type 22 (PTPN22) is associated with reduced risk of developing IBD, but promotes the onset of autoimmune disorders.
31107248	2	44	dep	phosphatase	369:379	arg1	PTPN22					403:408	PTPN22	403:408	PTPN22	403:408	A variant in protein tyrosine phosphatase non-receptor type 22 (PTPN22) is associated with reduced risk of developing IBD, but promotes the onset of autoimmune disorders.
31107248	7	45	theme	PTPN22	1280:1285	arg1	variant					1287:1293	the PTPN22 variant	1276:1293	the PTPN22 variant	1276:1293	This indicates that presence of the PTPN22 variant affects intestinal inflammation by modulating the host's response to the intestinal microbiota.
31107248	4	46	theme	PTPN22	739:744	arg1	variant					746:752	the PTPN22 variant	735:752	the PTPN22 variant (619W mice)	735:764	Here, we demonstrate that mice carrying the PTPN22 variant (619W mice) were protected from acute dextran sulfate sodium (DSS) colitis, but suffered from pronounced inflammation upon chronic DSS treatment.
31107248	4	46	theme	PTPN22	739:744	arg1	mice					760:763	619W mice	755:763	619W mice	755:763	Here, we demonstrate that mice carrying the PTPN22 variant (619W mice) were protected from acute dextran sulfate sodium (DSS) colitis, but suffered from pronounced inflammation upon chronic DSS treatment.
31107248	6	47	theme	microbiota	1051:1060	arg1	Transfer					1039:1046	Transfer	1039:1046	Transfer of microbiota from 619W mice after the first DSS cycle into treatment-naive 619W mice	1039:1132	Transfer of microbiota from 619W mice after the first DSS cycle into treatment-naive 619W mice promoted colitis, indicating that changes in microbial composition enhanced chronic colitis in those animals.
31107248	6	48	from	colitis	1218:1224	arg1	animals					1235:1241	those animals	1229:1241	those animals	1229:1241	Transfer of microbiota from 619W mice after the first DSS cycle into treatment-naive 619W mice promoted colitis, indicating that changes in microbial composition enhanced chronic colitis in those animals.
31107248	5	49	theme	619W	1005:1008	arg1	mice					1010:1013	619W mice	1005:1013	619W mice	1005:1013	The basal microbiota composition was distinct between genotypes, and DSS-induced dysbiosis was milder in 619W mice than in WT littermates.
31107248	3	50	theme	IBD	580:582	arg1	pathogenesis					584:595	IBD pathogenesis	580:595	IBD pathogenesis	580:595	While the role of PTPN22 in modulating molecular pathways involved in IBD pathogenesis is well studied, its impact on shaping the intestinal microbiota has not been addressed in depth.
31107248	2	51	theme	protein	352:358	arg1	phosphatase					369:379	protein tyrosine phosphatase non-receptor type 22 (PTPN22)	352:409	protein tyrosine phosphatase non-receptor type 22 (PTPN22)	352:409	A variant in protein tyrosine phosphatase non-receptor type 22 (PTPN22) is associated with reduced risk of developing IBD, but promotes the onset of autoimmune disorders.
31107248	6	52	from	changes	1168:1174	arg1	composition					1189:1199	microbial composition	1179:1199	microbial composition	1179:1199	Transfer of microbiota from 619W mice after the first DSS cycle into treatment-naive 619W mice promoted colitis, indicating that changes in microbial composition enhanced chronic colitis in those animals.
31107248	7	53	theme	intestinal	1303:1312	arg1	inflammation					1314:1325	intestinal inflammation	1303:1325	intestinal inflammation	1303:1325	This indicates that presence of the PTPN22 variant affects intestinal inflammation by modulating the host's response to the intestinal microbiota.
31107248	2	54	theme	reduced	430:436	arg1	risk					438:441	reduced risk	430:441	reduced risk of developing IBD	430:459	A variant in protein tyrosine phosphatase non-receptor type 22 (PTPN22) is associated with reduced risk of developing IBD, but promotes the onset of autoimmune disorders.
31107248	4	55	theme	chronic	877:883	arg1	treatment					889:897	chronic DSS treatment	877:897	chronic DSS treatment	877:897	Here, we demonstrate that mice carrying the PTPN22 variant (619W mice) were protected from acute dextran sulfate sodium (DSS) colitis, but suffered from pronounced inflammation upon chronic DSS treatment.
30054355	4	0	with	identity	1163:1170	arg1	enzymes					1207:1213	the available GH79 GUS enzymes	1184:1213	the available GH79 GUS enzymes	1184:1213	The gene for TpGUS79A was then cloned and verified by heterologous expression in Pichia pastoris TpGUS79A belonged to glycoside hydrolase family 79 (GH79) but shared low amino acid sequence identity (<35%) with the available GH79 GUS enzymes.
30054355	4	1	theme	heterologous	1027:1038	arg1	expression					1040:1049	heterologous expression	1027:1049	heterologous expression in Pichia pastoris TpGUS79A belonged to glycoside hydrolase family 79 (GH79) but shared low amino acid sequence identity (<35%) with the available GH79 GUS enzymes	1027:1213	The gene for TpGUS79A was then cloned and verified by heterologous expression in Pichia pastoris TpGUS79A belonged to glycoside hydrolase family 79 (GH79) but shared low amino acid sequence identity (<35%) with the available GH79 GUS enzymes.
30054355	2	2	theme	acid	598:601	arg1	formation					555:563	the formation	551:563	the formation of the by-product glycyrrhetinic acid (GA) from the further hydrolysis of GAMG	551:642	In this study, we isolated a β-glucuronidase (TpGUS79A) from the filamentous fungus Talaromyces pinophilus Li-93 that can specifically and precisely convert GL to GAMG without the formation of the by-product glycyrrhetinic acid (GA) from the further hydrolysis of GAMG.
30054355	10	3	theme	environmentally	2009:2023	arg1	friendly					2025:2032	environmentally friendly	2009:2032	environmentally friendly	2009:2032	Therefore, TpGUS79A is potentially a powerful biocatalyst for environmentally friendly and cost-effective production of GAMG.IMPORTANCE Compared to chemical methods, the biotransformation of glycyrrhizin (GL) into glycyrrhetinic acid 3-O-mono-β-d-glucuronide (GAMG), which has a higher sweetness and stronger pharmacological activity than those of GL, via catalysis by β-glucuronidase is an environmentally friendly approach due to the mild reaction conditions and the high yield of GAMG.
30054355	3	4	theme	desorption	719:728	arg1	time					748:751	matrix-assisted laser desorption ionization-tandem time	697:751	matrix-assisted laser desorption ionization-tandem time of flight mass spectrometry (MALDI-TOF-TOF MS) and deglycosylation	697:818	First, TpGUS79A was purified and identified through matrix-assisted laser desorption ionization-tandem time of flight mass spectrometry (MALDI-TOF-TOF MS) and deglycosylation, indicating that TpGUS79A is a highly N-glycosylated monomeric protein with a molecular mass of around 85 kDa, including around 25 kDa of glycan moiety.
30054355	8	5	theme	substrate	1787:1795	arg1	recognition					1797:1807	substrate recognition	1787:1807	substrate recognition	1787:1807	Homology modeling, substrate docking, and sequence alignment were employed to identify the key residues for substrate recognition.
30054355	2	6	theme	further	617:623	arg1	hydrolysis					625:634	the further hydrolysis	613:634	the further hydrolysis of GAMG	613:642	In this study, we isolated a β-glucuronidase (TpGUS79A) from the filamentous fungus Talaromyces pinophilus Li-93 that can specifically and precisely convert GL to GAMG without the formation of the by-product glycyrrhetinic acid (GA) from the further hydrolysis of GAMG.
30054355	10	7	theme	GAMG.IMPORTANCE	2067:2081	arg1	friendly					2025:2032	environmentally friendly	2009:2032	environmentally friendly	2009:2032	Therefore, TpGUS79A is potentially a powerful biocatalyst for environmentally friendly and cost-effective production of GAMG.IMPORTANCE Compared to chemical methods, the biotransformation of glycyrrhizin (GL) into glycyrrhetinic acid 3-O-mono-β-d-glucuronide (GAMG), which has a higher sweetness and stronger pharmacological activity than those of GL, via catalysis by β-glucuronidase is an environmentally friendly approach due to the mild reaction conditions and the high yield of GAMG.
30054355	10	7	theme	GAMG.IMPORTANCE	2067:2081	arg1	production					2053:2062	cost-effective production	2038:2062	cost-effective production	2038:2062	Therefore, TpGUS79A is potentially a powerful biocatalyst for environmentally friendly and cost-effective production of GAMG.IMPORTANCE Compared to chemical methods, the biotransformation of glycyrrhizin (GL) into glycyrrhetinic acid 3-O-mono-β-d-glucuronide (GAMG), which has a higher sweetness and stronger pharmacological activity than those of GL, via catalysis by β-glucuronidase is an environmentally friendly approach due to the mild reaction conditions and the high yield of GAMG.
30054355	3	8	theme	matrix-assisted	697:711	arg1	time					748:751	matrix-assisted laser desorption ionization-tandem time	697:751	matrix-assisted laser desorption ionization-tandem time of flight mass spectrometry (MALDI-TOF-TOF MS) and deglycosylation	697:818	First, TpGUS79A was purified and identified through matrix-assisted laser desorption ionization-tandem time of flight mass spectrometry (MALDI-TOF-TOF MS) and deglycosylation, indicating that TpGUS79A is a highly N-glycosylated monomeric protein with a molecular mass of around 85 kDa, including around 25 kDa of glycan moiety.
30054355	1	9	theme	higher	214:219	arg1	sweetness					221:229	a higher sweetness	212:229	a higher sweetness	212:229	Glycyrrhetinic acid 3-O-mono-β-d-glucuronide (GAMG), which possesses a higher sweetness and stronger pharmacological activity than those of glycyrrhizin (GL), can be obtained by removal of the distal glucuronic acid (GlcA) from GL.
30054355	10	10	theme	stronger	2247:2254	arg1	activity					2272:2279	stronger pharmacological activity	2247:2279	stronger pharmacological activity	2247:2279	Therefore, TpGUS79A is potentially a powerful biocatalyst for environmentally friendly and cost-effective production of GAMG.IMPORTANCE Compared to chemical methods, the biotransformation of glycyrrhizin (GL) into glycyrrhetinic acid 3-O-mono-β-d-glucuronide (GAMG), which has a higher sweetness and stronger pharmacological activity than those of GL, via catalysis by β-glucuronidase is an environmentally friendly approach due to the mild reaction conditions and the high yield of GAMG.
30054355	7	11	from	case	1659:1662	arg1	acid					1599:1602	the artificial substrate pNP β-glucopyranosiduronic acid	1547:1602	the artificial substrate pNP β-glucopyranosiduronic acid (kcat/K of 0.01 mM-1 s-1)	1547:1628	TpGUS79A showed a much higher catalytic efficiency on GL (kcat/K of 11.14 mM-1 s-1) than on the artificial substrate pNP β-glucopyranosiduronic acid (kcat/K of 0.01 mM-1 s-1), which is different from the case for most GUSs.
30054355	7	11	from	case	1659:1662	arg1	different					1640:1648	different	1640:1648	different	1640:1648	TpGUS79A showed a much higher catalytic efficiency on GL (kcat/K of 11.14 mM-1 s-1) than on the artificial substrate pNP β-glucopyranosiduronic acid (kcat/K of 0.01 mM-1 s-1), which is different from the case for most GUSs.
30054355	10	12	theme	GAMG	2430:2433	arg1	conditions					2397:2406	the mild reaction conditions	2379:2406	the mild reaction conditions	2379:2406	Therefore, TpGUS79A is potentially a powerful biocatalyst for environmentally friendly and cost-effective production of GAMG.IMPORTANCE Compared to chemical methods, the biotransformation of glycyrrhizin (GL) into glycyrrhetinic acid 3-O-mono-β-d-glucuronide (GAMG), which has a higher sweetness and stronger pharmacological activity than those of GL, via catalysis by β-glucuronidase is an environmentally friendly approach due to the mild reaction conditions and the high yield of GAMG.
30054355	10	12	theme	GAMG	2430:2433	arg1	yield					2421:2425	the high yield	2412:2425	the high yield of GAMG	2412:2433	Therefore, TpGUS79A is potentially a powerful biocatalyst for environmentally friendly and cost-effective production of GAMG.IMPORTANCE Compared to chemical methods, the biotransformation of glycyrrhizin (GL) into glycyrrhetinic acid 3-O-mono-β-d-glucuronide (GAMG), which has a higher sweetness and stronger pharmacological activity than those of GL, via catalysis by β-glucuronidase is an environmentally friendly approach due to the mild reaction conditions and the high yield of GAMG.
30054355	3	13	theme	flight	756:761	arg1	spectrometry					768:779	flight mass spectrometry	756:779	flight mass spectrometry (MALDI-TOF-TOF MS)	756:798	First, TpGUS79A was purified and identified through matrix-assisted laser desorption ionization-tandem time of flight mass spectrometry (MALDI-TOF-TOF MS) and deglycosylation, indicating that TpGUS79A is a highly N-glycosylated monomeric protein with a molecular mass of around 85 kDa, including around 25 kDa of glycan moiety.
30054355	3	13	theme	flight	756:761	arg1	MS					796:797	MALDI-TOF-TOF MS	782:797	MALDI-TOF-TOF MS	782:797	First, TpGUS79A was purified and identified through matrix-assisted laser desorption ionization-tandem time of flight mass spectrometry (MALDI-TOF-TOF MS) and deglycosylation, indicating that TpGUS79A is a highly N-glycosylated monomeric protein with a molecular mass of around 85 kDa, including around 25 kDa of glycan moiety.
30054355	12	14	theme	novel	2702:2706	arg1	β-glucuronidase					2708:2722	a novel β-glucuronidase	2700:2722	a novel β-glucuronidase (named TpGUS79A)	2700:2739	In the present study, we succeeded in isolating a novel β-glucuronidase (named TpGUS79A) from Talaromyces pinophilus Li-93 that specifically hydrolyzes GL to GAMG without the formation of GA.
30054355	14	15	theme	cost-effective	3110:3123	arg1	production					3125:3134	the cost-effective production	3106:3134	the cost-effective production of GAMG through GL transformation	3106:3168	Moreover, the gene for TpGUS79A was cloned and its function verified by heterologous expression in P. pastoris Therefore, TpGUS79A can serve as a powerful biocatalyst for the cost-effective production of GAMG through GL transformation.
30054355	4	16	theme	glycoside	1091:1099	arg1	family					1111:1116	glycoside hydrolase family 79	1091:1119	glycoside hydrolase family 79 (GH79)	1091:1126	The gene for TpGUS79A was then cloned and verified by heterologous expression in Pichia pastoris TpGUS79A belonged to glycoside hydrolase family 79 (GH79) but shared low amino acid sequence identity (<35%) with the available GH79 GUS enzymes.
30054355	4	16	theme	glycoside	1091:1099	arg1	GH79					1122:1125	GH79	1122:1125	GH79	1122:1125	The gene for TpGUS79A was then cloned and verified by heterologous expression in Pichia pastoris TpGUS79A belonged to glycoside hydrolase family 79 (GH79) but shared low amino acid sequence identity (<35%) with the available GH79 GUS enzymes.
30054355	14	17	theme	heterologous	3007:3018	arg1	expression					3020:3029	heterologous expression	3007:3029	heterologous expression in P. pastoris Therefore, TpGUS79A can serve as a powerful biocatalyst for the cost-effective production of GAMG through GL transformation	3007:3168	Moreover, the gene for TpGUS79A was cloned and its function verified by heterologous expression in P. pastoris Therefore, TpGUS79A can serve as a powerful biocatalyst for the cost-effective production of GAMG through GL transformation.
30054355	1	18	theme	pharmacological	244:258	arg1	activity					260:267	stronger pharmacological activity	235:267	stronger pharmacological activity	235:267	Glycyrrhetinic acid 3-O-mono-β-d-glucuronide (GAMG), which possesses a higher sweetness and stronger pharmacological activity than those of glycyrrhizin (GL), can be obtained by removal of the distal glucuronic acid (GlcA) from GL.
30054355	7	19	theme	β-glucopyranosiduronic	1576:1597	arg1	acid					1599:1602	the artificial substrate pNP β-glucopyranosiduronic acid	1547:1602	the artificial substrate pNP β-glucopyranosiduronic acid (kcat/K of 0.01 mM-1 s-1)	1547:1628	TpGUS79A showed a much higher catalytic efficiency on GL (kcat/K of 11.14 mM-1 s-1) than on the artificial substrate pNP β-glucopyranosiduronic acid (kcat/K of 0.01 mM-1 s-1), which is different from the case for most GUSs.
30054355	7	19	theme	β-glucopyranosiduronic	1576:1597	arg1	different					1640:1648	different	1640:1648	different	1640:1648	TpGUS79A showed a much higher catalytic efficiency on GL (kcat/K of 11.14 mM-1 s-1) than on the artificial substrate pNP β-glucopyranosiduronic acid (kcat/K of 0.01 mM-1 s-1), which is different from the case for most GUSs.
30054355	12	20	theme	present	2659:2665	arg1	study					2667:2671	the present study	2655:2671	the present study	2655:2671	In the present study, we succeeded in isolating a novel β-glucuronidase (named TpGUS79A) from Talaromyces pinophilus Li-93 that specifically hydrolyzes GL to GAMG without the formation of GA.
30054355	7	21	theme	most	1668:1671	arg1	GUSs					1673:1676	most GUSs	1668:1676	most GUSs	1668:1676	TpGUS79A showed a much higher catalytic efficiency on GL (kcat/K of 11.14 mM-1 s-1) than on the artificial substrate pNP β-glucopyranosiduronic acid (kcat/K of 0.01 mM-1 s-1), which is different from the case for most GUSs.
30054355	3	22	theme	MALDI-TOF-TOF	782:794	arg1	spectrometry					768:779	flight mass spectrometry	756:779	flight mass spectrometry (MALDI-TOF-TOF MS)	756:798	First, TpGUS79A was purified and identified through matrix-assisted laser desorption ionization-tandem time of flight mass spectrometry (MALDI-TOF-TOF MS) and deglycosylation, indicating that TpGUS79A is a highly N-glycosylated monomeric protein with a molecular mass of around 85 kDa, including around 25 kDa of glycan moiety.
30054355	3	22	theme	MALDI-TOF-TOF	782:794	arg1	MS					796:797	MALDI-TOF-TOF MS	782:797	MALDI-TOF-TOF MS	782:797	First, TpGUS79A was purified and identified through matrix-assisted laser desorption ionization-tandem time of flight mass spectrometry (MALDI-TOF-TOF MS) and deglycosylation, indicating that TpGUS79A is a highly N-glycosylated monomeric protein with a molecular mass of around 85 kDa, including around 25 kDa of glycan moiety.
30054355	8	23	theme	Homology	1679:1686	arg1	modeling					1688:1695	Homology modeling	1679:1695	Homology modeling	1679:1695	Homology modeling, substrate docking, and sequence alignment were employed to identify the key residues for substrate recognition.
30054355	14	24	from	expression	3020:3029	arg1	pastoris					3037:3044	P. pastoris Therefore, TpGUS79A can serve as a powerful biocatalyst for the cost-effective production of GAMG through GL transformation	3034:3168	P. pastoris Therefore, TpGUS79A can serve as a powerful biocatalyst for the cost-effective production of GAMG through GL transformation	3034:3168	Moreover, the gene for TpGUS79A was cloned and its function verified by heterologous expression in P. pastoris Therefore, TpGUS79A can serve as a powerful biocatalyst for the cost-effective production of GAMG through GL transformation.
30054355	7	25	theme	substrate	1562:1570	arg1	acid					1599:1602	the artificial substrate pNP β-glucopyranosiduronic acid	1547:1602	the artificial substrate pNP β-glucopyranosiduronic acid (kcat/K of 0.01 mM-1 s-1)	1547:1628	TpGUS79A showed a much higher catalytic efficiency on GL (kcat/K of 11.14 mM-1 s-1) than on the artificial substrate pNP β-glucopyranosiduronic acid (kcat/K of 0.01 mM-1 s-1), which is different from the case for most GUSs.
30054355	7	25	theme	substrate	1562:1570	arg1	different					1640:1648	different	1640:1648	different	1640:1648	TpGUS79A showed a much higher catalytic efficiency on GL (kcat/K of 11.14 mM-1 s-1) than on the artificial substrate pNP β-glucopyranosiduronic acid (kcat/K of 0.01 mM-1 s-1), which is different from the case for most GUSs.
30054355	2	26	theme	by-product	572:581	arg1	GA					604:605	GA	604:605	GA	604:605	In this study, we isolated a β-glucuronidase (TpGUS79A) from the filamentous fungus Talaromyces pinophilus Li-93 that can specifically and precisely convert GL to GAMG without the formation of the by-product glycyrrhetinic acid (GA) from the further hydrolysis of GAMG.
30054355	2	26	theme	by-product	572:581	arg1	acid					598:601	the by-product glycyrrhetinic acid	568:601	the by-product glycyrrhetinic acid (GA) from the further hydrolysis of GAMG	568:642	In this study, we isolated a β-glucuronidase (TpGUS79A) from the filamentous fungus Talaromyces pinophilus Li-93 that can specifically and precisely convert GL to GAMG without the formation of the by-product glycyrrhetinic acid (GA) from the further hydrolysis of GAMG.
30054355	11	27	theme	low	2475:2477	arg1	specificity					2489:2499	low substrate specificity	2475:2499	low substrate specificity toward GL	2475:2509	However, currently available GUSs show low substrate specificity toward GL and further hydrolyze GAMG to glycyrrhetinic acid (GA) as a by-product, increasing the difficulty of subsequent separation and purification.
30054355	4	28	theme	sequence	1154:1161	arg1	identity					1163:1170	low amino acid sequence identity	1139:1170	low amino acid sequence identity (<35%) with the available GH79 GUS enzymes	1139:1213	The gene for TpGUS79A was then cloned and verified by heterologous expression in Pichia pastoris TpGUS79A belonged to glycoside hydrolase family 79 (GH79) but shared low amino acid sequence identity (<35%) with the available GH79 GUS enzymes.
30054355	4	28	theme	sequence	1154:1161	arg1	%					1176:1176	<35%	1173:1176	<35%	1173:1176	The gene for TpGUS79A was then cloned and verified by heterologous expression in Pichia pastoris TpGUS79A belonged to glycoside hydrolase family 79 (GH79) but shared low amino acid sequence identity (<35%) with the available GH79 GUS enzymes.
30054355	3	29	theme	spectrometry	768:779	arg1	time					748:751	matrix-assisted laser desorption ionization-tandem time	697:751	matrix-assisted laser desorption ionization-tandem time of flight mass spectrometry (MALDI-TOF-TOF MS) and deglycosylation	697:818	First, TpGUS79A was purified and identified through matrix-assisted laser desorption ionization-tandem time of flight mass spectrometry (MALDI-TOF-TOF MS) and deglycosylation, indicating that TpGUS79A is a highly N-glycosylated monomeric protein with a molecular mass of around 85 kDa, including around 25 kDa of glycan moiety.
30054355	1	30	theme	3-O-mono-β-d-glucuronide	163:186	arg1	acid					158:161	Glycyrrhetinic acid 3-O-mono-β-d-glucuronide	143:186	Glycyrrhetinic acid 3-O-mono-β-d-glucuronide (GAMG)	143:193	Glycyrrhetinic acid 3-O-mono-β-d-glucuronide (GAMG), which possesses a higher sweetness and stronger pharmacological activity than those of glycyrrhizin (GL), can be obtained by removal of the distal glucuronic acid (GlcA) from GL.
30054355	1	30	theme	3-O-mono-β-d-glucuronide	163:186	arg1	GAMG					189:192	GAMG	189:192	GAMG	189:192	Glycyrrhetinic acid 3-O-mono-β-d-glucuronide (GAMG), which possesses a higher sweetness and stronger pharmacological activity than those of glycyrrhizin (GL), can be obtained by removal of the distal glucuronic acid (GlcA) from GL.
30054355	3	31	theme	N-glycosylated	858:871	arg1	TpGUS79A					837:844	TpGUS79A	837:844	TpGUS79A	837:844	First, TpGUS79A was purified and identified through matrix-assisted laser desorption ionization-tandem time of flight mass spectrometry (MALDI-TOF-TOF MS) and deglycosylation, indicating that TpGUS79A is a highly N-glycosylated monomeric protein with a molecular mass of around 85 kDa, including around 25 kDa of glycan moiety.
30054355	3	31	theme	N-glycosylated	858:871	arg1	protein					883:889	a highly N-glycosylated monomeric protein	849:889	a highly N-glycosylated monomeric protein with a molecular mass of around 85 kDa, including around 25 kDa of glycan moiety	849:970	First, TpGUS79A was purified and identified through matrix-assisted laser desorption ionization-tandem time of flight mass spectrometry (MALDI-TOF-TOF MS) and deglycosylation, indicating that TpGUS79A is a highly N-glycosylated monomeric protein with a molecular mass of around 85 kDa, including around 25 kDa of glycan moiety.
30054355	9	32	theme	GL	1909:1910	arg1	hydrolysis					1912:1921	GL hydrolysis	1909:1921	GL hydrolysis by T. pinophilus Li-93	1909:1944	Finally, a fed-batch fermentation in a 150-liter fermentor was established to prepare GAMG through GL hydrolysis by T. pinophilus Li-93.
30054355	11	33	theme	subsequent	2612:2621	arg1	separation					2623:2632	subsequent separation	2612:2632	subsequent separation	2612:2632	However, currently available GUSs show low substrate specificity toward GL and further hydrolyze GAMG to glycyrrhetinic acid (GA) as a by-product, increasing the difficulty of subsequent separation and purification.
30054355	3	34	theme	deglycosylation	804:818	arg1	time					748:751	matrix-assisted laser desorption ionization-tandem time	697:751	matrix-assisted laser desorption ionization-tandem time of flight mass spectrometry (MALDI-TOF-TOF MS) and deglycosylation	697:818	First, TpGUS79A was purified and identified through matrix-assisted laser desorption ionization-tandem time of flight mass spectrometry (MALDI-TOF-TOF MS) and deglycosylation, indicating that TpGUS79A is a highly N-glycosylated monomeric protein with a molecular mass of around 85 kDa, including around 25 kDa of glycan moiety.
30054355	5	35	contain	had	1225:1227	arg2	specificity					1282:1292	poor specificity	1277:1292	poor specificity toward the aglycone moiety	1277:1319	TpGUS79A had strict specificity toward the glycan moiety but poor specificity toward the aglycone moiety.
30054355	5	35	contain	had	1225:1227	arg1	TpGUS79A					1216:1223	TpGUS79A	1216:1223	TpGUS79A	1216:1223	TpGUS79A had strict specificity toward the glycan moiety but poor specificity toward the aglycone moiety.
30054355	5	35	contain	had	1225:1227	arg2	specificity					1236:1246	strict specificity	1229:1246	strict specificity toward the glycan moiety	1229:1271	TpGUS79A had strict specificity toward the glycan moiety but poor specificity toward the aglycone moiety.
30054355	10	36	theme	environmentally	2338:2352	arg1	approach					2363:2370	an environmentally friendly approach	2335:2370	an environmentally friendly approach due to the mild reaction conditions and the high yield of GAMG	2335:2433	Therefore, TpGUS79A is potentially a powerful biocatalyst for environmentally friendly and cost-effective production of GAMG.IMPORTANCE Compared to chemical methods, the biotransformation of glycyrrhizin (GL) into glycyrrhetinic acid 3-O-mono-β-d-glucuronide (GAMG), which has a higher sweetness and stronger pharmacological activity than those of GL, via catalysis by β-glucuronidase is an environmentally friendly approach due to the mild reaction conditions and the high yield of GAMG.
30054355	10	36	theme	environmentally	2338:2352	arg1	biotransformation					2117:2133	the biotransformation	2113:2133	the biotransformation of glycyrrhizin (GL) into glycyrrhetinic acid 3-O-mono-β-d-glucuronide (GAMG), which has a higher sweetness and stronger pharmacological activity than those of GL, via catalysis by β-glucuronidase	2113:2330	Therefore, TpGUS79A is potentially a powerful biocatalyst for environmentally friendly and cost-effective production of GAMG.IMPORTANCE Compared to chemical methods, the biotransformation of glycyrrhizin (GL) into glycyrrhetinic acid 3-O-mono-β-d-glucuronide (GAMG), which has a higher sweetness and stronger pharmacological activity than those of GL, via catalysis by β-glucuronidase is an environmentally friendly approach due to the mild reaction conditions and the high yield of GAMG.
30054355	5	37	theme	glycan	1259:1264	arg1	moiety					1266:1271	the glycan moiety	1255:1271	the glycan moiety	1255:1271	TpGUS79A had strict specificity toward the glycan moiety but poor specificity toward the aglycone moiety.
30054355	3	38	with	protein	883:889	arg1	mass					908:911	a molecular mass	896:911	a molecular mass	896:911	First, TpGUS79A was purified and identified through matrix-assisted laser desorption ionization-tandem time of flight mass spectrometry (MALDI-TOF-TOF MS) and deglycosylation, indicating that TpGUS79A is a highly N-glycosylated monomeric protein with a molecular mass of around 85 kDa, including around 25 kDa of glycan moiety.
30054355	6	39	theme	GL	1402:1403	arg1	bond					1394:1397	the distal glucuronic bond	1372:1397	the distal glucuronic bond of GL	1372:1403	Interestingly, TpGUS79A recognized and hydrolyzed the distal glucuronic bond of GL but could not cleave the glucuronic bond in GAMG.
30054355	7	40	theme	s-1	1534:1536	arg1	kcat/K					1513:1518	kcat/K	1513:1518	kcat/K of 11.14 mM-1 s-1	1513:1536	TpGUS79A showed a much higher catalytic efficiency on GL (kcat/K of 11.14 mM-1 s-1) than on the artificial substrate pNP β-glucopyranosiduronic acid (kcat/K of 0.01 mM-1 s-1), which is different from the case for most GUSs.
30054355	4	41	theme	amino	1143:1147	arg1	identity					1163:1170	low amino acid sequence identity	1139:1170	low amino acid sequence identity (<35%) with the available GH79 GUS enzymes	1139:1213	The gene for TpGUS79A was then cloned and verified by heterologous expression in Pichia pastoris TpGUS79A belonged to glycoside hydrolase family 79 (GH79) but shared low amino acid sequence identity (<35%) with the available GH79 GUS enzymes.
30054355	4	41	theme	amino	1143:1147	arg1	%					1176:1176	<35%	1173:1176	<35%	1173:1176	The gene for TpGUS79A was then cloned and verified by heterologous expression in Pichia pastoris TpGUS79A belonged to glycoside hydrolase family 79 (GH79) but shared low amino acid sequence identity (<35%) with the available GH79 GUS enzymes.
30054355	1	42	contain	possesses	202:210	arg1	acid					158:161	Glycyrrhetinic acid 3-O-mono-β-d-glucuronide	143:186	Glycyrrhetinic acid 3-O-mono-β-d-glucuronide (GAMG)	143:193	Glycyrrhetinic acid 3-O-mono-β-d-glucuronide (GAMG), which possesses a higher sweetness and stronger pharmacological activity than those of glycyrrhizin (GL), can be obtained by removal of the distal glucuronic acid (GlcA) from GL.
30054355	1	42	contain	possesses	202:210	arg2	activity					260:267	stronger pharmacological activity	235:267	stronger pharmacological activity	235:267	Glycyrrhetinic acid 3-O-mono-β-d-glucuronide (GAMG), which possesses a higher sweetness and stronger pharmacological activity than those of glycyrrhizin (GL), can be obtained by removal of the distal glucuronic acid (GlcA) from GL.
30054355	1	42	contain	possesses	202:210	arg1	GAMG					189:192	GAMG	189:192	GAMG	189:192	Glycyrrhetinic acid 3-O-mono-β-d-glucuronide (GAMG), which possesses a higher sweetness and stronger pharmacological activity than those of glycyrrhizin (GL), can be obtained by removal of the distal glucuronic acid (GlcA) from GL.
30054355	1	42	contain	possesses	202:210	arg2	sweetness					221:229	a higher sweetness	212:229	a higher sweetness	212:229	Glycyrrhetinic acid 3-O-mono-β-d-glucuronide (GAMG), which possesses a higher sweetness and stronger pharmacological activity than those of glycyrrhizin (GL), can be obtained by removal of the distal glucuronic acid (GlcA) from GL.
30054355	13	43	from	activity	2871:2878	arg1	GL					2883:2884	GL	2883:2884	GL	2883:2884	TpGUS79A also shows higher activity on GL than those of the previously characterized GUSs.
30054355	7	44	dep	acid	1599:1602	arg1	kcat/K					1605:1610	kcat/K	1605:1610	kcat/K of 0.01 mM-1 s-1	1605:1627	TpGUS79A showed a much higher catalytic efficiency on GL (kcat/K of 11.14 mM-1 s-1) than on the artificial substrate pNP β-glucopyranosiduronic acid (kcat/K of 0.01 mM-1 s-1), which is different from the case for most GUSs.
30054355	2	45	dep	filamentous	440:450	arg1	fungus					452:457	fungus	452:457	fungus	452:457	In this study, we isolated a β-glucuronidase (TpGUS79A) from the filamentous fungus Talaromyces pinophilus Li-93 that can specifically and precisely convert GL to GAMG without the formation of the by-product glycyrrhetinic acid (GA) from the further hydrolysis of GAMG.
30054355	12	46	dep	β-glucuronidase	2708:2722	arg1	named					2725:2729	named	2725:2729	named TpGUS79A	2725:2738	In the present study, we succeeded in isolating a novel β-glucuronidase (named TpGUS79A) from Talaromyces pinophilus Li-93 that specifically hydrolyzes GL to GAMG without the formation of GA.
30054355	13	47	theme	characterized	2915:2927	arg1	GUSs					2929:2932	the previously characterized GUSs	2900:2932	the previously characterized GUSs	2900:2932	TpGUS79A also shows higher activity on GL than those of the previously characterized GUSs.
30054355	2	48	theme	GAMG	639:642	arg1	hydrolysis					625:634	the further hydrolysis	613:634	the further hydrolysis of GAMG	613:642	In this study, we isolated a β-glucuronidase (TpGUS79A) from the filamentous fungus Talaromyces pinophilus Li-93 that can specifically and precisely convert GL to GAMG without the formation of the by-product glycyrrhetinic acid (GA) from the further hydrolysis of GAMG.
30054355	5	49	theme	strict	1229:1234	arg1	specificity					1236:1246	strict specificity	1229:1246	strict specificity toward the glycan moiety	1229:1271	TpGUS79A had strict specificity toward the glycan moiety but poor specificity toward the aglycone moiety.
30054355	0	50	theme	Acid	112:115	arg1	3-O-Mono-β-d-Glucuronide					117:140	Glycyrrhetinic Acid 3-O-Mono-β-d-Glucuronide	97:140	Glycyrrhetinic Acid 3-O-Mono-β-d-Glucuronide	97:140	A Novel β-Glucuronidase from Talaromyces pinophilus Li-93 Precisely Hydrolyzes Glycyrrhizin into Glycyrrhetinic Acid 3-O-Mono-β-d-Glucuronide.
30054355	0	51	from	Li-93	52:56	arg1	β-Glucuronidase					8:22	A Novel β-Glucuronidase	0:22	A Novel β-Glucuronidase from Talaromyces pinophilus Li-93	0:56	A Novel β-Glucuronidase from Talaromyces pinophilus Li-93 Precisely Hydrolyzes Glycyrrhizin into Glycyrrhetinic Acid 3-O-Mono-β-d-Glucuronide.
30054355	4	52	theme	GUS	1203:1205	arg1	enzymes					1207:1213	the available GH79 GUS enzymes	1184:1213	the available GH79 GUS enzymes	1184:1213	The gene for TpGUS79A was then cloned and verified by heterologous expression in Pichia pastoris TpGUS79A belonged to glycoside hydrolase family 79 (GH79) but shared low amino acid sequence identity (<35%) with the available GH79 GUS enzymes.
30054355	10	53	contain	has	2220:2222	arg1	3-O-mono-β-d-glucuronide					2181:2204	glycyrrhetinic acid 3-O-mono-β-d-glucuronide	2161:2204	glycyrrhetinic acid 3-O-mono-β-d-glucuronide (GAMG)	2161:2211	Therefore, TpGUS79A is potentially a powerful biocatalyst for environmentally friendly and cost-effective production of GAMG.IMPORTANCE Compared to chemical methods, the biotransformation of glycyrrhizin (GL) into glycyrrhetinic acid 3-O-mono-β-d-glucuronide (GAMG), which has a higher sweetness and stronger pharmacological activity than those of GL, via catalysis by β-glucuronidase is an environmentally friendly approach due to the mild reaction conditions and the high yield of GAMG.
30054355	10	53	contain	has	2220:2222	arg2	activity					2272:2279	stronger pharmacological activity	2247:2279	stronger pharmacological activity	2247:2279	Therefore, TpGUS79A is potentially a powerful biocatalyst for environmentally friendly and cost-effective production of GAMG.IMPORTANCE Compared to chemical methods, the biotransformation of glycyrrhizin (GL) into glycyrrhetinic acid 3-O-mono-β-d-glucuronide (GAMG), which has a higher sweetness and stronger pharmacological activity than those of GL, via catalysis by β-glucuronidase is an environmentally friendly approach due to the mild reaction conditions and the high yield of GAMG.
30054355	10	53	contain	has	2220:2222	arg2	sweetness					2233:2241	a higher sweetness	2224:2241	a higher sweetness	2224:2241	Therefore, TpGUS79A is potentially a powerful biocatalyst for environmentally friendly and cost-effective production of GAMG.IMPORTANCE Compared to chemical methods, the biotransformation of glycyrrhizin (GL) into glycyrrhetinic acid 3-O-mono-β-d-glucuronide (GAMG), which has a higher sweetness and stronger pharmacological activity than those of GL, via catalysis by β-glucuronidase is an environmentally friendly approach due to the mild reaction conditions and the high yield of GAMG.
30054355	10	53	contain	has	2220:2222	arg1	GAMG					2207:2210	GAMG	2207:2210	GAMG	2207:2210	Therefore, TpGUS79A is potentially a powerful biocatalyst for environmentally friendly and cost-effective production of GAMG.IMPORTANCE Compared to chemical methods, the biotransformation of glycyrrhizin (GL) into glycyrrhetinic acid 3-O-mono-β-d-glucuronide (GAMG), which has a higher sweetness and stronger pharmacological activity than those of GL, via catalysis by β-glucuronidase is an environmentally friendly approach due to the mild reaction conditions and the high yield of GAMG.
30054355	7	54	theme	s-1	1625:1627	arg1	kcat/K					1605:1610	kcat/K	1605:1610	kcat/K of 0.01 mM-1 s-1	1605:1627	TpGUS79A showed a much higher catalytic efficiency on GL (kcat/K of 11.14 mM-1 s-1) than on the artificial substrate pNP β-glucopyranosiduronic acid (kcat/K of 0.01 mM-1 s-1), which is different from the case for most GUSs.
30054355	3	55	gly	N-glycosylated	858:871	arg1	TpGUS79A					837:844	TpGUS79A	837:844	TpGUS79A	837:844	First, TpGUS79A was purified and identified through matrix-assisted laser desorption ionization-tandem time of flight mass spectrometry (MALDI-TOF-TOF MS) and deglycosylation, indicating that TpGUS79A is a highly N-glycosylated monomeric protein with a molecular mass of around 85 kDa, including around 25 kDa of glycan moiety.
30054355	3	55	gly	N-glycosylated	858:871	arg1	protein					883:889	a highly N-glycosylated monomeric protein	849:889	a highly N-glycosylated monomeric protein with a molecular mass of around 85 kDa, including around 25 kDa of glycan moiety	849:970	First, TpGUS79A was purified and identified through matrix-assisted laser desorption ionization-tandem time of flight mass spectrometry (MALDI-TOF-TOF MS) and deglycosylation, indicating that TpGUS79A is a highly N-glycosylated monomeric protein with a molecular mass of around 85 kDa, including around 25 kDa of glycan moiety.
30054355	8	56	theme	key	1770:1772	arg1	residues					1774:1781	the key residues	1766:1781	the key residues for substrate recognition	1766:1807	Homology modeling, substrate docking, and sequence alignment were employed to identify the key residues for substrate recognition.
30054355	7	57	theme	0.01	1615:1618	arg1	mM-1					1620:1623	mM-1	1620:1623	mM-1	1620:1623	TpGUS79A showed a much higher catalytic efficiency on GL (kcat/K of 11.14 mM-1 s-1) than on the artificial substrate pNP β-glucopyranosiduronic acid (kcat/K of 0.01 mM-1 s-1), which is different from the case for most GUSs.
30054355	10	58	theme	mild	2383:2386	arg1	conditions					2397:2406	the mild reaction conditions	2379:2406	the mild reaction conditions	2379:2406	Therefore, TpGUS79A is potentially a powerful biocatalyst for environmentally friendly and cost-effective production of GAMG.IMPORTANCE Compared to chemical methods, the biotransformation of glycyrrhizin (GL) into glycyrrhetinic acid 3-O-mono-β-d-glucuronide (GAMG), which has a higher sweetness and stronger pharmacological activity than those of GL, via catalysis by β-glucuronidase is an environmentally friendly approach due to the mild reaction conditions and the high yield of GAMG.
30054355	1	59	from	GL	371:372	arg1	removal					321:327	removal	321:327	removal of the distal glucuronic acid (GlcA) from GL	321:372	Glycyrrhetinic acid 3-O-mono-β-d-glucuronide (GAMG), which possesses a higher sweetness and stronger pharmacological activity than those of glycyrrhizin (GL), can be obtained by removal of the distal glucuronic acid (GlcA) from GL.
30054355	1	60	theme	Glycyrrhetinic	143:156	arg1	acid					158:161	Glycyrrhetinic acid 3-O-mono-β-d-glucuronide	143:186	Glycyrrhetinic acid 3-O-mono-β-d-glucuronide (GAMG)	143:193	Glycyrrhetinic acid 3-O-mono-β-d-glucuronide (GAMG), which possesses a higher sweetness and stronger pharmacological activity than those of glycyrrhizin (GL), can be obtained by removal of the distal glucuronic acid (GlcA) from GL.
30054355	1	60	theme	Glycyrrhetinic	143:156	arg1	GAMG					189:192	GAMG	189:192	GAMG	189:192	Glycyrrhetinic acid 3-O-mono-β-d-glucuronide (GAMG), which possesses a higher sweetness and stronger pharmacological activity than those of glycyrrhizin (GL), can be obtained by removal of the distal glucuronic acid (GlcA) from GL.
30054355	3	61	theme	kDa	926:928	arg1	mass					908:911	a molecular mass	896:911	a molecular mass	896:911	First, TpGUS79A was purified and identified through matrix-assisted laser desorption ionization-tandem time of flight mass spectrometry (MALDI-TOF-TOF MS) and deglycosylation, indicating that TpGUS79A is a highly N-glycosylated monomeric protein with a molecular mass of around 85 kDa, including around 25 kDa of glycan moiety.
30054355	2	62	theme	pinophilus	471:480	arg1	Li-93					482:486	the filamentous fungus Talaromyces pinophilus Li-93	436:486	the filamentous fungus Talaromyces pinophilus Li-93 that can specifically and precisely convert GL to GAMG	436:541	In this study, we isolated a β-glucuronidase (TpGUS79A) from the filamentous fungus Talaromyces pinophilus Li-93 that can specifically and precisely convert GL to GAMG without the formation of the by-product glycyrrhetinic acid (GA) from the further hydrolysis of GAMG.
30054355	0	63	theme	Talaromyces	29:39	arg1	Li-93					52:56	Talaromyces pinophilus Li-93	29:56	Talaromyces pinophilus Li-93	29:56	A Novel β-Glucuronidase from Talaromyces pinophilus Li-93 Precisely Hydrolyzes Glycyrrhizin into Glycyrrhetinic Acid 3-O-Mono-β-d-Glucuronide.
30054355	10	64	theme	high	2416:2419	arg1	yield					2421:2425	the high yield	2412:2425	the high yield of GAMG	2412:2433	Therefore, TpGUS79A is potentially a powerful biocatalyst for environmentally friendly and cost-effective production of GAMG.IMPORTANCE Compared to chemical methods, the biotransformation of glycyrrhizin (GL) into glycyrrhetinic acid 3-O-mono-β-d-glucuronide (GAMG), which has a higher sweetness and stronger pharmacological activity than those of GL, via catalysis by β-glucuronidase is an environmentally friendly approach due to the mild reaction conditions and the high yield of GAMG.
30054355	7	65	theme	catalytic	1485:1493	arg1	efficiency					1495:1504	a much higher catalytic efficiency	1471:1504	a much higher catalytic efficiency on GL (kcat/K of 11.14 mM-1 s-1)	1471:1537	TpGUS79A showed a much higher catalytic efficiency on GL (kcat/K of 11.14 mM-1 s-1) than on the artificial substrate pNP β-glucopyranosiduronic acid (kcat/K of 0.01 mM-1 s-1), which is different from the case for most GUSs.
30054355	6	66	theme	distal	1376:1381	arg1	bond					1394:1397	the distal glucuronic bond	1372:1397	the distal glucuronic bond of GL	1372:1403	Interestingly, TpGUS79A recognized and hydrolyzed the distal glucuronic bond of GL but could not cleave the glucuronic bond in GAMG.
30054355	1	67	theme	distal	336:341	arg1	GlcA					360:363	GlcA	360:363	GlcA	360:363	Glycyrrhetinic acid 3-O-mono-β-d-glucuronide (GAMG), which possesses a higher sweetness and stronger pharmacological activity than those of glycyrrhizin (GL), can be obtained by removal of the distal glucuronic acid (GlcA) from GL.
30054355	1	67	theme	distal	336:341	arg1	acid					354:357	the distal glucuronic acid	332:357	the distal glucuronic acid (GlcA)	332:364	Glycyrrhetinic acid 3-O-mono-β-d-glucuronide (GAMG), which possesses a higher sweetness and stronger pharmacological activity than those of glycyrrhizin (GL), can be obtained by removal of the distal glucuronic acid (GlcA) from GL.
30054355	1	68	theme	acid	354:357	arg1	removal					321:327	removal	321:327	removal of the distal glucuronic acid (GlcA) from GL	321:372	Glycyrrhetinic acid 3-O-mono-β-d-glucuronide (GAMG), which possesses a higher sweetness and stronger pharmacological activity than those of glycyrrhizin (GL), can be obtained by removal of the distal glucuronic acid (GlcA) from GL.
30054355	13	69	theme	higher	2864:2869	arg1	activity					2871:2878	higher activity	2864:2878	higher activity on GL	2864:2884	TpGUS79A also shows higher activity on GL than those of the previously characterized GUSs.
30054355	0	70	dep	Talaromyces	29:39	arg1	pinophilus					41:50	pinophilus	41:50	pinophilus	41:50	A Novel β-Glucuronidase from Talaromyces pinophilus Li-93 Precisely Hydrolyzes Glycyrrhizin into Glycyrrhetinic Acid 3-O-Mono-β-d-Glucuronide.
30054355	10	71	theme	powerful	1984:1991	arg1	biocatalyst					1993:2003	a powerful biocatalyst	1982:2003	a powerful biocatalyst for environmentally friendly and cost-effective production of GAMG.IMPORTANCE Compared to chemical methods	1982:2110	Therefore, TpGUS79A is potentially a powerful biocatalyst for environmentally friendly and cost-effective production of GAMG.IMPORTANCE Compared to chemical methods, the biotransformation of glycyrrhizin (GL) into glycyrrhetinic acid 3-O-mono-β-d-glucuronide (GAMG), which has a higher sweetness and stronger pharmacological activity than those of GL, via catalysis by β-glucuronidase is an environmentally friendly approach due to the mild reaction conditions and the high yield of GAMG.
30054355	10	71	theme	powerful	1984:1991	arg1	TpGUS79A					1958:1965	TpGUS79A	1958:1965	TpGUS79A	1958:1965	Therefore, TpGUS79A is potentially a powerful biocatalyst for environmentally friendly and cost-effective production of GAMG.IMPORTANCE Compared to chemical methods, the biotransformation of glycyrrhizin (GL) into glycyrrhetinic acid 3-O-mono-β-d-glucuronide (GAMG), which has a higher sweetness and stronger pharmacological activity than those of GL, via catalysis by β-glucuronidase is an environmentally friendly approach due to the mild reaction conditions and the high yield of GAMG.
30054355	11	72	theme	glycyrrhetinic	2541:2554	arg1	GA					2562:2563	GA	2562:2563	GA	2562:2563	However, currently available GUSs show low substrate specificity toward GL and further hydrolyze GAMG to glycyrrhetinic acid (GA) as a by-product, increasing the difficulty of subsequent separation and purification.
30054355	11	72	theme	glycyrrhetinic	2541:2554	arg1	acid					2556:2559	glycyrrhetinic acid	2541:2559	glycyrrhetinic acid (GA)	2541:2564	However, currently available GUSs show low substrate specificity toward GL and further hydrolyze GAMG to glycyrrhetinic acid (GA) as a by-product, increasing the difficulty of subsequent separation and purification.
30054355	3	73	theme	moiety	965:970	arg1	kDa					951:953	25 kDa	948:953	25 kDa of glycan moiety	948:970	First, TpGUS79A was purified and identified through matrix-assisted laser desorption ionization-tandem time of flight mass spectrometry (MALDI-TOF-TOF MS) and deglycosylation, indicating that TpGUS79A is a highly N-glycosylated monomeric protein with a molecular mass of around 85 kDa, including around 25 kDa of glycan moiety.
30054355	10	74	theme	cost-effective	2038:2051	arg1	production					2053:2062	cost-effective production	2038:2062	cost-effective production	2038:2062	Therefore, TpGUS79A is potentially a powerful biocatalyst for environmentally friendly and cost-effective production of GAMG.IMPORTANCE Compared to chemical methods, the biotransformation of glycyrrhizin (GL) into glycyrrhetinic acid 3-O-mono-β-d-glucuronide (GAMG), which has a higher sweetness and stronger pharmacological activity than those of GL, via catalysis by β-glucuronidase is an environmentally friendly approach due to the mild reaction conditions and the high yield of GAMG.
30054355	11	75	theme	purification	2638:2649	arg1	difficulty					2598:2607	the difficulty	2594:2607	the difficulty of subsequent separation and purification	2594:2649	However, currently available GUSs show low substrate specificity toward GL and further hydrolyze GAMG to glycyrrhetinic acid (GA) as a by-product, increasing the difficulty of subsequent separation and purification.
30054355	12	76	theme	GA	2840:2841	arg1	formation					2827:2835	the formation	2823:2835	the formation of GA	2823:2841	In the present study, we succeeded in isolating a novel β-glucuronidase (named TpGUS79A) from Talaromyces pinophilus Li-93 that specifically hydrolyzes GL to GAMG without the formation of GA.
30054355	6	77	theme	glucuronic	1430:1439	arg1	bond					1441:1444	the glucuronic bond	1426:1444	the glucuronic bond in GAMG	1426:1452	Interestingly, TpGUS79A recognized and hydrolyzed the distal glucuronic bond of GL but could not cleave the glucuronic bond in GAMG.
30054355	10	78	theme	higher	2226:2231	arg1	sweetness					2233:2241	a higher sweetness	2224:2241	a higher sweetness	2224:2241	Therefore, TpGUS79A is potentially a powerful biocatalyst for environmentally friendly and cost-effective production of GAMG.IMPORTANCE Compared to chemical methods, the biotransformation of glycyrrhizin (GL) into glycyrrhetinic acid 3-O-mono-β-d-glucuronide (GAMG), which has a higher sweetness and stronger pharmacological activity than those of GL, via catalysis by β-glucuronidase is an environmentally friendly approach due to the mild reaction conditions and the high yield of GAMG.
30054355	2	79	from	formation	555:563	arg1	hydrolysis					625:634	the further hydrolysis	613:634	the further hydrolysis of GAMG	613:642	In this study, we isolated a β-glucuronidase (TpGUS79A) from the filamentous fungus Talaromyces pinophilus Li-93 that can specifically and precisely convert GL to GAMG without the formation of the by-product glycyrrhetinic acid (GA) from the further hydrolysis of GAMG.
30054355	9	80	theme	T.	1926:1927	arg1	Li-93					1940:1944	T. pinophilus Li-93	1926:1944	T. pinophilus Li-93	1926:1944	Finally, a fed-batch fermentation in a 150-liter fermentor was established to prepare GAMG through GL hydrolysis by T. pinophilus Li-93.
30054355	7	81	from	efficiency	1495:1504	arg1	GL					1509:1510	GL	1509:1510	GL (kcat/K of 11.14 mM-1 s-1)	1509:1537	TpGUS79A showed a much higher catalytic efficiency on GL (kcat/K of 11.14 mM-1 s-1) than on the artificial substrate pNP β-glucopyranosiduronic acid (kcat/K of 0.01 mM-1 s-1), which is different from the case for most GUSs.
30054355	2	82	theme	glycyrrhetinic	583:596	arg1	GA					604:605	GA	604:605	GA	604:605	In this study, we isolated a β-glucuronidase (TpGUS79A) from the filamentous fungus Talaromyces pinophilus Li-93 that can specifically and precisely convert GL to GAMG without the formation of the by-product glycyrrhetinic acid (GA) from the further hydrolysis of GAMG.
30054355	2	82	theme	glycyrrhetinic	583:596	arg1	acid					598:601	the by-product glycyrrhetinic acid	568:601	the by-product glycyrrhetinic acid (GA) from the further hydrolysis of GAMG	568:642	In this study, we isolated a β-glucuronidase (TpGUS79A) from the filamentous fungus Talaromyces pinophilus Li-93 that can specifically and precisely convert GL to GAMG without the formation of the by-product glycyrrhetinic acid (GA) from the further hydrolysis of GAMG.
30054355	9	83	theme	fed-batch	1821:1829	arg1	fermentation					1831:1842	a fed-batch fermentation	1819:1842	a fed-batch fermentation in a 150-liter fermentor	1819:1867	Finally, a fed-batch fermentation in a 150-liter fermentor was established to prepare GAMG through GL hydrolysis by T. pinophilus Li-93.
30054355	3	84	theme	ionization-tandem	730:746	arg1	time					748:751	matrix-assisted laser desorption ionization-tandem time	697:751	matrix-assisted laser desorption ionization-tandem time of flight mass spectrometry (MALDI-TOF-TOF MS) and deglycosylation	697:818	First, TpGUS79A was purified and identified through matrix-assisted laser desorption ionization-tandem time of flight mass spectrometry (MALDI-TOF-TOF MS) and deglycosylation, indicating that TpGUS79A is a highly N-glycosylated monomeric protein with a molecular mass of around 85 kDa, including around 25 kDa of glycan moiety.
30054355	14	85	dep	pastoris	3037:3044	arg1	serve					3070:3074	serve	3070:3074	can serve as a powerful biocatalyst for the cost-effective production of GAMG through GL transformation	3066:3168	Moreover, the gene for TpGUS79A was cloned and its function verified by heterologous expression in P. pastoris Therefore, TpGUS79A can serve as a powerful biocatalyst for the cost-effective production of GAMG through GL transformation.
30054355	10	86	theme	pharmacological	2256:2270	arg1	activity					2272:2279	stronger pharmacological activity	2247:2279	stronger pharmacological activity	2247:2279	Therefore, TpGUS79A is potentially a powerful biocatalyst for environmentally friendly and cost-effective production of GAMG.IMPORTANCE Compared to chemical methods, the biotransformation of glycyrrhizin (GL) into glycyrrhetinic acid 3-O-mono-β-d-glucuronide (GAMG), which has a higher sweetness and stronger pharmacological activity than those of GL, via catalysis by β-glucuronidase is an environmentally friendly approach due to the mild reaction conditions and the high yield of GAMG.
30054355	3	87	theme	laser	713:717	arg1	time					748:751	matrix-assisted laser desorption ionization-tandem time	697:751	matrix-assisted laser desorption ionization-tandem time of flight mass spectrometry (MALDI-TOF-TOF MS) and deglycosylation	697:818	First, TpGUS79A was purified and identified through matrix-assisted laser desorption ionization-tandem time of flight mass spectrometry (MALDI-TOF-TOF MS) and deglycosylation, indicating that TpGUS79A is a highly N-glycosylated monomeric protein with a molecular mass of around 85 kDa, including around 25 kDa of glycan moiety.
30054355	4	88	theme	hydrolase	1101:1109	arg1	family					1111:1116	glycoside hydrolase family 79	1091:1119	glycoside hydrolase family 79 (GH79)	1091:1126	The gene for TpGUS79A was then cloned and verified by heterologous expression in Pichia pastoris TpGUS79A belonged to glycoside hydrolase family 79 (GH79) but shared low amino acid sequence identity (<35%) with the available GH79 GUS enzymes.
30054355	4	88	theme	hydrolase	1101:1109	arg1	GH79					1122:1125	GH79	1122:1125	GH79	1122:1125	The gene for TpGUS79A was then cloned and verified by heterologous expression in Pichia pastoris TpGUS79A belonged to glycoside hydrolase family 79 (GH79) but shared low amino acid sequence identity (<35%) with the available GH79 GUS enzymes.
30054355	3	89	theme	mass	763:766	arg1	spectrometry					768:779	flight mass spectrometry	756:779	flight mass spectrometry (MALDI-TOF-TOF MS)	756:798	First, TpGUS79A was purified and identified through matrix-assisted laser desorption ionization-tandem time of flight mass spectrometry (MALDI-TOF-TOF MS) and deglycosylation, indicating that TpGUS79A is a highly N-glycosylated monomeric protein with a molecular mass of around 85 kDa, including around 25 kDa of glycan moiety.
30054355	3	89	theme	mass	763:766	arg1	MS					796:797	MALDI-TOF-TOF MS	782:797	MALDI-TOF-TOF MS	782:797	First, TpGUS79A was purified and identified through matrix-assisted laser desorption ionization-tandem time of flight mass spectrometry (MALDI-TOF-TOF MS) and deglycosylation, indicating that TpGUS79A is a highly N-glycosylated monomeric protein with a molecular mass of around 85 kDa, including around 25 kDa of glycan moiety.
30054355	10	90	theme	glycyrrhetinic	2161:2174	arg1	acid					2176:2179	glycyrrhetinic acid	2161:2179	glycyrrhetinic acid 3-O-mono-β-d-glucuronide (GAMG)	2161:2211	Therefore, TpGUS79A is potentially a powerful biocatalyst for environmentally friendly and cost-effective production of GAMG.IMPORTANCE Compared to chemical methods, the biotransformation of glycyrrhizin (GL) into glycyrrhetinic acid 3-O-mono-β-d-glucuronide (GAMG), which has a higher sweetness and stronger pharmacological activity than those of GL, via catalysis by β-glucuronidase is an environmentally friendly approach due to the mild reaction conditions and the high yield of GAMG.
30054355	9	91	theme	pinophilus	1929:1938	arg1	Li-93					1940:1944	T. pinophilus Li-93	1926:1944	T. pinophilus Li-93	1926:1944	Finally, a fed-batch fermentation in a 150-liter fermentor was established to prepare GAMG through GL hydrolysis by T. pinophilus Li-93.
30054355	1	92	theme	stronger	235:242	arg1	activity					260:267	stronger pharmacological activity	235:267	stronger pharmacological activity	235:267	Glycyrrhetinic acid 3-O-mono-β-d-glucuronide (GAMG), which possesses a higher sweetness and stronger pharmacological activity than those of glycyrrhizin (GL), can be obtained by removal of the distal glucuronic acid (GlcA) from GL.
30054355	7	93	theme	pNP	1572:1574	arg1	acid					1599:1602	the artificial substrate pNP β-glucopyranosiduronic acid	1547:1602	the artificial substrate pNP β-glucopyranosiduronic acid (kcat/K of 0.01 mM-1 s-1)	1547:1628	TpGUS79A showed a much higher catalytic efficiency on GL (kcat/K of 11.14 mM-1 s-1) than on the artificial substrate pNP β-glucopyranosiduronic acid (kcat/K of 0.01 mM-1 s-1), which is different from the case for most GUSs.
30054355	7	93	theme	pNP	1572:1574	arg1	different					1640:1648	different	1640:1648	different	1640:1648	TpGUS79A showed a much higher catalytic efficiency on GL (kcat/K of 11.14 mM-1 s-1) than on the artificial substrate pNP β-glucopyranosiduronic acid (kcat/K of 0.01 mM-1 s-1), which is different from the case for most GUSs.
30054355	11	94	theme	substrate	2479:2487	arg1	specificity					2489:2499	low substrate specificity	2475:2499	low substrate specificity toward GL	2475:2509	However, currently available GUSs show low substrate specificity toward GL and further hydrolyze GAMG to glycyrrhetinic acid (GA) as a by-product, increasing the difficulty of subsequent separation and purification.
30054355	10	95	theme	chemical	2095:2102	arg1	methods					2104:2110	chemical methods	2095:2110	chemical methods	2095:2110	Therefore, TpGUS79A is potentially a powerful biocatalyst for environmentally friendly and cost-effective production of GAMG.IMPORTANCE Compared to chemical methods, the biotransformation of glycyrrhizin (GL) into glycyrrhetinic acid 3-O-mono-β-d-glucuronide (GAMG), which has a higher sweetness and stronger pharmacological activity than those of GL, via catalysis by β-glucuronidase is an environmentally friendly approach due to the mild reaction conditions and the high yield of GAMG.
30054355	9	96	theme	150-liter	1849:1857	arg1	fermentor					1859:1867	a 150-liter fermentor	1847:1867	a 150-liter fermentor	1847:1867	Finally, a fed-batch fermentation in a 150-liter fermentor was established to prepare GAMG through GL hydrolysis by T. pinophilus Li-93.
30054355	7	97	theme	artificial	1551:1560	arg1	acid					1599:1602	the artificial substrate pNP β-glucopyranosiduronic acid	1547:1602	the artificial substrate pNP β-glucopyranosiduronic acid (kcat/K of 0.01 mM-1 s-1)	1547:1628	TpGUS79A showed a much higher catalytic efficiency on GL (kcat/K of 11.14 mM-1 s-1) than on the artificial substrate pNP β-glucopyranosiduronic acid (kcat/K of 0.01 mM-1 s-1), which is different from the case for most GUSs.
30054355	7	97	theme	artificial	1551:1560	arg1	different					1640:1648	different	1640:1648	different	1640:1648	TpGUS79A showed a much higher catalytic efficiency on GL (kcat/K of 11.14 mM-1 s-1) than on the artificial substrate pNP β-glucopyranosiduronic acid (kcat/K of 0.01 mM-1 s-1), which is different from the case for most GUSs.
30054355	10	98	theme	glycyrrhizin	2138:2149	arg1	approach					2363:2370	an environmentally friendly approach	2335:2370	an environmentally friendly approach due to the mild reaction conditions and the high yield of GAMG	2335:2433	Therefore, TpGUS79A is potentially a powerful biocatalyst for environmentally friendly and cost-effective production of GAMG.IMPORTANCE Compared to chemical methods, the biotransformation of glycyrrhizin (GL) into glycyrrhetinic acid 3-O-mono-β-d-glucuronide (GAMG), which has a higher sweetness and stronger pharmacological activity than those of GL, via catalysis by β-glucuronidase is an environmentally friendly approach due to the mild reaction conditions and the high yield of GAMG.
30054355	10	98	theme	glycyrrhizin	2138:2149	arg1	biotransformation					2117:2133	the biotransformation	2113:2133	the biotransformation of glycyrrhizin (GL) into glycyrrhetinic acid 3-O-mono-β-d-glucuronide (GAMG), which has a higher sweetness and stronger pharmacological activity than those of GL, via catalysis by β-glucuronidase	2113:2330	Therefore, TpGUS79A is potentially a powerful biocatalyst for environmentally friendly and cost-effective production of GAMG.IMPORTANCE Compared to chemical methods, the biotransformation of glycyrrhizin (GL) into glycyrrhetinic acid 3-O-mono-β-d-glucuronide (GAMG), which has a higher sweetness and stronger pharmacological activity than those of GL, via catalysis by β-glucuronidase is an environmentally friendly approach due to the mild reaction conditions and the high yield of GAMG.
30054355	2	99	from	hydrolysis	625:634	arg1	GA					604:605	GA	604:605	GA	604:605	In this study, we isolated a β-glucuronidase (TpGUS79A) from the filamentous fungus Talaromyces pinophilus Li-93 that can specifically and precisely convert GL to GAMG without the formation of the by-product glycyrrhetinic acid (GA) from the further hydrolysis of GAMG.
30054355	2	99	from	hydrolysis	625:634	arg1	acid					598:601	the by-product glycyrrhetinic acid	568:601	the by-product glycyrrhetinic acid (GA) from the further hydrolysis of GAMG	568:642	In this study, we isolated a β-glucuronidase (TpGUS79A) from the filamentous fungus Talaromyces pinophilus Li-93 that can specifically and precisely convert GL to GAMG without the formation of the by-product glycyrrhetinic acid (GA) from the further hydrolysis of GAMG.
30054355	2	99	from	hydrolysis	625:634	arg1	formation					555:563	the formation	551:563	the formation of the by-product glycyrrhetinic acid (GA) from the further hydrolysis of GAMG	551:642	In this study, we isolated a β-glucuronidase (TpGUS79A) from the filamentous fungus Talaromyces pinophilus Li-93 that can specifically and precisely convert GL to GAMG without the formation of the by-product glycyrrhetinic acid (GA) from the further hydrolysis of GAMG.
30054355	4	100	theme	available	1188:1196	arg1	enzymes					1207:1213	the available GH79 GUS enzymes	1184:1213	the available GH79 GUS enzymes	1184:1213	The gene for TpGUS79A was then cloned and verified by heterologous expression in Pichia pastoris TpGUS79A belonged to glycoside hydrolase family 79 (GH79) but shared low amino acid sequence identity (<35%) with the available GH79 GUS enzymes.
30054355	3	101	theme	monomeric	873:881	arg1	TpGUS79A					837:844	TpGUS79A	837:844	TpGUS79A	837:844	First, TpGUS79A was purified and identified through matrix-assisted laser desorption ionization-tandem time of flight mass spectrometry (MALDI-TOF-TOF MS) and deglycosylation, indicating that TpGUS79A is a highly N-glycosylated monomeric protein with a molecular mass of around 85 kDa, including around 25 kDa of glycan moiety.
30054355	3	101	theme	monomeric	873:881	arg1	protein					883:889	a highly N-glycosylated monomeric protein	849:889	a highly N-glycosylated monomeric protein with a molecular mass of around 85 kDa, including around 25 kDa of glycan moiety	849:970	First, TpGUS79A was purified and identified through matrix-assisted laser desorption ionization-tandem time of flight mass spectrometry (MALDI-TOF-TOF MS) and deglycosylation, indicating that TpGUS79A is a highly N-glycosylated monomeric protein with a molecular mass of around 85 kDa, including around 25 kDa of glycan moiety.
30054355	4	102	theme	low	1139:1141	arg1	identity					1163:1170	low amino acid sequence identity	1139:1170	low amino acid sequence identity (<35%) with the available GH79 GUS enzymes	1139:1213	The gene for TpGUS79A was then cloned and verified by heterologous expression in Pichia pastoris TpGUS79A belonged to glycoside hydrolase family 79 (GH79) but shared low amino acid sequence identity (<35%) with the available GH79 GUS enzymes.
30054355	4	102	theme	low	1139:1141	arg1	%					1176:1176	<35%	1173:1176	<35%	1173:1176	The gene for TpGUS79A was then cloned and verified by heterologous expression in Pichia pastoris TpGUS79A belonged to glycoside hydrolase family 79 (GH79) but shared low amino acid sequence identity (<35%) with the available GH79 GUS enzymes.
30054355	11	103	theme	separation	2623:2632	arg1	difficulty					2598:2607	the difficulty	2594:2607	the difficulty of subsequent separation and purification	2594:2649	However, currently available GUSs show low substrate specificity toward GL and further hydrolyze GAMG to glycyrrhetinic acid (GA) as a by-product, increasing the difficulty of subsequent separation and purification.
30054355	8	104	theme	substrate	1698:1706	arg1	docking					1708:1714	substrate docking	1698:1714	substrate docking	1698:1714	Homology modeling, substrate docking, and sequence alignment were employed to identify the key residues for substrate recognition.
30054355	7	105	theme	mM-1	1529:1532	arg1	s-1					1534:1536	11.14 mM-1 s-1	1523:1536	11.14 mM-1 s-1	1523:1536	TpGUS79A showed a much higher catalytic efficiency on GL (kcat/K of 11.14 mM-1 s-1) than on the artificial substrate pNP β-glucopyranosiduronic acid (kcat/K of 0.01 mM-1 s-1), which is different from the case for most GUSs.
30054355	9	106	from	fermentation	1831:1842	arg1	fermentor					1859:1867	a 150-liter fermentor	1847:1867	a 150-liter fermentor	1847:1867	Finally, a fed-batch fermentation in a 150-liter fermentor was established to prepare GAMG through GL hydrolysis by T. pinophilus Li-93.
30054355	4	107	theme	acid	1149:1152	arg1	identity					1163:1170	low amino acid sequence identity	1139:1170	low amino acid sequence identity (<35%) with the available GH79 GUS enzymes	1139:1213	The gene for TpGUS79A was then cloned and verified by heterologous expression in Pichia pastoris TpGUS79A belonged to glycoside hydrolase family 79 (GH79) but shared low amino acid sequence identity (<35%) with the available GH79 GUS enzymes.
30054355	4	107	theme	acid	1149:1152	arg1	%					1176:1176	<35%	1173:1176	<35%	1173:1176	The gene for TpGUS79A was then cloned and verified by heterologous expression in Pichia pastoris TpGUS79A belonged to glycoside hydrolase family 79 (GH79) but shared low amino acid sequence identity (<35%) with the available GH79 GUS enzymes.
30054355	0	108	theme	Glycyrrhetinic	97:110	arg1	Acid					112:115	Glycyrrhetinic Acid	97:115	Glycyrrhetinic Acid 3-O-Mono-β-d-Glucuronide	97:140	A Novel β-Glucuronidase from Talaromyces pinophilus Li-93 Precisely Hydrolyzes Glycyrrhizin into Glycyrrhetinic Acid 3-O-Mono-β-d-Glucuronide.
30054355	4	109	from	expression	1040:1049	arg1	TpGUS79A					1070:1077	TpGUS79A	1070:1077	TpGUS79A	1070:1077	The gene for TpGUS79A was then cloned and verified by heterologous expression in Pichia pastoris TpGUS79A belonged to glycoside hydrolase family 79 (GH79) but shared low amino acid sequence identity (<35%) with the available GH79 GUS enzymes.
30054355	4	109	from	expression	1040:1049	arg1	pastoris					1061:1068	Pichia pastoris	1054:1068	Pichia pastoris TpGUS79A belonged to glycoside hydrolase family 79 (GH79) but shared low amino acid sequence identity (<35%) with the available GH79 GUS enzymes	1054:1213	The gene for TpGUS79A was then cloned and verified by heterologous expression in Pichia pastoris TpGUS79A belonged to glycoside hydrolase family 79 (GH79) but shared low amino acid sequence identity (<35%) with the available GH79 GUS enzymes.
30054355	3	110	theme	molecular	898:906	arg1	mass					908:911	a molecular mass	896:911	a molecular mass	896:911	First, TpGUS79A was purified and identified through matrix-assisted laser desorption ionization-tandem time of flight mass spectrometry (MALDI-TOF-TOF MS) and deglycosylation, indicating that TpGUS79A is a highly N-glycosylated monomeric protein with a molecular mass of around 85 kDa, including around 25 kDa of glycan moiety.
30054355	4	111	theme	GH79	1198:1201	arg1	enzymes					1207:1213	the available GH79 GUS enzymes	1184:1213	the available GH79 GUS enzymes	1184:1213	The gene for TpGUS79A was then cloned and verified by heterologous expression in Pichia pastoris TpGUS79A belonged to glycoside hydrolase family 79 (GH79) but shared low amino acid sequence identity (<35%) with the available GH79 GUS enzymes.
30054355	8	112	theme	sequence	1721:1728	arg1	alignment					1730:1738	sequence alignment	1721:1738	sequence alignment	1721:1738	Homology modeling, substrate docking, and sequence alignment were employed to identify the key residues for substrate recognition.
30054355	11	113	theme	available	2455:2463	arg1	GUSs					2465:2468	currently available GUSs	2445:2468	currently available GUSs	2445:2468	However, currently available GUSs show low substrate specificity toward GL and further hydrolyze GAMG to glycyrrhetinic acid (GA) as a by-product, increasing the difficulty of subsequent separation and purification.
30054355	10	114	theme	acid	2176:2179	arg1	3-O-mono-β-d-glucuronide					2181:2204	glycyrrhetinic acid 3-O-mono-β-d-glucuronide	2161:2204	glycyrrhetinic acid 3-O-mono-β-d-glucuronide (GAMG)	2161:2211	Therefore, TpGUS79A is potentially a powerful biocatalyst for environmentally friendly and cost-effective production of GAMG.IMPORTANCE Compared to chemical methods, the biotransformation of glycyrrhizin (GL) into glycyrrhetinic acid 3-O-mono-β-d-glucuronide (GAMG), which has a higher sweetness and stronger pharmacological activity than those of GL, via catalysis by β-glucuronidase is an environmentally friendly approach due to the mild reaction conditions and the high yield of GAMG.
30054355	10	114	theme	acid	2176:2179	arg1	GAMG					2207:2210	GAMG	2207:2210	GAMG	2207:2210	Therefore, TpGUS79A is potentially a powerful biocatalyst for environmentally friendly and cost-effective production of GAMG.IMPORTANCE Compared to chemical methods, the biotransformation of glycyrrhizin (GL) into glycyrrhetinic acid 3-O-mono-β-d-glucuronide (GAMG), which has a higher sweetness and stronger pharmacological activity than those of GL, via catalysis by β-glucuronidase is an environmentally friendly approach due to the mild reaction conditions and the high yield of GAMG.
30054355	14	115	theme	GAMG	3139:3142	arg1	production					3125:3134	the cost-effective production	3106:3134	the cost-effective production of GAMG through GL transformation	3106:3168	Moreover, the gene for TpGUS79A was cloned and its function verified by heterologous expression in P. pastoris Therefore, TpGUS79A can serve as a powerful biocatalyst for the cost-effective production of GAMG through GL transformation.
30054355	10	116	theme	friendly	2354:2361	arg1	approach					2363:2370	an environmentally friendly approach	2335:2370	an environmentally friendly approach due to the mild reaction conditions and the high yield of GAMG	2335:2433	Therefore, TpGUS79A is potentially a powerful biocatalyst for environmentally friendly and cost-effective production of GAMG.IMPORTANCE Compared to chemical methods, the biotransformation of glycyrrhizin (GL) into glycyrrhetinic acid 3-O-mono-β-d-glucuronide (GAMG), which has a higher sweetness and stronger pharmacological activity than those of GL, via catalysis by β-glucuronidase is an environmentally friendly approach due to the mild reaction conditions and the high yield of GAMG.
30054355	10	116	theme	friendly	2354:2361	arg1	biotransformation					2117:2133	the biotransformation	2113:2133	the biotransformation of glycyrrhizin (GL) into glycyrrhetinic acid 3-O-mono-β-d-glucuronide (GAMG), which has a higher sweetness and stronger pharmacological activity than those of GL, via catalysis by β-glucuronidase	2113:2330	Therefore, TpGUS79A is potentially a powerful biocatalyst for environmentally friendly and cost-effective production of GAMG.IMPORTANCE Compared to chemical methods, the biotransformation of glycyrrhizin (GL) into glycyrrhetinic acid 3-O-mono-β-d-glucuronide (GAMG), which has a higher sweetness and stronger pharmacological activity than those of GL, via catalysis by β-glucuronidase is an environmentally friendly approach due to the mild reaction conditions and the high yield of GAMG.
30054355	5	117	theme	aglycone	1305:1312	arg1	moiety					1314:1319	the aglycone moiety	1301:1319	the aglycone moiety	1301:1319	TpGUS79A had strict specificity toward the glycan moiety but poor specificity toward the aglycone moiety.
30054355	0	118	theme	Novel	2:6	arg1	β-Glucuronidase					8:22	A Novel β-Glucuronidase	0:22	A Novel β-Glucuronidase from Talaromyces pinophilus Li-93	0:56	A Novel β-Glucuronidase from Talaromyces pinophilus Li-93 Precisely Hydrolyzes Glycyrrhizin into Glycyrrhetinic Acid 3-O-Mono-β-d-Glucuronide.
30054355	10	119	theme	reaction	2388:2395	arg1	conditions					2397:2406	the mild reaction conditions	2379:2406	the mild reaction conditions	2379:2406	Therefore, TpGUS79A is potentially a powerful biocatalyst for environmentally friendly and cost-effective production of GAMG.IMPORTANCE Compared to chemical methods, the biotransformation of glycyrrhizin (GL) into glycyrrhetinic acid 3-O-mono-β-d-glucuronide (GAMG), which has a higher sweetness and stronger pharmacological activity than those of GL, via catalysis by β-glucuronidase is an environmentally friendly approach due to the mild reaction conditions and the high yield of GAMG.
30054355	4	120	dep	pastoris	1061:1068	arg1	shared					1132:1137	shared	1132:1137	shared low amino acid sequence identity (<35%) with the available GH79 GUS enzymes	1132:1213	The gene for TpGUS79A was then cloned and verified by heterologous expression in Pichia pastoris TpGUS79A belonged to glycoside hydrolase family 79 (GH79) but shared low amino acid sequence identity (<35%) with the available GH79 GUS enzymes.
30054355	4	120	dep	pastoris	1061:1068	arg1	belonged					1079:1086	belonged	1079:1086	belonged to glycoside hydrolase family 79 (GH79)	1079:1126	The gene for TpGUS79A was then cloned and verified by heterologous expression in Pichia pastoris TpGUS79A belonged to glycoside hydrolase family 79 (GH79) but shared low amino acid sequence identity (<35%) with the available GH79 GUS enzymes.
30054355	7	121	theme	mM-1	1620:1623	arg1	s-1					1625:1627	0.01 mM-1 s-1	1615:1627	0.01 mM-1 s-1	1615:1627	TpGUS79A showed a much higher catalytic efficiency on GL (kcat/K of 11.14 mM-1 s-1) than on the artificial substrate pNP β-glucopyranosiduronic acid (kcat/K of 0.01 mM-1 s-1), which is different from the case for most GUSs.
30054355	5	122	theme	poor	1277:1280	arg1	specificity					1282:1292	poor specificity	1277:1292	poor specificity toward the aglycone moiety	1277:1319	TpGUS79A had strict specificity toward the glycan moiety but poor specificity toward the aglycone moiety.
30054355	2	123	theme	Talaromyces	459:469	arg1	Li-93					482:486	the filamentous fungus Talaromyces pinophilus Li-93	436:486	the filamentous fungus Talaromyces pinophilus Li-93 that can specifically and precisely convert GL to GAMG	436:541	In this study, we isolated a β-glucuronidase (TpGUS79A) from the filamentous fungus Talaromyces pinophilus Li-93 that can specifically and precisely convert GL to GAMG without the formation of the by-product glycyrrhetinic acid (GA) from the further hydrolysis of GAMG.
30054355	14	124	theme	GL	3152:3153	arg1	transformation					3155:3168	GL transformation	3152:3168	GL transformation	3152:3168	Moreover, the gene for TpGUS79A was cloned and its function verified by heterologous expression in P. pastoris Therefore, TpGUS79A can serve as a powerful biocatalyst for the cost-effective production of GAMG through GL transformation.
30054355	6	125	theme	glucuronic	1383:1392	arg1	bond					1394:1397	the distal glucuronic bond	1372:1397	the distal glucuronic bond of GL	1372:1403	Interestingly, TpGUS79A recognized and hydrolyzed the distal glucuronic bond of GL but could not cleave the glucuronic bond in GAMG.
30054355	3	126	theme	glycan	958:963	arg1	moiety					965:970	glycan moiety	958:970	glycan moiety	958:970	First, TpGUS79A was purified and identified through matrix-assisted laser desorption ionization-tandem time of flight mass spectrometry (MALDI-TOF-TOF MS) and deglycosylation, indicating that TpGUS79A is a highly N-glycosylated monomeric protein with a molecular mass of around 85 kDa, including around 25 kDa of glycan moiety.
30054355	2	127	theme	filamentous	440:450	arg1	Li-93					482:486	the filamentous fungus Talaromyces pinophilus Li-93	436:486	the filamentous fungus Talaromyces pinophilus Li-93 that can specifically and precisely convert GL to GAMG	436:541	In this study, we isolated a β-glucuronidase (TpGUS79A) from the filamentous fungus Talaromyces pinophilus Li-93 that can specifically and precisely convert GL to GAMG without the formation of the by-product glycyrrhetinic acid (GA) from the further hydrolysis of GAMG.
30054355	1	128	theme	glucuronic	343:352	arg1	GlcA					360:363	GlcA	360:363	GlcA	360:363	Glycyrrhetinic acid 3-O-mono-β-d-glucuronide (GAMG), which possesses a higher sweetness and stronger pharmacological activity than those of glycyrrhizin (GL), can be obtained by removal of the distal glucuronic acid (GlcA) from GL.
30054355	1	128	theme	glucuronic	343:352	arg1	acid					354:357	the distal glucuronic acid	332:357	the distal glucuronic acid (GlcA)	332:364	Glycyrrhetinic acid 3-O-mono-β-d-glucuronide (GAMG), which possesses a higher sweetness and stronger pharmacological activity than those of glycyrrhizin (GL), can be obtained by removal of the distal glucuronic acid (GlcA) from GL.
30054355	7	129	theme	higher	1478:1483	arg1	efficiency					1495:1504	a much higher catalytic efficiency	1471:1504	a much higher catalytic efficiency on GL (kcat/K of 11.14 mM-1 s-1)	1471:1537	TpGUS79A showed a much higher catalytic efficiency on GL (kcat/K of 11.14 mM-1 s-1) than on the artificial substrate pNP β-glucopyranosiduronic acid (kcat/K of 0.01 mM-1 s-1), which is different from the case for most GUSs.
30054355	14	130	theme	powerful	3081:3088	arg1	TpGUS79A					3057:3064	TpGUS79A	3057:3064	TpGUS79A	3057:3064	Moreover, the gene for TpGUS79A was cloned and its function verified by heterologous expression in P. pastoris Therefore, TpGUS79A can serve as a powerful biocatalyst for the cost-effective production of GAMG through GL transformation.
30054355	14	130	theme	powerful	3081:3088	arg1	biocatalyst					3090:3100	a powerful biocatalyst	3079:3100	a powerful biocatalyst for the cost-effective production of GAMG through GL transformation	3079:3168	Moreover, the gene for TpGUS79A was cloned and its function verified by heterologous expression in P. pastoris Therefore, TpGUS79A can serve as a powerful biocatalyst for the cost-effective production of GAMG through GL transformation.
30054355	6	131	from	bond	1441:1444	arg1	GAMG					1449:1452	GAMG	1449:1452	GAMG	1449:1452	Interestingly, TpGUS79A recognized and hydrolyzed the distal glucuronic bond of GL but could not cleave the glucuronic bond in GAMG.
30054355	7	132	dep	GL	1509:1510	arg1	kcat/K					1513:1518	kcat/K	1513:1518	kcat/K of 11.14 mM-1 s-1	1513:1536	TpGUS79A showed a much higher catalytic efficiency on GL (kcat/K of 11.14 mM-1 s-1) than on the artificial substrate pNP β-glucopyranosiduronic acid (kcat/K of 0.01 mM-1 s-1), which is different from the case for most GUSs.
33654919	2	0	theme	muropeptides	346:357	arg1	abundance					333:341	the abundance	329:341	the abundance of muropeptides using high-pressure liquid chromatography	329:399	Cell wall composition can be best characterized at the molecular level by digesting the peptidoglycan murein polymer into its muropeptide subunits and quantifying the abundance of muropeptides using high-pressure liquid chromatography.
33654919	6	1	theme	HPLC	1083:1086	arg1	analysis					1088:1095	HPLC analysis	1083:1095	HPLC analysis	1083:1095	Although the methods for peptidoglycan purification and HPLC analysis have been previously published, our method includes important details on how to re-equilibrate the column between runs to allow for automated analysis of multiple samples.
33654919	1	2	theme	bacterial	144:152	arg1	shape					159:163	bacterial cell shape	144:163	bacterial cell shape	144:163	The peptidoglycan sacculus, or cell wall, is what defines bacterial cell shape.
33654919	3	3	theme	peptidoglycan	548:560	arg1	modifications					562:574	other peptidoglycan modifications	542:574	other peptidoglycan modifications	542:574	Certain features of the cell wall including muropeptide composition, glycan strand length, degree of crosslinking, type of crosslinking and other peptidoglycan modifications can be quantified using this approach.
33654919	4	4	theme	Well-established	615:630	arg1	protocols					632:640	Well-established protocols	615:640	Well-established protocols	615:640	Well-established protocols provide us with highly-resolved and quantitatively reproducible chromatographic data, which can be used to investigate bacterial cell wall composition under a variety of environmental or genetic perturbations.
33654919	6	5	theme	automated	1229:1237	arg1	analysis					1239:1246	automated analysis	1229:1246	automated analysis of multiple samples	1229:1266	Although the methods for peptidoglycan purification and HPLC analysis have been previously published, our method includes important details on how to re-equilibrate the column between runs to allow for automated analysis of multiple samples.
33654919	6	6	theme	samples	1260:1266	arg1	analysis					1239:1246	automated analysis	1229:1246	automated analysis of multiple samples	1229:1266	Although the methods for peptidoglycan purification and HPLC analysis have been previously published, our method includes important details on how to re-equilibrate the column between runs to allow for automated analysis of multiple samples.
33654919	2	7	theme	murein	268:273	arg1	polymer					275:281	the peptidoglycan murein polymer	250:281	the peptidoglycan murein polymer into its muropeptide subunits	250:311	Cell wall composition can be best characterized at the molecular level by digesting the peptidoglycan murein polymer into its muropeptide subunits and quantifying the abundance of muropeptides using high-pressure liquid chromatography.
33654919	6	8	theme	peptidoglycan	1052:1064	arg1	purification					1066:1077	peptidoglycan purification	1052:1077	peptidoglycan purification	1052:1077	Although the methods for peptidoglycan purification and HPLC analysis have been previously published, our method includes important details on how to re-equilibrate the column between runs to allow for automated analysis of multiple samples.
33654919	5	9	theme	mass	1008:1011	arg1	spectrometry					1013:1024	mass spectrometry	1008:1024	mass spectrometry	1008:1024	The method described here enables the purification of muropeptide samples, their quantification by HPLC, and fraction collection for peak identification by mass spectrometry.
33654919	2	10	theme	peptidoglycan	254:266	arg1	polymer					275:281	the peptidoglycan murein polymer	250:281	the peptidoglycan murein polymer into its muropeptide subunits	250:311	Cell wall composition can be best characterized at the molecular level by digesting the peptidoglycan murein polymer into its muropeptide subunits and quantifying the abundance of muropeptides using high-pressure liquid chromatography.
33654919	6	11	theme	important	1149:1157	arg1	details					1159:1165	important details	1149:1165	important details on how to re-equilibrate the column between runs to allow for automated analysis of multiple samples	1149:1266	Although the methods for peptidoglycan purification and HPLC analysis have been previously published, our method includes important details on how to re-equilibrate the column between runs to allow for automated analysis of multiple samples.
33654919	1	12	theme	cell	154:157	arg1	shape					159:163	bacterial cell shape	144:163	bacterial cell shape	144:163	The peptidoglycan sacculus, or cell wall, is what defines bacterial cell shape.
33654919	4	13	used	used	741:744	arg2	data					722:725	highly-resolved and quantitatively reproducible chromatographic data	658:725	highly-resolved and quantitatively reproducible chromatographic data	658:725	Well-established protocols provide us with highly-resolved and quantitatively reproducible chromatographic data, which can be used to investigate bacterial cell wall composition under a variety of environmental or genetic perturbations.
33654919	3	14	theme	other	542:546	arg1	modifications					562:574	other peptidoglycan modifications	542:574	other peptidoglycan modifications	542:574	Certain features of the cell wall including muropeptide composition, glycan strand length, degree of crosslinking, type of crosslinking and other peptidoglycan modifications can be quantified using this approach.
33654919	4	15	theme	chromatographic	706:720	arg1	data					722:725	highly-resolved and quantitatively reproducible chromatographic data	658:725	highly-resolved and quantitatively reproducible chromatographic data	658:725	Well-established protocols provide us with highly-resolved and quantitatively reproducible chromatographic data, which can be used to investigate bacterial cell wall composition under a variety of environmental or genetic perturbations.
33654919	3	16	theme	wall	431:434	arg1	type					517:520	type	517:520	type of crosslinking	517:536	Certain features of the cell wall including muropeptide composition, glycan strand length, degree of crosslinking, type of crosslinking and other peptidoglycan modifications can be quantified using this approach.
33654919	3	16	theme	wall	431:434	arg1	composition					458:468	muropeptide composition	446:468	muropeptide composition	446:468	Certain features of the cell wall including muropeptide composition, glycan strand length, degree of crosslinking, type of crosslinking and other peptidoglycan modifications can be quantified using this approach.
33654919	3	16	theme	wall	431:434	arg1	degree					493:498	degree	493:498	degree of crosslinking	493:514	Certain features of the cell wall including muropeptide composition, glycan strand length, degree of crosslinking, type of crosslinking and other peptidoglycan modifications can be quantified using this approach.
33654919	3	16	theme	wall	431:434	arg1	features					410:417	Certain features	402:417	Certain features of the cell wall including muropeptide composition, glycan strand length, degree of crosslinking, type of crosslinking and other peptidoglycan modifications	402:574	Certain features of the cell wall including muropeptide composition, glycan strand length, degree of crosslinking, type of crosslinking and other peptidoglycan modifications can be quantified using this approach.
33654919	3	16	theme	wall	431:434	arg1	modifications					562:574	other peptidoglycan modifications	542:574	other peptidoglycan modifications	542:574	Certain features of the cell wall including muropeptide composition, glycan strand length, degree of crosslinking, type of crosslinking and other peptidoglycan modifications can be quantified using this approach.
33654919	3	16	theme	wall	431:434	arg1	length					485:490	glycan strand length	471:490	glycan strand length	471:490	Certain features of the cell wall including muropeptide composition, glycan strand length, degree of crosslinking, type of crosslinking and other peptidoglycan modifications can be quantified using this approach.
33654919	3	17	theme	Certain	402:408	arg1	type					517:520	type	517:520	type of crosslinking	517:536	Certain features of the cell wall including muropeptide composition, glycan strand length, degree of crosslinking, type of crosslinking and other peptidoglycan modifications can be quantified using this approach.
33654919	3	17	theme	Certain	402:408	arg1	composition					458:468	muropeptide composition	446:468	muropeptide composition	446:468	Certain features of the cell wall including muropeptide composition, glycan strand length, degree of crosslinking, type of crosslinking and other peptidoglycan modifications can be quantified using this approach.
33654919	3	17	theme	Certain	402:408	arg1	degree					493:498	degree	493:498	degree of crosslinking	493:514	Certain features of the cell wall including muropeptide composition, glycan strand length, degree of crosslinking, type of crosslinking and other peptidoglycan modifications can be quantified using this approach.
33654919	3	17	theme	Certain	402:408	arg1	features					410:417	Certain features	402:417	Certain features of the cell wall including muropeptide composition, glycan strand length, degree of crosslinking, type of crosslinking and other peptidoglycan modifications	402:574	Certain features of the cell wall including muropeptide composition, glycan strand length, degree of crosslinking, type of crosslinking and other peptidoglycan modifications can be quantified using this approach.
33654919	3	17	theme	Certain	402:408	arg1	modifications					562:574	other peptidoglycan modifications	542:574	other peptidoglycan modifications	542:574	Certain features of the cell wall including muropeptide composition, glycan strand length, degree of crosslinking, type of crosslinking and other peptidoglycan modifications can be quantified using this approach.
33654919	3	17	theme	Certain	402:408	arg1	length					485:490	glycan strand length	471:490	glycan strand length	471:490	Certain features of the cell wall including muropeptide composition, glycan strand length, degree of crosslinking, type of crosslinking and other peptidoglycan modifications can be quantified using this approach.
33654919	2	18	theme	muropeptide	292:302	arg1	subunits					304:311	its muropeptide subunits	288:311	its muropeptide subunits	288:311	Cell wall composition can be best characterized at the molecular level by digesting the peptidoglycan murein polymer into its muropeptide subunits and quantifying the abundance of muropeptides using high-pressure liquid chromatography.
33654919	4	19	theme	environmental	812:824	arg1	perturbations					837:849	environmental or genetic perturbations	812:849	environmental or genetic perturbations	812:849	Well-established protocols provide us with highly-resolved and quantitatively reproducible chromatographic data, which can be used to investigate bacterial cell wall composition under a variety of environmental or genetic perturbations.
33654919	2	20	theme	liquid	379:384	arg1	chromatography					386:399	high-pressure liquid chromatography	365:399	high-pressure liquid chromatography	365:399	Cell wall composition can be best characterized at the molecular level by digesting the peptidoglycan murein polymer into its muropeptide subunits and quantifying the abundance of muropeptides using high-pressure liquid chromatography.
33654919	5	21	theme	muropeptide	906:916	arg1	samples					918:924	muropeptide samples	906:924	muropeptide samples	906:924	The method described here enables the purification of muropeptide samples, their quantification by HPLC, and fraction collection for peak identification by mass spectrometry.
33654919	1	22	theme	sacculus	104:111	arg1	peptidoglycan					90:102	The peptidoglycan sacculus	86:111	The peptidoglycan sacculus	86:111	The peptidoglycan sacculus, or cell wall, is what defines bacterial cell shape.
33654919	3	23	theme	strand	478:483	arg1	length					485:490	glycan strand length	471:490	glycan strand length	471:490	Certain features of the cell wall including muropeptide composition, glycan strand length, degree of crosslinking, type of crosslinking and other peptidoglycan modifications can be quantified using this approach.
33654919	0	24	theme	HPLC	17:20	arg1	Analysis					22:29	HPLC Analysis	17:29	HPLC Analysis	17:29	Purification and HPLC Analysis of Cell Wall Muropeptides from Caulobacter crescentus.
33654919	4	25	theme	genetic	829:835	arg1	perturbations					837:849	environmental or genetic perturbations	812:849	environmental or genetic perturbations	812:849	Well-established protocols provide us with highly-resolved and quantitatively reproducible chromatographic data, which can be used to investigate bacterial cell wall composition under a variety of environmental or genetic perturbations.
33654919	2	26	theme	Cell	166:169	arg1	composition					176:186	Cell wall composition	166:186	Cell wall composition	166:186	Cell wall composition can be best characterized at the molecular level by digesting the peptidoglycan murein polymer into its muropeptide subunits and quantifying the abundance of muropeptides using high-pressure liquid chromatography.
33654919	5	27	theme	fraction	961:968	arg1	collection					970:979	fraction collection	961:979	fraction collection for peak identification by mass spectrometry	961:1024	The method described here enables the purification of muropeptide samples, their quantification by HPLC, and fraction collection for peak identification by mass spectrometry.
33654919	4	28	theme	highly-resolved	658:672	arg1	data					722:725	highly-resolved and quantitatively reproducible chromatographic data	658:725	highly-resolved and quantitatively reproducible chromatographic data	658:725	Well-established protocols provide us with highly-resolved and quantitatively reproducible chromatographic data, which can be used to investigate bacterial cell wall composition under a variety of environmental or genetic perturbations.
33654919	0	29	theme	Cell	34:37	arg1	Muropeptides					44:55	Cell Wall Muropeptides	34:55	Cell Wall Muropeptides	34:55	Purification and HPLC Analysis of Cell Wall Muropeptides from Caulobacter crescentus.
33654919	3	30	theme	crosslinking	503:514	arg1	modifications					562:574	other peptidoglycan modifications	542:574	other peptidoglycan modifications	542:574	Certain features of the cell wall including muropeptide composition, glycan strand length, degree of crosslinking, type of crosslinking and other peptidoglycan modifications can be quantified using this approach.
33654919	3	30	theme	crosslinking	503:514	arg1	type					517:520	type	517:520	type of crosslinking	517:536	Certain features of the cell wall including muropeptide composition, glycan strand length, degree of crosslinking, type of crosslinking and other peptidoglycan modifications can be quantified using this approach.
33654919	3	30	theme	crosslinking	503:514	arg1	composition					458:468	muropeptide composition	446:468	muropeptide composition	446:468	Certain features of the cell wall including muropeptide composition, glycan strand length, degree of crosslinking, type of crosslinking and other peptidoglycan modifications can be quantified using this approach.
33654919	3	30	theme	crosslinking	503:514	arg1	degree					493:498	degree	493:498	degree of crosslinking	493:514	Certain features of the cell wall including muropeptide composition, glycan strand length, degree of crosslinking, type of crosslinking and other peptidoglycan modifications can be quantified using this approach.
33654919	3	30	theme	crosslinking	503:514	arg1	length					485:490	glycan strand length	471:490	glycan strand length	471:490	Certain features of the cell wall including muropeptide composition, glycan strand length, degree of crosslinking, type of crosslinking and other peptidoglycan modifications can be quantified using this approach.
33654919	0	31	from	crescentus	74:83	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification and HPLC Analysis of Cell Wall Muropeptides from Caulobacter crescentus.
33654919	0	31	from	crescentus	74:83	arg1	Analysis					22:29	HPLC Analysis	17:29	HPLC Analysis	17:29	Purification and HPLC Analysis of Cell Wall Muropeptides from Caulobacter crescentus.
33654919	4	32	theme	reproducible	693:704	arg1	data					722:725	highly-resolved and quantitatively reproducible chromatographic data	658:725	highly-resolved and quantitatively reproducible chromatographic data	658:725	Well-established protocols provide us with highly-resolved and quantitatively reproducible chromatographic data, which can be used to investigate bacterial cell wall composition under a variety of environmental or genetic perturbations.
33654919	3	33	theme	muropeptide	446:456	arg1	composition					458:468	muropeptide composition	446:468	muropeptide composition	446:468	Certain features of the cell wall including muropeptide composition, glycan strand length, degree of crosslinking, type of crosslinking and other peptidoglycan modifications can be quantified using this approach.
33654919	3	34	theme	cell	426:429	arg1	wall					431:434	the cell wall	422:434	the cell wall	422:434	Certain features of the cell wall including muropeptide composition, glycan strand length, degree of crosslinking, type of crosslinking and other peptidoglycan modifications can be quantified using this approach.
33654919	5	35	theme	samples	918:924	arg1	purification					890:901	the purification	886:901	the purification of muropeptide samples	886:924	The method described here enables the purification of muropeptide samples, their quantification by HPLC, and fraction collection for peak identification by mass spectrometry.
33654919	5	35	theme	samples	918:924	arg1	quantification					933:946	their quantification	927:946	their quantification by HPLC	927:954	The method described here enables the purification of muropeptide samples, their quantification by HPLC, and fraction collection for peak identification by mass spectrometry.
33654919	5	35	theme	samples	918:924	arg1	collection					970:979	fraction collection	961:979	fraction collection for peak identification by mass spectrometry	961:1024	The method described here enables the purification of muropeptide samples, their quantification by HPLC, and fraction collection for peak identification by mass spectrometry.
33654919	3	36	theme	glycan	471:476	arg1	length					485:490	glycan strand length	471:490	glycan strand length	471:490	Certain features of the cell wall including muropeptide composition, glycan strand length, degree of crosslinking, type of crosslinking and other peptidoglycan modifications can be quantified using this approach.
33654919	0	37	theme	Muropeptides	44:55	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification and HPLC Analysis of Cell Wall Muropeptides from Caulobacter crescentus.
33654919	0	37	theme	Muropeptides	44:55	arg1	Analysis					22:29	HPLC Analysis	17:29	HPLC Analysis	17:29	Purification and HPLC Analysis of Cell Wall Muropeptides from Caulobacter crescentus.
33654919	4	38	theme	bacterial	761:769	arg1	composition					781:791	bacterial cell wall composition	761:791	bacterial cell wall composition	761:791	Well-established protocols provide us with highly-resolved and quantitatively reproducible chromatographic data, which can be used to investigate bacterial cell wall composition under a variety of environmental or genetic perturbations.
33654919	0	39	theme	Wall	39:42	arg1	Muropeptides					44:55	Cell Wall Muropeptides	34:55	Cell Wall Muropeptides	34:55	Purification and HPLC Analysis of Cell Wall Muropeptides from Caulobacter crescentus.
33654919	1	40	theme	cell	117:120	arg1	wall					122:125	cell wall	117:125	cell wall	117:125	The peptidoglycan sacculus, or cell wall, is what defines bacterial cell shape.
33654919	4	41	theme	wall	776:779	arg1	composition					781:791	bacterial cell wall composition	761:791	bacterial cell wall composition	761:791	Well-established protocols provide us with highly-resolved and quantitatively reproducible chromatographic data, which can be used to investigate bacterial cell wall composition under a variety of environmental or genetic perturbations.
33654919	4	42	theme	perturbations	837:849	arg1	perturbations					837:849	environmental or genetic perturbations	812:849	environmental or genetic perturbations	812:849	Well-established protocols provide us with highly-resolved and quantitatively reproducible chromatographic data, which can be used to investigate bacterial cell wall composition under a variety of environmental or genetic perturbations.
33654919	4	42	theme	perturbations	837:849	arg1	variety					801:807	a variety	799:807	a variety of environmental or genetic perturbations	799:849	Well-established protocols provide us with highly-resolved and quantitatively reproducible chromatographic data, which can be used to investigate bacterial cell wall composition under a variety of environmental or genetic perturbations.
33654919	4	43	theme	cell	771:774	arg1	composition					781:791	bacterial cell wall composition	761:791	bacterial cell wall composition	761:791	Well-established protocols provide us with highly-resolved and quantitatively reproducible chromatographic data, which can be used to investigate bacterial cell wall composition under a variety of environmental or genetic perturbations.
33654919	5	44	theme	peak	985:988	arg1	identification					990:1003	peak identification	985:1003	peak identification by mass spectrometry	985:1024	The method described here enables the purification of muropeptide samples, their quantification by HPLC, and fraction collection for peak identification by mass spectrometry.
33654919	0	45	theme	Caulobacter	62:72	arg1	crescentus					74:83	Caulobacter crescentus	62:83	Caulobacter crescentus	62:83	Purification and HPLC Analysis of Cell Wall Muropeptides from Caulobacter crescentus.
33654919	2	46	theme	wall	171:174	arg1	composition					176:186	Cell wall composition	166:186	Cell wall composition	166:186	Cell wall composition can be best characterized at the molecular level by digesting the peptidoglycan murein polymer into its muropeptide subunits and quantifying the abundance of muropeptides using high-pressure liquid chromatography.
33654919	3	47	mod	modifications	562:574	arg3	peptidoglycan					548:560	other peptidoglycan modifications	542:574	other peptidoglycan modifications	542:574	Certain features of the cell wall including muropeptide composition, glycan strand length, degree of crosslinking, type of crosslinking and other peptidoglycan modifications can be quantified using this approach.
33654919	3	47	mod	modifications	562:574	arg1	crosslinking					503:514	crosslinking	503:514	crosslinking	503:514	Certain features of the cell wall including muropeptide composition, glycan strand length, degree of crosslinking, type of crosslinking and other peptidoglycan modifications can be quantified using this approach.
33654919	3	47	mod	modifications	562:574	arg1	crosslinking					525:536	crosslinking	525:536	crosslinking	525:536	Certain features of the cell wall including muropeptide composition, glycan strand length, degree of crosslinking, type of crosslinking and other peptidoglycan modifications can be quantified using this approach.
33654919	2	48	theme	high-pressure	365:377	arg1	chromatography					386:399	high-pressure liquid chromatography	365:399	high-pressure liquid chromatography	365:399	Cell wall composition can be best characterized at the molecular level by digesting the peptidoglycan murein polymer into its muropeptide subunits and quantifying the abundance of muropeptides using high-pressure liquid chromatography.
33654919	6	49	theme	multiple	1251:1258	arg1	samples					1260:1266	multiple samples	1251:1266	multiple samples	1251:1266	Although the methods for peptidoglycan purification and HPLC analysis have been previously published, our method includes important details on how to re-equilibrate the column between runs to allow for automated analysis of multiple samples.
33654919	2	50	theme	molecular	221:229	arg1	level					231:235	the molecular level	217:235	the molecular level	217:235	Cell wall composition can be best characterized at the molecular level by digesting the peptidoglycan murein polymer into its muropeptide subunits and quantifying the abundance of muropeptides using high-pressure liquid chromatography.
33654919	3	51	theme	crosslinking	525:536	arg1	modifications					562:574	other peptidoglycan modifications	542:574	other peptidoglycan modifications	542:574	Certain features of the cell wall including muropeptide composition, glycan strand length, degree of crosslinking, type of crosslinking and other peptidoglycan modifications can be quantified using this approach.
33654919	3	51	theme	crosslinking	525:536	arg1	type					517:520	type	517:520	type of crosslinking	517:536	Certain features of the cell wall including muropeptide composition, glycan strand length, degree of crosslinking, type of crosslinking and other peptidoglycan modifications can be quantified using this approach.
33654919	3	51	theme	crosslinking	525:536	arg1	composition					458:468	muropeptide composition	446:468	muropeptide composition	446:468	Certain features of the cell wall including muropeptide composition, glycan strand length, degree of crosslinking, type of crosslinking and other peptidoglycan modifications can be quantified using this approach.
33654919	3	51	theme	crosslinking	525:536	arg1	degree					493:498	degree	493:498	degree of crosslinking	493:514	Certain features of the cell wall including muropeptide composition, glycan strand length, degree of crosslinking, type of crosslinking and other peptidoglycan modifications can be quantified using this approach.
33654919	3	51	theme	crosslinking	525:536	arg1	length					485:490	glycan strand length	471:490	glycan strand length	471:490	Certain features of the cell wall including muropeptide composition, glycan strand length, degree of crosslinking, type of crosslinking and other peptidoglycan modifications can be quantified using this approach.
31106972	4	0	theme	DNA	871:873	arg1	sequencing					875:884	microbial 16S ribosomal DNA sequencing	847:884	microbial 16S ribosomal DNA sequencing	847:884	METHODS Plasma samples from 2 cohorts, one with 18 unrelated healthy controls and 18 first-degree relatives and the other with 19 healthy controls and 21 SLE patients, were assessed for autoantibody levels by autoantigen microarray analysis, measurement of lipopolysaccharide (LPS) levels by Limulus amebocyte assay, and determination of microbiome composition by microbial 16S ribosomal DNA sequencing.
31106972	4	1	theme	Plasma	491:496	arg1	samples					498:504	METHODS Plasma samples	483:504	METHODS Plasma samples from 2 cohorts, one with 18 unrelated healthy controls and 18 first-degree relatives and the other with 19 healthy controls and 21 SLE patients,	483:649	METHODS Plasma samples from 2 cohorts, one with 18 unrelated healthy controls and 18 first-degree relatives and the other with 19 healthy controls and 21 SLE patients, were assessed for autoantibody levels by autoantigen microarray analysis, measurement of lipopolysaccharide (LPS) levels by Limulus amebocyte assay, and determination of microbiome composition by microbial 16S ribosomal DNA sequencing.
31106972	6	2	theme	=	1116:1116	arg1	P					1114:1114	P = 0.02	1114:1121	P = 0.02	1114:1121	Parents and children of lupus patients exhibited elevated plasma LPS levels compared to controls (P = 0.02).
31106972	6	2	theme	=	1116:1116	arg1	controls					1104:1111	controls	1104:1111	controls (P = 0.02)	1104:1122	Parents and children of lupus patients exhibited elevated plasma LPS levels compared to controls (P = 0.02).
31106972	4	3	from	cohorts	513:519	arg1	samples					498:504	METHODS Plasma samples	483:504	METHODS Plasma samples from 2 cohorts, one with 18 unrelated healthy controls and 18 first-degree relatives and the other with 19 healthy controls and 21 SLE patients,	483:649	METHODS Plasma samples from 2 cohorts, one with 18 unrelated healthy controls and 18 first-degree relatives and the other with 19 healthy controls and 21 SLE patients, were assessed for autoantibody levels by autoantigen microarray analysis, measurement of lipopolysaccharide (LPS) levels by Limulus amebocyte assay, and determination of microbiome composition by microbial 16S ribosomal DNA sequencing.
31106972	10	4	from	bacteria	1883:1890	arg1	cohorts					1900:1906	both cohorts	1895:1906	both cohorts	1895:1906	Bacteria in the Paenibacillus genus were the only overlapping differentially abundant bacteria in both cohorts, and were reduced in first-degree relatives (adjusted P [Padj ] = 2.13 × 10-12 ) and SLE patients (Padj = 0.008) but elevated in controls.
31106972	9	5	theme	first-degree	1635:1646	arg1	relatives					1648:1656	first-degree relatives	1635:1656	first-degree relatives	1635:1656	The majority of bacteria that were differentially abundant between unrelated healthy controls and first-degree relatives were in the Firmicutes phylum, while differences in bacteria from several phyla were identified between healthy controls and SLE patients.
31106972	7	6	theme	Plasma	1125:1130	arg1	levels					1136:1141	Plasma LPS levels	1125:1141	Plasma LPS levels	1125:1141	Plasma LPS levels positively correlated with plasma anti-double-stranded DNA IgG levels in first-degree relatives (r = 0.51, P = 0.03), but not in SLE patients.
31106972	11	7	dep	CONCLUSIONS	2047:2057	arg1	indicate					2073:2080	indicate	2073:2080	indicate a possible role of plasma microbial translocation and microbiome composition in influencing autoantibody development in SLE	2073:2204	CONCLUSIONS These results indicate a possible role of plasma microbial translocation and microbiome composition in influencing autoantibody development in SLE.
31106972	4	8	theme	16S	857:859	arg1	sequencing					875:884	microbial 16S ribosomal DNA sequencing	847:884	microbial 16S ribosomal DNA sequencing	847:884	METHODS Plasma samples from 2 cohorts, one with 18 unrelated healthy controls and 18 first-degree relatives and the other with 19 healthy controls and 21 SLE patients, were assessed for autoantibody levels by autoantigen microarray analysis, measurement of lipopolysaccharide (LPS) levels by Limulus amebocyte assay, and determination of microbiome composition by microbial 16S ribosomal DNA sequencing.
31106972	5	9	theme	autoantibody	962:973	arg1	levels					975:980	increased plasma autoantibody levels	945:980	increased plasma autoantibody levels	945:980	RESULTS First-degree relatives and SLE patients exhibited increased plasma autoantibody levels compared to their control groups.
31106972	10	10	theme	adjusted	1953:1960	arg1	relatives					1942:1950	first-degree relatives	1929:1950	first-degree relatives (adjusted P [Padj ] = 2.13 × 10-12 )	1929:1987	Bacteria in the Paenibacillus genus were the only overlapping differentially abundant bacteria in both cohorts, and were reduced in first-degree relatives (adjusted P [Padj ] = 2.13 × 10-12 ) and SLE patients (Padj = 0.008) but elevated in controls.
31106972	10	10	theme	adjusted	1953:1960	arg1	×					1979:1979	adjusted P [Padj ] = 2.13 × 10-12	1953:1985	adjusted P [Padj ] = 2.13 × 10-12	1953:1985	Bacteria in the Paenibacillus genus were the only overlapping differentially abundant bacteria in both cohorts, and were reduced in first-degree relatives (adjusted P [Padj ] = 2.13 × 10-12 ) and SLE patients (Padj = 0.008) but elevated in controls.
31106972	0	11	theme	Systemic	117:124	arg1	Erythematosus					132:144	Systemic Lupus Erythematosus	117:144	Systemic Lupus Erythematosus Patients	117:153	A Link Between Plasma Microbial Translocation, Microbiome, and Autoantibody Development in First-Degree Relatives of Systemic Lupus Erythematosus Patients.
31106972	2	12	theme	autoantibody	304:315	arg1	formation					317:325	autoantibody formation	304:325	autoantibody formation in SLE	304:332	However, the events underlying autoantibody formation in SLE remain unclear.
31106972	8	13	theme	=	1450:1450	arg1	0.004					1452:1456	P = 0.004	1448:1456	P = 0.004	1448:1456	Circulating microbiome analysis revealed that first-degree relatives had significantly reduced microbiome diversity compared to their controls (observed species, P = 0.004; Chao1 index, P = 0.005), but this reduction was not observed in SLE patients.
31106972	4	14	theme	SLE	637:639	arg1	patients					641:648	21 SLE patients	634:648	21 SLE patients	634:648	METHODS Plasma samples from 2 cohorts, one with 18 unrelated healthy controls and 18 first-degree relatives and the other with 19 healthy controls and 21 SLE patients, were assessed for autoantibody levels by autoantigen microarray analysis, measurement of lipopolysaccharide (LPS) levels by Limulus amebocyte assay, and determination of microbiome composition by microbial 16S ribosomal DNA sequencing.
31106972	0	15	theme	Erythematosus	132:144	arg1	Patients					146:153	Systemic Lupus Erythematosus Patients	117:153	Systemic Lupus Erythematosus Patients	117:153	A Link Between Plasma Microbial Translocation, Microbiome, and Autoantibody Development in First-Degree Relatives of Systemic Lupus Erythematosus Patients.
31106972	7	16	theme	anti-double-stranded	1177:1196	arg1	levels					1206:1211	plasma anti-double-stranded DNA IgG levels	1170:1211	plasma anti-double-stranded DNA IgG levels in first-degree relatives (r = 0.51, P = 0.03), but not in SLE patients	1170:1283	Plasma LPS levels positively correlated with plasma anti-double-stranded DNA IgG levels in first-degree relatives (r = 0.51, P = 0.03), but not in SLE patients.
31106972	4	17	theme	microarray	704:713	arg1	analysis					715:722	autoantigen microarray analysis	692:722	autoantigen microarray analysis	692:722	METHODS Plasma samples from 2 cohorts, one with 18 unrelated healthy controls and 18 first-degree relatives and the other with 19 healthy controls and 21 SLE patients, were assessed for autoantibody levels by autoantigen microarray analysis, measurement of lipopolysaccharide (LPS) levels by Limulus amebocyte assay, and determination of microbiome composition by microbial 16S ribosomal DNA sequencing.
31106972	10	18	theme	=	1972:1972	arg1	relatives					1942:1950	first-degree relatives	1929:1950	first-degree relatives (adjusted P [Padj ] = 2.13 × 10-12 )	1929:1987	Bacteria in the Paenibacillus genus were the only overlapping differentially abundant bacteria in both cohorts, and were reduced in first-degree relatives (adjusted P [Padj ] = 2.13 × 10-12 ) and SLE patients (Padj = 0.008) but elevated in controls.
31106972	10	18	theme	=	1972:1972	arg1	×					1979:1979	adjusted P [Padj ] = 2.13 × 10-12	1953:1985	adjusted P [Padj ] = 2.13 × 10-12	1953:1985	Bacteria in the Paenibacillus genus were the only overlapping differentially abundant bacteria in both cohorts, and were reduced in first-degree relatives (adjusted P [Padj ] = 2.13 × 10-12 ) and SLE patients (Padj = 0.008) but elevated in controls.
31106972	4	19	with	other	599:603	arg1	controls					621:628	19 healthy controls	610:628	19 healthy controls	610:628	METHODS Plasma samples from 2 cohorts, one with 18 unrelated healthy controls and 18 first-degree relatives and the other with 19 healthy controls and 21 SLE patients, were assessed for autoantibody levels by autoantigen microarray analysis, measurement of lipopolysaccharide (LPS) levels by Limulus amebocyte assay, and determination of microbiome composition by microbial 16S ribosomal DNA sequencing.
31106972	4	19	with	other	599:603	arg1	patients					641:648	21 SLE patients	634:648	21 SLE patients	634:648	METHODS Plasma samples from 2 cohorts, one with 18 unrelated healthy controls and 18 first-degree relatives and the other with 19 healthy controls and 21 SLE patients, were assessed for autoantibody levels by autoantigen microarray analysis, measurement of lipopolysaccharide (LPS) levels by Limulus amebocyte assay, and determination of microbiome composition by microbial 16S ribosomal DNA sequencing.
31106972	8	20	theme	Circulating	1286:1296	arg1	analysis					1309:1316	Circulating microbiome analysis	1286:1316	Circulating microbiome analysis	1286:1316	Circulating microbiome analysis revealed that first-degree relatives had significantly reduced microbiome diversity compared to their controls (observed species, P = 0.004; Chao1 index, P = 0.005), but this reduction was not observed in SLE patients.
31106972	10	21	theme	SLE	1993:1995	arg1	0.008					2014:2018	Padj = 0.008	2007:2018	Padj = 0.008	2007:2018	Bacteria in the Paenibacillus genus were the only overlapping differentially abundant bacteria in both cohorts, and were reduced in first-degree relatives (adjusted P [Padj ] = 2.13 × 10-12 ) and SLE patients (Padj = 0.008) but elevated in controls.
31106972	10	21	theme	SLE	1993:1995	arg1	patients					1997:2004	SLE patients	1993:2004	SLE patients (Padj = 0.008)	1993:2019	Bacteria in the Paenibacillus genus were the only overlapping differentially abundant bacteria in both cohorts, and were reduced in first-degree relatives (adjusted P [Padj ] = 2.13 × 10-12 ) and SLE patients (Padj = 0.008) but elevated in controls.
31106972	5	22	theme	increased	945:953	arg1	levels					975:980	increased plasma autoantibody levels	945:980	increased plasma autoantibody levels	945:980	RESULTS First-degree relatives and SLE patients exhibited increased plasma autoantibody levels compared to their control groups.
31106972	6	23	theme	patients	1046:1053	arg1	children					1028:1035	children	1028:1035	children	1028:1035	Parents and children of lupus patients exhibited elevated plasma LPS levels compared to controls (P = 0.02).
31106972	6	23	theme	patients	1046:1053	arg1	Parents					1016:1022	Parents	1016:1022	Parents	1016:1022	Parents and children of lupus patients exhibited elevated plasma LPS levels compared to controls (P = 0.02).
31106972	4	24	theme	autoantibody	669:680	arg1	levels					682:687	autoantibody levels	669:687	autoantibody levels	669:687	METHODS Plasma samples from 2 cohorts, one with 18 unrelated healthy controls and 18 first-degree relatives and the other with 19 healthy controls and 21 SLE patients, were assessed for autoantibody levels by autoantigen microarray analysis, measurement of lipopolysaccharide (LPS) levels by Limulus amebocyte assay, and determination of microbiome composition by microbial 16S ribosomal DNA sequencing.
31106972	5	25	theme	First-degree	895:906	arg1	relatives					908:916	First-degree relatives	895:916	First-degree relatives	895:916	RESULTS First-degree relatives and SLE patients exhibited increased plasma autoantibody levels compared to their control groups.
31106972	2	26	from	formation	317:325	arg1	SLE					330:332	SLE	330:332	SLE	330:332	However, the events underlying autoantibody formation in SLE remain unclear.
31106972	10	27	theme	=	2012:2012	arg1	0.008					2014:2018	Padj = 0.008	2007:2018	Padj = 0.008	2007:2018	Bacteria in the Paenibacillus genus were the only overlapping differentially abundant bacteria in both cohorts, and were reduced in first-degree relatives (adjusted P [Padj ] = 2.13 × 10-12 ) and SLE patients (Padj = 0.008) but elevated in controls.
31106972	10	27	theme	=	2012:2012	arg1	patients					1997:2004	SLE patients	1993:2004	SLE patients (Padj = 0.008)	1993:2019	Bacteria in the Paenibacillus genus were the only overlapping differentially abundant bacteria in both cohorts, and were reduced in first-degree relatives (adjusted P [Padj ] = 2.13 × 10-12 ) and SLE patients (Padj = 0.008) but elevated in controls.
31106972	0	28	theme	Autoantibody	63:74	arg1	Development					76:86	Autoantibody Development	63:86	Autoantibody Development	63:86	A Link Between Plasma Microbial Translocation, Microbiome, and Autoantibody Development in First-Degree Relatives of Systemic Lupus Erythematosus Patients.
31106972	1	29	theme	lupus	175:179	arg1	SLE					196:198	SLE	196:198	SLE	196:198	OBJECTIVE Systemic lupus erythematosus (SLE) is characterized by the production of antibodies against self antigens.
31106972	1	29	theme	lupus	175:179	arg1	erythematosus					181:193	OBJECTIVE Systemic lupus erythematosus	156:193	OBJECTIVE Systemic lupus erythematosus (SLE)	156:199	OBJECTIVE Systemic lupus erythematosus (SLE) is characterized by the production of antibodies against self antigens.
31106972	9	30	from	differences	1695:1705	arg1	bacteria					1710:1717	bacteria	1710:1717	bacteria from several phyla	1710:1736	The majority of bacteria that were differentially abundant between unrelated healthy controls and first-degree relatives were in the Firmicutes phylum, while differences in bacteria from several phyla were identified between healthy controls and SLE patients.
31106972	9	30	from	differences	1695:1705	arg1	phyla					1732:1736	several phyla	1724:1736	several phyla	1724:1736	The majority of bacteria that were differentially abundant between unrelated healthy controls and first-degree relatives were in the Firmicutes phylum, while differences in bacteria from several phyla were identified between healthy controls and SLE patients.
31106972	8	31	contain	had	1355:1357	arg2	diversity					1392:1400	significantly reduced microbiome diversity	1359:1400	significantly reduced microbiome diversity	1359:1400	Circulating microbiome analysis revealed that first-degree relatives had significantly reduced microbiome diversity compared to their controls (observed species, P = 0.004; Chao1 index, P = 0.005), but this reduction was not observed in SLE patients.
31106972	8	31	contain	had	1355:1357	arg1	relatives					1345:1353	first-degree relatives	1332:1353	first-degree relatives	1332:1353	Circulating microbiome analysis revealed that first-degree relatives had significantly reduced microbiome diversity compared to their controls (observed species, P = 0.004; Chao1 index, P = 0.005), but this reduction was not observed in SLE patients.
31106972	7	32	theme	first-degree	1216:1227	arg1	relatives					1229:1237	first-degree relatives	1216:1237	first-degree relatives (r = 0.51, P = 0.03)	1216:1258	Plasma LPS levels positively correlated with plasma anti-double-stranded DNA IgG levels in first-degree relatives (r = 0.51, P = 0.03), but not in SLE patients.
31106972	3	33	from	role	395:398	arg1	SLE					478:480	SLE	478:480	SLE	478:480	This study was undertaken to investigate the role of plasma autoantibody levels, microbial translocation, and the microbiome in SLE.
31106972	7	34	from	levels	1206:1211	arg1	relatives					1229:1237	first-degree relatives	1216:1237	first-degree relatives (r = 0.51, P = 0.03)	1216:1258	Plasma LPS levels positively correlated with plasma anti-double-stranded DNA IgG levels in first-degree relatives (r = 0.51, P = 0.03), but not in SLE patients.
31106972	7	34	from	levels	1206:1211	arg1	patients					1276:1283	SLE patients	1272:1283	SLE patients	1272:1283	Plasma LPS levels positively correlated with plasma anti-double-stranded DNA IgG levels in first-degree relatives (r = 0.51, P = 0.03), but not in SLE patients.
31106972	8	35	theme	microbiome	1381:1390	arg1	diversity					1392:1400	significantly reduced microbiome diversity	1359:1400	significantly reduced microbiome diversity	1359:1400	Circulating microbiome analysis revealed that first-degree relatives had significantly reduced microbiome diversity compared to their controls (observed species, P = 0.004; Chao1 index, P = 0.005), but this reduction was not observed in SLE patients.
31106972	3	36	theme	microbial	431:439	arg1	translocation					441:453	microbial translocation	431:453	microbial translocation	431:453	This study was undertaken to investigate the role of plasma autoantibody levels, microbial translocation, and the microbiome in SLE.
31106972	7	37	dep	=	1252:1252	arg1	r					1240:1240	r	1240:1240	r = 0.51	1240:1247	Plasma LPS levels positively correlated with plasma anti-double-stranded DNA IgG levels in first-degree relatives (r = 0.51, P = 0.03), but not in SLE patients.
31106972	10	38	theme	overlapping	1847:1857	arg1	Bacteria					1797:1804	Bacteria	1797:1804	Bacteria in the Paenibacillus genus	1797:1831	Bacteria in the Paenibacillus genus were the only overlapping differentially abundant bacteria in both cohorts, and were reduced in first-degree relatives (adjusted P [Padj ] = 2.13 × 10-12 ) and SLE patients (Padj = 0.008) but elevated in controls.
31106972	10	38	theme	overlapping	1847:1857	arg1	bacteria					1883:1890	the only overlapping differentially abundant bacteria	1838:1890	the only overlapping differentially abundant bacteria in both cohorts	1838:1906	Bacteria in the Paenibacillus genus were the only overlapping differentially abundant bacteria in both cohorts, and were reduced in first-degree relatives (adjusted P [Padj ] = 2.13 × 10-12 ) and SLE patients (Padj = 0.008) but elevated in controls.
31106972	11	39	theme	microbial	2108:2116	arg1	translocation					2118:2130	plasma microbial translocation	2101:2130	plasma microbial translocation	2101:2130	CONCLUSIONS These results indicate a possible role of plasma microbial translocation and microbiome composition in influencing autoantibody development in SLE.
31106972	4	40	theme	healthy	544:550	arg1	controls					552:559	18 unrelated healthy controls	531:559	18 unrelated healthy controls	531:559	METHODS Plasma samples from 2 cohorts, one with 18 unrelated healthy controls and 18 first-degree relatives and the other with 19 healthy controls and 21 SLE patients, were assessed for autoantibody levels by autoantigen microarray analysis, measurement of lipopolysaccharide (LPS) levels by Limulus amebocyte assay, and determination of microbiome composition by microbial 16S ribosomal DNA sequencing.
31106972	5	41	dep	RESULTS	887:893	arg1	relatives					908:916	First-degree relatives	895:916	First-degree relatives	895:916	RESULTS First-degree relatives and SLE patients exhibited increased plasma autoantibody levels compared to their control groups.
31106972	7	42	theme	DNA	1198:1200	arg1	levels					1206:1211	plasma anti-double-stranded DNA IgG levels	1170:1211	plasma anti-double-stranded DNA IgG levels in first-degree relatives (r = 0.51, P = 0.03), but not in SLE patients	1170:1283	Plasma LPS levels positively correlated with plasma anti-double-stranded DNA IgG levels in first-degree relatives (r = 0.51, P = 0.03), but not in SLE patients.
31106972	6	43	theme	LPS	1081:1083	arg1	levels					1085:1090	elevated plasma LPS levels	1065:1090	elevated plasma LPS levels	1065:1090	Parents and children of lupus patients exhibited elevated plasma LPS levels compared to controls (P = 0.02).
31106972	3	44	theme	levels	423:428	arg1	role					395:398	the role	391:398	the role of plasma autoantibody levels, microbial translocation, and the microbiome in SLE	391:480	This study was undertaken to investigate the role of plasma autoantibody levels, microbial translocation, and the microbiome in SLE.
31106972	8	45	theme	SLE	1523:1525	arg1	patients					1527:1534	SLE patients	1523:1534	SLE patients	1523:1534	Circulating microbiome analysis revealed that first-degree relatives had significantly reduced microbiome diversity compared to their controls (observed species, P = 0.004; Chao1 index, P = 0.005), but this reduction was not observed in SLE patients.
31106972	6	46	theme	plasma	1074:1079	arg1	levels					1085:1090	elevated plasma LPS levels	1065:1090	elevated plasma LPS levels	1065:1090	Parents and children of lupus patients exhibited elevated plasma LPS levels compared to controls (P = 0.02).
31106972	7	47	theme	plasma	1170:1175	arg1	levels					1206:1211	plasma anti-double-stranded DNA IgG levels	1170:1211	plasma anti-double-stranded DNA IgG levels in first-degree relatives (r = 0.51, P = 0.03), but not in SLE patients	1170:1283	Plasma LPS levels positively correlated with plasma anti-double-stranded DNA IgG levels in first-degree relatives (r = 0.51, P = 0.03), but not in SLE patients.
31106972	0	48	theme	Microbial	22:30	arg1	Translocation					32:44	Plasma Microbial Translocation	15:44	Plasma Microbial Translocation	15:44	A Link Between Plasma Microbial Translocation, Microbiome, and Autoantibody Development in First-Degree Relatives of Systemic Lupus Erythematosus Patients.
31106972	8	49	located	observed	1511:1518	arg2	reduction					1493:1501	this reduction	1488:1501	this reduction	1488:1501	Circulating microbiome analysis revealed that first-degree relatives had significantly reduced microbiome diversity compared to their controls (observed species, P = 0.004; Chao1 index, P = 0.005), but this reduction was not observed in SLE patients.
31106972	8	49	located	observed	1511:1518	arg1	patients					1527:1534	SLE patients	1523:1534	SLE patients	1523:1534	Circulating microbiome analysis revealed that first-degree relatives had significantly reduced microbiome diversity compared to their controls (observed species, P = 0.004; Chao1 index, P = 0.005), but this reduction was not observed in SLE patients.
31106972	6	50	theme	elevated	1065:1072	arg1	levels					1085:1090	elevated plasma LPS levels	1065:1090	elevated plasma LPS levels	1065:1090	Parents and children of lupus patients exhibited elevated plasma LPS levels compared to controls (P = 0.02).
31106972	3	51	theme	plasma	403:408	arg1	levels					423:428	plasma autoantibody levels	403:428	plasma autoantibody levels	403:428	This study was undertaken to investigate the role of plasma autoantibody levels, microbial translocation, and the microbiome in SLE.
31106972	4	52	theme	microbiome	821:830	arg1	composition					832:842	microbiome composition	821:842	microbiome composition	821:842	METHODS Plasma samples from 2 cohorts, one with 18 unrelated healthy controls and 18 first-degree relatives and the other with 19 healthy controls and 21 SLE patients, were assessed for autoantibody levels by autoantigen microarray analysis, measurement of lipopolysaccharide (LPS) levels by Limulus amebocyte assay, and determination of microbiome composition by microbial 16S ribosomal DNA sequencing.
31106972	11	53	theme	plasma	2101:2106	arg1	translocation					2118:2130	plasma microbial translocation	2101:2130	plasma microbial translocation	2101:2130	CONCLUSIONS These results indicate a possible role of plasma microbial translocation and microbiome composition in influencing autoantibody development in SLE.
31106972	7	54	dep	relatives	1229:1237	arg1	=					1252:1252	=	1252:1252	=	1252:1252	Plasma LPS levels positively correlated with plasma anti-double-stranded DNA IgG levels in first-degree relatives (r = 0.51, P = 0.03), but not in SLE patients.
31106972	4	55	theme	amebocyte	783:791	arg1	assay					793:797	Limulus amebocyte assay	775:797	Limulus amebocyte assay	775:797	METHODS Plasma samples from 2 cohorts, one with 18 unrelated healthy controls and 18 first-degree relatives and the other with 19 healthy controls and 21 SLE patients, were assessed for autoantibody levels by autoantigen microarray analysis, measurement of lipopolysaccharide (LPS) levels by Limulus amebocyte assay, and determination of microbiome composition by microbial 16S ribosomal DNA sequencing.
31106972	9	56	from	phyla	1732:1736	arg1	bacteria					1710:1717	bacteria	1710:1717	bacteria from several phyla	1710:1736	The majority of bacteria that were differentially abundant between unrelated healthy controls and first-degree relatives were in the Firmicutes phylum, while differences in bacteria from several phyla were identified between healthy controls and SLE patients.
31106972	9	56	from	phyla	1732:1736	arg1	differences					1695:1705	differences	1695:1705	differences in bacteria from several phyla	1695:1736	The majority of bacteria that were differentially abundant between unrelated healthy controls and first-degree relatives were in the Firmicutes phylum, while differences in bacteria from several phyla were identified between healthy controls and SLE patients.
31106972	1	57	theme	OBJECTIVE	156:164	arg1	SLE					196:198	SLE	196:198	SLE	196:198	OBJECTIVE Systemic lupus erythematosus (SLE) is characterized by the production of antibodies against self antigens.
31106972	1	57	theme	OBJECTIVE	156:164	arg1	erythematosus					181:193	OBJECTIVE Systemic lupus erythematosus	156:193	OBJECTIVE Systemic lupus erythematosus (SLE)	156:199	OBJECTIVE Systemic lupus erythematosus (SLE) is characterized by the production of antibodies against self antigens.
31106972	10	58	theme	abundant	1874:1881	arg1	Bacteria					1797:1804	Bacteria	1797:1804	Bacteria in the Paenibacillus genus	1797:1831	Bacteria in the Paenibacillus genus were the only overlapping differentially abundant bacteria in both cohorts, and were reduced in first-degree relatives (adjusted P [Padj ] = 2.13 × 10-12 ) and SLE patients (Padj = 0.008) but elevated in controls.
31106972	10	58	theme	abundant	1874:1881	arg1	bacteria					1883:1890	the only overlapping differentially abundant bacteria	1838:1890	the only overlapping differentially abundant bacteria in both cohorts	1838:1906	Bacteria in the Paenibacillus genus were the only overlapping differentially abundant bacteria in both cohorts, and were reduced in first-degree relatives (adjusted P [Padj ] = 2.13 × 10-12 ) and SLE patients (Padj = 0.008) but elevated in controls.
31106972	8	59	theme	P	1448:1448	arg1	0.004					1452:1456	P = 0.004	1448:1456	P = 0.004	1448:1456	Circulating microbiome analysis revealed that first-degree relatives had significantly reduced microbiome diversity compared to their controls (observed species, P = 0.004; Chao1 index, P = 0.005), but this reduction was not observed in SLE patients.
31106972	1	60	theme	antibodies	239:248	arg1	production					225:234	the production	221:234	the production of antibodies against self antigens	221:270	OBJECTIVE Systemic lupus erythematosus (SLE) is characterized by the production of antibodies against self antigens.
31106972	11	61	theme	composition	2147:2157	arg1	role					2093:2096	a possible role	2082:2096	a possible role of plasma microbial translocation and microbiome composition in influencing autoantibody development in SLE	2082:2204	CONCLUSIONS These results indicate a possible role of plasma microbial translocation and microbiome composition in influencing autoantibody development in SLE.
31106972	9	62	theme	healthy	1614:1620	arg1	controls					1622:1629	unrelated healthy controls	1604:1629	unrelated healthy controls	1604:1629	The majority of bacteria that were differentially abundant between unrelated healthy controls and first-degree relatives were in the Firmicutes phylum, while differences in bacteria from several phyla were identified between healthy controls and SLE patients.
31106972	7	63	theme	LPS	1132:1134	arg1	levels					1136:1141	Plasma LPS levels	1125:1141	Plasma LPS levels	1125:1141	Plasma LPS levels positively correlated with plasma anti-double-stranded DNA IgG levels in first-degree relatives (r = 0.51, P = 0.03), but not in SLE patients.
31106972	1	64	theme	self	258:261	arg1	antigens					263:270	self antigens	258:270	self antigens	258:270	OBJECTIVE Systemic lupus erythematosus (SLE) is characterized by the production of antibodies against self antigens.
31106972	4	65	theme	ribosomal	861:869	arg1	sequencing					875:884	microbial 16S ribosomal DNA sequencing	847:884	microbial 16S ribosomal DNA sequencing	847:884	METHODS Plasma samples from 2 cohorts, one with 18 unrelated healthy controls and 18 first-degree relatives and the other with 19 healthy controls and 21 SLE patients, were assessed for autoantibody levels by autoantigen microarray analysis, measurement of lipopolysaccharide (LPS) levels by Limulus amebocyte assay, and determination of microbiome composition by microbial 16S ribosomal DNA sequencing.
31106972	4	66	theme	first-degree	568:579	arg1	relatives					581:589	18 first-degree relatives	565:589	18 first-degree relatives	565:589	METHODS Plasma samples from 2 cohorts, one with 18 unrelated healthy controls and 18 first-degree relatives and the other with 19 healthy controls and 21 SLE patients, were assessed for autoantibody levels by autoantigen microarray analysis, measurement of lipopolysaccharide (LPS) levels by Limulus amebocyte assay, and determination of microbiome composition by microbial 16S ribosomal DNA sequencing.
31106972	10	67	theme	first-degree	1929:1940	arg1	×					1979:1979	adjusted P [Padj ] = 2.13 × 10-12	1953:1985	adjusted P [Padj ] = 2.13 × 10-12	1953:1985	Bacteria in the Paenibacillus genus were the only overlapping differentially abundant bacteria in both cohorts, and were reduced in first-degree relatives (adjusted P [Padj ] = 2.13 × 10-12 ) and SLE patients (Padj = 0.008) but elevated in controls.
31106972	10	67	theme	first-degree	1929:1940	arg1	relatives					1942:1950	first-degree relatives	1929:1950	first-degree relatives (adjusted P [Padj ] = 2.13 × 10-12 )	1929:1987	Bacteria in the Paenibacillus genus were the only overlapping differentially abundant bacteria in both cohorts, and were reduced in first-degree relatives (adjusted P [Padj ] = 2.13 × 10-12 ) and SLE patients (Padj = 0.008) but elevated in controls.
31106972	4	68	theme	composition	832:842	arg1	determination					804:816	determination	804:816	determination of microbiome composition by microbial 16S ribosomal DNA sequencing	804:884	METHODS Plasma samples from 2 cohorts, one with 18 unrelated healthy controls and 18 first-degree relatives and the other with 19 healthy controls and 21 SLE patients, were assessed for autoantibody levels by autoantigen microarray analysis, measurement of lipopolysaccharide (LPS) levels by Limulus amebocyte assay, and determination of microbiome composition by microbial 16S ribosomal DNA sequencing.
31106972	4	68	theme	composition	832:842	arg1	measurement					725:735	measurement	725:735	measurement of lipopolysaccharide (LPS) levels by Limulus amebocyte assay	725:797	METHODS Plasma samples from 2 cohorts, one with 18 unrelated healthy controls and 18 first-degree relatives and the other with 19 healthy controls and 21 SLE patients, were assessed for autoantibody levels by autoantigen microarray analysis, measurement of lipopolysaccharide (LPS) levels by Limulus amebocyte assay, and determination of microbiome composition by microbial 16S ribosomal DNA sequencing.
31106972	4	68	theme	composition	832:842	arg1	analysis					715:722	autoantigen microarray analysis	692:722	autoantigen microarray analysis	692:722	METHODS Plasma samples from 2 cohorts, one with 18 unrelated healthy controls and 18 first-degree relatives and the other with 19 healthy controls and 21 SLE patients, were assessed for autoantibody levels by autoantigen microarray analysis, measurement of lipopolysaccharide (LPS) levels by Limulus amebocyte assay, and determination of microbiome composition by microbial 16S ribosomal DNA sequencing.
31106972	7	69	theme	=	1242:1242	arg1	r					1240:1240	r	1240:1240	r = 0.51	1240:1247	Plasma LPS levels positively correlated with plasma anti-double-stranded DNA IgG levels in first-degree relatives (r = 0.51, P = 0.03), but not in SLE patients.
31106972	4	70	theme	microbial	847:855	arg1	sequencing					875:884	microbial 16S ribosomal DNA sequencing	847:884	microbial 16S ribosomal DNA sequencing	847:884	METHODS Plasma samples from 2 cohorts, one with 18 unrelated healthy controls and 18 first-degree relatives and the other with 19 healthy controls and 21 SLE patients, were assessed for autoantibody levels by autoantigen microarray analysis, measurement of lipopolysaccharide (LPS) levels by Limulus amebocyte assay, and determination of microbiome composition by microbial 16S ribosomal DNA sequencing.
31106972	0	71	theme	Lupus	126:130	arg1	Erythematosus					132:144	Systemic Lupus Erythematosus	117:144	Systemic Lupus Erythematosus Patients	117:153	A Link Between Plasma Microbial Translocation, Microbiome, and Autoantibody Development in First-Degree Relatives of Systemic Lupus Erythematosus Patients.
31106972	0	72	theme	Patients	146:153	arg1	Relatives					104:112	First-Degree Relatives	91:112	First-Degree Relatives of Systemic Lupus Erythematosus Patients	91:153	A Link Between Plasma Microbial Translocation, Microbiome, and Autoantibody Development in First-Degree Relatives of Systemic Lupus Erythematosus Patients.
31106972	8	73	theme	Chao1	1459:1463	arg1	index					1465:1469	Chao1 index	1459:1469	Chao1 index	1459:1469	Circulating microbiome analysis revealed that first-degree relatives had significantly reduced microbiome diversity compared to their controls (observed species, P = 0.004; Chao1 index, P = 0.005), but this reduction was not observed in SLE patients.
31106972	9	74	theme	healthy	1762:1768	arg1	controls					1770:1777	healthy controls	1762:1777	healthy controls	1762:1777	The majority of bacteria that were differentially abundant between unrelated healthy controls and first-degree relatives were in the Firmicutes phylum, while differences in bacteria from several phyla were identified between healthy controls and SLE patients.
31106972	5	75	theme	SLE	922:924	arg1	patients					926:933	SLE patients	922:933	SLE patients	922:933	RESULTS First-degree relatives and SLE patients exhibited increased plasma autoantibody levels compared to their control groups.
31106972	4	76	theme	autoantigen	692:702	arg1	analysis					715:722	autoantigen microarray analysis	692:722	autoantigen microarray analysis	692:722	METHODS Plasma samples from 2 cohorts, one with 18 unrelated healthy controls and 18 first-degree relatives and the other with 19 healthy controls and 21 SLE patients, were assessed for autoantibody levels by autoantigen microarray analysis, measurement of lipopolysaccharide (LPS) levels by Limulus amebocyte assay, and determination of microbiome composition by microbial 16S ribosomal DNA sequencing.
31106972	8	77	dep	species	1439:1445	arg1	P					1472:1472	P = 0.005	1472:1480	P = 0.005	1472:1480	Circulating microbiome analysis revealed that first-degree relatives had significantly reduced microbiome diversity compared to their controls (observed species, P = 0.004; Chao1 index, P = 0.005), but this reduction was not observed in SLE patients.
31106972	8	77	dep	species	1439:1445	arg1	0.004					1452:1456	P = 0.004	1448:1456	P = 0.004	1448:1456	Circulating microbiome analysis revealed that first-degree relatives had significantly reduced microbiome diversity compared to their controls (observed species, P = 0.004; Chao1 index, P = 0.005), but this reduction was not observed in SLE patients.
31106972	8	77	dep	species	1439:1445	arg1	index					1465:1469	Chao1 index	1459:1469	Chao1 index	1459:1469	Circulating microbiome analysis revealed that first-degree relatives had significantly reduced microbiome diversity compared to their controls (observed species, P = 0.004; Chao1 index, P = 0.005), but this reduction was not observed in SLE patients.
31106972	9	78	theme	several	1724:1730	arg1	phyla					1732:1736	several phyla	1724:1736	several phyla	1724:1736	The majority of bacteria that were differentially abundant between unrelated healthy controls and first-degree relatives were in the Firmicutes phylum, while differences in bacteria from several phyla were identified between healthy controls and SLE patients.
31106972	8	79	theme	=	1474:1474	arg1	P					1472:1472	P = 0.005	1472:1480	P = 0.005	1472:1480	Circulating microbiome analysis revealed that first-degree relatives had significantly reduced microbiome diversity compared to their controls (observed species, P = 0.004; Chao1 index, P = 0.005), but this reduction was not observed in SLE patients.
31106972	10	80	theme	Paenibacillus	1813:1825	arg1	genus					1827:1831	the Paenibacillus genus	1809:1831	the Paenibacillus genus	1809:1831	Bacteria in the Paenibacillus genus were the only overlapping differentially abundant bacteria in both cohorts, and were reduced in first-degree relatives (adjusted P [Padj ] = 2.13 × 10-12 ) and SLE patients (Padj = 0.008) but elevated in controls.
31106972	6	81	theme	lupus	1040:1044	arg1	patients					1046:1053	lupus patients	1040:1053	lupus patients	1040:1053	Parents and children of lupus patients exhibited elevated plasma LPS levels compared to controls (P = 0.02).
31106972	8	82	theme	microbiome	1298:1307	arg1	analysis					1309:1316	Circulating microbiome analysis	1286:1316	Circulating microbiome analysis	1286:1316	Circulating microbiome analysis revealed that first-degree relatives had significantly reduced microbiome diversity compared to their controls (observed species, P = 0.004; Chao1 index, P = 0.005), but this reduction was not observed in SLE patients.
31106972	1	83	theme	Systemic	166:173	arg1	SLE					196:198	SLE	196:198	SLE	196:198	OBJECTIVE Systemic lupus erythematosus (SLE) is characterized by the production of antibodies against self antigens.
31106972	1	83	theme	Systemic	166:173	arg1	erythematosus					181:193	OBJECTIVE Systemic lupus erythematosus	156:193	OBJECTIVE Systemic lupus erythematosus (SLE)	156:199	OBJECTIVE Systemic lupus erythematosus (SLE) is characterized by the production of antibodies against self antigens.
31106972	0	84	from	Link	2:5	arg1	Relatives					104:112	First-Degree Relatives	91:112	First-Degree Relatives of Systemic Lupus Erythematosus Patients	91:153	A Link Between Plasma Microbial Translocation, Microbiome, and Autoantibody Development in First-Degree Relatives of Systemic Lupus Erythematosus Patients.
31106972	10	85	theme	Padj	2007:2010	arg1	0.008					2014:2018	Padj = 0.008	2007:2018	Padj = 0.008	2007:2018	Bacteria in the Paenibacillus genus were the only overlapping differentially abundant bacteria in both cohorts, and were reduced in first-degree relatives (adjusted P [Padj ] = 2.13 × 10-12 ) and SLE patients (Padj = 0.008) but elevated in controls.
31106972	10	85	theme	Padj	2007:2010	arg1	patients					1997:2004	SLE patients	1993:2004	SLE patients (Padj = 0.008)	1993:2019	Bacteria in the Paenibacillus genus were the only overlapping differentially abundant bacteria in both cohorts, and were reduced in first-degree relatives (adjusted P [Padj ] = 2.13 × 10-12 ) and SLE patients (Padj = 0.008) but elevated in controls.
31106972	0	86	theme	First-Degree	91:102	arg1	Relatives					104:112	First-Degree Relatives	91:112	First-Degree Relatives of Systemic Lupus Erythematosus Patients	91:153	A Link Between Plasma Microbial Translocation, Microbiome, and Autoantibody Development in First-Degree Relatives of Systemic Lupus Erythematosus Patients.
31106972	4	87	theme	Limulus	775:781	arg1	assay					793:797	Limulus amebocyte assay	775:797	Limulus amebocyte assay	775:797	METHODS Plasma samples from 2 cohorts, one with 18 unrelated healthy controls and 18 first-degree relatives and the other with 19 healthy controls and 21 SLE patients, were assessed for autoantibody levels by autoantigen microarray analysis, measurement of lipopolysaccharide (LPS) levels by Limulus amebocyte assay, and determination of microbiome composition by microbial 16S ribosomal DNA sequencing.
31106972	4	88	theme	METHODS	483:489	arg1	samples					498:504	METHODS Plasma samples	483:504	METHODS Plasma samples from 2 cohorts, one with 18 unrelated healthy controls and 18 first-degree relatives and the other with 19 healthy controls and 21 SLE patients,	483:649	METHODS Plasma samples from 2 cohorts, one with 18 unrelated healthy controls and 18 first-degree relatives and the other with 19 healthy controls and 21 SLE patients, were assessed for autoantibody levels by autoantigen microarray analysis, measurement of lipopolysaccharide (LPS) levels by Limulus amebocyte assay, and determination of microbiome composition by microbial 16S ribosomal DNA sequencing.
31106972	8	89	theme	first-degree	1332:1343	arg1	relatives					1345:1353	first-degree relatives	1332:1353	first-degree relatives	1332:1353	Circulating microbiome analysis revealed that first-degree relatives had significantly reduced microbiome diversity compared to their controls (observed species, P = 0.004; Chao1 index, P = 0.005), but this reduction was not observed in SLE patients.
31106972	5	90	theme	control	1000:1006	arg1	groups					1008:1013	their control groups	994:1013	their control groups	994:1013	RESULTS First-degree relatives and SLE patients exhibited increased plasma autoantibody levels compared to their control groups.
31106972	9	91	theme	SLE	1783:1785	arg1	patients					1787:1794	SLE patients	1783:1794	SLE patients	1783:1794	The majority of bacteria that were differentially abundant between unrelated healthy controls and first-degree relatives were in the Firmicutes phylum, while differences in bacteria from several phyla were identified between healthy controls and SLE patients.
31106972	4	92	theme	levels	765:770	arg1	determination					804:816	determination	804:816	determination of microbiome composition by microbial 16S ribosomal DNA sequencing	804:884	METHODS Plasma samples from 2 cohorts, one with 18 unrelated healthy controls and 18 first-degree relatives and the other with 19 healthy controls and 21 SLE patients, were assessed for autoantibody levels by autoantigen microarray analysis, measurement of lipopolysaccharide (LPS) levels by Limulus amebocyte assay, and determination of microbiome composition by microbial 16S ribosomal DNA sequencing.
31106972	4	92	theme	levels	765:770	arg1	measurement					725:735	measurement	725:735	measurement of lipopolysaccharide (LPS) levels by Limulus amebocyte assay	725:797	METHODS Plasma samples from 2 cohorts, one with 18 unrelated healthy controls and 18 first-degree relatives and the other with 19 healthy controls and 21 SLE patients, were assessed for autoantibody levels by autoantigen microarray analysis, measurement of lipopolysaccharide (LPS) levels by Limulus amebocyte assay, and determination of microbiome composition by microbial 16S ribosomal DNA sequencing.
31106972	4	92	theme	levels	765:770	arg1	analysis					715:722	autoantigen microarray analysis	692:722	autoantigen microarray analysis	692:722	METHODS Plasma samples from 2 cohorts, one with 18 unrelated healthy controls and 18 first-degree relatives and the other with 19 healthy controls and 21 SLE patients, were assessed for autoantibody levels by autoantigen microarray analysis, measurement of lipopolysaccharide (LPS) levels by Limulus amebocyte assay, and determination of microbiome composition by microbial 16S ribosomal DNA sequencing.
31106972	7	93	theme	IgG	1202:1204	arg1	levels					1206:1211	plasma anti-double-stranded DNA IgG levels	1170:1211	plasma anti-double-stranded DNA IgG levels in first-degree relatives (r = 0.51, P = 0.03), but not in SLE patients	1170:1283	Plasma LPS levels positively correlated with plasma anti-double-stranded DNA IgG levels in first-degree relatives (r = 0.51, P = 0.03), but not in SLE patients.
31106972	5	94	theme	plasma	955:960	arg1	levels					975:980	increased plasma autoantibody levels	945:980	increased plasma autoantibody levels	945:980	RESULTS First-degree relatives and SLE patients exhibited increased plasma autoantibody levels compared to their control groups.
31106972	10	95	from	Bacteria	1797:1804	arg1	genus					1827:1831	the Paenibacillus genus	1809:1831	the Paenibacillus genus	1809:1831	Bacteria in the Paenibacillus genus were the only overlapping differentially abundant bacteria in both cohorts, and were reduced in first-degree relatives (adjusted P [Padj ] = 2.13 × 10-12 ) and SLE patients (Padj = 0.008) but elevated in controls.
31106972	11	96	theme	microbiome	2136:2145	arg1	composition					2147:2157	microbiome composition	2136:2157	microbiome composition	2136:2157	CONCLUSIONS These results indicate a possible role of plasma microbial translocation and microbiome composition in influencing autoantibody development in SLE.
31106972	8	97	theme	reduced	1373:1379	arg1	diversity					1392:1400	significantly reduced microbiome diversity	1359:1400	significantly reduced microbiome diversity	1359:1400	Circulating microbiome analysis revealed that first-degree relatives had significantly reduced microbiome diversity compared to their controls (observed species, P = 0.004; Chao1 index, P = 0.005), but this reduction was not observed in SLE patients.
31106972	3	98	theme	translocation	441:453	arg1	role					395:398	the role	391:398	the role of plasma autoantibody levels, microbial translocation, and the microbiome in SLE	391:480	This study was undertaken to investigate the role of plasma autoantibody levels, microbial translocation, and the microbiome in SLE.
31106972	0	99	theme	Plasma	15:20	arg1	Translocation					32:44	Plasma Microbial Translocation	15:44	Plasma Microbial Translocation	15:44	A Link Between Plasma Microbial Translocation, Microbiome, and Autoantibody Development in First-Degree Relatives of Systemic Lupus Erythematosus Patients.
31106972	11	100	theme	translocation	2118:2130	arg1	role					2093:2096	a possible role	2082:2096	a possible role of plasma microbial translocation and microbiome composition in influencing autoantibody development in SLE	2082:2204	CONCLUSIONS These results indicate a possible role of plasma microbial translocation and microbiome composition in influencing autoantibody development in SLE.
31106972	7	101	dep	=	1242:1242	arg1	0.51					1244:1247	0.51	1244:1247	0.51	1244:1247	Plasma LPS levels positively correlated with plasma anti-double-stranded DNA IgG levels in first-degree relatives (r = 0.51, P = 0.03), but not in SLE patients.
31106972	4	102	theme	lipopolysaccharide	740:757	arg1	levels					765:770	lipopolysaccharide (LPS) levels	740:770	lipopolysaccharide (LPS) levels	740:770	METHODS Plasma samples from 2 cohorts, one with 18 unrelated healthy controls and 18 first-degree relatives and the other with 19 healthy controls and 21 SLE patients, were assessed for autoantibody levels by autoantigen microarray analysis, measurement of lipopolysaccharide (LPS) levels by Limulus amebocyte assay, and determination of microbiome composition by microbial 16S ribosomal DNA sequencing.
31106972	9	103	theme	bacteria	1553:1560	arg1	majority					1541:1548	The majority	1537:1548	The majority of bacteria that were differentially abundant between unrelated healthy controls and first-degree relatives	1537:1656	The majority of bacteria that were differentially abundant between unrelated healthy controls and first-degree relatives were in the Firmicutes phylum, while differences in bacteria from several phyla were identified between healthy controls and SLE patients.
31106972	7	104	theme	SLE	1272:1274	arg1	patients					1276:1283	SLE patients	1272:1283	SLE patients	1272:1283	Plasma LPS levels positively correlated with plasma anti-double-stranded DNA IgG levels in first-degree relatives (r = 0.51, P = 0.03), but not in SLE patients.
31106972	11	105	theme	autoantibody	2174:2185	arg1	development					2187:2197	autoantibody development	2174:2197	autoantibody development	2174:2197	CONCLUSIONS These results indicate a possible role of plasma microbial translocation and microbiome composition in influencing autoantibody development in SLE.
31106972	10	106	theme	only	1842:1845	arg1	Bacteria					1797:1804	Bacteria	1797:1804	Bacteria in the Paenibacillus genus	1797:1831	Bacteria in the Paenibacillus genus were the only overlapping differentially abundant bacteria in both cohorts, and were reduced in first-degree relatives (adjusted P [Padj ] = 2.13 × 10-12 ) and SLE patients (Padj = 0.008) but elevated in controls.
31106972	10	106	theme	only	1842:1845	arg1	bacteria					1883:1890	the only overlapping differentially abundant bacteria	1838:1890	the only overlapping differentially abundant bacteria in both cohorts	1838:1906	Bacteria in the Paenibacillus genus were the only overlapping differentially abundant bacteria in both cohorts, and were reduced in first-degree relatives (adjusted P [Padj ] = 2.13 × 10-12 ) and SLE patients (Padj = 0.008) but elevated in controls.
31106972	3	107	theme	microbiome	464:473	arg1	role					395:398	the role	391:398	the role of plasma autoantibody levels, microbial translocation, and the microbiome in SLE	391:480	This study was undertaken to investigate the role of plasma autoantibody levels, microbial translocation, and the microbiome in SLE.
31106972	3	108	theme	autoantibody	410:421	arg1	levels					423:428	plasma autoantibody levels	403:428	plasma autoantibody levels	403:428	This study was undertaken to investigate the role of plasma autoantibody levels, microbial translocation, and the microbiome in SLE.
31106972	4	109	theme	unrelated	534:542	arg1	controls					552:559	18 unrelated healthy controls	531:559	18 unrelated healthy controls	531:559	METHODS Plasma samples from 2 cohorts, one with 18 unrelated healthy controls and 18 first-degree relatives and the other with 19 healthy controls and 21 SLE patients, were assessed for autoantibody levels by autoantigen microarray analysis, measurement of lipopolysaccharide (LPS) levels by Limulus amebocyte assay, and determination of microbiome composition by microbial 16S ribosomal DNA sequencing.
31106972	11	110	theme	possible	2084:2091	arg1	role					2093:2096	a possible role	2082:2096	a possible role of plasma microbial translocation and microbiome composition in influencing autoantibody development in SLE	2082:2204	CONCLUSIONS These results indicate a possible role of plasma microbial translocation and microbiome composition in influencing autoantibody development in SLE.
31106972	8	111	theme	observed	1430:1437	arg1	controls					1420:1427	their controls	1414:1427	their controls (observed species, P = 0.004; Chao1 index, P = 0.005)	1414:1481	Circulating microbiome analysis revealed that first-degree relatives had significantly reduced microbiome diversity compared to their controls (observed species, P = 0.004; Chao1 index, P = 0.005), but this reduction was not observed in SLE patients.
31106972	8	111	theme	observed	1430:1437	arg1	species					1439:1445	observed species	1430:1445	observed species	1430:1445	Circulating microbiome analysis revealed that first-degree relatives had significantly reduced microbiome diversity compared to their controls (observed species, P = 0.004; Chao1 index, P = 0.005), but this reduction was not observed in SLE patients.
31106972	9	112	theme	unrelated	1604:1612	arg1	controls					1622:1629	unrelated healthy controls	1604:1629	unrelated healthy controls	1604:1629	The majority of bacteria that were differentially abundant between unrelated healthy controls and first-degree relatives were in the Firmicutes phylum, while differences in bacteria from several phyla were identified between healthy controls and SLE patients.
31106972	4	113	theme	healthy	613:619	arg1	controls					621:628	19 healthy controls	610:628	19 healthy controls	610:628	METHODS Plasma samples from 2 cohorts, one with 18 unrelated healthy controls and 18 first-degree relatives and the other with 19 healthy controls and 21 SLE patients, were assessed for autoantibody levels by autoantigen microarray analysis, measurement of lipopolysaccharide (LPS) levels by Limulus amebocyte assay, and determination of microbiome composition by microbial 16S ribosomal DNA sequencing.
31106972	10	114	theme	2.13	1974:1977	arg1	relatives					1942:1950	first-degree relatives	1929:1950	first-degree relatives (adjusted P [Padj ] = 2.13 × 10-12 )	1929:1987	Bacteria in the Paenibacillus genus were the only overlapping differentially abundant bacteria in both cohorts, and were reduced in first-degree relatives (adjusted P [Padj ] = 2.13 × 10-12 ) and SLE patients (Padj = 0.008) but elevated in controls.
31106972	10	114	theme	2.13	1974:1977	arg1	×					1979:1979	adjusted P [Padj ] = 2.13 × 10-12	1953:1985	adjusted P [Padj ] = 2.13 × 10-12	1953:1985	Bacteria in the Paenibacillus genus were the only overlapping differentially abundant bacteria in both cohorts, and were reduced in first-degree relatives (adjusted P [Padj ] = 2.13 × 10-12 ) and SLE patients (Padj = 0.008) but elevated in controls.
31422193	4	0	theme	AgNPs	516:520	arg1	composites					504:513	silver nanoparticle (AgNP)/SCGS composites	472:513	silver nanoparticle (AgNP)/SCGS composites (AgNPs@SCGs)	472:526	This work describes the method for reusing the SCGs as biological macromolecular supports and reducing agents to prepare silver nanoparticle (AgNP)/SCGS composites (AgNPs@SCGs) by biogenic synthesis.
31422193	4	0	theme	AgNPs	516:520	arg1	SCGs					522:525	AgNPs@SCGs	516:525	AgNPs@SCGs	516:525	This work describes the method for reusing the SCGs as biological macromolecular supports and reducing agents to prepare silver nanoparticle (AgNP)/SCGS composites (AgNPs@SCGs) by biogenic synthesis.
31422193	6	1	theme	uniform	871:877	arg1	AgNPs					897:901	uniform and impurity-free AgNPs	871:901	uniform and impurity-free AgNPs on the surface of SCGs	871:924	Scanning electron microscopy (SEM) and X-ray diffractometer (XRD) analysis confirmed the reduction of silver ions to AgNPs, and showed that the pH 4.5 condition could generate uniform and impurity-free AgNPs on the surface of SCGs.
31422193	0	2	theme	green	81:85	arg1	grounds					29:35	coffee grounds	22:35	coffee grounds for silver nanoparticle immobilization	22:74	Polysaccharidic spent coffee grounds for silver nanoparticle immobilization as a green and highly efficient biocide.
31422193	0	2	theme	green	81:85	arg1	biocide					108:114	a green and highly efficient biocide	79:114	a green and highly efficient biocide	79:114	Polysaccharidic spent coffee grounds for silver nanoparticle immobilization as a green and highly efficient biocide.
31422193	6	3	theme	impurity-free	883:895	arg1	AgNPs					897:901	uniform and impurity-free AgNPs	871:901	uniform and impurity-free AgNPs on the surface of SCGs	871:924	Scanning electron microscopy (SEM) and X-ray diffractometer (XRD) analysis confirmed the reduction of silver ions to AgNPs, and showed that the pH 4.5 condition could generate uniform and impurity-free AgNPs on the surface of SCGs.
31422193	6	4	theme	ions	804:807	arg1	reduction					784:792	the reduction	780:792	the reduction of silver ions to AgNPs	780:816	Scanning electron microscopy (SEM) and X-ray diffractometer (XRD) analysis confirmed the reduction of silver ions to AgNPs, and showed that the pH 4.5 condition could generate uniform and impurity-free AgNPs on the surface of SCGs.
31422193	1	5	theme	abundant	153:160	arg1	polysaccharides					162:176	abundant polysaccharides	153:176	abundant polysaccharides	153:176	Spent coffee grounds (SCGs) contain abundant polysaccharides and several components with bioactivities.
31422193	4	6	dep	macromolecular	417:430	arg1	supports					432:439	supports	432:439	supports	432:439	This work describes the method for reusing the SCGs as biological macromolecular supports and reducing agents to prepare silver nanoparticle (AgNP)/SCGS composites (AgNPs@SCGs) by biogenic synthesis.
31422193	6	7	theme	pH 4.5	839:844	arg1	condition					846:854	the pH 4.5 condition	835:854	the pH 4.5 condition	835:854	Scanning electron microscopy (SEM) and X-ray diffractometer (XRD) analysis confirmed the reduction of silver ions to AgNPs, and showed that the pH 4.5 condition could generate uniform and impurity-free AgNPs on the surface of SCGs.
31422193	0	8	theme	efficient	98:106	arg1	grounds					29:35	coffee grounds	22:35	coffee grounds for silver nanoparticle immobilization	22:74	Polysaccharidic spent coffee grounds for silver nanoparticle immobilization as a green and highly efficient biocide.
31422193	0	8	theme	efficient	98:106	arg1	biocide					108:114	a green and highly efficient biocide	79:114	a green and highly efficient biocide	79:114	Polysaccharidic spent coffee grounds for silver nanoparticle immobilization as a green and highly efficient biocide.
31422193	5	9	theme	various	652:658	arg1	conditions					663:672	various pH conditions	652:672	various pH conditions at room temperature	652:692	The AgNPs anchored on the surface of SCGs were synthesized by mixing the SCGs in AgNO3 solution with various pH conditions at room temperature.
31422193	6	10	theme	silver	797:802	arg1	ions					804:807	silver ions	797:807	silver ions	797:807	Scanning electron microscopy (SEM) and X-ray diffractometer (XRD) analysis confirmed the reduction of silver ions to AgNPs, and showed that the pH 4.5 condition could generate uniform and impurity-free AgNPs on the surface of SCGs.
31422193	9	11	theme	green	1359:1363	arg1	method					1381:1386	the green and sustainable method	1355:1386	method	1381:1386	The transformation of the polysaccharidic SCGs to AgNPs@SCGs composites by the green and sustainable method makes them highly valuable for developing the applications on antimicrobial products.
31422193	7	12	theme	SCGs	1155:1158	arg1	nature					1141:1146	the original nature	1128:1146	the original nature of the SCGs	1128:1158	Fourier-transform infrared spectroscopy (FTIR), energy-dispersive X-ray spectroscopy (EDX), and thermal gravimetric analysis (TGA) showed that the reducing process of AgNPs was mild and could preserve the original nature of the SCGs.
31422193	4	13	theme	/SCGS	498:502	arg1	composites					504:513	silver nanoparticle (AgNP)/SCGS composites	472:513	silver nanoparticle (AgNP)/SCGS composites (AgNPs@SCGs)	472:526	This work describes the method for reusing the SCGs as biological macromolecular supports and reducing agents to prepare silver nanoparticle (AgNP)/SCGS composites (AgNPs@SCGs) by biogenic synthesis.
31422193	4	13	theme	/SCGS	498:502	arg1	SCGs					522:525	AgNPs@SCGs	516:525	AgNPs@SCGs	516:525	This work describes the method for reusing the SCGs as biological macromolecular supports and reducing agents to prepare silver nanoparticle (AgNP)/SCGS composites (AgNPs@SCGs) by biogenic synthesis.
31422193	5	14	theme	pH	660:661	arg1	conditions					663:672	various pH conditions	652:672	various pH conditions at room temperature	652:692	The AgNPs anchored on the surface of SCGs were synthesized by mixing the SCGs in AgNO3 solution with various pH conditions at room temperature.
31422193	5	15	theme	room	677:680	arg1	temperature					682:692	room temperature	677:692	room temperature	677:692	The AgNPs anchored on the surface of SCGs were synthesized by mixing the SCGs in AgNO3 solution with various pH conditions at room temperature.
31422193	9	16	theme	polysaccharidic	1306:1320	arg1	SCGs					1322:1325	the polysaccharidic SCGs	1302:1325	the polysaccharidic SCGs	1302:1325	The transformation of the polysaccharidic SCGs to AgNPs@SCGs composites by the green and sustainable method makes them highly valuable for developing the applications on antimicrobial products.
31422193	1	17	contain	contain	145:151	arg1	grounds					130:136	Spent coffee grounds	117:136	Spent coffee grounds (SCGs)	117:143	Spent coffee grounds (SCGs) contain abundant polysaccharides and several components with bioactivities.
31422193	1	17	contain	contain	145:151	arg2	polysaccharides					162:176	abundant polysaccharides	153:176	abundant polysaccharides	153:176	Spent coffee grounds (SCGs) contain abundant polysaccharides and several components with bioactivities.
31422193	1	17	contain	contain	145:151	arg2	components					190:199	several components	182:199	several components	182:199	Spent coffee grounds (SCGs) contain abundant polysaccharides and several components with bioactivities.
31422193	1	17	contain	contain	145:151	arg1	SCGs					139:142	SCGs	139:142	SCGs	139:142	Spent coffee grounds (SCGs) contain abundant polysaccharides and several components with bioactivities.
31422193	7	18	theme	Fourier-transform	927:943	arg1	FTIR					968:971	FTIR	968:971	FTIR	968:971	Fourier-transform infrared spectroscopy (FTIR), energy-dispersive X-ray spectroscopy (EDX), and thermal gravimetric analysis (TGA) showed that the reducing process of AgNPs was mild and could preserve the original nature of the SCGs.
31422193	7	18	theme	Fourier-transform	927:943	arg1	spectroscopy					954:965	Fourier-transform infrared spectroscopy	927:965	Fourier-transform infrared spectroscopy (FTIR)	927:972	Fourier-transform infrared spectroscopy (FTIR), energy-dispersive X-ray spectroscopy (EDX), and thermal gravimetric analysis (TGA) showed that the reducing process of AgNPs was mild and could preserve the original nature of the SCGs.
31422193	6	19	theme	electron	704:711	arg1	SEM					725:727	SEM	725:727	SEM	725:727	Scanning electron microscopy (SEM) and X-ray diffractometer (XRD) analysis confirmed the reduction of silver ions to AgNPs, and showed that the pH 4.5 condition could generate uniform and impurity-free AgNPs on the surface of SCGs.
31422193	6	19	theme	electron	704:711	arg1	microscopy					713:722	Scanning electron microscopy	695:722	Scanning electron microscopy (SEM)	695:728	Scanning electron microscopy (SEM) and X-ray diffractometer (XRD) analysis confirmed the reduction of silver ions to AgNPs, and showed that the pH 4.5 condition could generate uniform and impurity-free AgNPs on the surface of SCGs.
31422193	9	20	theme	sustainable	1369:1379	arg1	method					1381:1386	the green and sustainable method	1355:1386	method	1381:1386	The transformation of the polysaccharidic SCGs to AgNPs@SCGs composites by the green and sustainable method makes them highly valuable for developing the applications on antimicrobial products.
31422193	8	21	theme	AgNPs	1165:1169	arg1	composites					1176:1185	The AgNPs@SCGs composites	1161:1185	The AgNPs@SCGs composites	1161:1185	The AgNPs@SCGs composites exhibited an excellent antimicrobial ability against S. aureus and E. coli compared to SCGs.
31422193	9	22	theme	@	1335:1335	arg1	composites					1341:1350	AgNPs@SCGs composites	1330:1350	AgNPs@SCGs composites	1330:1350	The transformation of the polysaccharidic SCGs to AgNPs@SCGs composites by the green and sustainable method makes them highly valuable for developing the applications on antimicrobial products.
31422193	6	23	theme	Scanning	695:702	arg1	SEM					725:727	SEM	725:727	SEM	725:727	Scanning electron microscopy (SEM) and X-ray diffractometer (XRD) analysis confirmed the reduction of silver ions to AgNPs, and showed that the pH 4.5 condition could generate uniform and impurity-free AgNPs on the surface of SCGs.
31422193	6	23	theme	Scanning	695:702	arg1	microscopy					713:722	Scanning electron microscopy	695:722	Scanning electron microscopy (SEM)	695:728	Scanning electron microscopy (SEM) and X-ray diffractometer (XRD) analysis confirmed the reduction of silver ions to AgNPs, and showed that the pH 4.5 condition could generate uniform and impurity-free AgNPs on the surface of SCGs.
31422193	8	24	theme	SCGs	1171:1174	arg1	composites					1176:1185	The AgNPs@SCGs composites	1161:1185	The AgNPs@SCGs composites	1161:1185	The AgNPs@SCGs composites exhibited an excellent antimicrobial ability against S. aureus and E. coli compared to SCGs.
31422193	6	25	from	AgNPs	897:901	arg1	surface					910:916	the surface	906:916	the surface of SCGs	906:924	Scanning electron microscopy (SEM) and X-ray diffractometer (XRD) analysis confirmed the reduction of silver ions to AgNPs, and showed that the pH 4.5 condition could generate uniform and impurity-free AgNPs on the surface of SCGs.
31422193	1	26	theme	several	182:188	arg1	components					190:199	several components	182:199	several components	182:199	Spent coffee grounds (SCGs) contain abundant polysaccharides and several components with bioactivities.
31422193	7	27	theme	original	1132:1139	arg1	nature					1141:1146	the original nature	1128:1146	the original nature of the SCGs	1128:1158	Fourier-transform infrared spectroscopy (FTIR), energy-dispersive X-ray spectroscopy (EDX), and thermal gravimetric analysis (TGA) showed that the reducing process of AgNPs was mild and could preserve the original nature of the SCGs.
31422193	5	28	theme	AgNO3	632:636	arg1	solution					638:645	AgNO3 solution	632:645	AgNO3 solution	632:645	The AgNPs anchored on the surface of SCGs were synthesized by mixing the SCGs in AgNO3 solution with various pH conditions at room temperature.
31422193	8	29	dep	exhibited	1187:1195	arg1	compared					1262:1269	compared	1262:1269	compared to SCGs	1262:1277	The AgNPs@SCGs composites exhibited an excellent antimicrobial ability against S. aureus and E. coli compared to SCGs.
31422193	5	30	theme	SCGs	588:591	arg1	surface					577:583	the surface	573:583	the surface of SCGs	573:591	The AgNPs anchored on the surface of SCGs were synthesized by mixing the SCGs in AgNO3 solution with various pH conditions at room temperature.
31422193	7	31	theme	infrared	945:952	arg1	FTIR					968:971	FTIR	968:971	FTIR	968:971	Fourier-transform infrared spectroscopy (FTIR), energy-dispersive X-ray spectroscopy (EDX), and thermal gravimetric analysis (TGA) showed that the reducing process of AgNPs was mild and could preserve the original nature of the SCGs.
31422193	7	31	theme	infrared	945:952	arg1	spectroscopy					954:965	Fourier-transform infrared spectroscopy	927:965	Fourier-transform infrared spectroscopy (FTIR)	927:972	Fourier-transform infrared spectroscopy (FTIR), energy-dispersive X-ray spectroscopy (EDX), and thermal gravimetric analysis (TGA) showed that the reducing process of AgNPs was mild and could preserve the original nature of the SCGs.
31422193	8	32	theme	antimicrobial	1210:1222	arg1	ability					1224:1230	an excellent antimicrobial ability	1197:1230	an excellent antimicrobial ability	1197:1230	The AgNPs@SCGs composites exhibited an excellent antimicrobial ability against S. aureus and E. coli compared to SCGs.
31422193	0	33	theme	coffee	22:27	arg1	grounds					29:35	coffee grounds	22:35	coffee grounds for silver nanoparticle immobilization	22:74	Polysaccharidic spent coffee grounds for silver nanoparticle immobilization as a green and highly efficient biocide.
31422193	0	33	theme	coffee	22:27	arg1	biocide					108:114	a green and highly efficient biocide	79:114	a green and highly efficient biocide	79:114	Polysaccharidic spent coffee grounds for silver nanoparticle immobilization as a green and highly efficient biocide.
31422193	7	34	theme	energy-dispersive	975:991	arg1	EDX					1013:1015	EDX	1013:1015	EDX	1013:1015	Fourier-transform infrared spectroscopy (FTIR), energy-dispersive X-ray spectroscopy (EDX), and thermal gravimetric analysis (TGA) showed that the reducing process of AgNPs was mild and could preserve the original nature of the SCGs.
31422193	7	34	theme	energy-dispersive	975:991	arg1	spectroscopy					999:1010	energy-dispersive X-ray spectroscopy	975:1010	energy-dispersive X-ray spectroscopy (EDX)	975:1016	Fourier-transform infrared spectroscopy (FTIR), energy-dispersive X-ray spectroscopy (EDX), and thermal gravimetric analysis (TGA) showed that the reducing process of AgNPs was mild and could preserve the original nature of the SCGs.
31422193	4	35	theme	nanoparticle	479:490	arg1	composites					504:513	silver nanoparticle (AgNP)/SCGS composites	472:513	silver nanoparticle (AgNP)/SCGS composites (AgNPs@SCGs)	472:526	This work describes the method for reusing the SCGs as biological macromolecular supports and reducing agents to prepare silver nanoparticle (AgNP)/SCGS composites (AgNPs@SCGs) by biogenic synthesis.
31422193	4	35	theme	nanoparticle	479:490	arg1	SCGs					522:525	AgNPs@SCGs	516:525	AgNPs@SCGs	516:525	This work describes the method for reusing the SCGs as biological macromolecular supports and reducing agents to prepare silver nanoparticle (AgNP)/SCGS composites (AgNPs@SCGs) by biogenic synthesis.
31422193	8	36	theme	excellent	1200:1208	arg1	ability					1224:1230	an excellent antimicrobial ability	1197:1230	an excellent antimicrobial ability	1197:1230	The AgNPs@SCGs composites exhibited an excellent antimicrobial ability against S. aureus and E. coli compared to SCGs.
31422193	0	37	theme	silver	41:46	arg1	immobilization					61:74	silver nanoparticle immobilization	41:74	silver nanoparticle immobilization	41:74	Polysaccharidic spent coffee grounds for silver nanoparticle immobilization as a green and highly efficient biocide.
31422193	5	38	from	SCGs	624:627	arg1	solution					638:645	AgNO3 solution	632:645	AgNO3 solution	632:645	The AgNPs anchored on the surface of SCGs were synthesized by mixing the SCGs in AgNO3 solution with various pH conditions at room temperature.
31422193	7	39	theme	thermal	1023:1029	arg1	TGA					1053:1055	TGA	1053:1055	TGA	1053:1055	Fourier-transform infrared spectroscopy (FTIR), energy-dispersive X-ray spectroscopy (EDX), and thermal gravimetric analysis (TGA) showed that the reducing process of AgNPs was mild and could preserve the original nature of the SCGs.
31422193	7	39	theme	thermal	1023:1029	arg1	analysis					1043:1050	thermal gravimetric analysis	1023:1050	thermal gravimetric analysis (TGA)	1023:1056	Fourier-transform infrared spectroscopy (FTIR), energy-dispersive X-ray spectroscopy (EDX), and thermal gravimetric analysis (TGA) showed that the reducing process of AgNPs was mild and could preserve the original nature of the SCGs.
31422193	4	40	theme	silver	472:477	arg1	composites					504:513	silver nanoparticle (AgNP)/SCGS composites	472:513	silver nanoparticle (AgNP)/SCGS composites (AgNPs@SCGs)	472:526	This work describes the method for reusing the SCGs as biological macromolecular supports and reducing agents to prepare silver nanoparticle (AgNP)/SCGS composites (AgNPs@SCGs) by biogenic synthesis.
31422193	4	40	theme	silver	472:477	arg1	SCGs					522:525	AgNPs@SCGs	516:525	AgNPs@SCGs	516:525	This work describes the method for reusing the SCGs as biological macromolecular supports and reducing agents to prepare silver nanoparticle (AgNP)/SCGS composites (AgNPs@SCGs) by biogenic synthesis.
31422193	4	41	theme	biogenic	531:538	arg1	synthesis					540:548	biogenic synthesis	531:548	biogenic synthesis	531:548	This work describes the method for reusing the SCGs as biological macromolecular supports and reducing agents to prepare silver nanoparticle (AgNP)/SCGS composites (AgNPs@SCGs) by biogenic synthesis.
31422193	7	42	theme	gravimetric	1031:1041	arg1	TGA					1053:1055	TGA	1053:1055	TGA	1053:1055	Fourier-transform infrared spectroscopy (FTIR), energy-dispersive X-ray spectroscopy (EDX), and thermal gravimetric analysis (TGA) showed that the reducing process of AgNPs was mild and could preserve the original nature of the SCGs.
31422193	7	42	theme	gravimetric	1031:1041	arg1	analysis					1043:1050	thermal gravimetric analysis	1023:1050	thermal gravimetric analysis (TGA)	1023:1056	Fourier-transform infrared spectroscopy (FTIR), energy-dispersive X-ray spectroscopy (EDX), and thermal gravimetric analysis (TGA) showed that the reducing process of AgNPs was mild and could preserve the original nature of the SCGs.
31422193	4	43	theme	AgNP	493:496	arg1	composites					504:513	silver nanoparticle (AgNP)/SCGS composites	472:513	silver nanoparticle (AgNP)/SCGS composites (AgNPs@SCGs)	472:526	This work describes the method for reusing the SCGs as biological macromolecular supports and reducing agents to prepare silver nanoparticle (AgNP)/SCGS composites (AgNPs@SCGs) by biogenic synthesis.
31422193	4	43	theme	AgNP	493:496	arg1	SCGs					522:525	AgNPs@SCGs	516:525	AgNPs@SCGs	516:525	This work describes the method for reusing the SCGs as biological macromolecular supports and reducing agents to prepare silver nanoparticle (AgNP)/SCGS composites (AgNPs@SCGs) by biogenic synthesis.
31422193	6	44	theme	XRD	756:758	arg1	analysis					761:768	X-ray diffractometer (XRD) analysis	734:768	X-ray diffractometer (XRD) analysis	734:768	Scanning electron microscopy (SEM) and X-ray diffractometer (XRD) analysis confirmed the reduction of silver ions to AgNPs, and showed that the pH 4.5 condition could generate uniform and impurity-free AgNPs on the surface of SCGs.
31422193	7	45	theme	AgNPs	1094:1098	arg1	mild					1104:1107	mild	1104:1107	mild	1104:1107	Fourier-transform infrared spectroscopy (FTIR), energy-dispersive X-ray spectroscopy (EDX), and thermal gravimetric analysis (TGA) showed that the reducing process of AgNPs was mild and could preserve the original nature of the SCGs.
31422193	7	45	theme	AgNPs	1094:1098	arg1	process					1083:1089	the reducing process	1070:1089	the reducing process of AgNPs	1070:1098	Fourier-transform infrared spectroscopy (FTIR), energy-dispersive X-ray spectroscopy (EDX), and thermal gravimetric analysis (TGA) showed that the reducing process of AgNPs was mild and could preserve the original nature of the SCGs.
31422193	6	46	theme	SCGs	921:924	arg1	surface					910:916	the surface	906:916	the surface of SCGs	906:924	Scanning electron microscopy (SEM) and X-ray diffractometer (XRD) analysis confirmed the reduction of silver ions to AgNPs, and showed that the pH 4.5 condition could generate uniform and impurity-free AgNPs on the surface of SCGs.
31422193	3	47	theme	SCGs	321:324	arg1	Modifications					304:316	Modifications	304:316	Modifications of SCGs	304:324	Modifications of SCGs may overcome this issue.
31422193	1	48	theme	Spent	117:121	arg1	grounds					130:136	Spent coffee grounds	117:136	Spent coffee grounds (SCGs)	117:143	Spent coffee grounds (SCGs) contain abundant polysaccharides and several components with bioactivities.
31422193	1	48	theme	Spent	117:121	arg1	SCGs					139:142	SCGs	139:142	SCGs	139:142	Spent coffee grounds (SCGs) contain abundant polysaccharides and several components with bioactivities.
31422193	0	49	theme	nanoparticle	48:59	arg1	immobilization					61:74	silver nanoparticle immobilization	41:74	silver nanoparticle immobilization	41:74	Polysaccharidic spent coffee grounds for silver nanoparticle immobilization as a green and highly efficient biocide.
31422193	9	50	theme	SCGs	1322:1325	arg1	transformation					1284:1297	The transformation	1280:1297	The transformation of the polysaccharidic SCGs to AgNPs@SCGs composites by the green and sustainable method	1280:1386	The transformation of the polysaccharidic SCGs to AgNPs@SCGs composites by the green and sustainable method makes them highly valuable for developing the applications on antimicrobial products.
31422193	1	51	with	components	190:199	arg1	bioactivities					206:218	bioactivities	206:218	bioactivities	206:218	Spent coffee grounds (SCGs) contain abundant polysaccharides and several components with bioactivities.
31422193	2	52	theme	many	229:232	arg1	bio-functionalities					234:252	many bio-functionalities	229:252	many bio-functionalities	229:252	Despite many bio-functionalities, their bioactivities are not always satisfactory.
31422193	6	53	theme	diffractometer	740:753	arg1	analysis					761:768	X-ray diffractometer (XRD) analysis	734:768	X-ray diffractometer (XRD) analysis	734:768	Scanning electron microscopy (SEM) and X-ray diffractometer (XRD) analysis confirmed the reduction of silver ions to AgNPs, and showed that the pH 4.5 condition could generate uniform and impurity-free AgNPs on the surface of SCGs.
31422193	1	54	theme	coffee	123:128	arg1	grounds					130:136	Spent coffee grounds	117:136	Spent coffee grounds (SCGs)	117:143	Spent coffee grounds (SCGs) contain abundant polysaccharides and several components with bioactivities.
31422193	1	54	theme	coffee	123:128	arg1	SCGs					139:142	SCGs	139:142	SCGs	139:142	Spent coffee grounds (SCGs) contain abundant polysaccharides and several components with bioactivities.
31422193	9	55	from	applications	1434:1445	arg1	products					1464:1471	antimicrobial products	1450:1471	antimicrobial products	1450:1471	The transformation of the polysaccharidic SCGs to AgNPs@SCGs composites by the green and sustainable method makes them highly valuable for developing the applications on antimicrobial products.
31422193	1	56	with	polysaccharides	162:176	arg1	bioactivities					206:218	bioactivities	206:218	bioactivities	206:218	Spent coffee grounds (SCGs) contain abundant polysaccharides and several components with bioactivities.
31422193	4	57	theme	reducing	445:452	arg1	agents					454:459	reducing agents	445:459	reducing agents	445:459	This work describes the method for reusing the SCGs as biological macromolecular supports and reducing agents to prepare silver nanoparticle (AgNP)/SCGS composites (AgNPs@SCGs) by biogenic synthesis.
31422193	6	58	theme	X-ray	734:738	arg1	analysis					761:768	X-ray diffractometer (XRD) analysis	734:768	X-ray diffractometer (XRD) analysis	734:768	Scanning electron microscopy (SEM) and X-ray diffractometer (XRD) analysis confirmed the reduction of silver ions to AgNPs, and showed that the pH 4.5 condition could generate uniform and impurity-free AgNPs on the surface of SCGs.
31422193	9	59	theme	AgNPs	1330:1334	arg1	composites					1341:1350	AgNPs@SCGs composites	1330:1350	AgNPs@SCGs composites	1330:1350	The transformation of the polysaccharidic SCGs to AgNPs@SCGs composites by the green and sustainable method makes them highly valuable for developing the applications on antimicrobial products.
31422193	7	60	theme	X-ray	993:997	arg1	EDX					1013:1015	EDX	1013:1015	EDX	1013:1015	Fourier-transform infrared spectroscopy (FTIR), energy-dispersive X-ray spectroscopy (EDX), and thermal gravimetric analysis (TGA) showed that the reducing process of AgNPs was mild and could preserve the original nature of the SCGs.
31422193	7	60	theme	X-ray	993:997	arg1	spectroscopy					999:1010	energy-dispersive X-ray spectroscopy	975:1010	energy-dispersive X-ray spectroscopy (EDX)	975:1016	Fourier-transform infrared spectroscopy (FTIR), energy-dispersive X-ray spectroscopy (EDX), and thermal gravimetric analysis (TGA) showed that the reducing process of AgNPs was mild and could preserve the original nature of the SCGs.
31422193	9	61	theme	antimicrobial	1450:1462	arg1	products					1464:1471	antimicrobial products	1450:1471	antimicrobial products	1450:1471	The transformation of the polysaccharidic SCGs to AgNPs@SCGs composites by the green and sustainable method makes them highly valuable for developing the applications on antimicrobial products.
31422193	5	62	from	temperature	682:692	arg1	conditions					663:672	various pH conditions	652:672	various pH conditions at room temperature	652:692	The AgNPs anchored on the surface of SCGs were synthesized by mixing the SCGs in AgNO3 solution with various pH conditions at room temperature.
31422193	9	63	theme	SCGs	1336:1339	arg1	composites					1341:1350	AgNPs@SCGs composites	1330:1350	AgNPs@SCGs composites	1330:1350	The transformation of the polysaccharidic SCGs to AgNPs@SCGs composites by the green and sustainable method makes them highly valuable for developing the applications on antimicrobial products.
31422193	8	64	theme	@	1170:1170	arg1	composites					1176:1185	The AgNPs@SCGs composites	1161:1185	The AgNPs@SCGs composites	1161:1185	The AgNPs@SCGs composites exhibited an excellent antimicrobial ability against S. aureus and E. coli compared to SCGs.
31422193	4	65	theme	@	521:521	arg1	composites					504:513	silver nanoparticle (AgNP)/SCGS composites	472:513	silver nanoparticle (AgNP)/SCGS composites (AgNPs@SCGs)	472:526	This work describes the method for reusing the SCGs as biological macromolecular supports and reducing agents to prepare silver nanoparticle (AgNP)/SCGS composites (AgNPs@SCGs) by biogenic synthesis.
31422193	4	65	theme	@	521:521	arg1	SCGs					522:525	AgNPs@SCGs	516:525	AgNPs@SCGs	516:525	This work describes the method for reusing the SCGs as biological macromolecular supports and reducing agents to prepare silver nanoparticle (AgNP)/SCGS composites (AgNPs@SCGs) by biogenic synthesis.
31422193	7	66	theme	reducing	1074:1081	arg1	mild					1104:1107	mild	1104:1107	mild	1104:1107	Fourier-transform infrared spectroscopy (FTIR), energy-dispersive X-ray spectroscopy (EDX), and thermal gravimetric analysis (TGA) showed that the reducing process of AgNPs was mild and could preserve the original nature of the SCGs.
31422193	7	66	theme	reducing	1074:1081	arg1	process					1083:1089	the reducing process	1070:1089	the reducing process of AgNPs	1070:1098	Fourier-transform infrared spectroscopy (FTIR), energy-dispersive X-ray spectroscopy (EDX), and thermal gravimetric analysis (TGA) showed that the reducing process of AgNPs was mild and could preserve the original nature of the SCGs.
31733576	3	0	theme	phylum	673:678	arg1	Bacteroidetes					680:692	the phylum Bacteroidetes	669:692	the phylum Bacteroidetes	669:692	In vivo studies revealed that the administrations of LBP to mice resulted in an increase in the abundance of the phyla Proteobacteria and Firmicutes, while reducing the ratio of the phylum Bacteroidetes.
31733576	3	1	theme	In	491:492	arg1	studies					499:505	In vivo studies	491:505	In vivo studies	491:505	In vivo studies revealed that the administrations of LBP to mice resulted in an increase in the abundance of the phyla Proteobacteria and Firmicutes, while reducing the ratio of the phylum Bacteroidetes.
31733576	0	2	theme	polysaccharide	87:100	arg1	effects					60:66	Prebiotic, immuno-stimulating and gut microbiota-modulating effects	0:66	Prebiotic, immuno-stimulating and gut microbiota-modulating effects of Lycium barbarum polysaccharide.	0:101	Prebiotic, immuno-stimulating and gut microbiota-modulating effects of Lycium barbarum polysaccharide.
31733576	1	3	theme	Lycium	166:171	arg1	LBP					198:200	LBP	198:200	LBP	198:200	The present study was done to evaluate the prebiotic effect of Lycium barbarum polysaccharide (LBP), its effect on murine fecal microbiota composition and innate immune response.
31733576	1	3	theme	Lycium	166:171	arg1	polysaccharide					182:195	Lycium barbarum polysaccharide	166:195	Lycium barbarum polysaccharide (LBP)	166:201	The present study was done to evaluate the prebiotic effect of Lycium barbarum polysaccharide (LBP), its effect on murine fecal microbiota composition and innate immune response.
31733576	2	4	theme	bacteria	349:356	arg1	growth					319:324	the growth	315:324	the growth of selective probiotic bacteria	315:356	Results showed that LBP supports the growth of selective probiotic bacteria with a maximum of 8.23 (log10 cfu/mL) and 6.62 (log10 cfu/mL) for Lactobacillus acidophilus and Bifidobacterium longum respectively.
31733576	5	5	from	TGF-β	873:877	arg1	sIgA					901:904	sIgA	901:904	sIgA	901:904	The concentrations of TGF-β and IL-6 in serum and sIgA in the colon content were enriched significantly after LBP administrations in mice.
31733576	5	5	from	TGF-β	873:877	arg1	serum					891:895	serum	891:895	serum	891:895	The concentrations of TGF-β and IL-6 in serum and sIgA in the colon content were enriched significantly after LBP administrations in mice.
31733576	5	6	theme	colon	913:917	arg1	content					919:925	the colon content	909:925	the colon content	909:925	The concentrations of TGF-β and IL-6 in serum and sIgA in the colon content were enriched significantly after LBP administrations in mice.
31733576	6	7	theme	control	1098:1104	arg1	P < 0.05					1113:1120	P < 0.05	1113:1120	P < 0.05	1113:1120	The thymus index and spleen index of mice treated with LBP displayed significant difference compared to the control group (P < 0.05).
31733576	6	7	theme	control	1098:1104	arg1	group					1106:1110	the control group	1094:1110	the control group (P < 0.05)	1094:1121	The thymus index and spleen index of mice treated with LBP displayed significant difference compared to the control group (P < 0.05).
31733576	1	8	theme	innate	258:263	arg1	response					272:279	innate immune response	258:279	innate immune response	258:279	The present study was done to evaluate the prebiotic effect of Lycium barbarum polysaccharide (LBP), its effect on murine fecal microbiota composition and innate immune response.
31733576	7	9	theme	potential	1178:1186	arg1	prebiotic					1188:1196	a potential prebiotic	1176:1196	a potential prebiotic	1176:1196	These findings suggest that LBP is a good source as a potential prebiotic and can enhance the intestinal microbiota and boost beneficial bacteria levels, modulate innate immune response.
31733576	1	10	theme	immune	265:270	arg1	response					272:279	innate immune response	258:279	innate immune response	258:279	The present study was done to evaluate the prebiotic effect of Lycium barbarum polysaccharide (LBP), its effect on murine fecal microbiota composition and innate immune response.
31733576	1	11	from	effect	156:161	arg1	composition					242:252	murine fecal microbiota composition	218:252	murine fecal microbiota composition	218:252	The present study was done to evaluate the prebiotic effect of Lycium barbarum polysaccharide (LBP), its effect on murine fecal microbiota composition and innate immune response.
31733576	5	12	from	administrations	965:979	arg1	mice					984:987	mice	984:987	mice	984:987	The concentrations of TGF-β and IL-6 in serum and sIgA in the colon content were enriched significantly after LBP administrations in mice.
31733576	1	13	theme	barbarum	173:180	arg1	LBP					198:200	LBP	198:200	LBP	198:200	The present study was done to evaluate the prebiotic effect of Lycium barbarum polysaccharide (LBP), its effect on murine fecal microbiota composition and innate immune response.
31733576	1	13	theme	barbarum	173:180	arg1	polysaccharide					182:195	Lycium barbarum polysaccharide	166:195	Lycium barbarum polysaccharide (LBP)	166:201	The present study was done to evaluate the prebiotic effect of Lycium barbarum polysaccharide (LBP), its effect on murine fecal microbiota composition and innate immune response.
31733576	3	14	theme	Bacteroidetes	680:692	arg1	ratio					660:664	the ratio	656:664	the ratio of the phylum Bacteroidetes	656:692	In vivo studies revealed that the administrations of LBP to mice resulted in an increase in the abundance of the phyla Proteobacteria and Firmicutes, while reducing the ratio of the phylum Bacteroidetes.
31733576	7	15	theme	beneficial	1250:1259	arg1	bacteria					1261:1268	beneficial bacteria	1250:1268	beneficial bacteria levels	1250:1275	These findings suggest that LBP is a good source as a potential prebiotic and can enhance the intestinal microbiota and boost beneficial bacteria levels, modulate innate immune response.
31733576	4	16	theme	LBP	737:739	arg1	administration					719:732	the administration	715:732	the administration of LBP	715:739	At the genus level, the administration of LBP stimulated the emergence of some potential probiotic genera (Akkermansia, Lactobacillus, and Prevotellaceae).
31733576	1	17	theme	polysaccharide	182:195	arg1	effect					156:161	the prebiotic effect	142:161	the prebiotic effect of Lycium barbarum polysaccharide (LBP)	142:201	The present study was done to evaluate the prebiotic effect of Lycium barbarum polysaccharide (LBP), its effect on murine fecal microbiota composition and innate immune response.
31733576	1	17	theme	polysaccharide	182:195	arg1	response					272:279	innate immune response	258:279	innate immune response	258:279	The present study was done to evaluate the prebiotic effect of Lycium barbarum polysaccharide (LBP), its effect on murine fecal microbiota composition and innate immune response.
31733576	1	17	theme	polysaccharide	182:195	arg1	effect					208:213	its effect	204:213	its effect on murine fecal microbiota composition	204:252	The present study was done to evaluate the prebiotic effect of Lycium barbarum polysaccharide (LBP), its effect on murine fecal microbiota composition and innate immune response.
31733576	7	18	theme	good	1161:1164	arg1	LBP					1152:1154	LBP	1152:1154	LBP	1152:1154	These findings suggest that LBP is a good source as a potential prebiotic and can enhance the intestinal microbiota and boost beneficial bacteria levels, modulate innate immune response.
31733576	7	18	theme	good	1161:1164	arg1	source					1166:1171	a good source	1159:1171	a good source	1159:1171	These findings suggest that LBP is a good source as a potential prebiotic and can enhance the intestinal microbiota and boost beneficial bacteria levels, modulate innate immune response.
31733576	1	19	from	response	272:279	arg1	composition					242:252	murine fecal microbiota composition	218:252	murine fecal microbiota composition	218:252	The present study was done to evaluate the prebiotic effect of Lycium barbarum polysaccharide (LBP), its effect on murine fecal microbiota composition and innate immune response.
31733576	5	20	from	serum	891:895	arg1	concentrations					855:868	The concentrations	851:868	The concentrations of TGF-β and IL-6 in serum and sIgA in the colon content	851:925	The concentrations of TGF-β and IL-6 in serum and sIgA in the colon content were enriched significantly after LBP administrations in mice.
31733576	3	21	dep	In	491:492	arg1	vivo					494:497	vivo	494:497	vivo	494:497	In vivo studies revealed that the administrations of LBP to mice resulted in an increase in the abundance of the phyla Proteobacteria and Firmicutes, while reducing the ratio of the phylum Bacteroidetes.
31733576	2	22	theme	8.23	376:379	arg1	maximum					365:371	a maximum	363:371	a maximum of 8.23 (log10 cfu/mL) and 6.62 (log10 cfu/mL) for Lactobacillus acidophilus and Bifidobacterium longum respectively	363:488	Results showed that LBP supports the growth of selective probiotic bacteria with a maximum of 8.23 (log10 cfu/mL) and 6.62 (log10 cfu/mL) for Lactobacillus acidophilus and Bifidobacterium longum respectively.
31733576	0	23	theme	Prebiotic	0:8	arg1	effects					60:66	Prebiotic, immuno-stimulating and gut microbiota-modulating effects	0:66	Prebiotic, immuno-stimulating and gut microbiota-modulating effects of Lycium barbarum polysaccharide.	0:101	Prebiotic, immuno-stimulating and gut microbiota-modulating effects of Lycium barbarum polysaccharide.
31733576	1	24	theme	present	107:113	arg1	study					115:119	The present study	103:119	The present study	103:119	The present study was done to evaluate the prebiotic effect of Lycium barbarum polysaccharide (LBP), its effect on murine fecal microbiota composition and innate immune response.
31733576	5	25	theme	TGF-β	873:877	arg1	concentrations					855:868	The concentrations	851:868	The concentrations of TGF-β and IL-6 in serum and sIgA in the colon content	851:925	The concentrations of TGF-β and IL-6 in serum and sIgA in the colon content were enriched significantly after LBP administrations in mice.
31733576	2	26	theme	log10	406:410	arg1	cfu/mL					412:417	log10 cfu/mL	406:417	log10 cfu/mL	406:417	Results showed that LBP supports the growth of selective probiotic bacteria with a maximum of 8.23 (log10 cfu/mL) and 6.62 (log10 cfu/mL) for Lactobacillus acidophilus and Bifidobacterium longum respectively.
31733576	2	26	theme	log10	406:410	arg1	6.62					400:403	6.62	400:403	6.62	400:403	Results showed that LBP supports the growth of selective probiotic bacteria with a maximum of 8.23 (log10 cfu/mL) and 6.62 (log10 cfu/mL) for Lactobacillus acidophilus and Bifidobacterium longum respectively.
31733576	4	27	theme	genus	702:706	arg1	level					708:712	the genus level	698:712	the genus level	698:712	At the genus level, the administration of LBP stimulated the emergence of some potential probiotic genera (Akkermansia, Lactobacillus, and Prevotellaceae).
31733576	5	28	from	concentrations	855:868	arg1	content					919:925	the colon content	909:925	the colon content	909:925	The concentrations of TGF-β and IL-6 in serum and sIgA in the colon content were enriched significantly after LBP administrations in mice.
31733576	5	28	from	concentrations	855:868	arg1	sIgA					901:904	sIgA	901:904	sIgA	901:904	The concentrations of TGF-β and IL-6 in serum and sIgA in the colon content were enriched significantly after LBP administrations in mice.
31733576	5	28	from	concentrations	855:868	arg1	serum					891:895	serum	891:895	serum	891:895	The concentrations of TGF-β and IL-6 in serum and sIgA in the colon content were enriched significantly after LBP administrations in mice.
31733576	3	29	theme	LBP	544:546	arg1	administrations					525:539	the administrations	521:539	the administrations of LBP to mice	521:554	In vivo studies revealed that the administrations of LBP to mice resulted in an increase in the abundance of the phyla Proteobacteria and Firmicutes, while reducing the ratio of the phylum Bacteroidetes.
31733576	6	30	theme	mice	1027:1030	arg1	index					1018:1022	The thymus index and spleen index	990:1022	The thymus index and spleen index of mice treated with LBP	990:1047	The thymus index and spleen index of mice treated with LBP displayed significant difference compared to the control group (P < 0.05).
31733576	2	31	theme	6.62	400:403	arg1	maximum					365:371	a maximum	363:371	a maximum of 8.23 (log10 cfu/mL) and 6.62 (log10 cfu/mL) for Lactobacillus acidophilus and Bifidobacterium longum respectively	363:488	Results showed that LBP supports the growth of selective probiotic bacteria with a maximum of 8.23 (log10 cfu/mL) and 6.62 (log10 cfu/mL) for Lactobacillus acidophilus and Bifidobacterium longum respectively.
31733576	0	32	theme	immuno-stimulating	11:28	arg1	effects					60:66	Prebiotic, immuno-stimulating and gut microbiota-modulating effects	0:66	Prebiotic, immuno-stimulating and gut microbiota-modulating effects of Lycium barbarum polysaccharide.	0:101	Prebiotic, immuno-stimulating and gut microbiota-modulating effects of Lycium barbarum polysaccharide.
31733576	0	33	dep	Lycium	71:76	arg1	barbarum					78:85	barbarum	78:85	barbarum	78:85	Prebiotic, immuno-stimulating and gut microbiota-modulating effects of Lycium barbarum polysaccharide.
31733576	3	34	from	increase	571:578	arg1	abundance					587:595	the abundance	583:595	the abundance of the phyla Proteobacteria and Firmicutes	583:638	In vivo studies revealed that the administrations of LBP to mice resulted in an increase in the abundance of the phyla Proteobacteria and Firmicutes, while reducing the ratio of the phylum Bacteroidetes.
31733576	4	35	dep	genera	794:799	arg1	Lactobacillus					815:827	Lactobacillus	815:827	Lactobacillus	815:827	At the genus level, the administration of LBP stimulated the emergence of some potential probiotic genera (Akkermansia, Lactobacillus, and Prevotellaceae).
31733576	4	35	dep	genera	794:799	arg1	Prevotellaceae					834:847	Prevotellaceae	834:847	Prevotellaceae	834:847	At the genus level, the administration of LBP stimulated the emergence of some potential probiotic genera (Akkermansia, Lactobacillus, and Prevotellaceae).
31733576	4	35	dep	genera	794:799	arg1	Akkermansia					802:812	Akkermansia	802:812	Akkermansia	802:812	At the genus level, the administration of LBP stimulated the emergence of some potential probiotic genera (Akkermansia, Lactobacillus, and Prevotellaceae).
31733576	0	36	theme	microbiota-modulating	38:58	arg1	effects					60:66	Prebiotic, immuno-stimulating and gut microbiota-modulating effects	0:66	Prebiotic, immuno-stimulating and gut microbiota-modulating effects of Lycium barbarum polysaccharide.	0:101	Prebiotic, immuno-stimulating and gut microbiota-modulating effects of Lycium barbarum polysaccharide.
31733576	5	37	theme	IL-6	883:886	arg1	concentrations					855:868	The concentrations	851:868	The concentrations of TGF-β and IL-6 in serum and sIgA in the colon content	851:925	The concentrations of TGF-β and IL-6 in serum and sIgA in the colon content were enriched significantly after LBP administrations in mice.
31733576	4	38	theme	probiotic	784:792	arg1	genera					794:799	some potential probiotic genera	769:799	some potential probiotic genera (Akkermansia, Lactobacillus, and Prevotellaceae)	769:848	At the genus level, the administration of LBP stimulated the emergence of some potential probiotic genera (Akkermansia, Lactobacillus, and Prevotellaceae).
31733576	6	39	theme	index	1001:1005	arg1	index					1018:1022	The thymus index and spleen index	990:1022	The thymus index and spleen index of mice treated with LBP	990:1047	The thymus index and spleen index of mice treated with LBP displayed significant difference compared to the control group (P < 0.05).
31733576	0	40	theme	gut	34:36	arg1	effects					60:66	Prebiotic, immuno-stimulating and gut microbiota-modulating effects	0:66	Prebiotic, immuno-stimulating and gut microbiota-modulating effects of Lycium barbarum polysaccharide.	0:101	Prebiotic, immuno-stimulating and gut microbiota-modulating effects of Lycium barbarum polysaccharide.
31733576	3	41	theme	phyla	604:608	arg1	abundance					587:595	the abundance	583:595	the abundance of the phyla Proteobacteria and Firmicutes	583:638	In vivo studies revealed that the administrations of LBP to mice resulted in an increase in the abundance of the phyla Proteobacteria and Firmicutes, while reducing the ratio of the phylum Bacteroidetes.
31733576	4	42	theme	potential	774:782	arg1	genera					794:799	some potential probiotic genera	769:799	some potential probiotic genera (Akkermansia, Lactobacillus, and Prevotellaceae)	769:848	At the genus level, the administration of LBP stimulated the emergence of some potential probiotic genera (Akkermansia, Lactobacillus, and Prevotellaceae).
31733576	6	43	theme	thymus	994:999	arg1	index					1001:1005	thymus index	994:1005	thymus index	994:1005	The thymus index and spleen index of mice treated with LBP displayed significant difference compared to the control group (P < 0.05).
31733576	7	44	theme	innate	1287:1292	arg1	response					1301:1308	innate immune response	1287:1308	innate immune response	1287:1308	These findings suggest that LBP is a good source as a potential prebiotic and can enhance the intestinal microbiota and boost beneficial bacteria levels, modulate innate immune response.
31733576	1	45	from	effect	208:213	arg1	composition					242:252	murine fecal microbiota composition	218:252	murine fecal microbiota composition	218:252	The present study was done to evaluate the prebiotic effect of Lycium barbarum polysaccharide (LBP), its effect on murine fecal microbiota composition and innate immune response.
31733576	2	46	theme	probiotic	339:347	arg1	bacteria					349:356	selective probiotic bacteria	329:356	selective probiotic bacteria	329:356	Results showed that LBP supports the growth of selective probiotic bacteria with a maximum of 8.23 (log10 cfu/mL) and 6.62 (log10 cfu/mL) for Lactobacillus acidophilus and Bifidobacterium longum respectively.
31733576	2	47	theme	log10	382:386	arg1	8.23					376:379	8.23	376:379	8.23	376:379	Results showed that LBP supports the growth of selective probiotic bacteria with a maximum of 8.23 (log10 cfu/mL) and 6.62 (log10 cfu/mL) for Lactobacillus acidophilus and Bifidobacterium longum respectively.
31733576	2	47	theme	log10	382:386	arg1	cfu/mL					388:393	log10 cfu/mL	382:393	log10 cfu/mL	382:393	Results showed that LBP supports the growth of selective probiotic bacteria with a maximum of 8.23 (log10 cfu/mL) and 6.62 (log10 cfu/mL) for Lactobacillus acidophilus and Bifidobacterium longum respectively.
31733576	4	48	theme	genera	794:799	arg1	emergence					756:764	the emergence	752:764	the emergence of some potential probiotic genera (Akkermansia, Lactobacillus, and Prevotellaceae)	752:848	At the genus level, the administration of LBP stimulated the emergence of some potential probiotic genera (Akkermansia, Lactobacillus, and Prevotellaceae).
31733576	2	49	theme	selective	329:337	arg1	bacteria					349:356	selective probiotic bacteria	329:356	selective probiotic bacteria	329:356	Results showed that LBP supports the growth of selective probiotic bacteria with a maximum of 8.23 (log10 cfu/mL) and 6.62 (log10 cfu/mL) for Lactobacillus acidophilus and Bifidobacterium longum respectively.
31733576	5	50	from	IL-6	883:886	arg1	sIgA					901:904	sIgA	901:904	sIgA	901:904	The concentrations of TGF-β and IL-6 in serum and sIgA in the colon content were enriched significantly after LBP administrations in mice.
31733576	5	50	from	IL-6	883:886	arg1	serum					891:895	serum	891:895	serum	891:895	The concentrations of TGF-β and IL-6 in serum and sIgA in the colon content were enriched significantly after LBP administrations in mice.
31733576	3	51	dep	phyla	604:608	arg1	phyla					604:608	the phyla Proteobacteria and Firmicutes	600:638	the phyla Proteobacteria and Firmicutes	600:638	In vivo studies revealed that the administrations of LBP to mice resulted in an increase in the abundance of the phyla Proteobacteria and Firmicutes, while reducing the ratio of the phylum Bacteroidetes.
31733576	3	51	dep	phyla	604:608	arg1	Firmicutes					629:638	Firmicutes	629:638	Firmicutes	629:638	In vivo studies revealed that the administrations of LBP to mice resulted in an increase in the abundance of the phyla Proteobacteria and Firmicutes, while reducing the ratio of the phylum Bacteroidetes.
31733576	3	51	dep	phyla	604:608	arg1	Proteobacteria					610:623	Proteobacteria	610:623	Proteobacteria	610:623	In vivo studies revealed that the administrations of LBP to mice resulted in an increase in the abundance of the phyla Proteobacteria and Firmicutes, while reducing the ratio of the phylum Bacteroidetes.
31733576	7	52	theme	intestinal	1218:1227	arg1	microbiota					1229:1238	the intestinal microbiota	1214:1238	the intestinal microbiota	1214:1238	These findings suggest that LBP is a good source as a potential prebiotic and can enhance the intestinal microbiota and boost beneficial bacteria levels, modulate innate immune response.
31733576	6	53	theme	significant	1059:1069	arg1	difference					1071:1080	significant difference	1059:1080	significant difference	1059:1080	The thymus index and spleen index of mice treated with LBP displayed significant difference compared to the control group (P < 0.05).
31733576	7	54	theme	bacteria	1261:1268	arg1	levels					1270:1275	beneficial bacteria levels	1250:1275	beneficial bacteria levels	1250:1275	These findings suggest that LBP is a good source as a potential prebiotic and can enhance the intestinal microbiota and boost beneficial bacteria levels, modulate innate immune response.
31733576	1	55	theme	murine	218:223	arg1	composition					242:252	murine fecal microbiota composition	218:252	murine fecal microbiota composition	218:252	The present study was done to evaluate the prebiotic effect of Lycium barbarum polysaccharide (LBP), its effect on murine fecal microbiota composition and innate immune response.
31733576	5	56	theme	LBP	961:963	arg1	administrations					965:979	LBP administrations	961:979	LBP administrations in mice	961:987	The concentrations of TGF-β and IL-6 in serum and sIgA in the colon content were enriched significantly after LBP administrations in mice.
31733576	6	57	theme	spleen	1011:1016	arg1	index					1018:1022	The thymus index and spleen index	990:1022	The thymus index and spleen index of mice treated with LBP	990:1047	The thymus index and spleen index of mice treated with LBP displayed significant difference compared to the control group (P < 0.05).
31733576	1	58	theme	prebiotic	146:154	arg1	effect					156:161	the prebiotic effect	142:161	the prebiotic effect of Lycium barbarum polysaccharide (LBP)	142:201	The present study was done to evaluate the prebiotic effect of Lycium barbarum polysaccharide (LBP), its effect on murine fecal microbiota composition and innate immune response.
31733576	5	59	from	sIgA	901:904	arg1	concentrations					855:868	The concentrations	851:868	The concentrations of TGF-β and IL-6 in serum and sIgA in the colon content	851:925	The concentrations of TGF-β and IL-6 in serum and sIgA in the colon content were enriched significantly after LBP administrations in mice.
31733576	1	60	theme	fecal	225:229	arg1	composition					242:252	murine fecal microbiota composition	218:252	murine fecal microbiota composition	218:252	The present study was done to evaluate the prebiotic effect of Lycium barbarum polysaccharide (LBP), its effect on murine fecal microbiota composition and innate immune response.
31733576	0	61	theme	Lycium	71:76	arg1	polysaccharide					87:100	Lycium barbarum polysaccharide	71:100	Lycium barbarum polysaccharide	71:100	Prebiotic, immuno-stimulating and gut microbiota-modulating effects of Lycium barbarum polysaccharide.
31733576	7	62	theme	immune	1294:1299	arg1	response					1301:1308	innate immune response	1287:1308	innate immune response	1287:1308	These findings suggest that LBP is a good source as a potential prebiotic and can enhance the intestinal microbiota and boost beneficial bacteria levels, modulate innate immune response.
31733576	1	63	theme	microbiota	231:240	arg1	composition					242:252	murine fecal microbiota composition	218:252	murine fecal microbiota composition	218:252	The present study was done to evaluate the prebiotic effect of Lycium barbarum polysaccharide (LBP), its effect on murine fecal microbiota composition and innate immune response.
30168793	2	0	theme	strain	111:116	arg1	XZHG99T					118:124	strain XZHG99T	111:124	strain XZHG99T	111:124	A novel actinomycete, designated strain XZHG99T, was isolated from soil taken from colour desert, Dengpa District, Tibet Autonomous Region, China.
30168793	4	1	theme	G+C	814:816	arg1	content					818:824	DNA G+C content	810:824	DNA G+C content of 69.9 mol%	810:837	The strain showed morphological and chemotaxonomic features typical of the genus Streptomyces: branched substrate and aerial mycelia, straight spore chains and smooth spores; ll-diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H8), MK-9(H2), MK-9(H6) and MK-9(H10) as menaquinones; diphosphatidylglycerol, phospatidylethanolamine, phosphatidylinositol, phosphotidylinositol mannoside and phosphatidylglycerol as prominent phospholipids; iso-C16 : 0, anteiso-C15 : 0 and C16 : 0 as major cellular fatty acids; and DNA G+C content of 69.9 mol%.
30168793	6	2	theme	Streptomyces	1249:1260	arg1	species					1262:1268	phylogenetically related Streptomyces species	1224:1268	phylogenetically related Streptomyces species	1224:1268	Based on comparison of phenotypic properties and the low level of DNA-DNA relatedness, strain XZHG99T can be distinguished from phylogenetically related Streptomyces species and is suggested to represent a novel species of the genus Streptomyces, for which the name Streptomyces dengpaensis sp.
30168793	4	3	theme	cell-wall	494:502	arg1	peptidoglycan					504:516	the cell-wall peptidoglycan	490:516	the cell-wall peptidoglycan	490:516	The strain showed morphological and chemotaxonomic features typical of the genus Streptomyces: branched substrate and aerial mycelia, straight spore chains and smooth spores; ll-diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H8), MK-9(H2), MK-9(H6) and MK-9(H10) as menaquinones; diphosphatidylglycerol, phospatidylethanolamine, phosphatidylinositol, phosphotidylinositol mannoside and phosphatidylglycerol as prominent phospholipids; iso-C16 : 0, anteiso-C15 : 0 and C16 : 0 as major cellular fatty acids; and DNA G+C content of 69.9 mol%.
30168793	4	4	theme	fatty	793:797	arg1	acids					799:803	major cellular fatty acids	778:803	major cellular fatty acids	778:803	The strain showed morphological and chemotaxonomic features typical of the genus Streptomyces: branched substrate and aerial mycelia, straight spore chains and smooth spores; ll-diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H8), MK-9(H2), MK-9(H6) and MK-9(H10) as menaquinones; diphosphatidylglycerol, phospatidylethanolamine, phosphatidylinositol, phosphotidylinositol mannoside and phosphatidylglycerol as prominent phospholipids; iso-C16 : 0, anteiso-C15 : 0 and C16 : 0 as major cellular fatty acids; and DNA G+C content of 69.9 mol%.
30168793	5	5	theme	Streptomyces	974:985	arg1	KC-035T					1003:1009	Streptomyces krungchingensis KC-035T	974:1009	Streptomyces krungchingensis KC-035T (98.14 %)	974:1019	16S rRNA gene sequence analysis indicated that strain XZHG99T showed high similarity to Streptomyces albiflavescensm20T (98.42 %) and Streptomyces krungchingensis KC-035T (98.14 %) as well as formed a monophyletic clade with them in the phylogenetic tree.
30168793	5	5	theme	Streptomyces	974:985	arg1	%					1018:1018	98.14 %	1012:1018	98.14 %	1012:1018	16S rRNA gene sequence analysis indicated that strain XZHG99T showed high similarity to Streptomyces albiflavescensm20T (98.42 %) and Streptomyces krungchingensis KC-035T (98.14 %) as well as formed a monophyletic clade with them in the phylogenetic tree.
30168793	6	6	theme	Streptomyces	1362:1373	arg1	sp					1387:1388	the name Streptomyces dengpaensis sp	1353:1388	the name Streptomyces dengpaensis sp	1353:1388	Based on comparison of phenotypic properties and the low level of DNA-DNA relatedness, strain XZHG99T can be distinguished from phylogenetically related Streptomyces species and is suggested to represent a novel species of the genus Streptomyces, for which the name Streptomyces dengpaensis sp.
30168793	4	7	theme	spore	432:436	arg1	chains					438:443	straight spore chains	423:443	straight spore chains	423:443	The strain showed morphological and chemotaxonomic features typical of the genus Streptomyces: branched substrate and aerial mycelia, straight spore chains and smooth spores; ll-diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H8), MK-9(H2), MK-9(H6) and MK-9(H10) as menaquinones; diphosphatidylglycerol, phospatidylethanolamine, phosphatidylinositol, phosphotidylinositol mannoside and phosphatidylglycerol as prominent phospholipids; iso-C16 : 0, anteiso-C15 : 0 and C16 : 0 as major cellular fatty acids; and DNA G+C content of 69.9 mol%.
30168793	5	8	theme	monophyletic	1041:1052	arg1	clade					1054:1058	a monophyletic clade	1039:1058	a monophyletic clade with them	1039:1068	16S rRNA gene sequence analysis indicated that strain XZHG99T showed high similarity to Streptomyces albiflavescensm20T (98.42 %) and Streptomyces krungchingensis KC-035T (98.14 %) as well as formed a monophyletic clade with them in the phylogenetic tree.
30168793	4	9	theme	Streptomyces	370:381	arg1	typical					349:355	typical	349:355	typical	349:355	The strain showed morphological and chemotaxonomic features typical of the genus Streptomyces: branched substrate and aerial mycelia, straight spore chains and smooth spores; ll-diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H8), MK-9(H2), MK-9(H6) and MK-9(H10) as menaquinones; diphosphatidylglycerol, phospatidylethanolamine, phosphatidylinositol, phosphotidylinositol mannoside and phosphatidylglycerol as prominent phospholipids; iso-C16 : 0, anteiso-C15 : 0 and C16 : 0 as major cellular fatty acids; and DNA G+C content of 69.9 mol%.
30168793	8	10	theme	type	1413:1416	arg1	XZHG99T					1428:1434	XZHG99T	1428:1434	XZHG99T (=CGMCC 4.7472T=KCTC 49090T)	1428:1463	The type strain is XZHG99T (=CGMCC 4.7472T=KCTC 49090T).
30168793	8	10	theme	type	1413:1416	arg1	strain					1418:1423	The type strain	1409:1423	The type strain	1409:1423	The type strain is XZHG99T (=CGMCC 4.7472T=KCTC 49090T).
30168793	4	11	dep	Streptomyces	370:381	arg1	H8					524:525	H8	524:525	H8	524:525	The strain showed morphological and chemotaxonomic features typical of the genus Streptomyces: branched substrate and aerial mycelia, straight spore chains and smooth spores; ll-diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H8), MK-9(H2), MK-9(H6) and MK-9(H10) as menaquinones; diphosphatidylglycerol, phospatidylethanolamine, phosphatidylinositol, phosphotidylinositol mannoside and phosphatidylglycerol as prominent phospholipids; iso-C16 : 0, anteiso-C15 : 0 and C16 : 0 as major cellular fatty acids; and DNA G+C content of 69.9 mol%.
30168793	4	11	dep	Streptomyces	370:381	arg1	mycelia					414:420	aerial mycelia	407:420	aerial mycelia	407:420	The strain showed morphological and chemotaxonomic features typical of the genus Streptomyces: branched substrate and aerial mycelia, straight spore chains and smooth spores; ll-diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H8), MK-9(H2), MK-9(H6) and MK-9(H10) as menaquinones; diphosphatidylglycerol, phospatidylethanolamine, phosphatidylinositol, phosphotidylinositol mannoside and phosphatidylglycerol as prominent phospholipids; iso-C16 : 0, anteiso-C15 : 0 and C16 : 0 as major cellular fatty acids; and DNA G+C content of 69.9 mol%.
30168793	4	11	dep	Streptomyces	370:381	arg1	spores					456:461	smooth spores	449:461	smooth spores	449:461	The strain showed morphological and chemotaxonomic features typical of the genus Streptomyces: branched substrate and aerial mycelia, straight spore chains and smooth spores; ll-diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H8), MK-9(H2), MK-9(H6) and MK-9(H10) as menaquinones; diphosphatidylglycerol, phospatidylethanolamine, phosphatidylinositol, phosphotidylinositol mannoside and phosphatidylglycerol as prominent phospholipids; iso-C16 : 0, anteiso-C15 : 0 and C16 : 0 as major cellular fatty acids; and DNA G+C content of 69.9 mol%.
30168793	4	11	dep	Streptomyces	370:381	arg1	substrate					393:401	branched substrate	384:401	branched substrate	384:401	The strain showed morphological and chemotaxonomic features typical of the genus Streptomyces: branched substrate and aerial mycelia, straight spore chains and smooth spores; ll-diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H8), MK-9(H2), MK-9(H6) and MK-9(H10) as menaquinones; diphosphatidylglycerol, phospatidylethanolamine, phosphatidylinositol, phosphotidylinositol mannoside and phosphatidylglycerol as prominent phospholipids; iso-C16 : 0, anteiso-C15 : 0 and C16 : 0 as major cellular fatty acids; and DNA G+C content of 69.9 mol%.
30168793	4	11	dep	Streptomyces	370:381	arg1	MK-9					519:522	MK-9	519:522	MK-9	519:522	The strain showed morphological and chemotaxonomic features typical of the genus Streptomyces: branched substrate and aerial mycelia, straight spore chains and smooth spores; ll-diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H8), MK-9(H2), MK-9(H6) and MK-9(H10) as menaquinones; diphosphatidylglycerol, phospatidylethanolamine, phosphatidylinositol, phosphotidylinositol mannoside and phosphatidylglycerol as prominent phospholipids; iso-C16 : 0, anteiso-C15 : 0 and C16 : 0 as major cellular fatty acids; and DNA G+C content of 69.9 mol%.
30168793	4	11	dep	Streptomyces	370:381	arg1	chains					438:443	straight spore chains	423:443	straight spore chains	423:443	The strain showed morphological and chemotaxonomic features typical of the genus Streptomyces: branched substrate and aerial mycelia, straight spore chains and smooth spores; ll-diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H8), MK-9(H2), MK-9(H6) and MK-9(H10) as menaquinones; diphosphatidylglycerol, phospatidylethanolamine, phosphatidylinositol, phosphotidylinositol mannoside and phosphatidylglycerol as prominent phospholipids; iso-C16 : 0, anteiso-C15 : 0 and C16 : 0 as major cellular fatty acids; and DNA G+C content of 69.9 mol%.
30168793	4	11	dep	Streptomyces	370:381	arg1	acid					482:485	ll-diaminopimelic acid	464:485	ll-diaminopimelic acid in the cell-wall peptidoglycan	464:516	The strain showed morphological and chemotaxonomic features typical of the genus Streptomyces: branched substrate and aerial mycelia, straight spore chains and smooth spores; ll-diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H8), MK-9(H2), MK-9(H6) and MK-9(H10) as menaquinones; diphosphatidylglycerol, phospatidylethanolamine, phosphatidylinositol, phosphotidylinositol mannoside and phosphatidylglycerol as prominent phospholipids; iso-C16 : 0, anteiso-C15 : 0 and C16 : 0 as major cellular fatty acids; and DNA G+C content of 69.9 mol%.
30168793	6	12	theme	dengpaensis	1375:1385	arg1	sp					1387:1388	the name Streptomyces dengpaensis sp	1353:1388	the name Streptomyces dengpaensis sp	1353:1388	Based on comparison of phenotypic properties and the low level of DNA-DNA relatedness, strain XZHG99T can be distinguished from phylogenetically related Streptomyces species and is suggested to represent a novel species of the genus Streptomyces, for which the name Streptomyces dengpaensis sp.
30168793	4	13	from	acid	482:485	arg1	peptidoglycan					504:516	the cell-wall peptidoglycan	490:516	the cell-wall peptidoglycan	490:516	The strain showed morphological and chemotaxonomic features typical of the genus Streptomyces: branched substrate and aerial mycelia, straight spore chains and smooth spores; ll-diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H8), MK-9(H2), MK-9(H6) and MK-9(H10) as menaquinones; diphosphatidylglycerol, phospatidylethanolamine, phosphatidylinositol, phosphotidylinositol mannoside and phosphatidylglycerol as prominent phospholipids; iso-C16 : 0, anteiso-C15 : 0 and C16 : 0 as major cellular fatty acids; and DNA G+C content of 69.9 mol%.
30168793	5	14	with	clade	1054:1058	arg1	them					1065:1068	them	1065:1068	them	1065:1068	16S rRNA gene sequence analysis indicated that strain XZHG99T showed high similarity to Streptomyces albiflavescensm20T (98.42 %) and Streptomyces krungchingensis KC-035T (98.14 %) as well as formed a monophyletic clade with them in the phylogenetic tree.
30168793	5	15	theme	16S	840:842	arg1	analysis					863:870	16S rRNA gene sequence analysis	840:870	16S rRNA gene sequence analysis	840:870	16S rRNA gene sequence analysis indicated that strain XZHG99T showed high similarity to Streptomyces albiflavescensm20T (98.42 %) and Streptomyces krungchingensis KC-035T (98.14 %) as well as formed a monophyletic clade with them in the phylogenetic tree.
30168793	4	16	theme	smooth	449:454	arg1	spores					456:461	smooth spores	449:461	smooth spores	449:461	The strain showed morphological and chemotaxonomic features typical of the genus Streptomyces: branched substrate and aerial mycelia, straight spore chains and smooth spores; ll-diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H8), MK-9(H2), MK-9(H6) and MK-9(H10) as menaquinones; diphosphatidylglycerol, phospatidylethanolamine, phosphatidylinositol, phosphotidylinositol mannoside and phosphatidylglycerol as prominent phospholipids; iso-C16 : 0, anteiso-C15 : 0 and C16 : 0 as major cellular fatty acids; and DNA G+C content of 69.9 mol%.
30168793	6	17	theme	DNA-DNA	1162:1168	arg1	relatedness					1170:1180	DNA-DNA relatedness	1162:1180	DNA-DNA relatedness	1162:1180	Based on comparison of phenotypic properties and the low level of DNA-DNA relatedness, strain XZHG99T can be distinguished from phylogenetically related Streptomyces species and is suggested to represent a novel species of the genus Streptomyces, for which the name Streptomyces dengpaensis sp.
30168793	4	18	theme	branched	384:391	arg1	substrate					393:401	branched substrate	384:401	branched substrate	384:401	The strain showed morphological and chemotaxonomic features typical of the genus Streptomyces: branched substrate and aerial mycelia, straight spore chains and smooth spores; ll-diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H8), MK-9(H2), MK-9(H6) and MK-9(H10) as menaquinones; diphosphatidylglycerol, phospatidylethanolamine, phosphatidylinositol, phosphotidylinositol mannoside and phosphatidylglycerol as prominent phospholipids; iso-C16 : 0, anteiso-C15 : 0 and C16 : 0 as major cellular fatty acids; and DNA G+C content of 69.9 mol%.
30168793	1	19	theme	desert	65:70	arg1	soil					72:75	desert soil	65:75	desert soil	65:75	nov., an actinomycete isolated from desert soil.
30168793	6	20	theme	low	1149:1151	arg1	level					1153:1157	the low level	1145:1157	the low level of DNA-DNA relatedness	1145:1180	Based on comparison of phenotypic properties and the low level of DNA-DNA relatedness, strain XZHG99T can be distinguished from phylogenetically related Streptomyces species and is suggested to represent a novel species of the genus Streptomyces, for which the name Streptomyces dengpaensis sp.
30168793	4	21	theme	ll-diaminopimelic	464:480	arg1	acid					482:485	ll-diaminopimelic acid	464:485	ll-diaminopimelic acid in the cell-wall peptidoglycan	464:516	The strain showed morphological and chemotaxonomic features typical of the genus Streptomyces: branched substrate and aerial mycelia, straight spore chains and smooth spores; ll-diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H8), MK-9(H2), MK-9(H6) and MK-9(H10) as menaquinones; diphosphatidylglycerol, phospatidylethanolamine, phosphatidylinositol, phosphotidylinositol mannoside and phosphatidylglycerol as prominent phospholipids; iso-C16 : 0, anteiso-C15 : 0 and C16 : 0 as major cellular fatty acids; and DNA G+C content of 69.9 mol%.
30168793	8	22	theme	4.7472T=KCTC	1444:1455	arg1	XZHG99T					1428:1434	XZHG99T	1428:1434	XZHG99T (=CGMCC 4.7472T=KCTC 49090T)	1428:1463	The type strain is XZHG99T (=CGMCC 4.7472T=KCTC 49090T).
30168793	8	22	theme	4.7472T=KCTC	1444:1455	arg1	49090T					1457:1462	=CGMCC 4.7472T=KCTC 49090T	1437:1462	=CGMCC 4.7472T=KCTC 49090T	1437:1462	The type strain is XZHG99T (=CGMCC 4.7472T=KCTC 49090T).
30168793	0	23	theme	dengpaensis	13:23	arg1	sp					25:26	Streptomyces dengpaensis sp	0:26	Streptomyces dengpaensis sp.	0:27	Streptomyces dengpaensis sp.
30168793	4	24	theme	cellular	784:791	arg1	acids					799:803	major cellular fatty acids	778:803	major cellular fatty acids	778:803	The strain showed morphological and chemotaxonomic features typical of the genus Streptomyces: branched substrate and aerial mycelia, straight spore chains and smooth spores; ll-diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H8), MK-9(H2), MK-9(H6) and MK-9(H10) as menaquinones; diphosphatidylglycerol, phospatidylethanolamine, phosphatidylinositol, phosphotidylinositol mannoside and phosphatidylglycerol as prominent phospholipids; iso-C16 : 0, anteiso-C15 : 0 and C16 : 0 as major cellular fatty acids; and DNA G+C content of 69.9 mol%.
30168793	4	25	theme	typical	349:355	arg1	features					340:347	morphological and chemotaxonomic features	307:347	morphological and chemotaxonomic features typical of the genus Streptomyces: branched substrate and aerial mycelia, straight spore chains and smooth spores; ll-diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H8), MK-9(H2), MK-9(H6) and MK-9(H10) as menaquinones; diphosphatidylglycerol, phospatidylethanolamine, phosphatidylinositol, phosphotidylinositol mannoside and phosphatidylglycerol as prominent phospholipids; iso-C16 : 0, anteiso-C15 	307:758	The strain showed morphological and chemotaxonomic features typical of the genus Streptomyces: branched substrate and aerial mycelia, straight spore chains and smooth spores; ll-diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H8), MK-9(H2), MK-9(H6) and MK-9(H10) as menaquinones; diphosphatidylglycerol, phospatidylethanolamine, phosphatidylinositol, phosphotidylinositol mannoside and phosphatidylglycerol as prominent phospholipids; iso-C16 : 0, anteiso-C15 : 0 and C16 : 0 as major cellular fatty acids; and DNA G+C content of 69.9 mol%.
30168793	5	26	theme	rRNA	844:847	arg1	analysis					863:870	16S rRNA gene sequence analysis	840:870	16S rRNA gene sequence analysis	840:870	16S rRNA gene sequence analysis indicated that strain XZHG99T showed high similarity to Streptomyces albiflavescensm20T (98.42 %) and Streptomyces krungchingensis KC-035T (98.14 %) as well as formed a monophyletic clade with them in the phylogenetic tree.
30168793	0	27	theme	Streptomyces	0:11	arg1	sp					25:26	Streptomyces dengpaensis sp	0:26	Streptomyces dengpaensis sp.	0:27	Streptomyces dengpaensis sp.
30168793	4	28	theme	major	778:782	arg1	acids					799:803	major cellular fatty acids	778:803	major cellular fatty acids	778:803	The strain showed morphological and chemotaxonomic features typical of the genus Streptomyces: branched substrate and aerial mycelia, straight spore chains and smooth spores; ll-diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H8), MK-9(H2), MK-9(H6) and MK-9(H10) as menaquinones; diphosphatidylglycerol, phospatidylethanolamine, phosphatidylinositol, phosphotidylinositol mannoside and phosphatidylglycerol as prominent phospholipids; iso-C16 : 0, anteiso-C15 : 0 and C16 : 0 as major cellular fatty acids; and DNA G+C content of 69.9 mol%.
30168793	4	29	theme	genus	364:368	arg1	Streptomyces					370:381	the genus Streptomyces	360:381	the genus Streptomyces	360:381	The strain showed morphological and chemotaxonomic features typical of the genus Streptomyces: branched substrate and aerial mycelia, straight spore chains and smooth spores; ll-diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H8), MK-9(H2), MK-9(H6) and MK-9(H10) as menaquinones; diphosphatidylglycerol, phospatidylethanolamine, phosphatidylinositol, phosphotidylinositol mannoside and phosphatidylglycerol as prominent phospholipids; iso-C16 : 0, anteiso-C15 : 0 and C16 : 0 as major cellular fatty acids; and DNA G+C content of 69.9 mol%.
30168793	4	29	theme	genus	364:368	arg1	mannoside					671:679	phosphotidylinositol mannoside	650:679	phosphotidylinositol mannoside	650:679	The strain showed morphological and chemotaxonomic features typical of the genus Streptomyces: branched substrate and aerial mycelia, straight spore chains and smooth spores; ll-diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H8), MK-9(H2), MK-9(H6) and MK-9(H10) as menaquinones; diphosphatidylglycerol, phospatidylethanolamine, phosphatidylinositol, phosphotidylinositol mannoside and phosphatidylglycerol as prominent phospholipids; iso-C16 : 0, anteiso-C15 : 0 and C16 : 0 as major cellular fatty acids; and DNA G+C content of 69.9 mol%.
30168793	4	29	theme	genus	364:368	arg1	H10					557:559	H10	557:559	H10	557:559	The strain showed morphological and chemotaxonomic features typical of the genus Streptomyces: branched substrate and aerial mycelia, straight spore chains and smooth spores; ll-diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H8), MK-9(H2), MK-9(H6) and MK-9(H10) as menaquinones; diphosphatidylglycerol, phospatidylethanolamine, phosphatidylinositol, phosphotidylinositol mannoside and phosphatidylglycerol as prominent phospholipids; iso-C16 : 0, anteiso-C15 : 0 and C16 : 0 as major cellular fatty acids; and DNA G+C content of 69.9 mol%.
30168793	4	29	theme	genus	364:368	arg1	MK-9					539:542	MK-9	539:542	MK-9	539:542	The strain showed morphological and chemotaxonomic features typical of the genus Streptomyces: branched substrate and aerial mycelia, straight spore chains and smooth spores; ll-diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H8), MK-9(H2), MK-9(H6) and MK-9(H10) as menaquinones; diphosphatidylglycerol, phospatidylethanolamine, phosphatidylinositol, phosphotidylinositol mannoside and phosphatidylglycerol as prominent phospholipids; iso-C16 : 0, anteiso-C15 : 0 and C16 : 0 as major cellular fatty acids; and DNA G+C content of 69.9 mol%.
30168793	4	29	theme	genus	364:368	arg1	MK-9					529:532	MK-9	529:532	MK-9	529:532	The strain showed morphological and chemotaxonomic features typical of the genus Streptomyces: branched substrate and aerial mycelia, straight spore chains and smooth spores; ll-diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H8), MK-9(H2), MK-9(H6) and MK-9(H10) as menaquinones; diphosphatidylglycerol, phospatidylethanolamine, phosphatidylinositol, phosphotidylinositol mannoside and phosphatidylglycerol as prominent phospholipids; iso-C16 : 0, anteiso-C15 : 0 and C16 : 0 as major cellular fatty acids; and DNA G+C content of 69.9 mol%.
30168793	4	29	theme	genus	364:368	arg1	phospatidylethanolamine					603:625	phospatidylethanolamine	603:625	phospatidylethanolamine	603:625	The strain showed morphological and chemotaxonomic features typical of the genus Streptomyces: branched substrate and aerial mycelia, straight spore chains and smooth spores; ll-diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H8), MK-9(H2), MK-9(H6) and MK-9(H10) as menaquinones; diphosphatidylglycerol, phospatidylethanolamine, phosphatidylinositol, phosphotidylinositol mannoside and phosphatidylglycerol as prominent phospholipids; iso-C16 : 0, anteiso-C15 : 0 and C16 : 0 as major cellular fatty acids; and DNA G+C content of 69.9 mol%.
30168793	4	29	theme	genus	364:368	arg1	H6					544:545	H6	544:545	H6	544:545	The strain showed morphological and chemotaxonomic features typical of the genus Streptomyces: branched substrate and aerial mycelia, straight spore chains and smooth spores; ll-diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H8), MK-9(H2), MK-9(H6) and MK-9(H10) as menaquinones; diphosphatidylglycerol, phospatidylethanolamine, phosphatidylinositol, phosphotidylinositol mannoside and phosphatidylglycerol as prominent phospholipids; iso-C16 : 0, anteiso-C15 : 0 and C16 : 0 as major cellular fatty acids; and DNA G+C content of 69.9 mol%.
30168793	4	29	theme	genus	364:368	arg1	phosphatidylglycerol					685:704	phosphatidylglycerol	685:704	phosphatidylglycerol	685:704	The strain showed morphological and chemotaxonomic features typical of the genus Streptomyces: branched substrate and aerial mycelia, straight spore chains and smooth spores; ll-diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H8), MK-9(H2), MK-9(H6) and MK-9(H10) as menaquinones; diphosphatidylglycerol, phospatidylethanolamine, phosphatidylinositol, phosphotidylinositol mannoside and phosphatidylglycerol as prominent phospholipids; iso-C16 : 0, anteiso-C15 : 0 and C16 : 0 as major cellular fatty acids; and DNA G+C content of 69.9 mol%.
30168793	4	29	theme	genus	364:368	arg1	H2					534:535	H2	534:535	H2	534:535	The strain showed morphological and chemotaxonomic features typical of the genus Streptomyces: branched substrate and aerial mycelia, straight spore chains and smooth spores; ll-diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H8), MK-9(H2), MK-9(H6) and MK-9(H10) as menaquinones; diphosphatidylglycerol, phospatidylethanolamine, phosphatidylinositol, phosphotidylinositol mannoside and phosphatidylglycerol as prominent phospholipids; iso-C16 : 0, anteiso-C15 : 0 and C16 : 0 as major cellular fatty acids; and DNA G+C content of 69.9 mol%.
30168793	4	29	theme	genus	364:368	arg1	phosphatidylinositol					628:647	phosphatidylinositol	628:647	phosphatidylinositol	628:647	The strain showed morphological and chemotaxonomic features typical of the genus Streptomyces: branched substrate and aerial mycelia, straight spore chains and smooth spores; ll-diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H8), MK-9(H2), MK-9(H6) and MK-9(H10) as menaquinones; diphosphatidylglycerol, phospatidylethanolamine, phosphatidylinositol, phosphotidylinositol mannoside and phosphatidylglycerol as prominent phospholipids; iso-C16 : 0, anteiso-C15 : 0 and C16 : 0 as major cellular fatty acids; and DNA G+C content of 69.9 mol%.
30168793	5	30	theme	gene	849:852	arg1	analysis					863:870	16S rRNA gene sequence analysis	840:870	16S rRNA gene sequence analysis	840:870	16S rRNA gene sequence analysis indicated that strain XZHG99T showed high similarity to Streptomyces albiflavescensm20T (98.42 %) and Streptomyces krungchingensis KC-035T (98.14 %) as well as formed a monophyletic clade with them in the phylogenetic tree.
30168793	6	31	theme	strain	1183:1188	arg1	XZHG99T					1190:1196	strain XZHG99T	1183:1196	strain XZHG99T	1183:1196	Based on comparison of phenotypic properties and the low level of DNA-DNA relatedness, strain XZHG99T can be distinguished from phylogenetically related Streptomyces species and is suggested to represent a novel species of the genus Streptomyces, for which the name Streptomyces dengpaensis sp.
30168793	1	32	attach	isolated	51:58	arg2	actinomycete					38:49	an actinomycete	35:49	an actinomycete isolated from desert soil	35:75	nov., an actinomycete isolated from desert soil.
30168793	1	32	attach	isolated	51:58	arg1	soil					72:75	desert soil	65:75	desert soil	65:75	nov., an actinomycete isolated from desert soil.
30168793	4	33	theme	%	837:837	arg1	content					818:824	DNA G+C content	810:824	DNA G+C content of 69.9 mol%	810:837	The strain showed morphological and chemotaxonomic features typical of the genus Streptomyces: branched substrate and aerial mycelia, straight spore chains and smooth spores; ll-diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H8), MK-9(H2), MK-9(H6) and MK-9(H10) as menaquinones; diphosphatidylglycerol, phospatidylethanolamine, phosphatidylinositol, phosphotidylinositol mannoside and phosphatidylglycerol as prominent phospholipids; iso-C16 : 0, anteiso-C15 : 0 and C16 : 0 as major cellular fatty acids; and DNA G+C content of 69.9 mol%.
30168793	4	33	theme	%	837:837	arg1	 0					772:773	 0	772:773	 0 as major cellular fatty acids	772:803	The strain showed morphological and chemotaxonomic features typical of the genus Streptomyces: branched substrate and aerial mycelia, straight spore chains and smooth spores; ll-diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H8), MK-9(H2), MK-9(H6) and MK-9(H10) as menaquinones; diphosphatidylglycerol, phospatidylethanolamine, phosphatidylinositol, phosphotidylinositol mannoside and phosphatidylglycerol as prominent phospholipids; iso-C16 : 0, anteiso-C15 : 0 and C16 : 0 as major cellular fatty acids; and DNA G+C content of 69.9 mol%.
30168793	4	33	theme	%	837:837	arg1	C16 					767:770	C16 	767:770	C16 	767:770	The strain showed morphological and chemotaxonomic features typical of the genus Streptomyces: branched substrate and aerial mycelia, straight spore chains and smooth spores; ll-diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H8), MK-9(H2), MK-9(H6) and MK-9(H10) as menaquinones; diphosphatidylglycerol, phospatidylethanolamine, phosphatidylinositol, phosphotidylinositol mannoside and phosphatidylglycerol as prominent phospholipids; iso-C16 : 0, anteiso-C15 : 0 and C16 : 0 as major cellular fatty acids; and DNA G+C content of 69.9 mol%.
30168793	4	33	theme	%	837:837	arg1	 0					760:761	 0	760:761	 0	760:761	The strain showed morphological and chemotaxonomic features typical of the genus Streptomyces: branched substrate and aerial mycelia, straight spore chains and smooth spores; ll-diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H8), MK-9(H2), MK-9(H6) and MK-9(H10) as menaquinones; diphosphatidylglycerol, phospatidylethanolamine, phosphatidylinositol, phosphotidylinositol mannoside and phosphatidylglycerol as prominent phospholipids; iso-C16 : 0, anteiso-C15 : 0 and C16 : 0 as major cellular fatty acids; and DNA G+C content of 69.9 mol%.
30168793	4	33	theme	%	837:837	arg1	features					340:347	morphological and chemotaxonomic features	307:347	morphological and chemotaxonomic features typical of the genus Streptomyces: branched substrate and aerial mycelia, straight spore chains and smooth spores; ll-diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H8), MK-9(H2), MK-9(H6) and MK-9(H10) as menaquinones; diphosphatidylglycerol, phospatidylethanolamine, phosphatidylinositol, phosphotidylinositol mannoside and phosphatidylglycerol as prominent phospholipids; iso-C16 : 0, anteiso-C15 	307:758	The strain showed morphological and chemotaxonomic features typical of the genus Streptomyces: branched substrate and aerial mycelia, straight spore chains and smooth spores; ll-diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H8), MK-9(H2), MK-9(H6) and MK-9(H10) as menaquinones; diphosphatidylglycerol, phospatidylethanolamine, phosphatidylinositol, phosphotidylinositol mannoside and phosphatidylglycerol as prominent phospholipids; iso-C16 : 0, anteiso-C15 : 0 and C16 : 0 as major cellular fatty acids; and DNA G+C content of 69.9 mol%.
30168793	4	34	theme	prominent	709:717	arg1	phospholipids					719:731	prominent phospholipids	709:731	prominent phospholipids	709:731	The strain showed morphological and chemotaxonomic features typical of the genus Streptomyces: branched substrate and aerial mycelia, straight spore chains and smooth spores; ll-diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H8), MK-9(H2), MK-9(H6) and MK-9(H10) as menaquinones; diphosphatidylglycerol, phospatidylethanolamine, phosphatidylinositol, phosphotidylinositol mannoside and phosphatidylglycerol as prominent phospholipids; iso-C16 : 0, anteiso-C15 : 0 and C16 : 0 as major cellular fatty acids; and DNA G+C content of 69.9 mol%.
30168793	4	35	theme	MK-9	552:555	arg1	typical					349:355	typical	349:355	typical	349:355	The strain showed morphological and chemotaxonomic features typical of the genus Streptomyces: branched substrate and aerial mycelia, straight spore chains and smooth spores; ll-diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H8), MK-9(H2), MK-9(H6) and MK-9(H10) as menaquinones; diphosphatidylglycerol, phospatidylethanolamine, phosphatidylinositol, phosphotidylinositol mannoside and phosphatidylglycerol as prominent phospholipids; iso-C16 : 0, anteiso-C15 : 0 and C16 : 0 as major cellular fatty acids; and DNA G+C content of 69.9 mol%.
30168793	4	36	theme	69.9 mol	829:836	arg1	%					837:837	69.9 mol%	829:837	69.9 mol%	829:837	The strain showed morphological and chemotaxonomic features typical of the genus Streptomyces: branched substrate and aerial mycelia, straight spore chains and smooth spores; ll-diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H8), MK-9(H2), MK-9(H6) and MK-9(H10) as menaquinones; diphosphatidylglycerol, phospatidylethanolamine, phosphatidylinositol, phosphotidylinositol mannoside and phosphatidylglycerol as prominent phospholipids; iso-C16 : 0, anteiso-C15 : 0 and C16 : 0 as major cellular fatty acids; and DNA G+C content of 69.9 mol%.
30168793	2	37	theme	colour	161:166	arg1	China					218:222	China	218:222	China	218:222	A novel actinomycete, designated strain XZHG99T, was isolated from soil taken from colour desert, Dengpa District, Tibet Autonomous Region, China.
30168793	2	37	theme	colour	161:166	arg1	Region					210:215	Tibet Autonomous Region	193:215	Tibet Autonomous Region	193:215	A novel actinomycete, designated strain XZHG99T, was isolated from soil taken from colour desert, Dengpa District, Tibet Autonomous Region, China.
30168793	2	37	theme	colour	161:166	arg1	desert					168:173	colour desert	161:173	colour desert	161:173	A novel actinomycete, designated strain XZHG99T, was isolated from soil taken from colour desert, Dengpa District, Tibet Autonomous Region, China.
30168793	2	37	theme	colour	161:166	arg1	District					183:190	Dengpa District	176:190	Dengpa District	176:190	A novel actinomycete, designated strain XZHG99T, was isolated from soil taken from colour desert, Dengpa District, Tibet Autonomous Region, China.
30168793	1	38	dep	actinomycete	38:49	arg1	nov.					29:32	nov.	29:32	nov.	29:32	nov., an actinomycete isolated from desert soil.
30168793	6	39	theme	Streptomyces	1329:1340	arg1	species					1308:1314	a novel species	1300:1314	a novel species	1300:1314	Based on comparison of phenotypic properties and the low level of DNA-DNA relatedness, strain XZHG99T can be distinguished from phylogenetically related Streptomyces species and is suggested to represent a novel species of the genus Streptomyces, for which the name Streptomyces dengpaensis sp.
30168793	3	40	theme	taxonomic	229:237	arg1	position					239:246	Its taxonomic position	225:246	Its taxonomic position	225:246	Its taxonomic position was determined by a polyphasic approach.
30168793	4	41	theme	straight	423:430	arg1	chains					438:443	straight spore chains	423:443	straight spore chains	423:443	The strain showed morphological and chemotaxonomic features typical of the genus Streptomyces: branched substrate and aerial mycelia, straight spore chains and smooth spores; ll-diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H8), MK-9(H2), MK-9(H6) and MK-9(H10) as menaquinones; diphosphatidylglycerol, phospatidylethanolamine, phosphatidylinositol, phosphotidylinositol mannoside and phosphatidylglycerol as prominent phospholipids; iso-C16 : 0, anteiso-C15 : 0 and C16 : 0 as major cellular fatty acids; and DNA G+C content of 69.9 mol%.
30168793	5	42	theme	Streptomyces	928:939	arg1	albiflavescensm20T					941:958	Streptomyces albiflavescensm20T	928:958	Streptomyces albiflavescensm20T (98.42 %)	928:968	16S rRNA gene sequence analysis indicated that strain XZHG99T showed high similarity to Streptomyces albiflavescensm20T (98.42 %) and Streptomyces krungchingensis KC-035T (98.14 %) as well as formed a monophyletic clade with them in the phylogenetic tree.
30168793	5	42	theme	Streptomyces	928:939	arg1	%					967:967	98.42 %	961:967	98.42 %	961:967	16S rRNA gene sequence analysis indicated that strain XZHG99T showed high similarity to Streptomyces albiflavescensm20T (98.42 %) and Streptomyces krungchingensis KC-035T (98.14 %) as well as formed a monophyletic clade with them in the phylogenetic tree.
30168793	6	43	theme	name	1357:1360	arg1	sp					1387:1388	the name Streptomyces dengpaensis sp	1353:1388	the name Streptomyces dengpaensis sp	1353:1388	Based on comparison of phenotypic properties and the low level of DNA-DNA relatedness, strain XZHG99T can be distinguished from phylogenetically related Streptomyces species and is suggested to represent a novel species of the genus Streptomyces, for which the name Streptomyces dengpaensis sp.
30168793	5	44	dep	Streptomyces	974:985	arg1	krungchingensis					987:1001	krungchingensis	987:1001	krungchingensis	987:1001	16S rRNA gene sequence analysis indicated that strain XZHG99T showed high similarity to Streptomyces albiflavescensm20T (98.42 %) and Streptomyces krungchingensis KC-035T (98.14 %) as well as formed a monophyletic clade with them in the phylogenetic tree.
30168793	6	45	theme	related	1241:1247	arg1	species					1262:1268	phylogenetically related Streptomyces species	1224:1268	phylogenetically related Streptomyces species	1224:1268	Based on comparison of phenotypic properties and the low level of DNA-DNA relatedness, strain XZHG99T can be distinguished from phylogenetically related Streptomyces species and is suggested to represent a novel species of the genus Streptomyces, for which the name Streptomyces dengpaensis sp.
30168793	2	46	theme	Autonomous	199:208	arg1	Region					210:215	Tibet Autonomous Region	193:215	Tibet Autonomous Region	193:215	A novel actinomycete, designated strain XZHG99T, was isolated from soil taken from colour desert, Dengpa District, Tibet Autonomous Region, China.
30168793	2	46	theme	Autonomous	199:208	arg1	desert					168:173	colour desert	161:173	colour desert	161:173	A novel actinomycete, designated strain XZHG99T, was isolated from soil taken from colour desert, Dengpa District, Tibet Autonomous Region, China.
30168793	2	47	theme	novel	80:84	arg1	actinomycete					86:97	A novel actinomycete	78:97	A novel actinomycete	78:97	A novel actinomycete, designated strain XZHG99T, was isolated from soil taken from colour desert, Dengpa District, Tibet Autonomous Region, China.
30168793	3	48	theme	polyphasic	268:277	arg1	approach					279:286	a polyphasic approach	266:286	a polyphasic approach	266:286	Its taxonomic position was determined by a polyphasic approach.
30168793	5	49	theme	phylogenetic	1077:1088	arg1	tree					1090:1093	the phylogenetic tree	1073:1093	the phylogenetic tree	1073:1093	16S rRNA gene sequence analysis indicated that strain XZHG99T showed high similarity to Streptomyces albiflavescensm20T (98.42 %) and Streptomyces krungchingensis KC-035T (98.14 %) as well as formed a monophyletic clade with them in the phylogenetic tree.
30168793	2	50	theme	Tibet	193:197	arg1	Region					210:215	Tibet Autonomous Region	193:215	Tibet Autonomous Region	193:215	A novel actinomycete, designated strain XZHG99T, was isolated from soil taken from colour desert, Dengpa District, Tibet Autonomous Region, China.
30168793	2	50	theme	Tibet	193:197	arg1	desert					168:173	colour desert	161:173	colour desert	161:173	A novel actinomycete, designated strain XZHG99T, was isolated from soil taken from colour desert, Dengpa District, Tibet Autonomous Region, China.
30168793	4	51	theme	morphological	307:319	arg1	features					340:347	morphological and chemotaxonomic features	307:347	morphological and chemotaxonomic features typical of the genus Streptomyces: branched substrate and aerial mycelia, straight spore chains and smooth spores; ll-diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H8), MK-9(H2), MK-9(H6) and MK-9(H10) as menaquinones; diphosphatidylglycerol, phospatidylethanolamine, phosphatidylinositol, phosphotidylinositol mannoside and phosphatidylglycerol as prominent phospholipids; iso-C16 : 0, anteiso-C15 	307:758	The strain showed morphological and chemotaxonomic features typical of the genus Streptomyces: branched substrate and aerial mycelia, straight spore chains and smooth spores; ll-diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H8), MK-9(H2), MK-9(H6) and MK-9(H10) as menaquinones; diphosphatidylglycerol, phospatidylethanolamine, phosphatidylinositol, phosphotidylinositol mannoside and phosphatidylglycerol as prominent phospholipids; iso-C16 : 0, anteiso-C15 : 0 and C16 : 0 as major cellular fatty acids; and DNA G+C content of 69.9 mol%.
30168793	2	52	attach	isolated	131:138	arg2	actinomycete					86:97	A novel actinomycete	78:97	A novel actinomycete	78:97	A novel actinomycete, designated strain XZHG99T, was isolated from soil taken from colour desert, Dengpa District, Tibet Autonomous Region, China.
30168793	2	52	attach	isolated	131:138	arg1	soil					145:148	soil	145:148	soil taken from colour desert, Dengpa District, Tibet Autonomous Region, China	145:222	A novel actinomycete, designated strain XZHG99T, was isolated from soil taken from colour desert, Dengpa District, Tibet Autonomous Region, China.
30168793	8	53	theme	=CGMCC	1437:1442	arg1	XZHG99T					1428:1434	XZHG99T	1428:1434	XZHG99T (=CGMCC 4.7472T=KCTC 49090T)	1428:1463	The type strain is XZHG99T (=CGMCC 4.7472T=KCTC 49090T).
30168793	8	53	theme	=CGMCC	1437:1442	arg1	49090T					1457:1462	=CGMCC 4.7472T=KCTC 49090T	1437:1462	=CGMCC 4.7472T=KCTC 49090T	1437:1462	The type strain is XZHG99T (=CGMCC 4.7472T=KCTC 49090T).
30168793	6	54	theme	relatedness	1170:1180	arg1	properties					1130:1139	phenotypic properties	1119:1139	phenotypic properties	1119:1139	Based on comparison of phenotypic properties and the low level of DNA-DNA relatedness, strain XZHG99T can be distinguished from phylogenetically related Streptomyces species and is suggested to represent a novel species of the genus Streptomyces, for which the name Streptomyces dengpaensis sp.
30168793	6	54	theme	relatedness	1170:1180	arg1	level					1153:1157	the low level	1145:1157	the low level of DNA-DNA relatedness	1145:1180	Based on comparison of phenotypic properties and the low level of DNA-DNA relatedness, strain XZHG99T can be distinguished from phylogenetically related Streptomyces species and is suggested to represent a novel species of the genus Streptomyces, for which the name Streptomyces dengpaensis sp.
30168793	4	55	theme	DNA	810:812	arg1	content					818:824	DNA G+C content	810:824	DNA G+C content of 69.9 mol%	810:837	The strain showed morphological and chemotaxonomic features typical of the genus Streptomyces: branched substrate and aerial mycelia, straight spore chains and smooth spores; ll-diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H8), MK-9(H2), MK-9(H6) and MK-9(H10) as menaquinones; diphosphatidylglycerol, phospatidylethanolamine, phosphatidylinositol, phosphotidylinositol mannoside and phosphatidylglycerol as prominent phospholipids; iso-C16 : 0, anteiso-C15 : 0 and C16 : 0 as major cellular fatty acids; and DNA G+C content of 69.9 mol%.
30168793	4	56	theme	aerial	407:412	arg1	mycelia					414:420	aerial mycelia	407:420	aerial mycelia	407:420	The strain showed morphological and chemotaxonomic features typical of the genus Streptomyces: branched substrate and aerial mycelia, straight spore chains and smooth spores; ll-diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H8), MK-9(H2), MK-9(H6) and MK-9(H10) as menaquinones; diphosphatidylglycerol, phospatidylethanolamine, phosphatidylinositol, phosphotidylinositol mannoside and phosphatidylglycerol as prominent phospholipids; iso-C16 : 0, anteiso-C15 : 0 and C16 : 0 as major cellular fatty acids; and DNA G+C content of 69.9 mol%.
30168793	6	57	theme	phenotypic	1119:1128	arg1	properties					1130:1139	phenotypic properties	1119:1139	phenotypic properties	1119:1139	Based on comparison of phenotypic properties and the low level of DNA-DNA relatedness, strain XZHG99T can be distinguished from phylogenetically related Streptomyces species and is suggested to represent a novel species of the genus Streptomyces, for which the name Streptomyces dengpaensis sp.
30168793	6	58	theme	genus	1323:1327	arg1	Streptomyces					1329:1340	the genus Streptomyces	1319:1340	the genus Streptomyces	1319:1340	Based on comparison of phenotypic properties and the low level of DNA-DNA relatedness, strain XZHG99T can be distinguished from phylogenetically related Streptomyces species and is suggested to represent a novel species of the genus Streptomyces, for which the name Streptomyces dengpaensis sp.
30168793	2	59	theme	Dengpa	176:181	arg1	desert					168:173	colour desert	161:173	colour desert	161:173	A novel actinomycete, designated strain XZHG99T, was isolated from soil taken from colour desert, Dengpa District, Tibet Autonomous Region, China.
30168793	2	59	theme	Dengpa	176:181	arg1	District					183:190	Dengpa District	176:190	Dengpa District	176:190	A novel actinomycete, designated strain XZHG99T, was isolated from soil taken from colour desert, Dengpa District, Tibet Autonomous Region, China.
30168793	6	60	theme	level	1153:1157	arg1	comparison					1105:1114	comparison	1105:1114	comparison of phenotypic properties and the low level of DNA-DNA relatedness	1105:1180	Based on comparison of phenotypic properties and the low level of DNA-DNA relatedness, strain XZHG99T can be distinguished from phylogenetically related Streptomyces species and is suggested to represent a novel species of the genus Streptomyces, for which the name Streptomyces dengpaensis sp.
30168793	4	61	theme	chemotaxonomic	325:338	arg1	features					340:347	morphological and chemotaxonomic features	307:347	morphological and chemotaxonomic features typical of the genus Streptomyces: branched substrate and aerial mycelia, straight spore chains and smooth spores; ll-diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H8), MK-9(H2), MK-9(H6) and MK-9(H10) as menaquinones; diphosphatidylglycerol, phospatidylethanolamine, phosphatidylinositol, phosphotidylinositol mannoside and phosphatidylglycerol as prominent phospholipids; iso-C16 : 0, anteiso-C15 	307:758	The strain showed morphological and chemotaxonomic features typical of the genus Streptomyces: branched substrate and aerial mycelia, straight spore chains and smooth spores; ll-diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H8), MK-9(H2), MK-9(H6) and MK-9(H10) as menaquinones; diphosphatidylglycerol, phospatidylethanolamine, phosphatidylinositol, phosphotidylinositol mannoside and phosphatidylglycerol as prominent phospholipids; iso-C16 : 0, anteiso-C15 : 0 and C16 : 0 as major cellular fatty acids; and DNA G+C content of 69.9 mol%.
30168793	5	62	theme	sequence	854:861	arg1	analysis					863:870	16S rRNA gene sequence analysis	840:870	16S rRNA gene sequence analysis	840:870	16S rRNA gene sequence analysis indicated that strain XZHG99T showed high similarity to Streptomyces albiflavescensm20T (98.42 %) and Streptomyces krungchingensis KC-035T (98.14 %) as well as formed a monophyletic clade with them in the phylogenetic tree.
30168793	6	63	theme	properties	1130:1139	arg1	comparison					1105:1114	comparison	1105:1114	comparison of phenotypic properties and the low level of DNA-DNA relatedness	1105:1180	Based on comparison of phenotypic properties and the low level of DNA-DNA relatedness, strain XZHG99T can be distinguished from phylogenetically related Streptomyces species and is suggested to represent a novel species of the genus Streptomyces, for which the name Streptomyces dengpaensis sp.
30168793	5	64	theme	high	909:912	arg1	similarity					914:923	high similarity	909:923	high similarity to Streptomyces albiflavescensm20T (98.42 %) and Streptomyces krungchingensis KC-035T (98.14 %)	909:1019	16S rRNA gene sequence analysis indicated that strain XZHG99T showed high similarity to Streptomyces albiflavescensm20T (98.42 %) and Streptomyces krungchingensis KC-035T (98.14 %) as well as formed a monophyletic clade with them in the phylogenetic tree.
30168793	4	65	theme	phosphotidylinositol	650:669	arg1	Streptomyces					370:381	the genus Streptomyces	360:381	the genus Streptomyces	360:381	The strain showed morphological and chemotaxonomic features typical of the genus Streptomyces: branched substrate and aerial mycelia, straight spore chains and smooth spores; ll-diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H8), MK-9(H2), MK-9(H6) and MK-9(H10) as menaquinones; diphosphatidylglycerol, phospatidylethanolamine, phosphatidylinositol, phosphotidylinositol mannoside and phosphatidylglycerol as prominent phospholipids; iso-C16 : 0, anteiso-C15 : 0 and C16 : 0 as major cellular fatty acids; and DNA G+C content of 69.9 mol%.
30168793	4	65	theme	phosphotidylinositol	650:669	arg1	mannoside					671:679	phosphotidylinositol mannoside	650:679	phosphotidylinositol mannoside	650:679	The strain showed morphological and chemotaxonomic features typical of the genus Streptomyces: branched substrate and aerial mycelia, straight spore chains and smooth spores; ll-diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H8), MK-9(H2), MK-9(H6) and MK-9(H10) as menaquinones; diphosphatidylglycerol, phospatidylethanolamine, phosphatidylinositol, phosphotidylinositol mannoside and phosphatidylglycerol as prominent phospholipids; iso-C16 : 0, anteiso-C15 : 0 and C16 : 0 as major cellular fatty acids; and DNA G+C content of 69.9 mol%.
30168793	5	66	theme	strain	887:892	arg1	XZHG99T					894:900	strain XZHG99T	887:900	strain XZHG99T	887:900	16S rRNA gene sequence analysis indicated that strain XZHG99T showed high similarity to Streptomyces albiflavescensm20T (98.42 %) and Streptomyces krungchingensis KC-035T (98.14 %) as well as formed a monophyletic clade with them in the phylogenetic tree.
30168793	6	67	theme	novel	1302:1306	arg1	species					1308:1314	a novel species	1300:1314	a novel species	1300:1314	Based on comparison of phenotypic properties and the low level of DNA-DNA relatedness, strain XZHG99T can be distinguished from phylogenetically related Streptomyces species and is suggested to represent a novel species of the genus Streptomyces, for which the name Streptomyces dengpaensis sp.
30168793	4	68	theme	diphosphatidylglycerol	579:600	arg1	Streptomyces					370:381	the genus Streptomyces	360:381	the genus Streptomyces	360:381	The strain showed morphological and chemotaxonomic features typical of the genus Streptomyces: branched substrate and aerial mycelia, straight spore chains and smooth spores; ll-diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H8), MK-9(H2), MK-9(H6) and MK-9(H10) as menaquinones; diphosphatidylglycerol, phospatidylethanolamine, phosphatidylinositol, phosphotidylinositol mannoside and phosphatidylglycerol as prominent phospholipids; iso-C16 : 0, anteiso-C15 : 0 and C16 : 0 as major cellular fatty acids; and DNA G+C content of 69.9 mol%.
30168793	4	68	theme	diphosphatidylglycerol	579:600	arg1	mannoside					671:679	phosphotidylinositol mannoside	650:679	phosphotidylinositol mannoside	650:679	The strain showed morphological and chemotaxonomic features typical of the genus Streptomyces: branched substrate and aerial mycelia, straight spore chains and smooth spores; ll-diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H8), MK-9(H2), MK-9(H6) and MK-9(H10) as menaquinones; diphosphatidylglycerol, phospatidylethanolamine, phosphatidylinositol, phosphotidylinositol mannoside and phosphatidylglycerol as prominent phospholipids; iso-C16 : 0, anteiso-C15 : 0 and C16 : 0 as major cellular fatty acids; and DNA G+C content of 69.9 mol%.
30168793	4	68	theme	diphosphatidylglycerol	579:600	arg1	H10					557:559	H10	557:559	H10	557:559	The strain showed morphological and chemotaxonomic features typical of the genus Streptomyces: branched substrate and aerial mycelia, straight spore chains and smooth spores; ll-diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H8), MK-9(H2), MK-9(H6) and MK-9(H10) as menaquinones; diphosphatidylglycerol, phospatidylethanolamine, phosphatidylinositol, phosphotidylinositol mannoside and phosphatidylglycerol as prominent phospholipids; iso-C16 : 0, anteiso-C15 : 0 and C16 : 0 as major cellular fatty acids; and DNA G+C content of 69.9 mol%.
30168793	4	68	theme	diphosphatidylglycerol	579:600	arg1	MK-9					539:542	MK-9	539:542	MK-9	539:542	The strain showed morphological and chemotaxonomic features typical of the genus Streptomyces: branched substrate and aerial mycelia, straight spore chains and smooth spores; ll-diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H8), MK-9(H2), MK-9(H6) and MK-9(H10) as menaquinones; diphosphatidylglycerol, phospatidylethanolamine, phosphatidylinositol, phosphotidylinositol mannoside and phosphatidylglycerol as prominent phospholipids; iso-C16 : 0, anteiso-C15 : 0 and C16 : 0 as major cellular fatty acids; and DNA G+C content of 69.9 mol%.
30168793	4	68	theme	diphosphatidylglycerol	579:600	arg1	MK-9					529:532	MK-9	529:532	MK-9	529:532	The strain showed morphological and chemotaxonomic features typical of the genus Streptomyces: branched substrate and aerial mycelia, straight spore chains and smooth spores; ll-diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H8), MK-9(H2), MK-9(H6) and MK-9(H10) as menaquinones; diphosphatidylglycerol, phospatidylethanolamine, phosphatidylinositol, phosphotidylinositol mannoside and phosphatidylglycerol as prominent phospholipids; iso-C16 : 0, anteiso-C15 : 0 and C16 : 0 as major cellular fatty acids; and DNA G+C content of 69.9 mol%.
30168793	4	68	theme	diphosphatidylglycerol	579:600	arg1	phospatidylethanolamine					603:625	phospatidylethanolamine	603:625	phospatidylethanolamine	603:625	The strain showed morphological and chemotaxonomic features typical of the genus Streptomyces: branched substrate and aerial mycelia, straight spore chains and smooth spores; ll-diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H8), MK-9(H2), MK-9(H6) and MK-9(H10) as menaquinones; diphosphatidylglycerol, phospatidylethanolamine, phosphatidylinositol, phosphotidylinositol mannoside and phosphatidylglycerol as prominent phospholipids; iso-C16 : 0, anteiso-C15 : 0 and C16 : 0 as major cellular fatty acids; and DNA G+C content of 69.9 mol%.
30168793	4	68	theme	diphosphatidylglycerol	579:600	arg1	H6					544:545	H6	544:545	H6	544:545	The strain showed morphological and chemotaxonomic features typical of the genus Streptomyces: branched substrate and aerial mycelia, straight spore chains and smooth spores; ll-diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H8), MK-9(H2), MK-9(H6) and MK-9(H10) as menaquinones; diphosphatidylglycerol, phospatidylethanolamine, phosphatidylinositol, phosphotidylinositol mannoside and phosphatidylglycerol as prominent phospholipids; iso-C16 : 0, anteiso-C15 : 0 and C16 : 0 as major cellular fatty acids; and DNA G+C content of 69.9 mol%.
30168793	4	68	theme	diphosphatidylglycerol	579:600	arg1	phosphatidylglycerol					685:704	phosphatidylglycerol	685:704	phosphatidylglycerol	685:704	The strain showed morphological and chemotaxonomic features typical of the genus Streptomyces: branched substrate and aerial mycelia, straight spore chains and smooth spores; ll-diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H8), MK-9(H2), MK-9(H6) and MK-9(H10) as menaquinones; diphosphatidylglycerol, phospatidylethanolamine, phosphatidylinositol, phosphotidylinositol mannoside and phosphatidylglycerol as prominent phospholipids; iso-C16 : 0, anteiso-C15 : 0 and C16 : 0 as major cellular fatty acids; and DNA G+C content of 69.9 mol%.
30168793	4	68	theme	diphosphatidylglycerol	579:600	arg1	H2					534:535	H2	534:535	H2	534:535	The strain showed morphological and chemotaxonomic features typical of the genus Streptomyces: branched substrate and aerial mycelia, straight spore chains and smooth spores; ll-diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H8), MK-9(H2), MK-9(H6) and MK-9(H10) as menaquinones; diphosphatidylglycerol, phospatidylethanolamine, phosphatidylinositol, phosphotidylinositol mannoside and phosphatidylglycerol as prominent phospholipids; iso-C16 : 0, anteiso-C15 : 0 and C16 : 0 as major cellular fatty acids; and DNA G+C content of 69.9 mol%.
30168793	4	68	theme	diphosphatidylglycerol	579:600	arg1	phosphatidylinositol					628:647	phosphatidylinositol	628:647	phosphatidylinositol	628:647	The strain showed morphological and chemotaxonomic features typical of the genus Streptomyces: branched substrate and aerial mycelia, straight spore chains and smooth spores; ll-diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H8), MK-9(H2), MK-9(H6) and MK-9(H10) as menaquinones; diphosphatidylglycerol, phospatidylethanolamine, phosphatidylinositol, phosphotidylinositol mannoside and phosphatidylglycerol as prominent phospholipids; iso-C16 : 0, anteiso-C15 : 0 and C16 : 0 as major cellular fatty acids; and DNA G+C content of 69.9 mol%.
30307389	8	0	theme	strain	1405:1410	arg1	species					1488:1494	a novel species	1480:1494	a novel species	1480:1494	Overall, strain CM134L-2T belongs to the genus Pedobacter, but can be classified as a novel species, for which the name Pedobacter chitinilyticus sp.
30307389	8	0	theme	strain	1405:1410	arg1	CM134L-2T					1412:1420	strain CM134L-2T	1405:1420	strain CM134L-2T	1405:1420	Overall, strain CM134L-2T belongs to the genus Pedobacter, but can be classified as a novel species, for which the name Pedobacter chitinilyticus sp.
30307389	7	1	dep	%	1090:1090	arg1	 0					1260:1261	 0	1260:1261	 0	1260:1261	The genomic DNA G+C content of strain CM134L-2T was 39.3 mol%, menaquinone-7 was the major respiratory quinone, phosphatidylethanolamine was the major polar lipid and the major components of the cellular fatty acids were iso-C15 : 0, and C16 : 1ω7c/C16 : 1ω6c (summed feature 3); these features supported the affiliation of strain CM134L-2T to the genus Pedobacter.
30307389	7	1	dep	%	1090:1090	arg1	C16 					1268:1271	C16 	1268:1271	C16 	1268:1271	The genomic DNA G+C content of strain CM134L-2T was 39.3 mol%, menaquinone-7 was the major respiratory quinone, phosphatidylethanolamine was the major polar lipid and the major components of the cellular fatty acids were iso-C15 : 0, and C16 : 1ω7c/C16 : 1ω6c (summed feature 3); these features supported the affiliation of strain CM134L-2T to the genus Pedobacter.
30307389	7	2	theme	major	1115:1119	arg1	quinone					1133:1139	the major respiratory quinone	1111:1139	the major respiratory quinone	1111:1139	The genomic DNA G+C content of strain CM134L-2T was 39.3 mol%, menaquinone-7 was the major respiratory quinone, phosphatidylethanolamine was the major polar lipid and the major components of the cellular fatty acids were iso-C15 : 0, and C16 : 1ω7c/C16 : 1ω6c (summed feature 3); these features supported the affiliation of strain CM134L-2T to the genus Pedobacter.
30307389	7	2	theme	major	1115:1119	arg1	menaquinone-7					1093:1105	menaquinone-7	1093:1105	menaquinone-7	1093:1105	The genomic DNA G+C content of strain CM134L-2T was 39.3 mol%, menaquinone-7 was the major respiratory quinone, phosphatidylethanolamine was the major polar lipid and the major components of the cellular fatty acids were iso-C15 : 0, and C16 : 1ω7c/C16 : 1ω6c (summed feature 3); these features supported the affiliation of strain CM134L-2T to the genus Pedobacter.
30307389	6	3	dep	boundaries	921:930	arg1	suggests					976:983	suggests	976:983	suggests strain CM134L-2T represents a novel species	976:1027	Digital DNA-DNA hybridization (dDDH) values between strain CM134L-2T with these two type strains were 26.8 and 20.8 %, respectively, and average nucleotide identity (ANI) values were 83.2 and 76.2 %; these values are lower than the proposed and generally accepted species boundaries of 70 % for dDDH and 95-96 % for ANI, which suggests strain CM134L-2T represents a novel species.
30307389	6	4	theme	accepted	904:911	arg1	species					913:919	the proposed and generally accepted species	877:919	the proposed and generally accepted species	877:919	Digital DNA-DNA hybridization (dDDH) values between strain CM134L-2T with these two type strains were 26.8 and 20.8 %, respectively, and average nucleotide identity (ANI) values were 83.2 and 76.2 %; these values are lower than the proposed and generally accepted species boundaries of 70 % for dDDH and 95-96 % for ANI, which suggests strain CM134L-2T represents a novel species.
30307389	3	5	theme	rod-shaped	308:317	arg1	yellow					331:336	Gram-stain-negative, strictly aerobic, non-spore-forming, motile, rod-shaped, and bright yellow	242:336	Gram-stain-negative, strictly aerobic, non-spore-forming, motile, rod-shaped, and bright yellow	242:336	It was Gram-stain-negative, strictly aerobic, non-spore-forming, motile, rod-shaped, and bright yellow in colour.
30307389	5	6	theme	Pedobacter	601:610	arg1	%					645:645	97.4 %	640:645	97.4 %	640:645	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CM134L-2T was most closely related to Pedobacter nanyangensis Q-4T (97.7 %) and Pedobacter zeaxanthinifaciens TDMA-5T (97.4 %).
30307389	5	6	theme	Pedobacter	601:610	arg1	TDMA-5T					631:637	Pedobacter zeaxanthinifaciens TDMA-5T	601:637	Pedobacter zeaxanthinifaciens TDMA-5T (97.4 %)	601:646	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CM134L-2T was most closely related to Pedobacter nanyangensis Q-4T (97.7 %) and Pedobacter zeaxanthinifaciens TDMA-5T (97.4 %).
30307389	7	7	theme	respiratory	1121:1131	arg1	quinone					1133:1139	the major respiratory quinone	1111:1139	the major respiratory quinone	1111:1139	The genomic DNA G+C content of strain CM134L-2T was 39.3 mol%, menaquinone-7 was the major respiratory quinone, phosphatidylethanolamine was the major polar lipid and the major components of the cellular fatty acids were iso-C15 : 0, and C16 : 1ω7c/C16 : 1ω6c (summed feature 3); these features supported the affiliation of strain CM134L-2T to the genus Pedobacter.
30307389	7	7	theme	respiratory	1121:1131	arg1	menaquinone-7					1093:1105	menaquinone-7	1093:1105	menaquinone-7	1093:1105	The genomic DNA G+C content of strain CM134L-2T was 39.3 mol%, menaquinone-7 was the major respiratory quinone, phosphatidylethanolamine was the major polar lipid and the major components of the cellular fatty acids were iso-C15 : 0, and C16 : 1ω7c/C16 : 1ω6c (summed feature 3); these features supported the affiliation of strain CM134L-2T to the genus Pedobacter.
30307389	8	8	theme	name	1511:1514	arg1	sp					1542:1543	the name Pedobacter chitinilyticus sp	1507:1543	the name Pedobacter chitinilyticus sp	1507:1543	Overall, strain CM134L-2T belongs to the genus Pedobacter, but can be classified as a novel species, for which the name Pedobacter chitinilyticus sp.
30307389	6	9	theme	identity	805:812	arg1	values					820:825	average nucleotide identity (ANI) values	786:825	average nucleotide identity (ANI) values	786:825	Digital DNA-DNA hybridization (dDDH) values between strain CM134L-2T with these two type strains were 26.8 and 20.8 %, respectively, and average nucleotide identity (ANI) values were 83.2 and 76.2 %; these values are lower than the proposed and generally accepted species boundaries of 70 % for dDDH and 95-96 % for ANI, which suggests strain CM134L-2T represents a novel species.
30307389	2	10	from	microbiome	187:196	arg1	Sichuan					210:216	Sichuan	210:216	Sichuan	210:216	A bacterium, designated strain CM134L-2T, was isolated from a chitin-enriched wheat leaf microbiome in Chengdu, Sichuan province, China.
30307389	2	10	from	microbiome	187:196	arg1	China					228:232	China	228:232	China	228:232	A bacterium, designated strain CM134L-2T, was isolated from a chitin-enriched wheat leaf microbiome in Chengdu, Sichuan province, China.
30307389	5	11	theme	zeaxanthinifaciens	612:629	arg1	%					645:645	97.4 %	640:645	97.4 %	640:645	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CM134L-2T was most closely related to Pedobacter nanyangensis Q-4T (97.7 %) and Pedobacter zeaxanthinifaciens TDMA-5T (97.4 %).
30307389	5	11	theme	zeaxanthinifaciens	612:629	arg1	TDMA-5T					631:637	Pedobacter zeaxanthinifaciens TDMA-5T	601:637	Pedobacter zeaxanthinifaciens TDMA-5T (97.4 %)	601:646	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CM134L-2T was most closely related to Pedobacter nanyangensis Q-4T (97.7 %) and Pedobacter zeaxanthinifaciens TDMA-5T (97.4 %).
30307389	5	12	theme	Pedobacter	559:568	arg1	%					594:594	97.7 %	589:594	97.7 %	589:594	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CM134L-2T was most closely related to Pedobacter nanyangensis Q-4T (97.7 %) and Pedobacter zeaxanthinifaciens TDMA-5T (97.4 %).
30307389	5	12	theme	Pedobacter	559:568	arg1	Q-4T					583:586	Pedobacter nanyangensis Q-4T	559:586	Pedobacter nanyangensis Q-4T (97.7 %)	559:595	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CM134L-2T was most closely related to Pedobacter nanyangensis Q-4T (97.7 %) and Pedobacter zeaxanthinifaciens TDMA-5T (97.4 %).
30307389	8	13	theme	chitinilyticus	1527:1540	arg1	sp					1542:1543	the name Pedobacter chitinilyticus sp	1507:1543	the name Pedobacter chitinilyticus sp	1507:1543	Overall, strain CM134L-2T belongs to the genus Pedobacter, but can be classified as a novel species, for which the name Pedobacter chitinilyticus sp.
30307389	10	14	theme	1.16520T=KCTC	1601:1613	arg1	62643T					1615:1620	=CGMCC 1.16520T=KCTC 62643T	1594:1620	=CGMCC 1.16520T=KCTC 62643T	1594:1620	The type strain is CM134L-2T (=CGMCC 1.16520T=KCTC 62643T).
30307389	10	14	theme	1.16520T=KCTC	1601:1613	arg1	CM134L-2T					1583:1591	CM134L-2T	1583:1591	CM134L-2T (=CGMCC 1.16520T=KCTC 62643T)	1583:1621	The type strain is CM134L-2T (=CGMCC 1.16520T=KCTC 62643T).
30307389	4	15	theme	carbon	430:435	arg1	resource					437:444	the only carbon resource	421:444	the only carbon resource	421:444	Strain CM134L-2T grew at 4-35 °C, at pH 6.0-9.0 and could use chitin as the only carbon resource.
30307389	4	15	theme	carbon	430:435	arg1	chitin					411:416	chitin	411:416	chitin	411:416	Strain CM134L-2T grew at 4-35 °C, at pH 6.0-9.0 and could use chitin as the only carbon resource.
30307389	5	16	theme	nanyangensis	570:581	arg1	%					594:594	97.7 %	589:594	97.7 %	589:594	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CM134L-2T was most closely related to Pedobacter nanyangensis Q-4T (97.7 %) and Pedobacter zeaxanthinifaciens TDMA-5T (97.4 %).
30307389	5	16	theme	nanyangensis	570:581	arg1	Q-4T					583:586	Pedobacter nanyangensis Q-4T	559:586	Pedobacter nanyangensis Q-4T (97.7 %)	559:595	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CM134L-2T was most closely related to Pedobacter nanyangensis Q-4T (97.7 %) and Pedobacter zeaxanthinifaciens TDMA-5T (97.4 %).
30307389	7	17	theme	summed	1291:1296	arg1	 1ω6c					1284:1288	 1ω6c	1284:1288	 0, and C16 : 1ω7c/C16 : 1ω6c (summed feature 3); these features supported the affiliation of strain CM134L-2T to the genus Pedobacter	1260:1393	The genomic DNA G+C content of strain CM134L-2T was 39.3 mol%, menaquinone-7 was the major respiratory quinone, phosphatidylethanolamine was the major polar lipid and the major components of the cellular fatty acids were iso-C15 : 0, and C16 : 1ω7c/C16 : 1ω6c (summed feature 3); these features supported the affiliation of strain CM134L-2T to the genus Pedobacter.
30307389	7	17	theme	summed	1291:1296	arg1	feature					1298:1304	summed feature 3	1291:1306	summed feature 3	1291:1306	The genomic DNA G+C content of strain CM134L-2T was 39.3 mol%, menaquinone-7 was the major respiratory quinone, phosphatidylethanolamine was the major polar lipid and the major components of the cellular fatty acids were iso-C15 : 0, and C16 : 1ω7c/C16 : 1ω6c (summed feature 3); these features supported the affiliation of strain CM134L-2T to the genus Pedobacter.
30307389	5	18	theme	Phylogenetic	447:458	arg1	analysis					460:467	Phylogenetic analysis	447:467	Phylogenetic analysis based on 16S rRNA gene sequences	447:500	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CM134L-2T was most closely related to Pedobacter nanyangensis Q-4T (97.7 %) and Pedobacter zeaxanthinifaciens TDMA-5T (97.4 %).
30307389	6	19	theme	proposed	881:888	arg1	species					913:919	the proposed and generally accepted species	877:919	the proposed and generally accepted species	877:919	Digital DNA-DNA hybridization (dDDH) values between strain CM134L-2T with these two type strains were 26.8 and 20.8 %, respectively, and average nucleotide identity (ANI) values were 83.2 and 76.2 %; these values are lower than the proposed and generally accepted species boundaries of 70 % for dDDH and 95-96 % for ANI, which suggests strain CM134L-2T represents a novel species.
30307389	3	20	theme	bright	324:329	arg1	yellow					331:336	Gram-stain-negative, strictly aerobic, non-spore-forming, motile, rod-shaped, and bright yellow	242:336	Gram-stain-negative, strictly aerobic, non-spore-forming, motile, rod-shaped, and bright yellow	242:336	It was Gram-stain-negative, strictly aerobic, non-spore-forming, motile, rod-shaped, and bright yellow in colour.
30307389	4	21	theme	only	425:428	arg1	resource					437:444	the only carbon resource	421:444	the only carbon resource	421:444	Strain CM134L-2T grew at 4-35 °C, at pH 6.0-9.0 and could use chitin as the only carbon resource.
30307389	4	21	theme	only	425:428	arg1	chitin					411:416	chitin	411:416	chitin	411:416	Strain CM134L-2T grew at 4-35 °C, at pH 6.0-9.0 and could use chitin as the only carbon resource.
30307389	7	22	dep	 0	1260:1261	arg1	 1ω6c					1284:1288	 1ω6c	1284:1288	 0, and C16 : 1ω7c/C16 : 1ω6c (summed feature 3); these features supported the affiliation of strain CM134L-2T to the genus Pedobacter	1260:1393	The genomic DNA G+C content of strain CM134L-2T was 39.3 mol%, menaquinone-7 was the major respiratory quinone, phosphatidylethanolamine was the major polar lipid and the major components of the cellular fatty acids were iso-C15 : 0, and C16 : 1ω7c/C16 : 1ω6c (summed feature 3); these features supported the affiliation of strain CM134L-2T to the genus Pedobacter.
30307389	7	22	dep	 0	1260:1261	arg1	feature					1298:1304	summed feature 3	1291:1306	summed feature 3	1291:1306	The genomic DNA G+C content of strain CM134L-2T was 39.3 mol%, menaquinone-7 was the major respiratory quinone, phosphatidylethanolamine was the major polar lipid and the major components of the cellular fatty acids were iso-C15 : 0, and C16 : 1ω7c/C16 : 1ω6c (summed feature 3); these features supported the affiliation of strain CM134L-2T to the genus Pedobacter.
30307389	7	22	dep	 0	1260:1261	arg1	 1ω7c/C16 					1273:1282	 1ω7c/C16 	1273:1282	 0, and C16 : 1ω7c/C16 : 1ω6c (summed feature 3); these features supported the affiliation of strain CM134L-2T to the genus Pedobacter	1260:1393	The genomic DNA G+C content of strain CM134L-2T was 39.3 mol%, menaquinone-7 was the major respiratory quinone, phosphatidylethanolamine was the major polar lipid and the major components of the cellular fatty acids were iso-C15 : 0, and C16 : 1ω7c/C16 : 1ω6c (summed feature 3); these features supported the affiliation of strain CM134L-2T to the genus Pedobacter.
30307389	7	22	dep	 0	1260:1261	arg1	supported					1325:1333	supported	1325:1333	supported the affiliation of strain CM134L-2T to the genus Pedobacter	1325:1393	The genomic DNA G+C content of strain CM134L-2T was 39.3 mol%, menaquinone-7 was the major respiratory quinone, phosphatidylethanolamine was the major polar lipid and the major components of the cellular fatty acids were iso-C15 : 0, and C16 : 1ω7c/C16 : 1ω6c (summed feature 3); these features supported the affiliation of strain CM134L-2T to the genus Pedobacter.
30307389	10	23	theme	type	1568:1571	arg1	strain					1573:1578	The type strain	1564:1578	The type strain	1564:1578	The type strain is CM134L-2T (=CGMCC 1.16520T=KCTC 62643T).
30307389	10	23	theme	type	1568:1571	arg1	CM134L-2T					1583:1591	CM134L-2T	1583:1591	CM134L-2T (=CGMCC 1.16520T=KCTC 62643T)	1583:1621	The type strain is CM134L-2T (=CGMCC 1.16520T=KCTC 62643T).
30307389	6	24	theme	DNA-DNA	657:663	arg1	dDDH					680:683	dDDH	680:683	dDDH	680:683	Digital DNA-DNA hybridization (dDDH) values between strain CM134L-2T with these two type strains were 26.8 and 20.8 %, respectively, and average nucleotide identity (ANI) values were 83.2 and 76.2 %; these values are lower than the proposed and generally accepted species boundaries of 70 % for dDDH and 95-96 % for ANI, which suggests strain CM134L-2T represents a novel species.
30307389	6	24	theme	DNA-DNA	657:663	arg1	hybridization					665:677	Digital DNA-DNA hybridization	649:677	Digital DNA-DNA hybridization (dDDH) values between strain CM134L-2T with these two type strains	649:744	Digital DNA-DNA hybridization (dDDH) values between strain CM134L-2T with these two type strains were 26.8 and 20.8 %, respectively, and average nucleotide identity (ANI) values were 83.2 and 76.2 %; these values are lower than the proposed and generally accepted species boundaries of 70 % for dDDH and 95-96 % for ANI, which suggests strain CM134L-2T represents a novel species.
30307389	6	25	theme	ANI	815:817	arg1	values					820:825	average nucleotide identity (ANI) values	786:825	average nucleotide identity (ANI) values	786:825	Digital DNA-DNA hybridization (dDDH) values between strain CM134L-2T with these two type strains were 26.8 and 20.8 %, respectively, and average nucleotide identity (ANI) values were 83.2 and 76.2 %; these values are lower than the proposed and generally accepted species boundaries of 70 % for dDDH and 95-96 % for ANI, which suggests strain CM134L-2T represents a novel species.
30307389	7	26	theme	strain	1061:1066	arg1	CM134L-2T					1068:1076	strain CM134L-2T	1061:1076	strain CM134L-2T	1061:1076	The genomic DNA G+C content of strain CM134L-2T was 39.3 mol%, menaquinone-7 was the major respiratory quinone, phosphatidylethanolamine was the major polar lipid and the major components of the cellular fatty acids were iso-C15 : 0, and C16 : 1ω7c/C16 : 1ω6c (summed feature 3); these features supported the affiliation of strain CM134L-2T to the genus Pedobacter.
30307389	1	27	theme	wheat	79:83	arg1	tissue					90:95	wheat leaf tissue	79:95	wheat leaf tissue	79:95	nov., a chitin-degrading bacterium isolated from wheat leaf tissue.
30307389	6	28	theme	Digital	649:655	arg1	dDDH					680:683	dDDH	680:683	dDDH	680:683	Digital DNA-DNA hybridization (dDDH) values between strain CM134L-2T with these two type strains were 26.8 and 20.8 %, respectively, and average nucleotide identity (ANI) values were 83.2 and 76.2 %; these values are lower than the proposed and generally accepted species boundaries of 70 % for dDDH and 95-96 % for ANI, which suggests strain CM134L-2T represents a novel species.
30307389	6	28	theme	Digital	649:655	arg1	hybridization					665:677	Digital DNA-DNA hybridization	649:677	Digital DNA-DNA hybridization (dDDH) values between strain CM134L-2T with these two type strains	649:744	Digital DNA-DNA hybridization (dDDH) values between strain CM134L-2T with these two type strains were 26.8 and 20.8 %, respectively, and average nucleotide identity (ANI) values were 83.2 and 76.2 %; these values are lower than the proposed and generally accepted species boundaries of 70 % for dDDH and 95-96 % for ANI, which suggests strain CM134L-2T represents a novel species.
30307389	7	29	theme	CM134L-2T	1068:1076	arg1	G+C content					1046:1056	The genomic DNA G+C content	1030:1056	The genomic DNA G+C content of strain CM134L-2T	1030:1076	The genomic DNA G+C content of strain CM134L-2T was 39.3 mol%, menaquinone-7 was the major respiratory quinone, phosphatidylethanolamine was the major polar lipid and the major components of the cellular fatty acids were iso-C15 : 0, and C16 : 1ω7c/C16 : 1ω6c (summed feature 3); these features supported the affiliation of strain CM134L-2T to the genus Pedobacter.
30307389	7	29	theme	CM134L-2T	1068:1076	arg1	%					1090:1090	39.3 mol%	1082:1090	39.3 mol%	1082:1090	The genomic DNA G+C content of strain CM134L-2T was 39.3 mol%, menaquinone-7 was the major respiratory quinone, phosphatidylethanolamine was the major polar lipid and the major components of the cellular fatty acids were iso-C15 : 0, and C16 : 1ω7c/C16 : 1ω6c (summed feature 3); these features supported the affiliation of strain CM134L-2T to the genus Pedobacter.
30307389	0	30	theme	chitinilyticus	11:24	arg1	sp					26:27	Pedobacter chitinilyticus sp	0:27	Pedobacter chitinilyticus sp.	0:28	Pedobacter chitinilyticus sp.
30307389	6	31	theme	nucleotide	794:803	arg1	values					820:825	average nucleotide identity (ANI) values	786:825	average nucleotide identity (ANI) values	786:825	Digital DNA-DNA hybridization (dDDH) values between strain CM134L-2T with these two type strains were 26.8 and 20.8 %, respectively, and average nucleotide identity (ANI) values were 83.2 and 76.2 %; these values are lower than the proposed and generally accepted species boundaries of 70 % for dDDH and 95-96 % for ANI, which suggests strain CM134L-2T represents a novel species.
30307389	6	32	theme	type	733:736	arg1	strains					738:744	these two type strains	723:744	these two type strains	723:744	Digital DNA-DNA hybridization (dDDH) values between strain CM134L-2T with these two type strains were 26.8 and 20.8 %, respectively, and average nucleotide identity (ANI) values were 83.2 and 76.2 %; these values are lower than the proposed and generally accepted species boundaries of 70 % for dDDH and 95-96 % for ANI, which suggests strain CM134L-2T represents a novel species.
30307389	1	33	theme	leaf	85:88	arg1	tissue					90:95	wheat leaf tissue	79:95	wheat leaf tissue	79:95	nov., a chitin-degrading bacterium isolated from wheat leaf tissue.
30307389	6	34	theme	%	959:959	arg1	boundaries					921:930	lower than the proposed and generally accepted species boundaries	866:930	lower than the proposed and generally accepted species boundaries of 70 % for dDDH and 95-96 % for ANI	866:967	Digital DNA-DNA hybridization (dDDH) values between strain CM134L-2T with these two type strains were 26.8 and 20.8 %, respectively, and average nucleotide identity (ANI) values were 83.2 and 76.2 %; these values are lower than the proposed and generally accepted species boundaries of 70 % for dDDH and 95-96 % for ANI, which suggests strain CM134L-2T represents a novel species.
30307389	6	34	theme	%	959:959	arg1	values					855:860	these values	849:860	these values	849:860	Digital DNA-DNA hybridization (dDDH) values between strain CM134L-2T with these two type strains were 26.8 and 20.8 %, respectively, and average nucleotide identity (ANI) values were 83.2 and 76.2 %; these values are lower than the proposed and generally accepted species boundaries of 70 % for dDDH and 95-96 % for ANI, which suggests strain CM134L-2T represents a novel species.
30307389	0	35	theme	Pedobacter	0:9	arg1	sp					26:27	Pedobacter chitinilyticus sp	0:27	Pedobacter chitinilyticus sp.	0:28	Pedobacter chitinilyticus sp.
30307389	10	36	theme	=CGMCC	1594:1599	arg1	62643T					1615:1620	=CGMCC 1.16520T=KCTC 62643T	1594:1620	=CGMCC 1.16520T=KCTC 62643T	1594:1620	The type strain is CM134L-2T (=CGMCC 1.16520T=KCTC 62643T).
30307389	10	36	theme	=CGMCC	1594:1599	arg1	CM134L-2T					1583:1591	CM134L-2T	1583:1591	CM134L-2T (=CGMCC 1.16520T=KCTC 62643T)	1583:1621	The type strain is CM134L-2T (=CGMCC 1.16520T=KCTC 62643T).
30307389	3	37	from	yellow	331:336	arg1	colour					341:346	colour	341:346	colour	341:346	It was Gram-stain-negative, strictly aerobic, non-spore-forming, motile, rod-shaped, and bright yellow in colour.
30307389	6	38	with	CM134L-2T	708:716	arg1	strains					738:744	these two type strains	723:744	these two type strains	723:744	Digital DNA-DNA hybridization (dDDH) values between strain CM134L-2T with these two type strains were 26.8 and 20.8 %, respectively, and average nucleotide identity (ANI) values were 83.2 and 76.2 %; these values are lower than the proposed and generally accepted species boundaries of 70 % for dDDH and 95-96 % for ANI, which suggests strain CM134L-2T represents a novel species.
30307389	6	39	theme	lower	866:870	arg1	boundaries					921:930	lower than the proposed and generally accepted species boundaries	866:930	lower than the proposed and generally accepted species boundaries of 70 % for dDDH and 95-96 % for ANI	866:967	Digital DNA-DNA hybridization (dDDH) values between strain CM134L-2T with these two type strains were 26.8 and 20.8 %, respectively, and average nucleotide identity (ANI) values were 83.2 and 76.2 %; these values are lower than the proposed and generally accepted species boundaries of 70 % for dDDH and 95-96 % for ANI, which suggests strain CM134L-2T represents a novel species.
30307389	6	39	theme	lower	866:870	arg1	values					855:860	these values	849:860	these values	849:860	Digital DNA-DNA hybridization (dDDH) values between strain CM134L-2T with these two type strains were 26.8 and 20.8 %, respectively, and average nucleotide identity (ANI) values were 83.2 and 76.2 %; these values are lower than the proposed and generally accepted species boundaries of 70 % for dDDH and 95-96 % for ANI, which suggests strain CM134L-2T represents a novel species.
30307389	6	40	theme	strain	701:706	arg1	CM134L-2T					708:716	strain CM134L-2T	701:716	strain CM134L-2T with these two type strains	701:744	Digital DNA-DNA hybridization (dDDH) values between strain CM134L-2T with these two type strains were 26.8 and 20.8 %, respectively, and average nucleotide identity (ANI) values were 83.2 and 76.2 %; these values are lower than the proposed and generally accepted species boundaries of 70 % for dDDH and 95-96 % for ANI, which suggests strain CM134L-2T represents a novel species.
30307389	2	41	theme	wheat	176:180	arg1	microbiome					187:196	a chitin-enriched wheat leaf microbiome	158:196	a chitin-enriched wheat leaf microbiome in Chengdu, Sichuan province, China	158:232	A bacterium, designated strain CM134L-2T, was isolated from a chitin-enriched wheat leaf microbiome in Chengdu, Sichuan province, China.
30307389	7	42	theme	major	1201:1205	arg1	components					1207:1216	the major components	1197:1216	the major components of the cellular fatty acids	1197:1244	The genomic DNA G+C content of strain CM134L-2T was 39.3 mol%, menaquinone-7 was the major respiratory quinone, phosphatidylethanolamine was the major polar lipid and the major components of the cellular fatty acids were iso-C15 : 0, and C16 : 1ω7c/C16 : 1ω6c (summed feature 3); these features supported the affiliation of strain CM134L-2T to the genus Pedobacter.
30307389	7	42	theme	major	1201:1205	arg1	iso-C15 					1251:1258	iso-C15 	1251:1258	iso-C15 	1251:1258	The genomic DNA G+C content of strain CM134L-2T was 39.3 mol%, menaquinone-7 was the major respiratory quinone, phosphatidylethanolamine was the major polar lipid and the major components of the cellular fatty acids were iso-C15 : 0, and C16 : 1ω7c/C16 : 1ω6c (summed feature 3); these features supported the affiliation of strain CM134L-2T to the genus Pedobacter.
30307389	7	43	theme	major	1175:1179	arg1	lipid					1187:1191	the major polar lipid	1171:1191	the major polar lipid	1171:1191	The genomic DNA G+C content of strain CM134L-2T was 39.3 mol%, menaquinone-7 was the major respiratory quinone, phosphatidylethanolamine was the major polar lipid and the major components of the cellular fatty acids were iso-C15 : 0, and C16 : 1ω7c/C16 : 1ω6c (summed feature 3); these features supported the affiliation of strain CM134L-2T to the genus Pedobacter.
30307389	7	43	theme	major	1175:1179	arg1	phosphatidylethanolamine					1142:1165	phosphatidylethanolamine	1142:1165	phosphatidylethanolamine	1142:1165	The genomic DNA G+C content of strain CM134L-2T was 39.3 mol%, menaquinone-7 was the major respiratory quinone, phosphatidylethanolamine was the major polar lipid and the major components of the cellular fatty acids were iso-C15 : 0, and C16 : 1ω7c/C16 : 1ω6c (summed feature 3); these features supported the affiliation of strain CM134L-2T to the genus Pedobacter.
30307389	3	44	theme	Gram-stain-negative	242:260	arg1	yellow					331:336	Gram-stain-negative, strictly aerobic, non-spore-forming, motile, rod-shaped, and bright yellow	242:336	Gram-stain-negative, strictly aerobic, non-spore-forming, motile, rod-shaped, and bright yellow	242:336	It was Gram-stain-negative, strictly aerobic, non-spore-forming, motile, rod-shaped, and bright yellow in colour.
30307389	2	45	theme	chitin-enriched	160:174	arg1	microbiome					187:196	a chitin-enriched wheat leaf microbiome	158:196	a chitin-enriched wheat leaf microbiome in Chengdu, Sichuan province, China	158:232	A bacterium, designated strain CM134L-2T, was isolated from a chitin-enriched wheat leaf microbiome in Chengdu, Sichuan province, China.
30307389	7	46	theme	polar	1181:1185	arg1	lipid					1187:1191	the major polar lipid	1171:1191	the major polar lipid	1171:1191	The genomic DNA G+C content of strain CM134L-2T was 39.3 mol%, menaquinone-7 was the major respiratory quinone, phosphatidylethanolamine was the major polar lipid and the major components of the cellular fatty acids were iso-C15 : 0, and C16 : 1ω7c/C16 : 1ω6c (summed feature 3); these features supported the affiliation of strain CM134L-2T to the genus Pedobacter.
30307389	7	46	theme	polar	1181:1185	arg1	phosphatidylethanolamine					1142:1165	phosphatidylethanolamine	1142:1165	phosphatidylethanolamine	1142:1165	The genomic DNA G+C content of strain CM134L-2T was 39.3 mol%, menaquinone-7 was the major respiratory quinone, phosphatidylethanolamine was the major polar lipid and the major components of the cellular fatty acids were iso-C15 : 0, and C16 : 1ω7c/C16 : 1ω6c (summed feature 3); these features supported the affiliation of strain CM134L-2T to the genus Pedobacter.
30307389	7	47	theme	genomic	1034:1040	arg1	G+C content					1046:1056	The genomic DNA G+C content	1030:1056	The genomic DNA G+C content of strain CM134L-2T	1030:1076	The genomic DNA G+C content of strain CM134L-2T was 39.3 mol%, menaquinone-7 was the major respiratory quinone, phosphatidylethanolamine was the major polar lipid and the major components of the cellular fatty acids were iso-C15 : 0, and C16 : 1ω7c/C16 : 1ω6c (summed feature 3); these features supported the affiliation of strain CM134L-2T to the genus Pedobacter.
30307389	7	47	theme	genomic	1034:1040	arg1	%					1090:1090	39.3 mol%	1082:1090	39.3 mol%	1082:1090	The genomic DNA G+C content of strain CM134L-2T was 39.3 mol%, menaquinone-7 was the major respiratory quinone, phosphatidylethanolamine was the major polar lipid and the major components of the cellular fatty acids were iso-C15 : 0, and C16 : 1ω7c/C16 : 1ω6c (summed feature 3); these features supported the affiliation of strain CM134L-2T to the genus Pedobacter.
30307389	6	48	theme	average	786:792	arg1	values					820:825	average nucleotide identity (ANI) values	786:825	average nucleotide identity (ANI) values	786:825	Digital DNA-DNA hybridization (dDDH) values between strain CM134L-2T with these two type strains were 26.8 and 20.8 %, respectively, and average nucleotide identity (ANI) values were 83.2 and 76.2 %; these values are lower than the proposed and generally accepted species boundaries of 70 % for dDDH and 95-96 % for ANI, which suggests strain CM134L-2T represents a novel species.
30307389	7	49	theme	DNA	1042:1044	arg1	G+C content					1046:1056	The genomic DNA G+C content	1030:1056	The genomic DNA G+C content of strain CM134L-2T	1030:1076	The genomic DNA G+C content of strain CM134L-2T was 39.3 mol%, menaquinone-7 was the major respiratory quinone, phosphatidylethanolamine was the major polar lipid and the major components of the cellular fatty acids were iso-C15 : 0, and C16 : 1ω7c/C16 : 1ω6c (summed feature 3); these features supported the affiliation of strain CM134L-2T to the genus Pedobacter.
30307389	7	49	theme	DNA	1042:1044	arg1	%					1090:1090	39.3 mol%	1082:1090	39.3 mol%	1082:1090	The genomic DNA G+C content of strain CM134L-2T was 39.3 mol%, menaquinone-7 was the major respiratory quinone, phosphatidylethanolamine was the major polar lipid and the major components of the cellular fatty acids were iso-C15 : 0, and C16 : 1ω7c/C16 : 1ω6c (summed feature 3); these features supported the affiliation of strain CM134L-2T to the genus Pedobacter.
30307389	6	50	theme	%	938:938	arg1	boundaries					921:930	lower than the proposed and generally accepted species boundaries	866:930	lower than the proposed and generally accepted species boundaries of 70 % for dDDH and 95-96 % for ANI	866:967	Digital DNA-DNA hybridization (dDDH) values between strain CM134L-2T with these two type strains were 26.8 and 20.8 %, respectively, and average nucleotide identity (ANI) values were 83.2 and 76.2 %; these values are lower than the proposed and generally accepted species boundaries of 70 % for dDDH and 95-96 % for ANI, which suggests strain CM134L-2T represents a novel species.
30307389	6	50	theme	%	938:938	arg1	values					855:860	these values	849:860	these values	849:860	Digital DNA-DNA hybridization (dDDH) values between strain CM134L-2T with these two type strains were 26.8 and 20.8 %, respectively, and average nucleotide identity (ANI) values were 83.2 and 76.2 %; these values are lower than the proposed and generally accepted species boundaries of 70 % for dDDH and 95-96 % for ANI, which suggests strain CM134L-2T represents a novel species.
30307389	7	51	theme	39.3 mol	1082:1089	arg1	G+C content					1046:1056	The genomic DNA G+C content	1030:1056	The genomic DNA G+C content of strain CM134L-2T	1030:1076	The genomic DNA G+C content of strain CM134L-2T was 39.3 mol%, menaquinone-7 was the major respiratory quinone, phosphatidylethanolamine was the major polar lipid and the major components of the cellular fatty acids were iso-C15 : 0, and C16 : 1ω7c/C16 : 1ω6c (summed feature 3); these features supported the affiliation of strain CM134L-2T to the genus Pedobacter.
30307389	7	51	theme	39.3 mol	1082:1089	arg1	%					1090:1090	39.3 mol%	1082:1090	39.3 mol%	1082:1090	The genomic DNA G+C content of strain CM134L-2T was 39.3 mol%, menaquinone-7 was the major respiratory quinone, phosphatidylethanolamine was the major polar lipid and the major components of the cellular fatty acids were iso-C15 : 0, and C16 : 1ω7c/C16 : 1ω6c (summed feature 3); these features supported the affiliation of strain CM134L-2T to the genus Pedobacter.
30307389	6	52	dep	%	846:846	arg1	boundaries					921:930	lower than the proposed and generally accepted species boundaries	866:930	lower than the proposed and generally accepted species boundaries of 70 % for dDDH and 95-96 % for ANI	866:967	Digital DNA-DNA hybridization (dDDH) values between strain CM134L-2T with these two type strains were 26.8 and 20.8 %, respectively, and average nucleotide identity (ANI) values were 83.2 and 76.2 %; these values are lower than the proposed and generally accepted species boundaries of 70 % for dDDH and 95-96 % for ANI, which suggests strain CM134L-2T represents a novel species.
30307389	6	52	dep	%	846:846	arg1	values					855:860	these values	849:860	these values	849:860	Digital DNA-DNA hybridization (dDDH) values between strain CM134L-2T with these two type strains were 26.8 and 20.8 %, respectively, and average nucleotide identity (ANI) values were 83.2 and 76.2 %; these values are lower than the proposed and generally accepted species boundaries of 70 % for dDDH and 95-96 % for ANI, which suggests strain CM134L-2T represents a novel species.
30307389	6	53	theme	novel	1015:1019	arg1	species					1021:1027	a novel species	1013:1027	a novel species	1013:1027	Digital DNA-DNA hybridization (dDDH) values between strain CM134L-2T with these two type strains were 26.8 and 20.8 %, respectively, and average nucleotide identity (ANI) values were 83.2 and 76.2 %; these values are lower than the proposed and generally accepted species boundaries of 70 % for dDDH and 95-96 % for ANI, which suggests strain CM134L-2T represents a novel species.
30307389	3	54	theme	motile	300:305	arg1	yellow					331:336	Gram-stain-negative, strictly aerobic, non-spore-forming, motile, rod-shaped, and bright yellow	242:336	Gram-stain-negative, strictly aerobic, non-spore-forming, motile, rod-shaped, and bright yellow	242:336	It was Gram-stain-negative, strictly aerobic, non-spore-forming, motile, rod-shaped, and bright yellow in colour.
30307389	7	55	theme	strain	1354:1359	arg1	CM134L-2T					1361:1369	strain CM134L-2T	1354:1369	strain CM134L-2T	1354:1369	The genomic DNA G+C content of strain CM134L-2T was 39.3 mol%, menaquinone-7 was the major respiratory quinone, phosphatidylethanolamine was the major polar lipid and the major components of the cellular fatty acids were iso-C15 : 0, and C16 : 1ω7c/C16 : 1ω6c (summed feature 3); these features supported the affiliation of strain CM134L-2T to the genus Pedobacter.
30307389	5	56	theme	strain	514:519	arg1	CM134L-2T					521:529	strain CM134L-2T	514:529	strain CM134L-2T	514:529	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CM134L-2T was most closely related to Pedobacter nanyangensis Q-4T (97.7 %) and Pedobacter zeaxanthinifaciens TDMA-5T (97.4 %).
30307389	2	57	attach	isolated	144:151	arg2	bacterium					100:108	A bacterium	98:108	A bacterium	98:108	A bacterium, designated strain CM134L-2T, was isolated from a chitin-enriched wheat leaf microbiome in Chengdu, Sichuan province, China.
30307389	2	57	attach	isolated	144:151	arg1	microbiome					187:196	a chitin-enriched wheat leaf microbiome	158:196	a chitin-enriched wheat leaf microbiome in Chengdu, Sichuan province, China	158:232	A bacterium, designated strain CM134L-2T, was isolated from a chitin-enriched wheat leaf microbiome in Chengdu, Sichuan province, China.
30307389	8	58	theme	novel	1482:1486	arg1	species					1488:1494	a novel species	1480:1494	a novel species	1480:1494	Overall, strain CM134L-2T belongs to the genus Pedobacter, but can be classified as a novel species, for which the name Pedobacter chitinilyticus sp.
30307389	8	58	theme	novel	1482:1486	arg1	CM134L-2T					1412:1420	strain CM134L-2T	1405:1420	strain CM134L-2T	1405:1420	Overall, strain CM134L-2T belongs to the genus Pedobacter, but can be classified as a novel species, for which the name Pedobacter chitinilyticus sp.
30307389	7	59	theme	fatty	1234:1238	arg1	acids					1240:1244	the cellular fatty acids	1221:1244	the cellular fatty acids	1221:1244	The genomic DNA G+C content of strain CM134L-2T was 39.3 mol%, menaquinone-7 was the major respiratory quinone, phosphatidylethanolamine was the major polar lipid and the major components of the cellular fatty acids were iso-C15 : 0, and C16 : 1ω7c/C16 : 1ω6c (summed feature 3); these features supported the affiliation of strain CM134L-2T to the genus Pedobacter.
30307389	7	60	theme	CM134L-2T	1361:1369	arg1	affiliation					1339:1349	the affiliation	1335:1349	the affiliation of strain CM134L-2T to the genus Pedobacter	1335:1393	The genomic DNA G+C content of strain CM134L-2T was 39.3 mol%, menaquinone-7 was the major respiratory quinone, phosphatidylethanolamine was the major polar lipid and the major components of the cellular fatty acids were iso-C15 : 0, and C16 : 1ω7c/C16 : 1ω6c (summed feature 3); these features supported the affiliation of strain CM134L-2T to the genus Pedobacter.
30307389	7	61	theme	acids	1240:1244	arg1	components					1207:1216	the major components	1197:1216	the major components of the cellular fatty acids	1197:1244	The genomic DNA G+C content of strain CM134L-2T was 39.3 mol%, menaquinone-7 was the major respiratory quinone, phosphatidylethanolamine was the major polar lipid and the major components of the cellular fatty acids were iso-C15 : 0, and C16 : 1ω7c/C16 : 1ω6c (summed feature 3); these features supported the affiliation of strain CM134L-2T to the genus Pedobacter.
30307389	7	61	theme	acids	1240:1244	arg1	iso-C15 					1251:1258	iso-C15 	1251:1258	iso-C15 	1251:1258	The genomic DNA G+C content of strain CM134L-2T was 39.3 mol%, menaquinone-7 was the major respiratory quinone, phosphatidylethanolamine was the major polar lipid and the major components of the cellular fatty acids were iso-C15 : 0, and C16 : 1ω7c/C16 : 1ω6c (summed feature 3); these features supported the affiliation of strain CM134L-2T to the genus Pedobacter.
30307389	3	62	theme	non-spore-forming	281:297	arg1	yellow					331:336	Gram-stain-negative, strictly aerobic, non-spore-forming, motile, rod-shaped, and bright yellow	242:336	Gram-stain-negative, strictly aerobic, non-spore-forming, motile, rod-shaped, and bright yellow	242:336	It was Gram-stain-negative, strictly aerobic, non-spore-forming, motile, rod-shaped, and bright yellow in colour.
30307389	5	63	theme	16S	478:480	arg1	sequences					492:500	16S rRNA gene sequences	478:500	16S rRNA gene sequences	478:500	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CM134L-2T was most closely related to Pedobacter nanyangensis Q-4T (97.7 %) and Pedobacter zeaxanthinifaciens TDMA-5T (97.4 %).
30307389	8	64	theme	Pedobacter	1516:1525	arg1	sp					1542:1543	the name Pedobacter chitinilyticus sp	1507:1543	the name Pedobacter chitinilyticus sp	1507:1543	Overall, strain CM134L-2T belongs to the genus Pedobacter, but can be classified as a novel species, for which the name Pedobacter chitinilyticus sp.
30307389	8	65	theme	genus	1437:1441	arg1	Pedobacter					1443:1452	the genus Pedobacter	1433:1452	the genus Pedobacter	1433:1452	Overall, strain CM134L-2T belongs to the genus Pedobacter, but can be classified as a novel species, for which the name Pedobacter chitinilyticus sp.
30307389	3	66	dep	Gram-stain-negative	242:260	arg1	aerobic					272:278	aerobic	272:278	aerobic	272:278	It was Gram-stain-negative, strictly aerobic, non-spore-forming, motile, rod-shaped, and bright yellow in colour.
30307389	2	67	theme	leaf	182:185	arg1	microbiome					187:196	a chitin-enriched wheat leaf microbiome	158:196	a chitin-enriched wheat leaf microbiome in Chengdu, Sichuan province, China	158:232	A bacterium, designated strain CM134L-2T, was isolated from a chitin-enriched wheat leaf microbiome in Chengdu, Sichuan province, China.
30307389	7	68	theme	cellular	1225:1232	arg1	acids					1240:1244	the cellular fatty acids	1221:1244	the cellular fatty acids	1221:1244	The genomic DNA G+C content of strain CM134L-2T was 39.3 mol%, menaquinone-7 was the major respiratory quinone, phosphatidylethanolamine was the major polar lipid and the major components of the cellular fatty acids were iso-C15 : 0, and C16 : 1ω7c/C16 : 1ω6c (summed feature 3); these features supported the affiliation of strain CM134L-2T to the genus Pedobacter.
30307389	5	69	theme	rRNA	482:485	arg1	sequences					492:500	16S rRNA gene sequences	478:500	16S rRNA gene sequences	478:500	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CM134L-2T was most closely related to Pedobacter nanyangensis Q-4T (97.7 %) and Pedobacter zeaxanthinifaciens TDMA-5T (97.4 %).
30307389	6	70	theme	strain	985:990	arg1	CM134L-2T					992:1000	strain CM134L-2T	985:1000	strain CM134L-2T	985:1000	Digital DNA-DNA hybridization (dDDH) values between strain CM134L-2T with these two type strains were 26.8 and 20.8 %, respectively, and average nucleotide identity (ANI) values were 83.2 and 76.2 %; these values are lower than the proposed and generally accepted species boundaries of 70 % for dDDH and 95-96 % for ANI, which suggests strain CM134L-2T represents a novel species.
30307389	2	71	theme	strain	122:127	arg1	CM134L-2T					129:137	strain CM134L-2T	122:137	strain CM134L-2T	122:137	A bacterium, designated strain CM134L-2T, was isolated from a chitin-enriched wheat leaf microbiome in Chengdu, Sichuan province, China.
30307389	1	72	theme	chitin-degrading	38:53	arg1	bacterium					55:63	a chitin-degrading bacterium	36:63	a chitin-degrading bacterium	36:63	nov., a chitin-degrading bacterium isolated from wheat leaf tissue.
30307389	1	72	theme	chitin-degrading	38:53	arg1	nov.					30:33	nov.	30:33	nov.	30:33	nov., a chitin-degrading bacterium isolated from wheat leaf tissue.
30307389	5	73	theme	gene	487:490	arg1	sequences					492:500	16S rRNA gene sequences	478:500	16S rRNA gene sequences	478:500	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CM134L-2T was most closely related to Pedobacter nanyangensis Q-4T (97.7 %) and Pedobacter zeaxanthinifaciens TDMA-5T (97.4 %).
30307389	6	74	theme	hybridization	665:677	arg1	values					686:691	Digital DNA-DNA hybridization (dDDH) values	649:691	Digital DNA-DNA hybridization (dDDH) values between strain CM134L-2T with these two type strains	649:744	Digital DNA-DNA hybridization (dDDH) values between strain CM134L-2T with these two type strains were 26.8 and 20.8 %, respectively, and average nucleotide identity (ANI) values were 83.2 and 76.2 %; these values are lower than the proposed and generally accepted species boundaries of 70 % for dDDH and 95-96 % for ANI, which suggests strain CM134L-2T represents a novel species.
30307389	7	75	theme	genus	1378:1382	arg1	Pedobacter					1384:1393	the genus Pedobacter	1374:1393	the genus Pedobacter	1374:1393	The genomic DNA G+C content of strain CM134L-2T was 39.3 mol%, menaquinone-7 was the major respiratory quinone, phosphatidylethanolamine was the major polar lipid and the major components of the cellular fatty acids were iso-C15 : 0, and C16 : 1ω7c/C16 : 1ω6c (summed feature 3); these features supported the affiliation of strain CM134L-2T to the genus Pedobacter.
30121748	2	0	theme	composition	529:539	arg1	levels					591:596	the levels	587:596	the levels of short-chain fatty acids (SCFAs) and ammonium ions (NH4+)	587:656	The effects of the treatments were assessed by the analysis of the intestinal microbial composition (using 16S rRNA gene amplicon sequencing) and the levels of short-chain fatty acids (SCFAs) and ammonium ions (NH4+).
30121748	2	0	theme	composition	529:539	arg1	analysis					492:499	the analysis	488:499	the analysis of the intestinal microbial composition (using 16S rRNA gene amplicon sequencing)	488:581	The effects of the treatments were assessed by the analysis of the intestinal microbial composition (using 16S rRNA gene amplicon sequencing) and the levels of short-chain fatty acids (SCFAs) and ammonium ions (NH4+).
30121748	5	1	theme	genera	1078:1083	arg1	production					958:967	the production	954:967	the production of butyric acid in the simulated transverse and descending colon, reduction of NH4+ as well as the growth of genera Lactobacillus, Megamonas, and members of Lachnospiracea	954:1139	Treatment T1 stimulated the production of butyric acid in the simulated transverse and descending colon, reduction of NH4+ as well as the growth of genera Lactobacillus, Megamonas, and members of Lachnospiracea.
30121748	5	1	theme	genera	1078:1083	arg1	growth					1068:1073	the growth	1064:1073	the production of butyric acid in the simulated transverse and descending colon, reduction of NH4+ as well as the growth of genera Lactobacillus, Megamonas, and members of Lachnospiracea	954:1139	Treatment T1 stimulated the production of butyric acid in the simulated transverse and descending colon, reduction of NH4+ as well as the growth of genera Lactobacillus, Megamonas, and members of Lachnospiracea.
30121748	1	2	dep	BB-46	235:239	arg1	i.e.					206:209	i.e.	206:209	i.e.	206:209	This study aimed to evaluate the effects of three treatments, i.e., Bifidobacterium longum BB-46 (T1), B. longum BB-46 combined with the pectin (T2), and harsh extracted pectin from lemon (T3) on obesity-related microbiota using the Simulator of the Human Intestinal Microbial Ecosystem (SHIME®).
30121748	7	3	theme	citric	1385:1390	arg1	able					1402:1405	able	1402:1405	able	1402:1405	This study showed that the citric pectin is able to stimulate butyrate-producing bacteria as well as genera related with anti-inflammatory effects.
30121748	7	3	theme	citric	1385:1390	arg1	pectin					1392:1397	the citric pectin	1381:1397	the citric pectin	1381:1397	This study showed that the citric pectin is able to stimulate butyrate-producing bacteria as well as genera related with anti-inflammatory effects.
30121748	6	4	dep	modulate	1204:1211	arg1	combined					1273:1280	combined	1273:1280	is combined with B. longum BB-46	1270:1301	The results indicate that both B. longum BB-46 and pectin can modulate the obesity-related microbiota; however, when the pectin is combined with B. longum BB-46, the predominant effect of the pectin can be observed.
30121748	6	4	dep	modulate	1204:1211	arg1	observed					1348:1355	observed	1348:1355	can be observed	1341:1355	The results indicate that both B. longum BB-46 and pectin can modulate the obesity-related microbiota; however, when the pectin is combined with B. longum BB-46, the predominant effect of the pectin can be observed.
30121748	3	5	theme	Succinivibrionaceae	726:744	arg1	families					746:753	the Ruminococcaceae and Succinivibrionaceae families	702:753	the Ruminococcaceae and Succinivibrionaceae families	702:753	Treatments T2 and T3 stimulated members of the Ruminococcaceae and Succinivibrionaceae families, which were positively correlated with an increase in butyric and acetic acids.
30121748	0	6	theme	pectin	87:92	arg1	fermentation					64:75	in vitro fermentation	55:75	in vitro fermentation of citrus pectin in combination with Bifidobacterium longum BB-46	55:141	Modulation of gut microbiota from obese individuals by in vitro fermentation of citrus pectin in combination with Bifidobacterium longum BB-46.
30121748	0	7	from	Modulation	0:9	arg1	individuals					40:50	obese individuals	34:50	obese individuals	34:50	Modulation of gut microbiota from obese individuals by in vitro fermentation of citrus pectin in combination with Bifidobacterium longum BB-46.
30121748	2	8	theme	intestinal	508:517	arg1	composition					529:539	the intestinal microbial composition	504:539	the intestinal microbial composition (using 16S rRNA gene amplicon sequencing)	504:581	The effects of the treatments were assessed by the analysis of the intestinal microbial composition (using 16S rRNA gene amplicon sequencing) and the levels of short-chain fatty acids (SCFAs) and ammonium ions (NH4+).
30121748	5	9	dep	genera	1078:1083	arg1	Megamonas					1100:1108	Megamonas	1100:1108	Megamonas	1100:1108	Treatment T1 stimulated the production of butyric acid in the simulated transverse and descending colon, reduction of NH4+ as well as the growth of genera Lactobacillus, Megamonas, and members of Lachnospiracea.
30121748	5	9	dep	genera	1078:1083	arg1	members					1115:1121	members	1115:1121	members of Lachnospiracea	1115:1139	Treatment T1 stimulated the production of butyric acid in the simulated transverse and descending colon, reduction of NH4+ as well as the growth of genera Lactobacillus, Megamonas, and members of Lachnospiracea.
30121748	5	9	dep	genera	1078:1083	arg1	Lactobacillus					1085:1097	Lactobacillus	1085:1097	Lactobacillus	1085:1097	Treatment T1 stimulated the production of butyric acid in the simulated transverse and descending colon, reduction of NH4+ as well as the growth of genera Lactobacillus, Megamonas, and members of Lachnospiracea.
30121748	0	10	theme	citrus	80:85	arg1	pectin					87:92	citrus pectin	80:92	citrus pectin	80:92	Modulation of gut microbiota from obese individuals by in vitro fermentation of citrus pectin in combination with Bifidobacterium longum BB-46.
30121748	6	11	theme	pectin	1334:1339	arg1	effect					1320:1325	the predominant effect	1304:1325	the predominant effect of the pectin	1304:1339	The results indicate that both B. longum BB-46 and pectin can modulate the obesity-related microbiota; however, when the pectin is combined with B. longum BB-46, the predominant effect of the pectin can be observed.
30121748	2	12	theme	amplicon	562:569	arg1	sequencing					571:580	16S rRNA gene amplicon sequencing	548:580	16S rRNA gene amplicon sequencing	548:580	The effects of the treatments were assessed by the analysis of the intestinal microbial composition (using 16S rRNA gene amplicon sequencing) and the levels of short-chain fatty acids (SCFAs) and ammonium ions (NH4+).
30121748	5	13	theme	butyric	972:978	arg1	acid					980:983	butyric acid	972:983	butyric acid in the simulated transverse and descending colon	972:1032	Treatment T1 stimulated the production of butyric acid in the simulated transverse and descending colon, reduction of NH4+ as well as the growth of genera Lactobacillus, Megamonas, and members of Lachnospiracea.
30121748	5	13	theme	butyric	972:978	arg1	reduction					1035:1043	reduction	1035:1043	reduction of NH4+	1035:1051	Treatment T1 stimulated the production of butyric acid in the simulated transverse and descending colon, reduction of NH4+ as well as the growth of genera Lactobacillus, Megamonas, and members of Lachnospiracea.
30121748	3	14	theme	butyric	809:815	arg1	acids					828:832	butyric and acetic acids	809:832	butyric and acetic acids	809:832	Treatments T2 and T3 stimulated members of the Ruminococcaceae and Succinivibrionaceae families, which were positively correlated with an increase in butyric and acetic acids.
30121748	2	15	theme	ions	646:649	arg1	levels					591:596	the levels	587:596	the levels of short-chain fatty acids (SCFAs) and ammonium ions (NH4+)	587:656	The effects of the treatments were assessed by the analysis of the intestinal microbial composition (using 16S rRNA gene amplicon sequencing) and the levels of short-chain fatty acids (SCFAs) and ammonium ions (NH4+).
30121748	2	15	theme	ions	646:649	arg1	analysis					492:499	the analysis	488:499	the analysis of the intestinal microbial composition (using 16S rRNA gene amplicon sequencing)	488:581	The effects of the treatments were assessed by the analysis of the intestinal microbial composition (using 16S rRNA gene amplicon sequencing) and the levels of short-chain fatty acids (SCFAs) and ammonium ions (NH4+).
30121748	6	16	dep	B.	1173:1174	arg1	longum					1176:1181	longum	1176:1181	longum	1176:1181	The results indicate that both B. longum BB-46 and pectin can modulate the obesity-related microbiota; however, when the pectin is combined with B. longum BB-46, the predominant effect of the pectin can be observed.
30121748	1	17	theme	harsh	298:302	arg1	T3					333:334	T3	333:334	T3	333:334	This study aimed to evaluate the effects of three treatments, i.e., Bifidobacterium longum BB-46 (T1), B. longum BB-46 combined with the pectin (T2), and harsh extracted pectin from lemon (T3) on obesity-related microbiota using the Simulator of the Human Intestinal Microbial Ecosystem (SHIME®).
30121748	1	17	theme	harsh	298:302	arg1	pectin					314:319	harsh extracted pectin	298:319	harsh extracted pectin from lemon (T3)	298:335	This study aimed to evaluate the effects of three treatments, i.e., Bifidobacterium longum BB-46 (T1), B. longum BB-46 combined with the pectin (T2), and harsh extracted pectin from lemon (T3) on obesity-related microbiota using the Simulator of the Human Intestinal Microbial Ecosystem (SHIME®).
30121748	2	18	theme	rRNA	552:555	arg1	sequencing					571:580	16S rRNA gene amplicon sequencing	548:580	16S rRNA gene amplicon sequencing	548:580	The effects of the treatments were assessed by the analysis of the intestinal microbial composition (using 16S rRNA gene amplicon sequencing) and the levels of short-chain fatty acids (SCFAs) and ammonium ions (NH4+).
30121748	2	19	theme	gene	557:560	arg1	sequencing					571:580	16S rRNA gene amplicon sequencing	548:580	16S rRNA gene amplicon sequencing	548:580	The effects of the treatments were assessed by the analysis of the intestinal microbial composition (using 16S rRNA gene amplicon sequencing) and the levels of short-chain fatty acids (SCFAs) and ammonium ions (NH4+).
30121748	8	20	theme	anti/pro-obesogenic	1574:1592	arg1	effects					1611:1617	the anti/pro-obesogenic and inflammatory effects	1570:1617	the anti/pro-obesogenic and inflammatory effects of this pectin for future prevention of obesity	1570:1665	However, prospective clinical studies are necessary to evaluate the anti/pro-obesogenic and inflammatory effects of this pectin for future prevention of obesity.
30121748	7	21	theme	anti-inflammatory	1479:1495	arg1	effects					1497:1503	anti-inflammatory effects	1479:1503	anti-inflammatory effects	1479:1503	This study showed that the citric pectin is able to stimulate butyrate-producing bacteria as well as genera related with anti-inflammatory effects.
30121748	1	22	from	lemon	326:330	arg1	T3					333:334	T3	333:334	T3	333:334	This study aimed to evaluate the effects of three treatments, i.e., Bifidobacterium longum BB-46 (T1), B. longum BB-46 combined with the pectin (T2), and harsh extracted pectin from lemon (T3) on obesity-related microbiota using the Simulator of the Human Intestinal Microbial Ecosystem (SHIME®).
30121748	1	22	from	lemon	326:330	arg1	BB-46					257:261	B. longum BB-46	247:261	B. longum BB-46 combined with the pectin (T2)	247:291	This study aimed to evaluate the effects of three treatments, i.e., Bifidobacterium longum BB-46 (T1), B. longum BB-46 combined with the pectin (T2), and harsh extracted pectin from lemon (T3) on obesity-related microbiota using the Simulator of the Human Intestinal Microbial Ecosystem (SHIME®).
30121748	1	22	from	lemon	326:330	arg1	T1					242:243	T1	242:243	T1	242:243	This study aimed to evaluate the effects of three treatments, i.e., Bifidobacterium longum BB-46 (T1), B. longum BB-46 combined with the pectin (T2), and harsh extracted pectin from lemon (T3) on obesity-related microbiota using the Simulator of the Human Intestinal Microbial Ecosystem (SHIME®).
30121748	1	22	from	lemon	326:330	arg1	BB-46					235:239	Bifidobacterium longum BB-46	212:239	Bifidobacterium longum BB-46 (T1)	212:244	This study aimed to evaluate the effects of three treatments, i.e., Bifidobacterium longum BB-46 (T1), B. longum BB-46 combined with the pectin (T2), and harsh extracted pectin from lemon (T3) on obesity-related microbiota using the Simulator of the Human Intestinal Microbial Ecosystem (SHIME®).
30121748	1	22	from	lemon	326:330	arg1	pectin					314:319	harsh extracted pectin	298:319	harsh extracted pectin from lemon (T3)	298:335	This study aimed to evaluate the effects of three treatments, i.e., Bifidobacterium longum BB-46 (T1), B. longum BB-46 combined with the pectin (T2), and harsh extracted pectin from lemon (T3) on obesity-related microbiota using the Simulator of the Human Intestinal Microbial Ecosystem (SHIME®).
30121748	1	23	theme	extracted	304:312	arg1	T3					333:334	T3	333:334	T3	333:334	This study aimed to evaluate the effects of three treatments, i.e., Bifidobacterium longum BB-46 (T1), B. longum BB-46 combined with the pectin (T2), and harsh extracted pectin from lemon (T3) on obesity-related microbiota using the Simulator of the Human Intestinal Microbial Ecosystem (SHIME®).
30121748	1	23	theme	extracted	304:312	arg1	pectin					314:319	harsh extracted pectin	298:319	harsh extracted pectin from lemon (T3)	298:335	This study aimed to evaluate the effects of three treatments, i.e., Bifidobacterium longum BB-46 (T1), B. longum BB-46 combined with the pectin (T2), and harsh extracted pectin from lemon (T3) on obesity-related microbiota using the Simulator of the Human Intestinal Microbial Ecosystem (SHIME®).
30121748	2	24	theme	16S	548:550	arg1	sequencing					571:580	16S rRNA gene amplicon sequencing	548:580	16S rRNA gene amplicon sequencing	548:580	The effects of the treatments were assessed by the analysis of the intestinal microbial composition (using 16S rRNA gene amplicon sequencing) and the levels of short-chain fatty acids (SCFAs) and ammonium ions (NH4+).
30121748	0	25	with	combination	97:107	arg1	BB-46					137:141	BB-46	137:141	BB-46	137:141	Modulation of gut microbiota from obese individuals by in vitro fermentation of citrus pectin in combination with Bifidobacterium longum BB-46.
30121748	3	26	dep	Treatments	659:668	arg1	Treatments					659:668	Treatments T2 and T3	659:678	Treatments T2 and T3	659:678	Treatments T2 and T3 stimulated members of the Ruminococcaceae and Succinivibrionaceae families, which were positively correlated with an increase in butyric and acetic acids.
30121748	3	26	dep	Treatments	659:668	arg1	T3					677:678	T3	677:678	T3	677:678	Treatments T2 and T3 stimulated members of the Ruminococcaceae and Succinivibrionaceae families, which were positively correlated with an increase in butyric and acetic acids.
30121748	3	26	dep	Treatments	659:668	arg1	T2					670:671	T2	670:671	T2	670:671	Treatments T2 and T3 stimulated members of the Ruminococcaceae and Succinivibrionaceae families, which were positively correlated with an increase in butyric and acetic acids.
30121748	5	27	theme	simulated	992:1000	arg1	transverse					1002:1011	the simulated transverse and descending colon	988:1032	transverse	1002:1011	Treatment T1 stimulated the production of butyric acid in the simulated transverse and descending colon, reduction of NH4+ as well as the growth of genera Lactobacillus, Megamonas, and members of Lachnospiracea.
30121748	5	28	from	transverse	1002:1011	arg1	production					958:967	the production	954:967	the production of butyric acid in the simulated transverse and descending colon, reduction of NH4+ as well as the growth of genera Lactobacillus, Megamonas, and members of Lachnospiracea	954:1139	Treatment T1 stimulated the production of butyric acid in the simulated transverse and descending colon, reduction of NH4+ as well as the growth of genera Lactobacillus, Megamonas, and members of Lachnospiracea.
30121748	5	28	from	transverse	1002:1011	arg1	growth					1068:1073	the growth	1064:1073	the production of butyric acid in the simulated transverse and descending colon, reduction of NH4+ as well as the growth of genera Lactobacillus, Megamonas, and members of Lachnospiracea	954:1139	Treatment T1 stimulated the production of butyric acid in the simulated transverse and descending colon, reduction of NH4+ as well as the growth of genera Lactobacillus, Megamonas, and members of Lachnospiracea.
30121748	1	29	theme	Human	394:398	arg1	SHIME®					432:437	SHIME®	432:437	SHIME®	432:437	This study aimed to evaluate the effects of three treatments, i.e., Bifidobacterium longum BB-46 (T1), B. longum BB-46 combined with the pectin (T2), and harsh extracted pectin from lemon (T3) on obesity-related microbiota using the Simulator of the Human Intestinal Microbial Ecosystem (SHIME®).
30121748	1	29	theme	Human	394:398	arg1	Ecosystem					421:429	the Human Intestinal Microbial Ecosystem	390:429	the Human Intestinal Microbial Ecosystem (SHIME®)	390:438	This study aimed to evaluate the effects of three treatments, i.e., Bifidobacterium longum BB-46 (T1), B. longum BB-46 combined with the pectin (T2), and harsh extracted pectin from lemon (T3) on obesity-related microbiota using the Simulator of the Human Intestinal Microbial Ecosystem (SHIME®).
30121748	0	30	from	individuals	40:50	arg1	Modulation					0:9	Modulation	0:9	Modulation of gut microbiota from obese individuals by in vitro fermentation of citrus pectin in combination with Bifidobacterium longum BB-46.	0:142	Modulation of gut microbiota from obese individuals by in vitro fermentation of citrus pectin in combination with Bifidobacterium longum BB-46.
30121748	0	30	from	individuals	40:50	arg1	microbiota					18:27	gut microbiota	14:27	gut microbiota from obese individuals	14:50	Modulation of gut microbiota from obese individuals by in vitro fermentation of citrus pectin in combination with Bifidobacterium longum BB-46.
30121748	4	31	theme	Proteolytic	835:845	arg1	bacteria					847:854	Proteolytic bacteria	835:854	Proteolytic bacteria	835:854	Proteolytic bacteria were reduced by the two treatments, concurrently with a decrease in NH4+.
30121748	1	32	theme	Intestinal	400:409	arg1	SHIME®					432:437	SHIME®	432:437	SHIME®	432:437	This study aimed to evaluate the effects of three treatments, i.e., Bifidobacterium longum BB-46 (T1), B. longum BB-46 combined with the pectin (T2), and harsh extracted pectin from lemon (T3) on obesity-related microbiota using the Simulator of the Human Intestinal Microbial Ecosystem (SHIME®).
30121748	1	32	theme	Intestinal	400:409	arg1	Ecosystem					421:429	the Human Intestinal Microbial Ecosystem	390:429	the Human Intestinal Microbial Ecosystem (SHIME®)	390:438	This study aimed to evaluate the effects of three treatments, i.e., Bifidobacterium longum BB-46 (T1), B. longum BB-46 combined with the pectin (T2), and harsh extracted pectin from lemon (T3) on obesity-related microbiota using the Simulator of the Human Intestinal Microbial Ecosystem (SHIME®).
30121748	5	33	from	growth	1068:1073	arg1	transverse					1002:1011	the simulated transverse and descending colon	988:1032	transverse	1002:1011	Treatment T1 stimulated the production of butyric acid in the simulated transverse and descending colon, reduction of NH4+ as well as the growth of genera Lactobacillus, Megamonas, and members of Lachnospiracea.
30121748	5	33	from	growth	1068:1073	arg1	colon					1028:1032	the simulated transverse and descending colon	988:1032	colon	1028:1032	Treatment T1 stimulated the production of butyric acid in the simulated transverse and descending colon, reduction of NH4+ as well as the growth of genera Lactobacillus, Megamonas, and members of Lachnospiracea.
30121748	2	34	theme	microbial	519:527	arg1	composition					529:539	the intestinal microbial composition	504:539	the intestinal microbial composition (using 16S rRNA gene amplicon sequencing)	504:581	The effects of the treatments were assessed by the analysis of the intestinal microbial composition (using 16S rRNA gene amplicon sequencing) and the levels of short-chain fatty acids (SCFAs) and ammonium ions (NH4+).
30121748	0	35	theme	microbiota	18:27	arg1	Modulation					0:9	Modulation	0:9	Modulation of gut microbiota from obese individuals by in vitro fermentation of citrus pectin in combination with Bifidobacterium longum BB-46.	0:142	Modulation of gut microbiota from obese individuals by in vitro fermentation of citrus pectin in combination with Bifidobacterium longum BB-46.
30121748	1	36	theme	B.	247:248	arg1	BB-46					257:261	B. longum BB-46	247:261	B. longum BB-46 combined with the pectin (T2)	247:291	This study aimed to evaluate the effects of three treatments, i.e., Bifidobacterium longum BB-46 (T1), B. longum BB-46 combined with the pectin (T2), and harsh extracted pectin from lemon (T3) on obesity-related microbiota using the Simulator of the Human Intestinal Microbial Ecosystem (SHIME®).
30121748	6	37	theme	obesity-related	1217:1231	arg1	microbiota					1233:1242	the obesity-related microbiota	1213:1242	the obesity-related microbiota	1213:1242	The results indicate that both B. longum BB-46 and pectin can modulate the obesity-related microbiota; however, when the pectin is combined with B. longum BB-46, the predominant effect of the pectin can be observed.
30121748	1	38	theme	Microbial	411:419	arg1	SHIME®					432:437	SHIME®	432:437	SHIME®	432:437	This study aimed to evaluate the effects of three treatments, i.e., Bifidobacterium longum BB-46 (T1), B. longum BB-46 combined with the pectin (T2), and harsh extracted pectin from lemon (T3) on obesity-related microbiota using the Simulator of the Human Intestinal Microbial Ecosystem (SHIME®).
30121748	1	38	theme	Microbial	411:419	arg1	Ecosystem					421:429	the Human Intestinal Microbial Ecosystem	390:429	the Human Intestinal Microbial Ecosystem (SHIME®)	390:438	This study aimed to evaluate the effects of three treatments, i.e., Bifidobacterium longum BB-46 (T1), B. longum BB-46 combined with the pectin (T2), and harsh extracted pectin from lemon (T3) on obesity-related microbiota using the Simulator of the Human Intestinal Microbial Ecosystem (SHIME®).
30121748	3	39	theme	Ruminococcaceae	706:720	arg1	families					746:753	the Ruminococcaceae and Succinivibrionaceae families	702:753	the Ruminococcaceae and Succinivibrionaceae families	702:753	Treatments T2 and T3 stimulated members of the Ruminococcaceae and Succinivibrionaceae families, which were positively correlated with an increase in butyric and acetic acids.
30121748	0	40	theme	gut	14:16	arg1	microbiota					18:27	gut microbiota	14:27	gut microbiota from obese individuals	14:50	Modulation of gut microbiota from obese individuals by in vitro fermentation of citrus pectin in combination with Bifidobacterium longum BB-46.
30121748	7	41	theme	butyrate-producing	1420:1437	arg1	bacteria					1439:1446	butyrate-producing bacteria	1420:1446	butyrate-producing bacteria as well as genera related with anti-inflammatory effects	1420:1503	This study showed that the citric pectin is able to stimulate butyrate-producing bacteria as well as genera related with anti-inflammatory effects.
30121748	8	42	theme	obesity	1659:1665	arg1	prevention					1645:1654	future prevention	1638:1654	future prevention of obesity	1638:1665	However, prospective clinical studies are necessary to evaluate the anti/pro-obesogenic and inflammatory effects of this pectin for future prevention of obesity.
30121748	0	43	dep	in	55:56	arg1	vitro					58:62	vitro	58:62	vitro	58:62	Modulation of gut microbiota from obese individuals by in vitro fermentation of citrus pectin in combination with Bifidobacterium longum BB-46.
30121748	3	44	theme	acetic	821:826	arg1	acids					828:832	butyric and acetic acids	809:832	butyric and acetic acids	809:832	Treatments T2 and T3 stimulated members of the Ruminococcaceae and Succinivibrionaceae families, which were positively correlated with an increase in butyric and acetic acids.
30121748	0	45	theme	obese	34:38	arg1	individuals					40:50	obese individuals	34:50	obese individuals	34:50	Modulation of gut microbiota from obese individuals by in vitro fermentation of citrus pectin in combination with Bifidobacterium longum BB-46.
30121748	2	46	theme	treatments	460:469	arg1	effects					445:451	The effects	441:451	The effects of the treatments	441:469	The effects of the treatments were assessed by the analysis of the intestinal microbial composition (using 16S rRNA gene amplicon sequencing) and the levels of short-chain fatty acids (SCFAs) and ammonium ions (NH4+).
30121748	0	47	from	fermentation	64:75	arg1	combination					97:107	combination	97:107	combination with Bifidobacterium longum BB-46	97:141	Modulation of gut microbiota from obese individuals by in vitro fermentation of citrus pectin in combination with Bifidobacterium longum BB-46.
30121748	6	48	theme	B.	1173:1174	arg1	BB-46					1183:1187	B. longum BB-46	1173:1187	B. longum BB-46	1173:1187	The results indicate that both B. longum BB-46 and pectin can modulate the obesity-related microbiota; however, when the pectin is combined with B. longum BB-46, the predominant effect of the pectin can be observed.
30121748	8	49	theme	pectin	1627:1632	arg1	effects					1611:1617	the anti/pro-obesogenic and inflammatory effects	1570:1617	the anti/pro-obesogenic and inflammatory effects of this pectin for future prevention of obesity	1570:1665	However, prospective clinical studies are necessary to evaluate the anti/pro-obesogenic and inflammatory effects of this pectin for future prevention of obesity.
30121748	1	50	theme	treatments	194:203	arg1	effects					177:183	the effects	173:183	the effects of three treatments, i.e., Bifidobacterium longum BB-46 (T1), B. longum BB-46 combined with the pectin (T2), and harsh extracted pectin from lemon (T3) on obesity-related microbiota using the Simulator of the Human Intestinal Microbial Ecosystem (SHIME®)	173:438	This study aimed to evaluate the effects of three treatments, i.e., Bifidobacterium longum BB-46 (T1), B. longum BB-46 combined with the pectin (T2), and harsh extracted pectin from lemon (T3) on obesity-related microbiota using the Simulator of the Human Intestinal Microbial Ecosystem (SHIME®).
30121748	6	51	theme	B.	1287:1288	arg1	BB-46					1297:1301	B. longum BB-46	1287:1301	B. longum BB-46	1287:1301	The results indicate that both B. longum BB-46 and pectin can modulate the obesity-related microbiota; however, when the pectin is combined with B. longum BB-46, the predominant effect of the pectin can be observed.
30121748	4	52	from	decrease	912:919	arg1	NH4+					924:927	NH4+	924:927	NH4+	924:927	Proteolytic bacteria were reduced by the two treatments, concurrently with a decrease in NH4+.
30121748	6	53	dep	B.	1287:1288	arg1	longum					1290:1295	longum	1290:1295	longum	1290:1295	The results indicate that both B. longum BB-46 and pectin can modulate the obesity-related microbiota; however, when the pectin is combined with B. longum BB-46, the predominant effect of the pectin can be observed.
30121748	5	54	theme	acid	980:983	arg1	production					958:967	the production	954:967	the production of butyric acid in the simulated transverse and descending colon, reduction of NH4+ as well as the growth of genera Lactobacillus, Megamonas, and members of Lachnospiracea	954:1139	Treatment T1 stimulated the production of butyric acid in the simulated transverse and descending colon, reduction of NH4+ as well as the growth of genera Lactobacillus, Megamonas, and members of Lachnospiracea.
30121748	5	54	theme	acid	980:983	arg1	growth					1068:1073	the growth	1064:1073	the production of butyric acid in the simulated transverse and descending colon, reduction of NH4+ as well as the growth of genera Lactobacillus, Megamonas, and members of Lachnospiracea	954:1139	Treatment T1 stimulated the production of butyric acid in the simulated transverse and descending colon, reduction of NH4+ as well as the growth of genera Lactobacillus, Megamonas, and members of Lachnospiracea.
30121748	1	55	theme	Ecosystem	421:429	arg1	Simulator					377:385	the Simulator	373:385	the Simulator of the Human Intestinal Microbial Ecosystem (SHIME®)	373:438	This study aimed to evaluate the effects of three treatments, i.e., Bifidobacterium longum BB-46 (T1), B. longum BB-46 combined with the pectin (T2), and harsh extracted pectin from lemon (T3) on obesity-related microbiota using the Simulator of the Human Intestinal Microbial Ecosystem (SHIME®).
30121748	5	56	theme	NH4+	1048:1051	arg1	acid					980:983	butyric acid	972:983	butyric acid in the simulated transverse and descending colon	972:1032	Treatment T1 stimulated the production of butyric acid in the simulated transverse and descending colon, reduction of NH4+ as well as the growth of genera Lactobacillus, Megamonas, and members of Lachnospiracea.
30121748	5	56	theme	NH4+	1048:1051	arg1	reduction					1035:1043	reduction	1035:1043	reduction of NH4+	1035:1051	Treatment T1 stimulated the production of butyric acid in the simulated transverse and descending colon, reduction of NH4+ as well as the growth of genera Lactobacillus, Megamonas, and members of Lachnospiracea.
30121748	5	57	from	acid	980:983	arg1	transverse					1002:1011	the simulated transverse and descending colon	988:1032	transverse	1002:1011	Treatment T1 stimulated the production of butyric acid in the simulated transverse and descending colon, reduction of NH4+ as well as the growth of genera Lactobacillus, Megamonas, and members of Lachnospiracea.
30121748	5	57	from	acid	980:983	arg1	colon					1028:1032	the simulated transverse and descending colon	988:1032	colon	1028:1032	Treatment T1 stimulated the production of butyric acid in the simulated transverse and descending colon, reduction of NH4+ as well as the growth of genera Lactobacillus, Megamonas, and members of Lachnospiracea.
30121748	5	58	from	production	958:967	arg1	transverse					1002:1011	the simulated transverse and descending colon	988:1032	transverse	1002:1011	Treatment T1 stimulated the production of butyric acid in the simulated transverse and descending colon, reduction of NH4+ as well as the growth of genera Lactobacillus, Megamonas, and members of Lachnospiracea.
30121748	5	58	from	production	958:967	arg1	colon					1028:1032	the simulated transverse and descending colon	988:1032	colon	1028:1032	Treatment T1 stimulated the production of butyric acid in the simulated transverse and descending colon, reduction of NH4+ as well as the growth of genera Lactobacillus, Megamonas, and members of Lachnospiracea.
30121748	2	59	theme	acids	619:623	arg1	levels					591:596	the levels	587:596	the levels of short-chain fatty acids (SCFAs) and ammonium ions (NH4+)	587:656	The effects of the treatments were assessed by the analysis of the intestinal microbial composition (using 16S rRNA gene amplicon sequencing) and the levels of short-chain fatty acids (SCFAs) and ammonium ions (NH4+).
30121748	2	59	theme	acids	619:623	arg1	analysis					492:499	the analysis	488:499	the analysis of the intestinal microbial composition (using 16S rRNA gene amplicon sequencing)	488:581	The effects of the treatments were assessed by the analysis of the intestinal microbial composition (using 16S rRNA gene amplicon sequencing) and the levels of short-chain fatty acids (SCFAs) and ammonium ions (NH4+).
30121748	1	60	dep	B.	247:248	arg1	longum					250:255	longum	250:255	longum	250:255	This study aimed to evaluate the effects of three treatments, i.e., Bifidobacterium longum BB-46 (T1), B. longum BB-46 combined with the pectin (T2), and harsh extracted pectin from lemon (T3) on obesity-related microbiota using the Simulator of the Human Intestinal Microbial Ecosystem (SHIME®).
30121748	5	61	theme	Treatment	930:938	arg1	T1					940:941	Treatment T1	930:941	Treatment T1	930:941	Treatment T1 stimulated the production of butyric acid in the simulated transverse and descending colon, reduction of NH4+ as well as the growth of genera Lactobacillus, Megamonas, and members of Lachnospiracea.
30121748	2	62	theme	fatty	613:617	arg1	acids					619:623	short-chain fatty acids	601:623	short-chain fatty acids (SCFAs)	601:631	The effects of the treatments were assessed by the analysis of the intestinal microbial composition (using 16S rRNA gene amplicon sequencing) and the levels of short-chain fatty acids (SCFAs) and ammonium ions (NH4+).
30121748	2	62	theme	fatty	613:617	arg1	SCFAs					626:630	SCFAs	626:630	SCFAs	626:630	The effects of the treatments were assessed by the analysis of the intestinal microbial composition (using 16S rRNA gene amplicon sequencing) and the levels of short-chain fatty acids (SCFAs) and ammonium ions (NH4+).
30121748	8	63	theme	inflammatory	1598:1609	arg1	effects					1611:1617	the anti/pro-obesogenic and inflammatory effects	1570:1617	the anti/pro-obesogenic and inflammatory effects of this pectin for future prevention of obesity	1570:1665	However, prospective clinical studies are necessary to evaluate the anti/pro-obesogenic and inflammatory effects of this pectin for future prevention of obesity.
30121748	8	64	theme	clinical	1527:1534	arg1	studies					1536:1542	prospective clinical studies	1515:1542	prospective clinical studies	1515:1542	However, prospective clinical studies are necessary to evaluate the anti/pro-obesogenic and inflammatory effects of this pectin for future prevention of obesity.
30121748	3	65	from	increase	797:804	arg1	acids					828:832	butyric and acetic acids	809:832	butyric and acetic acids	809:832	Treatments T2 and T3 stimulated members of the Ruminococcaceae and Succinivibrionaceae families, which were positively correlated with an increase in butyric and acetic acids.
30121748	2	66	theme	short-chain	601:611	arg1	acids					619:623	short-chain fatty acids	601:623	short-chain fatty acids (SCFAs)	601:631	The effects of the treatments were assessed by the analysis of the intestinal microbial composition (using 16S rRNA gene amplicon sequencing) and the levels of short-chain fatty acids (SCFAs) and ammonium ions (NH4+).
30121748	2	66	theme	short-chain	601:611	arg1	SCFAs					626:630	SCFAs	626:630	SCFAs	626:630	The effects of the treatments were assessed by the analysis of the intestinal microbial composition (using 16S rRNA gene amplicon sequencing) and the levels of short-chain fatty acids (SCFAs) and ammonium ions (NH4+).
30121748	8	67	theme	prospective	1515:1525	arg1	studies					1536:1542	prospective clinical studies	1515:1542	prospective clinical studies	1515:1542	However, prospective clinical studies are necessary to evaluate the anti/pro-obesogenic and inflammatory effects of this pectin for future prevention of obesity.
30121748	1	68	theme	obesity-related	340:354	arg1	microbiota					356:365	obesity-related microbiota	340:365	obesity-related microbiota using the Simulator of the Human Intestinal Microbial Ecosystem (SHIME®)	340:438	This study aimed to evaluate the effects of three treatments, i.e., Bifidobacterium longum BB-46 (T1), B. longum BB-46 combined with the pectin (T2), and harsh extracted pectin from lemon (T3) on obesity-related microbiota using the Simulator of the Human Intestinal Microbial Ecosystem (SHIME®).
30121748	2	69	theme	ammonium	637:644	arg1	NH4+					652:655	NH4+	652:655	NH4+	652:655	The effects of the treatments were assessed by the analysis of the intestinal microbial composition (using 16S rRNA gene amplicon sequencing) and the levels of short-chain fatty acids (SCFAs) and ammonium ions (NH4+).
30121748	2	69	theme	ammonium	637:644	arg1	ions					646:649	ammonium ions	637:649	ammonium ions (NH4+)	637:656	The effects of the treatments were assessed by the analysis of the intestinal microbial composition (using 16S rRNA gene amplicon sequencing) and the levels of short-chain fatty acids (SCFAs) and ammonium ions (NH4+).
30121748	3	70	theme	families	746:753	arg1	members					691:697	members	691:697	members	691:697	Treatments T2 and T3 stimulated members of the Ruminococcaceae and Succinivibrionaceae families, which were positively correlated with an increase in butyric and acetic acids.
30121748	1	71	from	effects	177:183	arg1	microbiota					356:365	obesity-related microbiota	340:365	obesity-related microbiota using the Simulator of the Human Intestinal Microbial Ecosystem (SHIME®)	340:438	This study aimed to evaluate the effects of three treatments, i.e., Bifidobacterium longum BB-46 (T1), B. longum BB-46 combined with the pectin (T2), and harsh extracted pectin from lemon (T3) on obesity-related microbiota using the Simulator of the Human Intestinal Microbial Ecosystem (SHIME®).
30121748	5	72	from	colon	1028:1032	arg1	production					958:967	the production	954:967	the production of butyric acid in the simulated transverse and descending colon, reduction of NH4+ as well as the growth of genera Lactobacillus, Megamonas, and members of Lachnospiracea	954:1139	Treatment T1 stimulated the production of butyric acid in the simulated transverse and descending colon, reduction of NH4+ as well as the growth of genera Lactobacillus, Megamonas, and members of Lachnospiracea.
30121748	5	72	from	colon	1028:1032	arg1	growth					1068:1073	the growth	1064:1073	the production of butyric acid in the simulated transverse and descending colon, reduction of NH4+ as well as the growth of genera Lactobacillus, Megamonas, and members of Lachnospiracea	954:1139	Treatment T1 stimulated the production of butyric acid in the simulated transverse and descending colon, reduction of NH4+ as well as the growth of genera Lactobacillus, Megamonas, and members of Lachnospiracea.
30121748	8	73	theme	future	1638:1643	arg1	prevention					1645:1654	future prevention	1638:1654	future prevention of obesity	1638:1665	However, prospective clinical studies are necessary to evaluate the anti/pro-obesogenic and inflammatory effects of this pectin for future prevention of obesity.
30121748	0	74	theme	in	55:56	arg1	fermentation					64:75	in vitro fermentation	55:75	in vitro fermentation of citrus pectin in combination with Bifidobacterium longum BB-46	55:141	Modulation of gut microbiota from obese individuals by in vitro fermentation of citrus pectin in combination with Bifidobacterium longum BB-46.
30121748	5	75	theme	descending	1017:1026	arg1	colon					1028:1032	the simulated transverse and descending colon	988:1032	colon	1028:1032	Treatment T1 stimulated the production of butyric acid in the simulated transverse and descending colon, reduction of NH4+ as well as the growth of genera Lactobacillus, Megamonas, and members of Lachnospiracea.
30121748	6	76	theme	predominant	1308:1318	arg1	effect					1320:1325	the predominant effect	1304:1325	the predominant effect of the pectin	1304:1339	The results indicate that both B. longum BB-46 and pectin can modulate the obesity-related microbiota; however, when the pectin is combined with B. longum BB-46, the predominant effect of the pectin can be observed.
30121748	5	77	theme	Lachnospiracea	1126:1139	arg1	Megamonas					1100:1108	Megamonas	1100:1108	Megamonas	1100:1108	Treatment T1 stimulated the production of butyric acid in the simulated transverse and descending colon, reduction of NH4+ as well as the growth of genera Lactobacillus, Megamonas, and members of Lachnospiracea.
30121748	5	77	theme	Lachnospiracea	1126:1139	arg1	members					1115:1121	members	1115:1121	members of Lachnospiracea	1115:1139	Treatment T1 stimulated the production of butyric acid in the simulated transverse and descending colon, reduction of NH4+ as well as the growth of genera Lactobacillus, Megamonas, and members of Lachnospiracea.
30121748	5	77	theme	Lachnospiracea	1126:1139	arg1	Lactobacillus					1085:1097	Lactobacillus	1085:1097	Lactobacillus	1085:1097	Treatment T1 stimulated the production of butyric acid in the simulated transverse and descending colon, reduction of NH4+ as well as the growth of genera Lactobacillus, Megamonas, and members of Lachnospiracea.
30121748	1	78	theme	longum	228:233	arg1	T1					242:243	T1	242:243	T1	242:243	This study aimed to evaluate the effects of three treatments, i.e., Bifidobacterium longum BB-46 (T1), B. longum BB-46 combined with the pectin (T2), and harsh extracted pectin from lemon (T3) on obesity-related microbiota using the Simulator of the Human Intestinal Microbial Ecosystem (SHIME®).
30121748	1	78	theme	longum	228:233	arg1	BB-46					235:239	Bifidobacterium longum BB-46	212:239	Bifidobacterium longum BB-46 (T1)	212:244	This study aimed to evaluate the effects of three treatments, i.e., Bifidobacterium longum BB-46 (T1), B. longum BB-46 combined with the pectin (T2), and harsh extracted pectin from lemon (T3) on obesity-related microbiota using the Simulator of the Human Intestinal Microbial Ecosystem (SHIME®).
31644419	5	0	theme	JCM	629:631	arg1	5805T					633:637	lactis JCM 5805T	622:637	lactis JCM 5805T (97.3 %)	622:646	lactis JCM 5805T (97.3 %) and L. lactissubsp.
31644419	5	0	theme	JCM	629:631	arg1	%					645:645	97.3 %	640:645	97.3 %	640:645	lactis JCM 5805T (97.3 %) and L. lactissubsp.
31644419	4	1	theme	1JSPR-7T	562:569	arg1	sequence					543:550	The 16S rRNA gene sequence	525:550	The 16S rRNA gene sequence of strain 1JSPR-7T	525:569	The 16S rRNA gene sequence of strain 1JSPR-7T revealed the highest homology with L. lactissubsp.
31644419	9	2	theme	average	853:859	arg1	identity					872:879	average nucleotide identity	853:879	average nucleotide identity	853:879	The average nucleotide identity and digital DNA-DNA hybridization values indicated that strain 1JSPR-7T was a novel species of the genus Lacococcus.
31644419	3	3	theme	Lactococcus	500:510	arg1	taiwanensis					512:522	Lactococcus taiwanensis	500:522	Lactococcus taiwanensis	500:522	Comparative analysis of 16S rRNA gene and rpoB gene sequences showed that strain 1JSPR-7T fell within the genus Lactococcus, forming a compact cluster with the type strain of four subspecies of Lactococcus lactis and Lactococcus taiwanensis.
31644419	7	4	theme	cremoris	776:783	arg1	20069T					789:794	cremoris DSM 20069T	776:794	cremoris DSM 20069T (91.4 %)	776:803	cremoris DSM 20069T (91.4 %) and L. lactissubsp.
31644419	7	4	theme	cremoris	776:783	arg1	%					802:802	91.4 %	797:802	91.4 %	797:802	cremoris DSM 20069T (91.4 %) and L. lactissubsp.
31644419	9	5	theme	identity	872:879	arg1	values					915:920	The average nucleotide identity and digital DNA-DNA hybridization values	849:920	The average nucleotide identity and digital DNA-DNA hybridization values	849:920	The average nucleotide identity and digital DNA-DNA hybridization values indicated that strain 1JSPR-7T was a novel species of the genus Lacococcus.
31644419	6	6	theme	rpoB	708:711	arg1	sequence					718:725	the rpoB gene sequence	704:725	the rpoB gene sequence	704:725	hordniae NBRC 100931T (97.1 %), and the rpoB gene sequence showed the highest similarity to L. lactissubsp.
31644419	4	7	theme	L.	606:607	arg1	lactissubsp					609:619	L. lactissubsp	606:619	L. lactissubsp	606:619	The 16S rRNA gene sequence of strain 1JSPR-7T revealed the highest homology with L. lactissubsp.
31644419	9	8	theme	digital	885:891	arg1	hybridization					901:913	digital DNA-DNA hybridization	885:913	digital DNA-DNA hybridization	885:913	The average nucleotide identity and digital DNA-DNA hybridization values indicated that strain 1JSPR-7T was a novel species of the genus Lacococcus.
31644419	11	9	with	diphosphatidylglycerol	1248:1269	arg1	diphosphatidylglycerol					1363:1384	diphosphatidylglycerol	1363:1384	diphosphatidylglycerol	1363:1384	The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid and three unidentified glycolipids with diphosphatidylglycerol as the major one.
31644419	9	10	theme	hybridization	901:913	arg1	values					915:920	The average nucleotide identity and digital DNA-DNA hybridization values	849:920	The average nucleotide identity and digital DNA-DNA hybridization values	849:920	The average nucleotide identity and digital DNA-DNA hybridization values indicated that strain 1JSPR-7T was a novel species of the genus Lacococcus.
31644419	14	11	theme	Lactococcusallomyrinae	1691:1712	arg1	sp					1714:1715	the name Lactococcusallomyrinae sp	1682:1715	the name Lactococcusallomyrinae sp	1682:1715	Based on the data obtained, strain 1JSPR-7T represents a novel species of the genus Lactococcus, for which the name Lactococcusallomyrinae sp.
31644419	12	12	theme	cell-wall	1408:1416	arg1	peptidoglycan					1418:1430	The cell-wall peptidoglycan	1404:1430	The cell-wall peptidoglycan	1404:1430	The cell-wall peptidoglycan was of the A4α type with an interpeptide bridge comprising l-Lys-d-Asp.
31644419	3	13	theme	Comparative	283:293	arg1	analysis					295:302	Comparative analysis	283:302	Comparative analysis of 16S rRNA gene and rpoB gene sequences	283:343	Comparative analysis of 16S rRNA gene and rpoB gene sequences showed that strain 1JSPR-7T fell within the genus Lactococcus, forming a compact cluster with the type strain of four subspecies of Lactococcus lactis and Lactococcus taiwanensis.
31644419	11	14	with	phosphatidylglycerol	1272:1291	arg1	diphosphatidylglycerol					1363:1384	diphosphatidylglycerol	1363:1384	diphosphatidylglycerol	1363:1384	The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid and three unidentified glycolipids with diphosphatidylglycerol as the major one.
31644419	9	15	theme	strain	937:942	arg1	1JSPR-7T					944:951	strain 1JSPR-7T	937:951	strain 1JSPR-7T	937:951	The average nucleotide identity and digital DNA-DNA hybridization values indicated that strain 1JSPR-7T was a novel species of the genus Lacococcus.
31644419	9	15	theme	strain	937:942	arg1	species					965:971	a novel species	957:971	a novel species of the genus Lacococcus	957:995	The average nucleotide identity and digital DNA-DNA hybridization values indicated that strain 1JSPR-7T was a novel species of the genus Lacococcus.
31644419	3	16	theme	taiwanensis	512:522	arg1	subspecies					463:472	four subspecies	458:472	four subspecies of Lactococcus lactis and Lactococcus taiwanensis	458:522	Comparative analysis of 16S rRNA gene and rpoB gene sequences showed that strain 1JSPR-7T fell within the genus Lactococcus, forming a compact cluster with the type strain of four subspecies of Lactococcus lactis and Lactococcus taiwanensis.
31644419	3	17	theme	rRNA	311:314	arg1	gene					316:319	16S rRNA gene	307:319	16S rRNA gene	307:319	Comparative analysis of 16S rRNA gene and rpoB gene sequences showed that strain 1JSPR-7T fell within the genus Lactococcus, forming a compact cluster with the type strain of four subspecies of Lactococcus lactis and Lactococcus taiwanensis.
31644419	14	18	theme	Lactococcus	1659:1669	arg1	species					1638:1644	a novel species	1630:1644	a novel species	1630:1644	Based on the data obtained, strain 1JSPR-7T represents a novel species of the genus Lactococcus, for which the name Lactococcusallomyrinae sp.
31644419	13	19	theme	whole	1537:1541	arg1	sequences					1550:1558	the whole genome sequences	1533:1558	the whole genome sequences	1533:1558	The DNA G+C content based on the whole genome sequences was 37.4 mol%.
31644419	9	20	theme	novel	959:963	arg1	1JSPR-7T					944:951	strain 1JSPR-7T	937:951	strain 1JSPR-7T	937:951	The average nucleotide identity and digital DNA-DNA hybridization values indicated that strain 1JSPR-7T was a novel species of the genus Lacococcus.
31644419	9	20	theme	novel	959:963	arg1	species					965:971	a novel species	957:971	a novel species of the genus Lacococcus	957:995	The average nucleotide identity and digital DNA-DNA hybridization values indicated that strain 1JSPR-7T was a novel species of the genus Lacococcus.
31644419	3	21	theme	gene	330:333	arg1	sequences					335:343	rpoB gene sequences	325:343	rpoB gene sequences	325:343	Comparative analysis of 16S rRNA gene and rpoB gene sequences showed that strain 1JSPR-7T fell within the genus Lactococcus, forming a compact cluster with the type strain of four subspecies of Lactococcus lactis and Lactococcus taiwanensis.
31644419	11	22	with	glycolipids	1346:1356	arg1	diphosphatidylglycerol					1363:1384	diphosphatidylglycerol	1363:1384	diphosphatidylglycerol	1363:1384	The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid and three unidentified glycolipids with diphosphatidylglycerol as the major one.
31644419	13	23	theme	G+C content	1512:1522	arg1	based					1524:1528	The DNA G+C content based	1504:1528	The DNA G+C content based on the whole genome sequences	1504:1558	The DNA G+C content based on the whole genome sequences was 37.4 mol%.
31644419	13	23	theme	G+C content	1512:1522	arg1	%					1572:1572	37.4 mol%	1564:1572	37.4 mol%	1564:1572	The DNA G+C content based on the whole genome sequences was 37.4 mol%.
31644419	9	24	theme	genus	980:984	arg1	Lacococcus					986:995	the genus Lacococcus	976:995	the genus Lacococcus	976:995	The average nucleotide identity and digital DNA-DNA hybridization values indicated that strain 1JSPR-7T was a novel species of the genus Lacococcus.
31644419	10	25	theme	major	1002:1006	arg1	acids					1014:1018	The major fatty acids	998:1018	The major fatty acids (>10 % of the total fatty acids)	998:1051	The major fatty acids (>10 % of the total fatty acids) were summed feature 7 (unknown 18.846, C19 : 1ω6c and/or C19 : 0cyclo ω10c), C16 : 0 and C14 : 0, and the predominant menaquinone was MK-8 with MK-7 as a minor one.
31644419	10	26	dep	C19 	1092:1095	arg1	C19 					1110:1113	C19 	1110:1113	C19 	1110:1113	The major fatty acids (>10 % of the total fatty acids) were summed feature 7 (unknown 18.846, C19 : 1ω6c and/or C19 : 0cyclo ω10c), C16 : 0 and C14 : 0, and the predominant menaquinone was MK-8 with MK-7 as a minor one.
31644419	10	26	dep	C19 	1092:1095	arg1	ω10c					1123:1126	ω10c	1123:1126	ω10c	1123:1126	The major fatty acids (>10 % of the total fatty acids) were summed feature 7 (unknown 18.846, C19 : 1ω6c and/or C19 : 0cyclo ω10c), C16 : 0 and C14 : 0, and the predominant menaquinone was MK-8 with MK-7 as a minor one.
31644419	10	26	dep	C19 	1092:1095	arg1	 1ω6c					1097:1101	 1ω6c	1097:1101	 1ω6c	1097:1101	The major fatty acids (>10 % of the total fatty acids) were summed feature 7 (unknown 18.846, C19 : 1ω6c and/or C19 : 0cyclo ω10c), C16 : 0 and C14 : 0, and the predominant menaquinone was MK-8 with MK-7 as a minor one.
31644419	3	27	theme	strain	357:362	arg1	1JSPR-7T					364:371	strain 1JSPR-7T	357:371	strain 1JSPR-7T	357:371	Comparative analysis of 16S rRNA gene and rpoB gene sequences showed that strain 1JSPR-7T fell within the genus Lactococcus, forming a compact cluster with the type strain of four subspecies of Lactococcus lactis and Lactococcus taiwanensis.
31644419	11	28	with	phospholipid	1310:1321	arg1	diphosphatidylglycerol					1363:1384	diphosphatidylglycerol	1363:1384	diphosphatidylglycerol	1363:1384	The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid and three unidentified glycolipids with diphosphatidylglycerol as the major one.
31644419	11	29	theme	polar	1222:1226	arg1	lipids					1228:1233	The polar lipids	1218:1233	The polar lipids	1218:1233	The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid and three unidentified glycolipids with diphosphatidylglycerol as the major one.
31644419	0	30	theme	Lactococcus	0:10	arg1	sp					24:25	Lactococcus allomyrinae sp	0:25	Lactococcus allomyrinae sp.	0:26	Lactococcus allomyrinae sp.
31644419	1	31	theme	dichotoma	76:84	arg1	larvae					55:60	larvae	55:60	larvae of Allomyrina dichotoma	55:84	nov., isolated from gut of larvae of Allomyrina dichotoma.
31644419	12	32	theme	interpeptide	1460:1471	arg1	bridge					1473:1478	an interpeptide bridge	1457:1478	an interpeptide bridge comprising l-Lys-d-Asp	1457:1501	The cell-wall peptidoglycan was of the A4α type with an interpeptide bridge comprising l-Lys-d-Asp.
31644419	14	33	theme	novel	1632:1636	arg1	species					1638:1644	a novel species	1630:1644	a novel species	1630:1644	Based on the data obtained, strain 1JSPR-7T represents a novel species of the genus Lactococcus, for which the name Lactococcusallomyrinae sp.
31644419	4	34	theme	rRNA	533:536	arg1	sequence					543:550	The 16S rRNA gene sequence	525:550	The 16S rRNA gene sequence of strain 1JSPR-7T	525:569	The 16S rRNA gene sequence of strain 1JSPR-7T revealed the highest homology with L. lactissubsp.
31644419	3	35	theme	compact	418:424	arg1	cluster					426:432	a compact cluster	416:432	a compact cluster	416:432	Comparative analysis of 16S rRNA gene and rpoB gene sequences showed that strain 1JSPR-7T fell within the genus Lactococcus, forming a compact cluster with the type strain of four subspecies of Lactococcus lactis and Lactococcus taiwanensis.
31644419	10	36	theme	acids	1046:1050	arg1	%					1025:1025	>10 %	1021:1025	>10 % of the total fatty acids	1021:1050	The major fatty acids (>10 % of the total fatty acids) were summed feature 7 (unknown 18.846, C19 : 1ω6c and/or C19 : 0cyclo ω10c), C16 : 0 and C14 : 0, and the predominant menaquinone was MK-8 with MK-7 as a minor one.
31644419	10	36	theme	acids	1046:1050	arg1	acids					1046:1050	the total fatty acids	1030:1050	the total fatty acids	1030:1050	The major fatty acids (>10 % of the total fatty acids) were summed feature 7 (unknown 18.846, C19 : 1ω6c and/or C19 : 0cyclo ω10c), C16 : 0 and C14 : 0, and the predominant menaquinone was MK-8 with MK-7 as a minor one.
31644419	16	37	theme	type	1740:1743	arg1	1JSPR-7T					1755:1762	1JSPR-7T	1755:1762	1JSPR-7T (=KACC 19319T=NBRC 113068T)	1755:1790	The type strain is 1JSPR-7T (=KACC 19319T=NBRC 113068T).
31644419	16	37	theme	type	1740:1743	arg1	strain					1745:1750	The type strain	1736:1750	The type strain	1736:1750	The type strain is 1JSPR-7T (=KACC 19319T=NBRC 113068T).
31644419	10	38	theme	total	1034:1038	arg1	acids					1046:1050	the total fatty acids	1030:1050	the total fatty acids	1030:1050	The major fatty acids (>10 % of the total fatty acids) were summed feature 7 (unknown 18.846, C19 : 1ω6c and/or C19 : 0cyclo ω10c), C16 : 0 and C14 : 0, and the predominant menaquinone was MK-8 with MK-7 as a minor one.
31644419	2	39	theme	dichotoma	186:194	arg1	larvae					165:170	larvae	165:170	larvae of Allomyrina dichotoma raised in Wanju-gun, Republic of Korea	165:233	Strain 1JSPR-7T, a facultatively anaerobic bacterium isolated from the gut of larvae of Allomyrina dichotoma raised in Wanju-gun, Republic of Korea, was characterized using a polyphasic approach.
31644419	3	40	theme	type	443:446	arg1	strain					448:453	the type strain	439:453	the type strain of four subspecies of Lactococcus lactis and Lactococcus taiwanensis	439:522	Comparative analysis of 16S rRNA gene and rpoB gene sequences showed that strain 1JSPR-7T fell within the genus Lactococcus, forming a compact cluster with the type strain of four subspecies of Lactococcus lactis and Lactococcus taiwanensis.
31644419	10	41	dep	feature	1065:1071	arg1	C19 					1092:1095	C19 	1092:1095	C19 	1092:1095	The major fatty acids (>10 % of the total fatty acids) were summed feature 7 (unknown 18.846, C19 : 1ω6c and/or C19 : 0cyclo ω10c), C16 : 0 and C14 : 0, and the predominant menaquinone was MK-8 with MK-7 as a minor one.
31644419	3	42	theme	Lactococcus	477:487	arg1	lactis					489:494	Lactococcus lactis	477:494	Lactococcus lactis	477:494	Comparative analysis of 16S rRNA gene and rpoB gene sequences showed that strain 1JSPR-7T fell within the genus Lactococcus, forming a compact cluster with the type strain of four subspecies of Lactococcus lactis and Lactococcus taiwanensis.
31644419	10	43	with	MK-8	1187:1190	arg1	MK-7					1197:1200	MK-7	1197:1200	MK-7	1197:1200	The major fatty acids (>10 % of the total fatty acids) were summed feature 7 (unknown 18.846, C19 : 1ω6c and/or C19 : 0cyclo ω10c), C16 : 0 and C14 : 0, and the predominant menaquinone was MK-8 with MK-7 as a minor one.
31644419	16	44	theme	19319T=NBRC	1771:1781	arg1	1JSPR-7T					1755:1762	1JSPR-7T	1755:1762	1JSPR-7T (=KACC 19319T=NBRC 113068T)	1755:1790	The type strain is 1JSPR-7T (=KACC 19319T=NBRC 113068T).
31644419	16	44	theme	19319T=NBRC	1771:1781	arg1	113068T					1783:1789	=KACC 19319T=NBRC 113068T	1765:1789	=KACC 19319T=NBRC 113068T	1765:1789	The type strain is 1JSPR-7T (=KACC 19319T=NBRC 113068T).
31644419	4	45	theme	highest	584:590	arg1	homology					592:599	the highest homology	580:599	the highest homology with L. lactissubsp	580:619	The 16S rRNA gene sequence of strain 1JSPR-7T revealed the highest homology with L. lactissubsp.
31644419	5	46	theme	lactis	622:627	arg1	5805T					633:637	lactis JCM 5805T	622:637	lactis JCM 5805T (97.3 %)	622:646	lactis JCM 5805T (97.3 %) and L. lactissubsp.
31644419	5	46	theme	lactis	622:627	arg1	%					645:645	97.3 %	640:645	97.3 %	640:645	lactis JCM 5805T (97.3 %) and L. lactissubsp.
31644419	6	47	theme	NBRC	677:680	arg1	100931T					682:688	hordniae NBRC 100931T	668:688	hordniae NBRC 100931T (97.1 %)	668:697	hordniae NBRC 100931T (97.1 %), and the rpoB gene sequence showed the highest similarity to L. lactissubsp.
31644419	6	47	theme	NBRC	677:680	arg1	%					696:696	97.1 %	691:696	97.1 %	691:696	hordniae NBRC 100931T (97.1 %), and the rpoB gene sequence showed the highest similarity to L. lactissubsp.
31644419	3	48	theme	subspecies	463:472	arg1	strain					448:453	the type strain	439:453	the type strain of four subspecies of Lactococcus lactis and Lactococcus taiwanensis	439:522	Comparative analysis of 16S rRNA gene and rpoB gene sequences showed that strain 1JSPR-7T fell within the genus Lactococcus, forming a compact cluster with the type strain of four subspecies of Lactococcus lactis and Lactococcus taiwanensis.
31644419	12	49	with	type	1447:1450	arg1	bridge					1473:1478	an interpeptide bridge	1457:1478	an interpeptide bridge comprising l-Lys-d-Asp	1457:1501	The cell-wall peptidoglycan was of the A4α type with an interpeptide bridge comprising l-Lys-d-Asp.
31644419	6	50	theme	highest	738:744	arg1	similarity					746:755	the highest similarity	734:755	the highest similarity to L. lactissubsp	734:773	hordniae NBRC 100931T (97.1 %), and the rpoB gene sequence showed the highest similarity to L. lactissubsp.
31644419	2	51	theme	polyphasic	262:271	arg1	approach					273:280	a polyphasic approach	260:280	a polyphasic approach	260:280	Strain 1JSPR-7T, a facultatively anaerobic bacterium isolated from the gut of larvae of Allomyrina dichotoma raised in Wanju-gun, Republic of Korea, was characterized using a polyphasic approach.
31644419	4	52	theme	strain	555:560	arg1	1JSPR-7T					562:569	strain 1JSPR-7T	555:569	strain 1JSPR-7T	555:569	The 16S rRNA gene sequence of strain 1JSPR-7T revealed the highest homology with L. lactissubsp.
31644419	14	53	theme	strain	1603:1608	arg1	1JSPR-7T					1610:1617	strain 1JSPR-7T	1603:1617	strain 1JSPR-7T	1603:1617	Based on the data obtained, strain 1JSPR-7T represents a novel species of the genus Lactococcus, for which the name Lactococcusallomyrinae sp.
31644419	9	54	theme	nucleotide	861:870	arg1	identity					872:879	average nucleotide identity	853:879	average nucleotide identity	853:879	The average nucleotide identity and digital DNA-DNA hybridization values indicated that strain 1JSPR-7T was a novel species of the genus Lacococcus.
31644419	2	55	theme	anaerobic	120:128	arg1	bacterium					130:138	a facultatively anaerobic bacterium	104:138	a facultatively anaerobic bacterium isolated from the gut of larvae of Allomyrina dichotoma raised in Wanju-gun, Republic of Korea	104:233	Strain 1JSPR-7T, a facultatively anaerobic bacterium isolated from the gut of larvae of Allomyrina dichotoma raised in Wanju-gun, Republic of Korea, was characterized using a polyphasic approach.
31644419	2	55	theme	anaerobic	120:128	arg1	Strain					87:92	Strain 1JSPR-7T	87:101	Strain 1JSPR-7T	87:101	Strain 1JSPR-7T, a facultatively anaerobic bacterium isolated from the gut of larvae of Allomyrina dichotoma raised in Wanju-gun, Republic of Korea, was characterized using a polyphasic approach.
31644419	6	56	theme	gene	713:716	arg1	sequence					718:725	the rpoB gene sequence	704:725	the rpoB gene sequence	704:725	hordniae NBRC 100931T (97.1 %), and the rpoB gene sequence showed the highest similarity to L. lactissubsp.
31644419	7	57	theme	DSM	785:787	arg1	20069T					789:794	cremoris DSM 20069T	776:794	cremoris DSM 20069T (91.4 %)	776:803	cremoris DSM 20069T (91.4 %) and L. lactissubsp.
31644419	7	57	theme	DSM	785:787	arg1	%					802:802	91.4 %	797:802	91.4 %	797:802	cremoris DSM 20069T (91.4 %) and L. lactissubsp.
31644419	12	58	theme	A4α	1443:1445	arg1	type					1447:1450	the A4α type	1439:1450	the A4α type	1439:1450	The cell-wall peptidoglycan was of the A4α type with an interpeptide bridge comprising l-Lys-d-Asp.
31644419	10	59	theme	minor	1207:1211	arg1	one					1213:1215	a minor one	1205:1215	a minor one	1205:1215	The major fatty acids (>10 % of the total fatty acids) were summed feature 7 (unknown 18.846, C19 : 1ω6c and/or C19 : 0cyclo ω10c), C16 : 0 and C14 : 0, and the predominant menaquinone was MK-8 with MK-7 as a minor one.
31644419	5	60	theme	L.	652:653	arg1	lactissubsp					655:665	L. lactissubsp	652:665	L. lactissubsp	652:665	lactis JCM 5805T (97.3 %) and L. lactissubsp.
31644419	10	61	dep	acids	1014:1018	arg1	%					1025:1025	>10 %	1021:1025	>10 % of the total fatty acids	1021:1050	The major fatty acids (>10 % of the total fatty acids) were summed feature 7 (unknown 18.846, C19 : 1ω6c and/or C19 : 0cyclo ω10c), C16 : 0 and C14 : 0, and the predominant menaquinone was MK-8 with MK-7 as a minor one.
31644419	10	61	dep	acids	1014:1018	arg1	acids					1046:1050	the total fatty acids	1030:1050	the total fatty acids	1030:1050	The major fatty acids (>10 % of the total fatty acids) were summed feature 7 (unknown 18.846, C19 : 1ω6c and/or C19 : 0cyclo ω10c), C16 : 0 and C14 : 0, and the predominant menaquinone was MK-8 with MK-7 as a minor one.
31644419	13	62	theme	37.4 mol	1564:1571	arg1	%					1572:1572	37.4 mol%	1564:1572	37.4 mol%	1564:1572	The DNA G+C content based on the whole genome sequences was 37.4 mol%.
31644419	13	62	theme	37.4 mol	1564:1571	arg1	based					1524:1528	The DNA G+C content based	1504:1528	The DNA G+C content based on the whole genome sequences	1504:1558	The DNA G+C content based on the whole genome sequences was 37.4 mol%.
31644419	4	63	with	homology	592:599	arg1	lactissubsp					609:619	L. lactissubsp	606:619	L. lactissubsp	606:619	The 16S rRNA gene sequence of strain 1JSPR-7T revealed the highest homology with L. lactissubsp.
31644419	11	64	theme	major	1393:1397	arg1	one					1399:1401	the major one	1389:1401	the major one	1389:1401	The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid and three unidentified glycolipids with diphosphatidylglycerol as the major one.
31644419	14	65	theme	name	1686:1689	arg1	sp					1714:1715	the name Lactococcusallomyrinae sp	1682:1715	the name Lactococcusallomyrinae sp	1682:1715	Based on the data obtained, strain 1JSPR-7T represents a novel species of the genus Lactococcus, for which the name Lactococcusallomyrinae sp.
31644419	11	66	theme	unidentified	1297:1308	arg1	phospholipid					1310:1321	an unidentified phospholipid	1294:1321	an unidentified phospholipid	1294:1321	The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid and three unidentified glycolipids with diphosphatidylglycerol as the major one.
31644419	6	67	theme	L.	760:761	arg1	lactissubsp					763:773	L. lactissubsp	760:773	L. lactissubsp	760:773	hordniae NBRC 100931T (97.1 %), and the rpoB gene sequence showed the highest similarity to L. lactissubsp.
31644419	3	68	theme	gene	316:319	arg1	analysis					295:302	Comparative analysis	283:302	Comparative analysis of 16S rRNA gene and rpoB gene sequences	283:343	Comparative analysis of 16S rRNA gene and rpoB gene sequences showed that strain 1JSPR-7T fell within the genus Lactococcus, forming a compact cluster with the type strain of four subspecies of Lactococcus lactis and Lactococcus taiwanensis.
31644419	11	69	theme	unidentified	1333:1344	arg1	glycolipids					1346:1356	three unidentified glycolipids	1327:1356	three unidentified glycolipids with diphosphatidylglycerol as the major one	1327:1401	The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid and three unidentified glycolipids with diphosphatidylglycerol as the major one.
31644419	14	70	theme	genus	1653:1657	arg1	Lactococcus					1659:1669	the genus Lactococcus	1649:1669	the genus Lactococcus	1649:1669	Based on the data obtained, strain 1JSPR-7T represents a novel species of the genus Lactococcus, for which the name Lactococcusallomyrinae sp.
31644419	3	71	theme	16S	307:309	arg1	rRNA					311:314	16S rRNA	307:314	16S rRNA gene	307:319	Comparative analysis of 16S rRNA gene and rpoB gene sequences showed that strain 1JSPR-7T fell within the genus Lactococcus, forming a compact cluster with the type strain of four subspecies of Lactococcus lactis and Lactococcus taiwanensis.
31644419	13	72	theme	genome	1543:1548	arg1	sequences					1550:1558	the whole genome sequences	1533:1558	the whole genome sequences	1533:1558	The DNA G+C content based on the whole genome sequences was 37.4 mol%.
31644419	3	73	theme	sequences	335:343	arg1	analysis					295:302	Comparative analysis	283:302	Comparative analysis of 16S rRNA gene and rpoB gene sequences	283:343	Comparative analysis of 16S rRNA gene and rpoB gene sequences showed that strain 1JSPR-7T fell within the genus Lactococcus, forming a compact cluster with the type strain of four subspecies of Lactococcus lactis and Lactococcus taiwanensis.
31644419	6	74	theme	hordniae	668:675	arg1	100931T					682:688	hordniae NBRC 100931T	668:688	hordniae NBRC 100931T (97.1 %)	668:697	hordniae NBRC 100931T (97.1 %), and the rpoB gene sequence showed the highest similarity to L. lactissubsp.
31644419	6	74	theme	hordniae	668:675	arg1	%					696:696	97.1 %	691:696	97.1 %	691:696	hordniae NBRC 100931T (97.1 %), and the rpoB gene sequence showed the highest similarity to L. lactissubsp.
31644419	10	75	theme	fatty	1008:1012	arg1	acids					1014:1018	The major fatty acids	998:1018	The major fatty acids (>10 % of the total fatty acids)	998:1051	The major fatty acids (>10 % of the total fatty acids) were summed feature 7 (unknown 18.846, C19 : 1ω6c and/or C19 : 0cyclo ω10c), C16 : 0 and C14 : 0, and the predominant menaquinone was MK-8 with MK-7 as a minor one.
31644419	1	76	theme	larvae	55:60	arg1	gut					48:50	gut	48:50	gut of larvae of Allomyrina dichotoma	48:84	nov., isolated from gut of larvae of Allomyrina dichotoma.
31644419	3	77	theme	rpoB	325:328	arg1	sequences					335:343	rpoB gene sequences	325:343	rpoB gene sequences	325:343	Comparative analysis of 16S rRNA gene and rpoB gene sequences showed that strain 1JSPR-7T fell within the genus Lactococcus, forming a compact cluster with the type strain of four subspecies of Lactococcus lactis and Lactococcus taiwanensis.
31644419	9	78	theme	DNA-DNA	893:899	arg1	hybridization					901:913	digital DNA-DNA hybridization	885:913	digital DNA-DNA hybridization	885:913	The average nucleotide identity and digital DNA-DNA hybridization values indicated that strain 1JSPR-7T was a novel species of the genus Lacococcus.
31644419	9	79	theme	Lacococcus	986:995	arg1	1JSPR-7T					944:951	strain 1JSPR-7T	937:951	strain 1JSPR-7T	937:951	The average nucleotide identity and digital DNA-DNA hybridization values indicated that strain 1JSPR-7T was a novel species of the genus Lacococcus.
31644419	9	79	theme	Lacococcus	986:995	arg1	species					965:971	a novel species	957:971	a novel species of the genus Lacococcus	957:995	The average nucleotide identity and digital DNA-DNA hybridization values indicated that strain 1JSPR-7T was a novel species of the genus Lacococcus.
31644419	0	80	theme	allomyrinae	12:22	arg1	sp					24:25	Lactococcus allomyrinae sp	0:25	Lactococcus allomyrinae sp.	0:26	Lactococcus allomyrinae sp.
31644419	1	81	theme	Allomyrina	65:74	arg1	dichotoma					76:84	Allomyrina dichotoma	65:84	Allomyrina dichotoma	65:84	nov., isolated from gut of larvae of Allomyrina dichotoma.
31644419	4	82	theme	16S	529:531	arg1	rRNA					533:536	16S rRNA	529:536	The 16S rRNA gene sequence of strain 1JSPR-7T	525:569	The 16S rRNA gene sequence of strain 1JSPR-7T revealed the highest homology with L. lactissubsp.
31644419	4	83	theme	gene	538:541	arg1	sequence					543:550	The 16S rRNA gene sequence	525:550	The 16S rRNA gene sequence of strain 1JSPR-7T	525:569	The 16S rRNA gene sequence of strain 1JSPR-7T revealed the highest homology with L. lactissubsp.
31644419	2	84	theme	larvae	165:170	arg1	gut					158:160	the gut	154:160	the gut of larvae of Allomyrina dichotoma raised in Wanju-gun, Republic of Korea	154:233	Strain 1JSPR-7T, a facultatively anaerobic bacterium isolated from the gut of larvae of Allomyrina dichotoma raised in Wanju-gun, Republic of Korea, was characterized using a polyphasic approach.
31644419	13	85	from	based	1524:1528	arg1	sequences					1550:1558	the whole genome sequences	1533:1558	the whole genome sequences	1533:1558	The DNA G+C content based on the whole genome sequences was 37.4 mol%.
31644419	3	86	theme	genus	389:393	arg1	Lactococcus					395:405	the genus Lactococcus	385:405	the genus Lactococcus	385:405	Comparative analysis of 16S rRNA gene and rpoB gene sequences showed that strain 1JSPR-7T fell within the genus Lactococcus, forming a compact cluster with the type strain of four subspecies of Lactococcus lactis and Lactococcus taiwanensis.
31644419	13	87	theme	DNA	1508:1510	arg1	based					1524:1528	The DNA G+C content based	1504:1528	The DNA G+C content based on the whole genome sequences	1504:1558	The DNA G+C content based on the whole genome sequences was 37.4 mol%.
31644419	13	87	theme	DNA	1508:1510	arg1	%					1572:1572	37.4 mol%	1564:1572	37.4 mol%	1564:1572	The DNA G+C content based on the whole genome sequences was 37.4 mol%.
31644419	10	88	theme	fatty	1040:1044	arg1	acids					1046:1050	the total fatty acids	1030:1050	the total fatty acids	1030:1050	The major fatty acids (>10 % of the total fatty acids) were summed feature 7 (unknown 18.846, C19 : 1ω6c and/or C19 : 0cyclo ω10c), C16 : 0 and C14 : 0, and the predominant menaquinone was MK-8 with MK-7 as a minor one.
31644419	2	89	attach	isolated	140:147	arg2	Strain					87:92	Strain 1JSPR-7T	87:101	Strain 1JSPR-7T	87:101	Strain 1JSPR-7T, a facultatively anaerobic bacterium isolated from the gut of larvae of Allomyrina dichotoma raised in Wanju-gun, Republic of Korea, was characterized using a polyphasic approach.
31644419	2	89	attach	isolated	140:147	arg2	bacterium					130:138	a facultatively anaerobic bacterium	104:138	a facultatively anaerobic bacterium isolated from the gut of larvae of Allomyrina dichotoma raised in Wanju-gun, Republic of Korea	104:233	Strain 1JSPR-7T, a facultatively anaerobic bacterium isolated from the gut of larvae of Allomyrina dichotoma raised in Wanju-gun, Republic of Korea, was characterized using a polyphasic approach.
31644419	2	89	attach	isolated	140:147	arg1	gut					158:160	the gut	154:160	the gut of larvae of Allomyrina dichotoma raised in Wanju-gun, Republic of Korea	154:233	Strain 1JSPR-7T, a facultatively anaerobic bacterium isolated from the gut of larvae of Allomyrina dichotoma raised in Wanju-gun, Republic of Korea, was characterized using a polyphasic approach.
31644419	16	90	theme	=KACC	1765:1769	arg1	1JSPR-7T					1755:1762	1JSPR-7T	1755:1762	1JSPR-7T (=KACC 19319T=NBRC 113068T)	1755:1790	The type strain is 1JSPR-7T (=KACC 19319T=NBRC 113068T).
31644419	16	90	theme	=KACC	1765:1769	arg1	113068T					1783:1789	=KACC 19319T=NBRC 113068T	1765:1789	=KACC 19319T=NBRC 113068T	1765:1789	The type strain is 1JSPR-7T (=KACC 19319T=NBRC 113068T).
31644419	2	91	theme	Allomyrina	175:184	arg1	dichotoma					186:194	Allomyrina dichotoma	175:194	Allomyrina dichotoma raised in Wanju-gun, Republic of Korea	175:233	Strain 1JSPR-7T, a facultatively anaerobic bacterium isolated from the gut of larvae of Allomyrina dichotoma raised in Wanju-gun, Republic of Korea, was characterized using a polyphasic approach.
31644419	7	92	theme	L.	809:810	arg1	lactissubsp					812:822	L. lactissubsp	809:822	L. lactissubsp	809:822	cremoris DSM 20069T (91.4 %) and L. lactissubsp.
31644419	10	93	theme	predominant	1159:1169	arg1	MK-8					1187:1190	MK-8	1187:1190	MK-8 with MK-7	1187:1200	The major fatty acids (>10 % of the total fatty acids) were summed feature 7 (unknown 18.846, C19 : 1ω6c and/or C19 : 0cyclo ω10c), C16 : 0 and C14 : 0, and the predominant menaquinone was MK-8 with MK-7 as a minor one.
31644419	10	93	theme	predominant	1159:1169	arg1	menaquinone					1171:1181	the predominant menaquinone	1155:1181	the predominant menaquinone	1155:1181	The major fatty acids (>10 % of the total fatty acids) were summed feature 7 (unknown 18.846, C19 : 1ω6c and/or C19 : 0cyclo ω10c), C16 : 0 and C14 : 0, and the predominant menaquinone was MK-8 with MK-7 as a minor one.
31644419	3	94	theme	lactis	489:494	arg1	subspecies					463:472	four subspecies	458:472	four subspecies of Lactococcus lactis and Lactococcus taiwanensis	458:522	Comparative analysis of 16S rRNA gene and rpoB gene sequences showed that strain 1JSPR-7T fell within the genus Lactococcus, forming a compact cluster with the type strain of four subspecies of Lactococcus lactis and Lactococcus taiwanensis.
29557772	2	0	theme	heterotrophic	99:111	arg1	bacterium					174:182	A novel heterotrophic, Gram-stain-positive, facultatively anaerobic and rod-shaped bacterium	91:182	A novel heterotrophic, Gram-stain-positive, facultatively anaerobic and rod-shaped bacterium	91:182	A novel heterotrophic, Gram-stain-positive, facultatively anaerobic and rod-shaped bacterium, designated strain NBT06-6T, was isolated from the deep seawater in the New Britain Trench and characterized phylogenetically and phenotypically.
29557772	7	1	theme	major	1034:1038	arg1	acids					1046:1050	The major fatty acids	1030:1050	The major fatty acids detected	1030:1059	The major fatty acids detected were C18 : 1ω9c, C16 : 0 and C15 : 0.
29557772	7	1	theme	major	1034:1038	arg1	C18 					1066:1069	C18 	1066:1069	C18 	1066:1069	The major fatty acids detected were C18 : 1ω9c, C16 : 0 and C15 : 0.
29557772	4	2	theme	gene	745:748	arg1	similarities					759:770	low 16S rRNA gene sequence similarities	732:770	low 16S rRNA gene sequence similarities	732:770	The strain grew at hydrostatic pressures of 0.1-100 MPa (optimum, 0.1 MPa) at 35 °C. Phylogenetic analysis of the 16S rRNA gene sequences indicated that strain NBT06-6T belonged to the genus Corynebacterium, with the highest sequence similarity (97.9 %) to Corynebacterium imitans, and shared low 16S rRNA gene sequence similarities (<97.0 %) with other type strains.
29557772	2	3	theme	Britain	260:266	arg1	Trench					268:273	the New Britain Trench	252:273	the New Britain Trench	252:273	A novel heterotrophic, Gram-stain-positive, facultatively anaerobic and rod-shaped bacterium, designated strain NBT06-6T, was isolated from the deep seawater in the New Britain Trench and characterized phylogenetically and phenotypically.
29557772	9	4	theme	DNA	1299:1301	arg1	G+C content					1272:1282	The G+C content	1268:1282	The G+C content of the genomic DNA	1268:1301	The G+C content of the genomic DNA was 65.1 mol%.
29557772	9	4	theme	DNA	1299:1301	arg1	%					1315:1315	65.1 mol%	1307:1315	65.1 mol%	1307:1315	The G+C content of the genomic DNA was 65.1 mol%.
29557772	8	5	theme	meso-diaminopimelic	1125:1143	arg1	acid					1145:1148	meso-diaminopimelic acid	1125:1148	meso-diaminopimelic acid	1125:1148	Strain NBT06-6T contained meso-diaminopimelic acid and mycolic acids in its cell wall, and mannose, galactose, glucose, arabinose and ribose as major whole-cell sugars.
29557772	9	6	theme	65.1 mol	1307:1314	arg1	G+C content					1272:1282	The G+C content	1268:1282	The G+C content of the genomic DNA	1268:1301	The G+C content of the genomic DNA was 65.1 mol%.
29557772	9	6	theme	65.1 mol	1307:1314	arg1	%					1315:1315	65.1 mol%	1307:1315	65.1 mol%	1307:1315	The G+C content of the genomic DNA was 65.1 mol%.
29557772	4	7	theme	16S	553:555	arg1	sequences					567:575	the 16S rRNA gene sequences	549:575	the 16S rRNA gene sequences	549:575	The strain grew at hydrostatic pressures of 0.1-100 MPa (optimum, 0.1 MPa) at 35 °C. Phylogenetic analysis of the 16S rRNA gene sequences indicated that strain NBT06-6T belonged to the genus Corynebacterium, with the highest sequence similarity (97.9 %) to Corynebacterium imitans, and shared low 16S rRNA gene sequence similarities (<97.0 %) with other type strains.
29557772	4	8	theme	gene	562:565	arg1	sequences					567:575	the 16S rRNA gene sequences	549:575	the 16S rRNA gene sequences	549:575	The strain grew at hydrostatic pressures of 0.1-100 MPa (optimum, 0.1 MPa) at 35 °C. Phylogenetic analysis of the 16S rRNA gene sequences indicated that strain NBT06-6T belonged to the genus Corynebacterium, with the highest sequence similarity (97.9 %) to Corynebacterium imitans, and shared low 16S rRNA gene sequence similarities (<97.0 %) with other type strains.
29557772	8	9	theme	Strain	1099:1104	arg1	NBT06-6T					1106:1113	Strain NBT06-6T	1099:1113	Strain NBT06-6T	1099:1113	Strain NBT06-6T contained meso-diaminopimelic acid and mycolic acids in its cell wall, and mannose, galactose, glucose, arabinose and ribose as major whole-cell sugars.
29557772	4	10	theme	other	787:791	arg1	strains					798:804	other type strains	787:804	other type strains	787:804	The strain grew at hydrostatic pressures of 0.1-100 MPa (optimum, 0.1 MPa) at 35 °C. Phylogenetic analysis of the 16S rRNA gene sequences indicated that strain NBT06-6T belonged to the genus Corynebacterium, with the highest sequence similarity (97.9 %) to Corynebacterium imitans, and shared low 16S rRNA gene sequence similarities (<97.0 %) with other type strains.
29557772	11	11	theme	phenotypic	1500:1509	arg1	data					1511:1514	The combined genotypic and phenotypic data	1473:1514	The combined genotypic and phenotypic data	1473:1514	The combined genotypic and phenotypic data indicated that strain NBT06-6T represents a novel species of the genus Corynebacterium, for which the name Corynebacterium hadale sp.
29557772	8	12	contain	contained	1115:1123	arg2	acids					1162:1166	mycolic acids	1154:1166	mycolic acids	1154:1166	Strain NBT06-6T contained meso-diaminopimelic acid and mycolic acids in its cell wall, and mannose, galactose, glucose, arabinose and ribose as major whole-cell sugars.
29557772	8	12	contain	contained	1115:1123	arg2	mannose					1190:1196	mannose	1190:1196	mannose	1190:1196	Strain NBT06-6T contained meso-diaminopimelic acid and mycolic acids in its cell wall, and mannose, galactose, glucose, arabinose and ribose as major whole-cell sugars.
29557772	8	12	contain	contained	1115:1123	arg1	NBT06-6T					1106:1113	Strain NBT06-6T	1099:1113	Strain NBT06-6T	1099:1113	Strain NBT06-6T contained meso-diaminopimelic acid and mycolic acids in its cell wall, and mannose, galactose, glucose, arabinose and ribose as major whole-cell sugars.
29557772	8	12	contain	contained	1115:1123	arg2	acid					1145:1148	meso-diaminopimelic acid	1125:1148	meso-diaminopimelic acid	1125:1148	Strain NBT06-6T contained meso-diaminopimelic acid and mycolic acids in its cell wall, and mannose, galactose, glucose, arabinose and ribose as major whole-cell sugars.
29557772	8	13	from	acids	1162:1166	arg1	wall					1180:1183	its cell wall	1171:1183	its cell wall	1171:1183	Strain NBT06-6T contained meso-diaminopimelic acid and mycolic acids in its cell wall, and mannose, galactose, glucose, arabinose and ribose as major whole-cell sugars.
29557772	5	14	theme	respiratory	817:827	arg1	MK-8					847:850	MK-8	847:850	MK-8	847:850	The major respiratory menaquinones were MK-8(H2) and MK-9(H2).
29557772	5	14	theme	respiratory	817:827	arg1	menaquinones					829:840	The major respiratory menaquinones	807:840	The major respiratory menaquinones	807:840	The major respiratory menaquinones were MK-8(H2) and MK-9(H2).
29557772	3	15	theme	Optimal	330:336	arg1	growth					348:353	Optimal bacterial growth	330:353	Optimal bacterial growth	330:353	Optimal bacterial growth occurred at 35 °C (range 22-41 °C), at pH 6 (4-8) and with 4 % (w/v) NaCl (0-10 %).
29557772	7	16	dep	C18 	1066:1069	arg1	 0					1083:1084	 0	1083:1084	 0	1083:1084	The major fatty acids detected were C18 : 1ω9c, C16 : 0 and C15 : 0.
29557772	7	16	dep	C18 	1066:1069	arg1	C16 					1078:1081	C16 	1078:1081	C16 	1078:1081	The major fatty acids detected were C18 : 1ω9c, C16 : 0 and C15 : 0.
29557772	7	16	dep	C18 	1066:1069	arg1	C15 					1090:1093	C15 	1090:1093	C15 	1090:1093	The major fatty acids detected were C18 : 1ω9c, C16 : 0 and C15 : 0.
29557772	11	17	theme	Corynebacterium	1623:1637	arg1	sp					1646:1647	the name Corynebacterium hadale sp	1614:1647	the name Corynebacterium hadale sp	1614:1647	The combined genotypic and phenotypic data indicated that strain NBT06-6T represents a novel species of the genus Corynebacterium, for which the name Corynebacterium hadale sp.
29557772	4	18	dep	optimum	496:502	arg1	0.1 MPa					505:511	0.1 MPa	505:511	0.1 MPa	505:511	The strain grew at hydrostatic pressures of 0.1-100 MPa (optimum, 0.1 MPa) at 35 °C. Phylogenetic analysis of the 16S rRNA gene sequences indicated that strain NBT06-6T belonged to the genus Corynebacterium, with the highest sequence similarity (97.9 %) to Corynebacterium imitans, and shared low 16S rRNA gene sequence similarities (<97.0 %) with other type strains.
29557772	10	19	theme	digital	1322:1328	arg1	hybridization					1338:1350	The digital DNA-DNA hybridization	1318:1350	The digital DNA-DNA hybridization value	1318:1356	The digital DNA-DNA hybridization value and the average nucleotide identity between strain NBT06-6T and C. imitans were 24.5±2.4 and 81.9 %, respectively.
29557772	1	20	theme	New	71:73	arg1	Trench					83:88	the New Britain Trench	67:88	the New Britain Trench	67:88	nov. isolated from hadopelagic water of the New Britain Trench.
29557772	4	21	theme	highest	656:662	arg1	similarity					673:682	the highest sequence similarity	652:682	the highest sequence similarity (97.9 %) to Corynebacterium imitans	652:718	The strain grew at hydrostatic pressures of 0.1-100 MPa (optimum, 0.1 MPa) at 35 °C. Phylogenetic analysis of the 16S rRNA gene sequences indicated that strain NBT06-6T belonged to the genus Corynebacterium, with the highest sequence similarity (97.9 %) to Corynebacterium imitans, and shared low 16S rRNA gene sequence similarities (<97.0 %) with other type strains.
29557772	4	21	theme	highest	656:662	arg1	%					690:690	97.9 %	685:690	97.9 %	685:690	The strain grew at hydrostatic pressures of 0.1-100 MPa (optimum, 0.1 MPa) at 35 °C. Phylogenetic analysis of the 16S rRNA gene sequences indicated that strain NBT06-6T belonged to the genus Corynebacterium, with the highest sequence similarity (97.9 %) to Corynebacterium imitans, and shared low 16S rRNA gene sequence similarities (<97.0 %) with other type strains.
29557772	1	22	theme	Trench	83:88	arg1	water					58:62	hadopelagic water	46:62	hadopelagic water of the New Britain Trench	46:88	nov. isolated from hadopelagic water of the New Britain Trench.
29557772	3	23	theme	w/v	419:421	arg1	NaCl					424:427	4 % (w/v) NaCl	414:427	4 % (w/v) NaCl (0-10 %)	414:436	Optimal bacterial growth occurred at 35 °C (range 22-41 °C), at pH 6 (4-8) and with 4 % (w/v) NaCl (0-10 %).
29557772	3	23	theme	w/v	419:421	arg1	%					435:435	0-10 %	430:435	0-10 %	430:435	Optimal bacterial growth occurred at 35 °C (range 22-41 °C), at pH 6 (4-8) and with 4 % (w/v) NaCl (0-10 %).
29557772	10	24	theme	hybridization	1338:1350	arg1	value					1352:1356	The digital DNA-DNA hybridization value	1318:1356	The digital DNA-DNA hybridization value	1318:1356	The digital DNA-DNA hybridization value and the average nucleotide identity between strain NBT06-6T and C. imitans were 24.5±2.4 and 81.9 %, respectively.
29557772	8	25	theme	whole-cell	1249:1258	arg1	sugars					1260:1265	major whole-cell sugars	1243:1265	major whole-cell sugars	1243:1265	Strain NBT06-6T contained meso-diaminopimelic acid and mycolic acids in its cell wall, and mannose, galactose, glucose, arabinose and ribose as major whole-cell sugars.
29557772	3	26	theme	%	416:416	arg1	NaCl					424:427	4 % (w/v) NaCl	414:427	4 % (w/v) NaCl (0-10 %)	414:436	Optimal bacterial growth occurred at 35 °C (range 22-41 °C), at pH 6 (4-8) and with 4 % (w/v) NaCl (0-10 %).
29557772	3	26	theme	%	416:416	arg1	%					435:435	0-10 %	430:435	0-10 %	430:435	Optimal bacterial growth occurred at 35 °C (range 22-41 °C), at pH 6 (4-8) and with 4 % (w/v) NaCl (0-10 %).
29557772	2	27	theme	rod-shaped	163:172	arg1	bacterium					174:182	A novel heterotrophic, Gram-stain-positive, facultatively anaerobic and rod-shaped bacterium	91:182	A novel heterotrophic, Gram-stain-positive, facultatively anaerobic and rod-shaped bacterium	91:182	A novel heterotrophic, Gram-stain-positive, facultatively anaerobic and rod-shaped bacterium, designated strain NBT06-6T, was isolated from the deep seawater in the New Britain Trench and characterized phylogenetically and phenotypically.
29557772	4	28	theme	16S	736:738	arg1	similarities					759:770	low 16S rRNA gene sequence similarities	732:770	low 16S rRNA gene sequence similarities	732:770	The strain grew at hydrostatic pressures of 0.1-100 MPa (optimum, 0.1 MPa) at 35 °C. Phylogenetic analysis of the 16S rRNA gene sequences indicated that strain NBT06-6T belonged to the genus Corynebacterium, with the highest sequence similarity (97.9 %) to Corynebacterium imitans, and shared low 16S rRNA gene sequence similarities (<97.0 %) with other type strains.
29557772	12	29	theme	strain	1682:1687	arg1	NBT06-6T					1689:1696	the type strain NBT06-6T	1673:1696	the type strain NBT06-6T (=MCCC 1K03347T=DSM 105365T)	1673:1725	nov. is proposed, with the type strain NBT06-6T (=MCCC 1K03347T=DSM 105365T).
29557772	12	29	theme	strain	1682:1687	arg1	105365T					1718:1724	=MCCC 1K03347T=DSM 105365T	1699:1724	=MCCC 1K03347T=DSM 105365T	1699:1724	nov. is proposed, with the type strain NBT06-6T (=MCCC 1K03347T=DSM 105365T).
29557772	4	30	theme	hydrostatic	458:468	arg1	pressures					470:478	hydrostatic pressures	458:478	hydrostatic pressures of 0.1-100 MPa (optimum, 0.1 MPa)	458:512	The strain grew at hydrostatic pressures of 0.1-100 MPa (optimum, 0.1 MPa) at 35 °C. Phylogenetic analysis of the 16S rRNA gene sequences indicated that strain NBT06-6T belonged to the genus Corynebacterium, with the highest sequence similarity (97.9 %) to Corynebacterium imitans, and shared low 16S rRNA gene sequence similarities (<97.0 %) with other type strains.
29557772	10	31	theme	average	1366:1372	arg1	%					1456:1456	24.5±2.4 and 81.9 %	1438:1456	24.5±2.4 and 81.9 %	1438:1456	The digital DNA-DNA hybridization value and the average nucleotide identity between strain NBT06-6T and C. imitans were 24.5±2.4 and 81.9 %, respectively.
29557772	10	31	theme	average	1366:1372	arg1	identity					1385:1392	the average nucleotide identity	1362:1392	the average nucleotide identity between strain NBT06-6T and C. imitans	1362:1431	The digital DNA-DNA hybridization value and the average nucleotide identity between strain NBT06-6T and C. imitans were 24.5±2.4 and 81.9 %, respectively.
29557772	8	32	from	glucose	1210:1216	arg1	wall					1180:1183	its cell wall	1171:1183	its cell wall	1171:1183	Strain NBT06-6T contained meso-diaminopimelic acid and mycolic acids in its cell wall, and mannose, galactose, glucose, arabinose and ribose as major whole-cell sugars.
29557772	12	33	theme	=MCCC	1699:1703	arg1	NBT06-6T					1689:1696	the type strain NBT06-6T	1673:1696	the type strain NBT06-6T (=MCCC 1K03347T=DSM 105365T)	1673:1725	nov. is proposed, with the type strain NBT06-6T (=MCCC 1K03347T=DSM 105365T).
29557772	12	33	theme	=MCCC	1699:1703	arg1	105365T					1718:1724	=MCCC 1K03347T=DSM 105365T	1699:1724	=MCCC 1K03347T=DSM 105365T	1699:1724	nov. is proposed, with the type strain NBT06-6T (=MCCC 1K03347T=DSM 105365T).
29557772	11	34	theme	genus	1581:1585	arg1	Corynebacterium					1587:1601	the genus Corynebacterium	1577:1601	the genus Corynebacterium	1577:1601	The combined genotypic and phenotypic data indicated that strain NBT06-6T represents a novel species of the genus Corynebacterium, for which the name Corynebacterium hadale sp.
29557772	11	35	theme	genotypic	1486:1494	arg1	data					1511:1514	The combined genotypic and phenotypic data	1473:1514	The combined genotypic and phenotypic data	1473:1514	The combined genotypic and phenotypic data indicated that strain NBT06-6T represents a novel species of the genus Corynebacterium, for which the name Corynebacterium hadale sp.
29557772	8	36	from	galactose	1199:1207	arg1	wall					1180:1183	its cell wall	1171:1183	its cell wall	1171:1183	Strain NBT06-6T contained meso-diaminopimelic acid and mycolic acids in its cell wall, and mannose, galactose, glucose, arabinose and ribose as major whole-cell sugars.
29557772	1	37	theme	hadopelagic	46:56	arg1	water					58:62	hadopelagic water	46:62	hadopelagic water of the New Britain Trench	46:88	nov. isolated from hadopelagic water of the New Britain Trench.
29557772	10	38	theme	strain	1402:1407	arg1	NBT06-6T					1409:1416	strain NBT06-6T	1402:1416	strain NBT06-6T	1402:1416	The digital DNA-DNA hybridization value and the average nucleotide identity between strain NBT06-6T and C. imitans were 24.5±2.4 and 81.9 %, respectively.
29557772	8	39	from	ribose	1233:1238	arg1	wall					1180:1183	its cell wall	1171:1183	its cell wall	1171:1183	Strain NBT06-6T contained meso-diaminopimelic acid and mycolic acids in its cell wall, and mannose, galactose, glucose, arabinose and ribose as major whole-cell sugars.
29557772	11	40	theme	novel	1560:1564	arg1	species					1566:1572	a novel species	1558:1572	a novel species	1558:1572	The combined genotypic and phenotypic data indicated that strain NBT06-6T represents a novel species of the genus Corynebacterium, for which the name Corynebacterium hadale sp.
29557772	10	41	theme	24.5±2.4 and	1438:1449	arg1	%					1456:1456	24.5±2.4 and 81.9 %	1438:1456	24.5±2.4 and 81.9 %	1438:1456	The digital DNA-DNA hybridization value and the average nucleotide identity between strain NBT06-6T and C. imitans were 24.5±2.4 and 81.9 %, respectively.
29557772	10	41	theme	24.5±2.4 and	1438:1449	arg1	identity					1385:1392	the average nucleotide identity	1362:1392	the average nucleotide identity between strain NBT06-6T and C. imitans	1362:1431	The digital DNA-DNA hybridization value and the average nucleotide identity between strain NBT06-6T and C. imitans were 24.5±2.4 and 81.9 %, respectively.
29557772	2	42	theme	novel	93:97	arg1	bacterium					174:182	A novel heterotrophic, Gram-stain-positive, facultatively anaerobic and rod-shaped bacterium	91:182	A novel heterotrophic, Gram-stain-positive, facultatively anaerobic and rod-shaped bacterium	91:182	A novel heterotrophic, Gram-stain-positive, facultatively anaerobic and rod-shaped bacterium, designated strain NBT06-6T, was isolated from the deep seawater in the New Britain Trench and characterized phylogenetically and phenotypically.
29557772	8	43	from	arabinose	1219:1227	arg1	wall					1180:1183	its cell wall	1171:1183	its cell wall	1171:1183	Strain NBT06-6T contained meso-diaminopimelic acid and mycolic acids in its cell wall, and mannose, galactose, glucose, arabinose and ribose as major whole-cell sugars.
29557772	2	44	theme	anaerobic	149:157	arg1	bacterium					174:182	A novel heterotrophic, Gram-stain-positive, facultatively anaerobic and rod-shaped bacterium	91:182	A novel heterotrophic, Gram-stain-positive, facultatively anaerobic and rod-shaped bacterium	91:182	A novel heterotrophic, Gram-stain-positive, facultatively anaerobic and rod-shaped bacterium, designated strain NBT06-6T, was isolated from the deep seawater in the New Britain Trench and characterized phylogenetically and phenotypically.
29557772	4	45	theme	strain	592:597	arg1	NBT06-6T					599:606	strain NBT06-6T	592:606	strain NBT06-6T	592:606	The strain grew at hydrostatic pressures of 0.1-100 MPa (optimum, 0.1 MPa) at 35 °C. Phylogenetic analysis of the 16S rRNA gene sequences indicated that strain NBT06-6T belonged to the genus Corynebacterium, with the highest sequence similarity (97.9 %) to Corynebacterium imitans, and shared low 16S rRNA gene sequence similarities (<97.0 %) with other type strains.
29557772	9	46	theme	genomic	1291:1297	arg1	DNA					1299:1301	the genomic DNA	1287:1301	the genomic DNA	1287:1301	The G+C content of the genomic DNA was 65.1 mol%.
29557772	4	47	theme	rRNA	740:743	arg1	similarities					759:770	low 16S rRNA gene sequence similarities	732:770	low 16S rRNA gene sequence similarities	732:770	The strain grew at hydrostatic pressures of 0.1-100 MPa (optimum, 0.1 MPa) at 35 °C. Phylogenetic analysis of the 16S rRNA gene sequences indicated that strain NBT06-6T belonged to the genus Corynebacterium, with the highest sequence similarity (97.9 %) to Corynebacterium imitans, and shared low 16S rRNA gene sequence similarities (<97.0 %) with other type strains.
29557772	4	48	theme	sequences	567:575	arg1	analysis					537:544	Phylogenetic analysis	524:544	Phylogenetic analysis of the 16S rRNA gene sequences	524:575	The strain grew at hydrostatic pressures of 0.1-100 MPa (optimum, 0.1 MPa) at 35 °C. Phylogenetic analysis of the 16S rRNA gene sequences indicated that strain NBT06-6T belonged to the genus Corynebacterium, with the highest sequence similarity (97.9 %) to Corynebacterium imitans, and shared low 16S rRNA gene sequence similarities (<97.0 %) with other type strains.
29557772	11	49	theme	strain	1531:1536	arg1	NBT06-6T					1538:1545	strain NBT06-6T	1531:1545	strain NBT06-6T	1531:1545	The combined genotypic and phenotypic data indicated that strain NBT06-6T represents a novel species of the genus Corynebacterium, for which the name Corynebacterium hadale sp.
29557772	4	50	theme	sequence	750:757	arg1	similarities					759:770	low 16S rRNA gene sequence similarities	732:770	low 16S rRNA gene sequence similarities	732:770	The strain grew at hydrostatic pressures of 0.1-100 MPa (optimum, 0.1 MPa) at 35 °C. Phylogenetic analysis of the 16S rRNA gene sequences indicated that strain NBT06-6T belonged to the genus Corynebacterium, with the highest sequence similarity (97.9 %) to Corynebacterium imitans, and shared low 16S rRNA gene sequence similarities (<97.0 %) with other type strains.
29557772	2	51	theme	deep	235:238	arg1	seawater					240:247	the deep seawater	231:247	the deep seawater in the New Britain Trench	231:273	A novel heterotrophic, Gram-stain-positive, facultatively anaerobic and rod-shaped bacterium, designated strain NBT06-6T, was isolated from the deep seawater in the New Britain Trench and characterized phylogenetically and phenotypically.
29557772	4	52	dep	shared	725:730	arg1	%					779:779	<97.0 %	773:779	<97.0 %	773:779	The strain grew at hydrostatic pressures of 0.1-100 MPa (optimum, 0.1 MPa) at 35 °C. Phylogenetic analysis of the 16S rRNA gene sequences indicated that strain NBT06-6T belonged to the genus Corynebacterium, with the highest sequence similarity (97.9 %) to Corynebacterium imitans, and shared low 16S rRNA gene sequence similarities (<97.0 %) with other type strains.
29557772	4	53	theme	rRNA	557:560	arg1	sequences					567:575	the 16S rRNA gene sequences	549:575	the 16S rRNA gene sequences	549:575	The strain grew at hydrostatic pressures of 0.1-100 MPa (optimum, 0.1 MPa) at 35 °C. Phylogenetic analysis of the 16S rRNA gene sequences indicated that strain NBT06-6T belonged to the genus Corynebacterium, with the highest sequence similarity (97.9 %) to Corynebacterium imitans, and shared low 16S rRNA gene sequence similarities (<97.0 %) with other type strains.
29557772	7	54	dep	 0	1083:1084	arg1	 0					1095:1096	 0	1095:1096	 0	1095:1096	The major fatty acids detected were C18 : 1ω9c, C16 : 0 and C15 : 0.
29557772	4	55	theme	genus	624:628	arg1	Corynebacterium					630:644	the genus Corynebacterium	620:644	the genus Corynebacterium	620:644	The strain grew at hydrostatic pressures of 0.1-100 MPa (optimum, 0.1 MPa) at 35 °C. Phylogenetic analysis of the 16S rRNA gene sequences indicated that strain NBT06-6T belonged to the genus Corynebacterium, with the highest sequence similarity (97.9 %) to Corynebacterium imitans, and shared low 16S rRNA gene sequence similarities (<97.0 %) with other type strains.
29557772	2	56	theme	New	256:258	arg1	Trench					268:273	the New Britain Trench	252:273	the New Britain Trench	252:273	A novel heterotrophic, Gram-stain-positive, facultatively anaerobic and rod-shaped bacterium, designated strain NBT06-6T, was isolated from the deep seawater in the New Britain Trench and characterized phylogenetically and phenotypically.
29557772	5	57	theme	major	811:815	arg1	MK-8					847:850	MK-8	847:850	MK-8	847:850	The major respiratory menaquinones were MK-8(H2) and MK-9(H2).
29557772	5	57	theme	major	811:815	arg1	menaquinones					829:840	The major respiratory menaquinones	807:840	The major respiratory menaquinones	807:840	The major respiratory menaquinones were MK-8(H2) and MK-9(H2).
29557772	4	58	theme	type	793:796	arg1	strains					798:804	other type strains	787:804	other type strains	787:804	The strain grew at hydrostatic pressures of 0.1-100 MPa (optimum, 0.1 MPa) at 35 °C. Phylogenetic analysis of the 16S rRNA gene sequences indicated that strain NBT06-6T belonged to the genus Corynebacterium, with the highest sequence similarity (97.9 %) to Corynebacterium imitans, and shared low 16S rRNA gene sequence similarities (<97.0 %) with other type strains.
29557772	8	59	theme	cell	1175:1178	arg1	wall					1180:1183	its cell wall	1171:1183	its cell wall	1171:1183	Strain NBT06-6T contained meso-diaminopimelic acid and mycolic acids in its cell wall, and mannose, galactose, glucose, arabinose and ribose as major whole-cell sugars.
29557772	11	60	theme	name	1618:1621	arg1	sp					1646:1647	the name Corynebacterium hadale sp	1614:1647	the name Corynebacterium hadale sp	1614:1647	The combined genotypic and phenotypic data indicated that strain NBT06-6T represents a novel species of the genus Corynebacterium, for which the name Corynebacterium hadale sp.
29557772	4	61	dep	35 °C.	517:522	arg1	indicated					577:585	indicated	577:585	indicated that strain NBT06-6T belonged to the genus Corynebacterium, with the highest sequence similarity (97.9 %) to Corynebacterium imitans	577:718	The strain grew at hydrostatic pressures of 0.1-100 MPa (optimum, 0.1 MPa) at 35 °C. Phylogenetic analysis of the 16S rRNA gene sequences indicated that strain NBT06-6T belonged to the genus Corynebacterium, with the highest sequence similarity (97.9 %) to Corynebacterium imitans, and shared low 16S rRNA gene sequence similarities (<97.0 %) with other type strains.
29557772	6	62	theme	polar	874:878	arg1	diphosphatidyglycerol					892:912	diphosphatidyglycerol	892:912	diphosphatidyglycerol	892:912	The polar lipids were diphosphatidyglycerol, phosphatidylglycerol, phosphatidylinositol, three unidentified aminoglycolipids and four unidentified glycolipids.
29557772	6	62	theme	polar	874:878	arg1	lipids					880:885	The polar lipids	870:885	The polar lipids	870:885	The polar lipids were diphosphatidyglycerol, phosphatidylglycerol, phosphatidylinositol, three unidentified aminoglycolipids and four unidentified glycolipids.
29557772	8	63	from	acid	1145:1148	arg1	wall					1180:1183	its cell wall	1171:1183	its cell wall	1171:1183	Strain NBT06-6T contained meso-diaminopimelic acid and mycolic acids in its cell wall, and mannose, galactose, glucose, arabinose and ribose as major whole-cell sugars.
29557772	3	64	theme	bacterial	338:346	arg1	growth					348:353	Optimal bacterial growth	330:353	Optimal bacterial growth	330:353	Optimal bacterial growth occurred at 35 °C (range 22-41 °C), at pH 6 (4-8) and with 4 % (w/v) NaCl (0-10 %).
29557772	2	65	attach	isolated	217:224	arg1	seawater					240:247	the deep seawater	231:247	the deep seawater in the New Britain Trench	231:273	A novel heterotrophic, Gram-stain-positive, facultatively anaerobic and rod-shaped bacterium, designated strain NBT06-6T, was isolated from the deep seawater in the New Britain Trench and characterized phylogenetically and phenotypically.
29557772	2	65	attach	isolated	217:224	arg2	bacterium					174:182	A novel heterotrophic, Gram-stain-positive, facultatively anaerobic and rod-shaped bacterium	91:182	A novel heterotrophic, Gram-stain-positive, facultatively anaerobic and rod-shaped bacterium	91:182	A novel heterotrophic, Gram-stain-positive, facultatively anaerobic and rod-shaped bacterium, designated strain NBT06-6T, was isolated from the deep seawater in the New Britain Trench and characterized phylogenetically and phenotypically.
29557772	11	66	theme	hadale	1639:1644	arg1	sp					1646:1647	the name Corynebacterium hadale sp	1614:1647	the name Corynebacterium hadale sp	1614:1647	The combined genotypic and phenotypic data indicated that strain NBT06-6T represents a novel species of the genus Corynebacterium, for which the name Corynebacterium hadale sp.
29557772	6	67	theme	unidentified	965:976	arg1	aminoglycolipids					978:993	three unidentified aminoglycolipids	959:993	three unidentified aminoglycolipids	959:993	The polar lipids were diphosphatidyglycerol, phosphatidylglycerol, phosphatidylinositol, three unidentified aminoglycolipids and four unidentified glycolipids.
29557772	8	68	from	mannose	1190:1196	arg1	wall					1180:1183	its cell wall	1171:1183	its cell wall	1171:1183	Strain NBT06-6T contained meso-diaminopimelic acid and mycolic acids in its cell wall, and mannose, galactose, glucose, arabinose and ribose as major whole-cell sugars.
29557772	1	69	theme	Britain	75:81	arg1	Trench					83:88	the New Britain Trench	67:88	the New Britain Trench	67:88	nov. isolated from hadopelagic water of the New Britain Trench.
29557772	4	70	theme	0.1-100 MPa	483:493	arg1	pressures					470:478	hydrostatic pressures	458:478	hydrostatic pressures of 0.1-100 MPa (optimum, 0.1 MPa)	458:512	The strain grew at hydrostatic pressures of 0.1-100 MPa (optimum, 0.1 MPa) at 35 °C. Phylogenetic analysis of the 16S rRNA gene sequences indicated that strain NBT06-6T belonged to the genus Corynebacterium, with the highest sequence similarity (97.9 %) to Corynebacterium imitans, and shared low 16S rRNA gene sequence similarities (<97.0 %) with other type strains.
29557772	2	71	theme	strain	196:201	arg1	NBT06-6T					203:210	strain NBT06-6T	196:210	strain NBT06-6T	196:210	A novel heterotrophic, Gram-stain-positive, facultatively anaerobic and rod-shaped bacterium, designated strain NBT06-6T, was isolated from the deep seawater in the New Britain Trench and characterized phylogenetically and phenotypically.
29557772	2	72	from	seawater	240:247	arg1	Trench					268:273	the New Britain Trench	252:273	the New Britain Trench	252:273	A novel heterotrophic, Gram-stain-positive, facultatively anaerobic and rod-shaped bacterium, designated strain NBT06-6T, was isolated from the deep seawater in the New Britain Trench and characterized phylogenetically and phenotypically.
29557772	4	73	theme	sequence	664:671	arg1	similarity					673:682	the highest sequence similarity	652:682	the highest sequence similarity (97.9 %) to Corynebacterium imitans	652:718	The strain grew at hydrostatic pressures of 0.1-100 MPa (optimum, 0.1 MPa) at 35 °C. Phylogenetic analysis of the 16S rRNA gene sequences indicated that strain NBT06-6T belonged to the genus Corynebacterium, with the highest sequence similarity (97.9 %) to Corynebacterium imitans, and shared low 16S rRNA gene sequence similarities (<97.0 %) with other type strains.
29557772	4	73	theme	sequence	664:671	arg1	%					690:690	97.9 %	685:690	97.9 %	685:690	The strain grew at hydrostatic pressures of 0.1-100 MPa (optimum, 0.1 MPa) at 35 °C. Phylogenetic analysis of the 16S rRNA gene sequences indicated that strain NBT06-6T belonged to the genus Corynebacterium, with the highest sequence similarity (97.9 %) to Corynebacterium imitans, and shared low 16S rRNA gene sequence similarities (<97.0 %) with other type strains.
29557772	10	74	theme	DNA-DNA	1330:1336	arg1	hybridization					1338:1350	The digital DNA-DNA hybridization	1318:1350	The digital DNA-DNA hybridization value	1318:1356	The digital DNA-DNA hybridization value and the average nucleotide identity between strain NBT06-6T and C. imitans were 24.5±2.4 and 81.9 %, respectively.
29557772	8	75	theme	major	1243:1247	arg1	sugars					1260:1265	major whole-cell sugars	1243:1265	major whole-cell sugars	1243:1265	Strain NBT06-6T contained meso-diaminopimelic acid and mycolic acids in its cell wall, and mannose, galactose, glucose, arabinose and ribose as major whole-cell sugars.
29557772	7	76	theme	fatty	1040:1044	arg1	acids					1046:1050	The major fatty acids	1030:1050	The major fatty acids detected	1030:1059	The major fatty acids detected were C18 : 1ω9c, C16 : 0 and C15 : 0.
29557772	7	76	theme	fatty	1040:1044	arg1	C18 					1066:1069	C18 	1066:1069	C18 	1066:1069	The major fatty acids detected were C18 : 1ω9c, C16 : 0 and C15 : 0.
29557772	10	77	theme	nucleotide	1374:1383	arg1	%					1456:1456	24.5±2.4 and 81.9 %	1438:1456	24.5±2.4 and 81.9 %	1438:1456	The digital DNA-DNA hybridization value and the average nucleotide identity between strain NBT06-6T and C. imitans were 24.5±2.4 and 81.9 %, respectively.
29557772	10	77	theme	nucleotide	1374:1383	arg1	identity					1385:1392	the average nucleotide identity	1362:1392	the average nucleotide identity between strain NBT06-6T and C. imitans	1362:1431	The digital DNA-DNA hybridization value and the average nucleotide identity between strain NBT06-6T and C. imitans were 24.5±2.4 and 81.9 %, respectively.
29557772	11	78	theme	Corynebacterium	1587:1601	arg1	species					1566:1572	a novel species	1558:1572	a novel species	1558:1572	The combined genotypic and phenotypic data indicated that strain NBT06-6T represents a novel species of the genus Corynebacterium, for which the name Corynebacterium hadale sp.
29557772	4	79	theme	low	732:734	arg1	similarities					759:770	low 16S rRNA gene sequence similarities	732:770	low 16S rRNA gene sequence similarities	732:770	The strain grew at hydrostatic pressures of 0.1-100 MPa (optimum, 0.1 MPa) at 35 °C. Phylogenetic analysis of the 16S rRNA gene sequences indicated that strain NBT06-6T belonged to the genus Corynebacterium, with the highest sequence similarity (97.9 %) to Corynebacterium imitans, and shared low 16S rRNA gene sequence similarities (<97.0 %) with other type strains.
29557772	12	80	theme	1K03347T=DSM	1705:1716	arg1	NBT06-6T					1689:1696	the type strain NBT06-6T	1673:1696	the type strain NBT06-6T (=MCCC 1K03347T=DSM 105365T)	1673:1725	nov. is proposed, with the type strain NBT06-6T (=MCCC 1K03347T=DSM 105365T).
29557772	12	80	theme	1K03347T=DSM	1705:1716	arg1	105365T					1718:1724	=MCCC 1K03347T=DSM 105365T	1699:1724	=MCCC 1K03347T=DSM 105365T	1699:1724	nov. is proposed, with the type strain NBT06-6T (=MCCC 1K03347T=DSM 105365T).
29557772	12	81	theme	type	1677:1680	arg1	NBT06-6T					1689:1696	the type strain NBT06-6T	1673:1696	the type strain NBT06-6T (=MCCC 1K03347T=DSM 105365T)	1673:1725	nov. is proposed, with the type strain NBT06-6T (=MCCC 1K03347T=DSM 105365T).
29557772	12	81	theme	type	1677:1680	arg1	105365T					1718:1724	=MCCC 1K03347T=DSM 105365T	1699:1724	=MCCC 1K03347T=DSM 105365T	1699:1724	nov. is proposed, with the type strain NBT06-6T (=MCCC 1K03347T=DSM 105365T).
29557772	4	82	theme	Phylogenetic	524:535	arg1	analysis					537:544	Phylogenetic analysis	524:544	Phylogenetic analysis of the 16S rRNA gene sequences	524:575	The strain grew at hydrostatic pressures of 0.1-100 MPa (optimum, 0.1 MPa) at 35 °C. Phylogenetic analysis of the 16S rRNA gene sequences indicated that strain NBT06-6T belonged to the genus Corynebacterium, with the highest sequence similarity (97.9 %) to Corynebacterium imitans, and shared low 16S rRNA gene sequence similarities (<97.0 %) with other type strains.
29557772	11	83	theme	combined	1477:1484	arg1	data					1511:1514	The combined genotypic and phenotypic data	1473:1514	The combined genotypic and phenotypic data	1473:1514	The combined genotypic and phenotypic data indicated that strain NBT06-6T represents a novel species of the genus Corynebacterium, for which the name Corynebacterium hadale sp.
29557772	8	84	theme	mycolic	1154:1160	arg1	acids					1162:1166	mycolic acids	1154:1166	mycolic acids	1154:1166	Strain NBT06-6T contained meso-diaminopimelic acid and mycolic acids in its cell wall, and mannose, galactose, glucose, arabinose and ribose as major whole-cell sugars.
29557772	6	85	theme	unidentified	1004:1015	arg1	glycolipids					1017:1027	four unidentified glycolipids	999:1027	four unidentified glycolipids	999:1027	The polar lipids were diphosphatidyglycerol, phosphatidylglycerol, phosphatidylinositol, three unidentified aminoglycolipids and four unidentified glycolipids.
29557772	2	86	theme	Gram-stain-positive	114:132	arg1	bacterium					174:182	A novel heterotrophic, Gram-stain-positive, facultatively anaerobic and rod-shaped bacterium	91:182	A novel heterotrophic, Gram-stain-positive, facultatively anaerobic and rod-shaped bacterium	91:182	A novel heterotrophic, Gram-stain-positive, facultatively anaerobic and rod-shaped bacterium, designated strain NBT06-6T, was isolated from the deep seawater in the New Britain Trench and characterized phylogenetically and phenotypically.
29869212	0	0	theme	matter	82:87	arg1	production					89:98	extracellular organic matter production	60:98	extracellular organic matter production	60:98	Effects of multiple environmental factors on the growth and extracellular organic matter production of Microcystis aeruginosa: a central composite design response surface model.
29869212	3	1	theme	synergic	721:728	arg1	effects					730:736	synergic effects	721:736	synergic effects on the production of microcystins (MC-LR) and extracellular polysaccharides (EPS)	721:818	The results of the experiments reveal that nitrate and phosphate had significant interactive effects on algal density, both iron and light intensity had synergic effects on the production of microcystins (MC-LR) and extracellular polysaccharides (EPS), and light intensity and nitrite had clear interactive effects on EPS release.
29869212	6	2	theme	organic	1337:1343	arg1	production					1352:1361	extracellular organic matter production	1323:1361	extracellular organic matter production	1323:1361	This study contributes to our theoretical knowledge of the prediction and analysis of M. aeruginosa growth and extracellular organic matter production.
29869212	6	3	theme	matter	1345:1350	arg1	production					1352:1361	extracellular organic matter production	1323:1361	extracellular organic matter production	1323:1361	This study contributes to our theoretical knowledge of the prediction and analysis of M. aeruginosa growth and extracellular organic matter production.
29869212	0	4	theme	organic	74:80	arg1	production					89:98	extracellular organic matter production	60:98	extracellular organic matter production	60:98	Effects of multiple environmental factors on the growth and extracellular organic matter production of Microcystis aeruginosa: a central composite design response surface model.
29869212	3	5	theme	light	701:705	arg1	intensity					707:715	light intensity	701:715	light intensity	701:715	The results of the experiments reveal that nitrate and phosphate had significant interactive effects on algal density, both iron and light intensity had synergic effects on the production of microcystins (MC-LR) and extracellular polysaccharides (EPS), and light intensity and nitrite had clear interactive effects on EPS release.
29869212	6	6	theme	extracellular	1323:1335	arg1	production					1352:1361	extracellular organic matter production	1323:1361	extracellular organic matter production	1323:1361	This study contributes to our theoretical knowledge of the prediction and analysis of M. aeruginosa growth and extracellular organic matter production.
29869212	3	7	contain	had	717:719	arg1	iron					692:695	iron	692:695	iron	692:695	The results of the experiments reveal that nitrate and phosphate had significant interactive effects on algal density, both iron and light intensity had synergic effects on the production of microcystins (MC-LR) and extracellular polysaccharides (EPS), and light intensity and nitrite had clear interactive effects on EPS release.
29869212	3	7	contain	had	717:719	arg2	effects					730:736	synergic effects	721:736	synergic effects on the production of microcystins (MC-LR) and extracellular polysaccharides (EPS)	721:818	The results of the experiments reveal that nitrate and phosphate had significant interactive effects on algal density, both iron and light intensity had synergic effects on the production of microcystins (MC-LR) and extracellular polysaccharides (EPS), and light intensity and nitrite had clear interactive effects on EPS release.
29869212	3	7	contain	had	717:719	arg1	intensity					707:715	light intensity	701:715	light intensity	701:715	The results of the experiments reveal that nitrate and phosphate had significant interactive effects on algal density, both iron and light intensity had synergic effects on the production of microcystins (MC-LR) and extracellular polysaccharides (EPS), and light intensity and nitrite had clear interactive effects on EPS release.
29869212	4	8	from	effects	944:950	arg1	production					1000:1009	extracellular dissolved organic carbon (DOC) production	955:1009	extracellular dissolved organic carbon (DOC) production	955:1009	Results did not show significant interactive effects on extracellular dissolved organic carbon (DOC) production.
29869212	2	9	theme	extracellular	538:550	arg1	matter					560:565	extracellular organic matter	538:565	extracellular organic matter	538:565	A central composite design response surface model was established to investigate the multiple effects of various physical and chemical factors (total nitrogen, total phosphorus, temperature, and light intensity) on algal density and extracellular organic matter.
29869212	0	10	from	Effects	0:6	arg1	production					89:98	extracellular organic matter production	60:98	extracellular organic matter production	60:98	Effects of multiple environmental factors on the growth and extracellular organic matter production of Microcystis aeruginosa: a central composite design response surface model.
29869212	0	10	from	Effects	0:6	arg1	growth					49:54	growth	49:54	growth	49:54	Effects of multiple environmental factors on the growth and extracellular organic matter production of Microcystis aeruginosa: a central composite design response surface model.
29869212	2	11	theme	central	307:313	arg1	model					349:353	A central composite design response surface model	305:353	A central composite design response surface model	305:353	A central composite design response surface model was established to investigate the multiple effects of various physical and chemical factors (total nitrogen, total phosphorus, temperature, and light intensity) on algal density and extracellular organic matter.
29869212	4	12	theme	dissolved	969:977	arg1	DOC					995:997	DOC	995:997	DOC	995:997	Results did not show significant interactive effects on extracellular dissolved organic carbon (DOC) production.
29869212	4	12	theme	dissolved	969:977	arg1	carbon					987:992	dissolved organic carbon	969:992	extracellular dissolved organic carbon (DOC) production	955:1009	Results did not show significant interactive effects on extracellular dissolved organic carbon (DOC) production.
29869212	6	13	dep	growth	1312:1317	arg1	aeruginosa					1301:1310	M. aeruginosa growth and extracellular organic matter production	1298:1361	M. aeruginosa growth and extracellular organic matter production	1298:1361	This study contributes to our theoretical knowledge of the prediction and analysis of M. aeruginosa growth and extracellular organic matter production.
29869212	6	14	theme	growth	1312:1317	arg1	analysis					1286:1293	analysis	1286:1293	analysis of M. aeruginosa growth and extracellular organic matter production	1286:1361	This study contributes to our theoretical knowledge of the prediction and analysis of M. aeruginosa growth and extracellular organic matter production.
29869212	6	14	theme	growth	1312:1317	arg1	knowledge					1254:1262	our theoretical knowledge	1238:1262	our theoretical knowledge of the prediction	1238:1280	This study contributes to our theoretical knowledge of the prediction and analysis of M. aeruginosa growth and extracellular organic matter production.
29869212	6	15	theme	theoretical	1242:1252	arg1	knowledge					1254:1262	our theoretical knowledge	1238:1262	our theoretical knowledge of the prediction	1238:1280	This study contributes to our theoretical knowledge of the prediction and analysis of M. aeruginosa growth and extracellular organic matter production.
29869212	3	16	theme	experiments	587:597	arg1	results					572:578	The results	568:578	The results of the experiments	568:597	The results of the experiments reveal that nitrate and phosphate had significant interactive effects on algal density, both iron and light intensity had synergic effects on the production of microcystins (MC-LR) and extracellular polysaccharides (EPS), and light intensity and nitrite had clear interactive effects on EPS release.
29869212	0	17	theme	aeruginosa	115:124	arg1	production					89:98	extracellular organic matter production	60:98	extracellular organic matter production	60:98	Effects of multiple environmental factors on the growth and extracellular organic matter production of Microcystis aeruginosa: a central composite design response surface model.
29869212	0	17	theme	aeruginosa	115:124	arg1	growth					49:54	growth	49:54	growth	49:54	Effects of multiple environmental factors on the growth and extracellular organic matter production of Microcystis aeruginosa: a central composite design response surface model.
29869212	3	18	contain	had	853:855	arg1	nitrite					845:851	nitrite	845:851	nitrite	845:851	The results of the experiments reveal that nitrate and phosphate had significant interactive effects on algal density, both iron and light intensity had synergic effects on the production of microcystins (MC-LR) and extracellular polysaccharides (EPS), and light intensity and nitrite had clear interactive effects on EPS release.
29869212	3	18	contain	had	853:855	arg1	intensity					831:839	light intensity	825:839	light intensity	825:839	The results of the experiments reveal that nitrate and phosphate had significant interactive effects on algal density, both iron and light intensity had synergic effects on the production of microcystins (MC-LR) and extracellular polysaccharides (EPS), and light intensity and nitrite had clear interactive effects on EPS release.
29869212	3	18	contain	had	853:855	arg2	effects					875:881	clear interactive effects	857:881	clear interactive effects	857:881	The results of the experiments reveal that nitrate and phosphate had significant interactive effects on algal density, both iron and light intensity had synergic effects on the production of microcystins (MC-LR) and extracellular polysaccharides (EPS), and light intensity and nitrite had clear interactive effects on EPS release.
29869212	4	19	theme	interactive	932:942	arg1	effects					944:950	significant interactive effects	920:950	significant interactive effects on extracellular dissolved organic carbon (DOC) production	920:1009	Results did not show significant interactive effects on extracellular dissolved organic carbon (DOC) production.
29869212	2	20	theme	algal	520:524	arg1	density					526:532	algal density	520:532	algal density	520:532	A central composite design response surface model was established to investigate the multiple effects of various physical and chemical factors (total nitrogen, total phosphorus, temperature, and light intensity) on algal density and extracellular organic matter.
29869212	6	21	theme	M.	1298:1299	arg1	growth					1312:1317	growth	1312:1317	growth	1312:1317	This study contributes to our theoretical knowledge of the prediction and analysis of M. aeruginosa growth and extracellular organic matter production.
29869212	2	22	theme	physical	418:425	arg1	factors					440:446	various physical and chemical factors	410:446	various physical and chemical factors (total nitrogen, total phosphorus, temperature, and light intensity)	410:515	A central composite design response surface model was established to investigate the multiple effects of various physical and chemical factors (total nitrogen, total phosphorus, temperature, and light intensity) on algal density and extracellular organic matter.
29869212	2	22	theme	physical	418:425	arg1	nitrogen					455:462	total nitrogen	449:462	total nitrogen	449:462	A central composite design response surface model was established to investigate the multiple effects of various physical and chemical factors (total nitrogen, total phosphorus, temperature, and light intensity) on algal density and extracellular organic matter.
29869212	2	22	theme	physical	418:425	arg1	phosphorus					471:480	total phosphorus	465:480	total phosphorus	465:480	A central composite design response surface model was established to investigate the multiple effects of various physical and chemical factors (total nitrogen, total phosphorus, temperature, and light intensity) on algal density and extracellular organic matter.
29869212	2	22	theme	physical	418:425	arg1	intensity					506:514	light intensity	500:514	light intensity	500:514	A central composite design response surface model was established to investigate the multiple effects of various physical and chemical factors (total nitrogen, total phosphorus, temperature, and light intensity) on algal density and extracellular organic matter.
29869212	2	22	theme	physical	418:425	arg1	temperature					483:493	temperature	483:493	temperature	483:493	A central composite design response surface model was established to investigate the multiple effects of various physical and chemical factors (total nitrogen, total phosphorus, temperature, and light intensity) on algal density and extracellular organic matter.
29869212	3	23	from	effects	730:736	arg1	production					745:754	the production	741:754	the production of microcystins (MC-LR) and extracellular polysaccharides (EPS)	741:818	The results of the experiments reveal that nitrate and phosphate had significant interactive effects on algal density, both iron and light intensity had synergic effects on the production of microcystins (MC-LR) and extracellular polysaccharides (EPS), and light intensity and nitrite had clear interactive effects on EPS release.
29869212	2	24	theme	light	500:504	arg1	factors					440:446	various physical and chemical factors	410:446	various physical and chemical factors (total nitrogen, total phosphorus, temperature, and light intensity)	410:515	A central composite design response surface model was established to investigate the multiple effects of various physical and chemical factors (total nitrogen, total phosphorus, temperature, and light intensity) on algal density and extracellular organic matter.
29869212	2	24	theme	light	500:504	arg1	intensity					506:514	light intensity	500:514	light intensity	500:514	A central composite design response surface model was established to investigate the multiple effects of various physical and chemical factors (total nitrogen, total phosphorus, temperature, and light intensity) on algal density and extracellular organic matter.
29869212	2	25	theme	surface	341:347	arg1	model					349:353	A central composite design response surface model	305:353	A central composite design response surface model	305:353	A central composite design response surface model was established to investigate the multiple effects of various physical and chemical factors (total nitrogen, total phosphorus, temperature, and light intensity) on algal density and extracellular organic matter.
29869212	3	26	theme	light	825:829	arg1	intensity					831:839	light intensity	825:839	light intensity	825:839	The results of the experiments reveal that nitrate and phosphate had significant interactive effects on algal density, both iron and light intensity had synergic effects on the production of microcystins (MC-LR) and extracellular polysaccharides (EPS), and light intensity and nitrite had clear interactive effects on EPS release.
29869212	2	27	theme	various	410:416	arg1	factors					440:446	various physical and chemical factors	410:446	various physical and chemical factors (total nitrogen, total phosphorus, temperature, and light intensity)	410:515	A central composite design response surface model was established to investigate the multiple effects of various physical and chemical factors (total nitrogen, total phosphorus, temperature, and light intensity) on algal density and extracellular organic matter.
29869212	2	27	theme	various	410:416	arg1	nitrogen					455:462	total nitrogen	449:462	total nitrogen	449:462	A central composite design response surface model was established to investigate the multiple effects of various physical and chemical factors (total nitrogen, total phosphorus, temperature, and light intensity) on algal density and extracellular organic matter.
29869212	2	27	theme	various	410:416	arg1	phosphorus					471:480	total phosphorus	465:480	total phosphorus	465:480	A central composite design response surface model was established to investigate the multiple effects of various physical and chemical factors (total nitrogen, total phosphorus, temperature, and light intensity) on algal density and extracellular organic matter.
29869212	2	27	theme	various	410:416	arg1	intensity					506:514	light intensity	500:514	light intensity	500:514	A central composite design response surface model was established to investigate the multiple effects of various physical and chemical factors (total nitrogen, total phosphorus, temperature, and light intensity) on algal density and extracellular organic matter.
29869212	2	27	theme	various	410:416	arg1	temperature					483:493	temperature	483:493	temperature	483:493	A central composite design response surface model was established to investigate the multiple effects of various physical and chemical factors (total nitrogen, total phosphorus, temperature, and light intensity) on algal density and extracellular organic matter.
29869212	2	28	theme	total	465:469	arg1	factors					440:446	various physical and chemical factors	410:446	various physical and chemical factors (total nitrogen, total phosphorus, temperature, and light intensity)	410:515	A central composite design response surface model was established to investigate the multiple effects of various physical and chemical factors (total nitrogen, total phosphorus, temperature, and light intensity) on algal density and extracellular organic matter.
29869212	2	28	theme	total	465:469	arg1	phosphorus					471:480	total phosphorus	465:480	total phosphorus	465:480	A central composite design response surface model was established to investigate the multiple effects of various physical and chemical factors (total nitrogen, total phosphorus, temperature, and light intensity) on algal density and extracellular organic matter.
29869212	2	29	from	effects	399:405	arg1	density					526:532	algal density	520:532	algal density	520:532	A central composite design response surface model was established to investigate the multiple effects of various physical and chemical factors (total nitrogen, total phosphorus, temperature, and light intensity) on algal density and extracellular organic matter.
29869212	2	29	from	effects	399:405	arg1	matter					560:565	extracellular organic matter	538:565	extracellular organic matter	538:565	A central composite design response surface model was established to investigate the multiple effects of various physical and chemical factors (total nitrogen, total phosphorus, temperature, and light intensity) on algal density and extracellular organic matter.
29869212	2	30	theme	response	332:339	arg1	model					349:353	A central composite design response surface model	305:353	A central composite design response surface model	305:353	A central composite design response surface model was established to investigate the multiple effects of various physical and chemical factors (total nitrogen, total phosphorus, temperature, and light intensity) on algal density and extracellular organic matter.
29869212	0	31	theme	environmental	20:32	arg1	factors					34:40	multiple environmental factors	11:40	multiple environmental factors	11:40	Effects of multiple environmental factors on the growth and extracellular organic matter production of Microcystis aeruginosa: a central composite design response surface model.
29869212	4	32	theme	significant	920:930	arg1	effects					944:950	significant interactive effects	920:950	significant interactive effects on extracellular dissolved organic carbon (DOC) production	920:1009	Results did not show significant interactive effects on extracellular dissolved organic carbon (DOC) production.
29869212	5	33	theme	organic	1056:1062	arg1	matter					1064:1069	extracellular dissolved organic matter	1032:1069	extracellular dissolved organic matter of Microcystis aeruginosa	1032:1095	The contribution of extracellular dissolved organic matter of Microcystis aeruginosa during the logarithmic phase was further identified using a three-dimensional excitation emission matrix (3-DEEM).
29869212	2	34	theme	design	325:330	arg1	model					349:353	A central composite design response surface model	305:353	A central composite design response surface model	305:353	A central composite design response surface model was established to investigate the multiple effects of various physical and chemical factors (total nitrogen, total phosphorus, temperature, and light intensity) on algal density and extracellular organic matter.
29869212	2	35	theme	total	449:453	arg1	factors					440:446	various physical and chemical factors	410:446	various physical and chemical factors (total nitrogen, total phosphorus, temperature, and light intensity)	410:515	A central composite design response surface model was established to investigate the multiple effects of various physical and chemical factors (total nitrogen, total phosphorus, temperature, and light intensity) on algal density and extracellular organic matter.
29869212	2	35	theme	total	449:453	arg1	nitrogen					455:462	total nitrogen	449:462	total nitrogen	449:462	A central composite design response surface model was established to investigate the multiple effects of various physical and chemical factors (total nitrogen, total phosphorus, temperature, and light intensity) on algal density and extracellular organic matter.
29869212	0	36	theme	multiple	11:18	arg1	factors					34:40	multiple environmental factors	11:40	multiple environmental factors	11:40	Effects of multiple environmental factors on the growth and extracellular organic matter production of Microcystis aeruginosa: a central composite design response surface model.
29869212	5	37	theme	three-dimensional	1157:1173	arg1	3-DEEM					1203:1208	3-DEEM	1203:1208	3-DEEM	1203:1208	The contribution of extracellular dissolved organic matter of Microcystis aeruginosa during the logarithmic phase was further identified using a three-dimensional excitation emission matrix (3-DEEM).
29869212	5	37	theme	three-dimensional	1157:1173	arg1	matrix					1195:1200	a three-dimensional excitation emission matrix	1155:1200	a three-dimensional excitation emission matrix (3-DEEM)	1155:1209	The contribution of extracellular dissolved organic matter of Microcystis aeruginosa during the logarithmic phase was further identified using a three-dimensional excitation emission matrix (3-DEEM).
29869212	3	38	theme	algal	672:676	arg1	density					678:684	algal density	672:684	algal density	672:684	The results of the experiments reveal that nitrate and phosphate had significant interactive effects on algal density, both iron and light intensity had synergic effects on the production of microcystins (MC-LR) and extracellular polysaccharides (EPS), and light intensity and nitrite had clear interactive effects on EPS release.
29869212	2	39	theme	composite	315:323	arg1	model					349:353	A central composite design response surface model	305:353	A central composite design response surface model	305:353	A central composite design response surface model was established to investigate the multiple effects of various physical and chemical factors (total nitrogen, total phosphorus, temperature, and light intensity) on algal density and extracellular organic matter.
29869212	3	40	theme	microcystins	759:770	arg1	production					745:754	the production	741:754	the production of microcystins (MC-LR) and extracellular polysaccharides (EPS)	741:818	The results of the experiments reveal that nitrate and phosphate had significant interactive effects on algal density, both iron and light intensity had synergic effects on the production of microcystins (MC-LR) and extracellular polysaccharides (EPS), and light intensity and nitrite had clear interactive effects on EPS release.
29869212	0	41	theme	composite	137:145	arg1	model					171:175	a central composite design response surface model	127:175	Effects of multiple environmental factors on the growth and extracellular organic matter production of Microcystis aeruginosa: a central composite design response surface model.	0:176	Effects of multiple environmental factors on the growth and extracellular organic matter production of Microcystis aeruginosa: a central composite design response surface model.
29869212	1	42	theme	designed	207:214	arg1	experiments					216:226	statistically designed experiments	193:226	statistically designed experiments using response surface methodology	193:261	In this study, statistically designed experiments using response surface methodology were conducted on Microcystis aeruginosa.
29869212	2	43	dep	factors	440:446	arg1	temperature					483:493	temperature	483:493	temperature	483:493	A central composite design response surface model was established to investigate the multiple effects of various physical and chemical factors (total nitrogen, total phosphorus, temperature, and light intensity) on algal density and extracellular organic matter.
29869212	2	43	dep	factors	440:446	arg1	nitrogen					455:462	total nitrogen	449:462	total nitrogen	449:462	A central composite design response surface model was established to investigate the multiple effects of various physical and chemical factors (total nitrogen, total phosphorus, temperature, and light intensity) on algal density and extracellular organic matter.
29869212	2	43	dep	factors	440:446	arg1	phosphorus					471:480	total phosphorus	465:480	total phosphorus	465:480	A central composite design response surface model was established to investigate the multiple effects of various physical and chemical factors (total nitrogen, total phosphorus, temperature, and light intensity) on algal density and extracellular organic matter.
29869212	2	43	dep	factors	440:446	arg1	intensity					506:514	light intensity	500:514	light intensity	500:514	A central composite design response surface model was established to investigate the multiple effects of various physical and chemical factors (total nitrogen, total phosphorus, temperature, and light intensity) on algal density and extracellular organic matter.
29869212	2	43	dep	factors	440:446	arg1	factors					440:446	various physical and chemical factors	410:446	various physical and chemical factors (total nitrogen, total phosphorus, temperature, and light intensity)	410:515	A central composite design response surface model was established to investigate the multiple effects of various physical and chemical factors (total nitrogen, total phosphorus, temperature, and light intensity) on algal density and extracellular organic matter.
29869212	2	44	theme	factors	440:446	arg1	effects					399:405	the multiple effects	386:405	the multiple effects of various physical and chemical factors (total nitrogen, total phosphorus, temperature, and light intensity) on algal density and extracellular organic matter	386:565	A central composite design response surface model was established to investigate the multiple effects of various physical and chemical factors (total nitrogen, total phosphorus, temperature, and light intensity) on algal density and extracellular organic matter.
29869212	0	45	theme	factors	34:40	arg1	Effects					0:6	Effects	0:6	Effects of multiple environmental factors on the growth and extracellular organic matter production of Microcystis aeruginosa: a central composite design response surface model.	0:176	Effects of multiple environmental factors on the growth and extracellular organic matter production of Microcystis aeruginosa: a central composite design response surface model.
29869212	3	46	theme	extracellular	784:796	arg1	EPS					815:817	EPS	815:817	EPS	815:817	The results of the experiments reveal that nitrate and phosphate had significant interactive effects on algal density, both iron and light intensity had synergic effects on the production of microcystins (MC-LR) and extracellular polysaccharides (EPS), and light intensity and nitrite had clear interactive effects on EPS release.
29869212	3	46	theme	extracellular	784:796	arg1	polysaccharides					798:812	extracellular polysaccharides	784:812	extracellular polysaccharides (EPS)	784:818	The results of the experiments reveal that nitrate and phosphate had significant interactive effects on algal density, both iron and light intensity had synergic effects on the production of microcystins (MC-LR) and extracellular polysaccharides (EPS), and light intensity and nitrite had clear interactive effects on EPS release.
29869212	0	47	theme	central	129:135	arg1	model					171:175	a central composite design response surface model	127:175	Effects of multiple environmental factors on the growth and extracellular organic matter production of Microcystis aeruginosa: a central composite design response surface model.	0:176	Effects of multiple environmental factors on the growth and extracellular organic matter production of Microcystis aeruginosa: a central composite design response surface model.
29869212	5	48	theme	logarithmic	1108:1118	arg1	phase					1120:1124	the logarithmic phase	1104:1124	the logarithmic phase	1104:1124	The contribution of extracellular dissolved organic matter of Microcystis aeruginosa during the logarithmic phase was further identified using a three-dimensional excitation emission matrix (3-DEEM).
29869212	5	49	theme	extracellular	1032:1044	arg1	matter					1064:1069	extracellular dissolved organic matter	1032:1069	extracellular dissolved organic matter of Microcystis aeruginosa	1032:1095	The contribution of extracellular dissolved organic matter of Microcystis aeruginosa during the logarithmic phase was further identified using a three-dimensional excitation emission matrix (3-DEEM).
29869212	2	50	theme	chemical	431:438	arg1	factors					440:446	various physical and chemical factors	410:446	various physical and chemical factors (total nitrogen, total phosphorus, temperature, and light intensity)	410:515	A central composite design response surface model was established to investigate the multiple effects of various physical and chemical factors (total nitrogen, total phosphorus, temperature, and light intensity) on algal density and extracellular organic matter.
29869212	2	50	theme	chemical	431:438	arg1	nitrogen					455:462	total nitrogen	449:462	total nitrogen	449:462	A central composite design response surface model was established to investigate the multiple effects of various physical and chemical factors (total nitrogen, total phosphorus, temperature, and light intensity) on algal density and extracellular organic matter.
29869212	2	50	theme	chemical	431:438	arg1	phosphorus					471:480	total phosphorus	465:480	total phosphorus	465:480	A central composite design response surface model was established to investigate the multiple effects of various physical and chemical factors (total nitrogen, total phosphorus, temperature, and light intensity) on algal density and extracellular organic matter.
29869212	2	50	theme	chemical	431:438	arg1	intensity					506:514	light intensity	500:514	light intensity	500:514	A central composite design response surface model was established to investigate the multiple effects of various physical and chemical factors (total nitrogen, total phosphorus, temperature, and light intensity) on algal density and extracellular organic matter.
29869212	2	50	theme	chemical	431:438	arg1	temperature					483:493	temperature	483:493	temperature	483:493	A central composite design response surface model was established to investigate the multiple effects of various physical and chemical factors (total nitrogen, total phosphorus, temperature, and light intensity) on algal density and extracellular organic matter.
29869212	3	51	theme	polysaccharides	798:812	arg1	production					745:754	the production	741:754	the production of microcystins (MC-LR) and extracellular polysaccharides (EPS)	741:818	The results of the experiments reveal that nitrate and phosphate had significant interactive effects on algal density, both iron and light intensity had synergic effects on the production of microcystins (MC-LR) and extracellular polysaccharides (EPS), and light intensity and nitrite had clear interactive effects on EPS release.
29869212	0	52	theme	response	154:161	arg1	model					171:175	a central composite design response surface model	127:175	Effects of multiple environmental factors on the growth and extracellular organic matter production of Microcystis aeruginosa: a central composite design response surface model.	0:176	Effects of multiple environmental factors on the growth and extracellular organic matter production of Microcystis aeruginosa: a central composite design response surface model.
29869212	5	53	theme	dissolved	1046:1054	arg1	matter					1064:1069	extracellular dissolved organic matter	1032:1069	extracellular dissolved organic matter of Microcystis aeruginosa	1032:1095	The contribution of extracellular dissolved organic matter of Microcystis aeruginosa during the logarithmic phase was further identified using a three-dimensional excitation emission matrix (3-DEEM).
29869212	5	54	theme	excitation	1175:1184	arg1	3-DEEM					1203:1208	3-DEEM	1203:1208	3-DEEM	1203:1208	The contribution of extracellular dissolved organic matter of Microcystis aeruginosa during the logarithmic phase was further identified using a three-dimensional excitation emission matrix (3-DEEM).
29869212	5	54	theme	excitation	1175:1184	arg1	matrix					1195:1200	a three-dimensional excitation emission matrix	1155:1200	a three-dimensional excitation emission matrix (3-DEEM)	1155:1209	The contribution of extracellular dissolved organic matter of Microcystis aeruginosa during the logarithmic phase was further identified using a three-dimensional excitation emission matrix (3-DEEM).
29869212	2	55	theme	organic	552:558	arg1	matter					560:565	extracellular organic matter	538:565	extracellular organic matter	538:565	A central composite design response surface model was established to investigate the multiple effects of various physical and chemical factors (total nitrogen, total phosphorus, temperature, and light intensity) on algal density and extracellular organic matter.
29869212	0	56	theme	design	147:152	arg1	model					171:175	a central composite design response surface model	127:175	Effects of multiple environmental factors on the growth and extracellular organic matter production of Microcystis aeruginosa: a central composite design response surface model.	0:176	Effects of multiple environmental factors on the growth and extracellular organic matter production of Microcystis aeruginosa: a central composite design response surface model.
29869212	5	57	theme	matter	1064:1069	arg1	contribution					1016:1027	The contribution	1012:1027	The contribution of extracellular dissolved organic matter of Microcystis aeruginosa during the logarithmic phase	1012:1124	The contribution of extracellular dissolved organic matter of Microcystis aeruginosa during the logarithmic phase was further identified using a three-dimensional excitation emission matrix (3-DEEM).
29869212	4	58	theme	extracellular	955:967	arg1	production					1000:1009	extracellular dissolved organic carbon (DOC) production	955:1009	extracellular dissolved organic carbon (DOC) production	955:1009	Results did not show significant interactive effects on extracellular dissolved organic carbon (DOC) production.
29869212	3	59	theme	clear	857:861	arg1	effects					875:881	clear interactive effects	857:881	clear interactive effects	857:881	The results of the experiments reveal that nitrate and phosphate had significant interactive effects on algal density, both iron and light intensity had synergic effects on the production of microcystins (MC-LR) and extracellular polysaccharides (EPS), and light intensity and nitrite had clear interactive effects on EPS release.
29869212	6	60	theme	production	1352:1361	arg1	analysis					1286:1293	analysis	1286:1293	analysis of M. aeruginosa growth and extracellular organic matter production	1286:1361	This study contributes to our theoretical knowledge of the prediction and analysis of M. aeruginosa growth and extracellular organic matter production.
29869212	6	60	theme	production	1352:1361	arg1	knowledge					1254:1262	our theoretical knowledge	1238:1262	our theoretical knowledge of the prediction	1238:1280	This study contributes to our theoretical knowledge of the prediction and analysis of M. aeruginosa growth and extracellular organic matter production.
29869212	3	61	theme	significant	637:647	arg1	effects					661:667	significant interactive effects	637:667	significant interactive effects	637:667	The results of the experiments reveal that nitrate and phosphate had significant interactive effects on algal density, both iron and light intensity had synergic effects on the production of microcystins (MC-LR) and extracellular polysaccharides (EPS), and light intensity and nitrite had clear interactive effects on EPS release.
29869212	6	62	theme	prediction	1271:1280	arg1	analysis					1286:1293	analysis	1286:1293	analysis of M. aeruginosa growth and extracellular organic matter production	1286:1361	This study contributes to our theoretical knowledge of the prediction and analysis of M. aeruginosa growth and extracellular organic matter production.
29869212	6	62	theme	prediction	1271:1280	arg1	knowledge					1254:1262	our theoretical knowledge	1238:1262	our theoretical knowledge of the prediction	1238:1280	This study contributes to our theoretical knowledge of the prediction and analysis of M. aeruginosa growth and extracellular organic matter production.
29869212	3	63	theme	interactive	863:873	arg1	effects					875:881	clear interactive effects	857:881	clear interactive effects	857:881	The results of the experiments reveal that nitrate and phosphate had significant interactive effects on algal density, both iron and light intensity had synergic effects on the production of microcystins (MC-LR) and extracellular polysaccharides (EPS), and light intensity and nitrite had clear interactive effects on EPS release.
29869212	3	64	contain	had	633:635	arg1	nitrate					611:617	nitrate	611:617	nitrate	611:617	The results of the experiments reveal that nitrate and phosphate had significant interactive effects on algal density, both iron and light intensity had synergic effects on the production of microcystins (MC-LR) and extracellular polysaccharides (EPS), and light intensity and nitrite had clear interactive effects on EPS release.
29869212	3	64	contain	had	633:635	arg2	effects					661:667	significant interactive effects	637:667	significant interactive effects	637:667	The results of the experiments reveal that nitrate and phosphate had significant interactive effects on algal density, both iron and light intensity had synergic effects on the production of microcystins (MC-LR) and extracellular polysaccharides (EPS), and light intensity and nitrite had clear interactive effects on EPS release.
29869212	3	64	contain	had	633:635	arg1	phosphate					623:631	phosphate	623:631	phosphate	623:631	The results of the experiments reveal that nitrate and phosphate had significant interactive effects on algal density, both iron and light intensity had synergic effects on the production of microcystins (MC-LR) and extracellular polysaccharides (EPS), and light intensity and nitrite had clear interactive effects on EPS release.
29869212	0	65	theme	surface	163:169	arg1	model					171:175	a central composite design response surface model	127:175	Effects of multiple environmental factors on the growth and extracellular organic matter production of Microcystis aeruginosa: a central composite design response surface model.	0:176	Effects of multiple environmental factors on the growth and extracellular organic matter production of Microcystis aeruginosa: a central composite design response surface model.
29869212	3	66	theme	interactive	649:659	arg1	effects					661:667	significant interactive effects	637:667	significant interactive effects	637:667	The results of the experiments reveal that nitrate and phosphate had significant interactive effects on algal density, both iron and light intensity had synergic effects on the production of microcystins (MC-LR) and extracellular polysaccharides (EPS), and light intensity and nitrite had clear interactive effects on EPS release.
29869212	1	67	theme	response	234:241	arg1	methodology					251:261	response surface methodology	234:261	response surface methodology	234:261	In this study, statistically designed experiments using response surface methodology were conducted on Microcystis aeruginosa.
29869212	0	68	theme	extracellular	60:72	arg1	production					89:98	extracellular organic matter production	60:98	extracellular organic matter production	60:98	Effects of multiple environmental factors on the growth and extracellular organic matter production of Microcystis aeruginosa: a central composite design response surface model.
29869212	5	69	theme	aeruginosa	1086:1095	arg1	matter					1064:1069	extracellular dissolved organic matter	1032:1069	extracellular dissolved organic matter of Microcystis aeruginosa	1032:1095	The contribution of extracellular dissolved organic matter of Microcystis aeruginosa during the logarithmic phase was further identified using a three-dimensional excitation emission matrix (3-DEEM).
29869212	5	70	theme	emission	1186:1193	arg1	3-DEEM					1203:1208	3-DEEM	1203:1208	3-DEEM	1203:1208	The contribution of extracellular dissolved organic matter of Microcystis aeruginosa during the logarithmic phase was further identified using a three-dimensional excitation emission matrix (3-DEEM).
29869212	5	70	theme	emission	1186:1193	arg1	matrix					1195:1200	a three-dimensional excitation emission matrix	1155:1200	a three-dimensional excitation emission matrix (3-DEEM)	1155:1209	The contribution of extracellular dissolved organic matter of Microcystis aeruginosa during the logarithmic phase was further identified using a three-dimensional excitation emission matrix (3-DEEM).
29869212	1	71	theme	surface	243:249	arg1	methodology					251:261	response surface methodology	234:261	response surface methodology	234:261	In this study, statistically designed experiments using response surface methodology were conducted on Microcystis aeruginosa.
29869212	4	72	theme	carbon	987:992	arg1	production					1000:1009	extracellular dissolved organic carbon (DOC) production	955:1009	extracellular dissolved organic carbon (DOC) production	955:1009	Results did not show significant interactive effects on extracellular dissolved organic carbon (DOC) production.
29869212	0	73	dep	Effects	0:6	arg1	model					171:175	a central composite design response surface model	127:175	Effects of multiple environmental factors on the growth and extracellular organic matter production of Microcystis aeruginosa: a central composite design response surface model.	0:176	Effects of multiple environmental factors on the growth and extracellular organic matter production of Microcystis aeruginosa: a central composite design response surface model.
29869212	2	74	theme	multiple	390:397	arg1	effects					399:405	the multiple effects	386:405	the multiple effects of various physical and chemical factors (total nitrogen, total phosphorus, temperature, and light intensity) on algal density and extracellular organic matter	386:565	A central composite design response surface model was established to investigate the multiple effects of various physical and chemical factors (total nitrogen, total phosphorus, temperature, and light intensity) on algal density and extracellular organic matter.
29869212	3	75	theme	EPS	886:888	arg1	release					890:896	EPS release	886:896	EPS release	886:896	The results of the experiments reveal that nitrate and phosphate had significant interactive effects on algal density, both iron and light intensity had synergic effects on the production of microcystins (MC-LR) and extracellular polysaccharides (EPS), and light intensity and nitrite had clear interactive effects on EPS release.
29869212	4	76	theme	organic	979:985	arg1	DOC					995:997	DOC	995:997	DOC	995:997	Results did not show significant interactive effects on extracellular dissolved organic carbon (DOC) production.
29869212	4	76	theme	organic	979:985	arg1	carbon					987:992	dissolved organic carbon	969:992	extracellular dissolved organic carbon (DOC) production	955:1009	Results did not show significant interactive effects on extracellular dissolved organic carbon (DOC) production.
29567197	0	0	theme	composite	84:92	arg1	thermogel					94:102	tetronic-alginate composite thermogel	66:102	tetronic-alginate composite thermogel	66:102	Bone regeneration in osteoporosis by delivery BMP-2 and PRGF from tetronic-alginate composite thermogel.
29567197	2	1	theme	bone	473:476	arg1	defect					478:483	a calvaria critical bone defect	453:483	a calvaria critical bone defect in OP female rats	453:501	Therefore in the present study a novel injectable thermo-responsive hydrogel loaded with microspheres of 17β-estradiol, microspheres of bone morphogenetic protein-2 (BMP-2) and plasma rich in growth factors (PRGF) was applied locally to regenerate a calvaria critical bone defect in OP female rats.
29567197	2	2	theme	critical	464:471	arg1	defect					478:483	a calvaria critical bone defect	453:483	a calvaria critical bone defect in OP female rats	453:501	Therefore in the present study a novel injectable thermo-responsive hydrogel loaded with microspheres of 17β-estradiol, microspheres of bone morphogenetic protein-2 (BMP-2) and plasma rich in growth factors (PRGF) was applied locally to regenerate a calvaria critical bone defect in OP female rats.
29567197	0	3	from	regeneration	5:16	arg1	osteoporosis					21:32	osteoporosis	21:32	osteoporosis	21:32	Bone regeneration in osteoporosis by delivery BMP-2 and PRGF from tetronic-alginate composite thermogel.
29567197	2	4	theme	protein-2	360:368	arg1	microspheres					325:336	microspheres	325:336	microspheres of bone morphogenetic protein-2 (BMP-2)	325:376	Therefore in the present study a novel injectable thermo-responsive hydrogel loaded with microspheres of 17β-estradiol, microspheres of bone morphogenetic protein-2 (BMP-2) and plasma rich in growth factors (PRGF) was applied locally to regenerate a calvaria critical bone defect in OP female rats.
29567197	2	4	theme	protein-2	360:368	arg1	17β-estradiol					310:322	17β-estradiol	310:322	17β-estradiol	310:322	Therefore in the present study a novel injectable thermo-responsive hydrogel loaded with microspheres of 17β-estradiol, microspheres of bone morphogenetic protein-2 (BMP-2) and plasma rich in growth factors (PRGF) was applied locally to regenerate a calvaria critical bone defect in OP female rats.
29567197	2	4	theme	protein-2	360:368	arg1	plasma					382:387	plasma	382:387	plasma	382:387	Therefore in the present study a novel injectable thermo-responsive hydrogel loaded with microspheres of 17β-estradiol, microspheres of bone morphogenetic protein-2 (BMP-2) and plasma rich in growth factors (PRGF) was applied locally to regenerate a calvaria critical bone defect in OP female rats.
29567197	6	5	theme	non-OP	902:907	arg1	rats					916:919	non-OP and OP rats	902:919	rats	916:919	In vivo evaluation was made in non-OP and OP rats treated with T-A, T-A with microspheres of 17β-estradiol (T-A-βE), T-A-βE prepared with PRGF (T-A-PRGF-βE), T-A-βE with microspheres of BMP-2 (T-A-βE-BMP-2) and the combination of the three (T-A-PRGF-βE-BMP).
29567197	6	6	theme	combination	1086:1096	arg1	microspheres					1041:1052	microspheres	1041:1052	microspheres of BMP-2 (T-A-βE-BMP-2) and the combination of the three (T-A-PRGF-βE-BMP)	1041:1127	In vivo evaluation was made in non-OP and OP rats treated with T-A, T-A with microspheres of 17β-estradiol (T-A-βE), T-A-βE prepared with PRGF (T-A-PRGF-βE), T-A-βE with microspheres of BMP-2 (T-A-βE-BMP-2) and the combination of the three (T-A-PRGF-βE-BMP).
29567197	6	7	theme	BMP-2	1057:1061	arg1	microspheres					1041:1052	microspheres	1041:1052	microspheres of BMP-2 (T-A-βE-BMP-2) and the combination of the three (T-A-PRGF-βE-BMP)	1041:1127	In vivo evaluation was made in non-OP and OP rats treated with T-A, T-A with microspheres of 17β-estradiol (T-A-βE), T-A-βE prepared with PRGF (T-A-PRGF-βE), T-A-βE with microspheres of BMP-2 (T-A-βE-BMP-2) and the combination of the three (T-A-PRGF-βE-BMP).
29567197	9	8	theme	non-OP	1383:1388	arg1	groups					1390:1395	the non-OP groups	1379:1395	the non-OP groups	1379:1395	The new bone filling the OP defect was less mineralized than in the non-OP groups.
29567197	2	9	theme	female	491:496	arg1	rats					498:501	OP female rats	488:501	OP female rats	488:501	Therefore in the present study a novel injectable thermo-responsive hydrogel loaded with microspheres of 17β-estradiol, microspheres of bone morphogenetic protein-2 (BMP-2) and plasma rich in growth factors (PRGF) was applied locally to regenerate a calvaria critical bone defect in OP female rats.
29567197	2	10	theme	morphogenetic	346:358	arg1	BMP-2					371:375	BMP-2	371:375	BMP-2	371:375	Therefore in the present study a novel injectable thermo-responsive hydrogel loaded with microspheres of 17β-estradiol, microspheres of bone morphogenetic protein-2 (BMP-2) and plasma rich in growth factors (PRGF) was applied locally to regenerate a calvaria critical bone defect in OP female rats.
29567197	2	10	theme	morphogenetic	346:358	arg1	protein-2					360:368	bone morphogenetic protein-2	341:368	bone morphogenetic protein-2 (BMP-2)	341:376	Therefore in the present study a novel injectable thermo-responsive hydrogel loaded with microspheres of 17β-estradiol, microspheres of bone morphogenetic protein-2 (BMP-2) and plasma rich in growth factors (PRGF) was applied locally to regenerate a calvaria critical bone defect in OP female rats.
29567197	9	11	from	mineralized	1359:1369	arg1	groups					1390:1395	the non-OP groups	1379:1395	the non-OP groups	1379:1395	The new bone filling the OP defect was less mineralized than in the non-OP groups.
29567197	1	12	theme	osteoporotic	163:174	arg1	OP					177:178	OP	177:178	OP	177:178	As the life expectancy of the world population increases, osteoporotic (OP) fracture risk increase.
29567197	1	12	theme	osteoporotic	163:174	arg1	risk					190:193	osteoporotic (OP) fracture risk	163:193	osteoporotic (OP) fracture risk	163:193	As the life expectancy of the world population increases, osteoporotic (OP) fracture risk increase.
29567197	2	13	theme	OP	488:489	arg1	rats					498:501	OP female rats	488:501	OP female rats	488:501	Therefore in the present study a novel injectable thermo-responsive hydrogel loaded with microspheres of 17β-estradiol, microspheres of bone morphogenetic protein-2 (BMP-2) and plasma rich in growth factors (PRGF) was applied locally to regenerate a calvaria critical bone defect in OP female rats.
29567197	2	14	theme	thermo-responsive	255:271	arg1	hydrogel					273:280	a novel injectable thermo-responsive hydrogel	236:280	a novel injectable thermo-responsive hydrogel loaded with microspheres of 17β-estradiol, microspheres of bone morphogenetic protein-2 (BMP-2) and plasma rich in growth factors (PRGF)	236:417	Therefore in the present study a novel injectable thermo-responsive hydrogel loaded with microspheres of 17β-estradiol, microspheres of bone morphogenetic protein-2 (BMP-2) and plasma rich in growth factors (PRGF) was applied locally to regenerate a calvaria critical bone defect in OP female rats.
29567197	2	15	theme	rich	389:392	arg1	17β-estradiol					310:322	17β-estradiol	310:322	17β-estradiol	310:322	Therefore in the present study a novel injectable thermo-responsive hydrogel loaded with microspheres of 17β-estradiol, microspheres of bone morphogenetic protein-2 (BMP-2) and plasma rich in growth factors (PRGF) was applied locally to regenerate a calvaria critical bone defect in OP female rats.
29567197	0	16	theme	tetronic-alginate	66:82	arg1	thermogel					94:102	tetronic-alginate composite thermogel	66:102	tetronic-alginate composite thermogel	66:102	Bone regeneration in osteoporosis by delivery BMP-2 and PRGF from tetronic-alginate composite thermogel.
29567197	5	17	theme	long	854:857	arg1	phase					864:868	a long slow phase	852:868	a long slow phase	852:868	The drugs were released during 6 weeks in one fast phase (three days) followed by a long slow phase.
29567197	2	18	theme	plasma	382:387	arg1	microspheres					294:305	microspheres	294:305	microspheres of 17β-estradiol, microspheres of bone morphogenetic protein-2 (BMP-2) and plasma rich in growth factors (PRGF)	294:417	Therefore in the present study a novel injectable thermo-responsive hydrogel loaded with microspheres of 17β-estradiol, microspheres of bone morphogenetic protein-2 (BMP-2) and plasma rich in growth factors (PRGF) was applied locally to regenerate a calvaria critical bone defect in OP female rats.
29567197	0	19	theme	Bone	0:3	arg1	regeneration					5:16	Bone regeneration	0:16	Bone regeneration in osteoporosis by delivery BMP-2 and PRGF from tetronic-alginate composite thermogel.	0:103	Bone regeneration in osteoporosis by delivery BMP-2 and PRGF from tetronic-alginate composite thermogel.
29567197	6	20	theme	three	1105:1109	arg1	combination					1086:1096	the combination	1082:1096	the combination of the three (T-A-PRGF-βE-BMP)	1082:1127	In vivo evaluation was made in non-OP and OP rats treated with T-A, T-A with microspheres of 17β-estradiol (T-A-βE), T-A-βE prepared with PRGF (T-A-PRGF-βE), T-A-βE with microspheres of BMP-2 (T-A-βE-BMP-2) and the combination of the three (T-A-PRGF-βE-BMP).
29567197	6	20	theme	three	1105:1109	arg1	T-A-βE-BMP-2					1064:1075	T-A-βE-BMP-2	1064:1075	T-A-βE-BMP-2	1064:1075	In vivo evaluation was made in non-OP and OP rats treated with T-A, T-A with microspheres of 17β-estradiol (T-A-βE), T-A-βE prepared with PRGF (T-A-PRGF-βE), T-A-βE with microspheres of BMP-2 (T-A-βE-BMP-2) and the combination of the three (T-A-PRGF-βE-BMP).
29567197	6	20	theme	three	1105:1109	arg1	BMP-2					1057:1061	BMP-2	1057:1061	BMP-2 (T-A-βE-BMP-2)	1057:1076	In vivo evaluation was made in non-OP and OP rats treated with T-A, T-A with microspheres of 17β-estradiol (T-A-βE), T-A-βE prepared with PRGF (T-A-PRGF-βE), T-A-βE with microspheres of BMP-2 (T-A-βE-BMP-2) and the combination of the three (T-A-PRGF-βE-BMP).
29567197	2	21	theme	17β-estradiol	310:322	arg1	microspheres					294:305	microspheres	294:305	microspheres of 17β-estradiol, microspheres of bone morphogenetic protein-2 (BMP-2) and plasma rich in growth factors (PRGF)	294:417	Therefore in the present study a novel injectable thermo-responsive hydrogel loaded with microspheres of 17β-estradiol, microspheres of bone morphogenetic protein-2 (BMP-2) and plasma rich in growth factors (PRGF) was applied locally to regenerate a calvaria critical bone defect in OP female rats.
29567197	5	22	theme	fast	816:819	arg1	phase					821:825	one fast phase	812:825	one fast phase (three days) followed by a long slow phase	812:868	The drugs were released during 6 weeks in one fast phase (three days) followed by a long slow phase.
29567197	5	22	theme	fast	816:819	arg1	days					834:837	three days	828:837	three days	828:837	The drugs were released during 6 weeks in one fast phase (three days) followed by a long slow phase.
29567197	6	23	theme	17β-estradiol	964:976	arg1	microspheres					948:959	microspheres	948:959	microspheres of 17β-estradiol (T-A-βE), T-A-βE prepared with PRGF (T-A-PRGF-βE), T-A-βE with microspheres of BMP-2 (T-A-βE-BMP-2) and the combination of the three (T-A-PRGF-βE-BMP)	948:1127	In vivo evaluation was made in non-OP and OP rats treated with T-A, T-A with microspheres of 17β-estradiol (T-A-βE), T-A-βE prepared with PRGF (T-A-PRGF-βE), T-A-βE with microspheres of BMP-2 (T-A-βE-BMP-2) and the combination of the three (T-A-PRGF-βE-BMP).
29567197	7	24	theme	T-A-βE	1251:1256	arg1	formulation					1258:1268	the T-A-βE formulation	1247:1268	the T-A-βE formulation	1247:1268	After 12 weeks, histological and histomorphometric analyzes showed a synergic effect due to the addition of BMP-2 to the T-A-βE formulation.
29567197	3	25	with	T-A	594:596	arg1	T-A-PRGF					612:619	T-A-PRGF	612:619	T-A-PRGF	612:619	Three systems were characterized: Tetronic® 1307 (T-1307) reinforced with alginate (T-A), T-A with PRGF and T-A-PRGF with microspheres.
29567197	3	25	with	T-A	594:596	arg1	PRGF					603:606	PRGF	603:606	PRGF	603:606	Three systems were characterized: Tetronic® 1307 (T-1307) reinforced with alginate (T-A), T-A with PRGF and T-A-PRGF with microspheres.
29567197	3	25	with	T-A	594:596	arg1	microspheres					626:637	microspheres	626:637	microspheres	626:637	Three systems were characterized: Tetronic® 1307 (T-1307) reinforced with alginate (T-A), T-A with PRGF and T-A-PRGF with microspheres.
29567197	1	26	theme	fracture	181:188	arg1	OP					177:178	OP	177:178	OP	177:178	As the life expectancy of the world population increases, osteoporotic (OP) fracture risk increase.
29567197	1	26	theme	fracture	181:188	arg1	risk					190:193	osteoporotic (OP) fracture risk	163:193	osteoporotic (OP) fracture risk	163:193	As the life expectancy of the world population increases, osteoporotic (OP) fracture risk increase.
29567197	5	27	theme	slow	859:862	arg1	phase					864:868	a long slow phase	852:868	a long slow phase	852:868	The drugs were released during 6 weeks in one fast phase (three days) followed by a long slow phase.
29567197	8	28	theme	bone	1302:1305	arg1	repair					1307:1312	the bone repair	1298:1312	the bone repair	1298:1312	The PRGF did not increased the bone repair.
29567197	9	29	theme	new	1319:1321	arg1	mineralized					1359:1369	mineralized	1359:1369	mineralized	1359:1369	The new bone filling the OP defect was less mineralized than in the non-OP groups.
29567197	9	29	theme	new	1319:1321	arg1	bone					1323:1326	The new bone	1315:1326	The new bone filling the OP defect	1315:1348	The new bone filling the OP defect was less mineralized than in the non-OP groups.
29567197	2	30	theme	calvaria	455:462	arg1	defect					478:483	a calvaria critical bone defect	453:483	a calvaria critical bone defect in OP female rats	453:501	Therefore in the present study a novel injectable thermo-responsive hydrogel loaded with microspheres of 17β-estradiol, microspheres of bone morphogenetic protein-2 (BMP-2) and plasma rich in growth factors (PRGF) was applied locally to regenerate a calvaria critical bone defect in OP female rats.
29567197	6	31	theme	In	871:872	arg1	evaluation					879:888	In vivo evaluation	871:888	In vivo evaluation	871:888	In vivo evaluation was made in non-OP and OP rats treated with T-A, T-A with microspheres of 17β-estradiol (T-A-βE), T-A-βE prepared with PRGF (T-A-PRGF-βE), T-A-βE with microspheres of BMP-2 (T-A-βE-BMP-2) and the combination of the three (T-A-PRGF-βE-BMP).
29567197	9	32	theme	OP	1340:1341	arg1	defect					1343:1348	the OP defect	1336:1348	the OP defect	1336:1348	The new bone filling the OP defect was less mineralized than in the non-OP groups.
29567197	2	33	theme	present	222:228	arg1	study					230:234	the present study	218:234	the present study	218:234	Therefore in the present study a novel injectable thermo-responsive hydrogel loaded with microspheres of 17β-estradiol, microspheres of bone morphogenetic protein-2 (BMP-2) and plasma rich in growth factors (PRGF) was applied locally to regenerate a calvaria critical bone defect in OP female rats.
29567197	7	34	theme	histological	1146:1157	arg1	analyzes					1181:1188	histological and histomorphometric analyzes	1146:1188	histological and histomorphometric analyzes	1146:1188	After 12 weeks, histological and histomorphometric analyzes showed a synergic effect due to the addition of BMP-2 to the T-A-βE formulation.
29567197	7	35	theme	synergic	1199:1206	arg1	effect					1208:1213	a synergic effect	1197:1213	a synergic effect due to the addition of BMP-2 to the T-A-βE formulation	1197:1268	After 12 weeks, histological and histomorphometric analyzes showed a synergic effect due to the addition of BMP-2 to the T-A-βE formulation.
29567197	2	36	theme	growth	397:402	arg1	factors					404:410	growth factors	397:410	growth factors (PRGF)	397:417	Therefore in the present study a novel injectable thermo-responsive hydrogel loaded with microspheres of 17β-estradiol, microspheres of bone morphogenetic protein-2 (BMP-2) and plasma rich in growth factors (PRGF) was applied locally to regenerate a calvaria critical bone defect in OP female rats.
29567197	2	36	theme	growth	397:402	arg1	PRGF					413:416	PRGF	413:416	PRGF	413:416	Therefore in the present study a novel injectable thermo-responsive hydrogel loaded with microspheres of 17β-estradiol, microspheres of bone morphogenetic protein-2 (BMP-2) and plasma rich in growth factors (PRGF) was applied locally to regenerate a calvaria critical bone defect in OP female rats.
29567197	2	37	from	defect	478:483	arg1	rats					498:501	OP female rats	488:501	OP female rats	488:501	Therefore in the present study a novel injectable thermo-responsive hydrogel loaded with microspheres of 17β-estradiol, microspheres of bone morphogenetic protein-2 (BMP-2) and plasma rich in growth factors (PRGF) was applied locally to regenerate a calvaria critical bone defect in OP female rats.
29567197	0	38	dep	delivery	37:44	arg1	PRGF					56:59	PRGF	56:59	PRGF	56:59	Bone regeneration in osteoporosis by delivery BMP-2 and PRGF from tetronic-alginate composite thermogel.
29567197	0	38	dep	delivery	37:44	arg1	BMP-2					46:50	BMP-2	46:50	BMP-2	46:50	Bone regeneration in osteoporosis by delivery BMP-2 and PRGF from tetronic-alginate composite thermogel.
29567197	1	39	theme	life	112:115	arg1	expectancy					117:126	the life expectancy	108:126	the life expectancy of the world population	108:150	As the life expectancy of the world population increases, osteoporotic (OP) fracture risk increase.
29567197	6	40	with	T-A-βE	1029:1034	arg1	microspheres					1041:1052	microspheres	1041:1052	microspheres of BMP-2 (T-A-βE-BMP-2) and the combination of the three (T-A-PRGF-βE-BMP)	1041:1127	In vivo evaluation was made in non-OP and OP rats treated with T-A, T-A with microspheres of 17β-estradiol (T-A-βE), T-A-βE prepared with PRGF (T-A-PRGF-βE), T-A-βE with microspheres of BMP-2 (T-A-βE-BMP-2) and the combination of the three (T-A-PRGF-βE-BMP).
29567197	7	41	theme	BMP-2	1238:1242	arg1	addition					1226:1233	the addition	1222:1233	the addition of BMP-2 to the T-A-βE formulation	1222:1268	After 12 weeks, histological and histomorphometric analyzes showed a synergic effect due to the addition of BMP-2 to the T-A-βE formulation.
29567197	2	42	theme	bone	341:344	arg1	BMP-2					371:375	BMP-2	371:375	BMP-2	371:375	Therefore in the present study a novel injectable thermo-responsive hydrogel loaded with microspheres of 17β-estradiol, microspheres of bone morphogenetic protein-2 (BMP-2) and plasma rich in growth factors (PRGF) was applied locally to regenerate a calvaria critical bone defect in OP female rats.
29567197	2	42	theme	bone	341:344	arg1	protein-2					360:368	bone morphogenetic protein-2	341:368	bone morphogenetic protein-2 (BMP-2)	341:376	Therefore in the present study a novel injectable thermo-responsive hydrogel loaded with microspheres of 17β-estradiol, microspheres of bone morphogenetic protein-2 (BMP-2) and plasma rich in growth factors (PRGF) was applied locally to regenerate a calvaria critical bone defect in OP female rats.
29567197	4	43	theme	microspheres	660:671	arg1	addition					644:651	The addition	640:651	The addition of the microspheres	640:671	The addition of the microspheres increased the viscosity but the temperature for the maximum viscosity did not change (22-24 °C).
29567197	6	44	dep	In	871:872	arg1	vivo					874:877	vivo	874:877	vivo	874:877	In vivo evaluation was made in non-OP and OP rats treated with T-A, T-A with microspheres of 17β-estradiol (T-A-βE), T-A-βE prepared with PRGF (T-A-PRGF-βE), T-A-βE with microspheres of BMP-2 (T-A-βE-BMP-2) and the combination of the three (T-A-PRGF-βE-BMP).
29567197	2	45	theme	injectable	244:253	arg1	hydrogel					273:280	a novel injectable thermo-responsive hydrogel	236:280	a novel injectable thermo-responsive hydrogel loaded with microspheres of 17β-estradiol, microspheres of bone morphogenetic protein-2 (BMP-2) and plasma rich in growth factors (PRGF)	236:417	Therefore in the present study a novel injectable thermo-responsive hydrogel loaded with microspheres of 17β-estradiol, microspheres of bone morphogenetic protein-2 (BMP-2) and plasma rich in growth factors (PRGF) was applied locally to regenerate a calvaria critical bone defect in OP female rats.
29567197	2	46	from	rich	389:392	arg1	factors					404:410	growth factors	397:410	growth factors (PRGF)	397:417	Therefore in the present study a novel injectable thermo-responsive hydrogel loaded with microspheres of 17β-estradiol, microspheres of bone morphogenetic protein-2 (BMP-2) and plasma rich in growth factors (PRGF) was applied locally to regenerate a calvaria critical bone defect in OP female rats.
29567197	2	46	from	rich	389:392	arg1	PRGF					413:416	PRGF	413:416	PRGF	413:416	Therefore in the present study a novel injectable thermo-responsive hydrogel loaded with microspheres of 17β-estradiol, microspheres of bone morphogenetic protein-2 (BMP-2) and plasma rich in growth factors (PRGF) was applied locally to regenerate a calvaria critical bone defect in OP female rats.
29567197	4	47	theme	maximum	725:731	arg1	viscosity					733:741	the maximum viscosity	721:741	the maximum viscosity	721:741	The addition of the microspheres increased the viscosity but the temperature for the maximum viscosity did not change (22-24 °C).
29567197	2	48	theme	novel	238:242	arg1	hydrogel					273:280	a novel injectable thermo-responsive hydrogel	236:280	a novel injectable thermo-responsive hydrogel loaded with microspheres of 17β-estradiol, microspheres of bone morphogenetic protein-2 (BMP-2) and plasma rich in growth factors (PRGF)	236:417	Therefore in the present study a novel injectable thermo-responsive hydrogel loaded with microspheres of 17β-estradiol, microspheres of bone morphogenetic protein-2 (BMP-2) and plasma rich in growth factors (PRGF) was applied locally to regenerate a calvaria critical bone defect in OP female rats.
29567197	2	49	from	factors	404:410	arg1	rich					389:392	rich	389:392	rich	389:392	Therefore in the present study a novel injectable thermo-responsive hydrogel loaded with microspheres of 17β-estradiol, microspheres of bone morphogenetic protein-2 (BMP-2) and plasma rich in growth factors (PRGF) was applied locally to regenerate a calvaria critical bone defect in OP female rats.
29567197	2	50	theme	microspheres	325:336	arg1	microspheres					294:305	microspheres	294:305	microspheres of 17β-estradiol, microspheres of bone morphogenetic protein-2 (BMP-2) and plasma rich in growth factors (PRGF)	294:417	Therefore in the present study a novel injectable thermo-responsive hydrogel loaded with microspheres of 17β-estradiol, microspheres of bone morphogenetic protein-2 (BMP-2) and plasma rich in growth factors (PRGF) was applied locally to regenerate a calvaria critical bone defect in OP female rats.
29567197	0	51	from	thermogel	94:102	arg1	delivery					37:44	delivery BMP-2 and PRGF	37:59	delivery BMP-2 and PRGF from tetronic-alginate composite thermogel	37:102	Bone regeneration in osteoporosis by delivery BMP-2 and PRGF from tetronic-alginate composite thermogel.
29567197	7	52	theme	due	1215:1217	arg1	effect					1208:1213	a synergic effect	1197:1213	a synergic effect due to the addition of BMP-2 to the T-A-βE formulation	1197:1268	After 12 weeks, histological and histomorphometric analyzes showed a synergic effect due to the addition of BMP-2 to the T-A-βE formulation.
29567197	7	53	theme	histomorphometric	1163:1179	arg1	analyzes					1181:1188	histological and histomorphometric analyzes	1146:1188	histological and histomorphometric analyzes	1146:1188	After 12 weeks, histological and histomorphometric analyzes showed a synergic effect due to the addition of BMP-2 to the T-A-βE formulation.
29567197	1	54	theme	world	135:139	arg1	population					141:150	the world population	131:150	the world population	131:150	As the life expectancy of the world population increases, osteoporotic (OP) fracture risk increase.
29567197	4	55	dep	change	751:756	arg1	22-24 °C					759:766	22-24 °C	759:766	22-24 °C	759:766	The addition of the microspheres increased the viscosity but the temperature for the maximum viscosity did not change (22-24 °C).
29567197	1	56	theme	population	141:150	arg1	expectancy					117:126	the life expectancy	108:126	the life expectancy of the world population	108:150	As the life expectancy of the world population increases, osteoporotic (OP) fracture risk increase.
29567197	9	57	from	groups	1390:1395	arg1	mineralized					1359:1369	mineralized	1359:1369	mineralized	1359:1369	The new bone filling the OP defect was less mineralized than in the non-OP groups.
29567197	9	57	from	groups	1390:1395	arg1	bone					1323:1326	The new bone	1315:1326	The new bone filling the OP defect	1315:1348	The new bone filling the OP defect was less mineralized than in the non-OP groups.
29567197	6	58	theme	OP	913:914	arg1	rats					916:919	non-OP and OP rats	902:919	rats	916:919	In vivo evaluation was made in non-OP and OP rats treated with T-A, T-A with microspheres of 17β-estradiol (T-A-βE), T-A-βE prepared with PRGF (T-A-PRGF-βE), T-A-βE with microspheres of BMP-2 (T-A-βE-BMP-2) and the combination of the three (T-A-PRGF-βE-BMP).
30556802	7	0	theme	DNA	1374:1376	arg1	%					1390:1390	72.3 mol%	1382:1390	72.3 mol%	1382:1390	The DNA G+C content of genomic DNA was 72.3 mol%.
30556802	7	0	theme	DNA	1374:1376	arg1	G+C content					1351:1361	The DNA G+C content	1343:1361	The DNA G+C content of genomic DNA	1343:1376	The DNA G+C content of genomic DNA was 72.3 mol%.
30556802	4	1	from	presence	442:449	arg1	membranes					767:775	its membranes	763:775	its membranes	763:775	The taxonomic affiliation of this strain at genus level could be confirmed by its chemotaxonomic characteristic, i.e. the presence of ll-diaminopimelic acid in the cell peptidoglycan, MK-9(H4) as the major menaquinone, iso-C16 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0 as the predominant fatty acids in cells, and the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside in its membranes.
30556802	4	1	from	presence	442:449	arg1	peptidoglycan					489:501	the cell peptidoglycan	480:501	the cell peptidoglycan	480:501	The taxonomic affiliation of this strain at genus level could be confirmed by its chemotaxonomic characteristic, i.e. the presence of ll-diaminopimelic acid in the cell peptidoglycan, MK-9(H4) as the major menaquinone, iso-C16 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0 as the predominant fatty acids in cells, and the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside in its membranes.
30556802	4	2	attach	presence	646:653	arg1	membranes					767:775	its membranes	763:775	its membranes	763:775	The taxonomic affiliation of this strain at genus level could be confirmed by its chemotaxonomic characteristic, i.e. the presence of ll-diaminopimelic acid in the cell peptidoglycan, MK-9(H4) as the major menaquinone, iso-C16 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0 as the predominant fatty acids in cells, and the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside in its membranes.
30556802	4	2	attach	presence	646:653	arg2	diphosphatidylglycerol					658:679	diphosphatidylglycerol	658:679	diphosphatidylglycerol	658:679	The taxonomic affiliation of this strain at genus level could be confirmed by its chemotaxonomic characteristic, i.e. the presence of ll-diaminopimelic acid in the cell peptidoglycan, MK-9(H4) as the major menaquinone, iso-C16 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0 as the predominant fatty acids in cells, and the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside in its membranes.
30556802	4	2	attach	presence	646:653	arg2	acid					472:475	ll-diaminopimelic acid	454:475	ll-diaminopimelic acid in the cell peptidoglycan	454:501	The taxonomic affiliation of this strain at genus level could be confirmed by its chemotaxonomic characteristic, i.e. the presence of ll-diaminopimelic acid in the cell peptidoglycan, MK-9(H4) as the major menaquinone, iso-C16 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0 as the predominant fatty acids in cells, and the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside in its membranes.
30556802	4	2	attach	presence	646:653	arg2	MK-9					504:507	MK-9	504:507	MK-9	504:507	The taxonomic affiliation of this strain at genus level could be confirmed by its chemotaxonomic characteristic, i.e. the presence of ll-diaminopimelic acid in the cell peptidoglycan, MK-9(H4) as the major menaquinone, iso-C16 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0 as the predominant fatty acids in cells, and the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside in its membranes.
30556802	4	2	attach	presence	646:653	arg2	phosphatidylglycerol					682:701	phosphatidylglycerol	682:701	phosphatidylglycerol	682:701	The taxonomic affiliation of this strain at genus level could be confirmed by its chemotaxonomic characteristic, i.e. the presence of ll-diaminopimelic acid in the cell peptidoglycan, MK-9(H4) as the major menaquinone, iso-C16 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0 as the predominant fatty acids in cells, and the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside in its membranes.
30556802	4	2	attach	presence	646:653	arg2	phosphatidylinositol					704:723	phosphatidylinositol	704:723	phosphatidylinositol	704:723	The taxonomic affiliation of this strain at genus level could be confirmed by its chemotaxonomic characteristic, i.e. the presence of ll-diaminopimelic acid in the cell peptidoglycan, MK-9(H4) as the major menaquinone, iso-C16 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0 as the predominant fatty acids in cells, and the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside in its membranes.
30556802	4	2	attach	presence	646:653	arg2	mannoside					750:758	phosphatidylinositol mannoside	729:758	phosphatidylinositol mannoside	729:758	The taxonomic affiliation of this strain at genus level could be confirmed by its chemotaxonomic characteristic, i.e. the presence of ll-diaminopimelic acid in the cell peptidoglycan, MK-9(H4) as the major menaquinone, iso-C16 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0 as the predominant fatty acids in cells, and the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside in its membranes.
30556802	6	3	theme	strain	1171:1176	arg1	KE2-3T					1178:1183	strain KE2-3T	1171:1183	strain KE2-3T	1171:1183	However, the draft genome sequence of strain KE2-3T exhibited low average nucleotide identity values to the reference strains (85.5-90.2 %), which were well below the 95-96 % species circumscription threshold.
30556802	7	4	theme	DNA	1347:1349	arg1	%					1390:1390	72.3 mol%	1382:1390	72.3 mol%	1382:1390	The DNA G+C content of genomic DNA was 72.3 mol%.
30556802	7	4	theme	DNA	1347:1349	arg1	G+C content					1351:1361	The DNA G+C content	1343:1361	The DNA G+C content of genomic DNA	1343:1376	The DNA G+C content of genomic DNA was 72.3 mol%.
30556802	4	5	theme	chemotaxonomic	402:415	arg1	characteristic					417:430	its chemotaxonomic characteristic	398:430	its chemotaxonomic characteristic	398:430	The taxonomic affiliation of this strain at genus level could be confirmed by its chemotaxonomic characteristic, i.e. the presence of ll-diaminopimelic acid in the cell peptidoglycan, MK-9(H4) as the major menaquinone, iso-C16 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0 as the predominant fatty acids in cells, and the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside in its membranes.
30556802	4	6	from	peptidoglycan	489:501	arg1	presence					442:449	the presence	438:449	the presence of ll-diaminopimelic acid in the cell peptidoglycan, MK-9(H4) as the major menaquinone, iso-C16 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0 as the predominant fatty acids in cells,	438:636	The taxonomic affiliation of this strain at genus level could be confirmed by its chemotaxonomic characteristic, i.e. the presence of ll-diaminopimelic acid in the cell peptidoglycan, MK-9(H4) as the major menaquinone, iso-C16 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0 as the predominant fatty acids in cells, and the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside in its membranes.
30556802	4	6	from	peptidoglycan	489:501	arg1	presence					646:653	the presence	642:653	the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside in its membranes	642:775	The taxonomic affiliation of this strain at genus level could be confirmed by its chemotaxonomic characteristic, i.e. the presence of ll-diaminopimelic acid in the cell peptidoglycan, MK-9(H4) as the major menaquinone, iso-C16 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0 as the predominant fatty acids in cells, and the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside in its membranes.
30556802	6	7	theme	 species	1307:1314	arg1	threshold					1332:1340	the 95-96 % species circumscription threshold	1296:1340	the 95-96 % species circumscription threshold	1296:1340	However, the draft genome sequence of strain KE2-3T exhibited low average nucleotide identity values to the reference strains (85.5-90.2 %), which were well below the 95-96 % species circumscription threshold.
30556802	4	8	theme	fatty	616:620	arg1	acids					622:626	the predominant fatty acids	600:626	the predominant fatty acids in cells	600:635	The taxonomic affiliation of this strain at genus level could be confirmed by its chemotaxonomic characteristic, i.e. the presence of ll-diaminopimelic acid in the cell peptidoglycan, MK-9(H4) as the major menaquinone, iso-C16 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0 as the predominant fatty acids in cells, and the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside in its membranes.
30556802	1	9	attach	isolated	59:66	arg2	actinomycete					46:57	an endophytic actinomycete	32:57	an endophytic actinomycete isolated from the rhizome of Kaempferia elegans	32:105	nov., an endophytic actinomycete isolated from the rhizome of Kaempferia elegans.
30556802	1	9	attach	isolated	59:66	arg1	rhizome					77:83	the rhizome	73:83	the rhizome of Kaempferia elegans	73:105	nov., an endophytic actinomycete isolated from the rhizome of Kaempferia elegans.
30556802	3	10	theme	taxonomic	285:293	arg1	position					295:302	the taxonomic position	281:302	the taxonomic position of this strain	281:317	The polyphasic approach was used for evaluating the taxonomic position of this strain.
30556802	4	11	from	acid	472:475	arg1	peptidoglycan					489:501	the cell peptidoglycan	480:501	the cell peptidoglycan	480:501	The taxonomic affiliation of this strain at genus level could be confirmed by its chemotaxonomic characteristic, i.e. the presence of ll-diaminopimelic acid in the cell peptidoglycan, MK-9(H4) as the major menaquinone, iso-C16 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0 as the predominant fatty acids in cells, and the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside in its membranes.
30556802	5	12	theme	sequence	801:808	arg1	analysis					810:817	16S rRNA gene sequence analysis	787:817	16S rRNA gene sequence analysis	787:817	Based on 16S rRNA gene sequence analysis, strain KE2-3T was identified as a member of the genus Jiangella and showed the highest similarities to Jiangella muralis DSM 45357T (99.3 %) followed by Jiangella albaDSM 45237T (99.2 %), Jiangella alkaliphilia DSM 45079T (99.0 %), Jiangella gansuensisDSM 44835T (98.8 %) and Jiangella mangrovi3SM4-07T (98.6 %).
30556802	9	13	theme	novel	1575:1579	arg1	species					1581:1587	a novel species	1573:1587	a novel species	1573:1587	Thus, strain KE2-3T should be recognised as a novel species of genus Jiangella, for which the name Jiangellaendophytica sp.
30556802	9	13	theme	novel	1575:1579	arg1	KE2-3T					1542:1547	strain KE2-3T	1535:1547	strain KE2-3T	1535:1547	Thus, strain KE2-3T should be recognised as a novel species of genus Jiangella, for which the name Jiangellaendophytica sp.
30556802	6	14	theme	draft	1146:1150	arg1	sequence					1159:1166	the draft genome sequence	1142:1166	the draft genome sequence of strain KE2-3T	1142:1183	However, the draft genome sequence of strain KE2-3T exhibited low average nucleotide identity values to the reference strains (85.5-90.2 %), which were well below the 95-96 % species circumscription threshold.
30556802	6	15	theme	identity	1218:1225	arg1	values					1227:1232	low average nucleotide identity values	1195:1232	low average nucleotide identity values	1195:1232	However, the draft genome sequence of strain KE2-3T exhibited low average nucleotide identity values to the reference strains (85.5-90.2 %), which were well below the 95-96 % species circumscription threshold.
30556802	6	16	theme	%	1306:1306	arg1	threshold					1332:1340	the 95-96 % species circumscription threshold	1296:1340	the 95-96 % species circumscription threshold	1296:1340	However, the draft genome sequence of strain KE2-3T exhibited low average nucleotide identity values to the reference strains (85.5-90.2 %), which were well below the 95-96 % species circumscription threshold.
30556802	3	17	theme	strain	312:317	arg1	position					295:302	the taxonomic position	281:302	the taxonomic position of this strain	281:317	The polyphasic approach was used for evaluating the taxonomic position of this strain.
30556802	4	18	theme	acid	472:475	arg1	presence					442:449	the presence	438:449	the presence of ll-diaminopimelic acid in the cell peptidoglycan, MK-9(H4) as the major menaquinone, iso-C16 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0 as the predominant fatty acids in cells,	438:636	The taxonomic affiliation of this strain at genus level could be confirmed by its chemotaxonomic characteristic, i.e. the presence of ll-diaminopimelic acid in the cell peptidoglycan, MK-9(H4) as the major menaquinone, iso-C16 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0 as the predominant fatty acids in cells, and the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside in its membranes.
30556802	4	18	theme	acid	472:475	arg1	presence					646:653	the presence	642:653	the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside in its membranes	642:775	The taxonomic affiliation of this strain at genus level could be confirmed by its chemotaxonomic characteristic, i.e. the presence of ll-diaminopimelic acid in the cell peptidoglycan, MK-9(H4) as the major menaquinone, iso-C16 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0 as the predominant fatty acids in cells, and the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside in its membranes.
30556802	11	19	theme	type	1675:1678	arg1	strain					1680:1685	The type strain	1671:1685	The type strain	1671:1685	The type strain is KE2-3T (=BCC 66359T=NBRC 110004T).
30556802	11	19	theme	type	1675:1678	arg1	KE2-3T					1690:1695	KE2-3T	1690:1695	KE2-3T (=BCC 66359T=NBRC 110004T)	1690:1722	The type strain is KE2-3T (=BCC 66359T=NBRC 110004T).
30556802	6	20	theme	average	1199:1205	arg1	identity					1218:1225	average nucleotide identity	1199:1225	low average nucleotide identity values	1195:1232	However, the draft genome sequence of strain KE2-3T exhibited low average nucleotide identity values to the reference strains (85.5-90.2 %), which were well below the 95-96 % species circumscription threshold.
30556802	5	21	theme	albaDSM	983:989	arg1	45237T					991:996	Jiangella albaDSM 45237T	973:996	Jiangella albaDSM 45237T (99.2 %)	973:1005	Based on 16S rRNA gene sequence analysis, strain KE2-3T was identified as a member of the genus Jiangella and showed the highest similarities to Jiangella muralis DSM 45357T (99.3 %) followed by Jiangella albaDSM 45237T (99.2 %), Jiangella alkaliphilia DSM 45079T (99.0 %), Jiangella gansuensisDSM 44835T (98.8 %) and Jiangella mangrovi3SM4-07T (98.6 %).
30556802	5	21	theme	albaDSM	983:989	arg1	%					1004:1004	99.2 %	999:1004	99.2 %	999:1004	Based on 16S rRNA gene sequence analysis, strain KE2-3T was identified as a member of the genus Jiangella and showed the highest similarities to Jiangella muralis DSM 45357T (99.3 %) followed by Jiangella albaDSM 45237T (99.2 %), Jiangella alkaliphilia DSM 45079T (99.0 %), Jiangella gansuensisDSM 44835T (98.8 %) and Jiangella mangrovi3SM4-07T (98.6 %).
30556802	2	22	theme	elegans	224:230	arg1	rhizome					202:208	surface-sterilised rhizome	183:208	surface-sterilised rhizome of Kaempferia elegans	183:230	An endophytic actinobacterium, designated strain KE2-3T, was isolated from surface-sterilised rhizome of Kaempferia elegans.
30556802	5	23	theme	genus	868:872	arg1	Jiangella					874:882	the genus Jiangella	864:882	the genus Jiangella	864:882	Based on 16S rRNA gene sequence analysis, strain KE2-3T was identified as a member of the genus Jiangella and showed the highest similarities to Jiangella muralis DSM 45357T (99.3 %) followed by Jiangella albaDSM 45237T (99.2 %), Jiangella alkaliphilia DSM 45079T (99.0 %), Jiangella gansuensisDSM 44835T (98.8 %) and Jiangella mangrovi3SM4-07T (98.6 %).
30556802	9	24	theme	Jiangella	1598:1606	arg1	species					1581:1587	a novel species	1573:1587	a novel species	1573:1587	Thus, strain KE2-3T should be recognised as a novel species of genus Jiangella, for which the name Jiangellaendophytica sp.
30556802	9	24	theme	Jiangella	1598:1606	arg1	KE2-3T					1542:1547	strain KE2-3T	1535:1547	strain KE2-3T	1535:1547	Thus, strain KE2-3T should be recognised as a novel species of genus Jiangella, for which the name Jiangellaendophytica sp.
30556802	1	25	dep	actinomycete	46:57	arg1	nov.					26:29	nov.	26:29	nov.	26:29	nov., an endophytic actinomycete isolated from the rhizome of Kaempferia elegans.
30556802	5	26	theme	highest	899:905	arg1	member					854:859	a member	852:859	a member of the genus Jiangella	852:882	Based on 16S rRNA gene sequence analysis, strain KE2-3T was identified as a member of the genus Jiangella and showed the highest similarities to Jiangella muralis DSM 45357T (99.3 %) followed by Jiangella albaDSM 45237T (99.2 %), Jiangella alkaliphilia DSM 45079T (99.0 %), Jiangella gansuensisDSM 44835T (98.8 %) and Jiangella mangrovi3SM4-07T (98.6 %).
30556802	5	26	theme	highest	899:905	arg1	KE2-3T					827:832	strain KE2-3T	820:832	strain KE2-3T	820:832	Based on 16S rRNA gene sequence analysis, strain KE2-3T was identified as a member of the genus Jiangella and showed the highest similarities to Jiangella muralis DSM 45357T (99.3 %) followed by Jiangella albaDSM 45237T (99.2 %), Jiangella alkaliphilia DSM 45079T (99.0 %), Jiangella gansuensisDSM 44835T (98.8 %) and Jiangella mangrovi3SM4-07T (98.6 %).
30556802	5	26	theme	highest	899:905	arg1	similarities					907:918	the highest similarities	895:918	the highest similarities to Jiangella muralis DSM 45357T (99.3 %) followed by Jiangella albaDSM 45237T (99.2 %), Jiangella alkaliphilia DSM 45079T (99.0 %), Jiangella gansuensisDSM 44835T (98.8 %) and Jiangella mangrovi3SM4-07T (98.6 %)	895:1130	Based on 16S rRNA gene sequence analysis, strain KE2-3T was identified as a member of the genus Jiangella and showed the highest similarities to Jiangella muralis DSM 45357T (99.3 %) followed by Jiangella albaDSM 45237T (99.2 %), Jiangella alkaliphilia DSM 45079T (99.0 %), Jiangella gansuensisDSM 44835T (98.8 %) and Jiangella mangrovi3SM4-07T (98.6 %).
30556802	5	27	theme	rRNA	791:794	arg1	analysis					810:817	16S rRNA gene sequence analysis	787:817	16S rRNA gene sequence analysis	787:817	Based on 16S rRNA gene sequence analysis, strain KE2-3T was identified as a member of the genus Jiangella and showed the highest similarities to Jiangella muralis DSM 45357T (99.3 %) followed by Jiangella albaDSM 45237T (99.2 %), Jiangella alkaliphilia DSM 45079T (99.0 %), Jiangella gansuensisDSM 44835T (98.8 %) and Jiangella mangrovi3SM4-07T (98.6 %).
30556802	9	28	theme	Jiangellaendophytica	1628:1647	arg1	sp					1649:1650	the name Jiangellaendophytica sp	1619:1650	the name Jiangellaendophytica sp	1619:1650	Thus, strain KE2-3T should be recognised as a novel species of genus Jiangella, for which the name Jiangellaendophytica sp.
30556802	5	29	theme	alkaliphilia	1018:1029	arg1	45079T					1035:1040	Jiangella alkaliphilia DSM 45079T	1008:1040	Jiangella alkaliphilia DSM 45079T (99.0 %)	1008:1049	Based on 16S rRNA gene sequence analysis, strain KE2-3T was identified as a member of the genus Jiangella and showed the highest similarities to Jiangella muralis DSM 45357T (99.3 %) followed by Jiangella albaDSM 45237T (99.2 %), Jiangella alkaliphilia DSM 45079T (99.0 %), Jiangella gansuensisDSM 44835T (98.8 %) and Jiangella mangrovi3SM4-07T (98.6 %).
30556802	5	29	theme	alkaliphilia	1018:1029	arg1	%					1048:1048	99.0 %	1043:1048	99.0 %	1043:1048	Based on 16S rRNA gene sequence analysis, strain KE2-3T was identified as a member of the genus Jiangella and showed the highest similarities to Jiangella muralis DSM 45357T (99.3 %) followed by Jiangella albaDSM 45237T (99.2 %), Jiangella alkaliphilia DSM 45079T (99.0 %), Jiangella gansuensisDSM 44835T (98.8 %) and Jiangella mangrovi3SM4-07T (98.6 %).
30556802	5	30	theme	Jiangella	923:931	arg1	%					958:958	99.3 %	953:958	99.3 %	953:958	Based on 16S rRNA gene sequence analysis, strain KE2-3T was identified as a member of the genus Jiangella and showed the highest similarities to Jiangella muralis DSM 45357T (99.3 %) followed by Jiangella albaDSM 45237T (99.2 %), Jiangella alkaliphilia DSM 45079T (99.0 %), Jiangella gansuensisDSM 44835T (98.8 %) and Jiangella mangrovi3SM4-07T (98.6 %).
30556802	5	30	theme	Jiangella	923:931	arg1	DSM					941:943	Jiangella muralis DSM 45357T	923:950	Jiangella muralis DSM 45357T (99.3 %) followed by Jiangella albaDSM 45237T (99.2 %), Jiangella alkaliphilia DSM 45079T (99.0 %), Jiangella gansuensisDSM 44835T (98.8 %) and Jiangella mangrovi3SM4-07T (98.6 %)	923:1130	Based on 16S rRNA gene sequence analysis, strain KE2-3T was identified as a member of the genus Jiangella and showed the highest similarities to Jiangella muralis DSM 45357T (99.3 %) followed by Jiangella albaDSM 45237T (99.2 %), Jiangella alkaliphilia DSM 45079T (99.0 %), Jiangella gansuensisDSM 44835T (98.8 %) and Jiangella mangrovi3SM4-07T (98.6 %).
30556802	4	31	theme	mannoside	750:758	arg1	presence					442:449	the presence	438:449	the presence of ll-diaminopimelic acid in the cell peptidoglycan, MK-9(H4) as the major menaquinone, iso-C16 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0 as the predominant fatty acids in cells,	438:636	The taxonomic affiliation of this strain at genus level could be confirmed by its chemotaxonomic characteristic, i.e. the presence of ll-diaminopimelic acid in the cell peptidoglycan, MK-9(H4) as the major menaquinone, iso-C16 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0 as the predominant fatty acids in cells, and the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside in its membranes.
30556802	4	31	theme	mannoside	750:758	arg1	presence					646:653	the presence	642:653	the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside in its membranes	642:775	The taxonomic affiliation of this strain at genus level could be confirmed by its chemotaxonomic characteristic, i.e. the presence of ll-diaminopimelic acid in the cell peptidoglycan, MK-9(H4) as the major menaquinone, iso-C16 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0 as the predominant fatty acids in cells, and the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside in its membranes.
30556802	6	32	theme	reference	1241:1249	arg1	strains					1251:1257	the reference strains	1237:1257	the reference strains (85.5-90.2 %)	1237:1271	However, the draft genome sequence of strain KE2-3T exhibited low average nucleotide identity values to the reference strains (85.5-90.2 %), which were well below the 95-96 % species circumscription threshold.
30556802	6	32	theme	reference	1241:1249	arg1	%					1270:1270	85.5-90.2 %	1260:1270	85.5-90.2 %	1260:1270	However, the draft genome sequence of strain KE2-3T exhibited low average nucleotide identity values to the reference strains (85.5-90.2 %), which were well below the 95-96 % species circumscription threshold.
30556802	0	33	theme	Jiangella	0:8	arg1	sp					22:23	Jiangella endophytica sp	0:23	Jiangella endophytica sp.	0:24	Jiangella endophytica sp.
30556802	8	34	theme	physiological	1417:1429	arg1	data					1458:1461	physiological, biochemical and genotypic data	1417:1461	physiological, biochemical and genotypic data	1417:1461	With the differences of physiological, biochemical and genotypic data, strain KE2-3T could be discriminated from its closest neighbour.
30556802	6	35	theme	95-96 	1300:1305	arg1	%					1306:1306	%	1306:1306	%	1306:1306	However, the draft genome sequence of strain KE2-3T exhibited low average nucleotide identity values to the reference strains (85.5-90.2 %), which were well below the 95-96 % species circumscription threshold.
30556802	1	36	theme	elegans	99:105	arg1	rhizome					77:83	the rhizome	73:83	the rhizome of Kaempferia elegans	73:105	nov., an endophytic actinomycete isolated from the rhizome of Kaempferia elegans.
30556802	8	37	theme	biochemical	1432:1442	arg1	data					1458:1461	physiological, biochemical and genotypic data	1417:1461	physiological, biochemical and genotypic data	1417:1461	With the differences of physiological, biochemical and genotypic data, strain KE2-3T could be discriminated from its closest neighbour.
30556802	5	38	theme	Jiangella	1052:1060	arg1	44835T					1076:1081	Jiangella gansuensisDSM 44835T	1052:1081	Jiangella gansuensisDSM 44835T (98.8 %)	1052:1090	Based on 16S rRNA gene sequence analysis, strain KE2-3T was identified as a member of the genus Jiangella and showed the highest similarities to Jiangella muralis DSM 45357T (99.3 %) followed by Jiangella albaDSM 45237T (99.2 %), Jiangella alkaliphilia DSM 45079T (99.0 %), Jiangella gansuensisDSM 44835T (98.8 %) and Jiangella mangrovi3SM4-07T (98.6 %).
30556802	5	38	theme	Jiangella	1052:1060	arg1	%					1089:1089	98.8 %	1084:1089	98.8 %	1084:1089	Based on 16S rRNA gene sequence analysis, strain KE2-3T was identified as a member of the genus Jiangella and showed the highest similarities to Jiangella muralis DSM 45357T (99.3 %) followed by Jiangella albaDSM 45237T (99.2 %), Jiangella alkaliphilia DSM 45079T (99.0 %), Jiangella gansuensisDSM 44835T (98.8 %) and Jiangella mangrovi3SM4-07T (98.6 %).
30556802	4	39	theme	strain	354:359	arg1	affiliation					334:344	The taxonomic affiliation	320:344	The taxonomic affiliation of this strain at genus level	320:374	The taxonomic affiliation of this strain at genus level could be confirmed by its chemotaxonomic characteristic, i.e. the presence of ll-diaminopimelic acid in the cell peptidoglycan, MK-9(H4) as the major menaquinone, iso-C16 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0 as the predominant fatty acids in cells, and the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside in its membranes.
30556802	8	40	theme	genotypic	1448:1456	arg1	data					1458:1461	physiological, biochemical and genotypic data	1417:1461	physiological, biochemical and genotypic data	1417:1461	With the differences of physiological, biochemical and genotypic data, strain KE2-3T could be discriminated from its closest neighbour.
30556802	11	41	theme	66359T=NBRC	1703:1713	arg1	110004T					1715:1721	=BCC 66359T=NBRC 110004T	1698:1721	=BCC 66359T=NBRC 110004T	1698:1721	The type strain is KE2-3T (=BCC 66359T=NBRC 110004T).
30556802	11	41	theme	66359T=NBRC	1703:1713	arg1	KE2-3T					1690:1695	KE2-3T	1690:1695	KE2-3T (=BCC 66359T=NBRC 110004T)	1690:1722	The type strain is KE2-3T (=BCC 66359T=NBRC 110004T).
30556802	5	42	theme	Jiangella	973:981	arg1	45237T					991:996	Jiangella albaDSM 45237T	973:996	Jiangella albaDSM 45237T (99.2 %)	973:1005	Based on 16S rRNA gene sequence analysis, strain KE2-3T was identified as a member of the genus Jiangella and showed the highest similarities to Jiangella muralis DSM 45357T (99.3 %) followed by Jiangella albaDSM 45237T (99.2 %), Jiangella alkaliphilia DSM 45079T (99.0 %), Jiangella gansuensisDSM 44835T (98.8 %) and Jiangella mangrovi3SM4-07T (98.6 %).
30556802	5	42	theme	Jiangella	973:981	arg1	%					1004:1004	99.2 %	999:1004	99.2 %	999:1004	Based on 16S rRNA gene sequence analysis, strain KE2-3T was identified as a member of the genus Jiangella and showed the highest similarities to Jiangella muralis DSM 45357T (99.3 %) followed by Jiangella albaDSM 45237T (99.2 %), Jiangella alkaliphilia DSM 45079T (99.0 %), Jiangella gansuensisDSM 44835T (98.8 %) and Jiangella mangrovi3SM4-07T (98.6 %).
30556802	4	43	theme	genus	364:368	arg1	level					370:374	genus level	364:374	genus level	364:374	The taxonomic affiliation of this strain at genus level could be confirmed by its chemotaxonomic characteristic, i.e. the presence of ll-diaminopimelic acid in the cell peptidoglycan, MK-9(H4) as the major menaquinone, iso-C16 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0 as the predominant fatty acids in cells, and the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside in its membranes.
30556802	4	44	theme	major	520:524	arg1	menaquinone					526:536	the major menaquinone	516:536	the major menaquinone	516:536	The taxonomic affiliation of this strain at genus level could be confirmed by its chemotaxonomic characteristic, i.e. the presence of ll-diaminopimelic acid in the cell peptidoglycan, MK-9(H4) as the major menaquinone, iso-C16 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0 as the predominant fatty acids in cells, and the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside in its membranes.
30556802	4	45	from	presence	646:653	arg1	membranes					767:775	its membranes	763:775	its membranes	763:775	The taxonomic affiliation of this strain at genus level could be confirmed by its chemotaxonomic characteristic, i.e. the presence of ll-diaminopimelic acid in the cell peptidoglycan, MK-9(H4) as the major menaquinone, iso-C16 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0 as the predominant fatty acids in cells, and the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside in its membranes.
30556802	4	45	from	presence	646:653	arg1	peptidoglycan					489:501	the cell peptidoglycan	480:501	the cell peptidoglycan	480:501	The taxonomic affiliation of this strain at genus level could be confirmed by its chemotaxonomic characteristic, i.e. the presence of ll-diaminopimelic acid in the cell peptidoglycan, MK-9(H4) as the major menaquinone, iso-C16 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0 as the predominant fatty acids in cells, and the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside in its membranes.
30556802	4	46	dep	presence	442:449	arg1	i.e.					433:436	i.e.	433:436	i.e.	433:436	The taxonomic affiliation of this strain at genus level could be confirmed by its chemotaxonomic characteristic, i.e. the presence of ll-diaminopimelic acid in the cell peptidoglycan, MK-9(H4) as the major menaquinone, iso-C16 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0 as the predominant fatty acids in cells, and the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside in its membranes.
30556802	4	47	theme	taxonomic	324:332	arg1	affiliation					334:344	The taxonomic affiliation	320:344	The taxonomic affiliation of this strain at genus level	320:374	The taxonomic affiliation of this strain at genus level could be confirmed by its chemotaxonomic characteristic, i.e. the presence of ll-diaminopimelic acid in the cell peptidoglycan, MK-9(H4) as the major menaquinone, iso-C16 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0 as the predominant fatty acids in cells, and the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside in its membranes.
30556802	2	48	theme	surface-sterilised	183:200	arg1	rhizome					202:208	surface-sterilised rhizome	183:208	surface-sterilised rhizome of Kaempferia elegans	183:230	An endophytic actinobacterium, designated strain KE2-3T, was isolated from surface-sterilised rhizome of Kaempferia elegans.
30556802	7	49	theme	genomic	1366:1372	arg1	DNA					1374:1376	genomic DNA	1366:1376	genomic DNA	1366:1376	The DNA G+C content of genomic DNA was 72.3 mol%.
30556802	1	50	theme	endophytic	35:44	arg1	actinomycete					46:57	an endophytic actinomycete	32:57	an endophytic actinomycete isolated from the rhizome of Kaempferia elegans	32:105	nov., an endophytic actinomycete isolated from the rhizome of Kaempferia elegans.
30556802	2	51	theme	endophytic	111:120	arg1	actinobacterium					122:136	An endophytic actinobacterium	108:136	An endophytic actinobacterium	108:136	An endophytic actinobacterium, designated strain KE2-3T, was isolated from surface-sterilised rhizome of Kaempferia elegans.
30556802	6	52	theme	KE2-3T	1178:1183	arg1	sequence					1159:1166	the draft genome sequence	1142:1166	the draft genome sequence of strain KE2-3T	1142:1183	However, the draft genome sequence of strain KE2-3T exhibited low average nucleotide identity values to the reference strains (85.5-90.2 %), which were well below the 95-96 % species circumscription threshold.
30556802	5	53	theme	Jiangella	1096:1104	arg1	mangrovi3SM4-07T					1106:1121	Jiangella mangrovi3SM4-07T	1096:1121	Jiangella mangrovi3SM4-07T (98.6 %)	1096:1130	Based on 16S rRNA gene sequence analysis, strain KE2-3T was identified as a member of the genus Jiangella and showed the highest similarities to Jiangella muralis DSM 45357T (99.3 %) followed by Jiangella albaDSM 45237T (99.2 %), Jiangella alkaliphilia DSM 45079T (99.0 %), Jiangella gansuensisDSM 44835T (98.8 %) and Jiangella mangrovi3SM4-07T (98.6 %).
30556802	5	53	theme	Jiangella	1096:1104	arg1	%					1129:1129	98.6 %	1124:1129	98.6 %	1124:1129	Based on 16S rRNA gene sequence analysis, strain KE2-3T was identified as a member of the genus Jiangella and showed the highest similarities to Jiangella muralis DSM 45357T (99.3 %) followed by Jiangella albaDSM 45237T (99.2 %), Jiangella alkaliphilia DSM 45079T (99.0 %), Jiangella gansuensisDSM 44835T (98.8 %) and Jiangella mangrovi3SM4-07T (98.6 %).
30556802	3	54	theme	polyphasic	237:246	arg1	approach					248:255	The polyphasic approach	233:255	The polyphasic approach	233:255	The polyphasic approach was used for evaluating the taxonomic position of this strain.
30556802	4	55	from	acids	622:626	arg1	cells					631:635	cells	631:635	cells	631:635	The taxonomic affiliation of this strain at genus level could be confirmed by its chemotaxonomic characteristic, i.e. the presence of ll-diaminopimelic acid in the cell peptidoglycan, MK-9(H4) as the major menaquinone, iso-C16 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0 as the predominant fatty acids in cells, and the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside in its membranes.
30556802	9	56	theme	strain	1535:1540	arg1	species					1581:1587	a novel species	1573:1587	a novel species	1573:1587	Thus, strain KE2-3T should be recognised as a novel species of genus Jiangella, for which the name Jiangellaendophytica sp.
30556802	9	56	theme	strain	1535:1540	arg1	KE2-3T					1542:1547	strain KE2-3T	1535:1547	strain KE2-3T	1535:1547	Thus, strain KE2-3T should be recognised as a novel species of genus Jiangella, for which the name Jiangellaendophytica sp.
30556802	8	57	theme	closest	1510:1516	arg1	neighbour					1518:1526	its closest neighbour	1506:1526	its closest neighbour	1506:1526	With the differences of physiological, biochemical and genotypic data, strain KE2-3T could be discriminated from its closest neighbour.
30556802	2	58	theme	strain	150:155	arg1	KE2-3T					157:162	strain KE2-3T	150:162	strain KE2-3T	150:162	An endophytic actinobacterium, designated strain KE2-3T, was isolated from surface-sterilised rhizome of Kaempferia elegans.
30556802	6	59	theme	genome	1152:1157	arg1	sequence					1159:1166	the draft genome sequence	1142:1166	the draft genome sequence of strain KE2-3T	1142:1183	However, the draft genome sequence of strain KE2-3T exhibited low average nucleotide identity values to the reference strains (85.5-90.2 %), which were well below the 95-96 % species circumscription threshold.
30556802	5	60	theme	strain	820:825	arg1	member					854:859	a member	852:859	a member of the genus Jiangella	852:882	Based on 16S rRNA gene sequence analysis, strain KE2-3T was identified as a member of the genus Jiangella and showed the highest similarities to Jiangella muralis DSM 45357T (99.3 %) followed by Jiangella albaDSM 45237T (99.2 %), Jiangella alkaliphilia DSM 45079T (99.0 %), Jiangella gansuensisDSM 44835T (98.8 %) and Jiangella mangrovi3SM4-07T (98.6 %).
30556802	5	60	theme	strain	820:825	arg1	KE2-3T					827:832	strain KE2-3T	820:832	strain KE2-3T	820:832	Based on 16S rRNA gene sequence analysis, strain KE2-3T was identified as a member of the genus Jiangella and showed the highest similarities to Jiangella muralis DSM 45357T (99.3 %) followed by Jiangella albaDSM 45237T (99.2 %), Jiangella alkaliphilia DSM 45079T (99.0 %), Jiangella gansuensisDSM 44835T (98.8 %) and Jiangella mangrovi3SM4-07T (98.6 %).
30556802	5	60	theme	strain	820:825	arg1	similarities					907:918	the highest similarities	895:918	the highest similarities to Jiangella muralis DSM 45357T (99.3 %) followed by Jiangella albaDSM 45237T (99.2 %), Jiangella alkaliphilia DSM 45079T (99.0 %), Jiangella gansuensisDSM 44835T (98.8 %) and Jiangella mangrovi3SM4-07T (98.6 %)	895:1130	Based on 16S rRNA gene sequence analysis, strain KE2-3T was identified as a member of the genus Jiangella and showed the highest similarities to Jiangella muralis DSM 45357T (99.3 %) followed by Jiangella albaDSM 45237T (99.2 %), Jiangella alkaliphilia DSM 45079T (99.0 %), Jiangella gansuensisDSM 44835T (98.8 %) and Jiangella mangrovi3SM4-07T (98.6 %).
30556802	3	61	used	used	261:264	arg2	approach					248:255	The polyphasic approach	233:255	The polyphasic approach	233:255	The polyphasic approach was used for evaluating the taxonomic position of this strain.
30556802	4	62	theme	predominant	604:614	arg1	acids					622:626	the predominant fatty acids	600:626	the predominant fatty acids in cells	600:635	The taxonomic affiliation of this strain at genus level could be confirmed by its chemotaxonomic characteristic, i.e. the presence of ll-diaminopimelic acid in the cell peptidoglycan, MK-9(H4) as the major menaquinone, iso-C16 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0 as the predominant fatty acids in cells, and the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside in its membranes.
30556802	5	63	theme	gene	796:799	arg1	analysis					810:817	16S rRNA gene sequence analysis	787:817	16S rRNA gene sequence analysis	787:817	Based on 16S rRNA gene sequence analysis, strain KE2-3T was identified as a member of the genus Jiangella and showed the highest similarities to Jiangella muralis DSM 45357T (99.3 %) followed by Jiangella albaDSM 45237T (99.2 %), Jiangella alkaliphilia DSM 45079T (99.0 %), Jiangella gansuensisDSM 44835T (98.8 %) and Jiangella mangrovi3SM4-07T (98.6 %).
30556802	4	64	theme	ll-diaminopimelic	454:470	arg1	acid					472:475	ll-diaminopimelic acid	454:475	ll-diaminopimelic acid in the cell peptidoglycan	454:501	The taxonomic affiliation of this strain at genus level could be confirmed by its chemotaxonomic characteristic, i.e. the presence of ll-diaminopimelic acid in the cell peptidoglycan, MK-9(H4) as the major menaquinone, iso-C16 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0 as the predominant fatty acids in cells, and the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside in its membranes.
30556802	4	64	theme	ll-diaminopimelic	454:470	arg1	MK-9					504:507	MK-9	504:507	MK-9	504:507	The taxonomic affiliation of this strain at genus level could be confirmed by its chemotaxonomic characteristic, i.e. the presence of ll-diaminopimelic acid in the cell peptidoglycan, MK-9(H4) as the major menaquinone, iso-C16 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0 as the predominant fatty acids in cells, and the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside in its membranes.
30556802	4	65	from	level	370:374	arg1	affiliation					334:344	The taxonomic affiliation	320:344	The taxonomic affiliation of this strain at genus level	320:374	The taxonomic affiliation of this strain at genus level could be confirmed by its chemotaxonomic characteristic, i.e. the presence of ll-diaminopimelic acid in the cell peptidoglycan, MK-9(H4) as the major menaquinone, iso-C16 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0 as the predominant fatty acids in cells, and the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside in its membranes.
30556802	6	66	theme	nucleotide	1207:1216	arg1	identity					1218:1225	average nucleotide identity	1199:1225	low average nucleotide identity values	1195:1232	However, the draft genome sequence of strain KE2-3T exhibited low average nucleotide identity values to the reference strains (85.5-90.2 %), which were well below the 95-96 % species circumscription threshold.
30556802	5	67	theme	Jiangella	1008:1016	arg1	45079T					1035:1040	Jiangella alkaliphilia DSM 45079T	1008:1040	Jiangella alkaliphilia DSM 45079T (99.0 %)	1008:1049	Based on 16S rRNA gene sequence analysis, strain KE2-3T was identified as a member of the genus Jiangella and showed the highest similarities to Jiangella muralis DSM 45357T (99.3 %) followed by Jiangella albaDSM 45237T (99.2 %), Jiangella alkaliphilia DSM 45079T (99.0 %), Jiangella gansuensisDSM 44835T (98.8 %) and Jiangella mangrovi3SM4-07T (98.6 %).
30556802	5	67	theme	Jiangella	1008:1016	arg1	%					1048:1048	99.0 %	1043:1048	99.0 %	1043:1048	Based on 16S rRNA gene sequence analysis, strain KE2-3T was identified as a member of the genus Jiangella and showed the highest similarities to Jiangella muralis DSM 45357T (99.3 %) followed by Jiangella albaDSM 45237T (99.2 %), Jiangella alkaliphilia DSM 45079T (99.0 %), Jiangella gansuensisDSM 44835T (98.8 %) and Jiangella mangrovi3SM4-07T (98.6 %).
30556802	6	68	theme	low	1195:1197	arg1	values					1227:1232	low average nucleotide identity values	1195:1232	low average nucleotide identity values	1195:1232	However, the draft genome sequence of strain KE2-3T exhibited low average nucleotide identity values to the reference strains (85.5-90.2 %), which were well below the 95-96 % species circumscription threshold.
30556802	5	69	theme	Jiangella	874:882	arg1	member					854:859	a member	852:859	a member of the genus Jiangella	852:882	Based on 16S rRNA gene sequence analysis, strain KE2-3T was identified as a member of the genus Jiangella and showed the highest similarities to Jiangella muralis DSM 45357T (99.3 %) followed by Jiangella albaDSM 45237T (99.2 %), Jiangella alkaliphilia DSM 45079T (99.0 %), Jiangella gansuensisDSM 44835T (98.8 %) and Jiangella mangrovi3SM4-07T (98.6 %).
30556802	5	69	theme	Jiangella	874:882	arg1	KE2-3T					827:832	strain KE2-3T	820:832	strain KE2-3T	820:832	Based on 16S rRNA gene sequence analysis, strain KE2-3T was identified as a member of the genus Jiangella and showed the highest similarities to Jiangella muralis DSM 45357T (99.3 %) followed by Jiangella albaDSM 45237T (99.2 %), Jiangella alkaliphilia DSM 45079T (99.0 %), Jiangella gansuensisDSM 44835T (98.8 %) and Jiangella mangrovi3SM4-07T (98.6 %).
30556802	5	69	theme	Jiangella	874:882	arg1	similarities					907:918	the highest similarities	895:918	the highest similarities to Jiangella muralis DSM 45357T (99.3 %) followed by Jiangella albaDSM 45237T (99.2 %), Jiangella alkaliphilia DSM 45079T (99.0 %), Jiangella gansuensisDSM 44835T (98.8 %) and Jiangella mangrovi3SM4-07T (98.6 %)	895:1130	Based on 16S rRNA gene sequence analysis, strain KE2-3T was identified as a member of the genus Jiangella and showed the highest similarities to Jiangella muralis DSM 45357T (99.3 %) followed by Jiangella albaDSM 45237T (99.2 %), Jiangella alkaliphilia DSM 45079T (99.0 %), Jiangella gansuensisDSM 44835T (98.8 %) and Jiangella mangrovi3SM4-07T (98.6 %).
30556802	4	70	dep	characteristic	417:430	arg1	be					382:383	be	382:383	could be confirmed by its chemotaxonomic characteristic, i.e. the presence of ll-diaminopimelic acid in the cell peptidoglycan, MK-9(H4) as the major menaquinone, iso-C16 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0 as the predominant fatty acids in cells, and the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside in its membranes	376:775	The taxonomic affiliation of this strain at genus level could be confirmed by its chemotaxonomic characteristic, i.e. the presence of ll-diaminopimelic acid in the cell peptidoglycan, MK-9(H4) as the major menaquinone, iso-C16 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0 as the predominant fatty acids in cells, and the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside in its membranes.
30556802	9	71	theme	name	1623:1626	arg1	sp					1649:1650	the name Jiangellaendophytica sp	1619:1650	the name Jiangellaendophytica sp	1619:1650	Thus, strain KE2-3T should be recognised as a novel species of genus Jiangella, for which the name Jiangellaendophytica sp.
30556802	4	72	theme	cell	484:487	arg1	peptidoglycan					489:501	the cell peptidoglycan	480:501	the cell peptidoglycan	480:501	The taxonomic affiliation of this strain at genus level could be confirmed by its chemotaxonomic characteristic, i.e. the presence of ll-diaminopimelic acid in the cell peptidoglycan, MK-9(H4) as the major menaquinone, iso-C16 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0 as the predominant fatty acids in cells, and the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside in its membranes.
30556802	4	73	theme	phosphatidylinositol	704:723	arg1	presence					442:449	the presence	438:449	the presence of ll-diaminopimelic acid in the cell peptidoglycan, MK-9(H4) as the major menaquinone, iso-C16 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0 as the predominant fatty acids in cells,	438:636	The taxonomic affiliation of this strain at genus level could be confirmed by its chemotaxonomic characteristic, i.e. the presence of ll-diaminopimelic acid in the cell peptidoglycan, MK-9(H4) as the major menaquinone, iso-C16 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0 as the predominant fatty acids in cells, and the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside in its membranes.
30556802	4	73	theme	phosphatidylinositol	704:723	arg1	presence					646:653	the presence	642:653	the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside in its membranes	642:775	The taxonomic affiliation of this strain at genus level could be confirmed by its chemotaxonomic characteristic, i.e. the presence of ll-diaminopimelic acid in the cell peptidoglycan, MK-9(H4) as the major menaquinone, iso-C16 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0 as the predominant fatty acids in cells, and the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside in its membranes.
30556802	5	74	theme	DSM	1031:1033	arg1	45079T					1035:1040	Jiangella alkaliphilia DSM 45079T	1008:1040	Jiangella alkaliphilia DSM 45079T (99.0 %)	1008:1049	Based on 16S rRNA gene sequence analysis, strain KE2-3T was identified as a member of the genus Jiangella and showed the highest similarities to Jiangella muralis DSM 45357T (99.3 %) followed by Jiangella albaDSM 45237T (99.2 %), Jiangella alkaliphilia DSM 45079T (99.0 %), Jiangella gansuensisDSM 44835T (98.8 %) and Jiangella mangrovi3SM4-07T (98.6 %).
30556802	5	74	theme	DSM	1031:1033	arg1	%					1048:1048	99.0 %	1043:1048	99.0 %	1043:1048	Based on 16S rRNA gene sequence analysis, strain KE2-3T was identified as a member of the genus Jiangella and showed the highest similarities to Jiangella muralis DSM 45357T (99.3 %) followed by Jiangella albaDSM 45237T (99.2 %), Jiangella alkaliphilia DSM 45079T (99.0 %), Jiangella gansuensisDSM 44835T (98.8 %) and Jiangella mangrovi3SM4-07T (98.6 %).
30556802	5	75	theme	muralis	933:939	arg1	%					958:958	99.3 %	953:958	99.3 %	953:958	Based on 16S rRNA gene sequence analysis, strain KE2-3T was identified as a member of the genus Jiangella and showed the highest similarities to Jiangella muralis DSM 45357T (99.3 %) followed by Jiangella albaDSM 45237T (99.2 %), Jiangella alkaliphilia DSM 45079T (99.0 %), Jiangella gansuensisDSM 44835T (98.8 %) and Jiangella mangrovi3SM4-07T (98.6 %).
30556802	5	75	theme	muralis	933:939	arg1	DSM					941:943	Jiangella muralis DSM 45357T	923:950	Jiangella muralis DSM 45357T (99.3 %) followed by Jiangella albaDSM 45237T (99.2 %), Jiangella alkaliphilia DSM 45079T (99.0 %), Jiangella gansuensisDSM 44835T (98.8 %) and Jiangella mangrovi3SM4-07T (98.6 %)	923:1130	Based on 16S rRNA gene sequence analysis, strain KE2-3T was identified as a member of the genus Jiangella and showed the highest similarities to Jiangella muralis DSM 45357T (99.3 %) followed by Jiangella albaDSM 45237T (99.2 %), Jiangella alkaliphilia DSM 45079T (99.0 %), Jiangella gansuensisDSM 44835T (98.8 %) and Jiangella mangrovi3SM4-07T (98.6 %).
30556802	4	76	theme	phosphatidylinositol	729:748	arg1	mannoside					750:758	phosphatidylinositol mannoside	729:758	phosphatidylinositol mannoside	729:758	The taxonomic affiliation of this strain at genus level could be confirmed by its chemotaxonomic characteristic, i.e. the presence of ll-diaminopimelic acid in the cell peptidoglycan, MK-9(H4) as the major menaquinone, iso-C16 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0 as the predominant fatty acids in cells, and the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside in its membranes.
30556802	0	77	theme	endophytica	10:20	arg1	sp					22:23	Jiangella endophytica sp	0:23	Jiangella endophytica sp.	0:24	Jiangella endophytica sp.
30556802	4	78	theme	diphosphatidylglycerol	658:679	arg1	presence					442:449	the presence	438:449	the presence of ll-diaminopimelic acid in the cell peptidoglycan, MK-9(H4) as the major menaquinone, iso-C16 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0 as the predominant fatty acids in cells,	438:636	The taxonomic affiliation of this strain at genus level could be confirmed by its chemotaxonomic characteristic, i.e. the presence of ll-diaminopimelic acid in the cell peptidoglycan, MK-9(H4) as the major menaquinone, iso-C16 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0 as the predominant fatty acids in cells, and the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside in its membranes.
30556802	4	78	theme	diphosphatidylglycerol	658:679	arg1	presence					646:653	the presence	642:653	the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside in its membranes	642:775	The taxonomic affiliation of this strain at genus level could be confirmed by its chemotaxonomic characteristic, i.e. the presence of ll-diaminopimelic acid in the cell peptidoglycan, MK-9(H4) as the major menaquinone, iso-C16 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0 as the predominant fatty acids in cells, and the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside in its membranes.
30556802	5	79	theme	16S	787:789	arg1	analysis					810:817	16S rRNA gene sequence analysis	787:817	16S rRNA gene sequence analysis	787:817	Based on 16S rRNA gene sequence analysis, strain KE2-3T was identified as a member of the genus Jiangella and showed the highest similarities to Jiangella muralis DSM 45357T (99.3 %) followed by Jiangella albaDSM 45237T (99.2 %), Jiangella alkaliphilia DSM 45079T (99.0 %), Jiangella gansuensisDSM 44835T (98.8 %) and Jiangella mangrovi3SM4-07T (98.6 %).
30556802	1	80	theme	Kaempferia	88:97	arg1	elegans					99:105	Kaempferia elegans	88:105	Kaempferia elegans	88:105	nov., an endophytic actinomycete isolated from the rhizome of Kaempferia elegans.
30556802	2	81	attach	isolated	169:176	arg1	rhizome					202:208	surface-sterilised rhizome	183:208	surface-sterilised rhizome of Kaempferia elegans	183:230	An endophytic actinobacterium, designated strain KE2-3T, was isolated from surface-sterilised rhizome of Kaempferia elegans.
30556802	2	81	attach	isolated	169:176	arg2	actinobacterium					122:136	An endophytic actinobacterium	108:136	An endophytic actinobacterium	108:136	An endophytic actinobacterium, designated strain KE2-3T, was isolated from surface-sterilised rhizome of Kaempferia elegans.
30556802	4	82	theme	phosphatidylglycerol	682:701	arg1	presence					442:449	the presence	438:449	the presence of ll-diaminopimelic acid in the cell peptidoglycan, MK-9(H4) as the major menaquinone, iso-C16 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0 as the predominant fatty acids in cells,	438:636	The taxonomic affiliation of this strain at genus level could be confirmed by its chemotaxonomic characteristic, i.e. the presence of ll-diaminopimelic acid in the cell peptidoglycan, MK-9(H4) as the major menaquinone, iso-C16 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0 as the predominant fatty acids in cells, and the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside in its membranes.
30556802	4	82	theme	phosphatidylglycerol	682:701	arg1	presence					646:653	the presence	642:653	the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside in its membranes	642:775	The taxonomic affiliation of this strain at genus level could be confirmed by its chemotaxonomic characteristic, i.e. the presence of ll-diaminopimelic acid in the cell peptidoglycan, MK-9(H4) as the major menaquinone, iso-C16 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0 as the predominant fatty acids in cells, and the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside in its membranes.
30556802	8	83	theme	data	1458:1461	arg1	differences					1402:1412	the differences	1398:1412	the differences of physiological, biochemical and genotypic data	1398:1461	With the differences of physiological, biochemical and genotypic data, strain KE2-3T could be discriminated from its closest neighbour.
30556802	4	84	attach	presence	442:449	arg1	membranes					767:775	its membranes	763:775	its membranes	763:775	The taxonomic affiliation of this strain at genus level could be confirmed by its chemotaxonomic characteristic, i.e. the presence of ll-diaminopimelic acid in the cell peptidoglycan, MK-9(H4) as the major menaquinone, iso-C16 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0 as the predominant fatty acids in cells, and the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside in its membranes.
30556802	4	84	attach	presence	442:449	arg2	diphosphatidylglycerol					658:679	diphosphatidylglycerol	658:679	diphosphatidylglycerol	658:679	The taxonomic affiliation of this strain at genus level could be confirmed by its chemotaxonomic characteristic, i.e. the presence of ll-diaminopimelic acid in the cell peptidoglycan, MK-9(H4) as the major menaquinone, iso-C16 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0 as the predominant fatty acids in cells, and the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside in its membranes.
30556802	4	84	attach	presence	442:449	arg2	acid					472:475	ll-diaminopimelic acid	454:475	ll-diaminopimelic acid in the cell peptidoglycan	454:501	The taxonomic affiliation of this strain at genus level could be confirmed by its chemotaxonomic characteristic, i.e. the presence of ll-diaminopimelic acid in the cell peptidoglycan, MK-9(H4) as the major menaquinone, iso-C16 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0 as the predominant fatty acids in cells, and the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside in its membranes.
30556802	4	84	attach	presence	442:449	arg2	MK-9					504:507	MK-9	504:507	MK-9	504:507	The taxonomic affiliation of this strain at genus level could be confirmed by its chemotaxonomic characteristic, i.e. the presence of ll-diaminopimelic acid in the cell peptidoglycan, MK-9(H4) as the major menaquinone, iso-C16 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0 as the predominant fatty acids in cells, and the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside in its membranes.
30556802	4	84	attach	presence	442:449	arg2	phosphatidylglycerol					682:701	phosphatidylglycerol	682:701	phosphatidylglycerol	682:701	The taxonomic affiliation of this strain at genus level could be confirmed by its chemotaxonomic characteristic, i.e. the presence of ll-diaminopimelic acid in the cell peptidoglycan, MK-9(H4) as the major menaquinone, iso-C16 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0 as the predominant fatty acids in cells, and the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside in its membranes.
30556802	4	84	attach	presence	442:449	arg2	phosphatidylinositol					704:723	phosphatidylinositol	704:723	phosphatidylinositol	704:723	The taxonomic affiliation of this strain at genus level could be confirmed by its chemotaxonomic characteristic, i.e. the presence of ll-diaminopimelic acid in the cell peptidoglycan, MK-9(H4) as the major menaquinone, iso-C16 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0 as the predominant fatty acids in cells, and the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside in its membranes.
30556802	4	84	attach	presence	442:449	arg2	mannoside					750:758	phosphatidylinositol mannoside	729:758	phosphatidylinositol mannoside	729:758	The taxonomic affiliation of this strain at genus level could be confirmed by its chemotaxonomic characteristic, i.e. the presence of ll-diaminopimelic acid in the cell peptidoglycan, MK-9(H4) as the major menaquinone, iso-C16 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0 as the predominant fatty acids in cells, and the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside in its membranes.
30556802	5	85	theme	gansuensisDSM	1062:1074	arg1	44835T					1076:1081	Jiangella gansuensisDSM 44835T	1052:1081	Jiangella gansuensisDSM 44835T (98.8 %)	1052:1090	Based on 16S rRNA gene sequence analysis, strain KE2-3T was identified as a member of the genus Jiangella and showed the highest similarities to Jiangella muralis DSM 45357T (99.3 %) followed by Jiangella albaDSM 45237T (99.2 %), Jiangella alkaliphilia DSM 45079T (99.0 %), Jiangella gansuensisDSM 44835T (98.8 %) and Jiangella mangrovi3SM4-07T (98.6 %).
30556802	5	85	theme	gansuensisDSM	1062:1074	arg1	%					1089:1089	98.8 %	1084:1089	98.8 %	1084:1089	Based on 16S rRNA gene sequence analysis, strain KE2-3T was identified as a member of the genus Jiangella and showed the highest similarities to Jiangella muralis DSM 45357T (99.3 %) followed by Jiangella albaDSM 45237T (99.2 %), Jiangella alkaliphilia DSM 45079T (99.0 %), Jiangella gansuensisDSM 44835T (98.8 %) and Jiangella mangrovi3SM4-07T (98.6 %).
30556802	8	86	theme	strain	1464:1469	arg1	KE2-3T					1471:1476	strain KE2-3T	1464:1476	strain KE2-3T	1464:1476	With the differences of physiological, biochemical and genotypic data, strain KE2-3T could be discriminated from its closest neighbour.
30556802	2	87	theme	Kaempferia	213:222	arg1	elegans					224:230	Kaempferia elegans	213:230	Kaempferia elegans	213:230	An endophytic actinobacterium, designated strain KE2-3T, was isolated from surface-sterilised rhizome of Kaempferia elegans.
30556802	4	88	dep	MK-9	504:507	arg1	 0					594:595	 0	594:595	 0	594:595	The taxonomic affiliation of this strain at genus level could be confirmed by its chemotaxonomic characteristic, i.e. the presence of ll-diaminopimelic acid in the cell peptidoglycan, MK-9(H4) as the major menaquinone, iso-C16 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0 as the predominant fatty acids in cells, and the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside in its membranes.
30556802	4	88	dep	MK-9	504:507	arg1	iso-C15 					585:592	iso-C15 	585:592	iso-C15 	585:592	The taxonomic affiliation of this strain at genus level could be confirmed by its chemotaxonomic characteristic, i.e. the presence of ll-diaminopimelic acid in the cell peptidoglycan, MK-9(H4) as the major menaquinone, iso-C16 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0 as the predominant fatty acids in cells, and the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside in its membranes.
30556802	4	88	dep	MK-9	504:507	arg1	 0					578:579	 0	578:579	 0	578:579	The taxonomic affiliation of this strain at genus level could be confirmed by its chemotaxonomic characteristic, i.e. the presence of ll-diaminopimelic acid in the cell peptidoglycan, MK-9(H4) as the major menaquinone, iso-C16 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0 as the predominant fatty acids in cells, and the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside in its membranes.
30556802	11	89	theme	=BCC	1698:1701	arg1	110004T					1715:1721	=BCC 66359T=NBRC 110004T	1698:1721	=BCC 66359T=NBRC 110004T	1698:1721	The type strain is KE2-3T (=BCC 66359T=NBRC 110004T).
30556802	11	89	theme	=BCC	1698:1701	arg1	KE2-3T					1690:1695	KE2-3T	1690:1695	KE2-3T (=BCC 66359T=NBRC 110004T)	1690:1722	The type strain is KE2-3T (=BCC 66359T=NBRC 110004T).
30556802	7	90	theme	72.3 mol	1382:1389	arg1	%					1390:1390	72.3 mol%	1382:1390	72.3 mol%	1382:1390	The DNA G+C content of genomic DNA was 72.3 mol%.
30556802	7	90	theme	72.3 mol	1382:1389	arg1	G+C content					1351:1361	The DNA G+C content	1343:1361	The DNA G+C content of genomic DNA	1343:1376	The DNA G+C content of genomic DNA was 72.3 mol%.
30556802	6	91	theme	circumscription	1316:1330	arg1	threshold					1332:1340	the 95-96 % species circumscription threshold	1296:1340	the 95-96 % species circumscription threshold	1296:1340	However, the draft genome sequence of strain KE2-3T exhibited low average nucleotide identity values to the reference strains (85.5-90.2 %), which were well below the 95-96 % species circumscription threshold.
30230442	11	0	theme	sacelli	1372:1378	arg1	value					1308:1312	The average nucleotide identity value	1276:1312	The average nucleotide identity value between strain M1HQ-2T and type strain of Brachybacterium sacelli	1276:1378	The average nucleotide identity value between strain M1HQ-2T and type strain of Brachybacterium sacelli was 76.7 %.
30230442	11	0	theme	sacelli	1372:1378	arg1	%					1389:1389	76.7 %	1384:1389	76.7 %	1384:1389	The average nucleotide identity value between strain M1HQ-2T and type strain of Brachybacterium sacelli was 76.7 %.
30230442	13	1	theme	strain	1590:1595	arg1	M1HQ-2T					1597:1603	strain M1HQ-2T	1590:1603	strain M1HQ-2T	1590:1603	On the basis of phylogenetic analysis, chemotaxonomic characteristics and phenotypic data, strain M1HQ-2T represents a novel species of the genus Brachybacterium, for which the name Brachybacteriumendophyticum sp.
30230442	12	2	theme	DNA-DNA	1406:1412	arg1	hybridization					1414:1426	DNA-DNA hybridization	1406:1426	The estimated DNA-DNA hybridization value between strain M1HQ-2T and type strain of B. sacelli	1392:1485	The estimated DNA-DNA hybridization value between strain M1HQ-2T and type strain of B. sacelli was 20.6 %.
30230442	12	3	theme	estimated	1396:1404	arg1	value					1428:1432	The estimated DNA-DNA hybridization value	1392:1432	The estimated DNA-DNA hybridization value between strain M1HQ-2T and type strain of B. sacelli	1392:1485	The estimated DNA-DNA hybridization value between strain M1HQ-2T and type strain of B. sacelli was 20.6 %.
30230442	12	3	theme	estimated	1396:1404	arg1	%					1496:1496	20.6 %	1491:1496	20.6 %	1491:1496	The estimated DNA-DNA hybridization value between strain M1HQ-2T and type strain of B. sacelli was 20.6 %.
30230442	2	4	theme	baicalensis	290:300	arg1	Georgi					302:307	Scutellaria baicalensis Georgi	278:307	Scutellaria baicalensis Georgi collected from Guizhou, China	278:337	A Gram-positive, aerobic, coccus-shaped, non-spore-forming actinobacterium, designated strain M1HQ-2T, was isolated from a surface-sterilized bark of Scutellaria baicalensis Georgi collected from Guizhou, China and tested using a polyphasic approach to determine its taxonomic position.
30230442	5	5	theme	16S	794:796	arg1	similarity					817:826	the highest 16S rRNA gene sequence similarity	782:826	the highest 16S rRNA gene sequence similarity of 97.6 % to Brachybacteriumsquillarum M-6-3T	782:872	Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain M1HQ-2T belonged to the genus Brachybacterium and had the highest 16S rRNA gene sequence similarity of 97.6 % to Brachybacteriumsquillarum M-6-3T.
30230442	11	6	theme	average	1280:1286	arg1	value					1308:1312	The average nucleotide identity value	1276:1312	The average nucleotide identity value between strain M1HQ-2T and type strain of Brachybacterium sacelli	1276:1378	The average nucleotide identity value between strain M1HQ-2T and type strain of Brachybacterium sacelli was 76.7 %.
30230442	11	6	theme	average	1280:1286	arg1	%					1389:1389	76.7 %	1384:1389	76.7 %	1384:1389	The average nucleotide identity value between strain M1HQ-2T and type strain of Brachybacterium sacelli was 76.7 %.
30230442	5	7	theme	gene	803:806	arg1	similarity					817:826	the highest 16S rRNA gene sequence similarity	782:826	the highest 16S rRNA gene sequence similarity of 97.6 % to Brachybacteriumsquillarum M-6-3T	782:872	Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain M1HQ-2T belonged to the genus Brachybacterium and had the highest 16S rRNA gene sequence similarity of 97.6 % to Brachybacteriumsquillarum M-6-3T.
30230442	3	8	theme	0-15 	508:512	arg1	NaCl					521:524	0-15 % (w/v) NaCl	508:524	0-15 % (w/v) NaCl (1-3 %)	508:532	Strain M1HQ-2T grew at 4-37 °C (optimum, 30 °C), pH 5.0-11.0 (pH 8.0) and in the presence of 0-15 % (w/v) NaCl (1-3 %).
30230442	3	8	theme	0-15 	508:512	arg1	%					531:531	1-3 %	527:531	1-3 %	527:531	Strain M1HQ-2T grew at 4-37 °C (optimum, 30 °C), pH 5.0-11.0 (pH 8.0) and in the presence of 0-15 % (w/v) NaCl (1-3 %).
30230442	7	9	theme	meso-diaminopimelic	972:990	arg1	acid					992:995	meso-diaminopimelic acid	972:995	meso-diaminopimelic acid	972:995	The cell-wall peptidoglycan contained meso-diaminopimelic acid.
30230442	11	10	theme	type	1341:1344	arg1	strain					1346:1351	type strain	1341:1351	type strain	1341:1351	The average nucleotide identity value between strain M1HQ-2T and type strain of Brachybacterium sacelli was 76.7 %.
30230442	13	11	theme	chemotaxonomic	1538:1551	arg1	characteristics					1553:1567	chemotaxonomic characteristics	1538:1567	chemotaxonomic characteristics	1538:1567	On the basis of phylogenetic analysis, chemotaxonomic characteristics and phenotypic data, strain M1HQ-2T represents a novel species of the genus Brachybacterium, for which the name Brachybacteriumendophyticum sp.
30230442	9	12	dep	anteiso-C15 	1186:1197	arg1	 0					1219:1220	 0	1219:1220	anteiso-C15 : 0 and anteiso-C17 : 0	1186:1220	The predominant fatty acids were anteiso-C15 : 0 and anteiso-C17 : 0.
30230442	9	12	dep	anteiso-C15 	1186:1197	arg1	anteiso-C17 					1206:1217	anteiso-C17 	1206:1217	anteiso-C17 	1206:1217	The predominant fatty acids were anteiso-C15 : 0 and anteiso-C17 : 0.
30230442	9	12	dep	anteiso-C15 	1186:1197	arg1	 0					1199:1200	 0	1199:1200	 0	1199:1200	The predominant fatty acids were anteiso-C15 : 0 and anteiso-C17 : 0.
30230442	5	13	theme	rRNA	687:690	arg1	sequence					697:704	16S rRNA gene sequence	683:704	16S rRNA gene sequence	683:704	Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain M1HQ-2T belonged to the genus Brachybacterium and had the highest 16S rRNA gene sequence similarity of 97.6 % to Brachybacteriumsquillarum M-6-3T.
30230442	2	14	theme	surface-sterilized	251:268	arg1	bark					270:273	a surface-sterilized bark	249:273	a surface-sterilized bark of Scutellaria baicalensis Georgi collected from Guizhou, China	249:337	A Gram-positive, aerobic, coccus-shaped, non-spore-forming actinobacterium, designated strain M1HQ-2T, was isolated from a surface-sterilized bark of Scutellaria baicalensis Georgi collected from Guizhou, China and tested using a polyphasic approach to determine its taxonomic position.
30230442	13	15	theme	Brachybacteriumendophyticum	1681:1707	arg1	sp					1709:1710	the name Brachybacteriumendophyticum sp	1672:1710	the name Brachybacteriumendophyticum sp	1672:1710	On the basis of phylogenetic analysis, chemotaxonomic characteristics and phenotypic data, strain M1HQ-2T represents a novel species of the genus Brachybacterium, for which the name Brachybacteriumendophyticum sp.
30230442	10	16	theme	strain	1246:1251	arg1	M1HQ-2T					1253:1259	strain M1HQ-2T	1246:1259	strain M1HQ-2T	1246:1259	The DNA G+C content of strain M1HQ-2T was 71.0 mol%.
30230442	8	17	theme	polar	1002:1006	arg1	profile					1015:1021	The polar lipids profile	998:1021	The polar lipids profile of strain M1HQ-2T	998:1039	The polar lipids profile of strain M1HQ-2T contained diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid and an unidentified lipid.
30230442	2	18	attach	isolated	235:242	arg1	bark					270:273	a surface-sterilized bark	249:273	a surface-sterilized bark of Scutellaria baicalensis Georgi collected from Guizhou, China	249:337	A Gram-positive, aerobic, coccus-shaped, non-spore-forming actinobacterium, designated strain M1HQ-2T, was isolated from a surface-sterilized bark of Scutellaria baicalensis Georgi collected from Guizhou, China and tested using a polyphasic approach to determine its taxonomic position.
30230442	2	18	attach	isolated	235:242	arg2	actinobacterium					187:201	A Gram-positive, aerobic, coccus-shaped, non-spore-forming actinobacterium	128:201	A Gram-positive, aerobic, coccus-shaped, non-spore-forming actinobacterium	128:201	A Gram-positive, aerobic, coccus-shaped, non-spore-forming actinobacterium, designated strain M1HQ-2T, was isolated from a surface-sterilized bark of Scutellaria baicalensis Georgi collected from Guizhou, China and tested using a polyphasic approach to determine its taxonomic position.
30230442	11	19	theme	nucleotide	1288:1297	arg1	value					1308:1312	The average nucleotide identity value	1276:1312	The average nucleotide identity value between strain M1HQ-2T and type strain of Brachybacterium sacelli	1276:1378	The average nucleotide identity value between strain M1HQ-2T and type strain of Brachybacterium sacelli was 76.7 %.
30230442	11	19	theme	nucleotide	1288:1297	arg1	%					1389:1389	76.7 %	1384:1389	76.7 %	1384:1389	The average nucleotide identity value between strain M1HQ-2T and type strain of Brachybacterium sacelli was 76.7 %.
30230442	5	20	contain	had	778:780	arg1	M1HQ-2T					728:734	strain M1HQ-2T	721:734	strain M1HQ-2T	721:734	Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain M1HQ-2T belonged to the genus Brachybacterium and had the highest 16S rRNA gene sequence similarity of 97.6 % to Brachybacteriumsquillarum M-6-3T.
30230442	5	20	contain	had	778:780	arg2	similarity					817:826	the highest 16S rRNA gene sequence similarity	782:826	the highest 16S rRNA gene sequence similarity of 97.6 % to Brachybacteriumsquillarum M-6-3T	782:872	Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain M1HQ-2T belonged to the genus Brachybacterium and had the highest 16S rRNA gene sequence similarity of 97.6 % to Brachybacteriumsquillarum M-6-3T.
30230442	0	21	theme	Brachybacterium	0:14	arg1	sp					29:30	Brachybacterium endophyticum sp	0:30	Brachybacterium endophyticum sp.	0:31	Brachybacterium endophyticum sp.
30230442	8	22	theme	strain	1026:1031	arg1	M1HQ-2T					1033:1039	strain M1HQ-2T	1026:1039	strain M1HQ-2T	1026:1039	The polar lipids profile of strain M1HQ-2T contained diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid and an unidentified lipid.
30230442	13	23	dep	analysis	1528:1535	arg1	basis					1506:1510	basis	1506:1510	basis	1506:1510	On the basis of phylogenetic analysis, chemotaxonomic characteristics and phenotypic data, strain M1HQ-2T represents a novel species of the genus Brachybacterium, for which the name Brachybacteriumendophyticum sp.
30230442	13	23	dep	analysis	1528:1535	arg1	the					1502:1504	the	1502:1504	the	1502:1504	On the basis of phylogenetic analysis, chemotaxonomic characteristics and phenotypic data, strain M1HQ-2T represents a novel species of the genus Brachybacterium, for which the name Brachybacteriumendophyticum sp.
30230442	5	24	theme	genus	752:756	arg1	Brachybacterium					758:772	the genus Brachybacterium	748:772	the genus Brachybacterium	748:772	Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain M1HQ-2T belonged to the genus Brachybacterium and had the highest 16S rRNA gene sequence similarity of 97.6 % to Brachybacteriumsquillarum M-6-3T.
30230442	1	25	theme	baicalensis	108:118	arg1	Georgi					120:125	Scutellaria baicalensis Georgi	96:125	Scutellaria baicalensis Georgi	96:125	nov., a novel endophytic actinobacterium isolated from bark of Scutellaria baicalensis Georgi.
30230442	13	26	theme	Brachybacterium	1645:1659	arg1	species					1624:1630	a novel species	1616:1630	a novel species	1616:1630	On the basis of phylogenetic analysis, chemotaxonomic characteristics and phenotypic data, strain M1HQ-2T represents a novel species of the genus Brachybacterium, for which the name Brachybacteriumendophyticum sp.
30230442	15	27	theme	=KCTC	1759:1763	arg1	1.16391T					1778:1785	=KCTC 49087T=CGMCC 1.16391T	1759:1785	=KCTC 49087T=CGMCC 1.16391T	1759:1785	The type strain is M1HQ-2T (=KCTC 49087T=CGMCC 1.16391T).
30230442	15	27	theme	=KCTC	1759:1763	arg1	M1HQ-2T					1750:1756	M1HQ-2T	1750:1756	M1HQ-2T (=KCTC 49087T=CGMCC 1.16391T)	1750:1786	The type strain is M1HQ-2T (=KCTC 49087T=CGMCC 1.16391T).
30230442	3	28	dep	optimum	447:453	arg1	30 °C					456:460	30 °C	456:460	30 °C	456:460	Strain M1HQ-2T grew at 4-37 °C (optimum, 30 °C), pH 5.0-11.0 (pH 8.0) and in the presence of 0-15 % (w/v) NaCl (1-3 %).
30230442	2	29	theme	polyphasic	358:367	arg1	approach					369:376	a polyphasic approach	356:376	a polyphasic approach to determine its taxonomic position	356:412	A Gram-positive, aerobic, coccus-shaped, non-spore-forming actinobacterium, designated strain M1HQ-2T, was isolated from a surface-sterilized bark of Scutellaria baicalensis Georgi collected from Guizhou, China and tested using a polyphasic approach to determine its taxonomic position.
30230442	1	30	theme	Georgi	120:125	arg1	bark					88:91	bark	88:91	bark of Scutellaria baicalensis Georgi	88:125	nov., a novel endophytic actinobacterium isolated from bark of Scutellaria baicalensis Georgi.
30230442	8	31	theme	unidentified	1100:1111	arg1	phospholipid					1113:1124	an unidentified phospholipid	1097:1124	an unidentified phospholipid	1097:1124	The polar lipids profile of strain M1HQ-2T contained diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid and an unidentified lipid.
30230442	12	32	theme	sacelli	1479:1485	arg1	value					1428:1432	The estimated DNA-DNA hybridization value	1392:1432	The estimated DNA-DNA hybridization value between strain M1HQ-2T and type strain of B. sacelli	1392:1485	The estimated DNA-DNA hybridization value between strain M1HQ-2T and type strain of B. sacelli was 20.6 %.
30230442	12	32	theme	sacelli	1479:1485	arg1	%					1496:1496	20.6 %	1491:1496	20.6 %	1491:1496	The estimated DNA-DNA hybridization value between strain M1HQ-2T and type strain of B. sacelli was 20.6 %.
30230442	4	33	theme	Substrate	535:543	arg1	mycelia					545:551	Substrate mycelia	535:551	Substrate mycelia	535:551	Substrate mycelia and aerial mycelia were not formed, and diffusible pigments were not observed on any media tested.
30230442	1	34	theme	novel	41:45	arg1	actinobacterium					58:72	a novel endophytic actinobacterium	39:72	a novel endophytic actinobacterium isolated from bark of Scutellaria baicalensis Georgi	39:125	nov., a novel endophytic actinobacterium isolated from bark of Scutellaria baicalensis Georgi.
30230442	11	35	theme	Brachybacterium	1356:1370	arg1	sacelli					1372:1378	Brachybacterium sacelli	1356:1378	Brachybacterium sacelli	1356:1378	The average nucleotide identity value between strain M1HQ-2T and type strain of Brachybacterium sacelli was 76.7 %.
30230442	9	36	theme	fatty	1169:1173	arg1	anteiso-C15 					1186:1197	anteiso-C15 	1186:1197	anteiso-C15 	1186:1197	The predominant fatty acids were anteiso-C15 : 0 and anteiso-C17 : 0.
30230442	9	36	theme	fatty	1169:1173	arg1	acids					1175:1179	The predominant fatty acids	1153:1179	The predominant fatty acids	1153:1179	The predominant fatty acids were anteiso-C15 : 0 and anteiso-C17 : 0.
30230442	8	37	contain	contained	1041:1049	arg2	phospholipid					1113:1124	an unidentified phospholipid	1097:1124	an unidentified phospholipid	1097:1124	The polar lipids profile of strain M1HQ-2T contained diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid and an unidentified lipid.
30230442	8	37	contain	contained	1041:1049	arg2	phosphatidylglycerol					1075:1094	phosphatidylglycerol	1075:1094	phosphatidylglycerol	1075:1094	The polar lipids profile of strain M1HQ-2T contained diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid and an unidentified lipid.
30230442	8	37	contain	contained	1041:1049	arg2	lipid					1146:1150	an unidentified lipid	1130:1150	an unidentified lipid	1130:1150	The polar lipids profile of strain M1HQ-2T contained diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid and an unidentified lipid.
30230442	8	37	contain	contained	1041:1049	arg2	diphosphatidylglycerol					1051:1072	diphosphatidylglycerol	1051:1072	diphosphatidylglycerol	1051:1072	The polar lipids profile of strain M1HQ-2T contained diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid and an unidentified lipid.
30230442	8	37	contain	contained	1041:1049	arg1	profile					1015:1021	The polar lipids profile	998:1021	The polar lipids profile of strain M1HQ-2T	998:1039	The polar lipids profile of strain M1HQ-2T contained diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid and an unidentified lipid.
30230442	8	38	theme	unidentified	1133:1144	arg1	lipid					1146:1150	an unidentified lipid	1130:1150	an unidentified lipid	1130:1150	The polar lipids profile of strain M1HQ-2T contained diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid and an unidentified lipid.
30230442	12	39	theme	type	1461:1464	arg1	strain					1466:1471	type strain	1461:1471	type strain	1461:1471	The estimated DNA-DNA hybridization value between strain M1HQ-2T and type strain of B. sacelli was 20.6 %.
30230442	7	40	contain	contained	962:970	arg2	acid					992:995	meso-diaminopimelic acid	972:995	meso-diaminopimelic acid	972:995	The cell-wall peptidoglycan contained meso-diaminopimelic acid.
30230442	7	40	contain	contained	962:970	arg1	peptidoglycan					948:960	The cell-wall peptidoglycan	934:960	The cell-wall peptidoglycan	934:960	The cell-wall peptidoglycan contained meso-diaminopimelic acid.
30230442	3	41	theme	%	513:513	arg1	NaCl					521:524	0-15 % (w/v) NaCl	508:524	0-15 % (w/v) NaCl (1-3 %)	508:532	Strain M1HQ-2T grew at 4-37 °C (optimum, 30 °C), pH 5.0-11.0 (pH 8.0) and in the presence of 0-15 % (w/v) NaCl (1-3 %).
30230442	3	41	theme	%	513:513	arg1	%					531:531	1-3 %	527:531	1-3 %	527:531	Strain M1HQ-2T grew at 4-37 °C (optimum, 30 °C), pH 5.0-11.0 (pH 8.0) and in the presence of 0-15 % (w/v) NaCl (1-3 %).
30230442	12	42	theme	hybridization	1414:1426	arg1	value					1428:1432	The estimated DNA-DNA hybridization value	1392:1432	The estimated DNA-DNA hybridization value between strain M1HQ-2T and type strain of B. sacelli	1392:1485	The estimated DNA-DNA hybridization value between strain M1HQ-2T and type strain of B. sacelli was 20.6 %.
30230442	12	42	theme	hybridization	1414:1426	arg1	%					1496:1496	20.6 %	1491:1496	20.6 %	1491:1496	The estimated DNA-DNA hybridization value between strain M1HQ-2T and type strain of B. sacelli was 20.6 %.
30230442	2	43	theme	Georgi	302:307	arg1	bark					270:273	a surface-sterilized bark	249:273	a surface-sterilized bark of Scutellaria baicalensis Georgi collected from Guizhou, China	249:337	A Gram-positive, aerobic, coccus-shaped, non-spore-forming actinobacterium, designated strain M1HQ-2T, was isolated from a surface-sterilized bark of Scutellaria baicalensis Georgi collected from Guizhou, China and tested using a polyphasic approach to determine its taxonomic position.
30230442	5	44	theme	highest	786:792	arg1	similarity					817:826	the highest 16S rRNA gene sequence similarity	782:826	the highest 16S rRNA gene sequence similarity of 97.6 % to Brachybacteriumsquillarum M-6-3T	782:872	Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain M1HQ-2T belonged to the genus Brachybacterium and had the highest 16S rRNA gene sequence similarity of 97.6 % to Brachybacteriumsquillarum M-6-3T.
30230442	11	45	theme	strain	1322:1327	arg1	M1HQ-2T					1329:1335	strain M1HQ-2T	1322:1335	strain M1HQ-2T	1322:1335	The average nucleotide identity value between strain M1HQ-2T and type strain of Brachybacterium sacelli was 76.7 %.
30230442	3	46	theme	NaCl	521:524	arg1	presence					496:503	the presence	492:503	the presence of 0-15 % (w/v) NaCl (1-3 %)	492:532	Strain M1HQ-2T grew at 4-37 °C (optimum, 30 °C), pH 5.0-11.0 (pH 8.0) and in the presence of 0-15 % (w/v) NaCl (1-3 %).
30230442	5	47	theme	rRNA	798:801	arg1	similarity					817:826	the highest 16S rRNA gene sequence similarity	782:826	the highest 16S rRNA gene sequence similarity of 97.6 % to Brachybacteriumsquillarum M-6-3T	782:872	Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain M1HQ-2T belonged to the genus Brachybacterium and had the highest 16S rRNA gene sequence similarity of 97.6 % to Brachybacteriumsquillarum M-6-3T.
30230442	6	48	theme	dominant	912:919	arg1	menaquinone					921:931	the dominant menaquinone	908:931	the dominant menaquinone	908:931	Strain M1HQ-2T contained MK-7 as the dominant menaquinone.
30230442	6	48	theme	dominant	912:919	arg1	MK-7					900:903	MK-7	900:903	MK-7	900:903	Strain M1HQ-2T contained MK-7 as the dominant menaquinone.
30230442	4	49	theme	diffusible	593:602	arg1	pigments					604:611	diffusible pigments	593:611	diffusible pigments	593:611	Substrate mycelia and aerial mycelia were not formed, and diffusible pigments were not observed on any media tested.
30230442	3	50	theme	w/v	516:518	arg1	NaCl					521:524	0-15 % (w/v) NaCl	508:524	0-15 % (w/v) NaCl (1-3 %)	508:532	Strain M1HQ-2T grew at 4-37 °C (optimum, 30 °C), pH 5.0-11.0 (pH 8.0) and in the presence of 0-15 % (w/v) NaCl (1-3 %).
30230442	3	50	theme	w/v	516:518	arg1	%					531:531	1-3 %	527:531	1-3 %	527:531	Strain M1HQ-2T grew at 4-37 °C (optimum, 30 °C), pH 5.0-11.0 (pH 8.0) and in the presence of 0-15 % (w/v) NaCl (1-3 %).
30230442	1	51	attach	isolated	74:81	arg1	bark					88:91	bark	88:91	bark of Scutellaria baicalensis Georgi	88:125	nov., a novel endophytic actinobacterium isolated from bark of Scutellaria baicalensis Georgi.
30230442	1	51	attach	isolated	74:81	arg2	actinobacterium					58:72	a novel endophytic actinobacterium	39:72	a novel endophytic actinobacterium isolated from bark of Scutellaria baicalensis Georgi	39:125	nov., a novel endophytic actinobacterium isolated from bark of Scutellaria baicalensis Georgi.
30230442	13	52	theme	phylogenetic	1515:1526	arg1	analysis					1528:1535	phylogenetic analysis	1515:1535	phylogenetic analysis	1515:1535	On the basis of phylogenetic analysis, chemotaxonomic characteristics and phenotypic data, strain M1HQ-2T represents a novel species of the genus Brachybacterium, for which the name Brachybacteriumendophyticum sp.
30230442	5	53	theme	%	836:836	arg1	similarity					817:826	the highest 16S rRNA gene sequence similarity	782:826	the highest 16S rRNA gene sequence similarity of 97.6 % to Brachybacteriumsquillarum M-6-3T	782:872	Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain M1HQ-2T belonged to the genus Brachybacterium and had the highest 16S rRNA gene sequence similarity of 97.6 % to Brachybacteriumsquillarum M-6-3T.
30230442	6	54	theme	Strain	875:880	arg1	M1HQ-2T					882:888	Strain M1HQ-2T	875:888	Strain M1HQ-2T	875:888	Strain M1HQ-2T contained MK-7 as the dominant menaquinone.
30230442	10	55	theme	DNA	1227:1229	arg1	%					1273:1273	71.0 mol%	1265:1273	71.0 mol%	1265:1273	The DNA G+C content of strain M1HQ-2T was 71.0 mol%.
30230442	10	55	theme	DNA	1227:1229	arg1	G+C content					1231:1241	The DNA G+C content	1223:1241	The DNA G+C content of strain M1HQ-2T	1223:1259	The DNA G+C content of strain M1HQ-2T was 71.0 mol%.
30230442	12	56	dep	strain	1442:1447	arg1	M1HQ-2T					1449:1455	M1HQ-2T	1449:1455	M1HQ-2T	1449:1455	The estimated DNA-DNA hybridization value between strain M1HQ-2T and type strain of B. sacelli was 20.6 %.
30230442	2	57	theme	Scutellaria	278:288	arg1	Georgi					302:307	Scutellaria baicalensis Georgi	278:307	Scutellaria baicalensis Georgi collected from Guizhou, China	278:337	A Gram-positive, aerobic, coccus-shaped, non-spore-forming actinobacterium, designated strain M1HQ-2T, was isolated from a surface-sterilized bark of Scutellaria baicalensis Georgi collected from Guizhou, China and tested using a polyphasic approach to determine its taxonomic position.
30230442	5	58	theme	Brachybacteriumsquillarum	841:865	arg1	M-6-3T					867:872	Brachybacteriumsquillarum M-6-3T	841:872	Brachybacteriumsquillarum M-6-3T	841:872	Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain M1HQ-2T belonged to the genus Brachybacterium and had the highest 16S rRNA gene sequence similarity of 97.6 % to Brachybacteriumsquillarum M-6-3T.
30230442	13	59	theme	phenotypic	1573:1582	arg1	data					1584:1587	phenotypic data	1573:1587	phenotypic data	1573:1587	On the basis of phylogenetic analysis, chemotaxonomic characteristics and phenotypic data, strain M1HQ-2T represents a novel species of the genus Brachybacterium, for which the name Brachybacteriumendophyticum sp.
30230442	1	60	dep	actinobacterium	58:72	arg1	nov.					33:36	nov.	33:36	nov.	33:36	nov., a novel endophytic actinobacterium isolated from bark of Scutellaria baicalensis Georgi.
30230442	15	61	theme	type	1735:1738	arg1	strain					1740:1745	The type strain	1731:1745	The type strain	1731:1745	The type strain is M1HQ-2T (=KCTC 49087T=CGMCC 1.16391T).
30230442	15	61	theme	type	1735:1738	arg1	M1HQ-2T					1750:1756	M1HQ-2T	1750:1756	M1HQ-2T (=KCTC 49087T=CGMCC 1.16391T)	1750:1786	The type strain is M1HQ-2T (=KCTC 49087T=CGMCC 1.16391T).
30230442	5	62	theme	16S	683:685	arg1	rRNA					687:690	16S rRNA	683:690	16S rRNA gene sequence	683:704	Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain M1HQ-2T belonged to the genus Brachybacterium and had the highest 16S rRNA gene sequence similarity of 97.6 % to Brachybacteriumsquillarum M-6-3T.
30230442	7	63	theme	cell-wall	938:946	arg1	peptidoglycan					948:960	The cell-wall peptidoglycan	934:960	The cell-wall peptidoglycan	934:960	The cell-wall peptidoglycan contained meso-diaminopimelic acid.
30230442	5	64	theme	sequence	808:815	arg1	similarity					817:826	the highest 16S rRNA gene sequence similarity	782:826	the highest 16S rRNA gene sequence similarity of 97.6 % to Brachybacteriumsquillarum M-6-3T	782:872	Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain M1HQ-2T belonged to the genus Brachybacterium and had the highest 16S rRNA gene sequence similarity of 97.6 % to Brachybacteriumsquillarum M-6-3T.
30230442	10	65	theme	M1HQ-2T	1253:1259	arg1	%					1273:1273	71.0 mol%	1265:1273	71.0 mol%	1265:1273	The DNA G+C content of strain M1HQ-2T was 71.0 mol%.
30230442	10	65	theme	M1HQ-2T	1253:1259	arg1	G+C content					1231:1241	The DNA G+C content	1223:1241	The DNA G+C content of strain M1HQ-2T	1223:1259	The DNA G+C content of strain M1HQ-2T was 71.0 mol%.
30230442	5	66	theme	gene	692:695	arg1	sequence					697:704	16S rRNA gene sequence	683:704	16S rRNA gene sequence	683:704	Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain M1HQ-2T belonged to the genus Brachybacterium and had the highest 16S rRNA gene sequence similarity of 97.6 % to Brachybacteriumsquillarum M-6-3T.
30230442	5	67	theme	Phylogenetic	652:663	arg1	analysis					665:672	Phylogenetic analysis	652:672	Phylogenetic analysis based on 16S rRNA gene sequence	652:704	Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain M1HQ-2T belonged to the genus Brachybacterium and had the highest 16S rRNA gene sequence similarity of 97.6 % to Brachybacteriumsquillarum M-6-3T.
30230442	8	68	theme	lipids	1008:1013	arg1	profile					1015:1021	The polar lipids profile	998:1021	The polar lipids profile of strain M1HQ-2T	998:1039	The polar lipids profile of strain M1HQ-2T contained diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid and an unidentified lipid.
30230442	1	69	theme	endophytic	47:56	arg1	actinobacterium					58:72	a novel endophytic actinobacterium	39:72	a novel endophytic actinobacterium isolated from bark of Scutellaria baicalensis Georgi	39:125	nov., a novel endophytic actinobacterium isolated from bark of Scutellaria baicalensis Georgi.
30230442	4	70	located	observed	622:629	arg2	pigments					604:611	diffusible pigments	593:611	diffusible pigments	593:611	Substrate mycelia and aerial mycelia were not formed, and diffusible pigments were not observed on any media tested.
30230442	4	70	located	observed	622:629	arg1	media					638:642	any media	634:642	any media tested	634:649	Substrate mycelia and aerial mycelia were not formed, and diffusible pigments were not observed on any media tested.
30230442	11	71	theme	identity	1299:1306	arg1	value					1308:1312	The average nucleotide identity value	1276:1312	The average nucleotide identity value between strain M1HQ-2T and type strain of Brachybacterium sacelli	1276:1378	The average nucleotide identity value between strain M1HQ-2T and type strain of Brachybacterium sacelli was 76.7 %.
30230442	11	71	theme	identity	1299:1306	arg1	%					1389:1389	76.7 %	1384:1389	76.7 %	1384:1389	The average nucleotide identity value between strain M1HQ-2T and type strain of Brachybacterium sacelli was 76.7 %.
30230442	10	72	theme	71.0 mol	1265:1272	arg1	%					1273:1273	71.0 mol%	1265:1273	71.0 mol%	1265:1273	The DNA G+C content of strain M1HQ-2T was 71.0 mol%.
30230442	10	72	theme	71.0 mol	1265:1272	arg1	G+C content					1231:1241	The DNA G+C content	1223:1241	The DNA G+C content of strain M1HQ-2T	1223:1259	The DNA G+C content of strain M1HQ-2T was 71.0 mol%.
30230442	0	73	theme	endophyticum	16:27	arg1	sp					29:30	Brachybacterium endophyticum sp	0:30	Brachybacterium endophyticum sp.	0:31	Brachybacterium endophyticum sp.
30230442	8	74	theme	M1HQ-2T	1033:1039	arg1	profile					1015:1021	The polar lipids profile	998:1021	The polar lipids profile of strain M1HQ-2T	998:1039	The polar lipids profile of strain M1HQ-2T contained diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid and an unidentified lipid.
30230442	3	75	theme	Strain	415:420	arg1	M1HQ-2T					422:428	Strain M1HQ-2T	415:428	Strain M1HQ-2T	415:428	Strain M1HQ-2T grew at 4-37 °C (optimum, 30 °C), pH 5.0-11.0 (pH 8.0) and in the presence of 0-15 % (w/v) NaCl (1-3 %).
30230442	13	76	theme	name	1676:1679	arg1	sp					1709:1710	the name Brachybacteriumendophyticum sp	1672:1710	the name Brachybacteriumendophyticum sp	1672:1710	On the basis of phylogenetic analysis, chemotaxonomic characteristics and phenotypic data, strain M1HQ-2T represents a novel species of the genus Brachybacterium, for which the name Brachybacteriumendophyticum sp.
30230442	15	77	theme	49087T=CGMCC	1765:1776	arg1	1.16391T					1778:1785	=KCTC 49087T=CGMCC 1.16391T	1759:1785	=KCTC 49087T=CGMCC 1.16391T	1759:1785	The type strain is M1HQ-2T (=KCTC 49087T=CGMCC 1.16391T).
30230442	15	77	theme	49087T=CGMCC	1765:1776	arg1	M1HQ-2T					1750:1756	M1HQ-2T	1750:1756	M1HQ-2T (=KCTC 49087T=CGMCC 1.16391T)	1750:1786	The type strain is M1HQ-2T (=KCTC 49087T=CGMCC 1.16391T).
30230442	2	78	theme	taxonomic	395:403	arg1	position					405:412	its taxonomic position	391:412	its taxonomic position	391:412	A Gram-positive, aerobic, coccus-shaped, non-spore-forming actinobacterium, designated strain M1HQ-2T, was isolated from a surface-sterilized bark of Scutellaria baicalensis Georgi collected from Guizhou, China and tested using a polyphasic approach to determine its taxonomic position.
30230442	1	79	theme	Scutellaria	96:106	arg1	Georgi					120:125	Scutellaria baicalensis Georgi	96:125	Scutellaria baicalensis Georgi	96:125	nov., a novel endophytic actinobacterium isolated from bark of Scutellaria baicalensis Georgi.
30230442	13	80	theme	genus	1639:1643	arg1	Brachybacterium					1645:1659	the genus Brachybacterium	1635:1659	the genus Brachybacterium	1635:1659	On the basis of phylogenetic analysis, chemotaxonomic characteristics and phenotypic data, strain M1HQ-2T represents a novel species of the genus Brachybacterium, for which the name Brachybacteriumendophyticum sp.
30230442	5	81	theme	strain	721:726	arg1	M1HQ-2T					728:734	strain M1HQ-2T	721:734	strain M1HQ-2T	721:734	Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain M1HQ-2T belonged to the genus Brachybacterium and had the highest 16S rRNA gene sequence similarity of 97.6 % to Brachybacteriumsquillarum M-6-3T.
30230442	2	82	dep	Gram-positive	130:142	arg1	aerobic					145:151	aerobic	145:151	aerobic	145:151	A Gram-positive, aerobic, coccus-shaped, non-spore-forming actinobacterium, designated strain M1HQ-2T, was isolated from a surface-sterilized bark of Scutellaria baicalensis Georgi collected from Guizhou, China and tested using a polyphasic approach to determine its taxonomic position.
30230442	2	82	dep	Gram-positive	130:142	arg1	non-spore-forming					169:185	non-spore-forming	169:185	non-spore-forming	169:185	A Gram-positive, aerobic, coccus-shaped, non-spore-forming actinobacterium, designated strain M1HQ-2T, was isolated from a surface-sterilized bark of Scutellaria baicalensis Georgi collected from Guizhou, China and tested using a polyphasic approach to determine its taxonomic position.
30230442	2	82	dep	Gram-positive	130:142	arg1	coccus-shaped					154:166	coccus-shaped	154:166	coccus-shaped	154:166	A Gram-positive, aerobic, coccus-shaped, non-spore-forming actinobacterium, designated strain M1HQ-2T, was isolated from a surface-sterilized bark of Scutellaria baicalensis Georgi collected from Guizhou, China and tested using a polyphasic approach to determine its taxonomic position.
30230442	13	83	theme	novel	1618:1622	arg1	species					1624:1630	a novel species	1616:1630	a novel species	1616:1630	On the basis of phylogenetic analysis, chemotaxonomic characteristics and phenotypic data, strain M1HQ-2T represents a novel species of the genus Brachybacterium, for which the name Brachybacteriumendophyticum sp.
30230442	2	84	theme	strain	215:220	arg1	M1HQ-2T					222:228	strain M1HQ-2T	215:228	strain M1HQ-2T	215:228	A Gram-positive, aerobic, coccus-shaped, non-spore-forming actinobacterium, designated strain M1HQ-2T, was isolated from a surface-sterilized bark of Scutellaria baicalensis Georgi collected from Guizhou, China and tested using a polyphasic approach to determine its taxonomic position.
30230442	12	85	theme	B.	1476:1477	arg1	sacelli					1479:1485	B. sacelli	1476:1485	B. sacelli	1476:1485	The estimated DNA-DNA hybridization value between strain M1HQ-2T and type strain of B. sacelli was 20.6 %.
30230442	4	86	theme	aerial	557:562	arg1	mycelia					564:570	aerial mycelia	557:570	aerial mycelia	557:570	Substrate mycelia and aerial mycelia were not formed, and diffusible pigments were not observed on any media tested.
30230442	2	87	theme	Gram-positive	130:142	arg1	actinobacterium					187:201	A Gram-positive, aerobic, coccus-shaped, non-spore-forming actinobacterium	128:201	A Gram-positive, aerobic, coccus-shaped, non-spore-forming actinobacterium	128:201	A Gram-positive, aerobic, coccus-shaped, non-spore-forming actinobacterium, designated strain M1HQ-2T, was isolated from a surface-sterilized bark of Scutellaria baicalensis Georgi collected from Guizhou, China and tested using a polyphasic approach to determine its taxonomic position.
30230442	6	88	contain	contained	890:898	arg1	M1HQ-2T					882:888	Strain M1HQ-2T	875:888	Strain M1HQ-2T	875:888	Strain M1HQ-2T contained MK-7 as the dominant menaquinone.
30230442	6	88	contain	contained	890:898	arg2	menaquinone					921:931	the dominant menaquinone	908:931	the dominant menaquinone	908:931	Strain M1HQ-2T contained MK-7 as the dominant menaquinone.
30230442	6	88	contain	contained	890:898	arg2	MK-7					900:903	MK-7	900:903	MK-7	900:903	Strain M1HQ-2T contained MK-7 as the dominant menaquinone.
30230442	9	89	theme	predominant	1157:1167	arg1	anteiso-C15 					1186:1197	anteiso-C15 	1186:1197	anteiso-C15 	1186:1197	The predominant fatty acids were anteiso-C15 : 0 and anteiso-C17 : 0.
30230442	9	89	theme	predominant	1157:1167	arg1	acids					1175:1179	The predominant fatty acids	1153:1179	The predominant fatty acids	1153:1179	The predominant fatty acids were anteiso-C15 : 0 and anteiso-C17 : 0.
31310194	4	0	attach	isolated	238:245	arg1	roots					252:256	roots	252:256	roots of Azadirachta indica	252:278	A novel endophytic actinomycete strain AZ1-13T was isolated from roots of Azadirachta indica, and its taxonomic position was investigated using a polyphasic approach.
31310194	4	0	attach	isolated	238:245	arg2	AZ1-13T					226:232	A novel endophytic actinomycete strain AZ1-13T	187:232	A novel endophytic actinomycete strain AZ1-13T	187:232	A novel endophytic actinomycete strain AZ1-13T was isolated from roots of Azadirachta indica, and its taxonomic position was investigated using a polyphasic approach.
31310194	12	1	theme	32147T	1783:1788	arg1	2404T					1798:1802	=KCTC 39786T=NBRC 112324T=JCM 32147T = TISTR 2404T	1753:1802	=KCTC 39786T=NBRC 112324T=JCM 32147T = TISTR 2404T	1753:1802	The type strain is AZ1-13T (=KCTC 39786T=NBRC 112324T=JCM 32147T = TISTR 2404T).
31310194	12	1	theme	32147T	1783:1788	arg1	AZ1-13T					1744:1750	AZ1-13T	1744:1750	AZ1-13T (=KCTC 39786T=NBRC 112324T=JCM 32147T = TISTR 2404T)	1744:1803	The type strain is AZ1-13T (=KCTC 39786T=NBRC 112324T=JCM 32147T = TISTR 2404T).
31310194	6	2	theme	Phylogenetic	549:560	arg1	analyses					562:569	Phylogenetic analyses	549:569	Phylogenetic analyses of the family Micromonosporaceae based on 16S rRNA gene sequences	549:635	Phylogenetic analyses of the family Micromonosporaceae based on 16S rRNA gene sequences indicated strains AZ1-13T and J. zingiberis PLAI1-1Tare located within the genus Micromonospora.
31310194	8	3	with	%	907:907	arg1	PLAI1-1T					889:896	PLAI1-1T	889:896	PLAI1-1T	889:896	The strain AZ1-13T exhibited ANIb values of 87.4 % with J. zingiberis PLAI1-1T and 85.1 % with M. endophytica 202201T.
31310194	8	3	with	%	907:907	arg1	202201T					929:935	M. endophytica 202201T	914:935	M. endophytica 202201T	914:935	The strain AZ1-13T exhibited ANIb values of 87.4 % with J. zingiberis PLAI1-1T and 85.1 % with M. endophytica 202201T.
31310194	6	4	theme	rRNA	617:620	arg1	sequences					627:635	16S rRNA gene sequences	613:635	16S rRNA gene sequences	613:635	Phylogenetic analyses of the family Micromonosporaceae based on 16S rRNA gene sequences indicated strains AZ1-13T and J. zingiberis PLAI1-1Tare located within the genus Micromonospora.
31310194	9	5	theme	AZ1-13T	979:985	arg1	characteristics					953:967	Chemotaxonomic characteristics	938:967	Chemotaxonomic characteristics of strain AZ1-13T	938:985	Chemotaxonomic characteristics of strain AZ1-13T were consistent within the genus Micromonospora: cell-wall peptidoglycan of the strain contained meso-diaminopimelic acid; glucose, mannose, ribose and xylose are presented as the whole-cell sugars; the predominant menaquinones were MK-9(H4) and MK-9(H6); major cellular fatty acids were iso-C15 : 0, 10-methyl C17 : 0, C17 : 0, anteiso-C17 : 0 and iso-C17 : 1ω8c; diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol were detected as distinguished phospholipids.
31310194	2	6	theme	Micromonospora	150:163	arg1	comb					176:179	Micromonospora zingiberis comb	150:179	Micromonospora zingiberis comb	150:179	siamensis Valenton, and reclassification of Jishengella zingiberis as Micromonospora zingiberis comb.
31310194	2	7	theme	zingiberis	136:145	arg1	reclassification					104:119	reclassification	104:119	reclassification of Jishengella zingiberis as Micromonospora zingiberis comb	104:179	siamensis Valenton, and reclassification of Jishengella zingiberis as Micromonospora zingiberis comb.
31310194	6	8	theme	gene	622:625	arg1	sequences					627:635	16S rRNA gene sequences	613:635	16S rRNA gene sequences	613:635	Phylogenetic analyses of the family Micromonosporaceae based on 16S rRNA gene sequences indicated strains AZ1-13T and J. zingiberis PLAI1-1Tare located within the genus Micromonospora.
31310194	4	9	theme	endophytic	195:204	arg1	AZ1-13T					226:232	A novel endophytic actinomycete strain AZ1-13T	187:232	A novel endophytic actinomycete strain AZ1-13T	187:232	A novel endophytic actinomycete strain AZ1-13T was isolated from roots of Azadirachta indica, and its taxonomic position was investigated using a polyphasic approach.
31310194	12	10	theme	type	1729:1732	arg1	AZ1-13T					1744:1750	AZ1-13T	1744:1750	AZ1-13T (=KCTC 39786T=NBRC 112324T=JCM 32147T = TISTR 2404T)	1744:1803	The type strain is AZ1-13T (=KCTC 39786T=NBRC 112324T=JCM 32147T = TISTR 2404T).
31310194	12	10	theme	type	1729:1732	arg1	strain					1734:1739	The type strain	1725:1739	The type strain	1725:1739	The type strain is AZ1-13T (=KCTC 39786T=NBRC 112324T=JCM 32147T = TISTR 2404T).
31310194	6	11	theme	16S	613:615	arg1	sequences					627:635	16S rRNA gene sequences	613:635	16S rRNA gene sequences	613:635	Phylogenetic analyses of the family Micromonosporaceae based on 16S rRNA gene sequences indicated strains AZ1-13T and J. zingiberis PLAI1-1Tare located within the genus Micromonospora.
31310194	7	12	theme	approximate	738:748	arg1	size					757:760	The approximate genome size	734:760	The approximate genome size of the strain	734:774	The approximate genome size of the strain was 5.96 Mb with 71.9 mol% of G+C content.
31310194	8	13	theme	M.	914:915	arg1	202201T					929:935	M. endophytica 202201T	914:935	M. endophytica 202201T	914:935	The strain AZ1-13T exhibited ANIb values of 87.4 % with J. zingiberis PLAI1-1T and 85.1 % with M. endophytica 202201T.
31310194	5	14	theme	16S	363:365	arg1	rRNA					367:370	16S rRNA	363:370	Pairwise 16S rRNA gene sequence similarities of strain AZ1-13T	354:415	Pairwise 16S rRNA gene sequence similarities of strain AZ1-13T and its closest species, Jishegella zingiberis PLAI1-1T and Micromonospora endophytica 202201T, were 99.7 and 99.2 %, respectively.
31310194	9	15	theme	cell-wall	1036:1044	arg1	peptidoglycan					1046:1058	cell-wall peptidoglycan	1036:1058	cell-wall peptidoglycan of the strain	1036:1072	Chemotaxonomic characteristics of strain AZ1-13T were consistent within the genus Micromonospora: cell-wall peptidoglycan of the strain contained meso-diaminopimelic acid; glucose, mannose, ribose and xylose are presented as the whole-cell sugars; the predominant menaquinones were MK-9(H4) and MK-9(H6); major cellular fatty acids were iso-C15 : 0, 10-methyl C17 : 0, C17 : 0, anteiso-C17 : 0 and iso-C17 : 1ω8c; diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol were detected as distinguished phospholipids.
31310194	7	16	theme	strain	769:774	arg1	size					757:760	The approximate genome size	734:760	The approximate genome size of the strain	734:774	The approximate genome size of the strain was 5.96 Mb with 71.9 mol% of G+C content.
31310194	9	17	theme	major	1243:1247	arg1	acids					1264:1268	major cellular fatty acids	1243:1268	major cellular fatty acids	1243:1268	Chemotaxonomic characteristics of strain AZ1-13T were consistent within the genus Micromonospora: cell-wall peptidoglycan of the strain contained meso-diaminopimelic acid; glucose, mannose, ribose and xylose are presented as the whole-cell sugars; the predominant menaquinones were MK-9(H4) and MK-9(H6); major cellular fatty acids were iso-C15 : 0, 10-methyl C17 : 0, C17 : 0, anteiso-C17 : 0 and iso-C17 : 1ω8c; diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol were detected as distinguished phospholipids.
31310194	6	18	theme	family	578:583	arg1	Micromonosporaceae					585:602	the family Micromonosporaceae	574:602	the family Micromonosporaceae based on 16S rRNA gene sequences	574:635	Phylogenetic analyses of the family Micromonosporaceae based on 16S rRNA gene sequences indicated strains AZ1-13T and J. zingiberis PLAI1-1Tare located within the genus Micromonospora.
31310194	9	19	theme	fatty	1258:1262	arg1	acids					1264:1268	major cellular fatty acids	1243:1268	major cellular fatty acids	1243:1268	Chemotaxonomic characteristics of strain AZ1-13T were consistent within the genus Micromonospora: cell-wall peptidoglycan of the strain contained meso-diaminopimelic acid; glucose, mannose, ribose and xylose are presented as the whole-cell sugars; the predominant menaquinones were MK-9(H4) and MK-9(H6); major cellular fatty acids were iso-C15 : 0, 10-methyl C17 : 0, C17 : 0, anteiso-C17 : 0 and iso-C17 : 1ω8c; diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol were detected as distinguished phospholipids.
31310194	13	20	theme	Micromonospora	1895:1908	arg1	comb					1921:1924	Micromonospora zingiberis comb	1895:1924	Micromonospora zingiberis comb	1895:1924	This study also proposed that J. zingiberisis transferred to the genus Micromonospora as Micromonospora zingiberis comb.
31310194	5	21	theme	strain	402:407	arg1	AZ1-13T					409:415	strain AZ1-13T	402:415	strain AZ1-13T	402:415	Pairwise 16S rRNA gene sequence similarities of strain AZ1-13T and its closest species, Jishegella zingiberis PLAI1-1T and Micromonospora endophytica 202201T, were 99.7 and 99.2 %, respectively.
31310194	9	22	theme	meso-diaminopimelic	1084:1102	arg1	acid					1104:1107	meso-diaminopimelic acid	1084:1107	meso-diaminopimelic acid	1084:1107	Chemotaxonomic characteristics of strain AZ1-13T were consistent within the genus Micromonospora: cell-wall peptidoglycan of the strain contained meso-diaminopimelic acid; glucose, mannose, ribose and xylose are presented as the whole-cell sugars; the predominant menaquinones were MK-9(H4) and MK-9(H6); major cellular fatty acids were iso-C15 : 0, 10-methyl C17 : 0, C17 : 0, anteiso-C17 : 0 and iso-C17 : 1ω8c; diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol were detected as distinguished phospholipids.
31310194	4	23	theme	strain	219:224	arg1	AZ1-13T					226:232	A novel endophytic actinomycete strain AZ1-13T	187:232	A novel endophytic actinomycete strain AZ1-13T	187:232	A novel endophytic actinomycete strain AZ1-13T was isolated from roots of Azadirachta indica, and its taxonomic position was investigated using a polyphasic approach.
31310194	8	24	theme	strain	823:828	arg1	AZ1-13T					830:836	The strain AZ1-13T	819:836	The strain AZ1-13T	819:836	The strain AZ1-13T exhibited ANIb values of 87.4 % with J. zingiberis PLAI1-1T and 85.1 % with M. endophytica 202201T.
31310194	5	25	theme	gene	372:375	arg1	similarities					386:397	Pairwise 16S rRNA gene sequence similarities	354:397	Pairwise 16S rRNA gene sequence similarities of strain AZ1-13T	354:415	Pairwise 16S rRNA gene sequence similarities of strain AZ1-13T and its closest species, Jishegella zingiberis PLAI1-1T and Micromonospora endophytica 202201T, were 99.7 and 99.2 %, respectively.
31310194	4	26	theme	taxonomic	289:297	arg1	position					299:306	its taxonomic position	285:306	its taxonomic position	285:306	A novel endophytic actinomycete strain AZ1-13T was isolated from roots of Azadirachta indica, and its taxonomic position was investigated using a polyphasic approach.
31310194	10	27	theme	Micromonospora	1645:1658	arg1	species					1624:1630	a novel species	1616:1630	a novel species	1616:1630	Based on phenotypic properties, phylogeny and genomic data, the strain AZ1-13T could be distinguished from its closest neighbours, representing a novel species of the genus Micromonospora, for which the name Micromonospora radicis sp.
31310194	1	28	theme	indica	68:73	arg1	var					75:77	Azadirachta indica var	56:77	Azadirachta indica var	56:77	nov., isolated from roots of Azadirachta indica var.
31310194	8	29	theme	%	907:907	arg1	values					853:858	ANIb values	848:858	ANIb values of 87.4 % with J. zingiberis PLAI1-1T and 85.1 % with M. endophytica 202201T	848:935	The strain AZ1-13T exhibited ANIb values of 87.4 % with J. zingiberis PLAI1-1T and 85.1 % with M. endophytica 202201T.
31310194	0	30	theme	Micromonospora	0:13	arg1	sp					23:24	Micromonospora radicis sp	0:24	Micromonospora radicis sp.	0:25	Micromonospora radicis sp.
31310194	4	31	theme	indica	273:278	arg1	roots					252:256	roots	252:256	roots of Azadirachta indica	252:278	A novel endophytic actinomycete strain AZ1-13T was isolated from roots of Azadirachta indica, and its taxonomic position was investigated using a polyphasic approach.
31310194	6	32	theme	genus	712:716	arg1	Micromonospora					718:731	the genus Micromonospora	708:731	the genus Micromonospora	708:731	Phylogenetic analyses of the family Micromonosporaceae based on 16S rRNA gene sequences indicated strains AZ1-13T and J. zingiberis PLAI1-1Tare located within the genus Micromonospora.
31310194	14	33	theme	113144T=JCM	1970:1980	arg1	32592T					1982:1987	type strain PLAI1-1T=TBRC 7644T=NBRC 113144T=JCM 32592T	1933:1987	type strain PLAI1-1T=TBRC 7644T=NBRC 113144T=JCM 32592T	1933:1987	nov. (type strain PLAI1-1T=TBRC 7644T=NBRC 113144T=JCM 32592T).
31310194	10	34	theme	phenotypic	1481:1490	arg1	properties					1492:1501	phenotypic properties	1481:1501	phenotypic properties	1481:1501	Based on phenotypic properties, phylogeny and genomic data, the strain AZ1-13T could be distinguished from its closest neighbours, representing a novel species of the genus Micromonospora, for which the name Micromonospora radicis sp.
31310194	7	35	theme	G+C content	806:816	arg1	G+C content					806:816	G+C content	806:816	G+C content	806:816	The approximate genome size of the strain was 5.96 Mb with 71.9 mol% of G+C content.
31310194	7	35	theme	G+C content	806:816	arg1	%					801:801	71.9 mol%	793:801	71.9 mol% of G+C content	793:816	The approximate genome size of the strain was 5.96 Mb with 71.9 mol% of G+C content.
31310194	14	36	theme	PLAI1-1T=TBRC	1945:1957	arg1	32592T					1982:1987	type strain PLAI1-1T=TBRC 7644T=NBRC 113144T=JCM 32592T	1933:1987	type strain PLAI1-1T=TBRC 7644T=NBRC 113144T=JCM 32592T	1933:1987	nov. (type strain PLAI1-1T=TBRC 7644T=NBRC 113144T=JCM 32592T).
31310194	2	37	theme	zingiberis	165:174	arg1	comb					176:179	Micromonospora zingiberis comb	150:179	Micromonospora zingiberis comb	150:179	siamensis Valenton, and reclassification of Jishengella zingiberis as Micromonospora zingiberis comb.
31310194	9	38	theme	whole-cell	1167:1176	arg1	sugars					1178:1183	the whole-cell sugars	1163:1183	the whole-cell sugars	1163:1183	Chemotaxonomic characteristics of strain AZ1-13T were consistent within the genus Micromonospora: cell-wall peptidoglycan of the strain contained meso-diaminopimelic acid; glucose, mannose, ribose and xylose are presented as the whole-cell sugars; the predominant menaquinones were MK-9(H4) and MK-9(H6); major cellular fatty acids were iso-C15 : 0, 10-methyl C17 : 0, C17 : 0, anteiso-C17 : 0 and iso-C17 : 1ω8c; diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol were detected as distinguished phospholipids.
31310194	9	38	theme	whole-cell	1167:1176	arg1	ribose					1128:1133	ribose	1128:1133	ribose	1128:1133	Chemotaxonomic characteristics of strain AZ1-13T were consistent within the genus Micromonospora: cell-wall peptidoglycan of the strain contained meso-diaminopimelic acid; glucose, mannose, ribose and xylose are presented as the whole-cell sugars; the predominant menaquinones were MK-9(H4) and MK-9(H6); major cellular fatty acids were iso-C15 : 0, 10-methyl C17 : 0, C17 : 0, anteiso-C17 : 0 and iso-C17 : 1ω8c; diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol were detected as distinguished phospholipids.
31310194	9	38	theme	whole-cell	1167:1176	arg1	mannose					1119:1125	mannose	1119:1125	mannose	1119:1125	Chemotaxonomic characteristics of strain AZ1-13T were consistent within the genus Micromonospora: cell-wall peptidoglycan of the strain contained meso-diaminopimelic acid; glucose, mannose, ribose and xylose are presented as the whole-cell sugars; the predominant menaquinones were MK-9(H4) and MK-9(H6); major cellular fatty acids were iso-C15 : 0, 10-methyl C17 : 0, C17 : 0, anteiso-C17 : 0 and iso-C17 : 1ω8c; diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol were detected as distinguished phospholipids.
31310194	9	38	theme	whole-cell	1167:1176	arg1	glucose					1110:1116	glucose	1110:1116	glucose	1110:1116	Chemotaxonomic characteristics of strain AZ1-13T were consistent within the genus Micromonospora: cell-wall peptidoglycan of the strain contained meso-diaminopimelic acid; glucose, mannose, ribose and xylose are presented as the whole-cell sugars; the predominant menaquinones were MK-9(H4) and MK-9(H6); major cellular fatty acids were iso-C15 : 0, 10-methyl C17 : 0, C17 : 0, anteiso-C17 : 0 and iso-C17 : 1ω8c; diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol were detected as distinguished phospholipids.
31310194	9	38	theme	whole-cell	1167:1176	arg1	xylose					1139:1144	xylose	1139:1144	xylose	1139:1144	Chemotaxonomic characteristics of strain AZ1-13T were consistent within the genus Micromonospora: cell-wall peptidoglycan of the strain contained meso-diaminopimelic acid; glucose, mannose, ribose and xylose are presented as the whole-cell sugars; the predominant menaquinones were MK-9(H4) and MK-9(H6); major cellular fatty acids were iso-C15 : 0, 10-methyl C17 : 0, C17 : 0, anteiso-C17 : 0 and iso-C17 : 1ω8c; diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol were detected as distinguished phospholipids.
31310194	14	39	theme	type	1933:1936	arg1	32592T					1982:1987	type strain PLAI1-1T=TBRC 7644T=NBRC 113144T=JCM 32592T	1933:1987	type strain PLAI1-1T=TBRC 7644T=NBRC 113144T=JCM 32592T	1933:1987	nov. (type strain PLAI1-1T=TBRC 7644T=NBRC 113144T=JCM 32592T).
31310194	10	40	theme	Micromonospora	1680:1693	arg1	sp					1703:1704	the name Micromonospora radicis sp	1671:1704	the name Micromonospora radicis sp	1671:1704	Based on phenotypic properties, phylogeny and genomic data, the strain AZ1-13T could be distinguished from its closest neighbours, representing a novel species of the genus Micromonospora, for which the name Micromonospora radicis sp.
31310194	12	41	theme	112324T=JCM	1771:1781	arg1	2404T					1798:1802	=KCTC 39786T=NBRC 112324T=JCM 32147T = TISTR 2404T	1753:1802	=KCTC 39786T=NBRC 112324T=JCM 32147T = TISTR 2404T	1753:1802	The type strain is AZ1-13T (=KCTC 39786T=NBRC 112324T=JCM 32147T = TISTR 2404T).
31310194	12	41	theme	112324T=JCM	1771:1781	arg1	AZ1-13T					1744:1750	AZ1-13T	1744:1750	AZ1-13T (=KCTC 39786T=NBRC 112324T=JCM 32147T = TISTR 2404T)	1744:1803	The type strain is AZ1-13T (=KCTC 39786T=NBRC 112324T=JCM 32147T = TISTR 2404T).
31310194	9	42	theme	predominant	1190:1200	arg1	MK-9					1220:1223	MK-9	1220:1223	MK-9(H4)	1220:1227	Chemotaxonomic characteristics of strain AZ1-13T were consistent within the genus Micromonospora: cell-wall peptidoglycan of the strain contained meso-diaminopimelic acid; glucose, mannose, ribose and xylose are presented as the whole-cell sugars; the predominant menaquinones were MK-9(H4) and MK-9(H6); major cellular fatty acids were iso-C15 : 0, 10-methyl C17 : 0, C17 : 0, anteiso-C17 : 0 and iso-C17 : 1ω8c; diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol were detected as distinguished phospholipids.
31310194	9	42	theme	predominant	1190:1200	arg1	menaquinones					1202:1213	the predominant menaquinones	1186:1213	the predominant menaquinones	1186:1213	Chemotaxonomic characteristics of strain AZ1-13T were consistent within the genus Micromonospora: cell-wall peptidoglycan of the strain contained meso-diaminopimelic acid; glucose, mannose, ribose and xylose are presented as the whole-cell sugars; the predominant menaquinones were MK-9(H4) and MK-9(H6); major cellular fatty acids were iso-C15 : 0, 10-methyl C17 : 0, C17 : 0, anteiso-C17 : 0 and iso-C17 : 1ω8c; diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol were detected as distinguished phospholipids.
31310194	12	43	theme	=	1790:1790	arg1	2404T					1798:1802	=KCTC 39786T=NBRC 112324T=JCM 32147T = TISTR 2404T	1753:1802	=KCTC 39786T=NBRC 112324T=JCM 32147T = TISTR 2404T	1753:1802	The type strain is AZ1-13T (=KCTC 39786T=NBRC 112324T=JCM 32147T = TISTR 2404T).
31310194	12	43	theme	=	1790:1790	arg1	AZ1-13T					1744:1750	AZ1-13T	1744:1750	AZ1-13T (=KCTC 39786T=NBRC 112324T=JCM 32147T = TISTR 2404T)	1744:1803	The type strain is AZ1-13T (=KCTC 39786T=NBRC 112324T=JCM 32147T = TISTR 2404T).
31310194	5	44	theme	closest	425:431	arg1	species					433:439	its closest species	421:439	its closest species	421:439	Pairwise 16S rRNA gene sequence similarities of strain AZ1-13T and its closest species, Jishegella zingiberis PLAI1-1T and Micromonospora endophytica 202201T, were 99.7 and 99.2 %, respectively.
31310194	5	44	theme	closest	425:431	arg1	202201T					504:510	202201T	504:510	202201T	504:510	Pairwise 16S rRNA gene sequence similarities of strain AZ1-13T and its closest species, Jishegella zingiberis PLAI1-1T and Micromonospora endophytica 202201T, were 99.7 and 99.2 %, respectively.
31310194	5	44	theme	closest	425:431	arg1	PLAI1-1T					464:471	PLAI1-1T	464:471	PLAI1-1T	464:471	Pairwise 16S rRNA gene sequence similarities of strain AZ1-13T and its closest species, Jishegella zingiberis PLAI1-1T and Micromonospora endophytica 202201T, were 99.7 and 99.2 %, respectively.
31310194	9	45	theme	strain	972:977	arg1	AZ1-13T					979:985	strain AZ1-13T	972:985	strain AZ1-13T	972:985	Chemotaxonomic characteristics of strain AZ1-13T were consistent within the genus Micromonospora: cell-wall peptidoglycan of the strain contained meso-diaminopimelic acid; glucose, mannose, ribose and xylose are presented as the whole-cell sugars; the predominant menaquinones were MK-9(H4) and MK-9(H6); major cellular fatty acids were iso-C15 : 0, 10-methyl C17 : 0, C17 : 0, anteiso-C17 : 0 and iso-C17 : 1ω8c; diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol were detected as distinguished phospholipids.
31310194	12	46	theme	=KCTC	1753:1757	arg1	2404T					1798:1802	=KCTC 39786T=NBRC 112324T=JCM 32147T = TISTR 2404T	1753:1802	=KCTC 39786T=NBRC 112324T=JCM 32147T = TISTR 2404T	1753:1802	The type strain is AZ1-13T (=KCTC 39786T=NBRC 112324T=JCM 32147T = TISTR 2404T).
31310194	12	46	theme	=KCTC	1753:1757	arg1	AZ1-13T					1744:1750	AZ1-13T	1744:1750	AZ1-13T (=KCTC 39786T=NBRC 112324T=JCM 32147T = TISTR 2404T)	1744:1803	The type strain is AZ1-13T (=KCTC 39786T=NBRC 112324T=JCM 32147T = TISTR 2404T).
31310194	8	47	theme	%	868:868	arg1	values					853:858	ANIb values	848:858	ANIb values of 87.4 % with J. zingiberis PLAI1-1T and 85.1 % with M. endophytica 202201T	848:935	The strain AZ1-13T exhibited ANIb values of 87.4 % with J. zingiberis PLAI1-1T and 85.1 % with M. endophytica 202201T.
31310194	2	48	theme	Jishengella	124:134	arg1	zingiberis					136:145	Jishengella zingiberis	124:145	Jishengella zingiberis	124:145	siamensis Valenton, and reclassification of Jishengella zingiberis as Micromonospora zingiberis comb.
31310194	9	49	theme	genus	1014:1018	arg1	Micromonospora					1020:1033	the genus Micromonospora	1010:1033	the genus Micromonospora	1010:1033	Chemotaxonomic characteristics of strain AZ1-13T were consistent within the genus Micromonospora: cell-wall peptidoglycan of the strain contained meso-diaminopimelic acid; glucose, mannose, ribose and xylose are presented as the whole-cell sugars; the predominant menaquinones were MK-9(H4) and MK-9(H6); major cellular fatty acids were iso-C15 : 0, 10-methyl C17 : 0, C17 : 0, anteiso-C17 : 0 and iso-C17 : 1ω8c; diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol were detected as distinguished phospholipids.
31310194	9	50	theme	distinguished	1443:1455	arg1	 1ω8c					1345:1349	 1ω8c	1345:1349	 1ω8c	1345:1349	Chemotaxonomic characteristics of strain AZ1-13T were consistent within the genus Micromonospora: cell-wall peptidoglycan of the strain contained meso-diaminopimelic acid; glucose, mannose, ribose and xylose are presented as the whole-cell sugars; the predominant menaquinones were MK-9(H4) and MK-9(H6); major cellular fatty acids were iso-C15 : 0, 10-methyl C17 : 0, C17 : 0, anteiso-C17 : 0 and iso-C17 : 1ω8c; diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol were detected as distinguished phospholipids.
31310194	9	50	theme	distinguished	1443:1455	arg1	phosphatidylinositol					1405:1424	phosphatidylinositol	1405:1424	phosphatidylinositol	1405:1424	Chemotaxonomic characteristics of strain AZ1-13T were consistent within the genus Micromonospora: cell-wall peptidoglycan of the strain contained meso-diaminopimelic acid; glucose, mannose, ribose and xylose are presented as the whole-cell sugars; the predominant menaquinones were MK-9(H4) and MK-9(H6); major cellular fatty acids were iso-C15 : 0, 10-methyl C17 : 0, C17 : 0, anteiso-C17 : 0 and iso-C17 : 1ω8c; diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol were detected as distinguished phospholipids.
31310194	9	50	theme	distinguished	1443:1455	arg1	 0					1303:1304	 0	1303:1304	 0	1303:1304	Chemotaxonomic characteristics of strain AZ1-13T were consistent within the genus Micromonospora: cell-wall peptidoglycan of the strain contained meso-diaminopimelic acid; glucose, mannose, ribose and xylose are presented as the whole-cell sugars; the predominant menaquinones were MK-9(H4) and MK-9(H6); major cellular fatty acids were iso-C15 : 0, 10-methyl C17 : 0, C17 : 0, anteiso-C17 : 0 and iso-C17 : 1ω8c; diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol were detected as distinguished phospholipids.
31310194	9	50	theme	distinguished	1443:1455	arg1	 0					1329:1330	 0	1329:1330	 0	1329:1330	Chemotaxonomic characteristics of strain AZ1-13T were consistent within the genus Micromonospora: cell-wall peptidoglycan of the strain contained meso-diaminopimelic acid; glucose, mannose, ribose and xylose are presented as the whole-cell sugars; the predominant menaquinones were MK-9(H4) and MK-9(H6); major cellular fatty acids were iso-C15 : 0, 10-methyl C17 : 0, C17 : 0, anteiso-C17 : 0 and iso-C17 : 1ω8c; diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol were detected as distinguished phospholipids.
31310194	9	50	theme	distinguished	1443:1455	arg1	 0					1284:1285	 0	1284:1285	 0	1284:1285	Chemotaxonomic characteristics of strain AZ1-13T were consistent within the genus Micromonospora: cell-wall peptidoglycan of the strain contained meso-diaminopimelic acid; glucose, mannose, ribose and xylose are presented as the whole-cell sugars; the predominant menaquinones were MK-9(H4) and MK-9(H6); major cellular fatty acids were iso-C15 : 0, 10-methyl C17 : 0, C17 : 0, anteiso-C17 : 0 and iso-C17 : 1ω8c; diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol were detected as distinguished phospholipids.
31310194	9	50	theme	distinguished	1443:1455	arg1	diphosphatidylglycerol					1352:1373	diphosphatidylglycerol	1352:1373	diphosphatidylglycerol	1352:1373	Chemotaxonomic characteristics of strain AZ1-13T were consistent within the genus Micromonospora: cell-wall peptidoglycan of the strain contained meso-diaminopimelic acid; glucose, mannose, ribose and xylose are presented as the whole-cell sugars; the predominant menaquinones were MK-9(H4) and MK-9(H6); major cellular fatty acids were iso-C15 : 0, 10-methyl C17 : 0, C17 : 0, anteiso-C17 : 0 and iso-C17 : 1ω8c; diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol were detected as distinguished phospholipids.
31310194	9	50	theme	distinguished	1443:1455	arg1	C17 					1307:1310	C17 	1307:1310	C17 	1307:1310	Chemotaxonomic characteristics of strain AZ1-13T were consistent within the genus Micromonospora: cell-wall peptidoglycan of the strain contained meso-diaminopimelic acid; glucose, mannose, ribose and xylose are presented as the whole-cell sugars; the predominant menaquinones were MK-9(H4) and MK-9(H6); major cellular fatty acids were iso-C15 : 0, 10-methyl C17 : 0, C17 : 0, anteiso-C17 : 0 and iso-C17 : 1ω8c; diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol were detected as distinguished phospholipids.
31310194	9	50	theme	distinguished	1443:1455	arg1	phosphatidylethanolamine					1376:1399	phosphatidylethanolamine	1376:1399	phosphatidylethanolamine	1376:1399	Chemotaxonomic characteristics of strain AZ1-13T were consistent within the genus Micromonospora: cell-wall peptidoglycan of the strain contained meso-diaminopimelic acid; glucose, mannose, ribose and xylose are presented as the whole-cell sugars; the predominant menaquinones were MK-9(H4) and MK-9(H6); major cellular fatty acids were iso-C15 : 0, 10-methyl C17 : 0, C17 : 0, anteiso-C17 : 0 and iso-C17 : 1ω8c; diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol were detected as distinguished phospholipids.
31310194	9	50	theme	distinguished	1443:1455	arg1	phospholipids					1457:1469	distinguished phospholipids	1443:1469	distinguished phospholipids	1443:1469	Chemotaxonomic characteristics of strain AZ1-13T were consistent within the genus Micromonospora: cell-wall peptidoglycan of the strain contained meso-diaminopimelic acid; glucose, mannose, ribose and xylose are presented as the whole-cell sugars; the predominant menaquinones were MK-9(H4) and MK-9(H6); major cellular fatty acids were iso-C15 : 0, 10-methyl C17 : 0, C17 : 0, anteiso-C17 : 0 and iso-C17 : 1ω8c; diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol were detected as distinguished phospholipids.
31310194	9	50	theme	distinguished	1443:1455	arg1	C17 					1298:1301	10-methyl C17 	1288:1301	10-methyl C17 	1288:1301	Chemotaxonomic characteristics of strain AZ1-13T were consistent within the genus Micromonospora: cell-wall peptidoglycan of the strain contained meso-diaminopimelic acid; glucose, mannose, ribose and xylose are presented as the whole-cell sugars; the predominant menaquinones were MK-9(H4) and MK-9(H6); major cellular fatty acids were iso-C15 : 0, 10-methyl C17 : 0, C17 : 0, anteiso-C17 : 0 and iso-C17 : 1ω8c; diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol were detected as distinguished phospholipids.
31310194	4	51	theme	novel	189:193	arg1	AZ1-13T					226:232	A novel endophytic actinomycete strain AZ1-13T	187:232	A novel endophytic actinomycete strain AZ1-13T	187:232	A novel endophytic actinomycete strain AZ1-13T was isolated from roots of Azadirachta indica, and its taxonomic position was investigated using a polyphasic approach.
31310194	5	52	theme	Pairwise	354:361	arg1	similarities					386:397	Pairwise 16S rRNA gene sequence similarities	354:397	Pairwise 16S rRNA gene sequence similarities of strain AZ1-13T	354:415	Pairwise 16S rRNA gene sequence similarities of strain AZ1-13T and its closest species, Jishegella zingiberis PLAI1-1T and Micromonospora endophytica 202201T, were 99.7 and 99.2 %, respectively.
31310194	9	53	contain	contained	1074:1082	arg1	peptidoglycan					1046:1058	cell-wall peptidoglycan	1036:1058	cell-wall peptidoglycan of the strain	1036:1072	Chemotaxonomic characteristics of strain AZ1-13T were consistent within the genus Micromonospora: cell-wall peptidoglycan of the strain contained meso-diaminopimelic acid; glucose, mannose, ribose and xylose are presented as the whole-cell sugars; the predominant menaquinones were MK-9(H4) and MK-9(H6); major cellular fatty acids were iso-C15 : 0, 10-methyl C17 : 0, C17 : 0, anteiso-C17 : 0 and iso-C17 : 1ω8c; diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol were detected as distinguished phospholipids.
31310194	9	53	contain	contained	1074:1082	arg2	acid					1104:1107	meso-diaminopimelic acid	1084:1107	meso-diaminopimelic acid	1084:1107	Chemotaxonomic characteristics of strain AZ1-13T were consistent within the genus Micromonospora: cell-wall peptidoglycan of the strain contained meso-diaminopimelic acid; glucose, mannose, ribose and xylose are presented as the whole-cell sugars; the predominant menaquinones were MK-9(H4) and MK-9(H6); major cellular fatty acids were iso-C15 : 0, 10-methyl C17 : 0, C17 : 0, anteiso-C17 : 0 and iso-C17 : 1ω8c; diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol were detected as distinguished phospholipids.
31310194	14	54	dep	nov	1927:1929	arg1	32592T					1982:1987	type strain PLAI1-1T=TBRC 7644T=NBRC 113144T=JCM 32592T	1933:1987	type strain PLAI1-1T=TBRC 7644T=NBRC 113144T=JCM 32592T	1933:1987	nov. (type strain PLAI1-1T=TBRC 7644T=NBRC 113144T=JCM 32592T).
31310194	10	55	theme	closest	1583:1589	arg1	neighbours					1591:1600	its closest neighbours	1579:1600	its closest neighbours	1579:1600	Based on phenotypic properties, phylogeny and genomic data, the strain AZ1-13T could be distinguished from its closest neighbours, representing a novel species of the genus Micromonospora, for which the name Micromonospora radicis sp.
31310194	6	56	dep	strains	647:653	arg1	AZ1-13T					655:661	AZ1-13T	655:661	AZ1-13T	655:661	Phylogenetic analyses of the family Micromonosporaceae based on 16S rRNA gene sequences indicated strains AZ1-13T and J. zingiberis PLAI1-1Tare located within the genus Micromonospora.
31310194	6	56	dep	strains	647:653	arg1	PLAI1-1Tare					681:691	PLAI1-1Tare	681:691	PLAI1-1Tare	681:691	Phylogenetic analyses of the family Micromonosporaceae based on 16S rRNA gene sequences indicated strains AZ1-13T and J. zingiberis PLAI1-1Tare located within the genus Micromonospora.
31310194	6	56	dep	strains	647:653	arg1	strains					647:653	strains AZ1-13T and J. zingiberis PLAI1-1Tare	647:691	strains AZ1-13T and J. zingiberis PLAI1-1Tare located within the genus Micromonospora	647:731	Phylogenetic analyses of the family Micromonosporaceae based on 16S rRNA gene sequences indicated strains AZ1-13T and J. zingiberis PLAI1-1Tare located within the genus Micromonospora.
31310194	6	57	theme	Micromonosporaceae	585:602	arg1	analyses					562:569	Phylogenetic analyses	549:569	Phylogenetic analyses of the family Micromonosporaceae based on 16S rRNA gene sequences	549:635	Phylogenetic analyses of the family Micromonosporaceae based on 16S rRNA gene sequences indicated strains AZ1-13T and J. zingiberis PLAI1-1Tare located within the genus Micromonospora.
31310194	9	58	theme	cellular	1249:1256	arg1	acids					1264:1268	major cellular fatty acids	1243:1268	major cellular fatty acids	1243:1268	Chemotaxonomic characteristics of strain AZ1-13T were consistent within the genus Micromonospora: cell-wall peptidoglycan of the strain contained meso-diaminopimelic acid; glucose, mannose, ribose and xylose are presented as the whole-cell sugars; the predominant menaquinones were MK-9(H4) and MK-9(H6); major cellular fatty acids were iso-C15 : 0, 10-methyl C17 : 0, C17 : 0, anteiso-C17 : 0 and iso-C17 : 1ω8c; diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol were detected as distinguished phospholipids.
31310194	6	59	theme	located	693:699	arg1	AZ1-13T					655:661	AZ1-13T	655:661	AZ1-13T	655:661	Phylogenetic analyses of the family Micromonosporaceae based on 16S rRNA gene sequences indicated strains AZ1-13T and J. zingiberis PLAI1-1Tare located within the genus Micromonospora.
31310194	6	59	theme	located	693:699	arg1	PLAI1-1Tare					681:691	PLAI1-1Tare	681:691	PLAI1-1Tare	681:691	Phylogenetic analyses of the family Micromonosporaceae based on 16S rRNA gene sequences indicated strains AZ1-13T and J. zingiberis PLAI1-1Tare located within the genus Micromonospora.
31310194	6	59	theme	located	693:699	arg1	strains					647:653	strains AZ1-13T and J. zingiberis PLAI1-1Tare	647:691	strains AZ1-13T and J. zingiberis PLAI1-1Tare located within the genus Micromonospora	647:731	Phylogenetic analyses of the family Micromonosporaceae based on 16S rRNA gene sequences indicated strains AZ1-13T and J. zingiberis PLAI1-1Tare located within the genus Micromonospora.
31310194	8	60	theme	ANIb	848:851	arg1	values					853:858	ANIb values	848:858	ANIb values of 87.4 % with J. zingiberis PLAI1-1T and 85.1 % with M. endophytica 202201T	848:935	The strain AZ1-13T exhibited ANIb values of 87.4 % with J. zingiberis PLAI1-1T and 85.1 % with M. endophytica 202201T.
31310194	8	61	with	%	868:868	arg1	PLAI1-1T					889:896	PLAI1-1T	889:896	PLAI1-1T	889:896	The strain AZ1-13T exhibited ANIb values of 87.4 % with J. zingiberis PLAI1-1T and 85.1 % with M. endophytica 202201T.
31310194	8	61	with	%	868:868	arg1	202201T					929:935	M. endophytica 202201T	914:935	M. endophytica 202201T	914:935	The strain AZ1-13T exhibited ANIb values of 87.4 % with J. zingiberis PLAI1-1T and 85.1 % with M. endophytica 202201T.
31310194	13	62	theme	zingiberis	1910:1919	arg1	comb					1921:1924	Micromonospora zingiberis comb	1895:1924	Micromonospora zingiberis comb	1895:1924	This study also proposed that J. zingiberisis transferred to the genus Micromonospora as Micromonospora zingiberis comb.
31310194	5	63	theme	AZ1-13T	409:415	arg1	species					433:439	its closest species	421:439	its closest species	421:439	Pairwise 16S rRNA gene sequence similarities of strain AZ1-13T and its closest species, Jishegella zingiberis PLAI1-1T and Micromonospora endophytica 202201T, were 99.7 and 99.2 %, respectively.
31310194	5	63	theme	AZ1-13T	409:415	arg1	202201T					504:510	202201T	504:510	202201T	504:510	Pairwise 16S rRNA gene sequence similarities of strain AZ1-13T and its closest species, Jishegella zingiberis PLAI1-1T and Micromonospora endophytica 202201T, were 99.7 and 99.2 %, respectively.
31310194	5	63	theme	AZ1-13T	409:415	arg1	similarities					386:397	Pairwise 16S rRNA gene sequence similarities	354:397	Pairwise 16S rRNA gene sequence similarities of strain AZ1-13T	354:415	Pairwise 16S rRNA gene sequence similarities of strain AZ1-13T and its closest species, Jishegella zingiberis PLAI1-1T and Micromonospora endophytica 202201T, were 99.7 and 99.2 %, respectively.
31310194	5	63	theme	AZ1-13T	409:415	arg1	PLAI1-1T					464:471	PLAI1-1T	464:471	PLAI1-1T	464:471	Pairwise 16S rRNA gene sequence similarities of strain AZ1-13T and its closest species, Jishegella zingiberis PLAI1-1T and Micromonospora endophytica 202201T, were 99.7 and 99.2 %, respectively.
31310194	4	64	theme	actinomycete	206:217	arg1	AZ1-13T					226:232	A novel endophytic actinomycete strain AZ1-13T	187:232	A novel endophytic actinomycete strain AZ1-13T	187:232	A novel endophytic actinomycete strain AZ1-13T was isolated from roots of Azadirachta indica, and its taxonomic position was investigated using a polyphasic approach.
31310194	5	65	theme	rRNA	367:370	arg1	similarities					386:397	Pairwise 16S rRNA gene sequence similarities	354:397	Pairwise 16S rRNA gene sequence similarities of strain AZ1-13T	354:415	Pairwise 16S rRNA gene sequence similarities of strain AZ1-13T and its closest species, Jishegella zingiberis PLAI1-1T and Micromonospora endophytica 202201T, were 99.7 and 99.2 %, respectively.
31310194	13	66	theme	genus	1871:1875	arg1	Micromonospora					1877:1890	the genus Micromonospora	1867:1890	the genus Micromonospora	1867:1890	This study also proposed that J. zingiberisis transferred to the genus Micromonospora as Micromonospora zingiberis comb.
31310194	9	67	theme	10-methyl	1288:1296	arg1	 1ω8c					1345:1349	 1ω8c	1345:1349	 1ω8c	1345:1349	Chemotaxonomic characteristics of strain AZ1-13T were consistent within the genus Micromonospora: cell-wall peptidoglycan of the strain contained meso-diaminopimelic acid; glucose, mannose, ribose and xylose are presented as the whole-cell sugars; the predominant menaquinones were MK-9(H4) and MK-9(H6); major cellular fatty acids were iso-C15 : 0, 10-methyl C17 : 0, C17 : 0, anteiso-C17 : 0 and iso-C17 : 1ω8c; diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol were detected as distinguished phospholipids.
31310194	9	67	theme	10-methyl	1288:1296	arg1	phosphatidylinositol					1405:1424	phosphatidylinositol	1405:1424	phosphatidylinositol	1405:1424	Chemotaxonomic characteristics of strain AZ1-13T were consistent within the genus Micromonospora: cell-wall peptidoglycan of the strain contained meso-diaminopimelic acid; glucose, mannose, ribose and xylose are presented as the whole-cell sugars; the predominant menaquinones were MK-9(H4) and MK-9(H6); major cellular fatty acids were iso-C15 : 0, 10-methyl C17 : 0, C17 : 0, anteiso-C17 : 0 and iso-C17 : 1ω8c; diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol were detected as distinguished phospholipids.
31310194	9	67	theme	10-methyl	1288:1296	arg1	 0					1303:1304	 0	1303:1304	 0	1303:1304	Chemotaxonomic characteristics of strain AZ1-13T were consistent within the genus Micromonospora: cell-wall peptidoglycan of the strain contained meso-diaminopimelic acid; glucose, mannose, ribose and xylose are presented as the whole-cell sugars; the predominant menaquinones were MK-9(H4) and MK-9(H6); major cellular fatty acids were iso-C15 : 0, 10-methyl C17 : 0, C17 : 0, anteiso-C17 : 0 and iso-C17 : 1ω8c; diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol were detected as distinguished phospholipids.
31310194	9	67	theme	10-methyl	1288:1296	arg1	 0					1329:1330	 0	1329:1330	 0	1329:1330	Chemotaxonomic characteristics of strain AZ1-13T were consistent within the genus Micromonospora: cell-wall peptidoglycan of the strain contained meso-diaminopimelic acid; glucose, mannose, ribose and xylose are presented as the whole-cell sugars; the predominant menaquinones were MK-9(H4) and MK-9(H6); major cellular fatty acids were iso-C15 : 0, 10-methyl C17 : 0, C17 : 0, anteiso-C17 : 0 and iso-C17 : 1ω8c; diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol were detected as distinguished phospholipids.
31310194	9	67	theme	10-methyl	1288:1296	arg1	 0					1284:1285	 0	1284:1285	 0	1284:1285	Chemotaxonomic characteristics of strain AZ1-13T were consistent within the genus Micromonospora: cell-wall peptidoglycan of the strain contained meso-diaminopimelic acid; glucose, mannose, ribose and xylose are presented as the whole-cell sugars; the predominant menaquinones were MK-9(H4) and MK-9(H6); major cellular fatty acids were iso-C15 : 0, 10-methyl C17 : 0, C17 : 0, anteiso-C17 : 0 and iso-C17 : 1ω8c; diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol were detected as distinguished phospholipids.
31310194	9	67	theme	10-methyl	1288:1296	arg1	diphosphatidylglycerol					1352:1373	diphosphatidylglycerol	1352:1373	diphosphatidylglycerol	1352:1373	Chemotaxonomic characteristics of strain AZ1-13T were consistent within the genus Micromonospora: cell-wall peptidoglycan of the strain contained meso-diaminopimelic acid; glucose, mannose, ribose and xylose are presented as the whole-cell sugars; the predominant menaquinones were MK-9(H4) and MK-9(H6); major cellular fatty acids were iso-C15 : 0, 10-methyl C17 : 0, C17 : 0, anteiso-C17 : 0 and iso-C17 : 1ω8c; diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol were detected as distinguished phospholipids.
31310194	9	67	theme	10-methyl	1288:1296	arg1	C17 					1307:1310	C17 	1307:1310	C17 	1307:1310	Chemotaxonomic characteristics of strain AZ1-13T were consistent within the genus Micromonospora: cell-wall peptidoglycan of the strain contained meso-diaminopimelic acid; glucose, mannose, ribose and xylose are presented as the whole-cell sugars; the predominant menaquinones were MK-9(H4) and MK-9(H6); major cellular fatty acids were iso-C15 : 0, 10-methyl C17 : 0, C17 : 0, anteiso-C17 : 0 and iso-C17 : 1ω8c; diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol were detected as distinguished phospholipids.
31310194	9	67	theme	10-methyl	1288:1296	arg1	phosphatidylethanolamine					1376:1399	phosphatidylethanolamine	1376:1399	phosphatidylethanolamine	1376:1399	Chemotaxonomic characteristics of strain AZ1-13T were consistent within the genus Micromonospora: cell-wall peptidoglycan of the strain contained meso-diaminopimelic acid; glucose, mannose, ribose and xylose are presented as the whole-cell sugars; the predominant menaquinones were MK-9(H4) and MK-9(H6); major cellular fatty acids were iso-C15 : 0, 10-methyl C17 : 0, C17 : 0, anteiso-C17 : 0 and iso-C17 : 1ω8c; diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol were detected as distinguished phospholipids.
31310194	9	67	theme	10-methyl	1288:1296	arg1	phospholipids					1457:1469	distinguished phospholipids	1443:1469	distinguished phospholipids	1443:1469	Chemotaxonomic characteristics of strain AZ1-13T were consistent within the genus Micromonospora: cell-wall peptidoglycan of the strain contained meso-diaminopimelic acid; glucose, mannose, ribose and xylose are presented as the whole-cell sugars; the predominant menaquinones were MK-9(H4) and MK-9(H6); major cellular fatty acids were iso-C15 : 0, 10-methyl C17 : 0, C17 : 0, anteiso-C17 : 0 and iso-C17 : 1ω8c; diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol were detected as distinguished phospholipids.
31310194	9	67	theme	10-methyl	1288:1296	arg1	C17 					1298:1301	10-methyl C17 	1288:1301	10-methyl C17 	1288:1301	Chemotaxonomic characteristics of strain AZ1-13T were consistent within the genus Micromonospora: cell-wall peptidoglycan of the strain contained meso-diaminopimelic acid; glucose, mannose, ribose and xylose are presented as the whole-cell sugars; the predominant menaquinones were MK-9(H4) and MK-9(H6); major cellular fatty acids were iso-C15 : 0, 10-methyl C17 : 0, C17 : 0, anteiso-C17 : 0 and iso-C17 : 1ω8c; diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol were detected as distinguished phospholipids.
31310194	9	68	theme	strain	1067:1072	arg1	peptidoglycan					1046:1058	cell-wall peptidoglycan	1036:1058	cell-wall peptidoglycan of the strain	1036:1072	Chemotaxonomic characteristics of strain AZ1-13T were consistent within the genus Micromonospora: cell-wall peptidoglycan of the strain contained meso-diaminopimelic acid; glucose, mannose, ribose and xylose are presented as the whole-cell sugars; the predominant menaquinones were MK-9(H4) and MK-9(H6); major cellular fatty acids were iso-C15 : 0, 10-methyl C17 : 0, C17 : 0, anteiso-C17 : 0 and iso-C17 : 1ω8c; diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol were detected as distinguished phospholipids.
31310194	5	69	theme	sequence	377:384	arg1	similarities					386:397	Pairwise 16S rRNA gene sequence similarities	354:397	Pairwise 16S rRNA gene sequence similarities of strain AZ1-13T	354:415	Pairwise 16S rRNA gene sequence similarities of strain AZ1-13T and its closest species, Jishegella zingiberis PLAI1-1T and Micromonospora endophytica 202201T, were 99.7 and 99.2 %, respectively.
31310194	1	70	theme	Azadirachta	56:66	arg1	var					75:77	Azadirachta indica var	56:77	Azadirachta indica var	56:77	nov., isolated from roots of Azadirachta indica var.
31310194	10	71	theme	genus	1639:1643	arg1	Micromonospora					1645:1658	the genus Micromonospora	1635:1658	the genus Micromonospora	1635:1658	Based on phenotypic properties, phylogeny and genomic data, the strain AZ1-13T could be distinguished from its closest neighbours, representing a novel species of the genus Micromonospora, for which the name Micromonospora radicis sp.
31310194	0	72	theme	radicis	15:21	arg1	sp					23:24	Micromonospora radicis sp	0:24	Micromonospora radicis sp.	0:25	Micromonospora radicis sp.
31310194	1	73	theme	var	75:77	arg1	roots					47:51	roots	47:51	roots of Azadirachta indica var	47:77	nov., isolated from roots of Azadirachta indica var.
31310194	7	74	theme	genome	750:755	arg1	size					757:760	The approximate genome size	734:760	The approximate genome size of the strain	734:774	The approximate genome size of the strain was 5.96 Mb with 71.9 mol% of G+C content.
31310194	4	75	theme	Azadirachta	261:271	arg1	indica					273:278	Azadirachta indica	261:278	Azadirachta indica	261:278	A novel endophytic actinomycete strain AZ1-13T was isolated from roots of Azadirachta indica, and its taxonomic position was investigated using a polyphasic approach.
31310194	14	76	theme	7644T=NBRC	1959:1968	arg1	32592T					1982:1987	type strain PLAI1-1T=TBRC 7644T=NBRC 113144T=JCM 32592T	1933:1987	type strain PLAI1-1T=TBRC 7644T=NBRC 113144T=JCM 32592T	1933:1987	nov. (type strain PLAI1-1T=TBRC 7644T=NBRC 113144T=JCM 32592T).
31310194	8	77	theme	endophytica	917:927	arg1	202201T					929:935	M. endophytica 202201T	914:935	M. endophytica 202201T	914:935	The strain AZ1-13T exhibited ANIb values of 87.4 % with J. zingiberis PLAI1-1T and 85.1 % with M. endophytica 202201T.
31310194	10	78	theme	novel	1618:1622	arg1	species					1624:1630	a novel species	1616:1630	a novel species	1616:1630	Based on phenotypic properties, phylogeny and genomic data, the strain AZ1-13T could be distinguished from its closest neighbours, representing a novel species of the genus Micromonospora, for which the name Micromonospora radicis sp.
31310194	9	79	theme	Chemotaxonomic	938:951	arg1	characteristics					953:967	Chemotaxonomic characteristics	938:967	Chemotaxonomic characteristics of strain AZ1-13T	938:985	Chemotaxonomic characteristics of strain AZ1-13T were consistent within the genus Micromonospora: cell-wall peptidoglycan of the strain contained meso-diaminopimelic acid; glucose, mannose, ribose and xylose are presented as the whole-cell sugars; the predominant menaquinones were MK-9(H4) and MK-9(H6); major cellular fatty acids were iso-C15 : 0, 10-methyl C17 : 0, C17 : 0, anteiso-C17 : 0 and iso-C17 : 1ω8c; diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol were detected as distinguished phospholipids.
31310194	14	80	theme	strain	1938:1943	arg1	32592T					1982:1987	type strain PLAI1-1T=TBRC 7644T=NBRC 113144T=JCM 32592T	1933:1987	type strain PLAI1-1T=TBRC 7644T=NBRC 113144T=JCM 32592T	1933:1987	nov. (type strain PLAI1-1T=TBRC 7644T=NBRC 113144T=JCM 32592T).
31310194	12	81	theme	TISTR	1792:1796	arg1	2404T					1798:1802	=KCTC 39786T=NBRC 112324T=JCM 32147T = TISTR 2404T	1753:1802	=KCTC 39786T=NBRC 112324T=JCM 32147T = TISTR 2404T	1753:1802	The type strain is AZ1-13T (=KCTC 39786T=NBRC 112324T=JCM 32147T = TISTR 2404T).
31310194	12	81	theme	TISTR	1792:1796	arg1	AZ1-13T					1744:1750	AZ1-13T	1744:1750	AZ1-13T (=KCTC 39786T=NBRC 112324T=JCM 32147T = TISTR 2404T)	1744:1803	The type strain is AZ1-13T (=KCTC 39786T=NBRC 112324T=JCM 32147T = TISTR 2404T).
31310194	10	82	theme	genomic	1518:1524	arg1	data					1526:1529	genomic data	1518:1529	genomic data	1518:1529	Based on phenotypic properties, phylogeny and genomic data, the strain AZ1-13T could be distinguished from its closest neighbours, representing a novel species of the genus Micromonospora, for which the name Micromonospora radicis sp.
31310194	10	83	theme	radicis	1695:1701	arg1	sp					1703:1704	the name Micromonospora radicis sp	1671:1704	the name Micromonospora radicis sp	1671:1704	Based on phenotypic properties, phylogeny and genomic data, the strain AZ1-13T could be distinguished from its closest neighbours, representing a novel species of the genus Micromonospora, for which the name Micromonospora radicis sp.
31310194	4	84	theme	polyphasic	333:342	arg1	approach					344:351	a polyphasic approach	331:351	a polyphasic approach	331:351	A novel endophytic actinomycete strain AZ1-13T was isolated from roots of Azadirachta indica, and its taxonomic position was investigated using a polyphasic approach.
31310194	10	85	theme	name	1675:1678	arg1	sp					1703:1704	the name Micromonospora radicis sp	1671:1704	the name Micromonospora radicis sp	1671:1704	Based on phenotypic properties, phylogeny and genomic data, the strain AZ1-13T could be distinguished from its closest neighbours, representing a novel species of the genus Micromonospora, for which the name Micromonospora radicis sp.
31310194	7	86	theme	71.9 mol	793:800	arg1	G+C content					806:816	G+C content	806:816	G+C content	806:816	The approximate genome size of the strain was 5.96 Mb with 71.9 mol% of G+C content.
31310194	7	86	theme	71.9 mol	793:800	arg1	%					801:801	71.9 mol%	793:801	71.9 mol% of G+C content	793:816	The approximate genome size of the strain was 5.96 Mb with 71.9 mol% of G+C content.
31310194	12	87	theme	39786T=NBRC	1759:1769	arg1	2404T					1798:1802	=KCTC 39786T=NBRC 112324T=JCM 32147T = TISTR 2404T	1753:1802	=KCTC 39786T=NBRC 112324T=JCM 32147T = TISTR 2404T	1753:1802	The type strain is AZ1-13T (=KCTC 39786T=NBRC 112324T=JCM 32147T = TISTR 2404T).
31310194	12	87	theme	39786T=NBRC	1759:1769	arg1	AZ1-13T					1744:1750	AZ1-13T	1744:1750	AZ1-13T (=KCTC 39786T=NBRC 112324T=JCM 32147T = TISTR 2404T)	1744:1803	The type strain is AZ1-13T (=KCTC 39786T=NBRC 112324T=JCM 32147T = TISTR 2404T).
31310194	10	88	theme	strain	1536:1541	arg1	AZ1-13T					1543:1549	the strain AZ1-13T	1532:1549	the strain AZ1-13T	1532:1549	Based on phenotypic properties, phylogeny and genomic data, the strain AZ1-13T could be distinguished from its closest neighbours, representing a novel species of the genus Micromonospora, for which the name Micromonospora radicis sp.
30767851	9	0	theme	draft	1090:1094	arg1	genome					1096:1101	The draft genome	1086:1101	The draft genome of SKP7-4T	1086:1112	The draft genome of SKP7-4T was 4.68 Mb with 5208 coding sequences with an average G+C content of 43.2 mol%.
30767851	13	1	theme	=JCM	1627:1630	arg1	2596T					1657:1661	=JCM 33205T=KCTC 43014T=TISTR 2596T	1627:1661	=JCM 33205T=KCTC 43014T=TISTR 2596T	1627:1661	The type strain is SKP7-4T (=JCM 33205T=KCTC 43014T=TISTR 2596T).
30767851	13	1	theme	=JCM	1627:1630	arg1	SKP7-4T					1618:1624	SKP7-4T	1618:1624	SKP7-4T (=JCM 33205T=KCTC 43014T=TISTR 2596T)	1618:1662	The type strain is SKP7-4T (=JCM 33205T=KCTC 43014T=TISTR 2596T).
30767851	11	2	theme	novel	1509:1513	arg1	species					1515:1521	a novel species	1507:1521	a novel species of the genus Bacillus and the name Bacillus salacetis sp	1507:1578	On the basis of the results of phenotypic, chemotaxonomic and phylogenetic analyses, the strain should represent a novel species of the genus Bacillus and the name Bacillus salacetis sp.
30767851	5	3	theme	major	529:533	arg1	Menaquinone					483:493	Menaquinone	483:493	Menaquinone with seven isoprene units	483:519	Menaquinone with seven isoprene units was the major isoprenoid quinone.
30767851	5	3	theme	major	529:533	arg1	quinone					546:552	the major isoprenoid quinone	525:552	the major isoprenoid quinone	525:552	Menaquinone with seven isoprene units was the major isoprenoid quinone.
30767851	8	4	dep	analysis	809:816	arg1	basis					777:781	basis	777:781	basis	777:781	On the basis of 16S rRNA gene sequence analysis, strain SKP7-4T belonged to the genus Bacillus and was closely related to Bacillus vietnamensis JCM 11124T, Bacillus marisflavi JCM 11544T, Bacillus aquimaris JCM 11545T and Bacillusoryzaecorticis JCM 19602T, with 98.7, 97.9, 97.8 and 97.8 % similarity, respectively.
30767851	8	4	dep	analysis	809:816	arg1	the					773:775	the	773:775	the	773:775	On the basis of 16S rRNA gene sequence analysis, strain SKP7-4T belonged to the genus Bacillus and was closely related to Bacillus vietnamensis JCM 11124T, Bacillus marisflavi JCM 11544T, Bacillus aquimaris JCM 11545T and Bacillusoryzaecorticis JCM 19602T, with 98.7, 97.9, 97.8 and 97.8 % similarity, respectively.
30767851	8	5	theme	Bacillus	926:933	arg1	11544T					950:955	Bacillus marisflavi JCM 11544T	926:955	Bacillus marisflavi JCM 11544T	926:955	On the basis of 16S rRNA gene sequence analysis, strain SKP7-4T belonged to the genus Bacillus and was closely related to Bacillus vietnamensis JCM 11124T, Bacillus marisflavi JCM 11544T, Bacillus aquimaris JCM 11545T and Bacillusoryzaecorticis JCM 19602T, with 98.7, 97.9, 97.8 and 97.8 % similarity, respectively.
30767851	7	6	theme	fatty	757:761	arg1	iso-C15 					707:714	iso-C15 	707:714	iso-C15 	707:714	It contained iso-C15 : 0 and anteiso-C15 : 0 as major cellular fatty acids.
30767851	7	6	theme	fatty	757:761	arg1	acids					763:767	major cellular fatty acids	742:767	major cellular fatty acids	742:767	It contained iso-C15 : 0 and anteiso-C15 : 0 as major cellular fatty acids.
30767851	11	7	dep	results	1414:1420	arg1	the					1397:1399	the	1397:1399	the	1397:1399	On the basis of the results of phenotypic, chemotaxonomic and phylogenetic analyses, the strain should represent a novel species of the genus Bacillus and the name Bacillus salacetis sp.
30767851	11	7	dep	results	1414:1420	arg1	basis					1401:1405	basis	1401:1405	basis	1401:1405	On the basis of the results of phenotypic, chemotaxonomic and phylogenetic analyses, the strain should represent a novel species of the genus Bacillus and the name Bacillus salacetis sp.
30767851	9	8	theme	coding	1136:1141	arg1	sequences					1143:1151	5208 coding sequences	1131:1151	5208 coding sequences with an average G+C content of 43.2 mol%	1131:1192	The draft genome of SKP7-4T was 4.68 Mb with 5208 coding sequences with an average G+C content of 43.2 mol%.
30767851	10	9	from	%	1325:1325	arg1	comparison					1330:1339	comparison	1330:1339	comparison with the draft genome of B. vietnamensis JCM 11124T	1330:1391	The ANIb and ANIm values of strain SKP7-4T were 70.0 and 84.3 %, respectively, and the digital DNA-DNA hybridization value was 20 % in comparison with the draft genome of B. vietnamensis JCM 11124T.
30767851	7	10	contain	contained	697:705	arg2	iso-C15 					707:714	iso-C15 	707:714	iso-C15 	707:714	It contained iso-C15 : 0 and anteiso-C15 : 0 as major cellular fatty acids.
30767851	7	10	contain	contained	697:705	arg2	acids					763:767	major cellular fatty acids	742:767	major cellular fatty acids	742:767	It contained iso-C15 : 0 and anteiso-C15 : 0 as major cellular fatty acids.
30767851	7	10	contain	contained	697:705	arg1	It					694:695	It	694:695	It	694:695	It contained iso-C15 : 0 and anteiso-C15 : 0 as major cellular fatty acids.
30767851	9	11	theme	average	1161:1167	arg1	G+C content					1169:1179	an average G+C content	1158:1179	an average G+C content of 43.2 mol%	1158:1192	The draft genome of SKP7-4T was 4.68 Mb with 5208 coding sequences with an average G+C content of 43.2 mol%.
30767851	2	12	theme	shrimp	234:239	arg1	paste					241:245	shrimp paste	234:245	shrimp paste collected from Samut Sakhon province, Thailand	234:292	A Gram-stain-positive, slightly halophilic, endospore-forming, strictly aerobic, rod-shaped bacterium, designated SKP7-4T, was isolated from shrimp paste collected from Samut Sakhon province, Thailand.
30767851	9	13	theme	%	1192:1192	arg1	G+C content					1169:1179	an average G+C content	1158:1179	an average G+C content of 43.2 mol%	1158:1192	The draft genome of SKP7-4T was 4.68 Mb with 5208 coding sequences with an average G+C content of 43.2 mol%.
30767851	8	14	theme	JCM	946:948	arg1	11544T					950:955	Bacillus marisflavi JCM 11544T	926:955	Bacillus marisflavi JCM 11544T	926:955	On the basis of 16S rRNA gene sequence analysis, strain SKP7-4T belonged to the genus Bacillus and was closely related to Bacillus vietnamensis JCM 11124T, Bacillus marisflavi JCM 11544T, Bacillus aquimaris JCM 11545T and Bacillusoryzaecorticis JCM 19602T, with 98.7, 97.9, 97.8 and 97.8 % similarity, respectively.
30767851	8	15	theme	16S	786:788	arg1	analysis					809:816	16S rRNA gene sequence analysis	786:816	16S rRNA gene sequence analysis	786:816	On the basis of 16S rRNA gene sequence analysis, strain SKP7-4T belonged to the genus Bacillus and was closely related to Bacillus vietnamensis JCM 11124T, Bacillus marisflavi JCM 11544T, Bacillus aquimaris JCM 11545T and Bacillusoryzaecorticis JCM 19602T, with 98.7, 97.9, 97.8 and 97.8 % similarity, respectively.
30767851	8	16	dep	similarity	1060:1069	arg1	%					1058:1058	%	1058:1058	%	1058:1058	On the basis of 16S rRNA gene sequence analysis, strain SKP7-4T belonged to the genus Bacillus and was closely related to Bacillus vietnamensis JCM 11124T, Bacillus marisflavi JCM 11544T, Bacillus aquimaris JCM 11545T and Bacillusoryzaecorticis JCM 19602T, with 98.7, 97.9, 97.8 and 97.8 % similarity, respectively.
30767851	10	17	theme	ANIb	1199:1202	arg1	values					1213:1218	The ANIb and ANIm values	1195:1218	values	1213:1218	The ANIb and ANIm values of strain SKP7-4T were 70.0 and 84.3 %, respectively, and the digital DNA-DNA hybridization value was 20 % in comparison with the draft genome of B. vietnamensis JCM 11124T.
30767851	10	17	theme	ANIb	1199:1202	arg1	%					1257:1257	70.0 and 84.3 %	1243:1257	70.0 and 84.3 %	1243:1257	The ANIb and ANIm values of strain SKP7-4T were 70.0 and 84.3 %, respectively, and the digital DNA-DNA hybridization value was 20 % in comparison with the draft genome of B. vietnamensis JCM 11124T.
30767851	8	18	theme	gene	795:798	arg1	analysis					809:816	16S rRNA gene sequence analysis	786:816	16S rRNA gene sequence analysis	786:816	On the basis of 16S rRNA gene sequence analysis, strain SKP7-4T belonged to the genus Bacillus and was closely related to Bacillus vietnamensis JCM 11124T, Bacillus marisflavi JCM 11544T, Bacillus aquimaris JCM 11545T and Bacillusoryzaecorticis JCM 19602T, with 98.7, 97.9, 97.8 and 97.8 % similarity, respectively.
30767851	1	19	theme	Thai	66:69	arg1	paste					78:82	Thai shrimp paste	66:82	Thai shrimp paste (Ka-pi)	66:90	nov., a slightly halophilic bacterium from Thai shrimp paste (Ka-pi).
30767851	1	19	theme	Thai	66:69	arg1	Ka-pi					85:89	Ka-pi	85:89	Ka-pi	85:89	nov., a slightly halophilic bacterium from Thai shrimp paste (Ka-pi).
30767851	6	20	theme	unidentified	636:647	arg1	lipids					686:691	polar lipids	680:691	polar lipids	680:691	Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and three unidentified phospholipids were detected as polar lipids.
30767851	6	20	theme	unidentified	636:647	arg1	phospholipids					649:661	three unidentified phospholipids	630:661	three unidentified phospholipids	630:661	Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and three unidentified phospholipids were detected as polar lipids.
30767851	6	20	theme	unidentified	636:647	arg1	phosphatidylglycerol					579:598	phosphatidylglycerol	579:598	phosphatidylglycerol	579:598	Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and three unidentified phospholipids were detected as polar lipids.
30767851	6	20	theme	unidentified	636:647	arg1	phosphatidylethanolamine					601:624	phosphatidylethanolamine	601:624	phosphatidylethanolamine	601:624	Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and three unidentified phospholipids were detected as polar lipids.
30767851	6	20	theme	unidentified	636:647	arg1	Diphosphatidylglycerol					555:576	Diphosphatidylglycerol	555:576	Diphosphatidylglycerol	555:576	Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and three unidentified phospholipids were detected as polar lipids.
30767851	0	21	theme	Bacillus	0:7	arg1	sp					19:20	Bacillus salacetis sp	0:20	Bacillus salacetis sp.	0:21	Bacillus salacetis sp.
30767851	7	22	theme	major	742:746	arg1	iso-C15 					707:714	iso-C15 	707:714	iso-C15 	707:714	It contained iso-C15 : 0 and anteiso-C15 : 0 as major cellular fatty acids.
30767851	7	22	theme	major	742:746	arg1	acids					763:767	major cellular fatty acids	742:767	major cellular fatty acids	742:767	It contained iso-C15 : 0 and anteiso-C15 : 0 as major cellular fatty acids.
30767851	8	23	theme	strain	819:824	arg1	SKP7-4T					826:832	strain SKP7-4T	819:832	strain SKP7-4T	819:832	On the basis of 16S rRNA gene sequence analysis, strain SKP7-4T belonged to the genus Bacillus and was closely related to Bacillus vietnamensis JCM 11124T, Bacillus marisflavi JCM 11544T, Bacillus aquimaris JCM 11545T and Bacillusoryzaecorticis JCM 19602T, with 98.7, 97.9, 97.8 and 97.8 % similarity, respectively.
30767851	4	24	theme	cell-wall	429:437	arg1	peptidoglycan					439:451	the cell-wall peptidoglycan	425:451	the cell-wall peptidoglycan	425:451	The diamino acid found in the cell-wall peptidoglycan was meso-diaminopimelic acid.
30767851	9	25	with	sequences	1143:1151	arg1	G+C content					1169:1179	an average G+C content	1158:1179	an average G+C content of 43.2 mol%	1158:1192	The draft genome of SKP7-4T was 4.68 Mb with 5208 coding sequences with an average G+C content of 43.2 mol%.
30767851	3	26	theme	w/v	380:382	arg1	NaCl					385:388	0-15 % (w/v) NaCl	372:388	0-15 % (w/v) NaCl (1-3 %)	372:396	Strain SKP7-4T grew at pH 6.0-9.0 (optimum, 7.5), at 20-40 °C (37 °C) and in 0-15 % (w/v) NaCl (1-3 %).
30767851	3	26	theme	w/v	380:382	arg1	%					395:395	1-3 %	391:395	1-3 %	391:395	Strain SKP7-4T grew at pH 6.0-9.0 (optimum, 7.5), at 20-40 °C (37 °C) and in 0-15 % (w/v) NaCl (1-3 %).
30767851	8	27	theme	Bacillusoryzaecorticis	992:1013	arg1	19602T					1019:1024	Bacillusoryzaecorticis JCM 19602T	992:1024	Bacillusoryzaecorticis JCM 19602T	992:1024	On the basis of 16S rRNA gene sequence analysis, strain SKP7-4T belonged to the genus Bacillus and was closely related to Bacillus vietnamensis JCM 11124T, Bacillus marisflavi JCM 11544T, Bacillus aquimaris JCM 11545T and Bacillusoryzaecorticis JCM 19602T, with 98.7, 97.9, 97.8 and 97.8 % similarity, respectively.
30767851	8	28	with	related	881:887	arg1	similarity					1060:1069	98.7, 97.9, 97.8 and 97.8 % similarity	1032:1069	98.7, 97.9, 97.8 and 97.8 % similarity	1032:1069	On the basis of 16S rRNA gene sequence analysis, strain SKP7-4T belonged to the genus Bacillus and was closely related to Bacillus vietnamensis JCM 11124T, Bacillus marisflavi JCM 11544T, Bacillus aquimaris JCM 11545T and Bacillusoryzaecorticis JCM 19602T, with 98.7, 97.9, 97.8 and 97.8 % similarity, respectively.
30767851	3	29	dep	grew	310:313	arg1	optimum					330:336	optimum	330:336	optimum	330:336	Strain SKP7-4T grew at pH 6.0-9.0 (optimum, 7.5), at 20-40 °C (37 °C) and in 0-15 % (w/v) NaCl (1-3 %).
30767851	3	30	theme	%	377:377	arg1	NaCl					385:388	0-15 % (w/v) NaCl	372:388	0-15 % (w/v) NaCl (1-3 %)	372:396	Strain SKP7-4T grew at pH 6.0-9.0 (optimum, 7.5), at 20-40 °C (37 °C) and in 0-15 % (w/v) NaCl (1-3 %).
30767851	3	30	theme	%	377:377	arg1	%					395:395	1-3 %	391:395	1-3 %	391:395	Strain SKP7-4T grew at pH 6.0-9.0 (optimum, 7.5), at 20-40 °C (37 °C) and in 0-15 % (w/v) NaCl (1-3 %).
30767851	7	31	dep	iso-C15 	707:714	arg1	anteiso-C15 					723:734	anteiso-C15 	723:734	anteiso-C15 	723:734	It contained iso-C15 : 0 and anteiso-C15 : 0 as major cellular fatty acids.
30767851	7	31	dep	iso-C15 	707:714	arg1	 0					716:717	 0	716:717	 0	716:717	It contained iso-C15 : 0 and anteiso-C15 : 0 as major cellular fatty acids.
30767851	7	31	dep	iso-C15 	707:714	arg1	 0					736:737	 0	736:737	 0	736:737	It contained iso-C15 : 0 and anteiso-C15 : 0 as major cellular fatty acids.
30767851	10	32	theme	11124T	1386:1391	arg1	genome					1356:1361	the draft genome	1346:1361	the draft genome of B. vietnamensis JCM 11124T	1346:1391	The ANIb and ANIm values of strain SKP7-4T were 70.0 and 84.3 %, respectively, and the digital DNA-DNA hybridization value was 20 % in comparison with the draft genome of B. vietnamensis JCM 11124T.
30767851	11	33	theme	Bacillus	1536:1543	arg1	species					1515:1521	a novel species	1507:1521	a novel species of the genus Bacillus and the name Bacillus salacetis sp	1507:1578	On the basis of the results of phenotypic, chemotaxonomic and phylogenetic analyses, the strain should represent a novel species of the genus Bacillus and the name Bacillus salacetis sp.
30767851	10	34	theme	strain	1223:1228	arg1	SKP7-4T					1230:1236	strain SKP7-4T	1223:1236	strain SKP7-4T	1223:1236	The ANIb and ANIm values of strain SKP7-4T were 70.0 and 84.3 %, respectively, and the digital DNA-DNA hybridization value was 20 % in comparison with the draft genome of B. vietnamensis JCM 11124T.
30767851	10	35	from	%	1257:1257	arg1	comparison					1330:1339	comparison	1330:1339	comparison with the draft genome of B. vietnamensis JCM 11124T	1330:1391	The ANIb and ANIm values of strain SKP7-4T were 70.0 and 84.3 %, respectively, and the digital DNA-DNA hybridization value was 20 % in comparison with the draft genome of B. vietnamensis JCM 11124T.
30767851	10	36	theme	vietnamensis	1369:1380	arg1	11124T					1386:1391	B. vietnamensis JCM 11124T	1366:1391	B. vietnamensis JCM 11124T	1366:1391	The ANIb and ANIm values of strain SKP7-4T were 70.0 and 84.3 %, respectively, and the digital DNA-DNA hybridization value was 20 % in comparison with the draft genome of B. vietnamensis JCM 11124T.
30767851	11	37	theme	sp	1577:1578	arg1	species					1515:1521	a novel species	1507:1521	a novel species of the genus Bacillus and the name Bacillus salacetis sp	1507:1578	On the basis of the results of phenotypic, chemotaxonomic and phylogenetic analyses, the strain should represent a novel species of the genus Bacillus and the name Bacillus salacetis sp.
30767851	10	38	theme	SKP7-4T	1230:1236	arg1	values					1213:1218	The ANIb and ANIm values	1195:1218	values	1213:1218	The ANIb and ANIm values of strain SKP7-4T were 70.0 and 84.3 %, respectively, and the digital DNA-DNA hybridization value was 20 % in comparison with the draft genome of B. vietnamensis JCM 11124T.
30767851	10	38	theme	SKP7-4T	1230:1236	arg1	%					1257:1257	70.0 and 84.3 %	1243:1257	70.0 and 84.3 %	1243:1257	The ANIb and ANIm values of strain SKP7-4T were 70.0 and 84.3 %, respectively, and the digital DNA-DNA hybridization value was 20 % in comparison with the draft genome of B. vietnamensis JCM 11124T.
30767851	13	39	theme	33205T=KCTC	1632:1642	arg1	2596T					1657:1661	=JCM 33205T=KCTC 43014T=TISTR 2596T	1627:1661	=JCM 33205T=KCTC 43014T=TISTR 2596T	1627:1661	The type strain is SKP7-4T (=JCM 33205T=KCTC 43014T=TISTR 2596T).
30767851	13	39	theme	33205T=KCTC	1632:1642	arg1	SKP7-4T					1618:1624	SKP7-4T	1618:1624	SKP7-4T (=JCM 33205T=KCTC 43014T=TISTR 2596T)	1618:1662	The type strain is SKP7-4T (=JCM 33205T=KCTC 43014T=TISTR 2596T).
30767851	11	40	theme	Bacillus	1558:1565	arg1	sp					1577:1578	the name Bacillus salacetis sp	1549:1578	the name Bacillus salacetis sp	1549:1578	On the basis of the results of phenotypic, chemotaxonomic and phylogenetic analyses, the strain should represent a novel species of the genus Bacillus and the name Bacillus salacetis sp.
30767851	1	41	from	paste	78:82	arg1	bacterium					51:59	a slightly halophilic bacterium	29:59	a slightly halophilic bacterium from Thai shrimp paste (Ka-pi)	29:90	nov., a slightly halophilic bacterium from Thai shrimp paste (Ka-pi).
30767851	1	41	from	paste	78:82	arg1	nov.					23:26	nov.	23:26	nov.	23:26	nov., a slightly halophilic bacterium from Thai shrimp paste (Ka-pi).
30767851	11	42	theme	analyses	1469:1476	arg1	results					1414:1420	the results	1410:1420	the results of phenotypic, chemotaxonomic and phylogenetic analyses	1410:1476	On the basis of the results of phenotypic, chemotaxonomic and phylogenetic analyses, the strain should represent a novel species of the genus Bacillus and the name Bacillus salacetis sp.
30767851	8	43	theme	Bacillus	892:899	arg1	11124T					918:923	Bacillus vietnamensis JCM 11124T	892:923	Bacillus vietnamensis JCM 11124T	892:923	On the basis of 16S rRNA gene sequence analysis, strain SKP7-4T belonged to the genus Bacillus and was closely related to Bacillus vietnamensis JCM 11124T, Bacillus marisflavi JCM 11544T, Bacillus aquimaris JCM 11545T and Bacillusoryzaecorticis JCM 19602T, with 98.7, 97.9, 97.8 and 97.8 % similarity, respectively.
30767851	10	44	theme	draft	1350:1354	arg1	genome					1356:1361	the draft genome	1346:1361	the draft genome of B. vietnamensis JCM 11124T	1346:1391	The ANIb and ANIm values of strain SKP7-4T were 70.0 and 84.3 %, respectively, and the digital DNA-DNA hybridization value was 20 % in comparison with the draft genome of B. vietnamensis JCM 11124T.
30767851	8	45	dep	Bacillus	958:965	arg1	aquimaris					967:975	aquimaris	967:975	aquimaris	967:975	On the basis of 16S rRNA gene sequence analysis, strain SKP7-4T belonged to the genus Bacillus and was closely related to Bacillus vietnamensis JCM 11124T, Bacillus marisflavi JCM 11544T, Bacillus aquimaris JCM 11545T and Bacillusoryzaecorticis JCM 19602T, with 98.7, 97.9, 97.8 and 97.8 % similarity, respectively.
30767851	10	46	theme	digital	1282:1288	arg1	hybridization					1298:1310	the digital DNA-DNA hybridization	1278:1310	the digital DNA-DNA hybridization value	1278:1316	The ANIb and ANIm values of strain SKP7-4T were 70.0 and 84.3 %, respectively, and the digital DNA-DNA hybridization value was 20 % in comparison with the draft genome of B. vietnamensis JCM 11124T.
30767851	8	47	theme	JCM	914:916	arg1	11124T					918:923	Bacillus vietnamensis JCM 11124T	892:923	Bacillus vietnamensis JCM 11124T	892:923	On the basis of 16S rRNA gene sequence analysis, strain SKP7-4T belonged to the genus Bacillus and was closely related to Bacillus vietnamensis JCM 11124T, Bacillus marisflavi JCM 11544T, Bacillus aquimaris JCM 11545T and Bacillusoryzaecorticis JCM 19602T, with 98.7, 97.9, 97.8 and 97.8 % similarity, respectively.
30767851	10	48	theme	DNA-DNA	1290:1296	arg1	hybridization					1298:1310	the digital DNA-DNA hybridization	1278:1310	the digital DNA-DNA hybridization value	1278:1316	The ANIb and ANIm values of strain SKP7-4T were 70.0 and 84.3 %, respectively, and the digital DNA-DNA hybridization value was 20 % in comparison with the draft genome of B. vietnamensis JCM 11124T.
30767851	5	49	theme	isoprenoid	535:544	arg1	Menaquinone					483:493	Menaquinone	483:493	Menaquinone with seven isoprene units	483:519	Menaquinone with seven isoprene units was the major isoprenoid quinone.
30767851	5	49	theme	isoprenoid	535:544	arg1	quinone					546:552	the major isoprenoid quinone	525:552	the major isoprenoid quinone	525:552	Menaquinone with seven isoprene units was the major isoprenoid quinone.
30767851	9	50	theme	SKP7-4T	1106:1112	arg1	genome					1096:1101	The draft genome	1086:1101	The draft genome of SKP7-4T	1086:1112	The draft genome of SKP7-4T was 4.68 Mb with 5208 coding sequences with an average G+C content of 43.2 mol%.
30767851	5	51	with	Menaquinone	483:493	arg1	units					515:519	seven isoprene units	500:519	seven isoprene units	500:519	Menaquinone with seven isoprene units was the major isoprenoid quinone.
30767851	8	52	theme	marisflavi	935:944	arg1	11544T					950:955	Bacillus marisflavi JCM 11544T	926:955	Bacillus marisflavi JCM 11544T	926:955	On the basis of 16S rRNA gene sequence analysis, strain SKP7-4T belonged to the genus Bacillus and was closely related to Bacillus vietnamensis JCM 11124T, Bacillus marisflavi JCM 11544T, Bacillus aquimaris JCM 11545T and Bacillusoryzaecorticis JCM 19602T, with 98.7, 97.9, 97.8 and 97.8 % similarity, respectively.
30767851	13	53	theme	type	1603:1606	arg1	SKP7-4T					1618:1624	SKP7-4T	1618:1624	SKP7-4T (=JCM 33205T=KCTC 43014T=TISTR 2596T)	1618:1662	The type strain is SKP7-4T (=JCM 33205T=KCTC 43014T=TISTR 2596T).
30767851	13	53	theme	type	1603:1606	arg1	strain					1608:1613	The type strain	1599:1613	The type strain	1599:1613	The type strain is SKP7-4T (=JCM 33205T=KCTC 43014T=TISTR 2596T).
30767851	2	54	attach	isolated	220:227	arg2	bacterium					185:193	A Gram-stain-positive, slightly halophilic, endospore-forming, strictly aerobic, rod-shaped bacterium	93:193	A Gram-stain-positive, slightly halophilic, endospore-forming, strictly aerobic, rod-shaped bacterium	93:193	A Gram-stain-positive, slightly halophilic, endospore-forming, strictly aerobic, rod-shaped bacterium, designated SKP7-4T, was isolated from shrimp paste collected from Samut Sakhon province, Thailand.
30767851	2	54	attach	isolated	220:227	arg1	paste					241:245	shrimp paste	234:245	shrimp paste collected from Samut Sakhon province, Thailand	234:292	A Gram-stain-positive, slightly halophilic, endospore-forming, strictly aerobic, rod-shaped bacterium, designated SKP7-4T, was isolated from shrimp paste collected from Samut Sakhon province, Thailand.
30767851	9	55	theme	43.2 mol	1184:1191	arg1	%					1192:1192	43.2 mol%	1184:1192	43.2 mol%	1184:1192	The draft genome of SKP7-4T was 4.68 Mb with 5208 coding sequences with an average G+C content of 43.2 mol%.
30767851	3	56	dep	optimum	330:336	arg1	7.5					339:341	7.5	339:341	7.5	339:341	Strain SKP7-4T grew at pH 6.0-9.0 (optimum, 7.5), at 20-40 °C (37 °C) and in 0-15 % (w/v) NaCl (1-3 %).
30767851	4	57	theme	diamino	403:409	arg1	acid					411:414	The diamino acid	399:414	The diamino acid found in the cell-wall peptidoglycan	399:451	The diamino acid found in the cell-wall peptidoglycan was meso-diaminopimelic acid.
30767851	4	57	theme	diamino	403:409	arg1	acid					477:480	meso-diaminopimelic acid	457:480	meso-diaminopimelic acid	457:480	The diamino acid found in the cell-wall peptidoglycan was meso-diaminopimelic acid.
30767851	8	58	dep	%	1058:1058	arg1	97.8 					1053:1057	97.8 	1053:1057	97.8 	1053:1057	On the basis of 16S rRNA gene sequence analysis, strain SKP7-4T belonged to the genus Bacillus and was closely related to Bacillus vietnamensis JCM 11124T, Bacillus marisflavi JCM 11544T, Bacillus aquimaris JCM 11545T and Bacillusoryzaecorticis JCM 19602T, with 98.7, 97.9, 97.8 and 97.8 % similarity, respectively.
30767851	8	58	dep	%	1058:1058	arg1	97.8					1044:1047	97.8	1044:1047	97.8	1044:1047	On the basis of 16S rRNA gene sequence analysis, strain SKP7-4T belonged to the genus Bacillus and was closely related to Bacillus vietnamensis JCM 11124T, Bacillus marisflavi JCM 11544T, Bacillus aquimaris JCM 11545T and Bacillusoryzaecorticis JCM 19602T, with 98.7, 97.9, 97.8 and 97.8 % similarity, respectively.
30767851	8	59	theme	Bacillus	958:965	arg1	11545T					981:986	Bacillus aquimaris JCM 11545T	958:986	Bacillus aquimaris JCM 11545T	958:986	On the basis of 16S rRNA gene sequence analysis, strain SKP7-4T belonged to the genus Bacillus and was closely related to Bacillus vietnamensis JCM 11124T, Bacillus marisflavi JCM 11544T, Bacillus aquimaris JCM 11545T and Bacillusoryzaecorticis JCM 19602T, with 98.7, 97.9, 97.8 and 97.8 % similarity, respectively.
30767851	5	60	theme	isoprene	506:513	arg1	units					515:519	seven isoprene units	500:519	seven isoprene units	500:519	Menaquinone with seven isoprene units was the major isoprenoid quinone.
30767851	1	61	theme	halophilic	40:49	arg1	bacterium					51:59	a slightly halophilic bacterium	29:59	a slightly halophilic bacterium from Thai shrimp paste (Ka-pi)	29:90	nov., a slightly halophilic bacterium from Thai shrimp paste (Ka-pi).
30767851	1	61	theme	halophilic	40:49	arg1	nov.					23:26	nov.	23:26	nov.	23:26	nov., a slightly halophilic bacterium from Thai shrimp paste (Ka-pi).
30767851	10	62	theme	hybridization	1298:1310	arg1	value					1312:1316	the digital DNA-DNA hybridization value	1278:1316	the digital DNA-DNA hybridization value	1278:1316	The ANIb and ANIm values of strain SKP7-4T were 70.0 and 84.3 %, respectively, and the digital DNA-DNA hybridization value was 20 % in comparison with the draft genome of B. vietnamensis JCM 11124T.
30767851	8	63	theme	JCM	977:979	arg1	11545T					981:986	Bacillus aquimaris JCM 11545T	958:986	Bacillus aquimaris JCM 11545T	958:986	On the basis of 16S rRNA gene sequence analysis, strain SKP7-4T belonged to the genus Bacillus and was closely related to Bacillus vietnamensis JCM 11124T, Bacillus marisflavi JCM 11544T, Bacillus aquimaris JCM 11545T and Bacillusoryzaecorticis JCM 19602T, with 98.7, 97.9, 97.8 and 97.8 % similarity, respectively.
30767851	8	64	theme	rRNA	790:793	arg1	analysis					809:816	16S rRNA gene sequence analysis	786:816	16S rRNA gene sequence analysis	786:816	On the basis of 16S rRNA gene sequence analysis, strain SKP7-4T belonged to the genus Bacillus and was closely related to Bacillus vietnamensis JCM 11124T, Bacillus marisflavi JCM 11544T, Bacillus aquimaris JCM 11545T and Bacillusoryzaecorticis JCM 19602T, with 98.7, 97.9, 97.8 and 97.8 % similarity, respectively.
30767851	3	65	theme	0-15 	372:376	arg1	NaCl					385:388	0-15 % (w/v) NaCl	372:388	0-15 % (w/v) NaCl (1-3 %)	372:396	Strain SKP7-4T grew at pH 6.0-9.0 (optimum, 7.5), at 20-40 °C (37 °C) and in 0-15 % (w/v) NaCl (1-3 %).
30767851	3	65	theme	0-15 	372:376	arg1	%					395:395	1-3 %	391:395	1-3 %	391:395	Strain SKP7-4T grew at pH 6.0-9.0 (optimum, 7.5), at 20-40 °C (37 °C) and in 0-15 % (w/v) NaCl (1-3 %).
30767851	0	66	theme	salacetis	9:17	arg1	sp					19:20	Bacillus salacetis sp	0:20	Bacillus salacetis sp.	0:21	Bacillus salacetis sp.
30767851	1	67	theme	shrimp	71:76	arg1	paste					78:82	Thai shrimp paste	66:82	Thai shrimp paste (Ka-pi)	66:90	nov., a slightly halophilic bacterium from Thai shrimp paste (Ka-pi).
30767851	1	67	theme	shrimp	71:76	arg1	Ka-pi					85:89	Ka-pi	85:89	Ka-pi	85:89	nov., a slightly halophilic bacterium from Thai shrimp paste (Ka-pi).
30767851	8	68	theme	sequence	800:807	arg1	analysis					809:816	16S rRNA gene sequence analysis	786:816	16S rRNA gene sequence analysis	786:816	On the basis of 16S rRNA gene sequence analysis, strain SKP7-4T belonged to the genus Bacillus and was closely related to Bacillus vietnamensis JCM 11124T, Bacillus marisflavi JCM 11544T, Bacillus aquimaris JCM 11545T and Bacillusoryzaecorticis JCM 19602T, with 98.7, 97.9, 97.8 and 97.8 % similarity, respectively.
30767851	4	69	located	found	416:420	arg2	acid					477:480	meso-diaminopimelic acid	457:480	meso-diaminopimelic acid	457:480	The diamino acid found in the cell-wall peptidoglycan was meso-diaminopimelic acid.
30767851	4	69	located	found	416:420	arg1	peptidoglycan					439:451	the cell-wall peptidoglycan	425:451	the cell-wall peptidoglycan	425:451	The diamino acid found in the cell-wall peptidoglycan was meso-diaminopimelic acid.
30767851	4	69	located	found	416:420	arg2	acid					411:414	The diamino acid	399:414	The diamino acid found in the cell-wall peptidoglycan	399:451	The diamino acid found in the cell-wall peptidoglycan was meso-diaminopimelic acid.
30767851	10	70	theme	ANIm	1208:1211	arg1	values					1213:1218	The ANIb and ANIm values	1195:1218	values	1213:1218	The ANIb and ANIm values of strain SKP7-4T were 70.0 and 84.3 %, respectively, and the digital DNA-DNA hybridization value was 20 % in comparison with the draft genome of B. vietnamensis JCM 11124T.
30767851	10	70	theme	ANIm	1208:1211	arg1	%					1257:1257	70.0 and 84.3 %	1243:1257	70.0 and 84.3 %	1243:1257	The ANIb and ANIm values of strain SKP7-4T were 70.0 and 84.3 %, respectively, and the digital DNA-DNA hybridization value was 20 % in comparison with the draft genome of B. vietnamensis JCM 11124T.
30767851	7	71	theme	cellular	748:755	arg1	iso-C15 					707:714	iso-C15 	707:714	iso-C15 	707:714	It contained iso-C15 : 0 and anteiso-C15 : 0 as major cellular fatty acids.
30767851	7	71	theme	cellular	748:755	arg1	acids					763:767	major cellular fatty acids	742:767	major cellular fatty acids	742:767	It contained iso-C15 : 0 and anteiso-C15 : 0 as major cellular fatty acids.
30767851	8	72	theme	JCM	1015:1017	arg1	19602T					1019:1024	Bacillusoryzaecorticis JCM 19602T	992:1024	Bacillusoryzaecorticis JCM 19602T	992:1024	On the basis of 16S rRNA gene sequence analysis, strain SKP7-4T belonged to the genus Bacillus and was closely related to Bacillus vietnamensis JCM 11124T, Bacillus marisflavi JCM 11544T, Bacillus aquimaris JCM 11545T and Bacillusoryzaecorticis JCM 19602T, with 98.7, 97.9, 97.8 and 97.8 % similarity, respectively.
30767851	4	73	theme	meso-diaminopimelic	457:475	arg1	acid					477:480	meso-diaminopimelic acid	457:480	meso-diaminopimelic acid	457:480	The diamino acid found in the cell-wall peptidoglycan was meso-diaminopimelic acid.
30767851	4	73	theme	meso-diaminopimelic	457:475	arg1	acid					411:414	The diamino acid	399:414	The diamino acid found in the cell-wall peptidoglycan	399:451	The diamino acid found in the cell-wall peptidoglycan was meso-diaminopimelic acid.
30767851	8	74	dep	Bacillus	892:899	arg1	vietnamensis					901:912	vietnamensis	901:912	vietnamensis	901:912	On the basis of 16S rRNA gene sequence analysis, strain SKP7-4T belonged to the genus Bacillus and was closely related to Bacillus vietnamensis JCM 11124T, Bacillus marisflavi JCM 11544T, Bacillus aquimaris JCM 11545T and Bacillusoryzaecorticis JCM 19602T, with 98.7, 97.9, 97.8 and 97.8 % similarity, respectively.
30767851	10	75	with	comparison	1330:1339	arg1	genome					1356:1361	the draft genome	1346:1361	the draft genome of B. vietnamensis JCM 11124T	1346:1391	The ANIb and ANIm values of strain SKP7-4T were 70.0 and 84.3 %, respectively, and the digital DNA-DNA hybridization value was 20 % in comparison with the draft genome of B. vietnamensis JCM 11124T.
30767851	11	76	theme	chemotaxonomic	1437:1450	arg1	analyses					1469:1476	phenotypic, chemotaxonomic and phylogenetic analyses	1425:1476	phenotypic, chemotaxonomic and phylogenetic analyses	1425:1476	On the basis of the results of phenotypic, chemotaxonomic and phylogenetic analyses, the strain should represent a novel species of the genus Bacillus and the name Bacillus salacetis sp.
30767851	2	77	dep	Gram-stain-positive	95:113	arg1	rod-shaped					174:183	rod-shaped	174:183	rod-shaped	174:183	A Gram-stain-positive, slightly halophilic, endospore-forming, strictly aerobic, rod-shaped bacterium, designated SKP7-4T, was isolated from shrimp paste collected from Samut Sakhon province, Thailand.
30767851	2	77	dep	Gram-stain-positive	95:113	arg1	endospore-forming					137:153	endospore-forming	137:153	endospore-forming	137:153	A Gram-stain-positive, slightly halophilic, endospore-forming, strictly aerobic, rod-shaped bacterium, designated SKP7-4T, was isolated from shrimp paste collected from Samut Sakhon province, Thailand.
30767851	2	77	dep	Gram-stain-positive	95:113	arg1	aerobic					165:171	aerobic	165:171	aerobic	165:171	A Gram-stain-positive, slightly halophilic, endospore-forming, strictly aerobic, rod-shaped bacterium, designated SKP7-4T, was isolated from shrimp paste collected from Samut Sakhon province, Thailand.
30767851	2	77	dep	Gram-stain-positive	95:113	arg1	halophilic					125:134	halophilic	125:134	halophilic	125:134	A Gram-stain-positive, slightly halophilic, endospore-forming, strictly aerobic, rod-shaped bacterium, designated SKP7-4T, was isolated from shrimp paste collected from Samut Sakhon province, Thailand.
30767851	10	78	theme	JCM	1382:1384	arg1	11124T					1386:1391	B. vietnamensis JCM 11124T	1366:1391	B. vietnamensis JCM 11124T	1366:1391	The ANIb and ANIm values of strain SKP7-4T were 70.0 and 84.3 %, respectively, and the digital DNA-DNA hybridization value was 20 % in comparison with the draft genome of B. vietnamensis JCM 11124T.
30767851	11	79	theme	salacetis	1567:1575	arg1	sp					1577:1578	the name Bacillus salacetis sp	1549:1578	the name Bacillus salacetis sp	1549:1578	On the basis of the results of phenotypic, chemotaxonomic and phylogenetic analyses, the strain should represent a novel species of the genus Bacillus and the name Bacillus salacetis sp.
30767851	11	80	theme	phenotypic	1425:1434	arg1	analyses					1469:1476	phenotypic, chemotaxonomic and phylogenetic analyses	1425:1476	phenotypic, chemotaxonomic and phylogenetic analyses	1425:1476	On the basis of the results of phenotypic, chemotaxonomic and phylogenetic analyses, the strain should represent a novel species of the genus Bacillus and the name Bacillus salacetis sp.
30767851	6	81	theme	polar	680:684	arg1	lipids					686:691	polar lipids	680:691	polar lipids	680:691	Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and three unidentified phospholipids were detected as polar lipids.
30767851	6	81	theme	polar	680:684	arg1	Diphosphatidylglycerol					555:576	Diphosphatidylglycerol	555:576	Diphosphatidylglycerol	555:576	Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and three unidentified phospholipids were detected as polar lipids.
30767851	6	81	theme	polar	680:684	arg1	phosphatidylethanolamine					601:624	phosphatidylethanolamine	601:624	phosphatidylethanolamine	601:624	Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and three unidentified phospholipids were detected as polar lipids.
30767851	6	81	theme	polar	680:684	arg1	phospholipids					649:661	three unidentified phospholipids	630:661	three unidentified phospholipids	630:661	Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and three unidentified phospholipids were detected as polar lipids.
30767851	6	81	theme	polar	680:684	arg1	phosphatidylglycerol					579:598	phosphatidylglycerol	579:598	phosphatidylglycerol	579:598	Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and three unidentified phospholipids were detected as polar lipids.
30767851	10	82	theme	B.	1366:1367	arg1	11124T					1386:1391	B. vietnamensis JCM 11124T	1366:1391	B. vietnamensis JCM 11124T	1366:1391	The ANIb and ANIm values of strain SKP7-4T were 70.0 and 84.3 %, respectively, and the digital DNA-DNA hybridization value was 20 % in comparison with the draft genome of B. vietnamensis JCM 11124T.
30767851	11	83	theme	name	1553:1556	arg1	sp					1577:1578	the name Bacillus salacetis sp	1549:1578	the name Bacillus salacetis sp	1549:1578	On the basis of the results of phenotypic, chemotaxonomic and phylogenetic analyses, the strain should represent a novel species of the genus Bacillus and the name Bacillus salacetis sp.
30767851	11	84	dep	Bacillus	1536:1543	arg1	the					1526:1528	the	1526:1528	the	1526:1528	On the basis of the results of phenotypic, chemotaxonomic and phylogenetic analyses, the strain should represent a novel species of the genus Bacillus and the name Bacillus salacetis sp.
30767851	13	85	theme	43014T=TISTR	1644:1655	arg1	2596T					1657:1661	=JCM 33205T=KCTC 43014T=TISTR 2596T	1627:1661	=JCM 33205T=KCTC 43014T=TISTR 2596T	1627:1661	The type strain is SKP7-4T (=JCM 33205T=KCTC 43014T=TISTR 2596T).
30767851	13	85	theme	43014T=TISTR	1644:1655	arg1	SKP7-4T					1618:1624	SKP7-4T	1618:1624	SKP7-4T (=JCM 33205T=KCTC 43014T=TISTR 2596T)	1618:1662	The type strain is SKP7-4T (=JCM 33205T=KCTC 43014T=TISTR 2596T).
30767851	2	86	theme	Gram-stain-positive	95:113	arg1	bacterium					185:193	A Gram-stain-positive, slightly halophilic, endospore-forming, strictly aerobic, rod-shaped bacterium	93:193	A Gram-stain-positive, slightly halophilic, endospore-forming, strictly aerobic, rod-shaped bacterium	93:193	A Gram-stain-positive, slightly halophilic, endospore-forming, strictly aerobic, rod-shaped bacterium, designated SKP7-4T, was isolated from shrimp paste collected from Samut Sakhon province, Thailand.
30767851	11	87	theme	phylogenetic	1456:1467	arg1	analyses					1469:1476	phenotypic, chemotaxonomic and phylogenetic analyses	1425:1476	phenotypic, chemotaxonomic and phylogenetic analyses	1425:1476	On the basis of the results of phenotypic, chemotaxonomic and phylogenetic analyses, the strain should represent a novel species of the genus Bacillus and the name Bacillus salacetis sp.
31511125	8	0	theme	predominant	1086:1096	arg1	acids					1104:1108	The predominant fatty acids	1082:1108	The predominant fatty acids (>10.0 %)	1082:1118	The predominant fatty acids (>10.0 %) were C18 : 1ω9c and iso-C16:0.
31511125	8	0	theme	predominant	1086:1096	arg1	%					1117:1117	>10.0 %	1111:1117	>10.0 %	1111:1117	The predominant fatty acids (>10.0 %) were C18 : 1ω9c and iso-C16:0.
31511125	8	0	theme	predominant	1086:1096	arg1	C18 					1125:1128	C18 	1125:1128	C18 : 1ω9c and iso-C16:0	1125:1148	The predominant fatty acids (>10.0 %) were C18 : 1ω9c and iso-C16:0.
31511125	2	1	theme	Gram-stain-positive	90:108	arg1	HY057					173:177	HY057	173:177	HY057	173:177	Two Gram-stain-positive, irregular rod-shaped (0.2-0.5×1.3-2.5 um) strains, HY056T and HY057, were isolated from the faeces of Equus kiang (the largest of the wild asses) collected at different regions from the Qinghai-Tibetan Plateau of PR China.
31511125	2	1	theme	Gram-stain-positive	90:108	arg1	HY056T					162:167	HY056T	162:167	HY056T	162:167	Two Gram-stain-positive, irregular rod-shaped (0.2-0.5×1.3-2.5 um) strains, HY056T and HY057, were isolated from the faeces of Equus kiang (the largest of the wild asses) collected at different regions from the Qinghai-Tibetan Plateau of PR China.
31511125	2	1	theme	Gram-stain-positive	90:108	arg1	strains					153:159	Two Gram-stain-positive, irregular rod-shaped (0.2-0.5×1.3-2.5 um) strains	86:159	strains	153:159	Two Gram-stain-positive, irregular rod-shaped (0.2-0.5×1.3-2.5 um) strains, HY056T and HY057, were isolated from the faeces of Equus kiang (the largest of the wild asses) collected at different regions from the Qinghai-Tibetan Plateau of PR China.
31511125	4	2	theme	above	788:792	arg1	relatives					807:815	the above four closest relatives	784:815	the above four closest relatives	784:815	The digital DNA-DNA hybridization value of strain HY056T was 96.9 % with strain HY057, but less than 30.0 % with the above four closest relatives.
31511125	2	3	theme	Qinghai-Tibetan	297:311	arg1	Plateau					313:319	the Qinghai-Tibetan Plateau	293:319	the Qinghai-Tibetan Plateau of PR China	293:331	Two Gram-stain-positive, irregular rod-shaped (0.2-0.5×1.3-2.5 um) strains, HY056T and HY057, were isolated from the faeces of Equus kiang (the largest of the wild asses) collected at different regions from the Qinghai-Tibetan Plateau of PR China.
31511125	4	4	with	%	777:777	arg1	relatives					807:815	the above four closest relatives	784:815	the above four closest relatives	784:815	The digital DNA-DNA hybridization value of strain HY056T was 96.9 % with strain HY057, but less than 30.0 % with the above four closest relatives.
31511125	9	5	dep	strains	1175:1181	arg1	strains					1175:1181	strains HY056T and HY057	1175:1198	strains HY056T and HY057	1175:1198	The DNA G+C contents of strains HY056T and HY057 were 68.9 and 69.1 mol%, respectively.
31511125	9	5	dep	strains	1175:1181	arg1	HY057					1194:1198	HY057	1194:1198	HY057	1194:1198	The DNA G+C contents of strains HY056T and HY057 were 68.9 and 69.1 mol%, respectively.
31511125	9	5	dep	strains	1175:1181	arg1	HY056T					1183:1188	HY056T	1183:1188	HY056T	1183:1188	The DNA G+C contents of strains HY056T and HY057 were 68.9 and 69.1 mol%, respectively.
31511125	5	6	theme	predominant	835:845	arg1	MK-8					818:821	MK-8	818:821	MK-8(H4)	818:825	MK-8(H4) was the predominant (91.6 %) respiratory quinone.
31511125	5	6	theme	predominant	835:845	arg1	quinone					868:874	the predominant (91.6 %) respiratory quinone	831:874	the predominant (91.6 %) respiratory quinone	831:874	MK-8(H4) was the predominant (91.6 %) respiratory quinone.
31511125	6	7	contain	contained	891:899	arg1	wall					886:889	The cell wall	877:889	The cell wall	877:889	The cell wall contained ll-2,6-diaminopimelic acid as the diamino acid of the peptidoglycan.
31511125	6	7	contain	contained	891:899	arg2	acid					923:926	ll-2,6-diaminopimelic acid	901:926	ll-2,6-diaminopimelic acid	901:926	The cell wall contained ll-2,6-diaminopimelic acid as the diamino acid of the peptidoglycan.
31511125	6	7	contain	contained	891:899	arg2	acid					943:946	the diamino acid	931:946	the diamino acid of the peptidoglycan	931:967	The cell wall contained ll-2,6-diaminopimelic acid as the diamino acid of the peptidoglycan.
31511125	2	8	theme	kiang	219:223	arg1	faeces					203:208	the faeces	199:208	the faeces of Equus kiang (the largest of the wild asses) collected at different regions from the Qinghai-Tibetan Plateau of PR China	199:331	Two Gram-stain-positive, irregular rod-shaped (0.2-0.5×1.3-2.5 um) strains, HY056T and HY057, were isolated from the faeces of Equus kiang (the largest of the wild asses) collected at different regions from the Qinghai-Tibetan Plateau of PR China.
31511125	4	9	theme	closest	799:805	arg1	relatives					807:815	the above four closest relatives	784:815	the above four closest relatives	784:815	The digital DNA-DNA hybridization value of strain HY056T was 96.9 % with strain HY057, but less than 30.0 % with the above four closest relatives.
31511125	1	10	theme	Equus	47:51	arg1	ass					73:75	Tibetan wild ass	60:75	Tibetan wild ass	60:75	nov., isolated from Equus kiang (Tibetan wild ass) faeces.
31511125	1	10	theme	Equus	47:51	arg1	kiang					53:57	Equus kiang	47:57	Equus kiang (Tibetan wild ass)	47:76	nov., isolated from Equus kiang (Tibetan wild ass) faeces.
31511125	3	11	theme	rRNA	492:495	arg1	sequence					502:509	the 16S rRNA gene sequence	484:509	the 16S rRNA gene sequence	484:509	Phylogenetic analyses indicated that strains HY056T and HY057 belong to the genus Nocardioides by sharing a similarity ranging from 96.3 to 97.0 % in the 16S rRNA gene sequence and forming a distinct cluster with Nocardioides daphniae JCM 16608T, Nocardioides houyundeii 78T, Nocardioides solisilvae Ka25T and Nocardioides gilvus XZ17T.
31511125	2	12	attach	isolated	185:192	arg2	HY057					173:177	HY057	173:177	HY057	173:177	Two Gram-stain-positive, irregular rod-shaped (0.2-0.5×1.3-2.5 um) strains, HY056T and HY057, were isolated from the faeces of Equus kiang (the largest of the wild asses) collected at different regions from the Qinghai-Tibetan Plateau of PR China.
31511125	2	12	attach	isolated	185:192	arg1	faeces					203:208	the faeces	199:208	the faeces of Equus kiang (the largest of the wild asses) collected at different regions from the Qinghai-Tibetan Plateau of PR China	199:331	Two Gram-stain-positive, irregular rod-shaped (0.2-0.5×1.3-2.5 um) strains, HY056T and HY057, were isolated from the faeces of Equus kiang (the largest of the wild asses) collected at different regions from the Qinghai-Tibetan Plateau of PR China.
31511125	2	12	attach	isolated	185:192	arg2	HY056T					162:167	HY056T	162:167	HY056T	162:167	Two Gram-stain-positive, irregular rod-shaped (0.2-0.5×1.3-2.5 um) strains, HY056T and HY057, were isolated from the faeces of Equus kiang (the largest of the wild asses) collected at different regions from the Qinghai-Tibetan Plateau of PR China.
31511125	2	12	attach	isolated	185:192	arg2	strains					153:159	Two Gram-stain-positive, irregular rod-shaped (0.2-0.5×1.3-2.5 um) strains	86:159	strains	153:159	Two Gram-stain-positive, irregular rod-shaped (0.2-0.5×1.3-2.5 um) strains, HY056T and HY057, were isolated from the faeces of Equus kiang (the largest of the wild asses) collected at different regions from the Qinghai-Tibetan Plateau of PR China.
31511125	3	13	theme	gene	497:500	arg1	sequence					502:509	the 16S rRNA gene sequence	484:509	the 16S rRNA gene sequence	484:509	Phylogenetic analyses indicated that strains HY056T and HY057 belong to the genus Nocardioides by sharing a similarity ranging from 96.3 to 97.0 % in the 16S rRNA gene sequence and forming a distinct cluster with Nocardioides daphniae JCM 16608T, Nocardioides houyundeii 78T, Nocardioides solisilvae Ka25T and Nocardioides gilvus XZ17T.
31511125	2	14	theme	0.2-0.5×1.3-2.5 um	133:150	arg1	HY057					173:177	HY057	173:177	HY057	173:177	Two Gram-stain-positive, irregular rod-shaped (0.2-0.5×1.3-2.5 um) strains, HY056T and HY057, were isolated from the faeces of Equus kiang (the largest of the wild asses) collected at different regions from the Qinghai-Tibetan Plateau of PR China.
31511125	2	14	theme	0.2-0.5×1.3-2.5 um	133:150	arg1	HY056T					162:167	HY056T	162:167	HY056T	162:167	Two Gram-stain-positive, irregular rod-shaped (0.2-0.5×1.3-2.5 um) strains, HY056T and HY057, were isolated from the faeces of Equus kiang (the largest of the wild asses) collected at different regions from the Qinghai-Tibetan Plateau of PR China.
31511125	2	14	theme	0.2-0.5×1.3-2.5 um	133:150	arg1	strains					153:159	Two Gram-stain-positive, irregular rod-shaped (0.2-0.5×1.3-2.5 um) strains	86:159	strains	153:159	Two Gram-stain-positive, irregular rod-shaped (0.2-0.5×1.3-2.5 um) strains, HY056T and HY057, were isolated from the faeces of Equus kiang (the largest of the wild asses) collected at different regions from the Qinghai-Tibetan Plateau of PR China.
31511125	5	15	theme	respiratory	856:866	arg1	MK-8					818:821	MK-8	818:821	MK-8(H4)	818:825	MK-8(H4) was the predominant (91.6 %) respiratory quinone.
31511125	5	15	theme	respiratory	856:866	arg1	quinone					868:874	the predominant (91.6 %) respiratory quinone	831:874	the predominant (91.6 %) respiratory quinone	831:874	MK-8(H4) was the predominant (91.6 %) respiratory quinone.
31511125	11	16	theme	4.7563T=JCM	1339:1349	arg1	33399T					1351:1356	=CGMCC 4.7563T=JCM 33399T	1332:1356	=CGMCC 4.7563T=JCM 33399T	1332:1356	Strain HY056T (=CGMCC 4.7563T=JCM 33399T) is assigned as the type strain of a novel species within the genus Nocardioides, for which the name Nocardioidesyefusunii sp.
31511125	11	16	theme	4.7563T=JCM	1339:1349	arg1	HY056T					1324:1329	Strain HY056T	1317:1329	Strain HY056T (=CGMCC 4.7563T=JCM 33399T)	1317:1357	Strain HY056T (=CGMCC 4.7563T=JCM 33399T) is assigned as the type strain of a novel species within the genus Nocardioides, for which the name Nocardioidesyefusunii sp.
31511125	1	17	attach	isolated	33:40	arg1	ass					73:75	Tibetan wild ass	60:75	Tibetan wild ass	60:75	nov., isolated from Equus kiang (Tibetan wild ass) faeces.
31511125	1	17	attach	isolated	33:40	arg2	nov.					27:30	nov.	27:30	nov.	27:30	nov., isolated from Equus kiang (Tibetan wild ass) faeces.
31511125	1	17	attach	isolated	33:40	arg1	kiang					53:57	Equus kiang	47:57	Equus kiang (Tibetan wild ass)	47:76	nov., isolated from Equus kiang (Tibetan wild ass) faeces.
31511125	4	18	theme	DNA-DNA	683:689	arg1	hybridization					691:703	digital DNA-DNA hybridization	675:703	The digital DNA-DNA hybridization value of strain HY056T	671:726	The digital DNA-DNA hybridization value of strain HY056T was 96.9 % with strain HY057, but less than 30.0 % with the above four closest relatives.
31511125	5	19	dep	predominant	835:845	arg1	%					853:853	91.6 %	848:853	91.6 %	848:853	MK-8(H4) was the predominant (91.6 %) respiratory quinone.
31511125	2	20	theme	rod-shaped	121:130	arg1	HY057					173:177	HY057	173:177	HY057	173:177	Two Gram-stain-positive, irregular rod-shaped (0.2-0.5×1.3-2.5 um) strains, HY056T and HY057, were isolated from the faeces of Equus kiang (the largest of the wild asses) collected at different regions from the Qinghai-Tibetan Plateau of PR China.
31511125	2	20	theme	rod-shaped	121:130	arg1	HY056T					162:167	HY056T	162:167	HY056T	162:167	Two Gram-stain-positive, irregular rod-shaped (0.2-0.5×1.3-2.5 um) strains, HY056T and HY057, were isolated from the faeces of Equus kiang (the largest of the wild asses) collected at different regions from the Qinghai-Tibetan Plateau of PR China.
31511125	2	20	theme	rod-shaped	121:130	arg1	strains					153:159	Two Gram-stain-positive, irregular rod-shaped (0.2-0.5×1.3-2.5 um) strains	86:159	strains	153:159	Two Gram-stain-positive, irregular rod-shaped (0.2-0.5×1.3-2.5 um) strains, HY056T and HY057, were isolated from the faeces of Equus kiang (the largest of the wild asses) collected at different regions from the Qinghai-Tibetan Plateau of PR China.
31511125	1	21	theme	Tibetan	60:66	arg1	ass					73:75	Tibetan wild ass	60:75	Tibetan wild ass	60:75	nov., isolated from Equus kiang (Tibetan wild ass) faeces.
31511125	1	21	theme	Tibetan	60:66	arg1	kiang					53:57	Equus kiang	47:57	Equus kiang (Tibetan wild ass)	47:76	nov., isolated from Equus kiang (Tibetan wild ass) faeces.
31511125	0	22	theme	yefusunii	13:21	arg1	sp					23:24	yefusunii sp	13:24	yefusunii sp	13:24	Nocardioides yefusunii sp.
31511125	2	23	theme	irregular	111:119	arg1	HY057					173:177	HY057	173:177	HY057	173:177	Two Gram-stain-positive, irregular rod-shaped (0.2-0.5×1.3-2.5 um) strains, HY056T and HY057, were isolated from the faeces of Equus kiang (the largest of the wild asses) collected at different regions from the Qinghai-Tibetan Plateau of PR China.
31511125	2	23	theme	irregular	111:119	arg1	HY056T					162:167	HY056T	162:167	HY056T	162:167	Two Gram-stain-positive, irregular rod-shaped (0.2-0.5×1.3-2.5 um) strains, HY056T and HY057, were isolated from the faeces of Equus kiang (the largest of the wild asses) collected at different regions from the Qinghai-Tibetan Plateau of PR China.
31511125	2	23	theme	irregular	111:119	arg1	strains					153:159	Two Gram-stain-positive, irregular rod-shaped (0.2-0.5×1.3-2.5 um) strains	86:159	strains	153:159	Two Gram-stain-positive, irregular rod-shaped (0.2-0.5×1.3-2.5 um) strains, HY056T and HY057, were isolated from the faeces of Equus kiang (the largest of the wild asses) collected at different regions from the Qinghai-Tibetan Plateau of PR China.
31511125	11	24	theme	name	1454:1457	arg1	sp					1481:1482	the name Nocardioidesyefusunii sp	1450:1482	the name Nocardioidesyefusunii sp	1450:1482	Strain HY056T (=CGMCC 4.7563T=JCM 33399T) is assigned as the type strain of a novel species within the genus Nocardioides, for which the name Nocardioidesyefusunii sp.
31511125	1	25	theme	wild	68:71	arg1	ass					73:75	Tibetan wild ass	60:75	Tibetan wild ass	60:75	nov., isolated from Equus kiang (Tibetan wild ass) faeces.
31511125	1	25	theme	wild	68:71	arg1	kiang					53:57	Equus kiang	47:57	Equus kiang (Tibetan wild ass)	47:76	nov., isolated from Equus kiang (Tibetan wild ass) faeces.
31511125	11	26	theme	=CGMCC	1332:1337	arg1	33399T					1351:1356	=CGMCC 4.7563T=JCM 33399T	1332:1356	=CGMCC 4.7563T=JCM 33399T	1332:1356	Strain HY056T (=CGMCC 4.7563T=JCM 33399T) is assigned as the type strain of a novel species within the genus Nocardioides, for which the name Nocardioidesyefusunii sp.
31511125	11	26	theme	=CGMCC	1332:1337	arg1	HY056T					1324:1329	Strain HY056T	1317:1329	Strain HY056T (=CGMCC 4.7563T=JCM 33399T)	1317:1357	Strain HY056T (=CGMCC 4.7563T=JCM 33399T) is assigned as the type strain of a novel species within the genus Nocardioides, for which the name Nocardioidesyefusunii sp.
31511125	2	27	theme	China	327:331	arg1	Plateau					313:319	the Qinghai-Tibetan Plateau	293:319	the Qinghai-Tibetan Plateau of PR China	293:331	Two Gram-stain-positive, irregular rod-shaped (0.2-0.5×1.3-2.5 um) strains, HY056T and HY057, were isolated from the faeces of Equus kiang (the largest of the wild asses) collected at different regions from the Qinghai-Tibetan Plateau of PR China.
31511125	3	28	theme	Phylogenetic	334:345	arg1	analyses					347:354	Phylogenetic analyses	334:354	Phylogenetic analyses	334:354	Phylogenetic analyses indicated that strains HY056T and HY057 belong to the genus Nocardioides by sharing a similarity ranging from 96.3 to 97.0 % in the 16S rRNA gene sequence and forming a distinct cluster with Nocardioides daphniae JCM 16608T, Nocardioides houyundeii 78T, Nocardioides solisilvae Ka25T and Nocardioides gilvus XZ17T.
31511125	3	29	dep	strains	371:377	arg1	HY057					390:394	HY057	390:394	HY057	390:394	Phylogenetic analyses indicated that strains HY056T and HY057 belong to the genus Nocardioides by sharing a similarity ranging from 96.3 to 97.0 % in the 16S rRNA gene sequence and forming a distinct cluster with Nocardioides daphniae JCM 16608T, Nocardioides houyundeii 78T, Nocardioides solisilvae Ka25T and Nocardioides gilvus XZ17T.
31511125	3	29	dep	strains	371:377	arg1	HY056T					379:384	HY056T	379:384	HY056T	379:384	Phylogenetic analyses indicated that strains HY056T and HY057 belong to the genus Nocardioides by sharing a similarity ranging from 96.3 to 97.0 % in the 16S rRNA gene sequence and forming a distinct cluster with Nocardioides daphniae JCM 16608T, Nocardioides houyundeii 78T, Nocardioides solisilvae Ka25T and Nocardioides gilvus XZ17T.
31511125	3	29	dep	strains	371:377	arg1	strains					371:377	strains	371:377	strains HY056T and HY057	371:394	Phylogenetic analyses indicated that strains HY056T and HY057 belong to the genus Nocardioides by sharing a similarity ranging from 96.3 to 97.0 % in the 16S rRNA gene sequence and forming a distinct cluster with Nocardioides daphniae JCM 16608T, Nocardioides houyundeii 78T, Nocardioides solisilvae Ka25T and Nocardioides gilvus XZ17T.
31511125	4	30	theme	digital	675:681	arg1	hybridization					691:703	digital DNA-DNA hybridization	675:703	The digital DNA-DNA hybridization value of strain HY056T	671:726	The digital DNA-DNA hybridization value of strain HY056T was 96.9 % with strain HY057, but less than 30.0 % with the above four closest relatives.
31511125	4	31	theme	strain	714:719	arg1	HY056T					721:726	strain HY056T	714:726	strain HY056T	714:726	The digital DNA-DNA hybridization value of strain HY056T was 96.9 % with strain HY057, but less than 30.0 % with the above four closest relatives.
31511125	2	32	dep	kiang	219:223	arg1	largest					230:236	largest	230:236	largest	230:236	Two Gram-stain-positive, irregular rod-shaped (0.2-0.5×1.3-2.5 um) strains, HY056T and HY057, were isolated from the faeces of Equus kiang (the largest of the wild asses) collected at different regions from the Qinghai-Tibetan Plateau of PR China.
31511125	11	33	theme	genus	1420:1424	arg1	Nocardioides					1426:1437	the genus Nocardioides	1416:1437	the genus Nocardioides	1416:1437	Strain HY056T (=CGMCC 4.7563T=JCM 33399T) is assigned as the type strain of a novel species within the genus Nocardioides, for which the name Nocardioidesyefusunii sp.
31511125	3	34	theme	16S	488:490	arg1	rRNA					492:495	the 16S rRNA	484:495	the 16S rRNA gene sequence	484:509	Phylogenetic analyses indicated that strains HY056T and HY057 belong to the genus Nocardioides by sharing a similarity ranging from 96.3 to 97.0 % in the 16S rRNA gene sequence and forming a distinct cluster with Nocardioides daphniae JCM 16608T, Nocardioides houyundeii 78T, Nocardioides solisilvae Ka25T and Nocardioides gilvus XZ17T.
31511125	3	35	theme	genus	410:414	arg1	Nocardioides					416:427	the genus Nocardioides	406:427	the genus Nocardioides by sharing a similarity ranging from 96.3 to 97.0 % in the 16S rRNA gene sequence and forming a distinct cluster with Nocardioides daphniae JCM 16608T, Nocardioides houyundeii 78T, Nocardioides solisilvae Ka25T and Nocardioides gilvus XZ17T	406:668	Phylogenetic analyses indicated that strains HY056T and HY057 belong to the genus Nocardioides by sharing a similarity ranging from 96.3 to 97.0 % in the 16S rRNA gene sequence and forming a distinct cluster with Nocardioides daphniae JCM 16608T, Nocardioides houyundeii 78T, Nocardioides solisilvae Ka25T and Nocardioides gilvus XZ17T.
31511125	7	36	theme	polar	974:978	arg1	lipids					980:985	The polar lipids	970:985	The polar lipids	970:985	The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phospholipid.
31511125	2	37	theme	wild	245:248	arg1	asses					250:254	the wild asses	241:254	the wild asses	241:254	Two Gram-stain-positive, irregular rod-shaped (0.2-0.5×1.3-2.5 um) strains, HY056T and HY057, were isolated from the faeces of Equus kiang (the largest of the wild asses) collected at different regions from the Qinghai-Tibetan Plateau of PR China.
31511125	2	38	theme	PR	324:325	arg1	China					327:331	PR China	324:331	PR China	324:331	Two Gram-stain-positive, irregular rod-shaped (0.2-0.5×1.3-2.5 um) strains, HY056T and HY057, were isolated from the faeces of Equus kiang (the largest of the wild asses) collected at different regions from the Qinghai-Tibetan Plateau of PR China.
31511125	3	39	dep	Nocardioides	547:558	arg1	16608T					573:578	JCM 16608T	569:578	Nocardioides daphniae JCM 16608T	547:578	Phylogenetic analyses indicated that strains HY056T and HY057 belong to the genus Nocardioides by sharing a similarity ranging from 96.3 to 97.0 % in the 16S rRNA gene sequence and forming a distinct cluster with Nocardioides daphniae JCM 16608T, Nocardioides houyundeii 78T, Nocardioides solisilvae Ka25T and Nocardioides gilvus XZ17T.
31511125	3	39	dep	Nocardioides	547:558	arg1	daphniae					560:567	Nocardioides daphniae JCM 16608T	547:578	Nocardioides daphniae JCM 16608T	547:578	Phylogenetic analyses indicated that strains HY056T and HY057 belong to the genus Nocardioides by sharing a similarity ranging from 96.3 to 97.0 % in the 16S rRNA gene sequence and forming a distinct cluster with Nocardioides daphniae JCM 16608T, Nocardioides houyundeii 78T, Nocardioides solisilvae Ka25T and Nocardioides gilvus XZ17T.
31511125	3	40	theme	JCM	569:571	arg1	16608T					573:578	JCM 16608T	569:578	Nocardioides daphniae JCM 16608T	547:578	Phylogenetic analyses indicated that strains HY056T and HY057 belong to the genus Nocardioides by sharing a similarity ranging from 96.3 to 97.0 % in the 16S rRNA gene sequence and forming a distinct cluster with Nocardioides daphniae JCM 16608T, Nocardioides houyundeii 78T, Nocardioides solisilvae Ka25T and Nocardioides gilvus XZ17T.
31511125	3	41	theme	distinct	525:532	arg1	cluster					534:540	a distinct cluster	523:540	a distinct cluster	523:540	Phylogenetic analyses indicated that strains HY056T and HY057 belong to the genus Nocardioides by sharing a similarity ranging from 96.3 to 97.0 % in the 16S rRNA gene sequence and forming a distinct cluster with Nocardioides daphniae JCM 16608T, Nocardioides houyundeii 78T, Nocardioides solisilvae Ka25T and Nocardioides gilvus XZ17T.
31511125	6	42	theme	diamino	935:941	arg1	acid					923:926	ll-2,6-diaminopimelic acid	901:926	ll-2,6-diaminopimelic acid	901:926	The cell wall contained ll-2,6-diaminopimelic acid as the diamino acid of the peptidoglycan.
31511125	6	42	theme	diamino	935:941	arg1	acid					943:946	the diamino acid	931:946	the diamino acid of the peptidoglycan	931:967	The cell wall contained ll-2,6-diaminopimelic acid as the diamino acid of the peptidoglycan.
31511125	11	43	theme	Strain	1317:1322	arg1	strain					1383:1388	the type strain	1374:1388	the type strain of a novel species within the genus Nocardioides, for which the name Nocardioidesyefusunii sp	1374:1482	Strain HY056T (=CGMCC 4.7563T=JCM 33399T) is assigned as the type strain of a novel species within the genus Nocardioides, for which the name Nocardioidesyefusunii sp.
31511125	11	43	theme	Strain	1317:1322	arg1	33399T					1351:1356	=CGMCC 4.7563T=JCM 33399T	1332:1356	=CGMCC 4.7563T=JCM 33399T	1332:1356	Strain HY056T (=CGMCC 4.7563T=JCM 33399T) is assigned as the type strain of a novel species within the genus Nocardioides, for which the name Nocardioidesyefusunii sp.
31511125	11	43	theme	Strain	1317:1322	arg1	HY056T					1324:1329	Strain HY056T	1317:1329	Strain HY056T (=CGMCC 4.7563T=JCM 33399T)	1317:1357	Strain HY056T (=CGMCC 4.7563T=JCM 33399T) is assigned as the type strain of a novel species within the genus Nocardioides, for which the name Nocardioidesyefusunii sp.
31511125	8	44	dep	C18 	1125:1128	arg1	 1ω9c					1130:1134	 1ω9c	1130:1134	 1ω9c	1130:1134	The predominant fatty acids (>10.0 %) were C18 : 1ω9c and iso-C16:0.
31511125	8	44	dep	C18 	1125:1128	arg1	iso-C16:0					1140:1148	iso-C16:0	1140:1148	iso-C16:0	1140:1148	The predominant fatty acids (>10.0 %) were C18 : 1ω9c and iso-C16:0.
31511125	9	45	theme	G+C	1159:1161	arg1	%					1222:1222	68.9 and 69.1 mol%	1205:1222	68.9 and 69.1 mol%	1205:1222	The DNA G+C contents of strains HY056T and HY057 were 68.9 and 69.1 mol%, respectively.
31511125	9	45	theme	G+C	1159:1161	arg1	contents					1163:1170	The DNA G+C contents	1151:1170	The DNA G+C contents of strains HY056T and HY057	1151:1198	The DNA G+C contents of strains HY056T and HY057 were 68.9 and 69.1 mol%, respectively.
31511125	10	46	theme	β-Glucosidase	1239:1251	arg1	expression					1253:1262	β-Glucosidase expression	1239:1262	β-Glucosidase expression	1239:1262	β-Glucosidase expression was positive, and acid was produced from d-fructose.
31511125	3	47	dep	97.0 	474:478	arg1	to					471:472	to	471:472	to	471:472	Phylogenetic analyses indicated that strains HY056T and HY057 belong to the genus Nocardioides by sharing a similarity ranging from 96.3 to 97.0 % in the 16S rRNA gene sequence and forming a distinct cluster with Nocardioides daphniae JCM 16608T, Nocardioides houyundeii 78T, Nocardioides solisilvae Ka25T and Nocardioides gilvus XZ17T.
31511125	6	48	theme	peptidoglycan	955:967	arg1	acid					923:926	ll-2,6-diaminopimelic acid	901:926	ll-2,6-diaminopimelic acid	901:926	The cell wall contained ll-2,6-diaminopimelic acid as the diamino acid of the peptidoglycan.
31511125	6	48	theme	peptidoglycan	955:967	arg1	acid					943:946	the diamino acid	931:946	the diamino acid of the peptidoglycan	931:967	The cell wall contained ll-2,6-diaminopimelic acid as the diamino acid of the peptidoglycan.
31511125	11	49	theme	Nocardioidesyefusunii	1459:1479	arg1	sp					1481:1482	the name Nocardioidesyefusunii sp	1450:1482	the name Nocardioidesyefusunii sp	1450:1482	Strain HY056T (=CGMCC 4.7563T=JCM 33399T) is assigned as the type strain of a novel species within the genus Nocardioides, for which the name Nocardioidesyefusunii sp.
31511125	9	50	theme	DNA	1155:1157	arg1	%					1222:1222	68.9 and 69.1 mol%	1205:1222	68.9 and 69.1 mol%	1205:1222	The DNA G+C contents of strains HY056T and HY057 were 68.9 and 69.1 mol%, respectively.
31511125	9	50	theme	DNA	1155:1157	arg1	contents					1163:1170	The DNA G+C contents	1151:1170	The DNA G+C contents of strains HY056T and HY057	1151:1198	The DNA G+C contents of strains HY056T and HY057 were 68.9 and 69.1 mol%, respectively.
31511125	8	51	theme	fatty	1098:1102	arg1	acids					1104:1108	The predominant fatty acids	1082:1108	The predominant fatty acids (>10.0 %)	1082:1118	The predominant fatty acids (>10.0 %) were C18 : 1ω9c and iso-C16:0.
31511125	8	51	theme	fatty	1098:1102	arg1	%					1117:1117	>10.0 %	1111:1117	>10.0 %	1111:1117	The predominant fatty acids (>10.0 %) were C18 : 1ω9c and iso-C16:0.
31511125	8	51	theme	fatty	1098:1102	arg1	C18 					1125:1128	C18 	1125:1128	C18 : 1ω9c and iso-C16:0	1125:1148	The predominant fatty acids (>10.0 %) were C18 : 1ω9c and iso-C16:0.
31511125	2	52	theme	different	270:278	arg1	regions					280:286	different regions	270:286	different regions	270:286	Two Gram-stain-positive, irregular rod-shaped (0.2-0.5×1.3-2.5 um) strains, HY056T and HY057, were isolated from the faeces of Equus kiang (the largest of the wild asses) collected at different regions from the Qinghai-Tibetan Plateau of PR China.
31511125	6	53	theme	cell	881:884	arg1	wall					886:889	The cell wall	877:889	The cell wall	877:889	The cell wall contained ll-2,6-diaminopimelic acid as the diamino acid of the peptidoglycan.
31511125	4	54	theme	HY056T	721:726	arg1	value					705:709	The digital DNA-DNA hybridization value	671:709	The digital DNA-DNA hybridization value of strain HY056T	671:726	The digital DNA-DNA hybridization value of strain HY056T was 96.9 % with strain HY057, but less than 30.0 % with the above four closest relatives.
31511125	4	54	theme	HY056T	721:726	arg1	%					737:737	96.9 %	732:737	96.9 % with strain HY057	732:755	The digital DNA-DNA hybridization value of strain HY056T was 96.9 % with strain HY057, but less than 30.0 % with the above four closest relatives.
31511125	9	55	theme	strains	1175:1181	arg1	%					1222:1222	68.9 and 69.1 mol%	1205:1222	68.9 and 69.1 mol%	1205:1222	The DNA G+C contents of strains HY056T and HY057 were 68.9 and 69.1 mol%, respectively.
31511125	9	55	theme	strains	1175:1181	arg1	contents					1163:1170	The DNA G+C contents	1151:1170	The DNA G+C contents of strains HY056T and HY057	1151:1198	The DNA G+C contents of strains HY056T and HY057 were 68.9 and 69.1 mol%, respectively.
31511125	0	56	dep	sp	23:24	arg1	Nocardioides					0:11	Nocardioides	0:11	Nocardioides	0:11	Nocardioides yefusunii sp.
31511125	4	57	theme	hybridization	691:703	arg1	value					705:709	The digital DNA-DNA hybridization value	671:709	The digital DNA-DNA hybridization value of strain HY056T	671:726	The digital DNA-DNA hybridization value of strain HY056T was 96.9 % with strain HY057, but less than 30.0 % with the above four closest relatives.
31511125	4	57	theme	hybridization	691:703	arg1	%					737:737	96.9 %	732:737	96.9 % with strain HY057	732:755	The digital DNA-DNA hybridization value of strain HY056T was 96.9 % with strain HY057, but less than 30.0 % with the above four closest relatives.
31511125	11	58	theme	novel	1395:1399	arg1	species					1401:1407	a novel species	1393:1407	a novel species	1393:1407	Strain HY056T (=CGMCC 4.7563T=JCM 33399T) is assigned as the type strain of a novel species within the genus Nocardioides, for which the name Nocardioidesyefusunii sp.
31511125	11	59	theme	type	1378:1381	arg1	strain					1383:1388	the type strain	1374:1388	the type strain of a novel species within the genus Nocardioides, for which the name Nocardioidesyefusunii sp	1374:1482	Strain HY056T (=CGMCC 4.7563T=JCM 33399T) is assigned as the type strain of a novel species within the genus Nocardioides, for which the name Nocardioidesyefusunii sp.
31511125	11	59	theme	type	1378:1381	arg1	HY056T					1324:1329	Strain HY056T	1317:1329	Strain HY056T (=CGMCC 4.7563T=JCM 33399T)	1317:1357	Strain HY056T (=CGMCC 4.7563T=JCM 33399T) is assigned as the type strain of a novel species within the genus Nocardioides, for which the name Nocardioidesyefusunii sp.
31511125	4	60	with	%	737:737	arg1	strain					744:749	strain HY057	744:755	strain HY057	744:755	The digital DNA-DNA hybridization value of strain HY056T was 96.9 % with strain HY057, but less than 30.0 % with the above four closest relatives.
31511125	11	61	theme	species	1401:1407	arg1	strain					1383:1388	the type strain	1374:1388	the type strain of a novel species within the genus Nocardioides, for which the name Nocardioidesyefusunii sp	1374:1482	Strain HY056T (=CGMCC 4.7563T=JCM 33399T) is assigned as the type strain of a novel species within the genus Nocardioides, for which the name Nocardioidesyefusunii sp.
31511125	11	61	theme	species	1401:1407	arg1	HY056T					1324:1329	Strain HY056T	1317:1329	Strain HY056T (=CGMCC 4.7563T=JCM 33399T)	1317:1357	Strain HY056T (=CGMCC 4.7563T=JCM 33399T) is assigned as the type strain of a novel species within the genus Nocardioides, for which the name Nocardioidesyefusunii sp.
31511125	6	62	theme	ll-2,6-diaminopimelic	901:921	arg1	acid					923:926	ll-2,6-diaminopimelic acid	901:926	ll-2,6-diaminopimelic acid	901:926	The cell wall contained ll-2,6-diaminopimelic acid as the diamino acid of the peptidoglycan.
31511125	6	62	theme	ll-2,6-diaminopimelic	901:921	arg1	acid					943:946	the diamino acid	931:946	the diamino acid of the peptidoglycan	931:967	The cell wall contained ll-2,6-diaminopimelic acid as the diamino acid of the peptidoglycan.
30767848	10	0	theme	novel	1302:1306	arg1	species					1308:1314	a novel species	1300:1314	a novel species	1300:1314	On the basis of the results obtained in this study, strain 3-5-3T is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacilluszei soli sp.
30767848	4	1	theme	96.5 	540:544	arg1	%					545:545	%	545:545	%	545:545	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 3-5-3T was affiliated with the genus Paenibacillus and most closely related to Paenibacillus anaericanus MH2T (96.5 % similarity).
30767848	5	2	theme	69.4-84.5 	687:696	arg1	%					713:713	69.4-84.5 % and 18.1-18.4 %	687:713	69.4-84.5 % and 18.1-18.4 %	687:713	The average nucleotide identity and digital DNA-DNA hybridization values between 3-5-3T and the closely related species ranged 69.4-84.5 % and 18.1-18.4 %.
30767848	9	3	theme	unidentified	1147:1158	arg1	phosphoaminolipid					1160:1176	one unidentified phosphoaminolipid	1143:1176	one unidentified phosphoaminolipid	1143:1176	The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, two unidentified glycolipids, two unidentified aminolipids, one unidentified phospholipid, one unidentified phosphoaminolipid and six unidentified lipids.
30767848	2	4	theme	Gram-stain-negative	110:128	arg1	strain					160:165	A Gram-stain-negative, rod-shaped, motile bacterial strain	108:165	A Gram-stain-negative, rod-shaped, motile bacterial strain	108:165	A Gram-stain-negative, rod-shaped, motile bacterial strain, designated 3-5-3T, was isolated from maize-cultivated soil artificially contaminated with cadmium, in Nanyang, Henan Province, China.
30767848	5	5	theme	%	697:697	arg1	%					713:713	69.4-84.5 % and 18.1-18.4 %	687:713	69.4-84.5 % and 18.1-18.4 %	687:713	The average nucleotide identity and digital DNA-DNA hybridization values between 3-5-3T and the closely related species ranged 69.4-84.5 % and 18.1-18.4 %.
30767848	1	6	theme	maize-cultivated	46:61	arg1	soil					63:66	maize-cultivated soil	46:66	maize-cultivated soil artificially contaminated with cadmium	46:105	nov., isolated from maize-cultivated soil artificially contaminated with cadmium.
30767848	8	7	theme	cell-wall	855:863	arg1	peptidoglycan					865:877	the cell-wall peptidoglycan	851:877	the cell-wall peptidoglycan	851:877	The diamino acid in the cell-wall peptidoglycan contains meso-diaminopimelic acid.
30767848	12	8	theme	1.13686T=KCTC	1441:1453	arg1	33998T					1455:1460	=CGMCC 1.13686T=KCTC 33998T	1434:1460	=CGMCC 1.13686T=KCTC 33998T	1434:1460	The type strain is 3-5-3T (=CGMCC 1.13686T=KCTC 33998T).
30767848	12	8	theme	1.13686T=KCTC	1441:1453	arg1	3-5-3T					1426:1431	3-5-3T	1426:1431	3-5-3T (=CGMCC 1.13686T=KCTC 33998T)	1426:1461	The type strain is 3-5-3T (=CGMCC 1.13686T=KCTC 33998T).
30767848	4	9	theme	related	497:503	arg1	MH2T					534:537	the genus Paenibacillus and most closely related to Paenibacillus anaericanus MH2T	456:537	the genus Paenibacillus and most closely related to Paenibacillus anaericanus MH2T (96.5 % similarity)	456:557	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 3-5-3T was affiliated with the genus Paenibacillus and most closely related to Paenibacillus anaericanus MH2T (96.5 % similarity).
30767848	4	9	theme	related	497:503	arg1	similarity					547:556	96.5 % similarity	540:556	96.5 % similarity	540:556	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 3-5-3T was affiliated with the genus Paenibacillus and most closely related to Paenibacillus anaericanus MH2T (96.5 % similarity).
30767848	5	10	theme	 and	698:701	arg1	%					713:713	69.4-84.5 % and 18.1-18.4 %	687:713	69.4-84.5 % and 18.1-18.4 %	687:713	The average nucleotide identity and digital DNA-DNA hybridization values between 3-5-3T and the closely related species ranged 69.4-84.5 % and 18.1-18.4 %.
30767848	6	11	theme	53.8 mol	744:751	arg1	G+C content					728:738	The genomic G+C content	716:738	The genomic G+C content	716:738	The genomic G+C content was 53.8 mol%.
30767848	6	11	theme	53.8 mol	744:751	arg1	%					752:752	53.8 mol%	744:752	53.8 mol%	744:752	The genomic G+C content was 53.8 mol%.
30767848	5	12	theme	hybridization	612:624	arg1	values					626:631	The average nucleotide identity and digital DNA-DNA hybridization values	560:631	The average nucleotide identity and digital DNA-DNA hybridization values between 3-5-3T and the closely related species	560:678	The average nucleotide identity and digital DNA-DNA hybridization values between 3-5-3T and the closely related species ranged 69.4-84.5 % and 18.1-18.4 %.
30767848	10	13	theme	soli	1380:1383	arg1	sp					1385:1386	the name Paenibacilluszei soli sp	1354:1386	the name Paenibacilluszei soli sp	1354:1386	On the basis of the results obtained in this study, strain 3-5-3T is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacilluszei soli sp.
30767848	4	14	theme	strain	422:427	arg1	3-5-3T					429:434	strain 3-5-3T	422:434	strain 3-5-3T	422:434	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 3-5-3T was affiliated with the genus Paenibacillus and most closely related to Paenibacillus anaericanus MH2T (96.5 % similarity).
30767848	10	15	theme	Paenibacilluszei	1363:1378	arg1	sp					1385:1386	the name Paenibacilluszei soli sp	1354:1386	the name Paenibacilluszei soli sp	1354:1386	On the basis of the results obtained in this study, strain 3-5-3T is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacilluszei soli sp.
30767848	2	16	theme	bacterial	150:158	arg1	strain					160:165	A Gram-stain-negative, rod-shaped, motile bacterial strain	108:165	A Gram-stain-negative, rod-shaped, motile bacterial strain	108:165	A Gram-stain-negative, rod-shaped, motile bacterial strain, designated 3-5-3T, was isolated from maize-cultivated soil artificially contaminated with cadmium, in Nanyang, Henan Province, China.
30767848	4	17	theme	rRNA	387:390	arg1	sequences					397:405	16S rRNA gene sequences	383:405	16S rRNA gene sequences	383:405	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 3-5-3T was affiliated with the genus Paenibacillus and most closely related to Paenibacillus anaericanus MH2T (96.5 % similarity).
30767848	9	18	contain	contained	938:946	arg2	aminolipids					1099:1109	two unidentified aminolipids	1082:1109	two unidentified aminolipids	1082:1109	The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, two unidentified glycolipids, two unidentified aminolipids, one unidentified phospholipid, one unidentified phosphoaminolipid and six unidentified lipids.
30767848	9	18	contain	contained	938:946	arg2	lipids					1199:1204	six unidentified lipids	1182:1204	six unidentified lipids	1182:1204	The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, two unidentified glycolipids, two unidentified aminolipids, one unidentified phospholipid, one unidentified phosphoaminolipid and six unidentified lipids.
30767848	9	18	contain	contained	938:946	arg2	diphosphatidylglycerol					948:969	diphosphatidylglycerol	948:969	diphosphatidylglycerol	948:969	The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, two unidentified glycolipids, two unidentified aminolipids, one unidentified phospholipid, one unidentified phosphoaminolipid and six unidentified lipids.
30767848	9	18	contain	contained	938:946	arg1	profile					930:936	The polar lipid profile	914:936	The polar lipid profile	914:936	The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, two unidentified glycolipids, two unidentified aminolipids, one unidentified phospholipid, one unidentified phosphoaminolipid and six unidentified lipids.
30767848	9	18	contain	contained	938:946	arg2	phosphoaminolipid					1160:1176	one unidentified phosphoaminolipid	1143:1176	one unidentified phosphoaminolipid	1143:1176	The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, two unidentified glycolipids, two unidentified aminolipids, one unidentified phospholipid, one unidentified phosphoaminolipid and six unidentified lipids.
30767848	9	18	contain	contained	938:946	arg2	phosphatidylglycerol					972:991	phosphatidylglycerol	972:991	phosphatidylglycerol	972:991	The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, two unidentified glycolipids, two unidentified aminolipids, one unidentified phospholipid, one unidentified phosphoaminolipid and six unidentified lipids.
30767848	9	18	contain	contained	938:946	arg2	phosphatidylethanolamine					994:1017	phosphatidylethanolamine	994:1017	phosphatidylethanolamine	994:1017	The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, two unidentified glycolipids, two unidentified aminolipids, one unidentified phospholipid, one unidentified phosphoaminolipid and six unidentified lipids.
30767848	9	18	contain	contained	938:946	arg2	glycolipids					1069:1079	two unidentified glycolipids	1052:1079	two unidentified glycolipids	1052:1079	The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, two unidentified glycolipids, two unidentified aminolipids, one unidentified phospholipid, one unidentified phosphoaminolipid and six unidentified lipids.
30767848	9	18	contain	contained	938:946	arg2	phosphatidylmethylethanolamine					1020:1049	phosphatidylmethylethanolamine	1020:1049	phosphatidylmethylethanolamine	1020:1049	The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, two unidentified glycolipids, two unidentified aminolipids, one unidentified phospholipid, one unidentified phosphoaminolipid and six unidentified lipids.
30767848	9	18	contain	contained	938:946	arg2	phospholipid					1129:1140	one unidentified phospholipid	1112:1140	one unidentified phospholipid	1112:1140	The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, two unidentified glycolipids, two unidentified aminolipids, one unidentified phospholipid, one unidentified phosphoaminolipid and six unidentified lipids.
30767848	9	19	theme	unidentified	1186:1197	arg1	lipids					1199:1204	six unidentified lipids	1182:1204	six unidentified lipids	1182:1204	The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, two unidentified glycolipids, two unidentified aminolipids, one unidentified phospholipid, one unidentified phosphoaminolipid and six unidentified lipids.
30767848	10	20	dep	results	1227:1233	arg1	the					1210:1212	the	1210:1212	the	1210:1212	On the basis of the results obtained in this study, strain 3-5-3T is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacilluszei soli sp.
30767848	10	20	dep	results	1227:1233	arg1	basis					1214:1218	basis	1214:1218	basis	1214:1218	On the basis of the results obtained in this study, strain 3-5-3T is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacilluszei soli sp.
30767848	5	21	theme	related	664:670	arg1	species					672:678	the closely related species	652:678	the closely related species	652:678	The average nucleotide identity and digital DNA-DNA hybridization values between 3-5-3T and the closely related species ranged 69.4-84.5 % and 18.1-18.4 %.
30767848	4	22	theme	16S	383:385	arg1	sequences					397:405	16S rRNA gene sequences	383:405	16S rRNA gene sequences	383:405	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 3-5-3T was affiliated with the genus Paenibacillus and most closely related to Paenibacillus anaericanus MH2T (96.5 % similarity).
30767848	5	23	theme	average	564:570	arg1	identity					583:590	average nucleotide identity	564:590	average nucleotide identity	564:590	The average nucleotide identity and digital DNA-DNA hybridization values between 3-5-3T and the closely related species ranged 69.4-84.5 % and 18.1-18.4 %.
30767848	6	24	theme	genomic	720:726	arg1	G+C content					728:738	The genomic G+C content	716:738	The genomic G+C content	716:738	The genomic G+C content was 53.8 mol%.
30767848	6	24	theme	genomic	720:726	arg1	%					752:752	53.8 mol%	744:752	53.8 mol%	744:752	The genomic G+C content was 53.8 mol%.
30767848	2	25	attach	isolated	191:198	arg2	strain					160:165	A Gram-stain-negative, rod-shaped, motile bacterial strain	108:165	A Gram-stain-negative, rod-shaped, motile bacterial strain	108:165	A Gram-stain-negative, rod-shaped, motile bacterial strain, designated 3-5-3T, was isolated from maize-cultivated soil artificially contaminated with cadmium, in Nanyang, Henan Province, China.
30767848	2	25	attach	isolated	191:198	arg1	soil					222:225	maize-cultivated soil	205:225	maize-cultivated soil artificially contaminated with cadmium	205:264	A Gram-stain-negative, rod-shaped, motile bacterial strain, designated 3-5-3T, was isolated from maize-cultivated soil artificially contaminated with cadmium, in Nanyang, Henan Province, China.
30767848	0	26	theme	Paenibacillus	0:12	arg1	zeisoli					14:20	Paenibacillus zeisoli	0:20	Paenibacillus zeisoli	0:20	Paenibacillus zeisoli sp.
30767848	7	27	theme	only	813:816	arg1	Anteiso-C15 					755:766	Anteiso-C15 	755:766	Anteiso-C15 : 0 was the major fatty acid and MK-7 was the only menaquinone.	755:829	Anteiso-C15 : 0 was the major fatty acid and MK-7 was the only menaquinone.
30767848	7	27	theme	only	813:816	arg1	MK-7					800:803	MK-7	800:803	MK-7	800:803	Anteiso-C15 : 0 was the major fatty acid and MK-7 was the only menaquinone.
30767848	7	27	theme	only	813:816	arg1	menaquinone					818:828	the only menaquinone	809:828	the only menaquinone	809:828	Anteiso-C15 : 0 was the major fatty acid and MK-7 was the only menaquinone.
30767848	5	28	theme	DNA-DNA	604:610	arg1	hybridization					612:624	digital DNA-DNA hybridization	596:624	digital DNA-DNA hybridization	596:624	The average nucleotide identity and digital DNA-DNA hybridization values between 3-5-3T and the closely related species ranged 69.4-84.5 % and 18.1-18.4 %.
30767848	12	29	theme	=CGMCC	1434:1439	arg1	33998T					1455:1460	=CGMCC 1.13686T=KCTC 33998T	1434:1460	=CGMCC 1.13686T=KCTC 33998T	1434:1460	The type strain is 3-5-3T (=CGMCC 1.13686T=KCTC 33998T).
30767848	12	29	theme	=CGMCC	1434:1439	arg1	3-5-3T					1426:1431	3-5-3T	1426:1431	3-5-3T (=CGMCC 1.13686T=KCTC 33998T)	1426:1461	The type strain is 3-5-3T (=CGMCC 1.13686T=KCTC 33998T).
30767848	9	30	theme	unidentified	1056:1067	arg1	glycolipids					1069:1079	two unidentified glycolipids	1052:1079	two unidentified glycolipids	1052:1079	The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, two unidentified glycolipids, two unidentified aminolipids, one unidentified phospholipid, one unidentified phosphoaminolipid and six unidentified lipids.
30767848	9	30	theme	unidentified	1056:1067	arg1	aminolipids					1099:1109	two unidentified aminolipids	1082:1109	two unidentified aminolipids	1082:1109	The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, two unidentified glycolipids, two unidentified aminolipids, one unidentified phospholipid, one unidentified phosphoaminolipid and six unidentified lipids.
30767848	4	31	theme	Paenibacillus	508:520	arg1	anaericanus					522:532	Paenibacillus anaericanus	508:532	Paenibacillus anaericanus	508:532	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 3-5-3T was affiliated with the genus Paenibacillus and most closely related to Paenibacillus anaericanus MH2T (96.5 % similarity).
30767848	4	32	theme	Paenibacillus	466:478	arg1	MH2T					534:537	the genus Paenibacillus and most closely related to Paenibacillus anaericanus MH2T	456:537	the genus Paenibacillus and most closely related to Paenibacillus anaericanus MH2T (96.5 % similarity)	456:557	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 3-5-3T was affiliated with the genus Paenibacillus and most closely related to Paenibacillus anaericanus MH2T (96.5 % similarity).
30767848	4	32	theme	Paenibacillus	466:478	arg1	similarity					547:556	96.5 % similarity	540:556	96.5 % similarity	540:556	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 3-5-3T was affiliated with the genus Paenibacillus and most closely related to Paenibacillus anaericanus MH2T (96.5 % similarity).
30767848	8	33	from	acid	843:846	arg1	peptidoglycan					865:877	the cell-wall peptidoglycan	851:877	the cell-wall peptidoglycan	851:877	The diamino acid in the cell-wall peptidoglycan contains meso-diaminopimelic acid.
30767848	4	34	theme	genus	460:464	arg1	MH2T					534:537	the genus Paenibacillus and most closely related to Paenibacillus anaericanus MH2T	456:537	the genus Paenibacillus and most closely related to Paenibacillus anaericanus MH2T (96.5 % similarity)	456:557	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 3-5-3T was affiliated with the genus Paenibacillus and most closely related to Paenibacillus anaericanus MH2T (96.5 % similarity).
30767848	4	34	theme	genus	460:464	arg1	similarity					547:556	96.5 % similarity	540:556	96.5 % similarity	540:556	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 3-5-3T was affiliated with the genus Paenibacillus and most closely related to Paenibacillus anaericanus MH2T (96.5 % similarity).
30767848	10	35	theme	name	1358:1361	arg1	sp					1385:1386	the name Paenibacilluszei soli sp	1354:1386	the name Paenibacilluszei soli sp	1354:1386	On the basis of the results obtained in this study, strain 3-5-3T is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacilluszei soli sp.
30767848	9	36	theme	unidentified	1086:1097	arg1	glycolipids					1069:1079	two unidentified glycolipids	1052:1079	two unidentified glycolipids	1052:1079	The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, two unidentified glycolipids, two unidentified aminolipids, one unidentified phospholipid, one unidentified phosphoaminolipid and six unidentified lipids.
30767848	9	36	theme	unidentified	1086:1097	arg1	aminolipids					1099:1109	two unidentified aminolipids	1082:1109	two unidentified aminolipids	1082:1109	The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, two unidentified glycolipids, two unidentified aminolipids, one unidentified phospholipid, one unidentified phosphoaminolipid and six unidentified lipids.
30767848	4	37	theme	%	545:545	arg1	MH2T					534:537	the genus Paenibacillus and most closely related to Paenibacillus anaericanus MH2T	456:537	the genus Paenibacillus and most closely related to Paenibacillus anaericanus MH2T (96.5 % similarity)	456:557	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 3-5-3T was affiliated with the genus Paenibacillus and most closely related to Paenibacillus anaericanus MH2T (96.5 % similarity).
30767848	4	37	theme	%	545:545	arg1	similarity					547:556	96.5 % similarity	540:556	96.5 % similarity	540:556	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 3-5-3T was affiliated with the genus Paenibacillus and most closely related to Paenibacillus anaericanus MH2T (96.5 % similarity).
30767848	4	38	theme	Phylogenetic	352:363	arg1	analysis					365:372	Phylogenetic analysis	352:372	Phylogenetic analysis based on 16S rRNA gene sequences	352:405	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 3-5-3T was affiliated with the genus Paenibacillus and most closely related to Paenibacillus anaericanus MH2T (96.5 % similarity).
30767848	7	39	theme	fatty	785:789	arg1	Anteiso-C15 					755:766	Anteiso-C15 	755:766	Anteiso-C15 : 0 was the major fatty acid and MK-7 was the only menaquinone.	755:829	Anteiso-C15 : 0 was the major fatty acid and MK-7 was the only menaquinone.
30767848	7	39	theme	fatty	785:789	arg1	acid					791:794	the major fatty acid	775:794	the major fatty acid	775:794	Anteiso-C15 : 0 was the major fatty acid and MK-7 was the only menaquinone.
30767848	7	39	theme	fatty	785:789	arg1	 0					768:769	 0	768:769	 0	768:769	Anteiso-C15 : 0 was the major fatty acid and MK-7 was the only menaquinone.
30767848	9	40	theme	polar	918:922	arg1	profile					930:936	The polar lipid profile	914:936	The polar lipid profile	914:936	The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, two unidentified glycolipids, two unidentified aminolipids, one unidentified phospholipid, one unidentified phosphoaminolipid and six unidentified lipids.
30767848	5	41	theme	nucleotide	572:581	arg1	identity					583:590	average nucleotide identity	564:590	average nucleotide identity	564:590	The average nucleotide identity and digital DNA-DNA hybridization values between 3-5-3T and the closely related species ranged 69.4-84.5 % and 18.1-18.4 %.
30767848	10	42	theme	genus	1323:1327	arg1	species					1308:1314	a novel species	1300:1314	a novel species	1300:1314	On the basis of the results obtained in this study, strain 3-5-3T is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacilluszei soli sp.
30767848	4	43	theme	gene	392:395	arg1	sequences					397:405	16S rRNA gene sequences	383:405	16S rRNA gene sequences	383:405	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 3-5-3T was affiliated with the genus Paenibacillus and most closely related to Paenibacillus anaericanus MH2T (96.5 % similarity).
30767848	9	44	theme	lipid	924:928	arg1	profile					930:936	The polar lipid profile	914:936	The polar lipid profile	914:936	The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, two unidentified glycolipids, two unidentified aminolipids, one unidentified phospholipid, one unidentified phosphoaminolipid and six unidentified lipids.
30767848	2	45	theme	maize-cultivated	205:220	arg1	soil					222:225	maize-cultivated soil	205:225	maize-cultivated soil artificially contaminated with cadmium	205:264	A Gram-stain-negative, rod-shaped, motile bacterial strain, designated 3-5-3T, was isolated from maize-cultivated soil artificially contaminated with cadmium, in Nanyang, Henan Province, China.
30767848	5	46	theme	identity	583:590	arg1	values					626:631	The average nucleotide identity and digital DNA-DNA hybridization values	560:631	The average nucleotide identity and digital DNA-DNA hybridization values between 3-5-3T and the closely related species	560:678	The average nucleotide identity and digital DNA-DNA hybridization values between 3-5-3T and the closely related species ranged 69.4-84.5 % and 18.1-18.4 %.
30767848	5	47	theme	digital	596:602	arg1	hybridization					612:624	digital DNA-DNA hybridization	596:624	digital DNA-DNA hybridization	596:624	The average nucleotide identity and digital DNA-DNA hybridization values between 3-5-3T and the closely related species ranged 69.4-84.5 % and 18.1-18.4 %.
30767848	3	48	theme	Strain	302:307	arg1	3-5-3T					309:314	Strain 3-5-3T	302:314	Strain 3-5-3T	302:314	Strain 3-5-3T was oxidase- and catalase-positive.
30767848	0	49	dep	sp	22:23	arg1	zeisoli					14:20	Paenibacillus zeisoli	0:20	Paenibacillus zeisoli	0:20	Paenibacillus zeisoli sp.
30767848	9	50	theme	unidentified	1116:1127	arg1	phospholipid					1129:1140	one unidentified phospholipid	1112:1140	one unidentified phospholipid	1112:1140	The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, two unidentified glycolipids, two unidentified aminolipids, one unidentified phospholipid, one unidentified phosphoaminolipid and six unidentified lipids.
30767848	7	51	theme	major	779:783	arg1	Anteiso-C15 					755:766	Anteiso-C15 	755:766	Anteiso-C15 : 0 was the major fatty acid and MK-7 was the only menaquinone.	755:829	Anteiso-C15 : 0 was the major fatty acid and MK-7 was the only menaquinone.
30767848	7	51	theme	major	779:783	arg1	acid					791:794	the major fatty acid	775:794	the major fatty acid	775:794	Anteiso-C15 : 0 was the major fatty acid and MK-7 was the only menaquinone.
30767848	7	51	theme	major	779:783	arg1	 0					768:769	 0	768:769	 0	768:769	Anteiso-C15 : 0 was the major fatty acid and MK-7 was the only menaquinone.
30767848	8	52	contain	contains	879:886	arg1	acid					843:846	The diamino acid	831:846	The diamino acid in the cell-wall peptidoglycan	831:877	The diamino acid in the cell-wall peptidoglycan contains meso-diaminopimelic acid.
30767848	8	52	contain	contains	879:886	arg2	acid					908:911	meso-diaminopimelic acid	888:911	meso-diaminopimelic acid	888:911	The diamino acid in the cell-wall peptidoglycan contains meso-diaminopimelic acid.
30767848	10	53	theme	Paenibacillus	1329:1341	arg1	genus					1323:1327	the genus Paenibacillus	1319:1341	the genus Paenibacillus	1319:1341	On the basis of the results obtained in this study, strain 3-5-3T is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacilluszei soli sp.
30767848	12	54	theme	type	1411:1414	arg1	3-5-3T					1426:1431	3-5-3T	1426:1431	3-5-3T (=CGMCC 1.13686T=KCTC 33998T)	1426:1461	The type strain is 3-5-3T (=CGMCC 1.13686T=KCTC 33998T).
30767848	12	54	theme	type	1411:1414	arg1	strain					1416:1421	The type strain	1407:1421	The type strain	1407:1421	The type strain is 3-5-3T (=CGMCC 1.13686T=KCTC 33998T).
30767848	8	55	theme	meso-diaminopimelic	888:906	arg1	acid					908:911	meso-diaminopimelic acid	888:911	meso-diaminopimelic acid	888:911	The diamino acid in the cell-wall peptidoglycan contains meso-diaminopimelic acid.
30767848	8	56	theme	diamino	835:841	arg1	acid					843:846	The diamino acid	831:846	The diamino acid in the cell-wall peptidoglycan	831:877	The diamino acid in the cell-wall peptidoglycan contains meso-diaminopimelic acid.
30767848	10	57	theme	strain	1259:1264	arg1	3-5-3T					1266:1271	strain 3-5-3T	1259:1271	strain 3-5-3T	1259:1271	On the basis of the results obtained in this study, strain 3-5-3T is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacilluszei soli sp.
30767848	2	58	dep	Gram-stain-negative	110:128	arg1	rod-shaped					131:140	rod-shaped	131:140	rod-shaped	131:140	A Gram-stain-negative, rod-shaped, motile bacterial strain, designated 3-5-3T, was isolated from maize-cultivated soil artificially contaminated with cadmium, in Nanyang, Henan Province, China.
30767848	2	58	dep	Gram-stain-negative	110:128	arg1	motile					143:148	motile	143:148	motile	143:148	A Gram-stain-negative, rod-shaped, motile bacterial strain, designated 3-5-3T, was isolated from maize-cultivated soil artificially contaminated with cadmium, in Nanyang, Henan Province, China.
30711608	9	0	theme	small	1544:1548	arg1	clusters					1550:1557	small clusters	1544:1557	small clusters for perlecan (p = 0.008)	1544:1582	The earliest point with a significant reduction in protein content as shown by ELISA was the transition from single strings to small clusters for collagen type VI (p = 0.016) and from double strings to small clusters for perlecan (p = 0.008), with the lowest amounts for both proteins seen in big clusters.
30711608	7	1	theme	single	1074:1079	arg1	p = 0.030					1100:1108	p = 0.030	1100:1108	p = 0.030	1100:1108	RESULTS PCM stiffness significantly decreased with the development from single to double strings (p = 0.030), from double strings to small clusters (p = 0.015), and from small clusters to big clusters (p < 0.001).
30711608	7	1	theme	single	1074:1079	arg1	strings					1091:1097	single to double strings	1074:1097	single to double strings (p = 0.030)	1074:1109	RESULTS PCM stiffness significantly decreased with the development from single to double strings (p = 0.030), from double strings to small clusters (p = 0.015), and from small clusters to big clusters (p < 0.001).
30711608	4	2	theme	predominant	642:652	arg1	pattern					671:677	their predominant spatial cellular pattern	636:677	their predominant spatial cellular pattern	636:677	METHOD Cartilage samples were selected according to their predominant spatial cellular pattern.
30711608	9	3	dep	type	1497:1500	arg1	VI					1502:1503	VI	1502:1503	VI	1502:1503	The earliest point with a significant reduction in protein content as shown by ELISA was the transition from single strings to small clusters for collagen type VI (p = 0.016) and from double strings to small clusters for perlecan (p = 0.008), with the lowest amounts for both proteins seen in big clusters.
30711608	7	4	dep	single	1074:1079	arg1	to					1081:1082	to	1081:1082	to	1081:1082	RESULTS PCM stiffness significantly decreased with the development from single to double strings (p = 0.030), from double strings to small clusters (p = 0.015), and from small clusters to big clusters (p < 0.001).
30711608	7	4	dep	single	1074:1079	arg1	double					1084:1089	double	1084:1089	double	1084:1089	RESULTS PCM stiffness significantly decreased with the development from single to double strings (p = 0.030), from double strings to small clusters (p = 0.015), and from small clusters to big clusters (p < 0.001).
30711608	3	5	theme	spatial	539:545	arg1	organisation					547:558	spatial organisation	539:558	spatial organisation	539:558	The aim of this study was to establish the functional relevance of spatial organisation in articular cartilage.
30711608	0	6	theme	organisation	113:124	arg1	function					81:88	a function	79:88	a function of spatial chondrocyte organisation in osteoarthritic cartilage	79:152	Changes in stiffness and biochemical composition of the pericellular matrix as a function of spatial chondrocyte organisation in osteoarthritic cartilage.
30711608	5	7	theme	PCM	705:707	arg1	modulus					688:694	Young's modulus	680:694	Young's modulus of their PCM	680:707	Young's modulus of their PCM was measured by atomic force microscopy (AFM) (∼500 measurements/pattern).
30711608	9	8	theme	protein	1393:1399	arg1	content					1401:1407	protein content	1393:1407	protein content as shown by ELISA	1393:1425	The earliest point with a significant reduction in protein content as shown by ELISA was the transition from single strings to small clusters for collagen type VI (p = 0.016) and from double strings to small clusters for perlecan (p = 0.008), with the lowest amounts for both proteins seen in big clusters.
30711608	9	9	theme	lowest	1594:1599	arg1	amounts					1601:1607	the lowest amounts	1590:1607	the lowest amounts for both proteins seen in big clusters	1590:1646	The earliest point with a significant reduction in protein content as shown by ELISA was the transition from single strings to small clusters for collagen type VI (p = 0.016) and from double strings to small clusters for perlecan (p = 0.008), with the lowest amounts for both proteins seen in big clusters.
30711608	3	10	from	relevance	526:534	arg1	cartilage					573:581	articular cartilage	563:581	articular cartilage	563:581	The aim of this study was to establish the functional relevance of spatial organisation in articular cartilage.
30711608	10	11	theme	spatial	1713:1719	arg1	organisation					1733:1744	spatial chondrocyte organisation	1713:1744	spatial chondrocyte organisation	1713:1744	CONCLUSIONS This study demonstrates the functional relevance of spatial chondrocyte organisation as an image-based biomarker.
30711608	0	12	from	function	81:88	arg1	cartilage					144:152	osteoarthritic cartilage	129:152	osteoarthritic cartilage	129:152	Changes in stiffness and biochemical composition of the pericellular matrix as a function of spatial chondrocyte organisation in osteoarthritic cartilage.
30711608	0	13	theme	pericellular	56:67	arg1	matrix					69:74	the pericellular matrix	52:74	the pericellular matrix	52:74	Changes in stiffness and biochemical composition of the pericellular matrix as a function of spatial chondrocyte organisation in osteoarthritic cartilage.
30711608	9	14	from	strings	1458:1464	arg1	point					1355:1359	The earliest point	1342:1359	The earliest point with a significant reduction in protein content as shown by ELISA	1342:1425	The earliest point with a significant reduction in protein content as shown by ELISA was the transition from single strings to small clusters for collagen type VI (p = 0.016) and from double strings to small clusters for perlecan (p = 0.008), with the lowest amounts for both proteins seen in big clusters.
30711608	9	14	from	strings	1458:1464	arg1	transition					1435:1444	the transition	1431:1444	the transition from single strings to small clusters for collagen type VI (p = 0.016) and from double strings to small clusters for perlecan (p = 0.008)	1431:1582	The earliest point with a significant reduction in protein content as shown by ELISA was the transition from single strings to small clusters for collagen type VI (p = 0.016) and from double strings to small clusters for perlecan (p = 0.008), with the lowest amounts for both proteins seen in big clusters.
30711608	5	15	theme	atomic	725:730	arg1	microscopy					738:747	atomic force microscopy	725:747	atomic force microscopy (AFM) (∼500 measurements/pattern)	725:781	Young's modulus of their PCM was measured by atomic force microscopy (AFM) (∼500 measurements/pattern).
30711608	5	15	theme	atomic	725:730	arg1	measurements/pattern					761:780	∼500 measurements/pattern	756:780	∼500 measurements/pattern	756:780	Young's modulus of their PCM was measured by atomic force microscopy (AFM) (∼500 measurements/pattern).
30711608	5	15	theme	atomic	725:730	arg1	AFM					750:752	AFM	750:752	AFM	750:752	Young's modulus of their PCM was measured by atomic force microscopy (AFM) (∼500 measurements/pattern).
30711608	6	16	theme	immunosorbent	959:971	arg1	ELISA					980:984	ELISA	980:984	ELISA	980:984	The distribution of two major PCM components (collagen type VI and perlecan) was analysed by immunohistochemistry (8 patients) and protein content quantified by enzyme-linked immunosorbent assay (ELISA) (58 patients).
30711608	6	16	theme	immunosorbent	959:971	arg1	assay					973:977	enzyme-linked immunosorbent assay	945:977	enzyme-linked immunosorbent assay (ELISA) (58 patients)	945:999	The distribution of two major PCM components (collagen type VI and perlecan) was analysed by immunohistochemistry (8 patients) and protein content quantified by enzyme-linked immunosorbent assay (ELISA) (58 patients).
30711608	6	16	theme	immunosorbent	959:971	arg1	patients					991:998	58 patients	988:998	58 patients	988:998	The distribution of two major PCM components (collagen type VI and perlecan) was analysed by immunohistochemistry (8 patients) and protein content quantified by enzyme-linked immunosorbent assay (ELISA) (58 patients).
30711608	0	17	from	Changes	0:6	arg1	composition					37:47	stiffness and biochemical composition	11:47	stiffness and biochemical composition of the pericellular matrix	11:74	Changes in stiffness and biochemical composition of the pericellular matrix as a function of spatial chondrocyte organisation in osteoarthritic cartilage.
30711608	8	18	theme	compact	1248:1254	arg1	focalised					1331:1339	focalised	1331:1339	focalised	1331:1339	At the same time, the initially compact collagen type VI and perlecan staining progressively weakened and was less focalised.
30711608	8	18	theme	compact	1248:1254	arg1	type					1265:1268	the initially compact collagen type	1234:1268	the initially compact collagen type	1234:1268	At the same time, the initially compact collagen type VI and perlecan staining progressively weakened and was less focalised.
30711608	1	19	dep	OBJECTIVE	155:163	arg1	seem					206:209	seem	206:209	seem to change their spatial arrangement from single to double strings, small and big clusters	206:299	OBJECTIVE During osteoarthritis (OA), chondrocytes seem to change their spatial arrangement from single to double strings, small and big clusters.
30711608	9	20	theme	single	1451:1456	arg1	strings					1458:1464	single strings	1451:1464	single strings to small clusters for collagen type VI (p = 0.016)	1451:1515	The earliest point with a significant reduction in protein content as shown by ELISA was the transition from single strings to small clusters for collagen type VI (p = 0.016) and from double strings to small clusters for perlecan (p = 0.008), with the lowest amounts for both proteins seen in big clusters.
30711608	7	21	theme	small	1135:1139	arg1	clusters					1141:1148	small clusters	1135:1148	small clusters (p = 0.015)	1135:1160	RESULTS PCM stiffness significantly decreased with the development from single to double strings (p = 0.030), from double strings to small clusters (p = 0.015), and from small clusters to big clusters (p < 0.001).
30711608	7	21	theme	small	1135:1139	arg1	p = 0.015					1151:1159	p = 0.015	1151:1159	p = 0.015	1151:1159	RESULTS PCM stiffness significantly decreased with the development from single to double strings (p = 0.030), from double strings to small clusters (p = 0.015), and from small clusters to big clusters (p < 0.001).
30711608	7	22	from	strings	1124:1130	arg1	development					1057:1067	the development	1053:1067	the development from single to double strings (p = 0.030), from double strings to small clusters (p = 0.015), and from small clusters to big clusters (p < 0.001)	1053:1213	RESULTS PCM stiffness significantly decreased with the development from single to double strings (p = 0.030), from double strings to small clusters (p = 0.015), and from small clusters to big clusters (p < 0.001).
30711608	0	23	theme	matrix	69:74	arg1	composition					37:47	stiffness and biochemical composition	11:47	stiffness and biochemical composition of the pericellular matrix	11:74	Changes in stiffness and biochemical composition of the pericellular matrix as a function of spatial chondrocyte organisation in osteoarthritic cartilage.
30711608	2	24	theme	spatial	415:421	arg1	patterns					423:430	spatial patterns	415:430	spatial patterns	415:430	Since the pericellular matrix (PCM) appears to degrade alongside this reorganisation, it has been suggested that spatial patterns act as an image-based biomarker for OA.
30711608	2	24	theme	spatial	415:421	arg1	biomarker					454:462	an image-based biomarker	439:462	an image-based biomarker for OA	439:469	Since the pericellular matrix (PCM) appears to degrade alongside this reorganisation, it has been suggested that spatial patterns act as an image-based biomarker for OA.
30711608	11	25	dep	decreases	1837:1845	arg1	followed					1848:1855	followed	1848:1855	followed by protein degradation from double strings to small clusters	1848:1916	At the transition from single to double strings PCM stiffness decreases, followed by protein degradation from double strings to small clusters.
30711608	11	26	theme	PCM	1823:1825	arg1	stiffness					1827:1835	PCM stiffness	1823:1835	PCM stiffness	1823:1835	At the transition from single to double strings PCM stiffness decreases, followed by protein degradation from double strings to small clusters.
30711608	0	27	theme	stiffness	11:19	arg1	composition					37:47	stiffness and biochemical composition	11:47	stiffness and biochemical composition of the pericellular matrix	11:74	Changes in stiffness and biochemical composition of the pericellular matrix as a function of spatial chondrocyte organisation in osteoarthritic cartilage.
30711608	9	28	theme	collagen	1488:1495	arg1	type					1497:1500	collagen type VI (p = 0.016)	1488:1515	collagen type VI (p = 0.016)	1488:1515	The earliest point with a significant reduction in protein content as shown by ELISA was the transition from single strings to small clusters for collagen type VI (p = 0.016) and from double strings to small clusters for perlecan (p = 0.008), with the lowest amounts for both proteins seen in big clusters.
30711608	11	29	theme	double	1808:1813	arg1	strings					1815:1821	double strings	1808:1821	double strings	1808:1821	At the transition from single to double strings PCM stiffness decreases, followed by protein degradation from double strings to small clusters.
30711608	0	30	theme	biochemical	25:35	arg1	composition					37:47	stiffness and biochemical composition	11:47	stiffness and biochemical composition of the pericellular matrix	11:74	Changes in stiffness and biochemical composition of the pericellular matrix as a function of spatial chondrocyte organisation in osteoarthritic cartilage.
30711608	6	31	dep	components	818:827	arg1	perlecan					851:858	perlecan	851:858	perlecan	851:858	The distribution of two major PCM components (collagen type VI and perlecan) was analysed by immunohistochemistry (8 patients) and protein content quantified by enzyme-linked immunosorbent assay (ELISA) (58 patients).
30711608	6	31	dep	components	818:827	arg1	components					818:827	two major PCM components	804:827	two major PCM components (collagen type VI and perlecan)	804:859	The distribution of two major PCM components (collagen type VI and perlecan) was analysed by immunohistochemistry (8 patients) and protein content quantified by enzyme-linked immunosorbent assay (ELISA) (58 patients).
30711608	6	31	dep	components	818:827	arg1	type					839:842	collagen type VI	830:845	collagen type VI	830:845	The distribution of two major PCM components (collagen type VI and perlecan) was analysed by immunohistochemistry (8 patients) and protein content quantified by enzyme-linked immunosorbent assay (ELISA) (58 patients).
30711608	10	32	theme	organisation	1733:1744	arg1	biomarker					1764:1772	an image-based biomarker	1749:1772	an image-based biomarker	1749:1772	CONCLUSIONS This study demonstrates the functional relevance of spatial chondrocyte organisation as an image-based biomarker.
30711608	10	32	theme	organisation	1733:1744	arg1	relevance					1700:1708	the functional relevance	1685:1708	the functional relevance of spatial chondrocyte organisation	1685:1744	CONCLUSIONS This study demonstrates the functional relevance of spatial chondrocyte organisation as an image-based biomarker.
30711608	6	33	theme	PCM	814:816	arg1	perlecan					851:858	perlecan	851:858	perlecan	851:858	The distribution of two major PCM components (collagen type VI and perlecan) was analysed by immunohistochemistry (8 patients) and protein content quantified by enzyme-linked immunosorbent assay (ELISA) (58 patients).
30711608	6	33	theme	PCM	814:816	arg1	components					818:827	two major PCM components	804:827	two major PCM components (collagen type VI and perlecan)	804:859	The distribution of two major PCM components (collagen type VI and perlecan) was analysed by immunohistochemistry (8 patients) and protein content quantified by enzyme-linked immunosorbent assay (ELISA) (58 patients).
30711608	6	33	theme	PCM	814:816	arg1	type					839:842	collagen type VI	830:845	collagen type VI	830:845	The distribution of two major PCM components (collagen type VI and perlecan) was analysed by immunohistochemistry (8 patients) and protein content quantified by enzyme-linked immunosorbent assay (ELISA) (58 patients).
30711608	9	34	theme	double	1526:1531	arg1	strings					1533:1539	double strings	1526:1539	double strings	1526:1539	The earliest point with a significant reduction in protein content as shown by ELISA was the transition from single strings to small clusters for collagen type VI (p = 0.016) and from double strings to small clusters for perlecan (p = 0.008), with the lowest amounts for both proteins seen in big clusters.
30711608	11	35	from	strings	1892:1898	arg1	degradation					1868:1878	protein degradation	1860:1878	protein degradation from double strings to small clusters	1860:1916	At the transition from single to double strings PCM stiffness decreases, followed by protein degradation from double strings to small clusters.
30711608	4	36	theme	Cartilage	591:599	arg1	samples					601:607	METHOD Cartilage samples	584:607	METHOD Cartilage samples	584:607	METHOD Cartilage samples were selected according to their predominant spatial cellular pattern.
30711608	1	37	theme	single	252:257	arg1	clusters					292:299	single to double strings, small and big clusters	252:299	single to double strings, small and big clusters	252:299	OBJECTIVE During osteoarthritis (OA), chondrocytes seem to change their spatial arrangement from single to double strings, small and big clusters.
30711608	7	38	theme	small	1172:1176	arg1	clusters					1178:1185	small clusters	1172:1185	small clusters	1172:1185	RESULTS PCM stiffness significantly decreased with the development from single to double strings (p = 0.030), from double strings to small clusters (p = 0.015), and from small clusters to big clusters (p < 0.001).
30711608	1	39	from	clusters	292:299	arg1	arrangement					235:245	their spatial arrangement	221:245	their spatial arrangement from single to double strings, small and big clusters	221:299	OBJECTIVE During osteoarthritis (OA), chondrocytes seem to change their spatial arrangement from single to double strings, small and big clusters.
30711608	11	40	theme	small	1903:1907	arg1	clusters					1909:1916	small clusters	1903:1916	small clusters	1903:1916	At the transition from single to double strings PCM stiffness decreases, followed by protein degradation from double strings to small clusters.
30711608	1	41	theme	double	262:267	arg1	strings					269:275	double strings	262:275	double strings	262:275	OBJECTIVE During osteoarthritis (OA), chondrocytes seem to change their spatial arrangement from single to double strings, small and big clusters.
30711608	7	42	from	clusters	1178:1185	arg1	development					1057:1067	the development	1053:1067	the development from single to double strings (p = 0.030), from double strings to small clusters (p = 0.015), and from small clusters to big clusters (p < 0.001)	1053:1213	RESULTS PCM stiffness significantly decreased with the development from single to double strings (p = 0.030), from double strings to small clusters (p = 0.015), and from small clusters to big clusters (p < 0.001).
30711608	4	43	theme	cellular	662:669	arg1	pattern					671:677	their predominant spatial cellular pattern	636:677	their predominant spatial cellular pattern	636:677	METHOD Cartilage samples were selected according to their predominant spatial cellular pattern.
30711608	9	44	theme	earliest	1346:1353	arg1	point					1355:1359	The earliest point	1342:1359	The earliest point with a significant reduction in protein content as shown by ELISA	1342:1425	The earliest point with a significant reduction in protein content as shown by ELISA was the transition from single strings to small clusters for collagen type VI (p = 0.016) and from double strings to small clusters for perlecan (p = 0.008), with the lowest amounts for both proteins seen in big clusters.
30711608	9	44	theme	earliest	1346:1353	arg1	transition					1435:1444	the transition	1431:1444	the transition from single strings to small clusters for collagen type VI (p = 0.016) and from double strings to small clusters for perlecan (p = 0.008)	1431:1582	The earliest point with a significant reduction in protein content as shown by ELISA was the transition from single strings to small clusters for collagen type VI (p = 0.016) and from double strings to small clusters for perlecan (p = 0.008), with the lowest amounts for both proteins seen in big clusters.
30711608	0	45	theme	osteoarthritic	129:142	arg1	cartilage					144:152	osteoarthritic cartilage	129:152	osteoarthritic cartilage	129:152	Changes in stiffness and biochemical composition of the pericellular matrix as a function of spatial chondrocyte organisation in osteoarthritic cartilage.
30711608	6	46	link	enzyme-linked	945:957	arg1	ELISA					980:984	ELISA	980:984	ELISA	980:984	The distribution of two major PCM components (collagen type VI and perlecan) was analysed by immunohistochemistry (8 patients) and protein content quantified by enzyme-linked immunosorbent assay (ELISA) (58 patients).
30711608	6	46	link	enzyme-linked	945:957	arg1	assay					973:977	enzyme-linked immunosorbent assay	945:977	enzyme-linked immunosorbent assay (ELISA) (58 patients)	945:999	The distribution of two major PCM components (collagen type VI and perlecan) was analysed by immunohistochemistry (8 patients) and protein content quantified by enzyme-linked immunosorbent assay (ELISA) (58 patients).
30711608	6	46	link	enzyme-linked	945:957	arg1	patients					991:998	58 patients	988:998	58 patients	988:998	The distribution of two major PCM components (collagen type VI and perlecan) was analysed by immunohistochemistry (8 patients) and protein content quantified by enzyme-linked immunosorbent assay (ELISA) (58 patients).
30711608	4	47	theme	spatial	654:660	arg1	pattern					671:677	their predominant spatial cellular pattern	636:677	their predominant spatial cellular pattern	636:677	METHOD Cartilage samples were selected according to their predominant spatial cellular pattern.
30711608	3	48	theme	functional	515:524	arg1	relevance					526:534	the functional relevance	511:534	the functional relevance of spatial organisation in articular cartilage	511:581	The aim of this study was to establish the functional relevance of spatial organisation in articular cartilage.
30711608	8	49	dep	type	1265:1268	arg1	VI					1270:1271	VI	1270:1271	VI	1270:1271	At the same time, the initially compact collagen type VI and perlecan staining progressively weakened and was less focalised.
30711608	6	50	theme	protein	915:921	arg1	content					923:929	protein content	915:929	protein content quantified by enzyme-linked immunosorbent assay (ELISA) (58 patients)	915:999	The distribution of two major PCM components (collagen type VI and perlecan) was analysed by immunohistochemistry (8 patients) and protein content quantified by enzyme-linked immunosorbent assay (ELISA) (58 patients).
30711608	7	51	theme	RESULTS	1002:1008	arg1	stiffness					1014:1022	RESULTS PCM stiffness	1002:1022	RESULTS PCM stiffness	1002:1022	RESULTS PCM stiffness significantly decreased with the development from single to double strings (p = 0.030), from double strings to small clusters (p = 0.015), and from small clusters to big clusters (p < 0.001).
30711608	10	52	theme	functional	1689:1698	arg1	biomarker					1764:1772	an image-based biomarker	1749:1772	an image-based biomarker	1749:1772	CONCLUSIONS This study demonstrates the functional relevance of spatial chondrocyte organisation as an image-based biomarker.
30711608	10	52	theme	functional	1689:1698	arg1	relevance					1700:1708	the functional relevance	1685:1708	the functional relevance of spatial chondrocyte organisation	1685:1744	CONCLUSIONS This study demonstrates the functional relevance of spatial chondrocyte organisation as an image-based biomarker.
30711608	3	53	theme	organisation	547:558	arg1	relevance					526:534	the functional relevance	511:534	the functional relevance of spatial organisation in articular cartilage	511:581	The aim of this study was to establish the functional relevance of spatial organisation in articular cartilage.
30711608	9	54	from	strings	1533:1539	arg1	point					1355:1359	The earliest point	1342:1359	The earliest point with a significant reduction in protein content as shown by ELISA	1342:1425	The earliest point with a significant reduction in protein content as shown by ELISA was the transition from single strings to small clusters for collagen type VI (p = 0.016) and from double strings to small clusters for perlecan (p = 0.008), with the lowest amounts for both proteins seen in big clusters.
30711608	9	54	from	strings	1533:1539	arg1	transition					1435:1444	the transition	1431:1444	the transition from single strings to small clusters for collagen type VI (p = 0.016) and from double strings to small clusters for perlecan (p = 0.008)	1431:1582	The earliest point with a significant reduction in protein content as shown by ELISA was the transition from single strings to small clusters for collagen type VI (p = 0.016) and from double strings to small clusters for perlecan (p = 0.008), with the lowest amounts for both proteins seen in big clusters.
30711608	5	55	theme	Young	680:684	arg1	modulus					688:694	Young's modulus	680:694	Young's modulus of their PCM	680:707	Young's modulus of their PCM was measured by atomic force microscopy (AFM) (∼500 measurements/pattern).
30711608	10	56	theme	chondrocyte	1721:1731	arg1	organisation					1733:1744	spatial chondrocyte organisation	1713:1744	spatial chondrocyte organisation	1713:1744	CONCLUSIONS This study demonstrates the functional relevance of spatial chondrocyte organisation as an image-based biomarker.
30711608	10	57	dep	CONCLUSIONS	1649:1659	arg1	demonstrates					1672:1683	demonstrates	1672:1683	demonstrates the functional relevance of spatial chondrocyte organisation as an image-based biomarker	1672:1772	CONCLUSIONS This study demonstrates the functional relevance of spatial chondrocyte organisation as an image-based biomarker.
30711608	5	58	theme	force	732:736	arg1	microscopy					738:747	atomic force microscopy	725:747	atomic force microscopy (AFM) (∼500 measurements/pattern)	725:781	Young's modulus of their PCM was measured by atomic force microscopy (AFM) (∼500 measurements/pattern).
30711608	5	58	theme	force	732:736	arg1	measurements/pattern					761:780	∼500 measurements/pattern	756:780	∼500 measurements/pattern	756:780	Young's modulus of their PCM was measured by atomic force microscopy (AFM) (∼500 measurements/pattern).
30711608	5	58	theme	force	732:736	arg1	AFM					750:752	AFM	750:752	AFM	750:752	Young's modulus of their PCM was measured by atomic force microscopy (AFM) (∼500 measurements/pattern).
30711608	11	59	theme	protein	1860:1866	arg1	degradation					1868:1878	protein degradation	1860:1878	protein degradation from double strings to small clusters	1860:1916	At the transition from single to double strings PCM stiffness decreases, followed by protein degradation from double strings to small clusters.
30711608	9	60	theme	significant	1368:1378	arg1	reduction					1380:1388	a significant reduction	1366:1388	a significant reduction in protein content as shown by ELISA	1366:1425	The earliest point with a significant reduction in protein content as shown by ELISA was the transition from single strings to small clusters for collagen type VI (p = 0.016) and from double strings to small clusters for perlecan (p = 0.008), with the lowest amounts for both proteins seen in big clusters.
30711608	7	61	from	strings	1091:1097	arg1	development					1057:1067	the development	1053:1067	the development from single to double strings (p = 0.030), from double strings to small clusters (p = 0.015), and from small clusters to big clusters (p < 0.001)	1053:1213	RESULTS PCM stiffness significantly decreased with the development from single to double strings (p = 0.030), from double strings to small clusters (p = 0.015), and from small clusters to big clusters (p < 0.001).
30711608	11	62	from	single	1798:1803	arg1	transition					1782:1791	the transition	1778:1791	the transition from single to double strings	1778:1821	At the transition from single to double strings PCM stiffness decreases, followed by protein degradation from double strings to small clusters.
30711608	8	63	theme	same	1223:1226	arg1	time					1228:1231	the same time	1219:1231	the same time	1219:1231	At the same time, the initially compact collagen type VI and perlecan staining progressively weakened and was less focalised.
30711608	9	64	theme	big	1635:1637	arg1	clusters					1639:1646	big clusters	1635:1646	big clusters	1635:1646	The earliest point with a significant reduction in protein content as shown by ELISA was the transition from single strings to small clusters for collagen type VI (p = 0.016) and from double strings to small clusters for perlecan (p = 0.008), with the lowest amounts for both proteins seen in big clusters.
30711608	9	65	with	transition	1435:1444	arg1	amounts					1601:1607	the lowest amounts	1590:1607	the lowest amounts for both proteins seen in big clusters	1590:1646	The earliest point with a significant reduction in protein content as shown by ELISA was the transition from single strings to small clusters for collagen type VI (p = 0.016) and from double strings to small clusters for perlecan (p = 0.008), with the lowest amounts for both proteins seen in big clusters.
30711608	6	66	theme	enzyme-linked	945:957	arg1	ELISA					980:984	ELISA	980:984	ELISA	980:984	The distribution of two major PCM components (collagen type VI and perlecan) was analysed by immunohistochemistry (8 patients) and protein content quantified by enzyme-linked immunosorbent assay (ELISA) (58 patients).
30711608	6	66	theme	enzyme-linked	945:957	arg1	assay					973:977	enzyme-linked immunosorbent assay	945:977	enzyme-linked immunosorbent assay (ELISA) (58 patients)	945:999	The distribution of two major PCM components (collagen type VI and perlecan) was analysed by immunohistochemistry (8 patients) and protein content quantified by enzyme-linked immunosorbent assay (ELISA) (58 patients).
30711608	6	66	theme	enzyme-linked	945:957	arg1	patients					991:998	58 patients	988:998	58 patients	988:998	The distribution of two major PCM components (collagen type VI and perlecan) was analysed by immunohistochemistry (8 patients) and protein content quantified by enzyme-linked immunosorbent assay (ELISA) (58 patients).
30711608	0	67	theme	chondrocyte	101:111	arg1	organisation					113:124	spatial chondrocyte organisation	93:124	spatial chondrocyte organisation in osteoarthritic cartilage	93:152	Changes in stiffness and biochemical composition of the pericellular matrix as a function of spatial chondrocyte organisation in osteoarthritic cartilage.
30711608	8	68	theme	collagen	1256:1263	arg1	focalised					1331:1339	focalised	1331:1339	focalised	1331:1339	At the same time, the initially compact collagen type VI and perlecan staining progressively weakened and was less focalised.
30711608	8	68	theme	collagen	1256:1263	arg1	type					1265:1268	the initially compact collagen type	1234:1268	the initially compact collagen type	1234:1268	At the same time, the initially compact collagen type VI and perlecan staining progressively weakened and was less focalised.
30711608	7	69	theme	double	1117:1122	arg1	strings					1124:1130	double strings	1117:1130	double strings to small clusters (p = 0.015)	1117:1160	RESULTS PCM stiffness significantly decreased with the development from single to double strings (p = 0.030), from double strings to small clusters (p = 0.015), and from small clusters to big clusters (p < 0.001).
30711608	9	70	theme	small	1469:1473	arg1	clusters					1475:1482	small clusters	1469:1482	small clusters for collagen type VI (p = 0.016)	1469:1515	The earliest point with a significant reduction in protein content as shown by ELISA was the transition from single strings to small clusters for collagen type VI (p = 0.016) and from double strings to small clusters for perlecan (p = 0.008), with the lowest amounts for both proteins seen in big clusters.
30711608	1	71	theme	small	278:282	arg1	clusters					292:299	single to double strings, small and big clusters	252:299	single to double strings, small and big clusters	252:299	OBJECTIVE During osteoarthritis (OA), chondrocytes seem to change their spatial arrangement from single to double strings, small and big clusters.
30711608	3	72	theme	articular	563:571	arg1	cartilage					573:581	articular cartilage	563:581	articular cartilage	563:581	The aim of this study was to establish the functional relevance of spatial organisation in articular cartilage.
30711608	10	73	theme	image-based	1752:1762	arg1	relevance					1700:1708	the functional relevance	1685:1708	the functional relevance of spatial chondrocyte organisation	1685:1744	CONCLUSIONS This study demonstrates the functional relevance of spatial chondrocyte organisation as an image-based biomarker.
30711608	10	73	theme	image-based	1752:1762	arg1	biomarker					1764:1772	an image-based biomarker	1749:1772	an image-based biomarker	1749:1772	CONCLUSIONS This study demonstrates the functional relevance of spatial chondrocyte organisation as an image-based biomarker.
30711608	8	74	theme	perlecan	1277:1284	arg1	staining					1286:1293	perlecan staining	1277:1293	perlecan staining	1277:1293	At the same time, the initially compact collagen type VI and perlecan staining progressively weakened and was less focalised.
30711608	1	75	theme	big	288:290	arg1	clusters					292:299	single to double strings, small and big clusters	252:299	single to double strings, small and big clusters	252:299	OBJECTIVE During osteoarthritis (OA), chondrocytes seem to change their spatial arrangement from single to double strings, small and big clusters.
30711608	11	76	theme	double	1885:1890	arg1	strings					1892:1898	double strings	1885:1898	double strings to small clusters	1885:1916	At the transition from single to double strings PCM stiffness decreases, followed by protein degradation from double strings to small clusters.
30711608	6	77	theme	collagen	830:837	arg1	components					818:827	two major PCM components	804:827	two major PCM components (collagen type VI and perlecan)	804:859	The distribution of two major PCM components (collagen type VI and perlecan) was analysed by immunohistochemistry (8 patients) and protein content quantified by enzyme-linked immunosorbent assay (ELISA) (58 patients).
30711608	6	77	theme	collagen	830:837	arg1	type					839:842	collagen type VI	830:845	collagen type VI	830:845	The distribution of two major PCM components (collagen type VI and perlecan) was analysed by immunohistochemistry (8 patients) and protein content quantified by enzyme-linked immunosorbent assay (ELISA) (58 patients).
30711608	2	78	theme	image-based	442:452	arg1	patterns					423:430	spatial patterns	415:430	spatial patterns	415:430	Since the pericellular matrix (PCM) appears to degrade alongside this reorganisation, it has been suggested that spatial patterns act as an image-based biomarker for OA.
30711608	2	78	theme	image-based	442:452	arg1	biomarker					454:462	an image-based biomarker	439:462	an image-based biomarker for OA	439:469	Since the pericellular matrix (PCM) appears to degrade alongside this reorganisation, it has been suggested that spatial patterns act as an image-based biomarker for OA.
30711608	0	79	from	cartilage	144:152	arg1	function					81:88	a function	79:88	a function of spatial chondrocyte organisation in osteoarthritic cartilage	79:152	Changes in stiffness and biochemical composition of the pericellular matrix as a function of spatial chondrocyte organisation in osteoarthritic cartilage.
30711608	0	80	theme	spatial	93:99	arg1	organisation					113:124	spatial chondrocyte organisation	93:124	spatial chondrocyte organisation in osteoarthritic cartilage	93:152	Changes in stiffness and biochemical composition of the pericellular matrix as a function of spatial chondrocyte organisation in osteoarthritic cartilage.
30711608	6	81	theme	components	818:827	arg1	distribution					788:799	The distribution	784:799	The distribution of two major PCM components (collagen type VI and perlecan)	784:859	The distribution of two major PCM components (collagen type VI and perlecan) was analysed by immunohistochemistry (8 patients) and protein content quantified by enzyme-linked immunosorbent assay (ELISA) (58 patients).
30711608	7	82	theme	PCM	1010:1012	arg1	stiffness					1014:1022	RESULTS PCM stiffness	1002:1022	RESULTS PCM stiffness	1002:1022	RESULTS PCM stiffness significantly decreased with the development from single to double strings (p = 0.030), from double strings to small clusters (p = 0.015), and from small clusters to big clusters (p < 0.001).
30711608	6	83	dep	type	839:842	arg1	VI					844:845	VI	844:845	VI	844:845	The distribution of two major PCM components (collagen type VI and perlecan) was analysed by immunohistochemistry (8 patients) and protein content quantified by enzyme-linked immunosorbent assay (ELISA) (58 patients).
30711608	6	84	theme	major	808:812	arg1	perlecan					851:858	perlecan	851:858	perlecan	851:858	The distribution of two major PCM components (collagen type VI and perlecan) was analysed by immunohistochemistry (8 patients) and protein content quantified by enzyme-linked immunosorbent assay (ELISA) (58 patients).
30711608	6	84	theme	major	808:812	arg1	components					818:827	two major PCM components	804:827	two major PCM components (collagen type VI and perlecan)	804:859	The distribution of two major PCM components (collagen type VI and perlecan) was analysed by immunohistochemistry (8 patients) and protein content quantified by enzyme-linked immunosorbent assay (ELISA) (58 patients).
30711608	6	84	theme	major	808:812	arg1	type					839:842	collagen type VI	830:845	collagen type VI	830:845	The distribution of two major PCM components (collagen type VI and perlecan) was analysed by immunohistochemistry (8 patients) and protein content quantified by enzyme-linked immunosorbent assay (ELISA) (58 patients).
30711608	9	85	from	reduction	1380:1388	arg1	content					1401:1407	protein content	1393:1407	protein content as shown by ELISA	1393:1425	The earliest point with a significant reduction in protein content as shown by ELISA was the transition from single strings to small clusters for collagen type VI (p = 0.016) and from double strings to small clusters for perlecan (p = 0.008), with the lowest amounts for both proteins seen in big clusters.
30711608	7	86	theme	big	1190:1192	arg1	clusters					1194:1201	big clusters	1190:1201	big clusters (p < 0.001)	1190:1213	RESULTS PCM stiffness significantly decreased with the development from single to double strings (p = 0.030), from double strings to small clusters (p = 0.015), and from small clusters to big clusters (p < 0.001).
30711608	7	86	theme	big	1190:1192	arg1	p < 0.001					1204:1212	p < 0.001	1204:1212	p < 0.001	1204:1212	RESULTS PCM stiffness significantly decreased with the development from single to double strings (p = 0.030), from double strings to small clusters (p = 0.015), and from small clusters to big clusters (p < 0.001).
30711608	4	87	theme	METHOD	584:589	arg1	samples					601:607	METHOD Cartilage samples	584:607	METHOD Cartilage samples	584:607	METHOD Cartilage samples were selected according to their predominant spatial cellular pattern.
30711608	9	88	with	point	1355:1359	arg1	reduction					1380:1388	a significant reduction	1366:1388	a significant reduction in protein content as shown by ELISA	1366:1425	The earliest point with a significant reduction in protein content as shown by ELISA was the transition from single strings to small clusters for collagen type VI (p = 0.016) and from double strings to small clusters for perlecan (p = 0.008), with the lowest amounts for both proteins seen in big clusters.
30711608	2	89	theme	pericellular	312:323	arg1	PCM					333:335	PCM	333:335	PCM	333:335	Since the pericellular matrix (PCM) appears to degrade alongside this reorganisation, it has been suggested that spatial patterns act as an image-based biomarker for OA.
30711608	2	89	theme	pericellular	312:323	arg1	matrix					325:330	the pericellular matrix	308:330	the pericellular matrix (PCM)	308:336	Since the pericellular matrix (PCM) appears to degrade alongside this reorganisation, it has been suggested that spatial patterns act as an image-based biomarker for OA.
30711608	1	90	theme	spatial	227:233	arg1	arrangement					235:245	their spatial arrangement	221:245	their spatial arrangement from single to double strings, small and big clusters	221:299	OBJECTIVE During osteoarthritis (OA), chondrocytes seem to change their spatial arrangement from single to double strings, small and big clusters.
30711608	0	91	from	organisation	113:124	arg1	cartilage					144:152	osteoarthritic cartilage	129:152	osteoarthritic cartilage	129:152	Changes in stiffness and biochemical composition of the pericellular matrix as a function of spatial chondrocyte organisation in osteoarthritic cartilage.
30711608	3	92	theme	study	488:492	arg1	aim					476:478	The aim	472:478	The aim of this study	472:492	The aim of this study was to establish the functional relevance of spatial organisation in articular cartilage.
30982137	10	0	gly	defucosylated	1279:1291	arg1	antibody					1293:1300	the defucosylated antibody	1275:1300	the defucosylated antibody	1275:1300	The antibody-dependent cellular cytotoxicity activity of the defucosylated antibody increased 14-fold compared to the wild-type antibody, which was the same as our previous results.
30982137	4	1	theme	established	609:619	arg1	line					678:681	an established α-1,6-fucosyltransferase (FUT8) gene knockout CHO-S cell line	606:681	an established α-1,6-fucosyltransferase (FUT8) gene knockout CHO-S cell line	606:681	We engineered a stable cell line expressing defucosylated anti-HER2 antibody based on an established α-1,6-fucosyltransferase (FUT8) gene knockout CHO-S cell line.
30982137	8	2	theme	antibody	990:997	arg1	production					966:975	The production	962:975	The production of anti-HER2 antibody from the FUT8 gene knockout CHO-S cells in the bioreactor	962:1055	The production of anti-HER2 antibody from the FUT8 gene knockout CHO-S cells in the bioreactor increased by 37% compared to the shake flask system.
30982137	5	3	theme	medium	704:709	arg1	optimization					688:699	The optimization	684:699	The optimization of medium and feed	684:718	The optimization of medium and feed was evaluated in a small-scale culture system.
30982137	6	4	theme	bioreactor	820:829	arg1	system					831:836	a bioreactor system	818:836	a bioreactor system	818:836	Then the optimal medium and feed were scaled up in a bioreactor system.
30982137	11	5	theme	bioprocess	1419:1428	arg1	development					1430:1440	our bioprocess development	1415:1440	our bioprocess development	1415:1440	The results of our bioprocess development demonstrated that the engineered cell line could be developed to a biopharmaceutical industrial cell line.
30982137	9	6	theme	flask	1204:1208	arg1	system					1210:1215	shake flask system	1198:1215	shake flask system	1198:1215	The N-glycan profile of the produced antibody was consistent between the bioreactor and shake flask system.
30982137	8	7	theme	gene	1013:1016	arg1	cells					1033:1037	the FUT8 gene knockout CHO-S cells	1004:1037	the FUT8 gene knockout CHO-S cells	1004:1037	The production of anti-HER2 antibody from the FUT8 gene knockout CHO-S cells in the bioreactor increased by 37% compared to the shake flask system.
30982137	2	8	theme	clonal	319:324	arg1	lines					345:349	stable and clonal manufacturing cell lines	308:349	stable and clonal manufacturing cell lines	308:349	The establishment of stable and clonal manufacturing cell lines is the basis of therapeutic antibodies production.
30982137	8	9	theme	CHO-S	1027:1031	arg1	cells					1033:1037	the FUT8 gene knockout CHO-S cells	1004:1037	the FUT8 gene knockout CHO-S cells	1004:1037	The production of anti-HER2 antibody from the FUT8 gene knockout CHO-S cells in the bioreactor increased by 37% compared to the shake flask system.
30982137	4	10	theme	cell	673:676	arg1	line					678:681	an established α-1,6-fucosyltransferase (FUT8) gene knockout CHO-S cell line	606:681	an established α-1,6-fucosyltransferase (FUT8) gene knockout CHO-S cell line	606:681	We engineered a stable cell line expressing defucosylated anti-HER2 antibody based on an established α-1,6-fucosyltransferase (FUT8) gene knockout CHO-S cell line.
30982137	2	11	theme	stable	308:313	arg1	lines					345:349	stable and clonal manufacturing cell lines	308:349	stable and clonal manufacturing cell lines	308:349	The establishment of stable and clonal manufacturing cell lines is the basis of therapeutic antibodies production.
30982137	1	12	theme	clinical	201:208	arg1	practices					210:218	clinical practices	201:218	clinical practices	201:218	In recent years, an increasing number of defucosylated therapeutic antibodies have been applied in clinical practices due to their better efficacy compared to fucosylated counterparts.
30982137	5	13	theme	culture	751:757	arg1	system					759:764	a small-scale culture system	737:764	a small-scale culture system	737:764	The optimization of medium and feed was evaluated in a small-scale culture system.
30982137	5	14	theme	feed	715:718	arg1	optimization					688:699	The optimization	684:699	The optimization of medium and feed	684:718	The optimization of medium and feed was evaluated in a small-scale culture system.
30982137	10	15	theme	cytotoxicity	1250:1261	arg1	activity					1263:1270	The antibody-dependent cellular cytotoxicity activity	1218:1270	The antibody-dependent cellular cytotoxicity activity of the defucosylated antibody	1218:1300	The antibody-dependent cellular cytotoxicity activity of the defucosylated antibody increased 14-fold compared to the wild-type antibody, which was the same as our previous results.
30982137	1	16	gly	defucosylated	143:155	arg1	antibodies					169:178	defucosylated therapeutic antibodies	143:178	defucosylated therapeutic antibodies	143:178	In recent years, an increasing number of defucosylated therapeutic antibodies have been applied in clinical practices due to their better efficacy compared to fucosylated counterparts.
30982137	1	17	theme	increasing	122:131	arg1	number					133:138	an increasing number	119:138	an increasing number of defucosylated therapeutic antibodies	119:178	In recent years, an increasing number of defucosylated therapeutic antibodies have been applied in clinical practices due to their better efficacy compared to fucosylated counterparts.
30982137	10	18	theme	antibody-dependent	1222:1239	arg1	cytotoxicity					1250:1261	antibody-dependent cellular cytotoxicity	1222:1261	The antibody-dependent cellular cytotoxicity activity of the defucosylated antibody	1218:1300	The antibody-dependent cellular cytotoxicity activity of the defucosylated antibody increased 14-fold compared to the wild-type antibody, which was the same as our previous results.
30982137	10	19	theme	defucosylated	1279:1291	arg1	antibody					1293:1300	the defucosylated antibody	1275:1300	the defucosylated antibody	1275:1300	The antibody-dependent cellular cytotoxicity activity of the defucosylated antibody increased 14-fold compared to the wild-type antibody, which was the same as our previous results.
30982137	0	20	theme	defucosylated	68:80	arg1	antibody					92:99	defucosylated anti-HER2 antibody	68:99	defucosylated anti-HER2 antibody	68:99	Bioprocess development of a stable FUT8-/--CHO cell line to produce defucosylated anti-HER2 antibody.
30982137	1	21	theme	therapeutic	157:167	arg1	antibodies					169:178	defucosylated therapeutic antibodies	143:178	defucosylated therapeutic antibodies	143:178	In recent years, an increasing number of defucosylated therapeutic antibodies have been applied in clinical practices due to their better efficacy compared to fucosylated counterparts.
30982137	11	22	theme	cell	1475:1478	arg1	line					1480:1483	the engineered cell line	1460:1483	the engineered cell line	1460:1483	The results of our bioprocess development demonstrated that the engineered cell line could be developed to a biopharmaceutical industrial cell line.
30982137	3	23	theme	Bioprocess	402:411	arg1	development					413:423	Bioprocess development	402:423	Bioprocess development of a new cell line	402:442	Bioprocess development of a new cell line is necessary for its future applications in the biopharmaceutical industry.
30982137	3	24	theme	line	439:442	arg1	development					413:423	Bioprocess development	402:423	Bioprocess development of a new cell line	402:442	Bioprocess development of a new cell line is necessary for its future applications in the biopharmaceutical industry.
30982137	0	25	theme	Bioprocess	0:9	arg1	development					11:21	Bioprocess development	0:21	Bioprocess development of a stable FUT8-/--CHO cell line	0:55	Bioprocess development of a stable FUT8-/--CHO cell line to produce defucosylated anti-HER2 antibody.
30982137	4	26	theme	anti-HER2	578:586	arg1	antibody					588:595	defucosylated anti-HER2 antibody	564:595	defucosylated anti-HER2 antibody	564:595	We engineered a stable cell line expressing defucosylated anti-HER2 antibody based on an established α-1,6-fucosyltransferase (FUT8) gene knockout CHO-S cell line.
30982137	1	27	theme	recent	105:110	arg1	years					112:116	recent years	105:116	recent years	105:116	In recent years, an increasing number of defucosylated therapeutic antibodies have been applied in clinical practices due to their better efficacy compared to fucosylated counterparts.
30982137	3	28	theme	new	430:432	arg1	line					439:442	a new cell line	428:442	a new cell line	428:442	Bioprocess development of a new cell line is necessary for its future applications in the biopharmaceutical industry.
30982137	9	29	theme	N-glycan	1114:1121	arg1	profile					1123:1129	The N-glycan profile	1110:1129	The N-glycan profile of the produced antibody	1110:1154	The N-glycan profile of the produced antibody was consistent between the bioreactor and shake flask system.
30982137	9	29	theme	N-glycan	1114:1121	arg1	consistent					1160:1169	consistent	1160:1169	consistent	1160:1169	The N-glycan profile of the produced antibody was consistent between the bioreactor and shake flask system.
30982137	0	30	theme	stable	28:33	arg1	line					52:55	a stable FUT8-/--CHO cell line	26:55	a stable FUT8-/--CHO cell line	26:55	Bioprocess development of a stable FUT8-/--CHO cell line to produce defucosylated anti-HER2 antibody.
30982137	8	31	theme	shake	1090:1094	arg1	system					1102:1107	the shake flask system	1086:1107	the shake flask system	1086:1107	The production of anti-HER2 antibody from the FUT8 gene knockout CHO-S cells in the bioreactor increased by 37% compared to the shake flask system.
30982137	4	32	theme	cell	543:546	arg1	line					548:551	a stable cell line	534:551	a stable cell line expressing defucosylated anti-HER2 antibody based on an established α-1,6-fucosyltransferase (FUT8) gene knockout CHO-S cell line	534:681	We engineered a stable cell line expressing defucosylated anti-HER2 antibody based on an established α-1,6-fucosyltransferase (FUT8) gene knockout CHO-S cell line.
30982137	0	33	theme	cell	47:50	arg1	line					52:55	a stable FUT8-/--CHO cell line	26:55	a stable FUT8-/--CHO cell line	26:55	Bioprocess development of a stable FUT8-/--CHO cell line to produce defucosylated anti-HER2 antibody.
30982137	9	34	theme	produced	1138:1145	arg1	antibody					1147:1154	the produced antibody	1134:1154	the produced antibody	1134:1154	The N-glycan profile of the produced antibody was consistent between the bioreactor and shake flask system.
30982137	2	35	theme	lines	345:349	arg1	establishment					291:303	The establishment	287:303	The establishment of stable and clonal manufacturing cell lines	287:349	The establishment of stable and clonal manufacturing cell lines is the basis of therapeutic antibodies production.
30982137	2	35	theme	lines	345:349	arg1	basis					358:362	the basis	354:362	the basis of therapeutic antibodies production	354:399	The establishment of stable and clonal manufacturing cell lines is the basis of therapeutic antibodies production.
30982137	7	36	theme	glycan	923:928	arg1	compositions					930:941	glycan compositions	923:941	glycan compositions	923:941	After fed-batch culture over 13 days, we evaluated the cell growth, antibody yield, glycan compositions and bioactivities.
30982137	4	37	theme	FUT8	647:650	arg1	line					678:681	an established α-1,6-fucosyltransferase (FUT8) gene knockout CHO-S cell line	606:681	an established α-1,6-fucosyltransferase (FUT8) gene knockout CHO-S cell line	606:681	We engineered a stable cell line expressing defucosylated anti-HER2 antibody based on an established α-1,6-fucosyltransferase (FUT8) gene knockout CHO-S cell line.
30982137	10	38	theme	previous	1382:1389	arg1	results					1391:1397	our previous results	1378:1397	our previous results	1378:1397	The antibody-dependent cellular cytotoxicity activity of the defucosylated antibody increased 14-fold compared to the wild-type antibody, which was the same as our previous results.
30982137	2	39	theme	antibodies	379:388	arg1	production					390:399	therapeutic antibodies production	367:399	therapeutic antibodies production	367:399	The establishment of stable and clonal manufacturing cell lines is the basis of therapeutic antibodies production.
30982137	1	40	theme	better	233:238	arg1	efficacy					240:247	their better efficacy	227:247	their better efficacy compared to fucosylated counterparts	227:284	In recent years, an increasing number of defucosylated therapeutic antibodies have been applied in clinical practices due to their better efficacy compared to fucosylated counterparts.
30982137	8	41	theme	anti-HER2	980:988	arg1	antibody					990:997	anti-HER2 antibody	980:997	anti-HER2 antibody	980:997	The production of anti-HER2 antibody from the FUT8 gene knockout CHO-S cells in the bioreactor increased by 37% compared to the shake flask system.
30982137	4	42	theme	gene	653:656	arg1	line					678:681	an established α-1,6-fucosyltransferase (FUT8) gene knockout CHO-S cell line	606:681	an established α-1,6-fucosyltransferase (FUT8) gene knockout CHO-S cell line	606:681	We engineered a stable cell line expressing defucosylated anti-HER2 antibody based on an established α-1,6-fucosyltransferase (FUT8) gene knockout CHO-S cell line.
30982137	11	43	theme	development	1430:1440	arg1	results					1404:1410	The results	1400:1410	The results of our bioprocess development	1400:1440	The results of our bioprocess development demonstrated that the engineered cell line could be developed to a biopharmaceutical industrial cell line.
30982137	7	44	theme	cell	894:897	arg1	growth					899:904	cell growth	894:904	cell growth	894:904	After fed-batch culture over 13 days, we evaluated the cell growth, antibody yield, glycan compositions and bioactivities.
30982137	1	45	theme	fucosylated	261:271	arg1	counterparts					273:284	fucosylated counterparts	261:284	fucosylated counterparts	261:284	In recent years, an increasing number of defucosylated therapeutic antibodies have been applied in clinical practices due to their better efficacy compared to fucosylated counterparts.
30982137	8	46	theme	FUT8	1008:1011	arg1	cells					1033:1037	the FUT8 gene knockout CHO-S cells	1004:1037	the FUT8 gene knockout CHO-S cells	1004:1037	The production of anti-HER2 antibody from the FUT8 gene knockout CHO-S cells in the bioreactor increased by 37% compared to the shake flask system.
30982137	4	47	theme	α-1,6-fucosyltransferase	621:644	arg1	line					678:681	an established α-1,6-fucosyltransferase (FUT8) gene knockout CHO-S cell line	606:681	an established α-1,6-fucosyltransferase (FUT8) gene knockout CHO-S cell line	606:681	We engineered a stable cell line expressing defucosylated anti-HER2 antibody based on an established α-1,6-fucosyltransferase (FUT8) gene knockout CHO-S cell line.
30982137	9	48	theme	shake	1198:1202	arg1	system					1210:1215	shake flask system	1198:1215	shake flask system	1198:1215	The N-glycan profile of the produced antibody was consistent between the bioreactor and shake flask system.
30982137	1	49	theme	antibodies	169:178	arg1	number					133:138	an increasing number	119:138	an increasing number of defucosylated therapeutic antibodies	119:178	In recent years, an increasing number of defucosylated therapeutic antibodies have been applied in clinical practices due to their better efficacy compared to fucosylated counterparts.
30982137	8	50	theme	knockout	1018:1025	arg1	cells					1033:1037	the FUT8 gene knockout CHO-S cells	1004:1037	the FUT8 gene knockout CHO-S cells	1004:1037	The production of anti-HER2 antibody from the FUT8 gene knockout CHO-S cells in the bioreactor increased by 37% compared to the shake flask system.
30982137	2	51	theme	manufacturing	326:338	arg1	lines					345:349	stable and clonal manufacturing cell lines	308:349	stable and clonal manufacturing cell lines	308:349	The establishment of stable and clonal manufacturing cell lines is the basis of therapeutic antibodies production.
30982137	4	52	theme	CHO-S	667:671	arg1	line					678:681	an established α-1,6-fucosyltransferase (FUT8) gene knockout CHO-S cell line	606:681	an established α-1,6-fucosyltransferase (FUT8) gene knockout CHO-S cell line	606:681	We engineered a stable cell line expressing defucosylated anti-HER2 antibody based on an established α-1,6-fucosyltransferase (FUT8) gene knockout CHO-S cell line.
30982137	5	53	theme	small-scale	739:749	arg1	system					759:764	a small-scale culture system	737:764	a small-scale culture system	737:764	The optimization of medium and feed was evaluated in a small-scale culture system.
30982137	7	54	theme	fed-batch	845:853	arg1	culture					855:861	fed-batch culture	845:861	fed-batch culture over 13 days	845:874	After fed-batch culture over 13 days, we evaluated the cell growth, antibody yield, glycan compositions and bioactivities.
30982137	11	55	theme	biopharmaceutical	1509:1525	arg1	line					1543:1546	a biopharmaceutical industrial cell line	1507:1546	a biopharmaceutical industrial cell line	1507:1546	The results of our bioprocess development demonstrated that the engineered cell line could be developed to a biopharmaceutical industrial cell line.
30982137	6	56	theme	optimal	776:782	arg1	medium					784:789	Then the optimal medium	767:789	Then the optimal medium	767:789	Then the optimal medium and feed were scaled up in a bioreactor system.
30982137	10	57	theme	cellular	1241:1248	arg1	cytotoxicity					1250:1261	antibody-dependent cellular cytotoxicity	1222:1261	The antibody-dependent cellular cytotoxicity activity of the defucosylated antibody	1218:1300	The antibody-dependent cellular cytotoxicity activity of the defucosylated antibody increased 14-fold compared to the wild-type antibody, which was the same as our previous results.
30982137	10	58	dep	increased	1302:1310	arg1	compared					1320:1327	compared	1320:1327	increased 14-fold compared to the wild-type antibody, which was the same as our previous results	1302:1397	The antibody-dependent cellular cytotoxicity activity of the defucosylated antibody increased 14-fold compared to the wild-type antibody, which was the same as our previous results.
30982137	10	59	theme	antibody	1293:1300	arg1	activity					1263:1270	The antibody-dependent cellular cytotoxicity activity	1218:1270	The antibody-dependent cellular cytotoxicity activity of the defucosylated antibody	1218:1300	The antibody-dependent cellular cytotoxicity activity of the defucosylated antibody increased 14-fold compared to the wild-type antibody, which was the same as our previous results.
30982137	0	60	theme	anti-HER2	82:90	arg1	antibody					92:99	defucosylated anti-HER2 antibody	68:99	defucosylated anti-HER2 antibody	68:99	Bioprocess development of a stable FUT8-/--CHO cell line to produce defucosylated anti-HER2 antibody.
30982137	4	61	gly	defucosylated	564:576	arg1	antibody					588:595	defucosylated anti-HER2 antibody	564:595	defucosylated anti-HER2 antibody	564:595	We engineered a stable cell line expressing defucosylated anti-HER2 antibody based on an established α-1,6-fucosyltransferase (FUT8) gene knockout CHO-S cell line.
30982137	1	62	theme	defucosylated	143:155	arg1	antibodies					169:178	defucosylated therapeutic antibodies	143:178	defucosylated therapeutic antibodies	143:178	In recent years, an increasing number of defucosylated therapeutic antibodies have been applied in clinical practices due to their better efficacy compared to fucosylated counterparts.
30982137	4	63	theme	stable	536:541	arg1	line					548:551	a stable cell line	534:551	a stable cell line expressing defucosylated anti-HER2 antibody based on an established α-1,6-fucosyltransferase (FUT8) gene knockout CHO-S cell line	534:681	We engineered a stable cell line expressing defucosylated anti-HER2 antibody based on an established α-1,6-fucosyltransferase (FUT8) gene knockout CHO-S cell line.
30982137	11	64	theme	cell	1538:1541	arg1	line					1543:1546	a biopharmaceutical industrial cell line	1507:1546	a biopharmaceutical industrial cell line	1507:1546	The results of our bioprocess development demonstrated that the engineered cell line could be developed to a biopharmaceutical industrial cell line.
30982137	11	65	theme	engineered	1464:1473	arg1	line					1480:1483	the engineered cell line	1460:1483	the engineered cell line	1460:1483	The results of our bioprocess development demonstrated that the engineered cell line could be developed to a biopharmaceutical industrial cell line.
30982137	3	66	theme	biopharmaceutical	492:508	arg1	industry					510:517	the biopharmaceutical industry	488:517	the biopharmaceutical industry	488:517	Bioprocess development of a new cell line is necessary for its future applications in the biopharmaceutical industry.
30982137	0	67	gly	defucosylated	68:80	arg1	antibody					92:99	defucosylated anti-HER2 antibody	68:99	defucosylated anti-HER2 antibody	68:99	Bioprocess development of a stable FUT8-/--CHO cell line to produce defucosylated anti-HER2 antibody.
30982137	2	68	theme	production	390:399	arg1	establishment					291:303	The establishment	287:303	The establishment of stable and clonal manufacturing cell lines	287:349	The establishment of stable and clonal manufacturing cell lines is the basis of therapeutic antibodies production.
30982137	2	68	theme	production	390:399	arg1	basis					358:362	the basis	354:362	the basis of therapeutic antibodies production	354:399	The establishment of stable and clonal manufacturing cell lines is the basis of therapeutic antibodies production.
30982137	1	69	gly	fucosylated	261:271	arg1	counterparts					273:284	fucosylated counterparts	261:284	fucosylated counterparts	261:284	In recent years, an increasing number of defucosylated therapeutic antibodies have been applied in clinical practices due to their better efficacy compared to fucosylated counterparts.
30982137	10	70	theme	wild-type	1336:1344	arg1	same					1370:1373	same	1370:1373	same	1370:1373	The antibody-dependent cellular cytotoxicity activity of the defucosylated antibody increased 14-fold compared to the wild-type antibody, which was the same as our previous results.
30982137	10	70	theme	wild-type	1336:1344	arg1	antibody					1346:1353	the wild-type antibody	1332:1353	the wild-type antibody	1332:1353	The antibody-dependent cellular cytotoxicity activity of the defucosylated antibody increased 14-fold compared to the wild-type antibody, which was the same as our previous results.
30982137	0	71	theme	FUT8-/--CHO	35:45	arg1	line					52:55	a stable FUT8-/--CHO cell line	26:55	a stable FUT8-/--CHO cell line	26:55	Bioprocess development of a stable FUT8-/--CHO cell line to produce defucosylated anti-HER2 antibody.
30982137	8	72	theme	flask	1096:1100	arg1	system					1102:1107	the shake flask system	1086:1107	the shake flask system	1086:1107	The production of anti-HER2 antibody from the FUT8 gene knockout CHO-S cells in the bioreactor increased by 37% compared to the shake flask system.
30982137	8	73	from	production	966:975	arg1	bioreactor					1046:1055	the bioreactor	1042:1055	the bioreactor	1042:1055	The production of anti-HER2 antibody from the FUT8 gene knockout CHO-S cells in the bioreactor increased by 37% compared to the shake flask system.
30982137	3	74	theme	cell	434:437	arg1	line					439:442	a new cell line	428:442	a new cell line	428:442	Bioprocess development of a new cell line is necessary for its future applications in the biopharmaceutical industry.
30982137	3	75	theme	future	465:470	arg1	applications					472:483	its future applications	461:483	its future applications in the biopharmaceutical industry	461:517	Bioprocess development of a new cell line is necessary for its future applications in the biopharmaceutical industry.
30982137	0	76	theme	line	52:55	arg1	development					11:21	Bioprocess development	0:21	Bioprocess development of a stable FUT8-/--CHO cell line	0:55	Bioprocess development of a stable FUT8-/--CHO cell line to produce defucosylated anti-HER2 antibody.
30982137	11	77	theme	industrial	1527:1536	arg1	line					1543:1546	a biopharmaceutical industrial cell line	1507:1546	a biopharmaceutical industrial cell line	1507:1546	The results of our bioprocess development demonstrated that the engineered cell line could be developed to a biopharmaceutical industrial cell line.
30982137	4	78	theme	defucosylated	564:576	arg1	antibody					588:595	defucosylated anti-HER2 antibody	564:595	defucosylated anti-HER2 antibody	564:595	We engineered a stable cell line expressing defucosylated anti-HER2 antibody based on an established α-1,6-fucosyltransferase (FUT8) gene knockout CHO-S cell line.
30982137	3	79	from	applications	472:483	arg1	industry					510:517	the biopharmaceutical industry	488:517	the biopharmaceutical industry	488:517	Bioprocess development of a new cell line is necessary for its future applications in the biopharmaceutical industry.
30982137	9	80	theme	antibody	1147:1154	arg1	profile					1123:1129	The N-glycan profile	1110:1129	The N-glycan profile of the produced antibody	1110:1154	The N-glycan profile of the produced antibody was consistent between the bioreactor and shake flask system.
30982137	9	80	theme	antibody	1147:1154	arg1	consistent					1160:1169	consistent	1160:1169	consistent	1160:1169	The N-glycan profile of the produced antibody was consistent between the bioreactor and shake flask system.
30982137	2	81	theme	cell	340:343	arg1	lines					345:349	stable and clonal manufacturing cell lines	308:349	stable and clonal manufacturing cell lines	308:349	The establishment of stable and clonal manufacturing cell lines is the basis of therapeutic antibodies production.
30982137	7	82	theme	antibody	907:914	arg1	yield					916:920	antibody yield	907:920	antibody yield	907:920	After fed-batch culture over 13 days, we evaluated the cell growth, antibody yield, glycan compositions and bioactivities.
30982137	8	83	from	cells	1033:1037	arg1	production					966:975	The production	962:975	The production of anti-HER2 antibody from the FUT8 gene knockout CHO-S cells in the bioreactor	962:1055	The production of anti-HER2 antibody from the FUT8 gene knockout CHO-S cells in the bioreactor increased by 37% compared to the shake flask system.
30982137	4	84	theme	knockout	658:665	arg1	line					678:681	an established α-1,6-fucosyltransferase (FUT8) gene knockout CHO-S cell line	606:681	an established α-1,6-fucosyltransferase (FUT8) gene knockout CHO-S cell line	606:681	We engineered a stable cell line expressing defucosylated anti-HER2 antibody based on an established α-1,6-fucosyltransferase (FUT8) gene knockout CHO-S cell line.
30982137	2	85	theme	therapeutic	367:377	arg1	antibodies					379:388	therapeutic antibodies	367:388	therapeutic antibodies production	367:399	The establishment of stable and clonal manufacturing cell lines is the basis of therapeutic antibodies production.
31136099	6	0	theme	complement	1124:1133	arg1	C3					1135:1136	human complement C3	1118:1136	human complement C3	1118:1136	Postproteomic site-specific N-glycan analyses showed that human complement C3 bears high-mannose and hybrid glycoforms rather than complex glycoforms at Asn85.
31136099	9	1	theme	hepatobiliary	1768:1780	arg1	cancers					1782:1788	hepatobiliary cancers	1768:1788	hepatobiliary cancers	1768:1788	In conclusion, our data show that particular plasma N-glycoproteins with specific N-glycan compositions could be potential noninvasive markers to evaluate oncological status and prognosis of hepatobiliary cancers.
31136099	9	2	theme	potential	1690:1698	arg1	markers					1712:1718	potential noninvasive markers	1690:1718	potential noninvasive markers	1690:1718	In conclusion, our data show that particular plasma N-glycoproteins with specific N-glycan compositions could be potential noninvasive markers to evaluate oncological status and prognosis of hepatobiliary cancers.
31136099	9	3	with	N-glycoproteins	1629:1643	arg1	compositions					1668:1679	specific N-glycan compositions	1650:1679	specific N-glycan compositions	1650:1679	In conclusion, our data show that particular plasma N-glycoproteins with specific N-glycan compositions could be potential noninvasive markers to evaluate oncological status and prognosis of hepatobiliary cancers.
31136099	2	4	theme	Appropriate	157:167	arg1	markers					169:175	Appropriate markers	157:175	Appropriate markers for early diagnosis, monitoring of disease progression, and prediction of postsurgical outcome	157:270	Appropriate markers for early diagnosis, monitoring of disease progression, and prediction of postsurgical outcome are still lacking.
31136099	8	5	from	Asn85	1435:1439	arg1	glycoform					1422:1430	a hybrid glycoform	1413:1430	a hybrid glycoform at Asn85 of complement C3	1413:1456	Furthermore, stepwise Cox regression analyses revealed that HCC patients with a hybrid glycoform at Asn85 of complement C3 had a lower postsurgery tumor recurrence rate or mortality rate than those with a low amount of complement C3 protein.
31136099	4	6	theme	proteomic	638:646	arg1	approach					648:655	a mass spectrometry-based quantitative proteomic approach	599:655	a mass spectrometry-based quantitative proteomic approach	599:655	Using a mass spectrometry-based quantitative proteomic approach, we found that 57 of 5358 identified plasma proteins were differentially expressed in hepatobiliary cancers.
31136099	8	7	theme	postsurgery	1470:1480	arg1	rate					1499:1502	a lower postsurgery tumor recurrence rate	1462:1502	a lower postsurgery tumor recurrence rate	1462:1502	Furthermore, stepwise Cox regression analyses revealed that HCC patients with a hybrid glycoform at Asn85 of complement C3 had a lower postsurgery tumor recurrence rate or mortality rate than those with a low amount of complement C3 protein.
31136099	7	8	with	abundance	1224:1232	arg1	glycoform					1279:1287	mannose-5 or mannose-6 glycoform	1256:1287	mannose-5 or mannose-6 glycoform	1256:1287	The abundance of complement C3 with mannose-5 or mannose-6 glycoform at Asn85 was associated with HCC tumor grade.
31136099	3	9	theme	N-glycoproteins	322:336	arg1	majority					298:305	the majority	294:305	the majority of circulating N-glycoproteins	294:336	As the majority of circulating N-glycoproteins are originated from the hepatobiliary system, we sought to explore new markers by assessing the dynamics of N-glycoproteome in plasma samples from patients with hepatocellular carcinoma (HCC), cholangiocarcinoma (CCA), or combined HCC and CCA (cHCC-CCA).
31136099	8	10	theme	recurrence	1488:1497	arg1	rate					1499:1502	a lower postsurgery tumor recurrence rate	1462:1502	a lower postsurgery tumor recurrence rate	1462:1502	Furthermore, stepwise Cox regression analyses revealed that HCC patients with a hybrid glycoform at Asn85 of complement C3 had a lower postsurgery tumor recurrence rate or mortality rate than those with a low amount of complement C3 protein.
31136099	5	11	from	C-III	848:852	arg1	HCC					857:859	HCC	857:859	HCC	857:859	The levels of four essential proteins, including complement C3 and apolipoprotein C-III in HCC, galectin-3-binding protein in CCA, and 72 kDa inositol polyphosphate 5-phosphatase in cHCC-CCA, were highly correlated with tumor stage, tumor grade, recurrence-free survival, and overall survival.
31136099	5	11	from	C-III	848:852	arg1	cHCC-CCA					948:955	cHCC-CCA	948:955	cHCC-CCA	948:955	The levels of four essential proteins, including complement C3 and apolipoprotein C-III in HCC, galectin-3-binding protein in CCA, and 72 kDa inositol polyphosphate 5-phosphatase in cHCC-CCA, were highly correlated with tumor stage, tumor grade, recurrence-free survival, and overall survival.
31136099	5	11	from	C-III	848:852	arg1	CCA					892:894	CCA	892:894	CCA	892:894	The levels of four essential proteins, including complement C3 and apolipoprotein C-III in HCC, galectin-3-binding protein in CCA, and 72 kDa inositol polyphosphate 5-phosphatase in cHCC-CCA, were highly correlated with tumor stage, tumor grade, recurrence-free survival, and overall survival.
31136099	1	12	theme	cancer	133:138	arg1	worldwide					146:154	cancer death worldwide	133:154	cancer death worldwide	133:154	Hepatobiliary cancer is the third leading cause of cancer death worldwide.
31136099	8	13	with	patients	1399:1406	arg1	glycoform					1422:1430	a hybrid glycoform	1413:1430	a hybrid glycoform at Asn85 of complement C3	1413:1456	Furthermore, stepwise Cox regression analyses revealed that HCC patients with a hybrid glycoform at Asn85 of complement C3 had a lower postsurgery tumor recurrence rate or mortality rate than those with a low amount of complement C3 protein.
31136099	1	14	theme	worldwide	146:154	arg1	cancer					96:101	Hepatobiliary cancer	82:101	Hepatobiliary cancer	82:101	Hepatobiliary cancer is the third leading cause of cancer death worldwide.
31136099	1	14	theme	worldwide	146:154	arg1	cause					124:128	the third leading cause	106:128	the third leading cause of cancer death worldwide	106:154	Hepatobiliary cancer is the third leading cause of cancer death worldwide.
31136099	6	15	contain	bears	1138:1142	arg1	C3					1135:1136	human complement C3	1118:1136	human complement C3	1118:1136	Postproteomic site-specific N-glycan analyses showed that human complement C3 bears high-mannose and hybrid glycoforms rather than complex glycoforms at Asn85.
31136099	6	15	contain	bears	1138:1142	arg2	glycoforms					1168:1177	high-mannose and hybrid glycoforms	1144:1177	high-mannose and hybrid glycoforms rather than complex glycoforms at Asn85	1144:1217	Postproteomic site-specific N-glycan analyses showed that human complement C3 bears high-mannose and hybrid glycoforms rather than complex glycoforms at Asn85.
31136099	6	15	contain	bears	1138:1142	arg2	glycoforms					1199:1208	complex glycoforms	1191:1208	high-mannose and hybrid glycoforms rather than complex glycoforms at Asn85	1144:1217	Postproteomic site-specific N-glycan analyses showed that human complement C3 bears high-mannose and hybrid glycoforms rather than complex glycoforms at Asn85.
31136099	8	16	theme	Cox	1357:1359	arg1	analyses					1372:1379	stepwise Cox regression analyses	1348:1379	stepwise Cox regression analyses	1348:1379	Furthermore, stepwise Cox regression analyses revealed that HCC patients with a hybrid glycoform at Asn85 of complement C3 had a lower postsurgery tumor recurrence rate or mortality rate than those with a low amount of complement C3 protein.
31136099	5	17	theme	apolipoprotein	833:846	arg1	C-III					848:852	apolipoprotein C-III	833:852	apolipoprotein C-III	833:852	The levels of four essential proteins, including complement C3 and apolipoprotein C-III in HCC, galectin-3-binding protein in CCA, and 72 kDa inositol polyphosphate 5-phosphatase in cHCC-CCA, were highly correlated with tumor stage, tumor grade, recurrence-free survival, and overall survival.
31136099	4	18	theme	plasma	694:699	arg1	proteins					701:708	57 of 5358 identified plasma proteins	672:708	57 of 5358 identified plasma proteins	672:708	Using a mass spectrometry-based quantitative proteomic approach, we found that 57 of 5358 identified plasma proteins were differentially expressed in hepatobiliary cancers.
31136099	5	19	theme	inositol	908:915	arg1	5-phosphatase					931:943	72 kDa inositol polyphosphate 5-phosphatase	901:943	72 kDa inositol polyphosphate 5-phosphatase in cHCC-CCA	901:955	The levels of four essential proteins, including complement C3 and apolipoprotein C-III in HCC, galectin-3-binding protein in CCA, and 72 kDa inositol polyphosphate 5-phosphatase in cHCC-CCA, were highly correlated with tumor stage, tumor grade, recurrence-free survival, and overall survival.
31136099	7	20	theme	C3	1248:1249	arg1	abundance					1224:1232	The abundance	1220:1232	The abundance of complement C3 with mannose-5 or mannose-6 glycoform at Asn85	1220:1296	The abundance of complement C3 with mannose-5 or mannose-6 glycoform at Asn85 was associated with HCC tumor grade.
31136099	5	21	theme	tumor	986:990	arg1	stage					992:996	tumor stage	986:996	tumor stage	986:996	The levels of four essential proteins, including complement C3 and apolipoprotein C-III in HCC, galectin-3-binding protein in CCA, and 72 kDa inositol polyphosphate 5-phosphatase in cHCC-CCA, were highly correlated with tumor stage, tumor grade, recurrence-free survival, and overall survival.
31136099	3	22	theme	new	405:407	arg1	markers					409:415	new markers	405:415	new markers	405:415	As the majority of circulating N-glycoproteins are originated from the hepatobiliary system, we sought to explore new markers by assessing the dynamics of N-glycoproteome in plasma samples from patients with hepatocellular carcinoma (HCC), cholangiocarcinoma (CCA), or combined HCC and CCA (cHCC-CCA).
31136099	8	23	theme	complement	1554:1563	arg1	protein					1568:1574	complement C3 protein	1554:1574	complement C3 protein	1554:1574	Furthermore, stepwise Cox regression analyses revealed that HCC patients with a hybrid glycoform at Asn85 of complement C3 had a lower postsurgery tumor recurrence rate or mortality rate than those with a low amount of complement C3 protein.
31136099	4	24	theme	hepatobiliary	743:755	arg1	cancers					757:763	hepatobiliary cancers	743:763	hepatobiliary cancers	743:763	Using a mass spectrometry-based quantitative proteomic approach, we found that 57 of 5358 identified plasma proteins were differentially expressed in hepatobiliary cancers.
31136099	6	25	theme	N-glycan	1088:1095	arg1	analyses					1097:1104	Postproteomic site-specific N-glycan analyses	1060:1104	Postproteomic site-specific N-glycan analyses	1060:1104	Postproteomic site-specific N-glycan analyses showed that human complement C3 bears high-mannose and hybrid glycoforms rather than complex glycoforms at Asn85.
31136099	8	26	theme	protein	1568:1574	arg1	protein					1568:1574	complement C3 protein	1554:1574	complement C3 protein	1554:1574	Furthermore, stepwise Cox regression analyses revealed that HCC patients with a hybrid glycoform at Asn85 of complement C3 had a lower postsurgery tumor recurrence rate or mortality rate than those with a low amount of complement C3 protein.
31136099	8	26	theme	protein	1568:1574	arg1	amount					1544:1549	a low amount	1538:1549	a low amount of complement C3 protein	1538:1574	Furthermore, stepwise Cox regression analyses revealed that HCC patients with a hybrid glycoform at Asn85 of complement C3 had a lower postsurgery tumor recurrence rate or mortality rate than those with a low amount of complement C3 protein.
31136099	0	27	theme	plus	16:19	arg1	N-glycoprofiling					35:50	plus site-specific N-glycoprofiling	16:50	plus site-specific N-glycoprofiling for hepatobiliary carcinomas	16:79	Plasma proteome plus site-specific N-glycoprofiling for hepatobiliary carcinomas.
31136099	6	28	theme	Postproteomic	1060:1072	arg1	analyses					1097:1104	Postproteomic site-specific N-glycan analyses	1060:1104	Postproteomic site-specific N-glycan analyses	1060:1104	Postproteomic site-specific N-glycan analyses showed that human complement C3 bears high-mannose and hybrid glycoforms rather than complex glycoforms at Asn85.
31136099	5	29	theme	proteins	795:802	arg1	levels					770:775	The levels	766:775	The levels of four essential proteins, including complement C3 and apolipoprotein C-III in HCC, galectin-3-binding protein in CCA, and 72 kDa inositol polyphosphate 5-phosphatase in cHCC-CCA,	766:956	The levels of four essential proteins, including complement C3 and apolipoprotein C-III in HCC, galectin-3-binding protein in CCA, and 72 kDa inositol polyphosphate 5-phosphatase in cHCC-CCA, were highly correlated with tumor stage, tumor grade, recurrence-free survival, and overall survival.
31136099	9	30	theme	particular	1611:1620	arg1	N-glycoproteins					1629:1643	particular plasma N-glycoproteins	1611:1643	particular plasma N-glycoproteins with specific N-glycan compositions	1611:1679	In conclusion, our data show that particular plasma N-glycoproteins with specific N-glycan compositions could be potential noninvasive markers to evaluate oncological status and prognosis of hepatobiliary cancers.
31136099	2	31	theme	early	181:185	arg1	diagnosis					187:195	early diagnosis	181:195	early diagnosis	181:195	Appropriate markers for early diagnosis, monitoring of disease progression, and prediction of postsurgical outcome are still lacking.
31136099	8	32	theme	complement	1444:1453	arg1	C3					1455:1456	complement C3	1444:1456	complement C3	1444:1456	Furthermore, stepwise Cox regression analyses revealed that HCC patients with a hybrid glycoform at Asn85 of complement C3 had a lower postsurgery tumor recurrence rate or mortality rate than those with a low amount of complement C3 protein.
31136099	0	33	theme	hepatobiliary	56:68	arg1	carcinomas					70:79	hepatobiliary carcinomas	56:79	hepatobiliary carcinomas	56:79	Plasma proteome plus site-specific N-glycoprofiling for hepatobiliary carcinomas.
31136099	5	34	theme	overall	1042:1048	arg1	survival					1050:1057	overall survival	1042:1057	overall survival	1042:1057	The levels of four essential proteins, including complement C3 and apolipoprotein C-III in HCC, galectin-3-binding protein in CCA, and 72 kDa inositol polyphosphate 5-phosphatase in cHCC-CCA, were highly correlated with tumor stage, tumor grade, recurrence-free survival, and overall survival.
31136099	7	35	theme	tumor	1322:1326	arg1	grade					1328:1332	HCC tumor grade	1318:1332	HCC tumor grade	1318:1332	The abundance of complement C3 with mannose-5 or mannose-6 glycoform at Asn85 was associated with HCC tumor grade.
31136099	2	36	theme	outcome	264:270	arg1	monitoring					198:207	monitoring	198:207	monitoring of disease progression	198:230	Appropriate markers for early diagnosis, monitoring of disease progression, and prediction of postsurgical outcome are still lacking.
31136099	2	36	theme	outcome	264:270	arg1	diagnosis					187:195	early diagnosis	181:195	early diagnosis	181:195	Appropriate markers for early diagnosis, monitoring of disease progression, and prediction of postsurgical outcome are still lacking.
31136099	2	36	theme	outcome	264:270	arg1	prediction					237:246	prediction	237:246	prediction of postsurgical outcome	237:270	Appropriate markers for early diagnosis, monitoring of disease progression, and prediction of postsurgical outcome are still lacking.
31136099	2	37	theme	progression	220:230	arg1	monitoring					198:207	monitoring	198:207	monitoring of disease progression	198:230	Appropriate markers for early diagnosis, monitoring of disease progression, and prediction of postsurgical outcome are still lacking.
31136099	2	37	theme	progression	220:230	arg1	diagnosis					187:195	early diagnosis	181:195	early diagnosis	181:195	Appropriate markers for early diagnosis, monitoring of disease progression, and prediction of postsurgical outcome are still lacking.
31136099	2	37	theme	progression	220:230	arg1	prediction					237:246	prediction	237:246	prediction of postsurgical outcome	237:270	Appropriate markers for early diagnosis, monitoring of disease progression, and prediction of postsurgical outcome are still lacking.
31136099	3	38	theme	hepatocellular	499:512	arg1	HCC					525:527	HCC	525:527	HCC	525:527	As the majority of circulating N-glycoproteins are originated from the hepatobiliary system, we sought to explore new markers by assessing the dynamics of N-glycoproteome in plasma samples from patients with hepatocellular carcinoma (HCC), cholangiocarcinoma (CCA), or combined HCC and CCA (cHCC-CCA).
31136099	3	38	theme	hepatocellular	499:512	arg1	carcinoma					514:522	hepatocellular carcinoma	499:522	hepatocellular carcinoma (HCC)	499:528	As the majority of circulating N-glycoproteins are originated from the hepatobiliary system, we sought to explore new markers by assessing the dynamics of N-glycoproteome in plasma samples from patients with hepatocellular carcinoma (HCC), cholangiocarcinoma (CCA), or combined HCC and CCA (cHCC-CCA).
31136099	9	39	theme	specific	1650:1657	arg1	compositions					1668:1679	specific N-glycan compositions	1650:1679	specific N-glycan compositions	1650:1679	In conclusion, our data show that particular plasma N-glycoproteins with specific N-glycan compositions could be potential noninvasive markers to evaluate oncological status and prognosis of hepatobiliary cancers.
31136099	6	40	theme	high-mannose	1144:1155	arg1	glycoforms					1168:1177	high-mannose and hybrid glycoforms	1144:1177	high-mannose and hybrid glycoforms rather than complex glycoforms at Asn85	1144:1217	Postproteomic site-specific N-glycan analyses showed that human complement C3 bears high-mannose and hybrid glycoforms rather than complex glycoforms at Asn85.
31136099	8	41	theme	lower	1464:1468	arg1	rate					1499:1502	a lower postsurgery tumor recurrence rate	1462:1502	a lower postsurgery tumor recurrence rate	1462:1502	Furthermore, stepwise Cox regression analyses revealed that HCC patients with a hybrid glycoform at Asn85 of complement C3 had a lower postsurgery tumor recurrence rate or mortality rate than those with a low amount of complement C3 protein.
31136099	3	42	gly	N-glycoproteins	322:336	arg1	N-glycoproteins					322:336	circulating N-glycoproteins	310:336	circulating N-glycoproteins	310:336	As the majority of circulating N-glycoproteins are originated from the hepatobiliary system, we sought to explore new markers by assessing the dynamics of N-glycoproteome in plasma samples from patients with hepatocellular carcinoma (HCC), cholangiocarcinoma (CCA), or combined HCC and CCA (cHCC-CCA).
31136099	5	43	theme	recurrence-free	1012:1026	arg1	survival					1028:1035	recurrence-free survival	1012:1035	recurrence-free survival	1012:1035	The levels of four essential proteins, including complement C3 and apolipoprotein C-III in HCC, galectin-3-binding protein in CCA, and 72 kDa inositol polyphosphate 5-phosphatase in cHCC-CCA, were highly correlated with tumor stage, tumor grade, recurrence-free survival, and overall survival.
31136099	4	44	theme	mass	601:604	arg1	approach					648:655	a mass spectrometry-based quantitative proteomic approach	599:655	a mass spectrometry-based quantitative proteomic approach	599:655	Using a mass spectrometry-based quantitative proteomic approach, we found that 57 of 5358 identified plasma proteins were differentially expressed in hepatobiliary cancers.
31136099	6	45	theme	human	1118:1122	arg1	C3					1135:1136	human complement C3	1118:1136	human complement C3	1118:1136	Postproteomic site-specific N-glycan analyses showed that human complement C3 bears high-mannose and hybrid glycoforms rather than complex glycoforms at Asn85.
31136099	9	46	theme	noninvasive	1700:1710	arg1	markers					1712:1718	potential noninvasive markers	1690:1718	potential noninvasive markers	1690:1718	In conclusion, our data show that particular plasma N-glycoproteins with specific N-glycan compositions could be potential noninvasive markers to evaluate oncological status and prognosis of hepatobiliary cancers.
31136099	7	47	from	Asn85	1292:1296	arg1	abundance					1224:1232	The abundance	1220:1232	The abundance of complement C3 with mannose-5 or mannose-6 glycoform at Asn85	1220:1296	The abundance of complement C3 with mannose-5 or mannose-6 glycoform at Asn85 was associated with HCC tumor grade.
31136099	5	48	theme	galectin-3-binding	862:879	arg1	protein					881:887	galectin-3-binding protein	862:887	galectin-3-binding protein in CCA	862:894	The levels of four essential proteins, including complement C3 and apolipoprotein C-III in HCC, galectin-3-binding protein in CCA, and 72 kDa inositol polyphosphate 5-phosphatase in cHCC-CCA, were highly correlated with tumor stage, tumor grade, recurrence-free survival, and overall survival.
31136099	9	49	theme	oncological	1732:1742	arg1	status					1744:1749	oncological status	1732:1749	oncological status	1732:1749	In conclusion, our data show that particular plasma N-glycoproteins with specific N-glycan compositions could be potential noninvasive markers to evaluate oncological status and prognosis of hepatobiliary cancers.
31136099	6	50	theme	complex	1191:1197	arg1	glycoforms					1199:1208	complex glycoforms	1191:1208	high-mannose and hybrid glycoforms rather than complex glycoforms at Asn85	1144:1217	Postproteomic site-specific N-glycan analyses showed that human complement C3 bears high-mannose and hybrid glycoforms rather than complex glycoforms at Asn85.
31136099	3	51	theme	combined	560:567	arg1	HCC					569:571	combined HCC	560:571	combined HCC	560:571	As the majority of circulating N-glycoproteins are originated from the hepatobiliary system, we sought to explore new markers by assessing the dynamics of N-glycoproteome in plasma samples from patients with hepatocellular carcinoma (HCC), cholangiocarcinoma (CCA), or combined HCC and CCA (cHCC-CCA).
31136099	3	51	theme	combined	560:567	arg1	cHCC-CCA					582:589	cHCC-CCA	582:589	cHCC-CCA	582:589	As the majority of circulating N-glycoproteins are originated from the hepatobiliary system, we sought to explore new markers by assessing the dynamics of N-glycoproteome in plasma samples from patients with hepatocellular carcinoma (HCC), cholangiocarcinoma (CCA), or combined HCC and CCA (cHCC-CCA).
31136099	8	52	theme	tumor	1482:1486	arg1	rate					1499:1502	a lower postsurgery tumor recurrence rate	1462:1502	a lower postsurgery tumor recurrence rate	1462:1502	Furthermore, stepwise Cox regression analyses revealed that HCC patients with a hybrid glycoform at Asn85 of complement C3 had a lower postsurgery tumor recurrence rate or mortality rate than those with a low amount of complement C3 protein.
31136099	4	53	theme	identified	683:692	arg1	proteins					701:708	57 of 5358 identified plasma proteins	672:708	57 of 5358 identified plasma proteins	672:708	Using a mass spectrometry-based quantitative proteomic approach, we found that 57 of 5358 identified plasma proteins were differentially expressed in hepatobiliary cancers.
31136099	4	54	theme	quantitative	625:636	arg1	approach					648:655	a mass spectrometry-based quantitative proteomic approach	599:655	a mass spectrometry-based quantitative proteomic approach	599:655	Using a mass spectrometry-based quantitative proteomic approach, we found that 57 of 5358 identified plasma proteins were differentially expressed in hepatobiliary cancers.
31136099	6	55	theme	hybrid	1161:1166	arg1	glycoforms					1168:1177	high-mannose and hybrid glycoforms	1144:1177	high-mannose and hybrid glycoforms rather than complex glycoforms at Asn85	1144:1217	Postproteomic site-specific N-glycan analyses showed that human complement C3 bears high-mannose and hybrid glycoforms rather than complex glycoforms at Asn85.
31136099	3	56	with	patients	485:492	arg1	cholangiocarcinoma					531:548	cholangiocarcinoma	531:548	cholangiocarcinoma (CCA)	531:554	As the majority of circulating N-glycoproteins are originated from the hepatobiliary system, we sought to explore new markers by assessing the dynamics of N-glycoproteome in plasma samples from patients with hepatocellular carcinoma (HCC), cholangiocarcinoma (CCA), or combined HCC and CCA (cHCC-CCA).
31136099	3	56	with	patients	485:492	arg1	HCC					569:571	combined HCC	560:571	combined HCC	560:571	As the majority of circulating N-glycoproteins are originated from the hepatobiliary system, we sought to explore new markers by assessing the dynamics of N-glycoproteome in plasma samples from patients with hepatocellular carcinoma (HCC), cholangiocarcinoma (CCA), or combined HCC and CCA (cHCC-CCA).
31136099	3	56	with	patients	485:492	arg1	carcinoma					514:522	hepatocellular carcinoma	499:522	hepatocellular carcinoma (HCC)	499:528	As the majority of circulating N-glycoproteins are originated from the hepatobiliary system, we sought to explore new markers by assessing the dynamics of N-glycoproteome in plasma samples from patients with hepatocellular carcinoma (HCC), cholangiocarcinoma (CCA), or combined HCC and CCA (cHCC-CCA).
31136099	3	56	with	patients	485:492	arg1	CCA					551:553	CCA	551:553	CCA	551:553	As the majority of circulating N-glycoproteins are originated from the hepatobiliary system, we sought to explore new markers by assessing the dynamics of N-glycoproteome in plasma samples from patients with hepatocellular carcinoma (HCC), cholangiocarcinoma (CCA), or combined HCC and CCA (cHCC-CCA).
31136099	3	56	with	patients	485:492	arg1	HCC					525:527	HCC	525:527	HCC	525:527	As the majority of circulating N-glycoproteins are originated from the hepatobiliary system, we sought to explore new markers by assessing the dynamics of N-glycoproteome in plasma samples from patients with hepatocellular carcinoma (HCC), cholangiocarcinoma (CCA), or combined HCC and CCA (cHCC-CCA).
31136099	3	56	with	patients	485:492	arg1	cHCC-CCA					582:589	cHCC-CCA	582:589	cHCC-CCA	582:589	As the majority of circulating N-glycoproteins are originated from the hepatobiliary system, we sought to explore new markers by assessing the dynamics of N-glycoproteome in plasma samples from patients with hepatocellular carcinoma (HCC), cholangiocarcinoma (CCA), or combined HCC and CCA (cHCC-CCA).
31136099	2	57	theme	postsurgical	251:262	arg1	outcome					264:270	postsurgical outcome	251:270	postsurgical outcome	251:270	Appropriate markers for early diagnosis, monitoring of disease progression, and prediction of postsurgical outcome are still lacking.
31136099	5	58	theme	polyphosphate	917:929	arg1	5-phosphatase					931:943	72 kDa inositol polyphosphate 5-phosphatase	901:943	72 kDa inositol polyphosphate 5-phosphatase in cHCC-CCA	901:955	The levels of four essential proteins, including complement C3 and apolipoprotein C-III in HCC, galectin-3-binding protein in CCA, and 72 kDa inositol polyphosphate 5-phosphatase in cHCC-CCA, were highly correlated with tumor stage, tumor grade, recurrence-free survival, and overall survival.
31136099	9	59	theme	cancers	1782:1788	arg1	prognosis					1755:1763	prognosis	1755:1763	prognosis	1755:1763	In conclusion, our data show that particular plasma N-glycoproteins with specific N-glycan compositions could be potential noninvasive markers to evaluate oncological status and prognosis of hepatobiliary cancers.
31136099	9	59	theme	cancers	1782:1788	arg1	status					1744:1749	oncological status	1732:1749	oncological status	1732:1749	In conclusion, our data show that particular plasma N-glycoproteins with specific N-glycan compositions could be potential noninvasive markers to evaluate oncological status and prognosis of hepatobiliary cancers.
31136099	8	60	theme	mortality	1507:1515	arg1	rate					1517:1520	mortality rate	1507:1520	mortality rate	1507:1520	Furthermore, stepwise Cox regression analyses revealed that HCC patients with a hybrid glycoform at Asn85 of complement C3 had a lower postsurgery tumor recurrence rate or mortality rate than those with a low amount of complement C3 protein.
31136099	1	61	theme	death	140:144	arg1	worldwide					146:154	cancer death worldwide	133:154	cancer death worldwide	133:154	Hepatobiliary cancer is the third leading cause of cancer death worldwide.
31136099	3	62	theme	circulating	310:320	arg1	N-glycoproteins					322:336	circulating N-glycoproteins	310:336	circulating N-glycoproteins	310:336	As the majority of circulating N-glycoproteins are originated from the hepatobiliary system, we sought to explore new markers by assessing the dynamics of N-glycoproteome in plasma samples from patients with hepatocellular carcinoma (HCC), cholangiocarcinoma (CCA), or combined HCC and CCA (cHCC-CCA).
31136099	3	63	theme	hepatobiliary	362:374	arg1	system					376:381	the hepatobiliary system	358:381	the hepatobiliary system	358:381	As the majority of circulating N-glycoproteins are originated from the hepatobiliary system, we sought to explore new markers by assessing the dynamics of N-glycoproteome in plasma samples from patients with hepatocellular carcinoma (HCC), cholangiocarcinoma (CCA), or combined HCC and CCA (cHCC-CCA).
31136099	8	64	theme	stepwise	1348:1355	arg1	analyses					1372:1379	stepwise Cox regression analyses	1348:1379	stepwise Cox regression analyses	1348:1379	Furthermore, stepwise Cox regression analyses revealed that HCC patients with a hybrid glycoform at Asn85 of complement C3 had a lower postsurgery tumor recurrence rate or mortality rate than those with a low amount of complement C3 protein.
31136099	5	65	from	protein	881:887	arg1	HCC					857:859	HCC	857:859	HCC	857:859	The levels of four essential proteins, including complement C3 and apolipoprotein C-III in HCC, galectin-3-binding protein in CCA, and 72 kDa inositol polyphosphate 5-phosphatase in cHCC-CCA, were highly correlated with tumor stage, tumor grade, recurrence-free survival, and overall survival.
31136099	5	65	from	protein	881:887	arg1	cHCC-CCA					948:955	cHCC-CCA	948:955	cHCC-CCA	948:955	The levels of four essential proteins, including complement C3 and apolipoprotein C-III in HCC, galectin-3-binding protein in CCA, and 72 kDa inositol polyphosphate 5-phosphatase in cHCC-CCA, were highly correlated with tumor stage, tumor grade, recurrence-free survival, and overall survival.
31136099	5	65	from	protein	881:887	arg1	CCA					892:894	CCA	892:894	CCA	892:894	The levels of four essential proteins, including complement C3 and apolipoprotein C-III in HCC, galectin-3-binding protein in CCA, and 72 kDa inositol polyphosphate 5-phosphatase in cHCC-CCA, were highly correlated with tumor stage, tumor grade, recurrence-free survival, and overall survival.
31136099	5	66	theme	72 kDa	901:906	arg1	5-phosphatase					931:943	72 kDa inositol polyphosphate 5-phosphatase	901:943	72 kDa inositol polyphosphate 5-phosphatase in cHCC-CCA	901:955	The levels of four essential proteins, including complement C3 and apolipoprotein C-III in HCC, galectin-3-binding protein in CCA, and 72 kDa inositol polyphosphate 5-phosphatase in cHCC-CCA, were highly correlated with tumor stage, tumor grade, recurrence-free survival, and overall survival.
31136099	8	67	theme	low	1540:1542	arg1	protein					1568:1574	complement C3 protein	1554:1574	complement C3 protein	1554:1574	Furthermore, stepwise Cox regression analyses revealed that HCC patients with a hybrid glycoform at Asn85 of complement C3 had a lower postsurgery tumor recurrence rate or mortality rate than those with a low amount of complement C3 protein.
31136099	8	67	theme	low	1540:1542	arg1	amount					1544:1549	a low amount	1538:1549	a low amount of complement C3 protein	1538:1574	Furthermore, stepwise Cox regression analyses revealed that HCC patients with a hybrid glycoform at Asn85 of complement C3 had a lower postsurgery tumor recurrence rate or mortality rate than those with a low amount of complement C3 protein.
31136099	8	68	theme	regression	1361:1370	arg1	analyses					1372:1379	stepwise Cox regression analyses	1348:1379	stepwise Cox regression analyses	1348:1379	Furthermore, stepwise Cox regression analyses revealed that HCC patients with a hybrid glycoform at Asn85 of complement C3 had a lower postsurgery tumor recurrence rate or mortality rate than those with a low amount of complement C3 protein.
31136099	7	69	theme	complement	1237:1246	arg1	C3					1248:1249	complement C3	1237:1249	complement C3	1237:1249	The abundance of complement C3 with mannose-5 or mannose-6 glycoform at Asn85 was associated with HCC tumor grade.
31136099	1	70	theme	Hepatobiliary	82:94	arg1	cancer					96:101	Hepatobiliary cancer	82:101	Hepatobiliary cancer	82:101	Hepatobiliary cancer is the third leading cause of cancer death worldwide.
31136099	1	70	theme	Hepatobiliary	82:94	arg1	cause					124:128	the third leading cause	106:128	the third leading cause of cancer death worldwide	106:154	Hepatobiliary cancer is the third leading cause of cancer death worldwide.
31136099	6	71	from	Asn85	1213:1217	arg1	glycoforms					1199:1208	complex glycoforms	1191:1208	high-mannose and hybrid glycoforms rather than complex glycoforms at Asn85	1144:1217	Postproteomic site-specific N-glycan analyses showed that human complement C3 bears high-mannose and hybrid glycoforms rather than complex glycoforms at Asn85.
31136099	8	72	theme	C3	1565:1566	arg1	protein					1568:1574	complement C3 protein	1554:1574	complement C3 protein	1554:1574	Furthermore, stepwise Cox regression analyses revealed that HCC patients with a hybrid glycoform at Asn85 of complement C3 had a lower postsurgery tumor recurrence rate or mortality rate than those with a low amount of complement C3 protein.
31136099	6	73	gly	glycoforms	1168:1177	arg1	hybrid					1161:1166	hybrid	1161:1166	hybrid	1161:1166	Postproteomic site-specific N-glycan analyses showed that human complement C3 bears high-mannose and hybrid glycoforms rather than complex glycoforms at Asn85.
31136099	6	73	gly	glycoforms	1168:1177	arg1	high-mannose					1144:1155	high-mannose	1144:1155	high-mannose	1144:1155	Postproteomic site-specific N-glycan analyses showed that human complement C3 bears high-mannose and hybrid glycoforms rather than complex glycoforms at Asn85.
31136099	8	74	theme	HCC	1395:1397	arg1	patients					1399:1406	HCC patients	1395:1406	HCC patients with a hybrid glycoform at Asn85 of complement C3	1395:1456	Furthermore, stepwise Cox regression analyses revealed that HCC patients with a hybrid glycoform at Asn85 of complement C3 had a lower postsurgery tumor recurrence rate or mortality rate than those with a low amount of complement C3 protein.
31136099	3	75	theme	plasma	465:470	arg1	samples					472:478	plasma samples	465:478	plasma samples from patients with hepatocellular carcinoma (HCC), cholangiocarcinoma (CCA), or combined HCC and CCA (cHCC-CCA)	465:590	As the majority of circulating N-glycoproteins are originated from the hepatobiliary system, we sought to explore new markers by assessing the dynamics of N-glycoproteome in plasma samples from patients with hepatocellular carcinoma (HCC), cholangiocarcinoma (CCA), or combined HCC and CCA (cHCC-CCA).
31136099	5	76	theme	complement	815:824	arg1	C3					826:827	complement C3	815:827	complement C3	815:827	The levels of four essential proteins, including complement C3 and apolipoprotein C-III in HCC, galectin-3-binding protein in CCA, and 72 kDa inositol polyphosphate 5-phosphatase in cHCC-CCA, were highly correlated with tumor stage, tumor grade, recurrence-free survival, and overall survival.
31136099	5	77	from	C3	826:827	arg1	HCC					857:859	HCC	857:859	HCC	857:859	The levels of four essential proteins, including complement C3 and apolipoprotein C-III in HCC, galectin-3-binding protein in CCA, and 72 kDa inositol polyphosphate 5-phosphatase in cHCC-CCA, were highly correlated with tumor stage, tumor grade, recurrence-free survival, and overall survival.
31136099	5	77	from	C3	826:827	arg1	cHCC-CCA					948:955	cHCC-CCA	948:955	cHCC-CCA	948:955	The levels of four essential proteins, including complement C3 and apolipoprotein C-III in HCC, galectin-3-binding protein in CCA, and 72 kDa inositol polyphosphate 5-phosphatase in cHCC-CCA, were highly correlated with tumor stage, tumor grade, recurrence-free survival, and overall survival.
31136099	5	77	from	C3	826:827	arg1	CCA					892:894	CCA	892:894	CCA	892:894	The levels of four essential proteins, including complement C3 and apolipoprotein C-III in HCC, galectin-3-binding protein in CCA, and 72 kDa inositol polyphosphate 5-phosphatase in cHCC-CCA, were highly correlated with tumor stage, tumor grade, recurrence-free survival, and overall survival.
31136099	3	78	theme	N-glycoproteome	446:460	arg1	dynamics					434:441	the dynamics	430:441	the dynamics of N-glycoproteome	430:460	As the majority of circulating N-glycoproteins are originated from the hepatobiliary system, we sought to explore new markers by assessing the dynamics of N-glycoproteome in plasma samples from patients with hepatocellular carcinoma (HCC), cholangiocarcinoma (CCA), or combined HCC and CCA (cHCC-CCA).
31136099	0	79	theme	site-specific	21:33	arg1	N-glycoprofiling					35:50	plus site-specific N-glycoprofiling	16:50	plus site-specific N-glycoprofiling for hepatobiliary carcinomas	16:79	Plasma proteome plus site-specific N-glycoprofiling for hepatobiliary carcinomas.
31136099	5	80	from	5-phosphatase	931:943	arg1	HCC					857:859	HCC	857:859	HCC	857:859	The levels of four essential proteins, including complement C3 and apolipoprotein C-III in HCC, galectin-3-binding protein in CCA, and 72 kDa inositol polyphosphate 5-phosphatase in cHCC-CCA, were highly correlated with tumor stage, tumor grade, recurrence-free survival, and overall survival.
31136099	5	80	from	5-phosphatase	931:943	arg1	cHCC-CCA					948:955	cHCC-CCA	948:955	cHCC-CCA	948:955	The levels of four essential proteins, including complement C3 and apolipoprotein C-III in HCC, galectin-3-binding protein in CCA, and 72 kDa inositol polyphosphate 5-phosphatase in cHCC-CCA, were highly correlated with tumor stage, tumor grade, recurrence-free survival, and overall survival.
31136099	5	80	from	5-phosphatase	931:943	arg1	CCA					892:894	CCA	892:894	CCA	892:894	The levels of four essential proteins, including complement C3 and apolipoprotein C-III in HCC, galectin-3-binding protein in CCA, and 72 kDa inositol polyphosphate 5-phosphatase in cHCC-CCA, were highly correlated with tumor stage, tumor grade, recurrence-free survival, and overall survival.
31136099	1	81	theme	leading	116:122	arg1	cancer					96:101	Hepatobiliary cancer	82:101	Hepatobiliary cancer	82:101	Hepatobiliary cancer is the third leading cause of cancer death worldwide.
31136099	1	81	theme	leading	116:122	arg1	cause					124:128	the third leading cause	106:128	the third leading cause of cancer death worldwide	106:154	Hepatobiliary cancer is the third leading cause of cancer death worldwide.
31136099	6	82	theme	site-specific	1074:1086	arg1	analyses					1097:1104	Postproteomic site-specific N-glycan analyses	1060:1104	Postproteomic site-specific N-glycan analyses	1060:1104	Postproteomic site-specific N-glycan analyses showed that human complement C3 bears high-mannose and hybrid glycoforms rather than complex glycoforms at Asn85.
31136099	8	83	contain	had	1458:1460	arg1	patients					1399:1406	HCC patients	1395:1406	HCC patients with a hybrid glycoform at Asn85 of complement C3	1395:1456	Furthermore, stepwise Cox regression analyses revealed that HCC patients with a hybrid glycoform at Asn85 of complement C3 had a lower postsurgery tumor recurrence rate or mortality rate than those with a low amount of complement C3 protein.
31136099	8	83	contain	had	1458:1460	arg2	rate					1499:1502	a lower postsurgery tumor recurrence rate	1462:1502	a lower postsurgery tumor recurrence rate	1462:1502	Furthermore, stepwise Cox regression analyses revealed that HCC patients with a hybrid glycoform at Asn85 of complement C3 had a lower postsurgery tumor recurrence rate or mortality rate than those with a low amount of complement C3 protein.
31136099	8	83	contain	had	1458:1460	arg2	rate					1517:1520	mortality rate	1507:1520	mortality rate	1507:1520	Furthermore, stepwise Cox regression analyses revealed that HCC patients with a hybrid glycoform at Asn85 of complement C3 had a lower postsurgery tumor recurrence rate or mortality rate than those with a low amount of complement C3 protein.
31136099	8	84	theme	hybrid	1415:1420	arg1	glycoform					1422:1430	a hybrid glycoform	1413:1430	a hybrid glycoform at Asn85 of complement C3	1413:1456	Furthermore, stepwise Cox regression analyses revealed that HCC patients with a hybrid glycoform at Asn85 of complement C3 had a lower postsurgery tumor recurrence rate or mortality rate than those with a low amount of complement C3 protein.
31136099	5	85	theme	essential	785:793	arg1	protein					881:887	galectin-3-binding protein	862:887	galectin-3-binding protein in CCA	862:894	The levels of four essential proteins, including complement C3 and apolipoprotein C-III in HCC, galectin-3-binding protein in CCA, and 72 kDa inositol polyphosphate 5-phosphatase in cHCC-CCA, were highly correlated with tumor stage, tumor grade, recurrence-free survival, and overall survival.
31136099	5	85	theme	essential	785:793	arg1	C-III					848:852	apolipoprotein C-III	833:852	apolipoprotein C-III	833:852	The levels of four essential proteins, including complement C3 and apolipoprotein C-III in HCC, galectin-3-binding protein in CCA, and 72 kDa inositol polyphosphate 5-phosphatase in cHCC-CCA, were highly correlated with tumor stage, tumor grade, recurrence-free survival, and overall survival.
31136099	5	85	theme	essential	785:793	arg1	5-phosphatase					931:943	72 kDa inositol polyphosphate 5-phosphatase	901:943	72 kDa inositol polyphosphate 5-phosphatase in cHCC-CCA	901:955	The levels of four essential proteins, including complement C3 and apolipoprotein C-III in HCC, galectin-3-binding protein in CCA, and 72 kDa inositol polyphosphate 5-phosphatase in cHCC-CCA, were highly correlated with tumor stage, tumor grade, recurrence-free survival, and overall survival.
31136099	5	85	theme	essential	785:793	arg1	proteins					795:802	four essential proteins	780:802	four essential proteins	780:802	The levels of four essential proteins, including complement C3 and apolipoprotein C-III in HCC, galectin-3-binding protein in CCA, and 72 kDa inositol polyphosphate 5-phosphatase in cHCC-CCA, were highly correlated with tumor stage, tumor grade, recurrence-free survival, and overall survival.
31136099	5	85	theme	essential	785:793	arg1	C3					826:827	complement C3	815:827	complement C3	815:827	The levels of four essential proteins, including complement C3 and apolipoprotein C-III in HCC, galectin-3-binding protein in CCA, and 72 kDa inositol polyphosphate 5-phosphatase in cHCC-CCA, were highly correlated with tumor stage, tumor grade, recurrence-free survival, and overall survival.
31136099	4	86	theme	spectrometry-based	606:623	arg1	approach					648:655	a mass spectrometry-based quantitative proteomic approach	599:655	a mass spectrometry-based quantitative proteomic approach	599:655	Using a mass spectrometry-based quantitative proteomic approach, we found that 57 of 5358 identified plasma proteins were differentially expressed in hepatobiliary cancers.
31136099	9	87	theme	plasma	1622:1627	arg1	N-glycoproteins					1629:1643	particular plasma N-glycoproteins	1611:1643	particular plasma N-glycoproteins with specific N-glycan compositions	1611:1679	In conclusion, our data show that particular plasma N-glycoproteins with specific N-glycan compositions could be potential noninvasive markers to evaluate oncological status and prognosis of hepatobiliary cancers.
31136099	7	88	theme	HCC	1318:1320	arg1	grade					1328:1332	HCC tumor grade	1318:1332	HCC tumor grade	1318:1332	The abundance of complement C3 with mannose-5 or mannose-6 glycoform at Asn85 was associated with HCC tumor grade.
31136099	3	89	from	patients	485:492	arg1	samples					472:478	plasma samples	465:478	plasma samples from patients with hepatocellular carcinoma (HCC), cholangiocarcinoma (CCA), or combined HCC and CCA (cHCC-CCA)	465:590	As the majority of circulating N-glycoproteins are originated from the hepatobiliary system, we sought to explore new markers by assessing the dynamics of N-glycoproteome in plasma samples from patients with hepatocellular carcinoma (HCC), cholangiocarcinoma (CCA), or combined HCC and CCA (cHCC-CCA).
31136099	8	90	gly	glycoform	1422:1430	arg1	hybrid					1415:1420	a hybrid glycoform	1413:1430	a hybrid glycoform at Asn85 of complement C3	1413:1456	Furthermore, stepwise Cox regression analyses revealed that HCC patients with a hybrid glycoform at Asn85 of complement C3 had a lower postsurgery tumor recurrence rate or mortality rate than those with a low amount of complement C3 protein.
31136099	5	91	theme	tumor	999:1003	arg1	grade					1005:1009	tumor grade	999:1009	tumor grade	999:1009	The levels of four essential proteins, including complement C3 and apolipoprotein C-III in HCC, galectin-3-binding protein in CCA, and 72 kDa inositol polyphosphate 5-phosphatase in cHCC-CCA, were highly correlated with tumor stage, tumor grade, recurrence-free survival, and overall survival.
31136099	2	92	theme	disease	212:218	arg1	progression					220:230	disease progression	212:230	disease progression	212:230	Appropriate markers for early diagnosis, monitoring of disease progression, and prediction of postsurgical outcome are still lacking.
31136099	8	93	theme	C3	1455:1456	arg1	Asn85					1435:1439	Asn85	1435:1439	Asn85	1435:1439	Furthermore, stepwise Cox regression analyses revealed that HCC patients with a hybrid glycoform at Asn85 of complement C3 had a lower postsurgery tumor recurrence rate or mortality rate than those with a low amount of complement C3 protein.
31136099	8	93	theme	C3	1455:1456	arg1	C3					1455:1456	complement C3	1444:1456	complement C3	1444:1456	Furthermore, stepwise Cox regression analyses revealed that HCC patients with a hybrid glycoform at Asn85 of complement C3 had a lower postsurgery tumor recurrence rate or mortality rate than those with a low amount of complement C3 protein.
31136099	9	94	theme	N-glycan	1659:1666	arg1	compositions					1668:1679	specific N-glycan compositions	1650:1679	specific N-glycan compositions	1650:1679	In conclusion, our data show that particular plasma N-glycoproteins with specific N-glycan compositions could be potential noninvasive markers to evaluate oncological status and prognosis of hepatobiliary cancers.
31136099	9	95	gly	N-glycoproteins	1629:1643	arg1	N-glycoproteins					1629:1643	particular plasma N-glycoproteins	1611:1643	particular plasma N-glycoproteins with specific N-glycan compositions	1611:1679	In conclusion, our data show that particular plasma N-glycoproteins with specific N-glycan compositions could be potential noninvasive markers to evaluate oncological status and prognosis of hepatobiliary cancers.
30377844	0	0	theme	LPS-stimulated	97:110	arg1	cells					118:122	LPS-stimulated THP-1 cells	97:122	LPS-stimulated THP-1 cells	97:122	Isolation of polysaccharides from Dendrobium officinale leaves and anti-inflammatory activity in LPS-stimulated THP-1 cells.
30377844	6	1	theme	-mannose	866:873	arg1	D-					734:735	D-	734:735	D-	734:735	DLP-1 was composed of D-(+)-galactose, DL-arabinose, and L-(+)-rhamnose with a molar ratio of 3.21:1.11:0.23, and traces of D-xylose, D-glucose, and D-(+)-mannose.
30377844	10	2	from	factor	1466:1471	arg1	cells					1598:1602	LPS-stimulated THP-1 cells	1577:1602	LPS-stimulated THP-1 cells	1577:1602	In addition, both DLP-1 (5 μg/mL) and DLP-2 (50 μg/mL) significantly suppressed toll-like receptor-4 (TLR-4), myeloid differentiation factor (MyD88) and tumour necrosis factor receptor-associated factor-6 (TRAF-6) mRNA and protein expression in LPS-stimulated THP-1 cells.
30377844	5	3	theme	gas	674:676	arg1	spectrometer					698:709	gas chromatography-mass spectrometer	674:709	gas chromatography-mass spectrometer	674:709	Monosaccharide compositions were analyzed using gas chromatography-mass spectrometer.
30377844	10	4	theme	THP-1	1592:1596	arg1	cells					1598:1602	LPS-stimulated THP-1 cells	1577:1602	LPS-stimulated THP-1 cells	1577:1602	In addition, both DLP-1 (5 μg/mL) and DLP-2 (50 μg/mL) significantly suppressed toll-like receptor-4 (TLR-4), myeloid differentiation factor (MyD88) and tumour necrosis factor receptor-associated factor-6 (TRAF-6) mRNA and protein expression in LPS-stimulated THP-1 cells.
30377844	6	5	theme	D-xylose	836:843	arg1	D-					861:862	D-	861:862	D-	861:862	DLP-1 was composed of D-(+)-galactose, DL-arabinose, and L-(+)-rhamnose with a molar ratio of 3.21:1.11:0.23, and traces of D-xylose, D-glucose, and D-(+)-mannose.
30377844	6	5	theme	D-xylose	836:843	arg1	traces					826:831	traces	826:831	traces of D-xylose	826:843	DLP-1 was composed of D-(+)-galactose, DL-arabinose, and L-(+)-rhamnose with a molar ratio of 3.21:1.11:0.23, and traces of D-xylose, D-glucose, and D-(+)-mannose.
30377844	6	5	theme	D-xylose	836:843	arg1	L-					769:770	L-	769:770	L-	769:770	DLP-1 was composed of D-(+)-galactose, DL-arabinose, and L-(+)-rhamnose with a molar ratio of 3.21:1.11:0.23, and traces of D-xylose, D-glucose, and D-(+)-mannose.
30377844	6	5	theme	D-xylose	836:843	arg1	D-glucose					846:854	D-glucose	846:854	D-glucose	846:854	DLP-1 was composed of D-(+)-galactose, DL-arabinose, and L-(+)-rhamnose with a molar ratio of 3.21:1.11:0.23, and traces of D-xylose, D-glucose, and D-(+)-mannose.
30377844	7	6	theme	-galactose	918:927	arg1	D-glucose					899:907	D-glucose	899:907	D-glucose	899:907	DLP-2 was consisted of D-glucose and D-(+)-galactose with a molar ratio of 3.23:1.02, and traces of D-xylose, DL-arabinose.
30377844	8	7	theme	anti-inflammatory	1090:1106	arg1	effects					1108:1114	the anti-inflammatory effects	1086:1114	the anti-inflammatory effects of DLP-1 and DLP-2	1086:1133	Then, we established inflammatory cell model by LPS acting THP-1 cells to investigate the anti-inflammatory effects of DLP-1 and DLP-2.
30377844	0	8	from	Isolation	0:8	arg1	officinale					45:54	Dendrobium officinale	34:54	Dendrobium officinale	34:54	Isolation of polysaccharides from Dendrobium officinale leaves and anti-inflammatory activity in LPS-stimulated THP-1 cells.
30377844	5	9	theme	chromatography-mass	678:696	arg1	spectrometer					698:709	gas chromatography-mass spectrometer	674:709	gas chromatography-mass spectrometer	674:709	Monosaccharide compositions were analyzed using gas chromatography-mass spectrometer.
30377844	6	10	theme	molar	791:795	arg1	ratio					797:801	a molar ratio	789:801	a molar ratio of 3.21:1.11:0.23	789:819	DLP-1 was composed of D-(+)-galactose, DL-arabinose, and L-(+)-rhamnose with a molar ratio of 3.21:1.11:0.23, and traces of D-xylose, D-glucose, and D-(+)-mannose.
30377844	3	11	dep	separation	473:482	arg1	column					529:534	Sephadex G-100 column	514:534	Sephadex G-100 column	514:534	Two kinds of polysaccharides in the leaves, DLP-1 and DLP-2, were obtained by hot water extraction, alcohol sedimentation and chromatographic separation (DEAE-52 cellulose column and Sephadex G-100 column).
30377844	3	11	dep	separation	473:482	arg1	column					503:508	DEAE-52 cellulose column	485:508	DEAE-52 cellulose column	485:508	Two kinds of polysaccharides in the leaves, DLP-1 and DLP-2, were obtained by hot water extraction, alcohol sedimentation and chromatographic separation (DEAE-52 cellulose column and Sephadex G-100 column).
30377844	2	12	from	officinale	312:321	arg1	polysaccharides					280:294	the polysaccharides	276:294	the polysaccharides from Dendrobium officinale leaves	276:328	However, little was known about the polysaccharides from Dendrobium officinale leaves.
30377844	0	13	theme	THP-1	112:116	arg1	cells					118:122	LPS-stimulated THP-1 cells	97:122	LPS-stimulated THP-1 cells	97:122	Isolation of polysaccharides from Dendrobium officinale leaves and anti-inflammatory activity in LPS-stimulated THP-1 cells.
30377844	9	14	theme	LPS-stimulated	1246:1259	arg1	cytotoxicity					1261:1272	LPS-stimulated cytotoxicity	1246:1272	LPS-stimulated cytotoxicity	1246:1272	The results indicated that DLP-1 (5 μg/mL) and DLP-2 (50 μg/mL) were effective in protecting THP-1 cells from LPS-stimulated cytotoxicity, as well as inhibiting reactive oxygen species formation.
30377844	3	15	from	the	363:365	arg1	kinds					335:339	Two kinds	331:339	Two kinds of polysaccharides in the leaves	331:372	Two kinds of polysaccharides in the leaves, DLP-1 and DLP-2, were obtained by hot water extraction, alcohol sedimentation and chromatographic separation (DEAE-52 cellulose column and Sephadex G-100 column).
30377844	3	15	from	the	363:365	arg1	DLP-2					385:389	DLP-2	385:389	DLP-2	385:389	Two kinds of polysaccharides in the leaves, DLP-1 and DLP-2, were obtained by hot water extraction, alcohol sedimentation and chromatographic separation (DEAE-52 cellulose column and Sephadex G-100 column).
30377844	3	15	from	the	363:365	arg1	DLP-1					375:379	DLP-1	375:379	DLP-1	375:379	Two kinds of polysaccharides in the leaves, DLP-1 and DLP-2, were obtained by hot water extraction, alcohol sedimentation and chromatographic separation (DEAE-52 cellulose column and Sephadex G-100 column).
30377844	3	16	theme	polysaccharides	344:358	arg1	kinds					335:339	Two kinds	331:339	Two kinds of polysaccharides in the leaves	331:372	Two kinds of polysaccharides in the leaves, DLP-1 and DLP-2, were obtained by hot water extraction, alcohol sedimentation and chromatographic separation (DEAE-52 cellulose column and Sephadex G-100 column).
30377844	3	16	theme	polysaccharides	344:358	arg1	DLP-2					385:389	DLP-2	385:389	DLP-2	385:389	Two kinds of polysaccharides in the leaves, DLP-1 and DLP-2, were obtained by hot water extraction, alcohol sedimentation and chromatographic separation (DEAE-52 cellulose column and Sephadex G-100 column).
30377844	3	16	theme	polysaccharides	344:358	arg1	DLP-1					375:379	DLP-1	375:379	DLP-1	375:379	Two kinds of polysaccharides in the leaves, DLP-1 and DLP-2, were obtained by hot water extraction, alcohol sedimentation and chromatographic separation (DEAE-52 cellulose column and Sephadex G-100 column).
30377844	1	17	theme	officinale	136:145	arg1	stem					147:150	Dendrobium officinale stem	125:150	Dendrobium officinale stem	125:150	Dendrobium officinale stem is rich in polysaccharides, which play a great role in the medicinal effects of this plant.
30377844	5	18	theme	Monosaccharide	626:639	arg1	compositions					641:652	Monosaccharide compositions	626:652	Monosaccharide compositions	626:652	Monosaccharide compositions were analyzed using gas chromatography-mass spectrometer.
30377844	1	19	from	polysaccharides	163:177	arg1	rich					155:158	rich	155:158	rich	155:158	Dendrobium officinale stem is rich in polysaccharides, which play a great role in the medicinal effects of this plant.
30377844	10	20	theme	differentiation	1450:1464	arg1	MyD88					1474:1478	MyD88	1474:1478	MyD88	1474:1478	In addition, both DLP-1 (5 μg/mL) and DLP-2 (50 μg/mL) significantly suppressed toll-like receptor-4 (TLR-4), myeloid differentiation factor (MyD88) and tumour necrosis factor receptor-associated factor-6 (TRAF-6) mRNA and protein expression in LPS-stimulated THP-1 cells.
30377844	10	20	theme	differentiation	1450:1464	arg1	factor					1466:1471	myeloid differentiation factor	1442:1471	myeloid differentiation factor (MyD88)	1442:1479	In addition, both DLP-1 (5 μg/mL) and DLP-2 (50 μg/mL) significantly suppressed toll-like receptor-4 (TLR-4), myeloid differentiation factor (MyD88) and tumour necrosis factor receptor-associated factor-6 (TRAF-6) mRNA and protein expression in LPS-stimulated THP-1 cells.
30377844	3	21	theme	hot	409:411	arg1	extraction					419:428	hot water extraction	409:428	hot water extraction	409:428	Two kinds of polysaccharides in the leaves, DLP-1 and DLP-2, were obtained by hot water extraction, alcohol sedimentation and chromatographic separation (DEAE-52 cellulose column and Sephadex G-100 column).
30377844	10	22	theme	factor-6	1528:1535	arg1	mRNA					1546:1549	tumour necrosis factor receptor-associated factor-6 (TRAF-6) mRNA	1485:1549	tumour necrosis factor receptor-associated factor-6 (TRAF-6) mRNA	1485:1549	In addition, both DLP-1 (5 μg/mL) and DLP-2 (50 μg/mL) significantly suppressed toll-like receptor-4 (TLR-4), myeloid differentiation factor (MyD88) and tumour necrosis factor receptor-associated factor-6 (TRAF-6) mRNA and protein expression in LPS-stimulated THP-1 cells.
30377844	0	23	from	officinale	45:54	arg1	polysaccharides					13:27	polysaccharides	13:27	polysaccharides from Dendrobium officinale	13:54	Isolation of polysaccharides from Dendrobium officinale leaves and anti-inflammatory activity in LPS-stimulated THP-1 cells.
30377844	0	23	from	officinale	45:54	arg1	Isolation					0:8	Isolation	0:8	Isolation of polysaccharides from Dendrobium officinale	0:54	Isolation of polysaccharides from Dendrobium officinale leaves and anti-inflammatory activity in LPS-stimulated THP-1 cells.
30377844	10	24	theme	protein	1555:1561	arg1	expression					1563:1572	tumour necrosis factor receptor-associated factor-6 (TRAF-6) mRNA and protein expression	1485:1572	tumour necrosis factor receptor-associated factor-6 (TRAF-6) mRNA and protein expression in LPS-stimulated THP-1 cells	1485:1602	In addition, both DLP-1 (5 μg/mL) and DLP-2 (50 μg/mL) significantly suppressed toll-like receptor-4 (TLR-4), myeloid differentiation factor (MyD88) and tumour necrosis factor receptor-associated factor-6 (TRAF-6) mRNA and protein expression in LPS-stimulated THP-1 cells.
30377844	10	25	theme	receptor-associated	1508:1526	arg1	mRNA					1546:1549	tumour necrosis factor receptor-associated factor-6 (TRAF-6) mRNA	1485:1549	tumour necrosis factor receptor-associated factor-6 (TRAF-6) mRNA	1485:1549	In addition, both DLP-1 (5 μg/mL) and DLP-2 (50 μg/mL) significantly suppressed toll-like receptor-4 (TLR-4), myeloid differentiation factor (MyD88) and tumour necrosis factor receptor-associated factor-6 (TRAF-6) mRNA and protein expression in LPS-stimulated THP-1 cells.
30377844	1	26	from	rich	155:158	arg1	polysaccharides					163:177	polysaccharides	163:177	polysaccharides	163:177	Dendrobium officinale stem is rich in polysaccharides, which play a great role in the medicinal effects of this plant.
30377844	9	27	theme	THP-1	1229:1233	arg1	cells					1235:1239	THP-1 cells	1229:1239	THP-1 cells	1229:1239	The results indicated that DLP-1 (5 μg/mL) and DLP-2 (50 μg/mL) were effective in protecting THP-1 cells from LPS-stimulated cytotoxicity, as well as inhibiting reactive oxygen species formation.
30377844	1	28	theme	great	193:197	arg1	role					199:202	a great role	191:202	a great role	191:202	Dendrobium officinale stem is rich in polysaccharides, which play a great role in the medicinal effects of this plant.
30377844	8	29	theme	DLP-1	1119:1123	arg1	effects					1108:1114	the anti-inflammatory effects	1086:1114	the anti-inflammatory effects of DLP-1 and DLP-2	1086:1133	Then, we established inflammatory cell model by LPS acting THP-1 cells to investigate the anti-inflammatory effects of DLP-1 and DLP-2.
30377844	4	30	theme	molecular	550:558	arg1	28,342 Da					587:595	28,342 Da	587:595	28,342 Da	587:595	The average molecular weights were determined as 28,342 Da and 41,143 Da, respectively.
30377844	4	30	theme	molecular	550:558	arg1	weights					560:566	The average molecular weights	538:566	The average molecular weights	538:566	The average molecular weights were determined as 28,342 Da and 41,143 Da, respectively.
30377844	4	30	theme	molecular	550:558	arg1	41,143 Da					601:609	41,143 Da	601:609	41,143 Da	601:609	The average molecular weights were determined as 28,342 Da and 41,143 Da, respectively.
30377844	10	31	theme	myeloid	1442:1448	arg1	MyD88					1474:1478	MyD88	1474:1478	MyD88	1474:1478	In addition, both DLP-1 (5 μg/mL) and DLP-2 (50 μg/mL) significantly suppressed toll-like receptor-4 (TLR-4), myeloid differentiation factor (MyD88) and tumour necrosis factor receptor-associated factor-6 (TRAF-6) mRNA and protein expression in LPS-stimulated THP-1 cells.
30377844	10	31	theme	myeloid	1442:1448	arg1	factor					1466:1471	myeloid differentiation factor	1442:1471	myeloid differentiation factor (MyD88)	1442:1479	In addition, both DLP-1 (5 μg/mL) and DLP-2 (50 μg/mL) significantly suppressed toll-like receptor-4 (TLR-4), myeloid differentiation factor (MyD88) and tumour necrosis factor receptor-associated factor-6 (TRAF-6) mRNA and protein expression in LPS-stimulated THP-1 cells.
30377844	3	32	theme	water	413:417	arg1	extraction					419:428	hot water extraction	409:428	hot water extraction	409:428	Two kinds of polysaccharides in the leaves, DLP-1 and DLP-2, were obtained by hot water extraction, alcohol sedimentation and chromatographic separation (DEAE-52 cellulose column and Sephadex G-100 column).
30377844	4	33	theme	average	542:548	arg1	28,342 Da					587:595	28,342 Da	587:595	28,342 Da	587:595	The average molecular weights were determined as 28,342 Da and 41,143 Da, respectively.
30377844	4	33	theme	average	542:548	arg1	weights					560:566	The average molecular weights	538:566	The average molecular weights	538:566	The average molecular weights were determined as 28,342 Da and 41,143 Da, respectively.
30377844	4	33	theme	average	542:548	arg1	41,143 Da					601:609	41,143 Da	601:609	41,143 Da	601:609	The average molecular weights were determined as 28,342 Da and 41,143 Da, respectively.
30377844	10	34	theme	toll-like	1412:1420	arg1	TLR-4					1434:1438	TLR-4	1434:1438	TLR-4	1434:1438	In addition, both DLP-1 (5 μg/mL) and DLP-2 (50 μg/mL) significantly suppressed toll-like receptor-4 (TLR-4), myeloid differentiation factor (MyD88) and tumour necrosis factor receptor-associated factor-6 (TRAF-6) mRNA and protein expression in LPS-stimulated THP-1 cells.
30377844	10	34	theme	toll-like	1412:1420	arg1	receptor-4					1422:1431	toll-like receptor-4	1412:1431	toll-like receptor-4 (TLR-4)	1412:1439	In addition, both DLP-1 (5 μg/mL) and DLP-2 (50 μg/mL) significantly suppressed toll-like receptor-4 (TLR-4), myeloid differentiation factor (MyD88) and tumour necrosis factor receptor-associated factor-6 (TRAF-6) mRNA and protein expression in LPS-stimulated THP-1 cells.
30377844	2	35	theme	Dendrobium	301:310	arg1	officinale					312:321	Dendrobium officinale leaves	301:328	Dendrobium officinale leaves	301:328	However, little was known about the polysaccharides from Dendrobium officinale leaves.
30377844	0	36	theme	polysaccharides	13:27	arg1	Isolation					0:8	Isolation	0:8	Isolation of polysaccharides from Dendrobium officinale	0:54	Isolation of polysaccharides from Dendrobium officinale leaves and anti-inflammatory activity in LPS-stimulated THP-1 cells.
30377844	10	37	from	expression	1563:1572	arg1	cells					1598:1602	LPS-stimulated THP-1 cells	1577:1602	LPS-stimulated THP-1 cells	1577:1602	In addition, both DLP-1 (5 μg/mL) and DLP-2 (50 μg/mL) significantly suppressed toll-like receptor-4 (TLR-4), myeloid differentiation factor (MyD88) and tumour necrosis factor receptor-associated factor-6 (TRAF-6) mRNA and protein expression in LPS-stimulated THP-1 cells.
30377844	3	38	from	polysaccharides	344:358	arg1	the					363:365	the	363:365	the	363:365	Two kinds of polysaccharides in the leaves, DLP-1 and DLP-2, were obtained by hot water extraction, alcohol sedimentation and chromatographic separation (DEAE-52 cellulose column and Sephadex G-100 column).
30377844	1	39	theme	Dendrobium	125:134	arg1	stem					147:150	Dendrobium officinale stem	125:150	Dendrobium officinale stem	125:150	Dendrobium officinale stem is rich in polysaccharides, which play a great role in the medicinal effects of this plant.
30377844	3	40	theme	chromatographic	457:471	arg1	separation					473:482	chromatographic separation	457:482	chromatographic separation (DEAE-52 cellulose column and Sephadex G-100 column)	457:535	Two kinds of polysaccharides in the leaves, DLP-1 and DLP-2, were obtained by hot water extraction, alcohol sedimentation and chromatographic separation (DEAE-52 cellulose column and Sephadex G-100 column).
30377844	3	41	theme	alcohol	431:437	arg1	sedimentation					439:451	alcohol sedimentation	431:451	alcohol sedimentation	431:451	Two kinds of polysaccharides in the leaves, DLP-1 and DLP-2, were obtained by hot water extraction, alcohol sedimentation and chromatographic separation (DEAE-52 cellulose column and Sephadex G-100 column).
30377844	10	42	theme	mRNA	1546:1549	arg1	expression					1563:1572	tumour necrosis factor receptor-associated factor-6 (TRAF-6) mRNA and protein expression	1485:1572	tumour necrosis factor receptor-associated factor-6 (TRAF-6) mRNA and protein expression in LPS-stimulated THP-1 cells	1485:1602	In addition, both DLP-1 (5 μg/mL) and DLP-2 (50 μg/mL) significantly suppressed toll-like receptor-4 (TLR-4), myeloid differentiation factor (MyD88) and tumour necrosis factor receptor-associated factor-6 (TRAF-6) mRNA and protein expression in LPS-stimulated THP-1 cells.
30377844	1	43	theme	medicinal	211:219	arg1	effects					221:227	the medicinal effects	207:227	the medicinal effects of this plant	207:241	Dendrobium officinale stem is rich in polysaccharides, which play a great role in the medicinal effects of this plant.
30377844	0	44	theme	Dendrobium	34:43	arg1	officinale					45:54	Dendrobium officinale	34:54	Dendrobium officinale	34:54	Isolation of polysaccharides from Dendrobium officinale leaves and anti-inflammatory activity in LPS-stimulated THP-1 cells.
30377844	7	45	theme	D-xylose	976:983	arg1	3.23:1.02					951:959	3.23:1.02	951:959	3.23:1.02	951:959	DLP-2 was consisted of D-glucose and D-(+)-galactose with a molar ratio of 3.23:1.02, and traces of D-xylose, DL-arabinose.
30377844	7	45	theme	D-xylose	976:983	arg1	traces					966:971	traces	966:971	traces	966:971	DLP-2 was consisted of D-glucose and D-(+)-galactose with a molar ratio of 3.23:1.02, and traces of D-xylose, DL-arabinose.
30377844	0	46	from	activity	85:92	arg1	cells					118:122	LPS-stimulated THP-1 cells	97:122	LPS-stimulated THP-1 cells	97:122	Isolation of polysaccharides from Dendrobium officinale leaves and anti-inflammatory activity in LPS-stimulated THP-1 cells.
30377844	7	47	with	-galactose	918:927	arg1	ratio					942:946	a molar ratio	934:946	a molar ratio of 3.23:1.02, and traces of D-xylose, DL-arabinose	934:997	DLP-2 was consisted of D-glucose and D-(+)-galactose with a molar ratio of 3.23:1.02, and traces of D-xylose, DL-arabinose.
30377844	8	48	theme	inflammatory cell model	1021:1043	arg1	cells					1065:1069	inflammatory cell model by LPS acting THP-1 cells	1021:1069	inflammatory cell model by LPS acting THP-1 cells	1021:1069	Then, we established inflammatory cell model by LPS acting THP-1 cells to investigate the anti-inflammatory effects of DLP-1 and DLP-2.
30377844	8	49	theme	THP-1	1059:1063	arg1	cells					1065:1069	inflammatory cell model by LPS acting THP-1 cells	1021:1069	inflammatory cell model by LPS acting THP-1 cells	1021:1069	Then, we established inflammatory cell model by LPS acting THP-1 cells to investigate the anti-inflammatory effects of DLP-1 and DLP-2.
30377844	7	50	theme	3.23:1.02	951:959	arg1	ratio					942:946	a molar ratio	934:946	a molar ratio of 3.23:1.02, and traces of D-xylose, DL-arabinose	934:997	DLP-2 was consisted of D-glucose and D-(+)-galactose with a molar ratio of 3.23:1.02, and traces of D-xylose, DL-arabinose.
30377844	3	51	theme	DEAE-52	485:491	arg1	column					503:508	DEAE-52 cellulose column	485:508	DEAE-52 cellulose column	485:508	Two kinds of polysaccharides in the leaves, DLP-1 and DLP-2, were obtained by hot water extraction, alcohol sedimentation and chromatographic separation (DEAE-52 cellulose column and Sephadex G-100 column).
30377844	6	52	with	-rhamnose	774:782	arg1	ratio					797:801	a molar ratio	789:801	a molar ratio of 3.21:1.11:0.23	789:819	DLP-1 was composed of D-(+)-galactose, DL-arabinose, and L-(+)-rhamnose with a molar ratio of 3.21:1.11:0.23, and traces of D-xylose, D-glucose, and D-(+)-mannose.
30377844	7	53	theme	traces	966:971	arg1	ratio					942:946	a molar ratio	934:946	a molar ratio of 3.23:1.02, and traces of D-xylose, DL-arabinose	934:997	DLP-2 was consisted of D-glucose and D-(+)-galactose with a molar ratio of 3.23:1.02, and traces of D-xylose, DL-arabinose.
30377844	10	54	theme	LPS-stimulated	1577:1590	arg1	cells					1598:1602	LPS-stimulated THP-1 cells	1577:1602	LPS-stimulated THP-1 cells	1577:1602	In addition, both DLP-1 (5 μg/mL) and DLP-2 (50 μg/mL) significantly suppressed toll-like receptor-4 (TLR-4), myeloid differentiation factor (MyD88) and tumour necrosis factor receptor-associated factor-6 (TRAF-6) mRNA and protein expression in LPS-stimulated THP-1 cells.
30377844	10	55	from	receptor-4	1422:1431	arg1	cells					1598:1602	LPS-stimulated THP-1 cells	1577:1602	LPS-stimulated THP-1 cells	1577:1602	In addition, both DLP-1 (5 μg/mL) and DLP-2 (50 μg/mL) significantly suppressed toll-like receptor-4 (TLR-4), myeloid differentiation factor (MyD88) and tumour necrosis factor receptor-associated factor-6 (TRAF-6) mRNA and protein expression in LPS-stimulated THP-1 cells.
30377844	3	56	theme	cellulose	493:501	arg1	column					503:508	DEAE-52 cellulose column	485:508	DEAE-52 cellulose column	485:508	Two kinds of polysaccharides in the leaves, DLP-1 and DLP-2, were obtained by hot water extraction, alcohol sedimentation and chromatographic separation (DEAE-52 cellulose column and Sephadex G-100 column).
30377844	9	57	theme	reactive	1297:1304	arg1	species					1313:1319	reactive oxygen species	1297:1319	reactive oxygen species formation	1297:1329	The results indicated that DLP-1 (5 μg/mL) and DLP-2 (50 μg/mL) were effective in protecting THP-1 cells from LPS-stimulated cytotoxicity, as well as inhibiting reactive oxygen species formation.
30377844	3	58	dep	the	363:365	arg1	leaves					367:372	leaves	367:372	leaves	367:372	Two kinds of polysaccharides in the leaves, DLP-1 and DLP-2, were obtained by hot water extraction, alcohol sedimentation and chromatographic separation (DEAE-52 cellulose column and Sephadex G-100 column).
30377844	8	59	theme	acting	1052:1057	arg1	cells					1065:1069	inflammatory cell model by LPS acting THP-1 cells	1021:1069	inflammatory cell model by LPS acting THP-1 cells	1021:1069	Then, we established inflammatory cell model by LPS acting THP-1 cells to investigate the anti-inflammatory effects of DLP-1 and DLP-2.
30377844	7	60	theme	molar	936:940	arg1	ratio					942:946	a molar ratio	934:946	a molar ratio of 3.23:1.02, and traces of D-xylose, DL-arabinose	934:997	DLP-2 was consisted of D-glucose and D-(+)-galactose with a molar ratio of 3.23:1.02, and traces of D-xylose, DL-arabinose.
30377844	9	61	theme	oxygen	1306:1311	arg1	species					1313:1319	reactive oxygen species	1297:1319	reactive oxygen species formation	1297:1329	The results indicated that DLP-1 (5 μg/mL) and DLP-2 (50 μg/mL) were effective in protecting THP-1 cells from LPS-stimulated cytotoxicity, as well as inhibiting reactive oxygen species formation.
30377844	8	62	theme	by LPS	1045:1050	arg1	cells					1065:1069	inflammatory cell model by LPS acting THP-1 cells	1021:1069	inflammatory cell model by LPS acting THP-1 cells	1021:1069	Then, we established inflammatory cell model by LPS acting THP-1 cells to investigate the anti-inflammatory effects of DLP-1 and DLP-2.
30377844	3	63	from	kinds	335:339	arg1	the					363:365	the	363:365	the	363:365	Two kinds of polysaccharides in the leaves, DLP-1 and DLP-2, were obtained by hot water extraction, alcohol sedimentation and chromatographic separation (DEAE-52 cellulose column and Sephadex G-100 column).
30377844	6	64	theme	DL-arabinose	751:762	arg1	D-					734:735	D-	734:735	D-	734:735	DLP-1 was composed of D-(+)-galactose, DL-arabinose, and L-(+)-rhamnose with a molar ratio of 3.21:1.11:0.23, and traces of D-xylose, D-glucose, and D-(+)-mannose.
30377844	2	65	dep	officinale	312:321	arg1	leaves					323:328	leaves	323:328	leaves	323:328	However, little was known about the polysaccharides from Dendrobium officinale leaves.
30377844	9	66	theme	species	1313:1319	arg1	formation					1321:1329	reactive oxygen species formation	1297:1329	reactive oxygen species formation	1297:1329	The results indicated that DLP-1 (5 μg/mL) and DLP-2 (50 μg/mL) were effective in protecting THP-1 cells from LPS-stimulated cytotoxicity, as well as inhibiting reactive oxygen species formation.
30377844	6	67	theme	3.21:1.11:0.23	806:819	arg1	ratio					797:801	a molar ratio	789:801	a molar ratio of 3.21:1.11:0.23	789:819	DLP-1 was composed of D-(+)-galactose, DL-arabinose, and L-(+)-rhamnose with a molar ratio of 3.21:1.11:0.23, and traces of D-xylose, D-glucose, and D-(+)-mannose.
30377844	3	68	theme	Sephadex	514:521	arg1	column					529:534	Sephadex G-100 column	514:534	Sephadex G-100 column	514:534	Two kinds of polysaccharides in the leaves, DLP-1 and DLP-2, were obtained by hot water extraction, alcohol sedimentation and chromatographic separation (DEAE-52 cellulose column and Sephadex G-100 column).
30377844	1	69	theme	plant	237:241	arg1	effects					221:227	the medicinal effects	207:227	the medicinal effects of this plant	207:241	Dendrobium officinale stem is rich in polysaccharides, which play a great role in the medicinal effects of this plant.
30377844	0	70	theme	anti-inflammatory	67:83	arg1	activity					85:92	anti-inflammatory activity	67:92	anti-inflammatory activity	67:92	Isolation of polysaccharides from Dendrobium officinale leaves and anti-inflammatory activity in LPS-stimulated THP-1 cells.
30377844	8	71	theme	DLP-2	1129:1133	arg1	effects					1108:1114	the anti-inflammatory effects	1086:1114	the anti-inflammatory effects of DLP-1 and DLP-2	1086:1133	Then, we established inflammatory cell model by LPS acting THP-1 cells to investigate the anti-inflammatory effects of DLP-1 and DLP-2.
30377844	6	72	theme	-galactose	739:748	arg1	D-					734:735	D-	734:735	D-	734:735	DLP-1 was composed of D-(+)-galactose, DL-arabinose, and L-(+)-rhamnose with a molar ratio of 3.21:1.11:0.23, and traces of D-xylose, D-glucose, and D-(+)-mannose.
30377844	3	73	theme	G-100	523:527	arg1	column					529:534	Sephadex G-100 column	514:534	Sephadex G-100 column	514:534	Two kinds of polysaccharides in the leaves, DLP-1 and DLP-2, were obtained by hot water extraction, alcohol sedimentation and chromatographic separation (DEAE-52 cellulose column and Sephadex G-100 column).
30839250	5	0	dep	anteiso-C15 	468:479	arg1	 0					481:482	 0	481:482	 0	481:482	The major fatty acids of strain GY 10110T were anteiso-C15 : 0, iso-C15 : 0 and iso-C16 : 0.
30839250	5	0	dep	anteiso-C15 	468:479	arg1	anteiso-C15 					468:479	anteiso-C15 	468:479	anteiso-C15 	468:479	The major fatty acids of strain GY 10110T were anteiso-C15 : 0, iso-C15 : 0 and iso-C16 : 0.
30839250	5	0	dep	anteiso-C15 	468:479	arg1	acids					437:441	The major fatty acids	421:441	The major fatty acids of strain GY 10110T	421:461	The major fatty acids of strain GY 10110T were anteiso-C15 : 0, iso-C15 : 0 and iso-C16 : 0.
30839250	9	1	theme	strain	872:877	arg1	10110T					882:887	strain GY 10110T	872:887	strain GY 10110T	872:887	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain GY 10110T was closely related to Falsibacillus pallidus CCTCC AB 207188T (98.0 % sequence similarity) and Bacillus oceanisediminis CGMCC 1.10115T (96.9 %), respectively.
30839250	2	2	theme	oxidase-positive	102:117	arg1	10110T					163:168	designated GY 10110T	149:168	designated GY 10110T	149:168	A novel Gram-stain-positive, catalase- and oxidase-positive, endospore-forming bacterium, designated GY 10110T, was isolated from mangrove soil collected from Qinzhou, Guangxi province, China.
30839250	2	2	theme	oxidase-positive	102:117	arg1	bacterium					138:146	A novel Gram-stain-positive, catalase- and oxidase-positive, endospore-forming bacterium	59:146	bacterium	138:146	A novel Gram-stain-positive, catalase- and oxidase-positive, endospore-forming bacterium, designated GY 10110T, was isolated from mangrove soil collected from Qinzhou, Guangxi province, China.
30839250	10	3	theme	42.3 mol	1124:1131	arg1	G+C content					1053:1063	The G+C content	1049:1063	The G+C content of strain GY 10110T based on the whole genome sequence	1049:1118	The G+C content of strain GY 10110T based on the whole genome sequence was 42.3 mol%.
30839250	10	3	theme	42.3 mol	1124:1131	arg1	%					1132:1132	42.3 mol%	1124:1132	42.3 mol%	1124:1132	The G+C content of strain GY 10110T based on the whole genome sequence was 42.3 mol%.
30839250	11	4	theme	CCTCC	1299:1303	arg1	207188T					1308:1314	Falsibacillus pallidus CCTCC AB 207188T	1276:1314	Falsibacillus pallidus CCTCC AB 207188T	1276:1314	The novel strain showed an average nucleotide identity (ANI) value of 77.8 % and a digital DNA-DNA hybridization (dDDH) value of 15.6 % with Falsibacillus pallidus CCTCC AB 207188T based on draft genome sequences, followed by Bacillus oceanisediminis CGMCC 1.10115T with ANI and dDDH values of 75.2 and 12.8 %, respectively.
30839250	2	5	theme	GY	160:161	arg1	10110T					163:168	designated GY 10110T	149:168	designated GY 10110T	149:168	A novel Gram-stain-positive, catalase- and oxidase-positive, endospore-forming bacterium, designated GY 10110T, was isolated from mangrove soil collected from Qinzhou, Guangxi province, China.
30839250	2	5	theme	GY	160:161	arg1	bacterium					138:146	A novel Gram-stain-positive, catalase- and oxidase-positive, endospore-forming bacterium	59:146	bacterium	138:146	A novel Gram-stain-positive, catalase- and oxidase-positive, endospore-forming bacterium, designated GY 10110T, was isolated from mangrove soil collected from Qinzhou, Guangxi province, China.
30839250	7	6	theme	meso-diaminopimelic	590:608	arg1	acid					610:613	meso-diaminopimelic acid	590:613	meso-diaminopimelic acid	590:613	The cell-wall peptidoglycan contained meso-diaminopimelic acid.
30839250	12	7	theme	genus	1634:1638	arg1	Falsibacillus					1640:1652	the genus Falsibacillus	1630:1652	the genus Falsibacillus	1630:1652	The results of the polyphasic taxonomic study, including phenotypic, chemotaxonomic and phylogenetic analysis, showed that strain GY 10110T represents a novel species of the genus Falsibacillus, for which the name Falsibacillus albus sp.
30839250	11	8	theme	Falsibacillus	1276:1288	arg1	207188T					1308:1314	Falsibacillus pallidus CCTCC AB 207188T	1276:1314	Falsibacillus pallidus CCTCC AB 207188T	1276:1314	The novel strain showed an average nucleotide identity (ANI) value of 77.8 % and a digital DNA-DNA hybridization (dDDH) value of 15.6 % with Falsibacillus pallidus CCTCC AB 207188T based on draft genome sequences, followed by Bacillus oceanisediminis CGMCC 1.10115T with ANI and dDDH values of 75.2 and 12.8 %, respectively.
30839250	11	9	theme	DNA-DNA	1226:1232	arg1	dDDH					1249:1252	dDDH	1249:1252	dDDH	1249:1252	The novel strain showed an average nucleotide identity (ANI) value of 77.8 % and a digital DNA-DNA hybridization (dDDH) value of 15.6 % with Falsibacillus pallidus CCTCC AB 207188T based on draft genome sequences, followed by Bacillus oceanisediminis CGMCC 1.10115T with ANI and dDDH values of 75.2 and 12.8 %, respectively.
30839250	11	9	theme	DNA-DNA	1226:1232	arg1	hybridization					1234:1246	a digital DNA-DNA hybridization	1216:1246	a digital DNA-DNA hybridization (dDDH) value of 15.6 % with Falsibacillus pallidus CCTCC AB 207188T	1216:1314	The novel strain showed an average nucleotide identity (ANI) value of 77.8 % and a digital DNA-DNA hybridization (dDDH) value of 15.6 % with Falsibacillus pallidus CCTCC AB 207188T based on draft genome sequences, followed by Bacillus oceanisediminis CGMCC 1.10115T with ANI and dDDH values of 75.2 and 12.8 %, respectively.
30839250	14	10	theme	=CGMCC	1746:1751	arg1	10110T					1738:1743	GY 10110T	1735:1743	GY 10110T (=CGMCC 1.13648T=NBRC 113502T)	1735:1774	The type strain is GY 10110T (=CGMCC 1.13648T=NBRC 113502T).
30839250	14	10	theme	=CGMCC	1746:1751	arg1	113502T					1767:1773	=CGMCC 1.13648T=NBRC 113502T	1746:1773	=CGMCC 1.13648T=NBRC 113502T	1746:1773	The type strain is GY 10110T (=CGMCC 1.13648T=NBRC 113502T).
30839250	12	11	theme	novel	1613:1617	arg1	species					1619:1625	a novel species	1611:1625	a novel species	1611:1625	The results of the polyphasic taxonomic study, including phenotypic, chemotaxonomic and phylogenetic analysis, showed that strain GY 10110T represents a novel species of the genus Falsibacillus, for which the name Falsibacillus albus sp.
30839250	7	12	theme	cell-wall	556:564	arg1	peptidoglycan					566:578	The cell-wall peptidoglycan	552:578	The cell-wall peptidoglycan	552:578	The cell-wall peptidoglycan contained meso-diaminopimelic acid.
30839250	9	13	theme	AB	941:942	arg1	similarity					969:978	98.0 % sequence similarity	953:978	98.0 % sequence similarity	953:978	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain GY 10110T was closely related to Falsibacillus pallidus CCTCC AB 207188T (98.0 % sequence similarity) and Bacillus oceanisediminis CGMCC 1.10115T (96.9 %), respectively.
30839250	9	13	theme	AB	941:942	arg1	207188T					944:950	Falsibacillus pallidus CCTCC AB 207188T	912:950	Falsibacillus pallidus CCTCC AB 207188T (98.0 % sequence similarity)	912:979	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain GY 10110T was closely related to Falsibacillus pallidus CCTCC AB 207188T (98.0 % sequence similarity) and Bacillus oceanisediminis CGMCC 1.10115T (96.9 %), respectively.
30839250	2	14	theme	catalase-	88:96	arg1	10110T					163:168	designated GY 10110T	149:168	designated GY 10110T	149:168	A novel Gram-stain-positive, catalase- and oxidase-positive, endospore-forming bacterium, designated GY 10110T, was isolated from mangrove soil collected from Qinzhou, Guangxi province, China.
30839250	2	14	theme	catalase-	88:96	arg1	bacterium					138:146	A novel Gram-stain-positive, catalase- and oxidase-positive, endospore-forming bacterium	59:146	bacterium	138:146	A novel Gram-stain-positive, catalase- and oxidase-positive, endospore-forming bacterium, designated GY 10110T, was isolated from mangrove soil collected from Qinzhou, Guangxi province, China.
30839250	5	15	theme	fatty	431:435	arg1	acids					437:441	The major fatty acids	421:441	The major fatty acids of strain GY 10110T	421:461	The major fatty acids of strain GY 10110T were anteiso-C15 : 0, iso-C15 : 0 and iso-C16 : 0.
30839250	5	15	theme	fatty	431:435	arg1	anteiso-C15 					468:479	anteiso-C15 	468:479	anteiso-C15 	468:479	The major fatty acids of strain GY 10110T were anteiso-C15 : 0, iso-C15 : 0 and iso-C16 : 0.
30839250	5	15	theme	fatty	431:435	arg1	 0					481:482	 0	481:482	 0	481:482	The major fatty acids of strain GY 10110T were anteiso-C15 : 0, iso-C15 : 0 and iso-C16 : 0.
30839250	2	16	theme	novel	61:65	arg1	10110T					163:168	designated GY 10110T	149:168	designated GY 10110T	149:168	A novel Gram-stain-positive, catalase- and oxidase-positive, endospore-forming bacterium, designated GY 10110T, was isolated from mangrove soil collected from Qinzhou, Guangxi province, China.
30839250	2	16	theme	novel	61:65	arg1	bacterium					138:146	A novel Gram-stain-positive, catalase- and oxidase-positive, endospore-forming bacterium	59:146	bacterium	138:146	A novel Gram-stain-positive, catalase- and oxidase-positive, endospore-forming bacterium, designated GY 10110T, was isolated from mangrove soil collected from Qinzhou, Guangxi province, China.
30839250	3	17	theme	peritrichous	284:295	arg1	flagella					297:304	peritrichous flagella	284:304	peritrichous flagella	284:304	Cells were aerobic, motile with peritrichous flagella and rod-shaped.
30839250	2	18	theme	Gram-stain-positive	67:85	arg1	10110T					163:168	designated GY 10110T	149:168	designated GY 10110T	149:168	A novel Gram-stain-positive, catalase- and oxidase-positive, endospore-forming bacterium, designated GY 10110T, was isolated from mangrove soil collected from Qinzhou, Guangxi province, China.
30839250	2	18	theme	Gram-stain-positive	67:85	arg1	bacterium					138:146	A novel Gram-stain-positive, catalase- and oxidase-positive, endospore-forming bacterium	59:146	bacterium	138:146	A novel Gram-stain-positive, catalase- and oxidase-positive, endospore-forming bacterium, designated GY 10110T, was isolated from mangrove soil collected from Qinzhou, Guangxi province, China.
30839250	14	19	theme	type	1720:1723	arg1	10110T					1738:1743	GY 10110T	1735:1743	GY 10110T (=CGMCC 1.13648T=NBRC 113502T)	1735:1774	The type strain is GY 10110T (=CGMCC 1.13648T=NBRC 113502T).
30839250	14	19	theme	type	1720:1723	arg1	strain					1725:1730	The type strain	1716:1730	The type strain	1716:1730	The type strain is GY 10110T (=CGMCC 1.13648T=NBRC 113502T).
30839250	12	20	theme	strain	1583:1588	arg1	10110T					1593:1598	strain GY 10110T	1583:1598	strain GY 10110T	1583:1598	The results of the polyphasic taxonomic study, including phenotypic, chemotaxonomic and phylogenetic analysis, showed that strain GY 10110T represents a novel species of the genus Falsibacillus, for which the name Falsibacillus albus sp.
30839250	4	21	theme	%	375:375	arg1	NaCl					382:385	0-3 %(w/v) NaCl	371:385	0-3 %(w/v) NaCl (1 %)	371:391	The strain grew at 15-37 °C (optimum, 28 °C), at 0-3 %(w/v) NaCl (1 %) and at pH 6.0-9.0 (pH 7.0).
30839250	4	21	theme	%	375:375	arg1	%					390:390	1 %	388:390	1 %	388:390	The strain grew at 15-37 °C (optimum, 28 °C), at 0-3 %(w/v) NaCl (1 %) and at pH 6.0-9.0 (pH 7.0).
30839250	9	22	theme	%	958:958	arg1	similarity					969:978	98.0 % sequence similarity	953:978	98.0 % sequence similarity	953:978	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain GY 10110T was closely related to Falsibacillus pallidus CCTCC AB 207188T (98.0 % sequence similarity) and Bacillus oceanisediminis CGMCC 1.10115T (96.9 %), respectively.
30839250	9	22	theme	%	958:958	arg1	207188T					944:950	Falsibacillus pallidus CCTCC AB 207188T	912:950	Falsibacillus pallidus CCTCC AB 207188T (98.0 % sequence similarity)	912:979	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain GY 10110T was closely related to Falsibacillus pallidus CCTCC AB 207188T (98.0 % sequence similarity) and Bacillus oceanisediminis CGMCC 1.10115T (96.9 %), respectively.
30839250	4	23	theme	w/v	377:379	arg1	NaCl					382:385	0-3 %(w/v) NaCl	371:385	0-3 %(w/v) NaCl (1 %)	371:391	The strain grew at 15-37 °C (optimum, 28 °C), at 0-3 %(w/v) NaCl (1 %) and at pH 6.0-9.0 (pH 7.0).
30839250	4	23	theme	w/v	377:379	arg1	%					390:390	1 %	388:390	1 %	388:390	The strain grew at 15-37 °C (optimum, 28 °C), at 0-3 %(w/v) NaCl (1 %) and at pH 6.0-9.0 (pH 7.0).
30839250	11	24	with	1.10115T	1392:1399	arg1	values					1419:1424	ANI and dDDH values	1406:1424	values	1419:1424	The novel strain showed an average nucleotide identity (ANI) value of 77.8 % and a digital DNA-DNA hybridization (dDDH) value of 15.6 % with Falsibacillus pallidus CCTCC AB 207188T based on draft genome sequences, followed by Bacillus oceanisediminis CGMCC 1.10115T with ANI and dDDH values of 75.2 and 12.8 %, respectively.
30839250	11	25	theme	ANI	1191:1193	arg1	value					1196:1200	an average nucleotide identity (ANI) value	1159:1200	an average nucleotide identity (ANI) value of 77.8 %	1159:1210	The novel strain showed an average nucleotide identity (ANI) value of 77.8 % and a digital DNA-DNA hybridization (dDDH) value of 15.6 % with Falsibacillus pallidus CCTCC AB 207188T based on draft genome sequences, followed by Bacillus oceanisediminis CGMCC 1.10115T with ANI and dDDH values of 75.2 and 12.8 %, respectively.
30839250	11	26	theme	ANI	1406:1408	arg1	values					1419:1424	ANI and dDDH values	1406:1424	values	1419:1424	The novel strain showed an average nucleotide identity (ANI) value of 77.8 % and a digital DNA-DNA hybridization (dDDH) value of 15.6 % with Falsibacillus pallidus CCTCC AB 207188T based on draft genome sequences, followed by Bacillus oceanisediminis CGMCC 1.10115T with ANI and dDDH values of 75.2 and 12.8 %, respectively.
30839250	11	27	theme	identity	1181:1188	arg1	value					1196:1200	an average nucleotide identity (ANI) value	1159:1200	an average nucleotide identity (ANI) value of 77.8 %	1159:1210	The novel strain showed an average nucleotide identity (ANI) value of 77.8 % and a digital DNA-DNA hybridization (dDDH) value of 15.6 % with Falsibacillus pallidus CCTCC AB 207188T based on draft genome sequences, followed by Bacillus oceanisediminis CGMCC 1.10115T with ANI and dDDH values of 75.2 and 12.8 %, respectively.
30839250	5	28	theme	GY	453:454	arg1	10110T					456:461	strain GY 10110T	446:461	strain GY 10110T	446:461	The major fatty acids of strain GY 10110T were anteiso-C15 : 0, iso-C15 : 0 and iso-C16 : 0.
30839250	0	29	theme	Falsibacillus	0:12	arg1	albus					14:18	Falsibacillus albus	0:18	Falsibacillus albus	0:18	Falsibacillus albus sp.
30839250	12	30	theme	study	1500:1504	arg1	chemotaxonomic					1529:1542	chemotaxonomic	1529:1542	chemotaxonomic	1529:1542	The results of the polyphasic taxonomic study, including phenotypic, chemotaxonomic and phylogenetic analysis, showed that strain GY 10110T represents a novel species of the genus Falsibacillus, for which the name Falsibacillus albus sp.
30839250	12	30	theme	study	1500:1504	arg1	phenotypic					1517:1526	phenotypic	1517:1526	phenotypic	1517:1526	The results of the polyphasic taxonomic study, including phenotypic, chemotaxonomic and phylogenetic analysis, showed that strain GY 10110T represents a novel species of the genus Falsibacillus, for which the name Falsibacillus albus sp.
30839250	12	30	theme	study	1500:1504	arg1	results					1464:1470	The results	1460:1470	The results	1460:1470	The results of the polyphasic taxonomic study, including phenotypic, chemotaxonomic and phylogenetic analysis, showed that strain GY 10110T represents a novel species of the genus Falsibacillus, for which the name Falsibacillus albus sp.
30839250	12	30	theme	study	1500:1504	arg1	analysis					1561:1568	phylogenetic analysis	1548:1568	phylogenetic analysis	1548:1568	The results of the polyphasic taxonomic study, including phenotypic, chemotaxonomic and phylogenetic analysis, showed that strain GY 10110T represents a novel species of the genus Falsibacillus, for which the name Falsibacillus albus sp.
30839250	11	31	theme	novel	1139:1143	arg1	strain					1145:1150	The novel strain	1135:1150	The novel strain	1135:1150	The novel strain showed an average nucleotide identity (ANI) value of 77.8 % and a digital DNA-DNA hybridization (dDDH) value of 15.6 % with Falsibacillus pallidus CCTCC AB 207188T based on draft genome sequences, followed by Bacillus oceanisediminis CGMCC 1.10115T with ANI and dDDH values of 75.2 and 12.8 %, respectively.
30839250	6	32	theme	predominant	518:528	arg1	MK-7					546:549	MK-7	546:549	MK-7	546:549	The predominant menaquinone was MK-7.
30839250	6	32	theme	predominant	518:528	arg1	menaquinone					530:540	The predominant menaquinone	514:540	The predominant menaquinone	514:540	The predominant menaquinone was MK-7.
30839250	2	33	attach	isolated	175:182	arg2	10110T					163:168	designated GY 10110T	149:168	designated GY 10110T	149:168	A novel Gram-stain-positive, catalase- and oxidase-positive, endospore-forming bacterium, designated GY 10110T, was isolated from mangrove soil collected from Qinzhou, Guangxi province, China.
30839250	2	33	attach	isolated	175:182	arg1	soil					198:201	mangrove soil	189:201	mangrove soil collected from Qinzhou, Guangxi province, China	189:249	A novel Gram-stain-positive, catalase- and oxidase-positive, endospore-forming bacterium, designated GY 10110T, was isolated from mangrove soil collected from Qinzhou, Guangxi province, China.
30839250	2	33	attach	isolated	175:182	arg2	bacterium					138:146	A novel Gram-stain-positive, catalase- and oxidase-positive, endospore-forming bacterium	59:146	bacterium	138:146	A novel Gram-stain-positive, catalase- and oxidase-positive, endospore-forming bacterium, designated GY 10110T, was isolated from mangrove soil collected from Qinzhou, Guangxi province, China.
30839250	11	34	theme	genome	1331:1336	arg1	sequences					1338:1346	draft genome sequences	1325:1346	draft genome sequences	1325:1346	The novel strain showed an average nucleotide identity (ANI) value of 77.8 % and a digital DNA-DNA hybridization (dDDH) value of 15.6 % with Falsibacillus pallidus CCTCC AB 207188T based on draft genome sequences, followed by Bacillus oceanisediminis CGMCC 1.10115T with ANI and dDDH values of 75.2 and 12.8 %, respectively.
30839250	12	35	theme	Falsibacillus	1674:1686	arg1	sp					1694:1695	Falsibacillus albus sp	1674:1695	the name Falsibacillus albus sp	1665:1695	The results of the polyphasic taxonomic study, including phenotypic, chemotaxonomic and phylogenetic analysis, showed that strain GY 10110T represents a novel species of the genus Falsibacillus, for which the name Falsibacillus albus sp.
30839250	11	36	theme	average	1162:1168	arg1	value					1196:1200	an average nucleotide identity (ANI) value	1159:1200	an average nucleotide identity (ANI) value of 77.8 %	1159:1210	The novel strain showed an average nucleotide identity (ANI) value of 77.8 % and a digital DNA-DNA hybridization (dDDH) value of 15.6 % with Falsibacillus pallidus CCTCC AB 207188T based on draft genome sequences, followed by Bacillus oceanisediminis CGMCC 1.10115T with ANI and dDDH values of 75.2 and 12.8 %, respectively.
30839250	10	37	theme	GY	1075:1076	arg1	10110T					1078:1083	strain GY 10110T	1068:1083	strain GY 10110T	1068:1083	The G+C content of strain GY 10110T based on the whole genome sequence was 42.3 mol%.
30839250	9	38	theme	rRNA	840:843	arg1	sequences					850:858	16S rRNA gene sequences	836:858	16S rRNA gene sequences	836:858	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain GY 10110T was closely related to Falsibacillus pallidus CCTCC AB 207188T (98.0 % sequence similarity) and Bacillus oceanisediminis CGMCC 1.10115T (96.9 %), respectively.
30839250	10	39	theme	genome	1104:1109	arg1	sequence					1111:1118	the whole genome sequence	1094:1118	the whole genome sequence	1094:1118	The G+C content of strain GY 10110T based on the whole genome sequence was 42.3 mol%.
30839250	0	40	dep	sp	20:21	arg1	albus					14:18	Falsibacillus albus	0:18	Falsibacillus albus	0:18	Falsibacillus albus sp.
30839250	8	41	theme	unidentified	735:746	arg1	aminophospholipids					748:765	two unidentified aminophospholipids	731:765	two unidentified aminophospholipids	731:765	The polar lipid profile comprised diphosphatidylglycerol, phosphatidylethanolamine, phosphoglycolipid, glycolipid, two unidentified aminophospholipids and three unidentified phospholipids.
30839250	10	42	theme	10110T	1078:1083	arg1	G+C content					1053:1063	The G+C content	1049:1063	The G+C content of strain GY 10110T based on the whole genome sequence	1049:1118	The G+C content of strain GY 10110T based on the whole genome sequence was 42.3 mol%.
30839250	10	42	theme	10110T	1078:1083	arg1	%					1132:1132	42.3 mol%	1124:1132	42.3 mol%	1124:1132	The G+C content of strain GY 10110T based on the whole genome sequence was 42.3 mol%.
30839250	9	43	theme	GY	879:880	arg1	10110T					882:887	strain GY 10110T	872:887	strain GY 10110T	872:887	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain GY 10110T was closely related to Falsibacillus pallidus CCTCC AB 207188T (98.0 % sequence similarity) and Bacillus oceanisediminis CGMCC 1.10115T (96.9 %), respectively.
30839250	12	44	theme	Falsibacillus	1640:1652	arg1	species					1619:1625	a novel species	1611:1625	a novel species	1611:1625	The results of the polyphasic taxonomic study, including phenotypic, chemotaxonomic and phylogenetic analysis, showed that strain GY 10110T represents a novel species of the genus Falsibacillus, for which the name Falsibacillus albus sp.
30839250	2	45	theme	designated	149:158	arg1	10110T					163:168	designated GY 10110T	149:168	designated GY 10110T	149:168	A novel Gram-stain-positive, catalase- and oxidase-positive, endospore-forming bacterium, designated GY 10110T, was isolated from mangrove soil collected from Qinzhou, Guangxi province, China.
30839250	2	45	theme	designated	149:158	arg1	bacterium					138:146	A novel Gram-stain-positive, catalase- and oxidase-positive, endospore-forming bacterium	59:146	bacterium	138:146	A novel Gram-stain-positive, catalase- and oxidase-positive, endospore-forming bacterium, designated GY 10110T, was isolated from mangrove soil collected from Qinzhou, Guangxi province, China.
30839250	11	46	theme	dDDH	1414:1417	arg1	values					1419:1424	ANI and dDDH values	1406:1424	values	1419:1424	The novel strain showed an average nucleotide identity (ANI) value of 77.8 % and a digital DNA-DNA hybridization (dDDH) value of 15.6 % with Falsibacillus pallidus CCTCC AB 207188T based on draft genome sequences, followed by Bacillus oceanisediminis CGMCC 1.10115T with ANI and dDDH values of 75.2 and 12.8 %, respectively.
30839250	9	47	dep	Bacillus	985:992	arg1	oceanisediminis					994:1008	oceanisediminis	994:1008	oceanisediminis	994:1008	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain GY 10110T was closely related to Falsibacillus pallidus CCTCC AB 207188T (98.0 % sequence similarity) and Bacillus oceanisediminis CGMCC 1.10115T (96.9 %), respectively.
30839250	11	48	theme	digital	1218:1224	arg1	dDDH					1249:1252	dDDH	1249:1252	dDDH	1249:1252	The novel strain showed an average nucleotide identity (ANI) value of 77.8 % and a digital DNA-DNA hybridization (dDDH) value of 15.6 % with Falsibacillus pallidus CCTCC AB 207188T based on draft genome sequences, followed by Bacillus oceanisediminis CGMCC 1.10115T with ANI and dDDH values of 75.2 and 12.8 %, respectively.
30839250	11	48	theme	digital	1218:1224	arg1	hybridization					1234:1246	a digital DNA-DNA hybridization	1216:1246	a digital DNA-DNA hybridization (dDDH) value of 15.6 % with Falsibacillus pallidus CCTCC AB 207188T	1216:1314	The novel strain showed an average nucleotide identity (ANI) value of 77.8 % and a digital DNA-DNA hybridization (dDDH) value of 15.6 % with Falsibacillus pallidus CCTCC AB 207188T based on draft genome sequences, followed by Bacillus oceanisediminis CGMCC 1.10115T with ANI and dDDH values of 75.2 and 12.8 %, respectively.
30839250	11	49	theme	%	1443:1443	arg1	values					1419:1424	ANI and dDDH values	1406:1424	values	1419:1424	The novel strain showed an average nucleotide identity (ANI) value of 77.8 % and a digital DNA-DNA hybridization (dDDH) value of 15.6 % with Falsibacillus pallidus CCTCC AB 207188T based on draft genome sequences, followed by Bacillus oceanisediminis CGMCC 1.10115T with ANI and dDDH values of 75.2 and 12.8 %, respectively.
30839250	9	50	theme	16S	836:838	arg1	sequences					850:858	16S rRNA gene sequences	836:858	16S rRNA gene sequences	836:858	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain GY 10110T was closely related to Falsibacillus pallidus CCTCC AB 207188T (98.0 % sequence similarity) and Bacillus oceanisediminis CGMCC 1.10115T (96.9 %), respectively.
30839250	14	51	theme	1.13648T=NBRC	1753:1765	arg1	10110T					1738:1743	GY 10110T	1735:1743	GY 10110T (=CGMCC 1.13648T=NBRC 113502T)	1735:1774	The type strain is GY 10110T (=CGMCC 1.13648T=NBRC 113502T).
30839250	14	51	theme	1.13648T=NBRC	1753:1765	arg1	113502T					1767:1773	=CGMCC 1.13648T=NBRC 113502T	1746:1773	=CGMCC 1.13648T=NBRC 113502T	1746:1773	The type strain is GY 10110T (=CGMCC 1.13648T=NBRC 113502T).
30839250	9	52	theme	Falsibacillus	912:924	arg1	similarity					969:978	98.0 % sequence similarity	953:978	98.0 % sequence similarity	953:978	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain GY 10110T was closely related to Falsibacillus pallidus CCTCC AB 207188T (98.0 % sequence similarity) and Bacillus oceanisediminis CGMCC 1.10115T (96.9 %), respectively.
30839250	9	52	theme	Falsibacillus	912:924	arg1	207188T					944:950	Falsibacillus pallidus CCTCC AB 207188T	912:950	Falsibacillus pallidus CCTCC AB 207188T (98.0 % sequence similarity)	912:979	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain GY 10110T was closely related to Falsibacillus pallidus CCTCC AB 207188T (98.0 % sequence similarity) and Bacillus oceanisediminis CGMCC 1.10115T (96.9 %), respectively.
30839250	12	53	theme	polyphasic	1479:1488	arg1	study					1500:1504	the polyphasic taxonomic study	1475:1504	the polyphasic taxonomic study	1475:1504	The results of the polyphasic taxonomic study, including phenotypic, chemotaxonomic and phylogenetic analysis, showed that strain GY 10110T represents a novel species of the genus Falsibacillus, for which the name Falsibacillus albus sp.
30839250	9	54	theme	CCTCC	935:939	arg1	similarity					969:978	98.0 % sequence similarity	953:978	98.0 % sequence similarity	953:978	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain GY 10110T was closely related to Falsibacillus pallidus CCTCC AB 207188T (98.0 % sequence similarity) and Bacillus oceanisediminis CGMCC 1.10115T (96.9 %), respectively.
30839250	9	54	theme	CCTCC	935:939	arg1	207188T					944:950	Falsibacillus pallidus CCTCC AB 207188T	912:950	Falsibacillus pallidus CCTCC AB 207188T (98.0 % sequence similarity)	912:979	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain GY 10110T was closely related to Falsibacillus pallidus CCTCC AB 207188T (98.0 % sequence similarity) and Bacillus oceanisediminis CGMCC 1.10115T (96.9 %), respectively.
30839250	11	55	theme	CGMCC	1386:1390	arg1	1.10115T					1392:1399	Bacillus oceanisediminis CGMCC 1.10115T	1361:1399	Bacillus oceanisediminis CGMCC 1.10115T with ANI and dDDH values of 75.2 and 12.8 %, respectively	1361:1457	The novel strain showed an average nucleotide identity (ANI) value of 77.8 % and a digital DNA-DNA hybridization (dDDH) value of 15.6 % with Falsibacillus pallidus CCTCC AB 207188T based on draft genome sequences, followed by Bacillus oceanisediminis CGMCC 1.10115T with ANI and dDDH values of 75.2 and 12.8 %, respectively.
30839250	14	56	theme	GY	1735:1736	arg1	10110T					1738:1743	GY 10110T	1735:1743	GY 10110T (=CGMCC 1.13648T=NBRC 113502T)	1735:1774	The type strain is GY 10110T (=CGMCC 1.13648T=NBRC 113502T).
30839250	14	56	theme	GY	1735:1736	arg1	113502T					1767:1773	=CGMCC 1.13648T=NBRC 113502T	1746:1773	=CGMCC 1.13648T=NBRC 113502T	1746:1773	The type strain is GY 10110T (=CGMCC 1.13648T=NBRC 113502T).
30839250	14	56	theme	GY	1735:1736	arg1	strain					1725:1730	The type strain	1716:1730	The type strain	1716:1730	The type strain is GY 10110T (=CGMCC 1.13648T=NBRC 113502T).
30839250	7	57	contain	contained	580:588	arg2	acid					610:613	meso-diaminopimelic acid	590:613	meso-diaminopimelic acid	590:613	The cell-wall peptidoglycan contained meso-diaminopimelic acid.
30839250	7	57	contain	contained	580:588	arg1	peptidoglycan					566:578	The cell-wall peptidoglycan	552:578	The cell-wall peptidoglycan	552:578	The cell-wall peptidoglycan contained meso-diaminopimelic acid.
30839250	11	58	theme	hybridization	1234:1246	arg1	value					1255:1259	a digital DNA-DNA hybridization (dDDH) value	1216:1259	a digital DNA-DNA hybridization (dDDH) value of 15.6 % with Falsibacillus pallidus CCTCC AB 207188T	1216:1314	The novel strain showed an average nucleotide identity (ANI) value of 77.8 % and a digital DNA-DNA hybridization (dDDH) value of 15.6 % with Falsibacillus pallidus CCTCC AB 207188T based on draft genome sequences, followed by Bacillus oceanisediminis CGMCC 1.10115T with ANI and dDDH values of 75.2 and 12.8 %, respectively.
30839250	12	59	dep	name	1669:1672	arg1	sp					1694:1695	Falsibacillus albus sp	1674:1695	the name Falsibacillus albus sp	1665:1695	The results of the polyphasic taxonomic study, including phenotypic, chemotaxonomic and phylogenetic analysis, showed that strain GY 10110T represents a novel species of the genus Falsibacillus, for which the name Falsibacillus albus sp.
30839250	11	60	dep	Falsibacillus	1276:1288	arg1	pallidus					1290:1297	pallidus	1290:1297	pallidus	1290:1297	The novel strain showed an average nucleotide identity (ANI) value of 77.8 % and a digital DNA-DNA hybridization (dDDH) value of 15.6 % with Falsibacillus pallidus CCTCC AB 207188T based on draft genome sequences, followed by Bacillus oceanisediminis CGMCC 1.10115T with ANI and dDDH values of 75.2 and 12.8 %, respectively.
30839250	11	61	theme	Bacillus	1361:1368	arg1	1.10115T					1392:1399	Bacillus oceanisediminis CGMCC 1.10115T	1361:1399	Bacillus oceanisediminis CGMCC 1.10115T with ANI and dDDH values of 75.2 and 12.8 %, respectively	1361:1457	The novel strain showed an average nucleotide identity (ANI) value of 77.8 % and a digital DNA-DNA hybridization (dDDH) value of 15.6 % with Falsibacillus pallidus CCTCC AB 207188T based on draft genome sequences, followed by Bacillus oceanisediminis CGMCC 1.10115T with ANI and dDDH values of 75.2 and 12.8 %, respectively.
30839250	8	62	theme	lipid	626:630	arg1	profile					632:638	The polar lipid profile	616:638	The polar lipid profile	616:638	The polar lipid profile comprised diphosphatidylglycerol, phosphatidylethanolamine, phosphoglycolipid, glycolipid, two unidentified aminophospholipids and three unidentified phospholipids.
30839250	5	63	theme	strain	446:451	arg1	10110T					456:461	strain GY 10110T	446:461	strain GY 10110T	446:461	The major fatty acids of strain GY 10110T were anteiso-C15 : 0, iso-C15 : 0 and iso-C16 : 0.
30839250	9	64	theme	sequence	960:967	arg1	similarity					969:978	98.0 % sequence similarity	953:978	98.0 % sequence similarity	953:978	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain GY 10110T was closely related to Falsibacillus pallidus CCTCC AB 207188T (98.0 % sequence similarity) and Bacillus oceanisediminis CGMCC 1.10115T (96.9 %), respectively.
30839250	9	64	theme	sequence	960:967	arg1	207188T					944:950	Falsibacillus pallidus CCTCC AB 207188T	912:950	Falsibacillus pallidus CCTCC AB 207188T (98.0 % sequence similarity)	912:979	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain GY 10110T was closely related to Falsibacillus pallidus CCTCC AB 207188T (98.0 % sequence similarity) and Bacillus oceanisediminis CGMCC 1.10115T (96.9 %), respectively.
30839250	12	65	theme	GY	1590:1591	arg1	10110T					1593:1598	strain GY 10110T	1583:1598	strain GY 10110T	1583:1598	The results of the polyphasic taxonomic study, including phenotypic, chemotaxonomic and phylogenetic analysis, showed that strain GY 10110T represents a novel species of the genus Falsibacillus, for which the name Falsibacillus albus sp.
30839250	9	66	dep	Falsibacillus	912:924	arg1	pallidus					926:933	pallidus	926:933	pallidus	926:933	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain GY 10110T was closely related to Falsibacillus pallidus CCTCC AB 207188T (98.0 % sequence similarity) and Bacillus oceanisediminis CGMCC 1.10115T (96.9 %), respectively.
30839250	5	67	theme	major	425:429	arg1	acids					437:441	The major fatty acids	421:441	The major fatty acids of strain GY 10110T	421:461	The major fatty acids of strain GY 10110T were anteiso-C15 : 0, iso-C15 : 0 and iso-C16 : 0.
30839250	5	67	theme	major	425:429	arg1	anteiso-C15 					468:479	anteiso-C15 	468:479	anteiso-C15 	468:479	The major fatty acids of strain GY 10110T were anteiso-C15 : 0, iso-C15 : 0 and iso-C16 : 0.
30839250	5	67	theme	major	425:429	arg1	 0					481:482	 0	481:482	 0	481:482	The major fatty acids of strain GY 10110T were anteiso-C15 : 0, iso-C15 : 0 and iso-C16 : 0.
30839250	12	68	theme	phylogenetic	1548:1559	arg1	analysis					1561:1568	phylogenetic analysis	1548:1568	phylogenetic analysis	1548:1568	The results of the polyphasic taxonomic study, including phenotypic, chemotaxonomic and phylogenetic analysis, showed that strain GY 10110T represents a novel species of the genus Falsibacillus, for which the name Falsibacillus albus sp.
30839250	9	69	theme	Bacillus	985:992	arg1	1.10115T					1016:1023	Bacillus oceanisediminis CGMCC 1.10115T	985:1023	Bacillus oceanisediminis CGMCC 1.10115T (96.9 %)	985:1032	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain GY 10110T was closely related to Falsibacillus pallidus CCTCC AB 207188T (98.0 % sequence similarity) and Bacillus oceanisediminis CGMCC 1.10115T (96.9 %), respectively.
30839250	9	69	theme	Bacillus	985:992	arg1	%					1031:1031	96.9 %	1026:1031	96.9 %	1026:1031	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain GY 10110T was closely related to Falsibacillus pallidus CCTCC AB 207188T (98.0 % sequence similarity) and Bacillus oceanisediminis CGMCC 1.10115T (96.9 %), respectively.
30839250	1	70	theme	mangrove	44:51	arg1	soil					53:56	mangrove soil	44:56	mangrove soil	44:56	nov., isolated from mangrove soil.
30839250	8	71	theme	unidentified	777:788	arg1	phospholipids					790:802	three unidentified phospholipids	771:802	three unidentified phospholipids	771:802	The polar lipid profile comprised diphosphatidylglycerol, phosphatidylethanolamine, phosphoglycolipid, glycolipid, two unidentified aminophospholipids and three unidentified phospholipids.
30839250	11	72	dep	Bacillus	1361:1368	arg1	oceanisediminis					1370:1384	oceanisediminis	1370:1384	oceanisediminis	1370:1384	The novel strain showed an average nucleotide identity (ANI) value of 77.8 % and a digital DNA-DNA hybridization (dDDH) value of 15.6 % with Falsibacillus pallidus CCTCC AB 207188T based on draft genome sequences, followed by Bacillus oceanisediminis CGMCC 1.10115T with ANI and dDDH values of 75.2 and 12.8 %, respectively.
30839250	9	73	theme	CGMCC	1010:1014	arg1	1.10115T					1016:1023	Bacillus oceanisediminis CGMCC 1.10115T	985:1023	Bacillus oceanisediminis CGMCC 1.10115T (96.9 %)	985:1032	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain GY 10110T was closely related to Falsibacillus pallidus CCTCC AB 207188T (98.0 % sequence similarity) and Bacillus oceanisediminis CGMCC 1.10115T (96.9 %), respectively.
30839250	9	73	theme	CGMCC	1010:1014	arg1	%					1031:1031	96.9 %	1026:1031	96.9 %	1026:1031	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain GY 10110T was closely related to Falsibacillus pallidus CCTCC AB 207188T (98.0 % sequence similarity) and Bacillus oceanisediminis CGMCC 1.10115T (96.9 %), respectively.
30839250	3	74	with	motile	272:277	arg1	flagella					297:304	peritrichous flagella	284:304	peritrichous flagella	284:304	Cells were aerobic, motile with peritrichous flagella and rod-shaped.
30839250	11	75	theme	%	1210:1210	arg1	value					1255:1259	a digital DNA-DNA hybridization (dDDH) value	1216:1259	a digital DNA-DNA hybridization (dDDH) value of 15.6 % with Falsibacillus pallidus CCTCC AB 207188T	1216:1314	The novel strain showed an average nucleotide identity (ANI) value of 77.8 % and a digital DNA-DNA hybridization (dDDH) value of 15.6 % with Falsibacillus pallidus CCTCC AB 207188T based on draft genome sequences, followed by Bacillus oceanisediminis CGMCC 1.10115T with ANI and dDDH values of 75.2 and 12.8 %, respectively.
30839250	11	75	theme	%	1210:1210	arg1	value					1196:1200	an average nucleotide identity (ANI) value	1159:1200	an average nucleotide identity (ANI) value of 77.8 %	1159:1210	The novel strain showed an average nucleotide identity (ANI) value of 77.8 % and a digital DNA-DNA hybridization (dDDH) value of 15.6 % with Falsibacillus pallidus CCTCC AB 207188T based on draft genome sequences, followed by Bacillus oceanisediminis CGMCC 1.10115T with ANI and dDDH values of 75.2 and 12.8 %, respectively.
30839250	11	76	theme	AB	1305:1306	arg1	207188T					1308:1314	Falsibacillus pallidus CCTCC AB 207188T	1276:1314	Falsibacillus pallidus CCTCC AB 207188T	1276:1314	The novel strain showed an average nucleotide identity (ANI) value of 77.8 % and a digital DNA-DNA hybridization (dDDH) value of 15.6 % with Falsibacillus pallidus CCTCC AB 207188T based on draft genome sequences, followed by Bacillus oceanisediminis CGMCC 1.10115T with ANI and dDDH values of 75.2 and 12.8 %, respectively.
30839250	5	77	theme	10110T	456:461	arg1	acids					437:441	The major fatty acids	421:441	The major fatty acids of strain GY 10110T	421:461	The major fatty acids of strain GY 10110T were anteiso-C15 : 0, iso-C15 : 0 and iso-C16 : 0.
30839250	5	77	theme	10110T	456:461	arg1	anteiso-C15 					468:479	anteiso-C15 	468:479	anteiso-C15 	468:479	The major fatty acids of strain GY 10110T were anteiso-C15 : 0, iso-C15 : 0 and iso-C16 : 0.
30839250	5	77	theme	10110T	456:461	arg1	 0					481:482	 0	481:482	 0	481:482	The major fatty acids of strain GY 10110T were anteiso-C15 : 0, iso-C15 : 0 and iso-C16 : 0.
30839250	4	78	dep	15-37 °C	341:348	arg1	28 °C					360:364	28 °C	360:364	28 °C	360:364	The strain grew at 15-37 °C (optimum, 28 °C), at 0-3 %(w/v) NaCl (1 %) and at pH 6.0-9.0 (pH 7.0).
30839250	4	78	dep	15-37 °C	341:348	arg1	optimum					351:357	optimum	351:357	optimum	351:357	The strain grew at 15-37 °C (optimum, 28 °C), at 0-3 %(w/v) NaCl (1 %) and at pH 6.0-9.0 (pH 7.0).
30839250	12	79	theme	taxonomic	1490:1498	arg1	study					1500:1504	the polyphasic taxonomic study	1475:1504	the polyphasic taxonomic study	1475:1504	The results of the polyphasic taxonomic study, including phenotypic, chemotaxonomic and phylogenetic analysis, showed that strain GY 10110T represents a novel species of the genus Falsibacillus, for which the name Falsibacillus albus sp.
30839250	9	80	theme	Phylogenetic	805:816	arg1	analysis					818:825	Phylogenetic analysis	805:825	Phylogenetic analysis based on 16S rRNA gene sequences	805:858	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain GY 10110T was closely related to Falsibacillus pallidus CCTCC AB 207188T (98.0 % sequence similarity) and Bacillus oceanisediminis CGMCC 1.10115T (96.9 %), respectively.
30839250	11	81	theme	draft	1325:1329	arg1	sequences					1338:1346	draft genome sequences	1325:1346	draft genome sequences	1325:1346	The novel strain showed an average nucleotide identity (ANI) value of 77.8 % and a digital DNA-DNA hybridization (dDDH) value of 15.6 % with Falsibacillus pallidus CCTCC AB 207188T based on draft genome sequences, followed by Bacillus oceanisediminis CGMCC 1.10115T with ANI and dDDH values of 75.2 and 12.8 %, respectively.
30839250	8	82	theme	polar	620:624	arg1	profile					632:638	The polar lipid profile	616:638	The polar lipid profile	616:638	The polar lipid profile comprised diphosphatidylglycerol, phosphatidylethanolamine, phosphoglycolipid, glycolipid, two unidentified aminophospholipids and three unidentified phospholipids.
30839250	11	83	theme	nucleotide	1170:1179	arg1	value					1196:1200	an average nucleotide identity (ANI) value	1159:1200	an average nucleotide identity (ANI) value of 77.8 %	1159:1210	The novel strain showed an average nucleotide identity (ANI) value of 77.8 % and a digital DNA-DNA hybridization (dDDH) value of 15.6 % with Falsibacillus pallidus CCTCC AB 207188T based on draft genome sequences, followed by Bacillus oceanisediminis CGMCC 1.10115T with ANI and dDDH values of 75.2 and 12.8 %, respectively.
30839250	10	84	theme	strain	1068:1073	arg1	10110T					1078:1083	strain GY 10110T	1068:1083	strain GY 10110T	1068:1083	The G+C content of strain GY 10110T based on the whole genome sequence was 42.3 mol%.
30839250	12	85	theme	albus	1688:1692	arg1	sp					1694:1695	Falsibacillus albus sp	1674:1695	the name Falsibacillus albus sp	1665:1695	The results of the polyphasic taxonomic study, including phenotypic, chemotaxonomic and phylogenetic analysis, showed that strain GY 10110T represents a novel species of the genus Falsibacillus, for which the name Falsibacillus albus sp.
30839250	9	86	theme	gene	845:848	arg1	sequences					850:858	16S rRNA gene sequences	836:858	16S rRNA gene sequences	836:858	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain GY 10110T was closely related to Falsibacillus pallidus CCTCC AB 207188T (98.0 % sequence similarity) and Bacillus oceanisediminis CGMCC 1.10115T (96.9 %), respectively.
30839250	2	87	theme	mangrove	189:196	arg1	soil					198:201	mangrove soil	189:201	mangrove soil collected from Qinzhou, Guangxi province, China	189:249	A novel Gram-stain-positive, catalase- and oxidase-positive, endospore-forming bacterium, designated GY 10110T, was isolated from mangrove soil collected from Qinzhou, Guangxi province, China.
30839250	10	88	theme	whole	1098:1102	arg1	sequence					1111:1118	the whole genome sequence	1094:1118	the whole genome sequence	1094:1118	The G+C content of strain GY 10110T based on the whole genome sequence was 42.3 mol%.
30839250	5	89	dep	 0	481:482	arg1	 0					510:511	 0	510:511	 0	510:511	The major fatty acids of strain GY 10110T were anteiso-C15 : 0, iso-C15 : 0 and iso-C16 : 0.
30839250	5	89	dep	 0	481:482	arg1	iso-C16 					501:508	iso-C16 	501:508	iso-C16 	501:508	The major fatty acids of strain GY 10110T were anteiso-C15 : 0, iso-C15 : 0 and iso-C16 : 0.
30839250	5	89	dep	 0	481:482	arg1	 0					494:495	 0	494:495	 0	494:495	The major fatty acids of strain GY 10110T were anteiso-C15 : 0, iso-C15 : 0 and iso-C16 : 0.
30839250	11	90	theme	%	1269:1269	arg1	value					1255:1259	a digital DNA-DNA hybridization (dDDH) value	1216:1259	a digital DNA-DNA hybridization (dDDH) value of 15.6 % with Falsibacillus pallidus CCTCC AB 207188T	1216:1314	The novel strain showed an average nucleotide identity (ANI) value of 77.8 % and a digital DNA-DNA hybridization (dDDH) value of 15.6 % with Falsibacillus pallidus CCTCC AB 207188T based on draft genome sequences, followed by Bacillus oceanisediminis CGMCC 1.10115T with ANI and dDDH values of 75.2 and 12.8 %, respectively.
30839250	11	90	theme	%	1269:1269	arg1	value					1196:1200	an average nucleotide identity (ANI) value	1159:1200	an average nucleotide identity (ANI) value of 77.8 %	1159:1210	The novel strain showed an average nucleotide identity (ANI) value of 77.8 % and a digital DNA-DNA hybridization (dDDH) value of 15.6 % with Falsibacillus pallidus CCTCC AB 207188T based on draft genome sequences, followed by Bacillus oceanisediminis CGMCC 1.10115T with ANI and dDDH values of 75.2 and 12.8 %, respectively.
30839250	2	91	theme	endospore-forming	120:136	arg1	10110T					163:168	designated GY 10110T	149:168	designated GY 10110T	149:168	A novel Gram-stain-positive, catalase- and oxidase-positive, endospore-forming bacterium, designated GY 10110T, was isolated from mangrove soil collected from Qinzhou, Guangxi province, China.
30839250	2	91	theme	endospore-forming	120:136	arg1	bacterium					138:146	A novel Gram-stain-positive, catalase- and oxidase-positive, endospore-forming bacterium	59:146	bacterium	138:146	A novel Gram-stain-positive, catalase- and oxidase-positive, endospore-forming bacterium, designated GY 10110T, was isolated from mangrove soil collected from Qinzhou, Guangxi province, China.
30839250	11	92	with	%	1269:1269	arg1	207188T					1308:1314	Falsibacillus pallidus CCTCC AB 207188T	1276:1314	Falsibacillus pallidus CCTCC AB 207188T	1276:1314	The novel strain showed an average nucleotide identity (ANI) value of 77.8 % and a digital DNA-DNA hybridization (dDDH) value of 15.6 % with Falsibacillus pallidus CCTCC AB 207188T based on draft genome sequences, followed by Bacillus oceanisediminis CGMCC 1.10115T with ANI and dDDH values of 75.2 and 12.8 %, respectively.
30215596	5	0	theme	Phylogenetic	525:536	arg1	analyses					538:545	Phylogenetic analyses	525:545	Phylogenetic analyses based on 16S rRNA gene sequences	525:578	Phylogenetic analyses based on 16S rRNA gene sequences represented that the isolate belongs to the genus Blastococcus.
30215596	11	1	dep	48.6 	1401:1405	arg1	to					1398:1399	to	1398:1399	to	1398:1399	DNA-DNA relatedness values between strain GP-S2-8T and type strains of the genus Blastococcus ranged from 14.6 to 48.6 %.
30215596	2	2	theme	bacterial	118:126	arg1	strain					128:133	A novel Gram-strain-positive, non-spore-forming bacterial strain	70:133	A novel Gram-strain-positive, non-spore-forming bacterial strain	70:133	A novel Gram-strain-positive, non-spore-forming bacterial strain, designated GP-S2-8T, was isolated from a sea-tidal flat sediment sample from Gopado, Republic of Korea.
30215596	6	3	theme	meso-diaminopimelic	707:725	arg1	acid					727:730	meso-diaminopimelic acid	707:730	meso-diaminopimelic acid	707:730	The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid.
30215596	6	3	theme	meso-diaminopimelic	707:725	arg1	acid					667:670	The diagnostic diamino acid	644:670	The diagnostic diamino acid in the cell-wall peptidoglycan	644:701	The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid.
30215596	9	4	theme	lipid	1039:1043	arg1	profile					1045:1051	The polar lipid profile	1029:1051	The polar lipid profile	1029:1051	The polar lipid profile included diphosphadidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol, one unidentified glycophospholipid, two unidentified phospholipids and five unidentified lipids.
30215596	8	5	dep	 0	932:933	arg1	iso-C14 					1016:1023	iso-C14 	1016:1023	iso-C14 	1016:1023	The predominant respiratory quinone was MK-9(H4) and the major fatty acids were iso-C16 : 0, iso-C16 : 1 H, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0 and iso-C14 : 0.
30215596	8	5	dep	 0	932:933	arg1	C16 					969:972	C16 	969:972	C16 	969:972	The predominant respiratory quinone was MK-9(H4) and the major fatty acids were iso-C16 : 0, iso-C16 : 1 H, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0 and iso-C14 : 0.
30215596	8	5	dep	 0	932:933	arg1	 0					1025:1026	 0	1025:1026	 0	1025:1026	The predominant respiratory quinone was MK-9(H4) and the major fatty acids were iso-C16 : 0, iso-C16 : 1 H, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0 and iso-C14 : 0.
30215596	8	5	dep	 0	932:933	arg1	feature					958:964	summed feature 3	951:966	summed feature 3	951:966	The predominant respiratory quinone was MK-9(H4) and the major fatty acids were iso-C16 : 0, iso-C16 : 1 H, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0 and iso-C14 : 0.
30215596	8	5	dep	 0	932:933	arg1	 0					1009:1010	 0	1009:1010	 0	1009:1010	The predominant respiratory quinone was MK-9(H4) and the major fatty acids were iso-C16 : 0, iso-C16 : 1 H, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0 and iso-C14 : 0.
30215596	8	5	dep	 0	932:933	arg1	 1 H					945:948	 1 H	945:948	 1 H	945:948	The predominant respiratory quinone was MK-9(H4) and the major fatty acids were iso-C16 : 0, iso-C16 : 1 H, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0 and iso-C14 : 0.
30215596	2	6	dep	Gram-strain-positive	78:97	arg1	non-spore-forming					100:116	non-spore-forming	100:116	non-spore-forming	100:116	A novel Gram-strain-positive, non-spore-forming bacterial strain, designated GP-S2-8T, was isolated from a sea-tidal flat sediment sample from Gopado, Republic of Korea.
30215596	10	7	theme	DNA	1256:1258	arg1	G+C content					1260:1270	The DNA G+C content	1252:1270	The DNA G+C content	1252:1270	The DNA G+C content was 74.2 mol%.
30215596	10	7	theme	DNA	1256:1258	arg1	%					1284:1284	74.2 mol%	1276:1284	74.2 mol%	1276:1284	The DNA G+C content was 74.2 mol%.
30215596	1	8	theme	sea-tidal	45:53	arg1	sediment					60:67	sea-tidal flat sediment	45:67	sea-tidal flat sediment	45:67	nov., isolated from sea-tidal flat sediment.
30215596	11	9	theme	relatedness	1295:1305	arg1	values					1307:1312	DNA-DNA relatedness values	1287:1312	DNA-DNA relatedness values between strain GP-S2-8T and type strains of the genus Blastococcus	1287:1379	DNA-DNA relatedness values between strain GP-S2-8T and type strains of the genus Blastococcus ranged from 14.6 to 48.6 %.
30215596	9	10	theme	unidentified	1159:1170	arg1	glycophospholipid					1172:1188	one unidentified glycophospholipid	1155:1188	one unidentified glycophospholipid	1155:1188	The polar lipid profile included diphosphadidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol, one unidentified glycophospholipid, two unidentified phospholipids and five unidentified lipids.
30215596	9	10	theme	unidentified	1159:1170	arg1	diphosphadidylglycerol					1062:1083	diphosphadidylglycerol	1062:1083	diphosphadidylglycerol	1062:1083	The polar lipid profile included diphosphadidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol, one unidentified glycophospholipid, two unidentified phospholipids and five unidentified lipids.
30215596	1	11	theme	flat	55:58	arg1	sediment					60:67	sea-tidal flat sediment	45:67	sea-tidal flat sediment	45:67	nov., isolated from sea-tidal flat sediment.
30215596	7	12	theme	strain	762:767	arg1	GP-S2-8T					769:776	strain GP-S2-8T	762:776	strain GP-S2-8T	762:776	Whole-cell sugar analysis of strain GP-S2-8T revealed rhamnose, glucose and mannose as characteristic sugars.
30215596	4	13	theme	%	503:503	arg1	NaCl					511:514	0-11 % (w/v) NaCl	498:514	0-11 % (w/v) NaCl (0-3 %)	498:522	The strain grew at 4-45 °C (optimum, 28-37 °C), at pH 4.0-11.0 (pH 7.0-9.0) and in the presence of 0-11 % (w/v) NaCl (0-3 %).
30215596	4	13	theme	%	503:503	arg1	%					521:521	0-3 %	517:521	0-3 %	517:521	The strain grew at 4-45 °C (optimum, 28-37 °C), at pH 4.0-11.0 (pH 7.0-9.0) and in the presence of 0-11 % (w/v) NaCl (0-3 %).
30215596	11	14	theme	strain	1322:1327	arg1	GP-S2-8T					1329:1336	strain GP-S2-8T and type strains	1322:1353	GP-S2-8T	1329:1336	DNA-DNA relatedness values between strain GP-S2-8T and type strains of the genus Blastococcus ranged from 14.6 to 48.6 %.
30215596	6	15	theme	cell-wall	679:687	arg1	peptidoglycan					689:701	the cell-wall peptidoglycan	675:701	the cell-wall peptidoglycan	675:701	The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid.
30215596	7	16	theme	GP-S2-8T	769:776	arg1	analysis					750:757	Whole-cell sugar analysis	733:757	Whole-cell sugar analysis of strain GP-S2-8T	733:776	Whole-cell sugar analysis of strain GP-S2-8T revealed rhamnose, glucose and mannose as characteristic sugars.
30215596	5	17	theme	genus	624:628	arg1	Blastococcus					630:641	the genus Blastococcus	620:641	the genus Blastococcus	620:641	Phylogenetic analyses based on 16S rRNA gene sequences represented that the isolate belongs to the genus Blastococcus.
30215596	8	18	theme	major	900:904	arg1	iso-C16 					923:930	iso-C16 	923:930	iso-C16 	923:930	The predominant respiratory quinone was MK-9(H4) and the major fatty acids were iso-C16 : 0, iso-C16 : 1 H, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0 and iso-C14 : 0.
30215596	8	18	theme	major	900:904	arg1	acids					912:916	the major fatty acids	896:916	the major fatty acids	896:916	The predominant respiratory quinone was MK-9(H4) and the major fatty acids were iso-C16 : 0, iso-C16 : 1 H, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0 and iso-C14 : 0.
30215596	12	19	theme	Blastococcus	1532:1543	arg1	species					1511:1517	a new species	1505:1517	a new species	1505:1517	On the basis of the phenotypic differences and DNA-DNA relatedness data, the isolate represents a new species of the genus Blastococcus, for which the name Blastococcuslitoris sp.
30215596	12	20	theme	relatedness	1464:1474	arg1	data					1476:1479	DNA-DNA relatedness data	1456:1479	DNA-DNA relatedness data	1456:1479	On the basis of the phenotypic differences and DNA-DNA relatedness data, the isolate represents a new species of the genus Blastococcus, for which the name Blastococcuslitoris sp.
30215596	0	21	theme	litoris	13:19	arg1	sp					21:22	Blastococcus litoris sp	0:22	Blastococcus litoris sp.	0:23	Blastococcus litoris sp.
30215596	14	22	theme	32354T=DSM	1653:1662	arg1	GP-S2-8T					1626:1633	GP-S2-8T	1626:1633	GP-S2-8T (=KCCM 43275T=JCM 32354T=DSM 106127T=KCTC 49078T)	1626:1683	The type strain is GP-S2-8T (=KCCM 43275T=JCM 32354T=DSM 106127T=KCTC 49078T).
30215596	14	22	theme	32354T=DSM	1653:1662	arg1	49078T					1677:1682	=KCCM 43275T=JCM 32354T=DSM 106127T=KCTC 49078T	1636:1682	=KCCM 43275T=JCM 32354T=DSM 106127T=KCTC 49078T	1636:1682	The type strain is GP-S2-8T (=KCCM 43275T=JCM 32354T=DSM 106127T=KCTC 49078T).
30215596	12	23	theme	name	1560:1563	arg1	sp					1585:1586	the name Blastococcuslitoris sp	1556:1586	the name Blastococcuslitoris sp	1556:1586	On the basis of the phenotypic differences and DNA-DNA relatedness data, the isolate represents a new species of the genus Blastococcus, for which the name Blastococcuslitoris sp.
30215596	2	24	from	Republic	221:228	arg1	sample					201:206	a sea-tidal flat sediment sample	175:206	a sea-tidal flat sediment sample from Gopado, Republic of Korea	175:237	A novel Gram-strain-positive, non-spore-forming bacterial strain, designated GP-S2-8T, was isolated from a sea-tidal flat sediment sample from Gopado, Republic of Korea.
30215596	14	25	theme	43275T=JCM	1642:1651	arg1	GP-S2-8T					1626:1633	GP-S2-8T	1626:1633	GP-S2-8T (=KCCM 43275T=JCM 32354T=DSM 106127T=KCTC 49078T)	1626:1683	The type strain is GP-S2-8T (=KCCM 43275T=JCM 32354T=DSM 106127T=KCTC 49078T).
30215596	14	25	theme	43275T=JCM	1642:1651	arg1	49078T					1677:1682	=KCCM 43275T=JCM 32354T=DSM 106127T=KCTC 49078T	1636:1682	=KCCM 43275T=JCM 32354T=DSM 106127T=KCTC 49078T	1636:1682	The type strain is GP-S2-8T (=KCCM 43275T=JCM 32354T=DSM 106127T=KCTC 49078T).
30215596	0	26	theme	Blastococcus	0:11	arg1	sp					21:22	Blastococcus litoris sp	0:22	Blastococcus litoris sp.	0:23	Blastococcus litoris sp.
30215596	9	27	theme	unidentified	1195:1206	arg1	diphosphadidylglycerol					1062:1083	diphosphadidylglycerol	1062:1083	diphosphadidylglycerol	1062:1083	The polar lipid profile included diphosphadidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol, one unidentified glycophospholipid, two unidentified phospholipids and five unidentified lipids.
30215596	9	27	theme	unidentified	1195:1206	arg1	phospholipids					1208:1220	two unidentified phospholipids	1191:1220	two unidentified phospholipids	1191:1220	The polar lipid profile included diphosphadidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol, one unidentified glycophospholipid, two unidentified phospholipids and five unidentified lipids.
30215596	7	28	theme	Whole-cell	733:742	arg1	analysis					750:757	Whole-cell sugar analysis	733:757	Whole-cell sugar analysis of strain GP-S2-8T	733:776	Whole-cell sugar analysis of strain GP-S2-8T revealed rhamnose, glucose and mannose as characteristic sugars.
30215596	8	29	dep	C16 	969:972	arg1	 1ω6c					992:996	 1ω6c	992:996	 1ω6c	992:996	The predominant respiratory quinone was MK-9(H4) and the major fatty acids were iso-C16 : 0, iso-C16 : 1 H, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0 and iso-C14 : 0.
30215596	8	29	dep	C16 	969:972	arg1	C16 					987:990	C16 	987:990	C16 	987:990	The predominant respiratory quinone was MK-9(H4) and the major fatty acids were iso-C16 : 0, iso-C16 : 1 H, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0 and iso-C14 : 0.
30215596	8	29	dep	C16 	969:972	arg1	 1ω7c					974:978	 1ω7c	974:978	 1ω7c	974:978	The predominant respiratory quinone was MK-9(H4) and the major fatty acids were iso-C16 : 0, iso-C16 : 1 H, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0 and iso-C14 : 0.
30215596	8	30	theme	summed	951:956	arg1	feature					958:964	summed feature 3	951:966	summed feature 3	951:966	The predominant respiratory quinone was MK-9(H4) and the major fatty acids were iso-C16 : 0, iso-C16 : 1 H, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0 and iso-C14 : 0.
30215596	8	30	theme	summed	951:956	arg1	 1 H					945:948	 1 H	945:948	 1 H	945:948	The predominant respiratory quinone was MK-9(H4) and the major fatty acids were iso-C16 : 0, iso-C16 : 1 H, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0 and iso-C14 : 0.
30215596	6	31	theme	diamino	659:665	arg1	acid					727:730	meso-diaminopimelic acid	707:730	meso-diaminopimelic acid	707:730	The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid.
30215596	6	31	theme	diamino	659:665	arg1	acid					667:670	The diagnostic diamino acid	644:670	The diagnostic diamino acid in the cell-wall peptidoglycan	644:701	The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid.
30215596	7	32	theme	sugar	744:748	arg1	analysis					750:757	Whole-cell sugar analysis	733:757	Whole-cell sugar analysis of strain GP-S2-8T	733:776	Whole-cell sugar analysis of strain GP-S2-8T revealed rhamnose, glucose and mannose as characteristic sugars.
30215596	6	33	from	acid	667:670	arg1	peptidoglycan					689:701	the cell-wall peptidoglycan	675:701	the cell-wall peptidoglycan	675:701	The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid.
30215596	14	34	theme	106127T=KCTC	1664:1675	arg1	GP-S2-8T					1626:1633	GP-S2-8T	1626:1633	GP-S2-8T (=KCCM 43275T=JCM 32354T=DSM 106127T=KCTC 49078T)	1626:1683	The type strain is GP-S2-8T (=KCCM 43275T=JCM 32354T=DSM 106127T=KCTC 49078T).
30215596	14	34	theme	106127T=KCTC	1664:1675	arg1	49078T					1677:1682	=KCCM 43275T=JCM 32354T=DSM 106127T=KCTC 49078T	1636:1682	=KCCM 43275T=JCM 32354T=DSM 106127T=KCTC 49078T	1636:1682	The type strain is GP-S2-8T (=KCCM 43275T=JCM 32354T=DSM 106127T=KCTC 49078T).
30215596	2	35	theme	sea-tidal	177:185	arg1	sample					201:206	a sea-tidal flat sediment sample	175:206	a sea-tidal flat sediment sample from Gopado, Republic of Korea	175:237	A novel Gram-strain-positive, non-spore-forming bacterial strain, designated GP-S2-8T, was isolated from a sea-tidal flat sediment sample from Gopado, Republic of Korea.
30215596	12	36	theme	new	1507:1509	arg1	species					1511:1517	a new species	1505:1517	a new species	1505:1517	On the basis of the phenotypic differences and DNA-DNA relatedness data, the isolate represents a new species of the genus Blastococcus, for which the name Blastococcuslitoris sp.
30215596	12	37	theme	genus	1526:1530	arg1	Blastococcus					1532:1543	the genus Blastococcus	1522:1543	the genus Blastococcus	1522:1543	On the basis of the phenotypic differences and DNA-DNA relatedness data, the isolate represents a new species of the genus Blastococcus, for which the name Blastococcuslitoris sp.
30215596	4	38	dep	optimum	427:433	arg1	28-37 °C					436:443	28-37 °C	436:443	28-37 °C	436:443	The strain grew at 4-45 °C (optimum, 28-37 °C), at pH 4.0-11.0 (pH 7.0-9.0) and in the presence of 0-11 % (w/v) NaCl (0-3 %).
30215596	14	39	theme	=KCCM	1636:1640	arg1	GP-S2-8T					1626:1633	GP-S2-8T	1626:1633	GP-S2-8T (=KCCM 43275T=JCM 32354T=DSM 106127T=KCTC 49078T)	1626:1683	The type strain is GP-S2-8T (=KCCM 43275T=JCM 32354T=DSM 106127T=KCTC 49078T).
30215596	14	39	theme	=KCCM	1636:1640	arg1	49078T					1677:1682	=KCCM 43275T=JCM 32354T=DSM 106127T=KCTC 49078T	1636:1682	=KCCM 43275T=JCM 32354T=DSM 106127T=KCTC 49078T	1636:1682	The type strain is GP-S2-8T (=KCCM 43275T=JCM 32354T=DSM 106127T=KCTC 49078T).
30215596	11	40	theme	type	1342:1345	arg1	strains					1347:1353	strain GP-S2-8T and type strains	1322:1353	strains	1347:1353	DNA-DNA relatedness values between strain GP-S2-8T and type strains of the genus Blastococcus ranged from 14.6 to 48.6 %.
30215596	2	41	attach	isolated	161:168	arg1	sample					201:206	a sea-tidal flat sediment sample	175:206	a sea-tidal flat sediment sample from Gopado, Republic of Korea	175:237	A novel Gram-strain-positive, non-spore-forming bacterial strain, designated GP-S2-8T, was isolated from a sea-tidal flat sediment sample from Gopado, Republic of Korea.
30215596	2	41	attach	isolated	161:168	arg2	strain					128:133	A novel Gram-strain-positive, non-spore-forming bacterial strain	70:133	A novel Gram-strain-positive, non-spore-forming bacterial strain	70:133	A novel Gram-strain-positive, non-spore-forming bacterial strain, designated GP-S2-8T, was isolated from a sea-tidal flat sediment sample from Gopado, Republic of Korea.
30215596	11	42	theme	Blastococcus	1368:1379	arg1	GP-S2-8T					1329:1336	strain GP-S2-8T and type strains	1322:1353	GP-S2-8T	1329:1336	DNA-DNA relatedness values between strain GP-S2-8T and type strains of the genus Blastococcus ranged from 14.6 to 48.6 %.
30215596	11	42	theme	Blastococcus	1368:1379	arg1	strains					1347:1353	strain GP-S2-8T and type strains	1322:1353	strains	1347:1353	DNA-DNA relatedness values between strain GP-S2-8T and type strains of the genus Blastococcus ranged from 14.6 to 48.6 %.
30215596	9	43	theme	unidentified	1231:1242	arg1	lipids					1244:1249	five unidentified lipids	1226:1249	five unidentified lipids	1226:1249	The polar lipid profile included diphosphadidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol, one unidentified glycophospholipid, two unidentified phospholipids and five unidentified lipids.
30215596	9	43	theme	unidentified	1231:1242	arg1	diphosphadidylglycerol					1062:1083	diphosphadidylglycerol	1062:1083	diphosphadidylglycerol	1062:1083	The polar lipid profile included diphosphadidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol, one unidentified glycophospholipid, two unidentified phospholipids and five unidentified lipids.
30215596	14	44	theme	type	1611:1614	arg1	GP-S2-8T					1626:1633	GP-S2-8T	1626:1633	GP-S2-8T (=KCCM 43275T=JCM 32354T=DSM 106127T=KCTC 49078T)	1626:1683	The type strain is GP-S2-8T (=KCCM 43275T=JCM 32354T=DSM 106127T=KCTC 49078T).
30215596	14	44	theme	type	1611:1614	arg1	strain					1616:1621	The type strain	1607:1621	The type strain	1607:1621	The type strain is GP-S2-8T (=KCCM 43275T=JCM 32354T=DSM 106127T=KCTC 49078T).
30215596	6	45	theme	diagnostic	648:657	arg1	acid					727:730	meso-diaminopimelic acid	707:730	meso-diaminopimelic acid	707:730	The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid.
30215596	6	45	theme	diagnostic	648:657	arg1	acid					667:670	The diagnostic diamino acid	644:670	The diagnostic diamino acid in the cell-wall peptidoglycan	644:701	The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid.
30215596	12	46	theme	DNA-DNA	1456:1462	arg1	data					1476:1479	DNA-DNA relatedness data	1456:1479	DNA-DNA relatedness data	1456:1479	On the basis of the phenotypic differences and DNA-DNA relatedness data, the isolate represents a new species of the genus Blastococcus, for which the name Blastococcuslitoris sp.
30215596	2	47	theme	Gram-strain-positive	78:97	arg1	strain					128:133	A novel Gram-strain-positive, non-spore-forming bacterial strain	70:133	A novel Gram-strain-positive, non-spore-forming bacterial strain	70:133	A novel Gram-strain-positive, non-spore-forming bacterial strain, designated GP-S2-8T, was isolated from a sea-tidal flat sediment sample from Gopado, Republic of Korea.
30215596	5	48	theme	16S	556:558	arg1	sequences					570:578	16S rRNA gene sequences	556:578	16S rRNA gene sequences	556:578	Phylogenetic analyses based on 16S rRNA gene sequences represented that the isolate belongs to the genus Blastococcus.
30215596	10	49	theme	74.2 mol	1276:1283	arg1	G+C content					1260:1270	The DNA G+C content	1252:1270	The DNA G+C content	1252:1270	The DNA G+C content was 74.2 mol%.
30215596	10	49	theme	74.2 mol	1276:1283	arg1	%					1284:1284	74.2 mol%	1276:1284	74.2 mol%	1276:1284	The DNA G+C content was 74.2 mol%.
30215596	9	50	theme	polar	1033:1037	arg1	profile					1045:1051	The polar lipid profile	1029:1051	The polar lipid profile	1029:1051	The polar lipid profile included diphosphadidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol, one unidentified glycophospholipid, two unidentified phospholipids and five unidentified lipids.
30215596	12	51	dep	differences	1440:1450	arg1	the					1412:1414	the	1412:1414	the	1412:1414	On the basis of the phenotypic differences and DNA-DNA relatedness data, the isolate represents a new species of the genus Blastococcus, for which the name Blastococcuslitoris sp.
30215596	12	51	dep	differences	1440:1450	arg1	basis					1416:1420	basis	1416:1420	basis	1416:1420	On the basis of the phenotypic differences and DNA-DNA relatedness data, the isolate represents a new species of the genus Blastococcus, for which the name Blastococcuslitoris sp.
30215596	2	52	theme	novel	72:76	arg1	strain					128:133	A novel Gram-strain-positive, non-spore-forming bacterial strain	70:133	A novel Gram-strain-positive, non-spore-forming bacterial strain	70:133	A novel Gram-strain-positive, non-spore-forming bacterial strain, designated GP-S2-8T, was isolated from a sea-tidal flat sediment sample from Gopado, Republic of Korea.
30215596	5	53	theme	rRNA	560:563	arg1	sequences					570:578	16S rRNA gene sequences	556:578	16S rRNA gene sequences	556:578	Phylogenetic analyses based on 16S rRNA gene sequences represented that the isolate belongs to the genus Blastococcus.
30215596	11	54	theme	DNA-DNA	1287:1293	arg1	values					1307:1312	DNA-DNA relatedness values	1287:1312	DNA-DNA relatedness values between strain GP-S2-8T and type strains of the genus Blastococcus	1287:1379	DNA-DNA relatedness values between strain GP-S2-8T and type strains of the genus Blastococcus ranged from 14.6 to 48.6 %.
30215596	8	55	theme	predominant	847:857	arg1	quinone					871:877	The predominant respiratory quinone	843:877	The predominant respiratory quinone	843:877	The predominant respiratory quinone was MK-9(H4) and the major fatty acids were iso-C16 : 0, iso-C16 : 1 H, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0 and iso-C14 : 0.
30215596	8	55	theme	predominant	847:857	arg1	MK-9					883:886	MK-9	883:886	MK-9	883:886	The predominant respiratory quinone was MK-9(H4) and the major fatty acids were iso-C16 : 0, iso-C16 : 1 H, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0 and iso-C14 : 0.
30215596	8	56	dep	MK-9	883:886	arg1	 0					932:933	 0	932:933	 0	932:933	The predominant respiratory quinone was MK-9(H4) and the major fatty acids were iso-C16 : 0, iso-C16 : 1 H, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0 and iso-C14 : 0.
30215596	8	57	theme	fatty	906:910	arg1	iso-C16 					923:930	iso-C16 	923:930	iso-C16 	923:930	The predominant respiratory quinone was MK-9(H4) and the major fatty acids were iso-C16 : 0, iso-C16 : 1 H, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0 and iso-C14 : 0.
30215596	8	57	theme	fatty	906:910	arg1	acids					912:916	the major fatty acids	896:916	the major fatty acids	896:916	The predominant respiratory quinone was MK-9(H4) and the major fatty acids were iso-C16 : 0, iso-C16 : 1 H, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0 and iso-C14 : 0.
30215596	5	58	theme	gene	565:568	arg1	sequences					570:578	16S rRNA gene sequences	556:578	16S rRNA gene sequences	556:578	Phylogenetic analyses based on 16S rRNA gene sequences represented that the isolate belongs to the genus Blastococcus.
30215596	12	59	theme	Blastococcuslitoris	1565:1583	arg1	sp					1585:1586	the name Blastococcuslitoris sp	1556:1586	the name Blastococcuslitoris sp	1556:1586	On the basis of the phenotypic differences and DNA-DNA relatedness data, the isolate represents a new species of the genus Blastococcus, for which the name Blastococcuslitoris sp.
30215596	7	60	theme	characteristic	820:833	arg1	sugars					835:840	characteristic sugars	820:840	characteristic sugars	820:840	Whole-cell sugar analysis of strain GP-S2-8T revealed rhamnose, glucose and mannose as characteristic sugars.
30215596	2	61	theme	sediment	192:199	arg1	sample					201:206	a sea-tidal flat sediment sample	175:206	a sea-tidal flat sediment sample from Gopado, Republic of Korea	175:237	A novel Gram-strain-positive, non-spore-forming bacterial strain, designated GP-S2-8T, was isolated from a sea-tidal flat sediment sample from Gopado, Republic of Korea.
30215596	4	62	theme	w/v	506:508	arg1	NaCl					511:514	0-11 % (w/v) NaCl	498:514	0-11 % (w/v) NaCl (0-3 %)	498:522	The strain grew at 4-45 °C (optimum, 28-37 °C), at pH 4.0-11.0 (pH 7.0-9.0) and in the presence of 0-11 % (w/v) NaCl (0-3 %).
30215596	4	62	theme	w/v	506:508	arg1	%					521:521	0-3 %	517:521	0-3 %	517:521	The strain grew at 4-45 °C (optimum, 28-37 °C), at pH 4.0-11.0 (pH 7.0-9.0) and in the presence of 0-11 % (w/v) NaCl (0-3 %).
30215596	2	63	theme	flat	187:190	arg1	sample					201:206	a sea-tidal flat sediment sample	175:206	a sea-tidal flat sediment sample from Gopado, Republic of Korea	175:237	A novel Gram-strain-positive, non-spore-forming bacterial strain, designated GP-S2-8T, was isolated from a sea-tidal flat sediment sample from Gopado, Republic of Korea.
30215596	8	64	theme	respiratory	859:869	arg1	quinone					871:877	The predominant respiratory quinone	843:877	The predominant respiratory quinone	843:877	The predominant respiratory quinone was MK-9(H4) and the major fatty acids were iso-C16 : 0, iso-C16 : 1 H, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0 and iso-C14 : 0.
30215596	8	64	theme	respiratory	859:869	arg1	MK-9					883:886	MK-9	883:886	MK-9	883:886	The predominant respiratory quinone was MK-9(H4) and the major fatty acids were iso-C16 : 0, iso-C16 : 1 H, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0 and iso-C14 : 0.
30215596	11	65	theme	genus	1362:1366	arg1	Blastococcus					1368:1379	the genus Blastococcus	1358:1379	the genus Blastococcus	1358:1379	DNA-DNA relatedness values between strain GP-S2-8T and type strains of the genus Blastococcus ranged from 14.6 to 48.6 %.
30215596	12	66	theme	phenotypic	1429:1438	arg1	differences					1440:1450	the phenotypic differences	1425:1450	the phenotypic differences	1425:1450	On the basis of the phenotypic differences and DNA-DNA relatedness data, the isolate represents a new species of the genus Blastococcus, for which the name Blastococcuslitoris sp.
30215596	4	67	theme	NaCl	511:514	arg1	presence					486:493	the presence	482:493	the presence of 0-11 % (w/v) NaCl (0-3 %)	482:522	The strain grew at 4-45 °C (optimum, 28-37 °C), at pH 4.0-11.0 (pH 7.0-9.0) and in the presence of 0-11 % (w/v) NaCl (0-3 %).
31647399	11	0	theme	phenotypic	1510:1519	arg1	characteristics					1521:1535	phylogenetic, genomic, chemotaxonomic and phenotypic characteristics	1468:1535	phylogenetic, genomic, chemotaxonomic and phenotypic characteristics	1468:1535	Based on phylogenetic, genomic, chemotaxonomic and phenotypic characteristics, strain 7197T was classified as a novel species within the genus Paenibacillus, for which the name Paenibacillus rhizophilus sp.
31647399	11	1	theme	phylogenetic	1468:1479	arg1	characteristics					1521:1535	phylogenetic, genomic, chemotaxonomic and phenotypic characteristics	1468:1535	phylogenetic, genomic, chemotaxonomic and phenotypic characteristics	1468:1535	Based on phylogenetic, genomic, chemotaxonomic and phenotypic characteristics, strain 7197T was classified as a novel species within the genus Paenibacillus, for which the name Paenibacillus rhizophilus sp.
31647399	11	2	theme	strain	1538:1543	arg1	species					1577:1583	a novel species	1569:1583	a novel species within the genus Paenibacillus, for which the name Paenibacillus rhizophilus sp	1569:1663	Based on phylogenetic, genomic, chemotaxonomic and phenotypic characteristics, strain 7197T was classified as a novel species within the genus Paenibacillus, for which the name Paenibacillus rhizophilus sp.
31647399	11	2	theme	strain	1538:1543	arg1	7197T					1545:1549	strain 7197T	1538:1549	strain 7197T	1538:1549	Based on phylogenetic, genomic, chemotaxonomic and phenotypic characteristics, strain 7197T was classified as a novel species within the genus Paenibacillus, for which the name Paenibacillus rhizophilus sp.
31647399	9	3	theme	respiratory	1348:1358	arg1	quinone					1360:1366	the major respiratory quinone	1338:1366	the major respiratory quinone	1338:1366	Menaquinone-7 was reported as the major respiratory quinone.
31647399	9	3	theme	respiratory	1348:1358	arg1	Menaquinone-7					1308:1320	Menaquinone-7	1308:1320	Menaquinone-7	1308:1320	Menaquinone-7 was reported as the major respiratory quinone.
31647399	5	4	theme	average	699:705	arg1	values					727:732	The average nucleotide identity values	695:732	The average nucleotide identity values of genome sequences between the novel isolate and the type strains of related species P. sabinae T27T, P. sophorae S27T and P. forsythiae T98T	695:875	The average nucleotide identity values of genome sequences between the novel isolate and the type strains of related species P. sabinae T27T, P. sophorae S27T and P. forsythiae T98T were 87.9 %, 85.8 and 83.9 %, respectively.
31647399	5	4	theme	average	699:705	arg1	%					887:887	87.9 %	882:887	87.9 %	882:887	The average nucleotide identity values of genome sequences between the novel isolate and the type strains of related species P. sabinae T27T, P. sophorae S27T and P. forsythiae T98T were 87.9 %, 85.8 and 83.9 %, respectively.
31647399	11	5	theme	novel	1571:1575	arg1	species					1577:1583	a novel species	1569:1583	a novel species within the genus Paenibacillus, for which the name Paenibacillus rhizophilus sp	1569:1663	Based on phylogenetic, genomic, chemotaxonomic and phenotypic characteristics, strain 7197T was classified as a novel species within the genus Paenibacillus, for which the name Paenibacillus rhizophilus sp.
31647399	11	5	theme	novel	1571:1575	arg1	7197T					1545:1549	strain 7197T	1538:1549	strain 7197T	1538:1549	Based on phylogenetic, genomic, chemotaxonomic and phenotypic characteristics, strain 7197T was classified as a novel species within the genus Paenibacillus, for which the name Paenibacillus rhizophilus sp.
31647399	4	6	theme	strain	620:625	arg1	relatedness					600:610	the genomic relatedness	588:610	the genomic relatedness of this strain to its phylogenetic neighbours	588:656	To ascertain the genomic relatedness of this strain to its phylogenetic neighbours, its genome sequence was determined.
31647399	11	7	theme	Paenibacillus	1636:1648	arg1	sp					1662:1663	the name Paenibacillus rhizophilus sp	1627:1663	the name Paenibacillus rhizophilus sp	1627:1663	Based on phylogenetic, genomic, chemotaxonomic and phenotypic characteristics, strain 7197T was classified as a novel species within the genus Paenibacillus, for which the name Paenibacillus rhizophilus sp.
31647399	11	8	theme	name	1631:1634	arg1	sp					1662:1663	the name Paenibacillus rhizophilus sp	1627:1663	the name Paenibacillus rhizophilus sp	1627:1663	Based on phylogenetic, genomic, chemotaxonomic and phenotypic characteristics, strain 7197T was classified as a novel species within the genus Paenibacillus, for which the name Paenibacillus rhizophilus sp.
31647399	11	9	theme	chemotaxonomic	1491:1504	arg1	characteristics					1521:1535	phylogenetic, genomic, chemotaxonomic and phenotypic characteristics	1468:1535	phylogenetic, genomic, chemotaxonomic and phenotypic characteristics	1468:1535	Based on phylogenetic, genomic, chemotaxonomic and phenotypic characteristics, strain 7197T was classified as a novel species within the genus Paenibacillus, for which the name Paenibacillus rhizophilus sp.
31647399	10	10	from	acid	1381:1384	arg1	peptidoglycan					1403:1415	the cell-wall peptidoglycan	1389:1415	the cell-wall peptidoglycan	1389:1415	The diamino acid in the cell-wall peptidoglycan was found to be meso-diaminopimelic acid.
31647399	2	11	theme	novel	129:133	arg1	bacterium					215:223	endospore-forming, motile, rod-shaped, facultative aerobic bacterium	156:223	bacterium	215:223	A novel Gram-stain-variable, endospore-forming, motile, rod-shaped, facultative aerobic bacterium, designated 7197T, was isolated from rhizosphere soil of wheat (Triticum aestivum L.) collected from Yakeshi County, Inner Mongolia, PR China.
31647399	2	11	theme	novel	129:133	arg1	Gram-stain-variable					135:153	A novel Gram-stain-variable	127:153	A novel Gram-stain-variable	127:153	A novel Gram-stain-variable, endospore-forming, motile, rod-shaped, facultative aerobic bacterium, designated 7197T, was isolated from rhizosphere soil of wheat (Triticum aestivum L.) collected from Yakeshi County, Inner Mongolia, PR China.
31647399	8	12	theme	genome	1198:1203	arg1	5.21 Mb					1230:1236	5.21 Mb	1230:1236	5.21 Mb	1230:1236	The genome size of strain 7197T was 5.21 Mb, comprising 4879 predicted genes with a DNA G+C content of 51.9 mol%.
31647399	8	12	theme	genome	1198:1203	arg1	size					1205:1208	The genome size	1194:1208	The genome size of strain 7197T	1194:1224	The genome size of strain 7197T was 5.21 Mb, comprising 4879 predicted genes with a DNA G+C content of 51.9 mol%.
31647399	2	13	theme	rhizosphere	262:272	arg1	soil					274:277	rhizosphere soil	262:277	rhizosphere soil of wheat (Triticum aestivum L.) collected from Yakeshi County, Inner Mongolia, PR China	262:365	A novel Gram-stain-variable, endospore-forming, motile, rod-shaped, facultative aerobic bacterium, designated 7197T, was isolated from rhizosphere soil of wheat (Triticum aestivum L.) collected from Yakeshi County, Inner Mongolia, PR China.
31647399	13	14	theme	rhizophilus	1717:1727	arg1	7197T					1732:1736	7197T	1732:1736	7197T (=DSM 103168T=CGMCC 1.15699T)	1732:1766	The type strain of Paenibacillus rhizophilus is 7197T (=DSM 103168T=CGMCC 1.15699T).
31647399	13	14	theme	rhizophilus	1717:1727	arg1	strain					1693:1698	The type strain	1684:1698	The type strain of Paenibacillus rhizophilus	1684:1727	The type strain of Paenibacillus rhizophilus is 7197T (=DSM 103168T=CGMCC 1.15699T).
31647399	2	15	dep	endospore-forming	156:172	arg1	rod-shaped					183:192	rod-shaped	183:192	rod-shaped	183:192	A novel Gram-stain-variable, endospore-forming, motile, rod-shaped, facultative aerobic bacterium, designated 7197T, was isolated from rhizosphere soil of wheat (Triticum aestivum L.) collected from Yakeshi County, Inner Mongolia, PR China.
31647399	2	15	dep	endospore-forming	156:172	arg1	motile					175:180	motile	175:180	motile	175:180	A novel Gram-stain-variable, endospore-forming, motile, rod-shaped, facultative aerobic bacterium, designated 7197T, was isolated from rhizosphere soil of wheat (Triticum aestivum L.) collected from Yakeshi County, Inner Mongolia, PR China.
31647399	5	16	dep	strains	793:799	arg1	P.					837:838	P.	837:838	P.	837:838	The average nucleotide identity values of genome sequences between the novel isolate and the type strains of related species P. sabinae T27T, P. sophorae S27T and P. forsythiae T98T were 87.9 %, 85.8 and 83.9 %, respectively.
31647399	5	16	dep	strains	793:799	arg1	T98T					872:875	T98T	872:875	T98T	872:875	The average nucleotide identity values of genome sequences between the novel isolate and the type strains of related species P. sabinae T27T, P. sophorae S27T and P. forsythiae T98T were 87.9 %, 85.8 and 83.9 %, respectively.
31647399	5	16	dep	strains	793:799	arg1	strains					793:799	the type strains	784:799	the type strains of related species P. sabinae T27T, P. sophorae S27T and P. forsythiae T98T	784:875	The average nucleotide identity values of genome sequences between the novel isolate and the type strains of related species P. sabinae T27T, P. sophorae S27T and P. forsythiae T98T were 87.9 %, 85.8 and 83.9 %, respectively.
31647399	5	16	dep	strains	793:799	arg1	S27T					849:852	S27T	849:852	S27T	849:852	The average nucleotide identity values of genome sequences between the novel isolate and the type strains of related species P. sabinae T27T, P. sophorae S27T and P. forsythiae T98T were 87.9 %, 85.8 and 83.9 %, respectively.
31647399	8	17	theme	predicted	1255:1263	arg1	genes					1265:1269	4879 predicted genes	1250:1269	4879 predicted genes	1250:1269	The genome size of strain 7197T was 5.21 Mb, comprising 4879 predicted genes with a DNA G+C content of 51.9 mol%.
31647399	13	18	theme	103168T=CGMCC	1744:1756	arg1	1.15699T					1758:1765	=DSM 103168T=CGMCC 1.15699T	1739:1765	=DSM 103168T=CGMCC 1.15699T	1739:1765	The type strain of Paenibacillus rhizophilus is 7197T (=DSM 103168T=CGMCC 1.15699T).
31647399	13	18	theme	103168T=CGMCC	1744:1756	arg1	7197T					1732:1736	7197T	1732:1736	7197T (=DSM 103168T=CGMCC 1.15699T)	1732:1766	The type strain of Paenibacillus rhizophilus is 7197T (=DSM 103168T=CGMCC 1.15699T).
31647399	1	19	dep	Triticum	104:111	arg1	L					122:122	L	122:122	Triticum aestivum L.	104:123	nov., a nitrogen-fixing bacterium isolated from the rhizosphere of wheat (Triticum aestivum L.).
31647399	1	19	dep	Triticum	104:111	arg1	aestivum					113:120	Triticum aestivum L.	104:123	Triticum aestivum L.	104:123	nov., a nitrogen-fixing bacterium isolated from the rhizosphere of wheat (Triticum aestivum L.).
31647399	5	20	theme	type	788:791	arg1	strains					793:799	the type strains	784:799	the type strains of related species P. sabinae T27T, P. sophorae S27T and P. forsythiae T98T	784:875	The average nucleotide identity values of genome sequences between the novel isolate and the type strains of related species P. sabinae T27T, P. sophorae S27T and P. forsythiae T98T were 87.9 %, 85.8 and 83.9 %, respectively.
31647399	5	20	theme	type	788:791	arg1	T98T					872:875	T98T	872:875	T98T	872:875	The average nucleotide identity values of genome sequences between the novel isolate and the type strains of related species P. sabinae T27T, P. sophorae S27T and P. forsythiae T98T were 87.9 %, 85.8 and 83.9 %, respectively.
31647399	5	20	theme	type	788:791	arg1	S27T					849:852	S27T	849:852	S27T	849:852	The average nucleotide identity values of genome sequences between the novel isolate and the type strains of related species P. sabinae T27T, P. sophorae S27T and P. forsythiae T98T were 87.9 %, 85.8 and 83.9 %, respectively.
31647399	6	21	contain	contained	938:946	arg2	phosphatidylglycerol					972:991	phosphatidylglycerol	972:991	phosphatidylglycerol	972:991	The polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, four unidentified aminophospholipids and one unidentified aminolipid.
31647399	6	21	contain	contained	938:946	arg2	aminophospholipids					1038:1055	four unidentified aminophospholipids	1020:1055	four unidentified aminophospholipids	1020:1055	The polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, four unidentified aminophospholipids and one unidentified aminolipid.
31647399	6	21	contain	contained	938:946	arg1	lipids					931:936	The polar lipids	921:936	The polar lipids	921:936	The polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, four unidentified aminophospholipids and one unidentified aminolipid.
31647399	6	21	contain	contained	938:946	arg2	phosphatidylethanolamine					994:1017	phosphatidylethanolamine	994:1017	phosphatidylethanolamine	994:1017	The polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, four unidentified aminophospholipids and one unidentified aminolipid.
31647399	6	21	contain	contained	938:946	arg2	aminolipid					1078:1087	one unidentified aminolipid	1061:1087	one unidentified aminolipid	1061:1087	The polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, four unidentified aminophospholipids and one unidentified aminolipid.
31647399	6	21	contain	contained	938:946	arg2	diphosphatidylglycerol					948:969	diphosphatidylglycerol	948:969	diphosphatidylglycerol	948:969	The polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, four unidentified aminophospholipids and one unidentified aminolipid.
31647399	7	22	theme	major	1094:1098	arg1	acids					1115:1119	The major cellular fatty acids	1090:1119	The major cellular fatty acids	1090:1119	The major cellular fatty acids were anteiso-C15 : 0 (56.3 %), C16 : 0 (15.7 %) and iso-C15 : 0 (14.1 %).
31647399	7	22	theme	major	1094:1098	arg1	anteiso-C15 					1126:1137	anteiso-C15 	1126:1137	anteiso-C15 	1126:1137	The major cellular fatty acids were anteiso-C15 : 0 (56.3 %), C16 : 0 (15.7 %) and iso-C15 : 0 (14.1 %).
31647399	5	23	theme	sequences	744:752	arg1	values					727:732	The average nucleotide identity values	695:732	The average nucleotide identity values of genome sequences between the novel isolate and the type strains of related species P. sabinae T27T, P. sophorae S27T and P. forsythiae T98T	695:875	The average nucleotide identity values of genome sequences between the novel isolate and the type strains of related species P. sabinae T27T, P. sophorae S27T and P. forsythiae T98T were 87.9 %, 85.8 and 83.9 %, respectively.
31647399	5	23	theme	sequences	744:752	arg1	%					887:887	87.9 %	882:887	87.9 %	882:887	The average nucleotide identity values of genome sequences between the novel isolate and the type strains of related species P. sabinae T27T, P. sophorae S27T and P. forsythiae T98T were 87.9 %, 85.8 and 83.9 %, respectively.
31647399	2	24	dep	Triticum	289:296	arg1	L.					307:308	Triticum aestivum L.	289:308	Triticum aestivum L.	289:308	A novel Gram-stain-variable, endospore-forming, motile, rod-shaped, facultative aerobic bacterium, designated 7197T, was isolated from rhizosphere soil of wheat (Triticum aestivum L.) collected from Yakeshi County, Inner Mongolia, PR China.
31647399	0	25	theme	rhizophilus	14:24	arg1	sp					26:27	Paenibacillus rhizophilus sp	0:27	Paenibacillus rhizophilus sp.	0:28	Paenibacillus rhizophilus sp.
31647399	11	26	theme	genomic	1482:1488	arg1	characteristics					1521:1535	phylogenetic, genomic, chemotaxonomic and phenotypic characteristics	1468:1535	phylogenetic, genomic, chemotaxonomic and phenotypic characteristics	1468:1535	Based on phylogenetic, genomic, chemotaxonomic and phenotypic characteristics, strain 7197T was classified as a novel species within the genus Paenibacillus, for which the name Paenibacillus rhizophilus sp.
31647399	4	27	theme	genome	663:668	arg1	sequence					670:677	its genome sequence	659:677	its genome sequence	659:677	To ascertain the genomic relatedness of this strain to its phylogenetic neighbours, its genome sequence was determined.
31647399	0	28	theme	Paenibacillus	0:12	arg1	sp					26:27	Paenibacillus rhizophilus sp	0:27	Paenibacillus rhizophilus sp.	0:28	Paenibacillus rhizophilus sp.
31647399	1	29	attach	isolated	64:71	arg2	bacterium					54:62	a nitrogen-fixing bacterium	36:62	a nitrogen-fixing bacterium isolated from the rhizosphere of wheat (Triticum aestivum L.)	36:124	nov., a nitrogen-fixing bacterium isolated from the rhizosphere of wheat (Triticum aestivum L.).
31647399	1	29	attach	isolated	64:71	arg1	rhizosphere					82:92	the rhizosphere	78:92	the rhizosphere of wheat (Triticum aestivum L.)	78:124	nov., a nitrogen-fixing bacterium isolated from the rhizosphere of wheat (Triticum aestivum L.).
31647399	8	30	theme	7197T	1220:1224	arg1	5.21 Mb					1230:1236	5.21 Mb	1230:1236	5.21 Mb	1230:1236	The genome size of strain 7197T was 5.21 Mb, comprising 4879 predicted genes with a DNA G+C content of 51.9 mol%.
31647399	8	30	theme	7197T	1220:1224	arg1	size					1205:1208	The genome size	1194:1208	The genome size of strain 7197T	1194:1224	The genome size of strain 7197T was 5.21 Mb, comprising 4879 predicted genes with a DNA G+C content of 51.9 mol%.
31647399	13	31	theme	type	1688:1691	arg1	7197T					1732:1736	7197T	1732:1736	7197T (=DSM 103168T=CGMCC 1.15699T)	1732:1766	The type strain of Paenibacillus rhizophilus is 7197T (=DSM 103168T=CGMCC 1.15699T).
31647399	13	31	theme	type	1688:1691	arg1	strain					1693:1698	The type strain	1684:1698	The type strain of Paenibacillus rhizophilus	1684:1727	The type strain of Paenibacillus rhizophilus is 7197T (=DSM 103168T=CGMCC 1.15699T).
31647399	2	32	theme	Inner	342:346	arg1	County					334:339	County	334:339	County	334:339	A novel Gram-stain-variable, endospore-forming, motile, rod-shaped, facultative aerobic bacterium, designated 7197T, was isolated from rhizosphere soil of wheat (Triticum aestivum L.) collected from Yakeshi County, Inner Mongolia, PR China.
31647399	2	32	theme	Inner	342:346	arg1	Mongolia					348:355	Inner Mongolia	342:355	Inner Mongolia	342:355	A novel Gram-stain-variable, endospore-forming, motile, rod-shaped, facultative aerobic bacterium, designated 7197T, was isolated from rhizosphere soil of wheat (Triticum aestivum L.) collected from Yakeshi County, Inner Mongolia, PR China.
31647399	7	33	theme	fatty	1109:1113	arg1	acids					1115:1119	The major cellular fatty acids	1090:1119	The major cellular fatty acids	1090:1119	The major cellular fatty acids were anteiso-C15 : 0 (56.3 %), C16 : 0 (15.7 %) and iso-C15 : 0 (14.1 %).
31647399	7	33	theme	fatty	1109:1113	arg1	anteiso-C15 					1126:1137	anteiso-C15 	1126:1137	anteiso-C15 	1126:1137	The major cellular fatty acids were anteiso-C15 : 0 (56.3 %), C16 : 0 (15.7 %) and iso-C15 : 0 (14.1 %).
31647399	3	34	dep	have	394:397	arg1	%					480:480	98.0 %	475:480	98.0 %	475:480	This isolate was found to have the highest 16S rRNA gene sequence similarity to Paenibacillussabinae T27T (98.0 %), followed by Paenibacillussophorae S27T (97.9 %) and Paenibacillusforsythiae T98T (97.7 %).
31647399	2	35	theme	wheat	282:286	arg1	soil					274:277	rhizosphere soil	262:277	rhizosphere soil of wheat (Triticum aestivum L.) collected from Yakeshi County, Inner Mongolia, PR China	262:365	A novel Gram-stain-variable, endospore-forming, motile, rod-shaped, facultative aerobic bacterium, designated 7197T, was isolated from rhizosphere soil of wheat (Triticum aestivum L.) collected from Yakeshi County, Inner Mongolia, PR China.
31647399	3	36	theme	16S	411:413	arg1	similarity					434:443	the highest 16S rRNA gene sequence similarity	399:443	the highest 16S rRNA gene sequence similarity to Paenibacillussabinae T27T	399:472	This isolate was found to have the highest 16S rRNA gene sequence similarity to Paenibacillussabinae T27T (98.0 %), followed by Paenibacillussophorae S27T (97.9 %) and Paenibacillusforsythiae T98T (97.7 %).
31647399	10	37	theme	meso-diaminopimelic	1433:1451	arg1	acid					1453:1456	meso-diaminopimelic acid	1433:1456	meso-diaminopimelic acid	1433:1456	The diamino acid in the cell-wall peptidoglycan was found to be meso-diaminopimelic acid.
31647399	10	37	theme	meso-diaminopimelic	1433:1451	arg1	acid					1381:1384	The diamino acid	1369:1384	The diamino acid in the cell-wall peptidoglycan	1369:1415	The diamino acid in the cell-wall peptidoglycan was found to be meso-diaminopimelic acid.
31647399	11	38	theme	rhizophilus	1650:1660	arg1	sp					1662:1663	the name Paenibacillus rhizophilus sp	1627:1663	the name Paenibacillus rhizophilus sp	1627:1663	Based on phylogenetic, genomic, chemotaxonomic and phenotypic characteristics, strain 7197T was classified as a novel species within the genus Paenibacillus, for which the name Paenibacillus rhizophilus sp.
31647399	8	39	theme	strain	1213:1218	arg1	7197T					1220:1224	strain 7197T	1213:1224	strain 7197T	1213:1224	The genome size of strain 7197T was 5.21 Mb, comprising 4879 predicted genes with a DNA G+C content of 51.9 mol%.
31647399	8	40	theme	51.9 mol	1297:1304	arg1	%					1305:1305	51.9 mol%	1297:1305	51.9 mol%	1297:1305	The genome size of strain 7197T was 5.21 Mb, comprising 4879 predicted genes with a DNA G+C content of 51.9 mol%.
31647399	13	41	theme	Paenibacillus	1703:1715	arg1	rhizophilus					1717:1727	Paenibacillus rhizophilus	1703:1727	Paenibacillus rhizophilus	1703:1727	The type strain of Paenibacillus rhizophilus is 7197T (=DSM 103168T=CGMCC 1.15699T).
31647399	3	42	theme	rRNA	415:418	arg1	similarity					434:443	the highest 16S rRNA gene sequence similarity	399:443	the highest 16S rRNA gene sequence similarity to Paenibacillussabinae T27T	399:472	This isolate was found to have the highest 16S rRNA gene sequence similarity to Paenibacillussabinae T27T (98.0 %), followed by Paenibacillussophorae S27T (97.9 %) and Paenibacillusforsythiae T98T (97.7 %).
31647399	5	43	theme	nucleotide	707:716	arg1	values					727:732	The average nucleotide identity values	695:732	The average nucleotide identity values of genome sequences between the novel isolate and the type strains of related species P. sabinae T27T, P. sophorae S27T and P. forsythiae T98T	695:875	The average nucleotide identity values of genome sequences between the novel isolate and the type strains of related species P. sabinae T27T, P. sophorae S27T and P. forsythiae T98T were 87.9 %, 85.8 and 83.9 %, respectively.
31647399	5	43	theme	nucleotide	707:716	arg1	%					887:887	87.9 %	882:887	87.9 %	882:887	The average nucleotide identity values of genome sequences between the novel isolate and the type strains of related species P. sabinae T27T, P. sophorae S27T and P. forsythiae T98T were 87.9 %, 85.8 and 83.9 %, respectively.
31647399	9	44	theme	major	1342:1346	arg1	quinone					1360:1366	the major respiratory quinone	1338:1366	the major respiratory quinone	1338:1366	Menaquinone-7 was reported as the major respiratory quinone.
31647399	9	44	theme	major	1342:1346	arg1	Menaquinone-7					1308:1320	Menaquinone-7	1308:1320	Menaquinone-7	1308:1320	Menaquinone-7 was reported as the major respiratory quinone.
31647399	2	45	theme	endospore-forming	156:172	arg1	bacterium					215:223	endospore-forming, motile, rod-shaped, facultative aerobic bacterium	156:223	bacterium	215:223	A novel Gram-stain-variable, endospore-forming, motile, rod-shaped, facultative aerobic bacterium, designated 7197T, was isolated from rhizosphere soil of wheat (Triticum aestivum L.) collected from Yakeshi County, Inner Mongolia, PR China.
31647399	2	45	theme	endospore-forming	156:172	arg1	Gram-stain-variable					135:153	A novel Gram-stain-variable	127:153	A novel Gram-stain-variable	127:153	A novel Gram-stain-variable, endospore-forming, motile, rod-shaped, facultative aerobic bacterium, designated 7197T, was isolated from rhizosphere soil of wheat (Triticum aestivum L.) collected from Yakeshi County, Inner Mongolia, PR China.
31647399	4	46	theme	genomic	592:598	arg1	relatedness					600:610	the genomic relatedness	588:610	the genomic relatedness of this strain to its phylogenetic neighbours	588:656	To ascertain the genomic relatedness of this strain to its phylogenetic neighbours, its genome sequence was determined.
31647399	5	47	theme	identity	718:725	arg1	values					727:732	The average nucleotide identity values	695:732	The average nucleotide identity values of genome sequences between the novel isolate and the type strains of related species P. sabinae T27T, P. sophorae S27T and P. forsythiae T98T	695:875	The average nucleotide identity values of genome sequences between the novel isolate and the type strains of related species P. sabinae T27T, P. sophorae S27T and P. forsythiae T98T were 87.9 %, 85.8 and 83.9 %, respectively.
31647399	5	47	theme	identity	718:725	arg1	%					887:887	87.9 %	882:887	87.9 %	882:887	The average nucleotide identity values of genome sequences between the novel isolate and the type strains of related species P. sabinae T27T, P. sophorae S27T and P. forsythiae T98T were 87.9 %, 85.8 and 83.9 %, respectively.
31647399	7	48	theme	cellular	1100:1107	arg1	acids					1115:1119	The major cellular fatty acids	1090:1119	The major cellular fatty acids	1090:1119	The major cellular fatty acids were anteiso-C15 : 0 (56.3 %), C16 : 0 (15.7 %) and iso-C15 : 0 (14.1 %).
31647399	7	48	theme	cellular	1100:1107	arg1	anteiso-C15 					1126:1137	anteiso-C15 	1126:1137	anteiso-C15 	1126:1137	The major cellular fatty acids were anteiso-C15 : 0 (56.3 %), C16 : 0 (15.7 %) and iso-C15 : 0 (14.1 %).
31647399	3	49	theme	highest	403:409	arg1	similarity					434:443	the highest 16S rRNA gene sequence similarity	399:443	the highest 16S rRNA gene sequence similarity to Paenibacillussabinae T27T	399:472	This isolate was found to have the highest 16S rRNA gene sequence similarity to Paenibacillussabinae T27T (98.0 %), followed by Paenibacillussophorae S27T (97.9 %) and Paenibacillusforsythiae T98T (97.7 %).
31647399	6	50	theme	polar	925:929	arg1	lipids					931:936	The polar lipids	921:936	The polar lipids	921:936	The polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, four unidentified aminophospholipids and one unidentified aminolipid.
31647399	8	51	theme	%	1305:1305	arg1	G+C content					1282:1292	a DNA G+C content	1276:1292	a DNA G+C content of 51.9 mol%	1276:1305	The genome size of strain 7197T was 5.21 Mb, comprising 4879 predicted genes with a DNA G+C content of 51.9 mol%.
31647399	2	52	theme	aerobic	207:213	arg1	bacterium					215:223	endospore-forming, motile, rod-shaped, facultative aerobic bacterium	156:223	bacterium	215:223	A novel Gram-stain-variable, endospore-forming, motile, rod-shaped, facultative aerobic bacterium, designated 7197T, was isolated from rhizosphere soil of wheat (Triticum aestivum L.) collected from Yakeshi County, Inner Mongolia, PR China.
31647399	2	52	theme	aerobic	207:213	arg1	Gram-stain-variable					135:153	A novel Gram-stain-variable	127:153	A novel Gram-stain-variable	127:153	A novel Gram-stain-variable, endospore-forming, motile, rod-shaped, facultative aerobic bacterium, designated 7197T, was isolated from rhizosphere soil of wheat (Triticum aestivum L.) collected from Yakeshi County, Inner Mongolia, PR China.
31647399	4	53	theme	phylogenetic	634:645	arg1	neighbours					647:656	its phylogenetic neighbours	630:656	its phylogenetic neighbours	630:656	To ascertain the genomic relatedness of this strain to its phylogenetic neighbours, its genome sequence was determined.
31647399	2	54	attach	isolated	248:255	arg2	Gram-stain-variable					135:153	A novel Gram-stain-variable	127:153	A novel Gram-stain-variable	127:153	A novel Gram-stain-variable, endospore-forming, motile, rod-shaped, facultative aerobic bacterium, designated 7197T, was isolated from rhizosphere soil of wheat (Triticum aestivum L.) collected from Yakeshi County, Inner Mongolia, PR China.
31647399	2	54	attach	isolated	248:255	arg1	soil					274:277	rhizosphere soil	262:277	rhizosphere soil of wheat (Triticum aestivum L.) collected from Yakeshi County, Inner Mongolia, PR China	262:365	A novel Gram-stain-variable, endospore-forming, motile, rod-shaped, facultative aerobic bacterium, designated 7197T, was isolated from rhizosphere soil of wheat (Triticum aestivum L.) collected from Yakeshi County, Inner Mongolia, PR China.
31647399	2	54	attach	isolated	248:255	arg2	bacterium					215:223	endospore-forming, motile, rod-shaped, facultative aerobic bacterium	156:223	bacterium	215:223	A novel Gram-stain-variable, endospore-forming, motile, rod-shaped, facultative aerobic bacterium, designated 7197T, was isolated from rhizosphere soil of wheat (Triticum aestivum L.) collected from Yakeshi County, Inner Mongolia, PR China.
31647399	10	55	theme	diamino	1373:1379	arg1	acid					1453:1456	meso-diaminopimelic acid	1433:1456	meso-diaminopimelic acid	1433:1456	The diamino acid in the cell-wall peptidoglycan was found to be meso-diaminopimelic acid.
31647399	10	55	theme	diamino	1373:1379	arg1	acid					1381:1384	The diamino acid	1369:1384	The diamino acid in the cell-wall peptidoglycan	1369:1415	The diamino acid in the cell-wall peptidoglycan was found to be meso-diaminopimelic acid.
31647399	2	56	theme	facultative	195:205	arg1	bacterium					215:223	endospore-forming, motile, rod-shaped, facultative aerobic bacterium	156:223	bacterium	215:223	A novel Gram-stain-variable, endospore-forming, motile, rod-shaped, facultative aerobic bacterium, designated 7197T, was isolated from rhizosphere soil of wheat (Triticum aestivum L.) collected from Yakeshi County, Inner Mongolia, PR China.
31647399	2	56	theme	facultative	195:205	arg1	Gram-stain-variable					135:153	A novel Gram-stain-variable	127:153	A novel Gram-stain-variable	127:153	A novel Gram-stain-variable, endospore-forming, motile, rod-shaped, facultative aerobic bacterium, designated 7197T, was isolated from rhizosphere soil of wheat (Triticum aestivum L.) collected from Yakeshi County, Inner Mongolia, PR China.
31647399	6	57	theme	unidentified	1065:1076	arg1	aminolipid					1078:1087	one unidentified aminolipid	1061:1087	one unidentified aminolipid	1061:1087	The polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, four unidentified aminophospholipids and one unidentified aminolipid.
31647399	8	58	theme	DNA	1278:1280	arg1	G+C content					1282:1292	a DNA G+C content	1276:1292	a DNA G+C content of 51.9 mol%	1276:1305	The genome size of strain 7197T was 5.21 Mb, comprising 4879 predicted genes with a DNA G+C content of 51.9 mol%.
31647399	5	59	theme	related	804:810	arg1	species					812:818	related species	804:818	related species	804:818	The average nucleotide identity values of genome sequences between the novel isolate and the type strains of related species P. sabinae T27T, P. sophorae S27T and P. forsythiae T98T were 87.9 %, 85.8 and 83.9 %, respectively.
31647399	1	60	dep	bacterium	54:62	arg1	nov.					30:33	nov.	30:33	nov.	30:33	nov., a nitrogen-fixing bacterium isolated from the rhizosphere of wheat (Triticum aestivum L.).
31647399	1	61	theme	wheat	97:101	arg1	rhizosphere					82:92	the rhizosphere	78:92	the rhizosphere of wheat (Triticum aestivum L.)	78:124	nov., a nitrogen-fixing bacterium isolated from the rhizosphere of wheat (Triticum aestivum L.).
31647399	3	62	contain	have	394:397	arg2	similarity					434:443	the highest 16S rRNA gene sequence similarity	399:443	the highest 16S rRNA gene sequence similarity to Paenibacillussabinae T27T	399:472	This isolate was found to have the highest 16S rRNA gene sequence similarity to Paenibacillussabinae T27T (98.0 %), followed by Paenibacillussophorae S27T (97.9 %) and Paenibacillusforsythiae T98T (97.7 %).
31647399	3	62	contain	have	394:397	arg1	isolate					373:379	This isolate	368:379	This isolate	368:379	This isolate was found to have the highest 16S rRNA gene sequence similarity to Paenibacillussabinae T27T (98.0 %), followed by Paenibacillussophorae S27T (97.9 %) and Paenibacillusforsythiae T98T (97.7 %).
31647399	3	63	theme	sequence	425:432	arg1	similarity					434:443	the highest 16S rRNA gene sequence similarity	399:443	the highest 16S rRNA gene sequence similarity to Paenibacillussabinae T27T	399:472	This isolate was found to have the highest 16S rRNA gene sequence similarity to Paenibacillussabinae T27T (98.0 %), followed by Paenibacillussophorae S27T (97.9 %) and Paenibacillusforsythiae T98T (97.7 %).
31647399	3	64	theme	gene	420:423	arg1	similarity					434:443	the highest 16S rRNA gene sequence similarity	399:443	the highest 16S rRNA gene sequence similarity to Paenibacillussabinae T27T	399:472	This isolate was found to have the highest 16S rRNA gene sequence similarity to Paenibacillussabinae T27T (98.0 %), followed by Paenibacillussophorae S27T (97.9 %) and Paenibacillusforsythiae T98T (97.7 %).
31647399	6	65	theme	unidentified	1025:1036	arg1	aminophospholipids					1038:1055	four unidentified aminophospholipids	1020:1055	four unidentified aminophospholipids	1020:1055	The polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, four unidentified aminophospholipids and one unidentified aminolipid.
31647399	5	66	theme	novel	766:770	arg1	isolate					772:778	the novel isolate	762:778	the novel isolate	762:778	The average nucleotide identity values of genome sequences between the novel isolate and the type strains of related species P. sabinae T27T, P. sophorae S27T and P. forsythiae T98T were 87.9 %, 85.8 and 83.9 %, respectively.
31647399	5	67	theme	species	812:818	arg1	strains					793:799	the type strains	784:799	the type strains of related species P. sabinae T27T, P. sophorae S27T and P. forsythiae T98T	784:875	The average nucleotide identity values of genome sequences between the novel isolate and the type strains of related species P. sabinae T27T, P. sophorae S27T and P. forsythiae T98T were 87.9 %, 85.8 and 83.9 %, respectively.
31647399	5	67	theme	species	812:818	arg1	T98T					872:875	T98T	872:875	T98T	872:875	The average nucleotide identity values of genome sequences between the novel isolate and the type strains of related species P. sabinae T27T, P. sophorae S27T and P. forsythiae T98T were 87.9 %, 85.8 and 83.9 %, respectively.
31647399	5	67	theme	species	812:818	arg1	isolate					772:778	the novel isolate	762:778	the novel isolate	762:778	The average nucleotide identity values of genome sequences between the novel isolate and the type strains of related species P. sabinae T27T, P. sophorae S27T and P. forsythiae T98T were 87.9 %, 85.8 and 83.9 %, respectively.
31647399	5	67	theme	species	812:818	arg1	S27T					849:852	S27T	849:852	S27T	849:852	The average nucleotide identity values of genome sequences between the novel isolate and the type strains of related species P. sabinae T27T, P. sophorae S27T and P. forsythiae T98T were 87.9 %, 85.8 and 83.9 %, respectively.
31647399	10	68	theme	cell-wall	1393:1401	arg1	peptidoglycan					1403:1415	the cell-wall peptidoglycan	1389:1415	the cell-wall peptidoglycan	1389:1415	The diamino acid in the cell-wall peptidoglycan was found to be meso-diaminopimelic acid.
31647399	1	69	theme	nitrogen-fixing	38:52	arg1	bacterium					54:62	a nitrogen-fixing bacterium	36:62	a nitrogen-fixing bacterium isolated from the rhizosphere of wheat (Triticum aestivum L.)	36:124	nov., a nitrogen-fixing bacterium isolated from the rhizosphere of wheat (Triticum aestivum L.).
31647399	5	70	theme	genome	737:742	arg1	sequences					744:752	genome sequences	737:752	genome sequences	737:752	The average nucleotide identity values of genome sequences between the novel isolate and the type strains of related species P. sabinae T27T, P. sophorae S27T and P. forsythiae T98T were 87.9 %, 85.8 and 83.9 %, respectively.
31647399	13	71	theme	=DSM	1739:1742	arg1	1.15699T					1758:1765	=DSM 103168T=CGMCC 1.15699T	1739:1765	=DSM 103168T=CGMCC 1.15699T	1739:1765	The type strain of Paenibacillus rhizophilus is 7197T (=DSM 103168T=CGMCC 1.15699T).
31647399	13	71	theme	=DSM	1739:1742	arg1	7197T					1732:1736	7197T	1732:1736	7197T (=DSM 103168T=CGMCC 1.15699T)	1732:1766	The type strain of Paenibacillus rhizophilus is 7197T (=DSM 103168T=CGMCC 1.15699T).
31647399	11	72	theme	Paenibacillus	1602:1614	arg1	genus					1596:1600	the genus Paenibacillus	1592:1614	the genus Paenibacillus	1592:1614	Based on phylogenetic, genomic, chemotaxonomic and phenotypic characteristics, strain 7197T was classified as a novel species within the genus Paenibacillus, for which the name Paenibacillus rhizophilus sp.
30308045	8	0	theme	intestinal	1811:1820	arg1	diversity					1831:1839	diversity	1831:1839	diversity	1831:1839	Overall, altering dietary iron intake exacerbated DSS-induced colitis; increasing the iron content of the diet also led to changes in intestinal bacteria diversity and composition after colitis was induced with DSS.
30308045	8	0	theme	intestinal	1811:1820	arg1	bacteria					1822:1829	intestinal bacteria diversity and composition	1811:1855	intestinal bacteria diversity and composition	1811:1855	Overall, altering dietary iron intake exacerbated DSS-induced colitis; increasing the iron content of the diet also led to changes in intestinal bacteria diversity and composition after colitis was induced with DSS.
30308045	8	0	theme	intestinal	1811:1820	arg1	composition					1845:1855	composition	1845:1855	composition	1845:1855	Overall, altering dietary iron intake exacerbated DSS-induced colitis; increasing the iron content of the diet also led to changes in intestinal bacteria diversity and composition after colitis was induced with DSS.
30308045	4	1	theme	DSS-induced	927:937	arg1	colitis					939:945	DSS-induced colitis	927:945	DSS-induced colitis	927:945	Increasing or decreasing dietary iron concentration from the standard 200 ppm exacerbated both clinical and histological severity of DSS-induced colitis.
30308045	3	2	theme	sulfate	610:616	arg1	DSS					626:628	DSS	626:628	DSS	626:628	Here, we assessed the impact of dietary iron, using chow diets containing either 100, 200 or 400 ppm, fed ad libitum to adult female C57BL/6 mice in the presence or absence of colitis induced using dextran sulfate sodium (DSS), on (i) clinical and histological severity of acute DSS-induced colitis, and (ii) faecal microbial diversity, as assessed by sequencing the V4 region of 16S rRNA.
30308045	3	2	theme	sulfate	610:616	arg1	sodium					618:623	dextran sulfate sodium	602:623	dextran sulfate sodium (DSS)	602:629	Here, we assessed the impact of dietary iron, using chow diets containing either 100, 200 or 400 ppm, fed ad libitum to adult female C57BL/6 mice in the presence or absence of colitis induced using dextran sulfate sodium (DSS), on (i) clinical and histological severity of acute DSS-induced colitis, and (ii) faecal microbial diversity, as assessed by sequencing the V4 region of 16S rRNA.
30308045	2	3	theme	iron	319:322	arg1	supplementation					324:338	iron supplementation	319:338	iron supplementation	319:338	The intestinal microbiota also plays a key role in the pathogenesis of IBD, and iron supplementation likely influences gut bacterial diversity in patients with IBD.
30308045	5	4	theme	body	1069:1072	arg1	weight					1074:1079	more body weight	1064:1079	more body weight than those receiving double the amount of standard iron (i.e. 400 ppm); p<0.01	1064:1158	DSS-treated mice provided only half the standard levels of iron ad libitum (i.e. chow containing 100 ppm iron) lost more body weight than those receiving double the amount of standard iron (i.e. 400 ppm); p<0.01.
30308045	5	5	dep	ppm	1147:1149	arg1	i.e.					1138:1141	i.e.	1138:1141	i.e.	1138:1141	DSS-treated mice provided only half the standard levels of iron ad libitum (i.e. chow containing 100 ppm iron) lost more body weight than those receiving double the amount of standard iron (i.e. 400 ppm); p<0.01.
30308045	2	6	with	patients	385:392	arg1	IBD					399:401	IBD	399:401	IBD	399:401	The intestinal microbiota also plays a key role in the pathogenesis of IBD, and iron supplementation likely influences gut bacterial diversity in patients with IBD.
30308045	7	7	theme	Firmicutes	1537:1546	arg1	abundance					1524:1532	faecal abundance	1517:1532	faecal abundance of Firmicutes and Bacteroidetes	1517:1564	In the presence of colitis, dietary iron at 400 ppm resulted in a significant reduction in faecal abundance of Firmicutes and Bacteroidetes, and increase of Proteobacteria, changes which were not observed with lower dietary intake of iron at 100 ppm.
30308045	7	8	located	observed	1622:1629	arg2	changes					1599:1605	changes	1599:1605	changes which were not observed with lower dietary intake of iron at 100 ppm	1599:1674	In the presence of colitis, dietary iron at 400 ppm resulted in a significant reduction in faecal abundance of Firmicutes and Bacteroidetes, and increase of Proteobacteria, changes which were not observed with lower dietary intake of iron at 100 ppm.
30308045	7	8	located	observed	1622:1629	arg1	ppm					1672:1674	100 ppm	1668:1674	100 ppm	1668:1674	In the presence of colitis, dietary iron at 400 ppm resulted in a significant reduction in faecal abundance of Firmicutes and Bacteroidetes, and increase of Proteobacteria, changes which were not observed with lower dietary intake of iron at 100 ppm.
30308045	7	8	located	observed	1622:1629	arg2	Proteobacteria					1583:1596	Proteobacteria	1583:1596	Proteobacteria	1583:1596	In the presence of colitis, dietary iron at 400 ppm resulted in a significant reduction in faecal abundance of Firmicutes and Bacteroidetes, and increase of Proteobacteria, changes which were not observed with lower dietary intake of iron at 100 ppm.
30308045	8	9	dep	bacteria	1822:1829	arg1	diversity					1831:1839	diversity	1831:1839	diversity	1831:1839	Overall, altering dietary iron intake exacerbated DSS-induced colitis; increasing the iron content of the diet also led to changes in intestinal bacteria diversity and composition after colitis was induced with DSS.
30308045	8	9	dep	bacteria	1822:1829	arg1	bacteria					1822:1829	intestinal bacteria diversity and composition	1811:1855	intestinal bacteria diversity and composition	1811:1855	Overall, altering dietary iron intake exacerbated DSS-induced colitis; increasing the iron content of the diet also led to changes in intestinal bacteria diversity and composition after colitis was induced with DSS.
30308045	8	9	dep	bacteria	1822:1829	arg1	composition					1845:1855	composition	1845:1855	composition	1845:1855	Overall, altering dietary iron intake exacerbated DSS-induced colitis; increasing the iron content of the diet also led to changes in intestinal bacteria diversity and composition after colitis was induced with DSS.
30308045	3	10	theme	clinical	639:646	arg1	severity					665:672	(i) clinical and histological severity	635:672	(i) clinical and histological severity of acute DSS-induced colitis	635:701	Here, we assessed the impact of dietary iron, using chow diets containing either 100, 200 or 400 ppm, fed ad libitum to adult female C57BL/6 mice in the presence or absence of colitis induced using dextran sulfate sodium (DSS), on (i) clinical and histological severity of acute DSS-induced colitis, and (ii) faecal microbial diversity, as assessed by sequencing the V4 region of 16S rRNA.
30308045	3	11	theme	ad	510:511	arg1	libitum					513:519	fed ad libitum	506:519	fed ad libitum to adult female C57BL/6 mice in the presence or absence of colitis induced using dextran sulfate sodium (DSS), on (i) clinical and histological severity of acute DSS-induced colitis	506:701	Here, we assessed the impact of dietary iron, using chow diets containing either 100, 200 or 400 ppm, fed ad libitum to adult female C57BL/6 mice in the presence or absence of colitis induced using dextran sulfate sodium (DSS), on (i) clinical and histological severity of acute DSS-induced colitis, and (ii) faecal microbial diversity, as assessed by sequencing the V4 region of 16S rRNA.
30308045	3	11	theme	ad	510:511	arg1	ppm					501:503	100, 200 or 400 ppm	485:503	100, 200 or 400 ppm	485:503	Here, we assessed the impact of dietary iron, using chow diets containing either 100, 200 or 400 ppm, fed ad libitum to adult female C57BL/6 mice in the presence or absence of colitis induced using dextran sulfate sodium (DSS), on (i) clinical and histological severity of acute DSS-induced colitis, and (ii) faecal microbial diversity, as assessed by sequencing the V4 region of 16S rRNA.
30308045	6	12	theme	Faecal	1161:1166	arg1	levels					1181:1186	Faecal calprotectin levels	1161:1186	Faecal calprotectin levels	1161:1186	Faecal calprotectin levels were significantly increased in the presence of colitis in those consuming 100 ppm iron at day 8 (5.94-fold) versus day-10 group (4.14-fold) (p<0.05), and for the 400 ppm day-8 group (8.17-fold) versus day-10 group (4.44-fold) (p<0.001).
30308045	3	13	theme	female	530:535	arg1	mice					545:548	adult female C57BL/6 mice	524:548	adult female C57BL/6 mice	524:548	Here, we assessed the impact of dietary iron, using chow diets containing either 100, 200 or 400 ppm, fed ad libitum to adult female C57BL/6 mice in the presence or absence of colitis induced using dextran sulfate sodium (DSS), on (i) clinical and histological severity of acute DSS-induced colitis, and (ii) faecal microbial diversity, as assessed by sequencing the V4 region of 16S rRNA.
30308045	3	14	theme	histological	652:663	arg1	severity					665:672	(i) clinical and histological severity	635:672	(i) clinical and histological severity of acute DSS-induced colitis	635:701	Here, we assessed the impact of dietary iron, using chow diets containing either 100, 200 or 400 ppm, fed ad libitum to adult female C57BL/6 mice in the presence or absence of colitis induced using dextran sulfate sodium (DSS), on (i) clinical and histological severity of acute DSS-induced colitis, and (ii) faecal microbial diversity, as assessed by sequencing the V4 region of 16S rRNA.
30308045	7	15	theme	Bacteroidetes	1552:1564	arg1	abundance					1524:1532	faecal abundance	1517:1532	faecal abundance of Firmicutes and Bacteroidetes	1517:1564	In the presence of colitis, dietary iron at 400 ppm resulted in a significant reduction in faecal abundance of Firmicutes and Bacteroidetes, and increase of Proteobacteria, changes which were not observed with lower dietary intake of iron at 100 ppm.
30308045	2	16	theme	intestinal	243:252	arg1	microbiota					254:263	The intestinal microbiota	239:263	The intestinal microbiota	239:263	The intestinal microbiota also plays a key role in the pathogenesis of IBD, and iron supplementation likely influences gut bacterial diversity in patients with IBD.
30308045	3	17	theme	microbial	720:728	arg1	diversity					730:738	(ii) faecal microbial diversity	708:738	(ii) faecal microbial diversity	708:738	Here, we assessed the impact of dietary iron, using chow diets containing either 100, 200 or 400 ppm, fed ad libitum to adult female C57BL/6 mice in the presence or absence of colitis induced using dextran sulfate sodium (DSS), on (i) clinical and histological severity of acute DSS-induced colitis, and (ii) faecal microbial diversity, as assessed by sequencing the V4 region of 16S rRNA.
30308045	3	18	theme	DSS-induced	683:693	arg1	colitis					695:701	acute DSS-induced colitis	677:701	acute DSS-induced colitis	677:701	Here, we assessed the impact of dietary iron, using chow diets containing either 100, 200 or 400 ppm, fed ad libitum to adult female C57BL/6 mice in the presence or absence of colitis induced using dextran sulfate sodium (DSS), on (i) clinical and histological severity of acute DSS-induced colitis, and (ii) faecal microbial diversity, as assessed by sequencing the V4 region of 16S rRNA.
30308045	6	19	theme	colitis	1236:1242	arg1	presence					1224:1231	the presence	1220:1231	the presence of colitis	1220:1242	Faecal calprotectin levels were significantly increased in the presence of colitis in those consuming 100 ppm iron at day 8 (5.94-fold) versus day-10 group (4.14-fold) (p<0.05), and for the 400 ppm day-8 group (8.17-fold) versus day-10 group (4.44-fold) (p<0.001).
30308045	5	20	theme	iron	1007:1010	arg1	libitum					1015:1021	iron ad libitum	1007:1021	iron ad libitum	1007:1021	DSS-treated mice provided only half the standard levels of iron ad libitum (i.e. chow containing 100 ppm iron) lost more body weight than those receiving double the amount of standard iron (i.e. 400 ppm); p<0.01.
30308045	6	21	theme	ppm	1355:1357	arg1	8.17-fold					1372:1380	8.17-fold	1372:1380	8.17-fold	1372:1380	Faecal calprotectin levels were significantly increased in the presence of colitis in those consuming 100 ppm iron at day 8 (5.94-fold) versus day-10 group (4.14-fold) (p<0.05), and for the 400 ppm day-8 group (8.17-fold) versus day-10 group (4.44-fold) (p<0.001).
30308045	6	21	theme	ppm	1355:1357	arg1	group					1365:1369	the 400 ppm day-8 group	1347:1369	the 400 ppm day-8 group (8.17-fold)	1347:1381	Faecal calprotectin levels were significantly increased in the presence of colitis in those consuming 100 ppm iron at day 8 (5.94-fold) versus day-10 group (4.14-fold) (p<0.05), and for the 400 ppm day-8 group (8.17-fold) versus day-10 group (4.44-fold) (p<0.001).
30308045	1	22	theme	oral	136:139	arg1	replacement					146:156	oral iron replacement	136:156	oral iron replacement	136:156	Inflammatory bowel disease (IBD) is associated with anaemia and oral iron replacement to correct this can be problematic, intensifying inflammation and tissue damage.
30308045	5	23	theme	libitum	1015:1021	arg1	levels					997:1002	the standard levels	984:1002	the standard levels of iron ad libitum (i.e. chow containing 100 ppm iron)	984:1057	DSS-treated mice provided only half the standard levels of iron ad libitum (i.e. chow containing 100 ppm iron) lost more body weight than those receiving double the amount of standard iron (i.e. 400 ppm); p<0.01.
30308045	4	24	theme	dietary	819:825	arg1	concentration					832:844	dietary iron concentration	819:844	dietary iron concentration from the standard 200 ppm exacerbated both clinical	819:896	Increasing or decreasing dietary iron concentration from the standard 200 ppm exacerbated both clinical and histological severity of DSS-induced colitis.
30308045	5	25	theme	iron	1132:1135	arg1	amount					1113:1118	double the amount	1102:1118	double the amount of standard iron (i.e. 400 ppm)	1102:1150	DSS-treated mice provided only half the standard levels of iron ad libitum (i.e. chow containing 100 ppm iron) lost more body weight than those receiving double the amount of standard iron (i.e. 400 ppm); p<0.01.
30308045	5	25	theme	iron	1132:1135	arg1	iron					1132:1135	standard iron	1123:1135	standard iron (i.e. 400 ppm)	1123:1150	DSS-treated mice provided only half the standard levels of iron ad libitum (i.e. chow containing 100 ppm iron) lost more body weight than those receiving double the amount of standard iron (i.e. 400 ppm); p<0.01.
30308045	5	25	theme	iron	1132:1135	arg1	ppm					1147:1149	400 ppm	1143:1149	400 ppm	1143:1149	DSS-treated mice provided only half the standard levels of iron ad libitum (i.e. chow containing 100 ppm iron) lost more body weight than those receiving double the amount of standard iron (i.e. 400 ppm); p<0.01.
30308045	8	26	theme	DSS-induced	1727:1737	arg1	colitis					1739:1745	DSS-induced colitis	1727:1745	DSS-induced colitis	1727:1745	Overall, altering dietary iron intake exacerbated DSS-induced colitis; increasing the iron content of the diet also led to changes in intestinal bacteria diversity and composition after colitis was induced with DSS.
30308045	8	27	theme	diet	1783:1786	arg1	content					1768:1774	the iron content	1759:1774	the iron content of the diet	1759:1786	Overall, altering dietary iron intake exacerbated DSS-induced colitis; increasing the iron content of the diet also led to changes in intestinal bacteria diversity and composition after colitis was induced with DSS.
30308045	1	28	theme	bowel	85:89	arg1	IBD					100:102	IBD	100:102	IBD	100:102	Inflammatory bowel disease (IBD) is associated with anaemia and oral iron replacement to correct this can be problematic, intensifying inflammation and tissue damage.
30308045	1	28	theme	bowel	85:89	arg1	disease					91:97	Inflammatory bowel disease	72:97	Inflammatory bowel disease (IBD)	72:103	Inflammatory bowel disease (IBD) is associated with anaemia and oral iron replacement to correct this can be problematic, intensifying inflammation and tissue damage.
30308045	3	29	theme	V4	771:772	arg1	region					774:779	the V4 region	767:779	sequencing the V4 region of 16S rRNA	756:791	Here, we assessed the impact of dietary iron, using chow diets containing either 100, 200 or 400 ppm, fed ad libitum to adult female C57BL/6 mice in the presence or absence of colitis induced using dextran sulfate sodium (DSS), on (i) clinical and histological severity of acute DSS-induced colitis, and (ii) faecal microbial diversity, as assessed by sequencing the V4 region of 16S rRNA.
30308045	4	30	theme	histological	902:913	arg1	severity					915:922	histological severity	902:922	histological severity of DSS-induced colitis	902:945	Increasing or decreasing dietary iron concentration from the standard 200 ppm exacerbated both clinical and histological severity of DSS-induced colitis.
30308045	5	31	dep	chow	1029:1032	arg1	i.e.					1024:1027	i.e.	1024:1027	i.e.	1024:1027	DSS-treated mice provided only half the standard levels of iron ad libitum (i.e. chow containing 100 ppm iron) lost more body weight than those receiving double the amount of standard iron (i.e. 400 ppm); p<0.01.
30308045	2	32	theme	bacterial	362:370	arg1	diversity					372:380	gut bacterial diversity	358:380	gut bacterial diversity in patients with IBD	358:401	The intestinal microbiota also plays a key role in the pathogenesis of IBD, and iron supplementation likely influences gut bacterial diversity in patients with IBD.
30308045	0	33	theme	Oral	0:3	arg1	iron					5:8	Oral iron	0:8	Oral iron	0:8	Oral iron exacerbates colitis and influences the intestinal microbiome.
30308045	5	34	theme	ppm	1049:1051	arg1	iron					1053:1056	100 ppm iron	1045:1056	100 ppm iron	1045:1056	DSS-treated mice provided only half the standard levels of iron ad libitum (i.e. chow containing 100 ppm iron) lost more body weight than those receiving double the amount of standard iron (i.e. 400 ppm); p<0.01.
30308045	3	35	dep	sequencing	756:765	arg1	region					774:779	the V4 region	767:779	sequencing the V4 region of 16S rRNA	756:791	Here, we assessed the impact of dietary iron, using chow diets containing either 100, 200 or 400 ppm, fed ad libitum to adult female C57BL/6 mice in the presence or absence of colitis induced using dextran sulfate sodium (DSS), on (i) clinical and histological severity of acute DSS-induced colitis, and (ii) faecal microbial diversity, as assessed by sequencing the V4 region of 16S rRNA.
30308045	3	36	theme	rRNA	788:791	arg1	region					774:779	the V4 region	767:779	sequencing the V4 region of 16S rRNA	756:791	Here, we assessed the impact of dietary iron, using chow diets containing either 100, 200 or 400 ppm, fed ad libitum to adult female C57BL/6 mice in the presence or absence of colitis induced using dextran sulfate sodium (DSS), on (i) clinical and histological severity of acute DSS-induced colitis, and (ii) faecal microbial diversity, as assessed by sequencing the V4 region of 16S rRNA.
30308045	4	37	theme	exacerbated	872:882	arg1	ppm					868:870	the standard 200 ppm	851:870	the standard 200 ppm exacerbated both clinical	851:896	Increasing or decreasing dietary iron concentration from the standard 200 ppm exacerbated both clinical and histological severity of DSS-induced colitis.
30308045	6	38	theme	100	1263:1265	arg1	ppm					1267:1269	ppm	1267:1269	ppm	1267:1269	Faecal calprotectin levels were significantly increased in the presence of colitis in those consuming 100 ppm iron at day 8 (5.94-fold) versus day-10 group (4.14-fold) (p<0.05), and for the 400 ppm day-8 group (8.17-fold) versus day-10 group (4.44-fold) (p<0.001).
30308045	7	39	theme	dietary	1642:1648	arg1	intake					1650:1655	lower dietary intake	1636:1655	lower dietary intake of iron	1636:1663	In the presence of colitis, dietary iron at 400 ppm resulted in a significant reduction in faecal abundance of Firmicutes and Bacteroidetes, and increase of Proteobacteria, changes which were not observed with lower dietary intake of iron at 100 ppm.
30308045	8	40	theme	dietary	1695:1701	arg1	intake					1708:1713	altering dietary iron intake	1686:1713	altering dietary iron intake	1686:1713	Overall, altering dietary iron intake exacerbated DSS-induced colitis; increasing the iron content of the diet also led to changes in intestinal bacteria diversity and composition after colitis was induced with DSS.
30308045	7	41	theme	Proteobacteria	1583:1596	arg1	increase					1571:1578	increase	1571:1578	increase of Proteobacteria, changes which were not observed with lower dietary intake of iron at 100 ppm	1571:1674	In the presence of colitis, dietary iron at 400 ppm resulted in a significant reduction in faecal abundance of Firmicutes and Bacteroidetes, and increase of Proteobacteria, changes which were not observed with lower dietary intake of iron at 100 ppm.
30308045	7	41	theme	Proteobacteria	1583:1596	arg1	reduction					1504:1512	a significant reduction	1490:1512	a significant reduction in faecal abundance of Firmicutes and Bacteroidetes	1490:1564	In the presence of colitis, dietary iron at 400 ppm resulted in a significant reduction in faecal abundance of Firmicutes and Bacteroidetes, and increase of Proteobacteria, changes which were not observed with lower dietary intake of iron at 100 ppm.
30308045	3	42	theme	dextran	602:608	arg1	DSS					626:628	DSS	626:628	DSS	626:628	Here, we assessed the impact of dietary iron, using chow diets containing either 100, 200 or 400 ppm, fed ad libitum to adult female C57BL/6 mice in the presence or absence of colitis induced using dextran sulfate sodium (DSS), on (i) clinical and histological severity of acute DSS-induced colitis, and (ii) faecal microbial diversity, as assessed by sequencing the V4 region of 16S rRNA.
30308045	3	42	theme	dextran	602:608	arg1	sodium					618:623	dextran sulfate sodium	602:623	dextran sulfate sodium (DSS)	602:629	Here, we assessed the impact of dietary iron, using chow diets containing either 100, 200 or 400 ppm, fed ad libitum to adult female C57BL/6 mice in the presence or absence of colitis induced using dextran sulfate sodium (DSS), on (i) clinical and histological severity of acute DSS-induced colitis, and (ii) faecal microbial diversity, as assessed by sequencing the V4 region of 16S rRNA.
30308045	2	43	theme	IBD	310:312	arg1	pathogenesis					294:305	the pathogenesis	290:305	the pathogenesis of IBD	290:312	The intestinal microbiota also plays a key role in the pathogenesis of IBD, and iron supplementation likely influences gut bacterial diversity in patients with IBD.
30308045	5	44	dep	half	979:982	arg1	lost					1059:1062	lost	1059:1062	lost more body weight than those receiving double the amount of standard iron (i.e. 400 ppm); p<0.01	1059:1158	DSS-treated mice provided only half the standard levels of iron ad libitum (i.e. chow containing 100 ppm iron) lost more body weight than those receiving double the amount of standard iron (i.e. 400 ppm); p<0.01.
30308045	5	45	theme	double	1102:1107	arg1	amount					1113:1118	double the amount	1102:1118	double the amount of standard iron (i.e. 400 ppm)	1102:1150	DSS-treated mice provided only half the standard levels of iron ad libitum (i.e. chow containing 100 ppm iron) lost more body weight than those receiving double the amount of standard iron (i.e. 400 ppm); p<0.01.
30308045	5	45	theme	double	1102:1107	arg1	iron					1132:1135	standard iron	1123:1135	standard iron (i.e. 400 ppm)	1123:1150	DSS-treated mice provided only half the standard levels of iron ad libitum (i.e. chow containing 100 ppm iron) lost more body weight than those receiving double the amount of standard iron (i.e. 400 ppm); p<0.01.
30308045	5	45	theme	double	1102:1107	arg1	ppm					1147:1149	400 ppm	1143:1149	400 ppm	1143:1149	DSS-treated mice provided only half the standard levels of iron ad libitum (i.e. chow containing 100 ppm iron) lost more body weight than those receiving double the amount of standard iron (i.e. 400 ppm); p<0.01.
30308045	4	46	theme	colitis	939:945	arg1	concentration					832:844	dietary iron concentration	819:844	dietary iron concentration from the standard 200 ppm exacerbated both clinical	819:896	Increasing or decreasing dietary iron concentration from the standard 200 ppm exacerbated both clinical and histological severity of DSS-induced colitis.
30308045	4	46	theme	colitis	939:945	arg1	severity					915:922	histological severity	902:922	histological severity of DSS-induced colitis	902:945	Increasing or decreasing dietary iron concentration from the standard 200 ppm exacerbated both clinical and histological severity of DSS-induced colitis.
30308045	5	47	theme	more	1064:1067	arg1	weight					1074:1079	more body weight	1064:1079	more body weight than those receiving double the amount of standard iron (i.e. 400 ppm); p<0.01	1064:1158	DSS-treated mice provided only half the standard levels of iron ad libitum (i.e. chow containing 100 ppm iron) lost more body weight than those receiving double the amount of standard iron (i.e. 400 ppm); p<0.01.
30308045	4	48	dep	exacerbated	872:882	arg1	both					884:887	both	884:887	both	884:887	Increasing or decreasing dietary iron concentration from the standard 200 ppm exacerbated both clinical and histological severity of DSS-induced colitis.
30308045	2	49	theme	key	278:280	arg1	role					282:285	a key role	276:285	a key role	276:285	The intestinal microbiota also plays a key role in the pathogenesis of IBD, and iron supplementation likely influences gut bacterial diversity in patients with IBD.
30308045	7	50	theme	faecal	1517:1522	arg1	abundance					1524:1532	faecal abundance	1517:1532	faecal abundance of Firmicutes and Bacteroidetes	1517:1564	In the presence of colitis, dietary iron at 400 ppm resulted in a significant reduction in faecal abundance of Firmicutes and Bacteroidetes, and increase of Proteobacteria, changes which were not observed with lower dietary intake of iron at 100 ppm.
30308045	7	51	from	ppm	1474:1476	arg1	iron					1462:1465	dietary iron	1454:1465	dietary iron at 400 ppm	1454:1476	In the presence of colitis, dietary iron at 400 ppm resulted in a significant reduction in faecal abundance of Firmicutes and Bacteroidetes, and increase of Proteobacteria, changes which were not observed with lower dietary intake of iron at 100 ppm.
30308045	3	52	theme	fed	506:508	arg1	libitum					513:519	fed ad libitum	506:519	fed ad libitum to adult female C57BL/6 mice in the presence or absence of colitis induced using dextran sulfate sodium (DSS), on (i) clinical and histological severity of acute DSS-induced colitis	506:701	Here, we assessed the impact of dietary iron, using chow diets containing either 100, 200 or 400 ppm, fed ad libitum to adult female C57BL/6 mice in the presence or absence of colitis induced using dextran sulfate sodium (DSS), on (i) clinical and histological severity of acute DSS-induced colitis, and (ii) faecal microbial diversity, as assessed by sequencing the V4 region of 16S rRNA.
30308045	3	52	theme	fed	506:508	arg1	ppm					501:503	100, 200 or 400 ppm	485:503	100, 200 or 400 ppm	485:503	Here, we assessed the impact of dietary iron, using chow diets containing either 100, 200 or 400 ppm, fed ad libitum to adult female C57BL/6 mice in the presence or absence of colitis induced using dextran sulfate sodium (DSS), on (i) clinical and histological severity of acute DSS-induced colitis, and (ii) faecal microbial diversity, as assessed by sequencing the V4 region of 16S rRNA.
30308045	8	53	from	changes	1800:1806	arg1	diversity					1831:1839	diversity	1831:1839	diversity	1831:1839	Overall, altering dietary iron intake exacerbated DSS-induced colitis; increasing the iron content of the diet also led to changes in intestinal bacteria diversity and composition after colitis was induced with DSS.
30308045	8	53	from	changes	1800:1806	arg1	bacteria					1822:1829	intestinal bacteria diversity and composition	1811:1855	intestinal bacteria diversity and composition	1811:1855	Overall, altering dietary iron intake exacerbated DSS-induced colitis; increasing the iron content of the diet also led to changes in intestinal bacteria diversity and composition after colitis was induced with DSS.
30308045	8	53	from	changes	1800:1806	arg1	composition					1845:1855	composition	1845:1855	composition	1845:1855	Overall, altering dietary iron intake exacerbated DSS-induced colitis; increasing the iron content of the diet also led to changes in intestinal bacteria diversity and composition after colitis was induced with DSS.
30308045	6	54	theme	calprotectin	1168:1179	arg1	levels					1181:1186	Faecal calprotectin levels	1161:1186	Faecal calprotectin levels	1161:1186	Faecal calprotectin levels were significantly increased in the presence of colitis in those consuming 100 ppm iron at day 8 (5.94-fold) versus day-10 group (4.14-fold) (p<0.05), and for the 400 ppm day-8 group (8.17-fold) versus day-10 group (4.44-fold) (p<0.001).
30308045	3	55	theme	adult	524:528	arg1	mice					545:548	adult female C57BL/6 mice	524:548	adult female C57BL/6 mice	524:548	Here, we assessed the impact of dietary iron, using chow diets containing either 100, 200 or 400 ppm, fed ad libitum to adult female C57BL/6 mice in the presence or absence of colitis induced using dextran sulfate sodium (DSS), on (i) clinical and histological severity of acute DSS-induced colitis, and (ii) faecal microbial diversity, as assessed by sequencing the V4 region of 16S rRNA.
30308045	4	56	from	ppm	868:870	arg1	concentration					832:844	dietary iron concentration	819:844	dietary iron concentration from the standard 200 ppm exacerbated both clinical	819:896	Increasing or decreasing dietary iron concentration from the standard 200 ppm exacerbated both clinical and histological severity of DSS-induced colitis.
30308045	4	56	from	ppm	868:870	arg1	severity					915:922	histological severity	902:922	histological severity of DSS-induced colitis	902:945	Increasing or decreasing dietary iron concentration from the standard 200 ppm exacerbated both clinical and histological severity of DSS-induced colitis.
30308045	3	57	theme	acute	677:681	arg1	colitis					695:701	acute DSS-induced colitis	677:701	acute DSS-induced colitis	677:701	Here, we assessed the impact of dietary iron, using chow diets containing either 100, 200 or 400 ppm, fed ad libitum to adult female C57BL/6 mice in the presence or absence of colitis induced using dextran sulfate sodium (DSS), on (i) clinical and histological severity of acute DSS-induced colitis, and (ii) faecal microbial diversity, as assessed by sequencing the V4 region of 16S rRNA.
30308045	6	58	from	day	1279:1281	arg1	iron					1271:1274	100 ppm iron	1263:1274	100 ppm iron at day 8 (5.94-fold) versus day-10 group (4.14-fold) (p<0.05), and for the 400 ppm day-8 group (8.17-fold) versus day-10 group (4.44-fold) (p<0.001)	1263:1423	Faecal calprotectin levels were significantly increased in the presence of colitis in those consuming 100 ppm iron at day 8 (5.94-fold) versus day-10 group (4.14-fold) (p<0.05), and for the 400 ppm day-8 group (8.17-fold) versus day-10 group (4.44-fold) (p<0.001).
30308045	5	59	contain	containing	1034:1043	arg2	iron					1053:1056	100 ppm iron	1045:1056	100 ppm iron	1045:1056	DSS-treated mice provided only half the standard levels of iron ad libitum (i.e. chow containing 100 ppm iron) lost more body weight than those receiving double the amount of standard iron (i.e. 400 ppm); p<0.01.
30308045	5	59	contain	containing	1034:1043	arg1	chow					1029:1032	chow	1029:1032	chow containing 100 ppm iron	1029:1056	DSS-treated mice provided only half the standard levels of iron ad libitum (i.e. chow containing 100 ppm iron) lost more body weight than those receiving double the amount of standard iron (i.e. 400 ppm); p<0.01.
30308045	5	60	dep	weight	1074:1079	arg1	p<0.01					1153:1158	p<0.01	1153:1158	more body weight than those receiving double the amount of standard iron (i.e. 400 ppm); p<0.01	1064:1158	DSS-treated mice provided only half the standard levels of iron ad libitum (i.e. chow containing 100 ppm iron) lost more body weight than those receiving double the amount of standard iron (i.e. 400 ppm); p<0.01.
30308045	8	61	theme	altering	1686:1693	arg1	intake					1708:1713	altering dietary iron intake	1686:1713	altering dietary iron intake	1686:1713	Overall, altering dietary iron intake exacerbated DSS-induced colitis; increasing the iron content of the diet also led to changes in intestinal bacteria diversity and composition after colitis was induced with DSS.
30308045	7	62	theme	significant	1492:1502	arg1	reduction					1504:1512	a significant reduction	1490:1512	a significant reduction in faecal abundance of Firmicutes and Bacteroidetes	1490:1564	In the presence of colitis, dietary iron at 400 ppm resulted in a significant reduction in faecal abundance of Firmicutes and Bacteroidetes, and increase of Proteobacteria, changes which were not observed with lower dietary intake of iron at 100 ppm.
30308045	3	63	theme	iron	444:447	arg1	impact					426:431	the impact	422:431	the impact of dietary iron	422:447	Here, we assessed the impact of dietary iron, using chow diets containing either 100, 200 or 400 ppm, fed ad libitum to adult female C57BL/6 mice in the presence or absence of colitis induced using dextran sulfate sodium (DSS), on (i) clinical and histological severity of acute DSS-induced colitis, and (ii) faecal microbial diversity, as assessed by sequencing the V4 region of 16S rRNA.
30308045	8	64	theme	iron	1703:1706	arg1	intake					1708:1713	altering dietary iron intake	1686:1713	altering dietary iron intake	1686:1713	Overall, altering dietary iron intake exacerbated DSS-induced colitis; increasing the iron content of the diet also led to changes in intestinal bacteria diversity and composition after colitis was induced with DSS.
30308045	5	65	theme	DSS-treated	948:958	arg1	mice					960:963	DSS-treated mice	948:963	DSS-treated mice	948:963	DSS-treated mice provided only half the standard levels of iron ad libitum (i.e. chow containing 100 ppm iron) lost more body weight than those receiving double the amount of standard iron (i.e. 400 ppm); p<0.01.
30308045	3	66	theme	C57BL/6	537:543	arg1	mice					545:548	adult female C57BL/6 mice	524:548	adult female C57BL/6 mice	524:548	Here, we assessed the impact of dietary iron, using chow diets containing either 100, 200 or 400 ppm, fed ad libitum to adult female C57BL/6 mice in the presence or absence of colitis induced using dextran sulfate sodium (DSS), on (i) clinical and histological severity of acute DSS-induced colitis, and (ii) faecal microbial diversity, as assessed by sequencing the V4 region of 16S rRNA.
30308045	6	67	theme	day-8	1359:1363	arg1	8.17-fold					1372:1380	8.17-fold	1372:1380	8.17-fold	1372:1380	Faecal calprotectin levels were significantly increased in the presence of colitis in those consuming 100 ppm iron at day 8 (5.94-fold) versus day-10 group (4.14-fold) (p<0.05), and for the 400 ppm day-8 group (8.17-fold) versus day-10 group (4.44-fold) (p<0.001).
30308045	6	67	theme	day-8	1359:1363	arg1	group					1365:1369	the 400 ppm day-8 group	1347:1369	the 400 ppm day-8 group (8.17-fold)	1347:1381	Faecal calprotectin levels were significantly increased in the presence of colitis in those consuming 100 ppm iron at day 8 (5.94-fold) versus day-10 group (4.14-fold) (p<0.05), and for the 400 ppm day-8 group (8.17-fold) versus day-10 group (4.44-fold) (p<0.001).
30308045	3	68	contain	containing	467:476	arg2	libitum					513:519	fed ad libitum	506:519	fed ad libitum to adult female C57BL/6 mice in the presence or absence of colitis induced using dextran sulfate sodium (DSS), on (i) clinical and histological severity of acute DSS-induced colitis	506:701	Here, we assessed the impact of dietary iron, using chow diets containing either 100, 200 or 400 ppm, fed ad libitum to adult female C57BL/6 mice in the presence or absence of colitis induced using dextran sulfate sodium (DSS), on (i) clinical and histological severity of acute DSS-induced colitis, and (ii) faecal microbial diversity, as assessed by sequencing the V4 region of 16S rRNA.
30308045	3	68	contain	containing	467:476	arg2	diversity					730:738	(ii) faecal microbial diversity	708:738	(ii) faecal microbial diversity	708:738	Here, we assessed the impact of dietary iron, using chow diets containing either 100, 200 or 400 ppm, fed ad libitum to adult female C57BL/6 mice in the presence or absence of colitis induced using dextran sulfate sodium (DSS), on (i) clinical and histological severity of acute DSS-induced colitis, and (ii) faecal microbial diversity, as assessed by sequencing the V4 region of 16S rRNA.
30308045	3	68	contain	containing	467:476	arg1	diets					461:465	chow diets	456:465	chow diets containing either 100, 200 or 400 ppm, fed ad libitum to adult female C57BL/6 mice in the presence or absence of colitis induced using dextran sulfate sodium (DSS), on (i) clinical and histological severity of acute DSS-induced colitis, and (ii) faecal microbial diversity	456:738	Here, we assessed the impact of dietary iron, using chow diets containing either 100, 200 or 400 ppm, fed ad libitum to adult female C57BL/6 mice in the presence or absence of colitis induced using dextran sulfate sodium (DSS), on (i) clinical and histological severity of acute DSS-induced colitis, and (ii) faecal microbial diversity, as assessed by sequencing the V4 region of 16S rRNA.
30308045	3	68	contain	containing	467:476	arg2	ppm					501:503	100, 200 or 400 ppm	485:503	100, 200 or 400 ppm	485:503	Here, we assessed the impact of dietary iron, using chow diets containing either 100, 200 or 400 ppm, fed ad libitum to adult female C57BL/6 mice in the presence or absence of colitis induced using dextran sulfate sodium (DSS), on (i) clinical and histological severity of acute DSS-induced colitis, and (ii) faecal microbial diversity, as assessed by sequencing the V4 region of 16S rRNA.
30308045	3	69	theme	colitis	695:701	arg1	severity					665:672	(i) clinical and histological severity	635:672	(i) clinical and histological severity of acute DSS-induced colitis	635:701	Here, we assessed the impact of dietary iron, using chow diets containing either 100, 200 or 400 ppm, fed ad libitum to adult female C57BL/6 mice in the presence or absence of colitis induced using dextran sulfate sodium (DSS), on (i) clinical and histological severity of acute DSS-induced colitis, and (ii) faecal microbial diversity, as assessed by sequencing the V4 region of 16S rRNA.
30308045	5	70	theme	ad	1012:1013	arg1	libitum					1015:1021	iron ad libitum	1007:1021	iron ad libitum	1007:1021	DSS-treated mice provided only half the standard levels of iron ad libitum (i.e. chow containing 100 ppm iron) lost more body weight than those receiving double the amount of standard iron (i.e. 400 ppm); p<0.01.
30308045	6	71	theme	400	1351:1353	arg1	ppm					1355:1357	ppm	1355:1357	ppm	1355:1357	Faecal calprotectin levels were significantly increased in the presence of colitis in those consuming 100 ppm iron at day 8 (5.94-fold) versus day-10 group (4.14-fold) (p<0.05), and for the 400 ppm day-8 group (8.17-fold) versus day-10 group (4.44-fold) (p<0.001).
30308045	1	72	theme	iron	141:144	arg1	replacement					146:156	oral iron replacement	136:156	oral iron replacement	136:156	Inflammatory bowel disease (IBD) is associated with anaemia and oral iron replacement to correct this can be problematic, intensifying inflammation and tissue damage.
30308045	4	73	theme	standard	855:862	arg1	ppm					868:870	the standard 200 ppm	851:870	the standard 200 ppm exacerbated both clinical	851:896	Increasing or decreasing dietary iron concentration from the standard 200 ppm exacerbated both clinical and histological severity of DSS-induced colitis.
30308045	5	74	theme	standard	1123:1130	arg1	iron					1132:1135	standard iron	1123:1135	standard iron (i.e. 400 ppm)	1123:1150	DSS-treated mice provided only half the standard levels of iron ad libitum (i.e. chow containing 100 ppm iron) lost more body weight than those receiving double the amount of standard iron (i.e. 400 ppm); p<0.01.
30308045	5	74	theme	standard	1123:1130	arg1	ppm					1147:1149	400 ppm	1143:1149	400 ppm	1143:1149	DSS-treated mice provided only half the standard levels of iron ad libitum (i.e. chow containing 100 ppm iron) lost more body weight than those receiving double the amount of standard iron (i.e. 400 ppm); p<0.01.
30308045	3	75	theme	faecal	713:718	arg1	diversity					730:738	(ii) faecal microbial diversity	708:738	(ii) faecal microbial diversity	708:738	Here, we assessed the impact of dietary iron, using chow diets containing either 100, 200 or 400 ppm, fed ad libitum to adult female C57BL/6 mice in the presence or absence of colitis induced using dextran sulfate sodium (DSS), on (i) clinical and histological severity of acute DSS-induced colitis, and (ii) faecal microbial diversity, as assessed by sequencing the V4 region of 16S rRNA.
30308045	5	76	theme	standard	988:995	arg1	levels					997:1002	the standard levels	984:1002	the standard levels of iron ad libitum (i.e. chow containing 100 ppm iron)	984:1057	DSS-treated mice provided only half the standard levels of iron ad libitum (i.e. chow containing 100 ppm iron) lost more body weight than those receiving double the amount of standard iron (i.e. 400 ppm); p<0.01.
30308045	1	77	theme	Inflammatory	72:83	arg1	IBD					100:102	IBD	100:102	IBD	100:102	Inflammatory bowel disease (IBD) is associated with anaemia and oral iron replacement to correct this can be problematic, intensifying inflammation and tissue damage.
30308045	1	77	theme	Inflammatory	72:83	arg1	disease					91:97	Inflammatory bowel disease	72:97	Inflammatory bowel disease (IBD)	72:103	Inflammatory bowel disease (IBD) is associated with anaemia and oral iron replacement to correct this can be problematic, intensifying inflammation and tissue damage.
30308045	3	78	theme	16S	784:786	arg1	rRNA					788:791	16S rRNA	784:791	16S rRNA	784:791	Here, we assessed the impact of dietary iron, using chow diets containing either 100, 200 or 400 ppm, fed ad libitum to adult female C57BL/6 mice in the presence or absence of colitis induced using dextran sulfate sodium (DSS), on (i) clinical and histological severity of acute DSS-induced colitis, and (ii) faecal microbial diversity, as assessed by sequencing the V4 region of 16S rRNA.
30308045	4	79	theme	iron	827:830	arg1	concentration					832:844	dietary iron concentration	819:844	dietary iron concentration from the standard 200 ppm exacerbated both clinical	819:896	Increasing or decreasing dietary iron concentration from the standard 200 ppm exacerbated both clinical and histological severity of DSS-induced colitis.
30308045	8	80	theme	iron	1763:1766	arg1	content					1768:1774	the iron content	1759:1774	the iron content of the diet	1759:1786	Overall, altering dietary iron intake exacerbated DSS-induced colitis; increasing the iron content of the diet also led to changes in intestinal bacteria diversity and composition after colitis was induced with DSS.
30308045	7	81	theme	iron	1660:1663	arg1	intake					1650:1655	lower dietary intake	1636:1655	lower dietary intake of iron	1636:1663	In the presence of colitis, dietary iron at 400 ppm resulted in a significant reduction in faecal abundance of Firmicutes and Bacteroidetes, and increase of Proteobacteria, changes which were not observed with lower dietary intake of iron at 100 ppm.
30308045	7	82	theme	colitis	1445:1451	arg1	presence					1433:1440	the presence	1429:1440	the presence of colitis	1429:1451	In the presence of colitis, dietary iron at 400 ppm resulted in a significant reduction in faecal abundance of Firmicutes and Bacteroidetes, and increase of Proteobacteria, changes which were not observed with lower dietary intake of iron at 100 ppm.
30308045	7	83	from	increase	1571:1578	arg1	abundance					1524:1532	faecal abundance	1517:1532	faecal abundance of Firmicutes and Bacteroidetes	1517:1564	In the presence of colitis, dietary iron at 400 ppm resulted in a significant reduction in faecal abundance of Firmicutes and Bacteroidetes, and increase of Proteobacteria, changes which were not observed with lower dietary intake of iron at 100 ppm.
30308045	3	84	from	libitum	513:519	arg1	absence					569:575	absence	569:575	absence	569:575	Here, we assessed the impact of dietary iron, using chow diets containing either 100, 200 or 400 ppm, fed ad libitum to adult female C57BL/6 mice in the presence or absence of colitis induced using dextran sulfate sodium (DSS), on (i) clinical and histological severity of acute DSS-induced colitis, and (ii) faecal microbial diversity, as assessed by sequencing the V4 region of 16S rRNA.
30308045	3	84	from	libitum	513:519	arg1	presence					557:564	the presence	553:564	the presence	553:564	Here, we assessed the impact of dietary iron, using chow diets containing either 100, 200 or 400 ppm, fed ad libitum to adult female C57BL/6 mice in the presence or absence of colitis induced using dextran sulfate sodium (DSS), on (i) clinical and histological severity of acute DSS-induced colitis, and (ii) faecal microbial diversity, as assessed by sequencing the V4 region of 16S rRNA.
30308045	2	85	theme	gut	358:360	arg1	diversity					372:380	gut bacterial diversity	358:380	gut bacterial diversity in patients with IBD	358:401	The intestinal microbiota also plays a key role in the pathogenesis of IBD, and iron supplementation likely influences gut bacterial diversity in patients with IBD.
30308045	3	86	theme	dietary	436:442	arg1	iron					444:447	dietary iron	436:447	dietary iron	436:447	Here, we assessed the impact of dietary iron, using chow diets containing either 100, 200 or 400 ppm, fed ad libitum to adult female C57BL/6 mice in the presence or absence of colitis induced using dextran sulfate sodium (DSS), on (i) clinical and histological severity of acute DSS-induced colitis, and (ii) faecal microbial diversity, as assessed by sequencing the V4 region of 16S rRNA.
30308045	6	87	theme	ppm	1267:1269	arg1	iron					1271:1274	100 ppm iron	1263:1274	100 ppm iron at day 8 (5.94-fold) versus day-10 group (4.14-fold) (p<0.05), and for the 400 ppm day-8 group (8.17-fold) versus day-10 group (4.44-fold) (p<0.001)	1263:1423	Faecal calprotectin levels were significantly increased in the presence of colitis in those consuming 100 ppm iron at day 8 (5.94-fold) versus day-10 group (4.14-fold) (p<0.05), and for the 400 ppm day-8 group (8.17-fold) versus day-10 group (4.44-fold) (p<0.001).
30308045	7	88	theme	dietary	1454:1460	arg1	iron					1462:1465	dietary iron	1454:1465	dietary iron at 400 ppm	1454:1476	In the presence of colitis, dietary iron at 400 ppm resulted in a significant reduction in faecal abundance of Firmicutes and Bacteroidetes, and increase of Proteobacteria, changes which were not observed with lower dietary intake of iron at 100 ppm.
30308045	7	89	theme	lower	1636:1640	arg1	intake					1650:1655	lower dietary intake	1636:1655	lower dietary intake of iron	1636:1663	In the presence of colitis, dietary iron at 400 ppm resulted in a significant reduction in faecal abundance of Firmicutes and Bacteroidetes, and increase of Proteobacteria, changes which were not observed with lower dietary intake of iron at 100 ppm.
30308045	1	90	theme	tissue	224:229	arg1	damage					231:236	tissue damage	224:236	tissue damage	224:236	Inflammatory bowel disease (IBD) is associated with anaemia and oral iron replacement to correct this can be problematic, intensifying inflammation and tissue damage.
30308045	0	91	theme	intestinal	49:58	arg1	microbiome					60:69	the intestinal microbiome	45:69	the intestinal microbiome	45:69	Oral iron exacerbates colitis and influences the intestinal microbiome.
30308045	7	92	from	reduction	1504:1512	arg1	abundance					1524:1532	faecal abundance	1517:1532	faecal abundance of Firmicutes and Bacteroidetes	1517:1564	In the presence of colitis, dietary iron at 400 ppm resulted in a significant reduction in faecal abundance of Firmicutes and Bacteroidetes, and increase of Proteobacteria, changes which were not observed with lower dietary intake of iron at 100 ppm.
30308045	3	93	theme	chow	456:459	arg1	diets					461:465	chow diets	456:465	chow diets containing either 100, 200 or 400 ppm, fed ad libitum to adult female C57BL/6 mice in the presence or absence of colitis induced using dextran sulfate sodium (DSS), on (i) clinical and histological severity of acute DSS-induced colitis, and (ii) faecal microbial diversity	456:738	Here, we assessed the impact of dietary iron, using chow diets containing either 100, 200 or 400 ppm, fed ad libitum to adult female C57BL/6 mice in the presence or absence of colitis induced using dextran sulfate sodium (DSS), on (i) clinical and histological severity of acute DSS-induced colitis, and (ii) faecal microbial diversity, as assessed by sequencing the V4 region of 16S rRNA.
30308045	5	94	theme	100	1045:1047	arg1	ppm					1049:1051	ppm	1049:1051	ppm	1049:1051	DSS-treated mice provided only half the standard levels of iron ad libitum (i.e. chow containing 100 ppm iron) lost more body weight than those receiving double the amount of standard iron (i.e. 400 ppm); p<0.01.
30308045	2	95	from	diversity	372:380	arg1	patients					385:392	patients	385:392	patients with IBD	385:401	The intestinal microbiota also plays a key role in the pathogenesis of IBD, and iron supplementation likely influences gut bacterial diversity in patients with IBD.
30308045	3	96	theme	colitis	580:586	arg1	absence					569:575	absence	569:575	absence	569:575	Here, we assessed the impact of dietary iron, using chow diets containing either 100, 200 or 400 ppm, fed ad libitum to adult female C57BL/6 mice in the presence or absence of colitis induced using dextran sulfate sodium (DSS), on (i) clinical and histological severity of acute DSS-induced colitis, and (ii) faecal microbial diversity, as assessed by sequencing the V4 region of 16S rRNA.
30308045	3	96	theme	colitis	580:586	arg1	presence					557:564	the presence	553:564	the presence	553:564	Here, we assessed the impact of dietary iron, using chow diets containing either 100, 200 or 400 ppm, fed ad libitum to adult female C57BL/6 mice in the presence or absence of colitis induced using dextran sulfate sodium (DSS), on (i) clinical and histological severity of acute DSS-induced colitis, and (ii) faecal microbial diversity, as assessed by sequencing the V4 region of 16S rRNA.
31478650	6	0	from	synergy	1550:1556	arg1	associations					1625:1636	mineral-organic associations	1609:1636	mineral-organic associations	1609:1636	These molecular-scale determinations and nanoscale observations should substantially improve our understanding of how environmental factors influence the organic-mineral interfacial interactions through the synergy of collective noncovalent and/or covalent bonds in mineral-organic associations.
31478650	4	1	theme	noncovalent	917:927	arg1	bonds					929:933	the noncovalent bonds	913:933	the noncovalent bonds and that systematically changing ion compositions, ionic strength, and pH result in significant differences in organic-organic and organic-mineral binding energies	913:1097	Single-molecule binding and adhesion force of eDNA to mineral and to mineral adsorbed by protein/polysaccharide reveal the noncovalent bonds and that systematically changing ion compositions, ionic strength, and pH result in significant differences in organic-organic and organic-mineral binding energies.
31478650	5	2	theme	mineral-bound	1194:1206	arg1	molecules					1212:1220	mineral-bound DNA molecules	1194:1220	mineral-bound DNA molecules	1194:1220	Consistent with the bond-strength measurements, protein, rather than polysaccharide, promotes mineral-bound DNA molecules by ex situ AFM deposition observations in relatively high concentrations of divalent cation-containing acidic solutions.
31478650	2	3	theme	molecular	400:408	arg1	scales					410:415	nanometer and molecular scales	386:415	nanometer and molecular scales in environmentally relevant solutions	386:453	The mechanisms underlying DNA-mineral and DNA-protein/polysaccharide interactions at nanometer and molecular scales in environmentally relevant solutions remain uncertain.
31478650	3	4	theme	nanomechanical	745:758	arg1	mapping					760:766	PeakForce quantitative nanomechanical mapping	722:766	PeakForce quantitative nanomechanical mapping using DNA-decorated tips	722:791	Here, we present a model mineral-SOM system consisting of mineral (mica)-nucleic acid (environmental DNA, eDNA)/protein (bovine serum albumin)/polysaccharide (alginate), and combine atomic force microscopy (AFM)-based dynamic force spectroscopy and PeakForce quantitative nanomechanical mapping using DNA-decorated tips.
31478650	0	5	theme	DNA	101:103	arg1	Adsorption					73:82	the Adsorption	69:82	the Adsorption of Environmental DNA on Mica	69:111	Molecular-Scale Investigations Reveal Noncovalent Bonding Underlying the Adsorption of Environmental DNA on Mica.
31478650	3	6	theme	dynamic	691:697	arg1	spectroscopy					705:716	atomic force microscopy (AFM)-based dynamic force spectroscopy	655:716	atomic force microscopy (AFM)-based dynamic force spectroscopy	655:716	Here, we present a model mineral-SOM system consisting of mineral (mica)-nucleic acid (environmental DNA, eDNA)/protein (bovine serum albumin)/polysaccharide (alginate), and combine atomic force microscopy (AFM)-based dynamic force spectroscopy and PeakForce quantitative nanomechanical mapping using DNA-decorated tips.
31478650	5	7	from	observations	1248:1259	arg1	concentrations					1280:1293	relatively high concentrations	1264:1293	relatively high concentrations of divalent cation-containing acidic solutions	1264:1340	Consistent with the bond-strength measurements, protein, rather than polysaccharide, promotes mineral-bound DNA molecules by ex situ AFM deposition observations in relatively high concentrations of divalent cation-containing acidic solutions.
31478650	5	8	theme	divalent	1298:1305	arg1	solutions					1332:1340	divalent cation-containing acidic solutions	1298:1340	divalent cation-containing acidic solutions	1298:1340	Consistent with the bond-strength measurements, protein, rather than polysaccharide, promotes mineral-bound DNA molecules by ex situ AFM deposition observations in relatively high concentrations of divalent cation-containing acidic solutions.
31478650	5	9	with	Consistent	1100:1109	arg1	measurements					1134:1145	the bond-strength measurements	1116:1145	the bond-strength measurements	1116:1145	Consistent with the bond-strength measurements, protein, rather than polysaccharide, promotes mineral-bound DNA molecules by ex situ AFM deposition observations in relatively high concentrations of divalent cation-containing acidic solutions.
31478650	3	10	theme	force	699:703	arg1	spectroscopy					705:716	atomic force microscopy (AFM)-based dynamic force spectroscopy	655:716	atomic force microscopy (AFM)-based dynamic force spectroscopy	655:716	Here, we present a model mineral-SOM system consisting of mineral (mica)-nucleic acid (environmental DNA, eDNA)/protein (bovine serum albumin)/polysaccharide (alginate), and combine atomic force microscopy (AFM)-based dynamic force spectroscopy and PeakForce quantitative nanomechanical mapping using DNA-decorated tips.
31478650	6	11	theme	mineral-organic	1609:1623	arg1	associations					1625:1636	mineral-organic associations	1609:1636	mineral-organic associations	1609:1636	These molecular-scale determinations and nanoscale observations should substantially improve our understanding of how environmental factors influence the organic-mineral interfacial interactions through the synergy of collective noncovalent and/or covalent bonds in mineral-organic associations.
31478650	5	12	theme	cation-containing	1307:1323	arg1	solutions					1332:1340	divalent cation-containing acidic solutions	1298:1340	divalent cation-containing acidic solutions	1298:1340	Consistent with the bond-strength measurements, protein, rather than polysaccharide, promotes mineral-bound DNA molecules by ex situ AFM deposition observations in relatively high concentrations of divalent cation-containing acidic solutions.
31478650	0	13	from	Adsorption	73:82	arg1	Mica					108:111	Mica	108:111	Mica	108:111	Molecular-Scale Investigations Reveal Noncovalent Bonding Underlying the Adsorption of Environmental DNA on Mica.
31478650	4	14	theme	Single-molecule	794:808	arg1	binding					810:816	Single-molecule binding	794:816	Single-molecule binding	794:816	Single-molecule binding and adhesion force of eDNA to mineral and to mineral adsorbed by protein/polysaccharide reveal the noncovalent bonds and that systematically changing ion compositions, ionic strength, and pH result in significant differences in organic-organic and organic-mineral binding energies.
31478650	3	15	theme	-nucleic	545:552	arg1	acid					554:557	mineral (mica)-nucleic acid	531:557	mineral (mica)-nucleic acid (environmental DNA, eDNA)/protein (bovine serum albumin)/polysaccharide (alginate)	531:640	Here, we present a model mineral-SOM system consisting of mineral (mica)-nucleic acid (environmental DNA, eDNA)/protein (bovine serum albumin)/polysaccharide (alginate), and combine atomic force microscopy (AFM)-based dynamic force spectroscopy and PeakForce quantitative nanomechanical mapping using DNA-decorated tips.
31478650	1	16	theme	long-term	273:281	arg1	preservation					287:298	long-term SOM preservation	273:298	long-term SOM preservation	273:298	Mineral-soil organic matter (SOM including DNA, proteins, and polysaccharides) associations formed through various interactions, play a key role in regulating long-term SOM preservation.
31478650	1	17	theme	matter	135:140	arg1	associations					193:204	Mineral-soil organic matter (SOM including DNA, proteins, and polysaccharides) associations	114:204	Mineral-soil organic matter (SOM including DNA, proteins, and polysaccharides) associations	114:204	Mineral-soil organic matter (SOM including DNA, proteins, and polysaccharides) associations formed through various interactions, play a key role in regulating long-term SOM preservation.
31478650	1	18	dep	matter	135:140	arg1	SOM					143:145	SOM	143:145	SOM including DNA, proteins, and polysaccharides	143:190	Mineral-soil organic matter (SOM including DNA, proteins, and polysaccharides) associations formed through various interactions, play a key role in regulating long-term SOM preservation.
31478650	3	19	dep	/polysaccharide	615:629	arg1	albumin					607:613	bovine serum albumin	594:613	bovine serum albumin	594:613	Here, we present a model mineral-SOM system consisting of mineral (mica)-nucleic acid (environmental DNA, eDNA)/protein (bovine serum albumin)/polysaccharide (alginate), and combine atomic force microscopy (AFM)-based dynamic force spectroscopy and PeakForce quantitative nanomechanical mapping using DNA-decorated tips.
31478650	3	19	dep	/polysaccharide	615:629	arg1	alginate					632:639	alginate	632:639	alginate	632:639	Here, we present a model mineral-SOM system consisting of mineral (mica)-nucleic acid (environmental DNA, eDNA)/protein (bovine serum albumin)/polysaccharide (alginate), and combine atomic force microscopy (AFM)-based dynamic force spectroscopy and PeakForce quantitative nanomechanical mapping using DNA-decorated tips.
31478650	5	20	theme	acidic	1325:1330	arg1	solutions					1332:1340	divalent cation-containing acidic solutions	1298:1340	divalent cation-containing acidic solutions	1298:1340	Consistent with the bond-strength measurements, protein, rather than polysaccharide, promotes mineral-bound DNA molecules by ex situ AFM deposition observations in relatively high concentrations of divalent cation-containing acidic solutions.
31478650	3	21	theme	PeakForce	722:730	arg1	mapping					760:766	PeakForce quantitative nanomechanical mapping	722:766	PeakForce quantitative nanomechanical mapping using DNA-decorated tips	722:791	Here, we present a model mineral-SOM system consisting of mineral (mica)-nucleic acid (environmental DNA, eDNA)/protein (bovine serum albumin)/polysaccharide (alginate), and combine atomic force microscopy (AFM)-based dynamic force spectroscopy and PeakForce quantitative nanomechanical mapping using DNA-decorated tips.
31478650	6	22	theme	nanoscale	1384:1392	arg1	observations					1394:1405	nanoscale observations	1384:1405	nanoscale observations	1384:1405	These molecular-scale determinations and nanoscale observations should substantially improve our understanding of how environmental factors influence the organic-mineral interfacial interactions through the synergy of collective noncovalent and/or covalent bonds in mineral-organic associations.
31478650	3	23	theme	model	492:496	arg1	system					510:515	a model mineral-SOM system	490:515	a model mineral-SOM system consisting of mineral (mica)-nucleic acid (environmental DNA, eDNA)/protein (bovine serum albumin)/polysaccharide (alginate)	490:640	Here, we present a model mineral-SOM system consisting of mineral (mica)-nucleic acid (environmental DNA, eDNA)/protein (bovine serum albumin)/polysaccharide (alginate), and combine atomic force microscopy (AFM)-based dynamic force spectroscopy and PeakForce quantitative nanomechanical mapping using DNA-decorated tips.
31478650	3	24	theme	quantitative	732:743	arg1	mapping					760:766	PeakForce quantitative nanomechanical mapping	722:766	PeakForce quantitative nanomechanical mapping using DNA-decorated tips	722:791	Here, we present a model mineral-SOM system consisting of mineral (mica)-nucleic acid (environmental DNA, eDNA)/protein (bovine serum albumin)/polysaccharide (alginate), and combine atomic force microscopy (AFM)-based dynamic force spectroscopy and PeakForce quantitative nanomechanical mapping using DNA-decorated tips.
31478650	4	25	theme	significant	1019:1029	arg1	differences					1031:1041	significant differences	1019:1041	significant differences in organic-organic and organic-mineral binding energies	1019:1097	Single-molecule binding and adhesion force of eDNA to mineral and to mineral adsorbed by protein/polysaccharide reveal the noncovalent bonds and that systematically changing ion compositions, ionic strength, and pH result in significant differences in organic-organic and organic-mineral binding energies.
31478650	3	26	theme	bovine	594:599	arg1	albumin					607:613	bovine serum albumin	594:613	bovine serum albumin	594:613	Here, we present a model mineral-SOM system consisting of mineral (mica)-nucleic acid (environmental DNA, eDNA)/protein (bovine serum albumin)/polysaccharide (alginate), and combine atomic force microscopy (AFM)-based dynamic force spectroscopy and PeakForce quantitative nanomechanical mapping using DNA-decorated tips.
31478650	2	27	theme	relevant	436:443	arg1	solutions					445:453	environmentally relevant solutions	420:453	environmentally relevant solutions	420:453	The mechanisms underlying DNA-mineral and DNA-protein/polysaccharide interactions at nanometer and molecular scales in environmentally relevant solutions remain uncertain.
31478650	0	28	theme	Molecular-Scale	0:14	arg1	Investigations					16:29	Molecular-Scale Investigations	0:29	Molecular-Scale Investigations	0:29	Molecular-Scale Investigations Reveal Noncovalent Bonding Underlying the Adsorption of Environmental DNA on Mica.
31478650	1	29	theme	SOM	283:285	arg1	preservation					287:298	long-term SOM preservation	273:298	long-term SOM preservation	273:298	Mineral-soil organic matter (SOM including DNA, proteins, and polysaccharides) associations formed through various interactions, play a key role in regulating long-term SOM preservation.
31478650	3	30	theme	serum	601:605	arg1	albumin					607:613	bovine serum albumin	594:613	bovine serum albumin	594:613	Here, we present a model mineral-SOM system consisting of mineral (mica)-nucleic acid (environmental DNA, eDNA)/protein (bovine serum albumin)/polysaccharide (alginate), and combine atomic force microscopy (AFM)-based dynamic force spectroscopy and PeakForce quantitative nanomechanical mapping using DNA-decorated tips.
31478650	0	31	theme	Noncovalent	38:48	arg1	Bonding					50:56	Noncovalent Bonding	38:56	Noncovalent Bonding Underlying the Adsorption of Environmental DNA on Mica	38:111	Molecular-Scale Investigations Reveal Noncovalent Bonding Underlying the Adsorption of Environmental DNA on Mica.
31478650	5	32	dep	ex	1225:1226	arg1	situ					1228:1231	situ	1228:1231	situ	1228:1231	Consistent with the bond-strength measurements, protein, rather than polysaccharide, promotes mineral-bound DNA molecules by ex situ AFM deposition observations in relatively high concentrations of divalent cation-containing acidic solutions.
31478650	6	33	theme	organic-mineral	1497:1511	arg1	interactions					1525:1536	the organic-mineral interfacial interactions	1493:1536	the organic-mineral interfacial interactions	1493:1536	These molecular-scale determinations and nanoscale observations should substantially improve our understanding of how environmental factors influence the organic-mineral interfacial interactions through the synergy of collective noncovalent and/or covalent bonds in mineral-organic associations.
31478650	2	34	from	scales	410:415	arg1	solutions					445:453	environmentally relevant solutions	420:453	environmentally relevant solutions	420:453	The mechanisms underlying DNA-mineral and DNA-protein/polysaccharide interactions at nanometer and molecular scales in environmentally relevant solutions remain uncertain.
31478650	1	35	theme	Mineral-soil	114:125	arg1	matter					135:140	Mineral-soil organic matter	114:140	Mineral-soil organic matter (SOM including DNA, proteins, and polysaccharides) associations	114:204	Mineral-soil organic matter (SOM including DNA, proteins, and polysaccharides) associations formed through various interactions, play a key role in regulating long-term SOM preservation.
31478650	6	36	theme	molecular-scale	1349:1363	arg1	determinations					1365:1378	These molecular-scale determinations	1343:1378	These molecular-scale determinations	1343:1378	These molecular-scale determinations and nanoscale observations should substantially improve our understanding of how environmental factors influence the organic-mineral interfacial interactions through the synergy of collective noncovalent and/or covalent bonds in mineral-organic associations.
31478650	2	37	theme	DNA-mineral	327:337	arg1	interactions					370:381	DNA-mineral and DNA-protein/polysaccharide interactions	327:381	DNA-mineral and DNA-protein/polysaccharide interactions	327:381	The mechanisms underlying DNA-mineral and DNA-protein/polysaccharide interactions at nanometer and molecular scales in environmentally relevant solutions remain uncertain.
31478650	5	38	theme	high	1275:1278	arg1	concentrations					1280:1293	relatively high concentrations	1264:1293	relatively high concentrations of divalent cation-containing acidic solutions	1264:1340	Consistent with the bond-strength measurements, protein, rather than polysaccharide, promotes mineral-bound DNA molecules by ex situ AFM deposition observations in relatively high concentrations of divalent cation-containing acidic solutions.
31478650	4	39	theme	adhesion	822:829	arg1	force					831:835	adhesion force	822:835	adhesion force	822:835	Single-molecule binding and adhesion force of eDNA to mineral and to mineral adsorbed by protein/polysaccharide reveal the noncovalent bonds and that systematically changing ion compositions, ionic strength, and pH result in significant differences in organic-organic and organic-mineral binding energies.
31478650	3	40	theme	force	662:666	arg1	AFM					680:682	AFM	680:682	AFM	680:682	Here, we present a model mineral-SOM system consisting of mineral (mica)-nucleic acid (environmental DNA, eDNA)/protein (bovine serum albumin)/polysaccharide (alginate), and combine atomic force microscopy (AFM)-based dynamic force spectroscopy and PeakForce quantitative nanomechanical mapping using DNA-decorated tips.
31478650	3	40	theme	force	662:666	arg1	microscopy					668:677	atomic force microscopy	655:677	atomic force microscopy (AFM)	655:683	Here, we present a model mineral-SOM system consisting of mineral (mica)-nucleic acid (environmental DNA, eDNA)/protein (bovine serum albumin)/polysaccharide (alginate), and combine atomic force microscopy (AFM)-based dynamic force spectroscopy and PeakForce quantitative nanomechanical mapping using DNA-decorated tips.
31478650	1	41	theme	organic	127:133	arg1	matter					135:140	Mineral-soil organic matter	114:140	Mineral-soil organic matter (SOM including DNA, proteins, and polysaccharides) associations	114:204	Mineral-soil organic matter (SOM including DNA, proteins, and polysaccharides) associations formed through various interactions, play a key role in regulating long-term SOM preservation.
31478650	3	42	theme	DNA-decorated	774:786	arg1	tips					788:791	DNA-decorated tips	774:791	DNA-decorated tips	774:791	Here, we present a model mineral-SOM system consisting of mineral (mica)-nucleic acid (environmental DNA, eDNA)/protein (bovine serum albumin)/polysaccharide (alginate), and combine atomic force microscopy (AFM)-based dynamic force spectroscopy and PeakForce quantitative nanomechanical mapping using DNA-decorated tips.
31478650	1	43	theme	various	221:227	arg1	interactions					229:240	various interactions	221:240	various interactions	221:240	Mineral-soil organic matter (SOM including DNA, proteins, and polysaccharides) associations formed through various interactions, play a key role in regulating long-term SOM preservation.
31478650	3	44	theme	/polysaccharide	615:629	arg1	acid					554:557	mineral (mica)-nucleic acid	531:557	mineral (mica)-nucleic acid (environmental DNA, eDNA)/protein (bovine serum albumin)/polysaccharide (alginate)	531:640	Here, we present a model mineral-SOM system consisting of mineral (mica)-nucleic acid (environmental DNA, eDNA)/protein (bovine serum albumin)/polysaccharide (alginate), and combine atomic force microscopy (AFM)-based dynamic force spectroscopy and PeakForce quantitative nanomechanical mapping using DNA-decorated tips.
31478650	3	45	theme	mineral-SOM	498:508	arg1	system					510:515	a model mineral-SOM system	490:515	a model mineral-SOM system consisting of mineral (mica)-nucleic acid (environmental DNA, eDNA)/protein (bovine serum albumin)/polysaccharide (alginate)	490:640	Here, we present a model mineral-SOM system consisting of mineral (mica)-nucleic acid (environmental DNA, eDNA)/protein (bovine serum albumin)/polysaccharide (alginate), and combine atomic force microscopy (AFM)-based dynamic force spectroscopy and PeakForce quantitative nanomechanical mapping using DNA-decorated tips.
31478650	4	46	theme	organic-organic	1046:1060	arg1	energies					1090:1097	organic-organic and organic-mineral binding energies	1046:1097	organic-organic and organic-mineral binding energies	1046:1097	Single-molecule binding and adhesion force of eDNA to mineral and to mineral adsorbed by protein/polysaccharide reveal the noncovalent bonds and that systematically changing ion compositions, ionic strength, and pH result in significant differences in organic-organic and organic-mineral binding energies.
31478650	4	47	theme	ionic	986:990	arg1	strength					992:999	ionic strength	986:999	ionic strength	986:999	Single-molecule binding and adhesion force of eDNA to mineral and to mineral adsorbed by protein/polysaccharide reveal the noncovalent bonds and that systematically changing ion compositions, ionic strength, and pH result in significant differences in organic-organic and organic-mineral binding energies.
31478650	6	48	theme	interfacial	1513:1523	arg1	interactions					1525:1536	the organic-mineral interfacial interactions	1493:1536	the organic-mineral interfacial interactions	1493:1536	These molecular-scale determinations and nanoscale observations should substantially improve our understanding of how environmental factors influence the organic-mineral interfacial interactions through the synergy of collective noncovalent and/or covalent bonds in mineral-organic associations.
31478650	4	49	theme	binding	1082:1088	arg1	energies					1090:1097	organic-organic and organic-mineral binding energies	1046:1097	organic-organic and organic-mineral binding energies	1046:1097	Single-molecule binding and adhesion force of eDNA to mineral and to mineral adsorbed by protein/polysaccharide reveal the noncovalent bonds and that systematically changing ion compositions, ionic strength, and pH result in significant differences in organic-organic and organic-mineral binding energies.
31478650	5	50	theme	AFM	1233:1235	arg1	observations					1248:1259	ex situ AFM deposition observations	1225:1259	ex situ AFM deposition observations in relatively high concentrations of divalent cation-containing acidic solutions	1225:1340	Consistent with the bond-strength measurements, protein, rather than polysaccharide, promotes mineral-bound DNA molecules by ex situ AFM deposition observations in relatively high concentrations of divalent cation-containing acidic solutions.
31478650	6	51	theme	environmental	1461:1473	arg1	factors					1475:1481	environmental factors	1461:1481	environmental factors	1461:1481	These molecular-scale determinations and nanoscale observations should substantially improve our understanding of how environmental factors influence the organic-mineral interfacial interactions through the synergy of collective noncovalent and/or covalent bonds in mineral-organic associations.
31478650	3	52	theme	atomic	655:660	arg1	AFM					680:682	AFM	680:682	AFM	680:682	Here, we present a model mineral-SOM system consisting of mineral (mica)-nucleic acid (environmental DNA, eDNA)/protein (bovine serum albumin)/polysaccharide (alginate), and combine atomic force microscopy (AFM)-based dynamic force spectroscopy and PeakForce quantitative nanomechanical mapping using DNA-decorated tips.
31478650	3	52	theme	atomic	655:660	arg1	microscopy					668:677	atomic force microscopy	655:677	atomic force microscopy (AFM)	655:683	Here, we present a model mineral-SOM system consisting of mineral (mica)-nucleic acid (environmental DNA, eDNA)/protein (bovine serum albumin)/polysaccharide (alginate), and combine atomic force microscopy (AFM)-based dynamic force spectroscopy and PeakForce quantitative nanomechanical mapping using DNA-decorated tips.
31478650	2	53	theme	nanometer	386:394	arg1	scales					410:415	nanometer and molecular scales	386:415	nanometer and molecular scales in environmentally relevant solutions	386:453	The mechanisms underlying DNA-mineral and DNA-protein/polysaccharide interactions at nanometer and molecular scales in environmentally relevant solutions remain uncertain.
31478650	3	54	theme	mica	540:543	arg1	acid					554:557	mineral (mica)-nucleic acid	531:557	mineral (mica)-nucleic acid (environmental DNA, eDNA)/protein (bovine serum albumin)/polysaccharide (alginate)	531:640	Here, we present a model mineral-SOM system consisting of mineral (mica)-nucleic acid (environmental DNA, eDNA)/protein (bovine serum albumin)/polysaccharide (alginate), and combine atomic force microscopy (AFM)-based dynamic force spectroscopy and PeakForce quantitative nanomechanical mapping using DNA-decorated tips.
31478650	4	55	theme	organic-mineral	1066:1080	arg1	energies					1090:1097	organic-organic and organic-mineral binding energies	1046:1097	organic-organic and organic-mineral binding energies	1046:1097	Single-molecule binding and adhesion force of eDNA to mineral and to mineral adsorbed by protein/polysaccharide reveal the noncovalent bonds and that systematically changing ion compositions, ionic strength, and pH result in significant differences in organic-organic and organic-mineral binding energies.
31478650	5	56	theme	deposition	1237:1246	arg1	observations					1248:1259	ex situ AFM deposition observations	1225:1259	ex situ AFM deposition observations in relatively high concentrations of divalent cation-containing acidic solutions	1225:1340	Consistent with the bond-strength measurements, protein, rather than polysaccharide, promotes mineral-bound DNA molecules by ex situ AFM deposition observations in relatively high concentrations of divalent cation-containing acidic solutions.
31478650	5	57	theme	bond-strength	1120:1132	arg1	measurements					1134:1145	the bond-strength measurements	1116:1145	the bond-strength measurements	1116:1145	Consistent with the bond-strength measurements, protein, rather than polysaccharide, promotes mineral-bound DNA molecules by ex situ AFM deposition observations in relatively high concentrations of divalent cation-containing acidic solutions.
31478650	3	58	dep	acid	554:557	arg1	eDNA					579:582	eDNA	579:582	eDNA	579:582	Here, we present a model mineral-SOM system consisting of mineral (mica)-nucleic acid (environmental DNA, eDNA)/protein (bovine serum albumin)/polysaccharide (alginate), and combine atomic force microscopy (AFM)-based dynamic force spectroscopy and PeakForce quantitative nanomechanical mapping using DNA-decorated tips.
31478650	3	58	dep	acid	554:557	arg1	DNA					574:576	environmental DNA	560:576	environmental DNA	560:576	Here, we present a model mineral-SOM system consisting of mineral (mica)-nucleic acid (environmental DNA, eDNA)/protein (bovine serum albumin)/polysaccharide (alginate), and combine atomic force microscopy (AFM)-based dynamic force spectroscopy and PeakForce quantitative nanomechanical mapping using DNA-decorated tips.
31478650	6	59	theme	bonds	1600:1604	arg1	synergy					1550:1556	the synergy	1546:1556	the synergy of collective noncovalent and/or covalent bonds in mineral-organic associations	1546:1636	These molecular-scale determinations and nanoscale observations should substantially improve our understanding of how environmental factors influence the organic-mineral interfacial interactions through the synergy of collective noncovalent and/or covalent bonds in mineral-organic associations.
31478650	4	60	theme	ion	968:970	arg1	compositions					972:983	systematically changing ion compositions	944:983	systematically changing ion compositions	944:983	Single-molecule binding and adhesion force of eDNA to mineral and to mineral adsorbed by protein/polysaccharide reveal the noncovalent bonds and that systematically changing ion compositions, ionic strength, and pH result in significant differences in organic-organic and organic-mineral binding energies.
31478650	3	61	theme	-based	684:689	arg1	spectroscopy					705:716	atomic force microscopy (AFM)-based dynamic force spectroscopy	655:716	atomic force microscopy (AFM)-based dynamic force spectroscopy	655:716	Here, we present a model mineral-SOM system consisting of mineral (mica)-nucleic acid (environmental DNA, eDNA)/protein (bovine serum albumin)/polysaccharide (alginate), and combine atomic force microscopy (AFM)-based dynamic force spectroscopy and PeakForce quantitative nanomechanical mapping using DNA-decorated tips.
31478650	3	62	theme	mineral	531:537	arg1	acid					554:557	mineral (mica)-nucleic acid	531:557	mineral (mica)-nucleic acid (environmental DNA, eDNA)/protein (bovine serum albumin)/polysaccharide (alginate)	531:640	Here, we present a model mineral-SOM system consisting of mineral (mica)-nucleic acid (environmental DNA, eDNA)/protein (bovine serum albumin)/polysaccharide (alginate), and combine atomic force microscopy (AFM)-based dynamic force spectroscopy and PeakForce quantitative nanomechanical mapping using DNA-decorated tips.
31478650	5	63	theme	solutions	1332:1340	arg1	concentrations					1280:1293	relatively high concentrations	1264:1293	relatively high concentrations of divalent cation-containing acidic solutions	1264:1340	Consistent with the bond-strength measurements, protein, rather than polysaccharide, promotes mineral-bound DNA molecules by ex situ AFM deposition observations in relatively high concentrations of divalent cation-containing acidic solutions.
31478650	4	64	theme	changing	959:966	arg1	compositions					972:983	systematically changing ion compositions	944:983	systematically changing ion compositions	944:983	Single-molecule binding and adhesion force of eDNA to mineral and to mineral adsorbed by protein/polysaccharide reveal the noncovalent bonds and that systematically changing ion compositions, ionic strength, and pH result in significant differences in organic-organic and organic-mineral binding energies.
31478650	6	65	theme	noncovalent	1572:1582	arg1	synergy					1550:1556	the synergy	1546:1556	the synergy of collective noncovalent and/or covalent bonds in mineral-organic associations	1546:1636	These molecular-scale determinations and nanoscale observations should substantially improve our understanding of how environmental factors influence the organic-mineral interfacial interactions through the synergy of collective noncovalent and/or covalent bonds in mineral-organic associations.
31478650	2	66	theme	DNA-protein/polysaccharide	343:368	arg1	interactions					370:381	DNA-mineral and DNA-protein/polysaccharide interactions	327:381	DNA-mineral and DNA-protein/polysaccharide interactions	327:381	The mechanisms underlying DNA-mineral and DNA-protein/polysaccharide interactions at nanometer and molecular scales in environmentally relevant solutions remain uncertain.
31478650	4	67	from	differences	1031:1041	arg1	energies					1090:1097	organic-organic and organic-mineral binding energies	1046:1097	organic-organic and organic-mineral binding energies	1046:1097	Single-molecule binding and adhesion force of eDNA to mineral and to mineral adsorbed by protein/polysaccharide reveal the noncovalent bonds and that systematically changing ion compositions, ionic strength, and pH result in significant differences in organic-organic and organic-mineral binding energies.
31478650	0	68	theme	Environmental	87:99	arg1	DNA					101:103	Environmental DNA	87:103	Environmental DNA	87:103	Molecular-Scale Investigations Reveal Noncovalent Bonding Underlying the Adsorption of Environmental DNA on Mica.
31478650	5	69	theme	ex	1225:1226	arg1	observations					1248:1259	ex situ AFM deposition observations	1225:1259	ex situ AFM deposition observations in relatively high concentrations of divalent cation-containing acidic solutions	1225:1340	Consistent with the bond-strength measurements, protein, rather than polysaccharide, promotes mineral-bound DNA molecules by ex situ AFM deposition observations in relatively high concentrations of divalent cation-containing acidic solutions.
31478650	6	70	theme	covalent	1591:1598	arg1	bonds					1600:1604	collective noncovalent and/or covalent bonds	1561:1604	bonds	1600:1604	These molecular-scale determinations and nanoscale observations should substantially improve our understanding of how environmental factors influence the organic-mineral interfacial interactions through the synergy of collective noncovalent and/or covalent bonds in mineral-organic associations.
31478650	5	71	theme	DNA	1208:1210	arg1	molecules					1212:1220	mineral-bound DNA molecules	1194:1220	mineral-bound DNA molecules	1194:1220	Consistent with the bond-strength measurements, protein, rather than polysaccharide, promotes mineral-bound DNA molecules by ex situ AFM deposition observations in relatively high concentrations of divalent cation-containing acidic solutions.
31478650	4	72	theme	eDNA	840:843	arg1	binding					810:816	Single-molecule binding	794:816	Single-molecule binding	794:816	Single-molecule binding and adhesion force of eDNA to mineral and to mineral adsorbed by protein/polysaccharide reveal the noncovalent bonds and that systematically changing ion compositions, ionic strength, and pH result in significant differences in organic-organic and organic-mineral binding energies.
31478650	4	72	theme	eDNA	840:843	arg1	force					831:835	adhesion force	822:835	adhesion force	822:835	Single-molecule binding and adhesion force of eDNA to mineral and to mineral adsorbed by protein/polysaccharide reveal the noncovalent bonds and that systematically changing ion compositions, ionic strength, and pH result in significant differences in organic-organic and organic-mineral binding energies.
31478650	3	73	theme	environmental	560:572	arg1	eDNA					579:582	eDNA	579:582	eDNA	579:582	Here, we present a model mineral-SOM system consisting of mineral (mica)-nucleic acid (environmental DNA, eDNA)/protein (bovine serum albumin)/polysaccharide (alginate), and combine atomic force microscopy (AFM)-based dynamic force spectroscopy and PeakForce quantitative nanomechanical mapping using DNA-decorated tips.
31478650	3	73	theme	environmental	560:572	arg1	DNA					574:576	environmental DNA	560:576	environmental DNA	560:576	Here, we present a model mineral-SOM system consisting of mineral (mica)-nucleic acid (environmental DNA, eDNA)/protein (bovine serum albumin)/polysaccharide (alginate), and combine atomic force microscopy (AFM)-based dynamic force spectroscopy and PeakForce quantitative nanomechanical mapping using DNA-decorated tips.
31478650	1	74	theme	key	250:252	arg1	role					254:257	a key role	248:257	a key role	248:257	Mineral-soil organic matter (SOM including DNA, proteins, and polysaccharides) associations formed through various interactions, play a key role in regulating long-term SOM preservation.
31357563	0	0	theme	paniculata	90:99	arg1	Parts					68:72	the Aerial Parts	57:72	the Aerial Parts of Andrographis paniculata	57:99	Diterpenoid Lactones with Anti-Inflammatory Effects from the Aerial Parts of Andrographis paniculata.
31357563	5	1	theme	responsible	744:754	arg1	lactones					735:742	diterpenoid lactones	723:742	diterpenoid lactones responsible for the anti-inflammatory activities	723:791	Pharmacophores of diterpenoid lactones responsible for the anti-inflammatory activities were revealed based on the quantitative structure-activity relationship (QSAR) models.
31357563	3	2	from	AP	444:445	arg1	composition					339:349	systematic chemical composition	319:349	systematic chemical composition	319:349	However, systematic chemical composition and biological activities, as well as key pharmacophores, of these diterpenoid lactones from AP have not yet been clearly understood.
31357563	3	2	from	AP	444:445	arg1	activities					366:375	biological activities	355:375	biological activities	355:375	However, systematic chemical composition and biological activities, as well as key pharmacophores, of these diterpenoid lactones from AP have not yet been clearly understood.
31357563	3	2	from	AP	444:445	arg1	pharmacophores					393:406	key pharmacophores	389:406	key pharmacophores	389:406	However, systematic chemical composition and biological activities, as well as key pharmacophores, of these diterpenoid lactones from AP have not yet been clearly understood.
31357563	4	3	theme	RAW	670:672	arg1	cells					680:684	LPS-induced RAW 274.7 cells	658:684	LPS-induced RAW 274.7 cells examined by ELISA	658:702	In this study, 17 diterpenoid lactones, including 2 new compounds, were identified by spectroscopic methods, and most of them attenuated the generation of TNF-α and IL-6 in LPS-induced RAW 274.7 cells examined by ELISA.
31357563	0	4	theme	Andrographis	77:88	arg1	paniculata					90:99	Andrographis paniculata	77:99	Andrographis paniculata	77:99	Diterpenoid Lactones with Anti-Inflammatory Effects from the Aerial Parts of Andrographis paniculata.
31357563	4	5	theme	LPS-induced	658:668	arg1	cells					680:684	LPS-induced RAW 274.7 cells	658:684	LPS-induced RAW 274.7 cells examined by ELISA	658:702	In this study, 17 diterpenoid lactones, including 2 new compounds, were identified by spectroscopic methods, and most of them attenuated the generation of TNF-α and IL-6 in LPS-induced RAW 274.7 cells examined by ELISA.
31357563	6	6	from	inhibition	1031:1040	arg1	cytoplasm					1081:1089	cytoplasm	1081:1089	cytoplasm	1081:1089	Moreover, new compounds (AP-1 and AP-4) exerted anti-inflammatory activity in LPS microinjection-induced zebrafish, which might be correlated with the inhibition of the translocation of NF-κB p65 from cytoplasm to nucleus.
31357563	4	7	theme	spectroscopic	571:583	arg1	methods					585:591	spectroscopic methods	571:591	spectroscopic methods	571:591	In this study, 17 diterpenoid lactones, including 2 new compounds, were identified by spectroscopic methods, and most of them attenuated the generation of TNF-α and IL-6 in LPS-induced RAW 274.7 cells examined by ELISA.
31357563	7	8	theme	anti-inflammatory	1221:1237	arg1	properties					1239:1248	anti-inflammatory properties	1221:1248	anti-inflammatory properties in medical chemistry	1221:1269	Our study provides guidelines for future structure modification and rational drug design of diterpenoid lactones with anti-inflammatory properties in medical chemistry.
31357563	6	9	from	cytoplasm	1081:1089	arg1	translocation					1049:1061	the translocation	1045:1061	the translocation of NF-κB p65 from cytoplasm to nucleus	1045:1100	Moreover, new compounds (AP-1 and AP-4) exerted anti-inflammatory activity in LPS microinjection-induced zebrafish, which might be correlated with the inhibition of the translocation of NF-κB p65 from cytoplasm to nucleus.
31357563	6	9	from	cytoplasm	1081:1089	arg1	inhibition					1031:1040	the inhibition	1027:1040	the inhibition of the translocation of NF-κB p65 from cytoplasm to nucleus	1027:1100	Moreover, new compounds (AP-1 and AP-4) exerted anti-inflammatory activity in LPS microinjection-induced zebrafish, which might be correlated with the inhibition of the translocation of NF-κB p65 from cytoplasm to nucleus.
31357563	2	10	theme	anti-inflammatory	274:290	arg1	components					292:301	the main anti-inflammatory components	265:301	the main anti-inflammatory components of AP	265:307	Diterpenoid lactones are the main anti-inflammatory components of AP.
31357563	2	10	theme	anti-inflammatory	274:290	arg1	lactones					252:259	Diterpenoid lactones	240:259	Diterpenoid lactones	240:259	Diterpenoid lactones are the main anti-inflammatory components of AP.
31357563	7	11	theme	diterpenoid	1195:1205	arg1	lactones					1207:1214	diterpenoid lactones	1195:1214	diterpenoid lactones	1195:1214	Our study provides guidelines for future structure modification and rational drug design of diterpenoid lactones with anti-inflammatory properties in medical chemistry.
31357563	6	12	theme	new	890:892	arg1	AP-1					905:908	AP-1	905:908	AP-1	905:908	Moreover, new compounds (AP-1 and AP-4) exerted anti-inflammatory activity in LPS microinjection-induced zebrafish, which might be correlated with the inhibition of the translocation of NF-κB p65 from cytoplasm to nucleus.
31357563	6	12	theme	new	890:892	arg1	compounds					894:902	new compounds	890:902	new compounds (AP-1 and AP-4)	890:918	Moreover, new compounds (AP-1 and AP-4) exerted anti-inflammatory activity in LPS microinjection-induced zebrafish, which might be correlated with the inhibition of the translocation of NF-κB p65 from cytoplasm to nucleus.
31357563	6	12	theme	new	890:892	arg1	AP-4					914:917	AP-4	914:917	AP-4	914:917	Moreover, new compounds (AP-1 and AP-4) exerted anti-inflammatory activity in LPS microinjection-induced zebrafish, which might be correlated with the inhibition of the translocation of NF-κB p65 from cytoplasm to nucleus.
31357563	6	13	theme	translocation	1049:1061	arg1	inhibition					1031:1040	the inhibition	1027:1040	the inhibition of the translocation of NF-κB p65 from cytoplasm to nucleus	1027:1100	Moreover, new compounds (AP-1 and AP-4) exerted anti-inflammatory activity in LPS microinjection-induced zebrafish, which might be correlated with the inhibition of the translocation of NF-κB p65 from cytoplasm to nucleus.
31357563	1	14	used	used	147:150	arg2	AP					127:128	AP	127:128	AP	127:128	Andrographis paniculata (AP) has been widely used in China for centuries to treat various diseases, and especially to treat inflammation.
31357563	1	14	used	used	147:150	arg2	paniculata					115:124	Andrographis paniculata	102:124	Andrographis paniculata (AP)	102:129	Andrographis paniculata (AP) has been widely used in China for centuries to treat various diseases, and especially to treat inflammation.
31357563	6	15	theme	p65	1072:1074	arg1	translocation					1049:1061	the translocation	1045:1061	the translocation of NF-κB p65 from cytoplasm to nucleus	1045:1100	Moreover, new compounds (AP-1 and AP-4) exerted anti-inflammatory activity in LPS microinjection-induced zebrafish, which might be correlated with the inhibition of the translocation of NF-κB p65 from cytoplasm to nucleus.
31357563	3	16	theme	biological	355:364	arg1	activities					366:375	biological activities	355:375	biological activities	355:375	However, systematic chemical composition and biological activities, as well as key pharmacophores, of these diterpenoid lactones from AP have not yet been clearly understood.
31357563	7	17	theme	future	1137:1142	arg1	modification					1154:1165	future structure modification	1137:1165	future structure modification	1137:1165	Our study provides guidelines for future structure modification and rational drug design of diterpenoid lactones with anti-inflammatory properties in medical chemistry.
31357563	3	18	theme	systematic	319:328	arg1	composition					339:349	systematic chemical composition	319:349	systematic chemical composition	319:349	However, systematic chemical composition and biological activities, as well as key pharmacophores, of these diterpenoid lactones from AP have not yet been clearly understood.
31357563	5	19	theme	diterpenoid	723:733	arg1	lactones					735:742	diterpenoid lactones	723:742	diterpenoid lactones responsible for the anti-inflammatory activities	723:791	Pharmacophores of diterpenoid lactones responsible for the anti-inflammatory activities were revealed based on the quantitative structure-activity relationship (QSAR) models.
31357563	4	20	from	generation	626:635	arg1	cells					680:684	LPS-induced RAW 274.7 cells	658:684	LPS-induced RAW 274.7 cells examined by ELISA	658:702	In this study, 17 diterpenoid lactones, including 2 new compounds, were identified by spectroscopic methods, and most of them attenuated the generation of TNF-α and IL-6 in LPS-induced RAW 274.7 cells examined by ELISA.
31357563	3	21	theme	diterpenoid	418:428	arg1	lactones					430:437	these diterpenoid lactones	412:437	these diterpenoid lactones	412:437	However, systematic chemical composition and biological activities, as well as key pharmacophores, of these diterpenoid lactones from AP have not yet been clearly understood.
31357563	3	22	theme	key	389:391	arg1	pharmacophores					393:406	key pharmacophores	389:406	key pharmacophores	389:406	However, systematic chemical composition and biological activities, as well as key pharmacophores, of these diterpenoid lactones from AP have not yet been clearly understood.
31357563	4	23	theme	new	537:539	arg1	compounds					541:549	2 new compounds	535:549	2 new compounds	535:549	In this study, 17 diterpenoid lactones, including 2 new compounds, were identified by spectroscopic methods, and most of them attenuated the generation of TNF-α and IL-6 in LPS-induced RAW 274.7 cells examined by ELISA.
31357563	1	24	theme	Andrographis	102:113	arg1	AP					127:128	AP	127:128	AP	127:128	Andrographis paniculata (AP) has been widely used in China for centuries to treat various diseases, and especially to treat inflammation.
31357563	1	24	theme	Andrographis	102:113	arg1	paniculata					115:124	Andrographis paniculata	102:124	Andrographis paniculata (AP)	102:129	Andrographis paniculata (AP) has been widely used in China for centuries to treat various diseases, and especially to treat inflammation.
31357563	3	25	theme	lactones	430:437	arg1	composition					339:349	systematic chemical composition	319:349	systematic chemical composition	319:349	However, systematic chemical composition and biological activities, as well as key pharmacophores, of these diterpenoid lactones from AP have not yet been clearly understood.
31357563	3	25	theme	lactones	430:437	arg1	activities					366:375	biological activities	355:375	biological activities	355:375	However, systematic chemical composition and biological activities, as well as key pharmacophores, of these diterpenoid lactones from AP have not yet been clearly understood.
31357563	3	25	theme	lactones	430:437	arg1	pharmacophores					393:406	key pharmacophores	389:406	key pharmacophores	389:406	However, systematic chemical composition and biological activities, as well as key pharmacophores, of these diterpenoid lactones from AP have not yet been clearly understood.
31357563	0	26	theme	Anti-Inflammatory	26:42	arg1	Effects					44:50	Anti-Inflammatory Effects	26:50	Anti-Inflammatory Effects from the Aerial Parts of Andrographis paniculata	26:99	Diterpenoid Lactones with Anti-Inflammatory Effects from the Aerial Parts of Andrographis paniculata.
31357563	6	27	theme	microinjection-induced	962:983	arg1	zebrafish					985:993	LPS microinjection-induced zebrafish	958:993	LPS microinjection-induced zebrafish	958:993	Moreover, new compounds (AP-1 and AP-4) exerted anti-inflammatory activity in LPS microinjection-induced zebrafish, which might be correlated with the inhibition of the translocation of NF-κB p65 from cytoplasm to nucleus.
31357563	2	28	theme	AP	306:307	arg1	components					292:301	the main anti-inflammatory components	265:301	the main anti-inflammatory components of AP	265:307	Diterpenoid lactones are the main anti-inflammatory components of AP.
31357563	2	28	theme	AP	306:307	arg1	lactones					252:259	Diterpenoid lactones	240:259	Diterpenoid lactones	240:259	Diterpenoid lactones are the main anti-inflammatory components of AP.
31357563	1	29	theme	various	184:190	arg1	diseases					192:199	various diseases	184:199	various diseases	184:199	Andrographis paniculata (AP) has been widely used in China for centuries to treat various diseases, and especially to treat inflammation.
31357563	7	30	mod	modification	1154:1165	arg3	structure					1144:1152	future structure modification	1137:1165	future structure modification	1137:1165	Our study provides guidelines for future structure modification and rational drug design of diterpenoid lactones with anti-inflammatory properties in medical chemistry.
31357563	7	30	mod	modification	1154:1165	arg1	lactones					1207:1214	diterpenoid lactones	1195:1214	diterpenoid lactones	1195:1214	Our study provides guidelines for future structure modification and rational drug design of diterpenoid lactones with anti-inflammatory properties in medical chemistry.
31357563	6	31	theme	LPS	958:960	arg1	zebrafish					985:993	LPS microinjection-induced zebrafish	958:993	LPS microinjection-induced zebrafish	958:993	Moreover, new compounds (AP-1 and AP-4) exerted anti-inflammatory activity in LPS microinjection-induced zebrafish, which might be correlated with the inhibition of the translocation of NF-κB p65 from cytoplasm to nucleus.
31357563	2	32	theme	main	269:272	arg1	components					292:301	the main anti-inflammatory components	265:301	the main anti-inflammatory components of AP	265:307	Diterpenoid lactones are the main anti-inflammatory components of AP.
31357563	2	32	theme	main	269:272	arg1	lactones					252:259	Diterpenoid lactones	240:259	Diterpenoid lactones	240:259	Diterpenoid lactones are the main anti-inflammatory components of AP.
31357563	5	33	theme	structure-activity	833:850	arg1	relationship					852:863	quantitative structure-activity relationship	820:863	the quantitative structure-activity relationship (QSAR) models	816:877	Pharmacophores of diterpenoid lactones responsible for the anti-inflammatory activities were revealed based on the quantitative structure-activity relationship (QSAR) models.
31357563	5	33	theme	structure-activity	833:850	arg1	QSAR					866:869	QSAR	866:869	QSAR	866:869	Pharmacophores of diterpenoid lactones responsible for the anti-inflammatory activities were revealed based on the quantitative structure-activity relationship (QSAR) models.
31357563	4	34	theme	TNF-α	640:644	arg1	generation					626:635	the generation	622:635	the generation of TNF-α and IL-6 in LPS-induced RAW 274.7 cells examined by ELISA	622:702	In this study, 17 diterpenoid lactones, including 2 new compounds, were identified by spectroscopic methods, and most of them attenuated the generation of TNF-α and IL-6 in LPS-induced RAW 274.7 cells examined by ELISA.
31357563	5	35	theme	relationship	852:863	arg1	models					872:877	the quantitative structure-activity relationship (QSAR) models	816:877	the quantitative structure-activity relationship (QSAR) models	816:877	Pharmacophores of diterpenoid lactones responsible for the anti-inflammatory activities were revealed based on the quantitative structure-activity relationship (QSAR) models.
31357563	5	36	theme	anti-inflammatory	764:780	arg1	activities					782:791	the anti-inflammatory activities	760:791	the anti-inflammatory activities	760:791	Pharmacophores of diterpenoid lactones responsible for the anti-inflammatory activities were revealed based on the quantitative structure-activity relationship (QSAR) models.
31357563	7	37	theme	rational	1171:1178	arg1	design					1185:1190	rational drug design	1171:1190	rational drug design	1171:1190	Our study provides guidelines for future structure modification and rational drug design of diterpenoid lactones with anti-inflammatory properties in medical chemistry.
31357563	7	38	theme	lactones	1207:1214	arg1	modification					1154:1165	future structure modification	1137:1165	future structure modification	1137:1165	Our study provides guidelines for future structure modification and rational drug design of diterpenoid lactones with anti-inflammatory properties in medical chemistry.
31357563	7	38	theme	lactones	1207:1214	arg1	design					1185:1190	rational drug design	1171:1190	rational drug design	1171:1190	Our study provides guidelines for future structure modification and rational drug design of diterpenoid lactones with anti-inflammatory properties in medical chemistry.
31357563	0	39	theme	Aerial	61:66	arg1	Parts					68:72	the Aerial Parts	57:72	the Aerial Parts of Andrographis paniculata	57:99	Diterpenoid Lactones with Anti-Inflammatory Effects from the Aerial Parts of Andrographis paniculata.
31357563	4	40	theme	diterpenoid	503:513	arg1	compounds					541:549	2 new compounds	535:549	2 new compounds	535:549	In this study, 17 diterpenoid lactones, including 2 new compounds, were identified by spectroscopic methods, and most of them attenuated the generation of TNF-α and IL-6 in LPS-induced RAW 274.7 cells examined by ELISA.
31357563	4	40	theme	diterpenoid	503:513	arg1	lactones					515:522	17 diterpenoid lactones	500:522	17 diterpenoid lactones	500:522	In this study, 17 diterpenoid lactones, including 2 new compounds, were identified by spectroscopic methods, and most of them attenuated the generation of TNF-α and IL-6 in LPS-induced RAW 274.7 cells examined by ELISA.
31357563	0	41	from	Parts	68:72	arg1	Effects					44:50	Anti-Inflammatory Effects	26:50	Anti-Inflammatory Effects from the Aerial Parts of Andrographis paniculata	26:99	Diterpenoid Lactones with Anti-Inflammatory Effects from the Aerial Parts of Andrographis paniculata.
31357563	7	42	theme	structure	1144:1152	arg1	modification					1154:1165	future structure modification	1137:1165	future structure modification	1137:1165	Our study provides guidelines for future structure modification and rational drug design of diterpenoid lactones with anti-inflammatory properties in medical chemistry.
31357563	7	43	theme	medical	1253:1259	arg1	chemistry					1261:1269	medical chemistry	1253:1269	medical chemistry	1253:1269	Our study provides guidelines for future structure modification and rational drug design of diterpenoid lactones with anti-inflammatory properties in medical chemistry.
31357563	7	44	from	properties	1239:1248	arg1	chemistry					1261:1269	medical chemistry	1253:1269	medical chemistry	1253:1269	Our study provides guidelines for future structure modification and rational drug design of diterpenoid lactones with anti-inflammatory properties in medical chemistry.
31357563	0	45	with	Lactones	12:19	arg1	Effects					44:50	Anti-Inflammatory Effects	26:50	Anti-Inflammatory Effects from the Aerial Parts of Andrographis paniculata	26:99	Diterpenoid Lactones with Anti-Inflammatory Effects from the Aerial Parts of Andrographis paniculata.
31357563	4	46	theme	274.7	674:678	arg1	cells					680:684	LPS-induced RAW 274.7 cells	658:684	LPS-induced RAW 274.7 cells examined by ELISA	658:702	In this study, 17 diterpenoid lactones, including 2 new compounds, were identified by spectroscopic methods, and most of them attenuated the generation of TNF-α and IL-6 in LPS-induced RAW 274.7 cells examined by ELISA.
31357563	6	47	from	activity	946:953	arg1	zebrafish					985:993	LPS microinjection-induced zebrafish	958:993	LPS microinjection-induced zebrafish	958:993	Moreover, new compounds (AP-1 and AP-4) exerted anti-inflammatory activity in LPS microinjection-induced zebrafish, which might be correlated with the inhibition of the translocation of NF-κB p65 from cytoplasm to nucleus.
31357563	7	48	with	design	1185:1190	arg1	properties					1239:1248	anti-inflammatory properties	1221:1248	anti-inflammatory properties in medical chemistry	1221:1269	Our study provides guidelines for future structure modification and rational drug design of diterpenoid lactones with anti-inflammatory properties in medical chemistry.
31357563	6	49	theme	NF-κB	1066:1070	arg1	p65					1072:1074	NF-κB p65	1066:1074	NF-κB p65	1066:1074	Moreover, new compounds (AP-1 and AP-4) exerted anti-inflammatory activity in LPS microinjection-induced zebrafish, which might be correlated with the inhibition of the translocation of NF-κB p65 from cytoplasm to nucleus.
31357563	3	50	theme	chemical	330:337	arg1	composition					339:349	systematic chemical composition	319:349	systematic chemical composition	319:349	However, systematic chemical composition and biological activities, as well as key pharmacophores, of these diterpenoid lactones from AP have not yet been clearly understood.
31357563	2	51	theme	Diterpenoid	240:250	arg1	components					292:301	the main anti-inflammatory components	265:301	the main anti-inflammatory components of AP	265:307	Diterpenoid lactones are the main anti-inflammatory components of AP.
31357563	2	51	theme	Diterpenoid	240:250	arg1	lactones					252:259	Diterpenoid lactones	240:259	Diterpenoid lactones	240:259	Diterpenoid lactones are the main anti-inflammatory components of AP.
31357563	5	52	theme	quantitative	820:831	arg1	relationship					852:863	quantitative structure-activity relationship	820:863	the quantitative structure-activity relationship (QSAR) models	816:877	Pharmacophores of diterpenoid lactones responsible for the anti-inflammatory activities were revealed based on the quantitative structure-activity relationship (QSAR) models.
31357563	5	52	theme	quantitative	820:831	arg1	QSAR					866:869	QSAR	866:869	QSAR	866:869	Pharmacophores of diterpenoid lactones responsible for the anti-inflammatory activities were revealed based on the quantitative structure-activity relationship (QSAR) models.
31357563	7	53	with	modification	1154:1165	arg1	properties					1239:1248	anti-inflammatory properties	1221:1248	anti-inflammatory properties in medical chemistry	1221:1269	Our study provides guidelines for future structure modification and rational drug design of diterpenoid lactones with anti-inflammatory properties in medical chemistry.
31357563	6	54	dep	compounds	894:902	arg1	AP-1					905:908	AP-1	905:908	AP-1	905:908	Moreover, new compounds (AP-1 and AP-4) exerted anti-inflammatory activity in LPS microinjection-induced zebrafish, which might be correlated with the inhibition of the translocation of NF-κB p65 from cytoplasm to nucleus.
31357563	6	54	dep	compounds	894:902	arg1	compounds					894:902	new compounds	890:902	new compounds (AP-1 and AP-4)	890:918	Moreover, new compounds (AP-1 and AP-4) exerted anti-inflammatory activity in LPS microinjection-induced zebrafish, which might be correlated with the inhibition of the translocation of NF-κB p65 from cytoplasm to nucleus.
31357563	6	54	dep	compounds	894:902	arg1	AP-4					914:917	AP-4	914:917	AP-4	914:917	Moreover, new compounds (AP-1 and AP-4) exerted anti-inflammatory activity in LPS microinjection-induced zebrafish, which might be correlated with the inhibition of the translocation of NF-κB p65 from cytoplasm to nucleus.
31357563	5	55	theme	lactones	735:742	arg1	Pharmacophores					705:718	Pharmacophores	705:718	Pharmacophores of diterpenoid lactones responsible for the anti-inflammatory activities	705:791	Pharmacophores of diterpenoid lactones responsible for the anti-inflammatory activities were revealed based on the quantitative structure-activity relationship (QSAR) models.
31357563	7	56	theme	drug	1180:1183	arg1	design					1185:1190	rational drug design	1171:1190	rational drug design	1171:1190	Our study provides guidelines for future structure modification and rational drug design of diterpenoid lactones with anti-inflammatory properties in medical chemistry.
31357563	4	57	theme	IL-6	650:653	arg1	generation					626:635	the generation	622:635	the generation of TNF-α and IL-6 in LPS-induced RAW 274.7 cells examined by ELISA	622:702	In this study, 17 diterpenoid lactones, including 2 new compounds, were identified by spectroscopic methods, and most of them attenuated the generation of TNF-α and IL-6 in LPS-induced RAW 274.7 cells examined by ELISA.
31357563	6	58	theme	anti-inflammatory	928:944	arg1	activity					946:953	anti-inflammatory activity	928:953	anti-inflammatory activity	928:953	Moreover, new compounds (AP-1 and AP-4) exerted anti-inflammatory activity in LPS microinjection-induced zebrafish, which might be correlated with the inhibition of the translocation of NF-κB p65 from cytoplasm to nucleus.
29664363	2	0	theme	spore-forming	110:122	arg1	bacterium					162:170	A Gram-variable, short-rod-shaped, motile, spore-forming, strictly aerobic and alkaliresistant bacterium	67:170	bacterium	162:170	A Gram-variable, short-rod-shaped, motile, spore-forming, strictly aerobic and alkaliresistant bacterium, designed strain J-3T, was isolated from farmland soil sampled in Yancheng city, Jiangsu province, China.
29664363	2	0	theme	spore-forming	110:122	arg1	J-3T					189:192	designed strain J-3T	173:192	designed strain J-3T	173:192	A Gram-variable, short-rod-shaped, motile, spore-forming, strictly aerobic and alkaliresistant bacterium, designed strain J-3T, was isolated from farmland soil sampled in Yancheng city, Jiangsu province, China.
29664363	3	1	dep	%	331:331	arg1	w/v					334:336	w/v	334:336	w/v	334:336	Optimal growth occurred at 30 °C, pH 7.0-8.0 and 0.5 % (w/v) NaCl.
29664363	4	2	theme	Paenibacillusripae	456:473	arg1	HZ1T					475:478	Paenibacillusripae HZ1T	456:478	Paenibacillusripae HZ1T (96.8 %)	456:487	Phylogenetic analysis based on the 16S rRNA gene sequences showed that strain J-3T was most closely related to Paenibacillusripae HZ1T (96.8 %), followed by Paenibacillussputi KIT00200-70066-1T (94.7 %).
29664363	4	2	theme	Paenibacillusripae	456:473	arg1	%					486:486	96.8 %	481:486	96.8 %	481:486	Phylogenetic analysis based on the 16S rRNA gene sequences showed that strain J-3T was most closely related to Paenibacillusripae HZ1T (96.8 %), followed by Paenibacillussputi KIT00200-70066-1T (94.7 %).
29664363	4	3	theme	Phylogenetic	345:356	arg1	analysis					358:365	Phylogenetic analysis	345:365	Phylogenetic analysis based on the 16S rRNA gene sequences	345:402	Phylogenetic analysis based on the 16S rRNA gene sequences showed that strain J-3T was most closely related to Paenibacillusripae HZ1T (96.8 %), followed by Paenibacillussputi KIT00200-70066-1T (94.7 %).
29664363	3	4	theme	0.5 	327:330	arg1	%					331:331	%	331:331	%	331:331	Optimal growth occurred at 30 °C, pH 7.0-8.0 and 0.5 % (w/v) NaCl.
29664363	2	5	theme	motile	102:107	arg1	bacterium					162:170	A Gram-variable, short-rod-shaped, motile, spore-forming, strictly aerobic and alkaliresistant bacterium	67:170	bacterium	162:170	A Gram-variable, short-rod-shaped, motile, spore-forming, strictly aerobic and alkaliresistant bacterium, designed strain J-3T, was isolated from farmland soil sampled in Yancheng city, Jiangsu province, China.
29664363	2	5	theme	motile	102:107	arg1	J-3T					189:192	designed strain J-3T	173:192	designed strain J-3T	173:192	A Gram-variable, short-rod-shaped, motile, spore-forming, strictly aerobic and alkaliresistant bacterium, designed strain J-3T, was isolated from farmland soil sampled in Yancheng city, Jiangsu province, China.
29664363	8	6	theme	meso-diaminopimelic	930:948	arg1	acid					890:893	The diagnostic diamino acid	867:893	The diagnostic diamino acid of the cell-wall peptidoglycan	867:924	The diagnostic diamino acid of the cell-wall peptidoglycan was meso-diaminopimelic acid.
29664363	8	6	theme	meso-diaminopimelic	930:948	arg1	acid					950:953	meso-diaminopimelic acid	930:953	meso-diaminopimelic acid	930:953	The diagnostic diamino acid of the cell-wall peptidoglycan was meso-diaminopimelic acid.
29664363	1	7	theme	farmland	52:59	arg1	soil					61:64	farmland soil	52:64	farmland soil	52:64	nov., isolated from farmland soil.
29664363	8	8	theme	peptidoglycan	912:924	arg1	acid					890:893	The diagnostic diamino acid	867:893	The diagnostic diamino acid of the cell-wall peptidoglycan	867:924	The diagnostic diamino acid of the cell-wall peptidoglycan was meso-diaminopimelic acid.
29664363	8	8	theme	peptidoglycan	912:924	arg1	acid					950:953	meso-diaminopimelic acid	930:953	meso-diaminopimelic acid	930:953	The diagnostic diamino acid of the cell-wall peptidoglycan was meso-diaminopimelic acid.
29664363	3	9	theme	%	331:331	arg1	NaCl					339:342	0.5 % (w/v) NaCl	327:342	0.5 % (w/v) NaCl	327:342	Optimal growth occurred at 30 °C, pH 7.0-8.0 and 0.5 % (w/v) NaCl.
29664363	2	10	theme	alkaliresistant	146:160	arg1	bacterium					162:170	A Gram-variable, short-rod-shaped, motile, spore-forming, strictly aerobic and alkaliresistant bacterium	67:170	bacterium	162:170	A Gram-variable, short-rod-shaped, motile, spore-forming, strictly aerobic and alkaliresistant bacterium, designed strain J-3T, was isolated from farmland soil sampled in Yancheng city, Jiangsu province, China.
29664363	2	10	theme	alkaliresistant	146:160	arg1	J-3T					189:192	designed strain J-3T	173:192	designed strain J-3T	173:192	A Gram-variable, short-rod-shaped, motile, spore-forming, strictly aerobic and alkaliresistant bacterium, designed strain J-3T, was isolated from farmland soil sampled in Yancheng city, Jiangsu province, China.
29664363	6	11	theme	respiratory	627:637	arg1	menaquinone-7					651:663	menaquinone-7	651:663	menaquinone-7	651:663	The dominant respiratory quinone was menaquinone-7 and the DNA G+C content was 41.2 mol%.
29664363	6	11	theme	respiratory	627:637	arg1	quinone					639:645	The dominant respiratory quinone	614:645	The dominant respiratory quinone	614:645	The dominant respiratory quinone was menaquinone-7 and the DNA G+C content was 41.2 mol%.
29664363	9	12	theme	novel	1028:1032	arg1	species					1034:1040	a novel species	1026:1040	a novel species	1026:1040	On the basis of genotypic and phenotypic data, strain J-3T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus yanchengensis sp.
29664363	4	13	theme	strain	416:421	arg1	J-3T					423:426	strain J-3T	416:426	strain J-3T	416:426	Phylogenetic analysis based on the 16S rRNA gene sequences showed that strain J-3T was most closely related to Paenibacillusripae HZ1T (96.8 %), followed by Paenibacillussputi KIT00200-70066-1T (94.7 %).
29664363	5	14	theme	major	553:557	arg1	anteiso-C15 					585:596	anteiso-C15 	585:596	anteiso-C15 	585:596	The major cellular fatty acids were anteiso-C15 : 0 and C16 : 0.
29664363	5	14	theme	major	553:557	arg1	acids					574:578	The major cellular fatty acids	549:578	The major cellular fatty acids	549:578	The major cellular fatty acids were anteiso-C15 : 0 and C16 : 0.
29664363	6	15	theme	dominant	618:625	arg1	menaquinone-7					651:663	menaquinone-7	651:663	menaquinone-7	651:663	The dominant respiratory quinone was menaquinone-7 and the DNA G+C content was 41.2 mol%.
29664363	6	15	theme	dominant	618:625	arg1	quinone					639:645	The dominant respiratory quinone	614:645	The dominant respiratory quinone	614:645	The dominant respiratory quinone was menaquinone-7 and the DNA G+C content was 41.2 mol%.
29664363	9	16	theme	genotypic	972:980	arg1	data					997:1000	genotypic and phenotypic data	972:1000	genotypic and phenotypic data	972:1000	On the basis of genotypic and phenotypic data, strain J-3T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus yanchengensis sp.
29664363	5	17	theme	cellular	559:566	arg1	anteiso-C15 					585:596	anteiso-C15 	585:596	anteiso-C15 	585:596	The major cellular fatty acids were anteiso-C15 : 0 and C16 : 0.
29664363	5	17	theme	cellular	559:566	arg1	acids					574:578	The major cellular fatty acids	549:578	The major cellular fatty acids	549:578	The major cellular fatty acids were anteiso-C15 : 0 and C16 : 0.
29664363	5	18	dep	 0	598:599	arg1	 0					610:611	 0	610:611	 0	610:611	The major cellular fatty acids were anteiso-C15 : 0 and C16 : 0.
29664363	2	19	attach	isolated	199:206	arg1	soil					222:225	farmland soil	213:225	farmland soil sampled in Yancheng city, Jiangsu province, China	213:275	A Gram-variable, short-rod-shaped, motile, spore-forming, strictly aerobic and alkaliresistant bacterium, designed strain J-3T, was isolated from farmland soil sampled in Yancheng city, Jiangsu province, China.
29664363	2	19	attach	isolated	199:206	arg2	bacterium					162:170	A Gram-variable, short-rod-shaped, motile, spore-forming, strictly aerobic and alkaliresistant bacterium	67:170	bacterium	162:170	A Gram-variable, short-rod-shaped, motile, spore-forming, strictly aerobic and alkaliresistant bacterium, designed strain J-3T, was isolated from farmland soil sampled in Yancheng city, Jiangsu province, China.
29664363	2	19	attach	isolated	199:206	arg2	J-3T					189:192	designed strain J-3T	173:192	designed strain J-3T	173:192	A Gram-variable, short-rod-shaped, motile, spore-forming, strictly aerobic and alkaliresistant bacterium, designed strain J-3T, was isolated from farmland soil sampled in Yancheng city, Jiangsu province, China.
29664363	0	20	theme	yanchengensis	14:26	arg1	sp					28:29	Paenibacillus yanchengensis sp	0:29	Paenibacillus yanchengensis sp.	0:30	Paenibacillus yanchengensis sp.
29664363	5	21	theme	fatty	568:572	arg1	anteiso-C15 					585:596	anteiso-C15 	585:596	anteiso-C15 	585:596	The major cellular fatty acids were anteiso-C15 : 0 and C16 : 0.
29664363	5	21	theme	fatty	568:572	arg1	acids					574:578	The major cellular fatty acids	549:578	The major cellular fatty acids	549:578	The major cellular fatty acids were anteiso-C15 : 0 and C16 : 0.
29664363	0	22	theme	Paenibacillus	0:12	arg1	sp					28:29	Paenibacillus yanchengensis sp	0:29	Paenibacillus yanchengensis sp.	0:30	Paenibacillus yanchengensis sp.
29664363	9	23	theme	genus	1049:1053	arg1	species					1034:1040	a novel species	1026:1040	a novel species	1026:1040	On the basis of genotypic and phenotypic data, strain J-3T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus yanchengensis sp.
29664363	4	24	theme	gene	389:392	arg1	sequences					394:402	the 16S rRNA gene sequences	376:402	the 16S rRNA gene sequences	376:402	Phylogenetic analysis based on the 16S rRNA gene sequences showed that strain J-3T was most closely related to Paenibacillusripae HZ1T (96.8 %), followed by Paenibacillussputi KIT00200-70066-1T (94.7 %).
29664363	9	25	theme	yanchengensis	1103:1115	arg1	sp					1117:1118	the name Paenibacillus yanchengensis sp	1080:1118	the name Paenibacillus yanchengensis sp	1080:1118	On the basis of genotypic and phenotypic data, strain J-3T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus yanchengensis sp.
29664363	11	26	theme	33926T=CGMCC	1170:1181	arg1	1.16455T					1183:1190	=KCTC 33926T=CGMCC 1.16455T	1164:1190	=KCTC 33926T=CGMCC 1.16455T	1164:1190	The type strain is J-3T (=KCTC 33926T=CGMCC 1.16455T).
29664363	11	26	theme	33926T=CGMCC	1170:1181	arg1	J-3T					1158:1161	J-3T	1158:1161	J-3T (=KCTC 33926T=CGMCC 1.16455T)	1158:1191	The type strain is J-3T (=KCTC 33926T=CGMCC 1.16455T).
29664363	6	27	theme	41.2 mol	693:700	arg1	G+C content					677:687	the DNA G+C content	669:687	the DNA G+C content	669:687	The dominant respiratory quinone was menaquinone-7 and the DNA G+C content was 41.2 mol%.
29664363	6	27	theme	41.2 mol	693:700	arg1	%					701:701	41.2 mol%	693:701	41.2 mol%	693:701	The dominant respiratory quinone was menaquinone-7 and the DNA G+C content was 41.2 mol%.
29664363	11	28	theme	type	1143:1146	arg1	strain					1148:1153	The type strain	1139:1153	The type strain	1139:1153	The type strain is J-3T (=KCTC 33926T=CGMCC 1.16455T).
29664363	11	28	theme	type	1143:1146	arg1	J-3T					1158:1161	J-3T	1158:1161	J-3T (=KCTC 33926T=CGMCC 1.16455T)	1158:1191	The type strain is J-3T (=KCTC 33926T=CGMCC 1.16455T).
29664363	9	29	theme	Paenibacillus	1055:1067	arg1	genus					1049:1053	the genus Paenibacillus	1045:1067	the genus Paenibacillus	1045:1067	On the basis of genotypic and phenotypic data, strain J-3T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus yanchengensis sp.
29664363	5	30	dep	anteiso-C15 	585:596	arg1	C16 					605:608	C16 	605:608	C16 	605:608	The major cellular fatty acids were anteiso-C15 : 0 and C16 : 0.
29664363	5	30	dep	anteiso-C15 	585:596	arg1	 0					598:599	 0	598:599	 0	598:599	The major cellular fatty acids were anteiso-C15 : 0 and C16 : 0.
29664363	7	31	theme	major	708:712	arg1	aminolipid					747:756	aminolipid	747:756	aminolipid	747:756	The major polar lipids of strain J-3T were aminolipid, phospholipid, diphosphatidylglycerol, phosphatidylglycerol, phosphoaminolipid and phosphatidylethanolamine.
29664363	7	31	theme	major	708:712	arg1	lipids					720:725	The major polar lipids	704:725	The major polar lipids of strain J-3T	704:740	The major polar lipids of strain J-3T were aminolipid, phospholipid, diphosphatidylglycerol, phosphatidylglycerol, phosphoaminolipid and phosphatidylethanolamine.
29664363	9	32	theme	name	1084:1087	arg1	sp					1117:1118	the name Paenibacillus yanchengensis sp	1080:1118	the name Paenibacillus yanchengensis sp	1080:1118	On the basis of genotypic and phenotypic data, strain J-3T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus yanchengensis sp.
29664363	2	33	theme	strain	182:187	arg1	bacterium					162:170	A Gram-variable, short-rod-shaped, motile, spore-forming, strictly aerobic and alkaliresistant bacterium	67:170	bacterium	162:170	A Gram-variable, short-rod-shaped, motile, spore-forming, strictly aerobic and alkaliresistant bacterium, designed strain J-3T, was isolated from farmland soil sampled in Yancheng city, Jiangsu province, China.
29664363	2	33	theme	strain	182:187	arg1	J-3T					189:192	designed strain J-3T	173:192	designed strain J-3T	173:192	A Gram-variable, short-rod-shaped, motile, spore-forming, strictly aerobic and alkaliresistant bacterium, designed strain J-3T, was isolated from farmland soil sampled in Yancheng city, Jiangsu province, China.
29664363	7	34	theme	strain	730:735	arg1	J-3T					737:740	strain J-3T	730:740	strain J-3T	730:740	The major polar lipids of strain J-3T were aminolipid, phospholipid, diphosphatidylglycerol, phosphatidylglycerol, phosphoaminolipid and phosphatidylethanolamine.
29664363	7	35	theme	polar	714:718	arg1	aminolipid					747:756	aminolipid	747:756	aminolipid	747:756	The major polar lipids of strain J-3T were aminolipid, phospholipid, diphosphatidylglycerol, phosphatidylglycerol, phosphoaminolipid and phosphatidylethanolamine.
29664363	7	35	theme	polar	714:718	arg1	lipids					720:725	The major polar lipids	704:725	The major polar lipids of strain J-3T	704:740	The major polar lipids of strain J-3T were aminolipid, phospholipid, diphosphatidylglycerol, phosphatidylglycerol, phosphoaminolipid and phosphatidylethanolamine.
29664363	2	36	theme	designed	173:180	arg1	bacterium					162:170	A Gram-variable, short-rod-shaped, motile, spore-forming, strictly aerobic and alkaliresistant bacterium	67:170	bacterium	162:170	A Gram-variable, short-rod-shaped, motile, spore-forming, strictly aerobic and alkaliresistant bacterium, designed strain J-3T, was isolated from farmland soil sampled in Yancheng city, Jiangsu province, China.
29664363	2	36	theme	designed	173:180	arg1	J-3T					189:192	designed strain J-3T	173:192	designed strain J-3T	173:192	A Gram-variable, short-rod-shaped, motile, spore-forming, strictly aerobic and alkaliresistant bacterium, designed strain J-3T, was isolated from farmland soil sampled in Yancheng city, Jiangsu province, China.
29664363	7	37	theme	J-3T	737:740	arg1	aminolipid					747:756	aminolipid	747:756	aminolipid	747:756	The major polar lipids of strain J-3T were aminolipid, phospholipid, diphosphatidylglycerol, phosphatidylglycerol, phosphoaminolipid and phosphatidylethanolamine.
29664363	7	37	theme	J-3T	737:740	arg1	lipids					720:725	The major polar lipids	704:725	The major polar lipids of strain J-3T	704:740	The major polar lipids of strain J-3T were aminolipid, phospholipid, diphosphatidylglycerol, phosphatidylglycerol, phosphoaminolipid and phosphatidylethanolamine.
29664363	11	38	theme	=KCTC	1164:1168	arg1	1.16455T					1183:1190	=KCTC 33926T=CGMCC 1.16455T	1164:1190	=KCTC 33926T=CGMCC 1.16455T	1164:1190	The type strain is J-3T (=KCTC 33926T=CGMCC 1.16455T).
29664363	11	38	theme	=KCTC	1164:1168	arg1	J-3T					1158:1161	J-3T	1158:1161	J-3T (=KCTC 33926T=CGMCC 1.16455T)	1158:1191	The type strain is J-3T (=KCTC 33926T=CGMCC 1.16455T).
29664363	8	39	theme	diamino	882:888	arg1	acid					890:893	The diagnostic diamino acid	867:893	The diagnostic diamino acid of the cell-wall peptidoglycan	867:924	The diagnostic diamino acid of the cell-wall peptidoglycan was meso-diaminopimelic acid.
29664363	8	39	theme	diamino	882:888	arg1	acid					950:953	meso-diaminopimelic acid	930:953	meso-diaminopimelic acid	930:953	The diagnostic diamino acid of the cell-wall peptidoglycan was meso-diaminopimelic acid.
29664363	2	40	theme	short-rod-shaped	84:99	arg1	bacterium					162:170	A Gram-variable, short-rod-shaped, motile, spore-forming, strictly aerobic and alkaliresistant bacterium	67:170	bacterium	162:170	A Gram-variable, short-rod-shaped, motile, spore-forming, strictly aerobic and alkaliresistant bacterium, designed strain J-3T, was isolated from farmland soil sampled in Yancheng city, Jiangsu province, China.
29664363	2	40	theme	short-rod-shaped	84:99	arg1	J-3T					189:192	designed strain J-3T	173:192	designed strain J-3T	173:192	A Gram-variable, short-rod-shaped, motile, spore-forming, strictly aerobic and alkaliresistant bacterium, designed strain J-3T, was isolated from farmland soil sampled in Yancheng city, Jiangsu province, China.
29664363	2	41	theme	farmland	213:220	arg1	soil					222:225	farmland soil	213:225	farmland soil sampled in Yancheng city, Jiangsu province, China	213:275	A Gram-variable, short-rod-shaped, motile, spore-forming, strictly aerobic and alkaliresistant bacterium, designed strain J-3T, was isolated from farmland soil sampled in Yancheng city, Jiangsu province, China.
29664363	8	42	theme	diagnostic	871:880	arg1	acid					890:893	The diagnostic diamino acid	867:893	The diagnostic diamino acid of the cell-wall peptidoglycan	867:924	The diagnostic diamino acid of the cell-wall peptidoglycan was meso-diaminopimelic acid.
29664363	8	42	theme	diagnostic	871:880	arg1	acid					950:953	meso-diaminopimelic acid	930:953	meso-diaminopimelic acid	930:953	The diagnostic diamino acid of the cell-wall peptidoglycan was meso-diaminopimelic acid.
29664363	2	43	dep	short-rod-shaped	84:99	arg1	aerobic					134:140	aerobic	134:140	aerobic	134:140	A Gram-variable, short-rod-shaped, motile, spore-forming, strictly aerobic and alkaliresistant bacterium, designed strain J-3T, was isolated from farmland soil sampled in Yancheng city, Jiangsu province, China.
29664363	4	44	dep	related	445:451	arg1	followed					490:497	followed	490:497	followed by Paenibacillussputi KIT00200-70066-1T (94.7 %)	490:546	Phylogenetic analysis based on the 16S rRNA gene sequences showed that strain J-3T was most closely related to Paenibacillusripae HZ1T (96.8 %), followed by Paenibacillussputi KIT00200-70066-1T (94.7 %).
29664363	6	45	theme	DNA	673:675	arg1	G+C content					677:687	the DNA G+C content	669:687	the DNA G+C content	669:687	The dominant respiratory quinone was menaquinone-7 and the DNA G+C content was 41.2 mol%.
29664363	6	45	theme	DNA	673:675	arg1	%					701:701	41.2 mol%	693:701	41.2 mol%	693:701	The dominant respiratory quinone was menaquinone-7 and the DNA G+C content was 41.2 mol%.
29664363	2	46	theme	Gram-variable	69:81	arg1	bacterium					162:170	A Gram-variable, short-rod-shaped, motile, spore-forming, strictly aerobic and alkaliresistant bacterium	67:170	bacterium	162:170	A Gram-variable, short-rod-shaped, motile, spore-forming, strictly aerobic and alkaliresistant bacterium, designed strain J-3T, was isolated from farmland soil sampled in Yancheng city, Jiangsu province, China.
29664363	2	46	theme	Gram-variable	69:81	arg1	J-3T					189:192	designed strain J-3T	173:192	designed strain J-3T	173:192	A Gram-variable, short-rod-shaped, motile, spore-forming, strictly aerobic and alkaliresistant bacterium, designed strain J-3T, was isolated from farmland soil sampled in Yancheng city, Jiangsu province, China.
29664363	9	47	theme	Paenibacillus	1089:1101	arg1	sp					1117:1118	the name Paenibacillus yanchengensis sp	1080:1118	the name Paenibacillus yanchengensis sp	1080:1118	On the basis of genotypic and phenotypic data, strain J-3T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus yanchengensis sp.
29664363	9	48	theme	phenotypic	986:995	arg1	data					997:1000	genotypic and phenotypic data	972:1000	genotypic and phenotypic data	972:1000	On the basis of genotypic and phenotypic data, strain J-3T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus yanchengensis sp.
29664363	3	49	theme	Optimal	278:284	arg1	growth					286:291	Optimal growth	278:291	Optimal growth	278:291	Optimal growth occurred at 30 °C, pH 7.0-8.0 and 0.5 % (w/v) NaCl.
29664363	4	50	theme	rRNA	384:387	arg1	sequences					394:402	the 16S rRNA gene sequences	376:402	the 16S rRNA gene sequences	376:402	Phylogenetic analysis based on the 16S rRNA gene sequences showed that strain J-3T was most closely related to Paenibacillusripae HZ1T (96.8 %), followed by Paenibacillussputi KIT00200-70066-1T (94.7 %).
29664363	9	51	dep	data	997:1000	arg1	basis					963:967	basis	963:967	basis	963:967	On the basis of genotypic and phenotypic data, strain J-3T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus yanchengensis sp.
29664363	9	51	dep	data	997:1000	arg1	the					959:961	the	959:961	the	959:961	On the basis of genotypic and phenotypic data, strain J-3T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus yanchengensis sp.
29664363	8	52	theme	cell-wall	902:910	arg1	peptidoglycan					912:924	the cell-wall peptidoglycan	898:924	the cell-wall peptidoglycan	898:924	The diagnostic diamino acid of the cell-wall peptidoglycan was meso-diaminopimelic acid.
29664363	4	53	theme	16S	380:382	arg1	sequences					394:402	the 16S rRNA gene sequences	376:402	the 16S rRNA gene sequences	376:402	Phylogenetic analysis based on the 16S rRNA gene sequences showed that strain J-3T was most closely related to Paenibacillusripae HZ1T (96.8 %), followed by Paenibacillussputi KIT00200-70066-1T (94.7 %).
29664363	3	54	theme	pH	312:313	arg1	7.0-8.0					315:321	pH 7.0-8.0	312:321	pH 7.0-8.0	312:321	Optimal growth occurred at 30 °C, pH 7.0-8.0 and 0.5 % (w/v) NaCl.
29664363	9	55	theme	strain	1003:1008	arg1	J-3T					1010:1013	strain J-3T	1003:1013	strain J-3T	1003:1013	On the basis of genotypic and phenotypic data, strain J-3T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus yanchengensis sp.
29458566	12	0	theme	name	1388:1391	arg1	sp					1418:1419	the name Paenibacillustranslucens sp	1384:1419	the name Paenibacillustranslucens sp	1384:1419	On the basis of the polyphasic taxonomic study, strain CJ11T represents a novel species in the genus Paenibacillus, for which the name Paenibacillustranslucens sp.
29458566	11	1	theme	unidentified	1237:1248	arg1	lipids					1250:1255	several unidentified lipids	1229:1255	several unidentified lipids	1229:1255	The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two unidentified phospholipids, an unidentified glycolipid and several unidentified lipids.
29458566	8	2	theme	meso-diaminopimelic	877:895	arg1	acid					897:900	meso-diaminopimelic acid	877:900	meso-diaminopimelic acid in the cell-wall peptidoglycan	877:931	Strain CJ11Tcontained meso-diaminopimelic acid in the cell-wall peptidoglycan.
29458566	6	3	theme	DNA-DNA	705:711	arg1	levels					725:730	DNA-DNA relatedness levels	705:730	DNA-DNA relatedness levels of strain CJ11T	705:746	DNA-DNA relatedness levels of strain CJ11T was 41.7 % (reciprocal, 57.8 %) to P. lacus KCTC 33691T.
29458566	2	4	theme	rod-shaped	103:112	arg1	strain					150:155	A Gram-stain-variable, aerobic, rod-shaped, motile and spore-forming bacterial strain	71:155	A Gram-stain-variable, aerobic, rod-shaped, motile and spore-forming bacterial strain	71:155	A Gram-stain-variable, aerobic, rod-shaped, motile and spore-forming bacterial strain, designated CJ11T, was isolated from a tidal flat sediment sample from Ganghwa-do, Republic of Korea.
29458566	14	5	theme	=KACC	1466:1470	arg1	CJ11T					1459:1463	CJ11T	1459:1463	CJ11T (=KACC 19304T=JCM 32080T)	1459:1489	The type strain is CJ11T (=KACC 19304T=JCM 32080T).
29458566	14	5	theme	=KACC	1466:1470	arg1	32080T					1483:1488	=KACC 19304T=JCM 32080T	1466:1488	=KACC 19304T=JCM 32080T	1466:1488	The type strain is CJ11T (=KACC 19304T=JCM 32080T).
29458566	4	6	theme	gene	350:353	arg1	results					326:332	Sequencing results	315:332	Sequencing results of the 16S rRNA gene	315:353	Sequencing results of the 16S rRNA gene revealed that strain CJ11T possesses two copies of the 16S rRNA gene varying at five nucleotide positions.
29458566	11	7	theme	several	1229:1235	arg1	lipids					1250:1255	several unidentified lipids	1229:1255	several unidentified lipids	1229:1255	The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two unidentified phospholipids, an unidentified glycolipid and several unidentified lipids.
29458566	5	8	theme	99.36-99.15 	678:689	arg1	 similarity					691:701	99.36-99.15 % similarity	678:701	99.36-99.15 % similarity	678:701	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CJ11T belonged to the genus Paenibacillus within the family Paenibacillaceae and was most closely related to Paenibacillus lacus KCTC 33691T (99.36-99.15 % similarity).
29458566	5	8	theme	99.36-99.15 	678:689	arg1	33691T					670:675	Paenibacillus lacus KCTC 33691T	645:675	Paenibacillus lacus KCTC 33691T (99.36-99.15 % similarity)	645:702	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CJ11T belonged to the genus Paenibacillus within the family Paenibacillaceae and was most closely related to Paenibacillus lacus KCTC 33691T (99.36-99.15 % similarity).
29458566	11	9	theme	predominant	1064:1074	arg1	diphosphatidylglycerol					1094:1115	diphosphatidylglycerol	1094:1115	diphosphatidylglycerol	1094:1115	The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two unidentified phospholipids, an unidentified glycolipid and several unidentified lipids.
29458566	11	9	theme	predominant	1064:1074	arg1	lipids					1082:1087	The predominant polar lipids	1060:1087	The predominant polar lipids	1060:1087	The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two unidentified phospholipids, an unidentified glycolipid and several unidentified lipids.
29458566	4	10	theme	rRNA	345:348	arg1	gene					350:353	the 16S rRNA gene	337:353	the 16S rRNA gene	337:353	Sequencing results of the 16S rRNA gene revealed that strain CJ11T possesses two copies of the 16S rRNA gene varying at five nucleotide positions.
29458566	1	11	theme	tidal	50:54	arg1	sediment					61:68	tidal flat sediment	50:68	tidal flat sediment	50:68	nov., isolated from tidal flat sediment.
29458566	5	12	theme	%	690:690	arg1	 similarity					691:701	99.36-99.15 % similarity	678:701	99.36-99.15 % similarity	678:701	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CJ11T belonged to the genus Paenibacillus within the family Paenibacillaceae and was most closely related to Paenibacillus lacus KCTC 33691T (99.36-99.15 % similarity).
29458566	5	12	theme	%	690:690	arg1	33691T					670:675	Paenibacillus lacus KCTC 33691T	645:675	Paenibacillus lacus KCTC 33691T (99.36-99.15 % similarity)	645:702	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CJ11T belonged to the genus Paenibacillus within the family Paenibacillaceae and was most closely related to Paenibacillus lacus KCTC 33691T (99.36-99.15 % similarity).
29458566	4	13	theme	gene	419:422	arg1	copies					396:401	two copies	392:401	two copies of the 16S rRNA gene varying at five nucleotide positions	392:459	Sequencing results of the 16S rRNA gene revealed that strain CJ11T possesses two copies of the 16S rRNA gene varying at five nucleotide positions.
29458566	6	14	theme	relatedness	713:723	arg1	levels					725:730	DNA-DNA relatedness levels	705:730	DNA-DNA relatedness levels of strain CJ11T	705:746	DNA-DNA relatedness levels of strain CJ11T was 41.7 % (reciprocal, 57.8 %) to P. lacus KCTC 33691T.
29458566	1	15	theme	flat	56:59	arg1	sediment					61:68	tidal flat sediment	50:68	tidal flat sediment	50:68	nov., isolated from tidal flat sediment.
29458566	4	16	theme	rRNA	414:417	arg1	gene					419:422	the 16S rRNA gene	406:422	the 16S rRNA gene varying at five nucleotide positions	406:459	Sequencing results of the 16S rRNA gene revealed that strain CJ11T possesses two copies of the 16S rRNA gene varying at five nucleotide positions.
29458566	5	17	theme	Paenibacillus	564:576	arg1	genus					558:562	the genus Paenibacillus	554:576	the genus Paenibacillus within the family Paenibacillaceae	554:611	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CJ11T belonged to the genus Paenibacillus within the family Paenibacillaceae and was most closely related to Paenibacillus lacus KCTC 33691T (99.36-99.15 % similarity).
29458566	8	18	theme	cell-wall	909:917	arg1	peptidoglycan					919:931	the cell-wall peptidoglycan	905:931	the cell-wall peptidoglycan	905:931	Strain CJ11Tcontained meso-diaminopimelic acid in the cell-wall peptidoglycan.
29458566	11	19	theme	polar	1076:1080	arg1	diphosphatidylglycerol					1094:1115	diphosphatidylglycerol	1094:1115	diphosphatidylglycerol	1094:1115	The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two unidentified phospholipids, an unidentified glycolipid and several unidentified lipids.
29458566	11	19	theme	polar	1076:1080	arg1	lipids					1082:1087	The predominant polar lipids	1060:1087	The predominant polar lipids	1060:1087	The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two unidentified phospholipids, an unidentified glycolipid and several unidentified lipids.
29458566	4	20	contain	possesses	382:390	arg1	CJ11T					376:380	strain CJ11T	369:380	strain CJ11T	369:380	Sequencing results of the 16S rRNA gene revealed that strain CJ11T possesses two copies of the 16S rRNA gene varying at five nucleotide positions.
29458566	4	20	contain	possesses	382:390	arg2	copies					396:401	two copies	392:401	two copies of the 16S rRNA gene varying at five nucleotide positions	392:459	Sequencing results of the 16S rRNA gene revealed that strain CJ11T possesses two copies of the 16S rRNA gene varying at five nucleotide positions.
29458566	2	21	theme	spore-forming	126:138	arg1	strain					150:155	A Gram-stain-variable, aerobic, rod-shaped, motile and spore-forming bacterial strain	71:155	A Gram-stain-variable, aerobic, rod-shaped, motile and spore-forming bacterial strain	71:155	A Gram-stain-variable, aerobic, rod-shaped, motile and spore-forming bacterial strain, designated CJ11T, was isolated from a tidal flat sediment sample from Ganghwa-do, Republic of Korea.
29458566	14	22	theme	19304T=JCM	1472:1481	arg1	CJ11T					1459:1463	CJ11T	1459:1463	CJ11T (=KACC 19304T=JCM 32080T)	1459:1489	The type strain is CJ11T (=KACC 19304T=JCM 32080T).
29458566	14	22	theme	19304T=JCM	1472:1481	arg1	32080T					1483:1488	=KACC 19304T=JCM 32080T	1466:1488	=KACC 19304T=JCM 32080T	1466:1488	The type strain is CJ11T (=KACC 19304T=JCM 32080T).
29458566	10	23	theme	cellular	992:999	arg1	anteiso-C15 					1018:1029	anteiso-C15 	1018:1029	anteiso-C15 	1018:1029	The major cellular fatty acids were anteiso-C15 : 0, C16 : 0 and iso-C16 : 0.
29458566	10	23	theme	cellular	992:999	arg1	acids					1007:1011	The major cellular fatty acids	982:1011	The major cellular fatty acids	982:1011	The major cellular fatty acids were anteiso-C15 : 0, C16 : 0 and iso-C16 : 0.
29458566	6	24	theme	CJ11T	742:746	arg1	levels					725:730	DNA-DNA relatedness levels	705:730	DNA-DNA relatedness levels of strain CJ11T	705:746	DNA-DNA relatedness levels of strain CJ11T was 41.7 % (reciprocal, 57.8 %) to P. lacus KCTC 33691T.
29458566	2	25	theme	bacterial	140:148	arg1	strain					150:155	A Gram-stain-variable, aerobic, rod-shaped, motile and spore-forming bacterial strain	71:155	A Gram-stain-variable, aerobic, rod-shaped, motile and spore-forming bacterial strain	71:155	A Gram-stain-variable, aerobic, rod-shaped, motile and spore-forming bacterial strain, designated CJ11T, was isolated from a tidal flat sediment sample from Ganghwa-do, Republic of Korea.
29458566	0	26	theme	Paenibacillus	0:12	arg1	translucens					14:24	Paenibacillus translucens	0:24	Paenibacillus translucens	0:24	Paenibacillus translucens sp.
29458566	5	27	theme	family	589:594	arg1	Paenibacillaceae					596:611	the family Paenibacillaceae	585:611	the family Paenibacillaceae	585:611	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CJ11T belonged to the genus Paenibacillus within the family Paenibacillaceae and was most closely related to Paenibacillus lacus KCTC 33691T (99.36-99.15 % similarity).
29458566	4	28	theme	16S	341:343	arg1	gene					350:353	the 16S rRNA gene	337:353	the 16S rRNA gene	337:353	Sequencing results of the 16S rRNA gene revealed that strain CJ11T possesses two copies of the 16S rRNA gene varying at five nucleotide positions.
29458566	12	29	from	species	1338:1344	arg1	genus					1353:1357	the genus Paenibacillus	1349:1371	the genus Paenibacillus	1349:1371	On the basis of the polyphasic taxonomic study, strain CJ11T represents a novel species in the genus Paenibacillus, for which the name Paenibacillustranslucens sp.
29458566	2	30	attach	isolated	180:187	arg2	strain					150:155	A Gram-stain-variable, aerobic, rod-shaped, motile and spore-forming bacterial strain	71:155	A Gram-stain-variable, aerobic, rod-shaped, motile and spore-forming bacterial strain	71:155	A Gram-stain-variable, aerobic, rod-shaped, motile and spore-forming bacterial strain, designated CJ11T, was isolated from a tidal flat sediment sample from Ganghwa-do, Republic of Korea.
29458566	2	30	attach	isolated	180:187	arg1	sample					216:221	a tidal flat sediment sample	194:221	a tidal flat sediment sample from Ganghwa-do, Republic of Korea	194:256	A Gram-stain-variable, aerobic, rod-shaped, motile and spore-forming bacterial strain, designated CJ11T, was isolated from a tidal flat sediment sample from Ganghwa-do, Republic of Korea.
29458566	5	31	theme	strain	529:534	arg1	CJ11T					536:540	strain CJ11T	529:540	strain CJ11T	529:540	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CJ11T belonged to the genus Paenibacillus within the family Paenibacillaceae and was most closely related to Paenibacillus lacus KCTC 33691T (99.36-99.15 % similarity).
29458566	5	32	theme	lacus	659:663	arg1	 similarity					691:701	99.36-99.15 % similarity	678:701	99.36-99.15 % similarity	678:701	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CJ11T belonged to the genus Paenibacillus within the family Paenibacillaceae and was most closely related to Paenibacillus lacus KCTC 33691T (99.36-99.15 % similarity).
29458566	5	32	theme	lacus	659:663	arg1	33691T					670:675	Paenibacillus lacus KCTC 33691T	645:675	Paenibacillus lacus KCTC 33691T (99.36-99.15 % similarity)	645:702	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CJ11T belonged to the genus Paenibacillus within the family Paenibacillaceae and was most closely related to Paenibacillus lacus KCTC 33691T (99.36-99.15 % similarity).
29458566	7	33	theme	51.0 mol	844:851	arg1	%					852:852	51.0 mol%	844:852	51.0 mol%	844:852	The G+C content of the genomic DNA was 51.0 mol%.
29458566	7	33	theme	51.0 mol	844:851	arg1	G+C content					809:819	The G+C content	805:819	The G+C content of the genomic DNA	805:838	The G+C content of the genomic DNA was 51.0 mol%.
29458566	8	34	from	acid	897:900	arg1	peptidoglycan					919:931	the cell-wall peptidoglycan	905:931	the cell-wall peptidoglycan	905:931	Strain CJ11Tcontained meso-diaminopimelic acid in the cell-wall peptidoglycan.
29458566	6	35	theme	strain	735:740	arg1	CJ11T					742:746	strain CJ11T	735:746	strain CJ11T	735:746	DNA-DNA relatedness levels of strain CJ11T was 41.7 % (reciprocal, 57.8 %) to P. lacus KCTC 33691T.
29458566	5	36	theme	KCTC	665:668	arg1	 similarity					691:701	99.36-99.15 % similarity	678:701	99.36-99.15 % similarity	678:701	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CJ11T belonged to the genus Paenibacillus within the family Paenibacillaceae and was most closely related to Paenibacillus lacus KCTC 33691T (99.36-99.15 % similarity).
29458566	5	36	theme	KCTC	665:668	arg1	33691T					670:675	Paenibacillus lacus KCTC 33691T	645:675	Paenibacillus lacus KCTC 33691T (99.36-99.15 % similarity)	645:702	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CJ11T belonged to the genus Paenibacillus within the family Paenibacillaceae and was most closely related to Paenibacillus lacus KCTC 33691T (99.36-99.15 % similarity).
29458566	12	37	theme	novel	1332:1336	arg1	species					1338:1344	a novel species	1330:1344	a novel species	1330:1344	On the basis of the polyphasic taxonomic study, strain CJ11T represents a novel species in the genus Paenibacillus, for which the name Paenibacillustranslucens sp.
29458566	2	38	from	Republic	240:247	arg1	sample					216:221	a tidal flat sediment sample	194:221	a tidal flat sediment sample from Ganghwa-do, Republic of Korea	194:256	A Gram-stain-variable, aerobic, rod-shaped, motile and spore-forming bacterial strain, designated CJ11T, was isolated from a tidal flat sediment sample from Ganghwa-do, Republic of Korea.
29458566	11	39	theme	unidentified	1170:1181	arg1	phospholipids					1183:1195	two unidentified phospholipids	1166:1195	two unidentified phospholipids	1166:1195	The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two unidentified phospholipids, an unidentified glycolipid and several unidentified lipids.
29458566	6	40	dep	reciprocal	760:769	arg1	%					777:777	57.8 %	772:777	57.8 %	772:777	DNA-DNA relatedness levels of strain CJ11T was 41.7 % (reciprocal, 57.8 %) to P. lacus KCTC 33691T.
29458566	5	41	theme	gene	502:505	arg1	sequences					507:515	16S rRNA gene sequences	493:515	16S rRNA gene sequences	493:515	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CJ11T belonged to the genus Paenibacillus within the family Paenibacillaceae and was most closely related to Paenibacillus lacus KCTC 33691T (99.36-99.15 % similarity).
29458566	2	42	theme	aerobic	94:100	arg1	strain					150:155	A Gram-stain-variable, aerobic, rod-shaped, motile and spore-forming bacterial strain	71:155	A Gram-stain-variable, aerobic, rod-shaped, motile and spore-forming bacterial strain	71:155	A Gram-stain-variable, aerobic, rod-shaped, motile and spore-forming bacterial strain, designated CJ11T, was isolated from a tidal flat sediment sample from Ganghwa-do, Republic of Korea.
29458566	4	43	theme	16S	410:412	arg1	gene					419:422	the 16S rRNA gene	406:422	the 16S rRNA gene varying at five nucleotide positions	406:459	Sequencing results of the 16S rRNA gene revealed that strain CJ11T possesses two copies of the 16S rRNA gene varying at five nucleotide positions.
29458566	2	44	theme	sediment	207:214	arg1	sample					216:221	a tidal flat sediment sample	194:221	a tidal flat sediment sample from Ganghwa-do, Republic of Korea	194:256	A Gram-stain-variable, aerobic, rod-shaped, motile and spore-forming bacterial strain, designated CJ11T, was isolated from a tidal flat sediment sample from Ganghwa-do, Republic of Korea.
29458566	5	45	theme	16S	493:495	arg1	sequences					507:515	16S rRNA gene sequences	493:515	16S rRNA gene sequences	493:515	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CJ11T belonged to the genus Paenibacillus within the family Paenibacillaceae and was most closely related to Paenibacillus lacus KCTC 33691T (99.36-99.15 % similarity).
29458566	12	46	dep	study	1299:1303	arg1	the					1261:1263	the	1261:1263	the	1261:1263	On the basis of the polyphasic taxonomic study, strain CJ11T represents a novel species in the genus Paenibacillus, for which the name Paenibacillustranslucens sp.
29458566	12	46	dep	study	1299:1303	arg1	basis					1265:1269	basis	1265:1269	basis	1265:1269	On the basis of the polyphasic taxonomic study, strain CJ11T represents a novel species in the genus Paenibacillus, for which the name Paenibacillustranslucens sp.
29458566	9	47	theme	major	938:942	arg1	menaquinone-7					967:979	menaquinone-7	967:979	menaquinone-7	967:979	The major isoprenoid quinone was menaquinone-7.
29458566	9	47	theme	major	938:942	arg1	quinone					955:961	The major isoprenoid quinone	934:961	The major isoprenoid quinone	934:961	The major isoprenoid quinone was menaquinone-7.
29458566	11	48	theme	unidentified	1201:1212	arg1	glycolipid					1214:1223	an unidentified glycolipid	1198:1223	an unidentified glycolipid	1198:1223	The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two unidentified phospholipids, an unidentified glycolipid and several unidentified lipids.
29458566	2	49	theme	flat	202:205	arg1	sample					216:221	a tidal flat sediment sample	194:221	a tidal flat sediment sample from Ganghwa-do, Republic of Korea	194:256	A Gram-stain-variable, aerobic, rod-shaped, motile and spore-forming bacterial strain, designated CJ11T, was isolated from a tidal flat sediment sample from Ganghwa-do, Republic of Korea.
29458566	5	50	theme	rRNA	497:500	arg1	sequences					507:515	16S rRNA gene sequences	493:515	16S rRNA gene sequences	493:515	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CJ11T belonged to the genus Paenibacillus within the family Paenibacillaceae and was most closely related to Paenibacillus lacus KCTC 33691T (99.36-99.15 % similarity).
29458566	9	51	theme	isoprenoid	944:953	arg1	menaquinone-7					967:979	menaquinone-7	967:979	menaquinone-7	967:979	The major isoprenoid quinone was menaquinone-7.
29458566	9	51	theme	isoprenoid	944:953	arg1	quinone					955:961	The major isoprenoid quinone	934:961	The major isoprenoid quinone	934:961	The major isoprenoid quinone was menaquinone-7.
29458566	2	52	theme	Gram-stain-variable	73:91	arg1	strain					150:155	A Gram-stain-variable, aerobic, rod-shaped, motile and spore-forming bacterial strain	71:155	A Gram-stain-variable, aerobic, rod-shaped, motile and spore-forming bacterial strain	71:155	A Gram-stain-variable, aerobic, rod-shaped, motile and spore-forming bacterial strain, designated CJ11T, was isolated from a tidal flat sediment sample from Ganghwa-do, Republic of Korea.
29458566	2	53	theme	motile	115:120	arg1	strain					150:155	A Gram-stain-variable, aerobic, rod-shaped, motile and spore-forming bacterial strain	71:155	A Gram-stain-variable, aerobic, rod-shaped, motile and spore-forming bacterial strain	71:155	A Gram-stain-variable, aerobic, rod-shaped, motile and spore-forming bacterial strain, designated CJ11T, was isolated from a tidal flat sediment sample from Ganghwa-do, Republic of Korea.
29458566	10	54	theme	fatty	1001:1005	arg1	anteiso-C15 					1018:1029	anteiso-C15 	1018:1029	anteiso-C15 	1018:1029	The major cellular fatty acids were anteiso-C15 : 0, C16 : 0 and iso-C16 : 0.
29458566	10	54	theme	fatty	1001:1005	arg1	acids					1007:1011	The major cellular fatty acids	982:1011	The major cellular fatty acids	982:1011	The major cellular fatty acids were anteiso-C15 : 0, C16 : 0 and iso-C16 : 0.
29458566	2	55	theme	tidal	196:200	arg1	sample					216:221	a tidal flat sediment sample	194:221	a tidal flat sediment sample from Ganghwa-do, Republic of Korea	194:256	A Gram-stain-variable, aerobic, rod-shaped, motile and spore-forming bacterial strain, designated CJ11T, was isolated from a tidal flat sediment sample from Ganghwa-do, Republic of Korea.
29458566	4	56	theme	Sequencing	315:324	arg1	results					326:332	Sequencing results	315:332	Sequencing results of the 16S rRNA gene	315:353	Sequencing results of the 16S rRNA gene revealed that strain CJ11T possesses two copies of the 16S rRNA gene varying at five nucleotide positions.
29458566	6	57	theme	P.	783:784	arg1	KCTC					792:795	P. lacus KCTC 33691T	783:802	P. lacus KCTC 33691T	783:802	DNA-DNA relatedness levels of strain CJ11T was 41.7 % (reciprocal, 57.8 %) to P. lacus KCTC 33691T.
29458566	7	58	theme	genomic	828:834	arg1	DNA					836:838	the genomic DNA	824:838	the genomic DNA	824:838	The G+C content of the genomic DNA was 51.0 mol%.
29458566	10	59	dep	anteiso-C15 	1018:1029	arg1	iso-C16 					1047:1054	iso-C16 	1047:1054	iso-C16 	1047:1054	The major cellular fatty acids were anteiso-C15 : 0, C16 : 0 and iso-C16 : 0.
29458566	10	59	dep	anteiso-C15 	1018:1029	arg1	 0					1040:1041	 0	1040:1041	 0	1040:1041	The major cellular fatty acids were anteiso-C15 : 0, C16 : 0 and iso-C16 : 0.
29458566	10	59	dep	anteiso-C15 	1018:1029	arg1	C16 					1035:1038	C16 	1035:1038	C16 	1035:1038	The major cellular fatty acids were anteiso-C15 : 0, C16 : 0 and iso-C16 : 0.
29458566	10	59	dep	anteiso-C15 	1018:1029	arg1	 0					1056:1057	 0	1056:1057	 0	1056:1057	The major cellular fatty acids were anteiso-C15 : 0, C16 : 0 and iso-C16 : 0.
29458566	4	60	theme	strain	369:374	arg1	CJ11T					376:380	strain CJ11T	369:380	strain CJ11T	369:380	Sequencing results of the 16S rRNA gene revealed that strain CJ11T possesses two copies of the 16S rRNA gene varying at five nucleotide positions.
29458566	12	61	theme	Paenibacillus	1359:1371	arg1	genus					1353:1357	the genus Paenibacillus	1349:1371	the genus Paenibacillus	1349:1371	On the basis of the polyphasic taxonomic study, strain CJ11T represents a novel species in the genus Paenibacillus, for which the name Paenibacillustranslucens sp.
29458566	12	62	theme	Paenibacillustranslucens	1393:1416	arg1	sp					1418:1419	the name Paenibacillustranslucens sp	1384:1419	the name Paenibacillustranslucens sp	1384:1419	On the basis of the polyphasic taxonomic study, strain CJ11T represents a novel species in the genus Paenibacillus, for which the name Paenibacillustranslucens sp.
29458566	7	63	theme	DNA	836:838	arg1	%					852:852	51.0 mol%	844:852	51.0 mol%	844:852	The G+C content of the genomic DNA was 51.0 mol%.
29458566	7	63	theme	DNA	836:838	arg1	G+C content					809:819	The G+C content	805:819	The G+C content of the genomic DNA	805:838	The G+C content of the genomic DNA was 51.0 mol%.
29458566	5	64	theme	Paenibacillus	645:657	arg1	 similarity					691:701	99.36-99.15 % similarity	678:701	99.36-99.15 % similarity	678:701	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CJ11T belonged to the genus Paenibacillus within the family Paenibacillaceae and was most closely related to Paenibacillus lacus KCTC 33691T (99.36-99.15 % similarity).
29458566	5	64	theme	Paenibacillus	645:657	arg1	33691T					670:675	Paenibacillus lacus KCTC 33691T	645:675	Paenibacillus lacus KCTC 33691T (99.36-99.15 % similarity)	645:702	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CJ11T belonged to the genus Paenibacillus within the family Paenibacillaceae and was most closely related to Paenibacillus lacus KCTC 33691T (99.36-99.15 % similarity).
29458566	10	65	theme	major	986:990	arg1	anteiso-C15 					1018:1029	anteiso-C15 	1018:1029	anteiso-C15 	1018:1029	The major cellular fatty acids were anteiso-C15 : 0, C16 : 0 and iso-C16 : 0.
29458566	10	65	theme	major	986:990	arg1	acids					1007:1011	The major cellular fatty acids	982:1011	The major cellular fatty acids	982:1011	The major cellular fatty acids were anteiso-C15 : 0, C16 : 0 and iso-C16 : 0.
29458566	0	66	dep	sp	26:27	arg1	translucens					14:24	Paenibacillus translucens	0:24	Paenibacillus translucens	0:24	Paenibacillus translucens sp.
29458566	5	67	theme	Phylogenetic	462:473	arg1	analysis					475:482	Phylogenetic analysis	462:482	Phylogenetic analysis based on 16S rRNA gene sequences	462:515	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CJ11T belonged to the genus Paenibacillus within the family Paenibacillaceae and was most closely related to Paenibacillus lacus KCTC 33691T (99.36-99.15 % similarity).
29458566	14	68	theme	type	1444:1447	arg1	CJ11T					1459:1463	CJ11T	1459:1463	CJ11T (=KACC 19304T=JCM 32080T)	1459:1489	The type strain is CJ11T (=KACC 19304T=JCM 32080T).
29458566	14	68	theme	type	1444:1447	arg1	strain					1449:1454	The type strain	1440:1454	The type strain	1440:1454	The type strain is CJ11T (=KACC 19304T=JCM 32080T).
29458566	6	69	theme	lacus	786:790	arg1	KCTC					792:795	P. lacus KCTC 33691T	783:802	P. lacus KCTC 33691T	783:802	DNA-DNA relatedness levels of strain CJ11T was 41.7 % (reciprocal, 57.8 %) to P. lacus KCTC 33691T.
29458566	12	70	theme	strain	1306:1311	arg1	CJ11T					1313:1317	strain CJ11T	1306:1317	strain CJ11T	1306:1317	On the basis of the polyphasic taxonomic study, strain CJ11T represents a novel species in the genus Paenibacillus, for which the name Paenibacillustranslucens sp.
29458566	12	71	theme	taxonomic	1289:1297	arg1	study					1299:1303	the polyphasic taxonomic study	1274:1303	the polyphasic taxonomic study	1274:1303	On the basis of the polyphasic taxonomic study, strain CJ11T represents a novel species in the genus Paenibacillus, for which the name Paenibacillustranslucens sp.
29458566	4	72	theme	nucleotide	440:449	arg1	positions					451:459	five nucleotide positions	435:459	five nucleotide positions	435:459	Sequencing results of the 16S rRNA gene revealed that strain CJ11T possesses two copies of the 16S rRNA gene varying at five nucleotide positions.
29458566	12	73	theme	polyphasic	1278:1287	arg1	study					1299:1303	the polyphasic taxonomic study	1274:1303	the polyphasic taxonomic study	1274:1303	On the basis of the polyphasic taxonomic study, strain CJ11T represents a novel species in the genus Paenibacillus, for which the name Paenibacillustranslucens sp.
29458497	5	0	theme	closest	602:608	arg1	12702T					665:670	Sporichthya polymorpha NBRC 12702T	637:670	Sporichthya polymorpha NBRC 12702T with 93.7 % 16S rRNA gene sequence similarity	637:716	The closest phylogenetic neighbour was Sporichthya polymorpha NBRC 12702T with 93.7 % 16S rRNA gene sequence similarity.
29458497	5	0	theme	closest	602:608	arg1	neighbour					623:631	The closest phylogenetic neighbour	598:631	The closest phylogenetic neighbour	598:631	The closest phylogenetic neighbour was Sporichthya polymorpha NBRC 12702T with 93.7 % 16S rRNA gene sequence similarity.
29458497	8	1	theme	unidentified	925:936	arg1	menaquinone					938:948	an unidentified menaquinone	922:948	an unidentified menaquinone	922:948	The organism contained menaquinone MK-8(H2), MK-9(H4) and an unidentified menaquinone.
29458497	4	2	theme	distinct	545:552	arg1	clade					554:558	a distinct clade	543:558	a distinct clade	543:558	Phylogenetic analysis based on the 16S rRNA gene sequences revealed that the organism was closely related to the genus Sporichthya and consistently formed a distinct clade along with the members of this genus.
29458497	0	3	theme	gen.	65:68	arg1	genus					8:12	A novel genus	0:12	A novel genus of the class Actinobacteria	0:40	A novel genus of the class Actinobacteria, Longivirga aurantiaca gen. nov., sp.
29458497	0	3	theme	gen.	65:68	arg1	nov.					70:73	Longivirga aurantiaca gen. nov.	43:73	Longivirga aurantiaca gen. nov.	43:73	A novel genus of the class Actinobacteria, Longivirga aurantiaca gen. nov., sp.
29458497	5	4	theme	phylogenetic	610:621	arg1	12702T					665:670	Sporichthya polymorpha NBRC 12702T	637:670	Sporichthya polymorpha NBRC 12702T with 93.7 % 16S rRNA gene sequence similarity	637:716	The closest phylogenetic neighbour was Sporichthya polymorpha NBRC 12702T with 93.7 % 16S rRNA gene sequence similarity.
29458497	5	4	theme	phylogenetic	610:621	arg1	neighbour					623:631	The closest phylogenetic neighbour	598:631	The closest phylogenetic neighbour	598:631	The closest phylogenetic neighbour was Sporichthya polymorpha NBRC 12702T with 93.7 % 16S rRNA gene sequence similarity.
29458497	9	5	theme	unidentified	1006:1017	arg1	phospholipids					1043:1055	two unidentified phospholipids	1026:1055	two unidentified phospholipids	1026:1055	Polar lipids were composed of phosphatidylglycerol, an unidentified lipid, two unidentified phospholipids and two unidentified aminolipids.
29458497	9	5	theme	unidentified	1006:1017	arg1	phosphatidylglycerol					981:1000	phosphatidylglycerol	981:1000	phosphatidylglycerol	981:1000	Polar lipids were composed of phosphatidylglycerol, an unidentified lipid, two unidentified phospholipids and two unidentified aminolipids.
29458497	9	5	theme	unidentified	1006:1017	arg1	lipid					1019:1023	an unidentified lipid	1003:1023	an unidentified lipid	1003:1023	Polar lipids were composed of phosphatidylglycerol, an unidentified lipid, two unidentified phospholipids and two unidentified aminolipids.
29458497	9	5	theme	unidentified	1006:1017	arg1	aminolipids					1078:1088	two unidentified aminolipids	1061:1088	two unidentified aminolipids	1061:1088	Polar lipids were composed of phosphatidylglycerol, an unidentified lipid, two unidentified phospholipids and two unidentified aminolipids.
29458497	2	6	theme	actinobacterial	123:137	arg1	strain					139:144	A novel actinobacterial strain	115:144	A novel actinobacterial strain	115:144	A novel actinobacterial strain, designated X5T, was isolated from the sediment of Taihu Lake in China and was subjected to a polyphasic taxonomic characterization.
29458497	8	7	theme	menaquinone	887:897	arg1	MK-8					899:902	menaquinone MK-8	887:902	menaquinone MK-8(H2)	887:906	The organism contained menaquinone MK-8(H2), MK-9(H4) and an unidentified menaquinone.
29458497	8	7	theme	menaquinone	887:897	arg1	H2					904:905	H2	904:905	H2	904:905	The organism contained menaquinone MK-8(H2), MK-9(H4) and an unidentified menaquinone.
29458497	5	8	theme	rRNA	688:691	arg1	similarity					707:716	93.7 % 16S rRNA gene sequence similarity	677:716	93.7 % 16S rRNA gene sequence similarity	677:716	The closest phylogenetic neighbour was Sporichthya polymorpha NBRC 12702T with 93.7 % 16S rRNA gene sequence similarity.
29458497	14	9	theme	4.7317T=NBRC	1468:1479	arg1	112237T					1481:1487	=CGMCC 4.7317T=NBRC 112237T	1461:1487	=CGMCC 4.7317T=NBRC 112237T	1461:1487	The type strain is X5T (=CGMCC 4.7317T=NBRC 112237T).
29458497	14	9	theme	4.7317T=NBRC	1468:1479	arg1	X5T					1456:1458	X5T	1456:1458	X5T (=CGMCC 4.7317T=NBRC 112237T)	1456:1488	The type strain is X5T (=CGMCC 4.7317T=NBRC 112237T).
29458497	7	10	theme	genomic	825:831	arg1	G+C content					837:847	The genomic DNA G+C content	821:847	The genomic DNA G+C content	821:847	The genomic DNA G+C content was 74.4 mol%.
29458497	7	10	theme	genomic	825:831	arg1	%					861:861	74.4 mol%	853:861	74.4 mol%	853:861	The genomic DNA G+C content was 74.4 mol%.
29458497	12	11	theme	biochemical	1261:1271	arg1	data					1292:1295	the physiological, biochemical and chemotaxonomic data	1242:1295	the physiological, biochemical and chemotaxonomic data	1242:1295	Based on the physiological, biochemical and chemotaxonomic data, the organism is proposed to represent a novel genus and species, for which the name Longivirga aurantiaca gen. nov., sp.
29458497	10	12	theme	whole-cell	1095:1104	arg1	sugars					1106:1111	The whole-cell sugars	1091:1111	The whole-cell sugars	1091:1111	The whole-cell sugars contained ribose, xylose, mannose, glucose and galactose.
29458497	12	13	theme	aurantiaca	1393:1402	arg1	nov.					1409:1412	the name Longivirga aurantiaca gen. nov.	1373:1412	the name Longivirga aurantiaca gen. nov.	1373:1412	Based on the physiological, biochemical and chemotaxonomic data, the organism is proposed to represent a novel genus and species, for which the name Longivirga aurantiaca gen. nov., sp.
29458497	3	14	theme	aerobic	328:334	arg1	cells					369:373	aerobic, Gram-stain-negative, rod-shaped cells	328:373	aerobic, Gram-stain-negative, rod-shaped cells	328:373	The strain formed orange-red colonies comprising aerobic, Gram-stain-negative, rod-shaped cells on R2A agar.
29458497	2	15	theme	novel	117:121	arg1	strain					139:144	A novel actinobacterial strain	115:144	A novel actinobacterial strain	115:144	A novel actinobacterial strain, designated X5T, was isolated from the sediment of Taihu Lake in China and was subjected to a polyphasic taxonomic characterization.
29458497	12	16	theme	name	1377:1380	arg1	nov.					1409:1412	the name Longivirga aurantiaca gen. nov.	1373:1412	the name Longivirga aurantiaca gen. nov.	1373:1412	Based on the physiological, biochemical and chemotaxonomic data, the organism is proposed to represent a novel genus and species, for which the name Longivirga aurantiaca gen. nov., sp.
29458497	12	17	theme	Longivirga	1382:1391	arg1	nov.					1409:1412	the name Longivirga aurantiaca gen. nov.	1373:1412	the name Longivirga aurantiaca gen. nov.	1373:1412	Based on the physiological, biochemical and chemotaxonomic data, the organism is proposed to represent a novel genus and species, for which the name Longivirga aurantiaca gen. nov., sp.
29458497	3	18	theme	orange-red	297:306	arg1	colonies					308:315	orange-red colonies	297:315	orange-red colonies comprising aerobic, Gram-stain-negative, rod-shaped cells	297:373	The strain formed orange-red colonies comprising aerobic, Gram-stain-negative, rod-shaped cells on R2A agar.
29458497	4	19	theme	gene	432:435	arg1	sequences					437:445	the 16S rRNA gene sequences	419:445	the 16S rRNA gene sequences	419:445	Phylogenetic analysis based on the 16S rRNA gene sequences revealed that the organism was closely related to the genus Sporichthya and consistently formed a distinct clade along with the members of this genus.
29458497	0	20	dep	sp	76:77	arg1	genus					8:12	A novel genus	0:12	A novel genus of the class Actinobacteria	0:40	A novel genus of the class Actinobacteria, Longivirga aurantiaca gen. nov., sp.
29458497	0	20	dep	sp	76:77	arg1	nov.					70:73	Longivirga aurantiaca gen. nov.	43:73	Longivirga aurantiaca gen. nov.	43:73	A novel genus of the class Actinobacteria, Longivirga aurantiaca gen. nov., sp.
29458497	11	21	theme	ll-diaminopimelic	1209:1225	arg1	acid					1227:1230	ll-diaminopimelic acid	1209:1230	ll-diaminopimelic acid	1209:1230	The cell-wall peptidoglycan contained ll-diaminopimelic acid.
29458497	3	22	theme	R2A	378:380	arg1	agar					382:385	R2A agar	378:385	R2A agar	378:385	The strain formed orange-red colonies comprising aerobic, Gram-stain-negative, rod-shaped cells on R2A agar.
29458497	9	23	theme	unidentified	1030:1041	arg1	phospholipids					1043:1055	two unidentified phospholipids	1026:1055	two unidentified phospholipids	1026:1055	Polar lipids were composed of phosphatidylglycerol, an unidentified lipid, two unidentified phospholipids and two unidentified aminolipids.
29458497	9	23	theme	unidentified	1030:1041	arg1	lipid					1019:1023	an unidentified lipid	1003:1023	an unidentified lipid	1003:1023	Polar lipids were composed of phosphatidylglycerol, an unidentified lipid, two unidentified phospholipids and two unidentified aminolipids.
29458497	12	24	dep	sp	1415:1416	arg1	nov.					1409:1412	the name Longivirga aurantiaca gen. nov.	1373:1412	the name Longivirga aurantiaca gen. nov.	1373:1412	Based on the physiological, biochemical and chemotaxonomic data, the organism is proposed to represent a novel genus and species, for which the name Longivirga aurantiaca gen. nov., sp.
29458497	2	25	attach	isolated	167:174	arg1	sediment					185:192	the sediment	181:192	the sediment of Taihu Lake in China	181:215	A novel actinobacterial strain, designated X5T, was isolated from the sediment of Taihu Lake in China and was subjected to a polyphasic taxonomic characterization.
29458497	2	25	attach	isolated	167:174	arg2	strain					139:144	A novel actinobacterial strain	115:144	A novel actinobacterial strain	115:144	A novel actinobacterial strain, designated X5T, was isolated from the sediment of Taihu Lake in China and was subjected to a polyphasic taxonomic characterization.
29458497	0	26	theme	novel	2:6	arg1	genus					8:12	A novel genus	0:12	A novel genus of the class Actinobacteria	0:40	A novel genus of the class Actinobacteria, Longivirga aurantiaca gen. nov., sp.
29458497	0	26	theme	novel	2:6	arg1	nov.					70:73	Longivirga aurantiaca gen. nov.	43:73	Longivirga aurantiaca gen. nov.	43:73	A novel genus of the class Actinobacteria, Longivirga aurantiaca gen. nov., sp.
29458497	2	27	from	sediment	185:192	arg1	China					211:215	China	211:215	China	211:215	A novel actinobacterial strain, designated X5T, was isolated from the sediment of Taihu Lake in China and was subjected to a polyphasic taxonomic characterization.
29458497	12	28	theme	novel	1338:1342	arg1	genus					1344:1348	a novel genus	1336:1348	a novel genus	1336:1348	Based on the physiological, biochemical and chemotaxonomic data, the organism is proposed to represent a novel genus and species, for which the name Longivirga aurantiaca gen. nov., sp.
29458497	2	29	theme	taxonomic	251:259	arg1	characterization					261:276	a polyphasic taxonomic characterization	238:276	a polyphasic taxonomic characterization	238:276	A novel actinobacterial strain, designated X5T, was isolated from the sediment of Taihu Lake in China and was subjected to a polyphasic taxonomic characterization.
29458497	2	30	theme	polyphasic	240:249	arg1	characterization					261:276	a polyphasic taxonomic characterization	238:276	a polyphasic taxonomic characterization	238:276	A novel actinobacterial strain, designated X5T, was isolated from the sediment of Taihu Lake in China and was subjected to a polyphasic taxonomic characterization.
29458497	14	31	theme	=CGMCC	1461:1466	arg1	112237T					1481:1487	=CGMCC 4.7317T=NBRC 112237T	1461:1487	=CGMCC 4.7317T=NBRC 112237T	1461:1487	The type strain is X5T (=CGMCC 4.7317T=NBRC 112237T).
29458497	14	31	theme	=CGMCC	1461:1466	arg1	X5T					1456:1458	X5T	1456:1458	X5T (=CGMCC 4.7317T=NBRC 112237T)	1456:1488	The type strain is X5T (=CGMCC 4.7317T=NBRC 112237T).
29458497	4	32	theme	genus	591:595	arg1	members					575:581	the members	571:581	the members of this genus	571:595	Phylogenetic analysis based on the 16S rRNA gene sequences revealed that the organism was closely related to the genus Sporichthya and consistently formed a distinct clade along with the members of this genus.
29458497	5	33	theme	93.7 	677:681	arg1	%					682:682	%	682:682	%	682:682	The closest phylogenetic neighbour was Sporichthya polymorpha NBRC 12702T with 93.7 % 16S rRNA gene sequence similarity.
29458497	9	34	theme	unidentified	1065:1076	arg1	aminolipids					1078:1088	two unidentified aminolipids	1061:1088	two unidentified aminolipids	1061:1088	Polar lipids were composed of phosphatidylglycerol, an unidentified lipid, two unidentified phospholipids and two unidentified aminolipids.
29458497	9	34	theme	unidentified	1065:1076	arg1	lipid					1019:1023	an unidentified lipid	1003:1023	an unidentified lipid	1003:1023	Polar lipids were composed of phosphatidylglycerol, an unidentified lipid, two unidentified phospholipids and two unidentified aminolipids.
29458497	0	35	theme	class	21:25	arg1	Actinobacteria					27:40	the class Actinobacteria	17:40	the class Actinobacteria	17:40	A novel genus of the class Actinobacteria, Longivirga aurantiaca gen. nov., sp.
29458497	5	36	theme	%	682:682	arg1	similarity					707:716	93.7 % 16S rRNA gene sequence similarity	677:716	93.7 % 16S rRNA gene sequence similarity	677:716	The closest phylogenetic neighbour was Sporichthya polymorpha NBRC 12702T with 93.7 % 16S rRNA gene sequence similarity.
29458497	6	37	dep	iso-C16 	754:761	arg1	 0					763:764	 0	763:764	 0	763:764	The major fatty acids (>10 %) were iso-C16 : 0 (18.7 %), C18 : 1ω9c (18.6 %) and C17 : 1ω8c (14.0 %).
29458497	6	37	dep	iso-C16 	754:761	arg1	C17 					800:803	C17 	800:803	C17 	800:803	The major fatty acids (>10 %) were iso-C16 : 0 (18.7 %), C18 : 1ω9c (18.6 %) and C17 : 1ω8c (14.0 %).
29458497	6	37	dep	iso-C16 	754:761	arg1	%					772:772	18.7 %	767:772	18.7 %	767:772	The major fatty acids (>10 %) were iso-C16 : 0 (18.7 %), C18 : 1ω9c (18.6 %) and C17 : 1ω8c (14.0 %).
29458497	6	37	dep	iso-C16 	754:761	arg1	 1ω8c					805:809	 1ω8c	805:809	 1ω8c	805:809	The major fatty acids (>10 %) were iso-C16 : 0 (18.7 %), C18 : 1ω9c (18.6 %) and C17 : 1ω8c (14.0 %).
29458497	6	37	dep	iso-C16 	754:761	arg1	%					817:817	14.0 %	812:817	14.0 %	812:817	The major fatty acids (>10 %) were iso-C16 : 0 (18.7 %), C18 : 1ω9c (18.6 %) and C17 : 1ω8c (14.0 %).
29458497	6	37	dep	iso-C16 	754:761	arg1	C18 					776:779	C18 	776:779	C18 	776:779	The major fatty acids (>10 %) were iso-C16 : 0 (18.7 %), C18 : 1ω9c (18.6 %) and C17 : 1ω8c (14.0 %).
29458497	8	38	contain	contained	877:885	arg2	H2					904:905	H2	904:905	H2	904:905	The organism contained menaquinone MK-8(H2), MK-9(H4) and an unidentified menaquinone.
29458497	8	38	contain	contained	877:885	arg2	MK-9					909:912	MK-9	909:912	MK-9(H4)	909:916	The organism contained menaquinone MK-8(H2), MK-9(H4) and an unidentified menaquinone.
29458497	8	38	contain	contained	877:885	arg2	menaquinone					938:948	an unidentified menaquinone	922:948	an unidentified menaquinone	922:948	The organism contained menaquinone MK-8(H2), MK-9(H4) and an unidentified menaquinone.
29458497	8	38	contain	contained	877:885	arg1	organism					868:875	The organism	864:875	The organism	864:875	The organism contained menaquinone MK-8(H2), MK-9(H4) and an unidentified menaquinone.
29458497	8	38	contain	contained	877:885	arg2	H4					914:915	H4	914:915	H4	914:915	The organism contained menaquinone MK-8(H2), MK-9(H4) and an unidentified menaquinone.
29458497	8	38	contain	contained	877:885	arg2	MK-8					899:902	menaquinone MK-8	887:902	menaquinone MK-8(H2)	887:906	The organism contained menaquinone MK-8(H2), MK-9(H4) and an unidentified menaquinone.
29458497	5	39	theme	16S	684:686	arg1	similarity					707:716	93.7 % 16S rRNA gene sequence similarity	677:716	93.7 % 16S rRNA gene sequence similarity	677:716	The closest phylogenetic neighbour was Sporichthya polymorpha NBRC 12702T with 93.7 % 16S rRNA gene sequence similarity.
29458497	4	40	theme	rRNA	427:430	arg1	sequences					437:445	the 16S rRNA gene sequences	419:445	the 16S rRNA gene sequences	419:445	Phylogenetic analysis based on the 16S rRNA gene sequences revealed that the organism was closely related to the genus Sporichthya and consistently formed a distinct clade along with the members of this genus.
29458497	11	41	contain	contained	1199:1207	arg2	acid					1227:1230	ll-diaminopimelic acid	1209:1230	ll-diaminopimelic acid	1209:1230	The cell-wall peptidoglycan contained ll-diaminopimelic acid.
29458497	11	41	contain	contained	1199:1207	arg1	peptidoglycan					1185:1197	The cell-wall peptidoglycan	1171:1197	The cell-wall peptidoglycan	1171:1197	The cell-wall peptidoglycan contained ll-diaminopimelic acid.
29458497	6	42	theme	fatty	729:733	arg1	acids					735:739	The major fatty acids	719:739	The major fatty acids (>10 %)	719:747	The major fatty acids (>10 %) were iso-C16 : 0 (18.7 %), C18 : 1ω9c (18.6 %) and C17 : 1ω8c (14.0 %).
29458497	6	42	theme	fatty	729:733	arg1	%					746:746	>10 %	742:746	>10 %	742:746	The major fatty acids (>10 %) were iso-C16 : 0 (18.7 %), C18 : 1ω9c (18.6 %) and C17 : 1ω8c (14.0 %).
29458497	6	42	theme	fatty	729:733	arg1	iso-C16 					754:761	iso-C16 	754:761	iso-C16 	754:761	The major fatty acids (>10 %) were iso-C16 : 0 (18.7 %), C18 : 1ω9c (18.6 %) and C17 : 1ω8c (14.0 %).
29458497	5	43	theme	Sporichthya	637:647	arg1	12702T					665:670	Sporichthya polymorpha NBRC 12702T	637:670	Sporichthya polymorpha NBRC 12702T with 93.7 % 16S rRNA gene sequence similarity	637:716	The closest phylogenetic neighbour was Sporichthya polymorpha NBRC 12702T with 93.7 % 16S rRNA gene sequence similarity.
29458497	5	43	theme	Sporichthya	637:647	arg1	neighbour					623:631	The closest phylogenetic neighbour	598:631	The closest phylogenetic neighbour	598:631	The closest phylogenetic neighbour was Sporichthya polymorpha NBRC 12702T with 93.7 % 16S rRNA gene sequence similarity.
29458497	4	44	theme	16S	423:425	arg1	sequences					437:445	the 16S rRNA gene sequences	419:445	the 16S rRNA gene sequences	419:445	Phylogenetic analysis based on the 16S rRNA gene sequences revealed that the organism was closely related to the genus Sporichthya and consistently formed a distinct clade along with the members of this genus.
29458497	6	45	theme	major	723:727	arg1	acids					735:739	The major fatty acids	719:739	The major fatty acids (>10 %)	719:747	The major fatty acids (>10 %) were iso-C16 : 0 (18.7 %), C18 : 1ω9c (18.6 %) and C17 : 1ω8c (14.0 %).
29458497	6	45	theme	major	723:727	arg1	%					746:746	>10 %	742:746	>10 %	742:746	The major fatty acids (>10 %) were iso-C16 : 0 (18.7 %), C18 : 1ω9c (18.6 %) and C17 : 1ω8c (14.0 %).
29458497	6	45	theme	major	723:727	arg1	iso-C16 					754:761	iso-C16 	754:761	iso-C16 	754:761	The major fatty acids (>10 %) were iso-C16 : 0 (18.7 %), C18 : 1ω9c (18.6 %) and C17 : 1ω8c (14.0 %).
29458497	0	46	theme	Actinobacteria	27:40	arg1	genus					8:12	A novel genus	0:12	A novel genus of the class Actinobacteria	0:40	A novel genus of the class Actinobacteria, Longivirga aurantiaca gen. nov., sp.
29458497	0	46	theme	Actinobacteria	27:40	arg1	nov.					70:73	Longivirga aurantiaca gen. nov.	43:73	Longivirga aurantiaca gen. nov.	43:73	A novel genus of the class Actinobacteria, Longivirga aurantiaca gen. nov., sp.
29458497	4	47	theme	genus	501:505	arg1	Sporichthya					507:517	the genus Sporichthya	497:517	the genus Sporichthya	497:517	Phylogenetic analysis based on the 16S rRNA gene sequences revealed that the organism was closely related to the genus Sporichthya and consistently formed a distinct clade along with the members of this genus.
29458497	5	48	theme	polymorpha	649:658	arg1	12702T					665:670	Sporichthya polymorpha NBRC 12702T	637:670	Sporichthya polymorpha NBRC 12702T with 93.7 % 16S rRNA gene sequence similarity	637:716	The closest phylogenetic neighbour was Sporichthya polymorpha NBRC 12702T with 93.7 % 16S rRNA gene sequence similarity.
29458497	5	48	theme	polymorpha	649:658	arg1	neighbour					623:631	The closest phylogenetic neighbour	598:631	The closest phylogenetic neighbour	598:631	The closest phylogenetic neighbour was Sporichthya polymorpha NBRC 12702T with 93.7 % 16S rRNA gene sequence similarity.
29458497	4	49	theme	Phylogenetic	388:399	arg1	analysis					401:408	Phylogenetic analysis	388:408	Phylogenetic analysis based on the 16S rRNA gene sequences	388:445	Phylogenetic analysis based on the 16S rRNA gene sequences revealed that the organism was closely related to the genus Sporichthya and consistently formed a distinct clade along with the members of this genus.
29458497	14	50	theme	type	1441:1444	arg1	X5T					1456:1458	X5T	1456:1458	X5T (=CGMCC 4.7317T=NBRC 112237T)	1456:1488	The type strain is X5T (=CGMCC 4.7317T=NBRC 112237T).
29458497	14	50	theme	type	1441:1444	arg1	strain					1446:1451	The type strain	1437:1451	The type strain	1437:1451	The type strain is X5T (=CGMCC 4.7317T=NBRC 112237T).
29458497	6	51	dep	C18 	776:779	arg1	 1ω9c					781:785	 1ω9c	781:785	 1ω9c	781:785	The major fatty acids (>10 %) were iso-C16 : 0 (18.7 %), C18 : 1ω9c (18.6 %) and C17 : 1ω8c (14.0 %).
29458497	6	51	dep	C18 	776:779	arg1	%					793:793	18.6 %	788:793	18.6 %	788:793	The major fatty acids (>10 %) were iso-C16 : 0 (18.7 %), C18 : 1ω9c (18.6 %) and C17 : 1ω8c (14.0 %).
29458497	5	52	theme	NBRC	660:663	arg1	12702T					665:670	Sporichthya polymorpha NBRC 12702T	637:670	Sporichthya polymorpha NBRC 12702T with 93.7 % 16S rRNA gene sequence similarity	637:716	The closest phylogenetic neighbour was Sporichthya polymorpha NBRC 12702T with 93.7 % 16S rRNA gene sequence similarity.
29458497	5	52	theme	NBRC	660:663	arg1	neighbour					623:631	The closest phylogenetic neighbour	598:631	The closest phylogenetic neighbour	598:631	The closest phylogenetic neighbour was Sporichthya polymorpha NBRC 12702T with 93.7 % 16S rRNA gene sequence similarity.
29458497	12	53	theme	chemotaxonomic	1277:1290	arg1	data					1292:1295	the physiological, biochemical and chemotaxonomic data	1242:1295	the physiological, biochemical and chemotaxonomic data	1242:1295	Based on the physiological, biochemical and chemotaxonomic data, the organism is proposed to represent a novel genus and species, for which the name Longivirga aurantiaca gen. nov., sp.
29458497	12	54	theme	physiological	1246:1258	arg1	data					1292:1295	the physiological, biochemical and chemotaxonomic data	1242:1295	the physiological, biochemical and chemotaxonomic data	1242:1295	Based on the physiological, biochemical and chemotaxonomic data, the organism is proposed to represent a novel genus and species, for which the name Longivirga aurantiaca gen. nov., sp.
29458497	5	55	theme	sequence	698:705	arg1	similarity					707:716	93.7 % 16S rRNA gene sequence similarity	677:716	93.7 % 16S rRNA gene sequence similarity	677:716	The closest phylogenetic neighbour was Sporichthya polymorpha NBRC 12702T with 93.7 % 16S rRNA gene sequence similarity.
29458497	3	56	dep	aerobic	328:334	arg1	rod-shaped					358:367	rod-shaped	358:367	rod-shaped	358:367	The strain formed orange-red colonies comprising aerobic, Gram-stain-negative, rod-shaped cells on R2A agar.
29458497	3	56	dep	aerobic	328:334	arg1	Gram-stain-negative					337:355	Gram-stain-negative	337:355	Gram-stain-negative	337:355	The strain formed orange-red colonies comprising aerobic, Gram-stain-negative, rod-shaped cells on R2A agar.
29458497	10	57	contain	contained	1113:1121	arg2	mannose					1139:1145	mannose	1139:1145	mannose	1139:1145	The whole-cell sugars contained ribose, xylose, mannose, glucose and galactose.
29458497	10	57	contain	contained	1113:1121	arg2	ribose					1123:1128	ribose	1123:1128	ribose	1123:1128	The whole-cell sugars contained ribose, xylose, mannose, glucose and galactose.
29458497	10	57	contain	contained	1113:1121	arg2	glucose					1148:1154	glucose	1148:1154	glucose	1148:1154	The whole-cell sugars contained ribose, xylose, mannose, glucose and galactose.
29458497	10	57	contain	contained	1113:1121	arg2	galactose					1160:1168	galactose	1160:1168	galactose	1160:1168	The whole-cell sugars contained ribose, xylose, mannose, glucose and galactose.
29458497	10	57	contain	contained	1113:1121	arg1	sugars					1106:1111	The whole-cell sugars	1091:1111	The whole-cell sugars	1091:1111	The whole-cell sugars contained ribose, xylose, mannose, glucose and galactose.
29458497	10	57	contain	contained	1113:1121	arg2	xylose					1131:1136	xylose	1131:1136	xylose	1131:1136	The whole-cell sugars contained ribose, xylose, mannose, glucose and galactose.
29458497	9	58	theme	Polar	951:955	arg1	lipids					957:962	Polar lipids	951:962	Polar lipids	951:962	Polar lipids were composed of phosphatidylglycerol, an unidentified lipid, two unidentified phospholipids and two unidentified aminolipids.
29458497	1	59	theme	lake	100:103	arg1	sediment					105:112	lake sediment	100:112	lake sediment	100:112	nov., isolated from lake sediment.
29458497	12	60	theme	gen.	1404:1407	arg1	nov.					1409:1412	the name Longivirga aurantiaca gen. nov.	1373:1412	the name Longivirga aurantiaca gen. nov.	1373:1412	Based on the physiological, biochemical and chemotaxonomic data, the organism is proposed to represent a novel genus and species, for which the name Longivirga aurantiaca gen. nov., sp.
29458497	0	61	theme	aurantiaca	54:63	arg1	genus					8:12	A novel genus	0:12	A novel genus of the class Actinobacteria	0:40	A novel genus of the class Actinobacteria, Longivirga aurantiaca gen. nov., sp.
29458497	0	61	theme	aurantiaca	54:63	arg1	nov.					70:73	Longivirga aurantiaca gen. nov.	43:73	Longivirga aurantiaca gen. nov.	43:73	A novel genus of the class Actinobacteria, Longivirga aurantiaca gen. nov., sp.
29458497	2	62	theme	Lake	203:206	arg1	sediment					185:192	the sediment	181:192	the sediment of Taihu Lake in China	181:215	A novel actinobacterial strain, designated X5T, was isolated from the sediment of Taihu Lake in China and was subjected to a polyphasic taxonomic characterization.
29458497	7	63	theme	74.4 mol	853:860	arg1	G+C content					837:847	The genomic DNA G+C content	821:847	The genomic DNA G+C content	821:847	The genomic DNA G+C content was 74.4 mol%.
29458497	7	63	theme	74.4 mol	853:860	arg1	%					861:861	74.4 mol%	853:861	74.4 mol%	853:861	The genomic DNA G+C content was 74.4 mol%.
29458497	5	64	with	12702T	665:670	arg1	similarity					707:716	93.7 % 16S rRNA gene sequence similarity	677:716	93.7 % 16S rRNA gene sequence similarity	677:716	The closest phylogenetic neighbour was Sporichthya polymorpha NBRC 12702T with 93.7 % 16S rRNA gene sequence similarity.
29458497	0	65	theme	Longivirga	43:52	arg1	genus					8:12	A novel genus	0:12	A novel genus of the class Actinobacteria	0:40	A novel genus of the class Actinobacteria, Longivirga aurantiaca gen. nov., sp.
29458497	0	65	theme	Longivirga	43:52	arg1	nov.					70:73	Longivirga aurantiaca gen. nov.	43:73	Longivirga aurantiaca gen. nov.	43:73	A novel genus of the class Actinobacteria, Longivirga aurantiaca gen. nov., sp.
29458497	7	66	theme	DNA	833:835	arg1	G+C content					837:847	The genomic DNA G+C content	821:847	The genomic DNA G+C content	821:847	The genomic DNA G+C content was 74.4 mol%.
29458497	7	66	theme	DNA	833:835	arg1	%					861:861	74.4 mol%	853:861	74.4 mol%	853:861	The genomic DNA G+C content was 74.4 mol%.
29458497	11	67	theme	cell-wall	1175:1183	arg1	peptidoglycan					1185:1197	The cell-wall peptidoglycan	1171:1197	The cell-wall peptidoglycan	1171:1197	The cell-wall peptidoglycan contained ll-diaminopimelic acid.
29458497	5	68	theme	gene	693:696	arg1	similarity					707:716	93.7 % 16S rRNA gene sequence similarity	677:716	93.7 % 16S rRNA gene sequence similarity	677:716	The closest phylogenetic neighbour was Sporichthya polymorpha NBRC 12702T with 93.7 % 16S rRNA gene sequence similarity.
31081119	3	0	theme	obesity	1043:1049	arg1	risk					1035:1038	an increased risk	1022:1038	an increased risk of obesity in the offspring	1022:1066	These data suggest that maternal intestinal changes probably contribute to adverse placental adaptations and also impart an increased risk of obesity in the offspring via alterations in fetal gut development.
31081119	3	1	from	alterations	1072:1082	arg1	development					1097:1107	fetal gut development	1087:1107	fetal gut development	1087:1107	These data suggest that maternal intestinal changes probably contribute to adverse placental adaptations and also impart an increased risk of obesity in the offspring via alterations in fetal gut development.
31081119	2	2	theme	stress	885:890	arg1	markers					892:898	endoplasmic reticulum stress markers	863:898	endoplasmic reticulum stress markers	863:898	Placenta from obese mothers showed blood vessel immaturity, hypoxia, increased transcript levels of inflammation, autophagy and altered levels of endoplasmic reticulum stress markers.
31081119	9	3	theme	vessel	2165:2170	arg1	immaturity					2172:2181	blood vessel immaturity	2159:2181	blood vessel immaturity	2159:2181	Placentas from HFD dams demonstrated blood vessel immaturity and hypoxia; decreased free carnitine, acylcarnitine derivatives and trimethylamine-N-oxide; and altered mRNA levels of inflammation, autophagy, and ER stress markers.
31081119	0	4	theme	immune	160:165	arg1	markers					167:173	immune markers	160:173	immune markers	160:173	High-fat diet intake modulates maternal intestinal adaptations to pregnancy and results in placental hypoxia, as well as altered fetal gut barrier proteins and immune markers.
31081119	1	5	theme	diet-induced	433:444	arg1	obesity					446:452	diet-induced obesity	433:452	diet-induced obesity in mice	433:460	KEY POINTS Maternal obesity has been associated with shifts in intestinal microbiota, which may contribute to impaired barrier function Impaired barrier function may expose the placenta and fetus to pro-inflammatory mediators We investigated the impacts of diet-induced obesity in mice on maternal and fetal intestinal structure and placental vascularization Diet-induced obesity decreased maternal intestinal short chain fatty acids and their receptors, impaired gut barrier integrity and was associated with fetal intestinal inflammation.
31081119	1	6	theme	intestinal	239:248	arg1	microbiota					250:259	intestinal microbiota	239:259	intestinal microbiota	239:259	KEY POINTS Maternal obesity has been associated with shifts in intestinal microbiota, which may contribute to impaired barrier function Impaired barrier function may expose the placenta and fetus to pro-inflammatory mediators We investigated the impacts of diet-induced obesity in mice on maternal and fetal intestinal structure and placental vascularization Diet-induced obesity decreased maternal intestinal short chain fatty acids and their receptors, impaired gut barrier integrity and was associated with fetal intestinal inflammation.
31081119	7	7	theme	cecal	1832:1836	arg1	butyrate					1838:1845	modestly decreased cecal butyrate	1813:1845	modestly decreased cecal butyrate	1813:1845	These diet-induced shifts paralleled decreased maternal intestinal mRNA levels of SCFA receptor Gpr41, modestly decreased cecal butyrate, and altered mRNA levels of inflammatory cytokines and immune cell markers in the maternal intestine.
31081119	2	8	theme	endoplasmic	863:873	arg1	reticulum					875:883	endoplasmic reticulum	863:883	endoplasmic reticulum stress markers	863:898	Placenta from obese mothers showed blood vessel immaturity, hypoxia, increased transcript levels of inflammation, autophagy and altered levels of endoplasmic reticulum stress markers.
31081119	1	9	dep	investigated	405:416	arg1	impaired					631:638	impaired	631:638	impaired gut barrier integrity	631:660	KEY POINTS Maternal obesity has been associated with shifts in intestinal microbiota, which may contribute to impaired barrier function Impaired barrier function may expose the placenta and fetus to pro-inflammatory mediators We investigated the impacts of diet-induced obesity in mice on maternal and fetal intestinal structure and placental vascularization Diet-induced obesity decreased maternal intestinal short chain fatty acids and their receptors, impaired gut barrier integrity and was associated with fetal intestinal inflammation.
31081119	1	9	dep	investigated	405:416	arg1	associated					670:679	associated	670:679	was associated with fetal intestinal inflammation	666:714	KEY POINTS Maternal obesity has been associated with shifts in intestinal microbiota, which may contribute to impaired barrier function Impaired barrier function may expose the placenta and fetus to pro-inflammatory mediators We investigated the impacts of diet-induced obesity in mice on maternal and fetal intestinal structure and placental vascularization Diet-induced obesity decreased maternal intestinal short chain fatty acids and their receptors, impaired gut barrier integrity and was associated with fetal intestinal inflammation.
31081119	1	9	dep	investigated	405:416	arg1	decreased					556:564	decreased	556:564	decreased maternal intestinal short chain fatty acids and their receptors	556:628	KEY POINTS Maternal obesity has been associated with shifts in intestinal microbiota, which may contribute to impaired barrier function Impaired barrier function may expose the placenta and fetus to pro-inflammatory mediators We investigated the impacts of diet-induced obesity in mice on maternal and fetal intestinal structure and placental vascularization Diet-induced obesity decreased maternal intestinal short chain fatty acids and their receptors, impaired gut barrier integrity and was associated with fetal intestinal inflammation.
31081119	5	10	theme	day	1578:1580	arg1	18.5					1582:1585	embryonic day 18.5	1568:1585	embryonic day 18.5	1568:1585	In the present study, we generated cohorts of female C57BL/6J mice fed a control (17% kcal fat, n = 10-14) or a high-fat diet (HFD 60% kcal from fat, n = 10-14; ad libitum) aiming to investigate the impact on the maternal gut microbiota, intestinal inflammation and gut barrier integrity, placental inflammation and fetal intestinal development at embryonic day 18.5.
31081119	5	11	theme	maternal	1433:1440	arg1	microbiota					1446:1455	the maternal gut microbiota	1429:1455	the maternal gut microbiota	1429:1455	In the present study, we generated cohorts of female C57BL/6J mice fed a control (17% kcal fat, n = 10-14) or a high-fat diet (HFD 60% kcal from fat, n = 10-14; ad libitum) aiming to investigate the impact on the maternal gut microbiota, intestinal inflammation and gut barrier integrity, placental inflammation and fetal intestinal development at embryonic day 18.5.
31081119	10	12	theme	exposed	2355:2361	arg1	fetuses					2363:2369	HFD exposed fetuses	2351:2369	HFD exposed fetuses	2351:2369	HFD exposed fetuses had increased activation of nuclear factor-kappa B and inhibition of the unfolded protein response in the developing intestine.
31081119	4	13	theme	ABSTRACT	1110:1117	arg1	Shifts					1119:1124	ABSTRACT Shifts	1110:1124	ABSTRACT Shifts in maternal intestinal microbiota	1110:1158	ABSTRACT Shifts in maternal intestinal microbiota have been implicated in metabolic adaptations to pregnancy.
31081119	2	14	theme	transcript	796:805	arg1	immaturity					765:774	blood vessel immaturity	752:774	blood vessel immaturity	752:774	Placenta from obese mothers showed blood vessel immaturity, hypoxia, increased transcript levels of inflammation, autophagy and altered levels of endoplasmic reticulum stress markers.
31081119	2	14	theme	transcript	796:805	arg1	levels					807:812	increased transcript levels	786:812	increased transcript levels of inflammation, autophagy and altered levels of endoplasmic reticulum stress markers	786:898	Placenta from obese mothers showed blood vessel immaturity, hypoxia, increased transcript levels of inflammation, autophagy and altered levels of endoplasmic reticulum stress markers.
31081119	2	15	from	mothers	737:743	arg1	Placenta					717:724	Placenta	717:724	Placenta from obese mothers	717:743	Placenta from obese mothers showed blood vessel immaturity, hypoxia, increased transcript levels of inflammation, autophagy and altered levels of endoplasmic reticulum stress markers.
31081119	8	16	theme	maternal	2050:2057	arg1	levels					2059:2064	circulating maternal levels	2038:2064	circulating maternal levels of lipopolysaccharide (LPS) and tumour necrosis factor	2038:2119	Maternal HFD resulted in impaired gut barrier integrity, with corresponding increases in circulating maternal levels of lipopolysaccharide (LPS) and tumour necrosis factor.
31081119	7	17	theme	Gpr41	1806:1810	arg1	levels					1865:1870	altered mRNA levels	1852:1870	altered mRNA levels of inflammatory cytokines and immune cell markers in the maternal intestine	1852:1946	These diet-induced shifts paralleled decreased maternal intestinal mRNA levels of SCFA receptor Gpr41, modestly decreased cecal butyrate, and altered mRNA levels of inflammatory cytokines and immune cell markers in the maternal intestine.
31081119	7	17	theme	Gpr41	1806:1810	arg1	butyrate					1838:1845	modestly decreased cecal butyrate	1813:1845	modestly decreased cecal butyrate	1813:1845	These diet-induced shifts paralleled decreased maternal intestinal mRNA levels of SCFA receptor Gpr41, modestly decreased cecal butyrate, and altered mRNA levels of inflammatory cytokines and immune cell markers in the maternal intestine.
31081119	7	17	theme	Gpr41	1806:1810	arg1	levels					1782:1787	decreased maternal intestinal mRNA levels	1747:1787	decreased maternal intestinal mRNA levels of SCFA receptor Gpr41	1747:1810	These diet-induced shifts paralleled decreased maternal intestinal mRNA levels of SCFA receptor Gpr41, modestly decreased cecal butyrate, and altered mRNA levels of inflammatory cytokines and immune cell markers in the maternal intestine.
31081119	1	18	from	shifts	229:234	arg1	microbiota					250:259	intestinal microbiota	239:259	intestinal microbiota	239:259	KEY POINTS Maternal obesity has been associated with shifts in intestinal microbiota, which may contribute to impaired barrier function Impaired barrier function may expose the placenta and fetus to pro-inflammatory mediators We investigated the impacts of diet-induced obesity in mice on maternal and fetal intestinal structure and placental vascularization Diet-induced obesity decreased maternal intestinal short chain fatty acids and their receptors, impaired gut barrier integrity and was associated with fetal intestinal inflammation.
31081119	9	19	theme	free	2206:2209	arg1	carnitine					2211:2219	decreased free carnitine	2196:2219	decreased free carnitine	2196:2219	Placentas from HFD dams demonstrated blood vessel immaturity and hypoxia; decreased free carnitine, acylcarnitine derivatives and trimethylamine-N-oxide; and altered mRNA levels of inflammation, autophagy, and ER stress markers.
31081119	1	20	theme	KEY	176:178	arg1	POINTS					180:185	KEY POINTS	176:185	KEY POINTS Maternal obesity has been associated with shifts in intestinal microbiota, which may contribute to impaired barrier function Impaired barrier function may expose the placenta and fetus to pro-inflammatory mediators We investigated the impacts of diet-induced obesity in mice on maternal and fetal intestinal structure and placental vascularization Diet-induced obesity decreased maternal intestinal short chain fatty acids and their receptors, impaired gut barrier integrity and was associated with fetal intestinal inflammation.	176:715	KEY POINTS Maternal obesity has been associated with shifts in intestinal microbiota, which may contribute to impaired barrier function Impaired barrier function may expose the placenta and fetus to pro-inflammatory mediators We investigated the impacts of diet-induced obesity in mice on maternal and fetal intestinal structure and placental vascularization Diet-induced obesity decreased maternal intestinal short chain fatty acids and their receptors, impaired gut barrier integrity and was associated with fetal intestinal inflammation.
31081119	3	21	theme	gut	1093:1095	arg1	development					1097:1107	fetal gut development	1087:1107	fetal gut development	1087:1107	These data suggest that maternal intestinal changes probably contribute to adverse placental adaptations and also impart an increased risk of obesity in the offspring via alterations in fetal gut development.
31081119	11	22	from	changes	2751:2757	arg1	development					2802:2812	fetal gut development	2792:2812	fetal gut development	2792:2812	Taken together, these data suggest that HFD intake prior to and during pregnancy shifts the composition of the maternal gut microbiota and impairs gut barrier integrity, resulting in increased maternal circulating LPS, which may ultimate contribute to changes in placental vascularization and fetal gut development.
31081119	11	22	from	changes	2751:2757	arg1	vascularization					2772:2786	placental vascularization	2762:2786	placental vascularization	2762:2786	Taken together, these data suggest that HFD intake prior to and during pregnancy shifts the composition of the maternal gut microbiota and impairs gut barrier integrity, resulting in increased maternal circulating LPS, which may ultimate contribute to changes in placental vascularization and fetal gut development.
31081119	1	23	theme	intestinal	692:701	arg1	inflammation					703:714	fetal intestinal inflammation	686:714	fetal intestinal inflammation	686:714	KEY POINTS Maternal obesity has been associated with shifts in intestinal microbiota, which may contribute to impaired barrier function Impaired barrier function may expose the placenta and fetus to pro-inflammatory mediators We investigated the impacts of diet-induced obesity in mice on maternal and fetal intestinal structure and placental vascularization Diet-induced obesity decreased maternal intestinal short chain fatty acids and their receptors, impaired gut barrier integrity and was associated with fetal intestinal inflammation.
31081119	1	24	theme	Maternal	187:194	arg1	obesity					196:202	Maternal obesity	187:202	Maternal obesity	187:202	KEY POINTS Maternal obesity has been associated with shifts in intestinal microbiota, which may contribute to impaired barrier function Impaired barrier function may expose the placenta and fetus to pro-inflammatory mediators We investigated the impacts of diet-induced obesity in mice on maternal and fetal intestinal structure and placental vascularization Diet-induced obesity decreased maternal intestinal short chain fatty acids and their receptors, impaired gut barrier integrity and was associated with fetal intestinal inflammation.
31081119	5	25	theme	high-fat	1332:1339	arg1	HFD					1347:1349	HFD 60% kcal from fat, n = 10-14; ad libitum	1347:1390	HFD	1347:1349	In the present study, we generated cohorts of female C57BL/6J mice fed a control (17% kcal fat, n = 10-14) or a high-fat diet (HFD 60% kcal from fat, n = 10-14; ad libitum) aiming to investigate the impact on the maternal gut microbiota, intestinal inflammation and gut barrier integrity, placental inflammation and fetal intestinal development at embryonic day 18.5.
31081119	5	25	theme	high-fat	1332:1339	arg1	diet					1341:1344	a high-fat diet	1330:1344	a high-fat diet (HFD 60% kcal from fat, n = 10-14; ad libitum)	1330:1391	In the present study, we generated cohorts of female C57BL/6J mice fed a control (17% kcal fat, n = 10-14) or a high-fat diet (HFD 60% kcal from fat, n = 10-14; ad libitum) aiming to investigate the impact on the maternal gut microbiota, intestinal inflammation and gut barrier integrity, placental inflammation and fetal intestinal development at embryonic day 18.5.
31081119	2	26	theme	reticulum	875:883	arg1	markers					892:898	endoplasmic reticulum stress markers	863:898	endoplasmic reticulum stress markers	863:898	Placenta from obese mothers showed blood vessel immaturity, hypoxia, increased transcript levels of inflammation, autophagy and altered levels of endoplasmic reticulum stress markers.
31081119	4	27	theme	metabolic	1184:1192	arg1	adaptations					1194:1204	metabolic adaptations	1184:1204	metabolic adaptations to pregnancy	1184:1217	ABSTRACT Shifts in maternal intestinal microbiota have been implicated in metabolic adaptations to pregnancy.
31081119	5	28	theme	C57BL/6J	1273:1280	arg1	mice					1282:1285	female C57BL/6J mice	1266:1285	female C57BL/6J mice fed a control (17% kcal fat, n = 10-14) or a high-fat diet (HFD 60% kcal from fat, n = 10-14; ad libitum) aiming to investigate the impact on the maternal gut microbiota, intestinal inflammation and gut barrier integrity, placental inflammation and fetal intestinal development at embryonic day 18.5	1266:1585	In the present study, we generated cohorts of female C57BL/6J mice fed a control (17% kcal fat, n = 10-14) or a high-fat diet (HFD 60% kcal from fat, n = 10-14; ad libitum) aiming to investigate the impact on the maternal gut microbiota, intestinal inflammation and gut barrier integrity, placental inflammation and fetal intestinal development at embryonic day 18.5.
31081119	10	29	theme	B	2420:2420	arg1	activation					2385:2394	increased activation	2375:2394	increased activation of nuclear factor-kappa B	2375:2420	HFD exposed fetuses had increased activation of nuclear factor-kappa B and inhibition of the unfolded protein response in the developing intestine.
31081119	10	29	theme	B	2420:2420	arg1	inhibition					2426:2435	inhibition	2426:2435	inhibition of the unfolded protein response in the developing intestine	2426:2496	HFD exposed fetuses had increased activation of nuclear factor-kappa B and inhibition of the unfolded protein response in the developing intestine.
31081119	5	30	dep	control	1293:1299	arg1	fat					1311:1313	17% kcal fat	1302:1313	17% kcal fat	1302:1313	In the present study, we generated cohorts of female C57BL/6J mice fed a control (17% kcal fat, n = 10-14) or a high-fat diet (HFD 60% kcal from fat, n = 10-14; ad libitum) aiming to investigate the impact on the maternal gut microbiota, intestinal inflammation and gut barrier integrity, placental inflammation and fetal intestinal development at embryonic day 18.5.
31081119	5	30	dep	control	1293:1299	arg1	n = 10-14					1316:1324	n = 10-14	1316:1324	n = 10-14	1316:1324	In the present study, we generated cohorts of female C57BL/6J mice fed a control (17% kcal fat, n = 10-14) or a high-fat diet (HFD 60% kcal from fat, n = 10-14; ad libitum) aiming to investigate the impact on the maternal gut microbiota, intestinal inflammation and gut barrier integrity, placental inflammation and fetal intestinal development at embryonic day 18.5.
31081119	0	31	theme	gut	135:137	arg1	proteins					147:154	altered fetal gut barrier proteins	121:154	altered fetal gut barrier proteins	121:154	High-fat diet intake modulates maternal intestinal adaptations to pregnancy and results in placental hypoxia, as well as altered fetal gut barrier proteins and immune markers.
31081119	11	32	theme	gut	2646:2648	arg1	integrity					2658:2666	gut barrier integrity	2646:2666	gut barrier integrity	2646:2666	Taken together, these data suggest that HFD intake prior to and during pregnancy shifts the composition of the maternal gut microbiota and impairs gut barrier integrity, resulting in increased maternal circulating LPS, which may ultimate contribute to changes in placental vascularization and fetal gut development.
31081119	11	33	theme	maternal	2610:2617	arg1	microbiota					2623:2632	the maternal gut microbiota	2606:2632	the maternal gut microbiota	2606:2632	Taken together, these data suggest that HFD intake prior to and during pregnancy shifts the composition of the maternal gut microbiota and impairs gut barrier integrity, resulting in increased maternal circulating LPS, which may ultimate contribute to changes in placental vascularization and fetal gut development.
31081119	7	34	theme	mRNA	1777:1780	arg1	levels					1782:1787	decreased maternal intestinal mRNA levels	1747:1787	decreased maternal intestinal mRNA levels of SCFA receptor Gpr41	1747:1810	These diet-induced shifts paralleled decreased maternal intestinal mRNA levels of SCFA receptor Gpr41, modestly decreased cecal butyrate, and altered mRNA levels of inflammatory cytokines and immune cell markers in the maternal intestine.
31081119	10	35	theme	nuclear	2399:2405	arg1	B					2420:2420	nuclear factor-kappa B	2399:2420	nuclear factor-kappa B	2399:2420	HFD exposed fetuses had increased activation of nuclear factor-kappa B and inhibition of the unfolded protein response in the developing intestine.
31081119	8	36	theme	Maternal	1949:1956	arg1	HFD					1958:1960	Maternal HFD	1949:1960	Maternal HFD	1949:1960	Maternal HFD resulted in impaired gut barrier integrity, with corresponding increases in circulating maternal levels of lipopolysaccharide (LPS) and tumour necrosis factor.
31081119	2	37	theme	obese	731:735	arg1	mothers					737:743	obese mothers	731:743	obese mothers	731:743	Placenta from obese mothers showed blood vessel immaturity, hypoxia, increased transcript levels of inflammation, autophagy and altered levels of endoplasmic reticulum stress markers.
31081119	8	38	theme	impaired	1974:1981	arg1	integrity					1995:2003	impaired gut barrier integrity	1974:2003	impaired gut barrier integrity	1974:2003	Maternal HFD resulted in impaired gut barrier integrity, with corresponding increases in circulating maternal levels of lipopolysaccharide (LPS) and tumour necrosis factor.
31081119	7	39	theme	maternal	1757:1764	arg1	levels					1782:1787	decreased maternal intestinal mRNA levels	1747:1787	decreased maternal intestinal mRNA levels of SCFA receptor Gpr41	1747:1810	These diet-induced shifts paralleled decreased maternal intestinal mRNA levels of SCFA receptor Gpr41, modestly decreased cecal butyrate, and altered mRNA levels of inflammatory cytokines and immune cell markers in the maternal intestine.
31081119	10	40	from	response	2461:2468	arg1	intestine					2488:2496	the developing intestine	2473:2496	the developing intestine	2473:2496	HFD exposed fetuses had increased activation of nuclear factor-kappa B and inhibition of the unfolded protein response in the developing intestine.
31081119	11	41	theme	placental	2762:2770	arg1	vascularization					2772:2786	placental vascularization	2762:2786	placental vascularization	2762:2786	Taken together, these data suggest that HFD intake prior to and during pregnancy shifts the composition of the maternal gut microbiota and impairs gut barrier integrity, resulting in increased maternal circulating LPS, which may ultimate contribute to changes in placental vascularization and fetal gut development.
31081119	1	42	theme	gut	640:642	arg1	integrity					652:660	gut barrier integrity	640:660	gut barrier integrity	640:660	KEY POINTS Maternal obesity has been associated with shifts in intestinal microbiota, which may contribute to impaired barrier function Impaired barrier function may expose the placenta and fetus to pro-inflammatory mediators We investigated the impacts of diet-induced obesity in mice on maternal and fetal intestinal structure and placental vascularization Diet-induced obesity decreased maternal intestinal short chain fatty acids and their receptors, impaired gut barrier integrity and was associated with fetal intestinal inflammation.
31081119	9	43	theme	mRNA	2288:2291	arg1	levels					2293:2298	altered mRNA levels	2280:2298	altered mRNA levels of inflammation, autophagy, and ER stress markers	2280:2348	Placentas from HFD dams demonstrated blood vessel immaturity and hypoxia; decreased free carnitine, acylcarnitine derivatives and trimethylamine-N-oxide; and altered mRNA levels of inflammation, autophagy, and ER stress markers.
31081119	8	44	theme	barrier	1987:1993	arg1	integrity					1995:2003	impaired gut barrier integrity	1974:2003	impaired gut barrier integrity	1974:2003	Maternal HFD resulted in impaired gut barrier integrity, with corresponding increases in circulating maternal levels of lipopolysaccharide (LPS) and tumour necrosis factor.
31081119	1	45	theme	barrier	321:327	arg1	function					329:336	Impaired barrier function	312:336	Impaired barrier function	312:336	KEY POINTS Maternal obesity has been associated with shifts in intestinal microbiota, which may contribute to impaired barrier function Impaired barrier function may expose the placenta and fetus to pro-inflammatory mediators We investigated the impacts of diet-induced obesity in mice on maternal and fetal intestinal structure and placental vascularization Diet-induced obesity decreased maternal intestinal short chain fatty acids and their receptors, impaired gut barrier integrity and was associated with fetal intestinal inflammation.
31081119	3	46	theme	intestinal	934:943	arg1	changes					945:951	maternal intestinal changes	925:951	maternal intestinal changes	925:951	These data suggest that maternal intestinal changes probably contribute to adverse placental adaptations and also impart an increased risk of obesity in the offspring via alterations in fetal gut development.
31081119	11	47	theme	HFD	2539:2541	arg1	intake					2543:2548	HFD intake	2539:2548	HFD intake prior to and during pregnancy	2539:2578	Taken together, these data suggest that HFD intake prior to and during pregnancy shifts the composition of the maternal gut microbiota and impairs gut barrier integrity, resulting in increased maternal circulating LPS, which may ultimate contribute to changes in placental vascularization and fetal gut development.
31081119	2	48	theme	vessel	758:763	arg1	immaturity					765:774	blood vessel immaturity	752:774	blood vessel immaturity	752:774	Placenta from obese mothers showed blood vessel immaturity, hypoxia, increased transcript levels of inflammation, autophagy and altered levels of endoplasmic reticulum stress markers.
31081119	2	48	theme	vessel	758:763	arg1	levels					807:812	increased transcript levels	786:812	increased transcript levels of inflammation, autophagy and altered levels of endoplasmic reticulum stress markers	786:898	Placenta from obese mothers showed blood vessel immaturity, hypoxia, increased transcript levels of inflammation, autophagy and altered levels of endoplasmic reticulum stress markers.
31081119	2	48	theme	vessel	758:763	arg1	hypoxia					777:783	hypoxia	777:783	hypoxia	777:783	Placenta from obese mothers showed blood vessel immaturity, hypoxia, increased transcript levels of inflammation, autophagy and altered levels of endoplasmic reticulum stress markers.
31081119	5	49	theme	 kcal	1305:1309	arg1	fat					1311:1313	17% kcal fat	1302:1313	17% kcal fat	1302:1313	In the present study, we generated cohorts of female C57BL/6J mice fed a control (17% kcal fat, n = 10-14) or a high-fat diet (HFD 60% kcal from fat, n = 10-14; ad libitum) aiming to investigate the impact on the maternal gut microbiota, intestinal inflammation and gut barrier integrity, placental inflammation and fetal intestinal development at embryonic day 18.5.
31081119	5	49	theme	 kcal	1305:1309	arg1	n = 10-14					1316:1324	n = 10-14	1316:1324	n = 10-14	1316:1324	In the present study, we generated cohorts of female C57BL/6J mice fed a control (17% kcal fat, n = 10-14) or a high-fat diet (HFD 60% kcal from fat, n = 10-14; ad libitum) aiming to investigate the impact on the maternal gut microbiota, intestinal inflammation and gut barrier integrity, placental inflammation and fetal intestinal development at embryonic day 18.5.
31081119	7	50	theme	diet-induced	1716:1727	arg1	shifts					1729:1734	These diet-induced shifts	1710:1734	These diet-induced shifts	1710:1734	These diet-induced shifts paralleled decreased maternal intestinal mRNA levels of SCFA receptor Gpr41, modestly decreased cecal butyrate, and altered mRNA levels of inflammatory cytokines and immune cell markers in the maternal intestine.
31081119	10	51	from	intestine	2488:2496	arg1	activation					2385:2394	increased activation	2375:2394	increased activation of nuclear factor-kappa B	2375:2420	HFD exposed fetuses had increased activation of nuclear factor-kappa B and inhibition of the unfolded protein response in the developing intestine.
31081119	10	51	from	intestine	2488:2496	arg1	inhibition					2426:2435	inhibition	2426:2435	inhibition of the unfolded protein response in the developing intestine	2426:2496	HFD exposed fetuses had increased activation of nuclear factor-kappa B and inhibition of the unfolded protein response in the developing intestine.
31081119	5	52	theme	barrier	1490:1496	arg1	integrity					1498:1506	gut barrier integrity	1486:1506	gut barrier integrity	1486:1506	In the present study, we generated cohorts of female C57BL/6J mice fed a control (17% kcal fat, n = 10-14) or a high-fat diet (HFD 60% kcal from fat, n = 10-14; ad libitum) aiming to investigate the impact on the maternal gut microbiota, intestinal inflammation and gut barrier integrity, placental inflammation and fetal intestinal development at embryonic day 18.5.
31081119	0	53	theme	placental	91:99	arg1	hypoxia					101:107	placental hypoxia	91:107	placental hypoxia	91:107	High-fat diet intake modulates maternal intestinal adaptations to pregnancy and results in placental hypoxia, as well as altered fetal gut barrier proteins and immune markers.
31081119	6	54	theme	fatty	1657:1661	arg1	SCFA					1669:1672	SCFA	1669:1672	SCFA	1669:1672	HFD was associated with decreased relative abundances of short-chain fatty acid (SCFA) producing genera during pregnancy.
31081119	6	54	theme	fatty	1657:1661	arg1	acid					1663:1666	short-chain fatty acid	1645:1666	short-chain fatty acid (SCFA) producing genera during pregnancy	1645:1707	HFD was associated with decreased relative abundances of short-chain fatty acid (SCFA) producing genera during pregnancy.
31081119	10	55	theme	protein	2453:2459	arg1	response					2461:2468	the unfolded protein response	2440:2468	the unfolded protein response in the developing intestine	2440:2496	HFD exposed fetuses had increased activation of nuclear factor-kappa B and inhibition of the unfolded protein response in the developing intestine.
31081119	1	56	theme	intestinal	575:584	arg1	acids					604:608	maternal intestinal short chain fatty acids	566:608	maternal intestinal short chain fatty acids	566:608	KEY POINTS Maternal obesity has been associated with shifts in intestinal microbiota, which may contribute to impaired barrier function Impaired barrier function may expose the placenta and fetus to pro-inflammatory mediators We investigated the impacts of diet-induced obesity in mice on maternal and fetal intestinal structure and placental vascularization Diet-induced obesity decreased maternal intestinal short chain fatty acids and their receptors, impaired gut barrier integrity and was associated with fetal intestinal inflammation.
31081119	8	57	theme	tumour	2098:2103	arg1	factor					2114:2119	tumour necrosis factor	2098:2119	tumour necrosis factor	2098:2119	Maternal HFD resulted in impaired gut barrier integrity, with corresponding increases in circulating maternal levels of lipopolysaccharide (LPS) and tumour necrosis factor.
31081119	11	58	theme	prior	2550:2554	arg1	intake					2543:2548	HFD intake	2539:2548	HFD intake prior to and during pregnancy	2539:2578	Taken together, these data suggest that HFD intake prior to and during pregnancy shifts the composition of the maternal gut microbiota and impairs gut barrier integrity, resulting in increased maternal circulating LPS, which may ultimate contribute to changes in placental vascularization and fetal gut development.
31081119	5	59	theme	intestinal	1458:1467	arg1	inflammation					1469:1480	intestinal inflammation	1458:1480	intestinal inflammation	1458:1480	In the present study, we generated cohorts of female C57BL/6J mice fed a control (17% kcal fat, n = 10-14) or a high-fat diet (HFD 60% kcal from fat, n = 10-14; ad libitum) aiming to investigate the impact on the maternal gut microbiota, intestinal inflammation and gut barrier integrity, placental inflammation and fetal intestinal development at embryonic day 18.5.
31081119	1	60	theme	chain	592:596	arg1	acids					604:608	maternal intestinal short chain fatty acids	566:608	maternal intestinal short chain fatty acids	566:608	KEY POINTS Maternal obesity has been associated with shifts in intestinal microbiota, which may contribute to impaired barrier function Impaired barrier function may expose the placenta and fetus to pro-inflammatory mediators We investigated the impacts of diet-induced obesity in mice on maternal and fetal intestinal structure and placental vascularization Diet-induced obesity decreased maternal intestinal short chain fatty acids and their receptors, impaired gut barrier integrity and was associated with fetal intestinal inflammation.
31081119	9	61	theme	ER	2332:2333	arg1	stress					2335:2340	ER stress	2332:2340	ER stress	2332:2340	Placentas from HFD dams demonstrated blood vessel immaturity and hypoxia; decreased free carnitine, acylcarnitine derivatives and trimethylamine-N-oxide; and altered mRNA levels of inflammation, autophagy, and ER stress markers.
31081119	0	62	theme	High-fat	0:7	arg1	intake					14:19	High-fat diet intake	0:19	High-fat diet intake	0:19	High-fat diet intake modulates maternal intestinal adaptations to pregnancy and results in placental hypoxia, as well as altered fetal gut barrier proteins and immune markers.
31081119	6	63	theme	relative	1622:1629	arg1	abundances					1631:1640	decreased relative abundances	1612:1640	decreased relative abundances of short-chain fatty acid (SCFA) producing genera during pregnancy	1612:1707	HFD was associated with decreased relative abundances of short-chain fatty acid (SCFA) producing genera during pregnancy.
31081119	8	64	theme	factor	2114:2119	arg1	levels					2059:2064	circulating maternal levels	2038:2064	circulating maternal levels of lipopolysaccharide (LPS) and tumour necrosis factor	2038:2119	Maternal HFD resulted in impaired gut barrier integrity, with corresponding increases in circulating maternal levels of lipopolysaccharide (LPS) and tumour necrosis factor.
31081119	10	65	theme	developing	2477:2486	arg1	intestine					2488:2496	the developing intestine	2473:2496	the developing intestine	2473:2496	HFD exposed fetuses had increased activation of nuclear factor-kappa B and inhibition of the unfolded protein response in the developing intestine.
31081119	11	66	theme	maternal	2692:2699	arg1	LPS					2713:2715	increased maternal circulating LPS	2682:2715	increased maternal circulating LPS	2682:2715	Taken together, these data suggest that HFD intake prior to and during pregnancy shifts the composition of the maternal gut microbiota and impairs gut barrier integrity, resulting in increased maternal circulating LPS, which may ultimate contribute to changes in placental vascularization and fetal gut development.
31081119	5	67	theme	fetal	1536:1540	arg1	development					1553:1563	fetal intestinal development	1536:1563	fetal intestinal development	1536:1563	In the present study, we generated cohorts of female C57BL/6J mice fed a control (17% kcal fat, n = 10-14) or a high-fat diet (HFD 60% kcal from fat, n = 10-14; ad libitum) aiming to investigate the impact on the maternal gut microbiota, intestinal inflammation and gut barrier integrity, placental inflammation and fetal intestinal development at embryonic day 18.5.
31081119	7	68	theme	maternal	1929:1936	arg1	intestine					1938:1946	the maternal intestine	1925:1946	the maternal intestine	1925:1946	These diet-induced shifts paralleled decreased maternal intestinal mRNA levels of SCFA receptor Gpr41, modestly decreased cecal butyrate, and altered mRNA levels of inflammatory cytokines and immune cell markers in the maternal intestine.
31081119	5	69	from	18.5	1582:1585	arg1	impact					1419:1424	the impact	1415:1424	the impact on the maternal gut microbiota, intestinal inflammation and gut barrier integrity, placental inflammation and fetal intestinal development at embryonic day 18.5	1415:1585	In the present study, we generated cohorts of female C57BL/6J mice fed a control (17% kcal fat, n = 10-14) or a high-fat diet (HFD 60% kcal from fat, n = 10-14; ad libitum) aiming to investigate the impact on the maternal gut microbiota, intestinal inflammation and gut barrier integrity, placental inflammation and fetal intestinal development at embryonic day 18.5.
31081119	3	70	theme	placental	984:992	arg1	adaptations					994:1004	adverse placental adaptations	976:1004	adverse placental adaptations	976:1004	These data suggest that maternal intestinal changes probably contribute to adverse placental adaptations and also impart an increased risk of obesity in the offspring via alterations in fetal gut development.
31081119	7	71	from	levels	1865:1870	arg1	intestine					1938:1946	the maternal intestine	1925:1946	the maternal intestine	1925:1946	These diet-induced shifts paralleled decreased maternal intestinal mRNA levels of SCFA receptor Gpr41, modestly decreased cecal butyrate, and altered mRNA levels of inflammatory cytokines and immune cell markers in the maternal intestine.
31081119	7	72	theme	intestinal	1766:1775	arg1	levels					1782:1787	decreased maternal intestinal mRNA levels	1747:1787	decreased maternal intestinal mRNA levels of SCFA receptor Gpr41	1747:1810	These diet-induced shifts paralleled decreased maternal intestinal mRNA levels of SCFA receptor Gpr41, modestly decreased cecal butyrate, and altered mRNA levels of inflammatory cytokines and immune cell markers in the maternal intestine.
31081119	0	73	theme	maternal	31:38	arg1	adaptations					51:61	maternal intestinal adaptations	31:61	maternal intestinal adaptations to pregnancy	31:74	High-fat diet intake modulates maternal intestinal adaptations to pregnancy and results in placental hypoxia, as well as altered fetal gut barrier proteins and immune markers.
31081119	1	74	theme	impaired	286:293	arg1	function					303:310	impaired barrier function	286:310	impaired barrier function	286:310	KEY POINTS Maternal obesity has been associated with shifts in intestinal microbiota, which may contribute to impaired barrier function Impaired barrier function may expose the placenta and fetus to pro-inflammatory mediators We investigated the impacts of diet-induced obesity in mice on maternal and fetal intestinal structure and placental vascularization Diet-induced obesity decreased maternal intestinal short chain fatty acids and their receptors, impaired gut barrier integrity and was associated with fetal intestinal inflammation.
31081119	3	75	theme	increased	1025:1033	arg1	risk					1035:1038	an increased risk	1022:1038	an increased risk of obesity in the offspring	1022:1066	These data suggest that maternal intestinal changes probably contribute to adverse placental adaptations and also impart an increased risk of obesity in the offspring via alterations in fetal gut development.
31081119	3	76	from	risk	1035:1038	arg1	offspring					1058:1066	the offspring	1054:1066	the offspring	1054:1066	These data suggest that maternal intestinal changes probably contribute to adverse placental adaptations and also impart an increased risk of obesity in the offspring via alterations in fetal gut development.
31081119	9	77	from	dams	2141:2144	arg1	Placentas					2122:2130	Placentas	2122:2130	Placentas from HFD dams	2122:2144	Placentas from HFD dams demonstrated blood vessel immaturity and hypoxia; decreased free carnitine, acylcarnitine derivatives and trimethylamine-N-oxide; and altered mRNA levels of inflammation, autophagy, and ER stress markers.
31081119	7	78	theme	cell	1909:1912	arg1	markers					1914:1920	immune cell markers	1902:1920	immune cell markers in the maternal intestine	1902:1946	These diet-induced shifts paralleled decreased maternal intestinal mRNA levels of SCFA receptor Gpr41, modestly decreased cecal butyrate, and altered mRNA levels of inflammatory cytokines and immune cell markers in the maternal intestine.
31081119	7	79	theme	mRNA	1860:1863	arg1	levels					1865:1870	altered mRNA levels	1852:1870	altered mRNA levels of inflammatory cytokines and immune cell markers in the maternal intestine	1852:1946	These diet-induced shifts paralleled decreased maternal intestinal mRNA levels of SCFA receptor Gpr41, modestly decreased cecal butyrate, and altered mRNA levels of inflammatory cytokines and immune cell markers in the maternal intestine.
31081119	5	80	from	impact	1419:1424	arg1	inflammation					1519:1530	placental inflammation	1509:1530	placental inflammation	1509:1530	In the present study, we generated cohorts of female C57BL/6J mice fed a control (17% kcal fat, n = 10-14) or a high-fat diet (HFD 60% kcal from fat, n = 10-14; ad libitum) aiming to investigate the impact on the maternal gut microbiota, intestinal inflammation and gut barrier integrity, placental inflammation and fetal intestinal development at embryonic day 18.5.
31081119	5	80	from	impact	1419:1424	arg1	microbiota					1446:1455	the maternal gut microbiota	1429:1455	the maternal gut microbiota	1429:1455	In the present study, we generated cohorts of female C57BL/6J mice fed a control (17% kcal fat, n = 10-14) or a high-fat diet (HFD 60% kcal from fat, n = 10-14; ad libitum) aiming to investigate the impact on the maternal gut microbiota, intestinal inflammation and gut barrier integrity, placental inflammation and fetal intestinal development at embryonic day 18.5.
31081119	5	80	from	impact	1419:1424	arg1	inflammation					1469:1480	intestinal inflammation	1458:1480	intestinal inflammation	1458:1480	In the present study, we generated cohorts of female C57BL/6J mice fed a control (17% kcal fat, n = 10-14) or a high-fat diet (HFD 60% kcal from fat, n = 10-14; ad libitum) aiming to investigate the impact on the maternal gut microbiota, intestinal inflammation and gut barrier integrity, placental inflammation and fetal intestinal development at embryonic day 18.5.
31081119	5	80	from	impact	1419:1424	arg1	development					1553:1563	fetal intestinal development	1536:1563	fetal intestinal development	1536:1563	In the present study, we generated cohorts of female C57BL/6J mice fed a control (17% kcal fat, n = 10-14) or a high-fat diet (HFD 60% kcal from fat, n = 10-14; ad libitum) aiming to investigate the impact on the maternal gut microbiota, intestinal inflammation and gut barrier integrity, placental inflammation and fetal intestinal development at embryonic day 18.5.
31081119	1	81	dep	placenta	353:360	arg1	the					349:351	the	349:351	the	349:351	KEY POINTS Maternal obesity has been associated with shifts in intestinal microbiota, which may contribute to impaired barrier function Impaired barrier function may expose the placenta and fetus to pro-inflammatory mediators We investigated the impacts of diet-induced obesity in mice on maternal and fetal intestinal structure and placental vascularization Diet-induced obesity decreased maternal intestinal short chain fatty acids and their receptors, impaired gut barrier integrity and was associated with fetal intestinal inflammation.
31081119	1	82	theme	placental	509:517	arg1	vascularization					519:533	placental vascularization	509:533	placental vascularization	509:533	KEY POINTS Maternal obesity has been associated with shifts in intestinal microbiota, which may contribute to impaired barrier function Impaired barrier function may expose the placenta and fetus to pro-inflammatory mediators We investigated the impacts of diet-induced obesity in mice on maternal and fetal intestinal structure and placental vascularization Diet-induced obesity decreased maternal intestinal short chain fatty acids and their receptors, impaired gut barrier integrity and was associated with fetal intestinal inflammation.
31081119	1	83	theme	intestinal	484:493	arg1	structure					495:503	intestinal structure	484:503	intestinal structure	484:503	KEY POINTS Maternal obesity has been associated with shifts in intestinal microbiota, which may contribute to impaired barrier function Impaired barrier function may expose the placenta and fetus to pro-inflammatory mediators We investigated the impacts of diet-induced obesity in mice on maternal and fetal intestinal structure and placental vascularization Diet-induced obesity decreased maternal intestinal short chain fatty acids and their receptors, impaired gut barrier integrity and was associated with fetal intestinal inflammation.
31081119	2	84	theme	altered	845:851	arg1	levels					853:858	altered levels	845:858	altered levels of endoplasmic reticulum stress markers	845:898	Placenta from obese mothers showed blood vessel immaturity, hypoxia, increased transcript levels of inflammation, autophagy and altered levels of endoplasmic reticulum stress markers.
31081119	1	85	dep	POINTS	180:185	arg1	associated					213:222	associated	213:222	has been associated with shifts in intestinal microbiota, which may contribute to impaired barrier function Impaired barrier function may expose the placenta and fetus to pro-inflammatory mediators We investigated the impacts of diet-induced obesity in mice on maternal and fetal intestinal structure and placental vascularization Diet-induced obesity decreased maternal intestinal short chain fatty acids and their receptors, impaired gut barrier integrity and was associated with fetal intestinal inflammation	204:714	KEY POINTS Maternal obesity has been associated with shifts in intestinal microbiota, which may contribute to impaired barrier function Impaired barrier function may expose the placenta and fetus to pro-inflammatory mediators We investigated the impacts of diet-induced obesity in mice on maternal and fetal intestinal structure and placental vascularization Diet-induced obesity decreased maternal intestinal short chain fatty acids and their receptors, impaired gut barrier integrity and was associated with fetal intestinal inflammation.
31081119	1	86	theme	Diet-induced	535:546	arg1	obesity					548:554	Diet-induced obesity	535:554	Diet-induced obesity	535:554	KEY POINTS Maternal obesity has been associated with shifts in intestinal microbiota, which may contribute to impaired barrier function Impaired barrier function may expose the placenta and fetus to pro-inflammatory mediators We investigated the impacts of diet-induced obesity in mice on maternal and fetal intestinal structure and placental vascularization Diet-induced obesity decreased maternal intestinal short chain fatty acids and their receptors, impaired gut barrier integrity and was associated with fetal intestinal inflammation.
31081119	1	87	from	mice	457:460	arg1	impacts					422:428	the impacts	418:428	the impacts of diet-induced obesity in mice on maternal and fetal intestinal structure and placental vascularization Diet-induced obesity	418:554	KEY POINTS Maternal obesity has been associated with shifts in intestinal microbiota, which may contribute to impaired barrier function Impaired barrier function may expose the placenta and fetus to pro-inflammatory mediators We investigated the impacts of diet-induced obesity in mice on maternal and fetal intestinal structure and placental vascularization Diet-induced obesity decreased maternal intestinal short chain fatty acids and their receptors, impaired gut barrier integrity and was associated with fetal intestinal inflammation.
31081119	2	88	theme	markers	892:898	arg1	levels					853:858	altered levels	845:858	altered levels of endoplasmic reticulum stress markers	845:898	Placenta from obese mothers showed blood vessel immaturity, hypoxia, increased transcript levels of inflammation, autophagy and altered levels of endoplasmic reticulum stress markers.
31081119	2	88	theme	markers	892:898	arg1	inflammation					817:828	inflammation	817:828	inflammation	817:828	Placenta from obese mothers showed blood vessel immaturity, hypoxia, increased transcript levels of inflammation, autophagy and altered levels of endoplasmic reticulum stress markers.
31081119	2	88	theme	markers	892:898	arg1	autophagy					831:839	autophagy	831:839	autophagy	831:839	Placenta from obese mothers showed blood vessel immaturity, hypoxia, increased transcript levels of inflammation, autophagy and altered levels of endoplasmic reticulum stress markers.
31081119	5	89	theme	present	1227:1233	arg1	study					1235:1239	the present study	1223:1239	the present study	1223:1239	In the present study, we generated cohorts of female C57BL/6J mice fed a control (17% kcal fat, n = 10-14) or a high-fat diet (HFD 60% kcal from fat, n = 10-14; ad libitum) aiming to investigate the impact on the maternal gut microbiota, intestinal inflammation and gut barrier integrity, placental inflammation and fetal intestinal development at embryonic day 18.5.
31081119	9	90	theme	blood	2159:2163	arg1	immaturity					2172:2181	blood vessel immaturity	2159:2181	blood vessel immaturity	2159:2181	Placentas from HFD dams demonstrated blood vessel immaturity and hypoxia; decreased free carnitine, acylcarnitine derivatives and trimethylamine-N-oxide; and altered mRNA levels of inflammation, autophagy, and ER stress markers.
31081119	5	91	theme	female	1266:1271	arg1	mice					1282:1285	female C57BL/6J mice	1266:1285	female C57BL/6J mice fed a control (17% kcal fat, n = 10-14) or a high-fat diet (HFD 60% kcal from fat, n = 10-14; ad libitum) aiming to investigate the impact on the maternal gut microbiota, intestinal inflammation and gut barrier integrity, placental inflammation and fetal intestinal development at embryonic day 18.5	1266:1585	In the present study, we generated cohorts of female C57BL/6J mice fed a control (17% kcal fat, n = 10-14) or a high-fat diet (HFD 60% kcal from fat, n = 10-14; ad libitum) aiming to investigate the impact on the maternal gut microbiota, intestinal inflammation and gut barrier integrity, placental inflammation and fetal intestinal development at embryonic day 18.5.
31081119	7	92	theme	decreased	1822:1830	arg1	butyrate					1838:1845	modestly decreased cecal butyrate	1813:1845	modestly decreased cecal butyrate	1813:1845	These diet-induced shifts paralleled decreased maternal intestinal mRNA levels of SCFA receptor Gpr41, modestly decreased cecal butyrate, and altered mRNA levels of inflammatory cytokines and immune cell markers in the maternal intestine.
31081119	1	93	from	obesity	446:452	arg1	mice					457:460	mice	457:460	mice	457:460	KEY POINTS Maternal obesity has been associated with shifts in intestinal microbiota, which may contribute to impaired barrier function Impaired barrier function may expose the placenta and fetus to pro-inflammatory mediators We investigated the impacts of diet-induced obesity in mice on maternal and fetal intestinal structure and placental vascularization Diet-induced obesity decreased maternal intestinal short chain fatty acids and their receptors, impaired gut barrier integrity and was associated with fetal intestinal inflammation.
31081119	4	94	theme	maternal	1129:1136	arg1	microbiota					1149:1158	maternal intestinal microbiota	1129:1158	maternal intestinal microbiota	1129:1158	ABSTRACT Shifts in maternal intestinal microbiota have been implicated in metabolic adaptations to pregnancy.
31081119	5	95	theme	embryonic	1568:1576	arg1	18.5					1582:1585	embryonic day 18.5	1568:1585	embryonic day 18.5	1568:1585	In the present study, we generated cohorts of female C57BL/6J mice fed a control (17% kcal fat, n = 10-14) or a high-fat diet (HFD 60% kcal from fat, n = 10-14; ad libitum) aiming to investigate the impact on the maternal gut microbiota, intestinal inflammation and gut barrier integrity, placental inflammation and fetal intestinal development at embryonic day 18.5.
31081119	1	96	theme	obesity	446:452	arg1	impacts					422:428	the impacts	418:428	the impacts of diet-induced obesity in mice on maternal and fetal intestinal structure and placental vascularization Diet-induced obesity	418:554	KEY POINTS Maternal obesity has been associated with shifts in intestinal microbiota, which may contribute to impaired barrier function Impaired barrier function may expose the placenta and fetus to pro-inflammatory mediators We investigated the impacts of diet-induced obesity in mice on maternal and fetal intestinal structure and placental vascularization Diet-induced obesity decreased maternal intestinal short chain fatty acids and their receptors, impaired gut barrier integrity and was associated with fetal intestinal inflammation.
31081119	5	97	theme	mice	1282:1285	arg1	cohorts					1255:1261	cohorts	1255:1261	cohorts of female C57BL/6J mice fed a control (17% kcal fat, n = 10-14) or a high-fat diet (HFD 60% kcal from fat, n = 10-14; ad libitum) aiming to investigate the impact on the maternal gut microbiota, intestinal inflammation and gut barrier integrity, placental inflammation and fetal intestinal development at embryonic day 18.5	1255:1585	In the present study, we generated cohorts of female C57BL/6J mice fed a control (17% kcal fat, n = 10-14) or a high-fat diet (HFD 60% kcal from fat, n = 10-14; ad libitum) aiming to investigate the impact on the maternal gut microbiota, intestinal inflammation and gut barrier integrity, placental inflammation and fetal intestinal development at embryonic day 18.5.
31081119	11	98	theme	barrier	2650:2656	arg1	integrity					2658:2666	gut barrier integrity	2646:2666	gut barrier integrity	2646:2666	Taken together, these data suggest that HFD intake prior to and during pregnancy shifts the composition of the maternal gut microbiota and impairs gut barrier integrity, resulting in increased maternal circulating LPS, which may ultimate contribute to changes in placental vascularization and fetal gut development.
31081119	5	99	theme	gut	1442:1444	arg1	microbiota					1446:1455	the maternal gut microbiota	1429:1455	the maternal gut microbiota	1429:1455	In the present study, we generated cohorts of female C57BL/6J mice fed a control (17% kcal fat, n = 10-14) or a high-fat diet (HFD 60% kcal from fat, n = 10-14; ad libitum) aiming to investigate the impact on the maternal gut microbiota, intestinal inflammation and gut barrier integrity, placental inflammation and fetal intestinal development at embryonic day 18.5.
31081119	10	100	theme	HFD	2351:2353	arg1	fetuses					2363:2369	HFD exposed fetuses	2351:2369	HFD exposed fetuses	2351:2369	HFD exposed fetuses had increased activation of nuclear factor-kappa B and inhibition of the unfolded protein response in the developing intestine.
31081119	7	101	theme	receptor	1797:1804	arg1	Gpr41					1806:1810	SCFA receptor Gpr41	1792:1810	SCFA receptor Gpr41	1792:1810	These diet-induced shifts paralleled decreased maternal intestinal mRNA levels of SCFA receptor Gpr41, modestly decreased cecal butyrate, and altered mRNA levels of inflammatory cytokines and immune cell markers in the maternal intestine.
31081119	2	102	theme	increased	786:794	arg1	immaturity					765:774	blood vessel immaturity	752:774	blood vessel immaturity	752:774	Placenta from obese mothers showed blood vessel immaturity, hypoxia, increased transcript levels of inflammation, autophagy and altered levels of endoplasmic reticulum stress markers.
31081119	2	102	theme	increased	786:794	arg1	levels					807:812	increased transcript levels	786:812	increased transcript levels of inflammation, autophagy and altered levels of endoplasmic reticulum stress markers	786:898	Placenta from obese mothers showed blood vessel immaturity, hypoxia, increased transcript levels of inflammation, autophagy and altered levels of endoplasmic reticulum stress markers.
31081119	9	103	theme	decreased	2196:2204	arg1	carnitine					2211:2219	decreased free carnitine	2196:2219	decreased free carnitine	2196:2219	Placentas from HFD dams demonstrated blood vessel immaturity and hypoxia; decreased free carnitine, acylcarnitine derivatives and trimethylamine-N-oxide; and altered mRNA levels of inflammation, autophagy, and ER stress markers.
31081119	1	104	theme	maternal	465:472	arg1	structure					495:503	intestinal structure	484:503	intestinal structure	484:503	KEY POINTS Maternal obesity has been associated with shifts in intestinal microbiota, which may contribute to impaired barrier function Impaired barrier function may expose the placenta and fetus to pro-inflammatory mediators We investigated the impacts of diet-induced obesity in mice on maternal and fetal intestinal structure and placental vascularization Diet-induced obesity decreased maternal intestinal short chain fatty acids and their receptors, impaired gut barrier integrity and was associated with fetal intestinal inflammation.
31081119	7	105	from	intestine	1938:1946	arg1	levels					1865:1870	altered mRNA levels	1852:1870	altered mRNA levels of inflammatory cytokines and immune cell markers in the maternal intestine	1852:1946	These diet-induced shifts paralleled decreased maternal intestinal mRNA levels of SCFA receptor Gpr41, modestly decreased cecal butyrate, and altered mRNA levels of inflammatory cytokines and immune cell markers in the maternal intestine.
31081119	7	105	from	intestine	1938:1946	arg1	butyrate					1838:1845	modestly decreased cecal butyrate	1813:1845	modestly decreased cecal butyrate	1813:1845	These diet-induced shifts paralleled decreased maternal intestinal mRNA levels of SCFA receptor Gpr41, modestly decreased cecal butyrate, and altered mRNA levels of inflammatory cytokines and immune cell markers in the maternal intestine.
31081119	7	105	from	intestine	1938:1946	arg1	levels					1782:1787	decreased maternal intestinal mRNA levels	1747:1787	decreased maternal intestinal mRNA levels of SCFA receptor Gpr41	1747:1810	These diet-induced shifts paralleled decreased maternal intestinal mRNA levels of SCFA receptor Gpr41, modestly decreased cecal butyrate, and altered mRNA levels of inflammatory cytokines and immune cell markers in the maternal intestine.
31081119	3	106	theme	fetal	1087:1091	arg1	development					1097:1107	fetal gut development	1087:1107	fetal gut development	1087:1107	These data suggest that maternal intestinal changes probably contribute to adverse placental adaptations and also impart an increased risk of obesity in the offspring via alterations in fetal gut development.
31081119	2	107	theme	autophagy	831:839	arg1	immaturity					765:774	blood vessel immaturity	752:774	blood vessel immaturity	752:774	Placenta from obese mothers showed blood vessel immaturity, hypoxia, increased transcript levels of inflammation, autophagy and altered levels of endoplasmic reticulum stress markers.
31081119	2	107	theme	autophagy	831:839	arg1	levels					807:812	increased transcript levels	786:812	increased transcript levels of inflammation, autophagy and altered levels of endoplasmic reticulum stress markers	786:898	Placenta from obese mothers showed blood vessel immaturity, hypoxia, increased transcript levels of inflammation, autophagy and altered levels of endoplasmic reticulum stress markers.
31081119	11	108	theme	fetal	2792:2796	arg1	development					2802:2812	fetal gut development	2792:2812	fetal gut development	2792:2812	Taken together, these data suggest that HFD intake prior to and during pregnancy shifts the composition of the maternal gut microbiota and impairs gut barrier integrity, resulting in increased maternal circulating LPS, which may ultimate contribute to changes in placental vascularization and fetal gut development.
31081119	1	109	theme	fetal	686:690	arg1	inflammation					703:714	fetal intestinal inflammation	686:714	fetal intestinal inflammation	686:714	KEY POINTS Maternal obesity has been associated with shifts in intestinal microbiota, which may contribute to impaired barrier function Impaired barrier function may expose the placenta and fetus to pro-inflammatory mediators We investigated the impacts of diet-induced obesity in mice on maternal and fetal intestinal structure and placental vascularization Diet-induced obesity decreased maternal intestinal short chain fatty acids and their receptors, impaired gut barrier integrity and was associated with fetal intestinal inflammation.
31081119	1	110	theme	fetal	478:482	arg1	structure					495:503	intestinal structure	484:503	intestinal structure	484:503	KEY POINTS Maternal obesity has been associated with shifts in intestinal microbiota, which may contribute to impaired barrier function Impaired barrier function may expose the placenta and fetus to pro-inflammatory mediators We investigated the impacts of diet-induced obesity in mice on maternal and fetal intestinal structure and placental vascularization Diet-induced obesity decreased maternal intestinal short chain fatty acids and their receptors, impaired gut barrier integrity and was associated with fetal intestinal inflammation.
31081119	10	111	theme	increased	2375:2383	arg1	activation					2385:2394	increased activation	2375:2394	increased activation of nuclear factor-kappa B	2375:2420	HFD exposed fetuses had increased activation of nuclear factor-kappa B and inhibition of the unfolded protein response in the developing intestine.
31081119	10	112	from	inhibition	2426:2435	arg1	intestine					2488:2496	the developing intestine	2473:2496	the developing intestine	2473:2496	HFD exposed fetuses had increased activation of nuclear factor-kappa B and inhibition of the unfolded protein response in the developing intestine.
31081119	7	113	from	levels	1782:1787	arg1	intestine					1938:1946	the maternal intestine	1925:1946	the maternal intestine	1925:1946	These diet-induced shifts paralleled decreased maternal intestinal mRNA levels of SCFA receptor Gpr41, modestly decreased cecal butyrate, and altered mRNA levels of inflammatory cytokines and immune cell markers in the maternal intestine.
31081119	2	114	theme	inflammation	817:828	arg1	immaturity					765:774	blood vessel immaturity	752:774	blood vessel immaturity	752:774	Placenta from obese mothers showed blood vessel immaturity, hypoxia, increased transcript levels of inflammation, autophagy and altered levels of endoplasmic reticulum stress markers.
31081119	2	114	theme	inflammation	817:828	arg1	levels					807:812	increased transcript levels	786:812	increased transcript levels of inflammation, autophagy and altered levels of endoplasmic reticulum stress markers	786:898	Placenta from obese mothers showed blood vessel immaturity, hypoxia, increased transcript levels of inflammation, autophagy and altered levels of endoplasmic reticulum stress markers.
31081119	9	115	theme	acylcarnitine	2222:2234	arg1	derivatives					2236:2246	acylcarnitine derivatives	2222:2246	acylcarnitine derivatives	2222:2246	Placentas from HFD dams demonstrated blood vessel immaturity and hypoxia; decreased free carnitine, acylcarnitine derivatives and trimethylamine-N-oxide; and altered mRNA levels of inflammation, autophagy, and ER stress markers.
31081119	0	116	theme	fetal	129:133	arg1	proteins					147:154	altered fetal gut barrier proteins	121:154	altered fetal gut barrier proteins	121:154	High-fat diet intake modulates maternal intestinal adaptations to pregnancy and results in placental hypoxia, as well as altered fetal gut barrier proteins and immune markers.
31081119	1	117	theme	pro-inflammatory	375:390	arg1	mediators					392:400	pro-inflammatory mediators We investigated the impacts of diet-induced obesity in mice on maternal and fetal intestinal structure and placental vascularization Diet-induced obesity decreased maternal intestinal short chain fatty acids and their receptors, impaired gut barrier integrity and was associated with fetal intestinal inflammation	375:714	pro-inflammatory mediators We investigated the impacts of diet-induced obesity in mice on maternal and fetal intestinal structure and placental vascularization Diet-induced obesity decreased maternal intestinal short chain fatty acids and their receptors, impaired gut barrier integrity and was associated with fetal intestinal inflammation	375:714	KEY POINTS Maternal obesity has been associated with shifts in intestinal microbiota, which may contribute to impaired barrier function Impaired barrier function may expose the placenta and fetus to pro-inflammatory mediators We investigated the impacts of diet-induced obesity in mice on maternal and fetal intestinal structure and placental vascularization Diet-induced obesity decreased maternal intestinal short chain fatty acids and their receptors, impaired gut barrier integrity and was associated with fetal intestinal inflammation.
31081119	2	118	theme	blood	752:756	arg1	immaturity					765:774	blood vessel immaturity	752:774	blood vessel immaturity	752:774	Placenta from obese mothers showed blood vessel immaturity, hypoxia, increased transcript levels of inflammation, autophagy and altered levels of endoplasmic reticulum stress markers.
31081119	2	118	theme	blood	752:756	arg1	levels					807:812	increased transcript levels	786:812	increased transcript levels of inflammation, autophagy and altered levels of endoplasmic reticulum stress markers	786:898	Placenta from obese mothers showed blood vessel immaturity, hypoxia, increased transcript levels of inflammation, autophagy and altered levels of endoplasmic reticulum stress markers.
31081119	2	118	theme	blood	752:756	arg1	hypoxia					777:783	hypoxia	777:783	hypoxia	777:783	Placenta from obese mothers showed blood vessel immaturity, hypoxia, increased transcript levels of inflammation, autophagy and altered levels of endoplasmic reticulum stress markers.
31081119	0	119	theme	barrier	139:145	arg1	proteins					147:154	altered fetal gut barrier proteins	121:154	altered fetal gut barrier proteins	121:154	High-fat diet intake modulates maternal intestinal adaptations to pregnancy and results in placental hypoxia, as well as altered fetal gut barrier proteins and immune markers.
31081119	10	120	theme	factor-kappa	2407:2418	arg1	B					2420:2420	nuclear factor-kappa B	2399:2420	nuclear factor-kappa B	2399:2420	HFD exposed fetuses had increased activation of nuclear factor-kappa B and inhibition of the unfolded protein response in the developing intestine.
31081119	7	121	theme	SCFA	1792:1795	arg1	Gpr41					1806:1810	SCFA receptor Gpr41	1792:1810	SCFA receptor Gpr41	1792:1810	These diet-induced shifts paralleled decreased maternal intestinal mRNA levels of SCFA receptor Gpr41, modestly decreased cecal butyrate, and altered mRNA levels of inflammatory cytokines and immune cell markers in the maternal intestine.
31081119	8	122	theme	gut	1983:1985	arg1	integrity					1995:2003	impaired gut barrier integrity	1974:2003	impaired gut barrier integrity	1974:2003	Maternal HFD resulted in impaired gut barrier integrity, with corresponding increases in circulating maternal levels of lipopolysaccharide (LPS) and tumour necrosis factor.
31081119	7	123	from	cytokines	1888:1896	arg1	intestine					1938:1946	the maternal intestine	1925:1946	the maternal intestine	1925:1946	These diet-induced shifts paralleled decreased maternal intestinal mRNA levels of SCFA receptor Gpr41, modestly decreased cecal butyrate, and altered mRNA levels of inflammatory cytokines and immune cell markers in the maternal intestine.
31081119	8	124	from	increases	2025:2033	arg1	levels					2059:2064	circulating maternal levels	2038:2064	circulating maternal levels of lipopolysaccharide (LPS) and tumour necrosis factor	2038:2119	Maternal HFD resulted in impaired gut barrier integrity, with corresponding increases in circulating maternal levels of lipopolysaccharide (LPS) and tumour necrosis factor.
31081119	9	125	theme	altered	2280:2286	arg1	levels					2293:2298	altered mRNA levels	2280:2298	altered mRNA levels of inflammation, autophagy, and ER stress markers	2280:2348	Placentas from HFD dams demonstrated blood vessel immaturity and hypoxia; decreased free carnitine, acylcarnitine derivatives and trimethylamine-N-oxide; and altered mRNA levels of inflammation, autophagy, and ER stress markers.
31081119	1	126	from	impacts	422:428	arg1	mice					457:460	mice	457:460	mice	457:460	KEY POINTS Maternal obesity has been associated with shifts in intestinal microbiota, which may contribute to impaired barrier function Impaired barrier function may expose the placenta and fetus to pro-inflammatory mediators We investigated the impacts of diet-induced obesity in mice on maternal and fetal intestinal structure and placental vascularization Diet-induced obesity decreased maternal intestinal short chain fatty acids and their receptors, impaired gut barrier integrity and was associated with fetal intestinal inflammation.
31081119	1	126	from	impacts	422:428	arg1	structure					495:503	intestinal structure	484:503	intestinal structure	484:503	KEY POINTS Maternal obesity has been associated with shifts in intestinal microbiota, which may contribute to impaired barrier function Impaired barrier function may expose the placenta and fetus to pro-inflammatory mediators We investigated the impacts of diet-induced obesity in mice on maternal and fetal intestinal structure and placental vascularization Diet-induced obesity decreased maternal intestinal short chain fatty acids and their receptors, impaired gut barrier integrity and was associated with fetal intestinal inflammation.
31081119	1	126	from	impacts	422:428	arg1	vascularization					519:533	placental vascularization	509:533	placental vascularization	509:533	KEY POINTS Maternal obesity has been associated with shifts in intestinal microbiota, which may contribute to impaired barrier function Impaired barrier function may expose the placenta and fetus to pro-inflammatory mediators We investigated the impacts of diet-induced obesity in mice on maternal and fetal intestinal structure and placental vascularization Diet-induced obesity decreased maternal intestinal short chain fatty acids and their receptors, impaired gut barrier integrity and was associated with fetal intestinal inflammation.
31081119	5	127	theme	%	1304:1304	arg1	fat					1311:1313	17% kcal fat	1302:1313	17% kcal fat	1302:1313	In the present study, we generated cohorts of female C57BL/6J mice fed a control (17% kcal fat, n = 10-14) or a high-fat diet (HFD 60% kcal from fat, n = 10-14; ad libitum) aiming to investigate the impact on the maternal gut microbiota, intestinal inflammation and gut barrier integrity, placental inflammation and fetal intestinal development at embryonic day 18.5.
31081119	5	127	theme	%	1304:1304	arg1	n = 10-14					1316:1324	n = 10-14	1316:1324	n = 10-14	1316:1324	In the present study, we generated cohorts of female C57BL/6J mice fed a control (17% kcal fat, n = 10-14) or a high-fat diet (HFD 60% kcal from fat, n = 10-14; ad libitum) aiming to investigate the impact on the maternal gut microbiota, intestinal inflammation and gut barrier integrity, placental inflammation and fetal intestinal development at embryonic day 18.5.
31081119	2	128	theme	levels	853:858	arg1	immaturity					765:774	blood vessel immaturity	752:774	blood vessel immaturity	752:774	Placenta from obese mothers showed blood vessel immaturity, hypoxia, increased transcript levels of inflammation, autophagy and altered levels of endoplasmic reticulum stress markers.
31081119	2	128	theme	levels	853:858	arg1	levels					807:812	increased transcript levels	786:812	increased transcript levels of inflammation, autophagy and altered levels of endoplasmic reticulum stress markers	786:898	Placenta from obese mothers showed blood vessel immaturity, hypoxia, increased transcript levels of inflammation, autophagy and altered levels of endoplasmic reticulum stress markers.
31081119	1	129	theme	barrier	644:650	arg1	integrity					652:660	gut barrier integrity	640:660	gut barrier integrity	640:660	KEY POINTS Maternal obesity has been associated with shifts in intestinal microbiota, which may contribute to impaired barrier function Impaired barrier function may expose the placenta and fetus to pro-inflammatory mediators We investigated the impacts of diet-induced obesity in mice on maternal and fetal intestinal structure and placental vascularization Diet-induced obesity decreased maternal intestinal short chain fatty acids and their receptors, impaired gut barrier integrity and was associated with fetal intestinal inflammation.
31081119	8	130	theme	corresponding	2011:2023	arg1	increases					2025:2033	corresponding increases	2011:2033	corresponding increases in circulating maternal levels of lipopolysaccharide (LPS) and tumour necrosis factor	2011:2119	Maternal HFD resulted in impaired gut barrier integrity, with corresponding increases in circulating maternal levels of lipopolysaccharide (LPS) and tumour necrosis factor.
31081119	4	131	from	Shifts	1119:1124	arg1	microbiota					1149:1158	maternal intestinal microbiota	1129:1158	maternal intestinal microbiota	1129:1158	ABSTRACT Shifts in maternal intestinal microbiota have been implicated in metabolic adaptations to pregnancy.
31081119	7	132	theme	decreased	1747:1755	arg1	levels					1782:1787	decreased maternal intestinal mRNA levels	1747:1787	decreased maternal intestinal mRNA levels of SCFA receptor Gpr41	1747:1810	These diet-induced shifts paralleled decreased maternal intestinal mRNA levels of SCFA receptor Gpr41, modestly decreased cecal butyrate, and altered mRNA levels of inflammatory cytokines and immune cell markers in the maternal intestine.
31081119	7	133	from	markers	1914:1920	arg1	intestine					1938:1946	the maternal intestine	1925:1946	the maternal intestine	1925:1946	These diet-induced shifts paralleled decreased maternal intestinal mRNA levels of SCFA receptor Gpr41, modestly decreased cecal butyrate, and altered mRNA levels of inflammatory cytokines and immune cell markers in the maternal intestine.
31081119	5	134	theme	gut	1486:1488	arg1	integrity					1498:1506	gut barrier integrity	1486:1506	gut barrier integrity	1486:1506	In the present study, we generated cohorts of female C57BL/6J mice fed a control (17% kcal fat, n = 10-14) or a high-fat diet (HFD 60% kcal from fat, n = 10-14; ad libitum) aiming to investigate the impact on the maternal gut microbiota, intestinal inflammation and gut barrier integrity, placental inflammation and fetal intestinal development at embryonic day 18.5.
31081119	9	135	theme	inflammation	2303:2314	arg1	hypoxia					2187:2193	hypoxia	2187:2193	hypoxia	2187:2193	Placentas from HFD dams demonstrated blood vessel immaturity and hypoxia; decreased free carnitine, acylcarnitine derivatives and trimethylamine-N-oxide; and altered mRNA levels of inflammation, autophagy, and ER stress markers.
31081119	9	135	theme	inflammation	2303:2314	arg1	levels					2293:2298	altered mRNA levels	2280:2298	altered mRNA levels of inflammation, autophagy, and ER stress markers	2280:2348	Placentas from HFD dams demonstrated blood vessel immaturity and hypoxia; decreased free carnitine, acylcarnitine derivatives and trimethylamine-N-oxide; and altered mRNA levels of inflammation, autophagy, and ER stress markers.
31081119	9	135	theme	inflammation	2303:2314	arg1	derivatives					2236:2246	acylcarnitine derivatives	2222:2246	acylcarnitine derivatives	2222:2246	Placentas from HFD dams demonstrated blood vessel immaturity and hypoxia; decreased free carnitine, acylcarnitine derivatives and trimethylamine-N-oxide; and altered mRNA levels of inflammation, autophagy, and ER stress markers.
31081119	9	135	theme	inflammation	2303:2314	arg1	trimethylamine-N-oxide					2252:2273	trimethylamine-N-oxide	2252:2273	trimethylamine-N-oxide	2252:2273	Placentas from HFD dams demonstrated blood vessel immaturity and hypoxia; decreased free carnitine, acylcarnitine derivatives and trimethylamine-N-oxide; and altered mRNA levels of inflammation, autophagy, and ER stress markers.
31081119	9	135	theme	inflammation	2303:2314	arg1	immaturity					2172:2181	blood vessel immaturity	2159:2181	blood vessel immaturity	2159:2181	Placentas from HFD dams demonstrated blood vessel immaturity and hypoxia; decreased free carnitine, acylcarnitine derivatives and trimethylamine-N-oxide; and altered mRNA levels of inflammation, autophagy, and ER stress markers.
31081119	9	135	theme	inflammation	2303:2314	arg1	carnitine					2211:2219	decreased free carnitine	2196:2219	decreased free carnitine	2196:2219	Placentas from HFD dams demonstrated blood vessel immaturity and hypoxia; decreased free carnitine, acylcarnitine derivatives and trimethylamine-N-oxide; and altered mRNA levels of inflammation, autophagy, and ER stress markers.
31081119	6	136	theme	acid	1663:1666	arg1	abundances					1631:1640	decreased relative abundances	1612:1640	decreased relative abundances of short-chain fatty acid (SCFA) producing genera during pregnancy	1612:1707	HFD was associated with decreased relative abundances of short-chain fatty acid (SCFA) producing genera during pregnancy.
31081119	3	137	theme	maternal	925:932	arg1	changes					945:951	maternal intestinal changes	925:951	maternal intestinal changes	925:951	These data suggest that maternal intestinal changes probably contribute to adverse placental adaptations and also impart an increased risk of obesity in the offspring via alterations in fetal gut development.
31081119	10	138	theme	response	2461:2468	arg1	activation					2385:2394	increased activation	2375:2394	increased activation of nuclear factor-kappa B	2375:2420	HFD exposed fetuses had increased activation of nuclear factor-kappa B and inhibition of the unfolded protein response in the developing intestine.
31081119	10	138	theme	response	2461:2468	arg1	inhibition					2426:2435	inhibition	2426:2435	inhibition of the unfolded protein response in the developing intestine	2426:2496	HFD exposed fetuses had increased activation of nuclear factor-kappa B and inhibition of the unfolded protein response in the developing intestine.
31081119	1	139	theme	maternal	566:573	arg1	acids					604:608	maternal intestinal short chain fatty acids	566:608	maternal intestinal short chain fatty acids	566:608	KEY POINTS Maternal obesity has been associated with shifts in intestinal microbiota, which may contribute to impaired barrier function Impaired barrier function may expose the placenta and fetus to pro-inflammatory mediators We investigated the impacts of diet-induced obesity in mice on maternal and fetal intestinal structure and placental vascularization Diet-induced obesity decreased maternal intestinal short chain fatty acids and their receptors, impaired gut barrier integrity and was associated with fetal intestinal inflammation.
31081119	9	140	theme	autophagy	2317:2325	arg1	hypoxia					2187:2193	hypoxia	2187:2193	hypoxia	2187:2193	Placentas from HFD dams demonstrated blood vessel immaturity and hypoxia; decreased free carnitine, acylcarnitine derivatives and trimethylamine-N-oxide; and altered mRNA levels of inflammation, autophagy, and ER stress markers.
31081119	9	140	theme	autophagy	2317:2325	arg1	levels					2293:2298	altered mRNA levels	2280:2298	altered mRNA levels of inflammation, autophagy, and ER stress markers	2280:2348	Placentas from HFD dams demonstrated blood vessel immaturity and hypoxia; decreased free carnitine, acylcarnitine derivatives and trimethylamine-N-oxide; and altered mRNA levels of inflammation, autophagy, and ER stress markers.
31081119	9	140	theme	autophagy	2317:2325	arg1	derivatives					2236:2246	acylcarnitine derivatives	2222:2246	acylcarnitine derivatives	2222:2246	Placentas from HFD dams demonstrated blood vessel immaturity and hypoxia; decreased free carnitine, acylcarnitine derivatives and trimethylamine-N-oxide; and altered mRNA levels of inflammation, autophagy, and ER stress markers.
31081119	9	140	theme	autophagy	2317:2325	arg1	trimethylamine-N-oxide					2252:2273	trimethylamine-N-oxide	2252:2273	trimethylamine-N-oxide	2252:2273	Placentas from HFD dams demonstrated blood vessel immaturity and hypoxia; decreased free carnitine, acylcarnitine derivatives and trimethylamine-N-oxide; and altered mRNA levels of inflammation, autophagy, and ER stress markers.
31081119	9	140	theme	autophagy	2317:2325	arg1	immaturity					2172:2181	blood vessel immaturity	2159:2181	blood vessel immaturity	2159:2181	Placentas from HFD dams demonstrated blood vessel immaturity and hypoxia; decreased free carnitine, acylcarnitine derivatives and trimethylamine-N-oxide; and altered mRNA levels of inflammation, autophagy, and ER stress markers.
31081119	9	140	theme	autophagy	2317:2325	arg1	carnitine					2211:2219	decreased free carnitine	2196:2219	decreased free carnitine	2196:2219	Placentas from HFD dams demonstrated blood vessel immaturity and hypoxia; decreased free carnitine, acylcarnitine derivatives and trimethylamine-N-oxide; and altered mRNA levels of inflammation, autophagy, and ER stress markers.
31081119	8	141	theme	circulating	2038:2048	arg1	levels					2059:2064	circulating maternal levels	2038:2064	circulating maternal levels of lipopolysaccharide (LPS) and tumour necrosis factor	2038:2119	Maternal HFD resulted in impaired gut barrier integrity, with corresponding increases in circulating maternal levels of lipopolysaccharide (LPS) and tumour necrosis factor.
31081119	6	142	theme	short-chain	1645:1655	arg1	SCFA					1669:1672	SCFA	1669:1672	SCFA	1669:1672	HFD was associated with decreased relative abundances of short-chain fatty acid (SCFA) producing genera during pregnancy.
31081119	6	142	theme	short-chain	1645:1655	arg1	acid					1663:1666	short-chain fatty acid	1645:1666	short-chain fatty acid (SCFA) producing genera during pregnancy	1645:1707	HFD was associated with decreased relative abundances of short-chain fatty acid (SCFA) producing genera during pregnancy.
31081119	3	143	theme	adverse	976:982	arg1	adaptations					994:1004	adverse placental adaptations	976:1004	adverse placental adaptations	976:1004	These data suggest that maternal intestinal changes probably contribute to adverse placental adaptations and also impart an increased risk of obesity in the offspring via alterations in fetal gut development.
31081119	7	144	from	butyrate	1838:1845	arg1	intestine					1938:1946	the maternal intestine	1925:1946	the maternal intestine	1925:1946	These diet-induced shifts paralleled decreased maternal intestinal mRNA levels of SCFA receptor Gpr41, modestly decreased cecal butyrate, and altered mRNA levels of inflammatory cytokines and immune cell markers in the maternal intestine.
31081119	10	145	theme	unfolded	2444:2451	arg1	response					2461:2468	the unfolded protein response	2440:2468	the unfolded protein response in the developing intestine	2440:2496	HFD exposed fetuses had increased activation of nuclear factor-kappa B and inhibition of the unfolded protein response in the developing intestine.
31081119	1	146	theme	short	586:590	arg1	acids					604:608	maternal intestinal short chain fatty acids	566:608	maternal intestinal short chain fatty acids	566:608	KEY POINTS Maternal obesity has been associated with shifts in intestinal microbiota, which may contribute to impaired barrier function Impaired barrier function may expose the placenta and fetus to pro-inflammatory mediators We investigated the impacts of diet-induced obesity in mice on maternal and fetal intestinal structure and placental vascularization Diet-induced obesity decreased maternal intestinal short chain fatty acids and their receptors, impaired gut barrier integrity and was associated with fetal intestinal inflammation.
31081119	0	147	theme	diet	9:12	arg1	intake					14:19	High-fat diet intake	0:19	High-fat diet intake	0:19	High-fat diet intake modulates maternal intestinal adaptations to pregnancy and results in placental hypoxia, as well as altered fetal gut barrier proteins and immune markers.
31081119	11	148	theme	gut	2798:2800	arg1	development					2802:2812	fetal gut development	2792:2812	fetal gut development	2792:2812	Taken together, these data suggest that HFD intake prior to and during pregnancy shifts the composition of the maternal gut microbiota and impairs gut barrier integrity, resulting in increased maternal circulating LPS, which may ultimate contribute to changes in placental vascularization and fetal gut development.
31081119	1	149	dep	structure	495:503	arg1	obesity					548:554	Diet-induced obesity	535:554	Diet-induced obesity	535:554	KEY POINTS Maternal obesity has been associated with shifts in intestinal microbiota, which may contribute to impaired barrier function Impaired barrier function may expose the placenta and fetus to pro-inflammatory mediators We investigated the impacts of diet-induced obesity in mice on maternal and fetal intestinal structure and placental vascularization Diet-induced obesity decreased maternal intestinal short chain fatty acids and their receptors, impaired gut barrier integrity and was associated with fetal intestinal inflammation.
31081119	5	150	from	fat	1365:1367	arg1	 kcal					1354:1358	 kcal	1354:1358	 kcal	1354:1358	In the present study, we generated cohorts of female C57BL/6J mice fed a control (17% kcal fat, n = 10-14) or a high-fat diet (HFD 60% kcal from fat, n = 10-14; ad libitum) aiming to investigate the impact on the maternal gut microbiota, intestinal inflammation and gut barrier integrity, placental inflammation and fetal intestinal development at embryonic day 18.5.
31081119	11	151	theme	increased	2682:2690	arg1	LPS					2713:2715	increased maternal circulating LPS	2682:2715	increased maternal circulating LPS	2682:2715	Taken together, these data suggest that HFD intake prior to and during pregnancy shifts the composition of the maternal gut microbiota and impairs gut barrier integrity, resulting in increased maternal circulating LPS, which may ultimate contribute to changes in placental vascularization and fetal gut development.
31081119	1	152	theme	fatty	598:602	arg1	acids					604:608	maternal intestinal short chain fatty acids	566:608	maternal intestinal short chain fatty acids	566:608	KEY POINTS Maternal obesity has been associated with shifts in intestinal microbiota, which may contribute to impaired barrier function Impaired barrier function may expose the placenta and fetus to pro-inflammatory mediators We investigated the impacts of diet-induced obesity in mice on maternal and fetal intestinal structure and placental vascularization Diet-induced obesity decreased maternal intestinal short chain fatty acids and their receptors, impaired gut barrier integrity and was associated with fetal intestinal inflammation.
31081119	9	153	theme	stress	2335:2340	arg1	hypoxia					2187:2193	hypoxia	2187:2193	hypoxia	2187:2193	Placentas from HFD dams demonstrated blood vessel immaturity and hypoxia; decreased free carnitine, acylcarnitine derivatives and trimethylamine-N-oxide; and altered mRNA levels of inflammation, autophagy, and ER stress markers.
31081119	9	153	theme	stress	2335:2340	arg1	levels					2293:2298	altered mRNA levels	2280:2298	altered mRNA levels of inflammation, autophagy, and ER stress markers	2280:2348	Placentas from HFD dams demonstrated blood vessel immaturity and hypoxia; decreased free carnitine, acylcarnitine derivatives and trimethylamine-N-oxide; and altered mRNA levels of inflammation, autophagy, and ER stress markers.
31081119	9	153	theme	stress	2335:2340	arg1	derivatives					2236:2246	acylcarnitine derivatives	2222:2246	acylcarnitine derivatives	2222:2246	Placentas from HFD dams demonstrated blood vessel immaturity and hypoxia; decreased free carnitine, acylcarnitine derivatives and trimethylamine-N-oxide; and altered mRNA levels of inflammation, autophagy, and ER stress markers.
31081119	9	153	theme	stress	2335:2340	arg1	trimethylamine-N-oxide					2252:2273	trimethylamine-N-oxide	2252:2273	trimethylamine-N-oxide	2252:2273	Placentas from HFD dams demonstrated blood vessel immaturity and hypoxia; decreased free carnitine, acylcarnitine derivatives and trimethylamine-N-oxide; and altered mRNA levels of inflammation, autophagy, and ER stress markers.
31081119	9	153	theme	stress	2335:2340	arg1	immaturity					2172:2181	blood vessel immaturity	2159:2181	blood vessel immaturity	2159:2181	Placentas from HFD dams demonstrated blood vessel immaturity and hypoxia; decreased free carnitine, acylcarnitine derivatives and trimethylamine-N-oxide; and altered mRNA levels of inflammation, autophagy, and ER stress markers.
31081119	9	153	theme	stress	2335:2340	arg1	carnitine					2211:2219	decreased free carnitine	2196:2219	decreased free carnitine	2196:2219	Placentas from HFD dams demonstrated blood vessel immaturity and hypoxia; decreased free carnitine, acylcarnitine derivatives and trimethylamine-N-oxide; and altered mRNA levels of inflammation, autophagy, and ER stress markers.
31081119	8	154	theme	lipopolysaccharide	2069:2086	arg1	levels					2059:2064	circulating maternal levels	2038:2064	circulating maternal levels of lipopolysaccharide (LPS) and tumour necrosis factor	2038:2119	Maternal HFD resulted in impaired gut barrier integrity, with corresponding increases in circulating maternal levels of lipopolysaccharide (LPS) and tumour necrosis factor.
31081119	6	155	theme	decreased	1612:1620	arg1	abundances					1631:1640	decreased relative abundances	1612:1640	decreased relative abundances of short-chain fatty acid (SCFA) producing genera during pregnancy	1612:1707	HFD was associated with decreased relative abundances of short-chain fatty acid (SCFA) producing genera during pregnancy.
31081119	7	156	theme	altered	1852:1858	arg1	levels					1865:1870	altered mRNA levels	1852:1870	altered mRNA levels of inflammatory cytokines and immune cell markers in the maternal intestine	1852:1946	These diet-induced shifts paralleled decreased maternal intestinal mRNA levels of SCFA receptor Gpr41, modestly decreased cecal butyrate, and altered mRNA levels of inflammatory cytokines and immune cell markers in the maternal intestine.
31081119	5	157	theme	intestinal	1542:1551	arg1	development					1553:1563	fetal intestinal development	1536:1563	fetal intestinal development	1536:1563	In the present study, we generated cohorts of female C57BL/6J mice fed a control (17% kcal fat, n = 10-14) or a high-fat diet (HFD 60% kcal from fat, n = 10-14; ad libitum) aiming to investigate the impact on the maternal gut microbiota, intestinal inflammation and gut barrier integrity, placental inflammation and fetal intestinal development at embryonic day 18.5.
31081119	0	158	theme	intestinal	40:49	arg1	adaptations					51:61	maternal intestinal adaptations	31:61	maternal intestinal adaptations to pregnancy	31:74	High-fat diet intake modulates maternal intestinal adaptations to pregnancy and results in placental hypoxia, as well as altered fetal gut barrier proteins and immune markers.
31081119	7	159	theme	markers	1914:1920	arg1	levels					1865:1870	altered mRNA levels	1852:1870	altered mRNA levels of inflammatory cytokines and immune cell markers in the maternal intestine	1852:1946	These diet-induced shifts paralleled decreased maternal intestinal mRNA levels of SCFA receptor Gpr41, modestly decreased cecal butyrate, and altered mRNA levels of inflammatory cytokines and immune cell markers in the maternal intestine.
31081119	7	159	theme	markers	1914:1920	arg1	butyrate					1838:1845	modestly decreased cecal butyrate	1813:1845	modestly decreased cecal butyrate	1813:1845	These diet-induced shifts paralleled decreased maternal intestinal mRNA levels of SCFA receptor Gpr41, modestly decreased cecal butyrate, and altered mRNA levels of inflammatory cytokines and immune cell markers in the maternal intestine.
31081119	7	159	theme	markers	1914:1920	arg1	levels					1782:1787	decreased maternal intestinal mRNA levels	1747:1787	decreased maternal intestinal mRNA levels of SCFA receptor Gpr41	1747:1810	These diet-induced shifts paralleled decreased maternal intestinal mRNA levels of SCFA receptor Gpr41, modestly decreased cecal butyrate, and altered mRNA levels of inflammatory cytokines and immune cell markers in the maternal intestine.
31081119	8	160	theme	necrosis	2105:2112	arg1	factor					2114:2119	tumour necrosis factor	2098:2119	tumour necrosis factor	2098:2119	Maternal HFD resulted in impaired gut barrier integrity, with corresponding increases in circulating maternal levels of lipopolysaccharide (LPS) and tumour necrosis factor.
31081119	11	161	theme	gut	2619:2621	arg1	microbiota					2623:2632	the maternal gut microbiota	2606:2632	the maternal gut microbiota	2606:2632	Taken together, these data suggest that HFD intake prior to and during pregnancy shifts the composition of the maternal gut microbiota and impairs gut barrier integrity, resulting in increased maternal circulating LPS, which may ultimate contribute to changes in placental vascularization and fetal gut development.
31081119	5	162	theme	ad	1381:1382	arg1	libitum					1384:1390	ad libitum	1381:1390	ad libitum	1381:1390	In the present study, we generated cohorts of female C57BL/6J mice fed a control (17% kcal fat, n = 10-14) or a high-fat diet (HFD 60% kcal from fat, n = 10-14; ad libitum) aiming to investigate the impact on the maternal gut microbiota, intestinal inflammation and gut barrier integrity, placental inflammation and fetal intestinal development at embryonic day 18.5.
31081119	1	163	theme	barrier	295:301	arg1	function					303:310	impaired barrier function	286:310	impaired barrier function	286:310	KEY POINTS Maternal obesity has been associated with shifts in intestinal microbiota, which may contribute to impaired barrier function Impaired barrier function may expose the placenta and fetus to pro-inflammatory mediators We investigated the impacts of diet-induced obesity in mice on maternal and fetal intestinal structure and placental vascularization Diet-induced obesity decreased maternal intestinal short chain fatty acids and their receptors, impaired gut barrier integrity and was associated with fetal intestinal inflammation.
31081119	7	164	theme	immune	1902:1907	arg1	markers					1914:1920	immune cell markers	1902:1920	immune cell markers in the maternal intestine	1902:1946	These diet-induced shifts paralleled decreased maternal intestinal mRNA levels of SCFA receptor Gpr41, modestly decreased cecal butyrate, and altered mRNA levels of inflammatory cytokines and immune cell markers in the maternal intestine.
31081119	7	165	theme	inflammatory	1875:1886	arg1	cytokines					1888:1896	inflammatory cytokines	1875:1896	inflammatory cytokines	1875:1896	These diet-induced shifts paralleled decreased maternal intestinal mRNA levels of SCFA receptor Gpr41, modestly decreased cecal butyrate, and altered mRNA levels of inflammatory cytokines and immune cell markers in the maternal intestine.
31081119	0	166	theme	altered	121:127	arg1	proteins					147:154	altered fetal gut barrier proteins	121:154	altered fetal gut barrier proteins	121:154	High-fat diet intake modulates maternal intestinal adaptations to pregnancy and results in placental hypoxia, as well as altered fetal gut barrier proteins and immune markers.
31081119	11	167	theme	circulating	2701:2711	arg1	LPS					2713:2715	increased maternal circulating LPS	2682:2715	increased maternal circulating LPS	2682:2715	Taken together, these data suggest that HFD intake prior to and during pregnancy shifts the composition of the maternal gut microbiota and impairs gut barrier integrity, resulting in increased maternal circulating LPS, which may ultimate contribute to changes in placental vascularization and fetal gut development.
31081119	5	168	theme	placental	1509:1517	arg1	inflammation					1519:1530	placental inflammation	1509:1530	placental inflammation	1509:1530	In the present study, we generated cohorts of female C57BL/6J mice fed a control (17% kcal fat, n = 10-14) or a high-fat diet (HFD 60% kcal from fat, n = 10-14; ad libitum) aiming to investigate the impact on the maternal gut microbiota, intestinal inflammation and gut barrier integrity, placental inflammation and fetal intestinal development at embryonic day 18.5.
31081119	10	169	contain	had	2371:2373	arg1	fetuses					2363:2369	HFD exposed fetuses	2351:2369	HFD exposed fetuses	2351:2369	HFD exposed fetuses had increased activation of nuclear factor-kappa B and inhibition of the unfolded protein response in the developing intestine.
31081119	10	169	contain	had	2371:2373	arg2	activation					2385:2394	increased activation	2375:2394	increased activation of nuclear factor-kappa B	2375:2420	HFD exposed fetuses had increased activation of nuclear factor-kappa B and inhibition of the unfolded protein response in the developing intestine.
31081119	10	169	contain	had	2371:2373	arg2	inhibition					2426:2435	inhibition	2426:2435	inhibition of the unfolded protein response in the developing intestine	2426:2496	HFD exposed fetuses had increased activation of nuclear factor-kappa B and inhibition of the unfolded protein response in the developing intestine.
31081119	1	170	theme	Impaired	312:319	arg1	function					329:336	Impaired barrier function	312:336	Impaired barrier function	312:336	KEY POINTS Maternal obesity has been associated with shifts in intestinal microbiota, which may contribute to impaired barrier function Impaired barrier function may expose the placenta and fetus to pro-inflammatory mediators We investigated the impacts of diet-induced obesity in mice on maternal and fetal intestinal structure and placental vascularization Diet-induced obesity decreased maternal intestinal short chain fatty acids and their receptors, impaired gut barrier integrity and was associated with fetal intestinal inflammation.
31081119	7	171	theme	cytokines	1888:1896	arg1	levels					1865:1870	altered mRNA levels	1852:1870	altered mRNA levels of inflammatory cytokines and immune cell markers in the maternal intestine	1852:1946	These diet-induced shifts paralleled decreased maternal intestinal mRNA levels of SCFA receptor Gpr41, modestly decreased cecal butyrate, and altered mRNA levels of inflammatory cytokines and immune cell markers in the maternal intestine.
31081119	7	171	theme	cytokines	1888:1896	arg1	butyrate					1838:1845	modestly decreased cecal butyrate	1813:1845	modestly decreased cecal butyrate	1813:1845	These diet-induced shifts paralleled decreased maternal intestinal mRNA levels of SCFA receptor Gpr41, modestly decreased cecal butyrate, and altered mRNA levels of inflammatory cytokines and immune cell markers in the maternal intestine.
31081119	7	171	theme	cytokines	1888:1896	arg1	levels					1782:1787	decreased maternal intestinal mRNA levels	1747:1787	decreased maternal intestinal mRNA levels of SCFA receptor Gpr41	1747:1810	These diet-induced shifts paralleled decreased maternal intestinal mRNA levels of SCFA receptor Gpr41, modestly decreased cecal butyrate, and altered mRNA levels of inflammatory cytokines and immune cell markers in the maternal intestine.
31081119	4	172	theme	intestinal	1138:1147	arg1	microbiota					1149:1158	maternal intestinal microbiota	1129:1158	maternal intestinal microbiota	1129:1158	ABSTRACT Shifts in maternal intestinal microbiota have been implicated in metabolic adaptations to pregnancy.
31081119	1	173	dep	mediators	392:400	arg1	investigated					405:416	investigated	405:416	investigated the impacts of diet-induced obesity in mice on maternal and fetal intestinal structure and placental vascularization Diet-induced obesity decreased maternal intestinal short chain fatty acids and their receptors, impaired gut barrier integrity and was associated with fetal intestinal inflammation	405:714	KEY POINTS Maternal obesity has been associated with shifts in intestinal microbiota, which may contribute to impaired barrier function Impaired barrier function may expose the placenta and fetus to pro-inflammatory mediators We investigated the impacts of diet-induced obesity in mice on maternal and fetal intestinal structure and placental vascularization Diet-induced obesity decreased maternal intestinal short chain fatty acids and their receptors, impaired gut barrier integrity and was associated with fetal intestinal inflammation.
31081119	9	174	theme	HFD	2137:2139	arg1	dams					2141:2144	HFD dams	2137:2144	HFD dams	2137:2144	Placentas from HFD dams demonstrated blood vessel immaturity and hypoxia; decreased free carnitine, acylcarnitine derivatives and trimethylamine-N-oxide; and altered mRNA levels of inflammation, autophagy, and ER stress markers.
31081119	5	175	theme	 kcal	1354:1358	arg1	%					1353:1353	60%	1351:1353	60% kcal from fat	1351:1367	In the present study, we generated cohorts of female C57BL/6J mice fed a control (17% kcal fat, n = 10-14) or a high-fat diet (HFD 60% kcal from fat, n = 10-14; ad libitum) aiming to investigate the impact on the maternal gut microbiota, intestinal inflammation and gut barrier integrity, placental inflammation and fetal intestinal development at embryonic day 18.5.
31081119	9	176	dep	inflammation	2303:2314	arg1	markers					2342:2348	markers	2342:2348	markers	2342:2348	Placentas from HFD dams demonstrated blood vessel immaturity and hypoxia; decreased free carnitine, acylcarnitine derivatives and trimethylamine-N-oxide; and altered mRNA levels of inflammation, autophagy, and ER stress markers.
31081119	5	177	dep	HFD	1347:1349	arg1	libitum					1384:1390	ad libitum	1381:1390	ad libitum	1381:1390	In the present study, we generated cohorts of female C57BL/6J mice fed a control (17% kcal fat, n = 10-14) or a high-fat diet (HFD 60% kcal from fat, n = 10-14; ad libitum) aiming to investigate the impact on the maternal gut microbiota, intestinal inflammation and gut barrier integrity, placental inflammation and fetal intestinal development at embryonic day 18.5.
31081119	5	177	dep	HFD	1347:1349	arg1	%					1353:1353	60%	1351:1353	60% kcal from fat	1351:1367	In the present study, we generated cohorts of female C57BL/6J mice fed a control (17% kcal fat, n = 10-14) or a high-fat diet (HFD 60% kcal from fat, n = 10-14; ad libitum) aiming to investigate the impact on the maternal gut microbiota, intestinal inflammation and gut barrier integrity, placental inflammation and fetal intestinal development at embryonic day 18.5.
31081119	10	178	from	activation	2385:2394	arg1	intestine					2488:2496	the developing intestine	2473:2496	the developing intestine	2473:2496	HFD exposed fetuses had increased activation of nuclear factor-kappa B and inhibition of the unfolded protein response in the developing intestine.
31081119	11	179	theme	microbiota	2623:2632	arg1	composition					2591:2601	the composition	2587:2601	the composition of the maternal gut microbiota	2587:2632	Taken together, these data suggest that HFD intake prior to and during pregnancy shifts the composition of the maternal gut microbiota and impairs gut barrier integrity, resulting in increased maternal circulating LPS, which may ultimate contribute to changes in placental vascularization and fetal gut development.
31081119	5	180	theme	17	1302:1303	arg1	%					1304:1304	%	1304:1304	%	1304:1304	In the present study, we generated cohorts of female C57BL/6J mice fed a control (17% kcal fat, n = 10-14) or a high-fat diet (HFD 60% kcal from fat, n = 10-14; ad libitum) aiming to investigate the impact on the maternal gut microbiota, intestinal inflammation and gut barrier integrity, placental inflammation and fetal intestinal development at embryonic day 18.5.
30047083	4	0	dep	5	453:453	arg1	to					450:451	to	450:451	to	450:451	The strain's growth temperature ranges from 1 to 35°C, with an optimal growth being observed at 28°C. Growth occurs from 0 to 5% NaCl and at pH 6-8, with optimal growth being observed in 1% NaCl at pH 7.
30047083	6	1	theme	SYP-B2174T	811:820	arg1	lower					879:883	lower	879:883	lower	879:883	The DNA-DNA hybridization values of the strain SYP-B2174T to its closest phylogenetic neighbors were significantly lower than 35.7%.
30047083	6	1	theme	SYP-B2174T	811:820	arg1	values					790:795	The DNA-DNA hybridization values	764:795	The DNA-DNA hybridization values of the strain SYP-B2174T to its closest phylogenetic neighbors	764:858	The DNA-DNA hybridization values of the strain SYP-B2174T to its closest phylogenetic neighbors were significantly lower than 35.7%.
30047083	7	2	theme	iso-C16:0	1213:1221	arg1	MK-11					1145:1149	major menaquinone MK-11	1127:1149	major menaquinone MK-11	1127:1149	The strain was identified as a novel species of the genus Leifsonia judging by the coryneform morphology, peptidoglycans based upon 2,4-diaminobutyric acid, principal phospholipids phosphatidylglycerol and diphosphatidylglycerol, major menaquinone MK-11, predominant fatty acids of anteiso-C15:0, anteiso-C17:0, and iso-C16:0, and a DNA G + C base composition of 68.7 mol%, for which the name Leifsonia flava sp.
30047083	7	2	theme	iso-C16:0	1213:1221	arg1	phospholipids					1064:1076	principal phospholipids	1054:1076	principal phospholipids phosphatidylglycerol and diphosphatidylglycerol, major menaquinone MK-11, predominant fatty acids of anteiso-C15:0, anteiso-C17:0, and iso-C16:0	1054:1221	The strain was identified as a novel species of the genus Leifsonia judging by the coryneform morphology, peptidoglycans based upon 2,4-diaminobutyric acid, principal phospholipids phosphatidylglycerol and diphosphatidylglycerol, major menaquinone MK-11, predominant fatty acids of anteiso-C15:0, anteiso-C17:0, and iso-C16:0, and a DNA G + C base composition of 68.7 mol%, for which the name Leifsonia flava sp.
30047083	7	2	theme	iso-C16:0	1213:1221	arg1	diphosphatidylglycerol					1103:1124	diphosphatidylglycerol	1103:1124	diphosphatidylglycerol	1103:1124	The strain was identified as a novel species of the genus Leifsonia judging by the coryneform morphology, peptidoglycans based upon 2,4-diaminobutyric acid, principal phospholipids phosphatidylglycerol and diphosphatidylglycerol, major menaquinone MK-11, predominant fatty acids of anteiso-C15:0, anteiso-C17:0, and iso-C16:0, and a DNA G + C base composition of 68.7 mol%, for which the name Leifsonia flava sp.
30047083	7	2	theme	iso-C16:0	1213:1221	arg1	acids					1170:1174	predominant fatty acids	1152:1174	predominant fatty acids	1152:1174	The strain was identified as a novel species of the genus Leifsonia judging by the coryneform morphology, peptidoglycans based upon 2,4-diaminobutyric acid, principal phospholipids phosphatidylglycerol and diphosphatidylglycerol, major menaquinone MK-11, predominant fatty acids of anteiso-C15:0, anteiso-C17:0, and iso-C16:0, and a DNA G + C base composition of 68.7 mol%, for which the name Leifsonia flava sp.
30047083	7	2	theme	iso-C16:0	1213:1221	arg1	phosphatidylglycerol					1078:1097	phosphatidylglycerol	1078:1097	phosphatidylglycerol	1078:1097	The strain was identified as a novel species of the genus Leifsonia judging by the coryneform morphology, peptidoglycans based upon 2,4-diaminobutyric acid, principal phospholipids phosphatidylglycerol and diphosphatidylglycerol, major menaquinone MK-11, predominant fatty acids of anteiso-C15:0, anteiso-C17:0, and iso-C16:0, and a DNA G + C base composition of 68.7 mol%, for which the name Leifsonia flava sp.
30047083	9	3	theme	type	1332:1335	arg1	strain					1337:1342	The type strain	1328:1342	The type strain	1328:1342	The type strain is SYP-B2174T (= CGMCC 1.15856T = DSM 105144T = KCTC 39963T).
30047083	9	3	theme	type	1332:1335	arg1	SYP-B2174T					1347:1356	SYP-B2174T	1347:1356	SYP-B2174T (= CGMCC 1.15856T = DSM 105144T = KCTC 39963T)	1347:1403	The type strain is SYP-B2174T (= CGMCC 1.15856T = DSM 105144T = KCTC 39963T).
30047083	5	4	dep	species	632:638	arg1	species					632:638	the species Leifsonia kafniensis JCM 17021T and Leifsonia psychrotolerans DSM 22824T	628:711	the species Leifsonia kafniensis JCM 17021T and Leifsonia psychrotolerans DSM 22824T	628:711	Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species Leifsonia kafniensis JCM 17021T and Leifsonia psychrotolerans DSM 22824T with similarities of 97.8 and 97.6%, respectively.
30047083	5	4	dep	species	632:638	arg1	22824T					706:711	Leifsonia psychrotolerans DSM 22824T	676:711	Leifsonia psychrotolerans DSM 22824T	676:711	Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species Leifsonia kafniensis JCM 17021T and Leifsonia psychrotolerans DSM 22824T with similarities of 97.8 and 97.6%, respectively.
30047083	5	4	dep	species	632:638	arg1	17021T					665:670	Leifsonia kafniensis JCM 17021T	640:670	Leifsonia kafniensis JCM 17021T	640:670	Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species Leifsonia kafniensis JCM 17021T and Leifsonia psychrotolerans DSM 22824T with similarities of 97.8 and 97.6%, respectively.
30047083	9	5	theme	1.15856T	1367:1374	arg1	39963T					1397:1402	= CGMCC 1.15856T = DSM 105144T = KCTC 39963T	1359:1402	= CGMCC 1.15856T = DSM 105144T = KCTC 39963T	1359:1402	The type strain is SYP-B2174T (= CGMCC 1.15856T = DSM 105144T = KCTC 39963T).
30047083	9	5	theme	1.15856T	1367:1374	arg1	SYP-B2174T					1347:1356	SYP-B2174T	1347:1356	SYP-B2174T (= CGMCC 1.15856T = DSM 105144T = KCTC 39963T)	1347:1403	The type strain is SYP-B2174T (= CGMCC 1.15856T = DSM 105144T = KCTC 39963T).
30047083	7	6	theme	principal	1054:1062	arg1	phospholipids					1064:1076	principal phospholipids	1054:1076	principal phospholipids phosphatidylglycerol and diphosphatidylglycerol, major menaquinone MK-11, predominant fatty acids of anteiso-C15:0, anteiso-C17:0, and iso-C16:0	1054:1221	The strain was identified as a novel species of the genus Leifsonia judging by the coryneform morphology, peptidoglycans based upon 2,4-diaminobutyric acid, principal phospholipids phosphatidylglycerol and diphosphatidylglycerol, major menaquinone MK-11, predominant fatty acids of anteiso-C15:0, anteiso-C17:0, and iso-C16:0, and a DNA G + C base composition of 68.7 mol%, for which the name Leifsonia flava sp.
30047083	7	6	theme	principal	1054:1062	arg1	diphosphatidylglycerol					1103:1124	diphosphatidylglycerol	1103:1124	diphosphatidylglycerol	1103:1124	The strain was identified as a novel species of the genus Leifsonia judging by the coryneform morphology, peptidoglycans based upon 2,4-diaminobutyric acid, principal phospholipids phosphatidylglycerol and diphosphatidylglycerol, major menaquinone MK-11, predominant fatty acids of anteiso-C15:0, anteiso-C17:0, and iso-C16:0, and a DNA G + C base composition of 68.7 mol%, for which the name Leifsonia flava sp.
30047083	7	6	theme	principal	1054:1062	arg1	phosphatidylglycerol					1078:1097	phosphatidylglycerol	1078:1097	phosphatidylglycerol	1078:1097	The strain was identified as a novel species of the genus Leifsonia judging by the coryneform morphology, peptidoglycans based upon 2,4-diaminobutyric acid, principal phospholipids phosphatidylglycerol and diphosphatidylglycerol, major menaquinone MK-11, predominant fatty acids of anteiso-C15:0, anteiso-C17:0, and iso-C16:0, and a DNA G + C base composition of 68.7 mol%, for which the name Leifsonia flava sp.
30047083	4	7	theme	%	515:515	arg1	NaCl					517:520	1% NaCl	514:520	1% NaCl at pH 7	514:528	The strain's growth temperature ranges from 1 to 35°C, with an optimal growth being observed at 28°C. Growth occurs from 0 to 5% NaCl and at pH 6-8, with optimal growth being observed in 1% NaCl at pH 7.
30047083	4	8	from	pH	525:526	arg1	NaCl					517:520	1% NaCl	514:520	1% NaCl at pH 7	514:528	The strain's growth temperature ranges from 1 to 35°C, with an optimal growth being observed at 28°C. Growth occurs from 0 to 5% NaCl and at pH 6-8, with optimal growth being observed in 1% NaCl at pH 7.
30047083	6	9	theme	phylogenetic	837:848	arg1	neighbors					850:858	its closest phylogenetic neighbors	825:858	its closest phylogenetic neighbors	825:858	The DNA-DNA hybridization values of the strain SYP-B2174T to its closest phylogenetic neighbors were significantly lower than 35.7%.
30047083	7	10	theme	flava	1300:1304	arg1	sp					1306:1307	the name Leifsonia flava sp	1281:1307	the name Leifsonia flava sp	1281:1307	The strain was identified as a novel species of the genus Leifsonia judging by the coryneform morphology, peptidoglycans based upon 2,4-diaminobutyric acid, principal phospholipids phosphatidylglycerol and diphosphatidylglycerol, major menaquinone MK-11, predominant fatty acids of anteiso-C15:0, anteiso-C17:0, and iso-C16:0, and a DNA G + C base composition of 68.7 mol%, for which the name Leifsonia flava sp.
30047083	7	11	theme	coryneform	980:989	arg1	morphology					991:1000	the coryneform morphology	976:1000	the coryneform morphology	976:1000	The strain was identified as a novel species of the genus Leifsonia judging by the coryneform morphology, peptidoglycans based upon 2,4-diaminobutyric acid, principal phospholipids phosphatidylglycerol and diphosphatidylglycerol, major menaquinone MK-11, predominant fatty acids of anteiso-C15:0, anteiso-C17:0, and iso-C16:0, and a DNA G + C base composition of 68.7 mol%, for which the name Leifsonia flava sp.
30047083	7	12	theme	anteiso-C15:0	1179:1191	arg1	MK-11					1145:1149	major menaquinone MK-11	1127:1149	major menaquinone MK-11	1127:1149	The strain was identified as a novel species of the genus Leifsonia judging by the coryneform morphology, peptidoglycans based upon 2,4-diaminobutyric acid, principal phospholipids phosphatidylglycerol and diphosphatidylglycerol, major menaquinone MK-11, predominant fatty acids of anteiso-C15:0, anteiso-C17:0, and iso-C16:0, and a DNA G + C base composition of 68.7 mol%, for which the name Leifsonia flava sp.
30047083	7	12	theme	anteiso-C15:0	1179:1191	arg1	phospholipids					1064:1076	principal phospholipids	1054:1076	principal phospholipids phosphatidylglycerol and diphosphatidylglycerol, major menaquinone MK-11, predominant fatty acids of anteiso-C15:0, anteiso-C17:0, and iso-C16:0	1054:1221	The strain was identified as a novel species of the genus Leifsonia judging by the coryneform morphology, peptidoglycans based upon 2,4-diaminobutyric acid, principal phospholipids phosphatidylglycerol and diphosphatidylglycerol, major menaquinone MK-11, predominant fatty acids of anteiso-C15:0, anteiso-C17:0, and iso-C16:0, and a DNA G + C base composition of 68.7 mol%, for which the name Leifsonia flava sp.
30047083	7	12	theme	anteiso-C15:0	1179:1191	arg1	diphosphatidylglycerol					1103:1124	diphosphatidylglycerol	1103:1124	diphosphatidylglycerol	1103:1124	The strain was identified as a novel species of the genus Leifsonia judging by the coryneform morphology, peptidoglycans based upon 2,4-diaminobutyric acid, principal phospholipids phosphatidylglycerol and diphosphatidylglycerol, major menaquinone MK-11, predominant fatty acids of anteiso-C15:0, anteiso-C17:0, and iso-C16:0, and a DNA G + C base composition of 68.7 mol%, for which the name Leifsonia flava sp.
30047083	7	12	theme	anteiso-C15:0	1179:1191	arg1	acids					1170:1174	predominant fatty acids	1152:1174	predominant fatty acids	1152:1174	The strain was identified as a novel species of the genus Leifsonia judging by the coryneform morphology, peptidoglycans based upon 2,4-diaminobutyric acid, principal phospholipids phosphatidylglycerol and diphosphatidylglycerol, major menaquinone MK-11, predominant fatty acids of anteiso-C15:0, anteiso-C17:0, and iso-C16:0, and a DNA G + C base composition of 68.7 mol%, for which the name Leifsonia flava sp.
30047083	7	12	theme	anteiso-C15:0	1179:1191	arg1	phosphatidylglycerol					1078:1097	phosphatidylglycerol	1078:1097	phosphatidylglycerol	1078:1097	The strain was identified as a novel species of the genus Leifsonia judging by the coryneform morphology, peptidoglycans based upon 2,4-diaminobutyric acid, principal phospholipids phosphatidylglycerol and diphosphatidylglycerol, major menaquinone MK-11, predominant fatty acids of anteiso-C15:0, anteiso-C17:0, and iso-C16:0, and a DNA G + C base composition of 68.7 mol%, for which the name Leifsonia flava sp.
30047083	6	13	theme	closest	829:835	arg1	neighbors					850:858	its closest phylogenetic neighbors	825:858	its closest phylogenetic neighbors	825:858	The DNA-DNA hybridization values of the strain SYP-B2174T to its closest phylogenetic neighbors were significantly lower than 35.7%.
30047083	7	14	theme	anteiso-C17:0	1194:1206	arg1	MK-11					1145:1149	major menaquinone MK-11	1127:1149	major menaquinone MK-11	1127:1149	The strain was identified as a novel species of the genus Leifsonia judging by the coryneform morphology, peptidoglycans based upon 2,4-diaminobutyric acid, principal phospholipids phosphatidylglycerol and diphosphatidylglycerol, major menaquinone MK-11, predominant fatty acids of anteiso-C15:0, anteiso-C17:0, and iso-C16:0, and a DNA G + C base composition of 68.7 mol%, for which the name Leifsonia flava sp.
30047083	7	14	theme	anteiso-C17:0	1194:1206	arg1	phospholipids					1064:1076	principal phospholipids	1054:1076	principal phospholipids phosphatidylglycerol and diphosphatidylglycerol, major menaquinone MK-11, predominant fatty acids of anteiso-C15:0, anteiso-C17:0, and iso-C16:0	1054:1221	The strain was identified as a novel species of the genus Leifsonia judging by the coryneform morphology, peptidoglycans based upon 2,4-diaminobutyric acid, principal phospholipids phosphatidylglycerol and diphosphatidylglycerol, major menaquinone MK-11, predominant fatty acids of anteiso-C15:0, anteiso-C17:0, and iso-C16:0, and a DNA G + C base composition of 68.7 mol%, for which the name Leifsonia flava sp.
30047083	7	14	theme	anteiso-C17:0	1194:1206	arg1	diphosphatidylglycerol					1103:1124	diphosphatidylglycerol	1103:1124	diphosphatidylglycerol	1103:1124	The strain was identified as a novel species of the genus Leifsonia judging by the coryneform morphology, peptidoglycans based upon 2,4-diaminobutyric acid, principal phospholipids phosphatidylglycerol and diphosphatidylglycerol, major menaquinone MK-11, predominant fatty acids of anteiso-C15:0, anteiso-C17:0, and iso-C16:0, and a DNA G + C base composition of 68.7 mol%, for which the name Leifsonia flava sp.
30047083	7	14	theme	anteiso-C17:0	1194:1206	arg1	acids					1170:1174	predominant fatty acids	1152:1174	predominant fatty acids	1152:1174	The strain was identified as a novel species of the genus Leifsonia judging by the coryneform morphology, peptidoglycans based upon 2,4-diaminobutyric acid, principal phospholipids phosphatidylglycerol and diphosphatidylglycerol, major menaquinone MK-11, predominant fatty acids of anteiso-C15:0, anteiso-C17:0, and iso-C16:0, and a DNA G + C base composition of 68.7 mol%, for which the name Leifsonia flava sp.
30047083	7	14	theme	anteiso-C17:0	1194:1206	arg1	phosphatidylglycerol					1078:1097	phosphatidylglycerol	1078:1097	phosphatidylglycerol	1078:1097	The strain was identified as a novel species of the genus Leifsonia judging by the coryneform morphology, peptidoglycans based upon 2,4-diaminobutyric acid, principal phospholipids phosphatidylglycerol and diphosphatidylglycerol, major menaquinone MK-11, predominant fatty acids of anteiso-C15:0, anteiso-C17:0, and iso-C16:0, and a DNA G + C base composition of 68.7 mol%, for which the name Leifsonia flava sp.
30047083	5	15	theme	Comparative	531:541	arg1	analysis					585:592	Comparative 16S rRNA gene sequence-based phylogenetic analysis	531:592	Comparative 16S rRNA gene sequence-based phylogenetic analysis	531:592	Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species Leifsonia kafniensis JCM 17021T and Leifsonia psychrotolerans DSM 22824T with similarities of 97.8 and 97.6%, respectively.
30047083	4	16	located	observed	502:509	arg2	growth					489:494	optimal growth	481:494	optimal growth	481:494	The strain's growth temperature ranges from 1 to 35°C, with an optimal growth being observed at 28°C. Growth occurs from 0 to 5% NaCl and at pH 6-8, with optimal growth being observed in 1% NaCl at pH 7.
30047083	4	16	located	observed	502:509	arg1	NaCl					517:520	1% NaCl	514:520	1% NaCl at pH 7	514:528	The strain's growth temperature ranges from 1 to 35°C, with an optimal growth being observed at 28°C. Growth occurs from 0 to 5% NaCl and at pH 6-8, with optimal growth being observed in 1% NaCl at pH 7.
30047083	9	17	theme	=	1376:1376	arg1	39963T					1397:1402	= CGMCC 1.15856T = DSM 105144T = KCTC 39963T	1359:1402	= CGMCC 1.15856T = DSM 105144T = KCTC 39963T	1359:1402	The type strain is SYP-B2174T (= CGMCC 1.15856T = DSM 105144T = KCTC 39963T).
30047083	9	17	theme	=	1376:1376	arg1	SYP-B2174T					1347:1356	SYP-B2174T	1347:1356	SYP-B2174T (= CGMCC 1.15856T = DSM 105144T = KCTC 39963T)	1347:1403	The type strain is SYP-B2174T (= CGMCC 1.15856T = DSM 105144T = KCTC 39963T).
30047083	7	18	theme	fatty	1164:1168	arg1	acids					1170:1174	predominant fatty acids	1152:1174	predominant fatty acids	1152:1174	The strain was identified as a novel species of the genus Leifsonia judging by the coryneform morphology, peptidoglycans based upon 2,4-diaminobutyric acid, principal phospholipids phosphatidylglycerol and diphosphatidylglycerol, major menaquinone MK-11, predominant fatty acids of anteiso-C15:0, anteiso-C17:0, and iso-C16:0, and a DNA G + C base composition of 68.7 mol%, for which the name Leifsonia flava sp.
30047083	7	18	theme	fatty	1164:1168	arg1	diphosphatidylglycerol					1103:1124	diphosphatidylglycerol	1103:1124	diphosphatidylglycerol	1103:1124	The strain was identified as a novel species of the genus Leifsonia judging by the coryneform morphology, peptidoglycans based upon 2,4-diaminobutyric acid, principal phospholipids phosphatidylglycerol and diphosphatidylglycerol, major menaquinone MK-11, predominant fatty acids of anteiso-C15:0, anteiso-C17:0, and iso-C16:0, and a DNA G + C base composition of 68.7 mol%, for which the name Leifsonia flava sp.
30047083	2	19	theme	Gram-positive	140:152	arg1	SYP-B2174T					106:115	SYP-B2174T	106:115	SYP-B2174T	106:115	SYP-B2174T is a yellow-pigmented, Gram-positive, non-motile, and rod-shaped actinobacterium isolated from the rhizospheric soil of Aquilegia viridiflora Pall.
30047083	2	19	theme	Gram-positive	140:152	arg1	actinobacterium					182:196	a yellow-pigmented, Gram-positive, non-motile, and rod-shaped actinobacterium	120:196	a yellow-pigmented, Gram-positive, non-motile, and rod-shaped actinobacterium isolated from the rhizospheric soil of Aquilegia viridiflora Pall	120:262	SYP-B2174T is a yellow-pigmented, Gram-positive, non-motile, and rod-shaped actinobacterium isolated from the rhizospheric soil of Aquilegia viridiflora Pall.
30047083	5	20	theme	rRNA	547:550	arg1	analysis					585:592	Comparative 16S rRNA gene sequence-based phylogenetic analysis	531:592	Comparative 16S rRNA gene sequence-based phylogenetic analysis	531:592	Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species Leifsonia kafniensis JCM 17021T and Leifsonia psychrotolerans DSM 22824T with similarities of 97.8 and 97.6%, respectively.
30047083	4	21	theme	optimal	390:396	arg1	growth					398:403	an optimal growth	387:403	an optimal growth being observed at 28°C. Growth occurs from 0 to 5% NaCl and at pH 6-8, with optimal growth being observed in 1% NaCl at pH 7	387:528	The strain's growth temperature ranges from 1 to 35°C, with an optimal growth being observed at 28°C. Growth occurs from 0 to 5% NaCl and at pH 6-8, with optimal growth being observed in 1% NaCl at pH 7.
30047083	9	22	theme	CGMCC	1361:1365	arg1	39963T					1397:1402	= CGMCC 1.15856T = DSM 105144T = KCTC 39963T	1359:1402	= CGMCC 1.15856T = DSM 105144T = KCTC 39963T	1359:1402	The type strain is SYP-B2174T (= CGMCC 1.15856T = DSM 105144T = KCTC 39963T).
30047083	9	22	theme	CGMCC	1361:1365	arg1	SYP-B2174T					1347:1356	SYP-B2174T	1347:1356	SYP-B2174T (= CGMCC 1.15856T = DSM 105144T = KCTC 39963T)	1347:1403	The type strain is SYP-B2174T (= CGMCC 1.15856T = DSM 105144T = KCTC 39963T).
30047083	5	23	with	clade	617:621	arg1	species					632:638	the species Leifsonia kafniensis JCM 17021T and Leifsonia psychrotolerans DSM 22824T	628:711	the species Leifsonia kafniensis JCM 17021T and Leifsonia psychrotolerans DSM 22824T	628:711	Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species Leifsonia kafniensis JCM 17021T and Leifsonia psychrotolerans DSM 22824T with similarities of 97.8 and 97.6%, respectively.
30047083	5	23	with	clade	617:621	arg1	22824T					706:711	Leifsonia psychrotolerans DSM 22824T	676:711	Leifsonia psychrotolerans DSM 22824T	676:711	Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species Leifsonia kafniensis JCM 17021T and Leifsonia psychrotolerans DSM 22824T with similarities of 97.8 and 97.6%, respectively.
30047083	5	23	with	clade	617:621	arg1	17021T					665:670	Leifsonia kafniensis JCM 17021T	640:670	Leifsonia kafniensis JCM 17021T	640:670	Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species Leifsonia kafniensis JCM 17021T and Leifsonia psychrotolerans DSM 22824T with similarities of 97.8 and 97.6%, respectively.
30047083	5	24	theme	Leifsonia	676:684	arg1	species					632:638	the species Leifsonia kafniensis JCM 17021T and Leifsonia psychrotolerans DSM 22824T	628:711	the species Leifsonia kafniensis JCM 17021T and Leifsonia psychrotolerans DSM 22824T	628:711	Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species Leifsonia kafniensis JCM 17021T and Leifsonia psychrotolerans DSM 22824T with similarities of 97.8 and 97.6%, respectively.
30047083	5	24	theme	Leifsonia	676:684	arg1	22824T					706:711	Leifsonia psychrotolerans DSM 22824T	676:711	Leifsonia psychrotolerans DSM 22824T	676:711	Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species Leifsonia kafniensis JCM 17021T and Leifsonia psychrotolerans DSM 22824T with similarities of 97.8 and 97.6%, respectively.
30047083	5	25	theme	gene	552:555	arg1	analysis					585:592	Comparative 16S rRNA gene sequence-based phylogenetic analysis	531:592	Comparative 16S rRNA gene sequence-based phylogenetic analysis	531:592	Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species Leifsonia kafniensis JCM 17021T and Leifsonia psychrotolerans DSM 22824T with similarities of 97.8 and 97.6%, respectively.
30047083	5	26	theme	%	747:747	arg1	similarities					718:729	similarities	718:729	similarities of 97.8 and 97.6%, respectively	718:761	Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species Leifsonia kafniensis JCM 17021T and Leifsonia psychrotolerans DSM 22824T with similarities of 97.8 and 97.6%, respectively.
30047083	7	27	theme	Leifsonia	955:963	arg1	strain					901:906	The strain	897:906	The strain	897:906	The strain was identified as a novel species of the genus Leifsonia judging by the coryneform morphology, peptidoglycans based upon 2,4-diaminobutyric acid, principal phospholipids phosphatidylglycerol and diphosphatidylglycerol, major menaquinone MK-11, predominant fatty acids of anteiso-C15:0, anteiso-C17:0, and iso-C16:0, and a DNA G + C base composition of 68.7 mol%, for which the name Leifsonia flava sp.
30047083	7	27	theme	Leifsonia	955:963	arg1	species					934:940	a novel species	926:940	a novel species of the genus Leifsonia judging by the coryneform morphology, peptidoglycans based upon 2,4-diaminobutyric acid, principal phospholipids phosphatidylglycerol and diphosphatidylglycerol, major menaquinone MK-11, predominant fatty acids of anteiso-C15:0, anteiso-C17:0, and iso-C16:0, and a DNA G + C base composition of 68.7 mol%, for which the name Leifsonia flava sp	926:1307	The strain was identified as a novel species of the genus Leifsonia judging by the coryneform morphology, peptidoglycans based upon 2,4-diaminobutyric acid, principal phospholipids phosphatidylglycerol and diphosphatidylglycerol, major menaquinone MK-11, predominant fatty acids of anteiso-C15:0, anteiso-C17:0, and iso-C16:0, and a DNA G + C base composition of 68.7 mol%, for which the name Leifsonia flava sp.
30047083	0	28	theme	flava	10:14	arg1	sp					16:17	Leifsonia flava sp	0:17	Leifsonia flava sp.	0:18	Leifsonia flava sp.
30047083	2	29	theme	yellow-pigmented	122:137	arg1	SYP-B2174T					106:115	SYP-B2174T	106:115	SYP-B2174T	106:115	SYP-B2174T is a yellow-pigmented, Gram-positive, non-motile, and rod-shaped actinobacterium isolated from the rhizospheric soil of Aquilegia viridiflora Pall.
30047083	2	29	theme	yellow-pigmented	122:137	arg1	actinobacterium					182:196	a yellow-pigmented, Gram-positive, non-motile, and rod-shaped actinobacterium	120:196	a yellow-pigmented, Gram-positive, non-motile, and rod-shaped actinobacterium isolated from the rhizospheric soil of Aquilegia viridiflora Pall	120:262	SYP-B2174T is a yellow-pigmented, Gram-positive, non-motile, and rod-shaped actinobacterium isolated from the rhizospheric soil of Aquilegia viridiflora Pall.
30047083	5	30	theme	sequence-based	557:570	arg1	analysis					585:592	Comparative 16S rRNA gene sequence-based phylogenetic analysis	531:592	Comparative 16S rRNA gene sequence-based phylogenetic analysis	531:592	Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species Leifsonia kafniensis JCM 17021T and Leifsonia psychrotolerans DSM 22824T with similarities of 97.8 and 97.6%, respectively.
30047083	4	31	dep	NaCl	456:459	arg1	%					454:454	%	454:454	%	454:454	The strain's growth temperature ranges from 1 to 35°C, with an optimal growth being observed at 28°C. Growth occurs from 0 to 5% NaCl and at pH 6-8, with optimal growth being observed in 1% NaCl at pH 7.
30047083	9	32	theme	105144T	1382:1388	arg1	39963T					1397:1402	= CGMCC 1.15856T = DSM 105144T = KCTC 39963T	1359:1402	= CGMCC 1.15856T = DSM 105144T = KCTC 39963T	1359:1402	The type strain is SYP-B2174T (= CGMCC 1.15856T = DSM 105144T = KCTC 39963T).
30047083	9	32	theme	105144T	1382:1388	arg1	SYP-B2174T					1347:1356	SYP-B2174T	1347:1356	SYP-B2174T (= CGMCC 1.15856T = DSM 105144T = KCTC 39963T)	1347:1403	The type strain is SYP-B2174T (= CGMCC 1.15856T = DSM 105144T = KCTC 39963T).
30047083	7	33	theme	mol	1265:1267	arg1	%					1268:1268	68.7 mol%	1260:1268	68.7 mol%	1260:1268	The strain was identified as a novel species of the genus Leifsonia judging by the coryneform morphology, peptidoglycans based upon 2,4-diaminobutyric acid, principal phospholipids phosphatidylglycerol and diphosphatidylglycerol, major menaquinone MK-11, predominant fatty acids of anteiso-C15:0, anteiso-C17:0, and iso-C16:0, and a DNA G + C base composition of 68.7 mol%, for which the name Leifsonia flava sp.
30047083	0	34	theme	Leifsonia	0:8	arg1	sp					16:17	Leifsonia flava sp	0:17	Leifsonia flava sp.	0:18	Leifsonia flava sp.
30047083	1	35	attach	isolated	50:57	arg2	actinobacterium					34:48	a novel actinobacterium	26:48	a novel actinobacterium isolated from the rhizosphere of Aquilegia viridiflora	26:103	nov., a novel actinobacterium isolated from the rhizosphere of Aquilegia viridiflora.
30047083	1	35	attach	isolated	50:57	arg1	rhizosphere					68:78	the rhizosphere	64:78	the rhizosphere of Aquilegia viridiflora	64:103	nov., a novel actinobacterium isolated from the rhizosphere of Aquilegia viridiflora.
30047083	5	36	theme	phylogenetic	572:583	arg1	analysis					585:592	Comparative 16S rRNA gene sequence-based phylogenetic analysis	531:592	Comparative 16S rRNA gene sequence-based phylogenetic analysis	531:592	Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species Leifsonia kafniensis JCM 17021T and Leifsonia psychrotolerans DSM 22824T with similarities of 97.8 and 97.6%, respectively.
30047083	7	37	theme	2,4-diaminobutyric	1029:1046	arg1	acid					1048:1051	2,4-diaminobutyric acid	1029:1051	2,4-diaminobutyric acid	1029:1051	The strain was identified as a novel species of the genus Leifsonia judging by the coryneform morphology, peptidoglycans based upon 2,4-diaminobutyric acid, principal phospholipids phosphatidylglycerol and diphosphatidylglycerol, major menaquinone MK-11, predominant fatty acids of anteiso-C15:0, anteiso-C17:0, and iso-C16:0, and a DNA G + C base composition of 68.7 mol%, for which the name Leifsonia flava sp.
30047083	2	38	attach	isolated	198:205	arg2	actinobacterium					182:196	a yellow-pigmented, Gram-positive, non-motile, and rod-shaped actinobacterium	120:196	a yellow-pigmented, Gram-positive, non-motile, and rod-shaped actinobacterium isolated from the rhizospheric soil of Aquilegia viridiflora Pall	120:262	SYP-B2174T is a yellow-pigmented, Gram-positive, non-motile, and rod-shaped actinobacterium isolated from the rhizospheric soil of Aquilegia viridiflora Pall.
30047083	2	38	attach	isolated	198:205	arg2	SYP-B2174T					106:115	SYP-B2174T	106:115	SYP-B2174T	106:115	SYP-B2174T is a yellow-pigmented, Gram-positive, non-motile, and rod-shaped actinobacterium isolated from the rhizospheric soil of Aquilegia viridiflora Pall.
30047083	2	38	attach	isolated	198:205	arg1	soil					229:232	the rhizospheric soil	212:232	the rhizospheric soil of Aquilegia viridiflora Pall	212:262	SYP-B2174T is a yellow-pigmented, Gram-positive, non-motile, and rod-shaped actinobacterium isolated from the rhizospheric soil of Aquilegia viridiflora Pall.
30047083	7	39	theme	menaquinone	1133:1143	arg1	MK-11					1145:1149	major menaquinone MK-11	1127:1149	major menaquinone MK-11	1127:1149	The strain was identified as a novel species of the genus Leifsonia judging by the coryneform morphology, peptidoglycans based upon 2,4-diaminobutyric acid, principal phospholipids phosphatidylglycerol and diphosphatidylglycerol, major menaquinone MK-11, predominant fatty acids of anteiso-C15:0, anteiso-C17:0, and iso-C16:0, and a DNA G + C base composition of 68.7 mol%, for which the name Leifsonia flava sp.
30047083	7	39	theme	menaquinone	1133:1143	arg1	diphosphatidylglycerol					1103:1124	diphosphatidylglycerol	1103:1124	diphosphatidylglycerol	1103:1124	The strain was identified as a novel species of the genus Leifsonia judging by the coryneform morphology, peptidoglycans based upon 2,4-diaminobutyric acid, principal phospholipids phosphatidylglycerol and diphosphatidylglycerol, major menaquinone MK-11, predominant fatty acids of anteiso-C15:0, anteiso-C17:0, and iso-C16:0, and a DNA G + C base composition of 68.7 mol%, for which the name Leifsonia flava sp.
30047083	4	40	dep	%	454:454	arg1	5					453:453	5	453:453	5	453:453	The strain's growth temperature ranges from 1 to 35°C, with an optimal growth being observed at 28°C. Growth occurs from 0 to 5% NaCl and at pH 6-8, with optimal growth being observed in 1% NaCl at pH 7.
30047083	3	41	theme	Xinjiang	284:291	arg1	region					310:315	the Xinjiang uygur autonomous region	280:315	the Xinjiang uygur autonomous region of China	280:324	collected from the Xinjiang uygur autonomous region of China.
30047083	5	42	theme	16S	543:545	arg1	analysis					585:592	Comparative 16S rRNA gene sequence-based phylogenetic analysis	531:592	Comparative 16S rRNA gene sequence-based phylogenetic analysis	531:592	Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species Leifsonia kafniensis JCM 17021T and Leifsonia psychrotolerans DSM 22824T with similarities of 97.8 and 97.6%, respectively.
30047083	1	43	theme	Aquilegia	83:91	arg1	viridiflora					93:103	Aquilegia viridiflora	83:103	Aquilegia viridiflora	83:103	nov., a novel actinobacterium isolated from the rhizosphere of Aquilegia viridiflora.
30047083	7	44	theme	genus	949:953	arg1	Leifsonia					955:963	the genus Leifsonia	945:963	the genus Leifsonia judging by the coryneform morphology, peptidoglycans based upon 2,4-diaminobutyric acid, principal phospholipids phosphatidylglycerol and diphosphatidylglycerol, major menaquinone MK-11, predominant fatty acids of anteiso-C15:0, anteiso-C17:0, and iso-C16:0, and a DNA G + C base composition of 68.7 mol%, for which the name Leifsonia flava sp	945:1307	The strain was identified as a novel species of the genus Leifsonia judging by the coryneform morphology, peptidoglycans based upon 2,4-diaminobutyric acid, principal phospholipids phosphatidylglycerol and diphosphatidylglycerol, major menaquinone MK-11, predominant fatty acids of anteiso-C15:0, anteiso-C17:0, and iso-C16:0, and a DNA G + C base composition of 68.7 mol%, for which the name Leifsonia flava sp.
30047083	4	45	theme	1	514:514	arg1	%					515:515	%	515:515	%	515:515	The strain's growth temperature ranges from 1 to 35°C, with an optimal growth being observed at 28°C. Growth occurs from 0 to 5% NaCl and at pH 6-8, with optimal growth being observed in 1% NaCl at pH 7.
30047083	2	46	theme	rod-shaped	171:180	arg1	SYP-B2174T					106:115	SYP-B2174T	106:115	SYP-B2174T	106:115	SYP-B2174T is a yellow-pigmented, Gram-positive, non-motile, and rod-shaped actinobacterium isolated from the rhizospheric soil of Aquilegia viridiflora Pall.
30047083	2	46	theme	rod-shaped	171:180	arg1	actinobacterium					182:196	a yellow-pigmented, Gram-positive, non-motile, and rod-shaped actinobacterium	120:196	a yellow-pigmented, Gram-positive, non-motile, and rod-shaped actinobacterium isolated from the rhizospheric soil of Aquilegia viridiflora Pall	120:262	SYP-B2174T is a yellow-pigmented, Gram-positive, non-motile, and rod-shaped actinobacterium isolated from the rhizospheric soil of Aquilegia viridiflora Pall.
30047083	1	47	theme	viridiflora	93:103	arg1	rhizosphere					68:78	the rhizosphere	64:78	the rhizosphere of Aquilegia viridiflora	64:103	nov., a novel actinobacterium isolated from the rhizosphere of Aquilegia viridiflora.
30047083	9	48	theme	DSM	1378:1380	arg1	39963T					1397:1402	= CGMCC 1.15856T = DSM 105144T = KCTC 39963T	1359:1402	= CGMCC 1.15856T = DSM 105144T = KCTC 39963T	1359:1402	The type strain is SYP-B2174T (= CGMCC 1.15856T = DSM 105144T = KCTC 39963T).
30047083	9	48	theme	DSM	1378:1380	arg1	SYP-B2174T					1347:1356	SYP-B2174T	1347:1356	SYP-B2174T (= CGMCC 1.15856T = DSM 105144T = KCTC 39963T)	1347:1403	The type strain is SYP-B2174T (= CGMCC 1.15856T = DSM 105144T = KCTC 39963T).
30047083	5	49	theme	Leifsonia	640:648	arg1	species					632:638	the species Leifsonia kafniensis JCM 17021T and Leifsonia psychrotolerans DSM 22824T	628:711	the species Leifsonia kafniensis JCM 17021T and Leifsonia psychrotolerans DSM 22824T	628:711	Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species Leifsonia kafniensis JCM 17021T and Leifsonia psychrotolerans DSM 22824T with similarities of 97.8 and 97.6%, respectively.
30047083	5	49	theme	Leifsonia	640:648	arg1	17021T					665:670	Leifsonia kafniensis JCM 17021T	640:670	Leifsonia kafniensis JCM 17021T	640:670	Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species Leifsonia kafniensis JCM 17021T and Leifsonia psychrotolerans DSM 22824T with similarities of 97.8 and 97.6%, respectively.
30047083	7	50	theme	predominant	1152:1162	arg1	acids					1170:1174	predominant fatty acids	1152:1174	predominant fatty acids	1152:1174	The strain was identified as a novel species of the genus Leifsonia judging by the coryneform morphology, peptidoglycans based upon 2,4-diaminobutyric acid, principal phospholipids phosphatidylglycerol and diphosphatidylglycerol, major menaquinone MK-11, predominant fatty acids of anteiso-C15:0, anteiso-C17:0, and iso-C16:0, and a DNA G + C base composition of 68.7 mol%, for which the name Leifsonia flava sp.
30047083	7	50	theme	predominant	1152:1162	arg1	diphosphatidylglycerol					1103:1124	diphosphatidylglycerol	1103:1124	diphosphatidylglycerol	1103:1124	The strain was identified as a novel species of the genus Leifsonia judging by the coryneform morphology, peptidoglycans based upon 2,4-diaminobutyric acid, principal phospholipids phosphatidylglycerol and diphosphatidylglycerol, major menaquinone MK-11, predominant fatty acids of anteiso-C15:0, anteiso-C17:0, and iso-C16:0, and a DNA G + C base composition of 68.7 mol%, for which the name Leifsonia flava sp.
30047083	2	51	theme	Aquilegia	237:245	arg1	Pall					259:262	Aquilegia viridiflora Pall	237:262	Aquilegia viridiflora Pall	237:262	SYP-B2174T is a yellow-pigmented, Gram-positive, non-motile, and rod-shaped actinobacterium isolated from the rhizospheric soil of Aquilegia viridiflora Pall.
30047083	7	52	theme	%	1268:1268	arg1	phospholipids					1064:1076	principal phospholipids	1054:1076	principal phospholipids phosphatidylglycerol and diphosphatidylglycerol, major menaquinone MK-11, predominant fatty acids of anteiso-C15:0, anteiso-C17:0, and iso-C16:0	1054:1221	The strain was identified as a novel species of the genus Leifsonia judging by the coryneform morphology, peptidoglycans based upon 2,4-diaminobutyric acid, principal phospholipids phosphatidylglycerol and diphosphatidylglycerol, major menaquinone MK-11, predominant fatty acids of anteiso-C15:0, anteiso-C17:0, and iso-C16:0, and a DNA G + C base composition of 68.7 mol%, for which the name Leifsonia flava sp.
30047083	7	52	theme	%	1268:1268	arg1	phosphatidylglycerol					1078:1097	phosphatidylglycerol	1078:1097	phosphatidylglycerol	1078:1097	The strain was identified as a novel species of the genus Leifsonia judging by the coryneform morphology, peptidoglycans based upon 2,4-diaminobutyric acid, principal phospholipids phosphatidylglycerol and diphosphatidylglycerol, major menaquinone MK-11, predominant fatty acids of anteiso-C15:0, anteiso-C17:0, and iso-C16:0, and a DNA G + C base composition of 68.7 mol%, for which the name Leifsonia flava sp.
30047083	7	52	theme	%	1268:1268	arg1	composition					1245:1255	a DNA G + C base composition	1228:1255	composition	1245:1255	The strain was identified as a novel species of the genus Leifsonia judging by the coryneform morphology, peptidoglycans based upon 2,4-diaminobutyric acid, principal phospholipids phosphatidylglycerol and diphosphatidylglycerol, major menaquinone MK-11, predominant fatty acids of anteiso-C15:0, anteiso-C17:0, and iso-C16:0, and a DNA G + C base composition of 68.7 mol%, for which the name Leifsonia flava sp.
30047083	7	52	theme	%	1268:1268	arg1	diphosphatidylglycerol					1103:1124	diphosphatidylglycerol	1103:1124	diphosphatidylglycerol	1103:1124	The strain was identified as a novel species of the genus Leifsonia judging by the coryneform morphology, peptidoglycans based upon 2,4-diaminobutyric acid, principal phospholipids phosphatidylglycerol and diphosphatidylglycerol, major menaquinone MK-11, predominant fatty acids of anteiso-C15:0, anteiso-C17:0, and iso-C16:0, and a DNA G + C base composition of 68.7 mol%, for which the name Leifsonia flava sp.
30047083	7	52	theme	%	1268:1268	arg1	G					1234:1234	a DNA G + C base composition	1228:1255	G	1234:1234	The strain was identified as a novel species of the genus Leifsonia judging by the coryneform morphology, peptidoglycans based upon 2,4-diaminobutyric acid, principal phospholipids phosphatidylglycerol and diphosphatidylglycerol, major menaquinone MK-11, predominant fatty acids of anteiso-C15:0, anteiso-C17:0, and iso-C16:0, and a DNA G + C base composition of 68.7 mol%, for which the name Leifsonia flava sp.
30047083	7	52	theme	%	1268:1268	arg1	morphology					991:1000	the coryneform morphology	976:1000	the coryneform morphology	976:1000	The strain was identified as a novel species of the genus Leifsonia judging by the coryneform morphology, peptidoglycans based upon 2,4-diaminobutyric acid, principal phospholipids phosphatidylglycerol and diphosphatidylglycerol, major menaquinone MK-11, predominant fatty acids of anteiso-C15:0, anteiso-C17:0, and iso-C16:0, and a DNA G + C base composition of 68.7 mol%, for which the name Leifsonia flava sp.
30047083	7	52	theme	%	1268:1268	arg1	peptidoglycans					1003:1016	peptidoglycans	1003:1016	peptidoglycans based upon 2,4-diaminobutyric acid	1003:1051	The strain was identified as a novel species of the genus Leifsonia judging by the coryneform morphology, peptidoglycans based upon 2,4-diaminobutyric acid, principal phospholipids phosphatidylglycerol and diphosphatidylglycerol, major menaquinone MK-11, predominant fatty acids of anteiso-C15:0, anteiso-C17:0, and iso-C16:0, and a DNA G + C base composition of 68.7 mol%, for which the name Leifsonia flava sp.
30047083	7	53	theme	base	1240:1243	arg1	composition					1245:1255	a DNA G + C base composition	1228:1255	composition	1245:1255	The strain was identified as a novel species of the genus Leifsonia judging by the coryneform morphology, peptidoglycans based upon 2,4-diaminobutyric acid, principal phospholipids phosphatidylglycerol and diphosphatidylglycerol, major menaquinone MK-11, predominant fatty acids of anteiso-C15:0, anteiso-C17:0, and iso-C16:0, and a DNA G + C base composition of 68.7 mol%, for which the name Leifsonia flava sp.
30047083	7	54	theme	major	1127:1131	arg1	MK-11					1145:1149	major menaquinone MK-11	1127:1149	major menaquinone MK-11	1127:1149	The strain was identified as a novel species of the genus Leifsonia judging by the coryneform morphology, peptidoglycans based upon 2,4-diaminobutyric acid, principal phospholipids phosphatidylglycerol and diphosphatidylglycerol, major menaquinone MK-11, predominant fatty acids of anteiso-C15:0, anteiso-C17:0, and iso-C16:0, and a DNA G + C base composition of 68.7 mol%, for which the name Leifsonia flava sp.
30047083	7	54	theme	major	1127:1131	arg1	diphosphatidylglycerol					1103:1124	diphosphatidylglycerol	1103:1124	diphosphatidylglycerol	1103:1124	The strain was identified as a novel species of the genus Leifsonia judging by the coryneform morphology, peptidoglycans based upon 2,4-diaminobutyric acid, principal phospholipids phosphatidylglycerol and diphosphatidylglycerol, major menaquinone MK-11, predominant fatty acids of anteiso-C15:0, anteiso-C17:0, and iso-C16:0, and a DNA G + C base composition of 68.7 mol%, for which the name Leifsonia flava sp.
30047083	5	55	theme	kafniensis	650:659	arg1	species					632:638	the species Leifsonia kafniensis JCM 17021T and Leifsonia psychrotolerans DSM 22824T	628:711	the species Leifsonia kafniensis JCM 17021T and Leifsonia psychrotolerans DSM 22824T	628:711	Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species Leifsonia kafniensis JCM 17021T and Leifsonia psychrotolerans DSM 22824T with similarities of 97.8 and 97.6%, respectively.
30047083	5	55	theme	kafniensis	650:659	arg1	17021T					665:670	Leifsonia kafniensis JCM 17021T	640:670	Leifsonia kafniensis JCM 17021T	640:670	Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species Leifsonia kafniensis JCM 17021T and Leifsonia psychrotolerans DSM 22824T with similarities of 97.8 and 97.6%, respectively.
30047083	7	56	dep	phospholipids	1064:1076	arg1	MK-11					1145:1149	major menaquinone MK-11	1127:1149	major menaquinone MK-11	1127:1149	The strain was identified as a novel species of the genus Leifsonia judging by the coryneform morphology, peptidoglycans based upon 2,4-diaminobutyric acid, principal phospholipids phosphatidylglycerol and diphosphatidylglycerol, major menaquinone MK-11, predominant fatty acids of anteiso-C15:0, anteiso-C17:0, and iso-C16:0, and a DNA G + C base composition of 68.7 mol%, for which the name Leifsonia flava sp.
30047083	7	56	dep	phospholipids	1064:1076	arg1	phospholipids					1064:1076	principal phospholipids	1054:1076	principal phospholipids phosphatidylglycerol and diphosphatidylglycerol, major menaquinone MK-11, predominant fatty acids of anteiso-C15:0, anteiso-C17:0, and iso-C16:0	1054:1221	The strain was identified as a novel species of the genus Leifsonia judging by the coryneform morphology, peptidoglycans based upon 2,4-diaminobutyric acid, principal phospholipids phosphatidylglycerol and diphosphatidylglycerol, major menaquinone MK-11, predominant fatty acids of anteiso-C15:0, anteiso-C17:0, and iso-C16:0, and a DNA G + C base composition of 68.7 mol%, for which the name Leifsonia flava sp.
30047083	7	56	dep	phospholipids	1064:1076	arg1	diphosphatidylglycerol					1103:1124	diphosphatidylglycerol	1103:1124	diphosphatidylglycerol	1103:1124	The strain was identified as a novel species of the genus Leifsonia judging by the coryneform morphology, peptidoglycans based upon 2,4-diaminobutyric acid, principal phospholipids phosphatidylglycerol and diphosphatidylglycerol, major menaquinone MK-11, predominant fatty acids of anteiso-C15:0, anteiso-C17:0, and iso-C16:0, and a DNA G + C base composition of 68.7 mol%, for which the name Leifsonia flava sp.
30047083	7	56	dep	phospholipids	1064:1076	arg1	acids					1170:1174	predominant fatty acids	1152:1174	predominant fatty acids	1152:1174	The strain was identified as a novel species of the genus Leifsonia judging by the coryneform morphology, peptidoglycans based upon 2,4-diaminobutyric acid, principal phospholipids phosphatidylglycerol and diphosphatidylglycerol, major menaquinone MK-11, predominant fatty acids of anteiso-C15:0, anteiso-C17:0, and iso-C16:0, and a DNA G + C base composition of 68.7 mol%, for which the name Leifsonia flava sp.
30047083	7	56	dep	phospholipids	1064:1076	arg1	phosphatidylglycerol					1078:1097	phosphatidylglycerol	1078:1097	phosphatidylglycerol	1078:1097	The strain was identified as a novel species of the genus Leifsonia judging by the coryneform morphology, peptidoglycans based upon 2,4-diaminobutyric acid, principal phospholipids phosphatidylglycerol and diphosphatidylglycerol, major menaquinone MK-11, predominant fatty acids of anteiso-C15:0, anteiso-C17:0, and iso-C16:0, and a DNA G + C base composition of 68.7 mol%, for which the name Leifsonia flava sp.
30047083	4	57	theme	optimal	481:487	arg1	growth					489:494	optimal growth	481:494	optimal growth	481:494	The strain's growth temperature ranges from 1 to 35°C, with an optimal growth being observed at 28°C. Growth occurs from 0 to 5% NaCl and at pH 6-8, with optimal growth being observed in 1% NaCl at pH 7.
30047083	5	58	theme	97.8	734:737	arg1	similarities					718:729	similarities	718:729	similarities of 97.8 and 97.6%, respectively	718:761	Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species Leifsonia kafniensis JCM 17021T and Leifsonia psychrotolerans DSM 22824T with similarities of 97.8 and 97.6%, respectively.
30047083	5	59	theme	JCM	661:663	arg1	species					632:638	the species Leifsonia kafniensis JCM 17021T and Leifsonia psychrotolerans DSM 22824T	628:711	the species Leifsonia kafniensis JCM 17021T and Leifsonia psychrotolerans DSM 22824T	628:711	Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species Leifsonia kafniensis JCM 17021T and Leifsonia psychrotolerans DSM 22824T with similarities of 97.8 and 97.6%, respectively.
30047083	5	59	theme	JCM	661:663	arg1	17021T					665:670	Leifsonia kafniensis JCM 17021T	640:670	Leifsonia kafniensis JCM 17021T	640:670	Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species Leifsonia kafniensis JCM 17021T and Leifsonia psychrotolerans DSM 22824T with similarities of 97.8 and 97.6%, respectively.
30047083	2	60	theme	non-motile	155:164	arg1	SYP-B2174T					106:115	SYP-B2174T	106:115	SYP-B2174T	106:115	SYP-B2174T is a yellow-pigmented, Gram-positive, non-motile, and rod-shaped actinobacterium isolated from the rhizospheric soil of Aquilegia viridiflora Pall.
30047083	2	60	theme	non-motile	155:164	arg1	actinobacterium					182:196	a yellow-pigmented, Gram-positive, non-motile, and rod-shaped actinobacterium	120:196	a yellow-pigmented, Gram-positive, non-motile, and rod-shaped actinobacterium isolated from the rhizospheric soil of Aquilegia viridiflora Pall	120:262	SYP-B2174T is a yellow-pigmented, Gram-positive, non-motile, and rod-shaped actinobacterium isolated from the rhizospheric soil of Aquilegia viridiflora Pall.
30047083	6	61	theme	strain	804:809	arg1	SYP-B2174T					811:820	the strain SYP-B2174T	800:820	the strain SYP-B2174T to its closest phylogenetic neighbors	800:858	The DNA-DNA hybridization values of the strain SYP-B2174T to its closest phylogenetic neighbors were significantly lower than 35.7%.
30047083	4	62	theme	growth	340:345	arg1	temperature					347:357	The strain's growth temperature	327:357	The strain's growth temperature	327:357	The strain's growth temperature ranges from 1 to 35°C, with an optimal growth being observed at 28°C. Growth occurs from 0 to 5% NaCl and at pH 6-8, with optimal growth being observed in 1% NaCl at pH 7.
30047083	9	63	theme	KCTC	1392:1395	arg1	39963T					1397:1402	= CGMCC 1.15856T = DSM 105144T = KCTC 39963T	1359:1402	= CGMCC 1.15856T = DSM 105144T = KCTC 39963T	1359:1402	The type strain is SYP-B2174T (= CGMCC 1.15856T = DSM 105144T = KCTC 39963T).
30047083	9	63	theme	KCTC	1392:1395	arg1	SYP-B2174T					1347:1356	SYP-B2174T	1347:1356	SYP-B2174T (= CGMCC 1.15856T = DSM 105144T = KCTC 39963T)	1347:1403	The type strain is SYP-B2174T (= CGMCC 1.15856T = DSM 105144T = KCTC 39963T).
30047083	3	64	theme	China	320:324	arg1	region					310:315	the Xinjiang uygur autonomous region	280:315	the Xinjiang uygur autonomous region of China	280:324	collected from the Xinjiang uygur autonomous region of China.
30047083	6	65	theme	hybridization	776:788	arg1	lower					879:883	lower	879:883	lower	879:883	The DNA-DNA hybridization values of the strain SYP-B2174T to its closest phylogenetic neighbors were significantly lower than 35.7%.
30047083	6	65	theme	hybridization	776:788	arg1	values					790:795	The DNA-DNA hybridization values	764:795	The DNA-DNA hybridization values of the strain SYP-B2174T to its closest phylogenetic neighbors	764:858	The DNA-DNA hybridization values of the strain SYP-B2174T to its closest phylogenetic neighbors were significantly lower than 35.7%.
30047083	2	66	theme	rhizospheric	216:227	arg1	soil					229:232	the rhizospheric soil	212:232	the rhizospheric soil of Aquilegia viridiflora Pall	212:262	SYP-B2174T is a yellow-pigmented, Gram-positive, non-motile, and rod-shaped actinobacterium isolated from the rhizospheric soil of Aquilegia viridiflora Pall.
30047083	7	67	theme	C	1238:1238	arg1	composition					1245:1255	a DNA G + C base composition	1228:1255	composition	1245:1255	The strain was identified as a novel species of the genus Leifsonia judging by the coryneform morphology, peptidoglycans based upon 2,4-diaminobutyric acid, principal phospholipids phosphatidylglycerol and diphosphatidylglycerol, major menaquinone MK-11, predominant fatty acids of anteiso-C15:0, anteiso-C17:0, and iso-C16:0, and a DNA G + C base composition of 68.7 mol%, for which the name Leifsonia flava sp.
30047083	1	68	theme	novel	28:32	arg1	actinobacterium					34:48	a novel actinobacterium	26:48	a novel actinobacterium isolated from the rhizosphere of Aquilegia viridiflora	26:103	nov., a novel actinobacterium isolated from the rhizosphere of Aquilegia viridiflora.
30047083	3	69	theme	autonomous	299:308	arg1	region					310:315	the Xinjiang uygur autonomous region	280:315	the Xinjiang uygur autonomous region of China	280:324	collected from the Xinjiang uygur autonomous region of China.
30047083	6	70	theme	DNA-DNA	768:774	arg1	hybridization					776:788	DNA-DNA hybridization	768:788	The DNA-DNA hybridization values of the strain SYP-B2174T to its closest phylogenetic neighbors	764:858	The DNA-DNA hybridization values of the strain SYP-B2174T to its closest phylogenetic neighbors were significantly lower than 35.7%.
30047083	7	71	theme	name	1285:1288	arg1	sp					1306:1307	the name Leifsonia flava sp	1281:1307	the name Leifsonia flava sp	1281:1307	The strain was identified as a novel species of the genus Leifsonia judging by the coryneform morphology, peptidoglycans based upon 2,4-diaminobutyric acid, principal phospholipids phosphatidylglycerol and diphosphatidylglycerol, major menaquinone MK-11, predominant fatty acids of anteiso-C15:0, anteiso-C17:0, and iso-C16:0, and a DNA G + C base composition of 68.7 mol%, for which the name Leifsonia flava sp.
30047083	1	72	dep	actinobacterium	34:48	arg1	nov.					20:23	nov.	20:23	nov.	20:23	nov., a novel actinobacterium isolated from the rhizosphere of Aquilegia viridiflora.
30047083	4	73	dep	35°C	376:379	arg1	to					373:374	to	373:374	to	373:374	The strain's growth temperature ranges from 1 to 35°C, with an optimal growth being observed at 28°C. Growth occurs from 0 to 5% NaCl and at pH 6-8, with optimal growth being observed in 1% NaCl at pH 7.
30047083	4	74	theme	28°C.	423:427	arg1	Growth					429:434	28°C. Growth	423:434	28°C. Growth	423:434	The strain's growth temperature ranges from 1 to 35°C, with an optimal growth being observed at 28°C. Growth occurs from 0 to 5% NaCl and at pH 6-8, with optimal growth being observed in 1% NaCl at pH 7.
30047083	7	75	theme	Leifsonia	1290:1298	arg1	sp					1306:1307	the name Leifsonia flava sp	1281:1307	the name Leifsonia flava sp	1281:1307	The strain was identified as a novel species of the genus Leifsonia judging by the coryneform morphology, peptidoglycans based upon 2,4-diaminobutyric acid, principal phospholipids phosphatidylglycerol and diphosphatidylglycerol, major menaquinone MK-11, predominant fatty acids of anteiso-C15:0, anteiso-C17:0, and iso-C16:0, and a DNA G + C base composition of 68.7 mol%, for which the name Leifsonia flava sp.
30047083	7	76	theme	DNA	1230:1232	arg1	G					1234:1234	a DNA G + C base composition	1228:1255	G	1234:1234	The strain was identified as a novel species of the genus Leifsonia judging by the coryneform morphology, peptidoglycans based upon 2,4-diaminobutyric acid, principal phospholipids phosphatidylglycerol and diphosphatidylglycerol, major menaquinone MK-11, predominant fatty acids of anteiso-C15:0, anteiso-C17:0, and iso-C16:0, and a DNA G + C base composition of 68.7 mol%, for which the name Leifsonia flava sp.
30047083	5	77	theme	psychrotolerans	686:700	arg1	species					632:638	the species Leifsonia kafniensis JCM 17021T and Leifsonia psychrotolerans DSM 22824T	628:711	the species Leifsonia kafniensis JCM 17021T and Leifsonia psychrotolerans DSM 22824T	628:711	Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species Leifsonia kafniensis JCM 17021T and Leifsonia psychrotolerans DSM 22824T with similarities of 97.8 and 97.6%, respectively.
30047083	5	77	theme	psychrotolerans	686:700	arg1	22824T					706:711	Leifsonia psychrotolerans DSM 22824T	676:711	Leifsonia psychrotolerans DSM 22824T	676:711	Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species Leifsonia kafniensis JCM 17021T and Leifsonia psychrotolerans DSM 22824T with similarities of 97.8 and 97.6%, respectively.
30047083	9	78	theme	=	1390:1390	arg1	39963T					1397:1402	= CGMCC 1.15856T = DSM 105144T = KCTC 39963T	1359:1402	= CGMCC 1.15856T = DSM 105144T = KCTC 39963T	1359:1402	The type strain is SYP-B2174T (= CGMCC 1.15856T = DSM 105144T = KCTC 39963T).
30047083	9	78	theme	=	1390:1390	arg1	SYP-B2174T					1347:1356	SYP-B2174T	1347:1356	SYP-B2174T (= CGMCC 1.15856T = DSM 105144T = KCTC 39963T)	1347:1403	The type strain is SYP-B2174T (= CGMCC 1.15856T = DSM 105144T = KCTC 39963T).
30047083	2	79	theme	Pall	259:262	arg1	soil					229:232	the rhizospheric soil	212:232	the rhizospheric soil of Aquilegia viridiflora Pall	212:262	SYP-B2174T is a yellow-pigmented, Gram-positive, non-motile, and rod-shaped actinobacterium isolated from the rhizospheric soil of Aquilegia viridiflora Pall.
30047083	7	80	theme	novel	928:932	arg1	strain					901:906	The strain	897:906	The strain	897:906	The strain was identified as a novel species of the genus Leifsonia judging by the coryneform morphology, peptidoglycans based upon 2,4-diaminobutyric acid, principal phospholipids phosphatidylglycerol and diphosphatidylglycerol, major menaquinone MK-11, predominant fatty acids of anteiso-C15:0, anteiso-C17:0, and iso-C16:0, and a DNA G + C base composition of 68.7 mol%, for which the name Leifsonia flava sp.
30047083	7	80	theme	novel	928:932	arg1	species					934:940	a novel species	926:940	a novel species of the genus Leifsonia judging by the coryneform morphology, peptidoglycans based upon 2,4-diaminobutyric acid, principal phospholipids phosphatidylglycerol and diphosphatidylglycerol, major menaquinone MK-11, predominant fatty acids of anteiso-C15:0, anteiso-C17:0, and iso-C16:0, and a DNA G + C base composition of 68.7 mol%, for which the name Leifsonia flava sp	926:1307	The strain was identified as a novel species of the genus Leifsonia judging by the coryneform morphology, peptidoglycans based upon 2,4-diaminobutyric acid, principal phospholipids phosphatidylglycerol and diphosphatidylglycerol, major menaquinone MK-11, predominant fatty acids of anteiso-C15:0, anteiso-C17:0, and iso-C16:0, and a DNA G + C base composition of 68.7 mol%, for which the name Leifsonia flava sp.
30047083	9	81	theme	=	1359:1359	arg1	39963T					1397:1402	= CGMCC 1.15856T = DSM 105144T = KCTC 39963T	1359:1402	= CGMCC 1.15856T = DSM 105144T = KCTC 39963T	1359:1402	The type strain is SYP-B2174T (= CGMCC 1.15856T = DSM 105144T = KCTC 39963T).
30047083	9	81	theme	=	1359:1359	arg1	SYP-B2174T					1347:1356	SYP-B2174T	1347:1356	SYP-B2174T (= CGMCC 1.15856T = DSM 105144T = KCTC 39963T)	1347:1403	The type strain is SYP-B2174T (= CGMCC 1.15856T = DSM 105144T = KCTC 39963T).
30047083	5	82	theme	DSM	702:704	arg1	species					632:638	the species Leifsonia kafniensis JCM 17021T and Leifsonia psychrotolerans DSM 22824T	628:711	the species Leifsonia kafniensis JCM 17021T and Leifsonia psychrotolerans DSM 22824T	628:711	Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species Leifsonia kafniensis JCM 17021T and Leifsonia psychrotolerans DSM 22824T with similarities of 97.8 and 97.6%, respectively.
30047083	5	82	theme	DSM	702:704	arg1	22824T					706:711	Leifsonia psychrotolerans DSM 22824T	676:711	Leifsonia psychrotolerans DSM 22824T	676:711	Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species Leifsonia kafniensis JCM 17021T and Leifsonia psychrotolerans DSM 22824T with similarities of 97.8 and 97.6%, respectively.
30047083	2	83	theme	viridiflora	247:257	arg1	Pall					259:262	Aquilegia viridiflora Pall	237:262	Aquilegia viridiflora Pall	237:262	SYP-B2174T is a yellow-pigmented, Gram-positive, non-motile, and rod-shaped actinobacterium isolated from the rhizospheric soil of Aquilegia viridiflora Pall.
30047083	5	84	from	strain	605:610	arg1	clade					617:621	a clade	615:621	a clade with the species Leifsonia kafniensis JCM 17021T and Leifsonia psychrotolerans DSM 22824T	615:711	Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species Leifsonia kafniensis JCM 17021T and Leifsonia psychrotolerans DSM 22824T with similarities of 97.8 and 97.6%, respectively.
29532887	0	0	theme	lung	78:81	arg1	injury					83:88	acute lung injury	72:88	acute lung injury	72:88	Juglanin suppresses fibrosis and inflammation response caused by LPS in acute lung injury.
29532887	3	1	theme	natural	315:321	arg1	Juglanin					301:308	Juglanin	301:308	Juglanin	301:308	Juglanin is a natural product mainly isolated from green walnut husks of Juglans mandshurica, which isconsidered as the functional composition among a series of compounds.
29532887	3	1	theme	natural	315:321	arg1	product					323:329	a natural product	313:329	a natural product mainly isolated from green walnut husks of Juglans mandshurica, which isconsidered as the functional composition among a series of compounds	313:470	Juglanin is a natural product mainly isolated from green walnut husks of Juglans mandshurica, which isconsidered as the functional composition among a series of compounds.
29532887	5	2	theme	acute	776:780	arg1	injury					787:792	acute lung injury	776:792	acute lung injury	776:792	In our study, the protective effects and anti-inflammatory activity of juglanin were investigated in mice and lung cells treated by lipopolysaccharide (LPS) to reveal the possible mechanism by which juglanin attenuates acute lung injury.
29532887	11	3	dep	fibrosis	1502:1509	arg1	response					1528:1535	response	1528:1535	response	1528:1535	Our results indicate that juglanin has a protective role in LPS-triggered acute lung injury via suppression of fibrosis and inflammation response by NF-κB signaling pathways inactivation.
29532887	10	4	theme	cytokine	1272:1279	arg1	secretion					1281:1289	inflammatory cytokine secretion	1259:1289	inflammatory cytokine secretion	1259:1289	Additionally, juglanin markedly downregulated inflammatory cytokine secretion and phosphorylated nuclear factor-κB (NF-κB) expression via inhibiting IKKα/IκBα signaling pathway.
29532887	0	5	theme	acute	72:76	arg1	injury					83:88	acute lung injury	72:88	acute lung injury	72:88	Juglanin suppresses fibrosis and inflammation response caused by LPS in acute lung injury.
29532887	3	6	dep	Juglans	374:380	arg1	mandshurica					382:392	Juglans mandshurica, which isconsidered as the functional composition among a series of compounds	374:470	mandshurica	382:392	Juglanin is a natural product mainly isolated from green walnut husks of Juglans mandshurica, which isconsidered as the functional composition among a series of compounds.
29532887	3	6	dep	Juglans	374:380	arg1	isconsidered					401:412	isconsidered	401:412	isconsidered as the functional composition among a series of compounds	401:470	Juglanin is a natural product mainly isolated from green walnut husks of Juglans mandshurica, which isconsidered as the functional composition among a series of compounds.
29532887	5	7	theme	anti-inflammatory	598:614	arg1	activity					616:623	anti-inflammatory activity	598:623	anti-inflammatory activity	598:623	In our study, the protective effects and anti-inflammatory activity of juglanin were investigated in mice and lung cells treated by lipopolysaccharide (LPS) to reveal the possible mechanism by which juglanin attenuates acute lung injury.
29532887	1	8	theme	devastating	147:157	arg1	disorders					159:167	devastating disorders	147:167	devastating disorders linked to fibrosis progression and inflammation response	147:224	Acute lung injury in children is a complication showing devastating disorders linked to fibrosis progression and inflammation response.
29532887	7	9	theme	15 mg/kg	874:881	arg1	injection					887:895	15 mg/kg LPS injection	874:895	15 mg/kg LPS injection	874:895	The mouse model was established with 15 mg/kg LPS injection.
29532887	11	10	theme	fibrosis	1502:1509	arg1	suppression					1487:1497	suppression	1487:1497	suppression of fibrosis and inflammation response by NF-κB signaling pathways inactivation	1487:1576	Our results indicate that juglanin has a protective role in LPS-triggered acute lung injury via suppression of fibrosis and inflammation response by NF-κB signaling pathways inactivation.
29532887	3	11	theme	walnut	358:363	arg1	husks					365:369	green walnut husks	352:369	green walnut husks of Juglans mandshurica, which isconsidered as the functional composition among a series of compounds	352:470	Juglanin is a natural product mainly isolated from green walnut husks of Juglans mandshurica, which isconsidered as the functional composition among a series of compounds.
29532887	9	12	theme	transforming	1173:1184	arg1	TGF-β1					1204:1209	TGF-β1	1204:1209	TGF-β1	1204:1209	Compared to mice only treated with LPS, LPS-treated mice in the presence of juglanin developed less lung fibrosis with lower levels of α-smooth muscle-actin (α-SMA), collagen type I, collagen type III, and transforming growth factor-β1 (TGF-β1).
29532887	9	12	theme	transforming	1173:1184	arg1	factor-β1					1193:1201	transforming growth factor-β1	1173:1201	transforming growth factor-β1 (TGF-β1)	1173:1210	Compared to mice only treated with LPS, LPS-treated mice in the presence of juglanin developed less lung fibrosis with lower levels of α-smooth muscle-actin (α-SMA), collagen type I, collagen type III, and transforming growth factor-β1 (TGF-β1).
29532887	7	13	theme	LPS	883:885	arg1	injection					887:895	15 mg/kg LPS injection	874:895	15 mg/kg LPS injection	874:895	The mouse model was established with 15 mg/kg LPS injection.
29532887	3	14	attach	isolated	338:345	arg2	Juglanin					301:308	Juglanin	301:308	Juglanin	301:308	Juglanin is a natural product mainly isolated from green walnut husks of Juglans mandshurica, which isconsidered as the functional composition among a series of compounds.
29532887	3	14	attach	isolated	338:345	arg2	product					323:329	a natural product	313:329	a natural product mainly isolated from green walnut husks of Juglans mandshurica, which isconsidered as the functional composition among a series of compounds	313:470	Juglanin is a natural product mainly isolated from green walnut husks of Juglans mandshurica, which isconsidered as the functional composition among a series of compounds.
29532887	3	14	attach	isolated	338:345	arg1	husks					365:369	green walnut husks	352:369	green walnut husks of Juglans mandshurica, which isconsidered as the functional composition among a series of compounds	352:470	Juglanin is a natural product mainly isolated from green walnut husks of Juglans mandshurica, which isconsidered as the functional composition among a series of compounds.
29532887	9	15	theme	growth	1186:1191	arg1	TGF-β1					1204:1209	TGF-β1	1204:1209	TGF-β1	1204:1209	Compared to mice only treated with LPS, LPS-treated mice in the presence of juglanin developed less lung fibrosis with lower levels of α-smooth muscle-actin (α-SMA), collagen type I, collagen type III, and transforming growth factor-β1 (TGF-β1).
29532887	9	15	theme	growth	1186:1191	arg1	factor-β1					1193:1201	transforming growth factor-β1	1173:1201	transforming growth factor-β1 (TGF-β1)	1173:1210	Compared to mice only treated with LPS, LPS-treated mice in the presence of juglanin developed less lung fibrosis with lower levels of α-smooth muscle-actin (α-SMA), collagen type I, collagen type III, and transforming growth factor-β1 (TGF-β1).
29532887	2	16	theme	lung	288:291	arg1	injury					293:298	acute lung injury	282:298	acute lung injury	282:298	Fibrosis and inflammation response are two markers for acute lung injury.
29532887	10	17	theme	factor-κB	1318:1326	arg1	expression					1336:1345	phosphorylated nuclear factor-κB (NF-κB) expression	1295:1345	phosphorylated nuclear factor-κB (NF-κB) expression	1295:1345	Additionally, juglanin markedly downregulated inflammatory cytokine secretion and phosphorylated nuclear factor-κB (NF-κB) expression via inhibiting IKKα/IκBα signaling pathway.
29532887	5	18	theme	lung	782:785	arg1	injury					787:792	acute lung injury	776:792	acute lung injury	776:792	In our study, the protective effects and anti-inflammatory activity of juglanin were investigated in mice and lung cells treated by lipopolysaccharide (LPS) to reveal the possible mechanism by which juglanin attenuates acute lung injury.
29532887	11	19	theme	lung	1471:1474	arg1	injury					1476:1481	LPS-triggered acute lung injury	1451:1481	LPS-triggered acute lung injury	1451:1481	Our results indicate that juglanin has a protective role in LPS-triggered acute lung injury via suppression of fibrosis and inflammation response by NF-κB signaling pathways inactivation.
29532887	4	20	from	role	496:499	arg1	diseases					512:519	various diseases	504:519	various diseases	504:519	It exhibited effective role in various diseases by inhibiting inflammation response.
29532887	5	21	theme	juglanin	628:635	arg1	effects					586:592	the protective effects	571:592	the protective effects	571:592	In our study, the protective effects and anti-inflammatory activity of juglanin were investigated in mice and lung cells treated by lipopolysaccharide (LPS) to reveal the possible mechanism by which juglanin attenuates acute lung injury.
29532887	5	21	theme	juglanin	628:635	arg1	activity					616:623	anti-inflammatory activity	598:623	anti-inflammatory activity	598:623	In our study, the protective effects and anti-inflammatory activity of juglanin were investigated in mice and lung cells treated by lipopolysaccharide (LPS) to reveal the possible mechanism by which juglanin attenuates acute lung injury.
29532887	2	22	theme	acute	282:286	arg1	injury					293:298	acute lung injury	282:298	acute lung injury	282:298	Fibrosis and inflammation response are two markers for acute lung injury.
29532887	9	23	theme	lower	1086:1090	arg1	levels					1092:1097	lower levels	1086:1097	lower levels of α-smooth muscle-actin (α-SMA), collagen type I, collagen type III, and transforming growth factor-β1 (TGF-β1)	1086:1210	Compared to mice only treated with LPS, LPS-treated mice in the presence of juglanin developed less lung fibrosis with lower levels of α-smooth muscle-actin (α-SMA), collagen type I, collagen type III, and transforming growth factor-β1 (TGF-β1).
29532887	3	24	theme	green	352:356	arg1	husks					365:369	green walnut husks	352:369	green walnut husks of Juglans mandshurica, which isconsidered as the functional composition among a series of compounds	352:470	Juglanin is a natural product mainly isolated from green walnut husks of Juglans mandshurica, which isconsidered as the functional composition among a series of compounds.
29532887	10	25	theme	phosphorylated	1295:1308	arg1	expression					1336:1345	phosphorylated nuclear factor-κB (NF-κB) expression	1295:1345	phosphorylated nuclear factor-κB (NF-κB) expression	1295:1345	Additionally, juglanin markedly downregulated inflammatory cytokine secretion and phosphorylated nuclear factor-κB (NF-κB) expression via inhibiting IKKα/IκBα signaling pathway.
29532887	12	26	from	candidate	1613:1621	arg1	future					1683:1688	future	1683:1688	future	1683:1688	Thus, juglanin may be a potential candidate as dietary supplement for acute lung injury for children in future.
29532887	4	27	theme	effective	486:494	arg1	role					496:499	effective role	486:499	effective role in various diseases	486:519	It exhibited effective role in various diseases by inhibiting inflammation response.
29532887	5	28	theme	protective	575:584	arg1	effects					586:592	the protective effects	571:592	the protective effects	571:592	In our study, the protective effects and anti-inflammatory activity of juglanin were investigated in mice and lung cells treated by lipopolysaccharide (LPS) to reveal the possible mechanism by which juglanin attenuates acute lung injury.
29532887	11	29	theme	acute	1465:1469	arg1	injury					1476:1481	LPS-triggered acute lung injury	1451:1481	LPS-triggered acute lung injury	1451:1481	Our results indicate that juglanin has a protective role in LPS-triggered acute lung injury via suppression of fibrosis and inflammation response by NF-κB signaling pathways inactivation.
29532887	3	30	theme	functional	421:430	arg1	composition					432:442	the functional composition	417:442	the functional composition among a series of compounds	417:470	Juglanin is a natural product mainly isolated from green walnut husks of Juglans mandshurica, which isconsidered as the functional composition among a series of compounds.
29532887	12	31	theme	acute	1649:1653	arg1	injury					1660:1665	acute lung injury	1649:1665	acute lung injury	1649:1665	Thus, juglanin may be a potential candidate as dietary supplement for acute lung injury for children in future.
29532887	1	32	from	injury	102:107	arg1	children					112:119	children	112:119	children	112:119	Acute lung injury in children is a complication showing devastating disorders linked to fibrosis progression and inflammation response.
29532887	9	33	theme	α-smooth	1102:1109	arg1	muscle-actin					1111:1122	α-smooth muscle-actin	1102:1122	α-smooth muscle-actin (α-SMA)	1102:1130	Compared to mice only treated with LPS, LPS-treated mice in the presence of juglanin developed less lung fibrosis with lower levels of α-smooth muscle-actin (α-SMA), collagen type I, collagen type III, and transforming growth factor-β1 (TGF-β1).
29532887	9	33	theme	α-smooth	1102:1109	arg1	α-SMA					1125:1129	α-SMA	1125:1129	α-SMA	1125:1129	Compared to mice only treated with LPS, LPS-treated mice in the presence of juglanin developed less lung fibrosis with lower levels of α-smooth muscle-actin (α-SMA), collagen type I, collagen type III, and transforming growth factor-β1 (TGF-β1).
29532887	1	34	theme	fibrosis	179:186	arg1	progression					188:198	fibrosis progression	179:198	fibrosis progression	179:198	Acute lung injury in children is a complication showing devastating disorders linked to fibrosis progression and inflammation response.
29532887	12	35	theme	lung	1655:1658	arg1	injury					1660:1665	acute lung injury	1649:1665	acute lung injury	1649:1665	Thus, juglanin may be a potential candidate as dietary supplement for acute lung injury for children in future.
29532887	9	36	theme	factor-β1	1193:1201	arg1	levels					1092:1097	lower levels	1086:1097	lower levels of α-smooth muscle-actin (α-SMA), collagen type I, collagen type III, and transforming growth factor-β1 (TGF-β1)	1086:1210	Compared to mice only treated with LPS, LPS-treated mice in the presence of juglanin developed less lung fibrosis with lower levels of α-smooth muscle-actin (α-SMA), collagen type I, collagen type III, and transforming growth factor-β1 (TGF-β1).
29532887	1	37	theme	Acute	91:95	arg1	complication					126:137	a complication	124:137	a complication showing devastating disorders linked to fibrosis progression and inflammation response	124:224	Acute lung injury in children is a complication showing devastating disorders linked to fibrosis progression and inflammation response.
29532887	1	37	theme	Acute	91:95	arg1	injury					102:107	Acute lung injury	91:107	Acute lung injury in children	91:119	Acute lung injury in children is a complication showing devastating disorders linked to fibrosis progression and inflammation response.
29532887	7	38	theme	mouse	841:845	arg1	model					847:851	The mouse model	837:851	The mouse model	837:851	The mouse model was established with 15 mg/kg LPS injection.
29532887	3	39	theme	compounds	462:470	arg1	series					452:457	a series	450:457	a series of compounds	450:470	Juglanin is a natural product mainly isolated from green walnut husks of Juglans mandshurica, which isconsidered as the functional composition among a series of compounds.
29532887	3	40	theme	Juglans	374:380	arg1	husks					365:369	green walnut husks	352:369	green walnut husks of Juglans mandshurica, which isconsidered as the functional composition among a series of compounds	352:470	Juglanin is a natural product mainly isolated from green walnut husks of Juglans mandshurica, which isconsidered as the functional composition among a series of compounds.
29532887	9	41	theme	collagen	1133:1140	arg1	type I					1142:1147	collagen type I	1133:1147	collagen type I	1133:1147	Compared to mice only treated with LPS, LPS-treated mice in the presence of juglanin developed less lung fibrosis with lower levels of α-smooth muscle-actin (α-SMA), collagen type I, collagen type III, and transforming growth factor-β1 (TGF-β1).
29532887	1	42	attach	linked	169:174	arg2	disorders					159:167	devastating disorders	147:167	devastating disorders linked to fibrosis progression and inflammation response	147:224	Acute lung injury in children is a complication showing devastating disorders linked to fibrosis progression and inflammation response.
29532887	1	42	attach	linked	169:174	arg1	progression					188:198	fibrosis progression	179:198	fibrosis progression	179:198	Acute lung injury in children is a complication showing devastating disorders linked to fibrosis progression and inflammation response.
29532887	1	42	attach	linked	169:174	arg1	response					217:224	inflammation response	204:224	inflammation response	204:224	Acute lung injury in children is a complication showing devastating disorders linked to fibrosis progression and inflammation response.
29532887	1	43	theme	lung	97:100	arg1	complication					126:137	a complication	124:137	a complication showing devastating disorders linked to fibrosis progression and inflammation response	124:224	Acute lung injury in children is a complication showing devastating disorders linked to fibrosis progression and inflammation response.
29532887	1	43	theme	lung	97:100	arg1	injury					102:107	Acute lung injury	91:107	Acute lung injury in children	91:119	Acute lung injury in children is a complication showing devastating disorders linked to fibrosis progression and inflammation response.
29532887	5	44	theme	lung	667:670	arg1	cells					672:676	lung cells	667:676	lung cells	667:676	In our study, the protective effects and anti-inflammatory activity of juglanin were investigated in mice and lung cells treated by lipopolysaccharide (LPS) to reveal the possible mechanism by which juglanin attenuates acute lung injury.
29532887	10	45	theme	nuclear	1310:1316	arg1	expression					1336:1345	phosphorylated nuclear factor-κB (NF-κB) expression	1295:1345	phosphorylated nuclear factor-κB (NF-κB) expression	1295:1345	Additionally, juglanin markedly downregulated inflammatory cytokine secretion and phosphorylated nuclear factor-κB (NF-κB) expression via inhibiting IKKα/IκBα signaling pathway.
29532887	9	46	theme	lung	1067:1070	arg1	fibrosis					1072:1079	less lung fibrosis	1062:1079	less lung fibrosis	1062:1079	Compared to mice only treated with LPS, LPS-treated mice in the presence of juglanin developed less lung fibrosis with lower levels of α-smooth muscle-actin (α-SMA), collagen type I, collagen type III, and transforming growth factor-β1 (TGF-β1).
29532887	11	47	theme	inflammation	1515:1526	arg1	suppression					1487:1497	suppression	1487:1497	suppression of fibrosis and inflammation response by NF-κB signaling pathways inactivation	1487:1576	Our results indicate that juglanin has a protective role in LPS-triggered acute lung injury via suppression of fibrosis and inflammation response by NF-κB signaling pathways inactivation.
29532887	1	48	theme	inflammation	204:215	arg1	response					217:224	inflammation response	204:224	inflammation response	204:224	Acute lung injury in children is a complication showing devastating disorders linked to fibrosis progression and inflammation response.
29532887	0	49	theme	inflammation	33:44	arg1	response					46:53	fibrosis and inflammation response	20:53	fibrosis and inflammation response caused by LPS in acute lung injury	20:88	Juglanin suppresses fibrosis and inflammation response caused by LPS in acute lung injury.
29532887	4	50	theme	inflammation	535:545	arg1	response					547:554	inflammation response	535:554	inflammation response	535:554	It exhibited effective role in various diseases by inhibiting inflammation response.
29532887	9	51	theme	LPS-treated	1007:1017	arg1	mice					1019:1022	LPS-treated mice	1007:1022	LPS-treated mice in the presence of juglanin	1007:1050	Compared to mice only treated with LPS, LPS-treated mice in the presence of juglanin developed less lung fibrosis with lower levels of α-smooth muscle-actin (α-SMA), collagen type I, collagen type III, and transforming growth factor-β1 (TGF-β1).
29532887	11	52	from	role	1443:1446	arg1	injury					1476:1481	LPS-triggered acute lung injury	1451:1481	LPS-triggered acute lung injury	1451:1481	Our results indicate that juglanin has a protective role in LPS-triggered acute lung injury via suppression of fibrosis and inflammation response by NF-κB signaling pathways inactivation.
29532887	11	53	theme	protective	1432:1441	arg1	role					1443:1446	a protective role	1430:1446	a protective role in LPS-triggered acute lung injury	1430:1481	Our results indicate that juglanin has a protective role in LPS-triggered acute lung injury via suppression of fibrosis and inflammation response by NF-κB signaling pathways inactivation.
29532887	10	54	theme	signaling	1372:1380	arg1	pathway					1382:1388	IKKα/IκBα signaling pathway	1362:1388	IKKα/IκBα signaling pathway	1362:1388	Additionally, juglanin markedly downregulated inflammatory cytokine secretion and phosphorylated nuclear factor-κB (NF-κB) expression via inhibiting IKKα/IκBα signaling pathway.
29532887	9	55	theme	collagen	1150:1157	arg1	type III					1159:1166	collagen type III	1150:1166	collagen type III	1150:1166	Compared to mice only treated with LPS, LPS-treated mice in the presence of juglanin developed less lung fibrosis with lower levels of α-smooth muscle-actin (α-SMA), collagen type I, collagen type III, and transforming growth factor-β1 (TGF-β1).
29532887	5	56	theme	possible	728:735	arg1	mechanism					737:745	the possible mechanism	724:745	the possible mechanism by which juglanin attenuates acute lung injury	724:792	In our study, the protective effects and anti-inflammatory activity of juglanin were investigated in mice and lung cells treated by lipopolysaccharide (LPS) to reveal the possible mechanism by which juglanin attenuates acute lung injury.
29532887	10	57	theme	IKKα/IκBα	1362:1370	arg1	pathway					1382:1388	IKKα/IκBα signaling pathway	1362:1388	IKKα/IκBα signaling pathway	1362:1388	Additionally, juglanin markedly downregulated inflammatory cytokine secretion and phosphorylated nuclear factor-κB (NF-κB) expression via inhibiting IKKα/IκBα signaling pathway.
29532887	10	58	theme	NF-κB	1329:1333	arg1	expression					1336:1345	phosphorylated nuclear factor-κB (NF-κB) expression	1295:1345	phosphorylated nuclear factor-κB (NF-κB) expression	1295:1345	Additionally, juglanin markedly downregulated inflammatory cytokine secretion and phosphorylated nuclear factor-κB (NF-κB) expression via inhibiting IKKα/IκBα signaling pathway.
29532887	11	59	theme	NF-κB	1540:1544	arg1	pathways					1556:1563	NF-κB signaling pathways	1540:1563	NF-κB signaling pathways inactivation	1540:1576	Our results indicate that juglanin has a protective role in LPS-triggered acute lung injury via suppression of fibrosis and inflammation response by NF-κB signaling pathways inactivation.
29532887	9	60	from	mice	1019:1022	arg1	presence					1031:1038	the presence	1027:1038	the presence of juglanin	1027:1050	Compared to mice only treated with LPS, LPS-treated mice in the presence of juglanin developed less lung fibrosis with lower levels of α-smooth muscle-actin (α-SMA), collagen type I, collagen type III, and transforming growth factor-β1 (TGF-β1).
29532887	8	61	theme	infiltration	953:964	arg1	inflammation					932:943	the inflammation	928:943	the inflammation of cell infiltration	928:964	Juglanin dramatically reduced the inflammation of cell infiltration.
29532887	12	62	theme	dietary	1626:1632	arg1	supplement					1634:1643	dietary supplement	1626:1643	dietary supplement	1626:1643	Thus, juglanin may be a potential candidate as dietary supplement for acute lung injury for children in future.
29532887	8	63	theme	cell	948:951	arg1	infiltration					953:964	cell infiltration	948:964	cell infiltration	948:964	Juglanin dramatically reduced the inflammation of cell infiltration.
29532887	11	64	contain	has	1426:1428	arg1	juglanin					1417:1424	juglanin	1417:1424	juglanin	1417:1424	Our results indicate that juglanin has a protective role in LPS-triggered acute lung injury via suppression of fibrosis and inflammation response by NF-κB signaling pathways inactivation.
29532887	11	64	contain	has	1426:1428	arg2	role					1443:1446	a protective role	1430:1446	a protective role in LPS-triggered acute lung injury	1430:1481	Our results indicate that juglanin has a protective role in LPS-triggered acute lung injury via suppression of fibrosis and inflammation response by NF-κB signaling pathways inactivation.
29532887	10	65	theme	inflammatory	1259:1270	arg1	secretion					1281:1289	inflammatory cytokine secretion	1259:1289	inflammatory cytokine secretion	1259:1289	Additionally, juglanin markedly downregulated inflammatory cytokine secretion and phosphorylated nuclear factor-κB (NF-κB) expression via inhibiting IKKα/IκBα signaling pathway.
29532887	9	66	theme	type I	1142:1147	arg1	levels					1092:1097	lower levels	1086:1097	lower levels of α-smooth muscle-actin (α-SMA), collagen type I, collagen type III, and transforming growth factor-β1 (TGF-β1)	1086:1210	Compared to mice only treated with LPS, LPS-treated mice in the presence of juglanin developed less lung fibrosis with lower levels of α-smooth muscle-actin (α-SMA), collagen type I, collagen type III, and transforming growth factor-β1 (TGF-β1).
29532887	9	67	theme	muscle-actin	1111:1122	arg1	levels					1092:1097	lower levels	1086:1097	lower levels of α-smooth muscle-actin (α-SMA), collagen type I, collagen type III, and transforming growth factor-β1 (TGF-β1)	1086:1210	Compared to mice only treated with LPS, LPS-treated mice in the presence of juglanin developed less lung fibrosis with lower levels of α-smooth muscle-actin (α-SMA), collagen type I, collagen type III, and transforming growth factor-β1 (TGF-β1).
29532887	11	68	theme	LPS-triggered	1451:1463	arg1	injury					1476:1481	LPS-triggered acute lung injury	1451:1481	LPS-triggered acute lung injury	1451:1481	Our results indicate that juglanin has a protective role in LPS-triggered acute lung injury via suppression of fibrosis and inflammation response by NF-κB signaling pathways inactivation.
29532887	11	69	theme	signaling	1546:1554	arg1	pathways					1556:1563	NF-κB signaling pathways	1540:1563	NF-κB signaling pathways inactivation	1540:1576	Our results indicate that juglanin has a protective role in LPS-triggered acute lung injury via suppression of fibrosis and inflammation response by NF-κB signaling pathways inactivation.
29532887	2	70	dep	Fibrosis	227:234	arg1	response					253:260	response	253:260	response	253:260	Fibrosis and inflammation response are two markers for acute lung injury.
29532887	9	71	theme	juglanin	1043:1050	arg1	presence					1031:1038	the presence	1027:1038	the presence of juglanin	1027:1050	Compared to mice only treated with LPS, LPS-treated mice in the presence of juglanin developed less lung fibrosis with lower levels of α-smooth muscle-actin (α-SMA), collagen type I, collagen type III, and transforming growth factor-β1 (TGF-β1).
29532887	12	72	theme	potential	1603:1611	arg1	candidate					1613:1621	a potential candidate	1601:1621	a potential candidate as dietary supplement for acute lung injury for children	1601:1678	Thus, juglanin may be a potential candidate as dietary supplement for acute lung injury for children in future.
29532887	12	72	theme	potential	1603:1611	arg1	juglanin					1585:1592	juglanin	1585:1592	juglanin	1585:1592	Thus, juglanin may be a potential candidate as dietary supplement for acute lung injury for children in future.
29532887	4	73	theme	various	504:510	arg1	diseases					512:519	various diseases	504:519	various diseases	504:519	It exhibited effective role in various diseases by inhibiting inflammation response.
29532887	0	74	theme	fibrosis	20:27	arg1	response					46:53	fibrosis and inflammation response	20:53	fibrosis and inflammation response caused by LPS in acute lung injury	20:88	Juglanin suppresses fibrosis and inflammation response caused by LPS in acute lung injury.
29532887	9	75	theme	type III	1159:1166	arg1	levels					1092:1097	lower levels	1086:1097	lower levels of α-smooth muscle-actin (α-SMA), collagen type I, collagen type III, and transforming growth factor-β1 (TGF-β1)	1086:1210	Compared to mice only treated with LPS, LPS-treated mice in the presence of juglanin developed less lung fibrosis with lower levels of α-smooth muscle-actin (α-SMA), collagen type I, collagen type III, and transforming growth factor-β1 (TGF-β1).
29532887	11	76	theme	pathways	1556:1563	arg1	inactivation					1565:1576	NF-κB signaling pathways inactivation	1540:1576	NF-κB signaling pathways inactivation	1540:1576	Our results indicate that juglanin has a protective role in LPS-triggered acute lung injury via suppression of fibrosis and inflammation response by NF-κB signaling pathways inactivation.
31210845	9	0	theme	phenolic	1264:1271	arg1	acids					1273:1277	different phenolic acids	1254:1277	different phenolic acids	1254:1277	The presence of different phenolic acids and flavonoids was revealed, with rosmarinic acid as the most abundant component.
31210845	10	1	contain	possesses	1366:1374	arg2	activity					1403:1410	an interesting antioxidant activity	1376:1410	an interesting antioxidant activity	1376:1410	SSEE possesses an interesting antioxidant activity, as assessed through both the Oxygen Radical Absorbance Capacity (ORAC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays.
31210845	10	1	contain	possesses	1366:1374	arg1	SSEE					1361:1364	SSEE	1361:1364	SSEE	1361:1364	SSEE possesses an interesting antioxidant activity, as assessed through both the Oxygen Radical Absorbance Capacity (ORAC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays.
31210845	1	2	theme	present	144:150	arg1	research					152:159	the present research	140:159	the present research	140:159	The aim of the present research was to study the effects of an ethanolic extract of Salvia sagittata Ruiz & Pav (SSEE), an endemic Ecuadorian plant traditionally used to treat inflammation and different intestinal affections, on primary cultures of porcine aortic endothelial cells (pAECs).
31210845	10	3	theme	antioxidant	1391:1401	arg1	activity					1403:1410	an interesting antioxidant activity	1376:1410	an interesting antioxidant activity	1376:1410	SSEE possesses an interesting antioxidant activity, as assessed through both the Oxygen Radical Absorbance Capacity (ORAC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays.
31210845	1	4	theme	Ruiz	230:233	arg1	plant					271:275	an endemic Ecuadorian plant	249:275	an endemic Ecuadorian plant traditionally used to treat inflammation and different intestinal affections, on primary cultures of porcine aortic endothelial cells (pAECs)	249:417	The aim of the present research was to study the effects of an ethanolic extract of Salvia sagittata Ruiz & Pav (SSEE), an endemic Ecuadorian plant traditionally used to treat inflammation and different intestinal affections, on primary cultures of porcine aortic endothelial cells (pAECs).
31210845	1	4	theme	Ruiz	230:233	arg1	SSEE					242:245	SSEE	242:245	SSEE	242:245	The aim of the present research was to study the effects of an ethanolic extract of Salvia sagittata Ruiz & Pav (SSEE), an endemic Ecuadorian plant traditionally used to treat inflammation and different intestinal affections, on primary cultures of porcine aortic endothelial cells (pAECs).
31210845	1	4	theme	Ruiz	230:233	arg1	Pav					237:239	Salvia sagittata Ruiz & Pav	213:239	Salvia sagittata Ruiz & Pav (SSEE)	213:246	The aim of the present research was to study the effects of an ethanolic extract of Salvia sagittata Ruiz & Pav (SSEE), an endemic Ecuadorian plant traditionally used to treat inflammation and different intestinal affections, on primary cultures of porcine aortic endothelial cells (pAECs).
31210845	0	5	theme	Primary	75:81	arg1	Cultures					83:90	Primary Cultures	75:90	Primary Cultures of Porcine Aortic Endothelial Cells	75:126	In Vitro Anti-Inflammatory Effect of Salvia sagittata Ethanolic Extract on Primary Cultures of Porcine Aortic Endothelial Cells.
31210845	9	6	theme	acids	1273:1277	arg1	presence					1242:1249	The presence	1238:1249	The presence of different phenolic acids and flavonoids	1238:1292	The presence of different phenolic acids and flavonoids was revealed, with rosmarinic acid as the most abundant component.
31210845	3	7	theme	cellular	596:603	arg1	viability					605:613	cellular viability	596:613	cellular viability	596:613	SSEE did not negatively affect cellular viability at any concentration tested.
31210845	1	8	theme	cells	405:409	arg1	cultures					366:373	primary cultures	358:373	primary cultures of porcine aortic endothelial cells (pAECs)	358:417	The aim of the present research was to study the effects of an ethanolic extract of Salvia sagittata Ruiz & Pav (SSEE), an endemic Ecuadorian plant traditionally used to treat inflammation and different intestinal affections, on primary cultures of porcine aortic endothelial cells (pAECs).
31210845	1	9	theme	research	152:159	arg1	aim					133:135	The aim	129:135	The aim of the present research	129:159	The aim of the present research was to study the effects of an ethanolic extract of Salvia sagittata Ruiz & Pav (SSEE), an endemic Ecuadorian plant traditionally used to treat inflammation and different intestinal affections, on primary cultures of porcine aortic endothelial cells (pAECs).
31210845	12	10	theme	possible	1674:1681	arg1	support					1694:1700	a possible scientific support	1672:1700	a possible scientific support for the traditional therapeutic use of this plant	1672:1750	This represents the initial step in finding a possible scientific support for the traditional therapeutic use of this plant.
31210845	1	11	theme	&	235:235	arg1	plant					271:275	an endemic Ecuadorian plant	249:275	an endemic Ecuadorian plant traditionally used to treat inflammation and different intestinal affections, on primary cultures of porcine aortic endothelial cells (pAECs)	249:417	The aim of the present research was to study the effects of an ethanolic extract of Salvia sagittata Ruiz & Pav (SSEE), an endemic Ecuadorian plant traditionally used to treat inflammation and different intestinal affections, on primary cultures of porcine aortic endothelial cells (pAECs).
31210845	1	11	theme	&	235:235	arg1	SSEE					242:245	SSEE	242:245	SSEE	242:245	The aim of the present research was to study the effects of an ethanolic extract of Salvia sagittata Ruiz & Pav (SSEE), an endemic Ecuadorian plant traditionally used to treat inflammation and different intestinal affections, on primary cultures of porcine aortic endothelial cells (pAECs).
31210845	1	11	theme	&	235:235	arg1	Pav					237:239	Salvia sagittata Ruiz & Pav	213:239	Salvia sagittata Ruiz & Pav (SSEE)	213:246	The aim of the present research was to study the effects of an ethanolic extract of Salvia sagittata Ruiz & Pav (SSEE), an endemic Ecuadorian plant traditionally used to treat inflammation and different intestinal affections, on primary cultures of porcine aortic endothelial cells (pAECs).
31210845	0	12	theme	Porcine	95:101	arg1	Cells					122:126	Porcine Aortic Endothelial Cells	95:126	Porcine Aortic Endothelial Cells	95:126	In Vitro Anti-Inflammatory Effect of Salvia sagittata Ethanolic Extract on Primary Cultures of Porcine Aortic Endothelial Cells.
31210845	6	13	theme	Different	838:846	arg1	markers					848:854	Different markers	838:854	Different markers	838:854	Different markers involved in the inflammatory process, such as cytokines and protective molecules, were evaluated by real-time quantitative PCR and Western blot.
31210845	6	13	theme	Different	838:846	arg1	cytokines					902:910	cytokines	902:910	cytokines	902:910	Different markers involved in the inflammatory process, such as cytokines and protective molecules, were evaluated by real-time quantitative PCR and Western blot.
31210845	6	13	theme	Different	838:846	arg1	molecules					927:935	protective molecules	916:935	protective molecules	916:935	Different markers involved in the inflammatory process, such as cytokines and protective molecules, were evaluated by real-time quantitative PCR and Western blot.
31210845	7	14	theme	physiological	1041:1053	arg1	conditions					1055:1064	pAEC physiological conditions	1036:1064	pAEC physiological conditions	1036:1064	SSEE showed the ability to restore pAEC physiological conditions reducing interleukin-6 and increasing Heme Oxygenase-1 protein levels.
31210845	1	15	theme	Pav	237:239	arg1	extract					202:208	an ethanolic extract	189:208	an ethanolic extract of Salvia sagittata Ruiz & Pav (SSEE), an endemic Ecuadorian plant traditionally used to treat inflammation and different intestinal affections, on primary cultures of porcine aortic endothelial cells (pAECs)	189:417	The aim of the present research was to study the effects of an ethanolic extract of Salvia sagittata Ruiz & Pav (SSEE), an endemic Ecuadorian plant traditionally used to treat inflammation and different intestinal affections, on primary cultures of porcine aortic endothelial cells (pAECs).
31210845	11	16	theme	in	1593:1594	arg1	effect					1620:1625	an in vitro anti-inflammatory effect	1590:1625	an in vitro anti-inflammatory effect	1590:1625	In conclusion, results suggest that SSEE is endowed with an in vitro anti-inflammatory effect.
31210845	9	17	theme	flavonoids	1283:1292	arg1	presence					1242:1249	The presence	1238:1249	The presence of different phenolic acids and flavonoids	1238:1292	The presence of different phenolic acids and flavonoids was revealed, with rosmarinic acid as the most abundant component.
31210845	4	18	theme	Cell	644:647	arg1	cycle					649:653	Cell cycle	644:653	Cell cycle	644:653	Cell cycle was analyzed and no significant change was observed.
31210845	6	19	theme	protective	916:925	arg1	molecules					927:935	protective molecules	916:935	protective molecules	916:935	Different markers involved in the inflammatory process, such as cytokines and protective molecules, were evaluated by real-time quantitative PCR and Western blot.
31210845	0	20	theme	Endothelial	110:120	arg1	Cells					122:126	Porcine Aortic Endothelial Cells	95:126	Porcine Aortic Endothelial Cells	95:126	In Vitro Anti-Inflammatory Effect of Salvia sagittata Ethanolic Extract on Primary Cultures of Porcine Aortic Endothelial Cells.
31210845	1	21	theme	different	322:330	arg1	affections					343:352	different intestinal affections	322:352	different intestinal affections	322:352	The aim of the present research was to study the effects of an ethanolic extract of Salvia sagittata Ruiz & Pav (SSEE), an endemic Ecuadorian plant traditionally used to treat inflammation and different intestinal affections, on primary cultures of porcine aortic endothelial cells (pAECs).
31210845	2	22	theme	SSEE	501:504	arg1	concentrations					469:482	different concentrations	459:482	different concentrations (1-200 μg/mL) of SSEE for 24 h	459:513	pAECs were cultured in the presence of different concentrations (1-200 μg/mL) of SSEE for 24 h, and cytotoxicity was evaluated by the MTT assay.
31210845	2	22	theme	SSEE	501:504	arg1	1-200 μg/mL					485:495	1-200 μg/mL	485:495	1-200 μg/mL	485:495	pAECs were cultured in the presence of different concentrations (1-200 μg/mL) of SSEE for 24 h, and cytotoxicity was evaluated by the MTT assay.
31210845	6	23	theme	quantitative	966:977	arg1	PCR					979:981	real-time quantitative PCR	956:981	real-time quantitative PCR	956:981	Different markers involved in the inflammatory process, such as cytokines and protective molecules, were evaluated by real-time quantitative PCR and Western blot.
31210845	0	24	theme	Aortic	103:108	arg1	Cells					122:126	Porcine Aortic Endothelial Cells	95:126	Porcine Aortic Endothelial Cells	95:126	In Vitro Anti-Inflammatory Effect of Salvia sagittata Ethanolic Extract on Primary Cultures of Porcine Aortic Endothelial Cells.
31210845	1	25	theme	intestinal	332:341	arg1	affections					343:352	different intestinal affections	322:352	different intestinal affections	322:352	The aim of the present research was to study the effects of an ethanolic extract of Salvia sagittata Ruiz & Pav (SSEE), an endemic Ecuadorian plant traditionally used to treat inflammation and different intestinal affections, on primary cultures of porcine aortic endothelial cells (pAECs).
31210845	7	26	theme	Heme	1104:1107	arg1	Oxygenase-1					1109:1119	Heme Oxygenase-1	1104:1119	Heme Oxygenase-1 protein levels	1104:1134	SSEE showed the ability to restore pAEC physiological conditions reducing interleukin-6 and increasing Heme Oxygenase-1 protein levels.
31210845	12	27	theme	therapeutic	1722:1732	arg1	use					1734:1736	the traditional therapeutic use	1706:1736	the traditional therapeutic use of this plant	1706:1750	This represents the initial step in finding a possible scientific support for the traditional therapeutic use of this plant.
31210845	1	28	from	affections	343:352	arg1	cultures					366:373	primary cultures	358:373	primary cultures of porcine aortic endothelial cells (pAECs)	358:417	The aim of the present research was to study the effects of an ethanolic extract of Salvia sagittata Ruiz & Pav (SSEE), an endemic Ecuadorian plant traditionally used to treat inflammation and different intestinal affections, on primary cultures of porcine aortic endothelial cells (pAECs).
31210845	7	29	dep	restore	1028:1034	arg1	reducing					1066:1073	reducing	1066:1073	reducing interleukin-6	1066:1087	SSEE showed the ability to restore pAEC physiological conditions reducing interleukin-6 and increasing Heme Oxygenase-1 protein levels.
31210845	7	29	dep	restore	1028:1034	arg1	increasing					1093:1102	increasing	1093:1102	increasing Heme Oxygenase-1 protein levels	1093:1134	SSEE showed the ability to restore pAEC physiological conditions reducing interleukin-6 and increasing Heme Oxygenase-1 protein levels.
31210845	2	30	theme	concentrations	469:482	arg1	presence					447:454	the presence	443:454	the presence of different concentrations (1-200 μg/mL) of SSEE for 24 h	443:513	pAECs were cultured in the presence of different concentrations (1-200 μg/mL) of SSEE for 24 h, and cytotoxicity was evaluated by the MTT assay.
31210845	5	31	theme	SSEE	747:750	arg1	effects					736:742	the anti-inflammatory effects	714:742	the anti-inflammatory effects of SSEE on pAECs	714:759	Then, the anti-inflammatory effects of SSEE on pAECs were analyzed using a lipopolysaccharide (LPS) as the inflammatory stimulus.
31210845	11	32	with	endowed	1577:1583	arg1	effect					1620:1625	an in vitro anti-inflammatory effect	1590:1625	an in vitro anti-inflammatory effect	1590:1625	In conclusion, results suggest that SSEE is endowed with an in vitro anti-inflammatory effect.
31210845	10	33	dep	both	1433:1436	arg1	ORAC					1478:1481	ORAC	1478:1481	ORAC	1478:1481	SSEE possesses an interesting antioxidant activity, as assessed through both the Oxygen Radical Absorbance Capacity (ORAC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays.
31210845	10	33	dep	both	1433:1436	arg1	Capacity					1468:1475	the Oxygen Radical Absorbance Capacity	1438:1475	the Oxygen Radical Absorbance Capacity (ORAC)	1438:1482	SSEE possesses an interesting antioxidant activity, as assessed through both the Oxygen Radical Absorbance Capacity (ORAC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays.
31210845	12	34	theme	traditional	1710:1720	arg1	use					1734:1736	the traditional therapeutic use	1706:1736	the traditional therapeutic use of this plant	1706:1750	This represents the initial step in finding a possible scientific support for the traditional therapeutic use of this plant.
31210845	4	35	theme	significant	675:685	arg1	change					687:692	no significant change	672:692	no significant change	672:692	Cell cycle was analyzed and no significant change was observed.
31210845	12	36	theme	initial	1648:1654	arg1	step					1656:1659	the initial step	1644:1659	the initial step in finding a possible scientific support for the traditional therapeutic use of this plant	1644:1750	This represents the initial step in finding a possible scientific support for the traditional therapeutic use of this plant.
31210845	0	37	theme	In	0:1	arg1	Effect					27:32	In Vitro Anti-Inflammatory Effect	0:32	In Vitro Anti-Inflammatory Effect of Salvia sagittata Ethanolic Extract on Primary Cultures of Porcine Aortic Endothelial Cells	0:126	In Vitro Anti-Inflammatory Effect of Salvia sagittata Ethanolic Extract on Primary Cultures of Porcine Aortic Endothelial Cells.
31210845	7	38	theme	Oxygenase-1	1109:1119	arg1	levels					1129:1134	Heme Oxygenase-1 protein levels	1104:1134	Heme Oxygenase-1 protein levels	1104:1134	SSEE showed the ability to restore pAEC physiological conditions reducing interleukin-6 and increasing Heme Oxygenase-1 protein levels.
31210845	2	39	theme	MTT	554:556	arg1	assay					558:562	the MTT assay	550:562	the MTT assay	550:562	pAECs were cultured in the presence of different concentrations (1-200 μg/mL) of SSEE for 24 h, and cytotoxicity was evaluated by the MTT assay.
31210845	9	40	theme	abundant	1341:1348	arg1	component					1350:1358	the most abundant component	1332:1358	the most abundant component	1332:1358	The presence of different phenolic acids and flavonoids was revealed, with rosmarinic acid as the most abundant component.
31210845	9	41	theme	different	1254:1262	arg1	acids					1273:1277	different phenolic acids	1254:1277	different phenolic acids	1254:1277	The presence of different phenolic acids and flavonoids was revealed, with rosmarinic acid as the most abundant component.
31210845	11	42	theme	anti-inflammatory	1602:1618	arg1	effect					1620:1625	an in vitro anti-inflammatory effect	1590:1625	an in vitro anti-inflammatory effect	1590:1625	In conclusion, results suggest that SSEE is endowed with an in vitro anti-inflammatory effect.
31210845	8	43	theme	spectrophotometric	1211:1228	arg1	assays					1230:1235	HPLC-DAD and spectrophotometric assays	1198:1235	HPLC-DAD and spectrophotometric assays	1198:1235	The phytochemical composition of SSEE was also evaluated via HPLC-DAD and spectrophotometric assays.
31210845	7	44	theme	protein	1121:1127	arg1	levels					1129:1134	Heme Oxygenase-1 protein levels	1104:1134	Heme Oxygenase-1 protein levels	1104:1134	SSEE showed the ability to restore pAEC physiological conditions reducing interleukin-6 and increasing Heme Oxygenase-1 protein levels.
31210845	9	45	theme	rosmarinic	1313:1322	arg1	acid					1324:1327	rosmarinic acid	1313:1327	rosmarinic acid as the most abundant component	1313:1358	The presence of different phenolic acids and flavonoids was revealed, with rosmarinic acid as the most abundant component.
31210845	10	46	theme	interesting	1379:1389	arg1	activity					1403:1410	an interesting antioxidant activity	1376:1410	an interesting antioxidant activity	1376:1410	SSEE possesses an interesting antioxidant activity, as assessed through both the Oxygen Radical Absorbance Capacity (ORAC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays.
31210845	12	47	theme	plant	1746:1750	arg1	use					1734:1736	the traditional therapeutic use	1706:1736	the traditional therapeutic use of this plant	1706:1750	This represents the initial step in finding a possible scientific support for the traditional therapeutic use of this plant.
31210845	0	48	theme	Anti-Inflammatory	9:25	arg1	Effect					27:32	In Vitro Anti-Inflammatory Effect	0:32	In Vitro Anti-Inflammatory Effect of Salvia sagittata Ethanolic Extract on Primary Cultures of Porcine Aortic Endothelial Cells	0:126	In Vitro Anti-Inflammatory Effect of Salvia sagittata Ethanolic Extract on Primary Cultures of Porcine Aortic Endothelial Cells.
31210845	12	49	theme	scientific	1683:1692	arg1	support					1694:1700	a possible scientific support	1672:1700	a possible scientific support for the traditional therapeutic use of this plant	1672:1750	This represents the initial step in finding a possible scientific support for the traditional therapeutic use of this plant.
31210845	0	50	theme	Cells	122:126	arg1	Cultures					83:90	Primary Cultures	75:90	Primary Cultures of Porcine Aortic Endothelial Cells	75:126	In Vitro Anti-Inflammatory Effect of Salvia sagittata Ethanolic Extract on Primary Cultures of Porcine Aortic Endothelial Cells.
31210845	11	51	dep	in	1593:1594	arg1	vitro					1596:1600	vitro	1596:1600	vitro	1596:1600	In conclusion, results suggest that SSEE is endowed with an in vitro anti-inflammatory effect.
31210845	6	52	theme	Western	987:993	arg1	blot					995:998	Western blot	987:998	Western blot	987:998	Different markers involved in the inflammatory process, such as cytokines and protective molecules, were evaluated by real-time quantitative PCR and Western blot.
31210845	0	53	theme	Salvia	37:42	arg1	Extract					64:70	Salvia sagittata Ethanolic Extract	37:70	Salvia sagittata Ethanolic Extract	37:70	In Vitro Anti-Inflammatory Effect of Salvia sagittata Ethanolic Extract on Primary Cultures of Porcine Aortic Endothelial Cells.
31210845	1	54	from	inflammation	305:316	arg1	cultures					366:373	primary cultures	358:373	primary cultures of porcine aortic endothelial cells (pAECs)	358:417	The aim of the present research was to study the effects of an ethanolic extract of Salvia sagittata Ruiz & Pav (SSEE), an endemic Ecuadorian plant traditionally used to treat inflammation and different intestinal affections, on primary cultures of porcine aortic endothelial cells (pAECs).
31210845	1	55	theme	endemic	252:258	arg1	Pav					237:239	Salvia sagittata Ruiz & Pav	213:239	Salvia sagittata Ruiz & Pav (SSEE)	213:246	The aim of the present research was to study the effects of an ethanolic extract of Salvia sagittata Ruiz & Pav (SSEE), an endemic Ecuadorian plant traditionally used to treat inflammation and different intestinal affections, on primary cultures of porcine aortic endothelial cells (pAECs).
31210845	1	55	theme	endemic	252:258	arg1	plant					271:275	an endemic Ecuadorian plant	249:275	an endemic Ecuadorian plant traditionally used to treat inflammation and different intestinal affections, on primary cultures of porcine aortic endothelial cells (pAECs)	249:417	The aim of the present research was to study the effects of an ethanolic extract of Salvia sagittata Ruiz & Pav (SSEE), an endemic Ecuadorian plant traditionally used to treat inflammation and different intestinal affections, on primary cultures of porcine aortic endothelial cells (pAECs).
31210845	5	56	theme	anti-inflammatory	718:734	arg1	effects					736:742	the anti-inflammatory effects	714:742	the anti-inflammatory effects of SSEE on pAECs	714:759	Then, the anti-inflammatory effects of SSEE on pAECs were analyzed using a lipopolysaccharide (LPS) as the inflammatory stimulus.
31210845	1	57	theme	primary	358:364	arg1	cultures					366:373	primary cultures	358:373	primary cultures of porcine aortic endothelial cells (pAECs)	358:417	The aim of the present research was to study the effects of an ethanolic extract of Salvia sagittata Ruiz & Pav (SSEE), an endemic Ecuadorian plant traditionally used to treat inflammation and different intestinal affections, on primary cultures of porcine aortic endothelial cells (pAECs).
31210845	8	58	theme	SSEE	1170:1173	arg1	composition					1155:1165	The phytochemical composition	1137:1165	The phytochemical composition of SSEE	1137:1173	The phytochemical composition of SSEE was also evaluated via HPLC-DAD and spectrophotometric assays.
31210845	1	59	theme	ethanolic	192:200	arg1	extract					202:208	an ethanolic extract	189:208	an ethanolic extract of Salvia sagittata Ruiz & Pav (SSEE), an endemic Ecuadorian plant traditionally used to treat inflammation and different intestinal affections, on primary cultures of porcine aortic endothelial cells (pAECs)	189:417	The aim of the present research was to study the effects of an ethanolic extract of Salvia sagittata Ruiz & Pav (SSEE), an endemic Ecuadorian plant traditionally used to treat inflammation and different intestinal affections, on primary cultures of porcine aortic endothelial cells (pAECs).
31210845	0	60	theme	Ethanolic	54:62	arg1	Extract					64:70	Salvia sagittata Ethanolic Extract	37:70	Salvia sagittata Ethanolic Extract	37:70	In Vitro Anti-Inflammatory Effect of Salvia sagittata Ethanolic Extract on Primary Cultures of Porcine Aortic Endothelial Cells.
31210845	1	61	theme	Ecuadorian	260:269	arg1	Pav					237:239	Salvia sagittata Ruiz & Pav	213:239	Salvia sagittata Ruiz & Pav (SSEE)	213:246	The aim of the present research was to study the effects of an ethanolic extract of Salvia sagittata Ruiz & Pav (SSEE), an endemic Ecuadorian plant traditionally used to treat inflammation and different intestinal affections, on primary cultures of porcine aortic endothelial cells (pAECs).
31210845	1	61	theme	Ecuadorian	260:269	arg1	plant					271:275	an endemic Ecuadorian plant	249:275	an endemic Ecuadorian plant traditionally used to treat inflammation and different intestinal affections, on primary cultures of porcine aortic endothelial cells (pAECs)	249:417	The aim of the present research was to study the effects of an ethanolic extract of Salvia sagittata Ruiz & Pav (SSEE), an endemic Ecuadorian plant traditionally used to treat inflammation and different intestinal affections, on primary cultures of porcine aortic endothelial cells (pAECs).
31210845	10	62	dep	assays	1525:1530	arg1	2,2-diphenyl-1-picrylhydrazyl					1488:1516	2,2-diphenyl-1-picrylhydrazyl	1488:1516	2,2-diphenyl-1-picrylhydrazyl	1488:1516	SSEE possesses an interesting antioxidant activity, as assessed through both the Oxygen Radical Absorbance Capacity (ORAC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays.
31210845	10	62	dep	assays	1525:1530	arg1	both					1433:1436	both	1433:1436	both	1433:1436	SSEE possesses an interesting antioxidant activity, as assessed through both the Oxygen Radical Absorbance Capacity (ORAC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays.
31210845	0	63	theme	sagittata	44:52	arg1	Extract					64:70	Salvia sagittata Ethanolic Extract	37:70	Salvia sagittata Ethanolic Extract	37:70	In Vitro Anti-Inflammatory Effect of Salvia sagittata Ethanolic Extract on Primary Cultures of Porcine Aortic Endothelial Cells.
31210845	6	64	theme	inflammatory	872:883	arg1	process					885:891	the inflammatory process	868:891	the inflammatory process	868:891	Different markers involved in the inflammatory process, such as cytokines and protective molecules, were evaluated by real-time quantitative PCR and Western blot.
31210845	5	65	from	effects	736:742	arg1	pAECs					755:759	pAECs	755:759	pAECs	755:759	Then, the anti-inflammatory effects of SSEE on pAECs were analyzed using a lipopolysaccharide (LPS) as the inflammatory stimulus.
31210845	7	66	theme	pAEC	1036:1039	arg1	conditions					1055:1064	pAEC physiological conditions	1036:1064	pAEC physiological conditions	1036:1064	SSEE showed the ability to restore pAEC physiological conditions reducing interleukin-6 and increasing Heme Oxygenase-1 protein levels.
31210845	10	67	theme	Radical	1449:1455	arg1	ORAC					1478:1481	ORAC	1478:1481	ORAC	1478:1481	SSEE possesses an interesting antioxidant activity, as assessed through both the Oxygen Radical Absorbance Capacity (ORAC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays.
31210845	10	67	theme	Radical	1449:1455	arg1	Capacity					1468:1475	the Oxygen Radical Absorbance Capacity	1438:1475	the Oxygen Radical Absorbance Capacity (ORAC)	1438:1482	SSEE possesses an interesting antioxidant activity, as assessed through both the Oxygen Radical Absorbance Capacity (ORAC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays.
31210845	10	68	dep	Radical	1449:1455	arg1	Oxygen					1442:1447	Oxygen	1442:1447	Oxygen	1442:1447	SSEE possesses an interesting antioxidant activity, as assessed through both the Oxygen Radical Absorbance Capacity (ORAC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays.
31210845	1	69	theme	porcine	378:384	arg1	pAECs					412:416	pAECs	412:416	pAECs	412:416	The aim of the present research was to study the effects of an ethanolic extract of Salvia sagittata Ruiz & Pav (SSEE), an endemic Ecuadorian plant traditionally used to treat inflammation and different intestinal affections, on primary cultures of porcine aortic endothelial cells (pAECs).
31210845	1	69	theme	porcine	378:384	arg1	cells					405:409	porcine aortic endothelial cells	378:409	porcine aortic endothelial cells (pAECs)	378:417	The aim of the present research was to study the effects of an ethanolic extract of Salvia sagittata Ruiz & Pav (SSEE), an endemic Ecuadorian plant traditionally used to treat inflammation and different intestinal affections, on primary cultures of porcine aortic endothelial cells (pAECs).
31210845	2	70	theme	different	459:467	arg1	concentrations					469:482	different concentrations	459:482	different concentrations (1-200 μg/mL) of SSEE for 24 h	459:513	pAECs were cultured in the presence of different concentrations (1-200 μg/mL) of SSEE for 24 h, and cytotoxicity was evaluated by the MTT assay.
31210845	2	70	theme	different	459:467	arg1	1-200 μg/mL					485:495	1-200 μg/mL	485:495	1-200 μg/mL	485:495	pAECs were cultured in the presence of different concentrations (1-200 μg/mL) of SSEE for 24 h, and cytotoxicity was evaluated by the MTT assay.
31210845	1	71	theme	extract	202:208	arg1	effects					178:184	the effects	174:184	the effects of an ethanolic extract of Salvia sagittata Ruiz & Pav (SSEE), an endemic Ecuadorian plant traditionally used to treat inflammation and different intestinal affections, on primary cultures of porcine aortic endothelial cells (pAECs)	174:417	The aim of the present research was to study the effects of an ethanolic extract of Salvia sagittata Ruiz & Pav (SSEE), an endemic Ecuadorian plant traditionally used to treat inflammation and different intestinal affections, on primary cultures of porcine aortic endothelial cells (pAECs).
31210845	1	72	theme	aortic	386:391	arg1	pAECs					412:416	pAECs	412:416	pAECs	412:416	The aim of the present research was to study the effects of an ethanolic extract of Salvia sagittata Ruiz & Pav (SSEE), an endemic Ecuadorian plant traditionally used to treat inflammation and different intestinal affections, on primary cultures of porcine aortic endothelial cells (pAECs).
31210845	1	72	theme	aortic	386:391	arg1	cells					405:409	porcine aortic endothelial cells	378:409	porcine aortic endothelial cells (pAECs)	378:417	The aim of the present research was to study the effects of an ethanolic extract of Salvia sagittata Ruiz & Pav (SSEE), an endemic Ecuadorian plant traditionally used to treat inflammation and different intestinal affections, on primary cultures of porcine aortic endothelial cells (pAECs).
31210845	8	73	theme	phytochemical	1141:1153	arg1	composition					1155:1165	The phytochemical composition	1137:1165	The phytochemical composition of SSEE	1137:1173	The phytochemical composition of SSEE was also evaluated via HPLC-DAD and spectrophotometric assays.
31210845	10	74	theme	Absorbance	1457:1466	arg1	ORAC					1478:1481	ORAC	1478:1481	ORAC	1478:1481	SSEE possesses an interesting antioxidant activity, as assessed through both the Oxygen Radical Absorbance Capacity (ORAC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays.
31210845	10	74	theme	Absorbance	1457:1466	arg1	Capacity					1468:1475	the Oxygen Radical Absorbance Capacity	1438:1475	the Oxygen Radical Absorbance Capacity (ORAC)	1438:1482	SSEE possesses an interesting antioxidant activity, as assessed through both the Oxygen Radical Absorbance Capacity (ORAC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays.
31210845	6	75	theme	real-time	956:964	arg1	PCR					979:981	real-time quantitative PCR	956:981	real-time quantitative PCR	956:981	Different markers involved in the inflammatory process, such as cytokines and protective molecules, were evaluated by real-time quantitative PCR and Western blot.
31210845	10	76	dep	2,2-diphenyl-1-picrylhydrazyl	1488:1516	arg1	DPPH					1519:1522	DPPH	1519:1522	DPPH	1519:1522	SSEE possesses an interesting antioxidant activity, as assessed through both the Oxygen Radical Absorbance Capacity (ORAC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays.
31210845	1	77	theme	endothelial	393:403	arg1	pAECs					412:416	pAECs	412:416	pAECs	412:416	The aim of the present research was to study the effects of an ethanolic extract of Salvia sagittata Ruiz & Pav (SSEE), an endemic Ecuadorian plant traditionally used to treat inflammation and different intestinal affections, on primary cultures of porcine aortic endothelial cells (pAECs).
31210845	1	77	theme	endothelial	393:403	arg1	cells					405:409	porcine aortic endothelial cells	378:409	porcine aortic endothelial cells (pAECs)	378:417	The aim of the present research was to study the effects of an ethanolic extract of Salvia sagittata Ruiz & Pav (SSEE), an endemic Ecuadorian plant traditionally used to treat inflammation and different intestinal affections, on primary cultures of porcine aortic endothelial cells (pAECs).
31210845	8	78	theme	HPLC-DAD	1198:1205	arg1	assays					1230:1235	HPLC-DAD and spectrophotometric assays	1198:1235	HPLC-DAD and spectrophotometric assays	1198:1235	The phytochemical composition of SSEE was also evaluated via HPLC-DAD and spectrophotometric assays.
31210845	1	79	theme	Salvia	213:218	arg1	plant					271:275	an endemic Ecuadorian plant	249:275	an endemic Ecuadorian plant traditionally used to treat inflammation and different intestinal affections, on primary cultures of porcine aortic endothelial cells (pAECs)	249:417	The aim of the present research was to study the effects of an ethanolic extract of Salvia sagittata Ruiz & Pav (SSEE), an endemic Ecuadorian plant traditionally used to treat inflammation and different intestinal affections, on primary cultures of porcine aortic endothelial cells (pAECs).
31210845	1	79	theme	Salvia	213:218	arg1	SSEE					242:245	SSEE	242:245	SSEE	242:245	The aim of the present research was to study the effects of an ethanolic extract of Salvia sagittata Ruiz & Pav (SSEE), an endemic Ecuadorian plant traditionally used to treat inflammation and different intestinal affections, on primary cultures of porcine aortic endothelial cells (pAECs).
31210845	1	79	theme	Salvia	213:218	arg1	Pav					237:239	Salvia sagittata Ruiz & Pav	213:239	Salvia sagittata Ruiz & Pav (SSEE)	213:246	The aim of the present research was to study the effects of an ethanolic extract of Salvia sagittata Ruiz & Pav (SSEE), an endemic Ecuadorian plant traditionally used to treat inflammation and different intestinal affections, on primary cultures of porcine aortic endothelial cells (pAECs).
31210845	0	80	theme	Extract	64:70	arg1	Effect					27:32	In Vitro Anti-Inflammatory Effect	0:32	In Vitro Anti-Inflammatory Effect of Salvia sagittata Ethanolic Extract on Primary Cultures of Porcine Aortic Endothelial Cells	0:126	In Vitro Anti-Inflammatory Effect of Salvia sagittata Ethanolic Extract on Primary Cultures of Porcine Aortic Endothelial Cells.
31210845	0	81	from	Effect	27:32	arg1	Cultures					83:90	Primary Cultures	75:90	Primary Cultures of Porcine Aortic Endothelial Cells	75:126	In Vitro Anti-Inflammatory Effect of Salvia sagittata Ethanolic Extract on Primary Cultures of Porcine Aortic Endothelial Cells.
31210845	0	82	dep	In	0:1	arg1	Vitro					3:7	Vitro	3:7	Vitro	3:7	In Vitro Anti-Inflammatory Effect of Salvia sagittata Ethanolic Extract on Primary Cultures of Porcine Aortic Endothelial Cells.
31210845	5	83	theme	inflammatory	815:826	arg1	stimulus					828:835	the inflammatory stimulus	811:835	the inflammatory stimulus	811:835	Then, the anti-inflammatory effects of SSEE on pAECs were analyzed using a lipopolysaccharide (LPS) as the inflammatory stimulus.
31210845	5	83	theme	inflammatory	815:826	arg1	lipopolysaccharide					783:800	a lipopolysaccharide	781:800	a lipopolysaccharide (LPS)	781:806	Then, the anti-inflammatory effects of SSEE on pAECs were analyzed using a lipopolysaccharide (LPS) as the inflammatory stimulus.
31210845	1	84	theme	sagittata	220:228	arg1	plant					271:275	an endemic Ecuadorian plant	249:275	an endemic Ecuadorian plant traditionally used to treat inflammation and different intestinal affections, on primary cultures of porcine aortic endothelial cells (pAECs)	249:417	The aim of the present research was to study the effects of an ethanolic extract of Salvia sagittata Ruiz & Pav (SSEE), an endemic Ecuadorian plant traditionally used to treat inflammation and different intestinal affections, on primary cultures of porcine aortic endothelial cells (pAECs).
31210845	1	84	theme	sagittata	220:228	arg1	SSEE					242:245	SSEE	242:245	SSEE	242:245	The aim of the present research was to study the effects of an ethanolic extract of Salvia sagittata Ruiz & Pav (SSEE), an endemic Ecuadorian plant traditionally used to treat inflammation and different intestinal affections, on primary cultures of porcine aortic endothelial cells (pAECs).
31210845	1	84	theme	sagittata	220:228	arg1	Pav					237:239	Salvia sagittata Ruiz & Pav	213:239	Salvia sagittata Ruiz & Pav (SSEE)	213:246	The aim of the present research was to study the effects of an ethanolic extract of Salvia sagittata Ruiz & Pav (SSEE), an endemic Ecuadorian plant traditionally used to treat inflammation and different intestinal affections, on primary cultures of porcine aortic endothelial cells (pAECs).
29670036	0	0	theme	Phyllanthus	91:101	arg1	brasiliensis					103:114	Phyllanthus brasiliensis	91:114	Phyllanthus brasiliensis	91:114	Anti-Inflammatory and Antinociceptive Studies of Hydroalcoholic Extract from the Leaves of Phyllanthus brasiliensis (Aubl.)
29670036	5	1	theme	2D	565:566	arg1	experiments					572:582	1D and 2D NMR experiments	558:582	1D and 2D NMR experiments	558:582	1D and 2D NMR experiments and HRMS were used to elucidate the structure of the new compound.
29670036	4	2	theme	known	518:522	arg1	lignans					524:530	six known lignans	514:530	six known lignans	514:530	A new arylnaphthalene lignan glycoside, 5-O-β-d-glucopyranosyljusticidin B, together with six known lignans, were isolated from HEPB.
29670036	10	3	theme	neurogenic	1076:1085	arg1	phases					1104:1109	the neurogenic and inflammatory phases	1072:1109	the neurogenic and inflammatory phases of formalin-induced linking	1072:1137	HEPB and its fractions from FR6 to FR10 (FR6-10) inhibited the neurogenic and inflammatory phases of formalin-induced linking, demonstrating its antinociceptive activity.
29670036	6	4	from	toxicity	728:735	arg1	mice					760:763	mice	760:763	mice	760:763	HEPB toxicity and antinociceptive activity were evaluated through acute oral toxicity and formalin models in mice, respectively.
29670036	2	5	theme	B	180:180	arg1	Lignans					196:202	Six Other Lignans	186:202	Six Other Lignans	186:202	and Isolation of 5-O-β-d-Glucopyranosyljusticidin B and Six Other Lignans.
29670036	2	5	theme	B	180:180	arg1	Isolation					134:142	Isolation	134:142	Isolation of 5-O-β-d-Glucopyranosyljusticidin B	134:180	and Isolation of 5-O-β-d-Glucopyranosyljusticidin B and Six Other Lignans.
29670036	3	6	theme	study	221:225	arg1	aim					209:211	The aim	205:211	The aim of this study	205:225	The aim of this study was to investigate the chemical composition and the antiinflammatory/antinociceptive properties of the hydroalcoholic extract derived from the leaves of Phyllanthus brasiliensis (HEPB) in rodents.
29670036	4	7	theme	arylnaphthalene	430:444	arg1	glycoside					453:461	A new arylnaphthalene lignan glycoside	424:461	A new arylnaphthalene lignan glycoside	424:461	A new arylnaphthalene lignan glycoside, 5-O-β-d-glucopyranosyljusticidin B, together with six known lignans, were isolated from HEPB.
29670036	4	7	theme	arylnaphthalene	430:444	arg1	B					497:497	5-O-β-d-glucopyranosyljusticidin B	464:497	5-O-β-d-glucopyranosyljusticidin B	464:497	A new arylnaphthalene lignan glycoside, 5-O-β-d-glucopyranosyljusticidin B, together with six known lignans, were isolated from HEPB.
29670036	6	8	theme	formalin	741:748	arg1	models					750:755	formalin models	741:755	formalin models	741:755	HEPB toxicity and antinociceptive activity were evaluated through acute oral toxicity and formalin models in mice, respectively.
29670036	2	9	theme	5-O-β-d-Glucopyranosyljusticidin	147:178	arg1	B					180:180	5-O-β-d-Glucopyranosyljusticidin B	147:180	5-O-β-d-Glucopyranosyljusticidin B	147:180	and Isolation of 5-O-β-d-Glucopyranosyljusticidin B and Six Other Lignans.
29670036	3	10	theme	chemical	250:257	arg1	composition					259:269	the chemical composition	246:269	the chemical composition	246:269	The aim of this study was to investigate the chemical composition and the antiinflammatory/antinociceptive properties of the hydroalcoholic extract derived from the leaves of Phyllanthus brasiliensis (HEPB) in rodents.
29670036	4	11	theme	new	426:428	arg1	glycoside					453:461	A new arylnaphthalene lignan glycoside	424:461	A new arylnaphthalene lignan glycoside	424:461	A new arylnaphthalene lignan glycoside, 5-O-β-d-glucopyranosyljusticidin B, together with six known lignans, were isolated from HEPB.
29670036	4	11	theme	new	426:428	arg1	B					497:497	5-O-β-d-glucopyranosyljusticidin B	464:497	5-O-β-d-glucopyranosyljusticidin B	464:497	A new arylnaphthalene lignan glycoside, 5-O-β-d-glucopyranosyljusticidin B, together with six known lignans, were isolated from HEPB.
29670036	9	12	theme	Oral	923:926	arg1	administration					928:941	Oral administration	923:941	Oral administration of HEPB	923:949	Oral administration of HEPB reduced paw edema induced by carrageenan, but not by dextran.
29670036	5	13	theme	compound	641:648	arg1	structure					620:628	the structure	616:628	the structure of the new compound	616:648	1D and 2D NMR experiments and HRMS were used to elucidate the structure of the new compound.
29670036	5	14	used	used	598:601	arg2	HRMS					588:591	HRMS	588:591	HRMS	588:591	1D and 2D NMR experiments and HRMS were used to elucidate the structure of the new compound.
29670036	5	14	used	used	598:601	arg2	experiments					572:582	1D and 2D NMR experiments	558:582	1D and 2D NMR experiments	558:582	1D and 2D NMR experiments and HRMS were used to elucidate the structure of the new compound.
29670036	3	15	attach	derived	353:359	arg2	extract					345:351	the hydroalcoholic extract	326:351	the hydroalcoholic extract derived from the leaves of Phyllanthus brasiliensis (HEPB) in rodents	326:421	The aim of this study was to investigate the chemical composition and the antiinflammatory/antinociceptive properties of the hydroalcoholic extract derived from the leaves of Phyllanthus brasiliensis (HEPB) in rodents.
29670036	3	15	attach	derived	353:359	arg1	the					366:368	the	366:368	the	366:368	The aim of this study was to investigate the chemical composition and the antiinflammatory/antinociceptive properties of the hydroalcoholic extract derived from the leaves of Phyllanthus brasiliensis (HEPB) in rodents.
29670036	10	16	theme	antinociceptive	1158:1172	arg1	activity					1174:1181	its antinociceptive activity	1154:1181	its antinociceptive activity	1154:1181	HEPB and its fractions from FR6 to FR10 (FR6-10) inhibited the neurogenic and inflammatory phases of formalin-induced linking, demonstrating its antinociceptive activity.
29670036	6	17	theme	oral	723:726	arg1	toxicity					728:735	acute oral toxicity	717:735	acute oral toxicity	717:735	HEPB toxicity and antinociceptive activity were evaluated through acute oral toxicity and formalin models in mice, respectively.
29670036	0	18	theme	Anti-Inflammatory	0:16	arg1	Studies					38:44	Anti-Inflammatory and Antinociceptive Studies	0:44	Anti-Inflammatory and Antinociceptive Studies of Hydroalcoholic Extract from the Leaves of Phyllanthus brasiliensis	0:114	Anti-Inflammatory and Antinociceptive Studies of Hydroalcoholic Extract from the Leaves of Phyllanthus brasiliensis (Aubl.)
29670036	7	19	theme	carrageenan-	838:849	arg1	models					881:886	carrageenan- and dextran-induced paw edema models	838:886	carrageenan- and dextran-induced paw edema models in rats	838:894	The anti-inflammatory effects of HEPB were assessed using carrageenan- and dextran-induced paw edema models in rats.
29670036	5	20	theme	new	637:639	arg1	compound					641:648	the new compound	633:648	the new compound	633:648	1D and 2D NMR experiments and HRMS were used to elucidate the structure of the new compound.
29670036	6	21	theme	acute	717:721	arg1	toxicity					728:735	acute oral toxicity	717:735	acute oral toxicity	717:735	HEPB toxicity and antinociceptive activity were evaluated through acute oral toxicity and formalin models in mice, respectively.
29670036	3	22	theme	antiinflammatory/antinociceptive	279:310	arg1	properties					312:321	the antiinflammatory/antinociceptive properties	275:321	the antiinflammatory/antinociceptive properties of the hydroalcoholic extract derived from the leaves of Phyllanthus brasiliensis (HEPB) in rodents	275:421	The aim of this study was to investigate the chemical composition and the antiinflammatory/antinociceptive properties of the hydroalcoholic extract derived from the leaves of Phyllanthus brasiliensis (HEPB) in rodents.
29670036	11	23	theme	Phyllanthus	1226:1236	arg1	brasiliensis					1238:1249	Phyllanthus brasiliensis	1226:1249	Phyllanthus brasiliensis	1226:1249	These results indicated that lignans from Phyllanthus brasiliensis exerted antinociceptive/anti-inflammatory effects not related to the histaminergic pathway.
29670036	0	24	theme	Antinociceptive	22:36	arg1	Studies					38:44	Anti-Inflammatory and Antinociceptive Studies	0:44	Anti-Inflammatory and Antinociceptive Studies of Hydroalcoholic Extract from the Leaves of Phyllanthus brasiliensis	0:114	Anti-Inflammatory and Antinociceptive Studies of Hydroalcoholic Extract from the Leaves of Phyllanthus brasiliensis (Aubl.)
29670036	0	25	dep	the	77:79	arg1	Leaves					81:86	Leaves	81:86	Leaves	81:86	Anti-Inflammatory and Antinociceptive Studies of Hydroalcoholic Extract from the Leaves of Phyllanthus brasiliensis (Aubl.)
29670036	7	26	theme	dextran-induced	855:869	arg1	edema					875:879	dextran-induced paw edema	855:879	dextran-induced paw edema	855:879	The anti-inflammatory effects of HEPB were assessed using carrageenan- and dextran-induced paw edema models in rats.
29670036	7	27	theme	edema	875:879	arg1	models					881:886	carrageenan- and dextran-induced paw edema models	838:886	carrageenan- and dextran-induced paw edema models in rats	838:894	The anti-inflammatory effects of HEPB were assessed using carrageenan- and dextran-induced paw edema models in rats.
29670036	0	28	theme	Hydroalcoholic	49:62	arg1	Extract					64:70	Hydroalcoholic Extract	49:70	Hydroalcoholic Extract from the Leaves of Phyllanthus brasiliensis	49:114	Anti-Inflammatory and Antinociceptive Studies of Hydroalcoholic Extract from the Leaves of Phyllanthus brasiliensis (Aubl.)
29670036	11	29	theme	related	1305:1311	arg1	effects					1293:1299	antinociceptive/anti-inflammatory effects	1259:1299	antinociceptive/anti-inflammatory effects not related to the histaminergic pathway	1259:1340	These results indicated that lignans from Phyllanthus brasiliensis exerted antinociceptive/anti-inflammatory effects not related to the histaminergic pathway.
29670036	3	30	dep	the	366:368	arg1	leaves					370:375	leaves	370:375	leaves	370:375	The aim of this study was to investigate the chemical composition and the antiinflammatory/antinociceptive properties of the hydroalcoholic extract derived from the leaves of Phyllanthus brasiliensis (HEPB) in rodents.
29670036	2	31	theme	Other	190:194	arg1	Lignans					196:202	Six Other Lignans	186:202	Six Other Lignans	186:202	and Isolation of 5-O-β-d-Glucopyranosyljusticidin B and Six Other Lignans.
29670036	7	32	theme	paw	871:873	arg1	edema					875:879	dextran-induced paw edema	855:879	dextran-induced paw edema	855:879	The anti-inflammatory effects of HEPB were assessed using carrageenan- and dextran-induced paw edema models in rats.
29670036	7	33	theme	HEPB	813:816	arg1	effects					802:808	The anti-inflammatory effects	780:808	The anti-inflammatory effects of HEPB	780:816	The anti-inflammatory effects of HEPB were assessed using carrageenan- and dextran-induced paw edema models in rats.
29670036	5	34	theme	NMR	568:570	arg1	experiments					572:582	1D and 2D NMR experiments	558:582	1D and 2D NMR experiments	558:582	1D and 2D NMR experiments and HRMS were used to elucidate the structure of the new compound.
29670036	3	35	theme	hydroalcoholic	330:343	arg1	extract					345:351	the hydroalcoholic extract	326:351	the hydroalcoholic extract derived from the leaves of Phyllanthus brasiliensis (HEPB) in rodents	326:421	The aim of this study was to investigate the chemical composition and the antiinflammatory/antinociceptive properties of the hydroalcoholic extract derived from the leaves of Phyllanthus brasiliensis (HEPB) in rodents.
29670036	0	36	from	the	77:79	arg1	Studies					38:44	Anti-Inflammatory and Antinociceptive Studies	0:44	Anti-Inflammatory and Antinociceptive Studies of Hydroalcoholic Extract from the Leaves of Phyllanthus brasiliensis	0:114	Anti-Inflammatory and Antinociceptive Studies of Hydroalcoholic Extract from the Leaves of Phyllanthus brasiliensis (Aubl.)
29670036	0	36	from	the	77:79	arg1	Extract					64:70	Hydroalcoholic Extract	49:70	Hydroalcoholic Extract from the Leaves of Phyllanthus brasiliensis	49:114	Anti-Inflammatory and Antinociceptive Studies of Hydroalcoholic Extract from the Leaves of Phyllanthus brasiliensis (Aubl.)
29670036	10	37	theme	inflammatory	1091:1102	arg1	phases					1104:1109	the neurogenic and inflammatory phases	1072:1109	the neurogenic and inflammatory phases of formalin-induced linking	1072:1137	HEPB and its fractions from FR6 to FR10 (FR6-10) inhibited the neurogenic and inflammatory phases of formalin-induced linking, demonstrating its antinociceptive activity.
29670036	0	38	theme	Extract	64:70	arg1	Studies					38:44	Anti-Inflammatory and Antinociceptive Studies	0:44	Anti-Inflammatory and Antinociceptive Studies of Hydroalcoholic Extract from the Leaves of Phyllanthus brasiliensis	0:114	Anti-Inflammatory and Antinociceptive Studies of Hydroalcoholic Extract from the Leaves of Phyllanthus brasiliensis (Aubl.)
29670036	3	39	theme	extract	345:351	arg1	properties					312:321	the antiinflammatory/antinociceptive properties	275:321	the antiinflammatory/antinociceptive properties of the hydroalcoholic extract derived from the leaves of Phyllanthus brasiliensis (HEPB) in rodents	275:421	The aim of this study was to investigate the chemical composition and the antiinflammatory/antinociceptive properties of the hydroalcoholic extract derived from the leaves of Phyllanthus brasiliensis (HEPB) in rodents.
29670036	3	39	theme	extract	345:351	arg1	composition					259:269	the chemical composition	246:269	the chemical composition	246:269	The aim of this study was to investigate the chemical composition and the antiinflammatory/antinociceptive properties of the hydroalcoholic extract derived from the leaves of Phyllanthus brasiliensis (HEPB) in rodents.
29670036	7	40	from	models	881:886	arg1	rats					891:894	rats	891:894	rats	891:894	The anti-inflammatory effects of HEPB were assessed using carrageenan- and dextran-induced paw edema models in rats.
29670036	4	41	attach	isolated	538:545	arg2	B					497:497	5-O-β-d-glucopyranosyljusticidin B	464:497	5-O-β-d-glucopyranosyljusticidin B	464:497	A new arylnaphthalene lignan glycoside, 5-O-β-d-glucopyranosyljusticidin B, together with six known lignans, were isolated from HEPB.
29670036	4	41	attach	isolated	538:545	arg2	glycoside					453:461	A new arylnaphthalene lignan glycoside	424:461	A new arylnaphthalene lignan glycoside	424:461	A new arylnaphthalene lignan glycoside, 5-O-β-d-glucopyranosyljusticidin B, together with six known lignans, were isolated from HEPB.
29670036	4	41	attach	isolated	538:545	arg1	HEPB					552:555	HEPB	552:555	HEPB	552:555	A new arylnaphthalene lignan glycoside, 5-O-β-d-glucopyranosyljusticidin B, together with six known lignans, were isolated from HEPB.
29670036	4	42	theme	lignan	446:451	arg1	glycoside					453:461	A new arylnaphthalene lignan glycoside	424:461	A new arylnaphthalene lignan glycoside	424:461	A new arylnaphthalene lignan glycoside, 5-O-β-d-glucopyranosyljusticidin B, together with six known lignans, were isolated from HEPB.
29670036	4	42	theme	lignan	446:451	arg1	B					497:497	5-O-β-d-glucopyranosyljusticidin B	464:497	5-O-β-d-glucopyranosyljusticidin B	464:497	A new arylnaphthalene lignan glycoside, 5-O-β-d-glucopyranosyljusticidin B, together with six known lignans, were isolated from HEPB.
29670036	0	43	dep	Studies	38:44	arg1	Aubl					117:120	Aubl	117:120	Aubl.	117:121	Anti-Inflammatory and Antinociceptive Studies of Hydroalcoholic Extract from the Leaves of Phyllanthus brasiliensis (Aubl.)
29670036	10	44	theme	formalin-induced	1114:1129	arg1	phases					1104:1109	the neurogenic and inflammatory phases	1072:1109	the neurogenic and inflammatory phases of formalin-induced linking	1072:1137	HEPB and its fractions from FR6 to FR10 (FR6-10) inhibited the neurogenic and inflammatory phases of formalin-induced linking, demonstrating its antinociceptive activity.
29670036	6	45	theme	antinociceptive	669:683	arg1	activity					685:692	antinociceptive activity	669:692	antinociceptive activity	669:692	HEPB toxicity and antinociceptive activity were evaluated through acute oral toxicity and formalin models in mice, respectively.
29670036	9	46	theme	HEPB	946:949	arg1	administration					928:941	Oral administration	923:941	Oral administration of HEPB	923:949	Oral administration of HEPB reduced paw edema induced by carrageenan, but not by dextran.
29670036	4	47	theme	5-O-β-d-glucopyranosyljusticidin	464:495	arg1	glycoside					453:461	A new arylnaphthalene lignan glycoside	424:461	A new arylnaphthalene lignan glycoside	424:461	A new arylnaphthalene lignan glycoside, 5-O-β-d-glucopyranosyljusticidin B, together with six known lignans, were isolated from HEPB.
29670036	4	47	theme	5-O-β-d-glucopyranosyljusticidin	464:495	arg1	B					497:497	5-O-β-d-glucopyranosyljusticidin B	464:497	5-O-β-d-glucopyranosyljusticidin B	464:497	A new arylnaphthalene lignan glycoside, 5-O-β-d-glucopyranosyljusticidin B, together with six known lignans, were isolated from HEPB.
29670036	11	48	theme	antinociceptive/anti-inflammatory	1259:1291	arg1	effects					1293:1299	antinociceptive/anti-inflammatory effects	1259:1299	antinociceptive/anti-inflammatory effects not related to the histaminergic pathway	1259:1340	These results indicated that lignans from Phyllanthus brasiliensis exerted antinociceptive/anti-inflammatory effects not related to the histaminergic pathway.
29670036	7	49	theme	anti-inflammatory	784:800	arg1	effects					802:808	The anti-inflammatory effects	780:808	The anti-inflammatory effects of HEPB	780:816	The anti-inflammatory effects of HEPB were assessed using carrageenan- and dextran-induced paw edema models in rats.
29670036	10	50	from	FR6	1041:1043	arg1	HEPB					1013:1016	HEPB	1013:1016	HEPB	1013:1016	HEPB and its fractions from FR6 to FR10 (FR6-10) inhibited the neurogenic and inflammatory phases of formalin-induced linking, demonstrating its antinociceptive activity.
29670036	10	50	from	FR6	1041:1043	arg1	fractions					1026:1034	its fractions	1022:1034	its fractions from FR6 to FR10 (FR6-10)	1022:1060	HEPB and its fractions from FR6 to FR10 (FR6-10) inhibited the neurogenic and inflammatory phases of formalin-induced linking, demonstrating its antinociceptive activity.
29670036	5	51	theme	1D	558:559	arg1	experiments					572:582	1D and 2D NMR experiments	558:582	1D and 2D NMR experiments	558:582	1D and 2D NMR experiments and HRMS were used to elucidate the structure of the new compound.
29670036	8	52	theme	low	909:911	arg1	toxicity					913:920	low toxicity	909:920	low toxicity	909:920	HEPB showed low toxicity.
29670036	11	53	from	brasiliensis	1238:1249	arg1	lignans					1213:1219	lignans	1213:1219	lignans from Phyllanthus brasiliensis	1213:1249	These results indicated that lignans from Phyllanthus brasiliensis exerted antinociceptive/anti-inflammatory effects not related to the histaminergic pathway.
29670036	9	54	theme	paw	959:961	arg1	edema					963:967	paw edema	959:967	paw edema induced by carrageenan, but not by dextran	959:1010	Oral administration of HEPB reduced paw edema induced by carrageenan, but not by dextran.
29670036	0	55	from	Studies	38:44	arg1	the					77:79	the	77:79	the	77:79	Anti-Inflammatory and Antinociceptive Studies of Hydroalcoholic Extract from the Leaves of Phyllanthus brasiliensis (Aubl.)
29670036	6	56	from	models	750:755	arg1	mice					760:763	mice	760:763	mice	760:763	HEPB toxicity and antinociceptive activity were evaluated through acute oral toxicity and formalin models in mice, respectively.
29670036	6	57	theme	HEPB	651:654	arg1	toxicity					656:663	HEPB toxicity	651:663	HEPB toxicity	651:663	HEPB toxicity and antinociceptive activity were evaluated through acute oral toxicity and formalin models in mice, respectively.
29670036	11	58	theme	histaminergic	1320:1332	arg1	pathway					1334:1340	the histaminergic pathway	1316:1340	the histaminergic pathway	1316:1340	These results indicated that lignans from Phyllanthus brasiliensis exerted antinociceptive/anti-inflammatory effects not related to the histaminergic pathway.
30556801	9	0	theme	%	1136:1136	arg1	similarity					1117:1126	the highest 16S rRNA gene sequence similarity	1082:1126	the highest 16S rRNA gene sequence similarity of 98.3 % to its closest neighbour	1082:1161	In the phylogenetic tree based on 16S rRNA gene sequences, strain CPCC 204604T formed a distinct clade with Aeromicrobium ponti DSM 19178T within the lineage of the genus Aeromicrobium, with the highest 16S rRNA gene sequence similarity of 98.3 % to its closest neighbour.
30556801	4	1	theme	diagnostic	531:540	arg1	acid					550:553	the diagnostic diamino acid	527:553	the diagnostic diamino acid in cell-wall peptidoglycan	527:580	Polyphasic taxonomic analysis indicated that galactose and ribose were detected as the diagnostic sugars in the whole-cell hydrolysates, and ll-diaminopimelic acid was the diagnostic diamino acid in cell-wall peptidoglycan.
30556801	4	1	theme	diagnostic	531:540	arg1	acid					518:521	ll-diaminopimelic acid	500:521	ll-diaminopimelic acid	500:521	Polyphasic taxonomic analysis indicated that galactose and ribose were detected as the diagnostic sugars in the whole-cell hydrolysates, and ll-diaminopimelic acid was the diagnostic diamino acid in cell-wall peptidoglycan.
30556801	7	2	dep	C16 	784:787	arg1	C18 					807:810	C18 	807:810	C18 	807:810	The major fatty acids were C16 : 0, C16 : 0 2-OH, C18 : 1ω9c, C18 : 0 and 10-methyl C18:0.
30556801	7	2	dep	C16 	784:787	arg1	C16 					793:796	C16 	793:796	C16 	793:796	The major fatty acids were C16 : 0, C16 : 0 2-OH, C18 : 1ω9c, C18 : 0 and 10-methyl C18:0.
30556801	7	2	dep	C16 	784:787	arg1	 0					824:825	 0	824:825	 0	824:825	The major fatty acids were C16 : 0, C16 : 0 2-OH, C18 : 1ω9c, C18 : 0 and 10-methyl C18:0.
30556801	7	2	dep	C16 	784:787	arg1	C18:0					841:845	C18:0	841:845	C18:0	841:845	The major fatty acids were C16 : 0, C16 : 0 2-OH, C18 : 1ω9c, C18 : 0 and 10-methyl C18:0.
30556801	7	2	dep	C16 	784:787	arg1	C18 					819:822	C18 	819:822	C18 	819:822	The major fatty acids were C16 : 0, C16 : 0 2-OH, C18 : 1ω9c, C18 : 0 and 10-methyl C18:0.
30556801	5	3	theme	predominant	600:610	arg1	menaquinone					612:622	the predominant menaquinone	596:622	the predominant menaquinone	596:622	MK-9(H4) was the predominant menaquinone.
30556801	5	3	theme	predominant	600:610	arg1	MK-9					583:586	MK-9	583:586	MK-9(H4)	583:590	MK-9(H4) was the predominant menaquinone.
30556801	8	4	theme	genomic	852:858	arg1	G+C content					864:874	The genomic DNA G+C content	848:874	The genomic DNA G+C content	848:874	The genomic DNA G+C content was 71.2 mol%.
30556801	8	4	theme	genomic	852:858	arg1	%					888:888	71.2 mol%	880:888	71.2 mol%	880:888	The genomic DNA G+C content was 71.2 mol%.
30556801	7	5	theme	major	761:765	arg1	C16 					784:787	C16 	784:787	C16 	784:787	The major fatty acids were C16 : 0, C16 : 0 2-OH, C18 : 1ω9c, C18 : 0 and 10-methyl C18:0.
30556801	7	5	theme	major	761:765	arg1	acids					773:777	The major fatty acids	757:777	The major fatty acids	757:777	The major fatty acids were C16 : 0, C16 : 0 2-OH, C18 : 1ω9c, C18 : 0 and 10-methyl C18:0.
30556801	9	6	theme	16S	925:927	arg1	sequences					939:947	16S rRNA gene sequences	925:947	16S rRNA gene sequences	925:947	In the phylogenetic tree based on 16S rRNA gene sequences, strain CPCC 204604T formed a distinct clade with Aeromicrobium ponti DSM 19178T within the lineage of the genus Aeromicrobium, with the highest 16S rRNA gene sequence similarity of 98.3 % to its closest neighbour.
30556801	2	7	theme	actinobacterial	99:113	arg1	strain					115:120	An actinobacterial strain	96:120	An actinobacterial strain	96:120	An actinobacterial strain, designated CPCC 204604T, was isolated from a water sample collected from Sancha Lake, a drinking-water reservoir in Sichuan Province, south-west China.
30556801	10	8	theme	name	1321:1324	arg1	sp					1345:1346	the name Aeromicrobiumlacus sp	1317:1346	the name Aeromicrobiumlacus sp	1317:1346	On the basis of the genotypic and phenotypic data, we conclude that strain CPCC 204604T represents a novel species of the genus Aeromicrobium, for which the name Aeromicrobiumlacus sp.
30556801	10	9	theme	Aeromicrobium	1292:1304	arg1	species					1271:1277	a novel species	1263:1277	a novel species	1263:1277	On the basis of the genotypic and phenotypic data, we conclude that strain CPCC 204604T represents a novel species of the genus Aeromicrobium, for which the name Aeromicrobiumlacus sp.
30556801	9	10	theme	rRNA	929:932	arg1	sequences					939:947	16S rRNA gene sequences	925:947	16S rRNA gene sequences	925:947	In the phylogenetic tree based on 16S rRNA gene sequences, strain CPCC 204604T formed a distinct clade with Aeromicrobium ponti DSM 19178T within the lineage of the genus Aeromicrobium, with the highest 16S rRNA gene sequence similarity of 98.3 % to its closest neighbour.
30556801	9	11	theme	closest	1145:1151	arg1	neighbour					1153:1161	its closest neighbour	1141:1161	its closest neighbour	1141:1161	In the phylogenetic tree based on 16S rRNA gene sequences, strain CPCC 204604T formed a distinct clade with Aeromicrobium ponti DSM 19178T within the lineage of the genus Aeromicrobium, with the highest 16S rRNA gene sequence similarity of 98.3 % to its closest neighbour.
30556801	9	12	with	clade	988:992	arg1	19178T					1023:1028	Aeromicrobium ponti DSM 19178T	999:1028	Aeromicrobium ponti DSM 19178T	999:1028	In the phylogenetic tree based on 16S rRNA gene sequences, strain CPCC 204604T formed a distinct clade with Aeromicrobium ponti DSM 19178T within the lineage of the genus Aeromicrobium, with the highest 16S rRNA gene sequence similarity of 98.3 % to its closest neighbour.
30556801	10	13	theme	CPCC	1239:1242	arg1	204604T					1244:1250	strain CPCC 204604T	1232:1250	strain CPCC 204604T	1232:1250	On the basis of the genotypic and phenotypic data, we conclude that strain CPCC 204604T represents a novel species of the genus Aeromicrobium, for which the name Aeromicrobiumlacus sp.
30556801	11	14	theme	strain	1371:1376	arg1	204604T					1383:1389	strain CPCC 204604T	1371:1389	strain CPCC 204604T (=NBRC 112936T=DSM 105424T)	1371:1417	nov. is proposed with strain CPCC 204604T (=NBRC 112936T=DSM 105424T) as the type strain.
30556801	11	14	theme	strain	1371:1376	arg1	105424T					1410:1416	=NBRC 112936T=DSM 105424T	1392:1416	=NBRC 112936T=DSM 105424T	1392:1416	nov. is proposed with strain CPCC 204604T (=NBRC 112936T=DSM 105424T) as the type strain.
30556801	9	15	theme	gene	934:937	arg1	sequences					939:947	16S rRNA gene sequences	925:947	16S rRNA gene sequences	925:947	In the phylogenetic tree based on 16S rRNA gene sequences, strain CPCC 204604T formed a distinct clade with Aeromicrobium ponti DSM 19178T within the lineage of the genus Aeromicrobium, with the highest 16S rRNA gene sequence similarity of 98.3 % to its closest neighbour.
30556801	11	16	theme	CPCC	1378:1381	arg1	204604T					1383:1389	strain CPCC 204604T	1371:1389	strain CPCC 204604T (=NBRC 112936T=DSM 105424T)	1371:1417	nov. is proposed with strain CPCC 204604T (=NBRC 112936T=DSM 105424T) as the type strain.
30556801	11	16	theme	CPCC	1378:1381	arg1	105424T					1410:1416	=NBRC 112936T=DSM 105424T	1392:1416	=NBRC 112936T=DSM 105424T	1392:1416	nov. is proposed with strain CPCC 204604T (=NBRC 112936T=DSM 105424T) as the type strain.
30556801	2	17	theme	south-west	257:266	arg1	China					268:272	south-west China	257:272	south-west China	257:272	An actinobacterial strain, designated CPCC 204604T, was isolated from a water sample collected from Sancha Lake, a drinking-water reservoir in Sichuan Province, south-west China.
30556801	2	17	theme	south-west	257:266	arg1	Province					247:254	Sichuan Province	239:254	Sichuan Province	239:254	An actinobacterial strain, designated CPCC 204604T, was isolated from a water sample collected from Sancha Lake, a drinking-water reservoir in Sichuan Province, south-west China.
30556801	8	18	theme	DNA	860:862	arg1	G+C content					864:874	The genomic DNA G+C content	848:874	The genomic DNA G+C content	848:874	The genomic DNA G+C content was 71.2 mol%.
30556801	8	18	theme	DNA	860:862	arg1	%					888:888	71.2 mol%	880:888	71.2 mol%	880:888	The genomic DNA G+C content was 71.2 mol%.
30556801	4	19	theme	ll-diaminopimelic	500:516	arg1	acid					550:553	the diagnostic diamino acid	527:553	the diagnostic diamino acid in cell-wall peptidoglycan	527:580	Polyphasic taxonomic analysis indicated that galactose and ribose were detected as the diagnostic sugars in the whole-cell hydrolysates, and ll-diaminopimelic acid was the diagnostic diamino acid in cell-wall peptidoglycan.
30556801	4	19	theme	ll-diaminopimelic	500:516	arg1	acid					518:521	ll-diaminopimelic acid	500:521	ll-diaminopimelic acid	500:521	Polyphasic taxonomic analysis indicated that galactose and ribose were detected as the diagnostic sugars in the whole-cell hydrolysates, and ll-diaminopimelic acid was the diagnostic diamino acid in cell-wall peptidoglycan.
30556801	9	20	theme	strain	950:955	arg1	204604T					962:968	strain CPCC 204604T	950:968	strain CPCC 204604T	950:968	In the phylogenetic tree based on 16S rRNA gene sequences, strain CPCC 204604T formed a distinct clade with Aeromicrobium ponti DSM 19178T within the lineage of the genus Aeromicrobium, with the highest 16S rRNA gene sequence similarity of 98.3 % to its closest neighbour.
30556801	8	21	theme	71.2 mol	880:887	arg1	G+C content					864:874	The genomic DNA G+C content	848:874	The genomic DNA G+C content	848:874	The genomic DNA G+C content was 71.2 mol%.
30556801	8	21	theme	71.2 mol	880:887	arg1	%					888:888	71.2 mol%	880:888	71.2 mol%	880:888	The genomic DNA G+C content was 71.2 mol%.
30556801	0	22	theme	lacus	14:18	arg1	sp					20:21	Aeromicrobium lacus sp	0:21	Aeromicrobium lacus sp.	0:22	Aeromicrobium lacus sp.
30556801	2	23	theme	CPCC	134:137	arg1	204604T					139:145	CPCC 204604T	134:145	CPCC 204604T	134:145	An actinobacterial strain, designated CPCC 204604T, was isolated from a water sample collected from Sancha Lake, a drinking-water reservoir in Sichuan Province, south-west China.
30556801	4	24	theme	taxonomic	370:378	arg1	analysis					380:387	Polyphasic taxonomic analysis	359:387	Polyphasic taxonomic analysis	359:387	Polyphasic taxonomic analysis indicated that galactose and ribose were detected as the diagnostic sugars in the whole-cell hydrolysates, and ll-diaminopimelic acid was the diagnostic diamino acid in cell-wall peptidoglycan.
30556801	1	25	theme	drinking-water	70:83	arg1	reservoir					85:93	a drinking-water reservoir	68:93	a drinking-water reservoir	68:93	nov., a novel actinobacterium isolated from a drinking-water reservoir.
30556801	9	26	theme	Aeromicrobium	1062:1074	arg1	lineage					1041:1047	the lineage	1037:1047	the lineage of the genus Aeromicrobium	1037:1074	In the phylogenetic tree based on 16S rRNA gene sequences, strain CPCC 204604T formed a distinct clade with Aeromicrobium ponti DSM 19178T within the lineage of the genus Aeromicrobium, with the highest 16S rRNA gene sequence similarity of 98.3 % to its closest neighbour.
30556801	0	27	theme	Aeromicrobium	0:12	arg1	sp					20:21	Aeromicrobium lacus sp	0:21	Aeromicrobium lacus sp.	0:22	Aeromicrobium lacus sp.
30556801	10	28	theme	novel	1265:1269	arg1	species					1271:1277	a novel species	1263:1277	a novel species	1263:1277	On the basis of the genotypic and phenotypic data, we conclude that strain CPCC 204604T represents a novel species of the genus Aeromicrobium, for which the name Aeromicrobiumlacus sp.
30556801	9	29	theme	CPCC	957:960	arg1	204604T					962:968	strain CPCC 204604T	950:968	strain CPCC 204604T	950:968	In the phylogenetic tree based on 16S rRNA gene sequences, strain CPCC 204604T formed a distinct clade with Aeromicrobium ponti DSM 19178T within the lineage of the genus Aeromicrobium, with the highest 16S rRNA gene sequence similarity of 98.3 % to its closest neighbour.
30556801	10	30	theme	genus	1286:1290	arg1	Aeromicrobium					1292:1304	the genus Aeromicrobium	1282:1304	the genus Aeromicrobium	1282:1304	On the basis of the genotypic and phenotypic data, we conclude that strain CPCC 204604T represents a novel species of the genus Aeromicrobium, for which the name Aeromicrobiumlacus sp.
30556801	2	31	from	reservoir	226:234	arg1	China					268:272	south-west China	257:272	south-west China	257:272	An actinobacterial strain, designated CPCC 204604T, was isolated from a water sample collected from Sancha Lake, a drinking-water reservoir in Sichuan Province, south-west China.
30556801	2	31	from	reservoir	226:234	arg1	Province					247:254	Sichuan Province	239:254	Sichuan Province	239:254	An actinobacterial strain, designated CPCC 204604T, was isolated from a water sample collected from Sancha Lake, a drinking-water reservoir in Sichuan Province, south-west China.
30556801	10	32	theme	genotypic	1184:1192	arg1	data					1209:1212	the genotypic and phenotypic data	1180:1212	the genotypic and phenotypic data	1180:1212	On the basis of the genotypic and phenotypic data, we conclude that strain CPCC 204604T represents a novel species of the genus Aeromicrobium, for which the name Aeromicrobiumlacus sp.
30556801	11	33	theme	type	1426:1429	arg1	nov.					1349:1352	nov.	1349:1352	nov.	1349:1352	nov. is proposed with strain CPCC 204604T (=NBRC 112936T=DSM 105424T) as the type strain.
30556801	11	33	theme	type	1426:1429	arg1	strain					1431:1436	the type strain	1422:1436	the type strain	1422:1436	nov. is proposed with strain CPCC 204604T (=NBRC 112936T=DSM 105424T) as the type strain.
30556801	9	34	theme	gene	1103:1106	arg1	similarity					1117:1126	the highest 16S rRNA gene sequence similarity	1082:1126	the highest 16S rRNA gene sequence similarity of 98.3 % to its closest neighbour	1082:1161	In the phylogenetic tree based on 16S rRNA gene sequences, strain CPCC 204604T formed a distinct clade with Aeromicrobium ponti DSM 19178T within the lineage of the genus Aeromicrobium, with the highest 16S rRNA gene sequence similarity of 98.3 % to its closest neighbour.
30556801	2	35	theme	water	168:172	arg1	sample					174:179	a water sample	166:179	a water sample collected from Sancha Lake, a drinking-water reservoir in Sichuan Province, south-west China	166:272	An actinobacterial strain, designated CPCC 204604T, was isolated from a water sample collected from Sancha Lake, a drinking-water reservoir in Sichuan Province, south-west China.
30556801	11	36	theme	=NBRC	1392:1396	arg1	204604T					1383:1389	strain CPCC 204604T	1371:1389	strain CPCC 204604T (=NBRC 112936T=DSM 105424T)	1371:1417	nov. is proposed with strain CPCC 204604T (=NBRC 112936T=DSM 105424T) as the type strain.
30556801	11	36	theme	=NBRC	1392:1396	arg1	105424T					1410:1416	=NBRC 112936T=DSM 105424T	1392:1416	=NBRC 112936T=DSM 105424T	1392:1416	nov. is proposed with strain CPCC 204604T (=NBRC 112936T=DSM 105424T) as the type strain.
30556801	11	37	theme	112936T=DSM	1398:1408	arg1	204604T					1383:1389	strain CPCC 204604T	1371:1389	strain CPCC 204604T (=NBRC 112936T=DSM 105424T)	1371:1417	nov. is proposed with strain CPCC 204604T (=NBRC 112936T=DSM 105424T) as the type strain.
30556801	11	37	theme	112936T=DSM	1398:1408	arg1	105424T					1410:1416	=NBRC 112936T=DSM 105424T	1392:1416	=NBRC 112936T=DSM 105424T	1392:1416	nov. is proposed with strain CPCC 204604T (=NBRC 112936T=DSM 105424T) as the type strain.
30556801	10	38	theme	strain	1232:1237	arg1	204604T					1244:1250	strain CPCC 204604T	1232:1250	strain CPCC 204604T	1232:1250	On the basis of the genotypic and phenotypic data, we conclude that strain CPCC 204604T represents a novel species of the genus Aeromicrobium, for which the name Aeromicrobiumlacus sp.
30556801	7	39	theme	fatty	767:771	arg1	C16 					784:787	C16 	784:787	C16 	784:787	The major fatty acids were C16 : 0, C16 : 0 2-OH, C18 : 1ω9c, C18 : 0 and 10-methyl C18:0.
30556801	7	39	theme	fatty	767:771	arg1	acids					773:777	The major fatty acids	757:777	The major fatty acids	757:777	The major fatty acids were C16 : 0, C16 : 0 2-OH, C18 : 1ω9c, C18 : 0 and 10-methyl C18:0.
30556801	2	40	theme	drinking-water	211:224	arg1	Lake					203:206	Lake	203:206	Lake	203:206	An actinobacterial strain, designated CPCC 204604T, was isolated from a water sample collected from Sancha Lake, a drinking-water reservoir in Sichuan Province, south-west China.
30556801	2	40	theme	drinking-water	211:224	arg1	reservoir					226:234	a drinking-water reservoir	209:234	a drinking-water reservoir in Sichuan Province, south-west China	209:272	An actinobacterial strain, designated CPCC 204604T, was isolated from a water sample collected from Sancha Lake, a drinking-water reservoir in Sichuan Province, south-west China.
30556801	9	41	theme	highest	1086:1092	arg1	similarity					1117:1126	the highest 16S rRNA gene sequence similarity	1082:1126	the highest 16S rRNA gene sequence similarity of 98.3 % to its closest neighbour	1082:1161	In the phylogenetic tree based on 16S rRNA gene sequences, strain CPCC 204604T formed a distinct clade with Aeromicrobium ponti DSM 19178T within the lineage of the genus Aeromicrobium, with the highest 16S rRNA gene sequence similarity of 98.3 % to its closest neighbour.
30556801	2	42	attach	isolated	152:159	arg1	sample					174:179	a water sample	166:179	a water sample collected from Sancha Lake, a drinking-water reservoir in Sichuan Province, south-west China	166:272	An actinobacterial strain, designated CPCC 204604T, was isolated from a water sample collected from Sancha Lake, a drinking-water reservoir in Sichuan Province, south-west China.
30556801	2	42	attach	isolated	152:159	arg2	strain					115:120	An actinobacterial strain	96:120	An actinobacterial strain	96:120	An actinobacterial strain, designated CPCC 204604T, was isolated from a water sample collected from Sancha Lake, a drinking-water reservoir in Sichuan Province, south-west China.
30556801	4	43	from	sugars	457:462	arg1	hydrolysates					482:493	the whole-cell hydrolysates	467:493	the whole-cell hydrolysates	467:493	Polyphasic taxonomic analysis indicated that galactose and ribose were detected as the diagnostic sugars in the whole-cell hydrolysates, and ll-diaminopimelic acid was the diagnostic diamino acid in cell-wall peptidoglycan.
30556801	4	44	theme	whole-cell	471:480	arg1	hydrolysates					482:493	the whole-cell hydrolysates	467:493	the whole-cell hydrolysates	467:493	Polyphasic taxonomic analysis indicated that galactose and ribose were detected as the diagnostic sugars in the whole-cell hydrolysates, and ll-diaminopimelic acid was the diagnostic diamino acid in cell-wall peptidoglycan.
30556801	9	45	theme	16S	1094:1096	arg1	similarity					1117:1126	the highest 16S rRNA gene sequence similarity	1082:1126	the highest 16S rRNA gene sequence similarity of 98.3 % to its closest neighbour	1082:1161	In the phylogenetic tree based on 16S rRNA gene sequences, strain CPCC 204604T formed a distinct clade with Aeromicrobium ponti DSM 19178T within the lineage of the genus Aeromicrobium, with the highest 16S rRNA gene sequence similarity of 98.3 % to its closest neighbour.
30556801	4	46	from	acid	550:553	arg1	peptidoglycan					568:580	cell-wall peptidoglycan	558:580	cell-wall peptidoglycan	558:580	Polyphasic taxonomic analysis indicated that galactose and ribose were detected as the diagnostic sugars in the whole-cell hydrolysates, and ll-diaminopimelic acid was the diagnostic diamino acid in cell-wall peptidoglycan.
30556801	4	47	theme	diamino	542:548	arg1	acid					550:553	the diagnostic diamino acid	527:553	the diagnostic diamino acid in cell-wall peptidoglycan	527:580	Polyphasic taxonomic analysis indicated that galactose and ribose were detected as the diagnostic sugars in the whole-cell hydrolysates, and ll-diaminopimelic acid was the diagnostic diamino acid in cell-wall peptidoglycan.
30556801	4	47	theme	diamino	542:548	arg1	acid					518:521	ll-diaminopimelic acid	500:521	ll-diaminopimelic acid	500:521	Polyphasic taxonomic analysis indicated that galactose and ribose were detected as the diagnostic sugars in the whole-cell hydrolysates, and ll-diaminopimelic acid was the diagnostic diamino acid in cell-wall peptidoglycan.
30556801	10	48	dep	data	1209:1212	arg1	the					1167:1169	the	1167:1169	the	1167:1169	On the basis of the genotypic and phenotypic data, we conclude that strain CPCC 204604T represents a novel species of the genus Aeromicrobium, for which the name Aeromicrobiumlacus sp.
30556801	10	48	dep	data	1209:1212	arg1	basis					1171:1175	basis	1171:1175	basis	1171:1175	On the basis of the genotypic and phenotypic data, we conclude that strain CPCC 204604T represents a novel species of the genus Aeromicrobium, for which the name Aeromicrobiumlacus sp.
30556801	10	49	theme	Aeromicrobiumlacus	1326:1343	arg1	sp					1345:1346	the name Aeromicrobiumlacus sp	1317:1346	the name Aeromicrobiumlacus sp	1317:1346	On the basis of the genotypic and phenotypic data, we conclude that strain CPCC 204604T represents a novel species of the genus Aeromicrobium, for which the name Aeromicrobiumlacus sp.
30556801	9	50	theme	rRNA	1098:1101	arg1	similarity					1117:1126	the highest 16S rRNA gene sequence similarity	1082:1126	the highest 16S rRNA gene sequence similarity of 98.3 % to its closest neighbour	1082:1161	In the phylogenetic tree based on 16S rRNA gene sequences, strain CPCC 204604T formed a distinct clade with Aeromicrobium ponti DSM 19178T within the lineage of the genus Aeromicrobium, with the highest 16S rRNA gene sequence similarity of 98.3 % to its closest neighbour.
30556801	4	51	theme	cell-wall	558:566	arg1	peptidoglycan					568:580	cell-wall peptidoglycan	558:580	cell-wall peptidoglycan	558:580	Polyphasic taxonomic analysis indicated that galactose and ribose were detected as the diagnostic sugars in the whole-cell hydrolysates, and ll-diaminopimelic acid was the diagnostic diamino acid in cell-wall peptidoglycan.
30556801	4	52	theme	Polyphasic	359:368	arg1	analysis					380:387	Polyphasic taxonomic analysis	359:387	Polyphasic taxonomic analysis	359:387	Polyphasic taxonomic analysis indicated that galactose and ribose were detected as the diagnostic sugars in the whole-cell hydrolysates, and ll-diaminopimelic acid was the diagnostic diamino acid in cell-wall peptidoglycan.
30556801	9	53	theme	sequence	1108:1115	arg1	similarity					1117:1126	the highest 16S rRNA gene sequence similarity	1082:1126	the highest 16S rRNA gene sequence similarity of 98.3 % to its closest neighbour	1082:1161	In the phylogenetic tree based on 16S rRNA gene sequences, strain CPCC 204604T formed a distinct clade with Aeromicrobium ponti DSM 19178T within the lineage of the genus Aeromicrobium, with the highest 16S rRNA gene sequence similarity of 98.3 % to its closest neighbour.
30556801	9	54	theme	Aeromicrobium	999:1011	arg1	19178T					1023:1028	Aeromicrobium ponti DSM 19178T	999:1028	Aeromicrobium ponti DSM 19178T	999:1028	In the phylogenetic tree based on 16S rRNA gene sequences, strain CPCC 204604T formed a distinct clade with Aeromicrobium ponti DSM 19178T within the lineage of the genus Aeromicrobium, with the highest 16S rRNA gene sequence similarity of 98.3 % to its closest neighbour.
30556801	4	55	theme	diagnostic	446:455	arg1	ribose					418:423	ribose	418:423	ribose	418:423	Polyphasic taxonomic analysis indicated that galactose and ribose were detected as the diagnostic sugars in the whole-cell hydrolysates, and ll-diaminopimelic acid was the diagnostic diamino acid in cell-wall peptidoglycan.
30556801	4	55	theme	diagnostic	446:455	arg1	galactose					404:412	galactose	404:412	galactose	404:412	Polyphasic taxonomic analysis indicated that galactose and ribose were detected as the diagnostic sugars in the whole-cell hydrolysates, and ll-diaminopimelic acid was the diagnostic diamino acid in cell-wall peptidoglycan.
30556801	4	55	theme	diagnostic	446:455	arg1	sugars					457:462	the diagnostic sugars	442:462	the diagnostic sugars in the whole-cell hydrolysates	442:493	Polyphasic taxonomic analysis indicated that galactose and ribose were detected as the diagnostic sugars in the whole-cell hydrolysates, and ll-diaminopimelic acid was the diagnostic diamino acid in cell-wall peptidoglycan.
30556801	2	56	theme	Sichuan	239:245	arg1	China					268:272	south-west China	257:272	south-west China	257:272	An actinobacterial strain, designated CPCC 204604T, was isolated from a water sample collected from Sancha Lake, a drinking-water reservoir in Sichuan Province, south-west China.
30556801	2	56	theme	Sichuan	239:245	arg1	Province					247:254	Sichuan Province	239:254	Sichuan Province	239:254	An actinobacterial strain, designated CPCC 204604T, was isolated from a water sample collected from Sancha Lake, a drinking-water reservoir in Sichuan Province, south-west China.
30556801	6	57	theme	unidentified	730:741	arg1	phospholipid					743:754	an unidentified phospholipid	727:754	an unidentified phospholipid	727:754	The phospholipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unidentified phospholipid.
30556801	9	58	theme	ponti	1013:1017	arg1	19178T					1023:1028	Aeromicrobium ponti DSM 19178T	999:1028	Aeromicrobium ponti DSM 19178T	999:1028	In the phylogenetic tree based on 16S rRNA gene sequences, strain CPCC 204604T formed a distinct clade with Aeromicrobium ponti DSM 19178T within the lineage of the genus Aeromicrobium, with the highest 16S rRNA gene sequence similarity of 98.3 % to its closest neighbour.
30556801	9	59	theme	genus	1056:1060	arg1	Aeromicrobium					1062:1074	the genus Aeromicrobium	1052:1074	the genus Aeromicrobium	1052:1074	In the phylogenetic tree based on 16S rRNA gene sequences, strain CPCC 204604T formed a distinct clade with Aeromicrobium ponti DSM 19178T within the lineage of the genus Aeromicrobium, with the highest 16S rRNA gene sequence similarity of 98.3 % to its closest neighbour.
30556801	9	60	theme	phylogenetic	898:909	arg1	tree					911:914	the phylogenetic tree	894:914	the phylogenetic tree based on 16S rRNA gene sequences	894:947	In the phylogenetic tree based on 16S rRNA gene sequences, strain CPCC 204604T formed a distinct clade with Aeromicrobium ponti DSM 19178T within the lineage of the genus Aeromicrobium, with the highest 16S rRNA gene sequence similarity of 98.3 % to its closest neighbour.
30556801	10	61	theme	phenotypic	1198:1207	arg1	data					1209:1212	the genotypic and phenotypic data	1180:1212	the genotypic and phenotypic data	1180:1212	On the basis of the genotypic and phenotypic data, we conclude that strain CPCC 204604T represents a novel species of the genus Aeromicrobium, for which the name Aeromicrobiumlacus sp.
30556801	9	62	theme	DSM	1019:1021	arg1	19178T					1023:1028	Aeromicrobium ponti DSM 19178T	999:1028	Aeromicrobium ponti DSM 19178T	999:1028	In the phylogenetic tree based on 16S rRNA gene sequences, strain CPCC 204604T formed a distinct clade with Aeromicrobium ponti DSM 19178T within the lineage of the genus Aeromicrobium, with the highest 16S rRNA gene sequence similarity of 98.3 % to its closest neighbour.
30556801	9	63	theme	distinct	979:986	arg1	clade					988:992	a distinct clade	977:992	a distinct clade with Aeromicrobium ponti DSM 19178T within the lineage of the genus Aeromicrobium	977:1074	In the phylogenetic tree based on 16S rRNA gene sequences, strain CPCC 204604T formed a distinct clade with Aeromicrobium ponti DSM 19178T within the lineage of the genus Aeromicrobium, with the highest 16S rRNA gene sequence similarity of 98.3 % to its closest neighbour.
30556801	1	64	theme	novel	32:36	arg1	actinobacterium					38:52	a novel actinobacterium	30:52	a novel actinobacterium	30:52	nov., a novel actinobacterium isolated from a drinking-water reservoir.
30556801	1	64	theme	novel	32:36	arg1	nov.					24:27	nov.	24:27	nov.	24:27	nov., a novel actinobacterium isolated from a drinking-water reservoir.
29492866	7	0	theme	respiratory	928:938	arg1	menaquinone-7					952:964	menaquinone-7	952:964	menaquinone-7	952:964	The predominant respiratory quinone was menaquinone-7 and the major fatty acids were iso-C15:0 and iso-C17:1ω10c.
29492866	7	0	theme	respiratory	928:938	arg1	quinone					940:946	The predominant respiratory quinone	912:946	The predominant respiratory quinone	912:946	The predominant respiratory quinone was menaquinone-7 and the major fatty acids were iso-C15:0 and iso-C17:1ω10c.
29492866	6	1	theme	mol	906:908	arg1	%					909:909	43.6 mol%	901:909	43.6 mol%	901:909	The G + C content of the genomic DNA was 43.6 mol%.
29492866	6	1	theme	mol	906:908	arg1	content					870:876	The G + C content	860:876	content	870:876	The G + C content of the genomic DNA was 43.6 mol%.
29492866	11	2	theme	=	1374:1374	arg1	135PIL107-10T					1359:1371	the type strain 135PIL107-10T	1343:1371	the type strain 135PIL107-10T (= KACC 19275T = LMG 30080T)	1343:1400	nov. is proposed, with the type strain 135PIL107-10T (= KACC 19275T = LMG 30080T).
29492866	11	2	theme	=	1374:1374	arg1	30080T					1394:1399	= KACC 19275T = LMG 30080T	1374:1399	= KACC 19275T = LMG 30080T	1374:1399	nov. is proposed, with the type strain 135PIL107-10T (= KACC 19275T = LMG 30080T).
29492866	4	3	theme	new	503:505	arg1	branch					507:512	a new branch	501:512	a new branch within the genus Bacillus of the family Bacillaceae	501:564	Based on 16S rRNA gene phylogeny analysis, the novel strain formed a new branch within the genus Bacillus of the family Bacillaceae, and formed clusters with Bacillus thaohiensis NHI-38T (96.8%), Bacillus fengqiuensis NPK15T (96.7%), and Bacillus songklensis CAU 1033T (96.7%).
29492866	4	4	theme	rRNA	447:450	arg1	analysis					467:474	16S rRNA gene phylogeny analysis	443:474	16S rRNA gene phylogeny analysis	443:474	Based on 16S rRNA gene phylogeny analysis, the novel strain formed a new branch within the genus Bacillus of the family Bacillaceae, and formed clusters with Bacillus thaohiensis NHI-38T (96.8%), Bacillus fengqiuensis NPK15T (96.7%), and Bacillus songklensis CAU 1033T (96.7%).
29492866	3	5	dep	temperature	356:366	arg1	25°C					369:372	25°C	369:372	25°C	369:372	Strain 135PIL107-10T grew at 20-37°C (optimum temperature, 25°C) and pH 6.0-10.0 (optimum pH, 6.0) on marine and R2A agars.
29492866	11	6	theme	=	1388:1388	arg1	135PIL107-10T					1359:1371	the type strain 135PIL107-10T	1343:1371	the type strain 135PIL107-10T (= KACC 19275T = LMG 30080T)	1343:1400	nov. is proposed, with the type strain 135PIL107-10T (= KACC 19275T = LMG 30080T).
29492866	11	6	theme	=	1388:1388	arg1	30080T					1394:1399	= KACC 19275T = LMG 30080T	1374:1399	= KACC 19275T = LMG 30080T	1374:1399	nov. is proposed, with the type strain 135PIL107-10T (= KACC 19275T = LMG 30080T).
29492866	10	7	theme	name	1293:1296	arg1	sp					1316:1317	name Bacillus spongiae sp	1293:1317	the name Bacillus spongiae sp	1289:1317	The isolate therefore represents a novel species, for which the name Bacillus spongiae sp.
29492866	11	8	theme	strain	1352:1357	arg1	135PIL107-10T					1359:1371	the type strain 135PIL107-10T	1343:1371	the type strain 135PIL107-10T (= KACC 19275T = LMG 30080T)	1343:1400	nov. is proposed, with the type strain 135PIL107-10T (= KACC 19275T = LMG 30080T).
29492866	11	8	theme	strain	1352:1357	arg1	30080T					1394:1399	= KACC 19275T = LMG 30080T	1374:1399	= KACC 19275T = LMG 30080T	1374:1399	nov. is proposed, with the type strain 135PIL107-10T (= KACC 19275T = LMG 30080T).
29492866	5	9	theme	Bacillus	826:833	arg1	members					805:811	all other recognized members	784:811	all other recognized members of the genus Bacillus (95.6-96.8% similarity)	784:857	Lower sequence similarities (97.0%) were found with the type strains of all other recognized members of the genus Bacillus (95.6-96.8% similarity).
29492866	2	10	dep	Gram-reaction-positive	67:88	arg1	aerobic					100:106	aerobic	100:106	aerobic	100:106	A Gram-reaction-positive, strictly aerobic, motile, endospore- forming, and rod-shaped bacterial strain designated 135PIL107-10T was isolated from a sponge on Jeju Island, and its taxonomic position was investigated using a polyphasic approach.
29492866	10	11	theme	Bacillus	1298:1305	arg1	sp					1316:1317	name Bacillus spongiae sp	1293:1317	the name Bacillus spongiae sp	1289:1317	The isolate therefore represents a novel species, for which the name Bacillus spongiae sp.
29492866	10	12	theme	novel	1264:1268	arg1	species					1270:1276	a novel species	1262:1276	a novel species	1262:1276	The isolate therefore represents a novel species, for which the name Bacillus spongiae sp.
29492866	6	13	theme	C	868:868	arg1	content					870:876	The G + C content	860:876	content	870:876	The G + C content of the genomic DNA was 43.6 mol%.
29492866	6	13	theme	C	868:868	arg1	%					909:909	43.6 mol%	901:909	43.6 mol%	901:909	The G + C content of the genomic DNA was 43.6 mol%.
29492866	9	14	theme	diamino	1155:1161	arg1	acid					1223:1226	meso-diaminopimelic acid	1203:1226	meso-diaminopimelic acid	1203:1226	The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid.
29492866	9	14	theme	diamino	1155:1161	arg1	acid					1163:1166	The diagnostic diamino acid	1140:1166	The diagnostic diamino acid in the cell-wall peptidoglycan	1140:1197	The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid.
29492866	7	15	theme	predominant	916:926	arg1	menaquinone-7					952:964	menaquinone-7	952:964	menaquinone-7	952:964	The predominant respiratory quinone was menaquinone-7 and the major fatty acids were iso-C15:0 and iso-C17:1ω10c.
29492866	7	15	theme	predominant	916:926	arg1	quinone					940:946	The predominant respiratory quinone	912:946	The predominant respiratory quinone	912:946	The predominant respiratory quinone was menaquinone-7 and the major fatty acids were iso-C15:0 and iso-C17:1ω10c.
29492866	11	16	theme	KACC	1376:1379	arg1	135PIL107-10T					1359:1371	the type strain 135PIL107-10T	1343:1371	the type strain 135PIL107-10T (= KACC 19275T = LMG 30080T)	1343:1400	nov. is proposed, with the type strain 135PIL107-10T (= KACC 19275T = LMG 30080T).
29492866	11	16	theme	KACC	1376:1379	arg1	30080T					1394:1399	= KACC 19275T = LMG 30080T	1374:1399	= KACC 19275T = LMG 30080T	1374:1399	nov. is proposed, with the type strain 135PIL107-10T (= KACC 19275T = LMG 30080T).
29492866	4	17	dep	Bacillus	630:637	arg1	fengqiuensis					639:650	fengqiuensis	639:650	fengqiuensis	639:650	Based on 16S rRNA gene phylogeny analysis, the novel strain formed a new branch within the genus Bacillus of the family Bacillaceae, and formed clusters with Bacillus thaohiensis NHI-38T (96.8%), Bacillus fengqiuensis NPK15T (96.7%), and Bacillus songklensis CAU 1033T (96.7%).
29492866	5	18	theme	Lower	712:716	arg1	similarities					727:738	Lower sequence similarities	712:738	Lower sequence similarities (97.0%)	712:746	Lower sequence similarities (97.0%) were found with the type strains of all other recognized members of the genus Bacillus (95.6-96.8% similarity).
29492866	5	18	theme	Lower	712:716	arg1	%					745:745	97.0%	741:745	97.0%	741:745	Lower sequence similarities (97.0%) were found with the type strains of all other recognized members of the genus Bacillus (95.6-96.8% similarity).
29492866	2	19	theme	forming	128:134	arg1	strain					162:167	A Gram-reaction-positive, strictly aerobic, motile, endospore- forming, and rod-shaped bacterial strain	65:167	A Gram-reaction-positive, strictly aerobic, motile, endospore- forming, and rod-shaped bacterial strain designated 135PIL107-10T	65:192	A Gram-reaction-positive, strictly aerobic, motile, endospore- forming, and rod-shaped bacterial strain designated 135PIL107-10T was isolated from a sponge on Jeju Island, and its taxonomic position was investigated using a polyphasic approach.
29492866	7	20	theme	fatty	980:984	arg1	iso-C15:0					997:1005	iso-C15:0	997:1005	iso-C15:0	997:1005	The predominant respiratory quinone was menaquinone-7 and the major fatty acids were iso-C15:0 and iso-C17:1ω10c.
29492866	7	20	theme	fatty	980:984	arg1	acids					986:990	the major fatty acids	970:990	the major fatty acids	970:990	The predominant respiratory quinone was menaquinone-7 and the major fatty acids were iso-C15:0 and iso-C17:1ω10c.
29492866	11	21	theme	19275T	1381:1386	arg1	135PIL107-10T					1359:1371	the type strain 135PIL107-10T	1343:1371	the type strain 135PIL107-10T (= KACC 19275T = LMG 30080T)	1343:1400	nov. is proposed, with the type strain 135PIL107-10T (= KACC 19275T = LMG 30080T).
29492866	11	21	theme	19275T	1381:1386	arg1	30080T					1394:1399	= KACC 19275T = LMG 30080T	1374:1399	= KACC 19275T = LMG 30080T	1374:1399	nov. is proposed, with the type strain 135PIL107-10T (= KACC 19275T = LMG 30080T).
29492866	1	22	theme	Jeju	52:55	arg1	Island					57:62	Jeju Island	52:62	Jeju Island	52:62	nov., isolated from sponge of Jeju Island.
29492866	6	23	theme	genomic	885:891	arg1	DNA					893:895	the genomic DNA	881:895	the genomic DNA	881:895	The G + C content of the genomic DNA was 43.6 mol%.
29492866	8	24	theme	polar	1038:1042	arg1	diphosphatidylglycerol					1064:1085	diphosphatidylglycerol	1064:1085	diphosphatidylglycerol	1064:1085	The overall polar lipid patterns were diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine.
29492866	8	24	theme	polar	1038:1042	arg1	patterns					1050:1057	The overall polar lipid patterns	1026:1057	The overall polar lipid patterns	1026:1057	The overall polar lipid patterns were diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine.
29492866	1	25	theme	Island	57:62	arg1	sponge					42:47	sponge	42:47	sponge of Jeju Island	42:62	nov., isolated from sponge of Jeju Island.
29492866	2	26	attach	isolated	198:205	arg1	sponge					214:219	a sponge	212:219	a sponge on Jeju Island	212:234	A Gram-reaction-positive, strictly aerobic, motile, endospore- forming, and rod-shaped bacterial strain designated 135PIL107-10T was isolated from a sponge on Jeju Island, and its taxonomic position was investigated using a polyphasic approach.
29492866	2	26	attach	isolated	198:205	arg2	strain					162:167	A Gram-reaction-positive, strictly aerobic, motile, endospore- forming, and rod-shaped bacterial strain	65:167	A Gram-reaction-positive, strictly aerobic, motile, endospore- forming, and rod-shaped bacterial strain designated 135PIL107-10T	65:192	A Gram-reaction-positive, strictly aerobic, motile, endospore- forming, and rod-shaped bacterial strain designated 135PIL107-10T was isolated from a sponge on Jeju Island, and its taxonomic position was investigated using a polyphasic approach.
29492866	0	27	theme	spongiae	9:16	arg1	sp					18:19	Bacillus spongiae sp	0:19	Bacillus spongiae sp.	0:20	Bacillus spongiae sp.
29492866	9	28	theme	cell-wall	1175:1183	arg1	peptidoglycan					1185:1197	the cell-wall peptidoglycan	1171:1197	the cell-wall peptidoglycan	1171:1197	The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid.
29492866	3	29	dep	pH	379:380	arg1	pH					400:401	optimum pH	392:401	optimum pH	392:401	Strain 135PIL107-10T grew at 20-37°C (optimum temperature, 25°C) and pH 6.0-10.0 (optimum pH, 6.0) on marine and R2A agars.
29492866	2	30	theme	polyphasic	289:298	arg1	approach					300:307	a polyphasic approach	287:307	a polyphasic approach	287:307	A Gram-reaction-positive, strictly aerobic, motile, endospore- forming, and rod-shaped bacterial strain designated 135PIL107-10T was isolated from a sponge on Jeju Island, and its taxonomic position was investigated using a polyphasic approach.
29492866	2	31	from	sponge	214:219	arg1	Island					229:234	Jeju Island	224:234	Jeju Island	224:234	A Gram-reaction-positive, strictly aerobic, motile, endospore- forming, and rod-shaped bacterial strain designated 135PIL107-10T was isolated from a sponge on Jeju Island, and its taxonomic position was investigated using a polyphasic approach.
29492866	4	32	dep	Bacillus	672:679	arg1	songklensis					681:691	songklensis	681:691	songklensis	681:691	Based on 16S rRNA gene phylogeny analysis, the novel strain formed a new branch within the genus Bacillus of the family Bacillaceae, and formed clusters with Bacillus thaohiensis NHI-38T (96.8%), Bacillus fengqiuensis NPK15T (96.7%), and Bacillus songklensis CAU 1033T (96.7%).
29492866	0	33	theme	Bacillus	0:7	arg1	sp					18:19	Bacillus spongiae sp	0:19	Bacillus spongiae sp.	0:20	Bacillus spongiae sp.
29492866	4	34	theme	novel	481:485	arg1	strain					487:492	the novel strain	477:492	the novel strain	477:492	Based on 16S rRNA gene phylogeny analysis, the novel strain formed a new branch within the genus Bacillus of the family Bacillaceae, and formed clusters with Bacillus thaohiensis NHI-38T (96.8%), Bacillus fengqiuensis NPK15T (96.7%), and Bacillus songklensis CAU 1033T (96.7%).
29492866	4	35	theme	Bacillus	630:637	arg1	NPK15T					652:657	Bacillus fengqiuensis NPK15T	630:657	Bacillus fengqiuensis NPK15T (96.7%)	630:665	Based on 16S rRNA gene phylogeny analysis, the novel strain formed a new branch within the genus Bacillus of the family Bacillaceae, and formed clusters with Bacillus thaohiensis NHI-38T (96.8%), Bacillus fengqiuensis NPK15T (96.7%), and Bacillus songklensis CAU 1033T (96.7%).
29492866	4	35	theme	Bacillus	630:637	arg1	%					664:664	96.7%	660:664	96.7%	660:664	Based on 16S rRNA gene phylogeny analysis, the novel strain formed a new branch within the genus Bacillus of the family Bacillaceae, and formed clusters with Bacillus thaohiensis NHI-38T (96.8%), Bacillus fengqiuensis NPK15T (96.7%), and Bacillus songklensis CAU 1033T (96.7%).
29492866	3	36	theme	optimum	392:398	arg1	pH					400:401	optimum pH	392:401	optimum pH	392:401	Strain 135PIL107-10T grew at 20-37°C (optimum temperature, 25°C) and pH 6.0-10.0 (optimum pH, 6.0) on marine and R2A agars.
29492866	3	37	theme	optimum	348:354	arg1	20-37°C					339:345	20-37°C	339:345	20-37°C (optimum temperature, 25°C)	339:373	Strain 135PIL107-10T grew at 20-37°C (optimum temperature, 25°C) and pH 6.0-10.0 (optimum pH, 6.0) on marine and R2A agars.
29492866	3	37	theme	optimum	348:354	arg1	temperature					356:366	optimum temperature	348:366	optimum temperature	348:366	Strain 135PIL107-10T grew at 20-37°C (optimum temperature, 25°C) and pH 6.0-10.0 (optimum pH, 6.0) on marine and R2A agars.
29492866	5	38	theme	%	845:845	arg1	similarity					847:856	95.6-96.8% similarity	836:856	95.6-96.8% similarity	836:856	Lower sequence similarities (97.0%) were found with the type strains of all other recognized members of the genus Bacillus (95.6-96.8% similarity).
29492866	5	38	theme	%	845:845	arg1	Bacillus					826:833	the genus Bacillus	816:833	the genus Bacillus (95.6-96.8% similarity)	816:857	Lower sequence similarities (97.0%) were found with the type strains of all other recognized members of the genus Bacillus (95.6-96.8% similarity).
29492866	4	39	with	clusters	578:585	arg1	%					626:626	96.8%	622:626	96.8%	622:626	Based on 16S rRNA gene phylogeny analysis, the novel strain formed a new branch within the genus Bacillus of the family Bacillaceae, and formed clusters with Bacillus thaohiensis NHI-38T (96.8%), Bacillus fengqiuensis NPK15T (96.7%), and Bacillus songklensis CAU 1033T (96.7%).
29492866	4	39	with	clusters	578:585	arg1	%					664:664	96.7%	660:664	96.7%	660:664	Based on 16S rRNA gene phylogeny analysis, the novel strain formed a new branch within the genus Bacillus of the family Bacillaceae, and formed clusters with Bacillus thaohiensis NHI-38T (96.8%), Bacillus fengqiuensis NPK15T (96.7%), and Bacillus songklensis CAU 1033T (96.7%).
29492866	4	39	with	clusters	578:585	arg1	1033T					697:701	Bacillus songklensis CAU 1033T	672:701	Bacillus songklensis CAU 1033T (96.7%)	672:709	Based on 16S rRNA gene phylogeny analysis, the novel strain formed a new branch within the genus Bacillus of the family Bacillaceae, and formed clusters with Bacillus thaohiensis NHI-38T (96.8%), Bacillus fengqiuensis NPK15T (96.7%), and Bacillus songklensis CAU 1033T (96.7%).
29492866	4	39	with	clusters	578:585	arg1	NHI-38T					613:619	Bacillus thaohiensis NHI-38T	592:619	Bacillus thaohiensis NHI-38T (96.8%)	592:627	Based on 16S rRNA gene phylogeny analysis, the novel strain formed a new branch within the genus Bacillus of the family Bacillaceae, and formed clusters with Bacillus thaohiensis NHI-38T (96.8%), Bacillus fengqiuensis NPK15T (96.7%), and Bacillus songklensis CAU 1033T (96.7%).
29492866	4	39	with	clusters	578:585	arg1	%					708:708	96.7%	704:708	96.7%	704:708	Based on 16S rRNA gene phylogeny analysis, the novel strain formed a new branch within the genus Bacillus of the family Bacillaceae, and formed clusters with Bacillus thaohiensis NHI-38T (96.8%), Bacillus fengqiuensis NPK15T (96.7%), and Bacillus songklensis CAU 1033T (96.7%).
29492866	4	39	with	clusters	578:585	arg1	NPK15T					652:657	Bacillus fengqiuensis NPK15T	630:657	Bacillus fengqiuensis NPK15T (96.7%)	630:665	Based on 16S rRNA gene phylogeny analysis, the novel strain formed a new branch within the genus Bacillus of the family Bacillaceae, and formed clusters with Bacillus thaohiensis NHI-38T (96.8%), Bacillus fengqiuensis NPK15T (96.7%), and Bacillus songklensis CAU 1033T (96.7%).
29492866	5	40	theme	recognized	794:803	arg1	members					805:811	all other recognized members	784:811	all other recognized members of the genus Bacillus (95.6-96.8% similarity)	784:857	Lower sequence similarities (97.0%) were found with the type strains of all other recognized members of the genus Bacillus (95.6-96.8% similarity).
29492866	4	41	theme	family	547:552	arg1	Bacillaceae					554:564	the family Bacillaceae	543:564	the family Bacillaceae	543:564	Based on 16S rRNA gene phylogeny analysis, the novel strain formed a new branch within the genus Bacillus of the family Bacillaceae, and formed clusters with Bacillus thaohiensis NHI-38T (96.8%), Bacillus fengqiuensis NPK15T (96.7%), and Bacillus songklensis CAU 1033T (96.7%).
29492866	5	42	theme	other	788:792	arg1	members					805:811	all other recognized members	784:811	all other recognized members of the genus Bacillus (95.6-96.8% similarity)	784:857	Lower sequence similarities (97.0%) were found with the type strains of all other recognized members of the genus Bacillus (95.6-96.8% similarity).
29492866	9	43	theme	meso-diaminopimelic	1203:1221	arg1	acid					1223:1226	meso-diaminopimelic acid	1203:1226	meso-diaminopimelic acid	1203:1226	The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid.
29492866	9	43	theme	meso-diaminopimelic	1203:1221	arg1	acid					1163:1166	The diagnostic diamino acid	1140:1166	The diagnostic diamino acid in the cell-wall peptidoglycan	1140:1197	The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid.
29492866	2	44	theme	taxonomic	245:253	arg1	position					255:262	its taxonomic position	241:262	its taxonomic position	241:262	A Gram-reaction-positive, strictly aerobic, motile, endospore- forming, and rod-shaped bacterial strain designated 135PIL107-10T was isolated from a sponge on Jeju Island, and its taxonomic position was investigated using a polyphasic approach.
29492866	5	45	theme	members	805:811	arg1	strains					773:779	the type strains	764:779	the type strains of all other recognized members of the genus Bacillus (95.6-96.8% similarity)	764:857	Lower sequence similarities (97.0%) were found with the type strains of all other recognized members of the genus Bacillus (95.6-96.8% similarity).
29492866	3	46	theme	marine	412:417	arg1	agars					427:431	marine and R2A agars	412:431	agars	427:431	Strain 135PIL107-10T grew at 20-37°C (optimum temperature, 25°C) and pH 6.0-10.0 (optimum pH, 6.0) on marine and R2A agars.
29492866	8	47	theme	overall	1030:1036	arg1	diphosphatidylglycerol					1064:1085	diphosphatidylglycerol	1064:1085	diphosphatidylglycerol	1064:1085	The overall polar lipid patterns were diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine.
29492866	8	47	theme	overall	1030:1036	arg1	patterns					1050:1057	The overall polar lipid patterns	1026:1057	The overall polar lipid patterns	1026:1057	The overall polar lipid patterns were diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine.
29492866	2	48	dep	forming	128:134	arg1	endospore-					117:126	endospore-	117:126	endospore-	117:126	A Gram-reaction-positive, strictly aerobic, motile, endospore- forming, and rod-shaped bacterial strain designated 135PIL107-10T was isolated from a sponge on Jeju Island, and its taxonomic position was investigated using a polyphasic approach.
29492866	2	49	theme	bacterial	152:160	arg1	strain					162:167	A Gram-reaction-positive, strictly aerobic, motile, endospore- forming, and rod-shaped bacterial strain	65:167	A Gram-reaction-positive, strictly aerobic, motile, endospore- forming, and rod-shaped bacterial strain designated 135PIL107-10T	65:192	A Gram-reaction-positive, strictly aerobic, motile, endospore- forming, and rod-shaped bacterial strain designated 135PIL107-10T was isolated from a sponge on Jeju Island, and its taxonomic position was investigated using a polyphasic approach.
29492866	3	50	theme	6.0	404:406	arg1	pH					400:401	optimum pH	392:401	optimum pH	392:401	Strain 135PIL107-10T grew at 20-37°C (optimum temperature, 25°C) and pH 6.0-10.0 (optimum pH, 6.0) on marine and R2A agars.
29492866	9	51	theme	diagnostic	1144:1153	arg1	acid					1223:1226	meso-diaminopimelic acid	1203:1226	meso-diaminopimelic acid	1203:1226	The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid.
29492866	9	51	theme	diagnostic	1144:1153	arg1	acid					1163:1166	The diagnostic diamino acid	1140:1166	The diagnostic diamino acid in the cell-wall peptidoglycan	1140:1197	The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid.
29492866	4	52	theme	phylogeny	457:465	arg1	analysis					467:474	16S rRNA gene phylogeny analysis	443:474	16S rRNA gene phylogeny analysis	443:474	Based on 16S rRNA gene phylogeny analysis, the novel strain formed a new branch within the genus Bacillus of the family Bacillaceae, and formed clusters with Bacillus thaohiensis NHI-38T (96.8%), Bacillus fengqiuensis NPK15T (96.7%), and Bacillus songklensis CAU 1033T (96.7%).
29492866	2	53	theme	rod-shaped	141:150	arg1	strain					162:167	A Gram-reaction-positive, strictly aerobic, motile, endospore- forming, and rod-shaped bacterial strain	65:167	A Gram-reaction-positive, strictly aerobic, motile, endospore- forming, and rod-shaped bacterial strain designated 135PIL107-10T	65:192	A Gram-reaction-positive, strictly aerobic, motile, endospore- forming, and rod-shaped bacterial strain designated 135PIL107-10T was isolated from a sponge on Jeju Island, and its taxonomic position was investigated using a polyphasic approach.
29492866	7	54	theme	major	974:978	arg1	iso-C15:0					997:1005	iso-C15:0	997:1005	iso-C15:0	997:1005	The predominant respiratory quinone was menaquinone-7 and the major fatty acids were iso-C15:0 and iso-C17:1ω10c.
29492866	7	54	theme	major	974:978	arg1	acids					986:990	the major fatty acids	970:990	the major fatty acids	970:990	The predominant respiratory quinone was menaquinone-7 and the major fatty acids were iso-C15:0 and iso-C17:1ω10c.
29492866	4	55	theme	Bacillaceae	554:564	arg1	branch					507:512	a new branch	501:512	a new branch within the genus Bacillus of the family Bacillaceae	501:564	Based on 16S rRNA gene phylogeny analysis, the novel strain formed a new branch within the genus Bacillus of the family Bacillaceae, and formed clusters with Bacillus thaohiensis NHI-38T (96.8%), Bacillus fengqiuensis NPK15T (96.7%), and Bacillus songklensis CAU 1033T (96.7%).
29492866	8	56	theme	lipid	1044:1048	arg1	diphosphatidylglycerol					1064:1085	diphosphatidylglycerol	1064:1085	diphosphatidylglycerol	1064:1085	The overall polar lipid patterns were diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine.
29492866	8	56	theme	lipid	1044:1048	arg1	patterns					1050:1057	The overall polar lipid patterns	1026:1057	The overall polar lipid patterns	1026:1057	The overall polar lipid patterns were diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine.
29492866	2	57	theme	Gram-reaction-positive	67:88	arg1	strain					162:167	A Gram-reaction-positive, strictly aerobic, motile, endospore- forming, and rod-shaped bacterial strain	65:167	A Gram-reaction-positive, strictly aerobic, motile, endospore- forming, and rod-shaped bacterial strain designated 135PIL107-10T	65:192	A Gram-reaction-positive, strictly aerobic, motile, endospore- forming, and rod-shaped bacterial strain designated 135PIL107-10T was isolated from a sponge on Jeju Island, and its taxonomic position was investigated using a polyphasic approach.
29492866	10	58	theme	spongiae	1307:1314	arg1	sp					1316:1317	name Bacillus spongiae sp	1293:1317	the name Bacillus spongiae sp	1289:1317	The isolate therefore represents a novel species, for which the name Bacillus spongiae sp.
29492866	4	59	theme	16S	443:445	arg1	analysis					467:474	16S rRNA gene phylogeny analysis	443:474	16S rRNA gene phylogeny analysis	443:474	Based on 16S rRNA gene phylogeny analysis, the novel strain formed a new branch within the genus Bacillus of the family Bacillaceae, and formed clusters with Bacillus thaohiensis NHI-38T (96.8%), Bacillus fengqiuensis NPK15T (96.7%), and Bacillus songklensis CAU 1033T (96.7%).
29492866	11	60	theme	LMG	1390:1392	arg1	135PIL107-10T					1359:1371	the type strain 135PIL107-10T	1343:1371	the type strain 135PIL107-10T (= KACC 19275T = LMG 30080T)	1343:1400	nov. is proposed, with the type strain 135PIL107-10T (= KACC 19275T = LMG 30080T).
29492866	11	60	theme	LMG	1390:1392	arg1	30080T					1394:1399	= KACC 19275T = LMG 30080T	1374:1399	= KACC 19275T = LMG 30080T	1374:1399	nov. is proposed, with the type strain 135PIL107-10T (= KACC 19275T = LMG 30080T).
29492866	5	61	theme	type	768:771	arg1	strains					773:779	the type strains	764:779	the type strains of all other recognized members of the genus Bacillus (95.6-96.8% similarity)	764:857	Lower sequence similarities (97.0%) were found with the type strains of all other recognized members of the genus Bacillus (95.6-96.8% similarity).
29492866	4	62	theme	Bacillus	672:679	arg1	1033T					697:701	Bacillus songklensis CAU 1033T	672:701	Bacillus songklensis CAU 1033T (96.7%)	672:709	Based on 16S rRNA gene phylogeny analysis, the novel strain formed a new branch within the genus Bacillus of the family Bacillaceae, and formed clusters with Bacillus thaohiensis NHI-38T (96.8%), Bacillus fengqiuensis NPK15T (96.7%), and Bacillus songklensis CAU 1033T (96.7%).
29492866	4	62	theme	Bacillus	672:679	arg1	%					708:708	96.7%	704:708	96.7%	704:708	Based on 16S rRNA gene phylogeny analysis, the novel strain formed a new branch within the genus Bacillus of the family Bacillaceae, and formed clusters with Bacillus thaohiensis NHI-38T (96.8%), Bacillus fengqiuensis NPK15T (96.7%), and Bacillus songklensis CAU 1033T (96.7%).
29492866	9	63	from	acid	1163:1166	arg1	peptidoglycan					1185:1197	the cell-wall peptidoglycan	1171:1197	the cell-wall peptidoglycan	1171:1197	The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid.
29492866	11	64	theme	type	1347:1350	arg1	135PIL107-10T					1359:1371	the type strain 135PIL107-10T	1343:1371	the type strain 135PIL107-10T (= KACC 19275T = LMG 30080T)	1343:1400	nov. is proposed, with the type strain 135PIL107-10T (= KACC 19275T = LMG 30080T).
29492866	11	64	theme	type	1347:1350	arg1	30080T					1394:1399	= KACC 19275T = LMG 30080T	1374:1399	= KACC 19275T = LMG 30080T	1374:1399	nov. is proposed, with the type strain 135PIL107-10T (= KACC 19275T = LMG 30080T).
29492866	6	65	theme	G	864:864	arg1	content					870:876	The G + C content	860:876	content	870:876	The G + C content of the genomic DNA was 43.6 mol%.
29492866	6	65	theme	G	864:864	arg1	%					909:909	43.6 mol%	901:909	43.6 mol%	901:909	The G + C content of the genomic DNA was 43.6 mol%.
29492866	4	66	dep	Bacillus	592:599	arg1	thaohiensis					601:611	thaohiensis	601:611	thaohiensis	601:611	Based on 16S rRNA gene phylogeny analysis, the novel strain formed a new branch within the genus Bacillus of the family Bacillaceae, and formed clusters with Bacillus thaohiensis NHI-38T (96.8%), Bacillus fengqiuensis NPK15T (96.7%), and Bacillus songklensis CAU 1033T (96.7%).
29492866	5	67	theme	95.6-96.8	836:844	arg1	%					845:845	%	845:845	%	845:845	Lower sequence similarities (97.0%) were found with the type strains of all other recognized members of the genus Bacillus (95.6-96.8% similarity).
29492866	3	68	theme	R2A	423:425	arg1	agars					427:431	marine and R2A agars	412:431	agars	427:431	Strain 135PIL107-10T grew at 20-37°C (optimum temperature, 25°C) and pH 6.0-10.0 (optimum pH, 6.0) on marine and R2A agars.
29492866	5	69	theme	sequence	718:725	arg1	similarities					727:738	Lower sequence similarities	712:738	Lower sequence similarities (97.0%)	712:746	Lower sequence similarities (97.0%) were found with the type strains of all other recognized members of the genus Bacillus (95.6-96.8% similarity).
29492866	5	69	theme	sequence	718:725	arg1	%					745:745	97.0%	741:745	97.0%	741:745	Lower sequence similarities (97.0%) were found with the type strains of all other recognized members of the genus Bacillus (95.6-96.8% similarity).
29492866	4	70	theme	CAU	693:695	arg1	1033T					697:701	Bacillus songklensis CAU 1033T	672:701	Bacillus songklensis CAU 1033T (96.7%)	672:709	Based on 16S rRNA gene phylogeny analysis, the novel strain formed a new branch within the genus Bacillus of the family Bacillaceae, and formed clusters with Bacillus thaohiensis NHI-38T (96.8%), Bacillus fengqiuensis NPK15T (96.7%), and Bacillus songklensis CAU 1033T (96.7%).
29492866	4	70	theme	CAU	693:695	arg1	%					708:708	96.7%	704:708	96.7%	704:708	Based on 16S rRNA gene phylogeny analysis, the novel strain formed a new branch within the genus Bacillus of the family Bacillaceae, and formed clusters with Bacillus thaohiensis NHI-38T (96.8%), Bacillus fengqiuensis NPK15T (96.7%), and Bacillus songklensis CAU 1033T (96.7%).
29492866	6	71	theme	DNA	893:895	arg1	content					870:876	The G + C content	860:876	content	870:876	The G + C content of the genomic DNA was 43.6 mol%.
29492866	6	71	theme	DNA	893:895	arg1	%					909:909	43.6 mol%	901:909	43.6 mol%	901:909	The G + C content of the genomic DNA was 43.6 mol%.
29492866	2	72	theme	motile	109:114	arg1	strain					162:167	A Gram-reaction-positive, strictly aerobic, motile, endospore- forming, and rod-shaped bacterial strain	65:167	A Gram-reaction-positive, strictly aerobic, motile, endospore- forming, and rod-shaped bacterial strain designated 135PIL107-10T	65:192	A Gram-reaction-positive, strictly aerobic, motile, endospore- forming, and rod-shaped bacterial strain designated 135PIL107-10T was isolated from a sponge on Jeju Island, and its taxonomic position was investigated using a polyphasic approach.
29492866	2	73	theme	Jeju	224:227	arg1	Island					229:234	Jeju Island	224:234	Jeju Island	224:234	A Gram-reaction-positive, strictly aerobic, motile, endospore- forming, and rod-shaped bacterial strain designated 135PIL107-10T was isolated from a sponge on Jeju Island, and its taxonomic position was investigated using a polyphasic approach.
29492866	4	74	theme	Bacillus	592:599	arg1	NHI-38T					613:619	Bacillus thaohiensis NHI-38T	592:619	Bacillus thaohiensis NHI-38T (96.8%)	592:627	Based on 16S rRNA gene phylogeny analysis, the novel strain formed a new branch within the genus Bacillus of the family Bacillaceae, and formed clusters with Bacillus thaohiensis NHI-38T (96.8%), Bacillus fengqiuensis NPK15T (96.7%), and Bacillus songklensis CAU 1033T (96.7%).
29492866	4	74	theme	Bacillus	592:599	arg1	%					626:626	96.8%	622:626	96.8%	622:626	Based on 16S rRNA gene phylogeny analysis, the novel strain formed a new branch within the genus Bacillus of the family Bacillaceae, and formed clusters with Bacillus thaohiensis NHI-38T (96.8%), Bacillus fengqiuensis NPK15T (96.7%), and Bacillus songklensis CAU 1033T (96.7%).
29492866	4	75	theme	gene	452:455	arg1	analysis					467:474	16S rRNA gene phylogeny analysis	443:474	16S rRNA gene phylogeny analysis	443:474	Based on 16S rRNA gene phylogeny analysis, the novel strain formed a new branch within the genus Bacillus of the family Bacillaceae, and formed clusters with Bacillus thaohiensis NHI-38T (96.8%), Bacillus fengqiuensis NPK15T (96.7%), and Bacillus songklensis CAU 1033T (96.7%).
29802973	6	0	theme	average	1488:1494	arg1	T2					1496:1497	average T2	1488:1497	average T2 of all knee regions (r = 0.23)	1488:1528	RESULTS Higher levels of sHA, sMMP3 and sCOMP were correlated (P < 0.05) with T2 of the lateral femur (r = 0.18 to 0.32) and lateral tibia (r = 0.17 to 0.23), and with average T2 of all knee regions (r = 0.23).
29802973	8	1	theme	morphologic	1602:1612	arg1	measures					1614:1621	the morphologic measures	1598:1621	the morphologic measures	1598:1621	Among the morphologic measures, sHA and sMMP3 was positively correlated (r = 0.17 to 0.21, P < 0.05) with meniscal damage.
29802973	5	2	theme	Type	1129:1132	arg1	uCtX-II					1147:1153	uCtX-II	1147:1153	uCtX-II	1147:1153	We focused on associations of serum hyaluronan (sHA), serum cartilage oligomeric matrix protein (sCOMP), serum matrix metalloproteinase-3 (sMMP3), and Urine Carboxy-Terminal Telepeptides of Type II Collagen (uCtX-II)) with MRI parameters (T2, WORMS), assessed using partial correlations adjusted for age, gender, body mass index (BMI), KL grade in both knees, and diabetes status.
29802973	5	2	theme	Type	1129:1132	arg1	Collagen					1137:1144	Type II Collagen	1129:1144	Type II Collagen (uCtX-II))	1129:1155	We focused on associations of serum hyaluronan (sHA), serum cartilage oligomeric matrix protein (sCOMP), serum matrix metalloproteinase-3 (sMMP3), and Urine Carboxy-Terminal Telepeptides of Type II Collagen (uCtX-II)) with MRI parameters (T2, WORMS), assessed using partial correlations adjusted for age, gender, body mass index (BMI), KL grade in both knees, and diabetes status.
29802973	2	3	theme	serum/urine	552:562	arg1	assays					574:579	available serum/urine biomarker assays	542:579	available serum/urine biomarker assays	542:579	DESIGN 141 subjects with Kellgren Lawrence (KL) grades 0-3 in the right knee and with available serum/urine biomarker assays were selected from the OAI.
29802973	5	4	with	hyaluronan	975:984	arg1	parameters					1166:1175	MRI parameters	1162:1175	MRI parameters (T2, WORMS)	1162:1187	We focused on associations of serum hyaluronan (sHA), serum cartilage oligomeric matrix protein (sCOMP), serum matrix metalloproteinase-3 (sMMP3), and Urine Carboxy-Terminal Telepeptides of Type II Collagen (uCtX-II)) with MRI parameters (T2, WORMS), assessed using partial correlations adjusted for age, gender, body mass index (BMI), KL grade in both knees, and diabetes status.
29802973	5	4	with	hyaluronan	975:984	arg1	T2					1178:1179	T2	1178:1179	T2	1178:1179	We focused on associations of serum hyaluronan (sHA), serum cartilage oligomeric matrix protein (sCOMP), serum matrix metalloproteinase-3 (sMMP3), and Urine Carboxy-Terminal Telepeptides of Type II Collagen (uCtX-II)) with MRI parameters (T2, WORMS), assessed using partial correlations adjusted for age, gender, body mass index (BMI), KL grade in both knees, and diabetes status.
29802973	5	5	theme	Collagen	1137:1144	arg1	sHA					987:989	sHA	987:989	sHA	987:989	We focused on associations of serum hyaluronan (sHA), serum cartilage oligomeric matrix protein (sCOMP), serum matrix metalloproteinase-3 (sMMP3), and Urine Carboxy-Terminal Telepeptides of Type II Collagen (uCtX-II)) with MRI parameters (T2, WORMS), assessed using partial correlations adjusted for age, gender, body mass index (BMI), KL grade in both knees, and diabetes status.
29802973	5	5	theme	Collagen	1137:1144	arg1	metalloproteinase-3					1057:1075	serum matrix metalloproteinase-3	1044:1075	serum matrix metalloproteinase-3 (sMMP3)	1044:1083	We focused on associations of serum hyaluronan (sHA), serum cartilage oligomeric matrix protein (sCOMP), serum matrix metalloproteinase-3 (sMMP3), and Urine Carboxy-Terminal Telepeptides of Type II Collagen (uCtX-II)) with MRI parameters (T2, WORMS), assessed using partial correlations adjusted for age, gender, body mass index (BMI), KL grade in both knees, and diabetes status.
29802973	5	5	theme	Collagen	1137:1144	arg1	protein					1027:1033	serum cartilage oligomeric matrix protein	993:1033	serum cartilage oligomeric matrix protein (sCOMP)	993:1041	We focused on associations of serum hyaluronan (sHA), serum cartilage oligomeric matrix protein (sCOMP), serum matrix metalloproteinase-3 (sMMP3), and Urine Carboxy-Terminal Telepeptides of Type II Collagen (uCtX-II)) with MRI parameters (T2, WORMS), assessed using partial correlations adjusted for age, gender, body mass index (BMI), KL grade in both knees, and diabetes status.
29802973	5	5	theme	Collagen	1137:1144	arg1	Telepeptides					1113:1124	Urine Carboxy-Terminal Telepeptides	1090:1124	Urine Carboxy-Terminal Telepeptides of Type II Collagen (uCtX-II)) with MRI parameters (T2, WORMS), assessed using partial correlations adjusted for age, gender, body mass index (BMI), KL grade in both knees, and diabetes status	1090:1317	We focused on associations of serum hyaluronan (sHA), serum cartilage oligomeric matrix protein (sCOMP), serum matrix metalloproteinase-3 (sMMP3), and Urine Carboxy-Terminal Telepeptides of Type II Collagen (uCtX-II)) with MRI parameters (T2, WORMS), assessed using partial correlations adjusted for age, gender, body mass index (BMI), KL grade in both knees, and diabetes status.
29802973	5	5	theme	Collagen	1137:1144	arg1	sCOMP					1036:1040	sCOMP	1036:1040	sCOMP	1036:1040	We focused on associations of serum hyaluronan (sHA), serum cartilage oligomeric matrix protein (sCOMP), serum matrix metalloproteinase-3 (sMMP3), and Urine Carboxy-Terminal Telepeptides of Type II Collagen (uCtX-II)) with MRI parameters (T2, WORMS), assessed using partial correlations adjusted for age, gender, body mass index (BMI), KL grade in both knees, and diabetes status.
29802973	5	5	theme	Collagen	1137:1144	arg1	sMMP3					1078:1082	sMMP3	1078:1082	sMMP3	1078:1082	We focused on associations of serum hyaluronan (sHA), serum cartilage oligomeric matrix protein (sCOMP), serum matrix metalloproteinase-3 (sMMP3), and Urine Carboxy-Terminal Telepeptides of Type II Collagen (uCtX-II)) with MRI parameters (T2, WORMS), assessed using partial correlations adjusted for age, gender, body mass index (BMI), KL grade in both knees, and diabetes status.
29802973	5	5	theme	Collagen	1137:1144	arg1	hyaluronan					975:984	serum hyaluronan	969:984	serum hyaluronan (sHA)	969:990	We focused on associations of serum hyaluronan (sHA), serum cartilage oligomeric matrix protein (sCOMP), serum matrix metalloproteinase-3 (sMMP3), and Urine Carboxy-Terminal Telepeptides of Type II Collagen (uCtX-II)) with MRI parameters (T2, WORMS), assessed using partial correlations adjusted for age, gender, body mass index (BMI), KL grade in both knees, and diabetes status.
29802973	4	6	theme	morphologic	800:810	arg1	resonance					842:850	Compartment-specific knee morphologic grading [whole-organ magnetic resonance	774:850	Compartment-specific knee morphologic grading [whole-organ magnetic resonance imaging score (WORMS)] in the cartilage, meniscus, and bone marrow	774:917	Compartment-specific knee morphologic grading [whole-organ magnetic resonance imaging score (WORMS)] in the cartilage, meniscus, and bone marrow was also performed.
29802973	9	7	theme	T2	1860:1861	arg1	measures					1863:1870	MRI T2 measures	1856:1870	MRI T2 measures of cartilage extra-cellular matrix degeneration	1856:1918	CONCLUSIONS This study suggests weak, but statistically significant, correlations between serum biomarkers of OA (sHA, sCOMP, and sMMP3) and MRI T2 measures of cartilage extra-cellular matrix degeneration.
29802973	4	8	theme	[whole-organ	820:831	arg1	resonance					842:850	Compartment-specific knee morphologic grading [whole-organ magnetic resonance	774:850	Compartment-specific knee morphologic grading [whole-organ magnetic resonance imaging score (WORMS)] in the cartilage, meniscus, and bone marrow	774:917	Compartment-specific knee morphologic grading [whole-organ magnetic resonance imaging score (WORMS)] in the cartilage, meniscus, and bone marrow was also performed.
29802973	3	9	theme	lateral	704:710	arg1	femur					712:716	lateral femur	704:716	lateral femur	704:716	Cartilage magnetic resonance imaging (MRI) T2 measurements were performed in the medial femur, lateral femur, medial tibia, lateral tibia, and patella compartments.
29802973	5	10	theme	KL	1275:1276	arg1	grade					1278:1282	KL grade	1275:1282	KL grade in both knees	1275:1296	We focused on associations of serum hyaluronan (sHA), serum cartilage oligomeric matrix protein (sCOMP), serum matrix metalloproteinase-3 (sMMP3), and Urine Carboxy-Terminal Telepeptides of Type II Collagen (uCtX-II)) with MRI parameters (T2, WORMS), assessed using partial correlations adjusted for age, gender, body mass index (BMI), KL grade in both knees, and diabetes status.
29802973	4	11	theme	magnetic	833:840	arg1	resonance					842:850	Compartment-specific knee morphologic grading [whole-organ magnetic resonance	774:850	Compartment-specific knee morphologic grading [whole-organ magnetic resonance imaging score (WORMS)] in the cartilage, meniscus, and bone marrow	774:917	Compartment-specific knee morphologic grading [whole-organ magnetic resonance imaging score (WORMS)] in the cartilage, meniscus, and bone marrow was also performed.
29802973	6	12	dep	correlated	1371:1380	arg1	P < 0.05					1383:1390	P < 0.05	1383:1390	P < 0.05	1383:1390	RESULTS Higher levels of sHA, sMMP3 and sCOMP were correlated (P < 0.05) with T2 of the lateral femur (r = 0.18 to 0.32) and lateral tibia (r = 0.17 to 0.23), and with average T2 of all knee regions (r = 0.23).
29802973	9	13	theme	extra-cellular	1885:1898	arg1	degeneration					1907:1918	cartilage extra-cellular matrix degeneration	1875:1918	cartilage extra-cellular matrix degeneration	1875:1918	CONCLUSIONS This study suggests weak, but statistically significant, correlations between serum biomarkers of OA (sHA, sCOMP, and sMMP3) and MRI T2 measures of cartilage extra-cellular matrix degeneration.
29802973	0	14	theme	Osteoarthritis	118:131	arg1	Initiative					133:142	the Osteoarthritis Initiative	114:142	the Osteoarthritis Initiative	114:142	Associations between molecular biomarkers and MR-based cartilage composition and knee joint morphology: data from the Osteoarthritis Initiative.
29802973	5	15	theme	serum	993:997	arg1	sCOMP					1036:1040	sCOMP	1036:1040	sCOMP	1036:1040	We focused on associations of serum hyaluronan (sHA), serum cartilage oligomeric matrix protein (sCOMP), serum matrix metalloproteinase-3 (sMMP3), and Urine Carboxy-Terminal Telepeptides of Type II Collagen (uCtX-II)) with MRI parameters (T2, WORMS), assessed using partial correlations adjusted for age, gender, body mass index (BMI), KL grade in both knees, and diabetes status.
29802973	5	15	theme	serum	993:997	arg1	protein					1027:1033	serum cartilage oligomeric matrix protein	993:1033	serum cartilage oligomeric matrix protein (sCOMP)	993:1041	We focused on associations of serum hyaluronan (sHA), serum cartilage oligomeric matrix protein (sCOMP), serum matrix metalloproteinase-3 (sMMP3), and Urine Carboxy-Terminal Telepeptides of Type II Collagen (uCtX-II)) with MRI parameters (T2, WORMS), assessed using partial correlations adjusted for age, gender, body mass index (BMI), KL grade in both knees, and diabetes status.
29802973	9	16	dep	CONCLUSIONS	1715:1725	arg1	suggests					1738:1745	suggests	1738:1745	suggests weak, but statistically significant, correlations between serum biomarkers of OA (sHA, sCOMP, and sMMP3) and MRI T2 measures of cartilage extra-cellular matrix degeneration	1738:1918	CONCLUSIONS This study suggests weak, but statistically significant, correlations between serum biomarkers of OA (sHA, sCOMP, and sMMP3) and MRI T2 measures of cartilage extra-cellular matrix degeneration.
29802973	9	17	theme	degeneration	1907:1918	arg1	biomarkers					1811:1820	serum biomarkers	1805:1820	serum biomarkers of OA (sHA, sCOMP, and sMMP3)	1805:1850	CONCLUSIONS This study suggests weak, but statistically significant, correlations between serum biomarkers of OA (sHA, sCOMP, and sMMP3) and MRI T2 measures of cartilage extra-cellular matrix degeneration.
29802973	9	17	theme	degeneration	1907:1918	arg1	measures					1863:1870	MRI T2 measures	1856:1870	MRI T2 measures of cartilage extra-cellular matrix degeneration	1856:1918	CONCLUSIONS This study suggests weak, but statistically significant, correlations between serum biomarkers of OA (sHA, sCOMP, and sMMP3) and MRI T2 measures of cartilage extra-cellular matrix degeneration.
29802973	5	18	theme	oligomeric	1009:1018	arg1	sCOMP					1036:1040	sCOMP	1036:1040	sCOMP	1036:1040	We focused on associations of serum hyaluronan (sHA), serum cartilage oligomeric matrix protein (sCOMP), serum matrix metalloproteinase-3 (sMMP3), and Urine Carboxy-Terminal Telepeptides of Type II Collagen (uCtX-II)) with MRI parameters (T2, WORMS), assessed using partial correlations adjusted for age, gender, body mass index (BMI), KL grade in both knees, and diabetes status.
29802973	5	18	theme	oligomeric	1009:1018	arg1	protein					1027:1033	serum cartilage oligomeric matrix protein	993:1033	serum cartilage oligomeric matrix protein (sCOMP)	993:1041	We focused on associations of serum hyaluronan (sHA), serum cartilage oligomeric matrix protein (sCOMP), serum matrix metalloproteinase-3 (sMMP3), and Urine Carboxy-Terminal Telepeptides of Type II Collagen (uCtX-II)) with MRI parameters (T2, WORMS), assessed using partial correlations adjusted for age, gender, body mass index (BMI), KL grade in both knees, and diabetes status.
29802973	6	19	theme	femur	1416:1420	arg1	T2					1398:1399	T2	1398:1399	T2 of the lateral femur (r = 0.18 to 0.32) and lateral tibia (r = 0.17 to 0.23)	1398:1476	RESULTS Higher levels of sHA, sMMP3 and sCOMP were correlated (P < 0.05) with T2 of the lateral femur (r = 0.18 to 0.32) and lateral tibia (r = 0.17 to 0.23), and with average T2 of all knee regions (r = 0.23).
29802973	1	20	theme	serum/urine	220:230	arg1	biomarkers					232:241	serum/urine biomarkers	220:241	serum/urine biomarkers for osteoarthritis and magnetic resonance (MR) imaging measures of cartilage composition and joint structure (cartilage, meniscus, and bone marrow)	220:389	OBJECTIVE The purpose of this study was to assess the associations between serum/urine biomarkers for osteoarthritis and magnetic resonance (MR) imaging measures of cartilage composition and joint structure (cartilage, meniscus, and bone marrow), using MR imaging data from the Osteoarthritis Initiative (OAI).
29802973	1	21	theme	MR	398:399	arg1	data					409:412	MR imaging data	398:412	MR imaging data from the Osteoarthritis Initiative (OAI)	398:453	OBJECTIVE The purpose of this study was to assess the associations between serum/urine biomarkers for osteoarthritis and magnetic resonance (MR) imaging measures of cartilage composition and joint structure (cartilage, meniscus, and bone marrow), using MR imaging data from the Osteoarthritis Initiative (OAI).
29802973	9	22	dep	OA	1825:1826	arg1	sMMP3					1845:1849	sMMP3	1845:1849	sMMP3	1845:1849	CONCLUSIONS This study suggests weak, but statistically significant, correlations between serum biomarkers of OA (sHA, sCOMP, and sMMP3) and MRI T2 measures of cartilage extra-cellular matrix degeneration.
29802973	9	22	dep	OA	1825:1826	arg1	sHA					1829:1831	sHA	1829:1831	sHA	1829:1831	CONCLUSIONS This study suggests weak, but statistically significant, correlations between serum biomarkers of OA (sHA, sCOMP, and sMMP3) and MRI T2 measures of cartilage extra-cellular matrix degeneration.
29802973	9	22	dep	OA	1825:1826	arg1	sCOMP					1834:1838	sCOMP	1834:1838	sCOMP	1834:1838	CONCLUSIONS This study suggests weak, but statistically significant, correlations between serum biomarkers of OA (sHA, sCOMP, and sMMP3) and MRI T2 measures of cartilage extra-cellular matrix degeneration.
29802973	2	23	theme	Kellgren	481:488	arg1	grades					504:509	Kellgren Lawrence (KL) grades 0-3	481:513	Kellgren Lawrence (KL) grades 0-3 in the right knee	481:531	DESIGN 141 subjects with Kellgren Lawrence (KL) grades 0-3 in the right knee and with available serum/urine biomarker assays were selected from the OAI.
29802973	2	23	theme	Kellgren	481:488	arg1	KL					500:501	KL	500:501	KL	500:501	DESIGN 141 subjects with Kellgren Lawrence (KL) grades 0-3 in the right knee and with available serum/urine biomarker assays were selected from the OAI.
29802973	3	24	theme	lateral	733:739	arg1	tibia					741:745	lateral tibia	733:745	lateral tibia	733:745	Cartilage magnetic resonance imaging (MRI) T2 measurements were performed in the medial femur, lateral femur, medial tibia, lateral tibia, and patella compartments.
29802973	3	25	dep	femur	697:701	arg1	compartments					760:771	compartments	760:771	compartments	760:771	Cartilage magnetic resonance imaging (MRI) T2 measurements were performed in the medial femur, lateral femur, medial tibia, lateral tibia, and patella compartments.
29802973	5	26	theme	MRI	1162:1164	arg1	parameters					1166:1175	MRI parameters	1162:1175	MRI parameters (T2, WORMS)	1162:1187	We focused on associations of serum hyaluronan (sHA), serum cartilage oligomeric matrix protein (sCOMP), serum matrix metalloproteinase-3 (sMMP3), and Urine Carboxy-Terminal Telepeptides of Type II Collagen (uCtX-II)) with MRI parameters (T2, WORMS), assessed using partial correlations adjusted for age, gender, body mass index (BMI), KL grade in both knees, and diabetes status.
29802973	5	26	theme	MRI	1162:1164	arg1	T2					1178:1179	T2	1178:1179	T2	1178:1179	We focused on associations of serum hyaluronan (sHA), serum cartilage oligomeric matrix protein (sCOMP), serum matrix metalloproteinase-3 (sMMP3), and Urine Carboxy-Terminal Telepeptides of Type II Collagen (uCtX-II)) with MRI parameters (T2, WORMS), assessed using partial correlations adjusted for age, gender, body mass index (BMI), KL grade in both knees, and diabetes status.
29802973	0	27	theme	joint	86:90	arg1	morphology					92:101	knee joint morphology	81:101	knee joint morphology	81:101	Associations between molecular biomarkers and MR-based cartilage composition and knee joint morphology: data from the Osteoarthritis Initiative.
29802973	5	28	theme	matrix	1050:1055	arg1	sMMP3					1078:1082	sMMP3	1078:1082	sMMP3	1078:1082	We focused on associations of serum hyaluronan (sHA), serum cartilage oligomeric matrix protein (sCOMP), serum matrix metalloproteinase-3 (sMMP3), and Urine Carboxy-Terminal Telepeptides of Type II Collagen (uCtX-II)) with MRI parameters (T2, WORMS), assessed using partial correlations adjusted for age, gender, body mass index (BMI), KL grade in both knees, and diabetes status.
29802973	5	28	theme	matrix	1050:1055	arg1	metalloproteinase-3					1057:1075	serum matrix metalloproteinase-3	1044:1075	serum matrix metalloproteinase-3 (sMMP3)	1044:1083	We focused on associations of serum hyaluronan (sHA), serum cartilage oligomeric matrix protein (sCOMP), serum matrix metalloproteinase-3 (sMMP3), and Urine Carboxy-Terminal Telepeptides of Type II Collagen (uCtX-II)) with MRI parameters (T2, WORMS), assessed using partial correlations adjusted for age, gender, body mass index (BMI), KL grade in both knees, and diabetes status.
29802973	2	29	with	subjects	467:474	arg1	grades					504:509	Kellgren Lawrence (KL) grades 0-3	481:513	Kellgren Lawrence (KL) grades 0-3 in the right knee	481:531	DESIGN 141 subjects with Kellgren Lawrence (KL) grades 0-3 in the right knee and with available serum/urine biomarker assays were selected from the OAI.
29802973	2	29	with	subjects	467:474	arg1	assays					574:579	available serum/urine biomarker assays	542:579	available serum/urine biomarker assays	542:579	DESIGN 141 subjects with Kellgren Lawrence (KL) grades 0-3 in the right knee and with available serum/urine biomarker assays were selected from the OAI.
29802973	2	29	with	subjects	467:474	arg1	KL					500:501	KL	500:501	KL	500:501	DESIGN 141 subjects with Kellgren Lawrence (KL) grades 0-3 in the right knee and with available serum/urine biomarker assays were selected from the OAI.
29802973	9	30	theme	serum	1805:1809	arg1	biomarkers					1811:1820	serum biomarkers	1805:1820	serum biomarkers of OA (sHA, sCOMP, and sMMP3)	1805:1850	CONCLUSIONS This study suggests weak, but statistically significant, correlations between serum biomarkers of OA (sHA, sCOMP, and sMMP3) and MRI T2 measures of cartilage extra-cellular matrix degeneration.
29802973	6	31	theme	knee	1506:1509	arg1	regions					1511:1517	all knee regions	1502:1517	all knee regions (r = 0.23)	1502:1528	RESULTS Higher levels of sHA, sMMP3 and sCOMP were correlated (P < 0.05) with T2 of the lateral femur (r = 0.18 to 0.32) and lateral tibia (r = 0.17 to 0.23), and with average T2 of all knee regions (r = 0.23).
29802973	6	31	theme	knee	1506:1509	arg1	r = 0.23					1520:1527	r = 0.23	1520:1527	r = 0.23	1520:1527	RESULTS Higher levels of sHA, sMMP3 and sCOMP were correlated (P < 0.05) with T2 of the lateral femur (r = 0.18 to 0.32) and lateral tibia (r = 0.17 to 0.23), and with average T2 of all knee regions (r = 0.23).
29802973	5	32	theme	protein	1027:1033	arg1	associations					953:964	associations	953:964	associations of serum hyaluronan (sHA), serum cartilage oligomeric matrix protein (sCOMP), serum matrix metalloproteinase-3 (sMMP3), and Urine Carboxy-Terminal Telepeptides of Type II Collagen (uCtX-II)) with MRI parameters (T2, WORMS), assessed using partial correlations adjusted for age, gender, body mass index (BMI), KL grade in both knees, and diabetes status	953:1317	We focused on associations of serum hyaluronan (sHA), serum cartilage oligomeric matrix protein (sCOMP), serum matrix metalloproteinase-3 (sMMP3), and Urine Carboxy-Terminal Telepeptides of Type II Collagen (uCtX-II)) with MRI parameters (T2, WORMS), assessed using partial correlations adjusted for age, gender, body mass index (BMI), KL grade in both knees, and diabetes status.
29802973	4	33	theme	bone	907:910	arg1	marrow					912:917	bone marrow	907:917	bone marrow	907:917	Compartment-specific knee morphologic grading [whole-organ magnetic resonance imaging score (WORMS)] in the cartilage, meniscus, and bone marrow was also performed.
29802973	6	34	dep	0.32	1435:1438	arg1	to					1432:1433	to	1432:1433	to	1432:1433	RESULTS Higher levels of sHA, sMMP3 and sCOMP were correlated (P < 0.05) with T2 of the lateral femur (r = 0.18 to 0.32) and lateral tibia (r = 0.17 to 0.23), and with average T2 of all knee regions (r = 0.23).
29802973	1	35	dep	composition	320:330	arg1	cartilage					353:361	cartilage	353:361	cartilage	353:361	OBJECTIVE The purpose of this study was to assess the associations between serum/urine biomarkers for osteoarthritis and magnetic resonance (MR) imaging measures of cartilage composition and joint structure (cartilage, meniscus, and bone marrow), using MR imaging data from the Osteoarthritis Initiative (OAI).
29802973	1	35	dep	composition	320:330	arg1	marrow					383:388	bone marrow	378:388	bone marrow	378:388	OBJECTIVE The purpose of this study was to assess the associations between serum/urine biomarkers for osteoarthritis and magnetic resonance (MR) imaging measures of cartilage composition and joint structure (cartilage, meniscus, and bone marrow), using MR imaging data from the Osteoarthritis Initiative (OAI).
29802973	1	35	dep	composition	320:330	arg1	meniscus					364:371	meniscus	364:371	meniscus	364:371	OBJECTIVE The purpose of this study was to assess the associations between serum/urine biomarkers for osteoarthritis and magnetic resonance (MR) imaging measures of cartilage composition and joint structure (cartilage, meniscus, and bone marrow), using MR imaging data from the Osteoarthritis Initiative (OAI).
29802973	8	36	theme	meniscal	1698:1705	arg1	damage					1707:1712	meniscal damage	1698:1712	meniscal damage	1698:1712	Among the morphologic measures, sHA and sMMP3 was positively correlated (r = 0.17 to 0.21, P < 0.05) with meniscal damage.
29802973	9	37	theme	MRI	1856:1858	arg1	measures					1863:1870	MRI T2 measures	1856:1870	MRI T2 measures of cartilage extra-cellular matrix degeneration	1856:1918	CONCLUSIONS This study suggests weak, but statistically significant, correlations between serum biomarkers of OA (sHA, sCOMP, and sMMP3) and MRI T2 measures of cartilage extra-cellular matrix degeneration.
29802973	5	38	from	gender	1244:1249	arg1	knees					1292:1296	both knees	1287:1296	both knees	1287:1296	We focused on associations of serum hyaluronan (sHA), serum cartilage oligomeric matrix protein (sCOMP), serum matrix metalloproteinase-3 (sMMP3), and Urine Carboxy-Terminal Telepeptides of Type II Collagen (uCtX-II)) with MRI parameters (T2, WORMS), assessed using partial correlations adjusted for age, gender, body mass index (BMI), KL grade in both knees, and diabetes status.
29802973	0	39	theme	molecular	21:29	arg1	biomarkers					31:40	molecular biomarkers	21:40	molecular biomarkers	21:40	Associations between molecular biomarkers and MR-based cartilage composition and knee joint morphology: data from the Osteoarthritis Initiative.
29802973	5	40	theme	Carboxy-Terminal	1096:1111	arg1	Telepeptides					1113:1124	Urine Carboxy-Terminal Telepeptides	1090:1124	Urine Carboxy-Terminal Telepeptides of Type II Collagen (uCtX-II)) with MRI parameters (T2, WORMS), assessed using partial correlations adjusted for age, gender, body mass index (BMI), KL grade in both knees, and diabetes status	1090:1317	We focused on associations of serum hyaluronan (sHA), serum cartilage oligomeric matrix protein (sCOMP), serum matrix metalloproteinase-3 (sMMP3), and Urine Carboxy-Terminal Telepeptides of Type II Collagen (uCtX-II)) with MRI parameters (T2, WORMS), assessed using partial correlations adjusted for age, gender, body mass index (BMI), KL grade in both knees, and diabetes status.
29802973	2	41	theme	DESIGN	456:461	arg1	subjects					467:474	DESIGN 141 subjects	456:474	DESIGN 141 subjects with Kellgren Lawrence (KL) grades 0-3 in the right knee and with available serum/urine biomarker assays	456:579	DESIGN 141 subjects with Kellgren Lawrence (KL) grades 0-3 in the right knee and with available serum/urine biomarker assays were selected from the OAI.
29802973	3	42	theme	magnetic	619:626	arg1	resonance					628:636	Cartilage magnetic resonance imaging	609:644	Cartilage magnetic resonance imaging (MRI) T2 measurements	609:666	Cartilage magnetic resonance imaging (MRI) T2 measurements were performed in the medial femur, lateral femur, medial tibia, lateral tibia, and patella compartments.
29802973	3	42	theme	magnetic	619:626	arg1	MRI					647:649	MRI	647:649	MRI	647:649	Cartilage magnetic resonance imaging (MRI) T2 measurements were performed in the medial femur, lateral femur, medial tibia, lateral tibia, and patella compartments.
29802973	5	43	theme	partial	1205:1211	arg1	correlations					1213:1224	partial correlations	1205:1224	partial correlations adjusted for age, gender, body mass index (BMI), KL grade in both knees, and diabetes status	1205:1317	We focused on associations of serum hyaluronan (sHA), serum cartilage oligomeric matrix protein (sCOMP), serum matrix metalloproteinase-3 (sMMP3), and Urine Carboxy-Terminal Telepeptides of Type II Collagen (uCtX-II)) with MRI parameters (T2, WORMS), assessed using partial correlations adjusted for age, gender, body mass index (BMI), KL grade in both knees, and diabetes status.
29802973	1	44	theme	cartilage	310:318	arg1	composition					320:330	cartilage composition	310:330	cartilage composition	310:330	OBJECTIVE The purpose of this study was to assess the associations between serum/urine biomarkers for osteoarthritis and magnetic resonance (MR) imaging measures of cartilage composition and joint structure (cartilage, meniscus, and bone marrow), using MR imaging data from the Osteoarthritis Initiative (OAI).
29802973	5	45	from	index	1262:1266	arg1	knees					1292:1296	both knees	1287:1296	both knees	1287:1296	We focused on associations of serum hyaluronan (sHA), serum cartilage oligomeric matrix protein (sCOMP), serum matrix metalloproteinase-3 (sMMP3), and Urine Carboxy-Terminal Telepeptides of Type II Collagen (uCtX-II)) with MRI parameters (T2, WORMS), assessed using partial correlations adjusted for age, gender, body mass index (BMI), KL grade in both knees, and diabetes status.
29802973	9	46	theme	OA	1825:1826	arg1	biomarkers					1811:1820	serum biomarkers	1805:1820	serum biomarkers of OA (sHA, sCOMP, and sMMP3)	1805:1850	CONCLUSIONS This study suggests weak, but statistically significant, correlations between serum biomarkers of OA (sHA, sCOMP, and sMMP3) and MRI T2 measures of cartilage extra-cellular matrix degeneration.
29802973	9	46	theme	OA	1825:1826	arg1	measures					1863:1870	MRI T2 measures	1856:1870	MRI T2 measures of cartilage extra-cellular matrix degeneration	1856:1918	CONCLUSIONS This study suggests weak, but statistically significant, correlations between serum biomarkers of OA (sHA, sCOMP, and sMMP3) and MRI T2 measures of cartilage extra-cellular matrix degeneration.
29802973	4	47	theme	Compartment-specific	774:793	arg1	resonance					842:850	Compartment-specific knee morphologic grading [whole-organ magnetic resonance	774:850	Compartment-specific knee morphologic grading [whole-organ magnetic resonance imaging score (WORMS)] in the cartilage, meniscus, and bone marrow	774:917	Compartment-specific knee morphologic grading [whole-organ magnetic resonance imaging score (WORMS)] in the cartilage, meniscus, and bone marrow was also performed.
29802973	0	48	theme	cartilage	55:63	arg1	composition					65:75	MR-based cartilage composition	46:75	MR-based cartilage composition	46:75	Associations between molecular biomarkers and MR-based cartilage composition and knee joint morphology: data from the Osteoarthritis Initiative.
29802973	1	49	theme	structure	342:350	arg1	measures					298:305	measures	298:305	measures of cartilage composition and joint structure (cartilage, meniscus, and bone marrow)	298:389	OBJECTIVE The purpose of this study was to assess the associations between serum/urine biomarkers for osteoarthritis and magnetic resonance (MR) imaging measures of cartilage composition and joint structure (cartilage, meniscus, and bone marrow), using MR imaging data from the Osteoarthritis Initiative (OAI).
29802973	5	50	theme	hyaluronan	975:984	arg1	associations					953:964	associations	953:964	associations of serum hyaluronan (sHA), serum cartilage oligomeric matrix protein (sCOMP), serum matrix metalloproteinase-3 (sMMP3), and Urine Carboxy-Terminal Telepeptides of Type II Collagen (uCtX-II)) with MRI parameters (T2, WORMS), assessed using partial correlations adjusted for age, gender, body mass index (BMI), KL grade in both knees, and diabetes status	953:1317	We focused on associations of serum hyaluronan (sHA), serum cartilage oligomeric matrix protein (sCOMP), serum matrix metalloproteinase-3 (sMMP3), and Urine Carboxy-Terminal Telepeptides of Type II Collagen (uCtX-II)) with MRI parameters (T2, WORMS), assessed using partial correlations adjusted for age, gender, body mass index (BMI), KL grade in both knees, and diabetes status.
29802973	5	51	from	grade	1278:1282	arg1	knees					1292:1296	both knees	1287:1296	both knees	1287:1296	We focused on associations of serum hyaluronan (sHA), serum cartilage oligomeric matrix protein (sCOMP), serum matrix metalloproteinase-3 (sMMP3), and Urine Carboxy-Terminal Telepeptides of Type II Collagen (uCtX-II)) with MRI parameters (T2, WORMS), assessed using partial correlations adjusted for age, gender, body mass index (BMI), KL grade in both knees, and diabetes status.
29802973	2	52	from	grades	504:509	arg1	knee					528:531	the right knee	518:531	the right knee	518:531	DESIGN 141 subjects with Kellgren Lawrence (KL) grades 0-3 in the right knee and with available serum/urine biomarker assays were selected from the OAI.
29802973	3	53	theme	imaging	638:644	arg1	resonance					628:636	Cartilage magnetic resonance imaging	609:644	Cartilage magnetic resonance imaging (MRI) T2 measurements	609:666	Cartilage magnetic resonance imaging (MRI) T2 measurements were performed in the medial femur, lateral femur, medial tibia, lateral tibia, and patella compartments.
29802973	3	53	theme	imaging	638:644	arg1	MRI					647:649	MRI	647:649	MRI	647:649	Cartilage magnetic resonance imaging (MRI) T2 measurements were performed in the medial femur, lateral femur, medial tibia, lateral tibia, and patella compartments.
29802973	0	54	dep	Associations	0:11	arg1	data					104:107	data	104:107	Associations between molecular biomarkers and MR-based cartilage composition and knee joint morphology: data from the Osteoarthritis Initiative.	0:143	Associations between molecular biomarkers and MR-based cartilage composition and knee joint morphology: data from the Osteoarthritis Initiative.
29802973	5	55	theme	body	1252:1255	arg1	index					1262:1266	body mass index	1252:1266	body mass index (BMI)	1252:1272	We focused on associations of serum hyaluronan (sHA), serum cartilage oligomeric matrix protein (sCOMP), serum matrix metalloproteinase-3 (sMMP3), and Urine Carboxy-Terminal Telepeptides of Type II Collagen (uCtX-II)) with MRI parameters (T2, WORMS), assessed using partial correlations adjusted for age, gender, body mass index (BMI), KL grade in both knees, and diabetes status.
29802973	5	55	theme	body	1252:1255	arg1	BMI					1269:1271	BMI	1269:1271	BMI	1269:1271	We focused on associations of serum hyaluronan (sHA), serum cartilage oligomeric matrix protein (sCOMP), serum matrix metalloproteinase-3 (sMMP3), and Urine Carboxy-Terminal Telepeptides of Type II Collagen (uCtX-II)) with MRI parameters (T2, WORMS), assessed using partial correlations adjusted for age, gender, body mass index (BMI), KL grade in both knees, and diabetes status.
29802973	1	56	theme	magnetic	266:273	arg1	resonance					275:283	magnetic resonance	266:283	magnetic resonance (MR) imaging measures of cartilage composition and joint structure (cartilage, meniscus, and bone marrow)	266:389	OBJECTIVE The purpose of this study was to assess the associations between serum/urine biomarkers for osteoarthritis and magnetic resonance (MR) imaging measures of cartilage composition and joint structure (cartilage, meniscus, and bone marrow), using MR imaging data from the Osteoarthritis Initiative (OAI).
29802973	1	56	theme	magnetic	266:273	arg1	MR					286:287	MR	286:287	MR	286:287	OBJECTIVE The purpose of this study was to assess the associations between serum/urine biomarkers for osteoarthritis and magnetic resonance (MR) imaging measures of cartilage composition and joint structure (cartilage, meniscus, and bone marrow), using MR imaging data from the Osteoarthritis Initiative (OAI).
29802973	2	57	theme	biomarker	564:572	arg1	assays					574:579	available serum/urine biomarker assays	542:579	available serum/urine biomarker assays	542:579	DESIGN 141 subjects with Kellgren Lawrence (KL) grades 0-3 in the right knee and with available serum/urine biomarker assays were selected from the OAI.
29802973	7	58	theme	patellar	1558:1565	arg1	T2					1567:1568	patellar T2	1558:1568	patellar T2 (r = 0.19, P = 0.04)	1558:1589	uCTXII was correlated with patellar T2 (r = 0.19, P = 0.04).
29802973	3	59	theme	T2	652:653	arg1	measurements					655:666	Cartilage magnetic resonance imaging (MRI) T2 measurements	609:666	Cartilage magnetic resonance imaging (MRI) T2 measurements	609:666	Cartilage magnetic resonance imaging (MRI) T2 measurements were performed in the medial femur, lateral femur, medial tibia, lateral tibia, and patella compartments.
29802973	1	60	theme	Osteoarthritis	423:436	arg1	Initiative					438:447	the Osteoarthritis Initiative	419:447	the Osteoarthritis Initiative (OAI)	419:453	OBJECTIVE The purpose of this study was to assess the associations between serum/urine biomarkers for osteoarthritis and magnetic resonance (MR) imaging measures of cartilage composition and joint structure (cartilage, meniscus, and bone marrow), using MR imaging data from the Osteoarthritis Initiative (OAI).
29802973	1	60	theme	Osteoarthritis	423:436	arg1	OAI					450:452	OAI	450:452	OAI	450:452	OBJECTIVE The purpose of this study was to assess the associations between serum/urine biomarkers for osteoarthritis and magnetic resonance (MR) imaging measures of cartilage composition and joint structure (cartilage, meniscus, and bone marrow), using MR imaging data from the Osteoarthritis Initiative (OAI).
29802973	2	61	theme	available	542:550	arg1	assays					574:579	available serum/urine biomarker assays	542:579	available serum/urine biomarker assays	542:579	DESIGN 141 subjects with Kellgren Lawrence (KL) grades 0-3 in the right knee and with available serum/urine biomarker assays were selected from the OAI.
29802973	5	62	dep	T2	1178:1179	arg1	WORMS					1182:1186	WORMS	1182:1186	WORMS	1182:1186	We focused on associations of serum hyaluronan (sHA), serum cartilage oligomeric matrix protein (sCOMP), serum matrix metalloproteinase-3 (sMMP3), and Urine Carboxy-Terminal Telepeptides of Type II Collagen (uCtX-II)) with MRI parameters (T2, WORMS), assessed using partial correlations adjusted for age, gender, body mass index (BMI), KL grade in both knees, and diabetes status.
29802973	3	63	theme	medial	690:695	arg1	femur					697:701	the medial femur	686:701	the medial femur	686:701	Cartilage magnetic resonance imaging (MRI) T2 measurements were performed in the medial femur, lateral femur, medial tibia, lateral tibia, and patella compartments.
29802973	5	64	with	metalloproteinase-3	1057:1075	arg1	parameters					1166:1175	MRI parameters	1162:1175	MRI parameters (T2, WORMS)	1162:1187	We focused on associations of serum hyaluronan (sHA), serum cartilage oligomeric matrix protein (sCOMP), serum matrix metalloproteinase-3 (sMMP3), and Urine Carboxy-Terminal Telepeptides of Type II Collagen (uCtX-II)) with MRI parameters (T2, WORMS), assessed using partial correlations adjusted for age, gender, body mass index (BMI), KL grade in both knees, and diabetes status.
29802973	5	64	with	metalloproteinase-3	1057:1075	arg1	T2					1178:1179	T2	1178:1179	T2	1178:1179	We focused on associations of serum hyaluronan (sHA), serum cartilage oligomeric matrix protein (sCOMP), serum matrix metalloproteinase-3 (sMMP3), and Urine Carboxy-Terminal Telepeptides of Type II Collagen (uCtX-II)) with MRI parameters (T2, WORMS), assessed using partial correlations adjusted for age, gender, body mass index (BMI), KL grade in both knees, and diabetes status.
29802973	1	65	theme	study	175:179	arg1	purpose					159:165	The purpose	155:165	The purpose of this study	155:179	OBJECTIVE The purpose of this study was to assess the associations between serum/urine biomarkers for osteoarthritis and magnetic resonance (MR) imaging measures of cartilage composition and joint structure (cartilage, meniscus, and bone marrow), using MR imaging data from the Osteoarthritis Initiative (OAI).
29802973	4	66	theme	knee	795:798	arg1	resonance					842:850	Compartment-specific knee morphologic grading [whole-organ magnetic resonance	774:850	Compartment-specific knee morphologic grading [whole-organ magnetic resonance imaging score (WORMS)] in the cartilage, meniscus, and bone marrow	774:917	Compartment-specific knee morphologic grading [whole-organ magnetic resonance imaging score (WORMS)] in the cartilage, meniscus, and bone marrow was also performed.
29802973	5	67	with	Telepeptides	1113:1124	arg1	parameters					1166:1175	MRI parameters	1162:1175	MRI parameters (T2, WORMS)	1162:1187	We focused on associations of serum hyaluronan (sHA), serum cartilage oligomeric matrix protein (sCOMP), serum matrix metalloproteinase-3 (sMMP3), and Urine Carboxy-Terminal Telepeptides of Type II Collagen (uCtX-II)) with MRI parameters (T2, WORMS), assessed using partial correlations adjusted for age, gender, body mass index (BMI), KL grade in both knees, and diabetes status.
29802973	5	67	with	Telepeptides	1113:1124	arg1	T2					1178:1179	T2	1178:1179	T2	1178:1179	We focused on associations of serum hyaluronan (sHA), serum cartilage oligomeric matrix protein (sCOMP), serum matrix metalloproteinase-3 (sMMP3), and Urine Carboxy-Terminal Telepeptides of Type II Collagen (uCtX-II)) with MRI parameters (T2, WORMS), assessed using partial correlations adjusted for age, gender, body mass index (BMI), KL grade in both knees, and diabetes status.
29802973	6	68	theme	sCOMP	1360:1364	arg1	levels					1335:1340	RESULTS Higher levels	1320:1340	RESULTS Higher levels of sHA, sMMP3 and sCOMP	1320:1364	RESULTS Higher levels of sHA, sMMP3 and sCOMP were correlated (P < 0.05) with T2 of the lateral femur (r = 0.18 to 0.32) and lateral tibia (r = 0.17 to 0.23), and with average T2 of all knee regions (r = 0.23).
29802973	5	69	from	status	1312:1317	arg1	knees					1292:1296	both knees	1287:1296	both knees	1287:1296	We focused on associations of serum hyaluronan (sHA), serum cartilage oligomeric matrix protein (sCOMP), serum matrix metalloproteinase-3 (sMMP3), and Urine Carboxy-Terminal Telepeptides of Type II Collagen (uCtX-II)) with MRI parameters (T2, WORMS), assessed using partial correlations adjusted for age, gender, body mass index (BMI), KL grade in both knees, and diabetes status.
29802973	4	70	theme	grading	812:818	arg1	resonance					842:850	Compartment-specific knee morphologic grading [whole-organ magnetic resonance	774:850	Compartment-specific knee morphologic grading [whole-organ magnetic resonance imaging score (WORMS)] in the cartilage, meniscus, and bone marrow	774:917	Compartment-specific knee morphologic grading [whole-organ magnetic resonance imaging score (WORMS)] in the cartilage, meniscus, and bone marrow was also performed.
29802973	9	71	theme	cartilage	1875:1883	arg1	degeneration					1907:1918	cartilage extra-cellular matrix degeneration	1875:1918	cartilage extra-cellular matrix degeneration	1875:1918	CONCLUSIONS This study suggests weak, but statistically significant, correlations between serum biomarkers of OA (sHA, sCOMP, and sMMP3) and MRI T2 measures of cartilage extra-cellular matrix degeneration.
29802973	6	72	theme	tibia	1453:1457	arg1	T2					1398:1399	T2	1398:1399	T2 of the lateral femur (r = 0.18 to 0.32) and lateral tibia (r = 0.17 to 0.23)	1398:1476	RESULTS Higher levels of sHA, sMMP3 and sCOMP were correlated (P < 0.05) with T2 of the lateral femur (r = 0.18 to 0.32) and lateral tibia (r = 0.17 to 0.23), and with average T2 of all knee regions (r = 0.23).
29802973	6	73	dep	0.23	1472:1475	arg1	to					1469:1470	to	1469:1470	to	1469:1470	RESULTS Higher levels of sHA, sMMP3 and sCOMP were correlated (P < 0.05) with T2 of the lateral femur (r = 0.18 to 0.32) and lateral tibia (r = 0.17 to 0.23), and with average T2 of all knee regions (r = 0.23).
29802973	9	74	theme	matrix	1900:1905	arg1	degeneration					1907:1918	cartilage extra-cellular matrix degeneration	1875:1918	cartilage extra-cellular matrix degeneration	1875:1918	CONCLUSIONS This study suggests weak, but statistically significant, correlations between serum biomarkers of OA (sHA, sCOMP, and sMMP3) and MRI T2 measures of cartilage extra-cellular matrix degeneration.
29802973	5	75	theme	cartilage	999:1007	arg1	sCOMP					1036:1040	sCOMP	1036:1040	sCOMP	1036:1040	We focused on associations of serum hyaluronan (sHA), serum cartilage oligomeric matrix protein (sCOMP), serum matrix metalloproteinase-3 (sMMP3), and Urine Carboxy-Terminal Telepeptides of Type II Collagen (uCtX-II)) with MRI parameters (T2, WORMS), assessed using partial correlations adjusted for age, gender, body mass index (BMI), KL grade in both knees, and diabetes status.
29802973	5	75	theme	cartilage	999:1007	arg1	protein					1027:1033	serum cartilage oligomeric matrix protein	993:1033	serum cartilage oligomeric matrix protein (sCOMP)	993:1041	We focused on associations of serum hyaluronan (sHA), serum cartilage oligomeric matrix protein (sCOMP), serum matrix metalloproteinase-3 (sMMP3), and Urine Carboxy-Terminal Telepeptides of Type II Collagen (uCtX-II)) with MRI parameters (T2, WORMS), assessed using partial correlations adjusted for age, gender, body mass index (BMI), KL grade in both knees, and diabetes status.
29802973	6	76	theme	sHA	1345:1347	arg1	levels					1335:1340	RESULTS Higher levels	1320:1340	RESULTS Higher levels of sHA, sMMP3 and sCOMP	1320:1364	RESULTS Higher levels of sHA, sMMP3 and sCOMP were correlated (P < 0.05) with T2 of the lateral femur (r = 0.18 to 0.32) and lateral tibia (r = 0.17 to 0.23), and with average T2 of all knee regions (r = 0.23).
29802973	5	77	theme	matrix	1020:1025	arg1	sCOMP					1036:1040	sCOMP	1036:1040	sCOMP	1036:1040	We focused on associations of serum hyaluronan (sHA), serum cartilage oligomeric matrix protein (sCOMP), serum matrix metalloproteinase-3 (sMMP3), and Urine Carboxy-Terminal Telepeptides of Type II Collagen (uCtX-II)) with MRI parameters (T2, WORMS), assessed using partial correlations adjusted for age, gender, body mass index (BMI), KL grade in both knees, and diabetes status.
29802973	5	77	theme	matrix	1020:1025	arg1	protein					1027:1033	serum cartilage oligomeric matrix protein	993:1033	serum cartilage oligomeric matrix protein (sCOMP)	993:1041	We focused on associations of serum hyaluronan (sHA), serum cartilage oligomeric matrix protein (sCOMP), serum matrix metalloproteinase-3 (sMMP3), and Urine Carboxy-Terminal Telepeptides of Type II Collagen (uCtX-II)) with MRI parameters (T2, WORMS), assessed using partial correlations adjusted for age, gender, body mass index (BMI), KL grade in both knees, and diabetes status.
29802973	6	78	theme	lateral	1408:1414	arg1	0.32					1435:1438	0.32	1435:1438	0.32	1435:1438	RESULTS Higher levels of sHA, sMMP3 and sCOMP were correlated (P < 0.05) with T2 of the lateral femur (r = 0.18 to 0.32) and lateral tibia (r = 0.17 to 0.23), and with average T2 of all knee regions (r = 0.23).
29802973	6	78	theme	lateral	1408:1414	arg1	femur					1416:1420	the lateral femur	1404:1420	the lateral femur (r = 0.18 to 0.32)	1404:1439	RESULTS Higher levels of sHA, sMMP3 and sCOMP were correlated (P < 0.05) with T2 of the lateral femur (r = 0.18 to 0.32) and lateral tibia (r = 0.17 to 0.23), and with average T2 of all knee regions (r = 0.23).
29802973	3	79	theme	medial	719:724	arg1	tibia					726:730	medial tibia	719:730	medial tibia	719:730	Cartilage magnetic resonance imaging (MRI) T2 measurements were performed in the medial femur, lateral femur, medial tibia, lateral tibia, and patella compartments.
29802973	1	80	theme	imaging	401:407	arg1	data					409:412	MR imaging data	398:412	MR imaging data from the Osteoarthritis Initiative (OAI)	398:453	OBJECTIVE The purpose of this study was to assess the associations between serum/urine biomarkers for osteoarthritis and magnetic resonance (MR) imaging measures of cartilage composition and joint structure (cartilage, meniscus, and bone marrow), using MR imaging data from the Osteoarthritis Initiative (OAI).
29802973	2	81	theme	right	522:526	arg1	knee					528:531	the right knee	518:531	the right knee	518:531	DESIGN 141 subjects with Kellgren Lawrence (KL) grades 0-3 in the right knee and with available serum/urine biomarker assays were selected from the OAI.
29802973	5	82	from	age	1239:1241	arg1	knees					1292:1296	both knees	1287:1296	both knees	1287:1296	We focused on associations of serum hyaluronan (sHA), serum cartilage oligomeric matrix protein (sCOMP), serum matrix metalloproteinase-3 (sMMP3), and Urine Carboxy-Terminal Telepeptides of Type II Collagen (uCtX-II)) with MRI parameters (T2, WORMS), assessed using partial correlations adjusted for age, gender, body mass index (BMI), KL grade in both knees, and diabetes status.
29802973	6	83	theme	sMMP3	1350:1354	arg1	levels					1335:1340	RESULTS Higher levels	1320:1340	RESULTS Higher levels of sHA, sMMP3 and sCOMP	1320:1364	RESULTS Higher levels of sHA, sMMP3 and sCOMP were correlated (P < 0.05) with T2 of the lateral femur (r = 0.18 to 0.32) and lateral tibia (r = 0.17 to 0.23), and with average T2 of all knee regions (r = 0.23).
29802973	5	84	with	protein	1027:1033	arg1	parameters					1166:1175	MRI parameters	1162:1175	MRI parameters (T2, WORMS)	1162:1187	We focused on associations of serum hyaluronan (sHA), serum cartilage oligomeric matrix protein (sCOMP), serum matrix metalloproteinase-3 (sMMP3), and Urine Carboxy-Terminal Telepeptides of Type II Collagen (uCtX-II)) with MRI parameters (T2, WORMS), assessed using partial correlations adjusted for age, gender, body mass index (BMI), KL grade in both knees, and diabetes status.
29802973	5	84	with	protein	1027:1033	arg1	T2					1178:1179	T2	1178:1179	T2	1178:1179	We focused on associations of serum hyaluronan (sHA), serum cartilage oligomeric matrix protein (sCOMP), serum matrix metalloproteinase-3 (sMMP3), and Urine Carboxy-Terminal Telepeptides of Type II Collagen (uCtX-II)) with MRI parameters (T2, WORMS), assessed using partial correlations adjusted for age, gender, body mass index (BMI), KL grade in both knees, and diabetes status.
29802973	0	85	theme	knee	81:84	arg1	morphology					92:101	knee joint morphology	81:101	knee joint morphology	81:101	Associations between molecular biomarkers and MR-based cartilage composition and knee joint morphology: data from the Osteoarthritis Initiative.
29802973	5	86	theme	serum	1044:1048	arg1	sMMP3					1078:1082	sMMP3	1078:1082	sMMP3	1078:1082	We focused on associations of serum hyaluronan (sHA), serum cartilage oligomeric matrix protein (sCOMP), serum matrix metalloproteinase-3 (sMMP3), and Urine Carboxy-Terminal Telepeptides of Type II Collagen (uCtX-II)) with MRI parameters (T2, WORMS), assessed using partial correlations adjusted for age, gender, body mass index (BMI), KL grade in both knees, and diabetes status.
29802973	5	86	theme	serum	1044:1048	arg1	metalloproteinase-3					1057:1075	serum matrix metalloproteinase-3	1044:1075	serum matrix metalloproteinase-3 (sMMP3)	1044:1083	We focused on associations of serum hyaluronan (sHA), serum cartilage oligomeric matrix protein (sCOMP), serum matrix metalloproteinase-3 (sMMP3), and Urine Carboxy-Terminal Telepeptides of Type II Collagen (uCtX-II)) with MRI parameters (T2, WORMS), assessed using partial correlations adjusted for age, gender, body mass index (BMI), KL grade in both knees, and diabetes status.
29802973	6	87	theme	regions	1511:1517	arg1	T2					1496:1497	average T2	1488:1497	average T2 of all knee regions (r = 0.23)	1488:1528	RESULTS Higher levels of sHA, sMMP3 and sCOMP were correlated (P < 0.05) with T2 of the lateral femur (r = 0.18 to 0.32) and lateral tibia (r = 0.17 to 0.23), and with average T2 of all knee regions (r = 0.23).
29802973	9	88	theme	weak	1747:1750	arg1	correlations					1784:1795	weak, but statistically significant, correlations	1747:1795	weak, but statistically significant, correlations between serum biomarkers of OA (sHA, sCOMP, and sMMP3) and MRI T2 measures of cartilage extra-cellular matrix degeneration	1747:1918	CONCLUSIONS This study suggests weak, but statistically significant, correlations between serum biomarkers of OA (sHA, sCOMP, and sMMP3) and MRI T2 measures of cartilage extra-cellular matrix degeneration.
29802973	5	89	theme	metalloproteinase-3	1057:1075	arg1	associations					953:964	associations	953:964	associations of serum hyaluronan (sHA), serum cartilage oligomeric matrix protein (sCOMP), serum matrix metalloproteinase-3 (sMMP3), and Urine Carboxy-Terminal Telepeptides of Type II Collagen (uCtX-II)) with MRI parameters (T2, WORMS), assessed using partial correlations adjusted for age, gender, body mass index (BMI), KL grade in both knees, and diabetes status	953:1317	We focused on associations of serum hyaluronan (sHA), serum cartilage oligomeric matrix protein (sCOMP), serum matrix metalloproteinase-3 (sMMP3), and Urine Carboxy-Terminal Telepeptides of Type II Collagen (uCtX-II)) with MRI parameters (T2, WORMS), assessed using partial correlations adjusted for age, gender, body mass index (BMI), KL grade in both knees, and diabetes status.
29802973	6	90	theme	lateral	1445:1451	arg1	0.23					1472:1475	0.23	1472:1475	0.23	1472:1475	RESULTS Higher levels of sHA, sMMP3 and sCOMP were correlated (P < 0.05) with T2 of the lateral femur (r = 0.18 to 0.32) and lateral tibia (r = 0.17 to 0.23), and with average T2 of all knee regions (r = 0.23).
29802973	6	90	theme	lateral	1445:1451	arg1	tibia					1453:1457	lateral tibia	1445:1457	lateral tibia (r = 0.17 to 0.23)	1445:1476	RESULTS Higher levels of sHA, sMMP3 and sCOMP were correlated (P < 0.05) with T2 of the lateral femur (r = 0.18 to 0.32) and lateral tibia (r = 0.17 to 0.23), and with average T2 of all knee regions (r = 0.23).
29802973	1	91	dep	OBJECTIVE	145:153	arg1	was					181:183	was	181:183	was	181:183	OBJECTIVE The purpose of this study was to assess the associations between serum/urine biomarkers for osteoarthritis and magnetic resonance (MR) imaging measures of cartilage composition and joint structure (cartilage, meniscus, and bone marrow), using MR imaging data from the Osteoarthritis Initiative (OAI).
29802973	2	92	theme	Lawrence	490:497	arg1	grades					504:509	Kellgren Lawrence (KL) grades 0-3	481:513	Kellgren Lawrence (KL) grades 0-3 in the right knee	481:531	DESIGN 141 subjects with Kellgren Lawrence (KL) grades 0-3 in the right knee and with available serum/urine biomarker assays were selected from the OAI.
29802973	2	92	theme	Lawrence	490:497	arg1	KL					500:501	KL	500:501	KL	500:501	DESIGN 141 subjects with Kellgren Lawrence (KL) grades 0-3 in the right knee and with available serum/urine biomarker assays were selected from the OAI.
29802973	5	93	theme	diabetes	1303:1310	arg1	status					1312:1317	diabetes status	1303:1317	diabetes status	1303:1317	We focused on associations of serum hyaluronan (sHA), serum cartilage oligomeric matrix protein (sCOMP), serum matrix metalloproteinase-3 (sMMP3), and Urine Carboxy-Terminal Telepeptides of Type II Collagen (uCtX-II)) with MRI parameters (T2, WORMS), assessed using partial correlations adjusted for age, gender, body mass index (BMI), KL grade in both knees, and diabetes status.
29802973	9	94	theme	significant	1771:1781	arg1	correlations					1784:1795	weak, but statistically significant, correlations	1747:1795	weak, but statistically significant, correlations between serum biomarkers of OA (sHA, sCOMP, and sMMP3) and MRI T2 measures of cartilage extra-cellular matrix degeneration	1747:1918	CONCLUSIONS This study suggests weak, but statistically significant, correlations between serum biomarkers of OA (sHA, sCOMP, and sMMP3) and MRI T2 measures of cartilage extra-cellular matrix degeneration.
29802973	1	95	theme	bone	378:381	arg1	marrow					383:388	bone marrow	378:388	bone marrow	378:388	OBJECTIVE The purpose of this study was to assess the associations between serum/urine biomarkers for osteoarthritis and magnetic resonance (MR) imaging measures of cartilage composition and joint structure (cartilage, meniscus, and bone marrow), using MR imaging data from the Osteoarthritis Initiative (OAI).
29802973	5	96	theme	Urine	1090:1094	arg1	Telepeptides					1113:1124	Urine Carboxy-Terminal Telepeptides	1090:1124	Urine Carboxy-Terminal Telepeptides of Type II Collagen (uCtX-II)) with MRI parameters (T2, WORMS), assessed using partial correlations adjusted for age, gender, body mass index (BMI), KL grade in both knees, and diabetes status	1090:1317	We focused on associations of serum hyaluronan (sHA), serum cartilage oligomeric matrix protein (sCOMP), serum matrix metalloproteinase-3 (sMMP3), and Urine Carboxy-Terminal Telepeptides of Type II Collagen (uCtX-II)) with MRI parameters (T2, WORMS), assessed using partial correlations adjusted for age, gender, body mass index (BMI), KL grade in both knees, and diabetes status.
29802973	8	97	dep	0.21	1677:1680	arg1	to					1674:1675	to	1674:1675	to	1674:1675	Among the morphologic measures, sHA and sMMP3 was positively correlated (r = 0.17 to 0.21, P < 0.05) with meniscal damage.
29802973	8	97	dep	0.21	1677:1680	arg1	P < 0.05					1683:1690	P < 0.05	1683:1690	P < 0.05	1683:1690	Among the morphologic measures, sHA and sMMP3 was positively correlated (r = 0.17 to 0.21, P < 0.05) with meniscal damage.
29802973	3	98	theme	Cartilage	609:617	arg1	resonance					628:636	Cartilage magnetic resonance imaging	609:644	Cartilage magnetic resonance imaging (MRI) T2 measurements	609:666	Cartilage magnetic resonance imaging (MRI) T2 measurements were performed in the medial femur, lateral femur, medial tibia, lateral tibia, and patella compartments.
29802973	3	98	theme	Cartilage	609:617	arg1	MRI					647:649	MRI	647:649	MRI	647:649	Cartilage magnetic resonance imaging (MRI) T2 measurements were performed in the medial femur, lateral femur, medial tibia, lateral tibia, and patella compartments.
29802973	0	99	theme	MR-based	46:53	arg1	composition					65:75	MR-based cartilage composition	46:75	MR-based cartilage composition	46:75	Associations between molecular biomarkers and MR-based cartilage composition and knee joint morphology: data from the Osteoarthritis Initiative.
29802973	5	100	theme	Telepeptides	1113:1124	arg1	associations					953:964	associations	953:964	associations of serum hyaluronan (sHA), serum cartilage oligomeric matrix protein (sCOMP), serum matrix metalloproteinase-3 (sMMP3), and Urine Carboxy-Terminal Telepeptides of Type II Collagen (uCtX-II)) with MRI parameters (T2, WORMS), assessed using partial correlations adjusted for age, gender, body mass index (BMI), KL grade in both knees, and diabetes status	953:1317	We focused on associations of serum hyaluronan (sHA), serum cartilage oligomeric matrix protein (sCOMP), serum matrix metalloproteinase-3 (sMMP3), and Urine Carboxy-Terminal Telepeptides of Type II Collagen (uCtX-II)) with MRI parameters (T2, WORMS), assessed using partial correlations adjusted for age, gender, body mass index (BMI), KL grade in both knees, and diabetes status.
29802973	5	101	theme	mass	1257:1260	arg1	index					1262:1266	body mass index	1252:1266	body mass index (BMI)	1252:1272	We focused on associations of serum hyaluronan (sHA), serum cartilage oligomeric matrix protein (sCOMP), serum matrix metalloproteinase-3 (sMMP3), and Urine Carboxy-Terminal Telepeptides of Type II Collagen (uCtX-II)) with MRI parameters (T2, WORMS), assessed using partial correlations adjusted for age, gender, body mass index (BMI), KL grade in both knees, and diabetes status.
29802973	5	101	theme	mass	1257:1260	arg1	BMI					1269:1271	BMI	1269:1271	BMI	1269:1271	We focused on associations of serum hyaluronan (sHA), serum cartilage oligomeric matrix protein (sCOMP), serum matrix metalloproteinase-3 (sMMP3), and Urine Carboxy-Terminal Telepeptides of Type II Collagen (uCtX-II)) with MRI parameters (T2, WORMS), assessed using partial correlations adjusted for age, gender, body mass index (BMI), KL grade in both knees, and diabetes status.
29802973	1	102	theme	composition	320:330	arg1	measures					298:305	measures	298:305	measures of cartilage composition and joint structure (cartilage, meniscus, and bone marrow)	298:389	OBJECTIVE The purpose of this study was to assess the associations between serum/urine biomarkers for osteoarthritis and magnetic resonance (MR) imaging measures of cartilage composition and joint structure (cartilage, meniscus, and bone marrow), using MR imaging data from the Osteoarthritis Initiative (OAI).
29802973	5	103	theme	serum	969:973	arg1	sHA					987:989	sHA	987:989	sHA	987:989	We focused on associations of serum hyaluronan (sHA), serum cartilage oligomeric matrix protein (sCOMP), serum matrix metalloproteinase-3 (sMMP3), and Urine Carboxy-Terminal Telepeptides of Type II Collagen (uCtX-II)) with MRI parameters (T2, WORMS), assessed using partial correlations adjusted for age, gender, body mass index (BMI), KL grade in both knees, and diabetes status.
29802973	5	103	theme	serum	969:973	arg1	hyaluronan					975:984	serum hyaluronan	969:984	serum hyaluronan (sHA)	969:990	We focused on associations of serum hyaluronan (sHA), serum cartilage oligomeric matrix protein (sCOMP), serum matrix metalloproteinase-3 (sMMP3), and Urine Carboxy-Terminal Telepeptides of Type II Collagen (uCtX-II)) with MRI parameters (T2, WORMS), assessed using partial correlations adjusted for age, gender, body mass index (BMI), KL grade in both knees, and diabetes status.
29802973	8	104	dep	correlated	1653:1662	arg1	0.21					1677:1680	0.21	1677:1680	0.21	1677:1680	Among the morphologic measures, sHA and sMMP3 was positively correlated (r = 0.17 to 0.21, P < 0.05) with meniscal damage.
29802973	1	105	theme	joint	336:340	arg1	structure					342:350	joint structure	336:350	joint structure	336:350	OBJECTIVE The purpose of this study was to assess the associations between serum/urine biomarkers for osteoarthritis and magnetic resonance (MR) imaging measures of cartilage composition and joint structure (cartilage, meniscus, and bone marrow), using MR imaging data from the Osteoarthritis Initiative (OAI).
29802973	3	106	theme	resonance	628:636	arg1	measurements					655:666	Cartilage magnetic resonance imaging (MRI) T2 measurements	609:666	Cartilage magnetic resonance imaging (MRI) T2 measurements	609:666	Cartilage magnetic resonance imaging (MRI) T2 measurements were performed in the medial femur, lateral femur, medial tibia, lateral tibia, and patella compartments.
29802973	6	107	theme	Higher	1328:1333	arg1	levels					1335:1340	RESULTS Higher levels	1320:1340	RESULTS Higher levels of sHA, sMMP3 and sCOMP	1320:1364	RESULTS Higher levels of sHA, sMMP3 and sCOMP were correlated (P < 0.05) with T2 of the lateral femur (r = 0.18 to 0.32) and lateral tibia (r = 0.17 to 0.23), and with average T2 of all knee regions (r = 0.23).
29802973	1	108	from	Initiative	438:447	arg1	data					409:412	MR imaging data	398:412	MR imaging data from the Osteoarthritis Initiative (OAI)	398:453	OBJECTIVE The purpose of this study was to assess the associations between serum/urine biomarkers for osteoarthritis and magnetic resonance (MR) imaging measures of cartilage composition and joint structure (cartilage, meniscus, and bone marrow), using MR imaging data from the Osteoarthritis Initiative (OAI).
29802973	7	109	dep	T2	1567:1568	arg1	P = 0.04					1581:1588	P = 0.04	1581:1588	P = 0.04	1581:1588	uCTXII was correlated with patellar T2 (r = 0.19, P = 0.04).
29802973	7	109	dep	T2	1567:1568	arg1	r = 0.19					1571:1578	r = 0.19	1571:1578	r = 0.19	1571:1578	uCTXII was correlated with patellar T2 (r = 0.19, P = 0.04).
29802973	0	110	from	Initiative	133:142	arg1	data					104:107	data	104:107	Associations between molecular biomarkers and MR-based cartilage composition and knee joint morphology: data from the Osteoarthritis Initiative.	0:143	Associations between molecular biomarkers and MR-based cartilage composition and knee joint morphology: data from the Osteoarthritis Initiative.
31918849	3	0	theme	additional	1065:1074	arg1	benefits					1076:1083	additional benefits	1065:1083	additional benefits regarding analyte zone focusing	1065:1115	A special focus was given to optimize the composition of the sample matrix to maintain the solubility of the analytes within the sample zone while gaining additional benefits regarding analyte zone focusing.
31918849	8	1	theme	patients	1974:1981	arg1	regimen					1963:1969	the treatment regimen	1949:1969	the treatment regimen of patients with hepatitis C virus infection	1949:2014	The selectivity of the developed method for determination of the studied compounds in their pharmaceutical dosage forms or in the presence of ribavirin (RIB) or elbasvir (ELB), which are other prescribed medications in the treatment regimen of patients with hepatitis C virus infection, is demonstrated.
31918849	2	2	theme	background	750:759	arg1	electrolyte					761:771	a basic background electrolyte	742:771	a basic background electrolyte (BGE) composed of 25 mmol L-1 SDS + 20% (v/v)	742:817	For the first time, a successful separation of all the investigated compounds was achieved in less than 8 min using a basic background electrolyte (BGE) composed of 25 mmol L-1 SDS + 20% (v/v) ACN (acetonitrile) in 10 mmol L-1 disodium tetraborate buffer (final apparent pH is 9.90).
31918849	2	2	theme	background	750:759	arg1	BGE					774:776	BGE	774:776	BGE	774:776	For the first time, a successful separation of all the investigated compounds was achieved in less than 8 min using a basic background electrolyte (BGE) composed of 25 mmol L-1 SDS + 20% (v/v) ACN (acetonitrile) in 10 mmol L-1 disodium tetraborate buffer (final apparent pH is 9.90).
31918849	8	3	theme	hepatitis	1988:1996	arg1	virus					2000:2004	hepatitis C virus	1988:2004	hepatitis C virus infection	1988:2014	The selectivity of the developed method for determination of the studied compounds in their pharmaceutical dosage forms or in the presence of ribavirin (RIB) or elbasvir (ELB), which are other prescribed medications in the treatment regimen of patients with hepatitis C virus infection, is demonstrated.
31918849	2	4	theme	compounds	694:702	arg1	separation					659:668	a successful separation	646:668	a successful separation of all the investigated compounds	646:702	For the first time, a successful separation of all the investigated compounds was achieved in less than 8 min using a basic background electrolyte (BGE) composed of 25 mmol L-1 SDS + 20% (v/v) ACN (acetonitrile) in 10 mmol L-1 disodium tetraborate buffer (final apparent pH is 9.90).
31918849	0	5	with	combination	118:128	arg1	drugs					175:179	its co-formulated hepatitis C antiviral drugs	135:179	its co-formulated hepatitis C antiviral drugs	135:179	Optimization of a sensitive and robust strategy for micellar electrokinetic chromatographic analysis of sofosbuvir in combination with its co-formulated hepatitis C antiviral drugs.
31918849	8	6	theme	virus	2000:2004	arg1	infection					2006:2014	hepatitis C virus infection	1988:2014	hepatitis C virus infection	1988:2014	The selectivity of the developed method for determination of the studied compounds in their pharmaceutical dosage forms or in the presence of ribavirin (RIB) or elbasvir (ELB), which are other prescribed medications in the treatment regimen of patients with hepatitis C virus infection, is demonstrated.
31918849	8	7	theme	method	1763:1768	arg1	selectivity					1734:1744	The selectivity	1730:1744	The selectivity	1730:1744	The selectivity of the developed method for determination of the studied compounds in their pharmaceutical dosage forms or in the presence of ribavirin (RIB) or elbasvir (ELB), which are other prescribed medications in the treatment regimen of patients with hepatitis C virus infection, is demonstrated.
31918849	8	7	theme	method	1763:1768	arg1	medications					1934:1944	other prescribed medications	1917:1944	other prescribed medications in the treatment regimen of patients with hepatitis C virus infection	1917:2014	The selectivity of the developed method for determination of the studied compounds in their pharmaceutical dosage forms or in the presence of ribavirin (RIB) or elbasvir (ELB), which are other prescribed medications in the treatment regimen of patients with hepatitis C virus infection, is demonstrated.
31918849	4	8	theme	substantial	1258:1268	arg1	impact					1279:1284	a substantial positive impact	1256:1284	a substantial positive impact on the obtained enrichment efficiency	1256:1322	It was found that replacing phosphoric acid (as a sample matrix) with a zwitterionic/isoelectric buffering compound (L-glutamic acid) has a substantial positive impact on the obtained enrichment efficiency.
31918849	1	9	theme	previous	195:202	arg1	work					204:207	our previous work	191:207	our previous work with "pseudostationary-ion exchanger sweeping"	191:254	Based on our previous work with "pseudostationary-ion exchanger sweeping", we use this strategy to develop a sensitive, reliable and robust method for the analysis of the newly-FDA approved hepatitis C antiviral drugs namely; sofosbuvir (SOV), daclatasvir (DAC), ledipasvir (LED) and velpatasvir (VEP) in their pure forms and co-formulated pharmaceutical dosage forms using micellar electrokinetic chromatography (MEKC) as a separation method.
31918849	3	10	theme	special	912:918	arg1	focus					920:924	A special focus	910:924	A special focus	910:924	A special focus was given to optimize the composition of the sample matrix to maintain the solubility of the analytes within the sample zone while gaining additional benefits regarding analyte zone focusing.
31918849	2	11	theme	less	720:723	arg1	ACN					819:821	less than 8 min using a basic background electrolyte (BGE) composed of 25 mmol L-1 SDS + 20% (v/v) ACN	720:821	less than 8 min using a basic background electrolyte (BGE) composed of 25 mmol L-1 SDS + 20% (v/v) ACN (acetonitrile)	720:836	For the first time, a successful separation of all the investigated compounds was achieved in less than 8 min using a basic background electrolyte (BGE) composed of 25 mmol L-1 SDS + 20% (v/v) ACN (acetonitrile) in 10 mmol L-1 disodium tetraborate buffer (final apparent pH is 9.90).
31918849	2	11	theme	less	720:723	arg1	acetonitrile					824:835	acetonitrile	824:835	acetonitrile	824:835	For the first time, a successful separation of all the investigated compounds was achieved in less than 8 min using a basic background electrolyte (BGE) composed of 25 mmol L-1 SDS + 20% (v/v) ACN (acetonitrile) in 10 mmol L-1 disodium tetraborate buffer (final apparent pH is 9.90).
31918849	1	12	theme	separation	607:616	arg1	chromatography					580:593	micellar electrokinetic chromatography	556:593	micellar electrokinetic chromatography (MEKC)	556:600	Based on our previous work with "pseudostationary-ion exchanger sweeping", we use this strategy to develop a sensitive, reliable and robust method for the analysis of the newly-FDA approved hepatitis C antiviral drugs namely; sofosbuvir (SOV), daclatasvir (DAC), ledipasvir (LED) and velpatasvir (VEP) in their pure forms and co-formulated pharmaceutical dosage forms using micellar electrokinetic chromatography (MEKC) as a separation method.
31918849	1	12	theme	separation	607:616	arg1	method					618:623	a separation method	605:623	a separation method	605:623	Based on our previous work with "pseudostationary-ion exchanger sweeping", we use this strategy to develop a sensitive, reliable and robust method for the analysis of the newly-FDA approved hepatitis C antiviral drugs namely; sofosbuvir (SOV), daclatasvir (DAC), ledipasvir (LED) and velpatasvir (VEP) in their pure forms and co-formulated pharmaceutical dosage forms using micellar electrokinetic chromatography (MEKC) as a separation method.
31918849	2	13	dep	pH	897:898	arg1	9.90					903:906	9.90	903:906	9.90	903:906	For the first time, a successful separation of all the investigated compounds was achieved in less than 8 min using a basic background electrolyte (BGE) composed of 25 mmol L-1 SDS + 20% (v/v) ACN (acetonitrile) in 10 mmol L-1 disodium tetraborate buffer (final apparent pH is 9.90).
31918849	1	14	theme	hepatitis	372:380	arg1	drugs					394:398	the newly-FDA approved hepatitis C antiviral drugs	349:398	the newly-FDA approved hepatitis C antiviral drugs namely	349:405	Based on our previous work with "pseudostationary-ion exchanger sweeping", we use this strategy to develop a sensitive, reliable and robust method for the analysis of the newly-FDA approved hepatitis C antiviral drugs namely; sofosbuvir (SOV), daclatasvir (DAC), ledipasvir (LED) and velpatasvir (VEP) in their pure forms and co-formulated pharmaceutical dosage forms using micellar electrokinetic chromatography (MEKC) as a separation method.
31918849	8	15	theme	elbasvir	1891:1898	arg1	presence					1860:1867	the presence	1856:1867	the presence of ribavirin (RIB) or elbasvir (ELB)	1856:1904	The selectivity of the developed method for determination of the studied compounds in their pharmaceutical dosage forms or in the presence of ribavirin (RIB) or elbasvir (ELB), which are other prescribed medications in the treatment regimen of patients with hepatitis C virus infection, is demonstrated.
31918849	0	16	theme	co-formulated	139:151	arg1	drugs					175:179	its co-formulated hepatitis C antiviral drugs	135:179	its co-formulated hepatitis C antiviral drugs	135:179	Optimization of a sensitive and robust strategy for micellar electrokinetic chromatographic analysis of sofosbuvir in combination with its co-formulated hepatitis C antiviral drugs.
31918849	1	17	theme	antiviral	384:392	arg1	drugs					394:398	the newly-FDA approved hepatitis C antiviral drugs	349:398	the newly-FDA approved hepatitis C antiviral drugs namely	349:405	Based on our previous work with "pseudostationary-ion exchanger sweeping", we use this strategy to develop a sensitive, reliable and robust method for the analysis of the newly-FDA approved hepatitis C antiviral drugs namely; sofosbuvir (SOV), daclatasvir (DAC), ledipasvir (LED) and velpatasvir (VEP) in their pure forms and co-formulated pharmaceutical dosage forms using micellar electrokinetic chromatography (MEKC) as a separation method.
31918849	1	18	theme	pseudostationary-ion	215:234	arg1	"					254:254	"pseudostationary-ion exchanger sweeping"	214:254	"pseudostationary-ion exchanger sweeping"	214:254	Based on our previous work with "pseudostationary-ion exchanger sweeping", we use this strategy to develop a sensitive, reliable and robust method for the analysis of the newly-FDA approved hepatitis C antiviral drugs namely; sofosbuvir (SOV), daclatasvir (DAC), ledipasvir (LED) and velpatasvir (VEP) in their pure forms and co-formulated pharmaceutical dosage forms using micellar electrokinetic chromatography (MEKC) as a separation method.
31918849	2	19	dep	buffer	874:879	arg1	pH					897:898	final apparent pH	882:898	final apparent pH is 9.90	882:906	For the first time, a successful separation of all the investigated compounds was achieved in less than 8 min using a basic background electrolyte (BGE) composed of 25 mmol L-1 SDS + 20% (v/v) ACN (acetonitrile) in 10 mmol L-1 disodium tetraborate buffer (final apparent pH is 9.90).
31918849	0	20	theme	C	163:163	arg1	drugs					175:179	its co-formulated hepatitis C antiviral drugs	135:179	its co-formulated hepatitis C antiviral drugs	135:179	Optimization of a sensitive and robust strategy for micellar electrokinetic chromatographic analysis of sofosbuvir in combination with its co-formulated hepatitis C antiviral drugs.
31918849	2	21	theme	first	634:638	arg1	time					640:643	the first time	630:643	the first time	630:643	For the first time, a successful separation of all the investigated compounds was achieved in less than 8 min using a basic background electrolyte (BGE) composed of 25 mmol L-1 SDS + 20% (v/v) ACN (acetonitrile) in 10 mmol L-1 disodium tetraborate buffer (final apparent pH is 9.90).
31918849	6	22	theme	full	1443:1446	arg1	study					1459:1463	A full validation study	1441:1463	A full validation study	1441:1463	A full validation study is performed based on the pharmacopeial and ICH guidelines.
31918849	1	23	theme	micellar	556:563	arg1	chromatography					580:593	micellar electrokinetic chromatography	556:593	micellar electrokinetic chromatography (MEKC)	556:600	Based on our previous work with "pseudostationary-ion exchanger sweeping", we use this strategy to develop a sensitive, reliable and robust method for the analysis of the newly-FDA approved hepatitis C antiviral drugs namely; sofosbuvir (SOV), daclatasvir (DAC), ledipasvir (LED) and velpatasvir (VEP) in their pure forms and co-formulated pharmaceutical dosage forms using micellar electrokinetic chromatography (MEKC) as a separation method.
31918849	1	23	theme	micellar	556:563	arg1	method					618:623	a separation method	605:623	a separation method	605:623	Based on our previous work with "pseudostationary-ion exchanger sweeping", we use this strategy to develop a sensitive, reliable and robust method for the analysis of the newly-FDA approved hepatitis C antiviral drugs namely; sofosbuvir (SOV), daclatasvir (DAC), ledipasvir (LED) and velpatasvir (VEP) in their pure forms and co-formulated pharmaceutical dosage forms using micellar electrokinetic chromatography (MEKC) as a separation method.
31918849	1	23	theme	micellar	556:563	arg1	MEKC					596:599	MEKC	596:599	MEKC	596:599	Based on our previous work with "pseudostationary-ion exchanger sweeping", we use this strategy to develop a sensitive, reliable and robust method for the analysis of the newly-FDA approved hepatitis C antiviral drugs namely; sofosbuvir (SOV), daclatasvir (DAC), ledipasvir (LED) and velpatasvir (VEP) in their pure forms and co-formulated pharmaceutical dosage forms using micellar electrokinetic chromatography (MEKC) as a separation method.
31918849	0	24	theme	micellar	52:59	arg1	analysis					92:99	micellar electrokinetic chromatographic analysis	52:99	micellar electrokinetic chromatographic analysis of sofosbuvir	52:113	Optimization of a sensitive and robust strategy for micellar electrokinetic chromatographic analysis of sofosbuvir in combination with its co-formulated hepatitis C antiviral drugs.
31918849	2	25	theme	tetraborate	862:872	arg1	buffer					874:879	10 mmol L-1 disodium tetraborate buffer	841:879	10 mmol L-1 disodium tetraborate buffer (final apparent pH is 9.90)	841:907	For the first time, a successful separation of all the investigated compounds was achieved in less than 8 min using a basic background electrolyte (BGE) composed of 25 mmol L-1 SDS + 20% (v/v) ACN (acetonitrile) in 10 mmol L-1 disodium tetraborate buffer (final apparent pH is 9.90).
31918849	2	26	theme	successful	648:657	arg1	separation					659:668	a successful separation	646:668	a successful separation of all the investigated compounds	646:702	For the first time, a successful separation of all the investigated compounds was achieved in less than 8 min using a basic background electrolyte (BGE) composed of 25 mmol L-1 SDS + 20% (v/v) ACN (acetonitrile) in 10 mmol L-1 disodium tetraborate buffer (final apparent pH is 9.90).
31918849	0	27	theme	chromatographic	76:90	arg1	analysis					92:99	micellar electrokinetic chromatographic analysis	52:99	micellar electrokinetic chromatographic analysis of sofosbuvir	52:113	Optimization of a sensitive and robust strategy for micellar electrokinetic chromatographic analysis of sofosbuvir in combination with its co-formulated hepatitis C antiviral drugs.
31918849	9	28	theme	migration	2255:2263	arg1	time					2265:2268	migration time	2255:2268	migration time	2255:2268	It is shown that with acidic sample matrix and basic BGE, an efficient and precise approach was designed in which analyte adsorption on the capillary wall was minimized while keeping repeatable peak height, peak area and migration time together with the highest possible enrichment efficiency.
31918849	9	28	theme	migration	2255:2263	arg1	peak					2228:2231	repeatable peak height	2217:2238	repeatable peak height	2217:2238	It is shown that with acidic sample matrix and basic BGE, an efficient and precise approach was designed in which analyte adsorption on the capillary wall was minimized while keeping repeatable peak height, peak area and migration time together with the highest possible enrichment efficiency.
31918849	0	29	from	Optimization	0:11	arg1	combination					118:128	combination	118:128	combination with its co-formulated hepatitis C antiviral drugs	118:179	Optimization of a sensitive and robust strategy for micellar electrokinetic chromatographic analysis of sofosbuvir in combination with its co-formulated hepatitis C antiviral drugs.
31918849	4	30	theme	enrichment	1302:1311	arg1	efficiency					1313:1322	the obtained enrichment efficiency	1289:1322	the obtained enrichment efficiency	1289:1322	It was found that replacing phosphoric acid (as a sample matrix) with a zwitterionic/isoelectric buffering compound (L-glutamic acid) has a substantial positive impact on the obtained enrichment efficiency.
31918849	6	31	theme	ICH	1509:1511	arg1	guidelines					1513:1522	the pharmacopeial and ICH guidelines	1487:1522	guidelines	1513:1522	A full validation study is performed based on the pharmacopeial and ICH guidelines.
31918849	7	32	theme	quantitation	1562:1573	arg1	limits					1538:1543	The obtained limits	1525:1543	The obtained limits of detection and quantitation	1525:1573	The obtained limits of detection and quantitation are as low as 0.63 and 1.3 μg mL-1; respectively for SOV and DAC and 1.3 and 2.5 μg mL-1; respectively for LED and VEP using UV-DAD as a detection method.
31918849	7	32	theme	quantitation	1562:1573	arg1	low					1582:1584	low	1582:1584	low	1582:1584	The obtained limits of detection and quantitation are as low as 0.63 and 1.3 μg mL-1; respectively for SOV and DAC and 1.3 and 2.5 μg mL-1; respectively for LED and VEP using UV-DAD as a detection method.
31918849	2	33	theme	apparent	888:895	arg1	pH					897:898	final apparent pH	882:898	final apparent pH is 9.90	882:906	For the first time, a successful separation of all the investigated compounds was achieved in less than 8 min using a basic background electrolyte (BGE) composed of 25 mmol L-1 SDS + 20% (v/v) ACN (acetonitrile) in 10 mmol L-1 disodium tetraborate buffer (final apparent pH is 9.90).
31918849	9	34	theme	acidic	2056:2061	arg1	matrix					2070:2075	acidic sample matrix	2056:2075	acidic sample matrix	2056:2075	It is shown that with acidic sample matrix and basic BGE, an efficient and precise approach was designed in which analyte adsorption on the capillary wall was minimized while keeping repeatable peak height, peak area and migration time together with the highest possible enrichment efficiency.
31918849	8	35	theme	dosage	1837:1842	arg1	forms					1844:1848	their pharmaceutical dosage forms	1816:1848	their pharmaceutical dosage forms	1816:1848	The selectivity of the developed method for determination of the studied compounds in their pharmaceutical dosage forms or in the presence of ribavirin (RIB) or elbasvir (ELB), which are other prescribed medications in the treatment regimen of patients with hepatitis C virus infection, is demonstrated.
31918849	1	36	theme	newly-FDA	353:361	arg1	drugs					394:398	the newly-FDA approved hepatitis C antiviral drugs	349:398	the newly-FDA approved hepatitis C antiviral drugs namely	349:405	Based on our previous work with "pseudostationary-ion exchanger sweeping", we use this strategy to develop a sensitive, reliable and robust method for the analysis of the newly-FDA approved hepatitis C antiviral drugs namely; sofosbuvir (SOV), daclatasvir (DAC), ledipasvir (LED) and velpatasvir (VEP) in their pure forms and co-formulated pharmaceutical dosage forms using micellar electrokinetic chromatography (MEKC) as a separation method.
31918849	3	37	theme	sample	971:976	arg1	matrix					978:983	the sample matrix	967:983	the sample matrix	967:983	A special focus was given to optimize the composition of the sample matrix to maintain the solubility of the analytes within the sample zone while gaining additional benefits regarding analyte zone focusing.
31918849	6	38	theme	pharmacopeial	1491:1503	arg1	guidelines					1513:1522	the pharmacopeial and ICH guidelines	1487:1522	guidelines	1513:1522	A full validation study is performed based on the pharmacopeial and ICH guidelines.
31918849	1	39	dep	ledipasvir	445:454	arg1	LED					457:459	LED	457:459	LED	457:459	Based on our previous work with "pseudostationary-ion exchanger sweeping", we use this strategy to develop a sensitive, reliable and robust method for the analysis of the newly-FDA approved hepatitis C antiviral drugs namely; sofosbuvir (SOV), daclatasvir (DAC), ledipasvir (LED) and velpatasvir (VEP) in their pure forms and co-formulated pharmaceutical dosage forms using micellar electrokinetic chromatography (MEKC) as a separation method.
31918849	5	40	theme	enrichment	1348:1357	arg1	effect					1408:1413	the retention factor gradient effect	1378:1413	the retention factor gradient effect (RFGE)	1378:1420	The interplay of other enrichment principles such as the retention factor gradient effect (RFGE) is also discussed.
31918849	5	40	theme	enrichment	1348:1357	arg1	principles					1359:1368	other enrichment principles	1342:1368	other enrichment principles such as the retention factor gradient effect (RFGE)	1342:1420	The interplay of other enrichment principles such as the retention factor gradient effect (RFGE) is also discussed.
31918849	0	41	theme	electrokinetic	61:74	arg1	analysis					92:99	micellar electrokinetic chromatographic analysis	52:99	micellar electrokinetic chromatographic analysis of sofosbuvir	52:113	Optimization of a sensitive and robust strategy for micellar electrokinetic chromatographic analysis of sofosbuvir in combination with its co-formulated hepatitis C antiviral drugs.
31918849	1	42	theme	pure	493:496	arg1	forms					498:502	their pure forms	487:502	their pure forms	487:502	Based on our previous work with "pseudostationary-ion exchanger sweeping", we use this strategy to develop a sensitive, reliable and robust method for the analysis of the newly-FDA approved hepatitis C antiviral drugs namely; sofosbuvir (SOV), daclatasvir (DAC), ledipasvir (LED) and velpatasvir (VEP) in their pure forms and co-formulated pharmaceutical dosage forms using micellar electrokinetic chromatography (MEKC) as a separation method.
31918849	3	43	theme	matrix	978:983	arg1	composition					952:962	the composition	948:962	the composition of the sample matrix to maintain the solubility of the analytes within the sample zone while gaining additional benefits regarding analyte zone focusing	948:1115	A special focus was given to optimize the composition of the sample matrix to maintain the solubility of the analytes within the sample zone while gaining additional benefits regarding analyte zone focusing.
31918849	9	44	theme	possible	2296:2303	arg1	efficiency					2316:2325	the highest possible enrichment efficiency	2284:2325	the highest possible enrichment efficiency	2284:2325	It is shown that with acidic sample matrix and basic BGE, an efficient and precise approach was designed in which analyte adsorption on the capillary wall was minimized while keeping repeatable peak height, peak area and migration time together with the highest possible enrichment efficiency.
31918849	1	45	from	velpatasvir	466:476	arg1	forms					544:548	co-formulated pharmaceutical dosage forms	508:548	co-formulated pharmaceutical dosage forms	508:548	Based on our previous work with "pseudostationary-ion exchanger sweeping", we use this strategy to develop a sensitive, reliable and robust method for the analysis of the newly-FDA approved hepatitis C antiviral drugs namely; sofosbuvir (SOV), daclatasvir (DAC), ledipasvir (LED) and velpatasvir (VEP) in their pure forms and co-formulated pharmaceutical dosage forms using micellar electrokinetic chromatography (MEKC) as a separation method.
31918849	1	45	from	velpatasvir	466:476	arg1	forms					498:502	their pure forms	487:502	their pure forms	487:502	Based on our previous work with "pseudostationary-ion exchanger sweeping", we use this strategy to develop a sensitive, reliable and robust method for the analysis of the newly-FDA approved hepatitis C antiviral drugs namely; sofosbuvir (SOV), daclatasvir (DAC), ledipasvir (LED) and velpatasvir (VEP) in their pure forms and co-formulated pharmaceutical dosage forms using micellar electrokinetic chromatography (MEKC) as a separation method.
31918849	1	46	dep	analysis	337:344	arg1	velpatasvir					466:476	velpatasvir	466:476	velpatasvir	466:476	Based on our previous work with "pseudostationary-ion exchanger sweeping", we use this strategy to develop a sensitive, reliable and robust method for the analysis of the newly-FDA approved hepatitis C antiviral drugs namely; sofosbuvir (SOV), daclatasvir (DAC), ledipasvir (LED) and velpatasvir (VEP) in their pure forms and co-formulated pharmaceutical dosage forms using micellar electrokinetic chromatography (MEKC) as a separation method.
31918849	1	46	dep	analysis	337:344	arg1	SOV					420:422	SOV	420:422	SOV	420:422	Based on our previous work with "pseudostationary-ion exchanger sweeping", we use this strategy to develop a sensitive, reliable and robust method for the analysis of the newly-FDA approved hepatitis C antiviral drugs namely; sofosbuvir (SOV), daclatasvir (DAC), ledipasvir (LED) and velpatasvir (VEP) in their pure forms and co-formulated pharmaceutical dosage forms using micellar electrokinetic chromatography (MEKC) as a separation method.
31918849	1	46	dep	analysis	337:344	arg1	VEP					479:481	VEP	479:481	VEP	479:481	Based on our previous work with "pseudostationary-ion exchanger sweeping", we use this strategy to develop a sensitive, reliable and robust method for the analysis of the newly-FDA approved hepatitis C antiviral drugs namely; sofosbuvir (SOV), daclatasvir (DAC), ledipasvir (LED) and velpatasvir (VEP) in their pure forms and co-formulated pharmaceutical dosage forms using micellar electrokinetic chromatography (MEKC) as a separation method.
31918849	1	46	dep	analysis	337:344	arg1	ledipasvir					445:454	ledipasvir	445:454	ledipasvir (LED)	445:460	Based on our previous work with "pseudostationary-ion exchanger sweeping", we use this strategy to develop a sensitive, reliable and robust method for the analysis of the newly-FDA approved hepatitis C antiviral drugs namely; sofosbuvir (SOV), daclatasvir (DAC), ledipasvir (LED) and velpatasvir (VEP) in their pure forms and co-formulated pharmaceutical dosage forms using micellar electrokinetic chromatography (MEKC) as a separation method.
31918849	1	46	dep	analysis	337:344	arg1	daclatasvir					426:436	daclatasvir	426:436	daclatasvir (DAC)	426:442	Based on our previous work with "pseudostationary-ion exchanger sweeping", we use this strategy to develop a sensitive, reliable and robust method for the analysis of the newly-FDA approved hepatitis C antiviral drugs namely; sofosbuvir (SOV), daclatasvir (DAC), ledipasvir (LED) and velpatasvir (VEP) in their pure forms and co-formulated pharmaceutical dosage forms using micellar electrokinetic chromatography (MEKC) as a separation method.
31918849	1	46	dep	analysis	337:344	arg1	sofosbuvir					408:417	sofosbuvir	408:417	sofosbuvir (SOV)	408:423	Based on our previous work with "pseudostationary-ion exchanger sweeping", we use this strategy to develop a sensitive, reliable and robust method for the analysis of the newly-FDA approved hepatitis C antiviral drugs namely; sofosbuvir (SOV), daclatasvir (DAC), ledipasvir (LED) and velpatasvir (VEP) in their pure forms and co-formulated pharmaceutical dosage forms using micellar electrokinetic chromatography (MEKC) as a separation method.
31918849	1	46	dep	analysis	337:344	arg1	DAC					439:441	DAC	439:441	DAC	439:441	Based on our previous work with "pseudostationary-ion exchanger sweeping", we use this strategy to develop a sensitive, reliable and robust method for the analysis of the newly-FDA approved hepatitis C antiviral drugs namely; sofosbuvir (SOV), daclatasvir (DAC), ledipasvir (LED) and velpatasvir (VEP) in their pure forms and co-formulated pharmaceutical dosage forms using micellar electrokinetic chromatography (MEKC) as a separation method.
31918849	9	47	theme	basic	2081:2085	arg1	BGE					2087:2089	basic BGE	2081:2089	basic BGE	2081:2089	It is shown that with acidic sample matrix and basic BGE, an efficient and precise approach was designed in which analyte adsorption on the capillary wall was minimized while keeping repeatable peak height, peak area and migration time together with the highest possible enrichment efficiency.
31918849	1	48	theme	pharmaceutical	522:535	arg1	forms					544:548	co-formulated pharmaceutical dosage forms	508:548	co-formulated pharmaceutical dosage forms	508:548	Based on our previous work with "pseudostationary-ion exchanger sweeping", we use this strategy to develop a sensitive, reliable and robust method for the analysis of the newly-FDA approved hepatitis C antiviral drugs namely; sofosbuvir (SOV), daclatasvir (DAC), ledipasvir (LED) and velpatasvir (VEP) in their pure forms and co-formulated pharmaceutical dosage forms using micellar electrokinetic chromatography (MEKC) as a separation method.
31918849	7	49	theme	detection	1712:1720	arg1	method					1722:1727	a detection method	1710:1727	a detection method	1710:1727	The obtained limits of detection and quantitation are as low as 0.63 and 1.3 μg mL-1; respectively for SOV and DAC and 1.3 and 2.5 μg mL-1; respectively for LED and VEP using UV-DAD as a detection method.
31918849	7	49	theme	detection	1712:1720	arg1	UV-DAD					1700:1705	UV-DAD	1700:1705	UV-DAD	1700:1705	The obtained limits of detection and quantitation are as low as 0.63 and 1.3 μg mL-1; respectively for SOV and DAC and 1.3 and 2.5 μg mL-1; respectively for LED and VEP using UV-DAD as a detection method.
31918849	5	50	theme	factor	1392:1397	arg1	effect					1408:1413	the retention factor gradient effect	1378:1413	the retention factor gradient effect (RFGE)	1378:1420	The interplay of other enrichment principles such as the retention factor gradient effect (RFGE) is also discussed.
31918849	5	50	theme	factor	1392:1397	arg1	RFGE					1416:1419	RFGE	1416:1419	RFGE	1416:1419	The interplay of other enrichment principles such as the retention factor gradient effect (RFGE) is also discussed.
31918849	9	51	theme	efficient	2095:2103	arg1	approach					2117:2124	an efficient and precise approach	2092:2124	an efficient and precise approach	2092:2124	It is shown that with acidic sample matrix and basic BGE, an efficient and precise approach was designed in which analyte adsorption on the capillary wall was minimized while keeping repeatable peak height, peak area and migration time together with the highest possible enrichment efficiency.
31918849	0	52	theme	strategy	39:46	arg1	Optimization					0:11	Optimization	0:11	Optimization of a sensitive and robust strategy for micellar electrokinetic chromatographic analysis of sofosbuvir in combination with its co-formulated hepatitis C antiviral drugs.	0:180	Optimization of a sensitive and robust strategy for micellar electrokinetic chromatographic analysis of sofosbuvir in combination with its co-formulated hepatitis C antiviral drugs.
31918849	3	53	theme	analytes	1019:1026	arg1	solubility					1001:1010	the solubility	997:1010	the solubility of the analytes within the sample zone	997:1049	A special focus was given to optimize the composition of the sample matrix to maintain the solubility of the analytes within the sample zone while gaining additional benefits regarding analyte zone focusing.
31918849	2	54	theme	basic	744:748	arg1	electrolyte					761:771	a basic background electrolyte	742:771	a basic background electrolyte (BGE) composed of 25 mmol L-1 SDS + 20% (v/v)	742:817	For the first time, a successful separation of all the investigated compounds was achieved in less than 8 min using a basic background electrolyte (BGE) composed of 25 mmol L-1 SDS + 20% (v/v) ACN (acetonitrile) in 10 mmol L-1 disodium tetraborate buffer (final apparent pH is 9.90).
31918849	2	54	theme	basic	744:748	arg1	BGE					774:776	BGE	774:776	BGE	774:776	For the first time, a successful separation of all the investigated compounds was achieved in less than 8 min using a basic background electrolyte (BGE) composed of 25 mmol L-1 SDS + 20% (v/v) ACN (acetonitrile) in 10 mmol L-1 disodium tetraborate buffer (final apparent pH is 9.90).
31918849	9	55	theme	precise	2109:2115	arg1	approach					2117:2124	an efficient and precise approach	2092:2124	an efficient and precise approach	2092:2124	It is shown that with acidic sample matrix and basic BGE, an efficient and precise approach was designed in which analyte adsorption on the capillary wall was minimized while keeping repeatable peak height, peak area and migration time together with the highest possible enrichment efficiency.
31918849	1	56	theme	exchanger	236:244	arg1	"					254:254	"pseudostationary-ion exchanger sweeping"	214:254	"pseudostationary-ion exchanger sweeping"	214:254	Based on our previous work with "pseudostationary-ion exchanger sweeping", we use this strategy to develop a sensitive, reliable and robust method for the analysis of the newly-FDA approved hepatitis C antiviral drugs namely; sofosbuvir (SOV), daclatasvir (DAC), ledipasvir (LED) and velpatasvir (VEP) in their pure forms and co-formulated pharmaceutical dosage forms using micellar electrokinetic chromatography (MEKC) as a separation method.
31918849	8	57	theme	prescribed	1923:1932	arg1	selectivity					1734:1744	The selectivity	1730:1744	The selectivity	1730:1744	The selectivity of the developed method for determination of the studied compounds in their pharmaceutical dosage forms or in the presence of ribavirin (RIB) or elbasvir (ELB), which are other prescribed medications in the treatment regimen of patients with hepatitis C virus infection, is demonstrated.
31918849	8	57	theme	prescribed	1923:1932	arg1	medications					1934:1944	other prescribed medications	1917:1944	other prescribed medications in the treatment regimen of patients with hepatitis C virus infection	1917:2014	The selectivity of the developed method for determination of the studied compounds in their pharmaceutical dosage forms or in the presence of ribavirin (RIB) or elbasvir (ELB), which are other prescribed medications in the treatment regimen of patients with hepatitis C virus infection, is demonstrated.
31918849	4	58	theme	phosphoric	1146:1155	arg1	acid					1157:1160	phosphoric acid	1146:1160	phosphoric acid (as a sample matrix)	1146:1181	It was found that replacing phosphoric acid (as a sample matrix) with a zwitterionic/isoelectric buffering compound (L-glutamic acid) has a substantial positive impact on the obtained enrichment efficiency.
31918849	8	59	with	patients	1974:1981	arg1	infection					2006:2014	hepatitis C virus infection	1988:2014	hepatitis C virus infection	1988:2014	The selectivity of the developed method for determination of the studied compounds in their pharmaceutical dosage forms or in the presence of ribavirin (RIB) or elbasvir (ELB), which are other prescribed medications in the treatment regimen of patients with hepatitis C virus infection, is demonstrated.
31918849	1	60	from	daclatasvir	426:436	arg1	forms					544:548	co-formulated pharmaceutical dosage forms	508:548	co-formulated pharmaceutical dosage forms	508:548	Based on our previous work with "pseudostationary-ion exchanger sweeping", we use this strategy to develop a sensitive, reliable and robust method for the analysis of the newly-FDA approved hepatitis C antiviral drugs namely; sofosbuvir (SOV), daclatasvir (DAC), ledipasvir (LED) and velpatasvir (VEP) in their pure forms and co-formulated pharmaceutical dosage forms using micellar electrokinetic chromatography (MEKC) as a separation method.
31918849	1	60	from	daclatasvir	426:436	arg1	forms					498:502	their pure forms	487:502	their pure forms	487:502	Based on our previous work with "pseudostationary-ion exchanger sweeping", we use this strategy to develop a sensitive, reliable and robust method for the analysis of the newly-FDA approved hepatitis C antiviral drugs namely; sofosbuvir (SOV), daclatasvir (DAC), ledipasvir (LED) and velpatasvir (VEP) in their pure forms and co-formulated pharmaceutical dosage forms using micellar electrokinetic chromatography (MEKC) as a separation method.
31918849	4	61	theme	zwitterionic/isoelectric	1190:1213	arg1	compound					1225:1232	a zwitterionic/isoelectric buffering compound	1188:1232	a zwitterionic/isoelectric buffering compound (L-glutamic acid)	1188:1250	It was found that replacing phosphoric acid (as a sample matrix) with a zwitterionic/isoelectric buffering compound (L-glutamic acid) has a substantial positive impact on the obtained enrichment efficiency.
31918849	4	61	theme	zwitterionic/isoelectric	1190:1213	arg1	acid					1246:1249	L-glutamic acid	1235:1249	L-glutamic acid	1235:1249	It was found that replacing phosphoric acid (as a sample matrix) with a zwitterionic/isoelectric buffering compound (L-glutamic acid) has a substantial positive impact on the obtained enrichment efficiency.
31918849	8	62	theme	pharmaceutical	1822:1835	arg1	forms					1844:1848	their pharmaceutical dosage forms	1816:1848	their pharmaceutical dosage forms	1816:1848	The selectivity of the developed method for determination of the studied compounds in their pharmaceutical dosage forms or in the presence of ribavirin (RIB) or elbasvir (ELB), which are other prescribed medications in the treatment regimen of patients with hepatitis C virus infection, is demonstrated.
31918849	8	63	from	medications	1934:1944	arg1	regimen					1963:1969	the treatment regimen	1949:1969	the treatment regimen of patients with hepatitis C virus infection	1949:2014	The selectivity of the developed method for determination of the studied compounds in their pharmaceutical dosage forms or in the presence of ribavirin (RIB) or elbasvir (ELB), which are other prescribed medications in the treatment regimen of patients with hepatitis C virus infection, is demonstrated.
31918849	8	64	theme	treatment	1953:1961	arg1	regimen					1963:1969	the treatment regimen	1949:1969	the treatment regimen of patients with hepatitis C virus infection	1949:2014	The selectivity of the developed method for determination of the studied compounds in their pharmaceutical dosage forms or in the presence of ribavirin (RIB) or elbasvir (ELB), which are other prescribed medications in the treatment regimen of patients with hepatitis C virus infection, is demonstrated.
31918849	8	65	from	determination	1774:1786	arg1	presence					1860:1867	the presence	1856:1867	the presence of ribavirin (RIB) or elbasvir (ELB)	1856:1904	The selectivity of the developed method for determination of the studied compounds in their pharmaceutical dosage forms or in the presence of ribavirin (RIB) or elbasvir (ELB), which are other prescribed medications in the treatment regimen of patients with hepatitis C virus infection, is demonstrated.
31918849	8	65	from	determination	1774:1786	arg1	forms					1844:1848	their pharmaceutical dosage forms	1816:1848	their pharmaceutical dosage forms	1816:1848	The selectivity of the developed method for determination of the studied compounds in their pharmaceutical dosage forms or in the presence of ribavirin (RIB) or elbasvir (ELB), which are other prescribed medications in the treatment regimen of patients with hepatitis C virus infection, is demonstrated.
31918849	9	66	theme	analyte	2148:2154	arg1	adsorption					2156:2165	analyte adsorption	2148:2165	analyte adsorption on the capillary wall	2148:2187	It is shown that with acidic sample matrix and basic BGE, an efficient and precise approach was designed in which analyte adsorption on the capillary wall was minimized while keeping repeatable peak height, peak area and migration time together with the highest possible enrichment efficiency.
31918849	9	67	from	adsorption	2156:2165	arg1	wall					2184:2187	the capillary wall	2170:2187	the capillary wall	2170:2187	It is shown that with acidic sample matrix and basic BGE, an efficient and precise approach was designed in which analyte adsorption on the capillary wall was minimized while keeping repeatable peak height, peak area and migration time together with the highest possible enrichment efficiency.
31918849	2	68	theme	L-1	799:801	arg1	v/v					814:816	v/v	814:816	v/v	814:816	For the first time, a successful separation of all the investigated compounds was achieved in less than 8 min using a basic background electrolyte (BGE) composed of 25 mmol L-1 SDS + 20% (v/v) ACN (acetonitrile) in 10 mmol L-1 disodium tetraborate buffer (final apparent pH is 9.90).
31918849	2	68	theme	L-1	799:801	arg1	%					811:811	25 mmol L-1 SDS + 20%	791:811	25 mmol L-1 SDS + 20% (v/v)	791:817	For the first time, a successful separation of all the investigated compounds was achieved in less than 8 min using a basic background electrolyte (BGE) composed of 25 mmol L-1 SDS + 20% (v/v) ACN (acetonitrile) in 10 mmol L-1 disodium tetraborate buffer (final apparent pH is 9.90).
31918849	2	69	theme	investigated	681:692	arg1	compounds					694:702	all the investigated compounds	673:702	all the investigated compounds	673:702	For the first time, a successful separation of all the investigated compounds was achieved in less than 8 min using a basic background electrolyte (BGE) composed of 25 mmol L-1 SDS + 20% (v/v) ACN (acetonitrile) in 10 mmol L-1 disodium tetraborate buffer (final apparent pH is 9.90).
31918849	4	70	from	impact	1279:1284	arg1	efficiency					1313:1322	the obtained enrichment efficiency	1289:1322	the obtained enrichment efficiency	1289:1322	It was found that replacing phosphoric acid (as a sample matrix) with a zwitterionic/isoelectric buffering compound (L-glutamic acid) has a substantial positive impact on the obtained enrichment efficiency.
31918849	8	71	theme	compounds	1803:1811	arg1	determination					1774:1786	determination	1774:1786	determination of the studied compounds in their pharmaceutical dosage forms or in the presence of ribavirin (RIB) or elbasvir (ELB)	1774:1904	The selectivity of the developed method for determination of the studied compounds in their pharmaceutical dosage forms or in the presence of ribavirin (RIB) or elbasvir (ELB), which are other prescribed medications in the treatment regimen of patients with hepatitis C virus infection, is demonstrated.
31918849	9	72	theme	capillary	2174:2182	arg1	wall					2184:2187	the capillary wall	2170:2187	the capillary wall	2170:2187	It is shown that with acidic sample matrix and basic BGE, an efficient and precise approach was designed in which analyte adsorption on the capillary wall was minimized while keeping repeatable peak height, peak area and migration time together with the highest possible enrichment efficiency.
31918849	3	73	theme	focusing	1108:1115	arg1	zone					1103:1106	analyte zone focusing	1095:1115	analyte zone focusing	1095:1115	A special focus was given to optimize the composition of the sample matrix to maintain the solubility of the analytes within the sample zone while gaining additional benefits regarding analyte zone focusing.
31918849	8	74	theme	C	1998:1998	arg1	virus					2000:2004	hepatitis C virus	1988:2004	hepatitis C virus infection	1988:2014	The selectivity of the developed method for determination of the studied compounds in their pharmaceutical dosage forms or in the presence of ribavirin (RIB) or elbasvir (ELB), which are other prescribed medications in the treatment regimen of patients with hepatitis C virus infection, is demonstrated.
31918849	8	75	theme	studied	1795:1801	arg1	compounds					1803:1811	the studied compounds	1791:1811	the studied compounds	1791:1811	The selectivity of the developed method for determination of the studied compounds in their pharmaceutical dosage forms or in the presence of ribavirin (RIB) or elbasvir (ELB), which are other prescribed medications in the treatment regimen of patients with hepatitis C virus infection, is demonstrated.
31918849	3	76	theme	analyte	1095:1101	arg1	zone					1103:1106	analyte zone focusing	1095:1115	analyte zone focusing	1095:1115	A special focus was given to optimize the composition of the sample matrix to maintain the solubility of the analytes within the sample zone while gaining additional benefits regarding analyte zone focusing.
31918849	4	77	theme	positive	1270:1277	arg1	impact					1279:1284	a substantial positive impact	1256:1284	a substantial positive impact on the obtained enrichment efficiency	1256:1322	It was found that replacing phosphoric acid (as a sample matrix) with a zwitterionic/isoelectric buffering compound (L-glutamic acid) has a substantial positive impact on the obtained enrichment efficiency.
31918849	0	78	theme	hepatitis	153:161	arg1	drugs					175:179	its co-formulated hepatitis C antiviral drugs	135:179	its co-formulated hepatitis C antiviral drugs	135:179	Optimization of a sensitive and robust strategy for micellar electrokinetic chromatographic analysis of sofosbuvir in combination with its co-formulated hepatitis C antiviral drugs.
31918849	1	79	theme	C	382:382	arg1	drugs					394:398	the newly-FDA approved hepatitis C antiviral drugs	349:398	the newly-FDA approved hepatitis C antiviral drugs namely	349:405	Based on our previous work with "pseudostationary-ion exchanger sweeping", we use this strategy to develop a sensitive, reliable and robust method for the analysis of the newly-FDA approved hepatitis C antiviral drugs namely; sofosbuvir (SOV), daclatasvir (DAC), ledipasvir (LED) and velpatasvir (VEP) in their pure forms and co-formulated pharmaceutical dosage forms using micellar electrokinetic chromatography (MEKC) as a separation method.
31918849	4	80	theme	L-glutamic	1235:1244	arg1	compound					1225:1232	a zwitterionic/isoelectric buffering compound	1188:1232	a zwitterionic/isoelectric buffering compound (L-glutamic acid)	1188:1250	It was found that replacing phosphoric acid (as a sample matrix) with a zwitterionic/isoelectric buffering compound (L-glutamic acid) has a substantial positive impact on the obtained enrichment efficiency.
31918849	4	80	theme	L-glutamic	1235:1244	arg1	acid					1246:1249	L-glutamic acid	1235:1249	L-glutamic acid	1235:1249	It was found that replacing phosphoric acid (as a sample matrix) with a zwitterionic/isoelectric buffering compound (L-glutamic acid) has a substantial positive impact on the obtained enrichment efficiency.
31918849	0	81	theme	antiviral	165:173	arg1	drugs					175:179	its co-formulated hepatitis C antiviral drugs	135:179	its co-formulated hepatitis C antiviral drugs	135:179	Optimization of a sensitive and robust strategy for micellar electrokinetic chromatographic analysis of sofosbuvir in combination with its co-formulated hepatitis C antiviral drugs.
31918849	1	82	theme	drugs	394:398	arg1	analysis					337:344	the analysis	333:344	the analysis of the newly-FDA approved hepatitis C antiviral drugs namely; sofosbuvir (SOV), daclatasvir (DAC), ledipasvir (LED) and velpatasvir (VEP) in their pure forms and co-formulated pharmaceutical dosage forms	333:548	Based on our previous work with "pseudostationary-ion exchanger sweeping", we use this strategy to develop a sensitive, reliable and robust method for the analysis of the newly-FDA approved hepatitis C antiviral drugs namely; sofosbuvir (SOV), daclatasvir (DAC), ledipasvir (LED) and velpatasvir (VEP) in their pure forms and co-formulated pharmaceutical dosage forms using micellar electrokinetic chromatography (MEKC) as a separation method.
31918849	9	83	theme	repeatable	2217:2226	arg1	time					2265:2268	migration time	2255:2268	migration time	2255:2268	It is shown that with acidic sample matrix and basic BGE, an efficient and precise approach was designed in which analyte adsorption on the capillary wall was minimized while keeping repeatable peak height, peak area and migration time together with the highest possible enrichment efficiency.
31918849	9	83	theme	repeatable	2217:2226	arg1	peak					2228:2231	repeatable peak height	2217:2238	repeatable peak height	2217:2238	It is shown that with acidic sample matrix and basic BGE, an efficient and precise approach was designed in which analyte adsorption on the capillary wall was minimized while keeping repeatable peak height, peak area and migration time together with the highest possible enrichment efficiency.
31918849	9	83	theme	repeatable	2217:2226	arg1	area					2246:2249	peak area	2241:2249	peak area	2241:2249	It is shown that with acidic sample matrix and basic BGE, an efficient and precise approach was designed in which analyte adsorption on the capillary wall was minimized while keeping repeatable peak height, peak area and migration time together with the highest possible enrichment efficiency.
31918849	2	84	theme	L-1	849:851	arg1	buffer					874:879	10 mmol L-1 disodium tetraborate buffer	841:879	10 mmol L-1 disodium tetraborate buffer (final apparent pH is 9.90)	841:907	For the first time, a successful separation of all the investigated compounds was achieved in less than 8 min using a basic background electrolyte (BGE) composed of 25 mmol L-1 SDS + 20% (v/v) ACN (acetonitrile) in 10 mmol L-1 disodium tetraborate buffer (final apparent pH is 9.90).
31918849	1	85	theme	dosage	537:542	arg1	forms					544:548	co-formulated pharmaceutical dosage forms	508:548	co-formulated pharmaceutical dosage forms	508:548	Based on our previous work with "pseudostationary-ion exchanger sweeping", we use this strategy to develop a sensitive, reliable and robust method for the analysis of the newly-FDA approved hepatitis C antiviral drugs namely; sofosbuvir (SOV), daclatasvir (DAC), ledipasvir (LED) and velpatasvir (VEP) in their pure forms and co-formulated pharmaceutical dosage forms using micellar electrokinetic chromatography (MEKC) as a separation method.
31918849	6	86	theme	validation	1448:1457	arg1	study					1459:1463	A full validation study	1441:1463	A full validation study	1441:1463	A full validation study is performed based on the pharmacopeial and ICH guidelines.
31918849	1	87	from	sofosbuvir	408:417	arg1	forms					544:548	co-formulated pharmaceutical dosage forms	508:548	co-formulated pharmaceutical dosage forms	508:548	Based on our previous work with "pseudostationary-ion exchanger sweeping", we use this strategy to develop a sensitive, reliable and robust method for the analysis of the newly-FDA approved hepatitis C antiviral drugs namely; sofosbuvir (SOV), daclatasvir (DAC), ledipasvir (LED) and velpatasvir (VEP) in their pure forms and co-formulated pharmaceutical dosage forms using micellar electrokinetic chromatography (MEKC) as a separation method.
31918849	1	87	from	sofosbuvir	408:417	arg1	forms					498:502	their pure forms	487:502	their pure forms	487:502	Based on our previous work with "pseudostationary-ion exchanger sweeping", we use this strategy to develop a sensitive, reliable and robust method for the analysis of the newly-FDA approved hepatitis C antiviral drugs namely; sofosbuvir (SOV), daclatasvir (DAC), ledipasvir (LED) and velpatasvir (VEP) in their pure forms and co-formulated pharmaceutical dosage forms using micellar electrokinetic chromatography (MEKC) as a separation method.
31918849	9	88	theme	peak	2241:2244	arg1	peak					2228:2231	repeatable peak height	2217:2238	repeatable peak height	2217:2238	It is shown that with acidic sample matrix and basic BGE, an efficient and precise approach was designed in which analyte adsorption on the capillary wall was minimized while keeping repeatable peak height, peak area and migration time together with the highest possible enrichment efficiency.
31918849	9	88	theme	peak	2241:2244	arg1	area					2246:2249	peak area	2241:2249	peak area	2241:2249	It is shown that with acidic sample matrix and basic BGE, an efficient and precise approach was designed in which analyte adsorption on the capillary wall was minimized while keeping repeatable peak height, peak area and migration time together with the highest possible enrichment efficiency.
31918849	1	89	theme	electrokinetic	565:578	arg1	chromatography					580:593	micellar electrokinetic chromatography	556:593	micellar electrokinetic chromatography (MEKC)	556:600	Based on our previous work with "pseudostationary-ion exchanger sweeping", we use this strategy to develop a sensitive, reliable and robust method for the analysis of the newly-FDA approved hepatitis C antiviral drugs namely; sofosbuvir (SOV), daclatasvir (DAC), ledipasvir (LED) and velpatasvir (VEP) in their pure forms and co-formulated pharmaceutical dosage forms using micellar electrokinetic chromatography (MEKC) as a separation method.
31918849	1	89	theme	electrokinetic	565:578	arg1	method					618:623	a separation method	605:623	a separation method	605:623	Based on our previous work with "pseudostationary-ion exchanger sweeping", we use this strategy to develop a sensitive, reliable and robust method for the analysis of the newly-FDA approved hepatitis C antiviral drugs namely; sofosbuvir (SOV), daclatasvir (DAC), ledipasvir (LED) and velpatasvir (VEP) in their pure forms and co-formulated pharmaceutical dosage forms using micellar electrokinetic chromatography (MEKC) as a separation method.
31918849	1	89	theme	electrokinetic	565:578	arg1	MEKC					596:599	MEKC	596:599	MEKC	596:599	Based on our previous work with "pseudostationary-ion exchanger sweeping", we use this strategy to develop a sensitive, reliable and robust method for the analysis of the newly-FDA approved hepatitis C antiviral drugs namely; sofosbuvir (SOV), daclatasvir (DAC), ledipasvir (LED) and velpatasvir (VEP) in their pure forms and co-formulated pharmaceutical dosage forms using micellar electrokinetic chromatography (MEKC) as a separation method.
31918849	2	90	theme	disodium	853:860	arg1	buffer					874:879	10 mmol L-1 disodium tetraborate buffer	841:879	10 mmol L-1 disodium tetraborate buffer (final apparent pH is 9.90)	841:907	For the first time, a successful separation of all the investigated compounds was achieved in less than 8 min using a basic background electrolyte (BGE) composed of 25 mmol L-1 SDS + 20% (v/v) ACN (acetonitrile) in 10 mmol L-1 disodium tetraborate buffer (final apparent pH is 9.90).
31918849	0	91	theme	sofosbuvir	104:113	arg1	analysis					92:99	micellar electrokinetic chromatographic analysis	52:99	micellar electrokinetic chromatographic analysis of sofosbuvir	52:113	Optimization of a sensitive and robust strategy for micellar electrokinetic chromatographic analysis of sofosbuvir in combination with its co-formulated hepatitis C antiviral drugs.
31918849	5	92	theme	retention	1382:1390	arg1	effect					1408:1413	the retention factor gradient effect	1378:1413	the retention factor gradient effect (RFGE)	1378:1420	The interplay of other enrichment principles such as the retention factor gradient effect (RFGE) is also discussed.
31918849	5	92	theme	retention	1382:1390	arg1	RFGE					1416:1419	RFGE	1416:1419	RFGE	1416:1419	The interplay of other enrichment principles such as the retention factor gradient effect (RFGE) is also discussed.
31918849	4	93	theme	obtained	1293:1300	arg1	efficiency					1313:1322	the obtained enrichment efficiency	1289:1322	the obtained enrichment efficiency	1289:1322	It was found that replacing phosphoric acid (as a sample matrix) with a zwitterionic/isoelectric buffering compound (L-glutamic acid) has a substantial positive impact on the obtained enrichment efficiency.
31918849	2	94	theme	final	882:886	arg1	pH					897:898	final apparent pH	882:898	final apparent pH is 9.90	882:906	For the first time, a successful separation of all the investigated compounds was achieved in less than 8 min using a basic background electrolyte (BGE) composed of 25 mmol L-1 SDS + 20% (v/v) ACN (acetonitrile) in 10 mmol L-1 disodium tetraborate buffer (final apparent pH is 9.90).
31918849	5	95	theme	other	1342:1346	arg1	effect					1408:1413	the retention factor gradient effect	1378:1413	the retention factor gradient effect (RFGE)	1378:1420	The interplay of other enrichment principles such as the retention factor gradient effect (RFGE) is also discussed.
31918849	5	95	theme	other	1342:1346	arg1	principles					1359:1368	other enrichment principles	1342:1368	other enrichment principles such as the retention factor gradient effect (RFGE)	1342:1420	The interplay of other enrichment principles such as the retention factor gradient effect (RFGE) is also discussed.
31918849	9	96	theme	sample	2063:2068	arg1	matrix					2070:2075	acidic sample matrix	2056:2075	acidic sample matrix	2056:2075	It is shown that with acidic sample matrix and basic BGE, an efficient and precise approach was designed in which analyte adsorption on the capillary wall was minimized while keeping repeatable peak height, peak area and migration time together with the highest possible enrichment efficiency.
31918849	1	97	theme	approved	363:370	arg1	drugs					394:398	the newly-FDA approved hepatitis C antiviral drugs	349:398	the newly-FDA approved hepatitis C antiviral drugs namely	349:405	Based on our previous work with "pseudostationary-ion exchanger sweeping", we use this strategy to develop a sensitive, reliable and robust method for the analysis of the newly-FDA approved hepatitis C antiviral drugs namely; sofosbuvir (SOV), daclatasvir (DAC), ledipasvir (LED) and velpatasvir (VEP) in their pure forms and co-formulated pharmaceutical dosage forms using micellar electrokinetic chromatography (MEKC) as a separation method.
31918849	9	98	theme	highest	2288:2294	arg1	efficiency					2316:2325	the highest possible enrichment efficiency	2284:2325	the highest possible enrichment efficiency	2284:2325	It is shown that with acidic sample matrix and basic BGE, an efficient and precise approach was designed in which analyte adsorption on the capillary wall was minimized while keeping repeatable peak height, peak area and migration time together with the highest possible enrichment efficiency.
31918849	8	99	theme	developed	1753:1761	arg1	method					1763:1768	the developed method	1749:1768	the developed method	1749:1768	The selectivity of the developed method for determination of the studied compounds in their pharmaceutical dosage forms or in the presence of ribavirin (RIB) or elbasvir (ELB), which are other prescribed medications in the treatment regimen of patients with hepatitis C virus infection, is demonstrated.
31918849	5	100	theme	principles	1359:1368	arg1	interplay					1329:1337	The interplay	1325:1337	The interplay of other enrichment principles such as the retention factor gradient effect (RFGE)	1325:1420	The interplay of other enrichment principles such as the retention factor gradient effect (RFGE) is also discussed.
31918849	0	101	theme	sensitive	18:26	arg1	strategy					39:46	a sensitive and robust strategy	16:46	a sensitive and robust strategy for micellar electrokinetic chromatographic analysis of sofosbuvir	16:113	Optimization of a sensitive and robust strategy for micellar electrokinetic chromatographic analysis of sofosbuvir in combination with its co-formulated hepatitis C antiviral drugs.
31918849	9	102	theme	enrichment	2305:2314	arg1	efficiency					2316:2325	the highest possible enrichment efficiency	2284:2325	the highest possible enrichment efficiency	2284:2325	It is shown that with acidic sample matrix and basic BGE, an efficient and precise approach was designed in which analyte adsorption on the capillary wall was minimized while keeping repeatable peak height, peak area and migration time together with the highest possible enrichment efficiency.
31918849	1	103	theme	co-formulated	508:520	arg1	forms					544:548	co-formulated pharmaceutical dosage forms	508:548	co-formulated pharmaceutical dosage forms	508:548	Based on our previous work with "pseudostationary-ion exchanger sweeping", we use this strategy to develop a sensitive, reliable and robust method for the analysis of the newly-FDA approved hepatitis C antiviral drugs namely; sofosbuvir (SOV), daclatasvir (DAC), ledipasvir (LED) and velpatasvir (VEP) in their pure forms and co-formulated pharmaceutical dosage forms using micellar electrokinetic chromatography (MEKC) as a separation method.
31918849	0	104	theme	robust	32:37	arg1	strategy					39:46	a sensitive and robust strategy	16:46	a sensitive and robust strategy for micellar electrokinetic chromatographic analysis of sofosbuvir	16:113	Optimization of a sensitive and robust strategy for micellar electrokinetic chromatographic analysis of sofosbuvir in combination with its co-formulated hepatitis C antiviral drugs.
31918849	1	105	theme	sensitive	291:299	arg1	method					322:327	a sensitive, reliable and robust method	289:327	a sensitive, reliable and robust method for the analysis of the newly-FDA approved hepatitis C antiviral drugs namely; sofosbuvir (SOV), daclatasvir (DAC), ledipasvir (LED) and velpatasvir (VEP) in their pure forms and co-formulated pharmaceutical dosage forms	289:548	Based on our previous work with "pseudostationary-ion exchanger sweeping", we use this strategy to develop a sensitive, reliable and robust method for the analysis of the newly-FDA approved hepatitis C antiviral drugs namely; sofosbuvir (SOV), daclatasvir (DAC), ledipasvir (LED) and velpatasvir (VEP) in their pure forms and co-formulated pharmaceutical dosage forms using micellar electrokinetic chromatography (MEKC) as a separation method.
31918849	1	106	with	work	204:207	arg1	"					254:254	"pseudostationary-ion exchanger sweeping"	214:254	"pseudostationary-ion exchanger sweeping"	214:254	Based on our previous work with "pseudostationary-ion exchanger sweeping", we use this strategy to develop a sensitive, reliable and robust method for the analysis of the newly-FDA approved hepatitis C antiviral drugs namely; sofosbuvir (SOV), daclatasvir (DAC), ledipasvir (LED) and velpatasvir (VEP) in their pure forms and co-formulated pharmaceutical dosage forms using micellar electrokinetic chromatography (MEKC) as a separation method.
31918849	7	107	theme	obtained	1529:1536	arg1	limits					1538:1543	The obtained limits	1525:1543	The obtained limits of detection and quantitation	1525:1573	The obtained limits of detection and quantitation are as low as 0.63 and 1.3 μg mL-1; respectively for SOV and DAC and 1.3 and 2.5 μg mL-1; respectively for LED and VEP using UV-DAD as a detection method.
31918849	7	107	theme	obtained	1529:1536	arg1	low					1582:1584	low	1582:1584	low	1582:1584	The obtained limits of detection and quantitation are as low as 0.63 and 1.3 μg mL-1; respectively for SOV and DAC and 1.3 and 2.5 μg mL-1; respectively for LED and VEP using UV-DAD as a detection method.
31918849	1	108	from	ledipasvir	445:454	arg1	forms					544:548	co-formulated pharmaceutical dosage forms	508:548	co-formulated pharmaceutical dosage forms	508:548	Based on our previous work with "pseudostationary-ion exchanger sweeping", we use this strategy to develop a sensitive, reliable and robust method for the analysis of the newly-FDA approved hepatitis C antiviral drugs namely; sofosbuvir (SOV), daclatasvir (DAC), ledipasvir (LED) and velpatasvir (VEP) in their pure forms and co-formulated pharmaceutical dosage forms using micellar electrokinetic chromatography (MEKC) as a separation method.
31918849	1	108	from	ledipasvir	445:454	arg1	forms					498:502	their pure forms	487:502	their pure forms	487:502	Based on our previous work with "pseudostationary-ion exchanger sweeping", we use this strategy to develop a sensitive, reliable and robust method for the analysis of the newly-FDA approved hepatitis C antiviral drugs namely; sofosbuvir (SOV), daclatasvir (DAC), ledipasvir (LED) and velpatasvir (VEP) in their pure forms and co-formulated pharmaceutical dosage forms using micellar electrokinetic chromatography (MEKC) as a separation method.
31918849	2	109	theme	10 mmol	841:847	arg1	buffer					874:879	10 mmol L-1 disodium tetraborate buffer	841:879	10 mmol L-1 disodium tetraborate buffer (final apparent pH is 9.90)	841:907	For the first time, a successful separation of all the investigated compounds was achieved in less than 8 min using a basic background electrolyte (BGE) composed of 25 mmol L-1 SDS + 20% (v/v) ACN (acetonitrile) in 10 mmol L-1 disodium tetraborate buffer (final apparent pH is 9.90).
31918849	4	110	theme	sample	1168:1173	arg1	matrix					1175:1180	a sample matrix	1166:1180	a sample matrix	1166:1180	It was found that replacing phosphoric acid (as a sample matrix) with a zwitterionic/isoelectric buffering compound (L-glutamic acid) has a substantial positive impact on the obtained enrichment efficiency.
31918849	1	111	theme	reliable	302:309	arg1	method					322:327	a sensitive, reliable and robust method	289:327	a sensitive, reliable and robust method for the analysis of the newly-FDA approved hepatitis C antiviral drugs namely; sofosbuvir (SOV), daclatasvir (DAC), ledipasvir (LED) and velpatasvir (VEP) in their pure forms and co-formulated pharmaceutical dosage forms	289:548	Based on our previous work with "pseudostationary-ion exchanger sweeping", we use this strategy to develop a sensitive, reliable and robust method for the analysis of the newly-FDA approved hepatitis C antiviral drugs namely; sofosbuvir (SOV), daclatasvir (DAC), ledipasvir (LED) and velpatasvir (VEP) in their pure forms and co-formulated pharmaceutical dosage forms using micellar electrokinetic chromatography (MEKC) as a separation method.
31918849	8	112	theme	ribavirin	1872:1880	arg1	presence					1860:1867	the presence	1856:1867	the presence of ribavirin (RIB) or elbasvir (ELB)	1856:1904	The selectivity of the developed method for determination of the studied compounds in their pharmaceutical dosage forms or in the presence of ribavirin (RIB) or elbasvir (ELB), which are other prescribed medications in the treatment regimen of patients with hepatitis C virus infection, is demonstrated.
31918849	8	113	theme	other	1917:1921	arg1	selectivity					1734:1744	The selectivity	1730:1744	The selectivity	1730:1744	The selectivity of the developed method for determination of the studied compounds in their pharmaceutical dosage forms or in the presence of ribavirin (RIB) or elbasvir (ELB), which are other prescribed medications in the treatment regimen of patients with hepatitis C virus infection, is demonstrated.
31918849	8	113	theme	other	1917:1921	arg1	medications					1934:1944	other prescribed medications	1917:1944	other prescribed medications in the treatment regimen of patients with hepatitis C virus infection	1917:2014	The selectivity of the developed method for determination of the studied compounds in their pharmaceutical dosage forms or in the presence of ribavirin (RIB) or elbasvir (ELB), which are other prescribed medications in the treatment regimen of patients with hepatitis C virus infection, is demonstrated.
31918849	5	114	theme	gradient	1399:1406	arg1	effect					1408:1413	the retention factor gradient effect	1378:1413	the retention factor gradient effect (RFGE)	1378:1420	The interplay of other enrichment principles such as the retention factor gradient effect (RFGE) is also discussed.
31918849	5	114	theme	gradient	1399:1406	arg1	RFGE					1416:1419	RFGE	1416:1419	RFGE	1416:1419	The interplay of other enrichment principles such as the retention factor gradient effect (RFGE) is also discussed.
31918849	1	115	theme	robust	315:320	arg1	method					322:327	a sensitive, reliable and robust method	289:327	a sensitive, reliable and robust method for the analysis of the newly-FDA approved hepatitis C antiviral drugs namely; sofosbuvir (SOV), daclatasvir (DAC), ledipasvir (LED) and velpatasvir (VEP) in their pure forms and co-formulated pharmaceutical dosage forms	289:548	Based on our previous work with "pseudostationary-ion exchanger sweeping", we use this strategy to develop a sensitive, reliable and robust method for the analysis of the newly-FDA approved hepatitis C antiviral drugs namely; sofosbuvir (SOV), daclatasvir (DAC), ledipasvir (LED) and velpatasvir (VEP) in their pure forms and co-formulated pharmaceutical dosage forms using micellar electrokinetic chromatography (MEKC) as a separation method.
31918849	2	116	theme	25 mmol	791:797	arg1	v/v					814:816	v/v	814:816	v/v	814:816	For the first time, a successful separation of all the investigated compounds was achieved in less than 8 min using a basic background electrolyte (BGE) composed of 25 mmol L-1 SDS + 20% (v/v) ACN (acetonitrile) in 10 mmol L-1 disodium tetraborate buffer (final apparent pH is 9.90).
31918849	2	116	theme	25 mmol	791:797	arg1	%					811:811	25 mmol L-1 SDS + 20%	791:811	25 mmol L-1 SDS + 20% (v/v)	791:817	For the first time, a successful separation of all the investigated compounds was achieved in less than 8 min using a basic background electrolyte (BGE) composed of 25 mmol L-1 SDS + 20% (v/v) ACN (acetonitrile) in 10 mmol L-1 disodium tetraborate buffer (final apparent pH is 9.90).
31918849	7	117	theme	detection	1548:1556	arg1	limits					1538:1543	The obtained limits	1525:1543	The obtained limits of detection and quantitation	1525:1573	The obtained limits of detection and quantitation are as low as 0.63 and 1.3 μg mL-1; respectively for SOV and DAC and 1.3 and 2.5 μg mL-1; respectively for LED and VEP using UV-DAD as a detection method.
31918849	7	117	theme	detection	1548:1556	arg1	low					1582:1584	low	1582:1584	low	1582:1584	The obtained limits of detection and quantitation are as low as 0.63 and 1.3 μg mL-1; respectively for SOV and DAC and 1.3 and 2.5 μg mL-1; respectively for LED and VEP using UV-DAD as a detection method.
31918849	1	118	theme	sweeping	246:253	arg1	"					254:254	"pseudostationary-ion exchanger sweeping"	214:254	"pseudostationary-ion exchanger sweeping"	214:254	Based on our previous work with "pseudostationary-ion exchanger sweeping", we use this strategy to develop a sensitive, reliable and robust method for the analysis of the newly-FDA approved hepatitis C antiviral drugs namely; sofosbuvir (SOV), daclatasvir (DAC), ledipasvir (LED) and velpatasvir (VEP) in their pure forms and co-formulated pharmaceutical dosage forms using micellar electrokinetic chromatography (MEKC) as a separation method.
31918849	3	119	theme	sample	1039:1044	arg1	zone					1046:1049	the sample zone	1035:1049	the sample zone	1035:1049	A special focus was given to optimize the composition of the sample matrix to maintain the solubility of the analytes within the sample zone while gaining additional benefits regarding analyte zone focusing.
31918849	4	120	theme	buffering	1215:1223	arg1	compound					1225:1232	a zwitterionic/isoelectric buffering compound	1188:1232	a zwitterionic/isoelectric buffering compound (L-glutamic acid)	1188:1250	It was found that replacing phosphoric acid (as a sample matrix) with a zwitterionic/isoelectric buffering compound (L-glutamic acid) has a substantial positive impact on the obtained enrichment efficiency.
31918849	4	120	theme	buffering	1215:1223	arg1	acid					1246:1249	L-glutamic acid	1235:1249	L-glutamic acid	1235:1249	It was found that replacing phosphoric acid (as a sample matrix) with a zwitterionic/isoelectric buffering compound (L-glutamic acid) has a substantial positive impact on the obtained enrichment efficiency.
30221225	1	0	from	structure	175:183	arg1	process					233:239	the calcification process	215:239	the calcification process	215:239	Polysaccharides represent a main weight fraction of the intraskeletal organic matrix of corals, but their structure, as well as their function in the calcification process, has been poorly investigated.
30221225	2	1	theme	acid	466:469	arg1	component					432:440	their key component	422:440	their key component	422:440	This communication shows by a combination of techniques (nuclear magnetic resonance, Fourier transform infrared, and monosaccharide composition) that their key component is a 1→3 β-d glucuronic acid polymer and evidences its influence in vitro in the calcification process.
30221225	2	1	theme	acid	466:469	arg1	polymer					471:477	a 1→3 β-d glucuronic acid polymer	445:477	a 1→3 β-d glucuronic acid polymer	445:477	This communication shows by a combination of techniques (nuclear magnetic resonance, Fourier transform infrared, and monosaccharide composition) that their key component is a 1→3 β-d glucuronic acid polymer and evidences its influence in vitro in the calcification process.
30221225	2	2	theme	magnetic	337:344	arg1	resonance					346:354	nuclear magnetic resonance	329:354	nuclear magnetic resonance	329:354	This communication shows by a combination of techniques (nuclear magnetic resonance, Fourier transform infrared, and monosaccharide composition) that their key component is a 1→3 β-d glucuronic acid polymer and evidences its influence in vitro in the calcification process.
30221225	2	2	theme	magnetic	337:344	arg1	techniques					317:326	techniques	317:326	techniques (nuclear magnetic resonance, Fourier transform infrared, and monosaccharide composition)	317:415	This communication shows by a combination of techniques (nuclear magnetic resonance, Fourier transform infrared, and monosaccharide composition) that their key component is a 1→3 β-d glucuronic acid polymer and evidences its influence in vitro in the calcification process.
30221225	2	3	dep	techniques	317:326	arg1	composition					404:414	monosaccharide composition	389:414	monosaccharide composition	389:414	This communication shows by a combination of techniques (nuclear magnetic resonance, Fourier transform infrared, and monosaccharide composition) that their key component is a 1→3 β-d glucuronic acid polymer and evidences its influence in vitro in the calcification process.
30221225	2	3	dep	techniques	317:326	arg1	Fourier					357:363	Fourier	357:363	Fourier transform infrared	357:382	This communication shows by a combination of techniques (nuclear magnetic resonance, Fourier transform infrared, and monosaccharide composition) that their key component is a 1→3 β-d glucuronic acid polymer and evidences its influence in vitro in the calcification process.
30221225	2	3	dep	techniques	317:326	arg1	techniques					317:326	techniques	317:326	techniques (nuclear magnetic resonance, Fourier transform infrared, and monosaccharide composition)	317:415	This communication shows by a combination of techniques (nuclear magnetic resonance, Fourier transform infrared, and monosaccharide composition) that their key component is a 1→3 β-d glucuronic acid polymer and evidences its influence in vitro in the calcification process.
30221225	2	3	dep	techniques	317:326	arg1	resonance					346:354	nuclear magnetic resonance	329:354	nuclear magnetic resonance	329:354	This communication shows by a combination of techniques (nuclear magnetic resonance, Fourier transform infrared, and monosaccharide composition) that their key component is a 1→3 β-d glucuronic acid polymer and evidences its influence in vitro in the calcification process.
30221225	2	4	dep	Fourier	357:363	arg1	transform					365:373	transform	365:373	transform infrared	365:382	This communication shows by a combination of techniques (nuclear magnetic resonance, Fourier transform infrared, and monosaccharide composition) that their key component is a 1→3 β-d glucuronic acid polymer and evidences its influence in vitro in the calcification process.
30221225	0	5	theme	Coral	32:36	arg1	Polysaccharides					52:66	Stony Coral Intraskeletal Polysaccharides	26:66	Stony Coral Intraskeletal Polysaccharides	26:66	Structure and Function of Stony Coral Intraskeletal Polysaccharides.
30221225	1	6	theme	main	97:100	arg1	fraction					109:116	a main weight fraction	95:116	a main weight fraction of the intraskeletal organic matrix of corals	95:162	Polysaccharides represent a main weight fraction of the intraskeletal organic matrix of corals, but their structure, as well as their function in the calcification process, has been poorly investigated.
30221225	1	7	theme	weight	102:107	arg1	fraction					109:116	a main weight fraction	95:116	a main weight fraction of the intraskeletal organic matrix of corals	95:162	Polysaccharides represent a main weight fraction of the intraskeletal organic matrix of corals, but their structure, as well as their function in the calcification process, has been poorly investigated.
30221225	2	8	theme	key	428:430	arg1	component					432:440	their key component	422:440	their key component	422:440	This communication shows by a combination of techniques (nuclear magnetic resonance, Fourier transform infrared, and monosaccharide composition) that their key component is a 1→3 β-d glucuronic acid polymer and evidences its influence in vitro in the calcification process.
30221225	2	8	theme	key	428:430	arg1	polymer					471:477	a 1→3 β-d glucuronic acid polymer	445:477	a 1→3 β-d glucuronic acid polymer	445:477	This communication shows by a combination of techniques (nuclear magnetic resonance, Fourier transform infrared, and monosaccharide composition) that their key component is a 1→3 β-d glucuronic acid polymer and evidences its influence in vitro in the calcification process.
30221225	2	9	theme	calcification	523:535	arg1	process					537:543	the calcification process	519:543	the calcification process	519:543	This communication shows by a combination of techniques (nuclear magnetic resonance, Fourier transform infrared, and monosaccharide composition) that their key component is a 1→3 β-d glucuronic acid polymer and evidences its influence in vitro in the calcification process.
30221225	2	10	from	polymer	471:477	arg1	process					537:543	the calcification process	519:543	the calcification process	519:543	This communication shows by a combination of techniques (nuclear magnetic resonance, Fourier transform infrared, and monosaccharide composition) that their key component is a 1→3 β-d glucuronic acid polymer and evidences its influence in vitro in the calcification process.
30221225	2	11	theme	glucuronic	455:464	arg1	component					432:440	their key component	422:440	their key component	422:440	This communication shows by a combination of techniques (nuclear magnetic resonance, Fourier transform infrared, and monosaccharide composition) that their key component is a 1→3 β-d glucuronic acid polymer and evidences its influence in vitro in the calcification process.
30221225	2	11	theme	glucuronic	455:464	arg1	polymer					471:477	a 1→3 β-d glucuronic acid polymer	445:477	a 1→3 β-d glucuronic acid polymer	445:477	This communication shows by a combination of techniques (nuclear magnetic resonance, Fourier transform infrared, and monosaccharide composition) that their key component is a 1→3 β-d glucuronic acid polymer and evidences its influence in vitro in the calcification process.
30221225	2	12	theme	β-d	451:453	arg1	component					432:440	their key component	422:440	their key component	422:440	This communication shows by a combination of techniques (nuclear magnetic resonance, Fourier transform infrared, and monosaccharide composition) that their key component is a 1→3 β-d glucuronic acid polymer and evidences its influence in vitro in the calcification process.
30221225	2	12	theme	β-d	451:453	arg1	polymer					471:477	a 1→3 β-d glucuronic acid polymer	445:477	a 1→3 β-d glucuronic acid polymer	445:477	This communication shows by a combination of techniques (nuclear magnetic resonance, Fourier transform infrared, and monosaccharide composition) that their key component is a 1→3 β-d glucuronic acid polymer and evidences its influence in vitro in the calcification process.
30221225	0	13	theme	Stony	26:30	arg1	Polysaccharides					52:66	Stony Coral Intraskeletal Polysaccharides	26:66	Stony Coral Intraskeletal Polysaccharides	26:66	Structure and Function of Stony Coral Intraskeletal Polysaccharides.
30221225	2	14	from	evidences	483:491	arg1	process					537:543	the calcification process	519:543	the calcification process	519:543	This communication shows by a combination of techniques (nuclear magnetic resonance, Fourier transform infrared, and monosaccharide composition) that their key component is a 1→3 β-d glucuronic acid polymer and evidences its influence in vitro in the calcification process.
30221225	2	15	theme	monosaccharide	389:402	arg1	composition					404:414	monosaccharide composition	389:414	monosaccharide composition	389:414	This communication shows by a combination of techniques (nuclear magnetic resonance, Fourier transform infrared, and monosaccharide composition) that their key component is a 1→3 β-d glucuronic acid polymer and evidences its influence in vitro in the calcification process.
30221225	2	15	theme	monosaccharide	389:402	arg1	techniques					317:326	techniques	317:326	techniques (nuclear magnetic resonance, Fourier transform infrared, and monosaccharide composition)	317:415	This communication shows by a combination of techniques (nuclear magnetic resonance, Fourier transform infrared, and monosaccharide composition) that their key component is a 1→3 β-d glucuronic acid polymer and evidences its influence in vitro in the calcification process.
30221225	2	16	theme	1→3	447:449	arg1	component					432:440	their key component	422:440	their key component	422:440	This communication shows by a combination of techniques (nuclear magnetic resonance, Fourier transform infrared, and monosaccharide composition) that their key component is a 1→3 β-d glucuronic acid polymer and evidences its influence in vitro in the calcification process.
30221225	2	16	theme	1→3	447:449	arg1	polymer					471:477	a 1→3 β-d glucuronic acid polymer	445:477	a 1→3 β-d glucuronic acid polymer	445:477	This communication shows by a combination of techniques (nuclear magnetic resonance, Fourier transform infrared, and monosaccharide composition) that their key component is a 1→3 β-d glucuronic acid polymer and evidences its influence in vitro in the calcification process.
30221225	1	17	from	function	203:210	arg1	process					233:239	the calcification process	215:239	the calcification process	215:239	Polysaccharides represent a main weight fraction of the intraskeletal organic matrix of corals, but their structure, as well as their function in the calcification process, has been poorly investigated.
30221225	2	18	theme	nuclear	329:335	arg1	resonance					346:354	nuclear magnetic resonance	329:354	nuclear magnetic resonance	329:354	This communication shows by a combination of techniques (nuclear magnetic resonance, Fourier transform infrared, and monosaccharide composition) that their key component is a 1→3 β-d glucuronic acid polymer and evidences its influence in vitro in the calcification process.
30221225	2	18	theme	nuclear	329:335	arg1	techniques					317:326	techniques	317:326	techniques (nuclear magnetic resonance, Fourier transform infrared, and monosaccharide composition)	317:415	This communication shows by a combination of techniques (nuclear magnetic resonance, Fourier transform infrared, and monosaccharide composition) that their key component is a 1→3 β-d glucuronic acid polymer and evidences its influence in vitro in the calcification process.
30221225	0	19	theme	Intraskeletal	38:50	arg1	Polysaccharides					52:66	Stony Coral Intraskeletal Polysaccharides	26:66	Stony Coral Intraskeletal Polysaccharides	26:66	Structure and Function of Stony Coral Intraskeletal Polysaccharides.
30221225	1	20	theme	intraskeletal	125:137	arg1	matrix					147:152	the intraskeletal organic matrix	121:152	the intraskeletal organic matrix of corals	121:162	Polysaccharides represent a main weight fraction of the intraskeletal organic matrix of corals, but their structure, as well as their function in the calcification process, has been poorly investigated.
30221225	1	21	theme	organic	139:145	arg1	matrix					147:152	the intraskeletal organic matrix	121:152	the intraskeletal organic matrix of corals	121:162	Polysaccharides represent a main weight fraction of the intraskeletal organic matrix of corals, but their structure, as well as their function in the calcification process, has been poorly investigated.
30221225	2	22	dep	transform	365:373	arg1	infrared					375:382	infrared	375:382	transform infrared	365:382	This communication shows by a combination of techniques (nuclear magnetic resonance, Fourier transform infrared, and monosaccharide composition) that their key component is a 1→3 β-d glucuronic acid polymer and evidences its influence in vitro in the calcification process.
30221225	1	23	theme	matrix	147:152	arg1	fraction					109:116	a main weight fraction	95:116	a main weight fraction of the intraskeletal organic matrix of corals	95:162	Polysaccharides represent a main weight fraction of the intraskeletal organic matrix of corals, but their structure, as well as their function in the calcification process, has been poorly investigated.
30221225	2	24	theme	techniques	317:326	arg1	combination					302:312	a combination	300:312	a combination of techniques (nuclear magnetic resonance, Fourier transform infrared, and monosaccharide composition)	300:415	This communication shows by a combination of techniques (nuclear magnetic resonance, Fourier transform infrared, and monosaccharide composition) that their key component is a 1→3 β-d glucuronic acid polymer and evidences its influence in vitro in the calcification process.
30221225	1	25	theme	calcification	219:231	arg1	process					233:239	the calcification process	215:239	the calcification process	215:239	Polysaccharides represent a main weight fraction of the intraskeletal organic matrix of corals, but their structure, as well as their function in the calcification process, has been poorly investigated.
30221225	0	26	theme	Polysaccharides	52:66	arg1	Function					14:21	Function	14:21	Function	14:21	Structure and Function of Stony Coral Intraskeletal Polysaccharides.
30221225	0	26	theme	Polysaccharides	52:66	arg1	Structure					0:8	Structure	0:8	Structure	0:8	Structure and Function of Stony Coral Intraskeletal Polysaccharides.
30221225	1	27	theme	corals	157:162	arg1	matrix					147:152	the intraskeletal organic matrix	121:152	the intraskeletal organic matrix of corals	121:162	Polysaccharides represent a main weight fraction of the intraskeletal organic matrix of corals, but their structure, as well as their function in the calcification process, has been poorly investigated.
31195221	10	0	from	concentration	1512:1524	arg1	expression					1462:1471	the expression	1458:1471	colonic feces as well as the expression of G-protein coupled receptors	1433:1502	Furthermore, pectin apparently altered the p,p'-DDE-induced microbial composition and then promoted the levels of SCFAs in colonic feces as well as the expression of G-protein coupled receptors and the concentration of hormone peptide YY (PYY) and glucagon like peptide-1 (GLP-1).
31195221	10	0	from	concentration	1512:1524	arg1	feces					1441:1445	colonic feces	1433:1445	colonic feces as well as the expression of G-protein coupled receptors	1433:1502	Furthermore, pectin apparently altered the p,p'-DDE-induced microbial composition and then promoted the levels of SCFAs in colonic feces as well as the expression of G-protein coupled receptors and the concentration of hormone peptide YY (PYY) and glucagon like peptide-1 (GLP-1).
31195221	11	1	from	accumulation	1644:1655	arg1	tissues					1665:1671	mice tissues	1660:1671	mice tissues	1660:1671	Pectin treatment also significantly reduced p,p'-DDE accumulation in mice tissues during p,p'-DDE exposure but did not change p,p'-DDE metabolism after termination of p,p'-DDE exposure.
31195221	7	2	theme	signaling	1066:1074	arg1	pathway					1076:1082	the SCFAs-related signaling pathway	1048:1082	the SCFAs-related signaling pathway	1048:1082	Gut microbiota composition and the levels of short-chain fatty acids (SCFAs) as well as the receptor proteins and hormones in the SCFAs-related signaling pathway were analyzed.
31195221	4	3	dep	p'-dichlorodiphenyldichloroethylene	560:594	arg1	p					597:597	p	597:597	p	597:597	OBJECTIVES This study aimed to investigate the effects of pectin on obesity induced by a typical environmental pollutant p,p'-dichlorodiphenyldichloroethylene (p,p'-DDE) and explore the underlying mechanism by which pectin reversed p,p'-DDE-induced obesity.
31195221	4	3	dep	p'-dichlorodiphenyldichloroethylene	560:594	arg1	p'-DDE					599:604	p'-DDE	599:604	p'-DDE	599:604	OBJECTIVES This study aimed to investigate the effects of pectin on obesity induced by a typical environmental pollutant p,p'-dichlorodiphenyldichloroethylene (p,p'-DDE) and explore the underlying mechanism by which pectin reversed p,p'-DDE-induced obesity.
31195221	6	4	theme	plasma	855:860	arg1	profile					868:874	plasma lipid profile	855:874	plasma lipid profile	855:874	Body and fat weight gain, plasma lipid profile and insulin resistance of mice were assessed.
31195221	4	5	dep	OBJECTIVES	437:446	arg1	aimed					459:463	aimed	459:463	aimed to investigate the effects of pectin on obesity induced by a typical environmental pollutant p,p'-dichlorodiphenyldichloroethylene (p,p'-DDE) and explore the underlying mechanism by which pectin reversed p,p'-DDE-induced obesity	459:692	OBJECTIVES This study aimed to investigate the effects of pectin on obesity induced by a typical environmental pollutant p,p'-dichlorodiphenyldichloroethylene (p,p'-DDE) and explore the underlying mechanism by which pectin reversed p,p'-DDE-induced obesity.
31195221	12	6	theme	gut	1870:1872	arg1	microbiota					1874:1883	gut microbiota	1870:1883	gut microbiota	1870:1883	CONCLUSIONS Pectin had a good effect on reducing p,p'-DDE-induced obesity through regulating gut microbiota and provided a potential strategy for the treatment of environmental pollutant-caused health problems.
31195221	10	7	theme	coupled	1486:1492	arg1	receptors					1494:1502	G-protein coupled receptors	1476:1502	G-protein coupled receptors	1476:1502	Furthermore, pectin apparently altered the p,p'-DDE-induced microbial composition and then promoted the levels of SCFAs in colonic feces as well as the expression of G-protein coupled receptors and the concentration of hormone peptide YY (PYY) and glucagon like peptide-1 (GLP-1).
31195221	9	8	from	hyperglycemia	1247:1259	arg1	mice					1304:1307	p,p'-DDE-exposed mice	1287:1307	mice	1304:1307	RESULTS Pectin supplementation reversed body and fat weight gain, dyslipidemia, hyperglycemia and insulin resistance in p,p'-DDE-exposed mice.
31195221	6	9	theme	fat	838:840	arg1	gain					849:852	Body and fat weight gain	829:852	gain	849:852	Body and fat weight gain, plasma lipid profile and insulin resistance of mice were assessed.
31195221	10	10	theme	hormone	1529:1535	arg1	PYY					1549:1551	PYY	1549:1551	PYY	1549:1551	Furthermore, pectin apparently altered the p,p'-DDE-induced microbial composition and then promoted the levels of SCFAs in colonic feces as well as the expression of G-protein coupled receptors and the concentration of hormone peptide YY (PYY) and glucagon like peptide-1 (GLP-1).
31195221	10	10	theme	hormone	1529:1535	arg1	YY					1545:1546	hormone peptide YY	1529:1546	hormone peptide YY (PYY)	1529:1552	Furthermore, pectin apparently altered the p,p'-DDE-induced microbial composition and then promoted the levels of SCFAs in colonic feces as well as the expression of G-protein coupled receptors and the concentration of hormone peptide YY (PYY) and glucagon like peptide-1 (GLP-1).
31195221	7	11	theme	proteins	1023:1030	arg1	composition					937:947	Gut microbiota composition	922:947	Gut microbiota composition	922:947	Gut microbiota composition and the levels of short-chain fatty acids (SCFAs) as well as the receptor proteins and hormones in the SCFAs-related signaling pathway were analyzed.
31195221	7	11	theme	proteins	1023:1030	arg1	levels					957:962	the levels	953:962	the levels of short-chain fatty acids (SCFAs) as well as the receptor proteins and hormones in the SCFAs-related signaling pathway	953:1082	Gut microbiota composition and the levels of short-chain fatty acids (SCFAs) as well as the receptor proteins and hormones in the SCFAs-related signaling pathway were analyzed.
31195221	8	12	theme	various	1128:1134	arg1	tissues					1136:1142	various tissues	1128:1142	various tissues of mice	1128:1150	Moreover, p,p'-DDE levels in various tissues of mice were detected.
31195221	4	13	theme	underlying	623:632	arg1	mechanism					634:642	the underlying mechanism	619:642	the underlying mechanism by which pectin reversed p	619:669	OBJECTIVES This study aimed to investigate the effects of pectin on obesity induced by a typical environmental pollutant p,p'-dichlorodiphenyldichloroethylene (p,p'-DDE) and explore the underlying mechanism by which pectin reversed p,p'-DDE-induced obesity.
31195221	0	14	theme	p	114:114	arg1	study					105:109	A case study	98:109	gut microbiota: A case study of p,p'-DDE	82:121	Pectin reduces environmental pollutant-induced obesity in mice through regulating gut microbiota: A case study of p,p'-DDE.
31195221	5	15	theme	p	810:810	arg1	exposure					819:826	p,p'-DDE exposure	810:826	p,p'-DDE exposure	810:826	METHODS p,p'-DDE was used to induce obesity in C57BL/6J mice and pectin was supplied during and after cessation of p,p'-DDE exposure.
31195221	9	16	from	gain	1227:1230	arg1	mice					1304:1307	p,p'-DDE-exposed mice	1287:1307	mice	1304:1307	RESULTS Pectin supplementation reversed body and fat weight gain, dyslipidemia, hyperglycemia and insulin resistance in p,p'-DDE-exposed mice.
31195221	7	17	theme	short-chain	967:977	arg1	SCFAs					992:996	SCFAs	992:996	SCFAs	992:996	Gut microbiota composition and the levels of short-chain fatty acids (SCFAs) as well as the receptor proteins and hormones in the SCFAs-related signaling pathway were analyzed.
31195221	7	17	theme	short-chain	967:977	arg1	acids					985:989	short-chain fatty acids	967:989	short-chain fatty acids (SCFAs) as well as the receptor proteins and hormones	967:1043	Gut microbiota composition and the levels of short-chain fatty acids (SCFAs) as well as the receptor proteins and hormones in the SCFAs-related signaling pathway were analyzed.
31195221	12	18	theme	p'-DDE-induced	1828:1841	arg1	p					1826:1826	p	1826:1826	p	1826:1826	CONCLUSIONS Pectin had a good effect on reducing p,p'-DDE-induced obesity through regulating gut microbiota and provided a potential strategy for the treatment of environmental pollutant-caused health problems.
31195221	12	18	theme	p'-DDE-induced	1828:1841	arg1	obesity					1843:1849	p'-DDE-induced obesity	1828:1849	p'-DDE-induced obesity	1828:1849	CONCLUSIONS Pectin had a good effect on reducing p,p'-DDE-induced obesity through regulating gut microbiota and provided a potential strategy for the treatment of environmental pollutant-caused health problems.
31195221	3	19	theme	dietary	289:295	arg1	fiber					297:301	dietary fiber	289:301	dietary fiber	289:301	Many studies have demonstrated that dietary fiber could reduce obesity induced by high-fat diets, but whether environmental pollutant-induced obesity can be reversed is still unknown.
31195221	12	20	theme	problems	1978:1985	arg1	treatment					1927:1935	the treatment	1923:1935	the treatment of environmental pollutant-caused health problems	1923:1985	CONCLUSIONS Pectin had a good effect on reducing p,p'-DDE-induced obesity through regulating gut microbiota and provided a potential strategy for the treatment of environmental pollutant-caused health problems.
31195221	10	21	theme	YY	1545:1546	arg1	concentration					1512:1524	the concentration	1508:1524	the concentration of hormone peptide YY (PYY) and glucagon like peptide-1 (GLP-1)	1508:1588	Furthermore, pectin apparently altered the p,p'-DDE-induced microbial composition and then promoted the levels of SCFAs in colonic feces as well as the expression of G-protein coupled receptors and the concentration of hormone peptide YY (PYY) and glucagon like peptide-1 (GLP-1).
31195221	10	21	theme	YY	1545:1546	arg1	levels					1414:1419	the levels	1410:1419	the levels of SCFAs in colonic feces as well as the expression of G-protein coupled receptors	1410:1502	Furthermore, pectin apparently altered the p,p'-DDE-induced microbial composition and then promoted the levels of SCFAs in colonic feces as well as the expression of G-protein coupled receptors and the concentration of hormone peptide YY (PYY) and glucagon like peptide-1 (GLP-1).
31195221	8	22	from	levels	1118:1123	arg1	tissues					1136:1142	various tissues	1128:1142	various tissues of mice	1128:1150	Moreover, p,p'-DDE levels in various tissues of mice were detected.
31195221	6	23	theme	insulin	880:886	arg1	resistance					888:897	insulin resistance	880:897	insulin resistance	880:897	Body and fat weight gain, plasma lipid profile and insulin resistance of mice were assessed.
31195221	11	24	theme	mice	1660:1663	arg1	tissues					1665:1671	mice tissues	1660:1671	mice tissues	1660:1671	Pectin treatment also significantly reduced p,p'-DDE accumulation in mice tissues during p,p'-DDE exposure but did not change p,p'-DDE metabolism after termination of p,p'-DDE exposure.
31195221	9	25	theme	body	1207:1210	arg1	gain					1227:1230	body and fat weight gain	1207:1230	gain	1227:1230	RESULTS Pectin supplementation reversed body and fat weight gain, dyslipidemia, hyperglycemia and insulin resistance in p,p'-DDE-exposed mice.
31195221	4	26	theme	p'-DDE-induced	671:684	arg1	obesity					686:692	p'-DDE-induced obesity	671:692	p'-DDE-induced obesity	671:692	OBJECTIVES This study aimed to investigate the effects of pectin on obesity induced by a typical environmental pollutant p,p'-dichlorodiphenyldichloroethylene (p,p'-DDE) and explore the underlying mechanism by which pectin reversed p,p'-DDE-induced obesity.
31195221	9	27	theme	fat	1216:1218	arg1	gain					1227:1230	body and fat weight gain	1207:1230	gain	1227:1230	RESULTS Pectin supplementation reversed body and fat weight gain, dyslipidemia, hyperglycemia and insulin resistance in p,p'-DDE-exposed mice.
31195221	2	28	theme	main	229:232	arg1	causes					234:239	the main causes	225:239	the main causes of obesity	225:250	Environmental pollutants are one of the main causes of obesity.
31195221	7	29	theme	microbiota	926:935	arg1	composition					937:947	Gut microbiota composition	922:947	Gut microbiota composition	922:947	Gut microbiota composition and the levels of short-chain fatty acids (SCFAs) as well as the receptor proteins and hormones in the SCFAs-related signaling pathway were analyzed.
31195221	5	30	dep	METHODS	695:701	arg1	p					703:703	p	703:703	p	703:703	METHODS p,p'-DDE was used to induce obesity in C57BL/6J mice and pectin was supplied during and after cessation of p,p'-DDE exposure.
31195221	5	30	dep	METHODS	695:701	arg1	p'-DDE					705:710	p'-DDE	705:710	p'-DDE	705:710	METHODS p,p'-DDE was used to induce obesity in C57BL/6J mice and pectin was supplied during and after cessation of p,p'-DDE exposure.
31195221	12	31	theme	pollutant-caused	1954:1969	arg1	problems					1978:1985	environmental pollutant-caused health problems	1940:1985	environmental pollutant-caused health problems	1940:1985	CONCLUSIONS Pectin had a good effect on reducing p,p'-DDE-induced obesity through regulating gut microbiota and provided a potential strategy for the treatment of environmental pollutant-caused health problems.
31195221	10	32	theme	microbial	1370:1378	arg1	composition					1380:1390	p'-DDE-induced microbial composition	1355:1390	p'-DDE-induced microbial composition	1355:1390	Furthermore, pectin apparently altered the p,p'-DDE-induced microbial composition and then promoted the levels of SCFAs in colonic feces as well as the expression of G-protein coupled receptors and the concentration of hormone peptide YY (PYY) and glucagon like peptide-1 (GLP-1).
31195221	10	32	theme	microbial	1370:1378	arg1	p					1353:1353	the p	1349:1353	the p	1349:1353	Furthermore, pectin apparently altered the p,p'-DDE-induced microbial composition and then promoted the levels of SCFAs in colonic feces as well as the expression of G-protein coupled receptors and the concentration of hormone peptide YY (PYY) and glucagon like peptide-1 (GLP-1).
31195221	4	33	dep	pollutant	548:556	arg1	p					558:558	p	558:558	a typical environmental pollutant p	524:558	OBJECTIVES This study aimed to investigate the effects of pectin on obesity induced by a typical environmental pollutant p,p'-dichlorodiphenyldichloroethylene (p,p'-DDE) and explore the underlying mechanism by which pectin reversed p,p'-DDE-induced obesity.
31195221	3	34	theme	environmental	363:375	arg1	obesity					395:401	environmental pollutant-induced obesity	363:401	environmental pollutant-induced obesity	363:401	Many studies have demonstrated that dietary fiber could reduce obesity induced by high-fat diets, but whether environmental pollutant-induced obesity can be reversed is still unknown.
31195221	0	35	from	obesity	47:53	arg1	mice					58:61	mice	58:61	mice	58:61	Pectin reduces environmental pollutant-induced obesity in mice through regulating gut microbiota: A case study of p,p'-DDE.
31195221	10	36	theme	glucagon	1558:1565	arg1	concentration					1512:1524	the concentration	1508:1524	the concentration of hormone peptide YY (PYY) and glucagon like peptide-1 (GLP-1)	1508:1588	Furthermore, pectin apparently altered the p,p'-DDE-induced microbial composition and then promoted the levels of SCFAs in colonic feces as well as the expression of G-protein coupled receptors and the concentration of hormone peptide YY (PYY) and glucagon like peptide-1 (GLP-1).
31195221	10	36	theme	glucagon	1558:1565	arg1	levels					1414:1419	the levels	1410:1419	the levels of SCFAs in colonic feces as well as the expression of G-protein coupled receptors	1410:1502	Furthermore, pectin apparently altered the p,p'-DDE-induced microbial composition and then promoted the levels of SCFAs in colonic feces as well as the expression of G-protein coupled receptors and the concentration of hormone peptide YY (PYY) and glucagon like peptide-1 (GLP-1).
31195221	9	37	theme	insulin	1265:1271	arg1	resistance					1273:1282	insulin resistance	1265:1282	insulin resistance	1265:1282	RESULTS Pectin supplementation reversed body and fat weight gain, dyslipidemia, hyperglycemia and insulin resistance in p,p'-DDE-exposed mice.
31195221	9	38	from	resistance	1273:1282	arg1	mice					1304:1307	p,p'-DDE-exposed mice	1287:1307	mice	1304:1307	RESULTS Pectin supplementation reversed body and fat weight gain, dyslipidemia, hyperglycemia and insulin resistance in p,p'-DDE-exposed mice.
31195221	0	39	dep	microbiota	86:95	arg1	study					105:109	A case study	98:109	gut microbiota: A case study of p,p'-DDE	82:121	Pectin reduces environmental pollutant-induced obesity in mice through regulating gut microbiota: A case study of p,p'-DDE.
31195221	4	40	theme	environmental	534:546	arg1	pollutant					548:556	a typical environmental pollutant p	524:558	a typical environmental pollutant p	524:558	OBJECTIVES This study aimed to investigate the effects of pectin on obesity induced by a typical environmental pollutant p,p'-dichlorodiphenyldichloroethylene (p,p'-DDE) and explore the underlying mechanism by which pectin reversed p,p'-DDE-induced obesity.
31195221	4	40	theme	environmental	534:546	arg1	p'-dichlorodiphenyldichloroethylene					560:594	p'-dichlorodiphenyldichloroethylene	560:594	p'-dichlorodiphenyldichloroethylene (p,p'-DDE)	560:605	OBJECTIVES This study aimed to investigate the effects of pectin on obesity induced by a typical environmental pollutant p,p'-dichlorodiphenyldichloroethylene (p,p'-DDE) and explore the underlying mechanism by which pectin reversed p,p'-DDE-induced obesity.
31195221	10	41	theme	colonic	1433:1439	arg1	feces					1441:1445	colonic feces	1433:1445	colonic feces as well as the expression of G-protein coupled receptors	1433:1502	Furthermore, pectin apparently altered the p,p'-DDE-induced microbial composition and then promoted the levels of SCFAs in colonic feces as well as the expression of G-protein coupled receptors and the concentration of hormone peptide YY (PYY) and glucagon like peptide-1 (GLP-1).
31195221	1	42	dep	BACKGROUND	124:133	arg1	raised					165:170	raised	165:170	has raised global concerns	161:186	BACKGROUND The prevalence of obesity has raised global concerns.
31195221	0	43	theme	environmental	15:27	arg1	obesity					47:53	environmental pollutant-induced obesity	15:53	environmental pollutant-induced obesity in mice	15:61	Pectin reduces environmental pollutant-induced obesity in mice through regulating gut microbiota: A case study of p,p'-DDE.
31195221	10	44	theme	SCFAs	1424:1428	arg1	concentration					1512:1524	the concentration	1508:1524	the concentration of hormone peptide YY (PYY) and glucagon like peptide-1 (GLP-1)	1508:1588	Furthermore, pectin apparently altered the p,p'-DDE-induced microbial composition and then promoted the levels of SCFAs in colonic feces as well as the expression of G-protein coupled receptors and the concentration of hormone peptide YY (PYY) and glucagon like peptide-1 (GLP-1).
31195221	10	44	theme	SCFAs	1424:1428	arg1	levels					1414:1419	the levels	1410:1419	the levels of SCFAs in colonic feces as well as the expression of G-protein coupled receptors	1410:1502	Furthermore, pectin apparently altered the p,p'-DDE-induced microbial composition and then promoted the levels of SCFAs in colonic feces as well as the expression of G-protein coupled receptors and the concentration of hormone peptide YY (PYY) and glucagon like peptide-1 (GLP-1).
31195221	5	45	theme	exposure	819:826	arg1	cessation					797:805	cessation	797:805	cessation of p,p'-DDE exposure	797:826	METHODS p,p'-DDE was used to induce obesity in C57BL/6J mice and pectin was supplied during and after cessation of p,p'-DDE exposure.
31195221	2	46	theme	Environmental	189:201	arg1	pollutants					203:212	Environmental pollutants	189:212	Environmental pollutants	189:212	Environmental pollutants are one of the main causes of obesity.
31195221	10	47	theme	G-protein	1476:1484	arg1	receptors					1494:1502	G-protein coupled receptors	1476:1502	G-protein coupled receptors	1476:1502	Furthermore, pectin apparently altered the p,p'-DDE-induced microbial composition and then promoted the levels of SCFAs in colonic feces as well as the expression of G-protein coupled receptors and the concentration of hormone peptide YY (PYY) and glucagon like peptide-1 (GLP-1).
31195221	11	48	theme	p	1635:1635	arg1	accumulation					1644:1655	p,p'-DDE accumulation	1635:1655	p,p'-DDE accumulation in mice tissues	1635:1671	Pectin treatment also significantly reduced p,p'-DDE accumulation in mice tissues during p,p'-DDE exposure but did not change p,p'-DDE metabolism after termination of p,p'-DDE exposure.
31195221	12	49	theme	potential	1900:1908	arg1	strategy					1910:1917	a potential strategy	1898:1917	a potential strategy for the treatment of environmental pollutant-caused health problems	1898:1985	CONCLUSIONS Pectin had a good effect on reducing p,p'-DDE-induced obesity through regulating gut microbiota and provided a potential strategy for the treatment of environmental pollutant-caused health problems.
31195221	7	50	theme	SCFAs-related	1052:1064	arg1	pathway					1076:1082	the SCFAs-related signaling pathway	1048:1082	the SCFAs-related signaling pathway	1048:1082	Gut microbiota composition and the levels of short-chain fatty acids (SCFAs) as well as the receptor proteins and hormones in the SCFAs-related signaling pathway were analyzed.
31195221	11	51	theme	p	1758:1758	arg1	exposure					1767:1774	p,p'-DDE exposure	1758:1774	p,p'-DDE exposure	1758:1774	Pectin treatment also significantly reduced p,p'-DDE accumulation in mice tissues during p,p'-DDE exposure but did not change p,p'-DDE metabolism after termination of p,p'-DDE exposure.
31195221	6	52	theme	lipid	862:866	arg1	profile					868:874	plasma lipid profile	855:874	plasma lipid profile	855:874	Body and fat weight gain, plasma lipid profile and insulin resistance of mice were assessed.
31195221	10	53	theme	receptors	1494:1502	arg1	expression					1462:1471	the expression	1458:1471	colonic feces as well as the expression of G-protein coupled receptors	1433:1502	Furthermore, pectin apparently altered the p,p'-DDE-induced microbial composition and then promoted the levels of SCFAs in colonic feces as well as the expression of G-protein coupled receptors and the concentration of hormone peptide YY (PYY) and glucagon like peptide-1 (GLP-1).
31195221	10	53	theme	receptors	1494:1502	arg1	feces					1441:1445	colonic feces	1433:1445	colonic feces as well as the expression of G-protein coupled receptors	1433:1502	Furthermore, pectin apparently altered the p,p'-DDE-induced microbial composition and then promoted the levels of SCFAs in colonic feces as well as the expression of G-protein coupled receptors and the concentration of hormone peptide YY (PYY) and glucagon like peptide-1 (GLP-1).
31195221	4	54	from	effects	484:490	arg1	obesity					505:511	obesity	505:511	obesity induced by a typical environmental pollutant p,p'-dichlorodiphenyldichloroethylene (p,p'-DDE)	505:605	OBJECTIVES This study aimed to investigate the effects of pectin on obesity induced by a typical environmental pollutant p,p'-dichlorodiphenyldichloroethylene (p,p'-DDE) and explore the underlying mechanism by which pectin reversed p,p'-DDE-induced obesity.
31195221	1	55	theme	obesity	153:159	arg1	prevalence					139:148	The prevalence	135:148	The prevalence of obesity	135:159	BACKGROUND The prevalence of obesity has raised global concerns.
31195221	8	56	theme	p	1109:1109	arg1	levels					1118:1123	p,p'-DDE levels	1109:1123	p,p'-DDE levels in various tissues of mice	1109:1150	Moreover, p,p'-DDE levels in various tissues of mice were detected.
31195221	6	57	theme	weight	842:847	arg1	gain					849:852	Body and fat weight gain	829:852	gain	849:852	Body and fat weight gain, plasma lipid profile and insulin resistance of mice were assessed.
31195221	7	58	from	composition	937:947	arg1	pathway					1076:1082	the SCFAs-related signaling pathway	1048:1082	the SCFAs-related signaling pathway	1048:1082	Gut microbiota composition and the levels of short-chain fatty acids (SCFAs) as well as the receptor proteins and hormones in the SCFAs-related signaling pathway were analyzed.
31195221	10	59	theme	peptide	1537:1543	arg1	PYY					1549:1551	PYY	1549:1551	PYY	1549:1551	Furthermore, pectin apparently altered the p,p'-DDE-induced microbial composition and then promoted the levels of SCFAs in colonic feces as well as the expression of G-protein coupled receptors and the concentration of hormone peptide YY (PYY) and glucagon like peptide-1 (GLP-1).
31195221	10	59	theme	peptide	1537:1543	arg1	YY					1545:1546	hormone peptide YY	1529:1546	hormone peptide YY (PYY)	1529:1552	Furthermore, pectin apparently altered the p,p'-DDE-induced microbial composition and then promoted the levels of SCFAs in colonic feces as well as the expression of G-protein coupled receptors and the concentration of hormone peptide YY (PYY) and glucagon like peptide-1 (GLP-1).
31195221	7	60	theme	receptor	1014:1021	arg1	proteins					1023:1030	the receptor proteins	1010:1030	the receptor proteins	1010:1030	Gut microbiota composition and the levels of short-chain fatty acids (SCFAs) as well as the receptor proteins and hormones in the SCFAs-related signaling pathway were analyzed.
31195221	6	61	theme	mice	902:905	arg1	gain					849:852	Body and fat weight gain	829:852	gain	849:852	Body and fat weight gain, plasma lipid profile and insulin resistance of mice were assessed.
31195221	6	61	theme	mice	902:905	arg1	profile					868:874	plasma lipid profile	855:874	plasma lipid profile	855:874	Body and fat weight gain, plasma lipid profile and insulin resistance of mice were assessed.
31195221	6	61	theme	mice	902:905	arg1	resistance					888:897	insulin resistance	880:897	insulin resistance	880:897	Body and fat weight gain, plasma lipid profile and insulin resistance of mice were assessed.
31195221	3	62	theme	Many	253:256	arg1	studies					258:264	Many studies	253:264	Many studies	253:264	Many studies have demonstrated that dietary fiber could reduce obesity induced by high-fat diets, but whether environmental pollutant-induced obesity can be reversed is still unknown.
31195221	9	63	from	dyslipidemia	1233:1244	arg1	mice					1304:1307	p,p'-DDE-exposed mice	1287:1307	mice	1304:1307	RESULTS Pectin supplementation reversed body and fat weight gain, dyslipidemia, hyperglycemia and insulin resistance in p,p'-DDE-exposed mice.
31195221	9	64	theme	Pectin	1175:1180	arg1	supplementation					1182:1196	Pectin supplementation	1175:1196	Pectin supplementation	1175:1196	RESULTS Pectin supplementation reversed body and fat weight gain, dyslipidemia, hyperglycemia and insulin resistance in p,p'-DDE-exposed mice.
31195221	7	65	theme	fatty	979:983	arg1	SCFAs					992:996	SCFAs	992:996	SCFAs	992:996	Gut microbiota composition and the levels of short-chain fatty acids (SCFAs) as well as the receptor proteins and hormones in the SCFAs-related signaling pathway were analyzed.
31195221	7	65	theme	fatty	979:983	arg1	acids					985:989	short-chain fatty acids	967:989	short-chain fatty acids (SCFAs) as well as the receptor proteins and hormones	967:1043	Gut microbiota composition and the levels of short-chain fatty acids (SCFAs) as well as the receptor proteins and hormones in the SCFAs-related signaling pathway were analyzed.
31195221	8	66	theme	mice	1147:1150	arg1	tissues					1136:1142	various tissues	1128:1142	various tissues of mice	1128:1150	Moreover, p,p'-DDE levels in various tissues of mice were detected.
31195221	10	67	from	levels	1414:1419	arg1	expression					1462:1471	the expression	1458:1471	colonic feces as well as the expression of G-protein coupled receptors	1433:1502	Furthermore, pectin apparently altered the p,p'-DDE-induced microbial composition and then promoted the levels of SCFAs in colonic feces as well as the expression of G-protein coupled receptors and the concentration of hormone peptide YY (PYY) and glucagon like peptide-1 (GLP-1).
31195221	10	67	from	levels	1414:1419	arg1	feces					1441:1445	colonic feces	1433:1445	colonic feces as well as the expression of G-protein coupled receptors	1433:1502	Furthermore, pectin apparently altered the p,p'-DDE-induced microbial composition and then promoted the levels of SCFAs in colonic feces as well as the expression of G-protein coupled receptors and the concentration of hormone peptide YY (PYY) and glucagon like peptide-1 (GLP-1).
31195221	12	68	theme	health	1971:1976	arg1	problems					1978:1985	environmental pollutant-caused health problems	1940:1985	environmental pollutant-caused health problems	1940:1985	CONCLUSIONS Pectin had a good effect on reducing p,p'-DDE-induced obesity through regulating gut microbiota and provided a potential strategy for the treatment of environmental pollutant-caused health problems.
31195221	7	69	theme	acids	985:989	arg1	composition					937:947	Gut microbiota composition	922:947	Gut microbiota composition	922:947	Gut microbiota composition and the levels of short-chain fatty acids (SCFAs) as well as the receptor proteins and hormones in the SCFAs-related signaling pathway were analyzed.
31195221	7	69	theme	acids	985:989	arg1	levels					957:962	the levels	953:962	the levels of short-chain fatty acids (SCFAs) as well as the receptor proteins and hormones in the SCFAs-related signaling pathway	953:1082	Gut microbiota composition and the levels of short-chain fatty acids (SCFAs) as well as the receptor proteins and hormones in the SCFAs-related signaling pathway were analyzed.
31195221	2	70	theme	obesity	244:250	arg1	causes					234:239	the main causes	225:239	the main causes of obesity	225:250	Environmental pollutants are one of the main causes of obesity.
31195221	0	71	theme	gut	82:84	arg1	microbiota					86:95	gut microbiota	82:95	gut microbiota: A case study of p,p'-DDE	82:121	Pectin reduces environmental pollutant-induced obesity in mice through regulating gut microbiota: A case study of p,p'-DDE.
31195221	2	72	theme	causes	234:239	arg1	causes					234:239	the main causes	225:239	the main causes of obesity	225:250	Environmental pollutants are one of the main causes of obesity.
31195221	2	72	theme	causes	234:239	arg1	one					218:220	one	218:220	one	218:220	Environmental pollutants are one of the main causes of obesity.
31195221	7	73	from	levels	957:962	arg1	pathway					1076:1082	the SCFAs-related signaling pathway	1048:1082	the SCFAs-related signaling pathway	1048:1082	Gut microbiota composition and the levels of short-chain fatty acids (SCFAs) as well as the receptor proteins and hormones in the SCFAs-related signaling pathway were analyzed.
31195221	4	74	theme	pectin	495:500	arg1	effects					484:490	the effects	480:490	the effects of pectin on obesity induced by a typical environmental pollutant p,p'-dichlorodiphenyldichloroethylene (p,p'-DDE)	480:605	OBJECTIVES This study aimed to investigate the effects of pectin on obesity induced by a typical environmental pollutant p,p'-dichlorodiphenyldichloroethylene (p,p'-DDE) and explore the underlying mechanism by which pectin reversed p,p'-DDE-induced obesity.
31195221	5	75	theme	C57BL/6J	742:749	arg1	mice					751:754	C57BL/6J mice	742:754	C57BL/6J mice	742:754	METHODS p,p'-DDE was used to induce obesity in C57BL/6J mice and pectin was supplied during and after cessation of p,p'-DDE exposure.
31195221	9	76	theme	weight	1220:1225	arg1	gain					1227:1230	body and fat weight gain	1207:1230	gain	1227:1230	RESULTS Pectin supplementation reversed body and fat weight gain, dyslipidemia, hyperglycemia and insulin resistance in p,p'-DDE-exposed mice.
31195221	12	77	theme	good	1802:1805	arg1	effect					1807:1812	a good effect	1800:1812	a good effect	1800:1812	CONCLUSIONS Pectin had a good effect on reducing p,p'-DDE-induced obesity through regulating gut microbiota and provided a potential strategy for the treatment of environmental pollutant-caused health problems.
31195221	12	78	theme	environmental	1940:1952	arg1	problems					1978:1985	environmental pollutant-caused health problems	1940:1985	environmental pollutant-caused health problems	1940:1985	CONCLUSIONS Pectin had a good effect on reducing p,p'-DDE-induced obesity through regulating gut microbiota and provided a potential strategy for the treatment of environmental pollutant-caused health problems.
31195221	0	79	theme	case	100:103	arg1	study					105:109	A case study	98:109	gut microbiota: A case study of p,p'-DDE	82:121	Pectin reduces environmental pollutant-induced obesity in mice through regulating gut microbiota: A case study of p,p'-DDE.
31195221	5	80	used	used	716:719	arg2	METHODS					695:701	METHODS	695:701	METHODS p,p'-DDE	695:710	METHODS p,p'-DDE was used to induce obesity in C57BL/6J mice and pectin was supplied during and after cessation of p,p'-DDE exposure.
31195221	7	81	theme	Gut	922:924	arg1	composition					937:947	Gut microbiota composition	922:947	Gut microbiota composition	922:947	Gut microbiota composition and the levels of short-chain fatty acids (SCFAs) as well as the receptor proteins and hormones in the SCFAs-related signaling pathway were analyzed.
31195221	10	82	theme	p'-DDE-induced	1355:1368	arg1	composition					1380:1390	p'-DDE-induced microbial composition	1355:1390	p'-DDE-induced microbial composition	1355:1390	Furthermore, pectin apparently altered the p,p'-DDE-induced microbial composition and then promoted the levels of SCFAs in colonic feces as well as the expression of G-protein coupled receptors and the concentration of hormone peptide YY (PYY) and glucagon like peptide-1 (GLP-1).
31195221	10	82	theme	p'-DDE-induced	1355:1368	arg1	p					1353:1353	the p	1349:1353	the p	1349:1353	Furthermore, pectin apparently altered the p,p'-DDE-induced microbial composition and then promoted the levels of SCFAs in colonic feces as well as the expression of G-protein coupled receptors and the concentration of hormone peptide YY (PYY) and glucagon like peptide-1 (GLP-1).
31195221	11	83	theme	p	1680:1680	arg1	exposure					1689:1696	p,p'-DDE exposure	1680:1696	p,p'-DDE exposure	1680:1696	Pectin treatment also significantly reduced p,p'-DDE accumulation in mice tissues during p,p'-DDE exposure but did not change p,p'-DDE metabolism after termination of p,p'-DDE exposure.
31195221	12	84	theme	CONCLUSIONS	1777:1787	arg1	Pectin					1789:1794	CONCLUSIONS Pectin	1777:1794	CONCLUSIONS Pectin	1777:1794	CONCLUSIONS Pectin had a good effect on reducing p,p'-DDE-induced obesity through regulating gut microbiota and provided a potential strategy for the treatment of environmental pollutant-caused health problems.
31195221	3	85	theme	high-fat	335:342	arg1	diets					344:348	high-fat diets	335:348	high-fat diets	335:348	Many studies have demonstrated that dietary fiber could reduce obesity induced by high-fat diets, but whether environmental pollutant-induced obesity can be reversed is still unknown.
31195221	12	86	contain	had	1796:1798	arg1	Pectin					1789:1794	CONCLUSIONS Pectin	1777:1794	CONCLUSIONS Pectin	1777:1794	CONCLUSIONS Pectin had a good effect on reducing p,p'-DDE-induced obesity through regulating gut microbiota and provided a potential strategy for the treatment of environmental pollutant-caused health problems.
31195221	12	86	contain	had	1796:1798	arg2	effect					1807:1812	a good effect	1800:1812	a good effect	1800:1812	CONCLUSIONS Pectin had a good effect on reducing p,p'-DDE-induced obesity through regulating gut microbiota and provided a potential strategy for the treatment of environmental pollutant-caused health problems.
31195221	11	87	dep	p'-DDE	1719:1724	arg1	metabolism					1726:1735	metabolism	1726:1735	p'-DDE metabolism	1719:1735	Pectin treatment also significantly reduced p,p'-DDE accumulation in mice tissues during p,p'-DDE exposure but did not change p,p'-DDE metabolism after termination of p,p'-DDE exposure.
31195221	3	88	theme	pollutant-induced	377:393	arg1	obesity					395:401	environmental pollutant-induced obesity	363:401	environmental pollutant-induced obesity	363:401	Many studies have demonstrated that dietary fiber could reduce obesity induced by high-fat diets, but whether environmental pollutant-induced obesity can be reversed is still unknown.
31195221	0	89	theme	pollutant-induced	29:45	arg1	obesity					47:53	environmental pollutant-induced obesity	15:53	environmental pollutant-induced obesity in mice	15:61	Pectin reduces environmental pollutant-induced obesity in mice through regulating gut microbiota: A case study of p,p'-DDE.
31195221	9	90	theme	p	1287:1287	arg1	mice					1304:1307	p,p'-DDE-exposed mice	1287:1307	mice	1304:1307	RESULTS Pectin supplementation reversed body and fat weight gain, dyslipidemia, hyperglycemia and insulin resistance in p,p'-DDE-exposed mice.
31195221	6	91	theme	Body	829:832	arg1	gain					849:852	Body and fat weight gain	829:852	gain	849:852	Body and fat weight gain, plasma lipid profile and insulin resistance of mice were assessed.
31195221	11	92	theme	Pectin	1591:1596	arg1	treatment					1598:1606	Pectin treatment	1591:1606	Pectin treatment	1591:1606	Pectin treatment also significantly reduced p,p'-DDE accumulation in mice tissues during p,p'-DDE exposure but did not change p,p'-DDE metabolism after termination of p,p'-DDE exposure.
31195221	1	93	dep	global	172:177	arg1	concerns					179:186	concerns	179:186	concerns	179:186	BACKGROUND The prevalence of obesity has raised global concerns.
31195221	9	94	theme	p'-DDE-exposed	1289:1302	arg1	mice					1304:1307	p,p'-DDE-exposed mice	1287:1307	mice	1304:1307	RESULTS Pectin supplementation reversed body and fat weight gain, dyslipidemia, hyperglycemia and insulin resistance in p,p'-DDE-exposed mice.
31195221	4	95	theme	typical	526:532	arg1	pollutant					548:556	a typical environmental pollutant p	524:558	a typical environmental pollutant p	524:558	OBJECTIVES This study aimed to investigate the effects of pectin on obesity induced by a typical environmental pollutant p,p'-dichlorodiphenyldichloroethylene (p,p'-DDE) and explore the underlying mechanism by which pectin reversed p,p'-DDE-induced obesity.
31195221	4	95	theme	typical	526:532	arg1	p'-dichlorodiphenyldichloroethylene					560:594	p'-dichlorodiphenyldichloroethylene	560:594	p'-dichlorodiphenyldichloroethylene (p,p'-DDE)	560:605	OBJECTIVES This study aimed to investigate the effects of pectin on obesity induced by a typical environmental pollutant p,p'-dichlorodiphenyldichloroethylene (p,p'-DDE) and explore the underlying mechanism by which pectin reversed p,p'-DDE-induced obesity.
31195221	9	96	dep	RESULTS	1167:1173	arg1	reversed					1198:1205	reversed	1198:1205	reversed body and fat weight gain, dyslipidemia, hyperglycemia and insulin resistance in p,p'-DDE-exposed mice	1198:1307	RESULTS Pectin supplementation reversed body and fat weight gain, dyslipidemia, hyperglycemia and insulin resistance in p,p'-DDE-exposed mice.
31195221	11	97	theme	exposure	1767:1774	arg1	termination					1743:1753	termination	1743:1753	termination of p,p'-DDE exposure	1743:1774	Pectin treatment also significantly reduced p,p'-DDE accumulation in mice tissues during p,p'-DDE exposure but did not change p,p'-DDE metabolism after termination of p,p'-DDE exposure.
29803743	6	0	theme	films	840:844	arg1	properties					822:831	the mechanical properties	807:831	the mechanical properties of the films	807:844	The obtained results showed that γ-irradiation and the addition of tannic acid (TA) increased the mechanical properties of the films and the shelf-life of Rutab (Hyani) date during the marketing period (12 ± 2 °C, 98%, RH) from one week to one month of marketing period for consumers with accepted freshness and quality.
29803743	6	0	theme	films	840:844	arg1	shelf-life					854:863	the shelf-life	850:863	the shelf-life of Rutab (Hyani) date	850:885	The obtained results showed that γ-irradiation and the addition of tannic acid (TA) increased the mechanical properties of the films and the shelf-life of Rutab (Hyani) date during the marketing period (12 ± 2 °C, 98%, RH) from one week to one month of marketing period for consumers with accepted freshness and quality.
29803743	1	1	theme	coating	150:156	arg1	fruits					158:163	coating fruits	150:163	coating fruits film from natural biodegradable materials with evaluation of its efficiency in keeping the quality of fresh date fruits	150:283	The present work introduces a preparation of coating fruits film from natural biodegradable materials with evaluation of its efficiency in keeping the quality of fresh date fruits.
29803743	0	2	theme	fruit	72:76	arg1	behavior					78:85	fruit behavior	72:85	fruit behavior	72:85	Effect of edible co-polymers coatings using γ-irradiation on Hyani date fruit behavior during marketing.
29803743	1	3	theme	fruits	158:163	arg1	preparation					135:145	a preparation	133:145	a preparation of coating fruits film from natural biodegradable materials with evaluation of its efficiency in keeping the quality of fresh date fruits	133:283	The present work introduces a preparation of coating fruits film from natural biodegradable materials with evaluation of its efficiency in keeping the quality of fresh date fruits.
29803743	6	4	theme	marketing	898:906	arg1	period					908:913	the marketing period	894:913	the marketing period	894:913	The obtained results showed that γ-irradiation and the addition of tannic acid (TA) increased the mechanical properties of the films and the shelf-life of Rutab (Hyani) date during the marketing period (12 ± 2 °C, 98%, RH) from one week to one month of marketing period for consumers with accepted freshness and quality.
29803743	0	5	from	Effect	0:5	arg1	Hyani					61:65	Hyani	61:65	Hyani	61:65	Effect of edible co-polymers coatings using γ-irradiation on Hyani date fruit behavior during marketing.
29803743	2	6	theme	Triple	286:291	arg1	Tb					300:301	Tb	300:301	Tb	300:301	Triple blend (Tb) which involved PVA, chitosan (Cs) and tannic (TA) acids was studied in preservation of Rutab (Hyani) date.
29803743	2	6	theme	Triple	286:291	arg1	blend					293:297	Triple blend	286:297	Triple blend (Tb) which involved PVA, chitosan (Cs) and tannic (TA) acids	286:358	Triple blend (Tb) which involved PVA, chitosan (Cs) and tannic (TA) acids was studied in preservation of Rutab (Hyani) date.
29803743	6	7	dep	%	929:929	arg1	RH					932:933	RH	932:933	RH	932:933	The obtained results showed that γ-irradiation and the addition of tannic acid (TA) increased the mechanical properties of the films and the shelf-life of Rutab (Hyani) date during the marketing period (12 ± 2 °C, 98%, RH) from one week to one month of marketing period for consumers with accepted freshness and quality.
29803743	1	8	theme	fresh	267:271	arg1	fruits					278:283	fresh date fruits	267:283	fresh date fruits	267:283	The present work introduces a preparation of coating fruits film from natural biodegradable materials with evaluation of its efficiency in keeping the quality of fresh date fruits.
29803743	5	9	theme	mechanical	655:664	arg1	properties					688:697	the mechanical and thermo-mechanical properties	651:697	the mechanical and thermo-mechanical properties	651:697	The effects of polymer composition and irradiation dose on the mechanical and thermo-mechanical properties were studied.
29803743	6	10	theme	mechanical	811:820	arg1	properties					822:831	the mechanical properties	807:831	the mechanical properties of the films	807:844	The obtained results showed that γ-irradiation and the addition of tannic acid (TA) increased the mechanical properties of the films and the shelf-life of Rutab (Hyani) date during the marketing period (12 ± 2 °C, 98%, RH) from one week to one month of marketing period for consumers with accepted freshness and quality.
29803743	1	11	theme	natural	175:181	arg1	materials					197:205	natural biodegradable materials	175:205	natural biodegradable materials with evaluation of its efficiency in keeping the quality of fresh date fruits	175:283	The present work introduces a preparation of coating fruits film from natural biodegradable materials with evaluation of its efficiency in keeping the quality of fresh date fruits.
29803743	1	12	theme	film	165:168	arg1	fruits					158:163	coating fruits	150:163	coating fruits film from natural biodegradable materials with evaluation of its efficiency in keeping the quality of fresh date fruits	150:283	The present work introduces a preparation of coating fruits film from natural biodegradable materials with evaluation of its efficiency in keeping the quality of fresh date fruits.
29803743	3	13	theme	cold	469:472	arg1	storage					474:480	a cold storage	467:480	a cold storage	467:480	Antimicrobial characters besides decay of fruits during a cold storage were determined.
29803743	2	14	theme	Rutab	391:395	arg1	preservation					375:386	preservation	375:386	preservation of Rutab (Hyani) date	375:408	Triple blend (Tb) which involved PVA, chitosan (Cs) and tannic (TA) acids was studied in preservation of Rutab (Hyani) date.
29803743	2	15	dep	Rutab	391:395	arg1	date					405:408	date	405:408	Rutab (Hyani) date	391:408	Triple blend (Tb) which involved PVA, chitosan (Cs) and tannic (TA) acids was studied in preservation of Rutab (Hyani) date.
29803743	6	16	theme	obtained	717:724	arg1	results					726:732	The obtained results	713:732	The obtained results	713:732	The obtained results showed that γ-irradiation and the addition of tannic acid (TA) increased the mechanical properties of the films and the shelf-life of Rutab (Hyani) date during the marketing period (12 ± 2 °C, 98%, RH) from one week to one month of marketing period for consumers with accepted freshness and quality.
29803743	2	17	theme	tannic	342:347	arg1	acids					354:358	tannic (TA) acids	342:358	tannic (TA) acids	342:358	Triple blend (Tb) which involved PVA, chitosan (Cs) and tannic (TA) acids was studied in preservation of Rutab (Hyani) date.
29803743	5	18	theme	irradiation	631:641	arg1	dose					643:646	irradiation dose	631:646	irradiation dose	631:646	The effects of polymer composition and irradiation dose on the mechanical and thermo-mechanical properties were studied.
29803743	1	19	theme	date	273:276	arg1	fruits					278:283	fresh date fruits	267:283	fresh date fruits	267:283	The present work introduces a preparation of coating fruits film from natural biodegradable materials with evaluation of its efficiency in keeping the quality of fresh date fruits.
29803743	0	20	theme	co-polymers	17:27	arg1	coatings					29:36	edible co-polymers coatings	10:36	edible co-polymers coatings using γ-irradiation	10:56	Effect of edible co-polymers coatings using γ-irradiation on Hyani date fruit behavior during marketing.
29803743	1	21	theme	fruits	278:283	arg1	quality					256:262	the quality	252:262	the quality of fresh date fruits	252:283	The present work introduces a preparation of coating fruits film from natural biodegradable materials with evaluation of its efficiency in keeping the quality of fresh date fruits.
29803743	5	22	theme	composition	615:625	arg1	effects					596:602	The effects	592:602	The effects of polymer composition and irradiation dose on the mechanical and thermo-mechanical properties	592:697	The effects of polymer composition and irradiation dose on the mechanical and thermo-mechanical properties were studied.
29803743	0	23	theme	edible	10:15	arg1	coatings					29:36	edible co-polymers coatings	10:36	edible co-polymers coatings using γ-irradiation	10:56	Effect of edible co-polymers coatings using γ-irradiation on Hyani date fruit behavior during marketing.
29803743	4	24	theme	blend	503:507	arg1	solutions					509:517	The blend solutions	499:517	The blend solutions	499:517	The blend solutions were exposed to the γ-irradiation (5.0 to 20 kGy) before casting or use.
29803743	6	25	theme	accepted	1002:1009	arg1	freshness					1011:1019	accepted freshness	1002:1019	accepted freshness	1002:1019	The obtained results showed that γ-irradiation and the addition of tannic acid (TA) increased the mechanical properties of the films and the shelf-life of Rutab (Hyani) date during the marketing period (12 ± 2 °C, 98%, RH) from one week to one month of marketing period for consumers with accepted freshness and quality.
29803743	6	26	theme	marketing	966:974	arg1	period					976:981	marketing period	966:981	marketing period for consumers with accepted freshness and quality	966:1031	The obtained results showed that γ-irradiation and the addition of tannic acid (TA) increased the mechanical properties of the films and the shelf-life of Rutab (Hyani) date during the marketing period (12 ± 2 °C, 98%, RH) from one week to one month of marketing period for consumers with accepted freshness and quality.
29803743	1	27	with	materials	197:205	arg1	evaluation					212:221	evaluation	212:221	evaluation of its efficiency in keeping the quality of fresh date fruits	212:283	The present work introduces a preparation of coating fruits film from natural biodegradable materials with evaluation of its efficiency in keeping the quality of fresh date fruits.
29803743	1	28	dep	natural	175:181	arg1	biodegradable					183:195	biodegradable	183:195	biodegradable	183:195	The present work introduces a preparation of coating fruits film from natural biodegradable materials with evaluation of its efficiency in keeping the quality of fresh date fruits.
29803743	6	29	dep	increased	797:805	arg1	%					929:929	12 ± 2 °C, 98%	916:929	%	929:929	The obtained results showed that γ-irradiation and the addition of tannic acid (TA) increased the mechanical properties of the films and the shelf-life of Rutab (Hyani) date during the marketing period (12 ± 2 °C, 98%, RH) from one week to one month of marketing period for consumers with accepted freshness and quality.
29803743	0	30	theme	coatings	29:36	arg1	Effect					0:5	Effect	0:5	Effect of edible co-polymers coatings using γ-irradiation on Hyani	0:65	Effect of edible co-polymers coatings using γ-irradiation on Hyani date fruit behavior during marketing.
29803743	6	31	theme	Rutab	868:872	arg1	properties					822:831	the mechanical properties	807:831	the mechanical properties of the films	807:844	The obtained results showed that γ-irradiation and the addition of tannic acid (TA) increased the mechanical properties of the films and the shelf-life of Rutab (Hyani) date during the marketing period (12 ± 2 °C, 98%, RH) from one week to one month of marketing period for consumers with accepted freshness and quality.
29803743	6	31	theme	Rutab	868:872	arg1	shelf-life					854:863	the shelf-life	850:863	the shelf-life of Rutab (Hyani) date	850:885	The obtained results showed that γ-irradiation and the addition of tannic acid (TA) increased the mechanical properties of the films and the shelf-life of Rutab (Hyani) date during the marketing period (12 ± 2 °C, 98%, RH) from one week to one month of marketing period for consumers with accepted freshness and quality.
29803743	5	32	theme	dose	643:646	arg1	effects					596:602	The effects	592:602	The effects of polymer composition and irradiation dose on the mechanical and thermo-mechanical properties	592:697	The effects of polymer composition and irradiation dose on the mechanical and thermo-mechanical properties were studied.
29803743	6	33	dep	Rutab	868:872	arg1	date					882:885	date	882:885	Rutab (Hyani) date	868:885	The obtained results showed that γ-irradiation and the addition of tannic acid (TA) increased the mechanical properties of the films and the shelf-life of Rutab (Hyani) date during the marketing period (12 ± 2 °C, 98%, RH) from one week to one month of marketing period for consumers with accepted freshness and quality.
29803743	6	34	theme	acid	787:790	arg1	γ-irradiation					746:758	γ-irradiation	746:758	γ-irradiation	746:758	The obtained results showed that γ-irradiation and the addition of tannic acid (TA) increased the mechanical properties of the films and the shelf-life of Rutab (Hyani) date during the marketing period (12 ± 2 °C, 98%, RH) from one week to one month of marketing period for consumers with accepted freshness and quality.
29803743	6	34	theme	acid	787:790	arg1	addition					768:775	the addition	764:775	the addition of tannic acid (TA)	764:795	The obtained results showed that γ-irradiation and the addition of tannic acid (TA) increased the mechanical properties of the films and the shelf-life of Rutab (Hyani) date during the marketing period (12 ± 2 °C, 98%, RH) from one week to one month of marketing period for consumers with accepted freshness and quality.
29803743	4	35	dep	20 kGy	561:566	arg1	to					558:559	to	558:559	to	558:559	The blend solutions were exposed to the γ-irradiation (5.0 to 20 kGy) before casting or use.
29803743	1	36	theme	present	109:115	arg1	work					117:120	The present work	105:120	The present work	105:120	The present work introduces a preparation of coating fruits film from natural biodegradable materials with evaluation of its efficiency in keeping the quality of fresh date fruits.
29803743	6	37	with	consumers	987:995	arg1	quality					1025:1031	quality	1025:1031	quality	1025:1031	The obtained results showed that γ-irradiation and the addition of tannic acid (TA) increased the mechanical properties of the films and the shelf-life of Rutab (Hyani) date during the marketing period (12 ± 2 °C, 98%, RH) from one week to one month of marketing period for consumers with accepted freshness and quality.
29803743	6	37	with	consumers	987:995	arg1	freshness					1011:1019	accepted freshness	1002:1019	accepted freshness	1002:1019	The obtained results showed that γ-irradiation and the addition of tannic acid (TA) increased the mechanical properties of the films and the shelf-life of Rutab (Hyani) date during the marketing period (12 ± 2 °C, 98%, RH) from one week to one month of marketing period for consumers with accepted freshness and quality.
29803743	2	38	theme	TA	350:351	arg1	acids					354:358	tannic (TA) acids	342:358	tannic (TA) acids	342:358	Triple blend (Tb) which involved PVA, chitosan (Cs) and tannic (TA) acids was studied in preservation of Rutab (Hyani) date.
29803743	6	39	theme	tannic	780:785	arg1	TA					793:794	TA	793:794	TA	793:794	The obtained results showed that γ-irradiation and the addition of tannic acid (TA) increased the mechanical properties of the films and the shelf-life of Rutab (Hyani) date during the marketing period (12 ± 2 °C, 98%, RH) from one week to one month of marketing period for consumers with accepted freshness and quality.
29803743	6	39	theme	tannic	780:785	arg1	acid					787:790	tannic acid	780:790	tannic acid (TA)	780:795	The obtained results showed that γ-irradiation and the addition of tannic acid (TA) increased the mechanical properties of the films and the shelf-life of Rutab (Hyani) date during the marketing period (12 ± 2 °C, 98%, RH) from one week to one month of marketing period for consumers with accepted freshness and quality.
29803743	1	40	from	materials	197:205	arg1	film					165:168	film	165:168	film	165:168	The present work introduces a preparation of coating fruits film from natural biodegradable materials with evaluation of its efficiency in keeping the quality of fresh date fruits.
29803743	6	41	theme	period	976:981	arg1	month					957:961	one month	953:961	one month of marketing period for consumers with accepted freshness and quality	953:1031	The obtained results showed that γ-irradiation and the addition of tannic acid (TA) increased the mechanical properties of the films and the shelf-life of Rutab (Hyani) date during the marketing period (12 ± 2 °C, 98%, RH) from one week to one month of marketing period for consumers with accepted freshness and quality.
29803743	5	42	theme	polymer	607:613	arg1	composition					615:625	polymer composition	607:625	polymer composition	607:625	The effects of polymer composition and irradiation dose on the mechanical and thermo-mechanical properties were studied.
29803743	3	43	theme	Antimicrobial	411:423	arg1	characters					425:434	Antimicrobial characters	411:434	Antimicrobial characters besides decay of fruits during a cold storage	411:480	Antimicrobial characters besides decay of fruits during a cold storage were determined.
29803743	1	44	theme	efficiency	230:239	arg1	evaluation					212:221	evaluation	212:221	evaluation of its efficiency in keeping the quality of fresh date fruits	212:283	The present work introduces a preparation of coating fruits film from natural biodegradable materials with evaluation of its efficiency in keeping the quality of fresh date fruits.
29803743	5	45	theme	thermo-mechanical	670:686	arg1	properties					688:697	the mechanical and thermo-mechanical properties	651:697	the mechanical and thermo-mechanical properties	651:697	The effects of polymer composition and irradiation dose on the mechanical and thermo-mechanical properties were studied.
29803743	3	46	theme	fruits	453:458	arg1	decay					444:448	decay	444:448	decay of fruits during a cold storage	444:480	Antimicrobial characters besides decay of fruits during a cold storage were determined.
29803743	5	47	from	effects	596:602	arg1	properties					688:697	the mechanical and thermo-mechanical properties	651:697	the mechanical and thermo-mechanical properties	651:697	The effects of polymer composition and irradiation dose on the mechanical and thermo-mechanical properties were studied.
29853948	0	0	from	Optimization	11:22	arg1	Culture					120:126	Submerged Culture	110:126	Submerged Culture of Cordyceps gracilis (Grev	110:154	Retracted: Optimization, Composition, and Antioxidant Activities of Exo- and Intracellular Polysaccharides in Submerged Culture of Cordyceps gracilis (Grev.)
29853948	1	1	theme	Durieu	158:163	arg1	[This					173:177	Durieu & Mont. [This	158:177	Durieu & Mont. [This	158:177	Durieu & Mont. [This retracts the article DOI: 10.1155/2015/462864.]
29853948	0	2	theme	Exo-	68:71	arg1	Polysaccharides					91:105	Exo- and Intracellular Polysaccharides	68:105	Exo- and Intracellular Polysaccharides	68:105	Retracted: Optimization, Composition, and Antioxidant Activities of Exo- and Intracellular Polysaccharides in Submerged Culture of Cordyceps gracilis (Grev.)
29853948	1	3	theme	&	165:165	arg1	[This					173:177	Durieu & Mont. [This	158:177	Durieu & Mont. [This	158:177	Durieu & Mont. [This retracts the article DOI: 10.1155/2015/462864.]
29853948	0	4	from	Composition	25:35	arg1	Culture					120:126	Submerged Culture	110:126	Submerged Culture of Cordyceps gracilis (Grev	110:154	Retracted: Optimization, Composition, and Antioxidant Activities of Exo- and Intracellular Polysaccharides in Submerged Culture of Cordyceps gracilis (Grev.)
29853948	1	5	theme	article	192:198	arg1	DOI					200:202	the article DOI	188:202	the article DOI	188:202	Durieu & Mont. [This retracts the article DOI: 10.1155/2015/462864.]
29853948	0	6	theme	Polysaccharides	91:105	arg1	Optimization					11:22	Optimization	11:22	Optimization	11:22	Retracted: Optimization, Composition, and Antioxidant Activities of Exo- and Intracellular Polysaccharides in Submerged Culture of Cordyceps gracilis (Grev.)
29853948	0	6	theme	Polysaccharides	91:105	arg1	Activities					54:63	Antioxidant Activities	42:63	Antioxidant Activities	42:63	Retracted: Optimization, Composition, and Antioxidant Activities of Exo- and Intracellular Polysaccharides in Submerged Culture of Cordyceps gracilis (Grev.)
29853948	0	6	theme	Polysaccharides	91:105	arg1	Composition					25:35	Composition	25:35	Composition	25:35	Retracted: Optimization, Composition, and Antioxidant Activities of Exo- and Intracellular Polysaccharides in Submerged Culture of Cordyceps gracilis (Grev.)
29853948	1	7	theme	Mont.	167:171	arg1	[This					173:177	Durieu & Mont. [This	158:177	Durieu & Mont. [This	158:177	Durieu & Mont. [This retracts the article DOI: 10.1155/2015/462864.]
29853948	0	8	theme	Antioxidant	42:52	arg1	Activities					54:63	Antioxidant Activities	42:63	Antioxidant Activities	42:63	Retracted: Optimization, Composition, and Antioxidant Activities of Exo- and Intracellular Polysaccharides in Submerged Culture of Cordyceps gracilis (Grev.)
29853948	0	9	theme	Intracellular	77:89	arg1	Polysaccharides					91:105	Exo- and Intracellular Polysaccharides	68:105	Exo- and Intracellular Polysaccharides	68:105	Retracted: Optimization, Composition, and Antioxidant Activities of Exo- and Intracellular Polysaccharides in Submerged Culture of Cordyceps gracilis (Grev.)
29853948	0	10	theme	Grev	151:154	arg1	Culture					120:126	Submerged Culture	110:126	Submerged Culture of Cordyceps gracilis (Grev	110:154	Retracted: Optimization, Composition, and Antioxidant Activities of Exo- and Intracellular Polysaccharides in Submerged Culture of Cordyceps gracilis (Grev.)
29853948	0	11	from	Activities	54:63	arg1	Culture					120:126	Submerged Culture	110:126	Submerged Culture of Cordyceps gracilis (Grev	110:154	Retracted: Optimization, Composition, and Antioxidant Activities of Exo- and Intracellular Polysaccharides in Submerged Culture of Cordyceps gracilis (Grev.)
29853948	1	12	dep	retracts	179:186	arg1	10.1155/2015/462864					205:223	10.1155/2015/462864	205:223	10.1155/2015/462864	205:223	Durieu & Mont. [This retracts the article DOI: 10.1155/2015/462864.]
29853948	0	13	dep	Retracted	0:8	arg1	Optimization					11:22	Optimization	11:22	Optimization	11:22	Retracted: Optimization, Composition, and Antioxidant Activities of Exo- and Intracellular Polysaccharides in Submerged Culture of Cordyceps gracilis (Grev.)
29853948	0	13	dep	Retracted	0:8	arg1	Activities					54:63	Antioxidant Activities	42:63	Antioxidant Activities	42:63	Retracted: Optimization, Composition, and Antioxidant Activities of Exo- and Intracellular Polysaccharides in Submerged Culture of Cordyceps gracilis (Grev.)
29853948	0	13	dep	Retracted	0:8	arg1	Composition					25:35	Composition	25:35	Composition	25:35	Retracted: Optimization, Composition, and Antioxidant Activities of Exo- and Intracellular Polysaccharides in Submerged Culture of Cordyceps gracilis (Grev.)
29853948	0	14	theme	Submerged	110:118	arg1	Culture					120:126	Submerged Culture	110:126	Submerged Culture of Cordyceps gracilis (Grev	110:154	Retracted: Optimization, Composition, and Antioxidant Activities of Exo- and Intracellular Polysaccharides in Submerged Culture of Cordyceps gracilis (Grev.)
31668878	8	0	theme	Lactobacillus	897:909	arg1	14857T					929:934	Lactobacillus versmoldensis DSM 14857T	897:934	Lactobacillus versmoldensis DSM 14857T (97.9%)	897:942	The isolates are members of the Lactobacillus alimentarius group with Lactobacillus ginsenosidimutans DSM 24154T (99.4% 16S similarity), Lactobacillus versmoldensis DSM 14857T (97.9%) and Lactobacillus furfuricola DSM 27174T (97.7%) as phylogenetic closest related species and L. alimentarius DSM 20249T (97.7%) and Lactobacillus paralimentarius DSM 13961T (97.5%) as closest relatives, respectively.
31668878	8	0	theme	Lactobacillus	897:909	arg1	%					941:941	97.9%	937:941	97.9%	937:941	The isolates are members of the Lactobacillus alimentarius group with Lactobacillus ginsenosidimutans DSM 24154T (99.4% 16S similarity), Lactobacillus versmoldensis DSM 14857T (97.9%) and Lactobacillus furfuricola DSM 27174T (97.7%) as phylogenetic closest related species and L. alimentarius DSM 20249T (97.7%) and Lactobacillus paralimentarius DSM 13961T (97.5%) as closest relatives, respectively.
31668878	10	1	theme	A4α	1403:1405	arg1	type					1395:1398	the peptidoglycan type	1377:1398	the peptidoglycan type	1377:1398	For both designated type strains, the peptidoglycan type is A4α l-Lys-d-Asp and the major fatty acids are C16:0, C18:1ω9c and summed feature 7.
31668878	10	1	theme	A4α	1403:1405	arg1	l-Lys-d-Asp					1407:1417	A4α l-Lys-d-Asp	1403:1417	A4α l-Lys-d-Asp	1403:1417	For both designated type strains, the peptidoglycan type is A4α l-Lys-d-Asp and the major fatty acids are C16:0, C18:1ω9c and summed feature 7.
31668878	5	2	dep	isolates	338:345	arg1	1.1920					368:373	TMW 1.1920	364:373	TMW 1.1920	364:373	Two of the isolates (TMW 1.2172T and TMW 1.1920) are rod-shaped, whilst three isolates (TMW 1.2098T, TMW 1.2118 and TMW 1.2188) appear coccus shaped or as short rods.
31668878	5	2	dep	isolates	338:345	arg1	1.2172T					352:358	TMW 1.2172T	348:358	TMW 1.2172T	348:358	Two of the isolates (TMW 1.2172T and TMW 1.1920) are rod-shaped, whilst three isolates (TMW 1.2098T, TMW 1.2118 and TMW 1.2188) appear coccus shaped or as short rods.
31668878	8	3	with	group	819:823	arg1	24154T					866:871	Lactobacillus ginsenosidimutans DSM 24154T	830:871	Lactobacillus ginsenosidimutans DSM 24154T (99.4% 16S similarity)	830:894	The isolates are members of the Lactobacillus alimentarius group with Lactobacillus ginsenosidimutans DSM 24154T (99.4% 16S similarity), Lactobacillus versmoldensis DSM 14857T (97.9%) and Lactobacillus furfuricola DSM 27174T (97.7%) as phylogenetic closest related species and L. alimentarius DSM 20249T (97.7%) and Lactobacillus paralimentarius DSM 13961T (97.5%) as closest relatives, respectively.
31668878	8	3	with	group	819:823	arg1	27174T					978:983	Lactobacillus furfuricola DSM 27174T	948:983	Lactobacillus furfuricola DSM 27174T (97.7%)	948:991	The isolates are members of the Lactobacillus alimentarius group with Lactobacillus ginsenosidimutans DSM 24154T (99.4% 16S similarity), Lactobacillus versmoldensis DSM 14857T (97.9%) and Lactobacillus furfuricola DSM 27174T (97.7%) as phylogenetic closest related species and L. alimentarius DSM 20249T (97.7%) and Lactobacillus paralimentarius DSM 13961T (97.5%) as closest relatives, respectively.
31668878	8	3	with	group	819:823	arg1	%					941:941	97.9%	937:941	97.9%	937:941	The isolates are members of the Lactobacillus alimentarius group with Lactobacillus ginsenosidimutans DSM 24154T (99.4% 16S similarity), Lactobacillus versmoldensis DSM 14857T (97.9%) and Lactobacillus furfuricola DSM 27174T (97.7%) as phylogenetic closest related species and L. alimentarius DSM 20249T (97.7%) and Lactobacillus paralimentarius DSM 13961T (97.5%) as closest relatives, respectively.
31668878	8	3	with	group	819:823	arg1	%					990:990	97.7%	986:990	97.7%	986:990	The isolates are members of the Lactobacillus alimentarius group with Lactobacillus ginsenosidimutans DSM 24154T (99.4% 16S similarity), Lactobacillus versmoldensis DSM 14857T (97.9%) and Lactobacillus furfuricola DSM 27174T (97.7%) as phylogenetic closest related species and L. alimentarius DSM 20249T (97.7%) and Lactobacillus paralimentarius DSM 13961T (97.5%) as closest relatives, respectively.
31668878	8	3	with	group	819:823	arg1	similarity					884:893	99.4% 16S similarity	874:893	99.4% 16S similarity	874:893	The isolates are members of the Lactobacillus alimentarius group with Lactobacillus ginsenosidimutans DSM 24154T (99.4% 16S similarity), Lactobacillus versmoldensis DSM 14857T (97.9%) and Lactobacillus furfuricola DSM 27174T (97.7%) as phylogenetic closest related species and L. alimentarius DSM 20249T (97.7%) and Lactobacillus paralimentarius DSM 13961T (97.5%) as closest relatives, respectively.
31668878	8	3	with	group	819:823	arg1	14857T					929:934	Lactobacillus versmoldensis DSM 14857T	897:934	Lactobacillus versmoldensis DSM 14857T (97.9%)	897:942	The isolates are members of the Lactobacillus alimentarius group with Lactobacillus ginsenosidimutans DSM 24154T (99.4% 16S similarity), Lactobacillus versmoldensis DSM 14857T (97.9%) and Lactobacillus furfuricola DSM 27174T (97.7%) as phylogenetic closest related species and L. alimentarius DSM 20249T (97.7%) and Lactobacillus paralimentarius DSM 13961T (97.5%) as closest relatives, respectively.
31668878	8	4	theme	DSM	925:927	arg1	14857T					929:934	Lactobacillus versmoldensis DSM 14857T	897:934	Lactobacillus versmoldensis DSM 14857T (97.9%)	897:942	The isolates are members of the Lactobacillus alimentarius group with Lactobacillus ginsenosidimutans DSM 24154T (99.4% 16S similarity), Lactobacillus versmoldensis DSM 14857T (97.9%) and Lactobacillus furfuricola DSM 27174T (97.7%) as phylogenetic closest related species and L. alimentarius DSM 20249T (97.7%) and Lactobacillus paralimentarius DSM 13961T (97.5%) as closest relatives, respectively.
31668878	8	4	theme	DSM	925:927	arg1	%					941:941	97.9%	937:941	97.9%	937:941	The isolates are members of the Lactobacillus alimentarius group with Lactobacillus ginsenosidimutans DSM 24154T (99.4% 16S similarity), Lactobacillus versmoldensis DSM 14857T (97.9%) and Lactobacillus furfuricola DSM 27174T (97.7%) as phylogenetic closest related species and L. alimentarius DSM 20249T (97.7%) and Lactobacillus paralimentarius DSM 13961T (97.5%) as closest relatives, respectively.
31668878	11	5	theme	chemotaxonomic	1525:1538	arg1	analysis					1540:1547	phylogenetic, phenotypic and chemotaxonomic analysis	1496:1547	phylogenetic, phenotypic and chemotaxonomic analysis	1496:1547	Based on phylogenetic, phenotypic and chemotaxonomic analysis we demonstrated that the investigated isolates belong to two novel Lactobacillus species for which we propose the names Lactobacillus salsicarnum with the type strain TMW 1.2098T=DSM 109451T=LMG 31401Tand Lactobacillus halodurans with the type strain TMW 1.2172T=DSM 109452T=LMG 31402T.
31668878	7	6	theme	rpoA	672:675	arg1	sequences					682:690	rpoA gene sequences	672:690	rpoA gene sequences	672:690	Phylogenetic analysis of the 16S rRNA, dnaK, pheS and rpoA gene sequences revealed two distinct lineages within the genus Lactobacillus (L.).
31668878	10	7	theme	major	1427:1431	arg1	C18:1ω9c					1456:1463	C18:1ω9c	1456:1463	C18:1ω9c	1456:1463	For both designated type strains, the peptidoglycan type is A4α l-Lys-d-Asp and the major fatty acids are C16:0, C18:1ω9c and summed feature 7.
31668878	10	7	theme	major	1427:1431	arg1	acids					1439:1443	the major fatty acids	1423:1443	the major fatty acids	1423:1443	For both designated type strains, the peptidoglycan type is A4α l-Lys-d-Asp and the major fatty acids are C16:0, C18:1ω9c and summed feature 7.
31668878	11	8	theme	phenotypic	1510:1519	arg1	analysis					1540:1547	phylogenetic, phenotypic and chemotaxonomic analysis	1496:1547	phylogenetic, phenotypic and chemotaxonomic analysis	1496:1547	Based on phylogenetic, phenotypic and chemotaxonomic analysis we demonstrated that the investigated isolates belong to two novel Lactobacillus species for which we propose the names Lactobacillus salsicarnum with the type strain TMW 1.2098T=DSM 109451T=LMG 31401Tand Lactobacillus halodurans with the type strain TMW 1.2172T=DSM 109452T=LMG 31402T.
31668878	8	9	dep	Lactobacillus	830:842	arg1	ginsenosidimutans					844:860	ginsenosidimutans	844:860	ginsenosidimutans	844:860	The isolates are members of the Lactobacillus alimentarius group with Lactobacillus ginsenosidimutans DSM 24154T (99.4% 16S similarity), Lactobacillus versmoldensis DSM 14857T (97.9%) and Lactobacillus furfuricola DSM 27174T (97.7%) as phylogenetic closest related species and L. alimentarius DSM 20249T (97.7%) and Lactobacillus paralimentarius DSM 13961T (97.5%) as closest relatives, respectively.
31668878	7	10	theme	16S	647:649	arg1	rRNA					651:654	the 16S rRNA	643:654	the 16S rRNA	643:654	Phylogenetic analysis of the 16S rRNA, dnaK, pheS and rpoA gene sequences revealed two distinct lineages within the genus Lactobacillus (L.).
31668878	4	11	theme	blown	208:212	arg1	salami					227:232	blown pack spoiled salami	208:232	blown pack spoiled salami	208:232	Microbiota analysis of blown pack spoiled salami revealed five distinguishable Lactobacillus isolates we could not assign to a known species.
31668878	11	12	theme	type	1704:1707	arg1	31401Tand					1744:1752	the type strain TMW 1.2098T=DSM 109451T=LMG 31401Tand	1700:1752	the type strain TMW 1.2098T=DSM 109451T=LMG 31401Tand	1700:1752	Based on phylogenetic, phenotypic and chemotaxonomic analysis we demonstrated that the investigated isolates belong to two novel Lactobacillus species for which we propose the names Lactobacillus salsicarnum with the type strain TMW 1.2098T=DSM 109451T=LMG 31401Tand Lactobacillus halodurans with the type strain TMW 1.2172T=DSM 109452T=LMG 31402T.
31668878	8	13	theme	closest	1128:1134	arg1	relatives					1136:1144	closest relatives	1128:1144	closest relatives	1128:1144	The isolates are members of the Lactobacillus alimentarius group with Lactobacillus ginsenosidimutans DSM 24154T (99.4% 16S similarity), Lactobacillus versmoldensis DSM 14857T (97.9%) and Lactobacillus furfuricola DSM 27174T (97.7%) as phylogenetic closest related species and L. alimentarius DSM 20249T (97.7%) and Lactobacillus paralimentarius DSM 13961T (97.5%) as closest relatives, respectively.
31668878	10	14	theme	summed	1469:1474	arg1	feature					1476:1482	summed feature 7	1469:1484	summed feature 7	1469:1484	For both designated type strains, the peptidoglycan type is A4α l-Lys-d-Asp and the major fatty acids are C16:0, C18:1ω9c and summed feature 7.
31668878	9	15	theme	Average	1161:1167	arg1	ANI					1190:1192	ANI	1190:1192	ANI	1190:1192	Average Nucleotide Identity (ANI) and digital DNA-DNA hybridization (dDDH) values between the isolates and their close related type strains are lower than 80% and 25%, respectively.
31668878	9	15	theme	Average	1161:1167	arg1	Identity					1180:1187	Average Nucleotide Identity	1161:1187	Average Nucleotide Identity (ANI)	1161:1193	Average Nucleotide Identity (ANI) and digital DNA-DNA hybridization (dDDH) values between the isolates and their close related type strains are lower than 80% and 25%, respectively.
31668878	7	16	theme	rRNA	651:654	arg1	pheS					663:666	pheS	663:666	pheS	663:666	Phylogenetic analysis of the 16S rRNA, dnaK, pheS and rpoA gene sequences revealed two distinct lineages within the genus Lactobacillus (L.).
31668878	7	16	theme	rRNA	651:654	arg1	dnaK					657:660	dnaK	657:660	dnaK	657:660	Phylogenetic analysis of the 16S rRNA, dnaK, pheS and rpoA gene sequences revealed two distinct lineages within the genus Lactobacillus (L.).
31668878	7	16	theme	rRNA	651:654	arg1	sequences					682:690	rpoA gene sequences	672:690	rpoA gene sequences	672:690	Phylogenetic analysis of the 16S rRNA, dnaK, pheS and rpoA gene sequences revealed two distinct lineages within the genus Lactobacillus (L.).
31668878	7	16	theme	rRNA	651:654	arg1	analysis					631:638	Phylogenetic analysis	618:638	Phylogenetic analysis of the 16S rRNA	618:654	Phylogenetic analysis of the 16S rRNA, dnaK, pheS and rpoA gene sequences revealed two distinct lineages within the genus Lactobacillus (L.).
31668878	1	17	theme	halodurans	126:135	arg1	sp					137:138	Lactobacillus halodurans sp	112:138	Lactobacillus halodurans sp	112:138	isolated from blown salami packages: Description of Lactobacillus halodurans sp.
31668878	4	18	theme	distinguishable	248:262	arg1	isolates					278:285	five distinguishable Lactobacillus isolates	243:285	five distinguishable Lactobacillus isolates	243:285	Microbiota analysis of blown pack spoiled salami revealed five distinguishable Lactobacillus isolates we could not assign to a known species.
31668878	1	19	dep	isolated	60:67	arg1	Description					97:107	Description	97:107	Description of Lactobacillus halodurans sp	97:138	isolated from blown salami packages: Description of Lactobacillus halodurans sp.
31668878	8	20	dep	Lactobacillus	897:909	arg1	versmoldensis					911:923	versmoldensis	911:923	versmoldensis	911:923	The isolates are members of the Lactobacillus alimentarius group with Lactobacillus ginsenosidimutans DSM 24154T (99.4% 16S similarity), Lactobacillus versmoldensis DSM 14857T (97.9%) and Lactobacillus furfuricola DSM 27174T (97.7%) as phylogenetic closest related species and L. alimentarius DSM 20249T (97.7%) and Lactobacillus paralimentarius DSM 13961T (97.5%) as closest relatives, respectively.
31668878	11	21	theme	Lactobacillus	1616:1628	arg1	species					1630:1636	two novel Lactobacillus species	1606:1636	two novel Lactobacillus species for which we propose the names Lactobacillus salsicarnum with the type strain TMW 1.2098T=DSM 109451T=LMG 31401Tand Lactobacillus halodurans with the type strain TMW 1.2172T=DSM 109452T=LMG 31402T	1606:1833	Based on phylogenetic, phenotypic and chemotaxonomic analysis we demonstrated that the investigated isolates belong to two novel Lactobacillus species for which we propose the names Lactobacillus salsicarnum with the type strain TMW 1.2098T=DSM 109451T=LMG 31401Tand Lactobacillus halodurans with the type strain TMW 1.2172T=DSM 109452T=LMG 31402T.
31668878	9	22	theme	Identity	1180:1187	arg1	values					1236:1241	Average Nucleotide Identity (ANI) and digital DNA-DNA hybridization (dDDH) values	1161:1241	Average Nucleotide Identity (ANI) and digital DNA-DNA hybridization (dDDH) values between the isolates and their close related type strains	1161:1299	Average Nucleotide Identity (ANI) and digital DNA-DNA hybridization (dDDH) values between the isolates and their close related type strains are lower than 80% and 25%, respectively.
31668878	7	23	theme	Phylogenetic	618:629	arg1	analysis					631:638	Phylogenetic analysis	618:638	Phylogenetic analysis of the 16S rRNA	618:654	Phylogenetic analysis of the 16S rRNA, dnaK, pheS and rpoA gene sequences revealed two distinct lineages within the genus Lactobacillus (L.).
31668878	4	24	theme	spoiled	219:225	arg1	salami					227:232	blown pack spoiled salami	208:232	blown pack spoiled salami	208:232	Microbiota analysis of blown pack spoiled salami revealed five distinguishable Lactobacillus isolates we could not assign to a known species.
31668878	8	25	theme	Lactobacillus	792:804	arg1	group					819:823	the Lactobacillus alimentarius group	788:823	the Lactobacillus alimentarius group with Lactobacillus ginsenosidimutans DSM 24154T (99.4% 16S similarity), Lactobacillus versmoldensis DSM 14857T (97.9%) and Lactobacillus furfuricola DSM 27174T (97.7%)	788:991	The isolates are members of the Lactobacillus alimentarius group with Lactobacillus ginsenosidimutans DSM 24154T (99.4% 16S similarity), Lactobacillus versmoldensis DSM 14857T (97.9%) and Lactobacillus furfuricola DSM 27174T (97.7%) as phylogenetic closest related species and L. alimentarius DSM 20249T (97.7%) and Lactobacillus paralimentarius DSM 13961T (97.5%) as closest relatives, respectively.
31668878	5	26	theme	TMW	364:366	arg1	1.1920					368:373	TMW 1.1920	364:373	TMW 1.1920	364:373	Two of the isolates (TMW 1.2172T and TMW 1.1920) are rod-shaped, whilst three isolates (TMW 1.2098T, TMW 1.2118 and TMW 1.2188) appear coccus shaped or as short rods.
31668878	9	27	theme	DNA-DNA	1207:1213	arg1	hybridization					1215:1227	digital DNA-DNA hybridization	1199:1227	digital DNA-DNA hybridization (dDDH)	1199:1234	Average Nucleotide Identity (ANI) and digital DNA-DNA hybridization (dDDH) values between the isolates and their close related type strains are lower than 80% and 25%, respectively.
31668878	9	27	theme	DNA-DNA	1207:1213	arg1	dDDH					1230:1233	dDDH	1230:1233	dDDH	1230:1233	Average Nucleotide Identity (ANI) and digital DNA-DNA hybridization (dDDH) values between the isolates and their close related type strains are lower than 80% and 25%, respectively.
31668878	8	28	theme	group	819:823	arg1	isolates					764:771	The isolates	760:771	The isolates	760:771	The isolates are members of the Lactobacillus alimentarius group with Lactobacillus ginsenosidimutans DSM 24154T (99.4% 16S similarity), Lactobacillus versmoldensis DSM 14857T (97.9%) and Lactobacillus furfuricola DSM 27174T (97.7%) as phylogenetic closest related species and L. alimentarius DSM 20249T (97.7%) and Lactobacillus paralimentarius DSM 13961T (97.5%) as closest relatives, respectively.
31668878	8	28	theme	group	819:823	arg1	members					777:783	members	777:783	members of the Lactobacillus alimentarius group with Lactobacillus ginsenosidimutans DSM 24154T (99.4% 16S similarity), Lactobacillus versmoldensis DSM 14857T (97.9%) and Lactobacillus furfuricola DSM 27174T (97.7%)	777:991	The isolates are members of the Lactobacillus alimentarius group with Lactobacillus ginsenosidimutans DSM 24154T (99.4% 16S similarity), Lactobacillus versmoldensis DSM 14857T (97.9%) and Lactobacillus furfuricola DSM 27174T (97.7%) as phylogenetic closest related species and L. alimentarius DSM 20249T (97.7%) and Lactobacillus paralimentarius DSM 13961T (97.5%) as closest relatives, respectively.
31668878	11	29	theme	TMW	1800:1802	arg1	31402T					1828:1833	the type strain TMW 1.2172T=DSM 109452T=LMG 31402T	1784:1833	the type strain TMW 1.2172T=DSM 109452T=LMG 31402T	1784:1833	Based on phylogenetic, phenotypic and chemotaxonomic analysis we demonstrated that the investigated isolates belong to two novel Lactobacillus species for which we propose the names Lactobacillus salsicarnum with the type strain TMW 1.2098T=DSM 109451T=LMG 31401Tand Lactobacillus halodurans with the type strain TMW 1.2172T=DSM 109452T=LMG 31402T.
31668878	1	30	theme	salami	80:85	arg1	packages					87:94	blown salami packages	74:94	blown salami packages	74:94	isolated from blown salami packages: Description of Lactobacillus halodurans sp.
31668878	8	31	theme	Lactobacillus	830:842	arg1	similarity					884:893	99.4% 16S similarity	874:893	99.4% 16S similarity	874:893	The isolates are members of the Lactobacillus alimentarius group with Lactobacillus ginsenosidimutans DSM 24154T (99.4% 16S similarity), Lactobacillus versmoldensis DSM 14857T (97.9%) and Lactobacillus furfuricola DSM 27174T (97.7%) as phylogenetic closest related species and L. alimentarius DSM 20249T (97.7%) and Lactobacillus paralimentarius DSM 13961T (97.5%) as closest relatives, respectively.
31668878	8	31	theme	Lactobacillus	830:842	arg1	24154T					866:871	Lactobacillus ginsenosidimutans DSM 24154T	830:871	Lactobacillus ginsenosidimutans DSM 24154T (99.4% 16S similarity)	830:894	The isolates are members of the Lactobacillus alimentarius group with Lactobacillus ginsenosidimutans DSM 24154T (99.4% 16S similarity), Lactobacillus versmoldensis DSM 14857T (97.9%) and Lactobacillus furfuricola DSM 27174T (97.7%) as phylogenetic closest related species and L. alimentarius DSM 20249T (97.7%) and Lactobacillus paralimentarius DSM 13961T (97.5%) as closest relatives, respectively.
31668878	11	32	theme	type	1788:1791	arg1	31402T					1828:1833	the type strain TMW 1.2172T=DSM 109452T=LMG 31402T	1784:1833	the type strain TMW 1.2172T=DSM 109452T=LMG 31402T	1784:1833	Based on phylogenetic, phenotypic and chemotaxonomic analysis we demonstrated that the investigated isolates belong to two novel Lactobacillus species for which we propose the names Lactobacillus salsicarnum with the type strain TMW 1.2098T=DSM 109451T=LMG 31401Tand Lactobacillus halodurans with the type strain TMW 1.2172T=DSM 109452T=LMG 31402T.
31668878	0	33	theme	Polyphasic	0:9	arg1	characterization					11:26	Polyphasic characterization	0:26	Polyphasic characterization of two novel Lactobacillus spp.	0:58	Polyphasic characterization of two novel Lactobacillus spp.
31668878	8	34	theme	DSM	862:864	arg1	similarity					884:893	99.4% 16S similarity	874:893	99.4% 16S similarity	874:893	The isolates are members of the Lactobacillus alimentarius group with Lactobacillus ginsenosidimutans DSM 24154T (99.4% 16S similarity), Lactobacillus versmoldensis DSM 14857T (97.9%) and Lactobacillus furfuricola DSM 27174T (97.7%) as phylogenetic closest related species and L. alimentarius DSM 20249T (97.7%) and Lactobacillus paralimentarius DSM 13961T (97.5%) as closest relatives, respectively.
31668878	8	34	theme	DSM	862:864	arg1	24154T					866:871	Lactobacillus ginsenosidimutans DSM 24154T	830:871	Lactobacillus ginsenosidimutans DSM 24154T (99.4% 16S similarity)	830:894	The isolates are members of the Lactobacillus alimentarius group with Lactobacillus ginsenosidimutans DSM 24154T (99.4% 16S similarity), Lactobacillus versmoldensis DSM 14857T (97.9%) and Lactobacillus furfuricola DSM 27174T (97.7%) as phylogenetic closest related species and L. alimentarius DSM 20249T (97.7%) and Lactobacillus paralimentarius DSM 13961T (97.5%) as closest relatives, respectively.
31668878	5	35	dep	isolates	405:412	arg1	TMW					428:430	TMW	428:430	TMW	428:430	Two of the isolates (TMW 1.2172T and TMW 1.1920) are rod-shaped, whilst three isolates (TMW 1.2098T, TMW 1.2118 and TMW 1.2188) appear coccus shaped or as short rods.
31668878	5	35	dep	isolates	405:412	arg1	TMW					443:445	TMW	443:445	TMW	443:445	Two of the isolates (TMW 1.2172T and TMW 1.1920) are rod-shaped, whilst three isolates (TMW 1.2098T, TMW 1.2118 and TMW 1.2188) appear coccus shaped or as short rods.
31668878	5	35	dep	isolates	405:412	arg1	1.2098T					419:425	1.2098T	419:425	1.2098T	419:425	Two of the isolates (TMW 1.2172T and TMW 1.1920) are rod-shaped, whilst three isolates (TMW 1.2098T, TMW 1.2118 and TMW 1.2188) appear coccus shaped or as short rods.
31668878	5	35	dep	isolates	405:412	arg1	isolates					405:412	three isolates	399:412	three isolates (TMW 1.2098T, TMW 1.2118 and TMW 1.2188)	399:453	Two of the isolates (TMW 1.2172T and TMW 1.1920) are rod-shaped, whilst three isolates (TMW 1.2098T, TMW 1.2118 and TMW 1.2188) appear coccus shaped or as short rods.
31668878	8	36	theme	phylogenetic	996:1007	arg1	%					1069:1069	97.7%	1065:1069	97.7%	1065:1069	The isolates are members of the Lactobacillus alimentarius group with Lactobacillus ginsenosidimutans DSM 24154T (99.4% 16S similarity), Lactobacillus versmoldensis DSM 14857T (97.9%) and Lactobacillus furfuricola DSM 27174T (97.7%) as phylogenetic closest related species and L. alimentarius DSM 20249T (97.7%) and Lactobacillus paralimentarius DSM 13961T (97.5%) as closest relatives, respectively.
31668878	8	36	theme	phylogenetic	996:1007	arg1	species					1025:1031	phylogenetic closest related species	996:1031	phylogenetic closest related species	996:1031	The isolates are members of the Lactobacillus alimentarius group with Lactobacillus ginsenosidimutans DSM 24154T (99.4% 16S similarity), Lactobacillus versmoldensis DSM 14857T (97.9%) and Lactobacillus furfuricola DSM 27174T (97.7%) as phylogenetic closest related species and L. alimentarius DSM 20249T (97.7%) and Lactobacillus paralimentarius DSM 13961T (97.5%) as closest relatives, respectively.
31668878	10	37	theme	type	1363:1366	arg1	strains					1368:1374	both designated type strains	1347:1374	both designated type strains	1347:1374	For both designated type strains, the peptidoglycan type is A4α l-Lys-d-Asp and the major fatty acids are C16:0, C18:1ω9c and summed feature 7.
31668878	11	38	theme	109452T=LMG	1816:1826	arg1	31402T					1828:1833	the type strain TMW 1.2172T=DSM 109452T=LMG 31402T	1784:1833	the type strain TMW 1.2172T=DSM 109452T=LMG 31402T	1784:1833	Based on phylogenetic, phenotypic and chemotaxonomic analysis we demonstrated that the investigated isolates belong to two novel Lactobacillus species for which we propose the names Lactobacillus salsicarnum with the type strain TMW 1.2098T=DSM 109451T=LMG 31401Tand Lactobacillus halodurans with the type strain TMW 1.2172T=DSM 109452T=LMG 31402T.
31668878	0	39	theme	novel	35:39	arg1	spp					55:57	two novel Lactobacillus spp	31:57	two novel Lactobacillus spp	31:57	Polyphasic characterization of two novel Lactobacillus spp.
31668878	7	40	theme	genus	734:738	arg1	L					755:755	L.	755:756	L.	755:756	Phylogenetic analysis of the 16S rRNA, dnaK, pheS and rpoA gene sequences revealed two distinct lineages within the genus Lactobacillus (L.).
31668878	7	40	theme	genus	734:738	arg1	Lactobacillus					740:752	the genus Lactobacillus	730:752	the genus Lactobacillus (L.)	730:757	Phylogenetic analysis of the 16S rRNA, dnaK, pheS and rpoA gene sequences revealed two distinct lineages within the genus Lactobacillus (L.).
31668878	8	41	theme	related	1017:1023	arg1	%					1069:1069	97.7%	1065:1069	97.7%	1065:1069	The isolates are members of the Lactobacillus alimentarius group with Lactobacillus ginsenosidimutans DSM 24154T (99.4% 16S similarity), Lactobacillus versmoldensis DSM 14857T (97.9%) and Lactobacillus furfuricola DSM 27174T (97.7%) as phylogenetic closest related species and L. alimentarius DSM 20249T (97.7%) and Lactobacillus paralimentarius DSM 13961T (97.5%) as closest relatives, respectively.
31668878	8	41	theme	related	1017:1023	arg1	species					1025:1031	phylogenetic closest related species	996:1031	phylogenetic closest related species	996:1031	The isolates are members of the Lactobacillus alimentarius group with Lactobacillus ginsenosidimutans DSM 24154T (99.4% 16S similarity), Lactobacillus versmoldensis DSM 14857T (97.9%) and Lactobacillus furfuricola DSM 27174T (97.7%) as phylogenetic closest related species and L. alimentarius DSM 20249T (97.7%) and Lactobacillus paralimentarius DSM 13961T (97.5%) as closest relatives, respectively.
31668878	8	42	theme	%	878:878	arg1	similarity					884:893	99.4% 16S similarity	874:893	99.4% 16S similarity	874:893	The isolates are members of the Lactobacillus alimentarius group with Lactobacillus ginsenosidimutans DSM 24154T (99.4% 16S similarity), Lactobacillus versmoldensis DSM 14857T (97.9%) and Lactobacillus furfuricola DSM 27174T (97.7%) as phylogenetic closest related species and L. alimentarius DSM 20249T (97.7%) and Lactobacillus paralimentarius DSM 13961T (97.5%) as closest relatives, respectively.
31668878	8	42	theme	%	878:878	arg1	24154T					866:871	Lactobacillus ginsenosidimutans DSM 24154T	830:871	Lactobacillus ginsenosidimutans DSM 24154T (99.4% 16S similarity)	830:894	The isolates are members of the Lactobacillus alimentarius group with Lactobacillus ginsenosidimutans DSM 24154T (99.4% 16S similarity), Lactobacillus versmoldensis DSM 14857T (97.9%) and Lactobacillus furfuricola DSM 27174T (97.7%) as phylogenetic closest related species and L. alimentarius DSM 20249T (97.7%) and Lactobacillus paralimentarius DSM 13961T (97.5%) as closest relatives, respectively.
31668878	11	43	theme	109451T=LMG	1732:1742	arg1	31401Tand					1744:1752	the type strain TMW 1.2098T=DSM 109451T=LMG 31401Tand	1700:1752	the type strain TMW 1.2098T=DSM 109451T=LMG 31401Tand	1700:1752	Based on phylogenetic, phenotypic and chemotaxonomic analysis we demonstrated that the investigated isolates belong to two novel Lactobacillus species for which we propose the names Lactobacillus salsicarnum with the type strain TMW 1.2098T=DSM 109451T=LMG 31401Tand Lactobacillus halodurans with the type strain TMW 1.2172T=DSM 109452T=LMG 31402T.
31668878	0	44	theme	spp	55:57	arg1	characterization					11:26	Polyphasic characterization	0:26	Polyphasic characterization of two novel Lactobacillus spp.	0:58	Polyphasic characterization of two novel Lactobacillus spp.
31668878	10	45	theme	peptidoglycan	1381:1393	arg1	type					1395:1398	the peptidoglycan type	1377:1398	the peptidoglycan type	1377:1398	For both designated type strains, the peptidoglycan type is A4α l-Lys-d-Asp and the major fatty acids are C16:0, C18:1ω9c and summed feature 7.
31668878	10	45	theme	peptidoglycan	1381:1393	arg1	l-Lys-d-Asp					1407:1417	A4α l-Lys-d-Asp	1403:1417	A4α l-Lys-d-Asp	1403:1417	For both designated type strains, the peptidoglycan type is A4α l-Lys-d-Asp and the major fatty acids are C16:0, C18:1ω9c and summed feature 7.
31668878	11	46	theme	TMW	1716:1718	arg1	31401Tand					1744:1752	the type strain TMW 1.2098T=DSM 109451T=LMG 31401Tand	1700:1752	the type strain TMW 1.2098T=DSM 109451T=LMG 31401Tand	1700:1752	Based on phylogenetic, phenotypic and chemotaxonomic analysis we demonstrated that the investigated isolates belong to two novel Lactobacillus species for which we propose the names Lactobacillus salsicarnum with the type strain TMW 1.2098T=DSM 109451T=LMG 31401Tand Lactobacillus halodurans with the type strain TMW 1.2172T=DSM 109452T=LMG 31402T.
31668878	7	47	theme	distinct	705:712	arg1	lineages					714:721	two distinct lineages	701:721	two distinct lineages within the genus Lactobacillus (L.)	701:757	Phylogenetic analysis of the 16S rRNA, dnaK, pheS and rpoA gene sequences revealed two distinct lineages within the genus Lactobacillus (L.).
31668878	8	48	theme	alimentarius	1040:1051	arg1	20249T					1057:1062	L. alimentarius DSM 20249T	1037:1062	L. alimentarius DSM 20249T	1037:1062	The isolates are members of the Lactobacillus alimentarius group with Lactobacillus ginsenosidimutans DSM 24154T (99.4% 16S similarity), Lactobacillus versmoldensis DSM 14857T (97.9%) and Lactobacillus furfuricola DSM 27174T (97.7%) as phylogenetic closest related species and L. alimentarius DSM 20249T (97.7%) and Lactobacillus paralimentarius DSM 13961T (97.5%) as closest relatives, respectively.
31668878	7	49	theme	gene	677:680	arg1	sequences					682:690	rpoA gene sequences	672:690	rpoA gene sequences	672:690	Phylogenetic analysis of the 16S rRNA, dnaK, pheS and rpoA gene sequences revealed two distinct lineages within the genus Lactobacillus (L.).
31668878	2	50	dep	sp	176:177	arg1	nov.					141:144	nov.	141:144	nov.	141:144	nov. and Lactobacillus salsicarnum sp.
31668878	2	50	dep	sp	176:177	arg1	salsicarnum					164:174	Lactobacillus salsicarnum	150:174	Lactobacillus salsicarnum	150:174	nov. and Lactobacillus salsicarnum sp.
31668878	10	51	theme	fatty	1433:1437	arg1	C18:1ω9c					1456:1463	C18:1ω9c	1456:1463	C18:1ω9c	1456:1463	For both designated type strains, the peptidoglycan type is A4α l-Lys-d-Asp and the major fatty acids are C16:0, C18:1ω9c and summed feature 7.
31668878	10	51	theme	fatty	1433:1437	arg1	acids					1439:1443	the major fatty acids	1423:1443	the major fatty acids	1423:1443	For both designated type strains, the peptidoglycan type is A4α l-Lys-d-Asp and the major fatty acids are C16:0, C18:1ω9c and summed feature 7.
31668878	2	52	theme	Lactobacillus	150:162	arg1	salsicarnum					164:174	Lactobacillus salsicarnum	150:174	Lactobacillus salsicarnum	150:174	nov. and Lactobacillus salsicarnum sp.
31668878	8	53	theme	Lactobacillus	1076:1088	arg1	13961T					1110:1115	Lactobacillus paralimentarius DSM 13961T	1076:1115	Lactobacillus paralimentarius DSM 13961T (97.5%)	1076:1123	The isolates are members of the Lactobacillus alimentarius group with Lactobacillus ginsenosidimutans DSM 24154T (99.4% 16S similarity), Lactobacillus versmoldensis DSM 14857T (97.9%) and Lactobacillus furfuricola DSM 27174T (97.7%) as phylogenetic closest related species and L. alimentarius DSM 20249T (97.7%) and Lactobacillus paralimentarius DSM 13961T (97.5%) as closest relatives, respectively.
31668878	8	53	theme	Lactobacillus	1076:1088	arg1	%					1122:1122	97.5%	1118:1122	97.5%	1118:1122	The isolates are members of the Lactobacillus alimentarius group with Lactobacillus ginsenosidimutans DSM 24154T (99.4% 16S similarity), Lactobacillus versmoldensis DSM 14857T (97.9%) and Lactobacillus furfuricola DSM 27174T (97.7%) as phylogenetic closest related species and L. alimentarius DSM 20249T (97.7%) and Lactobacillus paralimentarius DSM 13961T (97.5%) as closest relatives, respectively.
31668878	8	54	theme	DSM	1106:1108	arg1	13961T					1110:1115	Lactobacillus paralimentarius DSM 13961T	1076:1115	Lactobacillus paralimentarius DSM 13961T (97.5%)	1076:1123	The isolates are members of the Lactobacillus alimentarius group with Lactobacillus ginsenosidimutans DSM 24154T (99.4% 16S similarity), Lactobacillus versmoldensis DSM 14857T (97.9%) and Lactobacillus furfuricola DSM 27174T (97.7%) as phylogenetic closest related species and L. alimentarius DSM 20249T (97.7%) and Lactobacillus paralimentarius DSM 13961T (97.5%) as closest relatives, respectively.
31668878	8	54	theme	DSM	1106:1108	arg1	%					1122:1122	97.5%	1118:1122	97.5%	1118:1122	The isolates are members of the Lactobacillus alimentarius group with Lactobacillus ginsenosidimutans DSM 24154T (99.4% 16S similarity), Lactobacillus versmoldensis DSM 14857T (97.9%) and Lactobacillus furfuricola DSM 27174T (97.7%) as phylogenetic closest related species and L. alimentarius DSM 20249T (97.7%) and Lactobacillus paralimentarius DSM 13961T (97.5%) as closest relatives, respectively.
31668878	4	55	theme	Microbiota	185:194	arg1	analysis					196:203	Microbiota analysis	185:203	Microbiota analysis of blown pack spoiled salami	185:232	Microbiota analysis of blown pack spoiled salami revealed five distinguishable Lactobacillus isolates we could not assign to a known species.
31668878	5	56	theme	isolates	338:345	arg1	Two					327:329	Two	327:329	Two	327:329	Two of the isolates (TMW 1.2172T and TMW 1.1920) are rod-shaped, whilst three isolates (TMW 1.2098T, TMW 1.2118 and TMW 1.2188) appear coccus shaped or as short rods.
31668878	5	56	theme	isolates	338:345	arg1	isolates					338:345	the isolates	334:345	the isolates (TMW 1.2172T and TMW 1.1920)	334:374	Two of the isolates (TMW 1.2172T and TMW 1.1920) are rod-shaped, whilst three isolates (TMW 1.2098T, TMW 1.2118 and TMW 1.2188) appear coccus shaped or as short rods.
31668878	8	57	theme	Lactobacillus	948:960	arg1	27174T					978:983	Lactobacillus furfuricola DSM 27174T	948:983	Lactobacillus furfuricola DSM 27174T (97.7%)	948:991	The isolates are members of the Lactobacillus alimentarius group with Lactobacillus ginsenosidimutans DSM 24154T (99.4% 16S similarity), Lactobacillus versmoldensis DSM 14857T (97.9%) and Lactobacillus furfuricola DSM 27174T (97.7%) as phylogenetic closest related species and L. alimentarius DSM 20249T (97.7%) and Lactobacillus paralimentarius DSM 13961T (97.5%) as closest relatives, respectively.
31668878	8	57	theme	Lactobacillus	948:960	arg1	%					990:990	97.7%	986:990	97.7%	986:990	The isolates are members of the Lactobacillus alimentarius group with Lactobacillus ginsenosidimutans DSM 24154T (99.4% 16S similarity), Lactobacillus versmoldensis DSM 14857T (97.9%) and Lactobacillus furfuricola DSM 27174T (97.7%) as phylogenetic closest related species and L. alimentarius DSM 20249T (97.7%) and Lactobacillus paralimentarius DSM 13961T (97.5%) as closest relatives, respectively.
31668878	5	58	theme	TMW	348:350	arg1	1.2172T					352:358	TMW 1.2172T	348:358	TMW 1.2172T	348:358	Two of the isolates (TMW 1.2172T and TMW 1.1920) are rod-shaped, whilst three isolates (TMW 1.2098T, TMW 1.2118 and TMW 1.2188) appear coccus shaped or as short rods.
31668878	11	59	theme	strain	1709:1714	arg1	31401Tand					1744:1752	the type strain TMW 1.2098T=DSM 109451T=LMG 31401Tand	1700:1752	the type strain TMW 1.2098T=DSM 109451T=LMG 31401Tand	1700:1752	Based on phylogenetic, phenotypic and chemotaxonomic analysis we demonstrated that the investigated isolates belong to two novel Lactobacillus species for which we propose the names Lactobacillus salsicarnum with the type strain TMW 1.2098T=DSM 109451T=LMG 31401Tand Lactobacillus halodurans with the type strain TMW 1.2172T=DSM 109452T=LMG 31402T.
31668878	1	60	theme	Lactobacillus	112:124	arg1	sp					137:138	Lactobacillus halodurans sp	112:138	Lactobacillus halodurans sp	112:138	isolated from blown salami packages: Description of Lactobacillus halodurans sp.
31668878	9	61	theme	Nucleotide	1169:1178	arg1	ANI					1190:1192	ANI	1190:1192	ANI	1190:1192	Average Nucleotide Identity (ANI) and digital DNA-DNA hybridization (dDDH) values between the isolates and their close related type strains are lower than 80% and 25%, respectively.
31668878	9	61	theme	Nucleotide	1169:1178	arg1	Identity					1180:1187	Average Nucleotide Identity	1161:1187	Average Nucleotide Identity (ANI)	1161:1193	Average Nucleotide Identity (ANI) and digital DNA-DNA hybridization (dDDH) values between the isolates and their close related type strains are lower than 80% and 25%, respectively.
31668878	1	62	theme	sp	137:138	arg1	Description					97:107	Description	97:107	Description of Lactobacillus halodurans sp	97:138	isolated from blown salami packages: Description of Lactobacillus halodurans sp.
31668878	5	63	theme	short	482:486	arg1	rods					488:491	short rods	482:491	short rods	482:491	Two of the isolates (TMW 1.2172T and TMW 1.1920) are rod-shaped, whilst three isolates (TMW 1.2098T, TMW 1.2118 and TMW 1.2188) appear coccus shaped or as short rods.
31668878	4	64	theme	Lactobacillus	264:276	arg1	isolates					278:285	five distinguishable Lactobacillus isolates	243:285	five distinguishable Lactobacillus isolates	243:285	Microbiota analysis of blown pack spoiled salami revealed five distinguishable Lactobacillus isolates we could not assign to a known species.
31668878	9	65	theme	related	1280:1286	arg1	strains					1293:1299	their close related type strains	1268:1299	their close related type strains	1268:1299	Average Nucleotide Identity (ANI) and digital DNA-DNA hybridization (dDDH) values between the isolates and their close related type strains are lower than 80% and 25%, respectively.
31668878	11	66	theme	novel	1610:1614	arg1	species					1630:1636	two novel Lactobacillus species	1606:1636	two novel Lactobacillus species for which we propose the names Lactobacillus salsicarnum with the type strain TMW 1.2098T=DSM 109451T=LMG 31401Tand Lactobacillus halodurans with the type strain TMW 1.2172T=DSM 109452T=LMG 31402T	1606:1833	Based on phylogenetic, phenotypic and chemotaxonomic analysis we demonstrated that the investigated isolates belong to two novel Lactobacillus species for which we propose the names Lactobacillus salsicarnum with the type strain TMW 1.2098T=DSM 109451T=LMG 31401Tand Lactobacillus halodurans with the type strain TMW 1.2172T=DSM 109452T=LMG 31402T.
31668878	8	67	theme	DSM	974:976	arg1	27174T					978:983	Lactobacillus furfuricola DSM 27174T	948:983	Lactobacillus furfuricola DSM 27174T (97.7%)	948:991	The isolates are members of the Lactobacillus alimentarius group with Lactobacillus ginsenosidimutans DSM 24154T (99.4% 16S similarity), Lactobacillus versmoldensis DSM 14857T (97.9%) and Lactobacillus furfuricola DSM 27174T (97.7%) as phylogenetic closest related species and L. alimentarius DSM 20249T (97.7%) and Lactobacillus paralimentarius DSM 13961T (97.5%) as closest relatives, respectively.
31668878	8	67	theme	DSM	974:976	arg1	%					990:990	97.7%	986:990	97.7%	986:990	The isolates are members of the Lactobacillus alimentarius group with Lactobacillus ginsenosidimutans DSM 24154T (99.4% 16S similarity), Lactobacillus versmoldensis DSM 14857T (97.9%) and Lactobacillus furfuricola DSM 27174T (97.7%) as phylogenetic closest related species and L. alimentarius DSM 20249T (97.7%) and Lactobacillus paralimentarius DSM 13961T (97.5%) as closest relatives, respectively.
31668878	4	68	theme	pack	214:217	arg1	salami					227:232	blown pack spoiled salami	208:232	blown pack spoiled salami	208:232	Microbiota analysis of blown pack spoiled salami revealed five distinguishable Lactobacillus isolates we could not assign to a known species.
31668878	8	69	dep	Lactobacillus	1076:1088	arg1	paralimentarius					1090:1104	paralimentarius	1090:1104	paralimentarius	1090:1104	The isolates are members of the Lactobacillus alimentarius group with Lactobacillus ginsenosidimutans DSM 24154T (99.4% 16S similarity), Lactobacillus versmoldensis DSM 14857T (97.9%) and Lactobacillus furfuricola DSM 27174T (97.7%) as phylogenetic closest related species and L. alimentarius DSM 20249T (97.7%) and Lactobacillus paralimentarius DSM 13961T (97.5%) as closest relatives, respectively.
31668878	9	70	theme	digital	1199:1205	arg1	hybridization					1215:1227	digital DNA-DNA hybridization	1199:1227	digital DNA-DNA hybridization (dDDH)	1199:1234	Average Nucleotide Identity (ANI) and digital DNA-DNA hybridization (dDDH) values between the isolates and their close related type strains are lower than 80% and 25%, respectively.
31668878	9	70	theme	digital	1199:1205	arg1	dDDH					1230:1233	dDDH	1230:1233	dDDH	1230:1233	Average Nucleotide Identity (ANI) and digital DNA-DNA hybridization (dDDH) values between the isolates and their close related type strains are lower than 80% and 25%, respectively.
31668878	4	71	theme	salami	227:232	arg1	analysis					196:203	Microbiota analysis	185:203	Microbiota analysis of blown pack spoiled salami	185:232	Microbiota analysis of blown pack spoiled salami revealed five distinguishable Lactobacillus isolates we could not assign to a known species.
31668878	8	72	dep	Lactobacillus	948:960	arg1	furfuricola					962:972	furfuricola	962:972	furfuricola	962:972	The isolates are members of the Lactobacillus alimentarius group with Lactobacillus ginsenosidimutans DSM 24154T (99.4% 16S similarity), Lactobacillus versmoldensis DSM 14857T (97.9%) and Lactobacillus furfuricola DSM 27174T (97.7%) as phylogenetic closest related species and L. alimentarius DSM 20249T (97.7%) and Lactobacillus paralimentarius DSM 13961T (97.5%) as closest relatives, respectively.
31668878	9	73	theme	hybridization	1215:1227	arg1	values					1236:1241	Average Nucleotide Identity (ANI) and digital DNA-DNA hybridization (dDDH) values	1161:1241	Average Nucleotide Identity (ANI) and digital DNA-DNA hybridization (dDDH) values between the isolates and their close related type strains	1161:1299	Average Nucleotide Identity (ANI) and digital DNA-DNA hybridization (dDDH) values between the isolates and their close related type strains are lower than 80% and 25%, respectively.
31668878	1	74	theme	blown	74:78	arg1	packages					87:94	blown salami packages	74:94	blown salami packages	74:94	isolated from blown salami packages: Description of Lactobacillus halodurans sp.
31668878	11	75	theme	1.2172T=DSM	1804:1814	arg1	31402T					1828:1833	the type strain TMW 1.2172T=DSM 109452T=LMG 31402T	1784:1833	the type strain TMW 1.2172T=DSM 109452T=LMG 31402T	1784:1833	Based on phylogenetic, phenotypic and chemotaxonomic analysis we demonstrated that the investigated isolates belong to two novel Lactobacillus species for which we propose the names Lactobacillus salsicarnum with the type strain TMW 1.2098T=DSM 109451T=LMG 31401Tand Lactobacillus halodurans with the type strain TMW 1.2172T=DSM 109452T=LMG 31402T.
31668878	4	76	theme	known	312:316	arg1	species					318:324	a known species	310:324	a known species	310:324	Microbiota analysis of blown pack spoiled salami revealed five distinguishable Lactobacillus isolates we could not assign to a known species.
31668878	11	77	theme	strain	1793:1798	arg1	31402T					1828:1833	the type strain TMW 1.2172T=DSM 109452T=LMG 31402T	1784:1833	the type strain TMW 1.2172T=DSM 109452T=LMG 31402T	1784:1833	Based on phylogenetic, phenotypic and chemotaxonomic analysis we demonstrated that the investigated isolates belong to two novel Lactobacillus species for which we propose the names Lactobacillus salsicarnum with the type strain TMW 1.2098T=DSM 109451T=LMG 31401Tand Lactobacillus halodurans with the type strain TMW 1.2172T=DSM 109452T=LMG 31402T.
31668878	8	78	theme	closest	1009:1015	arg1	%					1069:1069	97.7%	1065:1069	97.7%	1065:1069	The isolates are members of the Lactobacillus alimentarius group with Lactobacillus ginsenosidimutans DSM 24154T (99.4% 16S similarity), Lactobacillus versmoldensis DSM 14857T (97.9%) and Lactobacillus furfuricola DSM 27174T (97.7%) as phylogenetic closest related species and L. alimentarius DSM 20249T (97.7%) and Lactobacillus paralimentarius DSM 13961T (97.5%) as closest relatives, respectively.
31668878	8	78	theme	closest	1009:1015	arg1	species					1025:1031	phylogenetic closest related species	996:1031	phylogenetic closest related species	996:1031	The isolates are members of the Lactobacillus alimentarius group with Lactobacillus ginsenosidimutans DSM 24154T (99.4% 16S similarity), Lactobacillus versmoldensis DSM 14857T (97.9%) and Lactobacillus furfuricola DSM 27174T (97.7%) as phylogenetic closest related species and L. alimentarius DSM 20249T (97.7%) and Lactobacillus paralimentarius DSM 13961T (97.5%) as closest relatives, respectively.
31668878	0	79	theme	Lactobacillus	41:53	arg1	spp					55:57	two novel Lactobacillus spp	31:57	two novel Lactobacillus spp	31:57	Polyphasic characterization of two novel Lactobacillus spp.
31668878	10	80	theme	designated	1352:1361	arg1	strains					1368:1374	both designated type strains	1347:1374	both designated type strains	1347:1374	For both designated type strains, the peptidoglycan type is A4α l-Lys-d-Asp and the major fatty acids are C16:0, C18:1ω9c and summed feature 7.
31668878	8	81	dep	Lactobacillus	792:804	arg1	alimentarius					806:817	alimentarius	806:817	alimentarius	806:817	The isolates are members of the Lactobacillus alimentarius group with Lactobacillus ginsenosidimutans DSM 24154T (99.4% 16S similarity), Lactobacillus versmoldensis DSM 14857T (97.9%) and Lactobacillus furfuricola DSM 27174T (97.7%) as phylogenetic closest related species and L. alimentarius DSM 20249T (97.7%) and Lactobacillus paralimentarius DSM 13961T (97.5%) as closest relatives, respectively.
31668878	8	82	theme	L.	1037:1038	arg1	20249T					1057:1062	L. alimentarius DSM 20249T	1037:1062	L. alimentarius DSM 20249T	1037:1062	The isolates are members of the Lactobacillus alimentarius group with Lactobacillus ginsenosidimutans DSM 24154T (99.4% 16S similarity), Lactobacillus versmoldensis DSM 14857T (97.9%) and Lactobacillus furfuricola DSM 27174T (97.7%) as phylogenetic closest related species and L. alimentarius DSM 20249T (97.7%) and Lactobacillus paralimentarius DSM 13961T (97.5%) as closest relatives, respectively.
31668878	9	83	theme	close	1274:1278	arg1	strains					1293:1299	their close related type strains	1268:1299	their close related type strains	1268:1299	Average Nucleotide Identity (ANI) and digital DNA-DNA hybridization (dDDH) values between the isolates and their close related type strains are lower than 80% and 25%, respectively.
31668878	8	84	theme	DSM	1053:1055	arg1	20249T					1057:1062	L. alimentarius DSM 20249T	1037:1062	L. alimentarius DSM 20249T	1037:1062	The isolates are members of the Lactobacillus alimentarius group with Lactobacillus ginsenosidimutans DSM 24154T (99.4% 16S similarity), Lactobacillus versmoldensis DSM 14857T (97.9%) and Lactobacillus furfuricola DSM 27174T (97.7%) as phylogenetic closest related species and L. alimentarius DSM 20249T (97.7%) and Lactobacillus paralimentarius DSM 13961T (97.5%) as closest relatives, respectively.
31668878	8	85	theme	16S	880:882	arg1	similarity					884:893	99.4% 16S similarity	874:893	99.4% 16S similarity	874:893	The isolates are members of the Lactobacillus alimentarius group with Lactobacillus ginsenosidimutans DSM 24154T (99.4% 16S similarity), Lactobacillus versmoldensis DSM 14857T (97.9%) and Lactobacillus furfuricola DSM 27174T (97.7%) as phylogenetic closest related species and L. alimentarius DSM 20249T (97.7%) and Lactobacillus paralimentarius DSM 13961T (97.5%) as closest relatives, respectively.
31668878	8	85	theme	16S	880:882	arg1	24154T					866:871	Lactobacillus ginsenosidimutans DSM 24154T	830:871	Lactobacillus ginsenosidimutans DSM 24154T (99.4% 16S similarity)	830:894	The isolates are members of the Lactobacillus alimentarius group with Lactobacillus ginsenosidimutans DSM 24154T (99.4% 16S similarity), Lactobacillus versmoldensis DSM 14857T (97.9%) and Lactobacillus furfuricola DSM 27174T (97.7%) as phylogenetic closest related species and L. alimentarius DSM 20249T (97.7%) and Lactobacillus paralimentarius DSM 13961T (97.5%) as closest relatives, respectively.
31668878	11	86	theme	investigated	1574:1585	arg1	isolates					1587:1594	the investigated isolates	1570:1594	the investigated isolates	1570:1594	Based on phylogenetic, phenotypic and chemotaxonomic analysis we demonstrated that the investigated isolates belong to two novel Lactobacillus species for which we propose the names Lactobacillus salsicarnum with the type strain TMW 1.2098T=DSM 109451T=LMG 31401Tand Lactobacillus halodurans with the type strain TMW 1.2172T=DSM 109452T=LMG 31402T.
31668878	11	87	theme	1.2098T=DSM	1720:1730	arg1	31401Tand					1744:1752	the type strain TMW 1.2098T=DSM 109451T=LMG 31401Tand	1700:1752	the type strain TMW 1.2098T=DSM 109451T=LMG 31401Tand	1700:1752	Based on phylogenetic, phenotypic and chemotaxonomic analysis we demonstrated that the investigated isolates belong to two novel Lactobacillus species for which we propose the names Lactobacillus salsicarnum with the type strain TMW 1.2098T=DSM 109451T=LMG 31401Tand Lactobacillus halodurans with the type strain TMW 1.2172T=DSM 109452T=LMG 31402T.
31668878	9	88	theme	type	1288:1291	arg1	strains					1293:1299	their close related type strains	1268:1299	their close related type strains	1268:1299	Average Nucleotide Identity (ANI) and digital DNA-DNA hybridization (dDDH) values between the isolates and their close related type strains are lower than 80% and 25%, respectively.
31668878	11	89	theme	phylogenetic	1496:1507	arg1	analysis					1540:1547	phylogenetic, phenotypic and chemotaxonomic analysis	1496:1547	phylogenetic, phenotypic and chemotaxonomic analysis	1496:1547	Based on phylogenetic, phenotypic and chemotaxonomic analysis we demonstrated that the investigated isolates belong to two novel Lactobacillus species for which we propose the names Lactobacillus salsicarnum with the type strain TMW 1.2098T=DSM 109451T=LMG 31401Tand Lactobacillus halodurans with the type strain TMW 1.2172T=DSM 109452T=LMG 31402T.
30667229	0	0	theme	Matrix	96:101	arg1	Niche					103:107	Their Extracellular Matrix Niche	76:107	Their Extracellular Matrix Niche	76:107	Identification and Characterization of Canine Ligament Progenitor Cells and Their Extracellular Matrix Niche.
30667229	5	1	theme	technique	786:794	arg1	application					728:738	the novel application	718:738	the novel application of a metabolic labeling and mass spectrometry technique	718:794	The extracellular matrix (ECM) composition was characterized by the novel application of a metabolic labeling and mass spectrometry technique.
30667229	5	2	theme	matrix	672:677	arg1	composition					685:695	The extracellular matrix (ECM) composition	654:695	The extracellular matrix (ECM) composition	654:695	The extracellular matrix (ECM) composition was characterized by the novel application of a metabolic labeling and mass spectrometry technique.
30667229	8	3	theme	turnover	1042:1049	arg1	profiles					1051:1058	unique turnover profiles	1035:1058	unique turnover profiles for distinct ECM protein groups	1035:1090	Metabolic labeling of cells demonstrated unique turnover profiles for distinct ECM protein groups, indicating the importance of certain niche components for LPC survival and function.
30667229	9	4	theme	ligament	1348:1355	arg1	strategies					1364:1373	potential ligament repair strategies	1338:1373	potential ligament repair strategies	1338:1373	The newly synthesized niche components identified in this study could be exploited to aid identification of LPCs and to promote their survival and function for potential ligament repair strategies.
30667229	1	5	theme	treatment	242:250	arg1	options					252:258	poor and current treatment options	225:258	poor and current treatment options ineffective	225:270	Ligaments are prone to injury and degeneration in humans and animals, however the healing potential of ligament is poor and current treatment options ineffective.
30667229	1	5	theme	treatment	242:250	arg1	potential					200:208	the healing potential	188:208	the healing potential of ligament	188:220	Ligaments are prone to injury and degeneration in humans and animals, however the healing potential of ligament is poor and current treatment options ineffective.
30667229	0	6	theme	Extracellular	82:94	arg1	Niche					103:107	Their Extracellular Matrix Niche	76:107	Their Extracellular Matrix Niche	76:107	Identification and Characterization of Canine Ligament Progenitor Cells and Their Extracellular Matrix Niche.
30667229	8	7	theme	distinct	1064:1071	arg1	groups					1085:1090	distinct ECM protein groups	1064:1090	distinct ECM protein groups	1064:1090	Metabolic labeling of cells demonstrated unique turnover profiles for distinct ECM protein groups, indicating the importance of certain niche components for LPC survival and function.
30667229	6	8	theme	stem	848:851	arg1	expression					865:874	stem cell marker expression	848:874	stem cell marker expression	848:874	LPCs demonstrated clonogenicity, multipotency, and stem cell marker expression.
30667229	7	9	theme	collagens	899:907	arg1	number					879:884	A number	877:884	A number of different collagens, glycoproteins, and proteoglycans	877:941	A number of different collagens, glycoproteins, and proteoglycans were identified in the LPC niche using proteomics.
30667229	8	10	theme	ECM	1073:1075	arg1	groups					1085:1090	distinct ECM protein groups	1064:1090	distinct ECM protein groups	1064:1090	Metabolic labeling of cells demonstrated unique turnover profiles for distinct ECM protein groups, indicating the importance of certain niche components for LPC survival and function.
30667229	9	11	theme	potential	1338:1346	arg1	strategies					1364:1373	potential ligament repair strategies	1338:1373	potential ligament repair strategies	1338:1373	The newly synthesized niche components identified in this study could be exploited to aid identification of LPCs and to promote their survival and function for potential ligament repair strategies.
30667229	1	12	theme	ineffective	260:270	arg1	options					252:258	poor and current treatment options	225:258	poor and current treatment options ineffective	225:270	Ligaments are prone to injury and degeneration in humans and animals, however the healing potential of ligament is poor and current treatment options ineffective.
30667229	1	12	theme	ineffective	260:270	arg1	potential					200:208	the healing potential	188:208	the healing potential of ligament	188:220	Ligaments are prone to injury and degeneration in humans and animals, however the healing potential of ligament is poor and current treatment options ineffective.
30667229	0	13	theme	Niche	103:107	arg1	Characterization					19:34	Characterization	19:34	Characterization	19:34	Identification and Characterization of Canine Ligament Progenitor Cells and Their Extracellular Matrix Niche.
30667229	0	13	theme	Niche	103:107	arg1	Identification					0:13	Identification	0:13	Identification	0:13	Identification and Characterization of Canine Ligament Progenitor Cells and Their Extracellular Matrix Niche.
30667229	5	14	theme	novel	722:726	arg1	application					728:738	the novel application	718:738	the novel application of a metabolic labeling and mass spectrometry technique	718:794	The extracellular matrix (ECM) composition was characterized by the novel application of a metabolic labeling and mass spectrometry technique.
30667229	8	15	theme	protein	1077:1083	arg1	groups					1085:1090	distinct ECM protein groups	1064:1090	distinct ECM protein groups	1064:1090	Metabolic labeling of cells demonstrated unique turnover profiles for distinct ECM protein groups, indicating the importance of certain niche components for LPC survival and function.
30667229	1	16	from	degeneration	144:155	arg1	animals					171:177	animals	171:177	animals	171:177	Ligaments are prone to injury and degeneration in humans and animals, however the healing potential of ligament is poor and current treatment options ineffective.
30667229	1	16	from	degeneration	144:155	arg1	humans					160:165	humans	160:165	humans	160:165	Ligaments are prone to injury and degeneration in humans and animals, however the healing potential of ligament is poor and current treatment options ineffective.
30667229	5	17	theme	labeling	755:762	arg1	technique					786:794	a metabolic labeling and mass spectrometry technique	743:794	a metabolic labeling and mass spectrometry technique	743:794	The extracellular matrix (ECM) composition was characterized by the novel application of a metabolic labeling and mass spectrometry technique.
30667229	9	18	theme	repair	1357:1362	arg1	strategies					1364:1373	potential ligament repair strategies	1338:1373	potential ligament repair strategies	1338:1373	The newly synthesized niche components identified in this study could be exploited to aid identification of LPCs and to promote their survival and function for potential ligament repair strategies.
30667229	8	19	theme	cells	1016:1020	arg1	labeling					1004:1011	Metabolic labeling	994:1011	Metabolic labeling of cells	994:1020	Metabolic labeling of cells demonstrated unique turnover profiles for distinct ECM protein groups, indicating the importance of certain niche components for LPC survival and function.
30667229	2	20	theme	cell-based	278:287	arg1	therapies					289:297	Stem cell-based therapies	273:297	Stem cell-based therapies	273:297	Stem cell-based therapies hold potential for treatment of ligament injuries.
30667229	7	21	theme	different	889:897	arg1	collagens					899:907	different collagens	889:907	different collagens	889:907	A number of different collagens, glycoproteins, and proteoglycans were identified in the LPC niche using proteomics.
30667229	0	22	theme	Progenitor	55:64	arg1	Cells					66:70	Canine Ligament Progenitor Cells	39:70	Canine Ligament Progenitor Cells	39:70	Identification and Characterization of Canine Ligament Progenitor Cells and Their Extracellular Matrix Niche.
30667229	1	23	from	injury	133:138	arg1	animals					171:177	animals	171:177	animals	171:177	Ligaments are prone to injury and degeneration in humans and animals, however the healing potential of ligament is poor and current treatment options ineffective.
30667229	1	23	from	injury	133:138	arg1	humans					160:165	humans	160:165	humans	160:165	Ligaments are prone to injury and degeneration in humans and animals, however the healing potential of ligament is poor and current treatment options ineffective.
30667229	2	24	theme	Stem	273:276	arg1	therapies					289:297	Stem cell-based therapies	273:297	Stem cell-based therapies	273:297	Stem cell-based therapies hold potential for treatment of ligament injuries.
30667229	3	25	theme	specific	443:450	arg1	components					458:467	specific niche components	443:467	specific niche components which could promote the survival and function of LPCs	443:521	This study aimed to characterize a ligament progenitor cell (LPC) population and to identify specific niche components which could promote the survival and function of LPCs.
30667229	3	26	theme	progenitor	394:403	arg1	population					416:425	a ligament progenitor cell (LPC) population	383:425	a ligament progenitor cell (LPC) population	383:425	This study aimed to characterize a ligament progenitor cell (LPC) population and to identify specific niche components which could promote the survival and function of LPCs.
30667229	8	27	theme	unique	1035:1040	arg1	profiles					1051:1058	unique turnover profiles	1035:1058	unique turnover profiles for distinct ECM protein groups	1035:1090	Metabolic labeling of cells demonstrated unique turnover profiles for distinct ECM protein groups, indicating the importance of certain niche components for LPC survival and function.
30667229	3	28	theme	cell	405:408	arg1	population					416:425	a ligament progenitor cell (LPC) population	383:425	a ligament progenitor cell (LPC) population	383:425	This study aimed to characterize a ligament progenitor cell (LPC) population and to identify specific niche components which could promote the survival and function of LPCs.
30667229	4	29	theme	cranial	555:561	arg1	ligament					572:579	canine cranial cruciate ligament	548:579	canine cranial cruciate ligament	548:579	LPCs were isolated from canine cranial cruciate ligament and characterized for clonogenicity, multipotency and marker expression.
30667229	5	30	theme	extracellular	658:670	arg1	ECM					680:682	ECM	680:682	ECM	680:682	The extracellular matrix (ECM) composition was characterized by the novel application of a metabolic labeling and mass spectrometry technique.
30667229	5	30	theme	extracellular	658:670	arg1	matrix					672:677	extracellular matrix	658:677	The extracellular matrix (ECM) composition	654:695	The extracellular matrix (ECM) composition was characterized by the novel application of a metabolic labeling and mass spectrometry technique.
30667229	4	31	theme	canine	548:553	arg1	ligament					572:579	canine cranial cruciate ligament	548:579	canine cranial cruciate ligament	548:579	LPCs were isolated from canine cranial cruciate ligament and characterized for clonogenicity, multipotency and marker expression.
30667229	8	32	theme	certain	1122:1128	arg1	components					1136:1145	certain niche components	1122:1145	certain niche components	1122:1145	Metabolic labeling of cells demonstrated unique turnover profiles for distinct ECM protein groups, indicating the importance of certain niche components for LPC survival and function.
30667229	4	33	attach	isolated	534:541	arg2	LPCs					524:527	LPCs	524:527	LPCs	524:527	LPCs were isolated from canine cranial cruciate ligament and characterized for clonogenicity, multipotency and marker expression.
30667229	4	33	attach	isolated	534:541	arg1	ligament					572:579	canine cranial cruciate ligament	548:579	canine cranial cruciate ligament	548:579	LPCs were isolated from canine cranial cruciate ligament and characterized for clonogenicity, multipotency and marker expression.
30667229	1	34	theme	healing	192:198	arg1	potential					200:208	the healing potential	188:208	the healing potential of ligament	188:220	Ligaments are prone to injury and degeneration in humans and animals, however the healing potential of ligament is poor and current treatment options ineffective.
30667229	1	34	theme	healing	192:198	arg1	options					252:258	poor and current treatment options	225:258	poor and current treatment options ineffective	225:270	Ligaments are prone to injury and degeneration in humans and animals, however the healing potential of ligament is poor and current treatment options ineffective.
30667229	3	35	theme	ligament	385:392	arg1	population					416:425	a ligament progenitor cell (LPC) population	383:425	a ligament progenitor cell (LPC) population	383:425	This study aimed to characterize a ligament progenitor cell (LPC) population and to identify specific niche components which could promote the survival and function of LPCs.
30667229	3	36	dep	survival	493:500	arg1	the					489:491	the	489:491	the	489:491	This study aimed to characterize a ligament progenitor cell (LPC) population and to identify specific niche components which could promote the survival and function of LPCs.
30667229	0	37	theme	Ligament	46:53	arg1	Cells					66:70	Canine Ligament Progenitor Cells	39:70	Canine Ligament Progenitor Cells	39:70	Identification and Characterization of Canine Ligament Progenitor Cells and Their Extracellular Matrix Niche.
30667229	4	38	theme	cruciate	563:570	arg1	ligament					572:579	canine cranial cruciate ligament	548:579	canine cranial cruciate ligament	548:579	LPCs were isolated from canine cranial cruciate ligament and characterized for clonogenicity, multipotency and marker expression.
30667229	8	39	theme	components	1136:1145	arg1	importance					1108:1117	the importance	1104:1117	the importance of certain niche components for LPC survival and function	1104:1175	Metabolic labeling of cells demonstrated unique turnover profiles for distinct ECM protein groups, indicating the importance of certain niche components for LPC survival and function.
30667229	7	40	gly	glycoproteins	910:922	arg1	glycoproteins					910:922	glycoproteins	910:922	glycoproteins	910:922	A number of different collagens, glycoproteins, and proteoglycans were identified in the LPC niche using proteomics.
30667229	3	41	theme	niche	452:456	arg1	components					458:467	specific niche components	443:467	specific niche components which could promote the survival and function of LPCs	443:521	This study aimed to characterize a ligament progenitor cell (LPC) population and to identify specific niche components which could promote the survival and function of LPCs.
30667229	0	42	theme	Canine	39:44	arg1	Cells					66:70	Canine Ligament Progenitor Cells	39:70	Canine Ligament Progenitor Cells	39:70	Identification and Characterization of Canine Ligament Progenitor Cells and Their Extracellular Matrix Niche.
30667229	8	43	theme	niche	1130:1134	arg1	components					1136:1145	certain niche components	1122:1145	certain niche components	1122:1145	Metabolic labeling of cells demonstrated unique turnover profiles for distinct ECM protein groups, indicating the importance of certain niche components for LPC survival and function.
30667229	5	44	theme	metabolic	745:753	arg1	labeling					755:762	a metabolic labeling	743:762	a metabolic labeling	743:762	The extracellular matrix (ECM) composition was characterized by the novel application of a metabolic labeling and mass spectrometry technique.
30667229	1	45	theme	ligament	213:220	arg1	potential					200:208	the healing potential	188:208	the healing potential of ligament	188:220	Ligaments are prone to injury and degeneration in humans and animals, however the healing potential of ligament is poor and current treatment options ineffective.
30667229	1	45	theme	ligament	213:220	arg1	options					252:258	poor and current treatment options	225:258	poor and current treatment options ineffective	225:270	Ligaments are prone to injury and degeneration in humans and animals, however the healing potential of ligament is poor and current treatment options ineffective.
30667229	0	46	theme	Cells	66:70	arg1	Characterization					19:34	Characterization	19:34	Characterization	19:34	Identification and Characterization of Canine Ligament Progenitor Cells and Their Extracellular Matrix Niche.
30667229	0	46	theme	Cells	66:70	arg1	Identification					0:13	Identification	0:13	Identification	0:13	Identification and Characterization of Canine Ligament Progenitor Cells and Their Extracellular Matrix Niche.
30667229	8	47	theme	LPC	1151:1153	arg1	survival					1155:1162	LPC survival	1151:1162	LPC survival	1151:1162	Metabolic labeling of cells demonstrated unique turnover profiles for distinct ECM protein groups, indicating the importance of certain niche components for LPC survival and function.
30667229	3	48	theme	LPC	411:413	arg1	population					416:425	a ligament progenitor cell (LPC) population	383:425	a ligament progenitor cell (LPC) population	383:425	This study aimed to characterize a ligament progenitor cell (LPC) population and to identify specific niche components which could promote the survival and function of LPCs.
30667229	9	49	theme	LPCs	1286:1289	arg1	identification					1268:1281	identification	1268:1281	identification of LPCs	1268:1289	The newly synthesized niche components identified in this study could be exploited to aid identification of LPCs and to promote their survival and function for potential ligament repair strategies.
30667229	7	50	theme	LPC	966:968	arg1	niche					970:974	the LPC niche	962:974	the LPC niche using proteomics	962:991	A number of different collagens, glycoproteins, and proteoglycans were identified in the LPC niche using proteomics.
30667229	2	51	theme	injuries	340:347	arg1	treatment					318:326	treatment	318:326	treatment of ligament injuries	318:347	Stem cell-based therapies hold potential for treatment of ligament injuries.
30667229	6	52	theme	marker	858:863	arg1	expression					865:874	stem cell marker expression	848:874	stem cell marker expression	848:874	LPCs demonstrated clonogenicity, multipotency, and stem cell marker expression.
30667229	9	53	theme	synthesized	1188:1198	arg1	components					1206:1215	The newly synthesized niche components	1178:1215	The newly synthesized niche components identified in this study	1178:1240	The newly synthesized niche components identified in this study could be exploited to aid identification of LPCs and to promote their survival and function for potential ligament repair strategies.
30667229	2	54	theme	ligament	331:338	arg1	injuries					340:347	ligament injuries	331:347	ligament injuries	331:347	Stem cell-based therapies hold potential for treatment of ligament injuries.
30667229	1	55	theme	poor	225:228	arg1	options					252:258	poor and current treatment options	225:258	poor and current treatment options ineffective	225:270	Ligaments are prone to injury and degeneration in humans and animals, however the healing potential of ligament is poor and current treatment options ineffective.
30667229	1	55	theme	poor	225:228	arg1	potential					200:208	the healing potential	188:208	the healing potential of ligament	188:220	Ligaments are prone to injury and degeneration in humans and animals, however the healing potential of ligament is poor and current treatment options ineffective.
30667229	5	56	theme	mass	768:771	arg1	spectrometry					773:784	mass spectrometry	768:784	mass spectrometry	768:784	The extracellular matrix (ECM) composition was characterized by the novel application of a metabolic labeling and mass spectrometry technique.
30667229	6	57	theme	cell	853:856	arg1	expression					865:874	stem cell marker expression	848:874	stem cell marker expression	848:874	LPCs demonstrated clonogenicity, multipotency, and stem cell marker expression.
30667229	9	58	theme	niche	1200:1204	arg1	components					1206:1215	The newly synthesized niche components	1178:1215	The newly synthesized niche components identified in this study	1178:1240	The newly synthesized niche components identified in this study could be exploited to aid identification of LPCs and to promote their survival and function for potential ligament repair strategies.
30667229	3	59	theme	LPCs	518:521	arg1	survival					493:500	survival	493:500	survival	493:500	This study aimed to characterize a ligament progenitor cell (LPC) population and to identify specific niche components which could promote the survival and function of LPCs.
30667229	3	59	theme	LPCs	518:521	arg1	function					506:513	function	506:513	function	506:513	This study aimed to characterize a ligament progenitor cell (LPC) population and to identify specific niche components which could promote the survival and function of LPCs.
30667229	7	60	theme	proteoglycans	929:941	arg1	number					879:884	A number	877:884	A number of different collagens, glycoproteins, and proteoglycans	877:941	A number of different collagens, glycoproteins, and proteoglycans were identified in the LPC niche using proteomics.
30667229	4	61	theme	marker	635:640	arg1	expression					642:651	marker expression	635:651	marker expression	635:651	LPCs were isolated from canine cranial cruciate ligament and characterized for clonogenicity, multipotency and marker expression.
30667229	5	62	theme	spectrometry	773:784	arg1	technique					786:794	a metabolic labeling and mass spectrometry technique	743:794	a metabolic labeling and mass spectrometry technique	743:794	The extracellular matrix (ECM) composition was characterized by the novel application of a metabolic labeling and mass spectrometry technique.
30667229	8	63	theme	Metabolic	994:1002	arg1	labeling					1004:1011	Metabolic labeling	994:1011	Metabolic labeling of cells	994:1020	Metabolic labeling of cells demonstrated unique turnover profiles for distinct ECM protein groups, indicating the importance of certain niche components for LPC survival and function.
30667229	7	64	theme	glycoproteins	910:922	arg1	number					879:884	A number	877:884	A number of different collagens, glycoproteins, and proteoglycans	877:941	A number of different collagens, glycoproteins, and proteoglycans were identified in the LPC niche using proteomics.
30667229	1	65	theme	current	234:240	arg1	options					252:258	poor and current treatment options	225:258	poor and current treatment options ineffective	225:270	Ligaments are prone to injury and degeneration in humans and animals, however the healing potential of ligament is poor and current treatment options ineffective.
30667229	1	65	theme	current	234:240	arg1	potential					200:208	the healing potential	188:208	the healing potential of ligament	188:220	Ligaments are prone to injury and degeneration in humans and animals, however the healing potential of ligament is poor and current treatment options ineffective.
29944094	13	0	theme	DNA-DNA	1333:1339	arg1	data					1355:1358	DNA-DNA hybridization data	1333:1358	DNA-DNA hybridization data	1333:1358	On the basis of the polyphasic approach and DNA-DNA hybridization data, strain A301T represents a novel species within the genus Streptomyces, for which the name Streptomyces geranii sp.
29944094	4	1	theme	diagnostic	434:443	arg1	acid					453:456	the diagnostic diamino acid	430:456	the diagnostic diamino acid in the cell-wall peptidoglycan	430:487	Strain A301T contained ll-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
29944094	4	1	theme	diagnostic	434:443	arg1	acid					422:425	ll-diaminopimelic acid	404:425	ll-diaminopimelic acid	404:425	Strain A301T contained ll-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
29944094	11	2	theme	genus	1190:1194	arg1	Streptomyces					1196:1207	the genus Streptomyces	1186:1207	the genus Streptomyces	1186:1207	The DNA-DNA relatedness values between strain A301T and the two above-mentioned members of the genus Streptomyces were 42.6 % and 47.2 %, respectively.
29944094	4	3	theme	cell-wall	465:473	arg1	peptidoglycan					475:487	the cell-wall peptidoglycan	461:487	the cell-wall peptidoglycan	461:487	Strain A301T contained ll-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
29944094	13	4	theme	strain	1361:1366	arg1	A301T					1368:1372	strain A301T	1361:1372	strain A301T	1361:1372	On the basis of the polyphasic approach and DNA-DNA hybridization data, strain A301T represents a novel species within the genus Streptomyces, for which the name Streptomyces geranii sp.
29944094	13	5	theme	genus	1412:1416	arg1	Streptomyces					1418:1429	the genus Streptomyces	1408:1429	the genus Streptomyces	1408:1429	On the basis of the polyphasic approach and DNA-DNA hybridization data, strain A301T represents a novel species within the genus Streptomyces, for which the name Streptomyces geranii sp.
29944094	13	6	theme	name	1446:1449	arg1	sp					1472:1473	the name Streptomyces geranii sp	1442:1473	the name Streptomyces geranii sp	1442:1473	On the basis of the polyphasic approach and DNA-DNA hybridization data, strain A301T represents a novel species within the genus Streptomyces, for which the name Streptomyces geranii sp.
29944094	1	7	theme	endophytic	39:48	arg1	actinobacterium					50:64	a novel endophytic actinobacterium	31:64	a novel endophytic actinobacterium isolated from root of Geranium carolinianum L	31:110	nov., a novel endophytic actinobacterium isolated from root of Geranium carolinianum L.
29944094	5	8	theme	whole-cell	494:503	arg1	hydrolysates					505:516	The whole-cell hydrolysates	490:516	The whole-cell hydrolysates	490:516	The whole-cell hydrolysates included galactose and ribose.
29944094	8	9	theme	polar	697:701	arg1	profile					709:715	The polar lipid profile	693:715	The polar lipid profile	693:715	The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, two unidentified phospholipids, three unidentified lipids and two unidentified aminophospholipids.
29944094	4	10	from	acid	453:456	arg1	peptidoglycan					475:487	the cell-wall peptidoglycan	461:487	the cell-wall peptidoglycan	461:487	Strain A301T contained ll-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
29944094	13	11	theme	novel	1387:1391	arg1	species					1393:1399	a novel species	1385:1399	a novel species	1385:1399	On the basis of the polyphasic approach and DNA-DNA hybridization data, strain A301T represents a novel species within the genus Streptomyces, for which the name Streptomyces geranii sp.
29944094	9	12	theme	Strain	901:906	arg1	A301T					908:912	Strain A301T	901:912	Strain A301T	901:912	Strain A301T shared the highest 16S rRNA gene sequence similarity to Streptomyces cinereorubersubsp.
29944094	10	13	theme	Streptomyces	1044:1055	arg1	%					1091:1091	98.1 %	1086:1091	98.1 %	1086:1091	fructofermentans NBRC 15396T (98.1 %) and Streptomyces turgidiscabies ATCC 700248T (98.1 %).
29944094	10	13	theme	Streptomyces	1044:1055	arg1	ATCC					1072:1075	Streptomyces turgidiscabies ATCC 700248T	1044:1083	Streptomyces turgidiscabies ATCC 700248T (98.1 %)	1044:1092	fructofermentans NBRC 15396T (98.1 %) and Streptomyces turgidiscabies ATCC 700248T (98.1 %).
29944094	4	14	theme	diamino	445:451	arg1	acid					453:456	the diagnostic diamino acid	430:456	the diagnostic diamino acid in the cell-wall peptidoglycan	430:487	Strain A301T contained ll-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
29944094	4	14	theme	diamino	445:451	arg1	acid					422:425	ll-diaminopimelic acid	404:425	ll-diaminopimelic acid	404:425	Strain A301T contained ll-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
29944094	6	15	theme	predominant	553:563	arg1	MK-9					583:586	MK-9	583:586	MK-9	583:586	The predominant menaquinones were MK-9(H6) and MK-9(H8).
29944094	6	15	theme	predominant	553:563	arg1	menaquinones					565:576	The predominant menaquinones	549:576	The predominant menaquinones	549:576	The predominant menaquinones were MK-9(H6) and MK-9(H8).
29944094	8	16	theme	unidentified	806:817	arg1	phospholipids					819:831	two unidentified phospholipids	802:831	two unidentified phospholipids	802:831	The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, two unidentified phospholipids, three unidentified lipids and two unidentified aminophospholipids.
29944094	0	17	theme	geranii	13:19	arg1	sp					21:22	Streptomyces geranii sp	0:22	Streptomyces geranii sp.	0:23	Streptomyces geranii sp.
29944094	2	18	theme	endophytic	121:130	arg1	actinomycete					132:143	A novel endophytic actinomycete	113:143	A novel endophytic actinomycete	113:143	A novel endophytic actinomycete, designated A301T, was isolated from the root of Geranium carolinianum Linn collected from Mount Emei in China and characterized using a polyphasic approach.
29944094	15	19	theme	type	1498:1501	arg1	A301T					1513:1517	A301T	1513:1517	A301T (=CGMCC 4.7422T=JCM 32177T)	1513:1545	The type strain is A301T (=CGMCC 4.7422T=JCM 32177T).
29944094	15	19	theme	type	1498:1501	arg1	strain					1503:1508	The type strain	1494:1508	The type strain	1494:1508	The type strain is A301T (=CGMCC 4.7422T=JCM 32177T).
29944094	13	20	theme	geranii	1464:1470	arg1	sp					1472:1473	the name Streptomyces geranii sp	1442:1473	the name Streptomyces geranii sp	1442:1473	On the basis of the polyphasic approach and DNA-DNA hybridization data, strain A301T represents a novel species within the genus Streptomyces, for which the name Streptomyces geranii sp.
29944094	0	21	theme	Streptomyces	0:11	arg1	sp					21:22	Streptomyces geranii sp	0:22	Streptomyces geranii sp.	0:23	Streptomyces geranii sp.
29944094	13	22	theme	polyphasic	1309:1318	arg1	approach					1320:1327	the polyphasic approach	1305:1327	the polyphasic approach	1305:1327	On the basis of the polyphasic approach and DNA-DNA hybridization data, strain A301T represents a novel species within the genus Streptomyces, for which the name Streptomyces geranii sp.
29944094	11	23	theme	above-mentioned	1159:1173	arg1	members					1175:1181	the two above-mentioned members	1151:1181	the two above-mentioned members of the genus Streptomyces	1151:1207	The DNA-DNA relatedness values between strain A301T and the two above-mentioned members of the genus Streptomyces were 42.6 % and 47.2 %, respectively.
29944094	11	23	theme	above-mentioned	1159:1173	arg1	%					1219:1219	42.6 %	1214:1219	42.6 %	1214:1219	The DNA-DNA relatedness values between strain A301T and the two above-mentioned members of the genus Streptomyces were 42.6 % and 47.2 %, respectively.
29944094	2	24	dep	Emei	242:245	arg1	Mount					236:240	Mount	236:240	Mount	236:240	A novel endophytic actinomycete, designated A301T, was isolated from the root of Geranium carolinianum Linn collected from Mount Emei in China and characterized using a polyphasic approach.
29944094	9	25	theme	highest	925:931	arg1	similarity					956:965	the highest 16S rRNA gene sequence similarity	921:965	the highest 16S rRNA gene sequence similarity to Streptomyces cinereorubersubsp	921:999	Strain A301T shared the highest 16S rRNA gene sequence similarity to Streptomyces cinereorubersubsp.
29944094	2	26	theme	polyphasic	282:291	arg1	approach					293:300	a polyphasic approach	280:300	a polyphasic approach	280:300	A novel endophytic actinomycete, designated A301T, was isolated from the root of Geranium carolinianum Linn collected from Mount Emei in China and characterized using a polyphasic approach.
29944094	9	27	theme	rRNA	937:940	arg1	similarity					956:965	the highest 16S rRNA gene sequence similarity	921:965	the highest 16S rRNA gene sequence similarity to Streptomyces cinereorubersubsp	921:999	Strain A301T shared the highest 16S rRNA gene sequence similarity to Streptomyces cinereorubersubsp.
29944094	9	28	theme	16S	933:935	arg1	similarity					956:965	the highest 16S rRNA gene sequence similarity	921:965	the highest 16S rRNA gene sequence similarity to Streptomyces cinereorubersubsp	921:999	Strain A301T shared the highest 16S rRNA gene sequence similarity to Streptomyces cinereorubersubsp.
29944094	9	29	theme	Streptomyces	970:981	arg1	cinereorubersubsp					983:999	Streptomyces cinereorubersubsp	970:999	Streptomyces cinereorubersubsp	970:999	Strain A301T shared the highest 16S rRNA gene sequence similarity to Streptomyces cinereorubersubsp.
29944094	1	30	theme	carolinianum	97:108	arg1	L					110:110	Geranium carolinianum L	88:110	Geranium carolinianum L	88:110	nov., a novel endophytic actinobacterium isolated from root of Geranium carolinianum L.
29944094	8	31	theme	unidentified	840:851	arg1	lipids					853:858	three unidentified lipids	834:858	three unidentified lipids	834:858	The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, two unidentified phospholipids, three unidentified lipids and two unidentified aminophospholipids.
29944094	2	32	attach	isolated	168:175	arg2	actinomycete					132:143	A novel endophytic actinomycete	113:143	A novel endophytic actinomycete	113:143	A novel endophytic actinomycete, designated A301T, was isolated from the root of Geranium carolinianum Linn collected from Mount Emei in China and characterized using a polyphasic approach.
29944094	2	32	attach	isolated	168:175	arg1	root					186:189	the root	182:189	the root of Geranium carolinianum Linn collected from Mount Emei in China	182:254	A novel endophytic actinomycete, designated A301T, was isolated from the root of Geranium carolinianum Linn collected from Mount Emei in China and characterized using a polyphasic approach.
29944094	3	33	theme	 NaCl	368:372	arg1	presence					351:358	the presence	347:358	the presence of 0-5 % NaCl (w/v)	347:378	Growth occurred at 10-37 °C, pH 6-11 and in the presence of 0-5 % NaCl (w/v).
29944094	1	34	attach	isolated	66:73	arg1	root					80:83	root	80:83	root of Geranium carolinianum L	80:110	nov., a novel endophytic actinobacterium isolated from root of Geranium carolinianum L.
29944094	1	34	attach	isolated	66:73	arg2	actinobacterium					50:64	a novel endophytic actinobacterium	31:64	a novel endophytic actinobacterium isolated from root of Geranium carolinianum L	31:110	nov., a novel endophytic actinobacterium isolated from root of Geranium carolinianum L.
29944094	2	35	from	Emei	242:245	arg1	China					250:254	China	250:254	China	250:254	A novel endophytic actinomycete, designated A301T, was isolated from the root of Geranium carolinianum Linn collected from Mount Emei in China and characterized using a polyphasic approach.
29944094	9	36	theme	gene	942:945	arg1	similarity					956:965	the highest 16S rRNA gene sequence similarity	921:965	the highest 16S rRNA gene sequence similarity to Streptomyces cinereorubersubsp	921:999	Strain A301T shared the highest 16S rRNA gene sequence similarity to Streptomyces cinereorubersubsp.
29944094	2	37	theme	Linn	216:219	arg1	root					186:189	the root	182:189	the root of Geranium carolinianum Linn collected from Mount Emei in China	182:254	A novel endophytic actinomycete, designated A301T, was isolated from the root of Geranium carolinianum Linn collected from Mount Emei in China and characterized using a polyphasic approach.
29944094	4	38	contain	contained	394:402	arg2	acid					422:425	ll-diaminopimelic acid	404:425	ll-diaminopimelic acid	404:425	Strain A301T contained ll-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
29944094	4	38	contain	contained	394:402	arg2	acid					453:456	the diagnostic diamino acid	430:456	the diagnostic diamino acid in the cell-wall peptidoglycan	430:487	Strain A301T contained ll-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
29944094	4	38	contain	contained	394:402	arg1	A301T					388:392	Strain A301T	381:392	Strain A301T	381:392	Strain A301T contained ll-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
29944094	3	39	theme	0-5 	363:366	arg1	w/v					375:377	w/v	375:377	w/v	375:377	Growth occurred at 10-37 °C, pH 6-11 and in the presence of 0-5 % NaCl (w/v).
29944094	3	39	theme	0-5 	363:366	arg1	 NaCl					368:372	0-5 % NaCl	363:372	0-5 % NaCl (w/v)	363:378	Growth occurred at 10-37 °C, pH 6-11 and in the presence of 0-5 % NaCl (w/v).
29944094	4	40	theme	ll-diaminopimelic	404:420	arg1	acid					453:456	the diagnostic diamino acid	430:456	the diagnostic diamino acid in the cell-wall peptidoglycan	430:487	Strain A301T contained ll-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
29944094	4	40	theme	ll-diaminopimelic	404:420	arg1	acid					422:425	ll-diaminopimelic acid	404:425	ll-diaminopimelic acid	404:425	Strain A301T contained ll-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
29944094	10	41	theme	turgidiscabies	1057:1070	arg1	%					1091:1091	98.1 %	1086:1091	98.1 %	1086:1091	fructofermentans NBRC 15396T (98.1 %) and Streptomyces turgidiscabies ATCC 700248T (98.1 %).
29944094	10	41	theme	turgidiscabies	1057:1070	arg1	ATCC					1072:1075	Streptomyces turgidiscabies ATCC 700248T	1044:1083	Streptomyces turgidiscabies ATCC 700248T (98.1 %)	1044:1092	fructofermentans NBRC 15396T (98.1 %) and Streptomyces turgidiscabies ATCC 700248T (98.1 %).
29944094	1	42	theme	L	110:110	arg1	root					80:83	root	80:83	root of Geranium carolinianum L	80:110	nov., a novel endophytic actinobacterium isolated from root of Geranium carolinianum L.
29944094	3	43	theme	%	367:367	arg1	w/v					375:377	w/v	375:377	w/v	375:377	Growth occurred at 10-37 °C, pH 6-11 and in the presence of 0-5 % NaCl (w/v).
29944094	3	43	theme	%	367:367	arg1	 NaCl					368:372	0-5 % NaCl	363:372	0-5 % NaCl (w/v)	363:378	Growth occurred at 10-37 °C, pH 6-11 and in the presence of 0-5 % NaCl (w/v).
29944094	7	44	theme	cellular	616:623	arg1	C15 					642:645	C15 	642:645	C15 	642:645	The major cellular fatty acids were C15 : 0, C16 : 0, anteiso-C15 : 0 and iso-C16 : 0.
29944094	7	44	theme	cellular	616:623	arg1	acids					631:635	The major cellular fatty acids	606:635	The major cellular fatty acids	606:635	The major cellular fatty acids were C15 : 0, C16 : 0, anteiso-C15 : 0 and iso-C16 : 0.
29944094	8	45	theme	unidentified	868:879	arg1	aminophospholipids					881:898	two unidentified aminophospholipids	864:898	two unidentified aminophospholipids	864:898	The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, two unidentified phospholipids, three unidentified lipids and two unidentified aminophospholipids.
29944094	13	46	dep	approach	1320:1327	arg1	the					1292:1294	the	1292:1294	the	1292:1294	On the basis of the polyphasic approach and DNA-DNA hybridization data, strain A301T represents a novel species within the genus Streptomyces, for which the name Streptomyces geranii sp.
29944094	13	46	dep	approach	1320:1327	arg1	basis					1296:1300	basis	1296:1300	basis	1296:1300	On the basis of the polyphasic approach and DNA-DNA hybridization data, strain A301T represents a novel species within the genus Streptomyces, for which the name Streptomyces geranii sp.
29944094	12	47	theme	DNA	1270:1272	arg1	%					1286:1286	70.5 mol%	1278:1286	70.5 mol%	1278:1286	The G+C content of the DNA was 70.5 mol%.
29944094	12	47	theme	DNA	1270:1272	arg1	G+C content					1251:1261	The G+C content	1247:1261	The G+C content of the DNA	1247:1272	The G+C content of the DNA was 70.5 mol%.
29944094	11	48	theme	Streptomyces	1196:1207	arg1	values					1119:1124	The DNA-DNA relatedness values	1095:1124	The DNA-DNA relatedness values between strain A301T	1095:1145	The DNA-DNA relatedness values between strain A301T and the two above-mentioned members of the genus Streptomyces were 42.6 % and 47.2 %, respectively.
29944094	11	48	theme	Streptomyces	1196:1207	arg1	members					1175:1181	the two above-mentioned members	1151:1181	the two above-mentioned members of the genus Streptomyces	1151:1207	The DNA-DNA relatedness values between strain A301T and the two above-mentioned members of the genus Streptomyces were 42.6 % and 47.2 %, respectively.
29944094	11	48	theme	Streptomyces	1196:1207	arg1	%					1219:1219	42.6 %	1214:1219	42.6 %	1214:1219	The DNA-DNA relatedness values between strain A301T and the two above-mentioned members of the genus Streptomyces were 42.6 % and 47.2 %, respectively.
29944094	7	49	theme	fatty	625:629	arg1	C15 					642:645	C15 	642:645	C15 	642:645	The major cellular fatty acids were C15 : 0, C16 : 0, anteiso-C15 : 0 and iso-C16 : 0.
29944094	7	49	theme	fatty	625:629	arg1	acids					631:635	The major cellular fatty acids	606:635	The major cellular fatty acids	606:635	The major cellular fatty acids were C15 : 0, C16 : 0, anteiso-C15 : 0 and iso-C16 : 0.
29944094	11	50	theme	DNA-DNA	1099:1105	arg1	values					1119:1124	The DNA-DNA relatedness values	1095:1124	The DNA-DNA relatedness values between strain A301T	1095:1145	The DNA-DNA relatedness values between strain A301T and the two above-mentioned members of the genus Streptomyces were 42.6 % and 47.2 %, respectively.
29944094	11	50	theme	DNA-DNA	1099:1105	arg1	%					1219:1219	42.6 %	1214:1219	42.6 %	1214:1219	The DNA-DNA relatedness values between strain A301T and the two above-mentioned members of the genus Streptomyces were 42.6 % and 47.2 %, respectively.
29944094	12	51	theme	70.5 mol	1278:1285	arg1	%					1286:1286	70.5 mol%	1278:1286	70.5 mol%	1278:1286	The G+C content of the DNA was 70.5 mol%.
29944094	12	51	theme	70.5 mol	1278:1285	arg1	G+C content					1251:1261	The G+C content	1247:1261	The G+C content of the DNA	1247:1272	The G+C content of the DNA was 70.5 mol%.
29944094	15	52	theme	4.7422T=JCM	1527:1537	arg1	32177T					1539:1544	=CGMCC 4.7422T=JCM 32177T	1520:1544	=CGMCC 4.7422T=JCM 32177T	1520:1544	The type strain is A301T (=CGMCC 4.7422T=JCM 32177T).
29944094	15	52	theme	4.7422T=JCM	1527:1537	arg1	A301T					1513:1517	A301T	1513:1517	A301T (=CGMCC 4.7422T=JCM 32177T)	1513:1545	The type strain is A301T (=CGMCC 4.7422T=JCM 32177T).
29944094	1	53	theme	Geranium	88:95	arg1	L					110:110	Geranium carolinianum L	88:110	Geranium carolinianum L	88:110	nov., a novel endophytic actinobacterium isolated from root of Geranium carolinianum L.
29944094	4	54	theme	Strain	381:386	arg1	A301T					388:392	Strain A301T	381:392	Strain A301T	381:392	Strain A301T contained ll-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
29944094	11	55	theme	relatedness	1107:1117	arg1	values					1119:1124	The DNA-DNA relatedness values	1095:1124	The DNA-DNA relatedness values between strain A301T	1095:1145	The DNA-DNA relatedness values between strain A301T and the two above-mentioned members of the genus Streptomyces were 42.6 % and 47.2 %, respectively.
29944094	11	55	theme	relatedness	1107:1117	arg1	%					1219:1219	42.6 %	1214:1219	42.6 %	1214:1219	The DNA-DNA relatedness values between strain A301T and the two above-mentioned members of the genus Streptomyces were 42.6 % and 47.2 %, respectively.
29944094	11	56	theme	strain	1134:1139	arg1	A301T					1141:1145	strain A301T	1134:1145	strain A301T	1134:1145	The DNA-DNA relatedness values between strain A301T and the two above-mentioned members of the genus Streptomyces were 42.6 % and 47.2 %, respectively.
29944094	1	57	dep	actinobacterium	50:64	arg1	nov.					25:28	nov.	25:28	nov.	25:28	nov., a novel endophytic actinobacterium isolated from root of Geranium carolinianum L.
29944094	7	58	theme	major	610:614	arg1	C15 					642:645	C15 	642:645	C15 	642:645	The major cellular fatty acids were C15 : 0, C16 : 0, anteiso-C15 : 0 and iso-C16 : 0.
29944094	7	58	theme	major	610:614	arg1	acids					631:635	The major cellular fatty acids	606:635	The major cellular fatty acids	606:635	The major cellular fatty acids were C15 : 0, C16 : 0, anteiso-C15 : 0 and iso-C16 : 0.
29944094	13	59	theme	Streptomyces	1451:1462	arg1	sp					1472:1473	the name Streptomyces geranii sp	1442:1473	the name Streptomyces geranii sp	1442:1473	On the basis of the polyphasic approach and DNA-DNA hybridization data, strain A301T represents a novel species within the genus Streptomyces, for which the name Streptomyces geranii sp.
29944094	13	60	theme	hybridization	1341:1353	arg1	data					1355:1358	DNA-DNA hybridization data	1333:1358	DNA-DNA hybridization data	1333:1358	On the basis of the polyphasic approach and DNA-DNA hybridization data, strain A301T represents a novel species within the genus Streptomyces, for which the name Streptomyces geranii sp.
29944094	8	61	theme	lipid	703:707	arg1	profile					709:715	The polar lipid profile	693:715	The polar lipid profile	693:715	The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, two unidentified phospholipids, three unidentified lipids and two unidentified aminophospholipids.
29944094	7	62	dep	C15 	642:645	arg1	iso-C16 					680:687	iso-C16 	680:687	iso-C16 	680:687	The major cellular fatty acids were C15 : 0, C16 : 0, anteiso-C15 : 0 and iso-C16 : 0.
29944094	7	62	dep	C15 	642:645	arg1	C16 					651:654	C16 	651:654	C16 	651:654	The major cellular fatty acids were C15 : 0, C16 : 0, anteiso-C15 : 0 and iso-C16 : 0.
29944094	7	62	dep	C15 	642:645	arg1	 0					673:674	 0	673:674	 0	673:674	The major cellular fatty acids were C15 : 0, C16 : 0, anteiso-C15 : 0 and iso-C16 : 0.
29944094	7	62	dep	C15 	642:645	arg1	0					657:657	0	657:657	0	657:657	The major cellular fatty acids were C15 : 0, C16 : 0, anteiso-C15 : 0 and iso-C16 : 0.
29944094	7	62	dep	C15 	642:645	arg1	 0					689:690	 0	689:690	C15 : 0, C16 : 0, anteiso-C15 : 0 and iso-C16 : 0	642:690	The major cellular fatty acids were C15 : 0, C16 : 0, anteiso-C15 : 0 and iso-C16 : 0.
29944094	9	63	theme	sequence	947:954	arg1	similarity					956:965	the highest 16S rRNA gene sequence similarity	921:965	the highest 16S rRNA gene sequence similarity to Streptomyces cinereorubersubsp	921:999	Strain A301T shared the highest 16S rRNA gene sequence similarity to Streptomyces cinereorubersubsp.
29944094	2	64	theme	novel	115:119	arg1	actinomycete					132:143	A novel endophytic actinomycete	113:143	A novel endophytic actinomycete	113:143	A novel endophytic actinomycete, designated A301T, was isolated from the root of Geranium carolinianum Linn collected from Mount Emei in China and characterized using a polyphasic approach.
29944094	1	65	theme	novel	33:37	arg1	actinobacterium					50:64	a novel endophytic actinobacterium	31:64	a novel endophytic actinobacterium isolated from root of Geranium carolinianum L	31:110	nov., a novel endophytic actinobacterium isolated from root of Geranium carolinianum L.
29944094	15	66	theme	=CGMCC	1520:1525	arg1	32177T					1539:1544	=CGMCC 4.7422T=JCM 32177T	1520:1544	=CGMCC 4.7422T=JCM 32177T	1520:1544	The type strain is A301T (=CGMCC 4.7422T=JCM 32177T).
29944094	15	66	theme	=CGMCC	1520:1525	arg1	A301T					1513:1517	A301T	1513:1517	A301T (=CGMCC 4.7422T=JCM 32177T)	1513:1545	The type strain is A301T (=CGMCC 4.7422T=JCM 32177T).
30431422	3	0	theme	strain	317:322	arg1	KNUC7312T					324:332	strain KNUC7312T	317:332	strain KNUC7312T	317:332	Cell growth of strain KNUC7312T occurred at 10-45 °C (optimum, 30 °C) and pH 7-12 (optimum, pH 7).
30431422	10	1	theme	DNA-DNA	1150:1156	arg1	value					1170:1174	a low DNA-DNA relatedness value	1144:1174	a low DNA-DNA relatedness value (47.36 %)	1144:1184	Strain KNUC7312T showed a low DNA-DNA relatedness value (47.36 %) with B. humi DSM 16318T, which supported that this strain represents a novel Bacillusspecies.
30431422	10	1	theme	DNA-DNA	1150:1156	arg1	%					1183:1183	47.36 %	1177:1183	47.36 %	1177:1183	Strain KNUC7312T showed a low DNA-DNA relatedness value (47.36 %) with B. humi DSM 16318T, which supported that this strain represents a novel Bacillusspecies.
30431422	11	2	theme	chemotaxonomic	1308:1321	arg1	evidence					1340:1347	phenotypic, chemotaxonomic and phylogenetic evidence	1296:1347	phenotypic, chemotaxonomic and phylogenetic evidence	1296:1347	On the basis of phenotypic, chemotaxonomic and phylogenetic evidence, strain KNUC7312T represents a novel species within the genera Bacillus.
30431422	5	3	theme	other	612:616	arg1	species					618:624	other species	612:624	other species of the genus Bacillus	612:646	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain KNUC7312T clustered together with other species of the genus Bacillus and was most closely related to Bacillus humi DSM 16318T (98.0 %).
30431422	7	4	theme	major	781:785	arg1	acids					802:806	The major cellular fatty acids	777:806	The major cellular fatty acids	777:806	The major cellular fatty acids were anteiso C15 : 0, iso-C15 : 0 and iso-C14 : 0.
30431422	7	4	theme	major	781:785	arg1	C15 					821:824	C15 	821:824	C15 	821:824	The major cellular fatty acids were anteiso C15 : 0, iso-C15 : 0 and iso-C14 : 0.
30431422	3	5	theme	KNUC7312T	324:332	arg1	growth					307:312	Cell growth	302:312	Cell growth of strain KNUC7312T	302:332	Cell growth of strain KNUC7312T occurred at 10-45 °C (optimum, 30 °C) and pH 7-12 (optimum, pH 7).
30431422	4	6	theme	 NaCl	453:457	arg1	optimum					480:486	optimum	480:486	optimum	480:486	In addition, this strain was able to tolerate 0-12 % NaCl (w/v) concentration (optimum, 0-1 %).
30431422	4	6	theme	 NaCl	453:457	arg1	concentration					465:477	0-12 % NaCl (w/v) concentration	447:477	0-12 % NaCl (w/v) concentration (optimum, 0-1 %)	447:494	In addition, this strain was able to tolerate 0-12 % NaCl (w/v) concentration (optimum, 0-1 %).
30431422	9	7	theme	diagnostic	1095:1104	arg1	acid					1114:1117	the major diagnostic diamino acid	1085:1117	the major diagnostic diamino acid	1085:1117	The cell-wall peptidoglycan contained meso-diaminopimelic acid as the major diagnostic diamino acid.
30431422	9	7	theme	diagnostic	1095:1104	arg1	acid					1077:1080	meso-diaminopimelic acid	1057:1080	meso-diaminopimelic acid	1057:1080	The cell-wall peptidoglycan contained meso-diaminopimelic acid as the major diagnostic diamino acid.
30431422	12	8	theme	Bacillus	1431:1438	arg1	salildurans					1440:1450	The name Bacillus salildurans	1422:1450	The name Bacillus salildurans	1422:1450	The name Bacillus salildurans sp.
30431422	5	9	theme	Bacillus	639:646	arg1	species					618:624	other species	612:624	other species of the genus Bacillus	612:646	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain KNUC7312T clustered together with other species of the genus Bacillus and was most closely related to Bacillus humi DSM 16318T (98.0 %).
30431422	4	10	theme	%	452:452	arg1	w/v					460:462	w/v	460:462	w/v	460:462	In addition, this strain was able to tolerate 0-12 % NaCl (w/v) concentration (optimum, 0-1 %).
30431422	4	10	theme	%	452:452	arg1	 NaCl					453:457	% NaCl	452:457	0-12 % NaCl (w/v) concentration (optimum, 0-1 %)	447:494	In addition, this strain was able to tolerate 0-12 % NaCl (w/v) concentration (optimum, 0-1 %).
30431422	5	11	theme	strain	571:576	arg1	KNUC7312T					578:586	the strain KNUC7312T	567:586	the strain KNUC7312T	567:586	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain KNUC7312T clustered together with other species of the genus Bacillus and was most closely related to Bacillus humi DSM 16318T (98.0 %).
30431422	9	12	theme	diamino	1106:1112	arg1	acid					1114:1117	the major diagnostic diamino acid	1085:1117	the major diagnostic diamino acid	1085:1117	The cell-wall peptidoglycan contained meso-diaminopimelic acid as the major diagnostic diamino acid.
30431422	9	12	theme	diamino	1106:1112	arg1	acid					1077:1080	meso-diaminopimelic acid	1057:1080	meso-diaminopimelic acid	1057:1080	The cell-wall peptidoglycan contained meso-diaminopimelic acid as the major diagnostic diamino acid.
30431422	1	13	theme	salt-accumulated	44:59	arg1	soil					81:84	salt-accumulated pepper rhizospheric soil	44:84	salt-accumulated pepper rhizospheric soil	44:84	nov., isolated from salt-accumulated pepper rhizospheric soil.
30431422	7	14	dep	 0	826:827	arg1	 0					855:856	 0	855:856	 0	855:856	The major cellular fatty acids were anteiso C15 : 0, iso-C15 : 0 and iso-C14 : 0.
30431422	7	14	dep	 0	826:827	arg1	iso-C14 					846:853	iso-C14 	846:853	iso-C14 	846:853	The major cellular fatty acids were anteiso C15 : 0, iso-C15 : 0 and iso-C14 : 0.
30431422	7	14	dep	 0	826:827	arg1	 0					839:840	 0	839:840	 0	839:840	The major cellular fatty acids were anteiso C15 : 0, iso-C15 : 0 and iso-C14 : 0.
30431422	11	15	dep	evidence	1340:1347	arg1	basis					1287:1291	basis	1287:1291	basis	1287:1291	On the basis of phenotypic, chemotaxonomic and phylogenetic evidence, strain KNUC7312T represents a novel species within the genera Bacillus.
30431422	11	15	dep	evidence	1340:1347	arg1	the					1283:1285	the	1283:1285	the	1283:1285	On the basis of phenotypic, chemotaxonomic and phylogenetic evidence, strain KNUC7312T represents a novel species within the genera Bacillus.
30431422	1	16	theme	pepper	61:66	arg1	soil					81:84	salt-accumulated pepper rhizospheric soil	44:84	salt-accumulated pepper rhizospheric soil	44:84	nov., isolated from salt-accumulated pepper rhizospheric soil.
30431422	10	17	theme	DSM	1199:1201	arg1	16318T					1203:1208	B. humi DSM 16318T	1191:1208	B. humi DSM 16318T	1191:1208	Strain KNUC7312T showed a low DNA-DNA relatedness value (47.36 %) with B. humi DSM 16318T, which supported that this strain represents a novel Bacillusspecies.
30431422	10	18	theme	Strain	1120:1125	arg1	KNUC7312T					1127:1135	Strain KNUC7312T	1120:1135	Strain KNUC7312T	1120:1135	Strain KNUC7312T showed a low DNA-DNA relatedness value (47.36 %) with B. humi DSM 16318T, which supported that this strain represents a novel Bacillusspecies.
30431422	11	19	theme	phenotypic	1296:1305	arg1	evidence					1340:1347	phenotypic, chemotaxonomic and phylogenetic evidence	1296:1347	phenotypic, chemotaxonomic and phylogenetic evidence	1296:1347	On the basis of phenotypic, chemotaxonomic and phylogenetic evidence, strain KNUC7312T represents a novel species within the genera Bacillus.
30431422	5	20	theme	rRNA	532:535	arg1	sequences					542:550	16S rRNA gene sequences	528:550	16S rRNA gene sequences	528:550	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain KNUC7312T clustered together with other species of the genus Bacillus and was most closely related to Bacillus humi DSM 16318T (98.0 %).
30431422	5	21	theme	16S	528:530	arg1	sequences					542:550	16S rRNA gene sequences	528:550	16S rRNA gene sequences	528:550	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain KNUC7312T clustered together with other species of the genus Bacillus and was most closely related to Bacillus humi DSM 16318T (98.0 %).
30431422	1	22	theme	rhizospheric	68:79	arg1	soil					81:84	salt-accumulated pepper rhizospheric soil	44:84	salt-accumulated pepper rhizospheric soil	44:84	nov., isolated from salt-accumulated pepper rhizospheric soil.
30431422	2	23	from	soil	238:241	arg1	greenhouse					255:264	a pepper greenhouse	246:264	a pepper greenhouse in Miryang city, Republic of Korea	246:299	A Gram-stain-positive, facultatively anaerobic, motile and rod-shaped bacterium, designated KNUC7312T, was isolated from salt-accumulated rhizospheric soil in a pepper greenhouse in Miryang city, Republic of Korea.
30431422	2	24	theme	rod-shaped	146:155	arg1	bacterium					157:165	A Gram-stain-positive, facultatively anaerobic, motile and rod-shaped bacterium	87:165	A Gram-stain-positive, facultatively anaerobic, motile and rod-shaped bacterium	87:165	A Gram-stain-positive, facultatively anaerobic, motile and rod-shaped bacterium, designated KNUC7312T, was isolated from salt-accumulated rhizospheric soil in a pepper greenhouse in Miryang city, Republic of Korea.
30431422	4	25	from	able	430:433	arg1	addition					404:411	addition	404:411	addition	404:411	In addition, this strain was able to tolerate 0-12 % NaCl (w/v) concentration (optimum, 0-1 %).
30431422	0	26	theme	Bacillus	0:7	arg1	sp					20:21	Bacillus salidurans sp	0:21	Bacillus salidurans sp.	0:22	Bacillus salidurans sp.
30431422	10	27	theme	B.	1191:1192	arg1	16318T					1203:1208	B. humi DSM 16318T	1191:1208	B. humi DSM 16318T	1191:1208	Strain KNUC7312T showed a low DNA-DNA relatedness value (47.36 %) with B. humi DSM 16318T, which supported that this strain represents a novel Bacillusspecies.
30431422	11	28	theme	strain	1350:1355	arg1	KNUC7312T					1357:1365	strain KNUC7312T	1350:1365	strain KNUC7312T	1350:1365	On the basis of phenotypic, chemotaxonomic and phylogenetic evidence, strain KNUC7312T represents a novel species within the genera Bacillus.
30431422	0	29	theme	salidurans	9:18	arg1	sp					20:21	Bacillus salidurans sp	0:21	Bacillus salidurans sp.	0:22	Bacillus salidurans sp.
30431422	5	30	theme	Bacillus	680:687	arg1	16318T					698:703	Bacillus humi DSM 16318T	680:703	Bacillus humi DSM 16318T	680:703	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain KNUC7312T clustered together with other species of the genus Bacillus and was most closely related to Bacillus humi DSM 16318T (98.0 %).
30431422	3	31	dep	optimum	385:391	arg1	pH					394:395	pH 7	394:397	pH 7	394:397	Cell growth of strain KNUC7312T occurred at 10-45 °C (optimum, 30 °C) and pH 7-12 (optimum, pH 7).
30431422	9	32	theme	major	1089:1093	arg1	acid					1114:1117	the major diagnostic diamino acid	1085:1117	the major diagnostic diamino acid	1085:1117	The cell-wall peptidoglycan contained meso-diaminopimelic acid as the major diagnostic diamino acid.
30431422	9	32	theme	major	1089:1093	arg1	acid					1077:1080	meso-diaminopimelic acid	1057:1080	meso-diaminopimelic acid	1057:1080	The cell-wall peptidoglycan contained meso-diaminopimelic acid as the major diagnostic diamino acid.
30431422	8	33	theme	unidentified	993:1004	arg1	aminolipids					1006:1016	two unidentified aminolipids	989:1016	two unidentified aminolipids	989:1016	The polar lipid profile contained the major components diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and two unidentified aminolipids.
30431422	2	34	attach	isolated	194:201	arg1	soil					238:241	salt-accumulated rhizospheric soil	208:241	salt-accumulated rhizospheric soil in a pepper greenhouse in Miryang city, Republic of Korea	208:299	A Gram-stain-positive, facultatively anaerobic, motile and rod-shaped bacterium, designated KNUC7312T, was isolated from salt-accumulated rhizospheric soil in a pepper greenhouse in Miryang city, Republic of Korea.
30431422	2	34	attach	isolated	194:201	arg2	bacterium					157:165	A Gram-stain-positive, facultatively anaerobic, motile and rod-shaped bacterium	87:165	A Gram-stain-positive, facultatively anaerobic, motile and rod-shaped bacterium	87:165	A Gram-stain-positive, facultatively anaerobic, motile and rod-shaped bacterium, designated KNUC7312T, was isolated from salt-accumulated rhizospheric soil in a pepper greenhouse in Miryang city, Republic of Korea.
30431422	2	35	theme	anaerobic	124:132	arg1	bacterium					157:165	A Gram-stain-positive, facultatively anaerobic, motile and rod-shaped bacterium	87:165	A Gram-stain-positive, facultatively anaerobic, motile and rod-shaped bacterium	87:165	A Gram-stain-positive, facultatively anaerobic, motile and rod-shaped bacterium, designated KNUC7312T, was isolated from salt-accumulated rhizospheric soil in a pepper greenhouse in Miryang city, Republic of Korea.
30431422	8	36	theme	lipid	869:873	arg1	profile					875:881	The polar lipid profile	859:881	The polar lipid profile	859:881	The polar lipid profile contained the major components diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and two unidentified aminolipids.
30431422	14	37	theme	type	1478:1481	arg1	strain					1483:1488	The type strain	1474:1488	The type strain	1474:1488	The type strain is KNUC7312T (KCTC 33852T=CGMCC 1.13629T).
30431422	14	37	theme	type	1478:1481	arg1	KNUC7312T					1493:1501	KNUC7312T	1493:1501	KNUC7312T (KCTC 33852T=CGMCC 1.13629T)	1493:1530	The type strain is KNUC7312T (KCTC 33852T=CGMCC 1.13629T).
30431422	9	38	contain	contained	1047:1055	arg2	acid					1114:1117	the major diagnostic diamino acid	1085:1117	the major diagnostic diamino acid	1085:1117	The cell-wall peptidoglycan contained meso-diaminopimelic acid as the major diagnostic diamino acid.
30431422	9	38	contain	contained	1047:1055	arg1	peptidoglycan					1033:1045	The cell-wall peptidoglycan	1019:1045	The cell-wall peptidoglycan	1019:1045	The cell-wall peptidoglycan contained meso-diaminopimelic acid as the major diagnostic diamino acid.
30431422	9	38	contain	contained	1047:1055	arg2	acid					1077:1080	meso-diaminopimelic acid	1057:1080	meso-diaminopimelic acid	1057:1080	The cell-wall peptidoglycan contained meso-diaminopimelic acid as the major diagnostic diamino acid.
30431422	5	39	theme	Phylogenetic	497:508	arg1	analysis					510:517	Phylogenetic analysis	497:517	Phylogenetic analysis based on 16S rRNA gene sequences	497:550	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain KNUC7312T clustered together with other species of the genus Bacillus and was most closely related to Bacillus humi DSM 16318T (98.0 %).
30431422	2	40	from	greenhouse	255:264	arg1	Republic					283:290	Republic	283:290	Republic	283:290	A Gram-stain-positive, facultatively anaerobic, motile and rod-shaped bacterium, designated KNUC7312T, was isolated from salt-accumulated rhizospheric soil in a pepper greenhouse in Miryang city, Republic of Korea.
30431422	11	41	dep	genera	1405:1410	arg1	Bacillus					1412:1419	the genera Bacillus	1401:1419	the genera Bacillus	1401:1419	On the basis of phenotypic, chemotaxonomic and phylogenetic evidence, strain KNUC7312T represents a novel species within the genera Bacillus.
30431422	5	42	theme	humi	689:692	arg1	16318T					698:703	Bacillus humi DSM 16318T	680:703	Bacillus humi DSM 16318T	680:703	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain KNUC7312T clustered together with other species of the genus Bacillus and was most closely related to Bacillus humi DSM 16318T (98.0 %).
30431422	10	43	theme	humi	1194:1197	arg1	16318T					1203:1208	B. humi DSM 16318T	1191:1208	B. humi DSM 16318T	1191:1208	Strain KNUC7312T showed a low DNA-DNA relatedness value (47.36 %) with B. humi DSM 16318T, which supported that this strain represents a novel Bacillusspecies.
30431422	4	44	from	addition	404:411	arg1	able					430:433	able	430:433	able	430:433	In addition, this strain was able to tolerate 0-12 % NaCl (w/v) concentration (optimum, 0-1 %).
30431422	12	45	dep	sp	1452:1453	arg1	salildurans					1440:1450	The name Bacillus salildurans	1422:1450	The name Bacillus salildurans	1422:1450	The name Bacillus salildurans sp.
30431422	2	46	theme	pepper	248:253	arg1	greenhouse					255:264	a pepper greenhouse	246:264	a pepper greenhouse in Miryang city, Republic of Korea	246:299	A Gram-stain-positive, facultatively anaerobic, motile and rod-shaped bacterium, designated KNUC7312T, was isolated from salt-accumulated rhizospheric soil in a pepper greenhouse in Miryang city, Republic of Korea.
30431422	10	47	theme	low	1146:1148	arg1	value					1170:1174	a low DNA-DNA relatedness value	1144:1174	a low DNA-DNA relatedness value (47.36 %)	1144:1184	Strain KNUC7312T showed a low DNA-DNA relatedness value (47.36 %) with B. humi DSM 16318T, which supported that this strain represents a novel Bacillusspecies.
30431422	10	47	theme	low	1146:1148	arg1	%					1183:1183	47.36 %	1177:1183	47.36 %	1177:1183	Strain KNUC7312T showed a low DNA-DNA relatedness value (47.36 %) with B. humi DSM 16318T, which supported that this strain represents a novel Bacillusspecies.
30431422	5	48	theme	DSM	694:696	arg1	16318T					698:703	Bacillus humi DSM 16318T	680:703	Bacillus humi DSM 16318T	680:703	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain KNUC7312T clustered together with other species of the genus Bacillus and was most closely related to Bacillus humi DSM 16318T (98.0 %).
30431422	5	49	dep	related	669:675	arg1	%					711:711	98.0 %	706:711	98.0 %	706:711	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain KNUC7312T clustered together with other species of the genus Bacillus and was most closely related to Bacillus humi DSM 16318T (98.0 %).
30431422	12	50	theme	name	1426:1429	arg1	salildurans					1440:1450	The name Bacillus salildurans	1422:1450	The name Bacillus salildurans	1422:1450	The name Bacillus salildurans sp.
30431422	9	51	theme	cell-wall	1023:1031	arg1	peptidoglycan					1033:1045	The cell-wall peptidoglycan	1019:1045	The cell-wall peptidoglycan	1019:1045	The cell-wall peptidoglycan contained meso-diaminopimelic acid as the major diagnostic diamino acid.
30431422	8	52	contain	contained	883:891	arg2	phosphatidylglycerol					938:957	phosphatidylglycerol	938:957	phosphatidylglycerol	938:957	The polar lipid profile contained the major components diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and two unidentified aminolipids.
30431422	8	52	contain	contained	883:891	arg2	diphosphatidylglycerol					914:935	diphosphatidylglycerol	914:935	diphosphatidylglycerol	914:935	The polar lipid profile contained the major components diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and two unidentified aminolipids.
30431422	8	52	contain	contained	883:891	arg1	profile					875:881	The polar lipid profile	859:881	The polar lipid profile	859:881	The polar lipid profile contained the major components diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and two unidentified aminolipids.
30431422	8	52	contain	contained	883:891	arg2	components					903:912	the major components	893:912	the major components diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and two unidentified aminolipids	893:1016	The polar lipid profile contained the major components diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and two unidentified aminolipids.
30431422	8	52	contain	contained	883:891	arg2	phosphatidylethanolamine					960:983	phosphatidylethanolamine	960:983	phosphatidylethanolamine	960:983	The polar lipid profile contained the major components diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and two unidentified aminolipids.
30431422	2	53	theme	Gram-stain-positive	89:107	arg1	bacterium					157:165	A Gram-stain-positive, facultatively anaerobic, motile and rod-shaped bacterium	87:165	A Gram-stain-positive, facultatively anaerobic, motile and rod-shaped bacterium	87:165	A Gram-stain-positive, facultatively anaerobic, motile and rod-shaped bacterium, designated KNUC7312T, was isolated from salt-accumulated rhizospheric soil in a pepper greenhouse in Miryang city, Republic of Korea.
30431422	3	54	dep	10-45 °C	346:353	arg1	30 °C					365:369	30 °C	365:369	30 °C	365:369	Cell growth of strain KNUC7312T occurred at 10-45 °C (optimum, 30 °C) and pH 7-12 (optimum, pH 7).
30431422	3	54	dep	10-45 °C	346:353	arg1	optimum					356:362	optimum	356:362	optimum	356:362	Cell growth of strain KNUC7312T occurred at 10-45 °C (optimum, 30 °C) and pH 7-12 (optimum, pH 7).
30431422	4	55	dep	optimum	480:486	arg1	%					493:493	0-1 %	489:493	0-1 %	489:493	In addition, this strain was able to tolerate 0-12 % NaCl (w/v) concentration (optimum, 0-1 %).
30431422	6	56	theme	respiratory	731:741	arg1	menaquinone-7					755:767	menaquinone-7	755:767	menaquinone-7 (MK-7)	755:774	The predominant respiratory quinone was menaquinone-7 (MK-7).
30431422	6	56	theme	respiratory	731:741	arg1	quinone					743:749	The predominant respiratory quinone	715:749	The predominant respiratory quinone	715:749	The predominant respiratory quinone was menaquinone-7 (MK-7).
30431422	4	57	theme	0-12 	447:451	arg1	optimum					480:486	optimum	480:486	optimum	480:486	In addition, this strain was able to tolerate 0-12 % NaCl (w/v) concentration (optimum, 0-1 %).
30431422	4	57	theme	0-12 	447:451	arg1	concentration					465:477	0-12 % NaCl (w/v) concentration	447:477	0-12 % NaCl (w/v) concentration (optimum, 0-1 %)	447:494	In addition, this strain was able to tolerate 0-12 % NaCl (w/v) concentration (optimum, 0-1 %).
30431422	2	58	theme	rhizospheric	225:236	arg1	soil					238:241	salt-accumulated rhizospheric soil	208:241	salt-accumulated rhizospheric soil in a pepper greenhouse in Miryang city, Republic of Korea	208:299	A Gram-stain-positive, facultatively anaerobic, motile and rod-shaped bacterium, designated KNUC7312T, was isolated from salt-accumulated rhizospheric soil in a pepper greenhouse in Miryang city, Republic of Korea.
30431422	5	59	theme	gene	537:540	arg1	sequences					542:550	16S rRNA gene sequences	528:550	16S rRNA gene sequences	528:550	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain KNUC7312T clustered together with other species of the genus Bacillus and was most closely related to Bacillus humi DSM 16318T (98.0 %).
30431422	7	60	dep	C15 	821:824	arg1	 0					826:827	 0	826:827	 0	826:827	The major cellular fatty acids were anteiso C15 : 0, iso-C15 : 0 and iso-C14 : 0.
30431422	8	61	theme	major	897:901	arg1	diphosphatidylglycerol					914:935	diphosphatidylglycerol	914:935	diphosphatidylglycerol	914:935	The polar lipid profile contained the major components diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and two unidentified aminolipids.
30431422	8	61	theme	major	897:901	arg1	phosphatidylethanolamine					960:983	phosphatidylethanolamine	960:983	phosphatidylethanolamine	960:983	The polar lipid profile contained the major components diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and two unidentified aminolipids.
30431422	8	61	theme	major	897:901	arg1	components					903:912	the major components	893:912	the major components diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and two unidentified aminolipids	893:1016	The polar lipid profile contained the major components diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and two unidentified aminolipids.
30431422	8	61	theme	major	897:901	arg1	phosphatidylglycerol					938:957	phosphatidylglycerol	938:957	phosphatidylglycerol	938:957	The polar lipid profile contained the major components diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and two unidentified aminolipids.
30431422	8	62	theme	polar	863:867	arg1	profile					875:881	The polar lipid profile	859:881	The polar lipid profile	859:881	The polar lipid profile contained the major components diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and two unidentified aminolipids.
30431422	2	63	theme	salt-accumulated	208:223	arg1	soil					238:241	salt-accumulated rhizospheric soil	208:241	salt-accumulated rhizospheric soil in a pepper greenhouse in Miryang city, Republic of Korea	208:299	A Gram-stain-positive, facultatively anaerobic, motile and rod-shaped bacterium, designated KNUC7312T, was isolated from salt-accumulated rhizospheric soil in a pepper greenhouse in Miryang city, Republic of Korea.
30431422	9	64	theme	meso-diaminopimelic	1057:1075	arg1	acid					1114:1117	the major diagnostic diamino acid	1085:1117	the major diagnostic diamino acid	1085:1117	The cell-wall peptidoglycan contained meso-diaminopimelic acid as the major diagnostic diamino acid.
30431422	9	64	theme	meso-diaminopimelic	1057:1075	arg1	acid					1077:1080	meso-diaminopimelic acid	1057:1080	meso-diaminopimelic acid	1057:1080	The cell-wall peptidoglycan contained meso-diaminopimelic acid as the major diagnostic diamino acid.
30431422	8	65	dep	components	903:912	arg1	aminolipids					1006:1016	two unidentified aminolipids	989:1016	two unidentified aminolipids	989:1016	The polar lipid profile contained the major components diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and two unidentified aminolipids.
30431422	8	65	dep	components	903:912	arg1	phosphatidylglycerol					938:957	phosphatidylglycerol	938:957	phosphatidylglycerol	938:957	The polar lipid profile contained the major components diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and two unidentified aminolipids.
30431422	8	65	dep	components	903:912	arg1	phosphatidylethanolamine					960:983	phosphatidylethanolamine	960:983	phosphatidylethanolamine	960:983	The polar lipid profile contained the major components diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and two unidentified aminolipids.
30431422	8	65	dep	components	903:912	arg1	components					903:912	the major components	893:912	the major components diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and two unidentified aminolipids	893:1016	The polar lipid profile contained the major components diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and two unidentified aminolipids.
30431422	8	65	dep	components	903:912	arg1	diphosphatidylglycerol					914:935	diphosphatidylglycerol	914:935	diphosphatidylglycerol	914:935	The polar lipid profile contained the major components diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and two unidentified aminolipids.
30431422	10	66	theme	relatedness	1158:1168	arg1	value					1170:1174	a low DNA-DNA relatedness value	1144:1174	a low DNA-DNA relatedness value (47.36 %)	1144:1184	Strain KNUC7312T showed a low DNA-DNA relatedness value (47.36 %) with B. humi DSM 16318T, which supported that this strain represents a novel Bacillusspecies.
30431422	10	66	theme	relatedness	1158:1168	arg1	%					1183:1183	47.36 %	1177:1183	47.36 %	1177:1183	Strain KNUC7312T showed a low DNA-DNA relatedness value (47.36 %) with B. humi DSM 16318T, which supported that this strain represents a novel Bacillusspecies.
30431422	14	67	theme	KCTC	1504:1507	arg1	1.13629T					1522:1529	KCTC 33852T=CGMCC 1.13629T	1504:1529	KCTC 33852T=CGMCC 1.13629T	1504:1529	The type strain is KNUC7312T (KCTC 33852T=CGMCC 1.13629T).
30431422	14	67	theme	KCTC	1504:1507	arg1	KNUC7312T					1493:1501	KNUC7312T	1493:1501	KNUC7312T (KCTC 33852T=CGMCC 1.13629T)	1493:1530	The type strain is KNUC7312T (KCTC 33852T=CGMCC 1.13629T).
30431422	3	68	theme	Cell	302:305	arg1	growth					307:312	Cell growth	302:312	Cell growth of strain KNUC7312T	302:332	Cell growth of strain KNUC7312T occurred at 10-45 °C (optimum, 30 °C) and pH 7-12 (optimum, pH 7).
30431422	6	69	theme	predominant	719:729	arg1	menaquinone-7					755:767	menaquinone-7	755:767	menaquinone-7 (MK-7)	755:774	The predominant respiratory quinone was menaquinone-7 (MK-7).
30431422	6	69	theme	predominant	719:729	arg1	quinone					743:749	The predominant respiratory quinone	715:749	The predominant respiratory quinone	715:749	The predominant respiratory quinone was menaquinone-7 (MK-7).
30431422	14	70	theme	33852T=CGMCC	1509:1520	arg1	1.13629T					1522:1529	KCTC 33852T=CGMCC 1.13629T	1504:1529	KCTC 33852T=CGMCC 1.13629T	1504:1529	The type strain is KNUC7312T (KCTC 33852T=CGMCC 1.13629T).
30431422	14	70	theme	33852T=CGMCC	1509:1520	arg1	KNUC7312T					1493:1501	KNUC7312T	1493:1501	KNUC7312T (KCTC 33852T=CGMCC 1.13629T)	1493:1530	The type strain is KNUC7312T (KCTC 33852T=CGMCC 1.13629T).
30431422	11	71	theme	novel	1380:1384	arg1	species					1386:1392	a novel species	1378:1392	a novel species within the genera Bacillus	1378:1419	On the basis of phenotypic, chemotaxonomic and phylogenetic evidence, strain KNUC7312T represents a novel species within the genera Bacillus.
30431422	7	72	theme	cellular	787:794	arg1	acids					802:806	The major cellular fatty acids	777:806	The major cellular fatty acids	777:806	The major cellular fatty acids were anteiso C15 : 0, iso-C15 : 0 and iso-C14 : 0.
30431422	7	72	theme	cellular	787:794	arg1	C15 					821:824	C15 	821:824	C15 	821:824	The major cellular fatty acids were anteiso C15 : 0, iso-C15 : 0 and iso-C14 : 0.
30431422	11	73	theme	phylogenetic	1327:1338	arg1	evidence					1340:1347	phenotypic, chemotaxonomic and phylogenetic evidence	1296:1347	phenotypic, chemotaxonomic and phylogenetic evidence	1296:1347	On the basis of phenotypic, chemotaxonomic and phylogenetic evidence, strain KNUC7312T represents a novel species within the genera Bacillus.
30431422	7	74	theme	fatty	796:800	arg1	acids					802:806	The major cellular fatty acids	777:806	The major cellular fatty acids	777:806	The major cellular fatty acids were anteiso C15 : 0, iso-C15 : 0 and iso-C14 : 0.
30431422	7	74	theme	fatty	796:800	arg1	C15 					821:824	C15 	821:824	C15 	821:824	The major cellular fatty acids were anteiso C15 : 0, iso-C15 : 0 and iso-C14 : 0.
30431422	2	75	theme	motile	135:140	arg1	bacterium					157:165	A Gram-stain-positive, facultatively anaerobic, motile and rod-shaped bacterium	87:165	A Gram-stain-positive, facultatively anaerobic, motile and rod-shaped bacterium	87:165	A Gram-stain-positive, facultatively anaerobic, motile and rod-shaped bacterium, designated KNUC7312T, was isolated from salt-accumulated rhizospheric soil in a pepper greenhouse in Miryang city, Republic of Korea.
30431422	10	76	theme	novel	1257:1261	arg1	Bacillusspecies					1263:1277	a novel Bacillusspecies	1255:1277	a novel Bacillusspecies	1255:1277	Strain KNUC7312T showed a low DNA-DNA relatedness value (47.36 %) with B. humi DSM 16318T, which supported that this strain represents a novel Bacillusspecies.
29352002	4	0	theme	wild-type	681:689	arg1	mice					701:704	wild-type recipient mice	681:704	wild-type recipient mice	681:704	Importantly, transfer of the microbiota from LKB1ΔIEC mice was sufficient to confer increased susceptibility to DSS-induced colitis in wild-type recipient mice.
29352002	8	1	theme	IL-18	1324:1328	arg1	expression					1330:1339	IL-18 expression	1324:1339	IL-18 expression	1324:1339	Taken together, our study reveals an important function of LKB1 in IECs for suppressing colitogenic microbiota by IL-18 expression.
29352002	3	2	from	susceptibility	389:402	arg1	composition					482:492	the composition	478:492	the composition of the microbial population in the mouse intestine	478:543	IEC-specific deletion of LKB1 (LKB1ΔIEC) resulted in an increased susceptibility to dextran sodium sulfate (DSS)-induced colitis and a definitive shift in the composition of the microbial population in the mouse intestine.
29352002	3	3	from	population	511:520	arg1	intestine					535:543	the mouse intestine	525:543	the mouse intestine	525:543	IEC-specific deletion of LKB1 (LKB1ΔIEC) resulted in an increased susceptibility to dextran sodium sulfate (DSS)-induced colitis and a definitive shift in the composition of the microbial population in the mouse intestine.
29352002	3	4	theme	LKB1	348:351	arg1	deletion					336:343	IEC-specific deletion	323:343	IEC-specific deletion of LKB1 (LKB1ΔIEC)	323:362	IEC-specific deletion of LKB1 (LKB1ΔIEC) resulted in an increased susceptibility to dextran sodium sulfate (DSS)-induced colitis and a definitive shift in the composition of the microbial population in the mouse intestine.
29352002	7	5	theme	genera	1127:1132	arg1	outgrowth					1096:1104	the outgrowth	1092:1104	the outgrowth of several bacterial genera	1092:1132	Administration of exogenous IL-18 restored the expression of antimicrobial peptides, corrected the outgrowth of several bacterial genera, and rescued the LKB1ΔIEC mice from increased sensitivity to DSS challenge.
29352002	3	6	theme	population	511:520	arg1	composition					482:492	the composition	478:492	the composition of the microbial population in the mouse intestine	478:543	IEC-specific deletion of LKB1 (LKB1ΔIEC) resulted in an increased susceptibility to dextran sodium sulfate (DSS)-induced colitis and a definitive shift in the composition of the microbial population in the mouse intestine.
29352002	2	7	link	-derived	252:259	arg1	B1					274:275	intestinal epithelial cell (IEC)-derived liver kinase B1	220:275	intestinal epithelial cell (IEC)-derived liver kinase B1 (LKB1)	220:282	In this study we report a novel role of intestinal epithelial cell (IEC)-derived liver kinase B1 (LKB1) in suppressing colitogenic microbiota.
29352002	2	7	link	-derived	252:259	arg1	LKB1					278:281	LKB1	278:281	LKB1	278:281	In this study we report a novel role of intestinal epithelial cell (IEC)-derived liver kinase B1 (LKB1) in suppressing colitogenic microbiota.
29352002	3	8	from	composition	482:492	arg1	intestine					535:543	the mouse intestine	525:543	the mouse intestine	525:543	IEC-specific deletion of LKB1 (LKB1ΔIEC) resulted in an increased susceptibility to dextran sodium sulfate (DSS)-induced colitis and a definitive shift in the composition of the microbial population in the mouse intestine.
29352002	2	9	theme	B1	274:275	arg1	role					212:215	a novel role	204:215	a novel role of intestinal epithelial cell (IEC)-derived liver kinase B1 (LKB1) in suppressing colitogenic microbiota	204:320	In this study we report a novel role of intestinal epithelial cell (IEC)-derived liver kinase B1 (LKB1) in suppressing colitogenic microbiota.
29352002	3	10	theme	definitive	458:467	arg1	shift					469:473	a definitive shift	456:473	a definitive shift in the composition of the microbial population in the mouse intestine	456:543	IEC-specific deletion of LKB1 (LKB1ΔIEC) resulted in an increased susceptibility to dextran sodium sulfate (DSS)-induced colitis and a definitive shift in the composition of the microbial population in the mouse intestine.
29352002	7	11	theme	several	1109:1115	arg1	genera					1127:1132	several bacterial genera	1109:1132	several bacterial genera	1109:1132	Administration of exogenous IL-18 restored the expression of antimicrobial peptides, corrected the outgrowth of several bacterial genera, and rescued the LKB1ΔIEC mice from increased sensitivity to DSS challenge.
29352002	2	12	theme	kinase	267:272	arg1	B1					274:275	intestinal epithelial cell (IEC)-derived liver kinase B1	220:275	intestinal epithelial cell (IEC)-derived liver kinase B1 (LKB1)	220:282	In this study we report a novel role of intestinal epithelial cell (IEC)-derived liver kinase B1 (LKB1) in suppressing colitogenic microbiota.
29352002	2	12	theme	kinase	267:272	arg1	LKB1					278:281	LKB1	278:281	LKB1	278:281	In this study we report a novel role of intestinal epithelial cell (IEC)-derived liver kinase B1 (LKB1) in suppressing colitogenic microbiota.
29352002	4	13	from	susceptibility	640:653	arg1	mice					701:704	wild-type recipient mice	681:704	wild-type recipient mice	681:704	Importantly, transfer of the microbiota from LKB1ΔIEC mice was sufficient to confer increased susceptibility to DSS-induced colitis in wild-type recipient mice.
29352002	4	14	theme	increased	630:638	arg1	susceptibility					640:653	increased susceptibility	630:653	increased susceptibility to DSS-induced colitis in wild-type recipient mice	630:704	Importantly, transfer of the microbiota from LKB1ΔIEC mice was sufficient to confer increased susceptibility to DSS-induced colitis in wild-type recipient mice.
29352002	6	15	from	deficiency	884:893	arg1	epithelium					913:922	the intestinal epithelium	898:922	the intestinal epithelium	898:922	In addition, LKB1 deficiency in the intestinal epithelium reduced the production of IL-18 and antimicrobial peptides in the colon.
29352002	6	16	theme	LKB1	879:882	arg1	deficiency					884:893	LKB1 deficiency	879:893	LKB1 deficiency in the intestinal epithelium	879:922	In addition, LKB1 deficiency in the intestinal epithelium reduced the production of IL-18 and antimicrobial peptides in the colon.
29352002	5	17	theme	intestinal	763:772	arg1	cells					785:789	intestinal epithelial cells	763:789	intestinal epithelial cells	763:789	Collectively, the data indicate that LKB1 deficiency in intestinal epithelial cells nurtures the outgrowth of colitogenic bacteria in the commensal community.
29352002	1	18	theme	immune	96:101	arg1	function					111:118	the immune barrier function	92:118	the immune barrier function of the intestinal epithelium	92:147	Dysregulation of the immune barrier function of the intestinal epithelium can often result in dysbiosis.
29352002	0	19	theme	Epithelial	11:20	arg1	LKB1					35:38	Intestinal Epithelial Cell-Derived LKB1	0:38	Intestinal Epithelial Cell-Derived LKB1	0:38	Intestinal Epithelial Cell-Derived LKB1 Suppresses Colitogenic Microbiota.
29352002	5	20	from	outgrowth	804:812	arg1	community					855:863	the commensal community	841:863	the commensal community	841:863	Collectively, the data indicate that LKB1 deficiency in intestinal epithelial cells nurtures the outgrowth of colitogenic bacteria in the commensal community.
29352002	1	21	theme	barrier	103:109	arg1	function					111:118	the immune barrier function	92:118	the immune barrier function of the intestinal epithelium	92:147	Dysregulation of the immune barrier function of the intestinal epithelium can often result in dysbiosis.
29352002	0	22	theme	Intestinal	0:9	arg1	LKB1					35:38	Intestinal Epithelial Cell-Derived LKB1	0:38	Intestinal Epithelial Cell-Derived LKB1	0:38	Intestinal Epithelial Cell-Derived LKB1 Suppresses Colitogenic Microbiota.
29352002	8	23	theme	important	1247:1255	arg1	function					1257:1264	an important function	1244:1264	an important function of LKB1 in IECs for suppressing colitogenic microbiota by IL-18 expression	1244:1339	Taken together, our study reveals an important function of LKB1 in IECs for suppressing colitogenic microbiota by IL-18 expression.
29352002	1	24	theme	function	111:118	arg1	Dysregulation					75:87	Dysregulation	75:87	Dysregulation of the immune barrier function of the intestinal epithelium	75:147	Dysregulation of the immune barrier function of the intestinal epithelium can often result in dysbiosis.
29352002	7	25	theme	increased	1170:1178	arg1	sensitivity					1180:1190	increased sensitivity	1170:1190	increased sensitivity to DSS challenge	1170:1207	Administration of exogenous IL-18 restored the expression of antimicrobial peptides, corrected the outgrowth of several bacterial genera, and rescued the LKB1ΔIEC mice from increased sensitivity to DSS challenge.
29352002	6	26	theme	peptides	974:981	arg1	production					936:945	the production	932:945	the production of IL-18 and antimicrobial peptides in the colon	932:994	In addition, LKB1 deficiency in the intestinal epithelium reduced the production of IL-18 and antimicrobial peptides in the colon.
29352002	3	27	from	shift	469:473	arg1	composition					482:492	the composition	478:492	the composition of the microbial population in the mouse intestine	478:543	IEC-specific deletion of LKB1 (LKB1ΔIEC) resulted in an increased susceptibility to dextran sodium sulfate (DSS)-induced colitis and a definitive shift in the composition of the microbial population in the mouse intestine.
29352002	3	28	theme	-induced	435:442	arg1	colitis					444:450	dextran sodium sulfate (DSS)-induced colitis	407:450	dextran sodium sulfate (DSS)-induced colitis	407:450	IEC-specific deletion of LKB1 (LKB1ΔIEC) resulted in an increased susceptibility to dextran sodium sulfate (DSS)-induced colitis and a definitive shift in the composition of the microbial population in the mouse intestine.
29352002	0	29	theme	Cell-Derived	22:33	arg1	LKB1					35:38	Intestinal Epithelial Cell-Derived LKB1	0:38	Intestinal Epithelial Cell-Derived LKB1	0:38	Intestinal Epithelial Cell-Derived LKB1 Suppresses Colitogenic Microbiota.
29352002	6	30	theme	antimicrobial	960:972	arg1	peptides					974:981	antimicrobial peptides	960:981	antimicrobial peptides	960:981	In addition, LKB1 deficiency in the intestinal epithelium reduced the production of IL-18 and antimicrobial peptides in the colon.
29352002	3	31	theme	mouse	529:533	arg1	intestine					535:543	the mouse intestine	525:543	the mouse intestine	525:543	IEC-specific deletion of LKB1 (LKB1ΔIEC) resulted in an increased susceptibility to dextran sodium sulfate (DSS)-induced colitis and a definitive shift in the composition of the microbial population in the mouse intestine.
29352002	8	32	theme	LKB1	1269:1272	arg1	function					1257:1264	an important function	1244:1264	an important function of LKB1 in IECs for suppressing colitogenic microbiota by IL-18 expression	1244:1339	Taken together, our study reveals an important function of LKB1 in IECs for suppressing colitogenic microbiota by IL-18 expression.
29352002	0	33	theme	Colitogenic	51:61	arg1	Microbiota					63:72	Colitogenic Microbiota	51:72	Colitogenic Microbiota	51:72	Intestinal Epithelial Cell-Derived LKB1 Suppresses Colitogenic Microbiota.
29352002	4	34	theme	recipient	691:699	arg1	mice					701:704	wild-type recipient mice	681:704	wild-type recipient mice	681:704	Importantly, transfer of the microbiota from LKB1ΔIEC mice was sufficient to confer increased susceptibility to DSS-induced colitis in wild-type recipient mice.
29352002	7	35	theme	peptides	1072:1079	arg1	expression					1044:1053	the expression	1040:1053	the expression of antimicrobial peptides	1040:1079	Administration of exogenous IL-18 restored the expression of antimicrobial peptides, corrected the outgrowth of several bacterial genera, and rescued the LKB1ΔIEC mice from increased sensitivity to DSS challenge.
29352002	3	36	theme	microbial	501:509	arg1	population					511:520	the microbial population	497:520	the microbial population in the mouse intestine	497:543	IEC-specific deletion of LKB1 (LKB1ΔIEC) resulted in an increased susceptibility to dextran sodium sulfate (DSS)-induced colitis and a definitive shift in the composition of the microbial population in the mouse intestine.
29352002	2	37	theme	cell	242:245	arg1	B1					274:275	intestinal epithelial cell (IEC)-derived liver kinase B1	220:275	intestinal epithelial cell (IEC)-derived liver kinase B1 (LKB1)	220:282	In this study we report a novel role of intestinal epithelial cell (IEC)-derived liver kinase B1 (LKB1) in suppressing colitogenic microbiota.
29352002	2	37	theme	cell	242:245	arg1	LKB1					278:281	LKB1	278:281	LKB1	278:281	In this study we report a novel role of intestinal epithelial cell (IEC)-derived liver kinase B1 (LKB1) in suppressing colitogenic microbiota.
29352002	4	38	from	mice	600:603	arg1	transfer					559:566	transfer	559:566	transfer of the microbiota from LKB1ΔIEC mice	559:603	Importantly, transfer of the microbiota from LKB1ΔIEC mice was sufficient to confer increased susceptibility to DSS-induced colitis in wild-type recipient mice.
29352002	5	39	theme	epithelial	774:783	arg1	cells					785:789	intestinal epithelial cells	763:789	intestinal epithelial cells	763:789	Collectively, the data indicate that LKB1 deficiency in intestinal epithelial cells nurtures the outgrowth of colitogenic bacteria in the commensal community.
29352002	6	40	theme	IL-18	950:954	arg1	production					936:945	the production	932:945	the production of IL-18 and antimicrobial peptides in the colon	932:994	In addition, LKB1 deficiency in the intestinal epithelium reduced the production of IL-18 and antimicrobial peptides in the colon.
29352002	2	41	theme	epithelial	231:240	arg1	IEC					248:250	IEC	248:250	IEC	248:250	In this study we report a novel role of intestinal epithelial cell (IEC)-derived liver kinase B1 (LKB1) in suppressing colitogenic microbiota.
29352002	2	41	theme	epithelial	231:240	arg1	cell					242:245	intestinal epithelial cell	220:245	intestinal epithelial cell (IEC)-derived liver kinase B1 (LKB1)	220:282	In this study we report a novel role of intestinal epithelial cell (IEC)-derived liver kinase B1 (LKB1) in suppressing colitogenic microbiota.
29352002	7	42	theme	IL-18	1025:1029	arg1	Administration					997:1010	Administration	997:1010	Administration of exogenous IL-18	997:1029	Administration of exogenous IL-18 restored the expression of antimicrobial peptides, corrected the outgrowth of several bacterial genera, and rescued the LKB1ΔIEC mice from increased sensitivity to DSS challenge.
29352002	5	43	theme	commensal	845:853	arg1	community					855:863	the commensal community	841:863	the commensal community	841:863	Collectively, the data indicate that LKB1 deficiency in intestinal epithelial cells nurtures the outgrowth of colitogenic bacteria in the commensal community.
29352002	8	44	theme	colitogenic	1298:1308	arg1	microbiota					1310:1319	colitogenic microbiota	1298:1319	colitogenic microbiota	1298:1319	Taken together, our study reveals an important function of LKB1 in IECs for suppressing colitogenic microbiota by IL-18 expression.
29352002	6	45	from	production	936:945	arg1	colon					990:994	the colon	986:994	the colon	986:994	In addition, LKB1 deficiency in the intestinal epithelium reduced the production of IL-18 and antimicrobial peptides in the colon.
29352002	5	46	theme	LKB1	744:747	arg1	deficiency					749:758	LKB1 deficiency	744:758	LKB1 deficiency in intestinal epithelial cells	744:789	Collectively, the data indicate that LKB1 deficiency in intestinal epithelial cells nurtures the outgrowth of colitogenic bacteria in the commensal community.
29352002	2	47	theme	intestinal	220:229	arg1	IEC					248:250	IEC	248:250	IEC	248:250	In this study we report a novel role of intestinal epithelial cell (IEC)-derived liver kinase B1 (LKB1) in suppressing colitogenic microbiota.
29352002	2	47	theme	intestinal	220:229	arg1	cell					242:245	intestinal epithelial cell	220:245	intestinal epithelial cell (IEC)-derived liver kinase B1 (LKB1)	220:282	In this study we report a novel role of intestinal epithelial cell (IEC)-derived liver kinase B1 (LKB1) in suppressing colitogenic microbiota.
29352002	8	48	from	function	1257:1264	arg1	IECs					1277:1280	IECs	1277:1280	IECs	1277:1280	Taken together, our study reveals an important function of LKB1 in IECs for suppressing colitogenic microbiota by IL-18 expression.
29352002	1	49	theme	intestinal	127:136	arg1	epithelium					138:147	the intestinal epithelium	123:147	the intestinal epithelium	123:147	Dysregulation of the immune barrier function of the intestinal epithelium can often result in dysbiosis.
29352002	3	50	theme	increased	379:387	arg1	susceptibility					389:402	an increased susceptibility	376:402	an increased susceptibility to dextran sodium sulfate (DSS)-induced colitis	376:450	IEC-specific deletion of LKB1 (LKB1ΔIEC) resulted in an increased susceptibility to dextran sodium sulfate (DSS)-induced colitis and a definitive shift in the composition of the microbial population in the mouse intestine.
29352002	7	51	theme	antimicrobial	1058:1070	arg1	peptides					1072:1079	antimicrobial peptides	1058:1079	antimicrobial peptides	1058:1079	Administration of exogenous IL-18 restored the expression of antimicrobial peptides, corrected the outgrowth of several bacterial genera, and rescued the LKB1ΔIEC mice from increased sensitivity to DSS challenge.
29352002	5	52	from	deficiency	749:758	arg1	cells					785:789	intestinal epithelial cells	763:789	intestinal epithelial cells	763:789	Collectively, the data indicate that LKB1 deficiency in intestinal epithelial cells nurtures the outgrowth of colitogenic bacteria in the commensal community.
29352002	7	53	theme	bacterial	1117:1125	arg1	genera					1127:1132	several bacterial genera	1109:1132	several bacterial genera	1109:1132	Administration of exogenous IL-18 restored the expression of antimicrobial peptides, corrected the outgrowth of several bacterial genera, and rescued the LKB1ΔIEC mice from increased sensitivity to DSS challenge.
29352002	1	54	theme	epithelium	138:147	arg1	function					111:118	the immune barrier function	92:118	the immune barrier function of the intestinal epithelium	92:147	Dysregulation of the immune barrier function of the intestinal epithelium can often result in dysbiosis.
29352002	7	55	theme	LKB1ΔIEC	1151:1158	arg1	mice					1160:1163	the LKB1ΔIEC mice	1147:1163	the LKB1ΔIEC mice	1147:1163	Administration of exogenous IL-18 restored the expression of antimicrobial peptides, corrected the outgrowth of several bacterial genera, and rescued the LKB1ΔIEC mice from increased sensitivity to DSS challenge.
29352002	2	56	theme	novel	206:210	arg1	role					212:215	a novel role	204:215	a novel role of intestinal epithelial cell (IEC)-derived liver kinase B1 (LKB1) in suppressing colitogenic microbiota	204:320	In this study we report a novel role of intestinal epithelial cell (IEC)-derived liver kinase B1 (LKB1) in suppressing colitogenic microbiota.
29352002	2	57	theme	-derived	252:259	arg1	B1					274:275	intestinal epithelial cell (IEC)-derived liver kinase B1	220:275	intestinal epithelial cell (IEC)-derived liver kinase B1 (LKB1)	220:282	In this study we report a novel role of intestinal epithelial cell (IEC)-derived liver kinase B1 (LKB1) in suppressing colitogenic microbiota.
29352002	2	57	theme	-derived	252:259	arg1	LKB1					278:281	LKB1	278:281	LKB1	278:281	In this study we report a novel role of intestinal epithelial cell (IEC)-derived liver kinase B1 (LKB1) in suppressing colitogenic microbiota.
29352002	7	58	theme	exogenous	1015:1023	arg1	IL-18					1025:1029	exogenous IL-18	1015:1029	exogenous IL-18	1015:1029	Administration of exogenous IL-18 restored the expression of antimicrobial peptides, corrected the outgrowth of several bacterial genera, and rescued the LKB1ΔIEC mice from increased sensitivity to DSS challenge.
29352002	6	59	theme	intestinal	902:911	arg1	epithelium					913:922	the intestinal epithelium	898:922	the intestinal epithelium	898:922	In addition, LKB1 deficiency in the intestinal epithelium reduced the production of IL-18 and antimicrobial peptides in the colon.
29352002	4	60	theme	microbiota	575:584	arg1	transfer					559:566	transfer	559:566	transfer of the microbiota from LKB1ΔIEC mice	559:603	Importantly, transfer of the microbiota from LKB1ΔIEC mice was sufficient to confer increased susceptibility to DSS-induced colitis in wild-type recipient mice.
29352002	2	61	theme	liver	261:265	arg1	B1					274:275	intestinal epithelial cell (IEC)-derived liver kinase B1	220:275	intestinal epithelial cell (IEC)-derived liver kinase B1 (LKB1)	220:282	In this study we report a novel role of intestinal epithelial cell (IEC)-derived liver kinase B1 (LKB1) in suppressing colitogenic microbiota.
29352002	2	61	theme	liver	261:265	arg1	LKB1					278:281	LKB1	278:281	LKB1	278:281	In this study we report a novel role of intestinal epithelial cell (IEC)-derived liver kinase B1 (LKB1) in suppressing colitogenic microbiota.
29352002	2	62	theme	colitogenic	299:309	arg1	microbiota					311:320	colitogenic microbiota	299:320	colitogenic microbiota	299:320	In this study we report a novel role of intestinal epithelial cell (IEC)-derived liver kinase B1 (LKB1) in suppressing colitogenic microbiota.
29352002	3	63	from	intestine	535:543	arg1	composition					482:492	the composition	478:492	the composition of the microbial population in the mouse intestine	478:543	IEC-specific deletion of LKB1 (LKB1ΔIEC) resulted in an increased susceptibility to dextran sodium sulfate (DSS)-induced colitis and a definitive shift in the composition of the microbial population in the mouse intestine.
29352002	5	64	theme	bacteria	829:836	arg1	outgrowth					804:812	the outgrowth	800:812	the outgrowth of colitogenic bacteria in the commensal community	800:863	Collectively, the data indicate that LKB1 deficiency in intestinal epithelial cells nurtures the outgrowth of colitogenic bacteria in the commensal community.
29352002	4	65	theme	LKB1ΔIEC	591:598	arg1	mice					600:603	LKB1ΔIEC mice	591:603	LKB1ΔIEC mice	591:603	Importantly, transfer of the microbiota from LKB1ΔIEC mice was sufficient to confer increased susceptibility to DSS-induced colitis in wild-type recipient mice.
29352002	3	66	theme	IEC-specific	323:334	arg1	deletion					336:343	IEC-specific deletion	323:343	IEC-specific deletion of LKB1 (LKB1ΔIEC)	323:362	IEC-specific deletion of LKB1 (LKB1ΔIEC) resulted in an increased susceptibility to dextran sodium sulfate (DSS)-induced colitis and a definitive shift in the composition of the microbial population in the mouse intestine.
29352002	5	67	theme	colitogenic	817:827	arg1	bacteria					829:836	colitogenic bacteria	817:836	colitogenic bacteria	817:836	Collectively, the data indicate that LKB1 deficiency in intestinal epithelial cells nurtures the outgrowth of colitogenic bacteria in the commensal community.
29352002	4	68	theme	DSS-induced	658:668	arg1	colitis					670:676	DSS-induced colitis	658:676	DSS-induced colitis	658:676	Importantly, transfer of the microbiota from LKB1ΔIEC mice was sufficient to confer increased susceptibility to DSS-induced colitis in wild-type recipient mice.
29352002	7	69	theme	DSS	1195:1197	arg1	challenge					1199:1207	DSS challenge	1195:1207	DSS challenge	1195:1207	Administration of exogenous IL-18 restored the expression of antimicrobial peptides, corrected the outgrowth of several bacterial genera, and rescued the LKB1ΔIEC mice from increased sensitivity to DSS challenge.
30120787	2	0	theme	forced	447:452	arg1	model					463:467	a forced swimming model	445:467	a forced swimming model	445:467	In this study, crude macamide extract (CME) and purified macamide extract (PME) were prepared by enzyme-assisted extraction and macroporous resin separation, and the anti-fatigue effects of CME and PME were evaluated in a forced swimming model.
30120787	4	1	theme	glycogen	788:795	arg1	levels					747:752	increase levels	738:752	increase levels of liver glycogen (LG) and muscle glycogen (MG)	738:800	Based on the results of a weight-loaded forced swimming test, compared with a control group, CME and and PME groups could prolong exhaustive swimming time, increase levels of liver glycogen (LG) and muscle glycogen (MG), accelerate fatty acid oxidation in serum to provide energy, eliminate the accumulation of blood lactic acid (BLA) and blood urea nitrogen (BUN), and decrease the serum biomarkers for muscle damage, such as lactate dehydrogenase (LDH) and creatine kinase (CK).
30120787	4	2	theme	urea	927:930	arg1	BUN					942:944	BUN	942:944	BUN	942:944	Based on the results of a weight-loaded forced swimming test, compared with a control group, CME and and PME groups could prolong exhaustive swimming time, increase levels of liver glycogen (LG) and muscle glycogen (MG), accelerate fatty acid oxidation in serum to provide energy, eliminate the accumulation of blood lactic acid (BLA) and blood urea nitrogen (BUN), and decrease the serum biomarkers for muscle damage, such as lactate dehydrogenase (LDH) and creatine kinase (CK).
30120787	4	2	theme	urea	927:930	arg1	nitrogen					932:939	blood urea nitrogen	921:939	blood urea nitrogen (BUN)	921:945	Based on the results of a weight-loaded forced swimming test, compared with a control group, CME and and PME groups could prolong exhaustive swimming time, increase levels of liver glycogen (LG) and muscle glycogen (MG), accelerate fatty acid oxidation in serum to provide energy, eliminate the accumulation of blood lactic acid (BLA) and blood urea nitrogen (BUN), and decrease the serum biomarkers for muscle damage, such as lactate dehydrogenase (LDH) and creatine kinase (CK).
30120787	3	3	contain	contain	549:555	arg1	CME					530:532	CME	530:532	CME	530:532	RESULTS The composition analysis results revealed that both CME and PME mainly contain eight kinds of macamide.
30120787	3	3	contain	contain	549:555	arg2	kinds					563:567	eight kinds	557:567	eight kinds of macamide	557:579	RESULTS The composition analysis results revealed that both CME and PME mainly contain eight kinds of macamide.
30120787	3	3	contain	contain	549:555	arg1	PME					538:540	PME	538:540	PME	538:540	RESULTS The composition analysis results revealed that both CME and PME mainly contain eight kinds of macamide.
30120787	6	4	theme	macamide	1213:1220	arg1	extracts					1222:1229	CONCLUSION Two macamide extracts	1198:1229	CONCLUSION Two macamide extracts	1198:1229	CONCLUSION Two macamide extracts have a beneficial effect on relieving physical fatigue by attenuating the damage of skeletal muscle and myocardium during exercise, and a better effect was observed in the PME group.
30120787	4	5	from	oxidation	825:833	arg1	serum					838:842	serum	838:842	serum to provide energy, eliminate the accumulation of blood lactic acid (BLA) and blood urea nitrogen (BUN), and decrease the serum biomarkers for muscle damage, such as lactate dehydrogenase (LDH) and creatine kinase (CK)	838:1060	Based on the results of a weight-loaded forced swimming test, compared with a control group, CME and and PME groups could prolong exhaustive swimming time, increase levels of liver glycogen (LG) and muscle glycogen (MG), accelerate fatty acid oxidation in serum to provide energy, eliminate the accumulation of blood lactic acid (BLA) and blood urea nitrogen (BUN), and decrease the serum biomarkers for muscle damage, such as lactate dehydrogenase (LDH) and creatine kinase (CK).
30120787	4	6	theme	muscle	781:786	arg1	glycogen					788:795	muscle glycogen	781:795	muscle glycogen (MG)	781:800	Based on the results of a weight-loaded forced swimming test, compared with a control group, CME and and PME groups could prolong exhaustive swimming time, increase levels of liver glycogen (LG) and muscle glycogen (MG), accelerate fatty acid oxidation in serum to provide energy, eliminate the accumulation of blood lactic acid (BLA) and blood urea nitrogen (BUN), and decrease the serum biomarkers for muscle damage, such as lactate dehydrogenase (LDH) and creatine kinase (CK).
30120787	4	6	theme	muscle	781:786	arg1	MG					798:799	MG	798:799	MG	798:799	Based on the results of a weight-loaded forced swimming test, compared with a control group, CME and and PME groups could prolong exhaustive swimming time, increase levels of liver glycogen (LG) and muscle glycogen (MG), accelerate fatty acid oxidation in serum to provide energy, eliminate the accumulation of blood lactic acid (BLA) and blood urea nitrogen (BUN), and decrease the serum biomarkers for muscle damage, such as lactate dehydrogenase (LDH) and creatine kinase (CK).
30120787	6	7	theme	physical	1269:1276	arg1	fatigue					1278:1284	physical fatigue	1269:1284	physical fatigue	1269:1284	CONCLUSION Two macamide extracts have a beneficial effect on relieving physical fatigue by attenuating the damage of skeletal muscle and myocardium during exercise, and a better effect was observed in the PME group.
30120787	4	8	dep	serum	838:842	arg1	provide					847:853	provide	847:853	provide	847:853	Based on the results of a weight-loaded forced swimming test, compared with a control group, CME and and PME groups could prolong exhaustive swimming time, increase levels of liver glycogen (LG) and muscle glycogen (MG), accelerate fatty acid oxidation in serum to provide energy, eliminate the accumulation of blood lactic acid (BLA) and blood urea nitrogen (BUN), and decrease the serum biomarkers for muscle damage, such as lactate dehydrogenase (LDH) and creatine kinase (CK).
30120787	4	8	dep	serum	838:842	arg1	eliminate					863:871	eliminate	863:871	eliminate the accumulation of blood lactic acid (BLA) and blood urea nitrogen (BUN)	863:945	Based on the results of a weight-loaded forced swimming test, compared with a control group, CME and and PME groups could prolong exhaustive swimming time, increase levels of liver glycogen (LG) and muscle glycogen (MG), accelerate fatty acid oxidation in serum to provide energy, eliminate the accumulation of blood lactic acid (BLA) and blood urea nitrogen (BUN), and decrease the serum biomarkers for muscle damage, such as lactate dehydrogenase (LDH) and creatine kinase (CK).
30120787	4	8	dep	serum	838:842	arg1	decrease					952:959	decrease	952:959	decrease the serum biomarkers for muscle damage, such as lactate dehydrogenase (LDH) and creatine kinase (CK)	952:1060	Based on the results of a weight-loaded forced swimming test, compared with a control group, CME and and PME groups could prolong exhaustive swimming time, increase levels of liver glycogen (LG) and muscle glycogen (MG), accelerate fatty acid oxidation in serum to provide energy, eliminate the accumulation of blood lactic acid (BLA) and blood urea nitrogen (BUN), and decrease the serum biomarkers for muscle damage, such as lactate dehydrogenase (LDH) and creatine kinase (CK).
30120787	4	9	theme	swimming	723:730	arg1	time					732:735	exhaustive swimming time	712:735	exhaustive swimming time	712:735	Based on the results of a weight-loaded forced swimming test, compared with a control group, CME and and PME groups could prolong exhaustive swimming time, increase levels of liver glycogen (LG) and muscle glycogen (MG), accelerate fatty acid oxidation in serum to provide energy, eliminate the accumulation of blood lactic acid (BLA) and blood urea nitrogen (BUN), and decrease the serum biomarkers for muscle damage, such as lactate dehydrogenase (LDH) and creatine kinase (CK).
30120787	6	10	theme	CONCLUSION	1198:1207	arg1	extracts					1222:1229	CONCLUSION Two macamide extracts	1198:1229	CONCLUSION Two macamide extracts	1198:1229	CONCLUSION Two macamide extracts have a beneficial effect on relieving physical fatigue by attenuating the damage of skeletal muscle and myocardium during exercise, and a better effect was observed in the PME group.
30120787	6	11	theme	skeletal	1315:1322	arg1	muscle					1324:1329	skeletal muscle	1315:1329	skeletal muscle	1315:1329	CONCLUSION Two macamide extracts have a beneficial effect on relieving physical fatigue by attenuating the damage of skeletal muscle and myocardium during exercise, and a better effect was observed in the PME group.
30120787	6	12	contain	have	1231:1234	arg1	extracts					1222:1229	CONCLUSION Two macamide extracts	1198:1229	CONCLUSION Two macamide extracts	1198:1229	CONCLUSION Two macamide extracts have a beneficial effect on relieving physical fatigue by attenuating the damage of skeletal muscle and myocardium during exercise, and a better effect was observed in the PME group.
30120787	6	12	contain	have	1231:1234	arg2	effect					1249:1254	a beneficial effect	1236:1254	a beneficial effect	1236:1254	CONCLUSION Two macamide extracts have a beneficial effect on relieving physical fatigue by attenuating the damage of skeletal muscle and myocardium during exercise, and a better effect was observed in the PME group.
30120787	4	13	theme	control	660:666	arg1	group					668:672	a control group	658:672	a control group	658:672	Based on the results of a weight-loaded forced swimming test, compared with a control group, CME and and PME groups could prolong exhaustive swimming time, increase levels of liver glycogen (LG) and muscle glycogen (MG), accelerate fatty acid oxidation in serum to provide energy, eliminate the accumulation of blood lactic acid (BLA) and blood urea nitrogen (BUN), and decrease the serum biomarkers for muscle damage, such as lactate dehydrogenase (LDH) and creatine kinase (CK).
30120787	4	14	theme	exhaustive	712:721	arg1	time					732:735	exhaustive swimming time	712:735	exhaustive swimming time	712:735	Based on the results of a weight-loaded forced swimming test, compared with a control group, CME and and PME groups could prolong exhaustive swimming time, increase levels of liver glycogen (LG) and muscle glycogen (MG), accelerate fatty acid oxidation in serum to provide energy, eliminate the accumulation of blood lactic acid (BLA) and blood urea nitrogen (BUN), and decrease the serum biomarkers for muscle damage, such as lactate dehydrogenase (LDH) and creatine kinase (CK).
30120787	2	15	theme	macroporous	353:363	arg1	separation					371:380	macroporous resin separation	353:380	macroporous resin separation	353:380	In this study, crude macamide extract (CME) and purified macamide extract (PME) were prepared by enzyme-assisted extraction and macroporous resin separation, and the anti-fatigue effects of CME and PME were evaluated in a forced swimming model.
30120787	4	16	theme	weight-loaded	608:620	arg1	test					638:641	a weight-loaded forced swimming test	606:641	a weight-loaded forced swimming test	606:641	Based on the results of a weight-loaded forced swimming test, compared with a control group, CME and and PME groups could prolong exhaustive swimming time, increase levels of liver glycogen (LG) and muscle glycogen (MG), accelerate fatty acid oxidation in serum to provide energy, eliminate the accumulation of blood lactic acid (BLA) and blood urea nitrogen (BUN), and decrease the serum biomarkers for muscle damage, such as lactate dehydrogenase (LDH) and creatine kinase (CK).
30120787	4	17	theme	acid	906:909	arg1	accumulation					877:888	the accumulation	873:888	the accumulation of blood lactic acid (BLA) and blood urea nitrogen (BUN)	873:945	Based on the results of a weight-loaded forced swimming test, compared with a control group, CME and and PME groups could prolong exhaustive swimming time, increase levels of liver glycogen (LG) and muscle glycogen (MG), accelerate fatty acid oxidation in serum to provide energy, eliminate the accumulation of blood lactic acid (BLA) and blood urea nitrogen (BUN), and decrease the serum biomarkers for muscle damage, such as lactate dehydrogenase (LDH) and creatine kinase (CK).
30120787	6	18	located	observed	1387:1394	arg2	effect					1376:1381	a better effect	1367:1381	a better effect	1367:1381	CONCLUSION Two macamide extracts have a beneficial effect on relieving physical fatigue by attenuating the damage of skeletal muscle and myocardium during exercise, and a better effect was observed in the PME group.
30120787	6	18	located	observed	1387:1394	arg1	group					1407:1411	the PME group	1399:1411	the PME group	1399:1411	CONCLUSION Two macamide extracts have a beneficial effect on relieving physical fatigue by attenuating the damage of skeletal muscle and myocardium during exercise, and a better effect was observed in the PME group.
30120787	2	19	theme	resin	365:369	arg1	separation					371:380	macroporous resin separation	353:380	macroporous resin separation	353:380	In this study, crude macamide extract (CME) and purified macamide extract (PME) were prepared by enzyme-assisted extraction and macroporous resin separation, and the anti-fatigue effects of CME and PME were evaluated in a forced swimming model.
30120787	0	20	theme	macamide	4:11	arg1	extracts					13:20	Two macamide extracts	0:20	Two macamide extracts	0:20	Two macamide extracts relieve physical fatigue by attenuating muscle damage in mice.
30120787	6	21	theme	myocardium	1335:1344	arg1	damage					1305:1310	the damage	1301:1310	the damage of skeletal muscle and myocardium	1301:1344	CONCLUSION Two macamide extracts have a beneficial effect on relieving physical fatigue by attenuating the damage of skeletal muscle and myocardium during exercise, and a better effect was observed in the PME group.
30120787	5	22	from	damage	1128:1133	arg1	mice					1176:1179	mice	1176:1179	mice	1176:1179	Histological analysis also indicated that CME and PME attenuated damage to skeletal muscle and the myocardium in mice during exercise.
30120787	6	23	theme	better	1369:1374	arg1	effect					1376:1381	a better effect	1367:1381	a better effect	1367:1381	CONCLUSION Two macamide extracts have a beneficial effect on relieving physical fatigue by attenuating the damage of skeletal muscle and myocardium during exercise, and a better effect was observed in the PME group.
30120787	1	24	theme	increasing	168:177	arg1	attention					179:187	increasing attention	168:187	increasing attention	168:187	BACKGROUND Macamides, the main active components contained in maca, have attracted increasing attention due to their various bioactivities.
30120787	2	25	theme	CME	415:417	arg1	effects					404:410	the anti-fatigue effects	387:410	the anti-fatigue effects of CME and PME	387:425	In this study, crude macamide extract (CME) and purified macamide extract (PME) were prepared by enzyme-assisted extraction and macroporous resin separation, and the anti-fatigue effects of CME and PME were evaluated in a forced swimming model.
30120787	1	26	theme	BACKGROUND	85:94	arg1	components					123:132	the main active components	107:132	the main active components contained in maca	107:150	BACKGROUND Macamides, the main active components contained in maca, have attracted increasing attention due to their various bioactivities.
30120787	1	26	theme	BACKGROUND	85:94	arg1	Macamides					96:104	BACKGROUND Macamides	85:104	BACKGROUND Macamides	85:104	BACKGROUND Macamides, the main active components contained in maca, have attracted increasing attention due to their various bioactivities.
30120787	4	27	theme	serum	965:969	arg1	biomarkers					971:980	the serum biomarkers	961:980	the serum biomarkers	961:980	Based on the results of a weight-loaded forced swimming test, compared with a control group, CME and and PME groups could prolong exhaustive swimming time, increase levels of liver glycogen (LG) and muscle glycogen (MG), accelerate fatty acid oxidation in serum to provide energy, eliminate the accumulation of blood lactic acid (BLA) and blood urea nitrogen (BUN), and decrease the serum biomarkers for muscle damage, such as lactate dehydrogenase (LDH) and creatine kinase (CK).
30120787	4	27	theme	serum	965:969	arg1	dehydrogenase					1017:1029	lactate dehydrogenase	1009:1029	lactate dehydrogenase (LDH)	1009:1035	Based on the results of a weight-loaded forced swimming test, compared with a control group, CME and and PME groups could prolong exhaustive swimming time, increase levels of liver glycogen (LG) and muscle glycogen (MG), accelerate fatty acid oxidation in serum to provide energy, eliminate the accumulation of blood lactic acid (BLA) and blood urea nitrogen (BUN), and decrease the serum biomarkers for muscle damage, such as lactate dehydrogenase (LDH) and creatine kinase (CK).
30120787	4	27	theme	serum	965:969	arg1	kinase					1050:1055	creatine kinase	1041:1055	creatine kinase (CK)	1041:1060	Based on the results of a weight-loaded forced swimming test, compared with a control group, CME and and PME groups could prolong exhaustive swimming time, increase levels of liver glycogen (LG) and muscle glycogen (MG), accelerate fatty acid oxidation in serum to provide energy, eliminate the accumulation of blood lactic acid (BLA) and blood urea nitrogen (BUN), and decrease the serum biomarkers for muscle damage, such as lactate dehydrogenase (LDH) and creatine kinase (CK).
30120787	4	28	theme	forced	622:627	arg1	test					638:641	a weight-loaded forced swimming test	606:641	a weight-loaded forced swimming test	606:641	Based on the results of a weight-loaded forced swimming test, compared with a control group, CME and and PME groups could prolong exhaustive swimming time, increase levels of liver glycogen (LG) and muscle glycogen (MG), accelerate fatty acid oxidation in serum to provide energy, eliminate the accumulation of blood lactic acid (BLA) and blood urea nitrogen (BUN), and decrease the serum biomarkers for muscle damage, such as lactate dehydrogenase (LDH) and creatine kinase (CK).
30120787	4	29	theme	blood	921:925	arg1	BUN					942:944	BUN	942:944	BUN	942:944	Based on the results of a weight-loaded forced swimming test, compared with a control group, CME and and PME groups could prolong exhaustive swimming time, increase levels of liver glycogen (LG) and muscle glycogen (MG), accelerate fatty acid oxidation in serum to provide energy, eliminate the accumulation of blood lactic acid (BLA) and blood urea nitrogen (BUN), and decrease the serum biomarkers for muscle damage, such as lactate dehydrogenase (LDH) and creatine kinase (CK).
30120787	4	29	theme	blood	921:925	arg1	nitrogen					932:939	blood urea nitrogen	921:939	blood urea nitrogen (BUN)	921:945	Based on the results of a weight-loaded forced swimming test, compared with a control group, CME and and PME groups could prolong exhaustive swimming time, increase levels of liver glycogen (LG) and muscle glycogen (MG), accelerate fatty acid oxidation in serum to provide energy, eliminate the accumulation of blood lactic acid (BLA) and blood urea nitrogen (BUN), and decrease the serum biomarkers for muscle damage, such as lactate dehydrogenase (LDH) and creatine kinase (CK).
30120787	5	30	from	myocardium	1162:1171	arg1	mice					1176:1179	mice	1176:1179	mice	1176:1179	Histological analysis also indicated that CME and PME attenuated damage to skeletal muscle and the myocardium in mice during exercise.
30120787	4	31	theme	liver	757:761	arg1	LG					773:774	LG	773:774	LG	773:774	Based on the results of a weight-loaded forced swimming test, compared with a control group, CME and and PME groups could prolong exhaustive swimming time, increase levels of liver glycogen (LG) and muscle glycogen (MG), accelerate fatty acid oxidation in serum to provide energy, eliminate the accumulation of blood lactic acid (BLA) and blood urea nitrogen (BUN), and decrease the serum biomarkers for muscle damage, such as lactate dehydrogenase (LDH) and creatine kinase (CK).
30120787	4	31	theme	liver	757:761	arg1	glycogen					763:770	liver glycogen	757:770	liver glycogen (LG)	757:775	Based on the results of a weight-loaded forced swimming test, compared with a control group, CME and and PME groups could prolong exhaustive swimming time, increase levels of liver glycogen (LG) and muscle glycogen (MG), accelerate fatty acid oxidation in serum to provide energy, eliminate the accumulation of blood lactic acid (BLA) and blood urea nitrogen (BUN), and decrease the serum biomarkers for muscle damage, such as lactate dehydrogenase (LDH) and creatine kinase (CK).
30120787	2	32	theme	purified	273:280	arg1	PME					300:302	PME	300:302	PME	300:302	In this study, crude macamide extract (CME) and purified macamide extract (PME) were prepared by enzyme-assisted extraction and macroporous resin separation, and the anti-fatigue effects of CME and PME were evaluated in a forced swimming model.
30120787	2	32	theme	purified	273:280	arg1	extract					291:297	purified macamide extract	273:297	purified macamide extract (PME)	273:303	In this study, crude macamide extract (CME) and purified macamide extract (PME) were prepared by enzyme-assisted extraction and macroporous resin separation, and the anti-fatigue effects of CME and PME were evaluated in a forced swimming model.
30120787	2	33	theme	anti-fatigue	391:402	arg1	effects					404:410	the anti-fatigue effects	387:410	the anti-fatigue effects of CME and PME	387:425	In this study, crude macamide extract (CME) and purified macamide extract (PME) were prepared by enzyme-assisted extraction and macroporous resin separation, and the anti-fatigue effects of CME and PME were evaluated in a forced swimming model.
30120787	2	34	theme	macamide	282:289	arg1	PME					300:302	PME	300:302	PME	300:302	In this study, crude macamide extract (CME) and purified macamide extract (PME) were prepared by enzyme-assisted extraction and macroporous resin separation, and the anti-fatigue effects of CME and PME were evaluated in a forced swimming model.
30120787	2	34	theme	macamide	282:289	arg1	extract					291:297	purified macamide extract	273:297	purified macamide extract (PME)	273:303	In this study, crude macamide extract (CME) and purified macamide extract (PME) were prepared by enzyme-assisted extraction and macroporous resin separation, and the anti-fatigue effects of CME and PME were evaluated in a forced swimming model.
30120787	4	35	theme	acid	820:823	arg1	oxidation					825:833	fatty acid oxidation	814:833	fatty acid oxidation in serum to provide energy, eliminate the accumulation of blood lactic acid (BLA) and blood urea nitrogen (BUN), and decrease the serum biomarkers for muscle damage, such as lactate dehydrogenase (LDH) and creatine kinase (CK)	814:1060	Based on the results of a weight-loaded forced swimming test, compared with a control group, CME and and PME groups could prolong exhaustive swimming time, increase levels of liver glycogen (LG) and muscle glycogen (MG), accelerate fatty acid oxidation in serum to provide energy, eliminate the accumulation of blood lactic acid (BLA) and blood urea nitrogen (BUN), and decrease the serum biomarkers for muscle damage, such as lactate dehydrogenase (LDH) and creatine kinase (CK).
30120787	5	36	theme	skeletal	1138:1145	arg1	muscle					1147:1152	skeletal muscle	1138:1152	skeletal muscle	1138:1152	Histological analysis also indicated that CME and PME attenuated damage to skeletal muscle and the myocardium in mice during exercise.
30120787	4	37	theme	lactic	899:904	arg1	acid					906:909	blood lactic acid	893:909	blood lactic acid (BLA)	893:915	Based on the results of a weight-loaded forced swimming test, compared with a control group, CME and and PME groups could prolong exhaustive swimming time, increase levels of liver glycogen (LG) and muscle glycogen (MG), accelerate fatty acid oxidation in serum to provide energy, eliminate the accumulation of blood lactic acid (BLA) and blood urea nitrogen (BUN), and decrease the serum biomarkers for muscle damage, such as lactate dehydrogenase (LDH) and creatine kinase (CK).
30120787	4	37	theme	lactic	899:904	arg1	BLA					912:914	BLA	912:914	BLA	912:914	Based on the results of a weight-loaded forced swimming test, compared with a control group, CME and and PME groups could prolong exhaustive swimming time, increase levels of liver glycogen (LG) and muscle glycogen (MG), accelerate fatty acid oxidation in serum to provide energy, eliminate the accumulation of blood lactic acid (BLA) and blood urea nitrogen (BUN), and decrease the serum biomarkers for muscle damage, such as lactate dehydrogenase (LDH) and creatine kinase (CK).
30120787	4	38	theme	muscle	986:991	arg1	damage					993:998	muscle damage	986:998	muscle damage	986:998	Based on the results of a weight-loaded forced swimming test, compared with a control group, CME and and PME groups could prolong exhaustive swimming time, increase levels of liver glycogen (LG) and muscle glycogen (MG), accelerate fatty acid oxidation in serum to provide energy, eliminate the accumulation of blood lactic acid (BLA) and blood urea nitrogen (BUN), and decrease the serum biomarkers for muscle damage, such as lactate dehydrogenase (LDH) and creatine kinase (CK).
30120787	0	39	theme	physical	30:37	arg1	fatigue					39:45	physical fatigue	30:45	physical fatigue	30:45	Two macamide extracts relieve physical fatigue by attenuating muscle damage in mice.
30120787	4	40	theme	blood	893:897	arg1	acid					906:909	blood lactic acid	893:909	blood lactic acid (BLA)	893:915	Based on the results of a weight-loaded forced swimming test, compared with a control group, CME and and PME groups could prolong exhaustive swimming time, increase levels of liver glycogen (LG) and muscle glycogen (MG), accelerate fatty acid oxidation in serum to provide energy, eliminate the accumulation of blood lactic acid (BLA) and blood urea nitrogen (BUN), and decrease the serum biomarkers for muscle damage, such as lactate dehydrogenase (LDH) and creatine kinase (CK).
30120787	4	40	theme	blood	893:897	arg1	BLA					912:914	BLA	912:914	BLA	912:914	Based on the results of a weight-loaded forced swimming test, compared with a control group, CME and and PME groups could prolong exhaustive swimming time, increase levels of liver glycogen (LG) and muscle glycogen (MG), accelerate fatty acid oxidation in serum to provide energy, eliminate the accumulation of blood lactic acid (BLA) and blood urea nitrogen (BUN), and decrease the serum biomarkers for muscle damage, such as lactate dehydrogenase (LDH) and creatine kinase (CK).
30120787	0	41	from	damage	69:74	arg1	mice					79:82	mice	79:82	mice	79:82	Two macamide extracts relieve physical fatigue by attenuating muscle damage in mice.
30120787	1	42	theme	various	202:208	arg1	bioactivities					210:222	their various bioactivities	196:222	their various bioactivities	196:222	BACKGROUND Macamides, the main active components contained in maca, have attracted increasing attention due to their various bioactivities.
30120787	4	43	theme	swimming	629:636	arg1	test					638:641	a weight-loaded forced swimming test	606:641	a weight-loaded forced swimming test	606:641	Based on the results of a weight-loaded forced swimming test, compared with a control group, CME and and PME groups could prolong exhaustive swimming time, increase levels of liver glycogen (LG) and muscle glycogen (MG), accelerate fatty acid oxidation in serum to provide energy, eliminate the accumulation of blood lactic acid (BLA) and blood urea nitrogen (BUN), and decrease the serum biomarkers for muscle damage, such as lactate dehydrogenase (LDH) and creatine kinase (CK).
30120787	0	44	theme	muscle	62:67	arg1	damage					69:74	muscle damage	62:74	muscle damage in mice	62:82	Two macamide extracts relieve physical fatigue by attenuating muscle damage in mice.
30120787	4	45	theme	glycogen	763:770	arg1	levels					747:752	increase levels	738:752	increase levels of liver glycogen (LG) and muscle glycogen (MG)	738:800	Based on the results of a weight-loaded forced swimming test, compared with a control group, CME and and PME groups could prolong exhaustive swimming time, increase levels of liver glycogen (LG) and muscle glycogen (MG), accelerate fatty acid oxidation in serum to provide energy, eliminate the accumulation of blood lactic acid (BLA) and blood urea nitrogen (BUN), and decrease the serum biomarkers for muscle damage, such as lactate dehydrogenase (LDH) and creatine kinase (CK).
30120787	3	46	dep	analysis	494:501	arg1	results					503:509	results	503:509	results	503:509	RESULTS The composition analysis results revealed that both CME and PME mainly contain eight kinds of macamide.
30120787	2	47	theme	PME	423:425	arg1	effects					404:410	the anti-fatigue effects	387:410	the anti-fatigue effects of CME and PME	387:425	In this study, crude macamide extract (CME) and purified macamide extract (PME) were prepared by enzyme-assisted extraction and macroporous resin separation, and the anti-fatigue effects of CME and PME were evaluated in a forced swimming model.
30120787	4	48	dep	Based	582:586	arg1	compared					644:651	compared	644:651	compared with a control group	644:672	Based on the results of a weight-loaded forced swimming test, compared with a control group, CME and and PME groups could prolong exhaustive swimming time, increase levels of liver glycogen (LG) and muscle glycogen (MG), accelerate fatty acid oxidation in serum to provide energy, eliminate the accumulation of blood lactic acid (BLA) and blood urea nitrogen (BUN), and decrease the serum biomarkers for muscle damage, such as lactate dehydrogenase (LDH) and creatine kinase (CK).
30120787	3	49	dep	RESULTS	470:476	arg1	analysis					494:501	The composition analysis	478:501	RESULTS The composition analysis results	470:509	RESULTS The composition analysis results revealed that both CME and PME mainly contain eight kinds of macamide.
30120787	4	50	theme	creatine	1041:1048	arg1	CK					1058:1059	CK	1058:1059	CK	1058:1059	Based on the results of a weight-loaded forced swimming test, compared with a control group, CME and and PME groups could prolong exhaustive swimming time, increase levels of liver glycogen (LG) and muscle glycogen (MG), accelerate fatty acid oxidation in serum to provide energy, eliminate the accumulation of blood lactic acid (BLA) and blood urea nitrogen (BUN), and decrease the serum biomarkers for muscle damage, such as lactate dehydrogenase (LDH) and creatine kinase (CK).
30120787	4	50	theme	creatine	1041:1048	arg1	kinase					1050:1055	creatine kinase	1041:1055	creatine kinase (CK)	1041:1060	Based on the results of a weight-loaded forced swimming test, compared with a control group, CME and and PME groups could prolong exhaustive swimming time, increase levels of liver glycogen (LG) and muscle glycogen (MG), accelerate fatty acid oxidation in serum to provide energy, eliminate the accumulation of blood lactic acid (BLA) and blood urea nitrogen (BUN), and decrease the serum biomarkers for muscle damage, such as lactate dehydrogenase (LDH) and creatine kinase (CK).
30120787	1	51	theme	main	111:114	arg1	components					123:132	the main active components	107:132	the main active components contained in maca	107:150	BACKGROUND Macamides, the main active components contained in maca, have attracted increasing attention due to their various bioactivities.
30120787	1	51	theme	main	111:114	arg1	Macamides					96:104	BACKGROUND Macamides	85:104	BACKGROUND Macamides	85:104	BACKGROUND Macamides, the main active components contained in maca, have attracted increasing attention due to their various bioactivities.
30120787	7	52	theme	Chemical	1432:1439	arg1	Industry					1441:1448	Chemical Industry	1432:1448	Chemical Industry	1432:1448	© 2018 Society of Chemical Industry.
30120787	4	53	theme	fatty	814:818	arg1	oxidation					825:833	fatty acid oxidation	814:833	fatty acid oxidation in serum to provide energy, eliminate the accumulation of blood lactic acid (BLA) and blood urea nitrogen (BUN), and decrease the serum biomarkers for muscle damage, such as lactate dehydrogenase (LDH) and creatine kinase (CK)	814:1060	Based on the results of a weight-loaded forced swimming test, compared with a control group, CME and and PME groups could prolong exhaustive swimming time, increase levels of liver glycogen (LG) and muscle glycogen (MG), accelerate fatty acid oxidation in serum to provide energy, eliminate the accumulation of blood lactic acid (BLA) and blood urea nitrogen (BUN), and decrease the serum biomarkers for muscle damage, such as lactate dehydrogenase (LDH) and creatine kinase (CK).
30120787	2	54	theme	macamide	246:253	arg1	CME					264:266	CME	264:266	CME	264:266	In this study, crude macamide extract (CME) and purified macamide extract (PME) were prepared by enzyme-assisted extraction and macroporous resin separation, and the anti-fatigue effects of CME and PME were evaluated in a forced swimming model.
30120787	2	54	theme	macamide	246:253	arg1	extract					255:261	crude macamide extract	240:261	crude macamide extract (CME)	240:267	In this study, crude macamide extract (CME) and purified macamide extract (PME) were prepared by enzyme-assisted extraction and macroporous resin separation, and the anti-fatigue effects of CME and PME were evaluated in a forced swimming model.
30120787	4	55	theme	test	638:641	arg1	results					595:601	the results	591:601	the results of a weight-loaded forced swimming test	591:641	Based on the results of a weight-loaded forced swimming test, compared with a control group, CME and and PME groups could prolong exhaustive swimming time, increase levels of liver glycogen (LG) and muscle glycogen (MG), accelerate fatty acid oxidation in serum to provide energy, eliminate the accumulation of blood lactic acid (BLA) and blood urea nitrogen (BUN), and decrease the serum biomarkers for muscle damage, such as lactate dehydrogenase (LDH) and creatine kinase (CK).
30120787	1	56	theme	active	116:121	arg1	components					123:132	the main active components	107:132	the main active components contained in maca	107:150	BACKGROUND Macamides, the main active components contained in maca, have attracted increasing attention due to their various bioactivities.
30120787	1	56	theme	active	116:121	arg1	Macamides					96:104	BACKGROUND Macamides	85:104	BACKGROUND Macamides	85:104	BACKGROUND Macamides, the main active components contained in maca, have attracted increasing attention due to their various bioactivities.
30120787	4	57	theme	PME	687:689	arg1	groups					691:696	and PME groups	683:696	groups	691:696	Based on the results of a weight-loaded forced swimming test, compared with a control group, CME and and PME groups could prolong exhaustive swimming time, increase levels of liver glycogen (LG) and muscle glycogen (MG), accelerate fatty acid oxidation in serum to provide energy, eliminate the accumulation of blood lactic acid (BLA) and blood urea nitrogen (BUN), and decrease the serum biomarkers for muscle damage, such as lactate dehydrogenase (LDH) and creatine kinase (CK).
30120787	2	58	theme	crude	240:244	arg1	CME					264:266	CME	264:266	CME	264:266	In this study, crude macamide extract (CME) and purified macamide extract (PME) were prepared by enzyme-assisted extraction and macroporous resin separation, and the anti-fatigue effects of CME and PME were evaluated in a forced swimming model.
30120787	2	58	theme	crude	240:244	arg1	extract					255:261	crude macamide extract	240:261	crude macamide extract (CME)	240:267	In this study, crude macamide extract (CME) and purified macamide extract (PME) were prepared by enzyme-assisted extraction and macroporous resin separation, and the anti-fatigue effects of CME and PME were evaluated in a forced swimming model.
30120787	4	59	theme	lactate	1009:1015	arg1	dehydrogenase					1017:1029	lactate dehydrogenase	1009:1029	lactate dehydrogenase (LDH)	1009:1035	Based on the results of a weight-loaded forced swimming test, compared with a control group, CME and and PME groups could prolong exhaustive swimming time, increase levels of liver glycogen (LG) and muscle glycogen (MG), accelerate fatty acid oxidation in serum to provide energy, eliminate the accumulation of blood lactic acid (BLA) and blood urea nitrogen (BUN), and decrease the serum biomarkers for muscle damage, such as lactate dehydrogenase (LDH) and creatine kinase (CK).
30120787	4	59	theme	lactate	1009:1015	arg1	LDH					1032:1034	LDH	1032:1034	LDH	1032:1034	Based on the results of a weight-loaded forced swimming test, compared with a control group, CME and and PME groups could prolong exhaustive swimming time, increase levels of liver glycogen (LG) and muscle glycogen (MG), accelerate fatty acid oxidation in serum to provide energy, eliminate the accumulation of blood lactic acid (BLA) and blood urea nitrogen (BUN), and decrease the serum biomarkers for muscle damage, such as lactate dehydrogenase (LDH) and creatine kinase (CK).
30120787	3	60	theme	composition	482:492	arg1	analysis					494:501	The composition analysis	478:501	RESULTS The composition analysis results	470:509	RESULTS The composition analysis results revealed that both CME and PME mainly contain eight kinds of macamide.
30120787	5	61	theme	Histological	1063:1074	arg1	analysis					1076:1083	Histological analysis	1063:1083	Histological analysis	1063:1083	Histological analysis also indicated that CME and PME attenuated damage to skeletal muscle and the myocardium in mice during exercise.
30120787	4	62	theme	increase	738:745	arg1	levels					747:752	increase levels	738:752	increase levels of liver glycogen (LG) and muscle glycogen (MG)	738:800	Based on the results of a weight-loaded forced swimming test, compared with a control group, CME and and PME groups could prolong exhaustive swimming time, increase levels of liver glycogen (LG) and muscle glycogen (MG), accelerate fatty acid oxidation in serum to provide energy, eliminate the accumulation of blood lactic acid (BLA) and blood urea nitrogen (BUN), and decrease the serum biomarkers for muscle damage, such as lactate dehydrogenase (LDH) and creatine kinase (CK).
30120787	6	63	theme	PME	1403:1405	arg1	group					1407:1411	the PME group	1399:1411	the PME group	1399:1411	CONCLUSION Two macamide extracts have a beneficial effect on relieving physical fatigue by attenuating the damage of skeletal muscle and myocardium during exercise, and a better effect was observed in the PME group.
30120787	6	64	theme	beneficial	1238:1247	arg1	effect					1249:1254	a beneficial effect	1236:1254	a beneficial effect	1236:1254	CONCLUSION Two macamide extracts have a beneficial effect on relieving physical fatigue by attenuating the damage of skeletal muscle and myocardium during exercise, and a better effect was observed in the PME group.
30120787	6	65	theme	muscle	1324:1329	arg1	damage					1305:1310	the damage	1301:1310	the damage of skeletal muscle and myocardium	1301:1344	CONCLUSION Two macamide extracts have a beneficial effect on relieving physical fatigue by attenuating the damage of skeletal muscle and myocardium during exercise, and a better effect was observed in the PME group.
30120787	2	66	theme	swimming	454:461	arg1	model					463:467	a forced swimming model	445:467	a forced swimming model	445:467	In this study, crude macamide extract (CME) and purified macamide extract (PME) were prepared by enzyme-assisted extraction and macroporous resin separation, and the anti-fatigue effects of CME and PME were evaluated in a forced swimming model.
30120787	3	67	theme	macamide	572:579	arg1	kinds					563:567	eight kinds	557:567	eight kinds of macamide	557:579	RESULTS The composition analysis results revealed that both CME and PME mainly contain eight kinds of macamide.
30120787	2	68	theme	enzyme-assisted	322:336	arg1	extraction					338:347	enzyme-assisted extraction	322:347	enzyme-assisted extraction	322:347	In this study, crude macamide extract (CME) and purified macamide extract (PME) were prepared by enzyme-assisted extraction and macroporous resin separation, and the anti-fatigue effects of CME and PME were evaluated in a forced swimming model.
30120787	4	69	theme	nitrogen	932:939	arg1	accumulation					877:888	the accumulation	873:888	the accumulation of blood lactic acid (BLA) and blood urea nitrogen (BUN)	873:945	Based on the results of a weight-loaded forced swimming test, compared with a control group, CME and and PME groups could prolong exhaustive swimming time, increase levels of liver glycogen (LG) and muscle glycogen (MG), accelerate fatty acid oxidation in serum to provide energy, eliminate the accumulation of blood lactic acid (BLA) and blood urea nitrogen (BUN), and decrease the serum biomarkers for muscle damage, such as lactate dehydrogenase (LDH) and creatine kinase (CK).
29798909	7	0	theme	strong	1331:1336	arg1	correlations					1338:1349	strong correlations	1331:1349	strong correlations between blood vessel density and T-cell density in syngeneic tumors	1331:1417	Registration of ultrasound and confocal microscopy data showed strong correlations between blood vessel density and T-cell density in syngeneic tumors, while no correlations were found for genetic tumor models.
29798909	4	1	theme	ultrasound	582:591	arg1	system					593:598	A modified clinical ultrasound system	562:598	A modified clinical ultrasound system	562:598	We therefore set out to quantify ECM and stroma density and their relation to tumor stiffness.Experimental Design: A modified clinical ultrasound system was used to measure tumor stiffness and perfusion during tumor growth in preclinical tumor models.
29798909	5	2	with	tumors	1037:1042	arg1	collagen					1053:1060	low collagen and myofibroblast content	1049:1086	collagen	1053:1060	In vivo measurements were compared with collagen mass spectroscopy and automatic analysis of matrix and stromal markers derived from immunofluorescence images.Results: US elastography estimates of tumor stiffness were positively correlated with tumor volume in collagen and myofibroblast-rich tumors, while no correlations were found for tumors with low collagen and myofibroblast content.
29798909	5	2	with	tumors	1037:1042	arg1	content					1080:1086	low collagen and myofibroblast content	1049:1086	content	1080:1086	In vivo measurements were compared with collagen mass spectroscopy and automatic analysis of matrix and stromal markers derived from immunofluorescence images.Results: US elastography estimates of tumor stiffness were positively correlated with tumor volume in collagen and myofibroblast-rich tumors, while no correlations were found for tumors with low collagen and myofibroblast content.
29798909	1	3	theme	tissue	248:253	arg1	stiffness					255:263	tissue stiffness	248:263	tissue stiffness	248:263	Purpose: The tumor microenvironment presents with altered extracellular matrix (ECM) and stroma composition, which may affect treatment efficacy and contribute to tissue stiffness.
29798909	6	4	theme	collagen	1247:1254	arg1	crosslinks					1256:1265	immature collagen crosslinks	1238:1265	immature collagen crosslinks	1238:1265	US elastography measurements were strongly correlated with ex vivo mechanical testing and mass spectroscopy-based measurements of total collagen and immature collagen crosslinks.
29798909	2	5	theme	Ultrasound	266:275	arg1	elastography					282:293	Ultrasound (US) elastography	266:293	Ultrasound (US) elastography	266:293	Ultrasound (US) elastography can visualize and quantify tissue stiffness noninvasively.
29798909	4	6	from	growth	663:668	arg1	models					691:696	preclinical tumor models	673:696	preclinical tumor models	673:696	We therefore set out to quantify ECM and stroma density and their relation to tumor stiffness.Experimental Design: A modified clinical ultrasound system was used to measure tumor stiffness and perfusion during tumor growth in preclinical tumor models.
29798909	5	7	theme	tumor	896:900	arg1	stiffness					902:910	tumor stiffness	896:910	tumor stiffness	896:910	In vivo measurements were compared with collagen mass spectroscopy and automatic analysis of matrix and stromal markers derived from immunofluorescence images.Results: US elastography estimates of tumor stiffness were positively correlated with tumor volume in collagen and myofibroblast-rich tumors, while no correlations were found for tumors with low collagen and myofibroblast content.
29798909	7	8	from	density	1372:1378	arg1	tumors					1412:1417	syngeneic tumors	1402:1417	syngeneic tumors	1402:1417	Registration of ultrasound and confocal microscopy data showed strong correlations between blood vessel density and T-cell density in syngeneic tumors, while no correlations were found for genetic tumor models.
29798909	4	9	theme	preclinical	673:683	arg1	models					691:696	preclinical tumor models	673:696	preclinical tumor models	673:696	We therefore set out to quantify ECM and stroma density and their relation to tumor stiffness.Experimental Design: A modified clinical ultrasound system was used to measure tumor stiffness and perfusion during tumor growth in preclinical tumor models.
29798909	6	10	theme	total	1219:1223	arg1	collagen					1225:1232	total collagen	1219:1232	total collagen	1219:1232	US elastography measurements were strongly correlated with ex vivo mechanical testing and mass spectroscopy-based measurements of total collagen and immature collagen crosslinks.
29798909	5	11	theme	US	867:868	arg1	estimates					883:891	US elastography estimates	867:891	US elastography estimates of tumor stiffness	867:910	In vivo measurements were compared with collagen mass spectroscopy and automatic analysis of matrix and stromal markers derived from immunofluorescence images.Results: US elastography estimates of tumor stiffness were positively correlated with tumor volume in collagen and myofibroblast-rich tumors, while no correlations were found for tumors with low collagen and myofibroblast content.
29798909	9	12	theme	potential	1824:1832	arg1	biomarker					1834:1842	a potential biomarker	1822:1842	a potential biomarker to assess changes in the tumor microenvironment, particularly changes affecting the ECM	1822:1930	Finally, localized delivery of collagenase led to a significant reduction in tumor stiffness without changes in perfusion 24 hours after treatment.Conclusions: US elastography can be used as a potential biomarker to assess changes in the tumor microenvironment, particularly changes affecting the ECM.
29798909	9	12	theme	potential	1824:1832	arg1	elastography					1794:1805	US elastography	1791:1805	US elastography	1791:1805	Finally, localized delivery of collagenase led to a significant reduction in tumor stiffness without changes in perfusion 24 hours after treatment.Conclusions: US elastography can be used as a potential biomarker to assess changes in the tumor microenvironment, particularly changes affecting the ECM.
29798909	7	13	theme	tumor	1465:1469	arg1	models					1471:1476	genetic tumor models	1457:1476	genetic tumor models	1457:1476	Registration of ultrasound and confocal microscopy data showed strong correlations between blood vessel density and T-cell density in syngeneic tumors, while no correlations were found for genetic tumor models.
29798909	5	14	attach	derived	819:825	arg2	markers					811:817	matrix and stromal markers	792:817	matrix and stromal markers derived from immunofluorescence images.Results	792:864	In vivo measurements were compared with collagen mass spectroscopy and automatic analysis of matrix and stromal markers derived from immunofluorescence images.Results: US elastography estimates of tumor stiffness were positively correlated with tumor volume in collagen and myofibroblast-rich tumors, while no correlations were found for tumors with low collagen and myofibroblast content.
29798909	5	14	attach	derived	819:825	arg1	images.Results					851:864	immunofluorescence images.Results	832:864	immunofluorescence images.Results	832:864	In vivo measurements were compared with collagen mass spectroscopy and automatic analysis of matrix and stromal markers derived from immunofluorescence images.Results: US elastography estimates of tumor stiffness were positively correlated with tumor volume in collagen and myofibroblast-rich tumors, while no correlations were found for tumors with low collagen and myofibroblast content.
29798909	5	15	theme	In	699:700	arg1	measurements					707:718	In vivo measurements	699:718	In vivo measurements	699:718	In vivo measurements were compared with collagen mass spectroscopy and automatic analysis of matrix and stromal markers derived from immunofluorescence images.Results: US elastography estimates of tumor stiffness were positively correlated with tumor volume in collagen and myofibroblast-rich tumors, while no correlations were found for tumors with low collagen and myofibroblast content.
29798909	1	16	theme	altered	135:141	arg1	matrix					157:162	altered extracellular matrix	135:162	altered extracellular matrix (ECM)	135:168	Purpose: The tumor microenvironment presents with altered extracellular matrix (ECM) and stroma composition, which may affect treatment efficacy and contribute to tissue stiffness.
29798909	1	16	theme	altered	135:141	arg1	ECM					165:167	ECM	165:167	ECM	165:167	Purpose: The tumor microenvironment presents with altered extracellular matrix (ECM) and stroma composition, which may affect treatment efficacy and contribute to tissue stiffness.
29798909	5	17	theme	automatic	770:778	arg1	analysis					780:787	automatic analysis	770:787	automatic analysis	770:787	In vivo measurements were compared with collagen mass spectroscopy and automatic analysis of matrix and stromal markers derived from immunofluorescence images.Results: US elastography estimates of tumor stiffness were positively correlated with tumor volume in collagen and myofibroblast-rich tumors, while no correlations were found for tumors with low collagen and myofibroblast content.
29798909	7	18	theme	microscopy	1308:1317	arg1	Registration					1268:1279	Registration	1268:1279	Registration of ultrasound and confocal microscopy data	1268:1322	Registration of ultrasound and confocal microscopy data showed strong correlations between blood vessel density and T-cell density in syngeneic tumors, while no correlations were found for genetic tumor models.
29798909	5	19	theme	tumor	944:948	arg1	volume					950:955	tumor volume	944:955	tumor volume in collagen and myofibroblast-rich tumors	944:997	In vivo measurements were compared with collagen mass spectroscopy and automatic analysis of matrix and stromal markers derived from immunofluorescence images.Results: US elastography estimates of tumor stiffness were positively correlated with tumor volume in collagen and myofibroblast-rich tumors, while no correlations were found for tumors with low collagen and myofibroblast content.
29798909	8	20	theme	hyaluronic	1606:1615	arg1	density					1622:1628	hyaluronic acid density	1606:1628	hyaluronic acid density	1606:1628	In contrast to collagen density, which was positively correlated with stiffness, no significant correlations were observed for hyaluronic acid density.
29798909	9	21	theme	significant	1683:1693	arg1	reduction					1695:1703	a significant reduction	1681:1703	a significant reduction in tumor stiffness	1681:1722	Finally, localized delivery of collagenase led to a significant reduction in tumor stiffness without changes in perfusion 24 hours after treatment.Conclusions: US elastography can be used as a potential biomarker to assess changes in the tumor microenvironment, particularly changes affecting the ECM.
29798909	5	22	theme	collagen	739:746	arg1	spectroscopy					753:764	collagen mass spectroscopy	739:764	collagen mass spectroscopy	739:764	In vivo measurements were compared with collagen mass spectroscopy and automatic analysis of matrix and stromal markers derived from immunofluorescence images.Results: US elastography estimates of tumor stiffness were positively correlated with tumor volume in collagen and myofibroblast-rich tumors, while no correlations were found for tumors with low collagen and myofibroblast content.
29798909	8	23	located	observed	1593:1600	arg1	contrast					1482:1489	contrast	1482:1489	contrast	1482:1489	In contrast to collagen density, which was positively correlated with stiffness, no significant correlations were observed for hyaluronic acid density.
29798909	8	23	located	observed	1593:1600	arg2	correlations					1575:1586	no significant correlations	1560:1586	no significant correlations	1560:1586	In contrast to collagen density, which was positively correlated with stiffness, no significant correlations were observed for hyaluronic acid density.
29798909	9	24	theme	collagenase	1662:1672	arg1	delivery					1650:1657	localized delivery	1640:1657	localized delivery of collagenase	1640:1672	Finally, localized delivery of collagenase led to a significant reduction in tumor stiffness without changes in perfusion 24 hours after treatment.Conclusions: US elastography can be used as a potential biomarker to assess changes in the tumor microenvironment, particularly changes affecting the ECM.
29798909	3	25	theme	ECM	384:386	arg1	components					400:409	ECM and stromal components	384:409	ECM and stromal components	384:409	However, the contributions of ECM and stromal components to stiffness are poorly understood.
29798909	0	26	theme	Tumor	0:4	arg1	Elastography					6:17	Tumor Elastography	0:17	Tumor Elastography	0:17	Tumor Elastography and Its Association with Collagen and the Tumor Microenvironment.
29798909	5	27	from	volume	950:955	arg1	collagen					960:967	collagen	960:967	collagen	960:967	In vivo measurements were compared with collagen mass spectroscopy and automatic analysis of matrix and stromal markers derived from immunofluorescence images.Results: US elastography estimates of tumor stiffness were positively correlated with tumor volume in collagen and myofibroblast-rich tumors, while no correlations were found for tumors with low collagen and myofibroblast content.
29798909	5	27	from	volume	950:955	arg1	tumors					992:997	myofibroblast-rich tumors	973:997	myofibroblast-rich tumors	973:997	In vivo measurements were compared with collagen mass spectroscopy and automatic analysis of matrix and stromal markers derived from immunofluorescence images.Results: US elastography estimates of tumor stiffness were positively correlated with tumor volume in collagen and myofibroblast-rich tumors, while no correlations were found for tumors with low collagen and myofibroblast content.
29798909	1	28	theme	tumor	98:102	arg1	microenvironment					104:119	The tumor microenvironment	94:119	The tumor microenvironment	94:119	Purpose: The tumor microenvironment presents with altered extracellular matrix (ECM) and stroma composition, which may affect treatment efficacy and contribute to tissue stiffness.
29798909	9	29	from	reduction	1695:1703	arg1	stiffness					1714:1722	tumor stiffness	1708:1722	tumor stiffness	1708:1722	Finally, localized delivery of collagenase led to a significant reduction in tumor stiffness without changes in perfusion 24 hours after treatment.Conclusions: US elastography can be used as a potential biomarker to assess changes in the tumor microenvironment, particularly changes affecting the ECM.
29798909	6	30	theme	elastography	1092:1103	arg1	measurements					1105:1116	US elastography measurements	1089:1116	US elastography measurements	1089:1116	US elastography measurements were strongly correlated with ex vivo mechanical testing and mass spectroscopy-based measurements of total collagen and immature collagen crosslinks.
29798909	7	31	from	density	1391:1397	arg1	tumors					1412:1417	syngeneic tumors	1402:1417	syngeneic tumors	1402:1417	Registration of ultrasound and confocal microscopy data showed strong correlations between blood vessel density and T-cell density in syngeneic tumors, while no correlations were found for genetic tumor models.
29798909	3	32	theme	stromal	392:398	arg1	components					400:409	ECM and stromal components	384:409	ECM and stromal components	384:409	However, the contributions of ECM and stromal components to stiffness are poorly understood.
29798909	5	33	theme	markers	811:817	arg1	spectroscopy					753:764	collagen mass spectroscopy	739:764	collagen mass spectroscopy	739:764	In vivo measurements were compared with collagen mass spectroscopy and automatic analysis of matrix and stromal markers derived from immunofluorescence images.Results: US elastography estimates of tumor stiffness were positively correlated with tumor volume in collagen and myofibroblast-rich tumors, while no correlations were found for tumors with low collagen and myofibroblast content.
29798909	5	33	theme	markers	811:817	arg1	analysis					780:787	automatic analysis	770:787	automatic analysis	770:787	In vivo measurements were compared with collagen mass spectroscopy and automatic analysis of matrix and stromal markers derived from immunofluorescence images.Results: US elastography estimates of tumor stiffness were positively correlated with tumor volume in collagen and myofibroblast-rich tumors, while no correlations were found for tumors with low collagen and myofibroblast content.
29798909	7	34	theme	T-cell	1384:1389	arg1	density					1391:1397	T-cell density	1384:1397	T-cell density	1384:1397	Registration of ultrasound and confocal microscopy data showed strong correlations between blood vessel density and T-cell density in syngeneic tumors, while no correlations were found for genetic tumor models.
29798909	4	35	theme	tumor	525:529	arg1	Design					554:559	tumor stiffness.Experimental Design	525:559	tumor stiffness.Experimental Design	525:559	We therefore set out to quantify ECM and stroma density and their relation to tumor stiffness.Experimental Design: A modified clinical ultrasound system was used to measure tumor stiffness and perfusion during tumor growth in preclinical tumor models.
29798909	7	36	dep	ultrasound	1284:1293	arg1	data					1319:1322	data	1319:1322	data	1319:1322	Registration of ultrasound and confocal microscopy data showed strong correlations between blood vessel density and T-cell density in syngeneic tumors, while no correlations were found for genetic tumor models.
29798909	2	37	theme	tissue	322:327	arg1	stiffness					329:337	tissue stiffness	322:337	tissue stiffness	322:337	Ultrasound (US) elastography can visualize and quantify tissue stiffness noninvasively.
29798909	1	38	theme	treatment	211:219	arg1	efficacy					221:228	treatment efficacy	211:228	treatment efficacy	211:228	Purpose: The tumor microenvironment presents with altered extracellular matrix (ECM) and stroma composition, which may affect treatment efficacy and contribute to tissue stiffness.
29798909	9	39	used	used	1814:1817	arg2	biomarker					1834:1842	a potential biomarker	1822:1842	a potential biomarker to assess changes in the tumor microenvironment, particularly changes affecting the ECM	1822:1930	Finally, localized delivery of collagenase led to a significant reduction in tumor stiffness without changes in perfusion 24 hours after treatment.Conclusions: US elastography can be used as a potential biomarker to assess changes in the tumor microenvironment, particularly changes affecting the ECM.
29798909	9	39	used	used	1814:1817	arg2	elastography					1794:1805	US elastography	1791:1805	US elastography	1791:1805	Finally, localized delivery of collagenase led to a significant reduction in tumor stiffness without changes in perfusion 24 hours after treatment.Conclusions: US elastography can be used as a potential biomarker to assess changes in the tumor microenvironment, particularly changes affecting the ECM.
29798909	2	40	dep	Ultrasound	266:275	arg1	US					278:279	US	278:279	US	278:279	Ultrasound (US) elastography can visualize and quantify tissue stiffness noninvasively.
29798909	9	41	theme	perfusion	1743:1751	arg1	hours					1756:1760	perfusion 24 hours	1743:1760	perfusion 24 hours after treatment.Conclusions	1743:1788	Finally, localized delivery of collagenase led to a significant reduction in tumor stiffness without changes in perfusion 24 hours after treatment.Conclusions: US elastography can be used as a potential biomarker to assess changes in the tumor microenvironment, particularly changes affecting the ECM.
29798909	7	42	theme	blood	1359:1363	arg1	density					1372:1378	blood vessel density	1359:1378	blood vessel density	1359:1378	Registration of ultrasound and confocal microscopy data showed strong correlations between blood vessel density and T-cell density in syngeneic tumors, while no correlations were found for genetic tumor models.
29798909	0	43	with	Association	27:37	arg1	Collagen					44:51	Collagen	44:51	Collagen	44:51	Tumor Elastography and Its Association with Collagen and the Tumor Microenvironment.
29798909	6	44	theme	ex	1148:1149	arg1	testing					1167:1173	ex vivo mechanical testing	1148:1173	ex vivo mechanical testing	1148:1173	US elastography measurements were strongly correlated with ex vivo mechanical testing and mass spectroscopy-based measurements of total collagen and immature collagen crosslinks.
29798909	4	45	theme	clinical	573:580	arg1	system					593:598	A modified clinical ultrasound system	562:598	A modified clinical ultrasound system	562:598	We therefore set out to quantify ECM and stroma density and their relation to tumor stiffness.Experimental Design: A modified clinical ultrasound system was used to measure tumor stiffness and perfusion during tumor growth in preclinical tumor models.
29798909	0	46	with	Microenvironment	67:82	arg1	Collagen					44:51	Collagen	44:51	Collagen	44:51	Tumor Elastography and Its Association with Collagen and the Tumor Microenvironment.
29798909	6	47	theme	crosslinks	1256:1265	arg1	testing					1167:1173	ex vivo mechanical testing	1148:1173	ex vivo mechanical testing	1148:1173	US elastography measurements were strongly correlated with ex vivo mechanical testing and mass spectroscopy-based measurements of total collagen and immature collagen crosslinks.
29798909	6	47	theme	crosslinks	1256:1265	arg1	measurements					1203:1214	mass spectroscopy-based measurements	1179:1214	mass spectroscopy-based measurements	1179:1214	US elastography measurements were strongly correlated with ex vivo mechanical testing and mass spectroscopy-based measurements of total collagen and immature collagen crosslinks.
29798909	5	48	theme	stiffness	902:910	arg1	estimates					883:891	US elastography estimates	867:891	US elastography estimates of tumor stiffness	867:910	In vivo measurements were compared with collagen mass spectroscopy and automatic analysis of matrix and stromal markers derived from immunofluorescence images.Results: US elastography estimates of tumor stiffness were positively correlated with tumor volume in collagen and myofibroblast-rich tumors, while no correlations were found for tumors with low collagen and myofibroblast content.
29798909	9	49	theme	US	1791:1792	arg1	biomarker					1834:1842	a potential biomarker	1822:1842	a potential biomarker to assess changes in the tumor microenvironment, particularly changes affecting the ECM	1822:1930	Finally, localized delivery of collagenase led to a significant reduction in tumor stiffness without changes in perfusion 24 hours after treatment.Conclusions: US elastography can be used as a potential biomarker to assess changes in the tumor microenvironment, particularly changes affecting the ECM.
29798909	9	49	theme	US	1791:1792	arg1	elastography					1794:1805	US elastography	1791:1805	US elastography	1791:1805	Finally, localized delivery of collagenase led to a significant reduction in tumor stiffness without changes in perfusion 24 hours after treatment.Conclusions: US elastography can be used as a potential biomarker to assess changes in the tumor microenvironment, particularly changes affecting the ECM.
29798909	6	50	theme	immature	1238:1245	arg1	crosslinks					1256:1265	immature collagen crosslinks	1238:1265	immature collagen crosslinks	1238:1265	US elastography measurements were strongly correlated with ex vivo mechanical testing and mass spectroscopy-based measurements of total collagen and immature collagen crosslinks.
29798909	4	51	theme	tumor	657:661	arg1	growth					663:668	tumor growth	657:668	tumor growth in preclinical tumor models	657:696	We therefore set out to quantify ECM and stroma density and their relation to tumor stiffness.Experimental Design: A modified clinical ultrasound system was used to measure tumor stiffness and perfusion during tumor growth in preclinical tumor models.
29798909	1	52	theme	stroma	174:179	arg1	composition					181:191	stroma composition	174:191	stroma composition	174:191	Purpose: The tumor microenvironment presents with altered extracellular matrix (ECM) and stroma composition, which may affect treatment efficacy and contribute to tissue stiffness.
29798909	6	53	theme	collagen	1225:1232	arg1	testing					1167:1173	ex vivo mechanical testing	1148:1173	ex vivo mechanical testing	1148:1173	US elastography measurements were strongly correlated with ex vivo mechanical testing and mass spectroscopy-based measurements of total collagen and immature collagen crosslinks.
29798909	6	53	theme	collagen	1225:1232	arg1	measurements					1203:1214	mass spectroscopy-based measurements	1179:1214	mass spectroscopy-based measurements	1179:1214	US elastography measurements were strongly correlated with ex vivo mechanical testing and mass spectroscopy-based measurements of total collagen and immature collagen crosslinks.
29798909	10	54	dep	Res	1945:1947	arg1	4455-67					1958:1964	4455-67	1958:1964	4455-67	1958:1964	Clin Cancer Res; 24(18); 4455-67.
29798909	5	55	theme	myofibroblast	1066:1078	arg1	content					1080:1086	low collagen and myofibroblast content	1049:1086	content	1080:1086	In vivo measurements were compared with collagen mass spectroscopy and automatic analysis of matrix and stromal markers derived from immunofluorescence images.Results: US elastography estimates of tumor stiffness were positively correlated with tumor volume in collagen and myofibroblast-rich tumors, while no correlations were found for tumors with low collagen and myofibroblast content.
29798909	6	56	theme	spectroscopy-based	1184:1201	arg1	measurements					1203:1214	mass spectroscopy-based measurements	1179:1214	mass spectroscopy-based measurements	1179:1214	US elastography measurements were strongly correlated with ex vivo mechanical testing and mass spectroscopy-based measurements of total collagen and immature collagen crosslinks.
29798909	4	57	used	used	604:607	arg2	system					593:598	A modified clinical ultrasound system	562:598	A modified clinical ultrasound system	562:598	We therefore set out to quantify ECM and stroma density and their relation to tumor stiffness.Experimental Design: A modified clinical ultrasound system was used to measure tumor stiffness and perfusion during tumor growth in preclinical tumor models.
29798909	5	58	theme	elastography	870:881	arg1	estimates					883:891	US elastography estimates	867:891	US elastography estimates of tumor stiffness	867:910	In vivo measurements were compared with collagen mass spectroscopy and automatic analysis of matrix and stromal markers derived from immunofluorescence images.Results: US elastography estimates of tumor stiffness were positively correlated with tumor volume in collagen and myofibroblast-rich tumors, while no correlations were found for tumors with low collagen and myofibroblast content.
29798909	8	59	theme	significant	1563:1573	arg1	correlations					1575:1586	no significant correlations	1560:1586	no significant correlations	1560:1586	In contrast to collagen density, which was positively correlated with stiffness, no significant correlations were observed for hyaluronic acid density.
29798909	7	60	theme	genetic	1457:1463	arg1	models					1471:1476	genetic tumor models	1457:1476	genetic tumor models	1457:1476	Registration of ultrasound and confocal microscopy data showed strong correlations between blood vessel density and T-cell density in syngeneic tumors, while no correlations were found for genetic tumor models.
29798909	7	61	theme	vessel	1365:1370	arg1	density					1372:1378	blood vessel density	1359:1378	blood vessel density	1359:1378	Registration of ultrasound and confocal microscopy data showed strong correlations between blood vessel density and T-cell density in syngeneic tumors, while no correlations were found for genetic tumor models.
29798909	0	62	theme	Tumor	61:65	arg1	Microenvironment					67:82	the Tumor Microenvironment	57:82	the Tumor Microenvironment	57:82	Tumor Elastography and Its Association with Collagen and the Tumor Microenvironment.
29798909	1	63	theme	extracellular	143:155	arg1	matrix					157:162	altered extracellular matrix	135:162	altered extracellular matrix (ECM)	135:168	Purpose: The tumor microenvironment presents with altered extracellular matrix (ECM) and stroma composition, which may affect treatment efficacy and contribute to tissue stiffness.
29798909	1	63	theme	extracellular	143:155	arg1	ECM					165:167	ECM	165:167	ECM	165:167	Purpose: The tumor microenvironment presents with altered extracellular matrix (ECM) and stroma composition, which may affect treatment efficacy and contribute to tissue stiffness.
29798909	4	64	theme	tumor	685:689	arg1	models					691:696	preclinical tumor models	673:696	preclinical tumor models	673:696	We therefore set out to quantify ECM and stroma density and their relation to tumor stiffness.Experimental Design: A modified clinical ultrasound system was used to measure tumor stiffness and perfusion during tumor growth in preclinical tumor models.
29798909	7	65	theme	confocal	1299:1306	arg1	microscopy					1308:1317	confocal microscopy	1299:1317	confocal microscopy	1299:1317	Registration of ultrasound and confocal microscopy data showed strong correlations between blood vessel density and T-cell density in syngeneic tumors, while no correlations were found for genetic tumor models.
29798909	9	66	theme	tumor	1869:1873	arg1	microenvironment					1875:1890	the tumor microenvironment	1865:1890	the tumor microenvironment	1865:1890	Finally, localized delivery of collagenase led to a significant reduction in tumor stiffness without changes in perfusion 24 hours after treatment.Conclusions: US elastography can be used as a potential biomarker to assess changes in the tumor microenvironment, particularly changes affecting the ECM.
29798909	9	66	theme	tumor	1869:1873	arg1	changes					1906:1912	changes	1906:1912	particularly changes	1893:1912	Finally, localized delivery of collagenase led to a significant reduction in tumor stiffness without changes in perfusion 24 hours after treatment.Conclusions: US elastography can be used as a potential biomarker to assess changes in the tumor microenvironment, particularly changes affecting the ECM.
29798909	8	67	theme	acid	1617:1620	arg1	density					1622:1628	hyaluronic acid density	1606:1628	hyaluronic acid density	1606:1628	In contrast to collagen density, which was positively correlated with stiffness, no significant correlations were observed for hyaluronic acid density.
29798909	9	68	from	changes	1854:1860	arg1	microenvironment					1875:1890	the tumor microenvironment	1865:1890	the tumor microenvironment	1865:1890	Finally, localized delivery of collagenase led to a significant reduction in tumor stiffness without changes in perfusion 24 hours after treatment.Conclusions: US elastography can be used as a potential biomarker to assess changes in the tumor microenvironment, particularly changes affecting the ECM.
29798909	9	68	from	changes	1854:1860	arg1	changes					1906:1912	changes	1906:1912	particularly changes	1893:1912	Finally, localized delivery of collagenase led to a significant reduction in tumor stiffness without changes in perfusion 24 hours after treatment.Conclusions: US elastography can be used as a potential biomarker to assess changes in the tumor microenvironment, particularly changes affecting the ECM.
29798909	7	69	theme	ultrasound	1284:1293	arg1	Registration					1268:1279	Registration	1268:1279	Registration of ultrasound and confocal microscopy data	1268:1322	Registration of ultrasound and confocal microscopy data showed strong correlations between blood vessel density and T-cell density in syngeneic tumors, while no correlations were found for genetic tumor models.
29798909	9	70	theme	localized	1640:1648	arg1	delivery					1650:1657	localized delivery	1640:1657	localized delivery of collagenase	1640:1672	Finally, localized delivery of collagenase led to a significant reduction in tumor stiffness without changes in perfusion 24 hours after treatment.Conclusions: US elastography can be used as a potential biomarker to assess changes in the tumor microenvironment, particularly changes affecting the ECM.
29798909	5	71	dep	In	699:700	arg1	vivo					702:705	vivo	702:705	vivo	702:705	In vivo measurements were compared with collagen mass spectroscopy and automatic analysis of matrix and stromal markers derived from immunofluorescence images.Results: US elastography estimates of tumor stiffness were positively correlated with tumor volume in collagen and myofibroblast-rich tumors, while no correlations were found for tumors with low collagen and myofibroblast content.
29798909	5	72	theme	mass	748:751	arg1	spectroscopy					753:764	collagen mass spectroscopy	739:764	collagen mass spectroscopy	739:764	In vivo measurements were compared with collagen mass spectroscopy and automatic analysis of matrix and stromal markers derived from immunofluorescence images.Results: US elastography estimates of tumor stiffness were positively correlated with tumor volume in collagen and myofibroblast-rich tumors, while no correlations were found for tumors with low collagen and myofibroblast content.
29798909	5	73	theme	myofibroblast-rich	973:990	arg1	tumors					992:997	myofibroblast-rich tumors	973:997	myofibroblast-rich tumors	973:997	In vivo measurements were compared with collagen mass spectroscopy and automatic analysis of matrix and stromal markers derived from immunofluorescence images.Results: US elastography estimates of tumor stiffness were positively correlated with tumor volume in collagen and myofibroblast-rich tumors, while no correlations were found for tumors with low collagen and myofibroblast content.
29798909	9	74	from	changes	1732:1738	arg1	hours					1756:1760	perfusion 24 hours	1743:1760	perfusion 24 hours after treatment.Conclusions	1743:1788	Finally, localized delivery of collagenase led to a significant reduction in tumor stiffness without changes in perfusion 24 hours after treatment.Conclusions: US elastography can be used as a potential biomarker to assess changes in the tumor microenvironment, particularly changes affecting the ECM.
29798909	5	75	theme	immunofluorescence	832:849	arg1	images.Results					851:864	immunofluorescence images.Results	832:864	immunofluorescence images.Results	832:864	In vivo measurements were compared with collagen mass spectroscopy and automatic analysis of matrix and stromal markers derived from immunofluorescence images.Results: US elastography estimates of tumor stiffness were positively correlated with tumor volume in collagen and myofibroblast-rich tumors, while no correlations were found for tumors with low collagen and myofibroblast content.
29798909	4	76	dep	ECM	480:482	arg1	density					495:501	density	495:501	density	495:501	We therefore set out to quantify ECM and stroma density and their relation to tumor stiffness.Experimental Design: A modified clinical ultrasound system was used to measure tumor stiffness and perfusion during tumor growth in preclinical tumor models.
29798909	7	77	theme	syngeneic	1402:1410	arg1	tumors					1412:1417	syngeneic tumors	1402:1417	syngeneic tumors	1402:1417	Registration of ultrasound and confocal microscopy data showed strong correlations between blood vessel density and T-cell density in syngeneic tumors, while no correlations were found for genetic tumor models.
29798909	4	78	theme	modified	564:571	arg1	system					593:598	A modified clinical ultrasound system	562:598	A modified clinical ultrasound system	562:598	We therefore set out to quantify ECM and stroma density and their relation to tumor stiffness.Experimental Design: A modified clinical ultrasound system was used to measure tumor stiffness and perfusion during tumor growth in preclinical tumor models.
29798909	5	79	theme	matrix	792:797	arg1	markers					811:817	matrix and stromal markers	792:817	matrix and stromal markers derived from immunofluorescence images.Results	792:864	In vivo measurements were compared with collagen mass spectroscopy and automatic analysis of matrix and stromal markers derived from immunofluorescence images.Results: US elastography estimates of tumor stiffness were positively correlated with tumor volume in collagen and myofibroblast-rich tumors, while no correlations were found for tumors with low collagen and myofibroblast content.
29798909	6	80	theme	US	1089:1090	arg1	measurements					1105:1116	US elastography measurements	1089:1116	US elastography measurements	1089:1116	US elastography measurements were strongly correlated with ex vivo mechanical testing and mass spectroscopy-based measurements of total collagen and immature collagen crosslinks.
29798909	6	81	dep	ex	1148:1149	arg1	vivo					1151:1154	vivo	1151:1154	vivo	1151:1154	US elastography measurements were strongly correlated with ex vivo mechanical testing and mass spectroscopy-based measurements of total collagen and immature collagen crosslinks.
29798909	5	82	theme	stromal	803:809	arg1	markers					811:817	matrix and stromal markers	792:817	matrix and stromal markers derived from immunofluorescence images.Results	792:864	In vivo measurements were compared with collagen mass spectroscopy and automatic analysis of matrix and stromal markers derived from immunofluorescence images.Results: US elastography estimates of tumor stiffness were positively correlated with tumor volume in collagen and myofibroblast-rich tumors, while no correlations were found for tumors with low collagen and myofibroblast content.
29798909	9	83	theme	tumor	1708:1712	arg1	stiffness					1714:1722	tumor stiffness	1708:1722	tumor stiffness	1708:1722	Finally, localized delivery of collagenase led to a significant reduction in tumor stiffness without changes in perfusion 24 hours after treatment.Conclusions: US elastography can be used as a potential biomarker to assess changes in the tumor microenvironment, particularly changes affecting the ECM.
29798909	3	84	theme	components	400:409	arg1	contributions					367:379	the contributions	363:379	the contributions of ECM and stromal components to stiffness	363:422	However, the contributions of ECM and stromal components to stiffness are poorly understood.
29798909	4	85	theme	stiffness.Experimental	531:552	arg1	Design					554:559	tumor stiffness.Experimental Design	525:559	tumor stiffness.Experimental Design	525:559	We therefore set out to quantify ECM and stroma density and their relation to tumor stiffness.Experimental Design: A modified clinical ultrasound system was used to measure tumor stiffness and perfusion during tumor growth in preclinical tumor models.
29798909	8	86	theme	collagen	1494:1501	arg1	density					1503:1509	collagen density	1494:1509	collagen density	1494:1509	In contrast to collagen density, which was positively correlated with stiffness, no significant correlations were observed for hyaluronic acid density.
29798909	0	87	with	Elastography	6:17	arg1	Collagen					44:51	Collagen	44:51	Collagen	44:51	Tumor Elastography and Its Association with Collagen and the Tumor Microenvironment.
29798909	5	88	theme	low	1049:1051	arg1	collagen					1053:1060	low collagen and myofibroblast content	1049:1086	collagen	1053:1060	In vivo measurements were compared with collagen mass spectroscopy and automatic analysis of matrix and stromal markers derived from immunofluorescence images.Results: US elastography estimates of tumor stiffness were positively correlated with tumor volume in collagen and myofibroblast-rich tumors, while no correlations were found for tumors with low collagen and myofibroblast content.
29798909	6	89	theme	mass	1179:1182	arg1	measurements					1203:1214	mass spectroscopy-based measurements	1179:1214	mass spectroscopy-based measurements	1179:1214	US elastography measurements were strongly correlated with ex vivo mechanical testing and mass spectroscopy-based measurements of total collagen and immature collagen crosslinks.
29798909	6	90	theme	mechanical	1156:1165	arg1	testing					1167:1173	ex vivo mechanical testing	1148:1173	ex vivo mechanical testing	1148:1173	US elastography measurements were strongly correlated with ex vivo mechanical testing and mass spectroscopy-based measurements of total collagen and immature collagen crosslinks.
29798909	4	91	theme	tumor	620:624	arg1	stiffness					626:634	tumor stiffness	620:634	tumor stiffness	620:634	We therefore set out to quantify ECM and stroma density and their relation to tumor stiffness.Experimental Design: A modified clinical ultrasound system was used to measure tumor stiffness and perfusion during tumor growth in preclinical tumor models.
29798909	11	92	theme	©2018	1967:1971	arg1	AACR					1973:1976	©2018 AACR	1967:1976	©2018 AACR.	1967:1977	©2018 AACR.
29535731	11	0	from	changes	1976:1982	arg1	interactions					2023:2034	microbiome interactions	2012:2034	microbiome interactions	2012:2034	The altered IEC function and metabolisms leads to changes in barrier permeability and microbiome interactions, in turn, promoting greater translocation and inflammation and resulting in an increased disease severity.
29535731	11	0	from	changes	1976:1982	arg1	permeability					1995:2006	barrier permeability	1987:2006	barrier permeability	1987:2006	The altered IEC function and metabolisms leads to changes in barrier permeability and microbiome interactions, in turn, promoting greater translocation and inflammation and resulting in an increased disease severity.
29535731	7	1	theme	Nlrx1-/-	1187:1194	arg1	mice					1196:1199	Nlrx1-/- mice	1187:1199	Nlrx1-/- mice	1187:1199	Microbiome analyses demonstrate that colitogenic bacterial taxa such as Veillonella and Clostridiales are increased in abundance in Nlrx1-/- mice and in WT mice co-housed with Nlrx1-/- mice.
29535731	2	2	theme	exacerbated	304:314	arg1	severity					324:331	exacerbated disease severity	304:331	exacerbated disease severity	304:331	Nlrx1-/- mice have exacerbated disease severity, colonic lesions, and increased inflammatory markers.
29535731	9	3	theme	glutamine	1519:1527	arg1	profiles					1539:1546	WT glutamine metabolic profiles	1516:1546	WT glutamine metabolic profiles	1516:1546	To validate NLRX1 effects on IEC metabolism mediate gut-microbiome interactions, restoration of WT glutamine metabolic profiles through either exogenous glutamine supplementation or administration of 6-diazo-5-oxo-l-norleucine abrogates differences in inflammation, microbiome, and overall disease severity in Nlrx1-/- mice.
29535731	3	4	from	metabolism	534:543	arg1	mice					565:568	colitic Nlrx1-/- mice	548:568	colitic Nlrx1-/- mice	548:568	Global transcriptomic analyses demonstrate enhanced mucosal antimicrobial defense response, chemokine and cytokine expression, and epithelial cell metabolism in colitic Nlrx1-/- mice compared to wild-type (WT) mice.
29535731	1	5	theme	genetic	201:207	arg1	factors					209:215	genetic factors	201:215	genetic factors	201:215	Interactions among the gut microbiome, dysregulated immune responses, and genetic factors contribute to the pathogenesis of inflammatory bowel disease (IBD).
29535731	2	6	theme	colonic	334:340	arg1	lesions					342:348	colonic lesions	334:348	colonic lesions	334:348	Nlrx1-/- mice have exacerbated disease severity, colonic lesions, and increased inflammatory markers.
29535731	4	7	from	cells	707:711	arg1	loss					668:671	the loss	664:671	the loss of NLRX1 in intestinal epithelial cells (IEC)	664:717	Cell-specificity studies using cre-lox mice demonstrate that the loss of NLRX1 in intestinal epithelial cells (IEC) recapitulate the increased sensitivity to DSS colitis observed in whole body Nlrx1-/- mice.
29535731	5	8	theme	acid	927:930	arg1	metabolism					932:941	amino acid metabolism	921:941	amino acid metabolism	921:941	Further, organoid cultures of Nlrx1-/- and WT epithelial cells confirm the altered patterns of proliferation, amino acid metabolism, and tight junction expression.
29535731	4	9	theme	intestinal	685:694	arg1	IEC					714:716	IEC	714:716	IEC	714:716	Cell-specificity studies using cre-lox mice demonstrate that the loss of NLRX1 in intestinal epithelial cells (IEC) recapitulate the increased sensitivity to DSS colitis observed in whole body Nlrx1-/- mice.
29535731	4	9	theme	intestinal	685:694	arg1	cells					707:711	intestinal epithelial cells	685:711	intestinal epithelial cells (IEC)	685:717	Cell-specificity studies using cre-lox mice demonstrate that the loss of NLRX1 in intestinal epithelial cells (IEC) recapitulate the increased sensitivity to DSS colitis observed in whole body Nlrx1-/- mice.
29535731	9	10	theme	glutamine	1573:1581	arg1	supplementation					1583:1597	exogenous glutamine supplementation	1563:1597	exogenous glutamine supplementation	1563:1597	To validate NLRX1 effects on IEC metabolism mediate gut-microbiome interactions, restoration of WT glutamine metabolic profiles through either exogenous glutamine supplementation or administration of 6-diazo-5-oxo-l-norleucine abrogates differences in inflammation, microbiome, and overall disease severity in Nlrx1-/- mice.
29535731	8	11	theme	gut	1285:1287	arg1	microbiome					1289:1298	an Nlrx1-/--associated gut microbiome	1262:1298	an Nlrx1-/--associated gut microbiome	1262:1298	The transfer of an Nlrx1-/--associated gut microbiome through co-housing worsens disease in WT mice confirming the contributions of the microbiome to the Nlrx1-/- phenotype.
29535731	5	12	theme	expression	963:972	arg1	patterns					894:901	the altered patterns	882:901	the altered patterns of proliferation, amino acid metabolism, and tight junction expression	882:972	Further, organoid cultures of Nlrx1-/- and WT epithelial cells confirm the altered patterns of proliferation, amino acid metabolism, and tight junction expression.
29535731	12	13	theme	gut	2289:2291	arg1	microbiome					2293:2302	the gut microbiome	2285:2302	the gut microbiome	2285:2302	In conclusion, NLRX1 is an immunoregulatory molecule and a candidate modulator of the interplay between mucosal inflammation, metabolism, and the gut microbiome during IBD.
29535731	11	14	theme	barrier	1987:1993	arg1	permeability					1995:2006	barrier permeability	1987:2006	barrier permeability	1987:2006	The altered IEC function and metabolisms leads to changes in barrier permeability and microbiome interactions, in turn, promoting greater translocation and inflammation and resulting in an increased disease severity.
29535731	6	15	theme	IEC	996:998	arg1	behavior					1000:1007	IEC behavior	996:1007	IEC behavior	996:1007	These differences in IEC behavior can impact the composition of the microbiome.
29535731	10	16	theme	phenotype	1861:1869	arg1	deficiency					1765:1774	The influence NLRX1 deficiency	1745:1774	The influence NLRX1 deficiency on SIRT1-mediated effects	1745:1800	The influence NLRX1 deficiency on SIRT1-mediated effects is identified to be an upstream controller of the Nlrx1-/- phenotype in intestinal epithelial cell function and metabolism.
29535731	10	16	theme	phenotype	1861:1869	arg1	controller					1834:1843	an upstream controller	1822:1843	an upstream controller of the Nlrx1-/- phenotype in intestinal epithelial cell function and metabolism	1822:1923	The influence NLRX1 deficiency on SIRT1-mediated effects is identified to be an upstream controller of the Nlrx1-/- phenotype in intestinal epithelial cell function and metabolism.
29535731	10	17	theme	NLRX1	1759:1763	arg1	deficiency					1765:1774	The influence NLRX1 deficiency	1745:1774	The influence NLRX1 deficiency on SIRT1-mediated effects	1745:1800	The influence NLRX1 deficiency on SIRT1-mediated effects is identified to be an upstream controller of the Nlrx1-/- phenotype in intestinal epithelial cell function and metabolism.
29535731	10	17	theme	NLRX1	1759:1763	arg1	controller					1834:1843	an upstream controller	1822:1843	an upstream controller of the Nlrx1-/- phenotype in intestinal epithelial cell function and metabolism	1822:1923	The influence NLRX1 deficiency on SIRT1-mediated effects is identified to be an upstream controller of the Nlrx1-/- phenotype in intestinal epithelial cell function and metabolism.
29535731	11	18	theme	disease	2125:2131	arg1	severity					2133:2140	an increased disease severity	2112:2140	an increased disease severity	2112:2140	The altered IEC function and metabolisms leads to changes in barrier permeability and microbiome interactions, in turn, promoting greater translocation and inflammation and resulting in an increased disease severity.
29535731	7	19	theme	Microbiome	1055:1064	arg1	analyses					1066:1073	Microbiome analyses	1055:1073	Microbiome analyses	1055:1073	Microbiome analyses demonstrate that colitogenic bacterial taxa such as Veillonella and Clostridiales are increased in abundance in Nlrx1-/- mice and in WT mice co-housed with Nlrx1-/- mice.
29535731	1	20	theme	gut	150:152	arg1	microbiome					154:163	the gut microbiome	146:163	the gut microbiome	146:163	Interactions among the gut microbiome, dysregulated immune responses, and genetic factors contribute to the pathogenesis of inflammatory bowel disease (IBD).
29535731	8	21	theme	microbiome	1382:1391	arg1	contributions					1361:1373	the contributions	1357:1373	the contributions of the microbiome to the Nlrx1-/- phenotype	1357:1417	The transfer of an Nlrx1-/--associated gut microbiome through co-housing worsens disease in WT mice confirming the contributions of the microbiome to the Nlrx1-/- phenotype.
29535731	7	22	theme	bacterial	1104:1112	arg1	taxa					1114:1117	colitogenic bacterial taxa	1092:1117	colitogenic bacterial taxa such as Veillonella and Clostridiales	1092:1155	Microbiome analyses demonstrate that colitogenic bacterial taxa such as Veillonella and Clostridiales are increased in abundance in Nlrx1-/- mice and in WT mice co-housed with Nlrx1-/- mice.
29535731	7	22	theme	bacterial	1104:1112	arg1	Veillonella					1127:1137	Veillonella	1127:1137	Veillonella	1127:1137	Microbiome analyses demonstrate that colitogenic bacterial taxa such as Veillonella and Clostridiales are increased in abundance in Nlrx1-/- mice and in WT mice co-housed with Nlrx1-/- mice.
29535731	7	22	theme	bacterial	1104:1112	arg1	Clostridiales					1143:1155	Clostridiales	1143:1155	Clostridiales	1143:1155	Microbiome analyses demonstrate that colitogenic bacterial taxa such as Veillonella and Clostridiales are increased in abundance in Nlrx1-/- mice and in WT mice co-housed with Nlrx1-/- mice.
29535731	4	23	theme	increased	736:744	arg1	sensitivity					746:756	the increased sensitivity	732:756	the increased sensitivity to DSS colitis observed in whole body Nlrx1-/- mice	732:808	Cell-specificity studies using cre-lox mice demonstrate that the loss of NLRX1 in intestinal epithelial cells (IEC) recapitulate the increased sensitivity to DSS colitis observed in whole body Nlrx1-/- mice.
29535731	7	24	theme	Nlrx1-/-	1231:1238	arg1	mice					1240:1243	Nlrx1-/- mice	1231:1243	Nlrx1-/- mice	1231:1243	Microbiome analyses demonstrate that colitogenic bacterial taxa such as Veillonella and Clostridiales are increased in abundance in Nlrx1-/- mice and in WT mice co-housed with Nlrx1-/- mice.
29535731	1	25	theme	immune	179:184	arg1	responses					186:194	dysregulated immune responses	166:194	dysregulated immune responses	166:194	Interactions among the gut microbiome, dysregulated immune responses, and genetic factors contribute to the pathogenesis of inflammatory bowel disease (IBD).
29535731	12	26	from	molecule	2187:2194	arg1	conclusion					2146:2155	conclusion	2146:2155	conclusion	2146:2155	In conclusion, NLRX1 is an immunoregulatory molecule and a candidate modulator of the interplay between mucosal inflammation, metabolism, and the gut microbiome during IBD.
29535731	11	27	theme	IEC	1938:1940	arg1	function					1942:1949	The altered IEC function	1926:1949	The altered IEC function	1926:1949	The altered IEC function and metabolisms leads to changes in barrier permeability and microbiome interactions, in turn, promoting greater translocation and inflammation and resulting in an increased disease severity.
29535731	0	28	theme	Inflammatory	99:110	arg1	Disease					118:124	Inflammatory Bowel Disease	99:124	Inflammatory Bowel Disease	99:124	NLRX1 Modulates Immunometabolic Mechanisms Controlling the Host-Gut Microbiota Interactions during Inflammatory Bowel Disease.
29535731	3	29	from	response	469:476	arg1	mice					565:568	colitic Nlrx1-/- mice	548:568	colitic Nlrx1-/- mice	548:568	Global transcriptomic analyses demonstrate enhanced mucosal antimicrobial defense response, chemokine and cytokine expression, and epithelial cell metabolism in colitic Nlrx1-/- mice compared to wild-type (WT) mice.
29535731	5	30	theme	tight	948:952	arg1	expression					963:972	tight junction expression	948:972	tight junction expression	948:972	Further, organoid cultures of Nlrx1-/- and WT epithelial cells confirm the altered patterns of proliferation, amino acid metabolism, and tight junction expression.
29535731	9	31	from	microbiome	1686:1695	arg1	mice					1739:1742	Nlrx1-/- mice	1730:1742	Nlrx1-/- mice	1730:1742	To validate NLRX1 effects on IEC metabolism mediate gut-microbiome interactions, restoration of WT glutamine metabolic profiles through either exogenous glutamine supplementation or administration of 6-diazo-5-oxo-l-norleucine abrogates differences in inflammation, microbiome, and overall disease severity in Nlrx1-/- mice.
29535731	6	32	theme	microbiome	1043:1052	arg1	composition					1024:1034	the composition	1020:1034	the composition of the microbiome	1020:1052	These differences in IEC behavior can impact the composition of the microbiome.
29535731	3	33	theme	Global	387:392	arg1	analyses					409:416	Global transcriptomic analyses	387:416	Global transcriptomic analyses	387:416	Global transcriptomic analyses demonstrate enhanced mucosal antimicrobial defense response, chemokine and cytokine expression, and epithelial cell metabolism in colitic Nlrx1-/- mice compared to wild-type (WT) mice.
29535731	10	34	from	phenotype	1861:1869	arg1	metabolism					1914:1923	metabolism	1914:1923	metabolism	1914:1923	The influence NLRX1 deficiency on SIRT1-mediated effects is identified to be an upstream controller of the Nlrx1-/- phenotype in intestinal epithelial cell function and metabolism.
29535731	10	34	from	phenotype	1861:1869	arg1	function					1901:1908	intestinal epithelial cell function	1874:1908	intestinal epithelial cell function	1874:1908	The influence NLRX1 deficiency on SIRT1-mediated effects is identified to be an upstream controller of the Nlrx1-/- phenotype in intestinal epithelial cell function and metabolism.
29535731	9	35	theme	Nlrx1-/-	1730:1737	arg1	mice					1739:1742	Nlrx1-/- mice	1730:1742	Nlrx1-/- mice	1730:1742	To validate NLRX1 effects on IEC metabolism mediate gut-microbiome interactions, restoration of WT glutamine metabolic profiles through either exogenous glutamine supplementation or administration of 6-diazo-5-oxo-l-norleucine abrogates differences in inflammation, microbiome, and overall disease severity in Nlrx1-/- mice.
29535731	10	36	theme	upstream	1825:1832	arg1	deficiency					1765:1774	The influence NLRX1 deficiency	1745:1774	The influence NLRX1 deficiency on SIRT1-mediated effects	1745:1800	The influence NLRX1 deficiency on SIRT1-mediated effects is identified to be an upstream controller of the Nlrx1-/- phenotype in intestinal epithelial cell function and metabolism.
29535731	10	36	theme	upstream	1825:1832	arg1	controller					1834:1843	an upstream controller	1822:1843	an upstream controller of the Nlrx1-/- phenotype in intestinal epithelial cell function and metabolism	1822:1923	The influence NLRX1 deficiency on SIRT1-mediated effects is identified to be an upstream controller of the Nlrx1-/- phenotype in intestinal epithelial cell function and metabolism.
29535731	9	37	theme	overall	1702:1708	arg1	severity					1718:1725	overall disease severity	1702:1725	overall disease severity	1702:1725	To validate NLRX1 effects on IEC metabolism mediate gut-microbiome interactions, restoration of WT glutamine metabolic profiles through either exogenous glutamine supplementation or administration of 6-diazo-5-oxo-l-norleucine abrogates differences in inflammation, microbiome, and overall disease severity in Nlrx1-/- mice.
29535731	3	38	theme	enhanced	430:437	arg1	response					469:476	enhanced mucosal antimicrobial defense response	430:476	enhanced mucosal antimicrobial defense response	430:476	Global transcriptomic analyses demonstrate enhanced mucosal antimicrobial defense response, chemokine and cytokine expression, and epithelial cell metabolism in colitic Nlrx1-/- mice compared to wild-type (WT) mice.
29535731	4	39	theme	body	791:794	arg1	mice					805:808	whole body Nlrx1-/- mice	785:808	whole body Nlrx1-/- mice	785:808	Cell-specificity studies using cre-lox mice demonstrate that the loss of NLRX1 in intestinal epithelial cells (IEC) recapitulate the increased sensitivity to DSS colitis observed in whole body Nlrx1-/- mice.
29535731	11	40	theme	greater	2056:2062	arg1	translocation					2064:2076	greater translocation	2056:2076	greater translocation	2056:2076	The altered IEC function and metabolisms leads to changes in barrier permeability and microbiome interactions, in turn, promoting greater translocation and inflammation and resulting in an increased disease severity.
29535731	5	41	theme	epithelial	857:866	arg1	cells					868:872	Nlrx1-/- and WT epithelial cells	841:872	Nlrx1-/- and WT epithelial cells	841:872	Further, organoid cultures of Nlrx1-/- and WT epithelial cells confirm the altered patterns of proliferation, amino acid metabolism, and tight junction expression.
29535731	3	42	theme	epithelial	518:527	arg1	metabolism					534:543	epithelial cell metabolism	518:543	epithelial cell metabolism	518:543	Global transcriptomic analyses demonstrate enhanced mucosal antimicrobial defense response, chemokine and cytokine expression, and epithelial cell metabolism in colitic Nlrx1-/- mice compared to wild-type (WT) mice.
29535731	0	43	theme	Immunometabolic	16:30	arg1	Mechanisms					32:41	Immunometabolic Mechanisms	16:41	Immunometabolic Mechanisms Controlling the Host-Gut Microbiota Interactions during Inflammatory Bowel Disease	16:124	NLRX1 Modulates Immunometabolic Mechanisms Controlling the Host-Gut Microbiota Interactions during Inflammatory Bowel Disease.
29535731	3	44	dep	wild-type	582:590	arg1	WT					593:594	WT	593:594	WT	593:594	Global transcriptomic analyses demonstrate enhanced mucosal antimicrobial defense response, chemokine and cytokine expression, and epithelial cell metabolism in colitic Nlrx1-/- mice compared to wild-type (WT) mice.
29535731	1	45	theme	dysregulated	166:177	arg1	responses					186:194	dysregulated immune responses	166:194	dysregulated immune responses	166:194	Interactions among the gut microbiome, dysregulated immune responses, and genetic factors contribute to the pathogenesis of inflammatory bowel disease (IBD).
29535731	10	46	from	metabolism	1914:1923	arg1	deficiency					1765:1774	The influence NLRX1 deficiency	1745:1774	The influence NLRX1 deficiency on SIRT1-mediated effects	1745:1800	The influence NLRX1 deficiency on SIRT1-mediated effects is identified to be an upstream controller of the Nlrx1-/- phenotype in intestinal epithelial cell function and metabolism.
29535731	10	46	from	metabolism	1914:1923	arg1	controller					1834:1843	an upstream controller	1822:1843	an upstream controller of the Nlrx1-/- phenotype in intestinal epithelial cell function and metabolism	1822:1923	The influence NLRX1 deficiency on SIRT1-mediated effects is identified to be an upstream controller of the Nlrx1-/- phenotype in intestinal epithelial cell function and metabolism.
29535731	9	47	theme	metabolic	1529:1537	arg1	profiles					1539:1546	WT glutamine metabolic profiles	1516:1546	WT glutamine metabolic profiles	1516:1546	To validate NLRX1 effects on IEC metabolism mediate gut-microbiome interactions, restoration of WT glutamine metabolic profiles through either exogenous glutamine supplementation or administration of 6-diazo-5-oxo-l-norleucine abrogates differences in inflammation, microbiome, and overall disease severity in Nlrx1-/- mice.
29535731	12	48	theme	immunoregulatory	2170:2185	arg1	molecule					2187:2194	an immunoregulatory molecule	2167:2194	an immunoregulatory molecule	2167:2194	In conclusion, NLRX1 is an immunoregulatory molecule and a candidate modulator of the interplay between mucosal inflammation, metabolism, and the gut microbiome during IBD.
29535731	12	48	theme	immunoregulatory	2170:2185	arg1	NLRX1					2158:2162	NLRX1	2158:2162	NLRX1	2158:2162	In conclusion, NLRX1 is an immunoregulatory molecule and a candidate modulator of the interplay between mucosal inflammation, metabolism, and the gut microbiome during IBD.
29535731	3	49	theme	antimicrobial	447:459	arg1	response					469:476	enhanced mucosal antimicrobial defense response	430:476	enhanced mucosal antimicrobial defense response	430:476	Global transcriptomic analyses demonstrate enhanced mucosal antimicrobial defense response, chemokine and cytokine expression, and epithelial cell metabolism in colitic Nlrx1-/- mice compared to wild-type (WT) mice.
29535731	3	50	theme	colitic	548:554	arg1	mice					565:568	colitic Nlrx1-/- mice	548:568	colitic Nlrx1-/- mice	548:568	Global transcriptomic analyses demonstrate enhanced mucosal antimicrobial defense response, chemokine and cytokine expression, and epithelial cell metabolism in colitic Nlrx1-/- mice compared to wild-type (WT) mice.
29535731	0	51	theme	Host-Gut	59:66	arg1	Interactions					79:90	the Host-Gut Microbiota Interactions	55:90	the Host-Gut Microbiota Interactions	55:90	NLRX1 Modulates Immunometabolic Mechanisms Controlling the Host-Gut Microbiota Interactions during Inflammatory Bowel Disease.
29535731	9	52	theme	IEC	1449:1451	arg1	metabolism					1453:1462	IEC metabolism	1449:1462	IEC metabolism	1449:1462	To validate NLRX1 effects on IEC metabolism mediate gut-microbiome interactions, restoration of WT glutamine metabolic profiles through either exogenous glutamine supplementation or administration of 6-diazo-5-oxo-l-norleucine abrogates differences in inflammation, microbiome, and overall disease severity in Nlrx1-/- mice.
29535731	1	53	theme	inflammatory	251:262	arg1	disease					270:276	inflammatory bowel disease	251:276	inflammatory bowel disease (IBD)	251:282	Interactions among the gut microbiome, dysregulated immune responses, and genetic factors contribute to the pathogenesis of inflammatory bowel disease (IBD).
29535731	1	53	theme	inflammatory	251:262	arg1	IBD					279:281	IBD	279:281	IBD	279:281	Interactions among the gut microbiome, dysregulated immune responses, and genetic factors contribute to the pathogenesis of inflammatory bowel disease (IBD).
29535731	10	54	theme	Nlrx1-/-	1852:1859	arg1	phenotype					1861:1869	the Nlrx1-/- phenotype	1848:1869	the Nlrx1-/- phenotype in intestinal epithelial cell function and metabolism	1848:1923	The influence NLRX1 deficiency on SIRT1-mediated effects is identified to be an upstream controller of the Nlrx1-/- phenotype in intestinal epithelial cell function and metabolism.
29535731	10	55	theme	cell	1896:1899	arg1	function					1901:1908	intestinal epithelial cell function	1874:1908	intestinal epithelial cell function	1874:1908	The influence NLRX1 deficiency on SIRT1-mediated effects is identified to be an upstream controller of the Nlrx1-/- phenotype in intestinal epithelial cell function and metabolism.
29535731	9	56	theme	profiles	1539:1546	arg1	restoration					1501:1511	restoration	1501:1511	restoration of WT glutamine metabolic profiles through either exogenous glutamine supplementation or administration of 6-diazo-5-oxo-l-norleucine	1501:1645	To validate NLRX1 effects on IEC metabolism mediate gut-microbiome interactions, restoration of WT glutamine metabolic profiles through either exogenous glutamine supplementation or administration of 6-diazo-5-oxo-l-norleucine abrogates differences in inflammation, microbiome, and overall disease severity in Nlrx1-/- mice.
29535731	1	57	theme	disease	270:276	arg1	pathogenesis					235:246	the pathogenesis	231:246	the pathogenesis of inflammatory bowel disease (IBD)	231:282	Interactions among the gut microbiome, dysregulated immune responses, and genetic factors contribute to the pathogenesis of inflammatory bowel disease (IBD).
29535731	0	58	theme	Microbiota	68:77	arg1	Interactions					79:90	the Host-Gut Microbiota Interactions	55:90	the Host-Gut Microbiota Interactions	55:90	NLRX1 Modulates Immunometabolic Mechanisms Controlling the Host-Gut Microbiota Interactions during Inflammatory Bowel Disease.
29535731	12	59	from	modulator	2212:2220	arg1	conclusion					2146:2155	conclusion	2146:2155	conclusion	2146:2155	In conclusion, NLRX1 is an immunoregulatory molecule and a candidate modulator of the interplay between mucosal inflammation, metabolism, and the gut microbiome during IBD.
29535731	10	60	from	controller	1834:1843	arg1	metabolism					1914:1923	metabolism	1914:1923	metabolism	1914:1923	The influence NLRX1 deficiency on SIRT1-mediated effects is identified to be an upstream controller of the Nlrx1-/- phenotype in intestinal epithelial cell function and metabolism.
29535731	10	60	from	controller	1834:1843	arg1	function					1901:1908	intestinal epithelial cell function	1874:1908	intestinal epithelial cell function	1874:1908	The influence NLRX1 deficiency on SIRT1-mediated effects is identified to be an upstream controller of the Nlrx1-/- phenotype in intestinal epithelial cell function and metabolism.
29535731	4	61	from	loss	668:671	arg1	IEC					714:716	IEC	714:716	IEC	714:716	Cell-specificity studies using cre-lox mice demonstrate that the loss of NLRX1 in intestinal epithelial cells (IEC) recapitulate the increased sensitivity to DSS colitis observed in whole body Nlrx1-/- mice.
29535731	4	61	from	loss	668:671	arg1	cells					707:711	intestinal epithelial cells	685:711	intestinal epithelial cells (IEC)	685:717	Cell-specificity studies using cre-lox mice demonstrate that the loss of NLRX1 in intestinal epithelial cells (IEC) recapitulate the increased sensitivity to DSS colitis observed in whole body Nlrx1-/- mice.
29535731	10	62	theme	intestinal	1874:1883	arg1	function					1901:1908	intestinal epithelial cell function	1874:1908	intestinal epithelial cell function	1874:1908	The influence NLRX1 deficiency on SIRT1-mediated effects is identified to be an upstream controller of the Nlrx1-/- phenotype in intestinal epithelial cell function and metabolism.
29535731	12	63	theme	candidate	2202:2210	arg1	modulator					2212:2220	a candidate modulator	2200:2220	a candidate modulator of the interplay between mucosal inflammation, metabolism, and the gut microbiome during IBD	2200:2313	In conclusion, NLRX1 is an immunoregulatory molecule and a candidate modulator of the interplay between mucosal inflammation, metabolism, and the gut microbiome during IBD.
29535731	9	64	from	inflammation	1672:1683	arg1	mice					1739:1742	Nlrx1-/- mice	1730:1742	Nlrx1-/- mice	1730:1742	To validate NLRX1 effects on IEC metabolism mediate gut-microbiome interactions, restoration of WT glutamine metabolic profiles through either exogenous glutamine supplementation or administration of 6-diazo-5-oxo-l-norleucine abrogates differences in inflammation, microbiome, and overall disease severity in Nlrx1-/- mice.
29535731	4	65	theme	cre-lox	634:640	arg1	mice					642:645	cre-lox mice	634:645	cre-lox mice	634:645	Cell-specificity studies using cre-lox mice demonstrate that the loss of NLRX1 in intestinal epithelial cells (IEC) recapitulate the increased sensitivity to DSS colitis observed in whole body Nlrx1-/- mice.
29535731	2	66	theme	disease	316:322	arg1	severity					324:331	exacerbated disease severity	304:331	exacerbated disease severity	304:331	Nlrx1-/- mice have exacerbated disease severity, colonic lesions, and increased inflammatory markers.
29535731	9	67	theme	WT	1516:1517	arg1	profiles					1539:1546	WT glutamine metabolic profiles	1516:1546	WT glutamine metabolic profiles	1516:1546	To validate NLRX1 effects on IEC metabolism mediate gut-microbiome interactions, restoration of WT glutamine metabolic profiles through either exogenous glutamine supplementation or administration of 6-diazo-5-oxo-l-norleucine abrogates differences in inflammation, microbiome, and overall disease severity in Nlrx1-/- mice.
29535731	10	68	from	deficiency	1765:1774	arg1	effects					1794:1800	SIRT1-mediated effects	1779:1800	SIRT1-mediated effects	1779:1800	The influence NLRX1 deficiency on SIRT1-mediated effects is identified to be an upstream controller of the Nlrx1-/- phenotype in intestinal epithelial cell function and metabolism.
29535731	9	69	from	differences	1657:1667	arg1	severity					1718:1725	overall disease severity	1702:1725	overall disease severity	1702:1725	To validate NLRX1 effects on IEC metabolism mediate gut-microbiome interactions, restoration of WT glutamine metabolic profiles through either exogenous glutamine supplementation or administration of 6-diazo-5-oxo-l-norleucine abrogates differences in inflammation, microbiome, and overall disease severity in Nlrx1-/- mice.
29535731	9	69	from	differences	1657:1667	arg1	microbiome					1686:1695	microbiome	1686:1695	microbiome	1686:1695	To validate NLRX1 effects on IEC metabolism mediate gut-microbiome interactions, restoration of WT glutamine metabolic profiles through either exogenous glutamine supplementation or administration of 6-diazo-5-oxo-l-norleucine abrogates differences in inflammation, microbiome, and overall disease severity in Nlrx1-/- mice.
29535731	9	69	from	differences	1657:1667	arg1	inflammation					1672:1683	inflammation	1672:1683	inflammation	1672:1683	To validate NLRX1 effects on IEC metabolism mediate gut-microbiome interactions, restoration of WT glutamine metabolic profiles through either exogenous glutamine supplementation or administration of 6-diazo-5-oxo-l-norleucine abrogates differences in inflammation, microbiome, and overall disease severity in Nlrx1-/- mice.
29535731	2	70	theme	inflammatory	365:376	arg1	markers					378:384	increased inflammatory markers	355:384	increased inflammatory markers	355:384	Nlrx1-/- mice have exacerbated disease severity, colonic lesions, and increased inflammatory markers.
29535731	5	71	theme	proliferation	906:918	arg1	patterns					894:901	the altered patterns	882:901	the altered patterns of proliferation, amino acid metabolism, and tight junction expression	882:972	Further, organoid cultures of Nlrx1-/- and WT epithelial cells confirm the altered patterns of proliferation, amino acid metabolism, and tight junction expression.
29535731	7	72	theme	WT	1208:1209	arg1	mice					1211:1214	WT mice	1208:1214	WT mice co-housed with Nlrx1-/- mice	1208:1243	Microbiome analyses demonstrate that colitogenic bacterial taxa such as Veillonella and Clostridiales are increased in abundance in Nlrx1-/- mice and in WT mice co-housed with Nlrx1-/- mice.
29535731	10	73	theme	influence	1749:1757	arg1	deficiency					1765:1774	The influence NLRX1 deficiency	1745:1774	The influence NLRX1 deficiency on SIRT1-mediated effects	1745:1800	The influence NLRX1 deficiency on SIRT1-mediated effects is identified to be an upstream controller of the Nlrx1-/- phenotype in intestinal epithelial cell function and metabolism.
29535731	10	73	theme	influence	1749:1757	arg1	controller					1834:1843	an upstream controller	1822:1843	an upstream controller of the Nlrx1-/- phenotype in intestinal epithelial cell function and metabolism	1822:1923	The influence NLRX1 deficiency on SIRT1-mediated effects is identified to be an upstream controller of the Nlrx1-/- phenotype in intestinal epithelial cell function and metabolism.
29535731	5	74	theme	organoid	820:827	arg1	cultures					829:836	organoid cultures	820:836	organoid cultures of Nlrx1-/- and WT epithelial cells	820:872	Further, organoid cultures of Nlrx1-/- and WT epithelial cells confirm the altered patterns of proliferation, amino acid metabolism, and tight junction expression.
29535731	5	75	theme	amino	921:925	arg1	metabolism					932:941	amino acid metabolism	921:941	amino acid metabolism	921:941	Further, organoid cultures of Nlrx1-/- and WT epithelial cells confirm the altered patterns of proliferation, amino acid metabolism, and tight junction expression.
29535731	9	76	theme	exogenous	1563:1571	arg1	supplementation					1583:1597	exogenous glutamine supplementation	1563:1597	exogenous glutamine supplementation	1563:1597	To validate NLRX1 effects on IEC metabolism mediate gut-microbiome interactions, restoration of WT glutamine metabolic profiles through either exogenous glutamine supplementation or administration of 6-diazo-5-oxo-l-norleucine abrogates differences in inflammation, microbiome, and overall disease severity in Nlrx1-/- mice.
29535731	12	77	theme	interplay	2229:2237	arg1	NLRX1					2158:2162	NLRX1	2158:2162	NLRX1	2158:2162	In conclusion, NLRX1 is an immunoregulatory molecule and a candidate modulator of the interplay between mucosal inflammation, metabolism, and the gut microbiome during IBD.
29535731	12	77	theme	interplay	2229:2237	arg1	molecule					2187:2194	an immunoregulatory molecule	2167:2194	an immunoregulatory molecule	2167:2194	In conclusion, NLRX1 is an immunoregulatory molecule and a candidate modulator of the interplay between mucosal inflammation, metabolism, and the gut microbiome during IBD.
29535731	12	77	theme	interplay	2229:2237	arg1	modulator					2212:2220	a candidate modulator	2200:2220	a candidate modulator of the interplay between mucosal inflammation, metabolism, and the gut microbiome during IBD	2200:2313	In conclusion, NLRX1 is an immunoregulatory molecule and a candidate modulator of the interplay between mucosal inflammation, metabolism, and the gut microbiome during IBD.
29535731	8	78	theme	WT	1338:1339	arg1	mice					1341:1344	WT mice	1338:1344	WT mice confirming the contributions of the microbiome to the Nlrx1-/- phenotype	1338:1417	The transfer of an Nlrx1-/--associated gut microbiome through co-housing worsens disease in WT mice confirming the contributions of the microbiome to the Nlrx1-/- phenotype.
29535731	10	79	theme	SIRT1-mediated	1779:1792	arg1	effects					1794:1800	SIRT1-mediated effects	1779:1800	SIRT1-mediated effects	1779:1800	The influence NLRX1 deficiency on SIRT1-mediated effects is identified to be an upstream controller of the Nlrx1-/- phenotype in intestinal epithelial cell function and metabolism.
29535731	2	80	theme	increased	355:363	arg1	markers					378:384	increased inflammatory markers	355:384	increased inflammatory markers	355:384	Nlrx1-/- mice have exacerbated disease severity, colonic lesions, and increased inflammatory markers.
29535731	8	81	theme	Nlrx1-/--associated	1265:1283	arg1	microbiome					1289:1298	an Nlrx1-/--associated gut microbiome	1262:1298	an Nlrx1-/--associated gut microbiome	1262:1298	The transfer of an Nlrx1-/--associated gut microbiome through co-housing worsens disease in WT mice confirming the contributions of the microbiome to the Nlrx1-/- phenotype.
29535731	4	82	theme	epithelial	696:705	arg1	IEC					714:716	IEC	714:716	IEC	714:716	Cell-specificity studies using cre-lox mice demonstrate that the loss of NLRX1 in intestinal epithelial cells (IEC) recapitulate the increased sensitivity to DSS colitis observed in whole body Nlrx1-/- mice.
29535731	4	82	theme	epithelial	696:705	arg1	cells					707:711	intestinal epithelial cells	685:711	intestinal epithelial cells (IEC)	685:717	Cell-specificity studies using cre-lox mice demonstrate that the loss of NLRX1 in intestinal epithelial cells (IEC) recapitulate the increased sensitivity to DSS colitis observed in whole body Nlrx1-/- mice.
29535731	11	83	theme	increased	2115:2123	arg1	severity					2133:2140	an increased disease severity	2112:2140	an increased disease severity	2112:2140	The altered IEC function and metabolisms leads to changes in barrier permeability and microbiome interactions, in turn, promoting greater translocation and inflammation and resulting in an increased disease severity.
29535731	5	84	theme	junction	954:961	arg1	expression					963:972	tight junction expression	948:972	tight junction expression	948:972	Further, organoid cultures of Nlrx1-/- and WT epithelial cells confirm the altered patterns of proliferation, amino acid metabolism, and tight junction expression.
29535731	8	85	theme	microbiome	1289:1298	arg1	transfer					1250:1257	The transfer	1246:1257	The transfer of an Nlrx1-/--associated gut microbiome through co-housing	1246:1317	The transfer of an Nlrx1-/--associated gut microbiome through co-housing worsens disease in WT mice confirming the contributions of the microbiome to the Nlrx1-/- phenotype.
29535731	12	86	theme	mucosal	2247:2253	arg1	inflammation					2255:2266	mucosal inflammation	2247:2266	mucosal inflammation	2247:2266	In conclusion, NLRX1 is an immunoregulatory molecule and a candidate modulator of the interplay between mucosal inflammation, metabolism, and the gut microbiome during IBD.
29535731	4	87	theme	DSS	761:763	arg1	colitis					765:771	DSS colitis	761:771	DSS colitis	761:771	Cell-specificity studies using cre-lox mice demonstrate that the loss of NLRX1 in intestinal epithelial cells (IEC) recapitulate the increased sensitivity to DSS colitis observed in whole body Nlrx1-/- mice.
29535731	7	88	theme	colitogenic	1092:1102	arg1	taxa					1114:1117	colitogenic bacterial taxa	1092:1117	colitogenic bacterial taxa such as Veillonella and Clostridiales	1092:1155	Microbiome analyses demonstrate that colitogenic bacterial taxa such as Veillonella and Clostridiales are increased in abundance in Nlrx1-/- mice and in WT mice co-housed with Nlrx1-/- mice.
29535731	7	88	theme	colitogenic	1092:1102	arg1	Veillonella					1127:1137	Veillonella	1127:1137	Veillonella	1127:1137	Microbiome analyses demonstrate that colitogenic bacterial taxa such as Veillonella and Clostridiales are increased in abundance in Nlrx1-/- mice and in WT mice co-housed with Nlrx1-/- mice.
29535731	7	88	theme	colitogenic	1092:1102	arg1	Clostridiales					1143:1155	Clostridiales	1143:1155	Clostridiales	1143:1155	Microbiome analyses demonstrate that colitogenic bacterial taxa such as Veillonella and Clostridiales are increased in abundance in Nlrx1-/- mice and in WT mice co-housed with Nlrx1-/- mice.
29535731	2	89	theme	Nlrx1-/-	285:292	arg1	mice					294:297	Nlrx1-/- mice	285:297	Nlrx1-/- mice	285:297	Nlrx1-/- mice have exacerbated disease severity, colonic lesions, and increased inflammatory markers.
29535731	9	90	theme	6-diazo-5-oxo-l-norleucine	1620:1645	arg1	administration					1602:1615	administration	1602:1615	administration of 6-diazo-5-oxo-l-norleucine	1602:1645	To validate NLRX1 effects on IEC metabolism mediate gut-microbiome interactions, restoration of WT glutamine metabolic profiles through either exogenous glutamine supplementation or administration of 6-diazo-5-oxo-l-norleucine abrogates differences in inflammation, microbiome, and overall disease severity in Nlrx1-/- mice.
29535731	9	90	theme	6-diazo-5-oxo-l-norleucine	1620:1645	arg1	supplementation					1583:1597	exogenous glutamine supplementation	1563:1597	exogenous glutamine supplementation	1563:1597	To validate NLRX1 effects on IEC metabolism mediate gut-microbiome interactions, restoration of WT glutamine metabolic profiles through either exogenous glutamine supplementation or administration of 6-diazo-5-oxo-l-norleucine abrogates differences in inflammation, microbiome, and overall disease severity in Nlrx1-/- mice.
29535731	10	91	from	function	1901:1908	arg1	deficiency					1765:1774	The influence NLRX1 deficiency	1745:1774	The influence NLRX1 deficiency on SIRT1-mediated effects	1745:1800	The influence NLRX1 deficiency on SIRT1-mediated effects is identified to be an upstream controller of the Nlrx1-/- phenotype in intestinal epithelial cell function and metabolism.
29535731	10	91	from	function	1901:1908	arg1	controller					1834:1843	an upstream controller	1822:1843	an upstream controller of the Nlrx1-/- phenotype in intestinal epithelial cell function and metabolism	1822:1923	The influence NLRX1 deficiency on SIRT1-mediated effects is identified to be an upstream controller of the Nlrx1-/- phenotype in intestinal epithelial cell function and metabolism.
29535731	4	92	from	NLRX1	676:680	arg1	IEC					714:716	IEC	714:716	IEC	714:716	Cell-specificity studies using cre-lox mice demonstrate that the loss of NLRX1 in intestinal epithelial cells (IEC) recapitulate the increased sensitivity to DSS colitis observed in whole body Nlrx1-/- mice.
29535731	4	92	from	NLRX1	676:680	arg1	cells					707:711	intestinal epithelial cells	685:711	intestinal epithelial cells (IEC)	685:717	Cell-specificity studies using cre-lox mice demonstrate that the loss of NLRX1 in intestinal epithelial cells (IEC) recapitulate the increased sensitivity to DSS colitis observed in whole body Nlrx1-/- mice.
29535731	5	93	theme	metabolism	932:941	arg1	patterns					894:901	the altered patterns	882:901	the altered patterns of proliferation, amino acid metabolism, and tight junction expression	882:972	Further, organoid cultures of Nlrx1-/- and WT epithelial cells confirm the altered patterns of proliferation, amino acid metabolism, and tight junction expression.
29535731	9	94	from	severity	1718:1725	arg1	mice					1739:1742	Nlrx1-/- mice	1730:1742	Nlrx1-/- mice	1730:1742	To validate NLRX1 effects on IEC metabolism mediate gut-microbiome interactions, restoration of WT glutamine metabolic profiles through either exogenous glutamine supplementation or administration of 6-diazo-5-oxo-l-norleucine abrogates differences in inflammation, microbiome, and overall disease severity in Nlrx1-/- mice.
29535731	0	95	theme	Bowel	112:116	arg1	Disease					118:124	Inflammatory Bowel Disease	99:124	Inflammatory Bowel Disease	99:124	NLRX1 Modulates Immunometabolic Mechanisms Controlling the Host-Gut Microbiota Interactions during Inflammatory Bowel Disease.
29535731	4	96	located	observed	773:780	arg1	mice					805:808	whole body Nlrx1-/- mice	785:808	whole body Nlrx1-/- mice	785:808	Cell-specificity studies using cre-lox mice demonstrate that the loss of NLRX1 in intestinal epithelial cells (IEC) recapitulate the increased sensitivity to DSS colitis observed in whole body Nlrx1-/- mice.
29535731	4	96	located	observed	773:780	arg2	sensitivity					746:756	the increased sensitivity	732:756	the increased sensitivity to DSS colitis observed in whole body Nlrx1-/- mice	732:808	Cell-specificity studies using cre-lox mice demonstrate that the loss of NLRX1 in intestinal epithelial cells (IEC) recapitulate the increased sensitivity to DSS colitis observed in whole body Nlrx1-/- mice.
29535731	8	97	theme	Nlrx1-/-	1400:1407	arg1	phenotype					1409:1417	the Nlrx1-/- phenotype	1396:1417	the Nlrx1-/- phenotype	1396:1417	The transfer of an Nlrx1-/--associated gut microbiome through co-housing worsens disease in WT mice confirming the contributions of the microbiome to the Nlrx1-/- phenotype.
29535731	9	98	from	effects	1438:1444	arg1	metabolism					1453:1462	IEC metabolism	1449:1462	IEC metabolism	1449:1462	To validate NLRX1 effects on IEC metabolism mediate gut-microbiome interactions, restoration of WT glutamine metabolic profiles through either exogenous glutamine supplementation or administration of 6-diazo-5-oxo-l-norleucine abrogates differences in inflammation, microbiome, and overall disease severity in Nlrx1-/- mice.
29535731	3	99	theme	transcriptomic	394:407	arg1	analyses					409:416	Global transcriptomic analyses	387:416	Global transcriptomic analyses	387:416	Global transcriptomic analyses demonstrate enhanced mucosal antimicrobial defense response, chemokine and cytokine expression, and epithelial cell metabolism in colitic Nlrx1-/- mice compared to wild-type (WT) mice.
29535731	11	100	theme	microbiome	2012:2021	arg1	interactions					2023:2034	microbiome interactions	2012:2034	microbiome interactions	2012:2034	The altered IEC function and metabolisms leads to changes in barrier permeability and microbiome interactions, in turn, promoting greater translocation and inflammation and resulting in an increased disease severity.
29535731	5	101	theme	altered	886:892	arg1	patterns					894:901	the altered patterns	882:901	the altered patterns of proliferation, amino acid metabolism, and tight junction expression	882:972	Further, organoid cultures of Nlrx1-/- and WT epithelial cells confirm the altered patterns of proliferation, amino acid metabolism, and tight junction expression.
29535731	11	102	theme	altered	1930:1936	arg1	function					1942:1949	The altered IEC function	1926:1949	The altered IEC function	1926:1949	The altered IEC function and metabolisms leads to changes in barrier permeability and microbiome interactions, in turn, promoting greater translocation and inflammation and resulting in an increased disease severity.
29535731	9	103	theme	gut-microbiome	1472:1485	arg1	interactions					1487:1498	gut-microbiome interactions	1472:1498	gut-microbiome interactions	1472:1498	To validate NLRX1 effects on IEC metabolism mediate gut-microbiome interactions, restoration of WT glutamine metabolic profiles through either exogenous glutamine supplementation or administration of 6-diazo-5-oxo-l-norleucine abrogates differences in inflammation, microbiome, and overall disease severity in Nlrx1-/- mice.
29535731	5	104	theme	WT	854:855	arg1	cells					868:872	Nlrx1-/- and WT epithelial cells	841:872	Nlrx1-/- and WT epithelial cells	841:872	Further, organoid cultures of Nlrx1-/- and WT epithelial cells confirm the altered patterns of proliferation, amino acid metabolism, and tight junction expression.
29535731	9	105	theme	disease	1710:1716	arg1	severity					1718:1725	overall disease severity	1702:1725	overall disease severity	1702:1725	To validate NLRX1 effects on IEC metabolism mediate gut-microbiome interactions, restoration of WT glutamine metabolic profiles through either exogenous glutamine supplementation or administration of 6-diazo-5-oxo-l-norleucine abrogates differences in inflammation, microbiome, and overall disease severity in Nlrx1-/- mice.
29535731	3	106	theme	mucosal	439:445	arg1	response					469:476	enhanced mucosal antimicrobial defense response	430:476	enhanced mucosal antimicrobial defense response	430:476	Global transcriptomic analyses demonstrate enhanced mucosal antimicrobial defense response, chemokine and cytokine expression, and epithelial cell metabolism in colitic Nlrx1-/- mice compared to wild-type (WT) mice.
29535731	4	107	theme	Cell-specificity	603:618	arg1	studies					620:626	Cell-specificity studies	603:626	Cell-specificity studies using cre-lox mice	603:645	Cell-specificity studies using cre-lox mice demonstrate that the loss of NLRX1 in intestinal epithelial cells (IEC) recapitulate the increased sensitivity to DSS colitis observed in whole body Nlrx1-/- mice.
29535731	4	108	theme	whole	785:789	arg1	mice					805:808	whole body Nlrx1-/- mice	785:808	whole body Nlrx1-/- mice	785:808	Cell-specificity studies using cre-lox mice demonstrate that the loss of NLRX1 in intestinal epithelial cells (IEC) recapitulate the increased sensitivity to DSS colitis observed in whole body Nlrx1-/- mice.
29535731	5	109	theme	cells	868:872	arg1	cultures					829:836	organoid cultures	820:836	organoid cultures of Nlrx1-/- and WT epithelial cells	820:872	Further, organoid cultures of Nlrx1-/- and WT epithelial cells confirm the altered patterns of proliferation, amino acid metabolism, and tight junction expression.
29535731	2	110	contain	have	299:302	arg2	lesions					342:348	colonic lesions	334:348	colonic lesions	334:348	Nlrx1-/- mice have exacerbated disease severity, colonic lesions, and increased inflammatory markers.
29535731	2	110	contain	have	299:302	arg1	mice					294:297	Nlrx1-/- mice	285:297	Nlrx1-/- mice	285:297	Nlrx1-/- mice have exacerbated disease severity, colonic lesions, and increased inflammatory markers.
29535731	2	110	contain	have	299:302	arg2	severity					324:331	exacerbated disease severity	304:331	exacerbated disease severity	304:331	Nlrx1-/- mice have exacerbated disease severity, colonic lesions, and increased inflammatory markers.
29535731	2	110	contain	have	299:302	arg2	markers					378:384	increased inflammatory markers	355:384	increased inflammatory markers	355:384	Nlrx1-/- mice have exacerbated disease severity, colonic lesions, and increased inflammatory markers.
29535731	4	111	theme	Nlrx1-/-	796:803	arg1	mice					805:808	whole body Nlrx1-/- mice	785:808	whole body Nlrx1-/- mice	785:808	Cell-specificity studies using cre-lox mice demonstrate that the loss of NLRX1 in intestinal epithelial cells (IEC) recapitulate the increased sensitivity to DSS colitis observed in whole body Nlrx1-/- mice.
29535731	3	112	theme	cell	529:532	arg1	metabolism					534:543	epithelial cell metabolism	518:543	epithelial cell metabolism	518:543	Global transcriptomic analyses demonstrate enhanced mucosal antimicrobial defense response, chemokine and cytokine expression, and epithelial cell metabolism in colitic Nlrx1-/- mice compared to wild-type (WT) mice.
29535731	9	113	theme	NLRX1	1432:1436	arg1	effects					1438:1444	NLRX1 effects	1432:1444	NLRX1 effects on IEC metabolism	1432:1462	To validate NLRX1 effects on IEC metabolism mediate gut-microbiome interactions, restoration of WT glutamine metabolic profiles through either exogenous glutamine supplementation or administration of 6-diazo-5-oxo-l-norleucine abrogates differences in inflammation, microbiome, and overall disease severity in Nlrx1-/- mice.
29535731	3	114	dep	chemokine	479:487	arg1	expression					502:511	expression	502:511	expression	502:511	Global transcriptomic analyses demonstrate enhanced mucosal antimicrobial defense response, chemokine and cytokine expression, and epithelial cell metabolism in colitic Nlrx1-/- mice compared to wild-type (WT) mice.
29535731	5	115	theme	Nlrx1-/-	841:848	arg1	cells					868:872	Nlrx1-/- and WT epithelial cells	841:872	Nlrx1-/- and WT epithelial cells	841:872	Further, organoid cultures of Nlrx1-/- and WT epithelial cells confirm the altered patterns of proliferation, amino acid metabolism, and tight junction expression.
29535731	3	116	theme	defense	461:467	arg1	response					469:476	enhanced mucosal antimicrobial defense response	430:476	enhanced mucosal antimicrobial defense response	430:476	Global transcriptomic analyses demonstrate enhanced mucosal antimicrobial defense response, chemokine and cytokine expression, and epithelial cell metabolism in colitic Nlrx1-/- mice compared to wild-type (WT) mice.
29535731	6	117	from	differences	981:991	arg1	behavior					1000:1007	IEC behavior	996:1007	IEC behavior	996:1007	These differences in IEC behavior can impact the composition of the microbiome.
29535731	3	118	theme	Nlrx1-/-	556:563	arg1	mice					565:568	colitic Nlrx1-/- mice	548:568	colitic Nlrx1-/- mice	548:568	Global transcriptomic analyses demonstrate enhanced mucosal antimicrobial defense response, chemokine and cytokine expression, and epithelial cell metabolism in colitic Nlrx1-/- mice compared to wild-type (WT) mice.
29535731	3	119	from	chemokine	479:487	arg1	mice					565:568	colitic Nlrx1-/- mice	548:568	colitic Nlrx1-/- mice	548:568	Global transcriptomic analyses demonstrate enhanced mucosal antimicrobial defense response, chemokine and cytokine expression, and epithelial cell metabolism in colitic Nlrx1-/- mice compared to wild-type (WT) mice.
29535731	10	120	theme	epithelial	1885:1894	arg1	function					1901:1908	intestinal epithelial cell function	1874:1908	intestinal epithelial cell function	1874:1908	The influence NLRX1 deficiency on SIRT1-mediated effects is identified to be an upstream controller of the Nlrx1-/- phenotype in intestinal epithelial cell function and metabolism.
29535731	1	121	theme	bowel	264:268	arg1	disease					270:276	inflammatory bowel disease	251:276	inflammatory bowel disease (IBD)	251:282	Interactions among the gut microbiome, dysregulated immune responses, and genetic factors contribute to the pathogenesis of inflammatory bowel disease (IBD).
29535731	1	121	theme	bowel	264:268	arg1	IBD					279:281	IBD	279:281	IBD	279:281	Interactions among the gut microbiome, dysregulated immune responses, and genetic factors contribute to the pathogenesis of inflammatory bowel disease (IBD).
29535731	4	122	theme	NLRX1	676:680	arg1	loss					668:671	the loss	664:671	the loss of NLRX1 in intestinal epithelial cells (IEC)	664:717	Cell-specificity studies using cre-lox mice demonstrate that the loss of NLRX1 in intestinal epithelial cells (IEC) recapitulate the increased sensitivity to DSS colitis observed in whole body Nlrx1-/- mice.
29535731	3	123	from	cytokine	493:500	arg1	mice					565:568	colitic Nlrx1-/- mice	548:568	colitic Nlrx1-/- mice	548:568	Global transcriptomic analyses demonstrate enhanced mucosal antimicrobial defense response, chemokine and cytokine expression, and epithelial cell metabolism in colitic Nlrx1-/- mice compared to wild-type (WT) mice.
29535731	3	124	theme	wild-type	582:590	arg1	mice					597:600	wild-type (WT) mice	582:600	wild-type (WT) mice	582:600	Global transcriptomic analyses demonstrate enhanced mucosal antimicrobial defense response, chemokine and cytokine expression, and epithelial cell metabolism in colitic Nlrx1-/- mice compared to wild-type (WT) mice.
29538391	0	0	theme	alloy	76:80	arg1	extract					82:88	biodegradable Mg-based alloy extract	53:88	biodegradable Mg-based alloy extract	53:88	In vitro study of the inflammatory cells response to biodegradable Mg-based alloy extract.
29538391	7	1	theme	middle	1071:1076	arg1	extracts					1101:1108	middle and high concentration extracts	1071:1108	middle and high concentration extracts independent of LPS	1071:1127	Interestingly, the production of TNF-α decreased when macrophages were cultured in middle and high concentration extracts independent of LPS.
29538391	1	2	theme	bone	151:154	arg1	devices					165:171	bone fixation devices	151:171	bone fixation devices	151:171	Biodegradable Mg-based alloys have shown great potential as bone fixation devices or vascular stents.
29538391	1	2	theme	bone	151:154	arg1	potential					138:146	great potential	132:146	great potential	132:146	Biodegradable Mg-based alloys have shown great potential as bone fixation devices or vascular stents.
29538391	9	3	theme	immune	1354:1359	arg1	cells					1361:1365	immune cells	1354:1365	immune cells	1354:1365	Our results provide new information about the impact of Mg alloy extracts on phenotype of immune cells and the potential mechanism, which should be taken into account prior to clinical applications.
29538391	0	4	theme	Mg-based	67:74	arg1	extract					82:88	biodegradable Mg-based alloy extract	53:88	biodegradable Mg-based alloy extract	53:88	In vitro study of the inflammatory cells response to biodegradable Mg-based alloy extract.
29538391	5	5	theme	middle-concentration	708:727	arg1	extract					729:735	middle-concentration extract	708:735	middle-concentration extract	708:735	For middle-concentration extract, cell apoptosis and partially impaired cell function were observed.
29538391	9	6	theme	clinical	1440:1447	arg1	applications					1449:1460	clinical applications	1440:1460	clinical applications	1440:1460	Our results provide new information about the impact of Mg alloy extracts on phenotype of immune cells and the potential mechanism, which should be taken into account prior to clinical applications.
29538391	8	7	theme	magnesium	1177:1185	arg1	ions					1187:1190	magnesium ions	1177:1190	magnesium ions in JDBM extracts, which was a potential factor affecting cell function	1177:1261	Cell viability was also negatively affected by magnesium ions in JDBM extracts, which was a potential factor affecting cell function.
29538391	1	8	theme	fixation	156:163	arg1	devices					165:171	bone fixation devices	151:171	bone fixation devices	151:171	Biodegradable Mg-based alloys have shown great potential as bone fixation devices or vascular stents.
29538391	1	8	theme	fixation	156:163	arg1	potential					138:146	great potential	132:146	great potential	132:146	Biodegradable Mg-based alloys have shown great potential as bone fixation devices or vascular stents.
29538391	9	9	theme	cells	1361:1365	arg1	phenotype					1341:1349	phenotype	1341:1349	phenotype of immune cells and the potential mechanism, which should be taken into account prior to clinical applications	1341:1460	Our results provide new information about the impact of Mg alloy extracts on phenotype of immune cells and the potential mechanism, which should be taken into account prior to clinical applications.
29538391	9	10	from	impact	1310:1315	arg1	phenotype					1341:1349	phenotype	1341:1349	phenotype of immune cells and the potential mechanism, which should be taken into account prior to clinical applications	1341:1460	Our results provide new information about the impact of Mg alloy extracts on phenotype of immune cells and the potential mechanism, which should be taken into account prior to clinical applications.
29538391	4	11	theme	function	694:701	arg1	damage					679:684	complete damage	670:684	complete damage	670:684	The results showed that high-concentration extract induced necrosis and complete damage of cell function.
29538391	4	11	theme	function	694:701	arg1	necrosis					657:664	necrosis	657:664	necrosis	657:664	The results showed that high-concentration extract induced necrosis and complete damage of cell function.
29538391	6	12	theme	same	933:936	arg1	concentration					938:950	the same concentration	929:950	the same concentration	929:950	TNF-α expression of macrophages was up-regulated by co-culture with extract in 20% concentration, but was down-regulated in the same concentration in the presence of LPS stimulation.
29538391	4	13	theme	complete	670:677	arg1	damage					679:684	complete damage	670:684	complete damage	670:684	The results showed that high-concentration extract induced necrosis and complete damage of cell function.
29538391	8	14	from	ions	1187:1190	arg1	extracts					1200:1207	JDBM extracts	1195:1207	JDBM extracts	1195:1207	Cell viability was also negatively affected by magnesium ions in JDBM extracts, which was a potential factor affecting cell function.
29538391	8	14	from	ions	1187:1190	arg1	factor					1232:1237	a potential factor	1220:1237	a potential factor affecting cell function	1220:1261	Cell viability was also negatively affected by magnesium ions in JDBM extracts, which was a potential factor affecting cell function.
29538391	4	15	theme	cell	689:692	arg1	function					694:701	cell function	689:701	cell function	689:701	The results showed that high-concentration extract induced necrosis and complete damage of cell function.
29538391	9	16	theme	extracts	1329:1336	arg1	impact					1310:1315	the impact	1306:1315	the impact of Mg alloy extracts on phenotype of immune cells and the potential mechanism, which should be taken into account prior to clinical applications	1306:1460	Our results provide new information about the impact of Mg alloy extracts on phenotype of immune cells and the potential mechanism, which should be taken into account prior to clinical applications.
29538391	2	17	theme	important	256:264	arg1	issue					266:270	an important issue to be studied, where the inflammatory cells play a key role	253:330	an important issue to be studied, where the inflammatory cells play a key role	253:330	As implant biomaterials, the foreign body reaction (FBR) is an important issue to be studied, where the inflammatory cells play a key role.
29538391	2	17	theme	important	256:264	arg1	reaction					235:242	the foreign body reaction	218:242	the foreign body reaction (FBR)	218:248	As implant biomaterials, the foreign body reaction (FBR) is an important issue to be studied, where the inflammatory cells play a key role.
29538391	8	18	theme	cell	1249:1252	arg1	function					1254:1261	cell function	1249:1261	cell function	1249:1261	Cell viability was also negatively affected by magnesium ions in JDBM extracts, which was a potential factor affecting cell function.
29538391	8	19	theme	Cell	1130:1133	arg1	viability					1135:1143	Cell viability	1130:1143	Cell viability	1130:1143	Cell viability was also negatively affected by magnesium ions in JDBM extracts, which was a potential factor affecting cell function.
29538391	7	20	theme	LPS	1125:1127	arg1	independent					1110:1120	independent	1110:1120	independent	1110:1120	Interestingly, the production of TNF-α decreased when macrophages were cultured in middle and high concentration extracts independent of LPS.
29538391	3	21	theme	THP-1	380:384	arg1	cells					386:390	THP-1 cells	380:390	THP-1 cells	380:390	Here, we used two inflammatory cell lines i.e. THP-1 cells and THP-1 macrophages, to evaluate the effect of Mg-Nd-Zn-Zr alloy (denoted as JDBM) extracts on cell viability, death modes, cell cycle, phagocytosis, differentiation, migration and inflammatory response.
29538391	5	22	theme	cell	738:741	arg1	apoptosis					743:751	cell apoptosis	738:751	cell apoptosis	738:751	For middle-concentration extract, cell apoptosis and partially impaired cell function were observed.
29538391	3	23	theme	inflammatory	351:362	arg1	lines					369:373	two inflammatory cell lines	347:373	two inflammatory cell lines i.e. THP-1 cells and THP-1 macrophages	347:412	Here, we used two inflammatory cell lines i.e. THP-1 cells and THP-1 macrophages, to evaluate the effect of Mg-Nd-Zn-Zr alloy (denoted as JDBM) extracts on cell viability, death modes, cell cycle, phagocytosis, differentiation, migration and inflammatory response.
29538391	9	24	theme	Mg	1320:1321	arg1	extracts					1329:1336	Mg alloy extracts	1320:1336	Mg alloy extracts	1320:1336	Our results provide new information about the impact of Mg alloy extracts on phenotype of immune cells and the potential mechanism, which should be taken into account prior to clinical applications.
29538391	6	25	theme	macrophages	825:835	arg1	expression					811:820	TNF-α expression	805:820	TNF-α expression of macrophages	805:835	TNF-α expression of macrophages was up-regulated by co-culture with extract in 20% concentration, but was down-regulated in the same concentration in the presence of LPS stimulation.
29538391	3	26	theme	cell	489:492	arg1	viability					494:502	cell viability	489:502	cell viability	489:502	Here, we used two inflammatory cell lines i.e. THP-1 cells and THP-1 macrophages, to evaluate the effect of Mg-Nd-Zn-Zr alloy (denoted as JDBM) extracts on cell viability, death modes, cell cycle, phagocytosis, differentiation, migration and inflammatory response.
29538391	1	27	theme	vascular	176:183	arg1	stents					185:190	vascular stents	176:190	vascular stents	176:190	Biodegradable Mg-based alloys have shown great potential as bone fixation devices or vascular stents.
29538391	1	27	theme	vascular	176:183	arg1	potential					138:146	great potential	132:146	great potential	132:146	Biodegradable Mg-based alloys have shown great potential as bone fixation devices or vascular stents.
29538391	0	28	theme	In	0:1	arg1	study					9:13	In vitro study	0:13	In vitro study of the inflammatory cells	0:39	In vitro study of the inflammatory cells response to biodegradable Mg-based alloy extract.
29538391	9	29	theme	new	1284:1286	arg1	information					1288:1298	new information	1284:1298	new information about the impact of Mg alloy extracts on phenotype of immune cells and the potential mechanism, which should be taken into account prior to clinical applications	1284:1460	Our results provide new information about the impact of Mg alloy extracts on phenotype of immune cells and the potential mechanism, which should be taken into account prior to clinical applications.
29538391	8	30	theme	JDBM	1195:1198	arg1	extracts					1200:1207	JDBM extracts	1195:1207	JDBM extracts	1195:1207	Cell viability was also negatively affected by magnesium ions in JDBM extracts, which was a potential factor affecting cell function.
29538391	8	30	theme	JDBM	1195:1198	arg1	factor					1232:1237	a potential factor	1220:1237	a potential factor affecting cell function	1220:1261	Cell viability was also negatively affected by magnesium ions in JDBM extracts, which was a potential factor affecting cell function.
29538391	3	31	from	effect	431:436	arg1	modes					511:515	death modes	505:515	death modes	505:515	Here, we used two inflammatory cell lines i.e. THP-1 cells and THP-1 macrophages, to evaluate the effect of Mg-Nd-Zn-Zr alloy (denoted as JDBM) extracts on cell viability, death modes, cell cycle, phagocytosis, differentiation, migration and inflammatory response.
29538391	3	31	from	effect	431:436	arg1	differentiation					544:558	differentiation	544:558	differentiation	544:558	Here, we used two inflammatory cell lines i.e. THP-1 cells and THP-1 macrophages, to evaluate the effect of Mg-Nd-Zn-Zr alloy (denoted as JDBM) extracts on cell viability, death modes, cell cycle, phagocytosis, differentiation, migration and inflammatory response.
29538391	3	31	from	effect	431:436	arg1	migration					561:569	migration	561:569	migration	561:569	Here, we used two inflammatory cell lines i.e. THP-1 cells and THP-1 macrophages, to evaluate the effect of Mg-Nd-Zn-Zr alloy (denoted as JDBM) extracts on cell viability, death modes, cell cycle, phagocytosis, differentiation, migration and inflammatory response.
29538391	3	31	from	effect	431:436	arg1	viability					494:502	cell viability	489:502	cell viability	489:502	Here, we used two inflammatory cell lines i.e. THP-1 cells and THP-1 macrophages, to evaluate the effect of Mg-Nd-Zn-Zr alloy (denoted as JDBM) extracts on cell viability, death modes, cell cycle, phagocytosis, differentiation, migration and inflammatory response.
29538391	3	31	from	effect	431:436	arg1	cycle					523:527	cell cycle	518:527	cell cycle	518:527	Here, we used two inflammatory cell lines i.e. THP-1 cells and THP-1 macrophages, to evaluate the effect of Mg-Nd-Zn-Zr alloy (denoted as JDBM) extracts on cell viability, death modes, cell cycle, phagocytosis, differentiation, migration and inflammatory response.
29538391	3	31	from	effect	431:436	arg1	phagocytosis					530:541	phagocytosis	530:541	phagocytosis	530:541	Here, we used two inflammatory cell lines i.e. THP-1 cells and THP-1 macrophages, to evaluate the effect of Mg-Nd-Zn-Zr alloy (denoted as JDBM) extracts on cell viability, death modes, cell cycle, phagocytosis, differentiation, migration and inflammatory response.
29538391	3	31	from	effect	431:436	arg1	response					588:595	inflammatory response	575:595	inflammatory response	575:595	Here, we used two inflammatory cell lines i.e. THP-1 cells and THP-1 macrophages, to evaluate the effect of Mg-Nd-Zn-Zr alloy (denoted as JDBM) extracts on cell viability, death modes, cell cycle, phagocytosis, differentiation, migration and inflammatory response.
29538391	1	32	theme	Biodegradable	91:103	arg1	alloys					114:119	Biodegradable Mg-based alloys	91:119	Biodegradable Mg-based alloys	91:119	Biodegradable Mg-based alloys have shown great potential as bone fixation devices or vascular stents.
29538391	9	33	theme	alloy	1323:1327	arg1	extracts					1329:1336	Mg alloy extracts	1320:1336	Mg alloy extracts	1320:1336	Our results provide new information about the impact of Mg alloy extracts on phenotype of immune cells and the potential mechanism, which should be taken into account prior to clinical applications.
29538391	5	34	theme	cell	776:779	arg1	function					781:788	partially impaired cell function	757:788	partially impaired cell function	757:788	For middle-concentration extract, cell apoptosis and partially impaired cell function were observed.
29538391	5	35	theme	impaired	767:774	arg1	function					781:788	partially impaired cell function	757:788	partially impaired cell function	757:788	For middle-concentration extract, cell apoptosis and partially impaired cell function were observed.
29538391	7	36	theme	TNF-α	1021:1025	arg1	production					1007:1016	the production	1003:1016	the production of TNF-α	1003:1025	Interestingly, the production of TNF-α decreased when macrophages were cultured in middle and high concentration extracts independent of LPS.
29538391	1	37	theme	Mg-based	105:112	arg1	alloys					114:119	Biodegradable Mg-based alloys	91:119	Biodegradable Mg-based alloys	91:119	Biodegradable Mg-based alloys have shown great potential as bone fixation devices or vascular stents.
29538391	3	38	dep	extracts	477:484	arg1	denoted					460:466	denoted	460:466	denoted as JDBM	460:474	Here, we used two inflammatory cell lines i.e. THP-1 cells and THP-1 macrophages, to evaluate the effect of Mg-Nd-Zn-Zr alloy (denoted as JDBM) extracts on cell viability, death modes, cell cycle, phagocytosis, differentiation, migration and inflammatory response.
29538391	0	39	theme	inflammatory	22:33	arg1	cells					35:39	the inflammatory cells	18:39	the inflammatory cells	18:39	In vitro study of the inflammatory cells response to biodegradable Mg-based alloy extract.
29538391	4	40	theme	high-concentration	622:639	arg1	extract					641:647	high-concentration extract	622:647	high-concentration extract	622:647	The results showed that high-concentration extract induced necrosis and complete damage of cell function.
29538391	2	41	theme	body	230:233	arg1	issue					266:270	an important issue to be studied, where the inflammatory cells play a key role	253:330	an important issue to be studied, where the inflammatory cells play a key role	253:330	As implant biomaterials, the foreign body reaction (FBR) is an important issue to be studied, where the inflammatory cells play a key role.
29538391	2	41	theme	body	230:233	arg1	FBR					245:247	FBR	245:247	FBR	245:247	As implant biomaterials, the foreign body reaction (FBR) is an important issue to be studied, where the inflammatory cells play a key role.
29538391	2	41	theme	body	230:233	arg1	reaction					235:242	the foreign body reaction	218:242	the foreign body reaction (FBR)	218:248	As implant biomaterials, the foreign body reaction (FBR) is an important issue to be studied, where the inflammatory cells play a key role.
29538391	8	42	theme	potential	1222:1230	arg1	extracts					1200:1207	JDBM extracts	1195:1207	JDBM extracts	1195:1207	Cell viability was also negatively affected by magnesium ions in JDBM extracts, which was a potential factor affecting cell function.
29538391	8	42	theme	potential	1222:1230	arg1	factor					1232:1237	a potential factor	1220:1237	a potential factor affecting cell function	1220:1261	Cell viability was also negatively affected by magnesium ions in JDBM extracts, which was a potential factor affecting cell function.
29538391	3	43	theme	cell	364:367	arg1	lines					369:373	two inflammatory cell lines	347:373	two inflammatory cell lines i.e. THP-1 cells and THP-1 macrophages	347:412	Here, we used two inflammatory cell lines i.e. THP-1 cells and THP-1 macrophages, to evaluate the effect of Mg-Nd-Zn-Zr alloy (denoted as JDBM) extracts on cell viability, death modes, cell cycle, phagocytosis, differentiation, migration and inflammatory response.
29538391	7	44	theme	independent	1110:1120	arg1	extracts					1101:1108	middle and high concentration extracts	1071:1108	middle and high concentration extracts independent of LPS	1071:1127	Interestingly, the production of TNF-α decreased when macrophages were cultured in middle and high concentration extracts independent of LPS.
29538391	6	45	theme	TNF-α	805:809	arg1	expression					811:820	TNF-α expression	805:820	TNF-α expression of macrophages	805:835	TNF-α expression of macrophages was up-regulated by co-culture with extract in 20% concentration, but was down-regulated in the same concentration in the presence of LPS stimulation.
29538391	2	46	theme	foreign	222:228	arg1	issue					266:270	an important issue to be studied, where the inflammatory cells play a key role	253:330	an important issue to be studied, where the inflammatory cells play a key role	253:330	As implant biomaterials, the foreign body reaction (FBR) is an important issue to be studied, where the inflammatory cells play a key role.
29538391	2	46	theme	foreign	222:228	arg1	FBR					245:247	FBR	245:247	FBR	245:247	As implant biomaterials, the foreign body reaction (FBR) is an important issue to be studied, where the inflammatory cells play a key role.
29538391	2	46	theme	foreign	222:228	arg1	reaction					235:242	the foreign body reaction	218:242	the foreign body reaction (FBR)	218:248	As implant biomaterials, the foreign body reaction (FBR) is an important issue to be studied, where the inflammatory cells play a key role.
29538391	3	47	theme	THP-1	396:400	arg1	macrophages					402:412	THP-1 macrophages	396:412	THP-1 macrophages	396:412	Here, we used two inflammatory cell lines i.e. THP-1 cells and THP-1 macrophages, to evaluate the effect of Mg-Nd-Zn-Zr alloy (denoted as JDBM) extracts on cell viability, death modes, cell cycle, phagocytosis, differentiation, migration and inflammatory response.
29538391	7	48	theme	high	1082:1085	arg1	extracts					1101:1108	middle and high concentration extracts	1071:1108	middle and high concentration extracts independent of LPS	1071:1127	Interestingly, the production of TNF-α decreased when macrophages were cultured in middle and high concentration extracts independent of LPS.
29538391	6	49	theme	stimulation	975:985	arg1	presence					959:966	the presence	955:966	the presence of LPS stimulation	955:985	TNF-α expression of macrophages was up-regulated by co-culture with extract in 20% concentration, but was down-regulated in the same concentration in the presence of LPS stimulation.
29538391	9	50	theme	potential	1375:1383	arg1	mechanism					1385:1393	the potential mechanism	1371:1393	the potential mechanism	1371:1393	Our results provide new information about the impact of Mg alloy extracts on phenotype of immune cells and the potential mechanism, which should be taken into account prior to clinical applications.
29538391	0	51	theme	cells	35:39	arg1	study					9:13	In vitro study	0:13	In vitro study of the inflammatory cells	0:39	In vitro study of the inflammatory cells response to biodegradable Mg-based alloy extract.
29538391	6	52	theme	%	886:886	arg1	concentration					888:900	20% concentration	884:900	20% concentration	884:900	TNF-α expression of macrophages was up-regulated by co-culture with extract in 20% concentration, but was down-regulated in the same concentration in the presence of LPS stimulation.
29538391	3	53	used	used	342:345	arg2	we					339:340	we	339:340	we	339:340	Here, we used two inflammatory cell lines i.e. THP-1 cells and THP-1 macrophages, to evaluate the effect of Mg-Nd-Zn-Zr alloy (denoted as JDBM) extracts on cell viability, death modes, cell cycle, phagocytosis, differentiation, migration and inflammatory response.
29538391	3	54	theme	death	505:509	arg1	modes					511:515	death modes	505:515	death modes	505:515	Here, we used two inflammatory cell lines i.e. THP-1 cells and THP-1 macrophages, to evaluate the effect of Mg-Nd-Zn-Zr alloy (denoted as JDBM) extracts on cell viability, death modes, cell cycle, phagocytosis, differentiation, migration and inflammatory response.
29538391	3	55	theme	Mg-Nd-Zn-Zr	441:451	arg1	extracts					477:484	Mg-Nd-Zn-Zr alloy (denoted as JDBM) extracts	441:484	Mg-Nd-Zn-Zr alloy (denoted as JDBM) extracts	441:484	Here, we used two inflammatory cell lines i.e. THP-1 cells and THP-1 macrophages, to evaluate the effect of Mg-Nd-Zn-Zr alloy (denoted as JDBM) extracts on cell viability, death modes, cell cycle, phagocytosis, differentiation, migration and inflammatory response.
29538391	2	56	theme	inflammatory	297:308	arg1	cells					310:314	the inflammatory cells	293:314	the inflammatory cells	293:314	As implant biomaterials, the foreign body reaction (FBR) is an important issue to be studied, where the inflammatory cells play a key role.
29538391	6	57	theme	20	884:885	arg1	%					886:886	%	886:886	%	886:886	TNF-α expression of macrophages was up-regulated by co-culture with extract in 20% concentration, but was down-regulated in the same concentration in the presence of LPS stimulation.
29538391	2	58	theme	implant	196:202	arg1	biomaterials					204:215	implant biomaterials	196:215	implant biomaterials	196:215	As implant biomaterials, the foreign body reaction (FBR) is an important issue to be studied, where the inflammatory cells play a key role.
29538391	7	59	theme	concentration	1087:1099	arg1	extracts					1101:1108	middle and high concentration extracts	1071:1108	middle and high concentration extracts independent of LPS	1071:1127	Interestingly, the production of TNF-α decreased when macrophages were cultured in middle and high concentration extracts independent of LPS.
29538391	3	60	theme	extracts	477:484	arg1	effect					431:436	the effect	427:436	the effect of Mg-Nd-Zn-Zr alloy (denoted as JDBM) extracts on cell viability, death modes, cell cycle, phagocytosis, differentiation, migration and inflammatory response	427:595	Here, we used two inflammatory cell lines i.e. THP-1 cells and THP-1 macrophages, to evaluate the effect of Mg-Nd-Zn-Zr alloy (denoted as JDBM) extracts on cell viability, death modes, cell cycle, phagocytosis, differentiation, migration and inflammatory response.
29538391	6	61	theme	LPS	971:973	arg1	stimulation					975:985	LPS stimulation	971:985	LPS stimulation	971:985	TNF-α expression of macrophages was up-regulated by co-culture with extract in 20% concentration, but was down-regulated in the same concentration in the presence of LPS stimulation.
29538391	6	62	with	co-culture	857:866	arg1	extract					873:879	extract	873:879	extract	873:879	TNF-α expression of macrophages was up-regulated by co-culture with extract in 20% concentration, but was down-regulated in the same concentration in the presence of LPS stimulation.
29538391	1	63	theme	great	132:136	arg1	devices					165:171	bone fixation devices	151:171	bone fixation devices	151:171	Biodegradable Mg-based alloys have shown great potential as bone fixation devices or vascular stents.
29538391	1	63	theme	great	132:136	arg1	stents					185:190	vascular stents	176:190	vascular stents	176:190	Biodegradable Mg-based alloys have shown great potential as bone fixation devices or vascular stents.
29538391	1	63	theme	great	132:136	arg1	potential					138:146	great potential	132:146	great potential	132:146	Biodegradable Mg-based alloys have shown great potential as bone fixation devices or vascular stents.
29538391	0	64	theme	biodegradable	53:65	arg1	extract					82:88	biodegradable Mg-based alloy extract	53:88	biodegradable Mg-based alloy extract	53:88	In vitro study of the inflammatory cells response to biodegradable Mg-based alloy extract.
29538391	3	65	theme	inflammatory	575:586	arg1	response					588:595	inflammatory response	575:595	inflammatory response	575:595	Here, we used two inflammatory cell lines i.e. THP-1 cells and THP-1 macrophages, to evaluate the effect of Mg-Nd-Zn-Zr alloy (denoted as JDBM) extracts on cell viability, death modes, cell cycle, phagocytosis, differentiation, migration and inflammatory response.
29538391	3	66	theme	cell	518:521	arg1	cycle					523:527	cell cycle	518:527	cell cycle	518:527	Here, we used two inflammatory cell lines i.e. THP-1 cells and THP-1 macrophages, to evaluate the effect of Mg-Nd-Zn-Zr alloy (denoted as JDBM) extracts on cell viability, death modes, cell cycle, phagocytosis, differentiation, migration and inflammatory response.
29538391	3	67	theme	alloy	453:457	arg1	extracts					477:484	Mg-Nd-Zn-Zr alloy (denoted as JDBM) extracts	441:484	Mg-Nd-Zn-Zr alloy (denoted as JDBM) extracts	441:484	Here, we used two inflammatory cell lines i.e. THP-1 cells and THP-1 macrophages, to evaluate the effect of Mg-Nd-Zn-Zr alloy (denoted as JDBM) extracts on cell viability, death modes, cell cycle, phagocytosis, differentiation, migration and inflammatory response.
29538391	9	68	theme	mechanism	1385:1393	arg1	phenotype					1341:1349	phenotype	1341:1349	phenotype of immune cells and the potential mechanism, which should be taken into account prior to clinical applications	1341:1460	Our results provide new information about the impact of Mg alloy extracts on phenotype of immune cells and the potential mechanism, which should be taken into account prior to clinical applications.
29538391	0	69	dep	In	0:1	arg1	vitro					3:7	vitro	3:7	vitro	3:7	In vitro study of the inflammatory cells response to biodegradable Mg-based alloy extract.
29538391	2	70	theme	key	323:325	arg1	role					327:330	a key role	321:330	a key role	321:330	As implant biomaterials, the foreign body reaction (FBR) is an important issue to be studied, where the inflammatory cells play a key role.
29538391	3	71	dep	cells	386:390	arg1	i.e.					375:378	i.e.	375:378	i.e.	375:378	Here, we used two inflammatory cell lines i.e. THP-1 cells and THP-1 macrophages, to evaluate the effect of Mg-Nd-Zn-Zr alloy (denoted as JDBM) extracts on cell viability, death modes, cell cycle, phagocytosis, differentiation, migration and inflammatory response.
31398556	4	0	theme	microbial-associated	779:798	arg1	patterns					810:817	microbial-associated molecular patterns	779:817	microbial-associated molecular patterns	779:817	The model displays the physiological immune tolerance of the intestinal lumen to microbial-associated molecular patterns and can, therefore, be colonised with living microorganisms.
31398556	3	1	theme	organotypic	546:556	arg1	structures					574:583	organotypic microanatomical structures	546:583	organotypic microanatomical structures	546:583	This model resembles organotypic microanatomical structures and includes tissue resident innate immune cells exhibiting features of mucosal macrophages and dendritic cells.
31398556	0	2	theme	functional	85:94	arg1	studies					122:128	functional and microbial interaction studies	85:128	functional and microbial interaction studies	85:128	A three-dimensional immunocompetent intestine-on-chip model as in vitro platform for functional and microbial interaction studies.
31398556	3	3	theme	microanatomical	558:572	arg1	structures					574:583	organotypic microanatomical structures	546:583	organotypic microanatomical structures	546:583	This model resembles organotypic microanatomical structures and includes tissue resident innate immune cells exhibiting features of mucosal macrophages and dendritic cells.
31398556	5	4	theme	albicans-induced	1105:1120	arg1	damage					1129:1134	C. albicans-induced tissue damage	1102:1134	C. albicans-induced tissue damage	1102:1134	Functional studies on microbial interaction between probiotic Lactobacillus rhamnosus and the opportunistic pathogen Candida albicans show that pre-colonization of the intestinal lumen of the model by L. rhamnosus reduces C. albicans-induced tissue damage, lowers its translocation, and limits fungal burden.
31398556	0	5	theme	interaction	110:120	arg1	studies					122:128	functional and microbial interaction studies	85:128	functional and microbial interaction studies	85:128	A three-dimensional immunocompetent intestine-on-chip model as in vitro platform for functional and microbial interaction studies.
31398556	7	6	theme	dysfunction	1504:1514	arg1	mechanisms					1461:1470	microbial pathogenicity mechanisms	1437:1470	microbial pathogenicity mechanisms	1437:1470	The intestinal model allows a detailed characterisation of the immune response, microbial pathogenicity mechanisms, and quantification of cellular dysfunction attributed to alterations in the microbial composition.
31398556	7	6	theme	dysfunction	1504:1514	arg1	quantification					1477:1490	quantification	1477:1490	quantification of cellular dysfunction attributed to alterations in the microbial composition	1477:1569	The intestinal model allows a detailed characterisation of the immune response, microbial pathogenicity mechanisms, and quantification of cellular dysfunction attributed to alterations in the microbial composition.
31398556	7	6	theme	dysfunction	1504:1514	arg1	response					1427:1434	the immune response	1416:1434	the immune response	1416:1434	The intestinal model allows a detailed characterisation of the immune response, microbial pathogenicity mechanisms, and quantification of cellular dysfunction attributed to alterations in the microbial composition.
31398556	1	7	from	gut	179:181	arg1	Alterations					131:141	Alterations	131:141	Alterations of the microbial composition in the gut	131:181	Alterations of the microbial composition in the gut and the concomitant dysregulation of the mucosal immune response are associated with the pathogenesis of opportunistic infections, chronic inflammation, and inflammatory bowel disease.
31398556	1	7	from	gut	179:181	arg1	dysregulation					203:215	the concomitant dysregulation	187:215	the concomitant dysregulation of the mucosal immune response	187:246	Alterations of the microbial composition in the gut and the concomitant dysregulation of the mucosal immune response are associated with the pathogenesis of opportunistic infections, chronic inflammation, and inflammatory bowel disease.
31398556	3	8	theme	resident	605:612	arg1	cells					628:632	tissue resident innate immune cells	598:632	tissue resident innate immune cells exhibiting features of mucosal macrophages and dendritic cells	598:695	This model resembles organotypic microanatomical structures and includes tissue resident innate immune cells exhibiting features of mucosal macrophages and dendritic cells.
31398556	0	9	theme	microbial	100:108	arg1	studies					122:128	functional and microbial interaction studies	85:128	functional and microbial interaction studies	85:128	A three-dimensional immunocompetent intestine-on-chip model as in vitro platform for functional and microbial interaction studies.
31398556	1	10	from	composition	160:170	arg1	gut					179:181	the gut	175:181	the gut	175:181	Alterations of the microbial composition in the gut and the concomitant dysregulation of the mucosal immune response are associated with the pathogenesis of opportunistic infections, chronic inflammation, and inflammatory bowel disease.
31398556	3	11	theme	innate	614:619	arg1	cells					628:632	tissue resident innate immune cells	598:632	tissue resident innate immune cells exhibiting features of mucosal macrophages and dendritic cells	598:695	This model resembles organotypic microanatomical structures and includes tissue resident innate immune cells exhibiting features of mucosal macrophages and dendritic cells.
31398556	2	12	theme	three-dimensional	458:474	arg1	model					495:499	a three-dimensional microphysiological model	456:499	a three-dimensional microphysiological model of the human intestine	456:522	To create a platform for the investigation of the underlying mechanisms, we established a three-dimensional microphysiological model of the human intestine.
31398556	1	13	theme	opportunistic	288:300	arg1	infections					302:311	opportunistic infections	288:311	opportunistic infections	288:311	Alterations of the microbial composition in the gut and the concomitant dysregulation of the mucosal immune response are associated with the pathogenesis of opportunistic infections, chronic inflammation, and inflammatory bowel disease.
31398556	7	14	theme	microbial	1437:1445	arg1	mechanisms					1461:1470	microbial pathogenicity mechanisms	1437:1470	microbial pathogenicity mechanisms	1437:1470	The intestinal model allows a detailed characterisation of the immune response, microbial pathogenicity mechanisms, and quantification of cellular dysfunction attributed to alterations in the microbial composition.
31398556	5	15	theme	microbial	902:910	arg1	interaction					912:922	microbial interaction	902:922	microbial interaction between probiotic Lactobacillus rhamnosus and the opportunistic pathogen Candida albicans	902:1012	Functional studies on microbial interaction between probiotic Lactobacillus rhamnosus and the opportunistic pathogen Candida albicans show that pre-colonization of the intestinal lumen of the model by L. rhamnosus reduces C. albicans-induced tissue damage, lowers its translocation, and limits fungal burden.
31398556	5	16	theme	C.	1102:1103	arg1	damage					1129:1134	C. albicans-induced tissue damage	1102:1134	C. albicans-induced tissue damage	1102:1134	Functional studies on microbial interaction between probiotic Lactobacillus rhamnosus and the opportunistic pathogen Candida albicans show that pre-colonization of the intestinal lumen of the model by L. rhamnosus reduces C. albicans-induced tissue damage, lowers its translocation, and limits fungal burden.
31398556	5	17	theme	Candida	997:1003	arg1	albicans					1005:1012	the opportunistic pathogen Candida albicans	970:1012	the opportunistic pathogen Candida albicans	970:1012	Functional studies on microbial interaction between probiotic Lactobacillus rhamnosus and the opportunistic pathogen Candida albicans show that pre-colonization of the intestinal lumen of the model by L. rhamnosus reduces C. albicans-induced tissue damage, lowers its translocation, and limits fungal burden.
31398556	2	18	theme	underlying	418:427	arg1	mechanisms					429:438	the underlying mechanisms	414:438	the underlying mechanisms	414:438	To create a platform for the investigation of the underlying mechanisms, we established a three-dimensional microphysiological model of the human intestine.
31398556	7	19	theme	quantification	1477:1490	arg1	characterisation					1396:1411	a detailed characterisation	1385:1411	a detailed characterisation of the immune response, microbial pathogenicity mechanisms, and quantification of cellular dysfunction attributed to alterations in the microbial composition	1385:1569	The intestinal model allows a detailed characterisation of the immune response, microbial pathogenicity mechanisms, and quantification of cellular dysfunction attributed to alterations in the microbial composition.
31398556	3	20	theme	tissue	598:603	arg1	cells					628:632	tissue resident innate immune cells	598:632	tissue resident innate immune cells exhibiting features of mucosal macrophages and dendritic cells	598:695	This model resembles organotypic microanatomical structures and includes tissue resident innate immune cells exhibiting features of mucosal macrophages and dendritic cells.
31398556	4	21	theme	immune	735:740	arg1	tolerance					742:750	the physiological immune tolerance	717:750	the physiological immune tolerance of the intestinal lumen to microbial-associated molecular patterns	717:817	The model displays the physiological immune tolerance of the intestinal lumen to microbial-associated molecular patterns and can, therefore, be colonised with living microorganisms.
31398556	0	22	theme	three-dimensional	2:18	arg1	model					54:58	A three-dimensional immunocompetent intestine-on-chip model	0:58	A three-dimensional immunocompetent intestine-on-chip model	0:58	A three-dimensional immunocompetent intestine-on-chip model as in vitro platform for functional and microbial interaction studies.
31398556	6	23	theme	microbial	1209:1217	arg1	interactions					1219:1230	microbial interactions	1209:1230	microbial interactions	1209:1230	We demonstrate that microbial interactions can be efficiently investigated using the in vitro model creating a more physiological and immunocompetent microenvironment.
31398556	6	24	dep	in	1274:1275	arg1	vitro					1277:1281	vitro	1277:1281	vitro	1277:1281	We demonstrate that microbial interactions can be efficiently investigated using the in vitro model creating a more physiological and immunocompetent microenvironment.
31398556	5	25	theme	intestinal	1048:1057	arg1	lumen					1059:1063	the intestinal lumen	1044:1063	the intestinal lumen of the model	1044:1076	Functional studies on microbial interaction between probiotic Lactobacillus rhamnosus and the opportunistic pathogen Candida albicans show that pre-colonization of the intestinal lumen of the model by L. rhamnosus reduces C. albicans-induced tissue damage, lowers its translocation, and limits fungal burden.
31398556	1	26	theme	concomitant	191:201	arg1	dysregulation					203:215	the concomitant dysregulation	187:215	the concomitant dysregulation of the mucosal immune response	187:246	Alterations of the microbial composition in the gut and the concomitant dysregulation of the mucosal immune response are associated with the pathogenesis of opportunistic infections, chronic inflammation, and inflammatory bowel disease.
31398556	2	27	theme	microphysiological	476:493	arg1	model					495:499	a three-dimensional microphysiological model	456:499	a three-dimensional microphysiological model of the human intestine	456:522	To create a platform for the investigation of the underlying mechanisms, we established a three-dimensional microphysiological model of the human intestine.
31398556	1	28	theme	infections	302:311	arg1	pathogenesis					272:283	the pathogenesis	268:283	the pathogenesis of opportunistic infections, chronic inflammation, and inflammatory bowel disease	268:365	Alterations of the microbial composition in the gut and the concomitant dysregulation of the mucosal immune response are associated with the pathogenesis of opportunistic infections, chronic inflammation, and inflammatory bowel disease.
31398556	1	29	from	Alterations	131:141	arg1	gut					179:181	the gut	175:181	the gut	175:181	Alterations of the microbial composition in the gut and the concomitant dysregulation of the mucosal immune response are associated with the pathogenesis of opportunistic infections, chronic inflammation, and inflammatory bowel disease.
31398556	5	30	theme	model	1072:1076	arg1	lumen					1059:1063	the intestinal lumen	1044:1063	the intestinal lumen of the model	1044:1076	Functional studies on microbial interaction between probiotic Lactobacillus rhamnosus and the opportunistic pathogen Candida albicans show that pre-colonization of the intestinal lumen of the model by L. rhamnosus reduces C. albicans-induced tissue damage, lowers its translocation, and limits fungal burden.
31398556	7	31	theme	mechanisms	1461:1470	arg1	characterisation					1396:1411	a detailed characterisation	1385:1411	a detailed characterisation of the immune response, microbial pathogenicity mechanisms, and quantification of cellular dysfunction attributed to alterations in the microbial composition	1385:1569	The intestinal model allows a detailed characterisation of the immune response, microbial pathogenicity mechanisms, and quantification of cellular dysfunction attributed to alterations in the microbial composition.
31398556	7	32	theme	cellular	1495:1502	arg1	dysfunction					1504:1514	cellular dysfunction	1495:1514	cellular dysfunction attributed to alterations in the microbial composition	1495:1569	The intestinal model allows a detailed characterisation of the immune response, microbial pathogenicity mechanisms, and quantification of cellular dysfunction attributed to alterations in the microbial composition.
31398556	0	33	theme	intestine-on-chip	36:52	arg1	model					54:58	A three-dimensional immunocompetent intestine-on-chip model	0:58	A three-dimensional immunocompetent intestine-on-chip model	0:58	A three-dimensional immunocompetent intestine-on-chip model as in vitro platform for functional and microbial interaction studies.
31398556	5	34	dep	Lactobacillus	942:954	arg1	rhamnosus					956:964	Lactobacillus rhamnosus	942:964	Lactobacillus rhamnosus	942:964	Functional studies on microbial interaction between probiotic Lactobacillus rhamnosus and the opportunistic pathogen Candida albicans show that pre-colonization of the intestinal lumen of the model by L. rhamnosus reduces C. albicans-induced tissue damage, lowers its translocation, and limits fungal burden.
31398556	3	35	theme	mucosal	657:663	arg1	macrophages					665:675	mucosal macrophages	657:675	mucosal macrophages	657:675	This model resembles organotypic microanatomical structures and includes tissue resident innate immune cells exhibiting features of mucosal macrophages and dendritic cells.
31398556	3	36	theme	immune	621:626	arg1	cells					628:632	tissue resident innate immune cells	598:632	tissue resident innate immune cells exhibiting features of mucosal macrophages and dendritic cells	598:695	This model resembles organotypic microanatomical structures and includes tissue resident innate immune cells exhibiting features of mucosal macrophages and dendritic cells.
31398556	0	37	theme	immunocompetent	20:34	arg1	model					54:58	A three-dimensional immunocompetent intestine-on-chip model	0:58	A three-dimensional immunocompetent intestine-on-chip model	0:58	A three-dimensional immunocompetent intestine-on-chip model as in vitro platform for functional and microbial interaction studies.
31398556	1	38	theme	chronic	314:320	arg1	inflammation					322:333	chronic inflammation	314:333	chronic inflammation	314:333	Alterations of the microbial composition in the gut and the concomitant dysregulation of the mucosal immune response are associated with the pathogenesis of opportunistic infections, chronic inflammation, and inflammatory bowel disease.
31398556	6	39	theme	physiological	1305:1317	arg1	microenvironment					1339:1354	a more physiological and immunocompetent microenvironment	1298:1354	a more physiological and immunocompetent microenvironment	1298:1354	We demonstrate that microbial interactions can be efficiently investigated using the in vitro model creating a more physiological and immunocompetent microenvironment.
31398556	4	40	theme	lumen	770:774	arg1	tolerance					742:750	the physiological immune tolerance	717:750	the physiological immune tolerance of the intestinal lumen to microbial-associated molecular patterns	717:817	The model displays the physiological immune tolerance of the intestinal lumen to microbial-associated molecular patterns and can, therefore, be colonised with living microorganisms.
31398556	2	41	theme	intestine	514:522	arg1	model					495:499	a three-dimensional microphysiological model	456:499	a three-dimensional microphysiological model of the human intestine	456:522	To create a platform for the investigation of the underlying mechanisms, we established a three-dimensional microphysiological model of the human intestine.
31398556	1	42	theme	inflammation	322:333	arg1	pathogenesis					272:283	the pathogenesis	268:283	the pathogenesis of opportunistic infections, chronic inflammation, and inflammatory bowel disease	268:365	Alterations of the microbial composition in the gut and the concomitant dysregulation of the mucosal immune response are associated with the pathogenesis of opportunistic infections, chronic inflammation, and inflammatory bowel disease.
31398556	7	43	from	alterations	1530:1540	arg1	composition					1559:1569	the microbial composition	1545:1569	the microbial composition	1545:1569	The intestinal model allows a detailed characterisation of the immune response, microbial pathogenicity mechanisms, and quantification of cellular dysfunction attributed to alterations in the microbial composition.
31398556	4	44	theme	physiological	721:733	arg1	tolerance					742:750	the physiological immune tolerance	717:750	the physiological immune tolerance of the intestinal lumen to microbial-associated molecular patterns	717:817	The model displays the physiological immune tolerance of the intestinal lumen to microbial-associated molecular patterns and can, therefore, be colonised with living microorganisms.
31398556	3	45	theme	macrophages	665:675	arg1	features					645:652	features	645:652	features of mucosal macrophages and dendritic cells	645:695	This model resembles organotypic microanatomical structures and includes tissue resident innate immune cells exhibiting features of mucosal macrophages and dendritic cells.
31398556	1	46	theme	mucosal	224:230	arg1	response					239:246	the mucosal immune response	220:246	the mucosal immune response	220:246	Alterations of the microbial composition in the gut and the concomitant dysregulation of the mucosal immune response are associated with the pathogenesis of opportunistic infections, chronic inflammation, and inflammatory bowel disease.
31398556	2	47	theme	human	508:512	arg1	intestine					514:522	the human intestine	504:522	the human intestine	504:522	To create a platform for the investigation of the underlying mechanisms, we established a three-dimensional microphysiological model of the human intestine.
31398556	5	48	theme	Functional	880:889	arg1	studies					891:897	Functional studies	880:897	Functional studies on microbial interaction between probiotic Lactobacillus rhamnosus and the opportunistic pathogen Candida albicans	880:1012	Functional studies on microbial interaction between probiotic Lactobacillus rhamnosus and the opportunistic pathogen Candida albicans show that pre-colonization of the intestinal lumen of the model by L. rhamnosus reduces C. albicans-induced tissue damage, lowers its translocation, and limits fungal burden.
31398556	0	49	dep	in	63:64	arg1	vitro					66:70	vitro	66:70	vitro	66:70	A three-dimensional immunocompetent intestine-on-chip model as in vitro platform for functional and microbial interaction studies.
31398556	1	50	theme	immune	232:237	arg1	response					239:246	the mucosal immune response	220:246	the mucosal immune response	220:246	Alterations of the microbial composition in the gut and the concomitant dysregulation of the mucosal immune response are associated with the pathogenesis of opportunistic infections, chronic inflammation, and inflammatory bowel disease.
31398556	7	51	theme	microbial	1549:1557	arg1	composition					1559:1569	the microbial composition	1545:1569	the microbial composition	1545:1569	The intestinal model allows a detailed characterisation of the immune response, microbial pathogenicity mechanisms, and quantification of cellular dysfunction attributed to alterations in the microbial composition.
31398556	6	52	theme	in	1274:1275	arg1	model					1283:1287	the in vitro model	1270:1287	the in vitro model creating a more physiological and immunocompetent microenvironment	1270:1354	We demonstrate that microbial interactions can be efficiently investigated using the in vitro model creating a more physiological and immunocompetent microenvironment.
31398556	0	53	theme	in	63:64	arg1	platform					72:79	in vitro platform	63:79	in vitro platform for functional and microbial interaction studies	63:128	A three-dimensional immunocompetent intestine-on-chip model as in vitro platform for functional and microbial interaction studies.
31398556	1	54	theme	inflammatory	340:351	arg1	disease					359:365	inflammatory bowel disease	340:365	inflammatory bowel disease	340:365	Alterations of the microbial composition in the gut and the concomitant dysregulation of the mucosal immune response are associated with the pathogenesis of opportunistic infections, chronic inflammation, and inflammatory bowel disease.
31398556	5	55	from	studies	891:897	arg1	interaction					912:922	microbial interaction	902:922	microbial interaction between probiotic Lactobacillus rhamnosus and the opportunistic pathogen Candida albicans	902:1012	Functional studies on microbial interaction between probiotic Lactobacillus rhamnosus and the opportunistic pathogen Candida albicans show that pre-colonization of the intestinal lumen of the model by L. rhamnosus reduces C. albicans-induced tissue damage, lowers its translocation, and limits fungal burden.
31398556	0	56	dep	model	54:58	arg1	platform					72:79	in vitro platform	63:79	in vitro platform for functional and microbial interaction studies	63:128	A three-dimensional immunocompetent intestine-on-chip model as in vitro platform for functional and microbial interaction studies.
31398556	4	57	theme	molecular	800:808	arg1	patterns					810:817	microbial-associated molecular patterns	779:817	microbial-associated molecular patterns	779:817	The model displays the physiological immune tolerance of the intestinal lumen to microbial-associated molecular patterns and can, therefore, be colonised with living microorganisms.
31398556	1	58	theme	bowel	353:357	arg1	disease					359:365	inflammatory bowel disease	340:365	inflammatory bowel disease	340:365	Alterations of the microbial composition in the gut and the concomitant dysregulation of the mucosal immune response are associated with the pathogenesis of opportunistic infections, chronic inflammation, and inflammatory bowel disease.
31398556	5	59	theme	fungal	1174:1179	arg1	burden					1181:1186	fungal burden	1174:1186	fungal burden	1174:1186	Functional studies on microbial interaction between probiotic Lactobacillus rhamnosus and the opportunistic pathogen Candida albicans show that pre-colonization of the intestinal lumen of the model by L. rhamnosus reduces C. albicans-induced tissue damage, lowers its translocation, and limits fungal burden.
31398556	5	60	theme	tissue	1122:1127	arg1	damage					1129:1134	C. albicans-induced tissue damage	1102:1134	C. albicans-induced tissue damage	1102:1134	Functional studies on microbial interaction between probiotic Lactobacillus rhamnosus and the opportunistic pathogen Candida albicans show that pre-colonization of the intestinal lumen of the model by L. rhamnosus reduces C. albicans-induced tissue damage, lowers its translocation, and limits fungal burden.
31398556	7	61	theme	immune	1420:1425	arg1	response					1427:1434	the immune response	1416:1434	the immune response	1416:1434	The intestinal model allows a detailed characterisation of the immune response, microbial pathogenicity mechanisms, and quantification of cellular dysfunction attributed to alterations in the microbial composition.
31398556	5	62	theme	lumen	1059:1063	arg1	pre-colonization					1024:1039	pre-colonization	1024:1039	pre-colonization of the intestinal lumen of the model by L. rhamnosus	1024:1092	Functional studies on microbial interaction between probiotic Lactobacillus rhamnosus and the opportunistic pathogen Candida albicans show that pre-colonization of the intestinal lumen of the model by L. rhamnosus reduces C. albicans-induced tissue damage, lowers its translocation, and limits fungal burden.
31398556	1	63	theme	response	239:246	arg1	Alterations					131:141	Alterations	131:141	Alterations of the microbial composition in the gut	131:181	Alterations of the microbial composition in the gut and the concomitant dysregulation of the mucosal immune response are associated with the pathogenesis of opportunistic infections, chronic inflammation, and inflammatory bowel disease.
31398556	1	63	theme	response	239:246	arg1	dysregulation					203:215	the concomitant dysregulation	187:215	the concomitant dysregulation of the mucosal immune response	187:246	Alterations of the microbial composition in the gut and the concomitant dysregulation of the mucosal immune response are associated with the pathogenesis of opportunistic infections, chronic inflammation, and inflammatory bowel disease.
31398556	4	64	theme	living	857:862	arg1	microorganisms					864:877	living microorganisms	857:877	living microorganisms	857:877	The model displays the physiological immune tolerance of the intestinal lumen to microbial-associated molecular patterns and can, therefore, be colonised with living microorganisms.
31398556	7	65	theme	pathogenicity	1447:1459	arg1	mechanisms					1461:1470	microbial pathogenicity mechanisms	1437:1470	microbial pathogenicity mechanisms	1437:1470	The intestinal model allows a detailed characterisation of the immune response, microbial pathogenicity mechanisms, and quantification of cellular dysfunction attributed to alterations in the microbial composition.
31398556	6	66	theme	immunocompetent	1323:1337	arg1	microenvironment					1339:1354	a more physiological and immunocompetent microenvironment	1298:1354	a more physiological and immunocompetent microenvironment	1298:1354	We demonstrate that microbial interactions can be efficiently investigated using the in vitro model creating a more physiological and immunocompetent microenvironment.
31398556	5	67	theme	opportunistic	974:986	arg1	albicans					1005:1012	the opportunistic pathogen Candida albicans	970:1012	the opportunistic pathogen Candida albicans	970:1012	Functional studies on microbial interaction between probiotic Lactobacillus rhamnosus and the opportunistic pathogen Candida albicans show that pre-colonization of the intestinal lumen of the model by L. rhamnosus reduces C. albicans-induced tissue damage, lowers its translocation, and limits fungal burden.
31398556	1	68	theme	microbial	150:158	arg1	composition					160:170	the microbial composition	146:170	the microbial composition in the gut	146:181	Alterations of the microbial composition in the gut and the concomitant dysregulation of the mucosal immune response are associated with the pathogenesis of opportunistic infections, chronic inflammation, and inflammatory bowel disease.
31398556	7	69	theme	response	1427:1434	arg1	characterisation					1396:1411	a detailed characterisation	1385:1411	a detailed characterisation of the immune response, microbial pathogenicity mechanisms, and quantification of cellular dysfunction attributed to alterations in the microbial composition	1385:1569	The intestinal model allows a detailed characterisation of the immune response, microbial pathogenicity mechanisms, and quantification of cellular dysfunction attributed to alterations in the microbial composition.
31398556	2	70	theme	mechanisms	429:438	arg1	investigation					397:409	the investigation	393:409	the investigation of the underlying mechanisms	393:438	To create a platform for the investigation of the underlying mechanisms, we established a three-dimensional microphysiological model of the human intestine.
31398556	3	71	theme	dendritic	681:689	arg1	cells					691:695	dendritic cells	681:695	dendritic cells	681:695	This model resembles organotypic microanatomical structures and includes tissue resident innate immune cells exhibiting features of mucosal macrophages and dendritic cells.
31398556	1	72	from	dysregulation	203:215	arg1	gut					179:181	the gut	175:181	the gut	175:181	Alterations of the microbial composition in the gut and the concomitant dysregulation of the mucosal immune response are associated with the pathogenesis of opportunistic infections, chronic inflammation, and inflammatory bowel disease.
31398556	1	73	theme	composition	160:170	arg1	Alterations					131:141	Alterations	131:141	Alterations of the microbial composition in the gut	131:181	Alterations of the microbial composition in the gut and the concomitant dysregulation of the mucosal immune response are associated with the pathogenesis of opportunistic infections, chronic inflammation, and inflammatory bowel disease.
31398556	1	73	theme	composition	160:170	arg1	dysregulation					203:215	the concomitant dysregulation	187:215	the concomitant dysregulation of the mucosal immune response	187:246	Alterations of the microbial composition in the gut and the concomitant dysregulation of the mucosal immune response are associated with the pathogenesis of opportunistic infections, chronic inflammation, and inflammatory bowel disease.
31398556	5	74	theme	pathogen	988:995	arg1	albicans					1005:1012	the opportunistic pathogen Candida albicans	970:1012	the opportunistic pathogen Candida albicans	970:1012	Functional studies on microbial interaction between probiotic Lactobacillus rhamnosus and the opportunistic pathogen Candida albicans show that pre-colonization of the intestinal lumen of the model by L. rhamnosus reduces C. albicans-induced tissue damage, lowers its translocation, and limits fungal burden.
31398556	7	75	theme	detailed	1387:1394	arg1	characterisation					1396:1411	a detailed characterisation	1385:1411	a detailed characterisation of the immune response, microbial pathogenicity mechanisms, and quantification of cellular dysfunction attributed to alterations in the microbial composition	1385:1569	The intestinal model allows a detailed characterisation of the immune response, microbial pathogenicity mechanisms, and quantification of cellular dysfunction attributed to alterations in the microbial composition.
31398556	1	76	theme	disease	359:365	arg1	pathogenesis					272:283	the pathogenesis	268:283	the pathogenesis of opportunistic infections, chronic inflammation, and inflammatory bowel disease	268:365	Alterations of the microbial composition in the gut and the concomitant dysregulation of the mucosal immune response are associated with the pathogenesis of opportunistic infections, chronic inflammation, and inflammatory bowel disease.
31398556	3	77	theme	cells	691:695	arg1	features					645:652	features	645:652	features of mucosal macrophages and dendritic cells	645:695	This model resembles organotypic microanatomical structures and includes tissue resident innate immune cells exhibiting features of mucosal macrophages and dendritic cells.
31398556	4	78	theme	intestinal	759:768	arg1	lumen					770:774	the intestinal lumen	755:774	the intestinal lumen	755:774	The model displays the physiological immune tolerance of the intestinal lumen to microbial-associated molecular patterns and can, therefore, be colonised with living microorganisms.
31398556	7	79	theme	intestinal	1361:1370	arg1	model					1372:1376	The intestinal model	1357:1376	The intestinal model	1357:1376	The intestinal model allows a detailed characterisation of the immune response, microbial pathogenicity mechanisms, and quantification of cellular dysfunction attributed to alterations in the microbial composition.
29879442	10	0	theme	brain	1876:1880	arg1	content					1882:1888	brain content	1876:1888	brain content in n-3 PUFA and cytokine production in MIA-exposed fetuses	1876:1947	We also showed a strong negative correlation between brain content in n-3 PUFA and cytokine production in MIA-exposed fetuses.
29879442	7	1	theme	present	1171:1177	arg1	study					1179:1183	The present study	1167:1183	The present study	1167:1183	The present study aimed at providing insight into the effects of n-3 PUFAs on the consequences of MIA on brain development.
29879442	9	2	theme	offspring	1631:1639	arg1	brain					1641:1645	the fetal and adult offspring brain	1611:1645	the fetal and adult offspring brain	1611:1645	Based on a lipopolysaccharide (LPS) model of MIA (LPS injection at embryonic day 17), we showed that n-3 PUFA deficiency 1) alters fatty acid composition of the fetal and adult offspring brain; 2) exacerbates maternal and fetal inflammatory processes with no significant alteration of microglia phenotype, and 3) induces spatial memory deficits in the adult offspring.
29879442	10	3	theme	cytokine	1906:1913	arg1	production					1915:1924	cytokine production	1906:1924	cytokine production	1906:1924	We also showed a strong negative correlation between brain content in n-3 PUFA and cytokine production in MIA-exposed fetuses.
29879442	3	4	theme	fetal	608:612	arg1	compartments					614:625	the maternal and fetal compartments	591:625	the maternal and fetal compartments	591:625	Accumulating evidence point out that proinflammatory cytokines produced both in the maternal and fetal compartments are responsible for social, cognitive and emotional behavioral deficits in the offspring.
29879442	1	5	theme	neurodevelopmental	267:284	arg1	disorders					286:294	neurodevelopmental disorders	267:294	neurodevelopmental disorders	267:294	Maternal immune activation (MIA) is a common environmental insult on the developing brain and represents a risk factor for neurodevelopmental disorders.
29879442	9	6	from	day	1531:1533	arg1	injection					1508:1516	LPS injection	1504:1516	LPS injection at embryonic day 17	1504:1536	Based on a lipopolysaccharide (LPS) model of MIA (LPS injection at embryonic day 17), we showed that n-3 PUFA deficiency 1) alters fatty acid composition of the fetal and adult offspring brain; 2) exacerbates maternal and fetal inflammatory processes with no significant alteration of microglia phenotype, and 3) induces spatial memory deficits in the adult offspring.
29879442	11	7	theme	disorders	2250:2258	arg1	apparition					2217:2226	the apparition	2213:2226	the apparition of neurodevelopmental disorders	2213:2258	Overall, our study is the first to address the deleterious effects of n-3 PUFA deficiency on brain lipid composition, inflammation and memory performances in MIA-exposed animals and indicates that it should be considered as a potent environmental risk factor for the apparition of neurodevelopmental disorders.
29879442	3	8	theme	maternal	595:602	arg1	compartments					614:625	the maternal and fetal compartments	591:625	the maternal and fetal compartments	591:625	Accumulating evidence point out that proinflammatory cytokines produced both in the maternal and fetal compartments are responsible for social, cognitive and emotional behavioral deficits in the offspring.
29879442	9	9	theme	lipopolysaccharide	1465:1482	arg1	model					1490:1494	a lipopolysaccharide (LPS) model	1463:1494	a lipopolysaccharide (LPS) model of MIA (LPS injection at embryonic day 17)	1463:1537	Based on a lipopolysaccharide (LPS) model of MIA (LPS injection at embryonic day 17), we showed that n-3 PUFA deficiency 1) alters fatty acid composition of the fetal and adult offspring brain; 2) exacerbates maternal and fetal inflammatory processes with no significant alteration of microglia phenotype, and 3) induces spatial memory deficits in the adult offspring.
29879442	6	10	theme	series	952:957	arg1	PUFAs					935:939	PUFAs	935:939	PUFAs of the n-3 series ('n-3 PUFAs', also known as omega-3)	935:994	PUFAs of the n-3 series ('n-3 PUFAs', also known as omega-3) exhibit anti-inflammatory/pro-resolution properties and promote immune functions, while PUFAs of the n-6 series ('n-6 PUFAs' or omega-6) favor pro-inflammatory responses.
29879442	11	11	theme	memory	2085:2090	arg1	performances					2092:2103	memory performances	2085:2103	memory performances	2085:2103	Overall, our study is the first to address the deleterious effects of n-3 PUFA deficiency on brain lipid composition, inflammation and memory performances in MIA-exposed animals and indicates that it should be considered as a potent environmental risk factor for the apparition of neurodevelopmental disorders.
29879442	11	12	theme	deleterious	1997:2007	arg1	effects					2009:2015	the deleterious effects	1993:2015	the deleterious effects of n-3 PUFA deficiency on brain lipid composition, inflammation and memory performances in MIA-exposed animals	1993:2126	Overall, our study is the first to address the deleterious effects of n-3 PUFA deficiency on brain lipid composition, inflammation and memory performances in MIA-exposed animals and indicates that it should be considered as a potent environmental risk factor for the apparition of neurodevelopmental disorders.
29879442	1	13	theme	environmental	189:201	arg1	activation					160:169	Maternal immune activation	144:169	Maternal immune activation (MIA)	144:175	Maternal immune activation (MIA) is a common environmental insult on the developing brain and represents a risk factor for neurodevelopmental disorders.
29879442	1	13	theme	environmental	189:201	arg1	insult					203:208	a common environmental insult	180:208	a common environmental insult on the developing brain	180:232	Maternal immune activation (MIA) is a common environmental insult on the developing brain and represents a risk factor for neurodevelopmental disorders.
29879442	9	14	theme	maternal	1663:1670	arg1	processes					1695:1703	maternal and fetal inflammatory processes	1663:1703	maternal and fetal inflammatory processes	1663:1703	Based on a lipopolysaccharide (LPS) model of MIA (LPS injection at embryonic day 17), we showed that n-3 PUFA deficiency 1) alters fatty acid composition of the fetal and adult offspring brain; 2) exacerbates maternal and fetal inflammatory processes with no significant alteration of microglia phenotype, and 3) induces spatial memory deficits in the adult offspring.
29879442	5	15	theme	inflammatory	912:923	arg1	response					925:932	the immune and inflammatory response	897:932	the immune and inflammatory response	897:932	PUFAs and their bioactive derivatives can promote or inhibit many aspects of the immune and inflammatory response.
29879442	10	16	from	PUFA	1897:1900	arg1	fetuses					1941:1947	MIA-exposed fetuses	1929:1947	MIA-exposed fetuses	1929:1947	We also showed a strong negative correlation between brain content in n-3 PUFA and cytokine production in MIA-exposed fetuses.
29879442	9	17	theme	fetal	1676:1680	arg1	processes					1695:1703	maternal and fetal inflammatory processes	1663:1703	maternal and fetal inflammatory processes	1663:1703	Based on a lipopolysaccharide (LPS) model of MIA (LPS injection at embryonic day 17), we showed that n-3 PUFA deficiency 1) alters fatty acid composition of the fetal and adult offspring brain; 2) exacerbates maternal and fetal inflammatory processes with no significant alteration of microglia phenotype, and 3) induces spatial memory deficits in the adult offspring.
29879442	4	18	theme	Polyunsaturated	717:731	arg1	acids					739:743	Polyunsaturated fatty acids	717:743	Polyunsaturated fatty acids (PUFAs)	717:751	Polyunsaturated fatty acids (PUFAs) are essential fatty acids with potent immunomodulatory activities.
29879442	4	18	theme	Polyunsaturated	717:731	arg1	acids					773:777	essential fatty acids	757:777	essential fatty acids with potent immunomodulatory activities	757:817	Polyunsaturated fatty acids (PUFAs) are essential fatty acids with potent immunomodulatory activities.
29879442	4	18	theme	Polyunsaturated	717:731	arg1	PUFAs					746:750	PUFAs	746:750	PUFAs	746:750	Polyunsaturated fatty acids (PUFAs) are essential fatty acids with potent immunomodulatory activities.
29879442	11	19	from	composition	2055:2065	arg1	animals					2120:2126	MIA-exposed animals	2108:2126	MIA-exposed animals	2108:2126	Overall, our study is the first to address the deleterious effects of n-3 PUFA deficiency on brain lipid composition, inflammation and memory performances in MIA-exposed animals and indicates that it should be considered as a potent environmental risk factor for the apparition of neurodevelopmental disorders.
29879442	1	20	theme	developing	217:226	arg1	brain					228:232	the developing brain	213:232	the developing brain	213:232	Maternal immune activation (MIA) is a common environmental insult on the developing brain and represents a risk factor for neurodevelopmental disorders.
29879442	11	21	theme	brain	2043:2047	arg1	composition					2055:2065	brain lipid composition	2043:2065	brain lipid composition	2043:2065	Overall, our study is the first to address the deleterious effects of n-3 PUFA deficiency on brain lipid composition, inflammation and memory performances in MIA-exposed animals and indicates that it should be considered as a potent environmental risk factor for the apparition of neurodevelopmental disorders.
29879442	2	22	dep	in	314:315	arg1	utero					317:321	utero	317:321	utero	317:321	Animal models of in utero inflammation further revealed a causal link between maternal inflammatory activation during pregnancy and behavioural impairment relevant to neurodevelopmental disorders in the offspring.
29879442	11	23	from	inflammation	2068:2079	arg1	animals					2120:2126	MIA-exposed animals	2108:2126	MIA-exposed animals	2108:2126	Overall, our study is the first to address the deleterious effects of n-3 PUFA deficiency on brain lipid composition, inflammation and memory performances in MIA-exposed animals and indicates that it should be considered as a potent environmental risk factor for the apparition of neurodevelopmental disorders.
29879442	1	24	from	insult	203:208	arg1	brain					228:232	the developing brain	213:232	the developing brain	213:232	Maternal immune activation (MIA) is a common environmental insult on the developing brain and represents a risk factor for neurodevelopmental disorders.
29879442	9	25	theme	LPS	1504:1506	arg1	injection					1508:1516	LPS injection	1504:1516	LPS injection at embryonic day 17	1504:1536	Based on a lipopolysaccharide (LPS) model of MIA (LPS injection at embryonic day 17), we showed that n-3 PUFA deficiency 1) alters fatty acid composition of the fetal and adult offspring brain; 2) exacerbates maternal and fetal inflammatory processes with no significant alteration of microglia phenotype, and 3) induces spatial memory deficits in the adult offspring.
29879442	11	26	theme	deficiency	2029:2038	arg1	effects					2009:2015	the deleterious effects	1993:2015	the deleterious effects of n-3 PUFA deficiency on brain lipid composition, inflammation and memory performances in MIA-exposed animals	1993:2126	Overall, our study is the first to address the deleterious effects of n-3 PUFA deficiency on brain lipid composition, inflammation and memory performances in MIA-exposed animals and indicates that it should be considered as a potent environmental risk factor for the apparition of neurodevelopmental disorders.
29879442	7	27	from	consequences	1249:1260	arg1	development					1278:1288	brain development	1272:1288	brain development	1272:1288	The present study aimed at providing insight into the effects of n-3 PUFAs on the consequences of MIA on brain development.
29879442	4	28	theme	immunomodulatory	791:806	arg1	activities					808:817	potent immunomodulatory activities	784:817	potent immunomodulatory activities	784:817	Polyunsaturated fatty acids (PUFAs) are essential fatty acids with potent immunomodulatory activities.
29879442	1	29	theme	immune	153:158	arg1	MIA					172:174	MIA	172:174	MIA	172:174	Maternal immune activation (MIA) is a common environmental insult on the developing brain and represents a risk factor for neurodevelopmental disorders.
29879442	1	29	theme	immune	153:158	arg1	insult					203:208	a common environmental insult	180:208	a common environmental insult on the developing brain	180:232	Maternal immune activation (MIA) is a common environmental insult on the developing brain and represents a risk factor for neurodevelopmental disorders.
29879442	1	29	theme	immune	153:158	arg1	activation					160:169	Maternal immune activation	144:169	Maternal immune activation (MIA)	144:175	Maternal immune activation (MIA) is a common environmental insult on the developing brain and represents a risk factor for neurodevelopmental disorders.
29879442	11	30	theme	potent	2176:2181	arg1	factor					2202:2207	a potent environmental risk factor	2174:2207	a potent environmental risk factor for the apparition of neurodevelopmental disorders	2174:2258	Overall, our study is the first to address the deleterious effects of n-3 PUFA deficiency on brain lipid composition, inflammation and memory performances in MIA-exposed animals and indicates that it should be considered as a potent environmental risk factor for the apparition of neurodevelopmental disorders.
29879442	11	30	theme	potent	2176:2181	arg1	it					2147:2148	it	2147:2148	it	2147:2148	Overall, our study is the first to address the deleterious effects of n-3 PUFA deficiency on brain lipid composition, inflammation and memory performances in MIA-exposed animals and indicates that it should be considered as a potent environmental risk factor for the apparition of neurodevelopmental disorders.
29879442	5	31	theme	bioactive	836:844	arg1	derivatives					846:856	their bioactive derivatives	830:856	their bioactive derivatives	830:856	PUFAs and their bioactive derivatives can promote or inhibit many aspects of the immune and inflammatory response.
29879442	3	32	theme	behavioral	679:688	arg1	deficits					690:697	social, cognitive and emotional behavioral deficits	647:697	social, cognitive and emotional behavioral deficits in the offspring	647:714	Accumulating evidence point out that proinflammatory cytokines produced both in the maternal and fetal compartments are responsible for social, cognitive and emotional behavioral deficits in the offspring.
29879442	9	33	theme	microglia	1739:1747	arg1	phenotype					1749:1757	microglia phenotype	1739:1757	microglia phenotype	1739:1757	Based on a lipopolysaccharide (LPS) model of MIA (LPS injection at embryonic day 17), we showed that n-3 PUFA deficiency 1) alters fatty acid composition of the fetal and adult offspring brain; 2) exacerbates maternal and fetal inflammatory processes with no significant alteration of microglia phenotype, and 3) induces spatial memory deficits in the adult offspring.
29879442	4	34	theme	fatty	767:771	arg1	acids					739:743	Polyunsaturated fatty acids	717:743	Polyunsaturated fatty acids (PUFAs)	717:751	Polyunsaturated fatty acids (PUFAs) are essential fatty acids with potent immunomodulatory activities.
29879442	4	34	theme	fatty	767:771	arg1	acids					773:777	essential fatty acids	757:777	essential fatty acids with potent immunomodulatory activities	757:817	Polyunsaturated fatty acids (PUFAs) are essential fatty acids with potent immunomodulatory activities.
29879442	11	35	from	effects	2009:2015	arg1	inflammation					2068:2079	inflammation	2068:2079	inflammation	2068:2079	Overall, our study is the first to address the deleterious effects of n-3 PUFA deficiency on brain lipid composition, inflammation and memory performances in MIA-exposed animals and indicates that it should be considered as a potent environmental risk factor for the apparition of neurodevelopmental disorders.
29879442	11	35	from	effects	2009:2015	arg1	performances					2092:2103	memory performances	2085:2103	memory performances	2085:2103	Overall, our study is the first to address the deleterious effects of n-3 PUFA deficiency on brain lipid composition, inflammation and memory performances in MIA-exposed animals and indicates that it should be considered as a potent environmental risk factor for the apparition of neurodevelopmental disorders.
29879442	11	35	from	effects	2009:2015	arg1	composition					2055:2065	brain lipid composition	2043:2065	brain lipid composition	2043:2065	Overall, our study is the first to address the deleterious effects of n-3 PUFA deficiency on brain lipid composition, inflammation and memory performances in MIA-exposed animals and indicates that it should be considered as a potent environmental risk factor for the apparition of neurodevelopmental disorders.
29879442	10	36	from	production	1915:1924	arg1	fetuses					1941:1947	MIA-exposed fetuses	1929:1947	MIA-exposed fetuses	1929:1947	We also showed a strong negative correlation between brain content in n-3 PUFA and cytokine production in MIA-exposed fetuses.
29879442	6	37	dep	series	1101:1106	arg1	PUFAs					1114:1118	n-6 PUFAs	1110:1118	n-6 PUFAs	1110:1118	PUFAs of the n-3 series ('n-3 PUFAs', also known as omega-3) exhibit anti-inflammatory/pro-resolution properties and promote immune functions, while PUFAs of the n-6 series ('n-6 PUFAs' or omega-6) favor pro-inflammatory responses.
29879442	6	37	dep	series	1101:1106	arg1	omega-6					1124:1130	omega-6	1124:1130	omega-6	1124:1130	PUFAs of the n-3 series ('n-3 PUFAs', also known as omega-3) exhibit anti-inflammatory/pro-resolution properties and promote immune functions, while PUFAs of the n-6 series ('n-6 PUFAs' or omega-6) favor pro-inflammatory responses.
29879442	2	38	theme	Animal	297:302	arg1	models					304:309	Animal models	297:309	Animal models of in utero inflammation	297:334	Animal models of in utero inflammation further revealed a causal link between maternal inflammatory activation during pregnancy and behavioural impairment relevant to neurodevelopmental disorders in the offspring.
29879442	8	39	theme	n-3	1327:1329	arg1	PUFAs					1331:1335	n-3 PUFAs	1327:1335	n-3 PUFAs	1327:1335	We hypothesized that a reduction in n-3 PUFAs exacerbates both maternal and fetal inflammatory responses to MIA and later-life defects in memory in the offspring.
29879442	11	40	theme	risk	2197:2200	arg1	factor					2202:2207	a potent environmental risk factor	2174:2207	a potent environmental risk factor for the apparition of neurodevelopmental disorders	2174:2258	Overall, our study is the first to address the deleterious effects of n-3 PUFA deficiency on brain lipid composition, inflammation and memory performances in MIA-exposed animals and indicates that it should be considered as a potent environmental risk factor for the apparition of neurodevelopmental disorders.
29879442	11	40	theme	risk	2197:2200	arg1	it					2147:2148	it	2147:2148	it	2147:2148	Overall, our study is the first to address the deleterious effects of n-3 PUFA deficiency on brain lipid composition, inflammation and memory performances in MIA-exposed animals and indicates that it should be considered as a potent environmental risk factor for the apparition of neurodevelopmental disorders.
29879442	7	41	theme	n-3	1232:1234	arg1	PUFAs					1236:1240	n-3 PUFAs	1232:1240	n-3 PUFAs	1232:1240	The present study aimed at providing insight into the effects of n-3 PUFAs on the consequences of MIA on brain development.
29879442	7	42	from	effects	1221:1227	arg1	consequences					1249:1260	the consequences	1245:1260	the consequences of MIA on brain development	1245:1288	The present study aimed at providing insight into the effects of n-3 PUFAs on the consequences of MIA on brain development.
29879442	0	43	theme	Dietary	0:6	arg1	deficiency					16:25	Dietary omega-3 deficiency	0:25	Dietary omega-3 deficiency	0:25	Dietary omega-3 deficiency exacerbates inflammation and reveals spatial memory deficits in mice exposed to lipopolysaccharide during gestation.
29879442	9	44	theme	PUFA	1559:1562	arg1	deficiency					1564:1573	n-3 PUFA deficiency	1555:1573	n-3 PUFA deficiency	1555:1573	Based on a lipopolysaccharide (LPS) model of MIA (LPS injection at embryonic day 17), we showed that n-3 PUFA deficiency 1) alters fatty acid composition of the fetal and adult offspring brain; 2) exacerbates maternal and fetal inflammatory processes with no significant alteration of microglia phenotype, and 3) induces spatial memory deficits in the adult offspring.
29879442	6	45	theme	n-6	1110:1112	arg1	PUFAs					1114:1118	n-6 PUFAs	1110:1118	n-6 PUFAs	1110:1118	PUFAs of the n-3 series ('n-3 PUFAs', also known as omega-3) exhibit anti-inflammatory/pro-resolution properties and promote immune functions, while PUFAs of the n-6 series ('n-6 PUFAs' or omega-6) favor pro-inflammatory responses.
29879442	9	46	theme	memory	1783:1788	arg1	deficits					1790:1797	spatial memory deficits	1775:1797	spatial memory deficits	1775:1797	Based on a lipopolysaccharide (LPS) model of MIA (LPS injection at embryonic day 17), we showed that n-3 PUFA deficiency 1) alters fatty acid composition of the fetal and adult offspring brain; 2) exacerbates maternal and fetal inflammatory processes with no significant alteration of microglia phenotype, and 3) induces spatial memory deficits in the adult offspring.
29879442	3	47	theme	Accumulating	511:522	arg1	evidence					524:531	Accumulating evidence	511:531	Accumulating evidence	511:531	Accumulating evidence point out that proinflammatory cytokines produced both in the maternal and fetal compartments are responsible for social, cognitive and emotional behavioral deficits in the offspring.
29879442	5	48	theme	immune	901:906	arg1	response					925:932	the immune and inflammatory response	897:932	the immune and inflammatory response	897:932	PUFAs and their bioactive derivatives can promote or inhibit many aspects of the immune and inflammatory response.
29879442	9	49	theme	embryonic	1521:1529	arg1	day					1531:1533	embryonic day 17	1521:1536	embryonic day 17	1521:1536	Based on a lipopolysaccharide (LPS) model of MIA (LPS injection at embryonic day 17), we showed that n-3 PUFA deficiency 1) alters fatty acid composition of the fetal and adult offspring brain; 2) exacerbates maternal and fetal inflammatory processes with no significant alteration of microglia phenotype, and 3) induces spatial memory deficits in the adult offspring.
29879442	0	50	theme	memory	72:77	arg1	deficits					79:86	spatial memory deficits	64:86	spatial memory deficits in mice exposed to lipopolysaccharide during gestation	64:141	Dietary omega-3 deficiency exacerbates inflammation and reveals spatial memory deficits in mice exposed to lipopolysaccharide during gestation.
29879442	9	51	dep	exacerbates	1651:1661	arg1	2					1648:1648	2	1648:1648	2	1648:1648	Based on a lipopolysaccharide (LPS) model of MIA (LPS injection at embryonic day 17), we showed that n-3 PUFA deficiency 1) alters fatty acid composition of the fetal and adult offspring brain; 2) exacerbates maternal and fetal inflammatory processes with no significant alteration of microglia phenotype, and 3) induces spatial memory deficits in the adult offspring.
29879442	9	52	theme	adult	1806:1810	arg1	offspring					1812:1820	the adult offspring	1802:1820	the adult offspring	1802:1820	Based on a lipopolysaccharide (LPS) model of MIA (LPS injection at embryonic day 17), we showed that n-3 PUFA deficiency 1) alters fatty acid composition of the fetal and adult offspring brain; 2) exacerbates maternal and fetal inflammatory processes with no significant alteration of microglia phenotype, and 3) induces spatial memory deficits in the adult offspring.
29879442	7	53	theme	MIA	1265:1267	arg1	consequences					1249:1260	the consequences	1245:1260	the consequences of MIA on brain development	1245:1288	The present study aimed at providing insight into the effects of n-3 PUFAs on the consequences of MIA on brain development.
29879442	2	54	theme	causal	355:360	arg1	link					362:365	a causal link	353:365	a causal link between maternal inflammatory activation during pregnancy and behavioural impairment relevant to neurodevelopmental disorders in the offspring	353:508	Animal models of in utero inflammation further revealed a causal link between maternal inflammatory activation during pregnancy and behavioural impairment relevant to neurodevelopmental disorders in the offspring.
29879442	5	55	theme	many	881:884	arg1	aspects					886:892	many aspects	881:892	many aspects of the immune and inflammatory response	881:932	PUFAs and their bioactive derivatives can promote or inhibit many aspects of the immune and inflammatory response.
29879442	9	56	theme	acid	1591:1594	arg1	composition					1596:1606	fatty acid composition	1585:1606	fatty acid composition of the fetal and adult offspring brain	1585:1645	Based on a lipopolysaccharide (LPS) model of MIA (LPS injection at embryonic day 17), we showed that n-3 PUFA deficiency 1) alters fatty acid composition of the fetal and adult offspring brain; 2) exacerbates maternal and fetal inflammatory processes with no significant alteration of microglia phenotype, and 3) induces spatial memory deficits in the adult offspring.
29879442	10	57	theme	negative	1847:1854	arg1	correlation					1856:1866	a strong negative correlation	1838:1866	a strong negative correlation between brain content in n-3 PUFA and cytokine production in MIA-exposed fetuses	1838:1947	We also showed a strong negative correlation between brain content in n-3 PUFA and cytokine production in MIA-exposed fetuses.
29879442	6	58	dep	series	952:957	arg1	PUFAs					965:969	n-3 PUFAs	961:969	'n-3 PUFAs'	960:970	PUFAs of the n-3 series ('n-3 PUFAs', also known as omega-3) exhibit anti-inflammatory/pro-resolution properties and promote immune functions, while PUFAs of the n-6 series ('n-6 PUFAs' or omega-6) favor pro-inflammatory responses.
29879442	2	59	theme	inflammatory	384:395	arg1	activation					397:406	maternal inflammatory activation	375:406	maternal inflammatory activation during pregnancy and behavioural impairment relevant to neurodevelopmental disorders in the offspring	375:508	Animal models of in utero inflammation further revealed a causal link between maternal inflammatory activation during pregnancy and behavioural impairment relevant to neurodevelopmental disorders in the offspring.
29879442	9	60	theme	fetal	1615:1619	arg1	brain					1641:1645	the fetal and adult offspring brain	1611:1645	the fetal and adult offspring brain	1611:1645	Based on a lipopolysaccharide (LPS) model of MIA (LPS injection at embryonic day 17), we showed that n-3 PUFA deficiency 1) alters fatty acid composition of the fetal and adult offspring brain; 2) exacerbates maternal and fetal inflammatory processes with no significant alteration of microglia phenotype, and 3) induces spatial memory deficits in the adult offspring.
29879442	1	61	theme	risk	251:254	arg1	factor					256:261	a risk factor	249:261	a risk factor for neurodevelopmental disorders	249:294	Maternal immune activation (MIA) is a common environmental insult on the developing brain and represents a risk factor for neurodevelopmental disorders.
29879442	9	62	theme	adult	1625:1629	arg1	brain					1641:1645	the fetal and adult offspring brain	1611:1645	the fetal and adult offspring brain	1611:1645	Based on a lipopolysaccharide (LPS) model of MIA (LPS injection at embryonic day 17), we showed that n-3 PUFA deficiency 1) alters fatty acid composition of the fetal and adult offspring brain; 2) exacerbates maternal and fetal inflammatory processes with no significant alteration of microglia phenotype, and 3) induces spatial memory deficits in the adult offspring.
29879442	7	63	theme	brain	1272:1276	arg1	development					1278:1288	brain development	1272:1288	brain development	1272:1288	The present study aimed at providing insight into the effects of n-3 PUFAs on the consequences of MIA on brain development.
29879442	4	64	with	acids	773:777	arg1	activities					808:817	potent immunomodulatory activities	784:817	potent immunomodulatory activities	784:817	Polyunsaturated fatty acids (PUFAs) are essential fatty acids with potent immunomodulatory activities.
29879442	9	65	dep	induces	1767:1773	arg1	3					1764:1764	3	1764:1764	3	1764:1764	Based on a lipopolysaccharide (LPS) model of MIA (LPS injection at embryonic day 17), we showed that n-3 PUFA deficiency 1) alters fatty acid composition of the fetal and adult offspring brain; 2) exacerbates maternal and fetal inflammatory processes with no significant alteration of microglia phenotype, and 3) induces spatial memory deficits in the adult offspring.
29879442	9	66	theme	fatty	1585:1589	arg1	composition					1596:1606	fatty acid composition	1585:1606	fatty acid composition of the fetal and adult offspring brain	1585:1645	Based on a lipopolysaccharide (LPS) model of MIA (LPS injection at embryonic day 17), we showed that n-3 PUFA deficiency 1) alters fatty acid composition of the fetal and adult offspring brain; 2) exacerbates maternal and fetal inflammatory processes with no significant alteration of microglia phenotype, and 3) induces spatial memory deficits in the adult offspring.
29879442	9	67	theme	brain	1641:1645	arg1	composition					1596:1606	fatty acid composition	1585:1606	fatty acid composition of the fetal and adult offspring brain	1585:1645	Based on a lipopolysaccharide (LPS) model of MIA (LPS injection at embryonic day 17), we showed that n-3 PUFA deficiency 1) alters fatty acid composition of the fetal and adult offspring brain; 2) exacerbates maternal and fetal inflammatory processes with no significant alteration of microglia phenotype, and 3) induces spatial memory deficits in the adult offspring.
29879442	11	68	theme	neurodevelopmental	2231:2248	arg1	disorders					2250:2258	neurodevelopmental disorders	2231:2258	neurodevelopmental disorders	2231:2258	Overall, our study is the first to address the deleterious effects of n-3 PUFA deficiency on brain lipid composition, inflammation and memory performances in MIA-exposed animals and indicates that it should be considered as a potent environmental risk factor for the apparition of neurodevelopmental disorders.
29879442	8	69	dep	defects	1418:1424	arg1	both					1349:1352	both	1349:1352	both	1349:1352	We hypothesized that a reduction in n-3 PUFAs exacerbates both maternal and fetal inflammatory responses to MIA and later-life defects in memory in the offspring.
29879442	8	69	dep	defects	1418:1424	arg1	later-life					1407:1416	later-life	1407:1416	later-life	1407:1416	We hypothesized that a reduction in n-3 PUFAs exacerbates both maternal and fetal inflammatory responses to MIA and later-life defects in memory in the offspring.
29879442	11	70	theme	MIA-exposed	2108:2118	arg1	animals					2120:2126	MIA-exposed animals	2108:2126	MIA-exposed animals	2108:2126	Overall, our study is the first to address the deleterious effects of n-3 PUFA deficiency on brain lipid composition, inflammation and memory performances in MIA-exposed animals and indicates that it should be considered as a potent environmental risk factor for the apparition of neurodevelopmental disorders.
29879442	4	71	theme	fatty	733:737	arg1	acids					739:743	Polyunsaturated fatty acids	717:743	Polyunsaturated fatty acids (PUFAs)	717:751	Polyunsaturated fatty acids (PUFAs) are essential fatty acids with potent immunomodulatory activities.
29879442	4	71	theme	fatty	733:737	arg1	acids					773:777	essential fatty acids	757:777	essential fatty acids with potent immunomodulatory activities	757:817	Polyunsaturated fatty acids (PUFAs) are essential fatty acids with potent immunomodulatory activities.
29879442	4	71	theme	fatty	733:737	arg1	PUFAs					746:750	PUFAs	746:750	PUFAs	746:750	Polyunsaturated fatty acids (PUFAs) are essential fatty acids with potent immunomodulatory activities.
29879442	10	72	theme	n-3	1893:1895	arg1	PUFA					1897:1900	n-3 PUFA	1893:1900	n-3 PUFA	1893:1900	We also showed a strong negative correlation between brain content in n-3 PUFA and cytokine production in MIA-exposed fetuses.
29879442	2	73	theme	inflammation	323:334	arg1	models					304:309	Animal models	297:309	Animal models of in utero inflammation	297:334	Animal models of in utero inflammation further revealed a causal link between maternal inflammatory activation during pregnancy and behavioural impairment relevant to neurodevelopmental disorders in the offspring.
29879442	1	74	theme	common	182:187	arg1	activation					160:169	Maternal immune activation	144:169	Maternal immune activation (MIA)	144:175	Maternal immune activation (MIA) is a common environmental insult on the developing brain and represents a risk factor for neurodevelopmental disorders.
29879442	1	74	theme	common	182:187	arg1	insult					203:208	a common environmental insult	180:208	a common environmental insult on the developing brain	180:232	Maternal immune activation (MIA) is a common environmental insult on the developing brain and represents a risk factor for neurodevelopmental disorders.
29879442	9	75	dep	alters	1578:1583	arg1	1					1575:1575	1	1575:1575	1	1575:1575	Based on a lipopolysaccharide (LPS) model of MIA (LPS injection at embryonic day 17), we showed that n-3 PUFA deficiency 1) alters fatty acid composition of the fetal and adult offspring brain; 2) exacerbates maternal and fetal inflammatory processes with no significant alteration of microglia phenotype, and 3) induces spatial memory deficits in the adult offspring.
29879442	10	76	theme	MIA-exposed	1929:1939	arg1	fetuses					1941:1947	MIA-exposed fetuses	1929:1947	MIA-exposed fetuses	1929:1947	We also showed a strong negative correlation between brain content in n-3 PUFA and cytokine production in MIA-exposed fetuses.
29879442	2	77	theme	in	314:315	arg1	inflammation					323:334	in utero inflammation	314:334	in utero inflammation	314:334	Animal models of in utero inflammation further revealed a causal link between maternal inflammatory activation during pregnancy and behavioural impairment relevant to neurodevelopmental disorders in the offspring.
29879442	10	78	from	content	1882:1888	arg1	PUFA					1897:1900	n-3 PUFA	1893:1900	n-3 PUFA	1893:1900	We also showed a strong negative correlation between brain content in n-3 PUFA and cytokine production in MIA-exposed fetuses.
29879442	10	78	from	content	1882:1888	arg1	production					1915:1924	cytokine production	1906:1924	cytokine production	1906:1924	We also showed a strong negative correlation between brain content in n-3 PUFA and cytokine production in MIA-exposed fetuses.
29879442	6	79	theme	anti-inflammatory/pro-resolution	1004:1035	arg1	properties					1037:1046	anti-inflammatory/pro-resolution properties	1004:1046	anti-inflammatory/pro-resolution properties	1004:1046	PUFAs of the n-3 series ('n-3 PUFAs', also known as omega-3) exhibit anti-inflammatory/pro-resolution properties and promote immune functions, while PUFAs of the n-6 series ('n-6 PUFAs' or omega-6) favor pro-inflammatory responses.
29879442	11	80	theme	PUFA	2024:2027	arg1	deficiency					2029:2038	n-3 PUFA deficiency	2020:2038	n-3 PUFA deficiency	2020:2038	Overall, our study is the first to address the deleterious effects of n-3 PUFA deficiency on brain lipid composition, inflammation and memory performances in MIA-exposed animals and indicates that it should be considered as a potent environmental risk factor for the apparition of neurodevelopmental disorders.
29879442	4	81	theme	essential	757:765	arg1	acids					739:743	Polyunsaturated fatty acids	717:743	Polyunsaturated fatty acids (PUFAs)	717:751	Polyunsaturated fatty acids (PUFAs) are essential fatty acids with potent immunomodulatory activities.
29879442	4	81	theme	essential	757:765	arg1	acids					773:777	essential fatty acids	757:777	essential fatty acids with potent immunomodulatory activities	757:817	Polyunsaturated fatty acids (PUFAs) are essential fatty acids with potent immunomodulatory activities.
29879442	3	82	theme	cognitive	655:663	arg1	deficits					690:697	social, cognitive and emotional behavioral deficits	647:697	social, cognitive and emotional behavioral deficits in the offspring	647:714	Accumulating evidence point out that proinflammatory cytokines produced both in the maternal and fetal compartments are responsible for social, cognitive and emotional behavioral deficits in the offspring.
29879442	5	83	theme	response	925:932	arg1	aspects					886:892	many aspects	881:892	many aspects of the immune and inflammatory response	881:932	PUFAs and their bioactive derivatives can promote or inhibit many aspects of the immune and inflammatory response.
29879442	9	84	theme	inflammatory	1682:1693	arg1	processes					1695:1703	maternal and fetal inflammatory processes	1663:1703	maternal and fetal inflammatory processes	1663:1703	Based on a lipopolysaccharide (LPS) model of MIA (LPS injection at embryonic day 17), we showed that n-3 PUFA deficiency 1) alters fatty acid composition of the fetal and adult offspring brain; 2) exacerbates maternal and fetal inflammatory processes with no significant alteration of microglia phenotype, and 3) induces spatial memory deficits in the adult offspring.
29879442	0	85	from	deficits	79:86	arg1	mice					91:94	mice	91:94	mice exposed to lipopolysaccharide during gestation	91:141	Dietary omega-3 deficiency exacerbates inflammation and reveals spatial memory deficits in mice exposed to lipopolysaccharide during gestation.
29879442	11	86	from	performances	2092:2103	arg1	animals					2120:2126	MIA-exposed animals	2108:2126	MIA-exposed animals	2108:2126	Overall, our study is the first to address the deleterious effects of n-3 PUFA deficiency on brain lipid composition, inflammation and memory performances in MIA-exposed animals and indicates that it should be considered as a potent environmental risk factor for the apparition of neurodevelopmental disorders.
29879442	3	87	theme	social	647:652	arg1	deficits					690:697	social, cognitive and emotional behavioral deficits	647:697	social, cognitive and emotional behavioral deficits in the offspring	647:714	Accumulating evidence point out that proinflammatory cytokines produced both in the maternal and fetal compartments are responsible for social, cognitive and emotional behavioral deficits in the offspring.
29879442	11	88	dep	first	1976:1980	arg1	indicates					2132:2140	indicates	2132:2140	indicates that it should be considered as a potent environmental risk factor for the apparition of neurodevelopmental disorders	2132:2258	Overall, our study is the first to address the deleterious effects of n-3 PUFA deficiency on brain lipid composition, inflammation and memory performances in MIA-exposed animals and indicates that it should be considered as a potent environmental risk factor for the apparition of neurodevelopmental disorders.
29879442	11	88	dep	first	1976:1980	arg1	address					1985:1991	address	1985:1991	to address the deleterious effects of n-3 PUFA deficiency on brain lipid composition, inflammation and memory performances in MIA-exposed animals	1982:2126	Overall, our study is the first to address the deleterious effects of n-3 PUFA deficiency on brain lipid composition, inflammation and memory performances in MIA-exposed animals and indicates that it should be considered as a potent environmental risk factor for the apparition of neurodevelopmental disorders.
29879442	11	89	theme	lipid	2049:2053	arg1	composition					2055:2065	brain lipid composition	2043:2065	brain lipid composition	2043:2065	Overall, our study is the first to address the deleterious effects of n-3 PUFA deficiency on brain lipid composition, inflammation and memory performances in MIA-exposed animals and indicates that it should be considered as a potent environmental risk factor for the apparition of neurodevelopmental disorders.
29879442	1	90	theme	Maternal	144:151	arg1	MIA					172:174	MIA	172:174	MIA	172:174	Maternal immune activation (MIA) is a common environmental insult on the developing brain and represents a risk factor for neurodevelopmental disorders.
29879442	1	90	theme	Maternal	144:151	arg1	insult					203:208	a common environmental insult	180:208	a common environmental insult on the developing brain	180:232	Maternal immune activation (MIA) is a common environmental insult on the developing brain and represents a risk factor for neurodevelopmental disorders.
29879442	1	90	theme	Maternal	144:151	arg1	activation					160:169	Maternal immune activation	144:169	Maternal immune activation (MIA)	144:175	Maternal immune activation (MIA) is a common environmental insult on the developing brain and represents a risk factor for neurodevelopmental disorders.
29879442	9	91	theme	significant	1713:1723	arg1	alteration					1725:1734	no significant alteration	1710:1734	no significant alteration of microglia phenotype	1710:1757	Based on a lipopolysaccharide (LPS) model of MIA (LPS injection at embryonic day 17), we showed that n-3 PUFA deficiency 1) alters fatty acid composition of the fetal and adult offspring brain; 2) exacerbates maternal and fetal inflammatory processes with no significant alteration of microglia phenotype, and 3) induces spatial memory deficits in the adult offspring.
29879442	8	92	theme	inflammatory	1373:1384	arg1	responses					1386:1394	maternal and fetal inflammatory responses	1354:1394	maternal and fetal inflammatory responses to MIA	1354:1401	We hypothesized that a reduction in n-3 PUFAs exacerbates both maternal and fetal inflammatory responses to MIA and later-life defects in memory in the offspring.
29879442	4	93	theme	potent	784:789	arg1	activities					808:817	potent immunomodulatory activities	784:817	potent immunomodulatory activities	784:817	Polyunsaturated fatty acids (PUFAs) are essential fatty acids with potent immunomodulatory activities.
29879442	11	94	theme	n-3	2020:2022	arg1	PUFA					2024:2027	n-3 PUFA	2020:2027	n-3 PUFA deficiency	2020:2038	Overall, our study is the first to address the deleterious effects of n-3 PUFA deficiency on brain lipid composition, inflammation and memory performances in MIA-exposed animals and indicates that it should be considered as a potent environmental risk factor for the apparition of neurodevelopmental disorders.
29879442	3	95	theme	emotional	669:677	arg1	deficits					690:697	social, cognitive and emotional behavioral deficits	647:697	social, cognitive and emotional behavioral deficits in the offspring	647:714	Accumulating evidence point out that proinflammatory cytokines produced both in the maternal and fetal compartments are responsible for social, cognitive and emotional behavioral deficits in the offspring.
29879442	3	96	dep	compartments	614:625	arg1	both					583:586	both	583:586	both	583:586	Accumulating evidence point out that proinflammatory cytokines produced both in the maternal and fetal compartments are responsible for social, cognitive and emotional behavioral deficits in the offspring.
29879442	11	97	theme	environmental	2183:2195	arg1	factor					2202:2207	a potent environmental risk factor	2174:2207	a potent environmental risk factor for the apparition of neurodevelopmental disorders	2174:2258	Overall, our study is the first to address the deleterious effects of n-3 PUFA deficiency on brain lipid composition, inflammation and memory performances in MIA-exposed animals and indicates that it should be considered as a potent environmental risk factor for the apparition of neurodevelopmental disorders.
29879442	11	97	theme	environmental	2183:2195	arg1	it					2147:2148	it	2147:2148	it	2147:2148	Overall, our study is the first to address the deleterious effects of n-3 PUFA deficiency on brain lipid composition, inflammation and memory performances in MIA-exposed animals and indicates that it should be considered as a potent environmental risk factor for the apparition of neurodevelopmental disorders.
29879442	2	98	from	disorders	483:491	arg1	offspring					500:508	the offspring	496:508	the offspring	496:508	Animal models of in utero inflammation further revealed a causal link between maternal inflammatory activation during pregnancy and behavioural impairment relevant to neurodevelopmental disorders in the offspring.
29879442	9	99	theme	phenotype	1749:1757	arg1	alteration					1725:1734	no significant alteration	1710:1734	no significant alteration of microglia phenotype	1710:1757	Based on a lipopolysaccharide (LPS) model of MIA (LPS injection at embryonic day 17), we showed that n-3 PUFA deficiency 1) alters fatty acid composition of the fetal and adult offspring brain; 2) exacerbates maternal and fetal inflammatory processes with no significant alteration of microglia phenotype, and 3) induces spatial memory deficits in the adult offspring.
29879442	8	100	theme	maternal	1354:1361	arg1	responses					1386:1394	maternal and fetal inflammatory responses	1354:1394	maternal and fetal inflammatory responses to MIA	1354:1401	We hypothesized that a reduction in n-3 PUFAs exacerbates both maternal and fetal inflammatory responses to MIA and later-life defects in memory in the offspring.
29879442	7	101	theme	PUFAs	1236:1240	arg1	effects					1221:1227	the effects	1217:1227	the effects of n-3 PUFAs on the consequences of MIA on brain development	1217:1288	The present study aimed at providing insight into the effects of n-3 PUFAs on the consequences of MIA on brain development.
29879442	3	102	dep	point	533:537	arg1	out					539:541	out	539:541	out	539:541	Accumulating evidence point out that proinflammatory cytokines produced both in the maternal and fetal compartments are responsible for social, cognitive and emotional behavioral deficits in the offspring.
29879442	6	103	theme	immune	1060:1065	arg1	functions					1067:1075	immune functions	1060:1075	immune functions	1060:1075	PUFAs of the n-3 series ('n-3 PUFAs', also known as omega-3) exhibit anti-inflammatory/pro-resolution properties and promote immune functions, while PUFAs of the n-6 series ('n-6 PUFAs' or omega-6) favor pro-inflammatory responses.
29879442	8	104	theme	fetal	1367:1371	arg1	responses					1386:1394	maternal and fetal inflammatory responses	1354:1394	maternal and fetal inflammatory responses to MIA	1354:1401	We hypothesized that a reduction in n-3 PUFAs exacerbates both maternal and fetal inflammatory responses to MIA and later-life defects in memory in the offspring.
29879442	8	105	from	reduction	1314:1322	arg1	PUFAs					1331:1335	n-3 PUFAs	1327:1335	n-3 PUFAs	1327:1335	We hypothesized that a reduction in n-3 PUFAs exacerbates both maternal and fetal inflammatory responses to MIA and later-life defects in memory in the offspring.
29879442	3	106	from	deficits	690:697	arg1	offspring					706:714	the offspring	702:714	the offspring	702:714	Accumulating evidence point out that proinflammatory cytokines produced both in the maternal and fetal compartments are responsible for social, cognitive and emotional behavioral deficits in the offspring.
29879442	0	107	theme	omega-3	8:14	arg1	deficiency					16:25	Dietary omega-3 deficiency	0:25	Dietary omega-3 deficiency	0:25	Dietary omega-3 deficiency exacerbates inflammation and reveals spatial memory deficits in mice exposed to lipopolysaccharide during gestation.
29879442	9	108	theme	n-3	1555:1557	arg1	PUFA					1559:1562	n-3 PUFA	1555:1562	n-3 PUFA deficiency	1555:1573	Based on a lipopolysaccharide (LPS) model of MIA (LPS injection at embryonic day 17), we showed that n-3 PUFA deficiency 1) alters fatty acid composition of the fetal and adult offspring brain; 2) exacerbates maternal and fetal inflammatory processes with no significant alteration of microglia phenotype, and 3) induces spatial memory deficits in the adult offspring.
29879442	9	109	theme	spatial	1775:1781	arg1	deficits					1790:1797	spatial memory deficits	1775:1797	spatial memory deficits	1775:1797	Based on a lipopolysaccharide (LPS) model of MIA (LPS injection at embryonic day 17), we showed that n-3 PUFA deficiency 1) alters fatty acid composition of the fetal and adult offspring brain; 2) exacerbates maternal and fetal inflammatory processes with no significant alteration of microglia phenotype, and 3) induces spatial memory deficits in the adult offspring.
29879442	2	110	theme	neurodevelopmental	464:481	arg1	disorders					483:491	neurodevelopmental disorders	464:491	neurodevelopmental disorders in the offspring	464:508	Animal models of in utero inflammation further revealed a causal link between maternal inflammatory activation during pregnancy and behavioural impairment relevant to neurodevelopmental disorders in the offspring.
29879442	2	111	theme	relevant	452:459	arg1	pregnancy					415:423	pregnancy	415:423	pregnancy	415:423	Animal models of in utero inflammation further revealed a causal link between maternal inflammatory activation during pregnancy and behavioural impairment relevant to neurodevelopmental disorders in the offspring.
29879442	6	112	theme	series	1101:1106	arg1	PUFAs					1084:1088	PUFAs	1084:1088	PUFAs of the n-6 series ('n-6 PUFAs' or omega-6)	1084:1131	PUFAs of the n-3 series ('n-3 PUFAs', also known as omega-3) exhibit anti-inflammatory/pro-resolution properties and promote immune functions, while PUFAs of the n-6 series ('n-6 PUFAs' or omega-6) favor pro-inflammatory responses.
29879442	2	113	theme	behavioural	429:439	arg1	impairment					441:450	behavioural impairment	429:450	behavioural impairment	429:450	Animal models of in utero inflammation further revealed a causal link between maternal inflammatory activation during pregnancy and behavioural impairment relevant to neurodevelopmental disorders in the offspring.
29879442	0	114	theme	spatial	64:70	arg1	deficits					79:86	spatial memory deficits	64:86	spatial memory deficits in mice exposed to lipopolysaccharide during gestation	64:141	Dietary omega-3 deficiency exacerbates inflammation and reveals spatial memory deficits in mice exposed to lipopolysaccharide during gestation.
29879442	8	115	dep	both	1349:1352	arg1	responses					1386:1394	maternal and fetal inflammatory responses	1354:1394	maternal and fetal inflammatory responses to MIA	1354:1401	We hypothesized that a reduction in n-3 PUFAs exacerbates both maternal and fetal inflammatory responses to MIA and later-life defects in memory in the offspring.
29879442	3	116	theme	proinflammatory	548:562	arg1	cytokines					564:572	proinflammatory cytokines	548:572	proinflammatory cytokines produced both in the maternal and fetal compartments	548:625	Accumulating evidence point out that proinflammatory cytokines produced both in the maternal and fetal compartments are responsible for social, cognitive and emotional behavioral deficits in the offspring.
29879442	10	117	theme	strong	1840:1845	arg1	correlation					1856:1866	a strong negative correlation	1838:1866	a strong negative correlation between brain content in n-3 PUFA and cytokine production in MIA-exposed fetuses	1838:1947	We also showed a strong negative correlation between brain content in n-3 PUFA and cytokine production in MIA-exposed fetuses.
29879442	9	118	dep	MIA	1499:1501	arg1	injection					1508:1516	LPS injection	1504:1516	LPS injection at embryonic day 17	1504:1536	Based on a lipopolysaccharide (LPS) model of MIA (LPS injection at embryonic day 17), we showed that n-3 PUFA deficiency 1) alters fatty acid composition of the fetal and adult offspring brain; 2) exacerbates maternal and fetal inflammatory processes with no significant alteration of microglia phenotype, and 3) induces spatial memory deficits in the adult offspring.
29879442	9	119	theme	MIA	1499:1501	arg1	model					1490:1494	a lipopolysaccharide (LPS) model	1463:1494	a lipopolysaccharide (LPS) model of MIA (LPS injection at embryonic day 17)	1463:1537	Based on a lipopolysaccharide (LPS) model of MIA (LPS injection at embryonic day 17), we showed that n-3 PUFA deficiency 1) alters fatty acid composition of the fetal and adult offspring brain; 2) exacerbates maternal and fetal inflammatory processes with no significant alteration of microglia phenotype, and 3) induces spatial memory deficits in the adult offspring.
29879442	6	120	theme	pro-inflammatory	1139:1154	arg1	responses					1156:1164	pro-inflammatory responses	1139:1164	pro-inflammatory responses	1139:1164	PUFAs of the n-3 series ('n-3 PUFAs', also known as omega-3) exhibit anti-inflammatory/pro-resolution properties and promote immune functions, while PUFAs of the n-6 series ('n-6 PUFAs' or omega-6) favor pro-inflammatory responses.
29879442	2	121	theme	maternal	375:382	arg1	activation					397:406	maternal inflammatory activation	375:406	maternal inflammatory activation during pregnancy and behavioural impairment relevant to neurodevelopmental disorders in the offspring	375:508	Animal models of in utero inflammation further revealed a causal link between maternal inflammatory activation during pregnancy and behavioural impairment relevant to neurodevelopmental disorders in the offspring.
29879442	8	122	from	defects	1418:1424	arg1	memory					1429:1434	memory	1429:1434	memory	1429:1434	We hypothesized that a reduction in n-3 PUFAs exacerbates both maternal and fetal inflammatory responses to MIA and later-life defects in memory in the offspring.
30628723	0	0	from	analysis	26:33	arg1	pufferfish					82:91	the blunthead pufferfish	68:91	the blunthead pufferfish	68:91	Comparative histochemical analysis of intestinal glycoconjugates in the blunthead pufferfish Sphoeroides pachygaster and grey triggerfish Balistes capriscus (Teleostei: Tetraodontiformes).
30628723	1	1	theme	grey	296:299	arg1	capriscus					322:330	the grey triggerfish Balistes capriscus	292:330	the grey triggerfish Balistes capriscus from the north-western Ionian Sea	292:364	The localization of intestinal glycoconjugates of the blunthead pufferfish Sphoeroides pachygaster and the grey triggerfish Balistes capriscus from the north-western Ionian Sea was analysed by histochemical methods (PAS, AB pH 2.5, HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA) to assess how evolutionary loss of a functional stomach in S. pachygaster affects intestinal secretions relative to the B. capriscus, which retains the plesiomorphic gastric condition.
30628723	1	2	theme	WGA	458:460	arg1	LTA					526:528	HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA	421:528	LTA	526:528	The localization of intestinal glycoconjugates of the blunthead pufferfish Sphoeroides pachygaster and the grey triggerfish Balistes capriscus from the north-western Ionian Sea was analysed by histochemical methods (PAS, AB pH 2.5, HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA) to assess how evolutionary loss of a functional stomach in S. pachygaster affects intestinal secretions relative to the B. capriscus, which retains the plesiomorphic gastric condition.
30628723	1	2	theme	WGA	458:460	arg1	PAS					405:407	PAS	405:407	PAS	405:407	The localization of intestinal glycoconjugates of the blunthead pufferfish Sphoeroides pachygaster and the grey triggerfish Balistes capriscus from the north-western Ionian Sea was analysed by histochemical methods (PAS, AB pH 2.5, HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA) to assess how evolutionary loss of a functional stomach in S. pachygaster affects intestinal secretions relative to the B. capriscus, which retains the plesiomorphic gastric condition.
30628723	0	3	dep	capriscus	147:155	arg1	Teleostei					158:166	Teleostei	158:166	Teleostei: Tetraodontiformes	158:185	Comparative histochemical analysis of intestinal glycoconjugates in the blunthead pufferfish Sphoeroides pachygaster and grey triggerfish Balistes capriscus (Teleostei: Tetraodontiformes).
30628723	3	4	theme	GlcNAc	843:848	arg1	residuals					850:858	GalNAc and GlcNAc residuals	832:858	GalNAc and GlcNAc residuals	832:858	GalNAc and GlcNAc residuals were present in both, but GalNAc residuals in S. pachygaster were subterminal to sialic acid.
30628723	1	5	theme	triggerfish	301:311	arg1	capriscus					322:330	the grey triggerfish Balistes capriscus	292:330	the grey triggerfish Balistes capriscus from the north-western Ionian Sea	292:364	The localization of intestinal glycoconjugates of the blunthead pufferfish Sphoeroides pachygaster and the grey triggerfish Balistes capriscus from the north-western Ionian Sea was analysed by histochemical methods (PAS, AB pH 2.5, HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA) to assess how evolutionary loss of a functional stomach in S. pachygaster affects intestinal secretions relative to the B. capriscus, which retains the plesiomorphic gastric condition.
30628723	0	6	theme	grey	121:124	arg1	capriscus					147:155	grey triggerfish Balistes capriscus	121:155	grey triggerfish Balistes capriscus (Teleostei: Tetraodontiformes)	121:186	Comparative histochemical analysis of intestinal glycoconjugates in the blunthead pufferfish Sphoeroides pachygaster and grey triggerfish Balistes capriscus (Teleostei: Tetraodontiformes).
30628723	4	7	from	pachygaster	1138:1148	arg1	glycocalyx					1031:1040	a glycocalyx	1029:1040	a glycocalyx that differed in composition between the small intestine and the rectum and was missing from S. pachygaster	1029:1148	Balistes capriscus lacked galactosylated residuals and its enterocytes had a glycocalyx that differed in composition between the small intestine and the rectum and was missing from S. pachygaster.
30628723	4	7	from	pachygaster	1138:1148	arg1	missing					1122:1128	missing	1122:1128	missing	1122:1128	Balistes capriscus lacked galactosylated residuals and its enterocytes had a glycocalyx that differed in composition between the small intestine and the rectum and was missing from S. pachygaster.
30628723	1	8	from	Sea	362:364	arg1	localization					193:204	The localization	189:204	The localization of intestinal glycoconjugates of the blunthead pufferfish Sphoeroides pachygaster and the grey triggerfish Balistes capriscus from the north-western Ionian Sea	189:364	The localization of intestinal glycoconjugates of the blunthead pufferfish Sphoeroides pachygaster and the grey triggerfish Balistes capriscus from the north-western Ionian Sea was analysed by histochemical methods (PAS, AB pH 2.5, HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA) to assess how evolutionary loss of a functional stomach in S. pachygaster affects intestinal secretions relative to the B. capriscus, which retains the plesiomorphic gastric condition.
30628723	1	8	from	Sea	362:364	arg1	pachygaster					276:286	the blunthead pufferfish Sphoeroides pachygaster	239:286	the blunthead pufferfish Sphoeroides pachygaster	239:286	The localization of intestinal glycoconjugates of the blunthead pufferfish Sphoeroides pachygaster and the grey triggerfish Balistes capriscus from the north-western Ionian Sea was analysed by histochemical methods (PAS, AB pH 2.5, HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA) to assess how evolutionary loss of a functional stomach in S. pachygaster affects intestinal secretions relative to the B. capriscus, which retains the plesiomorphic gastric condition.
30628723	1	8	from	Sea	362:364	arg1	capriscus					322:330	the grey triggerfish Balistes capriscus	292:330	the grey triggerfish Balistes capriscus from the north-western Ionian Sea	292:364	The localization of intestinal glycoconjugates of the blunthead pufferfish Sphoeroides pachygaster and the grey triggerfish Balistes capriscus from the north-western Ionian Sea was analysed by histochemical methods (PAS, AB pH 2.5, HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA) to assess how evolutionary loss of a functional stomach in S. pachygaster affects intestinal secretions relative to the B. capriscus, which retains the plesiomorphic gastric condition.
30628723	2	9	theme	sialylation	792:802	arg1	patterns					804:811	more complex sialylation patterns	779:811	more complex sialylation patterns	779:811	Sphoeroides pachygaster had a lower content of acid mucins but more complex sialylation patterns than B. capriscus.
30628723	1	10	theme	Balistes	313:320	arg1	capriscus					322:330	the grey triggerfish Balistes capriscus	292:330	the grey triggerfish Balistes capriscus from the north-western Ionian Sea	292:364	The localization of intestinal glycoconjugates of the blunthead pufferfish Sphoeroides pachygaster and the grey triggerfish Balistes capriscus from the north-western Ionian Sea was analysed by histochemical methods (PAS, AB pH 2.5, HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA) to assess how evolutionary loss of a functional stomach in S. pachygaster affects intestinal secretions relative to the B. capriscus, which retains the plesiomorphic gastric condition.
30628723	1	11	theme	gastric	697:703	arg1	condition					705:713	the plesiomorphic gastric condition	679:713	the plesiomorphic gastric condition	679:713	The localization of intestinal glycoconjugates of the blunthead pufferfish Sphoeroides pachygaster and the grey triggerfish Balistes capriscus from the north-western Ionian Sea was analysed by histochemical methods (PAS, AB pH 2.5, HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA) to assess how evolutionary loss of a functional stomach in S. pachygaster affects intestinal secretions relative to the B. capriscus, which retains the plesiomorphic gastric condition.
30628723	1	12	dep	analysed	370:377	arg1	LTA					526:528	HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA	421:528	LTA	526:528	The localization of intestinal glycoconjugates of the blunthead pufferfish Sphoeroides pachygaster and the grey triggerfish Balistes capriscus from the north-western Ionian Sea was analysed by histochemical methods (PAS, AB pH 2.5, HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA) to assess how evolutionary loss of a functional stomach in S. pachygaster affects intestinal secretions relative to the B. capriscus, which retains the plesiomorphic gastric condition.
30628723	1	12	dep	analysed	370:377	arg1	PAS					405:407	PAS	405:407	PAS	405:407	The localization of intestinal glycoconjugates of the blunthead pufferfish Sphoeroides pachygaster and the grey triggerfish Balistes capriscus from the north-western Ionian Sea was analysed by histochemical methods (PAS, AB pH 2.5, HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA) to assess how evolutionary loss of a functional stomach in S. pachygaster affects intestinal secretions relative to the B. capriscus, which retains the plesiomorphic gastric condition.
30628723	1	12	dep	analysed	370:377	arg1	pH					413:414	AB pH 2.5	410:418	AB pH 2.5	410:418	The localization of intestinal glycoconjugates of the blunthead pufferfish Sphoeroides pachygaster and the grey triggerfish Balistes capriscus from the north-western Ionian Sea was analysed by histochemical methods (PAS, AB pH 2.5, HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA) to assess how evolutionary loss of a functional stomach in S. pachygaster affects intestinal secretions relative to the B. capriscus, which retains the plesiomorphic gastric condition.
30628723	1	12	dep	analysed	370:377	arg1	HID					421:423	HID	421:423	HID	421:423	The localization of intestinal glycoconjugates of the blunthead pufferfish Sphoeroides pachygaster and the grey triggerfish Balistes capriscus from the north-western Ionian Sea was analysed by histochemical methods (PAS, AB pH 2.5, HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA) to assess how evolutionary loss of a functional stomach in S. pachygaster affects intestinal secretions relative to the B. capriscus, which retains the plesiomorphic gastric condition.
30628723	1	13	from	pachygaster	593:603	arg1	loss					558:561	evolutionary loss	545:561	evolutionary loss of a functional stomach in S. pachygaster	545:603	The localization of intestinal glycoconjugates of the blunthead pufferfish Sphoeroides pachygaster and the grey triggerfish Balistes capriscus from the north-western Ionian Sea was analysed by histochemical methods (PAS, AB pH 2.5, HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA) to assess how evolutionary loss of a functional stomach in S. pachygaster affects intestinal secretions relative to the B. capriscus, which retains the plesiomorphic gastric condition.
30628723	1	14	theme	capriscus	322:330	arg1	localization					193:204	The localization	189:204	The localization of intestinal glycoconjugates of the blunthead pufferfish Sphoeroides pachygaster and the grey triggerfish Balistes capriscus from the north-western Ionian Sea	189:364	The localization of intestinal glycoconjugates of the blunthead pufferfish Sphoeroides pachygaster and the grey triggerfish Balistes capriscus from the north-western Ionian Sea was analysed by histochemical methods (PAS, AB pH 2.5, HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA) to assess how evolutionary loss of a functional stomach in S. pachygaster affects intestinal secretions relative to the B. capriscus, which retains the plesiomorphic gastric condition.
30628723	0	15	theme	Balistes	138:145	arg1	capriscus					147:155	grey triggerfish Balistes capriscus	121:155	grey triggerfish Balistes capriscus (Teleostei: Tetraodontiformes)	121:186	Comparative histochemical analysis of intestinal glycoconjugates in the blunthead pufferfish Sphoeroides pachygaster and grey triggerfish Balistes capriscus (Teleostei: Tetraodontiformes).
30628723	1	16	theme	AB	410:411	arg1	PAS					405:407	PAS	405:407	PAS	405:407	The localization of intestinal glycoconjugates of the blunthead pufferfish Sphoeroides pachygaster and the grey triggerfish Balistes capriscus from the north-western Ionian Sea was analysed by histochemical methods (PAS, AB pH 2.5, HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA) to assess how evolutionary loss of a functional stomach in S. pachygaster affects intestinal secretions relative to the B. capriscus, which retains the plesiomorphic gastric condition.
30628723	1	16	theme	AB	410:411	arg1	pH					413:414	AB pH 2.5	410:418	AB pH 2.5	410:418	The localization of intestinal glycoconjugates of the blunthead pufferfish Sphoeroides pachygaster and the grey triggerfish Balistes capriscus from the north-western Ionian Sea was analysed by histochemical methods (PAS, AB pH 2.5, HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA) to assess how evolutionary loss of a functional stomach in S. pachygaster affects intestinal secretions relative to the B. capriscus, which retains the plesiomorphic gastric condition.
30628723	2	17	theme	mucins	768:773	arg1	content					752:758	a lower content	744:758	a lower content of acid mucins	744:773	Sphoeroides pachygaster had a lower content of acid mucins but more complex sialylation patterns than B. capriscus.
30628723	1	18	from	stomach	579:585	arg1	pachygaster					593:603	S. pachygaster	590:603	S. pachygaster	590:603	The localization of intestinal glycoconjugates of the blunthead pufferfish Sphoeroides pachygaster and the grey triggerfish Balistes capriscus from the north-western Ionian Sea was analysed by histochemical methods (PAS, AB pH 2.5, HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA) to assess how evolutionary loss of a functional stomach in S. pachygaster affects intestinal secretions relative to the B. capriscus, which retains the plesiomorphic gastric condition.
30628723	0	19	theme	triggerfish	126:136	arg1	capriscus					147:155	grey triggerfish Balistes capriscus	121:155	grey triggerfish Balistes capriscus (Teleostei: Tetraodontiformes)	121:186	Comparative histochemical analysis of intestinal glycoconjugates in the blunthead pufferfish Sphoeroides pachygaster and grey triggerfish Balistes capriscus (Teleostei: Tetraodontiformes).
30628723	1	20	theme	intestinal	613:622	arg1	secretions					624:633	intestinal secretions	613:633	intestinal secretions relative to the B. capriscus	613:662	The localization of intestinal glycoconjugates of the blunthead pufferfish Sphoeroides pachygaster and the grey triggerfish Balistes capriscus from the north-western Ionian Sea was analysed by histochemical methods (PAS, AB pH 2.5, HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA) to assess how evolutionary loss of a functional stomach in S. pachygaster affects intestinal secretions relative to the B. capriscus, which retains the plesiomorphic gastric condition.
30628723	0	21	theme	histochemical	12:24	arg1	analysis					26:33	Comparative histochemical analysis	0:33	Comparative histochemical analysis of intestinal glycoconjugates in the blunthead pufferfish	0:91	Comparative histochemical analysis of intestinal glycoconjugates in the blunthead pufferfish Sphoeroides pachygaster and grey triggerfish Balistes capriscus (Teleostei: Tetraodontiformes).
30628723	5	22	theme	ecological	1166:1175	arg1	implications					1177:1188	Functional and ecological implications	1151:1188	Functional and ecological implications of these findings	1151:1206	Functional and ecological implications of these findings are discussed.
30628723	4	23	contain	had	1025:1027	arg2	missing					1122:1128	missing	1122:1128	missing	1122:1128	Balistes capriscus lacked galactosylated residuals and its enterocytes had a glycocalyx that differed in composition between the small intestine and the rectum and was missing from S. pachygaster.
30628723	4	23	contain	had	1025:1027	arg1	enterocytes					1013:1023	its enterocytes	1009:1023	its enterocytes	1009:1023	Balistes capriscus lacked galactosylated residuals and its enterocytes had a glycocalyx that differed in composition between the small intestine and the rectum and was missing from S. pachygaster.
30628723	4	23	contain	had	1025:1027	arg2	glycocalyx					1031:1040	a glycocalyx	1029:1040	a glycocalyx that differed in composition between the small intestine and the rectum and was missing from S. pachygaster	1029:1148	Balistes capriscus lacked galactosylated residuals and its enterocytes had a glycocalyx that differed in composition between the small intestine and the rectum and was missing from S. pachygaster.
30628723	2	24	theme	Sphoeroides	716:726	arg1	pachygaster					728:738	Sphoeroides pachygaster	716:738	Sphoeroides pachygaster	716:738	Sphoeroides pachygaster had a lower content of acid mucins but more complex sialylation patterns than B. capriscus.
30628723	1	25	theme	intestinal	209:218	arg1	glycoconjugates					220:234	intestinal glycoconjugates	209:234	intestinal glycoconjugates	209:234	The localization of intestinal glycoconjugates of the blunthead pufferfish Sphoeroides pachygaster and the grey triggerfish Balistes capriscus from the north-western Ionian Sea was analysed by histochemical methods (PAS, AB pH 2.5, HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA) to assess how evolutionary loss of a functional stomach in S. pachygaster affects intestinal secretions relative to the B. capriscus, which retains the plesiomorphic gastric condition.
30628723	0	26	theme	Comparative	0:10	arg1	analysis					26:33	Comparative histochemical analysis	0:33	Comparative histochemical analysis of intestinal glycoconjugates in the blunthead pufferfish	0:91	Comparative histochemical analysis of intestinal glycoconjugates in the blunthead pufferfish Sphoeroides pachygaster and grey triggerfish Balistes capriscus (Teleostei: Tetraodontiformes).
30628723	5	27	theme	Functional	1151:1160	arg1	implications					1177:1188	Functional and ecological implications	1151:1188	Functional and ecological implications of these findings	1151:1206	Functional and ecological implications of these findings are discussed.
30628723	2	28	theme	acid	763:766	arg1	mucins					768:773	acid mucins	763:773	acid mucins	763:773	Sphoeroides pachygaster had a lower content of acid mucins but more complex sialylation patterns than B. capriscus.
30628723	1	29	theme	glycoconjugates	220:234	arg1	localization					193:204	The localization	189:204	The localization of intestinal glycoconjugates of the blunthead pufferfish Sphoeroides pachygaster and the grey triggerfish Balistes capriscus from the north-western Ionian Sea	189:364	The localization of intestinal glycoconjugates of the blunthead pufferfish Sphoeroides pachygaster and the grey triggerfish Balistes capriscus from the north-western Ionian Sea was analysed by histochemical methods (PAS, AB pH 2.5, HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA) to assess how evolutionary loss of a functional stomach in S. pachygaster affects intestinal secretions relative to the B. capriscus, which retains the plesiomorphic gastric condition.
30628723	3	30	theme	GalNAc	832:837	arg1	residuals					850:858	GalNAc and GlcNAc residuals	832:858	GalNAc and GlcNAc residuals	832:858	GalNAc and GlcNAc residuals were present in both, but GalNAc residuals in S. pachygaster were subterminal to sialic acid.
30628723	1	31	from	loss	558:561	arg1	pachygaster					593:603	S. pachygaster	590:603	S. pachygaster	590:603	The localization of intestinal glycoconjugates of the blunthead pufferfish Sphoeroides pachygaster and the grey triggerfish Balistes capriscus from the north-western Ionian Sea was analysed by histochemical methods (PAS, AB pH 2.5, HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA) to assess how evolutionary loss of a functional stomach in S. pachygaster affects intestinal secretions relative to the B. capriscus, which retains the plesiomorphic gastric condition.
30628723	3	32	located	present	865:871	arg2	residuals					850:858	GalNAc and GlcNAc residuals	832:858	GalNAc and GlcNAc residuals	832:858	GalNAc and GlcNAc residuals were present in both, but GalNAc residuals in S. pachygaster were subterminal to sialic acid.
30628723	3	32	located	present	865:871	arg1	both					876:879	both	876:879	both	876:879	GalNAc and GlcNAc residuals were present in both, but GalNAc residuals in S. pachygaster were subterminal to sialic acid.
30628723	1	33	theme	relative	635:642	arg1	secretions					624:633	intestinal secretions	613:633	intestinal secretions relative to the B. capriscus	613:662	The localization of intestinal glycoconjugates of the blunthead pufferfish Sphoeroides pachygaster and the grey triggerfish Balistes capriscus from the north-western Ionian Sea was analysed by histochemical methods (PAS, AB pH 2.5, HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA) to assess how evolutionary loss of a functional stomach in S. pachygaster affects intestinal secretions relative to the B. capriscus, which retains the plesiomorphic gastric condition.
30628723	1	34	theme	north-western	341:353	arg1	Sea					362:364	the north-western Ionian Sea	337:364	the north-western Ionian Sea	337:364	The localization of intestinal glycoconjugates of the blunthead pufferfish Sphoeroides pachygaster and the grey triggerfish Balistes capriscus from the north-western Ionian Sea was analysed by histochemical methods (PAS, AB pH 2.5, HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA) to assess how evolutionary loss of a functional stomach in S. pachygaster affects intestinal secretions relative to the B. capriscus, which retains the plesiomorphic gastric condition.
30628723	2	35	theme	lower	746:750	arg1	content					752:758	a lower content	744:758	a lower content of acid mucins	744:773	Sphoeroides pachygaster had a lower content of acid mucins but more complex sialylation patterns than B. capriscus.
30628723	3	36	theme	GalNAc	886:891	arg1	residuals					893:901	GalNAc residuals	886:901	GalNAc residuals in S. pachygaster	886:919	GalNAc and GlcNAc residuals were present in both, but GalNAc residuals in S. pachygaster were subterminal to sialic acid.
30628723	0	37	theme	glycoconjugates	49:63	arg1	analysis					26:33	Comparative histochemical analysis	0:33	Comparative histochemical analysis of intestinal glycoconjugates in the blunthead pufferfish	0:91	Comparative histochemical analysis of intestinal glycoconjugates in the blunthead pufferfish Sphoeroides pachygaster and grey triggerfish Balistes capriscus (Teleostei: Tetraodontiformes).
30628723	1	38	theme	Ionian	355:360	arg1	Sea					362:364	the north-western Ionian Sea	337:364	the north-western Ionian Sea	337:364	The localization of intestinal glycoconjugates of the blunthead pufferfish Sphoeroides pachygaster and the grey triggerfish Balistes capriscus from the north-western Ionian Sea was analysed by histochemical methods (PAS, AB pH 2.5, HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA) to assess how evolutionary loss of a functional stomach in S. pachygaster affects intestinal secretions relative to the B. capriscus, which retains the plesiomorphic gastric condition.
30628723	2	39	contain	had	740:742	arg2	content					752:758	a lower content	744:758	a lower content of acid mucins	744:773	Sphoeroides pachygaster had a lower content of acid mucins but more complex sialylation patterns than B. capriscus.
30628723	2	39	contain	had	740:742	arg2	patterns					804:811	more complex sialylation patterns	779:811	more complex sialylation patterns	779:811	Sphoeroides pachygaster had a lower content of acid mucins but more complex sialylation patterns than B. capriscus.
30628723	2	39	contain	had	740:742	arg1	pachygaster					728:738	Sphoeroides pachygaster	716:738	Sphoeroides pachygaster	716:738	Sphoeroides pachygaster had a lower content of acid mucins but more complex sialylation patterns than B. capriscus.
30628723	3	40	attach	present	865:871	arg2	residuals					850:858	GalNAc and GlcNAc residuals	832:858	GalNAc and GlcNAc residuals	832:858	GalNAc and GlcNAc residuals were present in both, but GalNAc residuals in S. pachygaster were subterminal to sialic acid.
30628723	3	40	attach	present	865:871	arg1	both					876:879	both	876:879	both	876:879	GalNAc and GlcNAc residuals were present in both, but GalNAc residuals in S. pachygaster were subterminal to sialic acid.
30628723	1	41	theme	evolutionary	545:556	arg1	loss					558:561	evolutionary loss	545:561	evolutionary loss of a functional stomach in S. pachygaster	545:603	The localization of intestinal glycoconjugates of the blunthead pufferfish Sphoeroides pachygaster and the grey triggerfish Balistes capriscus from the north-western Ionian Sea was analysed by histochemical methods (PAS, AB pH 2.5, HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA) to assess how evolutionary loss of a functional stomach in S. pachygaster affects intestinal secretions relative to the B. capriscus, which retains the plesiomorphic gastric condition.
30628723	1	42	theme	blunthead	243:251	arg1	pachygaster					276:286	the blunthead pufferfish Sphoeroides pachygaster	239:286	the blunthead pufferfish Sphoeroides pachygaster	239:286	The localization of intestinal glycoconjugates of the blunthead pufferfish Sphoeroides pachygaster and the grey triggerfish Balistes capriscus from the north-western Ionian Sea was analysed by histochemical methods (PAS, AB pH 2.5, HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA) to assess how evolutionary loss of a functional stomach in S. pachygaster affects intestinal secretions relative to the B. capriscus, which retains the plesiomorphic gastric condition.
30628723	0	43	theme	intestinal	38:47	arg1	glycoconjugates					49:63	intestinal glycoconjugates	38:63	intestinal glycoconjugates	38:63	Comparative histochemical analysis of intestinal glycoconjugates in the blunthead pufferfish Sphoeroides pachygaster and grey triggerfish Balistes capriscus (Teleostei: Tetraodontiformes).
30628723	3	44	from	residuals	893:901	arg1	pachygaster					909:919	pachygaster	909:919	pachygaster	909:919	GalNAc and GlcNAc residuals were present in both, but GalNAc residuals in S. pachygaster were subterminal to sialic acid.
30628723	1	45	dep	LTA	526:528	arg1	AAA					514:516	HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA	421:528	AAA	514:516	The localization of intestinal glycoconjugates of the blunthead pufferfish Sphoeroides pachygaster and the grey triggerfish Balistes capriscus from the north-western Ionian Sea was analysed by histochemical methods (PAS, AB pH 2.5, HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA) to assess how evolutionary loss of a functional stomach in S. pachygaster affects intestinal secretions relative to the B. capriscus, which retains the plesiomorphic gastric condition.
30628723	1	45	dep	LTA	526:528	arg1	LFA					463:465	HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA	421:528	LFA	463:465	The localization of intestinal glycoconjugates of the blunthead pufferfish Sphoeroides pachygaster and the grey triggerfish Balistes capriscus from the north-western Ionian Sea was analysed by histochemical methods (PAS, AB pH 2.5, HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA) to assess how evolutionary loss of a functional stomach in S. pachygaster affects intestinal secretions relative to the B. capriscus, which retains the plesiomorphic gastric condition.
30628723	1	45	dep	LTA	526:528	arg1	PNA					488:490	HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA	421:528	PNA	488:490	The localization of intestinal glycoconjugates of the blunthead pufferfish Sphoeroides pachygaster and the grey triggerfish Balistes capriscus from the north-western Ionian Sea was analysed by histochemical methods (PAS, AB pH 2.5, HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA) to assess how evolutionary loss of a functional stomach in S. pachygaster affects intestinal secretions relative to the B. capriscus, which retains the plesiomorphic gastric condition.
30628723	1	45	dep	LTA	526:528	arg1	sialidase-PNA					493:505	HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA	421:528	sialidase-PNA	493:505	The localization of intestinal glycoconjugates of the blunthead pufferfish Sphoeroides pachygaster and the grey triggerfish Balistes capriscus from the north-western Ionian Sea was analysed by histochemical methods (PAS, AB pH 2.5, HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA) to assess how evolutionary loss of a functional stomach in S. pachygaster affects intestinal secretions relative to the B. capriscus, which retains the plesiomorphic gastric condition.
30628723	1	45	dep	LTA	526:528	arg1	UEA-I					519:523	HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA	421:528	UEA-I	519:523	The localization of intestinal glycoconjugates of the blunthead pufferfish Sphoeroides pachygaster and the grey triggerfish Balistes capriscus from the north-western Ionian Sea was analysed by histochemical methods (PAS, AB pH 2.5, HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA) to assess how evolutionary loss of a functional stomach in S. pachygaster affects intestinal secretions relative to the B. capriscus, which retains the plesiomorphic gastric condition.
30628723	1	45	dep	LTA	526:528	arg1	SBA					468:470	HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA	421:528	SBA	468:470	The localization of intestinal glycoconjugates of the blunthead pufferfish Sphoeroides pachygaster and the grey triggerfish Balistes capriscus from the north-western Ionian Sea was analysed by histochemical methods (PAS, AB pH 2.5, HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA) to assess how evolutionary loss of a functional stomach in S. pachygaster affects intestinal secretions relative to the B. capriscus, which retains the plesiomorphic gastric condition.
30628723	1	45	dep	LTA	526:528	arg1	sialidase-SBA					473:485	HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA	421:528	sialidase-SBA	473:485	The localization of intestinal glycoconjugates of the blunthead pufferfish Sphoeroides pachygaster and the grey triggerfish Balistes capriscus from the north-western Ionian Sea was analysed by histochemical methods (PAS, AB pH 2.5, HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA) to assess how evolutionary loss of a functional stomach in S. pachygaster affects intestinal secretions relative to the B. capriscus, which retains the plesiomorphic gastric condition.
30628723	1	45	dep	LTA	526:528	arg1	ConA					508:511	HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA	421:528	ConA	508:511	The localization of intestinal glycoconjugates of the blunthead pufferfish Sphoeroides pachygaster and the grey triggerfish Balistes capriscus from the north-western Ionian Sea was analysed by histochemical methods (PAS, AB pH 2.5, HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA) to assess how evolutionary loss of a functional stomach in S. pachygaster affects intestinal secretions relative to the B. capriscus, which retains the plesiomorphic gastric condition.
30628723	1	46	theme	pufferfish	253:262	arg1	pachygaster					276:286	the blunthead pufferfish Sphoeroides pachygaster	239:286	the blunthead pufferfish Sphoeroides pachygaster	239:286	The localization of intestinal glycoconjugates of the blunthead pufferfish Sphoeroides pachygaster and the grey triggerfish Balistes capriscus from the north-western Ionian Sea was analysed by histochemical methods (PAS, AB pH 2.5, HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA) to assess how evolutionary loss of a functional stomach in S. pachygaster affects intestinal secretions relative to the B. capriscus, which retains the plesiomorphic gastric condition.
30628723	1	47	theme	plesiomorphic	683:695	arg1	condition					705:713	the plesiomorphic gastric condition	679:713	the plesiomorphic gastric condition	679:713	The localization of intestinal glycoconjugates of the blunthead pufferfish Sphoeroides pachygaster and the grey triggerfish Balistes capriscus from the north-western Ionian Sea was analysed by histochemical methods (PAS, AB pH 2.5, HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA) to assess how evolutionary loss of a functional stomach in S. pachygaster affects intestinal secretions relative to the B. capriscus, which retains the plesiomorphic gastric condition.
30628723	4	48	theme	galactosylated	980:993	arg1	residuals					995:1003	galactosylated residuals	980:1003	galactosylated residuals	980:1003	Balistes capriscus lacked galactosylated residuals and its enterocytes had a glycocalyx that differed in composition between the small intestine and the rectum and was missing from S. pachygaster.
30628723	1	49	theme	lectin	430:435	arg1	LTA					526:528	HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA	421:528	LTA	526:528	The localization of intestinal glycoconjugates of the blunthead pufferfish Sphoeroides pachygaster and the grey triggerfish Balistes capriscus from the north-western Ionian Sea was analysed by histochemical methods (PAS, AB pH 2.5, HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA) to assess how evolutionary loss of a functional stomach in S. pachygaster affects intestinal secretions relative to the B. capriscus, which retains the plesiomorphic gastric condition.
30628723	1	49	theme	lectin	430:435	arg1	PAS					405:407	PAS	405:407	PAS	405:407	The localization of intestinal glycoconjugates of the blunthead pufferfish Sphoeroides pachygaster and the grey triggerfish Balistes capriscus from the north-western Ionian Sea was analysed by histochemical methods (PAS, AB pH 2.5, HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA) to assess how evolutionary loss of a functional stomach in S. pachygaster affects intestinal secretions relative to the B. capriscus, which retains the plesiomorphic gastric condition.
30628723	1	50	dep	affects	605:611	arg1	retains					671:677	retains	671:677	retains the plesiomorphic gastric condition	671:713	The localization of intestinal glycoconjugates of the blunthead pufferfish Sphoeroides pachygaster and the grey triggerfish Balistes capriscus from the north-western Ionian Sea was analysed by histochemical methods (PAS, AB pH 2.5, HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA) to assess how evolutionary loss of a functional stomach in S. pachygaster affects intestinal secretions relative to the B. capriscus, which retains the plesiomorphic gastric condition.
30628723	2	51	theme	complex	784:790	arg1	patterns					804:811	more complex sialylation patterns	779:811	more complex sialylation patterns	779:811	Sphoeroides pachygaster had a lower content of acid mucins but more complex sialylation patterns than B. capriscus.
30628723	1	52	theme	binding	437:443	arg1	LTA					526:528	HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA	421:528	LTA	526:528	The localization of intestinal glycoconjugates of the blunthead pufferfish Sphoeroides pachygaster and the grey triggerfish Balistes capriscus from the north-western Ionian Sea was analysed by histochemical methods (PAS, AB pH 2.5, HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA) to assess how evolutionary loss of a functional stomach in S. pachygaster affects intestinal secretions relative to the B. capriscus, which retains the plesiomorphic gastric condition.
30628723	1	52	theme	binding	437:443	arg1	PAS					405:407	PAS	405:407	PAS	405:407	The localization of intestinal glycoconjugates of the blunthead pufferfish Sphoeroides pachygaster and the grey triggerfish Balistes capriscus from the north-western Ionian Sea was analysed by histochemical methods (PAS, AB pH 2.5, HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA) to assess how evolutionary loss of a functional stomach in S. pachygaster affects intestinal secretions relative to the B. capriscus, which retains the plesiomorphic gastric condition.
30628723	5	53	theme	findings	1199:1206	arg1	implications					1177:1188	Functional and ecological implications	1151:1188	Functional and ecological implications of these findings	1151:1206	Functional and ecological implications of these findings are discussed.
30628723	3	54	theme	sialic	941:946	arg1	acid					948:951	sialic acid	941:951	sialic acid	941:951	GalNAc and GlcNAc residuals were present in both, but GalNAc residuals in S. pachygaster were subterminal to sialic acid.
30628723	1	55	theme	Sphoeroides	264:274	arg1	pachygaster					276:286	the blunthead pufferfish Sphoeroides pachygaster	239:286	the blunthead pufferfish Sphoeroides pachygaster	239:286	The localization of intestinal glycoconjugates of the blunthead pufferfish Sphoeroides pachygaster and the grey triggerfish Balistes capriscus from the north-western Ionian Sea was analysed by histochemical methods (PAS, AB pH 2.5, HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA) to assess how evolutionary loss of a functional stomach in S. pachygaster affects intestinal secretions relative to the B. capriscus, which retains the plesiomorphic gastric condition.
30628723	3	56	from	present	865:871	arg1	both					876:879	both	876:879	both	876:879	GalNAc and GlcNAc residuals were present in both, but GalNAc residuals in S. pachygaster were subterminal to sialic acid.
30628723	1	57	theme	experiments	445:455	arg1	LTA					526:528	HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA	421:528	LTA	526:528	The localization of intestinal glycoconjugates of the blunthead pufferfish Sphoeroides pachygaster and the grey triggerfish Balistes capriscus from the north-western Ionian Sea was analysed by histochemical methods (PAS, AB pH 2.5, HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA) to assess how evolutionary loss of a functional stomach in S. pachygaster affects intestinal secretions relative to the B. capriscus, which retains the plesiomorphic gastric condition.
30628723	1	57	theme	experiments	445:455	arg1	PAS					405:407	PAS	405:407	PAS	405:407	The localization of intestinal glycoconjugates of the blunthead pufferfish Sphoeroides pachygaster and the grey triggerfish Balistes capriscus from the north-western Ionian Sea was analysed by histochemical methods (PAS, AB pH 2.5, HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA) to assess how evolutionary loss of a functional stomach in S. pachygaster affects intestinal secretions relative to the B. capriscus, which retains the plesiomorphic gastric condition.
30628723	3	58	from	both	876:879	arg1	present					865:871	present	865:871	present	865:871	GalNAc and GlcNAc residuals were present in both, but GalNAc residuals in S. pachygaster were subterminal to sialic acid.
30628723	4	59	theme	small	1083:1087	arg1	intestine					1089:1097	the small intestine	1079:1097	the small intestine	1079:1097	Balistes capriscus lacked galactosylated residuals and its enterocytes had a glycocalyx that differed in composition between the small intestine and the rectum and was missing from S. pachygaster.
30628723	1	60	theme	pachygaster	276:286	arg1	localization					193:204	The localization	189:204	The localization of intestinal glycoconjugates of the blunthead pufferfish Sphoeroides pachygaster and the grey triggerfish Balistes capriscus from the north-western Ionian Sea	189:364	The localization of intestinal glycoconjugates of the blunthead pufferfish Sphoeroides pachygaster and the grey triggerfish Balistes capriscus from the north-western Ionian Sea was analysed by histochemical methods (PAS, AB pH 2.5, HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA) to assess how evolutionary loss of a functional stomach in S. pachygaster affects intestinal secretions relative to the B. capriscus, which retains the plesiomorphic gastric condition.
30628723	1	61	theme	functional	568:577	arg1	stomach					579:585	a functional stomach	566:585	a functional stomach in S. pachygaster	566:603	The localization of intestinal glycoconjugates of the blunthead pufferfish Sphoeroides pachygaster and the grey triggerfish Balistes capriscus from the north-western Ionian Sea was analysed by histochemical methods (PAS, AB pH 2.5, HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA) to assess how evolutionary loss of a functional stomach in S. pachygaster affects intestinal secretions relative to the B. capriscus, which retains the plesiomorphic gastric condition.
30628723	0	62	theme	blunthead	72:80	arg1	pufferfish					82:91	the blunthead pufferfish	68:91	the blunthead pufferfish	68:91	Comparative histochemical analysis of intestinal glycoconjugates in the blunthead pufferfish Sphoeroides pachygaster and grey triggerfish Balistes capriscus (Teleostei: Tetraodontiformes).
30628723	1	63	theme	histochemical	382:394	arg1	methods					396:402	histochemical methods	382:402	histochemical methods	382:402	The localization of intestinal glycoconjugates of the blunthead pufferfish Sphoeroides pachygaster and the grey triggerfish Balistes capriscus from the north-western Ionian Sea was analysed by histochemical methods (PAS, AB pH 2.5, HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA) to assess how evolutionary loss of a functional stomach in S. pachygaster affects intestinal secretions relative to the B. capriscus, which retains the plesiomorphic gastric condition.
30628723	1	64	from	localization	193:204	arg1	Sea					362:364	the north-western Ionian Sea	337:364	the north-western Ionian Sea	337:364	The localization of intestinal glycoconjugates of the blunthead pufferfish Sphoeroides pachygaster and the grey triggerfish Balistes capriscus from the north-western Ionian Sea was analysed by histochemical methods (PAS, AB pH 2.5, HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA) to assess how evolutionary loss of a functional stomach in S. pachygaster affects intestinal secretions relative to the B. capriscus, which retains the plesiomorphic gastric condition.
30628723	0	65	dep	Teleostei	158:166	arg1	Tetraodontiformes					169:185	Tetraodontiformes	169:185	Teleostei: Tetraodontiformes	158:185	Comparative histochemical analysis of intestinal glycoconjugates in the blunthead pufferfish Sphoeroides pachygaster and grey triggerfish Balistes capriscus (Teleostei: Tetraodontiformes).
30628723	1	66	theme	stomach	579:585	arg1	loss					558:561	evolutionary loss	545:561	evolutionary loss of a functional stomach in S. pachygaster	545:603	The localization of intestinal glycoconjugates of the blunthead pufferfish Sphoeroides pachygaster and the grey triggerfish Balistes capriscus from the north-western Ionian Sea was analysed by histochemical methods (PAS, AB pH 2.5, HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA) to assess how evolutionary loss of a functional stomach in S. pachygaster affects intestinal secretions relative to the B. capriscus, which retains the plesiomorphic gastric condition.
31832844	2	0	theme	Gram-stain-negative	101:119	arg1	PVNT-B20T					206:214	strain ChDC PVNT-B20T	194:214	strain ChDC PVNT-B20T	194:214	A Gram-stain-negative, facultative anaerobic, spore-forming, motile, and rod-shaped bacterium, strain ChDC PVNT-B20T, was isolated from the human subgingival dental plaque of a gingivitis lesion.
31832844	2	0	theme	Gram-stain-negative	101:119	arg1	bacterium					183:191	A Gram-stain-negative, facultative anaerobic, spore-forming, motile, and rod-shaped bacterium	99:191	A Gram-stain-negative, facultative anaerobic, spore-forming, motile, and rod-shaped bacterium	99:191	A Gram-stain-negative, facultative anaerobic, spore-forming, motile, and rod-shaped bacterium, strain ChDC PVNT-B20T, was isolated from the human subgingival dental plaque of a gingivitis lesion.
31832844	13	1	theme	Paenibacillus	1669:1681	arg1	sp					1690:1691	the name Paenibacillus oralis sp	1660:1691	the name Paenibacillus oralis sp	1660:1691	Based on these results, strain ChDC PVNT-B20T is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus oralis sp.
31832844	4	2	theme	%	547:547	arg1	23593T					535:540	Paenibacillus faecis DSM 23593T	510:540	Paenibacillus faecis DSM 23593T (97.7% similarity)	510:559	BLAST analysis of 16S rDNA sequence of the strain displayed high identity to those of Paenibacillus faecis DSM 23593T (97.7% similarity) and Paenibacillus macerans ATCC 8244T (97.6% similarity).
31832844	4	2	theme	%	547:547	arg1	similarity					549:558	97.7% similarity	543:558	97.7% similarity	543:558	BLAST analysis of 16S rDNA sequence of the strain displayed high identity to those of Paenibacillus faecis DSM 23593T (97.7% similarity) and Paenibacillus macerans ATCC 8244T (97.6% similarity).
31832844	6	3	theme	51.3 mol	725:732	arg1	content					699:705	The DNA G+C content	687:705	The DNA G+C content of the strain	687:719	The DNA G+C content of the strain was 51.3 mol%.
31832844	6	3	theme	51.3 mol	725:732	arg1	%					733:733	51.3 mol%	725:733	51.3 mol%	725:733	The DNA G+C content of the strain was 51.3 mol%.
31832844	7	4	theme	ChDC	786:789	arg1	PVNT-B20T					791:799	strain ChDC PVNT-B20T	779:799	strain ChDC PVNT-B20T	779:799	Average nucleotide identity values between strain ChDC PVNT-B20T and P. faecis DSM 23593T or P. macerans ATCC 8244T were 75.71% and 91.5%, respectively.
31832844	12	5	theme	unidentified	1504:1515	arg1	L1-L3					1525:1529	L1-L3	1525:1529	L1-L3	1525:1529	Minor polar lipids were one unidentified aminolipid (AL), one unidentified phospholipid (PL), and three unidentified lipids (L1-L3).
31832844	12	5	theme	unidentified	1504:1515	arg1	lipids					1517:1522	three unidentified lipids	1498:1522	three unidentified lipids (L1-L3)	1498:1530	Minor polar lipids were one unidentified aminolipid (AL), one unidentified phospholipid (PL), and three unidentified lipids (L1-L3).
31832844	4	6	theme	%	604:604	arg1	8244T					593:597	Paenibacillus macerans ATCC 8244T	565:597	Paenibacillus macerans ATCC 8244T (97.6% similarity)	565:616	BLAST analysis of 16S rDNA sequence of the strain displayed high identity to those of Paenibacillus faecis DSM 23593T (97.7% similarity) and Paenibacillus macerans ATCC 8244T (97.6% similarity).
31832844	4	6	theme	%	604:604	arg1	similarity					606:615	97.6% similarity	600:615	97.6% similarity	600:615	BLAST analysis of 16S rDNA sequence of the strain displayed high identity to those of Paenibacillus faecis DSM 23593T (97.7% similarity) and Paenibacillus macerans ATCC 8244T (97.6% similarity).
31832844	6	7	theme	strain	714:719	arg1	content					699:705	The DNA G+C content	687:705	The DNA G+C content of the strain	687:719	The DNA G+C content of the strain was 51.3 mol%.
31832844	6	7	theme	strain	714:719	arg1	%					733:733	51.3 mol%	725:733	51.3 mol%	725:733	The DNA G+C content of the strain was 51.3 mol%.
31832844	2	8	theme	dental	257:262	arg1	plaque					264:269	the human subgingival dental plaque	235:269	the human subgingival dental plaque of a gingivitis lesion	235:292	A Gram-stain-negative, facultative anaerobic, spore-forming, motile, and rod-shaped bacterium, strain ChDC PVNT-B20T, was isolated from the human subgingival dental plaque of a gingivitis lesion.
31832844	8	9	theme	distance	906:913	arg1	values					915:920	Genome-to-genome distance values	889:920	Genome-to-genome distance values between strain ChDC PVNT-B20T and P. faecis DSM 23593T or P. macerans ATCC 8244T	889:1001	Genome-to-genome distance values between strain ChDC PVNT-B20T and P. faecis DSM 23593T or P. macerans ATCC 8244T were 21.6% (19.3-24.0%) and 44.9% (42.3-47.4%), respectively.
31832844	9	10	theme	cellular	1071:1078	arg1	acids					1086:1090	Major cellular fatty acids	1065:1090	Major cellular fatty acids of strain ChDC PVNT-B20T	1065:1115	Major cellular fatty acids of strain ChDC PVNT-B20T were anteiso-C15:0 (43.4%), C16:0 (16.6%), iso-C16:0 (14.4%), and anteiso-C17:0 (12.4%).
31832844	7	11	theme	identity	755:762	arg1	values					764:769	Average nucleotide identity values	736:769	Average nucleotide identity values between strain ChDC PVNT-B20T and P. faecis DSM 23593T or P. macerans ATCC 8244T	736:850	Average nucleotide identity values between strain ChDC PVNT-B20T and P. faecis DSM 23593T or P. macerans ATCC 8244T were 75.71% and 91.5%, respectively.
31832844	4	12	theme	ATCC	588:591	arg1	8244T					593:597	Paenibacillus macerans ATCC 8244T	565:597	Paenibacillus macerans ATCC 8244T (97.6% similarity)	565:616	BLAST analysis of 16S rDNA sequence of the strain displayed high identity to those of Paenibacillus faecis DSM 23593T (97.7% similarity) and Paenibacillus macerans ATCC 8244T (97.6% similarity).
31832844	4	12	theme	ATCC	588:591	arg1	similarity					606:615	97.6% similarity	600:615	97.6% similarity	600:615	BLAST analysis of 16S rDNA sequence of the strain displayed high identity to those of Paenibacillus faecis DSM 23593T (97.7% similarity) and Paenibacillus macerans ATCC 8244T (97.6% similarity).
31832844	15	13	theme	3021T = JCM	1750:1760	arg1	PVNT-B20T					1732:1740	ChDC PVNT-B20T	1727:1740	ChDC PVNT-B20T (= KCOM 3021T = JCM 33462 T)	1727:1769	Type strain is ChDC PVNT-B20T (= KCOM 3021T = JCM 33462 T).
31832844	15	13	theme	3021T = JCM	1750:1760	arg1	33462 T					1762:1768	= KCOM 3021T = JCM 33462 T	1743:1768	= KCOM 3021T = JCM 33462 T	1743:1768	Type strain is ChDC PVNT-B20T (= KCOM 3021T = JCM 33462 T).
31832844	5	14	theme	ChDC	642:645	arg1	PVNT-B20T					647:655	strain ChDC PVNT-B20T	635:655	strain ChDC PVNT-B20T	635:655	Draft genome of strain ChDC PVNT-B20T was composed of 8,112,407 bp.
31832844	2	15	theme	gingivitis	276:285	arg1	lesion					287:292	a gingivitis lesion	274:292	a gingivitis lesion	274:292	A Gram-stain-negative, facultative anaerobic, spore-forming, motile, and rod-shaped bacterium, strain ChDC PVNT-B20T, was isolated from the human subgingival dental plaque of a gingivitis lesion.
31832844	7	16	theme	Average	736:742	arg1	values					764:769	Average nucleotide identity values	736:769	Average nucleotide identity values between strain ChDC PVNT-B20T and P. faecis DSM 23593T or P. macerans ATCC 8244T	736:850	Average nucleotide identity values between strain ChDC PVNT-B20T and P. faecis DSM 23593T or P. macerans ATCC 8244T were 75.71% and 91.5%, respectively.
31832844	9	17	theme	strain	1095:1100	arg1	PVNT-B20T					1107:1115	strain ChDC PVNT-B20T	1095:1115	strain ChDC PVNT-B20T	1095:1115	Major cellular fatty acids of strain ChDC PVNT-B20T were anteiso-C15:0 (43.4%), C16:0 (16.6%), iso-C16:0 (14.4%), and anteiso-C17:0 (12.4%).
31832844	4	18	theme	BLAST	424:428	arg1	analysis					430:437	BLAST analysis	424:437	BLAST analysis of 16S rDNA sequence of the strain	424:472	BLAST analysis of 16S rDNA sequence of the strain displayed high identity to those of Paenibacillus faecis DSM 23593T (97.7% similarity) and Paenibacillus macerans ATCC 8244T (97.6% similarity).
31832844	5	19	theme	Draft	619:623	arg1	genome					625:630	Draft genome	619:630	Draft genome of strain ChDC PVNT-B20T	619:655	Draft genome of strain ChDC PVNT-B20T was composed of 8,112,407 bp.
31832844	9	20	theme	PVNT-B20T	1107:1115	arg1	acids					1086:1090	Major cellular fatty acids	1065:1090	Major cellular fatty acids of strain ChDC PVNT-B20T	1065:1115	Major cellular fatty acids of strain ChDC PVNT-B20T were anteiso-C15:0 (43.4%), C16:0 (16.6%), iso-C16:0 (14.4%), and anteiso-C17:0 (12.4%).
31832844	13	21	theme	genus	1629:1633	arg1	species					1614:1620	a novel species	1606:1620	a novel species of the genus Paenibacillus, for which the name Paenibacillus oralis sp	1606:1691	Based on these results, strain ChDC PVNT-B20T is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus oralis sp.
31832844	2	22	theme	human	239:243	arg1	plaque					264:269	the human subgingival dental plaque	235:269	the human subgingival dental plaque of a gingivitis lesion	235:292	A Gram-stain-negative, facultative anaerobic, spore-forming, motile, and rod-shaped bacterium, strain ChDC PVNT-B20T, was isolated from the human subgingival dental plaque of a gingivitis lesion.
31832844	11	23	theme	Major	1267:1271	arg1	lipids					1279:1284	Major polar lipids	1267:1284	Major polar lipids of the strain	1267:1298	Major polar lipids of the strain were phosphatidylglycerol (PG), diphosphatidylglycerol (DPG), and one unidentified glycolipid (GL).
31832844	13	24	theme	novel	1608:1612	arg1	species					1614:1620	a novel species	1606:1620	a novel species of the genus Paenibacillus, for which the name Paenibacillus oralis sp	1606:1691	Based on these results, strain ChDC PVNT-B20T is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus oralis sp.
31832844	8	25	theme	ChDC	937:940	arg1	PVNT-B20T					942:950	strain ChDC PVNT-B20T	930:950	strain ChDC PVNT-B20T	930:950	Genome-to-genome distance values between strain ChDC PVNT-B20T and P. faecis DSM 23593T or P. macerans ATCC 8244T were 21.6% (19.3-24.0%) and 44.9% (42.3-47.4%), respectively.
31832844	2	26	attach	isolated	221:228	arg2	PVNT-B20T					206:214	strain ChDC PVNT-B20T	194:214	strain ChDC PVNT-B20T	194:214	A Gram-stain-negative, facultative anaerobic, spore-forming, motile, and rod-shaped bacterium, strain ChDC PVNT-B20T, was isolated from the human subgingival dental plaque of a gingivitis lesion.
31832844	2	26	attach	isolated	221:228	arg2	bacterium					183:191	A Gram-stain-negative, facultative anaerobic, spore-forming, motile, and rod-shaped bacterium	99:191	A Gram-stain-negative, facultative anaerobic, spore-forming, motile, and rod-shaped bacterium	99:191	A Gram-stain-negative, facultative anaerobic, spore-forming, motile, and rod-shaped bacterium, strain ChDC PVNT-B20T, was isolated from the human subgingival dental plaque of a gingivitis lesion.
31832844	2	26	attach	isolated	221:228	arg1	plaque					264:269	the human subgingival dental plaque	235:269	the human subgingival dental plaque of a gingivitis lesion	235:292	A Gram-stain-negative, facultative anaerobic, spore-forming, motile, and rod-shaped bacterium, strain ChDC PVNT-B20T, was isolated from the human subgingival dental plaque of a gingivitis lesion.
31832844	3	27	theme	RNA	344:346	arg1	rDNA					358:361	16S rDNA	354:361	16S rDNA	354:361	Phylogenetic analysis based on the 16S ribosomal RNA gene (16S rDNA) showed that the strain belonged to the genus Paenibacillus.
31832844	3	27	theme	RNA	344:346	arg1	gene					348:351	the 16S ribosomal RNA gene	326:351	the 16S ribosomal RNA gene (16S rDNA)	326:362	Phylogenetic analysis based on the 16S ribosomal RNA gene (16S rDNA) showed that the strain belonged to the genus Paenibacillus.
31832844	4	28	theme	strain	467:472	arg1	sequence					451:458	16S rDNA sequence	442:458	16S rDNA sequence of the strain	442:472	BLAST analysis of 16S rDNA sequence of the strain displayed high identity to those of Paenibacillus faecis DSM 23593T (97.7% similarity) and Paenibacillus macerans ATCC 8244T (97.6% similarity).
31832844	10	29	theme	respiratory	1215:1225	arg1	quinone					1227:1233	The sole respiratory quinone	1206:1233	The sole respiratory quinone of the strain	1206:1247	The sole respiratory quinone of the strain was menaqinone-7.
31832844	10	29	theme	respiratory	1215:1225	arg1	menaqinone-7					1253:1264	menaqinone-7	1253:1264	menaqinone-7	1253:1264	The sole respiratory quinone of the strain was menaqinone-7.
31832844	1	30	theme	Subgingival	51:61	arg1	Plaque					70:75	Human Subgingival Dental Plaque	45:75	Human Subgingival Dental Plaque of Gingivitis Lesion	45:96	nov., Isolated from Human Subgingival Dental Plaque of Gingivitis Lesion.
31832844	3	31	theme	16S	330:332	arg1	rDNA					358:361	16S rDNA	354:361	16S rDNA	354:361	Phylogenetic analysis based on the 16S ribosomal RNA gene (16S rDNA) showed that the strain belonged to the genus Paenibacillus.
31832844	3	31	theme	16S	330:332	arg1	gene					348:351	the 16S ribosomal RNA gene	326:351	the 16S ribosomal RNA gene (16S rDNA)	326:362	Phylogenetic analysis based on the 16S ribosomal RNA gene (16S rDNA) showed that the strain belonged to the genus Paenibacillus.
31832844	12	32	theme	unidentified	1428:1439	arg1	aminolipid					1441:1450	one unidentified aminolipid	1424:1450	one unidentified aminolipid (AL)	1424:1455	Minor polar lipids were one unidentified aminolipid (AL), one unidentified phospholipid (PL), and three unidentified lipids (L1-L3).
31832844	4	33	theme	rDNA	446:449	arg1	sequence					451:458	16S rDNA sequence	442:458	16S rDNA sequence of the strain	442:472	BLAST analysis of 16S rDNA sequence of the strain displayed high identity to those of Paenibacillus faecis DSM 23593T (97.7% similarity) and Paenibacillus macerans ATCC 8244T (97.6% similarity).
31832844	10	34	theme	strain	1242:1247	arg1	quinone					1227:1233	The sole respiratory quinone	1206:1233	The sole respiratory quinone of the strain	1206:1247	The sole respiratory quinone of the strain was menaqinone-7.
31832844	10	34	theme	strain	1242:1247	arg1	menaqinone-7					1253:1264	menaqinone-7	1253:1264	menaqinone-7	1253:1264	The sole respiratory quinone of the strain was menaqinone-7.
31832844	0	35	theme	Paenibacillus	0:12	arg1	sp					21:22	Paenibacillus oralis sp	0:22	Paenibacillus oralis sp.	0:23	Paenibacillus oralis sp.
31832844	7	36	theme	ATCC	841:844	arg1	8244T					846:850	P. macerans ATCC 8244T	829:850	P. macerans ATCC 8244T	829:850	Average nucleotide identity values between strain ChDC PVNT-B20T and P. faecis DSM 23593T or P. macerans ATCC 8244T were 75.71% and 91.5%, respectively.
31832844	1	37	theme	Gingivitis	80:89	arg1	Lesion					91:96	Gingivitis Lesion	80:96	Gingivitis Lesion	80:96	nov., Isolated from Human Subgingival Dental Plaque of Gingivitis Lesion.
31832844	13	38	theme	strain	1557:1562	arg1	PVNT-B20T					1569:1577	strain ChDC PVNT-B20T	1557:1577	strain ChDC PVNT-B20T	1557:1577	Based on these results, strain ChDC PVNT-B20T is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus oralis sp.
31832844	8	39	theme	P.	980:981	arg1	8244T					997:1001	P. macerans ATCC 8244T	980:1001	P. macerans ATCC 8244T	980:1001	Genome-to-genome distance values between strain ChDC PVNT-B20T and P. faecis DSM 23593T or P. macerans ATCC 8244T were 21.6% (19.3-24.0%) and 44.9% (42.3-47.4%), respectively.
31832844	12	40	theme	polar	1406:1410	arg1	lipids					1412:1417	Minor polar lipids	1400:1417	Minor polar lipids	1400:1417	Minor polar lipids were one unidentified aminolipid (AL), one unidentified phospholipid (PL), and three unidentified lipids (L1-L3).
31832844	4	41	theme	faecis	524:529	arg1	23593T					535:540	Paenibacillus faecis DSM 23593T	510:540	Paenibacillus faecis DSM 23593T (97.7% similarity)	510:559	BLAST analysis of 16S rDNA sequence of the strain displayed high identity to those of Paenibacillus faecis DSM 23593T (97.7% similarity) and Paenibacillus macerans ATCC 8244T (97.6% similarity).
31832844	4	41	theme	faecis	524:529	arg1	similarity					549:558	97.7% similarity	543:558	97.7% similarity	543:558	BLAST analysis of 16S rDNA sequence of the strain displayed high identity to those of Paenibacillus faecis DSM 23593T (97.7% similarity) and Paenibacillus macerans ATCC 8244T (97.6% similarity).
31832844	15	42	theme	Type	1712:1715	arg1	strain					1717:1722	Type strain	1712:1722	Type strain	1712:1722	Type strain is ChDC PVNT-B20T (= KCOM 3021T = JCM 33462 T).
31832844	3	43	theme	Paenibacillus	409:421	arg1	genus					403:407	the genus Paenibacillus	399:421	the genus Paenibacillus	399:421	Phylogenetic analysis based on the 16S ribosomal RNA gene (16S rDNA) showed that the strain belonged to the genus Paenibacillus.
31832844	8	44	theme	ATCC	992:995	arg1	8244T					997:1001	P. macerans ATCC 8244T	980:1001	P. macerans ATCC 8244T	980:1001	Genome-to-genome distance values between strain ChDC PVNT-B20T and P. faecis DSM 23593T or P. macerans ATCC 8244T were 21.6% (19.3-24.0%) and 44.9% (42.3-47.4%), respectively.
31832844	4	45	theme	high	484:487	arg1	identity					489:496	high identity	484:496	high identity to those of Paenibacillus faecis DSM 23593T (97.7% similarity) and Paenibacillus macerans ATCC 8244T (97.6% similarity)	484:616	BLAST analysis of 16S rDNA sequence of the strain displayed high identity to those of Paenibacillus faecis DSM 23593T (97.7% similarity) and Paenibacillus macerans ATCC 8244T (97.6% similarity).
31832844	2	46	theme	motile	160:165	arg1	PVNT-B20T					206:214	strain ChDC PVNT-B20T	194:214	strain ChDC PVNT-B20T	194:214	A Gram-stain-negative, facultative anaerobic, spore-forming, motile, and rod-shaped bacterium, strain ChDC PVNT-B20T, was isolated from the human subgingival dental plaque of a gingivitis lesion.
31832844	2	46	theme	motile	160:165	arg1	bacterium					183:191	A Gram-stain-negative, facultative anaerobic, spore-forming, motile, and rod-shaped bacterium	99:191	A Gram-stain-negative, facultative anaerobic, spore-forming, motile, and rod-shaped bacterium	99:191	A Gram-stain-negative, facultative anaerobic, spore-forming, motile, and rod-shaped bacterium, strain ChDC PVNT-B20T, was isolated from the human subgingival dental plaque of a gingivitis lesion.
31832844	6	47	theme	DNA	691:693	arg1	content					699:705	The DNA G+C content	687:705	The DNA G+C content of the strain	687:719	The DNA G+C content of the strain was 51.3 mol%.
31832844	6	47	theme	DNA	691:693	arg1	%					733:733	51.3 mol%	725:733	51.3 mol%	725:733	The DNA G+C content of the strain was 51.3 mol%.
31832844	2	48	theme	strain	194:199	arg1	PVNT-B20T					206:214	strain ChDC PVNT-B20T	194:214	strain ChDC PVNT-B20T	194:214	A Gram-stain-negative, facultative anaerobic, spore-forming, motile, and rod-shaped bacterium, strain ChDC PVNT-B20T, was isolated from the human subgingival dental plaque of a gingivitis lesion.
31832844	2	48	theme	strain	194:199	arg1	bacterium					183:191	A Gram-stain-negative, facultative anaerobic, spore-forming, motile, and rod-shaped bacterium	99:191	A Gram-stain-negative, facultative anaerobic, spore-forming, motile, and rod-shaped bacterium	99:191	A Gram-stain-negative, facultative anaerobic, spore-forming, motile, and rod-shaped bacterium, strain ChDC PVNT-B20T, was isolated from the human subgingival dental plaque of a gingivitis lesion.
31832844	7	49	theme	macerans	832:839	arg1	8244T					846:850	P. macerans ATCC 8244T	829:850	P. macerans ATCC 8244T	829:850	Average nucleotide identity values between strain ChDC PVNT-B20T and P. faecis DSM 23593T or P. macerans ATCC 8244T were 75.71% and 91.5%, respectively.
31832844	7	50	theme	DSM	815:817	arg1	23593T					819:824	P. faecis DSM 23593T	805:824	P. faecis DSM 23593T	805:824	Average nucleotide identity values between strain ChDC PVNT-B20T and P. faecis DSM 23593T or P. macerans ATCC 8244T were 75.71% and 91.5%, respectively.
31832844	8	51	dep	P.	956:957	arg1	faecis					959:964	faecis	959:964	faecis	959:964	Genome-to-genome distance values between strain ChDC PVNT-B20T and P. faecis DSM 23593T or P. macerans ATCC 8244T were 21.6% (19.3-24.0%) and 44.9% (42.3-47.4%), respectively.
31832844	7	52	dep	P.	805:806	arg1	faecis					808:813	faecis	808:813	faecis	808:813	Average nucleotide identity values between strain ChDC PVNT-B20T and P. faecis DSM 23593T or P. macerans ATCC 8244T were 75.71% and 91.5%, respectively.
31832844	13	53	theme	oralis	1683:1688	arg1	sp					1690:1691	the name Paenibacillus oralis sp	1660:1691	the name Paenibacillus oralis sp	1660:1691	Based on these results, strain ChDC PVNT-B20T is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus oralis sp.
31832844	7	54	theme	P.	805:806	arg1	23593T					819:824	P. faecis DSM 23593T	805:824	P. faecis DSM 23593T	805:824	Average nucleotide identity values between strain ChDC PVNT-B20T and P. faecis DSM 23593T or P. macerans ATCC 8244T were 75.71% and 91.5%, respectively.
31832844	8	55	theme	Genome-to-genome	889:904	arg1	values					915:920	Genome-to-genome distance values	889:920	Genome-to-genome distance values between strain ChDC PVNT-B20T and P. faecis DSM 23593T or P. macerans ATCC 8244T	889:1001	Genome-to-genome distance values between strain ChDC PVNT-B20T and P. faecis DSM 23593T or P. macerans ATCC 8244T were 21.6% (19.3-24.0%) and 44.9% (42.3-47.4%), respectively.
31832844	4	56	theme	97.7	543:546	arg1	%					547:547	%	547:547	%	547:547	BLAST analysis of 16S rDNA sequence of the strain displayed high identity to those of Paenibacillus faecis DSM 23593T (97.7% similarity) and Paenibacillus macerans ATCC 8244T (97.6% similarity).
31832844	2	57	theme	spore-forming	145:157	arg1	PVNT-B20T					206:214	strain ChDC PVNT-B20T	194:214	strain ChDC PVNT-B20T	194:214	A Gram-stain-negative, facultative anaerobic, spore-forming, motile, and rod-shaped bacterium, strain ChDC PVNT-B20T, was isolated from the human subgingival dental plaque of a gingivitis lesion.
31832844	2	57	theme	spore-forming	145:157	arg1	bacterium					183:191	A Gram-stain-negative, facultative anaerobic, spore-forming, motile, and rod-shaped bacterium	99:191	A Gram-stain-negative, facultative anaerobic, spore-forming, motile, and rod-shaped bacterium	99:191	A Gram-stain-negative, facultative anaerobic, spore-forming, motile, and rod-shaped bacterium, strain ChDC PVNT-B20T, was isolated from the human subgingival dental plaque of a gingivitis lesion.
31832844	12	58	dep	aminolipid	1441:1450	arg1	AL					1453:1454	AL	1453:1454	AL	1453:1454	Minor polar lipids were one unidentified aminolipid (AL), one unidentified phospholipid (PL), and three unidentified lipids (L1-L3).
31832844	4	59	theme	97.6	600:603	arg1	%					604:604	%	604:604	%	604:604	BLAST analysis of 16S rDNA sequence of the strain displayed high identity to those of Paenibacillus faecis DSM 23593T (97.7% similarity) and Paenibacillus macerans ATCC 8244T (97.6% similarity).
31832844	2	60	theme	anaerobic	134:142	arg1	PVNT-B20T					206:214	strain ChDC PVNT-B20T	194:214	strain ChDC PVNT-B20T	194:214	A Gram-stain-negative, facultative anaerobic, spore-forming, motile, and rod-shaped bacterium, strain ChDC PVNT-B20T, was isolated from the human subgingival dental plaque of a gingivitis lesion.
31832844	2	60	theme	anaerobic	134:142	arg1	bacterium					183:191	A Gram-stain-negative, facultative anaerobic, spore-forming, motile, and rod-shaped bacterium	99:191	A Gram-stain-negative, facultative anaerobic, spore-forming, motile, and rod-shaped bacterium	99:191	A Gram-stain-negative, facultative anaerobic, spore-forming, motile, and rod-shaped bacterium, strain ChDC PVNT-B20T, was isolated from the human subgingival dental plaque of a gingivitis lesion.
31832844	9	61	theme	Major	1065:1069	arg1	acids					1086:1090	Major cellular fatty acids	1065:1090	Major cellular fatty acids of strain ChDC PVNT-B20T	1065:1115	Major cellular fatty acids of strain ChDC PVNT-B20T were anteiso-C15:0 (43.4%), C16:0 (16.6%), iso-C16:0 (14.4%), and anteiso-C17:0 (12.4%).
31832844	7	62	theme	strain	779:784	arg1	PVNT-B20T					791:799	strain ChDC PVNT-B20T	779:799	strain ChDC PVNT-B20T	779:799	Average nucleotide identity values between strain ChDC PVNT-B20T and P. faecis DSM 23593T or P. macerans ATCC 8244T were 75.71% and 91.5%, respectively.
31832844	8	63	theme	strain	930:935	arg1	PVNT-B20T					942:950	strain ChDC PVNT-B20T	930:950	strain ChDC PVNT-B20T	930:950	Genome-to-genome distance values between strain ChDC PVNT-B20T and P. faecis DSM 23593T or P. macerans ATCC 8244T were 21.6% (19.3-24.0%) and 44.9% (42.3-47.4%), respectively.
31832844	5	64	theme	strain	635:640	arg1	PVNT-B20T					647:655	strain ChDC PVNT-B20T	635:655	strain ChDC PVNT-B20T	635:655	Draft genome of strain ChDC PVNT-B20T was composed of 8,112,407 bp.
31832844	9	65	theme	fatty	1080:1084	arg1	acids					1086:1090	Major cellular fatty acids	1065:1090	Major cellular fatty acids of strain ChDC PVNT-B20T	1065:1115	Major cellular fatty acids of strain ChDC PVNT-B20T were anteiso-C15:0 (43.4%), C16:0 (16.6%), iso-C16:0 (14.4%), and anteiso-C17:0 (12.4%).
31832844	2	66	theme	lesion	287:292	arg1	plaque					264:269	the human subgingival dental plaque	235:269	the human subgingival dental plaque of a gingivitis lesion	235:292	A Gram-stain-negative, facultative anaerobic, spore-forming, motile, and rod-shaped bacterium, strain ChDC PVNT-B20T, was isolated from the human subgingival dental plaque of a gingivitis lesion.
31832844	4	67	theme	macerans	579:586	arg1	8244T					593:597	Paenibacillus macerans ATCC 8244T	565:597	Paenibacillus macerans ATCC 8244T (97.6% similarity)	565:616	BLAST analysis of 16S rDNA sequence of the strain displayed high identity to those of Paenibacillus faecis DSM 23593T (97.7% similarity) and Paenibacillus macerans ATCC 8244T (97.6% similarity).
31832844	4	67	theme	macerans	579:586	arg1	similarity					606:615	97.6% similarity	600:615	97.6% similarity	600:615	BLAST analysis of 16S rDNA sequence of the strain displayed high identity to those of Paenibacillus faecis DSM 23593T (97.7% similarity) and Paenibacillus macerans ATCC 8244T (97.6% similarity).
31832844	5	68	theme	PVNT-B20T	647:655	arg1	genome					625:630	Draft genome	619:630	Draft genome of strain ChDC PVNT-B20T	619:655	Draft genome of strain ChDC PVNT-B20T was composed of 8,112,407 bp.
31832844	7	69	theme	nucleotide	744:753	arg1	values					764:769	Average nucleotide identity values	736:769	Average nucleotide identity values between strain ChDC PVNT-B20T and P. faecis DSM 23593T or P. macerans ATCC 8244T	736:850	Average nucleotide identity values between strain ChDC PVNT-B20T and P. faecis DSM 23593T or P. macerans ATCC 8244T were 75.71% and 91.5%, respectively.
31832844	9	70	theme	ChDC	1102:1105	arg1	PVNT-B20T					1107:1115	strain ChDC PVNT-B20T	1095:1115	strain ChDC PVNT-B20T	1095:1115	Major cellular fatty acids of strain ChDC PVNT-B20T were anteiso-C15:0 (43.4%), C16:0 (16.6%), iso-C16:0 (14.4%), and anteiso-C17:0 (12.4%).
31832844	3	71	theme	Phylogenetic	295:306	arg1	analysis					308:315	Phylogenetic analysis	295:315	Phylogenetic analysis based on the 16S ribosomal RNA gene (16S rDNA)	295:362	Phylogenetic analysis based on the 16S ribosomal RNA gene (16S rDNA) showed that the strain belonged to the genus Paenibacillus.
31832844	11	72	theme	polar	1273:1277	arg1	lipids					1279:1284	Major polar lipids	1267:1284	Major polar lipids of the strain	1267:1298	Major polar lipids of the strain were phosphatidylglycerol (PG), diphosphatidylglycerol (DPG), and one unidentified glycolipid (GL).
31832844	13	73	theme	name	1664:1667	arg1	sp					1690:1691	the name Paenibacillus oralis sp	1660:1691	the name Paenibacillus oralis sp	1660:1691	Based on these results, strain ChDC PVNT-B20T is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus oralis sp.
31832844	2	74	theme	subgingival	245:255	arg1	plaque					264:269	the human subgingival dental plaque	235:269	the human subgingival dental plaque of a gingivitis lesion	235:292	A Gram-stain-negative, facultative anaerobic, spore-forming, motile, and rod-shaped bacterium, strain ChDC PVNT-B20T, was isolated from the human subgingival dental plaque of a gingivitis lesion.
31832844	13	75	theme	Paenibacillus	1635:1647	arg1	genus					1629:1633	the genus Paenibacillus	1625:1647	the genus Paenibacillus	1625:1647	Based on these results, strain ChDC PVNT-B20T is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus oralis sp.
31832844	12	76	theme	unidentified	1462:1473	arg1	PL					1489:1490	PL	1489:1490	PL	1489:1490	Minor polar lipids were one unidentified aminolipid (AL), one unidentified phospholipid (PL), and three unidentified lipids (L1-L3).
31832844	12	76	theme	unidentified	1462:1473	arg1	phospholipid					1475:1486	one unidentified phospholipid	1458:1486	one unidentified phospholipid (PL)	1458:1491	Minor polar lipids were one unidentified aminolipid (AL), one unidentified phospholipid (PL), and three unidentified lipids (L1-L3).
31832844	11	77	theme	strain	1293:1298	arg1	lipids					1279:1284	Major polar lipids	1267:1284	Major polar lipids of the strain	1267:1298	Major polar lipids of the strain were phosphatidylglycerol (PG), diphosphatidylglycerol (DPG), and one unidentified glycolipid (GL).
31832844	8	78	theme	P.	956:957	arg1	23593T					970:975	P. faecis DSM 23593T	956:975	P. faecis DSM 23593T	956:975	Genome-to-genome distance values between strain ChDC PVNT-B20T and P. faecis DSM 23593T or P. macerans ATCC 8244T were 21.6% (19.3-24.0%) and 44.9% (42.3-47.4%), respectively.
31832844	1	79	theme	Human	45:49	arg1	Plaque					70:75	Human Subgingival Dental Plaque	45:75	Human Subgingival Dental Plaque of Gingivitis Lesion	45:96	nov., Isolated from Human Subgingival Dental Plaque of Gingivitis Lesion.
31832844	4	80	theme	16S	442:444	arg1	sequence					451:458	16S rDNA sequence	442:458	16S rDNA sequence of the strain	442:472	BLAST analysis of 16S rDNA sequence of the strain displayed high identity to those of Paenibacillus faecis DSM 23593T (97.7% similarity) and Paenibacillus macerans ATCC 8244T (97.6% similarity).
31832844	10	81	theme	sole	1210:1213	arg1	quinone					1227:1233	The sole respiratory quinone	1206:1233	The sole respiratory quinone of the strain	1206:1247	The sole respiratory quinone of the strain was menaqinone-7.
31832844	10	81	theme	sole	1210:1213	arg1	menaqinone-7					1253:1264	menaqinone-7	1253:1264	menaqinone-7	1253:1264	The sole respiratory quinone of the strain was menaqinone-7.
31832844	8	82	theme	DSM	966:968	arg1	23593T					970:975	P. faecis DSM 23593T	956:975	P. faecis DSM 23593T	956:975	Genome-to-genome distance values between strain ChDC PVNT-B20T and P. faecis DSM 23593T or P. macerans ATCC 8244T were 21.6% (19.3-24.0%) and 44.9% (42.3-47.4%), respectively.
31832844	1	83	theme	Dental	63:68	arg1	Plaque					70:75	Human Subgingival Dental Plaque	45:75	Human Subgingival Dental Plaque of Gingivitis Lesion	45:96	nov., Isolated from Human Subgingival Dental Plaque of Gingivitis Lesion.
31832844	3	84	theme	ribosomal	334:342	arg1	rDNA					358:361	16S rDNA	354:361	16S rDNA	354:361	Phylogenetic analysis based on the 16S ribosomal RNA gene (16S rDNA) showed that the strain belonged to the genus Paenibacillus.
31832844	3	84	theme	ribosomal	334:342	arg1	gene					348:351	the 16S ribosomal RNA gene	326:351	the 16S ribosomal RNA gene (16S rDNA)	326:362	Phylogenetic analysis based on the 16S ribosomal RNA gene (16S rDNA) showed that the strain belonged to the genus Paenibacillus.
31832844	4	85	theme	sequence	451:458	arg1	analysis					430:437	BLAST analysis	424:437	BLAST analysis of 16S rDNA sequence of the strain	424:472	BLAST analysis of 16S rDNA sequence of the strain displayed high identity to those of Paenibacillus faecis DSM 23593T (97.7% similarity) and Paenibacillus macerans ATCC 8244T (97.6% similarity).
31832844	0	86	theme	oralis	14:19	arg1	sp					21:22	Paenibacillus oralis sp	0:22	Paenibacillus oralis sp.	0:23	Paenibacillus oralis sp.
31832844	15	87	theme	= KCOM	1743:1748	arg1	PVNT-B20T					1732:1740	ChDC PVNT-B20T	1727:1740	ChDC PVNT-B20T (= KCOM 3021T = JCM 33462 T)	1727:1769	Type strain is ChDC PVNT-B20T (= KCOM 3021T = JCM 33462 T).
31832844	15	87	theme	= KCOM	1743:1748	arg1	33462 T					1762:1768	= KCOM 3021T = JCM 33462 T	1743:1768	= KCOM 3021T = JCM 33462 T	1743:1768	Type strain is ChDC PVNT-B20T (= KCOM 3021T = JCM 33462 T).
31832844	4	88	theme	Paenibacillus	510:522	arg1	23593T					535:540	Paenibacillus faecis DSM 23593T	510:540	Paenibacillus faecis DSM 23593T (97.7% similarity)	510:559	BLAST analysis of 16S rDNA sequence of the strain displayed high identity to those of Paenibacillus faecis DSM 23593T (97.7% similarity) and Paenibacillus macerans ATCC 8244T (97.6% similarity).
31832844	4	88	theme	Paenibacillus	510:522	arg1	similarity					549:558	97.7% similarity	543:558	97.7% similarity	543:558	BLAST analysis of 16S rDNA sequence of the strain displayed high identity to those of Paenibacillus faecis DSM 23593T (97.7% similarity) and Paenibacillus macerans ATCC 8244T (97.6% similarity).
31832844	8	89	theme	macerans	983:990	arg1	8244T					997:1001	P. macerans ATCC 8244T	980:1001	P. macerans ATCC 8244T	980:1001	Genome-to-genome distance values between strain ChDC PVNT-B20T and P. faecis DSM 23593T or P. macerans ATCC 8244T were 21.6% (19.3-24.0%) and 44.9% (42.3-47.4%), respectively.
31832844	1	90	theme	Lesion	91:96	arg1	Plaque					70:75	Human Subgingival Dental Plaque	45:75	Human Subgingival Dental Plaque of Gingivitis Lesion	45:96	nov., Isolated from Human Subgingival Dental Plaque of Gingivitis Lesion.
31832844	4	91	theme	DSM	531:533	arg1	23593T					535:540	Paenibacillus faecis DSM 23593T	510:540	Paenibacillus faecis DSM 23593T (97.7% similarity)	510:559	BLAST analysis of 16S rDNA sequence of the strain displayed high identity to those of Paenibacillus faecis DSM 23593T (97.7% similarity) and Paenibacillus macerans ATCC 8244T (97.6% similarity).
31832844	4	91	theme	DSM	531:533	arg1	similarity					549:558	97.7% similarity	543:558	97.7% similarity	543:558	BLAST analysis of 16S rDNA sequence of the strain displayed high identity to those of Paenibacillus faecis DSM 23593T (97.7% similarity) and Paenibacillus macerans ATCC 8244T (97.6% similarity).
31832844	15	92	theme	ChDC	1727:1730	arg1	PVNT-B20T					1732:1740	ChDC PVNT-B20T	1727:1740	ChDC PVNT-B20T (= KCOM 3021T = JCM 33462 T)	1727:1769	Type strain is ChDC PVNT-B20T (= KCOM 3021T = JCM 33462 T).
31832844	15	92	theme	ChDC	1727:1730	arg1	33462 T					1762:1768	= KCOM 3021T = JCM 33462 T	1743:1768	= KCOM 3021T = JCM 33462 T	1743:1768	Type strain is ChDC PVNT-B20T (= KCOM 3021T = JCM 33462 T).
31832844	2	93	theme	rod-shaped	172:181	arg1	PVNT-B20T					206:214	strain ChDC PVNT-B20T	194:214	strain ChDC PVNT-B20T	194:214	A Gram-stain-negative, facultative anaerobic, spore-forming, motile, and rod-shaped bacterium, strain ChDC PVNT-B20T, was isolated from the human subgingival dental plaque of a gingivitis lesion.
31832844	2	93	theme	rod-shaped	172:181	arg1	bacterium					183:191	A Gram-stain-negative, facultative anaerobic, spore-forming, motile, and rod-shaped bacterium	99:191	A Gram-stain-negative, facultative anaerobic, spore-forming, motile, and rod-shaped bacterium	99:191	A Gram-stain-negative, facultative anaerobic, spore-forming, motile, and rod-shaped bacterium, strain ChDC PVNT-B20T, was isolated from the human subgingival dental plaque of a gingivitis lesion.
31832844	13	94	theme	ChDC	1564:1567	arg1	PVNT-B20T					1569:1577	strain ChDC PVNT-B20T	1557:1577	strain ChDC PVNT-B20T	1557:1577	Based on these results, strain ChDC PVNT-B20T is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus oralis sp.
31832844	12	95	theme	Minor	1400:1404	arg1	lipids					1412:1417	Minor polar lipids	1400:1417	Minor polar lipids	1400:1417	Minor polar lipids were one unidentified aminolipid (AL), one unidentified phospholipid (PL), and three unidentified lipids (L1-L3).
31832844	2	96	dep	Gram-stain-negative	101:119	arg1	facultative					122:132	facultative	122:132	facultative	122:132	A Gram-stain-negative, facultative anaerobic, spore-forming, motile, and rod-shaped bacterium, strain ChDC PVNT-B20T, was isolated from the human subgingival dental plaque of a gingivitis lesion.
31832844	6	97	theme	G+C	695:697	arg1	content					699:705	The DNA G+C content	687:705	The DNA G+C content of the strain	687:719	The DNA G+C content of the strain was 51.3 mol%.
31832844	6	97	theme	G+C	695:697	arg1	%					733:733	51.3 mol%	725:733	51.3 mol%	725:733	The DNA G+C content of the strain was 51.3 mol%.
31832844	2	98	theme	ChDC	201:204	arg1	PVNT-B20T					206:214	strain ChDC PVNT-B20T	194:214	strain ChDC PVNT-B20T	194:214	A Gram-stain-negative, facultative anaerobic, spore-forming, motile, and rod-shaped bacterium, strain ChDC PVNT-B20T, was isolated from the human subgingival dental plaque of a gingivitis lesion.
31832844	2	98	theme	ChDC	201:204	arg1	bacterium					183:191	A Gram-stain-negative, facultative anaerobic, spore-forming, motile, and rod-shaped bacterium	99:191	A Gram-stain-negative, facultative anaerobic, spore-forming, motile, and rod-shaped bacterium	99:191	A Gram-stain-negative, facultative anaerobic, spore-forming, motile, and rod-shaped bacterium, strain ChDC PVNT-B20T, was isolated from the human subgingival dental plaque of a gingivitis lesion.
31832844	7	99	theme	P.	829:830	arg1	8244T					846:850	P. macerans ATCC 8244T	829:850	P. macerans ATCC 8244T	829:850	Average nucleotide identity values between strain ChDC PVNT-B20T and P. faecis DSM 23593T or P. macerans ATCC 8244T were 75.71% and 91.5%, respectively.
31832844	4	100	theme	Paenibacillus	565:577	arg1	8244T					593:597	Paenibacillus macerans ATCC 8244T	565:597	Paenibacillus macerans ATCC 8244T (97.6% similarity)	565:616	BLAST analysis of 16S rDNA sequence of the strain displayed high identity to those of Paenibacillus faecis DSM 23593T (97.7% similarity) and Paenibacillus macerans ATCC 8244T (97.6% similarity).
31832844	4	100	theme	Paenibacillus	565:577	arg1	similarity					606:615	97.6% similarity	600:615	97.6% similarity	600:615	BLAST analysis of 16S rDNA sequence of the strain displayed high identity to those of Paenibacillus faecis DSM 23593T (97.7% similarity) and Paenibacillus macerans ATCC 8244T (97.6% similarity).
31832844	11	101	theme	unidentified	1370:1381	arg1	glycolipid					1383:1392	one unidentified glycolipid	1366:1392	one unidentified glycolipid (GL)	1366:1397	Major polar lipids of the strain were phosphatidylglycerol (PG), diphosphatidylglycerol (DPG), and one unidentified glycolipid (GL).
31832844	11	101	theme	unidentified	1370:1381	arg1	GL					1395:1396	GL	1395:1396	GL	1395:1396	Major polar lipids of the strain were phosphatidylglycerol (PG), diphosphatidylglycerol (DPG), and one unidentified glycolipid (GL).
30860460	9	0	theme	hybridization	1240:1252	arg1	value					1254:1258	The in silico DNA-DNA hybridization value	1218:1258	The in silico DNA-DNA hybridization value between strains X-1125T and X-1174	1218:1293	The in silico DNA-DNA hybridization value between strains X-1125T and X-1174 was 97.8 % (well above the species threshold), but their values were lower than the 70 % threshold with the three closely related type strains.
30860460	4	1	theme	%	678:678	arg1	33762T					665:670	Paraliobacillussediminis KCTC 33762T	635:670	Paraliobacillussediminis KCTC 33762T (98.4 % similarity)	635:690	Based on the results of 16S rRNA gene sequences, and phylogenetic and phylogenomic analyses, their nearest phylogenetic neighbours were Paraliobacillussediminis KCTC 33762T (98.4 % similarity), Paraliobacillusquinghaiensis CGMCC 1.6333T (96.9 %) and Paraliobacillusryukyuensis NBRC 100001T (95.9 %) while the 16S rRNA genes of strains X-1125T and X-1174 were highly similar (99.7 %) to each other.
30860460	4	1	theme	%	678:678	arg1	similarity					680:689	98.4 % similarity	673:689	98.4 % similarity	673:689	Based on the results of 16S rRNA gene sequences, and phylogenetic and phylogenomic analyses, their nearest phylogenetic neighbours were Paraliobacillussediminis KCTC 33762T (98.4 % similarity), Paraliobacillusquinghaiensis CGMCC 1.6333T (96.9 %) and Paraliobacillusryukyuensis NBRC 100001T (95.9 %) while the 16S rRNA genes of strains X-1125T and X-1174 were highly similar (99.7 %) to each other.
30860460	9	2	theme	in	1222:1223	arg1	hybridization					1240:1252	The in silico DNA-DNA hybridization	1218:1252	The in silico DNA-DNA hybridization value between strains X-1125T and X-1174	1218:1293	The in silico DNA-DNA hybridization value between strains X-1125T and X-1174 was 97.8 % (well above the species threshold), but their values were lower than the 70 % threshold with the three closely related type strains.
30860460	1	3	dep	bacterium	83:91	arg1	nov.					28:31	nov.	28:31	nov.	28:31	nov., a slightly halophilic and extremely halotolerant bacterium isolated from Tibetan antelope faeces.
30860460	13	4	theme	=DSM	1740:1743	arg1	X-1125T					1731:1737	X-1125T	1731:1737	X-1125T (=DSM 107811T=CGMCC 1.16464T)	1731:1767	The type strain is X-1125T (=DSM 107811T=CGMCC 1.16464T).
30860460	13	4	theme	=DSM	1740:1743	arg1	1.16464T					1759:1766	=DSM 107811T=CGMCC 1.16464T	1740:1766	=DSM 107811T=CGMCC 1.16464T	1740:1766	The type strain is X-1125T (=DSM 107811T=CGMCC 1.16464T).
30860460	4	5	theme	sequences	537:545	arg1	results					512:518	the results	508:518	the results of 16S rRNA gene sequences, and phylogenetic and phylogenomic analyses	508:589	Based on the results of 16S rRNA gene sequences, and phylogenetic and phylogenomic analyses, their nearest phylogenetic neighbours were Paraliobacillussediminis KCTC 33762T (98.4 % similarity), Paraliobacillusquinghaiensis CGMCC 1.6333T (96.9 %) and Paraliobacillusryukyuensis NBRC 100001T (95.9 %) while the 16S rRNA genes of strains X-1125T and X-1174 were highly similar (99.7 %) to each other.
30860460	9	6	dep	in	1222:1223	arg1	silico					1225:1230	silico	1225:1230	silico	1225:1230	The in silico DNA-DNA hybridization value between strains X-1125T and X-1174 was 97.8 % (well above the species threshold), but their values were lower than the 70 % threshold with the three closely related type strains.
30860460	4	7	theme	strains	826:832	arg1	genes					817:821	the 16S rRNA genes	804:821	the 16S rRNA genes of strains X-1125T and X-1174	804:851	Based on the results of 16S rRNA gene sequences, and phylogenetic and phylogenomic analyses, their nearest phylogenetic neighbours were Paraliobacillussediminis KCTC 33762T (98.4 % similarity), Paraliobacillusquinghaiensis CGMCC 1.6333T (96.9 %) and Paraliobacillusryukyuensis NBRC 100001T (95.9 %) while the 16S rRNA genes of strains X-1125T and X-1174 were highly similar (99.7 %) to each other.
30860460	4	7	theme	strains	826:832	arg1	similar					865:871	similar	865:871	similar	865:871	Based on the results of 16S rRNA gene sequences, and phylogenetic and phylogenomic analyses, their nearest phylogenetic neighbours were Paraliobacillussediminis KCTC 33762T (98.4 % similarity), Paraliobacillusquinghaiensis CGMCC 1.6333T (96.9 %) and Paraliobacillusryukyuensis NBRC 100001T (95.9 %) while the 16S rRNA genes of strains X-1125T and X-1174 were highly similar (99.7 %) to each other.
30860460	4	7	theme	strains	826:832	arg1	%					879:879	99.7 %	874:879	99.7 %	874:879	Based on the results of 16S rRNA gene sequences, and phylogenetic and phylogenomic analyses, their nearest phylogenetic neighbours were Paraliobacillussediminis KCTC 33762T (98.4 % similarity), Paraliobacillusquinghaiensis CGMCC 1.6333T (96.9 %) and Paraliobacillusryukyuensis NBRC 100001T (95.9 %) while the 16S rRNA genes of strains X-1125T and X-1174 were highly similar (99.7 %) to each other.
30860460	4	8	theme	98.4 	673:677	arg1	33762T					665:670	Paraliobacillussediminis KCTC 33762T	635:670	Paraliobacillussediminis KCTC 33762T (98.4 % similarity)	635:690	Based on the results of 16S rRNA gene sequences, and phylogenetic and phylogenomic analyses, their nearest phylogenetic neighbours were Paraliobacillussediminis KCTC 33762T (98.4 % similarity), Paraliobacillusquinghaiensis CGMCC 1.6333T (96.9 %) and Paraliobacillusryukyuensis NBRC 100001T (95.9 %) while the 16S rRNA genes of strains X-1125T and X-1174 were highly similar (99.7 %) to each other.
30860460	4	8	theme	98.4 	673:677	arg1	similarity					680:689	98.4 % similarity	673:689	98.4 % similarity	673:689	Based on the results of 16S rRNA gene sequences, and phylogenetic and phylogenomic analyses, their nearest phylogenetic neighbours were Paraliobacillussediminis KCTC 33762T (98.4 % similarity), Paraliobacillusquinghaiensis CGMCC 1.6333T (96.9 %) and Paraliobacillusryukyuensis NBRC 100001T (95.9 %) while the 16S rRNA genes of strains X-1125T and X-1174 were highly similar (99.7 %) to each other.
30860460	13	9	theme	type	1716:1719	arg1	X-1125T					1731:1737	X-1125T	1731:1737	X-1125T (=DSM 107811T=CGMCC 1.16464T)	1731:1767	The type strain is X-1125T (=DSM 107811T=CGMCC 1.16464T).
30860460	13	9	theme	type	1716:1719	arg1	strain					1721:1726	The type strain	1712:1726	The type strain	1712:1726	The type strain is X-1125T (=DSM 107811T=CGMCC 1.16464T).
30860460	4	10	theme	gene	532:535	arg1	sequences					537:545	16S rRNA gene sequences	523:545	16S rRNA gene sequences	523:545	Based on the results of 16S rRNA gene sequences, and phylogenetic and phylogenomic analyses, their nearest phylogenetic neighbours were Paraliobacillussediminis KCTC 33762T (98.4 % similarity), Paraliobacillusquinghaiensis CGMCC 1.6333T (96.9 %) and Paraliobacillusryukyuensis NBRC 100001T (95.9 %) while the 16S rRNA genes of strains X-1125T and X-1174 were highly similar (99.7 %) to each other.
30860460	11	11	theme	novel	1603:1607	arg1	species					1609:1615	a novel species	1601:1615	a novel species	1601:1615	Based on the presented data, strains X-1125T and X-1174 hereby represent a novel species of the genus Paraliobacillus, for which the name Paraliobacillus zengyii sp.
30860460	7	12	theme	cell	1052:1055	arg1	wall					1057:1060	The cell wall	1048:1060	The cell wall	1048:1060	The cell wall contained alanine, glycine, glutamic acid and meso-diaminopimelic acid.
30860460	10	13	contain	had	1466:1468	arg2	%					1491:1491	mol%	1488:1491	mol%	1488:1491	Strains X-1125T and X-1174 had DNA G+C contents (mol%) of 35.2 and 35.1 %, respectively.
30860460	10	13	contain	had	1466:1468	arg1	X-1174					1459:1464	X-1174	1459:1464	X-1174	1459:1464	Strains X-1125T and X-1174 had DNA G+C contents (mol%) of 35.2 and 35.1 %, respectively.
30860460	10	13	contain	had	1466:1468	arg1	Strains					1439:1445	Strains	1439:1445	Strains X-1125T and X-1174	1439:1464	Strains X-1125T and X-1174 had DNA G+C contents (mol%) of 35.2 and 35.1 %, respectively.
30860460	10	13	contain	had	1466:1468	arg2	G+C contents					1474:1485	DNA G+C contents	1470:1485	DNA G+C contents (mol%) of 35.2 and 35.1 %	1470:1511	Strains X-1125T and X-1174 had DNA G+C contents (mol%) of 35.2 and 35.1 %, respectively.
30860460	10	13	contain	had	1466:1468	arg1	X-1125T					1447:1453	X-1125T	1447:1453	X-1125T	1447:1453	Strains X-1125T and X-1174 had DNA G+C contents (mol%) of 35.2 and 35.1 %, respectively.
30860460	1	14	theme	halophilic	45:54	arg1	bacterium					83:91	a slightly halophilic and extremely halotolerant bacterium	34:91	a slightly halophilic and extremely halotolerant bacterium isolated from Tibetan antelope faeces	34:129	nov., a slightly halophilic and extremely halotolerant bacterium isolated from Tibetan antelope faeces.
30860460	2	15	theme	peritrichous	307:318	arg1	flagella					320:327	peritrichous flagella	307:327	peritrichous flagella	307:327	Two rod-shaped, slightly halophilic and extremely halotolerant bacterial strains (X-1125T and X-1174), which were Gram-stain-positive, facultatively anaerobic and motile with peritrichous flagella, were isolated from the faeces of Tibetan antelopes.
30860460	5	16	theme	polar	901:905	arg1	lipids					907:912	The polar lipids	897:912	The polar lipids	897:912	The polar lipids comprised diphosphatidylglycerol, two unidentified phospholipids and four unidentified lipids.
30860460	2	17	theme	halophilic	157:166	arg1	X-1174					226:231	X-1174	226:231	X-1174	226:231	Two rod-shaped, slightly halophilic and extremely halotolerant bacterial strains (X-1125T and X-1174), which were Gram-stain-positive, facultatively anaerobic and motile with peritrichous flagella, were isolated from the faeces of Tibetan antelopes.
30860460	2	17	theme	halophilic	157:166	arg1	X-1125T					214:220	X-1125T	214:220	X-1125T	214:220	Two rod-shaped, slightly halophilic and extremely halotolerant bacterial strains (X-1125T and X-1174), which were Gram-stain-positive, facultatively anaerobic and motile with peritrichous flagella, were isolated from the faeces of Tibetan antelopes.
30860460	2	17	theme	halophilic	157:166	arg1	strains					205:211	Two rod-shaped, slightly halophilic and extremely halotolerant bacterial strains	132:211	Two rod-shaped, slightly halophilic and extremely halotolerant bacterial strains (X-1125T and X-1174)	132:232	Two rod-shaped, slightly halophilic and extremely halotolerant bacterial strains (X-1125T and X-1174), which were Gram-stain-positive, facultatively anaerobic and motile with peritrichous flagella, were isolated from the faeces of Tibetan antelopes.
30860460	7	18	contain	contained	1062:1070	arg2	alanine					1072:1078	alanine	1072:1078	alanine	1072:1078	The cell wall contained alanine, glycine, glutamic acid and meso-diaminopimelic acid.
30860460	7	18	contain	contained	1062:1070	arg1	wall					1057:1060	The cell wall	1048:1060	The cell wall	1048:1060	The cell wall contained alanine, glycine, glutamic acid and meso-diaminopimelic acid.
30860460	7	18	contain	contained	1062:1070	arg2	acid					1128:1131	meso-diaminopimelic acid	1108:1131	meso-diaminopimelic acid	1108:1131	The cell wall contained alanine, glycine, glutamic acid and meso-diaminopimelic acid.
30860460	7	18	contain	contained	1062:1070	arg2	acid					1099:1102	glutamic acid	1090:1102	glutamic acid	1090:1102	The cell wall contained alanine, glycine, glutamic acid and meso-diaminopimelic acid.
30860460	7	18	contain	contained	1062:1070	arg2	glycine					1081:1087	glycine	1081:1087	glycine	1081:1087	The cell wall contained alanine, glycine, glutamic acid and meso-diaminopimelic acid.
30860460	2	19	theme	Tibetan	363:369	arg1	antelopes					371:379	Tibetan antelopes	363:379	Tibetan antelopes	363:379	Two rod-shaped, slightly halophilic and extremely halotolerant bacterial strains (X-1125T and X-1174), which were Gram-stain-positive, facultatively anaerobic and motile with peritrichous flagella, were isolated from the faeces of Tibetan antelopes.
30860460	11	20	theme	Paraliobacillus	1666:1680	arg1	sp					1690:1691	the name Paraliobacillus zengyii sp	1657:1691	the name Paraliobacillus zengyii sp	1657:1691	Based on the presented data, strains X-1125T and X-1174 hereby represent a novel species of the genus Paraliobacillus, for which the name Paraliobacillus zengyii sp.
30860460	10	21	theme	DNA	1470:1472	arg1	G+C contents					1474:1485	DNA G+C contents	1470:1485	DNA G+C contents (mol%) of 35.2 and 35.1 %	1470:1511	Strains X-1125T and X-1174 had DNA G+C contents (mol%) of 35.2 and 35.1 %, respectively.
30860460	10	21	theme	DNA	1470:1472	arg1	%					1491:1491	mol%	1488:1491	mol%	1488:1491	Strains X-1125T and X-1174 had DNA G+C contents (mol%) of 35.2 and 35.1 %, respectively.
30860460	4	22	theme	rRNA	812:815	arg1	genes					817:821	the 16S rRNA genes	804:821	the 16S rRNA genes of strains X-1125T and X-1174	804:851	Based on the results of 16S rRNA gene sequences, and phylogenetic and phylogenomic analyses, their nearest phylogenetic neighbours were Paraliobacillussediminis KCTC 33762T (98.4 % similarity), Paraliobacillusquinghaiensis CGMCC 1.6333T (96.9 %) and Paraliobacillusryukyuensis NBRC 100001T (95.9 %) while the 16S rRNA genes of strains X-1125T and X-1174 were highly similar (99.7 %) to each other.
30860460	4	22	theme	rRNA	812:815	arg1	similar					865:871	similar	865:871	similar	865:871	Based on the results of 16S rRNA gene sequences, and phylogenetic and phylogenomic analyses, their nearest phylogenetic neighbours were Paraliobacillussediminis KCTC 33762T (98.4 % similarity), Paraliobacillusquinghaiensis CGMCC 1.6333T (96.9 %) and Paraliobacillusryukyuensis NBRC 100001T (95.9 %) while the 16S rRNA genes of strains X-1125T and X-1174 were highly similar (99.7 %) to each other.
30860460	4	22	theme	rRNA	812:815	arg1	%					879:879	99.7 %	874:879	99.7 %	874:879	Based on the results of 16S rRNA gene sequences, and phylogenetic and phylogenomic analyses, their nearest phylogenetic neighbours were Paraliobacillussediminis KCTC 33762T (98.4 % similarity), Paraliobacillusquinghaiensis CGMCC 1.6333T (96.9 %) and Paraliobacillusryukyuensis NBRC 100001T (95.9 %) while the 16S rRNA genes of strains X-1125T and X-1174 were highly similar (99.7 %) to each other.
30860460	4	23	theme	Paraliobacillusryukyuensis	749:774	arg1	100001T					781:787	Paraliobacillusryukyuensis NBRC 100001T	749:787	Paraliobacillusryukyuensis NBRC 100001T (95.9 %)	749:796	Based on the results of 16S rRNA gene sequences, and phylogenetic and phylogenomic analyses, their nearest phylogenetic neighbours were Paraliobacillussediminis KCTC 33762T (98.4 % similarity), Paraliobacillusquinghaiensis CGMCC 1.6333T (96.9 %) and Paraliobacillusryukyuensis NBRC 100001T (95.9 %) while the 16S rRNA genes of strains X-1125T and X-1174 were highly similar (99.7 %) to each other.
30860460	4	23	theme	Paraliobacillusryukyuensis	749:774	arg1	%					795:795	95.9 %	790:795	95.9 %	790:795	Based on the results of 16S rRNA gene sequences, and phylogenetic and phylogenomic analyses, their nearest phylogenetic neighbours were Paraliobacillussediminis KCTC 33762T (98.4 % similarity), Paraliobacillusquinghaiensis CGMCC 1.6333T (96.9 %) and Paraliobacillusryukyuensis NBRC 100001T (95.9 %) while the 16S rRNA genes of strains X-1125T and X-1174 were highly similar (99.7 %) to each other.
30860460	11	24	dep	Paraliobacillus	1666:1680	arg1	zengyii					1682:1688	zengyii	1682:1688	zengyii	1682:1688	Based on the presented data, strains X-1125T and X-1174 hereby represent a novel species of the genus Paraliobacillus, for which the name Paraliobacillus zengyii sp.
30860460	9	25	theme	species	1322:1328	arg1	threshold					1330:1338	the species threshold	1318:1338	the species threshold	1318:1338	The in silico DNA-DNA hybridization value between strains X-1125T and X-1174 was 97.8 % (well above the species threshold), but their values were lower than the 70 % threshold with the three closely related type strains.
30860460	4	26	dep	strains	826:832	arg1	X-1125T					834:840	X-1125T	834:840	X-1125T	834:840	Based on the results of 16S rRNA gene sequences, and phylogenetic and phylogenomic analyses, their nearest phylogenetic neighbours were Paraliobacillussediminis KCTC 33762T (98.4 % similarity), Paraliobacillusquinghaiensis CGMCC 1.6333T (96.9 %) and Paraliobacillusryukyuensis NBRC 100001T (95.9 %) while the 16S rRNA genes of strains X-1125T and X-1174 were highly similar (99.7 %) to each other.
30860460	4	26	dep	strains	826:832	arg1	strains					826:832	strains X-1125T and X-1174	826:851	strains X-1125T and X-1174	826:851	Based on the results of 16S rRNA gene sequences, and phylogenetic and phylogenomic analyses, their nearest phylogenetic neighbours were Paraliobacillussediminis KCTC 33762T (98.4 % similarity), Paraliobacillusquinghaiensis CGMCC 1.6333T (96.9 %) and Paraliobacillusryukyuensis NBRC 100001T (95.9 %) while the 16S rRNA genes of strains X-1125T and X-1174 were highly similar (99.7 %) to each other.
30860460	4	26	dep	strains	826:832	arg1	X-1174					846:851	X-1174	846:851	X-1174	846:851	Based on the results of 16S rRNA gene sequences, and phylogenetic and phylogenomic analyses, their nearest phylogenetic neighbours were Paraliobacillussediminis KCTC 33762T (98.4 % similarity), Paraliobacillusquinghaiensis CGMCC 1.6333T (96.9 %) and Paraliobacillusryukyuensis NBRC 100001T (95.9 %) while the 16S rRNA genes of strains X-1125T and X-1174 were highly similar (99.7 %) to each other.
30860460	2	27	attach	isolated	335:342	arg2	X-1125T					214:220	X-1125T	214:220	X-1125T	214:220	Two rod-shaped, slightly halophilic and extremely halotolerant bacterial strains (X-1125T and X-1174), which were Gram-stain-positive, facultatively anaerobic and motile with peritrichous flagella, were isolated from the faeces of Tibetan antelopes.
30860460	2	27	attach	isolated	335:342	arg1	faeces					353:358	the faeces	349:358	the faeces of Tibetan antelopes	349:379	Two rod-shaped, slightly halophilic and extremely halotolerant bacterial strains (X-1125T and X-1174), which were Gram-stain-positive, facultatively anaerobic and motile with peritrichous flagella, were isolated from the faeces of Tibetan antelopes.
30860460	2	27	attach	isolated	335:342	arg2	strains					205:211	Two rod-shaped, slightly halophilic and extremely halotolerant bacterial strains	132:211	Two rod-shaped, slightly halophilic and extremely halotolerant bacterial strains (X-1125T and X-1174)	132:232	Two rod-shaped, slightly halophilic and extremely halotolerant bacterial strains (X-1125T and X-1174), which were Gram-stain-positive, facultatively anaerobic and motile with peritrichous flagella, were isolated from the faeces of Tibetan antelopes.
30860460	2	27	attach	isolated	335:342	arg2	X-1174					226:231	X-1174	226:231	X-1174	226:231	Two rod-shaped, slightly halophilic and extremely halotolerant bacterial strains (X-1125T and X-1174), which were Gram-stain-positive, facultatively anaerobic and motile with peritrichous flagella, were isolated from the faeces of Tibetan antelopes.
30860460	4	28	theme	16S	808:810	arg1	genes					817:821	the 16S rRNA genes	804:821	the 16S rRNA genes of strains X-1125T and X-1174	804:851	Based on the results of 16S rRNA gene sequences, and phylogenetic and phylogenomic analyses, their nearest phylogenetic neighbours were Paraliobacillussediminis KCTC 33762T (98.4 % similarity), Paraliobacillusquinghaiensis CGMCC 1.6333T (96.9 %) and Paraliobacillusryukyuensis NBRC 100001T (95.9 %) while the 16S rRNA genes of strains X-1125T and X-1174 were highly similar (99.7 %) to each other.
30860460	4	28	theme	16S	808:810	arg1	similar					865:871	similar	865:871	similar	865:871	Based on the results of 16S rRNA gene sequences, and phylogenetic and phylogenomic analyses, their nearest phylogenetic neighbours were Paraliobacillussediminis KCTC 33762T (98.4 % similarity), Paraliobacillusquinghaiensis CGMCC 1.6333T (96.9 %) and Paraliobacillusryukyuensis NBRC 100001T (95.9 %) while the 16S rRNA genes of strains X-1125T and X-1174 were highly similar (99.7 %) to each other.
30860460	4	28	theme	16S	808:810	arg1	%					879:879	99.7 %	874:879	99.7 %	874:879	Based on the results of 16S rRNA gene sequences, and phylogenetic and phylogenomic analyses, their nearest phylogenetic neighbours were Paraliobacillussediminis KCTC 33762T (98.4 % similarity), Paraliobacillusquinghaiensis CGMCC 1.6333T (96.9 %) and Paraliobacillusryukyuensis NBRC 100001T (95.9 %) while the 16S rRNA genes of strains X-1125T and X-1174 were highly similar (99.7 %) to each other.
30860460	13	29	theme	107811T=CGMCC	1745:1757	arg1	X-1125T					1731:1737	X-1125T	1731:1737	X-1125T (=DSM 107811T=CGMCC 1.16464T)	1731:1767	The type strain is X-1125T (=DSM 107811T=CGMCC 1.16464T).
30860460	13	29	theme	107811T=CGMCC	1745:1757	arg1	1.16464T					1759:1766	=DSM 107811T=CGMCC 1.16464T	1740:1766	=DSM 107811T=CGMCC 1.16464T	1740:1766	The type strain is X-1125T (=DSM 107811T=CGMCC 1.16464T).
30860460	1	30	theme	halotolerant	70:81	arg1	bacterium					83:91	a slightly halophilic and extremely halotolerant bacterium	34:91	a slightly halophilic and extremely halotolerant bacterium isolated from Tibetan antelope faeces	34:129	nov., a slightly halophilic and extremely halotolerant bacterium isolated from Tibetan antelope faeces.
30860460	0	31	theme	zengyii	16:22	arg1	sp					24:25	Paraliobacillus zengyii sp	0:25	Paraliobacillus zengyii sp.	0:26	Paraliobacillus zengyii sp.
30860460	2	32	theme	rod-shaped	136:145	arg1	X-1174					226:231	X-1174	226:231	X-1174	226:231	Two rod-shaped, slightly halophilic and extremely halotolerant bacterial strains (X-1125T and X-1174), which were Gram-stain-positive, facultatively anaerobic and motile with peritrichous flagella, were isolated from the faeces of Tibetan antelopes.
30860460	2	32	theme	rod-shaped	136:145	arg1	X-1125T					214:220	X-1125T	214:220	X-1125T	214:220	Two rod-shaped, slightly halophilic and extremely halotolerant bacterial strains (X-1125T and X-1174), which were Gram-stain-positive, facultatively anaerobic and motile with peritrichous flagella, were isolated from the faeces of Tibetan antelopes.
30860460	2	32	theme	rod-shaped	136:145	arg1	strains					205:211	Two rod-shaped, slightly halophilic and extremely halotolerant bacterial strains	132:211	Two rod-shaped, slightly halophilic and extremely halotolerant bacterial strains (X-1125T and X-1174)	132:232	Two rod-shaped, slightly halophilic and extremely halotolerant bacterial strains (X-1125T and X-1174), which were Gram-stain-positive, facultatively anaerobic and motile with peritrichous flagella, were isolated from the faeces of Tibetan antelopes.
30860460	11	33	theme	name	1661:1664	arg1	sp					1690:1691	the name Paraliobacillus zengyii sp	1657:1691	the name Paraliobacillus zengyii sp	1657:1691	Based on the presented data, strains X-1125T and X-1174 hereby represent a novel species of the genus Paraliobacillus, for which the name Paraliobacillus zengyii sp.
30860460	9	34	theme	type	1425:1428	arg1	strains					1430:1436	the three closely related type strains	1399:1436	the three closely related type strains	1399:1436	The in silico DNA-DNA hybridization value between strains X-1125T and X-1174 was 97.8 % (well above the species threshold), but their values were lower than the 70 % threshold with the three closely related type strains.
30860460	7	35	theme	meso-diaminopimelic	1108:1126	arg1	acid					1128:1131	meso-diaminopimelic acid	1108:1131	meso-diaminopimelic acid	1108:1131	The cell wall contained alanine, glycine, glutamic acid and meso-diaminopimelic acid.
30860460	4	36	theme	KCTC	660:663	arg1	33762T					665:670	Paraliobacillussediminis KCTC 33762T	635:670	Paraliobacillussediminis KCTC 33762T (98.4 % similarity)	635:690	Based on the results of 16S rRNA gene sequences, and phylogenetic and phylogenomic analyses, their nearest phylogenetic neighbours were Paraliobacillussediminis KCTC 33762T (98.4 % similarity), Paraliobacillusquinghaiensis CGMCC 1.6333T (96.9 %) and Paraliobacillusryukyuensis NBRC 100001T (95.9 %) while the 16S rRNA genes of strains X-1125T and X-1174 were highly similar (99.7 %) to each other.
30860460	4	36	theme	KCTC	660:663	arg1	similarity					680:689	98.4 % similarity	673:689	98.4 % similarity	673:689	Based on the results of 16S rRNA gene sequences, and phylogenetic and phylogenomic analyses, their nearest phylogenetic neighbours were Paraliobacillussediminis KCTC 33762T (98.4 % similarity), Paraliobacillusquinghaiensis CGMCC 1.6333T (96.9 %) and Paraliobacillusryukyuensis NBRC 100001T (95.9 %) while the 16S rRNA genes of strains X-1125T and X-1174 were highly similar (99.7 %) to each other.
30860460	0	37	theme	Paraliobacillus	0:14	arg1	sp					24:25	Paraliobacillus zengyii sp	0:25	Paraliobacillus zengyii sp.	0:26	Paraliobacillus zengyii sp.
30860460	11	38	theme	genus	1624:1628	arg1	Paraliobacillus					1630:1644	the genus Paraliobacillus	1620:1644	the genus Paraliobacillus	1620:1644	Based on the presented data, strains X-1125T and X-1174 hereby represent a novel species of the genus Paraliobacillus, for which the name Paraliobacillus zengyii sp.
30860460	4	39	theme	CGMCC	722:726	arg1	1.6333T					728:734	Paraliobacillusquinghaiensis CGMCC 1.6333T	693:734	Paraliobacillusquinghaiensis CGMCC 1.6333T (96.9 %)	693:743	Based on the results of 16S rRNA gene sequences, and phylogenetic and phylogenomic analyses, their nearest phylogenetic neighbours were Paraliobacillussediminis KCTC 33762T (98.4 % similarity), Paraliobacillusquinghaiensis CGMCC 1.6333T (96.9 %) and Paraliobacillusryukyuensis NBRC 100001T (95.9 %) while the 16S rRNA genes of strains X-1125T and X-1174 were highly similar (99.7 %) to each other.
30860460	4	39	theme	CGMCC	722:726	arg1	%					742:742	96.9 %	737:742	96.9 %	737:742	Based on the results of 16S rRNA gene sequences, and phylogenetic and phylogenomic analyses, their nearest phylogenetic neighbours were Paraliobacillussediminis KCTC 33762T (98.4 % similarity), Paraliobacillusquinghaiensis CGMCC 1.6333T (96.9 %) and Paraliobacillusryukyuensis NBRC 100001T (95.9 %) while the 16S rRNA genes of strains X-1125T and X-1174 were highly similar (99.7 %) to each other.
30860460	4	40	theme	Paraliobacillussediminis	635:658	arg1	33762T					665:670	Paraliobacillussediminis KCTC 33762T	635:670	Paraliobacillussediminis KCTC 33762T (98.4 % similarity)	635:690	Based on the results of 16S rRNA gene sequences, and phylogenetic and phylogenomic analyses, their nearest phylogenetic neighbours were Paraliobacillussediminis KCTC 33762T (98.4 % similarity), Paraliobacillusquinghaiensis CGMCC 1.6333T (96.9 %) and Paraliobacillusryukyuensis NBRC 100001T (95.9 %) while the 16S rRNA genes of strains X-1125T and X-1174 were highly similar (99.7 %) to each other.
30860460	4	40	theme	Paraliobacillussediminis	635:658	arg1	similarity					680:689	98.4 % similarity	673:689	98.4 % similarity	673:689	Based on the results of 16S rRNA gene sequences, and phylogenetic and phylogenomic analyses, their nearest phylogenetic neighbours were Paraliobacillussediminis KCTC 33762T (98.4 % similarity), Paraliobacillusquinghaiensis CGMCC 1.6333T (96.9 %) and Paraliobacillusryukyuensis NBRC 100001T (95.9 %) while the 16S rRNA genes of strains X-1125T and X-1174 were highly similar (99.7 %) to each other.
30860460	8	41	theme	fatty	1144:1148	arg1	anteiso-C15 					1168:1179	anteiso-C15 	1168:1179	anteiso-C15 	1168:1179	The major fatty acids (>9 %) were anteiso-C15 : 0, anteiso-C17 : 0 and C16 : 1ω11c.
30860460	8	41	theme	fatty	1144:1148	arg1	 0					1181:1182	 0	1181:1182	 0	1181:1182	The major fatty acids (>9 %) were anteiso-C15 : 0, anteiso-C17 : 0 and C16 : 1ω11c.
30860460	8	41	theme	fatty	1144:1148	arg1	acids					1150:1154	The major fatty acids	1134:1154	The major fatty acids (>9 %)	1134:1161	The major fatty acids (>9 %) were anteiso-C15 : 0, anteiso-C17 : 0 and C16 : 1ω11c.
30860460	8	41	theme	fatty	1144:1148	arg1	%					1160:1160	>9 %	1157:1160	>9 %	1157:1160	The major fatty acids (>9 %) were anteiso-C15 : 0, anteiso-C17 : 0 and C16 : 1ω11c.
30860460	5	42	theme	unidentified	952:963	arg1	phospholipids					965:977	two unidentified phospholipids	948:977	two unidentified phospholipids	948:977	The polar lipids comprised diphosphatidylglycerol, two unidentified phospholipids and four unidentified lipids.
30860460	5	42	theme	unidentified	952:963	arg1	diphosphatidylglycerol					924:945	diphosphatidylglycerol	924:945	diphosphatidylglycerol	924:945	The polar lipids comprised diphosphatidylglycerol, two unidentified phospholipids and four unidentified lipids.
30860460	10	43	dep	Strains	1439:1445	arg1	Strains					1439:1445	Strains	1439:1445	Strains X-1125T and X-1174	1439:1464	Strains X-1125T and X-1174 had DNA G+C contents (mol%) of 35.2 and 35.1 %, respectively.
30860460	10	43	dep	Strains	1439:1445	arg1	X-1174					1459:1464	X-1174	1459:1464	X-1174	1459:1464	Strains X-1125T and X-1174 had DNA G+C contents (mol%) of 35.2 and 35.1 %, respectively.
30860460	10	43	dep	Strains	1439:1445	arg1	X-1125T					1447:1453	X-1125T	1447:1453	X-1125T	1447:1453	Strains X-1125T and X-1174 had DNA G+C contents (mol%) of 35.2 and 35.1 %, respectively.
30860460	9	44	with	threshold	1384:1392	arg1	strains					1430:1436	the three closely related type strains	1399:1436	the three closely related type strains	1399:1436	The in silico DNA-DNA hybridization value between strains X-1125T and X-1174 was 97.8 % (well above the species threshold), but their values were lower than the 70 % threshold with the three closely related type strains.
30860460	3	45	theme	%	460:460	arg1	NaCl					468:471	3 % (w/v) NaCl	458:471	3 % (w/v) NaCl	458:471	Their optimal temperature, NaCl concentration and pH for growth were 28 °C, 3 % (w/v) NaCl and pH 7.5, respectively.
30860460	3	46	theme	optimal	388:394	arg1	temperature					396:406	Their optimal temperature	382:406	Their optimal temperature	382:406	Their optimal temperature, NaCl concentration and pH for growth were 28 °C, 3 % (w/v) NaCl and pH 7.5, respectively.
30860460	4	47	theme	rRNA	527:530	arg1	sequences					537:545	16S rRNA gene sequences	523:545	16S rRNA gene sequences	523:545	Based on the results of 16S rRNA gene sequences, and phylogenetic and phylogenomic analyses, their nearest phylogenetic neighbours were Paraliobacillussediminis KCTC 33762T (98.4 % similarity), Paraliobacillusquinghaiensis CGMCC 1.6333T (96.9 %) and Paraliobacillusryukyuensis NBRC 100001T (95.9 %) while the 16S rRNA genes of strains X-1125T and X-1174 were highly similar (99.7 %) to each other.
30860460	9	48	theme	related	1417:1423	arg1	strains					1430:1436	the three closely related type strains	1399:1436	the three closely related type strains	1399:1436	The in silico DNA-DNA hybridization value between strains X-1125T and X-1174 was 97.8 % (well above the species threshold), but their values were lower than the 70 % threshold with the three closely related type strains.
30860460	2	49	theme	bacterial	195:203	arg1	X-1174					226:231	X-1174	226:231	X-1174	226:231	Two rod-shaped, slightly halophilic and extremely halotolerant bacterial strains (X-1125T and X-1174), which were Gram-stain-positive, facultatively anaerobic and motile with peritrichous flagella, were isolated from the faeces of Tibetan antelopes.
30860460	2	49	theme	bacterial	195:203	arg1	X-1125T					214:220	X-1125T	214:220	X-1125T	214:220	Two rod-shaped, slightly halophilic and extremely halotolerant bacterial strains (X-1125T and X-1174), which were Gram-stain-positive, facultatively anaerobic and motile with peritrichous flagella, were isolated from the faeces of Tibetan antelopes.
30860460	2	49	theme	bacterial	195:203	arg1	strains					205:211	Two rod-shaped, slightly halophilic and extremely halotolerant bacterial strains	132:211	Two rod-shaped, slightly halophilic and extremely halotolerant bacterial strains (X-1125T and X-1174)	132:232	Two rod-shaped, slightly halophilic and extremely halotolerant bacterial strains (X-1125T and X-1174), which were Gram-stain-positive, facultatively anaerobic and motile with peritrichous flagella, were isolated from the faeces of Tibetan antelopes.
30860460	1	50	theme	Tibetan	107:113	arg1	faeces					124:129	Tibetan antelope faeces	107:129	Tibetan antelope faeces	107:129	nov., a slightly halophilic and extremely halotolerant bacterium isolated from Tibetan antelope faeces.
30860460	4	51	theme	16S	523:525	arg1	sequences					537:545	16S rRNA gene sequences	523:545	16S rRNA gene sequences	523:545	Based on the results of 16S rRNA gene sequences, and phylogenetic and phylogenomic analyses, their nearest phylogenetic neighbours were Paraliobacillussediminis KCTC 33762T (98.4 % similarity), Paraliobacillusquinghaiensis CGMCC 1.6333T (96.9 %) and Paraliobacillusryukyuensis NBRC 100001T (95.9 %) while the 16S rRNA genes of strains X-1125T and X-1174 were highly similar (99.7 %) to each other.
30860460	2	52	theme	halotolerant	182:193	arg1	X-1174					226:231	X-1174	226:231	X-1174	226:231	Two rod-shaped, slightly halophilic and extremely halotolerant bacterial strains (X-1125T and X-1174), which were Gram-stain-positive, facultatively anaerobic and motile with peritrichous flagella, were isolated from the faeces of Tibetan antelopes.
30860460	2	52	theme	halotolerant	182:193	arg1	X-1125T					214:220	X-1125T	214:220	X-1125T	214:220	Two rod-shaped, slightly halophilic and extremely halotolerant bacterial strains (X-1125T and X-1174), which were Gram-stain-positive, facultatively anaerobic and motile with peritrichous flagella, were isolated from the faeces of Tibetan antelopes.
30860460	2	52	theme	halotolerant	182:193	arg1	strains					205:211	Two rod-shaped, slightly halophilic and extremely halotolerant bacterial strains	132:211	Two rod-shaped, slightly halophilic and extremely halotolerant bacterial strains (X-1125T and X-1174)	132:232	Two rod-shaped, slightly halophilic and extremely halotolerant bacterial strains (X-1125T and X-1174), which were Gram-stain-positive, facultatively anaerobic and motile with peritrichous flagella, were isolated from the faeces of Tibetan antelopes.
30860460	6	53	theme	sole	1022:1025	arg1	menaquinone					1027:1037	the sole menaquinone	1018:1037	the sole menaquinone (100 %)	1018:1045	MK-7 was the sole menaquinone (100 %).
30860460	6	53	theme	sole	1022:1025	arg1	%					1044:1044	100 %	1040:1044	100 %	1040:1044	MK-7 was the sole menaquinone (100 %).
30860460	6	53	theme	sole	1022:1025	arg1	MK-7					1009:1012	MK-7	1009:1012	MK-7	1009:1012	MK-7 was the sole menaquinone (100 %).
30860460	4	54	theme	phylogenetic	606:617	arg1	neighbours					619:628	their nearest phylogenetic neighbours	592:628	their nearest phylogenetic neighbours	592:628	Based on the results of 16S rRNA gene sequences, and phylogenetic and phylogenomic analyses, their nearest phylogenetic neighbours were Paraliobacillussediminis KCTC 33762T (98.4 % similarity), Paraliobacillusquinghaiensis CGMCC 1.6333T (96.9 %) and Paraliobacillusryukyuensis NBRC 100001T (95.9 %) while the 16S rRNA genes of strains X-1125T and X-1174 were highly similar (99.7 %) to each other.
30860460	7	55	theme	glutamic	1090:1097	arg1	acid					1099:1102	glutamic acid	1090:1102	glutamic acid	1090:1102	The cell wall contained alanine, glycine, glutamic acid and meso-diaminopimelic acid.
30860460	1	56	attach	isolated	93:100	arg2	bacterium					83:91	a slightly halophilic and extremely halotolerant bacterium	34:91	a slightly halophilic and extremely halotolerant bacterium isolated from Tibetan antelope faeces	34:129	nov., a slightly halophilic and extremely halotolerant bacterium isolated from Tibetan antelope faeces.
30860460	1	56	attach	isolated	93:100	arg1	faeces					124:129	Tibetan antelope faeces	107:129	Tibetan antelope faeces	107:129	nov., a slightly halophilic and extremely halotolerant bacterium isolated from Tibetan antelope faeces.
30860460	10	57	theme	mol	1488:1490	arg1	G+C contents					1474:1485	DNA G+C contents	1470:1485	DNA G+C contents (mol%) of 35.2 and 35.1 %	1470:1511	Strains X-1125T and X-1174 had DNA G+C contents (mol%) of 35.2 and 35.1 %, respectively.
30860460	10	57	theme	mol	1488:1490	arg1	%					1491:1491	mol%	1488:1491	mol%	1488:1491	Strains X-1125T and X-1174 had DNA G+C contents (mol%) of 35.2 and 35.1 %, respectively.
30860460	3	58	theme	3 	458:459	arg1	%					460:460	%	460:460	%	460:460	Their optimal temperature, NaCl concentration and pH for growth were 28 °C, 3 % (w/v) NaCl and pH 7.5, respectively.
30860460	3	59	theme	NaCl	409:412	arg1	concentration					414:426	NaCl concentration	409:426	NaCl concentration	409:426	Their optimal temperature, NaCl concentration and pH for growth were 28 °C, 3 % (w/v) NaCl and pH 7.5, respectively.
30860460	2	60	with	Gram-stain-positive	246:264	arg1	flagella					320:327	peritrichous flagella	307:327	peritrichous flagella	307:327	Two rod-shaped, slightly halophilic and extremely halotolerant bacterial strains (X-1125T and X-1174), which were Gram-stain-positive, facultatively anaerobic and motile with peritrichous flagella, were isolated from the faeces of Tibetan antelopes.
30860460	9	61	theme	DNA-DNA	1232:1238	arg1	hybridization					1240:1252	The in silico DNA-DNA hybridization	1218:1252	The in silico DNA-DNA hybridization value between strains X-1125T and X-1174	1218:1293	The in silico DNA-DNA hybridization value between strains X-1125T and X-1174 was 97.8 % (well above the species threshold), but their values were lower than the 70 % threshold with the three closely related type strains.
30860460	5	62	theme	unidentified	988:999	arg1	diphosphatidylglycerol					924:945	diphosphatidylglycerol	924:945	diphosphatidylglycerol	924:945	The polar lipids comprised diphosphatidylglycerol, two unidentified phospholipids and four unidentified lipids.
30860460	5	62	theme	unidentified	988:999	arg1	lipids					1001:1006	four unidentified lipids	983:1006	four unidentified lipids	983:1006	The polar lipids comprised diphosphatidylglycerol, two unidentified phospholipids and four unidentified lipids.
30860460	1	63	theme	antelope	115:122	arg1	faeces					124:129	Tibetan antelope faeces	107:129	Tibetan antelope faeces	107:129	nov., a slightly halophilic and extremely halotolerant bacterium isolated from Tibetan antelope faeces.
30860460	4	64	theme	phylogenetic	552:563	arg1	analyses					582:589	phylogenetic and phylogenomic analyses	552:589	phylogenetic and phylogenomic analyses	552:589	Based on the results of 16S rRNA gene sequences, and phylogenetic and phylogenomic analyses, their nearest phylogenetic neighbours were Paraliobacillussediminis KCTC 33762T (98.4 % similarity), Paraliobacillusquinghaiensis CGMCC 1.6333T (96.9 %) and Paraliobacillusryukyuensis NBRC 100001T (95.9 %) while the 16S rRNA genes of strains X-1125T and X-1174 were highly similar (99.7 %) to each other.
30860460	11	65	dep	strains	1557:1563	arg1	X-1174					1577:1582	X-1174	1577:1582	X-1174	1577:1582	Based on the presented data, strains X-1125T and X-1174 hereby represent a novel species of the genus Paraliobacillus, for which the name Paraliobacillus zengyii sp.
30860460	11	65	dep	strains	1557:1563	arg1	X-1125T					1565:1571	X-1125T	1565:1571	X-1125T	1565:1571	Based on the presented data, strains X-1125T and X-1174 hereby represent a novel species of the genus Paraliobacillus, for which the name Paraliobacillus zengyii sp.
30860460	11	65	dep	strains	1557:1563	arg1	strains					1557:1563	strains	1557:1563	strains X-1125T and X-1174	1557:1582	Based on the presented data, strains X-1125T and X-1174 hereby represent a novel species of the genus Paraliobacillus, for which the name Paraliobacillus zengyii sp.
30860460	10	66	theme	%	1511:1511	arg1	G+C contents					1474:1485	DNA G+C contents	1470:1485	DNA G+C contents (mol%) of 35.2 and 35.1 %	1470:1511	Strains X-1125T and X-1174 had DNA G+C contents (mol%) of 35.2 and 35.1 %, respectively.
30860460	10	66	theme	%	1511:1511	arg1	%					1491:1491	mol%	1488:1491	mol%	1488:1491	Strains X-1125T and X-1174 had DNA G+C contents (mol%) of 35.2 and 35.1 %, respectively.
30860460	8	67	dep	anteiso-C15 	1168:1179	arg1	anteiso-C15 					1168:1179	anteiso-C15 	1168:1179	anteiso-C15 	1168:1179	The major fatty acids (>9 %) were anteiso-C15 : 0, anteiso-C17 : 0 and C16 : 1ω11c.
30860460	8	67	dep	anteiso-C15 	1168:1179	arg1	anteiso-C17 					1185:1196	anteiso-C17 	1185:1196	anteiso-C17 	1185:1196	The major fatty acids (>9 %) were anteiso-C15 : 0, anteiso-C17 : 0 and C16 : 1ω11c.
30860460	8	67	dep	anteiso-C15 	1168:1179	arg1	acids					1150:1154	The major fatty acids	1134:1154	The major fatty acids (>9 %)	1134:1161	The major fatty acids (>9 %) were anteiso-C15 : 0, anteiso-C17 : 0 and C16 : 1ω11c.
30860460	8	67	dep	anteiso-C15 	1168:1179	arg1	 0					1181:1182	 0	1181:1182	 0	1181:1182	The major fatty acids (>9 %) were anteiso-C15 : 0, anteiso-C17 : 0 and C16 : 1ω11c.
30860460	9	68	dep	strains	1268:1274	arg1	strains					1268:1274	strains X-1125T and X-1174	1268:1293	strains X-1125T and X-1174	1268:1293	The in silico DNA-DNA hybridization value between strains X-1125T and X-1174 was 97.8 % (well above the species threshold), but their values were lower than the 70 % threshold with the three closely related type strains.
30860460	9	68	dep	strains	1268:1274	arg1	X-1174					1288:1293	X-1174	1288:1293	X-1174	1288:1293	The in silico DNA-DNA hybridization value between strains X-1125T and X-1174 was 97.8 % (well above the species threshold), but their values were lower than the 70 % threshold with the three closely related type strains.
30860460	9	68	dep	strains	1268:1274	arg1	X-1125T					1276:1282	X-1125T	1276:1282	X-1125T	1276:1282	The in silico DNA-DNA hybridization value between strains X-1125T and X-1174 was 97.8 % (well above the species threshold), but their values were lower than the 70 % threshold with the three closely related type strains.
30860460	4	69	theme	Paraliobacillusquinghaiensis	693:720	arg1	1.6333T					728:734	Paraliobacillusquinghaiensis CGMCC 1.6333T	693:734	Paraliobacillusquinghaiensis CGMCC 1.6333T (96.9 %)	693:743	Based on the results of 16S rRNA gene sequences, and phylogenetic and phylogenomic analyses, their nearest phylogenetic neighbours were Paraliobacillussediminis KCTC 33762T (98.4 % similarity), Paraliobacillusquinghaiensis CGMCC 1.6333T (96.9 %) and Paraliobacillusryukyuensis NBRC 100001T (95.9 %) while the 16S rRNA genes of strains X-1125T and X-1174 were highly similar (99.7 %) to each other.
30860460	4	69	theme	Paraliobacillusquinghaiensis	693:720	arg1	%					742:742	96.9 %	737:742	96.9 %	737:742	Based on the results of 16S rRNA gene sequences, and phylogenetic and phylogenomic analyses, their nearest phylogenetic neighbours were Paraliobacillussediminis KCTC 33762T (98.4 % similarity), Paraliobacillusquinghaiensis CGMCC 1.6333T (96.9 %) and Paraliobacillusryukyuensis NBRC 100001T (95.9 %) while the 16S rRNA genes of strains X-1125T and X-1174 were highly similar (99.7 %) to each other.
30860460	8	70	dep	 0	1181:1182	arg1	C16 					1205:1208	C16 	1205:1208	C16 	1205:1208	The major fatty acids (>9 %) were anteiso-C15 : 0, anteiso-C17 : 0 and C16 : 1ω11c.
30860460	8	70	dep	 0	1181:1182	arg1	 0					1198:1199	 0	1198:1199	 0	1198:1199	The major fatty acids (>9 %) were anteiso-C15 : 0, anteiso-C17 : 0 and C16 : 1ω11c.
30860460	8	70	dep	 0	1181:1182	arg1	 1ω11c					1210:1215	 1ω11c	1210:1215	 1ω11c	1210:1215	The major fatty acids (>9 %) were anteiso-C15 : 0, anteiso-C17 : 0 and C16 : 1ω11c.
30860460	11	71	theme	presented	1541:1549	arg1	data					1551:1554	the presented data	1537:1554	the presented data	1537:1554	Based on the presented data, strains X-1125T and X-1174 hereby represent a novel species of the genus Paraliobacillus, for which the name Paraliobacillus zengyii sp.
30860460	4	72	theme	NBRC	776:779	arg1	100001T					781:787	Paraliobacillusryukyuensis NBRC 100001T	749:787	Paraliobacillusryukyuensis NBRC 100001T (95.9 %)	749:796	Based on the results of 16S rRNA gene sequences, and phylogenetic and phylogenomic analyses, their nearest phylogenetic neighbours were Paraliobacillussediminis KCTC 33762T (98.4 % similarity), Paraliobacillusquinghaiensis CGMCC 1.6333T (96.9 %) and Paraliobacillusryukyuensis NBRC 100001T (95.9 %) while the 16S rRNA genes of strains X-1125T and X-1174 were highly similar (99.7 %) to each other.
30860460	4	72	theme	NBRC	776:779	arg1	%					795:795	95.9 %	790:795	95.9 %	790:795	Based on the results of 16S rRNA gene sequences, and phylogenetic and phylogenomic analyses, their nearest phylogenetic neighbours were Paraliobacillussediminis KCTC 33762T (98.4 % similarity), Paraliobacillusquinghaiensis CGMCC 1.6333T (96.9 %) and Paraliobacillusryukyuensis NBRC 100001T (95.9 %) while the 16S rRNA genes of strains X-1125T and X-1174 were highly similar (99.7 %) to each other.
30860460	9	73	theme	70 	1379:1381	arg1	%					1382:1382	%	1382:1382	%	1382:1382	The in silico DNA-DNA hybridization value between strains X-1125T and X-1174 was 97.8 % (well above the species threshold), but their values were lower than the 70 % threshold with the three closely related type strains.
30860460	2	74	dep	strains	205:211	arg1	X-1174					226:231	X-1174	226:231	X-1174	226:231	Two rod-shaped, slightly halophilic and extremely halotolerant bacterial strains (X-1125T and X-1174), which were Gram-stain-positive, facultatively anaerobic and motile with peritrichous flagella, were isolated from the faeces of Tibetan antelopes.
30860460	2	74	dep	strains	205:211	arg1	X-1125T					214:220	X-1125T	214:220	X-1125T	214:220	Two rod-shaped, slightly halophilic and extremely halotolerant bacterial strains (X-1125T and X-1174), which were Gram-stain-positive, facultatively anaerobic and motile with peritrichous flagella, were isolated from the faeces of Tibetan antelopes.
30860460	2	74	dep	strains	205:211	arg1	strains					205:211	Two rod-shaped, slightly halophilic and extremely halotolerant bacterial strains	132:211	Two rod-shaped, slightly halophilic and extremely halotolerant bacterial strains (X-1125T and X-1174)	132:232	Two rod-shaped, slightly halophilic and extremely halotolerant bacterial strains (X-1125T and X-1174), which were Gram-stain-positive, facultatively anaerobic and motile with peritrichous flagella, were isolated from the faeces of Tibetan antelopes.
30860460	4	75	theme	nearest	598:604	arg1	neighbours					619:628	their nearest phylogenetic neighbours	592:628	their nearest phylogenetic neighbours	592:628	Based on the results of 16S rRNA gene sequences, and phylogenetic and phylogenomic analyses, their nearest phylogenetic neighbours were Paraliobacillussediminis KCTC 33762T (98.4 % similarity), Paraliobacillusquinghaiensis CGMCC 1.6333T (96.9 %) and Paraliobacillusryukyuensis NBRC 100001T (95.9 %) while the 16S rRNA genes of strains X-1125T and X-1174 were highly similar (99.7 %) to each other.
30860460	3	76	dep	%	460:460	arg1	w/v					463:465	w/v	463:465	w/v	463:465	Their optimal temperature, NaCl concentration and pH for growth were 28 °C, 3 % (w/v) NaCl and pH 7.5, respectively.
30860460	9	77	theme	%	1382:1382	arg1	threshold					1384:1392	the 70 % threshold	1375:1392	the 70 % threshold with the three closely related type strains	1375:1436	The in silico DNA-DNA hybridization value between strains X-1125T and X-1174 was 97.8 % (well above the species threshold), but their values were lower than the 70 % threshold with the three closely related type strains.
30860460	11	78	theme	Paraliobacillus	1630:1644	arg1	species					1609:1615	a novel species	1601:1615	a novel species	1601:1615	Based on the presented data, strains X-1125T and X-1174 hereby represent a novel species of the genus Paraliobacillus, for which the name Paraliobacillus zengyii sp.
30860460	4	79	theme	analyses	582:589	arg1	results					512:518	the results	508:518	the results of 16S rRNA gene sequences, and phylogenetic and phylogenomic analyses	508:589	Based on the results of 16S rRNA gene sequences, and phylogenetic and phylogenomic analyses, their nearest phylogenetic neighbours were Paraliobacillussediminis KCTC 33762T (98.4 % similarity), Paraliobacillusquinghaiensis CGMCC 1.6333T (96.9 %) and Paraliobacillusryukyuensis NBRC 100001T (95.9 %) while the 16S rRNA genes of strains X-1125T and X-1174 were highly similar (99.7 %) to each other.
30860460	4	80	dep	similar	865:871	arg1	genes					817:821	the 16S rRNA genes	804:821	the 16S rRNA genes of strains X-1125T and X-1174	804:851	Based on the results of 16S rRNA gene sequences, and phylogenetic and phylogenomic analyses, their nearest phylogenetic neighbours were Paraliobacillussediminis KCTC 33762T (98.4 % similarity), Paraliobacillusquinghaiensis CGMCC 1.6333T (96.9 %) and Paraliobacillusryukyuensis NBRC 100001T (95.9 %) while the 16S rRNA genes of strains X-1125T and X-1174 were highly similar (99.7 %) to each other.
30860460	4	80	dep	similar	865:871	arg1	%					879:879	99.7 %	874:879	99.7 %	874:879	Based on the results of 16S rRNA gene sequences, and phylogenetic and phylogenomic analyses, their nearest phylogenetic neighbours were Paraliobacillussediminis KCTC 33762T (98.4 % similarity), Paraliobacillusquinghaiensis CGMCC 1.6333T (96.9 %) and Paraliobacillusryukyuensis NBRC 100001T (95.9 %) while the 16S rRNA genes of strains X-1125T and X-1174 were highly similar (99.7 %) to each other.
30860460	4	80	dep	similar	865:871	arg1	similar					865:871	similar	865:871	similar	865:871	Based on the results of 16S rRNA gene sequences, and phylogenetic and phylogenomic analyses, their nearest phylogenetic neighbours were Paraliobacillussediminis KCTC 33762T (98.4 % similarity), Paraliobacillusquinghaiensis CGMCC 1.6333T (96.9 %) and Paraliobacillusryukyuensis NBRC 100001T (95.9 %) while the 16S rRNA genes of strains X-1125T and X-1174 were highly similar (99.7 %) to each other.
30860460	8	81	theme	major	1138:1142	arg1	anteiso-C15 					1168:1179	anteiso-C15 	1168:1179	anteiso-C15 	1168:1179	The major fatty acids (>9 %) were anteiso-C15 : 0, anteiso-C17 : 0 and C16 : 1ω11c.
30860460	8	81	theme	major	1138:1142	arg1	 0					1181:1182	 0	1181:1182	 0	1181:1182	The major fatty acids (>9 %) were anteiso-C15 : 0, anteiso-C17 : 0 and C16 : 1ω11c.
30860460	8	81	theme	major	1138:1142	arg1	acids					1150:1154	The major fatty acids	1134:1154	The major fatty acids (>9 %)	1134:1161	The major fatty acids (>9 %) were anteiso-C15 : 0, anteiso-C17 : 0 and C16 : 1ω11c.
30860460	8	81	theme	major	1138:1142	arg1	%					1160:1160	>9 %	1157:1160	>9 %	1157:1160	The major fatty acids (>9 %) were anteiso-C15 : 0, anteiso-C17 : 0 and C16 : 1ω11c.
30860460	2	82	theme	antelopes	371:379	arg1	faeces					353:358	the faeces	349:358	the faeces of Tibetan antelopes	349:379	Two rod-shaped, slightly halophilic and extremely halotolerant bacterial strains (X-1125T and X-1174), which were Gram-stain-positive, facultatively anaerobic and motile with peritrichous flagella, were isolated from the faeces of Tibetan antelopes.
30860460	4	83	theme	phylogenomic	569:580	arg1	analyses					582:589	phylogenetic and phylogenomic analyses	552:589	phylogenetic and phylogenomic analyses	552:589	Based on the results of 16S rRNA gene sequences, and phylogenetic and phylogenomic analyses, their nearest phylogenetic neighbours were Paraliobacillussediminis KCTC 33762T (98.4 % similarity), Paraliobacillusquinghaiensis CGMCC 1.6333T (96.9 %) and Paraliobacillusryukyuensis NBRC 100001T (95.9 %) while the 16S rRNA genes of strains X-1125T and X-1174 were highly similar (99.7 %) to each other.
31459968	1	0	theme	food	164:167	arg1	applications					179:190	biomedical and food packaging applications	149:190	biomedical and food packaging applications	149:190	Composite films of proteins and polysaccharides have a broad range of biomedical and food packaging applications, in which they are frequently exposed to fluid environments with varying ionic strengths.
31459968	2	1	attach	derived	346:352	arg1	gelatin					374:380	gelatin	374:380	gelatin (GEL)	374:386	In the present work, we report the behavior of biopolymer films derived from chitosan (Ch), gelatin (GEL), and Ch/GEL mixture in salt solutions with varying concentrations and ion charges.
31459968	2	1	attach	derived	346:352	arg1	mixture					400:406	Ch/GEL mixture	393:406	Ch/GEL mixture	393:406	In the present work, we report the behavior of biopolymer films derived from chitosan (Ch), gelatin (GEL), and Ch/GEL mixture in salt solutions with varying concentrations and ion charges.
31459968	2	1	attach	derived	346:352	arg2	films					340:344	biopolymer films	329:344	biopolymer films derived from chitosan (Ch), gelatin (GEL), and Ch/GEL mixture	329:406	In the present work, we report the behavior of biopolymer films derived from chitosan (Ch), gelatin (GEL), and Ch/GEL mixture in salt solutions with varying concentrations and ion charges.
31459968	2	1	attach	derived	346:352	arg1	chitosan					359:366	chitosan	359:366	chitosan (Ch)	359:371	In the present work, we report the behavior of biopolymer films derived from chitosan (Ch), gelatin (GEL), and Ch/GEL mixture in salt solutions with varying concentrations and ion charges.
31459968	2	1	attach	derived	346:352	arg1	GEL					383:385	GEL	383:385	GEL	383:385	In the present work, we report the behavior of biopolymer films derived from chitosan (Ch), gelatin (GEL), and Ch/GEL mixture in salt solutions with varying concentrations and ion charges.
31459968	2	1	attach	derived	346:352	arg1	Ch					369:370	Ch	369:370	Ch	369:370	In the present work, we report the behavior of biopolymer films derived from chitosan (Ch), gelatin (GEL), and Ch/GEL mixture in salt solutions with varying concentrations and ion charges.
31459968	1	2	theme	packaging	169:177	arg1	applications					179:190	biomedical and food packaging applications	149:190	biomedical and food packaging applications	149:190	Composite films of proteins and polysaccharides have a broad range of biomedical and food packaging applications, in which they are frequently exposed to fluid environments with varying ionic strengths.
31459968	1	3	theme	ionic	265:269	arg1	strengths					271:279	ionic strengths	265:279	ionic strengths	265:279	Composite films of proteins and polysaccharides have a broad range of biomedical and food packaging applications, in which they are frequently exposed to fluid environments with varying ionic strengths.
31459968	6	4	theme	varying	1060:1066	arg1	environments					1074:1085	varying ionic environments	1060:1085	varying ionic environments	1060:1085	These results provide insight into the behavior of protein/polysaccharide films in varying ionic environments, thus enabling enhanced design of biomaterials for a broad range of applications.
31459968	3	5	theme	polyampholyte	647:659	arg1	behavior					661:668	a polyampholyte behavior	645:668	a polyampholyte behavior	645:668	The swelling and dissolution of the Ch films reduced with increasing salt concentration due to the polyelectrolyte behavior of this biopolymer, while the GEL films displayed a polyampholyte behavior, in which film swelling and dissolution were enhanced in salt solutions.
31459968	2	6	from	behavior	317:324	arg1	solutions					416:424	salt solutions	411:424	salt solutions with varying concentrations and ion charges	411:468	In the present work, we report the behavior of biopolymer films derived from chitosan (Ch), gelatin (GEL), and Ch/GEL mixture in salt solutions with varying concentrations and ion charges.
31459968	5	7	theme	screened	909:916	arg1	attraction					918:927	the screened attraction	905:927	the screened attraction between these molecules and the polymer matrix	905:974	The release of small ionic and zwitter-ionic molecules from the films was enhanced in ionic solutions due to the screened attraction between these molecules and the polymer matrix.
31459968	3	8	theme	Ch	507:508	arg1	films					510:514	the Ch films	503:514	the Ch films	503:514	The swelling and dissolution of the Ch films reduced with increasing salt concentration due to the polyelectrolyte behavior of this biopolymer, while the GEL films displayed a polyampholyte behavior, in which film swelling and dissolution were enhanced in salt solutions.
31459968	3	9	theme	film	680:683	arg1	swelling					685:692	film swelling	680:692	film swelling	680:692	The swelling and dissolution of the Ch films reduced with increasing salt concentration due to the polyelectrolyte behavior of this biopolymer, while the GEL films displayed a polyampholyte behavior, in which film swelling and dissolution were enhanced in salt solutions.
31459968	2	10	theme	present	289:295	arg1	work					297:300	the present work	285:300	the present work	285:300	In the present work, we report the behavior of biopolymer films derived from chitosan (Ch), gelatin (GEL), and Ch/GEL mixture in salt solutions with varying concentrations and ion charges.
31459968	6	11	from	films	1051:1055	arg1	environments					1074:1085	varying ionic environments	1060:1085	varying ionic environments	1060:1085	These results provide insight into the behavior of protein/polysaccharide films in varying ionic environments, thus enabling enhanced design of biomaterials for a broad range of applications.
31459968	1	12	theme	Composite	79:87	arg1	films					89:93	Composite films	79:93	Composite films of proteins and polysaccharides	79:125	Composite films of proteins and polysaccharides have a broad range of biomedical and food packaging applications, in which they are frequently exposed to fluid environments with varying ionic strengths.
31459968	5	13	theme	ionic	882:886	arg1	solutions					888:896	ionic solutions	882:896	ionic solutions due to the screened attraction between these molecules and the polymer matrix	882:974	The release of small ionic and zwitter-ionic molecules from the films was enhanced in ionic solutions due to the screened attraction between these molecules and the polymer matrix.
31459968	6	14	theme	enhanced	1102:1109	arg1	design					1111:1116	enhanced design	1102:1116	enhanced design of biomaterials	1102:1132	These results provide insight into the behavior of protein/polysaccharide films in varying ionic environments, thus enabling enhanced design of biomaterials for a broad range of applications.
31459968	6	15	theme	films	1051:1055	arg1	behavior					1016:1023	the behavior	1012:1023	the behavior of protein/polysaccharide films in varying ionic environments	1012:1085	These results provide insight into the behavior of protein/polysaccharide films in varying ionic environments, thus enabling enhanced design of biomaterials for a broad range of applications.
31459968	6	16	theme	broad	1140:1144	arg1	range					1146:1150	a broad range	1138:1150	a broad range of applications	1138:1166	These results provide insight into the behavior of protein/polysaccharide films in varying ionic environments, thus enabling enhanced design of biomaterials for a broad range of applications.
31459968	1	17	theme	applications	179:190	arg1	range					140:144	a broad range	132:144	a broad range of biomedical and food packaging applications, in which they are frequently exposed to fluid environments with varying ionic strengths	132:279	Composite films of proteins and polysaccharides have a broad range of biomedical and food packaging applications, in which they are frequently exposed to fluid environments with varying ionic strengths.
31459968	0	18	theme	Chitosan/Gelatin	55:70	arg1	Films					72:76	Composite Chitosan/Gelatin Films	45:76	Composite Chitosan/Gelatin Films	45:76	Polyelectrolyte vs Polyampholyte Behavior of Composite Chitosan/Gelatin Films.
31459968	0	19	theme	Composite	45:53	arg1	Films					72:76	Composite Chitosan/Gelatin Films	45:76	Composite Chitosan/Gelatin Films	45:76	Polyelectrolyte vs Polyampholyte Behavior of Composite Chitosan/Gelatin Films.
31459968	5	20	theme	ionic	817:821	arg1	molecules					841:849	small ionic and zwitter-ionic molecules	811:849	small ionic and zwitter-ionic molecules	811:849	The release of small ionic and zwitter-ionic molecules from the films was enhanced in ionic solutions due to the screened attraction between these molecules and the polymer matrix.
31459968	1	21	theme	proteins	98:105	arg1	films					89:93	Composite films	79:93	Composite films of proteins and polysaccharides	79:125	Composite films of proteins and polysaccharides have a broad range of biomedical and food packaging applications, in which they are frequently exposed to fluid environments with varying ionic strengths.
31459968	5	22	theme	due	898:900	arg1	solutions					888:896	ionic solutions	882:896	ionic solutions due to the screened attraction between these molecules and the polymer matrix	882:974	The release of small ionic and zwitter-ionic molecules from the films was enhanced in ionic solutions due to the screened attraction between these molecules and the polymer matrix.
31459968	5	23	theme	zwitter-ionic	827:839	arg1	molecules					841:849	small ionic and zwitter-ionic molecules	811:849	small ionic and zwitter-ionic molecules	811:849	The release of small ionic and zwitter-ionic molecules from the films was enhanced in ionic solutions due to the screened attraction between these molecules and the polymer matrix.
31459968	2	24	theme	Ch/GEL	393:398	arg1	mixture					400:406	Ch/GEL mixture	393:406	Ch/GEL mixture	393:406	In the present work, we report the behavior of biopolymer films derived from chitosan (Ch), gelatin (GEL), and Ch/GEL mixture in salt solutions with varying concentrations and ion charges.
31459968	1	25	theme	polysaccharides	111:125	arg1	films					89:93	Composite films	79:93	Composite films of proteins and polysaccharides	79:125	Composite films of proteins and polysaccharides have a broad range of biomedical and food packaging applications, in which they are frequently exposed to fluid environments with varying ionic strengths.
31459968	0	26	theme	Polyampholyte	19:31	arg1	Behavior					33:40	Polyampholyte Behavior	19:40	Polyampholyte Behavior	19:40	Polyelectrolyte vs Polyampholyte Behavior of Composite Chitosan/Gelatin Films.
31459968	5	27	theme	molecules	841:849	arg1	release					800:806	The release	796:806	The release of small ionic and zwitter-ionic molecules from the films	796:864	The release of small ionic and zwitter-ionic molecules from the films was enhanced in ionic solutions due to the screened attraction between these molecules and the polymer matrix.
31459968	2	28	theme	varying	431:437	arg1	concentrations					439:452	varying concentrations	431:452	varying concentrations	431:452	In the present work, we report the behavior of biopolymer films derived from chitosan (Ch), gelatin (GEL), and Ch/GEL mixture in salt solutions with varying concentrations and ion charges.
31459968	2	29	theme	biopolymer	329:338	arg1	films					340:344	biopolymer films	329:344	biopolymer films derived from chitosan (Ch), gelatin (GEL), and Ch/GEL mixture	329:406	In the present work, we report the behavior of biopolymer films derived from chitosan (Ch), gelatin (GEL), and Ch/GEL mixture in salt solutions with varying concentrations and ion charges.
31459968	5	30	theme	polymer	961:967	arg1	matrix					969:974	the polymer matrix	957:974	the polymer matrix	957:974	The release of small ionic and zwitter-ionic molecules from the films was enhanced in ionic solutions due to the screened attraction between these molecules and the polymer matrix.
31459968	6	31	theme	applications	1155:1166	arg1	range					1146:1150	a broad range	1138:1150	a broad range of applications	1138:1166	These results provide insight into the behavior of protein/polysaccharide films in varying ionic environments, thus enabling enhanced design of biomaterials for a broad range of applications.
31459968	5	32	theme	small	811:815	arg1	molecules					841:849	small ionic and zwitter-ionic molecules	811:849	small ionic and zwitter-ionic molecules	811:849	The release of small ionic and zwitter-ionic molecules from the films was enhanced in ionic solutions due to the screened attraction between these molecules and the polymer matrix.
31459968	1	33	contain	have	127:130	arg1	films					89:93	Composite films	79:93	Composite films of proteins and polysaccharides	79:125	Composite films of proteins and polysaccharides have a broad range of biomedical and food packaging applications, in which they are frequently exposed to fluid environments with varying ionic strengths.
31459968	1	33	contain	have	127:130	arg2	range					140:144	a broad range	132:144	a broad range of biomedical and food packaging applications, in which they are frequently exposed to fluid environments with varying ionic strengths	132:279	Composite films of proteins and polysaccharides have a broad range of biomedical and food packaging applications, in which they are frequently exposed to fluid environments with varying ionic strengths.
31459968	0	34	theme	Films	72:76	arg1	Polyelectrolyte					0:14	Polyelectrolyte	0:14	Polyelectrolyte	0:14	Polyelectrolyte vs Polyampholyte Behavior of Composite Chitosan/Gelatin Films.
31459968	3	35	theme	biopolymer	603:612	arg1	behavior					586:593	the polyelectrolyte behavior	566:593	the polyelectrolyte behavior of this biopolymer	566:612	The swelling and dissolution of the Ch films reduced with increasing salt concentration due to the polyelectrolyte behavior of this biopolymer, while the GEL films displayed a polyampholyte behavior, in which film swelling and dissolution were enhanced in salt solutions.
31459968	3	36	theme	salt	540:543	arg1	concentration					545:557	salt concentration	540:557	salt concentration	540:557	The swelling and dissolution of the Ch films reduced with increasing salt concentration due to the polyelectrolyte behavior of this biopolymer, while the GEL films displayed a polyampholyte behavior, in which film swelling and dissolution were enhanced in salt solutions.
31459968	2	37	theme	films	340:344	arg1	behavior					317:324	the behavior	313:324	the behavior of biopolymer films derived from chitosan (Ch), gelatin (GEL), and Ch/GEL mixture in salt solutions with varying concentrations and ion charges	313:468	In the present work, we report the behavior of biopolymer films derived from chitosan (Ch), gelatin (GEL), and Ch/GEL mixture in salt solutions with varying concentrations and ion charges.
31459968	2	38	theme	salt	411:414	arg1	solutions					416:424	salt solutions	411:424	salt solutions with varying concentrations and ion charges	411:468	In the present work, we report the behavior of biopolymer films derived from chitosan (Ch), gelatin (GEL), and Ch/GEL mixture in salt solutions with varying concentrations and ion charges.
31459968	1	39	theme	broad	134:138	arg1	range					140:144	a broad range	132:144	a broad range of biomedical and food packaging applications, in which they are frequently exposed to fluid environments with varying ionic strengths	132:279	Composite films of proteins and polysaccharides have a broad range of biomedical and food packaging applications, in which they are frequently exposed to fluid environments with varying ionic strengths.
31459968	3	40	theme	salt	727:730	arg1	solutions					732:740	salt solutions	727:740	salt solutions	727:740	The swelling and dissolution of the Ch films reduced with increasing salt concentration due to the polyelectrolyte behavior of this biopolymer, while the GEL films displayed a polyampholyte behavior, in which film swelling and dissolution were enhanced in salt solutions.
31459968	4	41	theme	Ch/GEL	753:758	arg1	films					760:764	Composite Ch/GEL films	743:764	Composite Ch/GEL films	743:764	Composite Ch/GEL films followed the behavior of GEL.
31459968	5	42	from	films	860:864	arg1	release					800:806	The release	796:806	The release of small ionic and zwitter-ionic molecules from the films	796:864	The release of small ionic and zwitter-ionic molecules from the films was enhanced in ionic solutions due to the screened attraction between these molecules and the polymer matrix.
31459968	3	43	theme	films	510:514	arg1	dissolution					488:498	dissolution	488:498	dissolution	488:498	The swelling and dissolution of the Ch films reduced with increasing salt concentration due to the polyelectrolyte behavior of this biopolymer, while the GEL films displayed a polyampholyte behavior, in which film swelling and dissolution were enhanced in salt solutions.
31459968	3	43	theme	films	510:514	arg1	swelling					475:482	swelling	475:482	swelling	475:482	The swelling and dissolution of the Ch films reduced with increasing salt concentration due to the polyelectrolyte behavior of this biopolymer, while the GEL films displayed a polyampholyte behavior, in which film swelling and dissolution were enhanced in salt solutions.
31459968	4	44	theme	Composite	743:751	arg1	films					760:764	Composite Ch/GEL films	743:764	Composite Ch/GEL films	743:764	Composite Ch/GEL films followed the behavior of GEL.
31459968	2	45	with	solutions	416:424	arg1	concentrations					439:452	varying concentrations	431:452	varying concentrations	431:452	In the present work, we report the behavior of biopolymer films derived from chitosan (Ch), gelatin (GEL), and Ch/GEL mixture in salt solutions with varying concentrations and ion charges.
31459968	2	45	with	solutions	416:424	arg1	charges					462:468	ion charges	458:468	ion charges	458:468	In the present work, we report the behavior of biopolymer films derived from chitosan (Ch), gelatin (GEL), and Ch/GEL mixture in salt solutions with varying concentrations and ion charges.
31459968	3	46	dep	swelling	475:482	arg1	The					471:473	The	471:473	The	471:473	The swelling and dissolution of the Ch films reduced with increasing salt concentration due to the polyelectrolyte behavior of this biopolymer, while the GEL films displayed a polyampholyte behavior, in which film swelling and dissolution were enhanced in salt solutions.
31459968	6	47	from	behavior	1016:1023	arg1	environments					1074:1085	varying ionic environments	1060:1085	varying ionic environments	1060:1085	These results provide insight into the behavior of protein/polysaccharide films in varying ionic environments, thus enabling enhanced design of biomaterials for a broad range of applications.
31459968	1	48	theme	fluid	233:237	arg1	environments					239:250	fluid environments	233:250	fluid environments	233:250	Composite films of proteins and polysaccharides have a broad range of biomedical and food packaging applications, in which they are frequently exposed to fluid environments with varying ionic strengths.
31459968	3	49	theme	GEL	625:627	arg1	films					629:633	the GEL films	621:633	the GEL films	621:633	The swelling and dissolution of the Ch films reduced with increasing salt concentration due to the polyelectrolyte behavior of this biopolymer, while the GEL films displayed a polyampholyte behavior, in which film swelling and dissolution were enhanced in salt solutions.
31459968	4	50	theme	GEL	791:793	arg1	behavior					779:786	the behavior	775:786	the behavior of GEL	775:793	Composite Ch/GEL films followed the behavior of GEL.
31459968	1	51	theme	biomedical	149:158	arg1	applications					179:190	biomedical and food packaging applications	149:190	biomedical and food packaging applications	149:190	Composite films of proteins and polysaccharides have a broad range of biomedical and food packaging applications, in which they are frequently exposed to fluid environments with varying ionic strengths.
31459968	6	52	theme	protein/polysaccharide	1028:1049	arg1	films					1051:1055	protein/polysaccharide films	1028:1055	protein/polysaccharide films in varying ionic environments	1028:1085	These results provide insight into the behavior of protein/polysaccharide films in varying ionic environments, thus enabling enhanced design of biomaterials for a broad range of applications.
31459968	6	53	theme	biomaterials	1121:1132	arg1	design					1111:1116	enhanced design	1102:1116	enhanced design of biomaterials	1102:1132	These results provide insight into the behavior of protein/polysaccharide films in varying ionic environments, thus enabling enhanced design of biomaterials for a broad range of applications.
31459968	2	54	theme	ion	458:460	arg1	charges					462:468	ion charges	458:468	ion charges	458:468	In the present work, we report the behavior of biopolymer films derived from chitosan (Ch), gelatin (GEL), and Ch/GEL mixture in salt solutions with varying concentrations and ion charges.
31459968	3	55	theme	polyelectrolyte	570:584	arg1	behavior					586:593	the polyelectrolyte behavior	566:593	the polyelectrolyte behavior of this biopolymer	566:612	The swelling and dissolution of the Ch films reduced with increasing salt concentration due to the polyelectrolyte behavior of this biopolymer, while the GEL films displayed a polyampholyte behavior, in which film swelling and dissolution were enhanced in salt solutions.
31459968	6	56	from	environments	1074:1085	arg1	behavior					1016:1023	the behavior	1012:1023	the behavior of protein/polysaccharide films in varying ionic environments	1012:1085	These results provide insight into the behavior of protein/polysaccharide films in varying ionic environments, thus enabling enhanced design of biomaterials for a broad range of applications.
31459968	6	57	theme	ionic	1068:1072	arg1	environments					1074:1085	varying ionic environments	1060:1085	varying ionic environments	1060:1085	These results provide insight into the behavior of protein/polysaccharide films in varying ionic environments, thus enabling enhanced design of biomaterials for a broad range of applications.
31336215	8	0	theme	oxidative	1289:1297	arg1	stress					1299:1304	oxidative stress	1289:1304	oxidative stress	1289:1304	Urinary metabolomics revealed a significant increase in metabolites related to oxidative stress and metabolic inflammation together with an altered excretion of host-microbiota co-metabolites only in the CAF group.
31336215	1	1	theme	key	143:145	arg1	agent					159:163	a key influencing agent	141:163	a key influencing agent affecting the gut microbiome	141:192	Diet is considered a key influencing agent affecting the gut microbiome.
31336215	10	2	theme	urinary	1843:1849	arg1	profiles					1862:1869	urinary metabolome profiles	1843:1869	urinary metabolome profiles	1843:1869	Overall, our study suggests that different hypercaloric dietary models distinctively influence gut microbiota composition and reveals robust and similar clustering patterns concerning both cecal microbiome and urinary metabolome profiles.
31336215	7	3	from	Changes	1000:1006	arg1	activity					1021:1028	microbial activity	1011:1028	microbial activity according to dietary treatment	1011:1059	Changes in microbial activity according to dietary treatment were also reflected in the disruption of short-chain fatty acid production and bile acid metabolism, which were mainly associated with fiber intake.
31336215	4	4	dep	standard	545:552	arg1	LF					581:582	LF	581:582	LF	581:582	For this purpose, Wistar rats were fed standard, cafeteria (CAF), low-fat (LF), high-fat (HF) and high-fat high-sucrose (HFS) diets for 10 weeks.
31336215	4	4	dep	standard	545:552	arg1	CAF					566:568	CAF	566:568	CAF	566:568	For this purpose, Wistar rats were fed standard, cafeteria (CAF), low-fat (LF), high-fat (HF) and high-fat high-sucrose (HFS) diets for 10 weeks.
31336215	4	4	dep	standard	545:552	arg1	high-sucrose					613:624	high-fat high-sucrose	604:624	high-fat high-sucrose (HFS)	604:630	For this purpose, Wistar rats were fed standard, cafeteria (CAF), low-fat (LF), high-fat (HF) and high-fat high-sucrose (HFS) diets for 10 weeks.
31336215	4	4	dep	standard	545:552	arg1	high-fat					586:593	high-fat	586:593	high-fat (HF)	586:598	For this purpose, Wistar rats were fed standard, cafeteria (CAF), low-fat (LF), high-fat (HF) and high-fat high-sucrose (HFS) diets for 10 weeks.
31336215	4	4	dep	standard	545:552	arg1	HF					596:597	HF	596:597	HF	596:597	For this purpose, Wistar rats were fed standard, cafeteria (CAF), low-fat (LF), high-fat (HF) and high-fat high-sucrose (HFS) diets for 10 weeks.
31336215	4	4	dep	standard	545:552	arg1	HFS					627:629	HFS	627:629	HFS	627:629	For this purpose, Wistar rats were fed standard, cafeteria (CAF), low-fat (LF), high-fat (HF) and high-fat high-sucrose (HFS) diets for 10 weeks.
31336215	4	4	dep	standard	545:552	arg1	low-fat					572:578	low-fat	572:578	low-fat (LF)	572:583	For this purpose, Wistar rats were fed standard, cafeteria (CAF), low-fat (LF), high-fat (HF) and high-fat high-sucrose (HFS) diets for 10 weeks.
31336215	4	4	dep	standard	545:552	arg1	cafeteria					555:563	cafeteria	555:563	cafeteria (CAF)	555:569	For this purpose, Wistar rats were fed standard, cafeteria (CAF), low-fat (LF), high-fat (HF) and high-fat high-sucrose (HFS) diets for 10 weeks.
31336215	0	5	dep	approach	112:119	arg1	Impact					0:5	Impact	0:5	Impact of different hypercaloric diets on obesity features in rats	0:65	Impact of different hypercaloric diets on obesity features in rats: a metagenomics and metabolomics integrative approach.
31336215	9	6	theme	metabolic	1464:1472	arg1	patterns					1474:1481	metabolic patterns	1464:1481	metabolic patterns	1464:1481	Moreover, several associations between metabolic patterns, physiological status and specific microbial communities were described, helping to elucidate the crucial role of the microbiota in host homeostasis.
31336215	3	7	theme	metabolic	358:366	arg1	changes					368:374	the physiological, microbial and metabolic changes	325:374	the physiological, microbial and metabolic changes induced by different obesogenic diets	325:412	We aimed to characterize the physiological, microbial and metabolic changes induced by different obesogenic diets to understand the diet-specific modulation of the host-microbiota co-metabolism in rodents.
31336215	0	8	from	Impact	0:5	arg1	features					50:57	obesity features	42:57	obesity features in rats	42:65	Impact of different hypercaloric diets on obesity features in rats: a metagenomics and metabolomics integrative approach.
31336215	10	9	theme	microbiota	1732:1741	arg1	composition					1743:1753	gut microbiota composition	1728:1753	gut microbiota composition	1728:1753	Overall, our study suggests that different hypercaloric dietary models distinctively influence gut microbiota composition and reveals robust and similar clustering patterns concerning both cecal microbiome and urinary metabolome profiles.
31336215	8	10	from	increase	1254:1261	arg1	metabolites					1266:1276	metabolites	1266:1276	metabolites related to oxidative stress and metabolic inflammation	1266:1331	Urinary metabolomics revealed a significant increase in metabolites related to oxidative stress and metabolic inflammation together with an altered excretion of host-microbiota co-metabolites only in the CAF group.
31336215	0	11	dep	integrative	100:110	arg1	metagenomics					70:81	metagenomics	70:81	metagenomics	70:81	Impact of different hypercaloric diets on obesity features in rats: a metagenomics and metabolomics integrative approach.
31336215	8	12	theme	altered	1350:1356	arg1	excretion					1358:1366	an altered excretion	1347:1366	an altered excretion of host-microbiota co-metabolites	1347:1400	Urinary metabolomics revealed a significant increase in metabolites related to oxidative stress and metabolic inflammation together with an altered excretion of host-microbiota co-metabolites only in the CAF group.
31336215	6	13	theme	microbial	946:954	arg1	genera					956:961	few microbial genera	942:961	few microbial genera	942:961	Concerning the microbiome, CAF feeding induced a rise of the Bacteroidetes-to-Firmicutes ratio, while few microbial genera were altered in the HF or HFS group.
31336215	7	14	theme	acid	1145:1148	arg1	metabolism					1150:1159	bile acid metabolism	1140:1159	bile acid metabolism	1140:1159	Changes in microbial activity according to dietary treatment were also reflected in the disruption of short-chain fatty acid production and bile acid metabolism, which were mainly associated with fiber intake.
31336215	0	15	from	features	50:57	arg1	rats					62:65	rats	62:65	rats	62:65	Impact of different hypercaloric diets on obesity features in rats: a metagenomics and metabolomics integrative approach.
31336215	7	16	theme	fiber	1196:1200	arg1	intake					1202:1207	fiber intake	1196:1207	fiber intake	1196:1207	Changes in microbial activity according to dietary treatment were also reflected in the disruption of short-chain fatty acid production and bile acid metabolism, which were mainly associated with fiber intake.
31336215	10	17	theme	similar	1778:1784	arg1	patterns					1797:1804	robust and similar clustering patterns	1767:1804	robust and similar clustering patterns concerning both cecal microbiome and urinary metabolome profiles	1767:1869	Overall, our study suggests that different hypercaloric dietary models distinctively influence gut microbiota composition and reveals robust and similar clustering patterns concerning both cecal microbiome and urinary metabolome profiles.
31336215	6	18	theme	few	942:944	arg1	genera					956:961	few microbial genera	942:961	few microbial genera	942:961	Concerning the microbiome, CAF feeding induced a rise of the Bacteroidetes-to-Firmicutes ratio, while few microbial genera were altered in the HF or HFS group.
31336215	3	19	theme	microbial	344:352	arg1	changes					368:374	the physiological, microbial and metabolic changes	325:374	the physiological, microbial and metabolic changes induced by different obesogenic diets	325:412	We aimed to characterize the physiological, microbial and metabolic changes induced by different obesogenic diets to understand the diet-specific modulation of the host-microbiota co-metabolism in rodents.
31336215	7	20	theme	dietary	1043:1049	arg1	treatment					1051:1059	dietary treatment	1043:1059	dietary treatment	1043:1059	Changes in microbial activity according to dietary treatment were also reflected in the disruption of short-chain fatty acid production and bile acid metabolism, which were mainly associated with fiber intake.
31336215	10	21	theme	hypercaloric	1676:1687	arg1	models					1697:1702	different hypercaloric dietary models	1666:1702	different hypercaloric dietary models	1666:1702	Overall, our study suggests that different hypercaloric dietary models distinctively influence gut microbiota composition and reveals robust and similar clustering patterns concerning both cecal microbiome and urinary metabolome profiles.
31336215	5	22	theme	HFS	809:811	arg1	diets					813:817	both HF and HFS diets	797:817	both HF and HFS diets	797:817	The CAF diet strongly induced an obese phenotype accompanied by dyslipidemia, hyperleptinemia, insulin resistance and hepatic steatosis, whereas both HF and HFS diets promoted overweight.
31336215	0	23	theme	metabolomics	87:98	arg1	approach					112:119	a metagenomics and metabolomics integrative approach	68:119	a metagenomics and metabolomics integrative approach	68:119	Impact of different hypercaloric diets on obesity features in rats: a metagenomics and metabolomics integrative approach.
31336215	10	24	theme	different	1666:1674	arg1	models					1697:1702	different hypercaloric dietary models	1666:1702	different hypercaloric dietary models	1666:1702	Overall, our study suggests that different hypercaloric dietary models distinctively influence gut microbiota composition and reveals robust and similar clustering patterns concerning both cecal microbiome and urinary metabolome profiles.
31336215	2	25	theme	diseases	274:281	arg1	development					249:259	the development	245:259	the development of metabolic diseases such as obesity	245:297	Dysbiosis of microbial communities contributes to the development of metabolic diseases such as obesity.
31336215	0	26	theme	integrative	100:110	arg1	approach					112:119	a metagenomics and metabolomics integrative approach	68:119	a metagenomics and metabolomics integrative approach	68:119	Impact of different hypercaloric diets on obesity features in rats: a metagenomics and metabolomics integrative approach.
31336215	3	27	theme	different	387:395	arg1	diets					408:412	different obesogenic diets	387:412	different obesogenic diets	387:412	We aimed to characterize the physiological, microbial and metabolic changes induced by different obesogenic diets to understand the diet-specific modulation of the host-microbiota co-metabolism in rodents.
31336215	10	28	theme	cecal	1822:1826	arg1	microbiome					1828:1837	cecal microbiome	1822:1837	cecal microbiome	1822:1837	Overall, our study suggests that different hypercaloric dietary models distinctively influence gut microbiota composition and reveals robust and similar clustering patterns concerning both cecal microbiome and urinary metabolome profiles.
31336215	7	29	theme	production	1125:1134	arg1	disruption					1088:1097	the disruption	1084:1097	the disruption of short-chain fatty acid production and bile acid metabolism, which were mainly associated with fiber intake	1084:1207	Changes in microbial activity according to dietary treatment were also reflected in the disruption of short-chain fatty acid production and bile acid metabolism, which were mainly associated with fiber intake.
31336215	3	30	theme	diet-specific	432:444	arg1	modulation					446:455	the diet-specific modulation	428:455	the diet-specific modulation of the host-microbiota co-metabolism in rodents	428:503	We aimed to characterize the physiological, microbial and metabolic changes induced by different obesogenic diets to understand the diet-specific modulation of the host-microbiota co-metabolism in rodents.
31336215	3	31	theme	obesogenic	397:406	arg1	diets					408:412	different obesogenic diets	387:412	different obesogenic diets	387:412	We aimed to characterize the physiological, microbial and metabolic changes induced by different obesogenic diets to understand the diet-specific modulation of the host-microbiota co-metabolism in rodents.
31336215	1	32	theme	gut	179:181	arg1	microbiome					183:192	the gut microbiome	175:192	the gut microbiome	175:192	Diet is considered a key influencing agent affecting the gut microbiome.
31336215	0	33	theme	hypercaloric	20:31	arg1	diets					33:37	different hypercaloric diets	10:37	different hypercaloric diets	10:37	Impact of different hypercaloric diets on obesity features in rats: a metagenomics and metabolomics integrative approach.
31336215	5	34	theme	hepatic	770:776	arg1	steatosis					778:786	hepatic steatosis	770:786	hepatic steatosis	770:786	The CAF diet strongly induced an obese phenotype accompanied by dyslipidemia, hyperleptinemia, insulin resistance and hepatic steatosis, whereas both HF and HFS diets promoted overweight.
31336215	7	35	theme	microbial	1011:1019	arg1	activity					1021:1028	microbial activity	1011:1028	microbial activity according to dietary treatment	1011:1059	Changes in microbial activity according to dietary treatment were also reflected in the disruption of short-chain fatty acid production and bile acid metabolism, which were mainly associated with fiber intake.
31336215	8	36	theme	co-metabolites	1387:1400	arg1	excretion					1358:1366	an altered excretion	1347:1366	an altered excretion of host-microbiota co-metabolites	1347:1400	Urinary metabolomics revealed a significant increase in metabolites related to oxidative stress and metabolic inflammation together with an altered excretion of host-microbiota co-metabolites only in the CAF group.
31336215	6	37	theme	HFS	989:991	arg1	group					993:997	the HF or HFS group	979:997	group	993:997	Concerning the microbiome, CAF feeding induced a rise of the Bacteroidetes-to-Firmicutes ratio, while few microbial genera were altered in the HF or HFS group.
31336215	10	38	theme	metabolome	1851:1860	arg1	profiles					1862:1869	urinary metabolome profiles	1843:1869	urinary metabolome profiles	1843:1869	Overall, our study suggests that different hypercaloric dietary models distinctively influence gut microbiota composition and reveals robust and similar clustering patterns concerning both cecal microbiome and urinary metabolome profiles.
31336215	10	39	theme	dietary	1689:1695	arg1	models					1697:1702	different hypercaloric dietary models	1666:1702	different hypercaloric dietary models	1666:1702	Overall, our study suggests that different hypercaloric dietary models distinctively influence gut microbiota composition and reveals robust and similar clustering patterns concerning both cecal microbiome and urinary metabolome profiles.
31336215	0	40	theme	different	10:18	arg1	diets					33:37	different hypercaloric diets	10:37	different hypercaloric diets	10:37	Impact of different hypercaloric diets on obesity features in rats: a metagenomics and metabolomics integrative approach.
31336215	6	41	theme	ratio	929:933	arg1	rise					889:892	a rise	887:892	a rise of the Bacteroidetes-to-Firmicutes ratio	887:933	Concerning the microbiome, CAF feeding induced a rise of the Bacteroidetes-to-Firmicutes ratio, while few microbial genera were altered in the HF or HFS group.
31336215	5	42	theme	CAF	656:658	arg1	diet					660:663	The CAF diet	652:663	The CAF diet	652:663	The CAF diet strongly induced an obese phenotype accompanied by dyslipidemia, hyperleptinemia, insulin resistance and hepatic steatosis, whereas both HF and HFS diets promoted overweight.
31336215	1	43	theme	influencing	147:157	arg1	agent					159:163	a key influencing agent	141:163	a key influencing agent affecting the gut microbiome	141:192	Diet is considered a key influencing agent affecting the gut microbiome.
31336215	8	44	theme	Urinary	1210:1216	arg1	metabolomics					1218:1229	Urinary metabolomics	1210:1229	Urinary metabolomics	1210:1229	Urinary metabolomics revealed a significant increase in metabolites related to oxidative stress and metabolic inflammation together with an altered excretion of host-microbiota co-metabolites only in the CAF group.
31336215	6	45	theme	Bacteroidetes-to-Firmicutes	901:927	arg1	ratio					929:933	the Bacteroidetes-to-Firmicutes ratio	897:933	the Bacteroidetes-to-Firmicutes ratio	897:933	Concerning the microbiome, CAF feeding induced a rise of the Bacteroidetes-to-Firmicutes ratio, while few microbial genera were altered in the HF or HFS group.
31336215	7	46	theme	bile	1140:1143	arg1	metabolism					1150:1159	bile acid metabolism	1140:1159	bile acid metabolism	1140:1159	Changes in microbial activity according to dietary treatment were also reflected in the disruption of short-chain fatty acid production and bile acid metabolism, which were mainly associated with fiber intake.
31336215	5	47	theme	HF	802:803	arg1	diets					813:817	both HF and HFS diets	797:817	both HF and HFS diets	797:817	The CAF diet strongly induced an obese phenotype accompanied by dyslipidemia, hyperleptinemia, insulin resistance and hepatic steatosis, whereas both HF and HFS diets promoted overweight.
31336215	8	48	theme	host-microbiota	1371:1385	arg1	co-metabolites					1387:1400	host-microbiota co-metabolites	1371:1400	host-microbiota co-metabolites	1371:1400	Urinary metabolomics revealed a significant increase in metabolites related to oxidative stress and metabolic inflammation together with an altered excretion of host-microbiota co-metabolites only in the CAF group.
31336215	9	49	theme	several	1435:1441	arg1	associations					1443:1454	several associations	1435:1454	several associations between metabolic patterns, physiological status and specific microbial communities	1435:1538	Moreover, several associations between metabolic patterns, physiological status and specific microbial communities were described, helping to elucidate the crucial role of the microbiota in host homeostasis.
31336215	3	50	theme	host-microbiota	464:478	arg1	co-metabolism					480:492	the host-microbiota co-metabolism	460:492	the host-microbiota co-metabolism	460:492	We aimed to characterize the physiological, microbial and metabolic changes induced by different obesogenic diets to understand the diet-specific modulation of the host-microbiota co-metabolism in rodents.
31336215	0	51	theme	diets	33:37	arg1	Impact					0:5	Impact	0:5	Impact of different hypercaloric diets on obesity features in rats	0:65	Impact of different hypercaloric diets on obesity features in rats: a metagenomics and metabolomics integrative approach.
31336215	4	52	theme	standard	545:552	arg1	diets					632:636	standard, cafeteria (CAF), low-fat (LF), high-fat (HF) and high-fat high-sucrose (HFS) diets	545:636	standard, cafeteria (CAF), low-fat (LF), high-fat (HF) and high-fat high-sucrose (HFS) diets	545:636	For this purpose, Wistar rats were fed standard, cafeteria (CAF), low-fat (LF), high-fat (HF) and high-fat high-sucrose (HFS) diets for 10 weeks.
31336215	7	53	theme	short-chain	1102:1112	arg1	acid					1120:1123	short-chain fatty acid	1102:1123	short-chain fatty acid production	1102:1134	Changes in microbial activity according to dietary treatment were also reflected in the disruption of short-chain fatty acid production and bile acid metabolism, which were mainly associated with fiber intake.
31336215	7	54	theme	fatty	1114:1118	arg1	acid					1120:1123	short-chain fatty acid	1102:1123	short-chain fatty acid production	1102:1134	Changes in microbial activity according to dietary treatment were also reflected in the disruption of short-chain fatty acid production and bile acid metabolism, which were mainly associated with fiber intake.
31336215	3	55	theme	co-metabolism	480:492	arg1	modulation					446:455	the diet-specific modulation	428:455	the diet-specific modulation of the host-microbiota co-metabolism in rodents	428:503	We aimed to characterize the physiological, microbial and metabolic changes induced by different obesogenic diets to understand the diet-specific modulation of the host-microbiota co-metabolism in rodents.
31336215	5	56	theme	insulin	747:753	arg1	resistance					755:764	insulin resistance	747:764	insulin resistance	747:764	The CAF diet strongly induced an obese phenotype accompanied by dyslipidemia, hyperleptinemia, insulin resistance and hepatic steatosis, whereas both HF and HFS diets promoted overweight.
31336215	7	57	theme	metabolism	1150:1159	arg1	disruption					1088:1097	the disruption	1084:1097	the disruption of short-chain fatty acid production and bile acid metabolism, which were mainly associated with fiber intake	1084:1207	Changes in microbial activity according to dietary treatment were also reflected in the disruption of short-chain fatty acid production and bile acid metabolism, which were mainly associated with fiber intake.
31336215	9	58	theme	physiological	1484:1496	arg1	status					1498:1503	physiological status	1484:1503	physiological status	1484:1503	Moreover, several associations between metabolic patterns, physiological status and specific microbial communities were described, helping to elucidate the crucial role of the microbiota in host homeostasis.
31336215	2	59	theme	communities	218:228	arg1	Dysbiosis					195:203	Dysbiosis	195:203	Dysbiosis of microbial communities	195:228	Dysbiosis of microbial communities contributes to the development of metabolic diseases such as obesity.
31336215	10	60	theme	gut	1728:1730	arg1	composition					1743:1753	gut microbiota composition	1728:1753	gut microbiota composition	1728:1753	Overall, our study suggests that different hypercaloric dietary models distinctively influence gut microbiota composition and reveals robust and similar clustering patterns concerning both cecal microbiome and urinary metabolome profiles.
31336215	0	61	theme	obesity	42:48	arg1	features					50:57	obesity features	42:57	obesity features in rats	42:65	Impact of different hypercaloric diets on obesity features in rats: a metagenomics and metabolomics integrative approach.
31336215	10	62	theme	clustering	1786:1795	arg1	patterns					1797:1804	robust and similar clustering patterns	1767:1804	robust and similar clustering patterns concerning both cecal microbiome and urinary metabolome profiles	1767:1869	Overall, our study suggests that different hypercaloric dietary models distinctively influence gut microbiota composition and reveals robust and similar clustering patterns concerning both cecal microbiome and urinary metabolome profiles.
31336215	6	63	theme	CAF	867:869	arg1	feeding					871:877	CAF feeding	867:877	CAF feeding	867:877	Concerning the microbiome, CAF feeding induced a rise of the Bacteroidetes-to-Firmicutes ratio, while few microbial genera were altered in the HF or HFS group.
31336215	2	64	theme	microbial	208:216	arg1	communities					218:228	microbial communities	208:228	microbial communities	208:228	Dysbiosis of microbial communities contributes to the development of metabolic diseases such as obesity.
31336215	8	65	theme	significant	1242:1252	arg1	increase					1254:1261	a significant increase	1240:1261	a significant increase in metabolites related to oxidative stress and metabolic inflammation	1240:1331	Urinary metabolomics revealed a significant increase in metabolites related to oxidative stress and metabolic inflammation together with an altered excretion of host-microbiota co-metabolites only in the CAF group.
31336215	8	66	theme	CAF	1414:1416	arg1	group					1418:1422	the CAF group	1410:1422	the CAF group	1410:1422	Urinary metabolomics revealed a significant increase in metabolites related to oxidative stress and metabolic inflammation together with an altered excretion of host-microbiota co-metabolites only in the CAF group.
31336215	9	67	theme	microbiota	1601:1610	arg1	role					1589:1592	the crucial role	1577:1592	the crucial role of the microbiota in host homeostasis	1577:1630	Moreover, several associations between metabolic patterns, physiological status and specific microbial communities were described, helping to elucidate the crucial role of the microbiota in host homeostasis.
31336215	9	68	theme	host	1615:1618	arg1	homeostasis					1620:1630	host homeostasis	1615:1630	host homeostasis	1615:1630	Moreover, several associations between metabolic patterns, physiological status and specific microbial communities were described, helping to elucidate the crucial role of the microbiota in host homeostasis.
31336215	2	69	theme	metabolic	264:272	arg1	diseases					274:281	metabolic diseases	264:281	metabolic diseases such as obesity	264:297	Dysbiosis of microbial communities contributes to the development of metabolic diseases such as obesity.
31336215	2	69	theme	metabolic	264:272	arg1	obesity					291:297	obesity	291:297	obesity	291:297	Dysbiosis of microbial communities contributes to the development of metabolic diseases such as obesity.
31336215	9	70	theme	microbial	1518:1526	arg1	communities					1528:1538	specific microbial communities	1509:1538	specific microbial communities	1509:1538	Moreover, several associations between metabolic patterns, physiological status and specific microbial communities were described, helping to elucidate the crucial role of the microbiota in host homeostasis.
31336215	9	71	theme	crucial	1581:1587	arg1	role					1589:1592	the crucial role	1577:1592	the crucial role of the microbiota in host homeostasis	1577:1630	Moreover, several associations between metabolic patterns, physiological status and specific microbial communities were described, helping to elucidate the crucial role of the microbiota in host homeostasis.
31336215	5	72	theme	obese	685:689	arg1	phenotype					691:699	an obese phenotype	682:699	an obese phenotype accompanied by dyslipidemia, hyperleptinemia, insulin resistance and hepatic steatosis	682:786	The CAF diet strongly induced an obese phenotype accompanied by dyslipidemia, hyperleptinemia, insulin resistance and hepatic steatosis, whereas both HF and HFS diets promoted overweight.
31336215	10	73	theme	robust	1767:1772	arg1	patterns					1797:1804	robust and similar clustering patterns	1767:1804	robust and similar clustering patterns concerning both cecal microbiome and urinary metabolome profiles	1767:1869	Overall, our study suggests that different hypercaloric dietary models distinctively influence gut microbiota composition and reveals robust and similar clustering patterns concerning both cecal microbiome and urinary metabolome profiles.
31336215	8	74	theme	metabolic	1310:1318	arg1	inflammation					1320:1331	metabolic inflammation	1310:1331	metabolic inflammation	1310:1331	Urinary metabolomics revealed a significant increase in metabolites related to oxidative stress and metabolic inflammation together with an altered excretion of host-microbiota co-metabolites only in the CAF group.
31336215	8	75	theme	related	1278:1284	arg1	metabolites					1266:1276	metabolites	1266:1276	metabolites related to oxidative stress and metabolic inflammation	1266:1331	Urinary metabolomics revealed a significant increase in metabolites related to oxidative stress and metabolic inflammation together with an altered excretion of host-microbiota co-metabolites only in the CAF group.
31336215	3	76	theme	physiological	329:341	arg1	changes					368:374	the physiological, microbial and metabolic changes	325:374	the physiological, microbial and metabolic changes induced by different obesogenic diets	325:412	We aimed to characterize the physiological, microbial and metabolic changes induced by different obesogenic diets to understand the diet-specific modulation of the host-microbiota co-metabolism in rodents.
31336215	4	77	theme	high-fat	604:611	arg1	HFS					627:629	HFS	627:629	HFS	627:629	For this purpose, Wistar rats were fed standard, cafeteria (CAF), low-fat (LF), high-fat (HF) and high-fat high-sucrose (HFS) diets for 10 weeks.
31336215	4	77	theme	high-fat	604:611	arg1	high-sucrose					613:624	high-fat high-sucrose	604:624	high-fat high-sucrose (HFS)	604:630	For this purpose, Wistar rats were fed standard, cafeteria (CAF), low-fat (LF), high-fat (HF) and high-fat high-sucrose (HFS) diets for 10 weeks.
31336215	3	78	from	modulation	446:455	arg1	rodents					497:503	rodents	497:503	rodents	497:503	We aimed to characterize the physiological, microbial and metabolic changes induced by different obesogenic diets to understand the diet-specific modulation of the host-microbiota co-metabolism in rodents.
31336215	9	79	theme	specific	1509:1516	arg1	communities					1528:1538	specific microbial communities	1509:1538	specific microbial communities	1509:1538	Moreover, several associations between metabolic patterns, physiological status and specific microbial communities were described, helping to elucidate the crucial role of the microbiota in host homeostasis.
31336215	9	80	from	role	1589:1592	arg1	homeostasis					1620:1630	host homeostasis	1615:1630	host homeostasis	1615:1630	Moreover, several associations between metabolic patterns, physiological status and specific microbial communities were described, helping to elucidate the crucial role of the microbiota in host homeostasis.
31336215	4	81	theme	Wistar	524:529	arg1	rats					531:534	Wistar rats	524:534	Wistar rats	524:534	For this purpose, Wistar rats were fed standard, cafeteria (CAF), low-fat (LF), high-fat (HF) and high-fat high-sucrose (HFS) diets for 10 weeks.
31336215	7	82	theme	acid	1120:1123	arg1	production					1125:1134	short-chain fatty acid production	1102:1134	short-chain fatty acid production	1102:1134	Changes in microbial activity according to dietary treatment were also reflected in the disruption of short-chain fatty acid production and bile acid metabolism, which were mainly associated with fiber intake.
31336215	6	83	theme	HF	983:984	arg1	group					993:997	the HF or HFS group	979:997	group	993:997	Concerning the microbiome, CAF feeding induced a rise of the Bacteroidetes-to-Firmicutes ratio, while few microbial genera were altered in the HF or HFS group.
30040061	2	0	theme	strain	116:121	arg1	195T					127:130	strain SMC 195T	116:130	strain SMC 195T	116:130	A novel Gram-stain-positive, aerobic actinomycete, designated strain SMC 195T, was isolated from soil collected from a mangrove forest in Thailand.
30040061	5	1	theme	Pseudonocardia	636:649	arg1	species					623:629	the most closely phylogenetically related species	581:629	the most closely phylogenetically related species	581:629	The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that SMC 195T represented a member of the genus Pseudonocardia, and the most closely phylogenetically related species were Pseudonocardia yuanmonensisJCM 18055T (99.2 % 16S rRNA gene sequence similarity), Pseudonocardia halophobicaNRRL B-16514T (98.9 %) and Pseudonocardia kujensisNRRL B-24890T (98.7 %).
30040061	5	1	theme	Pseudonocardia	636:649	arg1	similarity					705:714	99.2 % 16S rRNA gene sequence similarity	675:714	99.2 % 16S rRNA gene sequence similarity	675:714	The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that SMC 195T represented a member of the genus Pseudonocardia, and the most closely phylogenetically related species were Pseudonocardia yuanmonensisJCM 18055T (99.2 % 16S rRNA gene sequence similarity), Pseudonocardia halophobicaNRRL B-16514T (98.9 %) and Pseudonocardia kujensisNRRL B-24890T (98.7 %).
30040061	5	1	theme	Pseudonocardia	636:649	arg1	18055T					667:672	Pseudonocardia yuanmonensisJCM 18055T	636:672	Pseudonocardia yuanmonensisJCM 18055T (99.2 % 16S rRNA gene sequence similarity)	636:715	The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that SMC 195T represented a member of the genus Pseudonocardia, and the most closely phylogenetically related species were Pseudonocardia yuanmonensisJCM 18055T (99.2 % 16S rRNA gene sequence similarity), Pseudonocardia halophobicaNRRL B-16514T (98.9 %) and Pseudonocardia kujensisNRRL B-24890T (98.7 %).
30040061	4	2	theme	rod-shaped	316:325	arg1	elements					327:334	rod-shaped elements	316:334	rod-shaped elements	316:334	The substrate mycelium was fragmented into rod-shaped elements, and spore chains consisting of smooth and rod-shaped spores were formed on the aerial mycelium.
30040061	11	3	theme	branched	1230:1237	arg1	acid					1245:1248	the branched fatty acid iso-C16 : 0 (33.6 %)	1226:1269	the branched fatty acid iso-C16 : 0 (33.6 %)	1226:1269	The major cellular fatty acid was the branched fatty acid iso-C16 : 0 (33.6 %).
30040061	11	3	theme	branched	1230:1237	arg1	acid					1217:1220	The major cellular fatty acid	1192:1220	The major cellular fatty acid	1192:1220	The major cellular fatty acid was the branched fatty acid iso-C16 : 0 (33.6 %).
30040061	2	4	dep	Gram-stain-positive	62:80	arg1	aerobic					83:89	aerobic	83:89	aerobic	83:89	A novel Gram-stain-positive, aerobic actinomycete, designated strain SMC 195T, was isolated from soil collected from a mangrove forest in Thailand.
30040061	6	5	theme	related	907:913	arg1	species					915:921	the closest phylogenetically related species	878:921	SMC 195Tand the closest phylogenetically related species	866:921	However, the DNA-DNA relatedness values between SMC 195Tand the closest phylogenetically related species were significantly below 70 %.
30040061	5	6	theme	yuanmonensisJCM	651:665	arg1	species					623:629	the most closely phylogenetically related species	581:629	the most closely phylogenetically related species	581:629	The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that SMC 195T represented a member of the genus Pseudonocardia, and the most closely phylogenetically related species were Pseudonocardia yuanmonensisJCM 18055T (99.2 % 16S rRNA gene sequence similarity), Pseudonocardia halophobicaNRRL B-16514T (98.9 %) and Pseudonocardia kujensisNRRL B-24890T (98.7 %).
30040061	5	6	theme	yuanmonensisJCM	651:665	arg1	similarity					705:714	99.2 % 16S rRNA gene sequence similarity	675:714	99.2 % 16S rRNA gene sequence similarity	675:714	The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that SMC 195T represented a member of the genus Pseudonocardia, and the most closely phylogenetically related species were Pseudonocardia yuanmonensisJCM 18055T (99.2 % 16S rRNA gene sequence similarity), Pseudonocardia halophobicaNRRL B-16514T (98.9 %) and Pseudonocardia kujensisNRRL B-24890T (98.7 %).
30040061	5	6	theme	yuanmonensisJCM	651:665	arg1	18055T					667:672	Pseudonocardia yuanmonensisJCM 18055T	636:672	Pseudonocardia yuanmonensisJCM 18055T (99.2 % 16S rRNA gene sequence similarity)	636:715	The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that SMC 195T represented a member of the genus Pseudonocardia, and the most closely phylogenetically related species were Pseudonocardia yuanmonensisJCM 18055T (99.2 % 16S rRNA gene sequence similarity), Pseudonocardia halophobicaNRRL B-16514T (98.9 %) and Pseudonocardia kujensisNRRL B-24890T (98.7 %).
30040061	15	7	theme	type	1707:1710	arg1	strain					1712:1717	The type strain	1703:1717	The type strain	1703:1717	The type strain is SMC 195T (=TBRC 7778T=NBRC 113150T).
30040061	15	7	theme	type	1707:1710	arg1	195T					1726:1729	SMC 195T	1722:1729	SMC 195T (=TBRC 7778T=NBRC 113150T)	1722:1756	The type strain is SMC 195T (=TBRC 7778T=NBRC 113150T).
30040061	15	8	theme	SMC	1722:1724	arg1	113150T					1749:1755	=TBRC 7778T=NBRC 113150T	1732:1755	=TBRC 7778T=NBRC 113150T	1732:1755	The type strain is SMC 195T (=TBRC 7778T=NBRC 113150T).
30040061	15	8	theme	SMC	1722:1724	arg1	strain					1712:1717	The type strain	1703:1717	The type strain	1703:1717	The type strain is SMC 195T (=TBRC 7778T=NBRC 113150T).
30040061	15	8	theme	SMC	1722:1724	arg1	195T					1726:1729	SMC 195T	1722:1729	SMC 195T (=TBRC 7778T=NBRC 113150T)	1722:1756	The type strain is SMC 195T (=TBRC 7778T=NBRC 113150T).
30040061	8	9	theme	wall	1015:1018	arg1	peptidoglycan					1020:1032	The cell wall peptidoglycan	1006:1032	The cell wall peptidoglycan	1006:1032	The cell wall peptidoglycan contained meso-diaminopimelic acid.
30040061	6	10	theme	phylogenetically	890:905	arg1	species					915:921	the closest phylogenetically related species	878:921	SMC 195Tand the closest phylogenetically related species	866:921	However, the DNA-DNA relatedness values between SMC 195Tand the closest phylogenetically related species were significantly below 70 %.
30040061	4	11	theme	rod-shaped	379:388	arg1	spores					390:395	smooth and rod-shaped spores	368:395	smooth and rod-shaped spores	368:395	The substrate mycelium was fragmented into rod-shaped elements, and spore chains consisting of smooth and rod-shaped spores were formed on the aerial mycelium.
30040061	7	12	theme	genomic	977:983	arg1	DNA					985:987	the genomic DNA	973:987	the genomic DNA	973:987	The G+C content of the genomic DNA was 74±0.8 mol%.
30040061	5	13	theme	Pseudonocardia	718:731	arg1	B-16514T					749:756	Pseudonocardia halophobicaNRRL B-16514T	718:756	Pseudonocardia halophobicaNRRL B-16514T (98.9 %)	718:765	The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that SMC 195T represented a member of the genus Pseudonocardia, and the most closely phylogenetically related species were Pseudonocardia yuanmonensisJCM 18055T (99.2 % 16S rRNA gene sequence similarity), Pseudonocardia halophobicaNRRL B-16514T (98.9 %) and Pseudonocardia kujensisNRRL B-24890T (98.7 %).
30040061	5	13	theme	Pseudonocardia	718:731	arg1	%					764:764	98.9 %	759:764	98.9 %	759:764	The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that SMC 195T represented a member of the genus Pseudonocardia, and the most closely phylogenetically related species were Pseudonocardia yuanmonensisJCM 18055T (99.2 % 16S rRNA gene sequence similarity), Pseudonocardia halophobicaNRRL B-16514T (98.9 %) and Pseudonocardia kujensisNRRL B-24890T (98.7 %).
30040061	6	14	theme	closest	882:888	arg1	species					915:921	the closest phylogenetically related species	878:921	SMC 195Tand the closest phylogenetically related species	866:921	However, the DNA-DNA relatedness values between SMC 195Tand the closest phylogenetically related species were significantly below 70 %.
30040061	13	15	theme	genus	1616:1620	arg1	Pseudonocardia					1622:1635	the genus Pseudonocardia	1612:1635	the genus Pseudonocardia	1612:1635	On the basis of the results from phenotypic, chemotaxonomic and genotypic studies, it is concluded that SMC 195T represents a novel species of the genus Pseudonocardia, for which the name Pseudonocardia mangrovi sp.
30040061	4	16	theme	substrate	277:285	arg1	mycelium					287:294	The substrate mycelium	273:294	The substrate mycelium	273:294	The substrate mycelium was fragmented into rod-shaped elements, and spore chains consisting of smooth and rod-shaped spores were formed on the aerial mycelium.
30040061	5	17	theme	halophobicaNRRL	733:747	arg1	B-16514T					749:756	Pseudonocardia halophobicaNRRL B-16514T	718:756	Pseudonocardia halophobicaNRRL B-16514T (98.9 %)	718:765	The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that SMC 195T represented a member of the genus Pseudonocardia, and the most closely phylogenetically related species were Pseudonocardia yuanmonensisJCM 18055T (99.2 % 16S rRNA gene sequence similarity), Pseudonocardia halophobicaNRRL B-16514T (98.9 %) and Pseudonocardia kujensisNRRL B-24890T (98.7 %).
30040061	5	17	theme	halophobicaNRRL	733:747	arg1	%					764:764	98.9 %	759:764	98.9 %	759:764	The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that SMC 195T represented a member of the genus Pseudonocardia, and the most closely phylogenetically related species were Pseudonocardia yuanmonensisJCM 18055T (99.2 % 16S rRNA gene sequence similarity), Pseudonocardia halophobicaNRRL B-16514T (98.9 %) and Pseudonocardia kujensisNRRL B-24890T (98.7 %).
30040061	5	18	theme	16S	479:481	arg1	sequences					493:501	16S rRNA gene sequences	479:501	16S rRNA gene sequences	479:501	The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that SMC 195T represented a member of the genus Pseudonocardia, and the most closely phylogenetically related species were Pseudonocardia yuanmonensisJCM 18055T (99.2 % 16S rRNA gene sequence similarity), Pseudonocardia halophobicaNRRL B-16514T (98.9 %) and Pseudonocardia kujensisNRRL B-24890T (98.7 %).
30040061	3	19	theme	branched	234:241	arg1	substrate					243:251	extensively branched substrate	222:251	extensively branched substrate	222:251	The strain produced extensively branched substrate and aerial mycelia.
30040061	15	20	theme	=TBRC	1732:1736	arg1	113150T					1749:1755	=TBRC 7778T=NBRC 113150T	1732:1755	=TBRC 7778T=NBRC 113150T	1732:1755	The type strain is SMC 195T (=TBRC 7778T=NBRC 113150T).
30040061	15	20	theme	=TBRC	1732:1736	arg1	195T					1726:1729	SMC 195T	1722:1729	SMC 195T (=TBRC 7778T=NBRC 113150T)	1722:1756	The type strain is SMC 195T (=TBRC 7778T=NBRC 113150T).
30040061	13	21	theme	Pseudonocardia	1622:1635	arg1	species					1601:1607	a novel species	1593:1607	a novel species	1593:1607	On the basis of the results from phenotypic, chemotaxonomic and genotypic studies, it is concluded that SMC 195T represents a novel species of the genus Pseudonocardia, for which the name Pseudonocardia mangrovi sp.
30040061	5	22	theme	rRNA	483:486	arg1	sequences					493:501	16S rRNA gene sequences	479:501	16S rRNA gene sequences	479:501	The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that SMC 195T represented a member of the genus Pseudonocardia, and the most closely phylogenetically related species were Pseudonocardia yuanmonensisJCM 18055T (99.2 % 16S rRNA gene sequence similarity), Pseudonocardia halophobicaNRRL B-16514T (98.9 %) and Pseudonocardia kujensisNRRL B-24890T (98.7 %).
30040061	13	23	theme	name	1652:1655	arg1	sp					1681:1682	the name Pseudonocardia mangrovi sp	1648:1682	the name Pseudonocardia mangrovi sp	1648:1682	On the basis of the results from phenotypic, chemotaxonomic and genotypic studies, it is concluded that SMC 195T represents a novel species of the genus Pseudonocardia, for which the name Pseudonocardia mangrovi sp.
30040061	2	24	from	forest	182:187	arg1	Thailand					192:199	Thailand	192:199	Thailand	192:199	A novel Gram-stain-positive, aerobic actinomycete, designated strain SMC 195T, was isolated from soil collected from a mangrove forest in Thailand.
30040061	5	25	theme	related	615:621	arg1	species					623:629	the most closely phylogenetically related species	581:629	the most closely phylogenetically related species	581:629	The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that SMC 195T represented a member of the genus Pseudonocardia, and the most closely phylogenetically related species were Pseudonocardia yuanmonensisJCM 18055T (99.2 % 16S rRNA gene sequence similarity), Pseudonocardia halophobicaNRRL B-16514T (98.9 %) and Pseudonocardia kujensisNRRL B-24890T (98.7 %).
30040061	5	25	theme	related	615:621	arg1	18055T					667:672	Pseudonocardia yuanmonensisJCM 18055T	636:672	Pseudonocardia yuanmonensisJCM 18055T (99.2 % 16S rRNA gene sequence similarity)	636:715	The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that SMC 195T represented a member of the genus Pseudonocardia, and the most closely phylogenetically related species were Pseudonocardia yuanmonensisJCM 18055T (99.2 % 16S rRNA gene sequence similarity), Pseudonocardia halophobicaNRRL B-16514T (98.9 %) and Pseudonocardia kujensisNRRL B-24890T (98.7 %).
30040061	13	26	theme	chemotaxonomic	1514:1527	arg1	studies					1543:1549	phenotypic, chemotaxonomic and genotypic studies	1502:1549	phenotypic, chemotaxonomic and genotypic studies	1502:1549	On the basis of the results from phenotypic, chemotaxonomic and genotypic studies, it is concluded that SMC 195T represents a novel species of the genus Pseudonocardia, for which the name Pseudonocardia mangrovi sp.
30040061	13	27	theme	Pseudonocardia	1657:1670	arg1	sp					1681:1682	the name Pseudonocardia mangrovi sp	1648:1682	the name Pseudonocardia mangrovi sp	1648:1682	On the basis of the results from phenotypic, chemotaxonomic and genotypic studies, it is concluded that SMC 195T represents a novel species of the genus Pseudonocardia, for which the name Pseudonocardia mangrovi sp.
30040061	5	28	theme	gene	488:491	arg1	sequences					493:501	16S rRNA gene sequences	479:501	16S rRNA gene sequences	479:501	The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that SMC 195T represented a member of the genus Pseudonocardia, and the most closely phylogenetically related species were Pseudonocardia yuanmonensisJCM 18055T (99.2 % 16S rRNA gene sequence similarity), Pseudonocardia halophobicaNRRL B-16514T (98.9 %) and Pseudonocardia kujensisNRRL B-24890T (98.7 %).
30040061	0	29	theme	mangrovi	15:22	arg1	sp					24:25	Pseudonocardia mangrovi sp	0:25	Pseudonocardia mangrovi sp.	0:26	Pseudonocardia mangrovi sp.
30040061	6	30	theme	SMC	866:868	arg1	195Tand					870:876	SMC 195Tand the closest phylogenetically related species	866:921	SMC 195Tand the closest phylogenetically related species	866:921	However, the DNA-DNA relatedness values between SMC 195Tand the closest phylogenetically related species were significantly below 70 %.
30040061	5	31	theme	genus	555:559	arg1	Pseudonocardia					561:574	the genus Pseudonocardia	551:574	the genus Pseudonocardia	551:574	The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that SMC 195T represented a member of the genus Pseudonocardia, and the most closely phylogenetically related species were Pseudonocardia yuanmonensisJCM 18055T (99.2 % 16S rRNA gene sequence similarity), Pseudonocardia halophobicaNRRL B-16514T (98.9 %) and Pseudonocardia kujensisNRRL B-24890T (98.7 %).
30040061	11	32	theme	fatty	1239:1243	arg1	acid					1245:1248	the branched fatty acid iso-C16 : 0 (33.6 %)	1226:1269	the branched fatty acid iso-C16 : 0 (33.6 %)	1226:1269	The major cellular fatty acid was the branched fatty acid iso-C16 : 0 (33.6 %).
30040061	11	32	theme	fatty	1239:1243	arg1	acid					1217:1220	The major cellular fatty acid	1192:1220	The major cellular fatty acid	1192:1220	The major cellular fatty acid was the branched fatty acid iso-C16 : 0 (33.6 %).
30040061	13	33	theme	genotypic	1533:1541	arg1	studies					1543:1549	phenotypic, chemotaxonomic and genotypic studies	1502:1549	phenotypic, chemotaxonomic and genotypic studies	1502:1549	On the basis of the results from phenotypic, chemotaxonomic and genotypic studies, it is concluded that SMC 195T represents a novel species of the genus Pseudonocardia, for which the name Pseudonocardia mangrovi sp.
30040061	0	34	theme	Pseudonocardia	0:13	arg1	sp					24:25	Pseudonocardia mangrovi sp	0:25	Pseudonocardia mangrovi sp.	0:26	Pseudonocardia mangrovi sp.
30040061	2	35	theme	novel	56:60	arg1	actinomycete					91:102	A novel Gram-stain-positive, aerobic actinomycete	54:102	A novel Gram-stain-positive, aerobic actinomycete	54:102	A novel Gram-stain-positive, aerobic actinomycete, designated strain SMC 195T, was isolated from soil collected from a mangrove forest in Thailand.
30040061	5	36	theme	Pseudonocardia	561:574	arg1	member					541:546	a member	539:546	a member of the genus Pseudonocardia	539:574	The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that SMC 195T represented a member of the genus Pseudonocardia, and the most closely phylogenetically related species were Pseudonocardia yuanmonensisJCM 18055T (99.2 % 16S rRNA gene sequence similarity), Pseudonocardia halophobicaNRRL B-16514T (98.9 %) and Pseudonocardia kujensisNRRL B-24890T (98.7 %).
30040061	13	37	theme	novel	1595:1599	arg1	species					1601:1607	a novel species	1593:1607	a novel species	1593:1607	On the basis of the results from phenotypic, chemotaxonomic and genotypic studies, it is concluded that SMC 195T represents a novel species of the genus Pseudonocardia, for which the name Pseudonocardia mangrovi sp.
30040061	5	38	theme	phylogenetic	448:459	arg1	analysis					461:468	phylogenetic analysis	448:468	phylogenetic analysis based on 16S rRNA gene sequences	448:501	The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that SMC 195T represented a member of the genus Pseudonocardia, and the most closely phylogenetically related species were Pseudonocardia yuanmonensisJCM 18055T (99.2 % 16S rRNA gene sequence similarity), Pseudonocardia halophobicaNRRL B-16514T (98.9 %) and Pseudonocardia kujensisNRRL B-24890T (98.7 %).
30040061	8	39	theme	cell	1010:1013	arg1	peptidoglycan					1020:1032	The cell wall peptidoglycan	1006:1032	The cell wall peptidoglycan	1006:1032	The cell wall peptidoglycan contained meso-diaminopimelic acid.
30040061	5	40	theme	gene	691:694	arg1	18055T					667:672	Pseudonocardia yuanmonensisJCM 18055T	636:672	Pseudonocardia yuanmonensisJCM 18055T (99.2 % 16S rRNA gene sequence similarity)	636:715	The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that SMC 195T represented a member of the genus Pseudonocardia, and the most closely phylogenetically related species were Pseudonocardia yuanmonensisJCM 18055T (99.2 % 16S rRNA gene sequence similarity), Pseudonocardia halophobicaNRRL B-16514T (98.9 %) and Pseudonocardia kujensisNRRL B-24890T (98.7 %).
30040061	5	40	theme	gene	691:694	arg1	similarity					705:714	99.2 % 16S rRNA gene sequence similarity	675:714	99.2 % 16S rRNA gene sequence similarity	675:714	The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that SMC 195T represented a member of the genus Pseudonocardia, and the most closely phylogenetically related species were Pseudonocardia yuanmonensisJCM 18055T (99.2 % 16S rRNA gene sequence similarity), Pseudonocardia halophobicaNRRL B-16514T (98.9 %) and Pseudonocardia kujensisNRRL B-24890T (98.7 %).
30040061	4	41	theme	smooth	368:373	arg1	spores					390:395	smooth and rod-shaped spores	368:395	smooth and rod-shaped spores	368:395	The substrate mycelium was fragmented into rod-shaped elements, and spore chains consisting of smooth and rod-shaped spores were formed on the aerial mycelium.
30040061	5	42	theme	analysis	461:468	arg1	results					437:443	The results	433:443	The results of phylogenetic analysis based on 16S rRNA gene sequences	433:501	The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that SMC 195T represented a member of the genus Pseudonocardia, and the most closely phylogenetically related species were Pseudonocardia yuanmonensisJCM 18055T (99.2 % 16S rRNA gene sequence similarity), Pseudonocardia halophobicaNRRL B-16514T (98.9 %) and Pseudonocardia kujensisNRRL B-24890T (98.7 %).
30040061	12	43	theme	unidentified	1443:1454	arg1	glycolipids					1456:1466	unidentified glycolipids	1443:1466	unidentified glycolipids	1443:1466	The polar lipids detected were phosphatidylethanolamine, phosphatidylmethylethanolamine, hydroxyphosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol and unidentified glycolipids.
30040061	5	44	theme	sequence	696:703	arg1	18055T					667:672	Pseudonocardia yuanmonensisJCM 18055T	636:672	Pseudonocardia yuanmonensisJCM 18055T (99.2 % 16S rRNA gene sequence similarity)	636:715	The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that SMC 195T represented a member of the genus Pseudonocardia, and the most closely phylogenetically related species were Pseudonocardia yuanmonensisJCM 18055T (99.2 % 16S rRNA gene sequence similarity), Pseudonocardia halophobicaNRRL B-16514T (98.9 %) and Pseudonocardia kujensisNRRL B-24890T (98.7 %).
30040061	5	44	theme	sequence	696:703	arg1	similarity					705:714	99.2 % 16S rRNA gene sequence similarity	675:714	99.2 % 16S rRNA gene sequence similarity	675:714	The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that SMC 195T represented a member of the genus Pseudonocardia, and the most closely phylogenetically related species were Pseudonocardia yuanmonensisJCM 18055T (99.2 % 16S rRNA gene sequence similarity), Pseudonocardia halophobicaNRRL B-16514T (98.9 %) and Pseudonocardia kujensisNRRL B-24890T (98.7 %).
30040061	8	45	contain	contained	1034:1042	arg1	peptidoglycan					1020:1032	The cell wall peptidoglycan	1006:1032	The cell wall peptidoglycan	1006:1032	The cell wall peptidoglycan contained meso-diaminopimelic acid.
30040061	8	45	contain	contained	1034:1042	arg2	acid					1064:1067	meso-diaminopimelic acid	1044:1067	meso-diaminopimelic acid	1044:1067	The cell wall peptidoglycan contained meso-diaminopimelic acid.
30040061	11	46	theme	fatty	1211:1215	arg1	acid					1245:1248	the branched fatty acid iso-C16 : 0 (33.6 %)	1226:1269	the branched fatty acid iso-C16 : 0 (33.6 %)	1226:1269	The major cellular fatty acid was the branched fatty acid iso-C16 : 0 (33.6 %).
30040061	11	46	theme	fatty	1211:1215	arg1	acid					1217:1220	The major cellular fatty acid	1192:1220	The major cellular fatty acid	1192:1220	The major cellular fatty acid was the branched fatty acid iso-C16 : 0 (33.6 %).
30040061	11	47	theme	major	1196:1200	arg1	acid					1245:1248	the branched fatty acid iso-C16 : 0 (33.6 %)	1226:1269	the branched fatty acid iso-C16 : 0 (33.6 %)	1226:1269	The major cellular fatty acid was the branched fatty acid iso-C16 : 0 (33.6 %).
30040061	11	47	theme	major	1196:1200	arg1	acid					1217:1220	The major cellular fatty acid	1192:1220	The major cellular fatty acid	1192:1220	The major cellular fatty acid was the branched fatty acid iso-C16 : 0 (33.6 %).
30040061	13	48	theme	phenotypic	1502:1511	arg1	studies					1543:1549	phenotypic, chemotaxonomic and genotypic studies	1502:1549	phenotypic, chemotaxonomic and genotypic studies	1502:1549	On the basis of the results from phenotypic, chemotaxonomic and genotypic studies, it is concluded that SMC 195T represents a novel species of the genus Pseudonocardia, for which the name Pseudonocardia mangrovi sp.
30040061	5	49	theme	Pseudonocardia	771:784	arg1	B-24890T					799:806	Pseudonocardia kujensisNRRL B-24890T	771:806	Pseudonocardia kujensisNRRL B-24890T (98.7 %)	771:815	The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that SMC 195T represented a member of the genus Pseudonocardia, and the most closely phylogenetically related species were Pseudonocardia yuanmonensisJCM 18055T (99.2 % 16S rRNA gene sequence similarity), Pseudonocardia halophobicaNRRL B-16514T (98.9 %) and Pseudonocardia kujensisNRRL B-24890T (98.7 %).
30040061	5	49	theme	Pseudonocardia	771:784	arg1	%					814:814	98.7 %	809:814	98.7 %	809:814	The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that SMC 195T represented a member of the genus Pseudonocardia, and the most closely phylogenetically related species were Pseudonocardia yuanmonensisJCM 18055T (99.2 % 16S rRNA gene sequence similarity), Pseudonocardia halophobicaNRRL B-16514T (98.9 %) and Pseudonocardia kujensisNRRL B-24890T (98.7 %).
30040061	3	50	theme	aerial	257:262	arg1	mycelia					264:270	aerial mycelia	257:270	aerial mycelia	257:270	The strain produced extensively branched substrate and aerial mycelia.
30040061	6	51	theme	relatedness	839:849	arg1	values					851:856	the DNA-DNA relatedness values	827:856	the DNA-DNA relatedness values between SMC 195Tand the closest phylogenetically related species	827:921	However, the DNA-DNA relatedness values between SMC 195Tand the closest phylogenetically related species were significantly below 70 %.
30040061	13	52	theme	SMC	1573:1575	arg1	195T					1577:1580	SMC 195T	1573:1580	SMC 195T	1573:1580	On the basis of the results from phenotypic, chemotaxonomic and genotypic studies, it is concluded that SMC 195T represents a novel species of the genus Pseudonocardia, for which the name Pseudonocardia mangrovi sp.
30040061	5	53	theme	kujensisNRRL	786:797	arg1	B-24890T					799:806	Pseudonocardia kujensisNRRL B-24890T	771:806	Pseudonocardia kujensisNRRL B-24890T (98.7 %)	771:815	The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that SMC 195T represented a member of the genus Pseudonocardia, and the most closely phylogenetically related species were Pseudonocardia yuanmonensisJCM 18055T (99.2 % 16S rRNA gene sequence similarity), Pseudonocardia halophobicaNRRL B-16514T (98.9 %) and Pseudonocardia kujensisNRRL B-24890T (98.7 %).
30040061	5	53	theme	kujensisNRRL	786:797	arg1	%					814:814	98.7 %	809:814	98.7 %	809:814	The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that SMC 195T represented a member of the genus Pseudonocardia, and the most closely phylogenetically related species were Pseudonocardia yuanmonensisJCM 18055T (99.2 % 16S rRNA gene sequence similarity), Pseudonocardia halophobicaNRRL B-16514T (98.9 %) and Pseudonocardia kujensisNRRL B-24890T (98.7 %).
30040061	5	54	theme	99.2 	675:679	arg1	%					680:680	%	680:680	%	680:680	The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that SMC 195T represented a member of the genus Pseudonocardia, and the most closely phylogenetically related species were Pseudonocardia yuanmonensisJCM 18055T (99.2 % 16S rRNA gene sequence similarity), Pseudonocardia halophobicaNRRL B-16514T (98.9 %) and Pseudonocardia kujensisNRRL B-24890T (98.7 %).
30040061	6	55	theme	DNA-DNA	831:837	arg1	values					851:856	the DNA-DNA relatedness values	827:856	the DNA-DNA relatedness values between SMC 195Tand the closest phylogenetically related species	827:921	However, the DNA-DNA relatedness values between SMC 195Tand the closest phylogenetically related species were significantly below 70 %.
30040061	11	56	theme	cellular	1202:1209	arg1	acid					1245:1248	the branched fatty acid iso-C16 : 0 (33.6 %)	1226:1269	the branched fatty acid iso-C16 : 0 (33.6 %)	1226:1269	The major cellular fatty acid was the branched fatty acid iso-C16 : 0 (33.6 %).
30040061	11	56	theme	cellular	1202:1209	arg1	acid					1217:1220	The major cellular fatty acid	1192:1220	The major cellular fatty acid	1192:1220	The major cellular fatty acid was the branched fatty acid iso-C16 : 0 (33.6 %).
30040061	5	57	theme	%	680:680	arg1	18055T					667:672	Pseudonocardia yuanmonensisJCM 18055T	636:672	Pseudonocardia yuanmonensisJCM 18055T (99.2 % 16S rRNA gene sequence similarity)	636:715	The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that SMC 195T represented a member of the genus Pseudonocardia, and the most closely phylogenetically related species were Pseudonocardia yuanmonensisJCM 18055T (99.2 % 16S rRNA gene sequence similarity), Pseudonocardia halophobicaNRRL B-16514T (98.9 %) and Pseudonocardia kujensisNRRL B-24890T (98.7 %).
30040061	5	57	theme	%	680:680	arg1	similarity					705:714	99.2 % 16S rRNA gene sequence similarity	675:714	99.2 % 16S rRNA gene sequence similarity	675:714	The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that SMC 195T represented a member of the genus Pseudonocardia, and the most closely phylogenetically related species were Pseudonocardia yuanmonensisJCM 18055T (99.2 % 16S rRNA gene sequence similarity), Pseudonocardia halophobicaNRRL B-16514T (98.9 %) and Pseudonocardia kujensisNRRL B-24890T (98.7 %).
30040061	8	58	theme	meso-diaminopimelic	1044:1062	arg1	acid					1064:1067	meso-diaminopimelic acid	1044:1067	meso-diaminopimelic acid	1044:1067	The cell wall peptidoglycan contained meso-diaminopimelic acid.
30040061	7	59	theme	74±0.8 mol	993:1002	arg1	%					1003:1003	74±0.8 mol%	993:1003	74±0.8 mol%	993:1003	The G+C content of the genomic DNA was 74±0.8 mol%.
30040061	7	59	theme	74±0.8 mol	993:1002	arg1	G+C content					958:968	The G+C content	954:968	The G+C content of the genomic DNA	954:987	The G+C content of the genomic DNA was 74±0.8 mol%.
30040061	2	60	attach	isolated	137:144	arg2	actinomycete					91:102	A novel Gram-stain-positive, aerobic actinomycete	54:102	A novel Gram-stain-positive, aerobic actinomycete	54:102	A novel Gram-stain-positive, aerobic actinomycete, designated strain SMC 195T, was isolated from soil collected from a mangrove forest in Thailand.
30040061	2	60	attach	isolated	137:144	arg1	soil					151:154	soil	151:154	soil collected from a mangrove forest in Thailand	151:199	A novel Gram-stain-positive, aerobic actinomycete, designated strain SMC 195T, was isolated from soil collected from a mangrove forest in Thailand.
30040061	2	61	theme	Gram-stain-positive	62:80	arg1	actinomycete					91:102	A novel Gram-stain-positive, aerobic actinomycete	54:102	A novel Gram-stain-positive, aerobic actinomycete	54:102	A novel Gram-stain-positive, aerobic actinomycete, designated strain SMC 195T, was isolated from soil collected from a mangrove forest in Thailand.
30040061	5	62	theme	16S	682:684	arg1	18055T					667:672	Pseudonocardia yuanmonensisJCM 18055T	636:672	Pseudonocardia yuanmonensisJCM 18055T (99.2 % 16S rRNA gene sequence similarity)	636:715	The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that SMC 195T represented a member of the genus Pseudonocardia, and the most closely phylogenetically related species were Pseudonocardia yuanmonensisJCM 18055T (99.2 % 16S rRNA gene sequence similarity), Pseudonocardia halophobicaNRRL B-16514T (98.9 %) and Pseudonocardia kujensisNRRL B-24890T (98.7 %).
30040061	5	62	theme	16S	682:684	arg1	similarity					705:714	99.2 % 16S rRNA gene sequence similarity	675:714	99.2 % 16S rRNA gene sequence similarity	675:714	The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that SMC 195T represented a member of the genus Pseudonocardia, and the most closely phylogenetically related species were Pseudonocardia yuanmonensisJCM 18055T (99.2 % 16S rRNA gene sequence similarity), Pseudonocardia halophobicaNRRL B-16514T (98.9 %) and Pseudonocardia kujensisNRRL B-24890T (98.7 %).
30040061	4	63	theme	spore	341:345	arg1	chains					347:352	spore chains	341:352	spore chains consisting of smooth and rod-shaped spores	341:395	The substrate mycelium was fragmented into rod-shaped elements, and spore chains consisting of smooth and rod-shaped spores were formed on the aerial mycelium.
30040061	6	64	dep	195Tand	870:876	arg1	species					915:921	the closest phylogenetically related species	878:921	SMC 195Tand the closest phylogenetically related species	866:921	However, the DNA-DNA relatedness values between SMC 195Tand the closest phylogenetically related species were significantly below 70 %.
30040061	5	65	theme	rRNA	686:689	arg1	18055T					667:672	Pseudonocardia yuanmonensisJCM 18055T	636:672	Pseudonocardia yuanmonensisJCM 18055T (99.2 % 16S rRNA gene sequence similarity)	636:715	The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that SMC 195T represented a member of the genus Pseudonocardia, and the most closely phylogenetically related species were Pseudonocardia yuanmonensisJCM 18055T (99.2 % 16S rRNA gene sequence similarity), Pseudonocardia halophobicaNRRL B-16514T (98.9 %) and Pseudonocardia kujensisNRRL B-24890T (98.7 %).
30040061	5	65	theme	rRNA	686:689	arg1	similarity					705:714	99.2 % 16S rRNA gene sequence similarity	675:714	99.2 % 16S rRNA gene sequence similarity	675:714	The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that SMC 195T represented a member of the genus Pseudonocardia, and the most closely phylogenetically related species were Pseudonocardia yuanmonensisJCM 18055T (99.2 % 16S rRNA gene sequence similarity), Pseudonocardia halophobicaNRRL B-16514T (98.9 %) and Pseudonocardia kujensisNRRL B-24890T (98.7 %).
30040061	2	66	theme	mangrove	173:180	arg1	forest					182:187	a mangrove forest	171:187	a mangrove forest in Thailand	171:199	A novel Gram-stain-positive, aerobic actinomycete, designated strain SMC 195T, was isolated from soil collected from a mangrove forest in Thailand.
30040061	7	67	theme	DNA	985:987	arg1	%					1003:1003	74±0.8 mol%	993:1003	74±0.8 mol%	993:1003	The G+C content of the genomic DNA was 74±0.8 mol%.
30040061	7	67	theme	DNA	985:987	arg1	G+C content					958:968	The G+C content	954:968	The G+C content of the genomic DNA	954:987	The G+C content of the genomic DNA was 74±0.8 mol%.
30040061	15	68	theme	7778T=NBRC	1738:1747	arg1	113150T					1749:1755	=TBRC 7778T=NBRC 113150T	1732:1755	=TBRC 7778T=NBRC 113150T	1732:1755	The type strain is SMC 195T (=TBRC 7778T=NBRC 113150T).
30040061	15	68	theme	7778T=NBRC	1738:1747	arg1	195T					1726:1729	SMC 195T	1722:1729	SMC 195T (=TBRC 7778T=NBRC 113150T)	1722:1756	The type strain is SMC 195T (=TBRC 7778T=NBRC 113150T).
30040061	4	69	theme	aerial	416:421	arg1	mycelium					423:430	the aerial mycelium	412:430	the aerial mycelium	412:430	The substrate mycelium was fragmented into rod-shaped elements, and spore chains consisting of smooth and rod-shaped spores were formed on the aerial mycelium.
30040061	13	70	dep	results	1489:1495	arg1	basis					1476:1480	basis	1476:1480	basis	1476:1480	On the basis of the results from phenotypic, chemotaxonomic and genotypic studies, it is concluded that SMC 195T represents a novel species of the genus Pseudonocardia, for which the name Pseudonocardia mangrovi sp.
30040061	13	70	dep	results	1489:1495	arg1	the					1472:1474	the	1472:1474	the	1472:1474	On the basis of the results from phenotypic, chemotaxonomic and genotypic studies, it is concluded that SMC 195T represents a novel species of the genus Pseudonocardia, for which the name Pseudonocardia mangrovi sp.
30040061	13	71	from	studies	1543:1549	arg1	results					1489:1495	the results	1485:1495	the results from phenotypic, chemotaxonomic and genotypic studies	1485:1549	On the basis of the results from phenotypic, chemotaxonomic and genotypic studies, it is concluded that SMC 195T represents a novel species of the genus Pseudonocardia, for which the name Pseudonocardia mangrovi sp.
30040061	2	72	theme	SMC	123:125	arg1	195T					127:130	strain SMC 195T	116:130	strain SMC 195T	116:130	A novel Gram-stain-positive, aerobic actinomycete, designated strain SMC 195T, was isolated from soil collected from a mangrove forest in Thailand.
30040061	12	73	theme	polar	1276:1280	arg1	lipids					1282:1287	The polar lipids	1272:1287	The polar lipids detected	1272:1296	The polar lipids detected were phosphatidylethanolamine, phosphatidylmethylethanolamine, hydroxyphosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol and unidentified glycolipids.
30040061	12	73	theme	polar	1276:1280	arg1	phosphatidylethanolamine					1303:1326	phosphatidylethanolamine	1303:1326	phosphatidylethanolamine	1303:1326	The polar lipids detected were phosphatidylethanolamine, phosphatidylmethylethanolamine, hydroxyphosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol and unidentified glycolipids.
30040061	5	74	theme	SMC	518:520	arg1	195T					522:525	SMC 195T	518:525	SMC 195T	518:525	The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that SMC 195T represented a member of the genus Pseudonocardia, and the most closely phylogenetically related species were Pseudonocardia yuanmonensisJCM 18055T (99.2 % 16S rRNA gene sequence similarity), Pseudonocardia halophobicaNRRL B-16514T (98.9 %) and Pseudonocardia kujensisNRRL B-24890T (98.7 %).
30040061	11	75	dep	acid	1245:1248	arg1	 0					1259:1260	 0	1259:1260	 0	1259:1260	The major cellular fatty acid was the branched fatty acid iso-C16 : 0 (33.6 %).
30040061	11	75	dep	acid	1245:1248	arg1	%					1268:1268	33.6 %	1263:1268	33.6 %	1263:1268	The major cellular fatty acid was the branched fatty acid iso-C16 : 0 (33.6 %).
30040061	9	76	theme	whole-cell	1074:1083	arg1	sugars					1085:1090	The whole-cell sugars	1070:1090	The whole-cell sugars	1070:1090	The whole-cell sugars consisted of arabinose, galactose, glucose, rhamnose and ribose.
29929396	12	0	theme	tendon	1864:1869	arg1	regeneration					1871:1882	MRL/MpJ tendon regeneration	1856:1882	MRL/MpJ tendon regeneration	1856:1882	CONCLUSION These findings support the tissue-driven hypothesis for MRL/MpJ tendon regeneration and motivate further investigation regarding specific roles of extracellular factors in scarless healing.
29929396	11	1	theme	improved	1680:1687	arg1	matrix					1689:1694	improved matrix and cell alignment	1680:1713	matrix	1689:1694	Supporting the regenerative role of the local environment, healing MRL/MpJ tendons exhibited improved matrix and cell alignment and a distinct composition of growth factors and Hyaluronan from C57Bl/6.
29929396	5	2	theme	environment	661:671	arg1	potential					631:639	the potential	627:639	the potential of the local tendon environment in driving scarless healing (1) by comparing the systemic response and the healing capacity associated with ear and tendon injuries in MRL/MpJ mice	627:819	Our objective is to investigate the potential of the local tendon environment in driving scarless healing (1) by comparing the systemic response and the healing capacity associated with ear and tendon injuries in MRL/MpJ mice, and (2) by comparing intrinsic healing properties between MRL/MpJ and normal healer C57Bl/6 tendons.
29929396	10	3	theme	comparable	1419:1428	arg1	responses					1443:1451	comparable inflammatory responses	1419:1451	comparable inflammatory responses	1419:1451	However, comparable inflammatory responses did not translate into analogous healing between tissues, suggesting that the systemic environment is not the driver of regeneration.
29929396	5	4	theme	driving	676:682	arg1	healing					693:699	driving scarless healing (1)	676:703	driving scarless healing (1) by comparing the systemic response and the healing capacity associated with ear and tendon injuries in MRL/MpJ mice	676:819	Our objective is to investigate the potential of the local tendon environment in driving scarless healing (1) by comparing the systemic response and the healing capacity associated with ear and tendon injuries in MRL/MpJ mice, and (2) by comparing intrinsic healing properties between MRL/MpJ and normal healer C57Bl/6 tendons.
29929396	12	5	theme	tissue-driven	1827:1839	arg1	hypothesis					1841:1850	the tissue-driven hypothesis	1823:1850	the tissue-driven hypothesis for MRL/MpJ tendon regeneration	1823:1882	CONCLUSION These findings support the tissue-driven hypothesis for MRL/MpJ tendon regeneration and motivate further investigation regarding specific roles of extracellular factors in scarless healing.
29929396	0	6	theme	Uncorrelated	0:11	arg1	response					21:28	Uncorrelated healing response	0:28	Uncorrelated healing response of tendon	0:38	Uncorrelated healing response of tendon and ear injuries in MRL highlight a role for the local tendon environment in driving scarless healing.
29929396	5	7	theme	local	648:652	arg1	environment					661:671	the local tendon environment	644:671	the local tendon environment	644:671	Our objective is to investigate the potential of the local tendon environment in driving scarless healing (1) by comparing the systemic response and the healing capacity associated with ear and tendon injuries in MRL/MpJ mice, and (2) by comparing intrinsic healing properties between MRL/MpJ and normal healer C57Bl/6 tendons.
29929396	1	8	theme	common	168:173	arg1	tears					158:162	PURPOSE Tendon tears	143:162	PURPOSE Tendon tears	143:162	PURPOSE Tendon tears are common injuries that heal with scar formation.
29929396	1	8	theme	common	168:173	arg1	injuries					175:182	common injuries	168:182	common injuries that heal with scar formation	168:212	PURPOSE Tendon tears are common injuries that heal with scar formation.
29929396	7	9	theme	Systemic	1065:1072	arg1	differences					1074:1084	Systemic differences	1065:1084	Systemic differences	1065:1084	Systemic differences were analyzed to assess effects of different injuries on the inflammatory response.
29929396	8	10	theme	tendon	1221:1226	arg1	injuries					1228:1235	tendon injuries	1221:1235	tendon injuries	1221:1235	Correlations were assessed between MRL/MpJ ear and tendon injuries to compare the extent of healing between regenerative tissues.
29929396	7	11	theme	different	1121:1129	arg1	injuries					1131:1138	different injuries	1121:1138	different injuries	1121:1138	Systemic differences were analyzed to assess effects of different injuries on the inflammatory response.
29929396	12	12	from	roles	1938:1942	arg1	healing					1981:1987	scarless healing	1972:1987	scarless healing	1972:1987	CONCLUSION These findings support the tissue-driven hypothesis for MRL/MpJ tendon regeneration and motivate further investigation regarding specific roles of extracellular factors in scarless healing.
29929396	0	13	theme	scarless	125:132	arg1	healing					134:140	scarless healing	125:140	scarless healing	125:140	Uncorrelated healing response of tendon and ear injuries in MRL highlight a role for the local tendon environment in driving scarless healing.
29929396	5	14	theme	healer	899:904	arg1	tendons					914:920	normal healer C57Bl/6 tendons	892:920	normal healer C57Bl/6 tendons	892:920	Our objective is to investigate the potential of the local tendon environment in driving scarless healing (1) by comparing the systemic response and the healing capacity associated with ear and tendon injuries in MRL/MpJ mice, and (2) by comparing intrinsic healing properties between MRL/MpJ and normal healer C57Bl/6 tendons.
29929396	6	15	theme	tendon	1014:1019	arg1	injuries					1027:1034	tendon punch injuries	1014:1034	tendon punch injuries	1014:1034	METHODS We examined the systemic inflammatory and local structural environments of ear and tendon punch injuries in MRL/MpJ and C57Bl/6 mice.
29929396	6	16	dep	inflammatory	956:967	arg1	the					943:945	the	943:945	the	943:945	METHODS We examined the systemic inflammatory and local structural environments of ear and tendon punch injuries in MRL/MpJ and C57Bl/6 mice.
29929396	6	17	theme	ear	1006:1008	arg1	inflammatory					956:967	inflammatory	956:967	inflammatory	956:967	METHODS We examined the systemic inflammatory and local structural environments of ear and tendon punch injuries in MRL/MpJ and C57Bl/6 mice.
29929396	0	18	from	response	21:28	arg1	MRL					60:62	MRL	60:62	MRL	60:62	Uncorrelated healing response of tendon and ear injuries in MRL highlight a role for the local tendon environment in driving scarless healing.
29929396	1	19	theme	PURPOSE	143:149	arg1	tears					158:162	PURPOSE Tendon tears	143:162	PURPOSE Tendon tears	143:162	PURPOSE Tendon tears are common injuries that heal with scar formation.
29929396	1	19	theme	PURPOSE	143:149	arg1	injuries					175:182	common injuries	168:182	common injuries that heal with scar formation	168:212	PURPOSE Tendon tears are common injuries that heal with scar formation.
29929396	6	20	theme	local	973:977	arg1	environments					990:1001	local structural environments	973:1001	local structural environments	973:1001	METHODS We examined the systemic inflammatory and local structural environments of ear and tendon punch injuries in MRL/MpJ and C57Bl/6 mice.
29929396	3	21	theme	scarless	325:332	arg1	healing					334:340	scarless healing	325:340	scarless healing	325:340	Most hypotheses regarding scarless healing implicate the systemic environment.
29929396	4	22	theme	sub-rupture	490:500	arg1	injuries					509:516	sub-rupture tendon injuries	490:516	sub-rupture tendon injuries	490:516	However, the tissue-specificity of this regenerative response and our previous findings showing regeneration of sub-rupture tendon injuries, which lack an overt systemic response, motivate a tissue-driven hypothesis.
29929396	5	23	theme	MRL/MpJ	808:814	arg1	mice					816:819	MRL/MpJ mice	808:819	MRL/MpJ mice	808:819	Our objective is to investigate the potential of the local tendon environment in driving scarless healing (1) by comparing the systemic response and the healing capacity associated with ear and tendon injuries in MRL/MpJ mice, and (2) by comparing intrinsic healing properties between MRL/MpJ and normal healer C57Bl/6 tendons.
29929396	4	24	theme	response	431:438	arg1	findings					457:464	our previous findings	444:464	our previous findings showing regeneration of sub-rupture tendon injuries	444:516	However, the tissue-specificity of this regenerative response and our previous findings showing regeneration of sub-rupture tendon injuries, which lack an overt systemic response, motivate a tissue-driven hypothesis.
29929396	4	24	theme	response	431:438	arg1	tissue-specificity					391:408	the tissue-specificity	387:408	the tissue-specificity of this regenerative response	387:438	However, the tissue-specificity of this regenerative response and our previous findings showing regeneration of sub-rupture tendon injuries, which lack an overt systemic response, motivate a tissue-driven hypothesis.
29929396	6	25	theme	C57Bl/6	1051:1057	arg1	mice					1059:1062	MRL/MpJ and C57Bl/6 mice	1039:1062	MRL/MpJ and C57Bl/6 mice	1039:1062	METHODS We examined the systemic inflammatory and local structural environments of ear and tendon punch injuries in MRL/MpJ and C57Bl/6 mice.
29929396	3	26	theme	systemic	356:363	arg1	environment					365:375	the systemic environment	352:375	the systemic environment	352:375	Most hypotheses regarding scarless healing implicate the systemic environment.
29929396	5	27	from	potential	631:639	arg1	healing					693:699	driving scarless healing (1)	676:703	driving scarless healing (1) by comparing the systemic response and the healing capacity associated with ear and tendon injuries in MRL/MpJ mice	676:819	Our objective is to investigate the potential of the local tendon environment in driving scarless healing (1) by comparing the systemic response and the healing capacity associated with ear and tendon injuries in MRL/MpJ mice, and (2) by comparing intrinsic healing properties between MRL/MpJ and normal healer C57Bl/6 tendons.
29929396	12	28	theme	extracellular	1947:1959	arg1	factors					1961:1967	extracellular factors	1947:1967	extracellular factors	1947:1967	CONCLUSION These findings support the tissue-driven hypothesis for MRL/MpJ tendon regeneration and motivate further investigation regarding specific roles of extracellular factors in scarless healing.
29929396	2	29	from	heal	243:246	arg1	tissues					272:278	several tissues	264:278	several tissues	264:278	Interestingly, MRL/MpJ mice heal without scar in several tissues, including tendon.
29929396	2	29	from	heal	243:246	arg1	tendon					291:296	tendon	291:296	tendon	291:296	Interestingly, MRL/MpJ mice heal without scar in several tissues, including tendon.
29929396	6	30	theme	MRL/MpJ	1039:1045	arg1	mice					1059:1062	MRL/MpJ and C57Bl/6 mice	1039:1062	MRL/MpJ and C57Bl/6 mice	1039:1062	METHODS We examined the systemic inflammatory and local structural environments of ear and tendon punch injuries in MRL/MpJ and C57Bl/6 mice.
29929396	11	31	theme	factors	1752:1758	arg1	Hyaluronan					1764:1773	Hyaluronan	1764:1773	Hyaluronan from C57Bl/6	1764:1786	Supporting the regenerative role of the local environment, healing MRL/MpJ tendons exhibited improved matrix and cell alignment and a distinct composition of growth factors and Hyaluronan from C57Bl/6.
29929396	11	31	theme	factors	1752:1758	arg1	composition					1730:1740	a distinct composition	1719:1740	a distinct composition of growth factors	1719:1758	Supporting the regenerative role of the local environment, healing MRL/MpJ tendons exhibited improved matrix and cell alignment and a distinct composition of growth factors and Hyaluronan from C57Bl/6.
29929396	11	31	theme	factors	1752:1758	arg1	matrix					1689:1694	improved matrix and cell alignment	1680:1713	matrix	1689:1694	Supporting the regenerative role of the local environment, healing MRL/MpJ tendons exhibited improved matrix and cell alignment and a distinct composition of growth factors and Hyaluronan from C57Bl/6.
29929396	11	31	theme	factors	1752:1758	arg1	alignment					1705:1713	improved matrix and cell alignment	1680:1713	alignment	1705:1713	Supporting the regenerative role of the local environment, healing MRL/MpJ tendons exhibited improved matrix and cell alignment and a distinct composition of growth factors and Hyaluronan from C57Bl/6.
29929396	0	32	theme	tendon	33:38	arg1	response					21:28	Uncorrelated healing response	0:28	Uncorrelated healing response of tendon	0:38	Uncorrelated healing response of tendon and ear injuries in MRL highlight a role for the local tendon environment in driving scarless healing.
29929396	0	32	theme	tendon	33:38	arg1	injuries					48:55	ear injuries	44:55	ear injuries in MRL	44:62	Uncorrelated healing response of tendon and ear injuries in MRL highlight a role for the local tendon environment in driving scarless healing.
29929396	9	33	theme	ear	1386:1388	arg1	injuries					1400:1407	ear or tendon injuries	1386:1407	injuries	1400:1407	RESULTS Analysis showed similarities between the systemic environment in MRL/MpJ post ear or tendon injuries.
29929396	7	34	theme	inflammatory	1147:1158	arg1	response					1160:1167	the inflammatory response	1143:1167	the inflammatory response	1143:1167	Systemic differences were analyzed to assess effects of different injuries on the inflammatory response.
29929396	6	35	theme	injuries	1027:1034	arg1	inflammatory					956:967	inflammatory	956:967	inflammatory	956:967	METHODS We examined the systemic inflammatory and local structural environments of ear and tendon punch injuries in MRL/MpJ and C57Bl/6 mice.
29929396	7	36	from	effects	1110:1116	arg1	response					1160:1167	the inflammatory response	1143:1167	the inflammatory response	1143:1167	Systemic differences were analyzed to assess effects of different injuries on the inflammatory response.
29929396	0	37	theme	ear	44:46	arg1	injuries					48:55	ear injuries	44:55	ear injuries in MRL	44:62	Uncorrelated healing response of tendon and ear injuries in MRL highlight a role for the local tendon environment in driving scarless healing.
29929396	9	38	theme	tendon	1393:1398	arg1	injuries					1400:1407	ear or tendon injuries	1386:1407	injuries	1400:1407	RESULTS Analysis showed similarities between the systemic environment in MRL/MpJ post ear or tendon injuries.
29929396	4	39	theme	systemic	539:546	arg1	response					548:555	an overt systemic response	530:555	an overt systemic response	530:555	However, the tissue-specificity of this regenerative response and our previous findings showing regeneration of sub-rupture tendon injuries, which lack an overt systemic response, motivate a tissue-driven hypothesis.
29929396	1	40	dep	heal	189:192	arg1	tears					158:162	PURPOSE Tendon tears	143:162	PURPOSE Tendon tears	143:162	PURPOSE Tendon tears are common injuries that heal with scar formation.
29929396	1	40	dep	heal	189:192	arg1	injuries					175:182	common injuries	168:182	common injuries that heal with scar formation	168:212	PURPOSE Tendon tears are common injuries that heal with scar formation.
29929396	11	41	theme	local	1627:1631	arg1	environment					1633:1643	the local environment	1623:1643	the local environment	1623:1643	Supporting the regenerative role of the local environment, healing MRL/MpJ tendons exhibited improved matrix and cell alignment and a distinct composition of growth factors and Hyaluronan from C57Bl/6.
29929396	1	42	theme	scar	199:202	arg1	formation					204:212	scar formation	199:212	scar formation	199:212	PURPOSE Tendon tears are common injuries that heal with scar formation.
29929396	12	43	theme	MRL/MpJ	1856:1862	arg1	regeneration					1871:1882	MRL/MpJ tendon regeneration	1856:1882	MRL/MpJ tendon regeneration	1856:1882	CONCLUSION These findings support the tissue-driven hypothesis for MRL/MpJ tendon regeneration and motivate further investigation regarding specific roles of extracellular factors in scarless healing.
29929396	12	44	theme	factors	1961:1967	arg1	roles					1938:1942	specific roles	1929:1942	specific roles of extracellular factors in scarless healing	1929:1987	CONCLUSION These findings support the tissue-driven hypothesis for MRL/MpJ tendon regeneration and motivate further investigation regarding specific roles of extracellular factors in scarless healing.
29929396	7	45	theme	injuries	1131:1138	arg1	effects					1110:1116	effects	1110:1116	effects of different injuries on the inflammatory response	1110:1167	Systemic differences were analyzed to assess effects of different injuries on the inflammatory response.
29929396	10	46	theme	regeneration	1573:1584	arg1	environment					1540:1550	the systemic environment	1527:1550	the systemic environment	1527:1550	However, comparable inflammatory responses did not translate into analogous healing between tissues, suggesting that the systemic environment is not the driver of regeneration.
29929396	10	46	theme	regeneration	1573:1584	arg1	driver					1563:1568	the driver	1559:1568	the driver of regeneration	1559:1584	However, comparable inflammatory responses did not translate into analogous healing between tissues, suggesting that the systemic environment is not the driver of regeneration.
29929396	11	47	theme	cell	1700:1703	arg1	alignment					1705:1713	improved matrix and cell alignment	1680:1713	alignment	1705:1713	Supporting the regenerative role of the local environment, healing MRL/MpJ tendons exhibited improved matrix and cell alignment and a distinct composition of growth factors and Hyaluronan from C57Bl/6.
29929396	5	48	theme	tendon	654:659	arg1	environment					661:671	the local tendon environment	644:671	the local tendon environment	644:671	Our objective is to investigate the potential of the local tendon environment in driving scarless healing (1) by comparing the systemic response and the healing capacity associated with ear and tendon injuries in MRL/MpJ mice, and (2) by comparing intrinsic healing properties between MRL/MpJ and normal healer C57Bl/6 tendons.
29929396	11	49	theme	MRL/MpJ	1654:1660	arg1	tendons					1662:1668	healing MRL/MpJ tendons	1646:1668	healing MRL/MpJ tendons	1646:1668	Supporting the regenerative role of the local environment, healing MRL/MpJ tendons exhibited improved matrix and cell alignment and a distinct composition of growth factors and Hyaluronan from C57Bl/6.
29929396	4	50	theme	injuries	509:516	arg1	regeneration					474:485	regeneration	474:485	regeneration of sub-rupture tendon injuries	474:516	However, the tissue-specificity of this regenerative response and our previous findings showing regeneration of sub-rupture tendon injuries, which lack an overt systemic response, motivate a tissue-driven hypothesis.
29929396	2	51	theme	several	264:270	arg1	tissues					272:278	several tissues	264:278	several tissues	264:278	Interestingly, MRL/MpJ mice heal without scar in several tissues, including tendon.
29929396	2	51	theme	several	264:270	arg1	tendon					291:296	tendon	291:296	tendon	291:296	Interestingly, MRL/MpJ mice heal without scar in several tissues, including tendon.
29929396	11	52	theme	distinct	1721:1728	arg1	composition					1730:1740	a distinct composition	1719:1740	a distinct composition of growth factors	1719:1758	Supporting the regenerative role of the local environment, healing MRL/MpJ tendons exhibited improved matrix and cell alignment and a distinct composition of growth factors and Hyaluronan from C57Bl/6.
29929396	1	53	theme	Tendon	151:156	arg1	tears					158:162	PURPOSE Tendon tears	143:162	PURPOSE Tendon tears	143:162	PURPOSE Tendon tears are common injuries that heal with scar formation.
29929396	1	53	theme	Tendon	151:156	arg1	injuries					175:182	common injuries	168:182	common injuries that heal with scar formation	168:212	PURPOSE Tendon tears are common injuries that heal with scar formation.
29929396	11	54	from	C57Bl/6	1780:1786	arg1	Hyaluronan					1764:1773	Hyaluronan	1764:1773	Hyaluronan from C57Bl/6	1764:1786	Supporting the regenerative role of the local environment, healing MRL/MpJ tendons exhibited improved matrix and cell alignment and a distinct composition of growth factors and Hyaluronan from C57Bl/6.
29929396	11	54	from	C57Bl/6	1780:1786	arg1	composition					1730:1740	a distinct composition	1719:1740	a distinct composition of growth factors	1719:1758	Supporting the regenerative role of the local environment, healing MRL/MpJ tendons exhibited improved matrix and cell alignment and a distinct composition of growth factors and Hyaluronan from C57Bl/6.
29929396	11	54	from	C57Bl/6	1780:1786	arg1	matrix					1689:1694	improved matrix and cell alignment	1680:1713	matrix	1689:1694	Supporting the regenerative role of the local environment, healing MRL/MpJ tendons exhibited improved matrix and cell alignment and a distinct composition of growth factors and Hyaluronan from C57Bl/6.
29929396	11	54	from	C57Bl/6	1780:1786	arg1	alignment					1705:1713	improved matrix and cell alignment	1680:1713	alignment	1705:1713	Supporting the regenerative role of the local environment, healing MRL/MpJ tendons exhibited improved matrix and cell alignment and a distinct composition of growth factors and Hyaluronan from C57Bl/6.
29929396	5	55	theme	intrinsic	843:851	arg1	properties					861:870	intrinsic healing properties	843:870	intrinsic healing properties	843:870	Our objective is to investigate the potential of the local tendon environment in driving scarless healing (1) by comparing the systemic response and the healing capacity associated with ear and tendon injuries in MRL/MpJ mice, and (2) by comparing intrinsic healing properties between MRL/MpJ and normal healer C57Bl/6 tendons.
29929396	10	56	theme	inflammatory	1430:1441	arg1	responses					1443:1451	comparable inflammatory responses	1419:1451	comparable inflammatory responses	1419:1451	However, comparable inflammatory responses did not translate into analogous healing between tissues, suggesting that the systemic environment is not the driver of regeneration.
29929396	8	57	theme	MRL/MpJ	1205:1211	arg1	ear					1213:1215	MRL/MpJ ear	1205:1215	MRL/MpJ ear	1205:1215	Correlations were assessed between MRL/MpJ ear and tendon injuries to compare the extent of healing between regenerative tissues.
29929396	6	58	dep	METHODS	923:929	arg1	examined					934:941	examined	934:941	examined the systemic inflammatory and local structural environments of ear and tendon punch injuries in MRL/MpJ and C57Bl/6 mice	934:1062	METHODS We examined the systemic inflammatory and local structural environments of ear and tendon punch injuries in MRL/MpJ and C57Bl/6 mice.
29929396	8	59	theme	healing	1262:1268	arg1	extent					1252:1257	the extent	1248:1257	the extent of healing between regenerative tissues	1248:1297	Correlations were assessed between MRL/MpJ ear and tendon injuries to compare the extent of healing between regenerative tissues.
29929396	5	60	theme	healing	853:859	arg1	properties					861:870	intrinsic healing properties	843:870	intrinsic healing properties	843:870	Our objective is to investigate the potential of the local tendon environment in driving scarless healing (1) by comparing the systemic response and the healing capacity associated with ear and tendon injuries in MRL/MpJ mice, and (2) by comparing intrinsic healing properties between MRL/MpJ and normal healer C57Bl/6 tendons.
29929396	8	61	theme	regenerative	1278:1289	arg1	tissues					1291:1297	regenerative tissues	1278:1297	regenerative tissues	1278:1297	Correlations were assessed between MRL/MpJ ear and tendon injuries to compare the extent of healing between regenerative tissues.
29929396	5	62	theme	scarless	684:691	arg1	healing					693:699	driving scarless healing (1)	676:703	driving scarless healing (1) by comparing the systemic response and the healing capacity associated with ear and tendon injuries in MRL/MpJ mice	676:819	Our objective is to investigate the potential of the local tendon environment in driving scarless healing (1) by comparing the systemic response and the healing capacity associated with ear and tendon injuries in MRL/MpJ mice, and (2) by comparing intrinsic healing properties between MRL/MpJ and normal healer C57Bl/6 tendons.
29929396	5	63	theme	normal	892:897	arg1	tendons					914:920	normal healer C57Bl/6 tendons	892:920	normal healer C57Bl/6 tendons	892:920	Our objective is to investigate the potential of the local tendon environment in driving scarless healing (1) by comparing the systemic response and the healing capacity associated with ear and tendon injuries in MRL/MpJ mice, and (2) by comparing intrinsic healing properties between MRL/MpJ and normal healer C57Bl/6 tendons.
29929396	4	64	theme	regenerative	418:429	arg1	response					431:438	this regenerative response	413:438	this regenerative response	413:438	However, the tissue-specificity of this regenerative response and our previous findings showing regeneration of sub-rupture tendon injuries, which lack an overt systemic response, motivate a tissue-driven hypothesis.
29929396	5	65	from	injuries	796:803	arg1	mice					816:819	MRL/MpJ mice	808:819	MRL/MpJ mice	808:819	Our objective is to investigate the potential of the local tendon environment in driving scarless healing (1) by comparing the systemic response and the healing capacity associated with ear and tendon injuries in MRL/MpJ mice, and (2) by comparing intrinsic healing properties between MRL/MpJ and normal healer C57Bl/6 tendons.
29929396	11	66	theme	regenerative	1602:1613	arg1	role					1615:1618	the regenerative role	1598:1618	the regenerative role of the local environment	1598:1643	Supporting the regenerative role of the local environment, healing MRL/MpJ tendons exhibited improved matrix and cell alignment and a distinct composition of growth factors and Hyaluronan from C57Bl/6.
29929396	5	67	theme	C57Bl/6	906:912	arg1	tendons					914:920	normal healer C57Bl/6 tendons	892:920	normal healer C57Bl/6 tendons	892:920	Our objective is to investigate the potential of the local tendon environment in driving scarless healing (1) by comparing the systemic response and the healing capacity associated with ear and tendon injuries in MRL/MpJ mice, and (2) by comparing intrinsic healing properties between MRL/MpJ and normal healer C57Bl/6 tendons.
29929396	6	68	theme	punch	1021:1025	arg1	injuries					1027:1034	tendon punch injuries	1014:1034	tendon punch injuries	1014:1034	METHODS We examined the systemic inflammatory and local structural environments of ear and tendon punch injuries in MRL/MpJ and C57Bl/6 mice.
29929396	12	69	dep	CONCLUSION	1789:1798	arg1	motivate					1888:1895	motivate	1888:1895	motivate further investigation regarding specific roles of extracellular factors in scarless healing	1888:1987	CONCLUSION These findings support the tissue-driven hypothesis for MRL/MpJ tendon regeneration and motivate further investigation regarding specific roles of extracellular factors in scarless healing.
29929396	12	69	dep	CONCLUSION	1789:1798	arg1	support					1815:1821	support	1815:1821	support the tissue-driven hypothesis for MRL/MpJ tendon regeneration	1815:1882	CONCLUSION These findings support the tissue-driven hypothesis for MRL/MpJ tendon regeneration and motivate further investigation regarding specific roles of extracellular factors in scarless healing.
29929396	5	70	theme	healing	748:754	arg1	capacity					756:763	the healing capacity	744:763	the healing capacity associated with ear and tendon injuries in MRL/MpJ mice	744:819	Our objective is to investigate the potential of the local tendon environment in driving scarless healing (1) by comparing the systemic response and the healing capacity associated with ear and tendon injuries in MRL/MpJ mice, and (2) by comparing intrinsic healing properties between MRL/MpJ and normal healer C57Bl/6 tendons.
29929396	2	71	theme	MRL/MpJ	230:236	arg1	mice					238:241	MRL/MpJ mice	230:241	MRL/MpJ mice	230:241	Interestingly, MRL/MpJ mice heal without scar in several tissues, including tendon.
29929396	9	72	theme	RESULTS	1300:1306	arg1	Analysis					1308:1315	RESULTS Analysis	1300:1315	RESULTS Analysis	1300:1315	RESULTS Analysis showed similarities between the systemic environment in MRL/MpJ post ear or tendon injuries.
29929396	3	73	theme	Most	299:302	arg1	hypotheses					304:313	Most hypotheses	299:313	Most hypotheses regarding scarless healing	299:340	Most hypotheses regarding scarless healing implicate the systemic environment.
29929396	10	74	theme	analogous	1476:1484	arg1	healing					1486:1492	analogous healing	1476:1492	analogous healing between tissues	1476:1508	However, comparable inflammatory responses did not translate into analogous healing between tissues, suggesting that the systemic environment is not the driver of regeneration.
29929396	4	75	theme	previous	448:455	arg1	findings					457:464	our previous findings	444:464	our previous findings showing regeneration of sub-rupture tendon injuries	444:516	However, the tissue-specificity of this regenerative response and our previous findings showing regeneration of sub-rupture tendon injuries, which lack an overt systemic response, motivate a tissue-driven hypothesis.
29929396	5	76	theme	systemic	722:729	arg1	response					731:738	the systemic response	718:738	the systemic response	718:738	Our objective is to investigate the potential of the local tendon environment in driving scarless healing (1) by comparing the systemic response and the healing capacity associated with ear and tendon injuries in MRL/MpJ mice, and (2) by comparing intrinsic healing properties between MRL/MpJ and normal healer C57Bl/6 tendons.
29929396	6	77	theme	structural	979:988	arg1	environments					990:1001	local structural environments	973:1001	local structural environments	973:1001	METHODS We examined the systemic inflammatory and local structural environments of ear and tendon punch injuries in MRL/MpJ and C57Bl/6 mice.
29929396	0	78	from	injuries	48:55	arg1	MRL					60:62	MRL	60:62	MRL	60:62	Uncorrelated healing response of tendon and ear injuries in MRL highlight a role for the local tendon environment in driving scarless healing.
29929396	0	79	theme	local	89:93	arg1	environment					102:112	the local tendon environment	85:112	the local tendon environment	85:112	Uncorrelated healing response of tendon and ear injuries in MRL highlight a role for the local tendon environment in driving scarless healing.
29929396	4	80	theme	tendon	502:507	arg1	injuries					509:516	sub-rupture tendon injuries	490:516	sub-rupture tendon injuries	490:516	However, the tissue-specificity of this regenerative response and our previous findings showing regeneration of sub-rupture tendon injuries, which lack an overt systemic response, motivate a tissue-driven hypothesis.
29929396	0	81	theme	healing	13:19	arg1	response					21:28	Uncorrelated healing response	0:28	Uncorrelated healing response of tendon	0:38	Uncorrelated healing response of tendon and ear injuries in MRL highlight a role for the local tendon environment in driving scarless healing.
29929396	9	82	theme	systemic	1349:1356	arg1	environment					1358:1368	the systemic environment	1345:1368	the systemic environment in MRL/MpJ post ear or tendon injuries	1345:1407	RESULTS Analysis showed similarities between the systemic environment in MRL/MpJ post ear or tendon injuries.
29929396	9	83	from	environment	1358:1368	arg1	MRL/MpJ					1373:1379	MRL/MpJ	1373:1379	MRL/MpJ post ear or tendon injuries	1373:1407	RESULTS Analysis showed similarities between the systemic environment in MRL/MpJ post ear or tendon injuries.
29929396	1	84	with	heal	189:192	arg1	formation					204:212	scar formation	199:212	scar formation	199:212	PURPOSE Tendon tears are common injuries that heal with scar formation.
29929396	4	85	theme	tissue-driven	569:581	arg1	hypothesis					583:592	a tissue-driven hypothesis	567:592	a tissue-driven hypothesis	567:592	However, the tissue-specificity of this regenerative response and our previous findings showing regeneration of sub-rupture tendon injuries, which lack an overt systemic response, motivate a tissue-driven hypothesis.
29929396	10	86	theme	systemic	1531:1538	arg1	environment					1540:1550	the systemic environment	1527:1550	the systemic environment	1527:1550	However, comparable inflammatory responses did not translate into analogous healing between tissues, suggesting that the systemic environment is not the driver of regeneration.
29929396	10	86	theme	systemic	1531:1538	arg1	driver					1563:1568	the driver	1559:1568	the driver of regeneration	1559:1584	However, comparable inflammatory responses did not translate into analogous healing between tissues, suggesting that the systemic environment is not the driver of regeneration.
29929396	11	87	theme	growth	1745:1750	arg1	factors					1752:1758	growth factors	1745:1758	growth factors	1745:1758	Supporting the regenerative role of the local environment, healing MRL/MpJ tendons exhibited improved matrix and cell alignment and a distinct composition of growth factors and Hyaluronan from C57Bl/6.
29929396	5	88	theme	ear	781:783	arg1	injuries					796:803	ear and tendon injuries	781:803	ear and tendon injuries in MRL/MpJ mice	781:819	Our objective is to investigate the potential of the local tendon environment in driving scarless healing (1) by comparing the systemic response and the healing capacity associated with ear and tendon injuries in MRL/MpJ mice, and (2) by comparing intrinsic healing properties between MRL/MpJ and normal healer C57Bl/6 tendons.
29929396	12	89	theme	scarless	1972:1979	arg1	healing					1981:1987	scarless healing	1972:1987	scarless healing	1972:1987	CONCLUSION These findings support the tissue-driven hypothesis for MRL/MpJ tendon regeneration and motivate further investigation regarding specific roles of extracellular factors in scarless healing.
29929396	4	90	theme	overt	533:537	arg1	response					548:555	an overt systemic response	530:555	an overt systemic response	530:555	However, the tissue-specificity of this regenerative response and our previous findings showing regeneration of sub-rupture tendon injuries, which lack an overt systemic response, motivate a tissue-driven hypothesis.
29929396	11	91	theme	environment	1633:1643	arg1	role					1615:1618	the regenerative role	1598:1618	the regenerative role of the local environment	1598:1643	Supporting the regenerative role of the local environment, healing MRL/MpJ tendons exhibited improved matrix and cell alignment and a distinct composition of growth factors and Hyaluronan from C57Bl/6.
29929396	5	92	theme	tendon	789:794	arg1	injuries					796:803	ear and tendon injuries	781:803	ear and tendon injuries in MRL/MpJ mice	781:819	Our objective is to investigate the potential of the local tendon environment in driving scarless healing (1) by comparing the systemic response and the healing capacity associated with ear and tendon injuries in MRL/MpJ mice, and (2) by comparing intrinsic healing properties between MRL/MpJ and normal healer C57Bl/6 tendons.
29929396	12	93	theme	specific	1929:1936	arg1	roles					1938:1942	specific roles	1929:1942	specific roles of extracellular factors in scarless healing	1929:1987	CONCLUSION These findings support the tissue-driven hypothesis for MRL/MpJ tendon regeneration and motivate further investigation regarding specific roles of extracellular factors in scarless healing.
29929396	5	94	dep	comparing	833:841	arg1	2					827:827	2	827:827	2	827:827	Our objective is to investigate the potential of the local tendon environment in driving scarless healing (1) by comparing the systemic response and the healing capacity associated with ear and tendon injuries in MRL/MpJ mice, and (2) by comparing intrinsic healing properties between MRL/MpJ and normal healer C57Bl/6 tendons.
29929396	12	95	theme	further	1897:1903	arg1	investigation					1905:1917	further investigation	1897:1917	further investigation regarding specific roles of extracellular factors in scarless healing	1897:1987	CONCLUSION These findings support the tissue-driven hypothesis for MRL/MpJ tendon regeneration and motivate further investigation regarding specific roles of extracellular factors in scarless healing.
29929396	0	96	theme	tendon	95:100	arg1	environment					102:112	the local tendon environment	85:112	the local tendon environment	85:112	Uncorrelated healing response of tendon and ear injuries in MRL highlight a role for the local tendon environment in driving scarless healing.
29929396	11	97	theme	healing	1646:1652	arg1	tendons					1662:1668	healing MRL/MpJ tendons	1646:1668	healing MRL/MpJ tendons	1646:1668	Supporting the regenerative role of the local environment, healing MRL/MpJ tendons exhibited improved matrix and cell alignment and a distinct composition of growth factors and Hyaluronan from C57Bl/6.
31791401	12	0	theme	cells	2062:2066	arg1	usage					2036:2040	usage	2036:2040	usage of mesenchymal stem cells in therapeutic strategies for lung diseases	2036:2110	These findings have implications for understanding the regulation of lung repair and the potential for usage of mesenchymal stem cells in therapeutic strategies for lung diseases.
31791401	7	1	theme	airway	1132:1137	arg1	epithelium					1139:1148	the airway epithelium	1128:1148	the airway epithelium	1128:1148	RESULTS During injury repair, a subgroup of GFP-labeled Dermo1+ mesenchymal cells were found to contribute to normal repair of the airway epithelium and differentiated into Club cells, ciliated cells, and goblet cells.
31791401	3	2	theme	normal	536:541	arg1	composition					543:553	the normal composition	532:553	the normal composition of airway epithelium following injury	532:591	However, the contributions of endogenous mesenchymal cells to recruitment, expansion or differentiation of stem cells, and repair and reestablishment of the normal composition of airway epithelium following injury have not been addressed.
31791401	8	3	theme	stem	1291:1294	arg1	cells					1325:1329	Dermo1+ stem cell regenerating epithelial cells	1283:1329	Dermo1+ stem cell regenerating epithelial cells	1283:1329	In Club cell-specific naphthalene injury model, the process of Dermo1+ stem cell regenerating epithelial cells was dissected.
31791401	11	4	theme	various	1907:1913	arg1	cells					1926:1930	various epithelial cells	1907:1930	various epithelial cells	1907:1930	The Dermo1+ mesenchymal stem cell differentiated into epithelial stem cells before reestablishing various epithelial cells.
31791401	4	5	theme	mesenchymal	654:664	arg1	cells					666:670	mouse pulmonary mesenchymal cells	638:670	mouse pulmonary mesenchymal cells	638:670	METHODS The role of mouse pulmonary mesenchymal cells was investigated by lineage tracing using Dermo1-Cre; ROSAmTmG mice.
31791401	7	6	theme	normal	1111:1116	arg1	repair					1118:1123	normal repair	1111:1123	normal repair of the airway epithelium	1111:1148	RESULTS During injury repair, a subgroup of GFP-labeled Dermo1+ mesenchymal cells were found to contribute to normal repair of the airway epithelium and differentiated into Club cells, ciliated cells, and goblet cells.
31791401	5	7	theme	GFP-labeled	818:828	arg1	cells					850:854	GFP-labeled Dermo1+ mesenchymal cells	818:854	GFP-labeled Dermo1+ mesenchymal cells	818:854	In experimental models of lung injury by lipopolysaccharide and naphthalene, GFP-labeled Dermo1+ mesenchymal cells were traced during injury repair.
31791401	1	8	theme	cell	171:174	arg1	types					176:180	highly specialized cell types	152:180	highly specialized cell types that are the target of airborne toxicants and injury	152:233	BACKGROUND The airways of mammalian lung are lined with highly specialized cell types that are the target of airborne toxicants and injury.
31791401	1	8	theme	cell	171:174	arg1	target					195:200	the target	191:200	the target of airborne toxicants and injury	191:233	BACKGROUND The airways of mammalian lung are lined with highly specialized cell types that are the target of airborne toxicants and injury.
31791401	12	9	theme	lung	2002:2005	arg1	repair					2007:2012	lung repair	2002:2012	lung repair	2002:2012	These findings have implications for understanding the regulation of lung repair and the potential for usage of mesenchymal stem cells in therapeutic strategies for lung diseases.
31791401	3	10	theme	epithelium	565:574	arg1	composition					543:553	the normal composition	532:553	the normal composition of airway epithelium following injury	532:591	However, the contributions of endogenous mesenchymal cells to recruitment, expansion or differentiation of stem cells, and repair and reestablishment of the normal composition of airway epithelium following injury have not been addressed.
31791401	6	11	theme	in	987:988	arg1	data					995:998	in vivo data	987:998	in vivo data	987:998	In vitro lung explant culture treated with or without lipopolysaccharide was also used to verify in vivo data.
31791401	2	12	link	marrow-derived	275:288	arg1	cells					351:355	stem cells	346:355	stem cells	346:355	Several epithelial cell types and bone marrow-derived mesenchymal stem cells have been identified to serve as stem cells during injury repair.
31791401	2	12	link	marrow-derived	275:288	arg1	types					260:264	Several epithelial cell types	236:264	Several epithelial cell types	236:264	Several epithelial cell types and bone marrow-derived mesenchymal stem cells have been identified to serve as stem cells during injury repair.
31791401	2	12	link	marrow-derived	275:288	arg1	cells					307:311	bone marrow-derived mesenchymal stem cells	270:311	bone marrow-derived mesenchymal stem cells	270:311	Several epithelial cell types and bone marrow-derived mesenchymal stem cells have been identified to serve as stem cells during injury repair.
31791401	2	13	theme	mesenchymal	290:300	arg1	cells					351:355	stem cells	346:355	stem cells	346:355	Several epithelial cell types and bone marrow-derived mesenchymal stem cells have been identified to serve as stem cells during injury repair.
31791401	2	13	theme	mesenchymal	290:300	arg1	types					260:264	Several epithelial cell types	236:264	Several epithelial cell types	236:264	Several epithelial cell types and bone marrow-derived mesenchymal stem cells have been identified to serve as stem cells during injury repair.
31791401	2	13	theme	mesenchymal	290:300	arg1	cells					307:311	bone marrow-derived mesenchymal stem cells	270:311	bone marrow-derived mesenchymal stem cells	270:311	Several epithelial cell types and bone marrow-derived mesenchymal stem cells have been identified to serve as stem cells during injury repair.
31791401	9	14	theme	epithelial	1486:1495	arg1	cells					1532:1536	neuroendocrine cells	1517:1536	neuroendocrine cells	1517:1536	The Dermo1+ stem cells was migrated into the airway epithelium layer sooner after injury, and sequentially differentiated transitionally to epithelial stem cells, such as neuroendocrine cells, and finally to newly differentiated Club cells, ciliated cells, and goblet cells in injury repair.
31791401	9	14	theme	epithelial	1486:1495	arg1	cells					1502:1506	epithelial stem cells	1486:1506	epithelial stem cells	1486:1506	The Dermo1+ stem cells was migrated into the airway epithelium layer sooner after injury, and sequentially differentiated transitionally to epithelial stem cells, such as neuroendocrine cells, and finally to newly differentiated Club cells, ciliated cells, and goblet cells in injury repair.
31791401	12	15	theme	lung	2098:2101	arg1	diseases					2103:2110	lung diseases	2098:2110	lung diseases	2098:2110	These findings have implications for understanding the regulation of lung repair and the potential for usage of mesenchymal stem cells in therapeutic strategies for lung diseases.
31791401	3	16	theme	mesenchymal	420:430	arg1	cells					432:436	endogenous mesenchymal cells	409:436	endogenous mesenchymal cells	409:436	However, the contributions of endogenous mesenchymal cells to recruitment, expansion or differentiation of stem cells, and repair and reestablishment of the normal composition of airway epithelium following injury have not been addressed.
31791401	4	17	theme	mouse	638:642	arg1	cells					666:670	mouse pulmonary mesenchymal cells	638:670	mouse pulmonary mesenchymal cells	638:670	METHODS The role of mouse pulmonary mesenchymal cells was investigated by lineage tracing using Dermo1-Cre; ROSAmTmG mice.
31791401	11	18	theme	mesenchymal	1821:1831	arg1	cell					1838:1841	The Dermo1+ mesenchymal stem cell	1809:1841	The Dermo1+ mesenchymal stem cell	1809:1841	The Dermo1+ mesenchymal stem cell differentiated into epithelial stem cells before reestablishing various epithelial cells.
31791401	6	19	used	used	972:975	arg2	culture					912:918	In vitro lung explant culture	890:918	In vitro lung explant culture treated with or without lipopolysaccharide	890:961	In vitro lung explant culture treated with or without lipopolysaccharide was also used to verify in vivo data.
31791401	5	20	theme	Dermo1+	830:836	arg1	cells					850:854	GFP-labeled Dermo1+ mesenchymal cells	818:854	GFP-labeled Dermo1+ mesenchymal cells	818:854	In experimental models of lung injury by lipopolysaccharide and naphthalene, GFP-labeled Dermo1+ mesenchymal cells were traced during injury repair.
31791401	11	21	theme	Dermo1+	1813:1819	arg1	cell					1838:1841	The Dermo1+ mesenchymal stem cell	1809:1841	The Dermo1+ mesenchymal stem cell	1809:1841	The Dermo1+ mesenchymal stem cell differentiated into epithelial stem cells before reestablishing various epithelial cells.
31791401	8	22	theme	Club	1223:1226	arg1	model					1261:1265	Club cell-specific naphthalene injury model	1223:1265	Club cell-specific naphthalene injury model	1223:1265	In Club cell-specific naphthalene injury model, the process of Dermo1+ stem cell regenerating epithelial cells was dissected.
31791401	4	23	theme	Dermo1-Cre	714:723	arg1	mice					735:738	Dermo1-Cre; ROSAmTmG mice	714:738	Dermo1-Cre; ROSAmTmG mice	714:738	METHODS The role of mouse pulmonary mesenchymal cells was investigated by lineage tracing using Dermo1-Cre; ROSAmTmG mice.
31791401	7	24	theme	Dermo1+	1057:1063	arg1	cells					1077:1081	GFP-labeled Dermo1+ mesenchymal cells	1045:1081	GFP-labeled Dermo1+ mesenchymal cells	1045:1081	RESULTS During injury repair, a subgroup of GFP-labeled Dermo1+ mesenchymal cells were found to contribute to normal repair of the airway epithelium and differentiated into Club cells, ciliated cells, and goblet cells.
31791401	4	25	dep	METHODS	618:624	arg1	role					630:633	The role	626:633	METHODS The role of mouse pulmonary mesenchymal cells	618:670	METHODS The role of mouse pulmonary mesenchymal cells was investigated by lineage tracing using Dermo1-Cre; ROSAmTmG mice.
31791401	7	26	theme	injury	1016:1021	arg1	repair					1023:1028	injury repair	1016:1028	injury repair	1016:1028	RESULTS During injury repair, a subgroup of GFP-labeled Dermo1+ mesenchymal cells were found to contribute to normal repair of the airway epithelium and differentiated into Club cells, ciliated cells, and goblet cells.
31791401	8	27	theme	regenerating	1301:1312	arg1	cells					1325:1329	Dermo1+ stem cell regenerating epithelial cells	1283:1329	Dermo1+ stem cell regenerating epithelial cells	1283:1329	In Club cell-specific naphthalene injury model, the process of Dermo1+ stem cell regenerating epithelial cells was dissected.
31791401	2	28	theme	epithelial	244:253	arg1	cells					307:311	bone marrow-derived mesenchymal stem cells	270:311	bone marrow-derived mesenchymal stem cells	270:311	Several epithelial cell types and bone marrow-derived mesenchymal stem cells have been identified to serve as stem cells during injury repair.
31791401	2	28	theme	epithelial	244:253	arg1	types					260:264	Several epithelial cell types	236:264	Several epithelial cell types	236:264	Several epithelial cell types and bone marrow-derived mesenchymal stem cells have been identified to serve as stem cells during injury repair.
31791401	2	28	theme	epithelial	244:253	arg1	cells					351:355	stem cells	346:355	stem cells	346:355	Several epithelial cell types and bone marrow-derived mesenchymal stem cells have been identified to serve as stem cells during injury repair.
31791401	9	29	theme	neuroendocrine	1517:1530	arg1	cells					1532:1536	neuroendocrine cells	1517:1536	neuroendocrine cells	1517:1536	The Dermo1+ stem cells was migrated into the airway epithelium layer sooner after injury, and sequentially differentiated transitionally to epithelial stem cells, such as neuroendocrine cells, and finally to newly differentiated Club cells, ciliated cells, and goblet cells in injury repair.
31791401	9	30	from	cells	1580:1584	arg1	repair					1630:1635	injury repair	1623:1635	injury repair	1623:1635	The Dermo1+ stem cells was migrated into the airway epithelium layer sooner after injury, and sequentially differentiated transitionally to epithelial stem cells, such as neuroendocrine cells, and finally to newly differentiated Club cells, ciliated cells, and goblet cells in injury repair.
31791401	4	31	theme	ROSAmTmG	726:733	arg1	mice					735:738	Dermo1-Cre; ROSAmTmG mice	714:738	Dermo1-Cre; ROSAmTmG mice	714:738	METHODS The role of mouse pulmonary mesenchymal cells was investigated by lineage tracing using Dermo1-Cre; ROSAmTmG mice.
31791401	6	32	dep	in	987:988	arg1	vivo					990:993	vivo	990:993	vivo	990:993	In vitro lung explant culture treated with or without lipopolysaccharide was also used to verify in vivo data.
31791401	8	33	theme	cells	1325:1329	arg1	process					1272:1278	the process	1268:1278	the process of Dermo1+ stem cell regenerating epithelial cells	1268:1329	In Club cell-specific naphthalene injury model, the process of Dermo1+ stem cell regenerating epithelial cells was dissected.
31791401	9	34	from	cells	1596:1600	arg1	repair					1630:1635	injury repair	1623:1635	injury repair	1623:1635	The Dermo1+ stem cells was migrated into the airway epithelium layer sooner after injury, and sequentially differentiated transitionally to epithelial stem cells, such as neuroendocrine cells, and finally to newly differentiated Club cells, ciliated cells, and goblet cells in injury repair.
31791401	5	35	theme	injury	772:777	arg1	models					757:762	experimental models	744:762	experimental models of lung injury by lipopolysaccharide and naphthalene	744:815	In experimental models of lung injury by lipopolysaccharide and naphthalene, GFP-labeled Dermo1+ mesenchymal cells were traced during injury repair.
31791401	7	36	theme	goblet	1206:1211	arg1	cells					1213:1217	goblet cells	1206:1217	goblet cells	1206:1217	RESULTS During injury repair, a subgroup of GFP-labeled Dermo1+ mesenchymal cells were found to contribute to normal repair of the airway epithelium and differentiated into Club cells, ciliated cells, and goblet cells.
31791401	11	37	theme	epithelial	1915:1924	arg1	cells					1926:1930	various epithelial cells	1907:1930	various epithelial cells	1907:1930	The Dermo1+ mesenchymal stem cell differentiated into epithelial stem cells before reestablishing various epithelial cells.
31791401	1	38	theme	lung	132:135	arg1	airways					111:117	The airways	107:117	The airways of mammalian lung	107:135	BACKGROUND The airways of mammalian lung are lined with highly specialized cell types that are the target of airborne toxicants and injury.
31791401	3	39	theme	cells	432:436	arg1	contributions					392:404	the contributions	388:404	the contributions of endogenous mesenchymal cells to recruitment, expansion or differentiation of stem cells, and repair and reestablishment of the normal composition of airway epithelium following injury	388:591	However, the contributions of endogenous mesenchymal cells to recruitment, expansion or differentiation of stem cells, and repair and reestablishment of the normal composition of airway epithelium following injury have not been addressed.
31791401	5	40	theme	experimental	744:755	arg1	models					757:762	experimental models	744:762	experimental models of lung injury by lipopolysaccharide and naphthalene	744:815	In experimental models of lung injury by lipopolysaccharide and naphthalene, GFP-labeled Dermo1+ mesenchymal cells were traced during injury repair.
31791401	9	41	theme	Club	1575:1578	arg1	cells					1580:1584	newly differentiated Club cells	1554:1584	newly differentiated Club cells	1554:1584	The Dermo1+ stem cells was migrated into the airway epithelium layer sooner after injury, and sequentially differentiated transitionally to epithelial stem cells, such as neuroendocrine cells, and finally to newly differentiated Club cells, ciliated cells, and goblet cells in injury repair.
31791401	6	42	theme	explant	904:910	arg1	culture					912:918	In vitro lung explant culture	890:918	In vitro lung explant culture treated with or without lipopolysaccharide	890:961	In vitro lung explant culture treated with or without lipopolysaccharide was also used to verify in vivo data.
31791401	3	43	theme	endogenous	409:418	arg1	cells					432:436	endogenous mesenchymal cells	409:436	endogenous mesenchymal cells	409:436	However, the contributions of endogenous mesenchymal cells to recruitment, expansion or differentiation of stem cells, and repair and reestablishment of the normal composition of airway epithelium following injury have not been addressed.
31791401	7	44	theme	ciliated	1186:1193	arg1	cells					1195:1199	ciliated cells	1186:1199	ciliated cells	1186:1199	RESULTS During injury repair, a subgroup of GFP-labeled Dermo1+ mesenchymal cells were found to contribute to normal repair of the airway epithelium and differentiated into Club cells, ciliated cells, and goblet cells.
31791401	12	45	contain	have	1948:1951	arg2	implications					1953:1964	implications	1953:1964	implications for understanding the regulation of lung repair and the potential for usage of mesenchymal stem cells in therapeutic strategies for lung diseases	1953:2110	These findings have implications for understanding the regulation of lung repair and the potential for usage of mesenchymal stem cells in therapeutic strategies for lung diseases.
31791401	12	45	contain	have	1948:1951	arg1	findings					1939:1946	These findings	1933:1946	These findings	1933:1946	These findings have implications for understanding the regulation of lung repair and the potential for usage of mesenchymal stem cells in therapeutic strategies for lung diseases.
31791401	5	46	theme	mesenchymal	838:848	arg1	cells					850:854	GFP-labeled Dermo1+ mesenchymal cells	818:854	GFP-labeled Dermo1+ mesenchymal cells	818:854	In experimental models of lung injury by lipopolysaccharide and naphthalene, GFP-labeled Dermo1+ mesenchymal cells were traced during injury repair.
31791401	9	47	theme	Dermo1+	1350:1356	arg1	cells					1363:1367	The Dermo1+ stem cells	1346:1367	The Dermo1+ stem cells	1346:1367	The Dermo1+ stem cells was migrated into the airway epithelium layer sooner after injury, and sequentially differentiated transitionally to epithelial stem cells, such as neuroendocrine cells, and finally to newly differentiated Club cells, ciliated cells, and goblet cells in injury repair.
31791401	6	48	theme	In	890:891	arg1	culture					912:918	In vitro lung explant culture	890:918	In vitro lung explant culture treated with or without lipopolysaccharide	890:961	In vitro lung explant culture treated with or without lipopolysaccharide was also used to verify in vivo data.
31791401	1	49	dep	BACKGROUND	96:105	arg1	lined					141:145	lined	141:145	are lined with highly specialized cell types that are the target of airborne toxicants and injury	137:233	BACKGROUND The airways of mammalian lung are lined with highly specialized cell types that are the target of airborne toxicants and injury.
31791401	10	50	theme	epithelial	1758:1767	arg1	regeneration					1774:1785	airway epithelial cell regeneration	1751:1785	airway epithelial cell regeneration	1751:1785	CONCLUSION In this study, a population of Dermo1+ mesenchymal stem cell was identified to serve as stem cells in airway epithelial cell regeneration during injury repair.
31791401	1	51	theme	specialized	159:169	arg1	types					176:180	highly specialized cell types	152:180	highly specialized cell types that are the target of airborne toxicants and injury	152:233	BACKGROUND The airways of mammalian lung are lined with highly specialized cell types that are the target of airborne toxicants and injury.
31791401	1	51	theme	specialized	159:169	arg1	target					195:200	the target	191:200	the target of airborne toxicants and injury	191:233	BACKGROUND The airways of mammalian lung are lined with highly specialized cell types that are the target of airborne toxicants and injury.
31791401	0	52	theme	mesenchymal	22:32	arg1	cells					34:38	pulmonary mesenchymal cells	12:38	pulmonary mesenchymal cells	12:38	The role of pulmonary mesenchymal cells in airway epithelium regeneration during injury repair.
31791401	3	53	theme	stem	486:489	arg1	cells					491:495	stem cells	486:495	stem cells	486:495	However, the contributions of endogenous mesenchymal cells to recruitment, expansion or differentiation of stem cells, and repair and reestablishment of the normal composition of airway epithelium following injury have not been addressed.
31791401	11	54	theme	stem	1874:1877	arg1	cells					1879:1883	epithelial stem cells	1863:1883	epithelial stem cells	1863:1883	The Dermo1+ mesenchymal stem cell differentiated into epithelial stem cells before reestablishing various epithelial cells.
31791401	10	55	theme	mesenchymal	1688:1698	arg1	cell					1705:1708	Dermo1+ mesenchymal stem cell	1680:1708	Dermo1+ mesenchymal stem cell	1680:1708	CONCLUSION In this study, a population of Dermo1+ mesenchymal stem cell was identified to serve as stem cells in airway epithelial cell regeneration during injury repair.
31791401	1	56	theme	airborne	205:212	arg1	toxicants					214:222	airborne toxicants	205:222	airborne toxicants	205:222	BACKGROUND The airways of mammalian lung are lined with highly specialized cell types that are the target of airborne toxicants and injury.
31791401	9	57	theme	injury	1623:1628	arg1	repair					1630:1635	injury repair	1623:1635	injury repair	1623:1635	The Dermo1+ stem cells was migrated into the airway epithelium layer sooner after injury, and sequentially differentiated transitionally to epithelial stem cells, such as neuroendocrine cells, and finally to newly differentiated Club cells, ciliated cells, and goblet cells in injury repair.
31791401	4	58	theme	pulmonary	644:652	arg1	cells					666:670	mouse pulmonary mesenchymal cells	638:670	mouse pulmonary mesenchymal cells	638:670	METHODS The role of mouse pulmonary mesenchymal cells was investigated by lineage tracing using Dermo1-Cre; ROSAmTmG mice.
31791401	9	59	theme	epithelium	1398:1407	arg1	layer					1409:1413	the airway epithelium layer	1387:1413	the airway epithelium layer	1387:1413	The Dermo1+ stem cells was migrated into the airway epithelium layer sooner after injury, and sequentially differentiated transitionally to epithelial stem cells, such as neuroendocrine cells, and finally to newly differentiated Club cells, ciliated cells, and goblet cells in injury repair.
31791401	7	60	theme	epithelium	1139:1148	arg1	repair					1118:1123	normal repair	1111:1123	normal repair of the airway epithelium	1111:1148	RESULTS During injury repair, a subgroup of GFP-labeled Dermo1+ mesenchymal cells were found to contribute to normal repair of the airway epithelium and differentiated into Club cells, ciliated cells, and goblet cells.
31791401	5	61	theme	lung	767:770	arg1	injury					772:777	lung injury	767:777	lung injury	767:777	In experimental models of lung injury by lipopolysaccharide and naphthalene, GFP-labeled Dermo1+ mesenchymal cells were traced during injury repair.
31791401	2	62	theme	stem	346:349	arg1	cells					307:311	bone marrow-derived mesenchymal stem cells	270:311	bone marrow-derived mesenchymal stem cells	270:311	Several epithelial cell types and bone marrow-derived mesenchymal stem cells have been identified to serve as stem cells during injury repair.
31791401	2	62	theme	stem	346:349	arg1	types					260:264	Several epithelial cell types	236:264	Several epithelial cell types	236:264	Several epithelial cell types and bone marrow-derived mesenchymal stem cells have been identified to serve as stem cells during injury repair.
31791401	2	62	theme	stem	346:349	arg1	cells					351:355	stem cells	346:355	stem cells	346:355	Several epithelial cell types and bone marrow-derived mesenchymal stem cells have been identified to serve as stem cells during injury repair.
31791401	12	63	theme	stem	2057:2060	arg1	cells					2062:2066	mesenchymal stem cells	2045:2066	mesenchymal stem cells	2045:2066	These findings have implications for understanding the regulation of lung repair and the potential for usage of mesenchymal stem cells in therapeutic strategies for lung diseases.
31791401	2	64	theme	marrow-derived	275:288	arg1	cells					351:355	stem cells	346:355	stem cells	346:355	Several epithelial cell types and bone marrow-derived mesenchymal stem cells have been identified to serve as stem cells during injury repair.
31791401	2	64	theme	marrow-derived	275:288	arg1	types					260:264	Several epithelial cell types	236:264	Several epithelial cell types	236:264	Several epithelial cell types and bone marrow-derived mesenchymal stem cells have been identified to serve as stem cells during injury repair.
31791401	2	64	theme	marrow-derived	275:288	arg1	cells					307:311	bone marrow-derived mesenchymal stem cells	270:311	bone marrow-derived mesenchymal stem cells	270:311	Several epithelial cell types and bone marrow-derived mesenchymal stem cells have been identified to serve as stem cells during injury repair.
31791401	12	65	from	usage	2036:2040	arg1	strategies					2083:2092	therapeutic strategies	2071:2092	therapeutic strategies for lung diseases	2071:2110	These findings have implications for understanding the regulation of lung repair and the potential for usage of mesenchymal stem cells in therapeutic strategies for lung diseases.
31791401	10	66	theme	cell	1705:1708	arg1	cells					1742:1746	stem cells	1737:1746	stem cells in airway epithelial cell regeneration	1737:1785	CONCLUSION In this study, a population of Dermo1+ mesenchymal stem cell was identified to serve as stem cells in airway epithelial cell regeneration during injury repair.
31791401	10	66	theme	cell	1705:1708	arg1	population					1666:1675	a population	1664:1675	a population of Dermo1+ mesenchymal stem cell	1664:1708	CONCLUSION In this study, a population of Dermo1+ mesenchymal stem cell was identified to serve as stem cells in airway epithelial cell regeneration during injury repair.
31791401	10	67	theme	stem	1737:1740	arg1	cells					1742:1746	stem cells	1737:1746	stem cells in airway epithelial cell regeneration	1737:1785	CONCLUSION In this study, a population of Dermo1+ mesenchymal stem cell was identified to serve as stem cells in airway epithelial cell regeneration during injury repair.
31791401	10	67	theme	stem	1737:1740	arg1	population					1666:1675	a population	1664:1675	a population of Dermo1+ mesenchymal stem cell	1664:1708	CONCLUSION In this study, a population of Dermo1+ mesenchymal stem cell was identified to serve as stem cells in airway epithelial cell regeneration during injury repair.
31791401	5	68	theme	injury	875:880	arg1	repair					882:887	injury repair	875:887	injury repair	875:887	In experimental models of lung injury by lipopolysaccharide and naphthalene, GFP-labeled Dermo1+ mesenchymal cells were traced during injury repair.
31791401	6	69	theme	lung	899:902	arg1	culture					912:918	In vitro lung explant culture	890:918	In vitro lung explant culture treated with or without lipopolysaccharide	890:961	In vitro lung explant culture treated with or without lipopolysaccharide was also used to verify in vivo data.
31791401	8	70	theme	cell-specific	1228:1240	arg1	model					1261:1265	Club cell-specific naphthalene injury model	1223:1265	Club cell-specific naphthalene injury model	1223:1265	In Club cell-specific naphthalene injury model, the process of Dermo1+ stem cell regenerating epithelial cells was dissected.
31791401	12	71	theme	therapeutic	2071:2081	arg1	strategies					2083:2092	therapeutic strategies	2071:2092	therapeutic strategies for lung diseases	2071:2110	These findings have implications for understanding the regulation of lung repair and the potential for usage of mesenchymal stem cells in therapeutic strategies for lung diseases.
31791401	3	72	theme	airway	558:563	arg1	epithelium					565:574	airway epithelium	558:574	airway epithelium	558:574	However, the contributions of endogenous mesenchymal cells to recruitment, expansion or differentiation of stem cells, and repair and reestablishment of the normal composition of airway epithelium following injury have not been addressed.
31791401	8	73	theme	injury	1254:1259	arg1	model					1261:1265	Club cell-specific naphthalene injury model	1223:1265	Club cell-specific naphthalene injury model	1223:1265	In Club cell-specific naphthalene injury model, the process of Dermo1+ stem cell regenerating epithelial cells was dissected.
31791401	2	74	theme	stem	302:305	arg1	cells					351:355	stem cells	346:355	stem cells	346:355	Several epithelial cell types and bone marrow-derived mesenchymal stem cells have been identified to serve as stem cells during injury repair.
31791401	2	74	theme	stem	302:305	arg1	types					260:264	Several epithelial cell types	236:264	Several epithelial cell types	236:264	Several epithelial cell types and bone marrow-derived mesenchymal stem cells have been identified to serve as stem cells during injury repair.
31791401	2	74	theme	stem	302:305	arg1	cells					307:311	bone marrow-derived mesenchymal stem cells	270:311	bone marrow-derived mesenchymal stem cells	270:311	Several epithelial cell types and bone marrow-derived mesenchymal stem cells have been identified to serve as stem cells during injury repair.
31791401	10	75	theme	cell	1769:1772	arg1	regeneration					1774:1785	airway epithelial cell regeneration	1751:1785	airway epithelial cell regeneration	1751:1785	CONCLUSION In this study, a population of Dermo1+ mesenchymal stem cell was identified to serve as stem cells in airway epithelial cell regeneration during injury repair.
31791401	10	76	theme	stem	1700:1703	arg1	cell					1705:1708	Dermo1+ mesenchymal stem cell	1680:1708	Dermo1+ mesenchymal stem cell	1680:1708	CONCLUSION In this study, a population of Dermo1+ mesenchymal stem cell was identified to serve as stem cells in airway epithelial cell regeneration during injury repair.
31791401	7	77	theme	mesenchymal	1065:1075	arg1	cells					1077:1081	GFP-labeled Dermo1+ mesenchymal cells	1045:1081	GFP-labeled Dermo1+ mesenchymal cells	1045:1081	RESULTS During injury repair, a subgroup of GFP-labeled Dermo1+ mesenchymal cells were found to contribute to normal repair of the airway epithelium and differentiated into Club cells, ciliated cells, and goblet cells.
31791401	4	78	theme	cells	666:670	arg1	role					630:633	The role	626:633	METHODS The role of mouse pulmonary mesenchymal cells	618:670	METHODS The role of mouse pulmonary mesenchymal cells was investigated by lineage tracing using Dermo1-Cre; ROSAmTmG mice.
31791401	9	79	theme	stem	1497:1500	arg1	cells					1532:1536	neuroendocrine cells	1517:1536	neuroendocrine cells	1517:1536	The Dermo1+ stem cells was migrated into the airway epithelium layer sooner after injury, and sequentially differentiated transitionally to epithelial stem cells, such as neuroendocrine cells, and finally to newly differentiated Club cells, ciliated cells, and goblet cells in injury repair.
31791401	9	79	theme	stem	1497:1500	arg1	cells					1502:1506	epithelial stem cells	1486:1506	epithelial stem cells	1486:1506	The Dermo1+ stem cells was migrated into the airway epithelium layer sooner after injury, and sequentially differentiated transitionally to epithelial stem cells, such as neuroendocrine cells, and finally to newly differentiated Club cells, ciliated cells, and goblet cells in injury repair.
31791401	11	80	theme	stem	1833:1836	arg1	cell					1838:1841	The Dermo1+ mesenchymal stem cell	1809:1841	The Dermo1+ mesenchymal stem cell	1809:1841	The Dermo1+ mesenchymal stem cell differentiated into epithelial stem cells before reestablishing various epithelial cells.
31791401	7	81	theme	GFP-labeled	1045:1055	arg1	cells					1077:1081	GFP-labeled Dermo1+ mesenchymal cells	1045:1081	GFP-labeled Dermo1+ mesenchymal cells	1045:1081	RESULTS During injury repair, a subgroup of GFP-labeled Dermo1+ mesenchymal cells were found to contribute to normal repair of the airway epithelium and differentiated into Club cells, ciliated cells, and goblet cells.
31791401	10	82	theme	injury	1794:1799	arg1	repair					1801:1806	injury repair	1794:1806	injury repair	1794:1806	CONCLUSION In this study, a population of Dermo1+ mesenchymal stem cell was identified to serve as stem cells in airway epithelial cell regeneration during injury repair.
31791401	8	83	theme	Dermo1+	1283:1289	arg1	cells					1325:1329	Dermo1+ stem cell regenerating epithelial cells	1283:1329	Dermo1+ stem cell regenerating epithelial cells	1283:1329	In Club cell-specific naphthalene injury model, the process of Dermo1+ stem cell regenerating epithelial cells was dissected.
31791401	7	84	theme	Club	1174:1177	arg1	cells					1179:1183	Club cells	1174:1183	Club cells	1174:1183	RESULTS During injury repair, a subgroup of GFP-labeled Dermo1+ mesenchymal cells were found to contribute to normal repair of the airway epithelium and differentiated into Club cells, ciliated cells, and goblet cells.
31791401	2	85	theme	cell	255:258	arg1	cells					307:311	bone marrow-derived mesenchymal stem cells	270:311	bone marrow-derived mesenchymal stem cells	270:311	Several epithelial cell types and bone marrow-derived mesenchymal stem cells have been identified to serve as stem cells during injury repair.
31791401	2	85	theme	cell	255:258	arg1	types					260:264	Several epithelial cell types	236:264	Several epithelial cell types	236:264	Several epithelial cell types and bone marrow-derived mesenchymal stem cells have been identified to serve as stem cells during injury repair.
31791401	2	85	theme	cell	255:258	arg1	cells					351:355	stem cells	346:355	stem cells	346:355	Several epithelial cell types and bone marrow-derived mesenchymal stem cells have been identified to serve as stem cells during injury repair.
31791401	1	86	theme	mammalian	122:130	arg1	lung					132:135	mammalian lung	122:135	mammalian lung	122:135	BACKGROUND The airways of mammalian lung are lined with highly specialized cell types that are the target of airborne toxicants and injury.
31791401	3	87	theme	composition	543:553	arg1	repair					502:507	repair	502:507	repair	502:507	However, the contributions of endogenous mesenchymal cells to recruitment, expansion or differentiation of stem cells, and repair and reestablishment of the normal composition of airway epithelium following injury have not been addressed.
31791401	3	87	theme	composition	543:553	arg1	differentiation					467:481	differentiation	467:481	differentiation	467:481	However, the contributions of endogenous mesenchymal cells to recruitment, expansion or differentiation of stem cells, and repair and reestablishment of the normal composition of airway epithelium following injury have not been addressed.
31791401	3	87	theme	composition	543:553	arg1	expansion					454:462	expansion	454:462	expansion	454:462	However, the contributions of endogenous mesenchymal cells to recruitment, expansion or differentiation of stem cells, and repair and reestablishment of the normal composition of airway epithelium following injury have not been addressed.
31791401	3	87	theme	composition	543:553	arg1	reestablishment					513:527	reestablishment	513:527	reestablishment	513:527	However, the contributions of endogenous mesenchymal cells to recruitment, expansion or differentiation of stem cells, and repair and reestablishment of the normal composition of airway epithelium following injury have not been addressed.
31791401	3	87	theme	composition	543:553	arg1	recruitment					441:451	recruitment	441:451	recruitment	441:451	However, the contributions of endogenous mesenchymal cells to recruitment, expansion or differentiation of stem cells, and repair and reestablishment of the normal composition of airway epithelium following injury have not been addressed.
31791401	8	88	theme	cell	1296:1299	arg1	cells					1325:1329	Dermo1+ stem cell regenerating epithelial cells	1283:1329	Dermo1+ stem cell regenerating epithelial cells	1283:1329	In Club cell-specific naphthalene injury model, the process of Dermo1+ stem cell regenerating epithelial cells was dissected.
31791401	2	89	theme	Several	236:242	arg1	cells					307:311	bone marrow-derived mesenchymal stem cells	270:311	bone marrow-derived mesenchymal stem cells	270:311	Several epithelial cell types and bone marrow-derived mesenchymal stem cells have been identified to serve as stem cells during injury repair.
31791401	2	89	theme	Several	236:242	arg1	types					260:264	Several epithelial cell types	236:264	Several epithelial cell types	236:264	Several epithelial cell types and bone marrow-derived mesenchymal stem cells have been identified to serve as stem cells during injury repair.
31791401	2	89	theme	Several	236:242	arg1	cells					351:355	stem cells	346:355	stem cells	346:355	Several epithelial cell types and bone marrow-derived mesenchymal stem cells have been identified to serve as stem cells during injury repair.
31791401	10	90	from	cells	1742:1746	arg1	regeneration					1774:1785	airway epithelial cell regeneration	1751:1785	airway epithelial cell regeneration	1751:1785	CONCLUSION In this study, a population of Dermo1+ mesenchymal stem cell was identified to serve as stem cells in airway epithelial cell regeneration during injury repair.
31791401	12	91	theme	repair	2007:2012	arg1	regulation					1988:1997	the regulation	1984:1997	the regulation of lung repair	1984:2012	These findings have implications for understanding the regulation of lung repair and the potential for usage of mesenchymal stem cells in therapeutic strategies for lung diseases.
31791401	12	91	theme	repair	2007:2012	arg1	potential					2022:2030	the potential	2018:2030	the potential for usage of mesenchymal stem cells in therapeutic strategies for lung diseases	2018:2110	These findings have implications for understanding the regulation of lung repair and the potential for usage of mesenchymal stem cells in therapeutic strategies for lung diseases.
31791401	8	92	theme	epithelial	1314:1323	arg1	cells					1325:1329	Dermo1+ stem cell regenerating epithelial cells	1283:1329	Dermo1+ stem cell regenerating epithelial cells	1283:1329	In Club cell-specific naphthalene injury model, the process of Dermo1+ stem cell regenerating epithelial cells was dissected.
31791401	0	93	theme	injury	81:86	arg1	repair					88:93	injury repair	81:93	injury repair	81:93	The role of pulmonary mesenchymal cells in airway epithelium regeneration during injury repair.
31791401	0	94	from	role	4:7	arg1	epithelium					50:59	airway epithelium	43:59	airway epithelium	43:59	The role of pulmonary mesenchymal cells in airway epithelium regeneration during injury repair.
31791401	4	95	theme	lineage	692:698	arg1	tracing					700:706	lineage tracing	692:706	lineage tracing	692:706	METHODS The role of mouse pulmonary mesenchymal cells was investigated by lineage tracing using Dermo1-Cre; ROSAmTmG mice.
31791401	10	96	theme	airway	1751:1756	arg1	regeneration					1774:1785	airway epithelial cell regeneration	1751:1785	airway epithelial cell regeneration	1751:1785	CONCLUSION In this study, a population of Dermo1+ mesenchymal stem cell was identified to serve as stem cells in airway epithelial cell regeneration during injury repair.
31791401	9	97	theme	differentiated	1560:1573	arg1	cells					1580:1584	newly differentiated Club cells	1554:1584	newly differentiated Club cells	1554:1584	The Dermo1+ stem cells was migrated into the airway epithelium layer sooner after injury, and sequentially differentiated transitionally to epithelial stem cells, such as neuroendocrine cells, and finally to newly differentiated Club cells, ciliated cells, and goblet cells in injury repair.
31791401	1	98	theme	toxicants	214:222	arg1	types					176:180	highly specialized cell types	152:180	highly specialized cell types that are the target of airborne toxicants and injury	152:233	BACKGROUND The airways of mammalian lung are lined with highly specialized cell types that are the target of airborne toxicants and injury.
31791401	1	98	theme	toxicants	214:222	arg1	target					195:200	the target	191:200	the target of airborne toxicants and injury	191:233	BACKGROUND The airways of mammalian lung are lined with highly specialized cell types that are the target of airborne toxicants and injury.
31791401	8	99	theme	naphthalene	1242:1252	arg1	model					1261:1265	Club cell-specific naphthalene injury model	1223:1265	Club cell-specific naphthalene injury model	1223:1265	In Club cell-specific naphthalene injury model, the process of Dermo1+ stem cell regenerating epithelial cells was dissected.
31791401	2	100	theme	injury	364:369	arg1	repair					371:376	injury repair	364:376	injury repair	364:376	Several epithelial cell types and bone marrow-derived mesenchymal stem cells have been identified to serve as stem cells during injury repair.
31791401	0	101	theme	pulmonary	12:20	arg1	cells					34:38	pulmonary mesenchymal cells	12:38	pulmonary mesenchymal cells	12:38	The role of pulmonary mesenchymal cells in airway epithelium regeneration during injury repair.
31791401	9	102	theme	ciliated	1587:1594	arg1	cells					1596:1600	ciliated cells	1587:1600	ciliated cells	1587:1600	The Dermo1+ stem cells was migrated into the airway epithelium layer sooner after injury, and sequentially differentiated transitionally to epithelial stem cells, such as neuroendocrine cells, and finally to newly differentiated Club cells, ciliated cells, and goblet cells in injury repair.
31791401	9	103	from	cells	1614:1618	arg1	repair					1630:1635	injury repair	1623:1635	injury repair	1623:1635	The Dermo1+ stem cells was migrated into the airway epithelium layer sooner after injury, and sequentially differentiated transitionally to epithelial stem cells, such as neuroendocrine cells, and finally to newly differentiated Club cells, ciliated cells, and goblet cells in injury repair.
31791401	7	104	dep	RESULTS	1001:1007	arg1	differentiated					1154:1167	differentiated	1154:1167	differentiated into Club cells, ciliated cells, and goblet cells	1154:1217	RESULTS During injury repair, a subgroup of GFP-labeled Dermo1+ mesenchymal cells were found to contribute to normal repair of the airway epithelium and differentiated into Club cells, ciliated cells, and goblet cells.
31791401	7	104	dep	RESULTS	1001:1007	arg1	found					1088:1092	found	1088:1092	found to contribute to normal repair of the airway epithelium	1088:1148	RESULTS During injury repair, a subgroup of GFP-labeled Dermo1+ mesenchymal cells were found to contribute to normal repair of the airway epithelium and differentiated into Club cells, ciliated cells, and goblet cells.
31791401	0	105	theme	cells	34:38	arg1	role					4:7	The role	0:7	The role of pulmonary mesenchymal cells in airway epithelium	0:59	The role of pulmonary mesenchymal cells in airway epithelium regeneration during injury repair.
31791401	9	106	theme	stem	1358:1361	arg1	cells					1363:1367	The Dermo1+ stem cells	1346:1367	The Dermo1+ stem cells	1346:1367	The Dermo1+ stem cells was migrated into the airway epithelium layer sooner after injury, and sequentially differentiated transitionally to epithelial stem cells, such as neuroendocrine cells, and finally to newly differentiated Club cells, ciliated cells, and goblet cells in injury repair.
31791401	0	107	theme	airway	43:48	arg1	epithelium					50:59	airway epithelium	43:59	airway epithelium	43:59	The role of pulmonary mesenchymal cells in airway epithelium regeneration during injury repair.
31791401	9	108	theme	goblet	1607:1612	arg1	cells					1614:1618	goblet cells	1607:1618	goblet cells in injury repair	1607:1635	The Dermo1+ stem cells was migrated into the airway epithelium layer sooner after injury, and sequentially differentiated transitionally to epithelial stem cells, such as neuroendocrine cells, and finally to newly differentiated Club cells, ciliated cells, and goblet cells in injury repair.
31791401	7	109	theme	cells	1077:1081	arg1	subgroup					1033:1040	a subgroup	1031:1040	a subgroup of GFP-labeled Dermo1+ mesenchymal cells	1031:1081	RESULTS During injury repair, a subgroup of GFP-labeled Dermo1+ mesenchymal cells were found to contribute to normal repair of the airway epithelium and differentiated into Club cells, ciliated cells, and goblet cells.
31791401	6	110	dep	In	890:891	arg1	vitro					893:897	vitro	893:897	vitro	893:897	In vitro lung explant culture treated with or without lipopolysaccharide was also used to verify in vivo data.
31791401	3	111	theme	cells	491:495	arg1	repair					502:507	repair	502:507	repair	502:507	However, the contributions of endogenous mesenchymal cells to recruitment, expansion or differentiation of stem cells, and repair and reestablishment of the normal composition of airway epithelium following injury have not been addressed.
31791401	3	111	theme	cells	491:495	arg1	differentiation					467:481	differentiation	467:481	differentiation	467:481	However, the contributions of endogenous mesenchymal cells to recruitment, expansion or differentiation of stem cells, and repair and reestablishment of the normal composition of airway epithelium following injury have not been addressed.
31791401	3	111	theme	cells	491:495	arg1	expansion					454:462	expansion	454:462	expansion	454:462	However, the contributions of endogenous mesenchymal cells to recruitment, expansion or differentiation of stem cells, and repair and reestablishment of the normal composition of airway epithelium following injury have not been addressed.
31791401	3	111	theme	cells	491:495	arg1	reestablishment					513:527	reestablishment	513:527	reestablishment	513:527	However, the contributions of endogenous mesenchymal cells to recruitment, expansion or differentiation of stem cells, and repair and reestablishment of the normal composition of airway epithelium following injury have not been addressed.
31791401	3	111	theme	cells	491:495	arg1	recruitment					441:451	recruitment	441:451	recruitment	441:451	However, the contributions of endogenous mesenchymal cells to recruitment, expansion or differentiation of stem cells, and repair and reestablishment of the normal composition of airway epithelium following injury have not been addressed.
31791401	11	112	theme	epithelial	1863:1872	arg1	cells					1879:1883	epithelial stem cells	1863:1883	epithelial stem cells	1863:1883	The Dermo1+ mesenchymal stem cell differentiated into epithelial stem cells before reestablishing various epithelial cells.
31791401	10	113	dep	CONCLUSION	1638:1647	arg1	study					1657:1661	this study	1652:1661	this study	1652:1661	CONCLUSION In this study, a population of Dermo1+ mesenchymal stem cell was identified to serve as stem cells in airway epithelial cell regeneration during injury repair.
31791401	10	114	theme	Dermo1+	1680:1686	arg1	cell					1705:1708	Dermo1+ mesenchymal stem cell	1680:1708	Dermo1+ mesenchymal stem cell	1680:1708	CONCLUSION In this study, a population of Dermo1+ mesenchymal stem cell was identified to serve as stem cells in airway epithelial cell regeneration during injury repair.
31791401	9	115	theme	airway	1391:1396	arg1	layer					1409:1413	the airway epithelium layer	1387:1413	the airway epithelium layer	1387:1413	The Dermo1+ stem cells was migrated into the airway epithelium layer sooner after injury, and sequentially differentiated transitionally to epithelial stem cells, such as neuroendocrine cells, and finally to newly differentiated Club cells, ciliated cells, and goblet cells in injury repair.
31791401	12	116	theme	mesenchymal	2045:2055	arg1	cells					2062:2066	mesenchymal stem cells	2045:2066	mesenchymal stem cells	2045:2066	These findings have implications for understanding the regulation of lung repair and the potential for usage of mesenchymal stem cells in therapeutic strategies for lung diseases.
31791401	1	117	theme	injury	228:233	arg1	types					176:180	highly specialized cell types	152:180	highly specialized cell types that are the target of airborne toxicants and injury	152:233	BACKGROUND The airways of mammalian lung are lined with highly specialized cell types that are the target of airborne toxicants and injury.
31791401	1	117	theme	injury	228:233	arg1	target					195:200	the target	191:200	the target of airborne toxicants and injury	191:233	BACKGROUND The airways of mammalian lung are lined with highly specialized cell types that are the target of airborne toxicants and injury.
31368546	10	0	theme	traditional	1279:1289	arg1	medicine					1291:1298	traditional medicine	1279:1298	traditional medicine since ancient times	1279:1318	PRACTICAL APPLICATIONS: Propolis is a resinous substance produced by the honeybee that has been adopted as a form of traditional medicine since ancient times.
31368546	10	1	theme	resinous	1200:1207	arg1	Propolis					1186:1193	Propolis	1186:1193	Propolis	1186:1193	PRACTICAL APPLICATIONS: Propolis is a resinous substance produced by the honeybee that has been adopted as a form of traditional medicine since ancient times.
31368546	10	1	theme	resinous	1200:1207	arg1	substance					1209:1217	a resinous substance	1198:1217	a resinous substance produced by the honeybee that has been adopted as a form of traditional medicine since ancient times	1198:1318	PRACTICAL APPLICATIONS: Propolis is a resinous substance produced by the honeybee that has been adopted as a form of traditional medicine since ancient times.
31368546	0	2	from	macrophages	186:196	arg1	downregulation					121:134	downregulation	121:134	downregulation of IL-1β and IL-6 expressions in murine RAW 264.7 macrophages	121:196	Propolis attenuates lipopolysaccharide-induced inflammatory responses through intracellular ROS and NO levels along with downregulation of IL-1β and IL-6 expressions in murine RAW 264.7 macrophages.
31368546	13	3	from	rich	1666:1669	arg1	flavonoids					1674:1683	flavonoids	1674:1683	flavonoids	1674:1683	This extraordinary substance is rich in flavonoids and antioxidants.
31368546	13	3	from	rich	1666:1669	arg1	antioxidants					1689:1700	antioxidants	1689:1700	antioxidants	1689:1700	This extraordinary substance is rich in flavonoids and antioxidants.
31368546	10	4	dep	APPLICATIONS	1172:1183	arg1	Propolis					1186:1193	Propolis	1186:1193	Propolis	1186:1193	PRACTICAL APPLICATIONS: Propolis is a resinous substance produced by the honeybee that has been adopted as a form of traditional medicine since ancient times.
31368546	10	4	dep	APPLICATIONS	1172:1183	arg1	substance					1209:1217	a resinous substance	1198:1217	a resinous substance produced by the honeybee that has been adopted as a form of traditional medicine since ancient times	1198:1318	PRACTICAL APPLICATIONS: Propolis is a resinous substance produced by the honeybee that has been adopted as a form of traditional medicine since ancient times.
31368546	6	5	theme	±	796:796	arg1	µg/ml					802:806	IC50 15 ± 3.2 µg/ml	788:806	IC50 15 ± 3.2 µg/ml	788:806	The PEEP inhibited the proliferation of RAW 264.7 cells with IC50 15 ± 3.2 µg/ml.
31368546	11	6	theme	plants	1406:1411	arg1	type					1398:1401	the type	1394:1401	the type of plants and climatic region	1394:1431	The main compounds found in propolis are typically various and depend on the type of plants and climatic region.
31368546	9	7	theme	PEEP	1016:1019	arg1	effects					1039:1045	the PEEP anti-inflammatory effects	1012:1045	the PEEP anti-inflammatory effects on RAW 264.7 cells	1012:1064	The obtained results supported the PEEP anti-inflammatory effects on RAW 264.7 cells may be applied via reducing ROS and NO production along with COX-2, IL-1β, and IL-6 expression.
31368546	4	8	theme	cell	567:570	arg1	viability					572:580	cell viability	567:580	cell viability	567:580	Cells were treated with different concentrations of PEEP and LPS, then cell viability, NO levels, and expression of inflammatory factors were evaluated.
31368546	4	8	theme	cell	567:570	arg1	concentrations					530:543	different concentrations	520:543	different concentrations of PEEP and LPS	520:559	Cells were treated with different concentrations of PEEP and LPS, then cell viability, NO levels, and expression of inflammatory factors were evaluated.
31368546	5	9	theme	phenolic	712:719	arg1	acid					721:724	phenolic acid	712:724	phenolic acid	712:724	HPLC analysis of PEEP indicated the presence of flavonoids and phenolic acid.
31368546	9	10	theme	IL-6	1145:1148	arg1	expression					1150:1159	COX-2, IL-1β, and IL-6 expression	1127:1159	COX-2, IL-1β, and IL-6 expression	1127:1159	The obtained results supported the PEEP anti-inflammatory effects on RAW 264.7 cells may be applied via reducing ROS and NO production along with COX-2, IL-1β, and IL-6 expression.
31368546	5	11	theme	HPLC	649:652	arg1	analysis					654:661	HPLC analysis	649:661	HPLC analysis of PEEP	649:669	HPLC analysis of PEEP indicated the presence of flavonoids and phenolic acid.
31368546	8	12	theme	IL-1β	941:945	arg1	expression					920:929	expression	920:929	expression of Cox-2, IL-1β and IL-6	920:954	Additionally, expression of Cox-2, IL-1β and IL-6 significantly decreased.
31368546	9	13	theme	anti-inflammatory	1021:1037	arg1	effects					1039:1045	the PEEP anti-inflammatory effects	1012:1045	the PEEP anti-inflammatory effects on RAW 264.7 cells	1012:1064	The obtained results supported the PEEP anti-inflammatory effects on RAW 264.7 cells may be applied via reducing ROS and NO production along with COX-2, IL-1β, and IL-6 expression.
31368546	4	14	theme	NO	583:584	arg1	concentrations					530:543	different concentrations	520:543	different concentrations of PEEP and LPS	520:559	Cells were treated with different concentrations of PEEP and LPS, then cell viability, NO levels, and expression of inflammatory factors were evaluated.
31368546	4	14	theme	NO	583:584	arg1	levels					586:591	NO levels	583:591	NO levels	583:591	Cells were treated with different concentrations of PEEP and LPS, then cell viability, NO levels, and expression of inflammatory factors were evaluated.
31368546	5	15	theme	acid	721:724	arg1	presence					685:692	the presence	681:692	the presence of flavonoids and phenolic acid	681:724	HPLC analysis of PEEP indicated the presence of flavonoids and phenolic acid.
31368546	6	16	theme	IC50	788:791	arg1	µg/ml					802:806	IC50 15 ± 3.2 µg/ml	788:806	IC50 15 ± 3.2 µg/ml	788:806	The PEEP inhibited the proliferation of RAW 264.7 cells with IC50 15 ± 3.2 µg/ml.
31368546	2	17	theme	immunomodulatory	298:313	arg1	effects					315:321	the immunomodulatory effects	294:321	the immunomodulatory effects of the Polur ethanol extract of propolis (PEEP) in murine macrophage (RAW 264.7) cells	294:408	In the current study, antioxidative and the immunomodulatory effects of the Polur ethanol extract of propolis (PEEP) in murine macrophage (RAW 264.7) cells were investigated.
31368546	8	18	theme	Cox-2	934:938	arg1	expression					920:929	expression	920:929	expression of Cox-2, IL-1β and IL-6	920:954	Additionally, expression of Cox-2, IL-1β and IL-6 significantly decreased.
31368546	3	19	theme	HPLC	481:484	arg1	analysis					486:493	HPLC analysis	481:493	HPLC analysis	481:493	Bioactive composition of the PEEP was determined by HPLC analysis.
31368546	0	20	theme	IL-1β	139:143	arg1	expressions					154:164	IL-1β and IL-6 expressions	139:164	IL-1β and IL-6 expressions in murine RAW 264.7 macrophages	139:196	Propolis attenuates lipopolysaccharide-induced inflammatory responses through intracellular ROS and NO levels along with downregulation of IL-1β and IL-6 expressions in murine RAW 264.7 macrophages.
31368546	12	21	dep	antioxidant	1535:1545	arg1	anticarcinogenic					1563:1578	anticarcinogenic	1563:1578	anticarcinogenic	1563:1578	In this respect, a wide spectrum of biological activities for propolis has been identified including antioxidant, antimicrobial, anticarcinogenic, anti-inflammatory, as well as antifungal properties.
31368546	12	21	dep	antioxidant	1535:1545	arg1	antimicrobial					1548:1560	antimicrobial	1548:1560	antimicrobial	1548:1560	In this respect, a wide spectrum of biological activities for propolis has been identified including antioxidant, antimicrobial, anticarcinogenic, anti-inflammatory, as well as antifungal properties.
31368546	12	21	dep	antioxidant	1535:1545	arg1	anti-inflammatory					1581:1597	anti-inflammatory	1581:1597	anti-inflammatory	1581:1597	In this respect, a wide spectrum of biological activities for propolis has been identified including antioxidant, antimicrobial, anticarcinogenic, anti-inflammatory, as well as antifungal properties.
31368546	9	22	theme	obtained	985:992	arg1	results					994:1000	The obtained results	981:1000	The obtained results	981:1000	The obtained results supported the PEEP anti-inflammatory effects on RAW 264.7 cells may be applied via reducing ROS and NO production along with COX-2, IL-1β, and IL-6 expression.
31368546	9	23	theme	COX-2	1127:1131	arg1	expression					1150:1159	COX-2, IL-1β, and IL-6 expression	1127:1159	COX-2, IL-1β, and IL-6 expression	1127:1159	The obtained results supported the PEEP anti-inflammatory effects on RAW 264.7 cells may be applied via reducing ROS and NO production along with COX-2, IL-1β, and IL-6 expression.
31368546	13	24	from	antioxidants	1689:1700	arg1	rich					1666:1669	rich	1666:1669	rich	1666:1669	This extraordinary substance is rich in flavonoids and antioxidants.
31368546	6	25	theme	cells	777:781	arg1	proliferation					750:762	the proliferation	746:762	the proliferation of RAW 264.7 cells	746:781	The PEEP inhibited the proliferation of RAW 264.7 cells with IC50 15 ± 3.2 µg/ml.
31368546	7	26	theme	oxygen	818:823	arg1	species					825:831	Reactive oxygen species	809:831	Reactive oxygen species (ROS)	809:837	Reactive oxygen species (ROS) and NO production was significantly reduced by 0.15 µg/ml of PEEP.
31368546	7	26	theme	oxygen	818:823	arg1	ROS					834:836	ROS	834:836	ROS	834:836	Reactive oxygen species (ROS) and NO production was significantly reduced by 0.15 µg/ml of PEEP.
31368546	7	27	theme	PEEP	900:903	arg1	µg/ml					891:895	0.15 µg/ml	886:895	0.15 µg/ml of PEEP	886:903	Reactive oxygen species (ROS) and NO production was significantly reduced by 0.15 µg/ml of PEEP.
31368546	2	28	theme	ethanol	336:342	arg1	extract					344:350	the Polur ethanol extract	326:350	the Polur ethanol extract of propolis (PEEP)	326:369	In the current study, antioxidative and the immunomodulatory effects of the Polur ethanol extract of propolis (PEEP) in murine macrophage (RAW 264.7) cells were investigated.
31368546	5	29	theme	PEEP	666:669	arg1	analysis					654:661	HPLC analysis	649:661	HPLC analysis of PEEP	649:669	HPLC analysis of PEEP indicated the presence of flavonoids and phenolic acid.
31368546	4	30	theme	LPS	557:559	arg1	concentrations					530:543	different concentrations	520:543	different concentrations of PEEP and LPS	520:559	Cells were treated with different concentrations of PEEP and LPS, then cell viability, NO levels, and expression of inflammatory factors were evaluated.
31368546	4	30	theme	LPS	557:559	arg1	levels					586:591	NO levels	583:591	NO levels	583:591	Cells were treated with different concentrations of PEEP and LPS, then cell viability, NO levels, and expression of inflammatory factors were evaluated.
31368546	4	30	theme	LPS	557:559	arg1	viability					572:580	cell viability	567:580	cell viability	567:580	Cells were treated with different concentrations of PEEP and LPS, then cell viability, NO levels, and expression of inflammatory factors were evaluated.
31368546	9	31	theme	RAW	1050:1052	arg1	cells					1060:1064	RAW 264.7 cells	1050:1064	RAW 264.7 cells	1050:1064	The obtained results supported the PEEP anti-inflammatory effects on RAW 264.7 cells may be applied via reducing ROS and NO production along with COX-2, IL-1β, and IL-6 expression.
31368546	6	32	theme	RAW	767:769	arg1	cells					777:781	RAW 264.7 cells	767:781	RAW 264.7 cells	767:781	The PEEP inhibited the proliferation of RAW 264.7 cells with IC50 15 ± 3.2 µg/ml.
31368546	2	33	theme	Polur	330:334	arg1	extract					344:350	the Polur ethanol extract	326:350	the Polur ethanol extract of propolis (PEEP)	326:369	In the current study, antioxidative and the immunomodulatory effects of the Polur ethanol extract of propolis (PEEP) in murine macrophage (RAW 264.7) cells were investigated.
31368546	2	34	theme	macrophage	381:390	arg1	cells					404:408	murine macrophage (RAW 264.7) cells	374:408	murine macrophage (RAW 264.7) cells	374:408	In the current study, antioxidative and the immunomodulatory effects of the Polur ethanol extract of propolis (PEEP) in murine macrophage (RAW 264.7) cells were investigated.
31368546	0	35	theme	inflammatory	47:58	arg1	responses					60:68	lipopolysaccharide-induced inflammatory responses	20:68	lipopolysaccharide-induced inflammatory responses	20:68	Propolis attenuates lipopolysaccharide-induced inflammatory responses through intracellular ROS and NO levels along with downregulation of IL-1β and IL-6 expressions in murine RAW 264.7 macrophages.
31368546	7	36	theme	0.15	886:889	arg1	µg/ml					891:895	0.15 µg/ml	886:895	0.15 µg/ml of PEEP	886:903	Reactive oxygen species (ROS) and NO production was significantly reduced by 0.15 µg/ml of PEEP.
31368546	0	37	theme	IL-6	149:152	arg1	expressions					154:164	IL-1β and IL-6 expressions	139:164	IL-1β and IL-6 expressions in murine RAW 264.7 macrophages	139:196	Propolis attenuates lipopolysaccharide-induced inflammatory responses through intracellular ROS and NO levels along with downregulation of IL-1β and IL-6 expressions in murine RAW 264.7 macrophages.
31368546	7	38	theme	Reactive	809:816	arg1	species					825:831	Reactive oxygen species	809:831	Reactive oxygen species (ROS)	809:837	Reactive oxygen species (ROS) and NO production was significantly reduced by 0.15 µg/ml of PEEP.
31368546	7	38	theme	Reactive	809:816	arg1	ROS					834:836	ROS	834:836	ROS	834:836	Reactive oxygen species (ROS) and NO production was significantly reduced by 0.15 µg/ml of PEEP.
31368546	1	39	theme	wide	214:217	arg1	spectrum					219:226	a wide spectrum	212:226	a wide spectrum of biological activities	212:251	Propolis had a wide spectrum of biological activities.
31368546	0	40	theme	lipopolysaccharide-induced	20:45	arg1	responses					60:68	lipopolysaccharide-induced inflammatory responses	20:68	lipopolysaccharide-induced inflammatory responses	20:68	Propolis attenuates lipopolysaccharide-induced inflammatory responses through intracellular ROS and NO levels along with downregulation of IL-1β and IL-6 expressions in murine RAW 264.7 macrophages.
31368546	9	41	theme	IL-1β	1134:1138	arg1	expression					1150:1159	COX-2, IL-1β, and IL-6 expression	1127:1159	COX-2, IL-1β, and IL-6 expression	1127:1159	The obtained results supported the PEEP anti-inflammatory effects on RAW 264.7 cells may be applied via reducing ROS and NO production along with COX-2, IL-1β, and IL-6 expression.
31368546	14	42	theme	human	1803:1807	arg1	health					1809:1814	human health	1803:1814	human health	1803:1814	Therefore, it is now widely used in foods and drinks with the claim that it can maintain or improve human health.
31368546	14	43	used	used	1731:1734	arg2	it					1714:1715	it	1714:1715	it	1714:1715	Therefore, it is now widely used in foods and drinks with the claim that it can maintain or improve human health.
31368546	5	44	theme	flavonoids	697:706	arg1	presence					685:692	the presence	681:692	the presence of flavonoids and phenolic acid	681:724	HPLC analysis of PEEP indicated the presence of flavonoids and phenolic acid.
31368546	1	45	contain	had	208:210	arg2	spectrum					219:226	a wide spectrum	212:226	a wide spectrum of biological activities	212:251	Propolis had a wide spectrum of biological activities.
31368546	1	45	contain	had	208:210	arg1	Propolis					199:206	Propolis	199:206	Propolis	199:206	Propolis had a wide spectrum of biological activities.
31368546	4	46	theme	factors	625:631	arg1	expression					598:607	expression	598:607	expression of inflammatory factors	598:631	Cells were treated with different concentrations of PEEP and LPS, then cell viability, NO levels, and expression of inflammatory factors were evaluated.
31368546	3	47	theme	Bioactive	429:437	arg1	composition					439:449	Bioactive composition	429:449	Bioactive composition of the PEEP	429:461	Bioactive composition of the PEEP was determined by HPLC analysis.
31368546	12	48	theme	antifungal	1611:1620	arg1	properties					1622:1631	antifungal properties	1611:1631	antifungal properties	1611:1631	In this respect, a wide spectrum of biological activities for propolis has been identified including antioxidant, antimicrobial, anticarcinogenic, anti-inflammatory, as well as antifungal properties.
31368546	0	49	theme	expressions	154:164	arg1	downregulation					121:134	downregulation	121:134	downregulation of IL-1β and IL-6 expressions in murine RAW 264.7 macrophages	121:196	Propolis attenuates lipopolysaccharide-induced inflammatory responses through intracellular ROS and NO levels along with downregulation of IL-1β and IL-6 expressions in murine RAW 264.7 macrophages.
31368546	1	50	theme	biological	231:240	arg1	activities					242:251	biological activities	231:251	biological activities	231:251	Propolis had a wide spectrum of biological activities.
31368546	4	51	theme	different	520:528	arg1	concentrations					530:543	different concentrations	520:543	different concentrations of PEEP and LPS	520:559	Cells were treated with different concentrations of PEEP and LPS, then cell viability, NO levels, and expression of inflammatory factors were evaluated.
31368546	4	51	theme	different	520:528	arg1	levels					586:591	NO levels	583:591	NO levels	583:591	Cells were treated with different concentrations of PEEP and LPS, then cell viability, NO levels, and expression of inflammatory factors were evaluated.
31368546	4	51	theme	different	520:528	arg1	viability					572:580	cell viability	567:580	cell viability	567:580	Cells were treated with different concentrations of PEEP and LPS, then cell viability, NO levels, and expression of inflammatory factors were evaluated.
31368546	0	52	theme	RAW	176:178	arg1	macrophages					186:196	murine RAW 264.7 macrophages	169:196	murine RAW 264.7 macrophages	169:196	Propolis attenuates lipopolysaccharide-induced inflammatory responses through intracellular ROS and NO levels along with downregulation of IL-1β and IL-6 expressions in murine RAW 264.7 macrophages.
31368546	11	53	located	found	1340:1344	arg2	various					1372:1378	various	1372:1378	various	1372:1378	The main compounds found in propolis are typically various and depend on the type of plants and climatic region.
31368546	11	53	located	found	1340:1344	arg2	compounds					1330:1338	The main compounds	1321:1338	The main compounds found in propolis	1321:1356	The main compounds found in propolis are typically various and depend on the type of plants and climatic region.
31368546	11	53	located	found	1340:1344	arg1	propolis					1349:1356	propolis	1349:1356	propolis	1349:1356	The main compounds found in propolis are typically various and depend on the type of plants and climatic region.
31368546	10	54	theme	ancient	1306:1312	arg1	times					1314:1318	ancient times	1306:1318	ancient times	1306:1318	PRACTICAL APPLICATIONS: Propolis is a resinous substance produced by the honeybee that has been adopted as a form of traditional medicine since ancient times.
31368546	11	55	theme	region	1426:1431	arg1	type					1398:1401	the type	1394:1401	the type of plants and climatic region	1394:1431	The main compounds found in propolis are typically various and depend on the type of plants and climatic region.
31368546	0	56	theme	intracellular	78:90	arg1	ROS					92:94	intracellular ROS	78:94	intracellular ROS	78:94	Propolis attenuates lipopolysaccharide-induced inflammatory responses through intracellular ROS and NO levels along with downregulation of IL-1β and IL-6 expressions in murine RAW 264.7 macrophages.
31368546	4	57	theme	PEEP	548:551	arg1	concentrations					530:543	different concentrations	520:543	different concentrations of PEEP and LPS	520:559	Cells were treated with different concentrations of PEEP and LPS, then cell viability, NO levels, and expression of inflammatory factors were evaluated.
31368546	4	57	theme	PEEP	548:551	arg1	levels					586:591	NO levels	583:591	NO levels	583:591	Cells were treated with different concentrations of PEEP and LPS, then cell viability, NO levels, and expression of inflammatory factors were evaluated.
31368546	4	57	theme	PEEP	548:551	arg1	viability					572:580	cell viability	567:580	cell viability	567:580	Cells were treated with different concentrations of PEEP and LPS, then cell viability, NO levels, and expression of inflammatory factors were evaluated.
31368546	0	58	theme	murine	169:174	arg1	macrophages					186:196	murine RAW 264.7 macrophages	169:196	murine RAW 264.7 macrophages	169:196	Propolis attenuates lipopolysaccharide-induced inflammatory responses through intracellular ROS and NO levels along with downregulation of IL-1β and IL-6 expressions in murine RAW 264.7 macrophages.
31368546	2	59	theme	propolis	355:362	arg1	extract					344:350	the Polur ethanol extract	326:350	the Polur ethanol extract of propolis (PEEP)	326:369	In the current study, antioxidative and the immunomodulatory effects of the Polur ethanol extract of propolis (PEEP) in murine macrophage (RAW 264.7) cells were investigated.
31368546	8	60	theme	IL-6	951:954	arg1	expression					920:929	expression	920:929	expression of Cox-2, IL-1β and IL-6	920:954	Additionally, expression of Cox-2, IL-1β and IL-6 significantly decreased.
31368546	12	61	theme	wide	1453:1456	arg1	spectrum					1458:1465	a wide spectrum	1451:1465	a wide spectrum of biological activities for propolis	1451:1503	In this respect, a wide spectrum of biological activities for propolis has been identified including antioxidant, antimicrobial, anticarcinogenic, anti-inflammatory, as well as antifungal properties.
31368546	11	62	theme	climatic	1417:1424	arg1	region					1426:1431	climatic region	1417:1431	climatic region	1417:1431	The main compounds found in propolis are typically various and depend on the type of plants and climatic region.
31368546	1	63	theme	activities	242:251	arg1	spectrum					219:226	a wide spectrum	212:226	a wide spectrum of biological activities	212:251	Propolis had a wide spectrum of biological activities.
31368546	13	64	theme	extraordinary	1639:1651	arg1	substance					1653:1661	This extraordinary substance	1634:1661	This extraordinary substance	1634:1661	This extraordinary substance is rich in flavonoids and antioxidants.
31368546	0	65	from	downregulation	121:134	arg1	macrophages					186:196	murine RAW 264.7 macrophages	169:196	murine RAW 264.7 macrophages	169:196	Propolis attenuates lipopolysaccharide-induced inflammatory responses through intracellular ROS and NO levels along with downregulation of IL-1β and IL-6 expressions in murine RAW 264.7 macrophages.
31368546	9	66	theme	NO	1102:1103	arg1	production					1105:1114	NO production	1102:1114	NO production	1102:1114	The obtained results supported the PEEP anti-inflammatory effects on RAW 264.7 cells may be applied via reducing ROS and NO production along with COX-2, IL-1β, and IL-6 expression.
31368546	2	67	theme	extract	344:350	arg1	effects					315:321	the immunomodulatory effects	294:321	the immunomodulatory effects of the Polur ethanol extract of propolis (PEEP) in murine macrophage (RAW 264.7) cells	294:408	In the current study, antioxidative and the immunomodulatory effects of the Polur ethanol extract of propolis (PEEP) in murine macrophage (RAW 264.7) cells were investigated.
31368546	2	68	from	effects	315:321	arg1	cells					404:408	murine macrophage (RAW 264.7) cells	374:408	murine macrophage (RAW 264.7) cells	374:408	In the current study, antioxidative and the immunomodulatory effects of the Polur ethanol extract of propolis (PEEP) in murine macrophage (RAW 264.7) cells were investigated.
31368546	0	69	from	expressions	154:164	arg1	macrophages					186:196	murine RAW 264.7 macrophages	169:196	murine RAW 264.7 macrophages	169:196	Propolis attenuates lipopolysaccharide-induced inflammatory responses through intracellular ROS and NO levels along with downregulation of IL-1β and IL-6 expressions in murine RAW 264.7 macrophages.
31368546	3	70	theme	PEEP	458:461	arg1	composition					439:449	Bioactive composition	429:449	Bioactive composition of the PEEP	429:461	Bioactive composition of the PEEP was determined by HPLC analysis.
31368546	9	71	from	effects	1039:1045	arg1	cells					1060:1064	RAW 264.7 cells	1050:1064	RAW 264.7 cells	1050:1064	The obtained results supported the PEEP anti-inflammatory effects on RAW 264.7 cells may be applied via reducing ROS and NO production along with COX-2, IL-1β, and IL-6 expression.
31368546	4	72	theme	inflammatory	612:623	arg1	factors					625:631	inflammatory factors	612:631	inflammatory factors	612:631	Cells were treated with different concentrations of PEEP and LPS, then cell viability, NO levels, and expression of inflammatory factors were evaluated.
31368546	2	73	theme	murine	374:379	arg1	RAW					393:395	RAW 264.7	393:401	RAW 264.7	393:401	In the current study, antioxidative and the immunomodulatory effects of the Polur ethanol extract of propolis (PEEP) in murine macrophage (RAW 264.7) cells were investigated.
31368546	2	73	theme	murine	374:379	arg1	macrophage					381:390	murine macrophage	374:390	murine macrophage (RAW 264.7) cells	374:408	In the current study, antioxidative and the immunomodulatory effects of the Polur ethanol extract of propolis (PEEP) in murine macrophage (RAW 264.7) cells were investigated.
31368546	10	74	theme	PRACTICAL	1162:1170	arg1	APPLICATIONS					1172:1183	PRACTICAL APPLICATIONS	1162:1183	PRACTICAL APPLICATIONS: Propolis is a resinous substance produced by the honeybee that has been adopted as a form of traditional medicine since ancient times.	1162:1319	PRACTICAL APPLICATIONS: Propolis is a resinous substance produced by the honeybee that has been adopted as a form of traditional medicine since ancient times.
31368546	12	75	theme	activities	1481:1490	arg1	spectrum					1458:1465	a wide spectrum	1451:1465	a wide spectrum of biological activities for propolis	1451:1503	In this respect, a wide spectrum of biological activities for propolis has been identified including antioxidant, antimicrobial, anticarcinogenic, anti-inflammatory, as well as antifungal properties.
31368546	11	76	theme	main	1325:1328	arg1	various					1372:1378	various	1372:1378	various	1372:1378	The main compounds found in propolis are typically various and depend on the type of plants and climatic region.
31368546	11	76	theme	main	1325:1328	arg1	compounds					1330:1338	The main compounds	1321:1338	The main compounds found in propolis	1321:1356	The main compounds found in propolis are typically various and depend on the type of plants and climatic region.
31368546	13	77	from	flavonoids	1674:1683	arg1	rich					1666:1669	rich	1666:1669	rich	1666:1669	This extraordinary substance is rich in flavonoids and antioxidants.
31368546	2	78	theme	current	261:267	arg1	study					269:273	the current study	257:273	the current study	257:273	In the current study, antioxidative and the immunomodulatory effects of the Polur ethanol extract of propolis (PEEP) in murine macrophage (RAW 264.7) cells were investigated.
31368546	10	79	theme	medicine	1291:1298	arg1	form					1271:1274	a form	1269:1274	a form of traditional medicine since ancient times	1269:1318	PRACTICAL APPLICATIONS: Propolis is a resinous substance produced by the honeybee that has been adopted as a form of traditional medicine since ancient times.
31368546	10	79	theme	medicine	1291:1298	arg1	honeybee					1235:1242	the honeybee	1231:1242	the honeybee that has been adopted as a form of traditional medicine since ancient times	1231:1318	PRACTICAL APPLICATIONS: Propolis is a resinous substance produced by the honeybee that has been adopted as a form of traditional medicine since ancient times.
31368546	7	80	theme	NO	843:844	arg1	production					846:855	NO production	843:855	NO production	843:855	Reactive oxygen species (ROS) and NO production was significantly reduced by 0.15 µg/ml of PEEP.
31368546	12	81	theme	biological	1470:1479	arg1	activities					1481:1490	biological activities	1470:1490	biological activities	1470:1490	In this respect, a wide spectrum of biological activities for propolis has been identified including antioxidant, antimicrobial, anticarcinogenic, anti-inflammatory, as well as antifungal properties.
29886193	4	0	theme	whole-body	970:979	arg1	sensitivity					989:999	whole-body insulin sensitivity	970:999	whole-body insulin sensitivity	970:999	Oral administration of kaempferol (50 mg/kg/day), which is the human equivalent dose of 240 mg/day for an average 60 kg human, significantly improved blood glucose control in obese mice, which was associated with reduced hepatic glucose production and improved whole-body insulin sensitivity without altering body weight gain, food consumption or adiposity.
29886193	5	1	theme	hexokinase	1119:1128	arg1	activity					1130:1137	Akt and hexokinase activity	1111:1137	activity	1130:1137	In addition, kaempferol treatment increased Akt and hexokinase activity, but decreased pyruvate carboxylase (PC) and glucose-6 phosphatase activity in the liver without altering their protein expression.
29886193	6	2	theme	PC	1306:1307	arg1	activity					1309:1316	PC activity	1306:1316	PC activity	1306:1316	Consistently, kaempferol decreased PC activity and suppressed gluconeogenesis in HepG2 cells as well as primary hepatocytes isolated from the livers of obese mice.
29886193	4	3	theme	Oral	709:712	arg1	mg/kg/day					747:755	50 mg/kg/day	744:755	50 mg/kg/day	744:755	Oral administration of kaempferol (50 mg/kg/day), which is the human equivalent dose of 240 mg/day for an average 60 kg human, significantly improved blood glucose control in obese mice, which was associated with reduced hepatic glucose production and improved whole-body insulin sensitivity without altering body weight gain, food consumption or adiposity.
29886193	4	3	theme	Oral	709:712	arg1	administration					714:727	Oral administration	709:727	Oral administration	709:727	Oral administration of kaempferol (50 mg/kg/day), which is the human equivalent dose of 240 mg/day for an average 60 kg human, significantly improved blood glucose control in obese mice, which was associated with reduced hepatic glucose production and improved whole-body insulin sensitivity without altering body weight gain, food consumption or adiposity.
29886193	4	3	theme	Oral	709:712	arg1	dose					789:792	the human equivalent dose	768:792	the human equivalent dose of 240 mg/day for an average 60 kg human	768:833	Oral administration of kaempferol (50 mg/kg/day), which is the human equivalent dose of 240 mg/day for an average 60 kg human, significantly improved blood glucose control in obese mice, which was associated with reduced hepatic glucose production and improved whole-body insulin sensitivity without altering body weight gain, food consumption or adiposity.
29886193	4	4	theme	food	1036:1039	arg1	consumption					1041:1051	food consumption	1036:1051	food consumption	1036:1051	Oral administration of kaempferol (50 mg/kg/day), which is the human equivalent dose of 240 mg/day for an average 60 kg human, significantly improved blood glucose control in obese mice, which was associated with reduced hepatic glucose production and improved whole-body insulin sensitivity without altering body weight gain, food consumption or adiposity.
29886193	5	5	theme	glucose-6	1184:1192	arg1	activity					1206:1213	glucose-6 phosphatase activity	1184:1213	glucose-6 phosphatase activity in the liver	1184:1226	In addition, kaempferol treatment increased Akt and hexokinase activity, but decreased pyruvate carboxylase (PC) and glucose-6 phosphatase activity in the liver without altering their protein expression.
29886193	0	6	theme	insulin	103:109	arg1	sensitivity					111:121	hepatic insulin sensitivity	95:121	hepatic insulin sensitivity in diet-induced obese mice	95:148	Kaempferol ameliorates hyperglycemia through suppressing hepatic gluconeogenesis and enhancing hepatic insulin sensitivity in diet-induced obese mice.
29886193	3	7	theme	such	695:698	arg1	effects					700:706	such effects	695:706	such effects	695:706	In this study, we explored whether kaempferol, a flavonoid isolated from Gink go biloba, is able to regulate hepatic gluconeogenesis and blood glucose homeostasis in high-fat diet-fed obese mice and further explored the underlying mechanism by which it elicits such effects.
29886193	5	8	from	carboxylase	1163:1173	arg1	liver					1222:1226	the liver	1218:1226	the liver	1218:1226	In addition, kaempferol treatment increased Akt and hexokinase activity, but decreased pyruvate carboxylase (PC) and glucose-6 phosphatase activity in the liver without altering their protein expression.
29886193	2	9	from	increase	336:343	arg1	production					364:373	hepatic glucose production	348:373	hepatic glucose production	348:373	In particular, hepatic IR contributes to the increase in hepatic glucose production and consequently the development of fasting hyperglycemia.
29886193	2	9	from	increase	336:343	arg1	development					396:406	consequently the development	379:406	consequently the development of fasting hyperglycemia	379:431	In particular, hepatic IR contributes to the increase in hepatic glucose production and consequently the development of fasting hyperglycemia.
29886193	2	10	theme	glucose	356:362	arg1	production					364:373	hepatic glucose production	348:373	hepatic glucose production	348:373	In particular, hepatic IR contributes to the increase in hepatic glucose production and consequently the development of fasting hyperglycemia.
29886193	1	11	theme	other	264:268	arg1	disorders					280:288	other metabolic disorders	264:288	other metabolic disorders	264:288	Obesity-associated insulin resistance (IR) is a major risk factor for developing type 2 diabetes and an array of other metabolic disorders.
29886193	0	12	theme	diet-induced	126:137	arg1	mice					145:148	diet-induced obese mice	126:148	diet-induced obese mice	126:148	Kaempferol ameliorates hyperglycemia through suppressing hepatic gluconeogenesis and enhancing hepatic insulin sensitivity in diet-induced obese mice.
29886193	7	13	theme	direct	1478:1483	arg1	kaempferol					1462:1471	kaempferol	1462:1471	kaempferol	1462:1471	Furthermore, we found that kaempferol is a direct inhibitor of PC.
29886193	7	13	theme	direct	1478:1483	arg1	inhibitor					1485:1493	a direct inhibitor	1476:1493	a direct inhibitor of PC	1476:1499	Furthermore, we found that kaempferol is a direct inhibitor of PC.
29886193	3	14	theme	underlying	654:663	arg1	mechanism					665:673	the underlying mechanism	650:673	the underlying mechanism by which it elicits such effects	650:706	In this study, we explored whether kaempferol, a flavonoid isolated from Gink go biloba, is able to regulate hepatic gluconeogenesis and blood glucose homeostasis in high-fat diet-fed obese mice and further explored the underlying mechanism by which it elicits such effects.
29886193	8	15	theme	antidiabetic	1570:1581	arg1	compound					1583:1590	a naturally occurring antidiabetic compound	1548:1590	a naturally occurring antidiabetic compound that acts by suppressing glucose production and improving insulin sensitivity	1548:1668	These findings suggest that kaempferol may be a naturally occurring antidiabetic compound that acts by suppressing glucose production and improving insulin sensitivity.
29886193	8	15	theme	antidiabetic	1570:1581	arg1	kaempferol					1530:1539	kaempferol	1530:1539	kaempferol	1530:1539	These findings suggest that kaempferol may be a naturally occurring antidiabetic compound that acts by suppressing glucose production and improving insulin sensitivity.
29886193	1	16	theme	metabolic	270:278	arg1	disorders					280:288	other metabolic disorders	264:288	other metabolic disorders	264:288	Obesity-associated insulin resistance (IR) is a major risk factor for developing type 2 diabetes and an array of other metabolic disorders.
29886193	5	17	theme	kaempferol	1080:1089	arg1	treatment					1091:1099	kaempferol treatment	1080:1099	kaempferol treatment	1080:1099	In addition, kaempferol treatment increased Akt and hexokinase activity, but decreased pyruvate carboxylase (PC) and glucose-6 phosphatase activity in the liver without altering their protein expression.
29886193	4	18	theme	blood	859:863	arg1	control					873:879	blood glucose control	859:879	blood glucose control	859:879	Oral administration of kaempferol (50 mg/kg/day), which is the human equivalent dose of 240 mg/day for an average 60 kg human, significantly improved blood glucose control in obese mice, which was associated with reduced hepatic glucose production and improved whole-body insulin sensitivity without altering body weight gain, food consumption or adiposity.
29886193	8	19	theme	insulin	1650:1656	arg1	sensitivity					1658:1668	insulin sensitivity	1650:1668	insulin sensitivity	1650:1668	These findings suggest that kaempferol may be a naturally occurring antidiabetic compound that acts by suppressing glucose production and improving insulin sensitivity.
29886193	3	20	theme	hepatic	543:549	arg1	gluconeogenesis					551:565	hepatic gluconeogenesis	543:565	hepatic gluconeogenesis	543:565	In this study, we explored whether kaempferol, a flavonoid isolated from Gink go biloba, is able to regulate hepatic gluconeogenesis and blood glucose homeostasis in high-fat diet-fed obese mice and further explored the underlying mechanism by which it elicits such effects.
29886193	1	21	theme	disorders	280:288	arg1	diabetes					239:246	type 2 diabetes	232:246	type 2 diabetes	232:246	Obesity-associated insulin resistance (IR) is a major risk factor for developing type 2 diabetes and an array of other metabolic disorders.
29886193	1	21	theme	disorders	280:288	arg1	array					255:259	an array	252:259	an array of other metabolic disorders	252:288	Obesity-associated insulin resistance (IR) is a major risk factor for developing type 2 diabetes and an array of other metabolic disorders.
29886193	4	22	theme	glucose	938:944	arg1	production					946:955	reduced hepatic glucose production	922:955	reduced hepatic glucose production	922:955	Oral administration of kaempferol (50 mg/kg/day), which is the human equivalent dose of 240 mg/day for an average 60 kg human, significantly improved blood glucose control in obese mice, which was associated with reduced hepatic glucose production and improved whole-body insulin sensitivity without altering body weight gain, food consumption or adiposity.
29886193	9	23	theme	hepatic	1697:1703	arg1	gluconeogenesis					1705:1719	hepatic gluconeogenesis	1697:1719	hepatic gluconeogenesis	1697:1719	Kaempferol suppression of hepatic gluconeogenesis is due to its direct inhibitory action on the enzymatic activity of PC.
29886193	5	24	theme	protein	1251:1257	arg1	expression					1259:1268	their protein expression	1245:1268	their protein expression	1245:1268	In addition, kaempferol treatment increased Akt and hexokinase activity, but decreased pyruvate carboxylase (PC) and glucose-6 phosphatase activity in the liver without altering their protein expression.
29886193	4	25	theme	human	772:776	arg1	dose					789:792	the human equivalent dose	768:792	the human equivalent dose of 240 mg/day for an average 60 kg human	768:833	Oral administration of kaempferol (50 mg/kg/day), which is the human equivalent dose of 240 mg/day for an average 60 kg human, significantly improved blood glucose control in obese mice, which was associated with reduced hepatic glucose production and improved whole-body insulin sensitivity without altering body weight gain, food consumption or adiposity.
29886193	4	25	theme	human	772:776	arg1	administration					714:727	Oral administration	709:727	Oral administration	709:727	Oral administration of kaempferol (50 mg/kg/day), which is the human equivalent dose of 240 mg/day for an average 60 kg human, significantly improved blood glucose control in obese mice, which was associated with reduced hepatic glucose production and improved whole-body insulin sensitivity without altering body weight gain, food consumption or adiposity.
29886193	2	26	theme	fasting	411:417	arg1	hyperglycemia					419:431	fasting hyperglycemia	411:431	fasting hyperglycemia	411:431	In particular, hepatic IR contributes to the increase in hepatic glucose production and consequently the development of fasting hyperglycemia.
29886193	0	27	theme	obese	139:143	arg1	mice					145:148	diet-induced obese mice	126:148	diet-induced obese mice	126:148	Kaempferol ameliorates hyperglycemia through suppressing hepatic gluconeogenesis and enhancing hepatic insulin sensitivity in diet-induced obese mice.
29886193	6	28	theme	mice	1429:1432	arg1	livers					1413:1418	the livers	1409:1418	the livers of obese mice	1409:1432	Consistently, kaempferol decreased PC activity and suppressed gluconeogenesis in HepG2 cells as well as primary hepatocytes isolated from the livers of obese mice.
29886193	3	29	dep	Gink	507:510	arg1	go					512:513	go	512:513	go biloba	512:520	In this study, we explored whether kaempferol, a flavonoid isolated from Gink go biloba, is able to regulate hepatic gluconeogenesis and blood glucose homeostasis in high-fat diet-fed obese mice and further explored the underlying mechanism by which it elicits such effects.
29886193	1	30	theme	major	199:203	arg1	factor					210:215	a major risk factor	197:215	a major risk factor for developing type 2 diabetes and an array of other metabolic disorders	197:288	Obesity-associated insulin resistance (IR) is a major risk factor for developing type 2 diabetes and an array of other metabolic disorders.
29886193	1	30	theme	major	199:203	arg1	resistance					178:187	Obesity-associated insulin resistance	151:187	Obesity-associated insulin resistance (IR)	151:192	Obesity-associated insulin resistance (IR) is a major risk factor for developing type 2 diabetes and an array of other metabolic disorders.
29886193	4	31	theme	weight	1023:1028	arg1	gain					1030:1033	body weight gain	1018:1033	body weight gain	1018:1033	Oral administration of kaempferol (50 mg/kg/day), which is the human equivalent dose of 240 mg/day for an average 60 kg human, significantly improved blood glucose control in obese mice, which was associated with reduced hepatic glucose production and improved whole-body insulin sensitivity without altering body weight gain, food consumption or adiposity.
29886193	5	32	theme	Akt	1111:1113	arg1	activity					1130:1137	Akt and hexokinase activity	1111:1137	activity	1130:1137	In addition, kaempferol treatment increased Akt and hexokinase activity, but decreased pyruvate carboxylase (PC) and glucose-6 phosphatase activity in the liver without altering their protein expression.
29886193	3	33	theme	blood	571:575	arg1	homeostasis					585:595	blood glucose homeostasis	571:595	blood glucose homeostasis	571:595	In this study, we explored whether kaempferol, a flavonoid isolated from Gink go biloba, is able to regulate hepatic gluconeogenesis and blood glucose homeostasis in high-fat diet-fed obese mice and further explored the underlying mechanism by which it elicits such effects.
29886193	1	34	theme	risk	205:208	arg1	factor					210:215	a major risk factor	197:215	a major risk factor for developing type 2 diabetes and an array of other metabolic disorders	197:288	Obesity-associated insulin resistance (IR) is a major risk factor for developing type 2 diabetes and an array of other metabolic disorders.
29886193	1	34	theme	risk	205:208	arg1	resistance					178:187	Obesity-associated insulin resistance	151:187	Obesity-associated insulin resistance (IR)	151:192	Obesity-associated insulin resistance (IR) is a major risk factor for developing type 2 diabetes and an array of other metabolic disorders.
29886193	4	35	theme	body	1018:1021	arg1	gain					1030:1033	body weight gain	1018:1033	body weight gain	1018:1033	Oral administration of kaempferol (50 mg/kg/day), which is the human equivalent dose of 240 mg/day for an average 60 kg human, significantly improved blood glucose control in obese mice, which was associated with reduced hepatic glucose production and improved whole-body insulin sensitivity without altering body weight gain, food consumption or adiposity.
29886193	4	36	theme	human	829:833	arg1	average					815:821	an average	812:821	an average 60 kg human	812:833	Oral administration of kaempferol (50 mg/kg/day), which is the human equivalent dose of 240 mg/day for an average 60 kg human, significantly improved blood glucose control in obese mice, which was associated with reduced hepatic glucose production and improved whole-body insulin sensitivity without altering body weight gain, food consumption or adiposity.
29886193	3	37	theme	glucose	577:583	arg1	homeostasis					585:595	blood glucose homeostasis	571:595	blood glucose homeostasis	571:595	In this study, we explored whether kaempferol, a flavonoid isolated from Gink go biloba, is able to regulate hepatic gluconeogenesis and blood glucose homeostasis in high-fat diet-fed obese mice and further explored the underlying mechanism by which it elicits such effects.
29886193	3	38	from	homeostasis	585:595	arg1	mice					624:627	high-fat diet-fed obese mice	600:627	high-fat diet-fed obese mice	600:627	In this study, we explored whether kaempferol, a flavonoid isolated from Gink go biloba, is able to regulate hepatic gluconeogenesis and blood glucose homeostasis in high-fat diet-fed obese mice and further explored the underlying mechanism by which it elicits such effects.
29886193	9	39	theme	Kaempferol	1671:1680	arg1	suppression					1682:1692	Kaempferol suppression	1671:1692	Kaempferol suppression of hepatic gluconeogenesis	1671:1719	Kaempferol suppression of hepatic gluconeogenesis is due to its direct inhibitory action on the enzymatic activity of PC.
29886193	6	40	from	gluconeogenesis	1333:1347	arg1	hepatocytes					1383:1393	primary hepatocytes	1375:1393	HepG2 cells as well as primary hepatocytes isolated from the livers of obese mice	1352:1432	Consistently, kaempferol decreased PC activity and suppressed gluconeogenesis in HepG2 cells as well as primary hepatocytes isolated from the livers of obese mice.
29886193	6	40	from	gluconeogenesis	1333:1347	arg1	cells					1358:1362	HepG2 cells	1352:1362	HepG2 cells as well as primary hepatocytes isolated from the livers of obese mice	1352:1432	Consistently, kaempferol decreased PC activity and suppressed gluconeogenesis in HepG2 cells as well as primary hepatocytes isolated from the livers of obese mice.
29886193	5	41	from	activity	1206:1213	arg1	liver					1222:1226	the liver	1218:1226	the liver	1218:1226	In addition, kaempferol treatment increased Akt and hexokinase activity, but decreased pyruvate carboxylase (PC) and glucose-6 phosphatase activity in the liver without altering their protein expression.
29886193	6	42	attach	isolated	1395:1402	arg1	livers					1413:1418	the livers	1409:1418	the livers of obese mice	1409:1432	Consistently, kaempferol decreased PC activity and suppressed gluconeogenesis in HepG2 cells as well as primary hepatocytes isolated from the livers of obese mice.
29886193	6	42	attach	isolated	1395:1402	arg2	hepatocytes					1383:1393	primary hepatocytes	1375:1393	HepG2 cells as well as primary hepatocytes isolated from the livers of obese mice	1352:1432	Consistently, kaempferol decreased PC activity and suppressed gluconeogenesis in HepG2 cells as well as primary hepatocytes isolated from the livers of obese mice.
29886193	8	43	theme	occurring	1560:1568	arg1	compound					1583:1590	a naturally occurring antidiabetic compound	1548:1590	a naturally occurring antidiabetic compound that acts by suppressing glucose production and improving insulin sensitivity	1548:1668	These findings suggest that kaempferol may be a naturally occurring antidiabetic compound that acts by suppressing glucose production and improving insulin sensitivity.
29886193	8	43	theme	occurring	1560:1568	arg1	kaempferol					1530:1539	kaempferol	1530:1539	kaempferol	1530:1539	These findings suggest that kaempferol may be a naturally occurring antidiabetic compound that acts by suppressing glucose production and improving insulin sensitivity.
29886193	6	44	theme	HepG2	1352:1356	arg1	cells					1358:1362	HepG2 cells	1352:1362	HepG2 cells as well as primary hepatocytes isolated from the livers of obese mice	1352:1432	Consistently, kaempferol decreased PC activity and suppressed gluconeogenesis in HepG2 cells as well as primary hepatocytes isolated from the livers of obese mice.
29886193	0	45	from	sensitivity	111:121	arg1	mice					145:148	diet-induced obese mice	126:148	diet-induced obese mice	126:148	Kaempferol ameliorates hyperglycemia through suppressing hepatic gluconeogenesis and enhancing hepatic insulin sensitivity in diet-induced obese mice.
29886193	2	46	theme	hepatic	306:312	arg1	IR					314:315	hepatic IR	306:315	hepatic IR	306:315	In particular, hepatic IR contributes to the increase in hepatic glucose production and consequently the development of fasting hyperglycemia.
29886193	0	47	theme	hepatic	57:63	arg1	gluconeogenesis					65:79	hepatic gluconeogenesis	57:79	hepatic gluconeogenesis	57:79	Kaempferol ameliorates hyperglycemia through suppressing hepatic gluconeogenesis and enhancing hepatic insulin sensitivity in diet-induced obese mice.
29886193	9	48	theme	direct	1735:1740	arg1	action					1753:1758	its direct inhibitory action	1731:1758	its direct inhibitory action on the enzymatic activity of PC	1731:1790	Kaempferol suppression of hepatic gluconeogenesis is due to its direct inhibitory action on the enzymatic activity of PC.
29886193	3	49	theme	high-fat	600:607	arg1	mice					624:627	high-fat diet-fed obese mice	600:627	high-fat diet-fed obese mice	600:627	In this study, we explored whether kaempferol, a flavonoid isolated from Gink go biloba, is able to regulate hepatic gluconeogenesis and blood glucose homeostasis in high-fat diet-fed obese mice and further explored the underlying mechanism by which it elicits such effects.
29886193	1	50	theme	type	232:235	arg1	diabetes					239:246	type 2 diabetes	232:246	type 2 diabetes	232:246	Obesity-associated insulin resistance (IR) is a major risk factor for developing type 2 diabetes and an array of other metabolic disorders.
29886193	4	51	theme	insulin	981:987	arg1	sensitivity					989:999	whole-body insulin sensitivity	970:999	whole-body insulin sensitivity	970:999	Oral administration of kaempferol (50 mg/kg/day), which is the human equivalent dose of 240 mg/day for an average 60 kg human, significantly improved blood glucose control in obese mice, which was associated with reduced hepatic glucose production and improved whole-body insulin sensitivity without altering body weight gain, food consumption or adiposity.
29886193	5	52	theme	phosphatase	1194:1204	arg1	activity					1206:1213	glucose-6 phosphatase activity	1184:1213	glucose-6 phosphatase activity in the liver	1184:1226	In addition, kaempferol treatment increased Akt and hexokinase activity, but decreased pyruvate carboxylase (PC) and glucose-6 phosphatase activity in the liver without altering their protein expression.
29886193	3	53	theme	diet-fed	609:616	arg1	mice					624:627	high-fat diet-fed obese mice	600:627	high-fat diet-fed obese mice	600:627	In this study, we explored whether kaempferol, a flavonoid isolated from Gink go biloba, is able to regulate hepatic gluconeogenesis and blood glucose homeostasis in high-fat diet-fed obese mice and further explored the underlying mechanism by which it elicits such effects.
29886193	2	54	theme	hyperglycemia	419:431	arg1	production					364:373	hepatic glucose production	348:373	hepatic glucose production	348:373	In particular, hepatic IR contributes to the increase in hepatic glucose production and consequently the development of fasting hyperglycemia.
29886193	2	54	theme	hyperglycemia	419:431	arg1	development					396:406	consequently the development	379:406	consequently the development of fasting hyperglycemia	379:431	In particular, hepatic IR contributes to the increase in hepatic glucose production and consequently the development of fasting hyperglycemia.
29886193	9	55	theme	enzymatic	1767:1775	arg1	activity					1777:1784	the enzymatic activity	1763:1784	the enzymatic activity of PC	1763:1790	Kaempferol suppression of hepatic gluconeogenesis is due to its direct inhibitory action on the enzymatic activity of PC.
29886193	4	56	theme	reduced	922:928	arg1	production					946:955	reduced hepatic glucose production	922:955	reduced hepatic glucose production	922:955	Oral administration of kaempferol (50 mg/kg/day), which is the human equivalent dose of 240 mg/day for an average 60 kg human, significantly improved blood glucose control in obese mice, which was associated with reduced hepatic glucose production and improved whole-body insulin sensitivity without altering body weight gain, food consumption or adiposity.
29886193	4	57	theme	glucose	865:871	arg1	control					873:879	blood glucose control	859:879	blood glucose control	859:879	Oral administration of kaempferol (50 mg/kg/day), which is the human equivalent dose of 240 mg/day for an average 60 kg human, significantly improved blood glucose control in obese mice, which was associated with reduced hepatic glucose production and improved whole-body insulin sensitivity without altering body weight gain, food consumption or adiposity.
29886193	6	58	theme	primary	1375:1381	arg1	hepatocytes					1383:1393	primary hepatocytes	1375:1393	HepG2 cells as well as primary hepatocytes isolated from the livers of obese mice	1352:1432	Consistently, kaempferol decreased PC activity and suppressed gluconeogenesis in HepG2 cells as well as primary hepatocytes isolated from the livers of obese mice.
29886193	2	59	theme	hepatic	348:354	arg1	production					364:373	hepatic glucose production	348:373	hepatic glucose production	348:373	In particular, hepatic IR contributes to the increase in hepatic glucose production and consequently the development of fasting hyperglycemia.
29886193	9	60	theme	inhibitory	1742:1751	arg1	action					1753:1758	its direct inhibitory action	1731:1758	its direct inhibitory action on the enzymatic activity of PC	1731:1790	Kaempferol suppression of hepatic gluconeogenesis is due to its direct inhibitory action on the enzymatic activity of PC.
29886193	5	61	theme	pyruvate	1154:1161	arg1	PC					1176:1177	PC	1176:1177	PC	1176:1177	In addition, kaempferol treatment increased Akt and hexokinase activity, but decreased pyruvate carboxylase (PC) and glucose-6 phosphatase activity in the liver without altering their protein expression.
29886193	5	61	theme	pyruvate	1154:1161	arg1	carboxylase					1163:1173	pyruvate carboxylase	1154:1173	pyruvate carboxylase (PC)	1154:1178	In addition, kaempferol treatment increased Akt and hexokinase activity, but decreased pyruvate carboxylase (PC) and glucose-6 phosphatase activity in the liver without altering their protein expression.
29886193	9	62	theme	gluconeogenesis	1705:1719	arg1	suppression					1682:1692	Kaempferol suppression	1671:1692	Kaempferol suppression of hepatic gluconeogenesis	1671:1719	Kaempferol suppression of hepatic gluconeogenesis is due to its direct inhibitory action on the enzymatic activity of PC.
29886193	1	63	theme	Obesity-associated	151:168	arg1	IR					190:191	IR	190:191	IR	190:191	Obesity-associated insulin resistance (IR) is a major risk factor for developing type 2 diabetes and an array of other metabolic disorders.
29886193	1	63	theme	Obesity-associated	151:168	arg1	resistance					178:187	Obesity-associated insulin resistance	151:187	Obesity-associated insulin resistance (IR)	151:192	Obesity-associated insulin resistance (IR) is a major risk factor for developing type 2 diabetes and an array of other metabolic disorders.
29886193	1	63	theme	Obesity-associated	151:168	arg1	factor					210:215	a major risk factor	197:215	a major risk factor for developing type 2 diabetes and an array of other metabolic disorders	197:288	Obesity-associated insulin resistance (IR) is a major risk factor for developing type 2 diabetes and an array of other metabolic disorders.
29886193	3	64	from	gluconeogenesis	551:565	arg1	mice					624:627	high-fat diet-fed obese mice	600:627	high-fat diet-fed obese mice	600:627	In this study, we explored whether kaempferol, a flavonoid isolated from Gink go biloba, is able to regulate hepatic gluconeogenesis and blood glucose homeostasis in high-fat diet-fed obese mice and further explored the underlying mechanism by which it elicits such effects.
29886193	6	65	theme	obese	1423:1427	arg1	mice					1429:1432	obese mice	1423:1432	obese mice	1423:1432	Consistently, kaempferol decreased PC activity and suppressed gluconeogenesis in HepG2 cells as well as primary hepatocytes isolated from the livers of obese mice.
29886193	4	66	theme	mg/day	801:806	arg1	dose					789:792	the human equivalent dose	768:792	the human equivalent dose of 240 mg/day for an average 60 kg human	768:833	Oral administration of kaempferol (50 mg/kg/day), which is the human equivalent dose of 240 mg/day for an average 60 kg human, significantly improved blood glucose control in obese mice, which was associated with reduced hepatic glucose production and improved whole-body insulin sensitivity without altering body weight gain, food consumption or adiposity.
29886193	4	66	theme	mg/day	801:806	arg1	administration					714:727	Oral administration	709:727	Oral administration	709:727	Oral administration of kaempferol (50 mg/kg/day), which is the human equivalent dose of 240 mg/day for an average 60 kg human, significantly improved blood glucose control in obese mice, which was associated with reduced hepatic glucose production and improved whole-body insulin sensitivity without altering body weight gain, food consumption or adiposity.
29886193	1	67	theme	insulin	170:176	arg1	IR					190:191	IR	190:191	IR	190:191	Obesity-associated insulin resistance (IR) is a major risk factor for developing type 2 diabetes and an array of other metabolic disorders.
29886193	1	67	theme	insulin	170:176	arg1	resistance					178:187	Obesity-associated insulin resistance	151:187	Obesity-associated insulin resistance (IR)	151:192	Obesity-associated insulin resistance (IR) is a major risk factor for developing type 2 diabetes and an array of other metabolic disorders.
29886193	1	67	theme	insulin	170:176	arg1	factor					210:215	a major risk factor	197:215	a major risk factor for developing type 2 diabetes and an array of other metabolic disorders	197:288	Obesity-associated insulin resistance (IR) is a major risk factor for developing type 2 diabetes and an array of other metabolic disorders.
29886193	9	68	theme	PC	1789:1790	arg1	activity					1777:1784	the enzymatic activity	1763:1784	the enzymatic activity of PC	1763:1790	Kaempferol suppression of hepatic gluconeogenesis is due to its direct inhibitory action on the enzymatic activity of PC.
29886193	4	69	theme	obese	884:888	arg1	mice					890:893	obese mice	884:893	obese mice	884:893	Oral administration of kaempferol (50 mg/kg/day), which is the human equivalent dose of 240 mg/day for an average 60 kg human, significantly improved blood glucose control in obese mice, which was associated with reduced hepatic glucose production and improved whole-body insulin sensitivity without altering body weight gain, food consumption or adiposity.
29886193	7	70	theme	PC	1498:1499	arg1	kaempferol					1462:1471	kaempferol	1462:1471	kaempferol	1462:1471	Furthermore, we found that kaempferol is a direct inhibitor of PC.
29886193	7	70	theme	PC	1498:1499	arg1	inhibitor					1485:1493	a direct inhibitor	1476:1493	a direct inhibitor of PC	1476:1499	Furthermore, we found that kaempferol is a direct inhibitor of PC.
29886193	4	71	theme	equivalent	778:787	arg1	dose					789:792	the human equivalent dose	768:792	the human equivalent dose of 240 mg/day for an average 60 kg human	768:833	Oral administration of kaempferol (50 mg/kg/day), which is the human equivalent dose of 240 mg/day for an average 60 kg human, significantly improved blood glucose control in obese mice, which was associated with reduced hepatic glucose production and improved whole-body insulin sensitivity without altering body weight gain, food consumption or adiposity.
29886193	4	71	theme	equivalent	778:787	arg1	administration					714:727	Oral administration	709:727	Oral administration	709:727	Oral administration of kaempferol (50 mg/kg/day), which is the human equivalent dose of 240 mg/day for an average 60 kg human, significantly improved blood glucose control in obese mice, which was associated with reduced hepatic glucose production and improved whole-body insulin sensitivity without altering body weight gain, food consumption or adiposity.
29886193	4	72	theme	kaempferol	732:741	arg1	mg/kg/day					747:755	50 mg/kg/day	744:755	50 mg/kg/day	744:755	Oral administration of kaempferol (50 mg/kg/day), which is the human equivalent dose of 240 mg/day for an average 60 kg human, significantly improved blood glucose control in obese mice, which was associated with reduced hepatic glucose production and improved whole-body insulin sensitivity without altering body weight gain, food consumption or adiposity.
29886193	4	72	theme	kaempferol	732:741	arg1	administration					714:727	Oral administration	709:727	Oral administration	709:727	Oral administration of kaempferol (50 mg/kg/day), which is the human equivalent dose of 240 mg/day for an average 60 kg human, significantly improved blood glucose control in obese mice, which was associated with reduced hepatic glucose production and improved whole-body insulin sensitivity without altering body weight gain, food consumption or adiposity.
29886193	4	72	theme	kaempferol	732:741	arg1	dose					789:792	the human equivalent dose	768:792	the human equivalent dose of 240 mg/day for an average 60 kg human	768:833	Oral administration of kaempferol (50 mg/kg/day), which is the human equivalent dose of 240 mg/day for an average 60 kg human, significantly improved blood glucose control in obese mice, which was associated with reduced hepatic glucose production and improved whole-body insulin sensitivity without altering body weight gain, food consumption or adiposity.
29886193	0	73	theme	hepatic	95:101	arg1	sensitivity					111:121	hepatic insulin sensitivity	95:121	hepatic insulin sensitivity in diet-induced obese mice	95:148	Kaempferol ameliorates hyperglycemia through suppressing hepatic gluconeogenesis and enhancing hepatic insulin sensitivity in diet-induced obese mice.
29886193	3	74	theme	obese	618:622	arg1	mice					624:627	high-fat diet-fed obese mice	600:627	high-fat diet-fed obese mice	600:627	In this study, we explored whether kaempferol, a flavonoid isolated from Gink go biloba, is able to regulate hepatic gluconeogenesis and blood glucose homeostasis in high-fat diet-fed obese mice and further explored the underlying mechanism by which it elicits such effects.
29886193	9	75	from	action	1753:1758	arg1	activity					1777:1784	the enzymatic activity	1763:1784	the enzymatic activity of PC	1763:1790	Kaempferol suppression of hepatic gluconeogenesis is due to its direct inhibitory action on the enzymatic activity of PC.
29886193	3	76	attach	isolated	493:500	arg2	flavonoid					483:491	a flavonoid	481:491	a flavonoid isolated from Gink go biloba	481:520	In this study, we explored whether kaempferol, a flavonoid isolated from Gink go biloba, is able to regulate hepatic gluconeogenesis and blood glucose homeostasis in high-fat diet-fed obese mice and further explored the underlying mechanism by which it elicits such effects.
29886193	3	76	attach	isolated	493:500	arg2	kaempferol					469:478	kaempferol	469:478	kaempferol	469:478	In this study, we explored whether kaempferol, a flavonoid isolated from Gink go biloba, is able to regulate hepatic gluconeogenesis and blood glucose homeostasis in high-fat diet-fed obese mice and further explored the underlying mechanism by which it elicits such effects.
29886193	3	76	attach	isolated	493:500	arg1	Gink					507:510	Gink	507:510	Gink	507:510	In this study, we explored whether kaempferol, a flavonoid isolated from Gink go biloba, is able to regulate hepatic gluconeogenesis and blood glucose homeostasis in high-fat diet-fed obese mice and further explored the underlying mechanism by which it elicits such effects.
29886193	4	77	theme	hepatic	930:936	arg1	production					946:955	reduced hepatic glucose production	922:955	reduced hepatic glucose production	922:955	Oral administration of kaempferol (50 mg/kg/day), which is the human equivalent dose of 240 mg/day for an average 60 kg human, significantly improved blood glucose control in obese mice, which was associated with reduced hepatic glucose production and improved whole-body insulin sensitivity without altering body weight gain, food consumption or adiposity.
29886193	8	78	theme	glucose	1617:1623	arg1	production					1625:1634	glucose production	1617:1634	glucose production	1617:1634	These findings suggest that kaempferol may be a naturally occurring antidiabetic compound that acts by suppressing glucose production and improving insulin sensitivity.
31325218	8	0	theme	LL-PAP	1396:1401	arg1	effects					1385:1391	no protective effects	1371:1391	no protective effects of LL-PAP	1371:1401	After the DSS challenge, no protective effects of LL-PAP could be detected.
31325218	4	1	theme	epithelial	787:796	arg1	inflammation					798:809	epithelial inflammation	787:809	epithelial inflammation	787:809	PAP protein plays a protective effect presenting anti-inflammatory properties able to reduce the severity of colitis, preserving gut barrier and epithelial inflammation.
31325218	10	2	from	severity	1658:1665	arg1	model					1705:1709	DNBS colitis model	1692:1709	DNBS colitis model	1692:1709	Based on our findings, we hypothesize that a treatment with LL-PAP shifts the microbiota preventing the severity of colon inflammation in DNBS colitis model.
31325218	1	3	theme	important	290:298	arg1	effectors					300:308	important effectors	290:308	important effectors of innate immunity	290:327	Antimicrobial peptides secreted by intestinal immune and epithelial cells are important effectors of innate immunity.
31325218	1	3	theme	important	290:298	arg1	peptides					226:233	Antimicrobial peptides	212:233	Antimicrobial peptides secreted by intestinal immune and epithelial cells	212:284	Antimicrobial peptides secreted by intestinal immune and epithelial cells are important effectors of innate immunity.
31325218	0	4	theme	microbiota	200:209	arg1	composition					181:191	the composition	177:191	the composition of the microbiota	177:209	Oral delivery of pancreatitis-associated protein by Lactococcus lactis displays protective effects in dinitro-benzenesulfonic-acid-induced colitis model and is able to modulate the composition of the microbiota.
31325218	2	5	theme	homeostasis	391:401	arg1	maintenance					365:375	the maintenance	361:375	the maintenance of intestinal homeostasis	361:401	They play an essential role in the maintenance of intestinal homeostasis by limiting microbial epithelium interactions and preventing unnecessary microbe-driven inflammation.
31325218	10	6	with	treatment	1599:1607	arg1	LL-PAP					1614:1619	LL-PAP	1614:1619	LL-PAP	1614:1619	Based on our findings, we hypothesize that a treatment with LL-PAP shifts the microbiota preventing the severity of colon inflammation in DNBS colitis model.
31325218	6	7	theme	recombinant	1081:1091	arg1	strains					1093:1099	our recombinant strains	1077:1099	our recombinant strains	1077:1099	After construction and characterization of our recombinant strains, we tested their effects in dinitro-benzenesulfonic-acid (DNBS) and Dextran sulfate sodium (DSS) colitis model.
31325218	0	8	theme	colitis	139:145	arg1	model					147:151	dinitro-benzenesulfonic-acid-induced colitis model	102:151	dinitro-benzenesulfonic-acid-induced colitis model	102:151	Oral delivery of pancreatitis-associated protein by Lactococcus lactis displays protective effects in dinitro-benzenesulfonic-acid-induced colitis model and is able to modulate the composition of the microbiota.
31325218	11	9	theme	protective	1718:1727	arg1	roles					1729:1733	These protective roles	1712:1733	These protective roles of LL-PAP in DNBS colitis model	1712:1765	These protective roles of LL-PAP in DNBS colitis model might be through intestinal microbiota modulation.
31325218	3	10	theme	dependent	623:631	arg1	Ca+2					618:621	Ca+2 dependent	618:631	Ca+2 dependent	618:631	Pancreatitis-associated protein (PAP) belongs to Regenerating islet-derived III proteins family and is a C-type (Ca+2 dependent) lectin.
31325218	3	10	theme	dependent	623:631	arg1	lectin					634:639	a C-type (Ca+2 dependent) lectin	608:639	a C-type (Ca+2 dependent) lectin	608:639	Pancreatitis-associated protein (PAP) belongs to Regenerating islet-derived III proteins family and is a C-type (Ca+2 dependent) lectin.
31325218	10	11	theme	DNBS	1692:1695	arg1	model					1705:1709	DNBS colitis model	1692:1709	DNBS colitis model	1692:1709	Based on our findings, we hypothesize that a treatment with LL-PAP shifts the microbiota preventing the severity of colon inflammation in DNBS colitis model.
31325218	0	12	theme	dinitro-benzenesulfonic-acid-induced	102:137	arg1	model					147:151	dinitro-benzenesulfonic-acid-induced colitis model	102:151	dinitro-benzenesulfonic-acid-induced colitis model	102:151	Oral delivery of pancreatitis-associated protein by Lactococcus lactis displays protective effects in dinitro-benzenesulfonic-acid-induced colitis model and is able to modulate the composition of the microbiota.
31325218	11	13	theme	DNBS	1748:1751	arg1	model					1761:1765	DNBS colitis model	1748:1765	DNBS colitis model	1748:1765	These protective roles of LL-PAP in DNBS colitis model might be through intestinal microbiota modulation.
31325218	7	14	theme	LL-empty-treated	1316:1331	arg1	groups					1338:1343	LL-empty-treated mice groups	1316:1343	LL-empty-treated mice groups	1316:1343	After the DNBS challenge, mice treated with LL-PAP presented less severe colitis compared with PBS and LL-empty-treated mice groups.
31325218	6	15	theme	sodium	1185:1190	arg1	model					1206:1210	Dextran sulfate sodium (DSS) colitis model	1169:1210	Dextran sulfate sodium (DSS) colitis model	1169:1210	After construction and characterization of our recombinant strains, we tested their effects in dinitro-benzenesulfonic-acid (DNBS) and Dextran sulfate sodium (DSS) colitis model.
31325218	1	16	theme	innate	313:318	arg1	immunity					320:327	innate immunity	313:327	innate immunity	313:327	Antimicrobial peptides secreted by intestinal immune and epithelial cells are important effectors of innate immunity.
31325218	5	17	theme	recombinant	886:896	arg1	LL-PAP					925:930	LL-PAP	925:930	LL-PAP	925:930	Here, we sought to determine whether PAP delivered at intestinal lumen by recombinant Lactococcus lactis strain (LL-PAP) before and after chemically induced colitis is able to reduce the severity in two models of colitis.
31325218	5	17	theme	recombinant	886:896	arg1	strain					917:922	recombinant Lactococcus lactis strain	886:922	recombinant Lactococcus lactis strain (LL-PAP)	886:931	Here, we sought to determine whether PAP delivered at intestinal lumen by recombinant Lactococcus lactis strain (LL-PAP) before and after chemically induced colitis is able to reduce the severity in two models of colitis.
31325218	3	18	theme	proteins	585:592	arg1	family					594:599	Regenerating islet-derived III proteins family	554:599	Regenerating islet-derived III proteins family	554:599	Pancreatitis-associated protein (PAP) belongs to Regenerating islet-derived III proteins family and is a C-type (Ca+2 dependent) lectin.
31325218	6	19	theme	Dextran	1169:1175	arg1	DSS					1193:1195	DSS	1193:1195	DSS	1193:1195	After construction and characterization of our recombinant strains, we tested their effects in dinitro-benzenesulfonic-acid (DNBS) and Dextran sulfate sodium (DSS) colitis model.
31325218	6	19	theme	Dextran	1169:1175	arg1	sodium					1185:1190	Dextran sulfate sodium	1169:1190	Dextran sulfate sodium (DSS) colitis model	1169:1210	After construction and characterization of our recombinant strains, we tested their effects in dinitro-benzenesulfonic-acid (DNBS) and Dextran sulfate sodium (DSS) colitis model.
31325218	3	20	theme	Pancreatitis-associated	505:527	arg1	PAP					538:540	PAP	538:540	PAP	538:540	Pancreatitis-associated protein (PAP) belongs to Regenerating islet-derived III proteins family and is a C-type (Ca+2 dependent) lectin.
31325218	3	20	theme	Pancreatitis-associated	505:527	arg1	protein					529:535	Pancreatitis-associated protein	505:535	Pancreatitis-associated protein (PAP)	505:541	Pancreatitis-associated protein (PAP) belongs to Regenerating islet-derived III proteins family and is a C-type (Ca+2 dependent) lectin.
31325218	4	21	theme	colitis	751:757	arg1	severity					739:746	the severity	735:746	the severity of colitis	735:757	PAP protein plays a protective effect presenting anti-inflammatory properties able to reduce the severity of colitis, preserving gut barrier and epithelial inflammation.
31325218	4	22	theme	protective	662:671	arg1	effect					673:678	a protective effect	660:678	a protective effect	660:678	PAP protein plays a protective effect presenting anti-inflammatory properties able to reduce the severity of colitis, preserving gut barrier and epithelial inflammation.
31325218	11	23	theme	LL-PAP	1738:1743	arg1	roles					1729:1733	These protective roles	1712:1733	These protective roles of LL-PAP in DNBS colitis model	1712:1765	These protective roles of LL-PAP in DNBS colitis model might be through intestinal microbiota modulation.
31325218	6	24	theme	colitis	1198:1204	arg1	model					1206:1210	Dextran sulfate sodium (DSS) colitis model	1169:1210	Dextran sulfate sodium (DSS) colitis model	1169:1210	After construction and characterization of our recombinant strains, we tested their effects in dinitro-benzenesulfonic-acid (DNBS) and Dextran sulfate sodium (DSS) colitis model.
31325218	5	25	theme	lactis	910:915	arg1	LL-PAP					925:930	LL-PAP	925:930	LL-PAP	925:930	Here, we sought to determine whether PAP delivered at intestinal lumen by recombinant Lactococcus lactis strain (LL-PAP) before and after chemically induced colitis is able to reduce the severity in two models of colitis.
31325218	5	25	theme	lactis	910:915	arg1	strain					917:922	recombinant Lactococcus lactis strain	886:922	recombinant Lactococcus lactis strain (LL-PAP)	886:931	Here, we sought to determine whether PAP delivered at intestinal lumen by recombinant Lactococcus lactis strain (LL-PAP) before and after chemically induced colitis is able to reduce the severity in two models of colitis.
31325218	5	26	theme	Lactococcus	898:908	arg1	LL-PAP					925:930	LL-PAP	925:930	LL-PAP	925:930	Here, we sought to determine whether PAP delivered at intestinal lumen by recombinant Lactococcus lactis strain (LL-PAP) before and after chemically induced colitis is able to reduce the severity in two models of colitis.
31325218	5	26	theme	Lactococcus	898:908	arg1	strain					917:922	recombinant Lactococcus lactis strain	886:922	recombinant Lactococcus lactis strain (LL-PAP)	886:931	Here, we sought to determine whether PAP delivered at intestinal lumen by recombinant Lactococcus lactis strain (LL-PAP) before and after chemically induced colitis is able to reduce the severity in two models of colitis.
31325218	10	27	theme	inflammation	1676:1687	arg1	severity					1658:1665	the severity	1654:1665	the severity of colon inflammation in DNBS colitis model	1654:1709	Based on our findings, we hypothesize that a treatment with LL-PAP shifts the microbiota preventing the severity of colon inflammation in DNBS colitis model.
31325218	6	28	from	effects	1118:1124	arg1	dinitro-benzenesulfonic-acid					1129:1156	dinitro-benzenesulfonic-acid	1129:1156	dinitro-benzenesulfonic-acid (DNBS)	1129:1163	After construction and characterization of our recombinant strains, we tested their effects in dinitro-benzenesulfonic-acid (DNBS) and Dextran sulfate sodium (DSS) colitis model.
31325218	6	28	from	effects	1118:1124	arg1	DNBS					1159:1162	DNBS	1159:1162	DNBS	1159:1162	After construction and characterization of our recombinant strains, we tested their effects in dinitro-benzenesulfonic-acid (DNBS) and Dextran sulfate sodium (DSS) colitis model.
31325218	11	29	theme	colitis	1753:1759	arg1	model					1761:1765	DNBS colitis model	1748:1765	DNBS colitis model	1748:1765	These protective roles of LL-PAP in DNBS colitis model might be through intestinal microbiota modulation.
31325218	1	30	theme	Antimicrobial	212:224	arg1	effectors					300:308	important effectors	290:308	important effectors of innate immunity	290:327	Antimicrobial peptides secreted by intestinal immune and epithelial cells are important effectors of innate immunity.
31325218	1	30	theme	Antimicrobial	212:224	arg1	peptides					226:233	Antimicrobial peptides	212:233	Antimicrobial peptides secreted by intestinal immune and epithelial cells	212:284	Antimicrobial peptides secreted by intestinal immune and epithelial cells are important effectors of innate immunity.
31325218	0	31	theme	Oral	0:3	arg1	delivery					5:12	Oral delivery	0:12	Oral delivery of pancreatitis-associated protein by Lactococcus lactis	0:69	Oral delivery of pancreatitis-associated protein by Lactococcus lactis displays protective effects in dinitro-benzenesulfonic-acid-induced colitis model and is able to modulate the composition of the microbiota.
31325218	1	32	theme	immunity	320:327	arg1	effectors					300:308	important effectors	290:308	important effectors of innate immunity	290:327	Antimicrobial peptides secreted by intestinal immune and epithelial cells are important effectors of innate immunity.
31325218	1	32	theme	immunity	320:327	arg1	peptides					226:233	Antimicrobial peptides	212:233	Antimicrobial peptides secreted by intestinal immune and epithelial cells	212:284	Antimicrobial peptides secreted by intestinal immune and epithelial cells are important effectors of innate immunity.
31325218	7	33	theme	DNBS	1223:1226	arg1	challenge					1228:1236	the DNBS challenge	1219:1236	the DNBS challenge	1219:1236	After the DNBS challenge, mice treated with LL-PAP presented less severe colitis compared with PBS and LL-empty-treated mice groups.
31325218	6	34	theme	strains	1093:1099	arg1	construction					1040:1051	construction	1040:1051	construction	1040:1051	After construction and characterization of our recombinant strains, we tested their effects in dinitro-benzenesulfonic-acid (DNBS) and Dextran sulfate sodium (DSS) colitis model.
31325218	6	34	theme	strains	1093:1099	arg1	characterization					1057:1072	characterization	1057:1072	characterization	1057:1072	After construction and characterization of our recombinant strains, we tested their effects in dinitro-benzenesulfonic-acid (DNBS) and Dextran sulfate sodium (DSS) colitis model.
31325218	2	35	theme	epithelium	425:434	arg1	interactions					436:447	microbial epithelium interactions	415:447	microbial epithelium interactions	415:447	They play an essential role in the maintenance of intestinal homeostasis by limiting microbial epithelium interactions and preventing unnecessary microbe-driven inflammation.
31325218	0	36	theme	pancreatitis-associated	17:39	arg1	protein					41:47	pancreatitis-associated protein	17:47	pancreatitis-associated protein	17:47	Oral delivery of pancreatitis-associated protein by Lactococcus lactis displays protective effects in dinitro-benzenesulfonic-acid-induced colitis model and is able to modulate the composition of the microbiota.
31325218	8	37	theme	protective	1374:1383	arg1	effects					1385:1391	no protective effects	1371:1391	no protective effects of LL-PAP	1371:1401	After the DSS challenge, no protective effects of LL-PAP could be detected.
31325218	11	38	theme	intestinal	1784:1793	arg1	modulation					1806:1815	intestinal microbiota modulation	1784:1815	intestinal microbiota modulation	1784:1815	These protective roles of LL-PAP in DNBS colitis model might be through intestinal microbiota modulation.
31325218	5	39	from	severity	999:1006	arg1	models					1015:1020	two models	1011:1020	two models of colitis	1011:1031	Here, we sought to determine whether PAP delivered at intestinal lumen by recombinant Lactococcus lactis strain (LL-PAP) before and after chemically induced colitis is able to reduce the severity in two models of colitis.
31325218	2	40	theme	essential	343:351	arg1	role					353:356	an essential role	340:356	an essential role	340:356	They play an essential role in the maintenance of intestinal homeostasis by limiting microbial epithelium interactions and preventing unnecessary microbe-driven inflammation.
31325218	9	41	theme	days	1449:1452	arg1	administration					1454:1467	5 days administration	1447:1467	5 days administration	1447:1467	We determined that after 5 days administration, LL-PAP increase butyrate producer's bacteria, especially Eubacterium plexicaudatum.
31325218	6	42	theme	sulfate	1177:1183	arg1	DSS					1193:1195	DSS	1193:1195	DSS	1193:1195	After construction and characterization of our recombinant strains, we tested their effects in dinitro-benzenesulfonic-acid (DNBS) and Dextran sulfate sodium (DSS) colitis model.
31325218	6	42	theme	sulfate	1177:1183	arg1	sodium					1185:1190	Dextran sulfate sodium	1169:1190	Dextran sulfate sodium (DSS) colitis model	1169:1210	After construction and characterization of our recombinant strains, we tested their effects in dinitro-benzenesulfonic-acid (DNBS) and Dextran sulfate sodium (DSS) colitis model.
31325218	3	43	theme	Regenerating	554:565	arg1	family					594:599	Regenerating islet-derived III proteins family	554:599	Regenerating islet-derived III proteins family	554:599	Pancreatitis-associated protein (PAP) belongs to Regenerating islet-derived III proteins family and is a C-type (Ca+2 dependent) lectin.
31325218	5	44	theme	intestinal	866:875	arg1	lumen					877:881	intestinal lumen	866:881	intestinal lumen	866:881	Here, we sought to determine whether PAP delivered at intestinal lumen by recombinant Lactococcus lactis strain (LL-PAP) before and after chemically induced colitis is able to reduce the severity in two models of colitis.
31325218	4	45	theme	PAP	642:644	arg1	protein					646:652	PAP protein	642:652	PAP protein	642:652	PAP protein plays a protective effect presenting anti-inflammatory properties able to reduce the severity of colitis, preserving gut barrier and epithelial inflammation.
31325218	1	46	theme	intestinal	247:256	arg1	cells					280:284	intestinal immune and epithelial cells	247:284	intestinal immune and epithelial cells	247:284	Antimicrobial peptides secreted by intestinal immune and epithelial cells are important effectors of innate immunity.
31325218	11	47	theme	microbiota	1795:1804	arg1	modulation					1806:1815	intestinal microbiota modulation	1784:1815	intestinal microbiota modulation	1784:1815	These protective roles of LL-PAP in DNBS colitis model might be through intestinal microbiota modulation.
31325218	2	48	theme	microbe-driven	476:489	arg1	inflammation					491:502	unnecessary microbe-driven inflammation	464:502	unnecessary microbe-driven inflammation	464:502	They play an essential role in the maintenance of intestinal homeostasis by limiting microbial epithelium interactions and preventing unnecessary microbe-driven inflammation.
31325218	0	49	theme	protein	41:47	arg1	delivery					5:12	Oral delivery	0:12	Oral delivery of pancreatitis-associated protein by Lactococcus lactis	0:69	Oral delivery of pancreatitis-associated protein by Lactococcus lactis displays protective effects in dinitro-benzenesulfonic-acid-induced colitis model and is able to modulate the composition of the microbiota.
31325218	7	50	theme	mice	1333:1336	arg1	groups					1338:1343	LL-empty-treated mice groups	1316:1343	LL-empty-treated mice groups	1316:1343	After the DNBS challenge, mice treated with LL-PAP presented less severe colitis compared with PBS and LL-empty-treated mice groups.
31325218	5	51	theme	induced	961:967	arg1	colitis					969:975	chemically induced colitis	950:975	chemically induced colitis	950:975	Here, we sought to determine whether PAP delivered at intestinal lumen by recombinant Lactococcus lactis strain (LL-PAP) before and after chemically induced colitis is able to reduce the severity in two models of colitis.
31325218	8	52	theme	DSS	1356:1358	arg1	challenge					1360:1368	the DSS challenge	1352:1368	the DSS challenge	1352:1368	After the DSS challenge, no protective effects of LL-PAP could be detected.
31325218	10	53	theme	colon	1670:1674	arg1	inflammation					1676:1687	colon inflammation	1670:1687	colon inflammation	1670:1687	Based on our findings, we hypothesize that a treatment with LL-PAP shifts the microbiota preventing the severity of colon inflammation in DNBS colitis model.
31325218	1	54	theme	immune	258:263	arg1	cells					280:284	intestinal immune and epithelial cells	247:284	intestinal immune and epithelial cells	247:284	Antimicrobial peptides secreted by intestinal immune and epithelial cells are important effectors of innate immunity.
31325218	10	55	theme	colitis	1697:1703	arg1	model					1705:1709	DNBS colitis model	1692:1709	DNBS colitis model	1692:1709	Based on our findings, we hypothesize that a treatment with LL-PAP shifts the microbiota preventing the severity of colon inflammation in DNBS colitis model.
31325218	3	56	link	islet-derived	567:579	arg1	family					594:599	Regenerating islet-derived III proteins family	554:599	Regenerating islet-derived III proteins family	554:599	Pancreatitis-associated protein (PAP) belongs to Regenerating islet-derived III proteins family and is a C-type (Ca+2 dependent) lectin.
31325218	2	57	theme	intestinal	380:389	arg1	homeostasis					391:401	intestinal homeostasis	380:401	intestinal homeostasis	380:401	They play an essential role in the maintenance of intestinal homeostasis by limiting microbial epithelium interactions and preventing unnecessary microbe-driven inflammation.
31325218	0	58	theme	Lactococcus	52:62	arg1	lactis					64:69	Lactococcus lactis	52:69	Lactococcus lactis	52:69	Oral delivery of pancreatitis-associated protein by Lactococcus lactis displays protective effects in dinitro-benzenesulfonic-acid-induced colitis model and is able to modulate the composition of the microbiota.
31325218	6	59	from	model	1206:1210	arg1	dinitro-benzenesulfonic-acid					1129:1156	dinitro-benzenesulfonic-acid	1129:1156	dinitro-benzenesulfonic-acid (DNBS)	1129:1163	After construction and characterization of our recombinant strains, we tested their effects in dinitro-benzenesulfonic-acid (DNBS) and Dextran sulfate sodium (DSS) colitis model.
31325218	6	59	from	model	1206:1210	arg1	DNBS					1159:1162	DNBS	1159:1162	DNBS	1159:1162	After construction and characterization of our recombinant strains, we tested their effects in dinitro-benzenesulfonic-acid (DNBS) and Dextran sulfate sodium (DSS) colitis model.
31325218	3	60	theme	islet-derived	567:579	arg1	family					594:599	Regenerating islet-derived III proteins family	554:599	Regenerating islet-derived III proteins family	554:599	Pancreatitis-associated protein (PAP) belongs to Regenerating islet-derived III proteins family and is a C-type (Ca+2 dependent) lectin.
31325218	5	61	theme	colitis	1025:1031	arg1	models					1015:1020	two models	1011:1020	two models of colitis	1011:1031	Here, we sought to determine whether PAP delivered at intestinal lumen by recombinant Lactococcus lactis strain (LL-PAP) before and after chemically induced colitis is able to reduce the severity in two models of colitis.
31325218	4	62	theme	anti-inflammatory	691:707	arg1	properties					709:718	anti-inflammatory properties	691:718	anti-inflammatory properties able to reduce the severity of colitis	691:757	PAP protein plays a protective effect presenting anti-inflammatory properties able to reduce the severity of colitis, preserving gut barrier and epithelial inflammation.
31325218	9	63	theme	butyrate	1486:1493	arg1	bacteria					1506:1513	butyrate producer's bacteria	1486:1513	butyrate producer's bacteria	1486:1513	We determined that after 5 days administration, LL-PAP increase butyrate producer's bacteria, especially Eubacterium plexicaudatum.
31325218	9	63	theme	butyrate	1486:1493	arg1	plexicaudatum					1539:1551	Eubacterium plexicaudatum	1527:1551	Eubacterium plexicaudatum	1527:1551	We determined that after 5 days administration, LL-PAP increase butyrate producer's bacteria, especially Eubacterium plexicaudatum.
31325218	4	64	theme	gut	771:773	arg1	barrier					775:781	gut barrier	771:781	gut barrier	771:781	PAP protein plays a protective effect presenting anti-inflammatory properties able to reduce the severity of colitis, preserving gut barrier and epithelial inflammation.
31325218	1	65	theme	epithelial	269:278	arg1	cells					280:284	intestinal immune and epithelial cells	247:284	intestinal immune and epithelial cells	247:284	Antimicrobial peptides secreted by intestinal immune and epithelial cells are important effectors of innate immunity.
31325218	0	66	theme	protective	80:89	arg1	effects					91:97	protective effects	80:97	protective effects	80:97	Oral delivery of pancreatitis-associated protein by Lactococcus lactis displays protective effects in dinitro-benzenesulfonic-acid-induced colitis model and is able to modulate the composition of the microbiota.
31325218	2	67	theme	unnecessary	464:474	arg1	inflammation					491:502	unnecessary microbe-driven inflammation	464:502	unnecessary microbe-driven inflammation	464:502	They play an essential role in the maintenance of intestinal homeostasis by limiting microbial epithelium interactions and preventing unnecessary microbe-driven inflammation.
31325218	2	68	theme	microbial	415:423	arg1	interactions					436:447	microbial epithelium interactions	415:447	microbial epithelium interactions	415:447	They play an essential role in the maintenance of intestinal homeostasis by limiting microbial epithelium interactions and preventing unnecessary microbe-driven inflammation.
31325218	4	69	theme	able	720:723	arg1	properties					709:718	anti-inflammatory properties	691:718	anti-inflammatory properties able to reduce the severity of colitis	691:757	PAP protein plays a protective effect presenting anti-inflammatory properties able to reduce the severity of colitis, preserving gut barrier and epithelial inflammation.
31325218	11	70	from	roles	1729:1733	arg1	model					1761:1765	DNBS colitis model	1748:1765	DNBS colitis model	1748:1765	These protective roles of LL-PAP in DNBS colitis model might be through intestinal microbiota modulation.
31325218	3	71	theme	C-type	610:615	arg1	Ca+2					618:621	Ca+2 dependent	618:631	Ca+2 dependent	618:631	Pancreatitis-associated protein (PAP) belongs to Regenerating islet-derived III proteins family and is a C-type (Ca+2 dependent) lectin.
31325218	3	71	theme	C-type	610:615	arg1	lectin					634:639	a C-type (Ca+2 dependent) lectin	608:639	a C-type (Ca+2 dependent) lectin	608:639	Pancreatitis-associated protein (PAP) belongs to Regenerating islet-derived III proteins family and is a C-type (Ca+2 dependent) lectin.
31325218	7	72	theme	severe	1279:1284	arg1	colitis					1286:1292	less severe colitis	1274:1292	less severe colitis	1274:1292	After the DNBS challenge, mice treated with LL-PAP presented less severe colitis compared with PBS and LL-empty-treated mice groups.
30820713	12	0	theme	 = CCUG	1732:1738	arg1	B-65515T					1723:1730	= NRRL B-65515T	1716:1730	= NRRL B-65515T	1716:1730	nov. is proposed, with type strain PB300T (= NRRL B-65515T, = CCUG 72241T).
30820713	12	0	theme	 = CCUG	1732:1738	arg1	72241T					1740:1745	 = CCUG 72241T	1732:1745	 = CCUG 72241T	1732:1745	nov. is proposed, with type strain PB300T (= NRRL B-65515T, = CCUG 72241T).
30820713	9	1	theme	strain	1121:1126	arg1	genome					1103:1108	the core genome	1094:1108	the core genome of the new strain and 5 closely related type strains of the genus Lysinibacillus	1094:1189	A phylogenomic analysis of the core genome of the new strain and 5 closely related type strains of the genus Lysinibacillus revealed that this strain formed a distinct monophyletic clade with the nearest neighbour being Lysinibacillus boronitolerans.
30820713	12	2	theme	strain	1701:1706	arg1	PB300T					1708:1713	type strain PB300T	1696:1713	type strain PB300T (= NRRL B-65515T, = CCUG 72241T)	1696:1746	nov. is proposed, with type strain PB300T (= NRRL B-65515T, = CCUG 72241T).
30820713	5	3	with	media	685:689	arg1	pH					726:727	optimal growth at pH 7.0	708:731	optimal growth at pH 7.0	708:731	Furthermore, the strain was found to grow in media with pH 5 to 10 (optimal growth at pH 7.0).
30820713	5	3	with	media	685:689	arg1	pH					696:697	pH 5 to 10	696:705	pH 5 to 10 (optimal growth at pH 7.0)	696:732	Furthermore, the strain was found to grow in media with pH 5 to 10 (optimal growth at pH 7.0).
30820713	10	4	theme	species	1446:1452	arg1	threshold					1454:1462	the species threshold	1442:1462	the species threshold of 70%	1442:1469	DNA-DNA relatedness studies using in silico DNA-DNA hybridizations (DDH) showed relationships for the new strain were below the species threshold of 70%.
30820713	6	5	theme	cellular	751:758	arg1	iso-C15 					792:799	iso-C15 	792:799	iso-C15 	792:799	The predominant cellular fatty acids were observed to be iso-C15 : 0 (56.6 %), anteiso-C15 : 0 (14.6%), C16 :1ω7C alcohol (9.3%) and C16 : 0 (7.1%).
30820713	6	5	theme	cellular	751:758	arg1	acids					766:770	The predominant cellular fatty acids	735:770	The predominant cellular fatty acids	735:770	The predominant cellular fatty acids were observed to be iso-C15 : 0 (56.6 %), anteiso-C15 : 0 (14.6%), C16 :1ω7C alcohol (9.3%) and C16 : 0 (7.1%).
30820713	2	6	theme	plant	235:239	arg1	soil					218:221	rhizospheric soil	205:221	rhizospheric soil of a pepper plant when screening and bioprospecting for plant beneficial microorganisms	205:309	A strain of a Gram-positive, strictly aerobic, motile, rod-shaped, endospore forming bacterium was originally isolated from rhizospheric soil of a pepper plant when screening and bioprospecting for plant beneficial microorganisms.
30820713	9	7	theme	related	1142:1148	arg1	strains					1155:1161	5 closely related type strains	1132:1161	5 closely related type strains of the genus Lysinibacillus	1132:1189	A phylogenomic analysis of the core genome of the new strain and 5 closely related type strains of the genus Lysinibacillus revealed that this strain formed a distinct monophyletic clade with the nearest neighbour being Lysinibacillus boronitolerans.
30820713	10	8	theme	relatedness	1326:1336	arg1	studies					1338:1344	DNA-DNA relatedness studies	1318:1344	DNA-DNA relatedness studies using in silico DNA-DNA hybridizations (DDH)	1318:1389	DNA-DNA relatedness studies using in silico DNA-DNA hybridizations (DDH) showed relationships for the new strain were below the species threshold of 70%.
30820713	11	9	theme	phenotypic	1517:1526	arg1	analyses					1528:1535	phylogenetic and phenotypic analyses	1500:1535	phylogenetic and phenotypic analyses	1500:1535	Based upon the consensus of phylogenetic and phenotypic analyses, we conclude that this strain represents a novel species within the genus Lysinibacillus, for which the name Lysinibacillus capsici sp.
30820713	2	10	theme	plant	279:283	arg1	microorganisms					296:309	plant beneficial microorganisms	279:309	plant beneficial microorganisms	279:309	A strain of a Gram-positive, strictly aerobic, motile, rod-shaped, endospore forming bacterium was originally isolated from rhizospheric soil of a pepper plant when screening and bioprospecting for plant beneficial microorganisms.
30820713	9	11	theme	strains	1155:1161	arg1	genome					1103:1108	the core genome	1094:1108	the core genome of the new strain and 5 closely related type strains of the genus Lysinibacillus	1094:1189	A phylogenomic analysis of the core genome of the new strain and 5 closely related type strains of the genus Lysinibacillus revealed that this strain formed a distinct monophyletic clade with the nearest neighbour being Lysinibacillus boronitolerans.
30820713	8	12	theme	DNA	1007:1009	arg1	%					1050:1050	37.5%	1046:1050	37.5% (mol content)	1046:1064	A draft genome was completed and the DNA G + C content was determined to be 37.5% (mol content).
30820713	8	12	theme	DNA	1007:1009	arg1	content					1017:1023	the DNA G + C content	1003:1023	the DNA G + C content	1003:1023	A draft genome was completed and the DNA G + C content was determined to be 37.5% (mol content).
30820713	11	13	theme	phylogenetic	1500:1511	arg1	analyses					1528:1535	phylogenetic and phenotypic analyses	1500:1535	phylogenetic and phenotypic analyses	1500:1535	Based upon the consensus of phylogenetic and phenotypic analyses, we conclude that this strain represents a novel species within the genus Lysinibacillus, for which the name Lysinibacillus capsici sp.
30820713	5	14	theme	optimal	708:714	arg1	pH					696:697	pH 5 to 10	696:705	pH 5 to 10 (optimal growth at pH 7.0)	696:732	Furthermore, the strain was found to grow in media with pH 5 to 10 (optimal growth at pH 7.0).
30820713	5	14	theme	optimal	708:714	arg1	pH					726:727	optimal growth at pH 7.0	708:731	optimal growth at pH 7.0	708:731	Furthermore, the strain was found to grow in media with pH 5 to 10 (optimal growth at pH 7.0).
30820713	9	15	theme	Lysinibacillus	1176:1189	arg1	strain					1121:1126	the new strain	1113:1126	the new strain	1113:1126	A phylogenomic analysis of the core genome of the new strain and 5 closely related type strains of the genus Lysinibacillus revealed that this strain formed a distinct monophyletic clade with the nearest neighbour being Lysinibacillus boronitolerans.
30820713	9	15	theme	Lysinibacillus	1176:1189	arg1	strains					1155:1161	5 closely related type strains	1132:1161	5 closely related type strains of the genus Lysinibacillus	1132:1189	A phylogenomic analysis of the core genome of the new strain and 5 closely related type strains of the genus Lysinibacillus revealed that this strain formed a distinct monophyletic clade with the nearest neighbour being Lysinibacillus boronitolerans.
30820713	8	16	theme	draft	972:976	arg1	genome					978:983	A draft genome	970:983	A draft genome	970:983	A draft genome was completed and the DNA G + C content was determined to be 37.5% (mol content).
30820713	9	17	theme	Lysinibacillus	1287:1300	arg1	neighbour					1271:1279	the nearest neighbour	1259:1279	the nearest neighbour	1259:1279	A phylogenomic analysis of the core genome of the new strain and 5 closely related type strains of the genus Lysinibacillus revealed that this strain formed a distinct monophyletic clade with the nearest neighbour being Lysinibacillus boronitolerans.
30820713	9	17	theme	Lysinibacillus	1287:1300	arg1	boronitolerans					1302:1315	Lysinibacillus boronitolerans	1287:1315	Lysinibacillus boronitolerans	1287:1315	A phylogenomic analysis of the core genome of the new strain and 5 closely related type strains of the genus Lysinibacillus revealed that this strain formed a distinct monophyletic clade with the nearest neighbour being Lysinibacillus boronitolerans.
30820713	3	18	theme	Phylogenetic	312:323	arg1	analysis					325:332	Phylogenetic analysis	312:332	Phylogenetic analysis of the 16S rRNA gene sequences	312:363	Phylogenetic analysis of the 16S rRNA gene sequences indicated that this strain, PB300T, is closely related to Lysinibacillus macroides DMS 54T (99.6%) and Lysinibacillus xylanilyticus DSM 23493T (99.4%).
30820713	11	19	theme	name	1641:1644	arg1	sp					1669:1670	the name Lysinibacillus capsici sp	1637:1670	the name Lysinibacillus capsici sp	1637:1670	Based upon the consensus of phylogenetic and phenotypic analyses, we conclude that this strain represents a novel species within the genus Lysinibacillus, for which the name Lysinibacillus capsici sp.
30820713	5	20	from	pH	726:727	arg1	growth					716:721	optimal growth at pH 7.0	708:731	optimal growth at pH 7.0	708:731	Furthermore, the strain was found to grow in media with pH 5 to 10 (optimal growth at pH 7.0).
30820713	5	20	from	pH	726:727	arg1	at					723:724	optimal growth at pH 7.0	708:731	optimal growth at pH 7.0	708:731	Furthermore, the strain was found to grow in media with pH 5 to 10 (optimal growth at pH 7.0).
30820713	4	21	theme	%	626:626	arg1	NaCl					634:637	10% (w/v) NaCl	624:637	10% (w/v) NaCl	624:637	In phenotypic characterisation, the novel strain was found to grow between 15 and 40 °C and tolerate up to 10% (w/v) NaCl.
30820713	9	22	theme	monophyletic	1235:1246	arg1	clade					1248:1252	a distinct monophyletic clade	1224:1252	a distinct monophyletic clade	1224:1252	A phylogenomic analysis of the core genome of the new strain and 5 closely related type strains of the genus Lysinibacillus revealed that this strain formed a distinct monophyletic clade with the nearest neighbour being Lysinibacillus boronitolerans.
30820713	3	23	theme	16S	341:343	arg1	sequences					355:363	the 16S rRNA gene sequences	337:363	the 16S rRNA gene sequences	337:363	Phylogenetic analysis of the 16S rRNA gene sequences indicated that this strain, PB300T, is closely related to Lysinibacillus macroides DMS 54T (99.6%) and Lysinibacillus xylanilyticus DSM 23493T (99.4%).
30820713	7	24	theme	close	953:957	arg1	relatives					959:967	its close relatives	949:967	its close relatives	949:967	The cell wall peptidoglycan contains lysine-aspartic acid, as in its close relatives.
30820713	2	25	attach	isolated	191:198	arg2	strain					83:88	A strain	81:88	A strain of a Gram-positive, strictly aerobic, motile, rod-shaped, endospore forming bacterium	81:174	A strain of a Gram-positive, strictly aerobic, motile, rod-shaped, endospore forming bacterium was originally isolated from rhizospheric soil of a pepper plant when screening and bioprospecting for plant beneficial microorganisms.
30820713	2	25	attach	isolated	191:198	arg1	soil					218:221	rhizospheric soil	205:221	rhizospheric soil of a pepper plant when screening and bioprospecting for plant beneficial microorganisms	205:309	A strain of a Gram-positive, strictly aerobic, motile, rod-shaped, endospore forming bacterium was originally isolated from rhizospheric soil of a pepper plant when screening and bioprospecting for plant beneficial microorganisms.
30820713	11	26	theme	Lysinibacillus	1646:1659	arg1	sp					1669:1670	the name Lysinibacillus capsici sp	1637:1670	the name Lysinibacillus capsici sp	1637:1670	Based upon the consensus of phylogenetic and phenotypic analyses, we conclude that this strain represents a novel species within the genus Lysinibacillus, for which the name Lysinibacillus capsici sp.
30820713	9	27	theme	nearest	1263:1269	arg1	neighbour					1271:1279	the nearest neighbour	1259:1279	the nearest neighbour	1259:1279	A phylogenomic analysis of the core genome of the new strain and 5 closely related type strains of the genus Lysinibacillus revealed that this strain formed a distinct monophyletic clade with the nearest neighbour being Lysinibacillus boronitolerans.
30820713	9	27	theme	nearest	1263:1269	arg1	boronitolerans					1302:1315	Lysinibacillus boronitolerans	1287:1315	Lysinibacillus boronitolerans	1287:1315	A phylogenomic analysis of the core genome of the new strain and 5 closely related type strains of the genus Lysinibacillus revealed that this strain formed a distinct monophyletic clade with the nearest neighbour being Lysinibacillus boronitolerans.
30820713	11	28	theme	genus	1605:1609	arg1	Lysinibacillus					1611:1624	the genus Lysinibacillus	1601:1624	the genus Lysinibacillus	1601:1624	Based upon the consensus of phylogenetic and phenotypic analyses, we conclude that this strain represents a novel species within the genus Lysinibacillus, for which the name Lysinibacillus capsici sp.
30820713	0	29	theme	Lysinibacillus	0:13	arg1	sp					23:24	Lysinibacillus capsici sp	0:24	Lysinibacillus capsici sp.	0:25	Lysinibacillus capsici sp.
30820713	1	30	theme	plant	74:78	arg1	rhizosphere					50:60	the rhizosphere	46:60	the rhizosphere of a pepper plant	46:78	nov, isolated from the rhizosphere of a pepper plant.
30820713	3	31	theme	gene	350:353	arg1	sequences					355:363	the 16S rRNA gene sequences	337:363	the 16S rRNA gene sequences	337:363	Phylogenetic analysis of the 16S rRNA gene sequences indicated that this strain, PB300T, is closely related to Lysinibacillus macroides DMS 54T (99.6%) and Lysinibacillus xylanilyticus DSM 23493T (99.4%).
30820713	3	32	theme	macroides	438:446	arg1	54T					452:454	Lysinibacillus macroides DMS 54T	423:454	Lysinibacillus macroides DMS 54T (99.6%)	423:462	Phylogenetic analysis of the 16S rRNA gene sequences indicated that this strain, PB300T, is closely related to Lysinibacillus macroides DMS 54T (99.6%) and Lysinibacillus xylanilyticus DSM 23493T (99.4%).
30820713	3	32	theme	macroides	438:446	arg1	%					461:461	99.6%	457:461	99.6%	457:461	Phylogenetic analysis of the 16S rRNA gene sequences indicated that this strain, PB300T, is closely related to Lysinibacillus macroides DMS 54T (99.6%) and Lysinibacillus xylanilyticus DSM 23493T (99.4%).
30820713	7	33	theme	lysine-aspartic	921:935	arg1	acid					937:940	lysine-aspartic acid	921:940	lysine-aspartic acid	921:940	The cell wall peptidoglycan contains lysine-aspartic acid, as in its close relatives.
30820713	10	34	theme	%	1469:1469	arg1	threshold					1454:1462	the species threshold	1442:1462	the species threshold of 70%	1442:1469	DNA-DNA relatedness studies using in silico DNA-DNA hybridizations (DDH) showed relationships for the new strain were below the species threshold of 70%.
30820713	9	35	theme	phylogenomic	1069:1080	arg1	analysis					1082:1089	A phylogenomic analysis	1067:1089	A phylogenomic analysis of the core genome of the new strain and 5 closely related type strains of the genus Lysinibacillus	1067:1189	A phylogenomic analysis of the core genome of the new strain and 5 closely related type strains of the genus Lysinibacillus revealed that this strain formed a distinct monophyletic clade with the nearest neighbour being Lysinibacillus boronitolerans.
30820713	7	36	theme	cell	888:891	arg1	peptidoglycan					898:910	The cell wall peptidoglycan	884:910	The cell wall peptidoglycan	884:910	The cell wall peptidoglycan contains lysine-aspartic acid, as in its close relatives.
30820713	2	37	theme	bacterium	166:174	arg1	strain					83:88	A strain	81:88	A strain of a Gram-positive, strictly aerobic, motile, rod-shaped, endospore forming bacterium	81:174	A strain of a Gram-positive, strictly aerobic, motile, rod-shaped, endospore forming bacterium was originally isolated from rhizospheric soil of a pepper plant when screening and bioprospecting for plant beneficial microorganisms.
30820713	9	38	theme	core	1098:1101	arg1	genome					1103:1108	the core genome	1094:1108	the core genome of the new strain and 5 closely related type strains of the genus Lysinibacillus	1094:1189	A phylogenomic analysis of the core genome of the new strain and 5 closely related type strains of the genus Lysinibacillus revealed that this strain formed a distinct monophyletic clade with the nearest neighbour being Lysinibacillus boronitolerans.
30820713	4	39	theme	novel	553:557	arg1	strain					559:564	the novel strain	549:564	the novel strain	549:564	In phenotypic characterisation, the novel strain was found to grow between 15 and 40 °C and tolerate up to 10% (w/v) NaCl.
30820713	3	40	theme	Lysinibacillus	468:481	arg1	23493T					501:506	Lysinibacillus xylanilyticus DSM 23493T	468:506	Lysinibacillus xylanilyticus DSM 23493T (99.4%)	468:514	Phylogenetic analysis of the 16S rRNA gene sequences indicated that this strain, PB300T, is closely related to Lysinibacillus macroides DMS 54T (99.6%) and Lysinibacillus xylanilyticus DSM 23493T (99.4%).
30820713	3	40	theme	Lysinibacillus	468:481	arg1	%					513:513	99.4%	509:513	99.4%	509:513	Phylogenetic analysis of the 16S rRNA gene sequences indicated that this strain, PB300T, is closely related to Lysinibacillus macroides DMS 54T (99.6%) and Lysinibacillus xylanilyticus DSM 23493T (99.4%).
30820713	12	41	theme	= NRRL	1716:1721	arg1	B-65515T					1723:1730	= NRRL B-65515T	1716:1730	= NRRL B-65515T	1716:1730	nov. is proposed, with type strain PB300T (= NRRL B-65515T, = CCUG 72241T).
30820713	12	41	theme	= NRRL	1716:1721	arg1	72241T					1740:1745	 = CCUG 72241T	1732:1745	 = CCUG 72241T	1732:1745	nov. is proposed, with type strain PB300T (= NRRL B-65515T, = CCUG 72241T).
30820713	10	42	dep	in	1352:1353	arg1	silico					1355:1360	silico	1355:1360	silico	1355:1360	DNA-DNA relatedness studies using in silico DNA-DNA hybridizations (DDH) showed relationships for the new strain were below the species threshold of 70%.
30820713	11	43	theme	novel	1580:1584	arg1	species					1586:1592	a novel species	1578:1592	a novel species	1578:1592	Based upon the consensus of phylogenetic and phenotypic analyses, we conclude that this strain represents a novel species within the genus Lysinibacillus, for which the name Lysinibacillus capsici sp.
30820713	9	44	theme	new	1117:1119	arg1	strain					1121:1126	the new strain	1113:1126	the new strain	1113:1126	A phylogenomic analysis of the core genome of the new strain and 5 closely related type strains of the genus Lysinibacillus revealed that this strain formed a distinct monophyletic clade with the nearest neighbour being Lysinibacillus boronitolerans.
30820713	5	45	dep	10	704:705	arg1	to					701:702	to	701:702	to	701:702	Furthermore, the strain was found to grow in media with pH 5 to 10 (optimal growth at pH 7.0).
30820713	4	46	theme	phenotypic	520:529	arg1	characterisation					531:546	phenotypic characterisation	520:546	phenotypic characterisation	520:546	In phenotypic characterisation, the novel strain was found to grow between 15 and 40 °C and tolerate up to 10% (w/v) NaCl.
30820713	12	47	theme	type	1696:1699	arg1	PB300T					1708:1713	type strain PB300T	1696:1713	type strain PB300T (= NRRL B-65515T, = CCUG 72241T)	1696:1746	nov. is proposed, with type strain PB300T (= NRRL B-65515T, = CCUG 72241T).
30820713	6	48	theme	fatty	760:764	arg1	iso-C15 					792:799	iso-C15 	792:799	iso-C15 	792:799	The predominant cellular fatty acids were observed to be iso-C15 : 0 (56.6 %), anteiso-C15 : 0 (14.6%), C16 :1ω7C alcohol (9.3%) and C16 : 0 (7.1%).
30820713	6	48	theme	fatty	760:764	arg1	acids					766:770	The predominant cellular fatty acids	735:770	The predominant cellular fatty acids	735:770	The predominant cellular fatty acids were observed to be iso-C15 : 0 (56.6 %), anteiso-C15 : 0 (14.6%), C16 :1ω7C alcohol (9.3%) and C16 : 0 (7.1%).
30820713	8	49	theme	mol	1053:1055	arg1	%					1050:1050	37.5%	1046:1050	37.5% (mol content)	1046:1064	A draft genome was completed and the DNA G + C content was determined to be 37.5% (mol content).
30820713	8	49	theme	mol	1053:1055	arg1	content					1057:1063	mol content	1053:1063	mol content	1053:1063	A draft genome was completed and the DNA G + C content was determined to be 37.5% (mol content).
30820713	6	50	dep	observed	777:784	arg1	%					880:880	7.1%	877:880	7.1%	877:880	The predominant cellular fatty acids were observed to be iso-C15 : 0 (56.6 %), anteiso-C15 : 0 (14.6%), C16 :1ω7C alcohol (9.3%) and C16 : 0 (7.1%).
30820713	6	50	dep	observed	777:784	arg1	 0					827:828	 0	827:828	 0	827:828	The predominant cellular fatty acids were observed to be iso-C15 : 0 (56.6 %), anteiso-C15 : 0 (14.6%), C16 :1ω7C alcohol (9.3%) and C16 : 0 (7.1%).
30820713	6	50	dep	observed	777:784	arg1	alcohol					849:855	C16 :1ω7C alcohol	839:855	C16 :1ω7C alcohol (9.3%)	839:862	The predominant cellular fatty acids were observed to be iso-C15 : 0 (56.6 %), anteiso-C15 : 0 (14.6%), C16 :1ω7C alcohol (9.3%) and C16 : 0 (7.1%).
30820713	6	50	dep	observed	777:784	arg1	%					810:810	56.6 %	805:810	56.6 %	805:810	The predominant cellular fatty acids were observed to be iso-C15 : 0 (56.6 %), anteiso-C15 : 0 (14.6%), C16 :1ω7C alcohol (9.3%) and C16 : 0 (7.1%).
30820713	6	50	dep	observed	777:784	arg1	 0					873:874	 0	873:874	 0	873:874	The predominant cellular fatty acids were observed to be iso-C15 : 0 (56.6 %), anteiso-C15 : 0 (14.6%), C16 :1ω7C alcohol (9.3%) and C16 : 0 (7.1%).
30820713	6	50	dep	observed	777:784	arg1	%					835:835	14.6%	831:835	14.6%	831:835	The predominant cellular fatty acids were observed to be iso-C15 : 0 (56.6 %), anteiso-C15 : 0 (14.6%), C16 :1ω7C alcohol (9.3%) and C16 : 0 (7.1%).
30820713	6	50	dep	observed	777:784	arg1	%					861:861	9.3%	858:861	9.3%	858:861	The predominant cellular fatty acids were observed to be iso-C15 : 0 (56.6 %), anteiso-C15 : 0 (14.6%), C16 :1ω7C alcohol (9.3%) and C16 : 0 (7.1%).
30820713	6	50	dep	observed	777:784	arg1	 0					801:802	 0	801:802	 0	801:802	The predominant cellular fatty acids were observed to be iso-C15 : 0 (56.6 %), anteiso-C15 : 0 (14.6%), C16 :1ω7C alcohol (9.3%) and C16 : 0 (7.1%).
30820713	6	50	dep	observed	777:784	arg1	anteiso-C15 					814:825	anteiso-C15 	814:825	anteiso-C15 	814:825	The predominant cellular fatty acids were observed to be iso-C15 : 0 (56.6 %), anteiso-C15 : 0 (14.6%), C16 :1ω7C alcohol (9.3%) and C16 : 0 (7.1%).
30820713	3	51	theme	DSM	497:499	arg1	23493T					501:506	Lysinibacillus xylanilyticus DSM 23493T	468:506	Lysinibacillus xylanilyticus DSM 23493T (99.4%)	468:514	Phylogenetic analysis of the 16S rRNA gene sequences indicated that this strain, PB300T, is closely related to Lysinibacillus macroides DMS 54T (99.6%) and Lysinibacillus xylanilyticus DSM 23493T (99.4%).
30820713	3	51	theme	DSM	497:499	arg1	%					513:513	99.4%	509:513	99.4%	509:513	Phylogenetic analysis of the 16S rRNA gene sequences indicated that this strain, PB300T, is closely related to Lysinibacillus macroides DMS 54T (99.6%) and Lysinibacillus xylanilyticus DSM 23493T (99.4%).
30820713	6	52	theme	predominant	739:749	arg1	iso-C15 					792:799	iso-C15 	792:799	iso-C15 	792:799	The predominant cellular fatty acids were observed to be iso-C15 : 0 (56.6 %), anteiso-C15 : 0 (14.6%), C16 :1ω7C alcohol (9.3%) and C16 : 0 (7.1%).
30820713	6	52	theme	predominant	739:749	arg1	acids					766:770	The predominant cellular fatty acids	735:770	The predominant cellular fatty acids	735:770	The predominant cellular fatty acids were observed to be iso-C15 : 0 (56.6 %), anteiso-C15 : 0 (14.6%), C16 :1ω7C alcohol (9.3%) and C16 : 0 (7.1%).
30820713	7	53	contain	contains	912:919	arg2	acid					937:940	lysine-aspartic acid	921:940	lysine-aspartic acid	921:940	The cell wall peptidoglycan contains lysine-aspartic acid, as in its close relatives.
30820713	7	53	contain	contains	912:919	arg1	peptidoglycan					898:910	The cell wall peptidoglycan	884:910	The cell wall peptidoglycan	884:910	The cell wall peptidoglycan contains lysine-aspartic acid, as in its close relatives.
30820713	7	53	contain	contains	912:919	arg2	peptidoglycan					898:910	The cell wall peptidoglycan	884:910	The cell wall peptidoglycan	884:910	The cell wall peptidoglycan contains lysine-aspartic acid, as in its close relatives.
30820713	7	53	contain	contains	912:919	arg1	relatives					959:967	its close relatives	949:967	its close relatives	949:967	The cell wall peptidoglycan contains lysine-aspartic acid, as in its close relatives.
30820713	2	54	theme	beneficial	285:294	arg1	microorganisms					296:309	plant beneficial microorganisms	279:309	plant beneficial microorganisms	279:309	A strain of a Gram-positive, strictly aerobic, motile, rod-shaped, endospore forming bacterium was originally isolated from rhizospheric soil of a pepper plant when screening and bioprospecting for plant beneficial microorganisms.
30820713	9	55	theme	type	1150:1153	arg1	strains					1155:1161	5 closely related type strains	1132:1161	5 closely related type strains of the genus Lysinibacillus	1132:1189	A phylogenomic analysis of the core genome of the new strain and 5 closely related type strains of the genus Lysinibacillus revealed that this strain formed a distinct monophyletic clade with the nearest neighbour being Lysinibacillus boronitolerans.
30820713	10	56	theme	DNA-DNA	1318:1324	arg1	studies					1338:1344	DNA-DNA relatedness studies	1318:1344	DNA-DNA relatedness studies using in silico DNA-DNA hybridizations (DDH)	1318:1389	DNA-DNA relatedness studies using in silico DNA-DNA hybridizations (DDH) showed relationships for the new strain were below the species threshold of 70%.
30820713	11	57	theme	analyses	1528:1535	arg1	consensus					1487:1495	the consensus	1483:1495	the consensus of phylogenetic and phenotypic analyses	1483:1535	Based upon the consensus of phylogenetic and phenotypic analyses, we conclude that this strain represents a novel species within the genus Lysinibacillus, for which the name Lysinibacillus capsici sp.
30820713	10	58	theme	DNA-DNA	1362:1368	arg1	DDH					1386:1388	DDH	1386:1388	DDH	1386:1388	DNA-DNA relatedness studies using in silico DNA-DNA hybridizations (DDH) showed relationships for the new strain were below the species threshold of 70%.
30820713	10	58	theme	DNA-DNA	1362:1368	arg1	hybridizations					1370:1383	in silico DNA-DNA hybridizations	1352:1383	in silico DNA-DNA hybridizations (DDH)	1352:1389	DNA-DNA relatedness studies using in silico DNA-DNA hybridizations (DDH) showed relationships for the new strain were below the species threshold of 70%.
30820713	2	59	theme	rhizospheric	205:216	arg1	soil					218:221	rhizospheric soil	205:221	rhizospheric soil of a pepper plant when screening and bioprospecting for plant beneficial microorganisms	205:309	A strain of a Gram-positive, strictly aerobic, motile, rod-shaped, endospore forming bacterium was originally isolated from rhizospheric soil of a pepper plant when screening and bioprospecting for plant beneficial microorganisms.
30820713	5	60	theme	growth	716:721	arg1	pH					696:697	pH 5 to 10	696:705	pH 5 to 10 (optimal growth at pH 7.0)	696:732	Furthermore, the strain was found to grow in media with pH 5 to 10 (optimal growth at pH 7.0).
30820713	5	60	theme	growth	716:721	arg1	pH					726:727	optimal growth at pH 7.0	708:731	optimal growth at pH 7.0	708:731	Furthermore, the strain was found to grow in media with pH 5 to 10 (optimal growth at pH 7.0).
30820713	8	61	theme	G + C	1011:1015	arg1	%					1050:1050	37.5%	1046:1050	37.5% (mol content)	1046:1064	A draft genome was completed and the DNA G + C content was determined to be 37.5% (mol content).
30820713	8	61	theme	G + C	1011:1015	arg1	content					1017:1023	the DNA G + C content	1003:1023	the DNA G + C content	1003:1023	A draft genome was completed and the DNA G + C content was determined to be 37.5% (mol content).
30820713	10	62	theme	in	1352:1353	arg1	DDH					1386:1388	DDH	1386:1388	DDH	1386:1388	DNA-DNA relatedness studies using in silico DNA-DNA hybridizations (DDH) showed relationships for the new strain were below the species threshold of 70%.
30820713	10	62	theme	in	1352:1353	arg1	hybridizations					1370:1383	in silico DNA-DNA hybridizations	1352:1383	in silico DNA-DNA hybridizations (DDH)	1352:1389	DNA-DNA relatedness studies using in silico DNA-DNA hybridizations (DDH) showed relationships for the new strain were below the species threshold of 70%.
30820713	11	63	theme	capsici	1661:1667	arg1	sp					1669:1670	the name Lysinibacillus capsici sp	1637:1670	the name Lysinibacillus capsici sp	1637:1670	Based upon the consensus of phylogenetic and phenotypic analyses, we conclude that this strain represents a novel species within the genus Lysinibacillus, for which the name Lysinibacillus capsici sp.
30820713	2	64	theme	pepper	228:233	arg1	plant					235:239	a pepper plant	226:239	a pepper plant when screening and bioprospecting for plant beneficial microorganisms	226:309	A strain of a Gram-positive, strictly aerobic, motile, rod-shaped, endospore forming bacterium was originally isolated from rhizospheric soil of a pepper plant when screening and bioprospecting for plant beneficial microorganisms.
30820713	9	65	theme	distinct	1226:1233	arg1	clade					1248:1252	a distinct monophyletic clade	1224:1252	a distinct monophyletic clade	1224:1252	A phylogenomic analysis of the core genome of the new strain and 5 closely related type strains of the genus Lysinibacillus revealed that this strain formed a distinct monophyletic clade with the nearest neighbour being Lysinibacillus boronitolerans.
30820713	4	66	theme	10	624:625	arg1	%					626:626	%	626:626	%	626:626	In phenotypic characterisation, the novel strain was found to grow between 15 and 40 °C and tolerate up to 10% (w/v) NaCl.
30820713	4	67	dep	%	626:626	arg1	w/v					629:631	w/v	629:631	w/v	629:631	In phenotypic characterisation, the novel strain was found to grow between 15 and 40 °C and tolerate up to 10% (w/v) NaCl.
30820713	3	68	theme	rRNA	345:348	arg1	sequences					355:363	the 16S rRNA gene sequences	337:363	the 16S rRNA gene sequences	337:363	Phylogenetic analysis of the 16S rRNA gene sequences indicated that this strain, PB300T, is closely related to Lysinibacillus macroides DMS 54T (99.6%) and Lysinibacillus xylanilyticus DSM 23493T (99.4%).
30820713	0	69	theme	capsici	15:21	arg1	sp					23:24	Lysinibacillus capsici sp	0:24	Lysinibacillus capsici sp.	0:25	Lysinibacillus capsici sp.
30820713	1	70	theme	pepper	67:72	arg1	plant					74:78	a pepper plant	65:78	a pepper plant	65:78	nov, isolated from the rhizosphere of a pepper plant.
30820713	3	71	dep	Lysinibacillus	468:481	arg1	xylanilyticus					483:495	xylanilyticus	483:495	xylanilyticus	483:495	Phylogenetic analysis of the 16S rRNA gene sequences indicated that this strain, PB300T, is closely related to Lysinibacillus macroides DMS 54T (99.6%) and Lysinibacillus xylanilyticus DSM 23493T (99.4%).
30820713	3	72	theme	sequences	355:363	arg1	analysis					325:332	Phylogenetic analysis	312:332	Phylogenetic analysis of the 16S rRNA gene sequences	312:363	Phylogenetic analysis of the 16S rRNA gene sequences indicated that this strain, PB300T, is closely related to Lysinibacillus macroides DMS 54T (99.6%) and Lysinibacillus xylanilyticus DSM 23493T (99.4%).
30820713	12	73	dep	PB300T	1708:1713	arg1	B-65515T					1723:1730	= NRRL B-65515T	1716:1730	= NRRL B-65515T	1716:1730	nov. is proposed, with type strain PB300T (= NRRL B-65515T, = CCUG 72241T).
30820713	12	73	dep	PB300T	1708:1713	arg1	72241T					1740:1745	 = CCUG 72241T	1732:1745	 = CCUG 72241T	1732:1745	nov. is proposed, with type strain PB300T (= NRRL B-65515T, = CCUG 72241T).
30820713	3	74	theme	DMS	448:450	arg1	54T					452:454	Lysinibacillus macroides DMS 54T	423:454	Lysinibacillus macroides DMS 54T (99.6%)	423:462	Phylogenetic analysis of the 16S rRNA gene sequences indicated that this strain, PB300T, is closely related to Lysinibacillus macroides DMS 54T (99.6%) and Lysinibacillus xylanilyticus DSM 23493T (99.4%).
30820713	3	74	theme	DMS	448:450	arg1	%					461:461	99.6%	457:461	99.6%	457:461	Phylogenetic analysis of the 16S rRNA gene sequences indicated that this strain, PB300T, is closely related to Lysinibacillus macroides DMS 54T (99.6%) and Lysinibacillus xylanilyticus DSM 23493T (99.4%).
30820713	7	75	theme	wall	893:896	arg1	peptidoglycan					898:910	The cell wall peptidoglycan	884:910	The cell wall peptidoglycan	884:910	The cell wall peptidoglycan contains lysine-aspartic acid, as in its close relatives.
30820713	9	76	theme	genus	1170:1174	arg1	Lysinibacillus					1176:1189	the genus Lysinibacillus	1166:1189	the genus Lysinibacillus	1166:1189	A phylogenomic analysis of the core genome of the new strain and 5 closely related type strains of the genus Lysinibacillus revealed that this strain formed a distinct monophyletic clade with the nearest neighbour being Lysinibacillus boronitolerans.
30820713	6	77	theme	C16 :1ω7C	839:847	arg1	alcohol					849:855	C16 :1ω7C alcohol	839:855	C16 :1ω7C alcohol (9.3%)	839:862	The predominant cellular fatty acids were observed to be iso-C15 : 0 (56.6 %), anteiso-C15 : 0 (14.6%), C16 :1ω7C alcohol (9.3%) and C16 : 0 (7.1%).
30820713	6	77	theme	C16 :1ω7C	839:847	arg1	%					861:861	9.3%	858:861	9.3%	858:861	The predominant cellular fatty acids were observed to be iso-C15 : 0 (56.6 %), anteiso-C15 : 0 (14.6%), C16 :1ω7C alcohol (9.3%) and C16 : 0 (7.1%).
30820713	10	78	dep	showed	1391:1396	arg1	threshold					1454:1462	the species threshold	1442:1462	the species threshold of 70%	1442:1469	DNA-DNA relatedness studies using in silico DNA-DNA hybridizations (DDH) showed relationships for the new strain were below the species threshold of 70%.
30820713	3	79	theme	Lysinibacillus	423:436	arg1	54T					452:454	Lysinibacillus macroides DMS 54T	423:454	Lysinibacillus macroides DMS 54T (99.6%)	423:462	Phylogenetic analysis of the 16S rRNA gene sequences indicated that this strain, PB300T, is closely related to Lysinibacillus macroides DMS 54T (99.6%) and Lysinibacillus xylanilyticus DSM 23493T (99.4%).
30820713	3	79	theme	Lysinibacillus	423:436	arg1	%					461:461	99.6%	457:461	99.6%	457:461	Phylogenetic analysis of the 16S rRNA gene sequences indicated that this strain, PB300T, is closely related to Lysinibacillus macroides DMS 54T (99.6%) and Lysinibacillus xylanilyticus DSM 23493T (99.4%).
30820713	2	80	dep	Gram-positive	95:107	arg1	rod-shaped					136:145	rod-shaped	136:145	rod-shaped	136:145	A strain of a Gram-positive, strictly aerobic, motile, rod-shaped, endospore forming bacterium was originally isolated from rhizospheric soil of a pepper plant when screening and bioprospecting for plant beneficial microorganisms.
30820713	2	80	dep	Gram-positive	95:107	arg1	motile					128:133	motile	128:133	motile	128:133	A strain of a Gram-positive, strictly aerobic, motile, rod-shaped, endospore forming bacterium was originally isolated from rhizospheric soil of a pepper plant when screening and bioprospecting for plant beneficial microorganisms.
30820713	2	80	dep	Gram-positive	95:107	arg1	aerobic					119:125	aerobic	119:125	aerobic	119:125	A strain of a Gram-positive, strictly aerobic, motile, rod-shaped, endospore forming bacterium was originally isolated from rhizospheric soil of a pepper plant when screening and bioprospecting for plant beneficial microorganisms.
30820713	2	80	dep	Gram-positive	95:107	arg1	endospore					148:156	endospore	148:156	endospore	148:156	A strain of a Gram-positive, strictly aerobic, motile, rod-shaped, endospore forming bacterium was originally isolated from rhizospheric soil of a pepper plant when screening and bioprospecting for plant beneficial microorganisms.
30820713	4	81	located	found	570:574	arg2	strain					559:564	the novel strain	549:564	the novel strain	549:564	In phenotypic characterisation, the novel strain was found to grow between 15 and 40 °C and tolerate up to 10% (w/v) NaCl.
30820713	4	81	located	found	570:574	arg1	characterisation					531:546	phenotypic characterisation	520:546	phenotypic characterisation	520:546	In phenotypic characterisation, the novel strain was found to grow between 15 and 40 °C and tolerate up to 10% (w/v) NaCl.
30820713	2	82	theme	forming	158:164	arg1	bacterium					166:174	a Gram-positive, strictly aerobic, motile, rod-shaped, endospore forming bacterium	93:174	a Gram-positive, strictly aerobic, motile, rod-shaped, endospore forming bacterium	93:174	A strain of a Gram-positive, strictly aerobic, motile, rod-shaped, endospore forming bacterium was originally isolated from rhizospheric soil of a pepper plant when screening and bioprospecting for plant beneficial microorganisms.
30820713	10	83	theme	new	1420:1422	arg1	strain					1424:1429	the new strain	1416:1429	the new strain	1416:1429	DNA-DNA relatedness studies using in silico DNA-DNA hybridizations (DDH) showed relationships for the new strain were below the species threshold of 70%.
30820713	9	84	theme	genome	1103:1108	arg1	analysis					1082:1089	A phylogenomic analysis	1067:1089	A phylogenomic analysis of the core genome of the new strain and 5 closely related type strains of the genus Lysinibacillus	1067:1189	A phylogenomic analysis of the core genome of the new strain and 5 closely related type strains of the genus Lysinibacillus revealed that this strain formed a distinct monophyletic clade with the nearest neighbour being Lysinibacillus boronitolerans.
30820713	5	85	theme	at	723:724	arg1	pH					696:697	pH 5 to 10	696:705	pH 5 to 10 (optimal growth at pH 7.0)	696:732	Furthermore, the strain was found to grow in media with pH 5 to 10 (optimal growth at pH 7.0).
30820713	5	85	theme	at	723:724	arg1	pH					726:727	optimal growth at pH 7.0	708:731	optimal growth at pH 7.0	708:731	Furthermore, the strain was found to grow in media with pH 5 to 10 (optimal growth at pH 7.0).
30820713	2	86	theme	Gram-positive	95:107	arg1	bacterium					166:174	a Gram-positive, strictly aerobic, motile, rod-shaped, endospore forming bacterium	93:174	a Gram-positive, strictly aerobic, motile, rod-shaped, endospore forming bacterium	93:174	A strain of a Gram-positive, strictly aerobic, motile, rod-shaped, endospore forming bacterium was originally isolated from rhizospheric soil of a pepper plant when screening and bioprospecting for plant beneficial microorganisms.
30307388	2	0	theme	motile	102:107	arg1	bacterium					143:151	A Gram-stain-negative, aerobic, motile, endospore-forming and rod-shaped bacterium	70:151	A Gram-stain-negative, aerobic, motile, endospore-forming and rod-shaped bacterium	70:151	A Gram-stain-negative, aerobic, motile, endospore-forming and rod-shaped bacterium, designated RA17T, was isolated from Dafan, Hubei Province, China.
30307388	10	1	theme	diamino	1255:1261	arg1	acid					1309:1312	meso-diaminopimelic acid	1289:1312	meso-diaminopimelic acid	1289:1312	The diagnostic diamino acid of the cell wall was meso-diaminopimelic acid.
30307388	10	1	theme	diamino	1255:1261	arg1	acid					1263:1266	The diagnostic diamino acid	1240:1266	The diagnostic diamino acid of the cell wall	1240:1283	The diagnostic diamino acid of the cell wall was meso-diaminopimelic acid.
30307388	11	2	theme	such	1396:1399	arg1	differences					1383:1393	physiological and biochemical differences	1353:1393	physiological and biochemical differences	1353:1393	The low DNA-DNA hybridization values, physiological and biochemical differences, such as growth at 4 °C, acid production from inositol, lack of α-chymotrypsin activity, no casein hydrolysis, and negative for acid production from d-fructose, melibiose and sucrose, could distinguish strain RA17T from its closely related species.
30307388	2	3	attach	isolated	176:183	arg1	Hubei					197:201	Hubei	197:201	Hubei	197:201	A Gram-stain-negative, aerobic, motile, endospore-forming and rod-shaped bacterium, designated RA17T, was isolated from Dafan, Hubei Province, China.
30307388	2	3	attach	isolated	176:183	arg2	bacterium					143:151	A Gram-stain-negative, aerobic, motile, endospore-forming and rod-shaped bacterium	70:151	A Gram-stain-negative, aerobic, motile, endospore-forming and rod-shaped bacterium	70:151	A Gram-stain-negative, aerobic, motile, endospore-forming and rod-shaped bacterium, designated RA17T, was isolated from Dafan, Hubei Province, China.
30307388	2	3	attach	isolated	176:183	arg1	China					213:217	China	213:217	China	213:217	A Gram-stain-negative, aerobic, motile, endospore-forming and rod-shaped bacterium, designated RA17T, was isolated from Dafan, Hubei Province, China.
30307388	2	4	theme	rod-shaped	132:141	arg1	bacterium					143:151	A Gram-stain-negative, aerobic, motile, endospore-forming and rod-shaped bacterium	70:151	A Gram-stain-negative, aerobic, motile, endospore-forming and rod-shaped bacterium	70:151	A Gram-stain-negative, aerobic, motile, endospore-forming and rod-shaped bacterium, designated RA17T, was isolated from Dafan, Hubei Province, China.
30307388	7	5	theme	fatty	940:944	arg1	anteiso-C15 					957:968	anteiso-C15 	957:968	anteiso-C15 	957:968	The major cellular fatty acids were anteiso-C15 : 0, iso-C15 : 0 and iso-C16 : 0.
30307388	7	5	theme	fatty	940:944	arg1	acids					946:950	The major cellular fatty acids	921:950	The major cellular fatty acids	921:950	The major cellular fatty acids were anteiso-C15 : 0, iso-C15 : 0 and iso-C16 : 0.
30307388	10	6	theme	cell	1275:1278	arg1	wall					1280:1283	the cell wall	1271:1283	the cell wall	1271:1283	The diagnostic diamino acid of the cell wall was meso-diaminopimelic acid.
30307388	11	7	theme	no	1484:1485	arg1	hydrolysis					1494:1503	no casein hydrolysis	1484:1503	no casein hydrolysis	1484:1503	The low DNA-DNA hybridization values, physiological and biochemical differences, such as growth at 4 °C, acid production from inositol, lack of α-chymotrypsin activity, no casein hydrolysis, and negative for acid production from d-fructose, melibiose and sucrose, could distinguish strain RA17T from its closely related species.
30307388	11	7	theme	no	1484:1485	arg1	4 °C					1414:1417	4 °C	1414:1417	4 °C	1414:1417	The low DNA-DNA hybridization values, physiological and biochemical differences, such as growth at 4 °C, acid production from inositol, lack of α-chymotrypsin activity, no casein hydrolysis, and negative for acid production from d-fructose, melibiose and sucrose, could distinguish strain RA17T from its closely related species.
30307388	7	8	theme	major	925:929	arg1	anteiso-C15 					957:968	anteiso-C15 	957:968	anteiso-C15 	957:968	The major cellular fatty acids were anteiso-C15 : 0, iso-C15 : 0 and iso-C16 : 0.
30307388	7	8	theme	major	925:929	arg1	acids					946:950	The major cellular fatty acids	921:950	The major cellular fatty acids	921:950	The major cellular fatty acids were anteiso-C15 : 0, iso-C15 : 0 and iso-C16 : 0.
30307388	8	9	theme	polar	1007:1011	arg1	diphosphatidylglycerol					1025:1046	diphosphatidylglycerol	1025:1046	diphosphatidylglycerol	1025:1046	The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphoglycolipid, four aminophospholipids, an unidentified phospholipid and an unidentified polar lipid.
30307388	8	9	theme	polar	1007:1011	arg1	lipids					1013:1018	The polar lipids	1003:1018	The polar lipids	1003:1018	The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphoglycolipid, four aminophospholipids, an unidentified phospholipid and an unidentified polar lipid.
30307388	6	10	theme	genome	825:830	arg1	size					832:835	Its genome size	821:835	Its genome size	821:835	Its genome size was 6.17 Mb, comprising 5677 predicted genes with a DNA G+C content of 52.82 mol %.
30307388	3	11	theme	sequence	243:250	arg1	comparisons					263:273	16S rRNA gene sequence similarity comparisons	229:273	16S rRNA gene sequence similarity comparisons	229:273	Based on 16S rRNA gene sequence similarity comparisons, strain RA17T was most closely related to Paenibacillus taihuensis THMBG22T (97.4 %), Paenibacillus rhizoryzae ACCC 1ZS3-5T (97.4 %) and Paenibacillus sacheonensis DSM SY01T (96.5 %).
30307388	5	12	theme	type	716:719	arg1	strains					721:727	the two type strains	708:727	the two type strains	708:727	The DNA-DNA hybridization values between strain RA17T and the two type strains, P. taihuensis THMBG22T and P. rhizoryzae ACCC 1ZS3-5T, were 36.8 and 22.9 %, respectively.
30307388	5	12	theme	type	716:719	arg1	1ZS3-5T					776:782	P. rhizoryzae ACCC 1ZS3-5T	757:782	P. rhizoryzae ACCC 1ZS3-5T	757:782	The DNA-DNA hybridization values between strain RA17T and the two type strains, P. taihuensis THMBG22T and P. rhizoryzae ACCC 1ZS3-5T, were 36.8 and 22.9 %, respectively.
30307388	5	12	theme	type	716:719	arg1	THMBG22T					744:751	P. taihuensis THMBG22T	730:751	P. taihuensis THMBG22T	730:751	The DNA-DNA hybridization values between strain RA17T and the two type strains, P. taihuensis THMBG22T and P. rhizoryzae ACCC 1ZS3-5T, were 36.8 and 22.9 %, respectively.
30307388	3	13	theme	rRNA	233:236	arg1	comparisons					263:273	16S rRNA gene sequence similarity comparisons	229:273	16S rRNA gene sequence similarity comparisons	229:273	Based on 16S rRNA gene sequence similarity comparisons, strain RA17T was most closely related to Paenibacillus taihuensis THMBG22T (97.4 %), Paenibacillus rhizoryzae ACCC 1ZS3-5T (97.4 %) and Paenibacillus sacheonensis DSM SY01T (96.5 %).
30307388	2	14	theme	aerobic	93:99	arg1	bacterium					143:151	A Gram-stain-negative, aerobic, motile, endospore-forming and rod-shaped bacterium	70:151	A Gram-stain-negative, aerobic, motile, endospore-forming and rod-shaped bacterium	70:151	A Gram-stain-negative, aerobic, motile, endospore-forming and rod-shaped bacterium, designated RA17T, was isolated from Dafan, Hubei Province, China.
30307388	3	15	theme	strain	276:281	arg1	RA17T					283:287	strain RA17T	276:287	strain RA17T	276:287	Based on 16S rRNA gene sequence similarity comparisons, strain RA17T was most closely related to Paenibacillus taihuensis THMBG22T (97.4 %), Paenibacillus rhizoryzae ACCC 1ZS3-5T (97.4 %) and Paenibacillus sacheonensis DSM SY01T (96.5 %).
30307388	2	16	theme	Gram-stain-negative	72:90	arg1	bacterium					143:151	A Gram-stain-negative, aerobic, motile, endospore-forming and rod-shaped bacterium	70:151	A Gram-stain-negative, aerobic, motile, endospore-forming and rod-shaped bacterium	70:151	A Gram-stain-negative, aerobic, motile, endospore-forming and rod-shaped bacterium, designated RA17T, was isolated from Dafan, Hubei Province, China.
30307388	13	17	theme	=KCTC	1804:1808	arg1	2017053T					1826:1833	=KCTC 33894T=CCTCC AB 2017053T	1804:1833	=KCTC 33894T=CCTCC AB 2017053T	1804:1833	nov. is proposed, with RA17T (=KCTC 33894T=CCTCC AB 2017053T) as the type strain.
30307388	13	17	theme	=KCTC	1804:1808	arg1	RA17T					1797:1801	RA17T	1797:1801	RA17T (=KCTC 33894T=CCTCC AB 2017053T) as the type strain	1797:1853	nov. is proposed, with RA17T (=KCTC 33894T=CCTCC AB 2017053T) as the type strain.
30307388	11	18	theme	physiological	1353:1365	arg1	differences					1383:1393	physiological and biochemical differences	1353:1393	physiological and biochemical differences	1353:1393	The low DNA-DNA hybridization values, physiological and biochemical differences, such as growth at 4 °C, acid production from inositol, lack of α-chymotrypsin activity, no casein hydrolysis, and negative for acid production from d-fructose, melibiose and sucrose, could distinguish strain RA17T from its closely related species.
30307388	11	19	theme	strain	1597:1602	arg1	RA17T					1604:1608	strain RA17T	1597:1608	strain RA17T	1597:1608	The low DNA-DNA hybridization values, physiological and biochemical differences, such as growth at 4 °C, acid production from inositol, lack of α-chymotrypsin activity, no casein hydrolysis, and negative for acid production from d-fructose, melibiose and sucrose, could distinguish strain RA17T from its closely related species.
30307388	4	20	dep	P.	541:542	arg1	rhizoryzae					544:553	rhizoryzae	544:553	rhizoryzae	544:553	Analysis of the rpoB gene also indicated that RA17T had the highest similarity to P. rhizoryzae ACCC 1ZS3-5T (92.3 %), P. taihuensis THMBG22T (88.4 %) and P. sacheonensis DSM SY01T (85.5 %).
30307388	11	21	from	melibiose	1556:1564	arg1	production					1528:1537	acid production	1523:1537	acid production from d-fructose, melibiose and sucrose	1523:1576	The low DNA-DNA hybridization values, physiological and biochemical differences, such as growth at 4 °C, acid production from inositol, lack of α-chymotrypsin activity, no casein hydrolysis, and negative for acid production from d-fructose, melibiose and sucrose, could distinguish strain RA17T from its closely related species.
30307388	11	22	from	sucrose	1570:1576	arg1	production					1528:1537	acid production	1523:1537	acid production from d-fructose, melibiose and sucrose	1523:1576	The low DNA-DNA hybridization values, physiological and biochemical differences, such as growth at 4 °C, acid production from inositol, lack of α-chymotrypsin activity, no casein hydrolysis, and negative for acid production from d-fructose, melibiose and sucrose, could distinguish strain RA17T from its closely related species.
30307388	8	23	theme	polar	1190:1194	arg1	lipid					1196:1200	an unidentified polar lipid	1174:1200	an unidentified polar lipid	1174:1200	The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphoglycolipid, four aminophospholipids, an unidentified phospholipid and an unidentified polar lipid.
30307388	3	24	theme	taihuensis	331:340	arg1	%					357:357	97.4 %	352:357	97.4 %	352:357	Based on 16S rRNA gene sequence similarity comparisons, strain RA17T was most closely related to Paenibacillus taihuensis THMBG22T (97.4 %), Paenibacillus rhizoryzae ACCC 1ZS3-5T (97.4 %) and Paenibacillus sacheonensis DSM SY01T (96.5 %).
30307388	3	24	theme	taihuensis	331:340	arg1	THMBG22T					342:349	Paenibacillus taihuensis THMBG22T	317:349	Paenibacillus taihuensis THMBG22T (97.4 %)	317:358	Based on 16S rRNA gene sequence similarity comparisons, strain RA17T was most closely related to Paenibacillus taihuensis THMBG22T (97.4 %), Paenibacillus rhizoryzae ACCC 1ZS3-5T (97.4 %) and Paenibacillus sacheonensis DSM SY01T (96.5 %).
30307388	13	25	theme	33894T=CCTCC	1810:1821	arg1	2017053T					1826:1833	=KCTC 33894T=CCTCC AB 2017053T	1804:1833	=KCTC 33894T=CCTCC AB 2017053T	1804:1833	nov. is proposed, with RA17T (=KCTC 33894T=CCTCC AB 2017053T) as the type strain.
30307388	13	25	theme	33894T=CCTCC	1810:1821	arg1	RA17T					1797:1801	RA17T	1797:1801	RA17T (=KCTC 33894T=CCTCC AB 2017053T) as the type strain	1797:1853	nov. is proposed, with RA17T (=KCTC 33894T=CCTCC AB 2017053T) as the type strain.
30307388	11	26	theme	biochemical	1371:1381	arg1	differences					1383:1393	physiological and biochemical differences	1353:1393	physiological and biochemical differences	1353:1393	The low DNA-DNA hybridization values, physiological and biochemical differences, such as growth at 4 °C, acid production from inositol, lack of α-chymotrypsin activity, no casein hydrolysis, and negative for acid production from d-fructose, melibiose and sucrose, could distinguish strain RA17T from its closely related species.
30307388	1	27	theme	area	59:62	arg1	soil					64:67	mountain area soil	50:67	mountain area soil	50:67	nov., isolated from mountain area soil.
30307388	4	28	theme	P.	614:615	arg1	SY01T					634:638	P. sacheonensis DSM SY01T	614:638	P. sacheonensis DSM SY01T (85.5 %)	614:647	Analysis of the rpoB gene also indicated that RA17T had the highest similarity to P. rhizoryzae ACCC 1ZS3-5T (92.3 %), P. taihuensis THMBG22T (88.4 %) and P. sacheonensis DSM SY01T (85.5 %).
30307388	4	28	theme	P.	614:615	arg1	%					646:646	85.5 %	641:646	85.5 %	641:646	Analysis of the rpoB gene also indicated that RA17T had the highest similarity to P. rhizoryzae ACCC 1ZS3-5T (92.3 %), P. taihuensis THMBG22T (88.4 %) and P. sacheonensis DSM SY01T (85.5 %).
30307388	11	29	from	d-fructose	1544:1553	arg1	production					1528:1537	acid production	1523:1537	acid production from d-fructose, melibiose and sucrose	1523:1576	The low DNA-DNA hybridization values, physiological and biochemical differences, such as growth at 4 °C, acid production from inositol, lack of α-chymotrypsin activity, no casein hydrolysis, and negative for acid production from d-fructose, melibiose and sucrose, could distinguish strain RA17T from its closely related species.
30307388	6	30	theme	%	918:918	arg1	G+C content					893:903	a DNA G+C content	887:903	a DNA G+C content of 52.82 mol %	887:918	Its genome size was 6.17 Mb, comprising 5677 predicted genes with a DNA G+C content of 52.82 mol %.
30307388	0	31	theme	Paenibacillus	0:12	arg1	montanisoli					14:24	Paenibacillus montanisoli	0:24	Paenibacillus montanisoli	0:24	Paenibacillus montanisoli sp.
30307388	12	32	theme	name	1740:1743	arg1	sp					1770:1771	the name Paenibacillusmontanisoli sp	1736:1771	the name Paenibacillusmontanisoli sp	1736:1771	Consequently, strain RA17T represents a novel species of the genus Paenibacillus, for which the name Paenibacillusmontanisoli sp.
30307388	4	33	dep	P.	614:615	arg1	sacheonensis					617:628	sacheonensis	617:628	sacheonensis	617:628	Analysis of the rpoB gene also indicated that RA17T had the highest similarity to P. rhizoryzae ACCC 1ZS3-5T (92.3 %), P. taihuensis THMBG22T (88.4 %) and P. sacheonensis DSM SY01T (85.5 %).
30307388	5	34	dep	P.	757:758	arg1	rhizoryzae					760:769	rhizoryzae	760:769	rhizoryzae	760:769	The DNA-DNA hybridization values between strain RA17T and the two type strains, P. taihuensis THMBG22T and P. rhizoryzae ACCC 1ZS3-5T, were 36.8 and 22.9 %, respectively.
30307388	11	35	theme	DNA-DNA	1323:1329	arg1	values					1345:1350	The low DNA-DNA hybridization values	1315:1350	The low DNA-DNA hybridization values	1315:1350	The low DNA-DNA hybridization values, physiological and biochemical differences, such as growth at 4 °C, acid production from inositol, lack of α-chymotrypsin activity, no casein hydrolysis, and negative for acid production from d-fructose, melibiose and sucrose, could distinguish strain RA17T from its closely related species.
30307388	3	36	theme	Paenibacillus	412:424	arg1	SY01T					443:447	Paenibacillus sacheonensis DSM SY01T	412:447	Paenibacillus sacheonensis DSM SY01T (96.5 %)	412:456	Based on 16S rRNA gene sequence similarity comparisons, strain RA17T was most closely related to Paenibacillus taihuensis THMBG22T (97.4 %), Paenibacillus rhizoryzae ACCC 1ZS3-5T (97.4 %) and Paenibacillus sacheonensis DSM SY01T (96.5 %).
30307388	3	36	theme	Paenibacillus	412:424	arg1	%					455:455	96.5 %	450:455	96.5 %	450:455	Based on 16S rRNA gene sequence similarity comparisons, strain RA17T was most closely related to Paenibacillus taihuensis THMBG22T (97.4 %), Paenibacillus rhizoryzae ACCC 1ZS3-5T (97.4 %) and Paenibacillus sacheonensis DSM SY01T (96.5 %).
30307388	12	37	theme	genus	1705:1709	arg1	species					1690:1696	a novel species	1682:1696	a novel species	1682:1696	Consequently, strain RA17T represents a novel species of the genus Paenibacillus, for which the name Paenibacillusmontanisoli sp.
30307388	3	38	theme	rhizoryzae	375:384	arg1	1ZS3-5T					391:397	Paenibacillus rhizoryzae ACCC 1ZS3-5T	361:397	Paenibacillus rhizoryzae ACCC 1ZS3-5T (97.4 %)	361:406	Based on 16S rRNA gene sequence similarity comparisons, strain RA17T was most closely related to Paenibacillus taihuensis THMBG22T (97.4 %), Paenibacillus rhizoryzae ACCC 1ZS3-5T (97.4 %) and Paenibacillus sacheonensis DSM SY01T (96.5 %).
30307388	3	38	theme	rhizoryzae	375:384	arg1	%					405:405	97.4 %	400:405	97.4 %	400:405	Based on 16S rRNA gene sequence similarity comparisons, strain RA17T was most closely related to Paenibacillus taihuensis THMBG22T (97.4 %), Paenibacillus rhizoryzae ACCC 1ZS3-5T (97.4 %) and Paenibacillus sacheonensis DSM SY01T (96.5 %).
30307388	5	39	theme	hybridization	662:674	arg1	values					676:681	The DNA-DNA hybridization values	650:681	The DNA-DNA hybridization values between strain RA17T and the two type strains, P. taihuensis THMBG22T and P. rhizoryzae ACCC 1ZS3-5T,	650:783	The DNA-DNA hybridization values between strain RA17T and the two type strains, P. taihuensis THMBG22T and P. rhizoryzae ACCC 1ZS3-5T, were 36.8 and 22.9 %, respectively.
30307388	5	39	theme	hybridization	662:674	arg1	%					804:804	36.8 and 22.9 %	790:804	36.8 and 22.9 %	790:804	The DNA-DNA hybridization values between strain RA17T and the two type strains, P. taihuensis THMBG22T and P. rhizoryzae ACCC 1ZS3-5T, were 36.8 and 22.9 %, respectively.
30307388	5	40	dep	P.	730:731	arg1	taihuensis					733:742	taihuensis	733:742	taihuensis	733:742	The DNA-DNA hybridization values between strain RA17T and the two type strains, P. taihuensis THMBG22T and P. rhizoryzae ACCC 1ZS3-5T, were 36.8 and 22.9 %, respectively.
30307388	12	41	theme	novel	1684:1688	arg1	species					1690:1696	a novel species	1682:1696	a novel species	1682:1696	Consequently, strain RA17T represents a novel species of the genus Paenibacillus, for which the name Paenibacillusmontanisoli sp.
30307388	4	42	theme	rpoB	475:478	arg1	gene					480:483	the rpoB gene	471:483	the rpoB gene	471:483	Analysis of the rpoB gene also indicated that RA17T had the highest similarity to P. rhizoryzae ACCC 1ZS3-5T (92.3 %), P. taihuensis THMBG22T (88.4 %) and P. sacheonensis DSM SY01T (85.5 %).
30307388	11	43	theme	acid	1420:1423	arg1	4 °C					1414:1417	4 °C	1414:1417	4 °C	1414:1417	The low DNA-DNA hybridization values, physiological and biochemical differences, such as growth at 4 °C, acid production from inositol, lack of α-chymotrypsin activity, no casein hydrolysis, and negative for acid production from d-fructose, melibiose and sucrose, could distinguish strain RA17T from its closely related species.
30307388	11	43	theme	acid	1420:1423	arg1	production					1425:1434	acid production	1420:1434	acid production from inositol	1420:1448	The low DNA-DNA hybridization values, physiological and biochemical differences, such as growth at 4 °C, acid production from inositol, lack of α-chymotrypsin activity, no casein hydrolysis, and negative for acid production from d-fructose, melibiose and sucrose, could distinguish strain RA17T from its closely related species.
30307388	3	44	theme	DSM	439:441	arg1	SY01T					443:447	Paenibacillus sacheonensis DSM SY01T	412:447	Paenibacillus sacheonensis DSM SY01T (96.5 %)	412:456	Based on 16S rRNA gene sequence similarity comparisons, strain RA17T was most closely related to Paenibacillus taihuensis THMBG22T (97.4 %), Paenibacillus rhizoryzae ACCC 1ZS3-5T (97.4 %) and Paenibacillus sacheonensis DSM SY01T (96.5 %).
30307388	3	44	theme	DSM	439:441	arg1	%					455:455	96.5 %	450:455	96.5 %	450:455	Based on 16S rRNA gene sequence similarity comparisons, strain RA17T was most closely related to Paenibacillus taihuensis THMBG22T (97.4 %), Paenibacillus rhizoryzae ACCC 1ZS3-5T (97.4 %) and Paenibacillus sacheonensis DSM SY01T (96.5 %).
30307388	0	45	dep	sp	26:27	arg1	montanisoli					14:24	Paenibacillus montanisoli	0:24	Paenibacillus montanisoli	0:24	Paenibacillus montanisoli sp.
30307388	11	46	theme	α-chymotrypsin	1459:1472	arg1	activity					1474:1481	α-chymotrypsin activity	1459:1481	α-chymotrypsin activity	1459:1481	The low DNA-DNA hybridization values, physiological and biochemical differences, such as growth at 4 °C, acid production from inositol, lack of α-chymotrypsin activity, no casein hydrolysis, and negative for acid production from d-fructose, melibiose and sucrose, could distinguish strain RA17T from its closely related species.
30307388	11	47	theme	casein	1487:1492	arg1	hydrolysis					1494:1503	no casein hydrolysis	1484:1503	no casein hydrolysis	1484:1503	The low DNA-DNA hybridization values, physiological and biochemical differences, such as growth at 4 °C, acid production from inositol, lack of α-chymotrypsin activity, no casein hydrolysis, and negative for acid production from d-fructose, melibiose and sucrose, could distinguish strain RA17T from its closely related species.
30307388	11	47	theme	casein	1487:1492	arg1	4 °C					1414:1417	4 °C	1414:1417	4 °C	1414:1417	The low DNA-DNA hybridization values, physiological and biochemical differences, such as growth at 4 °C, acid production from inositol, lack of α-chymotrypsin activity, no casein hydrolysis, and negative for acid production from d-fructose, melibiose and sucrose, could distinguish strain RA17T from its closely related species.
30307388	10	48	theme	diagnostic	1244:1253	arg1	acid					1309:1312	meso-diaminopimelic acid	1289:1312	meso-diaminopimelic acid	1289:1312	The diagnostic diamino acid of the cell wall was meso-diaminopimelic acid.
30307388	10	48	theme	diagnostic	1244:1253	arg1	acid					1263:1266	The diagnostic diamino acid	1240:1266	The diagnostic diamino acid of the cell wall	1240:1283	The diagnostic diamino acid of the cell wall was meso-diaminopimelic acid.
30307388	4	49	theme	highest	519:525	arg1	similarity					527:536	the highest similarity	515:536	the highest similarity to P. rhizoryzae ACCC 1ZS3-5T (92.3 %), P. taihuensis THMBG22T (88.4 %) and P. sacheonensis DSM SY01T (85.5 %)	515:647	Analysis of the rpoB gene also indicated that RA17T had the highest similarity to P. rhizoryzae ACCC 1ZS3-5T (92.3 %), P. taihuensis THMBG22T (88.4 %) and P. sacheonensis DSM SY01T (85.5 %).
30307388	11	50	theme	acid	1523:1526	arg1	production					1528:1537	acid production	1523:1537	acid production from d-fructose, melibiose and sucrose	1523:1576	The low DNA-DNA hybridization values, physiological and biochemical differences, such as growth at 4 °C, acid production from inositol, lack of α-chymotrypsin activity, no casein hydrolysis, and negative for acid production from d-fructose, melibiose and sucrose, could distinguish strain RA17T from its closely related species.
30307388	7	51	theme	cellular	931:938	arg1	anteiso-C15 					957:968	anteiso-C15 	957:968	anteiso-C15 	957:968	The major cellular fatty acids were anteiso-C15 : 0, iso-C15 : 0 and iso-C16 : 0.
30307388	7	51	theme	cellular	931:938	arg1	acids					946:950	The major cellular fatty acids	921:950	The major cellular fatty acids	921:950	The major cellular fatty acids were anteiso-C15 : 0, iso-C15 : 0 and iso-C16 : 0.
30307388	13	52	theme	AB	1823:1824	arg1	2017053T					1826:1833	=KCTC 33894T=CCTCC AB 2017053T	1804:1833	=KCTC 33894T=CCTCC AB 2017053T	1804:1833	nov. is proposed, with RA17T (=KCTC 33894T=CCTCC AB 2017053T) as the type strain.
30307388	13	52	theme	AB	1823:1824	arg1	RA17T					1797:1801	RA17T	1797:1801	RA17T (=KCTC 33894T=CCTCC AB 2017053T) as the type strain	1797:1853	nov. is proposed, with RA17T (=KCTC 33894T=CCTCC AB 2017053T) as the type strain.
30307388	5	53	theme	P.	730:731	arg1	strains					721:727	the two type strains	708:727	the two type strains	708:727	The DNA-DNA hybridization values between strain RA17T and the two type strains, P. taihuensis THMBG22T and P. rhizoryzae ACCC 1ZS3-5T, were 36.8 and 22.9 %, respectively.
30307388	5	53	theme	P.	730:731	arg1	THMBG22T					744:751	P. taihuensis THMBG22T	730:751	P. taihuensis THMBG22T	730:751	The DNA-DNA hybridization values between strain RA17T and the two type strains, P. taihuensis THMBG22T and P. rhizoryzae ACCC 1ZS3-5T, were 36.8 and 22.9 %, respectively.
30307388	3	54	theme	16S	229:231	arg1	comparisons					263:273	16S rRNA gene sequence similarity comparisons	229:273	16S rRNA gene sequence similarity comparisons	229:273	Based on 16S rRNA gene sequence similarity comparisons, strain RA17T was most closely related to Paenibacillus taihuensis THMBG22T (97.4 %), Paenibacillus rhizoryzae ACCC 1ZS3-5T (97.4 %) and Paenibacillus sacheonensis DSM SY01T (96.5 %).
30307388	11	55	theme	negative	1510:1517	arg1	differences					1383:1393	physiological and biochemical differences	1353:1393	physiological and biochemical differences	1353:1393	The low DNA-DNA hybridization values, physiological and biochemical differences, such as growth at 4 °C, acid production from inositol, lack of α-chymotrypsin activity, no casein hydrolysis, and negative for acid production from d-fructose, melibiose and sucrose, could distinguish strain RA17T from its closely related species.
30307388	7	56	dep	anteiso-C15 	957:968	arg1	 0					999:1000	 0	999:1000	 0	999:1000	The major cellular fatty acids were anteiso-C15 : 0, iso-C15 : 0 and iso-C16 : 0.
30307388	7	56	dep	anteiso-C15 	957:968	arg1	iso-C16 					990:997	iso-C16 	990:997	iso-C16 	990:997	The major cellular fatty acids were anteiso-C15 : 0, iso-C15 : 0 and iso-C16 : 0.
30307388	7	56	dep	anteiso-C15 	957:968	arg1	 0					970:971	 0	970:971	 0	970:971	The major cellular fatty acids were anteiso-C15 : 0, iso-C15 : 0 and iso-C16 : 0.
30307388	7	56	dep	anteiso-C15 	957:968	arg1	 0					983:984	 0	983:984	 0	983:984	The major cellular fatty acids were anteiso-C15 : 0, iso-C15 : 0 and iso-C16 : 0.
30307388	3	57	theme	similarity	252:261	arg1	comparisons					263:273	16S rRNA gene sequence similarity comparisons	229:273	16S rRNA gene sequence similarity comparisons	229:273	Based on 16S rRNA gene sequence similarity comparisons, strain RA17T was most closely related to Paenibacillus taihuensis THMBG22T (97.4 %), Paenibacillus rhizoryzae ACCC 1ZS3-5T (97.4 %) and Paenibacillus sacheonensis DSM SY01T (96.5 %).
30307388	3	58	theme	gene	238:241	arg1	comparisons					263:273	16S rRNA gene sequence similarity comparisons	229:273	16S rRNA gene sequence similarity comparisons	229:273	Based on 16S rRNA gene sequence similarity comparisons, strain RA17T was most closely related to Paenibacillus taihuensis THMBG22T (97.4 %), Paenibacillus rhizoryzae ACCC 1ZS3-5T (97.4 %) and Paenibacillus sacheonensis DSM SY01T (96.5 %).
30307388	4	59	contain	had	511:513	arg2	similarity					527:536	the highest similarity	515:536	the highest similarity to P. rhizoryzae ACCC 1ZS3-5T (92.3 %), P. taihuensis THMBG22T (88.4 %) and P. sacheonensis DSM SY01T (85.5 %)	515:647	Analysis of the rpoB gene also indicated that RA17T had the highest similarity to P. rhizoryzae ACCC 1ZS3-5T (92.3 %), P. taihuensis THMBG22T (88.4 %) and P. sacheonensis DSM SY01T (85.5 %).
30307388	4	59	contain	had	511:513	arg1	RA17T					505:509	RA17T	505:509	RA17T	505:509	Analysis of the rpoB gene also indicated that RA17T had the highest similarity to P. rhizoryzae ACCC 1ZS3-5T (92.3 %), P. taihuensis THMBG22T (88.4 %) and P. sacheonensis DSM SY01T (85.5 %).
30307388	11	60	from	4 °C	1414:1417	arg1	growth					1404:1409	growth	1404:1409	growth at 4 °C, acid production from inositol, lack of α-chymotrypsin activity, no casein hydrolysis,	1404:1504	The low DNA-DNA hybridization values, physiological and biochemical differences, such as growth at 4 °C, acid production from inositol, lack of α-chymotrypsin activity, no casein hydrolysis, and negative for acid production from d-fructose, melibiose and sucrose, could distinguish strain RA17T from its closely related species.
30307388	8	61	theme	unidentified	1144:1155	arg1	phospholipid					1157:1168	an unidentified phospholipid	1141:1168	an unidentified phospholipid	1141:1168	The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphoglycolipid, four aminophospholipids, an unidentified phospholipid and an unidentified polar lipid.
30307388	11	62	theme	hybridization	1331:1343	arg1	values					1345:1350	The low DNA-DNA hybridization values	1315:1350	The low DNA-DNA hybridization values	1315:1350	The low DNA-DNA hybridization values, physiological and biochemical differences, such as growth at 4 °C, acid production from inositol, lack of α-chymotrypsin activity, no casein hydrolysis, and negative for acid production from d-fructose, melibiose and sucrose, could distinguish strain RA17T from its closely related species.
30307388	5	63	theme	P.	757:758	arg1	strains					721:727	the two type strains	708:727	the two type strains	708:727	The DNA-DNA hybridization values between strain RA17T and the two type strains, P. taihuensis THMBG22T and P. rhizoryzae ACCC 1ZS3-5T, were 36.8 and 22.9 %, respectively.
30307388	5	63	theme	P.	757:758	arg1	1ZS3-5T					776:782	P. rhizoryzae ACCC 1ZS3-5T	757:782	P. rhizoryzae ACCC 1ZS3-5T	757:782	The DNA-DNA hybridization values between strain RA17T and the two type strains, P. taihuensis THMBG22T and P. rhizoryzae ACCC 1ZS3-5T, were 36.8 and 22.9 %, respectively.
30307388	6	64	theme	predicted	866:874	arg1	genes					876:880	5677 predicted genes	861:880	5677 predicted genes	861:880	Its genome size was 6.17 Mb, comprising 5677 predicted genes with a DNA G+C content of 52.82 mol %.
30307388	5	65	theme	ACCC	771:774	arg1	strains					721:727	the two type strains	708:727	the two type strains	708:727	The DNA-DNA hybridization values between strain RA17T and the two type strains, P. taihuensis THMBG22T and P. rhizoryzae ACCC 1ZS3-5T, were 36.8 and 22.9 %, respectively.
30307388	5	65	theme	ACCC	771:774	arg1	1ZS3-5T					776:782	P. rhizoryzae ACCC 1ZS3-5T	757:782	P. rhizoryzae ACCC 1ZS3-5T	757:782	The DNA-DNA hybridization values between strain RA17T and the two type strains, P. taihuensis THMBG22T and P. rhizoryzae ACCC 1ZS3-5T, were 36.8 and 22.9 %, respectively.
30307388	10	66	theme	meso-diaminopimelic	1289:1307	arg1	acid					1309:1312	meso-diaminopimelic acid	1289:1312	meso-diaminopimelic acid	1289:1312	The diagnostic diamino acid of the cell wall was meso-diaminopimelic acid.
30307388	10	66	theme	meso-diaminopimelic	1289:1307	arg1	acid					1263:1266	The diagnostic diamino acid	1240:1266	The diagnostic diamino acid of the cell wall	1240:1283	The diagnostic diamino acid of the cell wall was meso-diaminopimelic acid.
30307388	8	67	theme	unidentified	1177:1188	arg1	lipid					1196:1200	an unidentified polar lipid	1174:1200	an unidentified polar lipid	1174:1200	The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphoglycolipid, four aminophospholipids, an unidentified phospholipid and an unidentified polar lipid.
30307388	11	68	theme	related	1627:1633	arg1	species					1635:1641	its closely related species	1615:1641	its closely related species	1615:1641	The low DNA-DNA hybridization values, physiological and biochemical differences, such as growth at 4 °C, acid production from inositol, lack of α-chymotrypsin activity, no casein hydrolysis, and negative for acid production from d-fructose, melibiose and sucrose, could distinguish strain RA17T from its closely related species.
30307388	1	69	theme	mountain	50:57	arg1	soil					64:67	mountain area soil	50:67	mountain area soil	50:67	nov., isolated from mountain area soil.
30307388	10	70	theme	wall	1280:1283	arg1	acid					1309:1312	meso-diaminopimelic acid	1289:1312	meso-diaminopimelic acid	1289:1312	The diagnostic diamino acid of the cell wall was meso-diaminopimelic acid.
30307388	10	70	theme	wall	1280:1283	arg1	acid					1263:1266	The diagnostic diamino acid	1240:1266	The diagnostic diamino acid of the cell wall	1240:1283	The diagnostic diamino acid of the cell wall was meso-diaminopimelic acid.
30307388	11	71	from	inositol	1441:1448	arg1	4 °C					1414:1417	4 °C	1414:1417	4 °C	1414:1417	The low DNA-DNA hybridization values, physiological and biochemical differences, such as growth at 4 °C, acid production from inositol, lack of α-chymotrypsin activity, no casein hydrolysis, and negative for acid production from d-fructose, melibiose and sucrose, could distinguish strain RA17T from its closely related species.
30307388	11	71	from	inositol	1441:1448	arg1	production					1425:1434	acid production	1420:1434	acid production from inositol	1420:1448	The low DNA-DNA hybridization values, physiological and biochemical differences, such as growth at 4 °C, acid production from inositol, lack of α-chymotrypsin activity, no casein hydrolysis, and negative for acid production from d-fructose, melibiose and sucrose, could distinguish strain RA17T from its closely related species.
30307388	3	72	theme	Paenibacillus	317:329	arg1	%					357:357	97.4 %	352:357	97.4 %	352:357	Based on 16S rRNA gene sequence similarity comparisons, strain RA17T was most closely related to Paenibacillus taihuensis THMBG22T (97.4 %), Paenibacillus rhizoryzae ACCC 1ZS3-5T (97.4 %) and Paenibacillus sacheonensis DSM SY01T (96.5 %).
30307388	3	72	theme	Paenibacillus	317:329	arg1	THMBG22T					342:349	Paenibacillus taihuensis THMBG22T	317:349	Paenibacillus taihuensis THMBG22T (97.4 %)	317:358	Based on 16S rRNA gene sequence similarity comparisons, strain RA17T was most closely related to Paenibacillus taihuensis THMBG22T (97.4 %), Paenibacillus rhizoryzae ACCC 1ZS3-5T (97.4 %) and Paenibacillus sacheonensis DSM SY01T (96.5 %).
30307388	5	73	theme	strain	691:696	arg1	RA17T					698:702	strain RA17T	691:702	strain RA17T	691:702	The DNA-DNA hybridization values between strain RA17T and the two type strains, P. taihuensis THMBG22T and P. rhizoryzae ACCC 1ZS3-5T, were 36.8 and 22.9 %, respectively.
30307388	12	74	theme	Paenibacillusmontanisoli	1745:1768	arg1	sp					1770:1771	the name Paenibacillusmontanisoli sp	1736:1771	the name Paenibacillusmontanisoli sp	1736:1771	Consequently, strain RA17T represents a novel species of the genus Paenibacillus, for which the name Paenibacillusmontanisoli sp.
30307388	6	75	theme	mol 	914:917	arg1	%					918:918	52.82 mol %	908:918	52.82 mol %	908:918	Its genome size was 6.17 Mb, comprising 5677 predicted genes with a DNA G+C content of 52.82 mol %.
30307388	4	76	theme	DSM	630:632	arg1	SY01T					634:638	P. sacheonensis DSM SY01T	614:638	P. sacheonensis DSM SY01T (85.5 %)	614:647	Analysis of the rpoB gene also indicated that RA17T had the highest similarity to P. rhizoryzae ACCC 1ZS3-5T (92.3 %), P. taihuensis THMBG22T (88.4 %) and P. sacheonensis DSM SY01T (85.5 %).
30307388	4	76	theme	DSM	630:632	arg1	%					646:646	85.5 %	641:646	85.5 %	641:646	Analysis of the rpoB gene also indicated that RA17T had the highest similarity to P. rhizoryzae ACCC 1ZS3-5T (92.3 %), P. taihuensis THMBG22T (88.4 %) and P. sacheonensis DSM SY01T (85.5 %).
30307388	11	77	theme	low	1319:1321	arg1	values					1345:1350	The low DNA-DNA hybridization values	1315:1350	The low DNA-DNA hybridization values	1315:1350	The low DNA-DNA hybridization values, physiological and biochemical differences, such as growth at 4 °C, acid production from inositol, lack of α-chymotrypsin activity, no casein hydrolysis, and negative for acid production from d-fructose, melibiose and sucrose, could distinguish strain RA17T from its closely related species.
30307388	12	78	theme	Paenibacillus	1711:1723	arg1	genus					1705:1709	the genus Paenibacillus	1701:1723	the genus Paenibacillus	1701:1723	Consequently, strain RA17T represents a novel species of the genus Paenibacillus, for which the name Paenibacillusmontanisoli sp.
30307388	3	79	theme	sacheonensis	426:437	arg1	SY01T					443:447	Paenibacillus sacheonensis DSM SY01T	412:447	Paenibacillus sacheonensis DSM SY01T (96.5 %)	412:456	Based on 16S rRNA gene sequence similarity comparisons, strain RA17T was most closely related to Paenibacillus taihuensis THMBG22T (97.4 %), Paenibacillus rhizoryzae ACCC 1ZS3-5T (97.4 %) and Paenibacillus sacheonensis DSM SY01T (96.5 %).
30307388	3	79	theme	sacheonensis	426:437	arg1	%					455:455	96.5 %	450:455	96.5 %	450:455	Based on 16S rRNA gene sequence similarity comparisons, strain RA17T was most closely related to Paenibacillus taihuensis THMBG22T (97.4 %), Paenibacillus rhizoryzae ACCC 1ZS3-5T (97.4 %) and Paenibacillus sacheonensis DSM SY01T (96.5 %).
30307388	5	80	theme	DNA-DNA	654:660	arg1	values					676:681	The DNA-DNA hybridization values	650:681	The DNA-DNA hybridization values between strain RA17T and the two type strains, P. taihuensis THMBG22T and P. rhizoryzae ACCC 1ZS3-5T,	650:783	The DNA-DNA hybridization values between strain RA17T and the two type strains, P. taihuensis THMBG22T and P. rhizoryzae ACCC 1ZS3-5T, were 36.8 and 22.9 %, respectively.
30307388	5	80	theme	DNA-DNA	654:660	arg1	%					804:804	36.8 and 22.9 %	790:804	36.8 and 22.9 %	790:804	The DNA-DNA hybridization values between strain RA17T and the two type strains, P. taihuensis THMBG22T and P. rhizoryzae ACCC 1ZS3-5T, were 36.8 and 22.9 %, respectively.
30307388	9	81	theme	major	1207:1211	arg1	menaquinone					1225:1235	menaquinone 7	1225:1237	menaquinone 7	1225:1237	The major quinone was menaquinone 7.
30307388	9	81	theme	major	1207:1211	arg1	quinone					1213:1219	The major quinone	1203:1219	The major quinone	1203:1219	The major quinone was menaquinone 7.
30307388	6	82	theme	DNA	889:891	arg1	G+C content					893:903	a DNA G+C content	887:903	a DNA G+C content of 52.82 mol %	887:918	Its genome size was 6.17 Mb, comprising 5677 predicted genes with a DNA G+C content of 52.82 mol %.
30307388	3	83	theme	ACCC	386:389	arg1	1ZS3-5T					391:397	Paenibacillus rhizoryzae ACCC 1ZS3-5T	361:397	Paenibacillus rhizoryzae ACCC 1ZS3-5T (97.4 %)	361:406	Based on 16S rRNA gene sequence similarity comparisons, strain RA17T was most closely related to Paenibacillus taihuensis THMBG22T (97.4 %), Paenibacillus rhizoryzae ACCC 1ZS3-5T (97.4 %) and Paenibacillus sacheonensis DSM SY01T (96.5 %).
30307388	3	83	theme	ACCC	386:389	arg1	%					405:405	97.4 %	400:405	97.4 %	400:405	Based on 16S rRNA gene sequence similarity comparisons, strain RA17T was most closely related to Paenibacillus taihuensis THMBG22T (97.4 %), Paenibacillus rhizoryzae ACCC 1ZS3-5T (97.4 %) and Paenibacillus sacheonensis DSM SY01T (96.5 %).
30307388	13	84	theme	type	1843:1846	arg1	strain					1848:1853	the type strain	1839:1853	the type strain	1839:1853	nov. is proposed, with RA17T (=KCTC 33894T=CCTCC AB 2017053T) as the type strain.
30307388	12	85	theme	strain	1658:1663	arg1	RA17T					1665:1669	strain RA17T	1658:1669	strain RA17T	1658:1669	Consequently, strain RA17T represents a novel species of the genus Paenibacillus, for which the name Paenibacillusmontanisoli sp.
30307388	3	86	theme	Paenibacillus	361:373	arg1	1ZS3-5T					391:397	Paenibacillus rhizoryzae ACCC 1ZS3-5T	361:397	Paenibacillus rhizoryzae ACCC 1ZS3-5T (97.4 %)	361:406	Based on 16S rRNA gene sequence similarity comparisons, strain RA17T was most closely related to Paenibacillus taihuensis THMBG22T (97.4 %), Paenibacillus rhizoryzae ACCC 1ZS3-5T (97.4 %) and Paenibacillus sacheonensis DSM SY01T (96.5 %).
30307388	3	86	theme	Paenibacillus	361:373	arg1	%					405:405	97.4 %	400:405	97.4 %	400:405	Based on 16S rRNA gene sequence similarity comparisons, strain RA17T was most closely related to Paenibacillus taihuensis THMBG22T (97.4 %), Paenibacillus rhizoryzae ACCC 1ZS3-5T (97.4 %) and Paenibacillus sacheonensis DSM SY01T (96.5 %).
30307388	4	87	theme	gene	480:483	arg1	Analysis					459:466	Analysis	459:466	Analysis of the rpoB gene	459:483	Analysis of the rpoB gene also indicated that RA17T had the highest similarity to P. rhizoryzae ACCC 1ZS3-5T (92.3 %), P. taihuensis THMBG22T (88.4 %) and P. sacheonensis DSM SY01T (85.5 %).
30307388	4	88	theme	P.	541:542	arg1	1ZS3-5T					560:566	P. rhizoryzae ACCC 1ZS3-5T	541:566	P. rhizoryzae ACCC 1ZS3-5T (92.3 %)	541:575	Analysis of the rpoB gene also indicated that RA17T had the highest similarity to P. rhizoryzae ACCC 1ZS3-5T (92.3 %), P. taihuensis THMBG22T (88.4 %) and P. sacheonensis DSM SY01T (85.5 %).
30307388	4	88	theme	P.	541:542	arg1	%					574:574	92.3 %	569:574	92.3 %	569:574	Analysis of the rpoB gene also indicated that RA17T had the highest similarity to P. rhizoryzae ACCC 1ZS3-5T (92.3 %), P. taihuensis THMBG22T (88.4 %) and P. sacheonensis DSM SY01T (85.5 %).
30307388	11	89	theme	activity	1474:1481	arg1	lack					1451:1454	lack	1451:1454	lack of α-chymotrypsin activity	1451:1481	The low DNA-DNA hybridization values, physiological and biochemical differences, such as growth at 4 °C, acid production from inositol, lack of α-chymotrypsin activity, no casein hydrolysis, and negative for acid production from d-fructose, melibiose and sucrose, could distinguish strain RA17T from its closely related species.
30307388	11	89	theme	activity	1474:1481	arg1	4 °C					1414:1417	4 °C	1414:1417	4 °C	1414:1417	The low DNA-DNA hybridization values, physiological and biochemical differences, such as growth at 4 °C, acid production from inositol, lack of α-chymotrypsin activity, no casein hydrolysis, and negative for acid production from d-fructose, melibiose and sucrose, could distinguish strain RA17T from its closely related species.
30307388	4	90	theme	ACCC	555:558	arg1	1ZS3-5T					560:566	P. rhizoryzae ACCC 1ZS3-5T	541:566	P. rhizoryzae ACCC 1ZS3-5T (92.3 %)	541:575	Analysis of the rpoB gene also indicated that RA17T had the highest similarity to P. rhizoryzae ACCC 1ZS3-5T (92.3 %), P. taihuensis THMBG22T (88.4 %) and P. sacheonensis DSM SY01T (85.5 %).
30307388	4	90	theme	ACCC	555:558	arg1	%					574:574	92.3 %	569:574	92.3 %	569:574	Analysis of the rpoB gene also indicated that RA17T had the highest similarity to P. rhizoryzae ACCC 1ZS3-5T (92.3 %), P. taihuensis THMBG22T (88.4 %) and P. sacheonensis DSM SY01T (85.5 %).
30307388	2	91	theme	endospore-forming	110:126	arg1	bacterium					143:151	A Gram-stain-negative, aerobic, motile, endospore-forming and rod-shaped bacterium	70:151	A Gram-stain-negative, aerobic, motile, endospore-forming and rod-shaped bacterium	70:151	A Gram-stain-negative, aerobic, motile, endospore-forming and rod-shaped bacterium, designated RA17T, was isolated from Dafan, Hubei Province, China.
31007699	0	0	theme	Products	75:82	arg1	Components					27:36	Chemical Components	18:36	Chemical Components	18:36	The Difference of Chemical Components and Biological Activities of the Raw Products slices and the Wine Steam-Processed Product from Cistanche deserticola.
31007699	0	0	theme	Products	75:82	arg1	Activities					53:62	Biological Activities	42:62	Biological Activities	42:62	The Difference of Chemical Components and Biological Activities of the Raw Products slices and the Wine Steam-Processed Product from Cistanche deserticola.
31007699	2	1	used	used	423:426	arg2	Product					404:410	its Wine Steam-Processed Product	379:410	its Wine Steam-Processed Product (WSCD)	379:417	Both the raw product of Cistanche deserticola slices (RCD) and its Wine Steam-Processed Product (WSCD) are used clinically for different effects.
31007699	2	1	used	used	423:426	arg2	RCD					370:372	RCD	370:372	RCD	370:372	Both the raw product of Cistanche deserticola slices (RCD) and its Wine Steam-Processed Product (WSCD) are used clinically for different effects.
31007699	2	1	used	used	423:426	arg2	product					329:335	the raw product	321:335	the raw product of Cistanche deserticola slices (RCD)	321:373	Both the raw product of Cistanche deserticola slices (RCD) and its Wine Steam-Processed Product (WSCD) are used clinically for different effects.
31007699	2	1	used	used	423:426	arg2	WSCD					413:416	WSCD	413:416	WSCD	413:416	Both the raw product of Cistanche deserticola slices (RCD) and its Wine Steam-Processed Product (WSCD) are used clinically for different effects.
31007699	7	2	theme	PhGs	1062:1065	arg1	amounts					1022:1028	higher amounts	1015:1028	higher amounts of total polysaccharides, total PhGs, isoacteoside, and osmanthuside B	1015:1099	The results of quantitative analysis showed that the WSCD has higher amounts of total polysaccharides, total PhGs, isoacteoside, and osmanthuside B than RCD, while the content of 2'-acetylacteoside and acteoside decreased after the SPW.
31007699	7	2	theme	PhGs	1062:1065	arg1	polysaccharides					1039:1053	total polysaccharides	1033:1053	total polysaccharides	1033:1053	The results of quantitative analysis showed that the WSCD has higher amounts of total polysaccharides, total PhGs, isoacteoside, and osmanthuside B than RCD, while the content of 2'-acetylacteoside and acteoside decreased after the SPW.
31007699	7	2	theme	PhGs	1062:1065	arg1	PhGs					1062:1065	total PhGs	1056:1065	total PhGs	1056:1065	The results of quantitative analysis showed that the WSCD has higher amounts of total polysaccharides, total PhGs, isoacteoside, and osmanthuside B than RCD, while the content of 2'-acetylacteoside and acteoside decreased after the SPW.
31007699	7	2	theme	PhGs	1062:1065	arg1	isoacteoside					1068:1079	isoacteoside	1068:1079	isoacteoside	1068:1079	The results of quantitative analysis showed that the WSCD has higher amounts of total polysaccharides, total PhGs, isoacteoside, and osmanthuside B than RCD, while the content of 2'-acetylacteoside and acteoside decreased after the SPW.
31007699	7	2	theme	PhGs	1062:1065	arg1	B					1099:1099	osmanthuside B	1086:1099	osmanthuside B	1086:1099	The results of quantitative analysis showed that the WSCD has higher amounts of total polysaccharides, total PhGs, isoacteoside, and osmanthuside B than RCD, while the content of 2'-acetylacteoside and acteoside decreased after the SPW.
31007699	7	3	theme	analysis	981:988	arg1	results					957:963	The results	953:963	The results of quantitative analysis	953:988	The results of quantitative analysis showed that the WSCD has higher amounts of total polysaccharides, total PhGs, isoacteoside, and osmanthuside B than RCD, while the content of 2'-acetylacteoside and acteoside decreased after the SPW.
31007699	8	4	theme	WSCD	1216:1219	arg1	comparison					1194:1203	The comparison	1190:1203	The comparison of RCD and WSCD on biological activities	1190:1244	The comparison of RCD and WSCD on biological activities showed that both could restore the level of sex hormone in the model of kidney-yang deficiency and improve the antioxidant effect.
31007699	8	5	theme	antioxidant	1357:1367	arg1	effect					1369:1374	the antioxidant effect	1353:1374	the antioxidant effect	1353:1374	The comparison of RCD and WSCD on biological activities showed that both could restore the level of sex hormone in the model of kidney-yang deficiency and improve the antioxidant effect.
31007699	0	6	theme	Raw	71:73	arg1	Products					75:82	the Raw Products	67:82	the Raw Products	67:82	The Difference of Chemical Components and Biological Activities of the Raw Products slices and the Wine Steam-Processed Product from Cistanche deserticola.
31007699	4	7	used	used	692:695	arg2	analysis					646:653	The principal component analysis	622:653	The principal component analysis (PCA)	622:659	The principal component analysis (PCA) and quantitative analysis were used to study the differences of the chemical compositions.
31007699	4	7	used	used	692:695	arg2	analysis					678:685	quantitative analysis	665:685	quantitative analysis	665:685	The principal component analysis (PCA) and quantitative analysis were used to study the differences of the chemical compositions.
31007699	4	7	used	used	692:695	arg2	PCA					656:658	PCA	656:658	PCA	656:658	The principal component analysis (PCA) and quantitative analysis were used to study the differences of the chemical compositions.
31007699	7	8	theme	B	1099:1099	arg1	amounts					1022:1028	higher amounts	1015:1028	higher amounts of total polysaccharides, total PhGs, isoacteoside, and osmanthuside B	1015:1099	The results of quantitative analysis showed that the WSCD has higher amounts of total polysaccharides, total PhGs, isoacteoside, and osmanthuside B than RCD, while the content of 2'-acetylacteoside and acteoside decreased after the SPW.
31007699	7	8	theme	B	1099:1099	arg1	polysaccharides					1039:1053	total polysaccharides	1033:1053	total polysaccharides	1033:1053	The results of quantitative analysis showed that the WSCD has higher amounts of total polysaccharides, total PhGs, isoacteoside, and osmanthuside B than RCD, while the content of 2'-acetylacteoside and acteoside decreased after the SPW.
31007699	7	8	theme	B	1099:1099	arg1	PhGs					1062:1065	total PhGs	1056:1065	total PhGs	1056:1065	The results of quantitative analysis showed that the WSCD has higher amounts of total polysaccharides, total PhGs, isoacteoside, and osmanthuside B than RCD, while the content of 2'-acetylacteoside and acteoside decreased after the SPW.
31007699	7	8	theme	B	1099:1099	arg1	isoacteoside					1068:1079	isoacteoside	1068:1079	isoacteoside	1068:1079	The results of quantitative analysis showed that the WSCD has higher amounts of total polysaccharides, total PhGs, isoacteoside, and osmanthuside B than RCD, while the content of 2'-acetylacteoside and acteoside decreased after the SPW.
31007699	7	8	theme	B	1099:1099	arg1	B					1099:1099	osmanthuside B	1086:1099	osmanthuside B	1086:1099	The results of quantitative analysis showed that the WSCD has higher amounts of total polysaccharides, total PhGs, isoacteoside, and osmanthuside B than RCD, while the content of 2'-acetylacteoside and acteoside decreased after the SPW.
31007699	8	9	theme	deficiency	1330:1339	arg1	model					1309:1313	the model	1305:1313	the model of kidney-yang deficiency	1305:1339	The comparison of RCD and WSCD on biological activities showed that both could restore the level of sex hormone in the model of kidney-yang deficiency and improve the antioxidant effect.
31007699	3	10	theme	steaming	495:502	arg1	process					504:510	steaming process	495:510	steaming process with wine (SPW) from Cistanche deserticola	495:553	In this study, the influences of steaming process with wine (SPW) from Cistanche deserticola on chemical compositions and biological effects were investigated.
31007699	8	11	theme	hormone	1294:1300	arg1	level					1281:1285	the level	1277:1285	the level of sex hormone in the model of kidney-yang deficiency	1277:1339	The comparison of RCD and WSCD on biological activities showed that both could restore the level of sex hormone in the model of kidney-yang deficiency and improve the antioxidant effect.
31007699	7	12	theme	total	1056:1060	arg1	PhGs					1062:1065	total PhGs	1056:1065	total PhGs	1056:1065	The results of quantitative analysis showed that the WSCD has higher amounts of total polysaccharides, total PhGs, isoacteoside, and osmanthuside B than RCD, while the content of 2'-acetylacteoside and acteoside decreased after the SPW.
31007699	3	13	theme	process	504:510	arg1	influences					481:490	the influences	477:490	the influences of steaming process with wine (SPW) from Cistanche deserticola on chemical compositions and biological effects	477:601	In this study, the influences of steaming process with wine (SPW) from Cistanche deserticola on chemical compositions and biological effects were investigated.
31007699	1	14	theme	kidney	256:261	arg1	syndrome					274:281	kidney deficiency syndrome	256:281	kidney deficiency syndrome in China for thousands of years	256:313	As a well-known Chinese herb medicine, the Cistanche deserticola has been used for the treatment of kidney deficiency syndrome in China for thousands of years.
31007699	4	15	theme	quantitative	665:676	arg1	analysis					678:685	quantitative analysis	665:685	quantitative analysis	665:685	The principal component analysis (PCA) and quantitative analysis were used to study the differences of the chemical compositions.
31007699	7	16	theme	total	1033:1037	arg1	polysaccharides					1039:1053	total polysaccharides	1033:1053	total polysaccharides	1033:1053	The results of quantitative analysis showed that the WSCD has higher amounts of total polysaccharides, total PhGs, isoacteoside, and osmanthuside B than RCD, while the content of 2'-acetylacteoside and acteoside decreased after the SPW.
31007699	4	17	theme	chemical	729:736	arg1	compositions					738:749	the chemical compositions	725:749	the chemical compositions	725:749	The principal component analysis (PCA) and quantitative analysis were used to study the differences of the chemical compositions.
31007699	4	18	theme	component	636:644	arg1	PCA					656:658	PCA	656:658	PCA	656:658	The principal component analysis (PCA) and quantitative analysis were used to study the differences of the chemical compositions.
31007699	4	18	theme	component	636:644	arg1	analysis					646:653	The principal component analysis	622:653	The principal component analysis (PCA)	622:659	The principal component analysis (PCA) and quantitative analysis were used to study the differences of the chemical compositions.
31007699	1	19	theme	well-known	161:170	arg1	medicine					185:192	a well-known Chinese herb medicine	159:192	a well-known Chinese herb medicine	159:192	As a well-known Chinese herb medicine, the Cistanche deserticola has been used for the treatment of kidney deficiency syndrome in China for thousands of years.
31007699	1	19	theme	well-known	161:170	arg1	deserticola					209:219	the Cistanche deserticola	195:219	the Cistanche deserticola	195:219	As a well-known Chinese herb medicine, the Cistanche deserticola has been used for the treatment of kidney deficiency syndrome in China for thousands of years.
31007699	3	20	with	process	504:510	arg1	wine					517:520	wine	517:520	wine (SPW)	517:526	In this study, the influences of steaming process with wine (SPW) from Cistanche deserticola on chemical compositions and biological effects were investigated.
31007699	3	20	with	process	504:510	arg1	SPW					523:525	SPW	523:525	SPW	523:525	In this study, the influences of steaming process with wine (SPW) from Cistanche deserticola on chemical compositions and biological effects were investigated.
31007699	1	21	from	China	286:290	arg1	treatment					243:251	the treatment	239:251	the treatment of kidney deficiency syndrome in China for thousands of years	239:313	As a well-known Chinese herb medicine, the Cistanche deserticola has been used for the treatment of kidney deficiency syndrome in China for thousands of years.
31007699	7	22	theme	higher	1015:1020	arg1	amounts					1022:1028	higher amounts	1015:1028	higher amounts of total polysaccharides, total PhGs, isoacteoside, and osmanthuside B	1015:1099	The results of quantitative analysis showed that the WSCD has higher amounts of total polysaccharides, total PhGs, isoacteoside, and osmanthuside B than RCD, while the content of 2'-acetylacteoside and acteoside decreased after the SPW.
31007699	7	22	theme	higher	1015:1020	arg1	polysaccharides					1039:1053	total polysaccharides	1033:1053	total polysaccharides	1033:1053	The results of quantitative analysis showed that the WSCD has higher amounts of total polysaccharides, total PhGs, isoacteoside, and osmanthuside B than RCD, while the content of 2'-acetylacteoside and acteoside decreased after the SPW.
31007699	7	22	theme	higher	1015:1020	arg1	PhGs					1062:1065	total PhGs	1056:1065	total PhGs	1056:1065	The results of quantitative analysis showed that the WSCD has higher amounts of total polysaccharides, total PhGs, isoacteoside, and osmanthuside B than RCD, while the content of 2'-acetylacteoside and acteoside decreased after the SPW.
31007699	7	22	theme	higher	1015:1020	arg1	isoacteoside					1068:1079	isoacteoside	1068:1079	isoacteoside	1068:1079	The results of quantitative analysis showed that the WSCD has higher amounts of total polysaccharides, total PhGs, isoacteoside, and osmanthuside B than RCD, while the content of 2'-acetylacteoside and acteoside decreased after the SPW.
31007699	7	22	theme	higher	1015:1020	arg1	B					1099:1099	osmanthuside B	1086:1099	osmanthuside B	1086:1099	The results of quantitative analysis showed that the WSCD has higher amounts of total polysaccharides, total PhGs, isoacteoside, and osmanthuside B than RCD, while the content of 2'-acetylacteoside and acteoside decreased after the SPW.
31007699	10	23	theme	chemical	1510:1517	arg1	components					1519:1528	its chemical components	1506:1528	its chemical components	1506:1528	The results indicated that SPW changed its chemical components and enhanced its biological activities.
31007699	1	24	theme	Chinese	172:178	arg1	medicine					185:192	a well-known Chinese herb medicine	159:192	a well-known Chinese herb medicine	159:192	As a well-known Chinese herb medicine, the Cistanche deserticola has been used for the treatment of kidney deficiency syndrome in China for thousands of years.
31007699	1	24	theme	Chinese	172:178	arg1	deserticola					209:219	the Cistanche deserticola	195:219	the Cistanche deserticola	195:219	As a well-known Chinese herb medicine, the Cistanche deserticola has been used for the treatment of kidney deficiency syndrome in China for thousands of years.
31007699	9	25	theme	kidney	1439:1444	arg1	weight					1429:1434	the viscera weight	1417:1434	the viscera weight of kidney and seminal vesicle	1417:1464	The WSCD were much better in increasing the viscera weight of kidney and seminal vesicle.
31007699	2	26	theme	different	443:451	arg1	effects					453:459	different effects	443:459	different effects	443:459	Both the raw product of Cistanche deserticola slices (RCD) and its Wine Steam-Processed Product (WSCD) are used clinically for different effects.
31007699	8	27	theme	sex	1290:1292	arg1	hormone					1294:1300	sex hormone	1290:1300	sex hormone	1290:1300	The comparison of RCD and WSCD on biological activities showed that both could restore the level of sex hormone in the model of kidney-yang deficiency and improve the antioxidant effect.
31007699	1	28	theme	herb	180:183	arg1	medicine					185:192	a well-known Chinese herb medicine	159:192	a well-known Chinese herb medicine	159:192	As a well-known Chinese herb medicine, the Cistanche deserticola has been used for the treatment of kidney deficiency syndrome in China for thousands of years.
31007699	1	28	theme	herb	180:183	arg1	deserticola					209:219	the Cistanche deserticola	195:219	the Cistanche deserticola	195:219	As a well-known Chinese herb medicine, the Cistanche deserticola has been used for the treatment of kidney deficiency syndrome in China for thousands of years.
31007699	5	29	theme	nourishing	767:776	arg1	kidney					778:783	nourishing kidney	767:783	nourishing kidney	767:783	The effects of nourishing kidney were also investigated to compare the differences between the RCD and the WSCD.
31007699	1	30	theme	deficiency	263:272	arg1	syndrome					274:281	kidney deficiency syndrome	256:281	kidney deficiency syndrome in China for thousands of years	256:313	As a well-known Chinese herb medicine, the Cistanche deserticola has been used for the treatment of kidney deficiency syndrome in China for thousands of years.
31007699	1	31	from	syndrome	274:281	arg1	China					286:290	China	286:290	China	286:290	As a well-known Chinese herb medicine, the Cistanche deserticola has been used for the treatment of kidney deficiency syndrome in China for thousands of years.
31007699	2	32	theme	slices	362:367	arg1	WSCD					413:416	WSCD	413:416	WSCD	413:416	Both the raw product of Cistanche deserticola slices (RCD) and its Wine Steam-Processed Product (WSCD) are used clinically for different effects.
31007699	2	32	theme	slices	362:367	arg1	RCD					370:372	RCD	370:372	RCD	370:372	Both the raw product of Cistanche deserticola slices (RCD) and its Wine Steam-Processed Product (WSCD) are used clinically for different effects.
31007699	2	32	theme	slices	362:367	arg1	Product					404:410	its Wine Steam-Processed Product	379:410	its Wine Steam-Processed Product (WSCD)	379:417	Both the raw product of Cistanche deserticola slices (RCD) and its Wine Steam-Processed Product (WSCD) are used clinically for different effects.
31007699	2	32	theme	slices	362:367	arg1	product					329:335	the raw product	321:335	the raw product of Cistanche deserticola slices (RCD)	321:373	Both the raw product of Cistanche deserticola slices (RCD) and its Wine Steam-Processed Product (WSCD) are used clinically for different effects.
31007699	0	33	theme	Chemical	18:25	arg1	Components					27:36	Chemical Components	18:36	Chemical Components	18:36	The Difference of Chemical Components and Biological Activities of the Raw Products slices and the Wine Steam-Processed Product from Cistanche deserticola.
31007699	1	34	theme	syndrome	274:281	arg1	treatment					243:251	the treatment	239:251	the treatment of kidney deficiency syndrome in China for thousands of years	239:313	As a well-known Chinese herb medicine, the Cistanche deserticola has been used for the treatment of kidney deficiency syndrome in China for thousands of years.
31007699	2	35	theme	Steam-Processed	388:402	arg1	WSCD					413:416	WSCD	413:416	WSCD	413:416	Both the raw product of Cistanche deserticola slices (RCD) and its Wine Steam-Processed Product (WSCD) are used clinically for different effects.
31007699	2	35	theme	Steam-Processed	388:402	arg1	Product					404:410	its Wine Steam-Processed Product	379:410	its Wine Steam-Processed Product (WSCD)	379:417	Both the raw product of Cistanche deserticola slices (RCD) and its Wine Steam-Processed Product (WSCD) are used clinically for different effects.
31007699	0	36	theme	Steam-Processed	104:118	arg1	Product					120:126	and the Wine Steam-Processed Product	91:126	Product	120:126	The Difference of Chemical Components and Biological Activities of the Raw Products slices and the Wine Steam-Processed Product from Cistanche deserticola.
31007699	7	37	contain	has	1011:1013	arg2	amounts					1022:1028	higher amounts	1015:1028	higher amounts of total polysaccharides, total PhGs, isoacteoside, and osmanthuside B	1015:1099	The results of quantitative analysis showed that the WSCD has higher amounts of total polysaccharides, total PhGs, isoacteoside, and osmanthuside B than RCD, while the content of 2'-acetylacteoside and acteoside decreased after the SPW.
31007699	7	37	contain	has	1011:1013	arg2	polysaccharides					1039:1053	total polysaccharides	1033:1053	total polysaccharides	1033:1053	The results of quantitative analysis showed that the WSCD has higher amounts of total polysaccharides, total PhGs, isoacteoside, and osmanthuside B than RCD, while the content of 2'-acetylacteoside and acteoside decreased after the SPW.
31007699	7	37	contain	has	1011:1013	arg2	isoacteoside					1068:1079	isoacteoside	1068:1079	isoacteoside	1068:1079	The results of quantitative analysis showed that the WSCD has higher amounts of total polysaccharides, total PhGs, isoacteoside, and osmanthuside B than RCD, while the content of 2'-acetylacteoside and acteoside decreased after the SPW.
31007699	7	37	contain	has	1011:1013	arg2	PhGs					1062:1065	total PhGs	1056:1065	total PhGs	1056:1065	The results of quantitative analysis showed that the WSCD has higher amounts of total polysaccharides, total PhGs, isoacteoside, and osmanthuside B than RCD, while the content of 2'-acetylacteoside and acteoside decreased after the SPW.
31007699	7	37	contain	has	1011:1013	arg2	B					1099:1099	osmanthuside B	1086:1099	osmanthuside B	1086:1099	The results of quantitative analysis showed that the WSCD has higher amounts of total polysaccharides, total PhGs, isoacteoside, and osmanthuside B than RCD, while the content of 2'-acetylacteoside and acteoside decreased after the SPW.
31007699	7	37	contain	has	1011:1013	arg1	WSCD					1006:1009	the WSCD	1002:1009	the WSCD	1002:1009	The results of quantitative analysis showed that the WSCD has higher amounts of total polysaccharides, total PhGs, isoacteoside, and osmanthuside B than RCD, while the content of 2'-acetylacteoside and acteoside decreased after the SPW.
31007699	6	38	theme	PCA	869:871	arg1	results					873:879	The PCA results	865:879	The PCA results	865:879	The PCA results indicated that the obvious separation was achieved in the RCD and WSCD.
31007699	2	39	theme	Wine	383:386	arg1	WSCD					413:416	WSCD	413:416	WSCD	413:416	Both the raw product of Cistanche deserticola slices (RCD) and its Wine Steam-Processed Product (WSCD) are used clinically for different effects.
31007699	2	39	theme	Wine	383:386	arg1	Product					404:410	its Wine Steam-Processed Product	379:410	its Wine Steam-Processed Product (WSCD)	379:417	Both the raw product of Cistanche deserticola slices (RCD) and its Wine Steam-Processed Product (WSCD) are used clinically for different effects.
31007699	10	40	theme	biological	1547:1556	arg1	activities					1558:1567	its biological activities	1543:1567	its biological activities	1543:1567	The results indicated that SPW changed its chemical components and enhanced its biological activities.
31007699	8	41	theme	RCD	1208:1210	arg1	comparison					1194:1203	The comparison	1190:1203	The comparison of RCD and WSCD on biological activities	1190:1244	The comparison of RCD and WSCD on biological activities showed that both could restore the level of sex hormone in the model of kidney-yang deficiency and improve the antioxidant effect.
31007699	3	42	theme	Cistanche	533:541	arg1	deserticola					543:553	Cistanche deserticola	533:553	Cistanche deserticola	533:553	In this study, the influences of steaming process with wine (SPW) from Cistanche deserticola on chemical compositions and biological effects were investigated.
31007699	3	43	from	deserticola	543:553	arg1	influences					481:490	the influences	477:490	the influences of steaming process with wine (SPW) from Cistanche deserticola on chemical compositions and biological effects	477:601	In this study, the influences of steaming process with wine (SPW) from Cistanche deserticola on chemical compositions and biological effects were investigated.
31007699	3	43	from	deserticola	543:553	arg1	process					504:510	steaming process	495:510	steaming process with wine (SPW) from Cistanche deserticola	495:553	In this study, the influences of steaming process with wine (SPW) from Cistanche deserticola on chemical compositions and biological effects were investigated.
31007699	1	44	theme	Cistanche	199:207	arg1	medicine					185:192	a well-known Chinese herb medicine	159:192	a well-known Chinese herb medicine	159:192	As a well-known Chinese herb medicine, the Cistanche deserticola has been used for the treatment of kidney deficiency syndrome in China for thousands of years.
31007699	1	44	theme	Cistanche	199:207	arg1	deserticola					209:219	the Cistanche deserticola	195:219	the Cistanche deserticola	195:219	As a well-known Chinese herb medicine, the Cistanche deserticola has been used for the treatment of kidney deficiency syndrome in China for thousands of years.
31007699	0	45	theme	Components	27:36	arg1	Difference					4:13	The Difference	0:13	The Difference of Chemical Components and Biological Activities of the Raw Products	0:82	The Difference of Chemical Components and Biological Activities of the Raw Products slices and the Wine Steam-Processed Product from Cistanche deserticola.
31007699	6	46	theme	obvious	900:906	arg1	separation					908:917	the obvious separation	896:917	the obvious separation	896:917	The PCA results indicated that the obvious separation was achieved in the RCD and WSCD.
31007699	1	47	from	treatment	243:251	arg1	China					286:290	China	286:290	China	286:290	As a well-known Chinese herb medicine, the Cistanche deserticola has been used for the treatment of kidney deficiency syndrome in China for thousands of years.
31007699	3	48	theme	biological	584:593	arg1	effects					595:601	biological effects	584:601	biological effects	584:601	In this study, the influences of steaming process with wine (SPW) from Cistanche deserticola on chemical compositions and biological effects were investigated.
31007699	5	49	theme	kidney	778:783	arg1	effects					756:762	The effects	752:762	The effects of nourishing kidney	752:783	The effects of nourishing kidney were also investigated to compare the differences between the RCD and the WSCD.
31007699	0	50	theme	Activities	53:62	arg1	Difference					4:13	The Difference	0:13	The Difference of Chemical Components and Biological Activities of the Raw Products	0:82	The Difference of Chemical Components and Biological Activities of the Raw Products slices and the Wine Steam-Processed Product from Cistanche deserticola.
31007699	8	51	theme	kidney-yang	1318:1328	arg1	deficiency					1330:1339	kidney-yang deficiency	1318:1339	kidney-yang deficiency	1318:1339	The comparison of RCD and WSCD on biological activities showed that both could restore the level of sex hormone in the model of kidney-yang deficiency and improve the antioxidant effect.
31007699	2	52	theme	raw	325:327	arg1	RCD					370:372	RCD	370:372	RCD	370:372	Both the raw product of Cistanche deserticola slices (RCD) and its Wine Steam-Processed Product (WSCD) are used clinically for different effects.
31007699	2	52	theme	raw	325:327	arg1	product					329:335	the raw product	321:335	the raw product of Cistanche deserticola slices (RCD)	321:373	Both the raw product of Cistanche deserticola slices (RCD) and its Wine Steam-Processed Product (WSCD) are used clinically for different effects.
31007699	0	53	theme	Biological	42:51	arg1	Activities					53:62	Biological Activities	42:62	Biological Activities	42:62	The Difference of Chemical Components and Biological Activities of the Raw Products slices and the Wine Steam-Processed Product from Cistanche deserticola.
31007699	9	54	theme	seminal	1450:1456	arg1	vesicle					1458:1464	seminal vesicle	1450:1464	seminal vesicle	1450:1464	The WSCD were much better in increasing the viscera weight of kidney and seminal vesicle.
31007699	7	55	theme	polysaccharides	1039:1053	arg1	amounts					1022:1028	higher amounts	1015:1028	higher amounts of total polysaccharides, total PhGs, isoacteoside, and osmanthuside B	1015:1099	The results of quantitative analysis showed that the WSCD has higher amounts of total polysaccharides, total PhGs, isoacteoside, and osmanthuside B than RCD, while the content of 2'-acetylacteoside and acteoside decreased after the SPW.
31007699	7	55	theme	polysaccharides	1039:1053	arg1	polysaccharides					1039:1053	total polysaccharides	1033:1053	total polysaccharides	1033:1053	The results of quantitative analysis showed that the WSCD has higher amounts of total polysaccharides, total PhGs, isoacteoside, and osmanthuside B than RCD, while the content of 2'-acetylacteoside and acteoside decreased after the SPW.
31007699	7	55	theme	polysaccharides	1039:1053	arg1	PhGs					1062:1065	total PhGs	1056:1065	total PhGs	1056:1065	The results of quantitative analysis showed that the WSCD has higher amounts of total polysaccharides, total PhGs, isoacteoside, and osmanthuside B than RCD, while the content of 2'-acetylacteoside and acteoside decreased after the SPW.
31007699	7	55	theme	polysaccharides	1039:1053	arg1	isoacteoside					1068:1079	isoacteoside	1068:1079	isoacteoside	1068:1079	The results of quantitative analysis showed that the WSCD has higher amounts of total polysaccharides, total PhGs, isoacteoside, and osmanthuside B than RCD, while the content of 2'-acetylacteoside and acteoside decreased after the SPW.
31007699	7	55	theme	polysaccharides	1039:1053	arg1	B					1099:1099	osmanthuside B	1086:1099	osmanthuside B	1086:1099	The results of quantitative analysis showed that the WSCD has higher amounts of total polysaccharides, total PhGs, isoacteoside, and osmanthuside B than RCD, while the content of 2'-acetylacteoside and acteoside decreased after the SPW.
31007699	4	56	theme	principal	626:634	arg1	PCA					656:658	PCA	656:658	PCA	656:658	The principal component analysis (PCA) and quantitative analysis were used to study the differences of the chemical compositions.
31007699	4	56	theme	principal	626:634	arg1	analysis					646:653	The principal component analysis	622:653	The principal component analysis (PCA)	622:659	The principal component analysis (PCA) and quantitative analysis were used to study the differences of the chemical compositions.
31007699	8	57	from	level	1281:1285	arg1	model					1309:1313	the model	1305:1313	the model of kidney-yang deficiency	1305:1339	The comparison of RCD and WSCD on biological activities showed that both could restore the level of sex hormone in the model of kidney-yang deficiency and improve the antioxidant effect.
31007699	1	58	theme	years	309:313	arg1	thousands					296:304	thousands	296:304	thousands of years	296:313	As a well-known Chinese herb medicine, the Cistanche deserticola has been used for the treatment of kidney deficiency syndrome in China for thousands of years.
31007699	7	59	theme	quantitative	968:979	arg1	analysis					981:988	quantitative analysis	968:988	quantitative analysis	968:988	The results of quantitative analysis showed that the WSCD has higher amounts of total polysaccharides, total PhGs, isoacteoside, and osmanthuside B than RCD, while the content of 2'-acetylacteoside and acteoside decreased after the SPW.
31007699	1	60	used	used	230:233	arg2	deserticola					209:219	the Cistanche deserticola	195:219	the Cistanche deserticola	195:219	As a well-known Chinese herb medicine, the Cistanche deserticola has been used for the treatment of kidney deficiency syndrome in China for thousands of years.
31007699	1	60	used	used	230:233	arg2	medicine					185:192	a well-known Chinese herb medicine	159:192	a well-known Chinese herb medicine	159:192	As a well-known Chinese herb medicine, the Cistanche deserticola has been used for the treatment of kidney deficiency syndrome in China for thousands of years.
31007699	8	61	theme	biological	1224:1233	arg1	activities					1235:1244	biological activities	1224:1244	biological activities	1224:1244	The comparison of RCD and WSCD on biological activities showed that both could restore the level of sex hormone in the model of kidney-yang deficiency and improve the antioxidant effect.
31007699	7	62	theme	2'-acetylacteoside	1132:1149	arg1	content					1121:1127	the content	1117:1127	the content of 2'-acetylacteoside and acteoside	1117:1163	The results of quantitative analysis showed that the WSCD has higher amounts of total polysaccharides, total PhGs, isoacteoside, and osmanthuside B than RCD, while the content of 2'-acetylacteoside and acteoside decreased after the SPW.
31007699	4	63	theme	compositions	738:749	arg1	differences					710:720	the differences	706:720	the differences of the chemical compositions	706:749	The principal component analysis (PCA) and quantitative analysis were used to study the differences of the chemical compositions.
31007699	9	64	theme	viscera	1421:1427	arg1	weight					1429:1434	the viscera weight	1417:1434	the viscera weight of kidney and seminal vesicle	1417:1464	The WSCD were much better in increasing the viscera weight of kidney and seminal vesicle.
31007699	7	65	theme	acteoside	1155:1163	arg1	content					1121:1127	the content	1117:1127	the content of 2'-acetylacteoside and acteoside	1117:1163	The results of quantitative analysis showed that the WSCD has higher amounts of total polysaccharides, total PhGs, isoacteoside, and osmanthuside B than RCD, while the content of 2'-acetylacteoside and acteoside decreased after the SPW.
31007699	2	66	theme	deserticola	350:360	arg1	slices					362:367	Cistanche deserticola slices	340:367	Cistanche deserticola slices	340:367	Both the raw product of Cistanche deserticola slices (RCD) and its Wine Steam-Processed Product (WSCD) are used clinically for different effects.
31007699	7	67	theme	osmanthuside	1086:1097	arg1	B					1099:1099	osmanthuside B	1086:1099	osmanthuside B	1086:1099	The results of quantitative analysis showed that the WSCD has higher amounts of total polysaccharides, total PhGs, isoacteoside, and osmanthuside B than RCD, while the content of 2'-acetylacteoside and acteoside decreased after the SPW.
31007699	9	68	theme	vesicle	1458:1464	arg1	weight					1429:1434	the viscera weight	1417:1434	the viscera weight of kidney and seminal vesicle	1417:1464	The WSCD were much better in increasing the viscera weight of kidney and seminal vesicle.
31007699	7	69	theme	isoacteoside	1068:1079	arg1	amounts					1022:1028	higher amounts	1015:1028	higher amounts of total polysaccharides, total PhGs, isoacteoside, and osmanthuside B	1015:1099	The results of quantitative analysis showed that the WSCD has higher amounts of total polysaccharides, total PhGs, isoacteoside, and osmanthuside B than RCD, while the content of 2'-acetylacteoside and acteoside decreased after the SPW.
31007699	7	69	theme	isoacteoside	1068:1079	arg1	polysaccharides					1039:1053	total polysaccharides	1033:1053	total polysaccharides	1033:1053	The results of quantitative analysis showed that the WSCD has higher amounts of total polysaccharides, total PhGs, isoacteoside, and osmanthuside B than RCD, while the content of 2'-acetylacteoside and acteoside decreased after the SPW.
31007699	7	69	theme	isoacteoside	1068:1079	arg1	PhGs					1062:1065	total PhGs	1056:1065	total PhGs	1056:1065	The results of quantitative analysis showed that the WSCD has higher amounts of total polysaccharides, total PhGs, isoacteoside, and osmanthuside B than RCD, while the content of 2'-acetylacteoside and acteoside decreased after the SPW.
31007699	7	69	theme	isoacteoside	1068:1079	arg1	isoacteoside					1068:1079	isoacteoside	1068:1079	isoacteoside	1068:1079	The results of quantitative analysis showed that the WSCD has higher amounts of total polysaccharides, total PhGs, isoacteoside, and osmanthuside B than RCD, while the content of 2'-acetylacteoside and acteoside decreased after the SPW.
31007699	7	69	theme	isoacteoside	1068:1079	arg1	B					1099:1099	osmanthuside B	1086:1099	osmanthuside B	1086:1099	The results of quantitative analysis showed that the WSCD has higher amounts of total polysaccharides, total PhGs, isoacteoside, and osmanthuside B than RCD, while the content of 2'-acetylacteoside and acteoside decreased after the SPW.
31007699	6	70	dep	RCD	939:941	arg1	the					935:937	the	935:937	the	935:937	The PCA results indicated that the obvious separation was achieved in the RCD and WSCD.
31007699	3	71	theme	chemical	558:565	arg1	compositions					567:578	chemical compositions	558:578	chemical compositions	558:578	In this study, the influences of steaming process with wine (SPW) from Cistanche deserticola on chemical compositions and biological effects were investigated.
31007699	8	72	from	comparison	1194:1203	arg1	activities					1235:1244	biological activities	1224:1244	biological activities	1224:1244	The comparison of RCD and WSCD on biological activities showed that both could restore the level of sex hormone in the model of kidney-yang deficiency and improve the antioxidant effect.
31007699	2	73	theme	Cistanche	340:348	arg1	slices					362:367	Cistanche deserticola slices	340:367	Cistanche deserticola slices	340:367	Both the raw product of Cistanche deserticola slices (RCD) and its Wine Steam-Processed Product (WSCD) are used clinically for different effects.
31007699	3	74	from	influences	481:490	arg1	deserticola					543:553	Cistanche deserticola	533:553	Cistanche deserticola	533:553	In this study, the influences of steaming process with wine (SPW) from Cistanche deserticola on chemical compositions and biological effects were investigated.
31007699	3	74	from	influences	481:490	arg1	compositions					567:578	chemical compositions	558:578	chemical compositions	558:578	In this study, the influences of steaming process with wine (SPW) from Cistanche deserticola on chemical compositions and biological effects were investigated.
31007699	3	74	from	influences	481:490	arg1	effects					595:601	biological effects	584:601	biological effects	584:601	In this study, the influences of steaming process with wine (SPW) from Cistanche deserticola on chemical compositions and biological effects were investigated.
30717553	7	0	theme	ABTS⁺	1418:1422	arg1	method					1424:1429	ABTS⁺ method	1418:1429	ABTS⁺ method	1418:1429	Moreover, ABTS⁺ method showed a low response to the compositions of different fractions, so this method may not be suitable for evaluation of Prunellae Spica antioxidant capacities, while DPPH evaluation method was more suitable for TSC and TPC.
30717553	5	1	dep	analysis	988:995	arg1	results					1042:1048	results	1042:1048	results	1042:1048	Hierarchical clustering analysis (HCA) and principal component analysis (PCA) results indicated the information from chemical compositions and antioxidant capacity can represent the "differences" of different fractions.
30717553	5	2	from	compositions	1090:1101	arg1	information					1064:1074	the information	1060:1074	the information from chemical compositions and antioxidant capacity	1060:1126	Hierarchical clustering analysis (HCA) and principal component analysis (PCA) results indicated the information from chemical compositions and antioxidant capacity can represent the "differences" of different fractions.
30717553	3	3	theme	Spica	693:697	arg1	ingredients					641:651	multiple pharmaceutical ingredients	617:651	multiple pharmaceutical ingredients	617:651	Then the multivariate statistical method was adopted to analyze the relationship between the multiple pharmaceutical ingredients and antioxidant capacities of Prunellae Spica.
30717553	3	3	theme	Spica	693:697	arg1	capacities					669:678	antioxidant capacities	657:678	antioxidant capacities	657:678	Then the multivariate statistical method was adopted to analyze the relationship between the multiple pharmaceutical ingredients and antioxidant capacities of Prunellae Spica.
30717553	5	4	theme	differences	1147:1157	arg1	"					1158:1158	the "differences"	1142:1158	the "differences" of different fractions	1142:1181	Hierarchical clustering analysis (HCA) and principal component analysis (PCA) results indicated the information from chemical compositions and antioxidant capacity can represent the "differences" of different fractions.
30717553	6	5	theme	correlation	1194:1204	arg1	CCorA					1216:1220	CCorA	1216:1220	CCorA	1216:1220	Canonical correlation analysis (CCorA) revealed a high positive correlation between the amounts of multiple chemical compositions and the antioxidant capacities (r=0.970 0), and the first canonical variate had been reached.
30717553	6	5	theme	correlation	1194:1204	arg1	analysis					1206:1213	Canonical correlation analysis	1184:1213	Canonical correlation analysis (CCorA)	1184:1221	Canonical correlation analysis (CCorA) revealed a high positive correlation between the amounts of multiple chemical compositions and the antioxidant capacities (r=0.970 0), and the first canonical variate had been reached.
30717553	1	6	theme	medicinal	182:190	arg1	plant					192:196	a perennial edible and medicinal plant	159:196	a perennial edible and medicinal plant	159:196	Prunellae Spica is a perennial edible and medicinal plant, rich in antioxidant substances.
30717553	1	6	theme	medicinal	182:190	arg1	Spica					150:154	Prunellae Spica	140:154	Prunellae Spica	140:154	Prunellae Spica is a perennial edible and medicinal plant, rich in antioxidant substances.
30717553	8	7	theme	food	1803:1806	arg1	field					1794:1798	the field	1790:1798	the field of food or medicine	1790:1818	The results of this study have important reference significance for the evaluation method on antioxidant activity of Prunellae Spica in the field of food or medicine as well as for the development of related extracts.
30717553	7	8	theme	Spica	1560:1564	arg1	capacities					1578:1587	Prunellae Spica antioxidant capacities	1550:1587	Prunellae Spica antioxidant capacities	1550:1587	Moreover, ABTS⁺ method showed a low response to the compositions of different fractions, so this method may not be suitable for evaluation of Prunellae Spica antioxidant capacities, while DPPH evaluation method was more suitable for TSC and TPC.
30717553	8	9	theme	medicine	1811:1818	arg1	field					1794:1798	the field	1790:1798	the field of food or medicine	1790:1818	The results of this study have important reference significance for the evaluation method on antioxidant activity of Prunellae Spica in the field of food or medicine as well as for the development of related extracts.
30717553	3	10	theme	multivariate	533:544	arg1	method					558:563	Then the multivariate statistical method	524:563	Then the multivariate statistical method	524:563	Then the multivariate statistical method was adopted to analyze the relationship between the multiple pharmaceutical ingredients and antioxidant capacities of Prunellae Spica.
30717553	4	11	theme	TSC	838:840	arg1	concentration					767:779	relatively high concentration	751:779	relatively high concentration of TFC (0.61±0.10) g·g⁻¹DW, TPC (0.52±0.09) g·g⁻¹DW, and TSC (0.21±0.03) g·g⁻¹DW	751:860	The results showed that ethyl acetate fraction had relatively high concentration of TFC (0.61±0.10) g·g⁻¹DW, TPC (0.52±0.09) g·g⁻¹DW, and TSC (0.21±0.03) g·g⁻¹DW, with high scavenging capacity of DPPH (3.1±0.38) mmol·L⁻¹·g⁻¹DW and FRAP (2.56±0.35) mmol·L⁻¹·g⁻¹DW.
30717553	8	12	from	method	1737:1742	arg1	activity					1759:1766	antioxidant activity	1747:1766	antioxidant activity of Prunellae Spica in the field of food or medicine	1747:1818	The results of this study have important reference significance for the evaluation method on antioxidant activity of Prunellae Spica in the field of food or medicine as well as for the development of related extracts.
30717553	6	13	theme	positive	1239:1246	arg1	correlation					1248:1258	a high positive correlation	1232:1258	a high positive correlation between the amounts of multiple chemical compositions and the antioxidant capacities (r=0.970 0)	1232:1355	Canonical correlation analysis (CCorA) revealed a high positive correlation between the amounts of multiple chemical compositions and the antioxidant capacities (r=0.970 0), and the first canonical variate had been reached.
30717553	5	14	theme	Hierarchical	964:975	arg1	analysis					988:995	Hierarchical clustering analysis	964:995	Hierarchical clustering analysis (HCA)	964:1001	Hierarchical clustering analysis (HCA) and principal component analysis (PCA) results indicated the information from chemical compositions and antioxidant capacity can represent the "differences" of different fractions.
30717553	5	14	theme	Hierarchical	964:975	arg1	HCA					998:1000	HCA	998:1000	HCA	998:1000	Hierarchical clustering analysis (HCA) and principal component analysis (PCA) results indicated the information from chemical compositions and antioxidant capacity can represent the "differences" of different fractions.
30717553	1	15	from	rich	199:202	arg1	substances					219:228	antioxidant substances	207:228	antioxidant substances	207:228	Prunellae Spica is a perennial edible and medicinal plant, rich in antioxidant substances.
30717553	2	16	from	fractions	441:449	arg1	Spica					485:489	Spica	485:489	Spica	485:489	Total flavonoids (TFC), Phenolics (TPC), triterpenoids (TSC), polysaccharides (PC) and their antioxidant capacities (by the FRAP, DPPH and ABTS⁺ methods) of ethyl acetate fraction, n-butanol fraction and other fractions of aqueous extract from Prunellae Spica were investigated in this study.
30717553	4	17	contain	had	747:749	arg1	fraction					738:745	ethyl acetate fraction	724:745	ethyl acetate fraction	724:745	The results showed that ethyl acetate fraction had relatively high concentration of TFC (0.61±0.10) g·g⁻¹DW, TPC (0.52±0.09) g·g⁻¹DW, and TSC (0.21±0.03) g·g⁻¹DW, with high scavenging capacity of DPPH (3.1±0.38) mmol·L⁻¹·g⁻¹DW and FRAP (2.56±0.35) mmol·L⁻¹·g⁻¹DW.
30717553	4	17	contain	had	747:749	arg2	concentration					767:779	relatively high concentration	751:779	relatively high concentration of TFC (0.61±0.10) g·g⁻¹DW, TPC (0.52±0.09) g·g⁻¹DW, and TSC (0.21±0.03) g·g⁻¹DW	751:860	The results showed that ethyl acetate fraction had relatively high concentration of TFC (0.61±0.10) g·g⁻¹DW, TPC (0.52±0.09) g·g⁻¹DW, and TSC (0.21±0.03) g·g⁻¹DW, with high scavenging capacity of DPPH (3.1±0.38) mmol·L⁻¹·g⁻¹DW and FRAP (2.56±0.35) mmol·L⁻¹·g⁻¹DW.
30717553	8	18	theme	important	1685:1693	arg1	significance					1705:1716	important reference significance	1685:1716	important reference significance	1685:1716	The results of this study have important reference significance for the evaluation method on antioxidant activity of Prunellae Spica in the field of food or medicine as well as for the development of related extracts.
30717553	2	19	theme	other	435:439	arg1	fractions					441:449	other fractions	435:449	other fractions	435:449	Total flavonoids (TFC), Phenolics (TPC), triterpenoids (TSC), polysaccharides (PC) and their antioxidant capacities (by the FRAP, DPPH and ABTS⁺ methods) of ethyl acetate fraction, n-butanol fraction and other fractions of aqueous extract from Prunellae Spica were investigated in this study.
30717553	8	20	theme	related	1854:1860	arg1	extracts					1862:1869	related extracts	1854:1869	related extracts	1854:1869	The results of this study have important reference significance for the evaluation method on antioxidant activity of Prunellae Spica in the field of food or medicine as well as for the development of related extracts.
30717553	4	21	theme	mmol·L⁻¹·g⁻¹DW	948:961	arg1	capacity					884:891	high scavenging capacity	868:891	high scavenging capacity of DPPH (3.1±0.38) mmol·L⁻¹·g⁻¹DW and FRAP (2.56±0.35) mmol·L⁻¹·g⁻¹DW	868:961	The results showed that ethyl acetate fraction had relatively high concentration of TFC (0.61±0.10) g·g⁻¹DW, TPC (0.52±0.09) g·g⁻¹DW, and TSC (0.21±0.03) g·g⁻¹DW, with high scavenging capacity of DPPH (3.1±0.38) mmol·L⁻¹·g⁻¹DW and FRAP (2.56±0.35) mmol·L⁻¹·g⁻¹DW.
30717553	2	22	theme	fraction	422:429	arg1	TSC					287:289	TSC	287:289	TSC	287:289	Total flavonoids (TFC), Phenolics (TPC), triterpenoids (TSC), polysaccharides (PC) and their antioxidant capacities (by the FRAP, DPPH and ABTS⁺ methods) of ethyl acetate fraction, n-butanol fraction and other fractions of aqueous extract from Prunellae Spica were investigated in this study.
30717553	2	22	theme	fraction	422:429	arg1	flavonoids					237:246	Total flavonoids	231:246	Total flavonoids (TFC)	231:252	Total flavonoids (TFC), Phenolics (TPC), triterpenoids (TSC), polysaccharides (PC) and their antioxidant capacities (by the FRAP, DPPH and ABTS⁺ methods) of ethyl acetate fraction, n-butanol fraction and other fractions of aqueous extract from Prunellae Spica were investigated in this study.
30717553	2	22	theme	fraction	422:429	arg1	TPC					266:268	TPC	266:268	TPC	266:268	Total flavonoids (TFC), Phenolics (TPC), triterpenoids (TSC), polysaccharides (PC) and their antioxidant capacities (by the FRAP, DPPH and ABTS⁺ methods) of ethyl acetate fraction, n-butanol fraction and other fractions of aqueous extract from Prunellae Spica were investigated in this study.
30717553	2	22	theme	fraction	422:429	arg1	polysaccharides					293:307	polysaccharides	293:307	polysaccharides (PC)	293:312	Total flavonoids (TFC), Phenolics (TPC), triterpenoids (TSC), polysaccharides (PC) and their antioxidant capacities (by the FRAP, DPPH and ABTS⁺ methods) of ethyl acetate fraction, n-butanol fraction and other fractions of aqueous extract from Prunellae Spica were investigated in this study.
30717553	2	22	theme	fraction	422:429	arg1	TFC					249:251	TFC	249:251	TFC	249:251	Total flavonoids (TFC), Phenolics (TPC), triterpenoids (TSC), polysaccharides (PC) and their antioxidant capacities (by the FRAP, DPPH and ABTS⁺ methods) of ethyl acetate fraction, n-butanol fraction and other fractions of aqueous extract from Prunellae Spica were investigated in this study.
30717553	2	22	theme	fraction	422:429	arg1	capacities					336:345	their antioxidant capacities	318:345	their antioxidant capacities (by the FRAP, DPPH and ABTS⁺ methods) of ethyl acetate fraction, n-butanol fraction and other fractions of aqueous extract from Prunellae Spica	318:489	Total flavonoids (TFC), Phenolics (TPC), triterpenoids (TSC), polysaccharides (PC) and their antioxidant capacities (by the FRAP, DPPH and ABTS⁺ methods) of ethyl acetate fraction, n-butanol fraction and other fractions of aqueous extract from Prunellae Spica were investigated in this study.
30717553	2	22	theme	fraction	422:429	arg1	PC					310:311	PC	310:311	PC	310:311	Total flavonoids (TFC), Phenolics (TPC), triterpenoids (TSC), polysaccharides (PC) and their antioxidant capacities (by the FRAP, DPPH and ABTS⁺ methods) of ethyl acetate fraction, n-butanol fraction and other fractions of aqueous extract from Prunellae Spica were investigated in this study.
30717553	2	22	theme	fraction	422:429	arg1	triterpenoids					272:284	triterpenoids	272:284	triterpenoids (TSC)	272:290	Total flavonoids (TFC), Phenolics (TPC), triterpenoids (TSC), polysaccharides (PC) and their antioxidant capacities (by the FRAP, DPPH and ABTS⁺ methods) of ethyl acetate fraction, n-butanol fraction and other fractions of aqueous extract from Prunellae Spica were investigated in this study.
30717553	2	22	theme	fraction	422:429	arg1	Phenolics					255:263	Phenolics	255:263	Phenolics (TPC)	255:269	Total flavonoids (TFC), Phenolics (TPC), triterpenoids (TSC), polysaccharides (PC) and their antioxidant capacities (by the FRAP, DPPH and ABTS⁺ methods) of ethyl acetate fraction, n-butanol fraction and other fractions of aqueous extract from Prunellae Spica were investigated in this study.
30717553	0	23	theme	Spica	89:93	arg1	ingredients					37:47	multiple pharmaceutical ingredients	13:47	multiple pharmaceutical ingredients	13:47	[Analysis on multiple pharmaceutical ingredients and antioxidant capacities of Prunellae Spica based on multivariate statistical analysis].
30717553	0	23	theme	Spica	89:93	arg1	capacities					65:74	antioxidant capacities	53:74	antioxidant capacities of Prunellae Spica	53:93	[Analysis on multiple pharmaceutical ingredients and antioxidant capacities of Prunellae Spica based on multivariate statistical analysis].
30717553	1	24	theme	edible	171:176	arg1	plant					192:196	a perennial edible and medicinal plant	159:196	a perennial edible and medicinal plant	159:196	Prunellae Spica is a perennial edible and medicinal plant, rich in antioxidant substances.
30717553	1	24	theme	edible	171:176	arg1	Spica					150:154	Prunellae Spica	140:154	Prunellae Spica	140:154	Prunellae Spica is a perennial edible and medicinal plant, rich in antioxidant substances.
30717553	4	25	theme	scavenging	873:882	arg1	capacity					884:891	high scavenging capacity	868:891	high scavenging capacity of DPPH (3.1±0.38) mmol·L⁻¹·g⁻¹DW and FRAP (2.56±0.35) mmol·L⁻¹·g⁻¹DW	868:961	The results showed that ethyl acetate fraction had relatively high concentration of TFC (0.61±0.10) g·g⁻¹DW, TPC (0.52±0.09) g·g⁻¹DW, and TSC (0.21±0.03) g·g⁻¹DW, with high scavenging capacity of DPPH (3.1±0.38) mmol·L⁻¹·g⁻¹DW and FRAP (2.56±0.35) mmol·L⁻¹·g⁻¹DW.
30717553	0	26	from	[Analysis	0:8	arg1	ingredients					37:47	multiple pharmaceutical ingredients	13:47	multiple pharmaceutical ingredients	13:47	[Analysis on multiple pharmaceutical ingredients and antioxidant capacities of Prunellae Spica based on multivariate statistical analysis].
30717553	0	26	from	[Analysis	0:8	arg1	capacities					65:74	antioxidant capacities	53:74	antioxidant capacities of Prunellae Spica	53:93	[Analysis on multiple pharmaceutical ingredients and antioxidant capacities of Prunellae Spica based on multivariate statistical analysis].
30717553	4	27	theme	acetate	730:736	arg1	fraction					738:745	ethyl acetate fraction	724:745	ethyl acetate fraction	724:745	The results showed that ethyl acetate fraction had relatively high concentration of TFC (0.61±0.10) g·g⁻¹DW, TPC (0.52±0.09) g·g⁻¹DW, and TSC (0.21±0.03) g·g⁻¹DW, with high scavenging capacity of DPPH (3.1±0.38) mmol·L⁻¹·g⁻¹DW and FRAP (2.56±0.35) mmol·L⁻¹·g⁻¹DW.
30717553	6	28	theme	compositions	1301:1312	arg1	compositions					1301:1312	multiple chemical compositions	1283:1312	multiple chemical compositions	1283:1312	Canonical correlation analysis (CCorA) revealed a high positive correlation between the amounts of multiple chemical compositions and the antioxidant capacities (r=0.970 0), and the first canonical variate had been reached.
30717553	6	28	theme	compositions	1301:1312	arg1	capacities					1334:1343	the antioxidant capacities	1318:1343	the antioxidant capacities (r=0.970 0)	1318:1355	Canonical correlation analysis (CCorA) revealed a high positive correlation between the amounts of multiple chemical compositions and the antioxidant capacities (r=0.970 0), and the first canonical variate had been reached.
30717553	6	28	theme	compositions	1301:1312	arg1	r=0.970					1346:1352	r=0.970 0	1346:1354	r=0.970 0	1346:1354	Canonical correlation analysis (CCorA) revealed a high positive correlation between the amounts of multiple chemical compositions and the antioxidant capacities (r=0.970 0), and the first canonical variate had been reached.
30717553	6	28	theme	compositions	1301:1312	arg1	amounts					1272:1278	the amounts	1268:1278	the amounts of multiple chemical compositions	1268:1312	Canonical correlation analysis (CCorA) revealed a high positive correlation between the amounts of multiple chemical compositions and the antioxidant capacities (r=0.970 0), and the first canonical variate had been reached.
30717553	5	29	theme	component	1017:1025	arg1	PCA					1037:1039	PCA	1037:1039	PCA	1037:1039	Hierarchical clustering analysis (HCA) and principal component analysis (PCA) results indicated the information from chemical compositions and antioxidant capacity can represent the "differences" of different fractions.
30717553	5	29	theme	component	1017:1025	arg1	analysis					1027:1034	principal component analysis	1007:1034	principal component analysis (PCA)	1007:1040	Hierarchical clustering analysis (HCA) and principal component analysis (PCA) results indicated the information from chemical compositions and antioxidant capacity can represent the "differences" of different fractions.
30717553	7	30	theme	different	1476:1484	arg1	fractions					1486:1494	different fractions	1476:1494	different fractions	1476:1494	Moreover, ABTS⁺ method showed a low response to the compositions of different fractions, so this method may not be suitable for evaluation of Prunellae Spica antioxidant capacities, while DPPH evaluation method was more suitable for TSC and TPC.
30717553	0	31	theme	statistical	117:127	arg1	analysis					129:136	multivariate statistical analysis	104:136	multivariate statistical analysis	104:136	[Analysis on multiple pharmaceutical ingredients and antioxidant capacities of Prunellae Spica based on multivariate statistical analysis].
30717553	8	32	theme	antioxidant	1747:1757	arg1	activity					1759:1766	antioxidant activity	1747:1766	antioxidant activity of Prunellae Spica in the field of food or medicine	1747:1818	The results of this study have important reference significance for the evaluation method on antioxidant activity of Prunellae Spica in the field of food or medicine as well as for the development of related extracts.
30717553	7	33	theme	DPPH	1596:1599	arg1	method					1612:1617	DPPH evaluation method	1596:1617	DPPH evaluation method	1596:1617	Moreover, ABTS⁺ method showed a low response to the compositions of different fractions, so this method may not be suitable for evaluation of Prunellae Spica antioxidant capacities, while DPPH evaluation method was more suitable for TSC and TPC.
30717553	2	34	from	fraction	402:409	arg1	Spica					485:489	Spica	485:489	Spica	485:489	Total flavonoids (TFC), Phenolics (TPC), triterpenoids (TSC), polysaccharides (PC) and their antioxidant capacities (by the FRAP, DPPH and ABTS⁺ methods) of ethyl acetate fraction, n-butanol fraction and other fractions of aqueous extract from Prunellae Spica were investigated in this study.
30717553	6	35	theme	first	1366:1370	arg1	variate					1382:1388	the first canonical variate	1362:1388	the first canonical variate	1362:1388	Canonical correlation analysis (CCorA) revealed a high positive correlation between the amounts of multiple chemical compositions and the antioxidant capacities (r=0.970 0), and the first canonical variate had been reached.
30717553	3	36	dep	ingredients	641:651	arg1	the					613:615	the	613:615	the	613:615	Then the multivariate statistical method was adopted to analyze the relationship between the multiple pharmaceutical ingredients and antioxidant capacities of Prunellae Spica.
30717553	0	37	theme	multiple	13:20	arg1	ingredients					37:47	multiple pharmaceutical ingredients	13:47	multiple pharmaceutical ingredients	13:47	[Analysis on multiple pharmaceutical ingredients and antioxidant capacities of Prunellae Spica based on multivariate statistical analysis].
30717553	8	38	contain	have	1680:1683	arg2	significance					1705:1716	important reference significance	1685:1716	important reference significance	1685:1716	The results of this study have important reference significance for the evaluation method on antioxidant activity of Prunellae Spica in the field of food or medicine as well as for the development of related extracts.
30717553	8	38	contain	have	1680:1683	arg1	results					1658:1664	The results	1654:1664	The results of this study	1654:1678	The results of this study have important reference significance for the evaluation method on antioxidant activity of Prunellae Spica in the field of food or medicine as well as for the development of related extracts.
30717553	7	39	theme	antioxidant	1566:1576	arg1	capacities					1578:1587	Prunellae Spica antioxidant capacities	1550:1587	Prunellae Spica antioxidant capacities	1550:1587	Moreover, ABTS⁺ method showed a low response to the compositions of different fractions, so this method may not be suitable for evaluation of Prunellae Spica antioxidant capacities, while DPPH evaluation method was more suitable for TSC and TPC.
30717553	4	40	theme	mmol·L⁻¹·g⁻¹DW	912:925	arg1	capacity					884:891	high scavenging capacity	868:891	high scavenging capacity of DPPH (3.1±0.38) mmol·L⁻¹·g⁻¹DW and FRAP (2.56±0.35) mmol·L⁻¹·g⁻¹DW	868:961	The results showed that ethyl acetate fraction had relatively high concentration of TFC (0.61±0.10) g·g⁻¹DW, TPC (0.52±0.09) g·g⁻¹DW, and TSC (0.21±0.03) g·g⁻¹DW, with high scavenging capacity of DPPH (3.1±0.38) mmol·L⁻¹·g⁻¹DW and FRAP (2.56±0.35) mmol·L⁻¹·g⁻¹DW.
30717553	2	41	theme	acetate	394:400	arg1	fraction					402:409	ethyl acetate fraction	388:409	ethyl acetate fraction	388:409	Total flavonoids (TFC), Phenolics (TPC), triterpenoids (TSC), polysaccharides (PC) and their antioxidant capacities (by the FRAP, DPPH and ABTS⁺ methods) of ethyl acetate fraction, n-butanol fraction and other fractions of aqueous extract from Prunellae Spica were investigated in this study.
30717553	0	42	theme	antioxidant	53:63	arg1	capacities					65:74	antioxidant capacities	53:74	antioxidant capacities of Prunellae Spica	53:93	[Analysis on multiple pharmaceutical ingredients and antioxidant capacities of Prunellae Spica based on multivariate statistical analysis].
30717553	5	43	theme	chemical	1081:1088	arg1	compositions					1090:1101	chemical compositions	1081:1101	chemical compositions	1081:1101	Hierarchical clustering analysis (HCA) and principal component analysis (PCA) results indicated the information from chemical compositions and antioxidant capacity can represent the "differences" of different fractions.
30717553	3	44	theme	pharmaceutical	626:639	arg1	ingredients					641:651	multiple pharmaceutical ingredients	617:651	multiple pharmaceutical ingredients	617:651	Then the multivariate statistical method was adopted to analyze the relationship between the multiple pharmaceutical ingredients and antioxidant capacities of Prunellae Spica.
30717553	4	45	theme	high	762:765	arg1	concentration					767:779	relatively high concentration	751:779	relatively high concentration of TFC (0.61±0.10) g·g⁻¹DW, TPC (0.52±0.09) g·g⁻¹DW, and TSC (0.21±0.03) g·g⁻¹DW	751:860	The results showed that ethyl acetate fraction had relatively high concentration of TFC (0.61±0.10) g·g⁻¹DW, TPC (0.52±0.09) g·g⁻¹DW, and TSC (0.21±0.03) g·g⁻¹DW, with high scavenging capacity of DPPH (3.1±0.38) mmol·L⁻¹·g⁻¹DW and FRAP (2.56±0.35) mmol·L⁻¹·g⁻¹DW.
30717553	2	46	theme	antioxidant	324:334	arg1	capacities					336:345	their antioxidant capacities	318:345	their antioxidant capacities (by the FRAP, DPPH and ABTS⁺ methods) of ethyl acetate fraction, n-butanol fraction and other fractions of aqueous extract from Prunellae Spica	318:489	Total flavonoids (TFC), Phenolics (TPC), triterpenoids (TSC), polysaccharides (PC) and their antioxidant capacities (by the FRAP, DPPH and ABTS⁺ methods) of ethyl acetate fraction, n-butanol fraction and other fractions of aqueous extract from Prunellae Spica were investigated in this study.
30717553	5	47	theme	different	1163:1171	arg1	fractions					1173:1181	different fractions	1163:1181	different fractions	1163:1181	Hierarchical clustering analysis (HCA) and principal component analysis (PCA) results indicated the information from chemical compositions and antioxidant capacity can represent the "differences" of different fractions.
30717553	2	48	from	Spica	485:489	arg1	fraction					402:409	ethyl acetate fraction	388:409	ethyl acetate fraction	388:409	Total flavonoids (TFC), Phenolics (TPC), triterpenoids (TSC), polysaccharides (PC) and their antioxidant capacities (by the FRAP, DPPH and ABTS⁺ methods) of ethyl acetate fraction, n-butanol fraction and other fractions of aqueous extract from Prunellae Spica were investigated in this study.
30717553	2	48	from	Spica	485:489	arg1	fraction					422:429	n-butanol fraction	412:429	n-butanol fraction	412:429	Total flavonoids (TFC), Phenolics (TPC), triterpenoids (TSC), polysaccharides (PC) and their antioxidant capacities (by the FRAP, DPPH and ABTS⁺ methods) of ethyl acetate fraction, n-butanol fraction and other fractions of aqueous extract from Prunellae Spica were investigated in this study.
30717553	2	48	from	Spica	485:489	arg1	fractions					441:449	other fractions	435:449	other fractions	435:449	Total flavonoids (TFC), Phenolics (TPC), triterpenoids (TSC), polysaccharides (PC) and their antioxidant capacities (by the FRAP, DPPH and ABTS⁺ methods) of ethyl acetate fraction, n-butanol fraction and other fractions of aqueous extract from Prunellae Spica were investigated in this study.
30717553	2	48	from	Spica	485:489	arg1	extract					462:468	aqueous extract	454:468	aqueous extract from Prunellae Spica	454:489	Total flavonoids (TFC), Phenolics (TPC), triterpenoids (TSC), polysaccharides (PC) and their antioxidant capacities (by the FRAP, DPPH and ABTS⁺ methods) of ethyl acetate fraction, n-butanol fraction and other fractions of aqueous extract from Prunellae Spica were investigated in this study.
30717553	8	49	theme	Spica	1781:1785	arg1	activity					1759:1766	antioxidant activity	1747:1766	antioxidant activity of Prunellae Spica in the field of food or medicine	1747:1818	The results of this study have important reference significance for the evaluation method on antioxidant activity of Prunellae Spica in the field of food or medicine as well as for the development of related extracts.
30717553	8	50	theme	study	1674:1678	arg1	results					1658:1664	The results	1654:1664	The results of this study	1654:1678	The results of this study have important reference significance for the evaluation method on antioxidant activity of Prunellae Spica in the field of food or medicine as well as for the development of related extracts.
30717553	5	51	from	capacity	1119:1126	arg1	information					1064:1074	the information	1060:1074	the information from chemical compositions and antioxidant capacity	1060:1126	Hierarchical clustering analysis (HCA) and principal component analysis (PCA) results indicated the information from chemical compositions and antioxidant capacity can represent the "differences" of different fractions.
30717553	2	52	theme	ethyl	388:392	arg1	fraction					402:409	ethyl acetate fraction	388:409	ethyl acetate fraction	388:409	Total flavonoids (TFC), Phenolics (TPC), triterpenoids (TSC), polysaccharides (PC) and their antioxidant capacities (by the FRAP, DPPH and ABTS⁺ methods) of ethyl acetate fraction, n-butanol fraction and other fractions of aqueous extract from Prunellae Spica were investigated in this study.
30717553	6	53	theme	Canonical	1184:1192	arg1	CCorA					1216:1220	CCorA	1216:1220	CCorA	1216:1220	Canonical correlation analysis (CCorA) revealed a high positive correlation between the amounts of multiple chemical compositions and the antioxidant capacities (r=0.970 0), and the first canonical variate had been reached.
30717553	6	53	theme	Canonical	1184:1192	arg1	analysis					1206:1213	Canonical correlation analysis	1184:1213	Canonical correlation analysis (CCorA)	1184:1221	Canonical correlation analysis (CCorA) revealed a high positive correlation between the amounts of multiple chemical compositions and the antioxidant capacities (r=0.970 0), and the first canonical variate had been reached.
30717553	4	54	theme	TPC	809:811	arg1	concentration					767:779	relatively high concentration	751:779	relatively high concentration of TFC (0.61±0.10) g·g⁻¹DW, TPC (0.52±0.09) g·g⁻¹DW, and TSC (0.21±0.03) g·g⁻¹DW	751:860	The results showed that ethyl acetate fraction had relatively high concentration of TFC (0.61±0.10) g·g⁻¹DW, TPC (0.52±0.09) g·g⁻¹DW, and TSC (0.21±0.03) g·g⁻¹DW, with high scavenging capacity of DPPH (3.1±0.38) mmol·L⁻¹·g⁻¹DW and FRAP (2.56±0.35) mmol·L⁻¹·g⁻¹DW.
30717553	1	55	theme	rich	199:202	arg1	plant					192:196	a perennial edible and medicinal plant	159:196	a perennial edible and medicinal plant	159:196	Prunellae Spica is a perennial edible and medicinal plant, rich in antioxidant substances.
30717553	1	55	theme	rich	199:202	arg1	Spica					150:154	Prunellae Spica	140:154	Prunellae Spica	140:154	Prunellae Spica is a perennial edible and medicinal plant, rich in antioxidant substances.
30717553	2	56	from	fraction	422:429	arg1	Spica					485:489	Spica	485:489	Spica	485:489	Total flavonoids (TFC), Phenolics (TPC), triterpenoids (TSC), polysaccharides (PC) and their antioxidant capacities (by the FRAP, DPPH and ABTS⁺ methods) of ethyl acetate fraction, n-butanol fraction and other fractions of aqueous extract from Prunellae Spica were investigated in this study.
30717553	1	57	theme	antioxidant	207:217	arg1	substances					219:228	antioxidant substances	207:228	antioxidant substances	207:228	Prunellae Spica is a perennial edible and medicinal plant, rich in antioxidant substances.
30717553	2	58	theme	extract	462:468	arg1	fraction					402:409	ethyl acetate fraction	388:409	ethyl acetate fraction	388:409	Total flavonoids (TFC), Phenolics (TPC), triterpenoids (TSC), polysaccharides (PC) and their antioxidant capacities (by the FRAP, DPPH and ABTS⁺ methods) of ethyl acetate fraction, n-butanol fraction and other fractions of aqueous extract from Prunellae Spica were investigated in this study.
30717553	2	58	theme	extract	462:468	arg1	fraction					422:429	n-butanol fraction	412:429	n-butanol fraction	412:429	Total flavonoids (TFC), Phenolics (TPC), triterpenoids (TSC), polysaccharides (PC) and their antioxidant capacities (by the FRAP, DPPH and ABTS⁺ methods) of ethyl acetate fraction, n-butanol fraction and other fractions of aqueous extract from Prunellae Spica were investigated in this study.
30717553	2	58	theme	extract	462:468	arg1	fractions					441:449	other fractions	435:449	other fractions	435:449	Total flavonoids (TFC), Phenolics (TPC), triterpenoids (TSC), polysaccharides (PC) and their antioxidant capacities (by the FRAP, DPPH and ABTS⁺ methods) of ethyl acetate fraction, n-butanol fraction and other fractions of aqueous extract from Prunellae Spica were investigated in this study.
30717553	5	59	theme	principal	1007:1015	arg1	PCA					1037:1039	PCA	1037:1039	PCA	1037:1039	Hierarchical clustering analysis (HCA) and principal component analysis (PCA) results indicated the information from chemical compositions and antioxidant capacity can represent the "differences" of different fractions.
30717553	5	59	theme	principal	1007:1015	arg1	analysis					1027:1034	principal component analysis	1007:1034	principal component analysis (PCA)	1007:1040	Hierarchical clustering analysis (HCA) and principal component analysis (PCA) results indicated the information from chemical compositions and antioxidant capacity can represent the "differences" of different fractions.
30717553	2	60	theme	Total	231:235	arg1	flavonoids					237:246	Total flavonoids	231:246	Total flavonoids (TFC)	231:252	Total flavonoids (TFC), Phenolics (TPC), triterpenoids (TSC), polysaccharides (PC) and their antioxidant capacities (by the FRAP, DPPH and ABTS⁺ methods) of ethyl acetate fraction, n-butanol fraction and other fractions of aqueous extract from Prunellae Spica were investigated in this study.
30717553	2	60	theme	Total	231:235	arg1	TFC					249:251	TFC	249:251	TFC	249:251	Total flavonoids (TFC), Phenolics (TPC), triterpenoids (TSC), polysaccharides (PC) and their antioxidant capacities (by the FRAP, DPPH and ABTS⁺ methods) of ethyl acetate fraction, n-butanol fraction and other fractions of aqueous extract from Prunellae Spica were investigated in this study.
30717553	1	61	from	substances	219:228	arg1	rich					199:202	rich	199:202	rich	199:202	Prunellae Spica is a perennial edible and medicinal plant, rich in antioxidant substances.
30717553	6	62	theme	high	1234:1237	arg1	correlation					1248:1258	a high positive correlation	1232:1258	a high positive correlation between the amounts of multiple chemical compositions and the antioxidant capacities (r=0.970 0)	1232:1355	Canonical correlation analysis (CCorA) revealed a high positive correlation between the amounts of multiple chemical compositions and the antioxidant capacities (r=0.970 0), and the first canonical variate had been reached.
30717553	5	63	theme	clustering	977:986	arg1	analysis					988:995	Hierarchical clustering analysis	964:995	Hierarchical clustering analysis (HCA)	964:1001	Hierarchical clustering analysis (HCA) and principal component analysis (PCA) results indicated the information from chemical compositions and antioxidant capacity can represent the "differences" of different fractions.
30717553	5	63	theme	clustering	977:986	arg1	HCA					998:1000	HCA	998:1000	HCA	998:1000	Hierarchical clustering analysis (HCA) and principal component analysis (PCA) results indicated the information from chemical compositions and antioxidant capacity can represent the "differences" of different fractions.
30717553	1	64	theme	Prunellae	140:148	arg1	plant					192:196	a perennial edible and medicinal plant	159:196	a perennial edible and medicinal plant	159:196	Prunellae Spica is a perennial edible and medicinal plant, rich in antioxidant substances.
30717553	1	64	theme	Prunellae	140:148	arg1	Spica					150:154	Prunellae Spica	140:154	Prunellae Spica	140:154	Prunellae Spica is a perennial edible and medicinal plant, rich in antioxidant substances.
30717553	8	65	theme	extracts	1862:1869	arg1	development					1839:1849	the development	1835:1849	the development of related extracts	1835:1869	The results of this study have important reference significance for the evaluation method on antioxidant activity of Prunellae Spica in the field of food or medicine as well as for the development of related extracts.
30717553	3	66	theme	statistical	546:556	arg1	method					558:563	Then the multivariate statistical method	524:563	Then the multivariate statistical method	524:563	Then the multivariate statistical method was adopted to analyze the relationship between the multiple pharmaceutical ingredients and antioxidant capacities of Prunellae Spica.
30717553	4	67	theme	DPPH	896:899	arg1	mmol·L⁻¹·g⁻¹DW					912:925	DPPH (3.1±0.38) mmol·L⁻¹·g⁻¹DW	896:925	DPPH (3.1±0.38) mmol·L⁻¹·g⁻¹DW	896:925	The results showed that ethyl acetate fraction had relatively high concentration of TFC (0.61±0.10) g·g⁻¹DW, TPC (0.52±0.09) g·g⁻¹DW, and TSC (0.21±0.03) g·g⁻¹DW, with high scavenging capacity of DPPH (3.1±0.38) mmol·L⁻¹·g⁻¹DW and FRAP (2.56±0.35) mmol·L⁻¹·g⁻¹DW.
30717553	8	68	from	activity	1759:1766	arg1	field					1794:1798	the field	1790:1798	the field of food or medicine	1790:1818	The results of this study have important reference significance for the evaluation method on antioxidant activity of Prunellae Spica in the field of food or medicine as well as for the development of related extracts.
30717553	4	69	theme	high	868:871	arg1	capacity					884:891	high scavenging capacity	868:891	high scavenging capacity of DPPH (3.1±0.38) mmol·L⁻¹·g⁻¹DW and FRAP (2.56±0.35) mmol·L⁻¹·g⁻¹DW	868:961	The results showed that ethyl acetate fraction had relatively high concentration of TFC (0.61±0.10) g·g⁻¹DW, TPC (0.52±0.09) g·g⁻¹DW, and TSC (0.21±0.03) g·g⁻¹DW, with high scavenging capacity of DPPH (3.1±0.38) mmol·L⁻¹·g⁻¹DW and FRAP (2.56±0.35) mmol·L⁻¹·g⁻¹DW.
30717553	6	70	theme	antioxidant	1322:1332	arg1	r=0.970					1346:1352	r=0.970 0	1346:1354	r=0.970 0	1346:1354	Canonical correlation analysis (CCorA) revealed a high positive correlation between the amounts of multiple chemical compositions and the antioxidant capacities (r=0.970 0), and the first canonical variate had been reached.
30717553	6	70	theme	antioxidant	1322:1332	arg1	capacities					1334:1343	the antioxidant capacities	1318:1343	the antioxidant capacities (r=0.970 0)	1318:1355	Canonical correlation analysis (CCorA) revealed a high positive correlation between the amounts of multiple chemical compositions and the antioxidant capacities (r=0.970 0), and the first canonical variate had been reached.
30717553	8	71	theme	reference	1695:1703	arg1	significance					1705:1716	important reference significance	1685:1716	important reference significance	1685:1716	The results of this study have important reference significance for the evaluation method on antioxidant activity of Prunellae Spica in the field of food or medicine as well as for the development of related extracts.
30717553	1	72	theme	perennial	161:169	arg1	plant					192:196	a perennial edible and medicinal plant	159:196	a perennial edible and medicinal plant	159:196	Prunellae Spica is a perennial edible and medicinal plant, rich in antioxidant substances.
30717553	1	72	theme	perennial	161:169	arg1	Spica					150:154	Prunellae Spica	140:154	Prunellae Spica	140:154	Prunellae Spica is a perennial edible and medicinal plant, rich in antioxidant substances.
30717553	7	73	theme	fractions	1486:1494	arg1	compositions					1460:1471	the compositions	1456:1471	the compositions of different fractions	1456:1494	Moreover, ABTS⁺ method showed a low response to the compositions of different fractions, so this method may not be suitable for evaluation of Prunellae Spica antioxidant capacities, while DPPH evaluation method was more suitable for TSC and TPC.
30717553	2	74	theme	n-butanol	412:420	arg1	fraction					422:429	n-butanol fraction	412:429	n-butanol fraction	412:429	Total flavonoids (TFC), Phenolics (TPC), triterpenoids (TSC), polysaccharides (PC) and their antioxidant capacities (by the FRAP, DPPH and ABTS⁺ methods) of ethyl acetate fraction, n-butanol fraction and other fractions of aqueous extract from Prunellae Spica were investigated in this study.
30717553	0	75	theme	multivariate	104:115	arg1	analysis					129:136	multivariate statistical analysis	104:136	multivariate statistical analysis	104:136	[Analysis on multiple pharmaceutical ingredients and antioxidant capacities of Prunellae Spica based on multivariate statistical analysis].
30717553	2	76	theme	aqueous	454:460	arg1	extract					462:468	aqueous extract	454:468	aqueous extract from Prunellae Spica	454:489	Total flavonoids (TFC), Phenolics (TPC), triterpenoids (TSC), polysaccharides (PC) and their antioxidant capacities (by the FRAP, DPPH and ABTS⁺ methods) of ethyl acetate fraction, n-butanol fraction and other fractions of aqueous extract from Prunellae Spica were investigated in this study.
30717553	4	77	theme	ethyl	724:728	arg1	acetate					730:736	ethyl acetate	724:736	ethyl acetate fraction	724:745	The results showed that ethyl acetate fraction had relatively high concentration of TFC (0.61±0.10) g·g⁻¹DW, TPC (0.52±0.09) g·g⁻¹DW, and TSC (0.21±0.03) g·g⁻¹DW, with high scavenging capacity of DPPH (3.1±0.38) mmol·L⁻¹·g⁻¹DW and FRAP (2.56±0.35) mmol·L⁻¹·g⁻¹DW.
30717553	6	78	theme	chemical	1292:1299	arg1	compositions					1301:1312	multiple chemical compositions	1283:1312	multiple chemical compositions	1283:1312	Canonical correlation analysis (CCorA) revealed a high positive correlation between the amounts of multiple chemical compositions and the antioxidant capacities (r=0.970 0), and the first canonical variate had been reached.
30717553	8	79	theme	evaluation	1726:1735	arg1	method					1737:1742	the evaluation method	1722:1742	the evaluation method on antioxidant activity of Prunellae Spica in the field of food or medicine	1722:1818	The results of this study have important reference significance for the evaluation method on antioxidant activity of Prunellae Spica in the field of food or medicine as well as for the development of related extracts.
30717553	2	80	theme	fractions	441:449	arg1	TSC					287:289	TSC	287:289	TSC	287:289	Total flavonoids (TFC), Phenolics (TPC), triterpenoids (TSC), polysaccharides (PC) and their antioxidant capacities (by the FRAP, DPPH and ABTS⁺ methods) of ethyl acetate fraction, n-butanol fraction and other fractions of aqueous extract from Prunellae Spica were investigated in this study.
30717553	2	80	theme	fractions	441:449	arg1	flavonoids					237:246	Total flavonoids	231:246	Total flavonoids (TFC)	231:252	Total flavonoids (TFC), Phenolics (TPC), triterpenoids (TSC), polysaccharides (PC) and their antioxidant capacities (by the FRAP, DPPH and ABTS⁺ methods) of ethyl acetate fraction, n-butanol fraction and other fractions of aqueous extract from Prunellae Spica were investigated in this study.
30717553	2	80	theme	fractions	441:449	arg1	TPC					266:268	TPC	266:268	TPC	266:268	Total flavonoids (TFC), Phenolics (TPC), triterpenoids (TSC), polysaccharides (PC) and their antioxidant capacities (by the FRAP, DPPH and ABTS⁺ methods) of ethyl acetate fraction, n-butanol fraction and other fractions of aqueous extract from Prunellae Spica were investigated in this study.
30717553	2	80	theme	fractions	441:449	arg1	polysaccharides					293:307	polysaccharides	293:307	polysaccharides (PC)	293:312	Total flavonoids (TFC), Phenolics (TPC), triterpenoids (TSC), polysaccharides (PC) and their antioxidant capacities (by the FRAP, DPPH and ABTS⁺ methods) of ethyl acetate fraction, n-butanol fraction and other fractions of aqueous extract from Prunellae Spica were investigated in this study.
30717553	2	80	theme	fractions	441:449	arg1	TFC					249:251	TFC	249:251	TFC	249:251	Total flavonoids (TFC), Phenolics (TPC), triterpenoids (TSC), polysaccharides (PC) and their antioxidant capacities (by the FRAP, DPPH and ABTS⁺ methods) of ethyl acetate fraction, n-butanol fraction and other fractions of aqueous extract from Prunellae Spica were investigated in this study.
30717553	2	80	theme	fractions	441:449	arg1	capacities					336:345	their antioxidant capacities	318:345	their antioxidant capacities (by the FRAP, DPPH and ABTS⁺ methods) of ethyl acetate fraction, n-butanol fraction and other fractions of aqueous extract from Prunellae Spica	318:489	Total flavonoids (TFC), Phenolics (TPC), triterpenoids (TSC), polysaccharides (PC) and their antioxidant capacities (by the FRAP, DPPH and ABTS⁺ methods) of ethyl acetate fraction, n-butanol fraction and other fractions of aqueous extract from Prunellae Spica were investigated in this study.
30717553	2	80	theme	fractions	441:449	arg1	PC					310:311	PC	310:311	PC	310:311	Total flavonoids (TFC), Phenolics (TPC), triterpenoids (TSC), polysaccharides (PC) and their antioxidant capacities (by the FRAP, DPPH and ABTS⁺ methods) of ethyl acetate fraction, n-butanol fraction and other fractions of aqueous extract from Prunellae Spica were investigated in this study.
30717553	2	80	theme	fractions	441:449	arg1	triterpenoids					272:284	triterpenoids	272:284	triterpenoids (TSC)	272:290	Total flavonoids (TFC), Phenolics (TPC), triterpenoids (TSC), polysaccharides (PC) and their antioxidant capacities (by the FRAP, DPPH and ABTS⁺ methods) of ethyl acetate fraction, n-butanol fraction and other fractions of aqueous extract from Prunellae Spica were investigated in this study.
30717553	2	80	theme	fractions	441:449	arg1	Phenolics					255:263	Phenolics	255:263	Phenolics (TPC)	255:269	Total flavonoids (TFC), Phenolics (TPC), triterpenoids (TSC), polysaccharides (PC) and their antioxidant capacities (by the FRAP, DPPH and ABTS⁺ methods) of ethyl acetate fraction, n-butanol fraction and other fractions of aqueous extract from Prunellae Spica were investigated in this study.
30717553	6	81	theme	canonical	1372:1380	arg1	variate					1382:1388	the first canonical variate	1362:1388	the first canonical variate	1362:1388	Canonical correlation analysis (CCorA) revealed a high positive correlation between the amounts of multiple chemical compositions and the antioxidant capacities (r=0.970 0), and the first canonical variate had been reached.
30717553	7	82	theme	evaluation	1601:1610	arg1	method					1612:1617	DPPH evaluation method	1596:1617	DPPH evaluation method	1596:1617	Moreover, ABTS⁺ method showed a low response to the compositions of different fractions, so this method may not be suitable for evaluation of Prunellae Spica antioxidant capacities, while DPPH evaluation method was more suitable for TSC and TPC.
30717553	5	83	dep	indicated	1050:1058	arg1	represent					1132:1140	represent	1132:1140	indicated the information from chemical compositions and antioxidant capacity can represent the "differences" of different fractions	1050:1181	Hierarchical clustering analysis (HCA) and principal component analysis (PCA) results indicated the information from chemical compositions and antioxidant capacity can represent the "differences" of different fractions.
30717553	0	84	theme	pharmaceutical	22:35	arg1	ingredients					37:47	multiple pharmaceutical ingredients	13:47	multiple pharmaceutical ingredients	13:47	[Analysis on multiple pharmaceutical ingredients and antioxidant capacities of Prunellae Spica based on multivariate statistical analysis].
30717553	5	85	theme	antioxidant	1107:1117	arg1	capacity					1119:1126	antioxidant capacity	1107:1126	antioxidant capacity	1107:1126	Hierarchical clustering analysis (HCA) and principal component analysis (PCA) results indicated the information from chemical compositions and antioxidant capacity can represent the "differences" of different fractions.
30717553	2	86	theme	ABTS⁺	370:374	arg1	methods					376:382	ABTS⁺ methods	370:382	ABTS⁺ methods	370:382	Total flavonoids (TFC), Phenolics (TPC), triterpenoids (TSC), polysaccharides (PC) and their antioxidant capacities (by the FRAP, DPPH and ABTS⁺ methods) of ethyl acetate fraction, n-butanol fraction and other fractions of aqueous extract from Prunellae Spica were investigated in this study.
30717553	7	87	theme	capacities	1578:1587	arg1	evaluation					1536:1545	evaluation	1536:1545	evaluation of Prunellae Spica antioxidant capacities	1536:1587	Moreover, ABTS⁺ method showed a low response to the compositions of different fractions, so this method may not be suitable for evaluation of Prunellae Spica antioxidant capacities, while DPPH evaluation method was more suitable for TSC and TPC.
30717553	2	88	theme	fraction	402:409	arg1	TSC					287:289	TSC	287:289	TSC	287:289	Total flavonoids (TFC), Phenolics (TPC), triterpenoids (TSC), polysaccharides (PC) and their antioxidant capacities (by the FRAP, DPPH and ABTS⁺ methods) of ethyl acetate fraction, n-butanol fraction and other fractions of aqueous extract from Prunellae Spica were investigated in this study.
30717553	2	88	theme	fraction	402:409	arg1	flavonoids					237:246	Total flavonoids	231:246	Total flavonoids (TFC)	231:252	Total flavonoids (TFC), Phenolics (TPC), triterpenoids (TSC), polysaccharides (PC) and their antioxidant capacities (by the FRAP, DPPH and ABTS⁺ methods) of ethyl acetate fraction, n-butanol fraction and other fractions of aqueous extract from Prunellae Spica were investigated in this study.
30717553	2	88	theme	fraction	402:409	arg1	TPC					266:268	TPC	266:268	TPC	266:268	Total flavonoids (TFC), Phenolics (TPC), triterpenoids (TSC), polysaccharides (PC) and their antioxidant capacities (by the FRAP, DPPH and ABTS⁺ methods) of ethyl acetate fraction, n-butanol fraction and other fractions of aqueous extract from Prunellae Spica were investigated in this study.
30717553	2	88	theme	fraction	402:409	arg1	polysaccharides					293:307	polysaccharides	293:307	polysaccharides (PC)	293:312	Total flavonoids (TFC), Phenolics (TPC), triterpenoids (TSC), polysaccharides (PC) and their antioxidant capacities (by the FRAP, DPPH and ABTS⁺ methods) of ethyl acetate fraction, n-butanol fraction and other fractions of aqueous extract from Prunellae Spica were investigated in this study.
30717553	2	88	theme	fraction	402:409	arg1	TFC					249:251	TFC	249:251	TFC	249:251	Total flavonoids (TFC), Phenolics (TPC), triterpenoids (TSC), polysaccharides (PC) and their antioxidant capacities (by the FRAP, DPPH and ABTS⁺ methods) of ethyl acetate fraction, n-butanol fraction and other fractions of aqueous extract from Prunellae Spica were investigated in this study.
30717553	2	88	theme	fraction	402:409	arg1	capacities					336:345	their antioxidant capacities	318:345	their antioxidant capacities (by the FRAP, DPPH and ABTS⁺ methods) of ethyl acetate fraction, n-butanol fraction and other fractions of aqueous extract from Prunellae Spica	318:489	Total flavonoids (TFC), Phenolics (TPC), triterpenoids (TSC), polysaccharides (PC) and their antioxidant capacities (by the FRAP, DPPH and ABTS⁺ methods) of ethyl acetate fraction, n-butanol fraction and other fractions of aqueous extract from Prunellae Spica were investigated in this study.
30717553	2	88	theme	fraction	402:409	arg1	PC					310:311	PC	310:311	PC	310:311	Total flavonoids (TFC), Phenolics (TPC), triterpenoids (TSC), polysaccharides (PC) and their antioxidant capacities (by the FRAP, DPPH and ABTS⁺ methods) of ethyl acetate fraction, n-butanol fraction and other fractions of aqueous extract from Prunellae Spica were investigated in this study.
30717553	2	88	theme	fraction	402:409	arg1	triterpenoids					272:284	triterpenoids	272:284	triterpenoids (TSC)	272:290	Total flavonoids (TFC), Phenolics (TPC), triterpenoids (TSC), polysaccharides (PC) and their antioxidant capacities (by the FRAP, DPPH and ABTS⁺ methods) of ethyl acetate fraction, n-butanol fraction and other fractions of aqueous extract from Prunellae Spica were investigated in this study.
30717553	2	88	theme	fraction	402:409	arg1	Phenolics					255:263	Phenolics	255:263	Phenolics (TPC)	255:269	Total flavonoids (TFC), Phenolics (TPC), triterpenoids (TSC), polysaccharides (PC) and their antioxidant capacities (by the FRAP, DPPH and ABTS⁺ methods) of ethyl acetate fraction, n-butanol fraction and other fractions of aqueous extract from Prunellae Spica were investigated in this study.
30717553	4	89	theme	TFC	784:786	arg1	concentration					767:779	relatively high concentration	751:779	relatively high concentration of TFC (0.61±0.10) g·g⁻¹DW, TPC (0.52±0.09) g·g⁻¹DW, and TSC (0.21±0.03) g·g⁻¹DW	751:860	The results showed that ethyl acetate fraction had relatively high concentration of TFC (0.61±0.10) g·g⁻¹DW, TPC (0.52±0.09) g·g⁻¹DW, and TSC (0.21±0.03) g·g⁻¹DW, with high scavenging capacity of DPPH (3.1±0.38) mmol·L⁻¹·g⁻¹DW and FRAP (2.56±0.35) mmol·L⁻¹·g⁻¹DW.
30717553	6	90	theme	multiple	1283:1290	arg1	compositions					1301:1312	multiple chemical compositions	1283:1312	multiple chemical compositions	1283:1312	Canonical correlation analysis (CCorA) revealed a high positive correlation between the amounts of multiple chemical compositions and the antioxidant capacities (r=0.970 0), and the first canonical variate had been reached.
30717553	4	91	theme	FRAP	931:934	arg1	mmol·L⁻¹·g⁻¹DW					948:961	FRAP (2.56±0.35) mmol·L⁻¹·g⁻¹DW	931:961	FRAP (2.56±0.35) mmol·L⁻¹·g⁻¹DW	931:961	The results showed that ethyl acetate fraction had relatively high concentration of TFC (0.61±0.10) g·g⁻¹DW, TPC (0.52±0.09) g·g⁻¹DW, and TSC (0.21±0.03) g·g⁻¹DW, with high scavenging capacity of DPPH (3.1±0.38) mmol·L⁻¹·g⁻¹DW and FRAP (2.56±0.35) mmol·L⁻¹·g⁻¹DW.
30717553	3	92	theme	multiple	617:624	arg1	ingredients					641:651	multiple pharmaceutical ingredients	617:651	multiple pharmaceutical ingredients	617:651	Then the multivariate statistical method was adopted to analyze the relationship between the multiple pharmaceutical ingredients and antioxidant capacities of Prunellae Spica.
30717553	7	93	theme	low	1440:1442	arg1	response					1444:1451	a low response	1438:1451	a low response to the compositions of different fractions	1438:1494	Moreover, ABTS⁺ method showed a low response to the compositions of different fractions, so this method may not be suitable for evaluation of Prunellae Spica antioxidant capacities, while DPPH evaluation method was more suitable for TSC and TPC.
30717553	2	94	dep	capacities	336:345	arg1	DPPH					361:364	DPPH	361:364	DPPH	361:364	Total flavonoids (TFC), Phenolics (TPC), triterpenoids (TSC), polysaccharides (PC) and their antioxidant capacities (by the FRAP, DPPH and ABTS⁺ methods) of ethyl acetate fraction, n-butanol fraction and other fractions of aqueous extract from Prunellae Spica were investigated in this study.
30717553	2	94	dep	capacities	336:345	arg1	methods					376:382	ABTS⁺ methods	370:382	ABTS⁺ methods	370:382	Total flavonoids (TFC), Phenolics (TPC), triterpenoids (TSC), polysaccharides (PC) and their antioxidant capacities (by the FRAP, DPPH and ABTS⁺ methods) of ethyl acetate fraction, n-butanol fraction and other fractions of aqueous extract from Prunellae Spica were investigated in this study.
30717553	3	95	theme	antioxidant	657:667	arg1	capacities					669:678	antioxidant capacities	657:678	antioxidant capacities	657:678	Then the multivariate statistical method was adopted to analyze the relationship between the multiple pharmaceutical ingredients and antioxidant capacities of Prunellae Spica.
30717553	5	96	theme	fractions	1173:1181	arg1	"					1158:1158	the "differences"	1142:1158	the "differences" of different fractions	1142:1181	Hierarchical clustering analysis (HCA) and principal component analysis (PCA) results indicated the information from chemical compositions and antioxidant capacity can represent the "differences" of different fractions.
29600438	0	0	theme	Rifampicin	105:114	arg1	Pharmacokinetics					85:100	the Intracellular Pharmacokinetics	67:100	the Intracellular Pharmacokinetics of Rifampicin in Macrophages	67:129	Functionalization of PLGA Nanoparticles with 1,3-β-glucan Enhances the Intracellular Pharmacokinetics of Rifampicin in Macrophages.
29600438	7	1	theme	Intracellular	921:933	arg1	pharmacokinetics					935:950	Intracellular pharmacokinetics	921:950	Intracellular pharmacokinetics of rifampicin and NPs	921:972	Intracellular pharmacokinetics of rifampicin and NPs were evaluated in THP-1 derived macrophages.
29600438	8	2	theme	ADAPT	1111:1115	arg1	software					1119:1126	ADAPT 5 software	1111:1126	ADAPT 5 software	1111:1126	A pharmacokinetic model was developed to describe uptake, and modelling was performed using ADAPT 5 software.
29600438	7	3	theme	NPs	970:972	arg1	pharmacokinetics					935:950	Intracellular pharmacokinetics	921:950	Intracellular pharmacokinetics of rifampicin and NPs	921:972	Intracellular pharmacokinetics of rifampicin and NPs were evaluated in THP-1 derived macrophages.
29600438	4	4	theme	higher	613:618	arg1	amounts					620:626	higher amounts	613:626	higher amounts of rifampicin	613:640	We hypothesized that Glu-PLGA NPs would be taken up at a faster rate than PLGA NPs, and consequently deliver higher amounts of rifampicin into the macrophages.
29600438	4	4	theme	higher	613:618	arg1	rifampicin					631:640	rifampicin	631:640	rifampicin	631:640	We hypothesized that Glu-PLGA NPs would be taken up at a faster rate than PLGA NPs, and consequently deliver higher amounts of rifampicin into the macrophages.
29600438	12	5	theme	PLGA	1497:1500	arg1	NPs					1515:1517	the PLGA and Glu-PLGA NPs	1493:1517	NPs	1515:1517	After 24 h, the amount of rifampicin delivered by the PLGA and Glu-PLGA NPs was similar.
29600438	12	6	theme	Glu-PLGA	1506:1513	arg1	NPs					1515:1517	the PLGA and Glu-PLGA NPs	1493:1517	NPs	1515:1517	After 24 h, the amount of rifampicin delivered by the PLGA and Glu-PLGA NPs was similar.
29600438	14	7	theme	CONCLUSIONS	1609:1619	arg1	Functionalization					1621:1637	CONCLUSIONS Functionalization	1609:1637	CONCLUSIONS Functionalization of PLGA NPs with 1,3-β-glucan	1609:1667	CONCLUSIONS Functionalization of PLGA NPs with 1,3-β-glucan resulted in faster uptake of rifampicin into macrophages.
29600438	13	8	theme	10-fold	1563:1569	arg1	increase					1571:1578	at least a 10-fold increase	1552:1578	at least a 10-fold increase in the uptake of rifampicin	1552:1606	The NPs resulted in at least a 10-fold increase in the uptake of rifampicin.
29600438	7	9	theme	rifampicin	955:964	arg1	pharmacokinetics					935:950	Intracellular pharmacokinetics	921:950	Intracellular pharmacokinetics of rifampicin and NPs	921:972	Intracellular pharmacokinetics of rifampicin and NPs were evaluated in THP-1 derived macrophages.
29600438	5	10	theme	conjugate	711:719	arg1	1,3-β-glucan					721:732	conjugate 1,3-β-glucan	711:732	conjugate 1,3-β-glucan	711:732	METHODS Carbodiimide chemistry was employed to conjugate 1,3-β-glucan and rhodamine to PLGA.
29600438	6	11	theme	loaded	768:773	arg1	PLGA					775:778	Rifampicin loaded PLGA and Glu-PLGA NPs	757:795	PLGA	775:778	Rifampicin loaded PLGA and Glu-PLGA NPs as well as rhodamine functionalized PLGA and Glu-PLGA NPs were synthesized using an emulsion solvent evaporation technique.
29600438	14	12	theme	rifampicin	1698:1707	arg1	uptake					1688:1693	faster uptake	1681:1693	faster uptake of rifampicin into macrophages	1681:1724	CONCLUSIONS Functionalization of PLGA NPs with 1,3-β-glucan resulted in faster uptake of rifampicin into macrophages.
29600438	3	13	theme	intracellular	372:384	arg1	pharmacokinetics					386:401	the intracellular pharmacokinetics	368:401	the intracellular pharmacokinetics of the anti-tubercular drug rifampicin delivered by 1,3-β-glucan functionalized PLGA NPs (Glu-PLGA)	368:501	In this study we characterized the intracellular pharmacokinetics of the anti-tubercular drug rifampicin delivered by 1,3-β-glucan functionalized PLGA NPs (Glu-PLGA).
29600438	4	14	theme	Glu-PLGA	525:532	arg1	NPs					534:536	Glu-PLGA NPs	525:536	Glu-PLGA NPs	525:536	We hypothesized that Glu-PLGA NPs would be taken up at a faster rate than PLGA NPs, and consequently deliver higher amounts of rifampicin into the macrophages.
29600438	10	15	theme	NPs	1270:1272	arg1	Expulsion					1257:1265	Expulsion	1257:1265	Expulsion of NPs from the macrophages	1257:1293	Expulsion of NPs from the macrophages was also observed, which was 3 fold greater for Glu-PLGA NPs than for PLGA NPs.
29600438	5	16	theme	METHODS	664:670	arg1	chemistry					685:693	METHODS Carbodiimide chemistry	664:693	METHODS Carbodiimide chemistry	664:693	METHODS Carbodiimide chemistry was employed to conjugate 1,3-β-glucan and rhodamine to PLGA.
29600438	6	17	theme	Glu-PLGA	842:849	arg1	NPs					851:853	rhodamine functionalized PLGA and Glu-PLGA NPs	808:853	NPs	851:853	Rifampicin loaded PLGA and Glu-PLGA NPs as well as rhodamine functionalized PLGA and Glu-PLGA NPs were synthesized using an emulsion solvent evaporation technique.
29600438	15	18	theme	intracellular	1768:1780	arg1	eradication					1782:1792	rapid intracellular eradication	1762:1792	rapid intracellular eradication of Mycobacterium tuberculosis	1762:1822	These NPs may be useful to achieve rapid intracellular eradication of Mycobacterium tuberculosis.
29600438	15	19	theme	tuberculosis	1811:1822	arg1	eradication					1782:1792	rapid intracellular eradication	1762:1792	rapid intracellular eradication of Mycobacterium tuberculosis	1762:1822	These NPs may be useful to achieve rapid intracellular eradication of Mycobacterium tuberculosis.
29600438	3	20	theme	rifampicin	431:440	arg1	pharmacokinetics					386:401	the intracellular pharmacokinetics	368:401	the intracellular pharmacokinetics of the anti-tubercular drug rifampicin delivered by 1,3-β-glucan functionalized PLGA NPs (Glu-PLGA)	368:501	In this study we characterized the intracellular pharmacokinetics of the anti-tubercular drug rifampicin delivered by 1,3-β-glucan functionalized PLGA NPs (Glu-PLGA).
29600438	5	21	theme	Carbodiimide	672:683	arg1	chemistry					685:693	METHODS Carbodiimide chemistry	664:693	METHODS Carbodiimide chemistry	664:693	METHODS Carbodiimide chemistry was employed to conjugate 1,3-β-glucan and rhodamine to PLGA.
29600438	14	22	theme	NPs	1647:1649	arg1	Functionalization					1621:1637	CONCLUSIONS Functionalization	1609:1637	CONCLUSIONS Functionalization of PLGA NPs with 1,3-β-glucan	1609:1667	CONCLUSIONS Functionalization of PLGA NPs with 1,3-β-glucan resulted in faster uptake of rifampicin into macrophages.
29600438	6	23	dep	loaded	768:773	arg1	Rifampicin					757:766	Rifampicin	757:766	Rifampicin	757:766	Rifampicin loaded PLGA and Glu-PLGA NPs as well as rhodamine functionalized PLGA and Glu-PLGA NPs were synthesized using an emulsion solvent evaporation technique.
29600438	10	24	from	macrophages	1283:1293	arg1	NPs					1270:1272	NPs	1270:1272	NPs from the macrophages	1270:1293	Expulsion of NPs from the macrophages was also observed, which was 3 fold greater for Glu-PLGA NPs than for PLGA NPs.
29600438	10	24	from	macrophages	1283:1293	arg1	Expulsion					1257:1265	Expulsion	1257:1265	Expulsion of NPs from the macrophages	1257:1293	Expulsion of NPs from the macrophages was also observed, which was 3 fold greater for Glu-PLGA NPs than for PLGA NPs.
29600438	9	25	theme	uptake	1167:1172	arg1	rate					1159:1162	the rate	1155:1162	the rate of uptake of rifampicin	1155:1186	RESULTS The NPs increased the rate of uptake of rifampicin by a factor of 17 and 62 in case of PLGA and Glu-PLGA, respectively.
29600438	2	26	dep	loaded	294:299	arg1	drug					289:292	drug	289:292	drug	289:292	1,3-β-glucan has been proposed as a ligand to target drug loaded nanoparticles (NPs) to macrophages.
29600438	3	27	theme	anti-tubercular	410:424	arg1	rifampicin					431:440	the anti-tubercular drug rifampicin	406:440	the anti-tubercular drug rifampicin delivered by 1,3-β-glucan functionalized PLGA NPs (Glu-PLGA)	406:501	In this study we characterized the intracellular pharmacokinetics of the anti-tubercular drug rifampicin delivered by 1,3-β-glucan functionalized PLGA NPs (Glu-PLGA).
29600438	7	28	theme	derived	998:1004	arg1	macrophages					1006:1016	THP-1 derived macrophages	992:1016	THP-1 derived macrophages	992:1016	Intracellular pharmacokinetics of rifampicin and NPs were evaluated in THP-1 derived macrophages.
29600438	6	29	theme	functionalized	818:831	arg1	PLGA					833:836	rhodamine functionalized PLGA and Glu-PLGA NPs	808:853	PLGA	833:836	Rifampicin loaded PLGA and Glu-PLGA NPs as well as rhodamine functionalized PLGA and Glu-PLGA NPs were synthesized using an emulsion solvent evaporation technique.
29600438	15	30	theme	rapid	1762:1766	arg1	eradication					1782:1792	rapid intracellular eradication	1762:1792	rapid intracellular eradication of Mycobacterium tuberculosis	1762:1822	These NPs may be useful to achieve rapid intracellular eradication of Mycobacterium tuberculosis.
29600438	3	31	theme	drug	426:429	arg1	rifampicin					431:440	the anti-tubercular drug rifampicin	406:440	the anti-tubercular drug rifampicin delivered by 1,3-β-glucan functionalized PLGA NPs (Glu-PLGA)	406:501	In this study we characterized the intracellular pharmacokinetics of the anti-tubercular drug rifampicin delivered by 1,3-β-glucan functionalized PLGA NPs (Glu-PLGA).
29600438	1	32	theme	PURPOSE	132:138	arg1	tuberculosis					154:165	PURPOSE Mycobacterium tuberculosis	132:165	PURPOSE Mycobacterium tuberculosis	132:165	PURPOSE Mycobacterium tuberculosis which causes tuberculosis, is primarily resident within macrophages.
29600438	9	33	theme	rifampicin	1177:1186	arg1	uptake					1167:1172	uptake	1167:1172	uptake of rifampicin	1167:1186	RESULTS The NPs increased the rate of uptake of rifampicin by a factor of 17 and 62 in case of PLGA and Glu-PLGA, respectively.
29600438	10	34	theme	Glu-PLGA	1343:1350	arg1	NPs					1352:1354	Glu-PLGA NPs	1343:1354	Glu-PLGA NPs	1343:1354	Expulsion of NPs from the macrophages was also observed, which was 3 fold greater for Glu-PLGA NPs than for PLGA NPs.
29600438	3	35	theme	functionalized	468:481	arg1	Glu-PLGA					493:500	Glu-PLGA	493:500	Glu-PLGA	493:500	In this study we characterized the intracellular pharmacokinetics of the anti-tubercular drug rifampicin delivered by 1,3-β-glucan functionalized PLGA NPs (Glu-PLGA).
29600438	3	35	theme	functionalized	468:481	arg1	NPs					488:490	1,3-β-glucan functionalized PLGA NPs	455:490	1,3-β-glucan functionalized PLGA NPs (Glu-PLGA)	455:501	In this study we characterized the intracellular pharmacokinetics of the anti-tubercular drug rifampicin delivered by 1,3-β-glucan functionalized PLGA NPs (Glu-PLGA).
29600438	15	36	theme	Mycobacterium	1797:1809	arg1	tuberculosis					1811:1822	Mycobacterium tuberculosis	1797:1822	Mycobacterium tuberculosis	1797:1822	These NPs may be useful to achieve rapid intracellular eradication of Mycobacterium tuberculosis.
29600438	14	37	with	Functionalization	1621:1637	arg1	1,3-β-glucan					1656:1667	1,3-β-glucan	1656:1667	1,3-β-glucan	1656:1667	CONCLUSIONS Functionalization of PLGA NPs with 1,3-β-glucan resulted in faster uptake of rifampicin into macrophages.
29600438	8	38	theme	pharmacokinetic	1021:1035	arg1	model					1037:1041	A pharmacokinetic model	1019:1041	A pharmacokinetic model	1019:1041	A pharmacokinetic model was developed to describe uptake, and modelling was performed using ADAPT 5 software.
29600438	3	39	theme	PLGA	483:486	arg1	Glu-PLGA					493:500	Glu-PLGA	493:500	Glu-PLGA	493:500	In this study we characterized the intracellular pharmacokinetics of the anti-tubercular drug rifampicin delivered by 1,3-β-glucan functionalized PLGA NPs (Glu-PLGA).
29600438	3	39	theme	PLGA	483:486	arg1	NPs					488:490	1,3-β-glucan functionalized PLGA NPs	455:490	1,3-β-glucan functionalized PLGA NPs (Glu-PLGA)	455:501	In this study we characterized the intracellular pharmacokinetics of the anti-tubercular drug rifampicin delivered by 1,3-β-glucan functionalized PLGA NPs (Glu-PLGA).
29600438	0	40	from	Pharmacokinetics	85:100	arg1	Macrophages					119:129	Macrophages	119:129	Macrophages	119:129	Functionalization of PLGA Nanoparticles with 1,3-β-glucan Enhances the Intracellular Pharmacokinetics of Rifampicin in Macrophages.
29600438	0	41	theme	Nanoparticles	26:38	arg1	Functionalization					0:16	Functionalization	0:16	Functionalization of PLGA Nanoparticles with 1,3-β-glucan	0:56	Functionalization of PLGA Nanoparticles with 1,3-β-glucan Enhances the Intracellular Pharmacokinetics of Rifampicin in Macrophages.
29600438	6	42	theme	rhodamine	808:816	arg1	PLGA					833:836	rhodamine functionalized PLGA and Glu-PLGA NPs	808:853	PLGA	833:836	Rifampicin loaded PLGA and Glu-PLGA NPs as well as rhodamine functionalized PLGA and Glu-PLGA NPs were synthesized using an emulsion solvent evaporation technique.
29600438	9	43	theme	62	1210:1211	arg1	factor					1193:1198	a factor	1191:1198	a factor of 17 and 62	1191:1211	RESULTS The NPs increased the rate of uptake of rifampicin by a factor of 17 and 62 in case of PLGA and Glu-PLGA, respectively.
29600438	4	44	theme	faster	561:566	arg1	rate					568:571	a faster rate	559:571	a faster rate than PLGA NPs	559:585	We hypothesized that Glu-PLGA NPs would be taken up at a faster rate than PLGA NPs, and consequently deliver higher amounts of rifampicin into the macrophages.
29600438	11	45	theme	uptake	1397:1402	arg1	ratio					1388:1392	the ratio	1384:1392	the ratio of uptake to expulsion	1384:1415	However, the ratio of uptake to expulsion was similar for both NPs.
29600438	11	45	theme	uptake	1397:1402	arg1	similar					1421:1427	similar	1421:1427	similar	1421:1427	However, the ratio of uptake to expulsion was similar for both NPs.
29600438	13	46	theme	rifampicin	1597:1606	arg1	uptake					1587:1592	the uptake	1583:1592	the uptake of rifampicin	1583:1606	The NPs resulted in at least a 10-fold increase in the uptake of rifampicin.
29600438	1	47	theme	Mycobacterium	140:152	arg1	tuberculosis					154:165	PURPOSE Mycobacterium tuberculosis	132:165	PURPOSE Mycobacterium tuberculosis	132:165	PURPOSE Mycobacterium tuberculosis which causes tuberculosis, is primarily resident within macrophages.
29600438	3	48	theme	1,3-β-glucan	455:466	arg1	Glu-PLGA					493:500	Glu-PLGA	493:500	Glu-PLGA	493:500	In this study we characterized the intracellular pharmacokinetics of the anti-tubercular drug rifampicin delivered by 1,3-β-glucan functionalized PLGA NPs (Glu-PLGA).
29600438	3	48	theme	1,3-β-glucan	455:466	arg1	NPs					488:490	1,3-β-glucan functionalized PLGA NPs	455:490	1,3-β-glucan functionalized PLGA NPs (Glu-PLGA)	455:501	In this study we characterized the intracellular pharmacokinetics of the anti-tubercular drug rifampicin delivered by 1,3-β-glucan functionalized PLGA NPs (Glu-PLGA).
29600438	4	49	theme	PLGA	578:581	arg1	NPs					583:585	PLGA NPs	578:585	PLGA NPs	578:585	We hypothesized that Glu-PLGA NPs would be taken up at a faster rate than PLGA NPs, and consequently deliver higher amounts of rifampicin into the macrophages.
29600438	6	50	theme	evaporation	898:908	arg1	technique					910:918	an emulsion solvent evaporation technique	878:918	an emulsion solvent evaporation technique	878:918	Rifampicin loaded PLGA and Glu-PLGA NPs as well as rhodamine functionalized PLGA and Glu-PLGA NPs were synthesized using an emulsion solvent evaporation technique.
29600438	13	51	from	increase	1571:1578	arg1	uptake					1587:1592	the uptake	1583:1592	the uptake of rifampicin	1583:1606	The NPs resulted in at least a 10-fold increase in the uptake of rifampicin.
29600438	14	52	theme	faster	1681:1686	arg1	uptake					1688:1693	faster uptake	1681:1693	faster uptake of rifampicin into macrophages	1681:1724	CONCLUSIONS Functionalization of PLGA NPs with 1,3-β-glucan resulted in faster uptake of rifampicin into macrophages.
29600438	9	53	dep	RESULTS	1129:1135	arg1	increased					1145:1153	increased	1145:1153	increased	1145:1153	RESULTS The NPs increased the rate of uptake of rifampicin by a factor of 17 and 62 in case of PLGA and Glu-PLGA, respectively.
29600438	10	54	from	Expulsion	1257:1265	arg1	macrophages					1283:1293	the macrophages	1279:1293	the macrophages	1279:1293	Expulsion of NPs from the macrophages was also observed, which was 3 fold greater for Glu-PLGA NPs than for PLGA NPs.
29600438	6	55	theme	solvent	890:896	arg1	technique					910:918	an emulsion solvent evaporation technique	878:918	an emulsion solvent evaporation technique	878:918	Rifampicin loaded PLGA and Glu-PLGA NPs as well as rhodamine functionalized PLGA and Glu-PLGA NPs were synthesized using an emulsion solvent evaporation technique.
29600438	0	56	with	Functionalization	0:16	arg1	1,3-β-glucan					45:56	1,3-β-glucan	45:56	1,3-β-glucan	45:56	Functionalization of PLGA Nanoparticles with 1,3-β-glucan Enhances the Intracellular Pharmacokinetics of Rifampicin in Macrophages.
29600438	7	57	link	derived	998:1004	arg1	macrophages					1006:1016	THP-1 derived macrophages	992:1016	THP-1 derived macrophages	992:1016	Intracellular pharmacokinetics of rifampicin and NPs were evaluated in THP-1 derived macrophages.
29600438	9	58	theme	17	1203:1204	arg1	factor					1193:1198	a factor	1191:1198	a factor of 17 and 62	1191:1211	RESULTS The NPs increased the rate of uptake of rifampicin by a factor of 17 and 62 in case of PLGA and Glu-PLGA, respectively.
29600438	6	59	theme	emulsion	881:888	arg1	technique					910:918	an emulsion solvent evaporation technique	878:918	an emulsion solvent evaporation technique	878:918	Rifampicin loaded PLGA and Glu-PLGA NPs as well as rhodamine functionalized PLGA and Glu-PLGA NPs were synthesized using an emulsion solvent evaporation technique.
29600438	2	60	theme	loaded	294:299	arg1	NPs					316:318	NPs	316:318	NPs	316:318	1,3-β-glucan has been proposed as a ligand to target drug loaded nanoparticles (NPs) to macrophages.
29600438	2	60	theme	loaded	294:299	arg1	nanoparticles					301:313	drug loaded nanoparticles	289:313	drug loaded nanoparticles (NPs)	289:319	1,3-β-glucan has been proposed as a ligand to target drug loaded nanoparticles (NPs) to macrophages.
29600438	10	61	theme	PLGA	1365:1368	arg1	NPs					1370:1372	PLGA NPs	1365:1372	PLGA NPs	1365:1372	Expulsion of NPs from the macrophages was also observed, which was 3 fold greater for Glu-PLGA NPs than for PLGA NPs.
29600438	4	62	theme	rifampicin	631:640	arg1	amounts					620:626	higher amounts	613:626	higher amounts of rifampicin	613:640	We hypothesized that Glu-PLGA NPs would be taken up at a faster rate than PLGA NPs, and consequently deliver higher amounts of rifampicin into the macrophages.
29600438	4	62	theme	rifampicin	631:640	arg1	rifampicin					631:640	rifampicin	631:640	rifampicin	631:640	We hypothesized that Glu-PLGA NPs would be taken up at a faster rate than PLGA NPs, and consequently deliver higher amounts of rifampicin into the macrophages.
29600438	12	63	theme	rifampicin	1469:1478	arg1	rifampicin					1469:1478	rifampicin	1469:1478	rifampicin	1469:1478	After 24 h, the amount of rifampicin delivered by the PLGA and Glu-PLGA NPs was similar.
29600438	12	63	theme	rifampicin	1469:1478	arg1	amount					1459:1464	the amount	1455:1464	the amount of rifampicin delivered by the PLGA and Glu-PLGA NPs	1455:1517	After 24 h, the amount of rifampicin delivered by the PLGA and Glu-PLGA NPs was similar.
29600438	12	63	theme	rifampicin	1469:1478	arg1	similar					1523:1529	similar	1523:1529	similar	1523:1529	After 24 h, the amount of rifampicin delivered by the PLGA and Glu-PLGA NPs was similar.
29600438	6	64	theme	Glu-PLGA	784:791	arg1	NPs					793:795	Rifampicin loaded PLGA and Glu-PLGA NPs	757:795	NPs	793:795	Rifampicin loaded PLGA and Glu-PLGA NPs as well as rhodamine functionalized PLGA and Glu-PLGA NPs were synthesized using an emulsion solvent evaporation technique.
29600438	0	65	theme	Intracellular	71:83	arg1	Pharmacokinetics					85:100	the Intracellular Pharmacokinetics	67:100	the Intracellular Pharmacokinetics of Rifampicin in Macrophages	67:129	Functionalization of PLGA Nanoparticles with 1,3-β-glucan Enhances the Intracellular Pharmacokinetics of Rifampicin in Macrophages.
29600438	14	66	theme	PLGA	1642:1645	arg1	NPs					1647:1649	PLGA NPs	1642:1649	PLGA NPs	1642:1649	CONCLUSIONS Functionalization of PLGA NPs with 1,3-β-glucan resulted in faster uptake of rifampicin into macrophages.
29600438	0	67	theme	PLGA	21:24	arg1	Nanoparticles					26:38	PLGA Nanoparticles	21:38	PLGA Nanoparticles	21:38	Functionalization of PLGA Nanoparticles with 1,3-β-glucan Enhances the Intracellular Pharmacokinetics of Rifampicin in Macrophages.
29600438	7	68	dep	derived	998:1004	arg1	THP-1					992:996	THP-1	992:996	THP-1	992:996	Intracellular pharmacokinetics of rifampicin and NPs were evaluated in THP-1 derived macrophages.
30044900	10	0	theme	modified	1870:1877	arg1	pattern					1898:1904	a modified sugar accumulation pattern	1868:1904	a modified sugar accumulation pattern in the fleshy fruit	1868:1924	This article identifies the function of the tomato Fgr gene as a SWEET transporter, the upregulation of which leads to a modified sugar accumulation pattern in the fleshy fruit.
30044900	0	1	theme	tomato	162:167	arg1	fruit					169:173	ripening tomato fruit	153:173	ripening tomato fruit	153:173	Natural genetic variation for expression of a SWEET transporter among wild species of Solanum lycopersicum (tomato) determines the hexose composition of ripening tomato fruit.
30044900	2	2	theme	near-equimolar	325:338	arg1	levels					340:345	near-equimolar levels	325:345	near-equimolar levels of the hexoses glucose and fructose, derived from the hydrolysis of translocated sucrose	325:434	Cultivated tomato fruit are characterized by near-equimolar levels of the hexoses glucose and fructose, derived from the hydrolysis of translocated sucrose.
30044900	4	3	from	habrochaites	657:668	arg1	Introgressions					593:606	Introgressions	593:606	Introgressions of the FgrH allele from the wild species Solanum habrochaites (LA1777) into cultivated tomato	593:700	Introgressions of the FgrH allele from the wild species Solanum habrochaites (LA1777) into cultivated tomato increased the fructose-to-glucose ratio of the ripe fruit by reducing glucose levels and concomitantly increasing fructose levels.
30044900	9	4	theme	family	1645:1650	arg1	overexpression					1657:1670	the overexpression	1653:1670	the overexpression	1653:1670	These results indicate that the SlFgr gene encodes a plasma membrane-localized glucose efflux transporter of the SWEET family, the overexpression of which reduces glucose levels and may allow for increased fructose levels.
30044900	9	4	theme	family	1645:1650	arg1	transporter					1620:1630	a plasma membrane-localized glucose efflux transporter	1577:1630	a plasma membrane-localized glucose efflux transporter of the SWEET family, the overexpression of which reduces glucose levels and may allow for increased fructose levels	1577:1746	These results indicate that the SlFgr gene encodes a plasma membrane-localized glucose efflux transporter of the SWEET family, the overexpression of which reduces glucose levels and may allow for increased fructose levels.
30044900	9	4	theme	family	1645:1650	arg1	gene					1564:1567	the SlFgr gene	1554:1567	the SlFgr gene	1554:1567	These results indicate that the SlFgr gene encodes a plasma membrane-localized glucose efflux transporter of the SWEET family, the overexpression of which reduces glucose levels and may allow for increased fructose levels.
30044900	10	5	theme	fleshy	1913:1918	arg1	fruit					1920:1924	the fleshy fruit	1909:1924	the fleshy fruit	1909:1924	This article identifies the function of the tomato Fgr gene as a SWEET transporter, the upregulation of which leads to a modified sugar accumulation pattern in the fleshy fruit.
30044900	11	6	theme	wild	1978:1981	arg1	species					1983:1989	the inedible wild species	1965:1989	the inedible wild species	1965:1989	The results point to the potential of the inedible wild species to improve fruit sugar accumulation via sugar transport mechanisms.
30044900	1	7	theme	primary	238:244	arg1	content					186:192	The sugar content	176:192	The sugar content of Solanum lycopersicum (tomato) fruit	176:231	The sugar content of Solanum lycopersicum (tomato) fruit is a primary determinant of taste and quality.
30044900	1	7	theme	primary	238:244	arg1	determinant					246:256	a primary determinant	236:256	a primary determinant of taste and quality	236:277	The sugar content of Solanum lycopersicum (tomato) fruit is a primary determinant of taste and quality.
30044900	6	8	with	lines	1071:1075	arg1	ratio					1109:1113	a high fructose-to-glucose ratio	1082:1113	a high fructose-to-glucose ratio	1082:1113	The results indicated that a SWEET protein was strongly upregulated in the lines with a high fructose-to-glucose ratio.
30044900	4	9	theme	species	641:647	arg1	LA1777					671:676	LA1777	671:676	LA1777	671:676	Introgressions of the FgrH allele from the wild species Solanum habrochaites (LA1777) into cultivated tomato increased the fructose-to-glucose ratio of the ripe fruit by reducing glucose levels and concomitantly increasing fructose levels.
30044900	4	9	theme	species	641:647	arg1	habrochaites					657:668	the wild species Solanum habrochaites	632:668	the wild species Solanum habrochaites (LA1777) into cultivated tomato	632:700	Introgressions of the FgrH allele from the wild species Solanum habrochaites (LA1777) into cultivated tomato increased the fructose-to-glucose ratio of the ripe fruit by reducing glucose levels and concomitantly increasing fructose levels.
30044900	2	10	theme	Cultivated	280:289	arg1	fruit					298:302	Cultivated tomato fruit	280:302	Cultivated tomato fruit	280:302	Cultivated tomato fruit are characterized by near-equimolar levels of the hexoses glucose and fructose, derived from the hydrolysis of translocated sucrose.
30044900	11	11	theme	sugar	2008:2012	arg1	accumulation					2014:2025	fruit sugar accumulation	2002:2025	fruit sugar accumulation	2002:2025	The results point to the potential of the inedible wild species to improve fruit sugar accumulation via sugar transport mechanisms.
30044900	4	12	theme	allele	620:625	arg1	Introgressions					593:606	Introgressions	593:606	Introgressions of the FgrH allele from the wild species Solanum habrochaites (LA1777) into cultivated tomato	593:700	Introgressions of the FgrH allele from the wild species Solanum habrochaites (LA1777) into cultivated tomato increased the fructose-to-glucose ratio of the ripe fruit by reducing glucose levels and concomitantly increasing fructose levels.
30044900	3	13	theme	tomato	573:578	arg1	taste					586:590	tomato fruit taste	573:590	tomato fruit taste	573:590	As fructose is perceived as approximately twice as sweet as glucose, increasing its concentration at the expense of glucose can improve tomato fruit taste.
30044900	1	14	theme	Solanum	197:203	arg1	fruit					227:231	Solanum lycopersicum (tomato) fruit	197:231	Solanum lycopersicum (tomato) fruit	197:231	The sugar content of Solanum lycopersicum (tomato) fruit is a primary determinant of taste and quality.
30044900	0	15	theme	hexose	131:136	arg1	composition					138:148	the hexose composition	127:148	the hexose composition of ripening tomato fruit	127:173	Natural genetic variation for expression of a SWEET transporter among wild species of Solanum lycopersicum (tomato) determines the hexose composition of ripening tomato fruit.
30044900	11	16	theme	transport	2037:2045	arg1	mechanisms					2047:2056	sugar transport mechanisms	2031:2056	sugar transport mechanisms	2031:2056	The results point to the potential of the inedible wild species to improve fruit sugar accumulation via sugar transport mechanisms.
30044900	8	17	theme	native	1442:1447	arg1	transporters					1456:1467	native hexose transporters	1442:1467	native hexose transporters	1442:1467	The SWEET protein was localized to the plasma membrane and expression of the SlFgr gene in a yeast line lacking native hexose transporters complemented growth with glucose, but not with fructose.
30044900	9	18	theme	fructose	1732:1739	arg1	levels					1741:1746	increased fructose levels	1722:1746	increased fructose levels	1722:1746	These results indicate that the SlFgr gene encodes a plasma membrane-localized glucose efflux transporter of the SWEET family, the overexpression of which reduces glucose levels and may allow for increased fructose levels.
30044900	7	19	theme	fructose 	1235:1243	arg1	ratio					1254:1258	the fructose : glucose ratio	1231:1258	the fructose : glucose ratio in the developing fruit	1231:1282	Overexpressing the SWEET protein in transgenic tomato plants dramatically reduced the glucose levels and increased the fructose : glucose ratio in the developing fruit, thereby proving the function of the protein.
30044900	10	20	theme	gene	1804:1807	arg1	transporter					1820:1830	a SWEET transporter	1812:1830	a SWEET transporter	1812:1830	This article identifies the function of the tomato Fgr gene as a SWEET transporter, the upregulation of which leads to a modified sugar accumulation pattern in the fleshy fruit.
30044900	10	20	theme	gene	1804:1807	arg1	function					1777:1784	the function	1773:1784	the function of the tomato Fgr gene	1773:1807	This article identifies the function of the tomato Fgr gene as a SWEET transporter, the upregulation of which leads to a modified sugar accumulation pattern in the fleshy fruit.
30044900	0	21	theme	wild	70:73	arg1	species					75:81	wild species	70:81	wild species of Solanum lycopersicum (tomato)	70:114	Natural genetic variation for expression of a SWEET transporter among wild species of Solanum lycopersicum (tomato) determines the hexose composition of ripening tomato fruit.
30044900	5	22	theme	gene	878:881	arg1	function					858:865	the function	854:865	the function of the Fgr gene	854:881	In order to identify the function of the Fgr gene, we combined a fine-mapping strategy with RNAseq differential expression analysis of near-isogenic tomato lines.
30044900	8	23	theme	SWEET	1334:1338	arg1	protein					1340:1346	The SWEET protein	1330:1346	The SWEET protein	1330:1346	The SWEET protein was localized to the plasma membrane and expression of the SlFgr gene in a yeast line lacking native hexose transporters complemented growth with glucose, but not with fructose.
30044900	5	24	theme	expression	945:954	arg1	analysis					956:963	RNAseq differential expression analysis	925:963	RNAseq differential expression analysis of near-isogenic tomato lines	925:993	In order to identify the function of the Fgr gene, we combined a fine-mapping strategy with RNAseq differential expression analysis of near-isogenic tomato lines.
30044900	4	25	theme	fruit	754:758	arg1	ratio					736:740	the fructose-to-glucose ratio	712:740	the fructose-to-glucose ratio of the ripe fruit	712:758	Introgressions of the FgrH allele from the wild species Solanum habrochaites (LA1777) into cultivated tomato increased the fructose-to-glucose ratio of the ripe fruit by reducing glucose levels and concomitantly increasing fructose levels.
30044900	0	26	theme	lycopersicum	94:105	arg1	species					75:81	wild species	70:81	wild species of Solanum lycopersicum (tomato)	70:114	Natural genetic variation for expression of a SWEET transporter among wild species of Solanum lycopersicum (tomato) determines the hexose composition of ripening tomato fruit.
30044900	2	27	theme	sucrose	428:434	arg1	hydrolysis					401:410	the hydrolysis	397:410	the hydrolysis of translocated sucrose	397:434	Cultivated tomato fruit are characterized by near-equimolar levels of the hexoses glucose and fructose, derived from the hydrolysis of translocated sucrose.
30044900	0	28	theme	Natural	0:6	arg1	variation					16:24	Natural genetic variation	0:24	Natural genetic variation for expression of a SWEET transporter among wild species of Solanum lycopersicum (tomato)	0:114	Natural genetic variation for expression of a SWEET transporter among wild species of Solanum lycopersicum (tomato) determines the hexose composition of ripening tomato fruit.
30044900	8	29	theme	plasma	1369:1374	arg1	membrane					1376:1383	the plasma membrane	1365:1383	the plasma membrane	1365:1383	The SWEET protein was localized to the plasma membrane and expression of the SlFgr gene in a yeast line lacking native hexose transporters complemented growth with glucose, but not with fructose.
30044900	7	30	theme	tomato	1163:1168	arg1	plants					1170:1175	transgenic tomato plants	1152:1175	transgenic tomato plants	1152:1175	Overexpressing the SWEET protein in transgenic tomato plants dramatically reduced the glucose levels and increased the fructose : glucose ratio in the developing fruit, thereby proving the function of the protein.
30044900	1	31	theme	taste	261:265	arg1	content					186:192	The sugar content	176:192	The sugar content of Solanum lycopersicum (tomato) fruit	176:231	The sugar content of Solanum lycopersicum (tomato) fruit is a primary determinant of taste and quality.
30044900	1	31	theme	taste	261:265	arg1	determinant					246:256	a primary determinant	236:256	a primary determinant of taste and quality	236:277	The sugar content of Solanum lycopersicum (tomato) fruit is a primary determinant of taste and quality.
30044900	5	32	theme	RNAseq	925:930	arg1	analysis					956:963	RNAseq differential expression analysis	925:963	RNAseq differential expression analysis of near-isogenic tomato lines	925:993	In order to identify the function of the Fgr gene, we combined a fine-mapping strategy with RNAseq differential expression analysis of near-isogenic tomato lines.
30044900	0	33	theme	transporter	52:62	arg1	expression					30:39	expression	30:39	expression of a SWEET transporter	30:62	Natural genetic variation for expression of a SWEET transporter among wild species of Solanum lycopersicum (tomato) determines the hexose composition of ripening tomato fruit.
30044900	1	34	theme	quality	271:277	arg1	content					186:192	The sugar content	176:192	The sugar content of Solanum lycopersicum (tomato) fruit	176:231	The sugar content of Solanum lycopersicum (tomato) fruit is a primary determinant of taste and quality.
30044900	1	34	theme	quality	271:277	arg1	determinant					246:256	a primary determinant	236:256	a primary determinant of taste and quality	236:277	The sugar content of Solanum lycopersicum (tomato) fruit is a primary determinant of taste and quality.
30044900	10	35	theme	transporter	1820:1830	arg1	upregulation					1837:1848	the upregulation	1833:1848	the upregulation	1833:1848	This article identifies the function of the tomato Fgr gene as a SWEET transporter, the upregulation of which leads to a modified sugar accumulation pattern in the fleshy fruit.
30044900	4	36	theme	fructose	816:823	arg1	levels					825:830	fructose levels	816:830	fructose levels	816:830	Introgressions of the FgrH allele from the wild species Solanum habrochaites (LA1777) into cultivated tomato increased the fructose-to-glucose ratio of the ripe fruit by reducing glucose levels and concomitantly increasing fructose levels.
30044900	7	37	theme	SWEET	1135:1139	arg1	protein					1141:1147	the SWEET protein	1131:1147	the SWEET protein	1131:1147	Overexpressing the SWEET protein in transgenic tomato plants dramatically reduced the glucose levels and increased the fructose : glucose ratio in the developing fruit, thereby proving the function of the protein.
30044900	9	38	theme	membrane-localized	1586:1603	arg1	transporter					1620:1630	a plasma membrane-localized glucose efflux transporter	1577:1630	a plasma membrane-localized glucose efflux transporter of the SWEET family, the overexpression of which reduces glucose levels and may allow for increased fructose levels	1577:1746	These results indicate that the SlFgr gene encodes a plasma membrane-localized glucose efflux transporter of the SWEET family, the overexpression of which reduces glucose levels and may allow for increased fructose levels.
30044900	9	38	theme	membrane-localized	1586:1603	arg1	gene					1564:1567	the SlFgr gene	1554:1567	the SlFgr gene	1554:1567	These results indicate that the SlFgr gene encodes a plasma membrane-localized glucose efflux transporter of the SWEET family, the overexpression of which reduces glucose levels and may allow for increased fructose levels.
30044900	8	39	theme	SlFgr	1407:1411	arg1	gene					1413:1416	the SlFgr gene	1403:1416	the SlFgr gene	1403:1416	The SWEET protein was localized to the plasma membrane and expression of the SlFgr gene in a yeast line lacking native hexose transporters complemented growth with glucose, but not with fructose.
30044900	1	40	theme	lycopersicum	205:216	arg1	fruit					227:231	Solanum lycopersicum (tomato) fruit	197:231	Solanum lycopersicum (tomato) fruit	197:231	The sugar content of Solanum lycopersicum (tomato) fruit is a primary determinant of taste and quality.
30044900	5	41	theme	tomato	982:987	arg1	lines					989:993	near-isogenic tomato lines	968:993	near-isogenic tomato lines	968:993	In order to identify the function of the Fgr gene, we combined a fine-mapping strategy with RNAseq differential expression analysis of near-isogenic tomato lines.
30044900	7	42	theme	developing	1267:1276	arg1	fruit					1278:1282	the developing fruit	1263:1282	the developing fruit	1263:1282	Overexpressing the SWEET protein in transgenic tomato plants dramatically reduced the glucose levels and increased the fructose : glucose ratio in the developing fruit, thereby proving the function of the protein.
30044900	1	43	theme	tomato	219:224	arg1	fruit					227:231	Solanum lycopersicum (tomato) fruit	197:231	Solanum lycopersicum (tomato) fruit	197:231	The sugar content of Solanum lycopersicum (tomato) fruit is a primary determinant of taste and quality.
30044900	10	44	theme	sugar	1879:1883	arg1	pattern					1898:1904	a modified sugar accumulation pattern	1868:1904	a modified sugar accumulation pattern in the fleshy fruit	1868:1924	This article identifies the function of the tomato Fgr gene as a SWEET transporter, the upregulation of which leads to a modified sugar accumulation pattern in the fleshy fruit.
30044900	0	45	theme	fruit	169:173	arg1	composition					138:148	the hexose composition	127:148	the hexose composition of ripening tomato fruit	127:173	Natural genetic variation for expression of a SWEET transporter among wild species of Solanum lycopersicum (tomato) determines the hexose composition of ripening tomato fruit.
30044900	1	46	theme	fruit	227:231	arg1	content					186:192	The sugar content	176:192	The sugar content of Solanum lycopersicum (tomato) fruit	176:231	The sugar content of Solanum lycopersicum (tomato) fruit is a primary determinant of taste and quality.
30044900	1	46	theme	fruit	227:231	arg1	determinant					246:256	a primary determinant	236:256	a primary determinant of taste and quality	236:277	The sugar content of Solanum lycopersicum (tomato) fruit is a primary determinant of taste and quality.
30044900	9	47	theme	SWEET	1639:1643	arg1	family					1645:1650	the SWEET family	1635:1650	the SWEET family	1635:1650	These results indicate that the SlFgr gene encodes a plasma membrane-localized glucose efflux transporter of the SWEET family, the overexpression of which reduces glucose levels and may allow for increased fructose levels.
30044900	7	48	theme	 glucose	1245:1252	arg1	ratio					1254:1258	the fructose : glucose ratio	1231:1258	the fructose : glucose ratio in the developing fruit	1231:1282	Overexpressing the SWEET protein in transgenic tomato plants dramatically reduced the glucose levels and increased the fructose : glucose ratio in the developing fruit, thereby proving the function of the protein.
30044900	11	49	theme	inedible	1969:1976	arg1	species					1983:1989	the inedible wild species	1965:1989	the inedible wild species	1965:1989	The results point to the potential of the inedible wild species to improve fruit sugar accumulation via sugar transport mechanisms.
30044900	7	50	from	ratio	1254:1258	arg1	fruit					1278:1282	the developing fruit	1263:1282	the developing fruit	1263:1282	Overexpressing the SWEET protein in transgenic tomato plants dramatically reduced the glucose levels and increased the fructose : glucose ratio in the developing fruit, thereby proving the function of the protein.
30044900	8	51	from	expression	1389:1398	arg1	line					1429:1432	a yeast line	1421:1432	a yeast line lacking native hexose transporters	1421:1467	The SWEET protein was localized to the plasma membrane and expression of the SlFgr gene in a yeast line lacking native hexose transporters complemented growth with glucose, but not with fructose.
30044900	2	52	theme	tomato	291:296	arg1	fruit					298:302	Cultivated tomato fruit	280:302	Cultivated tomato fruit	280:302	Cultivated tomato fruit are characterized by near-equimolar levels of the hexoses glucose and fructose, derived from the hydrolysis of translocated sucrose.
30044900	4	53	theme	wild	636:639	arg1	LA1777					671:676	LA1777	671:676	LA1777	671:676	Introgressions of the FgrH allele from the wild species Solanum habrochaites (LA1777) into cultivated tomato increased the fructose-to-glucose ratio of the ripe fruit by reducing glucose levels and concomitantly increasing fructose levels.
30044900	4	53	theme	wild	636:639	arg1	habrochaites					657:668	the wild species Solanum habrochaites	632:668	the wild species Solanum habrochaites (LA1777) into cultivated tomato	632:700	Introgressions of the FgrH allele from the wild species Solanum habrochaites (LA1777) into cultivated tomato increased the fructose-to-glucose ratio of the ripe fruit by reducing glucose levels and concomitantly increasing fructose levels.
30044900	11	54	theme	species	1983:1989	arg1	potential					1952:1960	the potential	1948:1960	the potential of the inedible wild species to improve fruit sugar accumulation via sugar transport mechanisms	1948:2056	The results point to the potential of the inedible wild species to improve fruit sugar accumulation via sugar transport mechanisms.
30044900	1	55	theme	sugar	180:184	arg1	content					186:192	The sugar content	176:192	The sugar content of Solanum lycopersicum (tomato) fruit	176:231	The sugar content of Solanum lycopersicum (tomato) fruit is a primary determinant of taste and quality.
30044900	1	55	theme	sugar	180:184	arg1	determinant					246:256	a primary determinant	236:256	a primary determinant of taste and quality	236:277	The sugar content of Solanum lycopersicum (tomato) fruit is a primary determinant of taste and quality.
30044900	11	56	theme	fruit	2002:2006	arg1	accumulation					2014:2025	fruit sugar accumulation	2002:2025	fruit sugar accumulation	2002:2025	The results point to the potential of the inedible wild species to improve fruit sugar accumulation via sugar transport mechanisms.
30044900	9	57	theme	glucose	1689:1695	arg1	levels					1697:1702	glucose levels	1689:1702	glucose levels	1689:1702	These results indicate that the SlFgr gene encodes a plasma membrane-localized glucose efflux transporter of the SWEET family, the overexpression of which reduces glucose levels and may allow for increased fructose levels.
30044900	8	58	theme	yeast	1423:1427	arg1	line					1429:1432	a yeast line	1421:1432	a yeast line lacking native hexose transporters	1421:1467	The SWEET protein was localized to the plasma membrane and expression of the SlFgr gene in a yeast line lacking native hexose transporters complemented growth with glucose, but not with fructose.
30044900	10	59	theme	Fgr	1800:1802	arg1	gene					1804:1807	the tomato Fgr gene	1789:1807	the tomato Fgr gene	1789:1807	This article identifies the function of the tomato Fgr gene as a SWEET transporter, the upregulation of which leads to a modified sugar accumulation pattern in the fleshy fruit.
30044900	10	60	from	pattern	1898:1904	arg1	fruit					1920:1924	the fleshy fruit	1909:1924	the fleshy fruit	1909:1924	This article identifies the function of the tomato Fgr gene as a SWEET transporter, the upregulation of which leads to a modified sugar accumulation pattern in the fleshy fruit.
30044900	4	61	theme	FgrH	615:618	arg1	allele					620:625	the FgrH allele	611:625	the FgrH allele	611:625	Introgressions of the FgrH allele from the wild species Solanum habrochaites (LA1777) into cultivated tomato increased the fructose-to-glucose ratio of the ripe fruit by reducing glucose levels and concomitantly increasing fructose levels.
30044900	3	62	theme	glucose	553:559	arg1	expense					542:548	the expense	538:548	the expense of glucose	538:559	As fructose is perceived as approximately twice as sweet as glucose, increasing its concentration at the expense of glucose can improve tomato fruit taste.
30044900	8	63	theme	hexose	1449:1454	arg1	transporters					1456:1467	native hexose transporters	1442:1467	native hexose transporters	1442:1467	The SWEET protein was localized to the plasma membrane and expression of the SlFgr gene in a yeast line lacking native hexose transporters complemented growth with glucose, but not with fructose.
30044900	0	64	theme	ripening	153:160	arg1	fruit					169:173	ripening tomato fruit	153:173	ripening tomato fruit	153:173	Natural genetic variation for expression of a SWEET transporter among wild species of Solanum lycopersicum (tomato) determines the hexose composition of ripening tomato fruit.
30044900	9	65	theme	glucose	1605:1611	arg1	transporter					1620:1630	a plasma membrane-localized glucose efflux transporter	1577:1630	a plasma membrane-localized glucose efflux transporter of the SWEET family, the overexpression of which reduces glucose levels and may allow for increased fructose levels	1577:1746	These results indicate that the SlFgr gene encodes a plasma membrane-localized glucose efflux transporter of the SWEET family, the overexpression of which reduces glucose levels and may allow for increased fructose levels.
30044900	9	65	theme	glucose	1605:1611	arg1	gene					1564:1567	the SlFgr gene	1554:1567	the SlFgr gene	1554:1567	These results indicate that the SlFgr gene encodes a plasma membrane-localized glucose efflux transporter of the SWEET family, the overexpression of which reduces glucose levels and may allow for increased fructose levels.
30044900	10	66	theme	SWEET	1814:1818	arg1	transporter					1820:1830	a SWEET transporter	1812:1830	a SWEET transporter	1812:1830	This article identifies the function of the tomato Fgr gene as a SWEET transporter, the upregulation of which leads to a modified sugar accumulation pattern in the fleshy fruit.
30044900	10	66	theme	SWEET	1814:1818	arg1	function					1777:1784	the function	1773:1784	the function of the tomato Fgr gene	1773:1807	This article identifies the function of the tomato Fgr gene as a SWEET transporter, the upregulation of which leads to a modified sugar accumulation pattern in the fleshy fruit.
30044900	9	67	theme	increased	1722:1730	arg1	levels					1741:1746	increased fructose levels	1722:1746	increased fructose levels	1722:1746	These results indicate that the SlFgr gene encodes a plasma membrane-localized glucose efflux transporter of the SWEET family, the overexpression of which reduces glucose levels and may allow for increased fructose levels.
30044900	4	68	theme	cultivated	684:693	arg1	tomato					695:700	cultivated tomato	684:700	cultivated tomato	684:700	Introgressions of the FgrH allele from the wild species Solanum habrochaites (LA1777) into cultivated tomato increased the fructose-to-glucose ratio of the ripe fruit by reducing glucose levels and concomitantly increasing fructose levels.
30044900	3	69	theme	fruit	580:584	arg1	taste					586:590	tomato fruit taste	573:590	tomato fruit taste	573:590	As fructose is perceived as approximately twice as sweet as glucose, increasing its concentration at the expense of glucose can improve tomato fruit taste.
30044900	4	70	theme	Solanum	649:655	arg1	LA1777					671:676	LA1777	671:676	LA1777	671:676	Introgressions of the FgrH allele from the wild species Solanum habrochaites (LA1777) into cultivated tomato increased the fructose-to-glucose ratio of the ripe fruit by reducing glucose levels and concomitantly increasing fructose levels.
30044900	4	70	theme	Solanum	649:655	arg1	habrochaites					657:668	the wild species Solanum habrochaites	632:668	the wild species Solanum habrochaites (LA1777) into cultivated tomato	632:700	Introgressions of the FgrH allele from the wild species Solanum habrochaites (LA1777) into cultivated tomato increased the fructose-to-glucose ratio of the ripe fruit by reducing glucose levels and concomitantly increasing fructose levels.
30044900	5	71	theme	Fgr	874:876	arg1	gene					878:881	the Fgr gene	870:881	the Fgr gene	870:881	In order to identify the function of the Fgr gene, we combined a fine-mapping strategy with RNAseq differential expression analysis of near-isogenic tomato lines.
30044900	11	72	theme	sugar	2031:2035	arg1	mechanisms					2047:2056	sugar transport mechanisms	2031:2056	sugar transport mechanisms	2031:2056	The results point to the potential of the inedible wild species to improve fruit sugar accumulation via sugar transport mechanisms.
30044900	7	73	theme	glucose	1202:1208	arg1	levels					1210:1215	the glucose levels	1198:1215	the glucose levels	1198:1215	Overexpressing the SWEET protein in transgenic tomato plants dramatically reduced the glucose levels and increased the fructose : glucose ratio in the developing fruit, thereby proving the function of the protein.
30044900	9	74	theme	efflux	1613:1618	arg1	transporter					1620:1630	a plasma membrane-localized glucose efflux transporter	1577:1630	a plasma membrane-localized glucose efflux transporter of the SWEET family, the overexpression of which reduces glucose levels and may allow for increased fructose levels	1577:1746	These results indicate that the SlFgr gene encodes a plasma membrane-localized glucose efflux transporter of the SWEET family, the overexpression of which reduces glucose levels and may allow for increased fructose levels.
30044900	9	74	theme	efflux	1613:1618	arg1	gene					1564:1567	the SlFgr gene	1554:1567	the SlFgr gene	1554:1567	These results indicate that the SlFgr gene encodes a plasma membrane-localized glucose efflux transporter of the SWEET family, the overexpression of which reduces glucose levels and may allow for increased fructose levels.
30044900	5	75	theme	differential	932:943	arg1	analysis					956:963	RNAseq differential expression analysis	925:963	RNAseq differential expression analysis of near-isogenic tomato lines	925:993	In order to identify the function of the Fgr gene, we combined a fine-mapping strategy with RNAseq differential expression analysis of near-isogenic tomato lines.
30044900	6	76	theme	fructose-to-glucose	1089:1107	arg1	ratio					1109:1113	a high fructose-to-glucose ratio	1082:1113	a high fructose-to-glucose ratio	1082:1113	The results indicated that a SWEET protein was strongly upregulated in the lines with a high fructose-to-glucose ratio.
30044900	7	77	theme	protein	1321:1327	arg1	function					1305:1312	the function	1301:1312	the function of the protein	1301:1327	Overexpressing the SWEET protein in transgenic tomato plants dramatically reduced the glucose levels and increased the fructose : glucose ratio in the developing fruit, thereby proving the function of the protein.
30044900	4	78	theme	ripe	749:752	arg1	fruit					754:758	the ripe fruit	745:758	the ripe fruit	745:758	Introgressions of the FgrH allele from the wild species Solanum habrochaites (LA1777) into cultivated tomato increased the fructose-to-glucose ratio of the ripe fruit by reducing glucose levels and concomitantly increasing fructose levels.
30044900	0	79	theme	genetic	8:14	arg1	variation					16:24	Natural genetic variation	0:24	Natural genetic variation for expression of a SWEET transporter among wild species of Solanum lycopersicum (tomato)	0:114	Natural genetic variation for expression of a SWEET transporter among wild species of Solanum lycopersicum (tomato) determines the hexose composition of ripening tomato fruit.
30044900	5	80	theme	fine-mapping	898:909	arg1	strategy					911:918	a fine-mapping strategy	896:918	a fine-mapping strategy	896:918	In order to identify the function of the Fgr gene, we combined a fine-mapping strategy with RNAseq differential expression analysis of near-isogenic tomato lines.
30044900	2	81	theme	translocated	415:426	arg1	sucrose					428:434	translocated sucrose	415:434	translocated sucrose	415:434	Cultivated tomato fruit are characterized by near-equimolar levels of the hexoses glucose and fructose, derived from the hydrolysis of translocated sucrose.
30044900	2	82	theme	hexoses	354:360	arg1	levels					340:345	near-equimolar levels	325:345	near-equimolar levels of the hexoses glucose and fructose, derived from the hydrolysis of translocated sucrose	325:434	Cultivated tomato fruit are characterized by near-equimolar levels of the hexoses glucose and fructose, derived from the hydrolysis of translocated sucrose.
30044900	7	83	theme	transgenic	1152:1161	arg1	plants					1170:1175	transgenic tomato plants	1152:1175	transgenic tomato plants	1152:1175	Overexpressing the SWEET protein in transgenic tomato plants dramatically reduced the glucose levels and increased the fructose : glucose ratio in the developing fruit, thereby proving the function of the protein.
30044900	9	84	theme	SlFgr	1558:1562	arg1	transporter					1620:1630	a plasma membrane-localized glucose efflux transporter	1577:1630	a plasma membrane-localized glucose efflux transporter of the SWEET family, the overexpression of which reduces glucose levels and may allow for increased fructose levels	1577:1746	These results indicate that the SlFgr gene encodes a plasma membrane-localized glucose efflux transporter of the SWEET family, the overexpression of which reduces glucose levels and may allow for increased fructose levels.
30044900	9	84	theme	SlFgr	1558:1562	arg1	gene					1564:1567	the SlFgr gene	1554:1567	the SlFgr gene	1554:1567	These results indicate that the SlFgr gene encodes a plasma membrane-localized glucose efflux transporter of the SWEET family, the overexpression of which reduces glucose levels and may allow for increased fructose levels.
30044900	4	85	theme	fructose-to-glucose	716:734	arg1	ratio					736:740	the fructose-to-glucose ratio	712:740	the fructose-to-glucose ratio of the ripe fruit	712:758	Introgressions of the FgrH allele from the wild species Solanum habrochaites (LA1777) into cultivated tomato increased the fructose-to-glucose ratio of the ripe fruit by reducing glucose levels and concomitantly increasing fructose levels.
30044900	6	86	theme	SWEET	1025:1029	arg1	protein					1031:1037	a SWEET protein	1023:1037	a SWEET protein	1023:1037	The results indicated that a SWEET protein was strongly upregulated in the lines with a high fructose-to-glucose ratio.
30044900	0	87	theme	SWEET	46:50	arg1	transporter					52:62	a SWEET transporter	44:62	a SWEET transporter	44:62	Natural genetic variation for expression of a SWEET transporter among wild species of Solanum lycopersicum (tomato) determines the hexose composition of ripening tomato fruit.
30044900	2	88	attach	derived	384:390	arg2	hexoses					354:360	the hexoses glucose and fructose	350:381	the hexoses glucose and fructose	350:381	Cultivated tomato fruit are characterized by near-equimolar levels of the hexoses glucose and fructose, derived from the hydrolysis of translocated sucrose.
30044900	2	88	attach	derived	384:390	arg1	hydrolysis					401:410	the hydrolysis	397:410	the hydrolysis of translocated sucrose	397:434	Cultivated tomato fruit are characterized by near-equimolar levels of the hexoses glucose and fructose, derived from the hydrolysis of translocated sucrose.
30044900	2	88	attach	derived	384:390	arg2	fructose					374:381	fructose	374:381	fructose	374:381	Cultivated tomato fruit are characterized by near-equimolar levels of the hexoses glucose and fructose, derived from the hydrolysis of translocated sucrose.
30044900	2	88	attach	derived	384:390	arg2	glucose					362:368	glucose	362:368	glucose	362:368	Cultivated tomato fruit are characterized by near-equimolar levels of the hexoses glucose and fructose, derived from the hydrolysis of translocated sucrose.
30044900	4	89	theme	glucose	772:778	arg1	levels					780:785	glucose levels	772:785	glucose levels	772:785	Introgressions of the FgrH allele from the wild species Solanum habrochaites (LA1777) into cultivated tomato increased the fructose-to-glucose ratio of the ripe fruit by reducing glucose levels and concomitantly increasing fructose levels.
30044900	5	90	theme	near-isogenic	968:980	arg1	lines					989:993	near-isogenic tomato lines	968:993	near-isogenic tomato lines	968:993	In order to identify the function of the Fgr gene, we combined a fine-mapping strategy with RNAseq differential expression analysis of near-isogenic tomato lines.
30044900	10	91	theme	tomato	1793:1798	arg1	gene					1804:1807	the tomato Fgr gene	1789:1807	the tomato Fgr gene	1789:1807	This article identifies the function of the tomato Fgr gene as a SWEET transporter, the upregulation of which leads to a modified sugar accumulation pattern in the fleshy fruit.
30044900	2	92	dep	hexoses	354:360	arg1	hexoses					354:360	the hexoses glucose and fructose	350:381	the hexoses glucose and fructose	350:381	Cultivated tomato fruit are characterized by near-equimolar levels of the hexoses glucose and fructose, derived from the hydrolysis of translocated sucrose.
30044900	2	92	dep	hexoses	354:360	arg1	fructose					374:381	fructose	374:381	fructose	374:381	Cultivated tomato fruit are characterized by near-equimolar levels of the hexoses glucose and fructose, derived from the hydrolysis of translocated sucrose.
30044900	2	92	dep	hexoses	354:360	arg1	glucose					362:368	glucose	362:368	glucose	362:368	Cultivated tomato fruit are characterized by near-equimolar levels of the hexoses glucose and fructose, derived from the hydrolysis of translocated sucrose.
30044900	5	93	theme	lines	989:993	arg1	analysis					956:963	RNAseq differential expression analysis	925:963	RNAseq differential expression analysis of near-isogenic tomato lines	925:993	In order to identify the function of the Fgr gene, we combined a fine-mapping strategy with RNAseq differential expression analysis of near-isogenic tomato lines.
30044900	6	94	theme	high	1084:1087	arg1	ratio					1109:1113	a high fructose-to-glucose ratio	1082:1113	a high fructose-to-glucose ratio	1082:1113	The results indicated that a SWEET protein was strongly upregulated in the lines with a high fructose-to-glucose ratio.
30044900	10	95	theme	accumulation	1885:1896	arg1	pattern					1898:1904	a modified sugar accumulation pattern	1868:1904	a modified sugar accumulation pattern in the fleshy fruit	1868:1924	This article identifies the function of the tomato Fgr gene as a SWEET transporter, the upregulation of which leads to a modified sugar accumulation pattern in the fleshy fruit.
30044900	8	96	theme	gene	1413:1416	arg1	expression					1389:1398	expression	1389:1398	expression of the SlFgr gene in a yeast line lacking native hexose transporters	1389:1467	The SWEET protein was localized to the plasma membrane and expression of the SlFgr gene in a yeast line lacking native hexose transporters complemented growth with glucose, but not with fructose.
31424384	11	0	dep	anteiso-C15 	1209:1220	arg1	iso-C16 					1246:1253	iso-C16 	1246:1253	iso-C16 	1246:1253	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
31424384	11	0	dep	anteiso-C15 	1209:1220	arg1	 0					1239:1240	 0	1239:1240	 0	1239:1240	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
31424384	11	0	dep	anteiso-C15 	1209:1220	arg1	 0					1222:1223	 0	1222:1223	 0	1222:1223	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
31424384	11	0	dep	anteiso-C15 	1209:1220	arg1	 0					1255:1256	 0	1255:1256	 0	1255:1256	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
31424384	5	1	theme	16S	587:589	arg1	similarity					610:619	16S rRNA gene sequence similarity	587:619	16S rRNA gene sequence similarity	587:619	Based on 16S rRNA gene sequence similarity, strain SSKP1-9T represents a distinct novel species, as shown by phenotypic traits, DNA-DNA hybridization and average nucleotide identity values.
31424384	10	2	theme	phosphatidylglycerol	1040:1059	arg1	presence					1028:1035	the presence	1024:1035	the presence of phosphatidylglycerol, diphosphatidylglycerol, four unidentified lipids, an unidentified phospholipid and an unidentified glycolipid	1024:1170	Polar lipid analysis revealed the presence of phosphatidylglycerol, diphosphatidylglycerol, four unidentified lipids, an unidentified phospholipid and an unidentified glycolipid.
31424384	12	3	theme	Lentibacillus	1405:1417	arg1	species					1394:1400	a novel species	1386:1400	a novel species	1386:1400	The results of phenotypic and chemotaxonomic characteristics and whole-genome analysis support that strain SSKP1-9T represents a novel species of Lentibacillus, for which the name Lentibacilluslipolyticus sp.
31424384	4	4	dep	Lentibacillus	461:473	arg1	juripiscarius					475:487	juripiscarius	475:487	juripiscarius	475:487	The 16S rRNA gene sequence similarity values between strain SSKP1-9T and Lentibacillus juripiscarius TISTR 1535T and Lentibacillus halophilus TISTR 1549T were 98.7 and 97.2 %, respectively.
31424384	5	5	theme	rRNA	591:594	arg1	similarity					610:619	16S rRNA gene sequence similarity	587:619	16S rRNA gene sequence similarity	587:619	Based on 16S rRNA gene sequence similarity, strain SSKP1-9T represents a distinct novel species, as shown by phenotypic traits, DNA-DNA hybridization and average nucleotide identity values.
31424384	8	6	theme	isoprenoid	901:910	arg1	quinone					912:918	The major isoprenoid quinone	891:918	The major isoprenoid quinone	891:918	The major isoprenoid quinone was MK-7.
31424384	8	6	theme	isoprenoid	901:910	arg1	MK-7					924:927	MK-7	924:927	MK-7	924:927	The major isoprenoid quinone was MK-7.
31424384	4	7	theme	TISTR	530:534	arg1	1549T					536:540	Lentibacillus halophilus TISTR 1549T	505:540	Lentibacillus halophilus TISTR 1549T	505:540	The 16S rRNA gene sequence similarity values between strain SSKP1-9T and Lentibacillus juripiscarius TISTR 1535T and Lentibacillus halophilus TISTR 1549T were 98.7 and 97.2 %, respectively.
31424384	4	8	theme	strain	441:446	arg1	SSKP1-9T					448:455	strain SSKP1-9T	441:455	strain SSKP1-9T	441:455	The 16S rRNA gene sequence similarity values between strain SSKP1-9T and Lentibacillus juripiscarius TISTR 1535T and Lentibacillus halophilus TISTR 1549T were 98.7 and 97.2 %, respectively.
31424384	5	9	theme	gene	596:599	arg1	similarity					610:619	16S rRNA gene sequence similarity	587:619	16S rRNA gene sequence similarity	587:619	Based on 16S rRNA gene sequence similarity, strain SSKP1-9T represents a distinct novel species, as shown by phenotypic traits, DNA-DNA hybridization and average nucleotide identity values.
31424384	8	10	theme	major	895:899	arg1	quinone					912:918	The major isoprenoid quinone	891:918	The major isoprenoid quinone	891:918	The major isoprenoid quinone was MK-7.
31424384	8	10	theme	major	895:899	arg1	MK-7					924:927	MK-7	924:927	MK-7	924:927	The major isoprenoid quinone was MK-7.
31424384	1	11	theme	halophilic	49:58	arg1	bacterium					60:68	a moderately halophilic bacterium	36:68	a moderately halophilic bacterium isolated from shrimp paste (Ka-pi)	36:103	nov., a moderately halophilic bacterium isolated from shrimp paste (Ka-pi).
31424384	10	12	theme	unidentified	1148:1159	arg1	glycolipid					1161:1170	an unidentified glycolipid	1145:1170	an unidentified glycolipid	1145:1170	Polar lipid analysis revealed the presence of phosphatidylglycerol, diphosphatidylglycerol, four unidentified lipids, an unidentified phospholipid and an unidentified glycolipid.
31424384	2	13	attach	isolated	200:207	arg1	paste					240:244	traditional salted shrimp paste	214:244	traditional salted shrimp paste (Ka-pi) produced in Samut Sakhon Province, Thailand	214:296	A Gram-stain-positive, aerobic, spore-forming, moderately halophilic bacterium, SSKP1-9T, was isolated from traditional salted shrimp paste (Ka-pi) produced in Samut Sakhon Province, Thailand.
31424384	2	13	attach	isolated	200:207	arg2	SSKP1-9T					186:193	SSKP1-9T	186:193	SSKP1-9T	186:193	A Gram-stain-positive, aerobic, spore-forming, moderately halophilic bacterium, SSKP1-9T, was isolated from traditional salted shrimp paste (Ka-pi) produced in Samut Sakhon Province, Thailand.
31424384	2	13	attach	isolated	200:207	arg2	bacterium					175:183	A Gram-stain-positive, aerobic, spore-forming, moderately halophilic bacterium	106:183	A Gram-stain-positive, aerobic, spore-forming, moderately halophilic bacterium	106:183	A Gram-stain-positive, aerobic, spore-forming, moderately halophilic bacterium, SSKP1-9T, was isolated from traditional salted shrimp paste (Ka-pi) produced in Samut Sakhon Province, Thailand.
31424384	2	13	attach	isolated	200:207	arg1	Ka-pi					247:251	Ka-pi	247:251	Ka-pi	247:251	A Gram-stain-positive, aerobic, spore-forming, moderately halophilic bacterium, SSKP1-9T, was isolated from traditional salted shrimp paste (Ka-pi) produced in Samut Sakhon Province, Thailand.
31424384	11	14	theme	cellular	1183:1190	arg1	anteiso-C15 					1209:1220	anteiso-C15 	1209:1220	anteiso-C15 	1209:1220	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
31424384	11	14	theme	cellular	1183:1190	arg1	acids					1198:1202	The major cellular fatty acids	1173:1202	The major cellular fatty acids	1173:1202	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
31424384	4	15	theme	gene	401:404	arg1	similarity					415:424	The 16S rRNA gene sequence similarity	388:424	The 16S rRNA gene sequence similarity values between strain SSKP1-9T and Lentibacillus juripiscarius TISTR 1535T and Lentibacillus halophilus TISTR 1549T	388:540	The 16S rRNA gene sequence similarity values between strain SSKP1-9T and Lentibacillus juripiscarius TISTR 1535T and Lentibacillus halophilus TISTR 1549T were 98.7 and 97.2 %, respectively.
31424384	5	16	theme	sequence	601:608	arg1	similarity					610:619	16S rRNA gene sequence similarity	587:619	16S rRNA gene sequence similarity	587:619	Based on 16S rRNA gene sequence similarity, strain SSKP1-9T represents a distinct novel species, as shown by phenotypic traits, DNA-DNA hybridization and average nucleotide identity values.
31424384	7	17	theme	44.6 mol	880:887	arg1	content					868:874	The genomic DNA G+C content	848:874	The genomic DNA G+C content	848:874	The genomic DNA G+C content was 44.6 mol%.
31424384	7	17	theme	44.6 mol	880:887	arg1	%					888:888	44.6 mol%	880:888	44.6 mol%	880:888	The genomic DNA G+C content was 44.6 mol%.
31424384	4	18	theme	rRNA	396:399	arg1	similarity					415:424	The 16S rRNA gene sequence similarity	388:424	The 16S rRNA gene sequence similarity values between strain SSKP1-9T and Lentibacillus juripiscarius TISTR 1535T and Lentibacillus halophilus TISTR 1549T	388:540	The 16S rRNA gene sequence similarity values between strain SSKP1-9T and Lentibacillus juripiscarius TISTR 1535T and Lentibacillus halophilus TISTR 1549T were 98.7 and 97.2 %, respectively.
31424384	12	19	theme	name	1434:1437	arg1	sp					1464:1465	the name Lentibacilluslipolyticus sp	1430:1465	the name Lentibacilluslipolyticus sp	1430:1465	The results of phenotypic and chemotaxonomic characteristics and whole-genome analysis support that strain SSKP1-9T represents a novel species of Lentibacillus, for which the name Lentibacilluslipolyticus sp.
31424384	14	20	theme	=JCM	1515:1518	arg1	SSKP1-9T					1505:1512	SSKP1-9T	1505:1512	SSKP1-9T (=JCM 32625T=TISTR 2597T)	1505:1538	The type strain is SSKP1-9T (=JCM 32625T=TISTR 2597T).
31424384	14	20	theme	=JCM	1515:1518	arg1	2597T					1533:1537	=JCM 32625T=TISTR 2597T	1515:1537	=JCM 32625T=TISTR 2597T	1515:1537	The type strain is SSKP1-9T (=JCM 32625T=TISTR 2597T).
31424384	9	21	contain	contained	958:966	arg1	peptidoglycan					944:956	The cell-wall peptidoglycan	930:956	The cell-wall peptidoglycan	930:956	The cell-wall peptidoglycan contained meso-diaminopimelic acid.
31424384	9	21	contain	contained	958:966	arg2	acid					988:991	meso-diaminopimelic acid	968:991	meso-diaminopimelic acid	968:991	The cell-wall peptidoglycan contained meso-diaminopimelic acid.
31424384	9	22	theme	cell-wall	934:942	arg1	peptidoglycan					944:956	The cell-wall peptidoglycan	930:956	The cell-wall peptidoglycan	930:956	The cell-wall peptidoglycan contained meso-diaminopimelic acid.
31424384	4	23	theme	similarity	415:424	arg1	values					426:431	The 16S rRNA gene sequence similarity values	388:431	The 16S rRNA gene sequence similarity values between strain SSKP1-9T and Lentibacillus juripiscarius TISTR 1535T and Lentibacillus halophilus TISTR 1549T	388:540	The 16S rRNA gene sequence similarity values between strain SSKP1-9T and Lentibacillus juripiscarius TISTR 1535T and Lentibacillus halophilus TISTR 1549T were 98.7 and 97.2 %, respectively.
31424384	5	24	theme	identity	751:758	arg1	values					760:765	average nucleotide identity values	732:765	average nucleotide identity values	732:765	Based on 16S rRNA gene sequence similarity, strain SSKP1-9T represents a distinct novel species, as shown by phenotypic traits, DNA-DNA hybridization and average nucleotide identity values.
31424384	14	25	theme	type	1490:1493	arg1	SSKP1-9T					1505:1512	SSKP1-9T	1505:1512	SSKP1-9T (=JCM 32625T=TISTR 2597T)	1505:1538	The type strain is SSKP1-9T (=JCM 32625T=TISTR 2597T).
31424384	14	25	theme	type	1490:1493	arg1	strain					1495:1500	The type strain	1486:1500	The type strain	1486:1500	The type strain is SSKP1-9T (=JCM 32625T=TISTR 2597T).
31424384	2	26	dep	Gram-stain-positive	108:126	arg1	halophilic					164:173	halophilic	164:173	halophilic	164:173	A Gram-stain-positive, aerobic, spore-forming, moderately halophilic bacterium, SSKP1-9T, was isolated from traditional salted shrimp paste (Ka-pi) produced in Samut Sakhon Province, Thailand.
31424384	2	26	dep	Gram-stain-positive	108:126	arg1	aerobic					129:135	aerobic	129:135	aerobic	129:135	A Gram-stain-positive, aerobic, spore-forming, moderately halophilic bacterium, SSKP1-9T, was isolated from traditional salted shrimp paste (Ka-pi) produced in Samut Sakhon Province, Thailand.
31424384	2	26	dep	Gram-stain-positive	108:126	arg1	spore-forming					138:150	spore-forming	138:150	spore-forming	138:150	A Gram-stain-positive, aerobic, spore-forming, moderately halophilic bacterium, SSKP1-9T, was isolated from traditional salted shrimp paste (Ka-pi) produced in Samut Sakhon Province, Thailand.
31424384	0	27	theme	lipolyticus	14:24	arg1	sp					26:27	Lentibacillus lipolyticus sp	0:27	Lentibacillus lipolyticus sp.	0:28	Lentibacillus lipolyticus sp.
31424384	4	28	theme	sequence	406:413	arg1	similarity					415:424	The 16S rRNA gene sequence similarity	388:424	The 16S rRNA gene sequence similarity values between strain SSKP1-9T and Lentibacillus juripiscarius TISTR 1535T and Lentibacillus halophilus TISTR 1549T	388:540	The 16S rRNA gene sequence similarity values between strain SSKP1-9T and Lentibacillus juripiscarius TISTR 1535T and Lentibacillus halophilus TISTR 1549T were 98.7 and 97.2 %, respectively.
31424384	1	29	theme	shrimp	84:89	arg1	paste					91:95	shrimp paste	84:95	shrimp paste (Ka-pi)	84:103	nov., a moderately halophilic bacterium isolated from shrimp paste (Ka-pi).
31424384	1	29	theme	shrimp	84:89	arg1	Ka-pi					98:102	Ka-pi	98:102	Ka-pi	98:102	nov., a moderately halophilic bacterium isolated from shrimp paste (Ka-pi).
31424384	4	30	dep	Lentibacillus	505:517	arg1	halophilus					519:528	halophilus	519:528	halophilus	519:528	The 16S rRNA gene sequence similarity values between strain SSKP1-9T and Lentibacillus juripiscarius TISTR 1535T and Lentibacillus halophilus TISTR 1549T were 98.7 and 97.2 %, respectively.
31424384	3	31	theme	%	374:374	arg1	NaCl					382:385	8-16 % (w/v) NaCl	369:385	8-16 % (w/v) NaCl	369:385	This strain grew optimally at 37-40 °C, pH 7.0 and in the presence of 8-16 % (w/v) NaCl.
31424384	0	32	theme	Lentibacillus	0:12	arg1	sp					26:27	Lentibacillus lipolyticus sp	0:27	Lentibacillus lipolyticus sp.	0:28	Lentibacillus lipolyticus sp.
31424384	14	33	theme	32625T=TISTR	1520:1531	arg1	SSKP1-9T					1505:1512	SSKP1-9T	1505:1512	SSKP1-9T (=JCM 32625T=TISTR 2597T)	1505:1538	The type strain is SSKP1-9T (=JCM 32625T=TISTR 2597T).
31424384	14	33	theme	32625T=TISTR	1520:1531	arg1	2597T					1533:1537	=JCM 32625T=TISTR 2597T	1515:1537	=JCM 32625T=TISTR 2597T	1515:1537	The type strain is SSKP1-9T (=JCM 32625T=TISTR 2597T).
31424384	7	34	theme	DNA	860:862	arg1	content					868:874	The genomic DNA G+C content	848:874	The genomic DNA G+C content	848:874	The genomic DNA G+C content was 44.6 mol%.
31424384	7	34	theme	DNA	860:862	arg1	%					888:888	44.6 mol%	880:888	44.6 mol%	880:888	The genomic DNA G+C content was 44.6 mol%.
31424384	11	35	theme	fatty	1192:1196	arg1	anteiso-C15 					1209:1220	anteiso-C15 	1209:1220	anteiso-C15 	1209:1220	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
31424384	11	35	theme	fatty	1192:1196	arg1	acids					1198:1202	The major cellular fatty acids	1173:1202	The major cellular fatty acids	1173:1202	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
31424384	10	36	theme	lipids	1104:1109	arg1	presence					1028:1035	the presence	1024:1035	the presence of phosphatidylglycerol, diphosphatidylglycerol, four unidentified lipids, an unidentified phospholipid and an unidentified glycolipid	1024:1170	Polar lipid analysis revealed the presence of phosphatidylglycerol, diphosphatidylglycerol, four unidentified lipids, an unidentified phospholipid and an unidentified glycolipid.
31424384	7	37	theme	G+C	864:866	arg1	content					868:874	The genomic DNA G+C content	848:874	The genomic DNA G+C content	848:874	The genomic DNA G+C content was 44.6 mol%.
31424384	7	37	theme	G+C	864:866	arg1	%					888:888	44.6 mol%	880:888	44.6 mol%	880:888	The genomic DNA G+C content was 44.6 mol%.
31424384	3	38	dep	%	374:374	arg1	w/v					377:379	w/v	377:379	w/v	377:379	This strain grew optimally at 37-40 °C, pH 7.0 and in the presence of 8-16 % (w/v) NaCl.
31424384	4	39	theme	Lentibacillus	505:517	arg1	1549T					536:540	Lentibacillus halophilus TISTR 1549T	505:540	Lentibacillus halophilus TISTR 1549T	505:540	The 16S rRNA gene sequence similarity values between strain SSKP1-9T and Lentibacillus juripiscarius TISTR 1535T and Lentibacillus halophilus TISTR 1549T were 98.7 and 97.2 %, respectively.
31424384	10	40	theme	unidentified	1091:1102	arg1	lipids					1104:1109	four unidentified lipids	1086:1109	four unidentified lipids	1086:1109	Polar lipid analysis revealed the presence of phosphatidylglycerol, diphosphatidylglycerol, four unidentified lipids, an unidentified phospholipid and an unidentified glycolipid.
31424384	11	41	theme	major	1177:1181	arg1	anteiso-C15 					1209:1220	anteiso-C15 	1209:1220	anteiso-C15 	1209:1220	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
31424384	11	41	theme	major	1177:1181	arg1	acids					1198:1202	The major cellular fatty acids	1173:1202	The major cellular fatty acids	1173:1202	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
31424384	5	42	theme	DNA-DNA	706:712	arg1	hybridization					714:726	DNA-DNA hybridization	706:726	DNA-DNA hybridization	706:726	Based on 16S rRNA gene sequence similarity, strain SSKP1-9T represents a distinct novel species, as shown by phenotypic traits, DNA-DNA hybridization and average nucleotide identity values.
31424384	1	43	attach	isolated	70:77	arg1	paste					91:95	shrimp paste	84:95	shrimp paste (Ka-pi)	84:103	nov., a moderately halophilic bacterium isolated from shrimp paste (Ka-pi).
31424384	1	43	attach	isolated	70:77	arg2	bacterium					60:68	a moderately halophilic bacterium	36:68	a moderately halophilic bacterium isolated from shrimp paste (Ka-pi)	36:103	nov., a moderately halophilic bacterium isolated from shrimp paste (Ka-pi).
31424384	1	43	attach	isolated	70:77	arg1	Ka-pi					98:102	Ka-pi	98:102	Ka-pi	98:102	nov., a moderately halophilic bacterium isolated from shrimp paste (Ka-pi).
31424384	5	44	theme	distinct	651:658	arg1	species					666:672	a distinct novel species	649:672	a distinct novel species	649:672	Based on 16S rRNA gene sequence similarity, strain SSKP1-9T represents a distinct novel species, as shown by phenotypic traits, DNA-DNA hybridization and average nucleotide identity values.
31424384	2	45	theme	salted	226:231	arg1	paste					240:244	traditional salted shrimp paste	214:244	traditional salted shrimp paste (Ka-pi) produced in Samut Sakhon Province, Thailand	214:296	A Gram-stain-positive, aerobic, spore-forming, moderately halophilic bacterium, SSKP1-9T, was isolated from traditional salted shrimp paste (Ka-pi) produced in Samut Sakhon Province, Thailand.
31424384	2	45	theme	salted	226:231	arg1	Ka-pi					247:251	Ka-pi	247:251	Ka-pi	247:251	A Gram-stain-positive, aerobic, spore-forming, moderately halophilic bacterium, SSKP1-9T, was isolated from traditional salted shrimp paste (Ka-pi) produced in Samut Sakhon Province, Thailand.
31424384	3	46	theme	NaCl	382:385	arg1	presence					357:364	the presence	353:364	the presence of 8-16 % (w/v) NaCl	353:385	This strain grew optimally at 37-40 °C, pH 7.0 and in the presence of 8-16 % (w/v) NaCl.
31424384	3	47	theme	8-16 	369:373	arg1	%					374:374	%	374:374	%	374:374	This strain grew optimally at 37-40 °C, pH 7.0 and in the presence of 8-16 % (w/v) NaCl.
31424384	4	48	theme	Lentibacillus	461:473	arg1	1535T					495:499	Lentibacillus juripiscarius TISTR 1535T	461:499	Lentibacillus juripiscarius TISTR 1535T	461:499	The 16S rRNA gene sequence similarity values between strain SSKP1-9T and Lentibacillus juripiscarius TISTR 1535T and Lentibacillus halophilus TISTR 1549T were 98.7 and 97.2 %, respectively.
31424384	5	49	theme	novel	660:664	arg1	species					666:672	a distinct novel species	649:672	a distinct novel species	649:672	Based on 16S rRNA gene sequence similarity, strain SSKP1-9T represents a distinct novel species, as shown by phenotypic traits, DNA-DNA hybridization and average nucleotide identity values.
31424384	4	50	theme	16S	392:394	arg1	similarity					415:424	The 16S rRNA gene sequence similarity	388:424	The 16S rRNA gene sequence similarity values between strain SSKP1-9T and Lentibacillus juripiscarius TISTR 1535T and Lentibacillus halophilus TISTR 1549T	388:540	The 16S rRNA gene sequence similarity values between strain SSKP1-9T and Lentibacillus juripiscarius TISTR 1535T and Lentibacillus halophilus TISTR 1549T were 98.7 and 97.2 %, respectively.
31424384	2	51	theme	traditional	214:224	arg1	paste					240:244	traditional salted shrimp paste	214:244	traditional salted shrimp paste (Ka-pi) produced in Samut Sakhon Province, Thailand	214:296	A Gram-stain-positive, aerobic, spore-forming, moderately halophilic bacterium, SSKP1-9T, was isolated from traditional salted shrimp paste (Ka-pi) produced in Samut Sakhon Province, Thailand.
31424384	2	51	theme	traditional	214:224	arg1	Ka-pi					247:251	Ka-pi	247:251	Ka-pi	247:251	A Gram-stain-positive, aerobic, spore-forming, moderately halophilic bacterium, SSKP1-9T, was isolated from traditional salted shrimp paste (Ka-pi) produced in Samut Sakhon Province, Thailand.
31424384	10	52	theme	unidentified	1115:1126	arg1	phospholipid					1128:1139	an unidentified phospholipid	1112:1139	an unidentified phospholipid	1112:1139	Polar lipid analysis revealed the presence of phosphatidylglycerol, diphosphatidylglycerol, four unidentified lipids, an unidentified phospholipid and an unidentified glycolipid.
31424384	10	53	theme	phospholipid	1128:1139	arg1	presence					1028:1035	the presence	1024:1035	the presence of phosphatidylglycerol, diphosphatidylglycerol, four unidentified lipids, an unidentified phospholipid and an unidentified glycolipid	1024:1170	Polar lipid analysis revealed the presence of phosphatidylglycerol, diphosphatidylglycerol, four unidentified lipids, an unidentified phospholipid and an unidentified glycolipid.
31424384	6	54	theme	whole-cell	785:794	arg1	profile					804:810	the whole-cell protein profile	781:810	the whole-cell protein profile	781:810	In addition, the whole-cell protein profile confirmed the novelty of the taxon.
31424384	12	55	theme	analysis	1337:1344	arg1	results					1263:1269	The results	1259:1269	The results of phenotypic and chemotaxonomic characteristics and whole-genome analysis	1259:1344	The results of phenotypic and chemotaxonomic characteristics and whole-genome analysis support that strain SSKP1-9T represents a novel species of Lentibacillus, for which the name Lentibacilluslipolyticus sp.
31424384	5	56	theme	average	732:738	arg1	values					760:765	average nucleotide identity values	732:765	average nucleotide identity values	732:765	Based on 16S rRNA gene sequence similarity, strain SSKP1-9T represents a distinct novel species, as shown by phenotypic traits, DNA-DNA hybridization and average nucleotide identity values.
31424384	4	57	theme	TISTR	489:493	arg1	1535T					495:499	Lentibacillus juripiscarius TISTR 1535T	461:499	Lentibacillus juripiscarius TISTR 1535T	461:499	The 16S rRNA gene sequence similarity values between strain SSKP1-9T and Lentibacillus juripiscarius TISTR 1535T and Lentibacillus halophilus TISTR 1549T were 98.7 and 97.2 %, respectively.
31424384	12	58	theme	whole-genome	1324:1335	arg1	analysis					1337:1344	whole-genome analysis	1324:1344	whole-genome analysis	1324:1344	The results of phenotypic and chemotaxonomic characteristics and whole-genome analysis support that strain SSKP1-9T represents a novel species of Lentibacillus, for which the name Lentibacilluslipolyticus sp.
31424384	6	59	theme	protein	796:802	arg1	profile					804:810	the whole-cell protein profile	781:810	the whole-cell protein profile	781:810	In addition, the whole-cell protein profile confirmed the novelty of the taxon.
31424384	12	60	theme	Lentibacilluslipolyticus	1439:1462	arg1	sp					1464:1465	the name Lentibacilluslipolyticus sp	1430:1465	the name Lentibacilluslipolyticus sp	1430:1465	The results of phenotypic and chemotaxonomic characteristics and whole-genome analysis support that strain SSKP1-9T represents a novel species of Lentibacillus, for which the name Lentibacilluslipolyticus sp.
31424384	5	61	theme	strain	622:627	arg1	SSKP1-9T					629:636	strain SSKP1-9T	622:636	strain SSKP1-9T	622:636	Based on 16S rRNA gene sequence similarity, strain SSKP1-9T represents a distinct novel species, as shown by phenotypic traits, DNA-DNA hybridization and average nucleotide identity values.
31424384	10	62	theme	lipid	1000:1004	arg1	analysis					1006:1013	Polar lipid analysis	994:1013	Polar lipid analysis	994:1013	Polar lipid analysis revealed the presence of phosphatidylglycerol, diphosphatidylglycerol, four unidentified lipids, an unidentified phospholipid and an unidentified glycolipid.
31424384	12	63	theme	phenotypic	1274:1283	arg1	characteristics					1304:1318	phenotypic and chemotaxonomic characteristics	1274:1318	phenotypic and chemotaxonomic characteristics	1274:1318	The results of phenotypic and chemotaxonomic characteristics and whole-genome analysis support that strain SSKP1-9T represents a novel species of Lentibacillus, for which the name Lentibacilluslipolyticus sp.
31424384	12	64	theme	characteristics	1304:1318	arg1	results					1263:1269	The results	1259:1269	The results of phenotypic and chemotaxonomic characteristics and whole-genome analysis	1259:1344	The results of phenotypic and chemotaxonomic characteristics and whole-genome analysis support that strain SSKP1-9T represents a novel species of Lentibacillus, for which the name Lentibacilluslipolyticus sp.
31424384	10	65	theme	diphosphatidylglycerol	1062:1083	arg1	presence					1028:1035	the presence	1024:1035	the presence of phosphatidylglycerol, diphosphatidylglycerol, four unidentified lipids, an unidentified phospholipid and an unidentified glycolipid	1024:1170	Polar lipid analysis revealed the presence of phosphatidylglycerol, diphosphatidylglycerol, four unidentified lipids, an unidentified phospholipid and an unidentified glycolipid.
31424384	7	66	theme	genomic	852:858	arg1	content					868:874	The genomic DNA G+C content	848:874	The genomic DNA G+C content	848:874	The genomic DNA G+C content was 44.6 mol%.
31424384	7	66	theme	genomic	852:858	arg1	%					888:888	44.6 mol%	880:888	44.6 mol%	880:888	The genomic DNA G+C content was 44.6 mol%.
31424384	12	67	theme	strain	1359:1364	arg1	SSKP1-9T					1366:1373	strain SSKP1-9T	1359:1373	strain SSKP1-9T	1359:1373	The results of phenotypic and chemotaxonomic characteristics and whole-genome analysis support that strain SSKP1-9T represents a novel species of Lentibacillus, for which the name Lentibacilluslipolyticus sp.
31424384	12	68	theme	novel	1388:1392	arg1	species					1394:1400	a novel species	1386:1400	a novel species	1386:1400	The results of phenotypic and chemotaxonomic characteristics and whole-genome analysis support that strain SSKP1-9T represents a novel species of Lentibacillus, for which the name Lentibacilluslipolyticus sp.
31424384	10	69	theme	Polar	994:998	arg1	analysis					1006:1013	Polar lipid analysis	994:1013	Polar lipid analysis	994:1013	Polar lipid analysis revealed the presence of phosphatidylglycerol, diphosphatidylglycerol, four unidentified lipids, an unidentified phospholipid and an unidentified glycolipid.
31424384	1	70	dep	bacterium	60:68	arg1	nov.					30:33	nov.	30:33	nov.	30:33	nov., a moderately halophilic bacterium isolated from shrimp paste (Ka-pi).
31424384	9	71	theme	meso-diaminopimelic	968:986	arg1	acid					988:991	meso-diaminopimelic acid	968:991	meso-diaminopimelic acid	968:991	The cell-wall peptidoglycan contained meso-diaminopimelic acid.
31424384	10	72	theme	glycolipid	1161:1170	arg1	presence					1028:1035	the presence	1024:1035	the presence of phosphatidylglycerol, diphosphatidylglycerol, four unidentified lipids, an unidentified phospholipid and an unidentified glycolipid	1024:1170	Polar lipid analysis revealed the presence of phosphatidylglycerol, diphosphatidylglycerol, four unidentified lipids, an unidentified phospholipid and an unidentified glycolipid.
31424384	12	73	theme	chemotaxonomic	1289:1302	arg1	characteristics					1304:1318	phenotypic and chemotaxonomic characteristics	1274:1318	phenotypic and chemotaxonomic characteristics	1274:1318	The results of phenotypic and chemotaxonomic characteristics and whole-genome analysis support that strain SSKP1-9T represents a novel species of Lentibacillus, for which the name Lentibacilluslipolyticus sp.
31424384	6	74	theme	taxon	841:845	arg1	novelty					826:832	the novelty	822:832	the novelty of the taxon	822:845	In addition, the whole-cell protein profile confirmed the novelty of the taxon.
31424384	5	75	theme	phenotypic	687:696	arg1	traits					698:703	phenotypic traits	687:703	phenotypic traits	687:703	Based on 16S rRNA gene sequence similarity, strain SSKP1-9T represents a distinct novel species, as shown by phenotypic traits, DNA-DNA hybridization and average nucleotide identity values.
31424384	2	76	theme	Gram-stain-positive	108:126	arg1	SSKP1-9T					186:193	SSKP1-9T	186:193	SSKP1-9T	186:193	A Gram-stain-positive, aerobic, spore-forming, moderately halophilic bacterium, SSKP1-9T, was isolated from traditional salted shrimp paste (Ka-pi) produced in Samut Sakhon Province, Thailand.
31424384	2	76	theme	Gram-stain-positive	108:126	arg1	bacterium					175:183	A Gram-stain-positive, aerobic, spore-forming, moderately halophilic bacterium	106:183	A Gram-stain-positive, aerobic, spore-forming, moderately halophilic bacterium	106:183	A Gram-stain-positive, aerobic, spore-forming, moderately halophilic bacterium, SSKP1-9T, was isolated from traditional salted shrimp paste (Ka-pi) produced in Samut Sakhon Province, Thailand.
31424384	2	77	theme	shrimp	233:238	arg1	paste					240:244	traditional salted shrimp paste	214:244	traditional salted shrimp paste (Ka-pi) produced in Samut Sakhon Province, Thailand	214:296	A Gram-stain-positive, aerobic, spore-forming, moderately halophilic bacterium, SSKP1-9T, was isolated from traditional salted shrimp paste (Ka-pi) produced in Samut Sakhon Province, Thailand.
31424384	2	77	theme	shrimp	233:238	arg1	Ka-pi					247:251	Ka-pi	247:251	Ka-pi	247:251	A Gram-stain-positive, aerobic, spore-forming, moderately halophilic bacterium, SSKP1-9T, was isolated from traditional salted shrimp paste (Ka-pi) produced in Samut Sakhon Province, Thailand.
31424384	5	78	theme	nucleotide	740:749	arg1	values					760:765	average nucleotide identity values	732:765	average nucleotide identity values	732:765	Based on 16S rRNA gene sequence similarity, strain SSKP1-9T represents a distinct novel species, as shown by phenotypic traits, DNA-DNA hybridization and average nucleotide identity values.
31225554	0	0	theme	microbiome-metabolomics	71:93	arg1	analysis					95:102	microbiome-metabolomics analysis	71:102	microbiome-metabolomics analysis	71:102	A holistic view of gallic acid-induced attenuation in colitis based on microbiome-metabolomics analysis.
31225554	0	1	from	view	11:14	arg1	colitis					54:60	colitis	54:60	colitis	54:60	A holistic view of gallic acid-induced attenuation in colitis based on microbiome-metabolomics analysis.
31225554	11	2	from	profiles	1814:1821	arg1	DSS-colitis					1826:1836	DSS-colitis	1826:1836	DSS-colitis	1826:1836	These findings demonstrate GA-induced alterations in metabolic and bacterial profiles in DSS-colitis, providing new insight into the attenuation of GA in UC.
31225554	2	3	from	mechanism	248:256	arg1	UC					267:268	UC	267:268	UC	267:268	However, the mechanism of GA in UC remains unknown.
31225554	7	4	theme	GA	980:981	arg1	group					983:987	the GA group	976:987	the GA group	976:987	Our results showed that UC syndromes in the GA group were significantly attenuated.
31225554	8	5	theme	microbial	1024:1032	arg1	alterations					1034:1044	The microbial alterations	1020:1044	The microbial alterations in the DSS group	1020:1061	The microbial alterations in the DSS group were characterized by a decrease of probiotic bacteria, such as Lactobacillaceae and Prevotellaceae, and an increase of some pathogenic species, mainly in the Firmicutes and Proteobacteria phyla.
31225554	4	6	theme	-induced	586:593	arg1	colitis					595:601	dextra sulfate sodium (DSS)-induced colitis	559:601	dextra sulfate sodium (DSS)-induced colitis	559:601	Rats with dextra sulfate sodium (DSS)-induced colitis were rectally administrated with GA (6 mg kg-1) for 8 consecutive days.
31225554	10	7	theme	carbohydrate	1455:1466	arg1	metabolism					1468:1477	carbohydrate metabolism	1455:1477	carbohydrate metabolism (gluco-related metabolism)	1455:1504	Metabolic data further revealed that the GA-induced metabolic changes focus on increasing carbohydrate metabolism (gluco-related metabolism) and bile acid (BA) metabolism and decreasing amino acid metabolism, which also provides evidence for alteration of the microbiota because these feces metabolites are by-products of interactions between the host and the microbiota.
31225554	10	7	theme	carbohydrate	1455:1466	arg1	metabolism					1494:1503	gluco-related metabolism	1480:1503	gluco-related metabolism	1480:1503	Metabolic data further revealed that the GA-induced metabolic changes focus on increasing carbohydrate metabolism (gluco-related metabolism) and bile acid (BA) metabolism and decreasing amino acid metabolism, which also provides evidence for alteration of the microbiota because these feces metabolites are by-products of interactions between the host and the microbiota.
31225554	10	8	theme	amino	1551:1555	arg1	metabolism					1562:1571	amino acid metabolism	1551:1571	amino acid metabolism	1551:1571	Metabolic data further revealed that the GA-induced metabolic changes focus on increasing carbohydrate metabolism (gluco-related metabolism) and bile acid (BA) metabolism and decreasing amino acid metabolism, which also provides evidence for alteration of the microbiota because these feces metabolites are by-products of interactions between the host and the microbiota.
31225554	8	9	theme	species	1199:1205	arg1	increase					1171:1178	an increase	1168:1178	an increase of some pathogenic species	1168:1205	The microbial alterations in the DSS group were characterized by a decrease of probiotic bacteria, such as Lactobacillaceae and Prevotellaceae, and an increase of some pathogenic species, mainly in the Firmicutes and Proteobacteria phyla.
31225554	8	9	theme	species	1199:1205	arg1	decrease					1087:1094	a decrease	1085:1094	a decrease of probiotic bacteria, such as Lactobacillaceae and Prevotellaceae,	1085:1162	The microbial alterations in the DSS group were characterized by a decrease of probiotic bacteria, such as Lactobacillaceae and Prevotellaceae, and an increase of some pathogenic species, mainly in the Firmicutes and Proteobacteria phyla.
31225554	6	10	theme	feces	779:783	arg1	samples					785:791	Urine and feces samples	769:791	Urine and feces samples	769:791	Urine and feces samples were analyzed with 1H NMR spectroscopy, and short chain fatty acids (SCFAs) in feces and colon contents were detected with gas chromatography.
31225554	10	11	theme	microbiota	1625:1634	arg1	alteration					1607:1616	alteration	1607:1616	alteration of the microbiota	1607:1634	Metabolic data further revealed that the GA-induced metabolic changes focus on increasing carbohydrate metabolism (gluco-related metabolism) and bile acid (BA) metabolism and decreasing amino acid metabolism, which also provides evidence for alteration of the microbiota because these feces metabolites are by-products of interactions between the host and the microbiota.
31225554	11	12	from	alterations	1775:1785	arg1	profiles					1814:1821	metabolic and bacterial profiles	1790:1821	metabolic and bacterial profiles in DSS-colitis	1790:1836	These findings demonstrate GA-induced alterations in metabolic and bacterial profiles in DSS-colitis, providing new insight into the attenuation of GA in UC.
31225554	8	13	theme	pathogenic	1188:1197	arg1	species					1199:1205	some pathogenic species	1183:1205	some pathogenic species	1183:1205	The microbial alterations in the DSS group were characterized by a decrease of probiotic bacteria, such as Lactobacillaceae and Prevotellaceae, and an increase of some pathogenic species, mainly in the Firmicutes and Proteobacteria phyla.
31225554	3	14	from	functions	526:534	arg1	UC					545:546	UC	545:546	UC	545:546	In this study, we investigated the therapeutic effects of GA on UC from the perspective of gut microbiota and supervised the metabolic alterations in vivo with 1H NMR-based metabolomics, which can provide a holistic view to understand the functions of GA in UC.
31225554	9	15	theme	control	1350:1356	arg1	group					1358:1362	the control group	1346:1362	the control group	1346:1362	GA treatment could modulate the microbiota composition towards a similar proportion to the control group.
31225554	11	16	from	attenuation	1870:1880	arg1	UC					1891:1892	UC	1891:1892	UC	1891:1892	These findings demonstrate GA-induced alterations in metabolic and bacterial profiles in DSS-colitis, providing new insight into the attenuation of GA in UC.
31225554	5	17	theme	gene	679:682	arg1	sequencing					684:693	16S gene sequencing	675:693	16S gene sequencing	675:693	16S gene sequencing was performed on feces samples to obtain bacterial community information.
31225554	3	18	theme	NMR-based	450:458	arg1	metabolomics					460:471	1H NMR-based metabolomics	447:471	1H NMR-based metabolomics	447:471	In this study, we investigated the therapeutic effects of GA on UC from the perspective of gut microbiota and supervised the metabolic alterations in vivo with 1H NMR-based metabolomics, which can provide a holistic view to understand the functions of GA in UC.
31225554	8	19	theme	bacteria	1109:1116	arg1	increase					1171:1178	an increase	1168:1178	an increase of some pathogenic species	1168:1205	The microbial alterations in the DSS group were characterized by a decrease of probiotic bacteria, such as Lactobacillaceae and Prevotellaceae, and an increase of some pathogenic species, mainly in the Firmicutes and Proteobacteria phyla.
31225554	8	19	theme	bacteria	1109:1116	arg1	decrease					1087:1094	a decrease	1085:1094	a decrease of probiotic bacteria, such as Lactobacillaceae and Prevotellaceae,	1085:1162	The microbial alterations in the DSS group were characterized by a decrease of probiotic bacteria, such as Lactobacillaceae and Prevotellaceae, and an increase of some pathogenic species, mainly in the Firmicutes and Proteobacteria phyla.
31225554	9	20	theme	microbiota	1291:1300	arg1	composition					1302:1312	the microbiota composition	1287:1312	the microbiota composition	1287:1312	GA treatment could modulate the microbiota composition towards a similar proportion to the control group.
31225554	5	21	theme	16S	675:677	arg1	sequencing					684:693	16S gene sequencing	675:693	16S gene sequencing	675:693	16S gene sequencing was performed on feces samples to obtain bacterial community information.
31225554	1	22	located	found	138:142	arg2	acid					112:115	Gallic acid	105:115	Gallic acid (GA)	105:120	Gallic acid (GA), a plant phenol found in fruits and vegetables, has been recently reported to attenuate ulcerative colitis (UC).
31225554	1	22	located	found	138:142	arg2	phenol					131:136	a plant phenol	123:136	a plant phenol found in fruits and vegetables	123:167	Gallic acid (GA), a plant phenol found in fruits and vegetables, has been recently reported to attenuate ulcerative colitis (UC).
31225554	1	22	located	found	138:142	arg1	fruits					147:152	fruits	147:152	fruits	147:152	Gallic acid (GA), a plant phenol found in fruits and vegetables, has been recently reported to attenuate ulcerative colitis (UC).
31225554	1	22	located	found	138:142	arg1	vegetables					158:167	vegetables	158:167	vegetables	158:167	Gallic acid (GA), a plant phenol found in fruits and vegetables, has been recently reported to attenuate ulcerative colitis (UC).
31225554	3	23	theme	GA	345:346	arg1	effects					334:340	the therapeutic effects	318:340	the therapeutic effects of GA on UC	318:352	In this study, we investigated the therapeutic effects of GA on UC from the perspective of gut microbiota and supervised the metabolic alterations in vivo with 1H NMR-based metabolomics, which can provide a holistic view to understand the functions of GA in UC.
31225554	10	24	theme	gluco-related	1480:1492	arg1	metabolism					1468:1477	carbohydrate metabolism	1455:1477	carbohydrate metabolism (gluco-related metabolism)	1455:1504	Metabolic data further revealed that the GA-induced metabolic changes focus on increasing carbohydrate metabolism (gluco-related metabolism) and bile acid (BA) metabolism and decreasing amino acid metabolism, which also provides evidence for alteration of the microbiota because these feces metabolites are by-products of interactions between the host and the microbiota.
31225554	10	24	theme	gluco-related	1480:1492	arg1	metabolism					1494:1503	gluco-related metabolism	1480:1503	gluco-related metabolism	1480:1503	Metabolic data further revealed that the GA-induced metabolic changes focus on increasing carbohydrate metabolism (gluco-related metabolism) and bile acid (BA) metabolism and decreasing amino acid metabolism, which also provides evidence for alteration of the microbiota because these feces metabolites are by-products of interactions between the host and the microbiota.
31225554	11	25	from	GA	1885:1886	arg1	UC					1891:1892	UC	1891:1892	UC	1891:1892	These findings demonstrate GA-induced alterations in metabolic and bacterial profiles in DSS-colitis, providing new insight into the attenuation of GA in UC.
31225554	5	26	theme	bacterial	736:744	arg1	information					756:766	bacterial community information	736:766	bacterial community information	736:766	16S gene sequencing was performed on feces samples to obtain bacterial community information.
31225554	0	27	theme	holistic	2:9	arg1	view					11:14	A holistic view	0:14	A holistic view of gallic acid-induced attenuation in colitis	0:60	A holistic view of gallic acid-induced attenuation in colitis based on microbiome-metabolomics analysis.
31225554	11	28	theme	metabolic	1790:1798	arg1	profiles					1814:1821	metabolic and bacterial profiles	1790:1821	metabolic and bacterial profiles in DSS-colitis	1790:1836	These findings demonstrate GA-induced alterations in metabolic and bacterial profiles in DSS-colitis, providing new insight into the attenuation of GA in UC.
31225554	5	29	theme	community	746:754	arg1	information					756:766	bacterial community information	736:766	bacterial community information	736:766	16S gene sequencing was performed on feces samples to obtain bacterial community information.
31225554	3	30	theme	therapeutic	322:332	arg1	effects					334:340	the therapeutic effects	318:340	the therapeutic effects of GA on UC	318:352	In this study, we investigated the therapeutic effects of GA on UC from the perspective of gut microbiota and supervised the metabolic alterations in vivo with 1H NMR-based metabolomics, which can provide a holistic view to understand the functions of GA in UC.
31225554	6	31	theme	fatty	849:853	arg1	SCFAs					862:866	SCFAs	862:866	SCFAs	862:866	Urine and feces samples were analyzed with 1H NMR spectroscopy, and short chain fatty acids (SCFAs) in feces and colon contents were detected with gas chromatography.
31225554	6	31	theme	fatty	849:853	arg1	acids					855:859	short chain fatty acids	837:859	short chain fatty acids (SCFAs) in feces and colon contents	837:895	Urine and feces samples were analyzed with 1H NMR spectroscopy, and short chain fatty acids (SCFAs) in feces and colon contents were detected with gas chromatography.
31225554	3	32	theme	holistic	494:501	arg1	view					503:506	a holistic view	492:506	a holistic view to understand the functions of GA in UC	492:546	In this study, we investigated the therapeutic effects of GA on UC from the perspective of gut microbiota and supervised the metabolic alterations in vivo with 1H NMR-based metabolomics, which can provide a holistic view to understand the functions of GA in UC.
31225554	6	33	theme	gas	916:918	arg1	chromatography					920:933	gas chromatography	916:933	gas chromatography	916:933	Urine and feces samples were analyzed with 1H NMR spectroscopy, and short chain fatty acids (SCFAs) in feces and colon contents were detected with gas chromatography.
31225554	6	34	theme	chain	843:847	arg1	SCFAs					862:866	SCFAs	862:866	SCFAs	862:866	Urine and feces samples were analyzed with 1H NMR spectroscopy, and short chain fatty acids (SCFAs) in feces and colon contents were detected with gas chromatography.
31225554	6	34	theme	chain	843:847	arg1	acids					855:859	short chain fatty acids	837:859	short chain fatty acids (SCFAs) in feces and colon contents	837:895	Urine and feces samples were analyzed with 1H NMR spectroscopy, and short chain fatty acids (SCFAs) in feces and colon contents were detected with gas chromatography.
31225554	10	35	theme	Metabolic	1365:1373	arg1	data					1375:1378	Metabolic data	1365:1378	Metabolic data	1365:1378	Metabolic data further revealed that the GA-induced metabolic changes focus on increasing carbohydrate metabolism (gluco-related metabolism) and bile acid (BA) metabolism and decreasing amino acid metabolism, which also provides evidence for alteration of the microbiota because these feces metabolites are by-products of interactions between the host and the microbiota.
31225554	1	36	theme	Gallic	105:110	arg1	phenol					131:136	a plant phenol	123:136	a plant phenol found in fruits and vegetables	123:167	Gallic acid (GA), a plant phenol found in fruits and vegetables, has been recently reported to attenuate ulcerative colitis (UC).
31225554	1	36	theme	Gallic	105:110	arg1	GA					118:119	GA	118:119	GA	118:119	Gallic acid (GA), a plant phenol found in fruits and vegetables, has been recently reported to attenuate ulcerative colitis (UC).
31225554	1	36	theme	Gallic	105:110	arg1	acid					112:115	Gallic acid	105:115	Gallic acid (GA)	105:120	Gallic acid (GA), a plant phenol found in fruits and vegetables, has been recently reported to attenuate ulcerative colitis (UC).
31225554	7	37	theme	UC	960:961	arg1	syndromes					963:971	UC syndromes	960:971	UC syndromes in the GA group	960:987	Our results showed that UC syndromes in the GA group were significantly attenuated.
31225554	3	38	theme	microbiota	382:391	arg1	perspective					363:373	the perspective	359:373	the perspective of gut microbiota	359:391	In this study, we investigated the therapeutic effects of GA on UC from the perspective of gut microbiota and supervised the metabolic alterations in vivo with 1H NMR-based metabolomics, which can provide a holistic view to understand the functions of GA in UC.
31225554	2	39	theme	GA	261:262	arg1	mechanism					248:256	the mechanism	244:256	the mechanism of GA in UC	244:268	However, the mechanism of GA in UC remains unknown.
31225554	6	40	theme	short	837:841	arg1	SCFAs					862:866	SCFAs	862:866	SCFAs	862:866	Urine and feces samples were analyzed with 1H NMR spectroscopy, and short chain fatty acids (SCFAs) in feces and colon contents were detected with gas chromatography.
31225554	6	40	theme	short	837:841	arg1	acids					855:859	short chain fatty acids	837:859	short chain fatty acids (SCFAs) in feces and colon contents	837:895	Urine and feces samples were analyzed with 1H NMR spectroscopy, and short chain fatty acids (SCFAs) in feces and colon contents were detected with gas chromatography.
31225554	11	41	theme	GA-induced	1764:1773	arg1	alterations					1775:1785	GA-induced alterations	1764:1785	GA-induced alterations in metabolic and bacterial profiles in DSS-colitis	1764:1836	These findings demonstrate GA-induced alterations in metabolic and bacterial profiles in DSS-colitis, providing new insight into the attenuation of GA in UC.
31225554	10	42	theme	GA-induced	1406:1415	arg1	changes					1427:1433	the GA-induced metabolic changes	1402:1433	the GA-induced metabolic changes	1402:1433	Metabolic data further revealed that the GA-induced metabolic changes focus on increasing carbohydrate metabolism (gluco-related metabolism) and bile acid (BA) metabolism and decreasing amino acid metabolism, which also provides evidence for alteration of the microbiota because these feces metabolites are by-products of interactions between the host and the microbiota.
31225554	5	43	theme	feces	712:716	arg1	samples					718:724	feces samples	712:724	feces samples	712:724	16S gene sequencing was performed on feces samples to obtain bacterial community information.
31225554	0	44	theme	acid-induced	26:37	arg1	attenuation					39:49	gallic acid-induced attenuation	19:49	gallic acid-induced attenuation	19:49	A holistic view of gallic acid-induced attenuation in colitis based on microbiome-metabolomics analysis.
31225554	10	45	theme	feces	1650:1654	arg1	by-products					1672:1682	by-products	1672:1682	by-products of interactions between the host and the microbiota	1672:1734	Metabolic data further revealed that the GA-induced metabolic changes focus on increasing carbohydrate metabolism (gluco-related metabolism) and bile acid (BA) metabolism and decreasing amino acid metabolism, which also provides evidence for alteration of the microbiota because these feces metabolites are by-products of interactions between the host and the microbiota.
31225554	10	45	theme	feces	1650:1654	arg1	metabolites					1656:1666	these feces metabolites	1644:1666	these feces metabolites	1644:1666	Metabolic data further revealed that the GA-induced metabolic changes focus on increasing carbohydrate metabolism (gluco-related metabolism) and bile acid (BA) metabolism and decreasing amino acid metabolism, which also provides evidence for alteration of the microbiota because these feces metabolites are by-products of interactions between the host and the microbiota.
31225554	10	46	theme	acid	1557:1560	arg1	metabolism					1562:1571	amino acid metabolism	1551:1571	amino acid metabolism	1551:1571	Metabolic data further revealed that the GA-induced metabolic changes focus on increasing carbohydrate metabolism (gluco-related metabolism) and bile acid (BA) metabolism and decreasing amino acid metabolism, which also provides evidence for alteration of the microbiota because these feces metabolites are by-products of interactions between the host and the microbiota.
31225554	8	47	theme	probiotic	1099:1107	arg1	bacteria					1109:1116	probiotic bacteria	1099:1116	probiotic bacteria	1099:1116	The microbial alterations in the DSS group were characterized by a decrease of probiotic bacteria, such as Lactobacillaceae and Prevotellaceae, and an increase of some pathogenic species, mainly in the Firmicutes and Proteobacteria phyla.
31225554	8	47	theme	probiotic	1099:1107	arg1	Prevotellaceae					1148:1161	Prevotellaceae	1148:1161	Prevotellaceae	1148:1161	The microbial alterations in the DSS group were characterized by a decrease of probiotic bacteria, such as Lactobacillaceae and Prevotellaceae, and an increase of some pathogenic species, mainly in the Firmicutes and Proteobacteria phyla.
31225554	8	47	theme	probiotic	1099:1107	arg1	Lactobacillaceae					1127:1142	Lactobacillaceae	1127:1142	Lactobacillaceae	1127:1142	The microbial alterations in the DSS group were characterized by a decrease of probiotic bacteria, such as Lactobacillaceae and Prevotellaceae, and an increase of some pathogenic species, mainly in the Firmicutes and Proteobacteria phyla.
31225554	0	48	theme	gallic	19:24	arg1	attenuation					39:49	gallic acid-induced attenuation	19:49	gallic acid-induced attenuation	19:49	A holistic view of gallic acid-induced attenuation in colitis based on microbiome-metabolomics analysis.
31225554	7	49	from	syndromes	963:971	arg1	group					983:987	the GA group	976:987	the GA group	976:987	Our results showed that UC syndromes in the GA group were significantly attenuated.
31225554	10	50	theme	bile	1510:1513	arg1	BA					1521:1522	BA	1521:1522	BA	1521:1522	Metabolic data further revealed that the GA-induced metabolic changes focus on increasing carbohydrate metabolism (gluco-related metabolism) and bile acid (BA) metabolism and decreasing amino acid metabolism, which also provides evidence for alteration of the microbiota because these feces metabolites are by-products of interactions between the host and the microbiota.
31225554	10	50	theme	bile	1510:1513	arg1	acid					1515:1518	bile acid	1510:1518	bile acid (BA) metabolism	1510:1534	Metabolic data further revealed that the GA-induced metabolic changes focus on increasing carbohydrate metabolism (gluco-related metabolism) and bile acid (BA) metabolism and decreasing amino acid metabolism, which also provides evidence for alteration of the microbiota because these feces metabolites are by-products of interactions between the host and the microbiota.
31225554	8	51	theme	Proteobacteria	1237:1250	arg1	phyla					1252:1256	Proteobacteria phyla	1237:1256	Proteobacteria phyla	1237:1256	The microbial alterations in the DSS group were characterized by a decrease of probiotic bacteria, such as Lactobacillaceae and Prevotellaceae, and an increase of some pathogenic species, mainly in the Firmicutes and Proteobacteria phyla.
31225554	3	52	theme	gut	378:380	arg1	microbiota					382:391	gut microbiota	378:391	gut microbiota	378:391	In this study, we investigated the therapeutic effects of GA on UC from the perspective of gut microbiota and supervised the metabolic alterations in vivo with 1H NMR-based metabolomics, which can provide a holistic view to understand the functions of GA in UC.
31225554	11	53	theme	GA	1885:1886	arg1	attenuation					1870:1880	the attenuation	1866:1880	the attenuation of GA in UC	1866:1892	These findings demonstrate GA-induced alterations in metabolic and bacterial profiles in DSS-colitis, providing new insight into the attenuation of GA in UC.
31225554	11	54	theme	new	1849:1851	arg1	insight					1853:1859	new insight	1849:1859	new insight into the attenuation of GA in UC	1849:1892	These findings demonstrate GA-induced alterations in metabolic and bacterial profiles in DSS-colitis, providing new insight into the attenuation of GA in UC.
31225554	6	55	theme	Urine	769:773	arg1	samples					785:791	Urine and feces samples	769:791	Urine and feces samples	769:791	Urine and feces samples were analyzed with 1H NMR spectroscopy, and short chain fatty acids (SCFAs) in feces and colon contents were detected with gas chromatography.
31225554	3	56	theme	metabolic	412:420	arg1	alterations					422:432	the metabolic alterations	408:432	the metabolic alterations	408:432	In this study, we investigated the therapeutic effects of GA on UC from the perspective of gut microbiota and supervised the metabolic alterations in vivo with 1H NMR-based metabolomics, which can provide a holistic view to understand the functions of GA in UC.
31225554	0	57	theme	attenuation	39:49	arg1	view					11:14	A holistic view	0:14	A holistic view of gallic acid-induced attenuation in colitis	0:60	A holistic view of gallic acid-induced attenuation in colitis based on microbiome-metabolomics analysis.
31225554	6	58	theme	NMR	815:817	arg1	spectroscopy					819:830	1H NMR spectroscopy	812:830	1H NMR spectroscopy	812:830	Urine and feces samples were analyzed with 1H NMR spectroscopy, and short chain fatty acids (SCFAs) in feces and colon contents were detected with gas chromatography.
31225554	8	59	from	alterations	1034:1044	arg1	group					1057:1061	the DSS group	1049:1061	the DSS group	1049:1061	The microbial alterations in the DSS group were characterized by a decrease of probiotic bacteria, such as Lactobacillaceae and Prevotellaceae, and an increase of some pathogenic species, mainly in the Firmicutes and Proteobacteria phyla.
31225554	6	60	from	acids	855:859	arg1	feces					872:876	feces	872:876	feces	872:876	Urine and feces samples were analyzed with 1H NMR spectroscopy, and short chain fatty acids (SCFAs) in feces and colon contents were detected with gas chromatography.
31225554	6	60	from	acids	855:859	arg1	contents					888:895	colon contents	882:895	colon contents	882:895	Urine and feces samples were analyzed with 1H NMR spectroscopy, and short chain fatty acids (SCFAs) in feces and colon contents were detected with gas chromatography.
31225554	6	61	theme	1H	812:813	arg1	spectroscopy					819:830	1H NMR spectroscopy	812:830	1H NMR spectroscopy	812:830	Urine and feces samples were analyzed with 1H NMR spectroscopy, and short chain fatty acids (SCFAs) in feces and colon contents were detected with gas chromatography.
31225554	10	62	theme	acid	1515:1518	arg1	metabolism					1525:1534	bile acid (BA) metabolism	1510:1534	bile acid (BA) metabolism	1510:1534	Metabolic data further revealed that the GA-induced metabolic changes focus on increasing carbohydrate metabolism (gluco-related metabolism) and bile acid (BA) metabolism and decreasing amino acid metabolism, which also provides evidence for alteration of the microbiota because these feces metabolites are by-products of interactions between the host and the microbiota.
31225554	4	63	with	Rats	549:552	arg1	colitis					595:601	dextra sulfate sodium (DSS)-induced colitis	559:601	dextra sulfate sodium (DSS)-induced colitis	559:601	Rats with dextra sulfate sodium (DSS)-induced colitis were rectally administrated with GA (6 mg kg-1) for 8 consecutive days.
31225554	3	64	theme	GA	539:540	arg1	functions					526:534	the functions	522:534	the functions of GA in UC	522:546	In this study, we investigated the therapeutic effects of GA on UC from the perspective of gut microbiota and supervised the metabolic alterations in vivo with 1H NMR-based metabolomics, which can provide a holistic view to understand the functions of GA in UC.
31225554	10	65	theme	interactions	1687:1698	arg1	by-products					1672:1682	by-products	1672:1682	by-products of interactions between the host and the microbiota	1672:1734	Metabolic data further revealed that the GA-induced metabolic changes focus on increasing carbohydrate metabolism (gluco-related metabolism) and bile acid (BA) metabolism and decreasing amino acid metabolism, which also provides evidence for alteration of the microbiota because these feces metabolites are by-products of interactions between the host and the microbiota.
31225554	10	65	theme	interactions	1687:1698	arg1	metabolites					1656:1666	these feces metabolites	1644:1666	these feces metabolites	1644:1666	Metabolic data further revealed that the GA-induced metabolic changes focus on increasing carbohydrate metabolism (gluco-related metabolism) and bile acid (BA) metabolism and decreasing amino acid metabolism, which also provides evidence for alteration of the microbiota because these feces metabolites are by-products of interactions between the host and the microbiota.
31225554	4	66	theme	mg	642:643	arg1	GA					636:637	GA	636:637	GA (6 mg kg-1)	636:649	Rats with dextra sulfate sodium (DSS)-induced colitis were rectally administrated with GA (6 mg kg-1) for 8 consecutive days.
31225554	4	66	theme	mg	642:643	arg1	kg-1					645:648	6 mg kg-1	640:648	6 mg kg-1	640:648	Rats with dextra sulfate sodium (DSS)-induced colitis were rectally administrated with GA (6 mg kg-1) for 8 consecutive days.
31225554	6	67	theme	colon	882:886	arg1	contents					888:895	colon contents	882:895	colon contents	882:895	Urine and feces samples were analyzed with 1H NMR spectroscopy, and short chain fatty acids (SCFAs) in feces and colon contents were detected with gas chromatography.
31225554	11	68	from	UC	1891:1892	arg1	attenuation					1870:1880	the attenuation	1866:1880	the attenuation of GA in UC	1866:1892	These findings demonstrate GA-induced alterations in metabolic and bacterial profiles in DSS-colitis, providing new insight into the attenuation of GA in UC.
31225554	4	69	theme	consecutive	657:667	arg1	days					669:672	8 consecutive days	655:672	8 consecutive days	655:672	Rats with dextra sulfate sodium (DSS)-induced colitis were rectally administrated with GA (6 mg kg-1) for 8 consecutive days.
31225554	1	70	theme	plant	125:129	arg1	phenol					131:136	a plant phenol	123:136	a plant phenol found in fruits and vegetables	123:167	Gallic acid (GA), a plant phenol found in fruits and vegetables, has been recently reported to attenuate ulcerative colitis (UC).
31225554	1	70	theme	plant	125:129	arg1	acid					112:115	Gallic acid	105:115	Gallic acid (GA)	105:120	Gallic acid (GA), a plant phenol found in fruits and vegetables, has been recently reported to attenuate ulcerative colitis (UC).
31225554	9	71	theme	similar	1324:1330	arg1	proportion					1332:1341	a similar proportion	1322:1341	a similar proportion to the control group	1322:1362	GA treatment could modulate the microbiota composition towards a similar proportion to the control group.
31225554	11	72	theme	bacterial	1804:1812	arg1	profiles					1814:1821	metabolic and bacterial profiles	1790:1821	metabolic and bacterial profiles in DSS-colitis	1790:1836	These findings demonstrate GA-induced alterations in metabolic and bacterial profiles in DSS-colitis, providing new insight into the attenuation of GA in UC.
31225554	10	73	theme	metabolic	1417:1425	arg1	changes					1427:1433	the GA-induced metabolic changes	1402:1433	the GA-induced metabolic changes	1402:1433	Metabolic data further revealed that the GA-induced metabolic changes focus on increasing carbohydrate metabolism (gluco-related metabolism) and bile acid (BA) metabolism and decreasing amino acid metabolism, which also provides evidence for alteration of the microbiota because these feces metabolites are by-products of interactions between the host and the microbiota.
31225554	3	74	from	effects	334:340	arg1	UC					351:352	UC	351:352	UC	351:352	In this study, we investigated the therapeutic effects of GA on UC from the perspective of gut microbiota and supervised the metabolic alterations in vivo with 1H NMR-based metabolomics, which can provide a holistic view to understand the functions of GA in UC.
31225554	8	75	theme	DSS	1053:1055	arg1	group					1057:1061	the DSS group	1049:1061	the DSS group	1049:1061	The microbial alterations in the DSS group were characterized by a decrease of probiotic bacteria, such as Lactobacillaceae and Prevotellaceae, and an increase of some pathogenic species, mainly in the Firmicutes and Proteobacteria phyla.
31225554	3	76	theme	1H	447:448	arg1	metabolomics					460:471	1H NMR-based metabolomics	447:471	1H NMR-based metabolomics	447:471	In this study, we investigated the therapeutic effects of GA on UC from the perspective of gut microbiota and supervised the metabolic alterations in vivo with 1H NMR-based metabolomics, which can provide a holistic view to understand the functions of GA in UC.
31225554	9	77	theme	GA	1259:1260	arg1	treatment					1262:1270	GA treatment	1259:1270	GA treatment	1259:1270	GA treatment could modulate the microbiota composition towards a similar proportion to the control group.
31225554	1	78	theme	ulcerative	210:219	arg1	UC					230:231	UC	230:231	UC	230:231	Gallic acid (GA), a plant phenol found in fruits and vegetables, has been recently reported to attenuate ulcerative colitis (UC).
31225554	1	78	theme	ulcerative	210:219	arg1	colitis					221:227	ulcerative colitis	210:227	ulcerative colitis (UC)	210:232	Gallic acid (GA), a plant phenol found in fruits and vegetables, has been recently reported to attenuate ulcerative colitis (UC).
31029001	5	0	theme	elevated	847:854	arg1	temperature					863:873	the elevated rectal temperature	843:873	the elevated rectal temperature in mice	843:881	The extract showed a substantial antipyretic activity as it decreased significantly the elevated rectal temperature in mice after intraperitoneal injection of Brewer's yeast.
31029001	1	1	theme	current	143:149	arg1	work					151:154	the current work	139:154	the current work	139:154	In the current work, the phytochemical composition of a leaf methanol extract from Albizia anthelmintica was thoroughly investigated.
31029001	4	2	theme	central	587:593	arg1	effects					627:633	central and peripheral anti-nociceptive effects	587:633	central and peripheral anti-nociceptive effects	587:633	The extract exhibited central and peripheral anti-nociceptive effects; it significantly decreased the number of acetic acid induced writhes and prolonged the latency time in the hot plate test.
31029001	6	3	from	docking	944:950	arg1	extract					983:989	the extract	979:989	the extract	979:989	Molecular docking of some major compounds in the extract to COX-1, COX-2 and 5-LOX, enzymes involved in the inflammation cascade, revealed appreciable interactions with the conserved amino acid residues in these target proteins.
31029001	6	4	theme	Molecular	934:942	arg1	docking					944:950	Molecular docking	934:950	Molecular docking of some major compounds in the extract to COX-1, COX-2 and 5-LOX, enzymes involved in the inflammation cascade,	934:1062	Molecular docking of some major compounds in the extract to COX-1, COX-2 and 5-LOX, enzymes involved in the inflammation cascade, revealed appreciable interactions with the conserved amino acid residues in these target proteins.
31029001	2	5	theme	extract	351:357	arg1	activities					333:342	The antioxidant, anti-inflammatory, analgesic, and antipyretic activities	270:342	The antioxidant, anti-inflammatory, analgesic, and antipyretic activities of the extract	270:357	The antioxidant, anti-inflammatory, analgesic, and antipyretic activities of the extract were investigated.
31029001	3	6	from	cavity	549:554	arg1	mice					559:562	mice	559:562	mice	559:562	In the carrageenan induced hind paw edema bioassay; the extract significantly reduced the edema thickness in rats and diminished the leukocyte migration to the peritoneal cavity in mice.
31029001	0	7	theme	antipyretic	92:102	arg1	activities					104:113	antipyretic activities	92:113	antipyretic activities	92:113	Albizia anthelmintica: HPLC-MS/MS profiling and in vivo anti-inflammatory, pain killing and antipyretic activities of its leaf extract.
31029001	9	8	theme	Albizia	1505:1511	arg1	antioxidant					1537:1547	a good antioxidant	1530:1547	a good antioxidant with potential therapeutic efficacy for treating inflammation, pain and related oxidative stress disorders	1530:1654	These findings suggested that Albizia anthelmintica is a good antioxidant with potential therapeutic efficacy for treating inflammation, pain and related oxidative stress disorders.
31029001	9	8	theme	Albizia	1505:1511	arg1	anthelmintica					1513:1525	Albizia anthelmintica	1505:1525	Albizia anthelmintica	1505:1525	These findings suggested that Albizia anthelmintica is a good antioxidant with potential therapeutic efficacy for treating inflammation, pain and related oxidative stress disorders.
31029001	3	9	theme	induced	397:403	arg1	bioassay					420:427	the carrageenan induced hind paw edema bioassay	381:427	the carrageenan induced hind paw edema bioassay	381:427	In the carrageenan induced hind paw edema bioassay; the extract significantly reduced the edema thickness in rats and diminished the leukocyte migration to the peritoneal cavity in mice.
31029001	2	10	theme	anti-inflammatory	287:303	arg1	activities					333:342	The antioxidant, anti-inflammatory, analgesic, and antipyretic activities	270:342	The antioxidant, anti-inflammatory, analgesic, and antipyretic activities of the extract	270:357	The antioxidant, anti-inflammatory, analgesic, and antipyretic activities of the extract were investigated.
31029001	8	11	theme	solid	1371:1375	arg1	properties					1389:1398	solid antioxidant properties	1371:1398	solid antioxidant properties	1371:1398	The extract displayed solid antioxidant properties as well with a TAC value of 35.13 U/L and EC50of 5.36 μg/mL in DPPH assay.
31029001	4	12	theme	peripheral	599:608	arg1	effects					627:633	central and peripheral anti-nociceptive effects	587:633	central and peripheral anti-nociceptive effects	587:633	The extract exhibited central and peripheral anti-nociceptive effects; it significantly decreased the number of acetic acid induced writhes and prolonged the latency time in the hot plate test.
31029001	9	13	theme	potential	1554:1562	arg1	efficacy					1576:1583	potential therapeutic efficacy	1554:1583	potential therapeutic efficacy for treating inflammation, pain and related oxidative stress disorders	1554:1654	These findings suggested that Albizia anthelmintica is a good antioxidant with potential therapeutic efficacy for treating inflammation, pain and related oxidative stress disorders.
31029001	7	14	dep	in	1198:1199	arg1	vitro					1201:1205	vitro	1201:1205	vitro	1201:1205	These findings were confirmed with in vitro enzyme inhibitory assays in which the extract showed IC50 values of 4.11, 0.054, and 1.74 μg/mL towards COX-1, COX-2 and 5-LOX, respectively.
31029001	7	15	theme	IC50	1260:1263	arg1	values					1265:1270	IC50 values	1260:1270	IC50 values of 4.11, 0.054, and 1.74 μg/mL towards COX-1, COX-2 and 5-LOX	1260:1332	These findings were confirmed with in vitro enzyme inhibitory assays in which the extract showed IC50 values of 4.11, 0.054, and 1.74 μg/mL towards COX-1, COX-2 and 5-LOX, respectively.
31029001	6	16	theme	conserved	1107:1115	arg1	residues					1128:1135	the conserved amino acid residues	1103:1135	the conserved amino acid residues in these target proteins	1103:1160	Molecular docking of some major compounds in the extract to COX-1, COX-2 and 5-LOX, enzymes involved in the inflammation cascade, revealed appreciable interactions with the conserved amino acid residues in these target proteins.
31029001	7	17	theme	0.054	1281:1285	arg1	values					1265:1270	IC50 values	1260:1270	IC50 values of 4.11, 0.054, and 1.74 μg/mL towards COX-1, COX-2 and 5-LOX	1260:1332	These findings were confirmed with in vitro enzyme inhibitory assays in which the extract showed IC50 values of 4.11, 0.054, and 1.74 μg/mL towards COX-1, COX-2 and 5-LOX, respectively.
31029001	2	18	theme	antioxidant	274:284	arg1	activities					333:342	The antioxidant, anti-inflammatory, analgesic, and antipyretic activities	270:342	The antioxidant, anti-inflammatory, analgesic, and antipyretic activities of the extract	270:357	The antioxidant, anti-inflammatory, analgesic, and antipyretic activities of the extract were investigated.
31029001	4	19	theme	induced	689:695	arg1	writhes					697:703	acetic acid induced writhes	677:703	acetic acid induced writhes	677:703	The extract exhibited central and peripheral anti-nociceptive effects; it significantly decreased the number of acetic acid induced writhes and prolonged the latency time in the hot plate test.
31029001	4	20	theme	acetic	677:682	arg1	acid					684:687	acetic acid	677:687	acetic acid induced writhes	677:703	The extract exhibited central and peripheral anti-nociceptive effects; it significantly decreased the number of acetic acid induced writhes and prolonged the latency time in the hot plate test.
31029001	7	21	theme	enzyme	1207:1212	arg1	assays					1225:1230	in vitro enzyme inhibitory assays	1198:1230	in vitro enzyme inhibitory assays in which the extract showed IC50 values of 4.11, 0.054, and 1.74 μg/mL towards COX-1, COX-2 and 5-LOX, respectively	1198:1346	These findings were confirmed with in vitro enzyme inhibitory assays in which the extract showed IC50 values of 4.11, 0.054, and 1.74 μg/mL towards COX-1, COX-2 and 5-LOX, respectively.
31029001	9	22	with	antioxidant	1537:1547	arg1	efficacy					1576:1583	potential therapeutic efficacy	1554:1583	potential therapeutic efficacy for treating inflammation, pain and related oxidative stress disorders	1554:1654	These findings suggested that Albizia anthelmintica is a good antioxidant with potential therapeutic efficacy for treating inflammation, pain and related oxidative stress disorders.
31029001	5	23	theme	substantial	780:790	arg1	activity					804:811	a substantial antipyretic activity	778:811	a substantial antipyretic activity	778:811	The extract showed a substantial antipyretic activity as it decreased significantly the elevated rectal temperature in mice after intraperitoneal injection of Brewer's yeast.
31029001	7	24	theme	inhibitory	1214:1223	arg1	assays					1225:1230	in vitro enzyme inhibitory assays	1198:1230	in vitro enzyme inhibitory assays in which the extract showed IC50 values of 4.11, 0.054, and 1.74 μg/mL towards COX-1, COX-2 and 5-LOX, respectively	1198:1346	These findings were confirmed with in vitro enzyme inhibitory assays in which the extract showed IC50 values of 4.11, 0.054, and 1.74 μg/mL towards COX-1, COX-2 and 5-LOX, respectively.
31029001	3	25	theme	peritoneal	538:547	arg1	cavity					549:554	the peritoneal cavity	534:554	the peritoneal cavity in mice	534:562	In the carrageenan induced hind paw edema bioassay; the extract significantly reduced the edema thickness in rats and diminished the leukocyte migration to the peritoneal cavity in mice.
31029001	3	26	theme	carrageenan	385:395	arg1	bioassay					420:427	the carrageenan induced hind paw edema bioassay	381:427	the carrageenan induced hind paw edema bioassay	381:427	In the carrageenan induced hind paw edema bioassay; the extract significantly reduced the edema thickness in rats and diminished the leukocyte migration to the peritoneal cavity in mice.
31029001	4	27	theme	plate	747:751	arg1	test					753:756	the hot plate test	739:756	the hot plate test	739:756	The extract exhibited central and peripheral anti-nociceptive effects; it significantly decreased the number of acetic acid induced writhes and prolonged the latency time in the hot plate test.
31029001	6	28	theme	appreciable	1073:1083	arg1	interactions					1085:1096	appreciable interactions	1073:1096	appreciable interactions with the conserved amino acid residues in these target proteins	1073:1160	Molecular docking of some major compounds in the extract to COX-1, COX-2 and 5-LOX, enzymes involved in the inflammation cascade, revealed appreciable interactions with the conserved amino acid residues in these target proteins.
31029001	1	29	theme	phytochemical	161:173	arg1	composition					175:185	the phytochemical composition	157:185	the phytochemical composition of a leaf methanol extract from Albizia anthelmintica	157:239	In the current work, the phytochemical composition of a leaf methanol extract from Albizia anthelmintica was thoroughly investigated.
31029001	0	30	theme	Albizia	0:6	arg1	anthelmintica					8:20	Albizia anthelmintica	0:20	Albizia anthelmintica: HPLC-MS/MS profiling and in vivo anti-inflammatory, pain killing and antipyretic activities of its leaf extract.	0:134	Albizia anthelmintica: HPLC-MS/MS profiling and in vivo anti-inflammatory, pain killing and antipyretic activities of its leaf extract.
31029001	8	31	theme	DPPH	1463:1466	arg1	assay					1468:1472	DPPH assay	1463:1472	DPPH assay	1463:1472	The extract displayed solid antioxidant properties as well with a TAC value of 35.13 U/L and EC50of 5.36 μg/mL in DPPH assay.
31029001	4	32	theme	hot	743:745	arg1	test					753:756	the hot plate test	739:756	the hot plate test	739:756	The extract exhibited central and peripheral anti-nociceptive effects; it significantly decreased the number of acetic acid induced writhes and prolonged the latency time in the hot plate test.
31029001	4	33	theme	acid	684:687	arg1	writhes					697:703	acetic acid induced writhes	677:703	acetic acid induced writhes	677:703	The extract exhibited central and peripheral anti-nociceptive effects; it significantly decreased the number of acetic acid induced writhes and prolonged the latency time in the hot plate test.
31029001	0	34	theme	HPLC-MS/MS	23:32	arg1	profiling					34:42	HPLC-MS/MS profiling	23:42	HPLC-MS/MS profiling	23:42	Albizia anthelmintica: HPLC-MS/MS profiling and in vivo anti-inflammatory, pain killing and antipyretic activities of its leaf extract.
31029001	9	35	theme	oxidative	1629:1637	arg1	stress					1639:1644	related oxidative stress	1621:1644	related oxidative stress	1621:1644	These findings suggested that Albizia anthelmintica is a good antioxidant with potential therapeutic efficacy for treating inflammation, pain and related oxidative stress disorders.
31029001	0	36	theme	leaf	122:125	arg1	extract					127:133	its leaf extract	118:133	its leaf extract	118:133	Albizia anthelmintica: HPLC-MS/MS profiling and in vivo anti-inflammatory, pain killing and antipyretic activities of its leaf extract.
31029001	2	37	theme	analgesic	306:314	arg1	activities					333:342	The antioxidant, anti-inflammatory, analgesic, and antipyretic activities	270:342	The antioxidant, anti-inflammatory, analgesic, and antipyretic activities of the extract	270:357	The antioxidant, anti-inflammatory, analgesic, and antipyretic activities of the extract were investigated.
31029001	3	38	theme	edema	468:472	arg1	thickness					474:482	the edema thickness	464:482	the edema thickness in rats	464:490	In the carrageenan induced hind paw edema bioassay; the extract significantly reduced the edema thickness in rats and diminished the leukocyte migration to the peritoneal cavity in mice.
31029001	9	39	theme	therapeutic	1564:1574	arg1	efficacy					1576:1583	potential therapeutic efficacy	1554:1583	potential therapeutic efficacy for treating inflammation, pain and related oxidative stress disorders	1554:1654	These findings suggested that Albizia anthelmintica is a good antioxidant with potential therapeutic efficacy for treating inflammation, pain and related oxidative stress disorders.
31029001	8	40	theme	U/L	1434:1436	arg1	value					1419:1423	a TAC value	1413:1423	a TAC value of 35.13 U/L and EC50of 5.36 μg/mL	1413:1458	The extract displayed solid antioxidant properties as well with a TAC value of 35.13 U/L and EC50of 5.36 μg/mL in DPPH assay.
31029001	3	41	theme	leukocyte	511:519	arg1	migration					521:529	the leukocyte migration	507:529	the leukocyte migration to the peritoneal cavity in mice	507:562	In the carrageenan induced hind paw edema bioassay; the extract significantly reduced the edema thickness in rats and diminished the leukocyte migration to the peritoneal cavity in mice.
31029001	5	42	theme	rectal	856:861	arg1	temperature					863:873	the elevated rectal temperature	843:873	the elevated rectal temperature in mice	843:881	The extract showed a substantial antipyretic activity as it decreased significantly the elevated rectal temperature in mice after intraperitoneal injection of Brewer's yeast.
31029001	3	43	theme	edema	414:418	arg1	bioassay					420:427	the carrageenan induced hind paw edema bioassay	381:427	the carrageenan induced hind paw edema bioassay	381:427	In the carrageenan induced hind paw edema bioassay; the extract significantly reduced the edema thickness in rats and diminished the leukocyte migration to the peritoneal cavity in mice.
31029001	7	44	theme	1.74 μg/mL	1292:1301	arg1	values					1265:1270	IC50 values	1260:1270	IC50 values of 4.11, 0.054, and 1.74 μg/mL towards COX-1, COX-2 and 5-LOX	1260:1332	These findings were confirmed with in vitro enzyme inhibitory assays in which the extract showed IC50 values of 4.11, 0.054, and 1.74 μg/mL towards COX-1, COX-2 and 5-LOX, respectively.
31029001	6	45	theme	inflammation	1042:1053	arg1	cascade					1055:1061	the inflammation cascade	1038:1061	the inflammation cascade	1038:1061	Molecular docking of some major compounds in the extract to COX-1, COX-2 and 5-LOX, enzymes involved in the inflammation cascade, revealed appreciable interactions with the conserved amino acid residues in these target proteins.
31029001	1	46	theme	leaf	192:195	arg1	extract					206:212	a leaf methanol extract	190:212	a leaf methanol extract from Albizia anthelmintica	190:239	In the current work, the phytochemical composition of a leaf methanol extract from Albizia anthelmintica was thoroughly investigated.
31029001	5	47	theme	antipyretic	792:802	arg1	activity					804:811	a substantial antipyretic activity	778:811	a substantial antipyretic activity	778:811	The extract showed a substantial antipyretic activity as it decreased significantly the elevated rectal temperature in mice after intraperitoneal injection of Brewer's yeast.
31029001	0	48	theme	extract	127:133	arg1	killing					80:86	killing	80:86	killing	80:86	Albizia anthelmintica: HPLC-MS/MS profiling and in vivo anti-inflammatory, pain killing and antipyretic activities of its leaf extract.
31029001	0	48	theme	extract	127:133	arg1	activities					104:113	antipyretic activities	92:113	antipyretic activities	92:113	Albizia anthelmintica: HPLC-MS/MS profiling and in vivo anti-inflammatory, pain killing and antipyretic activities of its leaf extract.
31029001	8	49	theme	EC50of	1442:1447	arg1	5.36 μg/mL					1449:1458	EC50of 5.36 μg/mL	1442:1458	EC50of 5.36 μg/mL	1442:1458	The extract displayed solid antioxidant properties as well with a TAC value of 35.13 U/L and EC50of 5.36 μg/mL in DPPH assay.
31029001	9	50	theme	inflammation	1598:1609	arg1	disorders					1646:1654	inflammation, pain and related oxidative stress disorders	1598:1654	inflammation, pain and related oxidative stress disorders	1598:1654	These findings suggested that Albizia anthelmintica is a good antioxidant with potential therapeutic efficacy for treating inflammation, pain and related oxidative stress disorders.
31029001	5	51	from	temperature	863:873	arg1	mice					878:881	mice	878:881	mice	878:881	The extract showed a substantial antipyretic activity as it decreased significantly the elevated rectal temperature in mice after intraperitoneal injection of Brewer's yeast.
31029001	1	52	theme	methanol	197:204	arg1	extract					206:212	a leaf methanol extract	190:212	a leaf methanol extract from Albizia anthelmintica	190:239	In the current work, the phytochemical composition of a leaf methanol extract from Albizia anthelmintica was thoroughly investigated.
31029001	3	53	theme	hind	405:408	arg1	bioassay					420:427	the carrageenan induced hind paw edema bioassay	381:427	the carrageenan induced hind paw edema bioassay	381:427	In the carrageenan induced hind paw edema bioassay; the extract significantly reduced the edema thickness in rats and diminished the leukocyte migration to the peritoneal cavity in mice.
31029001	5	54	theme	intraperitoneal	889:903	arg1	injection					905:913	intraperitoneal injection	889:913	intraperitoneal injection of Brewer's yeast	889:931	The extract showed a substantial antipyretic activity as it decreased significantly the elevated rectal temperature in mice after intraperitoneal injection of Brewer's yeast.
31029001	6	55	with	interactions	1085:1096	arg1	residues					1128:1135	the conserved amino acid residues	1103:1135	the conserved amino acid residues in these target proteins	1103:1160	Molecular docking of some major compounds in the extract to COX-1, COX-2 and 5-LOX, enzymes involved in the inflammation cascade, revealed appreciable interactions with the conserved amino acid residues in these target proteins.
31029001	6	56	from	residues	1128:1135	arg1	proteins					1153:1160	these target proteins	1140:1160	these target proteins	1140:1160	Molecular docking of some major compounds in the extract to COX-1, COX-2 and 5-LOX, enzymes involved in the inflammation cascade, revealed appreciable interactions with the conserved amino acid residues in these target proteins.
31029001	6	57	theme	target	1146:1151	arg1	proteins					1153:1160	these target proteins	1140:1160	these target proteins	1140:1160	Molecular docking of some major compounds in the extract to COX-1, COX-2 and 5-LOX, enzymes involved in the inflammation cascade, revealed appreciable interactions with the conserved amino acid residues in these target proteins.
31029001	3	58	theme	paw	410:412	arg1	bioassay					420:427	the carrageenan induced hind paw edema bioassay	381:427	the carrageenan induced hind paw edema bioassay	381:427	In the carrageenan induced hind paw edema bioassay; the extract significantly reduced the edema thickness in rats and diminished the leukocyte migration to the peritoneal cavity in mice.
31029001	6	59	theme	major	960:964	arg1	compounds					966:974	some major compounds	955:974	some major compounds in the extract	955:989	Molecular docking of some major compounds in the extract to COX-1, COX-2 and 5-LOX, enzymes involved in the inflammation cascade, revealed appreciable interactions with the conserved amino acid residues in these target proteins.
31029001	1	60	from	anthelmintica	227:239	arg1	extract					206:212	a leaf methanol extract	190:212	a leaf methanol extract from Albizia anthelmintica	190:239	In the current work, the phytochemical composition of a leaf methanol extract from Albizia anthelmintica was thoroughly investigated.
31029001	1	60	from	anthelmintica	227:239	arg1	composition					175:185	the phytochemical composition	157:185	the phytochemical composition of a leaf methanol extract from Albizia anthelmintica	157:239	In the current work, the phytochemical composition of a leaf methanol extract from Albizia anthelmintica was thoroughly investigated.
31029001	9	61	theme	pain	1612:1615	arg1	disorders					1646:1654	inflammation, pain and related oxidative stress disorders	1598:1654	inflammation, pain and related oxidative stress disorders	1598:1654	These findings suggested that Albizia anthelmintica is a good antioxidant with potential therapeutic efficacy for treating inflammation, pain and related oxidative stress disorders.
31029001	4	62	theme	latency	723:729	arg1	time					731:734	the latency time	719:734	the latency time in the hot plate test	719:756	The extract exhibited central and peripheral anti-nociceptive effects; it significantly decreased the number of acetic acid induced writhes and prolonged the latency time in the hot plate test.
31029001	9	63	theme	good	1532:1535	arg1	antioxidant					1537:1547	a good antioxidant	1530:1547	a good antioxidant with potential therapeutic efficacy for treating inflammation, pain and related oxidative stress disorders	1530:1654	These findings suggested that Albizia anthelmintica is a good antioxidant with potential therapeutic efficacy for treating inflammation, pain and related oxidative stress disorders.
31029001	9	63	theme	good	1532:1535	arg1	anthelmintica					1513:1525	Albizia anthelmintica	1505:1525	Albizia anthelmintica	1505:1525	These findings suggested that Albizia anthelmintica is a good antioxidant with potential therapeutic efficacy for treating inflammation, pain and related oxidative stress disorders.
31029001	8	64	theme	5.36 μg/mL	1449:1458	arg1	value					1419:1423	a TAC value	1413:1423	a TAC value of 35.13 U/L and EC50of 5.36 μg/mL	1413:1458	The extract displayed solid antioxidant properties as well with a TAC value of 35.13 U/L and EC50of 5.36 μg/mL in DPPH assay.
31029001	8	65	theme	TAC	1415:1417	arg1	value					1419:1423	a TAC value	1413:1423	a TAC value of 35.13 U/L and EC50of 5.36 μg/mL	1413:1458	The extract displayed solid antioxidant properties as well with a TAC value of 35.13 U/L and EC50of 5.36 μg/mL in DPPH assay.
31029001	3	66	from	thickness	474:482	arg1	rats					487:490	rats	487:490	rats	487:490	In the carrageenan induced hind paw edema bioassay; the extract significantly reduced the edema thickness in rats and diminished the leukocyte migration to the peritoneal cavity in mice.
31029001	7	67	theme	4.11	1275:1278	arg1	values					1265:1270	IC50 values	1260:1270	IC50 values of 4.11, 0.054, and 1.74 μg/mL towards COX-1, COX-2 and 5-LOX	1260:1332	These findings were confirmed with in vitro enzyme inhibitory assays in which the extract showed IC50 values of 4.11, 0.054, and 1.74 μg/mL towards COX-1, COX-2 and 5-LOX, respectively.
31029001	8	68	theme	antioxidant	1377:1387	arg1	properties					1389:1398	solid antioxidant properties	1371:1398	solid antioxidant properties	1371:1398	The extract displayed solid antioxidant properties as well with a TAC value of 35.13 U/L and EC50of 5.36 μg/mL in DPPH assay.
31029001	1	69	theme	extract	206:212	arg1	composition					175:185	the phytochemical composition	157:185	the phytochemical composition of a leaf methanol extract from Albizia anthelmintica	157:239	In the current work, the phytochemical composition of a leaf methanol extract from Albizia anthelmintica was thoroughly investigated.
31029001	6	70	from	compounds	966:974	arg1	extract					983:989	the extract	979:989	the extract	979:989	Molecular docking of some major compounds in the extract to COX-1, COX-2 and 5-LOX, enzymes involved in the inflammation cascade, revealed appreciable interactions with the conserved amino acid residues in these target proteins.
31029001	9	71	theme	related	1621:1627	arg1	stress					1639:1644	related oxidative stress	1621:1644	related oxidative stress	1621:1644	These findings suggested that Albizia anthelmintica is a good antioxidant with potential therapeutic efficacy for treating inflammation, pain and related oxidative stress disorders.
31029001	2	72	theme	antipyretic	321:331	arg1	activities					333:342	The antioxidant, anti-inflammatory, analgesic, and antipyretic activities	270:342	The antioxidant, anti-inflammatory, analgesic, and antipyretic activities of the extract	270:357	The antioxidant, anti-inflammatory, analgesic, and antipyretic activities of the extract were investigated.
31029001	4	73	from	time	731:734	arg1	test					753:756	the hot plate test	739:756	the hot plate test	739:756	The extract exhibited central and peripheral anti-nociceptive effects; it significantly decreased the number of acetic acid induced writhes and prolonged the latency time in the hot plate test.
31029001	4	74	theme	anti-nociceptive	610:625	arg1	effects					627:633	central and peripheral anti-nociceptive effects	587:633	central and peripheral anti-nociceptive effects	587:633	The extract exhibited central and peripheral anti-nociceptive effects; it significantly decreased the number of acetic acid induced writhes and prolonged the latency time in the hot plate test.
31029001	6	75	theme	amino	1117:1121	arg1	residues					1128:1135	the conserved amino acid residues	1103:1135	the conserved amino acid residues in these target proteins	1103:1160	Molecular docking of some major compounds in the extract to COX-1, COX-2 and 5-LOX, enzymes involved in the inflammation cascade, revealed appreciable interactions with the conserved amino acid residues in these target proteins.
31029001	0	76	dep	anthelmintica	8:20	arg1	pain					75:78	pain	75:78	pain killing and antipyretic activities of its leaf extract	75:133	Albizia anthelmintica: HPLC-MS/MS profiling and in vivo anti-inflammatory, pain killing and antipyretic activities of its leaf extract.
31029001	1	77	from	composition	175:185	arg1	anthelmintica					227:239	Albizia anthelmintica	219:239	Albizia anthelmintica	219:239	In the current work, the phytochemical composition of a leaf methanol extract from Albizia anthelmintica was thoroughly investigated.
31029001	6	78	theme	acid	1123:1126	arg1	residues					1128:1135	the conserved amino acid residues	1103:1135	the conserved amino acid residues in these target proteins	1103:1160	Molecular docking of some major compounds in the extract to COX-1, COX-2 and 5-LOX, enzymes involved in the inflammation cascade, revealed appreciable interactions with the conserved amino acid residues in these target proteins.
31029001	6	79	from	extract	983:989	arg1	docking					944:950	Molecular docking	934:950	Molecular docking of some major compounds in the extract to COX-1, COX-2 and 5-LOX, enzymes involved in the inflammation cascade,	934:1062	Molecular docking of some major compounds in the extract to COX-1, COX-2 and 5-LOX, enzymes involved in the inflammation cascade, revealed appreciable interactions with the conserved amino acid residues in these target proteins.
31029001	1	80	theme	Albizia	219:225	arg1	anthelmintica					227:239	Albizia anthelmintica	219:239	Albizia anthelmintica	219:239	In the current work, the phytochemical composition of a leaf methanol extract from Albizia anthelmintica was thoroughly investigated.
31029001	9	81	theme	stress	1639:1644	arg1	disorders					1646:1654	inflammation, pain and related oxidative stress disorders	1598:1654	inflammation, pain and related oxidative stress disorders	1598:1654	These findings suggested that Albizia anthelmintica is a good antioxidant with potential therapeutic efficacy for treating inflammation, pain and related oxidative stress disorders.
31029001	4	82	theme	writhes	697:703	arg1	number					667:672	the number	663:672	the number of acetic acid induced writhes	663:703	The extract exhibited central and peripheral anti-nociceptive effects; it significantly decreased the number of acetic acid induced writhes and prolonged the latency time in the hot plate test.
31029001	7	83	theme	in	1198:1199	arg1	assays					1225:1230	in vitro enzyme inhibitory assays	1198:1230	in vitro enzyme inhibitory assays in which the extract showed IC50 values of 4.11, 0.054, and 1.74 μg/mL towards COX-1, COX-2 and 5-LOX, respectively	1198:1346	These findings were confirmed with in vitro enzyme inhibitory assays in which the extract showed IC50 values of 4.11, 0.054, and 1.74 μg/mL towards COX-1, COX-2 and 5-LOX, respectively.
31029001	6	84	theme	compounds	966:974	arg1	docking					944:950	Molecular docking	934:950	Molecular docking of some major compounds in the extract to COX-1, COX-2 and 5-LOX, enzymes involved in the inflammation cascade,	934:1062	Molecular docking of some major compounds in the extract to COX-1, COX-2 and 5-LOX, enzymes involved in the inflammation cascade, revealed appreciable interactions with the conserved amino acid residues in these target proteins.
30470954	9	0	theme	collagen	1578:1585	arg1	deposition					1587:1596	collagen deposition	1578:1596	collagen deposition	1578:1596	RESULTS Exposure to LPS (0.1 µg) exacerbates airway inflammation and induces structural changes in lungs by enhanced ROS production, collagen deposition, expression of genes involved in airway remodeling and activation of MAP kinases pathway enzymes.
30470954	3	1	theme	OVA	674:676	arg1	exposure					690:697	OVA (ovalbumin) exposure	674:697	OVA (ovalbumin) exposure	674:697	Curcumin (diferuloylmethane), well known for its anti-inflammatory potential, was administered through intranasal route 1 h before LPS and OVA (ovalbumin) exposure to evaluate its efficacy against airway structural changes.
30470954	11	2	theme	intranasal	1858:1867	arg1	curcumin					1869:1876	intranasal curcumin	1858:1876	intranasal curcumin	1858:1876	CONCLUSION Our results strongly suggest that intranasal curcumin effectively protects LPS-induced airway inflammation and structural changes by modulating genes involved in airway remodeling in safer way; hence, it can be considered as supplementary alternative towards asthma treatments.
30470954	1	3	theme	lipopolysaccharide	283:300	arg1	component					335:343	the outermost component	321:343	the outermost component of cell wall	321:356	OBJECTIVE Bacterial infections can exacerbate asthmatic inflammation depending on lipopolysaccharide (LPS) composition, the outermost component of cell wall, its exposure timings as well as host's immune status.
30470954	1	3	theme	lipopolysaccharide	283:300	arg1	status					405:410	host's immune status	391:410	its exposure timings as well as host's immune status	359:410	OBJECTIVE Bacterial infections can exacerbate asthmatic inflammation depending on lipopolysaccharide (LPS) composition, the outermost component of cell wall, its exposure timings as well as host's immune status.
30470954	1	3	theme	lipopolysaccharide	283:300	arg1	composition					308:318	lipopolysaccharide (LPS) composition	283:318	lipopolysaccharide (LPS) composition	283:318	OBJECTIVE Bacterial infections can exacerbate asthmatic inflammation depending on lipopolysaccharide (LPS) composition, the outermost component of cell wall, its exposure timings as well as host's immune status.
30470954	1	3	theme	lipopolysaccharide	283:300	arg1	timings					372:378	its exposure timings	359:378	its exposure timings as well as host's immune status	359:410	OBJECTIVE Bacterial infections can exacerbate asthmatic inflammation depending on lipopolysaccharide (LPS) composition, the outermost component of cell wall, its exposure timings as well as host's immune status.
30470954	6	4	theme	IL-13	1166:1170	arg1	expressions					1128:1138	mRNA expressions	1123:1138	mRNA expressions of MMP-9, TIMP-1, TGF-β1, IL-13, Collagen-1 and TLR-4	1123:1192	MMP-9 activity was evaluated by gelatin zymography and mRNA expressions of MMP-9, TIMP-1, TGF-β1, IL-13, Collagen-1 and TLR-4 were measured in lungs.
30470954	10	5	theme	Intranasal	1696:1705	arg1	pretreatment					1716:1727	Intranasal curcumin pretreatment	1696:1727	Intranasal curcumin pretreatment	1696:1727	Intranasal curcumin pretreatment had significantly suppressed inflammatory mediators and airway remodeling proteins.
30470954	6	6	theme	TGF-β1	1158:1163	arg1	expressions					1128:1138	mRNA expressions	1123:1138	mRNA expressions of MMP-9, TIMP-1, TGF-β1, IL-13, Collagen-1 and TLR-4	1123:1192	MMP-9 activity was evaluated by gelatin zymography and mRNA expressions of MMP-9, TIMP-1, TGF-β1, IL-13, Collagen-1 and TLR-4 were measured in lungs.
30470954	6	7	theme	TIMP-1	1150:1155	arg1	expressions					1128:1138	mRNA expressions	1123:1138	mRNA expressions of MMP-9, TIMP-1, TGF-β1, IL-13, Collagen-1 and TLR-4	1123:1192	MMP-9 activity was evaluated by gelatin zymography and mRNA expressions of MMP-9, TIMP-1, TGF-β1, IL-13, Collagen-1 and TLR-4 were measured in lungs.
30470954	9	8	theme	MAP	1667:1669	arg1	kinases					1671:1677	MAP kinases	1667:1677	MAP kinases	1667:1677	RESULTS Exposure to LPS (0.1 µg) exacerbates airway inflammation and induces structural changes in lungs by enhanced ROS production, collagen deposition, expression of genes involved in airway remodeling and activation of MAP kinases pathway enzymes.
30470954	3	9	theme	airway	732:737	arg1	changes					750:756	airway structural changes	732:756	airway structural changes	732:756	Curcumin (diferuloylmethane), well known for its anti-inflammatory potential, was administered through intranasal route 1 h before LPS and OVA (ovalbumin) exposure to evaluate its efficacy against airway structural changes.
30470954	9	10	theme	airway	1631:1636	arg1	remodeling					1638:1647	airway remodeling	1631:1647	airway remodeling	1631:1647	RESULTS Exposure to LPS (0.1 µg) exacerbates airway inflammation and induces structural changes in lungs by enhanced ROS production, collagen deposition, expression of genes involved in airway remodeling and activation of MAP kinases pathway enzymes.
30470954	6	11	theme	MMP-9	1143:1147	arg1	expressions					1128:1138	mRNA expressions	1123:1138	mRNA expressions of MMP-9, TIMP-1, TGF-β1, IL-13, Collagen-1 and TLR-4	1123:1192	MMP-9 activity was evaluated by gelatin zymography and mRNA expressions of MMP-9, TIMP-1, TGF-β1, IL-13, Collagen-1 and TLR-4 were measured in lungs.
30470954	9	12	dep	production	1566:1575	arg1	enzymes					1687:1693	pathway enzymes	1679:1693	pathway enzymes	1679:1693	RESULTS Exposure to LPS (0.1 µg) exacerbates airway inflammation and induces structural changes in lungs by enhanced ROS production, collagen deposition, expression of genes involved in airway remodeling and activation of MAP kinases pathway enzymes.
30470954	1	13	theme	Bacterial	211:219	arg1	infections					221:230	OBJECTIVE Bacterial infections	201:230	OBJECTIVE Bacterial infections	201:230	OBJECTIVE Bacterial infections can exacerbate asthmatic inflammation depending on lipopolysaccharide (LPS) composition, the outermost component of cell wall, its exposure timings as well as host's immune status.
30470954	8	14	theme	trichrome	1375:1383	arg1	staining					1385:1392	masson's trichrome staining	1366:1392	masson's trichrome staining	1366:1392	Hydroxyproline level and masson's trichrome staining were used to evaluate collagen deposition in lung.
30470954	1	15	theme	immune	398:403	arg1	status					405:410	host's immune status	391:410	its exposure timings as well as host's immune status	359:410	OBJECTIVE Bacterial infections can exacerbate asthmatic inflammation depending on lipopolysaccharide (LPS) composition, the outermost component of cell wall, its exposure timings as well as host's immune status.
30470954	1	15	theme	immune	398:403	arg1	composition					308:318	lipopolysaccharide (LPS) composition	283:318	lipopolysaccharide (LPS) composition	283:318	OBJECTIVE Bacterial infections can exacerbate asthmatic inflammation depending on lipopolysaccharide (LPS) composition, the outermost component of cell wall, its exposure timings as well as host's immune status.
30470954	4	16	theme	major	922:926	arg1	protein					934:940	major basic protein	922:940	major basic protein (MBP)	922:946	METHODS Inflammatory cell infiltration in lungs was measured by flow cytometry and further eosinophils were especially measured by immunofluorescence detection of major basic protein (MBP) as marker of eosinophilc granule protein.
30470954	4	16	theme	major	922:926	arg1	MBP					943:945	MBP	943:945	MBP	943:945	METHODS Inflammatory cell infiltration in lungs was measured by flow cytometry and further eosinophils were especially measured by immunofluorescence detection of major basic protein (MBP) as marker of eosinophilc granule protein.
30470954	10	17	theme	inflammatory	1758:1769	arg1	mediators					1771:1779	inflammatory mediators	1758:1779	inflammatory mediators	1758:1779	Intranasal curcumin pretreatment had significantly suppressed inflammatory mediators and airway remodeling proteins.
30470954	9	18	theme	kinases	1671:1677	arg1	expression					1599:1608	expression	1599:1608	expression of genes involved in airway remodeling	1599:1647	RESULTS Exposure to LPS (0.1 µg) exacerbates airway inflammation and induces structural changes in lungs by enhanced ROS production, collagen deposition, expression of genes involved in airway remodeling and activation of MAP kinases pathway enzymes.
30470954	9	18	theme	kinases	1671:1677	arg1	production					1566:1575	enhanced ROS production	1553:1575	enhanced ROS production	1553:1575	RESULTS Exposure to LPS (0.1 µg) exacerbates airway inflammation and induces structural changes in lungs by enhanced ROS production, collagen deposition, expression of genes involved in airway remodeling and activation of MAP kinases pathway enzymes.
30470954	9	18	theme	kinases	1671:1677	arg1	deposition					1587:1596	collagen deposition	1578:1596	collagen deposition	1578:1596	RESULTS Exposure to LPS (0.1 µg) exacerbates airway inflammation and induces structural changes in lungs by enhanced ROS production, collagen deposition, expression of genes involved in airway remodeling and activation of MAP kinases pathway enzymes.
30470954	9	18	theme	kinases	1671:1677	arg1	activation					1653:1662	activation	1653:1662	activation of MAP kinases	1653:1677	RESULTS Exposure to LPS (0.1 µg) exacerbates airway inflammation and induces structural changes in lungs by enhanced ROS production, collagen deposition, expression of genes involved in airway remodeling and activation of MAP kinases pathway enzymes.
30470954	6	19	theme	TLR-4	1188:1192	arg1	expressions					1128:1138	mRNA expressions	1123:1138	mRNA expressions of MMP-9, TIMP-1, TGF-β1, IL-13, Collagen-1 and TLR-4	1123:1192	MMP-9 activity was evaluated by gelatin zymography and mRNA expressions of MMP-9, TIMP-1, TGF-β1, IL-13, Collagen-1 and TLR-4 were measured in lungs.
30470954	0	20	theme	MAP	173:175	arg1	kinases					177:183	MAP kinases	173:183	MAP kinases	173:183	Intranasal curcumin protects against LPS-induced airway remodeling by modulating toll-like receptor-4 (TLR-4) and matrixmetalloproteinase-9 (MMP-9) expression via affecting MAP kinases in mouse model.
30470954	11	21	theme	safer	2007:2011	arg1	way					2013:2015	safer way	2007:2015	safer way	2007:2015	CONCLUSION Our results strongly suggest that intranasal curcumin effectively protects LPS-induced airway inflammation and structural changes by modulating genes involved in airway remodeling in safer way; hence, it can be considered as supplementary alternative towards asthma treatments.
30470954	9	22	theme	RESULTS	1445:1451	arg1	Exposure					1453:1460	RESULTS Exposure	1445:1460	RESULTS Exposure to LPS (0.1 µg)	1445:1476	RESULTS Exposure to LPS (0.1 µg) exacerbates airway inflammation and induces structural changes in lungs by enhanced ROS production, collagen deposition, expression of genes involved in airway remodeling and activation of MAP kinases pathway enzymes.
30470954	11	23	theme	asthma	2083:2088	arg1	treatments					2090:2099	asthma treatments	2083:2099	asthma treatments	2083:2099	CONCLUSION Our results strongly suggest that intranasal curcumin effectively protects LPS-induced airway inflammation and structural changes by modulating genes involved in airway remodeling in safer way; hence, it can be considered as supplementary alternative towards asthma treatments.
30470954	4	24	theme	granule	973:979	arg1	protein					981:987	eosinophilc granule protein	961:987	eosinophilc granule protein	961:987	METHODS Inflammatory cell infiltration in lungs was measured by flow cytometry and further eosinophils were especially measured by immunofluorescence detection of major basic protein (MBP) as marker of eosinophilc granule protein.
30470954	8	25	theme	collagen	1416:1423	arg1	deposition					1425:1434	collagen deposition	1416:1434	collagen deposition in lung	1416:1442	Hydroxyproline level and masson's trichrome staining were used to evaluate collagen deposition in lung.
30470954	6	26	theme	Collagen-1	1173:1182	arg1	expressions					1128:1138	mRNA expressions	1123:1138	mRNA expressions of MMP-9, TIMP-1, TGF-β1, IL-13, Collagen-1 and TLR-4	1123:1192	MMP-9 activity was evaluated by gelatin zymography and mRNA expressions of MMP-9, TIMP-1, TGF-β1, IL-13, Collagen-1 and TLR-4 were measured in lungs.
30470954	1	27	theme	wall	353:356	arg1	component					335:343	the outermost component	321:343	the outermost component of cell wall	321:356	OBJECTIVE Bacterial infections can exacerbate asthmatic inflammation depending on lipopolysaccharide (LPS) composition, the outermost component of cell wall, its exposure timings as well as host's immune status.
30470954	1	27	theme	wall	353:356	arg1	composition					308:318	lipopolysaccharide (LPS) composition	283:318	lipopolysaccharide (LPS) composition	283:318	OBJECTIVE Bacterial infections can exacerbate asthmatic inflammation depending on lipopolysaccharide (LPS) composition, the outermost component of cell wall, its exposure timings as well as host's immune status.
30470954	10	28	theme	remodeling	1792:1801	arg1	proteins					1803:1810	airway remodeling proteins	1785:1810	airway remodeling proteins	1785:1810	Intranasal curcumin pretreatment had significantly suppressed inflammatory mediators and airway remodeling proteins.
30470954	4	29	from	infiltration	785:796	arg1	lungs					801:805	lungs	801:805	lungs	801:805	METHODS Inflammatory cell infiltration in lungs was measured by flow cytometry and further eosinophils were especially measured by immunofluorescence detection of major basic protein (MBP) as marker of eosinophilc granule protein.
30470954	4	30	dep	METHODS	759:765	arg1	measured					811:818	measured	811:818	was measured by flow cytometry and further eosinophils	807:860	METHODS Inflammatory cell infiltration in lungs was measured by flow cytometry and further eosinophils were especially measured by immunofluorescence detection of major basic protein (MBP) as marker of eosinophilc granule protein.
30470954	7	31	dep	kinases	1244:1250	arg1	P-p38					1267:1271	P-p38	1267:1271	P-p38	1267:1271	Protein expression of MAP kinases (P-ERK, P-JNK, P-p38), TLR-4, Cox-2, Lox-5 and Eotaxin was measured by western blotting.
30470954	7	31	dep	kinases	1244:1250	arg1	P-JNK					1260:1264	P-JNK	1260:1264	P-JNK	1260:1264	Protein expression of MAP kinases (P-ERK, P-JNK, P-p38), TLR-4, Cox-2, Lox-5 and Eotaxin was measured by western blotting.
30470954	7	31	dep	kinases	1244:1250	arg1	P-ERK					1253:1257	P-ERK	1253:1257	P-ERK	1253:1257	Protein expression of MAP kinases (P-ERK, P-JNK, P-p38), TLR-4, Cox-2, Lox-5 and Eotaxin was measured by western blotting.
30470954	4	32	theme	Inflammatory	767:778	arg1	infiltration					785:796	Inflammatory cell infiltration	767:796	Inflammatory cell infiltration in lungs	767:805	METHODS Inflammatory cell infiltration in lungs was measured by flow cytometry and further eosinophils were especially measured by immunofluorescence detection of major basic protein (MBP) as marker of eosinophilc granule protein.
30470954	11	33	theme	airway	1911:1916	arg1	inflammation					1918:1929	LPS-induced airway inflammation	1899:1929	LPS-induced airway inflammation	1899:1929	CONCLUSION Our results strongly suggest that intranasal curcumin effectively protects LPS-induced airway inflammation and structural changes by modulating genes involved in airway remodeling in safer way; hence, it can be considered as supplementary alternative towards asthma treatments.
30470954	9	34	theme	airway	1490:1495	arg1	inflammation					1497:1508	airway inflammation	1490:1508	airway inflammation	1490:1508	RESULTS Exposure to LPS (0.1 µg) exacerbates airway inflammation and induces structural changes in lungs by enhanced ROS production, collagen deposition, expression of genes involved in airway remodeling and activation of MAP kinases pathway enzymes.
30470954	6	35	theme	MMP-9	1068:1072	arg1	activity					1074:1081	MMP-9 activity	1068:1081	MMP-9 activity	1068:1081	MMP-9 activity was evaluated by gelatin zymography and mRNA expressions of MMP-9, TIMP-1, TGF-β1, IL-13, Collagen-1 and TLR-4 were measured in lungs.
30470954	0	36	theme	Intranasal	0:9	arg1	curcumin					11:18	Intranasal curcumin	0:18	Intranasal curcumin	0:18	Intranasal curcumin protects against LPS-induced airway remodeling by modulating toll-like receptor-4 (TLR-4) and matrixmetalloproteinase-9 (MMP-9) expression via affecting MAP kinases in mouse model.
30470954	2	37	theme	Balb/c	431:436	arg1	mice					438:441	Balb/c mice	431:441	Balb/c mice	431:441	In present study, Balb/c mice were exposed to antigen (ovalbumin) and LPS simultaneously to establish an asthmatic model.
30470954	3	38	theme	anti-inflammatory	584:600	arg1	potential					602:610	its anti-inflammatory potential	580:610	its anti-inflammatory potential	580:610	Curcumin (diferuloylmethane), well known for its anti-inflammatory potential, was administered through intranasal route 1 h before LPS and OVA (ovalbumin) exposure to evaluate its efficacy against airway structural changes.
30470954	9	39	theme	structural	1522:1531	arg1	changes					1533:1539	structural changes	1522:1539	structural changes in lungs by enhanced ROS production, collagen deposition, expression of genes involved in airway remodeling and activation of MAP kinases pathway enzymes	1522:1693	RESULTS Exposure to LPS (0.1 µg) exacerbates airway inflammation and induces structural changes in lungs by enhanced ROS production, collagen deposition, expression of genes involved in airway remodeling and activation of MAP kinases pathway enzymes.
30470954	9	40	from	changes	1533:1539	arg1	lungs					1544:1548	lungs	1544:1548	lungs by enhanced ROS production, collagen deposition, expression of genes involved in airway remodeling and activation of MAP kinases pathway enzymes	1544:1693	RESULTS Exposure to LPS (0.1 µg) exacerbates airway inflammation and induces structural changes in lungs by enhanced ROS production, collagen deposition, expression of genes involved in airway remodeling and activation of MAP kinases pathway enzymes.
30470954	3	41	theme	route	649:653	arg1	1 h					655:657	intranasal route 1 h	638:657	intranasal route 1 h before LPS and OVA (ovalbumin) exposure	638:697	Curcumin (diferuloylmethane), well known for its anti-inflammatory potential, was administered through intranasal route 1 h before LPS and OVA (ovalbumin) exposure to evaluate its efficacy against airway structural changes.
30470954	0	42	theme	LPS-induced	37:47	arg1	remodeling					56:65	LPS-induced airway remodeling	37:65	LPS-induced airway remodeling	37:65	Intranasal curcumin protects against LPS-induced airway remodeling by modulating toll-like receptor-4 (TLR-4) and matrixmetalloproteinase-9 (MMP-9) expression via affecting MAP kinases in mouse model.
30470954	1	43	theme	outermost	325:333	arg1	component					335:343	the outermost component	321:343	the outermost component of cell wall	321:356	OBJECTIVE Bacterial infections can exacerbate asthmatic inflammation depending on lipopolysaccharide (LPS) composition, the outermost component of cell wall, its exposure timings as well as host's immune status.
30470954	1	43	theme	outermost	325:333	arg1	composition					308:318	lipopolysaccharide (LPS) composition	283:318	lipopolysaccharide (LPS) composition	283:318	OBJECTIVE Bacterial infections can exacerbate asthmatic inflammation depending on lipopolysaccharide (LPS) composition, the outermost component of cell wall, its exposure timings as well as host's immune status.
30470954	8	44	theme	Hydroxyproline	1341:1354	arg1	level					1356:1360	Hydroxyproline level	1341:1360	Hydroxyproline level	1341:1360	Hydroxyproline level and masson's trichrome staining were used to evaluate collagen deposition in lung.
30470954	6	45	theme	mRNA	1123:1126	arg1	expressions					1128:1138	mRNA expressions	1123:1138	mRNA expressions of MMP-9, TIMP-1, TGF-β1, IL-13, Collagen-1 and TLR-4	1123:1192	MMP-9 activity was evaluated by gelatin zymography and mRNA expressions of MMP-9, TIMP-1, TGF-β1, IL-13, Collagen-1 and TLR-4 were measured in lungs.
30470954	9	46	theme	ROS	1562:1564	arg1	production					1566:1575	enhanced ROS production	1553:1575	enhanced ROS production	1553:1575	RESULTS Exposure to LPS (0.1 µg) exacerbates airway inflammation and induces structural changes in lungs by enhanced ROS production, collagen deposition, expression of genes involved in airway remodeling and activation of MAP kinases pathway enzymes.
30470954	7	47	theme	kinases	1244:1250	arg1	expression					1226:1235	Protein expression	1218:1235	Protein expression of MAP kinases (P-ERK, P-JNK, P-p38), TLR-4, Cox-2, Lox-5 and Eotaxin	1218:1305	Protein expression of MAP kinases (P-ERK, P-JNK, P-p38), TLR-4, Cox-2, Lox-5 and Eotaxin was measured by western blotting.
30470954	5	48	theme	reactive	1007:1014	arg1	species					1023:1029	reactive oxygen species	1007:1029	reactive oxygen species (ROS)	1007:1035	We also measured reactive oxygen species (ROS) in BALF by spectrofluorometry.
30470954	5	48	theme	reactive	1007:1014	arg1	ROS					1032:1034	ROS	1032:1034	ROS	1032:1034	We also measured reactive oxygen species (ROS) in BALF by spectrofluorometry.
30470954	0	49	theme	mouse	188:192	arg1	model					194:198	mouse model	188:198	mouse model	188:198	Intranasal curcumin protects against LPS-induced airway remodeling by modulating toll-like receptor-4 (TLR-4) and matrixmetalloproteinase-9 (MMP-9) expression via affecting MAP kinases in mouse model.
30470954	7	50	theme	Protein	1218:1224	arg1	expression					1226:1235	Protein expression	1218:1235	Protein expression of MAP kinases (P-ERK, P-JNK, P-p38), TLR-4, Cox-2, Lox-5 and Eotaxin	1218:1305	Protein expression of MAP kinases (P-ERK, P-JNK, P-p38), TLR-4, Cox-2, Lox-5 and Eotaxin was measured by western blotting.
30470954	4	51	theme	flow	823:826	arg1	cytometry					828:836	flow cytometry	823:836	flow cytometry	823:836	METHODS Inflammatory cell infiltration in lungs was measured by flow cytometry and further eosinophils were especially measured by immunofluorescence detection of major basic protein (MBP) as marker of eosinophilc granule protein.
30470954	10	52	theme	curcumin	1707:1714	arg1	pretreatment					1716:1727	Intranasal curcumin pretreatment	1696:1727	Intranasal curcumin pretreatment	1696:1727	Intranasal curcumin pretreatment had significantly suppressed inflammatory mediators and airway remodeling proteins.
30470954	9	53	theme	genes	1613:1617	arg1	expression					1599:1608	expression	1599:1608	expression of genes involved in airway remodeling	1599:1647	RESULTS Exposure to LPS (0.1 µg) exacerbates airway inflammation and induces structural changes in lungs by enhanced ROS production, collagen deposition, expression of genes involved in airway remodeling and activation of MAP kinases pathway enzymes.
30470954	9	53	theme	genes	1613:1617	arg1	production					1566:1575	enhanced ROS production	1553:1575	enhanced ROS production	1553:1575	RESULTS Exposure to LPS (0.1 µg) exacerbates airway inflammation and induces structural changes in lungs by enhanced ROS production, collagen deposition, expression of genes involved in airway remodeling and activation of MAP kinases pathway enzymes.
30470954	9	53	theme	genes	1613:1617	arg1	deposition					1587:1596	collagen deposition	1578:1596	collagen deposition	1578:1596	RESULTS Exposure to LPS (0.1 µg) exacerbates airway inflammation and induces structural changes in lungs by enhanced ROS production, collagen deposition, expression of genes involved in airway remodeling and activation of MAP kinases pathway enzymes.
30470954	9	53	theme	genes	1613:1617	arg1	activation					1653:1662	activation	1653:1662	activation of MAP kinases	1653:1677	RESULTS Exposure to LPS (0.1 µg) exacerbates airway inflammation and induces structural changes in lungs by enhanced ROS production, collagen deposition, expression of genes involved in airway remodeling and activation of MAP kinases pathway enzymes.
30470954	4	54	theme	further	842:848	arg1	eosinophils					850:860	further eosinophils	842:860	further eosinophils	842:860	METHODS Inflammatory cell infiltration in lungs was measured by flow cytometry and further eosinophils were especially measured by immunofluorescence detection of major basic protein (MBP) as marker of eosinophilc granule protein.
30470954	3	55	theme	structural	739:748	arg1	changes					750:756	airway structural changes	732:756	airway structural changes	732:756	Curcumin (diferuloylmethane), well known for its anti-inflammatory potential, was administered through intranasal route 1 h before LPS and OVA (ovalbumin) exposure to evaluate its efficacy against airway structural changes.
30470954	0	56	theme	MMP-9	141:145	arg1	expression					148:157	matrixmetalloproteinase-9 (MMP-9) expression	114:157	matrixmetalloproteinase-9 (MMP-9) expression	114:157	Intranasal curcumin protects against LPS-induced airway remodeling by modulating toll-like receptor-4 (TLR-4) and matrixmetalloproteinase-9 (MMP-9) expression via affecting MAP kinases in mouse model.
30470954	1	57	theme	OBJECTIVE	201:209	arg1	infections					221:230	OBJECTIVE Bacterial infections	201:230	OBJECTIVE Bacterial infections	201:230	OBJECTIVE Bacterial infections can exacerbate asthmatic inflammation depending on lipopolysaccharide (LPS) composition, the outermost component of cell wall, its exposure timings as well as host's immune status.
30470954	4	58	theme	basic	928:932	arg1	protein					934:940	major basic protein	922:940	major basic protein (MBP)	922:946	METHODS Inflammatory cell infiltration in lungs was measured by flow cytometry and further eosinophils were especially measured by immunofluorescence detection of major basic protein (MBP) as marker of eosinophilc granule protein.
30470954	4	58	theme	basic	928:932	arg1	MBP					943:945	MBP	943:945	MBP	943:945	METHODS Inflammatory cell infiltration in lungs was measured by flow cytometry and further eosinophils were especially measured by immunofluorescence detection of major basic protein (MBP) as marker of eosinophilc granule protein.
30470954	10	59	theme	airway	1785:1790	arg1	proteins					1803:1810	airway remodeling proteins	1785:1810	airway remodeling proteins	1785:1810	Intranasal curcumin pretreatment had significantly suppressed inflammatory mediators and airway remodeling proteins.
30470954	2	60	theme	asthmatic	518:526	arg1	model					528:532	an asthmatic model	515:532	an asthmatic model	515:532	In present study, Balb/c mice were exposed to antigen (ovalbumin) and LPS simultaneously to establish an asthmatic model.
30470954	8	61	used	used	1399:1402	arg2	level					1356:1360	Hydroxyproline level	1341:1360	Hydroxyproline level	1341:1360	Hydroxyproline level and masson's trichrome staining were used to evaluate collagen deposition in lung.
30470954	8	61	used	used	1399:1402	arg2	staining					1385:1392	masson's trichrome staining	1366:1392	masson's trichrome staining	1366:1392	Hydroxyproline level and masson's trichrome staining were used to evaluate collagen deposition in lung.
30470954	4	62	theme	immunofluorescence	890:907	arg1	detection					909:917	immunofluorescence detection	890:917	immunofluorescence detection of major basic protein (MBP)	890:946	METHODS Inflammatory cell infiltration in lungs was measured by flow cytometry and further eosinophils were especially measured by immunofluorescence detection of major basic protein (MBP) as marker of eosinophilc granule protein.
30470954	7	63	theme	western	1323:1329	arg1	blotting					1331:1338	western blotting	1323:1338	western blotting	1323:1338	Protein expression of MAP kinases (P-ERK, P-JNK, P-p38), TLR-4, Cox-2, Lox-5 and Eotaxin was measured by western blotting.
30470954	9	64	theme	pathway	1679:1685	arg1	enzymes					1687:1693	pathway enzymes	1679:1693	pathway enzymes	1679:1693	RESULTS Exposure to LPS (0.1 µg) exacerbates airway inflammation and induces structural changes in lungs by enhanced ROS production, collagen deposition, expression of genes involved in airway remodeling and activation of MAP kinases pathway enzymes.
30470954	0	65	theme	toll-like	81:89	arg1	receptor-4					91:100	toll-like receptor-4	81:100	toll-like receptor-4 (TLR-4)	81:108	Intranasal curcumin protects against LPS-induced airway remodeling by modulating toll-like receptor-4 (TLR-4) and matrixmetalloproteinase-9 (MMP-9) expression via affecting MAP kinases in mouse model.
30470954	0	65	theme	toll-like	81:89	arg1	TLR-4					103:107	TLR-4	103:107	TLR-4	103:107	Intranasal curcumin protects against LPS-induced airway remodeling by modulating toll-like receptor-4 (TLR-4) and matrixmetalloproteinase-9 (MMP-9) expression via affecting MAP kinases in mouse model.
30470954	11	66	theme	airway	1986:1991	arg1	remodeling					1993:2002	airway remodeling	1986:2002	airway remodeling	1986:2002	CONCLUSION Our results strongly suggest that intranasal curcumin effectively protects LPS-induced airway inflammation and structural changes by modulating genes involved in airway remodeling in safer way; hence, it can be considered as supplementary alternative towards asthma treatments.
30470954	4	67	theme	eosinophilc	961:971	arg1	protein					981:987	eosinophilc granule protein	961:987	eosinophilc granule protein	961:987	METHODS Inflammatory cell infiltration in lungs was measured by flow cytometry and further eosinophils were especially measured by immunofluorescence detection of major basic protein (MBP) as marker of eosinophilc granule protein.
30470954	4	68	theme	protein	981:987	arg1	marker					951:956	marker	951:956	marker of eosinophilc granule protein	951:987	METHODS Inflammatory cell infiltration in lungs was measured by flow cytometry and further eosinophils were especially measured by immunofluorescence detection of major basic protein (MBP) as marker of eosinophilc granule protein.
30470954	4	68	theme	protein	981:987	arg1	METHODS					759:765	METHODS	759:765	METHODS Inflammatory cell infiltration in lungs was measured by flow cytometry and further eosinophils	759:860	METHODS Inflammatory cell infiltration in lungs was measured by flow cytometry and further eosinophils were especially measured by immunofluorescence detection of major basic protein (MBP) as marker of eosinophilc granule protein.
30470954	4	69	theme	protein	934:940	arg1	detection					909:917	immunofluorescence detection	890:917	immunofluorescence detection of major basic protein (MBP)	890:946	METHODS Inflammatory cell infiltration in lungs was measured by flow cytometry and further eosinophils were especially measured by immunofluorescence detection of major basic protein (MBP) as marker of eosinophilc granule protein.
30470954	8	70	from	deposition	1425:1434	arg1	lung					1439:1442	lung	1439:1442	lung	1439:1442	Hydroxyproline level and masson's trichrome staining were used to evaluate collagen deposition in lung.
30470954	1	71	theme	exposure	363:370	arg1	composition					308:318	lipopolysaccharide (LPS) composition	283:318	lipopolysaccharide (LPS) composition	283:318	OBJECTIVE Bacterial infections can exacerbate asthmatic inflammation depending on lipopolysaccharide (LPS) composition, the outermost component of cell wall, its exposure timings as well as host's immune status.
30470954	1	71	theme	exposure	363:370	arg1	timings					372:378	its exposure timings	359:378	its exposure timings as well as host's immune status	359:410	OBJECTIVE Bacterial infections can exacerbate asthmatic inflammation depending on lipopolysaccharide (LPS) composition, the outermost component of cell wall, its exposure timings as well as host's immune status.
30470954	0	72	theme	matrixmetalloproteinase-9	114:138	arg1	expression					148:157	matrixmetalloproteinase-9 (MMP-9) expression	114:157	matrixmetalloproteinase-9 (MMP-9) expression	114:157	Intranasal curcumin protects against LPS-induced airway remodeling by modulating toll-like receptor-4 (TLR-4) and matrixmetalloproteinase-9 (MMP-9) expression via affecting MAP kinases in mouse model.
30470954	11	73	dep	CONCLUSION	1813:1822	arg1	suggest					1845:1851	suggest	1845:1851	suggest that intranasal curcumin effectively protects LPS-induced airway inflammation and structural changes by modulating genes involved in airway remodeling in safer way; hence, it can be considered as supplementary alternative towards asthma treatments	1845:2099	CONCLUSION Our results strongly suggest that intranasal curcumin effectively protects LPS-induced airway inflammation and structural changes by modulating genes involved in airway remodeling in safer way; hence, it can be considered as supplementary alternative towards asthma treatments.
30470954	11	74	theme	LPS-induced	1899:1909	arg1	inflammation					1918:1929	LPS-induced airway inflammation	1899:1929	LPS-induced airway inflammation	1899:1929	CONCLUSION Our results strongly suggest that intranasal curcumin effectively protects LPS-induced airway inflammation and structural changes by modulating genes involved in airway remodeling in safer way; hence, it can be considered as supplementary alternative towards asthma treatments.
30470954	3	75	theme	intranasal	638:647	arg1	1 h					655:657	intranasal route 1 h	638:657	intranasal route 1 h before LPS and OVA (ovalbumin) exposure	638:697	Curcumin (diferuloylmethane), well known for its anti-inflammatory potential, was administered through intranasal route 1 h before LPS and OVA (ovalbumin) exposure to evaluate its efficacy against airway structural changes.
30470954	2	76	theme	present	416:422	arg1	study					424:428	present study	416:428	present study	416:428	In present study, Balb/c mice were exposed to antigen (ovalbumin) and LPS simultaneously to establish an asthmatic model.
30470954	0	77	theme	airway	49:54	arg1	remodeling					56:65	LPS-induced airway remodeling	37:65	LPS-induced airway remodeling	37:65	Intranasal curcumin protects against LPS-induced airway remodeling by modulating toll-like receptor-4 (TLR-4) and matrixmetalloproteinase-9 (MMP-9) expression via affecting MAP kinases in mouse model.
30470954	5	78	theme	oxygen	1016:1021	arg1	species					1023:1029	reactive oxygen species	1007:1029	reactive oxygen species (ROS)	1007:1035	We also measured reactive oxygen species (ROS) in BALF by spectrofluorometry.
30470954	5	78	theme	oxygen	1016:1021	arg1	ROS					1032:1034	ROS	1032:1034	ROS	1032:1034	We also measured reactive oxygen species (ROS) in BALF by spectrofluorometry.
30470954	11	79	theme	structural	1935:1944	arg1	changes					1946:1952	structural changes	1935:1952	structural changes	1935:1952	CONCLUSION Our results strongly suggest that intranasal curcumin effectively protects LPS-induced airway inflammation and structural changes by modulating genes involved in airway remodeling in safer way; hence, it can be considered as supplementary alternative towards asthma treatments.
30470954	11	80	theme	supplementary	2049:2061	arg1	alternative					2063:2073	supplementary alternative	2049:2073	supplementary alternative towards asthma treatments	2049:2099	CONCLUSION Our results strongly suggest that intranasal curcumin effectively protects LPS-induced airway inflammation and structural changes by modulating genes involved in airway remodeling in safer way; hence, it can be considered as supplementary alternative towards asthma treatments.
30470954	11	80	theme	supplementary	2049:2061	arg1	it					2025:2026	it	2025:2026	it	2025:2026	CONCLUSION Our results strongly suggest that intranasal curcumin effectively protects LPS-induced airway inflammation and structural changes by modulating genes involved in airway remodeling in safer way; hence, it can be considered as supplementary alternative towards asthma treatments.
30470954	1	81	theme	cell	348:351	arg1	wall					353:356	cell wall	348:356	cell wall	348:356	OBJECTIVE Bacterial infections can exacerbate asthmatic inflammation depending on lipopolysaccharide (LPS) composition, the outermost component of cell wall, its exposure timings as well as host's immune status.
30470954	9	82	theme	enhanced	1553:1560	arg1	production					1566:1575	enhanced ROS production	1553:1575	enhanced ROS production	1553:1575	RESULTS Exposure to LPS (0.1 µg) exacerbates airway inflammation and induces structural changes in lungs by enhanced ROS production, collagen deposition, expression of genes involved in airway remodeling and activation of MAP kinases pathway enzymes.
30470954	7	83	theme	MAP	1240:1242	arg1	Lox-5					1289:1293	Lox-5	1289:1293	Lox-5	1289:1293	Protein expression of MAP kinases (P-ERK, P-JNK, P-p38), TLR-4, Cox-2, Lox-5 and Eotaxin was measured by western blotting.
30470954	7	83	theme	MAP	1240:1242	arg1	kinases					1244:1250	MAP kinases	1240:1250	MAP kinases (P-ERK, P-JNK, P-p38)	1240:1272	Protein expression of MAP kinases (P-ERK, P-JNK, P-p38), TLR-4, Cox-2, Lox-5 and Eotaxin was measured by western blotting.
30470954	7	83	theme	MAP	1240:1242	arg1	Cox-2					1282:1286	Cox-2	1282:1286	Cox-2	1282:1286	Protein expression of MAP kinases (P-ERK, P-JNK, P-p38), TLR-4, Cox-2, Lox-5 and Eotaxin was measured by western blotting.
30470954	7	83	theme	MAP	1240:1242	arg1	TLR-4					1275:1279	TLR-4	1275:1279	TLR-4	1275:1279	Protein expression of MAP kinases (P-ERK, P-JNK, P-p38), TLR-4, Cox-2, Lox-5 and Eotaxin was measured by western blotting.
30470954	7	83	theme	MAP	1240:1242	arg1	Eotaxin					1299:1305	Eotaxin	1299:1305	Eotaxin	1299:1305	Protein expression of MAP kinases (P-ERK, P-JNK, P-p38), TLR-4, Cox-2, Lox-5 and Eotaxin was measured by western blotting.
30470954	4	84	theme	cell	780:783	arg1	infiltration					785:796	Inflammatory cell infiltration	767:796	Inflammatory cell infiltration in lungs	767:805	METHODS Inflammatory cell infiltration in lungs was measured by flow cytometry and further eosinophils were especially measured by immunofluorescence detection of major basic protein (MBP) as marker of eosinophilc granule protein.
30470954	1	85	theme	asthmatic	247:255	arg1	inflammation					257:268	asthmatic inflammation	247:268	asthmatic inflammation	247:268	OBJECTIVE Bacterial infections can exacerbate asthmatic inflammation depending on lipopolysaccharide (LPS) composition, the outermost component of cell wall, its exposure timings as well as host's immune status.
30470954	6	86	theme	gelatin	1100:1106	arg1	zymography					1108:1117	gelatin zymography	1100:1117	gelatin zymography	1100:1117	MMP-9 activity was evaluated by gelatin zymography and mRNA expressions of MMP-9, TIMP-1, TGF-β1, IL-13, Collagen-1 and TLR-4 were measured in lungs.
30470954	3	87	theme	ovalbumin	679:687	arg1	exposure					690:697	OVA (ovalbumin) exposure	674:697	OVA (ovalbumin) exposure	674:697	Curcumin (diferuloylmethane), well known for its anti-inflammatory potential, was administered through intranasal route 1 h before LPS and OVA (ovalbumin) exposure to evaluate its efficacy against airway structural changes.
31050258	7	0	theme	cellulose-degrading	1211:1229	arg1	enzymes					1231:1237	the cellulose-degrading enzymes	1207:1237	the cellulose-degrading enzymes	1207:1237	Optimization of biosynthesis of the cellulose-degrading enzymes indicated that optimal temperature of this process fluctuated in the range of 21-42°C (depending on the strain and carbon source).
31050258	9	1	theme	commercial	1871:1880	arg1	applications					1882:1893	commercial applications	1871:1893	commercial applications	1871:1893	These experiments show that microorganisms isolated from natural environmental samples have unique properties and potential for commercial applications (e.g. for biopreparations production).
31050258	8	2	theme	desirability	1576:1587	arg1	methodology					1598:1608	a desirability function methodology	1574:1608	a desirability function methodology	1574:1608	The highest cellulolytic activity was observed for the isolates designed as 4/7 (identified as Bacillus subtilis) and 4/18 (identified as Bacillus licheniformis) in a temperature of 32°C. With the use of a desirability function methodology, the optimal medium composition to achieve a simple, cost-efficient process of cellulases production was developed for both strains.
31050258	1	3	theme	glucose	241:247	arg1	isomerase					249:257	glucose isomerase	241:257	glucose isomerase	241:257	Bacteria from the genus Bacillus are a rich source of commercial enzymes, including amylases, proteases, cellulases, glucose isomerase, and pullulanase.
31050258	4	4	theme	postharvest	837:847	arg1	leftovers					849:857	postharvest leftovers	837:857	postharvest leftovers	837:857	In this study, we were looking for the bacteria isolated from the natural environment with the high cellulolytic potential, which could be used as components of a biopreparation to accelerate decomposition of postharvest leftovers in agriculture.
31050258	14	5	theme	diffusion	2837:2845	arg1	method					2847:2852	the diffusion method	2833:2852	the diffusion method	2833:2852	The 214 bacterial strains were isolated from environmental samples rich in cellulose and their ability to synthesize cellulases were examined using the diffusion method.
31050258	17	6	theme	optimal	3425:3431	arg1	composition					3440:3450	the optimal medium composition	3421:3450	the optimal medium composition to achieve a simple, cost-efficient process of cellulases production	3421:3519	The highest cellulolytic activity was observed for the isolates designed as 4/7 (identified as Bacillus subtilis) and 4/18 (identified as Bacillus licheniformis) in a temperature of 32°C. With the use of a desirability function methodology, the optimal medium composition to achieve a simple, cost-efficient process of cellulases production was developed for both strains.
31050258	8	7	theme	methodology	1598:1608	arg1	use					1567:1569	the use	1563:1569	the use of a desirability function methodology	1563:1608	The highest cellulolytic activity was observed for the isolates designed as 4/7 (identified as Bacillus subtilis) and 4/18 (identified as Bacillus licheniformis) in a temperature of 32°C. With the use of a desirability function methodology, the optimal medium composition to achieve a simple, cost-efficient process of cellulases production was developed for both strains.
31050258	18	8	theme	biopreparations	3715:3729	arg1	production					3731:3740	biopreparations production	3715:3740	biopreparations production	3715:3740	These experiments show that microorganisms isolated from natural environmental samples have unique properties and potential for commercial applications (e.g. for biopreparations production).
31050258	13	9	theme	natural	2504:2510	arg1	environment					2512:2522	the natural environment	2500:2522	the natural environment	2500:2522	In this study, we were looking for the bacteria isolated from the natural environment with the high cellulolytic potential, which could be used as components of a biopreparation to accelerate decomposition of postharvest leftovers in agriculture.
31050258	13	10	theme	postharvest	2647:2657	arg1	leftovers					2659:2667	postharvest leftovers	2647:2667	postharvest leftovers	2647:2667	In this study, we were looking for the bacteria isolated from the natural environment with the high cellulolytic potential, which could be used as components of a biopreparation to accelerate decomposition of postharvest leftovers in agriculture.
31050258	17	11	located	observed	3218:3225	arg1	temperature					3347:3357	a temperature	3345:3357	a temperature of 32°C. With the use of a desirability function methodology	3345:3418	The highest cellulolytic activity was observed for the isolates designed as 4/7 (identified as Bacillus subtilis) and 4/18 (identified as Bacillus licheniformis) in a temperature of 32°C. With the use of a desirability function methodology, the optimal medium composition to achieve a simple, cost-efficient process of cellulases production was developed for both strains.
31050258	17	11	located	observed	3218:3225	arg2	activity					3205:3212	The highest cellulolytic activity	3180:3212	The highest cellulolytic activity	3180:3212	The highest cellulolytic activity was observed for the isolates designed as 4/7 (identified as Bacillus subtilis) and 4/18 (identified as Bacillus licheniformis) in a temperature of 32°C. With the use of a desirability function methodology, the optimal medium composition to achieve a simple, cost-efficient process of cellulases production was developed for both strains.
31050258	9	12	dep	have	1830:1833	arg1	e.g.					1896:1899	e.g.	1896:1899	e.g.	1896:1899	These experiments show that microorganisms isolated from natural environmental samples have unique properties and potential for commercial applications (e.g. for biopreparations production).
31050258	17	13	theme	32°C.	3362:3366	arg1	temperature					3347:3357	a temperature	3345:3357	a temperature of 32°C. With the use of a desirability function methodology	3345:3418	The highest cellulolytic activity was observed for the isolates designed as 4/7 (identified as Bacillus subtilis) and 4/18 (identified as Bacillus licheniformis) in a temperature of 32°C. With the use of a desirability function methodology, the optimal medium composition to achieve a simple, cost-efficient process of cellulases production was developed for both strains.
31050258	17	14	theme	cellulolytic	3192:3203	arg1	activity					3205:3212	The highest cellulolytic activity	3180:3212	The highest cellulolytic activity	3180:3212	The highest cellulolytic activity was observed for the isolates designed as 4/7 (identified as Bacillus subtilis) and 4/18 (identified as Bacillus licheniformis) in a temperature of 32°C. With the use of a desirability function methodology, the optimal medium composition to achieve a simple, cost-efficient process of cellulases production was developed for both strains.
31050258	8	15	theme	cellulases	1689:1698	arg1	production					1700:1709	cellulases production	1689:1709	cellulases production	1689:1709	The highest cellulolytic activity was observed for the isolates designed as 4/7 (identified as Bacillus subtilis) and 4/18 (identified as Bacillus licheniformis) in a temperature of 32°C. With the use of a desirability function methodology, the optimal medium composition to achieve a simple, cost-efficient process of cellulases production was developed for both strains.
31050258	1	16	theme	commercial	178:187	arg1	proteases					218:226	proteases	218:226	proteases	218:226	Bacteria from the genus Bacillus are a rich source of commercial enzymes, including amylases, proteases, cellulases, glucose isomerase, and pullulanase.
31050258	1	16	theme	commercial	178:187	arg1	cellulases					229:238	cellulases	229:238	cellulases	229:238	Bacteria from the genus Bacillus are a rich source of commercial enzymes, including amylases, proteases, cellulases, glucose isomerase, and pullulanase.
31050258	1	16	theme	commercial	178:187	arg1	pullulanase					264:274	pullulanase	264:274	pullulanase	264:274	Bacteria from the genus Bacillus are a rich source of commercial enzymes, including amylases, proteases, cellulases, glucose isomerase, and pullulanase.
31050258	1	16	theme	commercial	178:187	arg1	enzymes					189:195	commercial enzymes	178:195	commercial enzymes	178:195	Bacteria from the genus Bacillus are a rich source of commercial enzymes, including amylases, proteases, cellulases, glucose isomerase, and pullulanase.
31050258	1	16	theme	commercial	178:187	arg1	amylases					208:215	amylases	208:215	amylases	208:215	Bacteria from the genus Bacillus are a rich source of commercial enzymes, including amylases, proteases, cellulases, glucose isomerase, and pullulanase.
31050258	1	16	theme	commercial	178:187	arg1	isomerase					249:257	glucose isomerase	241:257	glucose isomerase	241:257	Bacteria from the genus Bacillus are a rich source of commercial enzymes, including amylases, proteases, cellulases, glucose isomerase, and pullulanase.
31050258	12	17	theme	world	2431:2435	arg1	regions					2416:2422	various regions	2408:2422	various regions of the world	2408:2435	Many of cellulose-degrading microorganisms have been isolated so far and characterized in various regions of the world.
31050258	3	18	theme	cellulose-degrading	516:534	arg1	microorganisms					536:549	cellulose-degrading microorganisms	516:549	cellulose-degrading microorganisms	516:549	Many of cellulose-degrading microorganisms have been isolated so far and characterized in various regions of the world.
31050258	8	19	located	observed	1408:1415	arg1	temperature					1537:1547	a temperature	1535:1547	a temperature of 32°C. With the use of a desirability function methodology	1535:1608	The highest cellulolytic activity was observed for the isolates designed as 4/7 (identified as Bacillus subtilis) and 4/18 (identified as Bacillus licheniformis) in a temperature of 32°C. With the use of a desirability function methodology, the optimal medium composition to achieve a simple, cost-efficient process of cellulases production was developed for both strains.
31050258	8	19	located	observed	1408:1415	arg2	activity					1395:1402	The highest cellulolytic activity	1370:1402	The highest cellulolytic activity	1370:1402	The highest cellulolytic activity was observed for the isolates designed as 4/7 (identified as Bacillus subtilis) and 4/18 (identified as Bacillus licheniformis) in a temperature of 32°C. With the use of a desirability function methodology, the optimal medium composition to achieve a simple, cost-efficient process of cellulases production was developed for both strains.
31050258	15	20	theme	zone	2912:2915	arg1	diameter					2891:2898	the highest diameter	2879:2898	the highest diameter of clearing zone both for biomass and supernatant	2879:2948	Six strains, which have the highest diameter of clearing zone both for biomass and supernatant, were selected for identification.
31050258	18	21	theme	environmental	3618:3630	arg1	samples					3632:3638	natural environmental samples	3610:3638	natural environmental samples	3610:3638	These experiments show that microorganisms isolated from natural environmental samples have unique properties and potential for commercial applications (e.g. for biopreparations production).
31050258	10	22	theme	rich	1973:1976	arg1	Bacteria					1934:1941	Bacteria	1934:1941	Bacteria from the genus Bacillus	1934:1965	Bacteria from the genus Bacillus are a rich source of commercial enzymes, including amylases, proteases, cellulases, glucose isomerase, and pullulanase.
31050258	10	22	theme	rich	1973:1976	arg1	source					1978:1983	a rich source	1971:1983	a rich source of commercial enzymes, including amylases, proteases, cellulases, glucose isomerase, and pullulanase	1971:2084	Bacteria from the genus Bacillus are a rich source of commercial enzymes, including amylases, proteases, cellulases, glucose isomerase, and pullulanase.
31050258	9	23	attach	isolated	1786:1793	arg1	samples					1822:1828	natural environmental samples	1800:1828	natural environmental samples	1800:1828	These experiments show that microorganisms isolated from natural environmental samples have unique properties and potential for commercial applications (e.g. for biopreparations production).
31050258	9	23	attach	isolated	1786:1793	arg2	microorganisms					1771:1784	microorganisms	1771:1784	microorganisms isolated from natural environmental samples	1771:1828	These experiments show that microorganisms isolated from natural environmental samples have unique properties and potential for commercial applications (e.g. for biopreparations production).
31050258	6	24	contain	have	1064:1067	arg1	strains					1049:1055	Six strains	1045:1055	Six strains	1045:1055	Six strains, which have the highest diameter of clearing zone both for biomass and supernatant, were selected for identification.
31050258	6	24	contain	have	1064:1067	arg2	diameter					1081:1088	the highest diameter	1069:1088	the highest diameter of clearing zone both for biomass and supernatant	1069:1138	Six strains, which have the highest diameter of clearing zone both for biomass and supernatant, were selected for identification.
31050258	8	25	dep	composition	1630:1640	arg1	achieve					1645:1651	achieve	1645:1651	to achieve a simple, cost-efficient process of cellulases production	1642:1709	The highest cellulolytic activity was observed for the isolates designed as 4/7 (identified as Bacillus subtilis) and 4/18 (identified as Bacillus licheniformis) in a temperature of 32°C. With the use of a desirability function methodology, the optimal medium composition to achieve a simple, cost-efficient process of cellulases production was developed for both strains.
31050258	17	26	dep	4/7	3256:3258	arg1	identified					3261:3270	identified	3261:3270	identified as Bacillus subtilis	3261:3291	The highest cellulolytic activity was observed for the isolates designed as 4/7 (identified as Bacillus subtilis) and 4/18 (identified as Bacillus licheniformis) in a temperature of 32°C. With the use of a desirability function methodology, the optimal medium composition to achieve a simple, cost-efficient process of cellulases production was developed for both strains.
31050258	5	27	theme	diffusion	1027:1035	arg1	method					1037:1042	the diffusion method	1023:1042	the diffusion method	1023:1042	The 214 bacterial strains were isolated from environmental samples rich in cellulose and their ability to synthesize cellulases were examined using the diffusion method.
31050258	3	28	theme	various	598:604	arg1	regions					606:612	various regions	598:612	various regions of the world	598:625	Many of cellulose-degrading microorganisms have been isolated so far and characterized in various regions of the world.
31050258	15	29	theme	highest	2883:2889	arg1	diameter					2891:2898	the highest diameter	2879:2898	the highest diameter of clearing zone both for biomass and supernatant	2879:2948	Six strains, which have the highest diameter of clearing zone both for biomass and supernatant, were selected for identification.
31050258	10	30	theme	enzymes	1999:2005	arg1	Bacteria					1934:1941	Bacteria	1934:1941	Bacteria from the genus Bacillus	1934:1965	Bacteria from the genus Bacillus are a rich source of commercial enzymes, including amylases, proteases, cellulases, glucose isomerase, and pullulanase.
31050258	10	30	theme	enzymes	1999:2005	arg1	source					1978:1983	a rich source	1971:1983	a rich source of commercial enzymes, including amylases, proteases, cellulases, glucose isomerase, and pullulanase	1971:2084	Bacteria from the genus Bacillus are a rich source of commercial enzymes, including amylases, proteases, cellulases, glucose isomerase, and pullulanase.
31050258	0	31	theme	Cellulolytic	52:63	arg1	Potential					65:73	Cellulolytic Potential	52:73	Cellulolytic Potential of Microbiota	52:87	The Influence of Temperature and Nitrogen Source on Cellulolytic Potential of Microbiota Isolated from Natural Environment.
31050258	14	32	attach	isolated	2716:2723	arg2	strains					2703:2709	The 214 bacterial strains	2685:2709	The 214 bacterial strains	2685:2709	The 214 bacterial strains were isolated from environmental samples rich in cellulose and their ability to synthesize cellulases were examined using the diffusion method.
31050258	14	32	attach	isolated	2716:2723	arg1	samples					2744:2750	environmental samples	2730:2750	environmental samples rich in cellulose	2730:2768	The 214 bacterial strains were isolated from environmental samples rich in cellulose and their ability to synthesize cellulases were examined using the diffusion method.
31050258	7	33	theme	biosynthesis	1191:1202	arg1	Optimization					1175:1186	Optimization	1175:1186	Optimization of biosynthesis of the cellulose-degrading enzymes	1175:1237	Optimization of biosynthesis of the cellulose-degrading enzymes indicated that optimal temperature of this process fluctuated in the range of 21-42°C (depending on the strain and carbon source).
31050258	18	34	contain	have	3640:3643	arg2	potential					3667:3675	potential	3667:3675	potential for commercial applications	3667:3703	These experiments show that microorganisms isolated from natural environmental samples have unique properties and potential for commercial applications (e.g. for biopreparations production).
31050258	18	34	contain	have	3640:3643	arg2	properties					3652:3661	unique properties	3645:3661	unique properties	3645:3661	These experiments show that microorganisms isolated from natural environmental samples have unique properties and potential for commercial applications (e.g. for biopreparations production).
31050258	18	34	contain	have	3640:3643	arg1	microorganisms					3581:3594	microorganisms	3581:3594	microorganisms isolated from natural environmental samples	3581:3638	These experiments show that microorganisms isolated from natural environmental samples have unique properties and potential for commercial applications (e.g. for biopreparations production).
31050258	14	35	theme	rich	2752:2755	arg1	samples					2744:2750	environmental samples	2730:2750	environmental samples rich in cellulose	2730:2768	The 214 bacterial strains were isolated from environmental samples rich in cellulose and their ability to synthesize cellulases were examined using the diffusion method.
31050258	17	36	theme	cellulases	3499:3508	arg1	production					3510:3519	cellulases production	3499:3519	cellulases production	3499:3519	The highest cellulolytic activity was observed for the isolates designed as 4/7 (identified as Bacillus subtilis) and 4/18 (identified as Bacillus licheniformis) in a temperature of 32°C. With the use of a desirability function methodology, the optimal medium composition to achieve a simple, cost-efficient process of cellulases production was developed for both strains.
31050258	1	37	from	genus	142:146	arg1	Bacteria					124:131	Bacteria	124:131	Bacteria from the genus Bacillus	124:155	Bacteria from the genus Bacillus are a rich source of commercial enzymes, including amylases, proteases, cellulases, glucose isomerase, and pullulanase.
31050258	1	37	from	genus	142:146	arg1	source					168:173	a rich source	161:173	a rich source of commercial enzymes, including amylases, proteases, cellulases, glucose isomerase, and pullulanase	161:274	Bacteria from the genus Bacillus are a rich source of commercial enzymes, including amylases, proteases, cellulases, glucose isomerase, and pullulanase.
31050258	5	38	theme	rich	942:945	arg1	samples					934:940	environmental samples	920:940	environmental samples rich in cellulose	920:958	The 214 bacterial strains were isolated from environmental samples rich in cellulose and their ability to synthesize cellulases were examined using the diffusion method.
31050258	2	39	theme	synthesis	479:487	arg1	level					463:467	the level	459:467	the level of enzyme synthesis	459:487	Cellulases account for 15% of the global market of industrial enzymes; thus, new microorganisms producing cellulases in a higher concentration and new ingredients, which can enhance the level of enzyme synthesis, are still needed.
31050258	16	40	theme	optimal	3064:3070	arg1	temperature					3072:3082	optimal temperature	3064:3082	optimal temperature of this process	3064:3098	Optimization of biosynthesis of the cellulose-degrading enzymes indicated that optimal temperature of this process fluctuated in the range of 21–42°C (depending on the strain and carbon source).
31050258	17	41	dep	4/18	3298:3301	arg1	identified					3304:3313	identified	3304:3313	identified as Bacillus licheniformis	3304:3339	The highest cellulolytic activity was observed for the isolates designed as 4/7 (identified as Bacillus subtilis) and 4/18 (identified as Bacillus licheniformis) in a temperature of 32°C. With the use of a desirability function methodology, the optimal medium composition to achieve a simple, cost-efficient process of cellulases production was developed for both strains.
31050258	4	42	theme	cellulolytic	728:739	arg1	potential					741:749	the high cellulolytic potential	719:749	the high cellulolytic potential	719:749	In this study, we were looking for the bacteria isolated from the natural environment with the high cellulolytic potential, which could be used as components of a biopreparation to accelerate decomposition of postharvest leftovers in agriculture.
31050258	4	42	theme	cellulolytic	728:739	arg1	components					775:784	components	775:784	components of a biopreparation	775:804	In this study, we were looking for the bacteria isolated from the natural environment with the high cellulolytic potential, which could be used as components of a biopreparation to accelerate decomposition of postharvest leftovers in agriculture.
31050258	16	43	dep	fluctuated	3100:3109	arg1	source					3171:3176	the strain and carbon source	3149:3176	source	3171:3176	Optimization of biosynthesis of the cellulose-degrading enzymes indicated that optimal temperature of this process fluctuated in the range of 21–42°C (depending on the strain and carbon source).
31050258	0	44	theme	Natural	103:109	arg1	Environment					111:121	Natural Environment	103:121	Natural Environment	103:121	The Influence of Temperature and Nitrogen Source on Cellulolytic Potential of Microbiota Isolated from Natural Environment.
31050258	13	45	attach	isolated	2486:2493	arg1	environment					2512:2522	the natural environment	2500:2522	the natural environment	2500:2522	In this study, we were looking for the bacteria isolated from the natural environment with the high cellulolytic potential, which could be used as components of a biopreparation to accelerate decomposition of postharvest leftovers in agriculture.
31050258	13	45	attach	isolated	2486:2493	arg2	bacteria					2477:2484	the bacteria	2473:2484	the bacteria isolated from the natural environment with the high cellulolytic potential, which could be used as components of a biopreparation to accelerate decomposition of postharvest leftovers in agriculture	2473:2682	In this study, we were looking for the bacteria isolated from the natural environment with the high cellulolytic potential, which could be used as components of a biopreparation to accelerate decomposition of postharvest leftovers in agriculture.
31050258	2	46	theme	new	424:426	arg1	ingredients					428:438	new ingredients	424:438	new ingredients	424:438	Cellulases account for 15% of the global market of industrial enzymes; thus, new microorganisms producing cellulases in a higher concentration and new ingredients, which can enhance the level of enzyme synthesis, are still needed.
31050258	18	47	attach	isolated	3596:3603	arg2	microorganisms					3581:3594	microorganisms	3581:3594	microorganisms isolated from natural environmental samples	3581:3638	These experiments show that microorganisms isolated from natural environmental samples have unique properties and potential for commercial applications (e.g. for biopreparations production).
31050258	18	47	attach	isolated	3596:3603	arg1	samples					3632:3638	natural environmental samples	3610:3638	natural environmental samples	3610:3638	These experiments show that microorganisms isolated from natural environmental samples have unique properties and potential for commercial applications (e.g. for biopreparations production).
31050258	13	48	used	used	2577:2580	arg2	potential					2551:2559	the high cellulolytic potential	2529:2559	the high cellulolytic potential	2529:2559	In this study, we were looking for the bacteria isolated from the natural environment with the high cellulolytic potential, which could be used as components of a biopreparation to accelerate decomposition of postharvest leftovers in agriculture.
31050258	13	48	used	used	2577:2580	arg2	components					2585:2594	components	2585:2594	components of a biopreparation	2585:2614	In this study, we were looking for the bacteria isolated from the natural environment with the high cellulolytic potential, which could be used as components of a biopreparation to accelerate decomposition of postharvest leftovers in agriculture.
31050258	11	49	theme	market	2128:2133	arg1	market					2128:2133	the global market	2117:2133	the global market of industrial enzymes	2117:2155	Cellulases account for 15% of the global market of industrial enzymes; thus, new microorganisms producing cellulases in a higher concentration and new ingredients, which can enhance the level of enzyme synthesis, are still needed.
31050258	11	49	theme	market	2128:2133	arg1	%					2112:2112	15%	2110:2112	15% of the global market of industrial enzymes	2110:2155	Cellulases account for 15% of the global market of industrial enzymes; thus, new microorganisms producing cellulases in a higher concentration and new ingredients, which can enhance the level of enzyme synthesis, are still needed.
31050258	12	50	theme	various	2408:2414	arg1	regions					2416:2422	various regions	2408:2422	various regions of the world	2408:2435	Many of cellulose-degrading microorganisms have been isolated so far and characterized in various regions of the world.
31050258	17	51	theme	medium	3433:3438	arg1	composition					3440:3450	the optimal medium composition	3421:3450	the optimal medium composition to achieve a simple, cost-efficient process of cellulases production	3421:3519	The highest cellulolytic activity was observed for the isolates designed as 4/7 (identified as Bacillus subtilis) and 4/18 (identified as Bacillus licheniformis) in a temperature of 32°C. With the use of a desirability function methodology, the optimal medium composition to achieve a simple, cost-efficient process of cellulases production was developed for both strains.
31050258	8	52	dep	simple	1655:1660	arg1	cost-efficient					1663:1676	cost-efficient	1663:1676	cost-efficient	1663:1676	The highest cellulolytic activity was observed for the isolates designed as 4/7 (identified as Bacillus subtilis) and 4/18 (identified as Bacillus licheniformis) in a temperature of 32°C. With the use of a desirability function methodology, the optimal medium composition to achieve a simple, cost-efficient process of cellulases production was developed for both strains.
31050258	1	53	dep	genus	142:146	arg1	Bacillus					148:155	the genus Bacillus	138:155	the genus Bacillus	138:155	Bacteria from the genus Bacillus are a rich source of commercial enzymes, including amylases, proteases, cellulases, glucose isomerase, and pullulanase.
31050258	12	54	theme	microorganisms	2346:2359	arg1	Many					2318:2321	Many	2318:2321	Many	2318:2321	Many of cellulose-degrading microorganisms have been isolated so far and characterized in various regions of the world.
31050258	12	54	theme	microorganisms	2346:2359	arg1	microorganisms					2346:2359	cellulose-degrading microorganisms	2326:2359	cellulose-degrading microorganisms	2326:2359	Many of cellulose-degrading microorganisms have been isolated so far and characterized in various regions of the world.
31050258	5	55	theme	environmental	920:932	arg1	samples					934:940	environmental samples	920:940	environmental samples rich in cellulose	920:958	The 214 bacterial strains were isolated from environmental samples rich in cellulose and their ability to synthesize cellulases were examined using the diffusion method.
31050258	13	56	theme	leftovers	2659:2667	arg1	decomposition					2630:2642	decomposition	2630:2642	decomposition of postharvest leftovers	2630:2667	In this study, we were looking for the bacteria isolated from the natural environment with the high cellulolytic potential, which could be used as components of a biopreparation to accelerate decomposition of postharvest leftovers in agriculture.
31050258	3	57	theme	world	621:625	arg1	regions					606:612	various regions	598:612	various regions of the world	598:625	Many of cellulose-degrading microorganisms have been isolated so far and characterized in various regions of the world.
31050258	15	58	dep	zone	2912:2915	arg1	both					2917:2920	both	2917:2920	both	2917:2920	Six strains, which have the highest diameter of clearing zone both for biomass and supernatant, were selected for identification.
31050258	17	59	with	temperature	3347:3357	arg1	use					3377:3379	the use	3373:3379	the use of a desirability function methodology	3373:3418	The highest cellulolytic activity was observed for the isolates designed as 4/7 (identified as Bacillus subtilis) and 4/18 (identified as Bacillus licheniformis) in a temperature of 32°C. With the use of a desirability function methodology, the optimal medium composition to achieve a simple, cost-efficient process of cellulases production was developed for both strains.
31050258	11	60	theme	new	2164:2166	arg1	microorganisms					2168:2181	new microorganisms	2164:2181	new microorganisms producing cellulases in a higher concentration and new ingredients, which can enhance the level of enzyme synthesis,	2164:2298	Cellulases account for 15% of the global market of industrial enzymes; thus, new microorganisms producing cellulases in a higher concentration and new ingredients, which can enhance the level of enzyme synthesis, are still needed.
31050258	8	61	theme	cellulolytic	1382:1393	arg1	activity					1395:1402	The highest cellulolytic activity	1370:1402	The highest cellulolytic activity	1370:1402	The highest cellulolytic activity was observed for the isolates designed as 4/7 (identified as Bacillus subtilis) and 4/18 (identified as Bacillus licheniformis) in a temperature of 32°C. With the use of a desirability function methodology, the optimal medium composition to achieve a simple, cost-efficient process of cellulases production was developed for both strains.
31050258	14	62	from	cellulose	2760:2768	arg1	rich					2752:2755	rich	2752:2755	rich	2752:2755	The 214 bacterial strains were isolated from environmental samples rich in cellulose and their ability to synthesize cellulases were examined using the diffusion method.
31050258	2	63	theme	market	318:323	arg1	market					318:323	the global market	307:323	the global market of industrial enzymes	307:345	Cellulases account for 15% of the global market of industrial enzymes; thus, new microorganisms producing cellulases in a higher concentration and new ingredients, which can enhance the level of enzyme synthesis, are still needed.
31050258	2	63	theme	market	318:323	arg1	%					302:302	15%	300:302	15% of the global market of industrial enzymes	300:345	Cellulases account for 15% of the global market of industrial enzymes; thus, new microorganisms producing cellulases in a higher concentration and new ingredients, which can enhance the level of enzyme synthesis, are still needed.
31050258	0	64	theme	Source	42:47	arg1	Influence					4:12	The Influence	0:12	The Influence of Temperature and Nitrogen Source on Cellulolytic Potential of Microbiota	0:87	The Influence of Temperature and Nitrogen Source on Cellulolytic Potential of Microbiota Isolated from Natural Environment.
31050258	18	65	dep	have	3640:3643	arg1	e.g.					3706:3709	e.g.	3706:3709	e.g.	3706:3709	These experiments show that microorganisms isolated from natural environmental samples have unique properties and potential for commercial applications (e.g. for biopreparations production).
31050258	6	66	theme	clearing	1093:1100	arg1	zone					1102:1105	clearing zone	1093:1105	clearing zone	1093:1105	Six strains, which have the highest diameter of clearing zone both for biomass and supernatant, were selected for identification.
31050258	9	67	theme	unique	1835:1840	arg1	properties					1842:1851	unique properties	1835:1851	unique properties	1835:1851	These experiments show that microorganisms isolated from natural environmental samples have unique properties and potential for commercial applications (e.g. for biopreparations production).
31050258	17	68	theme	function	3399:3406	arg1	methodology					3408:3418	a desirability function methodology	3384:3418	a desirability function methodology	3384:3418	The highest cellulolytic activity was observed for the isolates designed as 4/7 (identified as Bacillus subtilis) and 4/18 (identified as Bacillus licheniformis) in a temperature of 32°C. With the use of a desirability function methodology, the optimal medium composition to achieve a simple, cost-efficient process of cellulases production was developed for both strains.
31050258	7	69	dep	fluctuated	1290:1299	arg1	source					1361:1366	the strain and carbon source	1339:1366	source	1361:1366	Optimization of biosynthesis of the cellulose-degrading enzymes indicated that optimal temperature of this process fluctuated in the range of 21-42°C (depending on the strain and carbon source).
31050258	2	70	from	cellulases	383:392	arg1	concentration					406:418	a higher concentration	397:418	a higher concentration	397:418	Cellulases account for 15% of the global market of industrial enzymes; thus, new microorganisms producing cellulases in a higher concentration and new ingredients, which can enhance the level of enzyme synthesis, are still needed.
31050258	2	70	from	cellulases	383:392	arg1	ingredients					428:438	new ingredients	424:438	new ingredients	424:438	Cellulases account for 15% of the global market of industrial enzymes; thus, new microorganisms producing cellulases in a higher concentration and new ingredients, which can enhance the level of enzyme synthesis, are still needed.
31050258	11	71	theme	new	2234:2236	arg1	ingredients					2238:2248	new ingredients	2234:2248	new ingredients	2234:2248	Cellulases account for 15% of the global market of industrial enzymes; thus, new microorganisms producing cellulases in a higher concentration and new ingredients, which can enhance the level of enzyme synthesis, are still needed.
31050258	6	72	dep	zone	1102:1105	arg1	both					1107:1110	both	1107:1110	both	1107:1110	Six strains, which have the highest diameter of clearing zone both for biomass and supernatant, were selected for identification.
31050258	8	73	theme	optimal	1615:1621	arg1	composition					1630:1640	the optimal medium composition	1611:1640	the optimal medium composition to achieve a simple, cost-efficient process of cellulases production	1611:1709	The highest cellulolytic activity was observed for the isolates designed as 4/7 (identified as Bacillus subtilis) and 4/18 (identified as Bacillus licheniformis) in a temperature of 32°C. With the use of a desirability function methodology, the optimal medium composition to achieve a simple, cost-efficient process of cellulases production was developed for both strains.
31050258	18	74	theme	commercial	3681:3690	arg1	applications					3692:3703	commercial applications	3681:3703	commercial applications	3681:3703	These experiments show that microorganisms isolated from natural environmental samples have unique properties and potential for commercial applications (e.g. for biopreparations production).
31050258	14	75	from	rich	2752:2755	arg1	cellulose					2760:2768	cellulose	2760:2768	cellulose	2760:2768	The 214 bacterial strains were isolated from environmental samples rich in cellulose and their ability to synthesize cellulases were examined using the diffusion method.
31050258	2	76	theme	industrial	328:337	arg1	enzymes					339:345	industrial enzymes	328:345	industrial enzymes	328:345	Cellulases account for 15% of the global market of industrial enzymes; thus, new microorganisms producing cellulases in a higher concentration and new ingredients, which can enhance the level of enzyme synthesis, are still needed.
31050258	5	77	from	cellulose	950:958	arg1	rich					942:945	rich	942:945	rich	942:945	The 214 bacterial strains were isolated from environmental samples rich in cellulose and their ability to synthesize cellulases were examined using the diffusion method.
31050258	7	78	theme	enzymes	1231:1237	arg1	biosynthesis					1191:1202	biosynthesis	1191:1202	biosynthesis of the cellulose-degrading enzymes	1191:1237	Optimization of biosynthesis of the cellulose-degrading enzymes indicated that optimal temperature of this process fluctuated in the range of 21-42°C (depending on the strain and carbon source).
31050258	4	79	attach	isolated	676:683	arg2	bacteria					667:674	the bacteria	663:674	the bacteria isolated from the natural environment with the high cellulolytic potential, which could be used as components of a biopreparation to accelerate decomposition of postharvest leftovers in agriculture	663:872	In this study, we were looking for the bacteria isolated from the natural environment with the high cellulolytic potential, which could be used as components of a biopreparation to accelerate decomposition of postharvest leftovers in agriculture.
31050258	4	79	attach	isolated	676:683	arg1	environment					702:712	the natural environment	690:712	the natural environment	690:712	In this study, we were looking for the bacteria isolated from the natural environment with the high cellulolytic potential, which could be used as components of a biopreparation to accelerate decomposition of postharvest leftovers in agriculture.
31050258	5	80	from	rich	942:945	arg1	cellulose					950:958	cellulose	950:958	cellulose	950:958	The 214 bacterial strains were isolated from environmental samples rich in cellulose and their ability to synthesize cellulases were examined using the diffusion method.
31050258	11	81	theme	industrial	2138:2147	arg1	enzymes					2149:2155	industrial enzymes	2138:2155	industrial enzymes	2138:2155	Cellulases account for 15% of the global market of industrial enzymes; thus, new microorganisms producing cellulases in a higher concentration and new ingredients, which can enhance the level of enzyme synthesis, are still needed.
31050258	8	82	theme	function	1589:1596	arg1	methodology					1598:1608	a desirability function methodology	1574:1608	a desirability function methodology	1574:1608	The highest cellulolytic activity was observed for the isolates designed as 4/7 (identified as Bacillus subtilis) and 4/18 (identified as Bacillus licheniformis) in a temperature of 32°C. With the use of a desirability function methodology, the optimal medium composition to achieve a simple, cost-efficient process of cellulases production was developed for both strains.
31050258	8	83	theme	simple	1655:1660	arg1	process					1678:1684	a simple, cost-efficient process	1653:1684	a simple, cost-efficient process of cellulases production	1653:1709	The highest cellulolytic activity was observed for the isolates designed as 4/7 (identified as Bacillus subtilis) and 4/18 (identified as Bacillus licheniformis) in a temperature of 32°C. With the use of a desirability function methodology, the optimal medium composition to achieve a simple, cost-efficient process of cellulases production was developed for both strains.
31050258	1	84	theme	rich	163:166	arg1	source					168:173	a rich source	161:173	a rich source of commercial enzymes, including amylases, proteases, cellulases, glucose isomerase, and pullulanase	161:274	Bacteria from the genus Bacillus are a rich source of commercial enzymes, including amylases, proteases, cellulases, glucose isomerase, and pullulanase.
31050258	1	84	theme	rich	163:166	arg1	Bacteria					124:131	Bacteria	124:131	Bacteria from the genus Bacillus	124:155	Bacteria from the genus Bacillus are a rich source of commercial enzymes, including amylases, proteases, cellulases, glucose isomerase, and pullulanase.
31050258	13	85	theme	high	2533:2536	arg1	potential					2551:2559	the high cellulolytic potential	2529:2559	the high cellulolytic potential	2529:2559	In this study, we were looking for the bacteria isolated from the natural environment with the high cellulolytic potential, which could be used as components of a biopreparation to accelerate decomposition of postharvest leftovers in agriculture.
31050258	13	85	theme	high	2533:2536	arg1	components					2585:2594	components	2585:2594	components of a biopreparation	2585:2614	In this study, we were looking for the bacteria isolated from the natural environment with the high cellulolytic potential, which could be used as components of a biopreparation to accelerate decomposition of postharvest leftovers in agriculture.
31050258	11	86	theme	global	2121:2126	arg1	market					2128:2133	the global market	2117:2133	the global market of industrial enzymes	2117:2155	Cellulases account for 15% of the global market of industrial enzymes; thus, new microorganisms producing cellulases in a higher concentration and new ingredients, which can enhance the level of enzyme synthesis, are still needed.
31050258	9	87	theme	biopreparations	1905:1919	arg1	production					1921:1930	biopreparations production	1905:1930	biopreparations production	1905:1930	These experiments show that microorganisms isolated from natural environmental samples have unique properties and potential for commercial applications (e.g. for biopreparations production).
31050258	13	88	theme	biopreparation	2601:2614	arg1	potential					2551:2559	the high cellulolytic potential	2529:2559	the high cellulolytic potential	2529:2559	In this study, we were looking for the bacteria isolated from the natural environment with the high cellulolytic potential, which could be used as components of a biopreparation to accelerate decomposition of postharvest leftovers in agriculture.
31050258	13	88	theme	biopreparation	2601:2614	arg1	components					2585:2594	components	2585:2594	components of a biopreparation	2585:2614	In this study, we were looking for the bacteria isolated from the natural environment with the high cellulolytic potential, which could be used as components of a biopreparation to accelerate decomposition of postharvest leftovers in agriculture.
31050258	3	89	theme	microorganisms	536:549	arg1	Many					508:511	Many	508:511	Many	508:511	Many of cellulose-degrading microorganisms have been isolated so far and characterized in various regions of the world.
31050258	3	89	theme	microorganisms	536:549	arg1	microorganisms					536:549	cellulose-degrading microorganisms	516:549	cellulose-degrading microorganisms	516:549	Many of cellulose-degrading microorganisms have been isolated so far and characterized in various regions of the world.
31050258	4	90	used	used	767:770	arg2	components					775:784	components	775:784	components of a biopreparation	775:804	In this study, we were looking for the bacteria isolated from the natural environment with the high cellulolytic potential, which could be used as components of a biopreparation to accelerate decomposition of postharvest leftovers in agriculture.
31050258	4	90	used	used	767:770	arg2	potential					741:749	the high cellulolytic potential	719:749	the high cellulolytic potential	719:749	In this study, we were looking for the bacteria isolated from the natural environment with the high cellulolytic potential, which could be used as components of a biopreparation to accelerate decomposition of postharvest leftovers in agriculture.
31050258	4	91	theme	biopreparation	791:804	arg1	potential					741:749	the high cellulolytic potential	719:749	the high cellulolytic potential	719:749	In this study, we were looking for the bacteria isolated from the natural environment with the high cellulolytic potential, which could be used as components of a biopreparation to accelerate decomposition of postharvest leftovers in agriculture.
31050258	4	91	theme	biopreparation	791:804	arg1	components					775:784	components	775:784	components of a biopreparation	775:804	In this study, we were looking for the bacteria isolated from the natural environment with the high cellulolytic potential, which could be used as components of a biopreparation to accelerate decomposition of postharvest leftovers in agriculture.
31050258	1	92	theme	enzymes	189:195	arg1	source					168:173	a rich source	161:173	a rich source of commercial enzymes, including amylases, proteases, cellulases, glucose isomerase, and pullulanase	161:274	Bacteria from the genus Bacillus are a rich source of commercial enzymes, including amylases, proteases, cellulases, glucose isomerase, and pullulanase.
31050258	1	92	theme	enzymes	189:195	arg1	Bacteria					124:131	Bacteria	124:131	Bacteria from the genus Bacillus	124:155	Bacteria from the genus Bacillus are a rich source of commercial enzymes, including amylases, proteases, cellulases, glucose isomerase, and pullulanase.
31050258	16	93	theme	process	3092:3098	arg1	temperature					3072:3082	optimal temperature	3064:3082	optimal temperature of this process	3064:3098	Optimization of biosynthesis of the cellulose-degrading enzymes indicated that optimal temperature of this process fluctuated in the range of 21–42°C (depending on the strain and carbon source).
31050258	8	94	theme	production	1700:1709	arg1	process					1678:1684	a simple, cost-efficient process	1653:1684	a simple, cost-efficient process of cellulases production	1653:1709	The highest cellulolytic activity was observed for the isolates designed as 4/7 (identified as Bacillus subtilis) and 4/18 (identified as Bacillus licheniformis) in a temperature of 32°C. With the use of a desirability function methodology, the optimal medium composition to achieve a simple, cost-efficient process of cellulases production was developed for both strains.
31050258	16	95	theme	biosynthesis	3001:3012	arg1	Optimization					2985:2996	Optimization	2985:2996	Optimization of biosynthesis of the cellulose-degrading enzymes	2985:3047	Optimization of biosynthesis of the cellulose-degrading enzymes indicated that optimal temperature of this process fluctuated in the range of 21–42°C (depending on the strain and carbon source).
31050258	11	96	theme	synthesis	2289:2297	arg1	level					2273:2277	the level	2269:2277	the level of enzyme synthesis	2269:2297	Cellulases account for 15% of the global market of industrial enzymes; thus, new microorganisms producing cellulases in a higher concentration and new ingredients, which can enhance the level of enzyme synthesis, are still needed.
31050258	5	97	attach	isolated	906:913	arg2	strains					893:899	The 214 bacterial strains	875:899	The 214 bacterial strains	875:899	The 214 bacterial strains were isolated from environmental samples rich in cellulose and their ability to synthesize cellulases were examined using the diffusion method.
31050258	5	97	attach	isolated	906:913	arg1	samples					934:940	environmental samples	920:940	environmental samples rich in cellulose	920:958	The 214 bacterial strains were isolated from environmental samples rich in cellulose and their ability to synthesize cellulases were examined using the diffusion method.
31050258	12	98	theme	cellulose-degrading	2326:2344	arg1	microorganisms					2346:2359	cellulose-degrading microorganisms	2326:2359	cellulose-degrading microorganisms	2326:2359	Many of cellulose-degrading microorganisms have been isolated so far and characterized in various regions of the world.
31050258	4	99	theme	leftovers	849:857	arg1	decomposition					820:832	decomposition	820:832	decomposition of postharvest leftovers	820:857	In this study, we were looking for the bacteria isolated from the natural environment with the high cellulolytic potential, which could be used as components of a biopreparation to accelerate decomposition of postharvest leftovers in agriculture.
31050258	7	100	theme	carbon	1354:1359	arg1	source					1361:1366	the strain and carbon source	1339:1366	source	1361:1366	Optimization of biosynthesis of the cellulose-degrading enzymes indicated that optimal temperature of this process fluctuated in the range of 21-42°C (depending on the strain and carbon source).
31050258	9	101	contain	have	1830:1833	arg2	potential					1857:1865	potential	1857:1865	potential for commercial applications	1857:1893	These experiments show that microorganisms isolated from natural environmental samples have unique properties and potential for commercial applications (e.g. for biopreparations production).
31050258	9	101	contain	have	1830:1833	arg1	microorganisms					1771:1784	microorganisms	1771:1784	microorganisms isolated from natural environmental samples	1771:1828	These experiments show that microorganisms isolated from natural environmental samples have unique properties and potential for commercial applications (e.g. for biopreparations production).
31050258	9	101	contain	have	1830:1833	arg2	properties					1842:1851	unique properties	1835:1851	unique properties	1835:1851	These experiments show that microorganisms isolated from natural environmental samples have unique properties and potential for commercial applications (e.g. for biopreparations production).
31050258	5	102	theme	bacterial	883:891	arg1	strains					893:899	The 214 bacterial strains	875:899	The 214 bacterial strains	875:899	The 214 bacterial strains were isolated from environmental samples rich in cellulose and their ability to synthesize cellulases were examined using the diffusion method.
31050258	7	103	theme	strain	1343:1348	arg1	source					1361:1366	the strain and carbon source	1339:1366	source	1361:1366	Optimization of biosynthesis of the cellulose-degrading enzymes indicated that optimal temperature of this process fluctuated in the range of 21-42°C (depending on the strain and carbon source).
31050258	8	104	with	temperature	1537:1547	arg1	use					1567:1569	the use	1563:1569	the use of a desirability function methodology	1563:1608	The highest cellulolytic activity was observed for the isolates designed as 4/7 (identified as Bacillus subtilis) and 4/18 (identified as Bacillus licheniformis) in a temperature of 32°C. With the use of a desirability function methodology, the optimal medium composition to achieve a simple, cost-efficient process of cellulases production was developed for both strains.
31050258	15	105	theme	clearing	2903:2910	arg1	zone					2912:2915	clearing zone	2903:2915	clearing zone	2903:2915	Six strains, which have the highest diameter of clearing zone both for biomass and supernatant, were selected for identification.
31050258	14	106	theme	environmental	2730:2742	arg1	samples					2744:2750	environmental samples	2730:2750	environmental samples rich in cellulose	2730:2768	The 214 bacterial strains were isolated from environmental samples rich in cellulose and their ability to synthesize cellulases were examined using the diffusion method.
31050258	18	107	theme	unique	3645:3650	arg1	properties					3652:3661	unique properties	3645:3661	unique properties	3645:3661	These experiments show that microorganisms isolated from natural environmental samples have unique properties and potential for commercial applications (e.g. for biopreparations production).
31050258	10	108	theme	commercial	1988:1997	arg1	cellulases					2039:2048	cellulases	2039:2048	cellulases	2039:2048	Bacteria from the genus Bacillus are a rich source of commercial enzymes, including amylases, proteases, cellulases, glucose isomerase, and pullulanase.
31050258	10	108	theme	commercial	1988:1997	arg1	isomerase					2059:2067	glucose isomerase	2051:2067	glucose isomerase	2051:2067	Bacteria from the genus Bacillus are a rich source of commercial enzymes, including amylases, proteases, cellulases, glucose isomerase, and pullulanase.
31050258	10	108	theme	commercial	1988:1997	arg1	amylases					2018:2025	amylases	2018:2025	amylases	2018:2025	Bacteria from the genus Bacillus are a rich source of commercial enzymes, including amylases, proteases, cellulases, glucose isomerase, and pullulanase.
31050258	10	108	theme	commercial	1988:1997	arg1	proteases					2028:2036	proteases	2028:2036	proteases	2028:2036	Bacteria from the genus Bacillus are a rich source of commercial enzymes, including amylases, proteases, cellulases, glucose isomerase, and pullulanase.
31050258	10	108	theme	commercial	1988:1997	arg1	pullulanase					2074:2084	pullulanase	2074:2084	pullulanase	2074:2084	Bacteria from the genus Bacillus are a rich source of commercial enzymes, including amylases, proteases, cellulases, glucose isomerase, and pullulanase.
31050258	10	108	theme	commercial	1988:1997	arg1	enzymes					1999:2005	commercial enzymes	1988:2005	commercial enzymes	1988:2005	Bacteria from the genus Bacillus are a rich source of commercial enzymes, including amylases, proteases, cellulases, glucose isomerase, and pullulanase.
31050258	0	109	theme	Microbiota	78:87	arg1	Potential					65:73	Cellulolytic Potential	52:73	Cellulolytic Potential of Microbiota	52:87	The Influence of Temperature and Nitrogen Source on Cellulolytic Potential of Microbiota Isolated from Natural Environment.
31050258	10	110	from	genus	1952:1956	arg1	source					1978:1983	a rich source	1971:1983	a rich source of commercial enzymes, including amylases, proteases, cellulases, glucose isomerase, and pullulanase	1971:2084	Bacteria from the genus Bacillus are a rich source of commercial enzymes, including amylases, proteases, cellulases, glucose isomerase, and pullulanase.
31050258	10	110	from	genus	1952:1956	arg1	Bacteria					1934:1941	Bacteria	1934:1941	Bacteria from the genus Bacillus	1934:1965	Bacteria from the genus Bacillus are a rich source of commercial enzymes, including amylases, proteases, cellulases, glucose isomerase, and pullulanase.
31050258	17	111	dep	composition	3440:3450	arg1	achieve					3455:3461	achieve	3455:3461	to achieve a simple, cost-efficient process of cellulases production	3452:3519	The highest cellulolytic activity was observed for the isolates designed as 4/7 (identified as Bacillus subtilis) and 4/18 (identified as Bacillus licheniformis) in a temperature of 32°C. With the use of a desirability function methodology, the optimal medium composition to achieve a simple, cost-efficient process of cellulases production was developed for both strains.
31050258	0	112	from	Influence	4:12	arg1	Potential					65:73	Cellulolytic Potential	52:73	Cellulolytic Potential of Microbiota	52:87	The Influence of Temperature and Nitrogen Source on Cellulolytic Potential of Microbiota Isolated from Natural Environment.
31050258	16	113	theme	cellulose-degrading	3021:3039	arg1	enzymes					3041:3047	the cellulose-degrading enzymes	3017:3047	the cellulose-degrading enzymes	3017:3047	Optimization of biosynthesis of the cellulose-degrading enzymes indicated that optimal temperature of this process fluctuated in the range of 21–42°C (depending on the strain and carbon source).
31050258	16	114	theme	strain	3153:3158	arg1	source					3171:3176	the strain and carbon source	3149:3176	source	3171:3176	Optimization of biosynthesis of the cellulose-degrading enzymes indicated that optimal temperature of this process fluctuated in the range of 21–42°C (depending on the strain and carbon source).
31050258	10	115	dep	genus	1952:1956	arg1	Bacillus					1958:1965	the genus Bacillus	1948:1965	the genus Bacillus	1948:1965	Bacteria from the genus Bacillus are a rich source of commercial enzymes, including amylases, proteases, cellulases, glucose isomerase, and pullulanase.
31050258	17	116	theme	production	3510:3519	arg1	process					3488:3494	a simple, cost-efficient process	3463:3494	a simple, cost-efficient process of cellulases production	3463:3519	The highest cellulolytic activity was observed for the isolates designed as 4/7 (identified as Bacillus subtilis) and 4/18 (identified as Bacillus licheniformis) in a temperature of 32°C. With the use of a desirability function methodology, the optimal medium composition to achieve a simple, cost-efficient process of cellulases production was developed for both strains.
31050258	17	117	theme	highest	3184:3190	arg1	activity					3205:3212	The highest cellulolytic activity	3180:3212	The highest cellulolytic activity	3180:3212	The highest cellulolytic activity was observed for the isolates designed as 4/7 (identified as Bacillus subtilis) and 4/18 (identified as Bacillus licheniformis) in a temperature of 32°C. With the use of a desirability function methodology, the optimal medium composition to achieve a simple, cost-efficient process of cellulases production was developed for both strains.
31050258	16	118	theme	carbon	3164:3169	arg1	source					3171:3176	the strain and carbon source	3149:3176	source	3171:3176	Optimization of biosynthesis of the cellulose-degrading enzymes indicated that optimal temperature of this process fluctuated in the range of 21–42°C (depending on the strain and carbon source).
31050258	16	119	theme	21–42°C	3127:3133	arg1	range					3118:3122	the range	3114:3122	the range of 21–42°C	3114:3133	Optimization of biosynthesis of the cellulose-degrading enzymes indicated that optimal temperature of this process fluctuated in the range of 21–42°C (depending on the strain and carbon source).
31050258	7	120	theme	21-42°C	1317:1323	arg1	range					1308:1312	the range	1304:1312	the range of 21-42°C	1304:1323	Optimization of biosynthesis of the cellulose-degrading enzymes indicated that optimal temperature of this process fluctuated in the range of 21-42°C (depending on the strain and carbon source).
31050258	2	121	theme	enzyme	472:477	arg1	synthesis					479:487	enzyme synthesis	472:487	enzyme synthesis	472:487	Cellulases account for 15% of the global market of industrial enzymes; thus, new microorganisms producing cellulases in a higher concentration and new ingredients, which can enhance the level of enzyme synthesis, are still needed.
31050258	4	122	theme	high	723:726	arg1	potential					741:749	the high cellulolytic potential	719:749	the high cellulolytic potential	719:749	In this study, we were looking for the bacteria isolated from the natural environment with the high cellulolytic potential, which could be used as components of a biopreparation to accelerate decomposition of postharvest leftovers in agriculture.
31050258	4	122	theme	high	723:726	arg1	components					775:784	components	775:784	components of a biopreparation	775:804	In this study, we were looking for the bacteria isolated from the natural environment with the high cellulolytic potential, which could be used as components of a biopreparation to accelerate decomposition of postharvest leftovers in agriculture.
31050258	2	123	theme	new	354:356	arg1	microorganisms					358:371	new microorganisms	354:371	new microorganisms producing cellulases in a higher concentration and new ingredients, which can enhance the level of enzyme synthesis,	354:488	Cellulases account for 15% of the global market of industrial enzymes; thus, new microorganisms producing cellulases in a higher concentration and new ingredients, which can enhance the level of enzyme synthesis, are still needed.
31050258	13	124	theme	cellulolytic	2538:2549	arg1	potential					2551:2559	the high cellulolytic potential	2529:2559	the high cellulolytic potential	2529:2559	In this study, we were looking for the bacteria isolated from the natural environment with the high cellulolytic potential, which could be used as components of a biopreparation to accelerate decomposition of postharvest leftovers in agriculture.
31050258	13	124	theme	cellulolytic	2538:2549	arg1	components					2585:2594	components	2585:2594	components of a biopreparation	2585:2614	In this study, we were looking for the bacteria isolated from the natural environment with the high cellulolytic potential, which could be used as components of a biopreparation to accelerate decomposition of postharvest leftovers in agriculture.
31050258	17	125	dep	simple	3465:3470	arg1	cost-efficient					3473:3486	cost-efficient	3473:3486	cost-efficient	3473:3486	The highest cellulolytic activity was observed for the isolates designed as 4/7 (identified as Bacillus subtilis) and 4/18 (identified as Bacillus licheniformis) in a temperature of 32°C. With the use of a desirability function methodology, the optimal medium composition to achieve a simple, cost-efficient process of cellulases production was developed for both strains.
31050258	11	126	theme	enzymes	2149:2155	arg1	market					2128:2133	the global market	2117:2133	the global market of industrial enzymes	2117:2155	Cellulases account for 15% of the global market of industrial enzymes; thus, new microorganisms producing cellulases in a higher concentration and new ingredients, which can enhance the level of enzyme synthesis, are still needed.
31050258	16	127	theme	enzymes	3041:3047	arg1	biosynthesis					3001:3012	biosynthesis	3001:3012	biosynthesis of the cellulose-degrading enzymes	3001:3047	Optimization of biosynthesis of the cellulose-degrading enzymes indicated that optimal temperature of this process fluctuated in the range of 21–42°C (depending on the strain and carbon source).
31050258	0	128	theme	Temperature	17:27	arg1	Influence					4:12	The Influence	0:12	The Influence of Temperature and Nitrogen Source on Cellulolytic Potential of Microbiota	0:87	The Influence of Temperature and Nitrogen Source on Cellulolytic Potential of Microbiota Isolated from Natural Environment.
31050258	14	129	theme	bacterial	2693:2701	arg1	strains					2703:2709	The 214 bacterial strains	2685:2709	The 214 bacterial strains	2685:2709	The 214 bacterial strains were isolated from environmental samples rich in cellulose and their ability to synthesize cellulases were examined using the diffusion method.
31050258	2	130	theme	higher	399:404	arg1	concentration					406:418	a higher concentration	397:418	a higher concentration	397:418	Cellulases account for 15% of the global market of industrial enzymes; thus, new microorganisms producing cellulases in a higher concentration and new ingredients, which can enhance the level of enzyme synthesis, are still needed.
31050258	4	131	theme	natural	694:700	arg1	environment					702:712	the natural environment	690:712	the natural environment	690:712	In this study, we were looking for the bacteria isolated from the natural environment with the high cellulolytic potential, which could be used as components of a biopreparation to accelerate decomposition of postharvest leftovers in agriculture.
31050258	0	132	theme	Nitrogen	33:40	arg1	Source					42:47	Nitrogen Source	33:47	Nitrogen Source	33:47	The Influence of Temperature and Nitrogen Source on Cellulolytic Potential of Microbiota Isolated from Natural Environment.
31050258	8	133	dep	4/7	1446:1448	arg1	identified					1451:1460	identified	1451:1460	identified as Bacillus subtilis	1451:1481	The highest cellulolytic activity was observed for the isolates designed as 4/7 (identified as Bacillus subtilis) and 4/18 (identified as Bacillus licheniformis) in a temperature of 32°C. With the use of a desirability function methodology, the optimal medium composition to achieve a simple, cost-efficient process of cellulases production was developed for both strains.
31050258	7	134	theme	process	1282:1288	arg1	temperature					1262:1272	optimal temperature	1254:1272	optimal temperature of this process	1254:1288	Optimization of biosynthesis of the cellulose-degrading enzymes indicated that optimal temperature of this process fluctuated in the range of 21-42°C (depending on the strain and carbon source).
31050258	9	135	theme	environmental	1808:1820	arg1	samples					1822:1828	natural environmental samples	1800:1828	natural environmental samples	1800:1828	These experiments show that microorganisms isolated from natural environmental samples have unique properties and potential for commercial applications (e.g. for biopreparations production).
31050258	11	136	theme	higher	2209:2214	arg1	concentration					2216:2228	a higher concentration	2207:2228	a higher concentration	2207:2228	Cellulases account for 15% of the global market of industrial enzymes; thus, new microorganisms producing cellulases in a higher concentration and new ingredients, which can enhance the level of enzyme synthesis, are still needed.
31050258	6	137	theme	zone	1102:1105	arg1	diameter					1081:1088	the highest diameter	1069:1088	the highest diameter of clearing zone both for biomass and supernatant	1069:1138	Six strains, which have the highest diameter of clearing zone both for biomass and supernatant, were selected for identification.
31050258	8	138	theme	32°C.	1552:1556	arg1	temperature					1537:1547	a temperature	1535:1547	a temperature of 32°C. With the use of a desirability function methodology	1535:1608	The highest cellulolytic activity was observed for the isolates designed as 4/7 (identified as Bacillus subtilis) and 4/18 (identified as Bacillus licheniformis) in a temperature of 32°C. With the use of a desirability function methodology, the optimal medium composition to achieve a simple, cost-efficient process of cellulases production was developed for both strains.
31050258	17	139	theme	simple	3465:3470	arg1	process					3488:3494	a simple, cost-efficient process	3463:3494	a simple, cost-efficient process of cellulases production	3463:3519	The highest cellulolytic activity was observed for the isolates designed as 4/7 (identified as Bacillus subtilis) and 4/18 (identified as Bacillus licheniformis) in a temperature of 32°C. With the use of a desirability function methodology, the optimal medium composition to achieve a simple, cost-efficient process of cellulases production was developed for both strains.
31050258	8	140	theme	highest	1374:1380	arg1	activity					1395:1402	The highest cellulolytic activity	1370:1402	The highest cellulolytic activity	1370:1402	The highest cellulolytic activity was observed for the isolates designed as 4/7 (identified as Bacillus subtilis) and 4/18 (identified as Bacillus licheniformis) in a temperature of 32°C. With the use of a desirability function methodology, the optimal medium composition to achieve a simple, cost-efficient process of cellulases production was developed for both strains.
31050258	17	141	theme	desirability	3386:3397	arg1	methodology					3408:3418	a desirability function methodology	3384:3418	a desirability function methodology	3384:3418	The highest cellulolytic activity was observed for the isolates designed as 4/7 (identified as Bacillus subtilis) and 4/18 (identified as Bacillus licheniformis) in a temperature of 32°C. With the use of a desirability function methodology, the optimal medium composition to achieve a simple, cost-efficient process of cellulases production was developed for both strains.
31050258	2	142	theme	global	311:316	arg1	market					318:323	the global market	307:323	the global market of industrial enzymes	307:345	Cellulases account for 15% of the global market of industrial enzymes; thus, new microorganisms producing cellulases in a higher concentration and new ingredients, which can enhance the level of enzyme synthesis, are still needed.
31050258	15	143	contain	have	2874:2877	arg1	strains					2859:2865	Six strains	2855:2865	Six strains	2855:2865	Six strains, which have the highest diameter of clearing zone both for biomass and supernatant, were selected for identification.
31050258	15	143	contain	have	2874:2877	arg2	diameter					2891:2898	the highest diameter	2879:2898	the highest diameter of clearing zone both for biomass and supernatant	2879:2948	Six strains, which have the highest diameter of clearing zone both for biomass and supernatant, were selected for identification.
31050258	7	144	theme	optimal	1254:1260	arg1	temperature					1262:1272	optimal temperature	1254:1272	optimal temperature of this process	1254:1288	Optimization of biosynthesis of the cellulose-degrading enzymes indicated that optimal temperature of this process fluctuated in the range of 21-42°C (depending on the strain and carbon source).
31050258	10	145	theme	glucose	2051:2057	arg1	isomerase					2059:2067	glucose isomerase	2051:2067	glucose isomerase	2051:2067	Bacteria from the genus Bacillus are a rich source of commercial enzymes, including amylases, proteases, cellulases, glucose isomerase, and pullulanase.
31050258	11	146	from	cellulases	2193:2202	arg1	concentration					2216:2228	a higher concentration	2207:2228	a higher concentration	2207:2228	Cellulases account for 15% of the global market of industrial enzymes; thus, new microorganisms producing cellulases in a higher concentration and new ingredients, which can enhance the level of enzyme synthesis, are still needed.
31050258	11	146	from	cellulases	2193:2202	arg1	ingredients					2238:2248	new ingredients	2234:2248	new ingredients	2234:2248	Cellulases account for 15% of the global market of industrial enzymes; thus, new microorganisms producing cellulases in a higher concentration and new ingredients, which can enhance the level of enzyme synthesis, are still needed.
31050258	17	147	theme	methodology	3408:3418	arg1	use					3377:3379	the use	3373:3379	the use of a desirability function methodology	3373:3418	The highest cellulolytic activity was observed for the isolates designed as 4/7 (identified as Bacillus subtilis) and 4/18 (identified as Bacillus licheniformis) in a temperature of 32°C. With the use of a desirability function methodology, the optimal medium composition to achieve a simple, cost-efficient process of cellulases production was developed for both strains.
31050258	8	148	theme	medium	1623:1628	arg1	composition					1630:1640	the optimal medium composition	1611:1640	the optimal medium composition to achieve a simple, cost-efficient process of cellulases production	1611:1709	The highest cellulolytic activity was observed for the isolates designed as 4/7 (identified as Bacillus subtilis) and 4/18 (identified as Bacillus licheniformis) in a temperature of 32°C. With the use of a desirability function methodology, the optimal medium composition to achieve a simple, cost-efficient process of cellulases production was developed for both strains.
31050258	6	149	theme	highest	1073:1079	arg1	diameter					1081:1088	the highest diameter	1069:1088	the highest diameter of clearing zone both for biomass and supernatant	1069:1138	Six strains, which have the highest diameter of clearing zone both for biomass and supernatant, were selected for identification.
31050258	11	150	theme	enzyme	2282:2287	arg1	synthesis					2289:2297	enzyme synthesis	2282:2297	enzyme synthesis	2282:2297	Cellulases account for 15% of the global market of industrial enzymes; thus, new microorganisms producing cellulases in a higher concentration and new ingredients, which can enhance the level of enzyme synthesis, are still needed.
31050258	2	151	theme	enzymes	339:345	arg1	market					318:323	the global market	307:323	the global market of industrial enzymes	307:345	Cellulases account for 15% of the global market of industrial enzymes; thus, new microorganisms producing cellulases in a higher concentration and new ingredients, which can enhance the level of enzyme synthesis, are still needed.
31050258	8	152	dep	4/18	1488:1491	arg1	identified					1494:1503	identified	1494:1503	identified as Bacillus licheniformis	1494:1529	The highest cellulolytic activity was observed for the isolates designed as 4/7 (identified as Bacillus subtilis) and 4/18 (identified as Bacillus licheniformis) in a temperature of 32°C. With the use of a desirability function methodology, the optimal medium composition to achieve a simple, cost-efficient process of cellulases production was developed for both strains.
31822562	6	0	theme	activity	895:902	arg1	Parameters					812:821	Parameters	812:821	Parameters of lipid metabolism, glucose homeostasis, and mitochondrial respiratory activity	812:902	Parameters of lipid metabolism, glucose homeostasis, and mitochondrial respiratory activity were measured in vivo and ex vivo Compared with NOD TLR4+/+ mice, NOD TLR4-/- animals showed lower muscle mass of the small intestine, higher abundance of Bacteroidetes, and lower Firmicutes in the large intestine, along with lower levels of circulating short-chain fatty acids (SCFA).
31822562	9	1	theme	circulating	1618:1628	arg1	SCFA					1630:1633	circulating SCFA	1618:1633	circulating SCFA	1618:1633	Distinct alterations of gut morphology and microbiota composition associated with reduction of circulating SCFA may contribute to metabolic disorders promoting the progression of insulin-deficient diabetes/T1D development.
31822562	4	2	theme	intestinal	528:537	arg1	functions					539:547	altered intestinal functions	520:547	altered intestinal functions	520:547	We hypothesized that altered intestinal functions contribute to metabolic alterations, which favor accelerated diabetes development in TLR4-deficient (TLR4-/-) NOD mice.
31822562	6	3	theme	mitochondrial	869:881	arg1	activity					895:902	mitochondrial respiratory activity	869:902	mitochondrial respiratory activity	869:902	Parameters of lipid metabolism, glucose homeostasis, and mitochondrial respiratory activity were measured in vivo and ex vivo Compared with NOD TLR4+/+ mice, NOD TLR4-/- animals showed lower muscle mass of the small intestine, higher abundance of Bacteroidetes, and lower Firmicutes in the large intestine, along with lower levels of circulating short-chain fatty acids (SCFA).
31822562	6	4	theme	lower	1130:1134	arg1	levels					1136:1141	lower levels	1130:1141	lower levels of circulating short-chain fatty acids (SCFA)	1130:1187	Parameters of lipid metabolism, glucose homeostasis, and mitochondrial respiratory activity were measured in vivo and ex vivo Compared with NOD TLR4+/+ mice, NOD TLR4-/- animals showed lower muscle mass of the small intestine, higher abundance of Bacteroidetes, and lower Firmicutes in the large intestine, along with lower levels of circulating short-chain fatty acids (SCFA).
31822562	1	5	theme	underlying	236:245	arg1	mechanisms					247:256	the underlying mechanisms	232:256	the underlying mechanisms	232:256	The rising prevalence of type 1 diabetes (T1D) over the past decades has been linked to lifestyle changes, but the underlying mechanisms are largely unknown.
31822562	1	5	theme	underlying	236:245	arg1	unknown					270:276	unknown	270:276	unknown	270:276	The rising prevalence of type 1 diabetes (T1D) over the past decades has been linked to lifestyle changes, but the underlying mechanisms are largely unknown.
31822562	6	6	theme	glucose	844:850	arg1	homeostasis					852:862	glucose homeostasis	844:862	glucose homeostasis	844:862	Parameters of lipid metabolism, glucose homeostasis, and mitochondrial respiratory activity were measured in vivo and ex vivo Compared with NOD TLR4+/+ mice, NOD TLR4-/- animals showed lower muscle mass of the small intestine, higher abundance of Bacteroidetes, and lower Firmicutes in the large intestine, along with lower levels of circulating short-chain fatty acids (SCFA).
31822562	7	7	theme	diabetes	1338:1345	arg1	onset					1329:1333	the onset	1325:1333	the onset of diabetes	1325:1345	These changes are associated with higher body weight, hyperlipidemia, and severe insulin and glucose intolerance, all occurring before the onset of diabetes.
31822562	8	8	theme	insulin	1374:1380	arg1	abnormalities					1401:1413	insulin resistance-related abnormalities	1374:1413	insulin resistance-related abnormalities	1374:1413	These mice also exhibited insulin resistance-related abnormalities of energy metabolism, such as lower total respiratory exchange rates and higher hepatic oxidative capacity.
31822562	8	8	theme	insulin	1374:1380	arg1	capacity					1513:1520	lower total respiratory exchange rates and higher hepatic oxidative capacity	1445:1520	lower total respiratory exchange rates and higher hepatic oxidative capacity	1445:1520	These mice also exhibited insulin resistance-related abnormalities of energy metabolism, such as lower total respiratory exchange rates and higher hepatic oxidative capacity.
31822562	4	9	theme	diabetes	610:617	arg1	development					619:629	accelerated diabetes development	598:629	accelerated diabetes development	598:629	We hypothesized that altered intestinal functions contribute to metabolic alterations, which favor accelerated diabetes development in TLR4-deficient (TLR4-/-) NOD mice.
31822562	5	10	theme	normoglycemic	686:698	arg1	TLR4+/+					725:731	70-90-day-old normoglycemic (prediabetic) female NOD TLR4+/+ and NOD TLR4-/- mice	672:752	TLR4+/+	725:731	In 70-90-day-old normoglycemic (prediabetic) female NOD TLR4+/+ and NOD TLR4-/- mice, gut morphology and microbiome composition were analyzed.
31822562	5	11	theme	microbiome	774:783	arg1	composition					785:795	microbiome composition	774:795	microbiome composition	774:795	In 70-90-day-old normoglycemic (prediabetic) female NOD TLR4+/+ and NOD TLR4-/- mice, gut morphology and microbiome composition were analyzed.
31822562	9	12	theme	diabetes/T1D	1720:1731	arg1	development					1733:1743	insulin-deficient diabetes/T1D development	1702:1743	insulin-deficient diabetes/T1D development	1702:1743	Distinct alterations of gut morphology and microbiota composition associated with reduction of circulating SCFA may contribute to metabolic disorders promoting the progression of insulin-deficient diabetes/T1D development.
31822562	6	13	theme	acids	1176:1180	arg1	levels					1136:1141	lower levels	1130:1141	lower levels of circulating short-chain fatty acids (SCFA)	1130:1187	Parameters of lipid metabolism, glucose homeostasis, and mitochondrial respiratory activity were measured in vivo and ex vivo Compared with NOD TLR4+/+ mice, NOD TLR4-/- animals showed lower muscle mass of the small intestine, higher abundance of Bacteroidetes, and lower Firmicutes in the large intestine, along with lower levels of circulating short-chain fatty acids (SCFA).
31822562	1	14	theme	rising	125:130	arg1	prevalence					132:141	The rising prevalence	121:141	The rising prevalence of type 1 diabetes (T1D) over the past decades	121:188	The rising prevalence of type 1 diabetes (T1D) over the past decades has been linked to lifestyle changes, but the underlying mechanisms are largely unknown.
31822562	8	15	theme	energy	1418:1423	arg1	metabolism					1425:1434	energy metabolism	1418:1434	energy metabolism	1418:1434	These mice also exhibited insulin resistance-related abnormalities of energy metabolism, such as lower total respiratory exchange rates and higher hepatic oxidative capacity.
31822562	6	16	theme	short-chain	1158:1168	arg1	acids					1176:1180	circulating short-chain fatty acids	1146:1180	circulating short-chain fatty acids (SCFA)	1146:1187	Parameters of lipid metabolism, glucose homeostasis, and mitochondrial respiratory activity were measured in vivo and ex vivo Compared with NOD TLR4+/+ mice, NOD TLR4-/- animals showed lower muscle mass of the small intestine, higher abundance of Bacteroidetes, and lower Firmicutes in the large intestine, along with lower levels of circulating short-chain fatty acids (SCFA).
31822562	6	16	theme	short-chain	1158:1168	arg1	SCFA					1183:1186	SCFA	1183:1186	SCFA	1183:1186	Parameters of lipid metabolism, glucose homeostasis, and mitochondrial respiratory activity were measured in vivo and ex vivo Compared with NOD TLR4+/+ mice, NOD TLR4-/- animals showed lower muscle mass of the small intestine, higher abundance of Bacteroidetes, and lower Firmicutes in the large intestine, along with lower levels of circulating short-chain fatty acids (SCFA).
31822562	0	17	theme	accelerated	67:77	arg1	onset					88:92	accelerated diabetes onset	67:92	accelerated diabetes onset	67:92	Distinct alterations of gut morphology and microbiota characterize accelerated diabetes onset in nonobese diabetic mice.
31822562	6	18	theme	NOD	970:972	arg1	animals					982:988	NOD TLR4-/- animals	970:988	NOD TLR4-/- animals	970:988	Parameters of lipid metabolism, glucose homeostasis, and mitochondrial respiratory activity were measured in vivo and ex vivo Compared with NOD TLR4+/+ mice, NOD TLR4-/- animals showed lower muscle mass of the small intestine, higher abundance of Bacteroidetes, and lower Firmicutes in the large intestine, along with lower levels of circulating short-chain fatty acids (SCFA).
31822562	5	19	theme	NOD	737:739	arg1	mice					749:752	70-90-day-old normoglycemic (prediabetic) female NOD TLR4+/+ and NOD TLR4-/- mice	672:752	mice	749:752	In 70-90-day-old normoglycemic (prediabetic) female NOD TLR4+/+ and NOD TLR4-/- mice, gut morphology and microbiome composition were analyzed.
31822562	8	20	theme	total	1451:1455	arg1	rates					1478:1482	total respiratory exchange rates	1451:1482	total respiratory exchange rates	1451:1482	These mice also exhibited insulin resistance-related abnormalities of energy metabolism, such as lower total respiratory exchange rates and higher hepatic oxidative capacity.
31822562	0	21	theme	nonobese	97:104	arg1	mice					115:118	nonobese diabetic mice	97:118	nonobese diabetic mice	97:118	Distinct alterations of gut morphology and microbiota characterize accelerated diabetes onset in nonobese diabetic mice.
31822562	6	22	theme	NOD	952:954	arg1	mice					964:967	NOD TLR4+/+ mice	952:967	NOD TLR4+/+ mice	952:967	Parameters of lipid metabolism, glucose homeostasis, and mitochondrial respiratory activity were measured in vivo and ex vivo Compared with NOD TLR4+/+ mice, NOD TLR4-/- animals showed lower muscle mass of the small intestine, higher abundance of Bacteroidetes, and lower Firmicutes in the large intestine, along with lower levels of circulating short-chain fatty acids (SCFA).
31822562	8	23	theme	exchange	1469:1476	arg1	rates					1478:1482	total respiratory exchange rates	1451:1482	total respiratory exchange rates	1451:1482	These mice also exhibited insulin resistance-related abnormalities of energy metabolism, such as lower total respiratory exchange rates and higher hepatic oxidative capacity.
31822562	5	24	theme	female	714:719	arg1	TLR4+/+					725:731	70-90-day-old normoglycemic (prediabetic) female NOD TLR4+/+ and NOD TLR4-/- mice	672:752	TLR4+/+	725:731	In 70-90-day-old normoglycemic (prediabetic) female NOD TLR4+/+ and NOD TLR4-/- mice, gut morphology and microbiome composition were analyzed.
31822562	6	25	theme	intestine	1028:1036	arg1	mass					1010:1013	lower muscle mass	997:1013	lower muscle mass of the small intestine	997:1036	Parameters of lipid metabolism, glucose homeostasis, and mitochondrial respiratory activity were measured in vivo and ex vivo Compared with NOD TLR4+/+ mice, NOD TLR4-/- animals showed lower muscle mass of the small intestine, higher abundance of Bacteroidetes, and lower Firmicutes in the large intestine, along with lower levels of circulating short-chain fatty acids (SCFA).
31822562	6	25	theme	intestine	1028:1036	arg1	abundance					1046:1054	higher abundance	1039:1054	higher abundance of Bacteroidetes	1039:1071	Parameters of lipid metabolism, glucose homeostasis, and mitochondrial respiratory activity were measured in vivo and ex vivo Compared with NOD TLR4+/+ mice, NOD TLR4-/- animals showed lower muscle mass of the small intestine, higher abundance of Bacteroidetes, and lower Firmicutes in the large intestine, along with lower levels of circulating short-chain fatty acids (SCFA).
31822562	6	25	theme	intestine	1028:1036	arg1	Firmicutes					1084:1093	lower Firmicutes	1078:1093	lower Firmicutes in the large intestine	1078:1116	Parameters of lipid metabolism, glucose homeostasis, and mitochondrial respiratory activity were measured in vivo and ex vivo Compared with NOD TLR4+/+ mice, NOD TLR4-/- animals showed lower muscle mass of the small intestine, higher abundance of Bacteroidetes, and lower Firmicutes in the large intestine, along with lower levels of circulating short-chain fatty acids (SCFA).
31822562	0	26	theme	diabetes	79:86	arg1	onset					88:92	accelerated diabetes onset	67:92	accelerated diabetes onset	67:92	Distinct alterations of gut morphology and microbiota characterize accelerated diabetes onset in nonobese diabetic mice.
31822562	0	27	theme	Distinct	0:7	arg1	alterations					9:19	Distinct alterations	0:19	Distinct alterations of gut morphology and microbiota	0:52	Distinct alterations of gut morphology and microbiota characterize accelerated diabetes onset in nonobese diabetic mice.
31822562	8	28	theme	hepatic	1495:1501	arg1	capacity					1513:1520	lower total respiratory exchange rates and higher hepatic oxidative capacity	1445:1520	lower total respiratory exchange rates and higher hepatic oxidative capacity	1445:1520	These mice also exhibited insulin resistance-related abnormalities of energy metabolism, such as lower total respiratory exchange rates and higher hepatic oxidative capacity.
31822562	9	29	theme	gut	1547:1549	arg1	morphology					1551:1560	gut morphology	1547:1560	gut morphology	1547:1560	Distinct alterations of gut morphology and microbiota composition associated with reduction of circulating SCFA may contribute to metabolic disorders promoting the progression of insulin-deficient diabetes/T1D development.
31822562	6	30	theme	muscle	1003:1008	arg1	mass					1010:1013	lower muscle mass	997:1013	lower muscle mass of the small intestine	997:1036	Parameters of lipid metabolism, glucose homeostasis, and mitochondrial respiratory activity were measured in vivo and ex vivo Compared with NOD TLR4+/+ mice, NOD TLR4-/- animals showed lower muscle mass of the small intestine, higher abundance of Bacteroidetes, and lower Firmicutes in the large intestine, along with lower levels of circulating short-chain fatty acids (SCFA).
31822562	0	31	theme	morphology	28:37	arg1	alterations					9:19	Distinct alterations	0:19	Distinct alterations of gut morphology and microbiota	0:52	Distinct alterations of gut morphology and microbiota characterize accelerated diabetes onset in nonobese diabetic mice.
31822562	6	32	theme	lipid	826:830	arg1	metabolism					832:841	lipid metabolism	826:841	lipid metabolism	826:841	Parameters of lipid metabolism, glucose homeostasis, and mitochondrial respiratory activity were measured in vivo and ex vivo Compared with NOD TLR4+/+ mice, NOD TLR4-/- animals showed lower muscle mass of the small intestine, higher abundance of Bacteroidetes, and lower Firmicutes in the large intestine, along with lower levels of circulating short-chain fatty acids (SCFA).
31822562	6	33	theme	lower	997:1001	arg1	mass					1010:1013	lower muscle mass	997:1013	lower muscle mass of the small intestine	997:1036	Parameters of lipid metabolism, glucose homeostasis, and mitochondrial respiratory activity were measured in vivo and ex vivo Compared with NOD TLR4+/+ mice, NOD TLR4-/- animals showed lower muscle mass of the small intestine, higher abundance of Bacteroidetes, and lower Firmicutes in the large intestine, along with lower levels of circulating short-chain fatty acids (SCFA).
31822562	3	34	theme	diabetic	378:385	arg1	mice					393:396	nonobese diabetic (NOD) mice	369:396	nonobese diabetic (NOD) mice	369:396	In nonobese diabetic (NOD) mice, a model of T1D, diabetes development accelerates after deletion of the Toll-like receptor 4 (TLR4).
31822562	3	34	theme	diabetic	378:385	arg1	model					401:405	a model	399:405	a model of T1D	399:412	In nonobese diabetic (NOD) mice, a model of T1D, diabetes development accelerates after deletion of the Toll-like receptor 4 (TLR4).
31822562	5	35	theme	gut	755:757	arg1	morphology					759:768	gut morphology	755:768	gut morphology	755:768	In 70-90-day-old normoglycemic (prediabetic) female NOD TLR4+/+ and NOD TLR4-/- mice, gut morphology and microbiome composition were analyzed.
31822562	9	36	theme	composition	1577:1587	arg1	alterations					1532:1542	Distinct alterations	1523:1542	Distinct alterations of gut morphology and microbiota composition associated with reduction of circulating SCFA	1523:1633	Distinct alterations of gut morphology and microbiota composition associated with reduction of circulating SCFA may contribute to metabolic disorders promoting the progression of insulin-deficient diabetes/T1D development.
31822562	6	37	theme	lower	1078:1082	arg1	Firmicutes					1084:1093	lower Firmicutes	1078:1093	lower Firmicutes in the large intestine	1078:1116	Parameters of lipid metabolism, glucose homeostasis, and mitochondrial respiratory activity were measured in vivo and ex vivo Compared with NOD TLR4+/+ mice, NOD TLR4-/- animals showed lower muscle mass of the small intestine, higher abundance of Bacteroidetes, and lower Firmicutes in the large intestine, along with lower levels of circulating short-chain fatty acids (SCFA).
31822562	2	38	theme	gut-associated	304:317	arg1	mechanisms					319:328	gut-associated mechanisms	304:328	gut-associated mechanisms	304:328	Recent findings point to gut-associated mechanisms in the control of T1D pathogenesis.
31822562	4	39	dep	TLR4-deficient	634:647	arg1	TLR4-/-					650:656	TLR4-/-	650:656	TLR4-/-	650:656	We hypothesized that altered intestinal functions contribute to metabolic alterations, which favor accelerated diabetes development in TLR4-deficient (TLR4-/-) NOD mice.
31822562	3	40	theme	Toll-like	470:478	arg1	TLR4					492:495	TLR4	492:495	TLR4	492:495	In nonobese diabetic (NOD) mice, a model of T1D, diabetes development accelerates after deletion of the Toll-like receptor 4 (TLR4).
31822562	3	40	theme	Toll-like	470:478	arg1	receptor					480:487	Toll-like receptor 4	470:489	the Toll-like receptor 4 (TLR4)	466:496	In nonobese diabetic (NOD) mice, a model of T1D, diabetes development accelerates after deletion of the Toll-like receptor 4 (TLR4).
31822562	1	41	theme	lifestyle	209:217	arg1	changes					219:225	lifestyle changes	209:225	lifestyle changes	209:225	The rising prevalence of type 1 diabetes (T1D) over the past decades has been linked to lifestyle changes, but the underlying mechanisms are largely unknown.
31822562	6	42	theme	respiratory	883:893	arg1	activity					895:902	mitochondrial respiratory activity	869:902	mitochondrial respiratory activity	869:902	Parameters of lipid metabolism, glucose homeostasis, and mitochondrial respiratory activity were measured in vivo and ex vivo Compared with NOD TLR4+/+ mice, NOD TLR4-/- animals showed lower muscle mass of the small intestine, higher abundance of Bacteroidetes, and lower Firmicutes in the large intestine, along with lower levels of circulating short-chain fatty acids (SCFA).
31822562	9	43	theme	SCFA	1630:1633	arg1	reduction					1605:1613	reduction	1605:1613	reduction of circulating SCFA	1605:1633	Distinct alterations of gut morphology and microbiota composition associated with reduction of circulating SCFA may contribute to metabolic disorders promoting the progression of insulin-deficient diabetes/T1D development.
31822562	6	44	theme	higher	1039:1044	arg1	abundance					1046:1054	higher abundance	1039:1054	higher abundance of Bacteroidetes	1039:1071	Parameters of lipid metabolism, glucose homeostasis, and mitochondrial respiratory activity were measured in vivo and ex vivo Compared with NOD TLR4+/+ mice, NOD TLR4-/- animals showed lower muscle mass of the small intestine, higher abundance of Bacteroidetes, and lower Firmicutes in the large intestine, along with lower levels of circulating short-chain fatty acids (SCFA).
31822562	4	45	theme	altered	520:526	arg1	functions					539:547	altered intestinal functions	520:547	altered intestinal functions	520:547	We hypothesized that altered intestinal functions contribute to metabolic alterations, which favor accelerated diabetes development in TLR4-deficient (TLR4-/-) NOD mice.
31822562	8	46	dep	lower	1445:1449	arg1	rates					1478:1482	total respiratory exchange rates	1451:1482	total respiratory exchange rates	1451:1482	These mice also exhibited insulin resistance-related abnormalities of energy metabolism, such as lower total respiratory exchange rates and higher hepatic oxidative capacity.
31822562	0	47	theme	microbiota	43:52	arg1	alterations					9:19	Distinct alterations	0:19	Distinct alterations of gut morphology and microbiota	0:52	Distinct alterations of gut morphology and microbiota characterize accelerated diabetes onset in nonobese diabetic mice.
31822562	6	48	theme	homeostasis	852:862	arg1	Parameters					812:821	Parameters	812:821	Parameters of lipid metabolism, glucose homeostasis, and mitochondrial respiratory activity	812:902	Parameters of lipid metabolism, glucose homeostasis, and mitochondrial respiratory activity were measured in vivo and ex vivo Compared with NOD TLR4+/+ mice, NOD TLR4-/- animals showed lower muscle mass of the small intestine, higher abundance of Bacteroidetes, and lower Firmicutes in the large intestine, along with lower levels of circulating short-chain fatty acids (SCFA).
31822562	9	49	theme	metabolic	1653:1661	arg1	disorders					1663:1671	metabolic disorders	1653:1671	metabolic disorders promoting the progression of insulin-deficient diabetes/T1D development	1653:1743	Distinct alterations of gut morphology and microbiota composition associated with reduction of circulating SCFA may contribute to metabolic disorders promoting the progression of insulin-deficient diabetes/T1D development.
31822562	6	50	theme	large	1102:1106	arg1	intestine					1108:1116	the large intestine	1098:1116	the large intestine	1098:1116	Parameters of lipid metabolism, glucose homeostasis, and mitochondrial respiratory activity were measured in vivo and ex vivo Compared with NOD TLR4+/+ mice, NOD TLR4-/- animals showed lower muscle mass of the small intestine, higher abundance of Bacteroidetes, and lower Firmicutes in the large intestine, along with lower levels of circulating short-chain fatty acids (SCFA).
31822562	4	51	theme	TLR4-deficient	634:647	arg1	mice					663:666	TLR4-deficient (TLR4-/-) NOD mice	634:666	TLR4-deficient (TLR4-/-) NOD mice	634:666	We hypothesized that altered intestinal functions contribute to metabolic alterations, which favor accelerated diabetes development in TLR4-deficient (TLR4-/-) NOD mice.
31822562	6	52	from	mass	1010:1013	arg1	intestine					1108:1116	the large intestine	1098:1116	the large intestine	1098:1116	Parameters of lipid metabolism, glucose homeostasis, and mitochondrial respiratory activity were measured in vivo and ex vivo Compared with NOD TLR4+/+ mice, NOD TLR4-/- animals showed lower muscle mass of the small intestine, higher abundance of Bacteroidetes, and lower Firmicutes in the large intestine, along with lower levels of circulating short-chain fatty acids (SCFA).
31822562	2	53	theme	Recent	279:284	arg1	findings					286:293	Recent findings	279:293	Recent findings	279:293	Recent findings point to gut-associated mechanisms in the control of T1D pathogenesis.
31822562	1	54	theme	past	177:180	arg1	decades					182:188	the past decades	173:188	the past decades	173:188	The rising prevalence of type 1 diabetes (T1D) over the past decades has been linked to lifestyle changes, but the underlying mechanisms are largely unknown.
31822562	2	55	theme	T1D	348:350	arg1	pathogenesis					352:363	T1D pathogenesis	348:363	T1D pathogenesis	348:363	Recent findings point to gut-associated mechanisms in the control of T1D pathogenesis.
31822562	8	56	theme	resistance-related	1382:1399	arg1	abnormalities					1401:1413	insulin resistance-related abnormalities	1374:1413	insulin resistance-related abnormalities	1374:1413	These mice also exhibited insulin resistance-related abnormalities of energy metabolism, such as lower total respiratory exchange rates and higher hepatic oxidative capacity.
31822562	8	56	theme	resistance-related	1382:1399	arg1	capacity					1513:1520	lower total respiratory exchange rates and higher hepatic oxidative capacity	1445:1520	lower total respiratory exchange rates and higher hepatic oxidative capacity	1445:1520	These mice also exhibited insulin resistance-related abnormalities of energy metabolism, such as lower total respiratory exchange rates and higher hepatic oxidative capacity.
31822562	7	57	theme	higher	1224:1229	arg1	weight					1236:1241	higher body weight	1224:1241	higher body weight	1224:1241	These changes are associated with higher body weight, hyperlipidemia, and severe insulin and glucose intolerance, all occurring before the onset of diabetes.
31822562	5	58	theme	70-90-day-old	672:684	arg1	TLR4+/+					725:731	70-90-day-old normoglycemic (prediabetic) female NOD TLR4+/+ and NOD TLR4-/- mice	672:752	TLR4+/+	725:731	In 70-90-day-old normoglycemic (prediabetic) female NOD TLR4+/+ and NOD TLR4-/- mice, gut morphology and microbiome composition were analyzed.
31822562	9	59	theme	insulin-deficient	1702:1718	arg1	development					1733:1743	insulin-deficient diabetes/T1D development	1702:1743	insulin-deficient diabetes/T1D development	1702:1743	Distinct alterations of gut morphology and microbiota composition associated with reduction of circulating SCFA may contribute to metabolic disorders promoting the progression of insulin-deficient diabetes/T1D development.
31822562	4	60	theme	accelerated	598:608	arg1	development					619:629	accelerated diabetes development	598:629	accelerated diabetes development	598:629	We hypothesized that altered intestinal functions contribute to metabolic alterations, which favor accelerated diabetes development in TLR4-deficient (TLR4-/-) NOD mice.
31822562	9	61	theme	development	1733:1743	arg1	progression					1687:1697	the progression	1683:1697	the progression of insulin-deficient diabetes/T1D development	1683:1743	Distinct alterations of gut morphology and microbiota composition associated with reduction of circulating SCFA may contribute to metabolic disorders promoting the progression of insulin-deficient diabetes/T1D development.
31822562	8	62	theme	metabolism	1425:1434	arg1	abnormalities					1401:1413	insulin resistance-related abnormalities	1374:1413	insulin resistance-related abnormalities	1374:1413	These mice also exhibited insulin resistance-related abnormalities of energy metabolism, such as lower total respiratory exchange rates and higher hepatic oxidative capacity.
31822562	8	62	theme	metabolism	1425:1434	arg1	capacity					1513:1520	lower total respiratory exchange rates and higher hepatic oxidative capacity	1445:1520	lower total respiratory exchange rates and higher hepatic oxidative capacity	1445:1520	These mice also exhibited insulin resistance-related abnormalities of energy metabolism, such as lower total respiratory exchange rates and higher hepatic oxidative capacity.
31822562	6	63	theme	fatty	1170:1174	arg1	acids					1176:1180	circulating short-chain fatty acids	1146:1180	circulating short-chain fatty acids (SCFA)	1146:1187	Parameters of lipid metabolism, glucose homeostasis, and mitochondrial respiratory activity were measured in vivo and ex vivo Compared with NOD TLR4+/+ mice, NOD TLR4-/- animals showed lower muscle mass of the small intestine, higher abundance of Bacteroidetes, and lower Firmicutes in the large intestine, along with lower levels of circulating short-chain fatty acids (SCFA).
31822562	6	63	theme	fatty	1170:1174	arg1	SCFA					1183:1186	SCFA	1183:1186	SCFA	1183:1186	Parameters of lipid metabolism, glucose homeostasis, and mitochondrial respiratory activity were measured in vivo and ex vivo Compared with NOD TLR4+/+ mice, NOD TLR4-/- animals showed lower muscle mass of the small intestine, higher abundance of Bacteroidetes, and lower Firmicutes in the large intestine, along with lower levels of circulating short-chain fatty acids (SCFA).
31822562	6	64	theme	circulating	1146:1156	arg1	acids					1176:1180	circulating short-chain fatty acids	1146:1180	circulating short-chain fatty acids (SCFA)	1146:1187	Parameters of lipid metabolism, glucose homeostasis, and mitochondrial respiratory activity were measured in vivo and ex vivo Compared with NOD TLR4+/+ mice, NOD TLR4-/- animals showed lower muscle mass of the small intestine, higher abundance of Bacteroidetes, and lower Firmicutes in the large intestine, along with lower levels of circulating short-chain fatty acids (SCFA).
31822562	6	64	theme	circulating	1146:1156	arg1	SCFA					1183:1186	SCFA	1183:1186	SCFA	1183:1186	Parameters of lipid metabolism, glucose homeostasis, and mitochondrial respiratory activity were measured in vivo and ex vivo Compared with NOD TLR4+/+ mice, NOD TLR4-/- animals showed lower muscle mass of the small intestine, higher abundance of Bacteroidetes, and lower Firmicutes in the large intestine, along with lower levels of circulating short-chain fatty acids (SCFA).
31822562	5	65	theme	NOD	721:723	arg1	TLR4+/+					725:731	70-90-day-old normoglycemic (prediabetic) female NOD TLR4+/+ and NOD TLR4-/- mice	672:752	TLR4+/+	725:731	In 70-90-day-old normoglycemic (prediabetic) female NOD TLR4+/+ and NOD TLR4-/- mice, gut morphology and microbiome composition were analyzed.
31822562	1	66	theme	type	146:149	arg1	T1D					163:165	T1D	163:165	T1D	163:165	The rising prevalence of type 1 diabetes (T1D) over the past decades has been linked to lifestyle changes, but the underlying mechanisms are largely unknown.
31822562	1	66	theme	type	146:149	arg1	diabetes					153:160	type 1 diabetes	146:160	type 1 diabetes (T1D)	146:166	The rising prevalence of type 1 diabetes (T1D) over the past decades has been linked to lifestyle changes, but the underlying mechanisms are largely unknown.
31822562	6	67	theme	TLR4-/-	974:980	arg1	animals					982:988	NOD TLR4-/- animals	970:988	NOD TLR4-/- animals	970:988	Parameters of lipid metabolism, glucose homeostasis, and mitochondrial respiratory activity were measured in vivo and ex vivo Compared with NOD TLR4+/+ mice, NOD TLR4-/- animals showed lower muscle mass of the small intestine, higher abundance of Bacteroidetes, and lower Firmicutes in the large intestine, along with lower levels of circulating short-chain fatty acids (SCFA).
31822562	8	68	theme	lower	1445:1449	arg1	capacity					1513:1520	lower total respiratory exchange rates and higher hepatic oxidative capacity	1445:1520	lower total respiratory exchange rates and higher hepatic oxidative capacity	1445:1520	These mice also exhibited insulin resistance-related abnormalities of energy metabolism, such as lower total respiratory exchange rates and higher hepatic oxidative capacity.
31822562	7	69	theme	glucose	1283:1289	arg1	intolerance					1291:1301	glucose intolerance	1283:1301	glucose intolerance	1283:1301	These changes are associated with higher body weight, hyperlipidemia, and severe insulin and glucose intolerance, all occurring before the onset of diabetes.
31822562	5	70	theme	prediabetic	701:711	arg1	TLR4+/+					725:731	70-90-day-old normoglycemic (prediabetic) female NOD TLR4+/+ and NOD TLR4-/- mice	672:752	TLR4+/+	725:731	In 70-90-day-old normoglycemic (prediabetic) female NOD TLR4+/+ and NOD TLR4-/- mice, gut morphology and microbiome composition were analyzed.
31822562	1	71	theme	diabetes	153:160	arg1	prevalence					132:141	The rising prevalence	121:141	The rising prevalence of type 1 diabetes (T1D) over the past decades	121:188	The rising prevalence of type 1 diabetes (T1D) over the past decades has been linked to lifestyle changes, but the underlying mechanisms are largely unknown.
31822562	8	72	theme	respiratory	1457:1467	arg1	rates					1478:1482	total respiratory exchange rates	1451:1482	total respiratory exchange rates	1451:1482	These mice also exhibited insulin resistance-related abnormalities of energy metabolism, such as lower total respiratory exchange rates and higher hepatic oxidative capacity.
31822562	4	73	theme	NOD	659:661	arg1	mice					663:666	TLR4-deficient (TLR4-/-) NOD mice	634:666	TLR4-deficient (TLR4-/-) NOD mice	634:666	We hypothesized that altered intestinal functions contribute to metabolic alterations, which favor accelerated diabetes development in TLR4-deficient (TLR4-/-) NOD mice.
31822562	0	74	theme	diabetic	106:113	arg1	mice					115:118	nonobese diabetic mice	97:118	nonobese diabetic mice	97:118	Distinct alterations of gut morphology and microbiota characterize accelerated diabetes onset in nonobese diabetic mice.
31822562	6	75	theme	TLR4+/+	956:962	arg1	mice					964:967	NOD TLR4+/+ mice	952:967	NOD TLR4+/+ mice	952:967	Parameters of lipid metabolism, glucose homeostasis, and mitochondrial respiratory activity were measured in vivo and ex vivo Compared with NOD TLR4+/+ mice, NOD TLR4-/- animals showed lower muscle mass of the small intestine, higher abundance of Bacteroidetes, and lower Firmicutes in the large intestine, along with lower levels of circulating short-chain fatty acids (SCFA).
31822562	7	76	theme	severe	1264:1269	arg1	insulin					1271:1277	severe insulin	1264:1277	severe insulin	1264:1277	These changes are associated with higher body weight, hyperlipidemia, and severe insulin and glucose intolerance, all occurring before the onset of diabetes.
31822562	8	77	theme	higher	1488:1493	arg1	capacity					1513:1520	lower total respiratory exchange rates and higher hepatic oxidative capacity	1445:1520	lower total respiratory exchange rates and higher hepatic oxidative capacity	1445:1520	These mice also exhibited insulin resistance-related abnormalities of energy metabolism, such as lower total respiratory exchange rates and higher hepatic oxidative capacity.
31822562	9	78	theme	Distinct	1523:1530	arg1	alterations					1532:1542	Distinct alterations	1523:1542	Distinct alterations of gut morphology and microbiota composition associated with reduction of circulating SCFA	1523:1633	Distinct alterations of gut morphology and microbiota composition associated with reduction of circulating SCFA may contribute to metabolic disorders promoting the progression of insulin-deficient diabetes/T1D development.
31822562	6	79	theme	small	1022:1026	arg1	intestine					1028:1036	the small intestine	1018:1036	the small intestine	1018:1036	Parameters of lipid metabolism, glucose homeostasis, and mitochondrial respiratory activity were measured in vivo and ex vivo Compared with NOD TLR4+/+ mice, NOD TLR4-/- animals showed lower muscle mass of the small intestine, higher abundance of Bacteroidetes, and lower Firmicutes in the large intestine, along with lower levels of circulating short-chain fatty acids (SCFA).
31822562	3	80	theme	nonobese	369:376	arg1	NOD					388:390	NOD	388:390	NOD	388:390	In nonobese diabetic (NOD) mice, a model of T1D, diabetes development accelerates after deletion of the Toll-like receptor 4 (TLR4).
31822562	3	80	theme	nonobese	369:376	arg1	diabetic					378:385	nonobese diabetic	369:385	nonobese diabetic (NOD) mice	369:396	In nonobese diabetic (NOD) mice, a model of T1D, diabetes development accelerates after deletion of the Toll-like receptor 4 (TLR4).
31822562	8	81	theme	oxidative	1503:1511	arg1	capacity					1513:1520	lower total respiratory exchange rates and higher hepatic oxidative capacity	1445:1520	lower total respiratory exchange rates and higher hepatic oxidative capacity	1445:1520	These mice also exhibited insulin resistance-related abnormalities of energy metabolism, such as lower total respiratory exchange rates and higher hepatic oxidative capacity.
31822562	2	82	theme	pathogenesis	352:363	arg1	control					337:343	the control	333:343	the control of T1D pathogenesis	333:363	Recent findings point to gut-associated mechanisms in the control of T1D pathogenesis.
31822562	0	83	theme	gut	24:26	arg1	morphology					28:37	gut morphology	24:37	gut morphology	24:37	Distinct alterations of gut morphology and microbiota characterize accelerated diabetes onset in nonobese diabetic mice.
31822562	3	84	theme	diabetes	415:422	arg1	development					424:434	diabetes development	415:434	diabetes development	415:434	In nonobese diabetic (NOD) mice, a model of T1D, diabetes development accelerates after deletion of the Toll-like receptor 4 (TLR4).
31822562	5	85	theme	TLR4-/-	741:747	arg1	mice					749:752	70-90-day-old normoglycemic (prediabetic) female NOD TLR4+/+ and NOD TLR4-/- mice	672:752	mice	749:752	In 70-90-day-old normoglycemic (prediabetic) female NOD TLR4+/+ and NOD TLR4-/- mice, gut morphology and microbiome composition were analyzed.
31822562	6	86	theme	metabolism	832:841	arg1	Parameters					812:821	Parameters	812:821	Parameters of lipid metabolism, glucose homeostasis, and mitochondrial respiratory activity	812:902	Parameters of lipid metabolism, glucose homeostasis, and mitochondrial respiratory activity were measured in vivo and ex vivo Compared with NOD TLR4+/+ mice, NOD TLR4-/- animals showed lower muscle mass of the small intestine, higher abundance of Bacteroidetes, and lower Firmicutes in the large intestine, along with lower levels of circulating short-chain fatty acids (SCFA).
31822562	9	87	theme	morphology	1551:1560	arg1	alterations					1532:1542	Distinct alterations	1523:1542	Distinct alterations of gut morphology and microbiota composition associated with reduction of circulating SCFA	1523:1633	Distinct alterations of gut morphology and microbiota composition associated with reduction of circulating SCFA may contribute to metabolic disorders promoting the progression of insulin-deficient diabetes/T1D development.
31822562	7	88	theme	body	1231:1234	arg1	weight					1236:1241	higher body weight	1224:1241	higher body weight	1224:1241	These changes are associated with higher body weight, hyperlipidemia, and severe insulin and glucose intolerance, all occurring before the onset of diabetes.
31822562	3	89	theme	T1D	410:412	arg1	mice					393:396	nonobese diabetic (NOD) mice	369:396	nonobese diabetic (NOD) mice	369:396	In nonobese diabetic (NOD) mice, a model of T1D, diabetes development accelerates after deletion of the Toll-like receptor 4 (TLR4).
31822562	3	89	theme	T1D	410:412	arg1	model					401:405	a model	399:405	a model of T1D	399:412	In nonobese diabetic (NOD) mice, a model of T1D, diabetes development accelerates after deletion of the Toll-like receptor 4 (TLR4).
31822562	9	90	theme	microbiota	1566:1575	arg1	composition					1577:1587	microbiota composition	1566:1587	microbiota composition	1566:1587	Distinct alterations of gut morphology and microbiota composition associated with reduction of circulating SCFA may contribute to metabolic disorders promoting the progression of insulin-deficient diabetes/T1D development.
31822562	6	91	from	Firmicutes	1084:1093	arg1	intestine					1108:1116	the large intestine	1098:1116	the large intestine	1098:1116	Parameters of lipid metabolism, glucose homeostasis, and mitochondrial respiratory activity were measured in vivo and ex vivo Compared with NOD TLR4+/+ mice, NOD TLR4-/- animals showed lower muscle mass of the small intestine, higher abundance of Bacteroidetes, and lower Firmicutes in the large intestine, along with lower levels of circulating short-chain fatty acids (SCFA).
31822562	1	92	attach	linked	199:204	arg1	changes					219:225	lifestyle changes	209:225	lifestyle changes	209:225	The rising prevalence of type 1 diabetes (T1D) over the past decades has been linked to lifestyle changes, but the underlying mechanisms are largely unknown.
31822562	1	92	attach	linked	199:204	arg2	prevalence					132:141	The rising prevalence	121:141	The rising prevalence of type 1 diabetes (T1D) over the past decades	121:188	The rising prevalence of type 1 diabetes (T1D) over the past decades has been linked to lifestyle changes, but the underlying mechanisms are largely unknown.
31822562	4	93	theme	metabolic	563:571	arg1	alterations					573:583	metabolic alterations	563:583	metabolic alterations	563:583	We hypothesized that altered intestinal functions contribute to metabolic alterations, which favor accelerated diabetes development in TLR4-deficient (TLR4-/-) NOD mice.
31822562	6	94	from	abundance	1046:1054	arg1	intestine					1108:1116	the large intestine	1098:1116	the large intestine	1098:1116	Parameters of lipid metabolism, glucose homeostasis, and mitochondrial respiratory activity were measured in vivo and ex vivo Compared with NOD TLR4+/+ mice, NOD TLR4-/- animals showed lower muscle mass of the small intestine, higher abundance of Bacteroidetes, and lower Firmicutes in the large intestine, along with lower levels of circulating short-chain fatty acids (SCFA).
31822562	6	95	theme	Bacteroidetes	1059:1071	arg1	mass					1010:1013	lower muscle mass	997:1013	lower muscle mass of the small intestine	997:1036	Parameters of lipid metabolism, glucose homeostasis, and mitochondrial respiratory activity were measured in vivo and ex vivo Compared with NOD TLR4+/+ mice, NOD TLR4-/- animals showed lower muscle mass of the small intestine, higher abundance of Bacteroidetes, and lower Firmicutes in the large intestine, along with lower levels of circulating short-chain fatty acids (SCFA).
31822562	6	95	theme	Bacteroidetes	1059:1071	arg1	abundance					1046:1054	higher abundance	1039:1054	higher abundance of Bacteroidetes	1039:1071	Parameters of lipid metabolism, glucose homeostasis, and mitochondrial respiratory activity were measured in vivo and ex vivo Compared with NOD TLR4+/+ mice, NOD TLR4-/- animals showed lower muscle mass of the small intestine, higher abundance of Bacteroidetes, and lower Firmicutes in the large intestine, along with lower levels of circulating short-chain fatty acids (SCFA).
31822562	6	95	theme	Bacteroidetes	1059:1071	arg1	Firmicutes					1084:1093	lower Firmicutes	1078:1093	lower Firmicutes in the large intestine	1078:1116	Parameters of lipid metabolism, glucose homeostasis, and mitochondrial respiratory activity were measured in vivo and ex vivo Compared with NOD TLR4+/+ mice, NOD TLR4-/- animals showed lower muscle mass of the small intestine, higher abundance of Bacteroidetes, and lower Firmicutes in the large intestine, along with lower levels of circulating short-chain fatty acids (SCFA).
31822562	3	96	theme	receptor	480:487	arg1	deletion					454:461	deletion	454:461	deletion of the Toll-like receptor 4 (TLR4)	454:496	In nonobese diabetic (NOD) mice, a model of T1D, diabetes development accelerates after deletion of the Toll-like receptor 4 (TLR4).
29710519	9	0	theme	Serum	1136:1140	arg1	analysis					1154:1161	Serum biochemical analysis	1136:1161	Serum biochemical analysis	1136:1161	Serum biochemical analysis indicated that SLBZP decreased the serum level of total cholesterol (TC) and improved liver function.
29710519	6	1	theme	expression	862:871	arg1	profiles					873:880	The expression profiles	858:880	The expression profiles of liver TLR4 pathway related protein	858:918	The expression profiles of liver TLR4 pathway related protein was confirmed by western blotting.
29710519	15	2	from	abundance	2044:2052	arg1	vein					2125:2128	the portal vein	2114:2128	the portal vein	2114:2128	CONCLUSION Taken together, these results suggest that the effects of SLBZP against NAFLD may be related to the increased abundance of beneficial gut microbiota and decreased levels of LPS in the portal vein.
29710519	4	3	theme	NAFLD	576:580	arg1	model					582:586	an NAFLD model	573:586	an NAFLD model in rats given a high-fat diet (HFD), administered different interventions	573:660	METHODS We established an NAFLD model in rats given a high-fat diet (HFD), administered different interventions and measured serum biochemical indices and inflammatory factors.
29710519	13	4	theme	intestinal	1742:1751	arg1	composition					1764:1774	intestinal microbiota composition	1742:1774	intestinal microbiota composition	1742:1774	In taxonomy-based analysis, compared with control rats, SLBZP-treated rats showed obvious changes in intestinal microbiota composition.
29710519	3	5	theme	action	531:536	arg1	mechanism					518:526	its mechanism	514:526	its mechanism of action	514:536	SLBZP has improved NAFLD symptoms; however, its mechanism of action is unknown.
29710519	1	6	theme	other	255:259	arg1	diseases					271:278	other metabolic diseases	255:278	other metabolic diseases	255:278	BACKGROUND Worldwide, non-alcoholic fatty liver disease (NAFLD) is a common chronic liver disease closely associated with obesity, diabetes and other metabolic diseases.
29710519	14	7	theme	acid	1847:1850	arg1	Anaerostipes					1909:1920	Anaerostipes	1909:1920	Anaerostipes	1909:1920	Moreover, SLBZP increased the relative abundance of short-chain fatty acid (SCFA)-producing bacteria, including Bifidobacterium and Anaerostipes.
29710519	14	7	theme	acid	1847:1850	arg1	bacteria					1869:1876	short-chain fatty acid (SCFA)-producing bacteria	1829:1876	short-chain fatty acid (SCFA)-producing bacteria	1829:1876	Moreover, SLBZP increased the relative abundance of short-chain fatty acid (SCFA)-producing bacteria, including Bifidobacterium and Anaerostipes.
29710519	14	7	theme	acid	1847:1850	arg1	Bifidobacterium					1889:1903	Bifidobacterium	1889:1903	Bifidobacterium	1889:1903	Moreover, SLBZP increased the relative abundance of short-chain fatty acid (SCFA)-producing bacteria, including Bifidobacterium and Anaerostipes.
29710519	4	8	theme	administered	625:636	arg1	diet					613:616	a high-fat diet	602:616	a high-fat diet (HFD)	602:622	METHODS We established an NAFLD model in rats given a high-fat diet (HFD), administered different interventions and measured serum biochemical indices and inflammatory factors.
29710519	4	8	theme	administered	625:636	arg1	interventions					648:660	different interventions	638:660	administered different interventions	625:660	METHODS We established an NAFLD model in rats given a high-fat diet (HFD), administered different interventions and measured serum biochemical indices and inflammatory factors.
29710519	2	9	contain	has	369:371	arg1	powder					297:302	Shenling Baizhu powder	281:302	Shenling Baizhu powder (SLBZP)	281:310	Shenling Baizhu powder (SLBZP), a formulation of a variety of natural medicinal plants, has hepatoprotective properties and clinical efficacy in treating non-infectious intestinal disease.
29710519	2	9	contain	has	369:371	arg2	properties					390:399	hepatoprotective properties	373:399	hepatoprotective properties	373:399	Shenling Baizhu powder (SLBZP), a formulation of a variety of natural medicinal plants, has hepatoprotective properties and clinical efficacy in treating non-infectious intestinal disease.
29710519	2	9	contain	has	369:371	arg1	formulation					315:325	a formulation	313:325	a formulation of a variety of natural medicinal plants	313:366	Shenling Baizhu powder (SLBZP), a formulation of a variety of natural medicinal plants, has hepatoprotective properties and clinical efficacy in treating non-infectious intestinal disease.
29710519	2	9	contain	has	369:371	arg2	efficacy					414:421	clinical efficacy	405:421	clinical efficacy	405:421	Shenling Baizhu powder (SLBZP), a formulation of a variety of natural medicinal plants, has hepatoprotective properties and clinical efficacy in treating non-infectious intestinal disease.
29710519	2	9	contain	has	369:371	arg1	SLBZP					305:309	SLBZP	305:309	SLBZP	305:309	Shenling Baizhu powder (SLBZP), a formulation of a variety of natural medicinal plants, has hepatoprotective properties and clinical efficacy in treating non-infectious intestinal disease.
29710519	14	10	theme	bacteria	1869:1876	arg1	abundance					1816:1824	the relative abundance	1803:1824	the relative abundance of short-chain fatty acid (SCFA)-producing bacteria, including Bifidobacterium and Anaerostipes	1803:1920	Moreover, SLBZP increased the relative abundance of short-chain fatty acid (SCFA)-producing bacteria, including Bifidobacterium and Anaerostipes.
29710519	4	11	theme	high-fat	604:611	arg1	diet					613:616	a high-fat diet	602:616	a high-fat diet (HFD)	602:622	METHODS We established an NAFLD model in rats given a high-fat diet (HFD), administered different interventions and measured serum biochemical indices and inflammatory factors.
29710519	4	11	theme	high-fat	604:611	arg1	interventions					648:660	different interventions	638:660	administered different interventions	625:660	METHODS We established an NAFLD model in rats given a high-fat diet (HFD), administered different interventions and measured serum biochemical indices and inflammatory factors.
29710519	4	11	theme	high-fat	604:611	arg1	HFD					619:621	HFD	619:621	HFD	619:621	METHODS We established an NAFLD model in rats given a high-fat diet (HFD), administered different interventions and measured serum biochemical indices and inflammatory factors.
29710519	9	12	theme	serum	1198:1202	arg1	level					1204:1208	the serum level	1194:1208	the serum level of total cholesterol (TC)	1194:1234	Serum biochemical analysis indicated that SLBZP decreased the serum level of total cholesterol (TC) and improved liver function.
29710519	2	13	theme	Baizhu	290:295	arg1	powder					297:302	Shenling Baizhu powder	281:302	Shenling Baizhu powder (SLBZP)	281:310	Shenling Baizhu powder (SLBZP), a formulation of a variety of natural medicinal plants, has hepatoprotective properties and clinical efficacy in treating non-infectious intestinal disease.
29710519	2	13	theme	Baizhu	290:295	arg1	formulation					315:325	a formulation	313:325	a formulation of a variety of natural medicinal plants	313:366	Shenling Baizhu powder (SLBZP), a formulation of a variety of natural medicinal plants, has hepatoprotective properties and clinical efficacy in treating non-infectious intestinal disease.
29710519	2	13	theme	Baizhu	290:295	arg1	SLBZP					305:309	SLBZP	305:309	SLBZP	305:309	Shenling Baizhu powder (SLBZP), a formulation of a variety of natural medicinal plants, has hepatoprotective properties and clinical efficacy in treating non-infectious intestinal disease.
29710519	1	14	theme	chronic	187:193	arg1	Worldwide					122:130	BACKGROUND Worldwide	111:130	BACKGROUND Worldwide	111:130	BACKGROUND Worldwide, non-alcoholic fatty liver disease (NAFLD) is a common chronic liver disease closely associated with obesity, diabetes and other metabolic diseases.
29710519	1	14	theme	chronic	187:193	arg1	disease					201:207	a common chronic liver disease	178:207	a common chronic liver disease closely associated with obesity, diabetes and other metabolic diseases	178:278	BACKGROUND Worldwide, non-alcoholic fatty liver disease (NAFLD) is a common chronic liver disease closely associated with obesity, diabetes and other metabolic diseases.
29710519	12	15	theme	Microbiome	1552:1561	arg1	analysis					1563:1570	Microbiome analysis	1552:1570	Microbiome analysis	1552:1570	Microbiome analysis revealed that SLBZP improved the abundance of intestinal microbiota.
29710519	7	16	theme	sequencing	1031:1040	arg1	technique					1042:1050	a 16S rDNA sequencing technique	1020:1050	a 16S rDNA sequencing technique	1020:1050	Changes in intestinal microbiota composition were analyzed using a 16S rDNA sequencing technique.
29710519	6	17	theme	related	904:910	arg1	protein					912:918	liver TLR4 pathway related protein	885:918	liver TLR4 pathway related protein	885:918	The expression profiles of liver TLR4 pathway related protein was confirmed by western blotting.
29710519	4	18	theme	biochemical	681:691	arg1	indices					693:699	serum biochemical indices	675:699	serum biochemical indices	675:699	METHODS We established an NAFLD model in rats given a high-fat diet (HFD), administered different interventions and measured serum biochemical indices and inflammatory factors.
29710519	13	19	theme	SLBZP-treated	1697:1709	arg1	rats					1711:1714	SLBZP-treated rats	1697:1714	SLBZP-treated rats	1697:1714	In taxonomy-based analysis, compared with control rats, SLBZP-treated rats showed obvious changes in intestinal microbiota composition.
29710519	7	20	theme	16S	1022:1024	arg1	technique					1042:1050	a 16S rDNA sequencing technique	1020:1050	a 16S rDNA sequencing technique	1020:1050	Changes in intestinal microbiota composition were analyzed using a 16S rDNA sequencing technique.
29710519	9	21	theme	cholesterol	1219:1229	arg1	level					1204:1208	the serum level	1194:1208	the serum level of total cholesterol (TC)	1194:1234	Serum biochemical analysis indicated that SLBZP decreased the serum level of total cholesterol (TC) and improved liver function.
29710519	7	22	theme	intestinal	966:975	arg1	composition					988:998	intestinal microbiota composition	966:998	intestinal microbiota composition	966:998	Changes in intestinal microbiota composition were analyzed using a 16S rDNA sequencing technique.
29710519	14	23	theme	fatty	1841:1845	arg1	SCFA					1853:1856	SCFA	1853:1856	SCFA	1853:1856	Moreover, SLBZP increased the relative abundance of short-chain fatty acid (SCFA)-producing bacteria, including Bifidobacterium and Anaerostipes.
29710519	14	23	theme	fatty	1841:1845	arg1	acid					1847:1850	short-chain fatty acid	1829:1850	short-chain fatty acid (SCFA)-producing bacteria	1829:1876	Moreover, SLBZP increased the relative abundance of short-chain fatty acid (SCFA)-producing bacteria, including Bifidobacterium and Anaerostipes.
29710519	6	24	theme	TLR4	891:894	arg1	protein					912:918	liver TLR4 pathway related protein	885:918	liver TLR4 pathway related protein	885:918	The expression profiles of liver TLR4 pathway related protein was confirmed by western blotting.
29710519	1	25	theme	non-alcoholic	133:145	arg1	NAFLD					168:172	NAFLD	168:172	NAFLD	168:172	BACKGROUND Worldwide, non-alcoholic fatty liver disease (NAFLD) is a common chronic liver disease closely associated with obesity, diabetes and other metabolic diseases.
29710519	1	25	theme	non-alcoholic	133:145	arg1	disease					159:165	non-alcoholic fatty liver disease	133:165	non-alcoholic fatty liver disease (NAFLD)	133:173	BACKGROUND Worldwide, non-alcoholic fatty liver disease (NAFLD) is a common chronic liver disease closely associated with obesity, diabetes and other metabolic diseases.
29710519	1	25	theme	non-alcoholic	133:145	arg1	Worldwide					122:130	BACKGROUND Worldwide	111:130	BACKGROUND Worldwide	111:130	BACKGROUND Worldwide, non-alcoholic fatty liver disease (NAFLD) is a common chronic liver disease closely associated with obesity, diabetes and other metabolic diseases.
29710519	0	26	theme	intestinal	52:61	arg1	microbiota					63:72	intestinal microbiota	52:72	intestinal microbiota in high-fat diet-induced NAFLD rats	52:108	Effects of shenling baizhu powder herbal formula on intestinal microbiota in high-fat diet-induced NAFLD rats.
29710519	5	27	theme	oil	790:792	arg1	O					798:798	oil red O	790:798	oil red O	790:798	Liver tissues were stained with hematoxylin and eosin (HE) and oil red O, and colon tissues were analyzed by immunohistochemistry.
29710519	1	28	theme	liver	153:157	arg1	NAFLD					168:172	NAFLD	168:172	NAFLD	168:172	BACKGROUND Worldwide, non-alcoholic fatty liver disease (NAFLD) is a common chronic liver disease closely associated with obesity, diabetes and other metabolic diseases.
29710519	1	28	theme	liver	153:157	arg1	disease					159:165	non-alcoholic fatty liver disease	133:165	non-alcoholic fatty liver disease (NAFLD)	133:173	BACKGROUND Worldwide, non-alcoholic fatty liver disease (NAFLD) is a common chronic liver disease closely associated with obesity, diabetes and other metabolic diseases.
29710519	1	28	theme	liver	153:157	arg1	Worldwide					122:130	BACKGROUND Worldwide	111:130	BACKGROUND Worldwide	111:130	BACKGROUND Worldwide, non-alcoholic fatty liver disease (NAFLD) is a common chronic liver disease closely associated with obesity, diabetes and other metabolic diseases.
29710519	4	29	from	model	582:586	arg1	rats					591:594	rats	591:594	rats given a high-fat diet (HFD), administered different interventions	591:660	METHODS We established an NAFLD model in rats given a high-fat diet (HFD), administered different interventions and measured serum biochemical indices and inflammatory factors.
29710519	9	30	theme	liver	1249:1253	arg1	function					1255:1262	liver function	1249:1262	liver function	1249:1262	Serum biochemical analysis indicated that SLBZP decreased the serum level of total cholesterol (TC) and improved liver function.
29710519	0	31	from	Effects	0:6	arg1	microbiota					63:72	intestinal microbiota	52:72	intestinal microbiota in high-fat diet-induced NAFLD rats	52:108	Effects of shenling baizhu powder herbal formula on intestinal microbiota in high-fat diet-induced NAFLD rats.
29710519	0	32	theme	diet-induced	86:97	arg1	rats					105:108	high-fat diet-induced NAFLD rats	77:108	high-fat diet-induced NAFLD rats	77:108	Effects of shenling baizhu powder herbal formula on intestinal microbiota in high-fat diet-induced NAFLD rats.
29710519	4	33	dep	METHODS	550:556	arg1	measured					666:673	measured	666:673	measured serum biochemical indices and inflammatory factors	666:724	METHODS We established an NAFLD model in rats given a high-fat diet (HFD), administered different interventions and measured serum biochemical indices and inflammatory factors.
29710519	4	33	dep	METHODS	550:556	arg1	established					561:571	established	561:571	established an NAFLD model in rats given a high-fat diet (HFD), administered different interventions	561:660	METHODS We established an NAFLD model in rats given a high-fat diet (HFD), administered different interventions and measured serum biochemical indices and inflammatory factors.
29710519	5	34	theme	Liver	727:731	arg1	tissues					733:739	Liver tissues	727:739	Liver tissues	727:739	Liver tissues were stained with hematoxylin and eosin (HE) and oil red O, and colon tissues were analyzed by immunohistochemistry.
29710519	12	35	theme	intestinal	1618:1627	arg1	microbiota					1629:1638	intestinal microbiota	1618:1638	intestinal microbiota	1618:1638	Microbiome analysis revealed that SLBZP improved the abundance of intestinal microbiota.
29710519	2	36	theme	medicinal	351:359	arg1	plants					361:366	natural medicinal plants	343:366	natural medicinal plants	343:366	Shenling Baizhu powder (SLBZP), a formulation of a variety of natural medicinal plants, has hepatoprotective properties and clinical efficacy in treating non-infectious intestinal disease.
29710519	1	37	theme	metabolic	261:269	arg1	diseases					271:278	other metabolic diseases	255:278	other metabolic diseases	255:278	BACKGROUND Worldwide, non-alcoholic fatty liver disease (NAFLD) is a common chronic liver disease closely associated with obesity, diabetes and other metabolic diseases.
29710519	15	38	theme	SLBZP	1992:1996	arg1	related					2019:2025	related	2019:2025	related	2019:2025	CONCLUSION Taken together, these results suggest that the effects of SLBZP against NAFLD may be related to the increased abundance of beneficial gut microbiota and decreased levels of LPS in the portal vein.
29710519	15	38	theme	SLBZP	1992:1996	arg1	effects					1981:1987	the effects	1977:1987	the effects of SLBZP against NAFLD	1977:2010	CONCLUSION Taken together, these results suggest that the effects of SLBZP against NAFLD may be related to the increased abundance of beneficial gut microbiota and decreased levels of LPS in the portal vein.
29710519	11	39	theme	Pathological	1454:1465	arg1	examination					1467:1477	Pathological examination	1454:1477	Pathological examination	1454:1477	Pathological examination showed that SLBZP alleviates hepatic steatosis and repairs colon mucosa.
29710519	0	40	theme	shenling	11:18	arg1	formula					41:47	shenling baizhu powder herbal formula	11:47	shenling baizhu powder herbal formula	11:47	Effects of shenling baizhu powder herbal formula on intestinal microbiota in high-fat diet-induced NAFLD rats.
29710519	2	41	theme	hepatoprotective	373:388	arg1	properties					390:399	hepatoprotective properties	373:399	hepatoprotective properties	373:399	Shenling Baizhu powder (SLBZP), a formulation of a variety of natural medicinal plants, has hepatoprotective properties and clinical efficacy in treating non-infectious intestinal disease.
29710519	0	42	theme	powder	27:32	arg1	formula					41:47	shenling baizhu powder herbal formula	11:47	shenling baizhu powder herbal formula	11:47	Effects of shenling baizhu powder herbal formula on intestinal microbiota in high-fat diet-induced NAFLD rats.
29710519	8	43	theme	note	1064:1067	arg1	RESULTS					1053:1059	RESULTS	1053:1059	RESULTS Of note, SLBZP	1053:1074	RESULTS Of note, SLBZP effectively reduced body weight in HFD-fed rats (p < 0.05).
29710519	14	44	theme	-producing	1858:1867	arg1	Anaerostipes					1909:1920	Anaerostipes	1909:1920	Anaerostipes	1909:1920	Moreover, SLBZP increased the relative abundance of short-chain fatty acid (SCFA)-producing bacteria, including Bifidobacterium and Anaerostipes.
29710519	14	44	theme	-producing	1858:1867	arg1	bacteria					1869:1876	short-chain fatty acid (SCFA)-producing bacteria	1829:1876	short-chain fatty acid (SCFA)-producing bacteria	1829:1876	Moreover, SLBZP increased the relative abundance of short-chain fatty acid (SCFA)-producing bacteria, including Bifidobacterium and Anaerostipes.
29710519	14	44	theme	-producing	1858:1867	arg1	Bifidobacterium					1889:1903	Bifidobacterium	1889:1903	Bifidobacterium	1889:1903	Moreover, SLBZP increased the relative abundance of short-chain fatty acid (SCFA)-producing bacteria, including Bifidobacterium and Anaerostipes.
29710519	2	45	theme	intestinal	450:459	arg1	disease					461:467	non-infectious intestinal disease	435:467	non-infectious intestinal disease	435:467	Shenling Baizhu powder (SLBZP), a formulation of a variety of natural medicinal plants, has hepatoprotective properties and clinical efficacy in treating non-infectious intestinal disease.
29710519	10	46	theme	protein	1445:1451	arg1	expression					1410:1419	the expression	1406:1419	the expression of TLR4 pathway related protein	1406:1451	Additionally, SLBZP decreased the serum level of endotoxin, tumor necrosis factor α (TNF-α), interleukin-1β (IL-β) (p < 0.05), and decreased the expression of TLR4 pathway related protein.
29710519	10	47	theme	serum	1299:1303	arg1	level					1305:1309	the serum level	1295:1309	the serum level of endotoxin, tumor necrosis factor α (TNF-α), interleukin-1β (IL-β) (p < 0.05),	1295:1390	Additionally, SLBZP decreased the serum level of endotoxin, tumor necrosis factor α (TNF-α), interleukin-1β (IL-β) (p < 0.05), and decreased the expression of TLR4 pathway related protein.
29710519	3	48	theme	improved	480:487	arg1	symptoms					495:502	improved NAFLD symptoms	480:502	improved NAFLD symptoms	480:502	SLBZP has improved NAFLD symptoms; however, its mechanism of action is unknown.
29710519	0	49	theme	formula	41:47	arg1	Effects					0:6	Effects	0:6	Effects of shenling baizhu powder herbal formula on intestinal microbiota in high-fat diet-induced NAFLD rats.	0:109	Effects of shenling baizhu powder herbal formula on intestinal microbiota in high-fat diet-induced NAFLD rats.
29710519	15	50	theme	decreased	2087:2095	arg1	levels					2097:2102	decreased levels	2087:2102	decreased levels of LPS	2087:2109	CONCLUSION Taken together, these results suggest that the effects of SLBZP against NAFLD may be related to the increased abundance of beneficial gut microbiota and decreased levels of LPS in the portal vein.
29710519	10	51	theme	pathway	1429:1435	arg1	protein					1445:1451	TLR4 pathway related protein	1424:1451	TLR4 pathway related protein	1424:1451	Additionally, SLBZP decreased the serum level of endotoxin, tumor necrosis factor α (TNF-α), interleukin-1β (IL-β) (p < 0.05), and decreased the expression of TLR4 pathway related protein.
29710519	15	52	theme	microbiota	2072:2081	arg1	abundance					2044:2052	the increased abundance	2030:2052	the increased abundance of beneficial gut microbiota and decreased levels of LPS in the portal vein	2030:2128	CONCLUSION Taken together, these results suggest that the effects of SLBZP against NAFLD may be related to the increased abundance of beneficial gut microbiota and decreased levels of LPS in the portal vein.
29710519	14	53	theme	relative	1807:1814	arg1	abundance					1816:1824	the relative abundance	1803:1824	the relative abundance of short-chain fatty acid (SCFA)-producing bacteria, including Bifidobacterium and Anaerostipes	1803:1920	Moreover, SLBZP increased the relative abundance of short-chain fatty acid (SCFA)-producing bacteria, including Bifidobacterium and Anaerostipes.
29710519	6	54	theme	liver	885:889	arg1	protein					912:918	liver TLR4 pathway related protein	885:918	liver TLR4 pathway related protein	885:918	The expression profiles of liver TLR4 pathway related protein was confirmed by western blotting.
29710519	15	55	theme	beneficial	2057:2066	arg1	microbiota					2072:2081	beneficial gut microbiota	2057:2081	beneficial gut microbiota	2057:2081	CONCLUSION Taken together, these results suggest that the effects of SLBZP against NAFLD may be related to the increased abundance of beneficial gut microbiota and decreased levels of LPS in the portal vein.
29710519	8	56	theme	HFD-fed	1111:1117	arg1	p < 0.05					1125:1132	p < 0.05	1125:1132	p < 0.05	1125:1132	RESULTS Of note, SLBZP effectively reduced body weight in HFD-fed rats (p < 0.05).
29710519	8	56	theme	HFD-fed	1111:1117	arg1	rats					1119:1122	HFD-fed rats	1111:1122	HFD-fed rats (p < 0.05)	1111:1133	RESULTS Of note, SLBZP effectively reduced body weight in HFD-fed rats (p < 0.05).
29710519	9	57	theme	biochemical	1142:1152	arg1	analysis					1154:1161	Serum biochemical analysis	1136:1161	Serum biochemical analysis	1136:1161	Serum biochemical analysis indicated that SLBZP decreased the serum level of total cholesterol (TC) and improved liver function.
29710519	2	58	theme	Shenling	281:288	arg1	powder					297:302	Shenling Baizhu powder	281:302	Shenling Baizhu powder (SLBZP)	281:310	Shenling Baizhu powder (SLBZP), a formulation of a variety of natural medicinal plants, has hepatoprotective properties and clinical efficacy in treating non-infectious intestinal disease.
29710519	2	58	theme	Shenling	281:288	arg1	formulation					315:325	a formulation	313:325	a formulation of a variety of natural medicinal plants	313:366	Shenling Baizhu powder (SLBZP), a formulation of a variety of natural medicinal plants, has hepatoprotective properties and clinical efficacy in treating non-infectious intestinal disease.
29710519	2	58	theme	Shenling	281:288	arg1	SLBZP					305:309	SLBZP	305:309	SLBZP	305:309	Shenling Baizhu powder (SLBZP), a formulation of a variety of natural medicinal plants, has hepatoprotective properties and clinical efficacy in treating non-infectious intestinal disease.
29710519	11	59	theme	colon	1538:1542	arg1	mucosa					1544:1549	colon mucosa	1538:1549	colon mucosa	1538:1549	Pathological examination showed that SLBZP alleviates hepatic steatosis and repairs colon mucosa.
29710519	13	60	theme	microbiota	1753:1762	arg1	composition					1764:1774	intestinal microbiota composition	1742:1774	intestinal microbiota composition	1742:1774	In taxonomy-based analysis, compared with control rats, SLBZP-treated rats showed obvious changes in intestinal microbiota composition.
29710519	10	61	theme	necrosis	1331:1338	arg1	endotoxin					1314:1322	endotoxin	1314:1322	endotoxin	1314:1322	Additionally, SLBZP decreased the serum level of endotoxin, tumor necrosis factor α (TNF-α), interleukin-1β (IL-β) (p < 0.05), and decreased the expression of TLR4 pathway related protein.
29710519	10	61	theme	necrosis	1331:1338	arg1	factor					1340:1345	tumor necrosis factor α	1325:1347	tumor necrosis factor α (TNF-α)	1325:1355	Additionally, SLBZP decreased the serum level of endotoxin, tumor necrosis factor α (TNF-α), interleukin-1β (IL-β) (p < 0.05), and decreased the expression of TLR4 pathway related protein.
29710519	10	61	theme	necrosis	1331:1338	arg1	TNF-α					1350:1354	TNF-α	1350:1354	TNF-α	1350:1354	Additionally, SLBZP decreased the serum level of endotoxin, tumor necrosis factor α (TNF-α), interleukin-1β (IL-β) (p < 0.05), and decreased the expression of TLR4 pathway related protein.
29710519	7	62	from	Changes	955:961	arg1	composition					988:998	intestinal microbiota composition	966:998	intestinal microbiota composition	966:998	Changes in intestinal microbiota composition were analyzed using a 16S rDNA sequencing technique.
29710519	5	63	theme	colon	805:809	arg1	tissues					811:817	colon tissues	805:817	colon tissues	805:817	Liver tissues were stained with hematoxylin and eosin (HE) and oil red O, and colon tissues were analyzed by immunohistochemistry.
29710519	11	64	theme	hepatic	1508:1514	arg1	steatosis					1516:1524	hepatic steatosis	1508:1524	hepatic steatosis	1508:1524	Pathological examination showed that SLBZP alleviates hepatic steatosis and repairs colon mucosa.
29710519	6	65	theme	western	937:943	arg1	blotting					945:952	western blotting	937:952	western blotting	937:952	The expression profiles of liver TLR4 pathway related protein was confirmed by western blotting.
29710519	13	66	theme	obvious	1723:1729	arg1	changes					1731:1737	obvious changes	1723:1737	obvious changes in intestinal microbiota composition	1723:1774	In taxonomy-based analysis, compared with control rats, SLBZP-treated rats showed obvious changes in intestinal microbiota composition.
29710519	5	67	theme	red	794:796	arg1	O					798:798	oil red O	790:798	oil red O	790:798	Liver tissues were stained with hematoxylin and eosin (HE) and oil red O, and colon tissues were analyzed by immunohistochemistry.
29710519	13	68	theme	control	1683:1689	arg1	rats					1691:1694	control rats	1683:1694	control rats	1683:1694	In taxonomy-based analysis, compared with control rats, SLBZP-treated rats showed obvious changes in intestinal microbiota composition.
29710519	7	69	theme	rDNA	1026:1029	arg1	technique					1042:1050	a 16S rDNA sequencing technique	1020:1050	a 16S rDNA sequencing technique	1020:1050	Changes in intestinal microbiota composition were analyzed using a 16S rDNA sequencing technique.
29710519	1	70	theme	liver	195:199	arg1	Worldwide					122:130	BACKGROUND Worldwide	111:130	BACKGROUND Worldwide	111:130	BACKGROUND Worldwide, non-alcoholic fatty liver disease (NAFLD) is a common chronic liver disease closely associated with obesity, diabetes and other metabolic diseases.
29710519	1	70	theme	liver	195:199	arg1	disease					201:207	a common chronic liver disease	178:207	a common chronic liver disease closely associated with obesity, diabetes and other metabolic diseases	178:278	BACKGROUND Worldwide, non-alcoholic fatty liver disease (NAFLD) is a common chronic liver disease closely associated with obesity, diabetes and other metabolic diseases.
29710519	6	71	theme	protein	912:918	arg1	profiles					873:880	The expression profiles	858:880	The expression profiles of liver TLR4 pathway related protein	858:918	The expression profiles of liver TLR4 pathway related protein was confirmed by western blotting.
29710519	0	72	from	microbiota	63:72	arg1	rats					105:108	high-fat diet-induced NAFLD rats	77:108	high-fat diet-induced NAFLD rats	77:108	Effects of shenling baizhu powder herbal formula on intestinal microbiota in high-fat diet-induced NAFLD rats.
29710519	9	73	theme	total	1213:1217	arg1	TC					1232:1233	TC	1232:1233	TC	1232:1233	Serum biochemical analysis indicated that SLBZP decreased the serum level of total cholesterol (TC) and improved liver function.
29710519	9	73	theme	total	1213:1217	arg1	cholesterol					1219:1229	total cholesterol	1213:1229	total cholesterol (TC)	1213:1234	Serum biochemical analysis indicated that SLBZP decreased the serum level of total cholesterol (TC) and improved liver function.
29710519	1	74	theme	BACKGROUND	111:120	arg1	disease					159:165	non-alcoholic fatty liver disease	133:165	non-alcoholic fatty liver disease (NAFLD)	133:173	BACKGROUND Worldwide, non-alcoholic fatty liver disease (NAFLD) is a common chronic liver disease closely associated with obesity, diabetes and other metabolic diseases.
29710519	1	74	theme	BACKGROUND	111:120	arg1	Worldwide					122:130	BACKGROUND Worldwide	111:130	BACKGROUND Worldwide	111:130	BACKGROUND Worldwide, non-alcoholic fatty liver disease (NAFLD) is a common chronic liver disease closely associated with obesity, diabetes and other metabolic diseases.
29710519	1	74	theme	BACKGROUND	111:120	arg1	disease					201:207	a common chronic liver disease	178:207	a common chronic liver disease closely associated with obesity, diabetes and other metabolic diseases	178:278	BACKGROUND Worldwide, non-alcoholic fatty liver disease (NAFLD) is a common chronic liver disease closely associated with obesity, diabetes and other metabolic diseases.
29710519	6	75	theme	pathway	896:902	arg1	protein					912:918	liver TLR4 pathway related protein	885:918	liver TLR4 pathway related protein	885:918	The expression profiles of liver TLR4 pathway related protein was confirmed by western blotting.
29710519	4	76	theme	serum	675:679	arg1	indices					693:699	serum biochemical indices	675:699	serum biochemical indices	675:699	METHODS We established an NAFLD model in rats given a high-fat diet (HFD), administered different interventions and measured serum biochemical indices and inflammatory factors.
29710519	7	77	theme	microbiota	977:986	arg1	composition					988:998	intestinal microbiota composition	966:998	intestinal microbiota composition	966:998	Changes in intestinal microbiota composition were analyzed using a 16S rDNA sequencing technique.
29710519	14	78	theme	short-chain	1829:1839	arg1	SCFA					1853:1856	SCFA	1853:1856	SCFA	1853:1856	Moreover, SLBZP increased the relative abundance of short-chain fatty acid (SCFA)-producing bacteria, including Bifidobacterium and Anaerostipes.
29710519	14	78	theme	short-chain	1829:1839	arg1	acid					1847:1850	short-chain fatty acid	1829:1850	short-chain fatty acid (SCFA)-producing bacteria	1829:1876	Moreover, SLBZP increased the relative abundance of short-chain fatty acid (SCFA)-producing bacteria, including Bifidobacterium and Anaerostipes.
29710519	1	79	theme	common	180:185	arg1	Worldwide					122:130	BACKGROUND Worldwide	111:130	BACKGROUND Worldwide	111:130	BACKGROUND Worldwide, non-alcoholic fatty liver disease (NAFLD) is a common chronic liver disease closely associated with obesity, diabetes and other metabolic diseases.
29710519	1	79	theme	common	180:185	arg1	disease					201:207	a common chronic liver disease	178:207	a common chronic liver disease closely associated with obesity, diabetes and other metabolic diseases	178:278	BACKGROUND Worldwide, non-alcoholic fatty liver disease (NAFLD) is a common chronic liver disease closely associated with obesity, diabetes and other metabolic diseases.
29710519	1	80	theme	fatty	147:151	arg1	NAFLD					168:172	NAFLD	168:172	NAFLD	168:172	BACKGROUND Worldwide, non-alcoholic fatty liver disease (NAFLD) is a common chronic liver disease closely associated with obesity, diabetes and other metabolic diseases.
29710519	1	80	theme	fatty	147:151	arg1	disease					159:165	non-alcoholic fatty liver disease	133:165	non-alcoholic fatty liver disease (NAFLD)	133:173	BACKGROUND Worldwide, non-alcoholic fatty liver disease (NAFLD) is a common chronic liver disease closely associated with obesity, diabetes and other metabolic diseases.
29710519	1	80	theme	fatty	147:151	arg1	Worldwide					122:130	BACKGROUND Worldwide	111:130	BACKGROUND Worldwide	111:130	BACKGROUND Worldwide, non-alcoholic fatty liver disease (NAFLD) is a common chronic liver disease closely associated with obesity, diabetes and other metabolic diseases.
29710519	4	81	theme	different	638:646	arg1	diet					613:616	a high-fat diet	602:616	a high-fat diet (HFD)	602:622	METHODS We established an NAFLD model in rats given a high-fat diet (HFD), administered different interventions and measured serum biochemical indices and inflammatory factors.
29710519	4	81	theme	different	638:646	arg1	interventions					648:660	different interventions	638:660	administered different interventions	625:660	METHODS We established an NAFLD model in rats given a high-fat diet (HFD), administered different interventions and measured serum biochemical indices and inflammatory factors.
29710519	0	82	theme	high-fat	77:84	arg1	rats					105:108	high-fat diet-induced NAFLD rats	77:108	high-fat diet-induced NAFLD rats	77:108	Effects of shenling baizhu powder herbal formula on intestinal microbiota in high-fat diet-induced NAFLD rats.
29710519	12	83	theme	microbiota	1629:1638	arg1	abundance					1605:1613	the abundance	1601:1613	the abundance of intestinal microbiota	1601:1638	Microbiome analysis revealed that SLBZP improved the abundance of intestinal microbiota.
29710519	0	84	theme	NAFLD	99:103	arg1	rats					105:108	high-fat diet-induced NAFLD rats	77:108	high-fat diet-induced NAFLD rats	77:108	Effects of shenling baizhu powder herbal formula on intestinal microbiota in high-fat diet-induced NAFLD rats.
29710519	15	85	theme	increased	2034:2042	arg1	abundance					2044:2052	the increased abundance	2030:2052	the increased abundance of beneficial gut microbiota and decreased levels of LPS in the portal vein	2030:2128	CONCLUSION Taken together, these results suggest that the effects of SLBZP against NAFLD may be related to the increased abundance of beneficial gut microbiota and decreased levels of LPS in the portal vein.
29710519	3	86	contain	has	476:478	arg1	SLBZP					470:474	SLBZP	470:474	SLBZP	470:474	SLBZP has improved NAFLD symptoms; however, its mechanism of action is unknown.
29710519	3	86	contain	has	476:478	arg2	symptoms					495:502	improved NAFLD symptoms	480:502	improved NAFLD symptoms	480:502	SLBZP has improved NAFLD symptoms; however, its mechanism of action is unknown.
29710519	13	87	from	changes	1731:1737	arg1	composition					1764:1774	intestinal microbiota composition	1742:1774	intestinal microbiota composition	1742:1774	In taxonomy-based analysis, compared with control rats, SLBZP-treated rats showed obvious changes in intestinal microbiota composition.
29710519	15	88	theme	portal	2118:2123	arg1	vein					2125:2128	the portal vein	2114:2128	the portal vein	2114:2128	CONCLUSION Taken together, these results suggest that the effects of SLBZP against NAFLD may be related to the increased abundance of beneficial gut microbiota and decreased levels of LPS in the portal vein.
29710519	2	89	theme	plants	361:366	arg1	plants					361:366	natural medicinal plants	343:366	natural medicinal plants	343:366	Shenling Baizhu powder (SLBZP), a formulation of a variety of natural medicinal plants, has hepatoprotective properties and clinical efficacy in treating non-infectious intestinal disease.
29710519	2	89	theme	plants	361:366	arg1	variety					332:338	a variety	330:338	a variety of natural medicinal plants	330:366	Shenling Baizhu powder (SLBZP), a formulation of a variety of natural medicinal plants, has hepatoprotective properties and clinical efficacy in treating non-infectious intestinal disease.
29710519	13	90	theme	taxonomy-based	1644:1657	arg1	analysis					1659:1666	taxonomy-based analysis	1644:1666	taxonomy-based analysis	1644:1666	In taxonomy-based analysis, compared with control rats, SLBZP-treated rats showed obvious changes in intestinal microbiota composition.
29710519	4	91	theme	inflammatory	705:716	arg1	factors					718:724	inflammatory factors	705:724	inflammatory factors	705:724	METHODS We established an NAFLD model in rats given a high-fat diet (HFD), administered different interventions and measured serum biochemical indices and inflammatory factors.
29710519	2	92	theme	natural	343:349	arg1	plants					361:366	natural medicinal plants	343:366	natural medicinal plants	343:366	Shenling Baizhu powder (SLBZP), a formulation of a variety of natural medicinal plants, has hepatoprotective properties and clinical efficacy in treating non-infectious intestinal disease.
29710519	0	93	theme	baizhu	20:25	arg1	formula					41:47	shenling baizhu powder herbal formula	11:47	shenling baizhu powder herbal formula	11:47	Effects of shenling baizhu powder herbal formula on intestinal microbiota in high-fat diet-induced NAFLD rats.
29710519	2	94	theme	clinical	405:412	arg1	efficacy					414:421	clinical efficacy	405:421	clinical efficacy	405:421	Shenling Baizhu powder (SLBZP), a formulation of a variety of natural medicinal plants, has hepatoprotective properties and clinical efficacy in treating non-infectious intestinal disease.
29710519	0	95	theme	herbal	34:39	arg1	formula					41:47	shenling baizhu powder herbal formula	11:47	shenling baizhu powder herbal formula	11:47	Effects of shenling baizhu powder herbal formula on intestinal microbiota in high-fat diet-induced NAFLD rats.
29710519	15	96	theme	LPS	2107:2109	arg1	microbiota					2072:2081	beneficial gut microbiota	2057:2081	beneficial gut microbiota	2057:2081	CONCLUSION Taken together, these results suggest that the effects of SLBZP against NAFLD may be related to the increased abundance of beneficial gut microbiota and decreased levels of LPS in the portal vein.
29710519	15	96	theme	LPS	2107:2109	arg1	levels					2097:2102	decreased levels	2087:2102	decreased levels of LPS	2087:2109	CONCLUSION Taken together, these results suggest that the effects of SLBZP against NAFLD may be related to the increased abundance of beneficial gut microbiota and decreased levels of LPS in the portal vein.
29710519	15	97	theme	levels	2097:2102	arg1	abundance					2044:2052	the increased abundance	2030:2052	the increased abundance of beneficial gut microbiota and decreased levels of LPS in the portal vein	2030:2128	CONCLUSION Taken together, these results suggest that the effects of SLBZP against NAFLD may be related to the increased abundance of beneficial gut microbiota and decreased levels of LPS in the portal vein.
29710519	2	98	theme	non-infectious	435:448	arg1	disease					461:467	non-infectious intestinal disease	435:467	non-infectious intestinal disease	435:467	Shenling Baizhu powder (SLBZP), a formulation of a variety of natural medicinal plants, has hepatoprotective properties and clinical efficacy in treating non-infectious intestinal disease.
29710519	10	99	theme	related	1437:1443	arg1	protein					1445:1451	TLR4 pathway related protein	1424:1451	TLR4 pathway related protein	1424:1451	Additionally, SLBZP decreased the serum level of endotoxin, tumor necrosis factor α (TNF-α), interleukin-1β (IL-β) (p < 0.05), and decreased the expression of TLR4 pathway related protein.
29710519	3	100	theme	NAFLD	489:493	arg1	symptoms					495:502	improved NAFLD symptoms	480:502	improved NAFLD symptoms	480:502	SLBZP has improved NAFLD symptoms; however, its mechanism of action is unknown.
29710519	8	101	theme	body	1096:1099	arg1	weight					1101:1106	body weight	1096:1106	body weight	1096:1106	RESULTS Of note, SLBZP effectively reduced body weight in HFD-fed rats (p < 0.05).
29710519	15	102	theme	gut	2068:2070	arg1	microbiota					2072:2081	beneficial gut microbiota	2057:2081	beneficial gut microbiota	2057:2081	CONCLUSION Taken together, these results suggest that the effects of SLBZP against NAFLD may be related to the increased abundance of beneficial gut microbiota and decreased levels of LPS in the portal vein.
29710519	10	103	theme	TLR4	1424:1427	arg1	protein					1445:1451	TLR4 pathway related protein	1424:1451	TLR4 pathway related protein	1424:1451	Additionally, SLBZP decreased the serum level of endotoxin, tumor necrosis factor α (TNF-α), interleukin-1β (IL-β) (p < 0.05), and decreased the expression of TLR4 pathway related protein.
29710519	10	104	theme	tumor	1325:1329	arg1	endotoxin					1314:1322	endotoxin	1314:1322	endotoxin	1314:1322	Additionally, SLBZP decreased the serum level of endotoxin, tumor necrosis factor α (TNF-α), interleukin-1β (IL-β) (p < 0.05), and decreased the expression of TLR4 pathway related protein.
29710519	10	104	theme	tumor	1325:1329	arg1	factor					1340:1345	tumor necrosis factor α	1325:1347	tumor necrosis factor α (TNF-α)	1325:1355	Additionally, SLBZP decreased the serum level of endotoxin, tumor necrosis factor α (TNF-α), interleukin-1β (IL-β) (p < 0.05), and decreased the expression of TLR4 pathway related protein.
29710519	10	104	theme	tumor	1325:1329	arg1	TNF-α					1350:1354	TNF-α	1350:1354	TNF-α	1350:1354	Additionally, SLBZP decreased the serum level of endotoxin, tumor necrosis factor α (TNF-α), interleukin-1β (IL-β) (p < 0.05), and decreased the expression of TLR4 pathway related protein.
29710519	10	105	theme	endotoxin	1314:1322	arg1	level					1305:1309	the serum level	1295:1309	the serum level of endotoxin, tumor necrosis factor α (TNF-α), interleukin-1β (IL-β) (p < 0.05),	1295:1390	Additionally, SLBZP decreased the serum level of endotoxin, tumor necrosis factor α (TNF-α), interleukin-1β (IL-β) (p < 0.05), and decreased the expression of TLR4 pathway related protein.
29710519	2	106	theme	variety	332:338	arg1	powder					297:302	Shenling Baizhu powder	281:302	Shenling Baizhu powder (SLBZP)	281:310	Shenling Baizhu powder (SLBZP), a formulation of a variety of natural medicinal plants, has hepatoprotective properties and clinical efficacy in treating non-infectious intestinal disease.
29710519	2	106	theme	variety	332:338	arg1	formulation					315:325	a formulation	313:325	a formulation of a variety of natural medicinal plants	313:366	Shenling Baizhu powder (SLBZP), a formulation of a variety of natural medicinal plants, has hepatoprotective properties and clinical efficacy in treating non-infectious intestinal disease.
30143117	4	0	theme	gelation	668:675	arg1	time					677:680	appropriate gelation time	656:680	appropriate gelation time	656:680	The results gained from these studies show that this composite materials have a series of excellent performance such as appropriate gelation time, good swelling ability, suitable water evaporation rate, well hemocompatibility and biological compatibility.
30143117	1	1	theme	absorbent	167:175	arg1	capacity					177:184	great absorbent capacity	161:184	great absorbent capacity	161:184	Hydrogel wound dressing is a new type of biomaterial with great absorbent capacity, which has been extensively researched and its application in the field of biomedicine is common.
30143117	4	2	theme	appropriate	656:666	arg1	time					677:680	appropriate gelation time	656:680	appropriate gelation time	656:680	The results gained from these studies show that this composite materials have a series of excellent performance such as appropriate gelation time, good swelling ability, suitable water evaporation rate, well hemocompatibility and biological compatibility.
30143117	1	3	theme	biomedicine	261:271	arg1	field					252:256	the field	248:256	the field of biomedicine	248:271	Hydrogel wound dressing is a new type of biomaterial with great absorbent capacity, which has been extensively researched and its application in the field of biomedicine is common.
30143117	5	4	theme	moist	907:911	arg1	healing					919:925	a moist wound healing	905:925	a moist wound healing environment	905:937	In particular, the super absorbent capacity (the maximum swelling rate is 2888%), which is conducive to maintain a moist wound healing environment.
30143117	2	5	dep	acid	357:360	arg1	both					401:404	both	401:404	both	401:404	In this study, we prepared a novel composite hydrogel made of hyaluronic acid (HA) and hydroxyethyl cellulose (HEC), both of them are natural polysaccharides.
30143117	4	6	theme	evaporation	721:731	arg1	rate					733:736	suitable water evaporation rate	706:736	suitable water evaporation rate	706:736	The results gained from these studies show that this composite materials have a series of excellent performance such as appropriate gelation time, good swelling ability, suitable water evaporation rate, well hemocompatibility and biological compatibility.
30143117	2	7	theme	hyaluronic	346:355	arg1	HA					363:364	HA	363:364	HA	363:364	In this study, we prepared a novel composite hydrogel made of hyaluronic acid (HA) and hydroxyethyl cellulose (HEC), both of them are natural polysaccharides.
30143117	2	7	theme	hyaluronic	346:355	arg1	acid					357:360	hyaluronic acid	346:360	hyaluronic acid (HA)	346:365	In this study, we prepared a novel composite hydrogel made of hyaluronic acid (HA) and hydroxyethyl cellulose (HEC), both of them are natural polysaccharides.
30143117	2	8	theme	novel	313:317	arg1	hydrogel					329:336	a novel composite hydrogel	311:336	a novel composite hydrogel made of hyaluronic acid (HA) and hydroxyethyl cellulose (HEC), both of them are natural polysaccharides	311:440	In this study, we prepared a novel composite hydrogel made of hyaluronic acid (HA) and hydroxyethyl cellulose (HEC), both of them are natural polysaccharides.
30143117	5	9	theme	super	811:815	arg1	conducive					883:891	conducive	883:891	conducive	883:891	In particular, the super absorbent capacity (the maximum swelling rate is 2888%), which is conducive to maintain a moist wound healing environment.
30143117	5	9	theme	super	811:815	arg1	capacity					827:834	the super absorbent capacity	807:834	the super absorbent capacity (the maximum swelling rate is 2888%)	807:871	In particular, the super absorbent capacity (the maximum swelling rate is 2888%), which is conducive to maintain a moist wound healing environment.
30143117	3	10	theme	composite	486:494	arg1	hydrogel					496:503	this composite hydrogel	481:503	this composite hydrogel	481:503	Then, the structure and properties of this composite hydrogel were characterized and tested.
30143117	5	11	theme	maximum	841:847	arg1	rate					858:861	the maximum swelling rate	837:861	the maximum swelling rate	837:861	In particular, the super absorbent capacity (the maximum swelling rate is 2888%), which is conducive to maintain a moist wound healing environment.
30143117	5	11	theme	maximum	841:847	arg1	%					870:870	2888%	866:870	2888%	866:870	In particular, the super absorbent capacity (the maximum swelling rate is 2888%), which is conducive to maintain a moist wound healing environment.
30143117	2	12	theme	hydroxyethyl	371:382	arg1	HEC					395:397	HEC	395:397	HEC	395:397	In this study, we prepared a novel composite hydrogel made of hyaluronic acid (HA) and hydroxyethyl cellulose (HEC), both of them are natural polysaccharides.
30143117	2	12	theme	hydroxyethyl	371:382	arg1	cellulose					384:392	hydroxyethyl cellulose	371:392	hydroxyethyl cellulose (HEC)	371:398	In this study, we prepared a novel composite hydrogel made of hyaluronic acid (HA) and hydroxyethyl cellulose (HEC), both of them are natural polysaccharides.
30143117	1	13	with	biomaterial	144:154	arg1	capacity					177:184	great absorbent capacity	161:184	great absorbent capacity	161:184	Hydrogel wound dressing is a new type of biomaterial with great absorbent capacity, which has been extensively researched and its application in the field of biomedicine is common.
30143117	1	14	theme	Hydrogel	103:110	arg1	dressing					118:125	Hydrogel wound dressing	103:125	Hydrogel wound dressing	103:125	Hydrogel wound dressing is a new type of biomaterial with great absorbent capacity, which has been extensively researched and its application in the field of biomedicine is common.
30143117	1	14	theme	Hydrogel	103:110	arg1	type					136:139	a new type	130:139	a new type	130:139	Hydrogel wound dressing is a new type of biomaterial with great absorbent capacity, which has been extensively researched and its application in the field of biomedicine is common.
30143117	2	15	dep	them	409:412	arg1	polysaccharides					426:440	natural polysaccharides	418:440	natural polysaccharides	418:440	In this study, we prepared a novel composite hydrogel made of hyaluronic acid (HA) and hydroxyethyl cellulose (HEC), both of them are natural polysaccharides.
30143117	3	16	dep	structure	453:461	arg1	the					449:451	the	449:451	the	449:451	Then, the structure and properties of this composite hydrogel were characterized and tested.
30143117	1	17	theme	wound	112:116	arg1	dressing					118:125	Hydrogel wound dressing	103:125	Hydrogel wound dressing	103:125	Hydrogel wound dressing is a new type of biomaterial with great absorbent capacity, which has been extensively researched and its application in the field of biomedicine is common.
30143117	1	17	theme	wound	112:116	arg1	type					136:139	a new type	130:139	a new type	130:139	Hydrogel wound dressing is a new type of biomaterial with great absorbent capacity, which has been extensively researched and its application in the field of biomedicine is common.
30143117	4	18	theme	performance	636:646	arg1	series					616:621	a series	614:621	a series of excellent performance such as appropriate gelation time, good swelling ability, suitable water evaporation rate, well hemocompatibility and biological compatibility	614:789	The results gained from these studies show that this composite materials have a series of excellent performance such as appropriate gelation time, good swelling ability, suitable water evaporation rate, well hemocompatibility and biological compatibility.
30143117	4	19	theme	good	683:686	arg1	ability					697:703	good swelling ability	683:703	good swelling ability	683:703	The results gained from these studies show that this composite materials have a series of excellent performance such as appropriate gelation time, good swelling ability, suitable water evaporation rate, well hemocompatibility and biological compatibility.
30143117	4	20	theme	excellent	626:634	arg1	performance					636:646	excellent performance	626:646	excellent performance such as appropriate gelation time, good swelling ability, suitable water evaporation rate, well hemocompatibility and biological compatibility	626:789	The results gained from these studies show that this composite materials have a series of excellent performance such as appropriate gelation time, good swelling ability, suitable water evaporation rate, well hemocompatibility and biological compatibility.
30143117	6	21	used	used	997:1000	arg2	hydrogel					981:988	this hydrogel	976:988	this hydrogel	976:988	Taking account of these properties, this hydrogel can be used clinically as a wound dressing.
30143117	6	21	used	used	997:1000	arg2	dressing					1024:1031	a wound dressing	1016:1031	a wound dressing	1016:1031	Taking account of these properties, this hydrogel can be used clinically as a wound dressing.
30143117	4	22	theme	water	715:719	arg1	rate					733:736	suitable water evaporation rate	706:736	suitable water evaporation rate	706:736	The results gained from these studies show that this composite materials have a series of excellent performance such as appropriate gelation time, good swelling ability, suitable water evaporation rate, well hemocompatibility and biological compatibility.
30143117	4	23	theme	well	739:742	arg1	hemocompatibility					744:760	well hemocompatibility	739:760	well hemocompatibility	739:760	The results gained from these studies show that this composite materials have a series of excellent performance such as appropriate gelation time, good swelling ability, suitable water evaporation rate, well hemocompatibility and biological compatibility.
30143117	4	24	contain	have	609:612	arg1	materials					599:607	this composite materials	584:607	this composite materials	584:607	The results gained from these studies show that this composite materials have a series of excellent performance such as appropriate gelation time, good swelling ability, suitable water evaporation rate, well hemocompatibility and biological compatibility.
30143117	4	24	contain	have	609:612	arg2	series					616:621	a series	614:621	a series of excellent performance such as appropriate gelation time, good swelling ability, suitable water evaporation rate, well hemocompatibility and biological compatibility	614:789	The results gained from these studies show that this composite materials have a series of excellent performance such as appropriate gelation time, good swelling ability, suitable water evaporation rate, well hemocompatibility and biological compatibility.
30143117	4	25	theme	suitable	706:713	arg1	rate					733:736	suitable water evaporation rate	706:736	suitable water evaporation rate	706:736	The results gained from these studies show that this composite materials have a series of excellent performance such as appropriate gelation time, good swelling ability, suitable water evaporation rate, well hemocompatibility and biological compatibility.
30143117	4	26	theme	composite	589:597	arg1	materials					599:607	this composite materials	584:607	this composite materials	584:607	The results gained from these studies show that this composite materials have a series of excellent performance such as appropriate gelation time, good swelling ability, suitable water evaporation rate, well hemocompatibility and biological compatibility.
30143117	0	27	theme	hyaluronic	45:54	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of aminated hyaluronic acid/oxidized hydroxyethyl cellulose hydrogel.
30143117	0	27	theme	hyaluronic	45:54	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of aminated hyaluronic acid/oxidized hydroxyethyl cellulose hydrogel.
30143117	5	28	theme	swelling	849:856	arg1	rate					858:861	the maximum swelling rate	837:861	the maximum swelling rate	837:861	In particular, the super absorbent capacity (the maximum swelling rate is 2888%), which is conducive to maintain a moist wound healing environment.
30143117	5	28	theme	swelling	849:856	arg1	%					870:870	2888%	866:870	2888%	866:870	In particular, the super absorbent capacity (the maximum swelling rate is 2888%), which is conducive to maintain a moist wound healing environment.
30143117	5	29	theme	absorbent	817:825	arg1	conducive					883:891	conducive	883:891	conducive	883:891	In particular, the super absorbent capacity (the maximum swelling rate is 2888%), which is conducive to maintain a moist wound healing environment.
30143117	5	29	theme	absorbent	817:825	arg1	capacity					827:834	the super absorbent capacity	807:834	the super absorbent capacity (the maximum swelling rate is 2888%)	807:871	In particular, the super absorbent capacity (the maximum swelling rate is 2888%), which is conducive to maintain a moist wound healing environment.
30143117	6	30	theme	wound	1018:1022	arg1	dressing					1024:1031	a wound dressing	1016:1031	a wound dressing	1016:1031	Taking account of these properties, this hydrogel can be used clinically as a wound dressing.
30143117	6	30	theme	wound	1018:1022	arg1	hydrogel					981:988	this hydrogel	976:988	this hydrogel	976:988	Taking account of these properties, this hydrogel can be used clinically as a wound dressing.
30143117	2	31	theme	natural	418:424	arg1	polysaccharides					426:440	natural polysaccharides	418:440	natural polysaccharides	418:440	In this study, we prepared a novel composite hydrogel made of hyaluronic acid (HA) and hydroxyethyl cellulose (HEC), both of them are natural polysaccharides.
30143117	0	32	theme	cellulose	83:91	arg1	hydrogel					93:100	hydroxyethyl cellulose hydrogel	70:100	hydroxyethyl cellulose hydrogel	70:100	Preparation and characterization of aminated hyaluronic acid/oxidized hydroxyethyl cellulose hydrogel.
30143117	5	33	theme	wound	913:917	arg1	healing					919:925	a moist wound healing	905:925	a moist wound healing environment	905:937	In particular, the super absorbent capacity (the maximum swelling rate is 2888%), which is conducive to maintain a moist wound healing environment.
30143117	1	34	theme	new	132:134	arg1	dressing					118:125	Hydrogel wound dressing	103:125	Hydrogel wound dressing	103:125	Hydrogel wound dressing is a new type of biomaterial with great absorbent capacity, which has been extensively researched and its application in the field of biomedicine is common.
30143117	1	34	theme	new	132:134	arg1	type					136:139	a new type	130:139	a new type	130:139	Hydrogel wound dressing is a new type of biomaterial with great absorbent capacity, which has been extensively researched and its application in the field of biomedicine is common.
30143117	1	35	from	application	233:243	arg1	field					252:256	the field	248:256	the field of biomedicine	248:271	Hydrogel wound dressing is a new type of biomaterial with great absorbent capacity, which has been extensively researched and its application in the field of biomedicine is common.
30143117	2	36	theme	composite	319:327	arg1	hydrogel					329:336	a novel composite hydrogel	311:336	a novel composite hydrogel made of hyaluronic acid (HA) and hydroxyethyl cellulose (HEC), both of them are natural polysaccharides	311:440	In this study, we prepared a novel composite hydrogel made of hyaluronic acid (HA) and hydroxyethyl cellulose (HEC), both of them are natural polysaccharides.
30143117	3	37	theme	hydrogel	496:503	arg1	properties					467:476	properties	467:476	properties	467:476	Then, the structure and properties of this composite hydrogel were characterized and tested.
30143117	3	37	theme	hydrogel	496:503	arg1	structure					453:461	structure	453:461	structure	453:461	Then, the structure and properties of this composite hydrogel were characterized and tested.
30143117	5	38	dep	capacity	827:834	arg1	rate					858:861	the maximum swelling rate	837:861	the maximum swelling rate	837:861	In particular, the super absorbent capacity (the maximum swelling rate is 2888%), which is conducive to maintain a moist wound healing environment.
30143117	5	38	dep	capacity	827:834	arg1	%					870:870	2888%	866:870	2888%	866:870	In particular, the super absorbent capacity (the maximum swelling rate is 2888%), which is conducive to maintain a moist wound healing environment.
30143117	1	39	theme	biomaterial	144:154	arg1	dressing					118:125	Hydrogel wound dressing	103:125	Hydrogel wound dressing	103:125	Hydrogel wound dressing is a new type of biomaterial with great absorbent capacity, which has been extensively researched and its application in the field of biomedicine is common.
30143117	1	39	theme	biomaterial	144:154	arg1	type					136:139	a new type	130:139	a new type	130:139	Hydrogel wound dressing is a new type of biomaterial with great absorbent capacity, which has been extensively researched and its application in the field of biomedicine is common.
30143117	4	40	theme	swelling	688:695	arg1	ability					697:703	good swelling ability	683:703	good swelling ability	683:703	The results gained from these studies show that this composite materials have a series of excellent performance such as appropriate gelation time, good swelling ability, suitable water evaporation rate, well hemocompatibility and biological compatibility.
30143117	5	41	theme	healing	919:925	arg1	environment					927:937	a moist wound healing environment	905:937	a moist wound healing environment	905:937	In particular, the super absorbent capacity (the maximum swelling rate is 2888%), which is conducive to maintain a moist wound healing environment.
30143117	6	42	theme	properties	964:973	arg1	account					947:953	account	947:953	account of these properties	947:973	Taking account of these properties, this hydrogel can be used clinically as a wound dressing.
30143117	0	43	theme	hydroxyethyl	70:81	arg1	hydrogel					93:100	hydroxyethyl cellulose hydrogel	70:100	hydroxyethyl cellulose hydrogel	70:100	Preparation and characterization of aminated hyaluronic acid/oxidized hydroxyethyl cellulose hydrogel.
30143117	4	44	theme	biological	766:775	arg1	compatibility					777:789	biological compatibility	766:789	biological compatibility	766:789	The results gained from these studies show that this composite materials have a series of excellent performance such as appropriate gelation time, good swelling ability, suitable water evaporation rate, well hemocompatibility and biological compatibility.
30143117	1	45	theme	great	161:165	arg1	capacity					177:184	great absorbent capacity	161:184	great absorbent capacity	161:184	Hydrogel wound dressing is a new type of biomaterial with great absorbent capacity, which has been extensively researched and its application in the field of biomedicine is common.
29753720	5	0	theme	electrochemical	781:795	arg1	EIS					821:823	EIS	821:823	EIS	821:823	The electrochemical performances were investigated by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV).
29753720	5	0	theme	electrochemical	781:795	arg1	spectroscopy					807:818	electrochemical impedance spectroscopy	781:818	electrochemical impedance spectroscopy (EIS)	781:824	The electrochemical performances were investigated by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV).
29753720	1	1	theme	paracetamol	321:331	arg1	determination					304:316	the quantitative determination	287:316	the quantitative determination of paracetamol (PR)	287:336	In the present study, a nanocomposite of f-MWCNTs-chitosan-Co was prepared by the immobilization of Co(II) on f-MWCNTs-chitosan by a self-assembly method and used for the quantitative determination of paracetamol (PR).
29753720	7	2	theme	fabricated	1064:1073	arg1	sensor					1075:1080	The fabricated sensor	1060:1080	The fabricated sensor	1060:1080	The fabricated sensor exhibited significant selectivity towards PR detection.
29753720	0	3	theme	functionalized	76:89	arg1	MWCNTs					91:96	functionalized MWCNTs	76:96	functionalized MWCNTs	76:96	Enhanced amperometric detection of paracetamol by immobilized cobalt ion on functionalized MWCNTs - Chitosan thin film.
29753720	6	4	theme	0.01 μmol L-1	1025:1037	arg1	limit					1016:1020	a detection limit	1004:1020	a detection limit of 0.01 μmol L-1 for the PR solution	1004:1057	Under favorable experimental conditions, differential pulse voltammetry showed a linear dynamic range between 0.1 and 400 μmol L-1 with a detection limit of 0.01 μmol L-1 for the PR solution.
29753720	0	5	theme	Chitosan	100:107	arg1	film					114:117	Chitosan thin film	100:117	Chitosan thin film	100:117	Enhanced amperometric detection of paracetamol by immobilized cobalt ion on functionalized MWCNTs - Chitosan thin film.
29753720	8	6	theme	PR	1210:1211	arg1	determination					1193:1205	the determination	1189:1205	the determination of PR in commercial tablets and human serum sample	1189:1256	The fabricated sensor was successfully applied for the determination of PR in commercial tablets and human serum sample.
29753720	1	7	used	used	278:281	arg2	nanocomposite					144:156	a nanocomposite	142:156	a nanocomposite of f-MWCNTs-chitosan-Co	142:180	In the present study, a nanocomposite of f-MWCNTs-chitosan-Co was prepared by the immobilization of Co(II) on f-MWCNTs-chitosan by a self-assembly method and used for the quantitative determination of paracetamol (PR).
29753720	1	7	used	used	278:281	arg2	f-MWCNTs-chitosan-Co					161:180	f-MWCNTs-chitosan-Co	161:180	f-MWCNTs-chitosan-Co	161:180	In the present study, a nanocomposite of f-MWCNTs-chitosan-Co was prepared by the immobilization of Co(II) on f-MWCNTs-chitosan by a self-assembly method and used for the quantitative determination of paracetamol (PR).
29753720	8	8	theme	human	1239:1243	arg1	sample					1251:1256	human serum sample	1239:1256	human serum sample	1239:1256	The fabricated sensor was successfully applied for the determination of PR in commercial tablets and human serum sample.
29753720	6	9	theme	pulse	922:926	arg1	voltammetry					928:938	differential pulse voltammetry	909:938	differential pulse voltammetry	909:938	Under favorable experimental conditions, differential pulse voltammetry showed a linear dynamic range between 0.1 and 400 μmol L-1 with a detection limit of 0.01 μmol L-1 for the PR solution.
29753720	2	10	theme	field	374:378	arg1	FESEM					419:423	FESEM	419:423	FESEM	419:423	The composite was characterized by field emission scanning electron microscopy (FESEM) and energy dispersive x-ray analysis (EDX).
29753720	2	10	theme	field	374:378	arg1	microscopy					407:416	field emission scanning electron microscopy	374:416	field emission scanning electron microscopy (FESEM)	374:424	The composite was characterized by field emission scanning electron microscopy (FESEM) and energy dispersive x-ray analysis (EDX).
29753720	0	11	theme	thin	109:112	arg1	film					114:117	Chitosan thin film	100:117	Chitosan thin film	100:117	Enhanced amperometric detection of paracetamol by immobilized cobalt ion on functionalized MWCNTs - Chitosan thin film.
29753720	6	12	theme	differential	909:920	arg1	voltammetry					928:938	differential pulse voltammetry	909:938	differential pulse voltammetry	909:938	Under favorable experimental conditions, differential pulse voltammetry showed a linear dynamic range between 0.1 and 400 μmol L-1 with a detection limit of 0.01 μmol L-1 for the PR solution.
29753720	7	13	theme	PR	1124:1125	arg1	detection					1127:1135	PR detection	1124:1135	PR detection	1124:1135	The fabricated sensor exhibited significant selectivity towards PR detection.
29753720	5	14	theme	differential	830:841	arg1	DPV					862:864	DPV	862:864	DPV	862:864	The electrochemical performances were investigated by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV).
29753720	5	14	theme	differential	830:841	arg1	voltammetry					849:859	differential pulse voltammetry	830:859	differential pulse voltammetry (DPV)	830:865	The electrochemical performances were investigated by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV).
29753720	1	15	theme	f-MWCNTs-chitosan-Co	161:180	arg1	f-MWCNTs-chitosan-Co					161:180	f-MWCNTs-chitosan-Co	161:180	f-MWCNTs-chitosan-Co	161:180	In the present study, a nanocomposite of f-MWCNTs-chitosan-Co was prepared by the immobilization of Co(II) on f-MWCNTs-chitosan by a self-assembly method and used for the quantitative determination of paracetamol (PR).
29753720	1	15	theme	f-MWCNTs-chitosan-Co	161:180	arg1	nanocomposite					144:156	a nanocomposite	142:156	a nanocomposite of f-MWCNTs-chitosan-Co	142:180	In the present study, a nanocomposite of f-MWCNTs-chitosan-Co was prepared by the immobilization of Co(II) on f-MWCNTs-chitosan by a self-assembly method and used for the quantitative determination of paracetamol (PR).
29753720	0	16	theme	amperometric	9:20	arg1	detection					22:30	Enhanced amperometric detection	0:30	Enhanced amperometric detection of paracetamol by immobilized cobalt ion on functionalized MWCNTs - Chitosan thin film.	0:118	Enhanced amperometric detection of paracetamol by immobilized cobalt ion on functionalized MWCNTs - Chitosan thin film.
29753720	5	17	theme	impedance	797:805	arg1	EIS					821:823	EIS	821:823	EIS	821:823	The electrochemical performances were investigated by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV).
29753720	5	17	theme	impedance	797:805	arg1	spectroscopy					807:818	electrochemical impedance spectroscopy	781:818	electrochemical impedance spectroscopy (EIS)	781:824	The electrochemical performances were investigated by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV).
29753720	6	18	theme	detection	1006:1014	arg1	limit					1016:1020	a detection limit	1004:1020	a detection limit of 0.01 μmol L-1 for the PR solution	1004:1057	Under favorable experimental conditions, differential pulse voltammetry showed a linear dynamic range between 0.1 and 400 μmol L-1 with a detection limit of 0.01 μmol L-1 for the PR solution.
29753720	0	19	theme	Enhanced	0:7	arg1	detection					22:30	Enhanced amperometric detection	0:30	Enhanced amperometric detection of paracetamol by immobilized cobalt ion on functionalized MWCNTs - Chitosan thin film.	0:118	Enhanced amperometric detection of paracetamol by immobilized cobalt ion on functionalized MWCNTs - Chitosan thin film.
29753720	1	20	theme	self-assembly	253:265	arg1	method					267:272	a self-assembly method	251:272	a self-assembly method	251:272	In the present study, a nanocomposite of f-MWCNTs-chitosan-Co was prepared by the immobilization of Co(II) on f-MWCNTs-chitosan by a self-assembly method and used for the quantitative determination of paracetamol (PR).
29753720	8	21	from	determination	1193:1205	arg1	sample					1251:1256	human serum sample	1239:1256	human serum sample	1239:1256	The fabricated sensor was successfully applied for the determination of PR in commercial tablets and human serum sample.
29753720	8	21	from	determination	1193:1205	arg1	tablets					1227:1233	commercial tablets	1216:1233	commercial tablets	1216:1233	The fabricated sensor was successfully applied for the determination of PR in commercial tablets and human serum sample.
29753720	5	22	theme	pulse	843:847	arg1	DPV					862:864	DPV	862:864	DPV	862:864	The electrochemical performances were investigated by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV).
29753720	5	22	theme	pulse	843:847	arg1	voltammetry					849:859	differential pulse voltammetry	830:859	differential pulse voltammetry (DPV)	830:865	The electrochemical performances were investigated by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV).
29753720	8	23	theme	commercial	1216:1225	arg1	tablets					1227:1233	commercial tablets	1216:1233	commercial tablets	1216:1233	The fabricated sensor was successfully applied for the determination of PR in commercial tablets and human serum sample.
29753720	4	24	theme	strong	640:645	arg1	activity					664:671	strong electrocatalytic activity	640:671	strong electrocatalytic activity towards the oxidation of PR	640:699	The prepared GCE modified f-MWCNTs/CTS-Co showed strong electrocatalytic activity towards the oxidation of PR.
29753720	8	25	theme	serum	1245:1249	arg1	sample					1251:1256	human serum sample	1239:1256	human serum sample	1239:1256	The fabricated sensor was successfully applied for the determination of PR in commercial tablets and human serum sample.
29753720	4	26	theme	GCE	604:606	arg1	f-MWCNTs/CTS-Co					617:631	The prepared GCE modified f-MWCNTs/CTS-Co	591:631	The prepared GCE modified f-MWCNTs/CTS-Co	591:631	The prepared GCE modified f-MWCNTs/CTS-Co showed strong electrocatalytic activity towards the oxidation of PR.
29753720	6	27	theme	linear	949:954	arg1	range					964:968	a linear dynamic range	947:968	a linear dynamic range between 0.1 and 400 μmol L-1	947:997	Under favorable experimental conditions, differential pulse voltammetry showed a linear dynamic range between 0.1 and 400 μmol L-1 with a detection limit of 0.01 μmol L-1 for the PR solution.
29753720	2	28	theme	electron	398:405	arg1	FESEM					419:423	FESEM	419:423	FESEM	419:423	The composite was characterized by field emission scanning electron microscopy (FESEM) and energy dispersive x-ray analysis (EDX).
29753720	2	28	theme	electron	398:405	arg1	microscopy					407:416	field emission scanning electron microscopy	374:416	field emission scanning electron microscopy (FESEM)	374:424	The composite was characterized by field emission scanning electron microscopy (FESEM) and energy dispersive x-ray analysis (EDX).
29753720	7	29	theme	significant	1092:1102	arg1	selectivity					1104:1114	significant selectivity	1092:1114	significant selectivity towards PR detection	1092:1135	The fabricated sensor exhibited significant selectivity towards PR detection.
29753720	6	30	theme	experimental	884:895	arg1	conditions					897:906	favorable experimental conditions	874:906	favorable experimental conditions	874:906	Under favorable experimental conditions, differential pulse voltammetry showed a linear dynamic range between 0.1 and 400 μmol L-1 with a detection limit of 0.01 μmol L-1 for the PR solution.
29753720	2	31	theme	scanning	389:396	arg1	FESEM					419:423	FESEM	419:423	FESEM	419:423	The composite was characterized by field emission scanning electron microscopy (FESEM) and energy dispersive x-ray analysis (EDX).
29753720	2	31	theme	scanning	389:396	arg1	microscopy					407:416	field emission scanning electron microscopy	374:416	field emission scanning electron microscopy (FESEM)	374:424	The composite was characterized by field emission scanning electron microscopy (FESEM) and energy dispersive x-ray analysis (EDX).
29753720	0	32	theme	paracetamol	35:45	arg1	detection					22:30	Enhanced amperometric detection	0:30	Enhanced amperometric detection of paracetamol by immobilized cobalt ion on functionalized MWCNTs - Chitosan thin film.	0:118	Enhanced amperometric detection of paracetamol by immobilized cobalt ion on functionalized MWCNTs - Chitosan thin film.
29753720	2	33	theme	x-ray	448:452	arg1	EDX					464:466	EDX	464:466	EDX	464:466	The composite was characterized by field emission scanning electron microscopy (FESEM) and energy dispersive x-ray analysis (EDX).
29753720	2	33	theme	x-ray	448:452	arg1	analysis					454:461	energy dispersive x-ray analysis	430:461	energy dispersive x-ray analysis (EDX)	430:467	The composite was characterized by field emission scanning electron microscopy (FESEM) and energy dispersive x-ray analysis (EDX).
29753720	6	34	theme	favorable	874:882	arg1	conditions					897:906	favorable experimental conditions	874:906	favorable experimental conditions	874:906	Under favorable experimental conditions, differential pulse voltammetry showed a linear dynamic range between 0.1 and 400 μmol L-1 with a detection limit of 0.01 μmol L-1 for the PR solution.
29753720	1	35	from	immobilization	202:215	arg1	f-MWCNTs-chitosan					230:246	f-MWCNTs-chitosan	230:246	f-MWCNTs-chitosan	230:246	In the present study, a nanocomposite of f-MWCNTs-chitosan-Co was prepared by the immobilization of Co(II) on f-MWCNTs-chitosan by a self-assembly method and used for the quantitative determination of paracetamol (PR).
29753720	2	36	theme	emission	380:387	arg1	FESEM					419:423	FESEM	419:423	FESEM	419:423	The composite was characterized by field emission scanning electron microscopy (FESEM) and energy dispersive x-ray analysis (EDX).
29753720	2	36	theme	emission	380:387	arg1	microscopy					407:416	field emission scanning electron microscopy	374:416	field emission scanning electron microscopy (FESEM)	374:424	The composite was characterized by field emission scanning electron microscopy (FESEM) and energy dispersive x-ray analysis (EDX).
29753720	0	37	theme	cobalt	62:67	arg1	ion					69:71	immobilized cobalt ion	50:71	immobilized cobalt ion on functionalized MWCNTs	50:96	Enhanced amperometric detection of paracetamol by immobilized cobalt ion on functionalized MWCNTs - Chitosan thin film.
29753720	5	38	theme	cyclic	756:761	arg1	CV					776:777	CV	776:777	CV	776:777	The electrochemical performances were investigated by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV).
29753720	5	38	theme	cyclic	756:761	arg1	voltammetry					763:773	cyclic voltammetry	756:773	cyclic voltammetry (CV)	756:778	The electrochemical performances were investigated by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV).
29753720	0	39	from	ion	69:71	arg1	MWCNTs					91:96	functionalized MWCNTs	76:96	functionalized MWCNTs	76:96	Enhanced amperometric detection of paracetamol by immobilized cobalt ion on functionalized MWCNTs - Chitosan thin film.
29753720	0	40	theme	immobilized	50:60	arg1	ion					69:71	immobilized cobalt ion	50:71	immobilized cobalt ion on functionalized MWCNTs	50:96	Enhanced amperometric detection of paracetamol by immobilized cobalt ion on functionalized MWCNTs - Chitosan thin film.
29753720	1	41	theme	quantitative	291:302	arg1	determination					304:316	the quantitative determination	287:316	the quantitative determination of paracetamol (PR)	287:336	In the present study, a nanocomposite of f-MWCNTs-chitosan-Co was prepared by the immobilization of Co(II) on f-MWCNTs-chitosan by a self-assembly method and used for the quantitative determination of paracetamol (PR).
29753720	2	42	theme	energy	430:435	arg1	EDX					464:466	EDX	464:466	EDX	464:466	The composite was characterized by field emission scanning electron microscopy (FESEM) and energy dispersive x-ray analysis (EDX).
29753720	2	42	theme	energy	430:435	arg1	analysis					454:461	energy dispersive x-ray analysis	430:461	energy dispersive x-ray analysis (EDX)	430:467	The composite was characterized by field emission scanning electron microscopy (FESEM) and energy dispersive x-ray analysis (EDX).
29753720	3	43	theme	paracetamol	578:588	arg1	electro-oxidation					557:573	the electro-oxidation	553:573	the electro-oxidation of paracetamol	553:588	The electroactivity of cobalt immobilized on f-MWCNTs-chitosan was assessed during the electro-oxidation of paracetamol.
29753720	8	44	theme	fabricated	1142:1151	arg1	sensor					1153:1158	The fabricated sensor	1138:1158	The fabricated sensor	1138:1158	The fabricated sensor was successfully applied for the determination of PR in commercial tablets and human serum sample.
29753720	4	45	theme	PR	698:699	arg1	oxidation					685:693	the oxidation	681:693	the oxidation of PR	681:699	The prepared GCE modified f-MWCNTs/CTS-Co showed strong electrocatalytic activity towards the oxidation of PR.
29753720	1	46	theme	Co	220:221	arg1	immobilization					202:215	the immobilization	198:215	the immobilization of Co(II) on f-MWCNTs-chitosan	198:246	In the present study, a nanocomposite of f-MWCNTs-chitosan-Co was prepared by the immobilization of Co(II) on f-MWCNTs-chitosan by a self-assembly method and used for the quantitative determination of paracetamol (PR).
29753720	5	47	theme	electrochemical	706:720	arg1	performances					722:733	The electrochemical performances	702:733	The electrochemical performances	702:733	The electrochemical performances were investigated by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV).
29753720	1	48	theme	present	127:133	arg1	study					135:139	the present study	123:139	the present study	123:139	In the present study, a nanocomposite of f-MWCNTs-chitosan-Co was prepared by the immobilization of Co(II) on f-MWCNTs-chitosan by a self-assembly method and used for the quantitative determination of paracetamol (PR).
29753720	4	49	theme	prepared	595:602	arg1	f-MWCNTs/CTS-Co					617:631	The prepared GCE modified f-MWCNTs/CTS-Co	591:631	The prepared GCE modified f-MWCNTs/CTS-Co	591:631	The prepared GCE modified f-MWCNTs/CTS-Co showed strong electrocatalytic activity towards the oxidation of PR.
29753720	4	50	theme	electrocatalytic	647:662	arg1	activity					664:671	strong electrocatalytic activity	640:671	strong electrocatalytic activity towards the oxidation of PR	640:699	The prepared GCE modified f-MWCNTs/CTS-Co showed strong electrocatalytic activity towards the oxidation of PR.
29753720	6	51	theme	PR	1047:1048	arg1	solution					1050:1057	the PR solution	1043:1057	the PR solution	1043:1057	Under favorable experimental conditions, differential pulse voltammetry showed a linear dynamic range between 0.1 and 400 μmol L-1 with a detection limit of 0.01 μmol L-1 for the PR solution.
29753720	2	52	theme	dispersive	437:446	arg1	EDX					464:466	EDX	464:466	EDX	464:466	The composite was characterized by field emission scanning electron microscopy (FESEM) and energy dispersive x-ray analysis (EDX).
29753720	2	52	theme	dispersive	437:446	arg1	analysis					454:461	energy dispersive x-ray analysis	430:461	energy dispersive x-ray analysis (EDX)	430:467	The composite was characterized by field emission scanning electron microscopy (FESEM) and energy dispersive x-ray analysis (EDX).
29753720	6	53	theme	dynamic	956:962	arg1	range					964:968	a linear dynamic range	947:968	a linear dynamic range between 0.1 and 400 μmol L-1	947:997	Under favorable experimental conditions, differential pulse voltammetry showed a linear dynamic range between 0.1 and 400 μmol L-1 with a detection limit of 0.01 μmol L-1 for the PR solution.
29753720	3	54	theme	cobalt	493:498	arg1	electroactivity					474:488	The electroactivity	470:488	The electroactivity of cobalt immobilized on f-MWCNTs-chitosan	470:531	The electroactivity of cobalt immobilized on f-MWCNTs-chitosan was assessed during the electro-oxidation of paracetamol.
29753720	4	55	theme	modified	608:615	arg1	f-MWCNTs/CTS-Co					617:631	The prepared GCE modified f-MWCNTs/CTS-Co	591:631	The prepared GCE modified f-MWCNTs/CTS-Co	591:631	The prepared GCE modified f-MWCNTs/CTS-Co showed strong electrocatalytic activity towards the oxidation of PR.
30584946	0	0	theme	activity	90:97	arg1	Enhancement					61:71	Enhancement	61:71	Development of chitosan-PEG blended films using Trichoderma: Enhancement of antimicrobial activity and seed quality.	0:115	Development of chitosan-PEG blended films using Trichoderma: Enhancement of antimicrobial activity and seed quality.
30584946	1	1	theme	bioagent	349:356	arg1	wastage					358:364	bioagent wastage	349:364	bioagent wastage	349:364	Fungi under genus Trichoderma as ameliorates of biotic and abiotic stresses in cultivated crops is gaining popularity world-wide and their application in conjunction with seed coating polymers is an attractive proposition to reduce bioagent wastage and harnessing benefits of combined application.
30584946	7	2	theme	optimized	1007:1015	arg1	solution					1022:1029	The optimized film solution	1003:1029	The optimized film solution in combination with different Trichoderma strains	1003:1079	The optimized film solution in combination with different Trichoderma strains improved seed quality parameters in test crop castor (Ricinus communis).
30584946	3	3	theme	blended	537:543	arg1	solutions					550:558	blended film solutions	537:558	blended film solutions	537:558	A series of blended film solutions were synthesized by using chitosan, PEG and plasticizer in varying concentrations.
30584946	9	4	theme	chitosan-PEG-Th	1322:1336	arg1	blend					1338:1342	the optimized chitosan-PEG-Th blend	1308:1342	the optimized chitosan-PEG-Th blend	1308:1342	In conclusion, the optimized chitosan-PEG-Th blend was effective in enhancing seed germination and plant growth of castor.
30584946	9	4	theme	chitosan-PEG-Th	1322:1336	arg1	effective					1348:1356	effective	1348:1356	effective	1348:1356	In conclusion, the optimized chitosan-PEG-Th blend was effective in enhancing seed germination and plant growth of castor.
30584946	5	5	from	Studies	747:753	arg1	compatibility					758:770	compatibility	758:770	compatibility of film forming ingredients with Trichoderma	758:815	Studies on compatibility of film forming ingredients with Trichoderma have not shown any inhibition and antimicrobial activity has shown different levels of inhibition of plant pathogens.
30584946	0	6	theme	antimicrobial	76:88	arg1	activity					90:97	antimicrobial activity	76:97	antimicrobial activity	76:97	Development of chitosan-PEG blended films using Trichoderma: Enhancement of antimicrobial activity and seed quality.
30584946	10	7	theme	field	1463:1467	arg1	evaluation					1469:1478	large field evaluation	1457:1478	large field evaluation	1457:1478	The material can be further tested under large field evaluation as a seed coating agent against various plant diseases.
30584946	1	8	theme	biotic	165:170	arg1	stresses					184:191	biotic and abiotic stresses	165:191	biotic and abiotic stresses in cultivated crops	165:211	Fungi under genus Trichoderma as ameliorates of biotic and abiotic stresses in cultivated crops is gaining popularity world-wide and their application in conjunction with seed coating polymers is an attractive proposition to reduce bioagent wastage and harnessing benefits of combined application.
30584946	7	9	from	parameters	1103:1112	arg1	castor					1127:1132	test crop castor	1117:1132	test crop castor (Ricinus communis)	1117:1151	The optimized film solution in combination with different Trichoderma strains improved seed quality parameters in test crop castor (Ricinus communis).
30584946	0	10	theme	seed	103:106	arg1	quality					108:114	seed quality	103:114	seed quality	103:114	Development of chitosan-PEG blended films using Trichoderma: Enhancement of antimicrobial activity and seed quality.
30584946	9	11	theme	optimized	1312:1320	arg1	blend					1338:1342	the optimized chitosan-PEG-Th blend	1308:1342	the optimized chitosan-PEG-Th blend	1308:1342	In conclusion, the optimized chitosan-PEG-Th blend was effective in enhancing seed germination and plant growth of castor.
30584946	9	11	theme	optimized	1312:1320	arg1	effective					1348:1356	effective	1348:1356	effective	1348:1356	In conclusion, the optimized chitosan-PEG-Th blend was effective in enhancing seed germination and plant growth of castor.
30584946	10	12	theme	large	1457:1461	arg1	evaluation					1469:1478	large field evaluation	1457:1478	large field evaluation	1457:1478	The material can be further tested under large field evaluation as a seed coating agent against various plant diseases.
30584946	3	13	from	chitosan	586:593	arg1	concentrations					627:640	varying concentrations	619:640	varying concentrations	619:640	A series of blended film solutions were synthesized by using chitosan, PEG and plasticizer in varying concentrations.
30584946	7	14	theme	film	1017:1020	arg1	solution					1022:1029	The optimized film solution	1003:1029	The optimized film solution in combination with different Trichoderma strains	1003:1079	The optimized film solution in combination with different Trichoderma strains improved seed quality parameters in test crop castor (Ricinus communis).
30584946	1	15	dep	proposition	327:337	arg1	reduce					342:347	reduce	342:347	to reduce bioagent wastage	339:364	Fungi under genus Trichoderma as ameliorates of biotic and abiotic stresses in cultivated crops is gaining popularity world-wide and their application in conjunction with seed coating polymers is an attractive proposition to reduce bioagent wastage and harnessing benefits of combined application.
30584946	1	15	dep	proposition	327:337	arg1	harnessing					370:379	harnessing	370:379	harnessing benefits of combined application	370:412	Fungi under genus Trichoderma as ameliorates of biotic and abiotic stresses in cultivated crops is gaining popularity world-wide and their application in conjunction with seed coating polymers is an attractive proposition to reduce bioagent wastage and harnessing benefits of combined application.
30584946	4	16	theme	seed	733:736	arg1	coating					738:744	seed coating	733:744	seed coating	733:744	The optimization of blended film composition and dose for coating of seeds was done w.r.t seed coating.
30584946	5	17	theme	inhibition	904:913	arg1	levels					894:899	different levels	884:899	different levels of inhibition of plant pathogens	884:932	Studies on compatibility of film forming ingredients with Trichoderma have not shown any inhibition and antimicrobial activity has shown different levels of inhibition of plant pathogens.
30584946	5	18	theme	film	775:778	arg1	ingredients					788:798	film forming ingredients	775:798	film forming ingredients with Trichoderma	775:815	Studies on compatibility of film forming ingredients with Trichoderma have not shown any inhibition and antimicrobial activity has shown different levels of inhibition of plant pathogens.
30584946	2	19	with	action	431:436	arg1	polymers					466:473	natural polymers	458:473	natural polymers like chitosan	458:487	The synergistic action of Trichoderma with natural polymers like chitosan can enhance antimicrobial activity.
30584946	5	20	theme	different	884:892	arg1	levels					894:899	different levels	884:899	different levels of inhibition of plant pathogens	884:932	Studies on compatibility of film forming ingredients with Trichoderma have not shown any inhibition and antimicrobial activity has shown different levels of inhibition of plant pathogens.
30584946	4	21	theme	film	671:674	arg1	composition					676:686	blended film composition	663:686	blended film composition	663:686	The optimization of blended film composition and dose for coating of seeds was done w.r.t seed coating.
30584946	5	22	theme	plant	918:922	arg1	pathogens					924:932	plant pathogens	918:932	plant pathogens	918:932	Studies on compatibility of film forming ingredients with Trichoderma have not shown any inhibition and antimicrobial activity has shown different levels of inhibition of plant pathogens.
30584946	2	23	theme	synergistic	419:429	arg1	action					431:436	The synergistic action	415:436	The synergistic action of Trichoderma with natural polymers like chitosan	415:487	The synergistic action of Trichoderma with natural polymers like chitosan can enhance antimicrobial activity.
30584946	0	24	theme	quality	108:114	arg1	Enhancement					61:71	Enhancement	61:71	Development of chitosan-PEG blended films using Trichoderma: Enhancement of antimicrobial activity and seed quality.	0:115	Development of chitosan-PEG blended films using Trichoderma: Enhancement of antimicrobial activity and seed quality.
30584946	10	25	theme	seed	1485:1488	arg1	agent					1498:1502	a seed coating agent	1483:1502	a seed coating agent against various plant diseases	1483:1533	The material can be further tested under large field evaluation as a seed coating agent against various plant diseases.
30584946	10	25	theme	seed	1485:1488	arg1	material					1420:1427	The material	1416:1427	The material	1416:1427	The material can be further tested under large field evaluation as a seed coating agent against various plant diseases.
30584946	4	26	theme	composition	676:686	arg1	optimization					647:658	The optimization	643:658	The optimization of blended film composition and dose for coating of seeds	643:716	The optimization of blended film composition and dose for coating of seeds was done w.r.t seed coating.
30584946	9	27	from	effective	1348:1356	arg1	conclusion					1296:1305	conclusion	1296:1305	conclusion	1296:1305	In conclusion, the optimized chitosan-PEG-Th blend was effective in enhancing seed germination and plant growth of castor.
30584946	1	28	theme	abiotic	176:182	arg1	stresses					184:191	biotic and abiotic stresses	165:191	biotic and abiotic stresses in cultivated crops	165:211	Fungi under genus Trichoderma as ameliorates of biotic and abiotic stresses in cultivated crops is gaining popularity world-wide and their application in conjunction with seed coating polymers is an attractive proposition to reduce bioagent wastage and harnessing benefits of combined application.
30584946	1	29	theme	combined	393:400	arg1	application					402:412	combined application	393:412	combined application	393:412	Fungi under genus Trichoderma as ameliorates of biotic and abiotic stresses in cultivated crops is gaining popularity world-wide and their application in conjunction with seed coating polymers is an attractive proposition to reduce bioagent wastage and harnessing benefits of combined application.
30584946	7	30	theme	crop	1122:1125	arg1	castor					1127:1132	test crop castor	1117:1132	test crop castor (Ricinus communis)	1117:1151	The optimized film solution in combination with different Trichoderma strains improved seed quality parameters in test crop castor (Ricinus communis).
30584946	4	31	theme	seeds	712:716	arg1	coating					701:707	coating	701:707	coating of seeds	701:716	The optimization of blended film composition and dose for coating of seeds was done w.r.t seed coating.
30584946	5	32	theme	antimicrobial	851:863	arg1	activity					865:872	antimicrobial activity	851:872	antimicrobial activity	851:872	Studies on compatibility of film forming ingredients with Trichoderma have not shown any inhibition and antimicrobial activity has shown different levels of inhibition of plant pathogens.
30584946	1	33	theme	seed	288:291	arg1	polymers					301:308	seed coating polymers	288:308	seed coating polymers	288:308	Fungi under genus Trichoderma as ameliorates of biotic and abiotic stresses in cultivated crops is gaining popularity world-wide and their application in conjunction with seed coating polymers is an attractive proposition to reduce bioagent wastage and harnessing benefits of combined application.
30584946	8	34	theme	combination	1261:1271	arg1	formulation					1273:1283	Th4d combination formulation	1256:1283	Th4d combination formulation (3023)	1256:1290	Significant increase in vigour index (3110) was observed with Th4d treatment followed by chitosan and Th4d combination formulation (3023).
30584946	8	34	theme	combination	1261:1271	arg1	3023					1286:1289	3023	1286:1289	3023	1286:1289	Significant increase in vigour index (3110) was observed with Th4d treatment followed by chitosan and Th4d combination formulation (3023).
30584946	10	35	theme	coating	1490:1496	arg1	agent					1498:1502	a seed coating agent	1483:1502	a seed coating agent against various plant diseases	1483:1533	The material can be further tested under large field evaluation as a seed coating agent against various plant diseases.
30584946	10	35	theme	coating	1490:1496	arg1	material					1420:1427	The material	1416:1427	The material	1416:1427	The material can be further tested under large field evaluation as a seed coating agent against various plant diseases.
30584946	8	36	from	increase	1166:1173	arg1	3110					1192:1195	3110	1192:1195	3110	1192:1195	Significant increase in vigour index (3110) was observed with Th4d treatment followed by chitosan and Th4d combination formulation (3023).
30584946	8	36	from	increase	1166:1173	arg1	index					1185:1189	vigour index	1178:1189	vigour index (3110)	1178:1196	Significant increase in vigour index (3110) was observed with Th4d treatment followed by chitosan and Th4d combination formulation (3023).
30584946	5	37	theme	pathogens	924:932	arg1	inhibition					904:913	inhibition	904:913	inhibition of plant pathogens	904:932	Studies on compatibility of film forming ingredients with Trichoderma have not shown any inhibition and antimicrobial activity has shown different levels of inhibition of plant pathogens.
30584946	0	38	theme	blended	28:34	arg1	films					36:40	chitosan-PEG blended films	15:40	chitosan-PEG blended films using Trichoderma	15:58	Development of chitosan-PEG blended films using Trichoderma: Enhancement of antimicrobial activity and seed quality.
30584946	1	39	theme	coating	293:299	arg1	polymers					301:308	seed coating polymers	288:308	seed coating polymers	288:308	Fungi under genus Trichoderma as ameliorates of biotic and abiotic stresses in cultivated crops is gaining popularity world-wide and their application in conjunction with seed coating polymers is an attractive proposition to reduce bioagent wastage and harnessing benefits of combined application.
30584946	10	40	theme	plant	1520:1524	arg1	diseases					1526:1533	various plant diseases	1512:1533	various plant diseases	1512:1533	The material can be further tested under large field evaluation as a seed coating agent against various plant diseases.
30584946	1	41	theme	cultivated	196:205	arg1	crops					207:211	cultivated crops	196:211	cultivated crops	196:211	Fungi under genus Trichoderma as ameliorates of biotic and abiotic stresses in cultivated crops is gaining popularity world-wide and their application in conjunction with seed coating polymers is an attractive proposition to reduce bioagent wastage and harnessing benefits of combined application.
30584946	0	42	theme	chitosan-PEG	15:26	arg1	films					36:40	chitosan-PEG blended films	15:40	chitosan-PEG blended films using Trichoderma	15:58	Development of chitosan-PEG blended films using Trichoderma: Enhancement of antimicrobial activity and seed quality.
30584946	7	43	theme	quality	1095:1101	arg1	parameters					1103:1112	seed quality parameters	1090:1112	seed quality parameters in test crop castor (Ricinus communis)	1090:1151	The optimized film solution in combination with different Trichoderma strains improved seed quality parameters in test crop castor (Ricinus communis).
30584946	7	44	theme	test	1117:1120	arg1	castor					1127:1132	test crop castor	1117:1132	test crop castor (Ricinus communis)	1117:1151	The optimized film solution in combination with different Trichoderma strains improved seed quality parameters in test crop castor (Ricinus communis).
30584946	1	45	theme	application	402:412	arg1	benefits					381:388	benefits	381:388	benefits of combined application	381:412	Fungi under genus Trichoderma as ameliorates of biotic and abiotic stresses in cultivated crops is gaining popularity world-wide and their application in conjunction with seed coating polymers is an attractive proposition to reduce bioagent wastage and harnessing benefits of combined application.
30584946	3	46	theme	film	545:548	arg1	solutions					550:558	blended film solutions	537:558	blended film solutions	537:558	A series of blended film solutions were synthesized by using chitosan, PEG and plasticizer in varying concentrations.
30584946	8	47	theme	vigour	1178:1183	arg1	3110					1192:1195	3110	1192:1195	3110	1192:1195	Significant increase in vigour index (3110) was observed with Th4d treatment followed by chitosan and Th4d combination formulation (3023).
30584946	8	47	theme	vigour	1178:1183	arg1	index					1185:1189	vigour index	1178:1189	vigour index (3110)	1178:1196	Significant increase in vigour index (3110) was observed with Th4d treatment followed by chitosan and Th4d combination formulation (3023).
30584946	2	48	theme	Trichoderma	441:451	arg1	action					431:436	The synergistic action	415:436	The synergistic action of Trichoderma with natural polymers like chitosan	415:487	The synergistic action of Trichoderma with natural polymers like chitosan can enhance antimicrobial activity.
30584946	1	49	from	application	256:266	arg1	conjunction					271:281	conjunction	271:281	conjunction with seed coating polymers	271:308	Fungi under genus Trichoderma as ameliorates of biotic and abiotic stresses in cultivated crops is gaining popularity world-wide and their application in conjunction with seed coating polymers is an attractive proposition to reduce bioagent wastage and harnessing benefits of combined application.
30584946	7	50	from	solution	1022:1029	arg1	combination					1034:1044	combination	1034:1044	combination with different Trichoderma strains	1034:1079	The optimized film solution in combination with different Trichoderma strains improved seed quality parameters in test crop castor (Ricinus communis).
30584946	4	51	theme	dose	692:695	arg1	optimization					647:658	The optimization	643:658	The optimization of blended film composition and dose for coating of seeds	643:716	The optimization of blended film composition and dose for coating of seeds was done w.r.t seed coating.
30584946	0	52	theme	films	36:40	arg1	Development					0:10	Development	0:10	Development of chitosan-PEG blended films using Trichoderma: Enhancement of antimicrobial activity and seed quality.	0:115	Development of chitosan-PEG blended films using Trichoderma: Enhancement of antimicrobial activity and seed quality.
30584946	2	53	theme	antimicrobial	501:513	arg1	activity					515:522	antimicrobial activity	501:522	antimicrobial activity	501:522	The synergistic action of Trichoderma with natural polymers like chitosan can enhance antimicrobial activity.
30584946	7	54	with	combination	1034:1044	arg1	strains					1073:1079	different Trichoderma strains	1051:1079	different Trichoderma strains	1051:1079	The optimized film solution in combination with different Trichoderma strains improved seed quality parameters in test crop castor (Ricinus communis).
30584946	1	55	with	conjunction	271:281	arg1	polymers					301:308	seed coating polymers	288:308	seed coating polymers	288:308	Fungi under genus Trichoderma as ameliorates of biotic and abiotic stresses in cultivated crops is gaining popularity world-wide and their application in conjunction with seed coating polymers is an attractive proposition to reduce bioagent wastage and harnessing benefits of combined application.
30584946	5	56	theme	forming	780:786	arg1	ingredients					788:798	film forming ingredients	775:798	film forming ingredients with Trichoderma	775:815	Studies on compatibility of film forming ingredients with Trichoderma have not shown any inhibition and antimicrobial activity has shown different levels of inhibition of plant pathogens.
30584946	9	57	from	conclusion	1296:1305	arg1	blend					1338:1342	the optimized chitosan-PEG-Th blend	1308:1342	the optimized chitosan-PEG-Th blend	1308:1342	In conclusion, the optimized chitosan-PEG-Th blend was effective in enhancing seed germination and plant growth of castor.
30584946	9	57	from	conclusion	1296:1305	arg1	effective					1348:1356	effective	1348:1356	effective	1348:1356	In conclusion, the optimized chitosan-PEG-Th blend was effective in enhancing seed germination and plant growth of castor.
30584946	1	58	theme	attractive	316:325	arg1	proposition					327:337	an attractive proposition	313:337	an attractive proposition to reduce bioagent wastage and harnessing benefits of combined application	313:412	Fungi under genus Trichoderma as ameliorates of biotic and abiotic stresses in cultivated crops is gaining popularity world-wide and their application in conjunction with seed coating polymers is an attractive proposition to reduce bioagent wastage and harnessing benefits of combined application.
30584946	1	58	theme	attractive	316:325	arg1	application					256:266	their application	250:266	their application in conjunction with seed coating polymers	250:308	Fungi under genus Trichoderma as ameliorates of biotic and abiotic stresses in cultivated crops is gaining popularity world-wide and their application in conjunction with seed coating polymers is an attractive proposition to reduce bioagent wastage and harnessing benefits of combined application.
30584946	7	59	dep	castor	1127:1132	arg1	communis					1143:1150	Ricinus communis	1135:1150	Ricinus communis	1135:1150	The optimized film solution in combination with different Trichoderma strains improved seed quality parameters in test crop castor (Ricinus communis).
30584946	7	60	theme	different	1051:1059	arg1	strains					1073:1079	different Trichoderma strains	1051:1079	different Trichoderma strains	1051:1079	The optimized film solution in combination with different Trichoderma strains improved seed quality parameters in test crop castor (Ricinus communis).
30584946	7	61	theme	seed	1090:1093	arg1	parameters					1103:1112	seed quality parameters	1090:1112	seed quality parameters in test crop castor (Ricinus communis)	1090:1151	The optimized film solution in combination with different Trichoderma strains improved seed quality parameters in test crop castor (Ricinus communis).
30584946	3	62	theme	varying	619:625	arg1	concentrations					627:640	varying concentrations	619:640	varying concentrations	619:640	A series of blended film solutions were synthesized by using chitosan, PEG and plasticizer in varying concentrations.
30584946	7	63	theme	Trichoderma	1061:1071	arg1	strains					1073:1079	different Trichoderma strains	1051:1079	different Trichoderma strains	1051:1079	The optimized film solution in combination with different Trichoderma strains improved seed quality parameters in test crop castor (Ricinus communis).
30584946	8	64	theme	Th4d	1216:1219	arg1	treatment					1221:1229	Th4d treatment	1216:1229	Th4d treatment followed by chitosan and Th4d combination formulation (3023)	1216:1290	Significant increase in vigour index (3110) was observed with Th4d treatment followed by chitosan and Th4d combination formulation (3023).
30584946	8	65	theme	Significant	1154:1164	arg1	increase					1166:1173	Significant increase	1154:1173	Significant increase in vigour index (3110)	1154:1196	Significant increase in vigour index (3110) was observed with Th4d treatment followed by chitosan and Th4d combination formulation (3023).
30584946	9	66	theme	seed	1371:1374	arg1	germination					1376:1386	seed germination	1371:1386	seed germination	1371:1386	In conclusion, the optimized chitosan-PEG-Th blend was effective in enhancing seed germination and plant growth of castor.
30584946	10	67	theme	various	1512:1518	arg1	diseases					1526:1533	various plant diseases	1512:1533	various plant diseases	1512:1533	The material can be further tested under large field evaluation as a seed coating agent against various plant diseases.
30584946	3	68	from	PEG	596:598	arg1	concentrations					627:640	varying concentrations	619:640	varying concentrations	619:640	A series of blended film solutions were synthesized by using chitosan, PEG and plasticizer in varying concentrations.
30584946	1	69	theme	popularity	224:233	arg1	world-wide					235:244	popularity world-wide	224:244	popularity world-wide	224:244	Fungi under genus Trichoderma as ameliorates of biotic and abiotic stresses in cultivated crops is gaining popularity world-wide and their application in conjunction with seed coating polymers is an attractive proposition to reduce bioagent wastage and harnessing benefits of combined application.
30584946	1	70	from	stresses	184:191	arg1	crops					207:211	cultivated crops	196:211	cultivated crops	196:211	Fungi under genus Trichoderma as ameliorates of biotic and abiotic stresses in cultivated crops is gaining popularity world-wide and their application in conjunction with seed coating polymers is an attractive proposition to reduce bioagent wastage and harnessing benefits of combined application.
30584946	5	71	theme	ingredients	788:798	arg1	compatibility					758:770	compatibility	758:770	compatibility of film forming ingredients with Trichoderma	758:815	Studies on compatibility of film forming ingredients with Trichoderma have not shown any inhibition and antimicrobial activity has shown different levels of inhibition of plant pathogens.
30584946	5	72	with	ingredients	788:798	arg1	Trichoderma					805:815	Trichoderma	805:815	Trichoderma	805:815	Studies on compatibility of film forming ingredients with Trichoderma have not shown any inhibition and antimicrobial activity has shown different levels of inhibition of plant pathogens.
30584946	2	73	theme	natural	458:464	arg1	polymers					466:473	natural polymers	458:473	natural polymers like chitosan	458:487	The synergistic action of Trichoderma with natural polymers like chitosan can enhance antimicrobial activity.
30584946	3	74	theme	solutions	550:558	arg1	series					527:532	A series	525:532	A series of blended film solutions	525:558	A series of blended film solutions were synthesized by using chitosan, PEG and plasticizer in varying concentrations.
30584946	3	75	from	plasticizer	604:614	arg1	concentrations					627:640	varying concentrations	619:640	varying concentrations	619:640	A series of blended film solutions were synthesized by using chitosan, PEG and plasticizer in varying concentrations.
30584946	4	76	theme	blended	663:669	arg1	composition					676:686	blended film composition	663:686	blended film composition	663:686	The optimization of blended film composition and dose for coating of seeds was done w.r.t seed coating.
30584946	8	77	theme	Th4d	1256:1259	arg1	formulation					1273:1283	Th4d combination formulation	1256:1283	Th4d combination formulation (3023)	1256:1290	Significant increase in vigour index (3110) was observed with Th4d treatment followed by chitosan and Th4d combination formulation (3023).
30584946	8	77	theme	Th4d	1256:1259	arg1	3023					1286:1289	3023	1286:1289	3023	1286:1289	Significant increase in vigour index (3110) was observed with Th4d treatment followed by chitosan and Th4d combination formulation (3023).
30584946	0	78	dep	Development	0:10	arg1	Enhancement					61:71	Enhancement	61:71	Development of chitosan-PEG blended films using Trichoderma: Enhancement of antimicrobial activity and seed quality.	0:115	Development of chitosan-PEG blended films using Trichoderma: Enhancement of antimicrobial activity and seed quality.
30584946	9	79	theme	castor	1408:1413	arg1	germination					1376:1386	seed germination	1371:1386	seed germination	1371:1386	In conclusion, the optimized chitosan-PEG-Th blend was effective in enhancing seed germination and plant growth of castor.
30584946	9	79	theme	castor	1408:1413	arg1	growth					1398:1403	plant growth	1392:1403	plant growth	1392:1403	In conclusion, the optimized chitosan-PEG-Th blend was effective in enhancing seed germination and plant growth of castor.
30584946	9	80	theme	plant	1392:1396	arg1	growth					1398:1403	plant growth	1392:1403	plant growth	1392:1403	In conclusion, the optimized chitosan-PEG-Th blend was effective in enhancing seed germination and plant growth of castor.
29471162	6	0	theme	H2O2	1068:1071	arg1	concentrations					1073:1086	Boosting luminal H2O2 concentrations	1051:1086	Boosting luminal H2O2 concentrations within a physiological range	1051:1115	Boosting luminal H2O2 concentrations within a physiological range accelerated recovery from colitis, while significantly exceeding this H2O2 level triggered bacteraemia.
29471162	4	1	theme	defining	729:736	arg1	features					738:745	the defining features	725:745	the defining features of lactobacilli	725:761	Here we probe if one of the defining features of lactobacilli, the ability to generate nanomolar H2O2, contributes to their beneficial role in colitis.
29471162	5	2	theme	source	964:969	arg1	s					971:971	the enzymatic H2O2 source(s)	945:972	the enzymatic H2O2 source(s) followed by orally administering the bacteria before and during DSS colitis	945:1048	H2O2 generation by wild type L. johnsonii was modified by either deleting or overexpressing the enzymatic H2O2 source(s) followed by orally administering the bacteria before and during DSS colitis.
29471162	2	3	theme	tissue	306:311	arg1	restitution					313:323	tissue restitution	306:323	tissue restitution	306:323	Treatments for accelerating tissue restitution and prolonging remission are scarce, but altering the microbiota composition to promote intestinal homeostasis is considered a safe, economic and promising approach.
29471162	1	4	dep	disease	167:173	arg1	phases					270:275	phases	270:275	phases	270:275	Inflammatory bowel disease (IBD) is a multifactorial chronic inflammatory disease of the gastrointestinal tract, characterized by cycles of acute flares, recovery and remission phases.
29471162	4	5	theme	nanomolar	788:796	arg1	H2O2					798:801	nanomolar H2O2	788:801	nanomolar H2O2	788:801	Here we probe if one of the defining features of lactobacilli, the ability to generate nanomolar H2O2, contributes to their beneficial role in colitis.
29471162	4	6	theme	lactobacilli	750:761	arg1	features					738:745	the defining features	725:745	the defining features of lactobacilli	725:761	Here we probe if one of the defining features of lactobacilli, the ability to generate nanomolar H2O2, contributes to their beneficial role in colitis.
29471162	3	7	from	promise	554:560	arg1	trials					574:579	clinical trials	565:579	clinical trials	565:579	Although probiotic bacteria have not yet fulfilled fully their promise in clinical trials, understanding the mechanism of how they exert beneficial effects will permit devising improved therapeutic strategies.
29471162	5	8	theme	enzymatic	949:957	arg1	s					971:971	the enzymatic H2O2 source(s)	945:972	the enzymatic H2O2 source(s) followed by orally administering the bacteria before and during DSS colitis	945:1048	H2O2 generation by wild type L. johnsonii was modified by either deleting or overexpressing the enzymatic H2O2 source(s) followed by orally administering the bacteria before and during DSS colitis.
29471162	5	9	theme	L.	882:883	arg1	type					877:880	wild type L. johnsonii	872:893	wild type L. johnsonii	872:893	H2O2 generation by wild type L. johnsonii was modified by either deleting or overexpressing the enzymatic H2O2 source(s) followed by orally administering the bacteria before and during DSS colitis.
29471162	1	10	theme	Inflammatory	93:104	arg1	disease					112:118	Inflammatory bowel disease	93:118	Inflammatory bowel disease (IBD)	93:124	Inflammatory bowel disease (IBD) is a multifactorial chronic inflammatory disease of the gastrointestinal tract, characterized by cycles of acute flares, recovery and remission phases.
29471162	1	10	theme	Inflammatory	93:104	arg1	disease					167:173	a multifactorial chronic inflammatory disease	129:173	a multifactorial chronic inflammatory disease	129:173	Inflammatory bowel disease (IBD) is a multifactorial chronic inflammatory disease of the gastrointestinal tract, characterized by cycles of acute flares, recovery and remission phases.
29471162	1	10	theme	Inflammatory	93:104	arg1	IBD					121:123	IBD	121:123	IBD	121:123	Inflammatory bowel disease (IBD) is a multifactorial chronic inflammatory disease of the gastrointestinal tract, characterized by cycles of acute flares, recovery and remission phases.
29471162	1	11	theme	gastrointestinal	182:197	arg1	tract					199:203	the gastrointestinal tract	178:203	the gastrointestinal tract	178:203	Inflammatory bowel disease (IBD) is a multifactorial chronic inflammatory disease of the gastrointestinal tract, characterized by cycles of acute flares, recovery and remission phases.
29471162	2	12	theme	promising	471:479	arg1	approach					481:488	a safe, economic and promising approach	450:488	a safe, economic and promising approach	450:488	Treatments for accelerating tissue restitution and prolonging remission are scarce, but altering the microbiota composition to promote intestinal homeostasis is considered a safe, economic and promising approach.
29471162	7	13	from	barrier	1317:1323	arg1	range					1293:1297	the physiological range	1275:1297	the physiological range at the epithelial barrier	1275:1323	This study supports a role for increasing H2O2 within the physiological range at the epithelial barrier, independently of the enzymatic source and/or delivery mechanism, for inducing recovery and remission in IBD.
29471162	1	14	theme	bowel	106:110	arg1	disease					112:118	Inflammatory bowel disease	93:118	Inflammatory bowel disease (IBD)	93:124	Inflammatory bowel disease (IBD) is a multifactorial chronic inflammatory disease of the gastrointestinal tract, characterized by cycles of acute flares, recovery and remission phases.
29471162	1	14	theme	bowel	106:110	arg1	disease					167:173	a multifactorial chronic inflammatory disease	129:173	a multifactorial chronic inflammatory disease	129:173	Inflammatory bowel disease (IBD) is a multifactorial chronic inflammatory disease of the gastrointestinal tract, characterized by cycles of acute flares, recovery and remission phases.
29471162	1	14	theme	bowel	106:110	arg1	IBD					121:123	IBD	121:123	IBD	121:123	Inflammatory bowel disease (IBD) is a multifactorial chronic inflammatory disease of the gastrointestinal tract, characterized by cycles of acute flares, recovery and remission phases.
29471162	1	15	theme	tract	199:203	arg1	remission					260:268	remission	260:268	remission	260:268	Inflammatory bowel disease (IBD) is a multifactorial chronic inflammatory disease of the gastrointestinal tract, characterized by cycles of acute flares, recovery and remission phases.
29471162	1	15	theme	tract	199:203	arg1	recovery					247:254	recovery	247:254	recovery	247:254	Inflammatory bowel disease (IBD) is a multifactorial chronic inflammatory disease of the gastrointestinal tract, characterized by cycles of acute flares, recovery and remission phases.
29471162	1	15	theme	tract	199:203	arg1	disease					167:173	a multifactorial chronic inflammatory disease	129:173	a multifactorial chronic inflammatory disease	129:173	Inflammatory bowel disease (IBD) is a multifactorial chronic inflammatory disease of the gastrointestinal tract, characterized by cycles of acute flares, recovery and remission phases.
29471162	1	15	theme	tract	199:203	arg1	disease					112:118	Inflammatory bowel disease	93:118	Inflammatory bowel disease (IBD)	93:124	Inflammatory bowel disease (IBD) is a multifactorial chronic inflammatory disease of the gastrointestinal tract, characterized by cycles of acute flares, recovery and remission phases.
29471162	0	16	theme	Hydrogen	0:7	arg1	peroxide					9:16	Hydrogen peroxide	0:16	Hydrogen peroxide production by lactobacilli	0:43	Hydrogen peroxide production by lactobacilli promotes epithelial restitution during colitis.
29471162	7	17	theme	delivery	1371:1378	arg1	mechanism					1380:1388	delivery mechanism	1371:1388	delivery mechanism	1371:1388	This study supports a role for increasing H2O2 within the physiological range at the epithelial barrier, independently of the enzymatic source and/or delivery mechanism, for inducing recovery and remission in IBD.
29471162	2	18	theme	economic	458:465	arg1	approach					481:488	a safe, economic and promising approach	450:488	a safe, economic and promising approach	450:488	Treatments for accelerating tissue restitution and prolonging remission are scarce, but altering the microbiota composition to promote intestinal homeostasis is considered a safe, economic and promising approach.
29471162	6	19	theme	physiological	1097:1109	arg1	range					1111:1115	a physiological range	1095:1115	a physiological range	1095:1115	Boosting luminal H2O2 concentrations within a physiological range accelerated recovery from colitis, while significantly exceeding this H2O2 level triggered bacteraemia.
29471162	5	20	theme	H2O2	959:962	arg1	s					971:971	the enzymatic H2O2 source(s)	945:972	the enzymatic H2O2 source(s) followed by orally administering the bacteria before and during DSS colitis	945:1048	H2O2 generation by wild type L. johnsonii was modified by either deleting or overexpressing the enzymatic H2O2 source(s) followed by orally administering the bacteria before and during DSS colitis.
29471162	6	21	theme	H2O2	1187:1190	arg1	level					1192:1196	this H2O2 level	1182:1196	this H2O2 level triggered bacteraemia	1182:1218	Boosting luminal H2O2 concentrations within a physiological range accelerated recovery from colitis, while significantly exceeding this H2O2 level triggered bacteraemia.
29471162	2	22	theme	safe	452:455	arg1	approach					481:488	a safe, economic and promising approach	450:488	a safe, economic and promising approach	450:488	Treatments for accelerating tissue restitution and prolonging remission are scarce, but altering the microbiota composition to promote intestinal homeostasis is considered a safe, economic and promising approach.
29471162	5	23	theme	H2O2	853:856	arg1	generation					858:867	H2O2 generation	853:867	H2O2 generation by wild type L. johnsonii	853:893	H2O2 generation by wild type L. johnsonii was modified by either deleting or overexpressing the enzymatic H2O2 source(s) followed by orally administering the bacteria before and during DSS colitis.
29471162	3	24	theme	probiotic	500:508	arg1	bacteria					510:517	probiotic bacteria	500:517	probiotic bacteria	500:517	Although probiotic bacteria have not yet fulfilled fully their promise in clinical trials, understanding the mechanism of how they exert beneficial effects will permit devising improved therapeutic strategies.
29471162	6	25	theme	luminal	1060:1066	arg1	concentrations					1073:1086	Boosting luminal H2O2 concentrations	1051:1086	Boosting luminal H2O2 concentrations within a physiological range	1051:1115	Boosting luminal H2O2 concentrations within a physiological range accelerated recovery from colitis, while significantly exceeding this H2O2 level triggered bacteraemia.
29471162	5	26	theme	wild	872:875	arg1	type					877:880	wild type L. johnsonii	872:893	wild type L. johnsonii	872:893	H2O2 generation by wild type L. johnsonii was modified by either deleting or overexpressing the enzymatic H2O2 source(s) followed by orally administering the bacteria before and during DSS colitis.
29471162	3	27	theme	improved	668:675	arg1	strategies					689:698	improved therapeutic strategies	668:698	improved therapeutic strategies	668:698	Although probiotic bacteria have not yet fulfilled fully their promise in clinical trials, understanding the mechanism of how they exert beneficial effects will permit devising improved therapeutic strategies.
29471162	3	28	theme	beneficial	628:637	arg1	effects					639:645	beneficial effects	628:645	beneficial effects	628:645	Although probiotic bacteria have not yet fulfilled fully their promise in clinical trials, understanding the mechanism of how they exert beneficial effects will permit devising improved therapeutic strategies.
29471162	6	29	theme	Boosting	1051:1058	arg1	concentrations					1073:1086	Boosting luminal H2O2 concentrations	1051:1086	Boosting luminal H2O2 concentrations within a physiological range	1051:1115	Boosting luminal H2O2 concentrations within a physiological range accelerated recovery from colitis, while significantly exceeding this H2O2 level triggered bacteraemia.
29471162	3	30	theme	therapeutic	677:687	arg1	strategies					689:698	improved therapeutic strategies	668:698	improved therapeutic strategies	668:698	Although probiotic bacteria have not yet fulfilled fully their promise in clinical trials, understanding the mechanism of how they exert beneficial effects will permit devising improved therapeutic strategies.
29471162	0	31	theme	epithelial	54:63	arg1	restitution					65:75	epithelial restitution	54:75	epithelial restitution	54:75	Hydrogen peroxide production by lactobacilli promotes epithelial restitution during colitis.
29471162	7	32	theme	epithelial	1306:1315	arg1	barrier					1317:1323	the epithelial barrier	1302:1323	the epithelial barrier	1302:1323	This study supports a role for increasing H2O2 within the physiological range at the epithelial barrier, independently of the enzymatic source and/or delivery mechanism, for inducing recovery and remission in IBD.
29471162	1	33	theme	inflammatory	154:165	arg1	disease					112:118	Inflammatory bowel disease	93:118	Inflammatory bowel disease (IBD)	93:124	Inflammatory bowel disease (IBD) is a multifactorial chronic inflammatory disease of the gastrointestinal tract, characterized by cycles of acute flares, recovery and remission phases.
29471162	1	33	theme	inflammatory	154:165	arg1	disease					167:173	a multifactorial chronic inflammatory disease	129:173	a multifactorial chronic inflammatory disease	129:173	Inflammatory bowel disease (IBD) is a multifactorial chronic inflammatory disease of the gastrointestinal tract, characterized by cycles of acute flares, recovery and remission phases.
29471162	4	34	theme	features	738:745	arg1	one					718:720	one	718:720	one	718:720	Here we probe if one of the defining features of lactobacilli, the ability to generate nanomolar H2O2, contributes to their beneficial role in colitis.
29471162	4	34	theme	features	738:745	arg1	ability					768:774	the ability	764:774	the ability to generate nanomolar H2O2	764:801	Here we probe if one of the defining features of lactobacilli, the ability to generate nanomolar H2O2, contributes to their beneficial role in colitis.
29471162	4	34	theme	features	738:745	arg1	features					738:745	the defining features	725:745	the defining features of lactobacilli	725:761	Here we probe if one of the defining features of lactobacilli, the ability to generate nanomolar H2O2, contributes to their beneficial role in colitis.
29471162	5	35	theme	DSS	1038:1040	arg1	colitis					1042:1048	DSS colitis	1038:1048	DSS colitis	1038:1048	H2O2 generation by wild type L. johnsonii was modified by either deleting or overexpressing the enzymatic H2O2 source(s) followed by orally administering the bacteria before and during DSS colitis.
29471162	4	36	dep	ability	768:774	arg1	generate					779:786	generate	779:786	to generate nanomolar H2O2	776:801	Here we probe if one of the defining features of lactobacilli, the ability to generate nanomolar H2O2, contributes to their beneficial role in colitis.
29471162	1	37	theme	acute	233:237	arg1	flares					239:244	acute flares	233:244	acute flares	233:244	Inflammatory bowel disease (IBD) is a multifactorial chronic inflammatory disease of the gastrointestinal tract, characterized by cycles of acute flares, recovery and remission phases.
29471162	3	38	theme	clinical	565:572	arg1	trials					574:579	clinical trials	565:579	clinical trials	565:579	Although probiotic bacteria have not yet fulfilled fully their promise in clinical trials, understanding the mechanism of how they exert beneficial effects will permit devising improved therapeutic strategies.
29471162	5	39	dep	type	877:880	arg1	johnsonii					885:893	wild type L. johnsonii	872:893	wild type L. johnsonii	872:893	H2O2 generation by wild type L. johnsonii was modified by either deleting or overexpressing the enzymatic H2O2 source(s) followed by orally administering the bacteria before and during DSS colitis.
29471162	4	40	from	role	836:839	arg1	colitis					844:850	colitis	844:850	colitis	844:850	Here we probe if one of the defining features of lactobacilli, the ability to generate nanomolar H2O2, contributes to their beneficial role in colitis.
29471162	1	41	theme	multifactorial	131:144	arg1	disease					112:118	Inflammatory bowel disease	93:118	Inflammatory bowel disease (IBD)	93:124	Inflammatory bowel disease (IBD) is a multifactorial chronic inflammatory disease of the gastrointestinal tract, characterized by cycles of acute flares, recovery and remission phases.
29471162	1	41	theme	multifactorial	131:144	arg1	disease					167:173	a multifactorial chronic inflammatory disease	129:173	a multifactorial chronic inflammatory disease	129:173	Inflammatory bowel disease (IBD) is a multifactorial chronic inflammatory disease of the gastrointestinal tract, characterized by cycles of acute flares, recovery and remission phases.
29471162	1	42	theme	flares	239:244	arg1	cycles					223:228	cycles	223:228	cycles of acute flares	223:244	Inflammatory bowel disease (IBD) is a multifactorial chronic inflammatory disease of the gastrointestinal tract, characterized by cycles of acute flares, recovery and remission phases.
29471162	7	43	theme	enzymatic	1347:1355	arg1	source					1357:1362	the enzymatic source	1343:1362	the enzymatic source	1343:1362	This study supports a role for increasing H2O2 within the physiological range at the epithelial barrier, independently of the enzymatic source and/or delivery mechanism, for inducing recovery and remission in IBD.
29471162	1	44	theme	chronic	146:152	arg1	disease					112:118	Inflammatory bowel disease	93:118	Inflammatory bowel disease (IBD)	93:124	Inflammatory bowel disease (IBD) is a multifactorial chronic inflammatory disease of the gastrointestinal tract, characterized by cycles of acute flares, recovery and remission phases.
29471162	1	44	theme	chronic	146:152	arg1	disease					167:173	a multifactorial chronic inflammatory disease	129:173	a multifactorial chronic inflammatory disease	129:173	Inflammatory bowel disease (IBD) is a multifactorial chronic inflammatory disease of the gastrointestinal tract, characterized by cycles of acute flares, recovery and remission phases.
29471162	2	45	theme	intestinal	413:422	arg1	homeostasis					424:434	intestinal homeostasis	413:434	intestinal homeostasis	413:434	Treatments for accelerating tissue restitution and prolonging remission are scarce, but altering the microbiota composition to promote intestinal homeostasis is considered a safe, economic and promising approach.
29471162	4	46	theme	beneficial	825:834	arg1	role					836:839	their beneficial role	819:839	their beneficial role in colitis	819:850	Here we probe if one of the defining features of lactobacilli, the ability to generate nanomolar H2O2, contributes to their beneficial role in colitis.
29471162	6	47	from	colitis	1143:1149	arg1	recovery					1129:1136	recovery	1129:1136	recovery from colitis	1129:1149	Boosting luminal H2O2 concentrations within a physiological range accelerated recovery from colitis, while significantly exceeding this H2O2 level triggered bacteraemia.
29471162	2	48	theme	microbiota	379:388	arg1	composition					390:400	the microbiota composition	375:400	the microbiota composition to promote intestinal homeostasis	375:434	Treatments for accelerating tissue restitution and prolonging remission are scarce, but altering the microbiota composition to promote intestinal homeostasis is considered a safe, economic and promising approach.
29471162	7	49	theme	physiological	1279:1291	arg1	range					1293:1297	the physiological range	1275:1297	the physiological range at the epithelial barrier	1275:1323	This study supports a role for increasing H2O2 within the physiological range at the epithelial barrier, independently of the enzymatic source and/or delivery mechanism, for inducing recovery and remission in IBD.
29471162	0	50	theme	peroxide	9:16	arg1	production					18:27	Hydrogen peroxide production	0:27	Hydrogen peroxide production by lactobacilli	0:43	Hydrogen peroxide production by lactobacilli promotes epithelial restitution during colitis.
29665623	8	0	theme	gas	1144:1146	arg1	spectrometry					1168:1179	gas chromatography mass spectrometry	1144:1179	gas chromatography mass spectrometry	1144:1179	MOS did not affect fecal excretion of cholesterol, but increased fecal BAs as well as butyrate in cecum as determined by gas chromatography mass spectrometry.
29665623	5	1	dep	54	742:743	arg1	to					739:740	to	739:740	to	739:740	MOS substantially decreased atherosclerotic lesions up to 54%, as assessed in the valve area of the aortic root.
29665623	1	2	theme	Mannan	164:169	arg1	MOS					189:191	MOS	189:191	MOS	189:191	SCOPE Mannan oligosaccharides (MOS) have proven effective at improving growth performance, while also reducing hyperlipidemia and inflammation.
29665623	1	2	theme	Mannan	164:169	arg1	oligosaccharides					171:186	SCOPE Mannan oligosaccharides	158:186	SCOPE Mannan oligosaccharides (MOS)	158:192	SCOPE Mannan oligosaccharides (MOS) have proven effective at improving growth performance, while also reducing hyperlipidemia and inflammation.
29665623	8	3	theme	fecal	1042:1046	arg1	excretion					1048:1056	fecal excretion	1042:1056	fecal excretion of cholesterol	1042:1071	MOS did not affect fecal excretion of cholesterol, but increased fecal BAs as well as butyrate in cecum as determined by gas chromatography mass spectrometry.
29665623	0	4	theme	Acid	77:80	arg1	Excretion					82:90	Increase Fecal Bile Acid Excretion	57:90	Increase Fecal Bile Acid Excretion	57:90	Dietary Mannan Oligosaccharides Modulate Gut Microbiota, Increase Fecal Bile Acid Excretion, and Decrease Plasma Cholesterol and Atherosclerosis Development.
29665623	9	5	theme	atherosclerosis	1220:1234	arg1	development					1236:1246	atherosclerosis development	1220:1246	atherosclerosis development	1220:1246	CONCLUSION MOS decreased the onset of atherosclerosis development via lowering of plasma cholesterol levels.
29665623	7	6	theme	microbiota	893:902	arg1	composition					904:914	Gut microbiota composition	889:914	Gut microbiota composition	889:914	Gut microbiota composition was determined using 16S rRNA gene sequencing and MOS increased the abundance of cecal Bacteroides ovatus.
29665623	3	7	theme	well-established	510:525	arg1	mice					502:505	CETP (E3L.CETP) mice	486:505	CETP (E3L.CETP) mice	486:505	CETP (E3L.CETP) mice, a well-established model for human-like lipoprotein metabolism.
29665623	3	7	theme	well-established	510:525	arg1	model					527:531	a well-established model	508:531	a well-established model for human-like lipoprotein metabolism	508:569	CETP (E3L.CETP) mice, a well-established model for human-like lipoprotein metabolism.
29665623	4	8	theme	E3L.CETP	599:606	arg1	mice					608:611	METHODS AND RESULTS Female E3L.CETP mice	572:611	METHODS AND RESULTS Female E3L.CETP mice	572:611	METHODS AND RESULTS Female E3L.CETP mice were fed a high-cholesterol diet, with or without 1% MOS for 14 weeks.
29665623	9	9	theme	development	1236:1246	arg1	onset					1211:1215	the onset	1207:1215	the onset of atherosclerosis development	1207:1246	CONCLUSION MOS decreased the onset of atherosclerosis development via lowering of plasma cholesterol levels.
29665623	10	10	theme	increased	1325:1333	arg1	butyrate					1341:1348	increased cecal butyrate	1325:1348	increased cecal butyrate	1325:1348	These effects were accompanied by increased cecal butyrate and fecal excretion of BAs, presumably mediated via interactions of MOS with the gut microbiota.
29665623	8	11	theme	fecal	1088:1092	arg1	BAs					1094:1096	fecal BAs	1088:1096	fecal BAs as well as butyrate	1088:1116	MOS did not affect fecal excretion of cholesterol, but increased fecal BAs as well as butyrate in cecum as determined by gas chromatography mass spectrometry.
29665623	5	12	theme	root	791:794	arg1	area					772:775	the valve area	762:775	the valve area of the aortic root	762:794	MOS substantially decreased atherosclerotic lesions up to 54%, as assessed in the valve area of the aortic root.
29665623	10	13	theme	cecal	1335:1339	arg1	butyrate					1341:1348	increased cecal butyrate	1325:1348	increased cecal butyrate	1325:1348	These effects were accompanied by increased cecal butyrate and fecal excretion of BAs, presumably mediated via interactions of MOS with the gut microbiota.
29665623	0	14	dep	Decrease	97:104	arg1	Cholesterol					113:123	Plasma Cholesterol	106:123	Plasma Cholesterol	106:123	Dietary Mannan Oligosaccharides Modulate Gut Microbiota, Increase Fecal Bile Acid Excretion, and Decrease Plasma Cholesterol and Atherosclerosis Development.
29665623	8	15	theme	cholesterol	1061:1071	arg1	excretion					1048:1056	fecal excretion	1042:1056	fecal excretion of cholesterol	1042:1071	MOS did not affect fecal excretion of cholesterol, but increased fecal BAs as well as butyrate in cecum as determined by gas chromatography mass spectrometry.
29665623	0	16	theme	Plasma	106:111	arg1	Cholesterol					113:123	Plasma Cholesterol	106:123	Plasma Cholesterol	106:123	Dietary Mannan Oligosaccharides Modulate Gut Microbiota, Increase Fecal Bile Acid Excretion, and Decrease Plasma Cholesterol and Atherosclerosis Development.
29665623	6	17	theme	bile	846:849	arg1	BAs					858:860	BAs	858:860	BAs	858:860	In blood, IL-1RA, monocyte subtypes, lipids, and bile acids (BAs) were not affected by MOS.
29665623	6	17	theme	bile	846:849	arg1	acids					851:855	bile acids	846:855	bile acids (BAs)	846:861	In blood, IL-1RA, monocyte subtypes, lipids, and bile acids (BAs) were not affected by MOS.
29665623	5	18	theme	aortic	784:789	arg1	root					791:794	the aortic root	780:794	the aortic root	780:794	MOS substantially decreased atherosclerotic lesions up to 54%, as assessed in the valve area of the aortic root.
29665623	3	19	theme	human-like	537:546	arg1	metabolism					560:569	human-like lipoprotein metabolism	537:569	human-like lipoprotein metabolism	537:569	CETP (E3L.CETP) mice, a well-established model for human-like lipoprotein metabolism.
29665623	2	20	theme	dietary	411:417	arg1	MOS					419:421	dietary MOS	411:421	dietary MOS	411:421	As atherosclerosis is accelerated both by hyperlipidemia and inflammation, we aim to determine the effect of dietary MOS on atherosclerosis development in hyperlipidemic ApoE*3-Leiden.
29665623	10	21	theme	MOS	1418:1420	arg1	interactions					1402:1413	interactions	1402:1413	interactions of MOS with the gut microbiota	1402:1444	These effects were accompanied by increased cecal butyrate and fecal excretion of BAs, presumably mediated via interactions of MOS with the gut microbiota.
29665623	7	22	theme	ovatus	1015:1020	arg1	abundance					984:992	the abundance	980:992	the abundance of cecal Bacteroides ovatus	980:1020	Gut microbiota composition was determined using 16S rRNA gene sequencing and MOS increased the abundance of cecal Bacteroides ovatus.
29665623	0	23	theme	Dietary	0:6	arg1	Oligosaccharides					15:30	Dietary Mannan Oligosaccharides	0:30	Dietary Mannan Oligosaccharides	0:30	Dietary Mannan Oligosaccharides Modulate Gut Microbiota, Increase Fecal Bile Acid Excretion, and Decrease Plasma Cholesterol and Atherosclerosis Development.
29665623	4	24	theme	%	664:664	arg1	MOS					666:668	1% MOS	663:668	1% MOS for 14 weeks	663:681	METHODS AND RESULTS Female E3L.CETP mice were fed a high-cholesterol diet, with or without 1% MOS for 14 weeks.
29665623	0	25	theme	Mannan	8:13	arg1	Oligosaccharides					15:30	Dietary Mannan Oligosaccharides	0:30	Dietary Mannan Oligosaccharides	0:30	Dietary Mannan Oligosaccharides Modulate Gut Microbiota, Increase Fecal Bile Acid Excretion, and Decrease Plasma Cholesterol and Atherosclerosis Development.
29665623	9	26	theme	plasma	1264:1269	arg1	levels					1283:1288	plasma cholesterol levels	1264:1288	plasma cholesterol levels	1264:1288	CONCLUSION MOS decreased the onset of atherosclerosis development via lowering of plasma cholesterol levels.
29665623	5	27	dep	lesions	728:734	arg1	%					744:744	%	744:744	%	744:744	MOS substantially decreased atherosclerotic lesions up to 54%, as assessed in the valve area of the aortic root.
29665623	10	28	dep	accompanied	1310:1320	arg1	mediated					1389:1396	mediated	1389:1396	mediated via interactions of MOS with the gut microbiota	1389:1444	These effects were accompanied by increased cecal butyrate and fecal excretion of BAs, presumably mediated via interactions of MOS with the gut microbiota.
29665623	2	29	theme	ApoE	472:475	arg1	*					476:476	hyperlipidemic ApoE*3-Leiden	457:484	hyperlipidemic ApoE*3-Leiden	457:484	As atherosclerosis is accelerated both by hyperlipidemia and inflammation, we aim to determine the effect of dietary MOS on atherosclerosis development in hyperlipidemic ApoE*3-Leiden.
29665623	9	30	theme	cholesterol	1271:1281	arg1	levels					1283:1288	plasma cholesterol levels	1264:1288	plasma cholesterol levels	1264:1288	CONCLUSION MOS decreased the onset of atherosclerosis development via lowering of plasma cholesterol levels.
29665623	8	31	theme	mass	1163:1166	arg1	spectrometry					1168:1179	gas chromatography mass spectrometry	1144:1179	gas chromatography mass spectrometry	1144:1179	MOS did not affect fecal excretion of cholesterol, but increased fecal BAs as well as butyrate in cecum as determined by gas chromatography mass spectrometry.
29665623	2	32	dep	hyperlipidemia	344:357	arg1	both					336:339	both	336:339	both	336:339	As atherosclerosis is accelerated both by hyperlipidemia and inflammation, we aim to determine the effect of dietary MOS on atherosclerosis development in hyperlipidemic ApoE*3-Leiden.
29665623	2	33	theme	hyperlipidemic	457:470	arg1	*					476:476	hyperlipidemic ApoE*3-Leiden	457:484	hyperlipidemic ApoE*3-Leiden	457:484	As atherosclerosis is accelerated both by hyperlipidemia and inflammation, we aim to determine the effect of dietary MOS on atherosclerosis development in hyperlipidemic ApoE*3-Leiden.
29665623	4	34	theme	METHODS	572:578	arg1	mice					608:611	METHODS AND RESULTS Female E3L.CETP mice	572:611	METHODS AND RESULTS Female E3L.CETP mice	572:611	METHODS AND RESULTS Female E3L.CETP mice were fed a high-cholesterol diet, with or without 1% MOS for 14 weeks.
29665623	7	35	theme	Bacteroides	1003:1013	arg1	ovatus					1015:1020	cecal Bacteroides ovatus	997:1020	cecal Bacteroides ovatus	997:1020	Gut microbiota composition was determined using 16S rRNA gene sequencing and MOS increased the abundance of cecal Bacteroides ovatus.
29665623	7	36	theme	Gut	889:891	arg1	composition					904:914	Gut microbiota composition	889:914	Gut microbiota composition	889:914	Gut microbiota composition was determined using 16S rRNA gene sequencing and MOS increased the abundance of cecal Bacteroides ovatus.
29665623	10	37	with	interactions	1402:1413	arg1	microbiota					1435:1444	the gut microbiota	1427:1444	the gut microbiota	1427:1444	These effects were accompanied by increased cecal butyrate and fecal excretion of BAs, presumably mediated via interactions of MOS with the gut microbiota.
29665623	3	38	theme	CETP	486:489	arg1	mice					502:505	CETP (E3L.CETP) mice	486:505	CETP (E3L.CETP) mice	486:505	CETP (E3L.CETP) mice, a well-established model for human-like lipoprotein metabolism.
29665623	3	38	theme	CETP	486:489	arg1	model					527:531	a well-established model	508:531	a well-established model for human-like lipoprotein metabolism	508:569	CETP (E3L.CETP) mice, a well-established model for human-like lipoprotein metabolism.
29665623	4	39	theme	Female	592:597	arg1	mice					608:611	METHODS AND RESULTS Female E3L.CETP mice	572:611	METHODS AND RESULTS Female E3L.CETP mice	572:611	METHODS AND RESULTS Female E3L.CETP mice were fed a high-cholesterol diet, with or without 1% MOS for 14 weeks.
29665623	4	40	theme	high-cholesterol	624:639	arg1	diet					641:644	a high-cholesterol diet	622:644	a high-cholesterol diet	622:644	METHODS AND RESULTS Female E3L.CETP mice were fed a high-cholesterol diet, with or without 1% MOS for 14 weeks.
29665623	2	41	theme	atherosclerosis	426:440	arg1	development					442:452	atherosclerosis development	426:452	atherosclerosis development in hyperlipidemic ApoE*3-Leiden	426:484	As atherosclerosis is accelerated both by hyperlipidemia and inflammation, we aim to determine the effect of dietary MOS on atherosclerosis development in hyperlipidemic ApoE*3-Leiden.
29665623	5	42	theme	valve	766:770	arg1	area					772:775	the valve area	762:775	the valve area of the aortic root	762:794	MOS substantially decreased atherosclerotic lesions up to 54%, as assessed in the valve area of the aortic root.
29665623	4	43	theme	RESULTS	584:590	arg1	mice					608:611	METHODS AND RESULTS Female E3L.CETP mice	572:611	METHODS AND RESULTS Female E3L.CETP mice	572:611	METHODS AND RESULTS Female E3L.CETP mice were fed a high-cholesterol diet, with or without 1% MOS for 14 weeks.
29665623	10	44	theme	gut	1431:1433	arg1	microbiota					1435:1444	the gut microbiota	1427:1444	the gut microbiota	1427:1444	These effects were accompanied by increased cecal butyrate and fecal excretion of BAs, presumably mediated via interactions of MOS with the gut microbiota.
29665623	4	45	theme	1	663:663	arg1	%					664:664	%	664:664	%	664:664	METHODS AND RESULTS Female E3L.CETP mice were fed a high-cholesterol diet, with or without 1% MOS for 14 weeks.
29665623	0	46	theme	Gut	41:43	arg1	Microbiota					45:54	Gut Microbiota	41:54	Gut Microbiota	41:54	Dietary Mannan Oligosaccharides Modulate Gut Microbiota, Increase Fecal Bile Acid Excretion, and Decrease Plasma Cholesterol and Atherosclerosis Development.
29665623	0	47	theme	Atherosclerosis	129:143	arg1	Development					145:155	Atherosclerosis Development	129:155	Atherosclerosis Development	129:155	Dietary Mannan Oligosaccharides Modulate Gut Microbiota, Increase Fecal Bile Acid Excretion, and Decrease Plasma Cholesterol and Atherosclerosis Development.
29665623	9	48	theme	levels	1283:1288	arg1	lowering					1252:1259	lowering	1252:1259	lowering of plasma cholesterol levels	1252:1288	CONCLUSION MOS decreased the onset of atherosclerosis development via lowering of plasma cholesterol levels.
29665623	2	49	from	effect	401:406	arg1	development					442:452	atherosclerosis development	426:452	atherosclerosis development in hyperlipidemic ApoE*3-Leiden	426:484	As atherosclerosis is accelerated both by hyperlipidemia and inflammation, we aim to determine the effect of dietary MOS on atherosclerosis development in hyperlipidemic ApoE*3-Leiden.
29665623	2	50	theme	MOS	419:421	arg1	effect					401:406	the effect	397:406	the effect of dietary MOS on atherosclerosis development in hyperlipidemic ApoE*3-Leiden	397:484	As atherosclerosis is accelerated both by hyperlipidemia and inflammation, we aim to determine the effect of dietary MOS on atherosclerosis development in hyperlipidemic ApoE*3-Leiden.
29665623	0	51	theme	Increase	57:64	arg1	Excretion					82:90	Increase Fecal Bile Acid Excretion	57:90	Increase Fecal Bile Acid Excretion	57:90	Dietary Mannan Oligosaccharides Modulate Gut Microbiota, Increase Fecal Bile Acid Excretion, and Decrease Plasma Cholesterol and Atherosclerosis Development.
29665623	5	52	dep	%	744:744	arg1	54					742:743	54	742:743	54	742:743	MOS substantially decreased atherosclerotic lesions up to 54%, as assessed in the valve area of the aortic root.
29665623	6	53	theme	monocyte	815:822	arg1	subtypes					824:831	monocyte subtypes	815:831	monocyte subtypes	815:831	In blood, IL-1RA, monocyte subtypes, lipids, and bile acids (BAs) were not affected by MOS.
29665623	7	54	theme	cecal	997:1001	arg1	ovatus					1015:1020	cecal Bacteroides ovatus	997:1020	cecal Bacteroides ovatus	997:1020	Gut microbiota composition was determined using 16S rRNA gene sequencing and MOS increased the abundance of cecal Bacteroides ovatus.
29665623	9	55	theme	CONCLUSION	1182:1191	arg1	MOS					1193:1195	CONCLUSION MOS	1182:1195	CONCLUSION MOS	1182:1195	CONCLUSION MOS decreased the onset of atherosclerosis development via lowering of plasma cholesterol levels.
29665623	7	56	theme	rRNA	941:944	arg1	sequencing					951:960	16S rRNA gene sequencing	937:960	16S rRNA gene sequencing	937:960	Gut microbiota composition was determined using 16S rRNA gene sequencing and MOS increased the abundance of cecal Bacteroides ovatus.
29665623	10	57	theme	fecal	1354:1358	arg1	excretion					1360:1368	fecal excretion	1354:1368	fecal excretion of BAs	1354:1375	These effects were accompanied by increased cecal butyrate and fecal excretion of BAs, presumably mediated via interactions of MOS with the gut microbiota.
29665623	10	58	theme	BAs	1373:1375	arg1	butyrate					1341:1348	increased cecal butyrate	1325:1348	increased cecal butyrate	1325:1348	These effects were accompanied by increased cecal butyrate and fecal excretion of BAs, presumably mediated via interactions of MOS with the gut microbiota.
29665623	10	58	theme	BAs	1373:1375	arg1	excretion					1360:1368	fecal excretion	1354:1368	fecal excretion of BAs	1354:1375	These effects were accompanied by increased cecal butyrate and fecal excretion of BAs, presumably mediated via interactions of MOS with the gut microbiota.
29665623	7	59	theme	gene	946:949	arg1	sequencing					951:960	16S rRNA gene sequencing	937:960	16S rRNA gene sequencing	937:960	Gut microbiota composition was determined using 16S rRNA gene sequencing and MOS increased the abundance of cecal Bacteroides ovatus.
29665623	1	60	theme	growth	229:234	arg1	performance					236:246	growth performance	229:246	growth performance	229:246	SCOPE Mannan oligosaccharides (MOS) have proven effective at improving growth performance, while also reducing hyperlipidemia and inflammation.
29665623	3	61	theme	lipoprotein	548:558	arg1	metabolism					560:569	human-like lipoprotein metabolism	537:569	human-like lipoprotein metabolism	537:569	CETP (E3L.CETP) mice, a well-established model for human-like lipoprotein metabolism.
29665623	5	62	theme	atherosclerotic	712:726	arg1	lesions					728:734	atherosclerotic lesions	712:734	atherosclerotic lesions up to 54%	712:744	MOS substantially decreased atherosclerotic lesions up to 54%, as assessed in the valve area of the aortic root.
29665623	0	63	theme	Bile	72:75	arg1	Excretion					82:90	Increase Fecal Bile Acid Excretion	57:90	Increase Fecal Bile Acid Excretion	57:90	Dietary Mannan Oligosaccharides Modulate Gut Microbiota, Increase Fecal Bile Acid Excretion, and Decrease Plasma Cholesterol and Atherosclerosis Development.
29665623	7	64	theme	16S	937:939	arg1	sequencing					951:960	16S rRNA gene sequencing	937:960	16S rRNA gene sequencing	937:960	Gut microbiota composition was determined using 16S rRNA gene sequencing and MOS increased the abundance of cecal Bacteroides ovatus.
29665623	8	65	theme	chromatography	1148:1161	arg1	spectrometry					1168:1179	gas chromatography mass spectrometry	1144:1179	gas chromatography mass spectrometry	1144:1179	MOS did not affect fecal excretion of cholesterol, but increased fecal BAs as well as butyrate in cecum as determined by gas chromatography mass spectrometry.
29665623	0	66	theme	Fecal	66:70	arg1	Excretion					82:90	Increase Fecal Bile Acid Excretion	57:90	Increase Fecal Bile Acid Excretion	57:90	Dietary Mannan Oligosaccharides Modulate Gut Microbiota, Increase Fecal Bile Acid Excretion, and Decrease Plasma Cholesterol and Atherosclerosis Development.
29665623	2	67	from	development	442:452	arg1	*					476:476	hyperlipidemic ApoE*3-Leiden	457:484	hyperlipidemic ApoE*3-Leiden	457:484	As atherosclerosis is accelerated both by hyperlipidemia and inflammation, we aim to determine the effect of dietary MOS on atherosclerosis development in hyperlipidemic ApoE*3-Leiden.
29665623	1	68	theme	SCOPE	158:162	arg1	MOS					189:191	MOS	189:191	MOS	189:191	SCOPE Mannan oligosaccharides (MOS) have proven effective at improving growth performance, while also reducing hyperlipidemia and inflammation.
29665623	1	68	theme	SCOPE	158:162	arg1	oligosaccharides					171:186	SCOPE Mannan oligosaccharides	158:186	SCOPE Mannan oligosaccharides (MOS)	158:192	SCOPE Mannan oligosaccharides (MOS) have proven effective at improving growth performance, while also reducing hyperlipidemia and inflammation.
30706742	6	0	theme	mice	821:824	arg1	Half					806:809	Half	806:809	Half of the GF mice	806:824	Half of the GF mice were colonized with BF for 28 days before CRC induction by AOM/DSS.
30706742	9	1	theme	significant	1066:1076	arg1	decrease					1078:1085	a significant decrease	1064:1085	a significant decrease in tumor number and tumor incidence in the BF/AOM/DSS group	1064:1145	Interestingly, there was a significant decrease in tumor number and tumor incidence in the BF/AOM/DSS group as compared to the GF/AOM/DSS group.
30706742	2	2	theme	microflora	370:379	arg1	composition					351:361	the composition	347:361	the composition of gut microflora	347:379	Previous studies have indicated that the composition of gut microflora may be involved in CAC induction and progress.
30706742	1	3	theme	colorectal	285:294	arg1	CAC					304:306	CAC	304:306	CAC	304:306	Objective: Inflammatory bowel disease (IBD) is generally considered as a major risk factor in the progression of colitis-associated colorectal cancer (CAC).
30706742	1	3	theme	colorectal	285:294	arg1	cancer					296:301	colitis-associated colorectal cancer	266:301	colitis-associated colorectal cancer (CAC)	266:307	Objective: Inflammatory bowel disease (IBD) is generally considered as a major risk factor in the progression of colitis-associated colorectal cancer (CAC).
30706742	11	4	theme	decreased	1336:1344	arg1	numbers					1346:1352	decreased numbers	1336:1352	decreased numbers of cell proliferation (PCNA) and inflammatory cell (granulocytes) infiltration	1336:1431	In addition, immunohistochemistry staining found decreased numbers of cell proliferation (PCNA) and inflammatory cell (granulocytes) infiltration in the colon mucosa of the BF group.
30706742	11	5	theme	colon	1440:1444	arg1	mucosa					1446:1451	the colon mucosa	1436:1451	the colon mucosa of the BF group	1436:1467	In addition, immunohistochemistry staining found decreased numbers of cell proliferation (PCNA) and inflammatory cell (granulocytes) infiltration in the colon mucosa of the BF group.
30706742	13	6	theme	cell	1681:1684	arg1	molecules					1708:1716	cell proliferation-related molecules	1681:1716	cell proliferation-related molecules	1681:1716	Taking together, the BF colonization significantly ameliorated AOM/DSS-induced CRC by suppressing the activity of cell proliferation-related molecules and reducing the number of inflammatory cells.
30706742	9	7	theme	tumor	1107:1111	arg1	incidence					1113:1121	tumor incidence	1107:1121	tumor incidence	1107:1121	Interestingly, there was a significant decrease in tumor number and tumor incidence in the BF/AOM/DSS group as compared to the GF/AOM/DSS group.
30706742	5	8	theme	BF	796:797	arg1	group					799:803	BF group	796:803	BF group	796:803	Materials and methods: Total 22 GF mice were divided into two groups: GF and BF group.
30706742	5	8	theme	BF	796:797	arg1	groups					781:786	two groups	777:786	two groups: GF and BF group	777:803	Materials and methods: Total 22 GF mice were divided into two groups: GF and BF group.
30706742	6	9	theme	CRC	868:870	arg1	induction					872:880	CRC induction	868:880	CRC induction by AOM/DSS	868:891	Half of the GF mice were colonized with BF for 28 days before CRC induction by AOM/DSS.
30706742	13	10	theme	molecules	1708:1716	arg1	activity					1669:1676	the activity	1665:1676	the activity of cell proliferation-related molecules	1665:1716	Taking together, the BF colonization significantly ameliorated AOM/DSS-induced CRC by suppressing the activity of cell proliferation-related molecules and reducing the number of inflammatory cells.
30706742	1	11	theme	major	226:230	arg1	disease					183:189	Inflammatory bowel disease	164:189	Inflammatory bowel disease (IBD)	164:195	Objective: Inflammatory bowel disease (IBD) is generally considered as a major risk factor in the progression of colitis-associated colorectal cancer (CAC).
30706742	1	11	theme	major	226:230	arg1	factor					237:242	a major risk factor	224:242	a major risk factor in the progression of colitis-associated colorectal cancer (CAC)	224:307	Objective: Inflammatory bowel disease (IBD) is generally considered as a major risk factor in the progression of colitis-associated colorectal cancer (CAC).
30706742	9	12	theme	BF/AOM/DSS	1130:1139	arg1	group					1141:1145	the BF/AOM/DSS group	1126:1145	the BF/AOM/DSS group	1126:1145	Interestingly, there was a significant decrease in tumor number and tumor incidence in the BF/AOM/DSS group as compared to the GF/AOM/DSS group.
30706742	12	13	theme	β-catenin	1474:1482	arg1	staining					1484:1491	The β-catenin staining	1470:1491	The β-catenin staining in the BF/AOM/DSS group	1470:1515	The β-catenin staining in the BF/AOM/DSS group had fewer and weaker positive signal expressions.
30706742	11	14	theme	granulocytes	1406:1417	arg1	infiltration					1420:1431	inflammatory cell (granulocytes) infiltration	1387:1431	inflammatory cell (granulocytes) infiltration	1387:1431	In addition, immunohistochemistry staining found decreased numbers of cell proliferation (PCNA) and inflammatory cell (granulocytes) infiltration in the colon mucosa of the BF group.
30706742	1	15	theme	bowel	177:181	arg1	IBD					192:194	IBD	192:194	IBD	192:194	Objective: Inflammatory bowel disease (IBD) is generally considered as a major risk factor in the progression of colitis-associated colorectal cancer (CAC).
30706742	1	15	theme	bowel	177:181	arg1	disease					183:189	Inflammatory bowel disease	164:189	Inflammatory bowel disease (IBD)	164:195	Objective: Inflammatory bowel disease (IBD) is generally considered as a major risk factor in the progression of colitis-associated colorectal cancer (CAC).
30706742	1	15	theme	bowel	177:181	arg1	factor					237:242	a major risk factor	224:242	a major risk factor in the progression of colitis-associated colorectal cancer (CAC)	224:307	Objective: Inflammatory bowel disease (IBD) is generally considered as a major risk factor in the progression of colitis-associated colorectal cancer (CAC).
30706742	11	16	theme	cell	1400:1403	arg1	infiltration					1420:1431	inflammatory cell (granulocytes) infiltration	1387:1431	inflammatory cell (granulocytes) infiltration	1387:1431	In addition, immunohistochemistry staining found decreased numbers of cell proliferation (PCNA) and inflammatory cell (granulocytes) infiltration in the colon mucosa of the BF group.
30706742	0	17	theme	azoxymethane/dextran	71:90	arg1	sodium					100:105	azoxymethane/dextran sulfate sodium	71:105	azoxymethane/dextran sulfate sodium induced colitis-associated colorectal cancer	71:150	The germ-free mice monocolonization with Bacteroides fragilis improves azoxymethane/dextran sulfate sodium induced colitis-associated colorectal cancer.
30706742	9	18	theme	GF/AOM/DSS	1166:1175	arg1	group					1177:1181	the GF/AOM/DSS group	1162:1181	the GF/AOM/DSS group	1162:1181	Interestingly, there was a significant decrease in tumor number and tumor incidence in the BF/AOM/DSS group as compared to the GF/AOM/DSS group.
30706742	14	19	theme	Symbiotic	1778:1786	arg1	BF					1788:1789	Symbiotic BF	1778:1789	Symbiotic BF	1778:1789	Conclusions: Symbiotic BF may play a pivotal role in maintaining the gastrointestinal immunophysiologic balance and regulating anti-tumorigenesis responses.
30706742	4	20	theme	sulfate	674:680	arg1	DSS					690:692	dextran sulfate sodium (DSS)	666:693	dextran sulfate sodium (DSS)	666:693	This study was aimed to investigate the protective role of BF in a colorectal cancer (CRC) model induced by azoxymethane (AOM) and dextran sulfate sodium (DSS) in germ-free (GF) mice.
30706742	13	21	theme	AOM/DSS-induced	1630:1644	arg1	CRC					1646:1648	AOM/DSS-induced CRC	1630:1648	AOM/DSS-induced CRC	1630:1648	Taking together, the BF colonization significantly ameliorated AOM/DSS-induced CRC by suppressing the activity of cell proliferation-related molecules and reducing the number of inflammatory cells.
30706742	11	22	theme	infiltration	1420:1431	arg1	numbers					1346:1352	decreased numbers	1336:1352	decreased numbers of cell proliferation (PCNA) and inflammatory cell (granulocytes) infiltration	1336:1431	In addition, immunohistochemistry staining found decreased numbers of cell proliferation (PCNA) and inflammatory cell (granulocytes) infiltration in the colon mucosa of the BF group.
30706742	0	23	theme	colitis-associated	115:132	arg1	cancer					145:150	colitis-associated colorectal cancer	115:150	colitis-associated colorectal cancer	115:150	The germ-free mice monocolonization with Bacteroides fragilis improves azoxymethane/dextran sulfate sodium induced colitis-associated colorectal cancer.
30706742	11	24	theme	proliferation	1362:1374	arg1	numbers					1346:1352	decreased numbers	1336:1352	decreased numbers of cell proliferation (PCNA) and inflammatory cell (granulocytes) infiltration	1336:1431	In addition, immunohistochemistry staining found decreased numbers of cell proliferation (PCNA) and inflammatory cell (granulocytes) infiltration in the colon mucosa of the BF group.
30706742	4	25	from	role	586:589	arg1	model					626:630	a colorectal cancer (CRC) model	600:630	a colorectal cancer (CRC) model induced by azoxymethane (AOM) and dextran sulfate sodium (DSS) in germ-free (GF) mice	600:716	This study was aimed to investigate the protective role of BF in a colorectal cancer (CRC) model induced by azoxymethane (AOM) and dextran sulfate sodium (DSS) in germ-free (GF) mice.
30706742	13	26	theme	BF	1588:1589	arg1	colonization					1591:1602	the BF colonization	1584:1602	the BF colonization	1584:1602	Taking together, the BF colonization significantly ameliorated AOM/DSS-induced CRC by suppressing the activity of cell proliferation-related molecules and reducing the number of inflammatory cells.
30706742	4	27	theme	germ-free	698:706	arg1	mice					713:716	germ-free (GF) mice	698:716	germ-free (GF) mice	698:716	This study was aimed to investigate the protective role of BF in a colorectal cancer (CRC) model induced by azoxymethane (AOM) and dextran sulfate sodium (DSS) in germ-free (GF) mice.
30706742	7	28	dep	increased	919:927	arg1	%					949:949	100%	946:949	100%	946:949	Results: BF colonization increased animal survival (100%).
30706742	2	29	theme	CAC	400:402	arg1	induction					404:412	CAC induction	400:412	CAC induction	400:412	Previous studies have indicated that the composition of gut microflora may be involved in CAC induction and progress.
30706742	5	30	theme	GF	751:752	arg1	mice					754:757	Total 22 GF mice	742:757	Total 22 GF mice	742:757	Materials and methods: Total 22 GF mice were divided into two groups: GF and BF group.
30706742	12	31	theme	weaker	1531:1536	arg1	expressions					1554:1564	fewer and weaker positive signal expressions	1521:1564	fewer and weaker positive signal expressions	1521:1564	The β-catenin staining in the BF/AOM/DSS group had fewer and weaker positive signal expressions.
30706742	4	32	theme	GF	709:710	arg1	mice					713:716	germ-free (GF) mice	698:716	germ-free (GF) mice	698:716	This study was aimed to investigate the protective role of BF in a colorectal cancer (CRC) model induced by azoxymethane (AOM) and dextran sulfate sodium (DSS) in germ-free (GF) mice.
30706742	0	33	theme	mice	14:17	arg1	monocolonization					19:34	The germ-free mice monocolonization	0:34	The germ-free mice monocolonization with Bacteroides fragilis	0:60	The germ-free mice monocolonization with Bacteroides fragilis improves azoxymethane/dextran sulfate sodium induced colitis-associated colorectal cancer.
30706742	12	34	theme	signal	1547:1552	arg1	expressions					1554:1564	fewer and weaker positive signal expressions	1521:1564	fewer and weaker positive signal expressions	1521:1564	The β-catenin staining in the BF/AOM/DSS group had fewer and weaker positive signal expressions.
30706742	3	35	theme	Bacteroides	428:438	arg1	anaerobe					473:480	a Gram-negative anaerobe	457:480	a Gram-negative anaerobe belonging to colonic symbiotic bacteria of the host	457:532	Bacteroides fragilis (BF) is a Gram-negative anaerobe belonging to colonic symbiotic bacteria of the host.
30706742	3	35	theme	Bacteroides	428:438	arg1	BF					450:451	BF	450:451	BF	450:451	Bacteroides fragilis (BF) is a Gram-negative anaerobe belonging to colonic symbiotic bacteria of the host.
30706742	3	35	theme	Bacteroides	428:438	arg1	fragilis					440:447	Bacteroides fragilis	428:447	Bacteroides fragilis (BF)	428:452	Bacteroides fragilis (BF) is a Gram-negative anaerobe belonging to colonic symbiotic bacteria of the host.
30706742	10	36	theme	adenocarcinoma/adenoma	1188:1209	arg1	incidence					1211:1219	The adenocarcinoma/adenoma incidence	1184:1219	The adenocarcinoma/adenoma incidence	1184:1219	The adenocarcinoma/adenoma incidence and histologic score were also decreased in the BF/AOM/DSS group.
30706742	8	37	theme	cecum/body	970:979	arg1	ratio					988:992	cecum/body weight ratio	970:992	cecum/body weight ratio	970:992	Cecum weight and cecum/body weight ratio significantly decreased in BF/AOM/DSS group.
30706742	3	38	theme	symbiotic	503:511	arg1	bacteria					513:520	colonic symbiotic bacteria	495:520	colonic symbiotic bacteria of the host	495:532	Bacteroides fragilis (BF) is a Gram-negative anaerobe belonging to colonic symbiotic bacteria of the host.
30706742	5	39	theme	Total	742:746	arg1	mice					754:757	Total 22 GF mice	742:757	Total 22 GF mice	742:757	Materials and methods: Total 22 GF mice were divided into two groups: GF and BF group.
30706742	10	40	theme	BF/AOM/DSS	1269:1278	arg1	group					1280:1284	the BF/AOM/DSS group	1265:1284	the BF/AOM/DSS group	1265:1284	The adenocarcinoma/adenoma incidence and histologic score were also decreased in the BF/AOM/DSS group.
30706742	3	41	theme	host	529:532	arg1	bacteria					513:520	colonic symbiotic bacteria	495:520	colonic symbiotic bacteria of the host	495:532	Bacteroides fragilis (BF) is a Gram-negative anaerobe belonging to colonic symbiotic bacteria of the host.
30706742	8	42	theme	BF/AOM/DSS	1021:1030	arg1	group					1032:1036	BF/AOM/DSS group	1021:1036	BF/AOM/DSS group	1021:1036	Cecum weight and cecum/body weight ratio significantly decreased in BF/AOM/DSS group.
30706742	4	43	theme	cancer	613:618	arg1	model					626:630	a colorectal cancer (CRC) model	600:630	a colorectal cancer (CRC) model induced by azoxymethane (AOM) and dextran sulfate sodium (DSS) in germ-free (GF) mice	600:716	This study was aimed to investigate the protective role of BF in a colorectal cancer (CRC) model induced by azoxymethane (AOM) and dextran sulfate sodium (DSS) in germ-free (GF) mice.
30706742	13	44	theme	cells	1758:1762	arg1	number					1735:1740	the number	1731:1740	the number of inflammatory cells	1731:1762	Taking together, the BF colonization significantly ameliorated AOM/DSS-induced CRC by suppressing the activity of cell proliferation-related molecules and reducing the number of inflammatory cells.
30706742	1	45	theme	colitis-associated	266:283	arg1	CAC					304:306	CAC	304:306	CAC	304:306	Objective: Inflammatory bowel disease (IBD) is generally considered as a major risk factor in the progression of colitis-associated colorectal cancer (CAC).
30706742	1	45	theme	colitis-associated	266:283	arg1	cancer					296:301	colitis-associated colorectal cancer	266:301	colitis-associated colorectal cancer (CAC)	266:307	Objective: Inflammatory bowel disease (IBD) is generally considered as a major risk factor in the progression of colitis-associated colorectal cancer (CAC).
30706742	6	46	theme	GF	818:819	arg1	mice					821:824	the GF mice	814:824	the GF mice	814:824	Half of the GF mice were colonized with BF for 28 days before CRC induction by AOM/DSS.
30706742	2	47	theme	Previous	310:317	arg1	studies					319:325	Previous studies	310:325	Previous studies	310:325	Previous studies have indicated that the composition of gut microflora may be involved in CAC induction and progress.
30706742	1	48	theme	cancer	296:301	arg1	progression					251:261	the progression	247:261	the progression of colitis-associated colorectal cancer (CAC)	247:307	Objective: Inflammatory bowel disease (IBD) is generally considered as a major risk factor in the progression of colitis-associated colorectal cancer (CAC).
30706742	4	49	theme	BF	594:595	arg1	role					586:589	the protective role	571:589	the protective role of BF in a colorectal cancer (CRC) model induced by azoxymethane (AOM) and dextran sulfate sodium (DSS) in germ-free (GF) mice	571:716	This study was aimed to investigate the protective role of BF in a colorectal cancer (CRC) model induced by azoxymethane (AOM) and dextran sulfate sodium (DSS) in germ-free (GF) mice.
30706742	11	50	theme	immunohistochemistry	1300:1319	arg1	staining					1321:1328	immunohistochemistry staining	1300:1328	immunohistochemistry staining	1300:1328	In addition, immunohistochemistry staining found decreased numbers of cell proliferation (PCNA) and inflammatory cell (granulocytes) infiltration in the colon mucosa of the BF group.
30706742	2	51	theme	gut	366:368	arg1	microflora					370:379	gut microflora	366:379	gut microflora	366:379	Previous studies have indicated that the composition of gut microflora may be involved in CAC induction and progress.
30706742	9	52	theme	tumor	1090:1094	arg1	number					1096:1101	tumor number	1090:1101	tumor number	1090:1101	Interestingly, there was a significant decrease in tumor number and tumor incidence in the BF/AOM/DSS group as compared to the GF/AOM/DSS group.
30706742	14	53	theme	immunophysiologic	1851:1867	arg1	balance					1869:1875	the gastrointestinal immunophysiologic balance	1830:1875	the gastrointestinal immunophysiologic balance	1830:1875	Conclusions: Symbiotic BF may play a pivotal role in maintaining the gastrointestinal immunophysiologic balance and regulating anti-tumorigenesis responses.
30706742	13	54	theme	proliferation-related	1686:1706	arg1	molecules					1708:1716	cell proliferation-related molecules	1681:1716	cell proliferation-related molecules	1681:1716	Taking together, the BF colonization significantly ameliorated AOM/DSS-induced CRC by suppressing the activity of cell proliferation-related molecules and reducing the number of inflammatory cells.
30706742	11	55	theme	BF	1460:1461	arg1	group					1463:1467	the BF group	1456:1467	the BF group	1456:1467	In addition, immunohistochemistry staining found decreased numbers of cell proliferation (PCNA) and inflammatory cell (granulocytes) infiltration in the colon mucosa of the BF group.
30706742	11	56	theme	group	1463:1467	arg1	mucosa					1446:1451	the colon mucosa	1436:1451	the colon mucosa of the BF group	1436:1467	In addition, immunohistochemistry staining found decreased numbers of cell proliferation (PCNA) and inflammatory cell (granulocytes) infiltration in the colon mucosa of the BF group.
30706742	12	57	theme	fewer	1521:1525	arg1	expressions					1554:1564	fewer and weaker positive signal expressions	1521:1564	fewer and weaker positive signal expressions	1521:1564	The β-catenin staining in the BF/AOM/DSS group had fewer and weaker positive signal expressions.
30706742	5	58	dep	Materials	719:727	arg1	divided					764:770	divided	764:770	were divided into two groups: GF and BF group	759:803	Materials and methods: Total 22 GF mice were divided into two groups: GF and BF group.
30706742	4	59	theme	dextran	666:672	arg1	DSS					690:692	dextran sulfate sodium (DSS)	666:693	dextran sulfate sodium (DSS)	666:693	This study was aimed to investigate the protective role of BF in a colorectal cancer (CRC) model induced by azoxymethane (AOM) and dextran sulfate sodium (DSS) in germ-free (GF) mice.
30706742	14	60	theme	gastrointestinal	1834:1849	arg1	balance					1869:1875	the gastrointestinal immunophysiologic balance	1830:1875	the gastrointestinal immunophysiologic balance	1830:1875	Conclusions: Symbiotic BF may play a pivotal role in maintaining the gastrointestinal immunophysiologic balance and regulating anti-tumorigenesis responses.
30706742	1	61	theme	risk	232:235	arg1	disease					183:189	Inflammatory bowel disease	164:189	Inflammatory bowel disease (IBD)	164:195	Objective: Inflammatory bowel disease (IBD) is generally considered as a major risk factor in the progression of colitis-associated colorectal cancer (CAC).
30706742	1	61	theme	risk	232:235	arg1	factor					237:242	a major risk factor	224:242	a major risk factor in the progression of colitis-associated colorectal cancer (CAC)	224:307	Objective: Inflammatory bowel disease (IBD) is generally considered as a major risk factor in the progression of colitis-associated colorectal cancer (CAC).
30706742	12	62	theme	BF/AOM/DSS	1500:1509	arg1	group					1511:1515	the BF/AOM/DSS group	1496:1515	the BF/AOM/DSS group	1496:1515	The β-catenin staining in the BF/AOM/DSS group had fewer and weaker positive signal expressions.
30706742	1	63	from	factor	237:242	arg1	progression					251:261	the progression	247:261	the progression of colitis-associated colorectal cancer (CAC)	247:307	Objective: Inflammatory bowel disease (IBD) is generally considered as a major risk factor in the progression of colitis-associated colorectal cancer (CAC).
30706742	14	64	theme	pivotal	1802:1808	arg1	role					1810:1813	a pivotal role	1800:1813	a pivotal role	1800:1813	Conclusions: Symbiotic BF may play a pivotal role in maintaining the gastrointestinal immunophysiologic balance and regulating anti-tumorigenesis responses.
30706742	4	65	theme	sodium	682:687	arg1	DSS					690:692	dextran sulfate sodium (DSS)	666:693	dextran sulfate sodium (DSS)	666:693	This study was aimed to investigate the protective role of BF in a colorectal cancer (CRC) model induced by azoxymethane (AOM) and dextran sulfate sodium (DSS) in germ-free (GF) mice.
30706742	7	66	theme	animal	929:934	arg1	survival					936:943	animal survival	929:943	animal survival	929:943	Results: BF colonization increased animal survival (100%).
30706742	7	67	theme	BF	903:904	arg1	colonization					906:917	BF colonization	903:917	BF colonization	903:917	Results: BF colonization increased animal survival (100%).
30706742	1	68	theme	Inflammatory	164:175	arg1	IBD					192:194	IBD	192:194	IBD	192:194	Objective: Inflammatory bowel disease (IBD) is generally considered as a major risk factor in the progression of colitis-associated colorectal cancer (CAC).
30706742	1	68	theme	Inflammatory	164:175	arg1	disease					183:189	Inflammatory bowel disease	164:189	Inflammatory bowel disease (IBD)	164:195	Objective: Inflammatory bowel disease (IBD) is generally considered as a major risk factor in the progression of colitis-associated colorectal cancer (CAC).
30706742	1	68	theme	Inflammatory	164:175	arg1	factor					237:242	a major risk factor	224:242	a major risk factor in the progression of colitis-associated colorectal cancer (CAC)	224:307	Objective: Inflammatory bowel disease (IBD) is generally considered as a major risk factor in the progression of colitis-associated colorectal cancer (CAC).
30706742	11	69	theme	inflammatory	1387:1398	arg1	infiltration					1420:1431	inflammatory cell (granulocytes) infiltration	1387:1431	inflammatory cell (granulocytes) infiltration	1387:1431	In addition, immunohistochemistry staining found decreased numbers of cell proliferation (PCNA) and inflammatory cell (granulocytes) infiltration in the colon mucosa of the BF group.
30706742	0	70	theme	sulfate	92:98	arg1	sodium					100:105	azoxymethane/dextran sulfate sodium	71:105	azoxymethane/dextran sulfate sodium induced colitis-associated colorectal cancer	71:150	The germ-free mice monocolonization with Bacteroides fragilis improves azoxymethane/dextran sulfate sodium induced colitis-associated colorectal cancer.
30706742	9	71	from	decrease	1078:1085	arg1	group					1141:1145	the BF/AOM/DSS group	1126:1145	the BF/AOM/DSS group	1126:1145	Interestingly, there was a significant decrease in tumor number and tumor incidence in the BF/AOM/DSS group as compared to the GF/AOM/DSS group.
30706742	9	71	from	decrease	1078:1085	arg1	number					1096:1101	tumor number	1090:1101	tumor number	1090:1101	Interestingly, there was a significant decrease in tumor number and tumor incidence in the BF/AOM/DSS group as compared to the GF/AOM/DSS group.
30706742	9	71	from	decrease	1078:1085	arg1	incidence					1113:1121	tumor incidence	1107:1121	tumor incidence	1107:1121	Interestingly, there was a significant decrease in tumor number and tumor incidence in the BF/AOM/DSS group as compared to the GF/AOM/DSS group.
30706742	0	72	theme	colorectal	134:143	arg1	cancer					145:150	colitis-associated colorectal cancer	115:150	colitis-associated colorectal cancer	115:150	The germ-free mice monocolonization with Bacteroides fragilis improves azoxymethane/dextran sulfate sodium induced colitis-associated colorectal cancer.
30706742	11	73	theme	cell	1357:1360	arg1	PCNA					1377:1380	PCNA	1377:1380	PCNA	1377:1380	In addition, immunohistochemistry staining found decreased numbers of cell proliferation (PCNA) and inflammatory cell (granulocytes) infiltration in the colon mucosa of the BF group.
30706742	11	73	theme	cell	1357:1360	arg1	proliferation					1362:1374	cell proliferation	1357:1374	cell proliferation (PCNA)	1357:1381	In addition, immunohistochemistry staining found decreased numbers of cell proliferation (PCNA) and inflammatory cell (granulocytes) infiltration in the colon mucosa of the BF group.
30706742	0	74	theme	germ-free	4:12	arg1	mice					14:17	germ-free mice	4:17	The germ-free mice monocolonization with Bacteroides fragilis	0:60	The germ-free mice monocolonization with Bacteroides fragilis improves azoxymethane/dextran sulfate sodium induced colitis-associated colorectal cancer.
30706742	8	75	theme	Cecum	953:957	arg1	weight					959:964	Cecum weight	953:964	Cecum weight	953:964	Cecum weight and cecum/body weight ratio significantly decreased in BF/AOM/DSS group.
30706742	10	76	theme	histologic	1225:1234	arg1	score					1236:1240	histologic score	1225:1240	histologic score	1225:1240	The adenocarcinoma/adenoma incidence and histologic score were also decreased in the BF/AOM/DSS group.
30706742	4	77	theme	protective	575:584	arg1	role					586:589	the protective role	571:589	the protective role of BF in a colorectal cancer (CRC) model induced by azoxymethane (AOM) and dextran sulfate sodium (DSS) in germ-free (GF) mice	571:716	This study was aimed to investigate the protective role of BF in a colorectal cancer (CRC) model induced by azoxymethane (AOM) and dextran sulfate sodium (DSS) in germ-free (GF) mice.
30706742	0	78	theme	Bacteroides	41:51	arg1	fragilis					53:60	Bacteroides fragilis	41:60	Bacteroides fragilis	41:60	The germ-free mice monocolonization with Bacteroides fragilis improves azoxymethane/dextran sulfate sodium induced colitis-associated colorectal cancer.
30706742	8	79	theme	weight	981:986	arg1	ratio					988:992	cecum/body weight ratio	970:992	cecum/body weight ratio	970:992	Cecum weight and cecum/body weight ratio significantly decreased in BF/AOM/DSS group.
30706742	12	80	theme	positive	1538:1545	arg1	expressions					1554:1564	fewer and weaker positive signal expressions	1521:1564	fewer and weaker positive signal expressions	1521:1564	The β-catenin staining in the BF/AOM/DSS group had fewer and weaker positive signal expressions.
30706742	0	81	with	monocolonization	19:34	arg1	fragilis					53:60	Bacteroides fragilis	41:60	Bacteroides fragilis	41:60	The germ-free mice monocolonization with Bacteroides fragilis improves azoxymethane/dextran sulfate sodium induced colitis-associated colorectal cancer.
30706742	3	82	theme	Gram-negative	459:471	arg1	anaerobe					473:480	a Gram-negative anaerobe	457:480	a Gram-negative anaerobe belonging to colonic symbiotic bacteria of the host	457:532	Bacteroides fragilis (BF) is a Gram-negative anaerobe belonging to colonic symbiotic bacteria of the host.
30706742	3	82	theme	Gram-negative	459:471	arg1	fragilis					440:447	Bacteroides fragilis	428:447	Bacteroides fragilis (BF)	428:452	Bacteroides fragilis (BF) is a Gram-negative anaerobe belonging to colonic symbiotic bacteria of the host.
30706742	12	83	from	staining	1484:1491	arg1	group					1511:1515	the BF/AOM/DSS group	1496:1515	the BF/AOM/DSS group	1496:1515	The β-catenin staining in the BF/AOM/DSS group had fewer and weaker positive signal expressions.
30706742	4	84	theme	colorectal	602:611	arg1	CRC					621:623	CRC	621:623	CRC	621:623	This study was aimed to investigate the protective role of BF in a colorectal cancer (CRC) model induced by azoxymethane (AOM) and dextran sulfate sodium (DSS) in germ-free (GF) mice.
30706742	4	84	theme	colorectal	602:611	arg1	cancer					613:618	a colorectal cancer	600:618	a colorectal cancer (CRC) model induced by azoxymethane (AOM) and dextran sulfate sodium (DSS) in germ-free (GF) mice	600:716	This study was aimed to investigate the protective role of BF in a colorectal cancer (CRC) model induced by azoxymethane (AOM) and dextran sulfate sodium (DSS) in germ-free (GF) mice.
30706742	5	85	dep	groups	781:786	arg1	GF					789:790	GF	789:790	GF	789:790	Materials and methods: Total 22 GF mice were divided into two groups: GF and BF group.
30706742	5	85	dep	groups	781:786	arg1	group					799:803	BF group	796:803	BF group	796:803	Materials and methods: Total 22 GF mice were divided into two groups: GF and BF group.
30706742	5	85	dep	groups	781:786	arg1	groups					781:786	two groups	777:786	two groups: GF and BF group	777:803	Materials and methods: Total 22 GF mice were divided into two groups: GF and BF group.
30706742	12	86	contain	had	1517:1519	arg1	staining					1484:1491	The β-catenin staining	1470:1491	The β-catenin staining in the BF/AOM/DSS group	1470:1515	The β-catenin staining in the BF/AOM/DSS group had fewer and weaker positive signal expressions.
30706742	12	86	contain	had	1517:1519	arg2	expressions					1554:1564	fewer and weaker positive signal expressions	1521:1564	fewer and weaker positive signal expressions	1521:1564	The β-catenin staining in the BF/AOM/DSS group had fewer and weaker positive signal expressions.
30706742	3	87	theme	colonic	495:501	arg1	bacteria					513:520	colonic symbiotic bacteria	495:520	colonic symbiotic bacteria of the host	495:532	Bacteroides fragilis (BF) is a Gram-negative anaerobe belonging to colonic symbiotic bacteria of the host.
30706742	14	88	theme	anti-tumorigenesis	1892:1909	arg1	responses					1911:1919	anti-tumorigenesis responses	1892:1919	anti-tumorigenesis responses	1892:1919	Conclusions: Symbiotic BF may play a pivotal role in maintaining the gastrointestinal immunophysiologic balance and regulating anti-tumorigenesis responses.
30706742	13	89	theme	inflammatory	1745:1756	arg1	cells					1758:1762	inflammatory cells	1745:1762	inflammatory cells	1745:1762	Taking together, the BF colonization significantly ameliorated AOM/DSS-induced CRC by suppressing the activity of cell proliferation-related molecules and reducing the number of inflammatory cells.
30461378	2	0	theme	safensis	107:114	arg1	subsp					116:120	Bacillus safensis subsp	98:120	Bacillus safensis subsp	98:120	nov., isolated from condensed milk, and description of Bacillus safensis subsp.
30461378	13	1	theme	Meso-diaminopimelic	1512:1530	arg1	acid					1532:1535	Meso-diaminopimelic acid	1512:1535	Meso-diaminopimelic acid	1512:1535	Meso-diaminopimelic acid was detected in the peptidoglycan.
30461378	10	2	theme	phenotypic	1176:1185	arg1	characteristics					1187:1201	several phenotypic characteristics	1168:1201	several phenotypic characteristics	1168:1201	However, strain BC09T differs from the type strains of its closest relatives in several phenotypic characteristics.
30461378	9	3	theme	Average	867:873	arg1	values					901:906	Average nucleotide identity blast values	867:906	Average nucleotide identity blast values obtained for BC09T compared to the closest relative type strains	867:971	Average nucleotide identity blast values obtained for BC09T compared to the closest relative type strains were 95.7 and 67.6 %, respectively, and predicted DNA-DNA hybridization values were 93.1 and 51.9 %, respectively.
30461378	2	4	theme	Bacillus	98:105	arg1	subsp					116:120	Bacillus safensis subsp	98:120	Bacillus safensis subsp	98:120	nov., isolated from condensed milk, and description of Bacillus safensis subsp.
30461378	10	5	theme	several	1168:1174	arg1	characteristics					1187:1201	several phenotypic characteristics	1168:1201	several phenotypic characteristics	1168:1201	However, strain BC09T differs from the type strains of its closest relatives in several phenotypic characteristics.
30461378	10	6	theme	type	1127:1130	arg1	strains					1132:1138	the type strains	1123:1138	the type strains of its closest relatives in several phenotypic characteristics	1123:1201	However, strain BC09T differs from the type strains of its closest relatives in several phenotypic characteristics.
30461378	9	7	theme	nucleotide	875:884	arg1	values					901:906	Average nucleotide identity blast values	867:906	Average nucleotide identity blast values obtained for BC09T compared to the closest relative type strains	867:971	Average nucleotide identity blast values obtained for BC09T compared to the closest relative type strains were 95.7 and 67.6 %, respectively, and predicted DNA-DNA hybridization values were 93.1 and 51.9 %, respectively.
30461378	10	8	theme	strain	1097:1102	arg1	BC09T					1104:1108	strain BC09T	1097:1108	strain BC09T	1097:1108	However, strain BC09T differs from the type strains of its closest relatives in several phenotypic characteristics.
30461378	15	9	theme	new	1694:1696	arg1	subspecies					1698:1707	a new subspecies	1692:1707	a new subspecies	1692:1707	Phylogenetic, chemotaxonomic and phenotypic analyses showed that strain BC09T represents a new subspecies of B. safensis, for which the name Bacillus safensis subsp.
30461378	6	10	theme	rRNA	276:279	arg1	sequences					286:294	16S rRNA gene sequences	272:294	16S rRNA gene sequences	272:294	Phylogenetic analyses based on 16S rRNA gene sequences placed strain BC09T into the genus Bacillus with its closest relatives being Bacillus safensis and Bacillus australimaris with 100 and 99.9 % similarity, respectively.
30461378	9	11	theme	identity	886:893	arg1	values					901:906	Average nucleotide identity blast values	867:906	Average nucleotide identity blast values obtained for BC09T compared to the closest relative type strains	867:971	Average nucleotide identity blast values obtained for BC09T compared to the closest relative type strains were 95.7 and 67.6 %, respectively, and predicted DNA-DNA hybridization values were 93.1 and 51.9 %, respectively.
30461378	5	12	theme	condensed	225:233	arg1	milk					235:238	condensed milk	225:238	condensed milk	225:238	A bacterial strain, designated BC09T, was isolated from a contaminated sample of condensed milk.
30461378	18	13	theme	type	1809:1812	arg1	BC09T					1824:1828	BC09T	1824:1828	BC09T (=LMG 30124T, =CECT 9344T)	1824:1855	The type strain is BC09T (=LMG 30124T, =CECT 9344T).
30461378	18	13	theme	type	1809:1812	arg1	strain					1814:1819	The type strain	1805:1819	The type strain	1805:1819	The type strain is BC09T (=LMG 30124T, =CECT 9344T).
30461378	7	14	theme	safensis	664:671	arg1	strains					650:656	the type strains	641:656	the type strains of B. safensis and B. australimaris	641:692	Analysis of the gyrB gene confirmed the closeness of strain BC09T with respect to the species B. safensis since it presented 97.8 and 95.2 % similarity values, respectively, to the type strains of B. safensis and B. australimaris.
30461378	9	15	theme	blast	895:899	arg1	values					901:906	Average nucleotide identity blast values	867:906	Average nucleotide identity blast values obtained for BC09T compared to the closest relative type strains	867:971	Average nucleotide identity blast values obtained for BC09T compared to the closest relative type strains were 95.7 and 67.6 %, respectively, and predicted DNA-DNA hybridization values were 93.1 and 51.9 %, respectively.
30461378	5	16	theme	milk	235:238	arg1	sample					215:220	a contaminated sample	200:220	a contaminated sample of condensed milk	200:238	A bacterial strain, designated BC09T, was isolated from a contaminated sample of condensed milk.
30461378	18	17	theme	=CECT	1844:1848	arg1	30124T					1836:1841	=LMG 30124T	1831:1841	=LMG 30124T	1831:1841	The type strain is BC09T (=LMG 30124T, =CECT 9344T).
30461378	18	17	theme	=CECT	1844:1848	arg1	9344T					1850:1854	=CECT 9344T	1844:1854	=CECT 9344T	1844:1854	The type strain is BC09T (=LMG 30124T, =CECT 9344T).
30461378	15	18	theme	safensis	1715:1722	arg1	subspecies					1698:1707	a new subspecies	1692:1707	a new subspecies	1692:1707	Phylogenetic, chemotaxonomic and phenotypic analyses showed that strain BC09T represents a new subspecies of B. safensis, for which the name Bacillus safensis subsp.
30461378	10	19	from	relatives	1155:1163	arg1	characteristics					1187:1201	several phenotypic characteristics	1168:1201	several phenotypic characteristics	1168:1201	However, strain BC09T differs from the type strains of its closest relatives in several phenotypic characteristics.
30461378	18	20	dep	BC09T	1824:1828	arg1	30124T					1836:1841	=LMG 30124T	1831:1841	=LMG 30124T	1831:1841	The type strain is BC09T (=LMG 30124T, =CECT 9344T).
30461378	18	20	dep	BC09T	1824:1828	arg1	9344T					1850:1854	=CECT 9344T	1844:1854	=CECT 9344T	1844:1854	The type strain is BC09T (=LMG 30124T, =CECT 9344T).
30461378	11	21	theme	major	1284:1288	arg1	anteiso-C15:0					1261:1273	anteiso-C15:0	1261:1273	anteiso-C15:0	1261:1273	MK-7 was the only menaquinone detected and iso-C15:0 and anteiso-C15:0 were the major fatty acids.
30461378	11	21	theme	major	1284:1288	arg1	iso-C15:0					1247:1255	iso-C15:0	1247:1255	iso-C15:0	1247:1255	MK-7 was the only menaquinone detected and iso-C15:0 and anteiso-C15:0 were the major fatty acids.
30461378	11	21	theme	major	1284:1288	arg1	acids					1296:1300	the major fatty acids	1280:1300	the major fatty acids	1280:1300	MK-7 was the only menaquinone detected and iso-C15:0 and anteiso-C15:0 were the major fatty acids.
30461378	16	22	dep	subsp	1780:1784	arg1	osmophilus					1769:1778	osmophilus	1769:1778	osmophilus	1769:1778	osmophilus subsp.
30461378	6	23	theme	strain	303:308	arg1	BC09T					310:314	strain BC09T	303:314	strain BC09T	303:314	Phylogenetic analyses based on 16S rRNA gene sequences placed strain BC09T into the genus Bacillus with its closest relatives being Bacillus safensis and Bacillus australimaris with 100 and 99.9 % similarity, respectively.
30461378	10	24	theme	relatives	1155:1163	arg1	strains					1132:1138	the type strains	1123:1138	the type strains of its closest relatives in several phenotypic characteristics	1123:1201	However, strain BC09T differs from the type strains of its closest relatives in several phenotypic characteristics.
30461378	6	25	dep	similarity	438:447	arg1	%					436:436	%	436:436	%	436:436	Phylogenetic analyses based on 16S rRNA gene sequences placed strain BC09T into the genus Bacillus with its closest relatives being Bacillus safensis and Bacillus australimaris with 100 and 99.9 % similarity, respectively.
30461378	8	26	theme	DNA-DNA	695:701	arg1	hybridization					703:715	DNA-DNA hybridization	695:715	DNA-DNA hybridization	695:715	DNA-DNA hybridization confirmed these results showing averages of 67 and 56 %, respectively, between strain BC09T and the type strains of B. safensis and B. australimaris.
30461378	11	27	theme	only	1217:1220	arg1	menaquinone					1222:1232	the only menaquinone	1213:1232	the only menaquinone	1213:1232	MK-7 was the only menaquinone detected and iso-C15:0 and anteiso-C15:0 were the major fatty acids.
30461378	14	28	theme	mol	1597:1599	arg1	content					1580:1586	The G+C content	1572:1586	The G+C content	1572:1586	The G+C content was 40.9 mol%.
30461378	14	28	theme	mol	1597:1599	arg1	%					1600:1600	40.9 mol%	1592:1600	40.9 mol%	1592:1600	The G+C content was 40.9 mol%.
30461378	8	29	theme	type	817:820	arg1	strains					822:828	the type strains	813:828	the type strains of B. safensis and B. australimaris	813:864	DNA-DNA hybridization confirmed these results showing averages of 67 and 56 %, respectively, between strain BC09T and the type strains of B. safensis and B. australimaris.
30461378	6	30	dep	%	436:436	arg1	100					423:425	100	423:425	100	423:425	Phylogenetic analyses based on 16S rRNA gene sequences placed strain BC09T into the genus Bacillus with its closest relatives being Bacillus safensis and Bacillus australimaris with 100 and 99.9 % similarity, respectively.
30461378	6	30	dep	%	436:436	arg1	99.9 					431:435	99.9 	431:435	99.9 	431:435	Phylogenetic analyses based on 16S rRNA gene sequences placed strain BC09T into the genus Bacillus with its closest relatives being Bacillus safensis and Bacillus australimaris with 100 and 99.9 % similarity, respectively.
30461378	2	31	theme	condensed	63:71	arg1	milk					73:76	condensed milk	63:76	condensed milk	63:76	nov., isolated from condensed milk, and description of Bacillus safensis subsp.
30461378	12	32	theme	unidentified	1390:1401	arg1	phospholipids					1403:1415	two unidentified phospholipids	1386:1415	two unidentified phospholipids	1386:1415	The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, two unidentified phospholipids, two unidentifed glycolipids, three unidentified lipids and one unidentifed phosphoglycolipid.
30461378	12	32	theme	unidentified	1390:1401	arg1	diphosphatidylglycerol					1340:1361	diphosphatidylglycerol	1340:1361	diphosphatidylglycerol	1340:1361	The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, two unidentified phospholipids, two unidentifed glycolipids, three unidentified lipids and one unidentifed phosphoglycolipid.
30461378	9	33	theme	DNA-DNA	1023:1029	arg1	hybridization					1031:1043	DNA-DNA hybridization	1023:1043	predicted DNA-DNA hybridization values	1013:1050	Average nucleotide identity blast values obtained for BC09T compared to the closest relative type strains were 95.7 and 67.6 %, respectively, and predicted DNA-DNA hybridization values were 93.1 and 51.9 %, respectively.
30461378	12	34	theme	unidentified	1453:1464	arg1	lipids					1466:1471	three unidentified lipids	1447:1471	three unidentified lipids	1447:1471	The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, two unidentified phospholipids, two unidentifed glycolipids, three unidentified lipids and one unidentifed phosphoglycolipid.
30461378	12	34	theme	unidentified	1453:1464	arg1	diphosphatidylglycerol					1340:1361	diphosphatidylglycerol	1340:1361	diphosphatidylglycerol	1340:1361	The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, two unidentified phospholipids, two unidentifed glycolipids, three unidentified lipids and one unidentifed phosphoglycolipid.
30461378	7	35	dep	%	603:603	arg1	97.8					589:592	97.8	589:592	97.8	589:592	Analysis of the gyrB gene confirmed the closeness of strain BC09T with respect to the species B. safensis since it presented 97.8 and 95.2 % similarity values, respectively, to the type strains of B. safensis and B. australimaris.
30461378	7	35	dep	%	603:603	arg1	95.2 					598:602	95.2 	598:602	95.2 	598:602	Analysis of the gyrB gene confirmed the closeness of strain BC09T with respect to the species B. safensis since it presented 97.8 and 95.2 % similarity values, respectively, to the type strains of B. safensis and B. australimaris.
30461378	6	36	theme	gene	281:284	arg1	sequences					286:294	16S rRNA gene sequences	272:294	16S rRNA gene sequences	272:294	Phylogenetic analyses based on 16S rRNA gene sequences placed strain BC09T into the genus Bacillus with its closest relatives being Bacillus safensis and Bacillus australimaris with 100 and 99.9 % similarity, respectively.
30461378	9	37	theme	hybridization	1031:1043	arg1	values					1045:1050	predicted DNA-DNA hybridization values	1013:1050	predicted DNA-DNA hybridization values	1013:1050	Average nucleotide identity blast values obtained for BC09T compared to the closest relative type strains were 95.7 and 67.6 %, respectively, and predicted DNA-DNA hybridization values were 93.1 and 51.9 %, respectively.
30461378	13	38	located	detected	1541:1548	arg1	peptidoglycan					1557:1569	the peptidoglycan	1553:1569	the peptidoglycan	1553:1569	Meso-diaminopimelic acid was detected in the peptidoglycan.
30461378	13	38	located	detected	1541:1548	arg2	acid					1532:1535	Meso-diaminopimelic acid	1512:1535	Meso-diaminopimelic acid	1512:1535	Meso-diaminopimelic acid was detected in the peptidoglycan.
30461378	9	39	theme	predicted	1013:1021	arg1	values					1045:1050	predicted DNA-DNA hybridization values	1013:1050	predicted DNA-DNA hybridization values	1013:1050	Average nucleotide identity blast values obtained for BC09T compared to the closest relative type strains were 95.7 and 67.6 %, respectively, and predicted DNA-DNA hybridization values were 93.1 and 51.9 %, respectively.
30461378	2	40	attach	isolated	49:56	arg2	nov.					43:46	nov.	43:46	nov.	43:46	nov., isolated from condensed milk, and description of Bacillus safensis subsp.
30461378	2	40	attach	isolated	49:56	arg1	milk					73:76	condensed milk	63:76	condensed milk	63:76	nov., isolated from condensed milk, and description of Bacillus safensis subsp.
30461378	15	41	theme	name	1739:1742	arg1	subsp					1762:1766	the name Bacillus safensis subsp	1735:1766	the name Bacillus safensis subsp	1735:1766	Phylogenetic, chemotaxonomic and phenotypic analyses showed that strain BC09T represents a new subspecies of B. safensis, for which the name Bacillus safensis subsp.
30461378	11	42	theme	fatty	1290:1294	arg1	anteiso-C15:0					1261:1273	anteiso-C15:0	1261:1273	anteiso-C15:0	1261:1273	MK-7 was the only menaquinone detected and iso-C15:0 and anteiso-C15:0 were the major fatty acids.
30461378	11	42	theme	fatty	1290:1294	arg1	iso-C15:0					1247:1255	iso-C15:0	1247:1255	iso-C15:0	1247:1255	MK-7 was the only menaquinone detected and iso-C15:0 and anteiso-C15:0 were the major fatty acids.
30461378	11	42	theme	fatty	1290:1294	arg1	acids					1296:1300	the major fatty acids	1280:1300	the major fatty acids	1280:1300	MK-7 was the only menaquinone detected and iso-C15:0 and anteiso-C15:0 were the major fatty acids.
30461378	7	43	dep	values	616:621	arg1	%					603:603	%	603:603	%	603:603	Analysis of the gyrB gene confirmed the closeness of strain BC09T with respect to the species B. safensis since it presented 97.8 and 95.2 % similarity values, respectively, to the type strains of B. safensis and B. australimaris.
30461378	5	44	theme	contaminated	202:213	arg1	sample					215:220	a contaminated sample	200:220	a contaminated sample of condensed milk	200:238	A bacterial strain, designated BC09T, was isolated from a contaminated sample of condensed milk.
30461378	7	45	theme	strain	517:522	arg1	BC09T					524:528	strain BC09T	517:528	strain BC09T	517:528	Analysis of the gyrB gene confirmed the closeness of strain BC09T with respect to the species B. safensis since it presented 97.8 and 95.2 % similarity values, respectively, to the type strains of B. safensis and B. australimaris.
30461378	8	46	theme	safensis	836:843	arg1	strains					822:828	the type strains	813:828	the type strains of B. safensis and B. australimaris	813:864	DNA-DNA hybridization confirmed these results showing averages of 67 and 56 %, respectively, between strain BC09T and the type strains of B. safensis and B. australimaris.
30461378	8	46	theme	safensis	836:843	arg1	averages					749:756	averages	749:756	averages of 67 and 56 %, respectively, between strain BC09T	749:807	DNA-DNA hybridization confirmed these results showing averages of 67 and 56 %, respectively, between strain BC09T and the type strains of B. safensis and B. australimaris.
30461378	9	47	theme	type	960:963	arg1	strains					965:971	the closest relative type strains	939:971	the closest relative type strains	939:971	Average nucleotide identity blast values obtained for BC09T compared to the closest relative type strains were 95.7 and 67.6 %, respectively, and predicted DNA-DNA hybridization values were 93.1 and 51.9 %, respectively.
30461378	12	48	theme	lipid	1313:1317	arg1	profile					1319:1325	The polar lipid profile	1303:1325	The polar lipid profile	1303:1325	The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, two unidentified phospholipids, two unidentifed glycolipids, three unidentified lipids and one unidentifed phosphoglycolipid.
30461378	7	49	theme	BC09T	524:528	arg1	closeness					504:512	the closeness	500:512	the closeness of strain BC09T with respect to the species	500:556	Analysis of the gyrB gene confirmed the closeness of strain BC09T with respect to the species B. safensis since it presented 97.8 and 95.2 % similarity values, respectively, to the type strains of B. safensis and B. australimaris.
30461378	8	50	theme	australimaris	852:864	arg1	strains					822:828	the type strains	813:828	the type strains of B. safensis and B. australimaris	813:864	DNA-DNA hybridization confirmed these results showing averages of 67 and 56 %, respectively, between strain BC09T and the type strains of B. safensis and B. australimaris.
30461378	8	50	theme	australimaris	852:864	arg1	averages					749:756	averages	749:756	averages of 67 and 56 %, respectively, between strain BC09T	749:807	DNA-DNA hybridization confirmed these results showing averages of 67 and 56 %, respectively, between strain BC09T and the type strains of B. safensis and B. australimaris.
30461378	5	51	attach	isolated	186:193	arg1	sample					215:220	a contaminated sample	200:220	a contaminated sample of condensed milk	200:238	A bacterial strain, designated BC09T, was isolated from a contaminated sample of condensed milk.
30461378	5	51	attach	isolated	186:193	arg2	strain					156:161	A bacterial strain	144:161	A bacterial strain	144:161	A bacterial strain, designated BC09T, was isolated from a contaminated sample of condensed milk.
30461378	9	52	theme	closest	943:949	arg1	strains					965:971	the closest relative type strains	939:971	the closest relative type strains	939:971	Average nucleotide identity blast values obtained for BC09T compared to the closest relative type strains were 95.7 and 67.6 %, respectively, and predicted DNA-DNA hybridization values were 93.1 and 51.9 %, respectively.
30461378	12	53	theme	unidentifed	1481:1491	arg1	phosphoglycolipid					1493:1509	one unidentifed phosphoglycolipid	1477:1509	one unidentifed phosphoglycolipid	1477:1509	The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, two unidentified phospholipids, two unidentifed glycolipids, three unidentified lipids and one unidentifed phosphoglycolipid.
30461378	12	53	theme	unidentifed	1481:1491	arg1	diphosphatidylglycerol					1340:1361	diphosphatidylglycerol	1340:1361	diphosphatidylglycerol	1340:1361	The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, two unidentified phospholipids, two unidentifed glycolipids, three unidentified lipids and one unidentifed phosphoglycolipid.
30461378	7	54	theme	type	645:648	arg1	strains					650:656	the type strains	641:656	the type strains of B. safensis and B. australimaris	641:692	Analysis of the gyrB gene confirmed the closeness of strain BC09T with respect to the species B. safensis since it presented 97.8 and 95.2 % similarity values, respectively, to the type strains of B. safensis and B. australimaris.
30461378	15	55	theme	phenotypic	1636:1645	arg1	analyses					1647:1654	Phylogenetic, chemotaxonomic and phenotypic analyses	1603:1654	Phylogenetic, chemotaxonomic and phenotypic analyses	1603:1654	Phylogenetic, chemotaxonomic and phenotypic analyses showed that strain BC09T represents a new subspecies of B. safensis, for which the name Bacillus safensis subsp.
30461378	6	56	theme	16S	272:274	arg1	sequences					286:294	16S rRNA gene sequences	272:294	16S rRNA gene sequences	272:294	Phylogenetic analyses based on 16S rRNA gene sequences placed strain BC09T into the genus Bacillus with its closest relatives being Bacillus safensis and Bacillus australimaris with 100 and 99.9 % similarity, respectively.
30461378	9	57	theme	relative	951:958	arg1	strains					965:971	the closest relative type strains	939:971	the closest relative type strains	939:971	Average nucleotide identity blast values obtained for BC09T compared to the closest relative type strains were 95.7 and 67.6 %, respectively, and predicted DNA-DNA hybridization values were 93.1 and 51.9 %, respectively.
30461378	8	58	theme	strain	796:801	arg1	BC09T					803:807	strain BC09T	796:807	strain BC09T	796:807	DNA-DNA hybridization confirmed these results showing averages of 67 and 56 %, respectively, between strain BC09T and the type strains of B. safensis and B. australimaris.
30461378	2	59	theme	subsp	116:120	arg1	description					83:93	description	83:93	description of Bacillus safensis subsp	83:120	nov., isolated from condensed milk, and description of Bacillus safensis subsp.
30461378	2	59	theme	subsp	116:120	arg1	nov.					43:46	nov.	43:46	nov.	43:46	nov., isolated from condensed milk, and description of Bacillus safensis subsp.
30461378	6	60	theme	closest	349:355	arg1	relatives					357:365	its closest relatives	345:365	its closest relatives	345:365	Phylogenetic analyses based on 16S rRNA gene sequences placed strain BC09T into the genus Bacillus with its closest relatives being Bacillus safensis and Bacillus australimaris with 100 and 99.9 % similarity, respectively.
30461378	8	61	theme	%	771:771	arg1	strains					822:828	the type strains	813:828	the type strains of B. safensis and B. australimaris	813:864	DNA-DNA hybridization confirmed these results showing averages of 67 and 56 %, respectively, between strain BC09T and the type strains of B. safensis and B. australimaris.
30461378	8	61	theme	%	771:771	arg1	averages					749:756	averages	749:756	averages of 67 and 56 %, respectively, between strain BC09T	749:807	DNA-DNA hybridization confirmed these results showing averages of 67 and 56 %, respectively, between strain BC09T and the type strains of B. safensis and B. australimaris.
30461378	7	62	theme	gene	485:488	arg1	Analysis					464:471	Analysis	464:471	Analysis of the gyrB gene	464:488	Analysis of the gyrB gene confirmed the closeness of strain BC09T with respect to the species B. safensis since it presented 97.8 and 95.2 % similarity values, respectively, to the type strains of B. safensis and B. australimaris.
30461378	10	63	from	strains	1132:1138	arg1	characteristics					1187:1201	several phenotypic characteristics	1168:1201	several phenotypic characteristics	1168:1201	However, strain BC09T differs from the type strains of its closest relatives in several phenotypic characteristics.
30461378	12	64	theme	unidentifed	1422:1432	arg1	glycolipids					1434:1444	two unidentifed glycolipids	1418:1444	two unidentifed glycolipids	1418:1444	The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, two unidentified phospholipids, two unidentifed glycolipids, three unidentified lipids and one unidentifed phosphoglycolipid.
30461378	12	64	theme	unidentifed	1422:1432	arg1	diphosphatidylglycerol					1340:1361	diphosphatidylglycerol	1340:1361	diphosphatidylglycerol	1340:1361	The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, two unidentified phospholipids, two unidentifed glycolipids, three unidentified lipids and one unidentifed phosphoglycolipid.
30461378	15	65	theme	Bacillus	1744:1751	arg1	subsp					1762:1766	the name Bacillus safensis subsp	1735:1766	the name Bacillus safensis subsp	1735:1766	Phylogenetic, chemotaxonomic and phenotypic analyses showed that strain BC09T represents a new subspecies of B. safensis, for which the name Bacillus safensis subsp.
30461378	14	66	theme	G+C	1576:1578	arg1	content					1580:1586	The G+C content	1572:1586	The G+C content	1572:1586	The G+C content was 40.9 mol%.
30461378	14	66	theme	G+C	1576:1578	arg1	%					1600:1600	40.9 mol%	1592:1600	40.9 mol%	1592:1600	The G+C content was 40.9 mol%.
30461378	15	67	theme	strain	1668:1673	arg1	BC09T					1675:1679	strain BC09T	1668:1679	strain BC09T	1668:1679	Phylogenetic, chemotaxonomic and phenotypic analyses showed that strain BC09T represents a new subspecies of B. safensis, for which the name Bacillus safensis subsp.
30461378	10	68	theme	closest	1147:1153	arg1	relatives					1155:1163	its closest relatives	1143:1163	its closest relatives in several phenotypic characteristics	1143:1201	However, strain BC09T differs from the type strains of its closest relatives in several phenotypic characteristics.
30461378	12	69	theme	polar	1307:1311	arg1	profile					1319:1325	The polar lipid profile	1303:1325	The polar lipid profile	1303:1325	The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, two unidentified phospholipids, two unidentifed glycolipids, three unidentified lipids and one unidentifed phosphoglycolipid.
30461378	15	70	theme	chemotaxonomic	1617:1630	arg1	analyses					1647:1654	Phylogenetic, chemotaxonomic and phenotypic analyses	1603:1654	Phylogenetic, chemotaxonomic and phenotypic analyses	1603:1654	Phylogenetic, chemotaxonomic and phenotypic analyses showed that strain BC09T represents a new subspecies of B. safensis, for which the name Bacillus safensis subsp.
30461378	10	71	from	characteristics	1187:1201	arg1	strains					1132:1138	the type strains	1123:1138	the type strains of its closest relatives in several phenotypic characteristics	1123:1201	However, strain BC09T differs from the type strains of its closest relatives in several phenotypic characteristics.
30461378	1	72	dep	subsp	36:40	arg1	osmophilus					25:34	osmophilus	25:34	osmophilus	25:34	osmophilus subsp.
30461378	7	73	theme	gyrB	480:483	arg1	gene					485:488	the gyrB gene	476:488	the gyrB gene	476:488	Analysis of the gyrB gene confirmed the closeness of strain BC09T with respect to the species B. safensis since it presented 97.8 and 95.2 % similarity values, respectively, to the type strains of B. safensis and B. australimaris.
30461378	15	74	theme	Phylogenetic	1603:1614	arg1	analyses					1647:1654	Phylogenetic, chemotaxonomic and phenotypic analyses	1603:1654	Phylogenetic, chemotaxonomic and phenotypic analyses	1603:1654	Phylogenetic, chemotaxonomic and phenotypic analyses showed that strain BC09T represents a new subspecies of B. safensis, for which the name Bacillus safensis subsp.
30461378	6	75	theme	Phylogenetic	241:252	arg1	analyses					254:261	Phylogenetic analyses	241:261	Phylogenetic analyses based on 16S rRNA gene sequences	241:294	Phylogenetic analyses based on 16S rRNA gene sequences placed strain BC09T into the genus Bacillus with its closest relatives being Bacillus safensis and Bacillus australimaris with 100 and 99.9 % similarity, respectively.
30461378	18	76	theme	=LMG	1831:1834	arg1	30124T					1836:1841	=LMG 30124T	1831:1841	=LMG 30124T	1831:1841	The type strain is BC09T (=LMG 30124T, =CECT 9344T).
30461378	18	76	theme	=LMG	1831:1834	arg1	9344T					1850:1854	=CECT 9344T	1844:1854	=CECT 9344T	1844:1854	The type strain is BC09T (=LMG 30124T, =CECT 9344T).
30461378	7	77	theme	australimaris	680:692	arg1	strains					650:656	the type strains	641:656	the type strains of B. safensis and B. australimaris	641:692	Analysis of the gyrB gene confirmed the closeness of strain BC09T with respect to the species B. safensis since it presented 97.8 and 95.2 % similarity values, respectively, to the type strains of B. safensis and B. australimaris.
30461378	11	78	dep	detected	1234:1241	arg1	menaquinone					1222:1232	the only menaquinone	1213:1232	the only menaquinone	1213:1232	MK-7 was the only menaquinone detected and iso-C15:0 and anteiso-C15:0 were the major fatty acids.
30461378	7	79	theme	similarity	605:614	arg1	values					616:621	97.8 and 95.2 % similarity values	589:621	97.8 and 95.2 % similarity values	589:621	Analysis of the gyrB gene confirmed the closeness of strain BC09T with respect to the species B. safensis since it presented 97.8 and 95.2 % similarity values, respectively, to the type strains of B. safensis and B. australimaris.
30461378	5	80	theme	bacterial	146:154	arg1	strain					156:161	A bacterial strain	144:161	A bacterial strain	144:161	A bacterial strain, designated BC09T, was isolated from a contaminated sample of condensed milk.
30461378	15	81	dep	Bacillus	1744:1751	arg1	safensis					1753:1760	safensis	1753:1760	safensis	1753:1760	Phylogenetic, chemotaxonomic and phenotypic analyses showed that strain BC09T represents a new subspecies of B. safensis, for which the name Bacillus safensis subsp.
30758933	3	0	theme	near-infrared	682:694	arg1	irradiation					702:712	near-infrared light irradiation	682:712	near-infrared light irradiation treatment	682:722	In our study, we have developed an in vitro model to evaluate the efficacy of 2D bP material with and without near-infrared light irradiation treatment, and we have demonstrated that the presence of 2D bP without treatment inhibits the metabolic activity of osteosarcoma cells (SAOS-2) while inducing both the proliferation and the osteogenic differentiation of human preosteoblast cells (HOb) and mesenchymal stem cells.
30758933	3	1	theme	irradiation	702:712	arg1	treatment					714:722	near-infrared light irradiation treatment	682:722	near-infrared light irradiation treatment	682:722	In our study, we have developed an in vitro model to evaluate the efficacy of 2D bP material with and without near-infrared light irradiation treatment, and we have demonstrated that the presence of 2D bP without treatment inhibits the metabolic activity of osteosarcoma cells (SAOS-2) while inducing both the proliferation and the osteogenic differentiation of human preosteoblast cells (HOb) and mesenchymal stem cells.
30758933	5	2	theme	2D	1186:1187	arg1	bP					1189:1190	2D bP	1186:1190	2D bP	1186:1190	On this coculture model, 2D bP may increase anti-inflammatory cytokine generation (i.e., interleukin-10) and inhibit proinflammatory mediators synthesis (i.e., interleukin-6), thus suggesting the opportunity to prevent cancer-related inflammation.
30758933	1	3	theme	healthy	273:279	arg1	cells					292:296	healthy and cancer cells	273:296	cells	292:296	Nowadays chemotherapy is the main treatment for osteosarcoma disease, even if limited by the lack of selectivity between healthy and cancer cells during the inhibition of cell division.
30758933	0	4	theme	Osteosarcoma	79:90	arg1	Progression					92:102	Osteosarcoma Progression	79:102	Osteosarcoma Progression	79:102	Exfoliated Black Phosphorus Promotes in Vitro Bone Regeneration and Suppresses Osteosarcoma Progression through Cancer-Related Inflammation Inhibition.
30758933	4	5	theme	coculture	1035:1043	arg1	model					1045:1049	an in vitro coculture model	1023:1049	an in vitro coculture model (SAOS-2 and HOb cell lines) in order to study the effect of 2D bP on inflammatory response related to cancer	1023:1158	Furthermore, we also propose an in vitro coculture model (SAOS-2 and HOb cell lines) in order to study the effect of 2D bP on inflammatory response related to cancer.
30758933	0	6	theme	Cancer-Related	112:125	arg1	Inhibition					140:149	Cancer-Related Inflammation Inhibition	112:149	Cancer-Related Inflammation Inhibition	112:149	Exfoliated Black Phosphorus Promotes in Vitro Bone Regeneration and Suppresses Osteosarcoma Progression through Cancer-Related Inflammation Inhibition.
30758933	4	7	dep	in	1026:1027	arg1	vitro					1029:1033	vitro	1029:1033	vitro	1029:1033	Furthermore, we also propose an in vitro coculture model (SAOS-2 and HOb cell lines) in order to study the effect of 2D bP on inflammatory response related to cancer.
30758933	1	8	theme	cancer	285:290	arg1	cells					292:296	healthy and cancer cells	273:296	cells	292:296	Nowadays chemotherapy is the main treatment for osteosarcoma disease, even if limited by the lack of selectivity between healthy and cancer cells during the inhibition of cell division.
30758933	2	9	theme	bone	519:522	arg1	generation					531:540	newly forming bone tissue generation	505:540	newly forming bone tissue generation	505:540	Herein, we propose the use of few-layer two-dimensional black phosphorous (2D bP) as an alternative tool for osteosarcoma treatment and report how 2D bP can stimulate newly forming bone tissue generation after osteosarcoma resection.
30758933	3	10	theme	stem	982:985	arg1	cells					987:991	mesenchymal stem cells	970:991	mesenchymal stem cells	970:991	In our study, we have developed an in vitro model to evaluate the efficacy of 2D bP material with and without near-infrared light irradiation treatment, and we have demonstrated that the presence of 2D bP without treatment inhibits the metabolic activity of osteosarcoma cells (SAOS-2) while inducing both the proliferation and the osteogenic differentiation of human preosteoblast cells (HOb) and mesenchymal stem cells.
30758933	6	11	theme	regenerative	1494:1505	arg1	medicine					1507:1514	future regenerative medicine	1487:1514	future regenerative medicine	1487:1514	Finally, we have demonstrated that 2D bP represents a promising candidate for future regenerative medicine and anticancer applications.
30758933	5	12	dep	interleukin-6	1321:1333	arg1	i.e.					1315:1318	i.e.	1315:1318	i.e.	1315:1318	On this coculture model, 2D bP may increase anti-inflammatory cytokine generation (i.e., interleukin-10) and inhibit proinflammatory mediators synthesis (i.e., interleukin-6), thus suggesting the opportunity to prevent cancer-related inflammation.
30758933	2	13	theme	forming	511:517	arg1	generation					531:540	newly forming bone tissue generation	505:540	newly forming bone tissue generation	505:540	Herein, we propose the use of few-layer two-dimensional black phosphorous (2D bP) as an alternative tool for osteosarcoma treatment and report how 2D bP can stimulate newly forming bone tissue generation after osteosarcoma resection.
30758933	5	14	theme	cancer-related	1380:1393	arg1	inflammation					1395:1406	cancer-related inflammation	1380:1406	cancer-related inflammation	1380:1406	On this coculture model, 2D bP may increase anti-inflammatory cytokine generation (i.e., interleukin-10) and inhibit proinflammatory mediators synthesis (i.e., interleukin-6), thus suggesting the opportunity to prevent cancer-related inflammation.
30758933	3	15	theme	cells	987:991	arg1	proliferation					882:894	the proliferation	878:894	the proliferation	878:894	In our study, we have developed an in vitro model to evaluate the efficacy of 2D bP material with and without near-infrared light irradiation treatment, and we have demonstrated that the presence of 2D bP without treatment inhibits the metabolic activity of osteosarcoma cells (SAOS-2) while inducing both the proliferation and the osteogenic differentiation of human preosteoblast cells (HOb) and mesenchymal stem cells.
30758933	3	15	theme	cells	987:991	arg1	differentiation					915:929	the osteogenic differentiation	900:929	the osteogenic differentiation of human preosteoblast cells (HOb) and mesenchymal stem cells	900:991	In our study, we have developed an in vitro model to evaluate the efficacy of 2D bP material with and without near-infrared light irradiation treatment, and we have demonstrated that the presence of 2D bP without treatment inhibits the metabolic activity of osteosarcoma cells (SAOS-2) while inducing both the proliferation and the osteogenic differentiation of human preosteoblast cells (HOb) and mesenchymal stem cells.
30758933	6	16	theme	future	1487:1492	arg1	medicine					1507:1514	future regenerative medicine	1487:1514	future regenerative medicine	1487:1514	Finally, we have demonstrated that 2D bP represents a promising candidate for future regenerative medicine and anticancer applications.
30758933	1	17	theme	osteosarcoma	200:211	arg1	disease					213:219	osteosarcoma disease	200:219	osteosarcoma disease	200:219	Nowadays chemotherapy is the main treatment for osteosarcoma disease, even if limited by the lack of selectivity between healthy and cancer cells during the inhibition of cell division.
30758933	0	18	theme	Inflammation	127:138	arg1	Inhibition					140:149	Cancer-Related Inflammation Inhibition	112:149	Cancer-Related Inflammation Inhibition	112:149	Exfoliated Black Phosphorus Promotes in Vitro Bone Regeneration and Suppresses Osteosarcoma Progression through Cancer-Related Inflammation Inhibition.
30758933	3	19	theme	light	696:700	arg1	irradiation					702:712	near-infrared light irradiation	682:712	near-infrared light irradiation treatment	682:722	In our study, we have developed an in vitro model to evaluate the efficacy of 2D bP material with and without near-infrared light irradiation treatment, and we have demonstrated that the presence of 2D bP without treatment inhibits the metabolic activity of osteosarcoma cells (SAOS-2) while inducing both the proliferation and the osteogenic differentiation of human preosteoblast cells (HOb) and mesenchymal stem cells.
30758933	3	20	theme	metabolic	808:816	arg1	activity					818:825	the metabolic activity	804:825	the metabolic activity of osteosarcoma cells (SAOS-2)	804:856	In our study, we have developed an in vitro model to evaluate the efficacy of 2D bP material with and without near-infrared light irradiation treatment, and we have demonstrated that the presence of 2D bP without treatment inhibits the metabolic activity of osteosarcoma cells (SAOS-2) while inducing both the proliferation and the osteogenic differentiation of human preosteoblast cells (HOb) and mesenchymal stem cells.
30758933	0	21	theme	Black	11:15	arg1	Phosphorus					17:26	Exfoliated Black Phosphorus	0:26	Exfoliated Black Phosphorus	0:26	Exfoliated Black Phosphorus Promotes in Vitro Bone Regeneration and Suppresses Osteosarcoma Progression through Cancer-Related Inflammation Inhibition.
30758933	2	22	theme	2D	485:486	arg1	bP					488:489	2D bP	485:489	2D bP	485:489	Herein, we propose the use of few-layer two-dimensional black phosphorous (2D bP) as an alternative tool for osteosarcoma treatment and report how 2D bP can stimulate newly forming bone tissue generation after osteosarcoma resection.
30758933	4	23	theme	related	1142:1148	arg1	response					1133:1140	inflammatory response	1120:1140	inflammatory response related to cancer	1120:1158	Furthermore, we also propose an in vitro coculture model (SAOS-2 and HOb cell lines) in order to study the effect of 2D bP on inflammatory response related to cancer.
30758933	5	24	theme	proinflammatory	1278:1292	arg1	mediators					1294:1302	proinflammatory mediators	1278:1302	proinflammatory mediators	1278:1302	On this coculture model, 2D bP may increase anti-inflammatory cytokine generation (i.e., interleukin-10) and inhibit proinflammatory mediators synthesis (i.e., interleukin-6), thus suggesting the opportunity to prevent cancer-related inflammation.
30758933	2	25	theme	phosphorous	400:410	arg1	use					361:363	the use	357:363	the use of few-layer two-dimensional black phosphorous (2D bP) as an alternative tool for osteosarcoma treatment	357:468	Herein, we propose the use of few-layer two-dimensional black phosphorous (2D bP) as an alternative tool for osteosarcoma treatment and report how 2D bP can stimulate newly forming bone tissue generation after osteosarcoma resection.
30758933	0	26	theme	Exfoliated	0:9	arg1	Phosphorus					17:26	Exfoliated Black Phosphorus	0:26	Exfoliated Black Phosphorus	0:26	Exfoliated Black Phosphorus Promotes in Vitro Bone Regeneration and Suppresses Osteosarcoma Progression through Cancer-Related Inflammation Inhibition.
30758933	4	27	theme	cell	1067:1070	arg1	lines					1072:1076	HOb cell lines	1063:1076	HOb cell lines	1063:1076	Furthermore, we also propose an in vitro coculture model (SAOS-2 and HOb cell lines) in order to study the effect of 2D bP on inflammatory response related to cancer.
30758933	6	28	theme	anticancer	1520:1529	arg1	applications					1531:1542	anticancer applications	1520:1542	anticancer applications	1520:1542	Finally, we have demonstrated that 2D bP represents a promising candidate for future regenerative medicine and anticancer applications.
30758933	4	29	dep	model	1045:1049	arg1	lines					1072:1076	HOb cell lines	1063:1076	HOb cell lines	1063:1076	Furthermore, we also propose an in vitro coculture model (SAOS-2 and HOb cell lines) in order to study the effect of 2D bP on inflammatory response related to cancer.
30758933	4	29	dep	model	1045:1049	arg1	SAOS-2					1052:1057	SAOS-2	1052:1057	SAOS-2	1052:1057	Furthermore, we also propose an in vitro coculture model (SAOS-2 and HOb cell lines) in order to study the effect of 2D bP on inflammatory response related to cancer.
30758933	4	30	theme	HOb	1063:1065	arg1	lines					1072:1076	HOb cell lines	1063:1076	HOb cell lines	1063:1076	Furthermore, we also propose an in vitro coculture model (SAOS-2 and HOb cell lines) in order to study the effect of 2D bP on inflammatory response related to cancer.
30758933	2	31	theme	alternative	426:436	arg1	tool					438:441	an alternative tool	423:441	an alternative tool for osteosarcoma treatment	423:468	Herein, we propose the use of few-layer two-dimensional black phosphorous (2D bP) as an alternative tool for osteosarcoma treatment and report how 2D bP can stimulate newly forming bone tissue generation after osteosarcoma resection.
30758933	2	32	theme	osteosarcoma	548:559	arg1	resection					561:569	osteosarcoma resection	548:569	osteosarcoma resection	548:569	Herein, we propose the use of few-layer two-dimensional black phosphorous (2D bP) as an alternative tool for osteosarcoma treatment and report how 2D bP can stimulate newly forming bone tissue generation after osteosarcoma resection.
30758933	5	33	dep	increase	1196:1203	arg1	interleukin-10					1250:1263	interleukin-10	1250:1263	interleukin-10	1250:1263	On this coculture model, 2D bP may increase anti-inflammatory cytokine generation (i.e., interleukin-10) and inhibit proinflammatory mediators synthesis (i.e., interleukin-6), thus suggesting the opportunity to prevent cancer-related inflammation.
30758933	3	34	dep	in	607:608	arg1	vitro					610:614	vitro	610:614	vitro	610:614	In our study, we have developed an in vitro model to evaluate the efficacy of 2D bP material with and without near-infrared light irradiation treatment, and we have demonstrated that the presence of 2D bP without treatment inhibits the metabolic activity of osteosarcoma cells (SAOS-2) while inducing both the proliferation and the osteogenic differentiation of human preosteoblast cells (HOb) and mesenchymal stem cells.
30758933	3	35	theme	osteogenic	904:913	arg1	differentiation					915:929	the osteogenic differentiation	900:929	the osteogenic differentiation of human preosteoblast cells (HOb) and mesenchymal stem cells	900:991	In our study, we have developed an in vitro model to evaluate the efficacy of 2D bP material with and without near-infrared light irradiation treatment, and we have demonstrated that the presence of 2D bP without treatment inhibits the metabolic activity of osteosarcoma cells (SAOS-2) while inducing both the proliferation and the osteogenic differentiation of human preosteoblast cells (HOb) and mesenchymal stem cells.
30758933	0	36	theme	in	37:38	arg1	Regeneration					51:62	in Vitro Bone Regeneration	37:62	in Vitro Bone Regeneration	37:62	Exfoliated Black Phosphorus Promotes in Vitro Bone Regeneration and Suppresses Osteosarcoma Progression through Cancer-Related Inflammation Inhibition.
30758933	1	37	theme	cell	323:326	arg1	division					328:335	cell division	323:335	cell division	323:335	Nowadays chemotherapy is the main treatment for osteosarcoma disease, even if limited by the lack of selectivity between healthy and cancer cells during the inhibition of cell division.
30758933	5	38	theme	coculture	1169:1177	arg1	model					1179:1183	this coculture model	1164:1183	this coculture model	1164:1183	On this coculture model, 2D bP may increase anti-inflammatory cytokine generation (i.e., interleukin-10) and inhibit proinflammatory mediators synthesis (i.e., interleukin-6), thus suggesting the opportunity to prevent cancer-related inflammation.
30758933	3	39	theme	cells	954:958	arg1	proliferation					882:894	the proliferation	878:894	the proliferation	878:894	In our study, we have developed an in vitro model to evaluate the efficacy of 2D bP material with and without near-infrared light irradiation treatment, and we have demonstrated that the presence of 2D bP without treatment inhibits the metabolic activity of osteosarcoma cells (SAOS-2) while inducing both the proliferation and the osteogenic differentiation of human preosteoblast cells (HOb) and mesenchymal stem cells.
30758933	3	39	theme	cells	954:958	arg1	differentiation					915:929	the osteogenic differentiation	900:929	the osteogenic differentiation of human preosteoblast cells (HOb) and mesenchymal stem cells	900:991	In our study, we have developed an in vitro model to evaluate the efficacy of 2D bP material with and without near-infrared light irradiation treatment, and we have demonstrated that the presence of 2D bP without treatment inhibits the metabolic activity of osteosarcoma cells (SAOS-2) while inducing both the proliferation and the osteogenic differentiation of human preosteoblast cells (HOb) and mesenchymal stem cells.
30758933	2	40	theme	tissue	524:529	arg1	generation					531:540	newly forming bone tissue generation	505:540	newly forming bone tissue generation	505:540	Herein, we propose the use of few-layer two-dimensional black phosphorous (2D bP) as an alternative tool for osteosarcoma treatment and report how 2D bP can stimulate newly forming bone tissue generation after osteosarcoma resection.
30758933	6	41	theme	2D	1444:1445	arg1	bP					1447:1448	2D bP	1444:1448	2D bP	1444:1448	Finally, we have demonstrated that 2D bP represents a promising candidate for future regenerative medicine and anticancer applications.
30758933	4	42	theme	bP	1114:1115	arg1	effect					1101:1106	the effect	1097:1106	the effect of 2D bP on inflammatory response related to cancer	1097:1158	Furthermore, we also propose an in vitro coculture model (SAOS-2 and HOb cell lines) in order to study the effect of 2D bP on inflammatory response related to cancer.
30758933	0	43	theme	Bone	46:49	arg1	Regeneration					51:62	in Vitro Bone Regeneration	37:62	in Vitro Bone Regeneration	37:62	Exfoliated Black Phosphorus Promotes in Vitro Bone Regeneration and Suppresses Osteosarcoma Progression through Cancer-Related Inflammation Inhibition.
30758933	6	44	theme	promising	1463:1471	arg1	candidate					1473:1481	a promising candidate	1461:1481	a promising candidate for future regenerative medicine and anticancer applications	1461:1542	Finally, we have demonstrated that 2D bP represents a promising candidate for future regenerative medicine and anticancer applications.
30758933	3	45	theme	bP	653:654	arg1	material					656:663	2D bP material	650:663	2D bP material	650:663	In our study, we have developed an in vitro model to evaluate the efficacy of 2D bP material with and without near-infrared light irradiation treatment, and we have demonstrated that the presence of 2D bP without treatment inhibits the metabolic activity of osteosarcoma cells (SAOS-2) while inducing both the proliferation and the osteogenic differentiation of human preosteoblast cells (HOb) and mesenchymal stem cells.
30758933	0	46	dep	in	37:38	arg1	Vitro					40:44	Vitro	40:44	Vitro	40:44	Exfoliated Black Phosphorus Promotes in Vitro Bone Regeneration and Suppresses Osteosarcoma Progression through Cancer-Related Inflammation Inhibition.
30758933	3	47	theme	human	934:938	arg1	cells					954:958	human preosteoblast cells	934:958	human preosteoblast cells (HOb)	934:964	In our study, we have developed an in vitro model to evaluate the efficacy of 2D bP material with and without near-infrared light irradiation treatment, and we have demonstrated that the presence of 2D bP without treatment inhibits the metabolic activity of osteosarcoma cells (SAOS-2) while inducing both the proliferation and the osteogenic differentiation of human preosteoblast cells (HOb) and mesenchymal stem cells.
30758933	3	47	theme	human	934:938	arg1	HOb					961:963	HOb	961:963	HOb	961:963	In our study, we have developed an in vitro model to evaluate the efficacy of 2D bP material with and without near-infrared light irradiation treatment, and we have demonstrated that the presence of 2D bP without treatment inhibits the metabolic activity of osteosarcoma cells (SAOS-2) while inducing both the proliferation and the osteogenic differentiation of human preosteoblast cells (HOb) and mesenchymal stem cells.
30758933	1	48	theme	Nowadays	152:159	arg1	chemotherapy					161:172	Nowadays chemotherapy	152:172	Nowadays chemotherapy	152:172	Nowadays chemotherapy is the main treatment for osteosarcoma disease, even if limited by the lack of selectivity between healthy and cancer cells during the inhibition of cell division.
30758933	1	48	theme	Nowadays	152:159	arg1	treatment					186:194	the main treatment	177:194	the main treatment for osteosarcoma disease	177:219	Nowadays chemotherapy is the main treatment for osteosarcoma disease, even if limited by the lack of selectivity between healthy and cancer cells during the inhibition of cell division.
30758933	3	49	theme	cells	843:847	arg1	activity					818:825	the metabolic activity	804:825	the metabolic activity of osteosarcoma cells (SAOS-2)	804:856	In our study, we have developed an in vitro model to evaluate the efficacy of 2D bP material with and without near-infrared light irradiation treatment, and we have demonstrated that the presence of 2D bP without treatment inhibits the metabolic activity of osteosarcoma cells (SAOS-2) while inducing both the proliferation and the osteogenic differentiation of human preosteoblast cells (HOb) and mesenchymal stem cells.
30758933	3	50	theme	in	607:608	arg1	model					616:620	an in vitro model	604:620	an in vitro model to evaluate the efficacy of 2D bP material with and without near-infrared light irradiation treatment	604:722	In our study, we have developed an in vitro model to evaluate the efficacy of 2D bP material with and without near-infrared light irradiation treatment, and we have demonstrated that the presence of 2D bP without treatment inhibits the metabolic activity of osteosarcoma cells (SAOS-2) while inducing both the proliferation and the osteogenic differentiation of human preosteoblast cells (HOb) and mesenchymal stem cells.
30758933	3	51	theme	material	656:663	arg1	efficacy					638:645	the efficacy	634:645	the efficacy of 2D bP material with and without near-infrared light irradiation treatment	634:722	In our study, we have developed an in vitro model to evaluate the efficacy of 2D bP material with and without near-infrared light irradiation treatment, and we have demonstrated that the presence of 2D bP without treatment inhibits the metabolic activity of osteosarcoma cells (SAOS-2) while inducing both the proliferation and the osteogenic differentiation of human preosteoblast cells (HOb) and mesenchymal stem cells.
30758933	3	52	theme	preosteoblast	940:952	arg1	cells					954:958	human preosteoblast cells	934:958	human preosteoblast cells (HOb)	934:964	In our study, we have developed an in vitro model to evaluate the efficacy of 2D bP material with and without near-infrared light irradiation treatment, and we have demonstrated that the presence of 2D bP without treatment inhibits the metabolic activity of osteosarcoma cells (SAOS-2) while inducing both the proliferation and the osteogenic differentiation of human preosteoblast cells (HOb) and mesenchymal stem cells.
30758933	3	52	theme	preosteoblast	940:952	arg1	HOb					961:963	HOb	961:963	HOb	961:963	In our study, we have developed an in vitro model to evaluate the efficacy of 2D bP material with and without near-infrared light irradiation treatment, and we have demonstrated that the presence of 2D bP without treatment inhibits the metabolic activity of osteosarcoma cells (SAOS-2) while inducing both the proliferation and the osteogenic differentiation of human preosteoblast cells (HOb) and mesenchymal stem cells.
30758933	5	53	theme	anti-inflammatory	1205:1221	arg1	generation					1232:1241	anti-inflammatory cytokine generation	1205:1241	anti-inflammatory cytokine generation	1205:1241	On this coculture model, 2D bP may increase anti-inflammatory cytokine generation (i.e., interleukin-10) and inhibit proinflammatory mediators synthesis (i.e., interleukin-6), thus suggesting the opportunity to prevent cancer-related inflammation.
30758933	4	54	theme	inflammatory	1120:1131	arg1	response					1133:1140	inflammatory response	1120:1140	inflammatory response related to cancer	1120:1158	Furthermore, we also propose an in vitro coculture model (SAOS-2 and HOb cell lines) in order to study the effect of 2D bP on inflammatory response related to cancer.
30758933	5	55	dep	interleukin-10	1250:1263	arg1	i.e.					1244:1247	i.e.	1244:1247	i.e.	1244:1247	On this coculture model, 2D bP may increase anti-inflammatory cytokine generation (i.e., interleukin-10) and inhibit proinflammatory mediators synthesis (i.e., interleukin-6), thus suggesting the opportunity to prevent cancer-related inflammation.
30758933	1	56	theme	main	181:184	arg1	chemotherapy					161:172	Nowadays chemotherapy	152:172	Nowadays chemotherapy	152:172	Nowadays chemotherapy is the main treatment for osteosarcoma disease, even if limited by the lack of selectivity between healthy and cancer cells during the inhibition of cell division.
30758933	1	56	theme	main	181:184	arg1	treatment					186:194	the main treatment	177:194	the main treatment for osteosarcoma disease	177:219	Nowadays chemotherapy is the main treatment for osteosarcoma disease, even if limited by the lack of selectivity between healthy and cancer cells during the inhibition of cell division.
30758933	5	57	theme	cytokine	1223:1230	arg1	generation					1232:1241	anti-inflammatory cytokine generation	1205:1241	anti-inflammatory cytokine generation	1205:1241	On this coculture model, 2D bP may increase anti-inflammatory cytokine generation (i.e., interleukin-10) and inhibit proinflammatory mediators synthesis (i.e., interleukin-6), thus suggesting the opportunity to prevent cancer-related inflammation.
30758933	3	58	theme	2D	650:651	arg1	bP					653:654	bP	653:654	bP	653:654	In our study, we have developed an in vitro model to evaluate the efficacy of 2D bP material with and without near-infrared light irradiation treatment, and we have demonstrated that the presence of 2D bP without treatment inhibits the metabolic activity of osteosarcoma cells (SAOS-2) while inducing both the proliferation and the osteogenic differentiation of human preosteoblast cells (HOb) and mesenchymal stem cells.
30758933	4	59	from	effect	1101:1106	arg1	response					1133:1140	inflammatory response	1120:1140	inflammatory response related to cancer	1120:1158	Furthermore, we also propose an in vitro coculture model (SAOS-2 and HOb cell lines) in order to study the effect of 2D bP on inflammatory response related to cancer.
30758933	3	60	theme	mesenchymal	970:980	arg1	cells					987:991	mesenchymal stem cells	970:991	mesenchymal stem cells	970:991	In our study, we have developed an in vitro model to evaluate the efficacy of 2D bP material with and without near-infrared light irradiation treatment, and we have demonstrated that the presence of 2D bP without treatment inhibits the metabolic activity of osteosarcoma cells (SAOS-2) while inducing both the proliferation and the osteogenic differentiation of human preosteoblast cells (HOb) and mesenchymal stem cells.
30758933	3	61	theme	osteosarcoma	830:841	arg1	cells					843:847	osteosarcoma cells	830:847	osteosarcoma cells (SAOS-2)	830:856	In our study, we have developed an in vitro model to evaluate the efficacy of 2D bP material with and without near-infrared light irradiation treatment, and we have demonstrated that the presence of 2D bP without treatment inhibits the metabolic activity of osteosarcoma cells (SAOS-2) while inducing both the proliferation and the osteogenic differentiation of human preosteoblast cells (HOb) and mesenchymal stem cells.
30758933	3	61	theme	osteosarcoma	830:841	arg1	SAOS-2					850:855	SAOS-2	850:855	SAOS-2	850:855	In our study, we have developed an in vitro model to evaluate the efficacy of 2D bP material with and without near-infrared light irradiation treatment, and we have demonstrated that the presence of 2D bP without treatment inhibits the metabolic activity of osteosarcoma cells (SAOS-2) while inducing both the proliferation and the osteogenic differentiation of human preosteoblast cells (HOb) and mesenchymal stem cells.
30758933	1	62	theme	selectivity	253:263	arg1	lack					245:248	the lack	241:248	the lack of selectivity between healthy and cancer cells	241:296	Nowadays chemotherapy is the main treatment for osteosarcoma disease, even if limited by the lack of selectivity between healthy and cancer cells during the inhibition of cell division.
30758933	3	63	theme	bP	774:775	arg1	presence					759:766	the presence	755:766	the presence of 2D bP without treatment	755:793	In our study, we have developed an in vitro model to evaluate the efficacy of 2D bP material with and without near-infrared light irradiation treatment, and we have demonstrated that the presence of 2D bP without treatment inhibits the metabolic activity of osteosarcoma cells (SAOS-2) while inducing both the proliferation and the osteogenic differentiation of human preosteoblast cells (HOb) and mesenchymal stem cells.
30758933	2	64	theme	osteosarcoma	447:458	arg1	treatment					460:468	osteosarcoma treatment	447:468	osteosarcoma treatment	447:468	Herein, we propose the use of few-layer two-dimensional black phosphorous (2D bP) as an alternative tool for osteosarcoma treatment and report how 2D bP can stimulate newly forming bone tissue generation after osteosarcoma resection.
30758933	4	65	theme	in	1026:1027	arg1	model					1045:1049	an in vitro coculture model	1023:1049	an in vitro coculture model (SAOS-2 and HOb cell lines) in order to study the effect of 2D bP on inflammatory response related to cancer	1023:1158	Furthermore, we also propose an in vitro coculture model (SAOS-2 and HOb cell lines) in order to study the effect of 2D bP on inflammatory response related to cancer.
30758933	1	66	theme	division	328:335	arg1	inhibition					309:318	the inhibition	305:318	the inhibition of cell division	305:335	Nowadays chemotherapy is the main treatment for osteosarcoma disease, even if limited by the lack of selectivity between healthy and cancer cells during the inhibition of cell division.
31858044	10	0	theme	polysaccharides	1224:1238	arg1	composition					1175:1185	monosaccharide composition	1160:1185	monosaccharide composition	1160:1185	The molecular weight, monosaccharide composition, and antioxidative activities of the polysaccharides were analyzed.
31858044	10	0	theme	polysaccharides	1224:1238	arg1	activities					1206:1215	antioxidative activities	1192:1215	antioxidative activities	1192:1215	The molecular weight, monosaccharide composition, and antioxidative activities of the polysaccharides were analyzed.
31858044	10	0	theme	polysaccharides	1224:1238	arg1	weight					1152:1157	The molecular weight	1138:1157	The molecular weight	1138:1157	The molecular weight, monosaccharide composition, and antioxidative activities of the polysaccharides were analyzed.
31858044	0	1	from	Dendrobium	34:43	arg1	Extraction					0:9	Extraction	0:9	Extraction of Polysaccharide from Dendrobium	0:43	Extraction of Polysaccharide from Dendrobium nobile Lindl.
31858044	9	2	theme	ultrasound	1042:1051	arg1	extraction					1053:1062	ultrasound extraction	1042:1062	ultrasound extraction	1042:1062	The polysaccharides obtained from either the stirring extraction, refluxing extraction, ultrasound extraction, or SWE methods were compared, and the extraction processes were modeled.
31858044	8	3	theme	polysaccharide	854:867	arg1	yield					869:873	The maximum predicted polysaccharide yield	832:873	The maximum predicted polysaccharide yield	832:873	The maximum predicted polysaccharide yield was 20.67%, which corresponded well with the experiential extraction (21.88%).
31858044	8	3	theme	polysaccharide	854:867	arg1	%					884:884	20.67%	879:884	20.67%	879:884	The maximum predicted polysaccharide yield was 20.67%, which corresponded well with the experiential extraction (21.88%).
31858044	8	4	theme	predicted	844:852	arg1	yield					869:873	The maximum predicted polysaccharide yield	832:873	The maximum predicted polysaccharide yield	832:873	The maximum predicted polysaccharide yield was 20.67%, which corresponded well with the experiential extraction (21.88%).
31858044	8	4	theme	predicted	844:852	arg1	%					884:884	20.67%	879:884	20.67%	879:884	The maximum predicted polysaccharide yield was 20.67%, which corresponded well with the experiential extraction (21.88%).
31858044	10	5	theme	antioxidative	1192:1204	arg1	activities					1206:1215	antioxidative activities	1192:1215	antioxidative activities	1192:1215	The molecular weight, monosaccharide composition, and antioxidative activities of the polysaccharides were analyzed.
31858044	3	6	theme	Dendrobium	362:371	arg1	plants					373:378	Dendrobium plants	362:378	Dendrobium plants	362:378	Polysaccharides as one of the main ingredients in Dendrobium plants showed obvious biological activity.
31858044	1	7	theme	Subcritical	62:72	arg1	Extraction					80:89	Subcritical Water Extraction	62:89	Subcritical Water Extraction	62:89	by Subcritical Water Extraction.
31858044	3	8	theme	main	342:345	arg1	ingredients					347:357	the main ingredients	338:357	the main ingredients in Dendrobium plants	338:378	Polysaccharides as one of the main ingredients in Dendrobium plants showed obvious biological activity.
31858044	1	9	theme	Water	74:78	arg1	Extraction					80:89	Subcritical Water Extraction	62:89	Subcritical Water Extraction	62:89	by Subcritical Water Extraction.
31858044	9	10	theme	SWE	1068:1070	arg1	methods					1072:1078	either the stirring extraction, refluxing extraction, ultrasound extraction, or SWE methods	988:1078	methods	1072:1078	The polysaccharides obtained from either the stirring extraction, refluxing extraction, ultrasound extraction, or SWE methods were compared, and the extraction processes were modeled.
31858044	6	11	theme	response	519:526	arg1	methodology					536:546	The response surface methodology	515:546	The response surface methodology (RSM)	515:552	The response surface methodology (RSM) was combined with a Box-Behnken design to evaluate the influence that the three independent variables had on the response.
31858044	6	11	theme	response	519:526	arg1	RSM					549:551	RSM	549:551	RSM	549:551	The response surface methodology (RSM) was combined with a Box-Behnken design to evaluate the influence that the three independent variables had on the response.
31858044	2	12	theme	compressed	136:145	arg1	solvent					169:175	an effective solvent	156:175	an effective solvent for both polar and nonpolar compounds	156:213	Subcritical water extraction (SWE) uses hot compressed water as an effective solvent for both polar and nonpolar compounds and has been developed as an environmentally benign extraction technology for natural materials.
31858044	2	12	theme	compressed	136:145	arg1	water					147:151	hot compressed water	132:151	hot compressed water	132:151	Subcritical water extraction (SWE) uses hot compressed water as an effective solvent for both polar and nonpolar compounds and has been developed as an environmentally benign extraction technology for natural materials.
31858044	8	13	dep	%	884:884	arg1	%					950:950	21.88%	945:950	21.88%	945:950	The maximum predicted polysaccharide yield was 20.67%, which corresponded well with the experiential extraction (21.88%).
31858044	2	14	theme	benign	260:265	arg1	technology					278:287	an environmentally benign extraction technology	241:287	an environmentally benign extraction technology for natural materials	241:309	Subcritical water extraction (SWE) uses hot compressed water as an effective solvent for both polar and nonpolar compounds and has been developed as an environmentally benign extraction technology for natural materials.
31858044	2	14	theme	benign	260:265	arg1	extraction					110:119	Subcritical water extraction	92:119	Subcritical water extraction (SWE)	92:125	Subcritical water extraction (SWE) uses hot compressed water as an effective solvent for both polar and nonpolar compounds and has been developed as an environmentally benign extraction technology for natural materials.
31858044	6	15	theme	surface	528:534	arg1	methodology					536:546	The response surface methodology	515:546	The response surface methodology (RSM)	515:552	The response surface methodology (RSM) was combined with a Box-Behnken design to evaluate the influence that the three independent variables had on the response.
31858044	6	15	theme	surface	528:534	arg1	RSM					549:551	RSM	549:551	RSM	549:551	The response surface methodology (RSM) was combined with a Box-Behnken design to evaluate the influence that the three independent variables had on the response.
31858044	3	16	theme	obvious	387:393	arg1	activity					406:413	obvious biological activity	387:413	obvious biological activity	387:413	Polysaccharides as one of the main ingredients in Dendrobium plants showed obvious biological activity.
31858044	2	17	theme	hot	132:134	arg1	solvent					169:175	an effective solvent	156:175	an effective solvent for both polar and nonpolar compounds	156:213	Subcritical water extraction (SWE) uses hot compressed water as an effective solvent for both polar and nonpolar compounds and has been developed as an environmentally benign extraction technology for natural materials.
31858044	2	17	theme	hot	132:134	arg1	water					147:151	hot compressed water	132:151	hot compressed water	132:151	Subcritical water extraction (SWE) uses hot compressed water as an effective solvent for both polar and nonpolar compounds and has been developed as an environmentally benign extraction technology for natural materials.
31858044	3	18	theme	biological	395:404	arg1	activity					406:413	obvious biological activity	387:413	obvious biological activity	387:413	Polysaccharides as one of the main ingredients in Dendrobium plants showed obvious biological activity.
31858044	7	19	theme	1.12	802:805	arg1	MPa					807:809	MPa	807:809	MPa	807:809	The optimal extraction conditions (determined via RSM) were 129.83 °C extraction temperature, 16.71 min extraction time, and 1.12 MPa extraction pressure.
31858044	10	20	theme	molecular	1142:1150	arg1	weight					1152:1157	The molecular weight	1138:1157	The molecular weight	1138:1157	The molecular weight, monosaccharide composition, and antioxidative activities of the polysaccharides were analyzed.
31858044	0	21	theme	Polysaccharide	14:27	arg1	Extraction					0:9	Extraction	0:9	Extraction of Polysaccharide from Dendrobium	0:43	Extraction of Polysaccharide from Dendrobium nobile Lindl.
31858044	2	22	theme	extraction	267:276	arg1	technology					278:287	an environmentally benign extraction technology	241:287	an environmentally benign extraction technology for natural materials	241:309	Subcritical water extraction (SWE) uses hot compressed water as an effective solvent for both polar and nonpolar compounds and has been developed as an environmentally benign extraction technology for natural materials.
31858044	2	22	theme	extraction	267:276	arg1	extraction					110:119	Subcritical water extraction	92:119	Subcritical water extraction (SWE)	92:125	Subcritical water extraction (SWE) uses hot compressed water as an effective solvent for both polar and nonpolar compounds and has been developed as an environmentally benign extraction technology for natural materials.
31858044	2	23	theme	natural	293:299	arg1	materials					301:309	natural materials	293:309	natural materials	293:309	Subcritical water extraction (SWE) uses hot compressed water as an effective solvent for both polar and nonpolar compounds and has been developed as an environmentally benign extraction technology for natural materials.
31858044	7	24	theme	MPa	807:809	arg1	pressure					822:829	1.12 MPa extraction pressure	802:829	1.12 MPa extraction pressure	802:829	The optimal extraction conditions (determined via RSM) were 129.83 °C extraction temperature, 16.71 min extraction time, and 1.12 MPa extraction pressure.
31858044	3	25	from	ingredients	347:357	arg1	plants					373:378	Dendrobium plants	362:378	Dendrobium plants	362:378	Polysaccharides as one of the main ingredients in Dendrobium plants showed obvious biological activity.
31858044	9	26	theme	extraction	1053:1062	arg1	methods					1072:1078	either the stirring extraction, refluxing extraction, ultrasound extraction, or SWE methods	988:1078	methods	1072:1078	The polysaccharides obtained from either the stirring extraction, refluxing extraction, ultrasound extraction, or SWE methods were compared, and the extraction processes were modeled.
31858044	7	27	theme	extraction	811:820	arg1	pressure					822:829	1.12 MPa extraction pressure	802:829	1.12 MPa extraction pressure	802:829	The optimal extraction conditions (determined via RSM) were 129.83 °C extraction temperature, 16.71 min extraction time, and 1.12 MPa extraction pressure.
31858044	6	28	theme	Box-Behnken	574:584	arg1	design					586:591	a Box-Behnken design	572:591	a Box-Behnken design to evaluate the influence that the three independent variables had on the response	572:674	The response surface methodology (RSM) was combined with a Box-Behnken design to evaluate the influence that the three independent variables had on the response.
31858044	7	29	theme	min	777:779	arg1	time					792:795	16.71 min extraction time	771:795	16.71 min extraction time	771:795	The optimal extraction conditions (determined via RSM) were 129.83 °C extraction temperature, 16.71 min extraction time, and 1.12 MPa extraction pressure.
31858044	2	30	theme	effective	159:167	arg1	solvent					169:175	an effective solvent	156:175	an effective solvent for both polar and nonpolar compounds	156:213	Subcritical water extraction (SWE) uses hot compressed water as an effective solvent for both polar and nonpolar compounds and has been developed as an environmentally benign extraction technology for natural materials.
31858044	2	30	theme	effective	159:167	arg1	water					147:151	hot compressed water	132:151	hot compressed water	132:151	Subcritical water extraction (SWE) uses hot compressed water as an effective solvent for both polar and nonpolar compounds and has been developed as an environmentally benign extraction technology for natural materials.
31858044	9	31	theme	extraction	1103:1112	arg1	processes					1114:1122	the extraction processes	1099:1122	the extraction processes	1099:1122	The polysaccharides obtained from either the stirring extraction, refluxing extraction, ultrasound extraction, or SWE methods were compared, and the extraction processes were modeled.
31858044	3	32	theme	ingredients	347:357	arg1	ingredients					347:357	the main ingredients	338:357	the main ingredients in Dendrobium plants	338:378	Polysaccharides as one of the main ingredients in Dendrobium plants showed obvious biological activity.
31858044	3	32	theme	ingredients	347:357	arg1	one					331:333	one	331:333	one	331:333	Polysaccharides as one of the main ingredients in Dendrobium plants showed obvious biological activity.
31858044	2	33	theme	water	104:108	arg1	technology					278:287	an environmentally benign extraction technology	241:287	an environmentally benign extraction technology for natural materials	241:309	Subcritical water extraction (SWE) uses hot compressed water as an effective solvent for both polar and nonpolar compounds and has been developed as an environmentally benign extraction technology for natural materials.
31858044	2	33	theme	water	104:108	arg1	SWE					122:124	SWE	122:124	SWE	122:124	Subcritical water extraction (SWE) uses hot compressed water as an effective solvent for both polar and nonpolar compounds and has been developed as an environmentally benign extraction technology for natural materials.
31858044	2	33	theme	water	104:108	arg1	extraction					110:119	Subcritical water extraction	92:119	Subcritical water extraction (SWE)	92:125	Subcritical water extraction (SWE) uses hot compressed water as an effective solvent for both polar and nonpolar compounds and has been developed as an environmentally benign extraction technology for natural materials.
31858044	9	34	theme	stirring	999:1006	arg1	extraction					1008:1017	stirring extraction	999:1017	stirring extraction	999:1017	The polysaccharides obtained from either the stirring extraction, refluxing extraction, ultrasound extraction, or SWE methods were compared, and the extraction processes were modeled.
31858044	2	35	theme	Subcritical	92:102	arg1	technology					278:287	an environmentally benign extraction technology	241:287	an environmentally benign extraction technology for natural materials	241:309	Subcritical water extraction (SWE) uses hot compressed water as an effective solvent for both polar and nonpolar compounds and has been developed as an environmentally benign extraction technology for natural materials.
31858044	2	35	theme	Subcritical	92:102	arg1	SWE					122:124	SWE	122:124	SWE	122:124	Subcritical water extraction (SWE) uses hot compressed water as an effective solvent for both polar and nonpolar compounds and has been developed as an environmentally benign extraction technology for natural materials.
31858044	2	35	theme	Subcritical	92:102	arg1	extraction					110:119	Subcritical water extraction	92:119	Subcritical water extraction (SWE)	92:125	Subcritical water extraction (SWE) uses hot compressed water as an effective solvent for both polar and nonpolar compounds and has been developed as an environmentally benign extraction technology for natural materials.
31858044	2	36	theme	nonpolar	196:203	arg1	compounds					205:213	both polar and nonpolar compounds	181:213	both polar and nonpolar compounds	181:213	Subcritical water extraction (SWE) uses hot compressed water as an effective solvent for both polar and nonpolar compounds and has been developed as an environmentally benign extraction technology for natural materials.
31858044	7	37	theme	extraction	689:698	arg1	conditions					700:709	The optimal extraction conditions	677:709	The optimal extraction conditions (determined via RSM)	677:730	The optimal extraction conditions (determined via RSM) were 129.83 °C extraction temperature, 16.71 min extraction time, and 1.12 MPa extraction pressure.
31858044	7	37	theme	extraction	689:698	arg1	129.83					737:742	129.83	737:742	129.83	737:742	The optimal extraction conditions (determined via RSM) were 129.83 °C extraction temperature, 16.71 min extraction time, and 1.12 MPa extraction pressure.
31858044	9	38	theme	extraction	1008:1017	arg1	methods					1072:1078	either the stirring extraction, refluxing extraction, ultrasound extraction, or SWE methods	988:1078	methods	1072:1078	The polysaccharides obtained from either the stirring extraction, refluxing extraction, ultrasound extraction, or SWE methods were compared, and the extraction processes were modeled.
31858044	7	39	theme	16.71	771:775	arg1	min					777:779	min	777:779	min	777:779	The optimal extraction conditions (determined via RSM) were 129.83 °C extraction temperature, 16.71 min extraction time, and 1.12 MPa extraction pressure.
31858044	7	40	theme	optimal	681:687	arg1	conditions					700:709	The optimal extraction conditions	677:709	The optimal extraction conditions (determined via RSM)	677:730	The optimal extraction conditions (determined via RSM) were 129.83 °C extraction temperature, 16.71 min extraction time, and 1.12 MPa extraction pressure.
31858044	7	40	theme	optimal	681:687	arg1	129.83					737:742	129.83	737:742	129.83	737:742	The optimal extraction conditions (determined via RSM) were 129.83 °C extraction temperature, 16.71 min extraction time, and 1.12 MPa extraction pressure.
31858044	8	41	theme	experiential	920:931	arg1	extraction					933:942	the experiential extraction	916:942	the experiential extraction	916:942	The maximum predicted polysaccharide yield was 20.67%, which corresponded well with the experiential extraction (21.88%).
31858044	7	42	theme	extraction	747:756	arg1	temperature					758:768	extraction temperature	747:768	extraction temperature	747:768	The optimal extraction conditions (determined via RSM) were 129.83 °C extraction temperature, 16.71 min extraction time, and 1.12 MPa extraction pressure.
31858044	2	43	theme	polar	186:190	arg1	compounds					205:213	both polar and nonpolar compounds	181:213	both polar and nonpolar compounds	181:213	Subcritical water extraction (SWE) uses hot compressed water as an effective solvent for both polar and nonpolar compounds and has been developed as an environmentally benign extraction technology for natural materials.
31858044	9	44	theme	refluxing	1020:1028	arg1	extraction					1030:1039	refluxing extraction	1020:1039	refluxing extraction	1020:1039	The polysaccharides obtained from either the stirring extraction, refluxing extraction, ultrasound extraction, or SWE methods were compared, and the extraction processes were modeled.
31858044	7	45	dep	conditions	700:709	arg1	determined					712:721	determined	712:721	determined via RSM	712:729	The optimal extraction conditions (determined via RSM) were 129.83 °C extraction temperature, 16.71 min extraction time, and 1.12 MPa extraction pressure.
31858044	4	46	theme	polysaccharides	429:443	arg1	SWE					422:424	SWE	422:424	SWE of polysaccharides obtained from Dendrobium	422:468	Thus, SWE of polysaccharides obtained from Dendrobium nobile Lindl.
31858044	8	47	theme	maximum	836:842	arg1	yield					869:873	The maximum predicted polysaccharide yield	832:873	The maximum predicted polysaccharide yield	832:873	The maximum predicted polysaccharide yield was 20.67%, which corresponded well with the experiential extraction (21.88%).
31858044	8	47	theme	maximum	836:842	arg1	%					884:884	20.67%	879:884	20.67%	879:884	The maximum predicted polysaccharide yield was 20.67%, which corresponded well with the experiential extraction (21.88%).
31858044	9	48	theme	extraction	1030:1039	arg1	methods					1072:1078	either the stirring extraction, refluxing extraction, ultrasound extraction, or SWE methods	988:1078	methods	1072:1078	The polysaccharides obtained from either the stirring extraction, refluxing extraction, ultrasound extraction, or SWE methods were compared, and the extraction processes were modeled.
31858044	10	49	theme	monosaccharide	1160:1173	arg1	composition					1175:1185	monosaccharide composition	1160:1185	monosaccharide composition	1160:1185	The molecular weight, monosaccharide composition, and antioxidative activities of the polysaccharides were analyzed.
31858044	6	50	theme	independent	634:644	arg1	variables					646:654	the three independent variables	624:654	the three independent variables	624:654	The response surface methodology (RSM) was combined with a Box-Behnken design to evaluate the influence that the three independent variables had on the response.
31858044	7	51	theme	extraction	781:790	arg1	time					792:795	16.71 min extraction time	771:795	16.71 min extraction time	771:795	The optimal extraction conditions (determined via RSM) were 129.83 °C extraction temperature, 16.71 min extraction time, and 1.12 MPa extraction pressure.
30028282	9	0	from	members	1199:1205	arg1	differentiation					1153:1167	the clear differentiation	1143:1167	the clear differentiation of strain G27T from all other members of the family Bacillaceae.It is therefore considered to represent a novel species of a new genus, for which the name Thalassorhabdus alkalitolerans gen. nov., sp	1143:1367	The results of phylogenetic, chemotaxonomic and biochemical tests allowed the clear differentiation of strain G27T from all other members of the family Bacillaceae.It is therefore considered to represent a novel species of a new genus, for which the name Thalassorhabdus alkalitolerans gen. nov., sp.
30028282	6	1	theme	unidentified	889:900	arg1	lipids					902:907	five unidentified lipids	884:907	five unidentified lipids	884:907	Polar lipids included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unidentified phospholipid and five unidentified lipids.
30028282	9	2	theme	name	1319:1322	arg1	Thalassorhabdus					1324:1338	the name Thalassorhabdus	1315:1338	the name Thalassorhabdus	1315:1338	The results of phylogenetic, chemotaxonomic and biochemical tests allowed the clear differentiation of strain G27T from all other members of the family Bacillaceae.It is therefore considered to represent a novel species of a new genus, for which the name Thalassorhabdus alkalitolerans gen. nov., sp.
30028282	4	3	theme	43.4±0.6 mol	677:688	arg1	content					650:656	The DNA G+C content	638:656	The DNA G+C content of strain G27T	638:671	The DNA G+C content of strain G27T was 43.4±0.6 mol%.
30028282	4	3	theme	43.4±0.6 mol	677:688	arg1	%					689:689	43.4±0.6 mol%	677:689	43.4±0.6 mol%	677:689	The DNA G+C content of strain G27T was 43.4±0.6 mol%.
30028282	9	4	theme	gen.	1355:1358	arg1	nov.					1360:1363	gen. nov.	1355:1363	gen. nov.	1355:1363	The results of phylogenetic, chemotaxonomic and biochemical tests allowed the clear differentiation of strain G27T from all other members of the family Bacillaceae.It is therefore considered to represent a novel species of a new genus, for which the name Thalassorhabdus alkalitolerans gen. nov., sp.
30028282	9	4	theme	gen.	1355:1358	arg1	sp					1366:1367	sp	1366:1367	sp	1366:1367	The results of phylogenetic, chemotaxonomic and biochemical tests allowed the clear differentiation of strain G27T from all other members of the family Bacillaceae.It is therefore considered to represent a novel species of a new genus, for which the name Thalassorhabdus alkalitolerans gen. nov., sp.
30028282	11	5	theme	type	1393:1396	arg1	G27T					1442:1445	G27T	1442:1445	G27T (=MCC 3411T=CGMCC 1.15772T=KCTC 33941T)	1442:1485	The type strain of Thalassorhabdus alkalitolerans is G27T (=MCC 3411T=CGMCC 1.15772T=KCTC 33941T).
30028282	11	5	theme	type	1393:1396	arg1	strain					1398:1403	The type strain	1389:1403	The type strain of Thalassorhabdus alkalitolerans	1389:1437	The type strain of Thalassorhabdus alkalitolerans is G27T (=MCC 3411T=CGMCC 1.15772T=KCTC 33941T).
30028282	9	6	theme	novel	1275:1279	arg1	species					1281:1287	a novel species	1273:1287	a novel species of a new genus, for which the name Thalassorhabdus alkalitolerans gen. nov., sp	1273:1367	The results of phylogenetic, chemotaxonomic and biochemical tests allowed the clear differentiation of strain G27T from all other members of the family Bacillaceae.It is therefore considered to represent a novel species of a new genus, for which the name Thalassorhabdus alkalitolerans gen. nov., sp.
30028282	7	7	theme	menaquinone	949:959	arg1	quinone					937:943	The predominant isoprenoid quinone	910:943	The predominant isoprenoid quinone	910:943	The predominant isoprenoid quinone was menaquinone MK-7.
30028282	7	7	theme	menaquinone	949:959	arg1	MK-7					961:964	menaquinone MK-7	949:964	menaquinone MK-7	949:964	The predominant isoprenoid quinone was menaquinone MK-7.
30028282	5	8	contain	contained	720:728	arg1	peptidoglycan					706:718	The cell-wall peptidoglycan	692:718	The cell-wall peptidoglycan	692:718	The cell-wall peptidoglycan contained meso-diaminopimelic acid.
30028282	5	8	contain	contained	720:728	arg2	acid					750:753	meso-diaminopimelic acid	730:753	meso-diaminopimelic acid	730:753	The cell-wall peptidoglycan contained meso-diaminopimelic acid.
30028282	9	9	dep	family	1214:1219	arg1	considered					1249:1258	considered	1249:1258	is therefore considered to represent a novel species of a new genus, for which the name Thalassorhabdus alkalitolerans gen. nov., sp	1236:1367	The results of phylogenetic, chemotaxonomic and biochemical tests allowed the clear differentiation of strain G27T from all other members of the family Bacillaceae.It is therefore considered to represent a novel species of a new genus, for which the name Thalassorhabdus alkalitolerans gen. nov., sp.
30028282	8	10	dep	iso-C15 	1016:1023	arg1	 0					1025:1026	 0	1025:1026	 0	1025:1026	Major fatty acids (>5 %) included anteiso-C15:0, iso-C15 : 0, anteiso-C17:0, C16 : 0 and iso-C16 : 0.
30028282	8	10	dep	iso-C15 	1016:1023	arg1	iso-C16 					1056:1063	iso-C16 	1056:1063	iso-C16 	1056:1063	Major fatty acids (>5 %) included anteiso-C15:0, iso-C15 : 0, anteiso-C17:0, C16 : 0 and iso-C16 : 0.
30028282	8	10	dep	iso-C15 	1016:1023	arg1	 0					1049:1050	 0	1049:1050	 0	1049:1050	Major fatty acids (>5 %) included anteiso-C15:0, iso-C15 : 0, anteiso-C17:0, C16 : 0 and iso-C16 : 0.
30028282	8	10	dep	iso-C15 	1016:1023	arg1	 0					1065:1066	 0	1065:1066	 0	1065:1066	Major fatty acids (>5 %) included anteiso-C15:0, iso-C15 : 0, anteiso-C17:0, C16 : 0 and iso-C16 : 0.
30028282	9	11	theme	tests	1129:1133	arg1	results					1073:1079	The results	1069:1079	The results of phylogenetic, chemotaxonomic and biochemical tests	1069:1133	The results of phylogenetic, chemotaxonomic and biochemical tests allowed the clear differentiation of strain G27T from all other members of the family Bacillaceae.It is therefore considered to represent a novel species of a new genus, for which the name Thalassorhabdus alkalitolerans gen. nov., sp.
30028282	3	12	theme	Salipaludibacillus	504:521	arg1	13187T					541:546	Salipaludibacillus neizhouensis KCTC 13187T	504:546	Salipaludibacillus neizhouensis KCTC 13187T (94.7 %)	504:555	Based on 16S rRNA gene sequence analysis, strain G27T was identified as a member of the class Firmibacteria and was most closely related to Bacillus populi FJAT-45347T (94.9 % sequence similarity), Salipaludibacillus aurantiacus S9T (94.9 %), Salipaludibacillus neizhouensis KCTC 13187T (94.7 %), Alteribacillus iranensis DSM 23995T (94.6 %) and other Firmibacteria (<94.6 %).
30028282	3	12	theme	Salipaludibacillus	504:521	arg1	%					554:554	94.7 %	549:554	94.7 %	549:554	Based on 16S rRNA gene sequence analysis, strain G27T was identified as a member of the class Firmibacteria and was most closely related to Bacillus populi FJAT-45347T (94.9 % sequence similarity), Salipaludibacillus aurantiacus S9T (94.9 %), Salipaludibacillus neizhouensis KCTC 13187T (94.7 %), Alteribacillus iranensis DSM 23995T (94.6 %) and other Firmibacteria (<94.6 %).
30028282	5	13	theme	meso-diaminopimelic	730:748	arg1	acid					750:753	meso-diaminopimelic acid	730:753	meso-diaminopimelic acid	730:753	The cell-wall peptidoglycan contained meso-diaminopimelic acid.
30028282	11	14	theme	3411T=CGMCC	1453:1463	arg1	33941T					1479:1484	=MCC 3411T=CGMCC 1.15772T=KCTC 33941T	1448:1484	=MCC 3411T=CGMCC 1.15772T=KCTC 33941T	1448:1484	The type strain of Thalassorhabdus alkalitolerans is G27T (=MCC 3411T=CGMCC 1.15772T=KCTC 33941T).
30028282	11	14	theme	3411T=CGMCC	1453:1463	arg1	G27T					1442:1445	G27T	1442:1445	G27T (=MCC 3411T=CGMCC 1.15772T=KCTC 33941T)	1442:1485	The type strain of Thalassorhabdus alkalitolerans is G27T (=MCC 3411T=CGMCC 1.15772T=KCTC 33941T).
30028282	4	15	theme	G27T	668:671	arg1	content					650:656	The DNA G+C content	638:656	The DNA G+C content of strain G27T	638:671	The DNA G+C content of strain G27T was 43.4±0.6 mol%.
30028282	4	15	theme	G27T	668:671	arg1	%					689:689	43.4±0.6 mol%	677:689	43.4±0.6 mol%	677:689	The DNA G+C content of strain G27T was 43.4±0.6 mol%.
30028282	2	16	theme	rod-shaped	139:148	arg1	bacterium					188:196	A novel Gram-stain-variable, rod-shaped, non-motile and non-endospore-forming bacterium	110:196	A novel Gram-stain-variable, rod-shaped, non-motile and non-endospore-forming bacterium (strain G27T)	110:210	A novel Gram-stain-variable, rod-shaped, non-motile and non-endospore-forming bacterium (strain G27T) was isolated from near Dhuvaran, Gujarat, India.
30028282	2	16	theme	rod-shaped	139:148	arg1	G27T					206:209	strain G27T	199:209	strain G27T	199:209	A novel Gram-stain-variable, rod-shaped, non-motile and non-endospore-forming bacterium (strain G27T) was isolated from near Dhuvaran, Gujarat, India.
30028282	8	17	theme	fatty	973:977	arg1	acids					979:983	Major fatty acids	967:983	Major fatty acids (>5 %)	967:990	Major fatty acids (>5 %) included anteiso-C15:0, iso-C15 : 0, anteiso-C17:0, C16 : 0 and iso-C16 : 0.
30028282	8	17	theme	fatty	973:977	arg1	%					989:989	>5 %	986:989	>5 %	986:989	Major fatty acids (>5 %) included anteiso-C15:0, iso-C15 : 0, anteiso-C17:0, C16 : 0 and iso-C16 : 0.
30028282	1	18	theme	novel	54:58	arg1	member					72:77	a novel Bacillaceae member	52:77	a novel Bacillaceae member	52:77	nov., a novel Bacillaceae member isolated from marine sediment.
30028282	1	18	theme	novel	54:58	arg1	nov.					46:49	nov.	46:49	nov.	46:49	nov., a novel Bacillaceae member isolated from marine sediment.
30028282	9	19	theme	biochemical	1117:1127	arg1	tests					1129:1133	phylogenetic, chemotaxonomic and biochemical tests	1084:1133	phylogenetic, chemotaxonomic and biochemical tests	1084:1133	The results of phylogenetic, chemotaxonomic and biochemical tests allowed the clear differentiation of strain G27T from all other members of the family Bacillaceae.It is therefore considered to represent a novel species of a new genus, for which the name Thalassorhabdus alkalitolerans gen. nov., sp.
30028282	7	20	theme	predominant	914:924	arg1	quinone					937:943	The predominant isoprenoid quinone	910:943	The predominant isoprenoid quinone	910:943	The predominant isoprenoid quinone was menaquinone MK-7.
30028282	7	20	theme	predominant	914:924	arg1	MK-7					961:964	menaquinone MK-7	949:964	menaquinone MK-7	949:964	The predominant isoprenoid quinone was menaquinone MK-7.
30028282	1	21	theme	Bacillaceae	60:70	arg1	member					72:77	a novel Bacillaceae member	52:77	a novel Bacillaceae member	52:77	nov., a novel Bacillaceae member isolated from marine sediment.
30028282	1	21	theme	Bacillaceae	60:70	arg1	nov.					46:49	nov.	46:49	nov.	46:49	nov., a novel Bacillaceae member isolated from marine sediment.
30028282	3	22	theme	Bacillus	401:408	arg1	similarity					446:455	94.9 % sequence similarity	430:455	94.9 % sequence similarity	430:455	Based on 16S rRNA gene sequence analysis, strain G27T was identified as a member of the class Firmibacteria and was most closely related to Bacillus populi FJAT-45347T (94.9 % sequence similarity), Salipaludibacillus aurantiacus S9T (94.9 %), Salipaludibacillus neizhouensis KCTC 13187T (94.7 %), Alteribacillus iranensis DSM 23995T (94.6 %) and other Firmibacteria (<94.6 %).
30028282	3	22	theme	Bacillus	401:408	arg1	FJAT-45347T					417:427	Bacillus populi FJAT-45347T	401:427	Bacillus populi FJAT-45347T (94.9 % sequence similarity)	401:456	Based on 16S rRNA gene sequence analysis, strain G27T was identified as a member of the class Firmibacteria and was most closely related to Bacillus populi FJAT-45347T (94.9 % sequence similarity), Salipaludibacillus aurantiacus S9T (94.9 %), Salipaludibacillus neizhouensis KCTC 13187T (94.7 %), Alteribacillus iranensis DSM 23995T (94.6 %) and other Firmibacteria (<94.6 %).
30028282	9	23	theme	clear	1147:1151	arg1	differentiation					1153:1167	the clear differentiation	1143:1167	the clear differentiation of strain G27T from all other members of the family Bacillaceae.It is therefore considered to represent a novel species of a new genus, for which the name Thalassorhabdus alkalitolerans gen. nov., sp	1143:1367	The results of phylogenetic, chemotaxonomic and biochemical tests allowed the clear differentiation of strain G27T from all other members of the family Bacillaceae.It is therefore considered to represent a novel species of a new genus, for which the name Thalassorhabdus alkalitolerans gen. nov., sp.
30028282	2	24	theme	Gram-stain-variable	118:136	arg1	bacterium					188:196	A novel Gram-stain-variable, rod-shaped, non-motile and non-endospore-forming bacterium	110:196	A novel Gram-stain-variable, rod-shaped, non-motile and non-endospore-forming bacterium (strain G27T)	110:210	A novel Gram-stain-variable, rod-shaped, non-motile and non-endospore-forming bacterium (strain G27T) was isolated from near Dhuvaran, Gujarat, India.
30028282	2	24	theme	Gram-stain-variable	118:136	arg1	G27T					206:209	strain G27T	199:209	strain G27T	199:209	A novel Gram-stain-variable, rod-shaped, non-motile and non-endospore-forming bacterium (strain G27T) was isolated from near Dhuvaran, Gujarat, India.
30028282	7	25	theme	isoprenoid	926:935	arg1	quinone					937:943	The predominant isoprenoid quinone	910:943	The predominant isoprenoid quinone	910:943	The predominant isoprenoid quinone was menaquinone MK-7.
30028282	7	25	theme	isoprenoid	926:935	arg1	MK-7					961:964	menaquinone MK-7	949:964	menaquinone MK-7	949:964	The predominant isoprenoid quinone was menaquinone MK-7.
30028282	6	26	theme	unidentified	854:865	arg1	phospholipid					867:878	one unidentified phospholipid	850:878	one unidentified phospholipid	850:878	Polar lipids included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unidentified phospholipid and five unidentified lipids.
30028282	3	27	theme	class	349:353	arg1	Firmibacteria					355:367	the class Firmibacteria	345:367	the class Firmibacteria	345:367	Based on 16S rRNA gene sequence analysis, strain G27T was identified as a member of the class Firmibacteria and was most closely related to Bacillus populi FJAT-45347T (94.9 % sequence similarity), Salipaludibacillus aurantiacus S9T (94.9 %), Salipaludibacillus neizhouensis KCTC 13187T (94.7 %), Alteribacillus iranensis DSM 23995T (94.6 %) and other Firmibacteria (<94.6 %).
30028282	9	28	theme	family	1214:1219	arg1	members					1199:1205	all other members	1189:1205	all other members of the family Bacillaceae.It is therefore considered to represent a novel species of a new genus, for which the name Thalassorhabdus alkalitolerans gen. nov., sp	1189:1367	The results of phylogenetic, chemotaxonomic and biochemical tests allowed the clear differentiation of strain G27T from all other members of the family Bacillaceae.It is therefore considered to represent a novel species of a new genus, for which the name Thalassorhabdus alkalitolerans gen. nov., sp.
30028282	3	29	theme	KCTC	536:539	arg1	13187T					541:546	Salipaludibacillus neizhouensis KCTC 13187T	504:546	Salipaludibacillus neizhouensis KCTC 13187T (94.7 %)	504:555	Based on 16S rRNA gene sequence analysis, strain G27T was identified as a member of the class Firmibacteria and was most closely related to Bacillus populi FJAT-45347T (94.9 % sequence similarity), Salipaludibacillus aurantiacus S9T (94.9 %), Salipaludibacillus neizhouensis KCTC 13187T (94.7 %), Alteribacillus iranensis DSM 23995T (94.6 %) and other Firmibacteria (<94.6 %).
30028282	3	29	theme	KCTC	536:539	arg1	%					554:554	94.7 %	549:554	94.7 %	549:554	Based on 16S rRNA gene sequence analysis, strain G27T was identified as a member of the class Firmibacteria and was most closely related to Bacillus populi FJAT-45347T (94.9 % sequence similarity), Salipaludibacillus aurantiacus S9T (94.9 %), Salipaludibacillus neizhouensis KCTC 13187T (94.7 %), Alteribacillus iranensis DSM 23995T (94.6 %) and other Firmibacteria (<94.6 %).
30028282	4	30	theme	G+C	646:648	arg1	content					650:656	The DNA G+C content	638:656	The DNA G+C content of strain G27T	638:671	The DNA G+C content of strain G27T was 43.4±0.6 mol%.
30028282	4	30	theme	G+C	646:648	arg1	%					689:689	43.4±0.6 mol%	677:689	43.4±0.6 mol%	677:689	The DNA G+C content of strain G27T was 43.4±0.6 mol%.
30028282	9	31	theme	strain	1172:1177	arg1	G27T					1179:1182	strain G27T	1172:1182	strain G27T	1172:1182	The results of phylogenetic, chemotaxonomic and biochemical tests allowed the clear differentiation of strain G27T from all other members of the family Bacillaceae.It is therefore considered to represent a novel species of a new genus, for which the name Thalassorhabdus alkalitolerans gen. nov., sp.
30028282	4	32	theme	strain	661:666	arg1	G27T					668:671	strain G27T	661:671	strain G27T	661:671	The DNA G+C content of strain G27T was 43.4±0.6 mol%.
30028282	8	33	theme	Major	967:971	arg1	acids					979:983	Major fatty acids	967:983	Major fatty acids (>5 %)	967:990	Major fatty acids (>5 %) included anteiso-C15:0, iso-C15 : 0, anteiso-C17:0, C16 : 0 and iso-C16 : 0.
30028282	8	33	theme	Major	967:971	arg1	%					989:989	>5 %	986:989	>5 %	986:989	Major fatty acids (>5 %) included anteiso-C15:0, iso-C15 : 0, anteiso-C17:0, C16 : 0 and iso-C16 : 0.
30028282	0	34	theme	gen.	31:34	arg1	sp					42:43	sp	42:43	sp	42:43	Thalassorhabdus alkalitolerans gen. nov., sp.
30028282	0	34	theme	gen.	31:34	arg1	nov.					36:39	gen. nov.	31:39	gen. nov.	31:39	Thalassorhabdus alkalitolerans gen. nov., sp.
30028282	11	35	theme	=MCC	1448:1451	arg1	33941T					1479:1484	=MCC 3411T=CGMCC 1.15772T=KCTC 33941T	1448:1484	=MCC 3411T=CGMCC 1.15772T=KCTC 33941T	1448:1484	The type strain of Thalassorhabdus alkalitolerans is G27T (=MCC 3411T=CGMCC 1.15772T=KCTC 33941T).
30028282	11	35	theme	=MCC	1448:1451	arg1	G27T					1442:1445	G27T	1442:1445	G27T (=MCC 3411T=CGMCC 1.15772T=KCTC 33941T)	1442:1485	The type strain of Thalassorhabdus alkalitolerans is G27T (=MCC 3411T=CGMCC 1.15772T=KCTC 33941T).
30028282	9	36	theme	G27T	1179:1182	arg1	differentiation					1153:1167	the clear differentiation	1143:1167	the clear differentiation of strain G27T from all other members of the family Bacillaceae.It is therefore considered to represent a novel species of a new genus, for which the name Thalassorhabdus alkalitolerans gen. nov., sp	1143:1367	The results of phylogenetic, chemotaxonomic and biochemical tests allowed the clear differentiation of strain G27T from all other members of the family Bacillaceae.It is therefore considered to represent a novel species of a new genus, for which the name Thalassorhabdus alkalitolerans gen. nov., sp.
30028282	1	37	theme	marine	93:98	arg1	sediment					100:107	marine sediment	93:107	marine sediment	93:107	nov., a novel Bacillaceae member isolated from marine sediment.
30028282	9	38	theme	new	1294:1296	arg1	genus					1298:1302	a new genus	1292:1302	a new genus	1292:1302	The results of phylogenetic, chemotaxonomic and biochemical tests allowed the clear differentiation of strain G27T from all other members of the family Bacillaceae.It is therefore considered to represent a novel species of a new genus, for which the name Thalassorhabdus alkalitolerans gen. nov., sp.
30028282	2	39	theme	non-endospore-forming	166:186	arg1	bacterium					188:196	A novel Gram-stain-variable, rod-shaped, non-motile and non-endospore-forming bacterium	110:196	A novel Gram-stain-variable, rod-shaped, non-motile and non-endospore-forming bacterium (strain G27T)	110:210	A novel Gram-stain-variable, rod-shaped, non-motile and non-endospore-forming bacterium (strain G27T) was isolated from near Dhuvaran, Gujarat, India.
30028282	2	39	theme	non-endospore-forming	166:186	arg1	G27T					206:209	strain G27T	199:209	strain G27T	199:209	A novel Gram-stain-variable, rod-shaped, non-motile and non-endospore-forming bacterium (strain G27T) was isolated from near Dhuvaran, Gujarat, India.
30028282	3	40	theme	populi	410:415	arg1	similarity					446:455	94.9 % sequence similarity	430:455	94.9 % sequence similarity	430:455	Based on 16S rRNA gene sequence analysis, strain G27T was identified as a member of the class Firmibacteria and was most closely related to Bacillus populi FJAT-45347T (94.9 % sequence similarity), Salipaludibacillus aurantiacus S9T (94.9 %), Salipaludibacillus neizhouensis KCTC 13187T (94.7 %), Alteribacillus iranensis DSM 23995T (94.6 %) and other Firmibacteria (<94.6 %).
30028282	3	40	theme	populi	410:415	arg1	FJAT-45347T					417:427	Bacillus populi FJAT-45347T	401:427	Bacillus populi FJAT-45347T (94.9 % sequence similarity)	401:456	Based on 16S rRNA gene sequence analysis, strain G27T was identified as a member of the class Firmibacteria and was most closely related to Bacillus populi FJAT-45347T (94.9 % sequence similarity), Salipaludibacillus aurantiacus S9T (94.9 %), Salipaludibacillus neizhouensis KCTC 13187T (94.7 %), Alteribacillus iranensis DSM 23995T (94.6 %) and other Firmibacteria (<94.6 %).
30028282	9	41	theme	genus	1298:1302	arg1	species					1281:1287	a novel species	1273:1287	a novel species of a new genus, for which the name Thalassorhabdus alkalitolerans gen. nov., sp	1273:1367	The results of phylogenetic, chemotaxonomic and biochemical tests allowed the clear differentiation of strain G27T from all other members of the family Bacillaceae.It is therefore considered to represent a novel species of a new genus, for which the name Thalassorhabdus alkalitolerans gen. nov., sp.
30028282	3	42	theme	DSM	583:585	arg1	23995T					587:592	Alteribacillus iranensis DSM 23995T	558:592	Alteribacillus iranensis DSM 23995T (94.6 %)	558:601	Based on 16S rRNA gene sequence analysis, strain G27T was identified as a member of the class Firmibacteria and was most closely related to Bacillus populi FJAT-45347T (94.9 % sequence similarity), Salipaludibacillus aurantiacus S9T (94.9 %), Salipaludibacillus neizhouensis KCTC 13187T (94.7 %), Alteribacillus iranensis DSM 23995T (94.6 %) and other Firmibacteria (<94.6 %).
30028282	3	42	theme	DSM	583:585	arg1	%					600:600	94.6 %	595:600	94.6 %	595:600	Based on 16S rRNA gene sequence analysis, strain G27T was identified as a member of the class Firmibacteria and was most closely related to Bacillus populi FJAT-45347T (94.9 % sequence similarity), Salipaludibacillus aurantiacus S9T (94.9 %), Salipaludibacillus neizhouensis KCTC 13187T (94.7 %), Alteribacillus iranensis DSM 23995T (94.6 %) and other Firmibacteria (<94.6 %).
30028282	3	43	theme	Firmibacteria	355:367	arg1	member					335:340	a member	333:340	a member of the class Firmibacteria	333:367	Based on 16S rRNA gene sequence analysis, strain G27T was identified as a member of the class Firmibacteria and was most closely related to Bacillus populi FJAT-45347T (94.9 % sequence similarity), Salipaludibacillus aurantiacus S9T (94.9 %), Salipaludibacillus neizhouensis KCTC 13187T (94.7 %), Alteribacillus iranensis DSM 23995T (94.6 %) and other Firmibacteria (<94.6 %).
30028282	3	43	theme	Firmibacteria	355:367	arg1	G27T					310:313	strain G27T	303:313	strain G27T	303:313	Based on 16S rRNA gene sequence analysis, strain G27T was identified as a member of the class Firmibacteria and was most closely related to Bacillus populi FJAT-45347T (94.9 % sequence similarity), Salipaludibacillus aurantiacus S9T (94.9 %), Salipaludibacillus neizhouensis KCTC 13187T (94.7 %), Alteribacillus iranensis DSM 23995T (94.6 %) and other Firmibacteria (<94.6 %).
30028282	3	44	theme	gene	279:282	arg1	analysis					293:300	16S rRNA gene sequence analysis	270:300	16S rRNA gene sequence analysis	270:300	Based on 16S rRNA gene sequence analysis, strain G27T was identified as a member of the class Firmibacteria and was most closely related to Bacillus populi FJAT-45347T (94.9 % sequence similarity), Salipaludibacillus aurantiacus S9T (94.9 %), Salipaludibacillus neizhouensis KCTC 13187T (94.7 %), Alteribacillus iranensis DSM 23995T (94.6 %) and other Firmibacteria (<94.6 %).
30028282	2	45	theme	non-motile	151:160	arg1	bacterium					188:196	A novel Gram-stain-variable, rod-shaped, non-motile and non-endospore-forming bacterium	110:196	A novel Gram-stain-variable, rod-shaped, non-motile and non-endospore-forming bacterium (strain G27T)	110:210	A novel Gram-stain-variable, rod-shaped, non-motile and non-endospore-forming bacterium (strain G27T) was isolated from near Dhuvaran, Gujarat, India.
30028282	2	45	theme	non-motile	151:160	arg1	G27T					206:209	strain G27T	199:209	strain G27T	199:209	A novel Gram-stain-variable, rod-shaped, non-motile and non-endospore-forming bacterium (strain G27T) was isolated from near Dhuvaran, Gujarat, India.
30028282	3	46	theme	sequence	284:291	arg1	analysis					293:300	16S rRNA gene sequence analysis	270:300	16S rRNA gene sequence analysis	270:300	Based on 16S rRNA gene sequence analysis, strain G27T was identified as a member of the class Firmibacteria and was most closely related to Bacillus populi FJAT-45347T (94.9 % sequence similarity), Salipaludibacillus aurantiacus S9T (94.9 %), Salipaludibacillus neizhouensis KCTC 13187T (94.7 %), Alteribacillus iranensis DSM 23995T (94.6 %) and other Firmibacteria (<94.6 %).
30028282	9	47	theme	other	1193:1197	arg1	members					1199:1205	all other members	1189:1205	all other members of the family Bacillaceae.It is therefore considered to represent a novel species of a new genus, for which the name Thalassorhabdus alkalitolerans gen. nov., sp	1189:1367	The results of phylogenetic, chemotaxonomic and biochemical tests allowed the clear differentiation of strain G27T from all other members of the family Bacillaceae.It is therefore considered to represent a novel species of a new genus, for which the name Thalassorhabdus alkalitolerans gen. nov., sp.
30028282	11	48	theme	Thalassorhabdus	1408:1422	arg1	alkalitolerans					1424:1437	Thalassorhabdus alkalitolerans	1408:1437	Thalassorhabdus alkalitolerans	1408:1437	The type strain of Thalassorhabdus alkalitolerans is G27T (=MCC 3411T=CGMCC 1.15772T=KCTC 33941T).
30028282	3	49	theme	16S	270:272	arg1	analysis					293:300	16S rRNA gene sequence analysis	270:300	16S rRNA gene sequence analysis	270:300	Based on 16S rRNA gene sequence analysis, strain G27T was identified as a member of the class Firmibacteria and was most closely related to Bacillus populi FJAT-45347T (94.9 % sequence similarity), Salipaludibacillus aurantiacus S9T (94.9 %), Salipaludibacillus neizhouensis KCTC 13187T (94.7 %), Alteribacillus iranensis DSM 23995T (94.6 %) and other Firmibacteria (<94.6 %).
30028282	3	50	theme	other	607:611	arg1	Firmibacteria					613:625	other Firmibacteria	607:625	other Firmibacteria (<94.6 %)	607:635	Based on 16S rRNA gene sequence analysis, strain G27T was identified as a member of the class Firmibacteria and was most closely related to Bacillus populi FJAT-45347T (94.9 % sequence similarity), Salipaludibacillus aurantiacus S9T (94.9 %), Salipaludibacillus neizhouensis KCTC 13187T (94.7 %), Alteribacillus iranensis DSM 23995T (94.6 %) and other Firmibacteria (<94.6 %).
30028282	3	50	theme	other	607:611	arg1	%					634:634	<94.6 %	628:634	<94.6 %	628:634	Based on 16S rRNA gene sequence analysis, strain G27T was identified as a member of the class Firmibacteria and was most closely related to Bacillus populi FJAT-45347T (94.9 % sequence similarity), Salipaludibacillus aurantiacus S9T (94.9 %), Salipaludibacillus neizhouensis KCTC 13187T (94.7 %), Alteribacillus iranensis DSM 23995T (94.6 %) and other Firmibacteria (<94.6 %).
30028282	3	51	theme	Alteribacillus	558:571	arg1	23995T					587:592	Alteribacillus iranensis DSM 23995T	558:592	Alteribacillus iranensis DSM 23995T (94.6 %)	558:601	Based on 16S rRNA gene sequence analysis, strain G27T was identified as a member of the class Firmibacteria and was most closely related to Bacillus populi FJAT-45347T (94.9 % sequence similarity), Salipaludibacillus aurantiacus S9T (94.9 %), Salipaludibacillus neizhouensis KCTC 13187T (94.7 %), Alteribacillus iranensis DSM 23995T (94.6 %) and other Firmibacteria (<94.6 %).
30028282	3	51	theme	Alteribacillus	558:571	arg1	%					600:600	94.6 %	595:600	94.6 %	595:600	Based on 16S rRNA gene sequence analysis, strain G27T was identified as a member of the class Firmibacteria and was most closely related to Bacillus populi FJAT-45347T (94.9 % sequence similarity), Salipaludibacillus aurantiacus S9T (94.9 %), Salipaludibacillus neizhouensis KCTC 13187T (94.7 %), Alteribacillus iranensis DSM 23995T (94.6 %) and other Firmibacteria (<94.6 %).
30028282	3	52	theme	%	435:435	arg1	similarity					446:455	94.9 % sequence similarity	430:455	94.9 % sequence similarity	430:455	Based on 16S rRNA gene sequence analysis, strain G27T was identified as a member of the class Firmibacteria and was most closely related to Bacillus populi FJAT-45347T (94.9 % sequence similarity), Salipaludibacillus aurantiacus S9T (94.9 %), Salipaludibacillus neizhouensis KCTC 13187T (94.7 %), Alteribacillus iranensis DSM 23995T (94.6 %) and other Firmibacteria (<94.6 %).
30028282	3	52	theme	%	435:435	arg1	FJAT-45347T					417:427	Bacillus populi FJAT-45347T	401:427	Bacillus populi FJAT-45347T (94.9 % sequence similarity)	401:456	Based on 16S rRNA gene sequence analysis, strain G27T was identified as a member of the class Firmibacteria and was most closely related to Bacillus populi FJAT-45347T (94.9 % sequence similarity), Salipaludibacillus aurantiacus S9T (94.9 %), Salipaludibacillus neizhouensis KCTC 13187T (94.7 %), Alteribacillus iranensis DSM 23995T (94.6 %) and other Firmibacteria (<94.6 %).
30028282	11	53	theme	alkalitolerans	1424:1437	arg1	G27T					1442:1445	G27T	1442:1445	G27T (=MCC 3411T=CGMCC 1.15772T=KCTC 33941T)	1442:1485	The type strain of Thalassorhabdus alkalitolerans is G27T (=MCC 3411T=CGMCC 1.15772T=KCTC 33941T).
30028282	11	53	theme	alkalitolerans	1424:1437	arg1	strain					1398:1403	The type strain	1389:1403	The type strain of Thalassorhabdus alkalitolerans	1389:1437	The type strain of Thalassorhabdus alkalitolerans is G27T (=MCC 3411T=CGMCC 1.15772T=KCTC 33941T).
30028282	3	54	theme	rRNA	274:277	arg1	analysis					293:300	16S rRNA gene sequence analysis	270:300	16S rRNA gene sequence analysis	270:300	Based on 16S rRNA gene sequence analysis, strain G27T was identified as a member of the class Firmibacteria and was most closely related to Bacillus populi FJAT-45347T (94.9 % sequence similarity), Salipaludibacillus aurantiacus S9T (94.9 %), Salipaludibacillus neizhouensis KCTC 13187T (94.7 %), Alteribacillus iranensis DSM 23995T (94.6 %) and other Firmibacteria (<94.6 %).
30028282	2	55	theme	strain	199:204	arg1	bacterium					188:196	A novel Gram-stain-variable, rod-shaped, non-motile and non-endospore-forming bacterium	110:196	A novel Gram-stain-variable, rod-shaped, non-motile and non-endospore-forming bacterium (strain G27T)	110:210	A novel Gram-stain-variable, rod-shaped, non-motile and non-endospore-forming bacterium (strain G27T) was isolated from near Dhuvaran, Gujarat, India.
30028282	2	55	theme	strain	199:204	arg1	G27T					206:209	strain G27T	199:209	strain G27T	199:209	A novel Gram-stain-variable, rod-shaped, non-motile and non-endospore-forming bacterium (strain G27T) was isolated from near Dhuvaran, Gujarat, India.
30028282	3	56	theme	iranensis	573:581	arg1	23995T					587:592	Alteribacillus iranensis DSM 23995T	558:592	Alteribacillus iranensis DSM 23995T (94.6 %)	558:601	Based on 16S rRNA gene sequence analysis, strain G27T was identified as a member of the class Firmibacteria and was most closely related to Bacillus populi FJAT-45347T (94.9 % sequence similarity), Salipaludibacillus aurantiacus S9T (94.9 %), Salipaludibacillus neizhouensis KCTC 13187T (94.7 %), Alteribacillus iranensis DSM 23995T (94.6 %) and other Firmibacteria (<94.6 %).
30028282	3	56	theme	iranensis	573:581	arg1	%					600:600	94.6 %	595:600	94.6 %	595:600	Based on 16S rRNA gene sequence analysis, strain G27T was identified as a member of the class Firmibacteria and was most closely related to Bacillus populi FJAT-45347T (94.9 % sequence similarity), Salipaludibacillus aurantiacus S9T (94.9 %), Salipaludibacillus neizhouensis KCTC 13187T (94.7 %), Alteribacillus iranensis DSM 23995T (94.6 %) and other Firmibacteria (<94.6 %).
30028282	9	57	theme	phylogenetic	1084:1095	arg1	tests					1129:1133	phylogenetic, chemotaxonomic and biochemical tests	1084:1133	phylogenetic, chemotaxonomic and biochemical tests	1084:1133	The results of phylogenetic, chemotaxonomic and biochemical tests allowed the clear differentiation of strain G27T from all other members of the family Bacillaceae.It is therefore considered to represent a novel species of a new genus, for which the name Thalassorhabdus alkalitolerans gen. nov., sp.
30028282	3	58	theme	sequence	437:444	arg1	similarity					446:455	94.9 % sequence similarity	430:455	94.9 % sequence similarity	430:455	Based on 16S rRNA gene sequence analysis, strain G27T was identified as a member of the class Firmibacteria and was most closely related to Bacillus populi FJAT-45347T (94.9 % sequence similarity), Salipaludibacillus aurantiacus S9T (94.9 %), Salipaludibacillus neizhouensis KCTC 13187T (94.7 %), Alteribacillus iranensis DSM 23995T (94.6 %) and other Firmibacteria (<94.6 %).
30028282	3	58	theme	sequence	437:444	arg1	FJAT-45347T					417:427	Bacillus populi FJAT-45347T	401:427	Bacillus populi FJAT-45347T (94.9 % sequence similarity)	401:456	Based on 16S rRNA gene sequence analysis, strain G27T was identified as a member of the class Firmibacteria and was most closely related to Bacillus populi FJAT-45347T (94.9 % sequence similarity), Salipaludibacillus aurantiacus S9T (94.9 %), Salipaludibacillus neizhouensis KCTC 13187T (94.7 %), Alteribacillus iranensis DSM 23995T (94.6 %) and other Firmibacteria (<94.6 %).
30028282	4	59	theme	DNA	642:644	arg1	content					650:656	The DNA G+C content	638:656	The DNA G+C content of strain G27T	638:671	The DNA G+C content of strain G27T was 43.4±0.6 mol%.
30028282	4	59	theme	DNA	642:644	arg1	%					689:689	43.4±0.6 mol%	677:689	43.4±0.6 mol%	677:689	The DNA G+C content of strain G27T was 43.4±0.6 mol%.
30028282	3	60	theme	strain	303:308	arg1	member					335:340	a member	333:340	a member of the class Firmibacteria	333:367	Based on 16S rRNA gene sequence analysis, strain G27T was identified as a member of the class Firmibacteria and was most closely related to Bacillus populi FJAT-45347T (94.9 % sequence similarity), Salipaludibacillus aurantiacus S9T (94.9 %), Salipaludibacillus neizhouensis KCTC 13187T (94.7 %), Alteribacillus iranensis DSM 23995T (94.6 %) and other Firmibacteria (<94.6 %).
30028282	3	60	theme	strain	303:308	arg1	G27T					310:313	strain G27T	303:313	strain G27T	303:313	Based on 16S rRNA gene sequence analysis, strain G27T was identified as a member of the class Firmibacteria and was most closely related to Bacillus populi FJAT-45347T (94.9 % sequence similarity), Salipaludibacillus aurantiacus S9T (94.9 %), Salipaludibacillus neizhouensis KCTC 13187T (94.7 %), Alteribacillus iranensis DSM 23995T (94.6 %) and other Firmibacteria (<94.6 %).
30028282	6	61	theme	Polar	756:760	arg1	lipids					762:767	Polar lipids	756:767	Polar lipids	756:767	Polar lipids included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unidentified phospholipid and five unidentified lipids.
30028282	5	62	theme	cell-wall	696:704	arg1	peptidoglycan					706:718	The cell-wall peptidoglycan	692:718	The cell-wall peptidoglycan	692:718	The cell-wall peptidoglycan contained meso-diaminopimelic acid.
30028282	2	63	theme	novel	112:116	arg1	bacterium					188:196	A novel Gram-stain-variable, rod-shaped, non-motile and non-endospore-forming bacterium	110:196	A novel Gram-stain-variable, rod-shaped, non-motile and non-endospore-forming bacterium (strain G27T)	110:210	A novel Gram-stain-variable, rod-shaped, non-motile and non-endospore-forming bacterium (strain G27T) was isolated from near Dhuvaran, Gujarat, India.
30028282	2	63	theme	novel	112:116	arg1	G27T					206:209	strain G27T	199:209	strain G27T	199:209	A novel Gram-stain-variable, rod-shaped, non-motile and non-endospore-forming bacterium (strain G27T) was isolated from near Dhuvaran, Gujarat, India.
30028282	9	64	theme	chemotaxonomic	1098:1111	arg1	tests					1129:1133	phylogenetic, chemotaxonomic and biochemical tests	1084:1133	phylogenetic, chemotaxonomic and biochemical tests	1084:1133	The results of phylogenetic, chemotaxonomic and biochemical tests allowed the clear differentiation of strain G27T from all other members of the family Bacillaceae.It is therefore considered to represent a novel species of a new genus, for which the name Thalassorhabdus alkalitolerans gen. nov., sp.
30028282	3	65	dep	Salipaludibacillus	504:521	arg1	neizhouensis					523:534	neizhouensis	523:534	neizhouensis	523:534	Based on 16S rRNA gene sequence analysis, strain G27T was identified as a member of the class Firmibacteria and was most closely related to Bacillus populi FJAT-45347T (94.9 % sequence similarity), Salipaludibacillus aurantiacus S9T (94.9 %), Salipaludibacillus neizhouensis KCTC 13187T (94.7 %), Alteribacillus iranensis DSM 23995T (94.6 %) and other Firmibacteria (<94.6 %).
30028282	11	66	theme	1.15772T=KCTC	1465:1477	arg1	33941T					1479:1484	=MCC 3411T=CGMCC 1.15772T=KCTC 33941T	1448:1484	=MCC 3411T=CGMCC 1.15772T=KCTC 33941T	1448:1484	The type strain of Thalassorhabdus alkalitolerans is G27T (=MCC 3411T=CGMCC 1.15772T=KCTC 33941T).
30028282	11	66	theme	1.15772T=KCTC	1465:1477	arg1	G27T					1442:1445	G27T	1442:1445	G27T (=MCC 3411T=CGMCC 1.15772T=KCTC 33941T)	1442:1485	The type strain of Thalassorhabdus alkalitolerans is G27T (=MCC 3411T=CGMCC 1.15772T=KCTC 33941T).
30831211	10	0	theme	Genes	1479:1483	arg1	number					1444:1449	The highest number	1432:1449	The highest number of Differentially Expressed Genes (DEG)	1432:1489	The highest number of Differentially Expressed Genes (DEG) was detected in cecal tonsils (160 DEG) after S1 in ovo injection, and in liver (159 DEG) after S2 injection.
30831211	2	1	theme	incubation	336:345	arg1	day					321:323	day 12	321:326	day 12 of eggs incubation	321:345	In poultry, synbiotics can be used to reprogram animal's intestinal microbiota upon perinatal in ovo injection on day 12 of eggs incubation.
30831211	12	2	from	pathways	1898:1905	arg1	liver					1914:1918	the liver	1910:1918	the liver	1910:1918	S2 injection led to modulation of the gene expression associated with metabolic and developmental signaling pathways in the liver.
30831211	4	3	theme	long-term	582:590	arg1	changes					592:598	long-term changes	582:598	long-term changes in the chicken transcriptome	582:627	The aim of the study was to estimate long-term changes in the chicken transcriptome after a single in ovo administration of two different synbiotics.
30831211	12	4	theme	signaling	1888:1896	arg1	pathways					1898:1905	metabolic and developmental signaling pathways	1860:1905	metabolic and developmental signaling pathways in the liver	1860:1918	S2 injection led to modulation of the gene expression associated with metabolic and developmental signaling pathways in the liver.
30831211	2	5	theme	intestinal	264:273	arg1	microbiota					275:284	animal's intestinal microbiota	255:284	animal's intestinal microbiota	255:284	In poultry, synbiotics can be used to reprogram animal's intestinal microbiota upon perinatal in ovo injection on day 12 of eggs incubation.
30831211	1	6	theme	prebiotics	180:189	arg1	effects					169:175	effects	169:175	effects of prebiotics and probiotics	169:204	Synbiotics are the bioactive compounds that synergistically combine effects of prebiotics and probiotics.
30831211	12	7	theme	expression	1833:1842	arg1	modulation					1810:1819	modulation	1810:1819	modulation of the gene expression associated with metabolic and developmental signaling pathways in the liver	1810:1918	S2 injection led to modulation of the gene expression associated with metabolic and developmental signaling pathways in the liver.
30831211	6	8	theme	metabolic	1102:1110	arg1	tissues					1120:1126	immunological (cecal tonsils and spleen), intestinal (jejunum) and metabolic (liver) tissues	1035:1126	immunological (cecal tonsils and spleen), intestinal (jejunum) and metabolic (liver) tissues	1035:1126	On day 21 post-hatching cockerels were sacrificed and immunological (cecal tonsils and spleen), intestinal (jejunum) and metabolic (liver) tissues were collected (n = 5).
30831211	6	9	dep	metabolic	1102:1110	arg1	liver					1113:1117	liver	1113:1117	liver	1113:1117	On day 21 post-hatching cockerels were sacrificed and immunological (cecal tonsils and spleen), intestinal (jejunum) and metabolic (liver) tissues were collected (n = 5).
30831211	9	10	theme	Venn	1394:1397	arg1	diagrams					1399:1406	Venn diagrams	1394:1406	Venn diagrams	1394:1406	Data analysis was performed using Affymetrix Expression Console and Transcriptome Analysis Console software, Venn diagrams, DAVID and CateGOrizer.
30831211	13	11	theme	significant	1992:2002	arg1	impact					2004:2009	significant impact	1992:2009	significant impact	1992:2009	Obtained results let us conclude that synbiotics delivered in ovo have significant impact on chicken transcriptome and their effect depends on the composition of the bioactive compound.
30831211	2	12	theme	in	301:302	arg1	injection					308:316	perinatal in ovo injection	291:316	perinatal in ovo injection on day 12 of eggs incubation	291:345	In poultry, synbiotics can be used to reprogram animal's intestinal microbiota upon perinatal in ovo injection on day 12 of eggs incubation.
30831211	2	13	used	used	237:240	arg2	synbiotics					219:228	synbiotics	219:228	synbiotics	219:228	In poultry, synbiotics can be used to reprogram animal's intestinal microbiota upon perinatal in ovo injection on day 12 of eggs incubation.
30831211	4	14	theme	single	637:642	arg1	administration					651:664	a single in ovo administration	635:664	a single in ovo administration of two different synbiotics	635:692	The aim of the study was to estimate long-term changes in the chicken transcriptome after a single in ovo administration of two different synbiotics.
30831211	10	15	theme	S1	1537:1538	arg1	injection					1547:1555	S1 in ovo injection	1537:1555	S1 in ovo injection	1537:1555	The highest number of Differentially Expressed Genes (DEG) was detected in cecal tonsils (160 DEG) after S1 in ovo injection, and in liver (159 DEG) after S2 injection.
30831211	6	16	dep	collected	1133:1141	arg1	n = 5					1144:1148	n = 5	1144:1148	n = 5	1144:1148	On day 21 post-hatching cockerels were sacrificed and immunological (cecal tonsils and spleen), intestinal (jejunum) and metabolic (liver) tissues were collected (n = 5).
30831211	9	17	dep	Analysis	1367:1374	arg1	software					1384:1391	Console software	1376:1391	Console software	1376:1391	Data analysis was performed using Affymetrix Expression Console and Transcriptome Analysis Console software, Venn diagrams, DAVID and CateGOrizer.
30831211	4	18	theme	different	673:681	arg1	synbiotics					683:692	two different synbiotics	669:692	two different synbiotics	669:692	The aim of the study was to estimate long-term changes in the chicken transcriptome after a single in ovo administration of two different synbiotics.
30831211	8	19	theme	Array	1259:1263	arg1	Affymetrix					1272:1281	Affymetrix	1272:1281	Affymetrix	1272:1281	ST Array Strip (Affymetrix).
30831211	8	19	theme	Array	1259:1263	arg1	Strip					1265:1269	ST Array Strip	1256:1269	ST Array Strip (Affymetrix)	1256:1282	ST Array Strip (Affymetrix).
30831211	4	20	from	changes	592:598	arg1	transcriptome					615:627	the chicken transcriptome	603:627	the chicken transcriptome	603:627	The aim of the study was to estimate long-term changes in the chicken transcriptome after a single in ovo administration of two different synbiotics.
30831211	11	21	from	metabolism	1741:1750	arg1	tonsils					1781:1787	cecal tonsils	1775:1787	cecal tonsils	1775:1787	The influence of S1 on transcriptome modulation was demonstrated by a strong activation of the genes taking part in the pathways related to metabolism and immune response in cecal tonsils.
30831211	0	22	theme	chicken	84:90	arg1	tissues					92:98	chicken tissues	84:98	chicken tissues	84:98	Transcriptome modulation by in ovo delivered Lactobacillus synbiotics in a range of chicken tissues.
30831211	11	23	theme	cecal	1775:1779	arg1	tonsils					1781:1787	cecal tonsils	1775:1787	cecal tonsils	1775:1787	The influence of S1 on transcriptome modulation was demonstrated by a strong activation of the genes taking part in the pathways related to metabolism and immune response in cecal tonsils.
30831211	5	24	theme	raffinose	940:948	arg1	oligosaccharides					957:972	raffinose family oligosaccharides	940:972	raffinose family oligosaccharides	940:972	On day 12 of eggs incubation, 5850 eggs of broiler chicken were distributed to experimental groups and injected with synbiotic 1 (S1)- Lactobacillus salivarius with galactooligosaccharides (GOS) or synbiotic 2 (S2)- Lactobacillus plantarum with raffinose family oligosaccharides (RFO).
30831211	6	25	theme	immunological	1035:1047	arg1	tissues					1120:1126	immunological (cecal tonsils and spleen), intestinal (jejunum) and metabolic (liver) tissues	1035:1126	immunological (cecal tonsils and spleen), intestinal (jejunum) and metabolic (liver) tissues	1035:1126	On day 21 post-hatching cockerels were sacrificed and immunological (cecal tonsils and spleen), intestinal (jejunum) and metabolic (liver) tissues were collected (n = 5).
30831211	0	26	theme	Transcriptome	0:12	arg1	modulation					14:23	Transcriptome modulation	0:23	Transcriptome modulation by in ovo delivered Lactobacillus synbiotics in a range of chicken tissues.	0:99	Transcriptome modulation by in ovo delivered Lactobacillus synbiotics in a range of chicken tissues.
30831211	13	27	contain	have	1987:1990	arg2	impact					2004:2009	significant impact	1992:2009	significant impact	1992:2009	Obtained results let us conclude that synbiotics delivered in ovo have significant impact on chicken transcriptome and their effect depends on the composition of the bioactive compound.
30831211	13	27	contain	have	1987:1990	arg1	synbiotics					1959:1968	synbiotics	1959:1968	synbiotics delivered in ovo	1959:1985	Obtained results let us conclude that synbiotics delivered in ovo have significant impact on chicken transcriptome and their effect depends on the composition of the bioactive compound.
30831211	10	28	theme	highest	1436:1442	arg1	number					1444:1449	The highest number	1432:1449	The highest number of Differentially Expressed Genes (DEG)	1432:1489	The highest number of Differentially Expressed Genes (DEG) was detected in cecal tonsils (160 DEG) after S1 in ovo injection, and in liver (159 DEG) after S2 injection.
30831211	0	29	theme	ovo	31:33	arg1	synbiotics					59:68	in ovo delivered Lactobacillus synbiotics	28:68	in ovo delivered Lactobacillus synbiotics	28:68	Transcriptome modulation by in ovo delivered Lactobacillus synbiotics in a range of chicken tissues.
30831211	7	30	theme	GeneChip	1229:1236	arg1	Gene					1246:1249	GeneChip Chicken Gene 1.1	1229:1253	GeneChip Chicken Gene 1.1	1229:1253	Isolated RNA served as a template for the whole-transcriptome analysis using GeneChip Chicken Gene 1.1.
30831211	13	31	dep	let	1938:1940	arg1	us					1942:1943	us	1942:1943	us	1942:1943	Obtained results let us conclude that synbiotics delivered in ovo have significant impact on chicken transcriptome and their effect depends on the composition of the bioactive compound.
30831211	10	32	theme	Expressed	1469:1477	arg1	DEG					1486:1488	DEG	1486:1488	DEG	1486:1488	The highest number of Differentially Expressed Genes (DEG) was detected in cecal tonsils (160 DEG) after S1 in ovo injection, and in liver (159 DEG) after S2 injection.
30831211	10	32	theme	Expressed	1469:1477	arg1	Genes					1479:1483	Differentially Expressed Genes	1454:1483	Differentially Expressed Genes (DEG)	1454:1489	The highest number of Differentially Expressed Genes (DEG) was detected in cecal tonsils (160 DEG) after S1 in ovo injection, and in liver (159 DEG) after S2 injection.
30831211	10	33	theme	S2	1587:1588	arg1	injection					1590:1598	S2 injection	1587:1598	S2 injection	1587:1598	The highest number of Differentially Expressed Genes (DEG) was detected in cecal tonsils (160 DEG) after S1 in ovo injection, and in liver (159 DEG) after S2 injection.
30831211	5	34	theme	experimental	774:785	arg1	groups					787:792	experimental groups	774:792	experimental groups	774:792	On day 12 of eggs incubation, 5850 eggs of broiler chicken were distributed to experimental groups and injected with synbiotic 1 (S1)- Lactobacillus salivarius with galactooligosaccharides (GOS) or synbiotic 2 (S2)- Lactobacillus plantarum with raffinose family oligosaccharides (RFO).
30831211	13	35	theme	bioactive	2087:2095	arg1	compound					2097:2104	the bioactive compound	2083:2104	the bioactive compound	2083:2104	Obtained results let us conclude that synbiotics delivered in ovo have significant impact on chicken transcriptome and their effect depends on the composition of the bioactive compound.
30831211	6	36	theme	intestinal	1077:1086	arg1	tissues					1120:1126	immunological (cecal tonsils and spleen), intestinal (jejunum) and metabolic (liver) tissues	1035:1126	immunological (cecal tonsils and spleen), intestinal (jejunum) and metabolic (liver) tissues	1035:1126	On day 21 post-hatching cockerels were sacrificed and immunological (cecal tonsils and spleen), intestinal (jejunum) and metabolic (liver) tissues were collected (n = 5).
30831211	3	37	theme	beneficial	472:481	arg1	effects					483:489	beneficial effects	472:489	beneficial effects	472:489	Optimally composed synbiotic delivered in ovo efficiently stimulates the host's intestinal microflora, which in turn exerts beneficial effects on the host and improves its physiological functions.
30831211	9	38	theme	Affymetrix	1319:1328	arg1	Analysis					1367:1374	Affymetrix Expression Console and Transcriptome Analysis Console software, Venn diagrams, DAVID and CateGOrizer	1319:1429	Analysis	1367:1374	Data analysis was performed using Affymetrix Expression Console and Transcriptome Analysis Console software, Venn diagrams, DAVID and CateGOrizer.
30831211	11	39	theme	S1	1618:1619	arg1	influence					1605:1613	The influence	1601:1613	The influence of S1 on transcriptome modulation	1601:1647	The influence of S1 on transcriptome modulation was demonstrated by a strong activation of the genes taking part in the pathways related to metabolism and immune response in cecal tonsils.
30831211	9	40	theme	Console	1341:1347	arg1	Analysis					1367:1374	Affymetrix Expression Console and Transcriptome Analysis Console software, Venn diagrams, DAVID and CateGOrizer	1319:1429	Analysis	1367:1374	Data analysis was performed using Affymetrix Expression Console and Transcriptome Analysis Console software, Venn diagrams, DAVID and CateGOrizer.
30831211	6	41	theme	cecal	1050:1054	arg1	tonsils					1056:1062	cecal tonsils	1050:1062	cecal tonsils	1050:1062	On day 21 post-hatching cockerels were sacrificed and immunological (cecal tonsils and spleen), intestinal (jejunum) and metabolic (liver) tissues were collected (n = 5).
30831211	10	42	theme	cecal	1507:1511	arg1	tonsils					1513:1519	cecal tonsils	1507:1519	cecal tonsils (160 DEG)	1507:1529	The highest number of Differentially Expressed Genes (DEG) was detected in cecal tonsils (160 DEG) after S1 in ovo injection, and in liver (159 DEG) after S2 injection.
30831211	10	42	theme	cecal	1507:1511	arg1	DEG					1526:1528	160 DEG	1522:1528	160 DEG	1522:1528	The highest number of Differentially Expressed Genes (DEG) was detected in cecal tonsils (160 DEG) after S1 in ovo injection, and in liver (159 DEG) after S2 injection.
30831211	10	43	dep	in	1540:1541	arg1	ovo					1543:1545	ovo	1543:1545	ovo	1543:1545	The highest number of Differentially Expressed Genes (DEG) was detected in cecal tonsils (160 DEG) after S1 in ovo injection, and in liver (159 DEG) after S2 injection.
30831211	2	44	theme	perinatal	291:299	arg1	injection					308:316	perinatal in ovo injection	291:316	perinatal in ovo injection on day 12 of eggs incubation	291:345	In poultry, synbiotics can be used to reprogram animal's intestinal microbiota upon perinatal in ovo injection on day 12 of eggs incubation.
30831211	9	45	theme	Transcriptome	1353:1365	arg1	Analysis					1367:1374	Affymetrix Expression Console and Transcriptome Analysis Console software, Venn diagrams, DAVID and CateGOrizer	1319:1429	Analysis	1367:1374	Data analysis was performed using Affymetrix Expression Console and Transcriptome Analysis Console software, Venn diagrams, DAVID and CateGOrizer.
30831211	12	46	theme	metabolic	1860:1868	arg1	pathways					1898:1905	metabolic and developmental signaling pathways	1860:1905	metabolic and developmental signaling pathways in the liver	1860:1918	S2 injection led to modulation of the gene expression associated with metabolic and developmental signaling pathways in the liver.
30831211	2	47	theme	eggs	331:334	arg1	incubation					336:345	eggs incubation	331:345	eggs incubation	331:345	In poultry, synbiotics can be used to reprogram animal's intestinal microbiota upon perinatal in ovo injection on day 12 of eggs incubation.
30831211	3	48	theme	physiological	520:532	arg1	functions					534:542	its physiological functions	516:542	its physiological functions	516:542	Optimally composed synbiotic delivered in ovo efficiently stimulates the host's intestinal microflora, which in turn exerts beneficial effects on the host and improves its physiological functions.
30831211	9	49	theme	Console	1376:1382	arg1	software					1384:1391	Console software	1376:1391	Console software	1376:1391	Data analysis was performed using Affymetrix Expression Console and Transcriptome Analysis Console software, Venn diagrams, DAVID and CateGOrizer.
30831211	0	50	theme	Lactobacillus	45:57	arg1	synbiotics					59:68	in ovo delivered Lactobacillus synbiotics	28:68	in ovo delivered Lactobacillus synbiotics	28:68	Transcriptome modulation by in ovo delivered Lactobacillus synbiotics in a range of chicken tissues.
30831211	4	51	theme	in	644:645	arg1	administration					651:664	a single in ovo administration	635:664	a single in ovo administration of two different synbiotics	635:692	The aim of the study was to estimate long-term changes in the chicken transcriptome after a single in ovo administration of two different synbiotics.
30831211	5	52	theme	chicken	746:752	arg1	eggs					730:733	5850 eggs	725:733	5850 eggs of broiler chicken	725:752	On day 12 of eggs incubation, 5850 eggs of broiler chicken were distributed to experimental groups and injected with synbiotic 1 (S1)- Lactobacillus salivarius with galactooligosaccharides (GOS) or synbiotic 2 (S2)- Lactobacillus plantarum with raffinose family oligosaccharides (RFO).
30831211	12	53	theme	gene	1828:1831	arg1	expression					1833:1842	the gene expression	1824:1842	the gene expression associated with metabolic and developmental signaling pathways in the liver	1824:1918	S2 injection led to modulation of the gene expression associated with metabolic and developmental signaling pathways in the liver.
30831211	8	54	theme	ST	1256:1257	arg1	Affymetrix					1272:1281	Affymetrix	1272:1281	Affymetrix	1272:1281	ST Array Strip (Affymetrix).
30831211	8	54	theme	ST	1256:1257	arg1	Strip					1265:1269	ST Array Strip	1256:1269	ST Array Strip (Affymetrix)	1256:1282	ST Array Strip (Affymetrix).
30831211	10	55	theme	in	1540:1541	arg1	injection					1547:1555	S1 in ovo injection	1537:1555	S1 in ovo injection	1537:1555	The highest number of Differentially Expressed Genes (DEG) was detected in cecal tonsils (160 DEG) after S1 in ovo injection, and in liver (159 DEG) after S2 injection.
30831211	11	56	theme	strong	1671:1676	arg1	activation					1678:1687	a strong activation	1669:1687	a strong activation of the genes taking part in the pathways related to metabolism and immune response in cecal tonsils	1669:1787	The influence of S1 on transcriptome modulation was demonstrated by a strong activation of the genes taking part in the pathways related to metabolism and immune response in cecal tonsils.
30831211	1	57	theme	probiotics	195:204	arg1	effects					169:175	effects	169:175	effects of prebiotics and probiotics	169:204	Synbiotics are the bioactive compounds that synergistically combine effects of prebiotics and probiotics.
30831211	13	58	theme	compound	2097:2104	arg1	composition					2068:2078	the composition	2064:2078	the composition of the bioactive compound	2064:2104	Obtained results let us conclude that synbiotics delivered in ovo have significant impact on chicken transcriptome and their effect depends on the composition of the bioactive compound.
30831211	5	59	theme	eggs	708:711	arg1	incubation					713:722	eggs incubation	708:722	eggs incubation	708:722	On day 12 of eggs incubation, 5850 eggs of broiler chicken were distributed to experimental groups and injected with synbiotic 1 (S1)- Lactobacillus salivarius with galactooligosaccharides (GOS) or synbiotic 2 (S2)- Lactobacillus plantarum with raffinose family oligosaccharides (RFO).
30831211	13	60	theme	chicken	2014:2020	arg1	transcriptome					2022:2034	chicken transcriptome	2014:2034	chicken transcriptome	2014:2034	Obtained results let us conclude that synbiotics delivered in ovo have significant impact on chicken transcriptome and their effect depends on the composition of the bioactive compound.
30831211	2	61	dep	in	301:302	arg1	ovo					304:306	ovo	304:306	ovo	304:306	In poultry, synbiotics can be used to reprogram animal's intestinal microbiota upon perinatal in ovo injection on day 12 of eggs incubation.
30831211	11	62	theme	genes	1696:1700	arg1	activation					1678:1687	a strong activation	1669:1687	a strong activation of the genes taking part in the pathways related to metabolism and immune response in cecal tonsils	1669:1787	The influence of S1 on transcriptome modulation was demonstrated by a strong activation of the genes taking part in the pathways related to metabolism and immune response in cecal tonsils.
30831211	1	63	theme	bioactive	120:128	arg1	compounds					130:138	the bioactive compounds	116:138	the bioactive compounds that synergistically combine effects of prebiotics and probiotics	116:204	Synbiotics are the bioactive compounds that synergistically combine effects of prebiotics and probiotics.
30831211	1	63	theme	bioactive	120:128	arg1	Synbiotics					101:110	Synbiotics	101:110	Synbiotics	101:110	Synbiotics are the bioactive compounds that synergistically combine effects of prebiotics and probiotics.
30831211	11	64	from	response	1763:1770	arg1	tonsils					1781:1787	cecal tonsils	1775:1787	cecal tonsils	1775:1787	The influence of S1 on transcriptome modulation was demonstrated by a strong activation of the genes taking part in the pathways related to metabolism and immune response in cecal tonsils.
30831211	11	65	theme	transcriptome	1624:1636	arg1	modulation					1638:1647	transcriptome modulation	1624:1647	transcriptome modulation	1624:1647	The influence of S1 on transcriptome modulation was demonstrated by a strong activation of the genes taking part in the pathways related to metabolism and immune response in cecal tonsils.
30831211	7	66	theme	Isolated	1152:1159	arg1	template					1177:1184	a template	1175:1184	a template for the whole-transcriptome analysis using GeneChip Chicken Gene 1.1	1175:1253	Isolated RNA served as a template for the whole-transcriptome analysis using GeneChip Chicken Gene 1.1.
30831211	7	66	theme	Isolated	1152:1159	arg1	RNA					1161:1163	Isolated RNA	1152:1163	Isolated RNA	1152:1163	Isolated RNA served as a template for the whole-transcriptome analysis using GeneChip Chicken Gene 1.1.
30831211	5	67	theme	incubation	713:722	arg1	day					698:700	day 12	698:703	day 12 of eggs incubation	698:722	On day 12 of eggs incubation, 5850 eggs of broiler chicken were distributed to experimental groups and injected with synbiotic 1 (S1)- Lactobacillus salivarius with galactooligosaccharides (GOS) or synbiotic 2 (S2)- Lactobacillus plantarum with raffinose family oligosaccharides (RFO).
30831211	5	68	theme	broiler	738:744	arg1	chicken					746:752	broiler chicken	738:752	broiler chicken	738:752	On day 12 of eggs incubation, 5850 eggs of broiler chicken were distributed to experimental groups and injected with synbiotic 1 (S1)- Lactobacillus salivarius with galactooligosaccharides (GOS) or synbiotic 2 (S2)- Lactobacillus plantarum with raffinose family oligosaccharides (RFO).
30831211	12	69	theme	S2	1790:1791	arg1	injection					1793:1801	S2 injection	1790:1801	S2 injection	1790:1801	S2 injection led to modulation of the gene expression associated with metabolic and developmental signaling pathways in the liver.
30831211	11	70	from	influence	1605:1613	arg1	modulation					1638:1647	transcriptome modulation	1624:1647	transcriptome modulation	1624:1647	The influence of S1 on transcriptome modulation was demonstrated by a strong activation of the genes taking part in the pathways related to metabolism and immune response in cecal tonsils.
30831211	0	71	from	modulation	14:23	arg1	range					75:79	a range	73:79	a range of chicken tissues	73:98	Transcriptome modulation by in ovo delivered Lactobacillus synbiotics in a range of chicken tissues.
30831211	4	72	theme	synbiotics	683:692	arg1	administration					651:664	a single in ovo administration	635:664	a single in ovo administration of two different synbiotics	635:692	The aim of the study was to estimate long-term changes in the chicken transcriptome after a single in ovo administration of two different synbiotics.
30831211	0	73	theme	tissues	92:98	arg1	range					75:79	a range	73:79	a range of chicken tissues	73:98	Transcriptome modulation by in ovo delivered Lactobacillus synbiotics in a range of chicken tissues.
30831211	2	74	from	injection	308:316	arg1	day					321:323	day 12	321:326	day 12 of eggs incubation	321:345	In poultry, synbiotics can be used to reprogram animal's intestinal microbiota upon perinatal in ovo injection on day 12 of eggs incubation.
30831211	3	75	theme	composed	358:365	arg1	synbiotic					367:375	Optimally composed synbiotic	348:375	Optimally composed synbiotic delivered in ovo	348:392	Optimally composed synbiotic delivered in ovo efficiently stimulates the host's intestinal microflora, which in turn exerts beneficial effects on the host and improves its physiological functions.
30831211	13	76	theme	Obtained	1921:1928	arg1	results					1930:1936	Obtained results let us	1921:1943	Obtained results let us	1921:1943	Obtained results let us conclude that synbiotics delivered in ovo have significant impact on chicken transcriptome and their effect depends on the composition of the bioactive compound.
30831211	10	77	located	detected	1495:1502	arg1	tonsils					1513:1519	cecal tonsils	1507:1519	cecal tonsils (160 DEG)	1507:1529	The highest number of Differentially Expressed Genes (DEG) was detected in cecal tonsils (160 DEG) after S1 in ovo injection, and in liver (159 DEG) after S2 injection.
30831211	10	77	located	detected	1495:1502	arg1	DEG					1526:1528	160 DEG	1522:1528	160 DEG	1522:1528	The highest number of Differentially Expressed Genes (DEG) was detected in cecal tonsils (160 DEG) after S1 in ovo injection, and in liver (159 DEG) after S2 injection.
30831211	10	77	located	detected	1495:1502	arg2	number					1444:1449	The highest number	1432:1449	The highest number of Differentially Expressed Genes (DEG)	1432:1489	The highest number of Differentially Expressed Genes (DEG) was detected in cecal tonsils (160 DEG) after S1 in ovo injection, and in liver (159 DEG) after S2 injection.
30831211	3	78	theme	intestinal	428:437	arg1	microflora					439:448	the host's intestinal microflora	417:448	the host's intestinal microflora	417:448	Optimally composed synbiotic delivered in ovo efficiently stimulates the host's intestinal microflora, which in turn exerts beneficial effects on the host and improves its physiological functions.
30831211	0	79	theme	in	28:29	arg1	synbiotics					59:68	in ovo delivered Lactobacillus synbiotics	28:68	in ovo delivered Lactobacillus synbiotics	28:68	Transcriptome modulation by in ovo delivered Lactobacillus synbiotics in a range of chicken tissues.
30831211	7	80	theme	Chicken	1238:1244	arg1	Gene					1246:1249	GeneChip Chicken Gene 1.1	1229:1253	GeneChip Chicken Gene 1.1	1229:1253	Isolated RNA served as a template for the whole-transcriptome analysis using GeneChip Chicken Gene 1.1.
30831211	4	81	theme	study	560:564	arg1	aim					549:551	The aim	545:551	The aim of the study	545:564	The aim of the study was to estimate long-term changes in the chicken transcriptome after a single in ovo administration of two different synbiotics.
30831211	6	82	dep	immunological	1035:1047	arg1	spleen					1068:1073	spleen	1068:1073	spleen	1068:1073	On day 21 post-hatching cockerels were sacrificed and immunological (cecal tonsils and spleen), intestinal (jejunum) and metabolic (liver) tissues were collected (n = 5).
30831211	6	82	dep	immunological	1035:1047	arg1	tonsils					1056:1062	cecal tonsils	1050:1062	cecal tonsils	1050:1062	On day 21 post-hatching cockerels were sacrificed and immunological (cecal tonsils and spleen), intestinal (jejunum) and metabolic (liver) tissues were collected (n = 5).
30831211	0	83	theme	delivered	35:43	arg1	synbiotics					59:68	in ovo delivered Lactobacillus synbiotics	28:68	in ovo delivered Lactobacillus synbiotics	28:68	Transcriptome modulation by in ovo delivered Lactobacillus synbiotics in a range of chicken tissues.
30831211	6	84	theme	post-hatching	991:1003	arg1	cockerels					1005:1013	21 post-hatching cockerels	988:1013	21 post-hatching cockerels	988:1013	On day 21 post-hatching cockerels were sacrificed and immunological (cecal tonsils and spleen), intestinal (jejunum) and metabolic (liver) tissues were collected (n = 5).
30831211	13	85	dep	results	1930:1936	arg1	let					1938:1940	let	1938:1940	Obtained results let us	1921:1943	Obtained results let us conclude that synbiotics delivered in ovo have significant impact on chicken transcriptome and their effect depends on the composition of the bioactive compound.
30831211	9	86	theme	Data	1285:1288	arg1	analysis					1290:1297	Data analysis	1285:1297	Data analysis	1285:1297	Data analysis was performed using Affymetrix Expression Console and Transcriptome Analysis Console software, Venn diagrams, DAVID and CateGOrizer.
30831211	7	87	theme	whole-transcriptome	1194:1212	arg1	analysis					1214:1221	the whole-transcriptome analysis	1190:1221	the whole-transcriptome analysis using GeneChip Chicken Gene 1.1	1190:1253	Isolated RNA served as a template for the whole-transcriptome analysis using GeneChip Chicken Gene 1.1.
30831211	11	88	theme	related	1730:1736	arg1	pathways					1721:1728	the pathways	1717:1728	the pathways related to metabolism and immune response in cecal tonsils	1717:1787	The influence of S1 on transcriptome modulation was demonstrated by a strong activation of the genes taking part in the pathways related to metabolism and immune response in cecal tonsils.
30831211	4	89	dep	in	644:645	arg1	ovo					647:649	ovo	647:649	ovo	647:649	The aim of the study was to estimate long-term changes in the chicken transcriptome after a single in ovo administration of two different synbiotics.
30831211	5	90	theme	family	950:955	arg1	oligosaccharides					957:972	raffinose family oligosaccharides	940:972	raffinose family oligosaccharides	940:972	On day 12 of eggs incubation, 5850 eggs of broiler chicken were distributed to experimental groups and injected with synbiotic 1 (S1)- Lactobacillus salivarius with galactooligosaccharides (GOS) or synbiotic 2 (S2)- Lactobacillus plantarum with raffinose family oligosaccharides (RFO).
30831211	4	91	theme	chicken	607:613	arg1	transcriptome					615:627	the chicken transcriptome	603:627	the chicken transcriptome	603:627	The aim of the study was to estimate long-term changes in the chicken transcriptome after a single in ovo administration of two different synbiotics.
30831211	12	92	theme	developmental	1874:1886	arg1	pathways					1898:1905	metabolic and developmental signaling pathways	1860:1905	metabolic and developmental signaling pathways in the liver	1860:1918	S2 injection led to modulation of the gene expression associated with metabolic and developmental signaling pathways in the liver.
30831211	6	93	dep	intestinal	1077:1086	arg1	jejunum					1089:1095	jejunum	1089:1095	jejunum	1089:1095	On day 21 post-hatching cockerels were sacrificed and immunological (cecal tonsils and spleen), intestinal (jejunum) and metabolic (liver) tissues were collected (n = 5).
30831211	11	94	theme	immune	1756:1761	arg1	response					1763:1770	immune response	1756:1770	immune response	1756:1770	The influence of S1 on transcriptome modulation was demonstrated by a strong activation of the genes taking part in the pathways related to metabolism and immune response in cecal tonsils.
30831211	9	95	theme	Expression	1330:1339	arg1	Analysis					1367:1374	Affymetrix Expression Console and Transcriptome Analysis Console software, Venn diagrams, DAVID and CateGOrizer	1319:1429	Analysis	1367:1374	Data analysis was performed using Affymetrix Expression Console and Transcriptome Analysis Console software, Venn diagrams, DAVID and CateGOrizer.
30904313	7	0	from	values	1561:1566	arg1	increment					1537:1545	The rectal temperature increment	1514:1545	The rectal temperature increment from baseline values in response to LPS	1514:1585	The rectal temperature increment from baseline values in response to LPS did not differ, but cortisol increment was greater and cortisol response area under the curve (AUC) tended to be greater [202 vs. 122 (ng/mL) × 10 h] for REST than CONT.
30904313	2	1	theme	=	549:549	arg1	n					547:547	n = 9	547:551	n = 9	547:551	Starting at 24 ± 3 d in milk, multiparous Holstein cows either received a ration containing 48% straw for 96 h to restrict nutrient intake (REST, n = 8) or were allowed ad libitum intake of a lactation diet (CONT, n = 9).
30904313	8	2	theme	necrosis	1827:1834	arg1	factor-α					1836:1843	tumor necrosis factor-α	1821:1843	tumor necrosis factor-α	1821:1843	No treatment differences were observed in foremilk IL-8, IL-1β, tumor necrosis factor-α, and chemokine (C-X-C motif) ligand 3 concentrations in response to LPS injection.
30904313	6	3	theme	intake	1023:1028	arg1	concentrations					1137:1150	Dry matter intake, milk yield, energy balance, plasma glucose, nonesterified fatty acids (NEFA), and β-hydroxybutyrate (BHB) concentrations	1012:1150	Dry matter intake, milk yield, energy balance, plasma glucose, nonesterified fatty acids (NEFA), and β-hydroxybutyrate (BHB) concentrations	1012:1150	Dry matter intake, milk yield, energy balance, plasma glucose, nonesterified fatty acids (NEFA), and β-hydroxybutyrate (BHB) concentrations did not differ between CONT and REST immediately before nutrient restriction in REST (least squares means at d -1 were 21.8, 39.0 kg/d, -2.5 MJ/d, and 3.78, 0.415, 0.66 mM, respectively) but were significantly altered at 72 h of nutrient restriction (9.8, 28.3 kg/d, -81.6 MJ/d, and 2.77, 1.672, and 2.98 mM, respectively), when the LPS challenge was performed.
30904313	2	4	theme	multiparous	363:373	arg1	cows					384:387	multiparous Holstein cows	363:387	multiparous Holstein cows	363:387	Starting at 24 ± 3 d in milk, multiparous Holstein cows either received a ration containing 48% straw for 96 h to restrict nutrient intake (REST, n = 8) or were allowed ad libitum intake of a lactation diet (CONT, n = 9).
30904313	2	5	from	d	352:352	arg1	milk					357:360	milk	357:360	milk	357:360	Starting at 24 ± 3 d in milk, multiparous Holstein cows either received a ration containing 48% straw for 96 h to restrict nutrient intake (REST, n = 8) or were allowed ad libitum intake of a lactation diet (CONT, n = 9).
30904313	3	6	from	h	564:564	arg1	diet					569:572	diet	569:572	diet	569:572	After 72 h on diet or after an equivalent period for CONT, 50 µg of LPS (Escherichia coli 0111:B4) was injected into one healthy rear mammary quarter to induce an acute inflammation response.
30904313	6	7	theme	Dry	1012:1014	arg1	BHB					1132:1134	BHB	1132:1134	BHB	1132:1134	Dry matter intake, milk yield, energy balance, plasma glucose, nonesterified fatty acids (NEFA), and β-hydroxybutyrate (BHB) concentrations did not differ between CONT and REST immediately before nutrient restriction in REST (least squares means at d -1 were 21.8, 39.0 kg/d, -2.5 MJ/d, and 3.78, 0.415, 0.66 mM, respectively) but were significantly altered at 72 h of nutrient restriction (9.8, 28.3 kg/d, -81.6 MJ/d, and 2.77, 1.672, and 2.98 mM, respectively), when the LPS challenge was performed.
30904313	6	7	theme	Dry	1012:1014	arg1	intake					1023:1028	Dry matter intake	1012:1028	Dry matter intake	1012:1028	Dry matter intake, milk yield, energy balance, plasma glucose, nonesterified fatty acids (NEFA), and β-hydroxybutyrate (BHB) concentrations did not differ between CONT and REST immediately before nutrient restriction in REST (least squares means at d -1 were 21.8, 39.0 kg/d, -2.5 MJ/d, and 3.78, 0.415, 0.66 mM, respectively) but were significantly altered at 72 h of nutrient restriction (9.8, 28.3 kg/d, -81.6 MJ/d, and 2.77, 1.672, and 2.98 mM, respectively), when the LPS challenge was performed.
30904313	8	8	theme	chemokine	1850:1858	arg1	ligand					1874:1879	chemokine (C-X-C motif) ligand 3	1850:1881	chemokine (C-X-C motif) ligand 3	1850:1881	No treatment differences were observed in foremilk IL-8, IL-1β, tumor necrosis factor-α, and chemokine (C-X-C motif) ligand 3 concentrations in response to LPS injection.
30904313	6	9	dep	REST	1232:1235	arg1	means					1252:1256	least squares means	1238:1256	least squares means at d -1 were 21.8, 39.0 kg/d, -2.5 MJ/d, and 3.78, 0.415, 0.66 mM, respectively	1238:1336	Dry matter intake, milk yield, energy balance, plasma glucose, nonesterified fatty acids (NEFA), and β-hydroxybutyrate (BHB) concentrations did not differ between CONT and REST immediately before nutrient restriction in REST (least squares means at d -1 were 21.8, 39.0 kg/d, -2.5 MJ/d, and 3.78, 0.415, 0.66 mM, respectively) but were significantly altered at 72 h of nutrient restriction (9.8, 28.3 kg/d, -81.6 MJ/d, and 2.77, 1.672, and 2.98 mM, respectively), when the LPS challenge was performed.
30904313	4	10	theme	LPS	903:905	arg1	injection					907:915	LPS injection	903:915	LPS injection	903:915	Blood samples were collected weekly until 7 wk of lactation, daily during feed restriction (or control), before and at 1, 2, 4, 6, 10, and 24 h relative to LPS injection.
30904313	6	11	theme	squares	1244:1250	arg1	means					1252:1256	least squares means	1238:1256	least squares means at d -1 were 21.8, 39.0 kg/d, -2.5 MJ/d, and 3.78, 0.415, 0.66 mM, respectively	1238:1336	Dry matter intake, milk yield, energy balance, plasma glucose, nonesterified fatty acids (NEFA), and β-hydroxybutyrate (BHB) concentrations did not differ between CONT and REST immediately before nutrient restriction in REST (least squares means at d -1 were 21.8, 39.0 kg/d, -2.5 MJ/d, and 3.78, 0.415, 0.66 mM, respectively) but were significantly altered at 72 h of nutrient restriction (9.8, 28.3 kg/d, -81.6 MJ/d, and 2.77, 1.672, and 2.98 mM, respectively), when the LPS challenge was performed.
30904313	8	12	theme	LPS	1913:1915	arg1	injection					1917:1925	LPS injection	1913:1925	LPS injection	1913:1925	No treatment differences were observed in foremilk IL-8, IL-1β, tumor necrosis factor-α, and chemokine (C-X-C motif) ligand 3 concentrations in response to LPS injection.
30904313	11	13	theme	smaller	2456:2462	arg1	responses					2438:2446	delayed plasma insulin and glucose responses	2403:2446	delayed plasma insulin and glucose responses to LPS	2403:2453	Nonetheless, nutrient-restricted cows had delayed plasma insulin and glucose responses to LPS, smaller insulin AUC but greater glucose AUC compared with CONT, despite the limited nutrient availability to sustain an inflammation response.
30904313	11	13	theme	smaller	2456:2462	arg1	AUC					2472:2474	smaller insulin AUC	2456:2474	smaller insulin AUC	2456:2474	Nonetheless, nutrient-restricted cows had delayed plasma insulin and glucose responses to LPS, smaller insulin AUC but greater glucose AUC compared with CONT, despite the limited nutrient availability to sustain an inflammation response.
30904313	8	14	theme	C-X-C	1861:1865	arg1	ligand					1874:1879	chemokine (C-X-C motif) ligand 3	1850:1881	chemokine (C-X-C motif) ligand 3	1850:1881	No treatment differences were observed in foremilk IL-8, IL-1β, tumor necrosis factor-α, and chemokine (C-X-C motif) ligand 3 concentrations in response to LPS injection.
30904313	2	15	theme	diet	535:538	arg1	intake					513:518	ad libitum intake	502:518	ad libitum intake of a lactation diet (CONT, n = 9)	502:552	Starting at 24 ± 3 d in milk, multiparous Holstein cows either received a ration containing 48% straw for 96 h to restrict nutrient intake (REST, n = 8) or were allowed ad libitum intake of a lactation diet (CONT, n = 9).
30904313	12	16	theme	early-lactation	2761:2775	arg1	cows					2777:2780	early-lactation cows	2761:2780	early-lactation cows	2761:2780	Undernutrition altered peripheral metabolic responses to an intramammary LPS challenge but had limited effects on selected indicators of inflammation response in early-lactation cows.
30904313	2	17	theme	±	348:348	arg1	d					352:352	24 ± 3 d	345:352	24 ± 3 d in milk	345:360	Starting at 24 ± 3 d in milk, multiparous Holstein cows either received a ration containing 48% straw for 96 h to restrict nutrient intake (REST, n = 8) or were allowed ad libitum intake of a lactation diet (CONT, n = 9).
30904313	1	18	theme	induced	218:224	arg1	undernutrition					226:239	experimentally induced undernutrition	203:239	experimentally induced undernutrition	203:239	The objective was to assess effects of experimentally induced undernutrition on responses to an intramammary lipopolysaccharide (LPS) challenge in early-lactation cows.
30904313	6	19	theme	plasma	1059:1064	arg1	glucose					1066:1072	plasma glucose	1059:1072	plasma glucose	1059:1072	Dry matter intake, milk yield, energy balance, plasma glucose, nonesterified fatty acids (NEFA), and β-hydroxybutyrate (BHB) concentrations did not differ between CONT and REST immediately before nutrient restriction in REST (least squares means at d -1 were 21.8, 39.0 kg/d, -2.5 MJ/d, and 3.78, 0.415, 0.66 mM, respectively) but were significantly altered at 72 h of nutrient restriction (9.8, 28.3 kg/d, -81.6 MJ/d, and 2.77, 1.672, and 2.98 mM, respectively), when the LPS challenge was performed.
30904313	11	20	theme	greater	2480:2486	arg1	AUC					2496:2498	greater glucose AUC	2480:2498	greater glucose AUC	2480:2498	Nonetheless, nutrient-restricted cows had delayed plasma insulin and glucose responses to LPS, smaller insulin AUC but greater glucose AUC compared with CONT, despite the limited nutrient availability to sustain an inflammation response.
30904313	11	20	theme	greater	2480:2486	arg1	responses					2438:2446	delayed plasma insulin and glucose responses	2403:2446	delayed plasma insulin and glucose responses to LPS	2403:2453	Nonetheless, nutrient-restricted cows had delayed plasma insulin and glucose responses to LPS, smaller insulin AUC but greater glucose AUC compared with CONT, despite the limited nutrient availability to sustain an inflammation response.
30904313	4	21	theme	lactation	797:805	arg1	wk					791:792	7 wk	789:792	7 wk of lactation	789:805	Blood samples were collected weekly until 7 wk of lactation, daily during feed restriction (or control), before and at 1, 2, 4, 6, 10, and 24 h relative to LPS injection.
30904313	10	22	theme	Plasma	2137:2142	arg1	glucose					2144:2150	Plasma glucose	2137:2150	Plasma glucose	2137:2150	Plasma glucose, urea, and insulin concentrations increased after the LPS challenge, suggesting establishment of insulin resistance and modifications of glucose metabolism to support acute inflammation in both CONT and REST.
30904313	6	23	theme	balance	1050:1056	arg1	concentrations					1137:1150	Dry matter intake, milk yield, energy balance, plasma glucose, nonesterified fatty acids (NEFA), and β-hydroxybutyrate (BHB) concentrations	1012:1150	Dry matter intake, milk yield, energy balance, plasma glucose, nonesterified fatty acids (NEFA), and β-hydroxybutyrate (BHB) concentrations	1012:1150	Dry matter intake, milk yield, energy balance, plasma glucose, nonesterified fatty acids (NEFA), and β-hydroxybutyrate (BHB) concentrations did not differ between CONT and REST immediately before nutrient restriction in REST (least squares means at d -1 were 21.8, 39.0 kg/d, -2.5 MJ/d, and 3.78, 0.415, 0.66 mM, respectively) but were significantly altered at 72 h of nutrient restriction (9.8, 28.3 kg/d, -81.6 MJ/d, and 2.77, 1.672, and 2.98 mM, respectively), when the LPS challenge was performed.
30904313	7	24	theme	baseline	1552:1559	arg1	values					1561:1566	baseline values	1552:1566	baseline values	1552:1566	The rectal temperature increment from baseline values in response to LPS did not differ, but cortisol increment was greater and cortisol response area under the curve (AUC) tended to be greater [202 vs. 122 (ng/mL) × 10 h] for REST than CONT.
30904313	10	25	from	inflammation	2325:2336	arg1	REST					2355:2358	REST	2355:2358	REST	2355:2358	Plasma glucose, urea, and insulin concentrations increased after the LPS challenge, suggesting establishment of insulin resistance and modifications of glucose metabolism to support acute inflammation in both CONT and REST.
30904313	10	25	from	inflammation	2325:2336	arg1	CONT					2346:2349	CONT	2346:2349	CONT	2346:2349	Plasma glucose, urea, and insulin concentrations increased after the LPS challenge, suggesting establishment of insulin resistance and modifications of glucose metabolism to support acute inflammation in both CONT and REST.
30904313	12	26	theme	response	2749:2756	arg1	indicators					2722:2731	selected indicators	2713:2731	selected indicators of inflammation response in early-lactation cows	2713:2780	Undernutrition altered peripheral metabolic responses to an intramammary LPS challenge but had limited effects on selected indicators of inflammation response in early-lactation cows.
30904313	9	27	theme	milk	1938:1941	arg1	cell					1951:1954	Composite milk somatic cell	1928:1954	Composite milk somatic cell count per milliliter (6.919 × 106 vs. 1.956 × 106 cells/mL)	1928:2014	Composite milk somatic cell count per milliliter (6.919 × 106 vs. 1.956 × 106 cells/mL) and total number of somatic cells secreted in milk per day were greater for REST than CONT during the day following LPS.
30904313	3	28	theme	acute	718:722	arg1	response					737:744	an acute inflammation response	715:744	an acute inflammation response	715:744	After 72 h on diet or after an equivalent period for CONT, 50 µg of LPS (Escherichia coli 0111:B4) was injected into one healthy rear mammary quarter to induce an acute inflammation response.
30904313	2	29	theme	lactation	525:533	arg1	CONT					541:544	CONT	541:544	CONT	541:544	Starting at 24 ± 3 d in milk, multiparous Holstein cows either received a ration containing 48% straw for 96 h to restrict nutrient intake (REST, n = 8) or were allowed ad libitum intake of a lactation diet (CONT, n = 9).
30904313	2	29	theme	lactation	525:533	arg1	diet					535:538	a lactation diet	523:538	a lactation diet (CONT, n = 9)	523:552	Starting at 24 ± 3 d in milk, multiparous Holstein cows either received a ration containing 48% straw for 96 h to restrict nutrient intake (REST, n = 8) or were allowed ad libitum intake of a lactation diet (CONT, n = 9).
30904313	0	30	from	indicators	128:137	arg1	cows					158:161	early-lactation cows	142:161	early-lactation cows	142:161	Undernutrition modified metabolic responses to intramammary lipopolysaccharide but had limited effects on selected inflammation indicators in early-lactation cows.
30904313	8	31	theme	treatment	1760:1768	arg1	differences					1770:1780	No treatment differences	1757:1780	No treatment differences	1757:1780	No treatment differences were observed in foremilk IL-8, IL-1β, tumor necrosis factor-α, and chemokine (C-X-C motif) ligand 3 concentrations in response to LPS injection.
30904313	12	32	contain	had	2690:2692	arg1	Undernutrition					2599:2612	Undernutrition	2599:2612	Undernutrition	2599:2612	Undernutrition altered peripheral metabolic responses to an intramammary LPS challenge but had limited effects on selected indicators of inflammation response in early-lactation cows.
30904313	12	32	contain	had	2690:2692	arg2	effects					2702:2708	limited effects	2694:2708	limited effects	2694:2708	Undernutrition altered peripheral metabolic responses to an intramammary LPS challenge but had limited effects on selected indicators of inflammation response in early-lactation cows.
30904313	6	33	theme	milk	1031:1034	arg1	yield					1036:1040	milk yield	1031:1040	milk yield	1031:1040	Dry matter intake, milk yield, energy balance, plasma glucose, nonesterified fatty acids (NEFA), and β-hydroxybutyrate (BHB) concentrations did not differ between CONT and REST immediately before nutrient restriction in REST (least squares means at d -1 were 21.8, 39.0 kg/d, -2.5 MJ/d, and 3.78, 0.415, 0.66 mM, respectively) but were significantly altered at 72 h of nutrient restriction (9.8, 28.3 kg/d, -81.6 MJ/d, and 2.77, 1.672, and 2.98 mM, respectively), when the LPS challenge was performed.
30904313	9	34	theme	cell	1951:1954	arg1	count					1956:1960	Composite milk somatic cell count	1928:1960	Composite milk somatic cell count per milliliter (6.919 × 106 vs. 1.956 × 106 cells/mL)	1928:2014	Composite milk somatic cell count per milliliter (6.919 × 106 vs. 1.956 × 106 cells/mL) and total number of somatic cells secreted in milk per day were greater for REST than CONT during the day following LPS.
30904313	12	35	theme	selected	2713:2720	arg1	indicators					2722:2731	selected indicators	2713:2731	selected indicators of inflammation response in early-lactation cows	2713:2780	Undernutrition altered peripheral metabolic responses to an intramammary LPS challenge but had limited effects on selected indicators of inflammation response in early-lactation cows.
30904313	1	36	from	effects	192:198	arg1	responses					244:252	responses	244:252	responses to an intramammary lipopolysaccharide (LPS) challenge in early-lactation cows	244:330	The objective was to assess effects of experimentally induced undernutrition on responses to an intramammary lipopolysaccharide (LPS) challenge in early-lactation cows.
30904313	6	37	from	d	1261:1261	arg1	means					1252:1256	least squares means	1238:1256	least squares means at d -1 were 21.8, 39.0 kg/d, -2.5 MJ/d, and 3.78, 0.415, 0.66 mM, respectively	1238:1336	Dry matter intake, milk yield, energy balance, plasma glucose, nonesterified fatty acids (NEFA), and β-hydroxybutyrate (BHB) concentrations did not differ between CONT and REST immediately before nutrient restriction in REST (least squares means at d -1 were 21.8, 39.0 kg/d, -2.5 MJ/d, and 3.78, 0.415, 0.66 mM, respectively) but were significantly altered at 72 h of nutrient restriction (9.8, 28.3 kg/d, -81.6 MJ/d, and 2.77, 1.672, and 2.98 mM, respectively), when the LPS challenge was performed.
30904313	11	38	theme	limited	2532:2538	arg1	availability					2549:2560	the limited nutrient availability	2528:2560	the limited nutrient availability to sustain an inflammation response	2528:2596	Nonetheless, nutrient-restricted cows had delayed plasma insulin and glucose responses to LPS, smaller insulin AUC but greater glucose AUC compared with CONT, despite the limited nutrient availability to sustain an inflammation response.
30904313	6	39	theme	restriction	1390:1400	arg1	h					1376:1376	72 h	1373:1376	72 h of nutrient restriction (9.8, 28.3 kg/d, -81.6 MJ/d, and 2.77, 1.672, and 2.98 mM, respectively)	1373:1473	Dry matter intake, milk yield, energy balance, plasma glucose, nonesterified fatty acids (NEFA), and β-hydroxybutyrate (BHB) concentrations did not differ between CONT and REST immediately before nutrient restriction in REST (least squares means at d -1 were 21.8, 39.0 kg/d, -2.5 MJ/d, and 3.78, 0.415, 0.66 mM, respectively) but were significantly altered at 72 h of nutrient restriction (9.8, 28.3 kg/d, -81.6 MJ/d, and 2.77, 1.672, and 2.98 mM, respectively), when the LPS challenge was performed.
30904313	7	40	theme	cortisol	1642:1649	arg1	area					1660:1663	cortisol response area	1642:1663	cortisol response area under the curve (AUC)	1642:1685	The rectal temperature increment from baseline values in response to LPS did not differ, but cortisol increment was greater and cortisol response area under the curve (AUC) tended to be greater [202 vs. 122 (ng/mL) × 10 h] for REST than CONT.
30904313	5	41	theme	LPS	997:999	arg1	injection					1001:1009	LPS injection	997:1009	LPS injection	997:1009	Foremilk quarter samples were collected before and at 4, 6, 10, and 24 h after LPS injection.
30904313	9	42	theme	×	1984:1984	arg1	milliliter					1966:1975	milliliter	1966:1975	milliliter (6.919 × 106 vs. 1.956 × 106 cells/mL)	1966:2014	Composite milk somatic cell count per milliliter (6.919 × 106 vs. 1.956 × 106 cells/mL) and total number of somatic cells secreted in milk per day were greater for REST than CONT during the day following LPS.
30904313	9	42	theme	×	1984:1984	arg1	cells/mL					2006:2013	6.919 × 106 vs. 1.956 × 106 cells/mL	1978:2013	6.919 × 106 vs. 1.956 × 106 cells/mL	1978:2013	Composite milk somatic cell count per milliliter (6.919 × 106 vs. 1.956 × 106 cells/mL) and total number of somatic cells secreted in milk per day were greater for REST than CONT during the day following LPS.
30904313	11	43	theme	insulin	2418:2424	arg1	AUC					2496:2498	greater glucose AUC	2480:2498	greater glucose AUC	2480:2498	Nonetheless, nutrient-restricted cows had delayed plasma insulin and glucose responses to LPS, smaller insulin AUC but greater glucose AUC compared with CONT, despite the limited nutrient availability to sustain an inflammation response.
30904313	11	43	theme	insulin	2418:2424	arg1	responses					2438:2446	delayed plasma insulin and glucose responses	2403:2446	delayed plasma insulin and glucose responses to LPS	2403:2453	Nonetheless, nutrient-restricted cows had delayed plasma insulin and glucose responses to LPS, smaller insulin AUC but greater glucose AUC compared with CONT, despite the limited nutrient availability to sustain an inflammation response.
30904313	11	43	theme	insulin	2418:2424	arg1	AUC					2472:2474	smaller insulin AUC	2456:2474	smaller insulin AUC	2456:2474	Nonetheless, nutrient-restricted cows had delayed plasma insulin and glucose responses to LPS, smaller insulin AUC but greater glucose AUC compared with CONT, despite the limited nutrient availability to sustain an inflammation response.
30904313	10	44	theme	insulin	2163:2169	arg1	concentrations					2171:2184	Plasma glucose, urea, and insulin concentrations	2137:2184	Plasma glucose, urea, and insulin concentrations	2137:2184	Plasma glucose, urea, and insulin concentrations increased after the LPS challenge, suggesting establishment of insulin resistance and modifications of glucose metabolism to support acute inflammation in both CONT and REST.
30904313	6	45	theme	nutrient	1208:1215	arg1	restriction					1217:1227	nutrient restriction	1208:1227	nutrient restriction in REST (least squares means at d -1 were 21.8, 39.0 kg/d, -2.5 MJ/d, and 3.78, 0.415, 0.66 mM, respectively)	1208:1337	Dry matter intake, milk yield, energy balance, plasma glucose, nonesterified fatty acids (NEFA), and β-hydroxybutyrate (BHB) concentrations did not differ between CONT and REST immediately before nutrient restriction in REST (least squares means at d -1 were 21.8, 39.0 kg/d, -2.5 MJ/d, and 3.78, 0.415, 0.66 mM, respectively) but were significantly altered at 72 h of nutrient restriction (9.8, 28.3 kg/d, -81.6 MJ/d, and 2.77, 1.672, and 2.98 mM, respectively), when the LPS challenge was performed.
30904313	3	46	theme	rear	684:687	arg1	quarter					697:703	one healthy rear mammary quarter	672:703	one healthy rear mammary quarter	672:703	After 72 h on diet or after an equivalent period for CONT, 50 µg of LPS (Escherichia coli 0111:B4) was injected into one healthy rear mammary quarter to induce an acute inflammation response.
30904313	0	47	theme	selected	106:113	arg1	indicators					128:137	selected inflammation indicators	106:137	selected inflammation indicators in early-lactation cows	106:161	Undernutrition modified metabolic responses to intramammary lipopolysaccharide but had limited effects on selected inflammation indicators in early-lactation cows.
30904313	7	48	theme	temperature	1525:1535	arg1	increment					1537:1545	The rectal temperature increment	1514:1545	The rectal temperature increment from baseline values in response to LPS	1514:1585	The rectal temperature increment from baseline values in response to LPS did not differ, but cortisol increment was greater and cortisol response area under the curve (AUC) tended to be greater [202 vs. 122 (ng/mL) × 10 h] for REST than CONT.
30904313	6	49	dep	restriction	1390:1400	arg1	kg/d					1413:1416	9.8, 28.3 kg/d	1403:1416	kg/d	1413:1416	Dry matter intake, milk yield, energy balance, plasma glucose, nonesterified fatty acids (NEFA), and β-hydroxybutyrate (BHB) concentrations did not differ between CONT and REST immediately before nutrient restriction in REST (least squares means at d -1 were 21.8, 39.0 kg/d, -2.5 MJ/d, and 3.78, 0.415, 0.66 mM, respectively) but were significantly altered at 72 h of nutrient restriction (9.8, 28.3 kg/d, -81.6 MJ/d, and 2.77, 1.672, and 2.98 mM, respectively), when the LPS challenge was performed.
30904313	6	49	dep	restriction	1390:1400	arg1	MJ/d					1425:1428	-81.6 MJ/d	1419:1428	-81.6 MJ/d	1419:1428	Dry matter intake, milk yield, energy balance, plasma glucose, nonesterified fatty acids (NEFA), and β-hydroxybutyrate (BHB) concentrations did not differ between CONT and REST immediately before nutrient restriction in REST (least squares means at d -1 were 21.8, 39.0 kg/d, -2.5 MJ/d, and 3.78, 0.415, 0.66 mM, respectively) but were significantly altered at 72 h of nutrient restriction (9.8, 28.3 kg/d, -81.6 MJ/d, and 2.77, 1.672, and 2.98 mM, respectively), when the LPS challenge was performed.
30904313	6	49	dep	restriction	1390:1400	arg1	mM					1457:1458	2.77, 1.672, and 2.98 mM	1435:1458	2.77, 1.672, and 2.98 mM	1435:1458	Dry matter intake, milk yield, energy balance, plasma glucose, nonesterified fatty acids (NEFA), and β-hydroxybutyrate (BHB) concentrations did not differ between CONT and REST immediately before nutrient restriction in REST (least squares means at d -1 were 21.8, 39.0 kg/d, -2.5 MJ/d, and 3.78, 0.415, 0.66 mM, respectively) but were significantly altered at 72 h of nutrient restriction (9.8, 28.3 kg/d, -81.6 MJ/d, and 2.77, 1.672, and 2.98 mM, respectively), when the LPS challenge was performed.
30904313	4	50	theme	feed	821:824	arg1	restriction					826:836	feed restriction	821:836	feed restriction (or control)	821:849	Blood samples were collected weekly until 7 wk of lactation, daily during feed restriction (or control), before and at 1, 2, 4, 6, 10, and 24 h relative to LPS injection.
30904313	4	50	theme	feed	821:824	arg1	control					842:848	control	842:848	control	842:848	Blood samples were collected weekly until 7 wk of lactation, daily during feed restriction (or control), before and at 1, 2, 4, 6, 10, and 24 h relative to LPS injection.
30904313	12	51	theme	limited	2694:2700	arg1	effects					2702:2708	limited effects	2694:2708	limited effects	2694:2708	Undernutrition altered peripheral metabolic responses to an intramammary LPS challenge but had limited effects on selected indicators of inflammation response in early-lactation cows.
30904313	11	52	theme	nutrient	2540:2547	arg1	availability					2549:2560	the limited nutrient availability	2528:2560	the limited nutrient availability to sustain an inflammation response	2528:2596	Nonetheless, nutrient-restricted cows had delayed plasma insulin and glucose responses to LPS, smaller insulin AUC but greater glucose AUC compared with CONT, despite the limited nutrient availability to sustain an inflammation response.
30904313	1	53	theme	early-lactation	311:325	arg1	cows					327:330	early-lactation cows	311:330	early-lactation cows	311:330	The objective was to assess effects of experimentally induced undernutrition on responses to an intramammary lipopolysaccharide (LPS) challenge in early-lactation cows.
30904313	11	54	theme	glucose	2430:2436	arg1	AUC					2496:2498	greater glucose AUC	2480:2498	greater glucose AUC	2480:2498	Nonetheless, nutrient-restricted cows had delayed plasma insulin and glucose responses to LPS, smaller insulin AUC but greater glucose AUC compared with CONT, despite the limited nutrient availability to sustain an inflammation response.
30904313	11	54	theme	glucose	2430:2436	arg1	responses					2438:2446	delayed plasma insulin and glucose responses	2403:2446	delayed plasma insulin and glucose responses to LPS	2403:2453	Nonetheless, nutrient-restricted cows had delayed plasma insulin and glucose responses to LPS, smaller insulin AUC but greater glucose AUC compared with CONT, despite the limited nutrient availability to sustain an inflammation response.
30904313	11	54	theme	glucose	2430:2436	arg1	AUC					2472:2474	smaller insulin AUC	2456:2474	smaller insulin AUC	2456:2474	Nonetheless, nutrient-restricted cows had delayed plasma insulin and glucose responses to LPS, smaller insulin AUC but greater glucose AUC compared with CONT, despite the limited nutrient availability to sustain an inflammation response.
30904313	6	55	from	restriction	1217:1227	arg1	REST					1232:1235	REST	1232:1235	REST (least squares means at d -1 were 21.8, 39.0 kg/d, -2.5 MJ/d, and 3.78, 0.415, 0.66 mM, respectively)	1232:1337	Dry matter intake, milk yield, energy balance, plasma glucose, nonesterified fatty acids (NEFA), and β-hydroxybutyrate (BHB) concentrations did not differ between CONT and REST immediately before nutrient restriction in REST (least squares means at d -1 were 21.8, 39.0 kg/d, -2.5 MJ/d, and 3.78, 0.415, 0.66 mM, respectively) but were significantly altered at 72 h of nutrient restriction (9.8, 28.3 kg/d, -81.6 MJ/d, and 2.77, 1.672, and 2.98 mM, respectively), when the LPS challenge was performed.
30904313	0	56	theme	metabolic	24:32	arg1	responses					34:42	metabolic responses	24:42	metabolic responses to intramammary lipopolysaccharide	24:77	Undernutrition modified metabolic responses to intramammary lipopolysaccharide but had limited effects on selected inflammation indicators in early-lactation cows.
30904313	2	57	theme	%	427:427	arg1	straw					429:433	48% straw	425:433	48% straw	425:433	Starting at 24 ± 3 d in milk, multiparous Holstein cows either received a ration containing 48% straw for 96 h to restrict nutrient intake (REST, n = 8) or were allowed ad libitum intake of a lactation diet (CONT, n = 9).
30904313	5	58	theme	quarter	927:933	arg1	samples					935:941	Foremilk quarter samples	918:941	Foremilk quarter samples	918:941	Foremilk quarter samples were collected before and at 4, 6, 10, and 24 h after LPS injection.
30904313	8	59	theme	IL-8	1808:1811	arg1	concentrations					1883:1896	foremilk IL-8, IL-1β, tumor necrosis factor-α, and chemokine (C-X-C motif) ligand 3 concentrations	1799:1896	foremilk IL-8, IL-1β, tumor necrosis factor-α, and chemokine (C-X-C motif) ligand 3 concentrations in response to LPS injection	1799:1925	No treatment differences were observed in foremilk IL-8, IL-1β, tumor necrosis factor-α, and chemokine (C-X-C motif) ligand 3 concentrations in response to LPS injection.
30904313	10	60	theme	modifications	2272:2284	arg1	establishment					2232:2244	establishment	2232:2244	establishment of insulin resistance and modifications of glucose metabolism to support acute inflammation in both CONT and REST	2232:2358	Plasma glucose, urea, and insulin concentrations increased after the LPS challenge, suggesting establishment of insulin resistance and modifications of glucose metabolism to support acute inflammation in both CONT and REST.
30904313	12	61	from	response	2749:2756	arg1	cows					2777:2780	early-lactation cows	2761:2780	early-lactation cows	2761:2780	Undernutrition altered peripheral metabolic responses to an intramammary LPS challenge but had limited effects on selected indicators of inflammation response in early-lactation cows.
30904313	8	62	theme	IL-1β	1814:1818	arg1	concentrations					1883:1896	foremilk IL-8, IL-1β, tumor necrosis factor-α, and chemokine (C-X-C motif) ligand 3 concentrations	1799:1896	foremilk IL-8, IL-1β, tumor necrosis factor-α, and chemokine (C-X-C motif) ligand 3 concentrations in response to LPS injection	1799:1925	No treatment differences were observed in foremilk IL-8, IL-1β, tumor necrosis factor-α, and chemokine (C-X-C motif) ligand 3 concentrations in response to LPS injection.
30904313	10	63	theme	resistance	2257:2266	arg1	establishment					2232:2244	establishment	2232:2244	establishment of insulin resistance and modifications of glucose metabolism to support acute inflammation in both CONT and REST	2232:2358	Plasma glucose, urea, and insulin concentrations increased after the LPS challenge, suggesting establishment of insulin resistance and modifications of glucose metabolism to support acute inflammation in both CONT and REST.
30904313	3	64	theme	equivalent	586:595	arg1	period					597:602	an equivalent period	583:602	an equivalent period for CONT	583:611	After 72 h on diet or after an equivalent period for CONT, 50 µg of LPS (Escherichia coli 0111:B4) was injected into one healthy rear mammary quarter to induce an acute inflammation response.
30904313	2	65	dep	=	481:481	arg1	REST					473:476	REST	473:476	REST	473:476	Starting at 24 ± 3 d in milk, multiparous Holstein cows either received a ration containing 48% straw for 96 h to restrict nutrient intake (REST, n = 8) or were allowed ad libitum intake of a lactation diet (CONT, n = 9).
30904313	8	66	theme	tumor	1821:1825	arg1	factor-α					1836:1843	tumor necrosis factor-α	1821:1843	tumor necrosis factor-α	1821:1843	No treatment differences were observed in foremilk IL-8, IL-1β, tumor necrosis factor-α, and chemokine (C-X-C motif) ligand 3 concentrations in response to LPS injection.
30904313	12	67	theme	peripheral	2622:2631	arg1	responses					2643:2651	peripheral metabolic responses	2622:2651	peripheral metabolic responses to an intramammary LPS challenge	2622:2684	Undernutrition altered peripheral metabolic responses to an intramammary LPS challenge but had limited effects on selected indicators of inflammation response in early-lactation cows.
30904313	1	68	theme	lipopolysaccharide	273:290	arg1	challenge					298:306	an intramammary lipopolysaccharide (LPS) challenge	257:306	an intramammary lipopolysaccharide (LPS) challenge in early-lactation cows	257:330	The objective was to assess effects of experimentally induced undernutrition on responses to an intramammary lipopolysaccharide (LPS) challenge in early-lactation cows.
30904313	8	69	theme	factor-α	1836:1843	arg1	concentrations					1883:1896	foremilk IL-8, IL-1β, tumor necrosis factor-α, and chemokine (C-X-C motif) ligand 3 concentrations	1799:1896	foremilk IL-8, IL-1β, tumor necrosis factor-α, and chemokine (C-X-C motif) ligand 3 concentrations in response to LPS injection	1799:1925	No treatment differences were observed in foremilk IL-8, IL-1β, tumor necrosis factor-α, and chemokine (C-X-C motif) ligand 3 concentrations in response to LPS injection.
30904313	10	70	theme	glucose	2289:2295	arg1	metabolism					2297:2306	glucose metabolism	2289:2306	glucose metabolism	2289:2306	Plasma glucose, urea, and insulin concentrations increased after the LPS challenge, suggesting establishment of insulin resistance and modifications of glucose metabolism to support acute inflammation in both CONT and REST.
30904313	2	71	theme	Holstein	375:382	arg1	cows					384:387	multiparous Holstein cows	363:387	multiparous Holstein cows	363:387	Starting at 24 ± 3 d in milk, multiparous Holstein cows either received a ration containing 48% straw for 96 h to restrict nutrient intake (REST, n = 8) or were allowed ad libitum intake of a lactation diet (CONT, n = 9).
30904313	11	72	theme	nutrient-restricted	2374:2392	arg1	cows					2394:2397	nutrient-restricted cows	2374:2397	nutrient-restricted cows	2374:2397	Nonetheless, nutrient-restricted cows had delayed plasma insulin and glucose responses to LPS, smaller insulin AUC but greater glucose AUC compared with CONT, despite the limited nutrient availability to sustain an inflammation response.
30904313	11	73	theme	plasma	2411:2416	arg1	insulin					2418:2424	plasma insulin	2411:2424	plasma insulin	2411:2424	Nonetheless, nutrient-restricted cows had delayed plasma insulin and glucose responses to LPS, smaller insulin AUC but greater glucose AUC compared with CONT, despite the limited nutrient availability to sustain an inflammation response.
30904313	6	74	theme	matter	1016:1021	arg1	BHB					1132:1134	BHB	1132:1134	BHB	1132:1134	Dry matter intake, milk yield, energy balance, plasma glucose, nonesterified fatty acids (NEFA), and β-hydroxybutyrate (BHB) concentrations did not differ between CONT and REST immediately before nutrient restriction in REST (least squares means at d -1 were 21.8, 39.0 kg/d, -2.5 MJ/d, and 3.78, 0.415, 0.66 mM, respectively) but were significantly altered at 72 h of nutrient restriction (9.8, 28.3 kg/d, -81.6 MJ/d, and 2.77, 1.672, and 2.98 mM, respectively), when the LPS challenge was performed.
30904313	6	74	theme	matter	1016:1021	arg1	intake					1023:1028	Dry matter intake	1012:1028	Dry matter intake	1012:1028	Dry matter intake, milk yield, energy balance, plasma glucose, nonesterified fatty acids (NEFA), and β-hydroxybutyrate (BHB) concentrations did not differ between CONT and REST immediately before nutrient restriction in REST (least squares means at d -1 were 21.8, 39.0 kg/d, -2.5 MJ/d, and 3.78, 0.415, 0.66 mM, respectively) but were significantly altered at 72 h of nutrient restriction (9.8, 28.3 kg/d, -81.6 MJ/d, and 2.77, 1.672, and 2.98 mM, respectively), when the LPS challenge was performed.
30904313	7	75	theme	cortisol	1607:1614	arg1	increment					1616:1624	cortisol increment	1607:1624	cortisol increment	1607:1624	The rectal temperature increment from baseline values in response to LPS did not differ, but cortisol increment was greater and cortisol response area under the curve (AUC) tended to be greater [202 vs. 122 (ng/mL) × 10 h] for REST than CONT.
30904313	4	76	theme	Blood	747:751	arg1	samples					753:759	Blood samples	747:759	Blood samples	747:759	Blood samples were collected weekly until 7 wk of lactation, daily during feed restriction (or control), before and at 1, 2, 4, 6, 10, and 24 h relative to LPS injection.
30904313	10	77	theme	acute	2319:2323	arg1	inflammation					2325:2336	acute inflammation	2319:2336	acute inflammation in both CONT and REST	2319:2358	Plasma glucose, urea, and insulin concentrations increased after the LPS challenge, suggesting establishment of insulin resistance and modifications of glucose metabolism to support acute inflammation in both CONT and REST.
30904313	3	78	theme	LPS	623:625	arg1	µg					617:618	50 µg	614:618	50 µg of LPS (Escherichia coli 0111:B4)	614:652	After 72 h on diet or after an equivalent period for CONT, 50 µg of LPS (Escherichia coli 0111:B4) was injected into one healthy rear mammary quarter to induce an acute inflammation response.
30904313	8	79	theme	motif	1867:1871	arg1	ligand					1874:1879	chemokine (C-X-C motif) ligand 3	1850:1881	chemokine (C-X-C motif) ligand 3	1850:1881	No treatment differences were observed in foremilk IL-8, IL-1β, tumor necrosis factor-α, and chemokine (C-X-C motif) ligand 3 concentrations in response to LPS injection.
30904313	6	80	theme	LPS	1485:1487	arg1	challenge					1489:1497	the LPS challenge	1481:1497	the LPS challenge	1481:1497	Dry matter intake, milk yield, energy balance, plasma glucose, nonesterified fatty acids (NEFA), and β-hydroxybutyrate (BHB) concentrations did not differ between CONT and REST immediately before nutrient restriction in REST (least squares means at d -1 were 21.8, 39.0 kg/d, -2.5 MJ/d, and 3.78, 0.415, 0.66 mM, respectively) but were significantly altered at 72 h of nutrient restriction (9.8, 28.3 kg/d, -81.6 MJ/d, and 2.77, 1.672, and 2.98 mM, respectively), when the LPS challenge was performed.
30904313	6	81	theme	least	1238:1242	arg1	means					1252:1256	least squares means	1238:1256	least squares means at d -1 were 21.8, 39.0 kg/d, -2.5 MJ/d, and 3.78, 0.415, 0.66 mM, respectively	1238:1336	Dry matter intake, milk yield, energy balance, plasma glucose, nonesterified fatty acids (NEFA), and β-hydroxybutyrate (BHB) concentrations did not differ between CONT and REST immediately before nutrient restriction in REST (least squares means at d -1 were 21.8, 39.0 kg/d, -2.5 MJ/d, and 3.78, 0.415, 0.66 mM, respectively) but were significantly altered at 72 h of nutrient restriction (9.8, 28.3 kg/d, -81.6 MJ/d, and 2.77, 1.672, and 2.98 mM, respectively), when the LPS challenge was performed.
30904313	11	82	theme	insulin	2464:2470	arg1	responses					2438:2446	delayed plasma insulin and glucose responses	2403:2446	delayed plasma insulin and glucose responses to LPS	2403:2453	Nonetheless, nutrient-restricted cows had delayed plasma insulin and glucose responses to LPS, smaller insulin AUC but greater glucose AUC compared with CONT, despite the limited nutrient availability to sustain an inflammation response.
30904313	11	82	theme	insulin	2464:2470	arg1	AUC					2472:2474	smaller insulin AUC	2456:2474	smaller insulin AUC	2456:2474	Nonetheless, nutrient-restricted cows had delayed plasma insulin and glucose responses to LPS, smaller insulin AUC but greater glucose AUC compared with CONT, despite the limited nutrient availability to sustain an inflammation response.
30904313	8	83	theme	ligand	1874:1879	arg1	concentrations					1883:1896	foremilk IL-8, IL-1β, tumor necrosis factor-α, and chemokine (C-X-C motif) ligand 3 concentrations	1799:1896	foremilk IL-8, IL-1β, tumor necrosis factor-α, and chemokine (C-X-C motif) ligand 3 concentrations in response to LPS injection	1799:1925	No treatment differences were observed in foremilk IL-8, IL-1β, tumor necrosis factor-α, and chemokine (C-X-C motif) ligand 3 concentrations in response to LPS injection.
30904313	1	84	from	challenge	298:306	arg1	cows					327:330	early-lactation cows	311:330	early-lactation cows	311:330	The objective was to assess effects of experimentally induced undernutrition on responses to an intramammary lipopolysaccharide (LPS) challenge in early-lactation cows.
30904313	1	85	theme	undernutrition	226:239	arg1	effects					192:198	effects	192:198	effects of experimentally induced undernutrition on responses to an intramammary lipopolysaccharide (LPS) challenge in early-lactation cows	192:330	The objective was to assess effects of experimentally induced undernutrition on responses to an intramammary lipopolysaccharide (LPS) challenge in early-lactation cows.
30904313	10	86	theme	glucose	2144:2150	arg1	concentrations					2171:2184	Plasma glucose, urea, and insulin concentrations	2137:2184	Plasma glucose, urea, and insulin concentrations	2137:2184	Plasma glucose, urea, and insulin concentrations increased after the LPS challenge, suggesting establishment of insulin resistance and modifications of glucose metabolism to support acute inflammation in both CONT and REST.
30904313	3	87	theme	Escherichia	628:638	arg1	0111					645:648	Escherichia coli 0111	628:648	Escherichia coli 0111:B4	628:651	After 72 h on diet or after an equivalent period for CONT, 50 µg of LPS (Escherichia coli 0111:B4) was injected into one healthy rear mammary quarter to induce an acute inflammation response.
30904313	7	88	dep	greater	1700:1706	arg1	h					1734:1734	[202 vs. 122 (ng/mL) × 10 h	1708:1734	[202 vs. 122 (ng/mL) × 10 h	1708:1734	The rectal temperature increment from baseline values in response to LPS did not differ, but cortisol increment was greater and cortisol response area under the curve (AUC) tended to be greater [202 vs. 122 (ng/mL) × 10 h] for REST than CONT.
30904313	7	88	dep	greater	1700:1706	arg1	ng/mL					1722:1726	ng/mL	1722:1726	ng/mL	1722:1726	The rectal temperature increment from baseline values in response to LPS did not differ, but cortisol increment was greater and cortisol response area under the curve (AUC) tended to be greater [202 vs. 122 (ng/mL) × 10 h] for REST than CONT.
30904313	2	89	dep	CONT	541:544	arg1	n					547:547	n = 9	547:551	n = 9	547:551	Starting at 24 ± 3 d in milk, multiparous Holstein cows either received a ration containing 48% straw for 96 h to restrict nutrient intake (REST, n = 8) or were allowed ad libitum intake of a lactation diet (CONT, n = 9).
30904313	2	90	theme	ad	502:503	arg1	intake					513:518	ad libitum intake	502:518	ad libitum intake of a lactation diet (CONT, n = 9)	502:552	Starting at 24 ± 3 d in milk, multiparous Holstein cows either received a ration containing 48% straw for 96 h to restrict nutrient intake (REST, n = 8) or were allowed ad libitum intake of a lactation diet (CONT, n = 9).
30904313	6	91	theme	acids	1095:1099	arg1	concentrations					1137:1150	Dry matter intake, milk yield, energy balance, plasma glucose, nonesterified fatty acids (NEFA), and β-hydroxybutyrate (BHB) concentrations	1012:1150	Dry matter intake, milk yield, energy balance, plasma glucose, nonesterified fatty acids (NEFA), and β-hydroxybutyrate (BHB) concentrations	1012:1150	Dry matter intake, milk yield, energy balance, plasma glucose, nonesterified fatty acids (NEFA), and β-hydroxybutyrate (BHB) concentrations did not differ between CONT and REST immediately before nutrient restriction in REST (least squares means at d -1 were 21.8, 39.0 kg/d, -2.5 MJ/d, and 3.78, 0.415, 0.66 mM, respectively) but were significantly altered at 72 h of nutrient restriction (9.8, 28.3 kg/d, -81.6 MJ/d, and 2.77, 1.672, and 2.98 mM, respectively), when the LPS challenge was performed.
30904313	6	92	theme	energy	1043:1048	arg1	balance					1050:1056	energy balance	1043:1056	energy balance	1043:1056	Dry matter intake, milk yield, energy balance, plasma glucose, nonesterified fatty acids (NEFA), and β-hydroxybutyrate (BHB) concentrations did not differ between CONT and REST immediately before nutrient restriction in REST (least squares means at d -1 were 21.8, 39.0 kg/d, -2.5 MJ/d, and 3.78, 0.415, 0.66 mM, respectively) but were significantly altered at 72 h of nutrient restriction (9.8, 28.3 kg/d, -81.6 MJ/d, and 2.77, 1.672, and 2.98 mM, respectively), when the LPS challenge was performed.
30904313	2	93	contain	containing	414:423	arg1	ration					407:412	a ration	405:412	a ration containing 48% straw	405:433	Starting at 24 ± 3 d in milk, multiparous Holstein cows either received a ration containing 48% straw for 96 h to restrict nutrient intake (REST, n = 8) or were allowed ad libitum intake of a lactation diet (CONT, n = 9).
30904313	2	93	contain	containing	414:423	arg2	straw					429:433	48% straw	425:433	48% straw	425:433	Starting at 24 ± 3 d in milk, multiparous Holstein cows either received a ration containing 48% straw for 96 h to restrict nutrient intake (REST, n = 8) or were allowed ad libitum intake of a lactation diet (CONT, n = 9).
30904313	9	94	theme	Composite	1928:1936	arg1	cell					1951:1954	Composite milk somatic cell	1928:1954	Composite milk somatic cell count per milliliter (6.919 × 106 vs. 1.956 × 106 cells/mL)	1928:2014	Composite milk somatic cell count per milliliter (6.919 × 106 vs. 1.956 × 106 cells/mL) and total number of somatic cells secreted in milk per day were greater for REST than CONT during the day following LPS.
30904313	4	95	dep	relative	891:898	arg1	10					878:879	10	878:879	10	878:879	Blood samples were collected weekly until 7 wk of lactation, daily during feed restriction (or control), before and at 1, 2, 4, 6, 10, and 24 h relative to LPS injection.
30904313	4	95	dep	relative	891:898	arg1	24					886:887	24	886:887	24	886:887	Blood samples were collected weekly until 7 wk of lactation, daily during feed restriction (or control), before and at 1, 2, 4, 6, 10, and 24 h relative to LPS injection.
30904313	5	96	theme	Foremilk	918:925	arg1	samples					935:941	Foremilk quarter samples	918:941	Foremilk quarter samples	918:941	Foremilk quarter samples were collected before and at 4, 6, 10, and 24 h after LPS injection.
30904313	7	97	from	increment	1537:1545	arg1	response					1571:1578	response	1571:1578	response to LPS	1571:1585	The rectal temperature increment from baseline values in response to LPS did not differ, but cortisol increment was greater and cortisol response area under the curve (AUC) tended to be greater [202 vs. 122 (ng/mL) × 10 h] for REST than CONT.
30904313	6	98	theme	glucose	1066:1072	arg1	concentrations					1137:1150	Dry matter intake, milk yield, energy balance, plasma glucose, nonesterified fatty acids (NEFA), and β-hydroxybutyrate (BHB) concentrations	1012:1150	Dry matter intake, milk yield, energy balance, plasma glucose, nonesterified fatty acids (NEFA), and β-hydroxybutyrate (BHB) concentrations	1012:1150	Dry matter intake, milk yield, energy balance, plasma glucose, nonesterified fatty acids (NEFA), and β-hydroxybutyrate (BHB) concentrations did not differ between CONT and REST immediately before nutrient restriction in REST (least squares means at d -1 were 21.8, 39.0 kg/d, -2.5 MJ/d, and 3.78, 0.415, 0.66 mM, respectively) but were significantly altered at 72 h of nutrient restriction (9.8, 28.3 kg/d, -81.6 MJ/d, and 2.77, 1.672, and 2.98 mM, respectively), when the LPS challenge was performed.
30904313	9	99	theme	6.919	1978:1982	arg1	milliliter					1966:1975	milliliter	1966:1975	milliliter (6.919 × 106 vs. 1.956 × 106 cells/mL)	1966:2014	Composite milk somatic cell count per milliliter (6.919 × 106 vs. 1.956 × 106 cells/mL) and total number of somatic cells secreted in milk per day were greater for REST than CONT during the day following LPS.
30904313	9	99	theme	6.919	1978:1982	arg1	cells/mL					2006:2013	6.919 × 106 vs. 1.956 × 106 cells/mL	1978:2013	6.919 × 106 vs. 1.956 × 106 cells/mL	1978:2013	Composite milk somatic cell count per milliliter (6.919 × 106 vs. 1.956 × 106 cells/mL) and total number of somatic cells secreted in milk per day were greater for REST than CONT during the day following LPS.
30904313	12	100	theme	inflammation	2736:2747	arg1	response					2749:2756	inflammation response	2736:2756	inflammation response in early-lactation cows	2736:2780	Undernutrition altered peripheral metabolic responses to an intramammary LPS challenge but had limited effects on selected indicators of inflammation response in early-lactation cows.
30904313	6	101	theme	yield	1036:1040	arg1	concentrations					1137:1150	Dry matter intake, milk yield, energy balance, plasma glucose, nonesterified fatty acids (NEFA), and β-hydroxybutyrate (BHB) concentrations	1012:1150	Dry matter intake, milk yield, energy balance, plasma glucose, nonesterified fatty acids (NEFA), and β-hydroxybutyrate (BHB) concentrations	1012:1150	Dry matter intake, milk yield, energy balance, plasma glucose, nonesterified fatty acids (NEFA), and β-hydroxybutyrate (BHB) concentrations did not differ between CONT and REST immediately before nutrient restriction in REST (least squares means at d -1 were 21.8, 39.0 kg/d, -2.5 MJ/d, and 3.78, 0.415, 0.66 mM, respectively) but were significantly altered at 72 h of nutrient restriction (9.8, 28.3 kg/d, -81.6 MJ/d, and 2.77, 1.672, and 2.98 mM, respectively), when the LPS challenge was performed.
30904313	9	102	theme	somatic	1943:1949	arg1	cell					1951:1954	Composite milk somatic cell	1928:1954	Composite milk somatic cell count per milliliter (6.919 × 106 vs. 1.956 × 106 cells/mL)	1928:2014	Composite milk somatic cell count per milliliter (6.919 × 106 vs. 1.956 × 106 cells/mL) and total number of somatic cells secreted in milk per day were greater for REST than CONT during the day following LPS.
30904313	3	103	theme	inflammation	724:735	arg1	response					737:744	an acute inflammation response	715:744	an acute inflammation response	715:744	After 72 h on diet or after an equivalent period for CONT, 50 µg of LPS (Escherichia coli 0111:B4) was injected into one healthy rear mammary quarter to induce an acute inflammation response.
30904313	10	104	theme	urea	2153:2156	arg1	concentrations					2171:2184	Plasma glucose, urea, and insulin concentrations	2137:2184	Plasma glucose, urea, and insulin concentrations	2137:2184	Plasma glucose, urea, and insulin concentrations increased after the LPS challenge, suggesting establishment of insulin resistance and modifications of glucose metabolism to support acute inflammation in both CONT and REST.
30904313	3	105	theme	healthy	676:682	arg1	quarter					697:703	one healthy rear mammary quarter	672:703	one healthy rear mammary quarter	672:703	After 72 h on diet or after an equivalent period for CONT, 50 µg of LPS (Escherichia coli 0111:B4) was injected into one healthy rear mammary quarter to induce an acute inflammation response.
30904313	0	106	theme	limited	87:93	arg1	effects					95:101	limited effects	87:101	limited effects	87:101	Undernutrition modified metabolic responses to intramammary lipopolysaccharide but had limited effects on selected inflammation indicators in early-lactation cows.
30904313	6	107	theme	fatty	1089:1093	arg1	NEFA					1102:1105	NEFA	1102:1105	NEFA	1102:1105	Dry matter intake, milk yield, energy balance, plasma glucose, nonesterified fatty acids (NEFA), and β-hydroxybutyrate (BHB) concentrations did not differ between CONT and REST immediately before nutrient restriction in REST (least squares means at d -1 were 21.8, 39.0 kg/d, -2.5 MJ/d, and 3.78, 0.415, 0.66 mM, respectively) but were significantly altered at 72 h of nutrient restriction (9.8, 28.3 kg/d, -81.6 MJ/d, and 2.77, 1.672, and 2.98 mM, respectively), when the LPS challenge was performed.
30904313	6	107	theme	fatty	1089:1093	arg1	acids					1095:1099	nonesterified fatty acids	1075:1099	nonesterified fatty acids (NEFA)	1075:1106	Dry matter intake, milk yield, energy balance, plasma glucose, nonesterified fatty acids (NEFA), and β-hydroxybutyrate (BHB) concentrations did not differ between CONT and REST immediately before nutrient restriction in REST (least squares means at d -1 were 21.8, 39.0 kg/d, -2.5 MJ/d, and 3.78, 0.415, 0.66 mM, respectively) but were significantly altered at 72 h of nutrient restriction (9.8, 28.3 kg/d, -81.6 MJ/d, and 2.77, 1.672, and 2.98 mM, respectively), when the LPS challenge was performed.
30904313	4	108	dep	collected	766:774	arg1	before					852:857	before	852:857	before	852:857	Blood samples were collected weekly until 7 wk of lactation, daily during feed restriction (or control), before and at 1, 2, 4, 6, 10, and 24 h relative to LPS injection.
30904313	4	108	dep	collected	766:774	arg1	relative					891:898	relative	891:898	relative	891:898	Blood samples were collected weekly until 7 wk of lactation, daily during feed restriction (or control), before and at 1, 2, 4, 6, 10, and 24 h relative to LPS injection.
30904313	3	109	dep	Escherichia	628:638	arg1	coli					640:643	coli	640:643	coli	640:643	After 72 h on diet or after an equivalent period for CONT, 50 µg of LPS (Escherichia coli 0111:B4) was injected into one healthy rear mammary quarter to induce an acute inflammation response.
30904313	8	110	from	concentrations	1883:1896	arg1	response					1901:1908	response	1901:1908	response to LPS injection	1901:1925	No treatment differences were observed in foremilk IL-8, IL-1β, tumor necrosis factor-α, and chemokine (C-X-C motif) ligand 3 concentrations in response to LPS injection.
30904313	9	111	theme	somatic	2036:2042	arg1	cells					2044:2048	somatic cells	2036:2048	somatic cells secreted in milk per day	2036:2073	Composite milk somatic cell count per milliliter (6.919 × 106 vs. 1.956 × 106 cells/mL) and total number of somatic cells secreted in milk per day were greater for REST than CONT during the day following LPS.
30904313	0	112	theme	early-lactation	142:156	arg1	cows					158:161	early-lactation cows	142:161	early-lactation cows	142:161	Undernutrition modified metabolic responses to intramammary lipopolysaccharide but had limited effects on selected inflammation indicators in early-lactation cows.
30904313	6	113	theme	nutrient	1381:1388	arg1	restriction					1390:1400	nutrient restriction	1381:1400	nutrient restriction (9.8, 28.3 kg/d, -81.6 MJ/d, and 2.77, 1.672, and 2.98 mM, respectively)	1381:1473	Dry matter intake, milk yield, energy balance, plasma glucose, nonesterified fatty acids (NEFA), and β-hydroxybutyrate (BHB) concentrations did not differ between CONT and REST immediately before nutrient restriction in REST (least squares means at d -1 were 21.8, 39.0 kg/d, -2.5 MJ/d, and 3.78, 0.415, 0.66 mM, respectively) but were significantly altered at 72 h of nutrient restriction (9.8, 28.3 kg/d, -81.6 MJ/d, and 2.77, 1.672, and 2.98 mM, respectively), when the LPS challenge was performed.
30904313	7	114	theme	response	1651:1658	arg1	area					1660:1663	cortisol response area	1642:1663	cortisol response area under the curve (AUC)	1642:1685	The rectal temperature increment from baseline values in response to LPS did not differ, but cortisol increment was greater and cortisol response area under the curve (AUC) tended to be greater [202 vs. 122 (ng/mL) × 10 h] for REST than CONT.
30904313	11	115	theme	delayed	2403:2409	arg1	AUC					2496:2498	greater glucose AUC	2480:2498	greater glucose AUC	2480:2498	Nonetheless, nutrient-restricted cows had delayed plasma insulin and glucose responses to LPS, smaller insulin AUC but greater glucose AUC compared with CONT, despite the limited nutrient availability to sustain an inflammation response.
30904313	11	115	theme	delayed	2403:2409	arg1	responses					2438:2446	delayed plasma insulin and glucose responses	2403:2446	delayed plasma insulin and glucose responses to LPS	2403:2453	Nonetheless, nutrient-restricted cows had delayed plasma insulin and glucose responses to LPS, smaller insulin AUC but greater glucose AUC compared with CONT, despite the limited nutrient availability to sustain an inflammation response.
30904313	11	115	theme	delayed	2403:2409	arg1	AUC					2472:2474	smaller insulin AUC	2456:2474	smaller insulin AUC	2456:2474	Nonetheless, nutrient-restricted cows had delayed plasma insulin and glucose responses to LPS, smaller insulin AUC but greater glucose AUC compared with CONT, despite the limited nutrient availability to sustain an inflammation response.
30904313	12	116	theme	LPS	2672:2674	arg1	challenge					2676:2684	an intramammary LPS challenge	2656:2684	an intramammary LPS challenge	2656:2684	Undernutrition altered peripheral metabolic responses to an intramammary LPS challenge but had limited effects on selected indicators of inflammation response in early-lactation cows.
30904313	0	117	theme	inflammation	115:126	arg1	indicators					128:137	selected inflammation indicators	106:137	selected inflammation indicators in early-lactation cows	106:161	Undernutrition modified metabolic responses to intramammary lipopolysaccharide but had limited effects on selected inflammation indicators in early-lactation cows.
30904313	3	118	dep	0111	645:648	arg1	B4					650:651	B4	650:651	Escherichia coli 0111:B4	628:651	After 72 h on diet or after an equivalent period for CONT, 50 µg of LPS (Escherichia coli 0111:B4) was injected into one healthy rear mammary quarter to induce an acute inflammation response.
30904313	7	119	theme	rectal	1518:1523	arg1	temperature					1525:1535	The rectal temperature	1514:1535	The rectal temperature increment from baseline values in response to LPS	1514:1585	The rectal temperature increment from baseline values in response to LPS did not differ, but cortisol increment was greater and cortisol response area under the curve (AUC) tended to be greater [202 vs. 122 (ng/mL) × 10 h] for REST than CONT.
30904313	3	120	theme	mammary	689:695	arg1	quarter					697:703	one healthy rear mammary quarter	672:703	one healthy rear mammary quarter	672:703	After 72 h on diet or after an equivalent period for CONT, 50 µg of LPS (Escherichia coli 0111:B4) was injected into one healthy rear mammary quarter to induce an acute inflammation response.
30904313	6	121	theme	nonesterified	1075:1087	arg1	NEFA					1102:1105	NEFA	1102:1105	NEFA	1102:1105	Dry matter intake, milk yield, energy balance, plasma glucose, nonesterified fatty acids (NEFA), and β-hydroxybutyrate (BHB) concentrations did not differ between CONT and REST immediately before nutrient restriction in REST (least squares means at d -1 were 21.8, 39.0 kg/d, -2.5 MJ/d, and 3.78, 0.415, 0.66 mM, respectively) but were significantly altered at 72 h of nutrient restriction (9.8, 28.3 kg/d, -81.6 MJ/d, and 2.77, 1.672, and 2.98 mM, respectively), when the LPS challenge was performed.
30904313	6	121	theme	nonesterified	1075:1087	arg1	acids					1095:1099	nonesterified fatty acids	1075:1099	nonesterified fatty acids (NEFA)	1075:1106	Dry matter intake, milk yield, energy balance, plasma glucose, nonesterified fatty acids (NEFA), and β-hydroxybutyrate (BHB) concentrations did not differ between CONT and REST immediately before nutrient restriction in REST (least squares means at d -1 were 21.8, 39.0 kg/d, -2.5 MJ/d, and 3.78, 0.415, 0.66 mM, respectively) but were significantly altered at 72 h of nutrient restriction (9.8, 28.3 kg/d, -81.6 MJ/d, and 2.77, 1.672, and 2.98 mM, respectively), when the LPS challenge was performed.
30904313	2	122	dep	h	442:442	arg1	restrict					447:454	restrict	447:454	to restrict nutrient intake	444:470	Starting at 24 ± 3 d in milk, multiparous Holstein cows either received a ration containing 48% straw for 96 h to restrict nutrient intake (REST, n = 8) or were allowed ad libitum intake of a lactation diet (CONT, n = 9).
30904313	10	123	theme	LPS	2206:2208	arg1	challenge					2210:2218	the LPS challenge	2202:2218	the LPS challenge	2202:2218	Plasma glucose, urea, and insulin concentrations increased after the LPS challenge, suggesting establishment of insulin resistance and modifications of glucose metabolism to support acute inflammation in both CONT and REST.
30904313	2	124	theme	libitum	505:511	arg1	intake					513:518	ad libitum intake	502:518	ad libitum intake of a lactation diet (CONT, n = 9)	502:552	Starting at 24 ± 3 d in milk, multiparous Holstein cows either received a ration containing 48% straw for 96 h to restrict nutrient intake (REST, n = 8) or were allowed ad libitum intake of a lactation diet (CONT, n = 9).
30904313	10	125	theme	insulin	2249:2255	arg1	resistance					2257:2266	insulin resistance	2249:2266	insulin resistance	2249:2266	Plasma glucose, urea, and insulin concentrations increased after the LPS challenge, suggesting establishment of insulin resistance and modifications of glucose metabolism to support acute inflammation in both CONT and REST.
30904313	12	126	from	cows	2777:2780	arg1	indicators					2722:2731	selected indicators	2713:2731	selected indicators of inflammation response in early-lactation cows	2713:2780	Undernutrition altered peripheral metabolic responses to an intramammary LPS challenge but had limited effects on selected indicators of inflammation response in early-lactation cows.
30904313	11	127	contain	had	2399:2401	arg2	AUC					2496:2498	greater glucose AUC	2480:2498	greater glucose AUC	2480:2498	Nonetheless, nutrient-restricted cows had delayed plasma insulin and glucose responses to LPS, smaller insulin AUC but greater glucose AUC compared with CONT, despite the limited nutrient availability to sustain an inflammation response.
30904313	11	127	contain	had	2399:2401	arg2	responses					2438:2446	delayed plasma insulin and glucose responses	2403:2446	delayed plasma insulin and glucose responses to LPS	2403:2453	Nonetheless, nutrient-restricted cows had delayed plasma insulin and glucose responses to LPS, smaller insulin AUC but greater glucose AUC compared with CONT, despite the limited nutrient availability to sustain an inflammation response.
30904313	11	127	contain	had	2399:2401	arg2	AUC					2472:2474	smaller insulin AUC	2456:2474	smaller insulin AUC	2456:2474	Nonetheless, nutrient-restricted cows had delayed plasma insulin and glucose responses to LPS, smaller insulin AUC but greater glucose AUC compared with CONT, despite the limited nutrient availability to sustain an inflammation response.
30904313	11	127	contain	had	2399:2401	arg1	cows					2394:2397	nutrient-restricted cows	2374:2397	nutrient-restricted cows	2374:2397	Nonetheless, nutrient-restricted cows had delayed plasma insulin and glucose responses to LPS, smaller insulin AUC but greater glucose AUC compared with CONT, despite the limited nutrient availability to sustain an inflammation response.
30904313	0	128	theme	intramammary	47:58	arg1	lipopolysaccharide					60:77	intramammary lipopolysaccharide	47:77	intramammary lipopolysaccharide	47:77	Undernutrition modified metabolic responses to intramammary lipopolysaccharide but had limited effects on selected inflammation indicators in early-lactation cows.
30904313	11	129	theme	inflammation	2576:2587	arg1	response					2589:2596	an inflammation response	2573:2596	an inflammation response	2573:2596	Nonetheless, nutrient-restricted cows had delayed plasma insulin and glucose responses to LPS, smaller insulin AUC but greater glucose AUC compared with CONT, despite the limited nutrient availability to sustain an inflammation response.
30904313	11	130	theme	glucose	2488:2494	arg1	AUC					2496:2498	greater glucose AUC	2480:2498	greater glucose AUC	2480:2498	Nonetheless, nutrient-restricted cows had delayed plasma insulin and glucose responses to LPS, smaller insulin AUC but greater glucose AUC compared with CONT, despite the limited nutrient availability to sustain an inflammation response.
30904313	11	130	theme	glucose	2488:2494	arg1	responses					2438:2446	delayed plasma insulin and glucose responses	2403:2446	delayed plasma insulin and glucose responses to LPS	2403:2453	Nonetheless, nutrient-restricted cows had delayed plasma insulin and glucose responses to LPS, smaller insulin AUC but greater glucose AUC compared with CONT, despite the limited nutrient availability to sustain an inflammation response.
30904313	12	131	theme	intramammary	2659:2670	arg1	challenge					2676:2684	an intramammary LPS challenge	2656:2684	an intramammary LPS challenge	2656:2684	Undernutrition altered peripheral metabolic responses to an intramammary LPS challenge but had limited effects on selected indicators of inflammation response in early-lactation cows.
30904313	2	132	theme	48	425:426	arg1	%					427:427	%	427:427	%	427:427	Starting at 24 ± 3 d in milk, multiparous Holstein cows either received a ration containing 48% straw for 96 h to restrict nutrient intake (REST, n = 8) or were allowed ad libitum intake of a lactation diet (CONT, n = 9).
30904313	6	133	theme	β-hydroxybutyrate	1113:1129	arg1	concentrations					1137:1150	Dry matter intake, milk yield, energy balance, plasma glucose, nonesterified fatty acids (NEFA), and β-hydroxybutyrate (BHB) concentrations	1012:1150	Dry matter intake, milk yield, energy balance, plasma glucose, nonesterified fatty acids (NEFA), and β-hydroxybutyrate (BHB) concentrations	1012:1150	Dry matter intake, milk yield, energy balance, plasma glucose, nonesterified fatty acids (NEFA), and β-hydroxybutyrate (BHB) concentrations did not differ between CONT and REST immediately before nutrient restriction in REST (least squares means at d -1 were 21.8, 39.0 kg/d, -2.5 MJ/d, and 3.78, 0.415, 0.66 mM, respectively) but were significantly altered at 72 h of nutrient restriction (9.8, 28.3 kg/d, -81.6 MJ/d, and 2.77, 1.672, and 2.98 mM, respectively), when the LPS challenge was performed.
30904313	9	134	theme	total	2020:2024	arg1	number					2026:2031	total number	2020:2031	total number of somatic cells secreted in milk per day	2020:2073	Composite milk somatic cell count per milliliter (6.919 × 106 vs. 1.956 × 106 cells/mL) and total number of somatic cells secreted in milk per day were greater for REST than CONT during the day following LPS.
30904313	8	135	theme	foremilk	1799:1806	arg1	IL-8					1808:1811	foremilk IL-8	1799:1811	foremilk IL-8	1799:1811	No treatment differences were observed in foremilk IL-8, IL-1β, tumor necrosis factor-α, and chemokine (C-X-C motif) ligand 3 concentrations in response to LPS injection.
30904313	2	136	theme	nutrient	456:463	arg1	intake					465:470	nutrient intake	456:470	nutrient intake	456:470	Starting at 24 ± 3 d in milk, multiparous Holstein cows either received a ration containing 48% straw for 96 h to restrict nutrient intake (REST, n = 8) or were allowed ad libitum intake of a lactation diet (CONT, n = 9).
30904313	8	137	located	observed	1787:1794	arg1	concentrations					1883:1896	foremilk IL-8, IL-1β, tumor necrosis factor-α, and chemokine (C-X-C motif) ligand 3 concentrations	1799:1896	foremilk IL-8, IL-1β, tumor necrosis factor-α, and chemokine (C-X-C motif) ligand 3 concentrations in response to LPS injection	1799:1925	No treatment differences were observed in foremilk IL-8, IL-1β, tumor necrosis factor-α, and chemokine (C-X-C motif) ligand 3 concentrations in response to LPS injection.
30904313	8	137	located	observed	1787:1794	arg2	differences					1770:1780	No treatment differences	1757:1780	No treatment differences	1757:1780	No treatment differences were observed in foremilk IL-8, IL-1β, tumor necrosis factor-α, and chemokine (C-X-C motif) ligand 3 concentrations in response to LPS injection.
30904313	3	138	dep	LPS	623:625	arg1	0111					645:648	Escherichia coli 0111	628:648	Escherichia coli 0111:B4	628:651	After 72 h on diet or after an equivalent period for CONT, 50 µg of LPS (Escherichia coli 0111:B4) was injected into one healthy rear mammary quarter to induce an acute inflammation response.
30904313	12	139	from	indicators	2722:2731	arg1	cows					2777:2780	early-lactation cows	2761:2780	early-lactation cows	2761:2780	Undernutrition altered peripheral metabolic responses to an intramammary LPS challenge but had limited effects on selected indicators of inflammation response in early-lactation cows.
30904313	12	140	theme	metabolic	2633:2641	arg1	responses					2643:2651	peripheral metabolic responses	2622:2651	peripheral metabolic responses to an intramammary LPS challenge	2622:2684	Undernutrition altered peripheral metabolic responses to an intramammary LPS challenge but had limited effects on selected indicators of inflammation response in early-lactation cows.
30904313	7	141	theme	×	1729:1729	arg1	h					1734:1734	[202 vs. 122 (ng/mL) × 10 h	1708:1734	[202 vs. 122 (ng/mL) × 10 h	1708:1734	The rectal temperature increment from baseline values in response to LPS did not differ, but cortisol increment was greater and cortisol response area under the curve (AUC) tended to be greater [202 vs. 122 (ng/mL) × 10 h] for REST than CONT.
30904313	7	141	theme	×	1729:1729	arg1	ng/mL					1722:1726	ng/mL	1722:1726	ng/mL	1722:1726	The rectal temperature increment from baseline values in response to LPS did not differ, but cortisol increment was greater and cortisol response area under the curve (AUC) tended to be greater [202 vs. 122 (ng/mL) × 10 h] for REST than CONT.
30904313	1	142	theme	intramammary	260:271	arg1	lipopolysaccharide					273:290	intramammary lipopolysaccharide	260:290	an intramammary lipopolysaccharide (LPS) challenge in early-lactation cows	257:330	The objective was to assess effects of experimentally induced undernutrition on responses to an intramammary lipopolysaccharide (LPS) challenge in early-lactation cows.
30904313	1	142	theme	intramammary	260:271	arg1	LPS					293:295	LPS	293:295	LPS	293:295	The objective was to assess effects of experimentally induced undernutrition on responses to an intramammary lipopolysaccharide (LPS) challenge in early-lactation cows.
30904313	9	143	theme	cells	2044:2048	arg1	number					2026:2031	total number	2020:2031	total number of somatic cells secreted in milk per day	2020:2073	Composite milk somatic cell count per milliliter (6.919 × 106 vs. 1.956 × 106 cells/mL) and total number of somatic cells secreted in milk per day were greater for REST than CONT during the day following LPS.
30904313	9	143	theme	cells	2044:2048	arg1	count					1956:1960	Composite milk somatic cell count	1928:1960	Composite milk somatic cell count per milliliter (6.919 × 106 vs. 1.956 × 106 cells/mL)	1928:2014	Composite milk somatic cell count per milliliter (6.919 × 106 vs. 1.956 × 106 cells/mL) and total number of somatic cells secreted in milk per day were greater for REST than CONT during the day following LPS.
30904313	10	144	theme	metabolism	2297:2306	arg1	modifications					2272:2284	modifications	2272:2284	modifications of glucose metabolism	2272:2306	Plasma glucose, urea, and insulin concentrations increased after the LPS challenge, suggesting establishment of insulin resistance and modifications of glucose metabolism to support acute inflammation in both CONT and REST.
30904313	10	144	theme	metabolism	2297:2306	arg1	resistance					2257:2266	insulin resistance	2249:2266	insulin resistance	2249:2266	Plasma glucose, urea, and insulin concentrations increased after the LPS challenge, suggesting establishment of insulin resistance and modifications of glucose metabolism to support acute inflammation in both CONT and REST.
30904313	0	145	contain	had	83:85	arg1	Undernutrition					0:13	Undernutrition	0:13	Undernutrition	0:13	Undernutrition modified metabolic responses to intramammary lipopolysaccharide but had limited effects on selected inflammation indicators in early-lactation cows.
30904313	0	145	contain	had	83:85	arg2	effects					95:101	limited effects	87:101	limited effects	87:101	Undernutrition modified metabolic responses to intramammary lipopolysaccharide but had limited effects on selected inflammation indicators in early-lactation cows.
30322418	13	0	theme	varying	2681:2687	arg1	level					2689:2693	varying level	2681:2693	varying level	2681:2693	Overall, results suggest that the varying level and source of dietary trace minerals do not have significant short-term effects on oxidative metabolism indices and clinical responses to intramammary LPS challenge in midlactation cows.
30322418	3	1	theme	Council	664:670	arg1	requirements					678:689	National Research Council (NRC) requirements	646:689	National Research Council (NRC) requirements	646:689	Cows were fed a basal diet meeting National Research Council (NRC) requirements except for no added zinc (Zn), copper (Cu) or manganese (Mn).
30322418	9	2	dep	Escherichia	2009:2019	arg1	coli					2021:2024	coli	2021:2024	coli	2021:2024	Plasma immunoglobulin G level was higher in cows supplemented with organic trace minerals over the entire treatment period; responses assessed as differences of before and after Escherichia coli J5 bacterin vaccination at the end of week 2 of treatment period were not significant.
30322418	2	3	theme	lipopolysaccharide	575:592	arg1	challenge					600:608	intramammary lipopolysaccharide (LPS) challenge	562:608	intramammary lipopolysaccharide (LPS) challenge	562:608	Multiparous Holstein cows (n=48) beginning at 60 to 140 days in milk were utilized to determine the effects of trace mineral amount and source on aspects of oxidative metabolism and responses to intramammary lipopolysaccharide (LPS) challenge.
30322418	1	4	with	source	329:334	arg1	outcomes					341:348	outcomes	341:348	outcomes in dairy cattle	341:364	Trace minerals have important roles in immune function and oxidative metabolism; however, little is known about the relationships between supplementation level and source with outcomes in dairy cattle.
30322418	4	5	theme	trace	1312:1316	arg1	supplements					1326:1336	organic trace mineral supplements	1304:1336	organic trace mineral supplements only	1304:1341	After a 4-week preliminary period, cows were assigned to one of four topdress treatments in a randomized complete block design with a 2×2 factorial arrangement of treatments: (1) NRC inorganic (NRC levels using inorganic (sulfate-based) trace mineral supplements only); (2) NRC organic (NRC levels using organic trace mineral supplements (metals chelated to 2-hydroxy-4-(methythio)-butanoic acid); (3) commercial inorganic (approximately 2×NRC levels using inorganic trace mineral supplements only; and (4) commercial organic (commercial levels using organic trace mineral supplements only).
30322418	4	6	dep	NRC	1027:1029	arg1	2					1024:1024	2	1024:1024	2	1024:1024	After a 4-week preliminary period, cows were assigned to one of four topdress treatments in a randomized complete block design with a 2×2 factorial arrangement of treatments: (1) NRC inorganic (NRC levels using inorganic (sulfate-based) trace mineral supplements only); (2) NRC organic (NRC levels using organic trace mineral supplements (metals chelated to 2-hydroxy-4-(methythio)-butanoic acid); (3) commercial inorganic (approximately 2×NRC levels using inorganic trace mineral supplements only; and (4) commercial organic (commercial levels using organic trace mineral supplements only).
30322418	10	7	theme	intramammary	2139:2150	arg1	challenge					2156:2164	an intramammary LPS challenge	2136:2164	an intramammary LPS challenge	2136:2164	Cows were administered an intramammary LPS challenge during week 5; during week 6 cows fed commercial levels of Zn, Cu and Mn tended to have higher plasma TAC and cows fed organic sources had decreased plasma TBARS.
30322418	7	8	theme	total	1625:1629	arg1	TAC					1653:1655	TAC	1653:1655	TAC	1653:1655	Activities of super oxide dismutase and glutathione peroxidase in erythrocyte lysate and concentrations of thiobarbituric acid reactive substances (TBARS) and total antioxidant capacity (TAC) in plasma were measured as indices of oxidative metabolism.
30322418	7	8	theme	total	1625:1629	arg1	capacity					1643:1650	total antioxidant capacity	1625:1650	total antioxidant capacity (TAC)	1625:1656	Activities of super oxide dismutase and glutathione peroxidase in erythrocyte lysate and concentrations of thiobarbituric acid reactive substances (TBARS) and total antioxidant capacity (TAC) in plasma were measured as indices of oxidative metabolism.
30322418	4	9	theme	preliminary	768:778	arg1	period					780:785	a 4-week preliminary period	759:785	a 4-week preliminary period	759:785	After a 4-week preliminary period, cows were assigned to one of four topdress treatments in a randomized complete block design with a 2×2 factorial arrangement of treatments: (1) NRC inorganic (NRC levels using inorganic (sulfate-based) trace mineral supplements only); (2) NRC organic (NRC levels using organic trace mineral supplements (metals chelated to 2-hydroxy-4-(methythio)-butanoic acid); (3) commercial inorganic (approximately 2×NRC levels using inorganic trace mineral supplements only; and (4) commercial organic (commercial levels using organic trace mineral supplements only).
30322418	11	10	theme	trace	2501:2505	arg1	level					2515:2519	trace mineral level	2501:2519	trace mineral level	2501:2519	After the LPS challenge, the extent and pattern of response of plasma cortisol concentrations and clinical indices (rectal temperature and heart rate) were not affected by trace mineral level and source.
30322418	4	11	theme	mineral	1226:1232	arg1	supplements					1234:1244	inorganic trace mineral supplements	1210:1244	inorganic trace mineral supplements only	1210:1249	After a 4-week preliminary period, cows were assigned to one of four topdress treatments in a randomized complete block design with a 2×2 factorial arrangement of treatments: (1) NRC inorganic (NRC levels using inorganic (sulfate-based) trace mineral supplements only); (2) NRC organic (NRC levels using organic trace mineral supplements (metals chelated to 2-hydroxy-4-(methythio)-butanoic acid); (3) commercial inorganic (approximately 2×NRC levels using inorganic trace mineral supplements only; and (4) commercial organic (commercial levels using organic trace mineral supplements only).
30322418	13	12	from	challenge	2850:2858	arg1	cows					2876:2879	midlactation cows	2863:2879	midlactation cows	2863:2879	Overall, results suggest that the varying level and source of dietary trace minerals do not have significant short-term effects on oxidative metabolism indices and clinical responses to intramammary LPS challenge in midlactation cows.
30322418	2	13	theme	oxidative	524:532	arg1	metabolism					534:543	oxidative metabolism	524:543	oxidative metabolism	524:543	Multiparous Holstein cows (n=48) beginning at 60 to 140 days in milk were utilized to determine the effects of trace mineral amount and source on aspects of oxidative metabolism and responses to intramammary lipopolysaccharide (LPS) challenge.
30322418	4	14	theme	sulfate-based	975:987	arg1	supplements					1004:1014	inorganic (sulfate-based) trace mineral supplements	964:1014	inorganic (sulfate-based) trace mineral supplements only	964:1019	After a 4-week preliminary period, cows were assigned to one of four topdress treatments in a randomized complete block design with a 2×2 factorial arrangement of treatments: (1) NRC inorganic (NRC levels using inorganic (sulfate-based) trace mineral supplements only); (2) NRC organic (NRC levels using organic trace mineral supplements (metals chelated to 2-hydroxy-4-(methythio)-butanoic acid); (3) commercial inorganic (approximately 2×NRC levels using inorganic trace mineral supplements only; and (4) commercial organic (commercial levels using organic trace mineral supplements only).
30322418	10	15	theme	decreased	2305:2313	arg1	TBARS					2322:2326	decreased plasma TBARS	2305:2326	decreased plasma TBARS	2305:2326	Cows were administered an intramammary LPS challenge during week 5; during week 6 cows fed commercial levels of Zn, Cu and Mn tended to have higher plasma TAC and cows fed organic sources had decreased plasma TBARS.
30322418	3	16	theme	added	705:709	arg1	Zn					717:718	Zn	717:718	Zn	717:718	Cows were fed a basal diet meeting National Research Council (NRC) requirements except for no added zinc (Zn), copper (Cu) or manganese (Mn).
30322418	3	16	theme	added	705:709	arg1	zinc					711:714	no added zinc	702:714	no added zinc (Zn)	702:719	Cows were fed a basal diet meeting National Research Council (NRC) requirements except for no added zinc (Zn), copper (Cu) or manganese (Mn).
30322418	0	17	theme	lipopolysaccharide	108:125	arg1	challenge					127:135	intramammary lipopolysaccharide challenge	95:135	intramammary lipopolysaccharide challenge in midlactation dairy cows	95:162	Effects of trace mineral amount and source on aspects of oxidative metabolism and responses to intramammary lipopolysaccharide challenge in midlactation dairy cows.
30322418	1	18	theme	immune	204:209	arg1	function					211:218	immune function	204:218	immune function	204:218	Trace minerals have important roles in immune function and oxidative metabolism; however, little is known about the relationships between supplementation level and source with outcomes in dairy cattle.
30322418	13	19	theme	midlactation	2863:2874	arg1	cows					2876:2879	midlactation cows	2863:2879	midlactation cows	2863:2879	Overall, results suggest that the varying level and source of dietary trace minerals do not have significant short-term effects on oxidative metabolism indices and clinical responses to intramammary LPS challenge in midlactation cows.
30322418	4	20	theme	trace	990:994	arg1	supplements					1004:1014	inorganic (sulfate-based) trace mineral supplements	964:1014	inorganic (sulfate-based) trace mineral supplements only	964:1019	After a 4-week preliminary period, cows were assigned to one of four topdress treatments in a randomized complete block design with a 2×2 factorial arrangement of treatments: (1) NRC inorganic (NRC levels using inorganic (sulfate-based) trace mineral supplements only); (2) NRC organic (NRC levels using organic trace mineral supplements (metals chelated to 2-hydroxy-4-(methythio)-butanoic acid); (3) commercial inorganic (approximately 2×NRC levels using inorganic trace mineral supplements only; and (4) commercial organic (commercial levels using organic trace mineral supplements only).
30322418	9	21	theme	bacterin	2029:2036	arg1	vaccination					2038:2048	Escherichia coli J5 bacterin vaccination	2009:2048	Escherichia coli J5 bacterin vaccination	2009:2048	Plasma immunoglobulin G level was higher in cows supplemented with organic trace minerals over the entire treatment period; responses assessed as differences of before and after Escherichia coli J5 bacterin vaccination at the end of week 2 of treatment period were not significant.
30322418	4	22	theme	inorganic	1210:1218	arg1	supplements					1234:1244	inorganic trace mineral supplements	1210:1244	inorganic trace mineral supplements only	1210:1249	After a 4-week preliminary period, cows were assigned to one of four topdress treatments in a randomized complete block design with a 2×2 factorial arrangement of treatments: (1) NRC inorganic (NRC levels using inorganic (sulfate-based) trace mineral supplements only); (2) NRC organic (NRC levels using organic trace mineral supplements (metals chelated to 2-hydroxy-4-(methythio)-butanoic acid); (3) commercial inorganic (approximately 2×NRC levels using inorganic trace mineral supplements only; and (4) commercial organic (commercial levels using organic trace mineral supplements only).
30322418	0	23	theme	dairy	153:157	arg1	cows					159:162	midlactation dairy cows	140:162	midlactation dairy cows	140:162	Effects of trace mineral amount and source on aspects of oxidative metabolism and responses to intramammary lipopolysaccharide challenge in midlactation dairy cows.
30322418	6	24	theme	Treatment	1406:1414	arg1	effects					1416:1422	Treatment effects	1406:1422	Treatment effects	1406:1422	Treatment effects were level, source and their interaction.
30322418	7	25	theme	acid	1588:1591	arg1	TBARS					1614:1618	TBARS	1614:1618	TBARS	1614:1618	Activities of super oxide dismutase and glutathione peroxidase in erythrocyte lysate and concentrations of thiobarbituric acid reactive substances (TBARS) and total antioxidant capacity (TAC) in plasma were measured as indices of oxidative metabolism.
30322418	7	25	theme	acid	1588:1591	arg1	substances					1602:1611	thiobarbituric acid reactive substances	1573:1611	thiobarbituric acid reactive substances (TBARS)	1573:1619	Activities of super oxide dismutase and glutathione peroxidase in erythrocyte lysate and concentrations of thiobarbituric acid reactive substances (TBARS) and total antioxidant capacity (TAC) in plasma were measured as indices of oxidative metabolism.
30322418	13	26	theme	intramammary	2833:2844	arg1	challenge					2850:2858	intramammary LPS challenge	2833:2858	intramammary LPS challenge in midlactation cows	2833:2879	Overall, results suggest that the varying level and source of dietary trace minerals do not have significant short-term effects on oxidative metabolism indices and clinical responses to intramammary LPS challenge in midlactation cows.
30322418	4	27	theme	NRC	1040:1042	arg1	levels					1044:1049	NRC levels	1040:1049	NRC levels using organic trace mineral supplements (metals chelated to 2-hydroxy-4-(methythio)-butanoic acid)	1040:1148	After a 4-week preliminary period, cows were assigned to one of four topdress treatments in a randomized complete block design with a 2×2 factorial arrangement of treatments: (1) NRC inorganic (NRC levels using inorganic (sulfate-based) trace mineral supplements only); (2) NRC organic (NRC levels using organic trace mineral supplements (metals chelated to 2-hydroxy-4-(methythio)-butanoic acid); (3) commercial inorganic (approximately 2×NRC levels using inorganic trace mineral supplements only; and (4) commercial organic (commercial levels using organic trace mineral supplements only).
30322418	2	28	theme	trace	478:482	arg1	amount					492:497	trace mineral amount	478:497	trace mineral amount	478:497	Multiparous Holstein cows (n=48) beginning at 60 to 140 days in milk were utilized to determine the effects of trace mineral amount and source on aspects of oxidative metabolism and responses to intramammary lipopolysaccharide (LPS) challenge.
30322418	11	29	theme	LPS	2339:2341	arg1	challenge					2343:2351	the LPS challenge	2335:2351	the LPS challenge	2335:2351	After the LPS challenge, the extent and pattern of response of plasma cortisol concentrations and clinical indices (rectal temperature and heart rate) were not affected by trace mineral level and source.
30322418	9	30	theme	week	2064:2067	arg1	end					2057:2059	the end	2053:2059	the end of week 2 of treatment period	2053:2089	Plasma immunoglobulin G level was higher in cows supplemented with organic trace minerals over the entire treatment period; responses assessed as differences of before and after Escherichia coli J5 bacterin vaccination at the end of week 2 of treatment period were not significant.
30322418	4	31	dep	one	810:812	arg1	inorganic					936:944	inorganic	936:944	inorganic	936:944	After a 4-week preliminary period, cows were assigned to one of four topdress treatments in a randomized complete block design with a 2×2 factorial arrangement of treatments: (1) NRC inorganic (NRC levels using inorganic (sulfate-based) trace mineral supplements only); (2) NRC organic (NRC levels using organic trace mineral supplements (metals chelated to 2-hydroxy-4-(methythio)-butanoic acid); (3) commercial inorganic (approximately 2×NRC levels using inorganic trace mineral supplements only; and (4) commercial organic (commercial levels using organic trace mineral supplements only).
30322418	4	31	dep	one	810:812	arg1	levels					1197:1202	approximately 2×NRC levels	1177:1202	one of four topdress treatments in a randomized complete block design with a 2×2 factorial arrangement of treatments: (1) NRC inorganic (NRC levels using inorganic (sulfate-based) trace mineral supplements only); (2) NRC organic (NRC levels using organic trace mineral supplements (metals chelated to 2-hydroxy-4-(methythio)-butanoic acid); (3) commercial inorganic (approximately 2×NRC levels using inorganic trace mineral supplements only;	810:1250	After a 4-week preliminary period, cows were assigned to one of four topdress treatments in a randomized complete block design with a 2×2 factorial arrangement of treatments: (1) NRC inorganic (NRC levels using inorganic (sulfate-based) trace mineral supplements only); (2) NRC organic (NRC levels using organic trace mineral supplements (metals chelated to 2-hydroxy-4-(methythio)-butanoic acid); (3) commercial inorganic (approximately 2×NRC levels using inorganic trace mineral supplements only; and (4) commercial organic (commercial levels using organic trace mineral supplements only).
30322418	4	32	theme	2×2	887:889	arg1	arrangement					901:911	a 2×2 factorial arrangement	885:911	a 2×2 factorial arrangement of treatments	885:925	After a 4-week preliminary period, cows were assigned to one of four topdress treatments in a randomized complete block design with a 2×2 factorial arrangement of treatments: (1) NRC inorganic (NRC levels using inorganic (sulfate-based) trace mineral supplements only); (2) NRC organic (NRC levels using organic trace mineral supplements (metals chelated to 2-hydroxy-4-(methythio)-butanoic acid); (3) commercial inorganic (approximately 2×NRC levels using inorganic trace mineral supplements only; and (4) commercial organic (commercial levels using organic trace mineral supplements only).
30322418	9	33	theme	immunoglobulin	1838:1851	arg1	G					1853:1853	Plasma immunoglobulin G	1831:1853	Plasma immunoglobulin G level	1831:1859	Plasma immunoglobulin G level was higher in cows supplemented with organic trace minerals over the entire treatment period; responses assessed as differences of before and after Escherichia coli J5 bacterin vaccination at the end of week 2 of treatment period were not significant.
30322418	0	34	theme	oxidative	57:65	arg1	metabolism					67:76	oxidative metabolism	57:76	oxidative metabolism	57:76	Effects of trace mineral amount and source on aspects of oxidative metabolism and responses to intramammary lipopolysaccharide challenge in midlactation dairy cows.
30322418	1	35	theme	Trace	165:169	arg1	minerals					171:178	Trace minerals	165:178	Trace minerals	165:178	Trace minerals have important roles in immune function and oxidative metabolism; however, little is known about the relationships between supplementation level and source with outcomes in dairy cattle.
30322418	7	36	theme	erythrocyte	1532:1542	arg1	lysate					1544:1549	erythrocyte lysate	1532:1549	erythrocyte lysate	1532:1549	Activities of super oxide dismutase and glutathione peroxidase in erythrocyte lysate and concentrations of thiobarbituric acid reactive substances (TBARS) and total antioxidant capacity (TAC) in plasma were measured as indices of oxidative metabolism.
30322418	9	37	theme	organic	1898:1904	arg1	minerals					1912:1919	organic trace minerals	1898:1919	organic trace minerals over the entire treatment period	1898:1952	Plasma immunoglobulin G level was higher in cows supplemented with organic trace minerals over the entire treatment period; responses assessed as differences of before and after Escherichia coli J5 bacterin vaccination at the end of week 2 of treatment period were not significant.
30322418	2	38	theme	source	503:508	arg1	effects					467:473	the effects	463:473	the effects of trace mineral amount and source on aspects of oxidative metabolism and responses to intramammary lipopolysaccharide (LPS) challenge	463:608	Multiparous Holstein cows (n=48) beginning at 60 to 140 days in milk were utilized to determine the effects of trace mineral amount and source on aspects of oxidative metabolism and responses to intramammary lipopolysaccharide (LPS) challenge.
30322418	0	39	from	Effects	0:6	arg1	responses					82:90	responses	82:90	responses to intramammary lipopolysaccharide challenge in midlactation dairy cows	82:162	Effects of trace mineral amount and source on aspects of oxidative metabolism and responses to intramammary lipopolysaccharide challenge in midlactation dairy cows.
30322418	0	39	from	Effects	0:6	arg1	aspects					46:52	aspects	46:52	aspects of oxidative metabolism	46:76	Effects of trace mineral amount and source on aspects of oxidative metabolism and responses to intramammary lipopolysaccharide challenge in midlactation dairy cows.
30322418	9	40	theme	period	2084:2089	arg1	week					2064:2067	week 2	2064:2069	week 2 of treatment period	2064:2089	Plasma immunoglobulin G level was higher in cows supplemented with organic trace minerals over the entire treatment period; responses assessed as differences of before and after Escherichia coli J5 bacterin vaccination at the end of week 2 of treatment period were not significant.
30322418	4	41	dep	inorganic	936:944	arg1	3					1152:1152	3	1152:1152	3	1152:1152	After a 4-week preliminary period, cows were assigned to one of four topdress treatments in a randomized complete block design with a 2×2 factorial arrangement of treatments: (1) NRC inorganic (NRC levels using inorganic (sulfate-based) trace mineral supplements only); (2) NRC organic (NRC levels using organic trace mineral supplements (metals chelated to 2-hydroxy-4-(methythio)-butanoic acid); (3) commercial inorganic (approximately 2×NRC levels using inorganic trace mineral supplements only; and (4) commercial organic (commercial levels using organic trace mineral supplements only).
30322418	7	42	theme	peroxidase	1518:1527	arg1	indices					1685:1691	indices	1685:1691	indices of oxidative metabolism	1685:1715	Activities of super oxide dismutase and glutathione peroxidase in erythrocyte lysate and concentrations of thiobarbituric acid reactive substances (TBARS) and total antioxidant capacity (TAC) in plasma were measured as indices of oxidative metabolism.
30322418	7	42	theme	peroxidase	1518:1527	arg1	concentrations					1555:1568	concentrations	1555:1568	concentrations of thiobarbituric acid reactive substances (TBARS) and total antioxidant capacity (TAC) in plasma	1555:1666	Activities of super oxide dismutase and glutathione peroxidase in erythrocyte lysate and concentrations of thiobarbituric acid reactive substances (TBARS) and total antioxidant capacity (TAC) in plasma were measured as indices of oxidative metabolism.
30322418	7	42	theme	peroxidase	1518:1527	arg1	Activities					1466:1475	Activities	1466:1475	Activities of super oxide dismutase and glutathione peroxidase in erythrocyte lysate	1466:1549	Activities of super oxide dismutase and glutathione peroxidase in erythrocyte lysate and concentrations of thiobarbituric acid reactive substances (TBARS) and total antioxidant capacity (TAC) in plasma were measured as indices of oxidative metabolism.
30322418	11	43	theme	heart	2468:2472	arg1	rate					2474:2477	heart rate	2468:2477	heart rate	2468:2477	After the LPS challenge, the extent and pattern of response of plasma cortisol concentrations and clinical indices (rectal temperature and heart rate) were not affected by trace mineral level and source.
30322418	11	43	theme	heart	2468:2472	arg1	indices					2436:2442	clinical indices	2427:2442	clinical indices (rectal temperature and heart rate)	2427:2478	After the LPS challenge, the extent and pattern of response of plasma cortisol concentrations and clinical indices (rectal temperature and heart rate) were not affected by trace mineral level and source.
30322418	2	44	theme	amount	492:497	arg1	effects					467:473	the effects	463:473	the effects of trace mineral amount and source on aspects of oxidative metabolism and responses to intramammary lipopolysaccharide (LPS) challenge	463:608	Multiparous Holstein cows (n=48) beginning at 60 to 140 days in milk were utilized to determine the effects of trace mineral amount and source on aspects of oxidative metabolism and responses to intramammary lipopolysaccharide (LPS) challenge.
30322418	4	45	theme	mineral	1318:1324	arg1	supplements					1326:1336	organic trace mineral supplements	1304:1336	organic trace mineral supplements only	1304:1341	After a 4-week preliminary period, cows were assigned to one of four topdress treatments in a randomized complete block design with a 2×2 factorial arrangement of treatments: (1) NRC inorganic (NRC levels using inorganic (sulfate-based) trace mineral supplements only); (2) NRC organic (NRC levels using organic trace mineral supplements (metals chelated to 2-hydroxy-4-(methythio)-butanoic acid); (3) commercial inorganic (approximately 2×NRC levels using inorganic trace mineral supplements only; and (4) commercial organic (commercial levels using organic trace mineral supplements only).
30322418	2	46	dep	140	419:421	arg1	to					416:417	to	416:417	to	416:417	Multiparous Holstein cows (n=48) beginning at 60 to 140 days in milk were utilized to determine the effects of trace mineral amount and source on aspects of oxidative metabolism and responses to intramammary lipopolysaccharide (LPS) challenge.
30322418	12	47	theme	milk	2588:2591	arg1	yield					2593:2597	milk yield	2588:2597	milk yield	2588:2597	Productive performance including dry matter intake and milk yield and composition were not affected by treatment.
30322418	10	48	theme	commercial	2204:2213	arg1	levels					2215:2220	commercial levels	2204:2220	commercial levels of Zn, Cu and Mn	2204:2237	Cows were administered an intramammary LPS challenge during week 5; during week 6 cows fed commercial levels of Zn, Cu and Mn tended to have higher plasma TAC and cows fed organic sources had decreased plasma TBARS.
30322418	7	49	from	Activities	1466:1475	arg1	lysate					1544:1549	erythrocyte lysate	1532:1549	erythrocyte lysate	1532:1549	Activities of super oxide dismutase and glutathione peroxidase in erythrocyte lysate and concentrations of thiobarbituric acid reactive substances (TBARS) and total antioxidant capacity (TAC) in plasma were measured as indices of oxidative metabolism.
30322418	7	49	from	Activities	1466:1475	arg1	plasma					1661:1666	plasma	1661:1666	plasma	1661:1666	Activities of super oxide dismutase and glutathione peroxidase in erythrocyte lysate and concentrations of thiobarbituric acid reactive substances (TBARS) and total antioxidant capacity (TAC) in plasma were measured as indices of oxidative metabolism.
30322418	7	50	theme	oxide	1486:1490	arg1	dismutase					1492:1500	super oxide dismutase	1480:1500	super oxide dismutase	1480:1500	Activities of super oxide dismutase and glutathione peroxidase in erythrocyte lysate and concentrations of thiobarbituric acid reactive substances (TBARS) and total antioxidant capacity (TAC) in plasma were measured as indices of oxidative metabolism.
30322418	9	51	theme	trace	1906:1910	arg1	minerals					1912:1919	organic trace minerals	1898:1919	organic trace minerals over the entire treatment period	1898:1952	Plasma immunoglobulin G level was higher in cows supplemented with organic trace minerals over the entire treatment period; responses assessed as differences of before and after Escherichia coli J5 bacterin vaccination at the end of week 2 of treatment period were not significant.
30322418	11	52	theme	clinical	2427:2434	arg1	rate					2474:2477	heart rate	2468:2477	heart rate	2468:2477	After the LPS challenge, the extent and pattern of response of plasma cortisol concentrations and clinical indices (rectal temperature and heart rate) were not affected by trace mineral level and source.
30322418	11	52	theme	clinical	2427:2434	arg1	indices					2436:2442	clinical indices	2427:2442	clinical indices (rectal temperature and heart rate)	2427:2478	After the LPS challenge, the extent and pattern of response of plasma cortisol concentrations and clinical indices (rectal temperature and heart rate) were not affected by trace mineral level and source.
30322418	11	52	theme	clinical	2427:2434	arg1	temperature					2452:2462	rectal temperature	2445:2462	rectal temperature	2445:2462	After the LPS challenge, the extent and pattern of response of plasma cortisol concentrations and clinical indices (rectal temperature and heart rate) were not affected by trace mineral level and source.
30322418	4	53	theme	trace	1065:1069	arg1	supplements					1079:1089	organic trace mineral supplements	1057:1089	organic trace mineral supplements (metals chelated to 2-hydroxy-4-(methythio)-butanoic acid)	1057:1148	After a 4-week preliminary period, cows were assigned to one of four topdress treatments in a randomized complete block design with a 2×2 factorial arrangement of treatments: (1) NRC inorganic (NRC levels using inorganic (sulfate-based) trace mineral supplements only); (2) NRC organic (NRC levels using organic trace mineral supplements (metals chelated to 2-hydroxy-4-(methythio)-butanoic acid); (3) commercial inorganic (approximately 2×NRC levels using inorganic trace mineral supplements only; and (4) commercial organic (commercial levels using organic trace mineral supplements only).
30322418	0	54	theme	trace	11:15	arg1	amount					25:30	trace mineral amount	11:30	trace mineral amount	11:30	Effects of trace mineral amount and source on aspects of oxidative metabolism and responses to intramammary lipopolysaccharide challenge in midlactation dairy cows.
30322418	13	55	theme	minerals	2723:2730	arg1	source					2699:2704	source	2699:2704	source	2699:2704	Overall, results suggest that the varying level and source of dietary trace minerals do not have significant short-term effects on oxidative metabolism indices and clinical responses to intramammary LPS challenge in midlactation cows.
30322418	13	55	theme	minerals	2723:2730	arg1	level					2689:2693	varying level	2681:2693	varying level	2681:2693	Overall, results suggest that the varying level and source of dietary trace minerals do not have significant short-term effects on oxidative metabolism indices and clinical responses to intramammary LPS challenge in midlactation cows.
30322418	10	56	theme	plasma	2261:2266	arg1	TAC					2268:2270	higher plasma TAC	2254:2270	higher plasma TAC	2254:2270	Cows were administered an intramammary LPS challenge during week 5; during week 6 cows fed commercial levels of Zn, Cu and Mn tended to have higher plasma TAC and cows fed organic sources had decreased plasma TBARS.
30322418	11	57	theme	concentrations	2408:2421	arg1	response					2380:2387	response	2380:2387	response of plasma cortisol concentrations and clinical indices (rectal temperature and heart rate)	2380:2478	After the LPS challenge, the extent and pattern of response of plasma cortisol concentrations and clinical indices (rectal temperature and heart rate) were not affected by trace mineral level and source.
30322418	5	58	theme	respective	1363:1372	arg1	treatments					1382:1391	the respective mineral treatments	1359:1391	the respective mineral treatments	1359:1391	Cows were fed the respective mineral treatments for 6 weeks.
30322418	3	59	theme	basal	627:631	arg1	diet					633:636	a basal diet	625:636	a basal diet meeting National Research Council (NRC) requirements	625:689	Cows were fed a basal diet meeting National Research Council (NRC) requirements except for no added zinc (Zn), copper (Cu) or manganese (Mn).
30322418	4	60	theme	organic	1304:1310	arg1	supplements					1326:1336	organic trace mineral supplements	1304:1336	organic trace mineral supplements only	1304:1341	After a 4-week preliminary period, cows were assigned to one of four topdress treatments in a randomized complete block design with a 2×2 factorial arrangement of treatments: (1) NRC inorganic (NRC levels using inorganic (sulfate-based) trace mineral supplements only); (2) NRC organic (NRC levels using organic trace mineral supplements (metals chelated to 2-hydroxy-4-(methythio)-butanoic acid); (3) commercial inorganic (approximately 2×NRC levels using inorganic trace mineral supplements only; and (4) commercial organic (commercial levels using organic trace mineral supplements only).
30322418	0	61	theme	amount	25:30	arg1	Effects					0:6	Effects	0:6	Effects of trace mineral amount and source on aspects of oxidative metabolism and responses to intramammary lipopolysaccharide challenge in midlactation dairy cows.	0:163	Effects of trace mineral amount and source on aspects of oxidative metabolism and responses to intramammary lipopolysaccharide challenge in midlactation dairy cows.
30322418	10	62	theme	higher	2254:2259	arg1	TAC					2268:2270	higher plasma TAC	2254:2270	higher plasma TAC	2254:2270	Cows were administered an intramammary LPS challenge during week 5; during week 6 cows fed commercial levels of Zn, Cu and Mn tended to have higher plasma TAC and cows fed organic sources had decreased plasma TBARS.
30322418	12	63	theme	Productive	2533:2542	arg1	performance					2544:2554	Productive performance	2533:2554	Productive performance including dry matter intake and milk yield and composition	2533:2613	Productive performance including dry matter intake and milk yield and composition were not affected by treatment.
30322418	4	64	theme	treatments	916:925	arg1	arrangement					901:911	a 2×2 factorial arrangement	885:911	a 2×2 factorial arrangement of treatments	885:925	After a 4-week preliminary period, cows were assigned to one of four topdress treatments in a randomized complete block design with a 2×2 factorial arrangement of treatments: (1) NRC inorganic (NRC levels using inorganic (sulfate-based) trace mineral supplements only); (2) NRC organic (NRC levels using organic trace mineral supplements (metals chelated to 2-hydroxy-4-(methythio)-butanoic acid); (3) commercial inorganic (approximately 2×NRC levels using inorganic trace mineral supplements only; and (4) commercial organic (commercial levels using organic trace mineral supplements only).
30322418	13	65	dep	metabolism	2788:2797	arg1	indices					2799:2805	indices	2799:2805	indices	2799:2805	Overall, results suggest that the varying level and source of dietary trace minerals do not have significant short-term effects on oxidative metabolism indices and clinical responses to intramammary LPS challenge in midlactation cows.
30322418	0	66	theme	source	36:41	arg1	Effects					0:6	Effects	0:6	Effects of trace mineral amount and source on aspects of oxidative metabolism and responses to intramammary lipopolysaccharide challenge in midlactation dairy cows.	0:163	Effects of trace mineral amount and source on aspects of oxidative metabolism and responses to intramammary lipopolysaccharide challenge in midlactation dairy cows.
30322418	7	67	theme	metabolism	1706:1715	arg1	indices					1685:1691	indices	1685:1691	indices of oxidative metabolism	1685:1715	Activities of super oxide dismutase and glutathione peroxidase in erythrocyte lysate and concentrations of thiobarbituric acid reactive substances (TBARS) and total antioxidant capacity (TAC) in plasma were measured as indices of oxidative metabolism.
30322418	7	67	theme	metabolism	1706:1715	arg1	concentrations					1555:1568	concentrations	1555:1568	concentrations of thiobarbituric acid reactive substances (TBARS) and total antioxidant capacity (TAC) in plasma	1555:1666	Activities of super oxide dismutase and glutathione peroxidase in erythrocyte lysate and concentrations of thiobarbituric acid reactive substances (TBARS) and total antioxidant capacity (TAC) in plasma were measured as indices of oxidative metabolism.
30322418	7	67	theme	metabolism	1706:1715	arg1	Activities					1466:1475	Activities	1466:1475	Activities of super oxide dismutase and glutathione peroxidase in erythrocyte lysate	1466:1549	Activities of super oxide dismutase and glutathione peroxidase in erythrocyte lysate and concentrations of thiobarbituric acid reactive substances (TBARS) and total antioxidant capacity (TAC) in plasma were measured as indices of oxidative metabolism.
30322418	3	68	theme	Research	655:662	arg1	NRC					673:675	NRC	673:675	NRC	673:675	Cows were fed a basal diet meeting National Research Council (NRC) requirements except for no added zinc (Zn), copper (Cu) or manganese (Mn).
30322418	3	68	theme	Research	655:662	arg1	Council					664:670	National Research Council	646:670	National Research Council (NRC) requirements	646:689	Cows were fed a basal diet meeting National Research Council (NRC) requirements except for no added zinc (Zn), copper (Cu) or manganese (Mn).
30322418	1	69	theme	oxidative	224:232	arg1	metabolism					234:243	oxidative metabolism	224:243	oxidative metabolism	224:243	Trace minerals have important roles in immune function and oxidative metabolism; however, little is known about the relationships between supplementation level and source with outcomes in dairy cattle.
30322418	10	70	dep	fed	2200:2202	arg1	tended					2239:2244	tended	2239:2244	fed commercial levels of Zn, Cu and Mn tended to have higher plasma TAC	2200:2270	Cows were administered an intramammary LPS challenge during week 5; during week 6 cows fed commercial levels of Zn, Cu and Mn tended to have higher plasma TAC and cows fed organic sources had decreased plasma TBARS.
30322418	4	71	dep	commercial	1260:1269	arg1	organic					1271:1277	organic	1271:1277	organic	1271:1277	After a 4-week preliminary period, cows were assigned to one of four topdress treatments in a randomized complete block design with a 2×2 factorial arrangement of treatments: (1) NRC inorganic (NRC levels using inorganic (sulfate-based) trace mineral supplements only); (2) NRC organic (NRC levels using organic trace mineral supplements (metals chelated to 2-hydroxy-4-(methythio)-butanoic acid); (3) commercial inorganic (approximately 2×NRC levels using inorganic trace mineral supplements only; and (4) commercial organic (commercial levels using organic trace mineral supplements only).
30322418	7	72	theme	capacity	1643:1650	arg1	indices					1685:1691	indices	1685:1691	indices of oxidative metabolism	1685:1715	Activities of super oxide dismutase and glutathione peroxidase in erythrocyte lysate and concentrations of thiobarbituric acid reactive substances (TBARS) and total antioxidant capacity (TAC) in plasma were measured as indices of oxidative metabolism.
30322418	7	72	theme	capacity	1643:1650	arg1	concentrations					1555:1568	concentrations	1555:1568	concentrations of thiobarbituric acid reactive substances (TBARS) and total antioxidant capacity (TAC) in plasma	1555:1666	Activities of super oxide dismutase and glutathione peroxidase in erythrocyte lysate and concentrations of thiobarbituric acid reactive substances (TBARS) and total antioxidant capacity (TAC) in plasma were measured as indices of oxidative metabolism.
30322418	7	72	theme	capacity	1643:1650	arg1	Activities					1466:1475	Activities	1466:1475	Activities of super oxide dismutase and glutathione peroxidase in erythrocyte lysate	1466:1549	Activities of super oxide dismutase and glutathione peroxidase in erythrocyte lysate and concentrations of thiobarbituric acid reactive substances (TBARS) and total antioxidant capacity (TAC) in plasma were measured as indices of oxidative metabolism.
30322418	2	73	theme	Holstein	379:386	arg1	n=48					394:397	n=48	394:397	n=48	394:397	Multiparous Holstein cows (n=48) beginning at 60 to 140 days in milk were utilized to determine the effects of trace mineral amount and source on aspects of oxidative metabolism and responses to intramammary lipopolysaccharide (LPS) challenge.
30322418	2	73	theme	Holstein	379:386	arg1	cows					388:391	Multiparous Holstein cows	367:391	Multiparous Holstein cows (n=48) beginning at 60 to 140 days in milk	367:434	Multiparous Holstein cows (n=48) beginning at 60 to 140 days in milk were utilized to determine the effects of trace mineral amount and source on aspects of oxidative metabolism and responses to intramammary lipopolysaccharide (LPS) challenge.
30322418	2	74	used	utilized	441:448	arg2	cows					388:391	Multiparous Holstein cows	367:391	Multiparous Holstein cows (n=48) beginning at 60 to 140 days in milk	367:434	Multiparous Holstein cows (n=48) beginning at 60 to 140 days in milk were utilized to determine the effects of trace mineral amount and source on aspects of oxidative metabolism and responses to intramammary lipopolysaccharide (LPS) challenge.
30322418	2	74	used	utilized	441:448	arg2	n=48					394:397	n=48	394:397	n=48	394:397	Multiparous Holstein cows (n=48) beginning at 60 to 140 days in milk were utilized to determine the effects of trace mineral amount and source on aspects of oxidative metabolism and responses to intramammary lipopolysaccharide (LPS) challenge.
30322418	7	75	theme	antioxidant	1631:1641	arg1	TAC					1653:1655	TAC	1653:1655	TAC	1653:1655	Activities of super oxide dismutase and glutathione peroxidase in erythrocyte lysate and concentrations of thiobarbituric acid reactive substances (TBARS) and total antioxidant capacity (TAC) in plasma were measured as indices of oxidative metabolism.
30322418	7	75	theme	antioxidant	1631:1641	arg1	capacity					1643:1650	total antioxidant capacity	1625:1650	total antioxidant capacity (TAC)	1625:1656	Activities of super oxide dismutase and glutathione peroxidase in erythrocyte lysate and concentrations of thiobarbituric acid reactive substances (TBARS) and total antioxidant capacity (TAC) in plasma were measured as indices of oxidative metabolism.
30322418	4	76	theme	NRC	947:949	arg1	levels					951:956	NRC levels	947:956	NRC levels	947:956	After a 4-week preliminary period, cows were assigned to one of four topdress treatments in a randomized complete block design with a 2×2 factorial arrangement of treatments: (1) NRC inorganic (NRC levels using inorganic (sulfate-based) trace mineral supplements only); (2) NRC organic (NRC levels using organic trace mineral supplements (metals chelated to 2-hydroxy-4-(methythio)-butanoic acid); (3) commercial inorganic (approximately 2×NRC levels using inorganic trace mineral supplements only; and (4) commercial organic (commercial levels using organic trace mineral supplements only).
30322418	11	77	theme	plasma	2392:2397	arg1	concentrations					2408:2421	plasma cortisol concentrations	2392:2421	plasma cortisol concentrations	2392:2421	After the LPS challenge, the extent and pattern of response of plasma cortisol concentrations and clinical indices (rectal temperature and heart rate) were not affected by trace mineral level and source.
30322418	4	78	dep	supplements	1079:1089	arg1	metals					1092:1097	metals	1092:1097	metals chelated to 2-hydroxy-4-(methythio)-butanoic acid	1092:1147	After a 4-week preliminary period, cows were assigned to one of four topdress treatments in a randomized complete block design with a 2×2 factorial arrangement of treatments: (1) NRC inorganic (NRC levels using inorganic (sulfate-based) trace mineral supplements only); (2) NRC organic (NRC levels using organic trace mineral supplements (metals chelated to 2-hydroxy-4-(methythio)-butanoic acid); (3) commercial inorganic (approximately 2×NRC levels using inorganic trace mineral supplements only; and (4) commercial organic (commercial levels using organic trace mineral supplements only).
30322418	11	79	dep	indices	2436:2442	arg1	rate					2474:2477	heart rate	2468:2477	heart rate	2468:2477	After the LPS challenge, the extent and pattern of response of plasma cortisol concentrations and clinical indices (rectal temperature and heart rate) were not affected by trace mineral level and source.
30322418	11	79	dep	indices	2436:2442	arg1	indices					2436:2442	clinical indices	2427:2442	clinical indices (rectal temperature and heart rate)	2427:2478	After the LPS challenge, the extent and pattern of response of plasma cortisol concentrations and clinical indices (rectal temperature and heart rate) were not affected by trace mineral level and source.
30322418	11	79	dep	indices	2436:2442	arg1	temperature					2452:2462	rectal temperature	2445:2462	rectal temperature	2445:2462	After the LPS challenge, the extent and pattern of response of plasma cortisol concentrations and clinical indices (rectal temperature and heart rate) were not affected by trace mineral level and source.
30322418	2	80	theme	intramammary	562:573	arg1	challenge					600:608	intramammary lipopolysaccharide (LPS) challenge	562:608	intramammary lipopolysaccharide (LPS) challenge	562:608	Multiparous Holstein cows (n=48) beginning at 60 to 140 days in milk were utilized to determine the effects of trace mineral amount and source on aspects of oxidative metabolism and responses to intramammary lipopolysaccharide (LPS) challenge.
30322418	1	81	with	supplementation	303:317	arg1	outcomes					341:348	outcomes	341:348	outcomes in dairy cattle	341:364	Trace minerals have important roles in immune function and oxidative metabolism; however, little is known about the relationships between supplementation level and source with outcomes in dairy cattle.
30322418	13	82	contain	have	2739:2742	arg1	source					2699:2704	source	2699:2704	source	2699:2704	Overall, results suggest that the varying level and source of dietary trace minerals do not have significant short-term effects on oxidative metabolism indices and clinical responses to intramammary LPS challenge in midlactation cows.
30322418	13	82	contain	have	2739:2742	arg1	level					2689:2693	varying level	2681:2693	varying level	2681:2693	Overall, results suggest that the varying level and source of dietary trace minerals do not have significant short-term effects on oxidative metabolism indices and clinical responses to intramammary LPS challenge in midlactation cows.
30322418	13	82	contain	have	2739:2742	arg2	effects					2767:2773	significant short-term effects	2744:2773	significant short-term effects	2744:2773	Overall, results suggest that the varying level and source of dietary trace minerals do not have significant short-term effects on oxidative metabolism indices and clinical responses to intramammary LPS challenge in midlactation cows.
30322418	11	83	theme	response	2380:2387	arg1	pattern					2369:2375	pattern	2369:2375	pattern	2369:2375	After the LPS challenge, the extent and pattern of response of plasma cortisol concentrations and clinical indices (rectal temperature and heart rate) were not affected by trace mineral level and source.
30322418	11	83	theme	response	2380:2387	arg1	extent					2358:2363	extent	2358:2363	extent	2358:2363	After the LPS challenge, the extent and pattern of response of plasma cortisol concentrations and clinical indices (rectal temperature and heart rate) were not affected by trace mineral level and source.
30322418	1	84	from	outcomes	341:348	arg1	cattle					359:364	dairy cattle	353:364	dairy cattle	353:364	Trace minerals have important roles in immune function and oxidative metabolism; however, little is known about the relationships between supplementation level and source with outcomes in dairy cattle.
30322418	7	85	from	concentrations	1555:1568	arg1	lysate					1544:1549	erythrocyte lysate	1532:1549	erythrocyte lysate	1532:1549	Activities of super oxide dismutase and glutathione peroxidase in erythrocyte lysate and concentrations of thiobarbituric acid reactive substances (TBARS) and total antioxidant capacity (TAC) in plasma were measured as indices of oxidative metabolism.
30322418	7	85	from	concentrations	1555:1568	arg1	plasma					1661:1666	plasma	1661:1666	plasma	1661:1666	Activities of super oxide dismutase and glutathione peroxidase in erythrocyte lysate and concentrations of thiobarbituric acid reactive substances (TBARS) and total antioxidant capacity (TAC) in plasma were measured as indices of oxidative metabolism.
30322418	11	86	theme	indices	2436:2442	arg1	response					2380:2387	response	2380:2387	response of plasma cortisol concentrations and clinical indices (rectal temperature and heart rate)	2380:2478	After the LPS challenge, the extent and pattern of response of plasma cortisol concentrations and clinical indices (rectal temperature and heart rate) were not affected by trace mineral level and source.
30322418	8	87	theme	entire	1803:1808	arg1	period					1823:1828	the entire experimental period	1799:1828	the entire experimental period	1799:1828	Effects of treatment on those indices were not significant when evaluated across the entire experimental period.
30322418	8	88	theme	treatment	1729:1737	arg1	Effects					1718:1724	Effects	1718:1724	Effects of treatment on those indices	1718:1754	Effects of treatment on those indices were not significant when evaluated across the entire experimental period.
30322418	13	89	dep	level	2689:2693	arg1	the					2677:2679	the	2677:2679	the	2677:2679	Overall, results suggest that the varying level and source of dietary trace minerals do not have significant short-term effects on oxidative metabolism indices and clinical responses to intramammary LPS challenge in midlactation cows.
30322418	1	90	theme	important	185:193	arg1	roles					195:199	important roles	185:199	important roles	185:199	Trace minerals have important roles in immune function and oxidative metabolism; however, little is known about the relationships between supplementation level and source with outcomes in dairy cattle.
30322418	13	91	theme	LPS	2846:2848	arg1	challenge					2850:2858	intramammary LPS challenge	2833:2858	intramammary LPS challenge in midlactation cows	2833:2879	Overall, results suggest that the varying level and source of dietary trace minerals do not have significant short-term effects on oxidative metabolism indices and clinical responses to intramammary LPS challenge in midlactation cows.
30322418	4	92	theme	4-week	761:766	arg1	period					780:785	a 4-week preliminary period	759:785	a 4-week preliminary period	759:785	After a 4-week preliminary period, cows were assigned to one of four topdress treatments in a randomized complete block design with a 2×2 factorial arrangement of treatments: (1) NRC inorganic (NRC levels using inorganic (sulfate-based) trace mineral supplements only); (2) NRC organic (NRC levels using organic trace mineral supplements (metals chelated to 2-hydroxy-4-(methythio)-butanoic acid); (3) commercial inorganic (approximately 2×NRC levels using inorganic trace mineral supplements only; and (4) commercial organic (commercial levels using organic trace mineral supplements only).
30322418	7	93	theme	substances	1602:1611	arg1	indices					1685:1691	indices	1685:1691	indices of oxidative metabolism	1685:1715	Activities of super oxide dismutase and glutathione peroxidase in erythrocyte lysate and concentrations of thiobarbituric acid reactive substances (TBARS) and total antioxidant capacity (TAC) in plasma were measured as indices of oxidative metabolism.
30322418	7	93	theme	substances	1602:1611	arg1	concentrations					1555:1568	concentrations	1555:1568	concentrations of thiobarbituric acid reactive substances (TBARS) and total antioxidant capacity (TAC) in plasma	1555:1666	Activities of super oxide dismutase and glutathione peroxidase in erythrocyte lysate and concentrations of thiobarbituric acid reactive substances (TBARS) and total antioxidant capacity (TAC) in plasma were measured as indices of oxidative metabolism.
30322418	7	93	theme	substances	1602:1611	arg1	Activities					1466:1475	Activities	1466:1475	Activities of super oxide dismutase and glutathione peroxidase in erythrocyte lysate	1466:1549	Activities of super oxide dismutase and glutathione peroxidase in erythrocyte lysate and concentrations of thiobarbituric acid reactive substances (TBARS) and total antioxidant capacity (TAC) in plasma were measured as indices of oxidative metabolism.
30322418	11	94	theme	mineral	2507:2513	arg1	level					2515:2519	trace mineral level	2501:2519	trace mineral level	2501:2519	After the LPS challenge, the extent and pattern of response of plasma cortisol concentrations and clinical indices (rectal temperature and heart rate) were not affected by trace mineral level and source.
30322418	4	95	theme	trace	1220:1224	arg1	supplements					1234:1244	inorganic trace mineral supplements	1210:1244	inorganic trace mineral supplements only	1210:1249	After a 4-week preliminary period, cows were assigned to one of four topdress treatments in a randomized complete block design with a 2×2 factorial arrangement of treatments: (1) NRC inorganic (NRC levels using inorganic (sulfate-based) trace mineral supplements only); (2) NRC organic (NRC levels using organic trace mineral supplements (metals chelated to 2-hydroxy-4-(methythio)-butanoic acid); (3) commercial inorganic (approximately 2×NRC levels using inorganic trace mineral supplements only; and (4) commercial organic (commercial levels using organic trace mineral supplements only).
30322418	13	96	theme	short-term	2756:2765	arg1	effects					2767:2773	significant short-term effects	2744:2773	significant short-term effects	2744:2773	Overall, results suggest that the varying level and source of dietary trace minerals do not have significant short-term effects on oxidative metabolism indices and clinical responses to intramammary LPS challenge in midlactation cows.
30322418	2	97	from	effects	467:473	arg1	responses					549:557	responses	549:557	responses to intramammary lipopolysaccharide (LPS) challenge	549:608	Multiparous Holstein cows (n=48) beginning at 60 to 140 days in milk were utilized to determine the effects of trace mineral amount and source on aspects of oxidative metabolism and responses to intramammary lipopolysaccharide (LPS) challenge.
30322418	2	97	from	effects	467:473	arg1	aspects					513:519	aspects	513:519	aspects of oxidative metabolism	513:543	Multiparous Holstein cows (n=48) beginning at 60 to 140 days in milk were utilized to determine the effects of trace mineral amount and source on aspects of oxidative metabolism and responses to intramammary lipopolysaccharide (LPS) challenge.
30322418	4	98	theme	mineral	996:1002	arg1	supplements					1004:1014	inorganic (sulfate-based) trace mineral supplements	964:1014	inorganic (sulfate-based) trace mineral supplements only	964:1019	After a 4-week preliminary period, cows were assigned to one of four topdress treatments in a randomized complete block design with a 2×2 factorial arrangement of treatments: (1) NRC inorganic (NRC levels using inorganic (sulfate-based) trace mineral supplements only); (2) NRC organic (NRC levels using organic trace mineral supplements (metals chelated to 2-hydroxy-4-(methythio)-butanoic acid); (3) commercial inorganic (approximately 2×NRC levels using inorganic trace mineral supplements only; and (4) commercial organic (commercial levels using organic trace mineral supplements only).
30322418	9	99	theme	J5	2026:2027	arg1	vaccination					2038:2048	Escherichia coli J5 bacterin vaccination	2009:2048	Escherichia coli J5 bacterin vaccination	2009:2048	Plasma immunoglobulin G level was higher in cows supplemented with organic trace minerals over the entire treatment period; responses assessed as differences of before and after Escherichia coli J5 bacterin vaccination at the end of week 2 of treatment period were not significant.
30322418	0	100	theme	midlactation	140:151	arg1	cows					159:162	midlactation dairy cows	140:162	midlactation dairy cows	140:162	Effects of trace mineral amount and source on aspects of oxidative metabolism and responses to intramammary lipopolysaccharide challenge in midlactation dairy cows.
30322418	4	101	from	treatments	831:840	arg1	design					873:878	a randomized complete block design	845:878	a randomized complete block design with a 2×2 factorial arrangement of treatments	845:925	After a 4-week preliminary period, cows were assigned to one of four topdress treatments in a randomized complete block design with a 2×2 factorial arrangement of treatments: (1) NRC inorganic (NRC levels using inorganic (sulfate-based) trace mineral supplements only); (2) NRC organic (NRC levels using organic trace mineral supplements (metals chelated to 2-hydroxy-4-(methythio)-butanoic acid); (3) commercial inorganic (approximately 2×NRC levels using inorganic trace mineral supplements only; and (4) commercial organic (commercial levels using organic trace mineral supplements only).
30322418	4	102	dep	organic	1031:1037	arg1	levels					1044:1049	NRC levels	1040:1049	NRC levels using organic trace mineral supplements (metals chelated to 2-hydroxy-4-(methythio)-butanoic acid)	1040:1148	After a 4-week preliminary period, cows were assigned to one of four topdress treatments in a randomized complete block design with a 2×2 factorial arrangement of treatments: (1) NRC inorganic (NRC levels using inorganic (sulfate-based) trace mineral supplements only); (2) NRC organic (NRC levels using organic trace mineral supplements (metals chelated to 2-hydroxy-4-(methythio)-butanoic acid); (3) commercial inorganic (approximately 2×NRC levels using inorganic trace mineral supplements only; and (4) commercial organic (commercial levels using organic trace mineral supplements only).
30322418	13	103	theme	clinical	2811:2818	arg1	responses					2820:2828	clinical responses	2811:2828	clinical responses to intramammary LPS challenge in midlactation cows	2811:2879	Overall, results suggest that the varying level and source of dietary trace minerals do not have significant short-term effects on oxidative metabolism indices and clinical responses to intramammary LPS challenge in midlactation cows.
30322418	4	104	theme	treatments	831:840	arg1	one					810:812	one	810:812	one	810:812	After a 4-week preliminary period, cows were assigned to one of four topdress treatments in a randomized complete block design with a 2×2 factorial arrangement of treatments: (1) NRC inorganic (NRC levels using inorganic (sulfate-based) trace mineral supplements only); (2) NRC organic (NRC levels using organic trace mineral supplements (metals chelated to 2-hydroxy-4-(methythio)-butanoic acid); (3) commercial inorganic (approximately 2×NRC levels using inorganic trace mineral supplements only; and (4) commercial organic (commercial levels using organic trace mineral supplements only).
30322418	4	104	theme	treatments	831:840	arg1	treatments					831:840	four topdress treatments	817:840	four topdress treatments in a randomized complete block design with a 2×2 factorial arrangement of treatments	817:925	After a 4-week preliminary period, cows were assigned to one of four topdress treatments in a randomized complete block design with a 2×2 factorial arrangement of treatments: (1) NRC inorganic (NRC levels using inorganic (sulfate-based) trace mineral supplements only); (2) NRC organic (NRC levels using organic trace mineral supplements (metals chelated to 2-hydroxy-4-(methythio)-butanoic acid); (3) commercial inorganic (approximately 2×NRC levels using inorganic trace mineral supplements only; and (4) commercial organic (commercial levels using organic trace mineral supplements only).
30322418	10	105	theme	organic	2285:2291	arg1	sources					2293:2299	organic sources	2285:2299	organic sources	2285:2299	Cows were administered an intramammary LPS challenge during week 5; during week 6 cows fed commercial levels of Zn, Cu and Mn tended to have higher plasma TAC and cows fed organic sources had decreased plasma TBARS.
30322418	4	106	theme	randomized	847:856	arg1	design					873:878	a randomized complete block design	845:878	a randomized complete block design with a 2×2 factorial arrangement of treatments	845:925	After a 4-week preliminary period, cows were assigned to one of four topdress treatments in a randomized complete block design with a 2×2 factorial arrangement of treatments: (1) NRC inorganic (NRC levels using inorganic (sulfate-based) trace mineral supplements only); (2) NRC organic (NRC levels using organic trace mineral supplements (metals chelated to 2-hydroxy-4-(methythio)-butanoic acid); (3) commercial inorganic (approximately 2×NRC levels using inorganic trace mineral supplements only; and (4) commercial organic (commercial levels using organic trace mineral supplements only).
30322418	9	107	theme	Escherichia	2009:2019	arg1	vaccination					2038:2048	Escherichia coli J5 bacterin vaccination	2009:2048	Escherichia coli J5 bacterin vaccination	2009:2048	Plasma immunoglobulin G level was higher in cows supplemented with organic trace minerals over the entire treatment period; responses assessed as differences of before and after Escherichia coli J5 bacterin vaccination at the end of week 2 of treatment period were not significant.
30322418	4	108	theme	topdress	822:829	arg1	treatments					831:840	four topdress treatments	817:840	four topdress treatments in a randomized complete block design with a 2×2 factorial arrangement of treatments	817:925	After a 4-week preliminary period, cows were assigned to one of four topdress treatments in a randomized complete block design with a 2×2 factorial arrangement of treatments: (1) NRC inorganic (NRC levels using inorganic (sulfate-based) trace mineral supplements only); (2) NRC organic (NRC levels using organic trace mineral supplements (metals chelated to 2-hydroxy-4-(methythio)-butanoic acid); (3) commercial inorganic (approximately 2×NRC levels using inorganic trace mineral supplements only; and (4) commercial organic (commercial levels using organic trace mineral supplements only).
30322418	9	109	theme	entire	1930:1935	arg1	period					1947:1952	the entire treatment period	1926:1952	the entire treatment period	1926:1952	Plasma immunoglobulin G level was higher in cows supplemented with organic trace minerals over the entire treatment period; responses assessed as differences of before and after Escherichia coli J5 bacterin vaccination at the end of week 2 of treatment period were not significant.
30322418	7	110	theme	reactive	1593:1600	arg1	TBARS					1614:1618	TBARS	1614:1618	TBARS	1614:1618	Activities of super oxide dismutase and glutathione peroxidase in erythrocyte lysate and concentrations of thiobarbituric acid reactive substances (TBARS) and total antioxidant capacity (TAC) in plasma were measured as indices of oxidative metabolism.
30322418	7	110	theme	reactive	1593:1600	arg1	substances					1602:1611	thiobarbituric acid reactive substances	1573:1611	thiobarbituric acid reactive substances (TBARS)	1573:1619	Activities of super oxide dismutase and glutathione peroxidase in erythrocyte lysate and concentrations of thiobarbituric acid reactive substances (TBARS) and total antioxidant capacity (TAC) in plasma were measured as indices of oxidative metabolism.
30322418	13	111	theme	oxidative	2778:2786	arg1	metabolism					2788:2797	oxidative metabolism indices and clinical responses to intramammary LPS challenge in midlactation cows	2778:2879	metabolism	2788:2797	Overall, results suggest that the varying level and source of dietary trace minerals do not have significant short-term effects on oxidative metabolism indices and clinical responses to intramammary LPS challenge in midlactation cows.
30322418	2	112	theme	metabolism	534:543	arg1	responses					549:557	responses	549:557	responses to intramammary lipopolysaccharide (LPS) challenge	549:608	Multiparous Holstein cows (n=48) beginning at 60 to 140 days in milk were utilized to determine the effects of trace mineral amount and source on aspects of oxidative metabolism and responses to intramammary lipopolysaccharide (LPS) challenge.
30322418	2	112	theme	metabolism	534:543	arg1	aspects					513:519	aspects	513:519	aspects of oxidative metabolism	513:543	Multiparous Holstein cows (n=48) beginning at 60 to 140 days in milk were utilized to determine the effects of trace mineral amount and source on aspects of oxidative metabolism and responses to intramammary lipopolysaccharide (LPS) challenge.
30322418	10	113	theme	plasma	2315:2320	arg1	TBARS					2322:2326	decreased plasma TBARS	2305:2326	decreased plasma TBARS	2305:2326	Cows were administered an intramammary LPS challenge during week 5; during week 6 cows fed commercial levels of Zn, Cu and Mn tended to have higher plasma TAC and cows fed organic sources had decreased plasma TBARS.
30322418	10	114	dep	fed	2281:2283	arg1	had					2301:2303	had	2301:2303	fed organic sources had decreased plasma TBARS	2281:2326	Cows were administered an intramammary LPS challenge during week 5; during week 6 cows fed commercial levels of Zn, Cu and Mn tended to have higher plasma TAC and cows fed organic sources had decreased plasma TBARS.
30322418	7	115	theme	thiobarbituric	1573:1586	arg1	TBARS					1614:1618	TBARS	1614:1618	TBARS	1614:1618	Activities of super oxide dismutase and glutathione peroxidase in erythrocyte lysate and concentrations of thiobarbituric acid reactive substances (TBARS) and total antioxidant capacity (TAC) in plasma were measured as indices of oxidative metabolism.
30322418	7	115	theme	thiobarbituric	1573:1586	arg1	substances					1602:1611	thiobarbituric acid reactive substances	1573:1611	thiobarbituric acid reactive substances (TBARS)	1573:1619	Activities of super oxide dismutase and glutathione peroxidase in erythrocyte lysate and concentrations of thiobarbituric acid reactive substances (TBARS) and total antioxidant capacity (TAC) in plasma were measured as indices of oxidative metabolism.
30322418	9	116	theme	Plasma	1831:1836	arg1	G					1853:1853	Plasma immunoglobulin G	1831:1853	Plasma immunoglobulin G level	1831:1859	Plasma immunoglobulin G level was higher in cows supplemented with organic trace minerals over the entire treatment period; responses assessed as differences of before and after Escherichia coli J5 bacterin vaccination at the end of week 2 of treatment period were not significant.
30322418	0	117	theme	metabolism	67:76	arg1	responses					82:90	responses	82:90	responses to intramammary lipopolysaccharide challenge in midlactation dairy cows	82:162	Effects of trace mineral amount and source on aspects of oxidative metabolism and responses to intramammary lipopolysaccharide challenge in midlactation dairy cows.
30322418	0	117	theme	metabolism	67:76	arg1	aspects					46:52	aspects	46:52	aspects of oxidative metabolism	46:76	Effects of trace mineral amount and source on aspects of oxidative metabolism and responses to intramammary lipopolysaccharide challenge in midlactation dairy cows.
30322418	13	118	theme	significant	2744:2754	arg1	effects					2767:2773	significant short-term effects	2744:2773	significant short-term effects	2744:2773	Overall, results suggest that the varying level and source of dietary trace minerals do not have significant short-term effects on oxidative metabolism indices and clinical responses to intramammary LPS challenge in midlactation cows.
30322418	13	119	theme	dietary	2709:2715	arg1	minerals					2723:2730	dietary trace minerals	2709:2730	dietary trace minerals	2709:2730	Overall, results suggest that the varying level and source of dietary trace minerals do not have significant short-term effects on oxidative metabolism indices and clinical responses to intramammary LPS challenge in midlactation cows.
30322418	9	120	theme	G	1853:1853	arg1	level					1855:1859	Plasma immunoglobulin G level	1831:1859	Plasma immunoglobulin G level	1831:1859	Plasma immunoglobulin G level was higher in cows supplemented with organic trace minerals over the entire treatment period; responses assessed as differences of before and after Escherichia coli J5 bacterin vaccination at the end of week 2 of treatment period were not significant.
30322418	9	121	theme	treatment	2074:2082	arg1	period					2084:2089	treatment period	2074:2089	treatment period	2074:2089	Plasma immunoglobulin G level was higher in cows supplemented with organic trace minerals over the entire treatment period; responses assessed as differences of before and after Escherichia coli J5 bacterin vaccination at the end of week 2 of treatment period were not significant.
30322418	1	122	theme	dairy	353:357	arg1	cattle					359:364	dairy cattle	353:364	dairy cattle	353:364	Trace minerals have important roles in immune function and oxidative metabolism; however, little is known about the relationships between supplementation level and source with outcomes in dairy cattle.
30322418	10	123	theme	Zn	2225:2226	arg1	levels					2215:2220	commercial levels	2204:2220	commercial levels of Zn, Cu and Mn	2204:2237	Cows were administered an intramammary LPS challenge during week 5; during week 6 cows fed commercial levels of Zn, Cu and Mn tended to have higher plasma TAC and cows fed organic sources had decreased plasma TBARS.
30322418	0	124	theme	intramammary	95:106	arg1	challenge					127:135	intramammary lipopolysaccharide challenge	95:135	intramammary lipopolysaccharide challenge in midlactation dairy cows	95:162	Effects of trace mineral amount and source on aspects of oxidative metabolism and responses to intramammary lipopolysaccharide challenge in midlactation dairy cows.
30322418	4	125	dep	NRC	932:934	arg1	1					929:929	1	929:929	1	929:929	After a 4-week preliminary period, cows were assigned to one of four topdress treatments in a randomized complete block design with a 2×2 factorial arrangement of treatments: (1) NRC inorganic (NRC levels using inorganic (sulfate-based) trace mineral supplements only); (2) NRC organic (NRC levels using organic trace mineral supplements (metals chelated to 2-hydroxy-4-(methythio)-butanoic acid); (3) commercial inorganic (approximately 2×NRC levels using inorganic trace mineral supplements only; and (4) commercial organic (commercial levels using organic trace mineral supplements only).
30322418	4	126	theme	complete	858:865	arg1	design					873:878	a randomized complete block design	845:878	a randomized complete block design with a 2×2 factorial arrangement of treatments	845:925	After a 4-week preliminary period, cows were assigned to one of four topdress treatments in a randomized complete block design with a 2×2 factorial arrangement of treatments: (1) NRC inorganic (NRC levels using inorganic (sulfate-based) trace mineral supplements only); (2) NRC organic (NRC levels using organic trace mineral supplements (metals chelated to 2-hydroxy-4-(methythio)-butanoic acid); (3) commercial inorganic (approximately 2×NRC levels using inorganic trace mineral supplements only; and (4) commercial organic (commercial levels using organic trace mineral supplements only).
30322418	1	127	dep	supplementation	303:317	arg1	level					319:323	level	319:323	level	319:323	Trace minerals have important roles in immune function and oxidative metabolism; however, little is known about the relationships between supplementation level and source with outcomes in dairy cattle.
30322418	4	128	theme	organic	1057:1063	arg1	supplements					1079:1089	organic trace mineral supplements	1057:1089	organic trace mineral supplements (metals chelated to 2-hydroxy-4-(methythio)-butanoic acid)	1057:1148	After a 4-week preliminary period, cows were assigned to one of four topdress treatments in a randomized complete block design with a 2×2 factorial arrangement of treatments: (1) NRC inorganic (NRC levels using inorganic (sulfate-based) trace mineral supplements only); (2) NRC organic (NRC levels using organic trace mineral supplements (metals chelated to 2-hydroxy-4-(methythio)-butanoic acid); (3) commercial inorganic (approximately 2×NRC levels using inorganic trace mineral supplements only; and (4) commercial organic (commercial levels using organic trace mineral supplements only).
30322418	4	129	theme	block	867:871	arg1	design					873:878	a randomized complete block design	845:878	a randomized complete block design with a 2×2 factorial arrangement of treatments	845:925	After a 4-week preliminary period, cows were assigned to one of four topdress treatments in a randomized complete block design with a 2×2 factorial arrangement of treatments: (1) NRC inorganic (NRC levels using inorganic (sulfate-based) trace mineral supplements only); (2) NRC organic (NRC levels using organic trace mineral supplements (metals chelated to 2-hydroxy-4-(methythio)-butanoic acid); (3) commercial inorganic (approximately 2×NRC levels using inorganic trace mineral supplements only; and (4) commercial organic (commercial levels using organic trace mineral supplements only).
30322418	12	130	theme	matter	2570:2575	arg1	intake					2577:2582	dry matter intake	2566:2582	dry matter intake	2566:2582	Productive performance including dry matter intake and milk yield and composition were not affected by treatment.
30322418	7	131	theme	glutathione	1506:1516	arg1	peroxidase					1518:1527	glutathione peroxidase	1506:1527	glutathione peroxidase	1506:1527	Activities of super oxide dismutase and glutathione peroxidase in erythrocyte lysate and concentrations of thiobarbituric acid reactive substances (TBARS) and total antioxidant capacity (TAC) in plasma were measured as indices of oxidative metabolism.
30322418	10	132	theme	Mn	2236:2237	arg1	levels					2215:2220	commercial levels	2204:2220	commercial levels of Zn, Cu and Mn	2204:2237	Cows were administered an intramammary LPS challenge during week 5; during week 6 cows fed commercial levels of Zn, Cu and Mn tended to have higher plasma TAC and cows fed organic sources had decreased plasma TBARS.
30322418	4	133	theme	mineral	1071:1077	arg1	supplements					1079:1089	organic trace mineral supplements	1057:1089	organic trace mineral supplements (metals chelated to 2-hydroxy-4-(methythio)-butanoic acid)	1057:1148	After a 4-week preliminary period, cows were assigned to one of four topdress treatments in a randomized complete block design with a 2×2 factorial arrangement of treatments: (1) NRC inorganic (NRC levels using inorganic (sulfate-based) trace mineral supplements only); (2) NRC organic (NRC levels using organic trace mineral supplements (metals chelated to 2-hydroxy-4-(methythio)-butanoic acid); (3) commercial inorganic (approximately 2×NRC levels using inorganic trace mineral supplements only; and (4) commercial organic (commercial levels using organic trace mineral supplements only).
30322418	0	134	theme	mineral	17:23	arg1	amount					25:30	trace mineral amount	11:30	trace mineral amount	11:30	Effects of trace mineral amount and source on aspects of oxidative metabolism and responses to intramammary lipopolysaccharide challenge in midlactation dairy cows.
30322418	7	135	theme	dismutase	1492:1500	arg1	indices					1685:1691	indices	1685:1691	indices of oxidative metabolism	1685:1715	Activities of super oxide dismutase and glutathione peroxidase in erythrocyte lysate and concentrations of thiobarbituric acid reactive substances (TBARS) and total antioxidant capacity (TAC) in plasma were measured as indices of oxidative metabolism.
30322418	7	135	theme	dismutase	1492:1500	arg1	concentrations					1555:1568	concentrations	1555:1568	concentrations of thiobarbituric acid reactive substances (TBARS) and total antioxidant capacity (TAC) in plasma	1555:1666	Activities of super oxide dismutase and glutathione peroxidase in erythrocyte lysate and concentrations of thiobarbituric acid reactive substances (TBARS) and total antioxidant capacity (TAC) in plasma were measured as indices of oxidative metabolism.
30322418	7	135	theme	dismutase	1492:1500	arg1	Activities					1466:1475	Activities	1466:1475	Activities of super oxide dismutase and glutathione peroxidase in erythrocyte lysate	1466:1549	Activities of super oxide dismutase and glutathione peroxidase in erythrocyte lysate and concentrations of thiobarbituric acid reactive substances (TBARS) and total antioxidant capacity (TAC) in plasma were measured as indices of oxidative metabolism.
30322418	10	136	theme	Cu	2229:2230	arg1	levels					2215:2220	commercial levels	2204:2220	commercial levels of Zn, Cu and Mn	2204:2237	Cows were administered an intramammary LPS challenge during week 5; during week 6 cows fed commercial levels of Zn, Cu and Mn tended to have higher plasma TAC and cows fed organic sources had decreased plasma TBARS.
30322418	11	137	theme	cortisol	2399:2406	arg1	concentrations					2408:2421	plasma cortisol concentrations	2392:2421	plasma cortisol concentrations	2392:2421	After the LPS challenge, the extent and pattern of response of plasma cortisol concentrations and clinical indices (rectal temperature and heart rate) were not affected by trace mineral level and source.
30322418	4	138	theme	-butanoic	1134:1142	arg1	acid					1144:1147	2-hydroxy-4-(methythio)-butanoic acid	1111:1147	2-hydroxy-4-(methythio)-butanoic acid	1111:1147	After a 4-week preliminary period, cows were assigned to one of four topdress treatments in a randomized complete block design with a 2×2 factorial arrangement of treatments: (1) NRC inorganic (NRC levels using inorganic (sulfate-based) trace mineral supplements only); (2) NRC organic (NRC levels using organic trace mineral supplements (metals chelated to 2-hydroxy-4-(methythio)-butanoic acid); (3) commercial inorganic (approximately 2×NRC levels using inorganic trace mineral supplements only; and (4) commercial organic (commercial levels using organic trace mineral supplements only).
30322418	4	139	theme	commercial	1280:1289	arg1	levels					1291:1296	commercial levels	1280:1296	commercial levels using organic trace mineral supplements only	1280:1341	After a 4-week preliminary period, cows were assigned to one of four topdress treatments in a randomized complete block design with a 2×2 factorial arrangement of treatments: (1) NRC inorganic (NRC levels using inorganic (sulfate-based) trace mineral supplements only); (2) NRC organic (NRC levels using organic trace mineral supplements (metals chelated to 2-hydroxy-4-(methythio)-butanoic acid); (3) commercial inorganic (approximately 2×NRC levels using inorganic trace mineral supplements only; and (4) commercial organic (commercial levels using organic trace mineral supplements only).
30322418	11	140	dep	extent	2358:2363	arg1	the					2354:2356	the	2354:2356	the	2354:2356	After the LPS challenge, the extent and pattern of response of plasma cortisol concentrations and clinical indices (rectal temperature and heart rate) were not affected by trace mineral level and source.
30322418	7	141	theme	super	1480:1484	arg1	dismutase					1492:1500	super oxide dismutase	1480:1500	super oxide dismutase	1480:1500	Activities of super oxide dismutase and glutathione peroxidase in erythrocyte lysate and concentrations of thiobarbituric acid reactive substances (TBARS) and total antioxidant capacity (TAC) in plasma were measured as indices of oxidative metabolism.
30322418	4	142	theme	factorial	891:899	arg1	arrangement					901:911	a 2×2 factorial arrangement	885:911	a 2×2 factorial arrangement of treatments	885:925	After a 4-week preliminary period, cows were assigned to one of four topdress treatments in a randomized complete block design with a 2×2 factorial arrangement of treatments: (1) NRC inorganic (NRC levels using inorganic (sulfate-based) trace mineral supplements only); (2) NRC organic (NRC levels using organic trace mineral supplements (metals chelated to 2-hydroxy-4-(methythio)-butanoic acid); (3) commercial inorganic (approximately 2×NRC levels using inorganic trace mineral supplements only; and (4) commercial organic (commercial levels using organic trace mineral supplements only).
30322418	11	143	theme	rectal	2445:2450	arg1	indices					2436:2442	clinical indices	2427:2442	clinical indices (rectal temperature and heart rate)	2427:2478	After the LPS challenge, the extent and pattern of response of plasma cortisol concentrations and clinical indices (rectal temperature and heart rate) were not affected by trace mineral level and source.
30322418	11	143	theme	rectal	2445:2450	arg1	temperature					2452:2462	rectal temperature	2445:2462	rectal temperature	2445:2462	After the LPS challenge, the extent and pattern of response of plasma cortisol concentrations and clinical indices (rectal temperature and heart rate) were not affected by trace mineral level and source.
30322418	5	144	theme	mineral	1374:1380	arg1	treatments					1382:1391	the respective mineral treatments	1359:1391	the respective mineral treatments	1359:1391	Cows were fed the respective mineral treatments for 6 weeks.
30322418	1	145	contain	have	180:183	arg1	minerals					171:178	Trace minerals	165:178	Trace minerals	165:178	Trace minerals have important roles in immune function and oxidative metabolism; however, little is known about the relationships between supplementation level and source with outcomes in dairy cattle.
30322418	1	145	contain	have	180:183	arg2	roles					195:199	important roles	185:199	important roles	185:199	Trace minerals have important roles in immune function and oxidative metabolism; however, little is known about the relationships between supplementation level and source with outcomes in dairy cattle.
30322418	8	146	theme	experimental	1810:1821	arg1	period					1823:1828	the entire experimental period	1799:1828	the entire experimental period	1799:1828	Effects of treatment on those indices were not significant when evaluated across the entire experimental period.
30322418	2	147	theme	mineral	484:490	arg1	amount					492:497	trace mineral amount	478:497	trace mineral amount	478:497	Multiparous Holstein cows (n=48) beginning at 60 to 140 days in milk were utilized to determine the effects of trace mineral amount and source on aspects of oxidative metabolism and responses to intramammary lipopolysaccharide (LPS) challenge.
30322418	7	148	theme	oxidative	1696:1704	arg1	metabolism					1706:1715	oxidative metabolism	1696:1715	oxidative metabolism	1696:1715	Activities of super oxide dismutase and glutathione peroxidase in erythrocyte lysate and concentrations of thiobarbituric acid reactive substances (TBARS) and total antioxidant capacity (TAC) in plasma were measured as indices of oxidative metabolism.
30322418	10	149	theme	LPS	2152:2154	arg1	challenge					2156:2164	an intramammary LPS challenge	2136:2164	an intramammary LPS challenge	2136:2164	Cows were administered an intramammary LPS challenge during week 5; during week 6 cows fed commercial levels of Zn, Cu and Mn tended to have higher plasma TAC and cows fed organic sources had decreased plasma TBARS.
30322418	8	150	from	Effects	1718:1724	arg1	indices					1748:1754	those indices	1742:1754	those indices	1742:1754	Effects of treatment on those indices were not significant when evaluated across the entire experimental period.
30322418	3	151	theme	National	646:653	arg1	NRC					673:675	NRC	673:675	NRC	673:675	Cows were fed a basal diet meeting National Research Council (NRC) requirements except for no added zinc (Zn), copper (Cu) or manganese (Mn).
30322418	3	151	theme	National	646:653	arg1	Council					664:670	National Research Council	646:670	National Research Council (NRC) requirements	646:689	Cows were fed a basal diet meeting National Research Council (NRC) requirements except for no added zinc (Zn), copper (Cu) or manganese (Mn).
30322418	12	152	theme	dry	2566:2568	arg1	intake					2577:2582	dry matter intake	2566:2582	dry matter intake	2566:2582	Productive performance including dry matter intake and milk yield and composition were not affected by treatment.
30322418	4	153	dep	organic	1271:1277	arg1	levels					1291:1296	commercial levels	1280:1296	commercial levels using organic trace mineral supplements only	1280:1341	After a 4-week preliminary period, cows were assigned to one of four topdress treatments in a randomized complete block design with a 2×2 factorial arrangement of treatments: (1) NRC inorganic (NRC levels using inorganic (sulfate-based) trace mineral supplements only); (2) NRC organic (NRC levels using organic trace mineral supplements (metals chelated to 2-hydroxy-4-(methythio)-butanoic acid); (3) commercial inorganic (approximately 2×NRC levels using inorganic trace mineral supplements only; and (4) commercial organic (commercial levels using organic trace mineral supplements only).
30322418	4	154	with	design	873:878	arg1	arrangement					901:911	a 2×2 factorial arrangement	885:911	a 2×2 factorial arrangement of treatments	885:925	After a 4-week preliminary period, cows were assigned to one of four topdress treatments in a randomized complete block design with a 2×2 factorial arrangement of treatments: (1) NRC inorganic (NRC levels using inorganic (sulfate-based) trace mineral supplements only); (2) NRC organic (NRC levels using organic trace mineral supplements (metals chelated to 2-hydroxy-4-(methythio)-butanoic acid); (3) commercial inorganic (approximately 2×NRC levels using inorganic trace mineral supplements only; and (4) commercial organic (commercial levels using organic trace mineral supplements only).
30322418	9	155	theme	treatment	1937:1945	arg1	period					1947:1952	the entire treatment period	1926:1952	the entire treatment period	1926:1952	Plasma immunoglobulin G level was higher in cows supplemented with organic trace minerals over the entire treatment period; responses assessed as differences of before and after Escherichia coli J5 bacterin vaccination at the end of week 2 of treatment period were not significant.
30322418	13	156	theme	trace	2717:2721	arg1	minerals					2723:2730	dietary trace minerals	2709:2730	dietary trace minerals	2709:2730	Overall, results suggest that the varying level and source of dietary trace minerals do not have significant short-term effects on oxidative metabolism indices and clinical responses to intramammary LPS challenge in midlactation cows.
30322418	4	157	theme	inorganic	964:972	arg1	supplements					1004:1014	inorganic (sulfate-based) trace mineral supplements	964:1014	inorganic (sulfate-based) trace mineral supplements only	964:1019	After a 4-week preliminary period, cows were assigned to one of four topdress treatments in a randomized complete block design with a 2×2 factorial arrangement of treatments: (1) NRC inorganic (NRC levels using inorganic (sulfate-based) trace mineral supplements only); (2) NRC organic (NRC levels using organic trace mineral supplements (metals chelated to 2-hydroxy-4-(methythio)-butanoic acid); (3) commercial inorganic (approximately 2×NRC levels using inorganic trace mineral supplements only; and (4) commercial organic (commercial levels using organic trace mineral supplements only).
30322418	0	158	from	challenge	127:135	arg1	cows					159:162	midlactation dairy cows	140:162	midlactation dairy cows	140:162	Effects of trace mineral amount and source on aspects of oxidative metabolism and responses to intramammary lipopolysaccharide challenge in midlactation dairy cows.
30322418	2	159	theme	Multiparous	367:377	arg1	n=48					394:397	n=48	394:397	n=48	394:397	Multiparous Holstein cows (n=48) beginning at 60 to 140 days in milk were utilized to determine the effects of trace mineral amount and source on aspects of oxidative metabolism and responses to intramammary lipopolysaccharide (LPS) challenge.
30322418	2	159	theme	Multiparous	367:377	arg1	cows					388:391	Multiparous Holstein cows	367:391	Multiparous Holstein cows (n=48) beginning at 60 to 140 days in milk	367:434	Multiparous Holstein cows (n=48) beginning at 60 to 140 days in milk were utilized to determine the effects of trace mineral amount and source on aspects of oxidative metabolism and responses to intramammary lipopolysaccharide (LPS) challenge.
29443967	9	0	theme	flippase	1655:1662	arg1	MurJ					1664:1667	the putative lipid II flippase MurJ	1633:1667	the putative lipid II flippase MurJ	1633:1667	Here we investigate the localization of S. aureus peptidoglycan biosynthesis proteins and show that the recruitment of the putative lipid II flippase MurJ to the septum, by the DivIB-DivIC-FtsL complex, drives peptidoglycan incorporation to the midcell.
29443967	7	1	theme	cell	1383:1386	arg1	periphery					1388:1396	the cell periphery	1379:1396	the cell periphery	1379:1396	Cocci such as Staphylococcus aureus possess only one type of peptidoglycan synthesis machinery, which is diverted from the cell periphery to the septum in preparation for division.
29443967	9	2	theme	proteins	1591:1598	arg1	localization					1538:1549	the localization	1534:1549	the localization of S. aureus peptidoglycan biosynthesis proteins	1534:1598	Here we investigate the localization of S. aureus peptidoglycan biosynthesis proteins and show that the recruitment of the putative lipid II flippase MurJ to the septum, by the DivIB-DivIC-FtsL complex, drives peptidoglycan incorporation to the midcell.
29443967	3	3	theme	cytoskeletal	696:707	arg1	FtsZ					718:721	FtsZ	718:721	FtsZ	718:721	The form of rod-shaped bacteria, such as Bacillus subtilis or Escherichia coli, is generated by the action of two peptidoglycan synthesis machineries that act at the septum and at the lateral wall in processes coordinated by the cytoskeletal proteins FtsZ and MreB, respectively.
29443967	3	3	theme	cytoskeletal	696:707	arg1	proteins					709:716	the cytoskeletal proteins	692:716	the cytoskeletal proteins FtsZ and MreB	692:730	The form of rod-shaped bacteria, such as Bacillus subtilis or Escherichia coli, is generated by the action of two peptidoglycan synthesis machineries that act at the septum and at the lateral wall in processes coordinated by the cytoskeletal proteins FtsZ and MreB, respectively.
29443967	3	3	theme	cytoskeletal	696:707	arg1	MreB					727:730	MreB	727:730	MreB	727:730	The form of rod-shaped bacteria, such as Bacillus subtilis or Escherichia coli, is generated by the action of two peptidoglycan synthesis machineries that act at the septum and at the lateral wall in processes coordinated by the cytoskeletal proteins FtsZ and MreB, respectively.
29443967	4	4	theme	Z	871:871	arg1	ring-that					873:881	the Z ring-that	867:881	the Z ring-that undergo treadmilling and recruit later divisome proteins	867:938	The tubulin homologue FtsZ is the first protein recruited to the division site, where it assembles in filaments-forming the Z ring-that undergo treadmilling and recruit later divisome proteins.
29443967	1	5	theme	mechanical	171:180	arg1	stress					182:187	the mechanical stress	167:187	the mechanical stress that results from high intracellular turgor	167:231	Peptidoglycan is the main component of the bacterial wall and protects cells from the mechanical stress that results from high intracellular turgor.
29443967	5	6	from	rate	945:948	arg1	subtilis					972:979	B. subtilis	969:979	B. subtilis	969:979	The rate of treadmilling in B. subtilis controls the rates of both peptidoglycan synthesis and cell division.
29443967	5	7	theme	division	1041:1048	arg1	rates					994:998	the rates	990:998	the rates of both peptidoglycan synthesis and cell division	990:1048	The rate of treadmilling in B. subtilis controls the rates of both peptidoglycan synthesis and cell division.
29443967	10	8	theme	turning	1802:1808	arg1	point					1810:1814	a turning point	1800:1814	a turning point	1800:1814	MurJ recruitment corresponds to a turning point in cytokinesis, which is slow and dependent on FtsZ treadmilling before MurJ arrival but becomes faster and independent of FtsZ treadmilling after peptidoglycan synthesis activity is directed to the septum, where it provides additional force for cell envelope constriction.
29443967	10	8	theme	turning	1802:1808	arg1	slow					1841:1844	slow	1841:1844	slow	1841:1844	MurJ recruitment corresponds to a turning point in cytokinesis, which is slow and dependent on FtsZ treadmilling before MurJ arrival but becomes faster and independent of FtsZ treadmilling after peptidoglycan synthesis activity is directed to the septum, where it provides additional force for cell envelope constriction.
29443967	1	9	theme	main	106:109	arg1	component					111:119	the main component	102:119	the main component of the bacterial wall	102:141	Peptidoglycan is the main component of the bacterial wall and protects cells from the mechanical stress that results from high intracellular turgor.
29443967	1	9	theme	main	106:109	arg1	Peptidoglycan					85:97	Peptidoglycan	85:97	Peptidoglycan	85:97	Peptidoglycan is the main component of the bacterial wall and protects cells from the mechanical stress that results from high intracellular turgor.
29443967	6	10	theme	long	1174:1177	arg1	axis					1179:1182	the long axis	1170:1182	the long axis of the cell	1170:1194	The actin homologue MreB forms discrete patches that move circumferentially around the cell in tracks perpendicular to the long axis of the cell, and organize the insertion of new cell wall during elongation.
29443967	9	11	theme	S.	1554:1555	arg1	proteins					1591:1598	S. aureus peptidoglycan biosynthesis proteins	1554:1598	S. aureus peptidoglycan biosynthesis proteins	1554:1598	Here we investigate the localization of S. aureus peptidoglycan biosynthesis proteins and show that the recruitment of the putative lipid II flippase MurJ to the septum, by the DivIB-DivIC-FtsL complex, drives peptidoglycan incorporation to the midcell.
29443967	6	12	theme	cell	1191:1194	arg1	axis					1179:1182	the long axis	1170:1182	the long axis of the cell	1170:1194	The actin homologue MreB forms discrete patches that move circumferentially around the cell in tracks perpendicular to the long axis of the cell, and organize the insertion of new cell wall during elongation.
29443967	10	13	theme	MurJ	1768:1771	arg1	recruitment					1773:1783	MurJ recruitment	1768:1783	MurJ recruitment	1768:1783	MurJ recruitment corresponds to a turning point in cytokinesis, which is slow and dependent on FtsZ treadmilling before MurJ arrival but becomes faster and independent of FtsZ treadmilling after peptidoglycan synthesis activity is directed to the septum, where it provides additional force for cell envelope constriction.
29443967	3	14	from	wall	659:662	arg1	processes					667:675	processes	667:675	processes coordinated by the cytoskeletal proteins FtsZ and MreB, respectively	667:744	The form of rod-shaped bacteria, such as Bacillus subtilis or Escherichia coli, is generated by the action of two peptidoglycan synthesis machineries that act at the septum and at the lateral wall in processes coordinated by the cytoskeletal proteins FtsZ and MreB, respectively.
29443967	1	15	theme	bacterial	128:136	arg1	wall					138:141	the bacterial wall	124:141	the bacterial wall	124:141	Peptidoglycan is the main component of the bacterial wall and protects cells from the mechanical stress that results from high intracellular turgor.
29443967	4	16	theme	tubulin	751:757	arg1	FtsZ					769:772	The tubulin homologue FtsZ	747:772	The tubulin homologue FtsZ	747:772	The tubulin homologue FtsZ is the first protein recruited to the division site, where it assembles in filaments-forming the Z ring-that undergo treadmilling and recruit later divisome proteins.
29443967	4	16	theme	tubulin	751:757	arg1	protein					787:793	the first protein	777:793	the first protein recruited to the division site, where it assembles in filaments-forming the Z ring-that undergo treadmilling and recruit later divisome proteins	777:938	The tubulin homologue FtsZ is the first protein recruited to the division site, where it assembles in filaments-forming the Z ring-that undergo treadmilling and recruit later divisome proteins.
29443967	2	17	theme	bacterial	294:302	arg1	shapes					304:309	bacterial shapes	294:309	bacterial shapes	294:309	Peptidoglycan biosynthesis is very similar in all bacteria; bacterial shapes are therefore mainly determined by the spatial and temporal regulation of peptidoglycan synthesis rather than by the chemical composition of peptidoglycan.
29443967	7	18	from	septum	1405:1410	arg1	preparation					1415:1425	preparation	1415:1425	preparation for division	1415:1438	Cocci such as Staphylococcus aureus possess only one type of peptidoglycan synthesis machinery, which is diverted from the cell periphery to the septum in preparation for division.
29443967	6	19	theme	homologue	1061:1069	arg1	MreB					1071:1074	The actin homologue MreB	1051:1074	The actin homologue MreB	1051:1074	The actin homologue MreB forms discrete patches that move circumferentially around the cell in tracks perpendicular to the long axis of the cell, and organize the insertion of new cell wall during elongation.
29443967	10	20	theme	additional	2041:2050	arg1	force					2052:2056	additional force	2041:2056	additional force for cell envelope constriction	2041:2087	MurJ recruitment corresponds to a turning point in cytokinesis, which is slow and dependent on FtsZ treadmilling before MurJ arrival but becomes faster and independent of FtsZ treadmilling after peptidoglycan synthesis activity is directed to the septum, where it provides additional force for cell envelope constriction.
29443967	10	21	theme	FtsZ	1939:1942	arg1	treadmilling					1944:1955	FtsZ treadmilling	1939:1955	FtsZ treadmilling	1939:1955	MurJ recruitment corresponds to a turning point in cytokinesis, which is slow and dependent on FtsZ treadmilling before MurJ arrival but becomes faster and independent of FtsZ treadmilling after peptidoglycan synthesis activity is directed to the septum, where it provides additional force for cell envelope constriction.
29443967	10	22	theme	cell	2062:2065	arg1	constriction					2076:2087	cell envelope constriction	2062:2087	cell envelope constriction	2062:2087	MurJ recruitment corresponds to a turning point in cytokinesis, which is slow and dependent on FtsZ treadmilling before MurJ arrival but becomes faster and independent of FtsZ treadmilling after peptidoglycan synthesis activity is directed to the septum, where it provides additional force for cell envelope constriction.
29443967	4	23	theme	first	781:785	arg1	FtsZ					769:772	The tubulin homologue FtsZ	747:772	The tubulin homologue FtsZ	747:772	The tubulin homologue FtsZ is the first protein recruited to the division site, where it assembles in filaments-forming the Z ring-that undergo treadmilling and recruit later divisome proteins.
29443967	4	23	theme	first	781:785	arg1	protein					787:793	the first protein	777:793	the first protein recruited to the division site, where it assembles in filaments-forming the Z ring-that undergo treadmilling and recruit later divisome proteins	777:938	The tubulin homologue FtsZ is the first protein recruited to the division site, where it assembles in filaments-forming the Z ring-that undergo treadmilling and recruit later divisome proteins.
29443967	7	24	theme	machinery	1345:1353	arg1	type					1313:1316	only one type	1304:1316	only one type	1304:1316	Cocci such as Staphylococcus aureus possess only one type of peptidoglycan synthesis machinery, which is diverted from the cell periphery to the septum in preparation for division.
29443967	10	25	theme	peptidoglycan	1963:1975	arg1	activity					1987:1994	peptidoglycan synthesis activity	1963:1994	peptidoglycan synthesis activity	1963:1994	MurJ recruitment corresponds to a turning point in cytokinesis, which is slow and dependent on FtsZ treadmilling before MurJ arrival but becomes faster and independent of FtsZ treadmilling after peptidoglycan synthesis activity is directed to the septum, where it provides additional force for cell envelope constriction.
29443967	9	26	theme	MurJ	1664:1667	arg1	recruitment					1618:1628	the recruitment	1614:1628	the recruitment of the putative lipid II flippase MurJ to the septum	1614:1681	Here we investigate the localization of S. aureus peptidoglycan biosynthesis proteins and show that the recruitment of the putative lipid II flippase MurJ to the septum, by the DivIB-DivIC-FtsL complex, drives peptidoglycan incorporation to the midcell.
29443967	5	27	theme	peptidoglycan	1008:1020	arg1	synthesis					1022:1030	peptidoglycan synthesis	1008:1030	peptidoglycan synthesis	1008:1030	The rate of treadmilling in B. subtilis controls the rates of both peptidoglycan synthesis and cell division.
29443967	3	28	theme	peptidoglycan	581:593	arg1	machineries					605:615	two peptidoglycan synthesis machineries	577:615	two peptidoglycan synthesis machineries that act at the septum and at the lateral wall in processes coordinated by the cytoskeletal proteins FtsZ and MreB, respectively	577:744	The form of rod-shaped bacteria, such as Bacillus subtilis or Escherichia coli, is generated by the action of two peptidoglycan synthesis machineries that act at the septum and at the lateral wall in processes coordinated by the cytoskeletal proteins FtsZ and MreB, respectively.
29443967	10	29	theme	treadmilling	1944:1955	arg1	independent					1924:1934	independent	1924:1934	independent	1924:1934	MurJ recruitment corresponds to a turning point in cytokinesis, which is slow and dependent on FtsZ treadmilling before MurJ arrival but becomes faster and independent of FtsZ treadmilling after peptidoglycan synthesis activity is directed to the septum, where it provides additional force for cell envelope constriction.
29443967	6	30	theme	discrete	1082:1089	arg1	patches					1091:1097	discrete patches	1082:1097	discrete patches that move circumferentially around the cell in tracks perpendicular to the long axis of the cell, and organize the insertion of new cell wall during elongation	1082:1257	The actin homologue MreB forms discrete patches that move circumferentially around the cell in tracks perpendicular to the long axis of the cell, and organize the insertion of new cell wall during elongation.
29443967	0	31	theme	Peptidoglycan	0:12	arg1	synthesis					14:22	Peptidoglycan synthesis	0:22	Peptidoglycan synthesis	0:22	Peptidoglycan synthesis drives an FtsZ-treadmilling-independent step of cytokinesis.
29443967	10	32	theme	MurJ	1888:1891	arg1	arrival					1893:1899	MurJ arrival	1888:1899	MurJ arrival	1888:1899	MurJ recruitment corresponds to a turning point in cytokinesis, which is slow and dependent on FtsZ treadmilling before MurJ arrival but becomes faster and independent of FtsZ treadmilling after peptidoglycan synthesis activity is directed to the septum, where it provides additional force for cell envelope constriction.
29443967	3	33	theme	synthesis	595:603	arg1	machineries					605:615	two peptidoglycan synthesis machineries	577:615	two peptidoglycan synthesis machineries that act at the septum and at the lateral wall in processes coordinated by the cytoskeletal proteins FtsZ and MreB, respectively	577:744	The form of rod-shaped bacteria, such as Bacillus subtilis or Escherichia coli, is generated by the action of two peptidoglycan synthesis machineries that act at the septum and at the lateral wall in processes coordinated by the cytoskeletal proteins FtsZ and MreB, respectively.
29443967	7	34	contain	possess	1296:1302	arg1	Cocci					1260:1264	Cocci	1260:1264	Cocci such as Staphylococcus aureus	1260:1294	Cocci such as Staphylococcus aureus possess only one type of peptidoglycan synthesis machinery, which is diverted from the cell periphery to the septum in preparation for division.
29443967	7	34	contain	possess	1296:1302	arg2	type					1313:1316	only one type	1304:1316	only one type	1304:1316	Cocci such as Staphylococcus aureus possess only one type of peptidoglycan synthesis machinery, which is diverted from the cell periphery to the septum in preparation for division.
29443967	7	34	contain	possess	1296:1302	arg1	aureus					1289:1294	Staphylococcus aureus	1274:1294	Staphylococcus aureus	1274:1294	Cocci such as Staphylococcus aureus possess only one type of peptidoglycan synthesis machinery, which is diverted from the cell periphery to the septum in preparation for division.
29443967	3	35	dep	proteins	709:716	arg1	FtsZ					718:721	FtsZ	718:721	FtsZ	718:721	The form of rod-shaped bacteria, such as Bacillus subtilis or Escherichia coli, is generated by the action of two peptidoglycan synthesis machineries that act at the septum and at the lateral wall in processes coordinated by the cytoskeletal proteins FtsZ and MreB, respectively.
29443967	3	35	dep	proteins	709:716	arg1	proteins					709:716	the cytoskeletal proteins	692:716	the cytoskeletal proteins FtsZ and MreB	692:730	The form of rod-shaped bacteria, such as Bacillus subtilis or Escherichia coli, is generated by the action of two peptidoglycan synthesis machineries that act at the septum and at the lateral wall in processes coordinated by the cytoskeletal proteins FtsZ and MreB, respectively.
29443967	3	35	dep	proteins	709:716	arg1	MreB					727:730	MreB	727:730	MreB	727:730	The form of rod-shaped bacteria, such as Bacillus subtilis or Escherichia coli, is generated by the action of two peptidoglycan synthesis machineries that act at the septum and at the lateral wall in processes coordinated by the cytoskeletal proteins FtsZ and MreB, respectively.
29443967	9	36	theme	DivIB-DivIC-FtsL	1691:1706	arg1	complex					1708:1714	the DivIB-DivIC-FtsL complex	1687:1714	the DivIB-DivIC-FtsL complex	1687:1714	Here we investigate the localization of S. aureus peptidoglycan biosynthesis proteins and show that the recruitment of the putative lipid II flippase MurJ to the septum, by the DivIB-DivIC-FtsL complex, drives peptidoglycan incorporation to the midcell.
29443967	2	37	from	similar	269:275	arg1	bacteria					284:291	all bacteria	280:291	all bacteria	280:291	Peptidoglycan biosynthesis is very similar in all bacteria; bacterial shapes are therefore mainly determined by the spatial and temporal regulation of peptidoglycan synthesis rather than by the chemical composition of peptidoglycan.
29443967	2	38	theme	peptidoglycan	452:464	arg1	composition					437:447	the chemical composition	424:447	the chemical composition of peptidoglycan	424:464	Peptidoglycan biosynthesis is very similar in all bacteria; bacterial shapes are therefore mainly determined by the spatial and temporal regulation of peptidoglycan synthesis rather than by the chemical composition of peptidoglycan.
29443967	5	39	theme	treadmilling	953:964	arg1	rate					945:948	The rate	941:948	The rate of treadmilling in B. subtilis	941:979	The rate of treadmilling in B. subtilis controls the rates of both peptidoglycan synthesis and cell division.
29443967	10	40	theme	synthesis	1977:1985	arg1	activity					1987:1994	peptidoglycan synthesis activity	1963:1994	peptidoglycan synthesis activity	1963:1994	MurJ recruitment corresponds to a turning point in cytokinesis, which is slow and dependent on FtsZ treadmilling before MurJ arrival but becomes faster and independent of FtsZ treadmilling after peptidoglycan synthesis activity is directed to the septum, where it provides additional force for cell envelope constriction.
29443967	9	41	theme	aureus	1557:1562	arg1	proteins					1591:1598	S. aureus peptidoglycan biosynthesis proteins	1554:1598	S. aureus peptidoglycan biosynthesis proteins	1554:1598	Here we investigate the localization of S. aureus peptidoglycan biosynthesis proteins and show that the recruitment of the putative lipid II flippase MurJ to the septum, by the DivIB-DivIC-FtsL complex, drives peptidoglycan incorporation to the midcell.
29443967	8	42	theme	molecular	1445:1453	arg1	cue					1455:1457	The molecular cue	1441:1457	The molecular cue that coordinates this transition	1441:1490	The molecular cue that coordinates this transition has remained elusive.
29443967	4	43	theme	homologue	759:767	arg1	FtsZ					769:772	The tubulin homologue FtsZ	747:772	The tubulin homologue FtsZ	747:772	The tubulin homologue FtsZ is the first protein recruited to the division site, where it assembles in filaments-forming the Z ring-that undergo treadmilling and recruit later divisome proteins.
29443967	4	43	theme	homologue	759:767	arg1	protein					787:793	the first protein	777:793	the first protein recruited to the division site, where it assembles in filaments-forming the Z ring-that undergo treadmilling and recruit later divisome proteins	777:938	The tubulin homologue FtsZ is the first protein recruited to the division site, where it assembles in filaments-forming the Z ring-that undergo treadmilling and recruit later divisome proteins.
29443967	2	44	from	bacteria	284:291	arg1	similar					269:275	similar	269:275	similar	269:275	Peptidoglycan biosynthesis is very similar in all bacteria; bacterial shapes are therefore mainly determined by the spatial and temporal regulation of peptidoglycan synthesis rather than by the chemical composition of peptidoglycan.
29443967	1	45	theme	intracellular	212:224	arg1	turgor					226:231	high intracellular turgor	207:231	high intracellular turgor	207:231	Peptidoglycan is the main component of the bacterial wall and protects cells from the mechanical stress that results from high intracellular turgor.
29443967	9	46	theme	biosynthesis	1578:1589	arg1	proteins					1591:1598	S. aureus peptidoglycan biosynthesis proteins	1554:1598	S. aureus peptidoglycan biosynthesis proteins	1554:1598	Here we investigate the localization of S. aureus peptidoglycan biosynthesis proteins and show that the recruitment of the putative lipid II flippase MurJ to the septum, by the DivIB-DivIC-FtsL complex, drives peptidoglycan incorporation to the midcell.
29443967	6	47	theme	new	1227:1229	arg1	wall					1236:1239	new cell wall	1227:1239	new cell wall	1227:1239	The actin homologue MreB forms discrete patches that move circumferentially around the cell in tracks perpendicular to the long axis of the cell, and organize the insertion of new cell wall during elongation.
29443967	6	48	theme	actin	1055:1059	arg1	MreB					1071:1074	The actin homologue MreB	1051:1074	The actin homologue MreB	1051:1074	The actin homologue MreB forms discrete patches that move circumferentially around the cell in tracks perpendicular to the long axis of the cell, and organize the insertion of new cell wall during elongation.
29443967	6	49	theme	wall	1236:1239	arg1	insertion					1214:1222	the insertion	1210:1222	the insertion of new cell wall	1210:1239	The actin homologue MreB forms discrete patches that move circumferentially around the cell in tracks perpendicular to the long axis of the cell, and organize the insertion of new cell wall during elongation.
29443967	7	50	theme	peptidoglycan	1321:1333	arg1	machinery					1345:1353	peptidoglycan synthesis machinery	1321:1353	peptidoglycan synthesis machinery	1321:1353	Cocci such as Staphylococcus aureus possess only one type of peptidoglycan synthesis machinery, which is diverted from the cell periphery to the septum in preparation for division.
29443967	3	51	theme	rod-shaped	479:488	arg1	subtilis					517:524	Bacillus subtilis	508:524	Bacillus subtilis	508:524	The form of rod-shaped bacteria, such as Bacillus subtilis or Escherichia coli, is generated by the action of two peptidoglycan synthesis machineries that act at the septum and at the lateral wall in processes coordinated by the cytoskeletal proteins FtsZ and MreB, respectively.
29443967	3	51	theme	rod-shaped	479:488	arg1	bacteria					490:497	rod-shaped bacteria	479:497	rod-shaped bacteria	479:497	The form of rod-shaped bacteria, such as Bacillus subtilis or Escherichia coli, is generated by the action of two peptidoglycan synthesis machineries that act at the septum and at the lateral wall in processes coordinated by the cytoskeletal proteins FtsZ and MreB, respectively.
29443967	3	51	theme	rod-shaped	479:488	arg1	coli					541:544	Escherichia coli	529:544	Escherichia coli	529:544	The form of rod-shaped bacteria, such as Bacillus subtilis or Escherichia coli, is generated by the action of two peptidoglycan synthesis machineries that act at the septum and at the lateral wall in processes coordinated by the cytoskeletal proteins FtsZ and MreB, respectively.
29443967	0	52	theme	FtsZ-treadmilling-independent	34:62	arg1	step					64:67	an FtsZ-treadmilling-independent step	31:67	an FtsZ-treadmilling-independent step of cytokinesis	31:82	Peptidoglycan synthesis drives an FtsZ-treadmilling-independent step of cytokinesis.
29443967	7	53	theme	synthesis	1335:1343	arg1	machinery					1345:1353	peptidoglycan synthesis machinery	1321:1353	peptidoglycan synthesis machinery	1321:1353	Cocci such as Staphylococcus aureus possess only one type of peptidoglycan synthesis machinery, which is diverted from the cell periphery to the septum in preparation for division.
29443967	5	54	theme	cell	1036:1039	arg1	division					1041:1048	cell division	1036:1048	cell division	1036:1048	The rate of treadmilling in B. subtilis controls the rates of both peptidoglycan synthesis and cell division.
29443967	9	55	theme	lipid	1646:1650	arg1	MurJ					1664:1667	the putative lipid II flippase MurJ	1633:1667	the putative lipid II flippase MurJ	1633:1667	Here we investigate the localization of S. aureus peptidoglycan biosynthesis proteins and show that the recruitment of the putative lipid II flippase MurJ to the septum, by the DivIB-DivIC-FtsL complex, drives peptidoglycan incorporation to the midcell.
29443967	2	56	theme	chemical	428:435	arg1	composition					437:447	the chemical composition	424:447	the chemical composition of peptidoglycan	424:464	Peptidoglycan biosynthesis is very similar in all bacteria; bacterial shapes are therefore mainly determined by the spatial and temporal regulation of peptidoglycan synthesis rather than by the chemical composition of peptidoglycan.
29443967	0	57	theme	cytokinesis	72:82	arg1	step					64:67	an FtsZ-treadmilling-independent step	31:67	an FtsZ-treadmilling-independent step of cytokinesis	31:82	Peptidoglycan synthesis drives an FtsZ-treadmilling-independent step of cytokinesis.
29443967	9	58	theme	peptidoglycan	1724:1736	arg1	incorporation					1738:1750	peptidoglycan incorporation	1724:1750	peptidoglycan incorporation	1724:1750	Here we investigate the localization of S. aureus peptidoglycan biosynthesis proteins and show that the recruitment of the putative lipid II flippase MurJ to the septum, by the DivIB-DivIC-FtsL complex, drives peptidoglycan incorporation to the midcell.
29443967	3	59	theme	lateral	651:657	arg1	wall					659:662	the lateral wall	647:662	the lateral wall in processes coordinated by the cytoskeletal proteins FtsZ and MreB, respectively	647:744	The form of rod-shaped bacteria, such as Bacillus subtilis or Escherichia coli, is generated by the action of two peptidoglycan synthesis machineries that act at the septum and at the lateral wall in processes coordinated by the cytoskeletal proteins FtsZ and MreB, respectively.
29443967	3	60	theme	machineries	605:615	arg1	action					567:572	the action	563:572	the action of two peptidoglycan synthesis machineries that act at the septum and at the lateral wall in processes coordinated by the cytoskeletal proteins FtsZ and MreB, respectively	563:744	The form of rod-shaped bacteria, such as Bacillus subtilis or Escherichia coli, is generated by the action of two peptidoglycan synthesis machineries that act at the septum and at the lateral wall in processes coordinated by the cytoskeletal proteins FtsZ and MreB, respectively.
29443967	6	61	from	cell	1138:1141	arg1	tracks					1146:1151	tracks	1146:1151	tracks perpendicular to the long axis of the cell	1146:1194	The actin homologue MreB forms discrete patches that move circumferentially around the cell in tracks perpendicular to the long axis of the cell, and organize the insertion of new cell wall during elongation.
29443967	2	62	theme	temporal	362:369	arg1	regulation					371:380	the spatial and temporal regulation	346:380	the spatial and temporal regulation of peptidoglycan synthesis	346:407	Peptidoglycan biosynthesis is very similar in all bacteria; bacterial shapes are therefore mainly determined by the spatial and temporal regulation of peptidoglycan synthesis rather than by the chemical composition of peptidoglycan.
29443967	10	63	theme	FtsZ	1863:1866	arg1	treadmilling					1868:1879	FtsZ treadmilling	1863:1879	FtsZ treadmilling before MurJ arrival	1863:1899	MurJ recruitment corresponds to a turning point in cytokinesis, which is slow and dependent on FtsZ treadmilling before MurJ arrival but becomes faster and independent of FtsZ treadmilling after peptidoglycan synthesis activity is directed to the septum, where it provides additional force for cell envelope constriction.
29443967	2	64	theme	Peptidoglycan	234:246	arg1	biosynthesis					248:259	Peptidoglycan biosynthesis	234:259	Peptidoglycan biosynthesis	234:259	Peptidoglycan biosynthesis is very similar in all bacteria; bacterial shapes are therefore mainly determined by the spatial and temporal regulation of peptidoglycan synthesis rather than by the chemical composition of peptidoglycan.
29443967	1	65	theme	wall	138:141	arg1	component					111:119	the main component	102:119	the main component of the bacterial wall	102:141	Peptidoglycan is the main component of the bacterial wall and protects cells from the mechanical stress that results from high intracellular turgor.
29443967	1	65	theme	wall	138:141	arg1	Peptidoglycan					85:97	Peptidoglycan	85:97	Peptidoglycan	85:97	Peptidoglycan is the main component of the bacterial wall and protects cells from the mechanical stress that results from high intracellular turgor.
29443967	4	66	theme	divisome	922:929	arg1	proteins					931:938	later divisome proteins	916:938	later divisome proteins	916:938	The tubulin homologue FtsZ is the first protein recruited to the division site, where it assembles in filaments-forming the Z ring-that undergo treadmilling and recruit later divisome proteins.
29443967	6	67	theme	cell	1231:1234	arg1	wall					1236:1239	new cell wall	1227:1239	new cell wall	1227:1239	The actin homologue MreB forms discrete patches that move circumferentially around the cell in tracks perpendicular to the long axis of the cell, and organize the insertion of new cell wall during elongation.
29443967	2	68	theme	spatial	350:356	arg1	regulation					371:380	the spatial and temporal regulation	346:380	the spatial and temporal regulation of peptidoglycan synthesis	346:407	Peptidoglycan biosynthesis is very similar in all bacteria; bacterial shapes are therefore mainly determined by the spatial and temporal regulation of peptidoglycan synthesis rather than by the chemical composition of peptidoglycan.
29443967	4	69	theme	later	916:920	arg1	proteins					931:938	later divisome proteins	916:938	later divisome proteins	916:938	The tubulin homologue FtsZ is the first protein recruited to the division site, where it assembles in filaments-forming the Z ring-that undergo treadmilling and recruit later divisome proteins.
29443967	9	70	theme	peptidoglycan	1564:1576	arg1	proteins					1591:1598	S. aureus peptidoglycan biosynthesis proteins	1554:1598	S. aureus peptidoglycan biosynthesis proteins	1554:1598	Here we investigate the localization of S. aureus peptidoglycan biosynthesis proteins and show that the recruitment of the putative lipid II flippase MurJ to the septum, by the DivIB-DivIC-FtsL complex, drives peptidoglycan incorporation to the midcell.
29443967	9	71	theme	putative	1637:1644	arg1	MurJ					1664:1667	the putative lipid II flippase MurJ	1633:1667	the putative lipid II flippase MurJ	1633:1667	Here we investigate the localization of S. aureus peptidoglycan biosynthesis proteins and show that the recruitment of the putative lipid II flippase MurJ to the septum, by the DivIB-DivIC-FtsL complex, drives peptidoglycan incorporation to the midcell.
29443967	6	72	theme	perpendicular	1153:1165	arg1	tracks					1146:1151	tracks	1146:1151	tracks perpendicular to the long axis of the cell	1146:1194	The actin homologue MreB forms discrete patches that move circumferentially around the cell in tracks perpendicular to the long axis of the cell, and organize the insertion of new cell wall during elongation.
29443967	10	73	from	point	1810:1814	arg1	cytokinesis					1819:1829	cytokinesis	1819:1829	cytokinesis	1819:1829	MurJ recruitment corresponds to a turning point in cytokinesis, which is slow and dependent on FtsZ treadmilling before MurJ arrival but becomes faster and independent of FtsZ treadmilling after peptidoglycan synthesis activity is directed to the septum, where it provides additional force for cell envelope constriction.
29443967	3	74	theme	bacteria	490:497	arg1	form					471:474	The form	467:474	The form of rod-shaped bacteria, such as Bacillus subtilis or Escherichia coli,	467:545	The form of rod-shaped bacteria, such as Bacillus subtilis or Escherichia coli, is generated by the action of two peptidoglycan synthesis machineries that act at the septum and at the lateral wall in processes coordinated by the cytoskeletal proteins FtsZ and MreB, respectively.
29443967	4	75	theme	division	812:819	arg1	site					821:824	the division site	808:824	the division site	808:824	The tubulin homologue FtsZ is the first protein recruited to the division site, where it assembles in filaments-forming the Z ring-that undergo treadmilling and recruit later divisome proteins.
29443967	1	76	theme	high	207:210	arg1	turgor					226:231	high intracellular turgor	207:231	high intracellular turgor	207:231	Peptidoglycan is the main component of the bacterial wall and protects cells from the mechanical stress that results from high intracellular turgor.
29443967	2	77	theme	synthesis	399:407	arg1	regulation					371:380	the spatial and temporal regulation	346:380	the spatial and temporal regulation of peptidoglycan synthesis	346:407	Peptidoglycan biosynthesis is very similar in all bacteria; bacterial shapes are therefore mainly determined by the spatial and temporal regulation of peptidoglycan synthesis rather than by the chemical composition of peptidoglycan.
29443967	10	78	theme	envelope	2067:2074	arg1	constriction					2076:2087	cell envelope constriction	2062:2087	cell envelope constriction	2062:2087	MurJ recruitment corresponds to a turning point in cytokinesis, which is slow and dependent on FtsZ treadmilling before MurJ arrival but becomes faster and independent of FtsZ treadmilling after peptidoglycan synthesis activity is directed to the septum, where it provides additional force for cell envelope constriction.
29443967	4	79	dep	ring-that	873:881	arg1	undergo					883:889	undergo	883:889	undergo treadmilling	883:902	The tubulin homologue FtsZ is the first protein recruited to the division site, where it assembles in filaments-forming the Z ring-that undergo treadmilling and recruit later divisome proteins.
29443967	4	79	dep	ring-that	873:881	arg1	recruit					908:914	recruit	908:914	recruit later divisome proteins	908:938	The tubulin homologue FtsZ is the first protein recruited to the division site, where it assembles in filaments-forming the Z ring-that undergo treadmilling and recruit later divisome proteins.
29443967	5	80	theme	synthesis	1022:1030	arg1	rates					994:998	the rates	990:998	the rates of both peptidoglycan synthesis and cell division	990:1048	The rate of treadmilling in B. subtilis controls the rates of both peptidoglycan synthesis and cell division.
29443967	1	81	from	stress	182:187	arg1	cells					156:160	cells	156:160	cells from the mechanical stress that results from high intracellular turgor	156:231	Peptidoglycan is the main component of the bacterial wall and protects cells from the mechanical stress that results from high intracellular turgor.
29443967	2	82	theme	peptidoglycan	385:397	arg1	synthesis					399:407	peptidoglycan synthesis	385:407	peptidoglycan synthesis	385:407	Peptidoglycan biosynthesis is very similar in all bacteria; bacterial shapes are therefore mainly determined by the spatial and temporal regulation of peptidoglycan synthesis rather than by the chemical composition of peptidoglycan.
29381472	2	0	theme	extracts	637:644	arg1	activity					616:623	the antioxidant activity	600:623	the antioxidant activity of phenolic extracts from I. gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark	600:701	This work characterized the phenolic composition, evaluated the cholinergic enzymes (acetylcholinesterase, AChE and butyrylcholinesterase, BChE) inhibition, and assessed the antioxidant activity of phenolic extracts from I. gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark.
29381472	6	1	theme	DPPH	1198:1201	arg1	ability					1222:1228	DPPH radical scavenging ability	1198:1228	DPPH radical scavenging ability (IC50=19.98 μg/mL)	1198:1247	The phenolic extracts displayed DPPH radical scavenging ability (IC50=19.98 μg/mL), ABTS radical scavenging ability (IC50=18.25 μg/mL), iron chelation (IC50=113.10 μg/mL), and reducing power (Fe3+ to Fe2+) (5.94 mg ascorbic acid equivalent/100 g).
29381472	6	1	theme	DPPH	1198:1201	arg1	μg/mL					1242:1246	IC50=19.98 μg/mL	1231:1246	IC50=19.98 μg/mL	1231:1246	The phenolic extracts displayed DPPH radical scavenging ability (IC50=19.98 μg/mL), ABTS radical scavenging ability (IC50=18.25 μg/mL), iron chelation (IC50=113.10 μg/mL), and reducing power (Fe3+ to Fe2+) (5.94 mg ascorbic acid equivalent/100 g).
29381472	1	2	theme	medicinal	280:288	arg1	plant					290:294	a medicinal plant	278:294	a medicinal plant used in most parts of Africa to manage a number of ailments including neurodegenerative diseases that occur without scientific basis	278:427	BACKGROUND Irvingia gabonensis stem bark is a medicinal plant used in most parts of Africa to manage a number of ailments including neurodegenerative diseases that occur without scientific basis.
29381472	1	2	theme	medicinal	280:288	arg1	bark					270:273	BACKGROUND Irvingia gabonensis stem bark	234:273	BACKGROUND Irvingia gabonensis stem bark	234:273	BACKGROUND Irvingia gabonensis stem bark is a medicinal plant used in most parts of Africa to manage a number of ailments including neurodegenerative diseases that occur without scientific basis.
29381472	7	3	theme	I.	1426:1427	arg1	gabonensis					1429:1438	I. gabonensis	1426:1438	I. gabonensis	1426:1438	Extracts of I. gabonensis inhibited AChE (IC50=32.90 μg/mL) and BChE (IC50=41.50 μg/mL) activities in concentration-dependent manner.
29381472	3	4	theme	flavonoids	729:738	arg1	content					740:746	flavonoids content	729:746	flavonoids content	729:746	METHODS Total phenol and flavonoids content was evaluated in addition to antioxidant activity as shown by Fe2+ chelation, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability, and 2,2-azino-bis-(3-ethylbenthiazoline-6-sulfonic acid) (ABTS) radical scavenging ability.
29381472	3	4	theme	flavonoids	729:738	arg1	METHODS					704:710	METHODS	704:710	METHODS Total phenol and flavonoids content	704:746	METHODS Total phenol and flavonoids content was evaluated in addition to antioxidant activity as shown by Fe2+ chelation, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability, and 2,2-azino-bis-(3-ethylbenthiazoline-6-sulfonic acid) (ABTS) radical scavenging ability.
29381472	6	5	theme	scavenging	1263:1272	arg1	ability					1274:1280	ABTS radical scavenging ability	1250:1280	ABTS radical scavenging ability (IC50=18.25 μg/mL)	1250:1299	The phenolic extracts displayed DPPH radical scavenging ability (IC50=19.98 μg/mL), ABTS radical scavenging ability (IC50=18.25 μg/mL), iron chelation (IC50=113.10 μg/mL), and reducing power (Fe3+ to Fe2+) (5.94 mg ascorbic acid equivalent/100 g).
29381472	6	5	theme	scavenging	1263:1272	arg1	μg/mL					1294:1298	IC50=18.25 μg/mL	1283:1298	IC50=18.25 μg/mL	1283:1298	The phenolic extracts displayed DPPH radical scavenging ability (IC50=19.98 μg/mL), ABTS radical scavenging ability (IC50=18.25 μg/mL), iron chelation (IC50=113.10 μg/mL), and reducing power (Fe3+ to Fe2+) (5.94 mg ascorbic acid equivalent/100 g).
29381472	2	6	dep	I.	651:652	arg1	Aubry-Lecomte					666:678	Aubry-Lecomte	666:678	Aubry-Lecomte	666:678	This work characterized the phenolic composition, evaluated the cholinergic enzymes (acetylcholinesterase, AChE and butyrylcholinesterase, BChE) inhibition, and assessed the antioxidant activity of phenolic extracts from I. gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark.
29381472	2	6	dep	I.	651:652	arg1	gabonensis					654:663	I. gabonensis	651:663	I. gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark	651:701	This work characterized the phenolic composition, evaluated the cholinergic enzymes (acetylcholinesterase, AChE and butyrylcholinesterase, BChE) inhibition, and assessed the antioxidant activity of phenolic extracts from I. gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark.
29381472	2	7	theme	I.	651:652	arg1	bark					698:701	I. gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark	651:701	I. gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark	651:701	This work characterized the phenolic composition, evaluated the cholinergic enzymes (acetylcholinesterase, AChE and butyrylcholinesterase, BChE) inhibition, and assessed the antioxidant activity of phenolic extracts from I. gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark.
29381472	6	8	theme	ABTS	1250:1253	arg1	ability					1274:1280	ABTS radical scavenging ability	1250:1280	ABTS radical scavenging ability (IC50=18.25 μg/mL)	1250:1299	The phenolic extracts displayed DPPH radical scavenging ability (IC50=19.98 μg/mL), ABTS radical scavenging ability (IC50=18.25 μg/mL), iron chelation (IC50=113.10 μg/mL), and reducing power (Fe3+ to Fe2+) (5.94 mg ascorbic acid equivalent/100 g).
29381472	6	8	theme	ABTS	1250:1253	arg1	μg/mL					1294:1298	IC50=18.25 μg/mL	1283:1298	IC50=18.25 μg/mL	1283:1298	The phenolic extracts displayed DPPH radical scavenging ability (IC50=19.98 μg/mL), ABTS radical scavenging ability (IC50=18.25 μg/mL), iron chelation (IC50=113.10 μg/mL), and reducing power (Fe3+ to Fe2+) (5.94 mg ascorbic acid equivalent/100 g).
29381472	0	9	theme	phenolic	152:159	arg1	extracts					161:168	phenolic extracts	152:168	phenolic extracts from Irvingia gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark	152:231	Chromatographic fingerprint analysis, antioxidant properties, and inhibition of cholinergic enzymes (acetylcholinesterase and butyrylcholinesterase) of phenolic extracts from Irvingia gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark.
29381472	0	10	dep	Aubry-Lecomte	196:208	arg1	Rorke					215:219	Rorke	215:219	Rorke	215:219	Chromatographic fingerprint analysis, antioxidant properties, and inhibition of cholinergic enzymes (acetylcholinesterase and butyrylcholinesterase) of phenolic extracts from Irvingia gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark.
29381472	0	10	dep	Aubry-Lecomte	196:208	arg1	ex					210:211	Aubry-Lecomte ex	196:211	Aubry-Lecomte ex O'Rorke	196:219	Chromatographic fingerprint analysis, antioxidant properties, and inhibition of cholinergic enzymes (acetylcholinesterase and butyrylcholinesterase) of phenolic extracts from Irvingia gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark.
29381472	2	11	theme	antioxidant	604:614	arg1	activity					616:623	the antioxidant activity	600:623	the antioxidant activity of phenolic extracts from I. gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark	600:701	This work characterized the phenolic composition, evaluated the cholinergic enzymes (acetylcholinesterase, AChE and butyrylcholinesterase, BChE) inhibition, and assessed the antioxidant activity of phenolic extracts from I. gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark.
29381472	0	12	from	inhibition	66:75	arg1	bark					228:231	Irvingia gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark	175:231	Irvingia gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark	175:231	Chromatographic fingerprint analysis, antioxidant properties, and inhibition of cholinergic enzymes (acetylcholinesterase and butyrylcholinesterase) of phenolic extracts from Irvingia gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark.
29381472	0	13	dep	gabonensis	184:193	arg1	Aubry-Lecomte					196:208	Aubry-Lecomte	196:208	Aubry-Lecomte	196:208	Chromatographic fingerprint analysis, antioxidant properties, and inhibition of cholinergic enzymes (acetylcholinesterase and butyrylcholinesterase) of phenolic extracts from Irvingia gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark.
29381472	0	14	theme	gabonensis	184:193	arg1	bark					228:231	Irvingia gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark	175:231	Irvingia gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark	175:231	Chromatographic fingerprint analysis, antioxidant properties, and inhibition of cholinergic enzymes (acetylcholinesterase and butyrylcholinesterase) of phenolic extracts from Irvingia gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark.
29381472	5	15	theme	phenolic	1082:1089	arg1	acid					1091:1094	phenolic acid	1082:1094	phenolic acid (ellagic acid)	1082:1109	RESULTS The extract was found to be rich in phenolic acid (ellagic acid) and flavonoids (quercetrin, kaempferol, and apigenin).
29381472	5	15	theme	phenolic	1082:1089	arg1	acid					1105:1108	ellagic acid	1097:1108	ellagic acid	1097:1108	RESULTS The extract was found to be rich in phenolic acid (ellagic acid) and flavonoids (quercetrin, kaempferol, and apigenin).
29381472	3	16	theme	scavenging	871:880	arg1	ability					882:888	2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability	826:888	2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability	826:888	METHODS Total phenol and flavonoids content was evaluated in addition to antioxidant activity as shown by Fe2+ chelation, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability, and 2,2-azino-bis-(3-ethylbenthiazoline-6-sulfonic acid) (ABTS) radical scavenging ability.
29381472	6	17	theme	scavenging	1211:1220	arg1	ability					1222:1228	DPPH radical scavenging ability	1198:1228	DPPH radical scavenging ability (IC50=19.98 μg/mL)	1198:1247	The phenolic extracts displayed DPPH radical scavenging ability (IC50=19.98 μg/mL), ABTS radical scavenging ability (IC50=18.25 μg/mL), iron chelation (IC50=113.10 μg/mL), and reducing power (Fe3+ to Fe2+) (5.94 mg ascorbic acid equivalent/100 g).
29381472	6	17	theme	scavenging	1211:1220	arg1	μg/mL					1242:1246	IC50=19.98 μg/mL	1231:1246	IC50=19.98 μg/mL	1231:1246	The phenolic extracts displayed DPPH radical scavenging ability (IC50=19.98 μg/mL), ABTS radical scavenging ability (IC50=18.25 μg/mL), iron chelation (IC50=113.10 μg/mL), and reducing power (Fe3+ to Fe2+) (5.94 mg ascorbic acid equivalent/100 g).
29381472	3	18	theme	Fe2+	810:813	arg1	chelation					815:823	Fe2+ chelation	810:823	Fe2+ chelation	810:823	METHODS Total phenol and flavonoids content was evaluated in addition to antioxidant activity as shown by Fe2+ chelation, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability, and 2,2-azino-bis-(3-ethylbenthiazoline-6-sulfonic acid) (ABTS) radical scavenging ability.
29381472	5	19	from	acid	1091:1094	arg1	rich					1074:1077	rich	1074:1077	rich	1074:1077	RESULTS The extract was found to be rich in phenolic acid (ellagic acid) and flavonoids (quercetrin, kaempferol, and apigenin).
29381472	5	19	from	acid	1091:1094	arg1	extract					1050:1056	The extract	1046:1056	The extract	1046:1056	RESULTS The extract was found to be rich in phenolic acid (ellagic acid) and flavonoids (quercetrin, kaempferol, and apigenin).
29381472	6	20	theme	acid	1390:1393	arg1	power					1351:1355	reducing power	1342:1355	reducing power (Fe3+ to Fe2+) (5.94 mg ascorbic acid equivalent/100 g)	1342:1411	The phenolic extracts displayed DPPH radical scavenging ability (IC50=19.98 μg/mL), ABTS radical scavenging ability (IC50=18.25 μg/mL), iron chelation (IC50=113.10 μg/mL), and reducing power (Fe3+ to Fe2+) (5.94 mg ascorbic acid equivalent/100 g).
29381472	6	20	theme	acid	1390:1393	arg1	g					1410:1410	5.94 mg ascorbic acid equivalent/100 g	1373:1410	5.94 mg ascorbic acid equivalent/100 g	1373:1410	The phenolic extracts displayed DPPH radical scavenging ability (IC50=19.98 μg/mL), ABTS radical scavenging ability (IC50=18.25 μg/mL), iron chelation (IC50=113.10 μg/mL), and reducing power (Fe3+ to Fe2+) (5.94 mg ascorbic acid equivalent/100 g).
29381472	3	21	theme	3-ethylbenthiazoline-6-sulfonic	910:940	arg1	acid					942:945	3-ethylbenthiazoline-6-sulfonic acid	910:945	3-ethylbenthiazoline-6-sulfonic acid	910:945	METHODS Total phenol and flavonoids content was evaluated in addition to antioxidant activity as shown by Fe2+ chelation, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability, and 2,2-azino-bis-(3-ethylbenthiazoline-6-sulfonic acid) (ABTS) radical scavenging ability.
29381472	0	22	from	analysis	28:35	arg1	bark					228:231	Irvingia gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark	175:231	Irvingia gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark	175:231	Chromatographic fingerprint analysis, antioxidant properties, and inhibition of cholinergic enzymes (acetylcholinesterase and butyrylcholinesterase) of phenolic extracts from Irvingia gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark.
29381472	0	23	theme	cholinergic	80:90	arg1	enzymes					92:98	cholinergic enzymes	80:98	cholinergic enzymes	80:98	Chromatographic fingerprint analysis, antioxidant properties, and inhibition of cholinergic enzymes (acetylcholinesterase and butyrylcholinesterase) of phenolic extracts from Irvingia gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark.
29381472	3	24	theme	2,2-azino-bis-	895:908	arg1	ability					974:980	2,2-azino-bis-(3-ethylbenthiazoline-6-sulfonic acid) (ABTS) radical scavenging ability	895:980	2,2-azino-bis-(3-ethylbenthiazoline-6-sulfonic acid) (ABTS) radical scavenging ability	895:980	METHODS Total phenol and flavonoids content was evaluated in addition to antioxidant activity as shown by Fe2+ chelation, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability, and 2,2-azino-bis-(3-ethylbenthiazoline-6-sulfonic acid) (ABTS) radical scavenging ability.
29381472	7	25	theme	concentration-dependent	1516:1538	arg1	manner					1540:1545	concentration-dependent manner	1516:1545	concentration-dependent manner	1516:1545	Extracts of I. gabonensis inhibited AChE (IC50=32.90 μg/mL) and BChE (IC50=41.50 μg/mL) activities in concentration-dependent manner.
29381472	1	26	theme	ailments	347:354	arg1	number					337:342	a number	335:342	a number of ailments including neurodegenerative diseases that occur without scientific basis	335:427	BACKGROUND Irvingia gabonensis stem bark is a medicinal plant used in most parts of Africa to manage a number of ailments including neurodegenerative diseases that occur without scientific basis.
29381472	1	26	theme	ailments	347:354	arg1	diseases					384:391	neurodegenerative diseases	366:391	neurodegenerative diseases that occur without scientific basis	366:427	BACKGROUND Irvingia gabonensis stem bark is a medicinal plant used in most parts of Africa to manage a number of ailments including neurodegenerative diseases that occur without scientific basis.
29381472	6	27	dep	power	1351:1355	arg1	Fe3+					1358:1361	Fe3+	1358:1361	Fe3+ to Fe2+	1358:1369	The phenolic extracts displayed DPPH radical scavenging ability (IC50=19.98 μg/mL), ABTS radical scavenging ability (IC50=18.25 μg/mL), iron chelation (IC50=113.10 μg/mL), and reducing power (Fe3+ to Fe2+) (5.94 mg ascorbic acid equivalent/100 g).
29381472	1	28	theme	neurodegenerative	366:382	arg1	diseases					384:391	neurodegenerative diseases	366:391	neurodegenerative diseases that occur without scientific basis	366:427	BACKGROUND Irvingia gabonensis stem bark is a medicinal plant used in most parts of Africa to manage a number of ailments including neurodegenerative diseases that occur without scientific basis.
29381472	3	29	dep	2,2-azino-bis-	895:908	arg1	ABTS					949:952	ABTS	949:952	ABTS	949:952	METHODS Total phenol and flavonoids content was evaluated in addition to antioxidant activity as shown by Fe2+ chelation, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability, and 2,2-azino-bis-(3-ethylbenthiazoline-6-sulfonic acid) (ABTS) radical scavenging ability.
29381472	3	29	dep	2,2-azino-bis-	895:908	arg1	acid					942:945	3-ethylbenthiazoline-6-sulfonic acid	910:945	3-ethylbenthiazoline-6-sulfonic acid	910:945	METHODS Total phenol and flavonoids content was evaluated in addition to antioxidant activity as shown by Fe2+ chelation, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability, and 2,2-azino-bis-(3-ethylbenthiazoline-6-sulfonic acid) (ABTS) radical scavenging ability.
29381472	0	30	theme	Chromatographic	0:14	arg1	analysis					28:35	Chromatographic fingerprint analysis	0:35	Chromatographic fingerprint analysis	0:35	Chromatographic fingerprint analysis, antioxidant properties, and inhibition of cholinergic enzymes (acetylcholinesterase and butyrylcholinesterase) of phenolic extracts from Irvingia gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark.
29381472	3	31	dep	METHODS	704:710	arg1	phenol					718:723	Total phenol	712:723	Total phenol	712:723	METHODS Total phenol and flavonoids content was evaluated in addition to antioxidant activity as shown by Fe2+ chelation, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability, and 2,2-azino-bis-(3-ethylbenthiazoline-6-sulfonic acid) (ABTS) radical scavenging ability.
29381472	3	31	dep	METHODS	704:710	arg1	content					740:746	flavonoids content	729:746	flavonoids content	729:746	METHODS Total phenol and flavonoids content was evaluated in addition to antioxidant activity as shown by Fe2+ chelation, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability, and 2,2-azino-bis-(3-ethylbenthiazoline-6-sulfonic acid) (ABTS) radical scavenging ability.
29381472	3	31	dep	METHODS	704:710	arg1	METHODS					704:710	METHODS	704:710	METHODS Total phenol and flavonoids content	704:746	METHODS Total phenol and flavonoids content was evaluated in addition to antioxidant activity as shown by Fe2+ chelation, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability, and 2,2-azino-bis-(3-ethylbenthiazoline-6-sulfonic acid) (ABTS) radical scavenging ability.
29381472	2	32	from	activity	616:623	arg1	bark					698:701	I. gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark	651:701	I. gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark	651:701	This work characterized the phenolic composition, evaluated the cholinergic enzymes (acetylcholinesterase, AChE and butyrylcholinesterase, BChE) inhibition, and assessed the antioxidant activity of phenolic extracts from I. gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark.
29381472	5	33	from	flavonoids	1115:1124	arg1	rich					1074:1077	rich	1074:1077	rich	1074:1077	RESULTS The extract was found to be rich in phenolic acid (ellagic acid) and flavonoids (quercetrin, kaempferol, and apigenin).
29381472	5	33	from	flavonoids	1115:1124	arg1	extract					1050:1056	The extract	1046:1056	The extract	1046:1056	RESULTS The extract was found to be rich in phenolic acid (ellagic acid) and flavonoids (quercetrin, kaempferol, and apigenin).
29381472	3	34	theme	radical	955:961	arg1	ability					974:980	2,2-azino-bis-(3-ethylbenthiazoline-6-sulfonic acid) (ABTS) radical scavenging ability	895:980	2,2-azino-bis-(3-ethylbenthiazoline-6-sulfonic acid) (ABTS) radical scavenging ability	895:980	METHODS Total phenol and flavonoids content was evaluated in addition to antioxidant activity as shown by Fe2+ chelation, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability, and 2,2-azino-bis-(3-ethylbenthiazoline-6-sulfonic acid) (ABTS) radical scavenging ability.
29381472	2	35	theme	cholinergic	494:504	arg1	acetylcholinesterase					515:534	acetylcholinesterase	515:534	acetylcholinesterase	515:534	This work characterized the phenolic composition, evaluated the cholinergic enzymes (acetylcholinesterase, AChE and butyrylcholinesterase, BChE) inhibition, and assessed the antioxidant activity of phenolic extracts from I. gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark.
29381472	2	35	theme	cholinergic	494:504	arg1	enzymes					506:512	the cholinergic enzymes	490:512	the cholinergic enzymes (acetylcholinesterase, AChE and butyrylcholinesterase, BChE) inhibition	490:584	This work characterized the phenolic composition, evaluated the cholinergic enzymes (acetylcholinesterase, AChE and butyrylcholinesterase, BChE) inhibition, and assessed the antioxidant activity of phenolic extracts from I. gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark.
29381472	2	35	theme	cholinergic	494:504	arg1	butyrylcholinesterase					546:566	butyrylcholinesterase	546:566	butyrylcholinesterase	546:566	This work characterized the phenolic composition, evaluated the cholinergic enzymes (acetylcholinesterase, AChE and butyrylcholinesterase, BChE) inhibition, and assessed the antioxidant activity of phenolic extracts from I. gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark.
29381472	2	35	theme	cholinergic	494:504	arg1	AChE					537:540	AChE	537:540	AChE	537:540	This work characterized the phenolic composition, evaluated the cholinergic enzymes (acetylcholinesterase, AChE and butyrylcholinesterase, BChE) inhibition, and assessed the antioxidant activity of phenolic extracts from I. gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark.
29381472	6	36	theme	mg	1378:1379	arg1	power					1351:1355	reducing power	1342:1355	reducing power (Fe3+ to Fe2+) (5.94 mg ascorbic acid equivalent/100 g)	1342:1411	The phenolic extracts displayed DPPH radical scavenging ability (IC50=19.98 μg/mL), ABTS radical scavenging ability (IC50=18.25 μg/mL), iron chelation (IC50=113.10 μg/mL), and reducing power (Fe3+ to Fe2+) (5.94 mg ascorbic acid equivalent/100 g).
29381472	6	36	theme	mg	1378:1379	arg1	g					1410:1410	5.94 mg ascorbic acid equivalent/100 g	1373:1410	5.94 mg ascorbic acid equivalent/100 g	1373:1410	The phenolic extracts displayed DPPH radical scavenging ability (IC50=19.98 μg/mL), ABTS radical scavenging ability (IC50=18.25 μg/mL), iron chelation (IC50=113.10 μg/mL), and reducing power (Fe3+ to Fe2+) (5.94 mg ascorbic acid equivalent/100 g).
29381472	0	37	theme	antioxidant	38:48	arg1	properties					50:59	antioxidant properties	38:59	antioxidant properties	38:59	Chromatographic fingerprint analysis, antioxidant properties, and inhibition of cholinergic enzymes (acetylcholinesterase and butyrylcholinesterase) of phenolic extracts from Irvingia gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark.
29381472	7	38	theme	BChE	1478:1481	arg1	activities					1502:1511	AChE (IC50=32.90 μg/mL) and BChE (IC50=41.50 μg/mL) activities	1450:1511	activities	1502:1511	Extracts of I. gabonensis inhibited AChE (IC50=32.90 μg/mL) and BChE (IC50=41.50 μg/mL) activities in concentration-dependent manner.
29381472	7	38	theme	BChE	1478:1481	arg1	μg/mL					1495:1499	IC50=41.50 μg/mL	1484:1499	IC50=41.50 μg/mL	1484:1499	Extracts of I. gabonensis inhibited AChE (IC50=32.90 μg/mL) and BChE (IC50=41.50 μg/mL) activities in concentration-dependent manner.
29381472	0	39	from	bark	228:231	arg1	analysis					28:35	Chromatographic fingerprint analysis	0:35	Chromatographic fingerprint analysis	0:35	Chromatographic fingerprint analysis, antioxidant properties, and inhibition of cholinergic enzymes (acetylcholinesterase and butyrylcholinesterase) of phenolic extracts from Irvingia gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark.
29381472	0	39	from	bark	228:231	arg1	properties					50:59	antioxidant properties	38:59	antioxidant properties	38:59	Chromatographic fingerprint analysis, antioxidant properties, and inhibition of cholinergic enzymes (acetylcholinesterase and butyrylcholinesterase) of phenolic extracts from Irvingia gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark.
29381472	0	39	from	bark	228:231	arg1	inhibition					66:75	inhibition	66:75	inhibition of cholinergic enzymes	66:98	Chromatographic fingerprint analysis, antioxidant properties, and inhibition of cholinergic enzymes (acetylcholinesterase and butyrylcholinesterase) of phenolic extracts from Irvingia gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark.
29381472	0	39	from	bark	228:231	arg1	extracts					161:168	phenolic extracts	152:168	phenolic extracts from Irvingia gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark	152:231	Chromatographic fingerprint analysis, antioxidant properties, and inhibition of cholinergic enzymes (acetylcholinesterase and butyrylcholinesterase) of phenolic extracts from Irvingia gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark.
29381472	1	40	theme	Africa	318:323	arg1	parts					309:313	most parts	304:313	most parts of Africa	304:323	BACKGROUND Irvingia gabonensis stem bark is a medicinal plant used in most parts of Africa to manage a number of ailments including neurodegenerative diseases that occur without scientific basis.
29381472	5	41	dep	flavonoids	1115:1124	arg1	kaempferol					1139:1148	kaempferol	1139:1148	kaempferol	1139:1148	RESULTS The extract was found to be rich in phenolic acid (ellagic acid) and flavonoids (quercetrin, kaempferol, and apigenin).
29381472	5	41	dep	flavonoids	1115:1124	arg1	apigenin					1155:1162	apigenin	1155:1162	apigenin	1155:1162	RESULTS The extract was found to be rich in phenolic acid (ellagic acid) and flavonoids (quercetrin, kaempferol, and apigenin).
29381472	5	41	dep	flavonoids	1115:1124	arg1	quercetrin					1127:1136	quercetrin	1127:1136	quercetrin	1127:1136	RESULTS The extract was found to be rich in phenolic acid (ellagic acid) and flavonoids (quercetrin, kaempferol, and apigenin).
29381472	5	41	dep	flavonoids	1115:1124	arg1	flavonoids					1115:1124	flavonoids	1115:1124	flavonoids (quercetrin, kaempferol, and apigenin)	1115:1163	RESULTS The extract was found to be rich in phenolic acid (ellagic acid) and flavonoids (quercetrin, kaempferol, and apigenin).
29381472	1	42	theme	BACKGROUND	234:243	arg1	plant					290:294	a medicinal plant	278:294	a medicinal plant used in most parts of Africa to manage a number of ailments including neurodegenerative diseases that occur without scientific basis	278:427	BACKGROUND Irvingia gabonensis stem bark is a medicinal plant used in most parts of Africa to manage a number of ailments including neurodegenerative diseases that occur without scientific basis.
29381472	1	42	theme	BACKGROUND	234:243	arg1	bark					270:273	BACKGROUND Irvingia gabonensis stem bark	234:273	BACKGROUND Irvingia gabonensis stem bark	234:273	BACKGROUND Irvingia gabonensis stem bark is a medicinal plant used in most parts of Africa to manage a number of ailments including neurodegenerative diseases that occur without scientific basis.
29381472	2	43	dep	acetylcholinesterase	515:534	arg1	BChE					569:572	BChE	569:572	BChE	569:572	This work characterized the phenolic composition, evaluated the cholinergic enzymes (acetylcholinesterase, AChE and butyrylcholinesterase, BChE) inhibition, and assessed the antioxidant activity of phenolic extracts from I. gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark.
29381472	0	44	dep	analysis	28:35	arg1	acetylcholinesterase					101:120	acetylcholinesterase	101:120	acetylcholinesterase	101:120	Chromatographic fingerprint analysis, antioxidant properties, and inhibition of cholinergic enzymes (acetylcholinesterase and butyrylcholinesterase) of phenolic extracts from Irvingia gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark.
29381472	0	44	dep	analysis	28:35	arg1	butyrylcholinesterase					126:146	butyrylcholinesterase	126:146	butyrylcholinesterase	126:146	Chromatographic fingerprint analysis, antioxidant properties, and inhibition of cholinergic enzymes (acetylcholinesterase and butyrylcholinesterase) of phenolic extracts from Irvingia gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark.
29381472	1	45	theme	gabonensis	254:263	arg1	plant					290:294	a medicinal plant	278:294	a medicinal plant used in most parts of Africa to manage a number of ailments including neurodegenerative diseases that occur without scientific basis	278:427	BACKGROUND Irvingia gabonensis stem bark is a medicinal plant used in most parts of Africa to manage a number of ailments including neurodegenerative diseases that occur without scientific basis.
29381472	1	45	theme	gabonensis	254:263	arg1	bark					270:273	BACKGROUND Irvingia gabonensis stem bark	234:273	BACKGROUND Irvingia gabonensis stem bark	234:273	BACKGROUND Irvingia gabonensis stem bark is a medicinal plant used in most parts of Africa to manage a number of ailments including neurodegenerative diseases that occur without scientific basis.
29381472	5	46	from	rich	1074:1077	arg1	kaempferol					1139:1148	kaempferol	1139:1148	kaempferol	1139:1148	RESULTS The extract was found to be rich in phenolic acid (ellagic acid) and flavonoids (quercetrin, kaempferol, and apigenin).
29381472	5	46	from	rich	1074:1077	arg1	acid					1091:1094	phenolic acid	1082:1094	phenolic acid (ellagic acid)	1082:1109	RESULTS The extract was found to be rich in phenolic acid (ellagic acid) and flavonoids (quercetrin, kaempferol, and apigenin).
29381472	5	46	from	rich	1074:1077	arg1	flavonoids					1115:1124	flavonoids	1115:1124	flavonoids (quercetrin, kaempferol, and apigenin)	1115:1163	RESULTS The extract was found to be rich in phenolic acid (ellagic acid) and flavonoids (quercetrin, kaempferol, and apigenin).
29381472	5	46	from	rich	1074:1077	arg1	apigenin					1155:1162	apigenin	1155:1162	apigenin	1155:1162	RESULTS The extract was found to be rich in phenolic acid (ellagic acid) and flavonoids (quercetrin, kaempferol, and apigenin).
29381472	5	46	from	rich	1074:1077	arg1	quercetrin					1127:1136	quercetrin	1127:1136	quercetrin	1127:1136	RESULTS The extract was found to be rich in phenolic acid (ellagic acid) and flavonoids (quercetrin, kaempferol, and apigenin).
29381472	5	46	from	rich	1074:1077	arg1	acid					1105:1108	ellagic acid	1097:1108	ellagic acid	1097:1108	RESULTS The extract was found to be rich in phenolic acid (ellagic acid) and flavonoids (quercetrin, kaempferol, and apigenin).
29381472	8	47	theme	disease	1646:1652	arg1	activity					1654:1661	their anti-Alzheimer's disease activity	1623:1661	their anti-Alzheimer's disease activity	1623:1661	CONCLUSIONS Hence, possible mechanism through which the stem bark executes their anti-Alzheimer's disease activity might be by inhibiting cholinesterase activities in addition to suppressing oxidative-stress-induced neurodegeneration.
29381472	7	48	theme	gabonensis	1429:1438	arg1	Extracts					1414:1421	Extracts	1414:1421	Extracts of I. gabonensis	1414:1438	Extracts of I. gabonensis inhibited AChE (IC50=32.90 μg/mL) and BChE (IC50=41.50 μg/mL) activities in concentration-dependent manner.
29381472	2	49	theme	phenolic	628:635	arg1	extracts					637:644	phenolic extracts	628:644	phenolic extracts from I. gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark	628:701	This work characterized the phenolic composition, evaluated the cholinergic enzymes (acetylcholinesterase, AChE and butyrylcholinesterase, BChE) inhibition, and assessed the antioxidant activity of phenolic extracts from I. gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark.
29381472	3	50	theme	Total	712:716	arg1	phenol					718:723	Total phenol	712:723	Total phenol	712:723	METHODS Total phenol and flavonoids content was evaluated in addition to antioxidant activity as shown by Fe2+ chelation, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability, and 2,2-azino-bis-(3-ethylbenthiazoline-6-sulfonic acid) (ABTS) radical scavenging ability.
29381472	3	50	theme	Total	712:716	arg1	METHODS					704:710	METHODS	704:710	METHODS Total phenol and flavonoids content	704:746	METHODS Total phenol and flavonoids content was evaluated in addition to antioxidant activity as shown by Fe2+ chelation, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability, and 2,2-azino-bis-(3-ethylbenthiazoline-6-sulfonic acid) (ABTS) radical scavenging ability.
29381472	4	51	from	activities	994:1003	arg1	BChE					1017:1020	BChE	1017:1020	BChE	1017:1020	Inhibitory activities on AChE and BChE were evaluated.
29381472	4	51	from	activities	994:1003	arg1	AChE					1008:1011	AChE	1008:1011	AChE	1008:1011	Inhibitory activities on AChE and BChE were evaluated.
29381472	8	52	theme	stem	1604:1607	arg1	bark					1609:1612	the stem bark	1600:1612	the stem bark	1600:1612	CONCLUSIONS Hence, possible mechanism through which the stem bark executes their anti-Alzheimer's disease activity might be by inhibiting cholinesterase activities in addition to suppressing oxidative-stress-induced neurodegeneration.
29381472	0	53	theme	Baill	222:226	arg1	bark					228:231	Irvingia gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark	175:231	Irvingia gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark	175:231	Chromatographic fingerprint analysis, antioxidant properties, and inhibition of cholinergic enzymes (acetylcholinesterase and butyrylcholinesterase) of phenolic extracts from Irvingia gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark.
29381472	6	54	theme	phenolic	1170:1177	arg1	extracts					1179:1186	The phenolic extracts	1166:1186	The phenolic extracts	1166:1186	The phenolic extracts displayed DPPH radical scavenging ability (IC50=19.98 μg/mL), ABTS radical scavenging ability (IC50=18.25 μg/mL), iron chelation (IC50=113.10 μg/mL), and reducing power (Fe3+ to Fe2+) (5.94 mg ascorbic acid equivalent/100 g).
29381472	6	55	theme	radical	1255:1261	arg1	ability					1274:1280	ABTS radical scavenging ability	1250:1280	ABTS radical scavenging ability (IC50=18.25 μg/mL)	1250:1299	The phenolic extracts displayed DPPH radical scavenging ability (IC50=19.98 μg/mL), ABTS radical scavenging ability (IC50=18.25 μg/mL), iron chelation (IC50=113.10 μg/mL), and reducing power (Fe3+ to Fe2+) (5.94 mg ascorbic acid equivalent/100 g).
29381472	6	55	theme	radical	1255:1261	arg1	μg/mL					1294:1298	IC50=18.25 μg/mL	1283:1298	IC50=18.25 μg/mL	1283:1298	The phenolic extracts displayed DPPH radical scavenging ability (IC50=19.98 μg/mL), ABTS radical scavenging ability (IC50=18.25 μg/mL), iron chelation (IC50=113.10 μg/mL), and reducing power (Fe3+ to Fe2+) (5.94 mg ascorbic acid equivalent/100 g).
29381472	8	56	theme	cholinesterase	1686:1699	arg1	activities					1701:1710	cholinesterase activities	1686:1710	cholinesterase activities	1686:1710	CONCLUSIONS Hence, possible mechanism through which the stem bark executes their anti-Alzheimer's disease activity might be by inhibiting cholinesterase activities in addition to suppressing oxidative-stress-induced neurodegeneration.
29381472	3	57	theme	antioxidant	777:787	arg1	activity					789:796	antioxidant activity	777:796	antioxidant activity as shown by Fe2+ chelation	777:823	METHODS Total phenol and flavonoids content was evaluated in addition to antioxidant activity as shown by Fe2+ chelation, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability, and 2,2-azino-bis-(3-ethylbenthiazoline-6-sulfonic acid) (ABTS) radical scavenging ability.
29381472	0	58	theme	extracts	161:168	arg1	analysis					28:35	Chromatographic fingerprint analysis	0:35	Chromatographic fingerprint analysis	0:35	Chromatographic fingerprint analysis, antioxidant properties, and inhibition of cholinergic enzymes (acetylcholinesterase and butyrylcholinesterase) of phenolic extracts from Irvingia gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark.
29381472	0	58	theme	extracts	161:168	arg1	properties					50:59	antioxidant properties	38:59	antioxidant properties	38:59	Chromatographic fingerprint analysis, antioxidant properties, and inhibition of cholinergic enzymes (acetylcholinesterase and butyrylcholinesterase) of phenolic extracts from Irvingia gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark.
29381472	0	58	theme	extracts	161:168	arg1	inhibition					66:75	inhibition	66:75	inhibition of cholinergic enzymes	66:98	Chromatographic fingerprint analysis, antioxidant properties, and inhibition of cholinergic enzymes (acetylcholinesterase and butyrylcholinesterase) of phenolic extracts from Irvingia gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark.
29381472	5	59	theme	ellagic	1097:1103	arg1	acid					1091:1094	phenolic acid	1082:1094	phenolic acid (ellagic acid)	1082:1109	RESULTS The extract was found to be rich in phenolic acid (ellagic acid) and flavonoids (quercetrin, kaempferol, and apigenin).
29381472	5	59	theme	ellagic	1097:1103	arg1	acid					1105:1108	ellagic acid	1097:1108	ellagic acid	1097:1108	RESULTS The extract was found to be rich in phenolic acid (ellagic acid) and flavonoids (quercetrin, kaempferol, and apigenin).
29381472	5	60	dep	RESULTS	1038:1044	arg1	found					1062:1066	found	1062:1066	was found to be rich in phenolic acid (ellagic acid) and flavonoids (quercetrin, kaempferol, and apigenin)	1058:1163	RESULTS The extract was found to be rich in phenolic acid (ellagic acid) and flavonoids (quercetrin, kaempferol, and apigenin).
29381472	1	61	theme	scientific	412:421	arg1	basis					423:427	scientific basis	412:427	scientific basis	412:427	BACKGROUND Irvingia gabonensis stem bark is a medicinal plant used in most parts of Africa to manage a number of ailments including neurodegenerative diseases that occur without scientific basis.
29381472	8	62	theme	oxidative-stress-induced	1739:1762	arg1	neurodegeneration					1764:1780	oxidative-stress-induced neurodegeneration	1739:1780	oxidative-stress-induced neurodegeneration	1739:1780	CONCLUSIONS Hence, possible mechanism through which the stem bark executes their anti-Alzheimer's disease activity might be by inhibiting cholinesterase activities in addition to suppressing oxidative-stress-induced neurodegeneration.
29381472	6	63	theme	iron	1302:1305	arg1	chelation					1307:1315	iron chelation	1302:1315	iron chelation (IC50=113.10 μg/mL)	1302:1335	The phenolic extracts displayed DPPH radical scavenging ability (IC50=19.98 μg/mL), ABTS radical scavenging ability (IC50=18.25 μg/mL), iron chelation (IC50=113.10 μg/mL), and reducing power (Fe3+ to Fe2+) (5.94 mg ascorbic acid equivalent/100 g).
29381472	6	63	theme	iron	1302:1305	arg1	μg/mL					1330:1334	IC50=113.10 μg/mL	1318:1334	IC50=113.10 μg/mL	1318:1334	The phenolic extracts displayed DPPH radical scavenging ability (IC50=19.98 μg/mL), ABTS radical scavenging ability (IC50=18.25 μg/mL), iron chelation (IC50=113.10 μg/mL), and reducing power (Fe3+ to Fe2+) (5.94 mg ascorbic acid equivalent/100 g).
29381472	3	64	theme	2,2-diphenyl-1-picrylhydrazyl	826:854	arg1	ability					882:888	2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability	826:888	2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability	826:888	METHODS Total phenol and flavonoids content was evaluated in addition to antioxidant activity as shown by Fe2+ chelation, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability, and 2,2-azino-bis-(3-ethylbenthiazoline-6-sulfonic acid) (ABTS) radical scavenging ability.
29381472	6	65	theme	radical	1203:1209	arg1	ability					1222:1228	DPPH radical scavenging ability	1198:1228	DPPH radical scavenging ability (IC50=19.98 μg/mL)	1198:1247	The phenolic extracts displayed DPPH radical scavenging ability (IC50=19.98 μg/mL), ABTS radical scavenging ability (IC50=18.25 μg/mL), iron chelation (IC50=113.10 μg/mL), and reducing power (Fe3+ to Fe2+) (5.94 mg ascorbic acid equivalent/100 g).
29381472	6	65	theme	radical	1203:1209	arg1	μg/mL					1242:1246	IC50=19.98 μg/mL	1231:1246	IC50=19.98 μg/mL	1231:1246	The phenolic extracts displayed DPPH radical scavenging ability (IC50=19.98 μg/mL), ABTS radical scavenging ability (IC50=18.25 μg/mL), iron chelation (IC50=113.10 μg/mL), and reducing power (Fe3+ to Fe2+) (5.94 mg ascorbic acid equivalent/100 g).
29381472	0	66	theme	enzymes	92:98	arg1	analysis					28:35	Chromatographic fingerprint analysis	0:35	Chromatographic fingerprint analysis	0:35	Chromatographic fingerprint analysis, antioxidant properties, and inhibition of cholinergic enzymes (acetylcholinesterase and butyrylcholinesterase) of phenolic extracts from Irvingia gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark.
29381472	0	66	theme	enzymes	92:98	arg1	properties					50:59	antioxidant properties	38:59	antioxidant properties	38:59	Chromatographic fingerprint analysis, antioxidant properties, and inhibition of cholinergic enzymes (acetylcholinesterase and butyrylcholinesterase) of phenolic extracts from Irvingia gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark.
29381472	0	66	theme	enzymes	92:98	arg1	inhibition					66:75	inhibition	66:75	inhibition of cholinergic enzymes	66:98	Chromatographic fingerprint analysis, antioxidant properties, and inhibition of cholinergic enzymes (acetylcholinesterase and butyrylcholinesterase) of phenolic extracts from Irvingia gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark.
29381472	2	67	dep	Aubry-Lecomte	666:678	arg1	Rorke					685:689	Rorke	685:689	Rorke	685:689	This work characterized the phenolic composition, evaluated the cholinergic enzymes (acetylcholinesterase, AChE and butyrylcholinesterase, BChE) inhibition, and assessed the antioxidant activity of phenolic extracts from I. gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark.
29381472	2	67	dep	Aubry-Lecomte	666:678	arg1	ex					680:681	Aubry-Lecomte ex	666:681	Aubry-Lecomte ex O'Rorke	666:689	This work characterized the phenolic composition, evaluated the cholinergic enzymes (acetylcholinesterase, AChE and butyrylcholinesterase, BChE) inhibition, and assessed the antioxidant activity of phenolic extracts from I. gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark.
29381472	2	68	theme	enzymes	506:512	arg1	inhibition					575:584	the cholinergic enzymes (acetylcholinesterase, AChE and butyrylcholinesterase, BChE) inhibition	490:584	the cholinergic enzymes (acetylcholinesterase, AChE and butyrylcholinesterase, BChE) inhibition	490:584	This work characterized the phenolic composition, evaluated the cholinergic enzymes (acetylcholinesterase, AChE and butyrylcholinesterase, BChE) inhibition, and assessed the antioxidant activity of phenolic extracts from I. gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark.
29381472	8	69	dep	suppressing	1727:1737	arg1	addition					1715:1722	addition	1715:1722	addition	1715:1722	CONCLUSIONS Hence, possible mechanism through which the stem bark executes their anti-Alzheimer's disease activity might be by inhibiting cholinesterase activities in addition to suppressing oxidative-stress-induced neurodegeneration.
29381472	2	70	from	bark	698:701	arg1	activity					616:623	the antioxidant activity	600:623	the antioxidant activity of phenolic extracts from I. gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark	600:701	This work characterized the phenolic composition, evaluated the cholinergic enzymes (acetylcholinesterase, AChE and butyrylcholinesterase, BChE) inhibition, and assessed the antioxidant activity of phenolic extracts from I. gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark.
29381472	2	70	from	bark	698:701	arg1	extracts					637:644	phenolic extracts	628:644	phenolic extracts from I. gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark	628:701	This work characterized the phenolic composition, evaluated the cholinergic enzymes (acetylcholinesterase, AChE and butyrylcholinesterase, BChE) inhibition, and assessed the antioxidant activity of phenolic extracts from I. gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark.
29381472	3	71	theme	radical	863:869	arg1	ability					882:888	2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability	826:888	2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability	826:888	METHODS Total phenol and flavonoids content was evaluated in addition to antioxidant activity as shown by Fe2+ chelation, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability, and 2,2-azino-bis-(3-ethylbenthiazoline-6-sulfonic acid) (ABTS) radical scavenging ability.
29381472	4	72	theme	Inhibitory	983:992	arg1	activities					994:1003	Inhibitory activities	983:1003	Inhibitory activities on AChE and BChE	983:1020	Inhibitory activities on AChE and BChE were evaluated.
29381472	3	73	theme	DPPH	857:860	arg1	ability					882:888	2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability	826:888	2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability	826:888	METHODS Total phenol and flavonoids content was evaluated in addition to antioxidant activity as shown by Fe2+ chelation, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability, and 2,2-azino-bis-(3-ethylbenthiazoline-6-sulfonic acid) (ABTS) radical scavenging ability.
29381472	6	74	theme	reducing	1342:1349	arg1	power					1351:1355	reducing power	1342:1355	reducing power (Fe3+ to Fe2+) (5.94 mg ascorbic acid equivalent/100 g)	1342:1411	The phenolic extracts displayed DPPH radical scavenging ability (IC50=19.98 μg/mL), ABTS radical scavenging ability (IC50=18.25 μg/mL), iron chelation (IC50=113.10 μg/mL), and reducing power (Fe3+ to Fe2+) (5.94 mg ascorbic acid equivalent/100 g).
29381472	6	74	theme	reducing	1342:1349	arg1	g					1410:1410	5.94 mg ascorbic acid equivalent/100 g	1373:1410	5.94 mg ascorbic acid equivalent/100 g	1373:1410	The phenolic extracts displayed DPPH radical scavenging ability (IC50=19.98 μg/mL), ABTS radical scavenging ability (IC50=18.25 μg/mL), iron chelation (IC50=113.10 μg/mL), and reducing power (Fe3+ to Fe2+) (5.94 mg ascorbic acid equivalent/100 g).
29381472	0	75	from	properties	50:59	arg1	bark					228:231	Irvingia gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark	175:231	Irvingia gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark	175:231	Chromatographic fingerprint analysis, antioxidant properties, and inhibition of cholinergic enzymes (acetylcholinesterase and butyrylcholinesterase) of phenolic extracts from Irvingia gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark.
29381472	0	76	theme	fingerprint	16:26	arg1	analysis					28:35	Chromatographic fingerprint analysis	0:35	Chromatographic fingerprint analysis	0:35	Chromatographic fingerprint analysis, antioxidant properties, and inhibition of cholinergic enzymes (acetylcholinesterase and butyrylcholinesterase) of phenolic extracts from Irvingia gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark.
29381472	3	77	dep	activity	789:796	arg1	shown					801:805	shown	801:805	shown by Fe2+ chelation	801:823	METHODS Total phenol and flavonoids content was evaluated in addition to antioxidant activity as shown by Fe2+ chelation, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability, and 2,2-azino-bis-(3-ethylbenthiazoline-6-sulfonic acid) (ABTS) radical scavenging ability.
29381472	2	78	theme	phenolic	458:465	arg1	composition					467:477	the phenolic composition	454:477	the phenolic composition	454:477	This work characterized the phenolic composition, evaluated the cholinergic enzymes (acetylcholinesterase, AChE and butyrylcholinesterase, BChE) inhibition, and assessed the antioxidant activity of phenolic extracts from I. gabonensis (Aubry-Lecomte ex O'Rorke) Baill bark.
29381472	8	79	dep	CONCLUSIONS	1548:1558	arg1	inhibiting					1675:1684	inhibiting	1675:1684	inhibiting cholinesterase activities in addition to suppressing oxidative-stress-induced neurodegeneration	1675:1780	CONCLUSIONS Hence, possible mechanism through which the stem bark executes their anti-Alzheimer's disease activity might be by inhibiting cholinesterase activities in addition to suppressing oxidative-stress-induced neurodegeneration.
29381472	6	80	theme	equivalent/100	1395:1408	arg1	power					1351:1355	reducing power	1342:1355	reducing power (Fe3+ to Fe2+) (5.94 mg ascorbic acid equivalent/100 g)	1342:1411	The phenolic extracts displayed DPPH radical scavenging ability (IC50=19.98 μg/mL), ABTS radical scavenging ability (IC50=18.25 μg/mL), iron chelation (IC50=113.10 μg/mL), and reducing power (Fe3+ to Fe2+) (5.94 mg ascorbic acid equivalent/100 g).
29381472	6	80	theme	equivalent/100	1395:1408	arg1	g					1410:1410	5.94 mg ascorbic acid equivalent/100 g	1373:1410	5.94 mg ascorbic acid equivalent/100 g	1373:1410	The phenolic extracts displayed DPPH radical scavenging ability (IC50=19.98 μg/mL), ABTS radical scavenging ability (IC50=18.25 μg/mL), iron chelation (IC50=113.10 μg/mL), and reducing power (Fe3+ to Fe2+) (5.94 mg ascorbic acid equivalent/100 g).
29381472	6	81	theme	ascorbic	1381:1388	arg1	power					1351:1355	reducing power	1342:1355	reducing power (Fe3+ to Fe2+) (5.94 mg ascorbic acid equivalent/100 g)	1342:1411	The phenolic extracts displayed DPPH radical scavenging ability (IC50=19.98 μg/mL), ABTS radical scavenging ability (IC50=18.25 μg/mL), iron chelation (IC50=113.10 μg/mL), and reducing power (Fe3+ to Fe2+) (5.94 mg ascorbic acid equivalent/100 g).
29381472	6	81	theme	ascorbic	1381:1388	arg1	g					1410:1410	5.94 mg ascorbic acid equivalent/100 g	1373:1410	5.94 mg ascorbic acid equivalent/100 g	1373:1410	The phenolic extracts displayed DPPH radical scavenging ability (IC50=19.98 μg/mL), ABTS radical scavenging ability (IC50=18.25 μg/mL), iron chelation (IC50=113.10 μg/mL), and reducing power (Fe3+ to Fe2+) (5.94 mg ascorbic acid equivalent/100 g).
29381472	1	82	theme	most	304:307	arg1	parts					309:313	most parts	304:313	most parts of Africa	304:323	BACKGROUND Irvingia gabonensis stem bark is a medicinal plant used in most parts of Africa to manage a number of ailments including neurodegenerative diseases that occur without scientific basis.
29381472	3	83	theme	scavenging	963:972	arg1	ability					974:980	2,2-azino-bis-(3-ethylbenthiazoline-6-sulfonic acid) (ABTS) radical scavenging ability	895:980	2,2-azino-bis-(3-ethylbenthiazoline-6-sulfonic acid) (ABTS) radical scavenging ability	895:980	METHODS Total phenol and flavonoids content was evaluated in addition to antioxidant activity as shown by Fe2+ chelation, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability, and 2,2-azino-bis-(3-ethylbenthiazoline-6-sulfonic acid) (ABTS) radical scavenging ability.
29381472	6	84	theme	5.94	1373:1376	arg1	mg					1378:1379	mg	1378:1379	mg	1378:1379	The phenolic extracts displayed DPPH radical scavenging ability (IC50=19.98 μg/mL), ABTS radical scavenging ability (IC50=18.25 μg/mL), iron chelation (IC50=113.10 μg/mL), and reducing power (Fe3+ to Fe2+) (5.94 mg ascorbic acid equivalent/100 g).
29381472	8	85	theme	possible	1567:1574	arg1	mechanism					1576:1584	possible mechanism	1567:1584	possible mechanism through which the stem bark executes their anti-Alzheimer's disease activity	1567:1661	CONCLUSIONS Hence, possible mechanism through which the stem bark executes their anti-Alzheimer's disease activity might be by inhibiting cholinesterase activities in addition to suppressing oxidative-stress-induced neurodegeneration.
29381472	1	86	theme	Irvingia	245:252	arg1	plant					290:294	a medicinal plant	278:294	a medicinal plant used in most parts of Africa to manage a number of ailments including neurodegenerative diseases that occur without scientific basis	278:427	BACKGROUND Irvingia gabonensis stem bark is a medicinal plant used in most parts of Africa to manage a number of ailments including neurodegenerative diseases that occur without scientific basis.
29381472	1	86	theme	Irvingia	245:252	arg1	bark					270:273	BACKGROUND Irvingia gabonensis stem bark	234:273	BACKGROUND Irvingia gabonensis stem bark	234:273	BACKGROUND Irvingia gabonensis stem bark is a medicinal plant used in most parts of Africa to manage a number of ailments including neurodegenerative diseases that occur without scientific basis.
29381472	1	87	theme	stem	265:268	arg1	plant					290:294	a medicinal plant	278:294	a medicinal plant used in most parts of Africa to manage a number of ailments including neurodegenerative diseases that occur without scientific basis	278:427	BACKGROUND Irvingia gabonensis stem bark is a medicinal plant used in most parts of Africa to manage a number of ailments including neurodegenerative diseases that occur without scientific basis.
29381472	1	87	theme	stem	265:268	arg1	bark					270:273	BACKGROUND Irvingia gabonensis stem bark	234:273	BACKGROUND Irvingia gabonensis stem bark	234:273	BACKGROUND Irvingia gabonensis stem bark is a medicinal plant used in most parts of Africa to manage a number of ailments including neurodegenerative diseases that occur without scientific basis.
28777137	2	0	theme	Beet	361:364	arg1	[Chenopodiaceae					366:380	Beet [Chenopodiaceae	361:380	Beet [Chenopodiaceae	361:380	Herein, for the first time, electrospinning setup was proposed to fabricate an efficient composite nanofibrous structure of Beta vulgaris (obtained from Beet [Chenopodiaceae or Amaranthaceae]) belonged to polysaccharides and an elastic polymer named nylon 66 for skin tissue engineering.
28777137	4	1	contain	have	742:745	arg1	examinations					729:740	Scanning electron microscope examinations	700:740	Scanning electron microscope examinations	700:740	Scanning electron microscope examinations have approved the uniform and homogeneous structure of composite nanofibers containing nylon polymer and B. vulgaris extract.
28777137	4	1	contain	have	742:745	arg2	structure					784:792	the uniform and homogeneous structure	756:792	the uniform and homogeneous structure of composite nanofibers containing nylon polymer and B. vulgaris extract	756:865	Scanning electron microscope examinations have approved the uniform and homogeneous structure of composite nanofibers containing nylon polymer and B. vulgaris extract.
28777137	2	2	theme	efficient	287:295	arg1	structure					319:327	an efficient composite nanofibrous structure	284:327	an efficient composite nanofibrous structure of Beta vulgaris (obtained from Beet [Chenopodiaceae or Amaranthaceae]) belonged to polysaccharides and an elastic polymer named nylon 66	284:465	Herein, for the first time, electrospinning setup was proposed to fabricate an efficient composite nanofibrous structure of Beta vulgaris (obtained from Beet [Chenopodiaceae or Amaranthaceae]) belonged to polysaccharides and an elastic polymer named nylon 66 for skin tissue engineering.
28777137	10	3	theme	original	1844:1851	arg1	property					1853:1860	the original property	1840:1860	the original property of keratinocytes	1840:1877	Furthermore, immunocytochemistry results (cytokeratin 10 and loricrin) approved that the original property of keratinocytes was strongly preserved using composite scaffold.
28777137	1	4	theme	skin	195:198	arg1	tissue					200:205	skin tissue	195:205	skin tissue	195:205	Natural compounds containing polysaccharide ingredients have been employed as candidates for treatment of skin tissue.
28777137	7	5	contain	containing	1236:1245	arg1	sample					1229:1234	composite sample	1219:1234	composite sample containing B. vulgaris extract	1219:1265	To our surprise, it was found that compared with nylon nanofibrous scaffold, composite sample containing B. vulgaris extract has lower contact angle indicating a higher hydrophilic surface.
28777137	7	5	contain	containing	1236:1245	arg2	extract					1259:1265	B. vulgaris extract	1247:1265	B. vulgaris extract	1247:1265	To our surprise, it was found that compared with nylon nanofibrous scaffold, composite sample containing B. vulgaris extract has lower contact angle indicating a higher hydrophilic surface.
28777137	3	6	dep	Fourier	615:621	arg1	transform					623:631	transform	623:631	transform infrared (FTIR) spectroscopy, mechanical assay, and contact angle	623:697	Both prepared scaffolds including noncomposite and composite types were studied by Scanning electron microscope (SEM), Fourier transform infrared (FTIR) spectroscopy, mechanical assay, and contact angle.
28777137	6	7	theme	property	1008:1015	arg1	measurement					982:992	measurement	982:992	measurement of mechanical property with cell-laden composite scaffolds	982:1051	Also, measurement of mechanical property with cell-laden composite scaffolds approved the desirable similarity between corresponding scaffold and native skin tissue.
28777137	10	8	theme	composite	1908:1916	arg1	scaffold					1918:1925	composite scaffold	1908:1925	composite scaffold	1908:1925	Furthermore, immunocytochemistry results (cytokeratin 10 and loricrin) approved that the original property of keratinocytes was strongly preserved using composite scaffold.
28777137	6	9	with	measurement	982:992	arg1	scaffolds					1043:1051	cell-laden composite scaffolds	1022:1051	cell-laden composite scaffolds	1022:1051	Also, measurement of mechanical property with cell-laden composite scaffolds approved the desirable similarity between corresponding scaffold and native skin tissue.
28777137	2	10	theme	nanofibrous	307:317	arg1	structure					319:327	an efficient composite nanofibrous structure	284:327	an efficient composite nanofibrous structure of Beta vulgaris (obtained from Beet [Chenopodiaceae or Amaranthaceae]) belonged to polysaccharides and an elastic polymer named nylon 66	284:465	Herein, for the first time, electrospinning setup was proposed to fabricate an efficient composite nanofibrous structure of Beta vulgaris (obtained from Beet [Chenopodiaceae or Amaranthaceae]) belonged to polysaccharides and an elastic polymer named nylon 66 for skin tissue engineering.
28777137	8	11	theme	seeding	1343:1349	arg1	process					1351:1357	cell seeding process	1338:1357	cell seeding process of keratinocyte cells on composite and noncomposite scaffolds	1338:1419	After cell seeding process of keratinocyte cells on composite and noncomposite scaffolds, SEM and 3[4,5-dimethylthiazoyl-2-yl]-2,5 diphenyltetrazolium bromide (MTT) assays approved higher number of attached cells onto the corresponding composite electrospun membrane.
28777137	11	12	theme	skin	2091:2094	arg1	tissue					2096:2101	native skin tissue	2084:2101	native skin tissue	2084:2101	The corresponding study tries to introduce a new type of natural-based scaffolds for dermal tissue engineering that exhibits an elastic behavior similar to native skin tissue.
28777137	8	13	theme	cells	1539:1543	arg1	number					1520:1525	higher number	1513:1525	higher number of attached cells onto the corresponding composite electrospun membrane	1513:1597	After cell seeding process of keratinocyte cells on composite and noncomposite scaffolds, SEM and 3[4,5-dimethylthiazoyl-2-yl]-2,5 diphenyltetrazolium bromide (MTT) assays approved higher number of attached cells onto the corresponding composite electrospun membrane.
28777137	11	14	theme	corresponding	1932:1944	arg1	study					1946:1950	The corresponding study	1928:1950	The corresponding study	1928:1950	The corresponding study tries to introduce a new type of natural-based scaffolds for dermal tissue engineering that exhibits an elastic behavior similar to native skin tissue.
28777137	2	15	theme	first	224:228	arg1	time					230:233	the first time	220:233	the first time	220:233	Herein, for the first time, electrospinning setup was proposed to fabricate an efficient composite nanofibrous structure of Beta vulgaris (obtained from Beet [Chenopodiaceae or Amaranthaceae]) belonged to polysaccharides and an elastic polymer named nylon 66 for skin tissue engineering.
28777137	8	16	theme	cells	1375:1379	arg1	process					1351:1357	cell seeding process	1338:1357	cell seeding process of keratinocyte cells on composite and noncomposite scaffolds	1338:1419	After cell seeding process of keratinocyte cells on composite and noncomposite scaffolds, SEM and 3[4,5-dimethylthiazoyl-2-yl]-2,5 diphenyltetrazolium bromide (MTT) assays approved higher number of attached cells onto the corresponding composite electrospun membrane.
28777137	8	17	theme	diphenyltetrazolium	1463:1481	arg1	MTT					1492:1494	MTT	1492:1494	MTT	1492:1494	After cell seeding process of keratinocyte cells on composite and noncomposite scaffolds, SEM and 3[4,5-dimethylthiazoyl-2-yl]-2,5 diphenyltetrazolium bromide (MTT) assays approved higher number of attached cells onto the corresponding composite electrospun membrane.
28777137	8	17	theme	diphenyltetrazolium	1463:1481	arg1	bromide					1483:1489	SEM and 3[4,5-dimethylthiazoyl-2-yl]-2,5 diphenyltetrazolium bromide	1422:1489	bromide	1483:1489	After cell seeding process of keratinocyte cells on composite and noncomposite scaffolds, SEM and 3[4,5-dimethylthiazoyl-2-yl]-2,5 diphenyltetrazolium bromide (MTT) assays approved higher number of attached cells onto the corresponding composite electrospun membrane.
28777137	3	18	theme	FTIR	643:646	arg1	spectroscopy					649:660	(FTIR) spectroscopy	642:660	(FTIR) spectroscopy	642:660	Both prepared scaffolds including noncomposite and composite types were studied by Scanning electron microscope (SEM), Fourier transform infrared (FTIR) spectroscopy, mechanical assay, and contact angle.
28777137	5	19	theme	nanofibers	964:973	arg1	mat					957:959	the interwoven mat	942:959	the interwoven mat of nanofibers	942:973	FTIR spectroscopy was endorsed the presence of B. vulgaris extract within the interwoven mat of nanofibers.
28777137	8	20	theme	composite	1568:1576	arg1	membrane					1590:1597	the corresponding composite electrospun membrane	1550:1597	the corresponding composite electrospun membrane	1550:1597	After cell seeding process of keratinocyte cells on composite and noncomposite scaffolds, SEM and 3[4,5-dimethylthiazoyl-2-yl]-2,5 diphenyltetrazolium bromide (MTT) assays approved higher number of attached cells onto the corresponding composite electrospun membrane.
28777137	11	21	theme	elastic	2056:2062	arg1	behavior					2064:2071	an elastic behavior	2053:2071	an elastic behavior similar to native skin tissue	2053:2101	The corresponding study tries to introduce a new type of natural-based scaffolds for dermal tissue engineering that exhibits an elastic behavior similar to native skin tissue.
28777137	8	22	theme	composite	1384:1392	arg1	scaffolds					1411:1419	composite and noncomposite scaffolds	1384:1419	composite and noncomposite scaffolds	1384:1419	After cell seeding process of keratinocyte cells on composite and noncomposite scaffolds, SEM and 3[4,5-dimethylthiazoyl-2-yl]-2,5 diphenyltetrazolium bromide (MTT) assays approved higher number of attached cells onto the corresponding composite electrospun membrane.
28777137	4	23	contain	containing	818:827	arg2	polymer					835:841	nylon polymer	829:841	nylon polymer	829:841	Scanning electron microscope examinations have approved the uniform and homogeneous structure of composite nanofibers containing nylon polymer and B. vulgaris extract.
28777137	4	23	contain	containing	818:827	arg2	extract					859:865	B. vulgaris extract	847:865	B. vulgaris extract	847:865	Scanning electron microscope examinations have approved the uniform and homogeneous structure of composite nanofibers containing nylon polymer and B. vulgaris extract.
28777137	4	23	contain	containing	818:827	arg1	nanofibers					807:816	composite nanofibers	797:816	composite nanofibers containing nylon polymer and B. vulgaris extract	797:865	Scanning electron microscope examinations have approved the uniform and homogeneous structure of composite nanofibers containing nylon polymer and B. vulgaris extract.
28777137	5	24	theme	extract	927:933	arg1	presence					903:910	the presence	899:910	the presence of B. vulgaris extract within the interwoven mat of nanofibers	899:973	FTIR spectroscopy was endorsed the presence of B. vulgaris extract within the interwoven mat of nanofibers.
28777137	4	25	theme	electron	709:716	arg1	examinations					729:740	Scanning electron microscope examinations	700:740	Scanning electron microscope examinations	700:740	Scanning electron microscope examinations have approved the uniform and homogeneous structure of composite nanofibers containing nylon polymer and B. vulgaris extract.
28777137	0	26	theme	Vulgaris	79:86	arg1	Electrospinning					49:63	Electrospinning	49:63	Electrospinning of Nylon-Beta Vulgaris	49:86	Natural Compounds for Skin Tissue Engineering by Electrospinning of Nylon-Beta Vulgaris.
28777137	10	27	dep	approved	1826:1833	arg1	cytokeratin					1797:1807	cytokeratin 10	1797:1810	cytokeratin 10	1797:1810	Furthermore, immunocytochemistry results (cytokeratin 10 and loricrin) approved that the original property of keratinocytes was strongly preserved using composite scaffold.
28777137	10	27	dep	approved	1826:1833	arg1	loricrin					1816:1823	loricrin	1816:1823	loricrin	1816:1823	Furthermore, immunocytochemistry results (cytokeratin 10 and loricrin) approved that the original property of keratinocytes was strongly preserved using composite scaffold.
28777137	6	28	theme	cell-laden	1022:1031	arg1	scaffolds					1043:1051	cell-laden composite scaffolds	1022:1051	cell-laden composite scaffolds	1022:1051	Also, measurement of mechanical property with cell-laden composite scaffolds approved the desirable similarity between corresponding scaffold and native skin tissue.
28777137	4	29	theme	nanofibers	807:816	arg1	structure					784:792	the uniform and homogeneous structure	756:792	the uniform and homogeneous structure of composite nanofibers containing nylon polymer and B. vulgaris extract	756:865	Scanning electron microscope examinations have approved the uniform and homogeneous structure of composite nanofibers containing nylon polymer and B. vulgaris extract.
28777137	4	30	theme	uniform	760:766	arg1	structure					784:792	the uniform and homogeneous structure	756:792	the uniform and homogeneous structure of composite nanofibers containing nylon polymer and B. vulgaris extract	756:865	Scanning electron microscope examinations have approved the uniform and homogeneous structure of composite nanofibers containing nylon polymer and B. vulgaris extract.
28777137	11	31	theme	dermal	2013:2018	arg1	engineering					2027:2037	dermal tissue engineering	2013:2037	dermal tissue engineering	2013:2037	The corresponding study tries to introduce a new type of natural-based scaffolds for dermal tissue engineering that exhibits an elastic behavior similar to native skin tissue.
28777137	0	32	theme	Natural	0:6	arg1	Compounds					8:16	Natural Compounds	0:16	Natural Compounds for Skin Tissue Engineering by Electrospinning of Nylon-Beta Vulgaris	0:86	Natural Compounds for Skin Tissue Engineering by Electrospinning of Nylon-Beta Vulgaris.
28777137	4	33	dep	have	742:745	arg1	approved					747:754	approved	747:754	approved	747:754	Scanning electron microscope examinations have approved the uniform and homogeneous structure of composite nanofibers containing nylon polymer and B. vulgaris extract.
28777137	6	34	theme	skin	1129:1132	arg1	tissue					1134:1139	native skin tissue	1122:1139	native skin tissue	1122:1139	Also, measurement of mechanical property with cell-laden composite scaffolds approved the desirable similarity between corresponding scaffold and native skin tissue.
28777137	4	35	theme	homogeneous	772:782	arg1	structure					784:792	the uniform and homogeneous structure	756:792	the uniform and homogeneous structure of composite nanofibers containing nylon polymer and B. vulgaris extract	756:865	Scanning electron microscope examinations have approved the uniform and homogeneous structure of composite nanofibers containing nylon polymer and B. vulgaris extract.
28777137	2	36	theme	elastic	436:442	arg1	polymer					444:450	an elastic polymer	433:450	an elastic polymer named nylon 66	433:465	Herein, for the first time, electrospinning setup was proposed to fabricate an efficient composite nanofibrous structure of Beta vulgaris (obtained from Beet [Chenopodiaceae or Amaranthaceae]) belonged to polysaccharides and an elastic polymer named nylon 66 for skin tissue engineering.
28777137	7	37	theme	composite	1219:1227	arg1	sample					1229:1234	composite sample	1219:1234	composite sample containing B. vulgaris extract	1219:1265	To our surprise, it was found that compared with nylon nanofibrous scaffold, composite sample containing B. vulgaris extract has lower contact angle indicating a higher hydrophilic surface.
28777137	1	38	theme	polysaccharide	118:131	arg1	ingredients					133:143	polysaccharide ingredients	118:143	polysaccharide ingredients	118:143	Natural compounds containing polysaccharide ingredients have been employed as candidates for treatment of skin tissue.
28777137	3	39	theme	mechanical	663:672	arg1	assay					674:678	mechanical assay	663:678	mechanical assay	663:678	Both prepared scaffolds including noncomposite and composite types were studied by Scanning electron microscope (SEM), Fourier transform infrared (FTIR) spectroscopy, mechanical assay, and contact angle.
28777137	4	40	theme	vulgaris	850:857	arg1	extract					859:865	B. vulgaris extract	847:865	B. vulgaris extract	847:865	Scanning electron microscope examinations have approved the uniform and homogeneous structure of composite nanofibers containing nylon polymer and B. vulgaris extract.
28777137	0	41	theme	Tissue	27:32	arg1	Engineering					34:44	Skin Tissue Engineering	22:44	Skin Tissue Engineering by Electrospinning of Nylon-Beta Vulgaris	22:86	Natural Compounds for Skin Tissue Engineering by Electrospinning of Nylon-Beta Vulgaris.
28777137	6	42	theme	corresponding	1095:1107	arg1	scaffold					1109:1116	corresponding scaffold	1095:1116	corresponding scaffold	1095:1116	Also, measurement of mechanical property with cell-laden composite scaffolds approved the desirable similarity between corresponding scaffold and native skin tissue.
28777137	2	43	theme	skin	471:474	arg1	engineering					483:493	skin tissue engineering	471:493	skin tissue engineering	471:493	Herein, for the first time, electrospinning setup was proposed to fabricate an efficient composite nanofibrous structure of Beta vulgaris (obtained from Beet [Chenopodiaceae or Amaranthaceae]) belonged to polysaccharides and an elastic polymer named nylon 66 for skin tissue engineering.
28777137	7	44	dep	B.	1247:1248	arg1	vulgaris					1250:1257	vulgaris	1250:1257	vulgaris	1250:1257	To our surprise, it was found that compared with nylon nanofibrous scaffold, composite sample containing B. vulgaris extract has lower contact angle indicating a higher hydrophilic surface.
28777137	3	45	theme	prepared	501:508	arg1	noncomposite					530:541	noncomposite	530:541	noncomposite	530:541	Both prepared scaffolds including noncomposite and composite types were studied by Scanning electron microscope (SEM), Fourier transform infrared (FTIR) spectroscopy, mechanical assay, and contact angle.
28777137	3	45	theme	prepared	501:508	arg1	types					557:561	composite types	547:561	composite types	547:561	Both prepared scaffolds including noncomposite and composite types were studied by Scanning electron microscope (SEM), Fourier transform infrared (FTIR) spectroscopy, mechanical assay, and contact angle.
28777137	3	45	theme	prepared	501:508	arg1	scaffolds					510:518	Both prepared scaffolds	496:518	Both prepared scaffolds including noncomposite and composite types	496:561	Both prepared scaffolds including noncomposite and composite types were studied by Scanning electron microscope (SEM), Fourier transform infrared (FTIR) spectroscopy, mechanical assay, and contact angle.
28777137	7	46	theme	nylon	1191:1195	arg1	scaffold					1209:1216	nylon nanofibrous scaffold	1191:1216	nylon nanofibrous scaffold	1191:1216	To our surprise, it was found that compared with nylon nanofibrous scaffold, composite sample containing B. vulgaris extract has lower contact angle indicating a higher hydrophilic surface.
28777137	9	47	theme	polymerase	1712:1721	arg1	reaction					1729:1736	real-time polymerase chain reaction	1702:1736	real-time polymerase chain reaction (PCR) technique	1702:1752	Epidermal gene expression such as involucrin, cytokeratin 10, and cytokeratin 14 was observed through real-time polymerase chain reaction (PCR) technique.
28777137	9	47	theme	polymerase	1712:1721	arg1	PCR					1739:1741	PCR	1739:1741	PCR	1739:1741	Epidermal gene expression such as involucrin, cytokeratin 10, and cytokeratin 14 was observed through real-time polymerase chain reaction (PCR) technique.
28777137	3	48	theme	contact	685:691	arg1	angle					693:697	contact angle	685:697	contact angle	685:697	Both prepared scaffolds including noncomposite and composite types were studied by Scanning electron microscope (SEM), Fourier transform infrared (FTIR) spectroscopy, mechanical assay, and contact angle.
28777137	4	49	theme	nylon	829:833	arg1	polymer					835:841	nylon polymer	829:841	nylon polymer	829:841	Scanning electron microscope examinations have approved the uniform and homogeneous structure of composite nanofibers containing nylon polymer and B. vulgaris extract.
28777137	8	50	theme	bromide	1483:1489	arg1	assays					1497:1502	SEM and 3[4,5-dimethylthiazoyl-2-yl]-2,5 diphenyltetrazolium bromide (MTT) assays	1422:1502	SEM and 3[4,5-dimethylthiazoyl-2-yl]-2,5 diphenyltetrazolium bromide (MTT) assays	1422:1502	After cell seeding process of keratinocyte cells on composite and noncomposite scaffolds, SEM and 3[4,5-dimethylthiazoyl-2-yl]-2,5 diphenyltetrazolium bromide (MTT) assays approved higher number of attached cells onto the corresponding composite electrospun membrane.
28777137	9	51	theme	reaction	1729:1736	arg1	technique					1744:1752	real-time polymerase chain reaction (PCR) technique	1702:1752	real-time polymerase chain reaction (PCR) technique	1702:1752	Epidermal gene expression such as involucrin, cytokeratin 10, and cytokeratin 14 was observed through real-time polymerase chain reaction (PCR) technique.
28777137	11	52	theme	scaffolds	1999:2007	arg1	type					1977:1980	a new type	1971:1980	a new type of natural-based scaffolds for dermal tissue engineering that exhibits an elastic behavior similar to native skin tissue	1971:2101	The corresponding study tries to introduce a new type of natural-based scaffolds for dermal tissue engineering that exhibits an elastic behavior similar to native skin tissue.
28777137	7	53	theme	higher	1304:1309	arg1	surface					1323:1329	a higher hydrophilic surface	1302:1329	a higher hydrophilic surface	1302:1329	To our surprise, it was found that compared with nylon nanofibrous scaffold, composite sample containing B. vulgaris extract has lower contact angle indicating a higher hydrophilic surface.
28777137	2	54	theme	composite	297:305	arg1	structure					319:327	an efficient composite nanofibrous structure	284:327	an efficient composite nanofibrous structure of Beta vulgaris (obtained from Beet [Chenopodiaceae or Amaranthaceae]) belonged to polysaccharides and an elastic polymer named nylon 66	284:465	Herein, for the first time, electrospinning setup was proposed to fabricate an efficient composite nanofibrous structure of Beta vulgaris (obtained from Beet [Chenopodiaceae or Amaranthaceae]) belonged to polysaccharides and an elastic polymer named nylon 66 for skin tissue engineering.
28777137	8	55	theme	noncomposite	1398:1409	arg1	scaffolds					1411:1419	composite and noncomposite scaffolds	1384:1419	composite and noncomposite scaffolds	1384:1419	After cell seeding process of keratinocyte cells on composite and noncomposite scaffolds, SEM and 3[4,5-dimethylthiazoyl-2-yl]-2,5 diphenyltetrazolium bromide (MTT) assays approved higher number of attached cells onto the corresponding composite electrospun membrane.
28777137	11	56	theme	new	1973:1975	arg1	type					1977:1980	a new type	1971:1980	a new type of natural-based scaffolds for dermal tissue engineering that exhibits an elastic behavior similar to native skin tissue	1971:2101	The corresponding study tries to introduce a new type of natural-based scaffolds for dermal tissue engineering that exhibits an elastic behavior similar to native skin tissue.
28777137	10	57	theme	keratinocytes	1865:1877	arg1	property					1853:1860	the original property	1840:1860	the original property of keratinocytes	1840:1877	Furthermore, immunocytochemistry results (cytokeratin 10 and loricrin) approved that the original property of keratinocytes was strongly preserved using composite scaffold.
28777137	7	58	theme	contact	1277:1283	arg1	angle					1285:1289	lower contact angle	1271:1289	lower contact angle	1271:1289	To our surprise, it was found that compared with nylon nanofibrous scaffold, composite sample containing B. vulgaris extract has lower contact angle indicating a higher hydrophilic surface.
28777137	2	59	theme	Beta	332:335	arg1	vulgaris					337:344	Beta vulgaris	332:344	Beta vulgaris	332:344	Herein, for the first time, electrospinning setup was proposed to fabricate an efficient composite nanofibrous structure of Beta vulgaris (obtained from Beet [Chenopodiaceae or Amaranthaceae]) belonged to polysaccharides and an elastic polymer named nylon 66 for skin tissue engineering.
28777137	1	60	theme	tissue	200:205	arg1	treatment					182:190	treatment	182:190	treatment of skin tissue	182:205	Natural compounds containing polysaccharide ingredients have been employed as candidates for treatment of skin tissue.
28777137	3	61	theme	Scanning	579:586	arg1	SEM					609:611	SEM	609:611	SEM	609:611	Both prepared scaffolds including noncomposite and composite types were studied by Scanning electron microscope (SEM), Fourier transform infrared (FTIR) spectroscopy, mechanical assay, and contact angle.
28777137	3	61	theme	Scanning	579:586	arg1	Fourier					615:621	Fourier	615:621	Fourier transform infrared (FTIR) spectroscopy, mechanical assay, and contact angle	615:697	Both prepared scaffolds including noncomposite and composite types were studied by Scanning electron microscope (SEM), Fourier transform infrared (FTIR) spectroscopy, mechanical assay, and contact angle.
28777137	3	61	theme	Scanning	579:586	arg1	microscope					597:606	Scanning electron microscope	579:606	Scanning electron microscope (SEM)	579:612	Both prepared scaffolds including noncomposite and composite types were studied by Scanning electron microscope (SEM), Fourier transform infrared (FTIR) spectroscopy, mechanical assay, and contact angle.
28777137	5	62	theme	FTIR	868:871	arg1	spectroscopy					873:884	FTIR spectroscopy	868:884	FTIR spectroscopy	868:884	FTIR spectroscopy was endorsed the presence of B. vulgaris extract within the interwoven mat of nanofibers.
28777137	6	63	theme	mechanical	997:1006	arg1	property					1008:1015	mechanical property	997:1015	mechanical property	997:1015	Also, measurement of mechanical property with cell-laden composite scaffolds approved the desirable similarity between corresponding scaffold and native skin tissue.
28777137	8	64	theme	attached	1530:1537	arg1	cells					1539:1543	attached cells	1530:1543	attached cells	1530:1543	After cell seeding process of keratinocyte cells on composite and noncomposite scaffolds, SEM and 3[4,5-dimethylthiazoyl-2-yl]-2,5 diphenyltetrazolium bromide (MTT) assays approved higher number of attached cells onto the corresponding composite electrospun membrane.
28777137	2	65	theme	electrospinning	236:250	arg1	setup					252:256	electrospinning setup	236:256	electrospinning setup	236:256	Herein, for the first time, electrospinning setup was proposed to fabricate an efficient composite nanofibrous structure of Beta vulgaris (obtained from Beet [Chenopodiaceae or Amaranthaceae]) belonged to polysaccharides and an elastic polymer named nylon 66 for skin tissue engineering.
28777137	5	66	theme	B.	915:916	arg1	extract					927:933	B. vulgaris extract	915:933	B. vulgaris extract	915:933	FTIR spectroscopy was endorsed the presence of B. vulgaris extract within the interwoven mat of nanofibers.
28777137	8	67	theme	cell	1338:1341	arg1	process					1351:1357	cell seeding process	1338:1357	cell seeding process of keratinocyte cells on composite and noncomposite scaffolds	1338:1419	After cell seeding process of keratinocyte cells on composite and noncomposite scaffolds, SEM and 3[4,5-dimethylthiazoyl-2-yl]-2,5 diphenyltetrazolium bromide (MTT) assays approved higher number of attached cells onto the corresponding composite electrospun membrane.
28777137	1	68	contain	containing	107:116	arg1	compounds					97:105	Natural compounds	89:105	Natural compounds containing polysaccharide ingredients	89:143	Natural compounds containing polysaccharide ingredients have been employed as candidates for treatment of skin tissue.
28777137	1	68	contain	containing	107:116	arg1	candidates					167:176	candidates	167:176	candidates for treatment of skin tissue	167:205	Natural compounds containing polysaccharide ingredients have been employed as candidates for treatment of skin tissue.
28777137	1	68	contain	containing	107:116	arg2	ingredients					133:143	polysaccharide ingredients	118:143	polysaccharide ingredients	118:143	Natural compounds containing polysaccharide ingredients have been employed as candidates for treatment of skin tissue.
28777137	11	69	theme	similar	2073:2079	arg1	behavior					2064:2071	an elastic behavior	2053:2071	an elastic behavior similar to native skin tissue	2053:2101	The corresponding study tries to introduce a new type of natural-based scaffolds for dermal tissue engineering that exhibits an elastic behavior similar to native skin tissue.
28777137	5	70	theme	interwoven	946:955	arg1	mat					957:959	the interwoven mat	942:959	the interwoven mat of nanofibers	942:973	FTIR spectroscopy was endorsed the presence of B. vulgaris extract within the interwoven mat of nanofibers.
28777137	8	71	theme	corresponding	1554:1566	arg1	membrane					1590:1597	the corresponding composite electrospun membrane	1550:1597	the corresponding composite electrospun membrane	1550:1597	After cell seeding process of keratinocyte cells on composite and noncomposite scaffolds, SEM and 3[4,5-dimethylthiazoyl-2-yl]-2,5 diphenyltetrazolium bromide (MTT) assays approved higher number of attached cells onto the corresponding composite electrospun membrane.
28777137	4	72	theme	microscope	718:727	arg1	examinations					729:740	Scanning electron microscope examinations	700:740	Scanning electron microscope examinations	700:740	Scanning electron microscope examinations have approved the uniform and homogeneous structure of composite nanofibers containing nylon polymer and B. vulgaris extract.
28777137	9	73	theme	Epidermal	1600:1608	arg1	expression					1615:1624	Epidermal gene expression	1600:1624	Epidermal gene expression such as involucrin, cytokeratin 10, and cytokeratin 14	1600:1679	Epidermal gene expression such as involucrin, cytokeratin 10, and cytokeratin 14 was observed through real-time polymerase chain reaction (PCR) technique.
28777137	8	74	theme	electrospun	1578:1588	arg1	membrane					1590:1597	the corresponding composite electrospun membrane	1550:1597	the corresponding composite electrospun membrane	1550:1597	After cell seeding process of keratinocyte cells on composite and noncomposite scaffolds, SEM and 3[4,5-dimethylthiazoyl-2-yl]-2,5 diphenyltetrazolium bromide (MTT) assays approved higher number of attached cells onto the corresponding composite electrospun membrane.
28777137	8	75	theme	keratinocyte	1362:1373	arg1	cells					1375:1379	keratinocyte cells	1362:1379	keratinocyte cells	1362:1379	After cell seeding process of keratinocyte cells on composite and noncomposite scaffolds, SEM and 3[4,5-dimethylthiazoyl-2-yl]-2,5 diphenyltetrazolium bromide (MTT) assays approved higher number of attached cells onto the corresponding composite electrospun membrane.
28777137	0	76	theme	Nylon-Beta	68:77	arg1	Vulgaris					79:86	Nylon-Beta Vulgaris	68:86	Nylon-Beta Vulgaris	68:86	Natural Compounds for Skin Tissue Engineering by Electrospinning of Nylon-Beta Vulgaris.
28777137	3	77	dep	transform	623:631	arg1	infrared					633:640	infrared	633:640	transform infrared (FTIR) spectroscopy, mechanical assay, and contact angle	623:697	Both prepared scaffolds including noncomposite and composite types were studied by Scanning electron microscope (SEM), Fourier transform infrared (FTIR) spectroscopy, mechanical assay, and contact angle.
28777137	5	78	theme	vulgaris	918:925	arg1	extract					927:933	B. vulgaris extract	915:933	B. vulgaris extract	915:933	FTIR spectroscopy was endorsed the presence of B. vulgaris extract within the interwoven mat of nanofibers.
28777137	6	79	theme	desirable	1066:1074	arg1	similarity					1076:1085	the desirable similarity	1062:1085	the desirable similarity between corresponding scaffold and native skin tissue	1062:1139	Also, measurement of mechanical property with cell-laden composite scaffolds approved the desirable similarity between corresponding scaffold and native skin tissue.
28777137	7	80	theme	lower	1271:1275	arg1	angle					1285:1289	lower contact angle	1271:1289	lower contact angle	1271:1289	To our surprise, it was found that compared with nylon nanofibrous scaffold, composite sample containing B. vulgaris extract has lower contact angle indicating a higher hydrophilic surface.
28777137	11	81	theme	natural-based	1985:1997	arg1	scaffolds					1999:2007	natural-based scaffolds	1985:2007	natural-based scaffolds for dermal tissue engineering	1985:2037	The corresponding study tries to introduce a new type of natural-based scaffolds for dermal tissue engineering that exhibits an elastic behavior similar to native skin tissue.
28777137	4	82	theme	Scanning	700:707	arg1	examinations					729:740	Scanning electron microscope examinations	700:740	Scanning electron microscope examinations	700:740	Scanning electron microscope examinations have approved the uniform and homogeneous structure of composite nanofibers containing nylon polymer and B. vulgaris extract.
28777137	3	83	theme	electron	588:595	arg1	SEM					609:611	SEM	609:611	SEM	609:611	Both prepared scaffolds including noncomposite and composite types were studied by Scanning electron microscope (SEM), Fourier transform infrared (FTIR) spectroscopy, mechanical assay, and contact angle.
28777137	3	83	theme	electron	588:595	arg1	Fourier					615:621	Fourier	615:621	Fourier transform infrared (FTIR) spectroscopy, mechanical assay, and contact angle	615:697	Both prepared scaffolds including noncomposite and composite types were studied by Scanning electron microscope (SEM), Fourier transform infrared (FTIR) spectroscopy, mechanical assay, and contact angle.
28777137	3	83	theme	electron	588:595	arg1	microscope					597:606	Scanning electron microscope	579:606	Scanning electron microscope (SEM)	579:612	Both prepared scaffolds including noncomposite and composite types were studied by Scanning electron microscope (SEM), Fourier transform infrared (FTIR) spectroscopy, mechanical assay, and contact angle.
28777137	6	84	theme	composite	1033:1041	arg1	scaffolds					1043:1051	cell-laden composite scaffolds	1022:1051	cell-laden composite scaffolds	1022:1051	Also, measurement of mechanical property with cell-laden composite scaffolds approved the desirable similarity between corresponding scaffold and native skin tissue.
28777137	9	85	theme	gene	1610:1613	arg1	expression					1615:1624	Epidermal gene expression	1600:1624	Epidermal gene expression such as involucrin, cytokeratin 10, and cytokeratin 14	1600:1679	Epidermal gene expression such as involucrin, cytokeratin 10, and cytokeratin 14 was observed through real-time polymerase chain reaction (PCR) technique.
28777137	7	86	theme	B.	1247:1248	arg1	extract					1259:1265	B. vulgaris extract	1247:1265	B. vulgaris extract	1247:1265	To our surprise, it was found that compared with nylon nanofibrous scaffold, composite sample containing B. vulgaris extract has lower contact angle indicating a higher hydrophilic surface.
28777137	8	87	from	process	1351:1357	arg1	scaffolds					1411:1419	composite and noncomposite scaffolds	1384:1419	composite and noncomposite scaffolds	1384:1419	After cell seeding process of keratinocyte cells on composite and noncomposite scaffolds, SEM and 3[4,5-dimethylthiazoyl-2-yl]-2,5 diphenyltetrazolium bromide (MTT) assays approved higher number of attached cells onto the corresponding composite electrospun membrane.
28777137	4	88	theme	composite	797:805	arg1	nanofibers					807:816	composite nanofibers	797:816	composite nanofibers containing nylon polymer and B. vulgaris extract	797:865	Scanning electron microscope examinations have approved the uniform and homogeneous structure of composite nanofibers containing nylon polymer and B. vulgaris extract.
28777137	1	89	theme	Natural	89:95	arg1	compounds					97:105	Natural compounds	89:105	Natural compounds containing polysaccharide ingredients	89:143	Natural compounds containing polysaccharide ingredients have been employed as candidates for treatment of skin tissue.
28777137	1	89	theme	Natural	89:95	arg1	candidates					167:176	candidates	167:176	candidates for treatment of skin tissue	167:205	Natural compounds containing polysaccharide ingredients have been employed as candidates for treatment of skin tissue.
28777137	8	90	theme	3[4,5-dimethylthiazoyl-2-yl	1430:1456	arg1	MTT					1492:1494	MTT	1492:1494	MTT	1492:1494	After cell seeding process of keratinocyte cells on composite and noncomposite scaffolds, SEM and 3[4,5-dimethylthiazoyl-2-yl]-2,5 diphenyltetrazolium bromide (MTT) assays approved higher number of attached cells onto the corresponding composite electrospun membrane.
28777137	8	90	theme	3[4,5-dimethylthiazoyl-2-yl	1430:1456	arg1	bromide					1483:1489	SEM and 3[4,5-dimethylthiazoyl-2-yl]-2,5 diphenyltetrazolium bromide	1422:1489	bromide	1483:1489	After cell seeding process of keratinocyte cells on composite and noncomposite scaffolds, SEM and 3[4,5-dimethylthiazoyl-2-yl]-2,5 diphenyltetrazolium bromide (MTT) assays approved higher number of attached cells onto the corresponding composite electrospun membrane.
28777137	11	91	theme	tissue	2020:2025	arg1	engineering					2027:2037	dermal tissue engineering	2013:2037	dermal tissue engineering	2013:2037	The corresponding study tries to introduce a new type of natural-based scaffolds for dermal tissue engineering that exhibits an elastic behavior similar to native skin tissue.
28777137	0	92	theme	Skin	22:25	arg1	Engineering					34:44	Skin Tissue Engineering	22:44	Skin Tissue Engineering by Electrospinning of Nylon-Beta Vulgaris	22:86	Natural Compounds for Skin Tissue Engineering by Electrospinning of Nylon-Beta Vulgaris.
28777137	11	93	theme	native	2084:2089	arg1	tissue					2096:2101	native skin tissue	2084:2101	native skin tissue	2084:2101	The corresponding study tries to introduce a new type of natural-based scaffolds for dermal tissue engineering that exhibits an elastic behavior similar to native skin tissue.
28777137	6	94	theme	native	1122:1127	arg1	tissue					1134:1139	native skin tissue	1122:1139	native skin tissue	1122:1139	Also, measurement of mechanical property with cell-laden composite scaffolds approved the desirable similarity between corresponding scaffold and native skin tissue.
28777137	8	95	theme	-2,5	1458:1461	arg1	MTT					1492:1494	MTT	1492:1494	MTT	1492:1494	After cell seeding process of keratinocyte cells on composite and noncomposite scaffolds, SEM and 3[4,5-dimethylthiazoyl-2-yl]-2,5 diphenyltetrazolium bromide (MTT) assays approved higher number of attached cells onto the corresponding composite electrospun membrane.
28777137	8	95	theme	-2,5	1458:1461	arg1	bromide					1483:1489	SEM and 3[4,5-dimethylthiazoyl-2-yl]-2,5 diphenyltetrazolium bromide	1422:1489	bromide	1483:1489	After cell seeding process of keratinocyte cells on composite and noncomposite scaffolds, SEM and 3[4,5-dimethylthiazoyl-2-yl]-2,5 diphenyltetrazolium bromide (MTT) assays approved higher number of attached cells onto the corresponding composite electrospun membrane.
28777137	7	96	theme	nanofibrous	1197:1207	arg1	scaffold					1209:1216	nylon nanofibrous scaffold	1191:1216	nylon nanofibrous scaffold	1191:1216	To our surprise, it was found that compared with nylon nanofibrous scaffold, composite sample containing B. vulgaris extract has lower contact angle indicating a higher hydrophilic surface.
28777137	2	97	theme	tissue	476:481	arg1	engineering					483:493	skin tissue engineering	471:493	skin tissue engineering	471:493	Herein, for the first time, electrospinning setup was proposed to fabricate an efficient composite nanofibrous structure of Beta vulgaris (obtained from Beet [Chenopodiaceae or Amaranthaceae]) belonged to polysaccharides and an elastic polymer named nylon 66 for skin tissue engineering.
28777137	4	98	theme	B.	847:848	arg1	extract					859:865	B. vulgaris extract	847:865	B. vulgaris extract	847:865	Scanning electron microscope examinations have approved the uniform and homogeneous structure of composite nanofibers containing nylon polymer and B. vulgaris extract.
28777137	9	99	theme	real-time	1702:1710	arg1	reaction					1729:1736	real-time polymerase chain reaction	1702:1736	real-time polymerase chain reaction (PCR) technique	1702:1752	Epidermal gene expression such as involucrin, cytokeratin 10, and cytokeratin 14 was observed through real-time polymerase chain reaction (PCR) technique.
28777137	9	99	theme	real-time	1702:1710	arg1	PCR					1739:1741	PCR	1739:1741	PCR	1739:1741	Epidermal gene expression such as involucrin, cytokeratin 10, and cytokeratin 14 was observed through real-time polymerase chain reaction (PCR) technique.
28777137	7	100	contain	has	1267:1269	arg2	angle					1285:1289	lower contact angle	1271:1289	lower contact angle	1271:1289	To our surprise, it was found that compared with nylon nanofibrous scaffold, composite sample containing B. vulgaris extract has lower contact angle indicating a higher hydrophilic surface.
28777137	7	100	contain	has	1267:1269	arg1	sample					1229:1234	composite sample	1219:1234	composite sample containing B. vulgaris extract	1219:1265	To our surprise, it was found that compared with nylon nanofibrous scaffold, composite sample containing B. vulgaris extract has lower contact angle indicating a higher hydrophilic surface.
28777137	9	101	theme	chain	1723:1727	arg1	reaction					1729:1736	real-time polymerase chain reaction	1702:1736	real-time polymerase chain reaction (PCR) technique	1702:1752	Epidermal gene expression such as involucrin, cytokeratin 10, and cytokeratin 14 was observed through real-time polymerase chain reaction (PCR) technique.
28777137	9	101	theme	chain	1723:1727	arg1	PCR					1739:1741	PCR	1739:1741	PCR	1739:1741	Epidermal gene expression such as involucrin, cytokeratin 10, and cytokeratin 14 was observed through real-time polymerase chain reaction (PCR) technique.
28777137	8	102	theme	SEM	1422:1424	arg1	MTT					1492:1494	MTT	1492:1494	MTT	1492:1494	After cell seeding process of keratinocyte cells on composite and noncomposite scaffolds, SEM and 3[4,5-dimethylthiazoyl-2-yl]-2,5 diphenyltetrazolium bromide (MTT) assays approved higher number of attached cells onto the corresponding composite electrospun membrane.
28777137	8	102	theme	SEM	1422:1424	arg1	bromide					1483:1489	SEM and 3[4,5-dimethylthiazoyl-2-yl]-2,5 diphenyltetrazolium bromide	1422:1489	bromide	1483:1489	After cell seeding process of keratinocyte cells on composite and noncomposite scaffolds, SEM and 3[4,5-dimethylthiazoyl-2-yl]-2,5 diphenyltetrazolium bromide (MTT) assays approved higher number of attached cells onto the corresponding composite electrospun membrane.
28777137	2	103	theme	vulgaris	337:344	arg1	structure					319:327	an efficient composite nanofibrous structure	284:327	an efficient composite nanofibrous structure of Beta vulgaris (obtained from Beet [Chenopodiaceae or Amaranthaceae]) belonged to polysaccharides and an elastic polymer named nylon 66	284:465	Herein, for the first time, electrospinning setup was proposed to fabricate an efficient composite nanofibrous structure of Beta vulgaris (obtained from Beet [Chenopodiaceae or Amaranthaceae]) belonged to polysaccharides and an elastic polymer named nylon 66 for skin tissue engineering.
28777137	7	104	theme	hydrophilic	1311:1321	arg1	surface					1323:1329	a higher hydrophilic surface	1302:1329	a higher hydrophilic surface	1302:1329	To our surprise, it was found that compared with nylon nanofibrous scaffold, composite sample containing B. vulgaris extract has lower contact angle indicating a higher hydrophilic surface.
28777137	8	105	theme	higher	1513:1518	arg1	number					1520:1525	higher number	1513:1525	higher number of attached cells onto the corresponding composite electrospun membrane	1513:1597	After cell seeding process of keratinocyte cells on composite and noncomposite scaffolds, SEM and 3[4,5-dimethylthiazoyl-2-yl]-2,5 diphenyltetrazolium bromide (MTT) assays approved higher number of attached cells onto the corresponding composite electrospun membrane.
28777137	3	106	theme	composite	547:555	arg1	types					557:561	composite types	547:561	composite types	547:561	Both prepared scaffolds including noncomposite and composite types were studied by Scanning electron microscope (SEM), Fourier transform infrared (FTIR) spectroscopy, mechanical assay, and contact angle.
30721733	6	0	theme	gene	901:904	arg1	expression					906:915	gene expression	901:915	gene expression	901:915	After 72 h in culture, depth-dependent NP cell viability and gene expression were measured, and related to CEP transport properties and biochemical composition determined using fluorescence recovery after photobleaching and Fourier transform infrared (FTIR) spectroscopy.
30721733	7	1	theme	lower	1246:1250	arg1	expression					1310:1319	lower aggrecan, collagen-2, and matrix metalloproteinase-2 gene expression	1246:1319	lower aggrecan, collagen-2, and matrix metalloproteinase-2 gene expression	1246:1319	RESULTS Solute diffusivity varied nearly 4-fold amongst the CEPs studied, and chambers with the least permeable CEPs appeared to have lower aggrecan, collagen-2, and matrix metalloproteinase-2 gene expression, as well as a significantly shorter viable distance from the CEP/nutrient interface.
30721733	11	2	theme	nutrient	2004:2011	arg1	supply					2013:2018	increased nutrient supply	1994:2018	increased nutrient supply	1994:2018	Degeneration-related CEP matrix changes could hinder the success of biologic therapies that require increased nutrient supply.
30721733	4	3	theme	fresh	600:604	arg1	spines					623:628	six fresh cadaveric lumbar spines	596:628	six fresh cadaveric lumbar spines (38-66 years old)	596:646	DESIGN Human CEP tissues harvested from six fresh cadaveric lumbar spines (38-66 years old) were placed at the open sides of diffusion chambers.
30721733	6	4	theme	NP	879:880	arg1	viability					887:895	depth-dependent NP cell viability	863:895	depth-dependent NP cell viability	863:895	After 72 h in culture, depth-dependent NP cell viability and gene expression were measured, and related to CEP transport properties and biochemical composition determined using fluorescence recovery after photobleaching and Fourier transform infrared (FTIR) spectroscopy.
30721733	7	5	theme	RESULTS	1112:1118	arg1	diffusivity					1127:1137	RESULTS Solute diffusivity	1112:1137	RESULTS Solute diffusivity	1112:1137	RESULTS Solute diffusivity varied nearly 4-fold amongst the CEPs studied, and chambers with the least permeable CEPs appeared to have lower aggrecan, collagen-2, and matrix metalloproteinase-2 gene expression, as well as a significantly shorter viable distance from the CEP/nutrient interface.
30721733	1	6	theme	OBJECTIVE	175:183	arg1	strategy					229:236	a promising strategy	217:236	a promising strategy for managing intervertebral disc degeneration	217:282	OBJECTIVE Intradiscal biologic therapy is a promising strategy for managing intervertebral disc degeneration.
30721733	1	6	theme	OBJECTIVE	175:183	arg1	therapy					206:212	OBJECTIVE Intradiscal biologic therapy	175:212	OBJECTIVE Intradiscal biologic therapy	175:212	OBJECTIVE Intradiscal biologic therapy is a promising strategy for managing intervertebral disc degeneration.
30721733	9	7	theme	low-diffusivity	1698:1712	arg1	CEPs					1714:1717	low-diffusivity CEPs	1698:1717	low-diffusivity CEPs	1698:1717	Solute diffusivity in the CEP was associated with biochemical composition: low-diffusivity CEPs had greater amounts of collagen and aggrecan, more mineral, and lower cross-link maturity.
30721733	2	8	theme	nutrient	325:332	arg1	supply					334:339	a rich nutrient supply	318:339	a rich nutrient supply	318:339	However, these therapies require a rich nutrient supply, which may be limited by the transport properties of the cartilage endplate (CEP).
30721733	1	9	theme	biologic	197:204	arg1	strategy					229:236	a promising strategy	217:236	a promising strategy for managing intervertebral disc degeneration	217:282	OBJECTIVE Intradiscal biologic therapy is a promising strategy for managing intervertebral disc degeneration.
30721733	1	9	theme	biologic	197:204	arg1	therapy					206:212	OBJECTIVE Intradiscal biologic therapy	175:212	OBJECTIVE Intradiscal biologic therapy	175:212	OBJECTIVE Intradiscal biologic therapy is a promising strategy for managing intervertebral disc degeneration.
30721733	6	10	theme	CEP	947:949	arg1	properties					961:970	CEP transport properties	947:970	CEP transport properties	947:970	After 72 h in culture, depth-dependent NP cell viability and gene expression were measured, and related to CEP transport properties and biochemical composition determined using fluorescence recovery after photobleaching and Fourier transform infrared (FTIR) spectroscopy.
30721733	0	11	theme	endplate	105:112	arg1	properties					77:86	the transport properties	63:86	the transport properties of the cartilage endplate	63:112	Nutrient supply and nucleus pulposus cell function: effects of the transport properties of the cartilage endplate and potential implications for intradiscal biologic therapy.
30721733	5	12	theme	CEP	827:829	arg1	tissues					831:837	the CEP tissues	823:837	the CEP tissues	823:837	Bovine nucleus pulposus (NP) cells cultured inside the chambers were nourished exclusively by nutrients diffusing through the CEP tissues.
30721733	6	13	theme	related	936:942	arg1	composition					988:998	related to CEP transport properties and biochemical composition	936:998	related to CEP transport properties and biochemical composition	936:998	After 72 h in culture, depth-dependent NP cell viability and gene expression were measured, and related to CEP transport properties and biochemical composition determined using fluorescence recovery after photobleaching and Fourier transform infrared (FTIR) spectroscopy.
30721733	0	14	theme	potential	118:126	arg1	implications					128:139	potential implications	118:139	potential implications for intradiscal biologic therapy	118:172	Nutrient supply and nucleus pulposus cell function: effects of the transport properties of the cartilage endplate and potential implications for intradiscal biologic therapy.
30721733	10	15	theme	transport	1826:1834	arg1	properties					1836:1845	CONCLUSIONS CEP transport properties	1810:1845	CONCLUSIONS CEP transport properties	1810:1845	CONCLUSIONS CEP transport properties dramatically affect NP cell survival/function.
30721733	1	16	theme	promising	219:227	arg1	strategy					229:236	a promising strategy	217:236	a promising strategy for managing intervertebral disc degeneration	217:282	OBJECTIVE Intradiscal biologic therapy is a promising strategy for managing intervertebral disc degeneration.
30721733	1	16	theme	promising	219:227	arg1	therapy					206:212	OBJECTIVE Intradiscal biologic therapy	175:212	OBJECTIVE Intradiscal biologic therapy	175:212	OBJECTIVE Intradiscal biologic therapy is a promising strategy for managing intervertebral disc degeneration.
30721733	9	17	theme	collagen	1742:1749	arg1	amounts					1731:1737	greater amounts	1723:1737	greater amounts of collagen and aggrecan	1723:1762	Solute diffusivity in the CEP was associated with biochemical composition: low-diffusivity CEPs had greater amounts of collagen and aggrecan, more mineral, and lower cross-link maturity.
30721733	9	17	theme	collagen	1742:1749	arg1	aggrecan					1755:1762	aggrecan	1755:1762	aggrecan	1755:1762	Solute diffusivity in the CEP was associated with biochemical composition: low-diffusivity CEPs had greater amounts of collagen and aggrecan, more mineral, and lower cross-link maturity.
30721733	9	17	theme	collagen	1742:1749	arg1	maturity					1800:1807	lower cross-link maturity	1783:1807	lower cross-link maturity	1783:1807	Solute diffusivity in the CEP was associated with biochemical composition: low-diffusivity CEPs had greater amounts of collagen and aggrecan, more mineral, and lower cross-link maturity.
30721733	9	17	theme	collagen	1742:1749	arg1	collagen					1742:1749	collagen	1742:1749	collagen	1742:1749	Solute diffusivity in the CEP was associated with biochemical composition: low-diffusivity CEPs had greater amounts of collagen and aggrecan, more mineral, and lower cross-link maturity.
30721733	10	18	theme	CONCLUSIONS	1810:1820	arg1	properties					1836:1845	CONCLUSIONS CEP transport properties	1810:1845	CONCLUSIONS CEP transport properties	1810:1845	CONCLUSIONS CEP transport properties dramatically affect NP cell survival/function.
30721733	9	19	theme	aggrecan	1755:1762	arg1	amounts					1731:1737	greater amounts	1723:1737	greater amounts of collagen and aggrecan	1723:1762	Solute diffusivity in the CEP was associated with biochemical composition: low-diffusivity CEPs had greater amounts of collagen and aggrecan, more mineral, and lower cross-link maturity.
30721733	9	19	theme	aggrecan	1755:1762	arg1	aggrecan					1755:1762	aggrecan	1755:1762	aggrecan	1755:1762	Solute diffusivity in the CEP was associated with biochemical composition: low-diffusivity CEPs had greater amounts of collagen and aggrecan, more mineral, and lower cross-link maturity.
30721733	9	19	theme	aggrecan	1755:1762	arg1	maturity					1800:1807	lower cross-link maturity	1783:1807	lower cross-link maturity	1783:1807	Solute diffusivity in the CEP was associated with biochemical composition: low-diffusivity CEPs had greater amounts of collagen and aggrecan, more mineral, and lower cross-link maturity.
30721733	9	19	theme	aggrecan	1755:1762	arg1	collagen					1742:1749	collagen	1742:1749	collagen	1742:1749	Solute diffusivity in the CEP was associated with biochemical composition: low-diffusivity CEPs had greater amounts of collagen and aggrecan, more mineral, and lower cross-link maturity.
30721733	0	20	theme	biologic	157:164	arg1	therapy					166:172	intradiscal biologic therapy	145:172	intradiscal biologic therapy	145:172	Nutrient supply and nucleus pulposus cell function: effects of the transport properties of the cartilage endplate and potential implications for intradiscal biologic therapy.
30721733	10	21	theme	NP	1867:1868	arg1	survival/function					1875:1891	NP cell survival/function	1867:1891	NP cell survival/function	1867:1891	CONCLUSIONS CEP transport properties dramatically affect NP cell survival/function.
30721733	6	22	theme	fluorescence	1017:1028	arg1	recovery					1030:1037	fluorescence recovery	1017:1037	fluorescence recovery	1017:1037	After 72 h in culture, depth-dependent NP cell viability and gene expression were measured, and related to CEP transport properties and biochemical composition determined using fluorescence recovery after photobleaching and Fourier transform infrared (FTIR) spectroscopy.
30721733	7	23	theme	shorter	1349:1355	arg1	distance					1364:1371	a significantly shorter viable distance	1333:1371	a significantly shorter viable distance from the CEP/nutrient interface	1333:1403	RESULTS Solute diffusivity varied nearly 4-fold amongst the CEPs studied, and chambers with the least permeable CEPs appeared to have lower aggrecan, collagen-2, and matrix metalloproteinase-2 gene expression, as well as a significantly shorter viable distance from the CEP/nutrient interface.
30721733	8	24	theme	low-diffusivity	1503:1517	arg1	CEPs					1519:1522	low-diffusivity CEPs	1503:1522	low-diffusivity CEPs	1503:1522	Increasing chamber cell density shortened the viable distance; however, this effect was lost for low-diffusivity CEPs, which suggests that these CEPs may not provide enough nutrient diffusion to satisfy cell demands.
30721733	4	25	theme	diffusion	681:689	arg1	chambers					691:698	diffusion chambers	681:698	diffusion chambers	681:698	DESIGN Human CEP tissues harvested from six fresh cadaveric lumbar spines (38-66 years old) were placed at the open sides of diffusion chambers.
30721733	6	26	theme	biochemical	976:986	arg1	composition					988:998	related to CEP transport properties and biochemical composition	936:998	related to CEP transport properties and biochemical composition	936:998	After 72 h in culture, depth-dependent NP cell viability and gene expression were measured, and related to CEP transport properties and biochemical composition determined using fluorescence recovery after photobleaching and Fourier transform infrared (FTIR) spectroscopy.
30721733	9	27	theme	lower	1783:1787	arg1	maturity					1800:1807	lower cross-link maturity	1783:1807	lower cross-link maturity	1783:1807	Solute diffusivity in the CEP was associated with biochemical composition: low-diffusivity CEPs had greater amounts of collagen and aggrecan, more mineral, and lower cross-link maturity.
30721733	0	28	theme	properties	77:86	arg1	effects					52:58	effects	52:58	effects of the transport properties of the cartilage endplate	52:112	Nutrient supply and nucleus pulposus cell function: effects of the transport properties of the cartilage endplate and potential implications for intradiscal biologic therapy.
30721733	0	28	theme	properties	77:86	arg1	implications					128:139	potential implications	118:139	potential implications for intradiscal biologic therapy	118:172	Nutrient supply and nucleus pulposus cell function: effects of the transport properties of the cartilage endplate and potential implications for intradiscal biologic therapy.
30721733	11	29	theme	increased	1994:2002	arg1	supply					2013:2018	increased nutrient supply	1994:2018	increased nutrient supply	1994:2018	Degeneration-related CEP matrix changes could hinder the success of biologic therapies that require increased nutrient supply.
30721733	6	30	dep	Fourier	1064:1070	arg1	transform					1072:1080	transform	1072:1080	transform infrared (FTIR) spectroscopy	1072:1109	After 72 h in culture, depth-dependent NP cell viability and gene expression were measured, and related to CEP transport properties and biochemical composition determined using fluorescence recovery after photobleaching and Fourier transform infrared (FTIR) spectroscopy.
30721733	2	31	theme	cartilage	398:406	arg1	CEP					418:420	CEP	418:420	CEP	418:420	However, these therapies require a rich nutrient supply, which may be limited by the transport properties of the cartilage endplate (CEP).
30721733	2	31	theme	cartilage	398:406	arg1	endplate					408:415	the cartilage endplate	394:415	the cartilage endplate (CEP)	394:421	However, these therapies require a rich nutrient supply, which may be limited by the transport properties of the cartilage endplate (CEP).
30721733	9	32	from	diffusivity	1630:1640	arg1	CEP					1649:1651	the CEP	1645:1651	the CEP	1645:1651	Solute diffusivity in the CEP was associated with biochemical composition: low-diffusivity CEPs had greater amounts of collagen and aggrecan, more mineral, and lower cross-link maturity.
30721733	6	33	dep	infrared	1082:1089	arg1	FTIR					1092:1095	FTIR	1092:1095	FTIR	1092:1095	After 72 h in culture, depth-dependent NP cell viability and gene expression were measured, and related to CEP transport properties and biochemical composition determined using fluorescence recovery after photobleaching and Fourier transform infrared (FTIR) spectroscopy.
30721733	0	34	theme	Nutrient	0:7	arg1	supply					9:14	Nutrient supply	0:14	Nutrient supply	0:14	Nutrient supply and nucleus pulposus cell function: effects of the transport properties of the cartilage endplate and potential implications for intradiscal biologic therapy.
30721733	4	35	theme	DESIGN	556:561	arg1	tissues					573:579	DESIGN Human CEP tissues	556:579	DESIGN Human CEP tissues harvested from six fresh cadaveric lumbar spines (38-66 years old)	556:646	DESIGN Human CEP tissues harvested from six fresh cadaveric lumbar spines (38-66 years old) were placed at the open sides of diffusion chambers.
30721733	3	36	dep	cell	528:531	arg1	survival					533:540	survival	533:540	survival	533:540	This study investigated how fluctuations in CEP transport properties impact nutrient diffusion and disc cell survival and function.
30721733	7	37	from	interface	1395:1403	arg1	distance					1364:1371	a significantly shorter viable distance	1333:1371	a significantly shorter viable distance from the CEP/nutrient interface	1333:1403	RESULTS Solute diffusivity varied nearly 4-fold amongst the CEPs studied, and chambers with the least permeable CEPs appeared to have lower aggrecan, collagen-2, and matrix metalloproteinase-2 gene expression, as well as a significantly shorter viable distance from the CEP/nutrient interface.
30721733	7	37	from	interface	1395:1403	arg1	expression					1310:1319	lower aggrecan, collagen-2, and matrix metalloproteinase-2 gene expression	1246:1319	lower aggrecan, collagen-2, and matrix metalloproteinase-2 gene expression	1246:1319	RESULTS Solute diffusivity varied nearly 4-fold amongst the CEPs studied, and chambers with the least permeable CEPs appeared to have lower aggrecan, collagen-2, and matrix metalloproteinase-2 gene expression, as well as a significantly shorter viable distance from the CEP/nutrient interface.
30721733	4	38	theme	CEP	569:571	arg1	tissues					573:579	DESIGN Human CEP tissues	556:579	DESIGN Human CEP tissues harvested from six fresh cadaveric lumbar spines (38-66 years old)	556:646	DESIGN Human CEP tissues harvested from six fresh cadaveric lumbar spines (38-66 years old) were placed at the open sides of diffusion chambers.
30721733	8	39	theme	chamber	1417:1423	arg1	density					1430:1436	Increasing chamber cell density	1406:1436	Increasing chamber cell density	1406:1436	Increasing chamber cell density shortened the viable distance; however, this effect was lost for low-diffusivity CEPs, which suggests that these CEPs may not provide enough nutrient diffusion to satisfy cell demands.
30721733	11	40	theme	therapies	1971:1979	arg1	success					1951:1957	the success	1947:1957	the success of biologic therapies that require increased nutrient supply	1947:2018	Degeneration-related CEP matrix changes could hinder the success of biologic therapies that require increased nutrient supply.
30721733	0	41	theme	pulposus	28:35	arg1	function					42:49	nucleus pulposus cell function	20:49	nucleus pulposus cell function	20:49	Nutrient supply and nucleus pulposus cell function: effects of the transport properties of the cartilage endplate and potential implications for intradiscal biologic therapy.
30721733	8	42	theme	nutrient	1579:1586	arg1	diffusion					1588:1596	nutrient diffusion	1579:1596	nutrient diffusion to satisfy cell demands	1579:1620	Increasing chamber cell density shortened the viable distance; however, this effect was lost for low-diffusivity CEPs, which suggests that these CEPs may not provide enough nutrient diffusion to satisfy cell demands.
30721733	3	43	from	fluctuations	452:463	arg1	properties					482:491	CEP transport properties	468:491	CEP transport properties	468:491	This study investigated how fluctuations in CEP transport properties impact nutrient diffusion and disc cell survival and function.
30721733	11	44	theme	Degeneration-related	1894:1913	arg1	changes					1926:1932	Degeneration-related CEP matrix changes	1894:1932	Degeneration-related CEP matrix changes	1894:1932	Degeneration-related CEP matrix changes could hinder the success of biologic therapies that require increased nutrient supply.
30721733	9	45	theme	Solute	1623:1628	arg1	diffusivity					1630:1640	Solute diffusivity	1623:1640	Solute diffusivity in the CEP	1623:1651	Solute diffusivity in the CEP was associated with biochemical composition: low-diffusivity CEPs had greater amounts of collagen and aggrecan, more mineral, and lower cross-link maturity.
30721733	7	46	theme	metalloproteinase-2	1285:1303	arg1	expression					1310:1319	lower aggrecan, collagen-2, and matrix metalloproteinase-2 gene expression	1246:1319	lower aggrecan, collagen-2, and matrix metalloproteinase-2 gene expression	1246:1319	RESULTS Solute diffusivity varied nearly 4-fold amongst the CEPs studied, and chambers with the least permeable CEPs appeared to have lower aggrecan, collagen-2, and matrix metalloproteinase-2 gene expression, as well as a significantly shorter viable distance from the CEP/nutrient interface.
30721733	3	47	theme	transport	472:480	arg1	properties					482:491	CEP transport properties	468:491	CEP transport properties	468:491	This study investigated how fluctuations in CEP transport properties impact nutrient diffusion and disc cell survival and function.
30721733	8	48	theme	cell	1609:1612	arg1	demands					1614:1620	cell demands	1609:1620	cell demands	1609:1620	Increasing chamber cell density shortened the viable distance; however, this effect was lost for low-diffusivity CEPs, which suggests that these CEPs may not provide enough nutrient diffusion to satisfy cell demands.
30721733	3	49	theme	disc	523:526	arg1	cell					528:531	disc cell survival and function	523:553	cell	528:531	This study investigated how fluctuations in CEP transport properties impact nutrient diffusion and disc cell survival and function.
30721733	7	50	theme	collagen-2	1262:1271	arg1	expression					1310:1319	lower aggrecan, collagen-2, and matrix metalloproteinase-2 gene expression	1246:1319	lower aggrecan, collagen-2, and matrix metalloproteinase-2 gene expression	1246:1319	RESULTS Solute diffusivity varied nearly 4-fold amongst the CEPs studied, and chambers with the least permeable CEPs appeared to have lower aggrecan, collagen-2, and matrix metalloproteinase-2 gene expression, as well as a significantly shorter viable distance from the CEP/nutrient interface.
30721733	11	51	theme	matrix	1919:1924	arg1	changes					1926:1932	Degeneration-related CEP matrix changes	1894:1932	Degeneration-related CEP matrix changes	1894:1932	Degeneration-related CEP matrix changes could hinder the success of biologic therapies that require increased nutrient supply.
30721733	1	52	theme	disc	266:269	arg1	degeneration					271:282	intervertebral disc degeneration	251:282	intervertebral disc degeneration	251:282	OBJECTIVE Intradiscal biologic therapy is a promising strategy for managing intervertebral disc degeneration.
30721733	7	53	theme	aggrecan	1252:1259	arg1	expression					1310:1319	lower aggrecan, collagen-2, and matrix metalloproteinase-2 gene expression	1246:1319	lower aggrecan, collagen-2, and matrix metalloproteinase-2 gene expression	1246:1319	RESULTS Solute diffusivity varied nearly 4-fold amongst the CEPs studied, and chambers with the least permeable CEPs appeared to have lower aggrecan, collagen-2, and matrix metalloproteinase-2 gene expression, as well as a significantly shorter viable distance from the CEP/nutrient interface.
30721733	7	54	theme	Solute	1120:1125	arg1	diffusivity					1127:1137	RESULTS Solute diffusivity	1112:1137	RESULTS Solute diffusivity	1112:1137	RESULTS Solute diffusivity varied nearly 4-fold amongst the CEPs studied, and chambers with the least permeable CEPs appeared to have lower aggrecan, collagen-2, and matrix metalloproteinase-2 gene expression, as well as a significantly shorter viable distance from the CEP/nutrient interface.
30721733	6	55	theme	cell	882:885	arg1	viability					887:895	depth-dependent NP cell viability	863:895	depth-dependent NP cell viability	863:895	After 72 h in culture, depth-dependent NP cell viability and gene expression were measured, and related to CEP transport properties and biochemical composition determined using fluorescence recovery after photobleaching and Fourier transform infrared (FTIR) spectroscopy.
30721733	9	56	theme	biochemical	1673:1683	arg1	composition					1685:1695	biochemical composition	1673:1695	biochemical composition	1673:1695	Solute diffusivity in the CEP was associated with biochemical composition: low-diffusivity CEPs had greater amounts of collagen and aggrecan, more mineral, and lower cross-link maturity.
30721733	6	57	theme	depth-dependent	863:877	arg1	viability					887:895	depth-dependent NP cell viability	863:895	depth-dependent NP cell viability	863:895	After 72 h in culture, depth-dependent NP cell viability and gene expression were measured, and related to CEP transport properties and biochemical composition determined using fluorescence recovery after photobleaching and Fourier transform infrared (FTIR) spectroscopy.
30721733	6	58	from	72 h	846:849	arg1	culture					854:860	culture	854:860	culture	854:860	After 72 h in culture, depth-dependent NP cell viability and gene expression were measured, and related to CEP transport properties and biochemical composition determined using fluorescence recovery after photobleaching and Fourier transform infrared (FTIR) spectroscopy.
30721733	1	59	theme	Intradiscal	185:195	arg1	strategy					229:236	a promising strategy	217:236	a promising strategy for managing intervertebral disc degeneration	217:282	OBJECTIVE Intradiscal biologic therapy is a promising strategy for managing intervertebral disc degeneration.
30721733	1	59	theme	Intradiscal	185:195	arg1	therapy					206:212	OBJECTIVE Intradiscal biologic therapy	175:212	OBJECTIVE Intradiscal biologic therapy	175:212	OBJECTIVE Intradiscal biologic therapy is a promising strategy for managing intervertebral disc degeneration.
30721733	6	60	theme	transport	951:959	arg1	properties					961:970	CEP transport properties	947:970	CEP transport properties	947:970	After 72 h in culture, depth-dependent NP cell viability and gene expression were measured, and related to CEP transport properties and biochemical composition determined using fluorescence recovery after photobleaching and Fourier transform infrared (FTIR) spectroscopy.
30721733	2	61	theme	rich	320:323	arg1	supply					334:339	a rich nutrient supply	318:339	a rich nutrient supply	318:339	However, these therapies require a rich nutrient supply, which may be limited by the transport properties of the cartilage endplate (CEP).
30721733	9	62	theme	greater	1723:1729	arg1	amounts					1731:1737	greater amounts	1723:1737	greater amounts of collagen and aggrecan	1723:1762	Solute diffusivity in the CEP was associated with biochemical composition: low-diffusivity CEPs had greater amounts of collagen and aggrecan, more mineral, and lower cross-link maturity.
30721733	9	62	theme	greater	1723:1729	arg1	aggrecan					1755:1762	aggrecan	1755:1762	aggrecan	1755:1762	Solute diffusivity in the CEP was associated with biochemical composition: low-diffusivity CEPs had greater amounts of collagen and aggrecan, more mineral, and lower cross-link maturity.
30721733	9	62	theme	greater	1723:1729	arg1	collagen					1742:1749	collagen	1742:1749	collagen	1742:1749	Solute diffusivity in the CEP was associated with biochemical composition: low-diffusivity CEPs had greater amounts of collagen and aggrecan, more mineral, and lower cross-link maturity.
30721733	7	63	with	chambers	1190:1197	arg1	CEPs					1224:1227	the least permeable CEPs	1204:1227	the least permeable CEPs	1204:1227	RESULTS Solute diffusivity varied nearly 4-fold amongst the CEPs studied, and chambers with the least permeable CEPs appeared to have lower aggrecan, collagen-2, and matrix metalloproteinase-2 gene expression, as well as a significantly shorter viable distance from the CEP/nutrient interface.
30721733	11	64	theme	CEP	1915:1917	arg1	changes					1926:1932	Degeneration-related CEP matrix changes	1894:1932	Degeneration-related CEP matrix changes	1894:1932	Degeneration-related CEP matrix changes could hinder the success of biologic therapies that require increased nutrient supply.
30721733	5	65	theme	Bovine	701:706	arg1	NP					726:727	NP	726:727	NP	726:727	Bovine nucleus pulposus (NP) cells cultured inside the chambers were nourished exclusively by nutrients diffusing through the CEP tissues.
30721733	5	65	theme	Bovine	701:706	arg1	pulposus					716:723	Bovine nucleus pulposus	701:723	Bovine nucleus pulposus (NP) cells cultured inside the chambers	701:763	Bovine nucleus pulposus (NP) cells cultured inside the chambers were nourished exclusively by nutrients diffusing through the CEP tissues.
30721733	0	66	theme	intradiscal	145:155	arg1	therapy					166:172	intradiscal biologic therapy	145:172	intradiscal biologic therapy	145:172	Nutrient supply and nucleus pulposus cell function: effects of the transport properties of the cartilage endplate and potential implications for intradiscal biologic therapy.
30721733	10	67	theme	CEP	1822:1824	arg1	properties					1836:1845	CONCLUSIONS CEP transport properties	1810:1845	CONCLUSIONS CEP transport properties	1810:1845	CONCLUSIONS CEP transport properties dramatically affect NP cell survival/function.
30721733	7	68	theme	CEP/nutrient	1382:1393	arg1	interface					1395:1403	the CEP/nutrient interface	1378:1403	the CEP/nutrient interface	1378:1403	RESULTS Solute diffusivity varied nearly 4-fold amongst the CEPs studied, and chambers with the least permeable CEPs appeared to have lower aggrecan, collagen-2, and matrix metalloproteinase-2 gene expression, as well as a significantly shorter viable distance from the CEP/nutrient interface.
30721733	4	69	theme	chambers	691:698	arg1	sides					672:676	the open sides	663:676	the open sides of diffusion chambers	663:698	DESIGN Human CEP tissues harvested from six fresh cadaveric lumbar spines (38-66 years old) were placed at the open sides of diffusion chambers.
30721733	4	70	dep	spines	623:628	arg1	old					643:645	old	643:645	old	643:645	DESIGN Human CEP tissues harvested from six fresh cadaveric lumbar spines (38-66 years old) were placed at the open sides of diffusion chambers.
30721733	10	71	theme	cell	1870:1873	arg1	survival/function					1875:1891	NP cell survival/function	1867:1891	NP cell survival/function	1867:1891	CONCLUSIONS CEP transport properties dramatically affect NP cell survival/function.
30721733	7	72	theme	viable	1357:1362	arg1	distance					1364:1371	a significantly shorter viable distance	1333:1371	a significantly shorter viable distance from the CEP/nutrient interface	1333:1403	RESULTS Solute diffusivity varied nearly 4-fold amongst the CEPs studied, and chambers with the least permeable CEPs appeared to have lower aggrecan, collagen-2, and matrix metalloproteinase-2 gene expression, as well as a significantly shorter viable distance from the CEP/nutrient interface.
30721733	4	73	theme	open	667:670	arg1	sides					672:676	the open sides	663:676	the open sides of diffusion chambers	663:698	DESIGN Human CEP tissues harvested from six fresh cadaveric lumbar spines (38-66 years old) were placed at the open sides of diffusion chambers.
30721733	7	74	theme	permeable	1214:1222	arg1	CEPs					1224:1227	the least permeable CEPs	1204:1227	the least permeable CEPs	1204:1227	RESULTS Solute diffusivity varied nearly 4-fold amongst the CEPs studied, and chambers with the least permeable CEPs appeared to have lower aggrecan, collagen-2, and matrix metalloproteinase-2 gene expression, as well as a significantly shorter viable distance from the CEP/nutrient interface.
30721733	0	75	theme	transport	67:75	arg1	properties					77:86	the transport properties	63:86	the transport properties of the cartilage endplate	63:112	Nutrient supply and nucleus pulposus cell function: effects of the transport properties of the cartilage endplate and potential implications for intradiscal biologic therapy.
30721733	2	76	theme	transport	370:378	arg1	properties					380:389	the transport properties	366:389	the transport properties of the cartilage endplate (CEP)	366:421	However, these therapies require a rich nutrient supply, which may be limited by the transport properties of the cartilage endplate (CEP).
30721733	5	77	theme	pulposus	716:723	arg1	cells					730:734	Bovine nucleus pulposus (NP) cells	701:734	Bovine nucleus pulposus (NP) cells cultured inside the chambers	701:763	Bovine nucleus pulposus (NP) cells cultured inside the chambers were nourished exclusively by nutrients diffusing through the CEP tissues.
30721733	9	78	theme	cross-link	1789:1798	arg1	maturity					1800:1807	lower cross-link maturity	1783:1807	lower cross-link maturity	1783:1807	Solute diffusivity in the CEP was associated with biochemical composition: low-diffusivity CEPs had greater amounts of collagen and aggrecan, more mineral, and lower cross-link maturity.
30721733	0	79	theme	cartilage	95:103	arg1	endplate					105:112	the cartilage endplate	91:112	the cartilage endplate	91:112	Nutrient supply and nucleus pulposus cell function: effects of the transport properties of the cartilage endplate and potential implications for intradiscal biologic therapy.
30721733	4	80	theme	cadaveric	606:614	arg1	spines					623:628	six fresh cadaveric lumbar spines	596:628	six fresh cadaveric lumbar spines (38-66 years old)	596:646	DESIGN Human CEP tissues harvested from six fresh cadaveric lumbar spines (38-66 years old) were placed at the open sides of diffusion chambers.
30721733	11	81	theme	biologic	1962:1969	arg1	therapies					1971:1979	biologic therapies	1962:1979	biologic therapies that require increased nutrient supply	1962:2018	Degeneration-related CEP matrix changes could hinder the success of biologic therapies that require increased nutrient supply.
30721733	6	82	theme	infrared	1082:1089	arg1	spectroscopy					1098:1109	infrared (FTIR) spectroscopy	1082:1109	infrared (FTIR) spectroscopy	1082:1109	After 72 h in culture, depth-dependent NP cell viability and gene expression were measured, and related to CEP transport properties and biochemical composition determined using fluorescence recovery after photobleaching and Fourier transform infrared (FTIR) spectroscopy.
30721733	4	83	theme	Human	563:567	arg1	tissues					573:579	DESIGN Human CEP tissues	556:579	DESIGN Human CEP tissues harvested from six fresh cadaveric lumbar spines (38-66 years old)	556:646	DESIGN Human CEP tissues harvested from six fresh cadaveric lumbar spines (38-66 years old) were placed at the open sides of diffusion chambers.
30721733	0	84	theme	nucleus	20:26	arg1	function					42:49	nucleus pulposus cell function	20:49	nucleus pulposus cell function	20:49	Nutrient supply and nucleus pulposus cell function: effects of the transport properties of the cartilage endplate and potential implications for intradiscal biologic therapy.
30721733	3	85	theme	CEP	468:470	arg1	properties					482:491	CEP transport properties	468:491	CEP transport properties	468:491	This study investigated how fluctuations in CEP transport properties impact nutrient diffusion and disc cell survival and function.
30721733	0	86	theme	cell	37:40	arg1	function					42:49	nucleus pulposus cell function	20:49	nucleus pulposus cell function	20:49	Nutrient supply and nucleus pulposus cell function: effects of the transport properties of the cartilage endplate and potential implications for intradiscal biologic therapy.
30721733	7	87	theme	gene	1305:1308	arg1	expression					1310:1319	lower aggrecan, collagen-2, and matrix metalloproteinase-2 gene expression	1246:1319	lower aggrecan, collagen-2, and matrix metalloproteinase-2 gene expression	1246:1319	RESULTS Solute diffusivity varied nearly 4-fold amongst the CEPs studied, and chambers with the least permeable CEPs appeared to have lower aggrecan, collagen-2, and matrix metalloproteinase-2 gene expression, as well as a significantly shorter viable distance from the CEP/nutrient interface.
30721733	8	88	theme	Increasing	1406:1415	arg1	density					1430:1436	Increasing chamber cell density	1406:1436	Increasing chamber cell density	1406:1436	Increasing chamber cell density shortened the viable distance; however, this effect was lost for low-diffusivity CEPs, which suggests that these CEPs may not provide enough nutrient diffusion to satisfy cell demands.
30721733	2	89	theme	endplate	408:415	arg1	properties					380:389	the transport properties	366:389	the transport properties of the cartilage endplate (CEP)	366:421	However, these therapies require a rich nutrient supply, which may be limited by the transport properties of the cartilage endplate (CEP).
30721733	7	90	theme	matrix	1278:1283	arg1	metalloproteinase-2					1285:1303	matrix metalloproteinase-2	1278:1303	matrix metalloproteinase-2	1278:1303	RESULTS Solute diffusivity varied nearly 4-fold amongst the CEPs studied, and chambers with the least permeable CEPs appeared to have lower aggrecan, collagen-2, and matrix metalloproteinase-2 gene expression, as well as a significantly shorter viable distance from the CEP/nutrient interface.
30721733	5	91	theme	nucleus	708:714	arg1	NP					726:727	NP	726:727	NP	726:727	Bovine nucleus pulposus (NP) cells cultured inside the chambers were nourished exclusively by nutrients diffusing through the CEP tissues.
30721733	5	91	theme	nucleus	708:714	arg1	pulposus					716:723	Bovine nucleus pulposus	701:723	Bovine nucleus pulposus (NP) cells cultured inside the chambers	701:763	Bovine nucleus pulposus (NP) cells cultured inside the chambers were nourished exclusively by nutrients diffusing through the CEP tissues.
30721733	8	92	theme	cell	1425:1428	arg1	density					1430:1436	Increasing chamber cell density	1406:1436	Increasing chamber cell density	1406:1436	Increasing chamber cell density shortened the viable distance; however, this effect was lost for low-diffusivity CEPs, which suggests that these CEPs may not provide enough nutrient diffusion to satisfy cell demands.
30721733	3	93	theme	nutrient	500:507	arg1	diffusion					509:517	nutrient diffusion	500:517	nutrient diffusion	500:517	This study investigated how fluctuations in CEP transport properties impact nutrient diffusion and disc cell survival and function.
30721733	4	94	theme	lumbar	616:621	arg1	spines					623:628	six fresh cadaveric lumbar spines	596:628	six fresh cadaveric lumbar spines (38-66 years old)	596:646	DESIGN Human CEP tissues harvested from six fresh cadaveric lumbar spines (38-66 years old) were placed at the open sides of diffusion chambers.
30721733	9	95	contain	had	1719:1721	arg2	mineral					1770:1776	mineral	1770:1776	mineral	1770:1776	Solute diffusivity in the CEP was associated with biochemical composition: low-diffusivity CEPs had greater amounts of collagen and aggrecan, more mineral, and lower cross-link maturity.
30721733	9	95	contain	had	1719:1721	arg2	amounts					1731:1737	greater amounts	1723:1737	greater amounts of collagen and aggrecan	1723:1762	Solute diffusivity in the CEP was associated with biochemical composition: low-diffusivity CEPs had greater amounts of collagen and aggrecan, more mineral, and lower cross-link maturity.
30721733	9	95	contain	had	1719:1721	arg2	collagen					1742:1749	collagen	1742:1749	collagen	1742:1749	Solute diffusivity in the CEP was associated with biochemical composition: low-diffusivity CEPs had greater amounts of collagen and aggrecan, more mineral, and lower cross-link maturity.
30721733	9	95	contain	had	1719:1721	arg2	aggrecan					1755:1762	aggrecan	1755:1762	aggrecan	1755:1762	Solute diffusivity in the CEP was associated with biochemical composition: low-diffusivity CEPs had greater amounts of collagen and aggrecan, more mineral, and lower cross-link maturity.
30721733	9	95	contain	had	1719:1721	arg2	maturity					1800:1807	lower cross-link maturity	1783:1807	lower cross-link maturity	1783:1807	Solute diffusivity in the CEP was associated with biochemical composition: low-diffusivity CEPs had greater amounts of collagen and aggrecan, more mineral, and lower cross-link maturity.
30721733	9	95	contain	had	1719:1721	arg1	CEPs					1714:1717	low-diffusivity CEPs	1698:1717	low-diffusivity CEPs	1698:1717	Solute diffusivity in the CEP was associated with biochemical composition: low-diffusivity CEPs had greater amounts of collagen and aggrecan, more mineral, and lower cross-link maturity.
30721733	0	96	dep	supply	9:14	arg1	effects					52:58	effects	52:58	effects of the transport properties of the cartilage endplate	52:112	Nutrient supply and nucleus pulposus cell function: effects of the transport properties of the cartilage endplate and potential implications for intradiscal biologic therapy.
30721733	0	96	dep	supply	9:14	arg1	implications					128:139	potential implications	118:139	potential implications for intradiscal biologic therapy	118:172	Nutrient supply and nucleus pulposus cell function: effects of the transport properties of the cartilage endplate and potential implications for intradiscal biologic therapy.
30721733	8	97	theme	viable	1452:1457	arg1	distance					1459:1466	the viable distance	1448:1466	the viable distance	1448:1466	Increasing chamber cell density shortened the viable distance; however, this effect was lost for low-diffusivity CEPs, which suggests that these CEPs may not provide enough nutrient diffusion to satisfy cell demands.
30721733	7	98	contain	have	1241:1244	arg2	distance					1364:1371	a significantly shorter viable distance	1333:1371	a significantly shorter viable distance from the CEP/nutrient interface	1333:1403	RESULTS Solute diffusivity varied nearly 4-fold amongst the CEPs studied, and chambers with the least permeable CEPs appeared to have lower aggrecan, collagen-2, and matrix metalloproteinase-2 gene expression, as well as a significantly shorter viable distance from the CEP/nutrient interface.
30721733	7	98	contain	have	1241:1244	arg2	expression					1310:1319	lower aggrecan, collagen-2, and matrix metalloproteinase-2 gene expression	1246:1319	lower aggrecan, collagen-2, and matrix metalloproteinase-2 gene expression	1246:1319	RESULTS Solute diffusivity varied nearly 4-fold amongst the CEPs studied, and chambers with the least permeable CEPs appeared to have lower aggrecan, collagen-2, and matrix metalloproteinase-2 gene expression, as well as a significantly shorter viable distance from the CEP/nutrient interface.
30721733	7	98	contain	have	1241:1244	arg1	chambers					1190:1197	chambers	1190:1197	chambers with the least permeable CEPs	1190:1227	RESULTS Solute diffusivity varied nearly 4-fold amongst the CEPs studied, and chambers with the least permeable CEPs appeared to have lower aggrecan, collagen-2, and matrix metalloproteinase-2 gene expression, as well as a significantly shorter viable distance from the CEP/nutrient interface.
30721733	1	99	theme	intervertebral	251:264	arg1	degeneration					271:282	intervertebral disc degeneration	251:282	intervertebral disc degeneration	251:282	OBJECTIVE Intradiscal biologic therapy is a promising strategy for managing intervertebral disc degeneration.
30978486	0	0	theme	agents	103:108	arg1	rate					33:36	enhanced dissolution rate	12:36	enhanced dissolution rate	12:36	Eutexia for enhanced dissolution rate and anti-inflammatory activity of nonsteroidal anti-inflammatory agents: Caffeine as a melting point modulator.
30978486	0	0	theme	agents	103:108	arg1	activity					60:67	anti-inflammatory activity	42:67	anti-inflammatory activity	42:67	Eutexia for enhanced dissolution rate and anti-inflammatory activity of nonsteroidal anti-inflammatory agents: Caffeine as a melting point modulator.
30978486	5	1	theme	optimum	681:687	arg1	composition					698:708	the optimum eutectic composition	677:708	the optimum eutectic composition from phase diagram and Tamman's triangle	677:749	Each drug was co-ground with caffeine in various ratios and the products were characterized by thermal analysis to determine the optimum eutectic composition from phase diagram and Tamman's triangle.
30978486	6	2	theme	optimum	756:762	arg1	systems					764:770	The optimum systems	752:770	The optimum systems	752:770	The optimum systems were subjected to X-ray powder diffraction (XRPD), Fourier-transform infrared (FTIR) and dissolution studies.
30978486	5	3	theme	phase	715:719	arg1	diagram					721:727	phase diagram	715:727	phase diagram	715:727	Each drug was co-ground with caffeine in various ratios and the products were characterized by thermal analysis to determine the optimum eutectic composition from phase diagram and Tamman's triangle.
30978486	0	4	theme	anti-inflammatory	85:101	arg1	agents					103:108	nonsteroidal anti-inflammatory agents	72:108	nonsteroidal anti-inflammatory agents	72:108	Eutexia for enhanced dissolution rate and anti-inflammatory activity of nonsteroidal anti-inflammatory agents: Caffeine as a melting point modulator.
30978486	5	5	theme	eutectic	689:696	arg1	composition					698:708	the optimum eutectic composition	677:708	the optimum eutectic composition from phase diagram and Tamman's triangle	677:749	Each drug was co-ground with caffeine in various ratios and the products were characterized by thermal analysis to determine the optimum eutectic composition from phase diagram and Tamman's triangle.
30978486	7	6	theme	rat	1009:1011	arg1	edema					1017:1021	carrageenan induced rat paw edema	989:1021	carrageenan induced rat paw edema method	989:1028	Co-ground systems at dose ratio were also assessed for drug dissolution and anti-inflammatory effect using carrageenan induced rat paw edema method.
30978486	1	7	theme	nonsteroidal	170:181	arg1	NSAIDs					208:213	NSAIDs	208:213	NSAIDs	208:213	Fast dissolution of nonsteroidal anti-inflammatory drugs (NSAIDs) is a prerequisite from patient perspective.
30978486	1	7	theme	nonsteroidal	170:181	arg1	drugs					201:205	nonsteroidal anti-inflammatory drugs	170:205	nonsteroidal anti-inflammatory drugs (NSAIDs)	170:214	Fast dissolution of nonsteroidal anti-inflammatory drugs (NSAIDs) is a prerequisite from patient perspective.
30978486	7	8	theme	dose	903:906	arg1	ratio					908:912	dose ratio	903:912	dose ratio	903:912	Co-ground systems at dose ratio were also assessed for drug dissolution and anti-inflammatory effect using carrageenan induced rat paw edema method.
30978486	9	9	theme	minor	1242:1246	arg1	changes					1248:1254	minor changes	1242:1254	minor changes	1242:1254	Eutexia did not alter FTIR spectra with minor changes being recorded in XRPD patterns.
30978486	9	10	theme	FTIR	1224:1227	arg1	spectra					1229:1235	FTIR spectra	1224:1235	FTIR spectra	1224:1235	Eutexia did not alter FTIR spectra with minor changes being recorded in XRPD patterns.
30978486	7	11	theme	paw	1013:1015	arg1	edema					1017:1021	carrageenan induced rat paw edema	989:1021	carrageenan induced rat paw edema method	989:1028	Co-ground systems at dose ratio were also assessed for drug dissolution and anti-inflammatory effect using carrageenan induced rat paw edema method.
30978486	7	12	theme	carrageenan	989:999	arg1	edema					1017:1021	carrageenan induced rat paw edema	989:1021	carrageenan induced rat paw edema method	989:1028	Co-ground systems at dose ratio were also assessed for drug dissolution and anti-inflammatory effect using carrageenan induced rat paw edema method.
30978486	2	13	theme	acidic	303:308	arg1	compounds					310:318	slowly dissolving acidic compounds	285:318	slowly dissolving acidic compounds	285:318	However, most NSAIDs are slowly dissolving acidic compounds.
30978486	2	13	theme	acidic	303:308	arg1	NSAIDs					274:279	most NSAIDs	269:279	most NSAIDs	269:279	However, most NSAIDs are slowly dissolving acidic compounds.
30978486	12	14	theme	drug	1594:1597	arg1	dissolution					1599:1609	drug dissolution	1594:1609	drug dissolution	1594:1609	The study introduced caffeine as eutectic forming analgesic for fixed dose combination with NSAIDs to enhance drug dissolution and anti-inflammatory effect.
30978486	11	15	theme	enhanced	1447:1454	arg1	response					1474:1481	enhanced anti-inflammatory response	1447:1481	enhanced anti-inflammatory response	1447:1481	Dissolution enhancement was associated with enhanced anti-inflammatory response.
30978486	7	16	from	ratio	908:912	arg1	systems					892:898	Co-ground systems	882:898	Co-ground systems at dose ratio	882:912	Co-ground systems at dose ratio were also assessed for drug dissolution and anti-inflammatory effect using carrageenan induced rat paw edema method.
30978486	6	17	theme	infrared	841:848	arg1	studies					873:879	Fourier-transform infrared (FTIR) and dissolution studies	823:879	Fourier-transform infrared (FTIR) and dissolution studies	823:879	The optimum systems were subjected to X-ray powder diffraction (XRPD), Fourier-transform infrared (FTIR) and dissolution studies.
30978486	6	17	theme	infrared	841:848	arg1	diffraction					803:813	X-ray powder diffraction	790:813	X-ray powder diffraction (XRPD)	790:820	The optimum systems were subjected to X-ray powder diffraction (XRPD), Fourier-transform infrared (FTIR) and dissolution studies.
30978486	2	18	theme	dissolving	292:301	arg1	compounds					310:318	slowly dissolving acidic compounds	285:318	slowly dissolving acidic compounds	285:318	However, most NSAIDs are slowly dissolving acidic compounds.
30978486	2	18	theme	dissolving	292:301	arg1	NSAIDs					274:279	most NSAIDs	269:279	most NSAIDs	269:279	However, most NSAIDs are slowly dissolving acidic compounds.
30978486	3	19	theme	adjuvant	357:364	arg1	analgesic					347:355	a commonly used analgesic	331:355	a commonly used analgesic adjuvant with NSAIDs	331:376	Caffeine, a commonly used analgesic adjuvant with NSAIDs showed high potential as eutectic co-former for acidic compounds.
30978486	3	19	theme	adjuvant	357:364	arg1	Caffeine					321:328	Caffeine	321:328	Caffeine	321:328	Caffeine, a commonly used analgesic adjuvant with NSAIDs showed high potential as eutectic co-former for acidic compounds.
30978486	4	20	theme	caffeine	497:504	arg1	potential					484:492	eutectic forming potential	467:492	eutectic forming potential of caffeine with meloxicam, aceclofenac and flurbiprofen	467:549	The study investigated eutectic forming potential of caffeine with meloxicam, aceclofenac and flurbiprofen.
30978486	5	21	theme	thermal	647:653	arg1	analysis					655:662	thermal analysis	647:662	thermal analysis to determine the optimum eutectic composition from phase diagram and Tamman's triangle	647:749	Each drug was co-ground with caffeine in various ratios and the products were characterized by thermal analysis to determine the optimum eutectic composition from phase diagram and Tamman's triangle.
30978486	12	22	theme	anti-inflammatory	1615:1631	arg1	effect					1633:1638	anti-inflammatory effect	1615:1638	anti-inflammatory effect	1615:1638	The study introduced caffeine as eutectic forming analgesic for fixed dose combination with NSAIDs to enhance drug dissolution and anti-inflammatory effect.
30978486	1	23	theme	anti-inflammatory	183:199	arg1	NSAIDs					208:213	NSAIDs	208:213	NSAIDs	208:213	Fast dissolution of nonsteroidal anti-inflammatory drugs (NSAIDs) is a prerequisite from patient perspective.
30978486	1	23	theme	anti-inflammatory	183:199	arg1	drugs					201:205	nonsteroidal anti-inflammatory drugs	170:205	nonsteroidal anti-inflammatory drugs (NSAIDs)	170:214	Fast dissolution of nonsteroidal anti-inflammatory drugs (NSAIDs) is a prerequisite from patient perspective.
30978486	10	24	theme	fast	1350:1353	arg1	dissolution					1355:1365	fast dissolution	1350:1365	fast dissolution	1350:1365	The eutectic systems underwent fast liberation of drugs with fast dissolution being retained even at dose ratios.
30978486	5	25	with	co-ground	566:574	arg1	caffeine					581:588	caffeine	581:588	caffeine	581:588	Each drug was co-ground with caffeine in various ratios and the products were characterized by thermal analysis to determine the optimum eutectic composition from phase diagram and Tamman's triangle.
30978486	10	26	theme	eutectic	1293:1300	arg1	systems					1302:1308	The eutectic systems	1289:1308	The eutectic systems	1289:1308	The eutectic systems underwent fast liberation of drugs with fast dissolution being retained even at dose ratios.
30978486	1	27	theme	drugs	201:205	arg1	dissolution					155:165	Fast dissolution	150:165	Fast dissolution of nonsteroidal anti-inflammatory drugs (NSAIDs)	150:214	Fast dissolution of nonsteroidal anti-inflammatory drugs (NSAIDs) is a prerequisite from patient perspective.
30978486	1	27	theme	drugs	201:205	arg1	prerequisite					221:232	a prerequisite	219:232	a prerequisite from patient perspective	219:257	Fast dissolution of nonsteroidal anti-inflammatory drugs (NSAIDs) is a prerequisite from patient perspective.
30978486	3	28	theme	used	342:345	arg1	analgesic					347:355	a commonly used analgesic	331:355	a commonly used analgesic adjuvant with NSAIDs	331:376	Caffeine, a commonly used analgesic adjuvant with NSAIDs showed high potential as eutectic co-former for acidic compounds.
30978486	3	28	theme	used	342:345	arg1	Caffeine					321:328	Caffeine	321:328	Caffeine	321:328	Caffeine, a commonly used analgesic adjuvant with NSAIDs showed high potential as eutectic co-former for acidic compounds.
30978486	7	29	theme	Co-ground	882:890	arg1	systems					892:898	Co-ground systems	882:898	Co-ground systems at dose ratio	882:912	Co-ground systems at dose ratio were also assessed for drug dissolution and anti-inflammatory effect using carrageenan induced rat paw edema method.
30978486	3	30	theme	eutectic	403:410	arg1	co-former					412:420	eutectic co-former	403:420	eutectic co-former	403:420	Caffeine, a commonly used analgesic adjuvant with NSAIDs showed high potential as eutectic co-former for acidic compounds.
30978486	8	31	theme	thermal	1056:1062	arg1	analysis					1064:1071	thermal analysis	1056:1071	thermal analysis	1056:1071	Eutexia was confirmed by thermal analysis with the optimum composition being 1:1, 1:1 and 1:2 (NSAID: caffeine) for aceclofenac, flurbiprofen and meloxicam, respectively.
30978486	6	32	dep	infrared	841:848	arg1	FTIR					851:854	FTIR	851:854	FTIR	851:854	The optimum systems were subjected to X-ray powder diffraction (XRPD), Fourier-transform infrared (FTIR) and dissolution studies.
30978486	12	33	theme	fixed	1548:1552	arg1	combination					1559:1569	fixed dose combination	1548:1569	fixed dose combination with NSAIDs	1548:1581	The study introduced caffeine as eutectic forming analgesic for fixed dose combination with NSAIDs to enhance drug dissolution and anti-inflammatory effect.
30978486	0	34	theme	dissolution	21:31	arg1	rate					33:36	enhanced dissolution rate	12:36	enhanced dissolution rate	12:36	Eutexia for enhanced dissolution rate and anti-inflammatory activity of nonsteroidal anti-inflammatory agents: Caffeine as a melting point modulator.
30978486	8	35	dep	NSAID	1126:1130	arg1	caffeine					1133:1140	caffeine	1133:1140	NSAID: caffeine	1126:1140	Eutexia was confirmed by thermal analysis with the optimum composition being 1:1, 1:1 and 1:2 (NSAID: caffeine) for aceclofenac, flurbiprofen and meloxicam, respectively.
30978486	0	36	theme	point	133:137	arg1	modulator					139:147	a melting point modulator	123:147	a melting point modulator	123:147	Eutexia for enhanced dissolution rate and anti-inflammatory activity of nonsteroidal anti-inflammatory agents: Caffeine as a melting point modulator.
30978486	6	37	theme	Fourier-transform	823:839	arg1	studies					873:879	Fourier-transform infrared (FTIR) and dissolution studies	823:879	Fourier-transform infrared (FTIR) and dissolution studies	823:879	The optimum systems were subjected to X-ray powder diffraction (XRPD), Fourier-transform infrared (FTIR) and dissolution studies.
30978486	6	37	theme	Fourier-transform	823:839	arg1	diffraction					803:813	X-ray powder diffraction	790:813	X-ray powder diffraction (XRPD)	790:820	The optimum systems were subjected to X-ray powder diffraction (XRPD), Fourier-transform infrared (FTIR) and dissolution studies.
30978486	0	38	theme	enhanced	12:19	arg1	rate					33:36	enhanced dissolution rate	12:36	enhanced dissolution rate	12:36	Eutexia for enhanced dissolution rate and anti-inflammatory activity of nonsteroidal anti-inflammatory agents: Caffeine as a melting point modulator.
30978486	11	39	theme	Dissolution	1403:1413	arg1	enhancement					1415:1425	Dissolution enhancement	1403:1425	Dissolution enhancement	1403:1425	Dissolution enhancement was associated with enhanced anti-inflammatory response.
30978486	0	40	theme	melting	125:131	arg1	modulator					139:147	a melting point modulator	123:147	a melting point modulator	123:147	Eutexia for enhanced dissolution rate and anti-inflammatory activity of nonsteroidal anti-inflammatory agents: Caffeine as a melting point modulator.
30978486	4	41	theme	forming	476:482	arg1	potential					484:492	eutectic forming potential	467:492	eutectic forming potential of caffeine with meloxicam, aceclofenac and flurbiprofen	467:549	The study investigated eutectic forming potential of caffeine with meloxicam, aceclofenac and flurbiprofen.
30978486	11	42	theme	anti-inflammatory	1456:1472	arg1	response					1474:1481	enhanced anti-inflammatory response	1447:1481	enhanced anti-inflammatory response	1447:1481	Dissolution enhancement was associated with enhanced anti-inflammatory response.
30978486	7	43	theme	induced	1001:1007	arg1	edema					1017:1021	carrageenan induced rat paw edema	989:1021	carrageenan induced rat paw edema method	989:1028	Co-ground systems at dose ratio were also assessed for drug dissolution and anti-inflammatory effect using carrageenan induced rat paw edema method.
30978486	10	44	theme	drugs	1339:1343	arg1	liberation					1325:1334	fast liberation	1320:1334	fast liberation of drugs	1320:1343	The eutectic systems underwent fast liberation of drugs with fast dissolution being retained even at dose ratios.
30978486	3	45	theme	acidic	426:431	arg1	compounds					433:441	acidic compounds	426:441	acidic compounds	426:441	Caffeine, a commonly used analgesic adjuvant with NSAIDs showed high potential as eutectic co-former for acidic compounds.
30978486	12	46	theme	dose	1554:1557	arg1	combination					1559:1569	fixed dose combination	1548:1569	fixed dose combination with NSAIDs	1548:1581	The study introduced caffeine as eutectic forming analgesic for fixed dose combination with NSAIDs to enhance drug dissolution and anti-inflammatory effect.
30978486	4	47	theme	eutectic	467:474	arg1	potential					484:492	eutectic forming potential	467:492	eutectic forming potential of caffeine with meloxicam, aceclofenac and flurbiprofen	467:549	The study investigated eutectic forming potential of caffeine with meloxicam, aceclofenac and flurbiprofen.
30978486	3	48	with	adjuvant	357:364	arg1	NSAIDs					371:376	NSAIDs	371:376	NSAIDs	371:376	Caffeine, a commonly used analgesic adjuvant with NSAIDs showed high potential as eutectic co-former for acidic compounds.
30978486	10	49	theme	fast	1320:1323	arg1	liberation					1325:1334	fast liberation	1320:1334	fast liberation of drugs	1320:1343	The eutectic systems underwent fast liberation of drugs with fast dissolution being retained even at dose ratios.
30978486	8	50	dep	1:2	1121:1123	arg1	NSAID					1126:1130	NSAID	1126:1130	NSAID: caffeine	1126:1140	Eutexia was confirmed by thermal analysis with the optimum composition being 1:1, 1:1 and 1:2 (NSAID: caffeine) for aceclofenac, flurbiprofen and meloxicam, respectively.
30978486	3	51	theme	high	385:388	arg1	potential					390:398	high potential	385:398	high potential as eutectic co-former for acidic compounds	385:441	Caffeine, a commonly used analgesic adjuvant with NSAIDs showed high potential as eutectic co-former for acidic compounds.
30978486	5	52	from	co-ground	566:574	arg1	ratios					601:606	various ratios	593:606	various ratios	593:606	Each drug was co-ground with caffeine in various ratios and the products were characterized by thermal analysis to determine the optimum eutectic composition from phase diagram and Tamman's triangle.
30978486	1	53	from	perspective	247:257	arg1	dissolution					155:165	Fast dissolution	150:165	Fast dissolution of nonsteroidal anti-inflammatory drugs (NSAIDs)	150:214	Fast dissolution of nonsteroidal anti-inflammatory drugs (NSAIDs) is a prerequisite from patient perspective.
30978486	1	53	from	perspective	247:257	arg1	prerequisite					221:232	a prerequisite	219:232	a prerequisite from patient perspective	219:257	Fast dissolution of nonsteroidal anti-inflammatory drugs (NSAIDs) is a prerequisite from patient perspective.
30978486	6	54	theme	powder	796:801	arg1	studies					873:879	Fourier-transform infrared (FTIR) and dissolution studies	823:879	Fourier-transform infrared (FTIR) and dissolution studies	823:879	The optimum systems were subjected to X-ray powder diffraction (XRPD), Fourier-transform infrared (FTIR) and dissolution studies.
30978486	6	54	theme	powder	796:801	arg1	XRPD					816:819	XRPD	816:819	XRPD	816:819	The optimum systems were subjected to X-ray powder diffraction (XRPD), Fourier-transform infrared (FTIR) and dissolution studies.
30978486	6	54	theme	powder	796:801	arg1	diffraction					803:813	X-ray powder diffraction	790:813	X-ray powder diffraction (XRPD)	790:820	The optimum systems were subjected to X-ray powder diffraction (XRPD), Fourier-transform infrared (FTIR) and dissolution studies.
30978486	0	55	theme	anti-inflammatory	42:58	arg1	activity					60:67	anti-inflammatory activity	42:67	anti-inflammatory activity	42:67	Eutexia for enhanced dissolution rate and anti-inflammatory activity of nonsteroidal anti-inflammatory agents: Caffeine as a melting point modulator.
30978486	12	56	theme	forming	1526:1532	arg1	analgesic					1534:1542	eutectic forming analgesic	1517:1542	eutectic forming analgesic	1517:1542	The study introduced caffeine as eutectic forming analgesic for fixed dose combination with NSAIDs to enhance drug dissolution and anti-inflammatory effect.
30978486	9	57	theme	XRPD	1274:1277	arg1	patterns					1279:1286	XRPD patterns	1274:1286	XRPD patterns	1274:1286	Eutexia did not alter FTIR spectra with minor changes being recorded in XRPD patterns.
30978486	6	58	theme	X-ray	790:794	arg1	studies					873:879	Fourier-transform infrared (FTIR) and dissolution studies	823:879	Fourier-transform infrared (FTIR) and dissolution studies	823:879	The optimum systems were subjected to X-ray powder diffraction (XRPD), Fourier-transform infrared (FTIR) and dissolution studies.
30978486	6	58	theme	X-ray	790:794	arg1	XRPD					816:819	XRPD	816:819	XRPD	816:819	The optimum systems were subjected to X-ray powder diffraction (XRPD), Fourier-transform infrared (FTIR) and dissolution studies.
30978486	6	58	theme	X-ray	790:794	arg1	diffraction					803:813	X-ray powder diffraction	790:813	X-ray powder diffraction (XRPD)	790:820	The optimum systems were subjected to X-ray powder diffraction (XRPD), Fourier-transform infrared (FTIR) and dissolution studies.
30978486	8	59	theme	optimum	1082:1088	arg1	1:1					1108:1110	1:1	1108:1110	1:1	1108:1110	Eutexia was confirmed by thermal analysis with the optimum composition being 1:1, 1:1 and 1:2 (NSAID: caffeine) for aceclofenac, flurbiprofen and meloxicam, respectively.
30978486	8	59	theme	optimum	1082:1088	arg1	composition					1090:1100	the optimum composition	1078:1100	the optimum composition	1078:1100	Eutexia was confirmed by thermal analysis with the optimum composition being 1:1, 1:1 and 1:2 (NSAID: caffeine) for aceclofenac, flurbiprofen and meloxicam, respectively.
30978486	7	60	theme	drug	937:940	arg1	dissolution					942:952	drug dissolution	937:952	drug dissolution	937:952	Co-ground systems at dose ratio were also assessed for drug dissolution and anti-inflammatory effect using carrageenan induced rat paw edema method.
30978486	7	61	theme	anti-inflammatory	958:974	arg1	effect					976:981	anti-inflammatory effect	958:981	anti-inflammatory effect	958:981	Co-ground systems at dose ratio were also assessed for drug dissolution and anti-inflammatory effect using carrageenan induced rat paw edema method.
30978486	6	62	theme	dissolution	861:871	arg1	studies					873:879	Fourier-transform infrared (FTIR) and dissolution studies	823:879	Fourier-transform infrared (FTIR) and dissolution studies	823:879	The optimum systems were subjected to X-ray powder diffraction (XRPD), Fourier-transform infrared (FTIR) and dissolution studies.
30978486	6	62	theme	dissolution	861:871	arg1	diffraction					803:813	X-ray powder diffraction	790:813	X-ray powder diffraction (XRPD)	790:820	The optimum systems were subjected to X-ray powder diffraction (XRPD), Fourier-transform infrared (FTIR) and dissolution studies.
30978486	5	63	theme	various	593:599	arg1	ratios					601:606	various ratios	593:606	various ratios	593:606	Each drug was co-ground with caffeine in various ratios and the products were characterized by thermal analysis to determine the optimum eutectic composition from phase diagram and Tamman's triangle.
30978486	5	64	from	ratios	601:606	arg1	co-ground					566:574	co-ground	566:574	co-ground	566:574	Each drug was co-ground with caffeine in various ratios and the products were characterized by thermal analysis to determine the optimum eutectic composition from phase diagram and Tamman's triangle.
30978486	2	65	theme	most	269:272	arg1	compounds					310:318	slowly dissolving acidic compounds	285:318	slowly dissolving acidic compounds	285:318	However, most NSAIDs are slowly dissolving acidic compounds.
30978486	2	65	theme	most	269:272	arg1	NSAIDs					274:279	most NSAIDs	269:279	most NSAIDs	269:279	However, most NSAIDs are slowly dissolving acidic compounds.
30978486	10	66	theme	dose	1390:1393	arg1	ratios					1395:1400	dose ratios	1390:1400	dose ratios	1390:1400	The eutectic systems underwent fast liberation of drugs with fast dissolution being retained even at dose ratios.
30978486	1	67	theme	patient	239:245	arg1	perspective					247:257	patient perspective	239:257	patient perspective	239:257	Fast dissolution of nonsteroidal anti-inflammatory drugs (NSAIDs) is a prerequisite from patient perspective.
30978486	0	68	theme	nonsteroidal	72:83	arg1	agents					103:108	nonsteroidal anti-inflammatory agents	72:108	nonsteroidal anti-inflammatory agents	72:108	Eutexia for enhanced dissolution rate and anti-inflammatory activity of nonsteroidal anti-inflammatory agents: Caffeine as a melting point modulator.
30978486	1	69	theme	Fast	150:153	arg1	dissolution					155:165	Fast dissolution	150:165	Fast dissolution of nonsteroidal anti-inflammatory drugs (NSAIDs)	150:214	Fast dissolution of nonsteroidal anti-inflammatory drugs (NSAIDs) is a prerequisite from patient perspective.
30978486	1	69	theme	Fast	150:153	arg1	prerequisite					221:232	a prerequisite	219:232	a prerequisite from patient perspective	219:257	Fast dissolution of nonsteroidal anti-inflammatory drugs (NSAIDs) is a prerequisite from patient perspective.
30978486	4	70	with	potential	484:492	arg1	meloxicam					511:519	meloxicam	511:519	meloxicam	511:519	The study investigated eutectic forming potential of caffeine with meloxicam, aceclofenac and flurbiprofen.
30978486	4	70	with	potential	484:492	arg1	flurbiprofen					538:549	flurbiprofen	538:549	flurbiprofen	538:549	The study investigated eutectic forming potential of caffeine with meloxicam, aceclofenac and flurbiprofen.
30978486	4	70	with	potential	484:492	arg1	aceclofenac					522:532	aceclofenac	522:532	aceclofenac	522:532	The study investigated eutectic forming potential of caffeine with meloxicam, aceclofenac and flurbiprofen.
30978486	12	71	with	combination	1559:1569	arg1	NSAIDs					1576:1581	NSAIDs	1576:1581	NSAIDs	1576:1581	The study introduced caffeine as eutectic forming analgesic for fixed dose combination with NSAIDs to enhance drug dissolution and anti-inflammatory effect.
30978486	0	72	dep	Eutexia	0:6	arg1	Caffeine					111:118	Caffeine	111:118	Eutexia for enhanced dissolution rate and anti-inflammatory activity of nonsteroidal anti-inflammatory agents: Caffeine as a melting point modulator.	0:148	Eutexia for enhanced dissolution rate and anti-inflammatory activity of nonsteroidal anti-inflammatory agents: Caffeine as a melting point modulator.
30978486	7	73	theme	edema	1017:1021	arg1	method					1023:1028	carrageenan induced rat paw edema method	989:1028	carrageenan induced rat paw edema method	989:1028	Co-ground systems at dose ratio were also assessed for drug dissolution and anti-inflammatory effect using carrageenan induced rat paw edema method.
30978486	12	74	theme	eutectic	1517:1524	arg1	analgesic					1534:1542	eutectic forming analgesic	1517:1542	eutectic forming analgesic	1517:1542	The study introduced caffeine as eutectic forming analgesic for fixed dose combination with NSAIDs to enhance drug dissolution and anti-inflammatory effect.
30978486	5	75	from	diagram	721:727	arg1	composition					698:708	the optimum eutectic composition	677:708	the optimum eutectic composition from phase diagram and Tamman's triangle	677:749	Each drug was co-ground with caffeine in various ratios and the products were characterized by thermal analysis to determine the optimum eutectic composition from phase diagram and Tamman's triangle.
30189258	8	0	theme	useful	1599:1604	arg1	information					1606:1616	some useful information	1594:1616	some useful information for the monitoring of EE2 activity in aquatic environment	1594:1674	The present results supplement the database for toxicity of EE2, especially for fish species endemic to China and provide some useful information for the monitoring of EE2 activity in aquatic environment.
30189258	8	1	theme	fish	1552:1555	arg1	species					1557:1563	fish species	1552:1563	fish species endemic to China	1552:1580	The present results supplement the database for toxicity of EE2, especially for fish species endemic to China and provide some useful information for the monitoring of EE2 activity in aquatic environment.
30189258	3	2	theme	Culter	511:516	arg1	alburnus					518:525	topmouth culter Culter alburnus	495:525	topmouth culter Culter alburnus	495:525	In the present study, one zona pellucida 3 (ZP3) homologue termed CaZP3 was firstly identified from topmouth culter Culter alburnus, following its structural characteristics, tissue distribution and transcriptional modulation to 17α-ethinylestradiol (EE2) exposure were investigated.
30189258	2	3	theme	risk	367:370	arg1	assessments					372:382	site-specific risk assessments	353:382	site-specific risk assessments	353:382	Information on the toxicity of estrogens to native species in that can be used in site-specific risk assessments is scarce.
30189258	3	4	theme	topmouth	495:502	arg1	alburnus					518:525	topmouth culter Culter alburnus	495:525	topmouth culter Culter alburnus	495:525	In the present study, one zona pellucida 3 (ZP3) homologue termed CaZP3 was firstly identified from topmouth culter Culter alburnus, following its structural characteristics, tissue distribution and transcriptional modulation to 17α-ethinylestradiol (EE2) exposure were investigated.
30189258	7	5	theme	female	1380:1385	arg1	culter					1387:1392	both male and female culter	1366:1392	both male and female culter	1366:1392	CaZP3 has the lower reactive ability to EE2 induction in comparison with VTG, however, CaZP3 transcripts were significantly induced in gonads of both male and female culter by EE2 and could be used as an alternative biomarker to monitor EE2 activity.
30189258	8	6	from	environment	1664:1674	arg1	monitoring					1626:1635	the monitoring	1622:1635	the monitoring of EE2 activity in aquatic environment	1622:1674	The present results supplement the database for toxicity of EE2, especially for fish species endemic to China and provide some useful information for the monitoring of EE2 activity in aquatic environment.
30189258	2	7	theme	estrogens	302:310	arg1	toxicity					290:297	the toxicity	286:297	the toxicity of estrogens to native species in that can be used in site-specific risk assessments	286:382	Information on the toxicity of estrogens to native species in that can be used in site-specific risk assessments is scarce.
30189258	8	8	theme	EE2	1640:1642	arg1	activity					1644:1651	EE2 activity	1640:1651	EE2 activity in aquatic environment	1640:1674	The present results supplement the database for toxicity of EE2, especially for fish species endemic to China and provide some useful information for the monitoring of EE2 activity in aquatic environment.
30189258	1	9	theme	development	184:194	arg1	disruption					161:170	disruption	161:170	disruption of physical development, reproduction and sexual behavior in aquatic wildlife, especially in fish	161:268	Estrogen could lead to abnormal modulation or disruption of physical development, reproduction and sexual behavior in aquatic wildlife, especially in fish.
30189258	1	9	theme	development	184:194	arg1	modulation					147:156	abnormal modulation	138:156	abnormal modulation	138:156	Estrogen could lead to abnormal modulation or disruption of physical development, reproduction and sexual behavior in aquatic wildlife, especially in fish.
30189258	1	10	theme	reproduction	197:208	arg1	disruption					161:170	disruption	161:170	disruption of physical development, reproduction and sexual behavior in aquatic wildlife, especially in fish	161:268	Estrogen could lead to abnormal modulation or disruption of physical development, reproduction and sexual behavior in aquatic wildlife, especially in fish.
30189258	1	10	theme	reproduction	197:208	arg1	modulation					147:156	abnormal modulation	138:156	abnormal modulation	138:156	Estrogen could lead to abnormal modulation or disruption of physical development, reproduction and sexual behavior in aquatic wildlife, especially in fish.
30189258	7	11	theme	CaZP3	1308:1312	arg1	biomarker					1437:1445	an alternative biomarker	1422:1445	an alternative biomarker to monitor EE2 activity	1422:1469	CaZP3 has the lower reactive ability to EE2 induction in comparison with VTG, however, CaZP3 transcripts were significantly induced in gonads of both male and female culter by EE2 and could be used as an alternative biomarker to monitor EE2 activity.
30189258	7	11	theme	CaZP3	1308:1312	arg1	transcripts					1314:1324	CaZP3 transcripts	1308:1324	CaZP3 transcripts	1308:1324	CaZP3 has the lower reactive ability to EE2 induction in comparison with VTG, however, CaZP3 transcripts were significantly induced in gonads of both male and female culter by EE2 and could be used as an alternative biomarker to monitor EE2 activity.
30189258	5	12	theme	introns	1028:1034	arg1	number					996:1001	equal number	990:1001	equal number of eight exons and seven introns with ZP3 counterparts of higher species	990:1074	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	12	theme	introns	1028:1034	arg1	EHP					880:882	EHP	880:882	EHP	880:882	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	12	theme	introns	1028:1034	arg1	domain					863:868	ZP domain	860:868	ZP domain	860:868	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	12	theme	introns	1028:1034	arg1	exons					1012:1016	eight exons	1006:1016	eight exons	1006:1016	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	12	theme	introns	1028:1034	arg1	site					934:937	one N-linked glycosylation site	907:937	one N-linked glycosylation site	907:937	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	12	theme	introns	1028:1034	arg1	SP					871:872	SP	871:872	SP	871:872	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	12	theme	introns	1028:1034	arg1	sites					980:984	two conserved O-linked glycosylation sites	943:984	two conserved O-linked glycosylation sites	943:984	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	12	theme	introns	1028:1034	arg1	residues					897:904	12 cysteine residues	885:904	12 cysteine residues	885:904	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	12	theme	introns	1028:1034	arg1	introns					1028:1034	seven introns	1022:1034	seven introns	1022:1034	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	12	theme	introns	1028:1034	arg1	IHP					875:877	IHP	875:877	IHP	875:877	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	3	13	theme	tissue	570:575	arg1	distribution					577:588	tissue distribution	570:588	tissue distribution	570:588	In the present study, one zona pellucida 3 (ZP3) homologue termed CaZP3 was firstly identified from topmouth culter Culter alburnus, following its structural characteristics, tissue distribution and transcriptional modulation to 17α-ethinylestradiol (EE2) exposure were investigated.
30189258	5	14	theme	functional	833:842	arg1	number					996:1001	equal number	990:1001	equal number of eight exons and seven introns with ZP3 counterparts of higher species	990:1074	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	14	theme	functional	833:842	arg1	EHP					880:882	EHP	880:882	EHP	880:882	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	14	theme	functional	833:842	arg1	domain					863:868	ZP domain	860:868	ZP domain	860:868	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	14	theme	functional	833:842	arg1	exons					1012:1016	eight exons	1006:1016	eight exons	1006:1016	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	14	theme	functional	833:842	arg1	site					934:937	one N-linked glycosylation site	907:937	one N-linked glycosylation site	907:937	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	14	theme	functional	833:842	arg1	SP					871:872	SP	871:872	SP	871:872	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	14	theme	functional	833:842	arg1	domains					844:850	analogical functional domains	822:850	analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species	822:1074	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	14	theme	functional	833:842	arg1	residues					897:904	12 cysteine residues	885:904	12 cysteine residues	885:904	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	14	theme	functional	833:842	arg1	introns					1028:1034	seven introns	1022:1034	seven introns	1022:1034	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	14	theme	functional	833:842	arg1	IHP					875:877	IHP	875:877	IHP	875:877	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	6	15	theme	male	1168:1171	arg1	muscle					1173:1178	male muscle	1168:1178	male muscle	1168:1178	CaZP3 mRNA predominantly expressed in ovary, besides, highly expressed in female heart and male muscle and relatively high expressed in testis.
30189258	4	16	theme	VTG	704:706	arg1	Meanwhile					679:687	Meanwhile	679:687	Meanwhile	679:687	Meanwhile, vitellogenin (VTG) gene was employed to provide a comparison of the reactive ability to EE2 induction.
30189258	4	16	theme	VTG	704:706	arg1	gene					709:712	vitellogenin (VTG) gene	690:712	vitellogenin (VTG) gene	690:712	Meanwhile, vitellogenin (VTG) gene was employed to provide a comparison of the reactive ability to EE2 induction.
30189258	1	17	from	wildlife	241:248	arg1	fish					265:268	fish	265:268	fish	265:268	Estrogen could lead to abnormal modulation or disruption of physical development, reproduction and sexual behavior in aquatic wildlife, especially in fish.
30189258	3	18	theme	characteristics	553:567	arg1	exposure					651:658	its structural characteristics, tissue distribution and transcriptional modulation to 17α-ethinylestradiol (EE2) exposure	538:658	its structural characteristics, tissue distribution and transcriptional modulation to 17α-ethinylestradiol (EE2) exposure were investigated	538:676	In the present study, one zona pellucida 3 (ZP3) homologue termed CaZP3 was firstly identified from topmouth culter Culter alburnus, following its structural characteristics, tissue distribution and transcriptional modulation to 17α-ethinylestradiol (EE2) exposure were investigated.
30189258	2	19	from	Information	271:281	arg1	toxicity					290:297	the toxicity	286:297	the toxicity of estrogens to native species in that can be used in site-specific risk assessments	286:382	Information on the toxicity of estrogens to native species in that can be used in site-specific risk assessments is scarce.
30189258	3	20	theme	modulation	610:619	arg1	exposure					651:658	its structural characteristics, tissue distribution and transcriptional modulation to 17α-ethinylestradiol (EE2) exposure	538:658	its structural characteristics, tissue distribution and transcriptional modulation to 17α-ethinylestradiol (EE2) exposure were investigated	538:676	In the present study, one zona pellucida 3 (ZP3) homologue termed CaZP3 was firstly identified from topmouth culter Culter alburnus, following its structural characteristics, tissue distribution and transcriptional modulation to 17α-ethinylestradiol (EE2) exposure were investigated.
30189258	5	21	theme	species	1068:1074	arg1	counterparts					1045:1056	ZP3 counterparts	1041:1056	ZP3 counterparts of higher species	1041:1074	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	1	22	from	disruption	161:170	arg1	wildlife					241:248	aquatic wildlife	233:248	aquatic wildlife	233:248	Estrogen could lead to abnormal modulation or disruption of physical development, reproduction and sexual behavior in aquatic wildlife, especially in fish.
30189258	1	23	theme	abnormal	138:145	arg1	modulation					147:156	abnormal modulation	138:156	abnormal modulation	138:156	Estrogen could lead to abnormal modulation or disruption of physical development, reproduction and sexual behavior in aquatic wildlife, especially in fish.
30189258	4	24	theme	vitellogenin	690:701	arg1	Meanwhile					679:687	Meanwhile	679:687	Meanwhile	679:687	Meanwhile, vitellogenin (VTG) gene was employed to provide a comparison of the reactive ability to EE2 induction.
30189258	4	24	theme	vitellogenin	690:701	arg1	gene					709:712	vitellogenin (VTG) gene	690:712	vitellogenin (VTG) gene	690:712	Meanwhile, vitellogenin (VTG) gene was employed to provide a comparison of the reactive ability to EE2 induction.
30189258	1	25	from	modulation	147:156	arg1	wildlife					241:248	aquatic wildlife	233:248	aquatic wildlife	233:248	Estrogen could lead to abnormal modulation or disruption of physical development, reproduction and sexual behavior in aquatic wildlife, especially in fish.
30189258	0	26	theme	topmouth	82:89	arg1	alburnus					105:112	adult topmouth culter Culter alburnus	76:112	adult topmouth culter Culter alburnus	76:112	Expression of zona pellucida 3 gene is regulated by 17α-ethinylestradiol in adult topmouth culter Culter alburnus.
30189258	5	27	theme	ZP3	1041:1043	arg1	counterparts					1045:1056	ZP3 counterparts	1041:1056	ZP3 counterparts of higher species	1041:1074	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	28	theme	N-linked	911:918	arg1	exons					1012:1016	eight exons	1006:1016	eight exons	1006:1016	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	28	theme	N-linked	911:918	arg1	introns					1028:1034	seven introns	1022:1034	seven introns	1022:1034	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	28	theme	N-linked	911:918	arg1	site					934:937	one N-linked glycosylation site	907:937	one N-linked glycosylation site	907:937	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	29	with	exons	1012:1016	arg1	counterparts					1045:1056	ZP3 counterparts	1041:1056	ZP3 counterparts of higher species	1041:1074	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	0	30	theme	Culter	98:103	arg1	alburnus					105:112	adult topmouth culter Culter alburnus	76:112	adult topmouth culter Culter alburnus	76:112	Expression of zona pellucida 3 gene is regulated by 17α-ethinylestradiol in adult topmouth culter Culter alburnus.
30189258	7	31	contain	has	1227:1229	arg2	ability					1250:1256	the lower reactive ability	1231:1256	the lower reactive ability to EE2 induction	1231:1273	CaZP3 has the lower reactive ability to EE2 induction in comparison with VTG, however, CaZP3 transcripts were significantly induced in gonads of both male and female culter by EE2 and could be used as an alternative biomarker to monitor EE2 activity.
30189258	7	31	contain	has	1227:1229	arg1	CaZP3					1221:1225	CaZP3	1221:1225	CaZP3	1221:1225	CaZP3 has the lower reactive ability to EE2 induction in comparison with VTG, however, CaZP3 transcripts were significantly induced in gonads of both male and female culter by EE2 and could be used as an alternative biomarker to monitor EE2 activity.
30189258	3	32	theme	present	402:408	arg1	study					410:414	the present study	398:414	the present study	398:414	In the present study, one zona pellucida 3 (ZP3) homologue termed CaZP3 was firstly identified from topmouth culter Culter alburnus, following its structural characteristics, tissue distribution and transcriptional modulation to 17α-ethinylestradiol (EE2) exposure were investigated.
30189258	5	33	theme	cysteine	888:895	arg1	residues					897:904	12 cysteine residues	885:904	12 cysteine residues	885:904	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	33	theme	cysteine	888:895	arg1	exons					1012:1016	eight exons	1006:1016	eight exons	1006:1016	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	33	theme	cysteine	888:895	arg1	introns					1028:1034	seven introns	1022:1034	seven introns	1022:1034	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	7	34	theme	EE2	1261:1263	arg1	induction					1265:1273	EE2 induction	1261:1273	EE2 induction	1261:1273	CaZP3 has the lower reactive ability to EE2 induction in comparison with VTG, however, CaZP3 transcripts were significantly induced in gonads of both male and female culter by EE2 and could be used as an alternative biomarker to monitor EE2 activity.
30189258	0	35	theme	zona	14:17	arg1	pellucida					19:27	zona pellucida 3	14:29	zona pellucida 3 gene	14:34	Expression of zona pellucida 3 gene is regulated by 17α-ethinylestradiol in adult topmouth culter Culter alburnus.
30189258	8	36	from	monitoring	1626:1635	arg1	environment					1664:1674	aquatic environment	1656:1674	aquatic environment	1656:1674	The present results supplement the database for toxicity of EE2, especially for fish species endemic to China and provide some useful information for the monitoring of EE2 activity in aquatic environment.
30189258	3	37	theme	zona	421:424	arg1	homologue					444:452	one zona pellucida 3 (ZP3) homologue	417:452	one zona pellucida 3 (ZP3) homologue termed CaZP3	417:465	In the present study, one zona pellucida 3 (ZP3) homologue termed CaZP3 was firstly identified from topmouth culter Culter alburnus, following its structural characteristics, tissue distribution and transcriptional modulation to 17α-ethinylestradiol (EE2) exposure were investigated.
30189258	7	38	used	used	1414:1417	arg2	transcripts					1314:1324	CaZP3 transcripts	1308:1324	CaZP3 transcripts	1308:1324	CaZP3 has the lower reactive ability to EE2 induction in comparison with VTG, however, CaZP3 transcripts were significantly induced in gonads of both male and female culter by EE2 and could be used as an alternative biomarker to monitor EE2 activity.
30189258	7	38	used	used	1414:1417	arg2	biomarker					1437:1445	an alternative biomarker	1422:1445	an alternative biomarker to monitor EE2 activity	1422:1469	CaZP3 has the lower reactive ability to EE2 induction in comparison with VTG, however, CaZP3 transcripts were significantly induced in gonads of both male and female culter by EE2 and could be used as an alternative biomarker to monitor EE2 activity.
30189258	5	39	theme	equal	990:994	arg1	number					996:1001	equal number	990:1001	equal number of eight exons and seven introns with ZP3 counterparts of higher species	990:1074	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	6	40	theme	CaZP3	1077:1081	arg1	mRNA					1083:1086	CaZP3 mRNA	1077:1086	CaZP3 mRNA predominantly expressed in ovary	1077:1119	CaZP3 mRNA predominantly expressed in ovary, besides, highly expressed in female heart and male muscle and relatively high expressed in testis.
30189258	4	41	theme	ability	767:773	arg1	comparison					740:749	a comparison	738:749	a comparison of the reactive ability to EE2 induction	738:790	Meanwhile, vitellogenin (VTG) gene was employed to provide a comparison of the reactive ability to EE2 induction.
30189258	3	42	theme	ZP3	439:441	arg1	homologue					444:452	one zona pellucida 3 (ZP3) homologue	417:452	one zona pellucida 3 (ZP3) homologue termed CaZP3	417:465	In the present study, one zona pellucida 3 (ZP3) homologue termed CaZP3 was firstly identified from topmouth culter Culter alburnus, following its structural characteristics, tissue distribution and transcriptional modulation to 17α-ethinylestradiol (EE2) exposure were investigated.
30189258	1	43	theme	behavior	221:228	arg1	disruption					161:170	disruption	161:170	disruption of physical development, reproduction and sexual behavior in aquatic wildlife, especially in fish	161:268	Estrogen could lead to abnormal modulation or disruption of physical development, reproduction and sexual behavior in aquatic wildlife, especially in fish.
30189258	1	43	theme	behavior	221:228	arg1	modulation					147:156	abnormal modulation	138:156	abnormal modulation	138:156	Estrogen could lead to abnormal modulation or disruption of physical development, reproduction and sexual behavior in aquatic wildlife, especially in fish.
30189258	4	44	theme	EE2	778:780	arg1	induction					782:790	EE2 induction	778:790	EE2 induction	778:790	Meanwhile, vitellogenin (VTG) gene was employed to provide a comparison of the reactive ability to EE2 induction.
30189258	1	45	theme	aquatic	233:239	arg1	wildlife					241:248	aquatic wildlife	233:248	aquatic wildlife	233:248	Estrogen could lead to abnormal modulation or disruption of physical development, reproduction and sexual behavior in aquatic wildlife, especially in fish.
30189258	5	46	theme	O-linked	957:964	arg1	sites					980:984	two conserved O-linked glycosylation sites	943:984	two conserved O-linked glycosylation sites	943:984	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	46	theme	O-linked	957:964	arg1	exons					1012:1016	eight exons	1006:1016	eight exons	1006:1016	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	46	theme	O-linked	957:964	arg1	introns					1028:1034	seven introns	1022:1034	seven introns	1022:1034	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	3	47	theme	culter	504:509	arg1	alburnus					518:525	topmouth culter Culter alburnus	495:525	topmouth culter Culter alburnus	495:525	In the present study, one zona pellucida 3 (ZP3) homologue termed CaZP3 was firstly identified from topmouth culter Culter alburnus, following its structural characteristics, tissue distribution and transcriptional modulation to 17α-ethinylestradiol (EE2) exposure were investigated.
30189258	7	48	theme	culter	1387:1392	arg1	gonads					1356:1361	gonads	1356:1361	gonads of both male and female culter	1356:1392	CaZP3 has the lower reactive ability to EE2 induction in comparison with VTG, however, CaZP3 transcripts were significantly induced in gonads of both male and female culter by EE2 and could be used as an alternative biomarker to monitor EE2 activity.
30189258	7	49	theme	male	1371:1374	arg1	culter					1387:1392	both male and female culter	1366:1392	both male and female culter	1366:1392	CaZP3 has the lower reactive ability to EE2 induction in comparison with VTG, however, CaZP3 transcripts were significantly induced in gonads of both male and female culter by EE2 and could be used as an alternative biomarker to monitor EE2 activity.
30189258	5	50	link	N-linked	911:918	arg1	exons					1012:1016	eight exons	1006:1016	eight exons	1006:1016	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	50	link	N-linked	911:918	arg1	introns					1028:1034	seven introns	1022:1034	seven introns	1022:1034	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	50	link	N-linked	911:918	arg1	site					934:937	one N-linked glycosylation site	907:937	one N-linked glycosylation site	907:937	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	1	51	theme	physical	175:182	arg1	development					184:194	physical development	175:194	physical development	175:194	Estrogen could lead to abnormal modulation or disruption of physical development, reproduction and sexual behavior in aquatic wildlife, especially in fish.
30189258	5	52	link	O-linked	957:964	arg1	sites					980:984	two conserved O-linked glycosylation sites	943:984	two conserved O-linked glycosylation sites	943:984	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	52	link	O-linked	957:964	arg1	exons					1012:1016	eight exons	1006:1016	eight exons	1006:1016	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	52	link	O-linked	957:964	arg1	introns					1028:1034	seven introns	1022:1034	seven introns	1022:1034	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	8	53	theme	activity	1644:1651	arg1	monitoring					1626:1635	the monitoring	1622:1635	the monitoring of EE2 activity in aquatic environment	1622:1674	The present results supplement the database for toxicity of EE2, especially for fish species endemic to China and provide some useful information for the monitoring of EE2 activity in aquatic environment.
30189258	5	54	theme	analogical	822:831	arg1	number					996:1001	equal number	990:1001	equal number of eight exons and seven introns with ZP3 counterparts of higher species	990:1074	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	54	theme	analogical	822:831	arg1	EHP					880:882	EHP	880:882	EHP	880:882	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	54	theme	analogical	822:831	arg1	domain					863:868	ZP domain	860:868	ZP domain	860:868	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	54	theme	analogical	822:831	arg1	exons					1012:1016	eight exons	1006:1016	eight exons	1006:1016	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	54	theme	analogical	822:831	arg1	site					934:937	one N-linked glycosylation site	907:937	one N-linked glycosylation site	907:937	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	54	theme	analogical	822:831	arg1	SP					871:872	SP	871:872	SP	871:872	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	54	theme	analogical	822:831	arg1	domains					844:850	analogical functional domains	822:850	analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species	822:1074	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	54	theme	analogical	822:831	arg1	residues					897:904	12 cysteine residues	885:904	12 cysteine residues	885:904	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	54	theme	analogical	822:831	arg1	introns					1028:1034	seven introns	1022:1034	seven introns	1022:1034	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	54	theme	analogical	822:831	arg1	IHP					875:877	IHP	875:877	IHP	875:877	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	0	55	from	17α-ethinylestradiol	52:71	arg1	alburnus					105:112	adult topmouth culter Culter alburnus	76:112	adult topmouth culter Culter alburnus	76:112	Expression of zona pellucida 3 gene is regulated by 17α-ethinylestradiol in adult topmouth culter Culter alburnus.
30189258	3	56	theme	structural	542:551	arg1	EE2					646:648	EE2	646:648	EE2	646:648	In the present study, one zona pellucida 3 (ZP3) homologue termed CaZP3 was firstly identified from topmouth culter Culter alburnus, following its structural characteristics, tissue distribution and transcriptional modulation to 17α-ethinylestradiol (EE2) exposure were investigated.
30189258	3	56	theme	structural	542:551	arg1	characteristics					553:567	its structural characteristics	538:567	its structural characteristics	538:567	In the present study, one zona pellucida 3 (ZP3) homologue termed CaZP3 was firstly identified from topmouth culter Culter alburnus, following its structural characteristics, tissue distribution and transcriptional modulation to 17α-ethinylestradiol (EE2) exposure were investigated.
30189258	8	57	theme	aquatic	1656:1662	arg1	environment					1664:1674	aquatic environment	1656:1674	aquatic environment	1656:1674	The present results supplement the database for toxicity of EE2, especially for fish species endemic to China and provide some useful information for the monitoring of EE2 activity in aquatic environment.
30189258	3	58	theme	transcriptional	594:608	arg1	modulation					610:619	transcriptional modulation	594:619	transcriptional modulation to 17α-ethinylestradiol	594:643	In the present study, one zona pellucida 3 (ZP3) homologue termed CaZP3 was firstly identified from topmouth culter Culter alburnus, following its structural characteristics, tissue distribution and transcriptional modulation to 17α-ethinylestradiol (EE2) exposure were investigated.
30189258	5	59	theme	ZP	860:861	arg1	domain					863:868	ZP domain	860:868	ZP domain	860:868	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	59	theme	ZP	860:861	arg1	exons					1012:1016	eight exons	1006:1016	eight exons	1006:1016	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	59	theme	ZP	860:861	arg1	introns					1028:1034	seven introns	1022:1034	seven introns	1022:1034	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	6	60	theme	female	1151:1156	arg1	heart					1158:1162	female heart	1151:1162	female heart	1151:1162	CaZP3 mRNA predominantly expressed in ovary, besides, highly expressed in female heart and male muscle and relatively high expressed in testis.
30189258	7	61	with	comparison	1278:1287	arg1	VTG					1294:1296	VTG	1294:1296	VTG	1294:1296	CaZP3 has the lower reactive ability to EE2 induction in comparison with VTG, however, CaZP3 transcripts were significantly induced in gonads of both male and female culter by EE2 and could be used as an alternative biomarker to monitor EE2 activity.
30189258	5	62	theme	exons	1012:1016	arg1	number					996:1001	equal number	990:1001	equal number of eight exons and seven introns with ZP3 counterparts of higher species	990:1074	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	62	theme	exons	1012:1016	arg1	EHP					880:882	EHP	880:882	EHP	880:882	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	62	theme	exons	1012:1016	arg1	domain					863:868	ZP domain	860:868	ZP domain	860:868	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	62	theme	exons	1012:1016	arg1	exons					1012:1016	eight exons	1006:1016	eight exons	1006:1016	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	62	theme	exons	1012:1016	arg1	site					934:937	one N-linked glycosylation site	907:937	one N-linked glycosylation site	907:937	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	62	theme	exons	1012:1016	arg1	SP					871:872	SP	871:872	SP	871:872	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	62	theme	exons	1012:1016	arg1	sites					980:984	two conserved O-linked glycosylation sites	943:984	two conserved O-linked glycosylation sites	943:984	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	62	theme	exons	1012:1016	arg1	residues					897:904	12 cysteine residues	885:904	12 cysteine residues	885:904	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	62	theme	exons	1012:1016	arg1	introns					1028:1034	seven introns	1022:1034	seven introns	1022:1034	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	62	theme	exons	1012:1016	arg1	IHP					875:877	IHP	875:877	IHP	875:877	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	3	63	theme	distribution	577:588	arg1	exposure					651:658	its structural characteristics, tissue distribution and transcriptional modulation to 17α-ethinylestradiol (EE2) exposure	538:658	its structural characteristics, tissue distribution and transcriptional modulation to 17α-ethinylestradiol (EE2) exposure were investigated	538:676	In the present study, one zona pellucida 3 (ZP3) homologue termed CaZP3 was firstly identified from topmouth culter Culter alburnus, following its structural characteristics, tissue distribution and transcriptional modulation to 17α-ethinylestradiol (EE2) exposure were investigated.
30189258	0	64	theme	adult	76:80	arg1	alburnus					105:112	adult topmouth culter Culter alburnus	76:112	adult topmouth culter Culter alburnus	76:112	Expression of zona pellucida 3 gene is regulated by 17α-ethinylestradiol in adult topmouth culter Culter alburnus.
30189258	5	65	gly	glycosylation	920:932	arg1	exons					1012:1016	eight exons	1006:1016	eight exons	1006:1016	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	65	gly	glycosylation	920:932	arg1	introns					1028:1034	seven introns	1022:1034	seven introns	1022:1034	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	65	gly	glycosylation	920:932	arg2	introns					1028:1034	seven introns	1022:1034	seven introns	1022:1034	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	65	gly	glycosylation	920:932	arg2	one					907:909	one	907:909	one	907:909	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	65	gly	glycosylation	920:932	arg2	site					934:937	one N-linked glycosylation site	907:937	one N-linked glycosylation site	907:937	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	65	gly	glycosylation	920:932	arg2	exons					1012:1016	eight exons	1006:1016	eight exons	1006:1016	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	0	66	theme	culter	91:96	arg1	alburnus					105:112	adult topmouth culter Culter alburnus	76:112	adult topmouth culter Culter alburnus	76:112	Expression of zona pellucida 3 gene is regulated by 17α-ethinylestradiol in adult topmouth culter Culter alburnus.
30189258	8	67	from	activity	1644:1651	arg1	environment					1664:1674	aquatic environment	1656:1674	aquatic environment	1656:1674	The present results supplement the database for toxicity of EE2, especially for fish species endemic to China and provide some useful information for the monitoring of EE2 activity in aquatic environment.
30189258	5	68	theme	glycosylation	920:932	arg1	exons					1012:1016	eight exons	1006:1016	eight exons	1006:1016	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	68	theme	glycosylation	920:932	arg1	introns					1028:1034	seven introns	1022:1034	seven introns	1022:1034	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	68	theme	glycosylation	920:932	arg1	site					934:937	one N-linked glycosylation site	907:937	one N-linked glycosylation site	907:937	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	69	theme	higher	1061:1066	arg1	species					1068:1074	higher species	1061:1074	higher species	1061:1074	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	70	gly	glycosylation	966:978	arg1	exons					1012:1016	eight exons	1006:1016	eight exons	1006:1016	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	70	gly	glycosylation	966:978	arg1	introns					1028:1034	seven introns	1022:1034	seven introns	1022:1034	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	70	gly	glycosylation	966:978	arg2	introns					1028:1034	seven introns	1022:1034	seven introns	1022:1034	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	70	gly	glycosylation	966:978	arg2	two					943:945	two	943:945	two	943:945	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	70	gly	glycosylation	966:978	arg2	sites					980:984	two conserved O-linked glycosylation sites	943:984	two conserved O-linked glycosylation sites	943:984	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	70	gly	glycosylation	966:978	arg2	exons					1012:1016	eight exons	1006:1016	eight exons	1006:1016	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	71	with	introns	1028:1034	arg1	counterparts					1045:1056	ZP3 counterparts	1041:1056	ZP3 counterparts of higher species	1041:1074	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	2	72	theme	site-specific	353:365	arg1	assessments					372:382	site-specific risk assessments	353:382	site-specific risk assessments	353:382	Information on the toxicity of estrogens to native species in that can be used in site-specific risk assessments is scarce.
30189258	0	73	theme	pellucida	19:27	arg1	gene					31:34	zona pellucida 3 gene	14:34	zona pellucida 3 gene	14:34	Expression of zona pellucida 3 gene is regulated by 17α-ethinylestradiol in adult topmouth culter Culter alburnus.
30189258	7	74	theme	reactive	1241:1248	arg1	ability					1250:1256	the lower reactive ability	1231:1256	the lower reactive ability to EE2 induction	1231:1273	CaZP3 has the lower reactive ability to EE2 induction in comparison with VTG, however, CaZP3 transcripts were significantly induced in gonads of both male and female culter by EE2 and could be used as an alternative biomarker to monitor EE2 activity.
30189258	8	75	theme	present	1476:1482	arg1	results					1484:1490	The present results	1472:1490	The present results	1472:1490	The present results supplement the database for toxicity of EE2, especially for fish species endemic to China and provide some useful information for the monitoring of EE2 activity in aquatic environment.
30189258	0	76	theme	gene	31:34	arg1	Expression					0:9	Expression	0:9	Expression of zona pellucida 3 gene	0:34	Expression of zona pellucida 3 gene is regulated by 17α-ethinylestradiol in adult topmouth culter Culter alburnus.
30189258	7	77	theme	EE2	1458:1460	arg1	activity					1462:1469	EE2 activity	1458:1469	EE2 activity	1458:1469	CaZP3 has the lower reactive ability to EE2 induction in comparison with VTG, however, CaZP3 transcripts were significantly induced in gonads of both male and female culter by EE2 and could be used as an alternative biomarker to monitor EE2 activity.
30189258	5	78	theme	glycosylation	966:978	arg1	sites					980:984	two conserved O-linked glycosylation sites	943:984	two conserved O-linked glycosylation sites	943:984	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	78	theme	glycosylation	966:978	arg1	exons					1012:1016	eight exons	1006:1016	eight exons	1006:1016	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	78	theme	glycosylation	966:978	arg1	introns					1028:1034	seven introns	1022:1034	seven introns	1022:1034	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	3	79	theme	pellucida	426:434	arg1	homologue					444:452	one zona pellucida 3 (ZP3) homologue	417:452	one zona pellucida 3 (ZP3) homologue termed CaZP3	417:465	In the present study, one zona pellucida 3 (ZP3) homologue termed CaZP3 was firstly identified from topmouth culter Culter alburnus, following its structural characteristics, tissue distribution and transcriptional modulation to 17α-ethinylestradiol (EE2) exposure were investigated.
30189258	8	80	theme	endemic	1565:1571	arg1	species					1557:1563	fish species	1552:1563	fish species endemic to China	1552:1580	The present results supplement the database for toxicity of EE2, especially for fish species endemic to China and provide some useful information for the monitoring of EE2 activity in aquatic environment.
30189258	7	81	theme	alternative	1425:1435	arg1	biomarker					1437:1445	an alternative biomarker	1422:1445	an alternative biomarker to monitor EE2 activity	1422:1469	CaZP3 has the lower reactive ability to EE2 induction in comparison with VTG, however, CaZP3 transcripts were significantly induced in gonads of both male and female culter by EE2 and could be used as an alternative biomarker to monitor EE2 activity.
30189258	7	81	theme	alternative	1425:1435	arg1	transcripts					1314:1324	CaZP3 transcripts	1308:1324	CaZP3 transcripts	1308:1324	CaZP3 has the lower reactive ability to EE2 induction in comparison with VTG, however, CaZP3 transcripts were significantly induced in gonads of both male and female culter by EE2 and could be used as an alternative biomarker to monitor EE2 activity.
30189258	4	82	theme	reactive	758:765	arg1	ability					767:773	the reactive ability	754:773	the reactive ability	754:773	Meanwhile, vitellogenin (VTG) gene was employed to provide a comparison of the reactive ability to EE2 induction.
30189258	2	83	theme	native	315:320	arg1	species					322:328	native species	315:328	native species in that can be used in site-specific risk assessments	315:382	Information on the toxicity of estrogens to native species in that can be used in site-specific risk assessments is scarce.
30189258	1	84	theme	sexual	214:219	arg1	behavior					221:228	sexual behavior	214:228	sexual behavior	214:228	Estrogen could lead to abnormal modulation or disruption of physical development, reproduction and sexual behavior in aquatic wildlife, especially in fish.
30189258	5	85	theme	conserved	947:955	arg1	sites					980:984	two conserved O-linked glycosylation sites	943:984	two conserved O-linked glycosylation sites	943:984	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	85	theme	conserved	947:955	arg1	exons					1012:1016	eight exons	1006:1016	eight exons	1006:1016	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	5	85	theme	conserved	947:955	arg1	introns					1028:1034	seven introns	1022:1034	seven introns	1022:1034	The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species.
30189258	8	86	theme	EE2	1532:1534	arg1	toxicity					1520:1527	toxicity	1520:1527	toxicity	1520:1527	The present results supplement the database for toxicity of EE2, especially for fish species endemic to China and provide some useful information for the monitoring of EE2 activity in aquatic environment.
33654956	4	0	theme	impaired	759:766	arg1	release					777:783	impaired mucilage release	759:783	impaired mucilage release	759:783	This protocol covers growth of plants to produce seeds suitable for analysis, extraction of extruded mucilage using water and sodium carbonate (used for mutants with impaired mucilage release), and extraction of alcohol insoluble residue (AIR) from whole seeds.
33654956	1	1	theme	mucilage	163:170	arg1	quantity					151:158	a significant quantity	137:158	a significant quantity	137:158	Arabidopsis seed coat epidermal cells deposit a significant quantity of mucilage, composed of the cell wall components pectin, hemicellulose, and cellulose, into the apoplast during development.
33654956	6	2	theme	exchange	1099:1106	arg1	chromatography					1108:1121	high-performance anion exchange chromatography	1076:1121	high-performance anion exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD)	1076:1176	Sensitive and reproducible quantification of the resulting monosaccharides is achieved using high-performance anion exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD).
33654956	3	3	theme	seed	512:515	arg1	processes					536:544	seed coat developmental processes	512:544	seed coat developmental processes	512:544	Determining the monosaccharide composition of both extruded mucilage and whole seeds is an essential technique for characterizing seed coat developmental processes and mutants with altered mucilage composition.
33654956	0	4	from	Analysis	0:7	arg1	Seeds					68:72	Arabidopsis Seed Mucilageand Whole Seeds	33:72	Arabidopsis Seed Mucilageand Whole Seeds	33:72	Analysis of Monosaccharides from Arabidopsis Seed Mucilageand Whole Seeds Using HPAEC-PAD.
33654956	1	5	theme	epidermal	113:121	arg1	cells					123:127	Arabidopsis seed coat epidermal cells	91:127	Arabidopsis seed coat epidermal cells	91:127	Arabidopsis seed coat epidermal cells deposit a significant quantity of mucilage, composed of the cell wall components pectin, hemicellulose, and cellulose, into the apoplast during development.
33654956	4	6	from	seeds	848:852	arg1	extraction					671:680	extraction	671:680	extraction of extruded mucilage using water and sodium carbonate (used for mutants with impaired mucilage release)	671:784	This protocol covers growth of plants to produce seeds suitable for analysis, extraction of extruded mucilage using water and sodium carbonate (used for mutants with impaired mucilage release), and extraction of alcohol insoluble residue (AIR) from whole seeds.
33654956	4	6	from	seeds	848:852	arg1	AIR					832:834	AIR	832:834	AIR	832:834	This protocol covers growth of plants to produce seeds suitable for analysis, extraction of extruded mucilage using water and sodium carbonate (used for mutants with impaired mucilage release), and extraction of alcohol insoluble residue (AIR) from whole seeds.
33654956	4	6	from	seeds	848:852	arg1	analysis					661:668	analysis	661:668	analysis	661:668	This protocol covers growth of plants to produce seeds suitable for analysis, extraction of extruded mucilage using water and sodium carbonate (used for mutants with impaired mucilage release), and extraction of alcohol insoluble residue (AIR) from whole seeds.
33654956	4	6	from	seeds	848:852	arg1	extraction					791:800	extraction	791:800	extraction of alcohol insoluble residue (AIR) from whole seeds	791:852	This protocol covers growth of plants to produce seeds suitable for analysis, extraction of extruded mucilage using water and sodium carbonate (used for mutants with impaired mucilage release), and extraction of alcohol insoluble residue (AIR) from whole seeds.
33654956	4	6	from	seeds	848:852	arg1	residue					823:829	alcohol insoluble residue	805:829	alcohol insoluble residue (AIR) from whole seeds	805:852	This protocol covers growth of plants to produce seeds suitable for analysis, extraction of extruded mucilage using water and sodium carbonate (used for mutants with impaired mucilage release), and extraction of alcohol insoluble residue (AIR) from whole seeds.
33654956	3	7	with	processes	536:544	arg1	composition					580:590	altered mucilage composition	563:590	altered mucilage composition	563:590	Determining the monosaccharide composition of both extruded mucilage and whole seeds is an essential technique for characterizing seed coat developmental processes and mutants with altered mucilage composition.
33654956	4	8	theme	mucilage	768:775	arg1	release					777:783	impaired mucilage release	759:783	impaired mucilage release	759:783	This protocol covers growth of plants to produce seeds suitable for analysis, extraction of extruded mucilage using water and sodium carbonate (used for mutants with impaired mucilage release), and extraction of alcohol insoluble residue (AIR) from whole seeds.
33654956	3	9	theme	essential	473:481	arg1	technique					483:491	an essential technique	470:491	an essential technique for characterizing seed coat developmental processes and mutants with altered mucilage composition	470:590	Determining the monosaccharide composition of both extruded mucilage and whole seeds is an essential technique for characterizing seed coat developmental processes and mutants with altered mucilage composition.
33654956	6	10	theme	pulsed	1136:1141	arg1	HPAEC-PAD					1167:1175	HPAEC-PAD	1167:1175	HPAEC-PAD	1167:1175	Sensitive and reproducible quantification of the resulting monosaccharides is achieved using high-performance anion exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD).
33654956	6	10	theme	pulsed	1136:1141	arg1	detection					1156:1164	pulsed amperometric detection	1136:1164	pulsed amperometric detection (HPAEC-PAD)	1136:1176	Sensitive and reproducible quantification of the resulting monosaccharides is achieved using high-performance anion exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD).
33654956	5	11	theme	prepared	859:866	arg1	polysaccharides					868:882	The prepared polysaccharides	855:882	The prepared polysaccharides	855:882	The prepared polysaccharides are then hydrolyzed using sulfuric acid, which hydrolyses all polysaccharides including cellulose.
33654956	3	12	theme	extruded	433:440	arg1	mucilage					442:449	extruded mucilage	433:449	extruded mucilage	433:449	Determining the monosaccharide composition of both extruded mucilage and whole seeds is an essential technique for characterizing seed coat developmental processes and mutants with altered mucilage composition.
33654956	6	13	theme	reproducible	997:1008	arg1	quantification					1010:1023	Sensitive and reproducible quantification	983:1023	Sensitive and reproducible quantification of the resulting monosaccharides	983:1056	Sensitive and reproducible quantification of the resulting monosaccharides is achieved using high-performance anion exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD).
33654956	0	14	theme	Seed	45:48	arg1	Seeds					68:72	Arabidopsis Seed Mucilageand Whole Seeds	33:72	Arabidopsis Seed Mucilageand Whole Seeds	33:72	Analysis of Monosaccharides from Arabidopsis Seed Mucilageand Whole Seeds Using HPAEC-PAD.
33654956	4	15	theme	suitable	648:655	arg1	seeds					642:646	seeds	642:646	seeds suitable for analysis, extraction of extruded mucilage using water and sodium carbonate (used for mutants with impaired mucilage release), and extraction of alcohol insoluble residue (AIR) from whole seeds	642:852	This protocol covers growth of plants to produce seeds suitable for analysis, extraction of extruded mucilage using water and sodium carbonate (used for mutants with impaired mucilage release), and extraction of alcohol insoluble residue (AIR) from whole seeds.
33654956	4	16	from	extraction	671:680	arg1	seeds					848:852	whole seeds	842:852	whole seeds	842:852	This protocol covers growth of plants to produce seeds suitable for analysis, extraction of extruded mucilage using water and sodium carbonate (used for mutants with impaired mucilage release), and extraction of alcohol insoluble residue (AIR) from whole seeds.
33654956	3	17	theme	mucilage	442:449	arg1	composition					413:423	the monosaccharide composition	394:423	the monosaccharide composition of both extruded mucilage and whole seeds	394:465	Determining the monosaccharide composition of both extruded mucilage and whole seeds is an essential technique for characterizing seed coat developmental processes and mutants with altered mucilage composition.
33654956	3	18	theme	developmental	522:534	arg1	processes					536:544	seed coat developmental processes	512:544	seed coat developmental processes	512:544	Determining the monosaccharide composition of both extruded mucilage and whole seeds is an essential technique for characterizing seed coat developmental processes and mutants with altered mucilage composition.
33654956	1	19	theme	Arabidopsis	91:101	arg1	cells					123:127	Arabidopsis seed coat epidermal cells	91:127	Arabidopsis seed coat epidermal cells	91:127	Arabidopsis seed coat epidermal cells deposit a significant quantity of mucilage, composed of the cell wall components pectin, hemicellulose, and cellulose, into the apoplast during development.
33654956	6	20	theme	amperometric	1143:1154	arg1	HPAEC-PAD					1167:1175	HPAEC-PAD	1167:1175	HPAEC-PAD	1167:1175	Sensitive and reproducible quantification of the resulting monosaccharides is achieved using high-performance anion exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD).
33654956	6	20	theme	amperometric	1143:1154	arg1	detection					1156:1164	pulsed amperometric detection	1136:1164	pulsed amperometric detection (HPAEC-PAD)	1136:1176	Sensitive and reproducible quantification of the resulting monosaccharides is achieved using high-performance anion exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD).
33654956	4	21	theme	extruded	685:692	arg1	mucilage					694:701	extruded mucilage	685:701	extruded mucilage using water and sodium carbonate (used for mutants with impaired mucilage release)	685:784	This protocol covers growth of plants to produce seeds suitable for analysis, extraction of extruded mucilage using water and sodium carbonate (used for mutants with impaired mucilage release), and extraction of alcohol insoluble residue (AIR) from whole seeds.
33654956	3	22	theme	mucilage	571:578	arg1	composition					580:590	altered mucilage composition	563:590	altered mucilage composition	563:590	Determining the monosaccharide composition of both extruded mucilage and whole seeds is an essential technique for characterizing seed coat developmental processes and mutants with altered mucilage composition.
33654956	4	23	theme	whole	842:846	arg1	seeds					848:852	whole seeds	842:852	whole seeds	842:852	This protocol covers growth of plants to produce seeds suitable for analysis, extraction of extruded mucilage using water and sodium carbonate (used for mutants with impaired mucilage release), and extraction of alcohol insoluble residue (AIR) from whole seeds.
33654956	1	24	theme	seed	103:106	arg1	cells					123:127	Arabidopsis seed coat epidermal cells	91:127	Arabidopsis seed coat epidermal cells	91:127	Arabidopsis seed coat epidermal cells deposit a significant quantity of mucilage, composed of the cell wall components pectin, hemicellulose, and cellulose, into the apoplast during development.
33654956	4	25	from	analysis	661:668	arg1	seeds					848:852	whole seeds	842:852	whole seeds	842:852	This protocol covers growth of plants to produce seeds suitable for analysis, extraction of extruded mucilage using water and sodium carbonate (used for mutants with impaired mucilage release), and extraction of alcohol insoluble residue (AIR) from whole seeds.
33654956	1	26	theme	cell	189:192	arg1	components					199:208	the cell wall components	185:208	the cell wall components pectin, hemicellulose, and cellulose	185:245	Arabidopsis seed coat epidermal cells deposit a significant quantity of mucilage, composed of the cell wall components pectin, hemicellulose, and cellulose, into the apoplast during development.
33654956	1	26	theme	cell	189:192	arg1	hemicellulose					218:230	hemicellulose	218:230	hemicellulose	218:230	Arabidopsis seed coat epidermal cells deposit a significant quantity of mucilage, composed of the cell wall components pectin, hemicellulose, and cellulose, into the apoplast during development.
33654956	1	26	theme	cell	189:192	arg1	cellulose					237:245	cellulose	237:245	cellulose	237:245	Arabidopsis seed coat epidermal cells deposit a significant quantity of mucilage, composed of the cell wall components pectin, hemicellulose, and cellulose, into the apoplast during development.
33654956	1	26	theme	cell	189:192	arg1	pectin					210:215	pectin	210:215	pectin	210:215	Arabidopsis seed coat epidermal cells deposit a significant quantity of mucilage, composed of the cell wall components pectin, hemicellulose, and cellulose, into the apoplast during development.
33654956	6	27	theme	Sensitive	983:991	arg1	quantification					1010:1023	Sensitive and reproducible quantification	983:1023	Sensitive and reproducible quantification of the resulting monosaccharides	983:1056	Sensitive and reproducible quantification of the resulting monosaccharides is achieved using high-performance anion exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD).
33654956	1	28	theme	coat	108:111	arg1	cells					123:127	Arabidopsis seed coat epidermal cells	91:127	Arabidopsis seed coat epidermal cells	91:127	Arabidopsis seed coat epidermal cells deposit a significant quantity of mucilage, composed of the cell wall components pectin, hemicellulose, and cellulose, into the apoplast during development.
33654956	1	29	theme	wall	194:197	arg1	components					199:208	the cell wall components	185:208	the cell wall components pectin, hemicellulose, and cellulose	185:245	Arabidopsis seed coat epidermal cells deposit a significant quantity of mucilage, composed of the cell wall components pectin, hemicellulose, and cellulose, into the apoplast during development.
33654956	1	29	theme	wall	194:197	arg1	hemicellulose					218:230	hemicellulose	218:230	hemicellulose	218:230	Arabidopsis seed coat epidermal cells deposit a significant quantity of mucilage, composed of the cell wall components pectin, hemicellulose, and cellulose, into the apoplast during development.
33654956	1	29	theme	wall	194:197	arg1	cellulose					237:245	cellulose	237:245	cellulose	237:245	Arabidopsis seed coat epidermal cells deposit a significant quantity of mucilage, composed of the cell wall components pectin, hemicellulose, and cellulose, into the apoplast during development.
33654956	1	29	theme	wall	194:197	arg1	pectin					210:215	pectin	210:215	pectin	210:215	Arabidopsis seed coat epidermal cells deposit a significant quantity of mucilage, composed of the cell wall components pectin, hemicellulose, and cellulose, into the apoplast during development.
33654956	0	30	theme	Monosaccharides	12:26	arg1	Analysis					0:7	Analysis	0:7	Analysis of Monosaccharides from Arabidopsis Seed Mucilageand Whole Seeds	0:72	Analysis of Monosaccharides from Arabidopsis Seed Mucilageand Whole Seeds Using HPAEC-PAD.
33654956	2	31	theme	mature	291:296	arg1	seeds					298:302	mature seeds	291:302	mature seeds	291:302	When mature seeds are hydrated, mucilage extrudes to form a gelatinous capsule around the seed.
33654956	6	32	theme	anion	1093:1097	arg1	chromatography					1108:1121	high-performance anion exchange chromatography	1076:1121	high-performance anion exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD)	1076:1176	Sensitive and reproducible quantification of the resulting monosaccharides is achieved using high-performance anion exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD).
33654956	3	33	theme	monosaccharide	398:411	arg1	composition					413:423	the monosaccharide composition	394:423	the monosaccharide composition of both extruded mucilage and whole seeds	394:465	Determining the monosaccharide composition of both extruded mucilage and whole seeds is an essential technique for characterizing seed coat developmental processes and mutants with altered mucilage composition.
33654956	4	34	theme	residue	823:829	arg1	extraction					671:680	extraction	671:680	extraction of extruded mucilage using water and sodium carbonate (used for mutants with impaired mucilage release)	671:784	This protocol covers growth of plants to produce seeds suitable for analysis, extraction of extruded mucilage using water and sodium carbonate (used for mutants with impaired mucilage release), and extraction of alcohol insoluble residue (AIR) from whole seeds.
33654956	4	34	theme	residue	823:829	arg1	analysis					661:668	analysis	661:668	analysis	661:668	This protocol covers growth of plants to produce seeds suitable for analysis, extraction of extruded mucilage using water and sodium carbonate (used for mutants with impaired mucilage release), and extraction of alcohol insoluble residue (AIR) from whole seeds.
33654956	4	34	theme	residue	823:829	arg1	extraction					791:800	extraction	791:800	extraction of alcohol insoluble residue (AIR) from whole seeds	791:852	This protocol covers growth of plants to produce seeds suitable for analysis, extraction of extruded mucilage using water and sodium carbonate (used for mutants with impaired mucilage release), and extraction of alcohol insoluble residue (AIR) from whole seeds.
33654956	6	35	theme	high-performance	1076:1091	arg1	chromatography					1108:1121	high-performance anion exchange chromatography	1076:1121	high-performance anion exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD)	1076:1176	Sensitive and reproducible quantification of the resulting monosaccharides is achieved using high-performance anion exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD).
33654956	0	36	theme	Arabidopsis	33:43	arg1	Seeds					68:72	Arabidopsis Seed Mucilageand Whole Seeds	33:72	Arabidopsis Seed Mucilageand Whole Seeds	33:72	Analysis of Monosaccharides from Arabidopsis Seed Mucilageand Whole Seeds Using HPAEC-PAD.
33654956	4	37	theme	insoluble	813:821	arg1	residue					823:829	alcohol insoluble residue	805:829	alcohol insoluble residue (AIR) from whole seeds	805:852	This protocol covers growth of plants to produce seeds suitable for analysis, extraction of extruded mucilage using water and sodium carbonate (used for mutants with impaired mucilage release), and extraction of alcohol insoluble residue (AIR) from whole seeds.
33654956	4	37	theme	insoluble	813:821	arg1	AIR					832:834	AIR	832:834	AIR	832:834	This protocol covers growth of plants to produce seeds suitable for analysis, extraction of extruded mucilage using water and sodium carbonate (used for mutants with impaired mucilage release), and extraction of alcohol insoluble residue (AIR) from whole seeds.
33654956	3	38	theme	whole	455:459	arg1	seeds					461:465	whole seeds	455:465	whole seeds	455:465	Determining the monosaccharide composition of both extruded mucilage and whole seeds is an essential technique for characterizing seed coat developmental processes and mutants with altered mucilage composition.
33654956	0	39	theme	Whole	62:66	arg1	Seeds					68:72	Arabidopsis Seed Mucilageand Whole Seeds	33:72	Arabidopsis Seed Mucilageand Whole Seeds	33:72	Analysis of Monosaccharides from Arabidopsis Seed Mucilageand Whole Seeds Using HPAEC-PAD.
33654956	0	40	from	Seeds	68:72	arg1	Monosaccharides					12:26	Monosaccharides	12:26	Monosaccharides from Arabidopsis Seed Mucilageand Whole Seeds	12:72	Analysis of Monosaccharides from Arabidopsis Seed Mucilageand Whole Seeds Using HPAEC-PAD.
33654956	0	40	from	Seeds	68:72	arg1	Analysis					0:7	Analysis	0:7	Analysis of Monosaccharides from Arabidopsis Seed Mucilageand Whole Seeds	0:72	Analysis of Monosaccharides from Arabidopsis Seed Mucilageand Whole Seeds Using HPAEC-PAD.
33654956	5	41	theme	sulfuric	910:917	arg1	acid					919:922	sulfuric acid	910:922	sulfuric acid	910:922	The prepared polysaccharides are then hydrolyzed using sulfuric acid, which hydrolyses all polysaccharides including cellulose.
33654956	0	42	theme	Mucilageand	50:60	arg1	Seeds					68:72	Arabidopsis Seed Mucilageand Whole Seeds	33:72	Arabidopsis Seed Mucilageand Whole Seeds	33:72	Analysis of Monosaccharides from Arabidopsis Seed Mucilageand Whole Seeds Using HPAEC-PAD.
33654956	4	43	with	mutants	746:752	arg1	release					777:783	impaired mucilage release	759:783	impaired mucilage release	759:783	This protocol covers growth of plants to produce seeds suitable for analysis, extraction of extruded mucilage using water and sodium carbonate (used for mutants with impaired mucilage release), and extraction of alcohol insoluble residue (AIR) from whole seeds.
33654956	4	44	theme	sodium	719:724	arg1	carbonate					726:734	sodium carbonate	719:734	sodium carbonate (used for mutants with impaired mucilage release)	719:784	This protocol covers growth of plants to produce seeds suitable for analysis, extraction of extruded mucilage using water and sodium carbonate (used for mutants with impaired mucilage release), and extraction of alcohol insoluble residue (AIR) from whole seeds.
33654956	4	45	theme	mucilage	694:701	arg1	extraction					671:680	extraction	671:680	extraction of extruded mucilage using water and sodium carbonate (used for mutants with impaired mucilage release)	671:784	This protocol covers growth of plants to produce seeds suitable for analysis, extraction of extruded mucilage using water and sodium carbonate (used for mutants with impaired mucilage release), and extraction of alcohol insoluble residue (AIR) from whole seeds.
33654956	4	45	theme	mucilage	694:701	arg1	analysis					661:668	analysis	661:668	analysis	661:668	This protocol covers growth of plants to produce seeds suitable for analysis, extraction of extruded mucilage using water and sodium carbonate (used for mutants with impaired mucilage release), and extraction of alcohol insoluble residue (AIR) from whole seeds.
33654956	4	45	theme	mucilage	694:701	arg1	extraction					791:800	extraction	791:800	extraction of alcohol insoluble residue (AIR) from whole seeds	791:852	This protocol covers growth of plants to produce seeds suitable for analysis, extraction of extruded mucilage using water and sodium carbonate (used for mutants with impaired mucilage release), and extraction of alcohol insoluble residue (AIR) from whole seeds.
33654956	3	46	theme	altered	563:569	arg1	composition					580:590	altered mucilage composition	563:590	altered mucilage composition	563:590	Determining the monosaccharide composition of both extruded mucilage and whole seeds is an essential technique for characterizing seed coat developmental processes and mutants with altered mucilage composition.
33654956	4	47	theme	plants	624:629	arg1	growth					614:619	growth	614:619	growth of plants	614:629	This protocol covers growth of plants to produce seeds suitable for analysis, extraction of extruded mucilage using water and sodium carbonate (used for mutants with impaired mucilage release), and extraction of alcohol insoluble residue (AIR) from whole seeds.
33654956	1	48	theme	significant	139:149	arg1	quantity					151:158	a significant quantity	137:158	a significant quantity	137:158	Arabidopsis seed coat epidermal cells deposit a significant quantity of mucilage, composed of the cell wall components pectin, hemicellulose, and cellulose, into the apoplast during development.
33654956	4	49	theme	alcohol	805:811	arg1	residue					823:829	alcohol insoluble residue	805:829	alcohol insoluble residue (AIR) from whole seeds	805:852	This protocol covers growth of plants to produce seeds suitable for analysis, extraction of extruded mucilage using water and sodium carbonate (used for mutants with impaired mucilage release), and extraction of alcohol insoluble residue (AIR) from whole seeds.
33654956	4	49	theme	alcohol	805:811	arg1	AIR					832:834	AIR	832:834	AIR	832:834	This protocol covers growth of plants to produce seeds suitable for analysis, extraction of extruded mucilage using water and sodium carbonate (used for mutants with impaired mucilage release), and extraction of alcohol insoluble residue (AIR) from whole seeds.
33654956	3	50	theme	seeds	461:465	arg1	composition					413:423	the monosaccharide composition	394:423	the monosaccharide composition of both extruded mucilage and whole seeds	394:465	Determining the monosaccharide composition of both extruded mucilage and whole seeds is an essential technique for characterizing seed coat developmental processes and mutants with altered mucilage composition.
33654956	3	51	theme	coat	517:520	arg1	processes					536:544	seed coat developmental processes	512:544	seed coat developmental processes	512:544	Determining the monosaccharide composition of both extruded mucilage and whole seeds is an essential technique for characterizing seed coat developmental processes and mutants with altered mucilage composition.
33654956	6	52	theme	monosaccharides	1042:1056	arg1	quantification					1010:1023	Sensitive and reproducible quantification	983:1023	Sensitive and reproducible quantification of the resulting monosaccharides	983:1056	Sensitive and reproducible quantification of the resulting monosaccharides is achieved using high-performance anion exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD).
33654956	3	53	with	mutants	550:556	arg1	composition					580:590	altered mucilage composition	563:590	altered mucilage composition	563:590	Determining the monosaccharide composition of both extruded mucilage and whole seeds is an essential technique for characterizing seed coat developmental processes and mutants with altered mucilage composition.
33654956	1	54	dep	components	199:208	arg1	components					199:208	the cell wall components	185:208	the cell wall components pectin, hemicellulose, and cellulose	185:245	Arabidopsis seed coat epidermal cells deposit a significant quantity of mucilage, composed of the cell wall components pectin, hemicellulose, and cellulose, into the apoplast during development.
33654956	1	54	dep	components	199:208	arg1	hemicellulose					218:230	hemicellulose	218:230	hemicellulose	218:230	Arabidopsis seed coat epidermal cells deposit a significant quantity of mucilage, composed of the cell wall components pectin, hemicellulose, and cellulose, into the apoplast during development.
33654956	1	54	dep	components	199:208	arg1	cellulose					237:245	cellulose	237:245	cellulose	237:245	Arabidopsis seed coat epidermal cells deposit a significant quantity of mucilage, composed of the cell wall components pectin, hemicellulose, and cellulose, into the apoplast during development.
33654956	1	54	dep	components	199:208	arg1	pectin					210:215	pectin	210:215	pectin	210:215	Arabidopsis seed coat epidermal cells deposit a significant quantity of mucilage, composed of the cell wall components pectin, hemicellulose, and cellulose, into the apoplast during development.
33654956	4	55	from	extraction	791:800	arg1	seeds					848:852	whole seeds	842:852	whole seeds	842:852	This protocol covers growth of plants to produce seeds suitable for analysis, extraction of extruded mucilage using water and sodium carbonate (used for mutants with impaired mucilage release), and extraction of alcohol insoluble residue (AIR) from whole seeds.
33654956	2	56	theme	gelatinous	346:355	arg1	capsule					357:363	a gelatinous capsule	344:363	a gelatinous capsule around the seed	344:379	When mature seeds are hydrated, mucilage extrudes to form a gelatinous capsule around the seed.
33654956	6	57	theme	resulting	1032:1040	arg1	monosaccharides					1042:1056	the resulting monosaccharides	1028:1056	the resulting monosaccharides	1028:1056	Sensitive and reproducible quantification of the resulting monosaccharides is achieved using high-performance anion exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD).
29876399	0	0	theme	extracts	83:90	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction, texture analysis and polysaccharide epitope mapping data of sequential extracts of strawberry, apple, tomato and aubergine fruit parenchyma.
29876399	0	0	theme	extracts	83:90	arg1	analysis					20:27	texture analysis	12:27	texture analysis	12:27	Extraction, texture analysis and polysaccharide epitope mapping data of sequential extracts of strawberry, apple, tomato and aubergine fruit parenchyma.
29876399	0	0	theme	extracts	83:90	arg1	mapping					56:62	polysaccharide epitope mapping	33:62	polysaccharide epitope mapping	33:62	Extraction, texture analysis and polysaccharide epitope mapping data of sequential extracts of strawberry, apple, tomato and aubergine fruit parenchyma.
29876399	1	1	theme	parenchyma	351:360	arg1	systems					362:368	fruit parenchyma systems	345:368	fruit parenchyma systems" (Cornuault et al., 2018) [1]	345:398	The data included in this article are related to the research article entitled "Disentangling pectic homogalacturonan and rhamnogalacturonan-I polysaccharides: evidence for sub-populations in fruit parenchyma systems" (Cornuault et al., 2018) [1].
29876399	1	1	theme	parenchyma	351:360	arg1	Cornuault					372:380	Cornuault	372:380	Cornuault	372:380	The data included in this article are related to the research article entitled "Disentangling pectic homogalacturonan and rhamnogalacturonan-I polysaccharides: evidence for sub-populations in fruit parenchyma systems" (Cornuault et al., 2018) [1].
29876399	1	2	from	sub-populations	326:340	arg1	systems					362:368	fruit parenchyma systems	345:368	fruit parenchyma systems" (Cornuault et al., 2018) [1]	345:398	The data included in this article are related to the research article entitled "Disentangling pectic homogalacturonan and rhamnogalacturonan-I polysaccharides: evidence for sub-populations in fruit parenchyma systems" (Cornuault et al., 2018) [1].
29876399	1	2	from	sub-populations	326:340	arg1	Cornuault					372:380	Cornuault	372:380	Cornuault	372:380	The data included in this article are related to the research article entitled "Disentangling pectic homogalacturonan and rhamnogalacturonan-I polysaccharides: evidence for sub-populations in fruit parenchyma systems" (Cornuault et al., 2018) [1].
29876399	3	3	theme	textural	492:499	arg1	analysis					520:527	textural and immunochemical analysis	492:527	analysis	520:527	These datasets compile textural and immunochemical analysis of polysaccharides of four economically important fruit crops: tomato, strawberry, aubergine and apple with contrasting textures and related taxonomical origins.
29876399	6	4	theme	parenchyma	1028:1037	arg1	tissues					1039:1045	the fruit parenchyma tissues	1018:1045	the fruit parenchyma tissues	1018:1045	The two datasets are a basis to relate cell wall composition and organization to the mechanical properties of the fruit parenchyma tissues.
29876399	0	5	theme	sequential	72:81	arg1	extracts					83:90	sequential extracts	72:90	sequential extracts of strawberry, apple, tomato and aubergine fruit parenchyma	72:150	Extraction, texture analysis and polysaccharide epitope mapping data of sequential extracts of strawberry, apple, tomato and aubergine fruit parenchyma.
29876399	3	6	theme	contrasting	637:647	arg1	textures					649:656	contrasting textures	637:656	contrasting textures	637:656	These datasets compile textural and immunochemical analysis of polysaccharides of four economically important fruit crops: tomato, strawberry, aubergine and apple with contrasting textures and related taxonomical origins.
29876399	1	7	theme	Disentangling	233:245	arg1	homogalacturonan					254:269	"Disentangling pectic homogalacturonan and rhamnogalacturonan-I polysaccharides	232:310	homogalacturonan	254:269	The data included in this article are related to the research article entitled "Disentangling pectic homogalacturonan and rhamnogalacturonan-I polysaccharides: evidence for sub-populations in fruit parenchyma systems" (Cornuault et al., 2018) [1].
29876399	0	8	theme	strawberry	95:104	arg1	extracts					83:90	sequential extracts	72:90	sequential extracts of strawberry, apple, tomato and aubergine fruit parenchyma	72:150	Extraction, texture analysis and polysaccharide epitope mapping data of sequential extracts of strawberry, apple, tomato and aubergine fruit parenchyma.
29876399	4	9	theme	monoclonal	771:780	arg1	antibodies					782:791	characterized monoclonal antibodies	757:791	characterized monoclonal antibodies	757:791	Cell wall components and their extractability were assessed using characterized monoclonal antibodies.
29876399	1	10	theme	pectic	247:252	arg1	homogalacturonan					254:269	"Disentangling pectic homogalacturonan and rhamnogalacturonan-I polysaccharides	232:310	homogalacturonan	254:269	The data included in this article are related to the research article entitled "Disentangling pectic homogalacturonan and rhamnogalacturonan-I polysaccharides: evidence for sub-populations in fruit parenchyma systems" (Cornuault et al., 2018) [1].
29876399	4	11	theme	characterized	757:769	arg1	antibodies					782:791	characterized monoclonal antibodies	757:791	characterized monoclonal antibodies	757:791	Cell wall components and their extractability were assessed using characterized monoclonal antibodies.
29876399	4	12	theme	wall	696:699	arg1	components					701:710	Cell wall components	691:710	Cell wall components	691:710	Cell wall components and their extractability were assessed using characterized monoclonal antibodies.
29876399	0	13	theme	apple	107:111	arg1	extracts					83:90	sequential extracts	72:90	sequential extracts of strawberry, apple, tomato and aubergine fruit parenchyma	72:150	Extraction, texture analysis and polysaccharide epitope mapping data of sequential extracts of strawberry, apple, tomato and aubergine fruit parenchyma.
29876399	0	14	dep	strawberry	95:104	arg1	parenchyma					141:150	fruit parenchyma	135:150	fruit parenchyma	135:150	Extraction, texture analysis and polysaccharide epitope mapping data of sequential extracts of strawberry, apple, tomato and aubergine fruit parenchyma.
29876399	5	15	theme	mechanical	885:894	arg1	properties					896:905	the mechanical properties	881:905	the mechanical properties	881:905	In addition, textural data obtained for the four parenchyma systems show variations in the mechanical properties.
29876399	5	16	theme	textural	807:814	arg1	data					816:819	textural data	807:819	textural data obtained for the four parenchyma systems	807:860	In addition, textural data obtained for the four parenchyma systems show variations in the mechanical properties.
29876399	3	17	theme	polysaccharides	532:546	arg1	analysis					520:527	textural and immunochemical analysis	492:527	analysis	520:527	These datasets compile textural and immunochemical analysis of polysaccharides of four economically important fruit crops: tomato, strawberry, aubergine and apple with contrasting textures and related taxonomical origins.
29876399	6	18	theme	cell	947:950	arg1	composition					957:967	cell wall composition	947:967	cell wall composition	947:967	The two datasets are a basis to relate cell wall composition and organization to the mechanical properties of the fruit parenchyma tissues.
29876399	2	19	theme	important	429:437	arg1	properties					411:420	Cell wall properties	401:420	Cell wall properties	401:420	Cell wall properties are an important contributor to fruit texture.
29876399	2	19	theme	important	429:437	arg1	contributor					439:449	an important contributor	426:449	an important contributor to fruit texture	426:466	Cell wall properties are an important contributor to fruit texture.
29876399	3	20	theme	fruit	579:583	arg1	aubergine					612:620	aubergine	612:620	aubergine	612:620	These datasets compile textural and immunochemical analysis of polysaccharides of four economically important fruit crops: tomato, strawberry, aubergine and apple with contrasting textures and related taxonomical origins.
29876399	3	20	theme	fruit	579:583	arg1	strawberry					600:609	strawberry	600:609	strawberry	600:609	These datasets compile textural and immunochemical analysis of polysaccharides of four economically important fruit crops: tomato, strawberry, aubergine and apple with contrasting textures and related taxonomical origins.
29876399	3	20	theme	fruit	579:583	arg1	apple					626:630	apple	626:630	apple	626:630	These datasets compile textural and immunochemical analysis of polysaccharides of four economically important fruit crops: tomato, strawberry, aubergine and apple with contrasting textures and related taxonomical origins.
29876399	3	20	theme	fruit	579:583	arg1	tomato					592:597	tomato	592:597	tomato	592:597	These datasets compile textural and immunochemical analysis of polysaccharides of four economically important fruit crops: tomato, strawberry, aubergine and apple with contrasting textures and related taxonomical origins.
29876399	3	20	theme	fruit	579:583	arg1	crops					585:589	four economically important fruit crops	551:589	four economically important fruit crops: tomato, strawberry, aubergine and apple with contrasting textures and related taxonomical origins	551:688	These datasets compile textural and immunochemical analysis of polysaccharides of four economically important fruit crops: tomato, strawberry, aubergine and apple with contrasting textures and related taxonomical origins.
29876399	3	21	theme	crops	585:589	arg1	polysaccharides					532:546	polysaccharides	532:546	polysaccharides of four economically important fruit crops: tomato, strawberry, aubergine and apple with contrasting textures and related taxonomical origins	532:688	These datasets compile textural and immunochemical analysis of polysaccharides of four economically important fruit crops: tomato, strawberry, aubergine and apple with contrasting textures and related taxonomical origins.
29876399	5	22	theme	parenchyma	843:852	arg1	systems					854:860	the four parenchyma systems	834:860	the four parenchyma systems	834:860	In addition, textural data obtained for the four parenchyma systems show variations in the mechanical properties.
29876399	1	23	dep	"	369:369	arg1	systems					362:368	fruit parenchyma systems	345:368	fruit parenchyma systems" (Cornuault et al., 2018) [1]	345:398	The data included in this article are related to the research article entitled "Disentangling pectic homogalacturonan and rhamnogalacturonan-I polysaccharides: evidence for sub-populations in fruit parenchyma systems" (Cornuault et al., 2018) [1].
29876399	1	23	dep	"	369:369	arg1	Cornuault					372:380	Cornuault	372:380	Cornuault	372:380	The data included in this article are related to the research article entitled "Disentangling pectic homogalacturonan and rhamnogalacturonan-I polysaccharides: evidence for sub-populations in fruit parenchyma systems" (Cornuault et al., 2018) [1].
29876399	0	24	theme	tomato	114:119	arg1	extracts					83:90	sequential extracts	72:90	sequential extracts of strawberry, apple, tomato and aubergine fruit parenchyma	72:150	Extraction, texture analysis and polysaccharide epitope mapping data of sequential extracts of strawberry, apple, tomato and aubergine fruit parenchyma.
29876399	2	25	theme	wall	406:409	arg1	properties					411:420	Cell wall properties	401:420	Cell wall properties	401:420	Cell wall properties are an important contributor to fruit texture.
29876399	2	25	theme	wall	406:409	arg1	contributor					439:449	an important contributor	426:449	an important contributor to fruit texture	426:466	Cell wall properties are an important contributor to fruit texture.
29876399	0	26	theme	texture	12:18	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction, texture analysis and polysaccharide epitope mapping data of sequential extracts of strawberry, apple, tomato and aubergine fruit parenchyma.
29876399	0	26	theme	texture	12:18	arg1	analysis					20:27	texture analysis	12:27	texture analysis	12:27	Extraction, texture analysis and polysaccharide epitope mapping data of sequential extracts of strawberry, apple, tomato and aubergine fruit parenchyma.
29876399	3	27	theme	important	569:577	arg1	aubergine					612:620	aubergine	612:620	aubergine	612:620	These datasets compile textural and immunochemical analysis of polysaccharides of four economically important fruit crops: tomato, strawberry, aubergine and apple with contrasting textures and related taxonomical origins.
29876399	3	27	theme	important	569:577	arg1	strawberry					600:609	strawberry	600:609	strawberry	600:609	These datasets compile textural and immunochemical analysis of polysaccharides of four economically important fruit crops: tomato, strawberry, aubergine and apple with contrasting textures and related taxonomical origins.
29876399	3	27	theme	important	569:577	arg1	apple					626:630	apple	626:630	apple	626:630	These datasets compile textural and immunochemical analysis of polysaccharides of four economically important fruit crops: tomato, strawberry, aubergine and apple with contrasting textures and related taxonomical origins.
29876399	3	27	theme	important	569:577	arg1	tomato					592:597	tomato	592:597	tomato	592:597	These datasets compile textural and immunochemical analysis of polysaccharides of four economically important fruit crops: tomato, strawberry, aubergine and apple with contrasting textures and related taxonomical origins.
29876399	3	27	theme	important	569:577	arg1	crops					585:589	four economically important fruit crops	551:589	four economically important fruit crops: tomato, strawberry, aubergine and apple with contrasting textures and related taxonomical origins	551:688	These datasets compile textural and immunochemical analysis of polysaccharides of four economically important fruit crops: tomato, strawberry, aubergine and apple with contrasting textures and related taxonomical origins.
29876399	3	28	dep	crops	585:589	arg1	aubergine					612:620	aubergine	612:620	aubergine	612:620	These datasets compile textural and immunochemical analysis of polysaccharides of four economically important fruit crops: tomato, strawberry, aubergine and apple with contrasting textures and related taxonomical origins.
29876399	3	28	dep	crops	585:589	arg1	strawberry					600:609	strawberry	600:609	strawberry	600:609	These datasets compile textural and immunochemical analysis of polysaccharides of four economically important fruit crops: tomato, strawberry, aubergine and apple with contrasting textures and related taxonomical origins.
29876399	3	28	dep	crops	585:589	arg1	tomato					592:597	tomato	592:597	tomato	592:597	These datasets compile textural and immunochemical analysis of polysaccharides of four economically important fruit crops: tomato, strawberry, aubergine and apple with contrasting textures and related taxonomical origins.
29876399	3	28	dep	crops	585:589	arg1	crops					585:589	four economically important fruit crops	551:589	four economically important fruit crops: tomato, strawberry, aubergine and apple with contrasting textures and related taxonomical origins	551:688	These datasets compile textural and immunochemical analysis of polysaccharides of four economically important fruit crops: tomato, strawberry, aubergine and apple with contrasting textures and related taxonomical origins.
29876399	3	28	dep	crops	585:589	arg1	apple					626:630	apple	626:630	apple	626:630	These datasets compile textural and immunochemical analysis of polysaccharides of four economically important fruit crops: tomato, strawberry, aubergine and apple with contrasting textures and related taxonomical origins.
29876399	2	29	theme	Cell	401:404	arg1	properties					411:420	Cell wall properties	401:420	Cell wall properties	401:420	Cell wall properties are an important contributor to fruit texture.
29876399	2	29	theme	Cell	401:404	arg1	contributor					439:449	an important contributor	426:449	an important contributor to fruit texture	426:466	Cell wall properties are an important contributor to fruit texture.
29876399	0	30	theme	polysaccharide	33:46	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction, texture analysis and polysaccharide epitope mapping data of sequential extracts of strawberry, apple, tomato and aubergine fruit parenchyma.
29876399	0	30	theme	polysaccharide	33:46	arg1	mapping					56:62	polysaccharide epitope mapping	33:62	polysaccharide epitope mapping	33:62	Extraction, texture analysis and polysaccharide epitope mapping data of sequential extracts of strawberry, apple, tomato and aubergine fruit parenchyma.
29876399	0	31	theme	aubergine	125:133	arg1	extracts					83:90	sequential extracts	72:90	sequential extracts of strawberry, apple, tomato and aubergine fruit parenchyma	72:150	Extraction, texture analysis and polysaccharide epitope mapping data of sequential extracts of strawberry, apple, tomato and aubergine fruit parenchyma.
29876399	2	32	theme	fruit	454:458	arg1	texture					460:466	fruit texture	454:466	fruit texture	454:466	Cell wall properties are an important contributor to fruit texture.
29876399	6	33	theme	fruit	1022:1026	arg1	tissues					1039:1045	the fruit parenchyma tissues	1018:1045	the fruit parenchyma tissues	1018:1045	The two datasets are a basis to relate cell wall composition and organization to the mechanical properties of the fruit parenchyma tissues.
29876399	6	34	theme	wall	952:955	arg1	composition					957:967	cell wall composition	947:967	cell wall composition	947:967	The two datasets are a basis to relate cell wall composition and organization to the mechanical properties of the fruit parenchyma tissues.
29876399	1	35	theme	rhamnogalacturonan-I	275:294	arg1	polysaccharides					296:310	"Disentangling pectic homogalacturonan and rhamnogalacturonan-I polysaccharides	232:310	polysaccharides	296:310	The data included in this article are related to the research article entitled "Disentangling pectic homogalacturonan and rhamnogalacturonan-I polysaccharides: evidence for sub-populations in fruit parenchyma systems" (Cornuault et al., 2018) [1].
29876399	0	36	theme	epitope	48:54	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction, texture analysis and polysaccharide epitope mapping data of sequential extracts of strawberry, apple, tomato and aubergine fruit parenchyma.
29876399	0	36	theme	epitope	48:54	arg1	mapping					56:62	polysaccharide epitope mapping	33:62	polysaccharide epitope mapping	33:62	Extraction, texture analysis and polysaccharide epitope mapping data of sequential extracts of strawberry, apple, tomato and aubergine fruit parenchyma.
29876399	0	37	theme	fruit	135:139	arg1	parenchyma					141:150	fruit parenchyma	135:150	fruit parenchyma	135:150	Extraction, texture analysis and polysaccharide epitope mapping data of sequential extracts of strawberry, apple, tomato and aubergine fruit parenchyma.
29876399	1	38	dep	Cornuault	372:380	arg1	[1					396:397	[1	396:397	Cornuault et al., 2018) [1	372:397	The data included in this article are related to the research article entitled "Disentangling pectic homogalacturonan and rhamnogalacturonan-I polysaccharides: evidence for sub-populations in fruit parenchyma systems" (Cornuault et al., 2018) [1].
29876399	1	39	dep	related	191:197	arg1	data					157:160	The data	153:160	The data included in this article	153:185	The data included in this article are related to the research article entitled "Disentangling pectic homogalacturonan and rhamnogalacturonan-I polysaccharides: evidence for sub-populations in fruit parenchyma systems" (Cornuault et al., 2018) [1].
29876399	1	39	dep	related	191:197	arg1	related					191:197	related	191:197	related	191:197	The data included in this article are related to the research article entitled "Disentangling pectic homogalacturonan and rhamnogalacturonan-I polysaccharides: evidence for sub-populations in fruit parenchyma systems" (Cornuault et al., 2018) [1].
29876399	1	39	dep	related	191:197	arg1	evidence					313:320	evidence	313:320	evidence for sub-populations in fruit parenchyma systems" (Cornuault et al., 2018) [1]	313:398	The data included in this article are related to the research article entitled "Disentangling pectic homogalacturonan and rhamnogalacturonan-I polysaccharides: evidence for sub-populations in fruit parenchyma systems" (Cornuault et al., 2018) [1].
29876399	0	40	dep	analysis	20:27	arg1	data					64:67	data	64:67	data	64:67	Extraction, texture analysis and polysaccharide epitope mapping data of sequential extracts of strawberry, apple, tomato and aubergine fruit parenchyma.
29876399	5	41	from	variations	867:876	arg1	properties					896:905	the mechanical properties	881:905	the mechanical properties	881:905	In addition, textural data obtained for the four parenchyma systems show variations in the mechanical properties.
29876399	3	42	theme	related	662:668	arg1	origins					682:688	related taxonomical origins	662:688	related taxonomical origins	662:688	These datasets compile textural and immunochemical analysis of polysaccharides of four economically important fruit crops: tomato, strawberry, aubergine and apple with contrasting textures and related taxonomical origins.
29876399	6	43	theme	mechanical	993:1002	arg1	properties					1004:1013	the mechanical properties	989:1013	the mechanical properties of the fruit parenchyma tissues	989:1045	The two datasets are a basis to relate cell wall composition and organization to the mechanical properties of the fruit parenchyma tissues.
29876399	1	44	theme	research	206:213	arg1	article					215:221	the research article	202:221	the research article entitled "Disentangling pectic homogalacturonan and rhamnogalacturonan-I polysaccharides	202:310	The data included in this article are related to the research article entitled "Disentangling pectic homogalacturonan and rhamnogalacturonan-I polysaccharides: evidence for sub-populations in fruit parenchyma systems" (Cornuault et al., 2018) [1].
29876399	3	45	theme	taxonomical	670:680	arg1	origins					682:688	related taxonomical origins	662:688	related taxonomical origins	662:688	These datasets compile textural and immunochemical analysis of polysaccharides of four economically important fruit crops: tomato, strawberry, aubergine and apple with contrasting textures and related taxonomical origins.
29876399	3	46	theme	immunochemical	505:518	arg1	analysis					520:527	textural and immunochemical analysis	492:527	analysis	520:527	These datasets compile textural and immunochemical analysis of polysaccharides of four economically important fruit crops: tomato, strawberry, aubergine and apple with contrasting textures and related taxonomical origins.
29876399	1	47	theme	fruit	345:349	arg1	systems					362:368	fruit parenchyma systems	345:368	fruit parenchyma systems" (Cornuault et al., 2018) [1]	345:398	The data included in this article are related to the research article entitled "Disentangling pectic homogalacturonan and rhamnogalacturonan-I polysaccharides: evidence for sub-populations in fruit parenchyma systems" (Cornuault et al., 2018) [1].
29876399	1	47	theme	fruit	345:349	arg1	Cornuault					372:380	Cornuault	372:380	Cornuault	372:380	The data included in this article are related to the research article entitled "Disentangling pectic homogalacturonan and rhamnogalacturonan-I polysaccharides: evidence for sub-populations in fruit parenchyma systems" (Cornuault et al., 2018) [1].
29876399	3	48	with	crops	585:589	arg1	textures					649:656	contrasting textures	637:656	contrasting textures	637:656	These datasets compile textural and immunochemical analysis of polysaccharides of four economically important fruit crops: tomato, strawberry, aubergine and apple with contrasting textures and related taxonomical origins.
29876399	3	48	with	crops	585:589	arg1	origins					682:688	related taxonomical origins	662:688	related taxonomical origins	662:688	These datasets compile textural and immunochemical analysis of polysaccharides of four economically important fruit crops: tomato, strawberry, aubergine and apple with contrasting textures and related taxonomical origins.
29876399	4	49	theme	Cell	691:694	arg1	components					701:710	Cell wall components	691:710	Cell wall components	691:710	Cell wall components and their extractability were assessed using characterized monoclonal antibodies.
29876399	6	50	theme	tissues	1039:1045	arg1	properties					1004:1013	the mechanical properties	989:1013	the mechanical properties of the fruit parenchyma tissues	989:1045	The two datasets are a basis to relate cell wall composition and organization to the mechanical properties of the fruit parenchyma tissues.
30129919	2	0	dep	Gram-stain-positive	150:168	arg1	endospore-producing					179:197	endospore-producing	179:197	endospore-producing	179:197	A Gram-stain-positive, motile, endospore-producing, facultative anaerobic bacterial strain, designated ATCC 27380T, was isolated from heat-stressed soil of Cape Canaveral, Florida, USA.
30129919	2	0	dep	Gram-stain-positive	150:168	arg1	motile					171:176	motile	171:176	motile	171:176	A Gram-stain-positive, motile, endospore-producing, facultative anaerobic bacterial strain, designated ATCC 27380T, was isolated from heat-stressed soil of Cape Canaveral, Florida, USA.
30129919	2	0	dep	Gram-stain-positive	150:168	arg1	facultative					200:210	facultative	200:210	facultative	200:210	A Gram-stain-positive, motile, endospore-producing, facultative anaerobic bacterial strain, designated ATCC 27380T, was isolated from heat-stressed soil of Cape Canaveral, Florida, USA.
30129919	11	1	theme	ATCC	1481:1484	arg1	27380T					1486:1491	strain ATCC 27380T	1474:1491	strain ATCC 27380T	1474:1491	The average amino acid identity between strain ATCC 27380T and P. rigui JCM 16352T was 68.72 %, which was above the suggested genus level demarcation of 65 %.
30129919	10	2	theme	JCM	1287:1289	arg1	16352T					1291:1296	P. rigui JCM 16352T	1278:1296	P. rigui JCM 16352T	1278:1296	The average nucleotide identity and in silico DNA-DNA hybridization values between strain ATCC 27380T and P. rigui JCM 16352T were 72.5% and 18.5 %, respectively, which were below the threshold suggested for species differentiation (96% and 70 %, respectively).
30129919	3	3	theme	0.5-3 	439:444	arg1	%					445:445	%	445:445	%	445:445	Growth was observed at 20-42 °C (optimum, 37 °C), at pH 6.0-10.0 (optimum pH 7.0) and in the presence of 0.5-3 % NaCl (optimum 0.5 %).
30129919	7	4	theme	16S	805:807	arg1	sequencing					819:828	16S rRNA gene sequencing	805:828	16S rRNA gene sequencing	805:828	Phylogenetic analysis based on 16S rRNA gene sequencing affiliated strain ATCC 27380T to the genus Paenibacillus, and showed the highest sequence similarity to Paenibacillus rigui JCM 16352T (97.0 %).
30129919	9	5	theme	ATCC	1099:1102	arg1	27380T					1104:1109	ATCC 27380T	1099:1109	ATCC 27380T	1099:1109	The draft genome of ATCC 27380T had a size of 4,361,187 bases, with a G+C content of 51.0 %.
30129919	9	6	contain	had	1111:1113	arg1	genome					1089:1094	The draft genome	1079:1094	The draft genome of ATCC 27380T	1079:1109	The draft genome of ATCC 27380T had a size of 4,361,187 bases, with a G+C content of 51.0 %.
30129919	9	6	contain	had	1111:1113	arg2	size					1117:1120	a size	1115:1120	a size of 4,361,187 bases	1115:1139	The draft genome of ATCC 27380T had a size of 4,361,187 bases, with a G+C content of 51.0 %.
30129919	4	7	theme	was	574:576	arg1	acid					513:516	meso-diaminopimelic acid	493:516	meso-diaminopimelic acid	493:516	The cell wall contained meso-diaminopimelic acid as the diagnostic amino acid and the isoprenoid quinone was MK-7.
30129919	4	7	theme	was	574:576	arg1	MK-7					578:581	the isoprenoid quinone was MK-7	551:581	the isoprenoid quinone was MK-7	551:581	The cell wall contained meso-diaminopimelic acid as the diagnostic amino acid and the isoprenoid quinone was MK-7.
30129919	11	8	theme	rigui	1500:1504	arg1	16352T					1510:1515	P. rigui JCM 16352T	1497:1515	P. rigui JCM 16352T	1497:1515	The average amino acid identity between strain ATCC 27380T and P. rigui JCM 16352T was 68.72 %, which was above the suggested genus level demarcation of 65 %.
30129919	3	9	theme	optimum	453:459	arg1	%					465:465	optimum 0.5 %	453:465	optimum 0.5 %	453:465	Growth was observed at 20-42 °C (optimum, 37 °C), at pH 6.0-10.0 (optimum pH 7.0) and in the presence of 0.5-3 % NaCl (optimum 0.5 %).
30129919	3	9	theme	optimum	453:459	arg1	 NaCl					446:450	0.5-3 % NaCl	439:450	0.5-3 % NaCl (optimum 0.5 %)	439:466	Growth was observed at 20-42 °C (optimum, 37 °C), at pH 6.0-10.0 (optimum pH 7.0) and in the presence of 0.5-3 % NaCl (optimum 0.5 %).
30129919	7	10	theme	Phylogenetic	774:785	arg1	analysis					787:794	Phylogenetic analysis	774:794	Phylogenetic analysis based on 16S rRNA gene sequencing	774:828	Phylogenetic analysis based on 16S rRNA gene sequencing affiliated strain ATCC 27380T to the genus Paenibacillus, and showed the highest sequence similarity to Paenibacillus rigui JCM 16352T (97.0 %).
30129919	6	11	theme	fatty	724:728	arg1	acids					730:734	The main fatty acids	715:734	The main fatty acids	715:734	The main fatty acids were iso-C15 : 0 and anteiso-C15 : 0.
30129919	6	11	theme	fatty	724:728	arg1	iso-C15 					741:748	iso-C15 	741:748	iso-C15 	741:748	The main fatty acids were iso-C15 : 0 and anteiso-C15 : 0.
30129919	12	12	from	species	1687:1693	arg1	Paenibacillus					1708:1720	Paenibacillus	1708:1720	Paenibacillus	1708:1720	Based on phenotypic, genotypic and chemotaxonomic data, strain ATCC 27380T represents a novel species in the genus Paenibacillus, for which the name Paenibacillusxerothermodurans sp.
30129919	2	13	theme	anaerobic	212:220	arg1	strain					232:237	A Gram-stain-positive, motile, endospore-producing, facultative anaerobic bacterial strain	148:237	A Gram-stain-positive, motile, endospore-producing, facultative anaerobic bacterial strain	148:237	A Gram-stain-positive, motile, endospore-producing, facultative anaerobic bacterial strain, designated ATCC 27380T, was isolated from heat-stressed soil of Cape Canaveral, Florida, USA.
30129919	6	14	dep	iso-C15 	741:748	arg1	 0					770:771	 0	770:771	iso-C15 : 0 and anteiso-C15 : 0	741:771	The main fatty acids were iso-C15 : 0 and anteiso-C15 : 0.
30129919	6	14	dep	iso-C15 	741:748	arg1	anteiso-C15 					757:768	anteiso-C15 	757:768	anteiso-C15 	757:768	The main fatty acids were iso-C15 : 0 and anteiso-C15 : 0.
30129919	6	14	dep	iso-C15 	741:748	arg1	 0					750:751	 0	750:751	 0	750:751	The main fatty acids were iso-C15 : 0 and anteiso-C15 : 0.
30129919	7	15	theme	Paenibacillus	934:946	arg1	%					971:971	97.0 %	966:971	97.0 %	966:971	Phylogenetic analysis based on 16S rRNA gene sequencing affiliated strain ATCC 27380T to the genus Paenibacillus, and showed the highest sequence similarity to Paenibacillus rigui JCM 16352T (97.0 %).
30129919	7	15	theme	Paenibacillus	934:946	arg1	16352T					958:963	Paenibacillus rigui JCM 16352T	934:963	Paenibacillus rigui JCM 16352T (97.0 %)	934:972	Phylogenetic analysis based on 16S rRNA gene sequencing affiliated strain ATCC 27380T to the genus Paenibacillus, and showed the highest sequence similarity to Paenibacillus rigui JCM 16352T (97.0 %).
30129919	9	16	theme	4,361,187	1125:1133	arg1	bases					1135:1139	4,361,187 bases	1125:1139	4,361,187 bases	1125:1139	The draft genome of ATCC 27380T had a size of 4,361,187 bases, with a G+C content of 51.0 %.
30129919	13	17	theme	=DSM	1782:1785	arg1	nov					1776:1778	nov	1776:1778	nov	1776:1778	nov. (=DSM 520T=NRRL NRS-1629T=ATCC 27380T) is proposed.
30129919	13	17	theme	=DSM	1782:1785	arg1	27380T					1812:1817	=DSM 520T=NRRL NRS-1629T=ATCC 27380T	1782:1817	=DSM 520T=NRRL NRS-1629T=ATCC 27380T	1782:1817	nov. (=DSM 520T=NRRL NRS-1629T=ATCC 27380T) is proposed.
30129919	4	18	theme	isoprenoid	555:564	arg1	acid					513:516	meso-diaminopimelic acid	493:516	meso-diaminopimelic acid	493:516	The cell wall contained meso-diaminopimelic acid as the diagnostic amino acid and the isoprenoid quinone was MK-7.
30129919	4	18	theme	isoprenoid	555:564	arg1	MK-7					578:581	the isoprenoid quinone was MK-7	551:581	the isoprenoid quinone was MK-7	551:581	The cell wall contained meso-diaminopimelic acid as the diagnostic amino acid and the isoprenoid quinone was MK-7.
30129919	7	19	theme	JCM	954:956	arg1	%					971:971	97.0 %	966:971	97.0 %	966:971	Phylogenetic analysis based on 16S rRNA gene sequencing affiliated strain ATCC 27380T to the genus Paenibacillus, and showed the highest sequence similarity to Paenibacillus rigui JCM 16352T (97.0 %).
30129919	7	19	theme	JCM	954:956	arg1	16352T					958:963	Paenibacillus rigui JCM 16352T	934:963	Paenibacillus rigui JCM 16352T (97.0 %)	934:972	Phylogenetic analysis based on 16S rRNA gene sequencing affiliated strain ATCC 27380T to the genus Paenibacillus, and showed the highest sequence similarity to Paenibacillus rigui JCM 16352T (97.0 %).
30129919	5	20	theme	polar	588:592	arg1	lipids					594:599	The polar lipids	584:599	The polar lipids present	584:607	The polar lipids present were phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol and one unknown phospholipid.
30129919	5	20	theme	polar	588:592	arg1	phosphatidylglycerol					614:633	phosphatidylglycerol	614:633	phosphatidylglycerol	614:633	The polar lipids present were phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol and one unknown phospholipid.
30129919	4	21	contain	contained	483:491	arg2	acid					542:545	the diagnostic amino acid	521:545	the diagnostic amino acid	521:545	The cell wall contained meso-diaminopimelic acid as the diagnostic amino acid and the isoprenoid quinone was MK-7.
30129919	4	21	contain	contained	483:491	arg1	wall					478:481	The cell wall	469:481	The cell wall	469:481	The cell wall contained meso-diaminopimelic acid as the diagnostic amino acid and the isoprenoid quinone was MK-7.
30129919	4	21	contain	contained	483:491	arg2	MK-7					578:581	the isoprenoid quinone was MK-7	551:581	the isoprenoid quinone was MK-7	551:581	The cell wall contained meso-diaminopimelic acid as the diagnostic amino acid and the isoprenoid quinone was MK-7.
30129919	4	21	contain	contained	483:491	arg2	acid					513:516	meso-diaminopimelic acid	493:516	meso-diaminopimelic acid	493:516	The cell wall contained meso-diaminopimelic acid as the diagnostic amino acid and the isoprenoid quinone was MK-7.
30129919	10	22	dep	in	1208:1209	arg1	silico					1211:1216	silico	1211:1216	silico	1211:1216	The average nucleotide identity and in silico DNA-DNA hybridization values between strain ATCC 27380T and P. rigui JCM 16352T were 72.5% and 18.5 %, respectively, which were below the threshold suggested for species differentiation (96% and 70 %, respectively).
30129919	2	23	attach	isolated	268:275	arg1	soil					296:299	heat-stressed soil	282:299	heat-stressed soil of Cape Canaveral, Florida, USA	282:331	A Gram-stain-positive, motile, endospore-producing, facultative anaerobic bacterial strain, designated ATCC 27380T, was isolated from heat-stressed soil of Cape Canaveral, Florida, USA.
30129919	2	23	attach	isolated	268:275	arg2	strain					232:237	A Gram-stain-positive, motile, endospore-producing, facultative anaerobic bacterial strain	148:237	A Gram-stain-positive, motile, endospore-producing, facultative anaerobic bacterial strain	148:237	A Gram-stain-positive, motile, endospore-producing, facultative anaerobic bacterial strain, designated ATCC 27380T, was isolated from heat-stressed soil of Cape Canaveral, Florida, USA.
30129919	5	24	theme	present	601:607	arg1	lipids					594:599	The polar lipids	584:599	The polar lipids present	584:607	The polar lipids present were phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol and one unknown phospholipid.
30129919	5	24	theme	present	601:607	arg1	phosphatidylglycerol					614:633	phosphatidylglycerol	614:633	phosphatidylglycerol	614:633	The polar lipids present were phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol and one unknown phospholipid.
30129919	2	25	theme	heat-stressed	282:294	arg1	soil					296:299	heat-stressed soil	282:299	heat-stressed soil of Cape Canaveral, Florida, USA	282:331	A Gram-stain-positive, motile, endospore-producing, facultative anaerobic bacterial strain, designated ATCC 27380T, was isolated from heat-stressed soil of Cape Canaveral, Florida, USA.
30129919	11	26	theme	genus	1560:1564	arg1	demarcation					1572:1582	the suggested genus level demarcation	1546:1582	the suggested genus level demarcation of 65 %	1546:1590	The average amino acid identity between strain ATCC 27380T and P. rigui JCM 16352T was 68.72 %, which was above the suggested genus level demarcation of 65 %.
30129919	9	27	theme	%	1169:1169	arg1	G+C content					1149:1159	a G+C content	1147:1159	a G+C content of 51.0 %	1147:1169	The draft genome of ATCC 27380T had a size of 4,361,187 bases, with a G+C content of 51.0 %.
30129919	7	28	theme	highest	903:909	arg1	similarity					920:929	the highest sequence similarity	899:929	the highest sequence similarity	899:929	Phylogenetic analysis based on 16S rRNA gene sequencing affiliated strain ATCC 27380T to the genus Paenibacillus, and showed the highest sequence similarity to Paenibacillus rigui JCM 16352T (97.0 %).
30129919	10	29	theme	P.	1278:1279	arg1	16352T					1291:1296	P. rigui JCM 16352T	1278:1296	P. rigui JCM 16352T	1278:1296	The average nucleotide identity and in silico DNA-DNA hybridization values between strain ATCC 27380T and P. rigui JCM 16352T were 72.5% and 18.5 %, respectively, which were below the threshold suggested for species differentiation (96% and 70 %, respectively).
30129919	1	30	theme	heat	58:61	arg1	bacterium					87:95	an extremely dry heat resistant spore forming bacterium	41:95	an extremely dry heat resistant spore forming bacterium isolated from the soil of Cape Canaveral, Florida	41:145	nov., an extremely dry heat resistant spore forming bacterium isolated from the soil of Cape Canaveral, Florida.
30129919	1	30	theme	heat	58:61	arg1	nov.					35:38	nov.	35:38	nov.	35:38	nov., an extremely dry heat resistant spore forming bacterium isolated from the soil of Cape Canaveral, Florida.
30129919	10	31	dep	threshold	1356:1364	arg1	%					1407:1407	96%	1405:1407	96%	1405:1407	The average nucleotide identity and in silico DNA-DNA hybridization values between strain ATCC 27380T and P. rigui JCM 16352T were 72.5% and 18.5 %, respectively, which were below the threshold suggested for species differentiation (96% and 70 %, respectively).
30129919	10	31	dep	threshold	1356:1364	arg1	%					1416:1416	70 %	1413:1416	70 %	1413:1416	The average nucleotide identity and in silico DNA-DNA hybridization values between strain ATCC 27380T and P. rigui JCM 16352T were 72.5% and 18.5 %, respectively, which were below the threshold suggested for species differentiation (96% and 70 %, respectively).
30129919	1	32	theme	spore	73:77	arg1	bacterium					87:95	an extremely dry heat resistant spore forming bacterium	41:95	an extremely dry heat resistant spore forming bacterium isolated from the soil of Cape Canaveral, Florida	41:145	nov., an extremely dry heat resistant spore forming bacterium isolated from the soil of Cape Canaveral, Florida.
30129919	1	32	theme	spore	73:77	arg1	nov.					35:38	nov.	35:38	nov.	35:38	nov., an extremely dry heat resistant spore forming bacterium isolated from the soil of Cape Canaveral, Florida.
30129919	0	33	theme	Paenibacillus	0:12	arg1	xerothermodurans					14:29	Paenibacillus xerothermodurans	0:29	Paenibacillus xerothermodurans	0:29	Paenibacillus xerothermodurans sp.
30129919	8	34	theme	other	979:983	arg1	strains					1006:1012	The other closely related type strains	975:1012	The other closely related type strains	975:1012	The other closely related type strains exhibited 16S rRNA gene sequence similarity values below 95.9 %.
30129919	13	35	theme	520T=NRRL	1787:1795	arg1	nov					1776:1778	nov	1776:1778	nov	1776:1778	nov. (=DSM 520T=NRRL NRS-1629T=ATCC 27380T) is proposed.
30129919	13	35	theme	520T=NRRL	1787:1795	arg1	27380T					1812:1817	=DSM 520T=NRRL NRS-1629T=ATCC 27380T	1782:1817	=DSM 520T=NRRL NRS-1629T=ATCC 27380T	1782:1817	nov. (=DSM 520T=NRRL NRS-1629T=ATCC 27380T) is proposed.
30129919	8	36	theme	related	993:999	arg1	strains					1006:1012	The other closely related type strains	975:1012	The other closely related type strains	975:1012	The other closely related type strains exhibited 16S rRNA gene sequence similarity values below 95.9 %.
30129919	10	37	theme	nucleotide	1184:1193	arg1	identity					1195:1202	average nucleotide identity	1176:1202	average nucleotide identity	1176:1202	The average nucleotide identity and in silico DNA-DNA hybridization values between strain ATCC 27380T and P. rigui JCM 16352T were 72.5% and 18.5 %, respectively, which were below the threshold suggested for species differentiation (96% and 70 %, respectively).
30129919	3	38	theme	optimum	400:406	arg1	pH					408:409	optimum pH 7.0	400:413	optimum pH 7.0	400:413	Growth was observed at 20-42 °C (optimum, 37 °C), at pH 6.0-10.0 (optimum pH 7.0) and in the presence of 0.5-3 % NaCl (optimum 0.5 %).
30129919	3	38	theme	optimum	400:406	arg1	pH					387:388	pH 6.0-10.0	387:397	pH 6.0-10.0 (optimum pH 7.0)	387:414	Growth was observed at 20-42 °C (optimum, 37 °C), at pH 6.0-10.0 (optimum pH 7.0) and in the presence of 0.5-3 % NaCl (optimum 0.5 %).
30129919	12	39	theme	chemotaxonomic	1628:1641	arg1	data					1643:1646	phenotypic, genotypic and chemotaxonomic data	1602:1646	phenotypic, genotypic and chemotaxonomic data	1602:1646	Based on phenotypic, genotypic and chemotaxonomic data, strain ATCC 27380T represents a novel species in the genus Paenibacillus, for which the name Paenibacillusxerothermodurans sp.
30129919	10	40	theme	hybridization	1226:1238	arg1	values					1240:1245	The average nucleotide identity and in silico DNA-DNA hybridization values	1172:1245	The average nucleotide identity and in silico DNA-DNA hybridization values between strain ATCC 27380T and P. rigui JCM 16352T	1172:1296	The average nucleotide identity and in silico DNA-DNA hybridization values between strain ATCC 27380T and P. rigui JCM 16352T were 72.5% and 18.5 %, respectively, which were below the threshold suggested for species differentiation (96% and 70 %, respectively).
30129919	10	40	theme	hybridization	1226:1238	arg1	%					1307:1307	72.5%	1303:1307	72.5%	1303:1307	The average nucleotide identity and in silico DNA-DNA hybridization values between strain ATCC 27380T and P. rigui JCM 16352T were 72.5% and 18.5 %, respectively, which were below the threshold suggested for species differentiation (96% and 70 %, respectively).
30129919	8	41	theme	16S	1024:1026	arg1	similarity					1047:1056	16S rRNA gene sequence similarity	1024:1056	16S rRNA gene sequence similarity values below 95.9 %	1024:1076	The other closely related type strains exhibited 16S rRNA gene sequence similarity values below 95.9 %.
30129919	10	42	theme	species	1380:1386	arg1	differentiation					1388:1402	species differentiation	1380:1402	species differentiation	1380:1402	The average nucleotide identity and in silico DNA-DNA hybridization values between strain ATCC 27380T and P. rigui JCM 16352T were 72.5% and 18.5 %, respectively, which were below the threshold suggested for species differentiation (96% and 70 %, respectively).
30129919	11	43	theme	suggested	1550:1558	arg1	demarcation					1572:1582	the suggested genus level demarcation	1546:1582	the suggested genus level demarcation of 65 %	1546:1590	The average amino acid identity between strain ATCC 27380T and P. rigui JCM 16352T was 68.72 %, which was above the suggested genus level demarcation of 65 %.
30129919	8	44	theme	gene	1033:1036	arg1	similarity					1047:1056	16S rRNA gene sequence similarity	1024:1056	16S rRNA gene sequence similarity values below 95.9 %	1024:1076	The other closely related type strains exhibited 16S rRNA gene sequence similarity values below 95.9 %.
30129919	0	45	dep	sp	31:32	arg1	xerothermodurans					14:29	Paenibacillus xerothermodurans	0:29	Paenibacillus xerothermodurans	0:29	Paenibacillus xerothermodurans sp.
30129919	12	46	theme	phenotypic	1602:1611	arg1	data					1643:1646	phenotypic, genotypic and chemotaxonomic data	1602:1646	phenotypic, genotypic and chemotaxonomic data	1602:1646	Based on phenotypic, genotypic and chemotaxonomic data, strain ATCC 27380T represents a novel species in the genus Paenibacillus, for which the name Paenibacillusxerothermodurans sp.
30129919	12	47	theme	novel	1681:1685	arg1	species					1687:1693	a novel species	1679:1693	a novel species	1679:1693	Based on phenotypic, genotypic and chemotaxonomic data, strain ATCC 27380T represents a novel species in the genus Paenibacillus, for which the name Paenibacillusxerothermodurans sp.
30129919	10	48	theme	ATCC	1262:1265	arg1	27380T					1267:1272	strain ATCC 27380T	1255:1272	strain ATCC 27380T	1255:1272	The average nucleotide identity and in silico DNA-DNA hybridization values between strain ATCC 27380T and P. rigui JCM 16352T were 72.5% and 18.5 %, respectively, which were below the threshold suggested for species differentiation (96% and 70 %, respectively).
30129919	3	49	theme	%	445:445	arg1	%					465:465	optimum 0.5 %	453:465	optimum 0.5 %	453:465	Growth was observed at 20-42 °C (optimum, 37 °C), at pH 6.0-10.0 (optimum pH 7.0) and in the presence of 0.5-3 % NaCl (optimum 0.5 %).
30129919	3	49	theme	%	445:445	arg1	 NaCl					446:450	0.5-3 % NaCl	439:450	0.5-3 % NaCl (optimum 0.5 %)	439:466	Growth was observed at 20-42 °C (optimum, 37 °C), at pH 6.0-10.0 (optimum pH 7.0) and in the presence of 0.5-3 % NaCl (optimum 0.5 %).
30129919	7	50	theme	strain	841:846	arg1	27380T					853:858	strain ATCC 27380T	841:858	strain ATCC 27380T	841:858	Phylogenetic analysis based on 16S rRNA gene sequencing affiliated strain ATCC 27380T to the genus Paenibacillus, and showed the highest sequence similarity to Paenibacillus rigui JCM 16352T (97.0 %).
30129919	8	51	theme	similarity	1047:1056	arg1	values					1058:1063	16S rRNA gene sequence similarity values	1024:1063	16S rRNA gene sequence similarity values below 95.9 %	1024:1076	The other closely related type strains exhibited 16S rRNA gene sequence similarity values below 95.9 %.
30129919	3	52	located	observed	345:352	arg2	Growth					334:339	Growth	334:339	Growth	334:339	Growth was observed at 20-42 °C (optimum, 37 °C), at pH 6.0-10.0 (optimum pH 7.0) and in the presence of 0.5-3 % NaCl (optimum 0.5 %).
30129919	3	52	located	observed	345:352	arg1	optimum					367:373	optimum	367:373	optimum	367:373	Growth was observed at 20-42 °C (optimum, 37 °C), at pH 6.0-10.0 (optimum pH 7.0) and in the presence of 0.5-3 % NaCl (optimum 0.5 %).
30129919	3	52	located	observed	345:352	arg1	20-42 °C					357:364	20-42 °C	357:364	20-42 °C (optimum, 37 °C)	357:381	Growth was observed at 20-42 °C (optimum, 37 °C), at pH 6.0-10.0 (optimum pH 7.0) and in the presence of 0.5-3 % NaCl (optimum 0.5 %).
30129919	12	53	theme	genotypic	1614:1622	arg1	data					1643:1646	phenotypic, genotypic and chemotaxonomic data	1602:1646	phenotypic, genotypic and chemotaxonomic data	1602:1646	Based on phenotypic, genotypic and chemotaxonomic data, strain ATCC 27380T represents a novel species in the genus Paenibacillus, for which the name Paenibacillusxerothermodurans sp.
30129919	4	54	theme	diagnostic	525:534	arg1	acid					542:545	the diagnostic amino acid	521:545	the diagnostic amino acid	521:545	The cell wall contained meso-diaminopimelic acid as the diagnostic amino acid and the isoprenoid quinone was MK-7.
30129919	4	54	theme	diagnostic	525:534	arg1	acid					513:516	meso-diaminopimelic acid	493:516	meso-diaminopimelic acid	493:516	The cell wall contained meso-diaminopimelic acid as the diagnostic amino acid and the isoprenoid quinone was MK-7.
30129919	11	55	theme	P.	1497:1498	arg1	16352T					1510:1515	P. rigui JCM 16352T	1497:1515	P. rigui JCM 16352T	1497:1515	The average amino acid identity between strain ATCC 27380T and P. rigui JCM 16352T was 68.72 %, which was above the suggested genus level demarcation of 65 %.
30129919	9	56	theme	draft	1083:1087	arg1	genome					1089:1094	The draft genome	1079:1094	The draft genome of ATCC 27380T	1079:1109	The draft genome of ATCC 27380T had a size of 4,361,187 bases, with a G+C content of 51.0 %.
30129919	12	57	theme	ATCC	1656:1659	arg1	27380T					1661:1666	strain ATCC 27380T	1649:1666	strain ATCC 27380T	1649:1666	Based on phenotypic, genotypic and chemotaxonomic data, strain ATCC 27380T represents a novel species in the genus Paenibacillus, for which the name Paenibacillusxerothermodurans sp.
30129919	10	58	theme	rigui	1281:1285	arg1	16352T					1291:1296	P. rigui JCM 16352T	1278:1296	P. rigui JCM 16352T	1278:1296	The average nucleotide identity and in silico DNA-DNA hybridization values between strain ATCC 27380T and P. rigui JCM 16352T were 72.5% and 18.5 %, respectively, which were below the threshold suggested for species differentiation (96% and 70 %, respectively).
30129919	3	59	theme	0.5 	461:464	arg1	%					465:465	optimum 0.5 %	453:465	optimum 0.5 %	453:465	Growth was observed at 20-42 °C (optimum, 37 °C), at pH 6.0-10.0 (optimum pH 7.0) and in the presence of 0.5-3 % NaCl (optimum 0.5 %).
30129919	3	59	theme	0.5 	461:464	arg1	 NaCl					446:450	0.5-3 % NaCl	439:450	0.5-3 % NaCl (optimum 0.5 %)	439:466	Growth was observed at 20-42 °C (optimum, 37 °C), at pH 6.0-10.0 (optimum pH 7.0) and in the presence of 0.5-3 % NaCl (optimum 0.5 %).
30129919	7	60	theme	rRNA	809:812	arg1	sequencing					819:828	16S rRNA gene sequencing	805:828	16S rRNA gene sequencing	805:828	Phylogenetic analysis based on 16S rRNA gene sequencing affiliated strain ATCC 27380T to the genus Paenibacillus, and showed the highest sequence similarity to Paenibacillus rigui JCM 16352T (97.0 %).
30129919	11	61	theme	amino	1446:1450	arg1	identity					1457:1464	The average amino acid identity	1434:1464	The average amino acid identity between strain ATCC 27380T and P. rigui JCM 16352T	1434:1515	The average amino acid identity between strain ATCC 27380T and P. rigui JCM 16352T was 68.72 %, which was above the suggested genus level demarcation of 65 %.
30129919	11	61	theme	amino	1446:1450	arg1	%					1527:1527	68.72 %	1521:1527	68.72 %	1521:1527	The average amino acid identity between strain ATCC 27380T and P. rigui JCM 16352T was 68.72 %, which was above the suggested genus level demarcation of 65 %.
30129919	4	62	theme	meso-diaminopimelic	493:511	arg1	acid					542:545	the diagnostic amino acid	521:545	the diagnostic amino acid	521:545	The cell wall contained meso-diaminopimelic acid as the diagnostic amino acid and the isoprenoid quinone was MK-7.
30129919	4	62	theme	meso-diaminopimelic	493:511	arg1	acid					513:516	meso-diaminopimelic acid	493:516	meso-diaminopimelic acid	493:516	The cell wall contained meso-diaminopimelic acid as the diagnostic amino acid and the isoprenoid quinone was MK-7.
30129919	4	62	theme	meso-diaminopimelic	493:511	arg1	MK-7					578:581	the isoprenoid quinone was MK-7	551:581	the isoprenoid quinone was MK-7	551:581	The cell wall contained meso-diaminopimelic acid as the diagnostic amino acid and the isoprenoid quinone was MK-7.
30129919	9	63	theme	27380T	1104:1109	arg1	genome					1089:1094	The draft genome	1079:1094	The draft genome of ATCC 27380T	1079:1109	The draft genome of ATCC 27380T had a size of 4,361,187 bases, with a G+C content of 51.0 %.
30129919	4	64	theme	quinone	566:572	arg1	acid					513:516	meso-diaminopimelic acid	493:516	meso-diaminopimelic acid	493:516	The cell wall contained meso-diaminopimelic acid as the diagnostic amino acid and the isoprenoid quinone was MK-7.
30129919	4	64	theme	quinone	566:572	arg1	MK-7					578:581	the isoprenoid quinone was MK-7	551:581	the isoprenoid quinone was MK-7	551:581	The cell wall contained meso-diaminopimelic acid as the diagnostic amino acid and the isoprenoid quinone was MK-7.
30129919	11	65	theme	JCM	1506:1508	arg1	16352T					1510:1515	P. rigui JCM 16352T	1497:1515	P. rigui JCM 16352T	1497:1515	The average amino acid identity between strain ATCC 27380T and P. rigui JCM 16352T was 68.72 %, which was above the suggested genus level demarcation of 65 %.
30129919	2	66	theme	Canaveral	309:317	arg1	soil					296:299	heat-stressed soil	282:299	heat-stressed soil of Cape Canaveral, Florida, USA	282:331	A Gram-stain-positive, motile, endospore-producing, facultative anaerobic bacterial strain, designated ATCC 27380T, was isolated from heat-stressed soil of Cape Canaveral, Florida, USA.
30129919	12	67	theme	strain	1649:1654	arg1	27380T					1661:1666	strain ATCC 27380T	1649:1666	strain ATCC 27380T	1649:1666	Based on phenotypic, genotypic and chemotaxonomic data, strain ATCC 27380T represents a novel species in the genus Paenibacillus, for which the name Paenibacillusxerothermodurans sp.
30129919	6	68	theme	main	719:722	arg1	acids					730:734	The main fatty acids	715:734	The main fatty acids	715:734	The main fatty acids were iso-C15 : 0 and anteiso-C15 : 0.
30129919	6	68	theme	main	719:722	arg1	iso-C15 					741:748	iso-C15 	741:748	iso-C15 	741:748	The main fatty acids were iso-C15 : 0 and anteiso-C15 : 0.
30129919	9	69	theme	bases	1135:1139	arg1	size					1117:1120	a size	1115:1120	a size of 4,361,187 bases	1115:1139	The draft genome of ATCC 27380T had a size of 4,361,187 bases, with a G+C content of 51.0 %.
30129919	12	70	theme	Paenibacillusxerothermodurans	1742:1770	arg1	sp					1772:1773	the name Paenibacillusxerothermodurans sp	1733:1773	the name Paenibacillusxerothermodurans sp	1733:1773	Based on phenotypic, genotypic and chemotaxonomic data, strain ATCC 27380T represents a novel species in the genus Paenibacillus, for which the name Paenibacillusxerothermodurans sp.
30129919	2	71	theme	bacterial	222:230	arg1	strain					232:237	A Gram-stain-positive, motile, endospore-producing, facultative anaerobic bacterial strain	148:237	A Gram-stain-positive, motile, endospore-producing, facultative anaerobic bacterial strain	148:237	A Gram-stain-positive, motile, endospore-producing, facultative anaerobic bacterial strain, designated ATCC 27380T, was isolated from heat-stressed soil of Cape Canaveral, Florida, USA.
30129919	1	72	attach	isolated	97:104	arg2	bacterium					87:95	an extremely dry heat resistant spore forming bacterium	41:95	an extremely dry heat resistant spore forming bacterium isolated from the soil of Cape Canaveral, Florida	41:145	nov., an extremely dry heat resistant spore forming bacterium isolated from the soil of Cape Canaveral, Florida.
30129919	1	72	attach	isolated	97:104	arg1	soil					115:118	the soil	111:118	the soil of Cape Canaveral, Florida	111:145	nov., an extremely dry heat resistant spore forming bacterium isolated from the soil of Cape Canaveral, Florida.
30129919	1	72	attach	isolated	97:104	arg2	nov.					35:38	nov.	35:38	nov.	35:38	nov., an extremely dry heat resistant spore forming bacterium isolated from the soil of Cape Canaveral, Florida.
30129919	7	73	theme	rigui	948:952	arg1	%					971:971	97.0 %	966:971	97.0 %	966:971	Phylogenetic analysis based on 16S rRNA gene sequencing affiliated strain ATCC 27380T to the genus Paenibacillus, and showed the highest sequence similarity to Paenibacillus rigui JCM 16352T (97.0 %).
30129919	7	73	theme	rigui	948:952	arg1	16352T					958:963	Paenibacillus rigui JCM 16352T	934:963	Paenibacillus rigui JCM 16352T (97.0 %)	934:972	Phylogenetic analysis based on 16S rRNA gene sequencing affiliated strain ATCC 27380T to the genus Paenibacillus, and showed the highest sequence similarity to Paenibacillus rigui JCM 16352T (97.0 %).
30129919	5	74	theme	unknown	693:699	arg1	phospholipid					701:712	one unknown phospholipid	689:712	one unknown phospholipid	689:712	The polar lipids present were phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol and one unknown phospholipid.
30129919	2	75	theme	ATCC	251:254	arg1	27380T					256:261	ATCC 27380T	251:261	ATCC 27380T	251:261	A Gram-stain-positive, motile, endospore-producing, facultative anaerobic bacterial strain, designated ATCC 27380T, was isolated from heat-stressed soil of Cape Canaveral, Florida, USA.
30129919	1	76	theme	Canaveral	128:136	arg1	soil					115:118	the soil	111:118	the soil of Cape Canaveral, Florida	111:145	nov., an extremely dry heat resistant spore forming bacterium isolated from the soil of Cape Canaveral, Florida.
30129919	11	77	theme	level	1566:1570	arg1	demarcation					1572:1582	the suggested genus level demarcation	1546:1582	the suggested genus level demarcation of 65 %	1546:1590	The average amino acid identity between strain ATCC 27380T and P. rigui JCM 16352T was 68.72 %, which was above the suggested genus level demarcation of 65 %.
30129919	7	78	theme	sequence	911:918	arg1	similarity					920:929	the highest sequence similarity	899:929	the highest sequence similarity	899:929	Phylogenetic analysis based on 16S rRNA gene sequencing affiliated strain ATCC 27380T to the genus Paenibacillus, and showed the highest sequence similarity to Paenibacillus rigui JCM 16352T (97.0 %).
30129919	12	79	theme	name	1737:1740	arg1	sp					1772:1773	the name Paenibacillusxerothermodurans sp	1733:1773	the name Paenibacillusxerothermodurans sp	1733:1773	Based on phenotypic, genotypic and chemotaxonomic data, strain ATCC 27380T represents a novel species in the genus Paenibacillus, for which the name Paenibacillusxerothermodurans sp.
30129919	1	80	theme	dry	54:56	arg1	bacterium					87:95	an extremely dry heat resistant spore forming bacterium	41:95	an extremely dry heat resistant spore forming bacterium isolated from the soil of Cape Canaveral, Florida	41:145	nov., an extremely dry heat resistant spore forming bacterium isolated from the soil of Cape Canaveral, Florida.
30129919	1	80	theme	dry	54:56	arg1	nov.					35:38	nov.	35:38	nov.	35:38	nov., an extremely dry heat resistant spore forming bacterium isolated from the soil of Cape Canaveral, Florida.
30129919	3	81	dep	optimum	367:373	arg1	37 °C					376:380	37 °C	376:380	37 °C	376:380	Growth was observed at 20-42 °C (optimum, 37 °C), at pH 6.0-10.0 (optimum pH 7.0) and in the presence of 0.5-3 % NaCl (optimum 0.5 %).
30129919	10	82	theme	average	1176:1182	arg1	identity					1195:1202	average nucleotide identity	1176:1202	average nucleotide identity	1176:1202	The average nucleotide identity and in silico DNA-DNA hybridization values between strain ATCC 27380T and P. rigui JCM 16352T were 72.5% and 18.5 %, respectively, which were below the threshold suggested for species differentiation (96% and 70 %, respectively).
30129919	11	83	theme	%	1590:1590	arg1	demarcation					1572:1582	the suggested genus level demarcation	1546:1582	the suggested genus level demarcation of 65 %	1546:1590	The average amino acid identity between strain ATCC 27380T and P. rigui JCM 16352T was 68.72 %, which was above the suggested genus level demarcation of 65 %.
30129919	1	84	theme	resistant	63:71	arg1	bacterium					87:95	an extremely dry heat resistant spore forming bacterium	41:95	an extremely dry heat resistant spore forming bacterium isolated from the soil of Cape Canaveral, Florida	41:145	nov., an extremely dry heat resistant spore forming bacterium isolated from the soil of Cape Canaveral, Florida.
30129919	1	84	theme	resistant	63:71	arg1	nov.					35:38	nov.	35:38	nov.	35:38	nov., an extremely dry heat resistant spore forming bacterium isolated from the soil of Cape Canaveral, Florida.
30129919	7	85	theme	Paenibacillus	873:885	arg1	genus					867:871	the genus Paenibacillus	863:885	the genus Paenibacillus	863:885	Phylogenetic analysis based on 16S rRNA gene sequencing affiliated strain ATCC 27380T to the genus Paenibacillus, and showed the highest sequence similarity to Paenibacillus rigui JCM 16352T (97.0 %).
30129919	1	86	theme	forming	79:85	arg1	bacterium					87:95	an extremely dry heat resistant spore forming bacterium	41:95	an extremely dry heat resistant spore forming bacterium isolated from the soil of Cape Canaveral, Florida	41:145	nov., an extremely dry heat resistant spore forming bacterium isolated from the soil of Cape Canaveral, Florida.
30129919	1	86	theme	forming	79:85	arg1	nov.					35:38	nov.	35:38	nov.	35:38	nov., an extremely dry heat resistant spore forming bacterium isolated from the soil of Cape Canaveral, Florida.
30129919	10	87	theme	in	1208:1209	arg1	hybridization					1226:1238	in silico DNA-DNA hybridization	1208:1238	in silico DNA-DNA hybridization	1208:1238	The average nucleotide identity and in silico DNA-DNA hybridization values between strain ATCC 27380T and P. rigui JCM 16352T were 72.5% and 18.5 %, respectively, which were below the threshold suggested for species differentiation (96% and 70 %, respectively).
30129919	8	88	theme	type	1001:1004	arg1	strains					1006:1012	The other closely related type strains	975:1012	The other closely related type strains	975:1012	The other closely related type strains exhibited 16S rRNA gene sequence similarity values below 95.9 %.
30129919	11	89	theme	strain	1474:1479	arg1	27380T					1486:1491	strain ATCC 27380T	1474:1491	strain ATCC 27380T	1474:1491	The average amino acid identity between strain ATCC 27380T and P. rigui JCM 16352T was 68.72 %, which was above the suggested genus level demarcation of 65 %.
30129919	4	90	theme	cell	473:476	arg1	wall					478:481	The cell wall	469:481	The cell wall	469:481	The cell wall contained meso-diaminopimelic acid as the diagnostic amino acid and the isoprenoid quinone was MK-7.
30129919	10	91	theme	identity	1195:1202	arg1	values					1240:1245	The average nucleotide identity and in silico DNA-DNA hybridization values	1172:1245	The average nucleotide identity and in silico DNA-DNA hybridization values between strain ATCC 27380T and P. rigui JCM 16352T	1172:1296	The average nucleotide identity and in silico DNA-DNA hybridization values between strain ATCC 27380T and P. rigui JCM 16352T were 72.5% and 18.5 %, respectively, which were below the threshold suggested for species differentiation (96% and 70 %, respectively).
30129919	10	91	theme	identity	1195:1202	arg1	%					1307:1307	72.5%	1303:1307	72.5%	1303:1307	The average nucleotide identity and in silico DNA-DNA hybridization values between strain ATCC 27380T and P. rigui JCM 16352T were 72.5% and 18.5 %, respectively, which were below the threshold suggested for species differentiation (96% and 70 %, respectively).
30129919	2	92	theme	Gram-stain-positive	150:168	arg1	strain					232:237	A Gram-stain-positive, motile, endospore-producing, facultative anaerobic bacterial strain	148:237	A Gram-stain-positive, motile, endospore-producing, facultative anaerobic bacterial strain	148:237	A Gram-stain-positive, motile, endospore-producing, facultative anaerobic bacterial strain, designated ATCC 27380T, was isolated from heat-stressed soil of Cape Canaveral, Florida, USA.
30129919	11	93	theme	average	1438:1444	arg1	identity					1457:1464	The average amino acid identity	1434:1464	The average amino acid identity between strain ATCC 27380T and P. rigui JCM 16352T	1434:1515	The average amino acid identity between strain ATCC 27380T and P. rigui JCM 16352T was 68.72 %, which was above the suggested genus level demarcation of 65 %.
30129919	11	93	theme	average	1438:1444	arg1	%					1527:1527	68.72 %	1521:1527	68.72 %	1521:1527	The average amino acid identity between strain ATCC 27380T and P. rigui JCM 16352T was 68.72 %, which was above the suggested genus level demarcation of 65 %.
30129919	8	94	theme	rRNA	1028:1031	arg1	similarity					1047:1056	16S rRNA gene sequence similarity	1024:1056	16S rRNA gene sequence similarity values below 95.9 %	1024:1076	The other closely related type strains exhibited 16S rRNA gene sequence similarity values below 95.9 %.
30129919	10	95	theme	DNA-DNA	1218:1224	arg1	hybridization					1226:1238	in silico DNA-DNA hybridization	1208:1238	in silico DNA-DNA hybridization	1208:1238	The average nucleotide identity and in silico DNA-DNA hybridization values between strain ATCC 27380T and P. rigui JCM 16352T were 72.5% and 18.5 %, respectively, which were below the threshold suggested for species differentiation (96% and 70 %, respectively).
30129919	7	96	theme	ATCC	848:851	arg1	27380T					853:858	strain ATCC 27380T	841:858	strain ATCC 27380T	841:858	Phylogenetic analysis based on 16S rRNA gene sequencing affiliated strain ATCC 27380T to the genus Paenibacillus, and showed the highest sequence similarity to Paenibacillus rigui JCM 16352T (97.0 %).
30129919	8	97	theme	sequence	1038:1045	arg1	similarity					1047:1056	16S rRNA gene sequence similarity	1024:1056	16S rRNA gene sequence similarity values below 95.9 %	1024:1076	The other closely related type strains exhibited 16S rRNA gene sequence similarity values below 95.9 %.
30129919	10	98	theme	strain	1255:1260	arg1	27380T					1267:1272	strain ATCC 27380T	1255:1272	strain ATCC 27380T	1255:1272	The average nucleotide identity and in silico DNA-DNA hybridization values between strain ATCC 27380T and P. rigui JCM 16352T were 72.5% and 18.5 %, respectively, which were below the threshold suggested for species differentiation (96% and 70 %, respectively).
30129919	13	99	theme	NRS-1629T=ATCC	1797:1810	arg1	nov					1776:1778	nov	1776:1778	nov	1776:1778	nov. (=DSM 520T=NRRL NRS-1629T=ATCC 27380T) is proposed.
30129919	13	99	theme	NRS-1629T=ATCC	1797:1810	arg1	27380T					1812:1817	=DSM 520T=NRRL NRS-1629T=ATCC 27380T	1782:1817	=DSM 520T=NRRL NRS-1629T=ATCC 27380T	1782:1817	nov. (=DSM 520T=NRRL NRS-1629T=ATCC 27380T) is proposed.
30129919	10	100	dep	%	1307:1307	arg1	threshold					1356:1364	the threshold	1352:1364	the threshold suggested for species differentiation	1352:1402	The average nucleotide identity and in silico DNA-DNA hybridization values between strain ATCC 27380T and P. rigui JCM 16352T were 72.5% and 18.5 %, respectively, which were below the threshold suggested for species differentiation (96% and 70 %, respectively).
30129919	4	101	theme	amino	536:540	arg1	acid					542:545	the diagnostic amino acid	521:545	the diagnostic amino acid	521:545	The cell wall contained meso-diaminopimelic acid as the diagnostic amino acid and the isoprenoid quinone was MK-7.
30129919	4	101	theme	amino	536:540	arg1	acid					513:516	meso-diaminopimelic acid	493:516	meso-diaminopimelic acid	493:516	The cell wall contained meso-diaminopimelic acid as the diagnostic amino acid and the isoprenoid quinone was MK-7.
30129919	11	102	theme	acid	1452:1455	arg1	identity					1457:1464	The average amino acid identity	1434:1464	The average amino acid identity between strain ATCC 27380T and P. rigui JCM 16352T	1434:1515	The average amino acid identity between strain ATCC 27380T and P. rigui JCM 16352T was 68.72 %, which was above the suggested genus level demarcation of 65 %.
30129919	11	102	theme	acid	1452:1455	arg1	%					1527:1527	68.72 %	1521:1527	68.72 %	1521:1527	The average amino acid identity between strain ATCC 27380T and P. rigui JCM 16352T was 68.72 %, which was above the suggested genus level demarcation of 65 %.
30129919	3	103	theme	 NaCl	446:450	arg1	presence					427:434	the presence	423:434	the presence of 0.5-3 % NaCl (optimum 0.5 %)	423:466	Growth was observed at 20-42 °C (optimum, 37 °C), at pH 6.0-10.0 (optimum pH 7.0) and in the presence of 0.5-3 % NaCl (optimum 0.5 %).
30129919	7	104	theme	gene	814:817	arg1	sequencing					819:828	16S rRNA gene sequencing	805:828	16S rRNA gene sequencing	805:828	Phylogenetic analysis based on 16S rRNA gene sequencing affiliated strain ATCC 27380T to the genus Paenibacillus, and showed the highest sequence similarity to Paenibacillus rigui JCM 16352T (97.0 %).
29801845	8	0	theme	excellent	979:987	arg1	protection					989:998	an excellent protection	976:998	an excellent protection of fish fillet	976:1013	Ps-bones incorporated during fillet cooking offered an excellent protection of fish fillet by avoiding pH change, color loss, lipid oxidation and spoilage.
29801845	2	1	theme	total	337:341	arg1	sugar					343:347	total sugar	337:347	total sugar	337:347	Results of chemical characterization revealed that Ps-bones showed the highest total sugar, uronic acid and sulfate group contents.
29801845	4	2	theme	sulfate	561:567	arg1	groups					569:574	sulfate groups	561:574	sulfate groups	561:574	The presence of sulfate groups in different Ps was confirmed by FTIR analysis.
29801845	2	3	theme	highest	329:335	arg1	sugar					343:347	total sugar	337:347	total sugar	337:347	Results of chemical characterization revealed that Ps-bones showed the highest total sugar, uronic acid and sulfate group contents.
29801845	8	4	theme	fillet	1008:1013	arg1	protection					989:998	an excellent protection	976:998	an excellent protection of fish fillet	976:1013	Ps-bones incorporated during fillet cooking offered an excellent protection of fish fillet by avoiding pH change, color loss, lipid oxidation and spoilage.
29801845	1	5	from	isolation	158:166	arg1	by-products					222:232	the Bullet tuna by-products	206:232	the Bullet tuna by-products (head, skin and bones)	206:255	The present study deals with the isolation of sulfated polysaccharides (Ps) from the Bullet tuna by-products (head, skin and bones).
29801845	1	5	from	isolation	158:166	arg1	bones					250:254	bones	250:254	bones	250:254	The present study deals with the isolation of sulfated polysaccharides (Ps) from the Bullet tuna by-products (head, skin and bones).
29801845	1	5	from	isolation	158:166	arg1	skin					241:244	skin	241:244	skin	241:244	The present study deals with the isolation of sulfated polysaccharides (Ps) from the Bullet tuna by-products (head, skin and bones).
29801845	1	5	from	isolation	158:166	arg1	head					235:238	head	235:238	head	235:238	The present study deals with the isolation of sulfated polysaccharides (Ps) from the Bullet tuna by-products (head, skin and bones).
29801845	3	6	theme	different	516:524	arg1	weights					536:542	different molecular weights	516:542	different molecular weights	516:542	Tuna extracted-Ps contained a mixture of neutral sugars, with high amounts of glucuronic and galacturonic acids and presented different molecular weights.
29801845	3	7	theme	molecular	526:534	arg1	weights					536:542	different molecular weights	516:542	different molecular weights	516:542	Tuna extracted-Ps contained a mixture of neutral sugars, with high amounts of glucuronic and galacturonic acids and presented different molecular weights.
29801845	6	8	theme	antibacterial	797:809	arg1	activity					811:818	antibacterial activity	797:818	antibacterial activity	797:818	Moreover, results revealed that all polysaccharides displayed varying degrees of antibacterial activity.
29801845	3	9	theme	glucuronic	468:477	arg1	acids					496:500	glucuronic and galacturonic acids	468:500	glucuronic and galacturonic acids	468:500	Tuna extracted-Ps contained a mixture of neutral sugars, with high amounts of glucuronic and galacturonic acids and presented different molecular weights.
29801845	4	10	theme	groups	569:574	arg1	presence					549:556	The presence	545:556	The presence of sulfate groups in different Ps	545:590	The presence of sulfate groups in different Ps was confirmed by FTIR analysis.
29801845	8	11	theme	lipid	1050:1054	arg1	oxidation					1056:1064	lipid oxidation	1050:1064	lipid oxidation	1050:1064	Ps-bones incorporated during fillet cooking offered an excellent protection of fish fillet by avoiding pH change, color loss, lipid oxidation and spoilage.
29801845	4	12	from	presence	549:556	arg1	Ps					589:590	different Ps	579:590	different Ps	579:590	The presence of sulfate groups in different Ps was confirmed by FTIR analysis.
29801845	7	13	theme	activities	866:875	arg1	spectrum					854:861	high and wide spectrum	840:861	spectrum	854:861	Ps-bones exhibited high and wide spectrum of activities, inhibiting the growth of all bacteria tested.
29801845	2	14	theme	sulfate	366:372	arg1	contents					380:387	sulfate group contents	366:387	sulfate group contents	366:387	Results of chemical characterization revealed that Ps-bones showed the highest total sugar, uronic acid and sulfate group contents.
29801845	3	15	contain	contained	408:416	arg1	extracted-Ps					395:406	Tuna extracted-Ps	390:406	Tuna extracted-Ps	390:406	Tuna extracted-Ps contained a mixture of neutral sugars, with high amounts of glucuronic and galacturonic acids and presented different molecular weights.
29801845	3	15	contain	contained	408:416	arg2	mixture					420:426	a mixture	418:426	a mixture	418:426	Tuna extracted-Ps contained a mixture of neutral sugars, with high amounts of glucuronic and galacturonic acids and presented different molecular weights.
29801845	4	16	attach	presence	549:556	arg1	Ps					589:590	different Ps	579:590	different Ps	579:590	The presence of sulfate groups in different Ps was confirmed by FTIR analysis.
29801845	4	16	attach	presence	549:556	arg2	groups					569:574	sulfate groups	561:574	sulfate groups	561:574	The presence of sulfate groups in different Ps was confirmed by FTIR analysis.
29801845	7	17	theme	wide	849:852	arg1	spectrum					854:861	high and wide spectrum	840:861	spectrum	854:861	Ps-bones exhibited high and wide spectrum of activities, inhibiting the growth of all bacteria tested.
29801845	1	18	theme	sulfated	171:178	arg1	polysaccharides					180:194	sulfated polysaccharides	171:194	sulfated polysaccharides (Ps) from the Bullet tuna by-products (head, skin and bones)	171:255	The present study deals with the isolation of sulfated polysaccharides (Ps) from the Bullet tuna by-products (head, skin and bones).
29801845	1	18	theme	sulfated	171:178	arg1	Ps					197:198	Ps	197:198	Ps	197:198	The present study deals with the isolation of sulfated polysaccharides (Ps) from the Bullet tuna by-products (head, skin and bones).
29801845	0	19	theme	potential	10:18	arg1	Rochei					104:109	Rochei	104:109	Rochei	104:109	Bioactive potential and structural characterization of sulfated polysaccharides from Bullet tuna (Auxis Rochei) by-products.
29801845	0	19	theme	potential	10:18	arg1	by-products					112:122	Bioactive potential and structural characterization of sulfated polysaccharides from Bullet tuna (Auxis Rochei) by-products	0:122	Bioactive potential and structural characterization of sulfated polysaccharides from Bullet tuna (Auxis Rochei) by-products.	0:123	Bioactive potential and structural characterization of sulfated polysaccharides from Bullet tuna (Auxis Rochei) by-products.
29801845	8	20	theme	color	1038:1042	arg1	loss					1044:1047	color loss	1038:1047	color loss	1038:1047	Ps-bones incorporated during fillet cooking offered an excellent protection of fish fillet by avoiding pH change, color loss, lipid oxidation and spoilage.
29801845	6	21	theme	varying	778:784	arg1	degrees					786:792	varying degrees	778:792	varying degrees of antibacterial activity	778:818	Moreover, results revealed that all polysaccharides displayed varying degrees of antibacterial activity.
29801845	1	22	theme	polysaccharides	180:194	arg1	isolation					158:166	the isolation	154:166	the isolation of sulfated polysaccharides (Ps) from the Bullet tuna by-products (head, skin and bones)	154:255	The present study deals with the isolation of sulfated polysaccharides (Ps) from the Bullet tuna by-products (head, skin and bones).
29801845	0	23	theme	Bioactive	0:8	arg1	potential					10:18	Bioactive potential	0:18	Bioactive potential	0:18	Bioactive potential and structural characterization of sulfated polysaccharides from Bullet tuna (Auxis Rochei) by-products.
29801845	9	24	theme	antibacterial	1175:1187	arg1	agents					1189:1194	antibacterial agents	1175:1194	antibacterial agents	1175:1194	Overall, the results demonstrated that Ps could be potentially used as natural antioxidant and antibacterial agents.
29801845	9	24	theme	antibacterial	1175:1187	arg1	Ps					1119:1120	Ps	1119:1120	Ps	1119:1120	Overall, the results demonstrated that Ps could be potentially used as natural antioxidant and antibacterial agents.
29801845	8	25	theme	pH	1027:1028	arg1	change					1030:1035	pH change	1027:1035	pH change	1027:1035	Ps-bones incorporated during fillet cooking offered an excellent protection of fish fillet by avoiding pH change, color loss, lipid oxidation and spoilage.
29801845	2	26	theme	characterization	278:293	arg1	Results					258:264	Results	258:264	Results of chemical characterization	258:293	Results of chemical characterization revealed that Ps-bones showed the highest total sugar, uronic acid and sulfate group contents.
29801845	7	27	theme	high	840:843	arg1	spectrum					854:861	high and wide spectrum	840:861	spectrum	854:861	Ps-bones exhibited high and wide spectrum of activities, inhibiting the growth of all bacteria tested.
29801845	2	28	theme	uronic	350:355	arg1	acid					357:360	uronic acid	350:360	uronic acid	350:360	Results of chemical characterization revealed that Ps-bones showed the highest total sugar, uronic acid and sulfate group contents.
29801845	0	29	theme	structural	24:33	arg1	characterization					35:50	structural characterization	24:50	structural characterization	24:50	Bioactive potential and structural characterization of sulfated polysaccharides from Bullet tuna (Auxis Rochei) by-products.
29801845	2	30	theme	chemical	269:276	arg1	characterization					278:293	chemical characterization	269:293	chemical characterization	269:293	Results of chemical characterization revealed that Ps-bones showed the highest total sugar, uronic acid and sulfate group contents.
29801845	3	31	theme	sugars	439:444	arg1	mixture					420:426	a mixture	418:426	a mixture	418:426	Tuna extracted-Ps contained a mixture of neutral sugars, with high amounts of glucuronic and galacturonic acids and presented different molecular weights.
29801845	8	32	theme	fillet	953:958	arg1	cooking					960:966	fillet cooking	953:966	fillet cooking	953:966	Ps-bones incorporated during fillet cooking offered an excellent protection of fish fillet by avoiding pH change, color loss, lipid oxidation and spoilage.
29801845	1	33	dep	by-products	222:232	arg1	by-products					222:232	the Bullet tuna by-products	206:232	the Bullet tuna by-products (head, skin and bones)	206:255	The present study deals with the isolation of sulfated polysaccharides (Ps) from the Bullet tuna by-products (head, skin and bones).
29801845	1	33	dep	by-products	222:232	arg1	bones					250:254	bones	250:254	bones	250:254	The present study deals with the isolation of sulfated polysaccharides (Ps) from the Bullet tuna by-products (head, skin and bones).
29801845	1	33	dep	by-products	222:232	arg1	skin					241:244	skin	241:244	skin	241:244	The present study deals with the isolation of sulfated polysaccharides (Ps) from the Bullet tuna by-products (head, skin and bones).
29801845	1	33	dep	by-products	222:232	arg1	head					235:238	head	235:238	head	235:238	The present study deals with the isolation of sulfated polysaccharides (Ps) from the Bullet tuna by-products (head, skin and bones).
29801845	0	34	from	tuna	92:95	arg1	Rochei					104:109	Rochei	104:109	Rochei	104:109	Bioactive potential and structural characterization of sulfated polysaccharides from Bullet tuna (Auxis Rochei) by-products.
29801845	0	34	from	tuna	92:95	arg1	polysaccharides					64:78	sulfated polysaccharides	55:78	sulfated polysaccharides from Bullet tuna	55:95	Bioactive potential and structural characterization of sulfated polysaccharides from Bullet tuna (Auxis Rochei) by-products.
29801845	0	34	from	tuna	92:95	arg1	by-products					112:122	Bioactive potential and structural characterization of sulfated polysaccharides from Bullet tuna (Auxis Rochei) by-products	0:122	Bioactive potential and structural characterization of sulfated polysaccharides from Bullet tuna (Auxis Rochei) by-products.	0:123	Bioactive potential and structural characterization of sulfated polysaccharides from Bullet tuna (Auxis Rochei) by-products.
29801845	5	35	theme	extracted	702:710	arg1	Ps					712:713	all the extracted Ps	694:713	all the extracted Ps	694:713	Interestingly, Ps-bones showed the highest antioxidant activity among all the extracted Ps.
29801845	0	36	theme	characterization	35:50	arg1	Rochei					104:109	Rochei	104:109	Rochei	104:109	Bioactive potential and structural characterization of sulfated polysaccharides from Bullet tuna (Auxis Rochei) by-products.
29801845	0	36	theme	characterization	35:50	arg1	by-products					112:122	Bioactive potential and structural characterization of sulfated polysaccharides from Bullet tuna (Auxis Rochei) by-products	0:122	Bioactive potential and structural characterization of sulfated polysaccharides from Bullet tuna (Auxis Rochei) by-products.	0:123	Bioactive potential and structural characterization of sulfated polysaccharides from Bullet tuna (Auxis Rochei) by-products.
29801845	8	37	theme	fish	1003:1006	arg1	fillet					1008:1013	fish fillet	1003:1013	fish fillet	1003:1013	Ps-bones incorporated during fillet cooking offered an excellent protection of fish fillet by avoiding pH change, color loss, lipid oxidation and spoilage.
29801845	3	38	theme	Tuna	390:393	arg1	extracted-Ps					395:406	Tuna extracted-Ps	390:406	Tuna extracted-Ps	390:406	Tuna extracted-Ps contained a mixture of neutral sugars, with high amounts of glucuronic and galacturonic acids and presented different molecular weights.
29801845	2	39	theme	group	374:378	arg1	contents					380:387	sulfate group contents	366:387	sulfate group contents	366:387	Results of chemical characterization revealed that Ps-bones showed the highest total sugar, uronic acid and sulfate group contents.
29801845	0	40	theme	polysaccharides	64:78	arg1	Rochei					104:109	Rochei	104:109	Rochei	104:109	Bioactive potential and structural characterization of sulfated polysaccharides from Bullet tuna (Auxis Rochei) by-products.
29801845	0	40	theme	polysaccharides	64:78	arg1	by-products					112:122	Bioactive potential and structural characterization of sulfated polysaccharides from Bullet tuna (Auxis Rochei) by-products	0:122	Bioactive potential and structural characterization of sulfated polysaccharides from Bullet tuna (Auxis Rochei) by-products.	0:123	Bioactive potential and structural characterization of sulfated polysaccharides from Bullet tuna (Auxis Rochei) by-products.
29801845	1	41	from	by-products	222:232	arg1	polysaccharides					180:194	sulfated polysaccharides	171:194	sulfated polysaccharides (Ps) from the Bullet tuna by-products (head, skin and bones)	171:255	The present study deals with the isolation of sulfated polysaccharides (Ps) from the Bullet tuna by-products (head, skin and bones).
29801845	1	41	from	by-products	222:232	arg1	Ps					197:198	Ps	197:198	Ps	197:198	The present study deals with the isolation of sulfated polysaccharides (Ps) from the Bullet tuna by-products (head, skin and bones).
29801845	1	41	from	by-products	222:232	arg1	isolation					158:166	the isolation	154:166	the isolation of sulfated polysaccharides (Ps) from the Bullet tuna by-products (head, skin and bones)	154:255	The present study deals with the isolation of sulfated polysaccharides (Ps) from the Bullet tuna by-products (head, skin and bones).
29801845	0	42	theme	sulfated	55:62	arg1	polysaccharides					64:78	sulfated polysaccharides	55:78	sulfated polysaccharides from Bullet tuna	55:95	Bioactive potential and structural characterization of sulfated polysaccharides from Bullet tuna (Auxis Rochei) by-products.
29801845	5	43	theme	highest	659:665	arg1	activity					679:686	the highest antioxidant activity	655:686	the highest antioxidant activity among all the extracted Ps	655:713	Interestingly, Ps-bones showed the highest antioxidant activity among all the extracted Ps.
29801845	0	44	from	by-products	112:122	arg1	tuna					92:95	Bullet tuna	85:95	Bullet tuna	85:95	Bioactive potential and structural characterization of sulfated polysaccharides from Bullet tuna (Auxis Rochei) by-products.
29801845	9	45	used	used	1143:1146	arg2	antioxidant					1159:1169	natural antioxidant	1151:1169	natural antioxidant	1151:1169	Overall, the results demonstrated that Ps could be potentially used as natural antioxidant and antibacterial agents.
29801845	9	45	used	used	1143:1146	arg2	Ps					1119:1120	Ps	1119:1120	Ps	1119:1120	Overall, the results demonstrated that Ps could be potentially used as natural antioxidant and antibacterial agents.
29801845	9	45	used	used	1143:1146	arg2	agents					1189:1194	antibacterial agents	1175:1194	antibacterial agents	1175:1194	Overall, the results demonstrated that Ps could be potentially used as natural antioxidant and antibacterial agents.
29801845	7	46	theme	bacteria	907:914	arg1	growth					893:898	the growth	889:898	the growth of all bacteria tested	889:921	Ps-bones exhibited high and wide spectrum of activities, inhibiting the growth of all bacteria tested.
29801845	5	47	theme	antioxidant	667:677	arg1	activity					679:686	the highest antioxidant activity	655:686	the highest antioxidant activity among all the extracted Ps	655:713	Interestingly, Ps-bones showed the highest antioxidant activity among all the extracted Ps.
29801845	6	48	theme	activity	811:818	arg1	degrees					786:792	varying degrees	778:792	varying degrees of antibacterial activity	778:818	Moreover, results revealed that all polysaccharides displayed varying degrees of antibacterial activity.
29801845	1	49	theme	present	129:135	arg1	study					137:141	The present study	125:141	The present study	125:141	The present study deals with the isolation of sulfated polysaccharides (Ps) from the Bullet tuna by-products (head, skin and bones).
29801845	9	50	theme	natural	1151:1157	arg1	Ps					1119:1120	Ps	1119:1120	Ps	1119:1120	Overall, the results demonstrated that Ps could be potentially used as natural antioxidant and antibacterial agents.
29801845	9	50	theme	natural	1151:1157	arg1	antioxidant					1159:1169	natural antioxidant	1151:1169	natural antioxidant	1151:1169	Overall, the results demonstrated that Ps could be potentially used as natural antioxidant and antibacterial agents.
29801845	1	51	theme	Bullet	210:215	arg1	by-products					222:232	the Bullet tuna by-products	206:232	the Bullet tuna by-products (head, skin and bones)	206:255	The present study deals with the isolation of sulfated polysaccharides (Ps) from the Bullet tuna by-products (head, skin and bones).
29801845	1	51	theme	Bullet	210:215	arg1	bones					250:254	bones	250:254	bones	250:254	The present study deals with the isolation of sulfated polysaccharides (Ps) from the Bullet tuna by-products (head, skin and bones).
29801845	1	51	theme	Bullet	210:215	arg1	skin					241:244	skin	241:244	skin	241:244	The present study deals with the isolation of sulfated polysaccharides (Ps) from the Bullet tuna by-products (head, skin and bones).
29801845	1	51	theme	Bullet	210:215	arg1	head					235:238	head	235:238	head	235:238	The present study deals with the isolation of sulfated polysaccharides (Ps) from the Bullet tuna by-products (head, skin and bones).
29801845	3	52	with	mixture	420:426	arg1	amounts					457:463	high amounts	452:463	high amounts of glucuronic and galacturonic acids	452:500	Tuna extracted-Ps contained a mixture of neutral sugars, with high amounts of glucuronic and galacturonic acids and presented different molecular weights.
29801845	3	52	with	mixture	420:426	arg1	acids					496:500	glucuronic and galacturonic acids	468:500	glucuronic and galacturonic acids	468:500	Tuna extracted-Ps contained a mixture of neutral sugars, with high amounts of glucuronic and galacturonic acids and presented different molecular weights.
29801845	3	53	theme	high	452:455	arg1	amounts					457:463	high amounts	452:463	high amounts of glucuronic and galacturonic acids	452:500	Tuna extracted-Ps contained a mixture of neutral sugars, with high amounts of glucuronic and galacturonic acids and presented different molecular weights.
29801845	3	53	theme	high	452:455	arg1	acids					496:500	glucuronic and galacturonic acids	468:500	glucuronic and galacturonic acids	468:500	Tuna extracted-Ps contained a mixture of neutral sugars, with high amounts of glucuronic and galacturonic acids and presented different molecular weights.
29801845	1	54	theme	tuna	217:220	arg1	by-products					222:232	the Bullet tuna by-products	206:232	the Bullet tuna by-products (head, skin and bones)	206:255	The present study deals with the isolation of sulfated polysaccharides (Ps) from the Bullet tuna by-products (head, skin and bones).
29801845	1	54	theme	tuna	217:220	arg1	bones					250:254	bones	250:254	bones	250:254	The present study deals with the isolation of sulfated polysaccharides (Ps) from the Bullet tuna by-products (head, skin and bones).
29801845	1	54	theme	tuna	217:220	arg1	skin					241:244	skin	241:244	skin	241:244	The present study deals with the isolation of sulfated polysaccharides (Ps) from the Bullet tuna by-products (head, skin and bones).
29801845	1	54	theme	tuna	217:220	arg1	head					235:238	head	235:238	head	235:238	The present study deals with the isolation of sulfated polysaccharides (Ps) from the Bullet tuna by-products (head, skin and bones).
29801845	0	55	theme	Bullet	85:90	arg1	tuna					92:95	Bullet tuna	85:95	Bullet tuna	85:95	Bioactive potential and structural characterization of sulfated polysaccharides from Bullet tuna (Auxis Rochei) by-products.
29801845	3	56	theme	neutral	431:437	arg1	sugars					439:444	neutral sugars	431:444	neutral sugars	431:444	Tuna extracted-Ps contained a mixture of neutral sugars, with high amounts of glucuronic and galacturonic acids and presented different molecular weights.
29801845	4	57	theme	FTIR	609:612	arg1	analysis					614:621	FTIR analysis	609:621	FTIR analysis	609:621	The presence of sulfate groups in different Ps was confirmed by FTIR analysis.
29801845	3	58	theme	galacturonic	483:494	arg1	acids					496:500	glucuronic and galacturonic acids	468:500	glucuronic and galacturonic acids	468:500	Tuna extracted-Ps contained a mixture of neutral sugars, with high amounts of glucuronic and galacturonic acids and presented different molecular weights.
29801845	4	59	theme	different	579:587	arg1	Ps					589:590	different Ps	579:590	different Ps	579:590	The presence of sulfate groups in different Ps was confirmed by FTIR analysis.
29801845	3	60	theme	acids	496:500	arg1	amounts					457:463	high amounts	452:463	high amounts of glucuronic and galacturonic acids	452:500	Tuna extracted-Ps contained a mixture of neutral sugars, with high amounts of glucuronic and galacturonic acids and presented different molecular weights.
29801845	3	60	theme	acids	496:500	arg1	acids					496:500	glucuronic and galacturonic acids	468:500	glucuronic and galacturonic acids	468:500	Tuna extracted-Ps contained a mixture of neutral sugars, with high amounts of glucuronic and galacturonic acids and presented different molecular weights.
31681352	4	0	theme	distinct	694:701	arg1	walls					703:707	the distinct walls	690:707	the distinct walls of grasses but also of those associated with xyloglucan and pectin synthesis	690:784	High levels of transcript abundances were not confined to genes associated with the distinct walls of grasses but also of those associated with xyloglucan and pectin synthesis.
31681352	1	1	from	species	168:174	arg1	composition					134:144	composition	134:144	composition from other angiosperm species	134:174	Grasses and related commelinid monocot species synthesize cell walls distinct in composition from other angiosperm species.
31681352	2	2	theme	protein	339:345	arg1	constituents					347:358	all cell-wall polysaccharide, phenylpropanoid, and protein constituents	288:358	constituents	347:358	With few exceptions, the genomes of all angiosperms contain the genes that encode the enzymes for synthesis of all cell-wall polysaccharide, phenylpropanoid, and protein constituents known in vascular plants.
31681352	5	3	from	synthesis	982:990	arg1	apparatus					1005:1013	the Golgi apparatus	995:1013	the Golgi apparatus	995:1013	Combined with proteomics data to confirm that expressed genes are translated, we propose that the distinctive cell-wall composition of grasses results from sorting downstream from their sites of synthesis in the Golgi apparatus and hydrolysis of the uncharacteristic polysaccharides and not from differential expression of synthases of grass-specific polysaccharides.
31681352	2	4	contain	contain	229:235	arg1	genomes					202:208	the genomes	198:208	the genomes of all angiosperms	198:227	With few exceptions, the genomes of all angiosperms contain the genes that encode the enzymes for synthesis of all cell-wall polysaccharide, phenylpropanoid, and protein constituents known in vascular plants.
31681352	2	4	contain	contain	229:235	arg2	genes					241:245	the genes	237:245	the genes that encode the enzymes for synthesis of all cell-wall polysaccharide, phenylpropanoid, and protein constituents known in vascular plants	237:383	With few exceptions, the genomes of all angiosperms contain the genes that encode the enzymes for synthesis of all cell-wall polysaccharide, phenylpropanoid, and protein constituents known in vascular plants.
31681352	2	4	contain	contain	229:235	arg2	enzymes					263:269	the enzymes	259:269	the enzymes for synthesis of all cell-wall polysaccharide, phenylpropanoid, and protein constituents known in vascular plants	259:383	With few exceptions, the genomes of all angiosperms contain the genes that encode the enzymes for synthesis of all cell-wall polysaccharide, phenylpropanoid, and protein constituents known in vascular plants.
31681352	5	5	theme	distinctive	885:895	arg1	composition					907:917	the distinctive cell-wall composition	881:917	the distinctive cell-wall composition of grasses	881:928	Combined with proteomics data to confirm that expressed genes are translated, we propose that the distinctive cell-wall composition of grasses results from sorting downstream from their sites of synthesis in the Golgi apparatus and hydrolysis of the uncharacteristic polysaccharides and not from differential expression of synthases of grass-specific polysaccharides.
31681352	1	6	theme	other	151:155	arg1	species					168:174	other angiosperm species	151:174	other angiosperm species	151:174	Grasses and related commelinid monocot species synthesize cell walls distinct in composition from other angiosperm species.
31681352	5	7	from	expression	1096:1105	arg1	downstream					951:960	downstream	951:960	downstream	951:960	Combined with proteomics data to confirm that expressed genes are translated, we propose that the distinctive cell-wall composition of grasses results from sorting downstream from their sites of synthesis in the Golgi apparatus and hydrolysis of the uncharacteristic polysaccharides and not from differential expression of synthases of grass-specific polysaccharides.
31681352	3	8	dep	stem	501:504	arg1	captured					506:513	captured	506:513	stem captured the expression of cell-wall-related genes associated with primary or secondary wall formation	501:607	RNA-seq analysis of transcripts expressed during development of the upper and lower internodes of maize (Zea mays) stem captured the expression of cell-wall-related genes associated with primary or secondary wall formation.
31681352	2	9	theme	cell-wall	292:300	arg1	phenylpropanoid					318:332	all cell-wall polysaccharide, phenylpropanoid, and protein constituents	288:358	phenylpropanoid	318:332	With few exceptions, the genomes of all angiosperms contain the genes that encode the enzymes for synthesis of all cell-wall polysaccharide, phenylpropanoid, and protein constituents known in vascular plants.
31681352	5	10	theme	synthases	1110:1118	arg1	expression					1096:1105	differential expression	1083:1105	differential expression of synthases of grass-specific polysaccharides	1083:1152	Combined with proteomics data to confirm that expressed genes are translated, we propose that the distinctive cell-wall composition of grasses results from sorting downstream from their sites of synthesis in the Golgi apparatus and hydrolysis of the uncharacteristic polysaccharides and not from differential expression of synthases of grass-specific polysaccharides.
31681352	3	11	theme	Zea	491:493	arg1	maize					484:488	maize	484:488	maize (Zea mays)	484:499	RNA-seq analysis of transcripts expressed during development of the upper and lower internodes of maize (Zea mays) stem captured the expression of cell-wall-related genes associated with primary or secondary wall formation.
31681352	3	11	theme	Zea	491:493	arg1	mays					495:498	Zea mays	491:498	Zea mays	491:498	RNA-seq analysis of transcripts expressed during development of the upper and lower internodes of maize (Zea mays) stem captured the expression of cell-wall-related genes associated with primary or secondary wall formation.
31681352	1	12	theme	distinct	122:129	arg1	walls					116:120	cell walls	111:120	cell walls distinct in composition from other angiosperm species	111:174	Grasses and related commelinid monocot species synthesize cell walls distinct in composition from other angiosperm species.
31681352	5	13	theme	expressed	833:841	arg1	genes					843:847	expressed genes	833:847	expressed genes	833:847	Combined with proteomics data to confirm that expressed genes are translated, we propose that the distinctive cell-wall composition of grasses results from sorting downstream from their sites of synthesis in the Golgi apparatus and hydrolysis of the uncharacteristic polysaccharides and not from differential expression of synthases of grass-specific polysaccharides.
31681352	1	14	from	composition	134:144	arg1	distinct					122:129	distinct	122:129	distinct	122:129	Grasses and related commelinid monocot species synthesize cell walls distinct in composition from other angiosperm species.
31681352	5	15	from	apparatus	1005:1013	arg1	sites					973:977	their sites	967:977	their sites of synthesis in the Golgi apparatus and hydrolysis of the uncharacteristic polysaccharides	967:1068	Combined with proteomics data to confirm that expressed genes are translated, we propose that the distinctive cell-wall composition of grasses results from sorting downstream from their sites of synthesis in the Golgi apparatus and hydrolysis of the uncharacteristic polysaccharides and not from differential expression of synthases of grass-specific polysaccharides.
31681352	5	15	from	apparatus	1005:1013	arg1	synthesis					982:990	synthesis	982:990	synthesis in the Golgi apparatus	982:1013	Combined with proteomics data to confirm that expressed genes are translated, we propose that the distinctive cell-wall composition of grasses results from sorting downstream from their sites of synthesis in the Golgi apparatus and hydrolysis of the uncharacteristic polysaccharides and not from differential expression of synthases of grass-specific polysaccharides.
31681352	5	15	from	apparatus	1005:1013	arg1	hydrolysis					1019:1028	hydrolysis	1019:1028	hydrolysis of the uncharacteristic polysaccharides	1019:1068	Combined with proteomics data to confirm that expressed genes are translated, we propose that the distinctive cell-wall composition of grasses results from sorting downstream from their sites of synthesis in the Golgi apparatus and hydrolysis of the uncharacteristic polysaccharides and not from differential expression of synthases of grass-specific polysaccharides.
31681352	1	16	theme	angiosperm	157:166	arg1	species					168:174	other angiosperm species	151:174	other angiosperm species	151:174	Grasses and related commelinid monocot species synthesize cell walls distinct in composition from other angiosperm species.
31681352	5	17	theme	cell-wall	897:905	arg1	composition					907:917	the distinctive cell-wall composition	881:917	the distinctive cell-wall composition of grasses	881:928	Combined with proteomics data to confirm that expressed genes are translated, we propose that the distinctive cell-wall composition of grasses results from sorting downstream from their sites of synthesis in the Golgi apparatus and hydrolysis of the uncharacteristic polysaccharides and not from differential expression of synthases of grass-specific polysaccharides.
31681352	5	18	theme	synthesis	982:990	arg1	sites					973:977	their sites	967:977	their sites of synthesis in the Golgi apparatus and hydrolysis of the uncharacteristic polysaccharides	967:1068	Combined with proteomics data to confirm that expressed genes are translated, we propose that the distinctive cell-wall composition of grasses results from sorting downstream from their sites of synthesis in the Golgi apparatus and hydrolysis of the uncharacteristic polysaccharides and not from differential expression of synthases of grass-specific polysaccharides.
31681352	5	18	theme	synthesis	982:990	arg1	synthesis					982:990	synthesis	982:990	synthesis in the Golgi apparatus	982:1013	Combined with proteomics data to confirm that expressed genes are translated, we propose that the distinctive cell-wall composition of grasses results from sorting downstream from their sites of synthesis in the Golgi apparatus and hydrolysis of the uncharacteristic polysaccharides and not from differential expression of synthases of grass-specific polysaccharides.
31681352	5	18	theme	synthesis	982:990	arg1	hydrolysis					1019:1028	hydrolysis	1019:1028	hydrolysis of the uncharacteristic polysaccharides	1019:1068	Combined with proteomics data to confirm that expressed genes are translated, we propose that the distinctive cell-wall composition of grasses results from sorting downstream from their sites of synthesis in the Golgi apparatus and hydrolysis of the uncharacteristic polysaccharides and not from differential expression of synthases of grass-specific polysaccharides.
31681352	5	19	theme	proteomics	801:810	arg1	data					812:815	proteomics data	801:815	proteomics data	801:815	Combined with proteomics data to confirm that expressed genes are translated, we propose that the distinctive cell-wall composition of grasses results from sorting downstream from their sites of synthesis in the Golgi apparatus and hydrolysis of the uncharacteristic polysaccharides and not from differential expression of synthases of grass-specific polysaccharides.
31681352	4	20	theme	abundances	636:645	arg1	levels					615:620	High levels	610:620	High levels of transcript abundances	610:645	High levels of transcript abundances were not confined to genes associated with the distinct walls of grasses but also of those associated with xyloglucan and pectin synthesis.
31681352	1	21	from	distinct	122:129	arg1	composition					134:144	composition	134:144	composition from other angiosperm species	134:174	Grasses and related commelinid monocot species synthesize cell walls distinct in composition from other angiosperm species.
31681352	1	22	theme	related	65:71	arg1	species					92:98	related commelinid monocot species	65:98	related commelinid monocot species	65:98	Grasses and related commelinid monocot species synthesize cell walls distinct in composition from other angiosperm species.
31681352	2	23	theme	vascular	369:376	arg1	plants					378:383	vascular plants	369:383	vascular plants	369:383	With few exceptions, the genomes of all angiosperms contain the genes that encode the enzymes for synthesis of all cell-wall polysaccharide, phenylpropanoid, and protein constituents known in vascular plants.
31681352	4	24	theme	grasses	712:718	arg1	walls					703:707	the distinct walls	690:707	the distinct walls of grasses but also of those associated with xyloglucan and pectin synthesis	690:784	High levels of transcript abundances were not confined to genes associated with the distinct walls of grasses but also of those associated with xyloglucan and pectin synthesis.
31681352	1	25	theme	commelinid	73:82	arg1	species					92:98	related commelinid monocot species	65:98	related commelinid monocot species	65:98	Grasses and related commelinid monocot species synthesize cell walls distinct in composition from other angiosperm species.
31681352	0	26	theme	Cell	17:20	arg1	Families					32:39	the Cell Wall Gene Families	13:39	the Cell Wall Gene Families of Grasses	13:50	Evolution of the Cell Wall Gene Families of Grasses.
31681352	1	27	theme	monocot	84:90	arg1	species					92:98	related commelinid monocot species	65:98	related commelinid monocot species	65:98	Grasses and related commelinid monocot species synthesize cell walls distinct in composition from other angiosperm species.
31681352	3	28	theme	secondary	584:592	arg1	formation					599:607	primary or secondary wall formation	573:607	primary or secondary wall formation	573:607	RNA-seq analysis of transcripts expressed during development of the upper and lower internodes of maize (Zea mays) stem captured the expression of cell-wall-related genes associated with primary or secondary wall formation.
31681352	4	29	theme	transcript	625:634	arg1	abundances					636:645	transcript abundances	625:645	transcript abundances	625:645	High levels of transcript abundances were not confined to genes associated with the distinct walls of grasses but also of those associated with xyloglucan and pectin synthesis.
31681352	4	30	dep	xyloglucan	754:763	arg1	synthesis					776:784	synthesis	776:784	synthesis	776:784	High levels of transcript abundances were not confined to genes associated with the distinct walls of grasses but also of those associated with xyloglucan and pectin synthesis.
31681352	3	31	theme	genes	551:555	arg1	expression					519:528	the expression	515:528	the expression of cell-wall-related genes associated with primary or secondary wall formation	515:607	RNA-seq analysis of transcripts expressed during development of the upper and lower internodes of maize (Zea mays) stem captured the expression of cell-wall-related genes associated with primary or secondary wall formation.
31681352	3	32	theme	lower	464:468	arg1	internodes					470:479	the upper and lower internodes	450:479	the upper and lower internodes of maize (Zea mays)	450:499	RNA-seq analysis of transcripts expressed during development of the upper and lower internodes of maize (Zea mays) stem captured the expression of cell-wall-related genes associated with primary or secondary wall formation.
31681352	0	33	theme	Gene	27:30	arg1	Families					32:39	the Cell Wall Gene Families	13:39	the Cell Wall Gene Families of Grasses	13:50	Evolution of the Cell Wall Gene Families of Grasses.
31681352	2	34	theme	phenylpropanoid	318:332	arg1	synthesis					275:283	synthesis	275:283	synthesis of all cell-wall polysaccharide, phenylpropanoid, and protein constituents known in vascular plants	275:383	With few exceptions, the genomes of all angiosperms contain the genes that encode the enzymes for synthesis of all cell-wall polysaccharide, phenylpropanoid, and protein constituents known in vascular plants.
31681352	5	35	from	sites	973:977	arg1	downstream					951:960	downstream	951:960	downstream	951:960	Combined with proteomics data to confirm that expressed genes are translated, we propose that the distinctive cell-wall composition of grasses results from sorting downstream from their sites of synthesis in the Golgi apparatus and hydrolysis of the uncharacteristic polysaccharides and not from differential expression of synthases of grass-specific polysaccharides.
31681352	5	35	from	sites	973:977	arg1	apparatus					1005:1013	the Golgi apparatus	995:1013	the Golgi apparatus	995:1013	Combined with proteomics data to confirm that expressed genes are translated, we propose that the distinctive cell-wall composition of grasses results from sorting downstream from their sites of synthesis in the Golgi apparatus and hydrolysis of the uncharacteristic polysaccharides and not from differential expression of synthases of grass-specific polysaccharides.
31681352	5	36	theme	differential	1083:1094	arg1	expression					1096:1105	differential expression	1083:1105	differential expression of synthases of grass-specific polysaccharides	1083:1152	Combined with proteomics data to confirm that expressed genes are translated, we propose that the distinctive cell-wall composition of grasses results from sorting downstream from their sites of synthesis in the Golgi apparatus and hydrolysis of the uncharacteristic polysaccharides and not from differential expression of synthases of grass-specific polysaccharides.
31681352	2	37	theme	angiosperms	217:227	arg1	genomes					202:208	the genomes	198:208	the genomes of all angiosperms	198:227	With few exceptions, the genomes of all angiosperms contain the genes that encode the enzymes for synthesis of all cell-wall polysaccharide, phenylpropanoid, and protein constituents known in vascular plants.
31681352	5	38	from	hydrolysis	1019:1028	arg1	apparatus					1005:1013	the Golgi apparatus	995:1013	the Golgi apparatus	995:1013	Combined with proteomics data to confirm that expressed genes are translated, we propose that the distinctive cell-wall composition of grasses results from sorting downstream from their sites of synthesis in the Golgi apparatus and hydrolysis of the uncharacteristic polysaccharides and not from differential expression of synthases of grass-specific polysaccharides.
31681352	0	39	theme	Wall	22:25	arg1	Families					32:39	the Cell Wall Gene Families	13:39	the Cell Wall Gene Families of Grasses	13:50	Evolution of the Cell Wall Gene Families of Grasses.
31681352	3	40	theme	transcripts	406:416	arg1	analysis					394:401	RNA-seq analysis	386:401	RNA-seq analysis of transcripts expressed during development of the upper and lower internodes of maize (Zea mays)	386:499	RNA-seq analysis of transcripts expressed during development of the upper and lower internodes of maize (Zea mays) stem captured the expression of cell-wall-related genes associated with primary or secondary wall formation.
31681352	3	41	theme	wall	594:597	arg1	formation					599:607	primary or secondary wall formation	573:607	primary or secondary wall formation	573:607	RNA-seq analysis of transcripts expressed during development of the upper and lower internodes of maize (Zea mays) stem captured the expression of cell-wall-related genes associated with primary or secondary wall formation.
31681352	3	42	theme	upper	454:458	arg1	internodes					470:479	the upper and lower internodes	450:479	the upper and lower internodes of maize (Zea mays)	450:499	RNA-seq analysis of transcripts expressed during development of the upper and lower internodes of maize (Zea mays) stem captured the expression of cell-wall-related genes associated with primary or secondary wall formation.
31681352	3	43	theme	RNA-seq	386:392	arg1	analysis					394:401	RNA-seq analysis	386:401	RNA-seq analysis of transcripts expressed during development of the upper and lower internodes of maize (Zea mays)	386:499	RNA-seq analysis of transcripts expressed during development of the upper and lower internodes of maize (Zea mays) stem captured the expression of cell-wall-related genes associated with primary or secondary wall formation.
31681352	3	44	theme	primary	573:579	arg1	formation					599:607	primary or secondary wall formation	573:607	primary or secondary wall formation	573:607	RNA-seq analysis of transcripts expressed during development of the upper and lower internodes of maize (Zea mays) stem captured the expression of cell-wall-related genes associated with primary or secondary wall formation.
31681352	5	45	theme	grass-specific	1123:1136	arg1	polysaccharides					1138:1152	grass-specific polysaccharides	1123:1152	grass-specific polysaccharides	1123:1152	Combined with proteomics data to confirm that expressed genes are translated, we propose that the distinctive cell-wall composition of grasses results from sorting downstream from their sites of synthesis in the Golgi apparatus and hydrolysis of the uncharacteristic polysaccharides and not from differential expression of synthases of grass-specific polysaccharides.
31681352	3	46	theme	maize	484:488	arg1	internodes					470:479	the upper and lower internodes	450:479	the upper and lower internodes of maize (Zea mays)	450:499	RNA-seq analysis of transcripts expressed during development of the upper and lower internodes of maize (Zea mays) stem captured the expression of cell-wall-related genes associated with primary or secondary wall formation.
31681352	0	47	theme	Families	32:39	arg1	Evolution					0:8	Evolution	0:8	Evolution of the Cell Wall Gene Families of Grasses.	0:51	Evolution of the Cell Wall Gene Families of Grasses.
31681352	5	48	theme	polysaccharides	1138:1152	arg1	synthases					1110:1118	synthases	1110:1118	synthases of grass-specific polysaccharides	1110:1152	Combined with proteomics data to confirm that expressed genes are translated, we propose that the distinctive cell-wall composition of grasses results from sorting downstream from their sites of synthesis in the Golgi apparatus and hydrolysis of the uncharacteristic polysaccharides and not from differential expression of synthases of grass-specific polysaccharides.
31681352	5	49	theme	uncharacteristic	1037:1052	arg1	polysaccharides					1054:1068	the uncharacteristic polysaccharides	1033:1068	the uncharacteristic polysaccharides	1033:1068	Combined with proteomics data to confirm that expressed genes are translated, we propose that the distinctive cell-wall composition of grasses results from sorting downstream from their sites of synthesis in the Golgi apparatus and hydrolysis of the uncharacteristic polysaccharides and not from differential expression of synthases of grass-specific polysaccharides.
31681352	5	50	theme	downstream	951:960	arg1	sorting					943:949	sorting	943:949	sorting downstream from their sites of synthesis in the Golgi apparatus and hydrolysis of the uncharacteristic polysaccharides and not from differential expression of synthases of grass-specific polysaccharides	943:1152	Combined with proteomics data to confirm that expressed genes are translated, we propose that the distinctive cell-wall composition of grasses results from sorting downstream from their sites of synthesis in the Golgi apparatus and hydrolysis of the uncharacteristic polysaccharides and not from differential expression of synthases of grass-specific polysaccharides.
31681352	1	51	theme	cell	111:114	arg1	walls					116:120	cell walls	111:120	cell walls distinct in composition from other angiosperm species	111:174	Grasses and related commelinid monocot species synthesize cell walls distinct in composition from other angiosperm species.
31681352	5	52	theme	polysaccharides	1054:1068	arg1	hydrolysis					1019:1028	hydrolysis	1019:1028	hydrolysis of the uncharacteristic polysaccharides	1019:1068	Combined with proteomics data to confirm that expressed genes are translated, we propose that the distinctive cell-wall composition of grasses results from sorting downstream from their sites of synthesis in the Golgi apparatus and hydrolysis of the uncharacteristic polysaccharides and not from differential expression of synthases of grass-specific polysaccharides.
31681352	5	52	theme	polysaccharides	1054:1068	arg1	synthesis					982:990	synthesis	982:990	synthesis in the Golgi apparatus	982:1013	Combined with proteomics data to confirm that expressed genes are translated, we propose that the distinctive cell-wall composition of grasses results from sorting downstream from their sites of synthesis in the Golgi apparatus and hydrolysis of the uncharacteristic polysaccharides and not from differential expression of synthases of grass-specific polysaccharides.
31681352	5	53	theme	hydrolysis	1019:1028	arg1	sites					973:977	their sites	967:977	their sites of synthesis in the Golgi apparatus and hydrolysis of the uncharacteristic polysaccharides	967:1068	Combined with proteomics data to confirm that expressed genes are translated, we propose that the distinctive cell-wall composition of grasses results from sorting downstream from their sites of synthesis in the Golgi apparatus and hydrolysis of the uncharacteristic polysaccharides and not from differential expression of synthases of grass-specific polysaccharides.
31681352	5	53	theme	hydrolysis	1019:1028	arg1	synthesis					982:990	synthesis	982:990	synthesis in the Golgi apparatus	982:1013	Combined with proteomics data to confirm that expressed genes are translated, we propose that the distinctive cell-wall composition of grasses results from sorting downstream from their sites of synthesis in the Golgi apparatus and hydrolysis of the uncharacteristic polysaccharides and not from differential expression of synthases of grass-specific polysaccharides.
31681352	5	53	theme	hydrolysis	1019:1028	arg1	hydrolysis					1019:1028	hydrolysis	1019:1028	hydrolysis of the uncharacteristic polysaccharides	1019:1068	Combined with proteomics data to confirm that expressed genes are translated, we propose that the distinctive cell-wall composition of grasses results from sorting downstream from their sites of synthesis in the Golgi apparatus and hydrolysis of the uncharacteristic polysaccharides and not from differential expression of synthases of grass-specific polysaccharides.
31681352	3	54	theme	internodes	470:479	arg1	development					435:445	development	435:445	development of the upper and lower internodes of maize (Zea mays)	435:499	RNA-seq analysis of transcripts expressed during development of the upper and lower internodes of maize (Zea mays) stem captured the expression of cell-wall-related genes associated with primary or secondary wall formation.
31681352	3	55	theme	cell-wall-related	533:549	arg1	genes					551:555	cell-wall-related genes	533:555	cell-wall-related genes associated with primary or secondary wall formation	533:607	RNA-seq analysis of transcripts expressed during development of the upper and lower internodes of maize (Zea mays) stem captured the expression of cell-wall-related genes associated with primary or secondary wall formation.
31681352	4	56	theme	those	732:736	arg1	walls					703:707	the distinct walls	690:707	the distinct walls of grasses but also of those associated with xyloglucan and pectin synthesis	690:784	High levels of transcript abundances were not confined to genes associated with the distinct walls of grasses but also of those associated with xyloglucan and pectin synthesis.
31681352	2	57	theme	polysaccharide	302:315	arg1	phenylpropanoid					318:332	all cell-wall polysaccharide, phenylpropanoid, and protein constituents	288:358	phenylpropanoid	318:332	With few exceptions, the genomes of all angiosperms contain the genes that encode the enzymes for synthesis of all cell-wall polysaccharide, phenylpropanoid, and protein constituents known in vascular plants.
31681352	5	58	theme	grasses	922:928	arg1	composition					907:917	the distinctive cell-wall composition	881:917	the distinctive cell-wall composition of grasses	881:928	Combined with proteomics data to confirm that expressed genes are translated, we propose that the distinctive cell-wall composition of grasses results from sorting downstream from their sites of synthesis in the Golgi apparatus and hydrolysis of the uncharacteristic polysaccharides and not from differential expression of synthases of grass-specific polysaccharides.
31681352	2	59	theme	few	182:184	arg1	exceptions					186:195	few exceptions	182:195	few exceptions	182:195	With few exceptions, the genomes of all angiosperms contain the genes that encode the enzymes for synthesis of all cell-wall polysaccharide, phenylpropanoid, and protein constituents known in vascular plants.
31681352	5	60	theme	Golgi	999:1003	arg1	apparatus					1005:1013	the Golgi apparatus	995:1013	the Golgi apparatus	995:1013	Combined with proteomics data to confirm that expressed genes are translated, we propose that the distinctive cell-wall composition of grasses results from sorting downstream from their sites of synthesis in the Golgi apparatus and hydrolysis of the uncharacteristic polysaccharides and not from differential expression of synthases of grass-specific polysaccharides.
31681352	0	61	theme	Grasses	44:50	arg1	Families					32:39	the Cell Wall Gene Families	13:39	the Cell Wall Gene Families of Grasses	13:50	Evolution of the Cell Wall Gene Families of Grasses.
31681352	2	62	theme	constituents	347:358	arg1	synthesis					275:283	synthesis	275:283	synthesis of all cell-wall polysaccharide, phenylpropanoid, and protein constituents known in vascular plants	275:383	With few exceptions, the genomes of all angiosperms contain the genes that encode the enzymes for synthesis of all cell-wall polysaccharide, phenylpropanoid, and protein constituents known in vascular plants.
31681352	4	63	theme	High	610:613	arg1	levels					615:620	High levels	610:620	High levels of transcript abundances	610:645	High levels of transcript abundances were not confined to genes associated with the distinct walls of grasses but also of those associated with xyloglucan and pectin synthesis.
31489348	1	0	theme	stems	266:270	arg1	morphology					191:200	morphology	191:200	morphology	191:200	This article presents experimental data describing the morphology and the cell wall monosaccharide content of rosettes and flower stems of five Arabidopsis thaliana ecotypes grown at two contrasted temperatures.
31489348	1	0	theme	stems	266:270	arg1	content					235:241	the cell wall monosaccharide content	206:241	the cell wall monosaccharide content	206:241	This article presents experimental data describing the morphology and the cell wall monosaccharide content of rosettes and flower stems of five Arabidopsis thaliana ecotypes grown at two contrasted temperatures.
31489348	0	1	theme	ecotypes	81:88	arg1	Phenotyping					0:10	Phenotyping	0:10	Phenotyping	0:10	Phenotyping and cell wall polysaccharide composition dataset of five arabidopsis ecotypes grown at optimal or sub-optimal temperatures.
31489348	0	1	theme	ecotypes	81:88	arg1	dataset					53:59	cell wall polysaccharide composition dataset	16:59	cell wall polysaccharide composition dataset	16:59	Phenotyping and cell wall polysaccharide composition dataset of five arabidopsis ecotypes grown at optimal or sub-optimal temperatures.
31489348	3	2	dep	temperatures	600:611	arg1	22 °C					624:628	22 °C	624:628	22 °C	624:628	The well-described and sequenced Columbia (Col) ecotype and four newly-described Pyrenees ecotypes (Duruflé et al., 2019) have been grown at two different temperatures (15 °C and 22 °C).
31489348	3	2	dep	temperatures	600:611	arg1	15 °C					614:618	15 °C	614:618	15 °C	614:618	The well-described and sequenced Columbia (Col) ecotype and four newly-described Pyrenees ecotypes (Duruflé et al., 2019) have been grown at two different temperatures (15 °C and 22 °C).
31489348	3	2	dep	temperatures	600:611	arg1	temperatures					600:611	two different temperatures	586:611	two different temperatures (15 °C and 22 °C)	586:629	The well-described and sequenced Columbia (Col) ecotype and four newly-described Pyrenees ecotypes (Duruflé et al., 2019) have been grown at two different temperatures (15 °C and 22 °C).
31489348	0	3	theme	optimal	99:105	arg1	temperatures					122:133	optimal or sub-optimal temperatures	99:133	optimal or sub-optimal temperatures	99:133	Phenotyping and cell wall polysaccharide composition dataset of five arabidopsis ecotypes grown at optimal or sub-optimal temperatures.
31489348	4	4	from	stage	833:837	arg1	mass					881:884	mass	881:884	mass	881:884	For macrophenotyping, we provide dataset regarding (i) rosettes such as measurement of diameter and fresh mass as well as number of leaves just before bolting and (ii) floral stems at the first flower stage such as length, number of cauline leaves, mass and diameter at its base.
31489348	4	4	from	stage	833:837	arg1	number					855:860	number	855:860	number of cauline leaves	855:878	For macrophenotyping, we provide dataset regarding (i) rosettes such as measurement of diameter and fresh mass as well as number of leaves just before bolting and (ii) floral stems at the first flower stage such as length, number of cauline leaves, mass and diameter at its base.
31489348	4	4	from	stage	833:837	arg1	diameter					890:897	diameter	890:897	diameter	890:897	For macrophenotyping, we provide dataset regarding (i) rosettes such as measurement of diameter and fresh mass as well as number of leaves just before bolting and (ii) floral stems at the first flower stage such as length, number of cauline leaves, mass and diameter at its base.
31489348	4	4	from	stage	833:837	arg1	stems					807:811	floral stems	800:811	(ii) floral stems at the first flower stage such as length, number of cauline leaves, mass and diameter	795:897	For macrophenotyping, we provide dataset regarding (i) rosettes such as measurement of diameter and fresh mass as well as number of leaves just before bolting and (ii) floral stems at the first flower stage such as length, number of cauline leaves, mass and diameter at its base.
31489348	4	4	from	stage	833:837	arg1	bolting					783:789	bolting	783:789	bolting	783:789	For macrophenotyping, we provide dataset regarding (i) rosettes such as measurement of diameter and fresh mass as well as number of leaves just before bolting and (ii) floral stems at the first flower stage such as length, number of cauline leaves, mass and diameter at its base.
31489348	4	4	from	stage	833:837	arg1	length					847:852	length	847:852	length	847:852	For macrophenotyping, we provide dataset regarding (i) rosettes such as measurement of diameter and fresh mass as well as number of leaves just before bolting and (ii) floral stems at the first flower stage such as length, number of cauline leaves, mass and diameter at its base.
31489348	6	5	theme	different	1127:1135	arg1	ecotypes					1137:1144	the different ecotypes	1123:1144	the different ecotypes	1123:1144	All these data are markers to differentiate both growth temperatures and the different ecotypes.
31489348	7	6	theme	A.	1228:1229	arg1	ecotypes					1240:1247	A. thaliana ecotypes	1228:1247	A. thaliana ecotypes	1228:1247	They constitute a valuable resource for the community to study the adaptation of A. thaliana ecotypes to sub-optimal temperature growth conditions.
31489348	4	7	theme	fresh	732:736	arg1	mass					738:741	fresh mass	732:741	fresh mass	732:741	For macrophenotyping, we provide dataset regarding (i) rosettes such as measurement of diameter and fresh mass as well as number of leaves just before bolting and (ii) floral stems at the first flower stage such as length, number of cauline leaves, mass and diameter at its base.
31489348	1	8	theme	Arabidopsis	280:290	arg1	ecotypes					301:308	five Arabidopsis thaliana ecotypes	275:308	five Arabidopsis thaliana ecotypes grown at two contrasted temperatures	275:345	This article presents experimental data describing the morphology and the cell wall monosaccharide content of rosettes and flower stems of five Arabidopsis thaliana ecotypes grown at two contrasted temperatures.
31489348	0	9	theme	sub-optimal	110:120	arg1	temperatures					122:133	optimal or sub-optimal temperatures	99:133	optimal or sub-optimal temperatures	99:133	Phenotyping and cell wall polysaccharide composition dataset of five arabidopsis ecotypes grown at optimal or sub-optimal temperatures.
31489348	4	10	theme	diameter	719:726	arg1	measurement					704:714	measurement	704:714	measurement of diameter and fresh mass as well as number of leaves just before bolting and (ii) floral stems at the first flower stage such as length, number of cauline leaves, mass and diameter at its base	704:909	For macrophenotyping, we provide dataset regarding (i) rosettes such as measurement of diameter and fresh mass as well as number of leaves just before bolting and (ii) floral stems at the first flower stage such as length, number of cauline leaves, mass and diameter at its base.
31489348	4	10	theme	diameter	719:726	arg1	number					754:759	number	754:759	measurement of diameter and fresh mass as well as number of leaves just before bolting and (ii) floral stems at the first flower stage such as length, number of cauline leaves, mass and diameter at its base	704:909	For macrophenotyping, we provide dataset regarding (i) rosettes such as measurement of diameter and fresh mass as well as number of leaves just before bolting and (ii) floral stems at the first flower stage such as length, number of cauline leaves, mass and diameter at its base.
31489348	5	11	theme	cell	922:925	arg1	composition					932:942	cell wall composition	922:942	cell wall composition	922:942	Regarding cell wall composition, we provide data of quantification of seven monosaccharides and the reconstruction three polysaccharides.
31489348	2	12	theme	quantification	429:442	arg1	data					406:409	data	406:409	data of monosaccharide quantification	406:442	Besides, cell wall polysaccharides are reconstructed from data of monosaccharide quantification.
31489348	3	13	theme	well-described	449:462	arg1	ecotype					493:499	The well-described and sequenced Columbia (Col) ecotype	445:499	The well-described and sequenced Columbia (Col) ecotype	445:499	The well-described and sequenced Columbia (Col) ecotype and four newly-described Pyrenees ecotypes (Duruflé et al., 2019) have been grown at two different temperatures (15 °C and 22 °C).
31489348	7	14	theme	valuable	1165:1172	arg1	resource					1174:1181	a valuable resource	1163:1181	a valuable resource for the community to study the adaptation of A. thaliana ecotypes to sub-optimal temperature growth conditions	1163:1292	They constitute a valuable resource for the community to study the adaptation of A. thaliana ecotypes to sub-optimal temperature growth conditions.
31489348	4	15	dep	rosettes	687:694	arg1	i					684:684	i	684:684	i	684:684	For macrophenotyping, we provide dataset regarding (i) rosettes such as measurement of diameter and fresh mass as well as number of leaves just before bolting and (ii) floral stems at the first flower stage such as length, number of cauline leaves, mass and diameter at its base.
31489348	2	16	theme	monosaccharide	414:427	arg1	quantification					429:442	monosaccharide quantification	414:442	monosaccharide quantification	414:442	Besides, cell wall polysaccharides are reconstructed from data of monosaccharide quantification.
31489348	4	17	theme	flower	826:831	arg1	stage					833:837	the first flower stage	816:837	the first flower stage	816:837	For macrophenotyping, we provide dataset regarding (i) rosettes such as measurement of diameter and fresh mass as well as number of leaves just before bolting and (ii) floral stems at the first flower stage such as length, number of cauline leaves, mass and diameter at its base.
31489348	4	18	dep	cauline	865:871	arg1	leaves					873:878	leaves	873:878	leaves	873:878	For macrophenotyping, we provide dataset regarding (i) rosettes such as measurement of diameter and fresh mass as well as number of leaves just before bolting and (ii) floral stems at the first flower stage such as length, number of cauline leaves, mass and diameter at its base.
31489348	3	19	theme	different	590:598	arg1	22 °C					624:628	22 °C	624:628	22 °C	624:628	The well-described and sequenced Columbia (Col) ecotype and four newly-described Pyrenees ecotypes (Duruflé et al., 2019) have been grown at two different temperatures (15 °C and 22 °C).
31489348	3	19	theme	different	590:598	arg1	15 °C					614:618	15 °C	614:618	15 °C	614:618	The well-described and sequenced Columbia (Col) ecotype and four newly-described Pyrenees ecotypes (Duruflé et al., 2019) have been grown at two different temperatures (15 °C and 22 °C).
31489348	3	19	theme	different	590:598	arg1	temperatures					600:611	two different temperatures	586:611	two different temperatures (15 °C and 22 °C)	586:629	The well-described and sequenced Columbia (Col) ecotype and four newly-described Pyrenees ecotypes (Duruflé et al., 2019) have been grown at two different temperatures (15 °C and 22 °C).
31489348	7	20	theme	thaliana	1231:1238	arg1	ecotypes					1240:1247	A. thaliana ecotypes	1228:1247	A. thaliana ecotypes	1228:1247	They constitute a valuable resource for the community to study the adaptation of A. thaliana ecotypes to sub-optimal temperature growth conditions.
31489348	3	21	theme	sequenced	468:476	arg1	ecotype					493:499	The well-described and sequenced Columbia (Col) ecotype	445:499	The well-described and sequenced Columbia (Col) ecotype	445:499	The well-described and sequenced Columbia (Col) ecotype and four newly-described Pyrenees ecotypes (Duruflé et al., 2019) have been grown at two different temperatures (15 °C and 22 °C).
31489348	1	22	theme	thaliana	292:299	arg1	ecotypes					301:308	five Arabidopsis thaliana ecotypes	275:308	five Arabidopsis thaliana ecotypes grown at two contrasted temperatures	275:345	This article presents experimental data describing the morphology and the cell wall monosaccharide content of rosettes and flower stems of five Arabidopsis thaliana ecotypes grown at two contrasted temperatures.
31489348	4	23	theme	first	820:824	arg1	stage					833:837	the first flower stage	816:837	the first flower stage	816:837	For macrophenotyping, we provide dataset regarding (i) rosettes such as measurement of diameter and fresh mass as well as number of leaves just before bolting and (ii) floral stems at the first flower stage such as length, number of cauline leaves, mass and diameter at its base.
31489348	4	24	theme	mass	738:741	arg1	measurement					704:714	measurement	704:714	measurement of diameter and fresh mass as well as number of leaves just before bolting and (ii) floral stems at the first flower stage such as length, number of cauline leaves, mass and diameter at its base	704:909	For macrophenotyping, we provide dataset regarding (i) rosettes such as measurement of diameter and fresh mass as well as number of leaves just before bolting and (ii) floral stems at the first flower stage such as length, number of cauline leaves, mass and diameter at its base.
31489348	4	24	theme	mass	738:741	arg1	number					754:759	number	754:759	measurement of diameter and fresh mass as well as number of leaves just before bolting and (ii) floral stems at the first flower stage such as length, number of cauline leaves, mass and diameter at its base	704:909	For macrophenotyping, we provide dataset regarding (i) rosettes such as measurement of diameter and fresh mass as well as number of leaves just before bolting and (ii) floral stems at the first flower stage such as length, number of cauline leaves, mass and diameter at its base.
31489348	3	25	theme	Columbia	478:485	arg1	ecotype					493:499	The well-described and sequenced Columbia (Col) ecotype	445:499	The well-described and sequenced Columbia (Col) ecotype	445:499	The well-described and sequenced Columbia (Col) ecotype and four newly-described Pyrenees ecotypes (Duruflé et al., 2019) have been grown at two different temperatures (15 °C and 22 °C).
31489348	0	26	theme	wall	21:24	arg1	dataset					53:59	cell wall polysaccharide composition dataset	16:59	cell wall polysaccharide composition dataset	16:59	Phenotyping and cell wall polysaccharide composition dataset of five arabidopsis ecotypes grown at optimal or sub-optimal temperatures.
31489348	1	27	theme	ecotypes	301:308	arg1	rosettes					246:253	rosettes	246:253	rosettes	246:253	This article presents experimental data describing the morphology and the cell wall monosaccharide content of rosettes and flower stems of five Arabidopsis thaliana ecotypes grown at two contrasted temperatures.
31489348	1	27	theme	ecotypes	301:308	arg1	stems					266:270	flower stems	259:270	flower stems	259:270	This article presents experimental data describing the morphology and the cell wall monosaccharide content of rosettes and flower stems of five Arabidopsis thaliana ecotypes grown at two contrasted temperatures.
31489348	4	28	theme	floral	800:805	arg1	diameter					890:897	diameter	890:897	diameter	890:897	For macrophenotyping, we provide dataset regarding (i) rosettes such as measurement of diameter and fresh mass as well as number of leaves just before bolting and (ii) floral stems at the first flower stage such as length, number of cauline leaves, mass and diameter at its base.
31489348	4	28	theme	floral	800:805	arg1	length					847:852	length	847:852	length	847:852	For macrophenotyping, we provide dataset regarding (i) rosettes such as measurement of diameter and fresh mass as well as number of leaves just before bolting and (ii) floral stems at the first flower stage such as length, number of cauline leaves, mass and diameter at its base.
31489348	4	28	theme	floral	800:805	arg1	mass					881:884	mass	881:884	mass	881:884	For macrophenotyping, we provide dataset regarding (i) rosettes such as measurement of diameter and fresh mass as well as number of leaves just before bolting and (ii) floral stems at the first flower stage such as length, number of cauline leaves, mass and diameter at its base.
31489348	4	28	theme	floral	800:805	arg1	number					855:860	number	855:860	number of cauline leaves	855:878	For macrophenotyping, we provide dataset regarding (i) rosettes such as measurement of diameter and fresh mass as well as number of leaves just before bolting and (ii) floral stems at the first flower stage such as length, number of cauline leaves, mass and diameter at its base.
31489348	4	28	theme	floral	800:805	arg1	stems					807:811	floral stems	800:811	(ii) floral stems at the first flower stage such as length, number of cauline leaves, mass and diameter	795:897	For macrophenotyping, we provide dataset regarding (i) rosettes such as measurement of diameter and fresh mass as well as number of leaves just before bolting and (ii) floral stems at the first flower stage such as length, number of cauline leaves, mass and diameter at its base.
31489348	1	29	theme	cell	210:213	arg1	content					235:241	the cell wall monosaccharide content	206:241	the cell wall monosaccharide content	206:241	This article presents experimental data describing the morphology and the cell wall monosaccharide content of rosettes and flower stems of five Arabidopsis thaliana ecotypes grown at two contrasted temperatures.
31489348	0	30	theme	cell	16:19	arg1	dataset					53:59	cell wall polysaccharide composition dataset	16:59	cell wall polysaccharide composition dataset	16:59	Phenotyping and cell wall polysaccharide composition dataset of five arabidopsis ecotypes grown at optimal or sub-optimal temperatures.
31489348	5	31	theme	reconstruction	1012:1025	arg1	polysaccharides					1033:1047	the reconstruction three polysaccharides	1008:1047	the reconstruction three polysaccharides	1008:1047	Regarding cell wall composition, we provide data of quantification of seven monosaccharides and the reconstruction three polysaccharides.
31489348	6	32	theme	growth	1099:1104	arg1	temperatures					1106:1117	growth temperatures	1099:1117	growth temperatures	1099:1117	All these data are markers to differentiate both growth temperatures and the different ecotypes.
31489348	1	33	theme	wall	215:218	arg1	content					235:241	the cell wall monosaccharide content	206:241	the cell wall monosaccharide content	206:241	This article presents experimental data describing the morphology and the cell wall monosaccharide content of rosettes and flower stems of five Arabidopsis thaliana ecotypes grown at two contrasted temperatures.
31489348	0	34	theme	composition	41:51	arg1	dataset					53:59	cell wall polysaccharide composition dataset	16:59	cell wall polysaccharide composition dataset	16:59	Phenotyping and cell wall polysaccharide composition dataset of five arabidopsis ecotypes grown at optimal or sub-optimal temperatures.
31489348	7	35	theme	sub-optimal	1252:1262	arg1	conditions					1283:1292	sub-optimal temperature growth conditions	1252:1292	sub-optimal temperature growth conditions	1252:1292	They constitute a valuable resource for the community to study the adaptation of A. thaliana ecotypes to sub-optimal temperature growth conditions.
31489348	1	36	theme	monosaccharide	220:233	arg1	content					235:241	the cell wall monosaccharide content	206:241	the cell wall monosaccharide content	206:241	This article presents experimental data describing the morphology and the cell wall monosaccharide content of rosettes and flower stems of five Arabidopsis thaliana ecotypes grown at two contrasted temperatures.
31489348	0	37	theme	polysaccharide	26:39	arg1	dataset					53:59	cell wall polysaccharide composition dataset	16:59	cell wall polysaccharide composition dataset	16:59	Phenotyping and cell wall polysaccharide composition dataset of five arabidopsis ecotypes grown at optimal or sub-optimal temperatures.
31489348	5	38	theme	polysaccharides	1033:1047	arg1	quantification					964:977	quantification	964:977	quantification of seven monosaccharides and the reconstruction three polysaccharides	964:1047	Regarding cell wall composition, we provide data of quantification of seven monosaccharides and the reconstruction three polysaccharides.
31489348	7	39	theme	temperature	1264:1274	arg1	conditions					1283:1292	sub-optimal temperature growth conditions	1252:1292	sub-optimal temperature growth conditions	1252:1292	They constitute a valuable resource for the community to study the adaptation of A. thaliana ecotypes to sub-optimal temperature growth conditions.
31489348	1	40	theme	contrasted	323:332	arg1	temperatures					334:345	two contrasted temperatures	319:345	two contrasted temperatures	319:345	This article presents experimental data describing the morphology and the cell wall monosaccharide content of rosettes and flower stems of five Arabidopsis thaliana ecotypes grown at two contrasted temperatures.
31489348	5	41	theme	quantification	964:977	arg1	data					956:959	data	956:959	data of quantification of seven monosaccharides and the reconstruction three polysaccharides	956:1047	Regarding cell wall composition, we provide data of quantification of seven monosaccharides and the reconstruction three polysaccharides.
31489348	2	42	theme	wall	362:365	arg1	polysaccharides					367:381	cell wall polysaccharides	357:381	cell wall polysaccharides	357:381	Besides, cell wall polysaccharides are reconstructed from data of monosaccharide quantification.
31489348	1	43	dep	morphology	191:200	arg1	the					187:189	the	187:189	the	187:189	This article presents experimental data describing the morphology and the cell wall monosaccharide content of rosettes and flower stems of five Arabidopsis thaliana ecotypes grown at two contrasted temperatures.
31489348	7	44	theme	ecotypes	1240:1247	arg1	adaptation					1214:1223	the adaptation	1210:1223	the adaptation of A. thaliana ecotypes to sub-optimal temperature growth conditions	1210:1292	They constitute a valuable resource for the community to study the adaptation of A. thaliana ecotypes to sub-optimal temperature growth conditions.
31489348	2	45	theme	cell	357:360	arg1	polysaccharides					367:381	cell wall polysaccharides	357:381	cell wall polysaccharides	357:381	Besides, cell wall polysaccharides are reconstructed from data of monosaccharide quantification.
31489348	4	46	theme	cauline	865:871	arg1	diameter					890:897	diameter	890:897	diameter	890:897	For macrophenotyping, we provide dataset regarding (i) rosettes such as measurement of diameter and fresh mass as well as number of leaves just before bolting and (ii) floral stems at the first flower stage such as length, number of cauline leaves, mass and diameter at its base.
31489348	4	46	theme	cauline	865:871	arg1	mass					881:884	mass	881:884	mass	881:884	For macrophenotyping, we provide dataset regarding (i) rosettes such as measurement of diameter and fresh mass as well as number of leaves just before bolting and (ii) floral stems at the first flower stage such as length, number of cauline leaves, mass and diameter at its base.
31489348	4	46	theme	cauline	865:871	arg1	number					855:860	number	855:860	number of cauline leaves	855:878	For macrophenotyping, we provide dataset regarding (i) rosettes such as measurement of diameter and fresh mass as well as number of leaves just before bolting and (ii) floral stems at the first flower stage such as length, number of cauline leaves, mass and diameter at its base.
31489348	4	46	theme	cauline	865:871	arg1	length					847:852	length	847:852	length	847:852	For macrophenotyping, we provide dataset regarding (i) rosettes such as measurement of diameter and fresh mass as well as number of leaves just before bolting and (ii) floral stems at the first flower stage such as length, number of cauline leaves, mass and diameter at its base.
31489348	5	47	theme	monosaccharides	988:1002	arg1	quantification					964:977	quantification	964:977	quantification of seven monosaccharides and the reconstruction three polysaccharides	964:1047	Regarding cell wall composition, we provide data of quantification of seven monosaccharides and the reconstruction three polysaccharides.
31489348	3	48	theme	newly-described	510:524	arg1	ecotypes					535:542	four newly-described Pyrenees ecotypes	505:542	four newly-described Pyrenees ecotypes (Duruflé et al., 2019)	505:565	The well-described and sequenced Columbia (Col) ecotype and four newly-described Pyrenees ecotypes (Duruflé et al., 2019) have been grown at two different temperatures (15 °C and 22 °C).
31489348	3	48	theme	newly-described	510:524	arg1	Duruflé					545:551	Duruflé	545:551	Duruflé	545:551	The well-described and sequenced Columbia (Col) ecotype and four newly-described Pyrenees ecotypes (Duruflé et al., 2019) have been grown at two different temperatures (15 °C and 22 °C).
31489348	5	49	theme	wall	927:930	arg1	composition					932:942	cell wall composition	922:942	cell wall composition	922:942	Regarding cell wall composition, we provide data of quantification of seven monosaccharides and the reconstruction three polysaccharides.
31489348	3	50	theme	Pyrenees	526:533	arg1	ecotypes					535:542	four newly-described Pyrenees ecotypes	505:542	four newly-described Pyrenees ecotypes (Duruflé et al., 2019)	505:565	The well-described and sequenced Columbia (Col) ecotype and four newly-described Pyrenees ecotypes (Duruflé et al., 2019) have been grown at two different temperatures (15 °C and 22 °C).
31489348	3	50	theme	Pyrenees	526:533	arg1	Duruflé					545:551	Duruflé	545:551	Duruflé	545:551	The well-described and sequenced Columbia (Col) ecotype and four newly-described Pyrenees ecotypes (Duruflé et al., 2019) have been grown at two different temperatures (15 °C and 22 °C).
31489348	1	51	theme	rosettes	246:253	arg1	morphology					191:200	morphology	191:200	morphology	191:200	This article presents experimental data describing the morphology and the cell wall monosaccharide content of rosettes and flower stems of five Arabidopsis thaliana ecotypes grown at two contrasted temperatures.
31489348	1	51	theme	rosettes	246:253	arg1	content					235:241	the cell wall monosaccharide content	206:241	the cell wall monosaccharide content	206:241	This article presents experimental data describing the morphology and the cell wall monosaccharide content of rosettes and flower stems of five Arabidopsis thaliana ecotypes grown at two contrasted temperatures.
31489348	0	52	theme	arabidopsis	69:79	arg1	ecotypes					81:88	five arabidopsis ecotypes	64:88	five arabidopsis ecotypes	64:88	Phenotyping and cell wall polysaccharide composition dataset of five arabidopsis ecotypes grown at optimal or sub-optimal temperatures.
31489348	4	53	dep	stems	807:811	arg1	ii					796:797	ii	796:797	ii	796:797	For macrophenotyping, we provide dataset regarding (i) rosettes such as measurement of diameter and fresh mass as well as number of leaves just before bolting and (ii) floral stems at the first flower stage such as length, number of cauline leaves, mass and diameter at its base.
31489348	7	54	theme	growth	1276:1281	arg1	conditions					1283:1292	sub-optimal temperature growth conditions	1252:1292	sub-optimal temperature growth conditions	1252:1292	They constitute a valuable resource for the community to study the adaptation of A. thaliana ecotypes to sub-optimal temperature growth conditions.
31489348	1	55	theme	experimental	158:169	arg1	data					171:174	experimental data	158:174	experimental data describing the morphology and the cell wall monosaccharide content of rosettes and flower stems of five Arabidopsis thaliana ecotypes grown at two contrasted temperatures	158:345	This article presents experimental data describing the morphology and the cell wall monosaccharide content of rosettes and flower stems of five Arabidopsis thaliana ecotypes grown at two contrasted temperatures.
31489348	1	56	theme	flower	259:264	arg1	stems					266:270	flower stems	259:270	flower stems	259:270	This article presents experimental data describing the morphology and the cell wall monosaccharide content of rosettes and flower stems of five Arabidopsis thaliana ecotypes grown at two contrasted temperatures.
31825410	0	0	theme	targeted	120:127	arg1	therapy					129:135	targeted therapy	120:135	targeted therapy of hepatocellular carcinoma	120:163	Doxorubicin intercalated copper diethyldithiocarbamate functionalized layered double hydroxide hybrid nanoparticles for targeted therapy of hepatocellular carcinoma.
31825410	7	1	theme	glycol-graft-polyglutamic	1156:1180	arg1	acid					1182:1185	polyethylene glycol-graft-polyglutamic acid	1143:1185	polyethylene glycol-graft-polyglutamic acid (PEG-PLG) through electrostatic adsorption	1143:1228	The nanoparticles were coated with polyethylene glycol-graft-polyglutamic acid (PEG-PLG) through electrostatic adsorption to improve the stability of the nanoparticles.
31825410	7	1	theme	glycol-graft-polyglutamic	1156:1180	arg1	PEG-PLG					1188:1194	PEG-PLG	1188:1194	PEG-PLG	1188:1194	The nanoparticles were coated with polyethylene glycol-graft-polyglutamic acid (PEG-PLG) through electrostatic adsorption to improve the stability of the nanoparticles.
31825410	11	2	theme	cancer	1720:1725	arg1	therapy					1727:1733	synergistic cancer therapy	1708:1733	synergistic cancer therapy using the novel DOX intercalated Cu(DDC)2 functionalized layered double hydroxide hybrid nanoparticles	1708:1836	It can be concluded that there is a great potential for synergistic cancer therapy using the novel DOX intercalated Cu(DDC)2 functionalized layered double hydroxide hybrid nanoparticles.
31825410	3	3	theme	co-precipitation	525:540	arg1	method					542:547	the co-precipitation method	521:547	the co-precipitation method	521:547	In this research, we synthesized a hexagonal nanoparticle by the co-precipitation method, referred to as Cu-Al LDH.
31825410	1	4	theme	cure	271:274	arg1	rate					276:279	low cure rate	267:279	low cure rate	267:279	Hepatocellular carcinoma (HCC) is one of the deadliest cancers due to its long incubation period and low cure rate.
31825410	10	5	theme	ectopic	1618:1624	arg1	carcinoma					1641:1649	ectopic hepatocellular carcinoma	1618:1649	ectopic hepatocellular carcinoma	1618:1649	In addition, targeted nanoparticles effectively inhibited tumor growth in mouse models of ectopic hepatocellular carcinoma.
31825410	6	6	theme	nanoparticles	1034:1046	arg1	Formulations					987:998	Formulations	987:998	Formulations of the Cu(DDC)2 and DOX co-loaded nanoparticles	987:1046	Formulations of the Cu(DDC)2 and DOX co-loaded nanoparticles were optimized to precisely control the Cu(DDC)2/DOX ratio.
31825410	9	7	theme	greater	1448:1454	arg1	uptake					1465:1470	greater cellular uptake	1448:1470	greater cellular uptake	1448:1470	Compared with non-HA coated nanoparticles, HA coated nanoparticles showed greater cellular uptake in Hep G2 cells, which could cause higher cytotoxicity.
31825410	11	8	theme	novel	1745:1749	arg1	nanoparticles					1824:1836	the novel DOX intercalated Cu(DDC)2 functionalized layered double hydroxide hybrid nanoparticles	1741:1836	the novel DOX intercalated Cu(DDC)2 functionalized layered double hydroxide hybrid nanoparticles	1741:1836	It can be concluded that there is a great potential for synergistic cancer therapy using the novel DOX intercalated Cu(DDC)2 functionalized layered double hydroxide hybrid nanoparticles.
31825410	5	9	theme	treatment	968:976	arg1	relevance					951:959	the clinical relevance	938:959	the clinical relevance of DOX treatment for HCC	938:984	Doxorubicin (DOX) was also encapsulated into LDHs due to the clinical relevance of DOX treatment for HCC.
31825410	3	10	theme	Cu-Al	565:569	arg1	LDH					571:573	Cu-Al LDH	565:573	Cu-Al LDH	565:573	In this research, we synthesized a hexagonal nanoparticle by the co-precipitation method, referred to as Cu-Al LDH.
31825410	7	11	theme	polyethylene	1143:1154	arg1	acid					1182:1185	polyethylene glycol-graft-polyglutamic acid	1143:1185	polyethylene glycol-graft-polyglutamic acid (PEG-PLG) through electrostatic adsorption	1143:1228	The nanoparticles were coated with polyethylene glycol-graft-polyglutamic acid (PEG-PLG) through electrostatic adsorption to improve the stability of the nanoparticles.
31825410	7	11	theme	polyethylene	1143:1154	arg1	PEG-PLG					1188:1194	PEG-PLG	1188:1194	PEG-PLG	1188:1194	The nanoparticles were coated with polyethylene glycol-graft-polyglutamic acid (PEG-PLG) through electrostatic adsorption to improve the stability of the nanoparticles.
31825410	4	12	theme	cancer	865:870	arg1	therapy					872:878	cancer therapy	865:878	cancer therapy	865:878	As an alternative to traditional drug-loading methods, sodium diethyldithiocarbamate (DDC) was introduced and combined with Cu2+ in LDHs to form a diethyldithiocarbamate-copper complex (Cu(DDC)2), which was not only the composition of carrier materials but also an effective component for cancer therapy.
31825410	10	13	theme	tumor	1586:1590	arg1	growth					1592:1597	tumor growth	1586:1597	tumor growth	1586:1597	In addition, targeted nanoparticles effectively inhibited tumor growth in mouse models of ectopic hepatocellular carcinoma.
31825410	5	14	theme	clinical	942:949	arg1	relevance					951:959	the clinical relevance	938:959	the clinical relevance of DOX treatment for HCC	938:984	Doxorubicin (DOX) was also encapsulated into LDHs due to the clinical relevance of DOX treatment for HCC.
31825410	4	15	theme	drug-loading	609:620	arg1	methods					622:628	traditional drug-loading methods	597:628	traditional drug-loading methods	597:628	As an alternative to traditional drug-loading methods, sodium diethyldithiocarbamate (DDC) was introduced and combined with Cu2+ in LDHs to form a diethyldithiocarbamate-copper complex (Cu(DDC)2), which was not only the composition of carrier materials but also an effective component for cancer therapy.
31825410	0	16	theme	carcinoma	155:163	arg1	therapy					129:135	targeted therapy	120:135	targeted therapy of hepatocellular carcinoma	120:163	Doxorubicin intercalated copper diethyldithiocarbamate functionalized layered double hydroxide hybrid nanoparticles for targeted therapy of hepatocellular carcinoma.
31825410	6	17	theme	Cu	1088:1089	arg1	ratio					1101:1105	the Cu(DDC)2/DOX ratio	1084:1105	the Cu(DDC)2/DOX ratio	1084:1105	Formulations of the Cu(DDC)2 and DOX co-loaded nanoparticles were optimized to precisely control the Cu(DDC)2/DOX ratio.
31825410	9	18	theme	coated	1420:1425	arg1	nanoparticles					1427:1439	HA coated nanoparticles	1417:1439	HA coated nanoparticles	1417:1439	Compared with non-HA coated nanoparticles, HA coated nanoparticles showed greater cellular uptake in Hep G2 cells, which could cause higher cytotoxicity.
31825410	2	19	theme	hydroxide	297:305	arg1	nanoparticles					313:325	Layered double hydroxide (LDH) nanoparticles	282:325	Layered double hydroxide (LDH) nanoparticles	282:325	Layered double hydroxide (LDH) nanoparticles have attracted considerable research interest in the field of nanomedicine owing to their surface effects and good biocompatibility.
31825410	2	20	theme	surface	417:423	arg1	effects					425:431	their surface effects	411:431	their surface effects	411:431	Layered double hydroxide (LDH) nanoparticles have attracted considerable research interest in the field of nanomedicine owing to their surface effects and good biocompatibility.
31825410	0	21	theme	hepatocellular	140:153	arg1	carcinoma					155:163	hepatocellular carcinoma	140:163	hepatocellular carcinoma	140:163	Doxorubicin intercalated copper diethyldithiocarbamate functionalized layered double hydroxide hybrid nanoparticles for targeted therapy of hepatocellular carcinoma.
31825410	8	22	theme	outer	1281:1285	arg1	layer					1287:1291	The outer layer	1277:1291	The outer layer	1277:1291	The outer layer was decorated with hyaluronic acid (HA) to achieve specific targeting of tumors.
31825410	4	23	theme	traditional	597:607	arg1	methods					622:628	traditional drug-loading methods	597:628	traditional drug-loading methods	597:628	As an alternative to traditional drug-loading methods, sodium diethyldithiocarbamate (DDC) was introduced and combined with Cu2+ in LDHs to form a diethyldithiocarbamate-copper complex (Cu(DDC)2), which was not only the composition of carrier materials but also an effective component for cancer therapy.
31825410	2	24	theme	double	290:295	arg1	LDH					308:310	LDH	308:310	LDH	308:310	Layered double hydroxide (LDH) nanoparticles have attracted considerable research interest in the field of nanomedicine owing to their surface effects and good biocompatibility.
31825410	2	24	theme	double	290:295	arg1	hydroxide					297:305	Layered double hydroxide	282:305	Layered double hydroxide (LDH) nanoparticles	282:325	Layered double hydroxide (LDH) nanoparticles have attracted considerable research interest in the field of nanomedicine owing to their surface effects and good biocompatibility.
31825410	6	25	theme	DOX	1020:1022	arg1	nanoparticles					1034:1046	DOX co-loaded nanoparticles	1020:1046	DOX co-loaded nanoparticles	1020:1046	Formulations of the Cu(DDC)2 and DOX co-loaded nanoparticles were optimized to precisely control the Cu(DDC)2/DOX ratio.
31825410	0	26	theme	intercalated	12:23	arg1	diethyldithiocarbamate					32:53	Doxorubicin intercalated copper diethyldithiocarbamate	0:53	Doxorubicin intercalated copper diethyldithiocarbamate	0:53	Doxorubicin intercalated copper diethyldithiocarbamate functionalized layered double hydroxide hybrid nanoparticles for targeted therapy of hepatocellular carcinoma.
31825410	8	27	theme	tumors	1366:1371	arg1	targeting					1353:1361	specific targeting	1344:1361	specific targeting of tumors	1344:1371	The outer layer was decorated with hyaluronic acid (HA) to achieve specific targeting of tumors.
31825410	4	28	theme	effective	841:849	arg1	component					851:859	an effective component	838:859	not only the composition of carrier materials but also an effective component for cancer therapy	783:878	As an alternative to traditional drug-loading methods, sodium diethyldithiocarbamate (DDC) was introduced and combined with Cu2+ in LDHs to form a diethyldithiocarbamate-copper complex (Cu(DDC)2), which was not only the composition of carrier materials but also an effective component for cancer therapy.
31825410	9	29	theme	higher	1507:1512	arg1	cytotoxicity					1514:1525	higher cytotoxicity	1507:1525	higher cytotoxicity	1507:1525	Compared with non-HA coated nanoparticles, HA coated nanoparticles showed greater cellular uptake in Hep G2 cells, which could cause higher cytotoxicity.
31825410	6	30	theme	2/DOX	1095:1099	arg1	ratio					1101:1105	the Cu(DDC)2/DOX ratio	1084:1105	the Cu(DDC)2/DOX ratio	1084:1105	Formulations of the Cu(DDC)2 and DOX co-loaded nanoparticles were optimized to precisely control the Cu(DDC)2/DOX ratio.
31825410	11	31	theme	double	1800:1805	arg1	nanoparticles					1824:1836	the novel DOX intercalated Cu(DDC)2 functionalized layered double hydroxide hybrid nanoparticles	1741:1836	the novel DOX intercalated Cu(DDC)2 functionalized layered double hydroxide hybrid nanoparticles	1741:1836	It can be concluded that there is a great potential for synergistic cancer therapy using the novel DOX intercalated Cu(DDC)2 functionalized layered double hydroxide hybrid nanoparticles.
31825410	1	32	theme	deadliest	211:219	arg1	cancers					221:227	the deadliest cancers	207:227	the deadliest cancers	207:227	Hepatocellular carcinoma (HCC) is one of the deadliest cancers due to its long incubation period and low cure rate.
31825410	0	33	theme	Doxorubicin	0:10	arg1	diethyldithiocarbamate					32:53	Doxorubicin intercalated copper diethyldithiocarbamate	0:53	Doxorubicin intercalated copper diethyldithiocarbamate	0:53	Doxorubicin intercalated copper diethyldithiocarbamate functionalized layered double hydroxide hybrid nanoparticles for targeted therapy of hepatocellular carcinoma.
31825410	11	34	theme	Cu	1768:1769	arg1	nanoparticles					1824:1836	the novel DOX intercalated Cu(DDC)2 functionalized layered double hydroxide hybrid nanoparticles	1741:1836	the novel DOX intercalated Cu(DDC)2 functionalized layered double hydroxide hybrid nanoparticles	1741:1836	It can be concluded that there is a great potential for synergistic cancer therapy using the novel DOX intercalated Cu(DDC)2 functionalized layered double hydroxide hybrid nanoparticles.
31825410	11	35	theme	great	1688:1692	arg1	potential					1694:1702	a great potential	1686:1702	a great potential for synergistic cancer therapy using the novel DOX intercalated Cu(DDC)2 functionalized layered double hydroxide hybrid nanoparticles	1686:1836	It can be concluded that there is a great potential for synergistic cancer therapy using the novel DOX intercalated Cu(DDC)2 functionalized layered double hydroxide hybrid nanoparticles.
31825410	4	36	theme	Cu	762:763	arg1	DDC					765:767	Cu(DDC)2	762:769	Cu(DDC)2	762:769	As an alternative to traditional drug-loading methods, sodium diethyldithiocarbamate (DDC) was introduced and combined with Cu2+ in LDHs to form a diethyldithiocarbamate-copper complex (Cu(DDC)2), which was not only the composition of carrier materials but also an effective component for cancer therapy.
31825410	4	36	theme	Cu	762:763	arg1	complex					753:759	a diethyldithiocarbamate-copper complex	721:759	a diethyldithiocarbamate-copper complex (Cu(DDC)2)	721:770	As an alternative to traditional drug-loading methods, sodium diethyldithiocarbamate (DDC) was introduced and combined with Cu2+ in LDHs to form a diethyldithiocarbamate-copper complex (Cu(DDC)2), which was not only the composition of carrier materials but also an effective component for cancer therapy.
31825410	1	37	theme	cancers	221:227	arg1	cancers					221:227	the deadliest cancers	207:227	the deadliest cancers	207:227	Hepatocellular carcinoma (HCC) is one of the deadliest cancers due to its long incubation period and low cure rate.
31825410	1	37	theme	cancers	221:227	arg1	one					200:202	one	200:202	one	200:202	Hepatocellular carcinoma (HCC) is one of the deadliest cancers due to its long incubation period and low cure rate.
31825410	10	38	theme	targeted	1541:1548	arg1	nanoparticles					1550:1562	targeted nanoparticles	1541:1562	targeted nanoparticles	1541:1562	In addition, targeted nanoparticles effectively inhibited tumor growth in mouse models of ectopic hepatocellular carcinoma.
31825410	9	39	theme	non-HA	1388:1393	arg1	nanoparticles					1402:1414	non-HA coated nanoparticles	1388:1414	non-HA coated nanoparticles	1388:1414	Compared with non-HA coated nanoparticles, HA coated nanoparticles showed greater cellular uptake in Hep G2 cells, which could cause higher cytotoxicity.
31825410	5	40	theme	due	931:933	arg1	LDHs					926:929	LDHs	926:929	LDHs due to the clinical relevance of DOX treatment for HCC	926:984	Doxorubicin (DOX) was also encapsulated into LDHs due to the clinical relevance of DOX treatment for HCC.
31825410	7	41	theme	nanoparticles	1262:1274	arg1	stability					1245:1253	the stability	1241:1253	the stability of the nanoparticles	1241:1274	The nanoparticles were coated with polyethylene glycol-graft-polyglutamic acid (PEG-PLG) through electrostatic adsorption to improve the stability of the nanoparticles.
31825410	0	42	theme	copper	25:30	arg1	diethyldithiocarbamate					32:53	Doxorubicin intercalated copper diethyldithiocarbamate	0:53	Doxorubicin intercalated copper diethyldithiocarbamate	0:53	Doxorubicin intercalated copper diethyldithiocarbamate functionalized layered double hydroxide hybrid nanoparticles for targeted therapy of hepatocellular carcinoma.
31825410	11	43	theme	DOX	1751:1753	arg1	nanoparticles					1824:1836	the novel DOX intercalated Cu(DDC)2 functionalized layered double hydroxide hybrid nanoparticles	1741:1836	the novel DOX intercalated Cu(DDC)2 functionalized layered double hydroxide hybrid nanoparticles	1741:1836	It can be concluded that there is a great potential for synergistic cancer therapy using the novel DOX intercalated Cu(DDC)2 functionalized layered double hydroxide hybrid nanoparticles.
31825410	2	44	theme	good	437:440	arg1	biocompatibility					442:457	good biocompatibility	437:457	good biocompatibility	437:457	Layered double hydroxide (LDH) nanoparticles have attracted considerable research interest in the field of nanomedicine owing to their surface effects and good biocompatibility.
31825410	0	45	theme	layered	70:76	arg1	nanoparticles					102:114	layered double hydroxide hybrid nanoparticles	70:114	layered double hydroxide hybrid nanoparticles for targeted therapy of hepatocellular carcinoma	70:163	Doxorubicin intercalated copper diethyldithiocarbamate functionalized layered double hydroxide hybrid nanoparticles for targeted therapy of hepatocellular carcinoma.
31825410	9	46	theme	cellular	1456:1463	arg1	uptake					1465:1470	greater cellular uptake	1448:1470	greater cellular uptake	1448:1470	Compared with non-HA coated nanoparticles, HA coated nanoparticles showed greater cellular uptake in Hep G2 cells, which could cause higher cytotoxicity.
31825410	11	47	theme	hydroxide	1807:1815	arg1	nanoparticles					1824:1836	the novel DOX intercalated Cu(DDC)2 functionalized layered double hydroxide hybrid nanoparticles	1741:1836	the novel DOX intercalated Cu(DDC)2 functionalized layered double hydroxide hybrid nanoparticles	1741:1836	It can be concluded that there is a great potential for synergistic cancer therapy using the novel DOX intercalated Cu(DDC)2 functionalized layered double hydroxide hybrid nanoparticles.
31825410	7	48	theme	electrostatic	1205:1217	arg1	adsorption					1219:1228	electrostatic adsorption	1205:1228	electrostatic adsorption	1205:1228	The nanoparticles were coated with polyethylene glycol-graft-polyglutamic acid (PEG-PLG) through electrostatic adsorption to improve the stability of the nanoparticles.
31825410	8	49	theme	specific	1344:1351	arg1	targeting					1353:1361	specific targeting	1344:1361	specific targeting of tumors	1344:1371	The outer layer was decorated with hyaluronic acid (HA) to achieve specific targeting of tumors.
31825410	10	50	theme	mouse	1602:1606	arg1	models					1608:1613	mouse models	1602:1613	mouse models of ectopic hepatocellular carcinoma	1602:1649	In addition, targeted nanoparticles effectively inhibited tumor growth in mouse models of ectopic hepatocellular carcinoma.
31825410	10	51	theme	carcinoma	1641:1649	arg1	models					1608:1613	mouse models	1602:1613	mouse models of ectopic hepatocellular carcinoma	1602:1649	In addition, targeted nanoparticles effectively inhibited tumor growth in mouse models of ectopic hepatocellular carcinoma.
31825410	6	52	theme	co-loaded	1024:1032	arg1	nanoparticles					1034:1046	DOX co-loaded nanoparticles	1020:1046	DOX co-loaded nanoparticles	1020:1046	Formulations of the Cu(DDC)2 and DOX co-loaded nanoparticles were optimized to precisely control the Cu(DDC)2/DOX ratio.
31825410	1	53	theme	long	240:243	arg1	period					256:261	its long incubation period	236:261	its long incubation period	236:261	Hepatocellular carcinoma (HCC) is one of the deadliest cancers due to its long incubation period and low cure rate.
31825410	0	54	theme	hydroxide	85:93	arg1	nanoparticles					102:114	layered double hydroxide hybrid nanoparticles	70:114	layered double hydroxide hybrid nanoparticles for targeted therapy of hepatocellular carcinoma	70:163	Doxorubicin intercalated copper diethyldithiocarbamate functionalized layered double hydroxide hybrid nanoparticles for targeted therapy of hepatocellular carcinoma.
31825410	11	55	theme	functionalized	1777:1790	arg1	nanoparticles					1824:1836	the novel DOX intercalated Cu(DDC)2 functionalized layered double hydroxide hybrid nanoparticles	1741:1836	the novel DOX intercalated Cu(DDC)2 functionalized layered double hydroxide hybrid nanoparticles	1741:1836	It can be concluded that there is a great potential for synergistic cancer therapy using the novel DOX intercalated Cu(DDC)2 functionalized layered double hydroxide hybrid nanoparticles.
31825410	4	56	theme	materials	819:827	arg1	complex					753:759	a diethyldithiocarbamate-copper complex	721:759	a diethyldithiocarbamate-copper complex (Cu(DDC)2)	721:770	As an alternative to traditional drug-loading methods, sodium diethyldithiocarbamate (DDC) was introduced and combined with Cu2+ in LDHs to form a diethyldithiocarbamate-copper complex (Cu(DDC)2), which was not only the composition of carrier materials but also an effective component for cancer therapy.
31825410	4	56	theme	materials	819:827	arg1	component					851:859	an effective component	838:859	not only the composition of carrier materials but also an effective component for cancer therapy	783:878	As an alternative to traditional drug-loading methods, sodium diethyldithiocarbamate (DDC) was introduced and combined with Cu2+ in LDHs to form a diethyldithiocarbamate-copper complex (Cu(DDC)2), which was not only the composition of carrier materials but also an effective component for cancer therapy.
31825410	4	56	theme	materials	819:827	arg1	composition					796:806	the composition	792:806	not only the composition of carrier materials but also an effective component for cancer therapy	783:878	As an alternative to traditional drug-loading methods, sodium diethyldithiocarbamate (DDC) was introduced and combined with Cu2+ in LDHs to form a diethyldithiocarbamate-copper complex (Cu(DDC)2), which was not only the composition of carrier materials but also an effective component for cancer therapy.
31825410	4	57	theme	diethyldithiocarbamate-copper	723:751	arg1	DDC					765:767	Cu(DDC)2	762:769	Cu(DDC)2	762:769	As an alternative to traditional drug-loading methods, sodium diethyldithiocarbamate (DDC) was introduced and combined with Cu2+ in LDHs to form a diethyldithiocarbamate-copper complex (Cu(DDC)2), which was not only the composition of carrier materials but also an effective component for cancer therapy.
31825410	4	57	theme	diethyldithiocarbamate-copper	723:751	arg1	complex					753:759	a diethyldithiocarbamate-copper complex	721:759	a diethyldithiocarbamate-copper complex (Cu(DDC)2)	721:770	As an alternative to traditional drug-loading methods, sodium diethyldithiocarbamate (DDC) was introduced and combined with Cu2+ in LDHs to form a diethyldithiocarbamate-copper complex (Cu(DDC)2), which was not only the composition of carrier materials but also an effective component for cancer therapy.
31825410	4	57	theme	diethyldithiocarbamate-copper	723:751	arg1	composition					796:806	the composition	792:806	not only the composition of carrier materials but also an effective component for cancer therapy	783:878	As an alternative to traditional drug-loading methods, sodium diethyldithiocarbamate (DDC) was introduced and combined with Cu2+ in LDHs to form a diethyldithiocarbamate-copper complex (Cu(DDC)2), which was not only the composition of carrier materials but also an effective component for cancer therapy.
31825410	0	58	theme	double	78:83	arg1	nanoparticles					102:114	layered double hydroxide hybrid nanoparticles	70:114	layered double hydroxide hybrid nanoparticles for targeted therapy of hepatocellular carcinoma	70:163	Doxorubicin intercalated copper diethyldithiocarbamate functionalized layered double hydroxide hybrid nanoparticles for targeted therapy of hepatocellular carcinoma.
31825410	11	59	theme	DDC	1771:1773	arg1	nanoparticles					1824:1836	the novel DOX intercalated Cu(DDC)2 functionalized layered double hydroxide hybrid nanoparticles	1741:1836	the novel DOX intercalated Cu(DDC)2 functionalized layered double hydroxide hybrid nanoparticles	1741:1836	It can be concluded that there is a great potential for synergistic cancer therapy using the novel DOX intercalated Cu(DDC)2 functionalized layered double hydroxide hybrid nanoparticles.
31825410	4	60	theme	carrier	811:817	arg1	materials					819:827	carrier materials	811:827	carrier materials	811:827	As an alternative to traditional drug-loading methods, sodium diethyldithiocarbamate (DDC) was introduced and combined with Cu2+ in LDHs to form a diethyldithiocarbamate-copper complex (Cu(DDC)2), which was not only the composition of carrier materials but also an effective component for cancer therapy.
31825410	11	61	theme	synergistic	1708:1718	arg1	therapy					1727:1733	synergistic cancer therapy	1708:1733	synergistic cancer therapy using the novel DOX intercalated Cu(DDC)2 functionalized layered double hydroxide hybrid nanoparticles	1708:1836	It can be concluded that there is a great potential for synergistic cancer therapy using the novel DOX intercalated Cu(DDC)2 functionalized layered double hydroxide hybrid nanoparticles.
31825410	1	62	theme	Hepatocellular	166:179	arg1	carcinoma					181:189	Hepatocellular carcinoma	166:189	Hepatocellular carcinoma (HCC)	166:195	Hepatocellular carcinoma (HCC) is one of the deadliest cancers due to its long incubation period and low cure rate.
31825410	1	62	theme	Hepatocellular	166:179	arg1	HCC					192:194	HCC	192:194	HCC	192:194	Hepatocellular carcinoma (HCC) is one of the deadliest cancers due to its long incubation period and low cure rate.
31825410	11	63	theme	hybrid	1817:1822	arg1	nanoparticles					1824:1836	the novel DOX intercalated Cu(DDC)2 functionalized layered double hydroxide hybrid nanoparticles	1741:1836	the novel DOX intercalated Cu(DDC)2 functionalized layered double hydroxide hybrid nanoparticles	1741:1836	It can be concluded that there is a great potential for synergistic cancer therapy using the novel DOX intercalated Cu(DDC)2 functionalized layered double hydroxide hybrid nanoparticles.
31825410	5	64	theme	DOX	964:966	arg1	treatment					968:976	DOX treatment	964:976	DOX treatment	964:976	Doxorubicin (DOX) was also encapsulated into LDHs due to the clinical relevance of DOX treatment for HCC.
31825410	2	65	theme	Layered	282:288	arg1	LDH					308:310	LDH	308:310	LDH	308:310	Layered double hydroxide (LDH) nanoparticles have attracted considerable research interest in the field of nanomedicine owing to their surface effects and good biocompatibility.
31825410	2	65	theme	Layered	282:288	arg1	hydroxide					297:305	Layered double hydroxide	282:305	Layered double hydroxide (LDH) nanoparticles	282:325	Layered double hydroxide (LDH) nanoparticles have attracted considerable research interest in the field of nanomedicine owing to their surface effects and good biocompatibility.
31825410	9	66	theme	G2	1479:1480	arg1	cells					1482:1486	Hep G2 cells	1475:1486	Hep G2 cells	1475:1486	Compared with non-HA coated nanoparticles, HA coated nanoparticles showed greater cellular uptake in Hep G2 cells, which could cause higher cytotoxicity.
31825410	2	67	theme	research	355:362	arg1	interest					364:371	considerable research interest	342:371	considerable research interest	342:371	Layered double hydroxide (LDH) nanoparticles have attracted considerable research interest in the field of nanomedicine owing to their surface effects and good biocompatibility.
31825410	1	68	theme	incubation	245:254	arg1	period					256:261	its long incubation period	236:261	its long incubation period	236:261	Hepatocellular carcinoma (HCC) is one of the deadliest cancers due to its long incubation period and low cure rate.
31825410	8	69	theme	hyaluronic	1312:1321	arg1	HA					1329:1330	HA	1329:1330	HA	1329:1330	The outer layer was decorated with hyaluronic acid (HA) to achieve specific targeting of tumors.
31825410	8	69	theme	hyaluronic	1312:1321	arg1	acid					1323:1326	hyaluronic acid	1312:1326	hyaluronic acid (HA)	1312:1331	The outer layer was decorated with hyaluronic acid (HA) to achieve specific targeting of tumors.
31825410	11	70	theme	intercalated	1755:1766	arg1	nanoparticles					1824:1836	the novel DOX intercalated Cu(DDC)2 functionalized layered double hydroxide hybrid nanoparticles	1741:1836	the novel DOX intercalated Cu(DDC)2 functionalized layered double hydroxide hybrid nanoparticles	1741:1836	It can be concluded that there is a great potential for synergistic cancer therapy using the novel DOX intercalated Cu(DDC)2 functionalized layered double hydroxide hybrid nanoparticles.
31825410	4	71	dep	form	716:719	arg1	LDHs					708:711	LDHs	708:711	LDHs	708:711	As an alternative to traditional drug-loading methods, sodium diethyldithiocarbamate (DDC) was introduced and combined with Cu2+ in LDHs to form a diethyldithiocarbamate-copper complex (Cu(DDC)2), which was not only the composition of carrier materials but also an effective component for cancer therapy.
31825410	10	72	theme	hepatocellular	1626:1639	arg1	carcinoma					1641:1649	ectopic hepatocellular carcinoma	1618:1649	ectopic hepatocellular carcinoma	1618:1649	In addition, targeted nanoparticles effectively inhibited tumor growth in mouse models of ectopic hepatocellular carcinoma.
31825410	2	73	theme	considerable	342:353	arg1	interest					364:371	considerable research interest	342:371	considerable research interest	342:371	Layered double hydroxide (LDH) nanoparticles have attracted considerable research interest in the field of nanomedicine owing to their surface effects and good biocompatibility.
31825410	3	74	theme	hexagonal	495:503	arg1	nanoparticle					505:516	a hexagonal nanoparticle	493:516	a hexagonal nanoparticle	493:516	In this research, we synthesized a hexagonal nanoparticle by the co-precipitation method, referred to as Cu-Al LDH.
31825410	6	75	theme	Cu	1007:1008	arg1	Formulations					987:998	Formulations	987:998	Formulations of the Cu(DDC)2 and DOX co-loaded nanoparticles	987:1046	Formulations of the Cu(DDC)2 and DOX co-loaded nanoparticles were optimized to precisely control the Cu(DDC)2/DOX ratio.
31825410	9	76	theme	Hep	1475:1477	arg1	cells					1482:1486	Hep G2 cells	1475:1486	Hep G2 cells	1475:1486	Compared with non-HA coated nanoparticles, HA coated nanoparticles showed greater cellular uptake in Hep G2 cells, which could cause higher cytotoxicity.
31825410	2	77	theme	nanomedicine	389:400	arg1	field					380:384	the field	376:384	the field of nanomedicine owing to their surface effects and good biocompatibility	376:457	Layered double hydroxide (LDH) nanoparticles have attracted considerable research interest in the field of nanomedicine owing to their surface effects and good biocompatibility.
31825410	9	78	theme	coated	1395:1400	arg1	nanoparticles					1402:1414	non-HA coated nanoparticles	1388:1414	non-HA coated nanoparticles	1388:1414	Compared with non-HA coated nanoparticles, HA coated nanoparticles showed greater cellular uptake in Hep G2 cells, which could cause higher cytotoxicity.
31825410	0	79	theme	hybrid	95:100	arg1	nanoparticles					102:114	layered double hydroxide hybrid nanoparticles	70:114	layered double hydroxide hybrid nanoparticles for targeted therapy of hepatocellular carcinoma	70:163	Doxorubicin intercalated copper diethyldithiocarbamate functionalized layered double hydroxide hybrid nanoparticles for targeted therapy of hepatocellular carcinoma.
31825410	4	80	theme	sodium	631:636	arg1	DDC					662:664	DDC	662:664	DDC	662:664	As an alternative to traditional drug-loading methods, sodium diethyldithiocarbamate (DDC) was introduced and combined with Cu2+ in LDHs to form a diethyldithiocarbamate-copper complex (Cu(DDC)2), which was not only the composition of carrier materials but also an effective component for cancer therapy.
31825410	4	80	theme	sodium	631:636	arg1	diethyldithiocarbamate					638:659	sodium diethyldithiocarbamate	631:659	sodium diethyldithiocarbamate (DDC)	631:665	As an alternative to traditional drug-loading methods, sodium diethyldithiocarbamate (DDC) was introduced and combined with Cu2+ in LDHs to form a diethyldithiocarbamate-copper complex (Cu(DDC)2), which was not only the composition of carrier materials but also an effective component for cancer therapy.
31825410	4	80	theme	sodium	631:636	arg1	alternative					582:592	an alternative	579:592	an alternative to traditional drug-loading methods	579:628	As an alternative to traditional drug-loading methods, sodium diethyldithiocarbamate (DDC) was introduced and combined with Cu2+ in LDHs to form a diethyldithiocarbamate-copper complex (Cu(DDC)2), which was not only the composition of carrier materials but also an effective component for cancer therapy.
31825410	11	81	theme	layered	1792:1798	arg1	nanoparticles					1824:1836	the novel DOX intercalated Cu(DDC)2 functionalized layered double hydroxide hybrid nanoparticles	1741:1836	the novel DOX intercalated Cu(DDC)2 functionalized layered double hydroxide hybrid nanoparticles	1741:1836	It can be concluded that there is a great potential for synergistic cancer therapy using the novel DOX intercalated Cu(DDC)2 functionalized layered double hydroxide hybrid nanoparticles.
31825410	1	82	theme	low	267:269	arg1	rate					276:279	low cure rate	267:279	low cure rate	267:279	Hepatocellular carcinoma (HCC) is one of the deadliest cancers due to its long incubation period and low cure rate.
29958098	4	0	theme	symptoms	868:875	arg1	monitoring					846:855	Daily monitoring	840:855	Daily monitoring of disease symptoms (abnormal behavior, diarrhea, etc.) and body weights	840:928	Daily monitoring of disease symptoms (abnormal behavior, diarrhea, etc.) and body weights was undertaken.
29958098	1	1	theme	colitis	440:446	arg1	model					401:405	a mouse model	393:405	a mouse model of Citrobacter rodentium-induced colitis	393:446	The efficacy of two strains of Lactobacillus probiotics (Lactobacillus rhamnosus R0011 and Lactobacillus helveticus R0052) immobilized in microcapsules composed of pea protein isolate (PPI) and alginate microcapsules was assessed using a mouse model of Citrobacter rodentium-induced colitis.
29958098	4	2	theme	abnormal	878:885	arg1	diarrhea					897:904	diarrhea	897:904	diarrhea	897:904	Daily monitoring of disease symptoms (abnormal behavior, diarrhea, etc.) and body weights was undertaken.
29958098	4	2	theme	abnormal	878:885	arg1	behavior					887:894	abnormal behavior	878:894	abnormal behavior	878:894	Daily monitoring of disease symptoms (abnormal behavior, diarrhea, etc.) and body weights was undertaken.
29958098	4	2	theme	abnormal	878:885	arg1	etc.					907:910	etc.	907:910	etc.	907:910	Daily monitoring of disease symptoms (abnormal behavior, diarrhea, etc.) and body weights was undertaken.
29958098	6	3	theme	colitis	1259:1265	arg1	progression					1233:1243	marked progression	1226:1243	marked progression of infectious colitis	1226:1265	Infection with C. rodentium led to marked progression of infectious colitis, as revealed by symptomatic and histopathological data, changes in cytokine expression, and alteration of composition of mucosal communities.
29958098	7	4	from	profiles	1516:1523	arg1	animals					1537:1543	infected animals	1528:1543	infected animals	1528:1543	Probiotics led to changes in most of the disease markers but did not have a significant impact on cytokine profiles in infected animals.
29958098	7	5	theme	disease	1450:1456	arg1	markers					1458:1464	the disease markers	1446:1464	the disease markers	1446:1464	Probiotics led to changes in most of the disease markers but did not have a significant impact on cytokine profiles in infected animals.
29958098	4	6	theme	Daily	840:844	arg1	monitoring					846:855	Daily monitoring	840:855	Daily monitoring of disease symptoms (abnormal behavior, diarrhea, etc.) and body weights	840:928	Daily monitoring of disease symptoms (abnormal behavior, diarrhea, etc.) and body weights was undertaken.
29958098	1	7	theme	Lactobacillus	188:200	arg1	probiotics					202:211	Lactobacillus probiotics	188:211	Lactobacillus probiotics (Lactobacillus rhamnosus R0011 and Lactobacillus helveticus R0052) immobilized in microcapsules composed of pea protein isolate (PPI) and alginate microcapsules	188:372	The efficacy of two strains of Lactobacillus probiotics (Lactobacillus rhamnosus R0011 and Lactobacillus helveticus R0052) immobilized in microcapsules composed of pea protein isolate (PPI) and alginate microcapsules was assessed using a mouse model of Citrobacter rodentium-induced colitis.
29958098	1	7	theme	Lactobacillus	188:200	arg1	helveticus					262:271	Lactobacillus helveticus R0052	248:277	Lactobacillus helveticus R0052	248:277	The efficacy of two strains of Lactobacillus probiotics (Lactobacillus rhamnosus R0011 and Lactobacillus helveticus R0052) immobilized in microcapsules composed of pea protein isolate (PPI) and alginate microcapsules was assessed using a mouse model of Citrobacter rodentium-induced colitis.
29958098	1	7	theme	Lactobacillus	188:200	arg1	Lactobacillus					214:226	Lactobacillus	214:226	Lactobacillus	214:226	The efficacy of two strains of Lactobacillus probiotics (Lactobacillus rhamnosus R0011 and Lactobacillus helveticus R0052) immobilized in microcapsules composed of pea protein isolate (PPI) and alginate microcapsules was assessed using a mouse model of Citrobacter rodentium-induced colitis.
29958098	6	8	theme	marked	1226:1231	arg1	progression					1233:1243	marked progression	1226:1243	marked progression of infectious colitis	1226:1265	Infection with C. rodentium led to marked progression of infectious colitis, as revealed by symptomatic and histopathological data, changes in cytokine expression, and alteration of composition of mucosal communities.
29958098	5	9	from	levels	1022:1027	arg1	tissues					993:999	colonic and cecal tissues	975:999	colonic and cecal tissues	975:999	Histopathological changes in colonic and cecal tissues, cytokine expression levels, and pathogen and probiotic densities in feces were examined, and the microbial communities of the distal colon mucosa were characterized by 16S rRNA sequencing.
29958098	5	9	from	levels	1022:1027	arg1	feces					1070:1074	feces	1070:1074	feces	1070:1074	Histopathological changes in colonic and cecal tissues, cytokine expression levels, and pathogen and probiotic densities in feces were examined, and the microbial communities of the distal colon mucosa were characterized by 16S rRNA sequencing.
29958098	4	10	theme	body	917:920	arg1	weights					922:928	body weights	917:928	body weights	917:928	Daily monitoring of disease symptoms (abnormal behavior, diarrhea, etc.) and body weights was undertaken.
29958098	9	11	from	development	1899:1909	arg1	models					1965:1970	certain animal disease models	1942:1970	certain animal disease models	1942:1970	These results suggest that even though food ingredients may be generally recognized as safe, they may in fact contribute to the development of an inflammatory response in certain animal disease models.
29958098	3	12	theme	C.	780:781	arg1	rodentium					783:791	C. rodentium	780:791	C. rodentium	780:791	Half of the mice (controls, groups P, PE, and E) received C. rodentium by gavage 2 weeks after initiation of feeding.
29958098	1	13	theme	rodentium-induced	422:438	arg1	colitis					440:446	Citrobacter rodentium-induced colitis	410:446	Citrobacter rodentium-induced colitis	410:446	The efficacy of two strains of Lactobacillus probiotics (Lactobacillus rhamnosus R0011 and Lactobacillus helveticus R0052) immobilized in microcapsules composed of pea protein isolate (PPI) and alginate microcapsules was assessed using a mouse model of Citrobacter rodentium-induced colitis.
29958098	9	14	theme	certain	1942:1948	arg1	models					1965:1970	certain animal disease models	1942:1970	certain animal disease models	1942:1970	These results suggest that even though food ingredients may be generally recognized as safe, they may in fact contribute to the development of an inflammatory response in certain animal disease models.
29958098	0	15	from	signs	77:81	arg1	mice					127:130	mice	127:130	mice treated with probiotics	127:154	Pea-protein alginate encapsulation adversely affects development of clinical signs of Citrobacter rodentium-induced colitis in mice treated with probiotics.
29958098	9	16	theme	inflammatory	1917:1928	arg1	response					1930:1937	an inflammatory response	1914:1937	an inflammatory response in certain animal disease models	1914:1970	These results suggest that even though food ingredients may be generally recognized as safe, they may in fact contribute to the development of an inflammatory response in certain animal disease models.
29958098	8	17	theme	symptoms	1748:1755	arg1	symptoms					1748:1755	symptoms	1748:1755	symptoms	1748:1755	On the basis of cytokine expression analyses and histopathological data, it was evident that encapsulation materials (pea protein and calcium alginate) contributed to inflammation and worsened a set of symptoms in the cecum.
29958098	8	17	theme	symptoms	1748:1755	arg1	set					1741:1743	a set	1739:1743	a set of symptoms	1739:1755	On the basis of cytokine expression analyses and histopathological data, it was evident that encapsulation materials (pea protein and calcium alginate) contributed to inflammation and worsened a set of symptoms in the cecum.
29958098	2	18	theme	PPI-alginate	605:616	arg1	microcapsules					618:630	lyophilized PPI-alginate microcapsules	593:630	lyophilized PPI-alginate microcapsules containing probiotics	593:652	Accordingly, 4-week-old mice were fed diets supplemented with freeze-dried probiotics (group P), probiotic-containing microcapsules (group PE) (lyophilized PPI-alginate microcapsules containing probiotics), or PPI-alginate microcapsules containing no probiotics (group E).
29958098	0	19	from	mice	127:130	arg1	signs					77:81	clinical signs	68:81	clinical signs of Citrobacter rodentium-induced colitis in mice treated with probiotics	68:154	Pea-protein alginate encapsulation adversely affects development of clinical signs of Citrobacter rodentium-induced colitis in mice treated with probiotics.
29958098	0	20	from	colitis	116:122	arg1	mice					127:130	mice	127:130	mice treated with probiotics	127:154	Pea-protein alginate encapsulation adversely affects development of clinical signs of Citrobacter rodentium-induced colitis in mice treated with probiotics.
29958098	9	21	theme	animal	1950:1955	arg1	models					1965:1970	certain animal disease models	1942:1970	certain animal disease models	1942:1970	These results suggest that even though food ingredients may be generally recognized as safe, they may in fact contribute to the development of an inflammatory response in certain animal disease models.
29958098	6	22	theme	composition	1373:1383	arg1	changes					1323:1329	changes	1323:1329	changes in cytokine expression	1323:1352	Infection with C. rodentium led to marked progression of infectious colitis, as revealed by symptomatic and histopathological data, changes in cytokine expression, and alteration of composition of mucosal communities.
29958098	6	22	theme	composition	1373:1383	arg1	alteration					1359:1368	alteration	1359:1368	alteration of composition of mucosal communities	1359:1406	Infection with C. rodentium led to marked progression of infectious colitis, as revealed by symptomatic and histopathological data, changes in cytokine expression, and alteration of composition of mucosal communities.
29958098	6	22	theme	composition	1373:1383	arg1	data					1317:1320	symptomatic and histopathological data	1283:1320	symptomatic and histopathological data	1283:1320	Infection with C. rodentium led to marked progression of infectious colitis, as revealed by symptomatic and histopathological data, changes in cytokine expression, and alteration of composition of mucosal communities.
29958098	5	23	theme	microbial	1099:1107	arg1	communities					1109:1119	the microbial communities	1095:1119	the microbial communities of the distal colon mucosa	1095:1146	Histopathological changes in colonic and cecal tissues, cytokine expression levels, and pathogen and probiotic densities in feces were examined, and the microbial communities of the distal colon mucosa were characterized by 16S rRNA sequencing.
29958098	2	24	theme	group	536:540	arg1	P					542:542	group P	536:542	group P	536:542	Accordingly, 4-week-old mice were fed diets supplemented with freeze-dried probiotics (group P), probiotic-containing microcapsules (group PE) (lyophilized PPI-alginate microcapsules containing probiotics), or PPI-alginate microcapsules containing no probiotics (group E).
29958098	2	24	theme	group	536:540	arg1	probiotics					524:533	freeze-dried probiotics	511:533	freeze-dried probiotics (group P)	511:543	Accordingly, 4-week-old mice were fed diets supplemented with freeze-dried probiotics (group P), probiotic-containing microcapsules (group PE) (lyophilized PPI-alginate microcapsules containing probiotics), or PPI-alginate microcapsules containing no probiotics (group E).
29958098	1	25	theme	protein	325:331	arg1	PPI					342:344	PPI	342:344	PPI	342:344	The efficacy of two strains of Lactobacillus probiotics (Lactobacillus rhamnosus R0011 and Lactobacillus helveticus R0052) immobilized in microcapsules composed of pea protein isolate (PPI) and alginate microcapsules was assessed using a mouse model of Citrobacter rodentium-induced colitis.
29958098	1	25	theme	protein	325:331	arg1	isolate					333:339	pea protein isolate	321:339	pea protein isolate (PPI)	321:345	The efficacy of two strains of Lactobacillus probiotics (Lactobacillus rhamnosus R0011 and Lactobacillus helveticus R0052) immobilized in microcapsules composed of pea protein isolate (PPI) and alginate microcapsules was assessed using a mouse model of Citrobacter rodentium-induced colitis.
29958098	0	26	theme	Citrobacter	86:96	arg1	colitis					116:122	Citrobacter rodentium-induced colitis	86:122	Citrobacter rodentium-induced colitis in mice treated with probiotics	86:154	Pea-protein alginate encapsulation adversely affects development of clinical signs of Citrobacter rodentium-induced colitis in mice treated with probiotics.
29958098	1	27	theme	strains	177:183	arg1	efficacy					161:168	The efficacy	157:168	The efficacy of two strains of Lactobacillus probiotics (Lactobacillus rhamnosus R0011 and Lactobacillus helveticus R0052) immobilized in microcapsules composed of pea protein isolate (PPI) and alginate microcapsules	157:372	The efficacy of two strains of Lactobacillus probiotics (Lactobacillus rhamnosus R0011 and Lactobacillus helveticus R0052) immobilized in microcapsules composed of pea protein isolate (PPI) and alginate microcapsules was assessed using a mouse model of Citrobacter rodentium-induced colitis.
29958098	7	28	from	changes	1427:1433	arg1	most					1438:1441	most	1438:1441	most	1438:1441	Probiotics led to changes in most of the disease markers but did not have a significant impact on cytokine profiles in infected animals.
29958098	7	28	from	changes	1427:1433	arg1	markers					1458:1464	the disease markers	1446:1464	the disease markers	1446:1464	Probiotics led to changes in most of the disease markers but did not have a significant impact on cytokine profiles in infected animals.
29958098	2	29	theme	group	712:716	arg1	probiotics					700:709	no probiotics	697:709	no probiotics (group E)	697:719	Accordingly, 4-week-old mice were fed diets supplemented with freeze-dried probiotics (group P), probiotic-containing microcapsules (group PE) (lyophilized PPI-alginate microcapsules containing probiotics), or PPI-alginate microcapsules containing no probiotics (group E).
29958098	2	29	theme	group	712:716	arg1	E					718:718	group E	712:718	group E	712:718	Accordingly, 4-week-old mice were fed diets supplemented with freeze-dried probiotics (group P), probiotic-containing microcapsules (group PE) (lyophilized PPI-alginate microcapsules containing probiotics), or PPI-alginate microcapsules containing no probiotics (group E).
29958098	0	30	theme	colitis	116:122	arg1	signs					77:81	clinical signs	68:81	clinical signs of Citrobacter rodentium-induced colitis in mice treated with probiotics	68:154	Pea-protein alginate encapsulation adversely affects development of clinical signs of Citrobacter rodentium-induced colitis in mice treated with probiotics.
29958098	6	31	with	Infection	1191:1199	arg1	rodentium					1209:1217	C. rodentium	1206:1217	C. rodentium	1206:1217	Infection with C. rodentium led to marked progression of infectious colitis, as revealed by symptomatic and histopathological data, changes in cytokine expression, and alteration of composition of mucosal communities.
29958098	2	32	theme	probiotic-containing	546:565	arg1	microcapsules					567:579	probiotic-containing microcapsules	546:579	probiotic-containing microcapsules (group PE) (lyophilized PPI-alginate microcapsules containing probiotics)	546:653	Accordingly, 4-week-old mice were fed diets supplemented with freeze-dried probiotics (group P), probiotic-containing microcapsules (group PE) (lyophilized PPI-alginate microcapsules containing probiotics), or PPI-alginate microcapsules containing no probiotics (group E).
29958098	2	32	theme	probiotic-containing	546:565	arg1	PE					588:589	group PE	582:589	group PE	582:589	Accordingly, 4-week-old mice were fed diets supplemented with freeze-dried probiotics (group P), probiotic-containing microcapsules (group PE) (lyophilized PPI-alginate microcapsules containing probiotics), or PPI-alginate microcapsules containing no probiotics (group E).
29958098	6	33	theme	cytokine	1334:1341	arg1	expression					1343:1352	cytokine expression	1334:1352	cytokine expression	1334:1352	Infection with C. rodentium led to marked progression of infectious colitis, as revealed by symptomatic and histopathological data, changes in cytokine expression, and alteration of composition of mucosal communities.
29958098	1	34	theme	mouse	395:399	arg1	model					401:405	a mouse model	393:405	a mouse model of Citrobacter rodentium-induced colitis	393:446	The efficacy of two strains of Lactobacillus probiotics (Lactobacillus rhamnosus R0011 and Lactobacillus helveticus R0052) immobilized in microcapsules composed of pea protein isolate (PPI) and alginate microcapsules was assessed using a mouse model of Citrobacter rodentium-induced colitis.
29958098	5	35	theme	colonic	975:981	arg1	tissues					993:999	colonic and cecal tissues	975:999	colonic and cecal tissues	975:999	Histopathological changes in colonic and cecal tissues, cytokine expression levels, and pathogen and probiotic densities in feces were examined, and the microbial communities of the distal colon mucosa were characterized by 16S rRNA sequencing.
29958098	9	36	from	models	1965:1970	arg1	development					1899:1909	the development	1895:1909	the development of an inflammatory response in certain animal disease models	1895:1970	These results suggest that even though food ingredients may be generally recognized as safe, they may in fact contribute to the development of an inflammatory response in certain animal disease models.
29958098	5	37	theme	rRNA	1174:1177	arg1	sequencing					1179:1188	16S rRNA sequencing	1170:1188	16S rRNA sequencing	1170:1188	Histopathological changes in colonic and cecal tissues, cytokine expression levels, and pathogen and probiotic densities in feces were examined, and the microbial communities of the distal colon mucosa were characterized by 16S rRNA sequencing.
29958098	0	38	theme	Pea-protein	0:10	arg1	encapsulation					21:33	Pea-protein alginate encapsulation	0:33	Pea-protein alginate encapsulation	0:33	Pea-protein alginate encapsulation adversely affects development of clinical signs of Citrobacter rodentium-induced colitis in mice treated with probiotics.
29958098	2	39	theme	4-week-old	462:471	arg1	mice					473:476	4-week-old mice	462:476	4-week-old mice	462:476	Accordingly, 4-week-old mice were fed diets supplemented with freeze-dried probiotics (group P), probiotic-containing microcapsules (group PE) (lyophilized PPI-alginate microcapsules containing probiotics), or PPI-alginate microcapsules containing no probiotics (group E).
29958098	5	40	theme	16S	1170:1172	arg1	sequencing					1179:1188	16S rRNA sequencing	1170:1188	16S rRNA sequencing	1170:1188	Histopathological changes in colonic and cecal tissues, cytokine expression levels, and pathogen and probiotic densities in feces were examined, and the microbial communities of the distal colon mucosa were characterized by 16S rRNA sequencing.
29958098	1	41	dep	probiotics	202:211	arg1	probiotics					202:211	Lactobacillus probiotics	188:211	Lactobacillus probiotics (Lactobacillus rhamnosus R0011 and Lactobacillus helveticus R0052) immobilized in microcapsules composed of pea protein isolate (PPI) and alginate microcapsules	188:372	The efficacy of two strains of Lactobacillus probiotics (Lactobacillus rhamnosus R0011 and Lactobacillus helveticus R0052) immobilized in microcapsules composed of pea protein isolate (PPI) and alginate microcapsules was assessed using a mouse model of Citrobacter rodentium-induced colitis.
29958098	1	41	dep	probiotics	202:211	arg1	helveticus					262:271	Lactobacillus helveticus R0052	248:277	Lactobacillus helveticus R0052	248:277	The efficacy of two strains of Lactobacillus probiotics (Lactobacillus rhamnosus R0011 and Lactobacillus helveticus R0052) immobilized in microcapsules composed of pea protein isolate (PPI) and alginate microcapsules was assessed using a mouse model of Citrobacter rodentium-induced colitis.
29958098	1	41	dep	probiotics	202:211	arg1	Lactobacillus					214:226	Lactobacillus	214:226	Lactobacillus	214:226	The efficacy of two strains of Lactobacillus probiotics (Lactobacillus rhamnosus R0011 and Lactobacillus helveticus R0052) immobilized in microcapsules composed of pea protein isolate (PPI) and alginate microcapsules was assessed using a mouse model of Citrobacter rodentium-induced colitis.
29958098	2	42	contain	containing	632:641	arg2	probiotics					643:652	probiotics	643:652	probiotics	643:652	Accordingly, 4-week-old mice were fed diets supplemented with freeze-dried probiotics (group P), probiotic-containing microcapsules (group PE) (lyophilized PPI-alginate microcapsules containing probiotics), or PPI-alginate microcapsules containing no probiotics (group E).
29958098	2	42	contain	containing	632:641	arg1	microcapsules					618:630	lyophilized PPI-alginate microcapsules	593:630	lyophilized PPI-alginate microcapsules containing probiotics	593:652	Accordingly, 4-week-old mice were fed diets supplemented with freeze-dried probiotics (group P), probiotic-containing microcapsules (group PE) (lyophilized PPI-alginate microcapsules containing probiotics), or PPI-alginate microcapsules containing no probiotics (group E).
29958098	5	43	theme	distal	1128:1133	arg1	mucosa					1141:1146	the distal colon mucosa	1124:1146	the distal colon mucosa	1124:1146	Histopathological changes in colonic and cecal tissues, cytokine expression levels, and pathogen and probiotic densities in feces were examined, and the microbial communities of the distal colon mucosa were characterized by 16S rRNA sequencing.
29958098	2	44	dep	microcapsules	567:579	arg1	microcapsules					618:630	lyophilized PPI-alginate microcapsules	593:630	lyophilized PPI-alginate microcapsules containing probiotics	593:652	Accordingly, 4-week-old mice were fed diets supplemented with freeze-dried probiotics (group P), probiotic-containing microcapsules (group PE) (lyophilized PPI-alginate microcapsules containing probiotics), or PPI-alginate microcapsules containing no probiotics (group E).
29958098	5	45	theme	expression	1011:1020	arg1	levels					1022:1027	cytokine expression levels	1002:1027	cytokine expression levels	1002:1027	Histopathological changes in colonic and cecal tissues, cytokine expression levels, and pathogen and probiotic densities in feces were examined, and the microbial communities of the distal colon mucosa were characterized by 16S rRNA sequencing.
29958098	9	46	theme	food	1810:1813	arg1	safe					1858:1861	safe	1858:1861	safe	1858:1861	These results suggest that even though food ingredients may be generally recognized as safe, they may in fact contribute to the development of an inflammatory response in certain animal disease models.
29958098	9	46	theme	food	1810:1813	arg1	ingredients					1815:1825	food ingredients	1810:1825	food ingredients	1810:1825	These results suggest that even though food ingredients may be generally recognized as safe, they may in fact contribute to the development of an inflammatory response in certain animal disease models.
29958098	0	47	theme	signs	77:81	arg1	development					53:63	development	53:63	development of clinical signs of Citrobacter rodentium-induced colitis in mice treated with probiotics	53:154	Pea-protein alginate encapsulation adversely affects development of clinical signs of Citrobacter rodentium-induced colitis in mice treated with probiotics.
29958098	6	48	theme	mucosal	1388:1394	arg1	communities					1396:1406	mucosal communities	1388:1406	mucosal communities	1388:1406	Infection with C. rodentium led to marked progression of infectious colitis, as revealed by symptomatic and histopathological data, changes in cytokine expression, and alteration of composition of mucosal communities.
29958098	5	49	theme	mucosa	1141:1146	arg1	communities					1109:1119	the microbial communities	1095:1119	the microbial communities of the distal colon mucosa	1095:1146	Histopathological changes in colonic and cecal tissues, cytokine expression levels, and pathogen and probiotic densities in feces were examined, and the microbial communities of the distal colon mucosa were characterized by 16S rRNA sequencing.
29958098	1	50	theme	alginate	351:358	arg1	microcapsules					360:372	alginate microcapsules	351:372	alginate microcapsules	351:372	The efficacy of two strains of Lactobacillus probiotics (Lactobacillus rhamnosus R0011 and Lactobacillus helveticus R0052) immobilized in microcapsules composed of pea protein isolate (PPI) and alginate microcapsules was assessed using a mouse model of Citrobacter rodentium-induced colitis.
29958098	7	51	theme	significant	1485:1495	arg1	impact					1497:1502	a significant impact	1483:1502	a significant impact on cytokine profiles in infected animals	1483:1543	Probiotics led to changes in most of the disease markers but did not have a significant impact on cytokine profiles in infected animals.
29958098	1	52	theme	Lactobacillus	248:260	arg1	probiotics					202:211	Lactobacillus probiotics	188:211	Lactobacillus probiotics (Lactobacillus rhamnosus R0011 and Lactobacillus helveticus R0052) immobilized in microcapsules composed of pea protein isolate (PPI) and alginate microcapsules	188:372	The efficacy of two strains of Lactobacillus probiotics (Lactobacillus rhamnosus R0011 and Lactobacillus helveticus R0052) immobilized in microcapsules composed of pea protein isolate (PPI) and alginate microcapsules was assessed using a mouse model of Citrobacter rodentium-induced colitis.
29958098	1	52	theme	Lactobacillus	248:260	arg1	helveticus					262:271	Lactobacillus helveticus R0052	248:277	Lactobacillus helveticus R0052	248:277	The efficacy of two strains of Lactobacillus probiotics (Lactobacillus rhamnosus R0011 and Lactobacillus helveticus R0052) immobilized in microcapsules composed of pea protein isolate (PPI) and alginate microcapsules was assessed using a mouse model of Citrobacter rodentium-induced colitis.
29958098	5	53	theme	cecal	987:991	arg1	tissues					993:999	colonic and cecal tissues	975:999	colonic and cecal tissues	975:999	Histopathological changes in colonic and cecal tissues, cytokine expression levels, and pathogen and probiotic densities in feces were examined, and the microbial communities of the distal colon mucosa were characterized by 16S rRNA sequencing.
29958098	6	54	from	data	1317:1320	arg1	expression					1343:1352	cytokine expression	1334:1352	cytokine expression	1334:1352	Infection with C. rodentium led to marked progression of infectious colitis, as revealed by symptomatic and histopathological data, changes in cytokine expression, and alteration of composition of mucosal communities.
29958098	1	55	dep	Lactobacillus	214:226	arg1	rhamnosus					228:236	Lactobacillus rhamnosus R0011	214:242	Lactobacillus rhamnosus R0011	214:242	The efficacy of two strains of Lactobacillus probiotics (Lactobacillus rhamnosus R0011 and Lactobacillus helveticus R0052) immobilized in microcapsules composed of pea protein isolate (PPI) and alginate microcapsules was assessed using a mouse model of Citrobacter rodentium-induced colitis.
29958098	1	55	dep	Lactobacillus	214:226	arg1	R0011					238:242	R0011	238:242	Lactobacillus rhamnosus R0011	214:242	The efficacy of two strains of Lactobacillus probiotics (Lactobacillus rhamnosus R0011 and Lactobacillus helveticus R0052) immobilized in microcapsules composed of pea protein isolate (PPI) and alginate microcapsules was assessed using a mouse model of Citrobacter rodentium-induced colitis.
29958098	7	56	contain	have	1478:1481	arg1	Probiotics					1409:1418	Probiotics	1409:1418	Probiotics	1409:1418	Probiotics led to changes in most of the disease markers but did not have a significant impact on cytokine profiles in infected animals.
29958098	7	56	contain	have	1478:1481	arg2	impact					1497:1502	a significant impact	1483:1502	a significant impact on cytokine profiles in infected animals	1483:1543	Probiotics led to changes in most of the disease markers but did not have a significant impact on cytokine profiles in infected animals.
29958098	0	57	theme	clinical	68:75	arg1	signs					77:81	clinical signs	68:81	clinical signs of Citrobacter rodentium-induced colitis in mice treated with probiotics	68:154	Pea-protein alginate encapsulation adversely affects development of clinical signs of Citrobacter rodentium-induced colitis in mice treated with probiotics.
29958098	4	58	dep	symptoms	868:875	arg1	diarrhea					897:904	diarrhea	897:904	diarrhea	897:904	Daily monitoring of disease symptoms (abnormal behavior, diarrhea, etc.) and body weights was undertaken.
29958098	4	58	dep	symptoms	868:875	arg1	behavior					887:894	abnormal behavior	878:894	abnormal behavior	878:894	Daily monitoring of disease symptoms (abnormal behavior, diarrhea, etc.) and body weights was undertaken.
29958098	4	58	dep	symptoms	868:875	arg1	etc.					907:910	etc.	907:910	etc.	907:910	Daily monitoring of disease symptoms (abnormal behavior, diarrhea, etc.) and body weights was undertaken.
29958098	5	59	theme	colon	1135:1139	arg1	mucosa					1141:1146	the distal colon mucosa	1124:1146	the distal colon mucosa	1124:1146	Histopathological changes in colonic and cecal tissues, cytokine expression levels, and pathogen and probiotic densities in feces were examined, and the microbial communities of the distal colon mucosa were characterized by 16S rRNA sequencing.
29958098	7	60	from	impact	1497:1502	arg1	profiles					1516:1523	cytokine profiles	1507:1523	cytokine profiles in infected animals	1507:1543	Probiotics led to changes in most of the disease markers but did not have a significant impact on cytokine profiles in infected animals.
29958098	4	61	theme	disease	860:866	arg1	symptoms					868:875	disease symptoms	860:875	disease symptoms (abnormal behavior, diarrhea, etc.)	860:911	Daily monitoring of disease symptoms (abnormal behavior, diarrhea, etc.) and body weights was undertaken.
29958098	8	62	theme	data	1613:1616	arg1	evident					1626:1632	evident	1626:1632	evident	1626:1632	On the basis of cytokine expression analyses and histopathological data, it was evident that encapsulation materials (pea protein and calcium alginate) contributed to inflammation and worsened a set of symptoms in the cecum.
29958098	3	63	theme	mice	734:737	arg1	Half					722:725	Half	722:725	Half of the mice (controls, groups P, PE, and E)	722:769	Half of the mice (controls, groups P, PE, and E) received C. rodentium by gavage 2 weeks after initiation of feeding.
29958098	5	64	from	changes	964:970	arg1	tissues					993:999	colonic and cecal tissues	975:999	colonic and cecal tissues	975:999	Histopathological changes in colonic and cecal tissues, cytokine expression levels, and pathogen and probiotic densities in feces were examined, and the microbial communities of the distal colon mucosa were characterized by 16S rRNA sequencing.
29958098	5	64	from	changes	964:970	arg1	feces					1070:1074	feces	1070:1074	feces	1070:1074	Histopathological changes in colonic and cecal tissues, cytokine expression levels, and pathogen and probiotic densities in feces were examined, and the microbial communities of the distal colon mucosa were characterized by 16S rRNA sequencing.
29958098	6	65	theme	infectious	1248:1257	arg1	colitis					1259:1265	infectious colitis	1248:1265	infectious colitis	1248:1265	Infection with C. rodentium led to marked progression of infectious colitis, as revealed by symptomatic and histopathological data, changes in cytokine expression, and alteration of composition of mucosal communities.
29958098	8	66	theme	calcium	1680:1686	arg1	materials					1653:1661	encapsulation materials	1639:1661	encapsulation materials (pea protein and calcium alginate)	1639:1696	On the basis of cytokine expression analyses and histopathological data, it was evident that encapsulation materials (pea protein and calcium alginate) contributed to inflammation and worsened a set of symptoms in the cecum.
29958098	8	66	theme	calcium	1680:1686	arg1	alginate					1688:1695	calcium alginate	1680:1695	calcium alginate	1680:1695	On the basis of cytokine expression analyses and histopathological data, it was evident that encapsulation materials (pea protein and calcium alginate) contributed to inflammation and worsened a set of symptoms in the cecum.
29958098	1	67	theme	probiotics	202:211	arg1	strains					177:183	two strains	173:183	two strains of Lactobacillus probiotics (Lactobacillus rhamnosus R0011 and Lactobacillus helveticus R0052) immobilized in microcapsules composed of pea protein isolate (PPI) and alginate microcapsules	173:372	The efficacy of two strains of Lactobacillus probiotics (Lactobacillus rhamnosus R0011 and Lactobacillus helveticus R0052) immobilized in microcapsules composed of pea protein isolate (PPI) and alginate microcapsules was assessed using a mouse model of Citrobacter rodentium-induced colitis.
29958098	2	68	theme	PPI-alginate	659:670	arg1	microcapsules					672:684	PPI-alginate microcapsules	659:684	PPI-alginate microcapsules containing no probiotics (group E)	659:719	Accordingly, 4-week-old mice were fed diets supplemented with freeze-dried probiotics (group P), probiotic-containing microcapsules (group PE) (lyophilized PPI-alginate microcapsules containing probiotics), or PPI-alginate microcapsules containing no probiotics (group E).
29958098	1	69	theme	Citrobacter	410:420	arg1	colitis					440:446	Citrobacter rodentium-induced colitis	410:446	Citrobacter rodentium-induced colitis	410:446	The efficacy of two strains of Lactobacillus probiotics (Lactobacillus rhamnosus R0011 and Lactobacillus helveticus R0052) immobilized in microcapsules composed of pea protein isolate (PPI) and alginate microcapsules was assessed using a mouse model of Citrobacter rodentium-induced colitis.
29958098	2	70	theme	group	582:586	arg1	microcapsules					567:579	probiotic-containing microcapsules	546:579	probiotic-containing microcapsules (group PE) (lyophilized PPI-alginate microcapsules containing probiotics)	546:653	Accordingly, 4-week-old mice were fed diets supplemented with freeze-dried probiotics (group P), probiotic-containing microcapsules (group PE) (lyophilized PPI-alginate microcapsules containing probiotics), or PPI-alginate microcapsules containing no probiotics (group E).
29958098	2	70	theme	group	582:586	arg1	PE					588:589	group PE	582:589	group PE	582:589	Accordingly, 4-week-old mice were fed diets supplemented with freeze-dried probiotics (group P), probiotic-containing microcapsules (group PE) (lyophilized PPI-alginate microcapsules containing probiotics), or PPI-alginate microcapsules containing no probiotics (group E).
29958098	6	71	theme	histopathological	1299:1315	arg1	data					1317:1320	symptomatic and histopathological data	1283:1320	symptomatic and histopathological data	1283:1320	Infection with C. rodentium led to marked progression of infectious colitis, as revealed by symptomatic and histopathological data, changes in cytokine expression, and alteration of composition of mucosal communities.
29958098	3	72	dep	weeks	805:809	arg1	initiation					817:826	initiation	817:826	initiation of feeding	817:837	Half of the mice (controls, groups P, PE, and E) received C. rodentium by gavage 2 weeks after initiation of feeding.
29958098	6	73	theme	C.	1206:1207	arg1	rodentium					1209:1217	C. rodentium	1206:1217	C. rodentium	1206:1217	Infection with C. rodentium led to marked progression of infectious colitis, as revealed by symptomatic and histopathological data, changes in cytokine expression, and alteration of composition of mucosal communities.
29958098	9	74	theme	response	1930:1937	arg1	development					1899:1909	the development	1895:1909	the development of an inflammatory response in certain animal disease models	1895:1970	These results suggest that even though food ingredients may be generally recognized as safe, they may in fact contribute to the development of an inflammatory response in certain animal disease models.
29958098	6	75	theme	symptomatic	1283:1293	arg1	data					1317:1320	symptomatic and histopathological data	1283:1320	symptomatic and histopathological data	1283:1320	Infection with C. rodentium led to marked progression of infectious colitis, as revealed by symptomatic and histopathological data, changes in cytokine expression, and alteration of composition of mucosal communities.
29958098	5	76	theme	pathogen	1034:1041	arg1	densities					1057:1065	pathogen and probiotic densities	1034:1065	densities	1057:1065	Histopathological changes in colonic and cecal tissues, cytokine expression levels, and pathogen and probiotic densities in feces were examined, and the microbial communities of the distal colon mucosa were characterized by 16S rRNA sequencing.
29958098	2	77	theme	lyophilized	593:603	arg1	microcapsules					618:630	lyophilized PPI-alginate microcapsules	593:630	lyophilized PPI-alginate microcapsules containing probiotics	593:652	Accordingly, 4-week-old mice were fed diets supplemented with freeze-dried probiotics (group P), probiotic-containing microcapsules (group PE) (lyophilized PPI-alginate microcapsules containing probiotics), or PPI-alginate microcapsules containing no probiotics (group E).
29958098	8	78	dep	materials	1653:1661	arg1	materials					1653:1661	encapsulation materials	1639:1661	encapsulation materials (pea protein and calcium alginate)	1639:1696	On the basis of cytokine expression analyses and histopathological data, it was evident that encapsulation materials (pea protein and calcium alginate) contributed to inflammation and worsened a set of symptoms in the cecum.
29958098	8	78	dep	materials	1653:1661	arg1	protein					1668:1674	pea protein	1664:1674	pea protein	1664:1674	On the basis of cytokine expression analyses and histopathological data, it was evident that encapsulation materials (pea protein and calcium alginate) contributed to inflammation and worsened a set of symptoms in the cecum.
29958098	8	78	dep	materials	1653:1661	arg1	alginate					1688:1695	calcium alginate	1680:1695	calcium alginate	1680:1695	On the basis of cytokine expression analyses and histopathological data, it was evident that encapsulation materials (pea protein and calcium alginate) contributed to inflammation and worsened a set of symptoms in the cecum.
29958098	5	79	theme	probiotic	1047:1055	arg1	densities					1057:1065	pathogen and probiotic densities	1034:1065	densities	1057:1065	Histopathological changes in colonic and cecal tissues, cytokine expression levels, and pathogen and probiotic densities in feces were examined, and the microbial communities of the distal colon mucosa were characterized by 16S rRNA sequencing.
29958098	9	80	theme	disease	1957:1963	arg1	models					1965:1970	certain animal disease models	1942:1970	certain animal disease models	1942:1970	These results suggest that even though food ingredients may be generally recognized as safe, they may in fact contribute to the development of an inflammatory response in certain animal disease models.
29958098	0	81	theme	rodentium-induced	98:114	arg1	colitis					116:122	Citrobacter rodentium-induced colitis	86:122	Citrobacter rodentium-induced colitis in mice treated with probiotics	86:154	Pea-protein alginate encapsulation adversely affects development of clinical signs of Citrobacter rodentium-induced colitis in mice treated with probiotics.
29958098	6	82	from	alteration	1359:1368	arg1	expression					1343:1352	cytokine expression	1334:1352	cytokine expression	1334:1352	Infection with C. rodentium led to marked progression of infectious colitis, as revealed by symptomatic and histopathological data, changes in cytokine expression, and alteration of composition of mucosal communities.
29958098	2	83	contain	containing	686:695	arg1	microcapsules					672:684	PPI-alginate microcapsules	659:684	PPI-alginate microcapsules containing no probiotics (group E)	659:719	Accordingly, 4-week-old mice were fed diets supplemented with freeze-dried probiotics (group P), probiotic-containing microcapsules (group PE) (lyophilized PPI-alginate microcapsules containing probiotics), or PPI-alginate microcapsules containing no probiotics (group E).
29958098	2	83	contain	containing	686:695	arg2	E					718:718	group E	712:718	group E	712:718	Accordingly, 4-week-old mice were fed diets supplemented with freeze-dried probiotics (group P), probiotic-containing microcapsules (group PE) (lyophilized PPI-alginate microcapsules containing probiotics), or PPI-alginate microcapsules containing no probiotics (group E).
29958098	2	83	contain	containing	686:695	arg2	probiotics					700:709	no probiotics	697:709	no probiotics (group E)	697:719	Accordingly, 4-week-old mice were fed diets supplemented with freeze-dried probiotics (group P), probiotic-containing microcapsules (group PE) (lyophilized PPI-alginate microcapsules containing probiotics), or PPI-alginate microcapsules containing no probiotics (group E).
29958098	3	84	theme	feeding	831:837	arg1	initiation					817:826	initiation	817:826	initiation of feeding	817:837	Half of the mice (controls, groups P, PE, and E) received C. rodentium by gavage 2 weeks after initiation of feeding.
29958098	5	85	theme	Histopathological	946:962	arg1	changes					964:970	Histopathological changes	946:970	Histopathological changes in colonic and cecal tissues	946:999	Histopathological changes in colonic and cecal tissues, cytokine expression levels, and pathogen and probiotic densities in feces were examined, and the microbial communities of the distal colon mucosa were characterized by 16S rRNA sequencing.
29958098	7	86	theme	infected	1528:1535	arg1	animals					1537:1543	infected animals	1528:1543	infected animals	1528:1543	Probiotics led to changes in most of the disease markers but did not have a significant impact on cytokine profiles in infected animals.
29958098	4	87	theme	weights	922:928	arg1	monitoring					846:855	Daily monitoring	840:855	Daily monitoring of disease symptoms (abnormal behavior, diarrhea, etc.) and body weights	840:928	Daily monitoring of disease symptoms (abnormal behavior, diarrhea, etc.) and body weights was undertaken.
29958098	5	88	from	densities	1057:1065	arg1	tissues					993:999	colonic and cecal tissues	975:999	colonic and cecal tissues	975:999	Histopathological changes in colonic and cecal tissues, cytokine expression levels, and pathogen and probiotic densities in feces were examined, and the microbial communities of the distal colon mucosa were characterized by 16S rRNA sequencing.
29958098	5	88	from	densities	1057:1065	arg1	feces					1070:1074	feces	1070:1074	feces	1070:1074	Histopathological changes in colonic and cecal tissues, cytokine expression levels, and pathogen and probiotic densities in feces were examined, and the microbial communities of the distal colon mucosa were characterized by 16S rRNA sequencing.
29958098	8	89	theme	expression	1571:1580	arg1	analyses					1582:1589	cytokine expression analyses	1562:1589	cytokine expression analyses	1562:1589	On the basis of cytokine expression analyses and histopathological data, it was evident that encapsulation materials (pea protein and calcium alginate) contributed to inflammation and worsened a set of symptoms in the cecum.
29958098	0	90	theme	alginate	12:19	arg1	encapsulation					21:33	Pea-protein alginate encapsulation	0:33	Pea-protein alginate encapsulation	0:33	Pea-protein alginate encapsulation adversely affects development of clinical signs of Citrobacter rodentium-induced colitis in mice treated with probiotics.
29958098	2	91	theme	fed	483:485	arg1	diets					487:491	fed diets	483:491	fed diets supplemented with freeze-dried probiotics (group P), probiotic-containing microcapsules (group PE) (lyophilized PPI-alginate microcapsules containing probiotics), or PPI-alginate microcapsules containing no probiotics (group E)	483:719	Accordingly, 4-week-old mice were fed diets supplemented with freeze-dried probiotics (group P), probiotic-containing microcapsules (group PE) (lyophilized PPI-alginate microcapsules containing probiotics), or PPI-alginate microcapsules containing no probiotics (group E).
29958098	1	92	theme	pea	321:323	arg1	PPI					342:344	PPI	342:344	PPI	342:344	The efficacy of two strains of Lactobacillus probiotics (Lactobacillus rhamnosus R0011 and Lactobacillus helveticus R0052) immobilized in microcapsules composed of pea protein isolate (PPI) and alginate microcapsules was assessed using a mouse model of Citrobacter rodentium-induced colitis.
29958098	1	92	theme	pea	321:323	arg1	isolate					333:339	pea protein isolate	321:339	pea protein isolate (PPI)	321:345	The efficacy of two strains of Lactobacillus probiotics (Lactobacillus rhamnosus R0011 and Lactobacillus helveticus R0052) immobilized in microcapsules composed of pea protein isolate (PPI) and alginate microcapsules was assessed using a mouse model of Citrobacter rodentium-induced colitis.
29958098	9	93	from	response	1930:1937	arg1	models					1965:1970	certain animal disease models	1942:1970	certain animal disease models	1942:1970	These results suggest that even though food ingredients may be generally recognized as safe, they may in fact contribute to the development of an inflammatory response in certain animal disease models.
29958098	6	94	from	changes	1323:1329	arg1	expression					1343:1352	cytokine expression	1334:1352	cytokine expression	1334:1352	Infection with C. rodentium led to marked progression of infectious colitis, as revealed by symptomatic and histopathological data, changes in cytokine expression, and alteration of composition of mucosal communities.
29958098	5	95	theme	cytokine	1002:1009	arg1	levels					1022:1027	cytokine expression levels	1002:1027	cytokine expression levels	1002:1027	Histopathological changes in colonic and cecal tissues, cytokine expression levels, and pathogen and probiotic densities in feces were examined, and the microbial communities of the distal colon mucosa were characterized by 16S rRNA sequencing.
29958098	8	96	theme	encapsulation	1639:1651	arg1	materials					1653:1661	encapsulation materials	1639:1661	encapsulation materials (pea protein and calcium alginate)	1639:1696	On the basis of cytokine expression analyses and histopathological data, it was evident that encapsulation materials (pea protein and calcium alginate) contributed to inflammation and worsened a set of symptoms in the cecum.
29958098	8	96	theme	encapsulation	1639:1651	arg1	protein					1668:1674	pea protein	1664:1674	pea protein	1664:1674	On the basis of cytokine expression analyses and histopathological data, it was evident that encapsulation materials (pea protein and calcium alginate) contributed to inflammation and worsened a set of symptoms in the cecum.
29958098	8	96	theme	encapsulation	1639:1651	arg1	alginate					1688:1695	calcium alginate	1680:1695	calcium alginate	1680:1695	On the basis of cytokine expression analyses and histopathological data, it was evident that encapsulation materials (pea protein and calcium alginate) contributed to inflammation and worsened a set of symptoms in the cecum.
29958098	2	97	theme	freeze-dried	511:522	arg1	P					542:542	group P	536:542	group P	536:542	Accordingly, 4-week-old mice were fed diets supplemented with freeze-dried probiotics (group P), probiotic-containing microcapsules (group PE) (lyophilized PPI-alginate microcapsules containing probiotics), or PPI-alginate microcapsules containing no probiotics (group E).
29958098	2	97	theme	freeze-dried	511:522	arg1	probiotics					524:533	freeze-dried probiotics	511:533	freeze-dried probiotics (group P)	511:543	Accordingly, 4-week-old mice were fed diets supplemented with freeze-dried probiotics (group P), probiotic-containing microcapsules (group PE) (lyophilized PPI-alginate microcapsules containing probiotics), or PPI-alginate microcapsules containing no probiotics (group E).
29958098	6	98	theme	communities	1396:1406	arg1	composition					1373:1383	composition	1373:1383	composition of mucosal communities	1373:1406	Infection with C. rodentium led to marked progression of infectious colitis, as revealed by symptomatic and histopathological data, changes in cytokine expression, and alteration of composition of mucosal communities.
29958098	3	99	dep	groups	750:755	arg1	groups					750:755	groups P, PE, and E	750:768	groups P, PE, and E	750:768	Half of the mice (controls, groups P, PE, and E) received C. rodentium by gavage 2 weeks after initiation of feeding.
29958098	3	99	dep	groups	750:755	arg1	PE					760:761	PE	760:761	PE	760:761	Half of the mice (controls, groups P, PE, and E) received C. rodentium by gavage 2 weeks after initiation of feeding.
29958098	3	99	dep	groups	750:755	arg1	E					768:768	E	768:768	E	768:768	Half of the mice (controls, groups P, PE, and E) received C. rodentium by gavage 2 weeks after initiation of feeding.
29958098	3	99	dep	groups	750:755	arg1	P					757:757	P	757:757	P	757:757	Half of the mice (controls, groups P, PE, and E) received C. rodentium by gavage 2 weeks after initiation of feeding.
29958098	7	100	theme	cytokine	1507:1514	arg1	profiles					1516:1523	cytokine profiles	1507:1523	cytokine profiles in infected animals	1507:1543	Probiotics led to changes in most of the disease markers but did not have a significant impact on cytokine profiles in infected animals.
29958098	8	101	dep	analyses	1582:1589	arg1	basis					1553:1557	basis	1553:1557	basis	1553:1557	On the basis of cytokine expression analyses and histopathological data, it was evident that encapsulation materials (pea protein and calcium alginate) contributed to inflammation and worsened a set of symptoms in the cecum.
29958098	8	101	dep	analyses	1582:1589	arg1	the					1549:1551	the	1549:1551	the	1549:1551	On the basis of cytokine expression analyses and histopathological data, it was evident that encapsulation materials (pea protein and calcium alginate) contributed to inflammation and worsened a set of symptoms in the cecum.
29958098	8	102	theme	cytokine	1562:1569	arg1	analyses					1582:1589	cytokine expression analyses	1562:1589	cytokine expression analyses	1562:1589	On the basis of cytokine expression analyses and histopathological data, it was evident that encapsulation materials (pea protein and calcium alginate) contributed to inflammation and worsened a set of symptoms in the cecum.
29958098	3	103	dep	controls	740:747	arg1	groups					750:755	groups P, PE, and E	750:768	groups P, PE, and E	750:768	Half of the mice (controls, groups P, PE, and E) received C. rodentium by gavage 2 weeks after initiation of feeding.
29958098	3	103	dep	controls	740:747	arg1	PE					760:761	PE	760:761	PE	760:761	Half of the mice (controls, groups P, PE, and E) received C. rodentium by gavage 2 weeks after initiation of feeding.
29958098	3	103	dep	controls	740:747	arg1	E					768:768	E	768:768	E	768:768	Half of the mice (controls, groups P, PE, and E) received C. rodentium by gavage 2 weeks after initiation of feeding.
29958098	3	103	dep	controls	740:747	arg1	P					757:757	P	757:757	P	757:757	Half of the mice (controls, groups P, PE, and E) received C. rodentium by gavage 2 weeks after initiation of feeding.
29958098	8	104	theme	analyses	1582:1589	arg1	evident					1626:1632	evident	1626:1632	evident	1626:1632	On the basis of cytokine expression analyses and histopathological data, it was evident that encapsulation materials (pea protein and calcium alginate) contributed to inflammation and worsened a set of symptoms in the cecum.
29958098	8	105	theme	histopathological	1595:1611	arg1	data					1613:1616	histopathological data	1595:1616	histopathological data	1595:1616	On the basis of cytokine expression analyses and histopathological data, it was evident that encapsulation materials (pea protein and calcium alginate) contributed to inflammation and worsened a set of symptoms in the cecum.
29958098	8	106	theme	pea	1664:1666	arg1	materials					1653:1661	encapsulation materials	1639:1661	encapsulation materials (pea protein and calcium alginate)	1639:1696	On the basis of cytokine expression analyses and histopathological data, it was evident that encapsulation materials (pea protein and calcium alginate) contributed to inflammation and worsened a set of symptoms in the cecum.
29958098	8	106	theme	pea	1664:1666	arg1	protein					1668:1674	pea protein	1664:1674	pea protein	1664:1674	On the basis of cytokine expression analyses and histopathological data, it was evident that encapsulation materials (pea protein and calcium alginate) contributed to inflammation and worsened a set of symptoms in the cecum.
29773393	5	0	theme	inhibitory	1028:1037	arg1	effects					1039:1045	the strongest inhibitory effects	1014:1045	the strongest inhibitory effects on α-glycosidase and α-amylase activities	1014:1087	Moreover, HEP-C exhibited the strongest inhibitory effects on α-glycosidase and α-amylase activities.
29773393	3	1	theme	neutral	719:725	arg1	sugar					727:731	neutral sugar	719:731	neutral sugar	719:731	Results showed that the extraction solvents exhibited significant effects on the extraction yields, molecular weights, monosaccharide compositions, preliminary structural characteristics, microstructures of HEPs and on their contents, such as neutral sugar, uronic acid, protein, and β-(1 → 3)-glucan.
29773393	1	2	dep	polysaccharides	284:298	arg1	polysaccharides					284:298	water-soluble H. erinaceus polysaccharides	257:298	water-soluble H. erinaceus polysaccharides (HEPs; HEP-W, HEP-S, HEP-C, and HEP-A) from the fruit body of Hericium erinaceus	257:379	In this study, hot water, 0.9% NaCl, citric acid, and 1.25 M NaOH/0.05% NaBH4 were separately used for the extraction of water-soluble H. erinaceus polysaccharides (HEPs; HEP-W, HEP-S, HEP-C, and HEP-A) from the fruit body of Hericium erinaceus.
29773393	1	2	dep	polysaccharides	284:298	arg1	HEPs					301:304	HEPs	301:304	HEPs	301:304	In this study, hot water, 0.9% NaCl, citric acid, and 1.25 M NaOH/0.05% NaBH4 were separately used for the extraction of water-soluble H. erinaceus polysaccharides (HEPs; HEP-W, HEP-S, HEP-C, and HEP-A) from the fruit body of Hericium erinaceus.
29773393	1	2	dep	polysaccharides	284:298	arg1	HEP-C					321:325	HEP-C	321:325	HEP-C	321:325	In this study, hot water, 0.9% NaCl, citric acid, and 1.25 M NaOH/0.05% NaBH4 were separately used for the extraction of water-soluble H. erinaceus polysaccharides (HEPs; HEP-W, HEP-S, HEP-C, and HEP-A) from the fruit body of Hericium erinaceus.
29773393	1	2	dep	polysaccharides	284:298	arg1	HEP-W					307:311	HEP-W	307:311	HEP-W	307:311	In this study, hot water, 0.9% NaCl, citric acid, and 1.25 M NaOH/0.05% NaBH4 were separately used for the extraction of water-soluble H. erinaceus polysaccharides (HEPs; HEP-W, HEP-S, HEP-C, and HEP-A) from the fruit body of Hericium erinaceus.
29773393	1	2	dep	polysaccharides	284:298	arg1	HEP-A					332:336	HEP-A	332:336	HEP-A	332:336	In this study, hot water, 0.9% NaCl, citric acid, and 1.25 M NaOH/0.05% NaBH4 were separately used for the extraction of water-soluble H. erinaceus polysaccharides (HEPs; HEP-W, HEP-S, HEP-C, and HEP-A) from the fruit body of Hericium erinaceus.
29773393	1	2	dep	polysaccharides	284:298	arg1	HEP-S					314:318	HEP-S	314:318	HEP-S	314:318	In this study, hot water, 0.9% NaCl, citric acid, and 1.25 M NaOH/0.05% NaBH4 were separately used for the extraction of water-soluble H. erinaceus polysaccharides (HEPs; HEP-W, HEP-S, HEP-C, and HEP-A) from the fruit body of Hericium erinaceus.
29773393	0	3	theme	polysaccharides	86:100	arg1	bioactivity					52:62	bioactivity	52:62	bioactivity of Hericium erinaceus polysaccharides at different solvent extractions	52:133	Comparative study of physicochemical properties and bioactivity of Hericium erinaceus polysaccharides at different solvent extractions.
29773393	0	3	theme	polysaccharides	86:100	arg1	study					12:16	Comparative study	0:16	Comparative study of physicochemical properties	0:46	Comparative study of physicochemical properties and bioactivity of Hericium erinaceus polysaccharides at different solvent extractions.
29773393	3	4	theme	HEPs	683:686	arg1	yields					568:573	the extraction yields	553:573	the extraction yields	553:573	Results showed that the extraction solvents exhibited significant effects on the extraction yields, molecular weights, monosaccharide compositions, preliminary structural characteristics, microstructures of HEPs and on their contents, such as neutral sugar, uronic acid, protein, and β-(1 → 3)-glucan.
29773393	3	4	theme	HEPs	683:686	arg1	weights					586:592	molecular weights	576:592	molecular weights	576:592	Results showed that the extraction solvents exhibited significant effects on the extraction yields, molecular weights, monosaccharide compositions, preliminary structural characteristics, microstructures of HEPs and on their contents, such as neutral sugar, uronic acid, protein, and β-(1 → 3)-glucan.
29773393	3	4	theme	HEPs	683:686	arg1	compositions					610:621	monosaccharide compositions	595:621	monosaccharide compositions	595:621	Results showed that the extraction solvents exhibited significant effects on the extraction yields, molecular weights, monosaccharide compositions, preliminary structural characteristics, microstructures of HEPs and on their contents, such as neutral sugar, uronic acid, protein, and β-(1 → 3)-glucan.
29773393	3	4	theme	HEPs	683:686	arg1	microstructures					664:678	microstructures	664:678	microstructures	664:678	Results showed that the extraction solvents exhibited significant effects on the extraction yields, molecular weights, monosaccharide compositions, preliminary structural characteristics, microstructures of HEPs and on their contents, such as neutral sugar, uronic acid, protein, and β-(1 → 3)-glucan.
29773393	3	4	theme	HEPs	683:686	arg1	characteristics					647:661	preliminary structural characteristics	624:661	preliminary structural characteristics	624:661	Results showed that the extraction solvents exhibited significant effects on the extraction yields, molecular weights, monosaccharide compositions, preliminary structural characteristics, microstructures of HEPs and on their contents, such as neutral sugar, uronic acid, protein, and β-(1 → 3)-glucan.
29773393	1	5	theme	erinaceus	371:379	arg1	body					354:357	the fruit body	344:357	the fruit body of Hericium erinaceus	344:379	In this study, hot water, 0.9% NaCl, citric acid, and 1.25 M NaOH/0.05% NaBH4 were separately used for the extraction of water-soluble H. erinaceus polysaccharides (HEPs; HEP-W, HEP-S, HEP-C, and HEP-A) from the fruit body of Hericium erinaceus.
29773393	1	6	theme	0.9	162:164	arg1	%					165:165	%	165:165	%	165:165	In this study, hot water, 0.9% NaCl, citric acid, and 1.25 M NaOH/0.05% NaBH4 were separately used for the extraction of water-soluble H. erinaceus polysaccharides (HEPs; HEP-W, HEP-S, HEP-C, and HEP-A) from the fruit body of Hericium erinaceus.
29773393	6	7	from	applications	1191:1202	arg1	cosmetics					1227:1235	cosmetics	1227:1235	cosmetics	1227:1235	Therefore, HEP-C extracted with citric acid can be developed as a potential bioactive ingredient for applications in food, medicine, and cosmetics industries.
29773393	6	7	from	applications	1191:1202	arg1	food					1207:1210	food	1207:1210	food	1207:1210	Therefore, HEP-C extracted with citric acid can be developed as a potential bioactive ingredient for applications in food, medicine, and cosmetics industries.
29773393	6	7	from	applications	1191:1202	arg1	medicine					1213:1220	medicine	1213:1220	medicine	1213:1220	Therefore, HEP-C extracted with citric acid can be developed as a potential bioactive ingredient for applications in food, medicine, and cosmetics industries.
29773393	0	8	theme	solvent	115:121	arg1	extractions					123:133	different solvent extractions	105:133	different solvent extractions	105:133	Comparative study of physicochemical properties and bioactivity of Hericium erinaceus polysaccharides at different solvent extractions.
29773393	3	9	theme	extraction	500:509	arg1	solvents					511:518	the extraction solvents	496:518	the extraction solvents	496:518	Results showed that the extraction solvents exhibited significant effects on the extraction yields, molecular weights, monosaccharide compositions, preliminary structural characteristics, microstructures of HEPs and on their contents, such as neutral sugar, uronic acid, protein, and β-(1 → 3)-glucan.
29773393	6	10	theme	citric	1122:1127	arg1	acid					1129:1132	citric acid	1122:1132	citric acid	1122:1132	Therefore, HEP-C extracted with citric acid can be developed as a potential bioactive ingredient for applications in food, medicine, and cosmetics industries.
29773393	4	11	theme	antioxidant	943:953	arg1	capacities					955:964	antioxidant capacities	943:964	antioxidant capacities	943:964	In vitro antioxidant activity assays indicated that HEP-C extracted with citric acid solution showed stronger scavenging abilities on hydroxyl and DPPH radicals and antioxidant capacities than HEP-W and HEP-S.
29773393	0	12	theme	different	105:113	arg1	extractions					123:133	different solvent extractions	105:133	different solvent extractions	105:133	Comparative study of physicochemical properties and bioactivity of Hericium erinaceus polysaccharides at different solvent extractions.
29773393	5	13	theme	α-amylase	1068:1076	arg1	activities					1078:1087	α-glycosidase and α-amylase activities	1050:1087	α-glycosidase and α-amylase activities	1050:1087	Moreover, HEP-C exhibited the strongest inhibitory effects on α-glycosidase and α-amylase activities.
29773393	4	14	theme	DPPH	925:928	arg1	radicals					930:937	hydroxyl and DPPH radicals	912:937	radicals	930:937	In vitro antioxidant activity assays indicated that HEP-C extracted with citric acid solution showed stronger scavenging abilities on hydroxyl and DPPH radicals and antioxidant capacities than HEP-W and HEP-S.
29773393	4	15	theme	antioxidant	787:797	arg1	assays					808:813	In vitro antioxidant activity assays	778:813	In vitro antioxidant activity assays	778:813	In vitro antioxidant activity assays indicated that HEP-C extracted with citric acid solution showed stronger scavenging abilities on hydroxyl and DPPH radicals and antioxidant capacities than HEP-W and HEP-S.
29773393	1	16	theme	%	165:165	arg1	NaCl					167:170	0.9% NaCl	162:170	0.9% NaCl	162:170	In this study, hot water, 0.9% NaCl, citric acid, and 1.25 M NaOH/0.05% NaBH4 were separately used for the extraction of water-soluble H. erinaceus polysaccharides (HEPs; HEP-W, HEP-S, HEP-C, and HEP-A) from the fruit body of Hericium erinaceus.
29773393	3	17	theme	1 → 3	763:767	arg1	-glucan					769:775	β-(1 → 3)-glucan	760:775	β-(1 → 3)-glucan	760:775	Results showed that the extraction solvents exhibited significant effects on the extraction yields, molecular weights, monosaccharide compositions, preliminary structural characteristics, microstructures of HEPs and on their contents, such as neutral sugar, uronic acid, protein, and β-(1 → 3)-glucan.
29773393	5	18	from	effects	1039:1045	arg1	activities					1078:1087	α-glycosidase and α-amylase activities	1050:1087	α-glycosidase and α-amylase activities	1050:1087	Moreover, HEP-C exhibited the strongest inhibitory effects on α-glycosidase and α-amylase activities.
29773393	2	19	theme	physicochemical	386:400	arg1	properties					402:411	The physicochemical properties	382:411	The physicochemical properties	382:411	The physicochemical properties and biological activities were then investigated and compared.
29773393	1	20	from	body	354:357	arg1	polysaccharides					284:298	water-soluble H. erinaceus polysaccharides	257:298	water-soluble H. erinaceus polysaccharides (HEPs; HEP-W, HEP-S, HEP-C, and HEP-A) from the fruit body of Hericium erinaceus	257:379	In this study, hot water, 0.9% NaCl, citric acid, and 1.25 M NaOH/0.05% NaBH4 were separately used for the extraction of water-soluble H. erinaceus polysaccharides (HEPs; HEP-W, HEP-S, HEP-C, and HEP-A) from the fruit body of Hericium erinaceus.
29773393	1	20	from	body	354:357	arg1	HEPs					301:304	HEPs	301:304	HEPs	301:304	In this study, hot water, 0.9% NaCl, citric acid, and 1.25 M NaOH/0.05% NaBH4 were separately used for the extraction of water-soluble H. erinaceus polysaccharides (HEPs; HEP-W, HEP-S, HEP-C, and HEP-A) from the fruit body of Hericium erinaceus.
29773393	1	20	from	body	354:357	arg1	HEP-C					321:325	HEP-C	321:325	HEP-C	321:325	In this study, hot water, 0.9% NaCl, citric acid, and 1.25 M NaOH/0.05% NaBH4 were separately used for the extraction of water-soluble H. erinaceus polysaccharides (HEPs; HEP-W, HEP-S, HEP-C, and HEP-A) from the fruit body of Hericium erinaceus.
29773393	1	20	from	body	354:357	arg1	extraction					243:252	the extraction	239:252	the extraction of water-soluble H. erinaceus polysaccharides (HEPs; HEP-W, HEP-S, HEP-C, and HEP-A) from the fruit body of Hericium erinaceus	239:379	In this study, hot water, 0.9% NaCl, citric acid, and 1.25 M NaOH/0.05% NaBH4 were separately used for the extraction of water-soluble H. erinaceus polysaccharides (HEPs; HEP-W, HEP-S, HEP-C, and HEP-A) from the fruit body of Hericium erinaceus.
29773393	1	20	from	body	354:357	arg1	HEP-W					307:311	HEP-W	307:311	HEP-W	307:311	In this study, hot water, 0.9% NaCl, citric acid, and 1.25 M NaOH/0.05% NaBH4 were separately used for the extraction of water-soluble H. erinaceus polysaccharides (HEPs; HEP-W, HEP-S, HEP-C, and HEP-A) from the fruit body of Hericium erinaceus.
29773393	1	20	from	body	354:357	arg1	HEP-A					332:336	HEP-A	332:336	HEP-A	332:336	In this study, hot water, 0.9% NaCl, citric acid, and 1.25 M NaOH/0.05% NaBH4 were separately used for the extraction of water-soluble H. erinaceus polysaccharides (HEPs; HEP-W, HEP-S, HEP-C, and HEP-A) from the fruit body of Hericium erinaceus.
29773393	1	20	from	body	354:357	arg1	HEP-S					314:318	HEP-S	314:318	HEP-S	314:318	In this study, hot water, 0.9% NaCl, citric acid, and 1.25 M NaOH/0.05% NaBH4 were separately used for the extraction of water-soluble H. erinaceus polysaccharides (HEPs; HEP-W, HEP-S, HEP-C, and HEP-A) from the fruit body of Hericium erinaceus.
29773393	1	21	from	extraction	243:252	arg1	body					354:357	the fruit body	344:357	the fruit body of Hericium erinaceus	344:379	In this study, hot water, 0.9% NaCl, citric acid, and 1.25 M NaOH/0.05% NaBH4 were separately used for the extraction of water-soluble H. erinaceus polysaccharides (HEPs; HEP-W, HEP-S, HEP-C, and HEP-A) from the fruit body of Hericium erinaceus.
29773393	4	22	theme	hydroxyl	912:919	arg1	radicals					930:937	hydroxyl and DPPH radicals	912:937	radicals	930:937	In vitro antioxidant activity assays indicated that HEP-C extracted with citric acid solution showed stronger scavenging abilities on hydroxyl and DPPH radicals and antioxidant capacities than HEP-W and HEP-S.
29773393	0	23	theme	Comparative	0:10	arg1	study					12:16	Comparative study	0:16	Comparative study of physicochemical properties	0:46	Comparative study of physicochemical properties and bioactivity of Hericium erinaceus polysaccharides at different solvent extractions.
29773393	4	24	theme	activity	799:806	arg1	assays					808:813	In vitro antioxidant activity assays	778:813	In vitro antioxidant activity assays	778:813	In vitro antioxidant activity assays indicated that HEP-C extracted with citric acid solution showed stronger scavenging abilities on hydroxyl and DPPH radicals and antioxidant capacities than HEP-W and HEP-S.
29773393	1	25	theme	citric	173:178	arg1	acid					180:183	citric acid	173:183	citric acid	173:183	In this study, hot water, 0.9% NaCl, citric acid, and 1.25 M NaOH/0.05% NaBH4 were separately used for the extraction of water-soluble H. erinaceus polysaccharides (HEPs; HEP-W, HEP-S, HEP-C, and HEP-A) from the fruit body of Hericium erinaceus.
29773393	0	26	theme	physicochemical	21:35	arg1	properties					37:46	physicochemical properties	21:46	physicochemical properties	21:46	Comparative study of physicochemical properties and bioactivity of Hericium erinaceus polysaccharides at different solvent extractions.
29773393	3	27	theme	uronic	734:739	arg1	acid					741:744	uronic acid	734:744	uronic acid	734:744	Results showed that the extraction solvents exhibited significant effects on the extraction yields, molecular weights, monosaccharide compositions, preliminary structural characteristics, microstructures of HEPs and on their contents, such as neutral sugar, uronic acid, protein, and β-(1 → 3)-glucan.
29773393	4	28	theme	stronger	879:886	arg1	abilities					899:907	stronger scavenging abilities	879:907	stronger scavenging abilities	879:907	In vitro antioxidant activity assays indicated that HEP-C extracted with citric acid solution showed stronger scavenging abilities on hydroxyl and DPPH radicals and antioxidant capacities than HEP-W and HEP-S.
29773393	4	29	dep	In	778:779	arg1	vitro					781:785	vitro	781:785	vitro	781:785	In vitro antioxidant activity assays indicated that HEP-C extracted with citric acid solution showed stronger scavenging abilities on hydroxyl and DPPH radicals and antioxidant capacities than HEP-W and HEP-S.
29773393	1	30	theme	water-soluble	257:269	arg1	polysaccharides					284:298	water-soluble H. erinaceus polysaccharides	257:298	water-soluble H. erinaceus polysaccharides (HEPs; HEP-W, HEP-S, HEP-C, and HEP-A) from the fruit body of Hericium erinaceus	257:379	In this study, hot water, 0.9% NaCl, citric acid, and 1.25 M NaOH/0.05% NaBH4 were separately used for the extraction of water-soluble H. erinaceus polysaccharides (HEPs; HEP-W, HEP-S, HEP-C, and HEP-A) from the fruit body of Hericium erinaceus.
29773393	1	30	theme	water-soluble	257:269	arg1	HEPs					301:304	HEPs	301:304	HEPs	301:304	In this study, hot water, 0.9% NaCl, citric acid, and 1.25 M NaOH/0.05% NaBH4 were separately used for the extraction of water-soluble H. erinaceus polysaccharides (HEPs; HEP-W, HEP-S, HEP-C, and HEP-A) from the fruit body of Hericium erinaceus.
29773393	1	30	theme	water-soluble	257:269	arg1	HEP-C					321:325	HEP-C	321:325	HEP-C	321:325	In this study, hot water, 0.9% NaCl, citric acid, and 1.25 M NaOH/0.05% NaBH4 were separately used for the extraction of water-soluble H. erinaceus polysaccharides (HEPs; HEP-W, HEP-S, HEP-C, and HEP-A) from the fruit body of Hericium erinaceus.
29773393	1	30	theme	water-soluble	257:269	arg1	HEP-W					307:311	HEP-W	307:311	HEP-W	307:311	In this study, hot water, 0.9% NaCl, citric acid, and 1.25 M NaOH/0.05% NaBH4 were separately used for the extraction of water-soluble H. erinaceus polysaccharides (HEPs; HEP-W, HEP-S, HEP-C, and HEP-A) from the fruit body of Hericium erinaceus.
29773393	1	30	theme	water-soluble	257:269	arg1	HEP-A					332:336	HEP-A	332:336	HEP-A	332:336	In this study, hot water, 0.9% NaCl, citric acid, and 1.25 M NaOH/0.05% NaBH4 were separately used for the extraction of water-soluble H. erinaceus polysaccharides (HEPs; HEP-W, HEP-S, HEP-C, and HEP-A) from the fruit body of Hericium erinaceus.
29773393	1	30	theme	water-soluble	257:269	arg1	HEP-S					314:318	HEP-S	314:318	HEP-S	314:318	In this study, hot water, 0.9% NaCl, citric acid, and 1.25 M NaOH/0.05% NaBH4 were separately used for the extraction of water-soluble H. erinaceus polysaccharides (HEPs; HEP-W, HEP-S, HEP-C, and HEP-A) from the fruit body of Hericium erinaceus.
29773393	3	31	theme	preliminary	624:634	arg1	yields					568:573	the extraction yields	553:573	the extraction yields	553:573	Results showed that the extraction solvents exhibited significant effects on the extraction yields, molecular weights, monosaccharide compositions, preliminary structural characteristics, microstructures of HEPs and on their contents, such as neutral sugar, uronic acid, protein, and β-(1 → 3)-glucan.
29773393	3	31	theme	preliminary	624:634	arg1	characteristics					647:661	preliminary structural characteristics	624:661	preliminary structural characteristics	624:661	Results showed that the extraction solvents exhibited significant effects on the extraction yields, molecular weights, monosaccharide compositions, preliminary structural characteristics, microstructures of HEPs and on their contents, such as neutral sugar, uronic acid, protein, and β-(1 → 3)-glucan.
29773393	3	32	theme	significant	530:540	arg1	effects					542:548	significant effects	530:548	significant effects on the extraction yields, molecular weights, monosaccharide compositions, preliminary structural characteristics, microstructures of HEPs and on their contents, such as neutral sugar, uronic acid, protein, and β-(1 → 3)-glucan	530:775	Results showed that the extraction solvents exhibited significant effects on the extraction yields, molecular weights, monosaccharide compositions, preliminary structural characteristics, microstructures of HEPs and on their contents, such as neutral sugar, uronic acid, protein, and β-(1 → 3)-glucan.
29773393	6	33	dep	food	1207:1210	arg1	industries					1237:1246	industries	1237:1246	industries	1237:1246	Therefore, HEP-C extracted with citric acid can be developed as a potential bioactive ingredient for applications in food, medicine, and cosmetics industries.
29773393	1	34	theme	H.	271:272	arg1	polysaccharides					284:298	water-soluble H. erinaceus polysaccharides	257:298	water-soluble H. erinaceus polysaccharides (HEPs; HEP-W, HEP-S, HEP-C, and HEP-A) from the fruit body of Hericium erinaceus	257:379	In this study, hot water, 0.9% NaCl, citric acid, and 1.25 M NaOH/0.05% NaBH4 were separately used for the extraction of water-soluble H. erinaceus polysaccharides (HEPs; HEP-W, HEP-S, HEP-C, and HEP-A) from the fruit body of Hericium erinaceus.
29773393	1	34	theme	H.	271:272	arg1	HEPs					301:304	HEPs	301:304	HEPs	301:304	In this study, hot water, 0.9% NaCl, citric acid, and 1.25 M NaOH/0.05% NaBH4 were separately used for the extraction of water-soluble H. erinaceus polysaccharides (HEPs; HEP-W, HEP-S, HEP-C, and HEP-A) from the fruit body of Hericium erinaceus.
29773393	1	34	theme	H.	271:272	arg1	HEP-C					321:325	HEP-C	321:325	HEP-C	321:325	In this study, hot water, 0.9% NaCl, citric acid, and 1.25 M NaOH/0.05% NaBH4 were separately used for the extraction of water-soluble H. erinaceus polysaccharides (HEPs; HEP-W, HEP-S, HEP-C, and HEP-A) from the fruit body of Hericium erinaceus.
29773393	1	34	theme	H.	271:272	arg1	HEP-W					307:311	HEP-W	307:311	HEP-W	307:311	In this study, hot water, 0.9% NaCl, citric acid, and 1.25 M NaOH/0.05% NaBH4 were separately used for the extraction of water-soluble H. erinaceus polysaccharides (HEPs; HEP-W, HEP-S, HEP-C, and HEP-A) from the fruit body of Hericium erinaceus.
29773393	1	34	theme	H.	271:272	arg1	HEP-A					332:336	HEP-A	332:336	HEP-A	332:336	In this study, hot water, 0.9% NaCl, citric acid, and 1.25 M NaOH/0.05% NaBH4 were separately used for the extraction of water-soluble H. erinaceus polysaccharides (HEPs; HEP-W, HEP-S, HEP-C, and HEP-A) from the fruit body of Hericium erinaceus.
29773393	1	34	theme	H.	271:272	arg1	HEP-S					314:318	HEP-S	314:318	HEP-S	314:318	In this study, hot water, 0.9% NaCl, citric acid, and 1.25 M NaOH/0.05% NaBH4 were separately used for the extraction of water-soluble H. erinaceus polysaccharides (HEPs; HEP-W, HEP-S, HEP-C, and HEP-A) from the fruit body of Hericium erinaceus.
29773393	3	35	theme	structural	636:645	arg1	yields					568:573	the extraction yields	553:573	the extraction yields	553:573	Results showed that the extraction solvents exhibited significant effects on the extraction yields, molecular weights, monosaccharide compositions, preliminary structural characteristics, microstructures of HEPs and on their contents, such as neutral sugar, uronic acid, protein, and β-(1 → 3)-glucan.
29773393	3	35	theme	structural	636:645	arg1	characteristics					647:661	preliminary structural characteristics	624:661	preliminary structural characteristics	624:661	Results showed that the extraction solvents exhibited significant effects on the extraction yields, molecular weights, monosaccharide compositions, preliminary structural characteristics, microstructures of HEPs and on their contents, such as neutral sugar, uronic acid, protein, and β-(1 → 3)-glucan.
29773393	0	36	theme	properties	37:46	arg1	bioactivity					52:62	bioactivity	52:62	bioactivity of Hericium erinaceus polysaccharides at different solvent extractions	52:133	Comparative study of physicochemical properties and bioactivity of Hericium erinaceus polysaccharides at different solvent extractions.
29773393	0	36	theme	properties	37:46	arg1	study					12:16	Comparative study	0:16	Comparative study of physicochemical properties	0:46	Comparative study of physicochemical properties and bioactivity of Hericium erinaceus polysaccharides at different solvent extractions.
29773393	1	37	theme	erinaceus	274:282	arg1	polysaccharides					284:298	water-soluble H. erinaceus polysaccharides	257:298	water-soluble H. erinaceus polysaccharides (HEPs; HEP-W, HEP-S, HEP-C, and HEP-A) from the fruit body of Hericium erinaceus	257:379	In this study, hot water, 0.9% NaCl, citric acid, and 1.25 M NaOH/0.05% NaBH4 were separately used for the extraction of water-soluble H. erinaceus polysaccharides (HEPs; HEP-W, HEP-S, HEP-C, and HEP-A) from the fruit body of Hericium erinaceus.
29773393	1	37	theme	erinaceus	274:282	arg1	HEPs					301:304	HEPs	301:304	HEPs	301:304	In this study, hot water, 0.9% NaCl, citric acid, and 1.25 M NaOH/0.05% NaBH4 were separately used for the extraction of water-soluble H. erinaceus polysaccharides (HEPs; HEP-W, HEP-S, HEP-C, and HEP-A) from the fruit body of Hericium erinaceus.
29773393	1	37	theme	erinaceus	274:282	arg1	HEP-C					321:325	HEP-C	321:325	HEP-C	321:325	In this study, hot water, 0.9% NaCl, citric acid, and 1.25 M NaOH/0.05% NaBH4 were separately used for the extraction of water-soluble H. erinaceus polysaccharides (HEPs; HEP-W, HEP-S, HEP-C, and HEP-A) from the fruit body of Hericium erinaceus.
29773393	1	37	theme	erinaceus	274:282	arg1	HEP-W					307:311	HEP-W	307:311	HEP-W	307:311	In this study, hot water, 0.9% NaCl, citric acid, and 1.25 M NaOH/0.05% NaBH4 were separately used for the extraction of water-soluble H. erinaceus polysaccharides (HEPs; HEP-W, HEP-S, HEP-C, and HEP-A) from the fruit body of Hericium erinaceus.
29773393	1	37	theme	erinaceus	274:282	arg1	HEP-A					332:336	HEP-A	332:336	HEP-A	332:336	In this study, hot water, 0.9% NaCl, citric acid, and 1.25 M NaOH/0.05% NaBH4 were separately used for the extraction of water-soluble H. erinaceus polysaccharides (HEPs; HEP-W, HEP-S, HEP-C, and HEP-A) from the fruit body of Hericium erinaceus.
29773393	1	37	theme	erinaceus	274:282	arg1	HEP-S					314:318	HEP-S	314:318	HEP-S	314:318	In this study, hot water, 0.9% NaCl, citric acid, and 1.25 M NaOH/0.05% NaBH4 were separately used for the extraction of water-soluble H. erinaceus polysaccharides (HEPs; HEP-W, HEP-S, HEP-C, and HEP-A) from the fruit body of Hericium erinaceus.
29773393	2	38	theme	biological	417:426	arg1	activities					428:437	biological activities	417:437	biological activities	417:437	The physicochemical properties and biological activities were then investigated and compared.
29773393	1	39	used	used	230:233	arg2	water					155:159	hot water	151:159	hot water	151:159	In this study, hot water, 0.9% NaCl, citric acid, and 1.25 M NaOH/0.05% NaBH4 were separately used for the extraction of water-soluble H. erinaceus polysaccharides (HEPs; HEP-W, HEP-S, HEP-C, and HEP-A) from the fruit body of Hericium erinaceus.
29773393	1	39	used	used	230:233	arg2	acid					180:183	citric acid	173:183	citric acid	173:183	In this study, hot water, 0.9% NaCl, citric acid, and 1.25 M NaOH/0.05% NaBH4 were separately used for the extraction of water-soluble H. erinaceus polysaccharides (HEPs; HEP-W, HEP-S, HEP-C, and HEP-A) from the fruit body of Hericium erinaceus.
29773393	1	39	used	used	230:233	arg2	NaCl					167:170	0.9% NaCl	162:170	0.9% NaCl	162:170	In this study, hot water, 0.9% NaCl, citric acid, and 1.25 M NaOH/0.05% NaBH4 were separately used for the extraction of water-soluble H. erinaceus polysaccharides (HEPs; HEP-W, HEP-S, HEP-C, and HEP-A) from the fruit body of Hericium erinaceus.
29773393	1	39	used	used	230:233	arg2	NaBH4					208:212	1.25 M NaOH/0.05% NaBH4	190:212	1.25 M NaOH/0.05% NaBH4	190:212	In this study, hot water, 0.9% NaCl, citric acid, and 1.25 M NaOH/0.05% NaBH4 were separately used for the extraction of water-soluble H. erinaceus polysaccharides (HEPs; HEP-W, HEP-S, HEP-C, and HEP-A) from the fruit body of Hericium erinaceus.
29773393	1	40	theme	1.25 M	190:195	arg1	NaBH4					208:212	1.25 M NaOH/0.05% NaBH4	190:212	1.25 M NaOH/0.05% NaBH4	190:212	In this study, hot water, 0.9% NaCl, citric acid, and 1.25 M NaOH/0.05% NaBH4 were separately used for the extraction of water-soluble H. erinaceus polysaccharides (HEPs; HEP-W, HEP-S, HEP-C, and HEP-A) from the fruit body of Hericium erinaceus.
29773393	1	41	theme	polysaccharides	284:298	arg1	extraction					243:252	the extraction	239:252	the extraction of water-soluble H. erinaceus polysaccharides (HEPs; HEP-W, HEP-S, HEP-C, and HEP-A) from the fruit body of Hericium erinaceus	239:379	In this study, hot water, 0.9% NaCl, citric acid, and 1.25 M NaOH/0.05% NaBH4 were separately used for the extraction of water-soluble H. erinaceus polysaccharides (HEPs; HEP-W, HEP-S, HEP-C, and HEP-A) from the fruit body of Hericium erinaceus.
29773393	4	42	theme	citric	851:856	arg1	solution					863:870	citric acid solution	851:870	citric acid solution	851:870	In vitro antioxidant activity assays indicated that HEP-C extracted with citric acid solution showed stronger scavenging abilities on hydroxyl and DPPH radicals and antioxidant capacities than HEP-W and HEP-S.
29773393	4	43	theme	In	778:779	arg1	assays					808:813	In vitro antioxidant activity assays	778:813	In vitro antioxidant activity assays	778:813	In vitro antioxidant activity assays indicated that HEP-C extracted with citric acid solution showed stronger scavenging abilities on hydroxyl and DPPH radicals and antioxidant capacities than HEP-W and HEP-S.
29773393	4	44	theme	scavenging	888:897	arg1	abilities					899:907	stronger scavenging abilities	879:907	stronger scavenging abilities	879:907	In vitro antioxidant activity assays indicated that HEP-C extracted with citric acid solution showed stronger scavenging abilities on hydroxyl and DPPH radicals and antioxidant capacities than HEP-W and HEP-S.
29773393	1	45	theme	fruit	348:352	arg1	body					354:357	the fruit body	344:357	the fruit body of Hericium erinaceus	344:379	In this study, hot water, 0.9% NaCl, citric acid, and 1.25 M NaOH/0.05% NaBH4 were separately used for the extraction of water-soluble H. erinaceus polysaccharides (HEPs; HEP-W, HEP-S, HEP-C, and HEP-A) from the fruit body of Hericium erinaceus.
29773393	3	46	theme	extraction	557:566	arg1	yields					568:573	the extraction yields	553:573	the extraction yields	553:573	Results showed that the extraction solvents exhibited significant effects on the extraction yields, molecular weights, monosaccharide compositions, preliminary structural characteristics, microstructures of HEPs and on their contents, such as neutral sugar, uronic acid, protein, and β-(1 → 3)-glucan.
29773393	3	46	theme	extraction	557:566	arg1	weights					586:592	molecular weights	576:592	molecular weights	576:592	Results showed that the extraction solvents exhibited significant effects on the extraction yields, molecular weights, monosaccharide compositions, preliminary structural characteristics, microstructures of HEPs and on their contents, such as neutral sugar, uronic acid, protein, and β-(1 → 3)-glucan.
29773393	3	46	theme	extraction	557:566	arg1	compositions					610:621	monosaccharide compositions	595:621	monosaccharide compositions	595:621	Results showed that the extraction solvents exhibited significant effects on the extraction yields, molecular weights, monosaccharide compositions, preliminary structural characteristics, microstructures of HEPs and on their contents, such as neutral sugar, uronic acid, protein, and β-(1 → 3)-glucan.
29773393	3	46	theme	extraction	557:566	arg1	microstructures					664:678	microstructures	664:678	microstructures	664:678	Results showed that the extraction solvents exhibited significant effects on the extraction yields, molecular weights, monosaccharide compositions, preliminary structural characteristics, microstructures of HEPs and on their contents, such as neutral sugar, uronic acid, protein, and β-(1 → 3)-glucan.
29773393	3	46	theme	extraction	557:566	arg1	characteristics					647:661	preliminary structural characteristics	624:661	preliminary structural characteristics	624:661	Results showed that the extraction solvents exhibited significant effects on the extraction yields, molecular weights, monosaccharide compositions, preliminary structural characteristics, microstructures of HEPs and on their contents, such as neutral sugar, uronic acid, protein, and β-(1 → 3)-glucan.
29773393	5	47	theme	α-glycosidase	1050:1062	arg1	activities					1078:1087	α-glycosidase and α-amylase activities	1050:1087	α-glycosidase and α-amylase activities	1050:1087	Moreover, HEP-C exhibited the strongest inhibitory effects on α-glycosidase and α-amylase activities.
29773393	0	48	from	extractions	123:133	arg1	bioactivity					52:62	bioactivity	52:62	bioactivity of Hericium erinaceus polysaccharides at different solvent extractions	52:133	Comparative study of physicochemical properties and bioactivity of Hericium erinaceus polysaccharides at different solvent extractions.
29773393	0	48	from	extractions	123:133	arg1	study					12:16	Comparative study	0:16	Comparative study of physicochemical properties	0:46	Comparative study of physicochemical properties and bioactivity of Hericium erinaceus polysaccharides at different solvent extractions.
29773393	1	49	theme	NaOH/0.05	197:205	arg1	NaBH4					208:212	1.25 M NaOH/0.05% NaBH4	190:212	1.25 M NaOH/0.05% NaBH4	190:212	In this study, hot water, 0.9% NaCl, citric acid, and 1.25 M NaOH/0.05% NaBH4 were separately used for the extraction of water-soluble H. erinaceus polysaccharides (HEPs; HEP-W, HEP-S, HEP-C, and HEP-A) from the fruit body of Hericium erinaceus.
29773393	4	50	theme	acid	858:861	arg1	solution					863:870	citric acid solution	851:870	citric acid solution	851:870	In vitro antioxidant activity assays indicated that HEP-C extracted with citric acid solution showed stronger scavenging abilities on hydroxyl and DPPH radicals and antioxidant capacities than HEP-W and HEP-S.
29773393	5	51	theme	strongest	1018:1026	arg1	effects					1039:1045	the strongest inhibitory effects	1014:1045	the strongest inhibitory effects on α-glycosidase and α-amylase activities	1014:1087	Moreover, HEP-C exhibited the strongest inhibitory effects on α-glycosidase and α-amylase activities.
29773393	3	52	theme	monosaccharide	595:608	arg1	yields					568:573	the extraction yields	553:573	the extraction yields	553:573	Results showed that the extraction solvents exhibited significant effects on the extraction yields, molecular weights, monosaccharide compositions, preliminary structural characteristics, microstructures of HEPs and on their contents, such as neutral sugar, uronic acid, protein, and β-(1 → 3)-glucan.
29773393	3	52	theme	monosaccharide	595:608	arg1	compositions					610:621	monosaccharide compositions	595:621	monosaccharide compositions	595:621	Results showed that the extraction solvents exhibited significant effects on the extraction yields, molecular weights, monosaccharide compositions, preliminary structural characteristics, microstructures of HEPs and on their contents, such as neutral sugar, uronic acid, protein, and β-(1 → 3)-glucan.
29773393	3	53	from	effects	542:548	arg1	yields					568:573	the extraction yields	553:573	the extraction yields	553:573	Results showed that the extraction solvents exhibited significant effects on the extraction yields, molecular weights, monosaccharide compositions, preliminary structural characteristics, microstructures of HEPs and on their contents, such as neutral sugar, uronic acid, protein, and β-(1 → 3)-glucan.
29773393	3	53	from	effects	542:548	arg1	compositions					610:621	monosaccharide compositions	595:621	monosaccharide compositions	595:621	Results showed that the extraction solvents exhibited significant effects on the extraction yields, molecular weights, monosaccharide compositions, preliminary structural characteristics, microstructures of HEPs and on their contents, such as neutral sugar, uronic acid, protein, and β-(1 → 3)-glucan.
29773393	3	53	from	effects	542:548	arg1	acid					741:744	uronic acid	734:744	uronic acid	734:744	Results showed that the extraction solvents exhibited significant effects on the extraction yields, molecular weights, monosaccharide compositions, preliminary structural characteristics, microstructures of HEPs and on their contents, such as neutral sugar, uronic acid, protein, and β-(1 → 3)-glucan.
29773393	3	53	from	effects	542:548	arg1	-glucan					769:775	β-(1 → 3)-glucan	760:775	β-(1 → 3)-glucan	760:775	Results showed that the extraction solvents exhibited significant effects on the extraction yields, molecular weights, monosaccharide compositions, preliminary structural characteristics, microstructures of HEPs and on their contents, such as neutral sugar, uronic acid, protein, and β-(1 → 3)-glucan.
29773393	3	53	from	effects	542:548	arg1	protein					747:753	protein	747:753	protein	747:753	Results showed that the extraction solvents exhibited significant effects on the extraction yields, molecular weights, monosaccharide compositions, preliminary structural characteristics, microstructures of HEPs and on their contents, such as neutral sugar, uronic acid, protein, and β-(1 → 3)-glucan.
29773393	3	53	from	effects	542:548	arg1	weights					586:592	molecular weights	576:592	molecular weights	576:592	Results showed that the extraction solvents exhibited significant effects on the extraction yields, molecular weights, monosaccharide compositions, preliminary structural characteristics, microstructures of HEPs and on their contents, such as neutral sugar, uronic acid, protein, and β-(1 → 3)-glucan.
29773393	3	53	from	effects	542:548	arg1	sugar					727:731	neutral sugar	719:731	neutral sugar	719:731	Results showed that the extraction solvents exhibited significant effects on the extraction yields, molecular weights, monosaccharide compositions, preliminary structural characteristics, microstructures of HEPs and on their contents, such as neutral sugar, uronic acid, protein, and β-(1 → 3)-glucan.
29773393	3	53	from	effects	542:548	arg1	characteristics					647:661	preliminary structural characteristics	624:661	preliminary structural characteristics	624:661	Results showed that the extraction solvents exhibited significant effects on the extraction yields, molecular weights, monosaccharide compositions, preliminary structural characteristics, microstructures of HEPs and on their contents, such as neutral sugar, uronic acid, protein, and β-(1 → 3)-glucan.
29773393	3	53	from	effects	542:548	arg1	microstructures					664:678	microstructures	664:678	microstructures	664:678	Results showed that the extraction solvents exhibited significant effects on the extraction yields, molecular weights, monosaccharide compositions, preliminary structural characteristics, microstructures of HEPs and on their contents, such as neutral sugar, uronic acid, protein, and β-(1 → 3)-glucan.
29773393	3	53	from	effects	542:548	arg1	contents					701:708	their contents	695:708	their contents	695:708	Results showed that the extraction solvents exhibited significant effects on the extraction yields, molecular weights, monosaccharide compositions, preliminary structural characteristics, microstructures of HEPs and on their contents, such as neutral sugar, uronic acid, protein, and β-(1 → 3)-glucan.
29773393	1	54	theme	%	206:206	arg1	NaBH4					208:212	1.25 M NaOH/0.05% NaBH4	190:212	1.25 M NaOH/0.05% NaBH4	190:212	In this study, hot water, 0.9% NaCl, citric acid, and 1.25 M NaOH/0.05% NaBH4 were separately used for the extraction of water-soluble H. erinaceus polysaccharides (HEPs; HEP-W, HEP-S, HEP-C, and HEP-A) from the fruit body of Hericium erinaceus.
29773393	0	55	theme	erinaceus	76:84	arg1	polysaccharides					86:100	Hericium erinaceus polysaccharides	67:100	Hericium erinaceus polysaccharides	67:100	Comparative study of physicochemical properties and bioactivity of Hericium erinaceus polysaccharides at different solvent extractions.
29773393	1	56	theme	Hericium	362:369	arg1	erinaceus					371:379	Hericium erinaceus	362:379	Hericium erinaceus	362:379	In this study, hot water, 0.9% NaCl, citric acid, and 1.25 M NaOH/0.05% NaBH4 were separately used for the extraction of water-soluble H. erinaceus polysaccharides (HEPs; HEP-W, HEP-S, HEP-C, and HEP-A) from the fruit body of Hericium erinaceus.
29773393	3	57	theme	molecular	576:584	arg1	yields					568:573	the extraction yields	553:573	the extraction yields	553:573	Results showed that the extraction solvents exhibited significant effects on the extraction yields, molecular weights, monosaccharide compositions, preliminary structural characteristics, microstructures of HEPs and on their contents, such as neutral sugar, uronic acid, protein, and β-(1 → 3)-glucan.
29773393	3	57	theme	molecular	576:584	arg1	weights					586:592	molecular weights	576:592	molecular weights	576:592	Results showed that the extraction solvents exhibited significant effects on the extraction yields, molecular weights, monosaccharide compositions, preliminary structural characteristics, microstructures of HEPs and on their contents, such as neutral sugar, uronic acid, protein, and β-(1 → 3)-glucan.
29773393	1	58	theme	hot	151:153	arg1	water					155:159	hot water	151:159	hot water	151:159	In this study, hot water, 0.9% NaCl, citric acid, and 1.25 M NaOH/0.05% NaBH4 were separately used for the extraction of water-soluble H. erinaceus polysaccharides (HEPs; HEP-W, HEP-S, HEP-C, and HEP-A) from the fruit body of Hericium erinaceus.
29773393	0	59	theme	Hericium	67:74	arg1	polysaccharides					86:100	Hericium erinaceus polysaccharides	67:100	Hericium erinaceus polysaccharides	67:100	Comparative study of physicochemical properties and bioactivity of Hericium erinaceus polysaccharides at different solvent extractions.
29773393	6	60	theme	bioactive	1166:1174	arg1	HEP-C					1101:1105	HEP-C	1101:1105	HEP-C extracted with citric acid	1101:1132	Therefore, HEP-C extracted with citric acid can be developed as a potential bioactive ingredient for applications in food, medicine, and cosmetics industries.
29773393	6	60	theme	bioactive	1166:1174	arg1	ingredient					1176:1185	a potential bioactive ingredient	1154:1185	a potential bioactive ingredient for applications in food, medicine, and cosmetics industries	1154:1246	Therefore, HEP-C extracted with citric acid can be developed as a potential bioactive ingredient for applications in food, medicine, and cosmetics industries.
29773393	6	61	theme	potential	1156:1164	arg1	HEP-C					1101:1105	HEP-C	1101:1105	HEP-C extracted with citric acid	1101:1132	Therefore, HEP-C extracted with citric acid can be developed as a potential bioactive ingredient for applications in food, medicine, and cosmetics industries.
29773393	6	61	theme	potential	1156:1164	arg1	ingredient					1176:1185	a potential bioactive ingredient	1154:1185	a potential bioactive ingredient for applications in food, medicine, and cosmetics industries	1154:1246	Therefore, HEP-C extracted with citric acid can be developed as a potential bioactive ingredient for applications in food, medicine, and cosmetics industries.
29773393	3	62	theme	β-	760:761	arg1	-glucan					769:775	β-(1 → 3)-glucan	760:775	β-(1 → 3)-glucan	760:775	Results showed that the extraction solvents exhibited significant effects on the extraction yields, molecular weights, monosaccharide compositions, preliminary structural characteristics, microstructures of HEPs and on their contents, such as neutral sugar, uronic acid, protein, and β-(1 → 3)-glucan.
29343853	0	0	theme	Aggrecan	78:85	arg1	gene					87:90	the Aggrecan gene	74:90	the Aggrecan gene	74:90	Differential tissue specific, temporal and spatial expression patterns of the Aggrecan gene is modulated by independent enhancer elements.
29343853	2	1	from	tissue	446:451	arg1	organisation					396:407	the organisation	392:407	the organisation of the chondrocytes in cartilaginous tissue	392:451	The central aim of this study was to identify whether transcriptional enhancers played a role in the organisation of the chondrocytes in cartilaginous tissue.
29343853	9	2	theme	precise	1369:1375	arg1	expression					1392:1401	the precise spatiotemporal expression	1365:1401	the precise spatiotemporal expression of this gene in cartilage types	1365:1433	Thus, the Acan enhancers orchestrate the precise spatiotemporal expression of this gene in cartilage types at different stages of development and adulthood.
29343853	5	3	theme	adult	897:901	arg1	cartilage					903:911	adult cartilage	897:911	adult cartilage	897:911	In particular, one enhancer (-80) distinguishes itself from the others by being predominantly active in adult cartilage.
29343853	5	4	from	active	887:892	arg1	cartilage					903:911	adult cartilage	897:911	adult cartilage	897:911	In particular, one enhancer (-80) distinguishes itself from the others by being predominantly active in adult cartilage.
29343853	5	5	from	cartilage	903:911	arg1	active					887:892	active	887:892	active	887:892	In particular, one enhancer (-80) distinguishes itself from the others by being predominantly active in adult cartilage.
29343853	4	6	theme	stage	774:778	arg1	specificity					780:790	individual chondrocyte developmental stage specificity	737:790	individual chondrocyte developmental stage specificity	737:790	Using transgenic reporter studies in mice we identified four elements, two of which showed individual chondrocyte developmental stage specificity.
29343853	9	7	theme	gene	1411:1414	arg1	expression					1392:1401	the precise spatiotemporal expression	1365:1401	the precise spatiotemporal expression of this gene in cartilage types	1365:1433	Thus, the Acan enhancers orchestrate the precise spatiotemporal expression of this gene in cartilage types at different stages of development and adulthood.
29343853	0	8	theme	gene	87:90	arg1	patterns					62:69	Differential tissue specific, temporal and spatial expression patterns	0:69	Differential tissue specific, temporal and spatial expression patterns of the Aggrecan gene	0:90	Differential tissue specific, temporal and spatial expression patterns of the Aggrecan gene is modulated by independent enhancer elements.
29343853	4	9	theme	developmental	760:772	arg1	specificity					780:790	individual chondrocyte developmental stage specificity	737:790	individual chondrocyte developmental stage specificity	737:790	Using transgenic reporter studies in mice we identified four elements, two of which showed individual chondrocyte developmental stage specificity.
29343853	4	10	dep	identified	691:700	arg1	Using					646:650	Using	646:650	Using transgenic reporter studies in mice	646:686	Using transgenic reporter studies in mice we identified four elements, two of which showed individual chondrocyte developmental stage specificity.
29343853	2	11	theme	central	299:305	arg1	aim					307:309	The central aim	295:309	The central aim of this study	295:323	The central aim of this study was to identify whether transcriptional enhancers played a role in the organisation of the chondrocytes in cartilaginous tissue.
29343853	9	12	theme	adulthood	1474:1482	arg1	stages					1448:1453	different stages	1438:1453	different stages of development and adulthood	1438:1482	Thus, the Acan enhancers orchestrate the precise spatiotemporal expression of this gene in cartilage types at different stages of development and adulthood.
29343853	4	13	from	studies	672:678	arg1	mice					683:686	mice	683:686	mice	683:686	Using transgenic reporter studies in mice we identified four elements, two of which showed individual chondrocyte developmental stage specificity.
29343853	4	14	theme	reporter	663:670	arg1	studies					672:678	transgenic reporter studies	652:678	transgenic reporter studies in mice	652:686	Using transgenic reporter studies in mice we identified four elements, two of which showed individual chondrocyte developmental stage specificity.
29343853	8	15	from	one	1211:1213	arg1	mutation					1177:1184	mutation	1177:1184	mutation of SOX9 binding sites in one of the enhancers (-30)	1177:1236	The transcription factor SOX9 interacted with all the enhancers in vitro and mutation of SOX9 binding sites in one of the enhancers (-30) resulted in a loss of its chondrocyte specificity and ectopic enhancer reporter activity.
29343853	3	16	theme	normal	520:525	arg1	structure					527:535	normal structure	520:535	normal structure	520:535	We focused on the Aggrecan gene (Acan) as it is essential for the normal structure and function of cartilage and it is expressed developmentally in different stages of chondrocyte maturation.
29343853	4	17	theme	transgenic	652:661	arg1	studies					672:678	transgenic reporter studies	652:678	transgenic reporter studies in mice	652:686	Using transgenic reporter studies in mice we identified four elements, two of which showed individual chondrocyte developmental stage specificity.
29343853	8	18	theme	SOX9	1189:1192	arg1	sites					1202:1206	SOX9 binding sites	1189:1206	SOX9 binding sites in one of the enhancers	1189:1230	The transcription factor SOX9 interacted with all the enhancers in vitro and mutation of SOX9 binding sites in one of the enhancers (-30) resulted in a loss of its chondrocyte specificity and ectopic enhancer reporter activity.
29343853	7	19	theme	chondrocyte	1082:1092	arg1	type					1094:1097	chondrocyte type	1082:1097	chondrocyte type	1082:1097	The remaining chondrocyte specific enhancers, +28 and -30, showed no preference to chondrocyte type.
29343853	3	20	theme	cartilage	553:561	arg1	structure					527:535	normal structure	520:535	normal structure	520:535	We focused on the Aggrecan gene (Acan) as it is essential for the normal structure and function of cartilage and it is expressed developmentally in different stages of chondrocyte maturation.
29343853	3	20	theme	cartilage	553:561	arg1	function					541:548	function	541:548	function	541:548	We focused on the Aggrecan gene (Acan) as it is essential for the normal structure and function of cartilage and it is expressed developmentally in different stages of chondrocyte maturation.
29343853	9	21	from	expression	1392:1401	arg1	types					1429:1433	cartilage types	1419:1433	cartilage types	1419:1433	Thus, the Acan enhancers orchestrate the precise spatiotemporal expression of this gene in cartilage types at different stages of development and adulthood.
29343853	0	22	theme	Differential	0:11	arg1	patterns					62:69	Differential tissue specific, temporal and spatial expression patterns	0:69	Differential tissue specific, temporal and spatial expression patterns of the Aggrecan gene	0:90	Differential tissue specific, temporal and spatial expression patterns of the Aggrecan gene is modulated by independent enhancer elements.
29343853	6	23	theme	early	979:983	arg1	chondrocytes					985:996	early chondrocytes	979:996	early chondrocytes	979:996	Furthermore, the -62 element uniquely drove reporter activity in early chondrocytes.
29343853	2	24	theme	study	319:323	arg1	aim					307:309	The central aim	295:309	The central aim of this study	295:323	The central aim of this study was to identify whether transcriptional enhancers played a role in the organisation of the chondrocytes in cartilaginous tissue.
29343853	9	25	theme	different	1438:1446	arg1	stages					1448:1453	different stages	1438:1453	different stages of development and adulthood	1438:1482	Thus, the Acan enhancers orchestrate the precise spatiotemporal expression of this gene in cartilage types at different stages of development and adulthood.
29343853	8	26	theme	sites	1202:1206	arg1	mutation					1177:1184	mutation	1177:1184	mutation of SOX9 binding sites in one of the enhancers (-30)	1177:1236	The transcription factor SOX9 interacted with all the enhancers in vitro and mutation of SOX9 binding sites in one of the enhancers (-30) resulted in a loss of its chondrocyte specificity and ectopic enhancer reporter activity.
29343853	0	27	theme	enhancer	120:127	arg1	elements					129:136	independent enhancer elements	108:136	independent enhancer elements	108:136	Differential tissue specific, temporal and spatial expression patterns of the Aggrecan gene is modulated by independent enhancer elements.
29343853	4	28	theme	chondrocyte	748:758	arg1	specificity					780:790	individual chondrocyte developmental stage specificity	737:790	individual chondrocyte developmental stage specificity	737:790	Using transgenic reporter studies in mice we identified four elements, two of which showed individual chondrocyte developmental stage specificity.
29343853	2	29	theme	cartilaginous	432:444	arg1	tissue					446:451	cartilaginous tissue	432:451	cartilaginous tissue	432:451	The central aim of this study was to identify whether transcriptional enhancers played a role in the organisation of the chondrocytes in cartilaginous tissue.
29343853	0	30	theme	specific	20:27	arg1	patterns					62:69	Differential tissue specific, temporal and spatial expression patterns	0:69	Differential tissue specific, temporal and spatial expression patterns of the Aggrecan gene	0:90	Differential tissue specific, temporal and spatial expression patterns of the Aggrecan gene is modulated by independent enhancer elements.
29343853	8	31	from	mutation	1177:1184	arg1	one					1211:1213	one	1211:1213	one	1211:1213	The transcription factor SOX9 interacted with all the enhancers in vitro and mutation of SOX9 binding sites in one of the enhancers (-30) resulted in a loss of its chondrocyte specificity and ectopic enhancer reporter activity.
29343853	8	31	from	mutation	1177:1184	arg1	enhancers					1222:1230	the enhancers	1218:1230	the enhancers	1218:1230	The transcription factor SOX9 interacted with all the enhancers in vitro and mutation of SOX9 binding sites in one of the enhancers (-30) resulted in a loss of its chondrocyte specificity and ectopic enhancer reporter activity.
29343853	0	32	theme	independent	108:118	arg1	elements					129:136	independent enhancer elements	108:136	independent enhancer elements	108:136	Differential tissue specific, temporal and spatial expression patterns of the Aggrecan gene is modulated by independent enhancer elements.
29343853	4	33	theme	individual	737:746	arg1	specificity					780:790	individual chondrocyte developmental stage specificity	737:790	individual chondrocyte developmental stage specificity	737:790	Using transgenic reporter studies in mice we identified four elements, two of which showed individual chondrocyte developmental stage specificity.
29343853	6	34	theme	reporter	958:965	arg1	activity					967:974	reporter activity	958:974	reporter activity	958:974	Furthermore, the -62 element uniquely drove reporter activity in early chondrocytes.
29343853	1	35	theme	temporal	220:227	arg1	composition					229:239	the spatial and temporal composition	204:239	the spatial and temporal composition of the cartilage tissue	204:263	The transcriptional mechanism through which chondrocytes control the spatial and temporal composition of the cartilage tissue has remained largely elusive.
29343853	8	36	theme	binding	1194:1200	arg1	sites					1202:1206	SOX9 binding sites	1189:1206	SOX9 binding sites in one of the enhancers	1189:1230	The transcription factor SOX9 interacted with all the enhancers in vitro and mutation of SOX9 binding sites in one of the enhancers (-30) resulted in a loss of its chondrocyte specificity and ectopic enhancer reporter activity.
29343853	2	37	theme	chondrocytes	416:427	arg1	organisation					396:407	the organisation	392:407	the organisation of the chondrocytes in cartilaginous tissue	392:451	The central aim of this study was to identify whether transcriptional enhancers played a role in the organisation of the chondrocytes in cartilaginous tissue.
29343853	9	38	theme	cartilage	1419:1427	arg1	types					1429:1433	cartilage types	1419:1433	cartilage types	1419:1433	Thus, the Acan enhancers orchestrate the precise spatiotemporal expression of this gene in cartilage types at different stages of development and adulthood.
29343853	3	39	theme	chondrocyte	622:632	arg1	maturation					634:643	chondrocyte maturation	622:643	chondrocyte maturation	622:643	We focused on the Aggrecan gene (Acan) as it is essential for the normal structure and function of cartilage and it is expressed developmentally in different stages of chondrocyte maturation.
29343853	8	40	dep	factor	1118:1123	arg1	SOX9					1125:1128	SOX9	1125:1128	The transcription factor SOX9	1100:1128	The transcription factor SOX9 interacted with all the enhancers in vitro and mutation of SOX9 binding sites in one of the enhancers (-30) resulted in a loss of its chondrocyte specificity and ectopic enhancer reporter activity.
29343853	0	41	theme	expression	51:60	arg1	patterns					62:69	Differential tissue specific, temporal and spatial expression patterns	0:69	Differential tissue specific, temporal and spatial expression patterns of the Aggrecan gene	0:90	Differential tissue specific, temporal and spatial expression patterns of the Aggrecan gene is modulated by independent enhancer elements.
29343853	9	42	theme	Acan	1338:1341	arg1	enhancers					1343:1351	the Acan enhancers	1334:1351	the Acan enhancers	1334:1351	Thus, the Acan enhancers orchestrate the precise spatiotemporal expression of this gene in cartilage types at different stages of development and adulthood.
29343853	3	43	theme	maturation	634:643	arg1	stages					612:617	different stages	602:617	different stages of chondrocyte maturation	602:643	We focused on the Aggrecan gene (Acan) as it is essential for the normal structure and function of cartilage and it is expressed developmentally in different stages of chondrocyte maturation.
29343853	9	44	theme	development	1458:1468	arg1	stages					1448:1453	different stages	1438:1453	different stages of development and adulthood	1438:1482	Thus, the Acan enhancers orchestrate the precise spatiotemporal expression of this gene in cartilage types at different stages of development and adulthood.
29343853	2	45	theme	transcriptional	349:363	arg1	enhancers					365:373	transcriptional enhancers	349:373	transcriptional enhancers	349:373	The central aim of this study was to identify whether transcriptional enhancers played a role in the organisation of the chondrocytes in cartilaginous tissue.
29343853	8	46	theme	transcription	1104:1116	arg1	factor					1118:1123	The transcription factor	1100:1123	The transcription factor SOX9	1100:1128	The transcription factor SOX9 interacted with all the enhancers in vitro and mutation of SOX9 binding sites in one of the enhancers (-30) resulted in a loss of its chondrocyte specificity and ectopic enhancer reporter activity.
29343853	8	47	theme	enhancer	1300:1307	arg1	activity					1318:1325	ectopic enhancer reporter activity	1292:1325	ectopic enhancer reporter activity	1292:1325	The transcription factor SOX9 interacted with all the enhancers in vitro and mutation of SOX9 binding sites in one of the enhancers (-30) resulted in a loss of its chondrocyte specificity and ectopic enhancer reporter activity.
29343853	7	48	theme	chondrocyte	1013:1023	arg1	-30					1053:1055	-30	1053:1055	-30	1053:1055	The remaining chondrocyte specific enhancers, +28 and -30, showed no preference to chondrocyte type.
29343853	7	48	theme	chondrocyte	1013:1023	arg1	+28					1045:1047	+28	1045:1047	+28	1045:1047	The remaining chondrocyte specific enhancers, +28 and -30, showed no preference to chondrocyte type.
29343853	7	48	theme	chondrocyte	1013:1023	arg1	enhancers					1034:1042	The remaining chondrocyte specific enhancers	999:1042	The remaining chondrocyte specific enhancers	999:1042	The remaining chondrocyte specific enhancers, +28 and -30, showed no preference to chondrocyte type.
29343853	4	49	theme	elements	707:714	arg1	two					717:719	two	717:719	two	717:719	Using transgenic reporter studies in mice we identified four elements, two of which showed individual chondrocyte developmental stage specificity.
29343853	4	49	theme	elements	707:714	arg1	elements					707:714	four elements	702:714	four elements	702:714	Using transgenic reporter studies in mice we identified four elements, two of which showed individual chondrocyte developmental stage specificity.
29343853	7	50	theme	remaining	1003:1011	arg1	-30					1053:1055	-30	1053:1055	-30	1053:1055	The remaining chondrocyte specific enhancers, +28 and -30, showed no preference to chondrocyte type.
29343853	7	50	theme	remaining	1003:1011	arg1	+28					1045:1047	+28	1045:1047	+28	1045:1047	The remaining chondrocyte specific enhancers, +28 and -30, showed no preference to chondrocyte type.
29343853	7	50	theme	remaining	1003:1011	arg1	enhancers					1034:1042	The remaining chondrocyte specific enhancers	999:1042	The remaining chondrocyte specific enhancers	999:1042	The remaining chondrocyte specific enhancers, +28 and -30, showed no preference to chondrocyte type.
29343853	2	51	from	organisation	396:407	arg1	tissue					446:451	cartilaginous tissue	432:451	cartilaginous tissue	432:451	The central aim of this study was to identify whether transcriptional enhancers played a role in the organisation of the chondrocytes in cartilaginous tissue.
29343853	3	52	dep	structure	527:535	arg1	the					516:518	the	516:518	the	516:518	We focused on the Aggrecan gene (Acan) as it is essential for the normal structure and function of cartilage and it is expressed developmentally in different stages of chondrocyte maturation.
29343853	7	53	theme	specific	1025:1032	arg1	-30					1053:1055	-30	1053:1055	-30	1053:1055	The remaining chondrocyte specific enhancers, +28 and -30, showed no preference to chondrocyte type.
29343853	7	53	theme	specific	1025:1032	arg1	+28					1045:1047	+28	1045:1047	+28	1045:1047	The remaining chondrocyte specific enhancers, +28 and -30, showed no preference to chondrocyte type.
29343853	7	53	theme	specific	1025:1032	arg1	enhancers					1034:1042	The remaining chondrocyte specific enhancers	999:1042	The remaining chondrocyte specific enhancers	999:1042	The remaining chondrocyte specific enhancers, +28 and -30, showed no preference to chondrocyte type.
29343853	0	54	dep	specific	20:27	arg1	spatial					43:49	spatial	43:49	spatial	43:49	Differential tissue specific, temporal and spatial expression patterns of the Aggrecan gene is modulated by independent enhancer elements.
29343853	0	54	dep	specific	20:27	arg1	temporal					30:37	temporal	30:37	temporal	30:37	Differential tissue specific, temporal and spatial expression patterns of the Aggrecan gene is modulated by independent enhancer elements.
29343853	2	55	from	chondrocytes	416:427	arg1	tissue					446:451	cartilaginous tissue	432:451	cartilaginous tissue	432:451	The central aim of this study was to identify whether transcriptional enhancers played a role in the organisation of the chondrocytes in cartilaginous tissue.
29343853	8	56	theme	chondrocyte	1264:1274	arg1	specificity					1276:1286	its chondrocyte specificity	1260:1286	its chondrocyte specificity	1260:1286	The transcription factor SOX9 interacted with all the enhancers in vitro and mutation of SOX9 binding sites in one of the enhancers (-30) resulted in a loss of its chondrocyte specificity and ectopic enhancer reporter activity.
29343853	8	57	theme	reporter	1309:1316	arg1	activity					1318:1325	ectopic enhancer reporter activity	1292:1325	ectopic enhancer reporter activity	1292:1325	The transcription factor SOX9 interacted with all the enhancers in vitro and mutation of SOX9 binding sites in one of the enhancers (-30) resulted in a loss of its chondrocyte specificity and ectopic enhancer reporter activity.
29343853	8	58	theme	specificity	1276:1286	arg1	activity					1318:1325	ectopic enhancer reporter activity	1292:1325	ectopic enhancer reporter activity	1292:1325	The transcription factor SOX9 interacted with all the enhancers in vitro and mutation of SOX9 binding sites in one of the enhancers (-30) resulted in a loss of its chondrocyte specificity and ectopic enhancer reporter activity.
29343853	8	58	theme	specificity	1276:1286	arg1	loss					1252:1255	a loss	1250:1255	a loss of its chondrocyte specificity	1250:1286	The transcription factor SOX9 interacted with all the enhancers in vitro and mutation of SOX9 binding sites in one of the enhancers (-30) resulted in a loss of its chondrocyte specificity and ectopic enhancer reporter activity.
29343853	1	59	theme	spatial	208:214	arg1	composition					229:239	the spatial and temporal composition	204:239	the spatial and temporal composition of the cartilage tissue	204:263	The transcriptional mechanism through which chondrocytes control the spatial and temporal composition of the cartilage tissue has remained largely elusive.
29343853	3	60	theme	Aggrecan	472:479	arg1	Acan					487:490	Acan	487:490	Acan	487:490	We focused on the Aggrecan gene (Acan) as it is essential for the normal structure and function of cartilage and it is expressed developmentally in different stages of chondrocyte maturation.
29343853	3	60	theme	Aggrecan	472:479	arg1	gene					481:484	the Aggrecan gene	468:484	the Aggrecan gene (Acan)	468:491	We focused on the Aggrecan gene (Acan) as it is essential for the normal structure and function of cartilage and it is expressed developmentally in different stages of chondrocyte maturation.
29343853	8	61	theme	ectopic	1292:1298	arg1	activity					1318:1325	ectopic enhancer reporter activity	1292:1325	ectopic enhancer reporter activity	1292:1325	The transcription factor SOX9 interacted with all the enhancers in vitro and mutation of SOX9 binding sites in one of the enhancers (-30) resulted in a loss of its chondrocyte specificity and ectopic enhancer reporter activity.
29343853	8	62	theme	enhancers	1222:1230	arg1	one					1211:1213	one	1211:1213	one	1211:1213	The transcription factor SOX9 interacted with all the enhancers in vitro and mutation of SOX9 binding sites in one of the enhancers (-30) resulted in a loss of its chondrocyte specificity and ectopic enhancer reporter activity.
29343853	8	62	theme	enhancers	1222:1230	arg1	enhancers					1222:1230	the enhancers	1218:1230	the enhancers	1218:1230	The transcription factor SOX9 interacted with all the enhancers in vitro and mutation of SOX9 binding sites in one of the enhancers (-30) resulted in a loss of its chondrocyte specificity and ectopic enhancer reporter activity.
29343853	1	63	theme	cartilage	248:256	arg1	tissue					258:263	the cartilage tissue	244:263	the cartilage tissue	244:263	The transcriptional mechanism through which chondrocytes control the spatial and temporal composition of the cartilage tissue has remained largely elusive.
29343853	9	64	theme	spatiotemporal	1377:1390	arg1	expression					1392:1401	the precise spatiotemporal expression	1365:1401	the precise spatiotemporal expression of this gene in cartilage types	1365:1433	Thus, the Acan enhancers orchestrate the precise spatiotemporal expression of this gene in cartilage types at different stages of development and adulthood.
29343853	8	65	from	sites	1202:1206	arg1	one					1211:1213	one	1211:1213	one	1211:1213	The transcription factor SOX9 interacted with all the enhancers in vitro and mutation of SOX9 binding sites in one of the enhancers (-30) resulted in a loss of its chondrocyte specificity and ectopic enhancer reporter activity.
29343853	8	65	from	sites	1202:1206	arg1	enhancers					1222:1230	the enhancers	1218:1230	the enhancers	1218:1230	The transcription factor SOX9 interacted with all the enhancers in vitro and mutation of SOX9 binding sites in one of the enhancers (-30) resulted in a loss of its chondrocyte specificity and ectopic enhancer reporter activity.
29343853	3	66	theme	different	602:610	arg1	stages					612:617	different stages	602:617	different stages of chondrocyte maturation	602:643	We focused on the Aggrecan gene (Acan) as it is essential for the normal structure and function of cartilage and it is expressed developmentally in different stages of chondrocyte maturation.
29343853	1	67	theme	transcriptional	143:157	arg1	mechanism					159:167	The transcriptional mechanism	139:167	The transcriptional mechanism through which chondrocytes control the spatial and temporal composition of the cartilage tissue	139:263	The transcriptional mechanism through which chondrocytes control the spatial and temporal composition of the cartilage tissue has remained largely elusive.
29343853	1	68	theme	tissue	258:263	arg1	composition					229:239	the spatial and temporal composition	204:239	the spatial and temporal composition of the cartilage tissue	204:263	The transcriptional mechanism through which chondrocytes control the spatial and temporal composition of the cartilage tissue has remained largely elusive.
31877961	0	0	theme	Diet	72:75	arg1	Effects					53:59	the Detrimental Effects	37:59	the Detrimental Effects of Western Diet in a Colitis-Associated Colorectal Cancer Model	37:123	Switching to a Healthy Diet Prevents the Detrimental Effects of Western Diet in a Colitis-Associated Colorectal Cancer Model.
31877961	6	1	theme	whole	831:835	arg1	period					850:855	the whole experimental period	827:855	the whole experimental period	827:855	Feeding the WD during the whole experimental period aggravated colitis symptoms, shortened the colon (p < 0.05), changed microbiota composition and increased tumor promotion.
31877961	8	2	theme	longer	1191:1196	arg1	foci					1269:1272	fewer (p < 0.05) and smaller (p < 0.01) aberrant colonic crypt foci	1206:1272	fewer (p < 0.05) and smaller (p < 0.01) aberrant colonic crypt foci	1206:1272	The switch to the AIN93G diet ameliorated this effect, reflected by longer colons, fewer (p < 0.05) and smaller (p < 0.01) aberrant colonic crypt foci, comparable with the AIN group.
31877961	8	2	theme	longer	1191:1196	arg1	colons					1198:1203	longer colons	1191:1203	longer colons	1191:1203	The switch to the AIN93G diet ameliorated this effect, reflected by longer colons, fewer (p < 0.05) and smaller (p < 0.01) aberrant colonic crypt foci, comparable with the AIN group.
31877961	8	3	theme	colonic	1255:1261	arg1	foci					1269:1272	fewer (p < 0.05) and smaller (p < 0.01) aberrant colonic crypt foci	1206:1272	fewer (p < 0.05) and smaller (p < 0.01) aberrant colonic crypt foci	1206:1272	The switch to the AIN93G diet ameliorated this effect, reflected by longer colons, fewer (p < 0.05) and smaller (p < 0.01) aberrant colonic crypt foci, comparable with the AIN group.
31877961	8	3	theme	colonic	1255:1261	arg1	colons					1198:1203	longer colons	1191:1203	longer colons	1191:1203	The switch to the AIN93G diet ameliorated this effect, reflected by longer colons, fewer (p < 0.05) and smaller (p < 0.01) aberrant colonic crypt foci, comparable with the AIN group.
31877961	0	4	theme	Western	64:70	arg1	Diet					72:75	Western Diet	64:75	Western Diet	64:75	Switching to a Healthy Diet Prevents the Detrimental Effects of Western Diet in a Colitis-Associated Colorectal Cancer Model.
31877961	7	5	theme	p	1030:1030	arg1	proliferation					1015:1027	proliferation	1015:1027	proliferation (p < 0.05)	1015:1038	On molecular level, the WD reduced proliferation (p < 0.05) and increased expression of the vitamin D catabolizing enzyme Cyp24a1 (p < 0.001).
31877961	7	5	theme	p	1030:1030	arg1	<					1032:1032	p < 0.05	1030:1037	p < 0.05	1030:1037	On molecular level, the WD reduced proliferation (p < 0.05) and increased expression of the vitamin D catabolizing enzyme Cyp24a1 (p < 0.001).
31877961	3	6	theme	WD	512:513	arg1	WD					508:509	WD	508:509	WD (WD group)	508:520	Female BALB/c mice received either WD (WD group) or standard AIN93G diet (AIN group) for the whole experimental period.
31877961	3	6	theme	WD	512:513	arg1	group					515:519	WD group	512:519	WD group	512:519	Female BALB/c mice received either WD (WD group) or standard AIN93G diet (AIN group) for the whole experimental period.
31877961	7	7	theme	catabolizing	1082:1093	arg1	Cyp24a1					1102:1108	the vitamin D catabolizing enzyme Cyp24a1	1068:1108	the vitamin D catabolizing enzyme Cyp24a1 (p < 0.001)	1068:1120	On molecular level, the WD reduced proliferation (p < 0.05) and increased expression of the vitamin D catabolizing enzyme Cyp24a1 (p < 0.001).
31877961	7	7	theme	catabolizing	1082:1093	arg1	<					1113:1113	p < 0.001	1111:1119	p < 0.001	1111:1119	On molecular level, the WD reduced proliferation (p < 0.05) and increased expression of the vitamin D catabolizing enzyme Cyp24a1 (p < 0.001).
31877961	9	8	theme	deleterious	1408:1418	arg1	effect					1420:1425	the deleterious effect	1404:1425	the deleterious effect of the WD	1404:1435	Our results show that switching to a healthy diet, even after cancer initiation is able to revert the deleterious effect of the WD and could be an effective preventive strategy to reduce colitis symptoms and prevent tumorigenesis.
31877961	8	9	with	comparable	1275:1284	arg1	group					1299:1303	the AIN group	1291:1303	the AIN group	1291:1303	The switch to the AIN93G diet ameliorated this effect, reflected by longer colons, fewer (p < 0.05) and smaller (p < 0.01) aberrant colonic crypt foci, comparable with the AIN group.
31877961	8	10	theme	p	1236:1236	arg1	<					1238:1238	p < 0.01	1236:1243	p < 0.01	1236:1243	The switch to the AIN93G diet ameliorated this effect, reflected by longer colons, fewer (p < 0.05) and smaller (p < 0.01) aberrant colonic crypt foci, comparable with the AIN group.
31877961	3	11	theme	AIN93G	534:539	arg1	diet					541:544	standard AIN93G diet	525:544	standard AIN93G diet (AIN group)	525:556	Female BALB/c mice received either WD (WD group) or standard AIN93G diet (AIN group) for the whole experimental period.
31877961	3	11	theme	AIN93G	534:539	arg1	group					551:555	AIN group	547:555	AIN group	547:555	Female BALB/c mice received either WD (WD group) or standard AIN93G diet (AIN group) for the whole experimental period.
31877961	6	12	theme	p	907:907	arg1	<					909:909	p < 0.05	907:914	p < 0.05	907:914	Feeding the WD during the whole experimental period aggravated colitis symptoms, shortened the colon (p < 0.05), changed microbiota composition and increased tumor promotion.
31877961	6	12	theme	p	907:907	arg1	colon					900:904	the colon	896:904	the colon (p < 0.05)	896:915	Feeding the WD during the whole experimental period aggravated colitis symptoms, shortened the colon (p < 0.05), changed microbiota composition and increased tumor promotion.
31877961	7	13	theme	enzyme	1095:1100	arg1	Cyp24a1					1102:1108	the vitamin D catabolizing enzyme Cyp24a1	1068:1108	the vitamin D catabolizing enzyme Cyp24a1 (p < 0.001)	1068:1120	On molecular level, the WD reduced proliferation (p < 0.05) and increased expression of the vitamin D catabolizing enzyme Cyp24a1 (p < 0.001).
31877961	7	13	theme	enzyme	1095:1100	arg1	<					1113:1113	p < 0.001	1111:1119	p < 0.001	1111:1119	On molecular level, the WD reduced proliferation (p < 0.05) and increased expression of the vitamin D catabolizing enzyme Cyp24a1 (p < 0.001).
31877961	9	14	theme	healthy	1343:1349	arg1	diet					1351:1354	a healthy diet	1341:1354	a healthy diet	1341:1354	Our results show that switching to a healthy diet, even after cancer initiation is able to revert the deleterious effect of the WD and could be an effective preventive strategy to reduce colitis symptoms and prevent tumorigenesis.
31877961	8	15	dep	smaller	1227:1233	arg1	<					1238:1238	p < 0.01	1236:1243	p < 0.01	1236:1243	The switch to the AIN93G diet ameliorated this effect, reflected by longer colons, fewer (p < 0.05) and smaller (p < 0.01) aberrant colonic crypt foci, comparable with the AIN group.
31877961	0	16	theme	Colorectal	101:110	arg1	Model					119:123	a Colitis-Associated Colorectal Cancer Model	80:123	a Colitis-Associated Colorectal Cancer Model	80:123	Switching to a Healthy Diet Prevents the Detrimental Effects of Western Diet in a Colitis-Associated Colorectal Cancer Model.
31877961	4	17	theme	DSS	681:683	arg1	cycles					685:690	three DSS cycles	675:690	three DSS cycles	675:690	After five weeks, the mice received 12.5 mg/kg AOM intraperitoneally, followed by three DSS cycles.
31877961	5	18	theme	first	773:777	arg1	cycle					783:787	the first DSS cycle	769:787	the first DSS cycle (WD/AIN group)	769:802	In one group of mice, the WD was switched to AIN93G the day before starting the first DSS cycle (WD/AIN group).
31877961	5	18	theme	first	773:777	arg1	group					797:801	WD/AIN group	790:801	WD/AIN group	790:801	In one group of mice, the WD was switched to AIN93G the day before starting the first DSS cycle (WD/AIN group).
31877961	2	19	theme	AIN93G	398:403	arg1	diet					405:408	the standard AIN93G diet	385:408	the standard AIN93G diet	385:408	We hypothesized that Western-style diet (WD) aggravates azoxymethane (AOM)/dextran sulfate sodium salt (DSS)-induced colitis-associated tumorigenesis and that switching to the standard AIN93G diet will ameliorate disease symptoms even after cancer initiation.
31877961	0	20	theme	Colitis-Associated	82:99	arg1	Model					119:123	a Colitis-Associated Colorectal Cancer Model	80:123	a Colitis-Associated Colorectal Cancer Model	80:123	Switching to a Healthy Diet Prevents the Detrimental Effects of Western Diet in a Colitis-Associated Colorectal Cancer Model.
31877961	7	21	theme	molecular	983:991	arg1	level					993:997	molecular level	983:997	molecular level	983:997	On molecular level, the WD reduced proliferation (p < 0.05) and increased expression of the vitamin D catabolizing enzyme Cyp24a1 (p < 0.001).
31877961	3	22	theme	standard	525:532	arg1	diet					541:544	standard AIN93G diet	525:544	standard AIN93G diet (AIN group)	525:556	Female BALB/c mice received either WD (WD group) or standard AIN93G diet (AIN group) for the whole experimental period.
31877961	3	22	theme	standard	525:532	arg1	group					551:555	AIN group	547:555	AIN group	547:555	Female BALB/c mice received either WD (WD group) or standard AIN93G diet (AIN group) for the whole experimental period.
31877961	2	23	theme	standard	389:396	arg1	diet					405:408	the standard AIN93G diet	385:408	the standard AIN93G diet	385:408	We hypothesized that Western-style diet (WD) aggravates azoxymethane (AOM)/dextran sulfate sodium salt (DSS)-induced colitis-associated tumorigenesis and that switching to the standard AIN93G diet will ameliorate disease symptoms even after cancer initiation.
31877961	2	24	theme	cancer	454:459	arg1	initiation					461:470	cancer initiation	454:470	cancer initiation	454:470	We hypothesized that Western-style diet (WD) aggravates azoxymethane (AOM)/dextran sulfate sodium salt (DSS)-induced colitis-associated tumorigenesis and that switching to the standard AIN93G diet will ameliorate disease symptoms even after cancer initiation.
31877961	9	25	theme	WD	1434:1435	arg1	effect					1420:1425	the deleterious effect	1404:1425	the deleterious effect of the WD	1404:1435	Our results show that switching to a healthy diet, even after cancer initiation is able to revert the deleterious effect of the WD and could be an effective preventive strategy to reduce colitis symptoms and prevent tumorigenesis.
31877961	7	26	theme	p	1111:1111	arg1	Cyp24a1					1102:1108	the vitamin D catabolizing enzyme Cyp24a1	1068:1108	the vitamin D catabolizing enzyme Cyp24a1 (p < 0.001)	1068:1120	On molecular level, the WD reduced proliferation (p < 0.05) and increased expression of the vitamin D catabolizing enzyme Cyp24a1 (p < 0.001).
31877961	7	26	theme	p	1111:1111	arg1	<					1113:1113	p < 0.001	1111:1119	p < 0.001	1111:1119	On molecular level, the WD reduced proliferation (p < 0.05) and increased expression of the vitamin D catabolizing enzyme Cyp24a1 (p < 0.001).
31877961	9	27	theme	effective	1453:1461	arg1	strategy					1474:1481	an effective preventive strategy	1450:1481	an effective preventive strategy to reduce colitis symptoms and prevent tumorigenesis	1450:1534	Our results show that switching to a healthy diet, even after cancer initiation is able to revert the deleterious effect of the WD and could be an effective preventive strategy to reduce colitis symptoms and prevent tumorigenesis.
31877961	9	28	theme	preventive	1463:1472	arg1	strategy					1474:1481	an effective preventive strategy	1450:1481	an effective preventive strategy to reduce colitis symptoms and prevent tumorigenesis	1450:1534	Our results show that switching to a healthy diet, even after cancer initiation is able to revert the deleterious effect of the WD and could be an effective preventive strategy to reduce colitis symptoms and prevent tumorigenesis.
31877961	8	29	theme	p	1213:1213	arg1	<					1215:1215	p < 0.05	1213:1220	p < 0.05	1213:1220	The switch to the AIN93G diet ameliorated this effect, reflected by longer colons, fewer (p < 0.05) and smaller (p < 0.01) aberrant colonic crypt foci, comparable with the AIN group.
31877961	1	30	theme	colitis-associated	186:203	arg1	cancer					205:210	colitis-associated cancer	186:210	colitis-associated cancer	186:210	Inflammatory bowel disease increases the odds of developing colitis-associated cancer.
31877961	0	31	theme	Healthy	15:21	arg1	Diet					23:26	a Healthy Diet	13:26	a Healthy Diet	13:26	Switching to a Healthy Diet Prevents the Detrimental Effects of Western Diet in a Colitis-Associated Colorectal Cancer Model.
31877961	9	32	theme	cancer	1368:1373	arg1	initiation					1375:1384	cancer initiation	1368:1384	cancer initiation	1368:1384	Our results show that switching to a healthy diet, even after cancer initiation is able to revert the deleterious effect of the WD and could be an effective preventive strategy to reduce colitis symptoms and prevent tumorigenesis.
31877961	8	33	theme	smaller	1227:1233	arg1	foci					1269:1272	fewer (p < 0.05) and smaller (p < 0.01) aberrant colonic crypt foci	1206:1272	fewer (p < 0.05) and smaller (p < 0.01) aberrant colonic crypt foci	1206:1272	The switch to the AIN93G diet ameliorated this effect, reflected by longer colons, fewer (p < 0.05) and smaller (p < 0.01) aberrant colonic crypt foci, comparable with the AIN group.
31877961	8	33	theme	smaller	1227:1233	arg1	colons					1198:1203	longer colons	1191:1203	longer colons	1191:1203	The switch to the AIN93G diet ameliorated this effect, reflected by longer colons, fewer (p < 0.05) and smaller (p < 0.01) aberrant colonic crypt foci, comparable with the AIN group.
31877961	2	34	theme	disease	426:432	arg1	symptoms					434:441	disease symptoms	426:441	disease symptoms	426:441	We hypothesized that Western-style diet (WD) aggravates azoxymethane (AOM)/dextran sulfate sodium salt (DSS)-induced colitis-associated tumorigenesis and that switching to the standard AIN93G diet will ameliorate disease symptoms even after cancer initiation.
31877961	0	35	theme	Cancer	112:117	arg1	Model					119:123	a Colitis-Associated Colorectal Cancer Model	80:123	a Colitis-Associated Colorectal Cancer Model	80:123	Switching to a Healthy Diet Prevents the Detrimental Effects of Western Diet in a Colitis-Associated Colorectal Cancer Model.
31877961	8	36	theme	crypt	1263:1267	arg1	foci					1269:1272	fewer (p < 0.05) and smaller (p < 0.01) aberrant colonic crypt foci	1206:1272	fewer (p < 0.05) and smaller (p < 0.01) aberrant colonic crypt foci	1206:1272	The switch to the AIN93G diet ameliorated this effect, reflected by longer colons, fewer (p < 0.05) and smaller (p < 0.01) aberrant colonic crypt foci, comparable with the AIN group.
31877961	8	36	theme	crypt	1263:1267	arg1	colons					1198:1203	longer colons	1191:1203	longer colons	1191:1203	The switch to the AIN93G diet ameliorated this effect, reflected by longer colons, fewer (p < 0.05) and smaller (p < 0.01) aberrant colonic crypt foci, comparable with the AIN group.
31877961	3	37	theme	AIN	547:549	arg1	diet					541:544	standard AIN93G diet	525:544	standard AIN93G diet (AIN group)	525:556	Female BALB/c mice received either WD (WD group) or standard AIN93G diet (AIN group) for the whole experimental period.
31877961	3	37	theme	AIN	547:549	arg1	group					551:555	AIN group	547:555	AIN group	547:555	Female BALB/c mice received either WD (WD group) or standard AIN93G diet (AIN group) for the whole experimental period.
31877961	3	38	theme	BALB/c	480:485	arg1	mice					487:490	Female BALB/c mice	473:490	Female BALB/c mice	473:490	Female BALB/c mice received either WD (WD group) or standard AIN93G diet (AIN group) for the whole experimental period.
31877961	7	39	theme	vitamin	1072:1078	arg1	Cyp24a1					1102:1108	the vitamin D catabolizing enzyme Cyp24a1	1068:1108	the vitamin D catabolizing enzyme Cyp24a1 (p < 0.001)	1068:1120	On molecular level, the WD reduced proliferation (p < 0.05) and increased expression of the vitamin D catabolizing enzyme Cyp24a1 (p < 0.001).
31877961	7	39	theme	vitamin	1072:1078	arg1	<					1113:1113	p < 0.001	1111:1119	p < 0.001	1111:1119	On molecular level, the WD reduced proliferation (p < 0.05) and increased expression of the vitamin D catabolizing enzyme Cyp24a1 (p < 0.001).
31877961	8	40	theme	AIN93G	1141:1146	arg1	diet					1148:1151	the AIN93G diet	1137:1151	the AIN93G diet	1137:1151	The switch to the AIN93G diet ameliorated this effect, reflected by longer colons, fewer (p < 0.05) and smaller (p < 0.01) aberrant colonic crypt foci, comparable with the AIN group.
31877961	3	41	theme	whole	566:570	arg1	period					585:590	the whole experimental period	562:590	the whole experimental period	562:590	Female BALB/c mice received either WD (WD group) or standard AIN93G diet (AIN group) for the whole experimental period.
31877961	4	42	theme	mg/kg	634:638	arg1	AOM					640:642	12.5 mg/kg AOM	629:642	12.5 mg/kg AOM	629:642	After five weeks, the mice received 12.5 mg/kg AOM intraperitoneally, followed by three DSS cycles.
31877961	5	43	theme	DSS	779:781	arg1	cycle					783:787	the first DSS cycle	769:787	the first DSS cycle (WD/AIN group)	769:802	In one group of mice, the WD was switched to AIN93G the day before starting the first DSS cycle (WD/AIN group).
31877961	5	43	theme	DSS	779:781	arg1	group					797:801	WD/AIN group	790:801	WD/AIN group	790:801	In one group of mice, the WD was switched to AIN93G the day before starting the first DSS cycle (WD/AIN group).
31877961	7	44	theme	Cyp24a1	1102:1108	arg1	proliferation					1015:1027	proliferation	1015:1027	proliferation (p < 0.05)	1015:1038	On molecular level, the WD reduced proliferation (p < 0.05) and increased expression of the vitamin D catabolizing enzyme Cyp24a1 (p < 0.001).
31877961	7	44	theme	Cyp24a1	1102:1108	arg1	<					1032:1032	p < 0.05	1030:1037	p < 0.05	1030:1037	On molecular level, the WD reduced proliferation (p < 0.05) and increased expression of the vitamin D catabolizing enzyme Cyp24a1 (p < 0.001).
31877961	7	44	theme	Cyp24a1	1102:1108	arg1	expression					1054:1063	increased expression	1044:1063	increased expression of the vitamin D catabolizing enzyme Cyp24a1 (p < 0.001)	1044:1120	On molecular level, the WD reduced proliferation (p < 0.05) and increased expression of the vitamin D catabolizing enzyme Cyp24a1 (p < 0.001).
31877961	8	45	dep	fewer	1206:1210	arg1	<					1215:1215	p < 0.05	1213:1220	p < 0.05	1213:1220	The switch to the AIN93G diet ameliorated this effect, reflected by longer colons, fewer (p < 0.05) and smaller (p < 0.01) aberrant colonic crypt foci, comparable with the AIN group.
31877961	9	46	theme	colitis	1493:1499	arg1	symptoms					1501:1508	colitis symptoms	1493:1508	colitis symptoms	1493:1508	Our results show that switching to a healthy diet, even after cancer initiation is able to revert the deleterious effect of the WD and could be an effective preventive strategy to reduce colitis symptoms and prevent tumorigenesis.
31877961	3	47	theme	experimental	572:583	arg1	period					585:590	the whole experimental period	562:590	the whole experimental period	562:590	Female BALB/c mice received either WD (WD group) or standard AIN93G diet (AIN group) for the whole experimental period.
31877961	7	48	theme	D	1080:1080	arg1	Cyp24a1					1102:1108	the vitamin D catabolizing enzyme Cyp24a1	1068:1108	the vitamin D catabolizing enzyme Cyp24a1 (p < 0.001)	1068:1120	On molecular level, the WD reduced proliferation (p < 0.05) and increased expression of the vitamin D catabolizing enzyme Cyp24a1 (p < 0.001).
31877961	7	48	theme	D	1080:1080	arg1	<					1113:1113	p < 0.001	1111:1119	p < 0.001	1111:1119	On molecular level, the WD reduced proliferation (p < 0.05) and increased expression of the vitamin D catabolizing enzyme Cyp24a1 (p < 0.001).
31877961	0	49	theme	Detrimental	41:51	arg1	Effects					53:59	the Detrimental Effects	37:59	the Detrimental Effects of Western Diet in a Colitis-Associated Colorectal Cancer Model	37:123	Switching to a Healthy Diet Prevents the Detrimental Effects of Western Diet in a Colitis-Associated Colorectal Cancer Model.
31877961	6	50	theme	colitis	868:874	arg1	symptoms					876:883	colitis symptoms	868:883	colitis symptoms	868:883	Feeding the WD during the whole experimental period aggravated colitis symptoms, shortened the colon (p < 0.05), changed microbiota composition and increased tumor promotion.
31877961	0	51	from	Effects	53:59	arg1	Model					119:123	a Colitis-Associated Colorectal Cancer Model	80:123	a Colitis-Associated Colorectal Cancer Model	80:123	Switching to a Healthy Diet Prevents the Detrimental Effects of Western Diet in a Colitis-Associated Colorectal Cancer Model.
31877961	2	52	theme	Western-style	234:246	arg1	diet					248:251	Western-style diet	234:251	Western-style diet (WD)	234:256	We hypothesized that Western-style diet (WD) aggravates azoxymethane (AOM)/dextran sulfate sodium salt (DSS)-induced colitis-associated tumorigenesis and that switching to the standard AIN93G diet will ameliorate disease symptoms even after cancer initiation.
31877961	2	52	theme	Western-style	234:246	arg1	WD					254:255	WD	254:255	WD	254:255	We hypothesized that Western-style diet (WD) aggravates azoxymethane (AOM)/dextran sulfate sodium salt (DSS)-induced colitis-associated tumorigenesis and that switching to the standard AIN93G diet will ameliorate disease symptoms even after cancer initiation.
31877961	2	53	theme	colitis-associated	330:347	arg1	tumorigenesis					349:361	azoxymethane (AOM)/dextran sulfate sodium salt (DSS)-induced colitis-associated tumorigenesis	269:361	azoxymethane (AOM)/dextran sulfate sodium salt (DSS)-induced colitis-associated tumorigenesis	269:361	We hypothesized that Western-style diet (WD) aggravates azoxymethane (AOM)/dextran sulfate sodium salt (DSS)-induced colitis-associated tumorigenesis and that switching to the standard AIN93G diet will ameliorate disease symptoms even after cancer initiation.
31877961	2	54	theme	-induced	321:328	arg1	tumorigenesis					349:361	azoxymethane (AOM)/dextran sulfate sodium salt (DSS)-induced colitis-associated tumorigenesis	269:361	azoxymethane (AOM)/dextran sulfate sodium salt (DSS)-induced colitis-associated tumorigenesis	269:361	We hypothesized that Western-style diet (WD) aggravates azoxymethane (AOM)/dextran sulfate sodium salt (DSS)-induced colitis-associated tumorigenesis and that switching to the standard AIN93G diet will ameliorate disease symptoms even after cancer initiation.
31877961	7	55	theme	increased	1044:1052	arg1	expression					1054:1063	increased expression	1044:1063	increased expression of the vitamin D catabolizing enzyme Cyp24a1 (p < 0.001)	1044:1120	On molecular level, the WD reduced proliferation (p < 0.05) and increased expression of the vitamin D catabolizing enzyme Cyp24a1 (p < 0.001).
31877961	8	56	theme	comparable	1275:1284	arg1	foci					1269:1272	fewer (p < 0.05) and smaller (p < 0.01) aberrant colonic crypt foci	1206:1272	fewer (p < 0.05) and smaller (p < 0.01) aberrant colonic crypt foci	1206:1272	The switch to the AIN93G diet ameliorated this effect, reflected by longer colons, fewer (p < 0.05) and smaller (p < 0.01) aberrant colonic crypt foci, comparable with the AIN group.
31877961	8	56	theme	comparable	1275:1284	arg1	colons					1198:1203	longer colons	1191:1203	longer colons	1191:1203	The switch to the AIN93G diet ameliorated this effect, reflected by longer colons, fewer (p < 0.05) and smaller (p < 0.01) aberrant colonic crypt foci, comparable with the AIN group.
31877961	6	57	theme	microbiota	926:935	arg1	composition					937:947	microbiota composition	926:947	microbiota composition	926:947	Feeding the WD during the whole experimental period aggravated colitis symptoms, shortened the colon (p < 0.05), changed microbiota composition and increased tumor promotion.
31877961	8	58	theme	fewer	1206:1210	arg1	foci					1269:1272	fewer (p < 0.05) and smaller (p < 0.01) aberrant colonic crypt foci	1206:1272	fewer (p < 0.05) and smaller (p < 0.01) aberrant colonic crypt foci	1206:1272	The switch to the AIN93G diet ameliorated this effect, reflected by longer colons, fewer (p < 0.05) and smaller (p < 0.01) aberrant colonic crypt foci, comparable with the AIN group.
31877961	8	58	theme	fewer	1206:1210	arg1	colons					1198:1203	longer colons	1191:1203	longer colons	1191:1203	The switch to the AIN93G diet ameliorated this effect, reflected by longer colons, fewer (p < 0.05) and smaller (p < 0.01) aberrant colonic crypt foci, comparable with the AIN group.
31877961	8	59	theme	AIN	1295:1297	arg1	group					1299:1303	the AIN group	1291:1303	the AIN group	1291:1303	The switch to the AIN93G diet ameliorated this effect, reflected by longer colons, fewer (p < 0.05) and smaller (p < 0.01) aberrant colonic crypt foci, comparable with the AIN group.
31877961	6	60	theme	tumor	963:967	arg1	promotion					969:977	tumor promotion	963:977	tumor promotion	963:977	Feeding the WD during the whole experimental period aggravated colitis symptoms, shortened the colon (p < 0.05), changed microbiota composition and increased tumor promotion.
31877961	1	61	theme	Inflammatory	126:137	arg1	disease					145:151	Inflammatory bowel disease	126:151	Inflammatory bowel disease	126:151	Inflammatory bowel disease increases the odds of developing colitis-associated cancer.
31877961	5	62	theme	mice	709:712	arg1	group					700:704	one group	696:704	one group of mice	696:712	In one group of mice, the WD was switched to AIN93G the day before starting the first DSS cycle (WD/AIN group).
31877961	5	62	theme	mice	709:712	arg1	mice					709:712	mice	709:712	mice	709:712	In one group of mice, the WD was switched to AIN93G the day before starting the first DSS cycle (WD/AIN group).
31877961	8	63	theme	aberrant	1246:1253	arg1	foci					1269:1272	fewer (p < 0.05) and smaller (p < 0.01) aberrant colonic crypt foci	1206:1272	fewer (p < 0.05) and smaller (p < 0.01) aberrant colonic crypt foci	1206:1272	The switch to the AIN93G diet ameliorated this effect, reflected by longer colons, fewer (p < 0.05) and smaller (p < 0.01) aberrant colonic crypt foci, comparable with the AIN group.
31877961	8	63	theme	aberrant	1246:1253	arg1	colons					1198:1203	longer colons	1191:1203	longer colons	1191:1203	The switch to the AIN93G diet ameliorated this effect, reflected by longer colons, fewer (p < 0.05) and smaller (p < 0.01) aberrant colonic crypt foci, comparable with the AIN group.
31877961	3	64	theme	Female	473:478	arg1	mice					487:490	Female BALB/c mice	473:490	Female BALB/c mice	473:490	Female BALB/c mice received either WD (WD group) or standard AIN93G diet (AIN group) for the whole experimental period.
31877961	1	65	theme	bowel	139:143	arg1	disease					145:151	Inflammatory bowel disease	126:151	Inflammatory bowel disease	126:151	Inflammatory bowel disease increases the odds of developing colitis-associated cancer.
31877961	5	66	theme	WD/AIN	790:795	arg1	cycle					783:787	the first DSS cycle	769:787	the first DSS cycle (WD/AIN group)	769:802	In one group of mice, the WD was switched to AIN93G the day before starting the first DSS cycle (WD/AIN group).
31877961	5	66	theme	WD/AIN	790:795	arg1	group					797:801	WD/AIN group	790:801	WD/AIN group	790:801	In one group of mice, the WD was switched to AIN93G the day before starting the first DSS cycle (WD/AIN group).
31877961	6	67	theme	experimental	837:848	arg1	period					850:855	the whole experimental period	827:855	the whole experimental period	827:855	Feeding the WD during the whole experimental period aggravated colitis symptoms, shortened the colon (p < 0.05), changed microbiota composition and increased tumor promotion.
30591340	1	0	theme	rumen-degradable	347:362	arg1	RDP					373:375	RDP	373:375	RDP	373:375	Our objectives were to determine the effects of readily rumen-available carbohydrate source (refined starch vs. dextrose), the level of rumen-degradable protein (RDP), and their interaction on lactation performance, ruminal measurements, enteric methane (CH4) emission, nutrient digestibility, and nitrogen (N) balance in lactating dairy cows.
30591340	1	0	theme	rumen-degradable	347:362	arg1	protein					364:370	rumen-degradable protein	347:370	rumen-degradable protein (RDP)	347:376	Our objectives were to determine the effects of readily rumen-available carbohydrate source (refined starch vs. dextrose), the level of rumen-degradable protein (RDP), and their interaction on lactation performance, ruminal measurements, enteric methane (CH4) emission, nutrient digestibility, and nitrogen (N) balance in lactating dairy cows.
30591340	11	1	theme	N	2293:2293	arg1	balance					2295:2301	N balance	2293:2301	N balance	2293:2301	Reducing the level of RDP in iso-nitrogenous diets had no effect on nutrient apparent total-tract digestibility, manure excretion and composition, N balance, and CH4 production.
30591340	8	2	theme	N	1592:1592	arg1	efficiency					1598:1607	N use efficiency	1592:1607	N use efficiency (milk N/intake N)	1592:1625	Replacing refined starch with dextrose increased DMI, the molar percentage of ruminal butyrate and valerate, daily CH4 production (g/d), and fecal N and decreased the molar percentage of ruminal branched-chain volatile fatty acids, feed efficiency (fat- and protein-corrected milk/DMI), and N use efficiency (milk N/intake N) but did not influence nutrient digestibility.
30591340	8	2	theme	N	1592:1592	arg1	N					1624:1624	milk N/intake N	1610:1624	milk N/intake N	1610:1624	Replacing refined starch with dextrose increased DMI, the molar percentage of ruminal butyrate and valerate, daily CH4 production (g/d), and fecal N and decreased the molar percentage of ruminal branched-chain volatile fatty acids, feed efficiency (fat- and protein-corrected milk/DMI), and N use efficiency (milk N/intake N) but did not influence nutrient digestibility.
30591340	12	3	theme	CH4/d	2494:2498	arg1	g					2489:2489	g	2489:2489	g of CH4/d	2489:2498	In this study, treatments did not affect yield (20.0 g of CH4/kg of DMI) or intensity (13.1 g of CH4/kg of fat- and protein-corrected milk), but methane production (g of CH4/d) was 7.0% lower and N use efficiency (conversion of intake N into milk protein) was 7.8% higher for cows fed a diet of 28.1% starch and 4.6% water-soluble carbohydrate compared with diets with lower starch and higher water-soluble carbohydrate contents.
30591340	7	4	theme	dry	1224:1226	arg1	DMI					1243:1245	DMI	1243:1245	DMI	1243:1245	Carbohydrate source × RDP level interaction tended to influence dry matter intake (DMI), the concentration of urinary N, and urinary urea-N.
30591340	7	4	theme	dry	1224:1226	arg1	intake					1235:1240	dry matter intake	1224:1240	dry matter intake (DMI)	1224:1246	Carbohydrate source × RDP level interaction tended to influence dry matter intake (DMI), the concentration of urinary N, and urinary urea-N.
30591340	12	5	theme	starch	2625:2630	arg1	diet					2611:2614	a diet	2609:2614	a diet of 28.1% starch	2609:2630	In this study, treatments did not affect yield (20.0 g of CH4/kg of DMI) or intensity (13.1 g of CH4/kg of fat- and protein-corrected milk), but methane production (g of CH4/d) was 7.0% lower and N use efficiency (conversion of intake N into milk protein) was 7.8% higher for cows fed a diet of 28.1% starch and 4.6% water-soluble carbohydrate compared with diets with lower starch and higher water-soluble carbohydrate contents.
30591340	2	6	theme	Holstein	590:597	arg1	cows					599:602	Eighteen mid-lactation multiparous Holstein cows	555:602	Eighteen mid-lactation multiparous Holstein cows	555:602	Eighteen mid-lactation multiparous Holstein cows were used in this split-plot study.
30591340	8	7	theme	milk	1610:1613	arg1	efficiency					1598:1607	N use efficiency	1592:1607	N use efficiency (milk N/intake N)	1592:1625	Replacing refined starch with dextrose increased DMI, the molar percentage of ruminal butyrate and valerate, daily CH4 production (g/d), and fecal N and decreased the molar percentage of ruminal branched-chain volatile fatty acids, feed efficiency (fat- and protein-corrected milk/DMI), and N use efficiency (milk N/intake N) but did not influence nutrient digestibility.
30591340	8	7	theme	milk	1610:1613	arg1	N					1624:1624	milk N/intake N	1610:1624	milk N/intake N	1610:1624	Replacing refined starch with dextrose increased DMI, the molar percentage of ruminal butyrate and valerate, daily CH4 production (g/d), and fecal N and decreased the molar percentage of ruminal branched-chain volatile fatty acids, feed efficiency (fat- and protein-corrected milk/DMI), and N use efficiency (milk N/intake N) but did not influence nutrient digestibility.
30591340	1	8	theme	ruminal	427:433	arg1	measurements					435:446	ruminal measurements	427:446	ruminal measurements	427:446	Our objectives were to determine the effects of readily rumen-available carbohydrate source (refined starch vs. dextrose), the level of rumen-degradable protein (RDP), and their interaction on lactation performance, ruminal measurements, enteric methane (CH4) emission, nutrient digestibility, and nitrogen (N) balance in lactating dairy cows.
30591340	12	9	theme	CH4/kg	2421:2426	arg1	g					2416:2416	13.1 g	2411:2416	13.1 g of CH4/kg of fat- and protein-corrected milk	2411:2461	In this study, treatments did not affect yield (20.0 g of CH4/kg of DMI) or intensity (13.1 g of CH4/kg of fat- and protein-corrected milk), but methane production (g of CH4/d) was 7.0% lower and N use efficiency (conversion of intake N into milk protein) was 7.8% higher for cows fed a diet of 28.1% starch and 4.6% water-soluble carbohydrate compared with diets with lower starch and higher water-soluble carbohydrate contents.
30591340	2	10	theme	mid-lactation	564:576	arg1	cows					599:602	Eighteen mid-lactation multiparous Holstein cows	555:602	Eighteen mid-lactation multiparous Holstein cows	555:602	Eighteen mid-lactation multiparous Holstein cows were used in this split-plot study.
30591340	12	11	dep	production	2477:2486	arg1	g					2489:2489	g	2489:2489	g of CH4/d	2489:2498	In this study, treatments did not affect yield (20.0 g of CH4/kg of DMI) or intensity (13.1 g of CH4/kg of fat- and protein-corrected milk), but methane production (g of CH4/d) was 7.0% lower and N use efficiency (conversion of intake N into milk protein) was 7.8% higher for cows fed a diet of 28.1% starch and 4.6% water-soluble carbohydrate compared with diets with lower starch and higher water-soluble carbohydrate contents.
30591340	0	12	theme	dairy	199:203	arg1	cows					205:208	dairy cows	199:208	dairy cows	199:208	Starch and dextrose at 2 levels of rumen-degradable protein in iso-nitrogenous diets: Effects on lactation performance, ruminal measurements, methane emission, digestibility, and nitrogen balance of dairy cows.
30591340	12	13	dep	efficiency	2526:2535	arg1	conversion					2538:2547	conversion	2538:2547	conversion of intake N into milk protein	2538:2577	In this study, treatments did not affect yield (20.0 g of CH4/kg of DMI) or intensity (13.1 g of CH4/kg of fat- and protein-corrected milk), but methane production (g of CH4/d) was 7.0% lower and N use efficiency (conversion of intake N into milk protein) was 7.8% higher for cows fed a diet of 28.1% starch and 4.6% water-soluble carbohydrate compared with diets with lower starch and higher water-soluble carbohydrate contents.
30591340	8	14	theme	molar	1468:1472	arg1	percentage					1474:1483	the molar percentage	1464:1483	the molar percentage of ruminal branched-chain volatile fatty acids	1464:1530	Replacing refined starch with dextrose increased DMI, the molar percentage of ruminal butyrate and valerate, daily CH4 production (g/d), and fecal N and decreased the molar percentage of ruminal branched-chain volatile fatty acids, feed efficiency (fat- and protein-corrected milk/DMI), and N use efficiency (milk N/intake N) but did not influence nutrient digestibility.
30591340	7	15	theme	RDP	1182:1184	arg1	interaction					1192:1202	Carbohydrate source × RDP level interaction	1160:1202	Carbohydrate source × RDP level interaction	1160:1202	Carbohydrate source × RDP level interaction tended to influence dry matter intake (DMI), the concentration of urinary N, and urinary urea-N.
30591340	1	16	from	effects	248:254	arg1	performance					414:424	lactation performance	404:424	lactation performance	404:424	Our objectives were to determine the effects of readily rumen-available carbohydrate source (refined starch vs. dextrose), the level of rumen-degradable protein (RDP), and their interaction on lactation performance, ruminal measurements, enteric methane (CH4) emission, nutrient digestibility, and nitrogen (N) balance in lactating dairy cows.
30591340	1	16	from	effects	248:254	arg1	measurements					435:446	ruminal measurements	427:446	ruminal measurements	427:446	Our objectives were to determine the effects of readily rumen-available carbohydrate source (refined starch vs. dextrose), the level of rumen-degradable protein (RDP), and their interaction on lactation performance, ruminal measurements, enteric methane (CH4) emission, nutrient digestibility, and nitrogen (N) balance in lactating dairy cows.
30591340	1	16	from	effects	248:254	arg1	balance					522:528	nitrogen (N) balance	509:528	nitrogen (N) balance	509:528	Our objectives were to determine the effects of readily rumen-available carbohydrate source (refined starch vs. dextrose), the level of rumen-degradable protein (RDP), and their interaction on lactation performance, ruminal measurements, enteric methane (CH4) emission, nutrient digestibility, and nitrogen (N) balance in lactating dairy cows.
30591340	1	16	from	effects	248:254	arg1	emission					471:478	enteric methane (CH4) emission	449:478	enteric methane (CH4) emission	449:478	Our objectives were to determine the effects of readily rumen-available carbohydrate source (refined starch vs. dextrose), the level of rumen-degradable protein (RDP), and their interaction on lactation performance, ruminal measurements, enteric methane (CH4) emission, nutrient digestibility, and nitrogen (N) balance in lactating dairy cows.
30591340	1	16	from	effects	248:254	arg1	digestibility					490:502	nutrient digestibility	481:502	nutrient digestibility	481:502	Our objectives were to determine the effects of readily rumen-available carbohydrate source (refined starch vs. dextrose), the level of rumen-degradable protein (RDP), and their interaction on lactation performance, ruminal measurements, enteric methane (CH4) emission, nutrient digestibility, and nitrogen (N) balance in lactating dairy cows.
30591340	5	17	theme	starch	926:931	arg1	ratio					942:946	10:0 refined starch:dextrose ratio	913:946	10:0 refined starch:dextrose ratio (% of dietary dry matter)	913:972	In the subplots, the effects of 0:10, 5:5, and 10:0 refined starch:dextrose ratio (% of dietary dry matter) were determined in three 3 × 3 Latin squares by randomly assigning the 9 cows in each RDP level into squares.
30591340	7	18	theme	source	1173:1178	arg1	interaction					1192:1202	Carbohydrate source × RDP level interaction	1160:1202	Carbohydrate source × RDP level interaction	1160:1202	Carbohydrate source × RDP level interaction tended to influence dry matter intake (DMI), the concentration of urinary N, and urinary urea-N.
30591340	9	19	theme	Enteric	1673:1679	arg1	production					1685:1694	Enteric CH4 production	1673:1694	Enteric CH4 production	1673:1694	Enteric CH4 production was negatively related to the molar percentage of ruminal propionate but positively related to the molar percentage of ruminal butyrate.
30591340	0	20	theme	ruminal	120:126	arg1	measurements					128:139	ruminal measurements	120:139	ruminal measurements	120:139	Starch and dextrose at 2 levels of rumen-degradable protein in iso-nitrogenous diets: Effects on lactation performance, ruminal measurements, methane emission, digestibility, and nitrogen balance of dairy cows.
30591340	5	21	theme	dextrose	933:940	arg1	ratio					942:946	10:0 refined starch:dextrose ratio	913:946	10:0 refined starch:dextrose ratio (% of dietary dry matter)	913:972	In the subplots, the effects of 0:10, 5:5, and 10:0 refined starch:dextrose ratio (% of dietary dry matter) were determined in three 3 × 3 Latin squares by randomly assigning the 9 cows in each RDP level into squares.
30591340	8	22	theme	branched-chain	1496:1509	arg1	acids					1526:1530	ruminal branched-chain volatile fatty acids	1488:1530	ruminal branched-chain volatile fatty acids	1488:1530	Replacing refined starch with dextrose increased DMI, the molar percentage of ruminal butyrate and valerate, daily CH4 production (g/d), and fecal N and decreased the molar percentage of ruminal branched-chain volatile fatty acids, feed efficiency (fat- and protein-corrected milk/DMI), and N use efficiency (milk N/intake N) but did not influence nutrient digestibility.
30591340	10	23	theme	9	1902:1902	arg1	%					1903:1903	%	1903:1903	%	1903:1903	Treatments did not influence milk production responses, but cows fed 9% RDP diets had lower ruminal ammonia concentration (7.2 vs. 12.3 mg/dL) and tended to excrete less urinary purine derivatives (428 vs. 493 mmol/d) compared with cows fed 11% RDP diets, suggesting lower ruminal synthesis of microbial protein.
30591340	10	24	contain	had	1915:1917	arg2	mg/dL					1969:1973	7.2 vs. 12.3 mg/dL	1956:1973	7.2 vs. 12.3 mg/dL	1956:1973	Treatments did not influence milk production responses, but cows fed 9% RDP diets had lower ruminal ammonia concentration (7.2 vs. 12.3 mg/dL) and tended to excrete less urinary purine derivatives (428 vs. 493 mmol/d) compared with cows fed 11% RDP diets, suggesting lower ruminal synthesis of microbial protein.
30591340	10	24	contain	had	1915:1917	arg1	cows					1893:1896	cows	1893:1896	cows fed 9% RDP diets	1893:1913	Treatments did not influence milk production responses, but cows fed 9% RDP diets had lower ruminal ammonia concentration (7.2 vs. 12.3 mg/dL) and tended to excrete less urinary purine derivatives (428 vs. 493 mmol/d) compared with cows fed 11% RDP diets, suggesting lower ruminal synthesis of microbial protein.
30591340	10	24	contain	had	1915:1917	arg2	concentration					1941:1953	lower ruminal ammonia concentration	1919:1953	lower ruminal ammonia concentration (7.2 vs. 12.3 mg/dL)	1919:1974	Treatments did not influence milk production responses, but cows fed 9% RDP diets had lower ruminal ammonia concentration (7.2 vs. 12.3 mg/dL) and tended to excrete less urinary purine derivatives (428 vs. 493 mmol/d) compared with cows fed 11% RDP diets, suggesting lower ruminal synthesis of microbial protein.
30591340	8	25	theme	nutrient	1649:1656	arg1	digestibility					1658:1670	nutrient digestibility	1649:1670	nutrient digestibility	1649:1670	Replacing refined starch with dextrose increased DMI, the molar percentage of ruminal butyrate and valerate, daily CH4 production (g/d), and fecal N and decreased the molar percentage of ruminal branched-chain volatile fatty acids, feed efficiency (fat- and protein-corrected milk/DMI), and N use efficiency (milk N/intake N) but did not influence nutrient digestibility.
30591340	8	26	theme	fatty	1520:1524	arg1	acids					1526:1530	ruminal branched-chain volatile fatty acids	1488:1530	ruminal branched-chain volatile fatty acids	1488:1530	Replacing refined starch with dextrose increased DMI, the molar percentage of ruminal butyrate and valerate, daily CH4 production (g/d), and fecal N and decreased the molar percentage of ruminal branched-chain volatile fatty acids, feed efficiency (fat- and protein-corrected milk/DMI), and N use efficiency (milk N/intake N) but did not influence nutrient digestibility.
30591340	12	27	dep	%	2639:2639	arg1	carbohydrate					2655:2666	water-soluble carbohydrate	2641:2666	4.6% water-soluble carbohydrate	2636:2666	In this study, treatments did not affect yield (20.0 g of CH4/kg of DMI) or intensity (13.1 g of CH4/kg of fat- and protein-corrected milk), but methane production (g of CH4/d) was 7.0% lower and N use efficiency (conversion of intake N into milk protein) was 7.8% higher for cows fed a diet of 28.1% starch and 4.6% water-soluble carbohydrate compared with diets with lower starch and higher water-soluble carbohydrate contents.
30591340	1	28	theme	dairy	543:547	arg1	cows					549:552	lactating dairy cows	533:552	lactating dairy cows	533:552	Our objectives were to determine the effects of readily rumen-available carbohydrate source (refined starch vs. dextrose), the level of rumen-degradable protein (RDP), and their interaction on lactation performance, ruminal measurements, enteric methane (CH4) emission, nutrient digestibility, and nitrogen (N) balance in lactating dairy cows.
30591340	5	29	from	cows	1047:1050	arg1	level					1064:1068	each RDP level	1055:1068	each RDP level	1055:1068	In the subplots, the effects of 0:10, 5:5, and 10:0 refined starch:dextrose ratio (% of dietary dry matter) were determined in three 3 × 3 Latin squares by randomly assigning the 9 cows in each RDP level into squares.
30591340	5	30	theme	10:0	913:916	arg1	ratio					942:946	10:0 refined starch:dextrose ratio	913:946	10:0 refined starch:dextrose ratio (% of dietary dry matter)	913:972	In the subplots, the effects of 0:10, 5:5, and 10:0 refined starch:dextrose ratio (% of dietary dry matter) were determined in three 3 × 3 Latin squares by randomly assigning the 9 cows in each RDP level into squares.
30591340	12	31	theme	N	2559:2559	arg1	conversion					2538:2547	conversion	2538:2547	conversion of intake N into milk protein	2538:2577	In this study, treatments did not affect yield (20.0 g of CH4/kg of DMI) or intensity (13.1 g of CH4/kg of fat- and protein-corrected milk), but methane production (g of CH4/d) was 7.0% lower and N use efficiency (conversion of intake N into milk protein) was 7.8% higher for cows fed a diet of 28.1% starch and 4.6% water-soluble carbohydrate compared with diets with lower starch and higher water-soluble carbohydrate contents.
30591340	3	32	theme	soybean	761:767	arg1	meal					792:795	expeller soybean meal	775:795	expeller soybean meal	775:795	The main plots were created by randomly assigning 9 cows to diets of 11 or 9% RDP obtained by altering the percentage of soybean meal, expeller soybean meal, and blood meal in the diet.
30591340	3	32	theme	soybean	761:767	arg1	meal					769:772	soybean meal	761:772	soybean meal	761:772	The main plots were created by randomly assigning 9 cows to diets of 11 or 9% RDP obtained by altering the percentage of soybean meal, expeller soybean meal, and blood meal in the diet.
30591340	10	33	theme	11	2074:2075	arg1	%					2076:2076	%	2076:2076	%	2076:2076	Treatments did not influence milk production responses, but cows fed 9% RDP diets had lower ruminal ammonia concentration (7.2 vs. 12.3 mg/dL) and tended to excrete less urinary purine derivatives (428 vs. 493 mmol/d) compared with cows fed 11% RDP diets, suggesting lower ruminal synthesis of microbial protein.
30591340	11	34	theme	apparent	2223:2230	arg1	digestibility					2244:2256	nutrient apparent total-tract digestibility	2214:2256	nutrient apparent total-tract digestibility	2214:2256	Reducing the level of RDP in iso-nitrogenous diets had no effect on nutrient apparent total-tract digestibility, manure excretion and composition, N balance, and CH4 production.
30591340	0	35	theme	protein	52:58	arg1	levels					25:30	2 levels	23:30	2 levels of rumen-degradable protein	23:58	Starch and dextrose at 2 levels of rumen-degradable protein in iso-nitrogenous diets: Effects on lactation performance, ruminal measurements, methane emission, digestibility, and nitrogen balance of dairy cows.
30591340	5	36	theme	dry	962:964	arg1	matter					966:971	dietary dry matter	954:971	dietary dry matter	954:971	In the subplots, the effects of 0:10, 5:5, and 10:0 refined starch:dextrose ratio (% of dietary dry matter) were determined in three 3 × 3 Latin squares by randomly assigning the 9 cows in each RDP level into squares.
30591340	12	37	theme	milk	2566:2569	arg1	protein					2571:2577	milk protein	2566:2577	milk protein	2566:2577	In this study, treatments did not affect yield (20.0 g of CH4/kg of DMI) or intensity (13.1 g of CH4/kg of fat- and protein-corrected milk), but methane production (g of CH4/d) was 7.0% lower and N use efficiency (conversion of intake N into milk protein) was 7.8% higher for cows fed a diet of 28.1% starch and 4.6% water-soluble carbohydrate compared with diets with lower starch and higher water-soluble carbohydrate contents.
30591340	0	38	from	dextrose	11:18	arg1	diets					79:83	iso-nitrogenous diets	63:83	iso-nitrogenous diets	63:83	Starch and dextrose at 2 levels of rumen-degradable protein in iso-nitrogenous diets: Effects on lactation performance, ruminal measurements, methane emission, digestibility, and nitrogen balance of dairy cows.
30591340	9	39	theme	molar	1726:1730	arg1	percentage					1732:1741	the molar percentage	1722:1741	the molar percentage of ruminal propionate	1722:1763	Enteric CH4 production was negatively related to the molar percentage of ruminal propionate but positively related to the molar percentage of ruminal butyrate.
30591340	0	40	theme	iso-nitrogenous	63:77	arg1	diets					79:83	iso-nitrogenous diets	63:83	iso-nitrogenous diets	63:83	Starch and dextrose at 2 levels of rumen-degradable protein in iso-nitrogenous diets: Effects on lactation performance, ruminal measurements, methane emission, digestibility, and nitrogen balance of dairy cows.
30591340	11	41	theme	nutrient	2214:2221	arg1	digestibility					2244:2256	nutrient apparent total-tract digestibility	2214:2256	nutrient apparent total-tract digestibility	2214:2256	Reducing the level of RDP in iso-nitrogenous diets had no effect on nutrient apparent total-tract digestibility, manure excretion and composition, N balance, and CH4 production.
30591340	3	42	theme	soybean	784:790	arg1	meal					792:795	expeller soybean meal	775:795	expeller soybean meal	775:795	The main plots were created by randomly assigning 9 cows to diets of 11 or 9% RDP obtained by altering the percentage of soybean meal, expeller soybean meal, and blood meal in the diet.
30591340	3	42	theme	soybean	784:790	arg1	meal					769:772	soybean meal	761:772	soybean meal	761:772	The main plots were created by randomly assigning 9 cows to diets of 11 or 9% RDP obtained by altering the percentage of soybean meal, expeller soybean meal, and blood meal in the diet.
30591340	4	43	theme	16.4	845:848	arg1	%					849:849	%	849:849	%	849:849	All diets included 16.4% crude protein.
30591340	0	44	from	Starch	0:5	arg1	diets					79:83	iso-nitrogenous diets	63:83	iso-nitrogenous diets	63:83	Starch and dextrose at 2 levels of rumen-degradable protein in iso-nitrogenous diets: Effects on lactation performance, ruminal measurements, methane emission, digestibility, and nitrogen balance of dairy cows.
30591340	6	45	theme	sample	1141:1146	arg1	collection					1148:1157	sample collection	1141:1157	sample collection	1141:1157	Each period lasted 4 wk, with the last 2 wk allotted for sample collection.
30591340	8	46	theme	protein-corrected	1559:1575	arg1	milk/DMI					1577:1584	protein-corrected milk/DMI	1559:1584	protein-corrected milk/DMI	1559:1584	Replacing refined starch with dextrose increased DMI, the molar percentage of ruminal butyrate and valerate, daily CH4 production (g/d), and fecal N and decreased the molar percentage of ruminal branched-chain volatile fatty acids, feed efficiency (fat- and protein-corrected milk/DMI), and N use efficiency (milk N/intake N) but did not influence nutrient digestibility.
30591340	4	47	theme	crude	851:855	arg1	protein					857:863	16.4% crude protein	845:863	16.4% crude protein	845:863	All diets included 16.4% crude protein.
30591340	9	48	theme	propionate	1754:1763	arg1	percentage					1732:1741	the molar percentage	1722:1741	the molar percentage of ruminal propionate	1722:1763	Enteric CH4 production was negatively related to the molar percentage of ruminal propionate but positively related to the molar percentage of ruminal butyrate.
30591340	3	49	theme	blood	802:806	arg1	meal					808:811	blood meal	802:811	blood meal in the diet	802:823	The main plots were created by randomly assigning 9 cows to diets of 11 or 9% RDP obtained by altering the percentage of soybean meal, expeller soybean meal, and blood meal in the diet.
30591340	1	50	from	digestibility	490:502	arg1	cows					549:552	lactating dairy cows	533:552	lactating dairy cows	533:552	Our objectives were to determine the effects of readily rumen-available carbohydrate source (refined starch vs. dextrose), the level of rumen-degradable protein (RDP), and their interaction on lactation performance, ruminal measurements, enteric methane (CH4) emission, nutrient digestibility, and nitrogen (N) balance in lactating dairy cows.
30591340	12	51	theme	methane	2469:2475	arg1	production					2477:2486	methane production	2469:2486	methane production (g of CH4/d)	2469:2499	In this study, treatments did not affect yield (20.0 g of CH4/kg of DMI) or intensity (13.1 g of CH4/kg of fat- and protein-corrected milk), but methane production (g of CH4/d) was 7.0% lower and N use efficiency (conversion of intake N into milk protein) was 7.8% higher for cows fed a diet of 28.1% starch and 4.6% water-soluble carbohydrate compared with diets with lower starch and higher water-soluble carbohydrate contents.
30591340	12	52	theme	intake	2552:2557	arg1	N					2559:2559	intake N	2552:2559	intake N	2552:2559	In this study, treatments did not affect yield (20.0 g of CH4/kg of DMI) or intensity (13.1 g of CH4/kg of fat- and protein-corrected milk), but methane production (g of CH4/d) was 7.0% lower and N use efficiency (conversion of intake N into milk protein) was 7.8% higher for cows fed a diet of 28.1% starch and 4.6% water-soluble carbohydrate compared with diets with lower starch and higher water-soluble carbohydrate contents.
30591340	1	53	theme	interaction	389:399	arg1	effects					248:254	the effects	244:254	the effects of readily rumen-available carbohydrate source (refined starch vs. dextrose), the level of rumen-degradable protein (RDP), and their interaction on lactation performance, ruminal measurements, enteric methane (CH4) emission, nutrient digestibility, and nitrogen (N) balance in lactating dairy cows	244:552	Our objectives were to determine the effects of readily rumen-available carbohydrate source (refined starch vs. dextrose), the level of rumen-degradable protein (RDP), and their interaction on lactation performance, ruminal measurements, enteric methane (CH4) emission, nutrient digestibility, and nitrogen (N) balance in lactating dairy cows.
30591340	10	54	theme	purine	2011:2016	arg1	mmol/d					2043:2048	428 vs. 493 mmol/d	2031:2048	428 vs. 493 mmol/d	2031:2048	Treatments did not influence milk production responses, but cows fed 9% RDP diets had lower ruminal ammonia concentration (7.2 vs. 12.3 mg/dL) and tended to excrete less urinary purine derivatives (428 vs. 493 mmol/d) compared with cows fed 11% RDP diets, suggesting lower ruminal synthesis of microbial protein.
30591340	10	54	theme	purine	2011:2016	arg1	derivatives					2018:2028	less urinary purine derivatives	1998:2028	less urinary purine derivatives (428 vs. 493 mmol/d)	1998:2049	Treatments did not influence milk production responses, but cows fed 9% RDP diets had lower ruminal ammonia concentration (7.2 vs. 12.3 mg/dL) and tended to excrete less urinary purine derivatives (428 vs. 493 mmol/d) compared with cows fed 11% RDP diets, suggesting lower ruminal synthesis of microbial protein.
30591340	12	55	theme	starch	2699:2704	arg1	contents					2744:2751	lower starch and higher water-soluble carbohydrate contents	2693:2751	lower starch and higher water-soluble carbohydrate contents	2693:2751	In this study, treatments did not affect yield (20.0 g of CH4/kg of DMI) or intensity (13.1 g of CH4/kg of fat- and protein-corrected milk), but methane production (g of CH4/d) was 7.0% lower and N use efficiency (conversion of intake N into milk protein) was 7.8% higher for cows fed a diet of 28.1% starch and 4.6% water-soluble carbohydrate compared with diets with lower starch and higher water-soluble carbohydrate contents.
30591340	10	56	theme	lower	2100:2104	arg1	synthesis					2114:2122	lower ruminal synthesis	2100:2122	lower ruminal synthesis of microbial protein	2100:2143	Treatments did not influence milk production responses, but cows fed 9% RDP diets had lower ruminal ammonia concentration (7.2 vs. 12.3 mg/dL) and tended to excrete less urinary purine derivatives (428 vs. 493 mmol/d) compared with cows fed 11% RDP diets, suggesting lower ruminal synthesis of microbial protein.
30591340	8	57	theme	refined	1311:1317	arg1	starch					1319:1324	refined starch	1311:1324	refined starch	1311:1324	Replacing refined starch with dextrose increased DMI, the molar percentage of ruminal butyrate and valerate, daily CH4 production (g/d), and fecal N and decreased the molar percentage of ruminal branched-chain volatile fatty acids, feed efficiency (fat- and protein-corrected milk/DMI), and N use efficiency (milk N/intake N) but did not influence nutrient digestibility.
30591340	11	58	theme	RDP	2168:2170	arg1	level					2159:2163	the level	2155:2163	the level of RDP	2155:2170	Reducing the level of RDP in iso-nitrogenous diets had no effect on nutrient apparent total-tract digestibility, manure excretion and composition, N balance, and CH4 production.
30591340	12	59	theme	N	2520:2520	arg1	efficiency					2526:2535	N use efficiency	2520:2535	N use efficiency (conversion of intake N into milk protein)	2520:2578	In this study, treatments did not affect yield (20.0 g of CH4/kg of DMI) or intensity (13.1 g of CH4/kg of fat- and protein-corrected milk), but methane production (g of CH4/d) was 7.0% lower and N use efficiency (conversion of intake N into milk protein) was 7.8% higher for cows fed a diet of 28.1% starch and 4.6% water-soluble carbohydrate compared with diets with lower starch and higher water-soluble carbohydrate contents.
30591340	1	60	theme	rumen-available	267:281	arg1	source					296:301	readily rumen-available carbohydrate source	259:301	readily rumen-available carbohydrate source (refined starch vs. dextrose)	259:331	Our objectives were to determine the effects of readily rumen-available carbohydrate source (refined starch vs. dextrose), the level of rumen-degradable protein (RDP), and their interaction on lactation performance, ruminal measurements, enteric methane (CH4) emission, nutrient digestibility, and nitrogen (N) balance in lactating dairy cows.
30591340	1	61	theme	protein	364:370	arg1	level					338:342	the level	334:342	the level of rumen-degradable protein (RDP)	334:376	Our objectives were to determine the effects of readily rumen-available carbohydrate source (refined starch vs. dextrose), the level of rumen-degradable protein (RDP), and their interaction on lactation performance, ruminal measurements, enteric methane (CH4) emission, nutrient digestibility, and nitrogen (N) balance in lactating dairy cows.
30591340	1	61	theme	protein	364:370	arg1	source					296:301	readily rumen-available carbohydrate source	259:301	readily rumen-available carbohydrate source (refined starch vs. dextrose)	259:331	Our objectives were to determine the effects of readily rumen-available carbohydrate source (refined starch vs. dextrose), the level of rumen-degradable protein (RDP), and their interaction on lactation performance, ruminal measurements, enteric methane (CH4) emission, nutrient digestibility, and nitrogen (N) balance in lactating dairy cows.
30591340	1	61	theme	protein	364:370	arg1	interaction					389:399	their interaction	383:399	their interaction	383:399	Our objectives were to determine the effects of readily rumen-available carbohydrate source (refined starch vs. dextrose), the level of rumen-degradable protein (RDP), and their interaction on lactation performance, ruminal measurements, enteric methane (CH4) emission, nutrient digestibility, and nitrogen (N) balance in lactating dairy cows.
30591340	3	62	from	percentage	747:756	arg1	diet					820:823	the diet	816:823	the diet	816:823	The main plots were created by randomly assigning 9 cows to diets of 11 or 9% RDP obtained by altering the percentage of soybean meal, expeller soybean meal, and blood meal in the diet.
30591340	9	63	theme	molar	1795:1799	arg1	percentage					1801:1810	the molar percentage	1791:1810	the molar percentage of ruminal butyrate	1791:1830	Enteric CH4 production was negatively related to the molar percentage of ruminal propionate but positively related to the molar percentage of ruminal butyrate.
30591340	1	64	theme	source	296:301	arg1	effects					248:254	the effects	244:254	the effects of readily rumen-available carbohydrate source (refined starch vs. dextrose), the level of rumen-degradable protein (RDP), and their interaction on lactation performance, ruminal measurements, enteric methane (CH4) emission, nutrient digestibility, and nitrogen (N) balance in lactating dairy cows	244:552	Our objectives were to determine the effects of readily rumen-available carbohydrate source (refined starch vs. dextrose), the level of rumen-degradable protein (RDP), and their interaction on lactation performance, ruminal measurements, enteric methane (CH4) emission, nutrient digestibility, and nitrogen (N) balance in lactating dairy cows.
30591340	7	65	theme	urinary	1285:1291	arg1	urea-N					1293:1298	urinary urea-N	1285:1298	urinary urea-N	1285:1298	Carbohydrate source × RDP level interaction tended to influence dry matter intake (DMI), the concentration of urinary N, and urinary urea-N.
30591340	5	66	theme	0:10	898:901	arg1	effects					887:893	the effects	883:893	the effects of 0:10, 5:5, and 10:0 refined starch:dextrose ratio (% of dietary dry matter)	883:972	In the subplots, the effects of 0:10, 5:5, and 10:0 refined starch:dextrose ratio (% of dietary dry matter) were determined in three 3 × 3 Latin squares by randomly assigning the 9 cows in each RDP level into squares.
30591340	10	67	theme	microbial	2127:2135	arg1	protein					2137:2143	microbial protein	2127:2143	microbial protein	2127:2143	Treatments did not influence milk production responses, but cows fed 9% RDP diets had lower ruminal ammonia concentration (7.2 vs. 12.3 mg/dL) and tended to excrete less urinary purine derivatives (428 vs. 493 mmol/d) compared with cows fed 11% RDP diets, suggesting lower ruminal synthesis of microbial protein.
30591340	12	68	theme	higher	2710:2715	arg1	contents					2744:2751	lower starch and higher water-soluble carbohydrate contents	2693:2751	lower starch and higher water-soluble carbohydrate contents	2693:2751	In this study, treatments did not affect yield (20.0 g of CH4/kg of DMI) or intensity (13.1 g of CH4/kg of fat- and protein-corrected milk), but methane production (g of CH4/d) was 7.0% lower and N use efficiency (conversion of intake N into milk protein) was 7.8% higher for cows fed a diet of 28.1% starch and 4.6% water-soluble carbohydrate compared with diets with lower starch and higher water-soluble carbohydrate contents.
30591340	1	69	theme	refined	304:310	arg1	starch					312:317	refined starch	304:317	refined starch	304:317	Our objectives were to determine the effects of readily rumen-available carbohydrate source (refined starch vs. dextrose), the level of rumen-degradable protein (RDP), and their interaction on lactation performance, ruminal measurements, enteric methane (CH4) emission, nutrient digestibility, and nitrogen (N) balance in lactating dairy cows.
30591340	5	70	theme	5:5	904:906	arg1	effects					887:893	the effects	883:893	the effects of 0:10, 5:5, and 10:0 refined starch:dextrose ratio (% of dietary dry matter)	883:972	In the subplots, the effects of 0:10, 5:5, and 10:0 refined starch:dextrose ratio (% of dietary dry matter) were determined in three 3 × 3 Latin squares by randomly assigning the 9 cows in each RDP level into squares.
30591340	8	71	dep	efficiency	1538:1547	arg1	fat-					1550:1553	fat-	1550:1553	fat-	1550:1553	Replacing refined starch with dextrose increased DMI, the molar percentage of ruminal butyrate and valerate, daily CH4 production (g/d), and fecal N and decreased the molar percentage of ruminal branched-chain volatile fatty acids, feed efficiency (fat- and protein-corrected milk/DMI), and N use efficiency (milk N/intake N) but did not influence nutrient digestibility.
30591340	8	71	dep	efficiency	1538:1547	arg1	milk/DMI					1577:1584	protein-corrected milk/DMI	1559:1584	protein-corrected milk/DMI	1559:1584	Replacing refined starch with dextrose increased DMI, the molar percentage of ruminal butyrate and valerate, daily CH4 production (g/d), and fecal N and decreased the molar percentage of ruminal branched-chain volatile fatty acids, feed efficiency (fat- and protein-corrected milk/DMI), and N use efficiency (milk N/intake N) but did not influence nutrient digestibility.
30591340	9	72	theme	butyrate	1823:1830	arg1	percentage					1801:1810	the molar percentage	1791:1810	the molar percentage of ruminal butyrate	1791:1830	Enteric CH4 production was negatively related to the molar percentage of ruminal propionate but positively related to the molar percentage of ruminal butyrate.
30591340	8	73	theme	daily	1410:1414	arg1	production					1420:1429	daily CH4 production	1410:1429	daily CH4 production (g/d)	1410:1435	Replacing refined starch with dextrose increased DMI, the molar percentage of ruminal butyrate and valerate, daily CH4 production (g/d), and fecal N and decreased the molar percentage of ruminal branched-chain volatile fatty acids, feed efficiency (fat- and protein-corrected milk/DMI), and N use efficiency (milk N/intake N) but did not influence nutrient digestibility.
30591340	8	73	theme	daily	1410:1414	arg1	g/d					1432:1434	g/d	1432:1434	g/d	1432:1434	Replacing refined starch with dextrose increased DMI, the molar percentage of ruminal butyrate and valerate, daily CH4 production (g/d), and fecal N and decreased the molar percentage of ruminal branched-chain volatile fatty acids, feed efficiency (fat- and protein-corrected milk/DMI), and N use efficiency (milk N/intake N) but did not influence nutrient digestibility.
30591340	7	74	theme	urinary	1270:1276	arg1	N					1278:1278	urinary N	1270:1278	urinary N	1270:1278	Carbohydrate source × RDP level interaction tended to influence dry matter intake (DMI), the concentration of urinary N, and urinary urea-N.
30591340	1	75	from	measurements	435:446	arg1	cows					549:552	lactating dairy cows	533:552	lactating dairy cows	533:552	Our objectives were to determine the effects of readily rumen-available carbohydrate source (refined starch vs. dextrose), the level of rumen-degradable protein (RDP), and their interaction on lactation performance, ruminal measurements, enteric methane (CH4) emission, nutrient digestibility, and nitrogen (N) balance in lactating dairy cows.
30591340	5	76	dep	ratio	942:946	arg1	matter					966:971	dietary dry matter	954:971	dietary dry matter	954:971	In the subplots, the effects of 0:10, 5:5, and 10:0 refined starch:dextrose ratio (% of dietary dry matter) were determined in three 3 × 3 Latin squares by randomly assigning the 9 cows in each RDP level into squares.
30591340	5	76	dep	ratio	942:946	arg1	%					949:949	%	949:949	% of dietary dry matter	949:971	In the subplots, the effects of 0:10, 5:5, and 10:0 refined starch:dextrose ratio (% of dietary dry matter) were determined in three 3 × 3 Latin squares by randomly assigning the 9 cows in each RDP level into squares.
30591340	12	77	theme	use	2522:2524	arg1	efficiency					2526:2535	N use efficiency	2520:2535	N use efficiency (conversion of intake N into milk protein)	2520:2578	In this study, treatments did not affect yield (20.0 g of CH4/kg of DMI) or intensity (13.1 g of CH4/kg of fat- and protein-corrected milk), but methane production (g of CH4/d) was 7.0% lower and N use efficiency (conversion of intake N into milk protein) was 7.8% higher for cows fed a diet of 28.1% starch and 4.6% water-soluble carbohydrate compared with diets with lower starch and higher water-soluble carbohydrate contents.
30591340	0	78	dep	Starch	0:5	arg1	Effects					86:92	Effects	86:92	Starch and dextrose at 2 levels of rumen-degradable protein in iso-nitrogenous diets: Effects on lactation performance, ruminal measurements, methane emission, digestibility, and nitrogen balance of dairy cows.	0:209	Starch and dextrose at 2 levels of rumen-degradable protein in iso-nitrogenous diets: Effects on lactation performance, ruminal measurements, methane emission, digestibility, and nitrogen balance of dairy cows.
30591340	11	79	theme	manure	2259:2264	arg1	excretion					2266:2274	manure excretion	2259:2274	manure excretion	2259:2274	Reducing the level of RDP in iso-nitrogenous diets had no effect on nutrient apparent total-tract digestibility, manure excretion and composition, N balance, and CH4 production.
30591340	8	80	theme	use	1594:1596	arg1	efficiency					1598:1607	N use efficiency	1592:1607	N use efficiency (milk N/intake N)	1592:1625	Replacing refined starch with dextrose increased DMI, the molar percentage of ruminal butyrate and valerate, daily CH4 production (g/d), and fecal N and decreased the molar percentage of ruminal branched-chain volatile fatty acids, feed efficiency (fat- and protein-corrected milk/DMI), and N use efficiency (milk N/intake N) but did not influence nutrient digestibility.
30591340	8	80	theme	use	1594:1596	arg1	N					1624:1624	milk N/intake N	1610:1624	milk N/intake N	1610:1624	Replacing refined starch with dextrose increased DMI, the molar percentage of ruminal butyrate and valerate, daily CH4 production (g/d), and fecal N and decreased the molar percentage of ruminal branched-chain volatile fatty acids, feed efficiency (fat- and protein-corrected milk/DMI), and N use efficiency (milk N/intake N) but did not influence nutrient digestibility.
30591340	2	81	used	used	609:612	arg2	cows					599:602	Eighteen mid-lactation multiparous Holstein cows	555:602	Eighteen mid-lactation multiparous Holstein cows	555:602	Eighteen mid-lactation multiparous Holstein cows were used in this split-plot study.
30591340	12	82	theme	DMI	2392:2394	arg1	CH4/kg					2382:2387	CH4/kg	2382:2387	CH4/kg of DMI	2382:2394	In this study, treatments did not affect yield (20.0 g of CH4/kg of DMI) or intensity (13.1 g of CH4/kg of fat- and protein-corrected milk), but methane production (g of CH4/d) was 7.0% lower and N use efficiency (conversion of intake N into milk protein) was 7.8% higher for cows fed a diet of 28.1% starch and 4.6% water-soluble carbohydrate compared with diets with lower starch and higher water-soluble carbohydrate contents.
30591340	8	83	theme	butyrate	1387:1394	arg1	production					1420:1429	daily CH4 production	1410:1429	daily CH4 production (g/d)	1410:1435	Replacing refined starch with dextrose increased DMI, the molar percentage of ruminal butyrate and valerate, daily CH4 production (g/d), and fecal N and decreased the molar percentage of ruminal branched-chain volatile fatty acids, feed efficiency (fat- and protein-corrected milk/DMI), and N use efficiency (milk N/intake N) but did not influence nutrient digestibility.
30591340	8	83	theme	butyrate	1387:1394	arg1	DMI					1350:1352	DMI	1350:1352	DMI	1350:1352	Replacing refined starch with dextrose increased DMI, the molar percentage of ruminal butyrate and valerate, daily CH4 production (g/d), and fecal N and decreased the molar percentage of ruminal branched-chain volatile fatty acids, feed efficiency (fat- and protein-corrected milk/DMI), and N use efficiency (milk N/intake N) but did not influence nutrient digestibility.
30591340	8	83	theme	butyrate	1387:1394	arg1	N					1448:1448	fecal N	1442:1448	fecal N	1442:1448	Replacing refined starch with dextrose increased DMI, the molar percentage of ruminal butyrate and valerate, daily CH4 production (g/d), and fecal N and decreased the molar percentage of ruminal branched-chain volatile fatty acids, feed efficiency (fat- and protein-corrected milk/DMI), and N use efficiency (milk N/intake N) but did not influence nutrient digestibility.
30591340	8	83	theme	butyrate	1387:1394	arg1	g/d					1432:1434	g/d	1432:1434	g/d	1432:1434	Replacing refined starch with dextrose increased DMI, the molar percentage of ruminal butyrate and valerate, daily CH4 production (g/d), and fecal N and decreased the molar percentage of ruminal branched-chain volatile fatty acids, feed efficiency (fat- and protein-corrected milk/DMI), and N use efficiency (milk N/intake N) but did not influence nutrient digestibility.
30591340	8	83	theme	butyrate	1387:1394	arg1	percentage					1365:1374	the molar percentage	1355:1374	the molar percentage of ruminal butyrate and valerate	1355:1407	Replacing refined starch with dextrose increased DMI, the molar percentage of ruminal butyrate and valerate, daily CH4 production (g/d), and fecal N and decreased the molar percentage of ruminal branched-chain volatile fatty acids, feed efficiency (fat- and protein-corrected milk/DMI), and N use efficiency (milk N/intake N) but did not influence nutrient digestibility.
30591340	3	84	theme	main	644:647	arg1	plots					649:653	The main plots	640:653	The main plots	640:653	The main plots were created by randomly assigning 9 cows to diets of 11 or 9% RDP obtained by altering the percentage of soybean meal, expeller soybean meal, and blood meal in the diet.
30591340	0	85	theme	nitrogen	179:186	arg1	balance					188:194	nitrogen balance	179:194	nitrogen balance	179:194	Starch and dextrose at 2 levels of rumen-degradable protein in iso-nitrogenous diets: Effects on lactation performance, ruminal measurements, methane emission, digestibility, and nitrogen balance of dairy cows.
30591340	1	86	theme	lactation	404:412	arg1	performance					414:424	lactation performance	404:424	lactation performance	404:424	Our objectives were to determine the effects of readily rumen-available carbohydrate source (refined starch vs. dextrose), the level of rumen-degradable protein (RDP), and their interaction on lactation performance, ruminal measurements, enteric methane (CH4) emission, nutrient digestibility, and nitrogen (N) balance in lactating dairy cows.
30591340	10	87	theme	RDP	1905:1907	arg1	diets					1909:1913	9% RDP diets	1902:1913	9% RDP diets	1902:1913	Treatments did not influence milk production responses, but cows fed 9% RDP diets had lower ruminal ammonia concentration (7.2 vs. 12.3 mg/dL) and tended to excrete less urinary purine derivatives (428 vs. 493 mmol/d) compared with cows fed 11% RDP diets, suggesting lower ruminal synthesis of microbial protein.
30591340	7	88	theme	matter	1228:1233	arg1	DMI					1243:1245	DMI	1243:1245	DMI	1243:1245	Carbohydrate source × RDP level interaction tended to influence dry matter intake (DMI), the concentration of urinary N, and urinary urea-N.
30591340	7	88	theme	matter	1228:1233	arg1	intake					1235:1240	dry matter intake	1224:1240	dry matter intake (DMI)	1224:1246	Carbohydrate source × RDP level interaction tended to influence dry matter intake (DMI), the concentration of urinary N, and urinary urea-N.
30591340	10	89	theme	milk	1862:1865	arg1	production					1867:1876	milk production	1862:1876	milk production responses	1862:1886	Treatments did not influence milk production responses, but cows fed 9% RDP diets had lower ruminal ammonia concentration (7.2 vs. 12.3 mg/dL) and tended to excrete less urinary purine derivatives (428 vs. 493 mmol/d) compared with cows fed 11% RDP diets, suggesting lower ruminal synthesis of microbial protein.
30591340	8	90	theme	valerate	1400:1407	arg1	production					1420:1429	daily CH4 production	1410:1429	daily CH4 production (g/d)	1410:1435	Replacing refined starch with dextrose increased DMI, the molar percentage of ruminal butyrate and valerate, daily CH4 production (g/d), and fecal N and decreased the molar percentage of ruminal branched-chain volatile fatty acids, feed efficiency (fat- and protein-corrected milk/DMI), and N use efficiency (milk N/intake N) but did not influence nutrient digestibility.
30591340	8	90	theme	valerate	1400:1407	arg1	DMI					1350:1352	DMI	1350:1352	DMI	1350:1352	Replacing refined starch with dextrose increased DMI, the molar percentage of ruminal butyrate and valerate, daily CH4 production (g/d), and fecal N and decreased the molar percentage of ruminal branched-chain volatile fatty acids, feed efficiency (fat- and protein-corrected milk/DMI), and N use efficiency (milk N/intake N) but did not influence nutrient digestibility.
30591340	8	90	theme	valerate	1400:1407	arg1	N					1448:1448	fecal N	1442:1448	fecal N	1442:1448	Replacing refined starch with dextrose increased DMI, the molar percentage of ruminal butyrate and valerate, daily CH4 production (g/d), and fecal N and decreased the molar percentage of ruminal branched-chain volatile fatty acids, feed efficiency (fat- and protein-corrected milk/DMI), and N use efficiency (milk N/intake N) but did not influence nutrient digestibility.
30591340	8	90	theme	valerate	1400:1407	arg1	g/d					1432:1434	g/d	1432:1434	g/d	1432:1434	Replacing refined starch with dextrose increased DMI, the molar percentage of ruminal butyrate and valerate, daily CH4 production (g/d), and fecal N and decreased the molar percentage of ruminal branched-chain volatile fatty acids, feed efficiency (fat- and protein-corrected milk/DMI), and N use efficiency (milk N/intake N) but did not influence nutrient digestibility.
30591340	8	90	theme	valerate	1400:1407	arg1	percentage					1365:1374	the molar percentage	1355:1374	the molar percentage of ruminal butyrate and valerate	1355:1407	Replacing refined starch with dextrose increased DMI, the molar percentage of ruminal butyrate and valerate, daily CH4 production (g/d), and fecal N and decreased the molar percentage of ruminal branched-chain volatile fatty acids, feed efficiency (fat- and protein-corrected milk/DMI), and N use efficiency (milk N/intake N) but did not influence nutrient digestibility.
30591340	12	91	theme	%	2623:2623	arg1	starch					2625:2630	28.1% starch	2619:2630	28.1% starch	2619:2630	In this study, treatments did not affect yield (20.0 g of CH4/kg of DMI) or intensity (13.1 g of CH4/kg of fat- and protein-corrected milk), but methane production (g of CH4/d) was 7.0% lower and N use efficiency (conversion of intake N into milk protein) was 7.8% higher for cows fed a diet of 28.1% starch and 4.6% water-soluble carbohydrate compared with diets with lower starch and higher water-soluble carbohydrate contents.
30591340	8	92	theme	N/intake	1615:1622	arg1	efficiency					1598:1607	N use efficiency	1592:1607	N use efficiency (milk N/intake N)	1592:1625	Replacing refined starch with dextrose increased DMI, the molar percentage of ruminal butyrate and valerate, daily CH4 production (g/d), and fecal N and decreased the molar percentage of ruminal branched-chain volatile fatty acids, feed efficiency (fat- and protein-corrected milk/DMI), and N use efficiency (milk N/intake N) but did not influence nutrient digestibility.
30591340	8	92	theme	N/intake	1615:1622	arg1	N					1624:1624	milk N/intake N	1610:1624	milk N/intake N	1610:1624	Replacing refined starch with dextrose increased DMI, the molar percentage of ruminal butyrate and valerate, daily CH4 production (g/d), and fecal N and decreased the molar percentage of ruminal branched-chain volatile fatty acids, feed efficiency (fat- and protein-corrected milk/DMI), and N use efficiency (milk N/intake N) but did not influence nutrient digestibility.
30591340	8	93	theme	fecal	1442:1446	arg1	N					1448:1448	fecal N	1442:1448	fecal N	1442:1448	Replacing refined starch with dextrose increased DMI, the molar percentage of ruminal butyrate and valerate, daily CH4 production (g/d), and fecal N and decreased the molar percentage of ruminal branched-chain volatile fatty acids, feed efficiency (fat- and protein-corrected milk/DMI), and N use efficiency (milk N/intake N) but did not influence nutrient digestibility.
30591340	2	94	theme	multiparous	578:588	arg1	cows					599:602	Eighteen mid-lactation multiparous Holstein cows	555:602	Eighteen mid-lactation multiparous Holstein cows	555:602	Eighteen mid-lactation multiparous Holstein cows were used in this split-plot study.
30591340	12	95	dep	yield	2365:2369	arg1	g					2377:2377	20.0 g	2372:2377	20.0 g of CH4/kg of DMI	2372:2394	In this study, treatments did not affect yield (20.0 g of CH4/kg of DMI) or intensity (13.1 g of CH4/kg of fat- and protein-corrected milk), but methane production (g of CH4/d) was 7.0% lower and N use efficiency (conversion of intake N into milk protein) was 7.8% higher for cows fed a diet of 28.1% starch and 4.6% water-soluble carbohydrate compared with diets with lower starch and higher water-soluble carbohydrate contents.
30591340	0	96	theme	cows	205:208	arg1	Effects					86:92	Effects	86:92	Starch and dextrose at 2 levels of rumen-degradable protein in iso-nitrogenous diets: Effects on lactation performance, ruminal measurements, methane emission, digestibility, and nitrogen balance of dairy cows.	0:209	Starch and dextrose at 2 levels of rumen-degradable protein in iso-nitrogenous diets: Effects on lactation performance, ruminal measurements, methane emission, digestibility, and nitrogen balance of dairy cows.
30591340	12	97	theme	CH4/kg	2382:2387	arg1	g					2377:2377	20.0 g	2372:2377	20.0 g of CH4/kg of DMI	2372:2394	In this study, treatments did not affect yield (20.0 g of CH4/kg of DMI) or intensity (13.1 g of CH4/kg of fat- and protein-corrected milk), but methane production (g of CH4/d) was 7.0% lower and N use efficiency (conversion of intake N into milk protein) was 7.8% higher for cows fed a diet of 28.1% starch and 4.6% water-soluble carbohydrate compared with diets with lower starch and higher water-soluble carbohydrate contents.
30591340	1	98	theme	enteric	449:455	arg1	emission					471:478	enteric methane (CH4) emission	449:478	enteric methane (CH4) emission	449:478	Our objectives were to determine the effects of readily rumen-available carbohydrate source (refined starch vs. dextrose), the level of rumen-degradable protein (RDP), and their interaction on lactation performance, ruminal measurements, enteric methane (CH4) emission, nutrient digestibility, and nitrogen (N) balance in lactating dairy cows.
30591340	5	99	theme	Latin	1005:1009	arg1	squares					1011:1017	three 3 × 3 Latin squares	993:1017	three 3 × 3 Latin squares	993:1017	In the subplots, the effects of 0:10, 5:5, and 10:0 refined starch:dextrose ratio (% of dietary dry matter) were determined in three 3 × 3 Latin squares by randomly assigning the 9 cows in each RDP level into squares.
30591340	7	100	theme	level	1186:1190	arg1	interaction					1192:1202	Carbohydrate source × RDP level interaction	1160:1202	Carbohydrate source × RDP level interaction	1160:1202	Carbohydrate source × RDP level interaction tended to influence dry matter intake (DMI), the concentration of urinary N, and urinary urea-N.
30591340	6	101	theme	last	1118:1121	arg1	wk					1125:1126	the last 2 wk	1114:1126	the last 2 wk allotted for sample collection	1114:1157	Each period lasted 4 wk, with the last 2 wk allotted for sample collection.
30591340	3	102	dep	RDP	718:720	arg1	%					716:716	%	716:716	%	716:716	The main plots were created by randomly assigning 9 cows to diets of 11 or 9% RDP obtained by altering the percentage of soybean meal, expeller soybean meal, and blood meal in the diet.
30591340	10	103	theme	urinary	2003:2009	arg1	mmol/d					2043:2048	428 vs. 493 mmol/d	2031:2048	428 vs. 493 mmol/d	2031:2048	Treatments did not influence milk production responses, but cows fed 9% RDP diets had lower ruminal ammonia concentration (7.2 vs. 12.3 mg/dL) and tended to excrete less urinary purine derivatives (428 vs. 493 mmol/d) compared with cows fed 11% RDP diets, suggesting lower ruminal synthesis of microbial protein.
30591340	10	103	theme	urinary	2003:2009	arg1	derivatives					2018:2028	less urinary purine derivatives	1998:2028	less urinary purine derivatives (428 vs. 493 mmol/d)	1998:2049	Treatments did not influence milk production responses, but cows fed 9% RDP diets had lower ruminal ammonia concentration (7.2 vs. 12.3 mg/dL) and tended to excrete less urinary purine derivatives (428 vs. 493 mmol/d) compared with cows fed 11% RDP diets, suggesting lower ruminal synthesis of microbial protein.
30591340	12	104	theme	carbohydrate	2731:2742	arg1	contents					2744:2751	lower starch and higher water-soluble carbohydrate contents	2693:2751	lower starch and higher water-soluble carbohydrate contents	2693:2751	In this study, treatments did not affect yield (20.0 g of CH4/kg of DMI) or intensity (13.1 g of CH4/kg of fat- and protein-corrected milk), but methane production (g of CH4/d) was 7.0% lower and N use efficiency (conversion of intake N into milk protein) was 7.8% higher for cows fed a diet of 28.1% starch and 4.6% water-soluble carbohydrate compared with diets with lower starch and higher water-soluble carbohydrate contents.
30591340	9	105	theme	CH4	1681:1683	arg1	production					1685:1694	Enteric CH4 production	1673:1694	Enteric CH4 production	1673:1694	Enteric CH4 production was negatively related to the molar percentage of ruminal propionate but positively related to the molar percentage of ruminal butyrate.
30591340	12	106	theme	milk	2458:2461	arg1	CH4/kg					2421:2426	CH4/kg	2421:2426	CH4/kg of fat- and protein-corrected milk	2421:2461	In this study, treatments did not affect yield (20.0 g of CH4/kg of DMI) or intensity (13.1 g of CH4/kg of fat- and protein-corrected milk), but methane production (g of CH4/d) was 7.0% lower and N use efficiency (conversion of intake N into milk protein) was 7.8% higher for cows fed a diet of 28.1% starch and 4.6% water-soluble carbohydrate compared with diets with lower starch and higher water-soluble carbohydrate contents.
30591340	0	107	theme	methane	142:148	arg1	emission					150:157	methane emission	142:157	methane emission	142:157	Starch and dextrose at 2 levels of rumen-degradable protein in iso-nitrogenous diets: Effects on lactation performance, ruminal measurements, methane emission, digestibility, and nitrogen balance of dairy cows.
30591340	5	108	theme	ratio	942:946	arg1	effects					887:893	the effects	883:893	the effects of 0:10, 5:5, and 10:0 refined starch:dextrose ratio (% of dietary dry matter)	883:972	In the subplots, the effects of 0:10, 5:5, and 10:0 refined starch:dextrose ratio (% of dietary dry matter) were determined in three 3 × 3 Latin squares by randomly assigning the 9 cows in each RDP level into squares.
30591340	8	109	theme	ruminal	1488:1494	arg1	acids					1526:1530	ruminal branched-chain volatile fatty acids	1488:1530	ruminal branched-chain volatile fatty acids	1488:1530	Replacing refined starch with dextrose increased DMI, the molar percentage of ruminal butyrate and valerate, daily CH4 production (g/d), and fecal N and decreased the molar percentage of ruminal branched-chain volatile fatty acids, feed efficiency (fat- and protein-corrected milk/DMI), and N use efficiency (milk N/intake N) but did not influence nutrient digestibility.
30591340	11	110	theme	total-tract	2232:2242	arg1	digestibility					2244:2256	nutrient apparent total-tract digestibility	2214:2256	nutrient apparent total-tract digestibility	2214:2256	Reducing the level of RDP in iso-nitrogenous diets had no effect on nutrient apparent total-tract digestibility, manure excretion and composition, N balance, and CH4 production.
30591340	7	111	theme	Carbohydrate	1160:1171	arg1	interaction					1192:1202	Carbohydrate source × RDP level interaction	1160:1202	Carbohydrate source × RDP level interaction	1160:1202	Carbohydrate source × RDP level interaction tended to influence dry matter intake (DMI), the concentration of urinary N, and urinary urea-N.
30591340	1	112	theme	lactating	533:541	arg1	cows					549:552	lactating dairy cows	533:552	lactating dairy cows	533:552	Our objectives were to determine the effects of readily rumen-available carbohydrate source (refined starch vs. dextrose), the level of rumen-degradable protein (RDP), and their interaction on lactation performance, ruminal measurements, enteric methane (CH4) emission, nutrient digestibility, and nitrogen (N) balance in lactating dairy cows.
30591340	8	113	theme	volatile	1511:1518	arg1	acids					1526:1530	ruminal branched-chain volatile fatty acids	1488:1530	ruminal branched-chain volatile fatty acids	1488:1530	Replacing refined starch with dextrose increased DMI, the molar percentage of ruminal butyrate and valerate, daily CH4 production (g/d), and fecal N and decreased the molar percentage of ruminal branched-chain volatile fatty acids, feed efficiency (fat- and protein-corrected milk/DMI), and N use efficiency (milk N/intake N) but did not influence nutrient digestibility.
30591340	3	114	theme	expeller	775:782	arg1	meal					792:795	expeller soybean meal	775:795	expeller soybean meal	775:795	The main plots were created by randomly assigning 9 cows to diets of 11 or 9% RDP obtained by altering the percentage of soybean meal, expeller soybean meal, and blood meal in the diet.
30591340	3	114	theme	expeller	775:782	arg1	meal					769:772	soybean meal	761:772	soybean meal	761:772	The main plots were created by randomly assigning 9 cows to diets of 11 or 9% RDP obtained by altering the percentage of soybean meal, expeller soybean meal, and blood meal in the diet.
30591340	0	115	from	Effects	86:92	arg1	performance					107:117	lactation performance	97:117	lactation performance	97:117	Starch and dextrose at 2 levels of rumen-degradable protein in iso-nitrogenous diets: Effects on lactation performance, ruminal measurements, methane emission, digestibility, and nitrogen balance of dairy cows.
30591340	0	115	from	Effects	86:92	arg1	measurements					128:139	ruminal measurements	120:139	ruminal measurements	120:139	Starch and dextrose at 2 levels of rumen-degradable protein in iso-nitrogenous diets: Effects on lactation performance, ruminal measurements, methane emission, digestibility, and nitrogen balance of dairy cows.
30591340	0	115	from	Effects	86:92	arg1	emission					150:157	methane emission	142:157	methane emission	142:157	Starch and dextrose at 2 levels of rumen-degradable protein in iso-nitrogenous diets: Effects on lactation performance, ruminal measurements, methane emission, digestibility, and nitrogen balance of dairy cows.
30591340	0	115	from	Effects	86:92	arg1	digestibility					160:172	digestibility	160:172	digestibility	160:172	Starch and dextrose at 2 levels of rumen-degradable protein in iso-nitrogenous diets: Effects on lactation performance, ruminal measurements, methane emission, digestibility, and nitrogen balance of dairy cows.
30591340	0	115	from	Effects	86:92	arg1	balance					188:194	nitrogen balance	179:194	nitrogen balance	179:194	Starch and dextrose at 2 levels of rumen-degradable protein in iso-nitrogenous diets: Effects on lactation performance, ruminal measurements, methane emission, digestibility, and nitrogen balance of dairy cows.
30591340	3	116	from	meal	808:811	arg1	diet					820:823	the diet	816:823	the diet	816:823	The main plots were created by randomly assigning 9 cows to diets of 11 or 9% RDP obtained by altering the percentage of soybean meal, expeller soybean meal, and blood meal in the diet.
30591340	10	117	theme	lower	1919:1923	arg1	concentration					1941:1953	lower ruminal ammonia concentration	1919:1953	lower ruminal ammonia concentration (7.2 vs. 12.3 mg/dL)	1919:1974	Treatments did not influence milk production responses, but cows fed 9% RDP diets had lower ruminal ammonia concentration (7.2 vs. 12.3 mg/dL) and tended to excrete less urinary purine derivatives (428 vs. 493 mmol/d) compared with cows fed 11% RDP diets, suggesting lower ruminal synthesis of microbial protein.
30591340	10	117	theme	lower	1919:1923	arg1	mg/dL					1969:1973	7.2 vs. 12.3 mg/dL	1956:1973	7.2 vs. 12.3 mg/dL	1956:1973	Treatments did not influence milk production responses, but cows fed 9% RDP diets had lower ruminal ammonia concentration (7.2 vs. 12.3 mg/dL) and tended to excrete less urinary purine derivatives (428 vs. 493 mmol/d) compared with cows fed 11% RDP diets, suggesting lower ruminal synthesis of microbial protein.
30591340	5	118	theme	refined	918:924	arg1	ratio					942:946	10:0 refined starch:dextrose ratio	913:946	10:0 refined starch:dextrose ratio (% of dietary dry matter)	913:972	In the subplots, the effects of 0:10, 5:5, and 10:0 refined starch:dextrose ratio (% of dietary dry matter) were determined in three 3 × 3 Latin squares by randomly assigning the 9 cows in each RDP level into squares.
30591340	8	119	theme	acids	1526:1530	arg1	efficiency					1598:1607	N use efficiency	1592:1607	N use efficiency (milk N/intake N)	1592:1625	Replacing refined starch with dextrose increased DMI, the molar percentage of ruminal butyrate and valerate, daily CH4 production (g/d), and fecal N and decreased the molar percentage of ruminal branched-chain volatile fatty acids, feed efficiency (fat- and protein-corrected milk/DMI), and N use efficiency (milk N/intake N) but did not influence nutrient digestibility.
30591340	8	119	theme	acids	1526:1530	arg1	N					1624:1624	milk N/intake N	1610:1624	milk N/intake N	1610:1624	Replacing refined starch with dextrose increased DMI, the molar percentage of ruminal butyrate and valerate, daily CH4 production (g/d), and fecal N and decreased the molar percentage of ruminal branched-chain volatile fatty acids, feed efficiency (fat- and protein-corrected milk/DMI), and N use efficiency (milk N/intake N) but did not influence nutrient digestibility.
30591340	8	119	theme	acids	1526:1530	arg1	percentage					1474:1483	the molar percentage	1464:1483	the molar percentage of ruminal branched-chain volatile fatty acids	1464:1530	Replacing refined starch with dextrose increased DMI, the molar percentage of ruminal butyrate and valerate, daily CH4 production (g/d), and fecal N and decreased the molar percentage of ruminal branched-chain volatile fatty acids, feed efficiency (fat- and protein-corrected milk/DMI), and N use efficiency (milk N/intake N) but did not influence nutrient digestibility.
30591340	8	119	theme	acids	1526:1530	arg1	efficiency					1538:1547	feed efficiency	1533:1547	feed efficiency (fat- and protein-corrected milk/DMI)	1533:1585	Replacing refined starch with dextrose increased DMI, the molar percentage of ruminal butyrate and valerate, daily CH4 production (g/d), and fecal N and decreased the molar percentage of ruminal branched-chain volatile fatty acids, feed efficiency (fat- and protein-corrected milk/DMI), and N use efficiency (milk N/intake N) but did not influence nutrient digestibility.
30591340	3	120	theme	meal	769:772	arg1	percentage					747:756	the percentage	743:756	the percentage of soybean meal, expeller soybean meal,	743:796	The main plots were created by randomly assigning 9 cows to diets of 11 or 9% RDP obtained by altering the percentage of soybean meal, expeller soybean meal, and blood meal in the diet.
30591340	3	120	theme	meal	769:772	arg1	meal					808:811	blood meal	802:811	blood meal in the diet	802:823	The main plots were created by randomly assigning 9 cows to diets of 11 or 9% RDP obtained by altering the percentage of soybean meal, expeller soybean meal, and blood meal in the diet.
30591340	5	121	theme	RDP	1060:1062	arg1	level					1064:1068	each RDP level	1055:1068	each RDP level	1055:1068	In the subplots, the effects of 0:10, 5:5, and 10:0 refined starch:dextrose ratio (% of dietary dry matter) were determined in three 3 × 3 Latin squares by randomly assigning the 9 cows in each RDP level into squares.
30591340	3	122	theme	RDP	718:720	arg1	diets					700:704	diets	700:704	diets of 11 or 9% RDP obtained by altering the percentage of soybean meal, expeller soybean meal, and blood meal in the diet	700:823	The main plots were created by randomly assigning 9 cows to diets of 11 or 9% RDP obtained by altering the percentage of soybean meal, expeller soybean meal, and blood meal in the diet.
30591340	5	123	theme	matter	966:971	arg1	matter					966:971	dietary dry matter	954:971	dietary dry matter	954:971	In the subplots, the effects of 0:10, 5:5, and 10:0 refined starch:dextrose ratio (% of dietary dry matter) were determined in three 3 × 3 Latin squares by randomly assigning the 9 cows in each RDP level into squares.
30591340	5	123	theme	matter	966:971	arg1	%					949:949	%	949:949	% of dietary dry matter	949:971	In the subplots, the effects of 0:10, 5:5, and 10:0 refined starch:dextrose ratio (% of dietary dry matter) were determined in three 3 × 3 Latin squares by randomly assigning the 9 cows in each RDP level into squares.
30591340	8	124	theme	feed	1533:1536	arg1	efficiency					1538:1547	feed efficiency	1533:1547	feed efficiency (fat- and protein-corrected milk/DMI)	1533:1585	Replacing refined starch with dextrose increased DMI, the molar percentage of ruminal butyrate and valerate, daily CH4 production (g/d), and fecal N and decreased the molar percentage of ruminal branched-chain volatile fatty acids, feed efficiency (fat- and protein-corrected milk/DMI), and N use efficiency (milk N/intake N) but did not influence nutrient digestibility.
30591340	3	125	dep	%	716:716	arg1	11					709:710	11	709:710	11	709:710	The main plots were created by randomly assigning 9 cows to diets of 11 or 9% RDP obtained by altering the percentage of soybean meal, expeller soybean meal, and blood meal in the diet.
30591340	3	125	dep	%	716:716	arg1	9					715:715	9	715:715	9	715:715	The main plots were created by randomly assigning 9 cows to diets of 11 or 9% RDP obtained by altering the percentage of soybean meal, expeller soybean meal, and blood meal in the diet.
30591340	10	126	theme	ammonia	1933:1939	arg1	concentration					1941:1953	lower ruminal ammonia concentration	1919:1953	lower ruminal ammonia concentration (7.2 vs. 12.3 mg/dL)	1919:1974	Treatments did not influence milk production responses, but cows fed 9% RDP diets had lower ruminal ammonia concentration (7.2 vs. 12.3 mg/dL) and tended to excrete less urinary purine derivatives (428 vs. 493 mmol/d) compared with cows fed 11% RDP diets, suggesting lower ruminal synthesis of microbial protein.
30591340	10	126	theme	ammonia	1933:1939	arg1	mg/dL					1969:1973	7.2 vs. 12.3 mg/dL	1956:1973	7.2 vs. 12.3 mg/dL	1956:1973	Treatments did not influence milk production responses, but cows fed 9% RDP diets had lower ruminal ammonia concentration (7.2 vs. 12.3 mg/dL) and tended to excrete less urinary purine derivatives (428 vs. 493 mmol/d) compared with cows fed 11% RDP diets, suggesting lower ruminal synthesis of microbial protein.
30591340	12	127	theme	protein-corrected	2440:2456	arg1	milk					2458:2461	protein-corrected milk	2440:2461	protein-corrected milk	2440:2461	In this study, treatments did not affect yield (20.0 g of CH4/kg of DMI) or intensity (13.1 g of CH4/kg of fat- and protein-corrected milk), but methane production (g of CH4/d) was 7.0% lower and N use efficiency (conversion of intake N into milk protein) was 7.8% higher for cows fed a diet of 28.1% starch and 4.6% water-soluble carbohydrate compared with diets with lower starch and higher water-soluble carbohydrate contents.
30591340	11	128	theme	iso-nitrogenous	2175:2189	arg1	diets					2191:2195	iso-nitrogenous diets	2175:2195	iso-nitrogenous diets	2175:2195	Reducing the level of RDP in iso-nitrogenous diets had no effect on nutrient apparent total-tract digestibility, manure excretion and composition, N balance, and CH4 production.
30591340	9	129	theme	ruminal	1746:1752	arg1	propionate					1754:1763	ruminal propionate	1746:1763	ruminal propionate	1746:1763	Enteric CH4 production was negatively related to the molar percentage of ruminal propionate but positively related to the molar percentage of ruminal butyrate.
30591340	12	130	dep	higher	2589:2594	arg1	%					2587:2587	7.8% higher	2584:2594	7.8% higher for cows fed a diet of 28.1% starch	2584:2630	In this study, treatments did not affect yield (20.0 g of CH4/kg of DMI) or intensity (13.1 g of CH4/kg of fat- and protein-corrected milk), but methane production (g of CH4/d) was 7.0% lower and N use efficiency (conversion of intake N into milk protein) was 7.8% higher for cows fed a diet of 28.1% starch and 4.6% water-soluble carbohydrate compared with diets with lower starch and higher water-soluble carbohydrate contents.
30591340	0	131	theme	lactation	97:105	arg1	performance					107:117	lactation performance	97:117	lactation performance	97:117	Starch and dextrose at 2 levels of rumen-degradable protein in iso-nitrogenous diets: Effects on lactation performance, ruminal measurements, methane emission, digestibility, and nitrogen balance of dairy cows.
30591340	1	132	theme	level	338:342	arg1	effects					248:254	the effects	244:254	the effects of readily rumen-available carbohydrate source (refined starch vs. dextrose), the level of rumen-degradable protein (RDP), and their interaction on lactation performance, ruminal measurements, enteric methane (CH4) emission, nutrient digestibility, and nitrogen (N) balance in lactating dairy cows	244:552	Our objectives were to determine the effects of readily rumen-available carbohydrate source (refined starch vs. dextrose), the level of rumen-degradable protein (RDP), and their interaction on lactation performance, ruminal measurements, enteric methane (CH4) emission, nutrient digestibility, and nitrogen (N) balance in lactating dairy cows.
30591340	4	133	theme	%	849:849	arg1	protein					857:863	16.4% crude protein	845:863	16.4% crude protein	845:863	All diets included 16.4% crude protein.
30591340	0	134	from	levels	25:30	arg1	dextrose					11:18	dextrose	11:18	dextrose	11:18	Starch and dextrose at 2 levels of rumen-degradable protein in iso-nitrogenous diets: Effects on lactation performance, ruminal measurements, methane emission, digestibility, and nitrogen balance of dairy cows.
30591340	0	134	from	levels	25:30	arg1	Starch					0:5	Starch	0:5	Starch	0:5	Starch and dextrose at 2 levels of rumen-degradable protein in iso-nitrogenous diets: Effects on lactation performance, ruminal measurements, methane emission, digestibility, and nitrogen balance of dairy cows.
30591340	8	135	theme	molar	1359:1363	arg1	percentage					1365:1374	the molar percentage	1355:1374	the molar percentage of ruminal butyrate and valerate	1355:1407	Replacing refined starch with dextrose increased DMI, the molar percentage of ruminal butyrate and valerate, daily CH4 production (g/d), and fecal N and decreased the molar percentage of ruminal branched-chain volatile fatty acids, feed efficiency (fat- and protein-corrected milk/DMI), and N use efficiency (milk N/intake N) but did not influence nutrient digestibility.
30591340	1	136	theme	nitrogen	509:516	arg1	balance					522:528	nitrogen (N) balance	509:528	nitrogen (N) balance	509:528	Our objectives were to determine the effects of readily rumen-available carbohydrate source (refined starch vs. dextrose), the level of rumen-degradable protein (RDP), and their interaction on lactation performance, ruminal measurements, enteric methane (CH4) emission, nutrient digestibility, and nitrogen (N) balance in lactating dairy cows.
30591340	10	137	theme	RDP	2078:2080	arg1	diets					2082:2086	11% RDP diets	2074:2086	11% RDP diets	2074:2086	Treatments did not influence milk production responses, but cows fed 9% RDP diets had lower ruminal ammonia concentration (7.2 vs. 12.3 mg/dL) and tended to excrete less urinary purine derivatives (428 vs. 493 mmol/d) compared with cows fed 11% RDP diets, suggesting lower ruminal synthesis of microbial protein.
30591340	5	138	theme	dietary	954:960	arg1	matter					966:971	dietary dry matter	954:971	dietary dry matter	954:971	In the subplots, the effects of 0:10, 5:5, and 10:0 refined starch:dextrose ratio (% of dietary dry matter) were determined in three 3 × 3 Latin squares by randomly assigning the 9 cows in each RDP level into squares.
30591340	1	139	from	emission	471:478	arg1	cows					549:552	lactating dairy cows	533:552	lactating dairy cows	533:552	Our objectives were to determine the effects of readily rumen-available carbohydrate source (refined starch vs. dextrose), the level of rumen-degradable protein (RDP), and their interaction on lactation performance, ruminal measurements, enteric methane (CH4) emission, nutrient digestibility, and nitrogen (N) balance in lactating dairy cows.
30591340	10	140	theme	ruminal	2106:2112	arg1	synthesis					2114:2122	lower ruminal synthesis	2100:2122	lower ruminal synthesis of microbial protein	2100:2143	Treatments did not influence milk production responses, but cows fed 9% RDP diets had lower ruminal ammonia concentration (7.2 vs. 12.3 mg/dL) and tended to excrete less urinary purine derivatives (428 vs. 493 mmol/d) compared with cows fed 11% RDP diets, suggesting lower ruminal synthesis of microbial protein.
30591340	11	141	theme	CH4	2308:2310	arg1	production					2312:2321	CH4 production	2308:2321	CH4 production	2308:2321	Reducing the level of RDP in iso-nitrogenous diets had no effect on nutrient apparent total-tract digestibility, manure excretion and composition, N balance, and CH4 production.
30591340	2	142	theme	split-plot	622:631	arg1	study					633:637	this split-plot study	617:637	this split-plot study	617:637	Eighteen mid-lactation multiparous Holstein cows were used in this split-plot study.
30591340	1	143	theme	N	519:519	arg1	balance					522:528	nitrogen (N) balance	509:528	nitrogen (N) balance	509:528	Our objectives were to determine the effects of readily rumen-available carbohydrate source (refined starch vs. dextrose), the level of rumen-degradable protein (RDP), and their interaction on lactation performance, ruminal measurements, enteric methane (CH4) emission, nutrient digestibility, and nitrogen (N) balance in lactating dairy cows.
30591340	12	144	with	diets	2682:2686	arg1	contents					2744:2751	lower starch and higher water-soluble carbohydrate contents	2693:2751	lower starch and higher water-soluble carbohydrate contents	2693:2751	In this study, treatments did not affect yield (20.0 g of CH4/kg of DMI) or intensity (13.1 g of CH4/kg of fat- and protein-corrected milk), but methane production (g of CH4/d) was 7.0% lower and N use efficiency (conversion of intake N into milk protein) was 7.8% higher for cows fed a diet of 28.1% starch and 4.6% water-soluble carbohydrate compared with diets with lower starch and higher water-soluble carbohydrate contents.
30591340	10	145	theme	ruminal	1925:1931	arg1	concentration					1941:1953	lower ruminal ammonia concentration	1919:1953	lower ruminal ammonia concentration (7.2 vs. 12.3 mg/dL)	1919:1974	Treatments did not influence milk production responses, but cows fed 9% RDP diets had lower ruminal ammonia concentration (7.2 vs. 12.3 mg/dL) and tended to excrete less urinary purine derivatives (428 vs. 493 mmol/d) compared with cows fed 11% RDP diets, suggesting lower ruminal synthesis of microbial protein.
30591340	10	145	theme	ruminal	1925:1931	arg1	mg/dL					1969:1973	7.2 vs. 12.3 mg/dL	1956:1973	7.2 vs. 12.3 mg/dL	1956:1973	Treatments did not influence milk production responses, but cows fed 9% RDP diets had lower ruminal ammonia concentration (7.2 vs. 12.3 mg/dL) and tended to excrete less urinary purine derivatives (428 vs. 493 mmol/d) compared with cows fed 11% RDP diets, suggesting lower ruminal synthesis of microbial protein.
30591340	1	146	from	balance	522:528	arg1	cows					549:552	lactating dairy cows	533:552	lactating dairy cows	533:552	Our objectives were to determine the effects of readily rumen-available carbohydrate source (refined starch vs. dextrose), the level of rumen-degradable protein (RDP), and their interaction on lactation performance, ruminal measurements, enteric methane (CH4) emission, nutrient digestibility, and nitrogen (N) balance in lactating dairy cows.
30591340	12	147	theme	fat-	2431:2434	arg1	CH4/kg					2421:2426	CH4/kg	2421:2426	CH4/kg of fat- and protein-corrected milk	2421:2461	In this study, treatments did not affect yield (20.0 g of CH4/kg of DMI) or intensity (13.1 g of CH4/kg of fat- and protein-corrected milk), but methane production (g of CH4/d) was 7.0% lower and N use efficiency (conversion of intake N into milk protein) was 7.8% higher for cows fed a diet of 28.1% starch and 4.6% water-soluble carbohydrate compared with diets with lower starch and higher water-soluble carbohydrate contents.
30591340	10	148	theme	production	1867:1876	arg1	responses					1878:1886	milk production responses	1862:1886	milk production responses	1862:1886	Treatments did not influence milk production responses, but cows fed 9% RDP diets had lower ruminal ammonia concentration (7.2 vs. 12.3 mg/dL) and tended to excrete less urinary purine derivatives (428 vs. 493 mmol/d) compared with cows fed 11% RDP diets, suggesting lower ruminal synthesis of microbial protein.
30591340	12	149	dep	intensity	2400:2408	arg1	g					2416:2416	13.1 g	2411:2416	13.1 g of CH4/kg of fat- and protein-corrected milk	2411:2461	In this study, treatments did not affect yield (20.0 g of CH4/kg of DMI) or intensity (13.1 g of CH4/kg of fat- and protein-corrected milk), but methane production (g of CH4/d) was 7.0% lower and N use efficiency (conversion of intake N into milk protein) was 7.8% higher for cows fed a diet of 28.1% starch and 4.6% water-soluble carbohydrate compared with diets with lower starch and higher water-soluble carbohydrate contents.
30591340	1	150	theme	carbohydrate	283:294	arg1	source					296:301	readily rumen-available carbohydrate source	259:301	readily rumen-available carbohydrate source (refined starch vs. dextrose)	259:331	Our objectives were to determine the effects of readily rumen-available carbohydrate source (refined starch vs. dextrose), the level of rumen-degradable protein (RDP), and their interaction on lactation performance, ruminal measurements, enteric methane (CH4) emission, nutrient digestibility, and nitrogen (N) balance in lactating dairy cows.
30591340	10	151	theme	protein	2137:2143	arg1	synthesis					2114:2122	lower ruminal synthesis	2100:2122	lower ruminal synthesis of microbial protein	2100:2143	Treatments did not influence milk production responses, but cows fed 9% RDP diets had lower ruminal ammonia concentration (7.2 vs. 12.3 mg/dL) and tended to excrete less urinary purine derivatives (428 vs. 493 mmol/d) compared with cows fed 11% RDP diets, suggesting lower ruminal synthesis of microbial protein.
30591340	12	152	theme	water-soluble	2717:2729	arg1	contents					2744:2751	lower starch and higher water-soluble carbohydrate contents	2693:2751	lower starch and higher water-soluble carbohydrate contents	2693:2751	In this study, treatments did not affect yield (20.0 g of CH4/kg of DMI) or intensity (13.1 g of CH4/kg of fat- and protein-corrected milk), but methane production (g of CH4/d) was 7.0% lower and N use efficiency (conversion of intake N into milk protein) was 7.8% higher for cows fed a diet of 28.1% starch and 4.6% water-soluble carbohydrate compared with diets with lower starch and higher water-soluble carbohydrate contents.
30591340	1	153	theme	methane	457:463	arg1	emission					471:478	enteric methane (CH4) emission	449:478	enteric methane (CH4) emission	449:478	Our objectives were to determine the effects of readily rumen-available carbohydrate source (refined starch vs. dextrose), the level of rumen-degradable protein (RDP), and their interaction on lactation performance, ruminal measurements, enteric methane (CH4) emission, nutrient digestibility, and nitrogen (N) balance in lactating dairy cows.
30591340	10	154	theme	%	2076:2076	arg1	diets					2082:2086	11% RDP diets	2074:2086	11% RDP diets	2074:2086	Treatments did not influence milk production responses, but cows fed 9% RDP diets had lower ruminal ammonia concentration (7.2 vs. 12.3 mg/dL) and tended to excrete less urinary purine derivatives (428 vs. 493 mmol/d) compared with cows fed 11% RDP diets, suggesting lower ruminal synthesis of microbial protein.
30591340	0	155	theme	rumen-degradable	35:50	arg1	protein					52:58	rumen-degradable protein	35:58	rumen-degradable protein	35:58	Starch and dextrose at 2 levels of rumen-degradable protein in iso-nitrogenous diets: Effects on lactation performance, ruminal measurements, methane emission, digestibility, and nitrogen balance of dairy cows.
30591340	1	156	dep	source	296:301	arg1	dextrose					323:330	dextrose	323:330	dextrose	323:330	Our objectives were to determine the effects of readily rumen-available carbohydrate source (refined starch vs. dextrose), the level of rumen-degradable protein (RDP), and their interaction on lactation performance, ruminal measurements, enteric methane (CH4) emission, nutrient digestibility, and nitrogen (N) balance in lactating dairy cows.
30591340	1	156	dep	source	296:301	arg1	starch					312:317	refined starch	304:317	refined starch	304:317	Our objectives were to determine the effects of readily rumen-available carbohydrate source (refined starch vs. dextrose), the level of rumen-degradable protein (RDP), and their interaction on lactation performance, ruminal measurements, enteric methane (CH4) emission, nutrient digestibility, and nitrogen (N) balance in lactating dairy cows.
30591340	9	157	theme	ruminal	1815:1821	arg1	butyrate					1823:1830	ruminal butyrate	1815:1830	ruminal butyrate	1815:1830	Enteric CH4 production was negatively related to the molar percentage of ruminal propionate but positively related to the molar percentage of ruminal butyrate.
30591340	1	158	theme	CH4	466:468	arg1	emission					471:478	enteric methane (CH4) emission	449:478	enteric methane (CH4) emission	449:478	Our objectives were to determine the effects of readily rumen-available carbohydrate source (refined starch vs. dextrose), the level of rumen-degradable protein (RDP), and their interaction on lactation performance, ruminal measurements, enteric methane (CH4) emission, nutrient digestibility, and nitrogen (N) balance in lactating dairy cows.
30591340	8	159	theme	CH4	1416:1418	arg1	production					1420:1429	daily CH4 production	1410:1429	daily CH4 production (g/d)	1410:1435	Replacing refined starch with dextrose increased DMI, the molar percentage of ruminal butyrate and valerate, daily CH4 production (g/d), and fecal N and decreased the molar percentage of ruminal branched-chain volatile fatty acids, feed efficiency (fat- and protein-corrected milk/DMI), and N use efficiency (milk N/intake N) but did not influence nutrient digestibility.
30591340	8	159	theme	CH4	1416:1418	arg1	g/d					1432:1434	g/d	1432:1434	g/d	1432:1434	Replacing refined starch with dextrose increased DMI, the molar percentage of ruminal butyrate and valerate, daily CH4 production (g/d), and fecal N and decreased the molar percentage of ruminal branched-chain volatile fatty acids, feed efficiency (fat- and protein-corrected milk/DMI), and N use efficiency (milk N/intake N) but did not influence nutrient digestibility.
30591340	10	160	theme	%	1903:1903	arg1	diets					1909:1913	9% RDP diets	1902:1913	9% RDP diets	1902:1913	Treatments did not influence milk production responses, but cows fed 9% RDP diets had lower ruminal ammonia concentration (7.2 vs. 12.3 mg/dL) and tended to excrete less urinary purine derivatives (428 vs. 493 mmol/d) compared with cows fed 11% RDP diets, suggesting lower ruminal synthesis of microbial protein.
30591340	7	161	theme	N	1278:1278	arg1	DMI					1243:1245	DMI	1243:1245	DMI	1243:1245	Carbohydrate source × RDP level interaction tended to influence dry matter intake (DMI), the concentration of urinary N, and urinary urea-N.
30591340	7	161	theme	N	1278:1278	arg1	urea-N					1293:1298	urinary urea-N	1285:1298	urinary urea-N	1285:1298	Carbohydrate source × RDP level interaction tended to influence dry matter intake (DMI), the concentration of urinary N, and urinary urea-N.
30591340	7	161	theme	N	1278:1278	arg1	intake					1235:1240	dry matter intake	1224:1240	dry matter intake (DMI)	1224:1246	Carbohydrate source × RDP level interaction tended to influence dry matter intake (DMI), the concentration of urinary N, and urinary urea-N.
30591340	7	161	theme	N	1278:1278	arg1	concentration					1253:1265	the concentration	1249:1265	the concentration of urinary N	1249:1278	Carbohydrate source × RDP level interaction tended to influence dry matter intake (DMI), the concentration of urinary N, and urinary urea-N.
30591340	1	162	from	performance	414:424	arg1	cows					549:552	lactating dairy cows	533:552	lactating dairy cows	533:552	Our objectives were to determine the effects of readily rumen-available carbohydrate source (refined starch vs. dextrose), the level of rumen-degradable protein (RDP), and their interaction on lactation performance, ruminal measurements, enteric methane (CH4) emission, nutrient digestibility, and nitrogen (N) balance in lactating dairy cows.
30591340	8	163	theme	ruminal	1379:1385	arg1	butyrate					1387:1394	ruminal butyrate	1379:1394	ruminal butyrate	1379:1394	Replacing refined starch with dextrose increased DMI, the molar percentage of ruminal butyrate and valerate, daily CH4 production (g/d), and fecal N and decreased the molar percentage of ruminal branched-chain volatile fatty acids, feed efficiency (fat- and protein-corrected milk/DMI), and N use efficiency (milk N/intake N) but did not influence nutrient digestibility.
30591340	12	164	theme	water-soluble	2641:2653	arg1	carbohydrate					2655:2666	water-soluble carbohydrate	2641:2666	4.6% water-soluble carbohydrate	2636:2666	In this study, treatments did not affect yield (20.0 g of CH4/kg of DMI) or intensity (13.1 g of CH4/kg of fat- and protein-corrected milk), but methane production (g of CH4/d) was 7.0% lower and N use efficiency (conversion of intake N into milk protein) was 7.8% higher for cows fed a diet of 28.1% starch and 4.6% water-soluble carbohydrate compared with diets with lower starch and higher water-soluble carbohydrate contents.
30591340	1	165	theme	nutrient	481:488	arg1	digestibility					490:502	nutrient digestibility	481:502	nutrient digestibility	481:502	Our objectives were to determine the effects of readily rumen-available carbohydrate source (refined starch vs. dextrose), the level of rumen-degradable protein (RDP), and their interaction on lactation performance, ruminal measurements, enteric methane (CH4) emission, nutrient digestibility, and nitrogen (N) balance in lactating dairy cows.
31125305	2	0	dep	Gram-stain-positive	52:70	arg1	endospore-forming					111:127	endospore-forming	111:127	endospore-forming	111:127	A Gram-stain-positive, strictly aerobic, rod-shaped, motile, endospore-forming strain, SYSU K30001T, was isolated from a soil sample collected from a cave in Xingyi county, Guizhou province, south-west China.
31125305	2	0	dep	Gram-stain-positive	52:70	arg1	motile					103:108	motile	103:108	motile	103:108	A Gram-stain-positive, strictly aerobic, rod-shaped, motile, endospore-forming strain, SYSU K30001T, was isolated from a soil sample collected from a cave in Xingyi county, Guizhou province, south-west China.
31125305	2	0	dep	Gram-stain-positive	52:70	arg1	rod-shaped					91:100	rod-shaped	91:100	rod-shaped	91:100	A Gram-stain-positive, strictly aerobic, rod-shaped, motile, endospore-forming strain, SYSU K30001T, was isolated from a soil sample collected from a cave in Xingyi county, Guizhou province, south-west China.
31125305	2	0	dep	Gram-stain-positive	52:70	arg1	aerobic					82:88	aerobic	82:88	aerobic	82:88	A Gram-stain-positive, strictly aerobic, rod-shaped, motile, endospore-forming strain, SYSU K30001T, was isolated from a soil sample collected from a cave in Xingyi county, Guizhou province, south-west China.
31125305	5	1	from	MK-7	718:721	arg1	peptidoglycan					700:712	the cell-wall peptidoglycan	686:712	the cell-wall peptidoglycan	686:712	Strain SYSU K30001T contained meso-2,6-diaminopimelic acid in the cell-wall peptidoglycan and MK-7 as the only isoprenoid quinone present.
31125305	4	2	theme	0-3 	573:576	arg1	NaCl					585:588	0-3 % (w/v) NaCl	573:588	0-3 % (w/v) NaCl (optimum in the absence of NaCl)	573:621	Growth occurred at pH 6.0-9.0 (optimum, pH 7.0), at 28-55 °C (optimum, 28 °C) and in the presence of 0-3 % (w/v) NaCl (optimum in the absence of NaCl).
31125305	9	3	dep	K30001T	1076:1082	arg1	19096T					1108:1113	DSM 19096T	1104:1113	DSM 19096T	1104:1113	The average nucleotide identity values between SYSU K30001T and B. panaciterrae DSM 19096T were 72.1 % (ANIb) and 83.1 % (ANIm), which were below the cut-off level (95-96 %) for species delineation.
31125305	6	4	theme	polar	767:771	arg1	lipids					773:778	The polar lipids	763:778	The polar lipids	763:778	The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified glycolipid.
31125305	10	5	theme	phenotypic	1232:1241	arg1	characterizations					1273:1289	phenotypic, chemotaxonomic and molecular characterizations	1232:1289	phenotypic, chemotaxonomic and molecular characterizations	1232:1289	Based on phenotypic, chemotaxonomic and molecular characterizations, strain SYSU K30001T represents a novel species of the genus Bacillus, for which the name Bacillus antri sp.
31125305	3	6	theme	panaciterrae	449:460	arg1	strain					430:435	the type strain	421:435	the type strain of Bacillus panaciterrae (98.1 %)	421:469	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SYSU K30001T belonged to the genus Bacillus, with the highest sequence similarity to the type strain of Bacillus panaciterrae (98.1 %).
31125305	9	7	theme	species	1202:1208	arg1	delineation					1210:1220	species delineation	1202:1220	species delineation	1202:1220	The average nucleotide identity values between SYSU K30001T and B. panaciterrae DSM 19096T were 72.1 % (ANIb) and 83.1 % (ANIm), which were below the cut-off level (95-96 %) for species delineation.
31125305	8	8	theme	39.8 mol	1013:1020	arg1	G+C content					997:1007	The genome G+C content	986:1007	The genome G+C content	986:1007	The genome G+C content was 39.8 mol%.
31125305	8	8	theme	39.8 mol	1013:1020	arg1	%					1021:1021	39.8 mol%	1013:1021	39.8 mol%	1013:1021	The genome G+C content was 39.8 mol%.
31125305	5	9	contain	contained	644:652	arg1	Strain					624:629	Strain SYSU K30001T	624:642	Strain SYSU K30001T	624:642	Strain SYSU K30001T contained meso-2,6-diaminopimelic acid in the cell-wall peptidoglycan and MK-7 as the only isoprenoid quinone present.
31125305	5	9	contain	contained	644:652	arg2	acid					678:681	meso-2,6-diaminopimelic acid	654:681	meso-2,6-diaminopimelic acid in the cell-wall peptidoglycan	654:712	Strain SYSU K30001T contained meso-2,6-diaminopimelic acid in the cell-wall peptidoglycan and MK-7 as the only isoprenoid quinone present.
31125305	5	9	contain	contained	644:652	arg2	MK-7					718:721	MK-7	718:721	MK-7	718:721	Strain SYSU K30001T contained meso-2,6-diaminopimelic acid in the cell-wall peptidoglycan and MK-7 as the only isoprenoid quinone present.
31125305	5	9	contain	contained	644:652	arg2	present					754:760	the only isoprenoid quinone present	726:760	the only isoprenoid quinone present	726:760	Strain SYSU K30001T contained meso-2,6-diaminopimelic acid in the cell-wall peptidoglycan and MK-7 as the only isoprenoid quinone present.
31125305	9	10	theme	nucleotide	1036:1045	arg1	values					1056:1061	The average nucleotide identity values	1024:1061	The average nucleotide identity values between SYSU K30001T and B. panaciterrae DSM 19096T	1024:1113	The average nucleotide identity values between SYSU K30001T and B. panaciterrae DSM 19096T were 72.1 % (ANIb) and 83.1 % (ANIm), which were below the cut-off level (95-96 %) for species delineation.
31125305	9	10	theme	nucleotide	1036:1045	arg1	%					1125:1125	72.1 %	1120:1125	72.1 % (ANIb)	1120:1132	The average nucleotide identity values between SYSU K30001T and B. panaciterrae DSM 19096T were 72.1 % (ANIb) and 83.1 % (ANIm), which were below the cut-off level (95-96 %) for species delineation.
31125305	1	11	theme	cave	39:42	arg1	soil					44:47	cave soil	39:47	cave soil	39:47	nov., isolated from cave soil.
31125305	6	12	theme	unidentified	871:882	arg1	glycolipid					884:893	an unidentified glycolipid	868:893	an unidentified glycolipid	868:893	The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified glycolipid.
31125305	7	13	dep	iso-C15 	923:930	arg1	iso-C17 					973:980	iso-C17 	973:980	iso-C17 	973:980	The major fatty acids were iso-C15 : 0, iso-C17 : 1ω10c, anteiso-C14 : 0 and iso-C17 : 0.
31125305	7	13	dep	iso-C15 	923:930	arg1	 0					966:967	 0	966:967	 0	966:967	The major fatty acids were iso-C15 : 0, iso-C17 : 1ω10c, anteiso-C14 : 0 and iso-C17 : 0.
31125305	7	13	dep	iso-C15 	923:930	arg1	 0					932:933	 0	932:933	 0	932:933	The major fatty acids were iso-C15 : 0, iso-C17 : 1ω10c, anteiso-C14 : 0 and iso-C17 : 0.
31125305	7	13	dep	iso-C15 	923:930	arg1	 0					982:983	 0	982:983	 0	982:983	The major fatty acids were iso-C15 : 0, iso-C17 : 1ω10c, anteiso-C14 : 0 and iso-C17 : 0.
31125305	4	14	from	absence	606:612	arg1	optimum					591:597	optimum	591:597	optimum	591:597	Growth occurred at pH 6.0-9.0 (optimum, pH 7.0), at 28-55 °C (optimum, 28 °C) and in the presence of 0-3 % (w/v) NaCl (optimum in the absence of NaCl).
31125305	5	15	theme	quinone	746:752	arg1	MK-7					718:721	MK-7	718:721	MK-7	718:721	Strain SYSU K30001T contained meso-2,6-diaminopimelic acid in the cell-wall peptidoglycan and MK-7 as the only isoprenoid quinone present.
31125305	5	15	theme	quinone	746:752	arg1	present					754:760	the only isoprenoid quinone present	726:760	the only isoprenoid quinone present	726:760	Strain SYSU K30001T contained meso-2,6-diaminopimelic acid in the cell-wall peptidoglycan and MK-7 as the only isoprenoid quinone present.
31125305	5	15	theme	quinone	746:752	arg1	acid					678:681	meso-2,6-diaminopimelic acid	654:681	meso-2,6-diaminopimelic acid in the cell-wall peptidoglycan	654:712	Strain SYSU K30001T contained meso-2,6-diaminopimelic acid in the cell-wall peptidoglycan and MK-7 as the only isoprenoid quinone present.
31125305	12	16	theme	SYSU	1437:1440	arg1	1.13871T					1470:1477	=KCTC 33954T=CGMCC 1.13871T	1451:1477	=KCTC 33954T=CGMCC 1.13871T	1451:1477	The type strain is SYSU K30001T (=KCTC 33954T=CGMCC 1.13871T).
31125305	12	16	theme	SYSU	1437:1440	arg1	strain					1427:1432	The type strain	1418:1432	The type strain	1418:1432	The type strain is SYSU K30001T (=KCTC 33954T=CGMCC 1.13871T).
31125305	12	16	theme	SYSU	1437:1440	arg1	K30001T					1442:1448	SYSU K30001T	1437:1448	SYSU K30001T (=KCTC 33954T=CGMCC 1.13871T)	1437:1478	The type strain is SYSU K30001T (=KCTC 33954T=CGMCC 1.13871T).
31125305	4	17	theme	w/v	580:582	arg1	NaCl					585:588	0-3 % (w/v) NaCl	573:588	0-3 % (w/v) NaCl (optimum in the absence of NaCl)	573:621	Growth occurred at pH 6.0-9.0 (optimum, pH 7.0), at 28-55 °C (optimum, 28 °C) and in the presence of 0-3 % (w/v) NaCl (optimum in the absence of NaCl).
31125305	3	18	theme	rRNA	294:297	arg1	sequences					304:312	16S rRNA gene sequences	290:312	16S rRNA gene sequences	290:312	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SYSU K30001T belonged to the genus Bacillus, with the highest sequence similarity to the type strain of Bacillus panaciterrae (98.1 %).
31125305	5	19	from	acid	678:681	arg1	peptidoglycan					700:712	the cell-wall peptidoglycan	686:712	the cell-wall peptidoglycan	686:712	Strain SYSU K30001T contained meso-2,6-diaminopimelic acid in the cell-wall peptidoglycan and MK-7 as the only isoprenoid quinone present.
31125305	5	20	dep	Strain	624:629	arg1	K30001T					636:642	K30001T	636:642	K30001T	636:642	Strain SYSU K30001T contained meso-2,6-diaminopimelic acid in the cell-wall peptidoglycan and MK-7 as the only isoprenoid quinone present.
31125305	9	21	theme	identity	1047:1054	arg1	values					1056:1061	The average nucleotide identity values	1024:1061	The average nucleotide identity values between SYSU K30001T and B. panaciterrae DSM 19096T	1024:1113	The average nucleotide identity values between SYSU K30001T and B. panaciterrae DSM 19096T were 72.1 % (ANIb) and 83.1 % (ANIm), which were below the cut-off level (95-96 %) for species delineation.
31125305	9	21	theme	identity	1047:1054	arg1	%					1125:1125	72.1 %	1120:1125	72.1 % (ANIb)	1120:1132	The average nucleotide identity values between SYSU K30001T and B. panaciterrae DSM 19096T were 72.1 % (ANIb) and 83.1 % (ANIm), which were below the cut-off level (95-96 %) for species delineation.
31125305	4	22	theme	NaCl	617:620	arg1	absence					606:612	the absence	602:612	the absence of NaCl	602:620	Growth occurred at pH 6.0-9.0 (optimum, pH 7.0), at 28-55 °C (optimum, 28 °C) and in the presence of 0-3 % (w/v) NaCl (optimum in the absence of NaCl).
31125305	3	23	theme	gene	299:302	arg1	sequences					304:312	16S rRNA gene sequences	290:312	16S rRNA gene sequences	290:312	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SYSU K30001T belonged to the genus Bacillus, with the highest sequence similarity to the type strain of Bacillus panaciterrae (98.1 %).
31125305	10	24	theme	molecular	1263:1271	arg1	characterizations					1273:1289	phenotypic, chemotaxonomic and molecular characterizations	1232:1289	phenotypic, chemotaxonomic and molecular characterizations	1232:1289	Based on phenotypic, chemotaxonomic and molecular characterizations, strain SYSU K30001T represents a novel species of the genus Bacillus, for which the name Bacillus antri sp.
31125305	10	25	theme	antri	1390:1394	arg1	sp					1396:1397	Bacillus antri sp	1381:1397	the name Bacillus antri sp	1372:1397	Based on phenotypic, chemotaxonomic and molecular characterizations, strain SYSU K30001T represents a novel species of the genus Bacillus, for which the name Bacillus antri sp.
31125305	0	26	theme	antri	9:13	arg1	sp					15:16	Bacillus antri sp	0:16	Bacillus antri sp.	0:17	Bacillus antri sp.
31125305	8	27	theme	genome	990:995	arg1	G+C content					997:1007	The genome G+C content	986:1007	The genome G+C content	986:1007	The genome G+C content was 39.8 mol%.
31125305	8	27	theme	genome	990:995	arg1	%					1021:1021	39.8 mol%	1013:1021	39.8 mol%	1013:1021	The genome G+C content was 39.8 mol%.
31125305	4	28	from	optimum	591:597	arg1	absence					606:612	the absence	602:612	the absence of NaCl	602:620	Growth occurred at pH 6.0-9.0 (optimum, pH 7.0), at 28-55 °C (optimum, 28 °C) and in the presence of 0-3 % (w/v) NaCl (optimum in the absence of NaCl).
31125305	10	29	theme	Bacillus	1381:1388	arg1	sp					1396:1397	Bacillus antri sp	1381:1397	the name Bacillus antri sp	1372:1397	Based on phenotypic, chemotaxonomic and molecular characterizations, strain SYSU K30001T represents a novel species of the genus Bacillus, for which the name Bacillus antri sp.
31125305	2	30	attach	isolated	155:162	arg1	sample					176:181	a soil sample	169:181	a soil sample collected from a cave in Xingyi county, Guizhou province, south-west China	169:256	A Gram-stain-positive, strictly aerobic, rod-shaped, motile, endospore-forming strain, SYSU K30001T, was isolated from a soil sample collected from a cave in Xingyi county, Guizhou province, south-west China.
31125305	2	30	attach	isolated	155:162	arg2	strain					129:134	A Gram-stain-positive, strictly aerobic, rod-shaped, motile, endospore-forming strain	50:134	A Gram-stain-positive, strictly aerobic, rod-shaped, motile, endospore-forming strain	50:134	A Gram-stain-positive, strictly aerobic, rod-shaped, motile, endospore-forming strain, SYSU K30001T, was isolated from a soil sample collected from a cave in Xingyi county, Guizhou province, south-west China.
31125305	2	30	attach	isolated	155:162	arg2	K30001T					142:148	K30001T	142:148	K30001T	142:148	A Gram-stain-positive, strictly aerobic, rod-shaped, motile, endospore-forming strain, SYSU K30001T, was isolated from a soil sample collected from a cave in Xingyi county, Guizhou province, south-west China.
31125305	0	31	theme	Bacillus	0:7	arg1	sp					15:16	Bacillus antri sp	0:16	Bacillus antri sp.	0:17	Bacillus antri sp.
31125305	10	32	theme	chemotaxonomic	1244:1257	arg1	characterizations					1273:1289	phenotypic, chemotaxonomic and molecular characterizations	1232:1289	phenotypic, chemotaxonomic and molecular characterizations	1232:1289	Based on phenotypic, chemotaxonomic and molecular characterizations, strain SYSU K30001T represents a novel species of the genus Bacillus, for which the name Bacillus antri sp.
31125305	4	33	theme	NaCl	585:588	arg1	presence					561:568	the presence	557:568	the presence of 0-3 % (w/v) NaCl (optimum in the absence of NaCl)	557:621	Growth occurred at pH 6.0-9.0 (optimum, pH 7.0), at 28-55 °C (optimum, 28 °C) and in the presence of 0-3 % (w/v) NaCl (optimum in the absence of NaCl).
31125305	5	34	theme	meso-2,6-diaminopimelic	654:676	arg1	MK-7					718:721	MK-7	718:721	MK-7	718:721	Strain SYSU K30001T contained meso-2,6-diaminopimelic acid in the cell-wall peptidoglycan and MK-7 as the only isoprenoid quinone present.
31125305	5	34	theme	meso-2,6-diaminopimelic	654:676	arg1	present					754:760	the only isoprenoid quinone present	726:760	the only isoprenoid quinone present	726:760	Strain SYSU K30001T contained meso-2,6-diaminopimelic acid in the cell-wall peptidoglycan and MK-7 as the only isoprenoid quinone present.
31125305	5	34	theme	meso-2,6-diaminopimelic	654:676	arg1	acid					678:681	meso-2,6-diaminopimelic acid	654:681	meso-2,6-diaminopimelic acid in the cell-wall peptidoglycan	654:712	Strain SYSU K30001T contained meso-2,6-diaminopimelic acid in the cell-wall peptidoglycan and MK-7 as the only isoprenoid quinone present.
31125305	9	35	theme	average	1028:1034	arg1	values					1056:1061	The average nucleotide identity values	1024:1061	The average nucleotide identity values between SYSU K30001T and B. panaciterrae DSM 19096T	1024:1113	The average nucleotide identity values between SYSU K30001T and B. panaciterrae DSM 19096T were 72.1 % (ANIb) and 83.1 % (ANIm), which were below the cut-off level (95-96 %) for species delineation.
31125305	9	35	theme	average	1028:1034	arg1	%					1125:1125	72.1 %	1120:1125	72.1 % (ANIb)	1120:1132	The average nucleotide identity values between SYSU K30001T and B. panaciterrae DSM 19096T were 72.1 % (ANIb) and 83.1 % (ANIm), which were below the cut-off level (95-96 %) for species delineation.
31125305	2	36	from	cave	200:203	arg1	province					231:238	province	231:238	province	231:238	A Gram-stain-positive, strictly aerobic, rod-shaped, motile, endospore-forming strain, SYSU K30001T, was isolated from a soil sample collected from a cave in Xingyi county, Guizhou province, south-west China.
31125305	2	36	from	cave	200:203	arg1	China					252:256	south-west China	241:256	south-west China	241:256	A Gram-stain-positive, strictly aerobic, rod-shaped, motile, endospore-forming strain, SYSU K30001T, was isolated from a soil sample collected from a cave in Xingyi county, Guizhou province, south-west China.
31125305	2	36	from	cave	200:203	arg1	county					215:220	county	215:220	county	215:220	A Gram-stain-positive, strictly aerobic, rod-shaped, motile, endospore-forming strain, SYSU K30001T, was isolated from a soil sample collected from a cave in Xingyi county, Guizhou province, south-west China.
31125305	3	37	theme	strain	329:334	arg1	K30001T					341:347	strain SYSU K30001T	329:347	strain SYSU K30001T	329:347	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SYSU K30001T belonged to the genus Bacillus, with the highest sequence similarity to the type strain of Bacillus panaciterrae (98.1 %).
31125305	10	38	theme	SYSU	1299:1302	arg1	K30001T					1304:1310	strain SYSU K30001T	1292:1310	strain SYSU K30001T	1292:1310	Based on phenotypic, chemotaxonomic and molecular characterizations, strain SYSU K30001T represents a novel species of the genus Bacillus, for which the name Bacillus antri sp.
31125305	2	39	theme	south-west	241:250	arg1	China					252:256	south-west China	241:256	south-west China	241:256	A Gram-stain-positive, strictly aerobic, rod-shaped, motile, endospore-forming strain, SYSU K30001T, was isolated from a soil sample collected from a cave in Xingyi county, Guizhou province, south-west China.
31125305	2	39	theme	south-west	241:250	arg1	county					215:220	county	215:220	county	215:220	A Gram-stain-positive, strictly aerobic, rod-shaped, motile, endospore-forming strain, SYSU K30001T, was isolated from a soil sample collected from a cave in Xingyi county, Guizhou province, south-west China.
31125305	10	40	theme	strain	1292:1297	arg1	K30001T					1304:1310	strain SYSU K30001T	1292:1310	strain SYSU K30001T	1292:1310	Based on phenotypic, chemotaxonomic and molecular characterizations, strain SYSU K30001T represents a novel species of the genus Bacillus, for which the name Bacillus antri sp.
31125305	5	41	theme	cell-wall	690:698	arg1	peptidoglycan					700:712	the cell-wall peptidoglycan	686:712	the cell-wall peptidoglycan	686:712	Strain SYSU K30001T contained meso-2,6-diaminopimelic acid in the cell-wall peptidoglycan and MK-7 as the only isoprenoid quinone present.
31125305	12	42	theme	type	1422:1425	arg1	strain					1427:1432	The type strain	1418:1432	The type strain	1418:1432	The type strain is SYSU K30001T (=KCTC 33954T=CGMCC 1.13871T).
31125305	12	42	theme	type	1422:1425	arg1	K30001T					1442:1448	SYSU K30001T	1437:1448	SYSU K30001T (=KCTC 33954T=CGMCC 1.13871T)	1437:1478	The type strain is SYSU K30001T (=KCTC 33954T=CGMCC 1.13871T).
31125305	3	43	theme	highest	390:396	arg1	similarity					407:416	the highest sequence similarity	386:416	the highest sequence similarity to the type strain of Bacillus panaciterrae (98.1 %)	386:469	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SYSU K30001T belonged to the genus Bacillus, with the highest sequence similarity to the type strain of Bacillus panaciterrae (98.1 %).
31125305	4	44	dep	optimum	503:509	arg1	pH					512:513	pH 7.0	512:517	pH 7.0	512:517	Growth occurred at pH 6.0-9.0 (optimum, pH 7.0), at 28-55 °C (optimum, 28 °C) and in the presence of 0-3 % (w/v) NaCl (optimum in the absence of NaCl).
31125305	4	45	dep	optimum	534:540	arg1	28 °C					543:547	28 °C	543:547	28 °C	543:547	Growth occurred at pH 6.0-9.0 (optimum, pH 7.0), at 28-55 °C (optimum, 28 °C) and in the presence of 0-3 % (w/v) NaCl (optimum in the absence of NaCl).
31125305	7	46	theme	fatty	906:910	arg1	iso-C15 					923:930	iso-C15 	923:930	iso-C15 	923:930	The major fatty acids were iso-C15 : 0, iso-C17 : 1ω10c, anteiso-C14 : 0 and iso-C17 : 0.
31125305	7	46	theme	fatty	906:910	arg1	acids					912:916	The major fatty acids	896:916	The major fatty acids	896:916	The major fatty acids were iso-C15 : 0, iso-C17 : 1ω10c, anteiso-C14 : 0 and iso-C17 : 0.
31125305	3	47	theme	sequence	398:405	arg1	similarity					407:416	the highest sequence similarity	386:416	the highest sequence similarity to the type strain of Bacillus panaciterrae (98.1 %)	386:469	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SYSU K30001T belonged to the genus Bacillus, with the highest sequence similarity to the type strain of Bacillus panaciterrae (98.1 %).
31125305	9	48	theme	DSM	1104:1106	arg1	19096T					1108:1113	DSM 19096T	1104:1113	DSM 19096T	1104:1113	The average nucleotide identity values between SYSU K30001T and B. panaciterrae DSM 19096T were 72.1 % (ANIb) and 83.1 % (ANIm), which were below the cut-off level (95-96 %) for species delineation.
31125305	10	49	theme	novel	1325:1329	arg1	species					1331:1337	a novel species	1323:1337	a novel species	1323:1337	Based on phenotypic, chemotaxonomic and molecular characterizations, strain SYSU K30001T represents a novel species of the genus Bacillus, for which the name Bacillus antri sp.
31125305	9	50	theme	cut-off	1174:1180	arg1	%					1195:1195	95-96 %	1189:1195	95-96 %	1189:1195	The average nucleotide identity values between SYSU K30001T and B. panaciterrae DSM 19096T were 72.1 % (ANIb) and 83.1 % (ANIm), which were below the cut-off level (95-96 %) for species delineation.
31125305	9	50	theme	cut-off	1174:1180	arg1	level					1182:1186	the cut-off level	1170:1186	the cut-off level (95-96 %)	1170:1196	The average nucleotide identity values between SYSU K30001T and B. panaciterrae DSM 19096T were 72.1 % (ANIb) and 83.1 % (ANIm), which were below the cut-off level (95-96 %) for species delineation.
31125305	3	51	theme	Phylogenetic	259:270	arg1	analysis					272:279	Phylogenetic analysis	259:279	Phylogenetic analysis based on 16S rRNA gene sequences	259:312	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SYSU K30001T belonged to the genus Bacillus, with the highest sequence similarity to the type strain of Bacillus panaciterrae (98.1 %).
31125305	2	52	theme	soil	171:174	arg1	sample					176:181	a soil sample	169:181	a soil sample collected from a cave in Xingyi county, Guizhou province, south-west China	169:256	A Gram-stain-positive, strictly aerobic, rod-shaped, motile, endospore-forming strain, SYSU K30001T, was isolated from a soil sample collected from a cave in Xingyi county, Guizhou province, south-west China.
31125305	10	53	dep	name	1376:1379	arg1	sp					1396:1397	Bacillus antri sp	1381:1397	the name Bacillus antri sp	1372:1397	Based on phenotypic, chemotaxonomic and molecular characterizations, strain SYSU K30001T represents a novel species of the genus Bacillus, for which the name Bacillus antri sp.
31125305	4	54	theme	%	577:577	arg1	NaCl					585:588	0-3 % (w/v) NaCl	573:588	0-3 % (w/v) NaCl (optimum in the absence of NaCl)	573:621	Growth occurred at pH 6.0-9.0 (optimum, pH 7.0), at 28-55 °C (optimum, 28 °C) and in the presence of 0-3 % (w/v) NaCl (optimum in the absence of NaCl).
31125305	2	55	theme	Gram-stain-positive	52:70	arg1	strain					129:134	A Gram-stain-positive, strictly aerobic, rod-shaped, motile, endospore-forming strain	50:134	A Gram-stain-positive, strictly aerobic, rod-shaped, motile, endospore-forming strain	50:134	A Gram-stain-positive, strictly aerobic, rod-shaped, motile, endospore-forming strain, SYSU K30001T, was isolated from a soil sample collected from a cave in Xingyi county, Guizhou province, south-west China.
31125305	2	55	theme	Gram-stain-positive	52:70	arg1	K30001T					142:148	K30001T	142:148	K30001T	142:148	A Gram-stain-positive, strictly aerobic, rod-shaped, motile, endospore-forming strain, SYSU K30001T, was isolated from a soil sample collected from a cave in Xingyi county, Guizhou province, south-west China.
31125305	7	56	theme	major	900:904	arg1	iso-C15 					923:930	iso-C15 	923:930	iso-C15 	923:930	The major fatty acids were iso-C15 : 0, iso-C17 : 1ω10c, anteiso-C14 : 0 and iso-C17 : 0.
31125305	7	56	theme	major	900:904	arg1	acids					912:916	The major fatty acids	896:916	The major fatty acids	896:916	The major fatty acids were iso-C15 : 0, iso-C17 : 1ω10c, anteiso-C14 : 0 and iso-C17 : 0.
31125305	12	57	theme	=KCTC	1451:1455	arg1	1.13871T					1470:1477	=KCTC 33954T=CGMCC 1.13871T	1451:1477	=KCTC 33954T=CGMCC 1.13871T	1451:1477	The type strain is SYSU K30001T (=KCTC 33954T=CGMCC 1.13871T).
31125305	12	57	theme	=KCTC	1451:1455	arg1	K30001T					1442:1448	SYSU K30001T	1437:1448	SYSU K30001T (=KCTC 33954T=CGMCC 1.13871T)	1437:1478	The type strain is SYSU K30001T (=KCTC 33954T=CGMCC 1.13871T).
31125305	3	58	theme	type	425:428	arg1	strain					430:435	the type strain	421:435	the type strain of Bacillus panaciterrae (98.1 %)	421:469	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SYSU K30001T belonged to the genus Bacillus, with the highest sequence similarity to the type strain of Bacillus panaciterrae (98.1 %).
31125305	7	59	dep	 0	932:933	arg1	 1ω10c					945:950	 1ω10c	945:950	 1ω10c	945:950	The major fatty acids were iso-C15 : 0, iso-C17 : 1ω10c, anteiso-C14 : 0 and iso-C17 : 0.
31125305	10	60	theme	Bacillus	1352:1359	arg1	species					1331:1337	a novel species	1323:1337	a novel species	1323:1337	Based on phenotypic, chemotaxonomic and molecular characterizations, strain SYSU K30001T represents a novel species of the genus Bacillus, for which the name Bacillus antri sp.
31125305	5	61	theme	isoprenoid	735:744	arg1	MK-7					718:721	MK-7	718:721	MK-7	718:721	Strain SYSU K30001T contained meso-2,6-diaminopimelic acid in the cell-wall peptidoglycan and MK-7 as the only isoprenoid quinone present.
31125305	5	61	theme	isoprenoid	735:744	arg1	present					754:760	the only isoprenoid quinone present	726:760	the only isoprenoid quinone present	726:760	Strain SYSU K30001T contained meso-2,6-diaminopimelic acid in the cell-wall peptidoglycan and MK-7 as the only isoprenoid quinone present.
31125305	5	61	theme	isoprenoid	735:744	arg1	acid					678:681	meso-2,6-diaminopimelic acid	654:681	meso-2,6-diaminopimelic acid in the cell-wall peptidoglycan	654:712	Strain SYSU K30001T contained meso-2,6-diaminopimelic acid in the cell-wall peptidoglycan and MK-7 as the only isoprenoid quinone present.
31125305	3	62	theme	16S	290:292	arg1	sequences					304:312	16S rRNA gene sequences	290:312	16S rRNA gene sequences	290:312	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SYSU K30001T belonged to the genus Bacillus, with the highest sequence similarity to the type strain of Bacillus panaciterrae (98.1 %).
31125305	4	63	dep	NaCl	585:588	arg1	optimum					591:597	optimum	591:597	optimum	591:597	Growth occurred at pH 6.0-9.0 (optimum, pH 7.0), at 28-55 °C (optimum, 28 °C) and in the presence of 0-3 % (w/v) NaCl (optimum in the absence of NaCl).
31125305	3	64	theme	SYSU	336:339	arg1	K30001T					341:347	strain SYSU K30001T	329:347	strain SYSU K30001T	329:347	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SYSU K30001T belonged to the genus Bacillus, with the highest sequence similarity to the type strain of Bacillus panaciterrae (98.1 %).
31125305	12	65	theme	33954T=CGMCC	1457:1468	arg1	1.13871T					1470:1477	=KCTC 33954T=CGMCC 1.13871T	1451:1477	=KCTC 33954T=CGMCC 1.13871T	1451:1477	The type strain is SYSU K30001T (=KCTC 33954T=CGMCC 1.13871T).
31125305	12	65	theme	33954T=CGMCC	1457:1468	arg1	K30001T					1442:1448	SYSU K30001T	1437:1448	SYSU K30001T (=KCTC 33954T=CGMCC 1.13871T)	1437:1478	The type strain is SYSU K30001T (=KCTC 33954T=CGMCC 1.13871T).
31125305	9	66	dep	B.	1088:1089	arg1	panaciterrae					1091:1102	B. panaciterrae	1088:1102	B. panaciterrae	1088:1102	The average nucleotide identity values between SYSU K30001T and B. panaciterrae DSM 19096T were 72.1 % (ANIb) and 83.1 % (ANIm), which were below the cut-off level (95-96 %) for species delineation.
30562014	2	0	theme	GOS	520:522	arg1	group					524:528	the GOS group	516:528	the GOS group	516:528	Ten milliliter 1 g/kg body weight GOS solutions and physiological saline solutions were fed to the newborn piglets in the GOS group and in the control (CON) group a week time, respectively.
30562014	4	1	theme	higher	671:676	arg1	abundance					678:686	a higher abundance	669:686	a higher abundance of short-chain fatty acids (SCFAs) producer such as Prevotella, Barnesiella, Parabacteroides, and Unclassified Porphyromonadaceae in colonic mucosa ( P < 0.05)	669:846	GOS piglets had a higher abundance of short-chain fatty acids (SCFAs) producer such as Prevotella, Barnesiella, Parabacteroides, and Unclassified Porphyromonadaceae in colonic mucosa ( P < 0.05).
30562014	2	2	from	time	568:571	arg1	group					524:528	the GOS group	516:528	the GOS group	516:528	Ten milliliter 1 g/kg body weight GOS solutions and physiological saline solutions were fed to the newborn piglets in the GOS group and in the control (CON) group a week time, respectively.
30562014	5	3	theme	total	866:870	arg1	level					878:882	the total SCFAs level	862:882	the total SCFAs level in colonic digesta of GOS piglets	862:916	In addition, the total SCFAs level in colonic digesta of GOS piglets increased on day 8 ( P < 0.05) and day 21 ( P = 0.064).
30562014	7	4	theme	mucosal	1345:1351	arg1	colonization					1364:1375	the mucosal microbiota colonization	1341:1375	the mucosal microbiota colonization	1341:1375	In summary, these results provide important insights and understandings to reveal the relationship between the mucosal microbiota colonization and intestinal function at the early life stage of piglets.
30562014	4	5	theme	P	838:838	arg1	mucosa					829:834	colonic mucosa	821:834	colonic mucosa ( P < 0.05)	821:846	GOS piglets had a higher abundance of short-chain fatty acids (SCFAs) producer such as Prevotella, Barnesiella, Parabacteroides, and Unclassified Porphyromonadaceae in colonic mucosa ( P < 0.05).
30562014	4	5	theme	P	838:838	arg1	<					840:840	P < 0.05	838:845	P < 0.05	838:845	GOS piglets had a higher abundance of short-chain fatty acids (SCFAs) producer such as Prevotella, Barnesiella, Parabacteroides, and Unclassified Porphyromonadaceae in colonic mucosa ( P < 0.05).
30562014	6	6	theme	signaling	1215:1223	arg1	pathway					1225:1231	the NFκB and AMPK signaling pathway	1197:1231	pathway	1225:1231	Meanwhile, a higher SCFAs concentration in colon of the GOS piglets altered the gene expression of inflammatory cytokines (IL-8 and IL-10) and barrier proteins (ZO-1 and Claudin-1) through regulating the phosphorylation of the NFκB and AMPK signaling pathway.
30562014	1	7	theme	Suckling	183:190	arg1	piglets					192:198	Suckling piglets	183:198	Suckling piglets	183:198	Suckling piglets were used to investigate the response of colonic mucosa-associated microbiota composition, mucosal immune homeostasis, and barrier function to early life galactooligosaccharides (GOS) intervention.
30562014	1	8	theme	mucosal	291:297	arg1	homeostasis					306:316	mucosal immune homeostasis	291:316	mucosal immune homeostasis	291:316	Suckling piglets were used to investigate the response of colonic mucosa-associated microbiota composition, mucosal immune homeostasis, and barrier function to early life galactooligosaccharides (GOS) intervention.
30562014	0	9	theme	Function	102:109	arg1	Response					0:7	Response	0:7	Response of Colonic Mucosa-Associated Microbiota Composition, Mucosal Immune Homeostasis, and Barrier Function to Early Life	0:123	Response of Colonic Mucosa-Associated Microbiota Composition, Mucosal Immune Homeostasis, and Barrier Function to Early Life Galactooligosaccharides Intervention in Suckling Piglets.
30562014	4	10	contain	had	665:667	arg1	piglets					657:663	GOS piglets	653:663	GOS piglets	653:663	GOS piglets had a higher abundance of short-chain fatty acids (SCFAs) producer such as Prevotella, Barnesiella, Parabacteroides, and Unclassified Porphyromonadaceae in colonic mucosa ( P < 0.05).
30562014	4	10	contain	had	665:667	arg2	abundance					678:686	a higher abundance	669:686	a higher abundance of short-chain fatty acids (SCFAs) producer such as Prevotella, Barnesiella, Parabacteroides, and Unclassified Porphyromonadaceae in colonic mucosa ( P < 0.05)	669:846	GOS piglets had a higher abundance of short-chain fatty acids (SCFAs) producer such as Prevotella, Barnesiella, Parabacteroides, and Unclassified Porphyromonadaceae in colonic mucosa ( P < 0.05).
30562014	6	11	from	concentration	1000:1012	arg1	colon					1017:1021	colon	1017:1021	colon of the GOS piglets	1017:1040	Meanwhile, a higher SCFAs concentration in colon of the GOS piglets altered the gene expression of inflammatory cytokines (IL-8 and IL-10) and barrier proteins (ZO-1 and Claudin-1) through regulating the phosphorylation of the NFκB and AMPK signaling pathway.
30562014	5	12	theme	GOS	906:908	arg1	piglets					910:916	GOS piglets	906:916	GOS piglets	906:916	In addition, the total SCFAs level in colonic digesta of GOS piglets increased on day 8 ( P < 0.05) and day 21 ( P = 0.064).
30562014	1	13	theme	immune	299:304	arg1	homeostasis					306:316	mucosal immune homeostasis	291:316	mucosal immune homeostasis	291:316	Suckling piglets were used to investigate the response of colonic mucosa-associated microbiota composition, mucosal immune homeostasis, and barrier function to early life galactooligosaccharides (GOS) intervention.
30562014	0	14	theme	Barrier	94:100	arg1	Function					102:109	Barrier Function	94:109	Barrier Function	94:109	Response of Colonic Mucosa-Associated Microbiota Composition, Mucosal Immune Homeostasis, and Barrier Function to Early Life Galactooligosaccharides Intervention in Suckling Piglets.
30562014	6	15	theme	GOS	1030:1032	arg1	piglets					1034:1040	the GOS piglets	1026:1040	the GOS piglets	1026:1040	Meanwhile, a higher SCFAs concentration in colon of the GOS piglets altered the gene expression of inflammatory cytokines (IL-8 and IL-10) and barrier proteins (ZO-1 and Claudin-1) through regulating the phosphorylation of the NFκB and AMPK signaling pathway.
30562014	6	16	theme	gene	1054:1057	arg1	expression					1059:1068	the gene expression	1050:1068	the gene expression of inflammatory cytokines (IL-8 and IL-10) and barrier proteins (ZO-1 and Claudin-1)	1050:1153	Meanwhile, a higher SCFAs concentration in colon of the GOS piglets altered the gene expression of inflammatory cytokines (IL-8 and IL-10) and barrier proteins (ZO-1 and Claudin-1) through regulating the phosphorylation of the NFκB and AMPK signaling pathway.
30562014	6	17	theme	cytokines	1086:1094	arg1	expression					1059:1068	the gene expression	1050:1068	the gene expression of inflammatory cytokines (IL-8 and IL-10) and barrier proteins (ZO-1 and Claudin-1)	1050:1153	Meanwhile, a higher SCFAs concentration in colon of the GOS piglets altered the gene expression of inflammatory cytokines (IL-8 and IL-10) and barrier proteins (ZO-1 and Claudin-1) through regulating the phosphorylation of the NFκB and AMPK signaling pathway.
30562014	5	18	theme	piglets	910:916	arg1	digesta					895:901	colonic digesta	887:901	colonic digesta of GOS piglets	887:916	In addition, the total SCFAs level in colonic digesta of GOS piglets increased on day 8 ( P < 0.05) and day 21 ( P = 0.064).
30562014	1	19	theme	homeostasis	306:316	arg1	response					229:236	the response	225:236	the response of colonic mucosa-associated microbiota composition, mucosal immune homeostasis, and barrier function to early life galactooligosaccharides (GOS) intervention	225:395	Suckling piglets were used to investigate the response of colonic mucosa-associated microbiota composition, mucosal immune homeostasis, and barrier function to early life galactooligosaccharides (GOS) intervention.
30562014	0	20	theme	Early	114:118	arg1	Life					120:123	Early Life	114:123	Early Life	114:123	Response of Colonic Mucosa-Associated Microbiota Composition, Mucosal Immune Homeostasis, and Barrier Function to Early Life Galactooligosaccharides Intervention in Suckling Piglets.
30562014	4	21	theme	colonic	821:827	arg1	mucosa					829:834	colonic mucosa	821:834	colonic mucosa ( P < 0.05)	821:846	GOS piglets had a higher abundance of short-chain fatty acids (SCFAs) producer such as Prevotella, Barnesiella, Parabacteroides, and Unclassified Porphyromonadaceae in colonic mucosa ( P < 0.05).
30562014	4	21	theme	colonic	821:827	arg1	<					840:840	P < 0.05	838:845	P < 0.05	838:845	GOS piglets had a higher abundance of short-chain fatty acids (SCFAs) producer such as Prevotella, Barnesiella, Parabacteroides, and Unclassified Porphyromonadaceae in colonic mucosa ( P < 0.05).
30562014	3	22	from	group	610:614	arg1	piglets					592:598	Six piglets	588:598	Six piglets from each group	588:614	Six piglets from each group were euthanized on day 8 and day 21.
30562014	2	23	theme	GOS	432:434	arg1	solutions					436:444	Ten milliliter 1 g/kg body weight GOS solutions	398:444	Ten milliliter 1 g/kg body weight GOS solutions	398:444	Ten milliliter 1 g/kg body weight GOS solutions and physiological saline solutions were fed to the newborn piglets in the GOS group and in the control (CON) group a week time, respectively.
30562014	6	24	theme	AMPK	1210:1213	arg1	pathway					1225:1231	the NFκB and AMPK signaling pathway	1197:1231	pathway	1225:1231	Meanwhile, a higher SCFAs concentration in colon of the GOS piglets altered the gene expression of inflammatory cytokines (IL-8 and IL-10) and barrier proteins (ZO-1 and Claudin-1) through regulating the phosphorylation of the NFκB and AMPK signaling pathway.
30562014	6	25	theme	proteins	1125:1132	arg1	expression					1059:1068	the gene expression	1050:1068	the gene expression of inflammatory cytokines (IL-8 and IL-10) and barrier proteins (ZO-1 and Claudin-1)	1050:1153	Meanwhile, a higher SCFAs concentration in colon of the GOS piglets altered the gene expression of inflammatory cytokines (IL-8 and IL-10) and barrier proteins (ZO-1 and Claudin-1) through regulating the phosphorylation of the NFκB and AMPK signaling pathway.
30562014	2	26	theme	weight	425:430	arg1	solutions					436:444	Ten milliliter 1 g/kg body weight GOS solutions	398:444	Ten milliliter 1 g/kg body weight GOS solutions	398:444	Ten milliliter 1 g/kg body weight GOS solutions and physiological saline solutions were fed to the newborn piglets in the GOS group and in the control (CON) group a week time, respectively.
30562014	6	27	dep	proteins	1125:1132	arg1	proteins					1125:1132	barrier proteins	1117:1132	barrier proteins (ZO-1 and Claudin-1)	1117:1153	Meanwhile, a higher SCFAs concentration in colon of the GOS piglets altered the gene expression of inflammatory cytokines (IL-8 and IL-10) and barrier proteins (ZO-1 and Claudin-1) through regulating the phosphorylation of the NFκB and AMPK signaling pathway.
30562014	6	27	dep	proteins	1125:1132	arg1	Claudin-1					1144:1152	Claudin-1	1144:1152	Claudin-1	1144:1152	Meanwhile, a higher SCFAs concentration in colon of the GOS piglets altered the gene expression of inflammatory cytokines (IL-8 and IL-10) and barrier proteins (ZO-1 and Claudin-1) through regulating the phosphorylation of the NFκB and AMPK signaling pathway.
30562014	6	27	dep	proteins	1125:1132	arg1	ZO-1					1135:1138	ZO-1	1135:1138	ZO-1	1135:1138	Meanwhile, a higher SCFAs concentration in colon of the GOS piglets altered the gene expression of inflammatory cytokines (IL-8 and IL-10) and barrier proteins (ZO-1 and Claudin-1) through regulating the phosphorylation of the NFκB and AMPK signaling pathway.
30562014	6	28	theme	barrier	1117:1123	arg1	proteins					1125:1132	barrier proteins	1117:1132	barrier proteins (ZO-1 and Claudin-1)	1117:1153	Meanwhile, a higher SCFAs concentration in colon of the GOS piglets altered the gene expression of inflammatory cytokines (IL-8 and IL-10) and barrier proteins (ZO-1 and Claudin-1) through regulating the phosphorylation of the NFκB and AMPK signaling pathway.
30562014	6	28	theme	barrier	1117:1123	arg1	Claudin-1					1144:1152	Claudin-1	1144:1152	Claudin-1	1144:1152	Meanwhile, a higher SCFAs concentration in colon of the GOS piglets altered the gene expression of inflammatory cytokines (IL-8 and IL-10) and barrier proteins (ZO-1 and Claudin-1) through regulating the phosphorylation of the NFκB and AMPK signaling pathway.
30562014	6	28	theme	barrier	1117:1123	arg1	ZO-1					1135:1138	ZO-1	1135:1138	ZO-1	1135:1138	Meanwhile, a higher SCFAs concentration in colon of the GOS piglets altered the gene expression of inflammatory cytokines (IL-8 and IL-10) and barrier proteins (ZO-1 and Claudin-1) through regulating the phosphorylation of the NFκB and AMPK signaling pathway.
30562014	5	29	from	level	878:882	arg1	digesta					895:901	colonic digesta	887:901	colonic digesta of GOS piglets	887:916	In addition, the total SCFAs level in colonic digesta of GOS piglets increased on day 8 ( P < 0.05) and day 21 ( P = 0.064).
30562014	6	30	theme	piglets	1034:1040	arg1	colon					1017:1021	colon	1017:1021	colon of the GOS piglets	1017:1040	Meanwhile, a higher SCFAs concentration in colon of the GOS piglets altered the gene expression of inflammatory cytokines (IL-8 and IL-10) and barrier proteins (ZO-1 and Claudin-1) through regulating the phosphorylation of the NFκB and AMPK signaling pathway.
30562014	2	31	theme	body	420:423	arg1	weight					425:430	Ten milliliter 1 g/kg body weight	398:430	Ten milliliter 1 g/kg body weight GOS solutions	398:444	Ten milliliter 1 g/kg body weight GOS solutions and physiological saline solutions were fed to the newborn piglets in the GOS group and in the control (CON) group a week time, respectively.
30562014	4	32	theme	acids	709:713	arg1	abundance					678:686	a higher abundance	669:686	a higher abundance of short-chain fatty acids (SCFAs) producer such as Prevotella, Barnesiella, Parabacteroides, and Unclassified Porphyromonadaceae in colonic mucosa ( P < 0.05)	669:846	GOS piglets had a higher abundance of short-chain fatty acids (SCFAs) producer such as Prevotella, Barnesiella, Parabacteroides, and Unclassified Porphyromonadaceae in colonic mucosa ( P < 0.05).
30562014	6	33	theme	NFκB	1201:1204	arg1	phosphorylation					1178:1192	the phosphorylation	1174:1192	the phosphorylation of the NFκB and AMPK signaling pathway	1174:1231	Meanwhile, a higher SCFAs concentration in colon of the GOS piglets altered the gene expression of inflammatory cytokines (IL-8 and IL-10) and barrier proteins (ZO-1 and Claudin-1) through regulating the phosphorylation of the NFκB and AMPK signaling pathway.
30562014	2	34	theme	g/kg	415:418	arg1	weight					425:430	Ten milliliter 1 g/kg body weight	398:430	Ten milliliter 1 g/kg body weight GOS solutions	398:444	Ten milliliter 1 g/kg body weight GOS solutions and physiological saline solutions were fed to the newborn piglets in the GOS group and in the control (CON) group a week time, respectively.
30562014	0	35	theme	Mucosa-Associated	20:36	arg1	Composition					49:59	Colonic Mucosa-Associated Microbiota Composition	12:59	Colonic Mucosa-Associated Microbiota Composition	12:59	Response of Colonic Mucosa-Associated Microbiota Composition, Mucosal Immune Homeostasis, and Barrier Function to Early Life Galactooligosaccharides Intervention in Suckling Piglets.
30562014	7	36	theme	important	1268:1276	arg1	insights					1278:1285	important insights	1268:1285	important insights	1268:1285	In summary, these results provide important insights and understandings to reveal the relationship between the mucosal microbiota colonization and intestinal function at the early life stage of piglets.
30562014	2	37	theme	saline	464:469	arg1	solutions					471:479	physiological saline solutions	450:479	physiological saline solutions	450:479	Ten milliliter 1 g/kg body weight GOS solutions and physiological saline solutions were fed to the newborn piglets in the GOS group and in the control (CON) group a week time, respectively.
30562014	0	38	theme	Colonic	12:18	arg1	Composition					49:59	Colonic Mucosa-Associated Microbiota Composition	12:59	Colonic Mucosa-Associated Microbiota Composition	12:59	Response of Colonic Mucosa-Associated Microbiota Composition, Mucosal Immune Homeostasis, and Barrier Function to Early Life Galactooligosaccharides Intervention in Suckling Piglets.
30562014	1	39	theme	barrier	323:329	arg1	function					331:338	barrier function	323:338	barrier function	323:338	Suckling piglets were used to investigate the response of colonic mucosa-associated microbiota composition, mucosal immune homeostasis, and barrier function to early life galactooligosaccharides (GOS) intervention.
30562014	5	40	theme	colonic	887:893	arg1	digesta					895:901	colonic digesta	887:901	colonic digesta of GOS piglets	887:916	In addition, the total SCFAs level in colonic digesta of GOS piglets increased on day 8 ( P < 0.05) and day 21 ( P = 0.064).
30562014	5	41	theme	=	964:964	arg1	day					953:955	day 21	953:958	day 21 ( P = 0.064)	953:971	In addition, the total SCFAs level in colonic digesta of GOS piglets increased on day 8 ( P < 0.05) and day 21 ( P = 0.064).
30562014	5	41	theme	=	964:964	arg1	P					962:962	P = 0.064	962:970	P = 0.064	962:970	In addition, the total SCFAs level in colonic digesta of GOS piglets increased on day 8 ( P < 0.05) and day 21 ( P = 0.064).
30562014	4	42	dep	acids	709:713	arg1	producer					723:730	producer	723:730	short-chain fatty acids (SCFAs) producer such as Prevotella, Barnesiella, Parabacteroides, and Unclassified Porphyromonadaceae in colonic mucosa ( P < 0.05)	691:846	GOS piglets had a higher abundance of short-chain fatty acids (SCFAs) producer such as Prevotella, Barnesiella, Parabacteroides, and Unclassified Porphyromonadaceae in colonic mucosa ( P < 0.05).
30562014	2	43	theme	newborn	497:503	arg1	piglets					505:511	the newborn piglets	493:511	the newborn piglets	493:511	Ten milliliter 1 g/kg body weight GOS solutions and physiological saline solutions were fed to the newborn piglets in the GOS group and in the control (CON) group a week time, respectively.
30562014	2	44	theme	physiological	450:462	arg1	solutions					471:479	physiological saline solutions	450:479	physiological saline solutions	450:479	Ten milliliter 1 g/kg body weight GOS solutions and physiological saline solutions were fed to the newborn piglets in the GOS group and in the control (CON) group a week time, respectively.
30562014	0	45	theme	Composition	49:59	arg1	Response					0:7	Response	0:7	Response of Colonic Mucosa-Associated Microbiota Composition, Mucosal Immune Homeostasis, and Barrier Function to Early Life	0:123	Response of Colonic Mucosa-Associated Microbiota Composition, Mucosal Immune Homeostasis, and Barrier Function to Early Life Galactooligosaccharides Intervention in Suckling Piglets.
30562014	1	46	theme	function	331:338	arg1	response					229:236	the response	225:236	the response of colonic mucosa-associated microbiota composition, mucosal immune homeostasis, and barrier function to early life galactooligosaccharides (GOS) intervention	225:395	Suckling piglets were used to investigate the response of colonic mucosa-associated microbiota composition, mucosal immune homeostasis, and barrier function to early life galactooligosaccharides (GOS) intervention.
30562014	5	47	theme	P	939:939	arg1	day					931:933	day 8	931:935	day 8 ( P < 0.05)	931:947	In addition, the total SCFAs level in colonic digesta of GOS piglets increased on day 8 ( P < 0.05) and day 21 ( P = 0.064).
30562014	5	47	theme	P	939:939	arg1	<					941:941	P < 0.05	939:946	P < 0.05	939:946	In addition, the total SCFAs level in colonic digesta of GOS piglets increased on day 8 ( P < 0.05) and day 21 ( P = 0.064).
30562014	4	48	theme	GOS	653:655	arg1	piglets					657:663	GOS piglets	653:663	GOS piglets	653:663	GOS piglets had a higher abundance of short-chain fatty acids (SCFAs) producer such as Prevotella, Barnesiella, Parabacteroides, and Unclassified Porphyromonadaceae in colonic mucosa ( P < 0.05).
30562014	0	49	theme	Microbiota	38:47	arg1	Composition					49:59	Colonic Mucosa-Associated Microbiota Composition	12:59	Colonic Mucosa-Associated Microbiota Composition	12:59	Response of Colonic Mucosa-Associated Microbiota Composition, Mucosal Immune Homeostasis, and Barrier Function to Early Life Galactooligosaccharides Intervention in Suckling Piglets.
30562014	2	50	theme	CON	550:552	arg1	group					555:559	the control (CON) group	537:559	the control (CON) group	537:559	Ten milliliter 1 g/kg body weight GOS solutions and physiological saline solutions were fed to the newborn piglets in the GOS group and in the control (CON) group a week time, respectively.
30562014	7	51	theme	early	1408:1412	arg1	stage					1419:1423	the early life stage	1404:1423	the early life stage of piglets	1404:1434	In summary, these results provide important insights and understandings to reveal the relationship between the mucosal microbiota colonization and intestinal function at the early life stage of piglets.
30562014	4	52	from	Porphyromonadaceae	799:816	arg1	mucosa					829:834	colonic mucosa	821:834	colonic mucosa ( P < 0.05)	821:846	GOS piglets had a higher abundance of short-chain fatty acids (SCFAs) producer such as Prevotella, Barnesiella, Parabacteroides, and Unclassified Porphyromonadaceae in colonic mucosa ( P < 0.05).
30562014	4	52	from	Porphyromonadaceae	799:816	arg1	<					840:840	P < 0.05	838:845	P < 0.05	838:845	GOS piglets had a higher abundance of short-chain fatty acids (SCFAs) producer such as Prevotella, Barnesiella, Parabacteroides, and Unclassified Porphyromonadaceae in colonic mucosa ( P < 0.05).
30562014	0	53	theme	Mucosal	62:68	arg1	Homeostasis					77:87	Mucosal Immune Homeostasis	62:87	Mucosal Immune Homeostasis	62:87	Response of Colonic Mucosa-Associated Microbiota Composition, Mucosal Immune Homeostasis, and Barrier Function to Early Life Galactooligosaccharides Intervention in Suckling Piglets.
30562014	1	54	theme	early	343:347	arg1	intervention					384:395	early life galactooligosaccharides (GOS) intervention	343:395	early life galactooligosaccharides (GOS) intervention	343:395	Suckling piglets were used to investigate the response of colonic mucosa-associated microbiota composition, mucosal immune homeostasis, and barrier function to early life galactooligosaccharides (GOS) intervention.
30562014	7	55	theme	life	1414:1417	arg1	stage					1419:1423	the early life stage	1404:1423	the early life stage of piglets	1404:1434	In summary, these results provide important insights and understandings to reveal the relationship between the mucosal microbiota colonization and intestinal function at the early life stage of piglets.
30562014	2	56	theme	control	541:547	arg1	group					555:559	the control (CON) group	537:559	the control (CON) group	537:559	Ten milliliter 1 g/kg body weight GOS solutions and physiological saline solutions were fed to the newborn piglets in the GOS group and in the control (CON) group a week time, respectively.
30562014	7	57	theme	piglets	1428:1434	arg1	stage					1419:1423	the early life stage	1404:1423	the early life stage of piglets	1404:1434	In summary, these results provide important insights and understandings to reveal the relationship between the mucosal microbiota colonization and intestinal function at the early life stage of piglets.
30562014	7	58	theme	microbiota	1353:1362	arg1	colonization					1364:1375	the mucosal microbiota colonization	1341:1375	the mucosal microbiota colonization	1341:1375	In summary, these results provide important insights and understandings to reveal the relationship between the mucosal microbiota colonization and intestinal function at the early life stage of piglets.
30562014	1	59	theme	life	349:352	arg1	GOS					379:381	GOS	379:381	GOS	379:381	Suckling piglets were used to investigate the response of colonic mucosa-associated microbiota composition, mucosal immune homeostasis, and barrier function to early life galactooligosaccharides (GOS) intervention.
30562014	1	59	theme	life	349:352	arg1	galactooligosaccharides					354:376	life galactooligosaccharides	349:376	early life galactooligosaccharides (GOS) intervention	343:395	Suckling piglets were used to investigate the response of colonic mucosa-associated microbiota composition, mucosal immune homeostasis, and barrier function to early life galactooligosaccharides (GOS) intervention.
30562014	0	60	theme	Suckling	165:172	arg1	Piglets					174:180	Suckling Piglets	165:180	Suckling Piglets	165:180	Response of Colonic Mucosa-Associated Microbiota Composition, Mucosal Immune Homeostasis, and Barrier Function to Early Life Galactooligosaccharides Intervention in Suckling Piglets.
30562014	4	61	theme	Unclassified	786:797	arg1	Porphyromonadaceae					799:816	Unclassified Porphyromonadaceae	786:816	Unclassified Porphyromonadaceae	786:816	GOS piglets had a higher abundance of short-chain fatty acids (SCFAs) producer such as Prevotella, Barnesiella, Parabacteroides, and Unclassified Porphyromonadaceae in colonic mucosa ( P < 0.05).
30562014	5	62	theme	SCFAs	872:876	arg1	level					878:882	the total SCFAs level	862:882	the total SCFAs level in colonic digesta of GOS piglets	862:916	In addition, the total SCFAs level in colonic digesta of GOS piglets increased on day 8 ( P < 0.05) and day 21 ( P = 0.064).
30562014	6	63	dep	cytokines	1086:1094	arg1	cytokines					1086:1094	inflammatory cytokines	1073:1094	inflammatory cytokines (IL-8 and IL-10)	1073:1111	Meanwhile, a higher SCFAs concentration in colon of the GOS piglets altered the gene expression of inflammatory cytokines (IL-8 and IL-10) and barrier proteins (ZO-1 and Claudin-1) through regulating the phosphorylation of the NFκB and AMPK signaling pathway.
30562014	6	63	dep	cytokines	1086:1094	arg1	IL-10					1106:1110	IL-10	1106:1110	IL-10	1106:1110	Meanwhile, a higher SCFAs concentration in colon of the GOS piglets altered the gene expression of inflammatory cytokines (IL-8 and IL-10) and barrier proteins (ZO-1 and Claudin-1) through regulating the phosphorylation of the NFκB and AMPK signaling pathway.
30562014	6	63	dep	cytokines	1086:1094	arg1	IL-8					1097:1100	IL-8	1097:1100	IL-8	1097:1100	Meanwhile, a higher SCFAs concentration in colon of the GOS piglets altered the gene expression of inflammatory cytokines (IL-8 and IL-10) and barrier proteins (ZO-1 and Claudin-1) through regulating the phosphorylation of the NFκB and AMPK signaling pathway.
30562014	1	64	theme	galactooligosaccharides	354:376	arg1	intervention					384:395	early life galactooligosaccharides (GOS) intervention	343:395	early life galactooligosaccharides (GOS) intervention	343:395	Suckling piglets were used to investigate the response of colonic mucosa-associated microbiota composition, mucosal immune homeostasis, and barrier function to early life galactooligosaccharides (GOS) intervention.
30562014	4	65	theme	fatty	703:707	arg1	SCFAs					716:720	SCFAs	716:720	SCFAs	716:720	GOS piglets had a higher abundance of short-chain fatty acids (SCFAs) producer such as Prevotella, Barnesiella, Parabacteroides, and Unclassified Porphyromonadaceae in colonic mucosa ( P < 0.05).
30562014	4	65	theme	fatty	703:707	arg1	acids					709:713	short-chain fatty acids	691:713	short-chain fatty acids (SCFAs) producer such as Prevotella, Barnesiella, Parabacteroides, and Unclassified Porphyromonadaceae in colonic mucosa ( P < 0.05)	691:846	GOS piglets had a higher abundance of short-chain fatty acids (SCFAs) producer such as Prevotella, Barnesiella, Parabacteroides, and Unclassified Porphyromonadaceae in colonic mucosa ( P < 0.05).
30562014	4	65	theme	fatty	703:707	arg1	Parabacteroides					765:779	Parabacteroides	765:779	Parabacteroides	765:779	GOS piglets had a higher abundance of short-chain fatty acids (SCFAs) producer such as Prevotella, Barnesiella, Parabacteroides, and Unclassified Porphyromonadaceae in colonic mucosa ( P < 0.05).
30562014	4	65	theme	fatty	703:707	arg1	Barnesiella					752:762	Barnesiella	752:762	Barnesiella	752:762	GOS piglets had a higher abundance of short-chain fatty acids (SCFAs) producer such as Prevotella, Barnesiella, Parabacteroides, and Unclassified Porphyromonadaceae in colonic mucosa ( P < 0.05).
30562014	4	65	theme	fatty	703:707	arg1	Porphyromonadaceae					799:816	Unclassified Porphyromonadaceae	786:816	Unclassified Porphyromonadaceae	786:816	GOS piglets had a higher abundance of short-chain fatty acids (SCFAs) producer such as Prevotella, Barnesiella, Parabacteroides, and Unclassified Porphyromonadaceae in colonic mucosa ( P < 0.05).
30562014	6	66	theme	SCFAs	994:998	arg1	concentration					1000:1012	a higher SCFAs concentration	985:1012	a higher SCFAs concentration in colon of the GOS piglets	985:1040	Meanwhile, a higher SCFAs concentration in colon of the GOS piglets altered the gene expression of inflammatory cytokines (IL-8 and IL-10) and barrier proteins (ZO-1 and Claudin-1) through regulating the phosphorylation of the NFκB and AMPK signaling pathway.
30562014	6	66	theme	SCFAs	994:998	arg1	Meanwhile					974:982	Meanwhile	974:982	Meanwhile	974:982	Meanwhile, a higher SCFAs concentration in colon of the GOS piglets altered the gene expression of inflammatory cytokines (IL-8 and IL-10) and barrier proteins (ZO-1 and Claudin-1) through regulating the phosphorylation of the NFκB and AMPK signaling pathway.
30562014	1	67	theme	colonic	241:247	arg1	composition					278:288	colonic mucosa-associated microbiota composition	241:288	colonic mucosa-associated microbiota composition	241:288	Suckling piglets were used to investigate the response of colonic mucosa-associated microbiota composition, mucosal immune homeostasis, and barrier function to early life galactooligosaccharides (GOS) intervention.
30562014	2	68	theme	week	563:566	arg1	time					568:571	a week time	561:571	a week time	561:571	Ten milliliter 1 g/kg body weight GOS solutions and physiological saline solutions were fed to the newborn piglets in the GOS group and in the control (CON) group a week time, respectively.
30562014	7	69	theme	intestinal	1381:1390	arg1	function					1392:1399	intestinal function	1381:1399	intestinal function	1381:1399	In summary, these results provide important insights and understandings to reveal the relationship between the mucosal microbiota colonization and intestinal function at the early life stage of piglets.
30562014	1	70	used	used	205:208	arg2	piglets					192:198	Suckling piglets	183:198	Suckling piglets	183:198	Suckling piglets were used to investigate the response of colonic mucosa-associated microbiota composition, mucosal immune homeostasis, and barrier function to early life galactooligosaccharides (GOS) intervention.
30562014	4	71	theme	short-chain	691:701	arg1	SCFAs					716:720	SCFAs	716:720	SCFAs	716:720	GOS piglets had a higher abundance of short-chain fatty acids (SCFAs) producer such as Prevotella, Barnesiella, Parabacteroides, and Unclassified Porphyromonadaceae in colonic mucosa ( P < 0.05).
30562014	4	71	theme	short-chain	691:701	arg1	acids					709:713	short-chain fatty acids	691:713	short-chain fatty acids (SCFAs) producer such as Prevotella, Barnesiella, Parabacteroides, and Unclassified Porphyromonadaceae in colonic mucosa ( P < 0.05)	691:846	GOS piglets had a higher abundance of short-chain fatty acids (SCFAs) producer such as Prevotella, Barnesiella, Parabacteroides, and Unclassified Porphyromonadaceae in colonic mucosa ( P < 0.05).
30562014	4	71	theme	short-chain	691:701	arg1	Parabacteroides					765:779	Parabacteroides	765:779	Parabacteroides	765:779	GOS piglets had a higher abundance of short-chain fatty acids (SCFAs) producer such as Prevotella, Barnesiella, Parabacteroides, and Unclassified Porphyromonadaceae in colonic mucosa ( P < 0.05).
30562014	4	71	theme	short-chain	691:701	arg1	Barnesiella					752:762	Barnesiella	752:762	Barnesiella	752:762	GOS piglets had a higher abundance of short-chain fatty acids (SCFAs) producer such as Prevotella, Barnesiella, Parabacteroides, and Unclassified Porphyromonadaceae in colonic mucosa ( P < 0.05).
30562014	4	71	theme	short-chain	691:701	arg1	Porphyromonadaceae					799:816	Unclassified Porphyromonadaceae	786:816	Unclassified Porphyromonadaceae	786:816	GOS piglets had a higher abundance of short-chain fatty acids (SCFAs) producer such as Prevotella, Barnesiella, Parabacteroides, and Unclassified Porphyromonadaceae in colonic mucosa ( P < 0.05).
30562014	6	72	theme	pathway	1225:1231	arg1	phosphorylation					1178:1192	the phosphorylation	1174:1192	the phosphorylation of the NFκB and AMPK signaling pathway	1174:1231	Meanwhile, a higher SCFAs concentration in colon of the GOS piglets altered the gene expression of inflammatory cytokines (IL-8 and IL-10) and barrier proteins (ZO-1 and Claudin-1) through regulating the phosphorylation of the NFκB and AMPK signaling pathway.
30562014	1	73	theme	mucosa-associated	249:265	arg1	composition					278:288	colonic mucosa-associated microbiota composition	241:288	colonic mucosa-associated microbiota composition	241:288	Suckling piglets were used to investigate the response of colonic mucosa-associated microbiota composition, mucosal immune homeostasis, and barrier function to early life galactooligosaccharides (GOS) intervention.
30562014	0	74	theme	Homeostasis	77:87	arg1	Response					0:7	Response	0:7	Response of Colonic Mucosa-Associated Microbiota Composition, Mucosal Immune Homeostasis, and Barrier Function to Early Life	0:123	Response of Colonic Mucosa-Associated Microbiota Composition, Mucosal Immune Homeostasis, and Barrier Function to Early Life Galactooligosaccharides Intervention in Suckling Piglets.
30562014	6	75	theme	inflammatory	1073:1084	arg1	cytokines					1086:1094	inflammatory cytokines	1073:1094	inflammatory cytokines (IL-8 and IL-10)	1073:1111	Meanwhile, a higher SCFAs concentration in colon of the GOS piglets altered the gene expression of inflammatory cytokines (IL-8 and IL-10) and barrier proteins (ZO-1 and Claudin-1) through regulating the phosphorylation of the NFκB and AMPK signaling pathway.
30562014	6	75	theme	inflammatory	1073:1084	arg1	IL-10					1106:1110	IL-10	1106:1110	IL-10	1106:1110	Meanwhile, a higher SCFAs concentration in colon of the GOS piglets altered the gene expression of inflammatory cytokines (IL-8 and IL-10) and barrier proteins (ZO-1 and Claudin-1) through regulating the phosphorylation of the NFκB and AMPK signaling pathway.
30562014	6	75	theme	inflammatory	1073:1084	arg1	IL-8					1097:1100	IL-8	1097:1100	IL-8	1097:1100	Meanwhile, a higher SCFAs concentration in colon of the GOS piglets altered the gene expression of inflammatory cytokines (IL-8 and IL-10) and barrier proteins (ZO-1 and Claudin-1) through regulating the phosphorylation of the NFκB and AMPK signaling pathway.
30562014	6	76	theme	higher	987:992	arg1	concentration					1000:1012	a higher SCFAs concentration	985:1012	a higher SCFAs concentration in colon of the GOS piglets	985:1040	Meanwhile, a higher SCFAs concentration in colon of the GOS piglets altered the gene expression of inflammatory cytokines (IL-8 and IL-10) and barrier proteins (ZO-1 and Claudin-1) through regulating the phosphorylation of the NFκB and AMPK signaling pathway.
30562014	6	76	theme	higher	987:992	arg1	Meanwhile					974:982	Meanwhile	974:982	Meanwhile	974:982	Meanwhile, a higher SCFAs concentration in colon of the GOS piglets altered the gene expression of inflammatory cytokines (IL-8 and IL-10) and barrier proteins (ZO-1 and Claudin-1) through regulating the phosphorylation of the NFκB and AMPK signaling pathway.
30562014	1	77	theme	microbiota	267:276	arg1	composition					278:288	colonic mucosa-associated microbiota composition	241:288	colonic mucosa-associated microbiota composition	241:288	Suckling piglets were used to investigate the response of colonic mucosa-associated microbiota composition, mucosal immune homeostasis, and barrier function to early life galactooligosaccharides (GOS) intervention.
30562014	0	78	theme	Immune	70:75	arg1	Homeostasis					77:87	Mucosal Immune Homeostasis	62:87	Mucosal Immune Homeostasis	62:87	Response of Colonic Mucosa-Associated Microbiota Composition, Mucosal Immune Homeostasis, and Barrier Function to Early Life Galactooligosaccharides Intervention in Suckling Piglets.
30562014	2	79	from	group	555:559	arg1	group					524:528	the GOS group	516:528	the GOS group	516:528	Ten milliliter 1 g/kg body weight GOS solutions and physiological saline solutions were fed to the newborn piglets in the GOS group and in the control (CON) group a week time, respectively.
30562014	2	80	theme	milliliter	402:411	arg1	weight					425:430	Ten milliliter 1 g/kg body weight	398:430	Ten milliliter 1 g/kg body weight GOS solutions	398:444	Ten milliliter 1 g/kg body weight GOS solutions and physiological saline solutions were fed to the newborn piglets in the GOS group and in the control (CON) group a week time, respectively.
30562014	1	81	theme	composition	278:288	arg1	response					229:236	the response	225:236	the response of colonic mucosa-associated microbiota composition, mucosal immune homeostasis, and barrier function to early life galactooligosaccharides (GOS) intervention	225:395	Suckling piglets were used to investigate the response of colonic mucosa-associated microbiota composition, mucosal immune homeostasis, and barrier function to early life galactooligosaccharides (GOS) intervention.
31701250	6	0	from	3.3 μM	1262:1267	arg1	samples					1356:1362	spiked serum samples	1343:1362	spiked serum samples	1343:1362	The following figures of merit were found for simultaneous electrochemical determination of 5-HT, DA, and AA: (a) Well separated peaks at around 0.316, 0.16 and - 0.044 V; (b) linear responses in the 0.01 - 25 μM, 0.02 - 25 μM and 10 - 350 μM; (c) detection limits of 3.3 nM, 6.7 nM and 3.3 μM (S/N = 3), and (d) recoveries of 96.9-103%, 97.3%-102% and 96.3-105% from spiked serum samples, respectively.
31701250	2	1	theme	carbon	389:394	arg1	GCE					407:409	GCE	407:409	GCE	407:409	The composite was placed on a glassy carbon electrode (GCE) to design an electrochemical sensor for detecting simultaneously serotonin (5-HT), dopamine (DA), and ascorbic acid (AA).
31701250	2	1	theme	carbon	389:394	arg1	electrode					396:404	a glassy carbon electrode	380:404	a glassy carbon electrode (GCE)	380:410	The composite was placed on a glassy carbon electrode (GCE) to design an electrochemical sensor for detecting simultaneously serotonin (5-HT), dopamine (DA), and ascorbic acid (AA).
31701250	6	2	theme	merit	1000:1004	arg1	figures					989:995	The following figures	975:995	The following figures of merit	975:1004	The following figures of merit were found for simultaneous electrochemical determination of 5-HT, DA, and AA: (a) Well separated peaks at around 0.316, 0.16 and - 0.044 V; (b) linear responses in the 0.01 - 25 μM, 0.02 - 25 μM and 10 - 350 μM; (c) detection limits of 3.3 nM, 6.7 nM and 3.3 μM (S/N = 3), and (d) recoveries of 96.9-103%, 97.3%-102% and 96.3-105% from spiked serum samples, respectively.
31701250	6	3	theme	5-HT	1067:1070	arg1	determination					1050:1062	simultaneous electrochemical determination	1021:1062	simultaneous electrochemical determination of 5-HT, DA, and AA	1021:1082	The following figures of merit were found for simultaneous electrochemical determination of 5-HT, DA, and AA: (a) Well separated peaks at around 0.316, 0.16 and - 0.044 V; (b) linear responses in the 0.01 - 25 μM, 0.02 - 25 μM and 10 - 350 μM; (c) detection limits of 3.3 nM, 6.7 nM and 3.3 μM (S/N = 3), and (d) recoveries of 96.9-103%, 97.3%-102% and 96.3-105% from spiked serum samples, respectively.
31701250	2	4	theme	glassy	382:387	arg1	GCE					407:409	GCE	407:409	GCE	407:409	The composite was placed on a glassy carbon electrode (GCE) to design an electrochemical sensor for detecting simultaneously serotonin (5-HT), dopamine (DA), and ascorbic acid (AA).
31701250	2	4	theme	glassy	382:387	arg1	electrode					396:404	a glassy carbon electrode	380:404	a glassy carbon electrode (GCE)	380:410	The composite was placed on a glassy carbon electrode (GCE) to design an electrochemical sensor for detecting simultaneously serotonin (5-HT), dopamine (DA), and ascorbic acid (AA).
31701250	6	5	from	responses	1158:1166	arg1	0.01					1175:1178	0.01	1175:1178	0.01	1175:1178	The following figures of merit were found for simultaneous electrochemical determination of 5-HT, DA, and AA: (a) Well separated peaks at around 0.316, 0.16 and - 0.044 V; (b) linear responses in the 0.01 - 25 μM, 0.02 - 25 μM and 10 - 350 μM; (c) detection limits of 3.3 nM, 6.7 nM and 3.3 μM (S/N = 3), and (d) recoveries of 96.9-103%, 97.3%-102% and 96.3-105% from spiked serum samples, respectively.
31701250	6	5	from	responses	1158:1166	arg1	10					1206:1207	10	1206:1207	10	1206:1207	The following figures of merit were found for simultaneous electrochemical determination of 5-HT, DA, and AA: (a) Well separated peaks at around 0.316, 0.16 and - 0.044 V; (b) linear responses in the 0.01 - 25 μM, 0.02 - 25 μM and 10 - 350 μM; (c) detection limits of 3.3 nM, 6.7 nM and 3.3 μM (S/N = 3), and (d) recoveries of 96.9-103%, 97.3%-102% and 96.3-105% from spiked serum samples, respectively.
31701250	6	5	from	responses	1158:1166	arg1	25 μM					1196:1200	25 μM	1196:1200	25 μM	1196:1200	The following figures of merit were found for simultaneous electrochemical determination of 5-HT, DA, and AA: (a) Well separated peaks at around 0.316, 0.16 and - 0.044 V; (b) linear responses in the 0.01 - 25 μM, 0.02 - 25 μM and 10 - 350 μM; (c) detection limits of 3.3 nM, 6.7 nM and 3.3 μM (S/N = 3), and (d) recoveries of 96.9-103%, 97.3%-102% and 96.3-105% from spiked serum samples, respectively.
31701250	3	6	theme	electrical	660:669	arg1	conductivity					671:682	good electrical conductivity	655:682	good electrical conductivity	655:682	The interconnected porous reduced graphene oxide framework tightly anchored to the Fe3O4 magnetic nanoparticles warrants good electrical conductivity and efficient catalytic activity.
31701250	6	7	theme	linear	1151:1156	arg1	responses					1158:1166	(b) linear responses	1147:1166	(b) linear responses in the 0.01 - 25 μM, 0.02 - 25 μM and 10 - 350 μM	1147:1216	The following figures of merit were found for simultaneous electrochemical determination of 5-HT, DA, and AA: (a) Well separated peaks at around 0.316, 0.16 and - 0.044 V; (b) linear responses in the 0.01 - 25 μM, 0.02 - 25 μM and 10 - 350 μM; (c) detection limits of 3.3 nM, 6.7 nM and 3.3 μM (S/N = 3), and (d) recoveries of 96.9-103%, 97.3%-102% and 96.3-105% from spiked serum samples, respectively.
31701250	4	8	theme	recognition	770:780	arg1	ability					782:788	high recognition ability	765:788	high recognition ability	765:788	The HP-β-CD acts as a supramolecular host with high recognition ability for 5-HT, DA and AA.
31701250	4	9	theme	high	765:768	arg1	ability					782:788	high recognition ability	765:788	high recognition ability	765:788	The HP-β-CD acts as a supramolecular host with high recognition ability for 5-HT, DA and AA.
31701250	6	10	dep	found	1011:1015	arg1	responses					1158:1166	(b) linear responses	1147:1166	(b) linear responses in the 0.01 - 25 μM, 0.02 - 25 μM and 10 - 350 μM	1147:1216	The following figures of merit were found for simultaneous electrochemical determination of 5-HT, DA, and AA: (a) Well separated peaks at around 0.316, 0.16 and - 0.044 V; (b) linear responses in the 0.01 - 25 μM, 0.02 - 25 μM and 10 - 350 μM; (c) detection limits of 3.3 nM, 6.7 nM and 3.3 μM (S/N = 3), and (d) recoveries of 96.9-103%, 97.3%-102% and 96.3-105% from spiked serum samples, respectively.
31701250	6	10	dep	found	1011:1015	arg1	limits					1233:1238	(c) detection limits	1219:1238	(c) detection limits of 3.3 nM, 6.7 nM and 3.3 μM (S/N = 3), and (d) recoveries of 96.9-103%, 97.3%-102% and 96.3-105% from spiked serum samples, respectively	1219:1376	The following figures of merit were found for simultaneous electrochemical determination of 5-HT, DA, and AA: (a) Well separated peaks at around 0.316, 0.16 and - 0.044 V; (b) linear responses in the 0.01 - 25 μM, 0.02 - 25 μM and 10 - 350 μM; (c) detection limits of 3.3 nM, 6.7 nM and 3.3 μM (S/N = 3), and (d) recoveries of 96.9-103%, 97.3%-102% and 96.3-105% from spiked serum samples, respectively.
31701250	6	10	dep	found	1011:1015	arg1	peaks					1104:1108	(a) Well separated peaks	1085:1108	(a) Well separated peaks at around 0.316, 0.16 and - 0.044 V	1085:1144	The following figures of merit were found for simultaneous electrochemical determination of 5-HT, DA, and AA: (a) Well separated peaks at around 0.316, 0.16 and - 0.044 V; (b) linear responses in the 0.01 - 25 μM, 0.02 - 25 μM and 10 - 350 μM; (c) detection limits of 3.3 nM, 6.7 nM and 3.3 μM (S/N = 3), and (d) recoveries of 96.9-103%, 97.3%-102% and 96.3-105% from spiked serum samples, respectively.
31701250	8	11	theme	pulse	1678:1682	arg1	DPV					1697:1699	DPV	1697:1699	DPV	1697:1699	Graphical abstractSchematic representation of simultaneous detecting serotonin (5-HT), dopamine (DA) and ascorbic acid (AA) for three-dimensional reduced-graphene oxide/Fe3O4/hydroxypropyl-β-cyclodextrin (3D-rGO/Fe3O4/HP-β-CD) by differential pulse voltammetry (DPV) approach.
31701250	8	11	theme	pulse	1678:1682	arg1	voltammetry					1684:1694	differential pulse voltammetry	1665:1694	differential pulse voltammetry (DPV) approach	1665:1709	Graphical abstractSchematic representation of simultaneous detecting serotonin (5-HT), dopamine (DA) and ascorbic acid (AA) for three-dimensional reduced-graphene oxide/Fe3O4/hydroxypropyl-β-cyclodextrin (3D-rGO/Fe3O4/HP-β-CD) by differential pulse voltammetry (DPV) approach.
31701250	6	12	from	samples	1356:1362	arg1	%					1310:1310	96.9-103%	1302:1310	96.9-103%	1302:1310	The following figures of merit were found for simultaneous electrochemical determination of 5-HT, DA, and AA: (a) Well separated peaks at around 0.316, 0.16 and - 0.044 V; (b) linear responses in the 0.01 - 25 μM, 0.02 - 25 μM and 10 - 350 μM; (c) detection limits of 3.3 nM, 6.7 nM and 3.3 μM (S/N = 3), and (d) recoveries of 96.9-103%, 97.3%-102% and 96.3-105% from spiked serum samples, respectively.
31701250	6	12	from	samples	1356:1362	arg1	3.3 μM					1262:1267	3.3 μM	1262:1267	3.3 μM (S/N = 3)	1262:1277	The following figures of merit were found for simultaneous electrochemical determination of 5-HT, DA, and AA: (a) Well separated peaks at around 0.316, 0.16 and - 0.044 V; (b) linear responses in the 0.01 - 25 μM, 0.02 - 25 μM and 10 - 350 μM; (c) detection limits of 3.3 nM, 6.7 nM and 3.3 μM (S/N = 3), and (d) recoveries of 96.9-103%, 97.3%-102% and 96.3-105% from spiked serum samples, respectively.
31701250	6	12	from	samples	1356:1362	arg1	%					1336:1336	96.3-105%	1328:1336	96.3-105% from spiked serum samples	1328:1362	The following figures of merit were found for simultaneous electrochemical determination of 5-HT, DA, and AA: (a) Well separated peaks at around 0.316, 0.16 and - 0.044 V; (b) linear responses in the 0.01 - 25 μM, 0.02 - 25 μM and 10 - 350 μM; (c) detection limits of 3.3 nM, 6.7 nM and 3.3 μM (S/N = 3), and (d) recoveries of 96.9-103%, 97.3%-102% and 96.3-105% from spiked serum samples, respectively.
31701250	6	12	from	samples	1356:1362	arg1	S/N = 3					1270:1276	S/N = 3	1270:1276	S/N = 3	1270:1276	The following figures of merit were found for simultaneous electrochemical determination of 5-HT, DA, and AA: (a) Well separated peaks at around 0.316, 0.16 and - 0.044 V; (b) linear responses in the 0.01 - 25 μM, 0.02 - 25 μM and 10 - 350 μM; (c) detection limits of 3.3 nM, 6.7 nM and 3.3 μM (S/N = 3), and (d) recoveries of 96.9-103%, 97.3%-102% and 96.3-105% from spiked serum samples, respectively.
31701250	6	12	from	samples	1356:1362	arg1	recoveries					1288:1297	(d) recoveries	1284:1297	(d) recoveries	1284:1297	The following figures of merit were found for simultaneous electrochemical determination of 5-HT, DA, and AA: (a) Well separated peaks at around 0.316, 0.16 and - 0.044 V; (b) linear responses in the 0.01 - 25 μM, 0.02 - 25 μM and 10 - 350 μM; (c) detection limits of 3.3 nM, 6.7 nM and 3.3 μM (S/N = 3), and (d) recoveries of 96.9-103%, 97.3%-102% and 96.3-105% from spiked serum samples, respectively.
31701250	6	12	from	samples	1356:1362	arg1	3.3 nM					1243:1248	3.3 nM	1243:1248	3.3 nM	1243:1248	The following figures of merit were found for simultaneous electrochemical determination of 5-HT, DA, and AA: (a) Well separated peaks at around 0.316, 0.16 and - 0.044 V; (b) linear responses in the 0.01 - 25 μM, 0.02 - 25 μM and 10 - 350 μM; (c) detection limits of 3.3 nM, 6.7 nM and 3.3 μM (S/N = 3), and (d) recoveries of 96.9-103%, 97.3%-102% and 96.3-105% from spiked serum samples, respectively.
31701250	6	12	from	samples	1356:1362	arg1	6.7 nM					1251:1256	6.7 nM	1251:1256	6.7 nM	1251:1256	The following figures of merit were found for simultaneous electrochemical determination of 5-HT, DA, and AA: (a) Well separated peaks at around 0.316, 0.16 and - 0.044 V; (b) linear responses in the 0.01 - 25 μM, 0.02 - 25 μM and 10 - 350 μM; (c) detection limits of 3.3 nM, 6.7 nM and 3.3 μM (S/N = 3), and (d) recoveries of 96.9-103%, 97.3%-102% and 96.3-105% from spiked serum samples, respectively.
31701250	6	12	from	samples	1356:1362	arg1	%					1322:1322	97.3%-102%	1313:1322	97.3%-102%	1313:1322	The following figures of merit were found for simultaneous electrochemical determination of 5-HT, DA, and AA: (a) Well separated peaks at around 0.316, 0.16 and - 0.044 V; (b) linear responses in the 0.01 - 25 μM, 0.02 - 25 μM and 10 - 350 μM; (c) detection limits of 3.3 nM, 6.7 nM and 3.3 μM (S/N = 3), and (d) recoveries of 96.9-103%, 97.3%-102% and 96.3-105% from spiked serum samples, respectively.
31701250	6	13	from	and - 0.044 V	1132:1144	arg1	peaks					1104:1108	(a) Well separated peaks	1085:1108	(a) Well separated peaks at around 0.316, 0.16 and - 0.044 V	1085:1144	The following figures of merit were found for simultaneous electrochemical determination of 5-HT, DA, and AA: (a) Well separated peaks at around 0.316, 0.16 and - 0.044 V; (b) linear responses in the 0.01 - 25 μM, 0.02 - 25 μM and 10 - 350 μM; (c) detection limits of 3.3 nM, 6.7 nM and 3.3 μM (S/N = 3), and (d) recoveries of 96.9-103%, 97.3%-102% and 96.3-105% from spiked serum samples, respectively.
31701250	6	14	theme	following	979:987	arg1	figures					989:995	The following figures	975:995	The following figures of merit	975:1004	The following figures of merit were found for simultaneous electrochemical determination of 5-HT, DA, and AA: (a) Well separated peaks at around 0.316, 0.16 and - 0.044 V; (b) linear responses in the 0.01 - 25 μM, 0.02 - 25 μM and 10 - 350 μM; (c) detection limits of 3.3 nM, 6.7 nM and 3.3 μM (S/N = 3), and (d) recoveries of 96.9-103%, 97.3%-102% and 96.3-105% from spiked serum samples, respectively.
31701250	2	15	theme	ascorbic	514:521	arg1	AA					529:530	AA	529:530	AA	529:530	The composite was placed on a glassy carbon electrode (GCE) to design an electrochemical sensor for detecting simultaneously serotonin (5-HT), dopamine (DA), and ascorbic acid (AA).
31701250	2	15	theme	ascorbic	514:521	arg1	acid					523:526	ascorbic acid	514:526	ascorbic acid (AA)	514:531	The composite was placed on a glassy carbon electrode (GCE) to design an electrochemical sensor for detecting simultaneously serotonin (5-HT), dopamine (DA), and ascorbic acid (AA).
31701250	6	16	from	3.3 nM	1243:1248	arg1	samples					1356:1362	spiked serum samples	1343:1362	spiked serum samples	1343:1362	The following figures of merit were found for simultaneous electrochemical determination of 5-HT, DA, and AA: (a) Well separated peaks at around 0.316, 0.16 and - 0.044 V; (b) linear responses in the 0.01 - 25 μM, 0.02 - 25 μM and 10 - 350 μM; (c) detection limits of 3.3 nM, 6.7 nM and 3.3 μM (S/N = 3), and (d) recoveries of 96.9-103%, 97.3%-102% and 96.3-105% from spiked serum samples, respectively.
31701250	8	17	theme	differential	1665:1676	arg1	DPV					1697:1699	DPV	1697:1699	DPV	1697:1699	Graphical abstractSchematic representation of simultaneous detecting serotonin (5-HT), dopamine (DA) and ascorbic acid (AA) for three-dimensional reduced-graphene oxide/Fe3O4/hydroxypropyl-β-cyclodextrin (3D-rGO/Fe3O4/HP-β-CD) by differential pulse voltammetry (DPV) approach.
31701250	8	17	theme	differential	1665:1676	arg1	voltammetry					1684:1694	differential pulse voltammetry	1665:1694	differential pulse voltammetry (DPV) approach	1665:1709	Graphical abstractSchematic representation of simultaneous detecting serotonin (5-HT), dopamine (DA) and ascorbic acid (AA) for three-dimensional reduced-graphene oxide/Fe3O4/hydroxypropyl-β-cyclodextrin (3D-rGO/Fe3O4/HP-β-CD) by differential pulse voltammetry (DPV) approach.
31701250	8	18	theme	serotonin	1504:1512	arg1	representation					1463:1476	Graphical abstractSchematic representation	1435:1476	Graphical abstractSchematic representation of simultaneous detecting serotonin (5-HT), dopamine (DA) and ascorbic acid (AA) for three-dimensional reduced-graphene oxide/Fe3O4/hydroxypropyl-β-cyclodextrin (3D-rGO/Fe3O4/HP-β-CD) by differential pulse voltammetry (DPV) approach.	1435:1710	Graphical abstractSchematic representation of simultaneous detecting serotonin (5-HT), dopamine (DA) and ascorbic acid (AA) for three-dimensional reduced-graphene oxide/Fe3O4/hydroxypropyl-β-cyclodextrin (3D-rGO/Fe3O4/HP-β-CD) by differential pulse voltammetry (DPV) approach.
31701250	1	19	theme	Reduced	181:187	arg1	oxide					198:202	Reduced graphene oxide	181:202	Reduced graphene oxide containing Fe3O4 nanoparticles	181:233	Reduced graphene oxide containing Fe3O4 nanoparticles was decorated with hydroxypropyl-β-cyclodextrin (HP-β-CD) to construct a novel nanocomposite (3D-rGO/Fe3O4/HP-β-CD).
31701250	5	20	theme	Well-separated	811:824	arg1	peaks					836:840	Well-separated oxidation peaks	811:840	Well-separated oxidation peaks	811:840	Well-separated oxidation peaks and increased peak currents were observed for 5-HT, DA, and AA individually and in mixtures by differential pulse voltammetry (DPV).
31701250	7	21	theme	standard	1392:1399	arg1	RSD					1412:1414	RSD	1412:1414	RSD	1412:1414	All relative standard deviation (RSD) are less than 4%.
31701250	7	21	theme	standard	1392:1399	arg1	%					1432:1432	less than 4%	1421:1432	less than 4%	1421:1432	All relative standard deviation (RSD) are less than 4%.
31701250	7	21	theme	standard	1392:1399	arg1	deviation					1401:1409	All relative standard deviation	1379:1409	All relative standard deviation (RSD)	1379:1415	All relative standard deviation (RSD) are less than 4%.
31701250	5	22	theme	differential	937:948	arg1	voltammetry					956:966	differential pulse voltammetry	937:966	differential pulse voltammetry (DPV)	937:972	Well-separated oxidation peaks and increased peak currents were observed for 5-HT, DA, and AA individually and in mixtures by differential pulse voltammetry (DPV).
31701250	5	22	theme	differential	937:948	arg1	DPV					969:971	DPV	969:971	DPV	969:971	Well-separated oxidation peaks and increased peak currents were observed for 5-HT, DA, and AA individually and in mixtures by differential pulse voltammetry (DPV).
31701250	6	23	theme	AA	1081:1082	arg1	determination					1050:1062	simultaneous electrochemical determination	1021:1062	simultaneous electrochemical determination of 5-HT, DA, and AA	1021:1082	The following figures of merit were found for simultaneous electrochemical determination of 5-HT, DA, and AA: (a) Well separated peaks at around 0.316, 0.16 and - 0.044 V; (b) linear responses in the 0.01 - 25 μM, 0.02 - 25 μM and 10 - 350 μM; (c) detection limits of 3.3 nM, 6.7 nM and 3.3 μM (S/N = 3), and (d) recoveries of 96.9-103%, 97.3%-102% and 96.3-105% from spiked serum samples, respectively.
31701250	1	24	theme	graphene	189:196	arg1	oxide					198:202	Reduced graphene oxide	181:202	Reduced graphene oxide containing Fe3O4 nanoparticles	181:233	Reduced graphene oxide containing Fe3O4 nanoparticles was decorated with hydroxypropyl-β-cyclodextrin (HP-β-CD) to construct a novel nanocomposite (3D-rGO/Fe3O4/HP-β-CD).
31701250	0	25	theme	electrochemical	13:27	arg1	sensing					29:35	Simultaneous electrochemical sensing	0:35	Simultaneous electrochemical sensing of serotonin, dopamine and ascorbic acid by using a nanocomposite prepared from reduced graphene oxide, Fe3O4 and hydroxypropyl-β-cyclodextrin.	0:179	Simultaneous electrochemical sensing of serotonin, dopamine and ascorbic acid by using a nanocomposite prepared from reduced graphene oxide, Fe3O4 and hydroxypropyl-β-cyclodextrin.
31701250	6	26	from	6.7 nM	1251:1256	arg1	samples					1356:1362	spiked serum samples	1343:1362	spiked serum samples	1343:1362	The following figures of merit were found for simultaneous electrochemical determination of 5-HT, DA, and AA: (a) Well separated peaks at around 0.316, 0.16 and - 0.044 V; (b) linear responses in the 0.01 - 25 μM, 0.02 - 25 μM and 10 - 350 μM; (c) detection limits of 3.3 nM, 6.7 nM and 3.3 μM (S/N = 3), and (d) recoveries of 96.9-103%, 97.3%-102% and 96.3-105% from spiked serum samples, respectively.
31701250	8	27	theme	voltammetry	1684:1694	arg1	approach					1702:1709	differential pulse voltammetry (DPV) approach	1665:1709	differential pulse voltammetry (DPV) approach	1665:1709	Graphical abstractSchematic representation of simultaneous detecting serotonin (5-HT), dopamine (DA) and ascorbic acid (AA) for three-dimensional reduced-graphene oxide/Fe3O4/hydroxypropyl-β-cyclodextrin (3D-rGO/Fe3O4/HP-β-CD) by differential pulse voltammetry (DPV) approach.
31701250	3	28	theme	reduced	560:566	arg1	framework					583:591	The interconnected porous reduced graphene oxide framework	534:591	The interconnected porous reduced graphene oxide framework tightly anchored to the Fe3O4 magnetic nanoparticles	534:644	The interconnected porous reduced graphene oxide framework tightly anchored to the Fe3O4 magnetic nanoparticles warrants good electrical conductivity and efficient catalytic activity.
31701250	6	29	theme	DA	1073:1074	arg1	determination					1050:1062	simultaneous electrochemical determination	1021:1062	simultaneous electrochemical determination of 5-HT, DA, and AA	1021:1082	The following figures of merit were found for simultaneous electrochemical determination of 5-HT, DA, and AA: (a) Well separated peaks at around 0.316, 0.16 and - 0.044 V; (b) linear responses in the 0.01 - 25 μM, 0.02 - 25 μM and 10 - 350 μM; (c) detection limits of 3.3 nM, 6.7 nM and 3.3 μM (S/N = 3), and (d) recoveries of 96.9-103%, 97.3%-102% and 96.3-105% from spiked serum samples, respectively.
31701250	0	30	theme	Simultaneous	0:11	arg1	sensing					29:35	Simultaneous electrochemical sensing	0:35	Simultaneous electrochemical sensing of serotonin, dopamine and ascorbic acid by using a nanocomposite prepared from reduced graphene oxide, Fe3O4 and hydroxypropyl-β-cyclodextrin.	0:179	Simultaneous electrochemical sensing of serotonin, dopamine and ascorbic acid by using a nanocomposite prepared from reduced graphene oxide, Fe3O4 and hydroxypropyl-β-cyclodextrin.
31701250	1	31	theme	novel	308:312	arg1	3D-rGO/Fe3O4/HP-β-CD					329:348	3D-rGO/Fe3O4/HP-β-CD	329:348	3D-rGO/Fe3O4/HP-β-CD	329:348	Reduced graphene oxide containing Fe3O4 nanoparticles was decorated with hydroxypropyl-β-cyclodextrin (HP-β-CD) to construct a novel nanocomposite (3D-rGO/Fe3O4/HP-β-CD).
31701250	1	31	theme	novel	308:312	arg1	nanocomposite					314:326	a novel nanocomposite	306:326	a novel nanocomposite (3D-rGO/Fe3O4/HP-β-CD)	306:349	Reduced graphene oxide containing Fe3O4 nanoparticles was decorated with hydroxypropyl-β-cyclodextrin (HP-β-CD) to construct a novel nanocomposite (3D-rGO/Fe3O4/HP-β-CD).
31701250	6	32	from	recoveries	1288:1297	arg1	samples					1356:1362	spiked serum samples	1343:1362	spiked serum samples	1343:1362	The following figures of merit were found for simultaneous electrochemical determination of 5-HT, DA, and AA: (a) Well separated peaks at around 0.316, 0.16 and - 0.044 V; (b) linear responses in the 0.01 - 25 μM, 0.02 - 25 μM and 10 - 350 μM; (c) detection limits of 3.3 nM, 6.7 nM and 3.3 μM (S/N = 3), and (d) recoveries of 96.9-103%, 97.3%-102% and 96.3-105% from spiked serum samples, respectively.
31701250	3	33	theme	graphene	568:575	arg1	framework					583:591	The interconnected porous reduced graphene oxide framework	534:591	The interconnected porous reduced graphene oxide framework tightly anchored to the Fe3O4 magnetic nanoparticles	534:644	The interconnected porous reduced graphene oxide framework tightly anchored to the Fe3O4 magnetic nanoparticles warrants good electrical conductivity and efficient catalytic activity.
31701250	6	34	theme	serum	1350:1354	arg1	samples					1356:1362	spiked serum samples	1343:1362	spiked serum samples	1343:1362	The following figures of merit were found for simultaneous electrochemical determination of 5-HT, DA, and AA: (a) Well separated peaks at around 0.316, 0.16 and - 0.044 V; (b) linear responses in the 0.01 - 25 μM, 0.02 - 25 μM and 10 - 350 μM; (c) detection limits of 3.3 nM, 6.7 nM and 3.3 μM (S/N = 3), and (d) recoveries of 96.9-103%, 97.3%-102% and 96.3-105% from spiked serum samples, respectively.
31701250	8	35	theme	Graphical	1435:1443	arg1	representation					1463:1476	Graphical abstractSchematic representation	1435:1476	Graphical abstractSchematic representation of simultaneous detecting serotonin (5-HT), dopamine (DA) and ascorbic acid (AA) for three-dimensional reduced-graphene oxide/Fe3O4/hydroxypropyl-β-cyclodextrin (3D-rGO/Fe3O4/HP-β-CD) by differential pulse voltammetry (DPV) approach.	1435:1710	Graphical abstractSchematic representation of simultaneous detecting serotonin (5-HT), dopamine (DA) and ascorbic acid (AA) for three-dimensional reduced-graphene oxide/Fe3O4/hydroxypropyl-β-cyclodextrin (3D-rGO/Fe3O4/HP-β-CD) by differential pulse voltammetry (DPV) approach.
31701250	6	36	theme	recoveries	1288:1297	arg1	limits					1233:1238	(c) detection limits	1219:1238	(c) detection limits of 3.3 nM, 6.7 nM and 3.3 μM (S/N = 3), and (d) recoveries of 96.9-103%, 97.3%-102% and 96.3-105% from spiked serum samples, respectively	1219:1376	The following figures of merit were found for simultaneous electrochemical determination of 5-HT, DA, and AA: (a) Well separated peaks at around 0.316, 0.16 and - 0.044 V; (b) linear responses in the 0.01 - 25 μM, 0.02 - 25 μM and 10 - 350 μM; (c) detection limits of 3.3 nM, 6.7 nM and 3.3 μM (S/N = 3), and (d) recoveries of 96.9-103%, 97.3%-102% and 96.3-105% from spiked serum samples, respectively.
31701250	0	37	theme	graphene	125:132	arg1	oxide					134:138	reduced graphene oxide	117:138	reduced graphene oxide	117:138	Simultaneous electrochemical sensing of serotonin, dopamine and ascorbic acid by using a nanocomposite prepared from reduced graphene oxide, Fe3O4 and hydroxypropyl-β-cyclodextrin.
31701250	7	38	theme	relative	1383:1390	arg1	RSD					1412:1414	RSD	1412:1414	RSD	1412:1414	All relative standard deviation (RSD) are less than 4%.
31701250	7	38	theme	relative	1383:1390	arg1	%					1432:1432	less than 4%	1421:1432	less than 4%	1421:1432	All relative standard deviation (RSD) are less than 4%.
31701250	7	38	theme	relative	1383:1390	arg1	deviation					1401:1409	All relative standard deviation	1379:1409	All relative standard deviation (RSD)	1379:1415	All relative standard deviation (RSD) are less than 4%.
31701250	3	39	theme	interconnected	538:551	arg1	framework					583:591	The interconnected porous reduced graphene oxide framework	534:591	The interconnected porous reduced graphene oxide framework tightly anchored to the Fe3O4 magnetic nanoparticles	534:644	The interconnected porous reduced graphene oxide framework tightly anchored to the Fe3O4 magnetic nanoparticles warrants good electrical conductivity and efficient catalytic activity.
31701250	6	40	theme	spiked	1343:1348	arg1	samples					1356:1362	spiked serum samples	1343:1362	spiked serum samples	1343:1362	The following figures of merit were found for simultaneous electrochemical determination of 5-HT, DA, and AA: (a) Well separated peaks at around 0.316, 0.16 and - 0.044 V; (b) linear responses in the 0.01 - 25 μM, 0.02 - 25 μM and 10 - 350 μM; (c) detection limits of 3.3 nM, 6.7 nM and 3.3 μM (S/N = 3), and (d) recoveries of 96.9-103%, 97.3%-102% and 96.3-105% from spiked serum samples, respectively.
31701250	1	41	theme	Fe3O4	215:219	arg1	nanoparticles					221:233	Fe3O4 nanoparticles	215:233	Fe3O4 nanoparticles	215:233	Reduced graphene oxide containing Fe3O4 nanoparticles was decorated with hydroxypropyl-β-cyclodextrin (HP-β-CD) to construct a novel nanocomposite (3D-rGO/Fe3O4/HP-β-CD).
31701250	5	42	theme	pulse	950:954	arg1	voltammetry					956:966	differential pulse voltammetry	937:966	differential pulse voltammetry (DPV)	937:972	Well-separated oxidation peaks and increased peak currents were observed for 5-HT, DA, and AA individually and in mixtures by differential pulse voltammetry (DPV).
31701250	5	42	theme	pulse	950:954	arg1	DPV					969:971	DPV	969:971	DPV	969:971	Well-separated oxidation peaks and increased peak currents were observed for 5-HT, DA, and AA individually and in mixtures by differential pulse voltammetry (DPV).
31701250	0	43	theme	reduced	117:123	arg1	oxide					134:138	reduced graphene oxide	117:138	reduced graphene oxide	117:138	Simultaneous electrochemical sensing of serotonin, dopamine and ascorbic acid by using a nanocomposite prepared from reduced graphene oxide, Fe3O4 and hydroxypropyl-β-cyclodextrin.
31701250	1	44	contain	containing	204:213	arg1	oxide					198:202	Reduced graphene oxide	181:202	Reduced graphene oxide containing Fe3O4 nanoparticles	181:233	Reduced graphene oxide containing Fe3O4 nanoparticles was decorated with hydroxypropyl-β-cyclodextrin (HP-β-CD) to construct a novel nanocomposite (3D-rGO/Fe3O4/HP-β-CD).
31701250	1	44	contain	containing	204:213	arg2	nanoparticles					221:233	Fe3O4 nanoparticles	215:233	Fe3O4 nanoparticles	215:233	Reduced graphene oxide containing Fe3O4 nanoparticles was decorated with hydroxypropyl-β-cyclodextrin (HP-β-CD) to construct a novel nanocomposite (3D-rGO/Fe3O4/HP-β-CD).
31701250	3	45	theme	porous	553:558	arg1	framework					583:591	The interconnected porous reduced graphene oxide framework	534:591	The interconnected porous reduced graphene oxide framework tightly anchored to the Fe3O4 magnetic nanoparticles	534:644	The interconnected porous reduced graphene oxide framework tightly anchored to the Fe3O4 magnetic nanoparticles warrants good electrical conductivity and efficient catalytic activity.
31701250	8	46	theme	ascorbic	1540:1547	arg1	AA					1555:1556	AA	1555:1556	AA	1555:1556	Graphical abstractSchematic representation of simultaneous detecting serotonin (5-HT), dopamine (DA) and ascorbic acid (AA) for three-dimensional reduced-graphene oxide/Fe3O4/hydroxypropyl-β-cyclodextrin (3D-rGO/Fe3O4/HP-β-CD) by differential pulse voltammetry (DPV) approach.
31701250	8	46	theme	ascorbic	1540:1547	arg1	acid					1549:1552	ascorbic acid	1540:1552	ascorbic acid (AA)	1540:1557	Graphical abstractSchematic representation of simultaneous detecting serotonin (5-HT), dopamine (DA) and ascorbic acid (AA) for three-dimensional reduced-graphene oxide/Fe3O4/hydroxypropyl-β-cyclodextrin (3D-rGO/Fe3O4/HP-β-CD) by differential pulse voltammetry (DPV) approach.
31701250	6	47	theme	d	1285:1285	arg1	recoveries					1288:1297	(d) recoveries	1284:1297	(d) recoveries	1284:1297	The following figures of merit were found for simultaneous electrochemical determination of 5-HT, DA, and AA: (a) Well separated peaks at around 0.316, 0.16 and - 0.044 V; (b) linear responses in the 0.01 - 25 μM, 0.02 - 25 μM and 10 - 350 μM; (c) detection limits of 3.3 nM, 6.7 nM and 3.3 μM (S/N = 3), and (d) recoveries of 96.9-103%, 97.3%-102% and 96.3-105% from spiked serum samples, respectively.
31701250	6	48	theme	separated	1094:1102	arg1	peaks					1104:1108	(a) Well separated peaks	1085:1108	(a) Well separated peaks at around 0.316, 0.16 and - 0.044 V	1085:1144	The following figures of merit were found for simultaneous electrochemical determination of 5-HT, DA, and AA: (a) Well separated peaks at around 0.316, 0.16 and - 0.044 V; (b) linear responses in the 0.01 - 25 μM, 0.02 - 25 μM and 10 - 350 μM; (c) detection limits of 3.3 nM, 6.7 nM and 3.3 μM (S/N = 3), and (d) recoveries of 96.9-103%, 97.3%-102% and 96.3-105% from spiked serum samples, respectively.
31701250	6	49	theme	detection	1223:1231	arg1	limits					1233:1238	(c) detection limits	1219:1238	(c) detection limits of 3.3 nM, 6.7 nM and 3.3 μM (S/N = 3), and (d) recoveries of 96.9-103%, 97.3%-102% and 96.3-105% from spiked serum samples, respectively	1219:1376	The following figures of merit were found for simultaneous electrochemical determination of 5-HT, DA, and AA: (a) Well separated peaks at around 0.316, 0.16 and - 0.044 V; (b) linear responses in the 0.01 - 25 μM, 0.02 - 25 μM and 10 - 350 μM; (c) detection limits of 3.3 nM, 6.7 nM and 3.3 μM (S/N = 3), and (d) recoveries of 96.9-103%, 97.3%-102% and 96.3-105% from spiked serum samples, respectively.
31701250	8	50	theme	acid	1549:1552	arg1	representation					1463:1476	Graphical abstractSchematic representation	1435:1476	Graphical abstractSchematic representation of simultaneous detecting serotonin (5-HT), dopamine (DA) and ascorbic acid (AA) for three-dimensional reduced-graphene oxide/Fe3O4/hydroxypropyl-β-cyclodextrin (3D-rGO/Fe3O4/HP-β-CD) by differential pulse voltammetry (DPV) approach.	1435:1710	Graphical abstractSchematic representation of simultaneous detecting serotonin (5-HT), dopamine (DA) and ascorbic acid (AA) for three-dimensional reduced-graphene oxide/Fe3O4/hydroxypropyl-β-cyclodextrin (3D-rGO/Fe3O4/HP-β-CD) by differential pulse voltammetry (DPV) approach.
31701250	6	51	theme	%	1336:1336	arg1	6.7 nM					1251:1256	6.7 nM	1251:1256	6.7 nM	1251:1256	The following figures of merit were found for simultaneous electrochemical determination of 5-HT, DA, and AA: (a) Well separated peaks at around 0.316, 0.16 and - 0.044 V; (b) linear responses in the 0.01 - 25 μM, 0.02 - 25 μM and 10 - 350 μM; (c) detection limits of 3.3 nM, 6.7 nM and 3.3 μM (S/N = 3), and (d) recoveries of 96.9-103%, 97.3%-102% and 96.3-105% from spiked serum samples, respectively.
31701250	6	51	theme	%	1336:1336	arg1	recoveries					1288:1297	(d) recoveries	1284:1297	(d) recoveries	1284:1297	The following figures of merit were found for simultaneous electrochemical determination of 5-HT, DA, and AA: (a) Well separated peaks at around 0.316, 0.16 and - 0.044 V; (b) linear responses in the 0.01 - 25 μM, 0.02 - 25 μM and 10 - 350 μM; (c) detection limits of 3.3 nM, 6.7 nM and 3.3 μM (S/N = 3), and (d) recoveries of 96.9-103%, 97.3%-102% and 96.3-105% from spiked serum samples, respectively.
31701250	6	51	theme	%	1336:1336	arg1	3.3 nM					1243:1248	3.3 nM	1243:1248	3.3 nM	1243:1248	The following figures of merit were found for simultaneous electrochemical determination of 5-HT, DA, and AA: (a) Well separated peaks at around 0.316, 0.16 and - 0.044 V; (b) linear responses in the 0.01 - 25 μM, 0.02 - 25 μM and 10 - 350 μM; (c) detection limits of 3.3 nM, 6.7 nM and 3.3 μM (S/N = 3), and (d) recoveries of 96.9-103%, 97.3%-102% and 96.3-105% from spiked serum samples, respectively.
31701250	6	51	theme	%	1336:1336	arg1	S/N = 3					1270:1276	S/N = 3	1270:1276	S/N = 3	1270:1276	The following figures of merit were found for simultaneous electrochemical determination of 5-HT, DA, and AA: (a) Well separated peaks at around 0.316, 0.16 and - 0.044 V; (b) linear responses in the 0.01 - 25 μM, 0.02 - 25 μM and 10 - 350 μM; (c) detection limits of 3.3 nM, 6.7 nM and 3.3 μM (S/N = 3), and (d) recoveries of 96.9-103%, 97.3%-102% and 96.3-105% from spiked serum samples, respectively.
31701250	6	51	theme	%	1336:1336	arg1	3.3 μM					1262:1267	3.3 μM	1262:1267	3.3 μM (S/N = 3)	1262:1277	The following figures of merit were found for simultaneous electrochemical determination of 5-HT, DA, and AA: (a) Well separated peaks at around 0.316, 0.16 and - 0.044 V; (b) linear responses in the 0.01 - 25 μM, 0.02 - 25 μM and 10 - 350 μM; (c) detection limits of 3.3 nM, 6.7 nM and 3.3 μM (S/N = 3), and (d) recoveries of 96.9-103%, 97.3%-102% and 96.3-105% from spiked serum samples, respectively.
31701250	2	52	theme	electrochemical	425:439	arg1	sensor					441:446	an electrochemical sensor	422:446	an electrochemical sensor for detecting simultaneously serotonin (5-HT), dopamine (DA), and ascorbic acid (AA)	422:531	The composite was placed on a glassy carbon electrode (GCE) to design an electrochemical sensor for detecting simultaneously serotonin (5-HT), dopamine (DA), and ascorbic acid (AA).
31701250	0	53	theme	serotonin	40:48	arg1	sensing					29:35	Simultaneous electrochemical sensing	0:35	Simultaneous electrochemical sensing of serotonin, dopamine and ascorbic acid by using a nanocomposite prepared from reduced graphene oxide, Fe3O4 and hydroxypropyl-β-cyclodextrin.	0:179	Simultaneous electrochemical sensing of serotonin, dopamine and ascorbic acid by using a nanocomposite prepared from reduced graphene oxide, Fe3O4 and hydroxypropyl-β-cyclodextrin.
31701250	6	54	theme	3.3 nM	1243:1248	arg1	limits					1233:1238	(c) detection limits	1219:1238	(c) detection limits of 3.3 nM, 6.7 nM and 3.3 μM (S/N = 3), and (d) recoveries of 96.9-103%, 97.3%-102% and 96.3-105% from spiked serum samples, respectively	1219:1376	The following figures of merit were found for simultaneous electrochemical determination of 5-HT, DA, and AA: (a) Well separated peaks at around 0.316, 0.16 and - 0.044 V; (b) linear responses in the 0.01 - 25 μM, 0.02 - 25 μM and 10 - 350 μM; (c) detection limits of 3.3 nM, 6.7 nM and 3.3 μM (S/N = 3), and (d) recoveries of 96.9-103%, 97.3%-102% and 96.3-105% from spiked serum samples, respectively.
31701250	6	55	theme	electrochemical	1034:1048	arg1	determination					1050:1062	simultaneous electrochemical determination	1021:1062	simultaneous electrochemical determination of 5-HT, DA, and AA	1021:1082	The following figures of merit were found for simultaneous electrochemical determination of 5-HT, DA, and AA: (a) Well separated peaks at around 0.316, 0.16 and - 0.044 V; (b) linear responses in the 0.01 - 25 μM, 0.02 - 25 μM and 10 - 350 μM; (c) detection limits of 3.3 nM, 6.7 nM and 3.3 μM (S/N = 3), and (d) recoveries of 96.9-103%, 97.3%-102% and 96.3-105% from spiked serum samples, respectively.
31701250	3	56	theme	good	655:658	arg1	conductivity					671:682	good electrical conductivity	655:682	good electrical conductivity	655:682	The interconnected porous reduced graphene oxide framework tightly anchored to the Fe3O4 magnetic nanoparticles warrants good electrical conductivity and efficient catalytic activity.
31701250	3	57	theme	oxide	577:581	arg1	framework					583:591	The interconnected porous reduced graphene oxide framework	534:591	The interconnected porous reduced graphene oxide framework tightly anchored to the Fe3O4 magnetic nanoparticles	534:644	The interconnected porous reduced graphene oxide framework tightly anchored to the Fe3O4 magnetic nanoparticles warrants good electrical conductivity and efficient catalytic activity.
31701250	6	58	dep	limits	1233:1238	arg1	c					1220:1220	c	1220:1220	c	1220:1220	The following figures of merit were found for simultaneous electrochemical determination of 5-HT, DA, and AA: (a) Well separated peaks at around 0.316, 0.16 and - 0.044 V; (b) linear responses in the 0.01 - 25 μM, 0.02 - 25 μM and 10 - 350 μM; (c) detection limits of 3.3 nM, 6.7 nM and 3.3 μM (S/N = 3), and (d) recoveries of 96.9-103%, 97.3%-102% and 96.3-105% from spiked serum samples, respectively.
31701250	0	59	theme	dopamine	51:58	arg1	sensing					29:35	Simultaneous electrochemical sensing	0:35	Simultaneous electrochemical sensing of serotonin, dopamine and ascorbic acid by using a nanocomposite prepared from reduced graphene oxide, Fe3O4 and hydroxypropyl-β-cyclodextrin.	0:179	Simultaneous electrochemical sensing of serotonin, dopamine and ascorbic acid by using a nanocomposite prepared from reduced graphene oxide, Fe3O4 and hydroxypropyl-β-cyclodextrin.
31701250	3	60	theme	catalytic	698:706	arg1	activity					708:715	efficient catalytic activity	688:715	efficient catalytic activity	688:715	The interconnected porous reduced graphene oxide framework tightly anchored to the Fe3O4 magnetic nanoparticles warrants good electrical conductivity and efficient catalytic activity.
31701250	8	61	theme	three-dimensional	1563:1579	arg1	oxide/Fe3O4/hydroxypropyl-β-cyclodextrin					1598:1637	three-dimensional reduced-graphene oxide/Fe3O4/hydroxypropyl-β-cyclodextrin	1563:1637	three-dimensional reduced-graphene oxide/Fe3O4/hydroxypropyl-β-cyclodextrin (3D-rGO/Fe3O4/HP-β-CD) by differential pulse voltammetry (DPV) approach	1563:1709	Graphical abstractSchematic representation of simultaneous detecting serotonin (5-HT), dopamine (DA) and ascorbic acid (AA) for three-dimensional reduced-graphene oxide/Fe3O4/hydroxypropyl-β-cyclodextrin (3D-rGO/Fe3O4/HP-β-CD) by differential pulse voltammetry (DPV) approach.
31701250	8	61	theme	three-dimensional	1563:1579	arg1	3D-rGO/Fe3O4/HP-β-CD					1640:1659	3D-rGO/Fe3O4/HP-β-CD	1640:1659	3D-rGO/Fe3O4/HP-β-CD	1640:1659	Graphical abstractSchematic representation of simultaneous detecting serotonin (5-HT), dopamine (DA) and ascorbic acid (AA) for three-dimensional reduced-graphene oxide/Fe3O4/hydroxypropyl-β-cyclodextrin (3D-rGO/Fe3O4/HP-β-CD) by differential pulse voltammetry (DPV) approach.
31701250	6	62	theme	%	1310:1310	arg1	6.7 nM					1251:1256	6.7 nM	1251:1256	6.7 nM	1251:1256	The following figures of merit were found for simultaneous electrochemical determination of 5-HT, DA, and AA: (a) Well separated peaks at around 0.316, 0.16 and - 0.044 V; (b) linear responses in the 0.01 - 25 μM, 0.02 - 25 μM and 10 - 350 μM; (c) detection limits of 3.3 nM, 6.7 nM and 3.3 μM (S/N = 3), and (d) recoveries of 96.9-103%, 97.3%-102% and 96.3-105% from spiked serum samples, respectively.
31701250	6	62	theme	%	1310:1310	arg1	recoveries					1288:1297	(d) recoveries	1284:1297	(d) recoveries	1284:1297	The following figures of merit were found for simultaneous electrochemical determination of 5-HT, DA, and AA: (a) Well separated peaks at around 0.316, 0.16 and - 0.044 V; (b) linear responses in the 0.01 - 25 μM, 0.02 - 25 μM and 10 - 350 μM; (c) detection limits of 3.3 nM, 6.7 nM and 3.3 μM (S/N = 3), and (d) recoveries of 96.9-103%, 97.3%-102% and 96.3-105% from spiked serum samples, respectively.
31701250	6	62	theme	%	1310:1310	arg1	3.3 nM					1243:1248	3.3 nM	1243:1248	3.3 nM	1243:1248	The following figures of merit were found for simultaneous electrochemical determination of 5-HT, DA, and AA: (a) Well separated peaks at around 0.316, 0.16 and - 0.044 V; (b) linear responses in the 0.01 - 25 μM, 0.02 - 25 μM and 10 - 350 μM; (c) detection limits of 3.3 nM, 6.7 nM and 3.3 μM (S/N = 3), and (d) recoveries of 96.9-103%, 97.3%-102% and 96.3-105% from spiked serum samples, respectively.
31701250	6	62	theme	%	1310:1310	arg1	S/N = 3					1270:1276	S/N = 3	1270:1276	S/N = 3	1270:1276	The following figures of merit were found for simultaneous electrochemical determination of 5-HT, DA, and AA: (a) Well separated peaks at around 0.316, 0.16 and - 0.044 V; (b) linear responses in the 0.01 - 25 μM, 0.02 - 25 μM and 10 - 350 μM; (c) detection limits of 3.3 nM, 6.7 nM and 3.3 μM (S/N = 3), and (d) recoveries of 96.9-103%, 97.3%-102% and 96.3-105% from spiked serum samples, respectively.
31701250	6	62	theme	%	1310:1310	arg1	3.3 μM					1262:1267	3.3 μM	1262:1267	3.3 μM (S/N = 3)	1262:1277	The following figures of merit were found for simultaneous electrochemical determination of 5-HT, DA, and AA: (a) Well separated peaks at around 0.316, 0.16 and - 0.044 V; (b) linear responses in the 0.01 - 25 μM, 0.02 - 25 μM and 10 - 350 μM; (c) detection limits of 3.3 nM, 6.7 nM and 3.3 μM (S/N = 3), and (d) recoveries of 96.9-103%, 97.3%-102% and 96.3-105% from spiked serum samples, respectively.
31701250	8	63	theme	simultaneous	1481:1492	arg1	5-HT					1515:1518	5-HT	1515:1518	5-HT	1515:1518	Graphical abstractSchematic representation of simultaneous detecting serotonin (5-HT), dopamine (DA) and ascorbic acid (AA) for three-dimensional reduced-graphene oxide/Fe3O4/hydroxypropyl-β-cyclodextrin (3D-rGO/Fe3O4/HP-β-CD) by differential pulse voltammetry (DPV) approach.
31701250	8	63	theme	simultaneous	1481:1492	arg1	serotonin					1504:1512	simultaneous detecting serotonin	1481:1512	simultaneous detecting serotonin (5-HT)	1481:1519	Graphical abstractSchematic representation of simultaneous detecting serotonin (5-HT), dopamine (DA) and ascorbic acid (AA) for three-dimensional reduced-graphene oxide/Fe3O4/hydroxypropyl-β-cyclodextrin (3D-rGO/Fe3O4/HP-β-CD) by differential pulse voltammetry (DPV) approach.
31701250	6	64	theme	b	1148:1148	arg1	responses					1158:1166	(b) linear responses	1147:1166	(b) linear responses in the 0.01 - 25 μM, 0.02 - 25 μM and 10 - 350 μM	1147:1216	The following figures of merit were found for simultaneous electrochemical determination of 5-HT, DA, and AA: (a) Well separated peaks at around 0.316, 0.16 and - 0.044 V; (b) linear responses in the 0.01 - 25 μM, 0.02 - 25 μM and 10 - 350 μM; (c) detection limits of 3.3 nM, 6.7 nM and 3.3 μM (S/N = 3), and (d) recoveries of 96.9-103%, 97.3%-102% and 96.3-105% from spiked serum samples, respectively.
31701250	6	65	dep	0.01	1175:1178	arg1	the					1171:1173	the	1171:1173	the	1171:1173	The following figures of merit were found for simultaneous electrochemical determination of 5-HT, DA, and AA: (a) Well separated peaks at around 0.316, 0.16 and - 0.044 V; (b) linear responses in the 0.01 - 25 μM, 0.02 - 25 μM and 10 - 350 μM; (c) detection limits of 3.3 nM, 6.7 nM and 3.3 μM (S/N = 3), and (d) recoveries of 96.9-103%, 97.3%-102% and 96.3-105% from spiked serum samples, respectively.
31701250	3	66	theme	magnetic	623:630	arg1	nanoparticles					632:644	the Fe3O4 magnetic nanoparticles	613:644	the Fe3O4 magnetic nanoparticles	613:644	The interconnected porous reduced graphene oxide framework tightly anchored to the Fe3O4 magnetic nanoparticles warrants good electrical conductivity and efficient catalytic activity.
31701250	8	67	theme	reduced-graphene	1581:1596	arg1	oxide/Fe3O4/hydroxypropyl-β-cyclodextrin					1598:1637	three-dimensional reduced-graphene oxide/Fe3O4/hydroxypropyl-β-cyclodextrin	1563:1637	three-dimensional reduced-graphene oxide/Fe3O4/hydroxypropyl-β-cyclodextrin (3D-rGO/Fe3O4/HP-β-CD) by differential pulse voltammetry (DPV) approach	1563:1709	Graphical abstractSchematic representation of simultaneous detecting serotonin (5-HT), dopamine (DA) and ascorbic acid (AA) for three-dimensional reduced-graphene oxide/Fe3O4/hydroxypropyl-β-cyclodextrin (3D-rGO/Fe3O4/HP-β-CD) by differential pulse voltammetry (DPV) approach.
31701250	8	67	theme	reduced-graphene	1581:1596	arg1	3D-rGO/Fe3O4/HP-β-CD					1640:1659	3D-rGO/Fe3O4/HP-β-CD	1640:1659	3D-rGO/Fe3O4/HP-β-CD	1640:1659	Graphical abstractSchematic representation of simultaneous detecting serotonin (5-HT), dopamine (DA) and ascorbic acid (AA) for three-dimensional reduced-graphene oxide/Fe3O4/hydroxypropyl-β-cyclodextrin (3D-rGO/Fe3O4/HP-β-CD) by differential pulse voltammetry (DPV) approach.
31701250	8	68	theme	abstractSchematic	1445:1461	arg1	representation					1463:1476	Graphical abstractSchematic representation	1435:1476	Graphical abstractSchematic representation of simultaneous detecting serotonin (5-HT), dopamine (DA) and ascorbic acid (AA) for three-dimensional reduced-graphene oxide/Fe3O4/hydroxypropyl-β-cyclodextrin (3D-rGO/Fe3O4/HP-β-CD) by differential pulse voltammetry (DPV) approach.	1435:1710	Graphical abstractSchematic representation of simultaneous detecting serotonin (5-HT), dopamine (DA) and ascorbic acid (AA) for three-dimensional reduced-graphene oxide/Fe3O4/hydroxypropyl-β-cyclodextrin (3D-rGO/Fe3O4/HP-β-CD) by differential pulse voltammetry (DPV) approach.
31701250	8	69	theme	dopamine	1522:1529	arg1	representation					1463:1476	Graphical abstractSchematic representation	1435:1476	Graphical abstractSchematic representation of simultaneous detecting serotonin (5-HT), dopamine (DA) and ascorbic acid (AA) for three-dimensional reduced-graphene oxide/Fe3O4/hydroxypropyl-β-cyclodextrin (3D-rGO/Fe3O4/HP-β-CD) by differential pulse voltammetry (DPV) approach.	1435:1710	Graphical abstractSchematic representation of simultaneous detecting serotonin (5-HT), dopamine (DA) and ascorbic acid (AA) for three-dimensional reduced-graphene oxide/Fe3O4/hydroxypropyl-β-cyclodextrin (3D-rGO/Fe3O4/HP-β-CD) by differential pulse voltammetry (DPV) approach.
31701250	6	70	theme	simultaneous	1021:1032	arg1	determination					1050:1062	simultaneous electrochemical determination	1021:1062	simultaneous electrochemical determination of 5-HT, DA, and AA	1021:1082	The following figures of merit were found for simultaneous electrochemical determination of 5-HT, DA, and AA: (a) Well separated peaks at around 0.316, 0.16 and - 0.044 V; (b) linear responses in the 0.01 - 25 μM, 0.02 - 25 μM and 10 - 350 μM; (c) detection limits of 3.3 nM, 6.7 nM and 3.3 μM (S/N = 3), and (d) recoveries of 96.9-103%, 97.3%-102% and 96.3-105% from spiked serum samples, respectively.
31701250	5	71	theme	increased	846:854	arg1	currents					861:868	increased peak currents	846:868	increased peak currents	846:868	Well-separated oxidation peaks and increased peak currents were observed for 5-HT, DA, and AA individually and in mixtures by differential pulse voltammetry (DPV).
31701250	4	72	theme	supramolecular	740:753	arg1	HP-β-CD					722:728	The HP-β-CD	718:728	The HP-β-CD	718:728	The HP-β-CD acts as a supramolecular host with high recognition ability for 5-HT, DA and AA.
31701250	4	72	theme	supramolecular	740:753	arg1	host					755:758	a supramolecular host	738:758	a supramolecular host with high recognition ability for 5-HT, DA and AA	738:808	The HP-β-CD acts as a supramolecular host with high recognition ability for 5-HT, DA and AA.
31701250	6	73	theme	%	1322:1322	arg1	6.7 nM					1251:1256	6.7 nM	1251:1256	6.7 nM	1251:1256	The following figures of merit were found for simultaneous electrochemical determination of 5-HT, DA, and AA: (a) Well separated peaks at around 0.316, 0.16 and - 0.044 V; (b) linear responses in the 0.01 - 25 μM, 0.02 - 25 μM and 10 - 350 μM; (c) detection limits of 3.3 nM, 6.7 nM and 3.3 μM (S/N = 3), and (d) recoveries of 96.9-103%, 97.3%-102% and 96.3-105% from spiked serum samples, respectively.
31701250	6	73	theme	%	1322:1322	arg1	recoveries					1288:1297	(d) recoveries	1284:1297	(d) recoveries	1284:1297	The following figures of merit were found for simultaneous electrochemical determination of 5-HT, DA, and AA: (a) Well separated peaks at around 0.316, 0.16 and - 0.044 V; (b) linear responses in the 0.01 - 25 μM, 0.02 - 25 μM and 10 - 350 μM; (c) detection limits of 3.3 nM, 6.7 nM and 3.3 μM (S/N = 3), and (d) recoveries of 96.9-103%, 97.3%-102% and 96.3-105% from spiked serum samples, respectively.
31701250	6	73	theme	%	1322:1322	arg1	3.3 nM					1243:1248	3.3 nM	1243:1248	3.3 nM	1243:1248	The following figures of merit were found for simultaneous electrochemical determination of 5-HT, DA, and AA: (a) Well separated peaks at around 0.316, 0.16 and - 0.044 V; (b) linear responses in the 0.01 - 25 μM, 0.02 - 25 μM and 10 - 350 μM; (c) detection limits of 3.3 nM, 6.7 nM and 3.3 μM (S/N = 3), and (d) recoveries of 96.9-103%, 97.3%-102% and 96.3-105% from spiked serum samples, respectively.
31701250	6	73	theme	%	1322:1322	arg1	S/N = 3					1270:1276	S/N = 3	1270:1276	S/N = 3	1270:1276	The following figures of merit were found for simultaneous electrochemical determination of 5-HT, DA, and AA: (a) Well separated peaks at around 0.316, 0.16 and - 0.044 V; (b) linear responses in the 0.01 - 25 μM, 0.02 - 25 μM and 10 - 350 μM; (c) detection limits of 3.3 nM, 6.7 nM and 3.3 μM (S/N = 3), and (d) recoveries of 96.9-103%, 97.3%-102% and 96.3-105% from spiked serum samples, respectively.
31701250	6	73	theme	%	1322:1322	arg1	3.3 μM					1262:1267	3.3 μM	1262:1267	3.3 μM (S/N = 3)	1262:1277	The following figures of merit were found for simultaneous electrochemical determination of 5-HT, DA, and AA: (a) Well separated peaks at around 0.316, 0.16 and - 0.044 V; (b) linear responses in the 0.01 - 25 μM, 0.02 - 25 μM and 10 - 350 μM; (c) detection limits of 3.3 nM, 6.7 nM and 3.3 μM (S/N = 3), and (d) recoveries of 96.9-103%, 97.3%-102% and 96.3-105% from spiked serum samples, respectively.
31701250	0	74	theme	acid	73:76	arg1	sensing					29:35	Simultaneous electrochemical sensing	0:35	Simultaneous electrochemical sensing of serotonin, dopamine and ascorbic acid by using a nanocomposite prepared from reduced graphene oxide, Fe3O4 and hydroxypropyl-β-cyclodextrin.	0:179	Simultaneous electrochemical sensing of serotonin, dopamine and ascorbic acid by using a nanocomposite prepared from reduced graphene oxide, Fe3O4 and hydroxypropyl-β-cyclodextrin.
31701250	3	75	theme	efficient	688:696	arg1	activity					708:715	efficient catalytic activity	688:715	efficient catalytic activity	688:715	The interconnected porous reduced graphene oxide framework tightly anchored to the Fe3O4 magnetic nanoparticles warrants good electrical conductivity and efficient catalytic activity.
31701250	6	76	dep	peaks	1104:1108	arg1	a					1086:1086	a	1086:1086	a	1086:1086	The following figures of merit were found for simultaneous electrochemical determination of 5-HT, DA, and AA: (a) Well separated peaks at around 0.316, 0.16 and - 0.044 V; (b) linear responses in the 0.01 - 25 μM, 0.02 - 25 μM and 10 - 350 μM; (c) detection limits of 3.3 nM, 6.7 nM and 3.3 μM (S/N = 3), and (d) recoveries of 96.9-103%, 97.3%-102% and 96.3-105% from spiked serum samples, respectively.
31701250	5	77	theme	peak	856:859	arg1	currents					861:868	increased peak currents	846:868	increased peak currents	846:868	Well-separated oxidation peaks and increased peak currents were observed for 5-HT, DA, and AA individually and in mixtures by differential pulse voltammetry (DPV).
31701250	8	78	theme	detecting	1494:1502	arg1	5-HT					1515:1518	5-HT	1515:1518	5-HT	1515:1518	Graphical abstractSchematic representation of simultaneous detecting serotonin (5-HT), dopamine (DA) and ascorbic acid (AA) for three-dimensional reduced-graphene oxide/Fe3O4/hydroxypropyl-β-cyclodextrin (3D-rGO/Fe3O4/HP-β-CD) by differential pulse voltammetry (DPV) approach.
31701250	8	78	theme	detecting	1494:1502	arg1	serotonin					1504:1512	simultaneous detecting serotonin	1481:1512	simultaneous detecting serotonin (5-HT)	1481:1519	Graphical abstractSchematic representation of simultaneous detecting serotonin (5-HT), dopamine (DA) and ascorbic acid (AA) for three-dimensional reduced-graphene oxide/Fe3O4/hydroxypropyl-β-cyclodextrin (3D-rGO/Fe3O4/HP-β-CD) by differential pulse voltammetry (DPV) approach.
31701250	0	79	theme	ascorbic	64:71	arg1	acid					73:76	ascorbic acid	64:76	ascorbic acid	64:76	Simultaneous electrochemical sensing of serotonin, dopamine and ascorbic acid by using a nanocomposite prepared from reduced graphene oxide, Fe3O4 and hydroxypropyl-β-cyclodextrin.
31701250	4	80	with	host	755:758	arg1	ability					782:788	high recognition ability	765:788	high recognition ability	765:788	The HP-β-CD acts as a supramolecular host with high recognition ability for 5-HT, DA and AA.
31701250	3	81	theme	the Fe3O4	613:621	arg1	nanoparticles					632:644	the Fe3O4 magnetic nanoparticles	613:644	the Fe3O4 magnetic nanoparticles	613:644	The interconnected porous reduced graphene oxide framework tightly anchored to the Fe3O4 magnetic nanoparticles warrants good electrical conductivity and efficient catalytic activity.
31701250	5	82	theme	oxidation	826:834	arg1	peaks					836:840	Well-separated oxidation peaks	811:840	Well-separated oxidation peaks	811:840	Well-separated oxidation peaks and increased peak currents were observed for 5-HT, DA, and AA individually and in mixtures by differential pulse voltammetry (DPV).
31701250	6	83	theme	%	1317:1317	arg1	%					1322:1322	97.3%-102%	1313:1322	97.3%-102%	1313:1322	The following figures of merit were found for simultaneous electrochemical determination of 5-HT, DA, and AA: (a) Well separated peaks at around 0.316, 0.16 and - 0.044 V; (b) linear responses in the 0.01 - 25 μM, 0.02 - 25 μM and 10 - 350 μM; (c) detection limits of 3.3 nM, 6.7 nM and 3.3 μM (S/N = 3), and (d) recoveries of 96.9-103%, 97.3%-102% and 96.3-105% from spiked serum samples, respectively.
31701250	6	84	theme	6.7 nM	1251:1256	arg1	limits					1233:1238	(c) detection limits	1219:1238	(c) detection limits of 3.3 nM, 6.7 nM and 3.3 μM (S/N = 3), and (d) recoveries of 96.9-103%, 97.3%-102% and 96.3-105% from spiked serum samples, respectively	1219:1376	The following figures of merit were found for simultaneous electrochemical determination of 5-HT, DA, and AA: (a) Well separated peaks at around 0.316, 0.16 and - 0.044 V; (b) linear responses in the 0.01 - 25 μM, 0.02 - 25 μM and 10 - 350 μM; (c) detection limits of 3.3 nM, 6.7 nM and 3.3 μM (S/N = 3), and (d) recoveries of 96.9-103%, 97.3%-102% and 96.3-105% from spiked serum samples, respectively.
30952424	1	0	theme	biopharmaceutical	353:369	arg1	glycosylation					371:383	biopharmaceutical glycosylation	353:383	biopharmaceutical glycosylation	353:383	O-Linked glycan liberation from proteins through reductive beta-elimination and hydrazinolysis is widely used, but have yet to satisfy the recent needs for glycan analysis in glycan biomarker research and microheterogeneity evaluation of biopharmaceutical glycosylation.
30952424	1	1	theme	reductive	164:172	arg1	beta-elimination					174:189	reductive beta-elimination	164:189	reductive beta-elimination	164:189	O-Linked glycan liberation from proteins through reductive beta-elimination and hydrazinolysis is widely used, but have yet to satisfy the recent needs for glycan analysis in glycan biomarker research and microheterogeneity evaluation of biopharmaceutical glycosylation.
30952424	6	2	theme	porcine	969:975	arg1	mucins					990:995	porcine submaxillary mucins	969:995	porcine submaxillary mucins separated by supported molecular matrix electrophoresis (SMME) which is previously developed to characterize mucins	969:1111	To demonstrate the feasibility of the developed method, we analyzed O-linked glycans of porcine submaxillary mucins separated by supported molecular matrix electrophoresis (SMME) which is previously developed to characterize mucins.
30952424	1	3	theme	glycosylation	371:383	arg1	evaluation					339:348	evaluation	339:348	evaluation of biopharmaceutical glycosylation	339:383	O-Linked glycan liberation from proteins through reductive beta-elimination and hydrazinolysis is widely used, but have yet to satisfy the recent needs for glycan analysis in glycan biomarker research and microheterogeneity evaluation of biopharmaceutical glycosylation.
30952424	5	4	contain	containing	828:837	arg1	glycans					820:826	glycans	820:826	glycans containing NeuGc without degradation or deacylation	820:878	The optimized method also released glycans containing NeuGc without degradation or deacylation.
30952424	5	4	contain	containing	828:837	arg2	NeuGc					839:843	NeuGc	839:843	NeuGc	839:843	The optimized method also released glycans containing NeuGc without degradation or deacylation.
30952424	4	5	theme	optimized	716:724	arg1	liberation					742:751	the optimized O-linked glycan liberation	712:751	the optimized O-linked glycan liberation	712:751	In addition, we examined the compatibility of the optimized O-linked glycan liberation with denaturant and detergents.
30952424	6	6	theme	method	929:934	arg1	feasibility					900:910	the feasibility	896:910	the feasibility of the developed method	896:934	To demonstrate the feasibility of the developed method, we analyzed O-linked glycans of porcine submaxillary mucins separated by supported molecular matrix electrophoresis (SMME) which is previously developed to characterize mucins.
30952424	0	7	theme	organic	96:102	arg1	superbase					104:112	an organic superbase	93:112	an organic superbase	93:112	A practical method of liberating O-linked glycans from glycoproteins using hydroxylamine and an organic superbase.
30952424	0	8	gly	glycoproteins	55:67	arg1	glycoproteins					55:67	glycoproteins	55:67	glycoproteins using hydroxylamine and an organic superbase	55:112	A practical method of liberating O-linked glycans from glycoproteins using hydroxylamine and an organic superbase.
30952424	6	9	theme	O-linked	949:956	arg1	glycans					958:964	O-linked glycans	949:964	O-linked glycans of porcine submaxillary mucins separated by supported molecular matrix electrophoresis (SMME) which is previously developed to characterize mucins	949:1111	To demonstrate the feasibility of the developed method, we analyzed O-linked glycans of porcine submaxillary mucins separated by supported molecular matrix electrophoresis (SMME) which is previously developed to characterize mucins.
30952424	1	10	from	proteins	147:154	arg1	liberation					131:140	O-Linked glycan liberation	115:140	O-Linked glycan liberation from proteins through reductive beta-elimination and hydrazinolysis	115:208	O-Linked glycan liberation from proteins through reductive beta-elimination and hydrazinolysis is widely used, but have yet to satisfy the recent needs for glycan analysis in glycan biomarker research and microheterogeneity evaluation of biopharmaceutical glycosylation.
30952424	4	11	theme	O-linked	726:733	arg1	liberation					742:751	the optimized O-linked glycan liberation	712:751	the optimized O-linked glycan liberation	712:751	In addition, we examined the compatibility of the optimized O-linked glycan liberation with denaturant and detergents.
30952424	1	12	theme	glycan	271:276	arg1	analysis					278:285	glycan analysis	271:285	glycan analysis in glycan biomarker research and microheterogeneity evaluation of biopharmaceutical glycosylation	271:383	O-Linked glycan liberation from proteins through reductive beta-elimination and hydrazinolysis is widely used, but have yet to satisfy the recent needs for glycan analysis in glycan biomarker research and microheterogeneity evaluation of biopharmaceutical glycosylation.
30952424	2	13	theme	reaction	535:542	arg1	conditions					544:553	the reaction conditions	531:553	the reaction conditions	531:553	Here, we introduce an alternative method by using hydroxylamine and an organic superbase, 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU), and optimize the reaction conditions.
30952424	4	14	theme	liberation	742:751	arg1	compatibility					695:707	the compatibility	691:707	the compatibility of the optimized O-linked glycan liberation with denaturant and detergents	691:782	In addition, we examined the compatibility of the optimized O-linked glycan liberation with denaturant and detergents.
30952424	7	15	theme	glycan	1260:1265	arg1	analyses					1267:1274	O-linked glycan analyses	1251:1274	O-linked glycan analyses	1251:1274	The method for O-linked glycan liberation and fluorescent labeling presented here was easy and rapid, and will be practically useful for O-linked glycan analyses.
30952424	1	16	dep	research	307:314	arg1	evaluation					339:348	evaluation	339:348	evaluation of biopharmaceutical glycosylation	339:383	O-Linked glycan liberation from proteins through reductive beta-elimination and hydrazinolysis is widely used, but have yet to satisfy the recent needs for glycan analysis in glycan biomarker research and microheterogeneity evaluation of biopharmaceutical glycosylation.
30952424	0	17	attach	liberating	22:31	arg1	glycoproteins					55:67	glycoproteins	55:67	glycoproteins using hydroxylamine and an organic superbase	55:112	A practical method of liberating O-linked glycans from glycoproteins using hydroxylamine and an organic superbase.
30952424	0	17	attach	liberating	22:31	arg2	method					12:17	A practical method	0:17	A practical method of liberating O-linked glycans from glycoproteins using hydroxylamine and an organic superbase.	0:113	A practical method of liberating O-linked glycans from glycoproteins using hydroxylamine and an organic superbase.
30952424	3	18	theme	developed	560:568	arg1	method					570:575	The developed method	556:575	The developed method	556:575	The developed method afforded comparable results to those of hydrazinolysis, but with less degraded products.
30952424	7	19	theme	O-linked	1251:1258	arg1	analyses					1267:1274	O-linked glycan analyses	1251:1274	O-linked glycan analyses	1251:1274	The method for O-linked glycan liberation and fluorescent labeling presented here was easy and rapid, and will be practically useful for O-linked glycan analyses.
30952424	0	20	theme	practical	2:10	arg1	method					12:17	A practical method	0:17	A practical method of liberating O-linked glycans from glycoproteins using hydroxylamine and an organic superbase.	0:113	A practical method of liberating O-linked glycans from glycoproteins using hydroxylamine and an organic superbase.
30952424	6	21	theme	molecular	1020:1028	arg1	SMME					1054:1057	SMME	1054:1057	SMME	1054:1057	To demonstrate the feasibility of the developed method, we analyzed O-linked glycans of porcine submaxillary mucins separated by supported molecular matrix electrophoresis (SMME) which is previously developed to characterize mucins.
30952424	6	21	theme	molecular	1020:1028	arg1	electrophoresis					1037:1051	supported molecular matrix electrophoresis	1010:1051	supported molecular matrix electrophoresis (SMME) which is previously developed to characterize mucins	1010:1111	To demonstrate the feasibility of the developed method, we analyzed O-linked glycans of porcine submaxillary mucins separated by supported molecular matrix electrophoresis (SMME) which is previously developed to characterize mucins.
30952424	1	22	from	analysis	278:285	arg1	microheterogeneity					320:337	microheterogeneity	320:337	microheterogeneity	320:337	O-Linked glycan liberation from proteins through reductive beta-elimination and hydrazinolysis is widely used, but have yet to satisfy the recent needs for glycan analysis in glycan biomarker research and microheterogeneity evaluation of biopharmaceutical glycosylation.
30952424	1	22	from	analysis	278:285	arg1	research					307:314	glycan biomarker research	290:314	glycan biomarker research	290:314	O-Linked glycan liberation from proteins through reductive beta-elimination and hydrazinolysis is widely used, but have yet to satisfy the recent needs for glycan analysis in glycan biomarker research and microheterogeneity evaluation of biopharmaceutical glycosylation.
30952424	4	23	link	O-linked	726:733	arg1	liberation					742:751	the optimized O-linked glycan liberation	712:751	the optimized O-linked glycan liberation	712:751	In addition, we examined the compatibility of the optimized O-linked glycan liberation with denaturant and detergents.
30952424	2	24	theme	alternative	408:418	arg1	method					420:425	an alternative method	405:425	an alternative method by using hydroxylamine and an organic superbase, 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU)	405:515	Here, we introduce an alternative method by using hydroxylamine and an organic superbase, 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU), and optimize the reaction conditions.
30952424	7	25	theme	fluorescent	1160:1170	arg1	labeling					1172:1179	fluorescent labeling	1160:1179	fluorescent labeling	1160:1179	The method for O-linked glycan liberation and fluorescent labeling presented here was easy and rapid, and will be practically useful for O-linked glycan analyses.
30952424	0	26	link	O-linked	33:40	arg1	glycans					42:48	O-linked glycans	33:48	O-linked glycans	33:48	A practical method of liberating O-linked glycans from glycoproteins using hydroxylamine and an organic superbase.
30952424	6	27	theme	matrix	1030:1035	arg1	SMME					1054:1057	SMME	1054:1057	SMME	1054:1057	To demonstrate the feasibility of the developed method, we analyzed O-linked glycans of porcine submaxillary mucins separated by supported molecular matrix electrophoresis (SMME) which is previously developed to characterize mucins.
30952424	6	27	theme	matrix	1030:1035	arg1	electrophoresis					1037:1051	supported molecular matrix electrophoresis	1010:1051	supported molecular matrix electrophoresis (SMME) which is previously developed to characterize mucins	1010:1111	To demonstrate the feasibility of the developed method, we analyzed O-linked glycans of porcine submaxillary mucins separated by supported molecular matrix electrophoresis (SMME) which is previously developed to characterize mucins.
30952424	3	28	theme	comparable	586:595	arg1	results					597:603	comparable results	586:603	comparable results	586:603	The developed method afforded comparable results to those of hydrazinolysis, but with less degraded products.
30952424	2	29	theme	organic	457:463	arg1	undec-7-ene					499:509	1,8-diazabicyclo[5.4.0]undec-7-ene	476:509	1,8-diazabicyclo[5.4.0]undec-7-ene (DBU)	476:515	Here, we introduce an alternative method by using hydroxylamine and an organic superbase, 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU), and optimize the reaction conditions.
30952424	2	29	theme	organic	457:463	arg1	superbase					465:473	an organic superbase	454:473	an organic superbase	454:473	Here, we introduce an alternative method by using hydroxylamine and an organic superbase, 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU), and optimize the reaction conditions.
30952424	1	30	theme	glycan	290:295	arg1	research					307:314	glycan biomarker research	290:314	glycan biomarker research	290:314	O-Linked glycan liberation from proteins through reductive beta-elimination and hydrazinolysis is widely used, but have yet to satisfy the recent needs for glycan analysis in glycan biomarker research and microheterogeneity evaluation of biopharmaceutical glycosylation.
30952424	4	31	with	compatibility	695:707	arg1	detergents					773:782	detergents	773:782	detergents	773:782	In addition, we examined the compatibility of the optimized O-linked glycan liberation with denaturant and detergents.
30952424	4	31	with	compatibility	695:707	arg1	denaturant					758:767	denaturant	758:767	denaturant	758:767	In addition, we examined the compatibility of the optimized O-linked glycan liberation with denaturant and detergents.
30952424	0	32	theme	O-linked	33:40	arg1	glycans					42:48	O-linked glycans	33:48	O-linked glycans	33:48	A practical method of liberating O-linked glycans from glycoproteins using hydroxylamine and an organic superbase.
30952424	1	33	theme	biomarker	297:305	arg1	research					307:314	glycan biomarker research	290:314	glycan biomarker research	290:314	O-Linked glycan liberation from proteins through reductive beta-elimination and hydrazinolysis is widely used, but have yet to satisfy the recent needs for glycan analysis in glycan biomarker research and microheterogeneity evaluation of biopharmaceutical glycosylation.
30952424	6	34	theme	developed	919:927	arg1	method					929:934	the developed method	915:934	the developed method	915:934	To demonstrate the feasibility of the developed method, we analyzed O-linked glycans of porcine submaxillary mucins separated by supported molecular matrix electrophoresis (SMME) which is previously developed to characterize mucins.
30952424	6	35	link	O-linked	949:956	arg1	glycans					958:964	O-linked glycans	949:964	O-linked glycans of porcine submaxillary mucins separated by supported molecular matrix electrophoresis (SMME) which is previously developed to characterize mucins	949:1111	To demonstrate the feasibility of the developed method, we analyzed O-linked glycans of porcine submaxillary mucins separated by supported molecular matrix electrophoresis (SMME) which is previously developed to characterize mucins.
30952424	6	36	theme	supported	1010:1018	arg1	SMME					1054:1057	SMME	1054:1057	SMME	1054:1057	To demonstrate the feasibility of the developed method, we analyzed O-linked glycans of porcine submaxillary mucins separated by supported molecular matrix electrophoresis (SMME) which is previously developed to characterize mucins.
30952424	6	36	theme	supported	1010:1018	arg1	electrophoresis					1037:1051	supported molecular matrix electrophoresis	1010:1051	supported molecular matrix electrophoresis (SMME) which is previously developed to characterize mucins	1010:1111	To demonstrate the feasibility of the developed method, we analyzed O-linked glycans of porcine submaxillary mucins separated by supported molecular matrix electrophoresis (SMME) which is previously developed to characterize mucins.
30952424	7	37	theme	O-linked	1129:1136	arg1	liberation					1145:1154	O-linked glycan liberation	1129:1154	O-linked glycan liberation	1129:1154	The method for O-linked glycan liberation and fluorescent labeling presented here was easy and rapid, and will be practically useful for O-linked glycan analyses.
30952424	1	38	theme	O-Linked	115:122	arg1	liberation					131:140	O-Linked glycan liberation	115:140	O-Linked glycan liberation from proteins through reductive beta-elimination and hydrazinolysis	115:208	O-Linked glycan liberation from proteins through reductive beta-elimination and hydrazinolysis is widely used, but have yet to satisfy the recent needs for glycan analysis in glycan biomarker research and microheterogeneity evaluation of biopharmaceutical glycosylation.
30952424	7	39	theme	glycan	1138:1143	arg1	liberation					1145:1154	O-linked glycan liberation	1129:1154	O-linked glycan liberation	1129:1154	The method for O-linked glycan liberation and fluorescent labeling presented here was easy and rapid, and will be practically useful for O-linked glycan analyses.
30952424	1	40	used	used	220:223	arg2	liberation					131:140	O-Linked glycan liberation	115:140	O-Linked glycan liberation from proteins through reductive beta-elimination and hydrazinolysis	115:208	O-Linked glycan liberation from proteins through reductive beta-elimination and hydrazinolysis is widely used, but have yet to satisfy the recent needs for glycan analysis in glycan biomarker research and microheterogeneity evaluation of biopharmaceutical glycosylation.
30952424	1	41	theme	glycan	124:129	arg1	liberation					131:140	O-Linked glycan liberation	115:140	O-Linked glycan liberation from proteins through reductive beta-elimination and hydrazinolysis	115:208	O-Linked glycan liberation from proteins through reductive beta-elimination and hydrazinolysis is widely used, but have yet to satisfy the recent needs for glycan analysis in glycan biomarker research and microheterogeneity evaluation of biopharmaceutical glycosylation.
30952424	6	42	theme	mucins	990:995	arg1	glycans					958:964	O-linked glycans	949:964	O-linked glycans of porcine submaxillary mucins separated by supported molecular matrix electrophoresis (SMME) which is previously developed to characterize mucins	949:1111	To demonstrate the feasibility of the developed method, we analyzed O-linked glycans of porcine submaxillary mucins separated by supported molecular matrix electrophoresis (SMME) which is previously developed to characterize mucins.
30952424	3	43	theme	degraded	647:654	arg1	products					656:663	less degraded products	642:663	less degraded products	642:663	The developed method afforded comparable results to those of hydrazinolysis, but with less degraded products.
30952424	1	44	dep	recent	254:259	arg1	needs					261:265	needs	261:265	needs	261:265	O-Linked glycan liberation from proteins through reductive beta-elimination and hydrazinolysis is widely used, but have yet to satisfy the recent needs for glycan analysis in glycan biomarker research and microheterogeneity evaluation of biopharmaceutical glycosylation.
30952424	7	45	link	O-linked	1251:1258	arg1	analyses					1267:1274	O-linked glycan analyses	1251:1274	O-linked glycan analyses	1251:1274	The method for O-linked glycan liberation and fluorescent labeling presented here was easy and rapid, and will be practically useful for O-linked glycan analyses.
30952424	5	46	theme	optimized	789:797	arg1	method					799:804	The optimized method	785:804	The optimized method	785:804	The optimized method also released glycans containing NeuGc without degradation or deacylation.
30952424	6	47	theme	submaxillary	977:988	arg1	mucins					990:995	porcine submaxillary mucins	969:995	porcine submaxillary mucins separated by supported molecular matrix electrophoresis (SMME) which is previously developed to characterize mucins	969:1111	To demonstrate the feasibility of the developed method, we analyzed O-linked glycans of porcine submaxillary mucins separated by supported molecular matrix electrophoresis (SMME) which is previously developed to characterize mucins.
30952424	7	48	link	O-linked	1129:1136	arg1	liberation					1145:1154	O-linked glycan liberation	1129:1154	O-linked glycan liberation	1129:1154	The method for O-linked glycan liberation and fluorescent labeling presented here was easy and rapid, and will be practically useful for O-linked glycan analyses.
30952424	4	49	theme	glycan	735:740	arg1	liberation					742:751	the optimized O-linked glycan liberation	712:751	the optimized O-linked glycan liberation	712:751	In addition, we examined the compatibility of the optimized O-linked glycan liberation with denaturant and detergents.
29311277	4	0	theme	undescribed	880:890	arg1	factors					911:917	previously undescribed septally localized factors	869:917	previously undescribed septally localized factors	869:917	Here, using Mycobacterium smegmatis, we identify and characterize homologs of the conserved cell division regulators FtsL and FtsB, and show that they appear to function similarly to their homologs in Escherichia coli We identify a number of previously undescribed septally localized factors which could be involved in cell wall regulation.
29311277	3	1	theme	growth	519:524	arg1	structure					495:503	cell wall structure	485:503	cell wall structure	485:503	Mycobacteria have both a cell wall structure and a mode of growth that are distinct from well-studied model organisms and use several different regulatory mechanisms.
29311277	3	1	theme	growth	519:524	arg1	mode					511:514	a mode	509:514	a mode of growth	509:524	Mycobacteria have both a cell wall structure and a mode of growth that are distinct from well-studied model organisms and use several different regulatory mechanisms.
29311277	3	1	theme	growth	519:524	arg1	distinct					535:542	distinct	535:542	distinct	535:542	Mycobacteria have both a cell wall structure and a mode of growth that are distinct from well-studied model organisms and use several different regulatory mechanisms.
29311277	6	2	theme	cell	1443:1446	arg1	lysis					1448:1452	cell lysis	1443:1452	cell lysis	1443:1452	We propose that SepIVA is a regulator of cell wall precursor enzymes that contribute to construction of the septal cross-wall, similar to the putative elongation function of the other mycobacterial DivIVA homolog, Wag31.IMPORTANCE The enzymes that build bacterial cell walls are essential for cell survival but can cause cell lysis if misregulated; thus, their regulators are also essential.
29311277	4	3	theme	localized	901:909	arg1	factors					911:917	previously undescribed septally localized factors	869:917	previously undescribed septally localized factors	869:917	Here, using Mycobacterium smegmatis, we identify and characterize homologs of the conserved cell division regulators FtsL and FtsB, and show that they appear to function similarly to their homologs in Escherichia coli We identify a number of previously undescribed septally localized factors which could be involved in cell wall regulation.
29311277	6	4	theme	cell	1163:1166	arg1	enzymes					1183:1189	cell wall precursor enzymes	1163:1189	cell wall precursor enzymes that contribute to construction of the septal cross-wall, similar to the putative elongation function of the other mycobacterial DivIVA homolog, Wag31.IMPORTANCE The enzymes that build bacterial cell walls are essential for cell survival but can cause cell lysis if misregulated	1163:1468	We propose that SepIVA is a regulator of cell wall precursor enzymes that contribute to construction of the septal cross-wall, similar to the putative elongation function of the other mycobacterial DivIVA homolog, Wag31.IMPORTANCE The enzymes that build bacterial cell walls are essential for cell survival but can cause cell lysis if misregulated; thus, their regulators are also essential.
29311277	9	5	theme	mycobacterial	1840:1852	arg1	wall					1859:1862	the mycobacterial cell wall	1836:1862	the mycobacterial cell wall	1836:1862	In this work, we identify and characterize some of the proteins that regulate the mycobacterial cell wall.
29311277	3	6	theme	model	562:566	arg1	organisms					568:576	well-studied model organisms	549:576	well-studied model organisms	549:576	Mycobacteria have both a cell wall structure and a mode of growth that are distinct from well-studied model organisms and use several different regulatory mechanisms.
29311277	8	7	theme	cell	1656:1659	arg1	wall					1661:1664	a cell wall	1654:1664	a cell wall whose composition and construction vary greatly from those of well-studied model organisms	1654:1755	The mycobacteria are a genus that have a cell wall whose composition and construction vary greatly from those of well-studied model organisms.
29311277	1	8	theme	existing	291:298	arg1	wall					305:308	the existing cell wall	287:308	the existing cell wall	287:308	Septation in bacteria requires coordinated regulation of cell wall biosynthesis and hydrolysis enzymes so that new septal cross-wall can be appropriately constructed without compromising the integrity of the existing cell wall.
29311277	10	9	theme	critical	2054:2061	arg1	functions					2074:2082	critical regulatory functions	2054:2082	critical regulatory functions that may be unique to the actinomycetes	2054:2122	We find that some of these regulators appear to be functionally conserved with their structural homologs in evolutionarily distant species such as Escherichia coli, but other proteins have critical regulatory functions that may be unique to the actinomycetes.
29311277	3	10	theme	several	586:592	arg1	mechanisms					615:624	several different regulatory mechanisms	586:624	several different regulatory mechanisms	586:624	Mycobacteria have both a cell wall structure and a mode of growth that are distinct from well-studied model organisms and use several different regulatory mechanisms.
29311277	1	11	theme	septal	198:203	arg1	cross-wall					205:214	new septal cross-wall	194:214	new septal cross-wall	194:214	Septation in bacteria requires coordinated regulation of cell wall biosynthesis and hydrolysis enzymes so that new septal cross-wall can be appropriately constructed without compromising the integrity of the existing cell wall.
29311277	6	12	theme	DivIVA	1320:1325	arg1	homolog					1327:1333	the other mycobacterial DivIVA homolog	1296:1333	the other mycobacterial DivIVA homolog	1296:1333	We propose that SepIVA is a regulator of cell wall precursor enzymes that contribute to construction of the septal cross-wall, similar to the putative elongation function of the other mycobacterial DivIVA homolog, Wag31.IMPORTANCE The enzymes that build bacterial cell walls are essential for cell survival but can cause cell lysis if misregulated; thus, their regulators are also essential.
29311277	4	13	theme	wall	951:954	arg1	regulation					956:965	cell wall regulation	946:965	cell wall regulation	946:965	Here, using Mycobacterium smegmatis, we identify and characterize homologs of the conserved cell division regulators FtsL and FtsB, and show that they appear to function similarly to their homologs in Escherichia coli We identify a number of previously undescribed septally localized factors which could be involved in cell wall regulation.
29311277	6	14	theme	other	1300:1304	arg1	homolog					1327:1333	the other mycobacterial DivIVA homolog	1296:1333	the other mycobacterial DivIVA homolog	1296:1333	We propose that SepIVA is a regulator of cell wall precursor enzymes that contribute to construction of the septal cross-wall, similar to the putative elongation function of the other mycobacterial DivIVA homolog, Wag31.IMPORTANCE The enzymes that build bacterial cell walls are essential for cell survival but can cause cell lysis if misregulated; thus, their regulators are also essential.
29311277	5	15	theme	mycobacterial	1027:1039	arg1	septation					1041:1049	mycobacterial septation	1027:1049	mycobacterial septation	1027:1049	One of these, SepIVA, has a DivIVA domain, is required for mycobacterial septation, and is localized to the septum and the intracellular membrane domain.
29311277	3	16	theme	regulatory	604:613	arg1	mechanisms					615:624	several different regulatory mechanisms	586:624	several different regulatory mechanisms	586:624	Mycobacteria have both a cell wall structure and a mode of growth that are distinct from well-studied model organisms and use several different regulatory mechanisms.
29311277	6	17	theme	elongation	1273:1282	arg1	function					1284:1291	the putative elongation function	1260:1291	the putative elongation function of the other mycobacterial DivIVA homolog	1260:1333	We propose that SepIVA is a regulator of cell wall precursor enzymes that contribute to construction of the septal cross-wall, similar to the putative elongation function of the other mycobacterial DivIVA homolog, Wag31.IMPORTANCE The enzymes that build bacterial cell walls are essential for cell survival but can cause cell lysis if misregulated; thus, their regulators are also essential.
29311277	8	18	theme	model	1741:1745	arg1	organisms					1747:1755	well-studied model organisms	1728:1755	well-studied model organisms	1728:1755	The mycobacteria are a genus that have a cell wall whose composition and construction vary greatly from those of well-studied model organisms.
29311277	1	19	theme	wall	145:148	arg1	biosynthesis					150:161	cell wall biosynthesis	140:161	cell wall biosynthesis	140:161	Septation in bacteria requires coordinated regulation of cell wall biosynthesis and hydrolysis enzymes so that new septal cross-wall can be appropriately constructed without compromising the integrity of the existing cell wall.
29311277	5	20	contain	has	990:992	arg1	One					968:970	One	968:970	One	968:970	One of these, SepIVA, has a DivIVA domain, is required for mycobacterial septation, and is localized to the septum and the intracellular membrane domain.
29311277	5	20	contain	has	990:992	arg1	SepIVA					982:987	SepIVA	982:987	SepIVA	982:987	One of these, SepIVA, has a DivIVA domain, is required for mycobacterial septation, and is localized to the septum and the intracellular membrane domain.
29311277	5	20	contain	has	990:992	arg2	domain					1003:1008	a DivIVA domain	994:1008	a DivIVA domain	994:1008	One of these, SepIVA, has a DivIVA domain, is required for mycobacterial septation, and is localized to the septum and the intracellular membrane domain.
29311277	5	20	contain	has	990:992	arg1	these					975:979	these	975:979	these	975:979	One of these, SepIVA, has a DivIVA domain, is required for mycobacterial septation, and is localized to the septum and the intracellular membrane domain.
29311277	6	21	theme	precursor	1173:1181	arg1	enzymes					1183:1189	cell wall precursor enzymes	1163:1189	cell wall precursor enzymes that contribute to construction of the septal cross-wall, similar to the putative elongation function of the other mycobacterial DivIVA homolog, Wag31.IMPORTANCE The enzymes that build bacterial cell walls are essential for cell survival but can cause cell lysis if misregulated	1163:1468	We propose that SepIVA is a regulator of cell wall precursor enzymes that contribute to construction of the septal cross-wall, similar to the putative elongation function of the other mycobacterial DivIVA homolog, Wag31.IMPORTANCE The enzymes that build bacterial cell walls are essential for cell survival but can cause cell lysis if misregulated; thus, their regulators are also essential.
29311277	3	22	theme	cell	485:488	arg1	structure					495:503	cell wall structure	485:503	cell wall structure	485:503	Mycobacteria have both a cell wall structure and a mode of growth that are distinct from well-studied model organisms and use several different regulatory mechanisms.
29311277	4	23	theme	conserved	709:717	arg1	FtsL					744:747	FtsL	744:747	FtsL	744:747	Here, using Mycobacterium smegmatis, we identify and characterize homologs of the conserved cell division regulators FtsL and FtsB, and show that they appear to function similarly to their homologs in Escherichia coli We identify a number of previously undescribed septally localized factors which could be involved in cell wall regulation.
29311277	4	23	theme	conserved	709:717	arg1	regulators					733:742	the conserved cell division regulators FtsL and FtsB	705:756	the conserved cell division regulators FtsL and FtsB	705:756	Here, using Mycobacterium smegmatis, we identify and characterize homologs of the conserved cell division regulators FtsL and FtsB, and show that they appear to function similarly to their homologs in Escherichia coli We identify a number of previously undescribed septally localized factors which could be involved in cell wall regulation.
29311277	4	23	theme	conserved	709:717	arg1	FtsB					753:756	FtsB	753:756	FtsB	753:756	Here, using Mycobacterium smegmatis, we identify and characterize homologs of the conserved cell division regulators FtsL and FtsB, and show that they appear to function similarly to their homologs in Escherichia coli We identify a number of previously undescribed septally localized factors which could be involved in cell wall regulation.
29311277	10	24	theme	structural	1950:1959	arg1	homologs					1961:1968	their structural homologs	1944:1968	their structural homologs in evolutionarily distant species such as Escherichia coli	1944:2027	We find that some of these regulators appear to be functionally conserved with their structural homologs in evolutionarily distant species such as Escherichia coli, but other proteins have critical regulatory functions that may be unique to the actinomycetes.
29311277	6	25	theme	bacterial	1376:1384	arg1	walls					1391:1395	build bacterial cell walls	1370:1395	build bacterial cell walls	1370:1395	We propose that SepIVA is a regulator of cell wall precursor enzymes that contribute to construction of the septal cross-wall, similar to the putative elongation function of the other mycobacterial DivIVA homolog, Wag31.IMPORTANCE The enzymes that build bacterial cell walls are essential for cell survival but can cause cell lysis if misregulated; thus, their regulators are also essential.
29311277	5	26	theme	membrane	1105:1112	arg1	domain					1114:1119	the intracellular membrane domain	1087:1119	the intracellular membrane domain	1087:1119	One of these, SepIVA, has a DivIVA domain, is required for mycobacterial septation, and is localized to the septum and the intracellular membrane domain.
29311277	6	27	theme	cell	1415:1418	arg1	survival					1420:1427	cell survival	1415:1427	cell survival	1415:1427	We propose that SepIVA is a regulator of cell wall precursor enzymes that contribute to construction of the septal cross-wall, similar to the putative elongation function of the other mycobacterial DivIVA homolog, Wag31.IMPORTANCE The enzymes that build bacterial cell walls are essential for cell survival but can cause cell lysis if misregulated; thus, their regulators are also essential.
29311277	4	28	theme	division	724:731	arg1	FtsL					744:747	FtsL	744:747	FtsL	744:747	Here, using Mycobacterium smegmatis, we identify and characterize homologs of the conserved cell division regulators FtsL and FtsB, and show that they appear to function similarly to their homologs in Escherichia coli We identify a number of previously undescribed septally localized factors which could be involved in cell wall regulation.
29311277	4	28	theme	division	724:731	arg1	regulators					733:742	the conserved cell division regulators FtsL and FtsB	705:756	the conserved cell division regulators FtsL and FtsB	705:756	Here, using Mycobacterium smegmatis, we identify and characterize homologs of the conserved cell division regulators FtsL and FtsB, and show that they appear to function similarly to their homologs in Escherichia coli We identify a number of previously undescribed septally localized factors which could be involved in cell wall regulation.
29311277	4	28	theme	division	724:731	arg1	FtsB					753:756	FtsB	753:756	FtsB	753:756	Here, using Mycobacterium smegmatis, we identify and characterize homologs of the conserved cell division regulators FtsL and FtsB, and show that they appear to function similarly to their homologs in Escherichia coli We identify a number of previously undescribed septally localized factors which could be involved in cell wall regulation.
29311277	1	29	theme	enzymes	178:184	arg1	regulation					126:135	coordinated regulation	114:135	coordinated regulation of cell wall biosynthesis and hydrolysis enzymes so that new septal cross-wall can be appropriately constructed without compromising the integrity of the existing cell wall	114:308	Septation in bacteria requires coordinated regulation of cell wall biosynthesis and hydrolysis enzymes so that new septal cross-wall can be appropriately constructed without compromising the integrity of the existing cell wall.
29311277	0	30	from	Characterization	0:15	arg1	smegmatis					72:80	Mycobacterium smegmatis	58:80	Mycobacterium smegmatis	58:80	Characterization of Conserved and Novel Septal Factors in Mycobacterium smegmatis.
29311277	1	31	theme	coordinated	114:124	arg1	regulation					126:135	coordinated regulation	114:135	coordinated regulation of cell wall biosynthesis and hydrolysis enzymes so that new septal cross-wall can be appropriately constructed without compromising the integrity of the existing cell wall	114:308	Septation in bacteria requires coordinated regulation of cell wall biosynthesis and hydrolysis enzymes so that new septal cross-wall can be appropriately constructed without compromising the integrity of the existing cell wall.
29311277	10	32	theme	distant	1988:1994	arg1	coli					2024:2027	Escherichia coli	2012:2027	Escherichia coli	2012:2027	We find that some of these regulators appear to be functionally conserved with their structural homologs in evolutionarily distant species such as Escherichia coli, but other proteins have critical regulatory functions that may be unique to the actinomycetes.
29311277	10	32	theme	distant	1988:1994	arg1	species					1996:2002	evolutionarily distant species	1973:2002	evolutionarily distant species such as Escherichia coli	1973:2027	We find that some of these regulators appear to be functionally conserved with their structural homologs in evolutionarily distant species such as Escherichia coli, but other proteins have critical regulatory functions that may be unique to the actinomycetes.
29311277	6	33	theme	The	1353:1355	arg1	enzymes					1357:1363	the septal cross-wall, similar to the putative elongation function of the other mycobacterial DivIVA homolog, Wag31.IMPORTANCE The enzymes	1226:1363	the septal cross-wall, similar to the putative elongation function of the other mycobacterial DivIVA homolog, Wag31.IMPORTANCE The enzymes	1226:1363	We propose that SepIVA is a regulator of cell wall precursor enzymes that contribute to construction of the septal cross-wall, similar to the putative elongation function of the other mycobacterial DivIVA homolog, Wag31.IMPORTANCE The enzymes that build bacterial cell walls are essential for cell survival but can cause cell lysis if misregulated; thus, their regulators are also essential.
29311277	6	34	dep	The	1353:1355	arg1	Wag31.IMPORTANCE					1336:1351	Wag31.IMPORTANCE	1336:1351	Wag31.IMPORTANCE	1336:1351	We propose that SepIVA is a regulator of cell wall precursor enzymes that contribute to construction of the septal cross-wall, similar to the putative elongation function of the other mycobacterial DivIVA homolog, Wag31.IMPORTANCE The enzymes that build bacterial cell walls are essential for cell survival but can cause cell lysis if misregulated; thus, their regulators are also essential.
29311277	1	35	theme	cell	300:303	arg1	wall					305:308	the existing cell wall	287:308	the existing cell wall	287:308	Septation in bacteria requires coordinated regulation of cell wall biosynthesis and hydrolysis enzymes so that new septal cross-wall can be appropriately constructed without compromising the integrity of the existing cell wall.
29311277	2	36	theme	division	440:447	arg1	processes					449:457	division processes	440:457	division processes	440:457	Bacteria with different modes of growth and different types of cell wall require different regulators to mediate cell growth and division processes.
29311277	0	37	theme	Conserved	20:28	arg1	Factors					47:53	Conserved and Novel Septal Factors	20:53	Conserved and Novel Septal Factors	20:53	Characterization of Conserved and Novel Septal Factors in Mycobacterium smegmatis.
29311277	4	38	dep	regulators	733:742	arg1	FtsL					744:747	FtsL	744:747	FtsL	744:747	Here, using Mycobacterium smegmatis, we identify and characterize homologs of the conserved cell division regulators FtsL and FtsB, and show that they appear to function similarly to their homologs in Escherichia coli We identify a number of previously undescribed septally localized factors which could be involved in cell wall regulation.
29311277	4	38	dep	regulators	733:742	arg1	regulators					733:742	the conserved cell division regulators FtsL and FtsB	705:756	the conserved cell division regulators FtsL and FtsB	705:756	Here, using Mycobacterium smegmatis, we identify and characterize homologs of the conserved cell division regulators FtsL and FtsB, and show that they appear to function similarly to their homologs in Escherichia coli We identify a number of previously undescribed septally localized factors which could be involved in cell wall regulation.
29311277	4	38	dep	regulators	733:742	arg1	FtsB					753:756	FtsB	753:756	FtsB	753:756	Here, using Mycobacterium smegmatis, we identify and characterize homologs of the conserved cell division regulators FtsL and FtsB, and show that they appear to function similarly to their homologs in Escherichia coli We identify a number of previously undescribed septally localized factors which could be involved in cell wall regulation.
29311277	0	39	theme	Novel	34:38	arg1	Factors					47:53	Conserved and Novel Septal Factors	20:53	Conserved and Novel Septal Factors	20:53	Characterization of Conserved and Novel Septal Factors in Mycobacterium smegmatis.
29311277	10	40	from	homologs	1961:1968	arg1	coli					2024:2027	Escherichia coli	2012:2027	Escherichia coli	2012:2027	We find that some of these regulators appear to be functionally conserved with their structural homologs in evolutionarily distant species such as Escherichia coli, but other proteins have critical regulatory functions that may be unique to the actinomycetes.
29311277	10	40	from	homologs	1961:1968	arg1	species					1996:2002	evolutionarily distant species	1973:2002	evolutionarily distant species such as Escherichia coli	1973:2027	We find that some of these regulators appear to be functionally conserved with their structural homologs in evolutionarily distant species such as Escherichia coli, but other proteins have critical regulatory functions that may be unique to the actinomycetes.
29311277	2	41	with	types	365:369	arg1	modes					335:339	different modes	325:339	different modes of growth	325:349	Bacteria with different modes of growth and different types of cell wall require different regulators to mediate cell growth and division processes.
29311277	2	42	with	Bacteria	311:318	arg1	modes					335:339	different modes	325:339	different modes of growth	325:349	Bacteria with different modes of growth and different types of cell wall require different regulators to mediate cell growth and division processes.
29311277	0	43	theme	Mycobacterium	58:70	arg1	smegmatis					72:80	Mycobacterium smegmatis	58:80	Mycobacterium smegmatis	58:80	Characterization of Conserved and Novel Septal Factors in Mycobacterium smegmatis.
29311277	0	44	theme	Factors	47:53	arg1	Characterization					0:15	Characterization	0:15	Characterization of Conserved and Novel Septal Factors in Mycobacterium smegmatis.	0:81	Characterization of Conserved and Novel Septal Factors in Mycobacterium smegmatis.
29311277	10	45	contain	have	2049:2052	arg1	proteins					2040:2047	other proteins	2034:2047	other proteins	2034:2047	We find that some of these regulators appear to be functionally conserved with their structural homologs in evolutionarily distant species such as Escherichia coli, but other proteins have critical regulatory functions that may be unique to the actinomycetes.
29311277	10	45	contain	have	2049:2052	arg2	functions					2074:2082	critical regulatory functions	2054:2082	critical regulatory functions that may be unique to the actinomycetes	2054:2122	We find that some of these regulators appear to be functionally conserved with their structural homologs in evolutionarily distant species such as Escherichia coli, but other proteins have critical regulatory functions that may be unique to the actinomycetes.
29311277	4	46	from	homologs	816:823	arg1	coli					840:843	Escherichia coli	828:843	Escherichia coli	828:843	Here, using Mycobacterium smegmatis, we identify and characterize homologs of the conserved cell division regulators FtsL and FtsB, and show that they appear to function similarly to their homologs in Escherichia coli We identify a number of previously undescribed septally localized factors which could be involved in cell wall regulation.
29311277	6	47	theme	putative	1264:1271	arg1	function					1284:1291	the putative elongation function	1260:1291	the putative elongation function of the other mycobacterial DivIVA homolog	1260:1333	We propose that SepIVA is a regulator of cell wall precursor enzymes that contribute to construction of the septal cross-wall, similar to the putative elongation function of the other mycobacterial DivIVA homolog, Wag31.IMPORTANCE The enzymes that build bacterial cell walls are essential for cell survival but can cause cell lysis if misregulated; thus, their regulators are also essential.
29311277	2	48	theme	cell	374:377	arg1	wall					379:382	cell wall	374:382	cell wall	374:382	Bacteria with different modes of growth and different types of cell wall require different regulators to mediate cell growth and division processes.
29311277	1	49	from	Septation	83:91	arg1	bacteria					96:103	bacteria	96:103	bacteria	96:103	Septation in bacteria requires coordinated regulation of cell wall biosynthesis and hydrolysis enzymes so that new septal cross-wall can be appropriately constructed without compromising the integrity of the existing cell wall.
29311277	10	50	theme	other	2034:2038	arg1	proteins					2040:2047	other proteins	2034:2047	other proteins	2034:2047	We find that some of these regulators appear to be functionally conserved with their structural homologs in evolutionarily distant species such as Escherichia coli, but other proteins have critical regulatory functions that may be unique to the actinomycetes.
29311277	3	51	theme	well-studied	549:560	arg1	organisms					568:576	well-studied model organisms	549:576	well-studied model organisms	549:576	Mycobacteria have both a cell wall structure and a mode of growth that are distinct from well-studied model organisms and use several different regulatory mechanisms.
29311277	9	52	theme	cell	1854:1857	arg1	wall					1859:1862	the mycobacterial cell wall	1836:1862	the mycobacterial cell wall	1836:1862	In this work, we identify and characterize some of the proteins that regulate the mycobacterial cell wall.
29311277	4	53	dep	homologs	816:823	arg1	identify					848:855	identify	848:855	identify a number of previously undescribed septally localized factors which could be involved in cell wall regulation	848:965	Here, using Mycobacterium smegmatis, we identify and characterize homologs of the conserved cell division regulators FtsL and FtsB, and show that they appear to function similarly to their homologs in Escherichia coli We identify a number of previously undescribed septally localized factors which could be involved in cell wall regulation.
29311277	10	54	theme	regulatory	2063:2072	arg1	functions					2074:2082	critical regulatory functions	2054:2082	critical regulatory functions that may be unique to the actinomycetes	2054:2122	We find that some of these regulators appear to be functionally conserved with their structural homologs in evolutionarily distant species such as Escherichia coli, but other proteins have critical regulatory functions that may be unique to the actinomycetes.
29311277	6	55	theme	septal	1230:1235	arg1	enzymes					1357:1363	the septal cross-wall, similar to the putative elongation function of the other mycobacterial DivIVA homolog, Wag31.IMPORTANCE The enzymes	1226:1363	the septal cross-wall, similar to the putative elongation function of the other mycobacterial DivIVA homolog, Wag31.IMPORTANCE The enzymes	1226:1363	We propose that SepIVA is a regulator of cell wall precursor enzymes that contribute to construction of the septal cross-wall, similar to the putative elongation function of the other mycobacterial DivIVA homolog, Wag31.IMPORTANCE The enzymes that build bacterial cell walls are essential for cell survival but can cause cell lysis if misregulated; thus, their regulators are also essential.
29311277	1	56	theme	new	194:196	arg1	cross-wall					205:214	new septal cross-wall	194:214	new septal cross-wall	194:214	Septation in bacteria requires coordinated regulation of cell wall biosynthesis and hydrolysis enzymes so that new septal cross-wall can be appropriately constructed without compromising the integrity of the existing cell wall.
29311277	7	57	theme	different	1599:1607	arg1	ways					1609:1612	different ways	1599:1612	different ways	1599:1612	The number and nature of these regulators is likely to vary in bacteria that grow in different ways.
29311277	6	58	theme	homolog	1327:1333	arg1	function					1284:1291	the putative elongation function	1260:1291	the putative elongation function of the other mycobacterial DivIVA homolog	1260:1333	We propose that SepIVA is a regulator of cell wall precursor enzymes that contribute to construction of the septal cross-wall, similar to the putative elongation function of the other mycobacterial DivIVA homolog, Wag31.IMPORTANCE The enzymes that build bacterial cell walls are essential for cell survival but can cause cell lysis if misregulated; thus, their regulators are also essential.
29311277	2	59	theme	different	325:333	arg1	modes					335:339	different modes	325:339	different modes of growth	325:349	Bacteria with different modes of growth and different types of cell wall require different regulators to mediate cell growth and division processes.
29311277	6	60	theme	mycobacterial	1306:1318	arg1	homolog					1327:1333	the other mycobacterial DivIVA homolog	1296:1333	the other mycobacterial DivIVA homolog	1296:1333	We propose that SepIVA is a regulator of cell wall precursor enzymes that contribute to construction of the septal cross-wall, similar to the putative elongation function of the other mycobacterial DivIVA homolog, Wag31.IMPORTANCE The enzymes that build bacterial cell walls are essential for cell survival but can cause cell lysis if misregulated; thus, their regulators are also essential.
29311277	8	61	contain	have	1649:1652	arg1	mycobacteria					1619:1630	The mycobacteria	1615:1630	The mycobacteria	1615:1630	The mycobacteria are a genus that have a cell wall whose composition and construction vary greatly from those of well-studied model organisms.
29311277	8	61	contain	have	1649:1652	arg1	genus					1638:1642	a genus	1636:1642	a genus that have a cell wall whose composition and construction vary greatly from those of well-studied model organisms	1636:1755	The mycobacteria are a genus that have a cell wall whose composition and construction vary greatly from those of well-studied model organisms.
29311277	8	61	contain	have	1649:1652	arg2	wall					1661:1664	a cell wall	1654:1664	a cell wall whose composition and construction vary greatly from those of well-studied model organisms	1654:1755	The mycobacteria are a genus that have a cell wall whose composition and construction vary greatly from those of well-studied model organisms.
29311277	4	62	theme	cell	946:949	arg1	regulation					956:965	cell wall regulation	946:965	cell wall regulation	946:965	Here, using Mycobacterium smegmatis, we identify and characterize homologs of the conserved cell division regulators FtsL and FtsB, and show that they appear to function similarly to their homologs in Escherichia coli We identify a number of previously undescribed septally localized factors which could be involved in cell wall regulation.
29311277	3	63	theme	different	594:602	arg1	mechanisms					615:624	several different regulatory mechanisms	586:624	several different regulatory mechanisms	586:624	Mycobacteria have both a cell wall structure and a mode of growth that are distinct from well-studied model organisms and use several different regulatory mechanisms.
29311277	4	64	theme	factors	911:917	arg1	number					859:864	a number	857:864	a number of previously undescribed septally localized factors which could be involved in cell wall regulation	857:965	Here, using Mycobacterium smegmatis, we identify and characterize homologs of the conserved cell division regulators FtsL and FtsB, and show that they appear to function similarly to their homologs in Escherichia coli We identify a number of previously undescribed septally localized factors which could be involved in cell wall regulation.
29311277	3	65	dep	structure	495:503	arg1	a					483:483	a	483:483	a	483:483	Mycobacteria have both a cell wall structure and a mode of growth that are distinct from well-studied model organisms and use several different regulatory mechanisms.
29311277	4	66	theme	regulators	733:742	arg1	homologs					693:700	homologs	693:700	homologs of the conserved cell division regulators FtsL and FtsB	693:756	Here, using Mycobacterium smegmatis, we identify and characterize homologs of the conserved cell division regulators FtsL and FtsB, and show that they appear to function similarly to their homologs in Escherichia coli We identify a number of previously undescribed septally localized factors which could be involved in cell wall regulation.
29311277	3	67	from	organisms	568:576	arg1	distinct					535:542	distinct	535:542	distinct	535:542	Mycobacteria have both a cell wall structure and a mode of growth that are distinct from well-studied model organisms and use several different regulatory mechanisms.
29311277	3	67	from	organisms	568:576	arg1	mode					511:514	a mode	509:514	a mode of growth	509:524	Mycobacteria have both a cell wall structure and a mode of growth that are distinct from well-studied model organisms and use several different regulatory mechanisms.
29311277	1	68	theme	cell	140:143	arg1	biosynthesis					150:161	cell wall biosynthesis	140:161	cell wall biosynthesis	140:161	Septation in bacteria requires coordinated regulation of cell wall biosynthesis and hydrolysis enzymes so that new septal cross-wall can be appropriately constructed without compromising the integrity of the existing cell wall.
29311277	3	69	contain	have	473:476	arg1	Mycobacteria					460:471	Mycobacteria	460:471	Mycobacteria	460:471	Mycobacteria have both a cell wall structure and a mode of growth that are distinct from well-studied model organisms and use several different regulatory mechanisms.
29311277	3	69	contain	have	473:476	arg2	structure					495:503	cell wall structure	485:503	cell wall structure	485:503	Mycobacteria have both a cell wall structure and a mode of growth that are distinct from well-studied model organisms and use several different regulatory mechanisms.
29311277	3	69	contain	have	473:476	arg2	mode					511:514	a mode	509:514	a mode of growth	509:524	Mycobacteria have both a cell wall structure and a mode of growth that are distinct from well-studied model organisms and use several different regulatory mechanisms.
29311277	3	69	contain	have	473:476	arg2	distinct					535:542	distinct	535:542	distinct	535:542	Mycobacteria have both a cell wall structure and a mode of growth that are distinct from well-studied model organisms and use several different regulatory mechanisms.
29311277	6	70	theme	enzymes	1183:1189	arg1	SepIVA					1138:1143	SepIVA	1138:1143	SepIVA	1138:1143	We propose that SepIVA is a regulator of cell wall precursor enzymes that contribute to construction of the septal cross-wall, similar to the putative elongation function of the other mycobacterial DivIVA homolog, Wag31.IMPORTANCE The enzymes that build bacterial cell walls are essential for cell survival but can cause cell lysis if misregulated; thus, their regulators are also essential.
29311277	6	70	theme	enzymes	1183:1189	arg1	regulator					1150:1158	a regulator	1148:1158	a regulator of cell wall precursor enzymes that contribute to construction of the septal cross-wall, similar to the putative elongation function of the other mycobacterial DivIVA homolog, Wag31.IMPORTANCE The enzymes that build bacterial cell walls are essential for cell survival but can cause cell lysis if misregulated	1148:1468	We propose that SepIVA is a regulator of cell wall precursor enzymes that contribute to construction of the septal cross-wall, similar to the putative elongation function of the other mycobacterial DivIVA homolog, Wag31.IMPORTANCE The enzymes that build bacterial cell walls are essential for cell survival but can cause cell lysis if misregulated; thus, their regulators are also essential.
29311277	6	71	theme	cell	1386:1389	arg1	walls					1391:1395	build bacterial cell walls	1370:1395	build bacterial cell walls	1370:1395	We propose that SepIVA is a regulator of cell wall precursor enzymes that contribute to construction of the septal cross-wall, similar to the putative elongation function of the other mycobacterial DivIVA homolog, Wag31.IMPORTANCE The enzymes that build bacterial cell walls are essential for cell survival but can cause cell lysis if misregulated; thus, their regulators are also essential.
29311277	8	72	theme	well-studied	1728:1739	arg1	organisms					1747:1755	well-studied model organisms	1728:1755	well-studied model organisms	1728:1755	The mycobacteria are a genus that have a cell wall whose composition and construction vary greatly from those of well-studied model organisms.
29311277	1	73	theme	biosynthesis	150:161	arg1	enzymes					178:184	cell wall biosynthesis and hydrolysis enzymes	140:184	cell wall biosynthesis and hydrolysis enzymes so that new septal cross-wall can be appropriately constructed without compromising the integrity of the existing cell wall	140:308	Septation in bacteria requires coordinated regulation of cell wall biosynthesis and hydrolysis enzymes so that new septal cross-wall can be appropriately constructed without compromising the integrity of the existing cell wall.
29311277	7	74	theme	regulators	1545:1554	arg1	nature					1529:1534	nature	1529:1534	nature	1529:1534	The number and nature of these regulators is likely to vary in bacteria that grow in different ways.
29311277	7	74	theme	regulators	1545:1554	arg1	number					1518:1523	number	1518:1523	number	1518:1523	The number and nature of these regulators is likely to vary in bacteria that grow in different ways.
29311277	6	75	theme	wall	1168:1171	arg1	enzymes					1183:1189	cell wall precursor enzymes	1163:1189	cell wall precursor enzymes that contribute to construction of the septal cross-wall, similar to the putative elongation function of the other mycobacterial DivIVA homolog, Wag31.IMPORTANCE The enzymes that build bacterial cell walls are essential for cell survival but can cause cell lysis if misregulated	1163:1468	We propose that SepIVA is a regulator of cell wall precursor enzymes that contribute to construction of the septal cross-wall, similar to the putative elongation function of the other mycobacterial DivIVA homolog, Wag31.IMPORTANCE The enzymes that build bacterial cell walls are essential for cell survival but can cause cell lysis if misregulated; thus, their regulators are also essential.
29311277	6	76	theme	build	1370:1374	arg1	walls					1391:1395	build bacterial cell walls	1370:1395	build bacterial cell walls	1370:1395	We propose that SepIVA is a regulator of cell wall precursor enzymes that contribute to construction of the septal cross-wall, similar to the putative elongation function of the other mycobacterial DivIVA homolog, Wag31.IMPORTANCE The enzymes that build bacterial cell walls are essential for cell survival but can cause cell lysis if misregulated; thus, their regulators are also essential.
29311277	1	77	theme	hydrolysis	167:176	arg1	enzymes					178:184	cell wall biosynthesis and hydrolysis enzymes	140:184	cell wall biosynthesis and hydrolysis enzymes so that new septal cross-wall can be appropriately constructed without compromising the integrity of the existing cell wall	140:308	Septation in bacteria requires coordinated regulation of cell wall biosynthesis and hydrolysis enzymes so that new septal cross-wall can be appropriately constructed without compromising the integrity of the existing cell wall.
29311277	4	78	theme	cell	719:722	arg1	FtsL					744:747	FtsL	744:747	FtsL	744:747	Here, using Mycobacterium smegmatis, we identify and characterize homologs of the conserved cell division regulators FtsL and FtsB, and show that they appear to function similarly to their homologs in Escherichia coli We identify a number of previously undescribed septally localized factors which could be involved in cell wall regulation.
29311277	4	78	theme	cell	719:722	arg1	regulators					733:742	the conserved cell division regulators FtsL and FtsB	705:756	the conserved cell division regulators FtsL and FtsB	705:756	Here, using Mycobacterium smegmatis, we identify and characterize homologs of the conserved cell division regulators FtsL and FtsB, and show that they appear to function similarly to their homologs in Escherichia coli We identify a number of previously undescribed septally localized factors which could be involved in cell wall regulation.
29311277	4	78	theme	cell	719:722	arg1	FtsB					753:756	FtsB	753:756	FtsB	753:756	Here, using Mycobacterium smegmatis, we identify and characterize homologs of the conserved cell division regulators FtsL and FtsB, and show that they appear to function similarly to their homologs in Escherichia coli We identify a number of previously undescribed septally localized factors which could be involved in cell wall regulation.
29311277	6	79	theme	enzymes	1357:1363	arg1	construction					1210:1221	construction	1210:1221	construction of the septal cross-wall, similar to the putative elongation function of the other mycobacterial DivIVA homolog, Wag31.IMPORTANCE The enzymes	1210:1363	We propose that SepIVA is a regulator of cell wall precursor enzymes that contribute to construction of the septal cross-wall, similar to the putative elongation function of the other mycobacterial DivIVA homolog, Wag31.IMPORTANCE The enzymes that build bacterial cell walls are essential for cell survival but can cause cell lysis if misregulated; thus, their regulators are also essential.
29311277	5	80	theme	these	975:979	arg1	One					968:970	One	968:970	One	968:970	One of these, SepIVA, has a DivIVA domain, is required for mycobacterial septation, and is localized to the septum and the intracellular membrane domain.
29311277	5	80	theme	these	975:979	arg1	SepIVA					982:987	SepIVA	982:987	SepIVA	982:987	One of these, SepIVA, has a DivIVA domain, is required for mycobacterial septation, and is localized to the septum and the intracellular membrane domain.
29311277	5	80	theme	these	975:979	arg1	these					975:979	these	975:979	these	975:979	One of these, SepIVA, has a DivIVA domain, is required for mycobacterial septation, and is localized to the septum and the intracellular membrane domain.
29311277	5	81	theme	intracellular	1091:1103	arg1	domain					1114:1119	the intracellular membrane domain	1087:1119	the intracellular membrane domain	1087:1119	One of these, SepIVA, has a DivIVA domain, is required for mycobacterial septation, and is localized to the septum and the intracellular membrane domain.
29311277	2	82	theme	different	392:400	arg1	regulators					402:411	different regulators	392:411	different regulators	392:411	Bacteria with different modes of growth and different types of cell wall require different regulators to mediate cell growth and division processes.
29311277	1	83	theme	wall	305:308	arg1	integrity					274:282	the integrity	270:282	the integrity of the existing cell wall	270:308	Septation in bacteria requires coordinated regulation of cell wall biosynthesis and hydrolysis enzymes so that new septal cross-wall can be appropriately constructed without compromising the integrity of the existing cell wall.
29311277	3	84	theme	wall	490:493	arg1	structure					495:503	cell wall structure	485:503	cell wall structure	485:503	Mycobacteria have both a cell wall structure and a mode of growth that are distinct from well-studied model organisms and use several different regulatory mechanisms.
29311277	0	85	theme	Septal	40:45	arg1	Factors					47:53	Conserved and Novel Septal Factors	20:53	Conserved and Novel Septal Factors	20:53	Characterization of Conserved and Novel Septal Factors in Mycobacterium smegmatis.
29311277	2	86	theme	cell	424:427	arg1	growth					429:434	cell growth	424:434	cell growth	424:434	Bacteria with different modes of growth and different types of cell wall require different regulators to mediate cell growth and division processes.
29311277	2	87	theme	different	355:363	arg1	types					365:369	different types	355:369	different types of cell wall	355:382	Bacteria with different modes of growth and different types of cell wall require different regulators to mediate cell growth and division processes.
29311277	2	88	theme	growth	344:349	arg1	modes					335:339	different modes	325:339	different modes of growth	325:349	Bacteria with different modes of growth and different types of cell wall require different regulators to mediate cell growth and division processes.
29311277	5	89	theme	DivIVA	996:1001	arg1	domain					1003:1008	a DivIVA domain	994:1008	a DivIVA domain	994:1008	One of these, SepIVA, has a DivIVA domain, is required for mycobacterial septation, and is localized to the septum and the intracellular membrane domain.
29311277	4	90	theme	Mycobacterium	639:651	arg1	smegmatis					653:661	Mycobacterium smegmatis	639:661	Mycobacterium smegmatis	639:661	Here, using Mycobacterium smegmatis, we identify and characterize homologs of the conserved cell division regulators FtsL and FtsB, and show that they appear to function similarly to their homologs in Escherichia coli We identify a number of previously undescribed septally localized factors which could be involved in cell wall regulation.
29311277	2	91	theme	wall	379:382	arg1	Bacteria					311:318	Bacteria	311:318	Bacteria with different modes of growth	311:349	Bacteria with different modes of growth and different types of cell wall require different regulators to mediate cell growth and division processes.
29311277	2	91	theme	wall	379:382	arg1	types					365:369	different types	355:369	different types of cell wall	355:382	Bacteria with different modes of growth and different types of cell wall require different regulators to mediate cell growth and division processes.
29311277	7	92	dep	number	1518:1523	arg1	The					1514:1516	The	1514:1516	The	1514:1516	The number and nature of these regulators is likely to vary in bacteria that grow in different ways.
29877707	0	0	theme	Enhanced	80:87	arg1	Activity					103:110	Enhanced Antimicrobial Activity	80:110	Enhanced Antimicrobial Activity	80:110	Electrospun Chitosan/Poly(ethylene oxide)/Lauric Arginate Nanofibrous Film with Enhanced Antimicrobial Activity.
29877707	4	1	theme	polymer	888:894	arg1	molecules					896:904	the polymer molecules	884:904	the polymer molecules	884:904	The formation of electrostatic and hydrogen bonding interactions induced by the LAE addition changed the inter- and intramolecular interactions between PEO and chitosan and further affected the mobility of the polymer molecules, leading to the increased crystallinity and decreased melting point.
29877707	2	2	theme	acetic	329:334	arg1	solutions					344:352	acetic aqueous solutions	329:352	acetic aqueous solutions	329:352	The addition of LAE did not change the physical properties of chitosan/PEO in acetic aqueous solutions, but increased the fluorescent intensity of chitosan by electrostatic interactions, resulting in uniform and bead-free nanofibers with an average diameter of 150 nm.
29877707	7	3	theme	nanofibrous	1438:1448	arg1	films					1450:1454	the nanofibrous films	1434:1454	the nanofibrous films	1434:1454	The fluorescent staining experiments demonstrated that the antimicrobial mechanism of the nanofibrous films was cell membrane damage.
29877707	2	4	theme	bead-free	463:471	arg1	nanofibers					473:482	uniform and bead-free nanofibers	451:482	uniform and bead-free nanofibers with an average diameter of 150 nm	451:517	The addition of LAE did not change the physical properties of chitosan/PEO in acetic aqueous solutions, but increased the fluorescent intensity of chitosan by electrostatic interactions, resulting in uniform and bead-free nanofibers with an average diameter of 150 nm.
29877707	5	5	theme	water	1107:1111	arg1	angle					1121:1125	the decreasing water contact angle	1092:1125	the decreasing water contact angle from 39° to 10°	1092:1141	The hydrophilicity of the nanofibrous films was significantly increased by the incorporation of LAE, as indicated by the decreasing water contact angle from 39° to 10°.
29877707	2	6	from	properties	299:308	arg1	solutions					344:352	acetic aqueous solutions	329:352	acetic aqueous solutions	329:352	The addition of LAE did not change the physical properties of chitosan/PEO in acetic aqueous solutions, but increased the fluorescent intensity of chitosan by electrostatic interactions, resulting in uniform and bead-free nanofibers with an average diameter of 150 nm.
29877707	4	7	theme	electrostatic	695:707	arg1	interactions					730:741	electrostatic and hydrogen bonding interactions	695:741	interactions	730:741	The formation of electrostatic and hydrogen bonding interactions induced by the LAE addition changed the inter- and intramolecular interactions between PEO and chitosan and further affected the mobility of the polymer molecules, leading to the increased crystallinity and decreased melting point.
29877707	7	8	theme	cell	1460:1463	arg1	mechanism					1421:1429	the antimicrobial mechanism	1403:1429	the antimicrobial mechanism of the nanofibrous films	1403:1454	The fluorescent staining experiments demonstrated that the antimicrobial mechanism of the nanofibrous films was cell membrane damage.
29877707	7	8	theme	cell	1460:1463	arg1	damage					1474:1479	cell membrane damage	1460:1479	cell membrane damage	1460:1479	The fluorescent staining experiments demonstrated that the antimicrobial mechanism of the nanofibrous films was cell membrane damage.
29877707	2	9	from	chitosan/PEO	313:324	arg1	solutions					344:352	acetic aqueous solutions	329:352	acetic aqueous solutions	329:352	The addition of LAE did not change the physical properties of chitosan/PEO in acetic aqueous solutions, but increased the fluorescent intensity of chitosan by electrostatic interactions, resulting in uniform and bead-free nanofibers with an average diameter of 150 nm.
29877707	2	10	theme	LAE	267:269	arg1	addition					255:262	The addition	251:262	The addition of LAE	251:269	The addition of LAE did not change the physical properties of chitosan/PEO in acetic aqueous solutions, but increased the fluorescent intensity of chitosan by electrostatic interactions, resulting in uniform and bead-free nanofibers with an average diameter of 150 nm.
29877707	4	11	theme	LAE	758:760	arg1	addition					762:769	the LAE addition	754:769	the LAE addition	754:769	The formation of electrostatic and hydrogen bonding interactions induced by the LAE addition changed the inter- and intramolecular interactions between PEO and chitosan and further affected the mobility of the polymer molecules, leading to the increased crystallinity and decreased melting point.
29877707	0	12	theme	Antimicrobial	89:101	arg1	Activity					103:110	Enhanced Antimicrobial Activity	80:110	Enhanced Antimicrobial Activity	80:110	Electrospun Chitosan/Poly(ethylene oxide)/Lauric Arginate Nanofibrous Film with Enhanced Antimicrobial Activity.
29877707	4	13	theme	molecules	896:904	arg1	mobility					872:879	the mobility	868:879	the mobility of the polymer molecules	868:904	The formation of electrostatic and hydrogen bonding interactions induced by the LAE addition changed the inter- and intramolecular interactions between PEO and chitosan and further affected the mobility of the polymer molecules, leading to the increased crystallinity and decreased melting point.
29877707	0	14	with	Film	70:73	arg1	Activity					103:110	Enhanced Antimicrobial Activity	80:110	Enhanced Antimicrobial Activity	80:110	Electrospun Chitosan/Poly(ethylene oxide)/Lauric Arginate Nanofibrous Film with Enhanced Antimicrobial Activity.
29877707	3	15	dep	Fourier	524:530	arg1	transform					532:540	transform	532:540	transform infrared spectra and thermal analysis	532:578	The Fourier transform infrared spectra and thermal analysis indicated that the LAE molecules were homogeneously dispersed within the chitosan/PEO nanofibers.
29877707	4	16	theme	bonding	722:728	arg1	interactions					730:741	electrostatic and hydrogen bonding interactions	695:741	interactions	730:741	The formation of electrostatic and hydrogen bonding interactions induced by the LAE addition changed the inter- and intramolecular interactions between PEO and chitosan and further affected the mobility of the polymer molecules, leading to the increased crystallinity and decreased melting point.
29877707	6	17	theme	antimicrobial	1324:1336	arg1	activity					1338:1345	enhanced antimicrobial activity	1315:1345	enhanced antimicrobial activity	1315:1345	Meanwhile, the chitosan/PEO/LAE nanofibrous films showed LAE concentration dependent antimicrobial activity against Escherichia coli and Staphylococcus aureus, suggesting enhanced antimicrobial activity.
29877707	5	18	theme	nanofibrous	1001:1011	arg1	films					1013:1017	the nanofibrous films	997:1017	the nanofibrous films	997:1017	The hydrophilicity of the nanofibrous films was significantly increased by the incorporation of LAE, as indicated by the decreasing water contact angle from 39° to 10°.
29877707	2	19	theme	average	492:498	arg1	diameter					500:507	an average diameter	489:507	an average diameter of 150 nm	489:517	The addition of LAE did not change the physical properties of chitosan/PEO in acetic aqueous solutions, but increased the fluorescent intensity of chitosan by electrostatic interactions, resulting in uniform and bead-free nanofibers with an average diameter of 150 nm.
29877707	6	20	theme	enhanced	1315:1322	arg1	activity					1338:1345	enhanced antimicrobial activity	1315:1345	enhanced antimicrobial activity	1315:1345	Meanwhile, the chitosan/PEO/LAE nanofibrous films showed LAE concentration dependent antimicrobial activity against Escherichia coli and Staphylococcus aureus, suggesting enhanced antimicrobial activity.
29877707	4	21	theme	inter-	783:788	arg1	interactions					809:820	the inter- and intramolecular interactions	779:820	the inter- and intramolecular interactions between PEO and chitosan	779:845	The formation of electrostatic and hydrogen bonding interactions induced by the LAE addition changed the inter- and intramolecular interactions between PEO and chitosan and further affected the mobility of the polymer molecules, leading to the increased crystallinity and decreased melting point.
29877707	5	22	from	39°	1132:1134	arg1	angle					1121:1125	the decreasing water contact angle	1092:1125	the decreasing water contact angle from 39° to 10°	1092:1141	The hydrophilicity of the nanofibrous films was significantly increased by the incorporation of LAE, as indicated by the decreasing water contact angle from 39° to 10°.
29877707	4	23	theme	interactions	730:741	arg1	formation					682:690	The formation	678:690	The formation of electrostatic and hydrogen bonding interactions induced by the LAE addition	678:769	The formation of electrostatic and hydrogen bonding interactions induced by the LAE addition changed the inter- and intramolecular interactions between PEO and chitosan and further affected the mobility of the polymer molecules, leading to the increased crystallinity and decreased melting point.
29877707	2	24	from	solutions	344:352	arg1	properties					299:308	the physical properties	286:308	the physical properties of chitosan/PEO in acetic aqueous solutions	286:352	The addition of LAE did not change the physical properties of chitosan/PEO in acetic aqueous solutions, but increased the fluorescent intensity of chitosan by electrostatic interactions, resulting in uniform and bead-free nanofibers with an average diameter of 150 nm.
29877707	6	25	theme	nanofibrous	1176:1186	arg1	films					1188:1192	the chitosan/PEO/LAE nanofibrous films	1155:1192	the chitosan/PEO/LAE nanofibrous films	1155:1192	Meanwhile, the chitosan/PEO/LAE nanofibrous films showed LAE concentration dependent antimicrobial activity against Escherichia coli and Staphylococcus aureus, suggesting enhanced antimicrobial activity.
29877707	6	25	theme	nanofibrous	1176:1186	arg1	Meanwhile					1144:1152	Meanwhile	1144:1152	Meanwhile	1144:1152	Meanwhile, the chitosan/PEO/LAE nanofibrous films showed LAE concentration dependent antimicrobial activity against Escherichia coli and Staphylococcus aureus, suggesting enhanced antimicrobial activity.
29877707	7	26	theme	antimicrobial	1407:1419	arg1	damage					1474:1479	cell membrane damage	1460:1479	cell membrane damage	1460:1479	The fluorescent staining experiments demonstrated that the antimicrobial mechanism of the nanofibrous films was cell membrane damage.
29877707	7	26	theme	antimicrobial	1407:1419	arg1	mechanism					1421:1429	the antimicrobial mechanism	1403:1429	the antimicrobial mechanism of the nanofibrous films	1403:1454	The fluorescent staining experiments demonstrated that the antimicrobial mechanism of the nanofibrous films was cell membrane damage.
29877707	2	27	theme	electrostatic	410:422	arg1	interactions					424:435	electrostatic interactions	410:435	electrostatic interactions	410:435	The addition of LAE did not change the physical properties of chitosan/PEO in acetic aqueous solutions, but increased the fluorescent intensity of chitosan by electrostatic interactions, resulting in uniform and bead-free nanofibers with an average diameter of 150 nm.
29877707	5	28	theme	LAE	1071:1073	arg1	incorporation					1054:1066	the incorporation	1050:1066	the incorporation of LAE	1050:1073	The hydrophilicity of the nanofibrous films was significantly increased by the incorporation of LAE, as indicated by the decreasing water contact angle from 39° to 10°.
29877707	6	29	theme	chitosan/PEO/LAE	1159:1174	arg1	films					1188:1192	the chitosan/PEO/LAE nanofibrous films	1155:1192	the chitosan/PEO/LAE nanofibrous films	1155:1192	Meanwhile, the chitosan/PEO/LAE nanofibrous films showed LAE concentration dependent antimicrobial activity against Escherichia coli and Staphylococcus aureus, suggesting enhanced antimicrobial activity.
29877707	6	29	theme	chitosan/PEO/LAE	1159:1174	arg1	Meanwhile					1144:1152	Meanwhile	1144:1152	Meanwhile	1144:1152	Meanwhile, the chitosan/PEO/LAE nanofibrous films showed LAE concentration dependent antimicrobial activity against Escherichia coli and Staphylococcus aureus, suggesting enhanced antimicrobial activity.
29877707	2	30	with	nanofibers	473:482	arg1	diameter					500:507	an average diameter	489:507	an average diameter of 150 nm	489:517	The addition of LAE did not change the physical properties of chitosan/PEO in acetic aqueous solutions, but increased the fluorescent intensity of chitosan by electrostatic interactions, resulting in uniform and bead-free nanofibers with an average diameter of 150 nm.
29877707	2	31	theme	chitosan	398:405	arg1	intensity					385:393	the fluorescent intensity	369:393	the fluorescent intensity of chitosan	369:405	The addition of LAE did not change the physical properties of chitosan/PEO in acetic aqueous solutions, but increased the fluorescent intensity of chitosan by electrostatic interactions, resulting in uniform and bead-free nanofibers with an average diameter of 150 nm.
29877707	6	32	theme	dependent	1219:1227	arg1	activity					1243:1250	LAE concentration dependent antimicrobial activity	1201:1250	LAE concentration dependent antimicrobial activity	1201:1250	Meanwhile, the chitosan/PEO/LAE nanofibrous films showed LAE concentration dependent antimicrobial activity against Escherichia coli and Staphylococcus aureus, suggesting enhanced antimicrobial activity.
29877707	3	33	theme	infrared	542:549	arg1	spectra					551:557	infrared spectra	542:557	infrared spectra	542:557	The Fourier transform infrared spectra and thermal analysis indicated that the LAE molecules were homogeneously dispersed within the chitosan/PEO nanofibers.
29877707	7	34	theme	fluorescent	1352:1362	arg1	experiments					1373:1383	The fluorescent staining experiments	1348:1383	The fluorescent staining experiments	1348:1383	The fluorescent staining experiments demonstrated that the antimicrobial mechanism of the nanofibrous films was cell membrane damage.
29877707	4	35	theme	melting	960:966	arg1	point					968:972	melting point	960:972	melting point	960:972	The formation of electrostatic and hydrogen bonding interactions induced by the LAE addition changed the inter- and intramolecular interactions between PEO and chitosan and further affected the mobility of the polymer molecules, leading to the increased crystallinity and decreased melting point.
29877707	5	36	theme	decreasing	1096:1105	arg1	angle					1121:1125	the decreasing water contact angle	1092:1125	the decreasing water contact angle from 39° to 10°	1092:1141	The hydrophilicity of the nanofibrous films was significantly increased by the incorporation of LAE, as indicated by the decreasing water contact angle from 39° to 10°.
29877707	7	37	theme	staining	1364:1371	arg1	experiments					1373:1383	The fluorescent staining experiments	1348:1383	The fluorescent staining experiments	1348:1383	The fluorescent staining experiments demonstrated that the antimicrobial mechanism of the nanofibrous films was cell membrane damage.
29877707	2	38	theme	uniform	451:457	arg1	nanofibers					473:482	uniform and bead-free nanofibers	451:482	uniform and bead-free nanofibers with an average diameter of 150 nm	451:517	The addition of LAE did not change the physical properties of chitosan/PEO in acetic aqueous solutions, but increased the fluorescent intensity of chitosan by electrostatic interactions, resulting in uniform and bead-free nanofibers with an average diameter of 150 nm.
29877707	2	39	theme	nm	516:517	arg1	diameter					500:507	an average diameter	489:507	an average diameter of 150 nm	489:517	The addition of LAE did not change the physical properties of chitosan/PEO in acetic aqueous solutions, but increased the fluorescent intensity of chitosan by electrostatic interactions, resulting in uniform and bead-free nanofibers with an average diameter of 150 nm.
29877707	1	40	dep	composite	186:194	arg1	oxide					151:155	ethylene oxide	142:155	ethylene oxide	142:155	In this study, chitosan/poly(ethylene oxide) (PEO)/lauric arginate (LAE) composite nanofibrous films were fabricated via electrospinning.
29877707	0	41	theme	ethylene	26:33	arg1	Chitosan/Poly					12:24	Chitosan/Poly	12:24	Chitosan/Poly	12:24	Electrospun Chitosan/Poly(ethylene oxide)/Lauric Arginate Nanofibrous Film with Enhanced Antimicrobial Activity.
29877707	0	41	theme	ethylene	26:33	arg1	oxide					35:39	ethylene oxide	26:39	ethylene oxide	26:39	Electrospun Chitosan/Poly(ethylene oxide)/Lauric Arginate Nanofibrous Film with Enhanced Antimicrobial Activity.
29877707	7	42	theme	membrane	1465:1472	arg1	mechanism					1421:1429	the antimicrobial mechanism	1403:1429	the antimicrobial mechanism of the nanofibrous films	1403:1454	The fluorescent staining experiments demonstrated that the antimicrobial mechanism of the nanofibrous films was cell membrane damage.
29877707	7	42	theme	membrane	1465:1472	arg1	damage					1474:1479	cell membrane damage	1460:1479	cell membrane damage	1460:1479	The fluorescent staining experiments demonstrated that the antimicrobial mechanism of the nanofibrous films was cell membrane damage.
29877707	2	43	theme	fluorescent	373:383	arg1	intensity					385:393	the fluorescent intensity	369:393	the fluorescent intensity of chitosan	369:405	The addition of LAE did not change the physical properties of chitosan/PEO in acetic aqueous solutions, but increased the fluorescent intensity of chitosan by electrostatic interactions, resulting in uniform and bead-free nanofibers with an average diameter of 150 nm.
29877707	0	44	theme	Arginate	49:56	arg1	Film					70:73	Arginate Nanofibrous Film	49:73	Arginate Nanofibrous Film with Enhanced Antimicrobial Activity	49:110	Electrospun Chitosan/Poly(ethylene oxide)/Lauric Arginate Nanofibrous Film with Enhanced Antimicrobial Activity.
29877707	5	45	theme	contact	1113:1119	arg1	angle					1121:1125	the decreasing water contact angle	1092:1125	the decreasing water contact angle from 39° to 10°	1092:1141	The hydrophilicity of the nanofibrous films was significantly increased by the incorporation of LAE, as indicated by the decreasing water contact angle from 39° to 10°.
29877707	3	46	theme	thermal	563:569	arg1	analysis					571:578	thermal analysis	563:578	thermal analysis	563:578	The Fourier transform infrared spectra and thermal analysis indicated that the LAE molecules were homogeneously dispersed within the chitosan/PEO nanofibers.
29877707	2	47	theme	chitosan/PEO	313:324	arg1	properties					299:308	the physical properties	286:308	the physical properties of chitosan/PEO in acetic aqueous solutions	286:352	The addition of LAE did not change the physical properties of chitosan/PEO in acetic aqueous solutions, but increased the fluorescent intensity of chitosan by electrostatic interactions, resulting in uniform and bead-free nanofibers with an average diameter of 150 nm.
29877707	1	48	theme	ethylene	142:149	arg1	oxide					151:155	ethylene oxide	142:155	ethylene oxide	142:155	In this study, chitosan/poly(ethylene oxide) (PEO)/lauric arginate (LAE) composite nanofibrous films were fabricated via electrospinning.
29877707	4	49	theme	hydrogen	713:720	arg1	interactions					730:741	electrostatic and hydrogen bonding interactions	695:741	interactions	730:741	The formation of electrostatic and hydrogen bonding interactions induced by the LAE addition changed the inter- and intramolecular interactions between PEO and chitosan and further affected the mobility of the polymer molecules, leading to the increased crystallinity and decreased melting point.
29877707	6	50	theme	antimicrobial	1229:1241	arg1	activity					1243:1250	LAE concentration dependent antimicrobial activity	1201:1250	LAE concentration dependent antimicrobial activity	1201:1250	Meanwhile, the chitosan/PEO/LAE nanofibrous films showed LAE concentration dependent antimicrobial activity against Escherichia coli and Staphylococcus aureus, suggesting enhanced antimicrobial activity.
29877707	4	51	theme	increased	922:930	arg1	crystallinity					932:944	the increased crystallinity	918:944	the increased crystallinity	918:944	The formation of electrostatic and hydrogen bonding interactions induced by the LAE addition changed the inter- and intramolecular interactions between PEO and chitosan and further affected the mobility of the polymer molecules, leading to the increased crystallinity and decreased melting point.
29877707	7	52	theme	films	1450:1454	arg1	damage					1474:1479	cell membrane damage	1460:1479	cell membrane damage	1460:1479	The fluorescent staining experiments demonstrated that the antimicrobial mechanism of the nanofibrous films was cell membrane damage.
29877707	7	52	theme	films	1450:1454	arg1	mechanism					1421:1429	the antimicrobial mechanism	1403:1429	the antimicrobial mechanism of the nanofibrous films	1403:1454	The fluorescent staining experiments demonstrated that the antimicrobial mechanism of the nanofibrous films was cell membrane damage.
29877707	1	53	theme	composite	186:194	arg1	films					208:212	chitosan/poly(ethylene oxide) (PEO)/lauric arginate (LAE) composite nanofibrous films	128:212	chitosan/poly(ethylene oxide) (PEO)/lauric arginate (LAE) composite nanofibrous films	128:212	In this study, chitosan/poly(ethylene oxide) (PEO)/lauric arginate (LAE) composite nanofibrous films were fabricated via electrospinning.
29877707	5	54	theme	films	1013:1017	arg1	hydrophilicity					979:992	The hydrophilicity	975:992	The hydrophilicity of the nanofibrous films	975:1017	The hydrophilicity of the nanofibrous films was significantly increased by the incorporation of LAE, as indicated by the decreasing water contact angle from 39° to 10°.
29877707	3	55	theme	chitosan/PEO	653:664	arg1	nanofibers					666:675	the chitosan/PEO nanofibers	649:675	the chitosan/PEO nanofibers	649:675	The Fourier transform infrared spectra and thermal analysis indicated that the LAE molecules were homogeneously dispersed within the chitosan/PEO nanofibers.
29877707	4	56	theme	intramolecular	794:807	arg1	interactions					809:820	the inter- and intramolecular interactions	779:820	the inter- and intramolecular interactions between PEO and chitosan	779:845	The formation of electrostatic and hydrogen bonding interactions induced by the LAE addition changed the inter- and intramolecular interactions between PEO and chitosan and further affected the mobility of the polymer molecules, leading to the increased crystallinity and decreased melting point.
29877707	2	57	theme	physical	290:297	arg1	properties					299:308	the physical properties	286:308	the physical properties of chitosan/PEO in acetic aqueous solutions	286:352	The addition of LAE did not change the physical properties of chitosan/PEO in acetic aqueous solutions, but increased the fluorescent intensity of chitosan by electrostatic interactions, resulting in uniform and bead-free nanofibers with an average diameter of 150 nm.
29877707	1	58	theme	nanofibrous	196:206	arg1	films					208:212	chitosan/poly(ethylene oxide) (PEO)/lauric arginate (LAE) composite nanofibrous films	128:212	chitosan/poly(ethylene oxide) (PEO)/lauric arginate (LAE) composite nanofibrous films	128:212	In this study, chitosan/poly(ethylene oxide) (PEO)/lauric arginate (LAE) composite nanofibrous films were fabricated via electrospinning.
29877707	0	59	theme	Nanofibrous	58:68	arg1	Film					70:73	Arginate Nanofibrous Film	49:73	Arginate Nanofibrous Film with Enhanced Antimicrobial Activity	49:110	Electrospun Chitosan/Poly(ethylene oxide)/Lauric Arginate Nanofibrous Film with Enhanced Antimicrobial Activity.
29877707	3	60	theme	LAE	599:601	arg1	molecules					603:611	the LAE molecules	595:611	the LAE molecules	595:611	The Fourier transform infrared spectra and thermal analysis indicated that the LAE molecules were homogeneously dispersed within the chitosan/PEO nanofibers.
29877707	2	61	theme	aqueous	336:342	arg1	solutions					344:352	acetic aqueous solutions	329:352	acetic aqueous solutions	329:352	The addition of LAE did not change the physical properties of chitosan/PEO in acetic aqueous solutions, but increased the fluorescent intensity of chitosan by electrostatic interactions, resulting in uniform and bead-free nanofibers with an average diameter of 150 nm.
31332634	5	0	contain	contains	597:604	arg2	acid					621:624	diaminopimelic acid	606:624	diaminopimelic acid (which is a diagnostic cell wall diamino acid)	606:671	The peptidoglycan was found to contains diaminopimelic acid (which is a diagnostic cell wall diamino acid), alanine, glutamic acid and lysine.
31332634	5	0	contain	contains	597:604	arg2	alanine					674:680	alanine	674:680	alanine	674:680	The peptidoglycan was found to contains diaminopimelic acid (which is a diagnostic cell wall diamino acid), alanine, glutamic acid and lysine.
31332634	5	0	contain	contains	597:604	arg2	acid					667:670	a diagnostic cell wall diamino acid	636:670	a diagnostic cell wall diamino acid	636:670	The peptidoglycan was found to contains diaminopimelic acid (which is a diagnostic cell wall diamino acid), alanine, glutamic acid and lysine.
31332634	5	0	contain	contains	597:604	arg2	acid					692:695	glutamic acid	683:695	glutamic acid	683:695	The peptidoglycan was found to contains diaminopimelic acid (which is a diagnostic cell wall diamino acid), alanine, glutamic acid and lysine.
31332634	5	0	contain	contains	597:604	arg1	peptidoglycan					570:582	The peptidoglycan	566:582	The peptidoglycan	566:582	The peptidoglycan was found to contains diaminopimelic acid (which is a diagnostic cell wall diamino acid), alanine, glutamic acid and lysine.
31332634	5	0	contain	contains	597:604	arg2	lysine					701:706	lysine	701:706	lysine	701:706	The peptidoglycan was found to contains diaminopimelic acid (which is a diagnostic cell wall diamino acid), alanine, glutamic acid and lysine.
31332634	6	1	from	DD4aT	868:872	arg1	lipids					719:724	The polar lipids diphosphatidylglycerol, phosphatidylglycerol, six unidentified glycolipids and an unidentified polar lipid	709:831	The polar lipids diphosphatidylglycerol, phosphatidylglycerol, six unidentified glycolipids and an unidentified polar lipid	709:831	The polar lipids diphosphatidylglycerol, phosphatidylglycerol, six unidentified glycolipids and an unidentified polar lipid were found to be present in strain DD4aT.
31332634	6	1	from	DD4aT	868:872	arg1	present					850:856	present	850:856	present	850:856	The polar lipids diphosphatidylglycerol, phosphatidylglycerol, six unidentified glycolipids and an unidentified polar lipid were found to be present in strain DD4aT.
31332634	9	2	theme	DD4aT	1079:1083	arg1	content					1061:1067	The DNA G+C content	1049:1067	The DNA G+C content of strain DD4aT	1049:1083	The DNA G+C content of strain DD4aT is 73.9 mol%.
31332634	9	2	theme	DD4aT	1079:1083	arg1	%					1096:1096	73.9 mol%	1088:1096	73.9 mol%	1088:1096	The DNA G+C content of strain DD4aT is 73.9 mol%.
31332634	4	3	theme	agar	553:556	arg1	medium					558:563	R2A agar medium	549:563	R2A agar medium	549:563	Strain DD4aT forms yellow colonies on R2A agar medium.
31332634	9	4	theme	73.9 mol	1088:1095	arg1	content					1061:1067	The DNA G+C content	1049:1067	The DNA G+C content of strain DD4aT	1049:1083	The DNA G+C content of strain DD4aT is 73.9 mol%.
31332634	9	4	theme	73.9 mol	1088:1095	arg1	%					1096:1096	73.9 mol%	1088:1096	73.9 mol%	1088:1096	The DNA G+C content of strain DD4aT is 73.9 mol%.
31332634	12	5	theme	genus	1570:1574	arg1	Amnibacterium					1576:1588	the genus Amnibacterium	1566:1588	the genus Amnibacterium	1566:1588	On the basis of phenotypic, genotypic, chemotaxonomic and phylogenetic analysis, DD4aT represents a novel member of the genus Amnibacterium, for which the name Amnibacterium setariae sp.
31332634	11	6	theme	kyonggiense	1385:1395	arg1	KSL51201-037T					1397:1409	A. kyonggiense KSL51201-037T	1382:1409	A. kyonggiense KSL51201-037T	1382:1409	The digital DNA-DNA hybridisation and average nucleotide identity values between strain DD4aT and A. kyonggiense KSL51201-037T were determined to be 26.1% and 82.7%.
31332634	2	7	theme	yellow	251:256	arg1	foxtail					258:264	dry yellow foxtail	247:264	dry yellow foxtail	247:264	A Gram-stain positive, short rod-shaped, aerobic, motile by means of gliding, yellow-pigmented actinobacterium, designated strain DD4aT, was isolated from dry yellow foxtail.
31332634	6	8	dep	lipids	719:724	arg1	lipids					719:724	The polar lipids diphosphatidylglycerol, phosphatidylglycerol, six unidentified glycolipids and an unidentified polar lipid	709:831	The polar lipids diphosphatidylglycerol, phosphatidylglycerol, six unidentified glycolipids and an unidentified polar lipid	709:831	The polar lipids diphosphatidylglycerol, phosphatidylglycerol, six unidentified glycolipids and an unidentified polar lipid were found to be present in strain DD4aT.
31332634	6	8	dep	lipids	719:724	arg1	phosphatidylglycerol					750:769	phosphatidylglycerol	750:769	phosphatidylglycerol	750:769	The polar lipids diphosphatidylglycerol, phosphatidylglycerol, six unidentified glycolipids and an unidentified polar lipid were found to be present in strain DD4aT.
31332634	6	8	dep	lipids	719:724	arg1	diphosphatidylglycerol					726:747	diphosphatidylglycerol	726:747	diphosphatidylglycerol	726:747	The polar lipids diphosphatidylglycerol, phosphatidylglycerol, six unidentified glycolipids and an unidentified polar lipid were found to be present in strain DD4aT.
31332634	6	8	dep	lipids	719:724	arg1	lipid					827:831	an unidentified polar lipid	805:831	an unidentified polar lipid	805:831	The polar lipids diphosphatidylglycerol, phosphatidylglycerol, six unidentified glycolipids and an unidentified polar lipid were found to be present in strain DD4aT.
31332634	6	8	dep	lipids	719:724	arg1	glycolipids					789:799	six unidentified glycolipids	772:799	six unidentified glycolipids	772:799	The polar lipids diphosphatidylglycerol, phosphatidylglycerol, six unidentified glycolipids and an unidentified polar lipid were found to be present in strain DD4aT.
31332634	6	9	theme	polar	713:717	arg1	lipids					719:724	The polar lipids diphosphatidylglycerol, phosphatidylglycerol, six unidentified glycolipids and an unidentified polar lipid	709:831	The polar lipids diphosphatidylglycerol, phosphatidylglycerol, six unidentified glycolipids and an unidentified polar lipid	709:831	The polar lipids diphosphatidylglycerol, phosphatidylglycerol, six unidentified glycolipids and an unidentified polar lipid were found to be present in strain DD4aT.
31332634	6	9	theme	polar	713:717	arg1	phosphatidylglycerol					750:769	phosphatidylglycerol	750:769	phosphatidylglycerol	750:769	The polar lipids diphosphatidylglycerol, phosphatidylglycerol, six unidentified glycolipids and an unidentified polar lipid were found to be present in strain DD4aT.
31332634	6	9	theme	polar	713:717	arg1	diphosphatidylglycerol					726:747	diphosphatidylglycerol	726:747	diphosphatidylglycerol	726:747	The polar lipids diphosphatidylglycerol, phosphatidylglycerol, six unidentified glycolipids and an unidentified polar lipid were found to be present in strain DD4aT.
31332634	6	9	theme	polar	713:717	arg1	lipid					827:831	an unidentified polar lipid	805:831	an unidentified polar lipid	805:831	The polar lipids diphosphatidylglycerol, phosphatidylglycerol, six unidentified glycolipids and an unidentified polar lipid were found to be present in strain DD4aT.
31332634	6	9	theme	polar	713:717	arg1	present					850:856	present	850:856	present	850:856	The polar lipids diphosphatidylglycerol, phosphatidylglycerol, six unidentified glycolipids and an unidentified polar lipid were found to be present in strain DD4aT.
31332634	12	10	theme	novel	1550:1554	arg1	member					1556:1561	a novel member	1548:1561	a novel member	1548:1561	On the basis of phenotypic, genotypic, chemotaxonomic and phylogenetic analysis, DD4aT represents a novel member of the genus Amnibacterium, for which the name Amnibacterium setariae sp.
31332634	11	11	theme	strain	1365:1370	arg1	DD4aT					1372:1376	strain DD4aT	1365:1376	strain DD4aT	1365:1376	The digital DNA-DNA hybridisation and average nucleotide identity values between strain DD4aT and A. kyonggiense KSL51201-037T were determined to be 26.1% and 82.7%.
31332634	5	12	theme	diamino	659:665	arg1	acid					621:624	diaminopimelic acid	606:624	diaminopimelic acid (which is a diagnostic cell wall diamino acid)	606:671	The peptidoglycan was found to contains diaminopimelic acid (which is a diagnostic cell wall diamino acid), alanine, glutamic acid and lysine.
31332634	5	12	theme	diamino	659:665	arg1	acid					667:670	a diagnostic cell wall diamino acid	636:670	a diagnostic cell wall diamino acid	636:670	The peptidoglycan was found to contains diaminopimelic acid (which is a diagnostic cell wall diamino acid), alanine, glutamic acid and lysine.
31332634	14	13	theme	Amnibacterium	1675:1687	arg1	setariae					1689:1696	Amnibacterium setariae	1675:1696	Amnibacterium setariae	1675:1696	The type strain of Amnibacterium setariae is DD4aT (= KACC 19817T = JCM 32878T).
31332634	11	14	theme	DNA-DNA	1296:1302	arg1	hybridisation					1304:1316	digital DNA-DNA hybridisation	1288:1316	digital DNA-DNA hybridisation	1288:1316	The digital DNA-DNA hybridisation and average nucleotide identity values between strain DD4aT and A. kyonggiense KSL51201-037T were determined to be 26.1% and 82.7%.
31332634	7	15	theme	strain	964:969	arg1	DD4aT					971:975	strain DD4aT	964:975	strain DD4aT	964:975	The major cellular fatty acids anteiso-C15:0 (42.9%) and iso-C16:0 (34.6%) were found in strain DD4aT.
31332634	11	16	theme	hybridisation	1304:1316	arg1	%					1437:1437	26.1%	1433:1437	26.1%	1433:1437	The digital DNA-DNA hybridisation and average nucleotide identity values between strain DD4aT and A. kyonggiense KSL51201-037T were determined to be 26.1% and 82.7%.
31332634	11	16	theme	hybridisation	1304:1316	arg1	values					1350:1355	The digital DNA-DNA hybridisation and average nucleotide identity values	1284:1355	The digital DNA-DNA hybridisation and average nucleotide identity values between strain DD4aT and A. kyonggiense KSL51201-037T	1284:1409	The digital DNA-DNA hybridisation and average nucleotide identity values between strain DD4aT and A. kyonggiense KSL51201-037T were determined to be 26.1% and 82.7%.
31332634	5	17	theme	cell	649:652	arg1	acid					621:624	diaminopimelic acid	606:624	diaminopimelic acid (which is a diagnostic cell wall diamino acid)	606:671	The peptidoglycan was found to contains diaminopimelic acid (which is a diagnostic cell wall diamino acid), alanine, glutamic acid and lysine.
31332634	5	17	theme	cell	649:652	arg1	acid					667:670	a diagnostic cell wall diamino acid	636:670	a diagnostic cell wall diamino acid	636:670	The peptidoglycan was found to contains diaminopimelic acid (which is a diagnostic cell wall diamino acid), alanine, glutamic acid and lysine.
31332634	11	18	theme	digital	1288:1294	arg1	hybridisation					1304:1316	digital DNA-DNA hybridisation	1288:1316	digital DNA-DNA hybridisation	1288:1316	The digital DNA-DNA hybridisation and average nucleotide identity values between strain DD4aT and A. kyonggiense KSL51201-037T were determined to be 26.1% and 82.7%.
31332634	2	19	attach	isolated	233:240	arg2	actinobacterium					187:201	A Gram-stain positive, short rod-shaped, aerobic, motile by means of gliding, yellow-pigmented actinobacterium	92:201	A Gram-stain positive, short rod-shaped, aerobic, motile by means of gliding, yellow-pigmented actinobacterium	92:201	A Gram-stain positive, short rod-shaped, aerobic, motile by means of gliding, yellow-pigmented actinobacterium, designated strain DD4aT, was isolated from dry yellow foxtail.
31332634	2	19	attach	isolated	233:240	arg1	foxtail					258:264	dry yellow foxtail	247:264	dry yellow foxtail	247:264	A Gram-stain positive, short rod-shaped, aerobic, motile by means of gliding, yellow-pigmented actinobacterium, designated strain DD4aT, was isolated from dry yellow foxtail.
31332634	3	20	theme	rRNA	302:305	arg1	sequences					312:320	16S rRNA gene sequences	298:320	16S rRNA gene sequences	298:320	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DD4aT is closely related to Amnibacterium soli MB78T (98.4% similarity), Amnibacterium kyonggiense KSL51201-037T (98.2%) and Amnibacterium endophyticum 1T4Z-3T (97.43%).
31332634	11	21	theme	identity	1341:1348	arg1	%					1437:1437	26.1%	1433:1437	26.1%	1433:1437	The digital DNA-DNA hybridisation and average nucleotide identity values between strain DD4aT and A. kyonggiense KSL51201-037T were determined to be 26.1% and 82.7%.
31332634	11	21	theme	identity	1341:1348	arg1	values					1350:1355	The digital DNA-DNA hybridisation and average nucleotide identity values	1284:1355	The digital DNA-DNA hybridisation and average nucleotide identity values between strain DD4aT and A. kyonggiense KSL51201-037T	1284:1409	The digital DNA-DNA hybridisation and average nucleotide identity values between strain DD4aT and A. kyonggiense KSL51201-037T were determined to be 26.1% and 82.7%.
31332634	11	22	theme	average	1322:1328	arg1	identity					1341:1348	average nucleotide identity	1322:1348	average nucleotide identity	1322:1348	The digital DNA-DNA hybridisation and average nucleotide identity values between strain DD4aT and A. kyonggiense KSL51201-037T were determined to be 26.1% and 82.7%.
31332634	4	23	from	colonies	537:544	arg1	medium					558:563	R2A agar medium	549:563	R2A agar medium	549:563	Strain DD4aT forms yellow colonies on R2A agar medium.
31332634	7	24	theme	cellular	885:892	arg1	anteiso-C15:0					906:918	anteiso-C15:0	906:918	anteiso-C15:0 (42.9%)	906:926	The major cellular fatty acids anteiso-C15:0 (42.9%) and iso-C16:0 (34.6%) were found in strain DD4aT.
31332634	7	24	theme	cellular	885:892	arg1	iso-C16:0					932:940	iso-C16:0	932:940	iso-C16:0 (34.6%)	932:948	The major cellular fatty acids anteiso-C15:0 (42.9%) and iso-C16:0 (34.6%) were found in strain DD4aT.
31332634	7	24	theme	cellular	885:892	arg1	acids					900:904	The major cellular fatty acids	875:904	The major cellular fatty acids anteiso-C15:0 (42.9%) and iso-C16:0 (34.6%)	875:948	The major cellular fatty acids anteiso-C15:0 (42.9%) and iso-C16:0 (34.6%) were found in strain DD4aT.
31332634	1	25	theme	dried	77:81	arg1	foxtail					83:89	dried foxtail	77:89	dried foxtail	77:89	nov., an endophytic actinobacterium isolated from dried foxtail.
31332634	3	26	theme	%	399:399	arg1	MB78T					388:392	Amnibacterium soli MB78T	369:392	Amnibacterium soli MB78T (98.4% similarity)	369:411	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DD4aT is closely related to Amnibacterium soli MB78T (98.4% similarity), Amnibacterium kyonggiense KSL51201-037T (98.2%) and Amnibacterium endophyticum 1T4Z-3T (97.43%).
31332634	3	26	theme	%	399:399	arg1	similarity					401:410	98.4% similarity	395:410	98.4% similarity	395:410	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DD4aT is closely related to Amnibacterium soli MB78T (98.4% similarity), Amnibacterium kyonggiense KSL51201-037T (98.2%) and Amnibacterium endophyticum 1T4Z-3T (97.43%).
31332634	0	27	theme	Amnibacterium	0:12	arg1	sp					23:24	Amnibacterium setariae sp	0:24	Amnibacterium setariae sp.	0:25	Amnibacterium setariae sp.
31332634	4	28	theme	Strain	511:516	arg1	forms					524:528	Strain DD4aT forms	511:528	Strain DD4aT forms	511:528	Strain DD4aT forms yellow colonies on R2A agar medium.
31332634	8	29	theme	respiratory	994:1004	arg1	MK-11					1032:1036	MK-11	1032:1036	MK-11	1032:1036	The predominant respiratory quinones were found to be MK-11 and MK-12.
31332634	8	29	theme	respiratory	994:1004	arg1	quinones					1006:1013	The predominant respiratory quinones	978:1013	The predominant respiratory quinones	978:1013	The predominant respiratory quinones were found to be MK-11 and MK-12.
31332634	10	30	theme	strain	1122:1127	arg1	DD4aT					1129:1133	strain DD4aT	1122:1133	strain DD4aT	1122:1133	DNA-DNA relatedness of strain DD4aT with A. soli MB78T, A. kyonggiense KSL51201-037T, and A. endophyticum 1T4Z-3T were 53.3% (± 1.1%), 47.0% (± 0.5%), and 47.9% (± 0.9%), respectively.
31332634	3	31	theme	Amnibacterium	414:426	arg1	KSL51201-037T					440:452	Amnibacterium kyonggiense KSL51201-037T	414:452	Amnibacterium kyonggiense KSL51201-037T (98.2%)	414:460	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DD4aT is closely related to Amnibacterium soli MB78T (98.4% similarity), Amnibacterium kyonggiense KSL51201-037T (98.2%) and Amnibacterium endophyticum 1T4Z-3T (97.43%).
31332634	3	31	theme	Amnibacterium	414:426	arg1	%					459:459	98.2%	455:459	98.2%	455:459	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DD4aT is closely related to Amnibacterium soli MB78T (98.4% similarity), Amnibacterium kyonggiense KSL51201-037T (98.2%) and Amnibacterium endophyticum 1T4Z-3T (97.43%).
31332634	6	32	theme	polar	821:825	arg1	lipids					719:724	The polar lipids diphosphatidylglycerol, phosphatidylglycerol, six unidentified glycolipids and an unidentified polar lipid	709:831	The polar lipids diphosphatidylglycerol, phosphatidylglycerol, six unidentified glycolipids and an unidentified polar lipid	709:831	The polar lipids diphosphatidylglycerol, phosphatidylglycerol, six unidentified glycolipids and an unidentified polar lipid were found to be present in strain DD4aT.
31332634	6	32	theme	polar	821:825	arg1	lipid					827:831	an unidentified polar lipid	805:831	an unidentified polar lipid	805:831	The polar lipids diphosphatidylglycerol, phosphatidylglycerol, six unidentified glycolipids and an unidentified polar lipid were found to be present in strain DD4aT.
31332634	12	33	theme	Amnibacterium	1610:1622	arg1	sp					1633:1634	the name Amnibacterium setariae sp	1601:1634	the name Amnibacterium setariae sp	1601:1634	On the basis of phenotypic, genotypic, chemotaxonomic and phylogenetic analysis, DD4aT represents a novel member of the genus Amnibacterium, for which the name Amnibacterium setariae sp.
31332634	3	34	theme	soli	383:386	arg1	MB78T					388:392	Amnibacterium soli MB78T	369:392	Amnibacterium soli MB78T (98.4% similarity)	369:411	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DD4aT is closely related to Amnibacterium soli MB78T (98.4% similarity), Amnibacterium kyonggiense KSL51201-037T (98.2%) and Amnibacterium endophyticum 1T4Z-3T (97.43%).
31332634	3	34	theme	soli	383:386	arg1	similarity					401:410	98.4% similarity	395:410	98.4% similarity	395:410	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DD4aT is closely related to Amnibacterium soli MB78T (98.4% similarity), Amnibacterium kyonggiense KSL51201-037T (98.2%) and Amnibacterium endophyticum 1T4Z-3T (97.43%).
31332634	2	35	dep	positive	105:112	arg1	short					115:119	short	115:119	short	115:119	A Gram-stain positive, short rod-shaped, aerobic, motile by means of gliding, yellow-pigmented actinobacterium, designated strain DD4aT, was isolated from dry yellow foxtail.
31332634	9	36	theme	DNA	1053:1055	arg1	content					1061:1067	The DNA G+C content	1049:1067	The DNA G+C content of strain DD4aT	1049:1083	The DNA G+C content of strain DD4aT is 73.9 mol%.
31332634	9	36	theme	DNA	1053:1055	arg1	%					1096:1096	73.9 mol%	1088:1096	73.9 mol%	1088:1096	The DNA G+C content of strain DD4aT is 73.9 mol%.
31332634	3	37	theme	kyonggiense	428:438	arg1	KSL51201-037T					440:452	Amnibacterium kyonggiense KSL51201-037T	414:452	Amnibacterium kyonggiense KSL51201-037T (98.2%)	414:460	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DD4aT is closely related to Amnibacterium soli MB78T (98.4% similarity), Amnibacterium kyonggiense KSL51201-037T (98.2%) and Amnibacterium endophyticum 1T4Z-3T (97.43%).
31332634	3	37	theme	kyonggiense	428:438	arg1	%					459:459	98.2%	455:459	98.2%	455:459	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DD4aT is closely related to Amnibacterium soli MB78T (98.4% similarity), Amnibacterium kyonggiense KSL51201-037T (98.2%) and Amnibacterium endophyticum 1T4Z-3T (97.43%).
31332634	14	38	theme	19817T = JCM	1715:1726	arg1	DD4aT					1701:1705	DD4aT	1701:1705	DD4aT (= KACC 19817T = JCM 32878T)	1701:1734	The type strain of Amnibacterium setariae is DD4aT (= KACC 19817T = JCM 32878T).
31332634	14	38	theme	19817T = JCM	1715:1726	arg1	32878T					1728:1733	= KACC 19817T = JCM 32878T	1708:1733	= KACC 19817T = JCM 32878T	1708:1733	The type strain of Amnibacterium setariae is DD4aT (= KACC 19817T = JCM 32878T).
31332634	5	39	theme	diaminopimelic	606:619	arg1	acid					621:624	diaminopimelic acid	606:624	diaminopimelic acid (which is a diagnostic cell wall diamino acid)	606:671	The peptidoglycan was found to contains diaminopimelic acid (which is a diagnostic cell wall diamino acid), alanine, glutamic acid and lysine.
31332634	5	39	theme	diaminopimelic	606:619	arg1	acid					667:670	a diagnostic cell wall diamino acid	636:670	a diagnostic cell wall diamino acid	636:670	The peptidoglycan was found to contains diaminopimelic acid (which is a diagnostic cell wall diamino acid), alanine, glutamic acid and lysine.
31332634	5	39	theme	diaminopimelic	606:619	arg1	alanine					674:680	alanine	674:680	alanine	674:680	The peptidoglycan was found to contains diaminopimelic acid (which is a diagnostic cell wall diamino acid), alanine, glutamic acid and lysine.
31332634	5	39	theme	diaminopimelic	606:619	arg1	acid					692:695	glutamic acid	683:695	glutamic acid	683:695	The peptidoglycan was found to contains diaminopimelic acid (which is a diagnostic cell wall diamino acid), alanine, glutamic acid and lysine.
31332634	5	39	theme	diaminopimelic	606:619	arg1	lysine					701:706	lysine	701:706	lysine	701:706	The peptidoglycan was found to contains diaminopimelic acid (which is a diagnostic cell wall diamino acid), alanine, glutamic acid and lysine.
31332634	9	40	theme	strain	1072:1077	arg1	DD4aT					1079:1083	strain DD4aT	1072:1083	strain DD4aT	1072:1083	The DNA G+C content of strain DD4aT is 73.9 mol%.
31332634	3	41	theme	Amnibacterium	466:478	arg1	1T4Z-3T					493:499	Amnibacterium endophyticum 1T4Z-3T	466:499	Amnibacterium endophyticum 1T4Z-3T (97.43%)	466:508	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DD4aT is closely related to Amnibacterium soli MB78T (98.4% similarity), Amnibacterium kyonggiense KSL51201-037T (98.2%) and Amnibacterium endophyticum 1T4Z-3T (97.43%).
31332634	3	41	theme	Amnibacterium	466:478	arg1	%					507:507	97.43%	502:507	97.43%	502:507	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DD4aT is closely related to Amnibacterium soli MB78T (98.4% similarity), Amnibacterium kyonggiense KSL51201-037T (98.2%) and Amnibacterium endophyticum 1T4Z-3T (97.43%).
31332634	6	42	theme	strain	861:866	arg1	DD4aT					868:872	strain DD4aT	861:872	strain DD4aT	861:872	The polar lipids diphosphatidylglycerol, phosphatidylglycerol, six unidentified glycolipids and an unidentified polar lipid were found to be present in strain DD4aT.
31332634	2	43	theme	positive	105:112	arg1	actinobacterium					187:201	A Gram-stain positive, short rod-shaped, aerobic, motile by means of gliding, yellow-pigmented actinobacterium	92:201	A Gram-stain positive, short rod-shaped, aerobic, motile by means of gliding, yellow-pigmented actinobacterium	92:201	A Gram-stain positive, short rod-shaped, aerobic, motile by means of gliding, yellow-pigmented actinobacterium, designated strain DD4aT, was isolated from dry yellow foxtail.
31332634	10	44	theme	DNA-DNA	1099:1105	arg1	relatedness					1107:1117	DNA-DNA relatedness	1099:1117	DNA-DNA relatedness of strain DD4aT with A. soli MB78T, A. kyonggiense KSL51201-037T, and A. endophyticum 1T4Z-3T	1099:1211	DNA-DNA relatedness of strain DD4aT with A. soli MB78T, A. kyonggiense KSL51201-037T, and A. endophyticum 1T4Z-3T were 53.3% (± 1.1%), 47.0% (± 0.5%), and 47.9% (± 0.9%), respectively.
31332634	12	45	theme	Amnibacterium	1576:1588	arg1	member					1556:1561	a novel member	1548:1561	a novel member	1548:1561	On the basis of phenotypic, genotypic, chemotaxonomic and phylogenetic analysis, DD4aT represents a novel member of the genus Amnibacterium, for which the name Amnibacterium setariae sp.
31332634	4	46	theme	R2A	549:551	arg1	medium					558:563	R2A agar medium	549:563	R2A agar medium	549:563	Strain DD4aT forms yellow colonies on R2A agar medium.
31332634	6	47	from	present	850:856	arg1	DD4aT					868:872	strain DD4aT	861:872	strain DD4aT	861:872	The polar lipids diphosphatidylglycerol, phosphatidylglycerol, six unidentified glycolipids and an unidentified polar lipid were found to be present in strain DD4aT.
31332634	14	48	theme	= KACC	1708:1713	arg1	DD4aT					1701:1705	DD4aT	1701:1705	DD4aT (= KACC 19817T = JCM 32878T)	1701:1734	The type strain of Amnibacterium setariae is DD4aT (= KACC 19817T = JCM 32878T).
31332634	14	48	theme	= KACC	1708:1713	arg1	32878T					1728:1733	= KACC 19817T = JCM 32878T	1708:1733	= KACC 19817T = JCM 32878T	1708:1733	The type strain of Amnibacterium setariae is DD4aT (= KACC 19817T = JCM 32878T).
31332634	6	49	located	present	850:856	arg1	DD4aT					868:872	strain DD4aT	861:872	strain DD4aT	861:872	The polar lipids diphosphatidylglycerol, phosphatidylglycerol, six unidentified glycolipids and an unidentified polar lipid were found to be present in strain DD4aT.
31332634	6	49	located	present	850:856	arg2	lipid					827:831	an unidentified polar lipid	805:831	an unidentified polar lipid	805:831	The polar lipids diphosphatidylglycerol, phosphatidylglycerol, six unidentified glycolipids and an unidentified polar lipid were found to be present in strain DD4aT.
31332634	6	49	located	present	850:856	arg2	diphosphatidylglycerol					726:747	diphosphatidylglycerol	726:747	diphosphatidylglycerol	726:747	The polar lipids diphosphatidylglycerol, phosphatidylglycerol, six unidentified glycolipids and an unidentified polar lipid were found to be present in strain DD4aT.
31332634	6	49	located	present	850:856	arg2	lipids					719:724	The polar lipids diphosphatidylglycerol, phosphatidylglycerol, six unidentified glycolipids and an unidentified polar lipid	709:831	The polar lipids diphosphatidylglycerol, phosphatidylglycerol, six unidentified glycolipids and an unidentified polar lipid	709:831	The polar lipids diphosphatidylglycerol, phosphatidylglycerol, six unidentified glycolipids and an unidentified polar lipid were found to be present in strain DD4aT.
31332634	6	49	located	present	850:856	arg2	present					850:856	present	850:856	present	850:856	The polar lipids diphosphatidylglycerol, phosphatidylglycerol, six unidentified glycolipids and an unidentified polar lipid were found to be present in strain DD4aT.
31332634	6	49	located	present	850:856	arg2	phosphatidylglycerol					750:769	phosphatidylglycerol	750:769	phosphatidylglycerol	750:769	The polar lipids diphosphatidylglycerol, phosphatidylglycerol, six unidentified glycolipids and an unidentified polar lipid were found to be present in strain DD4aT.
31332634	12	50	dep	analysis	1521:1528	arg1	the					1453:1455	the	1453:1455	the	1453:1455	On the basis of phenotypic, genotypic, chemotaxonomic and phylogenetic analysis, DD4aT represents a novel member of the genus Amnibacterium, for which the name Amnibacterium setariae sp.
31332634	12	50	dep	analysis	1521:1528	arg1	basis					1457:1461	basis	1457:1461	basis	1457:1461	On the basis of phenotypic, genotypic, chemotaxonomic and phylogenetic analysis, DD4aT represents a novel member of the genus Amnibacterium, for which the name Amnibacterium setariae sp.
31332634	5	51	theme	wall	654:657	arg1	acid					621:624	diaminopimelic acid	606:624	diaminopimelic acid (which is a diagnostic cell wall diamino acid)	606:671	The peptidoglycan was found to contains diaminopimelic acid (which is a diagnostic cell wall diamino acid), alanine, glutamic acid and lysine.
31332634	5	51	theme	wall	654:657	arg1	acid					667:670	a diagnostic cell wall diamino acid	636:670	a diagnostic cell wall diamino acid	636:670	The peptidoglycan was found to contains diaminopimelic acid (which is a diagnostic cell wall diamino acid), alanine, glutamic acid and lysine.
31332634	11	52	theme	A.	1382:1383	arg1	KSL51201-037T					1397:1409	A. kyonggiense KSL51201-037T	1382:1409	A. kyonggiense KSL51201-037T	1382:1409	The digital DNA-DNA hybridisation and average nucleotide identity values between strain DD4aT and A. kyonggiense KSL51201-037T were determined to be 26.1% and 82.7%.
31332634	14	53	theme	setariae	1689:1696	arg1	DD4aT					1701:1705	DD4aT	1701:1705	DD4aT (= KACC 19817T = JCM 32878T)	1701:1734	The type strain of Amnibacterium setariae is DD4aT (= KACC 19817T = JCM 32878T).
31332634	14	53	theme	setariae	1689:1696	arg1	strain					1665:1670	The type strain	1656:1670	The type strain of Amnibacterium setariae	1656:1696	The type strain of Amnibacterium setariae is DD4aT (= KACC 19817T = JCM 32878T).
31332634	3	54	theme	Phylogenetic	267:278	arg1	analysis					280:287	Phylogenetic analysis	267:287	Phylogenetic analysis based on 16S rRNA gene sequences	267:320	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DD4aT is closely related to Amnibacterium soli MB78T (98.4% similarity), Amnibacterium kyonggiense KSL51201-037T (98.2%) and Amnibacterium endophyticum 1T4Z-3T (97.43%).
31332634	2	55	theme	strain	215:220	arg1	DD4aT					222:226	strain DD4aT	215:226	strain DD4aT	215:226	A Gram-stain positive, short rod-shaped, aerobic, motile by means of gliding, yellow-pigmented actinobacterium, designated strain DD4aT, was isolated from dry yellow foxtail.
31332634	14	56	theme	type	1660:1663	arg1	DD4aT					1701:1705	DD4aT	1701:1705	DD4aT (= KACC 19817T = JCM 32878T)	1701:1734	The type strain of Amnibacterium setariae is DD4aT (= KACC 19817T = JCM 32878T).
31332634	14	56	theme	type	1660:1663	arg1	strain					1665:1670	The type strain	1656:1670	The type strain of Amnibacterium setariae	1656:1696	The type strain of Amnibacterium setariae is DD4aT (= KACC 19817T = JCM 32878T).
31332634	3	57	theme	16S	298:300	arg1	sequences					312:320	16S rRNA gene sequences	298:320	16S rRNA gene sequences	298:320	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DD4aT is closely related to Amnibacterium soli MB78T (98.4% similarity), Amnibacterium kyonggiense KSL51201-037T (98.2%) and Amnibacterium endophyticum 1T4Z-3T (97.43%).
31332634	7	58	dep	acids	900:904	arg1	anteiso-C15:0					906:918	anteiso-C15:0	906:918	anteiso-C15:0 (42.9%)	906:926	The major cellular fatty acids anteiso-C15:0 (42.9%) and iso-C16:0 (34.6%) were found in strain DD4aT.
31332634	7	58	dep	acids	900:904	arg1	%					925:925	42.9%	921:925	42.9%	921:925	The major cellular fatty acids anteiso-C15:0 (42.9%) and iso-C16:0 (34.6%) were found in strain DD4aT.
31332634	7	58	dep	acids	900:904	arg1	iso-C16:0					932:940	iso-C16:0	932:940	iso-C16:0 (34.6%)	932:948	The major cellular fatty acids anteiso-C15:0 (42.9%) and iso-C16:0 (34.6%) were found in strain DD4aT.
31332634	7	58	dep	acids	900:904	arg1	%					947:947	34.6%	943:947	34.6%	943:947	The major cellular fatty acids anteiso-C15:0 (42.9%) and iso-C16:0 (34.6%) were found in strain DD4aT.
31332634	7	58	dep	acids	900:904	arg1	acids					900:904	The major cellular fatty acids	875:904	The major cellular fatty acids anteiso-C15:0 (42.9%) and iso-C16:0 (34.6%)	875:948	The major cellular fatty acids anteiso-C15:0 (42.9%) and iso-C16:0 (34.6%) were found in strain DD4aT.
31332634	5	59	theme	diagnostic	638:647	arg1	acid					621:624	diaminopimelic acid	606:624	diaminopimelic acid (which is a diagnostic cell wall diamino acid)	606:671	The peptidoglycan was found to contains diaminopimelic acid (which is a diagnostic cell wall diamino acid), alanine, glutamic acid and lysine.
31332634	5	59	theme	diagnostic	638:647	arg1	acid					667:670	a diagnostic cell wall diamino acid	636:670	a diagnostic cell wall diamino acid	636:670	The peptidoglycan was found to contains diaminopimelic acid (which is a diagnostic cell wall diamino acid), alanine, glutamic acid and lysine.
31332634	2	60	theme	dry	247:249	arg1	foxtail					258:264	dry yellow foxtail	247:264	dry yellow foxtail	247:264	A Gram-stain positive, short rod-shaped, aerobic, motile by means of gliding, yellow-pigmented actinobacterium, designated strain DD4aT, was isolated from dry yellow foxtail.
31332634	12	61	theme	phylogenetic	1508:1519	arg1	analysis					1521:1528	phenotypic, genotypic, chemotaxonomic and phylogenetic analysis	1466:1528	phenotypic, genotypic, chemotaxonomic and phylogenetic analysis	1466:1528	On the basis of phenotypic, genotypic, chemotaxonomic and phylogenetic analysis, DD4aT represents a novel member of the genus Amnibacterium, for which the name Amnibacterium setariae sp.
31332634	10	62	theme	DD4aT	1129:1133	arg1	relatedness					1107:1117	DNA-DNA relatedness	1099:1117	DNA-DNA relatedness of strain DD4aT with A. soli MB78T, A. kyonggiense KSL51201-037T, and A. endophyticum 1T4Z-3T	1099:1211	DNA-DNA relatedness of strain DD4aT with A. soli MB78T, A. kyonggiense KSL51201-037T, and A. endophyticum 1T4Z-3T were 53.3% (± 1.1%), 47.0% (± 0.5%), and 47.9% (± 0.9%), respectively.
31332634	11	63	theme	nucleotide	1330:1339	arg1	identity					1341:1348	average nucleotide identity	1322:1348	average nucleotide identity	1322:1348	The digital DNA-DNA hybridisation and average nucleotide identity values between strain DD4aT and A. kyonggiense KSL51201-037T were determined to be 26.1% and 82.7%.
31332634	12	64	theme	genotypic	1478:1486	arg1	analysis					1521:1528	phenotypic, genotypic, chemotaxonomic and phylogenetic analysis	1466:1528	phenotypic, genotypic, chemotaxonomic and phylogenetic analysis	1466:1528	On the basis of phenotypic, genotypic, chemotaxonomic and phylogenetic analysis, DD4aT represents a novel member of the genus Amnibacterium, for which the name Amnibacterium setariae sp.
31332634	3	65	theme	gene	307:310	arg1	sequences					312:320	16S rRNA gene sequences	298:320	16S rRNA gene sequences	298:320	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DD4aT is closely related to Amnibacterium soli MB78T (98.4% similarity), Amnibacterium kyonggiense KSL51201-037T (98.2%) and Amnibacterium endophyticum 1T4Z-3T (97.43%).
31332634	12	66	theme	chemotaxonomic	1489:1502	arg1	analysis					1521:1528	phenotypic, genotypic, chemotaxonomic and phylogenetic analysis	1466:1528	phenotypic, genotypic, chemotaxonomic and phylogenetic analysis	1466:1528	On the basis of phenotypic, genotypic, chemotaxonomic and phylogenetic analysis, DD4aT represents a novel member of the genus Amnibacterium, for which the name Amnibacterium setariae sp.
31332634	3	67	theme	strain	334:339	arg1	DD4aT					341:345	strain DD4aT	334:345	strain DD4aT	334:345	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DD4aT is closely related to Amnibacterium soli MB78T (98.4% similarity), Amnibacterium kyonggiense KSL51201-037T (98.2%) and Amnibacterium endophyticum 1T4Z-3T (97.43%).
31332634	7	68	located	found	955:959	arg2	anteiso-C15:0					906:918	anteiso-C15:0	906:918	anteiso-C15:0 (42.9%)	906:926	The major cellular fatty acids anteiso-C15:0 (42.9%) and iso-C16:0 (34.6%) were found in strain DD4aT.
31332634	7	68	located	found	955:959	arg2	iso-C16:0					932:940	iso-C16:0	932:940	iso-C16:0 (34.6%)	932:948	The major cellular fatty acids anteiso-C15:0 (42.9%) and iso-C16:0 (34.6%) were found in strain DD4aT.
31332634	7	68	located	found	955:959	arg2	acids					900:904	The major cellular fatty acids	875:904	The major cellular fatty acids anteiso-C15:0 (42.9%) and iso-C16:0 (34.6%)	875:948	The major cellular fatty acids anteiso-C15:0 (42.9%) and iso-C16:0 (34.6%) were found in strain DD4aT.
31332634	7	68	located	found	955:959	arg1	DD4aT					971:975	strain DD4aT	964:975	strain DD4aT	964:975	The major cellular fatty acids anteiso-C15:0 (42.9%) and iso-C16:0 (34.6%) were found in strain DD4aT.
31332634	6	69	attach	present	850:856	arg1	DD4aT					868:872	strain DD4aT	861:872	strain DD4aT	861:872	The polar lipids diphosphatidylglycerol, phosphatidylglycerol, six unidentified glycolipids and an unidentified polar lipid were found to be present in strain DD4aT.
31332634	6	69	attach	present	850:856	arg2	lipid					827:831	an unidentified polar lipid	805:831	an unidentified polar lipid	805:831	The polar lipids diphosphatidylglycerol, phosphatidylglycerol, six unidentified glycolipids and an unidentified polar lipid were found to be present in strain DD4aT.
31332634	6	69	attach	present	850:856	arg2	diphosphatidylglycerol					726:747	diphosphatidylglycerol	726:747	diphosphatidylglycerol	726:747	The polar lipids diphosphatidylglycerol, phosphatidylglycerol, six unidentified glycolipids and an unidentified polar lipid were found to be present in strain DD4aT.
31332634	6	69	attach	present	850:856	arg2	lipids					719:724	The polar lipids diphosphatidylglycerol, phosphatidylglycerol, six unidentified glycolipids and an unidentified polar lipid	709:831	The polar lipids diphosphatidylglycerol, phosphatidylglycerol, six unidentified glycolipids and an unidentified polar lipid	709:831	The polar lipids diphosphatidylglycerol, phosphatidylglycerol, six unidentified glycolipids and an unidentified polar lipid were found to be present in strain DD4aT.
31332634	6	69	attach	present	850:856	arg2	present					850:856	present	850:856	present	850:856	The polar lipids diphosphatidylglycerol, phosphatidylglycerol, six unidentified glycolipids and an unidentified polar lipid were found to be present in strain DD4aT.
31332634	6	69	attach	present	850:856	arg2	phosphatidylglycerol					750:769	phosphatidylglycerol	750:769	phosphatidylglycerol	750:769	The polar lipids diphosphatidylglycerol, phosphatidylglycerol, six unidentified glycolipids and an unidentified polar lipid were found to be present in strain DD4aT.
31332634	0	70	theme	setariae	14:21	arg1	sp					23:24	Amnibacterium setariae sp	0:24	Amnibacterium setariae sp.	0:25	Amnibacterium setariae sp.
31332634	8	71	theme	predominant	982:992	arg1	MK-11					1032:1036	MK-11	1032:1036	MK-11	1032:1036	The predominant respiratory quinones were found to be MK-11 and MK-12.
31332634	8	71	theme	predominant	982:992	arg1	quinones					1006:1013	The predominant respiratory quinones	978:1013	The predominant respiratory quinones	978:1013	The predominant respiratory quinones were found to be MK-11 and MK-12.
31332634	7	72	theme	fatty	894:898	arg1	anteiso-C15:0					906:918	anteiso-C15:0	906:918	anteiso-C15:0 (42.9%)	906:926	The major cellular fatty acids anteiso-C15:0 (42.9%) and iso-C16:0 (34.6%) were found in strain DD4aT.
31332634	7	72	theme	fatty	894:898	arg1	iso-C16:0					932:940	iso-C16:0	932:940	iso-C16:0 (34.6%)	932:948	The major cellular fatty acids anteiso-C15:0 (42.9%) and iso-C16:0 (34.6%) were found in strain DD4aT.
31332634	7	72	theme	fatty	894:898	arg1	acids					900:904	The major cellular fatty acids	875:904	The major cellular fatty acids anteiso-C15:0 (42.9%) and iso-C16:0 (34.6%)	875:948	The major cellular fatty acids anteiso-C15:0 (42.9%) and iso-C16:0 (34.6%) were found in strain DD4aT.
31332634	3	73	dep	Amnibacterium	466:478	arg1	endophyticum					480:491	endophyticum	480:491	endophyticum	480:491	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DD4aT is closely related to Amnibacterium soli MB78T (98.4% similarity), Amnibacterium kyonggiense KSL51201-037T (98.2%) and Amnibacterium endophyticum 1T4Z-3T (97.43%).
31332634	12	74	theme	phenotypic	1466:1475	arg1	analysis					1521:1528	phenotypic, genotypic, chemotaxonomic and phylogenetic analysis	1466:1528	phenotypic, genotypic, chemotaxonomic and phylogenetic analysis	1466:1528	On the basis of phenotypic, genotypic, chemotaxonomic and phylogenetic analysis, DD4aT represents a novel member of the genus Amnibacterium, for which the name Amnibacterium setariae sp.
31332634	3	75	theme	Amnibacterium	369:381	arg1	MB78T					388:392	Amnibacterium soli MB78T	369:392	Amnibacterium soli MB78T (98.4% similarity)	369:411	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DD4aT is closely related to Amnibacterium soli MB78T (98.4% similarity), Amnibacterium kyonggiense KSL51201-037T (98.2%) and Amnibacterium endophyticum 1T4Z-3T (97.43%).
31332634	3	75	theme	Amnibacterium	369:381	arg1	similarity					401:410	98.4% similarity	395:410	98.4% similarity	395:410	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DD4aT is closely related to Amnibacterium soli MB78T (98.4% similarity), Amnibacterium kyonggiense KSL51201-037T (98.2%) and Amnibacterium endophyticum 1T4Z-3T (97.43%).
31332634	4	76	theme	DD4aT	518:522	arg1	forms					524:528	Strain DD4aT forms	511:528	Strain DD4aT forms	511:528	Strain DD4aT forms yellow colonies on R2A agar medium.
31332634	7	77	theme	major	879:883	arg1	anteiso-C15:0					906:918	anteiso-C15:0	906:918	anteiso-C15:0 (42.9%)	906:926	The major cellular fatty acids anteiso-C15:0 (42.9%) and iso-C16:0 (34.6%) were found in strain DD4aT.
31332634	7	77	theme	major	879:883	arg1	iso-C16:0					932:940	iso-C16:0	932:940	iso-C16:0 (34.6%)	932:948	The major cellular fatty acids anteiso-C15:0 (42.9%) and iso-C16:0 (34.6%) were found in strain DD4aT.
31332634	7	77	theme	major	879:883	arg1	acids					900:904	The major cellular fatty acids	875:904	The major cellular fatty acids anteiso-C15:0 (42.9%) and iso-C16:0 (34.6%)	875:948	The major cellular fatty acids anteiso-C15:0 (42.9%) and iso-C16:0 (34.6%) were found in strain DD4aT.
31332634	10	78	with	relatedness	1107:1117	arg1	endophyticum					1192:1203	endophyticum	1192:1203	endophyticum	1192:1203	DNA-DNA relatedness of strain DD4aT with A. soli MB78T, A. kyonggiense KSL51201-037T, and A. endophyticum 1T4Z-3T were 53.3% (± 1.1%), 47.0% (± 0.5%), and 47.9% (± 0.9%), respectively.
31332634	10	78	with	relatedness	1107:1117	arg1	KSL51201-037T					1170:1182	KSL51201-037T	1170:1182	KSL51201-037T	1170:1182	DNA-DNA relatedness of strain DD4aT with A. soli MB78T, A. kyonggiense KSL51201-037T, and A. endophyticum 1T4Z-3T were 53.3% (± 1.1%), 47.0% (± 0.5%), and 47.9% (± 0.9%), respectively.
31332634	10	78	with	relatedness	1107:1117	arg1	MB78T					1148:1152	MB78T	1148:1152	MB78T	1148:1152	DNA-DNA relatedness of strain DD4aT with A. soli MB78T, A. kyonggiense KSL51201-037T, and A. endophyticum 1T4Z-3T were 53.3% (± 1.1%), 47.0% (± 0.5%), and 47.9% (± 0.9%), respectively.
31332634	3	79	theme	98.4	395:398	arg1	%					399:399	%	399:399	%	399:399	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DD4aT is closely related to Amnibacterium soli MB78T (98.4% similarity), Amnibacterium kyonggiense KSL51201-037T (98.2%) and Amnibacterium endophyticum 1T4Z-3T (97.43%).
31332634	5	80	theme	glutamic	683:690	arg1	acid					621:624	diaminopimelic acid	606:624	diaminopimelic acid (which is a diagnostic cell wall diamino acid)	606:671	The peptidoglycan was found to contains diaminopimelic acid (which is a diagnostic cell wall diamino acid), alanine, glutamic acid and lysine.
31332634	5	80	theme	glutamic	683:690	arg1	acid					692:695	glutamic acid	683:695	glutamic acid	683:695	The peptidoglycan was found to contains diaminopimelic acid (which is a diagnostic cell wall diamino acid), alanine, glutamic acid and lysine.
31332634	6	81	theme	unidentified	808:819	arg1	lipids					719:724	The polar lipids diphosphatidylglycerol, phosphatidylglycerol, six unidentified glycolipids and an unidentified polar lipid	709:831	The polar lipids diphosphatidylglycerol, phosphatidylglycerol, six unidentified glycolipids and an unidentified polar lipid	709:831	The polar lipids diphosphatidylglycerol, phosphatidylglycerol, six unidentified glycolipids and an unidentified polar lipid were found to be present in strain DD4aT.
31332634	6	81	theme	unidentified	808:819	arg1	lipid					827:831	an unidentified polar lipid	805:831	an unidentified polar lipid	805:831	The polar lipids diphosphatidylglycerol, phosphatidylglycerol, six unidentified glycolipids and an unidentified polar lipid were found to be present in strain DD4aT.
31332634	12	82	theme	name	1605:1608	arg1	sp					1633:1634	the name Amnibacterium setariae sp	1601:1634	the name Amnibacterium setariae sp	1601:1634	On the basis of phenotypic, genotypic, chemotaxonomic and phylogenetic analysis, DD4aT represents a novel member of the genus Amnibacterium, for which the name Amnibacterium setariae sp.
31332634	2	83	dep	rod-shaped	121:130	arg1	yellow-pigmented					170:185	yellow-pigmented	170:185	yellow-pigmented	170:185	A Gram-stain positive, short rod-shaped, aerobic, motile by means of gliding, yellow-pigmented actinobacterium, designated strain DD4aT, was isolated from dry yellow foxtail.
31332634	2	83	dep	rod-shaped	121:130	arg1	motile					142:147	motile	142:147	motile	142:147	A Gram-stain positive, short rod-shaped, aerobic, motile by means of gliding, yellow-pigmented actinobacterium, designated strain DD4aT, was isolated from dry yellow foxtail.
31332634	2	83	dep	rod-shaped	121:130	arg1	aerobic					133:139	aerobic	133:139	aerobic	133:139	A Gram-stain positive, short rod-shaped, aerobic, motile by means of gliding, yellow-pigmented actinobacterium, designated strain DD4aT, was isolated from dry yellow foxtail.
31332634	1	84	theme	endophytic	36:45	arg1	actinobacterium					47:61	an endophytic actinobacterium	33:61	an endophytic actinobacterium	33:61	nov., an endophytic actinobacterium isolated from dried foxtail.
31332634	1	84	theme	endophytic	36:45	arg1	nov.					27:30	nov.	27:30	nov.	27:30	nov., an endophytic actinobacterium isolated from dried foxtail.
31332634	12	85	theme	setariae	1624:1631	arg1	sp					1633:1634	the name Amnibacterium setariae sp	1601:1634	the name Amnibacterium setariae sp	1601:1634	On the basis of phenotypic, genotypic, chemotaxonomic and phylogenetic analysis, DD4aT represents a novel member of the genus Amnibacterium, for which the name Amnibacterium setariae sp.
31332634	9	86	theme	G+C	1057:1059	arg1	content					1061:1067	The DNA G+C content	1049:1067	The DNA G+C content of strain DD4aT	1049:1083	The DNA G+C content of strain DD4aT is 73.9 mol%.
31332634	9	86	theme	G+C	1057:1059	arg1	%					1096:1096	73.9 mol%	1088:1096	73.9 mol%	1088:1096	The DNA G+C content of strain DD4aT is 73.9 mol%.
31332634	6	87	theme	unidentified	776:787	arg1	glycolipids					789:799	six unidentified glycolipids	772:799	six unidentified glycolipids	772:799	The polar lipids diphosphatidylglycerol, phosphatidylglycerol, six unidentified glycolipids and an unidentified polar lipid were found to be present in strain DD4aT.
31332634	2	88	theme	rod-shaped	121:130	arg1	actinobacterium					187:201	A Gram-stain positive, short rod-shaped, aerobic, motile by means of gliding, yellow-pigmented actinobacterium	92:201	A Gram-stain positive, short rod-shaped, aerobic, motile by means of gliding, yellow-pigmented actinobacterium	92:201	A Gram-stain positive, short rod-shaped, aerobic, motile by means of gliding, yellow-pigmented actinobacterium, designated strain DD4aT, was isolated from dry yellow foxtail.
31601450	2	0	theme	actinobacterial	105:119	arg1	MS1-9T					130:135	MS1-9T	130:135	MS1-9T	130:135	Two novel actinobacterial strains, MS1-9T and NGC1-4, were isolated from roots of Musa (ABB) cv.
31601450	2	0	theme	actinobacterial	105:119	arg1	strains					121:127	Two novel actinobacterial strains	95:127	Two novel actinobacterial strains	95:127	Two novel actinobacterial strains, MS1-9T and NGC1-4, were isolated from roots of Musa (ABB) cv.
31601450	2	0	theme	actinobacterial	105:119	arg1	NGC1-4					141:146	NGC1-4	141:146	NGC1-4	141:146	Two novel actinobacterial strains, MS1-9T and NGC1-4, were isolated from roots of Musa (ABB) cv.
31601450	5	1	theme	B	411:411	arg1	gene					420:423	gyrase subunit B (gyrB) gene	396:423	gyrase subunit B (gyrB) gene	396:423	Comparative analysis of 16S rRNA gene (98.0 to 98.9% similarity), gyrase subunit B (gyrB) gene and whole-genome sequences emphasised that the strains MS1-9T and NGC1-4 showed closely related with Micromonospora peucetia DSM 43363T, M. krabiensis JCM 12869T and M. avicenniae DSM 45758T, respectively.
31601450	5	2	with	related	513:519	arg1	45758T					609:614	M. avicenniae DSM 45758T	591:614	M. avicenniae DSM 45758T	591:614	Comparative analysis of 16S rRNA gene (98.0 to 98.9% similarity), gyrase subunit B (gyrB) gene and whole-genome sequences emphasised that the strains MS1-9T and NGC1-4 showed closely related with Micromonospora peucetia DSM 43363T, M. krabiensis JCM 12869T and M. avicenniae DSM 45758T, respectively.
31601450	5	2	with	related	513:519	arg1	12869T					580:585	M. krabiensis JCM 12869T	562:585	M. krabiensis JCM 12869T	562:585	Comparative analysis of 16S rRNA gene (98.0 to 98.9% similarity), gyrase subunit B (gyrB) gene and whole-genome sequences emphasised that the strains MS1-9T and NGC1-4 showed closely related with Micromonospora peucetia DSM 43363T, M. krabiensis JCM 12869T and M. avicenniae DSM 45758T, respectively.
31601450	5	2	with	related	513:519	arg1	43363T					554:559	Micromonospora peucetia DSM 43363T	526:559	Micromonospora peucetia DSM 43363T	526:559	Comparative analysis of 16S rRNA gene (98.0 to 98.9% similarity), gyrase subunit B (gyrB) gene and whole-genome sequences emphasised that the strains MS1-9T and NGC1-4 showed closely related with Micromonospora peucetia DSM 43363T, M. krabiensis JCM 12869T and M. avicenniae DSM 45758T, respectively.
31601450	13	3	theme	genus	1431:1435	arg1	Micromonospora					1437:1450	the genus Micromonospora	1427:1450	the genus Micromonospora for which the name Micromonospora musae sp	1427:1493	Draft genome sequences indicated by ANI values and digital DNA-DNA hybridisation analysis asserted that the strains MS1-9T and NGC1-4 should be represented as a novel species within the genus Micromonospora for which the name Micromonospora musae sp.
31601450	3	4	theme	ABB	255:257	arg1	cv					260:261	Musa (ABB) cv	249:261	Musa (ABB) cv	249:261	'Kluai Namwa', collected from Chachoengsao province, and Musa (ABB) cv.
31601450	2	5	theme	novel	99:103	arg1	MS1-9T					130:135	MS1-9T	130:135	MS1-9T	130:135	Two novel actinobacterial strains, MS1-9T and NGC1-4, were isolated from roots of Musa (ABB) cv.
31601450	2	5	theme	novel	99:103	arg1	strains					121:127	Two novel actinobacterial strains	95:127	Two novel actinobacterial strains	95:127	Two novel actinobacterial strains, MS1-9T and NGC1-4, were isolated from roots of Musa (ABB) cv.
31601450	2	5	theme	novel	99:103	arg1	NGC1-4					141:146	NGC1-4	141:146	NGC1-4	141:146	Two novel actinobacterial strains, MS1-9T and NGC1-4, were isolated from roots of Musa (ABB) cv.
31601450	6	6	theme	cell-wall	695:703	arg1	peptidoglycan					705:717	cell-wall peptidoglycan	695:717	cell-wall peptidoglycan	695:717	Strains MS1-9T and NGC1-4 contained meso-diaminopimelic acid in cell-wall peptidoglycan.
31601450	6	7	dep	Strains	631:637	arg1	NGC1-4					650:655	NGC1-4	650:655	NGC1-4	650:655	Strains MS1-9T and NGC1-4 contained meso-diaminopimelic acid in cell-wall peptidoglycan.
31601450	6	7	dep	Strains	631:637	arg1	MS1-9T					639:644	MS1-9T	639:644	MS1-9T	639:644	Strains MS1-9T and NGC1-4 contained meso-diaminopimelic acid in cell-wall peptidoglycan.
31601450	6	7	dep	Strains	631:637	arg1	Strains					631:637	Strains MS1-9T and NGC1-4	631:655	Strains MS1-9T and NGC1-4	631:655	Strains MS1-9T and NGC1-4 contained meso-diaminopimelic acid in cell-wall peptidoglycan.
31601450	5	8	theme	M.	591:592	arg1	45758T					609:614	M. avicenniae DSM 45758T	591:614	M. avicenniae DSM 45758T	591:614	Comparative analysis of 16S rRNA gene (98.0 to 98.9% similarity), gyrase subunit B (gyrB) gene and whole-genome sequences emphasised that the strains MS1-9T and NGC1-4 showed closely related with Micromonospora peucetia DSM 43363T, M. krabiensis JCM 12869T and M. avicenniae DSM 45758T, respectively.
31601450	5	9	theme	Micromonospora	526:539	arg1	43363T					554:559	Micromonospora peucetia DSM 43363T	526:559	Micromonospora peucetia DSM 43363T	526:559	Comparative analysis of 16S rRNA gene (98.0 to 98.9% similarity), gyrase subunit B (gyrB) gene and whole-genome sequences emphasised that the strains MS1-9T and NGC1-4 showed closely related with Micromonospora peucetia DSM 43363T, M. krabiensis JCM 12869T and M. avicenniae DSM 45758T, respectively.
31601450	5	10	theme	Comparative	330:340	arg1	analysis					342:349	Comparative analysis	330:349	Comparative analysis of 16S rRNA gene (98.0 to 98.9% similarity), gyrase subunit B (gyrB) gene and whole-genome sequences emphasised that the strains MS1-9T and NGC1-4	330:496	Comparative analysis of 16S rRNA gene (98.0 to 98.9% similarity), gyrase subunit B (gyrB) gene and whole-genome sequences emphasised that the strains MS1-9T and NGC1-4 showed closely related with Micromonospora peucetia DSM 43363T, M. krabiensis JCM 12869T and M. avicenniae DSM 45758T, respectively.
31601450	5	11	dep	M.	562:563	arg1	krabiensis					565:574	krabiensis	565:574	krabiensis	565:574	Comparative analysis of 16S rRNA gene (98.0 to 98.9% similarity), gyrase subunit B (gyrB) gene and whole-genome sequences emphasised that the strains MS1-9T and NGC1-4 showed closely related with Micromonospora peucetia DSM 43363T, M. krabiensis JCM 12869T and M. avicenniae DSM 45758T, respectively.
31601450	8	12	theme	peptidoglycan	798:810	arg1	type					790:793	The acyl type	781:793	The acyl type of peptidoglycan	781:810	The acyl type of peptidoglycan was glycolyl.
31601450	8	12	theme	peptidoglycan	798:810	arg1	glycolyl					816:823	glycolyl	816:823	glycolyl	816:823	The acyl type of peptidoglycan was glycolyl.
31601450	13	13	theme	ANI	1281:1283	arg1	values					1285:1290	ANI values	1281:1290	ANI values	1281:1290	Draft genome sequences indicated by ANI values and digital DNA-DNA hybridisation analysis asserted that the strains MS1-9T and NGC1-4 should be represented as a novel species within the genus Micromonospora for which the name Micromonospora musae sp.
31601450	5	14	theme	DSM	605:607	arg1	45758T					609:614	M. avicenniae DSM 45758T	591:614	M. avicenniae DSM 45758T	591:614	Comparative analysis of 16S rRNA gene (98.0 to 98.9% similarity), gyrase subunit B (gyrB) gene and whole-genome sequences emphasised that the strains MS1-9T and NGC1-4 showed closely related with Micromonospora peucetia DSM 43363T, M. krabiensis JCM 12869T and M. avicenniae DSM 45758T, respectively.
31601450	5	15	dep	%	381:381	arg1	98.9					377:380	98.9	377:380	98.9	377:380	Comparative analysis of 16S rRNA gene (98.0 to 98.9% similarity), gyrase subunit B (gyrB) gene and whole-genome sequences emphasised that the strains MS1-9T and NGC1-4 showed closely related with Micromonospora peucetia DSM 43363T, M. krabiensis JCM 12869T and M. avicenniae DSM 45758T, respectively.
31601450	13	16	theme	name	1466:1469	arg1	sp					1492:1493	the name Micromonospora musae sp	1462:1493	the name Micromonospora musae sp	1462:1493	Draft genome sequences indicated by ANI values and digital DNA-DNA hybridisation analysis asserted that the strains MS1-9T and NGC1-4 should be represented as a novel species within the genus Micromonospora for which the name Micromonospora musae sp.
31601450	9	17	theme	major	883:887	arg1	menaquinones					889:900	the major menaquinones	879:900	the major menaquinones	879:900	MK-10(H6), MK-9(H6), and MK-10(H8) were presented as the major menaquinones.
31601450	9	17	theme	major	883:887	arg1	MK-9					837:840	MK-9	837:840	MK-9	837:840	MK-10(H6), MK-9(H6), and MK-10(H8) were presented as the major menaquinones.
31601450	9	17	theme	major	883:887	arg1	MK-10					826:830	MK-10	826:830	MK-10	826:830	MK-10(H6), MK-9(H6), and MK-10(H8) were presented as the major menaquinones.
31601450	9	17	theme	major	883:887	arg1	MK-10					851:855	MK-10	851:855	MK-10	851:855	MK-10(H6), MK-9(H6), and MK-10(H8) were presented as the major menaquinones.
31601450	6	18	from	acid	687:690	arg1	peptidoglycan					705:717	cell-wall peptidoglycan	695:717	cell-wall peptidoglycan	695:717	Strains MS1-9T and NGC1-4 contained meso-diaminopimelic acid in cell-wall peptidoglycan.
31601450	6	19	contain	contained	657:665	arg1	NGC1-4					650:655	NGC1-4	650:655	NGC1-4	650:655	Strains MS1-9T and NGC1-4 contained meso-diaminopimelic acid in cell-wall peptidoglycan.
31601450	6	19	contain	contained	657:665	arg2	acid					687:690	meso-diaminopimelic acid	667:690	meso-diaminopimelic acid in cell-wall peptidoglycan	667:717	Strains MS1-9T and NGC1-4 contained meso-diaminopimelic acid in cell-wall peptidoglycan.
31601450	6	19	contain	contained	657:665	arg1	MS1-9T					639:644	MS1-9T	639:644	MS1-9T	639:644	Strains MS1-9T and NGC1-4 contained meso-diaminopimelic acid in cell-wall peptidoglycan.
31601450	6	19	contain	contained	657:665	arg1	Strains					631:637	Strains MS1-9T and NGC1-4	631:655	Strains MS1-9T and NGC1-4	631:655	Strains MS1-9T and NGC1-4 contained meso-diaminopimelic acid in cell-wall peptidoglycan.
31601450	13	20	theme	Micromonospora	1471:1484	arg1	sp					1492:1493	the name Micromonospora musae sp	1462:1493	the name Micromonospora musae sp	1462:1493	Draft genome sequences indicated by ANI values and digital DNA-DNA hybridisation analysis asserted that the strains MS1-9T and NGC1-4 should be represented as a novel species within the genus Micromonospora for which the name Micromonospora musae sp.
31601450	0	21	theme	musae	15:19	arg1	sp					21:22	Micromonospora musae sp	0:22	Micromonospora musae sp.	0:23	Micromonospora musae sp.
31601450	5	22	theme	whole-genome	429:440	arg1	sequences					442:450	whole-genome sequences	429:450	whole-genome sequences emphasised that the strains MS1-9T and NGC1-4	429:496	Comparative analysis of 16S rRNA gene (98.0 to 98.9% similarity), gyrase subunit B (gyrB) gene and whole-genome sequences emphasised that the strains MS1-9T and NGC1-4 showed closely related with Micromonospora peucetia DSM 43363T, M. krabiensis JCM 12869T and M. avicenniae DSM 45758T, respectively.
31601450	11	23	theme	fatty	1071:1075	arg1	acids					1077:1081	The major cellular fatty acids	1052:1081	The major cellular fatty acids	1052:1081	The major cellular fatty acids consisted of iso-C15:0, anteiso-C15:0, anteiso-C17:0, iso-C17:0 and C17:0.
31601450	12	24	dep	strains	1181:1187	arg1	MS1-9T					1189:1194	MS1-9T	1189:1194	MS1-9T	1189:1194	The DNA G+C content of strains MS1-9T and NGC1-4 were 72.2 and 72.3mol%, respectively.
31601450	12	24	dep	strains	1181:1187	arg1	strains					1181:1187	strains MS1-9T and NGC1-4	1181:1205	strains MS1-9T and NGC1-4	1181:1205	The DNA G+C content of strains MS1-9T and NGC1-4 were 72.2 and 72.3mol%, respectively.
31601450	12	24	dep	strains	1181:1187	arg1	NGC1-4					1200:1205	NGC1-4	1200:1205	NGC1-4	1200:1205	The DNA G+C content of strains MS1-9T and NGC1-4 were 72.2 and 72.3mol%, respectively.
31601450	0	25	theme	Micromonospora	0:13	arg1	sp					21:22	Micromonospora musae sp	0:22	Micromonospora musae sp.	0:23	Micromonospora musae sp.
31601450	1	26	attach	isolated	58:65	arg2	actinomycete					45:56	an endophytic actinomycete	31:56	an endophytic actinomycete isolated from roots of Musa species	31:92	nov., an endophytic actinomycete isolated from roots of Musa species.
31601450	1	26	attach	isolated	58:65	arg1	roots					72:76	roots	72:76	roots of Musa species	72:92	nov., an endophytic actinomycete isolated from roots of Musa species.
31601450	5	27	theme	sequences	442:450	arg1	analysis					342:349	Comparative analysis	330:349	Comparative analysis of 16S rRNA gene (98.0 to 98.9% similarity), gyrase subunit B (gyrB) gene and whole-genome sequences emphasised that the strains MS1-9T and NGC1-4	330:496	Comparative analysis of 16S rRNA gene (98.0 to 98.9% similarity), gyrase subunit B (gyrB) gene and whole-genome sequences emphasised that the strains MS1-9T and NGC1-4 showed closely related with Micromonospora peucetia DSM 43363T, M. krabiensis JCM 12869T and M. avicenniae DSM 45758T, respectively.
31601450	1	28	theme	Musa	81:84	arg1	species					86:92	Musa species	81:92	Musa species	81:92	nov., an endophytic actinomycete isolated from roots of Musa species.
31601450	5	29	theme	gene	363:366	arg1	analysis					342:349	Comparative analysis	330:349	Comparative analysis of 16S rRNA gene (98.0 to 98.9% similarity), gyrase subunit B (gyrB) gene and whole-genome sequences emphasised that the strains MS1-9T and NGC1-4	330:496	Comparative analysis of 16S rRNA gene (98.0 to 98.9% similarity), gyrase subunit B (gyrB) gene and whole-genome sequences emphasised that the strains MS1-9T and NGC1-4 showed closely related with Micromonospora peucetia DSM 43363T, M. krabiensis JCM 12869T and M. avicenniae DSM 45758T, respectively.
31601450	12	30	theme	G+C	1166:1168	arg1	%					1228:1228	72.2 and 72.3mol%	1212:1228	%	1228:1228	The DNA G+C content of strains MS1-9T and NGC1-4 were 72.2 and 72.3mol%, respectively.
31601450	12	30	theme	G+C	1166:1168	arg1	content					1170:1176	The DNA G+C content	1158:1176	The DNA G+C content of strains MS1-9T and NGC1-4	1158:1205	The DNA G+C content of strains MS1-9T and NGC1-4 were 72.2 and 72.3mol%, respectively.
31601450	13	31	theme	Draft	1245:1249	arg1	sequences					1258:1266	Draft genome sequences	1245:1266	Draft genome sequences	1245:1266	Draft genome sequences indicated by ANI values and digital DNA-DNA hybridisation analysis asserted that the strains MS1-9T and NGC1-4 should be represented as a novel species within the genus Micromonospora for which the name Micromonospora musae sp.
31601450	6	32	theme	meso-diaminopimelic	667:685	arg1	acid					687:690	meso-diaminopimelic acid	667:690	meso-diaminopimelic acid in cell-wall peptidoglycan	667:717	Strains MS1-9T and NGC1-4 contained meso-diaminopimelic acid in cell-wall peptidoglycan.
31601450	1	33	theme	species	86:92	arg1	roots					72:76	roots	72:76	roots of Musa species	72:92	nov., an endophytic actinomycete isolated from roots of Musa species.
31601450	15	34	theme	32149T=TISTR	1546:1557	arg1	MS1-9T					1533:1538	MS1-9T	1533:1538	MS1-9T (=JCM 32149T=TISTR 2659T)	1533:1564	The type strain is MS1-9T (=JCM 32149T=TISTR 2659T).
31601450	15	34	theme	32149T=TISTR	1546:1557	arg1	2659T					1559:1563	=JCM 32149T=TISTR 2659T	1541:1563	=JCM 32149T=TISTR 2659T	1541:1563	The type strain is MS1-9T (=JCM 32149T=TISTR 2659T).
31601450	2	35	attach	isolated	154:161	arg2	NGC1-4					141:146	NGC1-4	141:146	NGC1-4	141:146	Two novel actinobacterial strains, MS1-9T and NGC1-4, were isolated from roots of Musa (ABB) cv.
31601450	2	35	attach	isolated	154:161	arg2	MS1-9T					130:135	MS1-9T	130:135	MS1-9T	130:135	Two novel actinobacterial strains, MS1-9T and NGC1-4, were isolated from roots of Musa (ABB) cv.
31601450	2	35	attach	isolated	154:161	arg2	strains					121:127	Two novel actinobacterial strains	95:127	Two novel actinobacterial strains	95:127	Two novel actinobacterial strains, MS1-9T and NGC1-4, were isolated from roots of Musa (ABB) cv.
31601450	2	35	attach	isolated	154:161	arg1	roots					168:172	roots	168:172	roots of Musa (ABB) cv	168:189	Two novel actinobacterial strains, MS1-9T and NGC1-4, were isolated from roots of Musa (ABB) cv.
31601450	12	36	theme	72.3mol	1221:1227	arg1	%					1228:1228	72.2 and 72.3mol%	1212:1228	%	1228:1228	The DNA G+C content of strains MS1-9T and NGC1-4 were 72.2 and 72.3mol%, respectively.
31601450	12	36	theme	72.3mol	1221:1227	arg1	content					1170:1176	The DNA G+C content	1158:1176	The DNA G+C content of strains MS1-9T and NGC1-4	1158:1205	The DNA G+C content of strains MS1-9T and NGC1-4 were 72.2 and 72.3mol%, respectively.
31601450	13	37	theme	genome	1251:1256	arg1	sequences					1258:1266	Draft genome sequences	1245:1266	Draft genome sequences	1245:1266	Draft genome sequences indicated by ANI values and digital DNA-DNA hybridisation analysis asserted that the strains MS1-9T and NGC1-4 should be represented as a novel species within the genus Micromonospora for which the name Micromonospora musae sp.
31601450	5	38	theme	JCM	576:578	arg1	12869T					580:585	M. krabiensis JCM 12869T	562:585	M. krabiensis JCM 12869T	562:585	Comparative analysis of 16S rRNA gene (98.0 to 98.9% similarity), gyrase subunit B (gyrB) gene and whole-genome sequences emphasised that the strains MS1-9T and NGC1-4 showed closely related with Micromonospora peucetia DSM 43363T, M. krabiensis JCM 12869T and M. avicenniae DSM 45758T, respectively.
31601450	11	39	theme	cellular	1062:1069	arg1	acids					1077:1081	The major cellular fatty acids	1052:1081	The major cellular fatty acids	1052:1081	The major cellular fatty acids consisted of iso-C15:0, anteiso-C15:0, anteiso-C17:0, iso-C17:0 and C17:0.
31601450	8	40	theme	acyl	785:788	arg1	type					790:793	The acyl type	781:793	The acyl type of peptidoglycan	781:810	The acyl type of peptidoglycan was glycolyl.
31601450	8	40	theme	acyl	785:788	arg1	glycolyl					816:823	glycolyl	816:823	glycolyl	816:823	The acyl type of peptidoglycan was glycolyl.
31601450	7	41	theme	Whole-cell	720:729	arg1	sugars					731:736	Whole-cell sugars	720:736	Whole-cell sugars	720:736	Whole-cell sugars were glucose, xylose, mannose, and ribose.
31601450	13	42	dep	represented	1389:1399	arg1	asserted					1335:1342	asserted	1335:1342	asserted	1335:1342	Draft genome sequences indicated by ANI values and digital DNA-DNA hybridisation analysis asserted that the strains MS1-9T and NGC1-4 should be represented as a novel species within the genus Micromonospora for which the name Micromonospora musae sp.
31601450	13	43	theme	novel	1406:1410	arg1	species					1412:1418	a novel species	1404:1418	a novel species within the genus Micromonospora for which the name Micromonospora musae sp	1404:1493	Draft genome sequences indicated by ANI values and digital DNA-DNA hybridisation analysis asserted that the strains MS1-9T and NGC1-4 should be represented as a novel species within the genus Micromonospora for which the name Micromonospora musae sp.
31601450	13	43	theme	novel	1406:1410	arg1	strains					1353:1359	the strains MS1-9T and NGC1-4	1349:1377	the strains MS1-9T and NGC1-4	1349:1377	Draft genome sequences indicated by ANI values and digital DNA-DNA hybridisation analysis asserted that the strains MS1-9T and NGC1-4 should be represented as a novel species within the genus Micromonospora for which the name Micromonospora musae sp.
31601450	13	44	dep	Micromonospora	1471:1484	arg1	musae					1486:1490	musae	1486:1490	musae	1486:1490	Draft genome sequences indicated by ANI values and digital DNA-DNA hybridisation analysis asserted that the strains MS1-9T and NGC1-4 should be represented as a novel species within the genus Micromonospora for which the name Micromonospora musae sp.
31601450	12	45	theme	DNA	1162:1164	arg1	%					1228:1228	72.2 and 72.3mol%	1212:1228	%	1228:1228	The DNA G+C content of strains MS1-9T and NGC1-4 were 72.2 and 72.3mol%, respectively.
31601450	12	45	theme	DNA	1162:1164	arg1	content					1170:1176	The DNA G+C content	1158:1176	The DNA G+C content of strains MS1-9T and NGC1-4	1158:1205	The DNA G+C content of strains MS1-9T and NGC1-4 were 72.2 and 72.3mol%, respectively.
31601450	10	46	theme	predominant	1017:1027	arg1	phosphatidylglycerol					953:972	phosphatidylglycerol	953:972	phosphatidylglycerol	953:972	Diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, and phosphatidylinositol were detected as predominant phospholipid profiles.
31601450	10	46	theme	predominant	1017:1027	arg1	phosphatidylinositol					979:998	phosphatidylinositol	979:998	phosphatidylinositol	979:998	Diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, and phosphatidylinositol were detected as predominant phospholipid profiles.
31601450	10	46	theme	predominant	1017:1027	arg1	phosphatidylethanolamine					927:950	phosphatidylethanolamine	927:950	phosphatidylethanolamine	927:950	Diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, and phosphatidylinositol were detected as predominant phospholipid profiles.
31601450	10	46	theme	predominant	1017:1027	arg1	profiles					1042:1049	predominant phospholipid profiles	1017:1049	predominant phospholipid profiles	1017:1049	Diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, and phosphatidylinositol were detected as predominant phospholipid profiles.
31601450	10	46	theme	predominant	1017:1027	arg1	Diphosphatidylglycerol					903:924	Diphosphatidylglycerol	903:924	Diphosphatidylglycerol	903:924	Diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, and phosphatidylinositol were detected as predominant phospholipid profiles.
31601450	15	47	theme	=JCM	1541:1544	arg1	MS1-9T					1533:1538	MS1-9T	1533:1538	MS1-9T (=JCM 32149T=TISTR 2659T)	1533:1564	The type strain is MS1-9T (=JCM 32149T=TISTR 2659T).
31601450	15	47	theme	=JCM	1541:1544	arg1	2659T					1559:1563	=JCM 32149T=TISTR 2659T	1541:1563	=JCM 32149T=TISTR 2659T	1541:1563	The type strain is MS1-9T (=JCM 32149T=TISTR 2659T).
31601450	2	48	theme	cv	188:189	arg1	roots					168:172	roots	168:172	roots of Musa (ABB) cv	168:189	Two novel actinobacterial strains, MS1-9T and NGC1-4, were isolated from roots of Musa (ABB) cv.
31601450	5	49	dep	similarity	383:392	arg1	%					381:381	%	381:381	%	381:381	Comparative analysis of 16S rRNA gene (98.0 to 98.9% similarity), gyrase subunit B (gyrB) gene and whole-genome sequences emphasised that the strains MS1-9T and NGC1-4 showed closely related with Micromonospora peucetia DSM 43363T, M. krabiensis JCM 12869T and M. avicenniae DSM 45758T, respectively.
31601450	5	50	theme	DSM	550:552	arg1	43363T					554:559	Micromonospora peucetia DSM 43363T	526:559	Micromonospora peucetia DSM 43363T	526:559	Comparative analysis of 16S rRNA gene (98.0 to 98.9% similarity), gyrase subunit B (gyrB) gene and whole-genome sequences emphasised that the strains MS1-9T and NGC1-4 showed closely related with Micromonospora peucetia DSM 43363T, M. krabiensis JCM 12869T and M. avicenniae DSM 45758T, respectively.
31601450	13	51	dep	strains	1353:1359	arg1	MS1-9T					1361:1366	MS1-9T	1361:1366	MS1-9T	1361:1366	Draft genome sequences indicated by ANI values and digital DNA-DNA hybridisation analysis asserted that the strains MS1-9T and NGC1-4 should be represented as a novel species within the genus Micromonospora for which the name Micromonospora musae sp.
31601450	13	51	dep	strains	1353:1359	arg1	strains					1353:1359	the strains MS1-9T and NGC1-4	1349:1377	the strains MS1-9T and NGC1-4	1349:1377	Draft genome sequences indicated by ANI values and digital DNA-DNA hybridisation analysis asserted that the strains MS1-9T and NGC1-4 should be represented as a novel species within the genus Micromonospora for which the name Micromonospora musae sp.
31601450	13	51	dep	strains	1353:1359	arg1	NGC1-4					1372:1377	NGC1-4	1372:1377	NGC1-4	1372:1377	Draft genome sequences indicated by ANI values and digital DNA-DNA hybridisation analysis asserted that the strains MS1-9T and NGC1-4 should be represented as a novel species within the genus Micromonospora for which the name Micromonospora musae sp.
31601450	12	52	theme	72.2	1212:1215	arg1	%					1228:1228	72.2 and 72.3mol%	1212:1228	%	1228:1228	The DNA G+C content of strains MS1-9T and NGC1-4 were 72.2 and 72.3mol%, respectively.
31601450	12	52	theme	72.2	1212:1215	arg1	content					1170:1176	The DNA G+C content	1158:1176	The DNA G+C content of strains MS1-9T and NGC1-4	1158:1205	The DNA G+C content of strains MS1-9T and NGC1-4 were 72.2 and 72.3mol%, respectively.
31601450	3	53	theme	Musa	249:252	arg1	cv					260:261	Musa (ABB) cv	249:261	Musa (ABB) cv	249:261	'Kluai Namwa', collected from Chachoengsao province, and Musa (ABB) cv.
31601450	2	54	theme	ABB	183:185	arg1	cv					188:189	Musa (ABB) cv	177:189	Musa (ABB) cv	177:189	Two novel actinobacterial strains, MS1-9T and NGC1-4, were isolated from roots of Musa (ABB) cv.
31601450	5	55	dep	98.9	377:380	arg1	to					374:375	to	374:375	to	374:375	Comparative analysis of 16S rRNA gene (98.0 to 98.9% similarity), gyrase subunit B (gyrB) gene and whole-genome sequences emphasised that the strains MS1-9T and NGC1-4 showed closely related with Micromonospora peucetia DSM 43363T, M. krabiensis JCM 12869T and M. avicenniae DSM 45758T, respectively.
31601450	13	56	theme	digital	1296:1302	arg1	analysis					1326:1333	digital DNA-DNA hybridisation analysis	1296:1333	digital DNA-DNA hybridisation analysis	1296:1333	Draft genome sequences indicated by ANI values and digital DNA-DNA hybridisation analysis asserted that the strains MS1-9T and NGC1-4 should be represented as a novel species within the genus Micromonospora for which the name Micromonospora musae sp.
31601450	5	57	dep	strains	472:478	arg1	strains					472:478	the strains MS1-9T and NGC1-4	468:496	the strains MS1-9T and NGC1-4	468:496	Comparative analysis of 16S rRNA gene (98.0 to 98.9% similarity), gyrase subunit B (gyrB) gene and whole-genome sequences emphasised that the strains MS1-9T and NGC1-4 showed closely related with Micromonospora peucetia DSM 43363T, M. krabiensis JCM 12869T and M. avicenniae DSM 45758T, respectively.
31601450	5	57	dep	strains	472:478	arg1	NGC1-4					491:496	NGC1-4	491:496	NGC1-4	491:496	Comparative analysis of 16S rRNA gene (98.0 to 98.9% similarity), gyrase subunit B (gyrB) gene and whole-genome sequences emphasised that the strains MS1-9T and NGC1-4 showed closely related with Micromonospora peucetia DSM 43363T, M. krabiensis JCM 12869T and M. avicenniae DSM 45758T, respectively.
31601450	5	57	dep	strains	472:478	arg1	MS1-9T					480:485	MS1-9T	480:485	MS1-9T	480:485	Comparative analysis of 16S rRNA gene (98.0 to 98.9% similarity), gyrase subunit B (gyrB) gene and whole-genome sequences emphasised that the strains MS1-9T and NGC1-4 showed closely related with Micromonospora peucetia DSM 43363T, M. krabiensis JCM 12869T and M. avicenniae DSM 45758T, respectively.
31601450	15	58	theme	type	1518:1521	arg1	MS1-9T					1533:1538	MS1-9T	1533:1538	MS1-9T (=JCM 32149T=TISTR 2659T)	1533:1564	The type strain is MS1-9T (=JCM 32149T=TISTR 2659T).
31601450	15	58	theme	type	1518:1521	arg1	strain					1523:1528	The type strain	1514:1528	The type strain	1514:1528	The type strain is MS1-9T (=JCM 32149T=TISTR 2659T).
31601450	5	59	dep	M.	591:592	arg1	avicenniae					594:603	avicenniae	594:603	avicenniae	594:603	Comparative analysis of 16S rRNA gene (98.0 to 98.9% similarity), gyrase subunit B (gyrB) gene and whole-genome sequences emphasised that the strains MS1-9T and NGC1-4 showed closely related with Micromonospora peucetia DSM 43363T, M. krabiensis JCM 12869T and M. avicenniae DSM 45758T, respectively.
31601450	2	60	theme	Musa	177:180	arg1	cv					188:189	Musa (ABB) cv	177:189	Musa (ABB) cv	177:189	Two novel actinobacterial strains, MS1-9T and NGC1-4, were isolated from roots of Musa (ABB) cv.
31601450	12	61	theme	strains	1181:1187	arg1	%					1228:1228	72.2 and 72.3mol%	1212:1228	%	1228:1228	The DNA G+C content of strains MS1-9T and NGC1-4 were 72.2 and 72.3mol%, respectively.
31601450	12	61	theme	strains	1181:1187	arg1	content					1170:1176	The DNA G+C content	1158:1176	The DNA G+C content of strains MS1-9T and NGC1-4	1158:1205	The DNA G+C content of strains MS1-9T and NGC1-4 were 72.2 and 72.3mol%, respectively.
31601450	5	62	theme	gene	420:423	arg1	analysis					342:349	Comparative analysis	330:349	Comparative analysis of 16S rRNA gene (98.0 to 98.9% similarity), gyrase subunit B (gyrB) gene and whole-genome sequences emphasised that the strains MS1-9T and NGC1-4	330:496	Comparative analysis of 16S rRNA gene (98.0 to 98.9% similarity), gyrase subunit B (gyrB) gene and whole-genome sequences emphasised that the strains MS1-9T and NGC1-4 showed closely related with Micromonospora peucetia DSM 43363T, M. krabiensis JCM 12869T and M. avicenniae DSM 45758T, respectively.
31601450	1	63	dep	actinomycete	45:56	arg1	nov.					25:28	nov.	25:28	nov.	25:28	nov., an endophytic actinomycete isolated from roots of Musa species.
31601450	11	64	theme	major	1056:1060	arg1	acids					1077:1081	The major cellular fatty acids	1052:1081	The major cellular fatty acids	1052:1081	The major cellular fatty acids consisted of iso-C15:0, anteiso-C15:0, anteiso-C17:0, iso-C17:0 and C17:0.
31601450	5	65	theme	M.	562:563	arg1	12869T					580:585	M. krabiensis JCM 12869T	562:585	M. krabiensis JCM 12869T	562:585	Comparative analysis of 16S rRNA gene (98.0 to 98.9% similarity), gyrase subunit B (gyrB) gene and whole-genome sequences emphasised that the strains MS1-9T and NGC1-4 showed closely related with Micromonospora peucetia DSM 43363T, M. krabiensis JCM 12869T and M. avicenniae DSM 45758T, respectively.
31601450	13	66	theme	hybridisation	1312:1324	arg1	analysis					1326:1333	digital DNA-DNA hybridisation analysis	1296:1333	digital DNA-DNA hybridisation analysis	1296:1333	Draft genome sequences indicated by ANI values and digital DNA-DNA hybridisation analysis asserted that the strains MS1-9T and NGC1-4 should be represented as a novel species within the genus Micromonospora for which the name Micromonospora musae sp.
31601450	5	67	theme	16S	354:356	arg1	rRNA					358:361	16S rRNA	354:361	16S rRNA gene (98.0 to 98.9% similarity)	354:393	Comparative analysis of 16S rRNA gene (98.0 to 98.9% similarity), gyrase subunit B (gyrB) gene and whole-genome sequences emphasised that the strains MS1-9T and NGC1-4 showed closely related with Micromonospora peucetia DSM 43363T, M. krabiensis JCM 12869T and M. avicenniae DSM 45758T, respectively.
31601450	13	68	theme	DNA-DNA	1304:1310	arg1	analysis					1326:1333	digital DNA-DNA hybridisation analysis	1296:1333	digital DNA-DNA hybridisation analysis	1296:1333	Draft genome sequences indicated by ANI values and digital DNA-DNA hybridisation analysis asserted that the strains MS1-9T and NGC1-4 should be represented as a novel species within the genus Micromonospora for which the name Micromonospora musae sp.
31601450	5	69	theme	gyrase	396:401	arg1	gyrB					414:417	gyrB	414:417	gyrB	414:417	Comparative analysis of 16S rRNA gene (98.0 to 98.9% similarity), gyrase subunit B (gyrB) gene and whole-genome sequences emphasised that the strains MS1-9T and NGC1-4 showed closely related with Micromonospora peucetia DSM 43363T, M. krabiensis JCM 12869T and M. avicenniae DSM 45758T, respectively.
31601450	5	69	theme	gyrase	396:401	arg1	B					411:411	gyrase subunit B	396:411	gyrase subunit B (gyrB) gene	396:423	Comparative analysis of 16S rRNA gene (98.0 to 98.9% similarity), gyrase subunit B (gyrB) gene and whole-genome sequences emphasised that the strains MS1-9T and NGC1-4 showed closely related with Micromonospora peucetia DSM 43363T, M. krabiensis JCM 12869T and M. avicenniae DSM 45758T, respectively.
31601450	5	70	theme	rRNA	358:361	arg1	gene					363:366	16S rRNA gene	354:366	16S rRNA gene (98.0 to 98.9% similarity)	354:393	Comparative analysis of 16S rRNA gene (98.0 to 98.9% similarity), gyrase subunit B (gyrB) gene and whole-genome sequences emphasised that the strains MS1-9T and NGC1-4 showed closely related with Micromonospora peucetia DSM 43363T, M. krabiensis JCM 12869T and M. avicenniae DSM 45758T, respectively.
31601450	5	70	theme	rRNA	358:361	arg1	similarity					383:392	98.0 to 98.9% similarity	369:392	98.0 to 98.9% similarity	369:392	Comparative analysis of 16S rRNA gene (98.0 to 98.9% similarity), gyrase subunit B (gyrB) gene and whole-genome sequences emphasised that the strains MS1-9T and NGC1-4 showed closely related with Micromonospora peucetia DSM 43363T, M. krabiensis JCM 12869T and M. avicenniae DSM 45758T, respectively.
31601450	1	71	theme	endophytic	34:43	arg1	actinomycete					45:56	an endophytic actinomycete	31:56	an endophytic actinomycete isolated from roots of Musa species	31:92	nov., an endophytic actinomycete isolated from roots of Musa species.
31601450	5	72	theme	subunit	403:409	arg1	gyrB					414:417	gyrB	414:417	gyrB	414:417	Comparative analysis of 16S rRNA gene (98.0 to 98.9% similarity), gyrase subunit B (gyrB) gene and whole-genome sequences emphasised that the strains MS1-9T and NGC1-4 showed closely related with Micromonospora peucetia DSM 43363T, M. krabiensis JCM 12869T and M. avicenniae DSM 45758T, respectively.
31601450	5	72	theme	subunit	403:409	arg1	B					411:411	gyrase subunit B	396:411	gyrase subunit B (gyrB) gene	396:423	Comparative analysis of 16S rRNA gene (98.0 to 98.9% similarity), gyrase subunit B (gyrB) gene and whole-genome sequences emphasised that the strains MS1-9T and NGC1-4 showed closely related with Micromonospora peucetia DSM 43363T, M. krabiensis JCM 12869T and M. avicenniae DSM 45758T, respectively.
31601450	5	73	dep	Micromonospora	526:539	arg1	peucetia					541:548	peucetia	541:548	peucetia	541:548	Comparative analysis of 16S rRNA gene (98.0 to 98.9% similarity), gyrase subunit B (gyrB) gene and whole-genome sequences emphasised that the strains MS1-9T and NGC1-4 showed closely related with Micromonospora peucetia DSM 43363T, M. krabiensis JCM 12869T and M. avicenniae DSM 45758T, respectively.
31601450	10	74	theme	phospholipid	1029:1040	arg1	phosphatidylglycerol					953:972	phosphatidylglycerol	953:972	phosphatidylglycerol	953:972	Diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, and phosphatidylinositol were detected as predominant phospholipid profiles.
31601450	10	74	theme	phospholipid	1029:1040	arg1	phosphatidylinositol					979:998	phosphatidylinositol	979:998	phosphatidylinositol	979:998	Diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, and phosphatidylinositol were detected as predominant phospholipid profiles.
31601450	10	74	theme	phospholipid	1029:1040	arg1	phosphatidylethanolamine					927:950	phosphatidylethanolamine	927:950	phosphatidylethanolamine	927:950	Diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, and phosphatidylinositol were detected as predominant phospholipid profiles.
31601450	10	74	theme	phospholipid	1029:1040	arg1	profiles					1042:1049	predominant phospholipid profiles	1017:1049	predominant phospholipid profiles	1017:1049	Diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, and phosphatidylinositol were detected as predominant phospholipid profiles.
31601450	10	74	theme	phospholipid	1029:1040	arg1	Diphosphatidylglycerol					903:924	Diphosphatidylglycerol	903:924	Diphosphatidylglycerol	903:924	Diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, and phosphatidylinositol were detected as predominant phospholipid profiles.
30865978	3	0	from	present	633:639	arg1	membrane					665:672	the milk fat globule membrane	644:672	the milk fat globule membrane	644:672	Human milk proteins can be broadly classified into 3 categories: caseins, whey proteins, and mucins, which are present in the milk fat globule membrane.
30865978	3	1	theme	globule	657:663	arg1	membrane					665:672	the milk fat globule membrane	644:672	the milk fat globule membrane	644:672	Human milk proteins can be broadly classified into 3 categories: caseins, whey proteins, and mucins, which are present in the milk fat globule membrane.
30865978	5	2	theme	significant	820:830	arg1	quantities					832:841	significant quantities	820:841	significant quantities in the whey fraction	820:862	The whey proteins present in significant quantities in the whey fraction are α-lactalbumin, lactoferrin, IgA, osteopontin, and lysozyme.
30865978	4	3	theme	whey/casein	712:722	arg1	ratio					724:728	the whey/casein ratio	708:728	the whey/casein ratio of HM	708:734	HM is whey predominant; however, the whey/casein ratio of HM changes from 90/10 in colostrum to 60/40 in mature HM.
30865978	8	4	theme	current	1360:1366	arg1	evidence					1368:1375	the current evidence	1356:1375	the current evidence on HM protein composition and bioactivity of HM proteins	1356:1432	Herein, the current evidence on HM protein composition and bioactivity of HM proteins is reviewed.
30865978	8	5	theme	proteins	1425:1432	arg1	composition					1391:1401	HM protein composition	1380:1401	HM protein composition	1380:1401	Herein, the current evidence on HM protein composition and bioactivity of HM proteins is reviewed.
30865978	8	5	theme	proteins	1425:1432	arg1	bioactivity					1407:1417	bioactivity	1407:1417	bioactivity of HM proteins	1407:1432	Herein, the current evidence on HM protein composition and bioactivity of HM proteins is reviewed.
30865978	3	6	theme	milk	648:651	arg1	membrane					665:672	the milk fat globule membrane	644:672	the milk fat globule membrane	644:672	Human milk proteins can be broadly classified into 3 categories: caseins, whey proteins, and mucins, which are present in the milk fat globule membrane.
30865978	2	7	dep	shaping	463:469	arg1	microbiome					475:484	the microbiome	471:484	shaping the microbiome	463:484	These functions include serving as a source of amino acids, improving the bioavailability of micronutrients, including vitamins, minerals, and trace elements, providing immunologic defense, stimulating intestinal growth and maturation, shaping the microbiome, and enhancing learning and memory.
30865978	5	8	from	quantities	832:841	arg1	present					809:815	present	809:815	present	809:815	The whey proteins present in significant quantities in the whey fraction are α-lactalbumin, lactoferrin, IgA, osteopontin, and lysozyme.
30865978	5	8	from	quantities	832:841	arg1	fraction					855:862	the whey fraction	846:862	the whey fraction	846:862	The whey proteins present in significant quantities in the whey fraction are α-lactalbumin, lactoferrin, IgA, osteopontin, and lysozyme.
30865978	2	9	theme	amino	274:278	arg1	acids					280:284	amino acids	274:284	amino acids	274:284	These functions include serving as a source of amino acids, improving the bioavailability of micronutrients, including vitamins, minerals, and trace elements, providing immunologic defense, stimulating intestinal growth and maturation, shaping the microbiome, and enhancing learning and memory.
30865978	7	10	theme	available	1322:1330	arg1	formula					1339:1345	commercially available infant formula	1309:1345	commercially available infant formula	1309:1345	Recent advances in dairy technology have enabled isolation of bioactive milk proteins from bovine milk in sufficient quantities for clinical studies and, in some cases, addition to commercially available infant formula.
30865978	7	11	theme	bioactive	1190:1198	arg1	proteins					1205:1212	bioactive milk proteins	1190:1212	bioactive milk proteins from bovine milk	1190:1229	Recent advances in dairy technology have enabled isolation of bioactive milk proteins from bovine milk in sufficient quantities for clinical studies and, in some cases, addition to commercially available infant formula.
30865978	1	12	theme	Human	65:69	arg1	milk					71:74	Human milk	65:74	Human milk (HM)	65:79	Human milk (HM) contains hundreds of proteins with very diverse functions that likely contribute to the short- and long-term beneficial effects of breastfeeding.
30865978	1	12	theme	Human	65:69	arg1	HM					77:78	HM	77:78	HM	77:78	Human milk (HM) contains hundreds of proteins with very diverse functions that likely contribute to the short- and long-term beneficial effects of breastfeeding.
30865978	5	13	from	present	809:815	arg1	quantities					832:841	significant quantities	820:841	significant quantities in the whey fraction	820:862	The whey proteins present in significant quantities in the whey fraction are α-lactalbumin, lactoferrin, IgA, osteopontin, and lysozyme.
30865978	0	14	theme	Human	0:4	arg1	Proteins					11:18	Human Milk Proteins	0:18	Human Milk Proteins	0:18	Human Milk Proteins: Composition and Physiological Significance.
30865978	5	15	attach	present	809:815	arg2	proteins					800:807	The whey proteins	791:807	The whey proteins present in significant quantities in the whey fraction	791:862	The whey proteins present in significant quantities in the whey fraction are α-lactalbumin, lactoferrin, IgA, osteopontin, and lysozyme.
30865978	5	15	attach	present	809:815	arg1	quantities					832:841	significant quantities	820:841	significant quantities in the whey fraction	820:862	The whey proteins present in significant quantities in the whey fraction are α-lactalbumin, lactoferrin, IgA, osteopontin, and lysozyme.
30865978	5	15	attach	present	809:815	arg2	α-lactalbumin					868:880	α-lactalbumin	868:880	α-lactalbumin	868:880	The whey proteins present in significant quantities in the whey fraction are α-lactalbumin, lactoferrin, IgA, osteopontin, and lysozyme.
30865978	2	16	theme	immunologic	396:406	arg1	learning					501:508	learning	501:508	learning	501:508	These functions include serving as a source of amino acids, improving the bioavailability of micronutrients, including vitamins, minerals, and trace elements, providing immunologic defense, stimulating intestinal growth and maturation, shaping the microbiome, and enhancing learning and memory.
30865978	2	16	theme	immunologic	396:406	arg1	memory					514:519	memory	514:519	memory	514:519	These functions include serving as a source of amino acids, improving the bioavailability of micronutrients, including vitamins, minerals, and trace elements, providing immunologic defense, stimulating intestinal growth and maturation, shaping the microbiome, and enhancing learning and memory.
30865978	2	16	theme	immunologic	396:406	arg1	growth					440:445	intestinal growth	429:445	intestinal growth	429:445	These functions include serving as a source of amino acids, improving the bioavailability of micronutrients, including vitamins, minerals, and trace elements, providing immunologic defense, stimulating intestinal growth and maturation, shaping the microbiome, and enhancing learning and memory.
30865978	2	16	theme	immunologic	396:406	arg1	source					264:269	a source	262:269	a source of amino acids	262:284	These functions include serving as a source of amino acids, improving the bioavailability of micronutrients, including vitamins, minerals, and trace elements, providing immunologic defense, stimulating intestinal growth and maturation, shaping the microbiome, and enhancing learning and memory.
30865978	2	16	theme	immunologic	396:406	arg1	bioavailability					301:315	the bioavailability	297:315	the bioavailability of micronutrients, including vitamins, minerals, and trace elements	297:383	These functions include serving as a source of amino acids, improving the bioavailability of micronutrients, including vitamins, minerals, and trace elements, providing immunologic defense, stimulating intestinal growth and maturation, shaping the microbiome, and enhancing learning and memory.
30865978	2	16	theme	immunologic	396:406	arg1	defense					408:414	immunologic defense	396:414	immunologic defense	396:414	These functions include serving as a source of amino acids, improving the bioavailability of micronutrients, including vitamins, minerals, and trace elements, providing immunologic defense, stimulating intestinal growth and maturation, shaping the microbiome, and enhancing learning and memory.
30865978	2	16	theme	immunologic	396:406	arg1	maturation					451:460	maturation	451:460	maturation	451:460	These functions include serving as a source of amino acids, improving the bioavailability of micronutrients, including vitamins, minerals, and trace elements, providing immunologic defense, stimulating intestinal growth and maturation, shaping the microbiome, and enhancing learning and memory.
30865978	6	17	theme	functional	1090:1099	arg1	properties					1101:1110	the functional properties	1086:1110	the functional properties of HM proteins	1086:1125	Additionally, bioactive peptides are formed during digestion of casein and whey, and glycans from glycoproteins are bifidogenic, adding further complexity to the functional properties of HM proteins.
30865978	6	18	theme	casein	992:997	arg1	digestion					979:987	digestion	979:987	digestion of casein and whey	979:1006	Additionally, bioactive peptides are formed during digestion of casein and whey, and glycans from glycoproteins are bifidogenic, adding further complexity to the functional properties of HM proteins.
30865978	6	19	theme	whey	1003:1006	arg1	digestion					979:987	digestion	979:987	digestion of casein and whey	979:1006	Additionally, bioactive peptides are formed during digestion of casein and whey, and glycans from glycoproteins are bifidogenic, adding further complexity to the functional properties of HM proteins.
30865978	7	20	theme	sufficient	1234:1243	arg1	quantities					1245:1254	sufficient quantities	1234:1254	sufficient quantities for clinical studies	1234:1275	Recent advances in dairy technology have enabled isolation of bioactive milk proteins from bovine milk in sufficient quantities for clinical studies and, in some cases, addition to commercially available infant formula.
30865978	3	21	attach	present	633:639	arg2	proteins					601:608	whey proteins	596:608	whey proteins	596:608	Human milk proteins can be broadly classified into 3 categories: caseins, whey proteins, and mucins, which are present in the milk fat globule membrane.
30865978	3	21	attach	present	633:639	arg2	categories					575:584	3 categories	573:584	3 categories	573:584	Human milk proteins can be broadly classified into 3 categories: caseins, whey proteins, and mucins, which are present in the milk fat globule membrane.
30865978	3	21	attach	present	633:639	arg2	caseins					587:593	caseins	587:593	caseins	587:593	Human milk proteins can be broadly classified into 3 categories: caseins, whey proteins, and mucins, which are present in the milk fat globule membrane.
30865978	3	21	attach	present	633:639	arg1	membrane					665:672	the milk fat globule membrane	644:672	the milk fat globule membrane	644:672	Human milk proteins can be broadly classified into 3 categories: caseins, whey proteins, and mucins, which are present in the milk fat globule membrane.
30865978	3	21	attach	present	633:639	arg2	mucins					615:620	mucins	615:620	mucins	615:620	Human milk proteins can be broadly classified into 3 categories: caseins, whey proteins, and mucins, which are present in the milk fat globule membrane.
30865978	8	22	theme	protein	1383:1389	arg1	composition					1391:1401	HM protein composition	1380:1401	HM protein composition	1380:1401	Herein, the current evidence on HM protein composition and bioactivity of HM proteins is reviewed.
30865978	2	23	theme	trace	370:374	arg1	elements					376:383	trace elements	370:383	trace elements	370:383	These functions include serving as a source of amino acids, improving the bioavailability of micronutrients, including vitamins, minerals, and trace elements, providing immunologic defense, stimulating intestinal growth and maturation, shaping the microbiome, and enhancing learning and memory.
30865978	0	24	theme	Milk	6:9	arg1	Proteins					11:18	Human Milk Proteins	0:18	Human Milk Proteins	0:18	Human Milk Proteins: Composition and Physiological Significance.
30865978	4	25	from	60/40	771:775	arg1	HM					787:788	mature HM	780:788	mature HM	780:788	HM is whey predominant; however, the whey/casein ratio of HM changes from 90/10 in colostrum to 60/40 in mature HM.
30865978	6	26	gly	glycoproteins	1026:1038	arg1	glycoproteins					1026:1038	glycoproteins	1026:1038	glycoproteins	1026:1038	Additionally, bioactive peptides are formed during digestion of casein and whey, and glycans from glycoproteins are bifidogenic, adding further complexity to the functional properties of HM proteins.
30865978	7	27	theme	infant	1332:1337	arg1	formula					1339:1345	commercially available infant formula	1309:1345	commercially available infant formula	1309:1345	Recent advances in dairy technology have enabled isolation of bioactive milk proteins from bovine milk in sufficient quantities for clinical studies and, in some cases, addition to commercially available infant formula.
30865978	5	28	theme	whey	795:798	arg1	α-lactalbumin					868:880	α-lactalbumin	868:880	α-lactalbumin	868:880	The whey proteins present in significant quantities in the whey fraction are α-lactalbumin, lactoferrin, IgA, osteopontin, and lysozyme.
30865978	5	28	theme	whey	795:798	arg1	proteins					800:807	The whey proteins	791:807	The whey proteins present in significant quantities in the whey fraction	791:862	The whey proteins present in significant quantities in the whey fraction are α-lactalbumin, lactoferrin, IgA, osteopontin, and lysozyme.
30865978	3	29	located	present	633:639	arg2	proteins					601:608	whey proteins	596:608	whey proteins	596:608	Human milk proteins can be broadly classified into 3 categories: caseins, whey proteins, and mucins, which are present in the milk fat globule membrane.
30865978	3	29	located	present	633:639	arg2	categories					575:584	3 categories	573:584	3 categories	573:584	Human milk proteins can be broadly classified into 3 categories: caseins, whey proteins, and mucins, which are present in the milk fat globule membrane.
30865978	3	29	located	present	633:639	arg2	caseins					587:593	caseins	587:593	caseins	587:593	Human milk proteins can be broadly classified into 3 categories: caseins, whey proteins, and mucins, which are present in the milk fat globule membrane.
30865978	3	29	located	present	633:639	arg1	membrane					665:672	the milk fat globule membrane	644:672	the milk fat globule membrane	644:672	Human milk proteins can be broadly classified into 3 categories: caseins, whey proteins, and mucins, which are present in the milk fat globule membrane.
30865978	3	29	located	present	633:639	arg2	mucins					615:620	mucins	615:620	mucins	615:620	Human milk proteins can be broadly classified into 3 categories: caseins, whey proteins, and mucins, which are present in the milk fat globule membrane.
30865978	1	30	with	proteins	102:109	arg1	functions					129:137	very diverse functions	116:137	very diverse functions that likely contribute to the short- and long-term beneficial effects of breastfeeding	116:224	Human milk (HM) contains hundreds of proteins with very diverse functions that likely contribute to the short- and long-term beneficial effects of breastfeeding.
30865978	7	31	from	advances	1135:1142	arg1	technology					1153:1162	dairy technology	1147:1162	dairy technology	1147:1162	Recent advances in dairy technology have enabled isolation of bioactive milk proteins from bovine milk in sufficient quantities for clinical studies and, in some cases, addition to commercially available infant formula.
30865978	1	32	theme	short-	169:174	arg1	effects					201:207	the short- and long-term beneficial effects	165:207	the short- and long-term beneficial effects of breastfeeding	165:224	Human milk (HM) contains hundreds of proteins with very diverse functions that likely contribute to the short- and long-term beneficial effects of breastfeeding.
30865978	8	33	from	evidence	1368:1375	arg1	composition					1391:1401	HM protein composition	1380:1401	HM protein composition	1380:1401	Herein, the current evidence on HM protein composition and bioactivity of HM proteins is reviewed.
30865978	8	33	from	evidence	1368:1375	arg1	bioactivity					1407:1417	bioactivity	1407:1417	bioactivity of HM proteins	1407:1432	Herein, the current evidence on HM protein composition and bioactivity of HM proteins is reviewed.
30865978	6	34	theme	further	1064:1070	arg1	complexity					1072:1081	further complexity	1064:1081	further complexity	1064:1081	Additionally, bioactive peptides are formed during digestion of casein and whey, and glycans from glycoproteins are bifidogenic, adding further complexity to the functional properties of HM proteins.
30865978	4	35	dep	60/40	771:775	arg1	colostrum					758:766	colostrum	758:766	colostrum	758:766	HM is whey predominant; however, the whey/casein ratio of HM changes from 90/10 in colostrum to 60/40 in mature HM.
30865978	2	36	theme	intestinal	429:438	arg1	learning					501:508	learning	501:508	learning	501:508	These functions include serving as a source of amino acids, improving the bioavailability of micronutrients, including vitamins, minerals, and trace elements, providing immunologic defense, stimulating intestinal growth and maturation, shaping the microbiome, and enhancing learning and memory.
30865978	2	36	theme	intestinal	429:438	arg1	memory					514:519	memory	514:519	memory	514:519	These functions include serving as a source of amino acids, improving the bioavailability of micronutrients, including vitamins, minerals, and trace elements, providing immunologic defense, stimulating intestinal growth and maturation, shaping the microbiome, and enhancing learning and memory.
30865978	2	36	theme	intestinal	429:438	arg1	growth					440:445	intestinal growth	429:445	intestinal growth	429:445	These functions include serving as a source of amino acids, improving the bioavailability of micronutrients, including vitamins, minerals, and trace elements, providing immunologic defense, stimulating intestinal growth and maturation, shaping the microbiome, and enhancing learning and memory.
30865978	2	36	theme	intestinal	429:438	arg1	source					264:269	a source	262:269	a source of amino acids	262:284	These functions include serving as a source of amino acids, improving the bioavailability of micronutrients, including vitamins, minerals, and trace elements, providing immunologic defense, stimulating intestinal growth and maturation, shaping the microbiome, and enhancing learning and memory.
30865978	2	36	theme	intestinal	429:438	arg1	bioavailability					301:315	the bioavailability	297:315	the bioavailability of micronutrients, including vitamins, minerals, and trace elements	297:383	These functions include serving as a source of amino acids, improving the bioavailability of micronutrients, including vitamins, minerals, and trace elements, providing immunologic defense, stimulating intestinal growth and maturation, shaping the microbiome, and enhancing learning and memory.
30865978	2	36	theme	intestinal	429:438	arg1	defense					408:414	immunologic defense	396:414	immunologic defense	396:414	These functions include serving as a source of amino acids, improving the bioavailability of micronutrients, including vitamins, minerals, and trace elements, providing immunologic defense, stimulating intestinal growth and maturation, shaping the microbiome, and enhancing learning and memory.
30865978	2	36	theme	intestinal	429:438	arg1	maturation					451:460	maturation	451:460	maturation	451:460	These functions include serving as a source of amino acids, improving the bioavailability of micronutrients, including vitamins, minerals, and trace elements, providing immunologic defense, stimulating intestinal growth and maturation, shaping the microbiome, and enhancing learning and memory.
30865978	2	36	theme	intestinal	429:438	arg1	shaping					463:469	shaping the microbiome	463:484	shaping the microbiome	463:484	These functions include serving as a source of amino acids, improving the bioavailability of micronutrients, including vitamins, minerals, and trace elements, providing immunologic defense, stimulating intestinal growth and maturation, shaping the microbiome, and enhancing learning and memory.
30865978	7	37	from	milk	1226:1229	arg1	isolation					1177:1185	isolation	1177:1185	isolation of bioactive milk proteins from bovine milk	1177:1229	Recent advances in dairy technology have enabled isolation of bioactive milk proteins from bovine milk in sufficient quantities for clinical studies and, in some cases, addition to commercially available infant formula.
30865978	7	37	from	milk	1226:1229	arg1	proteins					1205:1212	bioactive milk proteins	1190:1212	bioactive milk proteins from bovine milk	1190:1229	Recent advances in dairy technology have enabled isolation of bioactive milk proteins from bovine milk in sufficient quantities for clinical studies and, in some cases, addition to commercially available infant formula.
30865978	2	38	theme	acids	280:284	arg1	learning					501:508	learning	501:508	learning	501:508	These functions include serving as a source of amino acids, improving the bioavailability of micronutrients, including vitamins, minerals, and trace elements, providing immunologic defense, stimulating intestinal growth and maturation, shaping the microbiome, and enhancing learning and memory.
30865978	2	38	theme	acids	280:284	arg1	memory					514:519	memory	514:519	memory	514:519	These functions include serving as a source of amino acids, improving the bioavailability of micronutrients, including vitamins, minerals, and trace elements, providing immunologic defense, stimulating intestinal growth and maturation, shaping the microbiome, and enhancing learning and memory.
30865978	2	38	theme	acids	280:284	arg1	growth					440:445	intestinal growth	429:445	intestinal growth	429:445	These functions include serving as a source of amino acids, improving the bioavailability of micronutrients, including vitamins, minerals, and trace elements, providing immunologic defense, stimulating intestinal growth and maturation, shaping the microbiome, and enhancing learning and memory.
30865978	2	38	theme	acids	280:284	arg1	source					264:269	a source	262:269	a source of amino acids	262:284	These functions include serving as a source of amino acids, improving the bioavailability of micronutrients, including vitamins, minerals, and trace elements, providing immunologic defense, stimulating intestinal growth and maturation, shaping the microbiome, and enhancing learning and memory.
30865978	2	38	theme	acids	280:284	arg1	bioavailability					301:315	the bioavailability	297:315	the bioavailability of micronutrients, including vitamins, minerals, and trace elements	297:383	These functions include serving as a source of amino acids, improving the bioavailability of micronutrients, including vitamins, minerals, and trace elements, providing immunologic defense, stimulating intestinal growth and maturation, shaping the microbiome, and enhancing learning and memory.
30865978	2	38	theme	acids	280:284	arg1	defense					408:414	immunologic defense	396:414	immunologic defense	396:414	These functions include serving as a source of amino acids, improving the bioavailability of micronutrients, including vitamins, minerals, and trace elements, providing immunologic defense, stimulating intestinal growth and maturation, shaping the microbiome, and enhancing learning and memory.
30865978	2	38	theme	acids	280:284	arg1	maturation					451:460	maturation	451:460	maturation	451:460	These functions include serving as a source of amino acids, improving the bioavailability of micronutrients, including vitamins, minerals, and trace elements, providing immunologic defense, stimulating intestinal growth and maturation, shaping the microbiome, and enhancing learning and memory.
30865978	5	39	theme	present	809:815	arg1	α-lactalbumin					868:880	α-lactalbumin	868:880	α-lactalbumin	868:880	The whey proteins present in significant quantities in the whey fraction are α-lactalbumin, lactoferrin, IgA, osteopontin, and lysozyme.
30865978	5	39	theme	present	809:815	arg1	proteins					800:807	The whey proteins	791:807	The whey proteins present in significant quantities in the whey fraction	791:862	The whey proteins present in significant quantities in the whey fraction are α-lactalbumin, lactoferrin, IgA, osteopontin, and lysozyme.
30865978	1	40	theme	long-term	180:188	arg1	effects					201:207	the short- and long-term beneficial effects	165:207	the short- and long-term beneficial effects of breastfeeding	165:224	Human milk (HM) contains hundreds of proteins with very diverse functions that likely contribute to the short- and long-term beneficial effects of breastfeeding.
30865978	2	41	theme	micronutrients	320:333	arg1	learning					501:508	learning	501:508	learning	501:508	These functions include serving as a source of amino acids, improving the bioavailability of micronutrients, including vitamins, minerals, and trace elements, providing immunologic defense, stimulating intestinal growth and maturation, shaping the microbiome, and enhancing learning and memory.
30865978	2	41	theme	micronutrients	320:333	arg1	memory					514:519	memory	514:519	memory	514:519	These functions include serving as a source of amino acids, improving the bioavailability of micronutrients, including vitamins, minerals, and trace elements, providing immunologic defense, stimulating intestinal growth and maturation, shaping the microbiome, and enhancing learning and memory.
30865978	2	41	theme	micronutrients	320:333	arg1	growth					440:445	intestinal growth	429:445	intestinal growth	429:445	These functions include serving as a source of amino acids, improving the bioavailability of micronutrients, including vitamins, minerals, and trace elements, providing immunologic defense, stimulating intestinal growth and maturation, shaping the microbiome, and enhancing learning and memory.
30865978	2	41	theme	micronutrients	320:333	arg1	source					264:269	a source	262:269	a source of amino acids	262:284	These functions include serving as a source of amino acids, improving the bioavailability of micronutrients, including vitamins, minerals, and trace elements, providing immunologic defense, stimulating intestinal growth and maturation, shaping the microbiome, and enhancing learning and memory.
30865978	2	41	theme	micronutrients	320:333	arg1	bioavailability					301:315	the bioavailability	297:315	the bioavailability of micronutrients, including vitamins, minerals, and trace elements	297:383	These functions include serving as a source of amino acids, improving the bioavailability of micronutrients, including vitamins, minerals, and trace elements, providing immunologic defense, stimulating intestinal growth and maturation, shaping the microbiome, and enhancing learning and memory.
30865978	2	41	theme	micronutrients	320:333	arg1	defense					408:414	immunologic defense	396:414	immunologic defense	396:414	These functions include serving as a source of amino acids, improving the bioavailability of micronutrients, including vitamins, minerals, and trace elements, providing immunologic defense, stimulating intestinal growth and maturation, shaping the microbiome, and enhancing learning and memory.
30865978	2	41	theme	micronutrients	320:333	arg1	maturation					451:460	maturation	451:460	maturation	451:460	These functions include serving as a source of amino acids, improving the bioavailability of micronutrients, including vitamins, minerals, and trace elements, providing immunologic defense, stimulating intestinal growth and maturation, shaping the microbiome, and enhancing learning and memory.
30865978	7	42	theme	bovine	1219:1224	arg1	milk					1226:1229	bovine milk	1219:1229	bovine milk	1219:1229	Recent advances in dairy technology have enabled isolation of bioactive milk proteins from bovine milk in sufficient quantities for clinical studies and, in some cases, addition to commercially available infant formula.
30865978	1	43	theme	beneficial	190:199	arg1	effects					201:207	the short- and long-term beneficial effects	165:207	the short- and long-term beneficial effects of breastfeeding	165:224	Human milk (HM) contains hundreds of proteins with very diverse functions that likely contribute to the short- and long-term beneficial effects of breastfeeding.
30865978	7	44	theme	clinical	1260:1267	arg1	studies					1269:1275	clinical studies	1260:1275	clinical studies	1260:1275	Recent advances in dairy technology have enabled isolation of bioactive milk proteins from bovine milk in sufficient quantities for clinical studies and, in some cases, addition to commercially available infant formula.
30865978	8	45	theme	HM	1380:1381	arg1	composition					1391:1401	HM protein composition	1380:1401	HM protein composition	1380:1401	Herein, the current evidence on HM protein composition and bioactivity of HM proteins is reviewed.
30865978	1	46	contain	contains	81:88	arg1	milk					71:74	Human milk	65:74	Human milk (HM)	65:79	Human milk (HM) contains hundreds of proteins with very diverse functions that likely contribute to the short- and long-term beneficial effects of breastfeeding.
30865978	1	46	contain	contains	81:88	arg2	hundreds					90:97	hundreds	90:97	hundreds of proteins with very diverse functions that likely contribute to the short- and long-term beneficial effects of breastfeeding	90:224	Human milk (HM) contains hundreds of proteins with very diverse functions that likely contribute to the short- and long-term beneficial effects of breastfeeding.
30865978	1	46	contain	contains	81:88	arg1	HM					77:78	HM	77:78	HM	77:78	Human milk (HM) contains hundreds of proteins with very diverse functions that likely contribute to the short- and long-term beneficial effects of breastfeeding.
30865978	4	47	theme	mature	780:785	arg1	HM					787:788	mature HM	780:788	mature HM	780:788	HM is whey predominant; however, the whey/casein ratio of HM changes from 90/10 in colostrum to 60/40 in mature HM.
30865978	7	48	theme	proteins	1205:1212	arg1	isolation					1177:1185	isolation	1177:1185	isolation of bioactive milk proteins from bovine milk	1177:1229	Recent advances in dairy technology have enabled isolation of bioactive milk proteins from bovine milk in sufficient quantities for clinical studies and, in some cases, addition to commercially available infant formula.
30865978	0	49	theme	Physiological	37:49	arg1	Significance					51:62	Physiological Significance	37:62	Physiological Significance	37:62	Human Milk Proteins: Composition and Physiological Significance.
30865978	8	50	theme	HM	1422:1423	arg1	proteins					1425:1432	HM proteins	1422:1432	HM proteins	1422:1432	Herein, the current evidence on HM protein composition and bioactivity of HM proteins is reviewed.
30865978	7	51	from	isolation	1177:1185	arg1	milk					1226:1229	bovine milk	1219:1229	bovine milk	1219:1229	Recent advances in dairy technology have enabled isolation of bioactive milk proteins from bovine milk in sufficient quantities for clinical studies and, in some cases, addition to commercially available infant formula.
30865978	0	52	dep	Composition	21:31	arg1	Proteins					11:18	Human Milk Proteins	0:18	Human Milk Proteins	0:18	Human Milk Proteins: Composition and Physiological Significance.
30865978	1	53	theme	proteins	102:109	arg1	hundreds					90:97	hundreds	90:97	hundreds of proteins with very diverse functions that likely contribute to the short- and long-term beneficial effects of breastfeeding	90:224	Human milk (HM) contains hundreds of proteins with very diverse functions that likely contribute to the short- and long-term beneficial effects of breastfeeding.
30865978	6	54	from	glycoproteins	1026:1038	arg1	glycans					1013:1019	glycans	1013:1019	glycans from glycoproteins	1013:1038	Additionally, bioactive peptides are formed during digestion of casein and whey, and glycans from glycoproteins are bifidogenic, adding further complexity to the functional properties of HM proteins.
30865978	4	55	theme	HM	733:734	arg1	ratio					724:728	the whey/casein ratio	708:728	the whey/casein ratio of HM	708:734	HM is whey predominant; however, the whey/casein ratio of HM changes from 90/10 in colostrum to 60/40 in mature HM.
30865978	3	56	theme	whey	596:599	arg1	proteins					601:608	whey proteins	596:608	whey proteins	596:608	Human milk proteins can be broadly classified into 3 categories: caseins, whey proteins, and mucins, which are present in the milk fat globule membrane.
30865978	6	57	theme	bioactive	942:950	arg1	peptides					952:959	bioactive peptides	942:959	bioactive peptides	942:959	Additionally, bioactive peptides are formed during digestion of casein and whey, and glycans from glycoproteins are bifidogenic, adding further complexity to the functional properties of HM proteins.
30865978	5	58	theme	whey	850:853	arg1	fraction					855:862	the whey fraction	846:862	the whey fraction	846:862	The whey proteins present in significant quantities in the whey fraction are α-lactalbumin, lactoferrin, IgA, osteopontin, and lysozyme.
30865978	3	59	theme	Human	522:526	arg1	proteins					533:540	Human milk proteins	522:540	Human milk proteins	522:540	Human milk proteins can be broadly classified into 3 categories: caseins, whey proteins, and mucins, which are present in the milk fat globule membrane.
30865978	3	60	theme	milk	528:531	arg1	proteins					533:540	Human milk proteins	522:540	Human milk proteins	522:540	Human milk proteins can be broadly classified into 3 categories: caseins, whey proteins, and mucins, which are present in the milk fat globule membrane.
30865978	7	61	theme	milk	1200:1203	arg1	proteins					1205:1212	bioactive milk proteins	1190:1212	bioactive milk proteins from bovine milk	1190:1229	Recent advances in dairy technology have enabled isolation of bioactive milk proteins from bovine milk in sufficient quantities for clinical studies and, in some cases, addition to commercially available infant formula.
30865978	1	62	theme	breastfeeding	212:224	arg1	effects					201:207	the short- and long-term beneficial effects	165:207	the short- and long-term beneficial effects of breastfeeding	165:224	Human milk (HM) contains hundreds of proteins with very diverse functions that likely contribute to the short- and long-term beneficial effects of breastfeeding.
30865978	7	63	theme	dairy	1147:1151	arg1	technology					1153:1162	dairy technology	1147:1162	dairy technology	1147:1162	Recent advances in dairy technology have enabled isolation of bioactive milk proteins from bovine milk in sufficient quantities for clinical studies and, in some cases, addition to commercially available infant formula.
30865978	6	64	theme	proteins	1118:1125	arg1	properties					1101:1110	the functional properties	1086:1110	the functional properties of HM proteins	1086:1125	Additionally, bioactive peptides are formed during digestion of casein and whey, and glycans from glycoproteins are bifidogenic, adding further complexity to the functional properties of HM proteins.
30865978	1	65	theme	diverse	121:127	arg1	functions					129:137	very diverse functions	116:137	very diverse functions that likely contribute to the short- and long-term beneficial effects of breastfeeding	116:224	Human milk (HM) contains hundreds of proteins with very diverse functions that likely contribute to the short- and long-term beneficial effects of breastfeeding.
30865978	3	66	theme	fat	653:655	arg1	membrane					665:672	the milk fat globule membrane	644:672	the milk fat globule membrane	644:672	Human milk proteins can be broadly classified into 3 categories: caseins, whey proteins, and mucins, which are present in the milk fat globule membrane.
30865978	6	67	theme	HM	1115:1116	arg1	proteins					1118:1125	HM proteins	1115:1125	HM proteins	1115:1125	Additionally, bioactive peptides are formed during digestion of casein and whey, and glycans from glycoproteins are bifidogenic, adding further complexity to the functional properties of HM proteins.
30865978	3	68	from	membrane	665:672	arg1	present					633:639	present	633:639	present	633:639	Human milk proteins can be broadly classified into 3 categories: caseins, whey proteins, and mucins, which are present in the milk fat globule membrane.
30865978	7	69	theme	Recent	1128:1133	arg1	advances					1135:1142	Recent advances	1128:1142	Recent advances in dairy technology	1128:1162	Recent advances in dairy technology have enabled isolation of bioactive milk proteins from bovine milk in sufficient quantities for clinical studies and, in some cases, addition to commercially available infant formula.
29896535	1	0	theme	soluble	237:243	arg1	polysaccharides					245:259	the water soluble polysaccharides	227:259	the water soluble polysaccharides from Zizyphus lotus fruit	227:285	Gas chromatography coupled to mass spectrometer (GC-MS) was used to identify and to quantify neutral sugars that constitute the water soluble polysaccharides from Zizyphus lotus fruit.
29896535	0	1	theme	Zizyphus	81:88	arg1	fruit					96:100	Zizyphus lotus fruit	81:100	Zizyphus lotus fruit	81:100	GC-EI-MS identification data of neutral sugars of polysaccharides extracted from Zizyphus lotus fruit.
29896535	5	2	dep	"	832:832	arg1	Hammi					844:848	Hammi	844:848	Hammi	844:848	Data is related to "Optimization extraction of polysaccharide from Tunisian Zizyphus lotus fruit by response surface methodology: Composition and antioxidant activity" (Mkadmini Hammi et al., 2016) [1].
29896535	5	3	theme	polysaccharide	713:726	arg1	extraction					699:708	Optimization extraction	686:708	"Optimization extraction of polysaccharide from Tunisian Zizyphus lotus fruit by response surface methodology: Composition and antioxidant activity" (Mkadmini Hammi et al., 2016) [1]	685:866	Data is related to "Optimization extraction of polysaccharide from Tunisian Zizyphus lotus fruit by response surface methodology: Composition and antioxidant activity" (Mkadmini Hammi et al., 2016) [1].
29896535	3	4	theme	Spectral	563:570	arg1	Library					572:578	the NIST MS Spectral Library	551:578	the NIST MS Spectral Library	551:578	Sugars were identified based on their retention times compared with those of standards and the NIST MS Spectral Library.
29896535	3	5	theme	NIST	555:558	arg1	Library					572:578	the NIST MS Spectral Library	551:578	the NIST MS Spectral Library	551:578	Sugars were identified based on their retention times compared with those of standards and the NIST MS Spectral Library.
29896535	1	6	theme	Zizyphus	266:273	arg1	fruit					281:285	Zizyphus lotus fruit	266:285	Zizyphus lotus fruit	266:285	Gas chromatography coupled to mass spectrometer (GC-MS) was used to identify and to quantify neutral sugars that constitute the water soluble polysaccharides from Zizyphus lotus fruit.
29896535	5	7	theme	Tunisian	733:740	arg1	fruit					757:761	Tunisian Zizyphus lotus fruit	733:761	Tunisian Zizyphus lotus fruit	733:761	Data is related to "Optimization extraction of polysaccharide from Tunisian Zizyphus lotus fruit by response surface methodology: Composition and antioxidant activity" (Mkadmini Hammi et al., 2016) [1].
29896535	2	8	theme	extracted	393:401	arg1	polysaccharides					403:417	extracted polysaccharides	393:417	extracted polysaccharides that were released by hydrolysis method	393:457	The trimethylsilyl (TMS) method was successfully used for derivatization of the monosaccharides units of extracted polysaccharides that were released by hydrolysis method.
29896535	5	9	theme	Zizyphus	742:749	arg1	fruit					757:761	Tunisian Zizyphus lotus fruit	733:761	Tunisian Zizyphus lotus fruit	733:761	Data is related to "Optimization extraction of polysaccharide from Tunisian Zizyphus lotus fruit by response surface methodology: Composition and antioxidant activity" (Mkadmini Hammi et al., 2016) [1].
29896535	5	10	theme	surface	775:781	arg1	methodology					783:793	response surface methodology	766:793	response surface methodology	766:793	Data is related to "Optimization extraction of polysaccharide from Tunisian Zizyphus lotus fruit by response surface methodology: Composition and antioxidant activity" (Mkadmini Hammi et al., 2016) [1].
29896535	5	11	theme	antioxidant	812:822	arg1	activity					824:831	antioxidant activity	812:831	antioxidant activity	812:831	Data is related to "Optimization extraction of polysaccharide from Tunisian Zizyphus lotus fruit by response surface methodology: Composition and antioxidant activity" (Mkadmini Hammi et al., 2016) [1].
29896535	0	12	theme	identification	9:22	arg1	data					24:27	GC-EI-MS identification data	0:27	GC-EI-MS identification data of neutral sugars of polysaccharides	0:64	GC-EI-MS identification data of neutral sugars of polysaccharides extracted from Zizyphus lotus fruit.
29896535	5	13	theme	response	766:773	arg1	methodology					783:793	response surface methodology	766:793	response surface methodology	766:793	Data is related to "Optimization extraction of polysaccharide from Tunisian Zizyphus lotus fruit by response surface methodology: Composition and antioxidant activity" (Mkadmini Hammi et al., 2016) [1].
29896535	2	14	theme	units	384:388	arg1	derivatization					346:359	derivatization	346:359	derivatization of the monosaccharides units of extracted polysaccharides that were released by hydrolysis method	346:457	The trimethylsilyl (TMS) method was successfully used for derivatization of the monosaccharides units of extracted polysaccharides that were released by hydrolysis method.
29896535	0	15	theme	GC-EI-MS	0:7	arg1	data					24:27	GC-EI-MS identification data	0:27	GC-EI-MS identification data of neutral sugars of polysaccharides	0:64	GC-EI-MS identification data of neutral sugars of polysaccharides extracted from Zizyphus lotus fruit.
29896535	1	16	from	fruit	281:285	arg1	polysaccharides					245:259	the water soluble polysaccharides	227:259	the water soluble polysaccharides from Zizyphus lotus fruit	227:285	Gas chromatography coupled to mass spectrometer (GC-MS) was used to identify and to quantify neutral sugars that constitute the water soluble polysaccharides from Zizyphus lotus fruit.
29896535	4	17	theme	calibration	646:656	arg1	curves					658:663	calibration curves	646:663	calibration curves	646:663	All sugars were quantified in TIC (Total Ion Current) mode using calibration curves.
29896535	0	18	dep	Zizyphus	81:88	arg1	lotus					90:94	lotus	90:94	lotus	90:94	GC-EI-MS identification data of neutral sugars of polysaccharides extracted from Zizyphus lotus fruit.
29896535	2	19	theme	monosaccharides	368:382	arg1	units					384:388	the monosaccharides units	364:388	the monosaccharides units of extracted polysaccharides that were released by hydrolysis method	364:457	The trimethylsilyl (TMS) method was successfully used for derivatization of the monosaccharides units of extracted polysaccharides that were released by hydrolysis method.
29896535	2	20	theme	hydrolysis	441:450	arg1	method					452:457	hydrolysis method	441:457	hydrolysis method	441:457	The trimethylsilyl (TMS) method was successfully used for derivatization of the monosaccharides units of extracted polysaccharides that were released by hydrolysis method.
29896535	2	21	used	used	337:340	arg2	method					313:318	The trimethylsilyl (TMS) method	288:318	The trimethylsilyl (TMS) method	288:318	The trimethylsilyl (TMS) method was successfully used for derivatization of the monosaccharides units of extracted polysaccharides that were released by hydrolysis method.
29896535	1	22	theme	Gas	103:105	arg1	chromatography					107:120	Gas chromatography	103:120	Gas chromatography coupled to mass spectrometer (GC-MS)	103:157	Gas chromatography coupled to mass spectrometer (GC-MS) was used to identify and to quantify neutral sugars that constitute the water soluble polysaccharides from Zizyphus lotus fruit.
29896535	2	23	theme	TMS	308:310	arg1	method					313:318	The trimethylsilyl (TMS) method	288:318	The trimethylsilyl (TMS) method	288:318	The trimethylsilyl (TMS) method was successfully used for derivatization of the monosaccharides units of extracted polysaccharides that were released by hydrolysis method.
29896535	5	24	theme	Optimization	686:697	arg1	extraction					699:708	Optimization extraction	686:708	"Optimization extraction of polysaccharide from Tunisian Zizyphus lotus fruit by response surface methodology: Composition and antioxidant activity" (Mkadmini Hammi et al., 2016) [1]	685:866	Data is related to "Optimization extraction of polysaccharide from Tunisian Zizyphus lotus fruit by response surface methodology: Composition and antioxidant activity" (Mkadmini Hammi et al., 2016) [1].
29896535	2	25	theme	trimethylsilyl	292:305	arg1	method					313:318	The trimethylsilyl (TMS) method	288:318	The trimethylsilyl (TMS) method	288:318	The trimethylsilyl (TMS) method was successfully used for derivatization of the monosaccharides units of extracted polysaccharides that were released by hydrolysis method.
29896535	4	26	theme	Ion	622:624	arg1	Current					626:632	Total Ion Current	616:632	Total Ion Current	616:632	All sugars were quantified in TIC (Total Ion Current) mode using calibration curves.
29896535	0	27	theme	sugars	40:45	arg1	data					24:27	GC-EI-MS identification data	0:27	GC-EI-MS identification data of neutral sugars of polysaccharides	0:64	GC-EI-MS identification data of neutral sugars of polysaccharides extracted from Zizyphus lotus fruit.
29896535	1	28	theme	neutral	196:202	arg1	sugars					204:209	neutral sugars	196:209	neutral sugars that constitute the water soluble polysaccharides from Zizyphus lotus fruit	196:285	Gas chromatography coupled to mass spectrometer (GC-MS) was used to identify and to quantify neutral sugars that constitute the water soluble polysaccharides from Zizyphus lotus fruit.
29896535	0	29	theme	neutral	32:38	arg1	sugars					40:45	neutral sugars	32:45	neutral sugars of polysaccharides	32:64	GC-EI-MS identification data of neutral sugars of polysaccharides extracted from Zizyphus lotus fruit.
29896535	5	30	theme	activity	824:831	arg1	"					832:832	Composition and antioxidant activity"	796:832	"Optimization extraction of polysaccharide from Tunisian Zizyphus lotus fruit by response surface methodology: Composition and antioxidant activity" (Mkadmini Hammi et al., 2016) [1]	685:866	Data is related to "Optimization extraction of polysaccharide from Tunisian Zizyphus lotus fruit by response surface methodology: Composition and antioxidant activity" (Mkadmini Hammi et al., 2016) [1].
29896535	5	31	from	extraction	699:708	arg1	fruit					757:761	Tunisian Zizyphus lotus fruit	733:761	Tunisian Zizyphus lotus fruit	733:761	Data is related to "Optimization extraction of polysaccharide from Tunisian Zizyphus lotus fruit by response surface methodology: Composition and antioxidant activity" (Mkadmini Hammi et al., 2016) [1].
29896535	2	32	theme	polysaccharides	403:417	arg1	units					384:388	the monosaccharides units	364:388	the monosaccharides units of extracted polysaccharides that were released by hydrolysis method	364:457	The trimethylsilyl (TMS) method was successfully used for derivatization of the monosaccharides units of extracted polysaccharides that were released by hydrolysis method.
29896535	0	33	theme	polysaccharides	50:64	arg1	sugars					40:45	neutral sugars	32:45	neutral sugars of polysaccharides	32:64	GC-EI-MS identification data of neutral sugars of polysaccharides extracted from Zizyphus lotus fruit.
29896535	3	34	theme	retention	498:506	arg1	times					508:512	their retention times	492:512	their retention times compared with those of standards and the NIST MS Spectral Library	492:578	Sugars were identified based on their retention times compared with those of standards and the NIST MS Spectral Library.
29896535	5	35	theme	Composition	796:806	arg1	"					832:832	Composition and antioxidant activity"	796:832	"Optimization extraction of polysaccharide from Tunisian Zizyphus lotus fruit by response surface methodology: Composition and antioxidant activity" (Mkadmini Hammi et al., 2016) [1]	685:866	Data is related to "Optimization extraction of polysaccharide from Tunisian Zizyphus lotus fruit by response surface methodology: Composition and antioxidant activity" (Mkadmini Hammi et al., 2016) [1].
29896535	5	36	dep	Zizyphus	742:749	arg1	lotus					751:755	lotus	751:755	lotus	751:755	Data is related to "Optimization extraction of polysaccharide from Tunisian Zizyphus lotus fruit by response surface methodology: Composition and antioxidant activity" (Mkadmini Hammi et al., 2016) [1].
29896535	1	37	dep	Zizyphus	266:273	arg1	lotus					275:279	lotus	275:279	lotus	275:279	Gas chromatography coupled to mass spectrometer (GC-MS) was used to identify and to quantify neutral sugars that constitute the water soluble polysaccharides from Zizyphus lotus fruit.
29896535	5	38	dep	Hammi	844:848	arg1	[1					864:865	[1	864:865	Mkadmini Hammi et al., 2016) [1	835:865	Data is related to "Optimization extraction of polysaccharide from Tunisian Zizyphus lotus fruit by response surface methodology: Composition and antioxidant activity" (Mkadmini Hammi et al., 2016) [1].
29896535	1	39	theme	mass	133:136	arg1	GC-MS					152:156	GC-MS	152:156	GC-MS	152:156	Gas chromatography coupled to mass spectrometer (GC-MS) was used to identify and to quantify neutral sugars that constitute the water soluble polysaccharides from Zizyphus lotus fruit.
29896535	1	39	theme	mass	133:136	arg1	spectrometer					138:149	mass spectrometer	133:149	mass spectrometer (GC-MS)	133:157	Gas chromatography coupled to mass spectrometer (GC-MS) was used to identify and to quantify neutral sugars that constitute the water soluble polysaccharides from Zizyphus lotus fruit.
29896535	3	40	theme	MS	560:561	arg1	Library					572:578	the NIST MS Spectral Library	551:578	the NIST MS Spectral Library	551:578	Sugars were identified based on their retention times compared with those of standards and the NIST MS Spectral Library.
29896535	4	41	theme	TIC	611:613	arg1	mode					635:638	TIC (Total Ion Current) mode	611:638	TIC (Total Ion Current) mode	611:638	All sugars were quantified in TIC (Total Ion Current) mode using calibration curves.
29896535	4	42	dep	TIC	611:613	arg1	Current					626:632	Total Ion Current	616:632	Total Ion Current	616:632	All sugars were quantified in TIC (Total Ion Current) mode using calibration curves.
29896535	5	43	dep	extraction	699:708	arg1	"					832:832	Composition and antioxidant activity"	796:832	"Optimization extraction of polysaccharide from Tunisian Zizyphus lotus fruit by response surface methodology: Composition and antioxidant activity" (Mkadmini Hammi et al., 2016) [1]	685:866	Data is related to "Optimization extraction of polysaccharide from Tunisian Zizyphus lotus fruit by response surface methodology: Composition and antioxidant activity" (Mkadmini Hammi et al., 2016) [1].
29896535	5	44	from	fruit	757:761	arg1	polysaccharide					713:726	polysaccharide	713:726	polysaccharide from Tunisian Zizyphus lotus fruit	713:761	Data is related to "Optimization extraction of polysaccharide from Tunisian Zizyphus lotus fruit by response surface methodology: Composition and antioxidant activity" (Mkadmini Hammi et al., 2016) [1].
29896535	5	44	from	fruit	757:761	arg1	extraction					699:708	Optimization extraction	686:708	"Optimization extraction of polysaccharide from Tunisian Zizyphus lotus fruit by response surface methodology: Composition and antioxidant activity" (Mkadmini Hammi et al., 2016) [1]	685:866	Data is related to "Optimization extraction of polysaccharide from Tunisian Zizyphus lotus fruit by response surface methodology: Composition and antioxidant activity" (Mkadmini Hammi et al., 2016) [1].
29896535	4	45	theme	Total	616:620	arg1	Current					626:632	Total Ion Current	616:632	Total Ion Current	616:632	All sugars were quantified in TIC (Total Ion Current) mode using calibration curves.
29896535	1	46	used	used	163:166	arg2	chromatography					107:120	Gas chromatography	103:120	Gas chromatography coupled to mass spectrometer (GC-MS)	103:157	Gas chromatography coupled to mass spectrometer (GC-MS) was used to identify and to quantify neutral sugars that constitute the water soluble polysaccharides from Zizyphus lotus fruit.
30809437	10	0	dep	it	1208:1209	arg1	drug					1223:1226	a potential drug	1211:1226	it a potential drug for anticancer treatment in the future	1208:1265	This might make it a potential drug for anticancer treatment in the future.
30809437	6	1	theme	main	817:820	arg1	fucose					778:783	fucose	778:783	fucose	778:783	The results showed that PTP was a sulfated polysaccharide with a high Mw of 4,810 kDa, and xylose, fucose, glucose and galactose were the main monosaccharides.
30809437	6	1	theme	main	817:820	arg1	galactose					798:806	galactose	798:806	galactose	798:806	The results showed that PTP was a sulfated polysaccharide with a high Mw of 4,810 kDa, and xylose, fucose, glucose and galactose were the main monosaccharides.
30809437	6	1	theme	main	817:820	arg1	glucose					786:792	glucose	786:792	glucose	786:792	The results showed that PTP was a sulfated polysaccharide with a high Mw of 4,810 kDa, and xylose, fucose, glucose and galactose were the main monosaccharides.
30809437	6	1	theme	main	817:820	arg1	monosaccharides					822:836	the main monosaccharides	813:836	the main monosaccharides	813:836	The results showed that PTP was a sulfated polysaccharide with a high Mw of 4,810 kDa, and xylose, fucose, glucose and galactose were the main monosaccharides.
30809437	6	1	theme	main	817:820	arg1	xylose					770:775	xylose	770:775	xylose	770:775	The results showed that PTP was a sulfated polysaccharide with a high Mw of 4,810 kDa, and xylose, fucose, glucose and galactose were the main monosaccharides.
30809437	1	2	theme	unicellular	166:176	arg1	Microalgae					143:152	Microalgae	143:152	Microalgae	143:152	Microalgae, eukaryotic unicellular plants, are increasing in demand due to their use as nutraceutical and food supplements.
30809437	1	2	theme	unicellular	166:176	arg1	plants					178:183	eukaryotic unicellular plants	155:183	eukaryotic unicellular plants	155:183	Microalgae, eukaryotic unicellular plants, are increasing in demand due to their use as nutraceutical and food supplements.
30809437	0	3	theme	HepG2	111:115	arg1	polysaccharides					9:23	Sulfated polysaccharides	0:23	Sulfated polysaccharides from Phaeodactylum tricornutum: isolation, structural characteristics, and inhibiting HepG2 growth activity in vitro.	0:141	Sulfated polysaccharides from Phaeodactylum tricornutum: isolation, structural characteristics, and inhibiting HepG2 growth activity in vitro.
30809437	0	3	theme	HepG2	111:115	arg1	activity					124:131	inhibiting HepG2 growth activity	100:131	inhibiting HepG2 growth activity	100:131	Sulfated polysaccharides from Phaeodactylum tricornutum: isolation, structural characteristics, and inhibiting HepG2 growth activity in vitro.
30809437	7	4	dep	manner	899:904	arg1	%					918:918	up to 60.37%	907:918	up to 60.37%	907:918	PTP has significant anticancer activity in a dose-dependent manner (up to 60.37% at 250 ug/mL) according to MTT assays.
30809437	0	5	theme	inhibiting	100:109	arg1	polysaccharides					9:23	Sulfated polysaccharides	0:23	Sulfated polysaccharides from Phaeodactylum tricornutum: isolation, structural characteristics, and inhibiting HepG2 growth activity in vitro.	0:141	Sulfated polysaccharides from Phaeodactylum tricornutum: isolation, structural characteristics, and inhibiting HepG2 growth activity in vitro.
30809437	0	5	theme	inhibiting	100:109	arg1	activity					124:131	inhibiting HepG2 growth activity	100:131	inhibiting HepG2 growth activity	100:131	Sulfated polysaccharides from Phaeodactylum tricornutum: isolation, structural characteristics, and inhibiting HepG2 growth activity in vitro.
30809437	5	6	theme	anticancer	643:652	arg1	activity					654:661	its anticancer activity	639:661	its anticancer activity	639:661	Therefore, in this work, we extracted polysaccharides from Phaeodactylum tricornutum (PTP), characterized the chemical composition and structure, and investigated its anticancer activity on HepG2 cells.
30809437	5	7	theme	chemical	586:593	arg1	composition					595:605	the chemical composition	582:605	the chemical composition	582:605	Therefore, in this work, we extracted polysaccharides from Phaeodactylum tricornutum (PTP), characterized the chemical composition and structure, and investigated its anticancer activity on HepG2 cells.
30809437	2	8	theme	different	282:290	arg1	kinds					292:296	different kinds	282:296	different kinds of biologically active components such as polysaccharides	282:354	They consisted different kinds of biologically active components such as polysaccharides.
30809437	0	9	theme	growth	117:122	arg1	polysaccharides					9:23	Sulfated polysaccharides	0:23	Sulfated polysaccharides from Phaeodactylum tricornutum: isolation, structural characteristics, and inhibiting HepG2 growth activity in vitro.	0:141	Sulfated polysaccharides from Phaeodactylum tricornutum: isolation, structural characteristics, and inhibiting HepG2 growth activity in vitro.
30809437	0	9	theme	growth	117:122	arg1	activity					124:131	inhibiting HepG2 growth activity	100:131	inhibiting HepG2 growth activity	100:131	Sulfated polysaccharides from Phaeodactylum tricornutum: isolation, structural characteristics, and inhibiting HepG2 growth activity in vitro.
30809437	7	10	contain	has	843:845	arg2	activity					870:877	significant anticancer activity	847:877	significant anticancer activity	847:877	PTP has significant anticancer activity in a dose-dependent manner (up to 60.37% at 250 ug/mL) according to MTT assays.
30809437	7	10	contain	has	843:845	arg1	PTP					839:841	PTP	839:841	PTP	839:841	PTP has significant anticancer activity in a dose-dependent manner (up to 60.37% at 250 ug/mL) according to MTT assays.
30809437	7	11	theme	dose-dependent	884:897	arg1	manner					899:904	a dose-dependent manner	882:904	a dose-dependent manner (up to 60.37% at 250 ug/mL) according to MTT assays	882:956	PTP has significant anticancer activity in a dose-dependent manner (up to 60.37% at 250 ug/mL) according to MTT assays.
30809437	0	12	theme	Sulfated	0:7	arg1	isolation					57:65	isolation	57:65	isolation	57:65	Sulfated polysaccharides from Phaeodactylum tricornutum: isolation, structural characteristics, and inhibiting HepG2 growth activity in vitro.
30809437	0	12	theme	Sulfated	0:7	arg1	polysaccharides					9:23	Sulfated polysaccharides	0:23	Sulfated polysaccharides from Phaeodactylum tricornutum: isolation, structural characteristics, and inhibiting HepG2 growth activity in vitro.	0:141	Sulfated polysaccharides from Phaeodactylum tricornutum: isolation, structural characteristics, and inhibiting HepG2 growth activity in vitro.
30809437	0	12	theme	Sulfated	0:7	arg1	activity					124:131	inhibiting HepG2 growth activity	100:131	inhibiting HepG2 growth activity	100:131	Sulfated polysaccharides from Phaeodactylum tricornutum: isolation, structural characteristics, and inhibiting HepG2 growth activity in vitro.
30809437	0	13	from	tricornutum	44:54	arg1	isolation					57:65	isolation	57:65	isolation	57:65	Sulfated polysaccharides from Phaeodactylum tricornutum: isolation, structural characteristics, and inhibiting HepG2 growth activity in vitro.
30809437	0	13	from	tricornutum	44:54	arg1	polysaccharides					9:23	Sulfated polysaccharides	0:23	Sulfated polysaccharides from Phaeodactylum tricornutum: isolation, structural characteristics, and inhibiting HepG2 growth activity in vitro.	0:141	Sulfated polysaccharides from Phaeodactylum tricornutum: isolation, structural characteristics, and inhibiting HepG2 growth activity in vitro.
30809437	0	13	from	tricornutum	44:54	arg1	activity					124:131	inhibiting HepG2 growth activity	100:131	inhibiting HepG2 growth activity	100:131	Sulfated polysaccharides from Phaeodactylum tricornutum: isolation, structural characteristics, and inhibiting HepG2 growth activity in vitro.
30809437	3	14	theme	leading	390:396	arg1	cancer					376:381	cancer	376:381	cancer	376:381	On the other hand, cancer is the leading cause of death globally.
30809437	3	14	theme	leading	390:396	arg1	cause					398:402	the leading cause	386:402	the leading cause of death	386:411	On the other hand, cancer is the leading cause of death globally.
30809437	2	15	theme	components	321:330	arg1	kinds					292:296	different kinds	282:296	different kinds of biologically active components such as polysaccharides	282:354	They consisted different kinds of biologically active components such as polysaccharides.
30809437	10	16	theme	anticancer	1232:1241	arg1	treatment					1243:1251	anticancer treatment	1232:1251	anticancer treatment	1232:1251	This might make it a potential drug for anticancer treatment in the future.
30809437	7	17	from	ug/mL	927:931	arg1	%					918:918	up to 60.37%	907:918	up to 60.37%	907:918	PTP has significant anticancer activity in a dose-dependent manner (up to 60.37% at 250 ug/mL) according to MTT assays.
30809437	6	18	theme	kDa	761:763	arg1	Mw					749:750	a high Mw	742:750	a high Mw of 4,810 kDa	742:763	The results showed that PTP was a sulfated polysaccharide with a high Mw of 4,810 kDa, and xylose, fucose, glucose and galactose were the main monosaccharides.
30809437	2	19	theme	active	314:319	arg1	components					321:330	biologically active components	301:330	biologically active components such as polysaccharides	301:354	They consisted different kinds of biologically active components such as polysaccharides.
30809437	2	19	theme	active	314:319	arg1	polysaccharides					340:354	polysaccharides	340:354	polysaccharides	340:354	They consisted different kinds of biologically active components such as polysaccharides.
30809437	1	20	dep	nutraceutical	231:243	arg1	supplements					254:264	supplements	254:264	supplements	254:264	Microalgae, eukaryotic unicellular plants, are increasing in demand due to their use as nutraceutical and food supplements.
30809437	10	21	theme	potential	1213:1221	arg1	drug					1223:1226	a potential drug	1211:1226	it a potential drug for anticancer treatment in the future	1208:1265	This might make it a potential drug for anticancer treatment in the future.
30809437	3	22	theme	death	407:411	arg1	cancer					376:381	cancer	376:381	cancer	376:381	On the other hand, cancer is the leading cause of death globally.
30809437	3	22	theme	death	407:411	arg1	cause					398:402	the leading cause	386:402	the leading cause of death	386:411	On the other hand, cancer is the leading cause of death globally.
30809437	7	23	dep	60.37	913:917	arg1	to					910:911	to	910:911	to	910:911	PTP has significant anticancer activity in a dose-dependent manner (up to 60.37% at 250 ug/mL) according to MTT assays.
30809437	9	24	theme	cells	1185:1189	arg1	mitosis					1168:1174	mitosis	1168:1174	mitosis	1168:1174	The results showed that it exhibited anticancer effect mainly through the induction of apoptosis without affecting the cycle and mitosis of HepG2 cells.
30809437	9	24	theme	cells	1185:1189	arg1	cycle					1158:1162	cycle	1158:1162	cycle	1158:1162	The results showed that it exhibited anticancer effect mainly through the induction of apoptosis without affecting the cycle and mitosis of HepG2 cells.
30809437	4	25	theme	efficient	447:455	arg1	method					457:462	no efficient method	444:462	no efficient method to cure it	444:473	At present, there is no efficient method to cure it.
30809437	3	26	theme	other	364:368	arg1	hand					370:373	the other hand	360:373	the other hand	360:373	On the other hand, cancer is the leading cause of death globally.
30809437	7	27	theme	MTT	947:949	arg1	assays					951:956	MTT assays	947:956	MTT assays	947:956	PTP has significant anticancer activity in a dose-dependent manner (up to 60.37% at 250 ug/mL) according to MTT assays.
30809437	8	28	theme	anticancer	1018:1027	arg1	activity					1029:1036	its anticancer activity	1014:1036	its anticancer activity	1014:1036	Furthermore, cycle analysis was carried out to explain its anticancer activity.
30809437	9	29	theme	HepG2	1179:1183	arg1	cells					1185:1189	HepG2 cells	1179:1189	HepG2 cells	1179:1189	The results showed that it exhibited anticancer effect mainly through the induction of apoptosis without affecting the cycle and mitosis of HepG2 cells.
30809437	8	30	theme	cycle	972:976	arg1	analysis					978:985	cycle analysis	972:985	cycle analysis	972:985	Furthermore, cycle analysis was carried out to explain its anticancer activity.
30809437	7	31	theme	anticancer	859:868	arg1	activity					870:877	significant anticancer activity	847:877	significant anticancer activity	847:877	PTP has significant anticancer activity in a dose-dependent manner (up to 60.37% at 250 ug/mL) according to MTT assays.
30809437	6	32	theme	high	744:747	arg1	Mw					749:750	a high Mw	742:750	a high Mw of 4,810 kDa	742:763	The results showed that PTP was a sulfated polysaccharide with a high Mw of 4,810 kDa, and xylose, fucose, glucose and galactose were the main monosaccharides.
30809437	5	33	theme	HepG2	666:670	arg1	cells					672:676	HepG2 cells	666:676	HepG2 cells	666:676	Therefore, in this work, we extracted polysaccharides from Phaeodactylum tricornutum (PTP), characterized the chemical composition and structure, and investigated its anticancer activity on HepG2 cells.
30809437	9	34	theme	apoptosis	1126:1134	arg1	induction					1113:1121	the induction	1109:1121	the induction of apoptosis	1109:1134	The results showed that it exhibited anticancer effect mainly through the induction of apoptosis without affecting the cycle and mitosis of HepG2 cells.
30809437	10	35	from	drug	1223:1226	arg1	future					1260:1265	future	1260:1265	future	1260:1265	This might make it a potential drug for anticancer treatment in the future.
30809437	6	36	theme	sulfated	713:720	arg1	polysaccharide					722:735	a sulfated polysaccharide	711:735	a sulfated polysaccharide with a high Mw of 4,810 kDa	711:763	The results showed that PTP was a sulfated polysaccharide with a high Mw of 4,810 kDa, and xylose, fucose, glucose and galactose were the main monosaccharides.
30809437	6	36	theme	sulfated	713:720	arg1	PTP					703:705	PTP	703:705	PTP	703:705	The results showed that PTP was a sulfated polysaccharide with a high Mw of 4,810 kDa, and xylose, fucose, glucose and galactose were the main monosaccharides.
30809437	5	37	from	tricornutum	549:559	arg1	polysaccharides					514:528	polysaccharides	514:528	polysaccharides from Phaeodactylum tricornutum (PTP)	514:565	Therefore, in this work, we extracted polysaccharides from Phaeodactylum tricornutum (PTP), characterized the chemical composition and structure, and investigated its anticancer activity on HepG2 cells.
30809437	6	38	with	polysaccharide	722:735	arg1	Mw					749:750	a high Mw	742:750	a high Mw of 4,810 kDa	742:763	The results showed that PTP was a sulfated polysaccharide with a high Mw of 4,810 kDa, and xylose, fucose, glucose and galactose were the main monosaccharides.
30809437	0	39	dep	polysaccharides	9:23	arg1	polysaccharides					9:23	Sulfated polysaccharides	0:23	Sulfated polysaccharides from Phaeodactylum tricornutum: isolation, structural characteristics, and inhibiting HepG2 growth activity in vitro.	0:141	Sulfated polysaccharides from Phaeodactylum tricornutum: isolation, structural characteristics, and inhibiting HepG2 growth activity in vitro.
30809437	0	39	dep	polysaccharides	9:23	arg1	isolation					57:65	isolation	57:65	isolation	57:65	Sulfated polysaccharides from Phaeodactylum tricornutum: isolation, structural characteristics, and inhibiting HepG2 growth activity in vitro.
30809437	0	39	dep	polysaccharides	9:23	arg1	characteristics					79:93	structural characteristics	68:93	structural characteristics	68:93	Sulfated polysaccharides from Phaeodactylum tricornutum: isolation, structural characteristics, and inhibiting HepG2 growth activity in vitro.
30809437	0	39	dep	polysaccharides	9:23	arg1	activity					124:131	inhibiting HepG2 growth activity	100:131	inhibiting HepG2 growth activity	100:131	Sulfated polysaccharides from Phaeodactylum tricornutum: isolation, structural characteristics, and inhibiting HepG2 growth activity in vitro.
30809437	9	40	theme	anticancer	1076:1085	arg1	effect					1087:1092	anticancer effect	1076:1092	anticancer effect	1076:1092	The results showed that it exhibited anticancer effect mainly through the induction of apoptosis without affecting the cycle and mitosis of HepG2 cells.
30809437	6	41	with	monosaccharides	822:836	arg1	Mw					749:750	a high Mw	742:750	a high Mw of 4,810 kDa	742:763	The results showed that PTP was a sulfated polysaccharide with a high Mw of 4,810 kDa, and xylose, fucose, glucose and galactose were the main monosaccharides.
30809437	7	42	theme	significant	847:857	arg1	activity					870:877	significant anticancer activity	847:877	significant anticancer activity	847:877	PTP has significant anticancer activity in a dose-dependent manner (up to 60.37% at 250 ug/mL) according to MTT assays.
30809437	9	43	dep	cycle	1158:1162	arg1	the					1154:1156	the	1154:1156	the	1154:1156	The results showed that it exhibited anticancer effect mainly through the induction of apoptosis without affecting the cycle and mitosis of HepG2 cells.
30809437	0	44	theme	structural	68:77	arg1	characteristics					79:93	structural characteristics	68:93	structural characteristics	68:93	Sulfated polysaccharides from Phaeodactylum tricornutum: isolation, structural characteristics, and inhibiting HepG2 growth activity in vitro.
30809437	3	45	from	cause	398:402	arg1	hand					370:373	the other hand	360:373	the other hand	360:373	On the other hand, cancer is the leading cause of death globally.
30809437	1	46	theme	eukaryotic	155:164	arg1	Microalgae					143:152	Microalgae	143:152	Microalgae	143:152	Microalgae, eukaryotic unicellular plants, are increasing in demand due to their use as nutraceutical and food supplements.
30809437	1	46	theme	eukaryotic	155:164	arg1	plants					178:183	eukaryotic unicellular plants	155:183	eukaryotic unicellular plants	155:183	Microalgae, eukaryotic unicellular plants, are increasing in demand due to their use as nutraceutical and food supplements.
29966293	14	0	theme	physiological	2257:2269	arg1	adaption					2271:2278	exercise physiological adaption	2248:2278	exercise physiological adaption	2248:2278	Furthermore, the TTDF could be applied to not only the health promotion but also improvement of exercise physiological adaption and the TTFD could be further considered as potential ergogenic aids combined with different nutrient strategy.
29966293	1	1	theme	pyruvate	251:258	arg1	dehydrogenase					260:272	pyruvate dehydrogenase	251:272	pyruvate dehydrogenase	251:272	Thiamine, named as vitamin B1, is an important cofactor for the critical enzymes regarding to glucose metabolism, like transketolase, pyruvate dehydrogenase, and alpha-ketoglutarate dehydrogenase.
29966293	11	2	theme	BUN	1713:1715	arg1	indexes					1724:1730	the BUN and CK indexes	1709:1730	the BUN and CK indexes after extended exercise	1709:1754	The TTFD supplementation significantly mitigated the BUN and CK indexes after extended exercise and elevated the glycogen content in the liver and muscle tissues.
29966293	12	3	dep	administration	1900:1913	arg1	toxicity					1950:1957	subacute toxicity	1941:1957	daily administration of TTFD for over 6 weeks (subacute toxicity)	1894:1958	According to body composition, biochemical and histopathological data, daily administration of TTFD for over 6 weeks (subacute toxicity) also demonstrated reasonable safety results for long-term and adequate supplementation.
29966293	7	4	theme	biochemical	1128:1138	arg1	nitrogen					1188:1195	blood urine nitrogen	1176:1195	blood urine nitrogen (BUN)	1176:1201	Various fatigue-associated biochemical variables such as lactate, glucose, blood urine nitrogen (BUN) or creatine kinase (CK), were also assessed.
29966293	7	4	theme	biochemical	1128:1138	arg1	kinase					1215:1220	creatine kinase	1206:1220	creatine kinase (CK)	1206:1225	Various fatigue-associated biochemical variables such as lactate, glucose, blood urine nitrogen (BUN) or creatine kinase (CK), were also assessed.
29966293	7	4	theme	biochemical	1128:1138	arg1	glucose					1167:1173	glucose	1167:1173	glucose	1167:1173	Various fatigue-associated biochemical variables such as lactate, glucose, blood urine nitrogen (BUN) or creatine kinase (CK), were also assessed.
29966293	7	4	theme	biochemical	1128:1138	arg1	variables					1140:1148	Various fatigue-associated biochemical variables	1101:1148	Various fatigue-associated biochemical variables such as lactate, glucose, blood urine nitrogen (BUN) or creatine kinase (CK),	1101:1226	Various fatigue-associated biochemical variables such as lactate, glucose, blood urine nitrogen (BUN) or creatine kinase (CK), were also assessed.
29966293	7	4	theme	biochemical	1128:1138	arg1	lactate					1158:1164	lactate	1158:1164	lactate	1158:1164	Various fatigue-associated biochemical variables such as lactate, glucose, blood urine nitrogen (BUN) or creatine kinase (CK), were also assessed.
29966293	3	5	theme	higher	566:571	arg1	supplements					582:592	higher thiamine supplements	566:592	higher thiamine supplements for efficient energy metabolism	566:624	Higher physical activities are associated with higher thiamine supplements for efficient energy metabolism.
29966293	12	6	theme	long-term	2008:2016	arg1	supplementation					2031:2045	long-term and adequate supplementation	2008:2045	long-term and adequate supplementation	2008:2045	According to body composition, biochemical and histopathological data, daily administration of TTFD for over 6 weeks (subacute toxicity) also demonstrated reasonable safety results for long-term and adequate supplementation.
29966293	7	7	theme	Various	1101:1107	arg1	nitrogen					1188:1195	blood urine nitrogen	1176:1195	blood urine nitrogen (BUN)	1176:1201	Various fatigue-associated biochemical variables such as lactate, glucose, blood urine nitrogen (BUN) or creatine kinase (CK), were also assessed.
29966293	7	7	theme	Various	1101:1107	arg1	kinase					1215:1220	creatine kinase	1206:1220	creatine kinase (CK)	1206:1225	Various fatigue-associated biochemical variables such as lactate, glucose, blood urine nitrogen (BUN) or creatine kinase (CK), were also assessed.
29966293	7	7	theme	Various	1101:1107	arg1	glucose					1167:1173	glucose	1167:1173	glucose	1167:1173	Various fatigue-associated biochemical variables such as lactate, glucose, blood urine nitrogen (BUN) or creatine kinase (CK), were also assessed.
29966293	7	7	theme	Various	1101:1107	arg1	variables					1140:1148	Various fatigue-associated biochemical variables	1101:1148	Various fatigue-associated biochemical variables such as lactate, glucose, blood urine nitrogen (BUN) or creatine kinase (CK),	1101:1226	Various fatigue-associated biochemical variables such as lactate, glucose, blood urine nitrogen (BUN) or creatine kinase (CK), were also assessed.
29966293	7	7	theme	Various	1101:1107	arg1	lactate					1158:1164	lactate	1158:1164	lactate	1158:1164	Various fatigue-associated biochemical variables such as lactate, glucose, blood urine nitrogen (BUN) or creatine kinase (CK), were also assessed.
29966293	2	8	theme	tetrahydrofurfuryl	327:344	arg1	derivative					368:377	a derivative	366:377	a derivative of thiamine	366:389	The thiamine tetrahydrofurfuryl disulfide (TTFD) is a derivative of thiamine with higher bioavailability and solubility than thiamine and has been widely applied to health maintenance and disease therapy.
29966293	2	8	theme	tetrahydrofurfuryl	327:344	arg1	TTFD					357:360	TTFD	357:360	TTFD	357:360	The thiamine tetrahydrofurfuryl disulfide (TTFD) is a derivative of thiamine with higher bioavailability and solubility than thiamine and has been widely applied to health maintenance and disease therapy.
29966293	2	8	theme	tetrahydrofurfuryl	327:344	arg1	disulfide					346:354	The thiamine tetrahydrofurfuryl disulfide	314:354	The thiamine tetrahydrofurfuryl disulfide (TTFD)	314:361	The thiamine tetrahydrofurfuryl disulfide (TTFD) is a derivative of thiamine with higher bioavailability and solubility than thiamine and has been widely applied to health maintenance and disease therapy.
29966293	1	9	theme	critical	181:188	arg1	enzymes					190:196	the critical enzymes	177:196	the critical enzymes	177:196	Thiamine, named as vitamin B1, is an important cofactor for the critical enzymes regarding to glucose metabolism, like transketolase, pyruvate dehydrogenase, and alpha-ketoglutarate dehydrogenase.
29966293	12	10	theme	adequate	2022:2029	arg1	supplementation					2031:2045	long-term and adequate supplementation	2008:2045	long-term and adequate supplementation	2008:2045	According to body composition, biochemical and histopathological data, daily administration of TTFD for over 6 weeks (subacute toxicity) also demonstrated reasonable safety results for long-term and adequate supplementation.
29966293	4	11	theme	beneficial	668:677	arg1	dose					654:657	the effective dose	640:657	the effective dose	640:657	Furthermore, the effective dose of TTFD, beneficial to exercise physiological adaption and performance, still be further validated and the safety evaluation were also an important issue to be considered for potential application.
29966293	10	12	theme	clearance	1610:1618	arg1	rate					1620:1623	clearance rate	1610:1623	clearance rate	1610:1623	TTFD supplementation significantly increased the endurance and grip strength and demonstrated beneficial effects on lactate production and clearance rate after an acute exercise challenge.
29966293	3	13	theme	efficient	598:606	arg1	metabolism					615:624	efficient energy metabolism	598:624	efficient energy metabolism	598:624	Higher physical activities are associated with higher thiamine supplements for efficient energy metabolism.
29966293	5	14	theme	Cancer	875:880	arg1	Research					882:889	Cancer Research	875:889	Cancer Research	875:889	ICR (Institute of Cancer Research) strain mice were allocated as 0, 50, 100, and 500 mg/kg dose groups and administrated by oral gavage consecutively for 6 weeks.
29966293	12	15	theme	reasonable	1978:1987	arg1	results					1996:2002	reasonable safety results	1978:2002	reasonable safety results for long-term and adequate supplementation	1978:2045	According to body composition, biochemical and histopathological data, daily administration of TTFD for over 6 weeks (subacute toxicity) also demonstrated reasonable safety results for long-term and adequate supplementation.
29966293	4	16	theme	exercise	682:689	arg1	adaption					705:712	exercise physiological adaption	682:712	exercise physiological adaption	682:712	Furthermore, the effective dose of TTFD, beneficial to exercise physiological adaption and performance, still be further validated and the safety evaluation were also an important issue to be considered for potential application.
29966293	9	17	theme	long-term	1391:1399	arg1	supplementation					1401:1415	long-term supplementation	1391:1415	long-term supplementation	1391:1415	Toxicity assessments for long-term supplementation were also further evaluated for safety consideration.
29966293	7	18	theme	urine	1182:1186	arg1	nitrogen					1188:1195	blood urine nitrogen	1176:1195	blood urine nitrogen (BUN)	1176:1201	Various fatigue-associated biochemical variables such as lactate, glucose, blood urine nitrogen (BUN) or creatine kinase (CK), were also assessed.
29966293	7	18	theme	urine	1182:1186	arg1	BUN					1198:1200	BUN	1198:1200	BUN	1198:1200	Various fatigue-associated biochemical variables such as lactate, glucose, blood urine nitrogen (BUN) or creatine kinase (CK), were also assessed.
29966293	1	19	theme	glucose	211:217	arg1	metabolism					219:228	glucose metabolism	211:228	glucose metabolism	211:228	Thiamine, named as vitamin B1, is an important cofactor for the critical enzymes regarding to glucose metabolism, like transketolase, pyruvate dehydrogenase, and alpha-ketoglutarate dehydrogenase.
29966293	8	20	theme	muscle	1272:1277	arg1	glycogen					1279:1286	muscle glycogen	1272:1286	muscle glycogen	1272:1286	The levels of liver and muscle glycogen were measured as an indicator of energy storage at the end of the experiment.
29966293	10	21	theme	acute	1634:1638	arg1	challenge					1649:1657	an acute exercise challenge	1631:1657	an acute exercise challenge	1631:1657	TTFD supplementation significantly increased the endurance and grip strength and demonstrated beneficial effects on lactate production and clearance rate after an acute exercise challenge.
29966293	4	22	theme	safety	766:771	arg1	issue					807:811	an important issue	794:811	an important issue	794:811	Furthermore, the effective dose of TTFD, beneficial to exercise physiological adaption and performance, still be further validated and the safety evaluation were also an important issue to be considered for potential application.
29966293	4	22	theme	safety	766:771	arg1	evaluation					773:782	the safety evaluation	762:782	the safety evaluation	762:782	Furthermore, the effective dose of TTFD, beneficial to exercise physiological adaption and performance, still be further validated and the safety evaluation were also an important issue to be considered for potential application.
29966293	6	23	theme	Physical	1020:1027	arg1	strength					1055:1062	grip strength	1050:1062	grip strength	1050:1062	Physical activities including grip strength and aerobic endurance were measured.
29966293	6	23	theme	Physical	1020:1027	arg1	endurance					1076:1084	aerobic endurance	1068:1084	aerobic endurance	1068:1084	Physical activities including grip strength and aerobic endurance were measured.
29966293	6	23	theme	Physical	1020:1027	arg1	activities					1029:1038	Physical activities	1020:1038	Physical activities including grip strength and aerobic endurance	1020:1084	Physical activities including grip strength and aerobic endurance were measured.
29966293	14	24	theme	nutrient	2373:2380	arg1	strategy					2382:2389	different nutrient strategy	2363:2389	different nutrient strategy	2363:2389	Furthermore, the TTDF could be applied to not only the health promotion but also improvement of exercise physiological adaption and the TTFD could be further considered as potential ergogenic aids combined with different nutrient strategy.
29966293	11	25	theme	glycogen	1773:1780	arg1	content					1782:1788	the glycogen content	1769:1788	the glycogen content in the liver and muscle tissues	1769:1820	The TTFD supplementation significantly mitigated the BUN and CK indexes after extended exercise and elevated the glycogen content in the liver and muscle tissues.
29966293	13	26	theme	long-term	2104:2112	arg1	supplementation					2114:2128	long-term supplementation	2104:2128	long-term supplementation with indicated doses	2104:2149	The toxicity of TTFD were also considered as safety for long-term supplementation with indicated doses.
29966293	11	27	theme	muscle	1807:1812	arg1	tissues					1814:1820	the liver and muscle tissues	1793:1820	the liver and muscle tissues	1793:1820	The TTFD supplementation significantly mitigated the BUN and CK indexes after extended exercise and elevated the glycogen content in the liver and muscle tissues.
29966293	0	28	theme	Exercise	83:90	arg1	Improvement					104:114	Exercise Performance Improvement	83:114	Exercise Performance Improvement	83:114	The Effects of Thiamine Tetrahydrofurfuryl Disulfide on Physiological Adaption and Exercise Performance Improvement.
29966293	12	29	theme	subacute	1941:1948	arg1	toxicity					1950:1957	subacute toxicity	1941:1957	daily administration of TTFD for over 6 weeks (subacute toxicity)	1894:1958	According to body composition, biochemical and histopathological data, daily administration of TTFD for over 6 weeks (subacute toxicity) also demonstrated reasonable safety results for long-term and adequate supplementation.
29966293	1	30	theme	important	154:162	arg1	cofactor					164:171	an important cofactor	151:171	an important cofactor for the critical enzymes regarding to glucose metabolism, like transketolase, pyruvate dehydrogenase, and alpha-ketoglutarate dehydrogenase	151:311	Thiamine, named as vitamin B1, is an important cofactor for the critical enzymes regarding to glucose metabolism, like transketolase, pyruvate dehydrogenase, and alpha-ketoglutarate dehydrogenase.
29966293	1	30	theme	important	154:162	arg1	Thiamine					117:124	Thiamine	117:124	Thiamine	117:124	Thiamine, named as vitamin B1, is an important cofactor for the critical enzymes regarding to glucose metabolism, like transketolase, pyruvate dehydrogenase, and alpha-ketoglutarate dehydrogenase.
29966293	8	31	theme	energy	1321:1326	arg1	storage					1328:1334	energy storage	1321:1334	energy storage	1321:1334	The levels of liver and muscle glycogen were measured as an indicator of energy storage at the end of the experiment.
29966293	11	32	theme	liver	1797:1801	arg1	tissues					1814:1820	the liver and muscle tissues	1793:1820	the liver and muscle tissues	1793:1820	The TTFD supplementation significantly mitigated the BUN and CK indexes after extended exercise and elevated the glycogen content in the liver and muscle tissues.
29966293	10	33	theme	TTFD	1471:1474	arg1	supplementation					1476:1490	TTFD supplementation	1471:1490	TTFD supplementation	1471:1490	TTFD supplementation significantly increased the endurance and grip strength and demonstrated beneficial effects on lactate production and clearance rate after an acute exercise challenge.
29966293	14	34	theme	potential	2324:2332	arg1	aids					2344:2347	potential ergogenic aids	2324:2347	potential ergogenic aids combined with different nutrient strategy	2324:2389	Furthermore, the TTDF could be applied to not only the health promotion but also improvement of exercise physiological adaption and the TTFD could be further considered as potential ergogenic aids combined with different nutrient strategy.
29966293	14	34	theme	potential	2324:2332	arg1	TTFD					2288:2291	the TTFD	2284:2291	the TTFD	2284:2291	Furthermore, the TTDF could be applied to not only the health promotion but also improvement of exercise physiological adaption and the TTFD could be further considered as potential ergogenic aids combined with different nutrient strategy.
29966293	5	35	theme	oral	981:984	arg1	gavage					986:991	oral gavage	981:991	oral gavage	981:991	ICR (Institute of Cancer Research) strain mice were allocated as 0, 50, 100, and 500 mg/kg dose groups and administrated by oral gavage consecutively for 6 weeks.
29966293	5	36	theme	ICR	857:859	arg1	mice					899:902	ICR (Institute of Cancer Research) strain mice	857:902	ICR (Institute of Cancer Research) strain mice	857:902	ICR (Institute of Cancer Research) strain mice were allocated as 0, 50, 100, and 500 mg/kg dose groups and administrated by oral gavage consecutively for 6 weeks.
29966293	5	36	theme	ICR	857:859	arg1	groups					953:958	0, 50, 100, and 500 mg/kg dose groups	922:958	0, 50, 100, and 500 mg/kg dose groups	922:958	ICR (Institute of Cancer Research) strain mice were allocated as 0, 50, 100, and 500 mg/kg dose groups and administrated by oral gavage consecutively for 6 weeks.
29966293	5	37	theme	mg/kg	942:946	arg1	mice					899:902	ICR (Institute of Cancer Research) strain mice	857:902	ICR (Institute of Cancer Research) strain mice	857:902	ICR (Institute of Cancer Research) strain mice were allocated as 0, 50, 100, and 500 mg/kg dose groups and administrated by oral gavage consecutively for 6 weeks.
29966293	5	37	theme	mg/kg	942:946	arg1	groups					953:958	0, 50, 100, and 500 mg/kg dose groups	922:958	0, 50, 100, and 500 mg/kg dose groups	922:958	ICR (Institute of Cancer Research) strain mice were allocated as 0, 50, 100, and 500 mg/kg dose groups and administrated by oral gavage consecutively for 6 weeks.
29966293	6	38	theme	aerobic	1068:1074	arg1	endurance					1076:1084	aerobic endurance	1068:1084	aerobic endurance	1068:1084	Physical activities including grip strength and aerobic endurance were measured.
29966293	11	39	theme	extended	1738:1745	arg1	exercise					1747:1754	extended exercise	1738:1754	extended exercise	1738:1754	The TTFD supplementation significantly mitigated the BUN and CK indexes after extended exercise and elevated the glycogen content in the liver and muscle tissues.
29966293	0	40	theme	Tetrahydrofurfuryl	24:41	arg1	Disulfide					43:51	Thiamine Tetrahydrofurfuryl Disulfide	15:51	Thiamine Tetrahydrofurfuryl Disulfide	15:51	The Effects of Thiamine Tetrahydrofurfuryl Disulfide on Physiological Adaption and Exercise Performance Improvement.
29966293	12	41	theme	TTFD	1918:1921	arg1	data					1888:1891	biochemical and histopathological data	1854:1891	biochemical and histopathological data	1854:1891	According to body composition, biochemical and histopathological data, daily administration of TTFD for over 6 weeks (subacute toxicity) also demonstrated reasonable safety results for long-term and adequate supplementation.
29966293	12	41	theme	TTFD	1918:1921	arg1	administration					1900:1913	daily administration	1894:1913	daily administration of TTFD for over 6 weeks (subacute toxicity)	1894:1958	According to body composition, biochemical and histopathological data, daily administration of TTFD for over 6 weeks (subacute toxicity) also demonstrated reasonable safety results for long-term and adequate supplementation.
29966293	10	42	from	effects	1576:1582	arg1	production					1595:1604	lactate production	1587:1604	lactate production	1587:1604	TTFD supplementation significantly increased the endurance and grip strength and demonstrated beneficial effects on lactate production and clearance rate after an acute exercise challenge.
29966293	10	42	from	effects	1576:1582	arg1	rate					1620:1623	clearance rate	1610:1623	clearance rate	1610:1623	TTFD supplementation significantly increased the endurance and grip strength and demonstrated beneficial effects on lactate production and clearance rate after an acute exercise challenge.
29966293	2	43	theme	health	479:484	arg1	maintenance					486:496	health maintenance	479:496	health maintenance	479:496	The thiamine tetrahydrofurfuryl disulfide (TTFD) is a derivative of thiamine with higher bioavailability and solubility than thiamine and has been widely applied to health maintenance and disease therapy.
29966293	13	44	theme	TTFD	2064:2067	arg1	safety					2093:2098	safety	2093:2098	safety	2093:2098	The toxicity of TTFD were also considered as safety for long-term supplementation with indicated doses.
29966293	13	44	theme	TTFD	2064:2067	arg1	toxicity					2052:2059	The toxicity	2048:2059	The toxicity of TTFD	2048:2067	The toxicity of TTFD were also considered as safety for long-term supplementation with indicated doses.
29966293	12	45	theme	histopathological	1870:1886	arg1	data					1888:1891	biochemical and histopathological data	1854:1891	biochemical and histopathological data	1854:1891	According to body composition, biochemical and histopathological data, daily administration of TTFD for over 6 weeks (subacute toxicity) also demonstrated reasonable safety results for long-term and adequate supplementation.
29966293	12	45	theme	histopathological	1870:1886	arg1	administration					1900:1913	daily administration	1894:1913	daily administration of TTFD for over 6 weeks (subacute toxicity)	1894:1958	According to body composition, biochemical and histopathological data, daily administration of TTFD for over 6 weeks (subacute toxicity) also demonstrated reasonable safety results for long-term and adequate supplementation.
29966293	2	46	with	derivative	368:377	arg1	higher					396:401	higher	396:401	higher	396:401	The thiamine tetrahydrofurfuryl disulfide (TTFD) is a derivative of thiamine with higher bioavailability and solubility than thiamine and has been widely applied to health maintenance and disease therapy.
29966293	2	47	dep	higher	396:401	arg1	bioavailability					403:417	bioavailability	403:417	bioavailability	403:417	The thiamine tetrahydrofurfuryl disulfide (TTFD) is a derivative of thiamine with higher bioavailability and solubility than thiamine and has been widely applied to health maintenance and disease therapy.
29966293	2	47	dep	higher	396:401	arg1	solubility					423:432	solubility	423:432	solubility	423:432	The thiamine tetrahydrofurfuryl disulfide (TTFD) is a derivative of thiamine with higher bioavailability and solubility than thiamine and has been widely applied to health maintenance and disease therapy.
29966293	4	48	theme	effective	644:652	arg1	dose					654:657	the effective dose	640:657	the effective dose	640:657	Furthermore, the effective dose of TTFD, beneficial to exercise physiological adaption and performance, still be further validated and the safety evaluation were also an important issue to be considered for potential application.
29966293	2	49	theme	thiamine	382:389	arg1	derivative					368:377	a derivative	366:377	a derivative of thiamine	366:389	The thiamine tetrahydrofurfuryl disulfide (TTFD) is a derivative of thiamine with higher bioavailability and solubility than thiamine and has been widely applied to health maintenance and disease therapy.
29966293	2	49	theme	thiamine	382:389	arg1	disulfide					346:354	The thiamine tetrahydrofurfuryl disulfide	314:354	The thiamine tetrahydrofurfuryl disulfide (TTFD)	314:361	The thiamine tetrahydrofurfuryl disulfide (TTFD) is a derivative of thiamine with higher bioavailability and solubility than thiamine and has been widely applied to health maintenance and disease therapy.
29966293	9	50	theme	Toxicity	1366:1373	arg1	assessments					1375:1385	Toxicity assessments	1366:1385	Toxicity assessments for long-term supplementation	1366:1415	Toxicity assessments for long-term supplementation were also further evaluated for safety consideration.
29966293	12	51	theme	body	1836:1839	arg1	composition					1841:1851	body composition	1836:1851	body composition	1836:1851	According to body composition, biochemical and histopathological data, daily administration of TTFD for over 6 weeks (subacute toxicity) also demonstrated reasonable safety results for long-term and adequate supplementation.
29966293	14	52	theme	adaption	2271:2278	arg1	promotion					2214:2222	the health promotion	2203:2222	not only the health promotion but also improvement of exercise physiological adaption	2194:2278	Furthermore, the TTDF could be applied to not only the health promotion but also improvement of exercise physiological adaption and the TTFD could be further considered as potential ergogenic aids combined with different nutrient strategy.
29966293	14	52	theme	adaption	2271:2278	arg1	improvement					2233:2243	improvement	2233:2243	not only the health promotion but also improvement of exercise physiological adaption	2194:2278	Furthermore, the TTDF could be applied to not only the health promotion but also improvement of exercise physiological adaption and the TTFD could be further considered as potential ergogenic aids combined with different nutrient strategy.
29966293	3	53	theme	physical	526:533	arg1	activities					535:544	Higher physical activities	519:544	Higher physical activities	519:544	Higher physical activities are associated with higher thiamine supplements for efficient energy metabolism.
29966293	14	54	theme	exercise	2248:2255	arg1	adaption					2271:2278	exercise physiological adaption	2248:2278	exercise physiological adaption	2248:2278	Furthermore, the TTDF could be applied to not only the health promotion but also improvement of exercise physiological adaption and the TTFD could be further considered as potential ergogenic aids combined with different nutrient strategy.
29966293	11	55	theme	TTFD	1664:1667	arg1	supplementation					1669:1683	The TTFD supplementation	1660:1683	The TTFD supplementation	1660:1683	The TTFD supplementation significantly mitigated the BUN and CK indexes after extended exercise and elevated the glycogen content in the liver and muscle tissues.
29966293	3	56	theme	thiamine	573:580	arg1	supplements					582:592	higher thiamine supplements	566:592	higher thiamine supplements for efficient energy metabolism	566:624	Higher physical activities are associated with higher thiamine supplements for efficient energy metabolism.
29966293	5	57	dep	ICR	857:859	arg1	Institute					862:870	Institute	862:870	Institute of Cancer Research	862:889	ICR (Institute of Cancer Research) strain mice were allocated as 0, 50, 100, and 500 mg/kg dose groups and administrated by oral gavage consecutively for 6 weeks.
29966293	10	58	theme	lactate	1587:1593	arg1	production					1595:1604	lactate production	1587:1604	lactate production	1587:1604	TTFD supplementation significantly increased the endurance and grip strength and demonstrated beneficial effects on lactate production and clearance rate after an acute exercise challenge.
29966293	7	59	theme	fatigue-associated	1109:1126	arg1	nitrogen					1188:1195	blood urine nitrogen	1176:1195	blood urine nitrogen (BUN)	1176:1201	Various fatigue-associated biochemical variables such as lactate, glucose, blood urine nitrogen (BUN) or creatine kinase (CK), were also assessed.
29966293	7	59	theme	fatigue-associated	1109:1126	arg1	kinase					1215:1220	creatine kinase	1206:1220	creatine kinase (CK)	1206:1225	Various fatigue-associated biochemical variables such as lactate, glucose, blood urine nitrogen (BUN) or creatine kinase (CK), were also assessed.
29966293	7	59	theme	fatigue-associated	1109:1126	arg1	glucose					1167:1173	glucose	1167:1173	glucose	1167:1173	Various fatigue-associated biochemical variables such as lactate, glucose, blood urine nitrogen (BUN) or creatine kinase (CK), were also assessed.
29966293	7	59	theme	fatigue-associated	1109:1126	arg1	variables					1140:1148	Various fatigue-associated biochemical variables	1101:1148	Various fatigue-associated biochemical variables such as lactate, glucose, blood urine nitrogen (BUN) or creatine kinase (CK),	1101:1226	Various fatigue-associated biochemical variables such as lactate, glucose, blood urine nitrogen (BUN) or creatine kinase (CK), were also assessed.
29966293	7	59	theme	fatigue-associated	1109:1126	arg1	lactate					1158:1164	lactate	1158:1164	lactate	1158:1164	Various fatigue-associated biochemical variables such as lactate, glucose, blood urine nitrogen (BUN) or creatine kinase (CK), were also assessed.
29966293	5	60	theme	strain	892:897	arg1	mice					899:902	ICR (Institute of Cancer Research) strain mice	857:902	ICR (Institute of Cancer Research) strain mice	857:902	ICR (Institute of Cancer Research) strain mice were allocated as 0, 50, 100, and 500 mg/kg dose groups and administrated by oral gavage consecutively for 6 weeks.
29966293	5	60	theme	strain	892:897	arg1	groups					953:958	0, 50, 100, and 500 mg/kg dose groups	922:958	0, 50, 100, and 500 mg/kg dose groups	922:958	ICR (Institute of Cancer Research) strain mice were allocated as 0, 50, 100, and 500 mg/kg dose groups and administrated by oral gavage consecutively for 6 weeks.
29966293	4	61	theme	physiological	691:703	arg1	adaption					705:712	exercise physiological adaption	682:712	exercise physiological adaption	682:712	Furthermore, the effective dose of TTFD, beneficial to exercise physiological adaption and performance, still be further validated and the safety evaluation were also an important issue to be considered for potential application.
29966293	3	62	theme	energy	608:613	arg1	metabolism					615:624	efficient energy metabolism	598:624	efficient energy metabolism	598:624	Higher physical activities are associated with higher thiamine supplements for efficient energy metabolism.
29966293	7	63	theme	blood	1176:1180	arg1	nitrogen					1188:1195	blood urine nitrogen	1176:1195	blood urine nitrogen (BUN)	1176:1201	Various fatigue-associated biochemical variables such as lactate, glucose, blood urine nitrogen (BUN) or creatine kinase (CK), were also assessed.
29966293	7	63	theme	blood	1176:1180	arg1	BUN					1198:1200	BUN	1198:1200	BUN	1198:1200	Various fatigue-associated biochemical variables such as lactate, glucose, blood urine nitrogen (BUN) or creatine kinase (CK), were also assessed.
29966293	14	64	theme	health	2207:2212	arg1	promotion					2214:2222	the health promotion	2203:2222	not only the health promotion but also improvement of exercise physiological adaption	2194:2278	Furthermore, the TTDF could be applied to not only the health promotion but also improvement of exercise physiological adaption and the TTFD could be further considered as potential ergogenic aids combined with different nutrient strategy.
29966293	2	65	theme	thiamine	318:325	arg1	derivative					368:377	a derivative	366:377	a derivative of thiamine	366:389	The thiamine tetrahydrofurfuryl disulfide (TTFD) is a derivative of thiamine with higher bioavailability and solubility than thiamine and has been widely applied to health maintenance and disease therapy.
29966293	2	65	theme	thiamine	318:325	arg1	TTFD					357:360	TTFD	357:360	TTFD	357:360	The thiamine tetrahydrofurfuryl disulfide (TTFD) is a derivative of thiamine with higher bioavailability and solubility than thiamine and has been widely applied to health maintenance and disease therapy.
29966293	2	65	theme	thiamine	318:325	arg1	disulfide					346:354	The thiamine tetrahydrofurfuryl disulfide	314:354	The thiamine tetrahydrofurfuryl disulfide (TTFD)	314:361	The thiamine tetrahydrofurfuryl disulfide (TTFD) is a derivative of thiamine with higher bioavailability and solubility than thiamine and has been widely applied to health maintenance and disease therapy.
29966293	9	66	theme	safety	1449:1454	arg1	consideration					1456:1468	safety consideration	1449:1468	safety consideration	1449:1468	Toxicity assessments for long-term supplementation were also further evaluated for safety consideration.
29966293	5	67	theme	Research	882:889	arg1	Institute					862:870	Institute	862:870	Institute of Cancer Research	862:889	ICR (Institute of Cancer Research) strain mice were allocated as 0, 50, 100, and 500 mg/kg dose groups and administrated by oral gavage consecutively for 6 weeks.
29966293	10	68	theme	exercise	1640:1647	arg1	challenge					1649:1657	an acute exercise challenge	1631:1657	an acute exercise challenge	1631:1657	TTFD supplementation significantly increased the endurance and grip strength and demonstrated beneficial effects on lactate production and clearance rate after an acute exercise challenge.
29966293	12	69	theme	safety	1989:1994	arg1	results					1996:2002	reasonable safety results	1978:2002	reasonable safety results for long-term and adequate supplementation	1978:2045	According to body composition, biochemical and histopathological data, daily administration of TTFD for over 6 weeks (subacute toxicity) also demonstrated reasonable safety results for long-term and adequate supplementation.
29966293	11	70	from	content	1782:1788	arg1	tissues					1814:1820	the liver and muscle tissues	1793:1820	the liver and muscle tissues	1793:1820	The TTFD supplementation significantly mitigated the BUN and CK indexes after extended exercise and elevated the glycogen content in the liver and muscle tissues.
29966293	1	71	theme	vitamin	136:142	arg1	B1					144:145	vitamin B1	136:145	vitamin B1	136:145	Thiamine, named as vitamin B1, is an important cofactor for the critical enzymes regarding to glucose metabolism, like transketolase, pyruvate dehydrogenase, and alpha-ketoglutarate dehydrogenase.
29966293	8	72	theme	glycogen	1279:1286	arg1	levels					1252:1257	The levels	1248:1257	The levels of liver and muscle glycogen	1248:1286	The levels of liver and muscle glycogen were measured as an indicator of energy storage at the end of the experiment.
29966293	8	72	theme	glycogen	1279:1286	arg1	indicator					1308:1316	an indicator	1305:1316	an indicator of energy storage	1305:1334	The levels of liver and muscle glycogen were measured as an indicator of energy storage at the end of the experiment.
29966293	14	73	theme	different	2363:2371	arg1	strategy					2382:2389	different nutrient strategy	2363:2389	different nutrient strategy	2363:2389	Furthermore, the TTDF could be applied to not only the health promotion but also improvement of exercise physiological adaption and the TTFD could be further considered as potential ergogenic aids combined with different nutrient strategy.
29966293	0	74	theme	Performance	92:102	arg1	Improvement					104:114	Exercise Performance Improvement	83:114	Exercise Performance Improvement	83:114	The Effects of Thiamine Tetrahydrofurfuryl Disulfide on Physiological Adaption and Exercise Performance Improvement.
29966293	2	75	theme	disease	502:508	arg1	therapy					510:516	disease therapy	502:516	disease therapy	502:516	The thiamine tetrahydrofurfuryl disulfide (TTFD) is a derivative of thiamine with higher bioavailability and solubility than thiamine and has been widely applied to health maintenance and disease therapy.
29966293	0	76	from	Effects	4:10	arg1	Adaption					70:77	Physiological Adaption	56:77	Physiological Adaption	56:77	The Effects of Thiamine Tetrahydrofurfuryl Disulfide on Physiological Adaption and Exercise Performance Improvement.
29966293	0	76	from	Effects	4:10	arg1	Improvement					104:114	Exercise Performance Improvement	83:114	Exercise Performance Improvement	83:114	The Effects of Thiamine Tetrahydrofurfuryl Disulfide on Physiological Adaption and Exercise Performance Improvement.
29966293	7	77	theme	creatine	1206:1213	arg1	CK					1223:1224	CK	1223:1224	CK	1223:1224	Various fatigue-associated biochemical variables such as lactate, glucose, blood urine nitrogen (BUN) or creatine kinase (CK), were also assessed.
29966293	7	77	theme	creatine	1206:1213	arg1	kinase					1215:1220	creatine kinase	1206:1220	creatine kinase (CK)	1206:1225	Various fatigue-associated biochemical variables such as lactate, glucose, blood urine nitrogen (BUN) or creatine kinase (CK), were also assessed.
29966293	13	78	theme	indicated	2135:2143	arg1	doses					2145:2149	indicated doses	2135:2149	indicated doses	2135:2149	The toxicity of TTFD were also considered as safety for long-term supplementation with indicated doses.
29966293	13	79	with	supplementation	2114:2128	arg1	doses					2145:2149	indicated doses	2135:2149	indicated doses	2135:2149	The toxicity of TTFD were also considered as safety for long-term supplementation with indicated doses.
29966293	4	80	theme	important	797:805	arg1	issue					807:811	an important issue	794:811	an important issue	794:811	Furthermore, the effective dose of TTFD, beneficial to exercise physiological adaption and performance, still be further validated and the safety evaluation were also an important issue to be considered for potential application.
29966293	4	80	theme	important	797:805	arg1	evaluation					773:782	the safety evaluation	762:782	the safety evaluation	762:782	Furthermore, the effective dose of TTFD, beneficial to exercise physiological adaption and performance, still be further validated and the safety evaluation were also an important issue to be considered for potential application.
29966293	14	81	theme	ergogenic	2334:2342	arg1	aids					2344:2347	potential ergogenic aids	2324:2347	potential ergogenic aids combined with different nutrient strategy	2324:2389	Furthermore, the TTDF could be applied to not only the health promotion but also improvement of exercise physiological adaption and the TTFD could be further considered as potential ergogenic aids combined with different nutrient strategy.
29966293	14	81	theme	ergogenic	2334:2342	arg1	TTFD					2288:2291	the TTFD	2284:2291	the TTFD	2284:2291	Furthermore, the TTDF could be applied to not only the health promotion but also improvement of exercise physiological adaption and the TTFD could be further considered as potential ergogenic aids combined with different nutrient strategy.
29966293	8	82	theme	storage	1328:1334	arg1	levels					1252:1257	The levels	1248:1257	The levels of liver and muscle glycogen	1248:1286	The levels of liver and muscle glycogen were measured as an indicator of energy storage at the end of the experiment.
29966293	8	82	theme	storage	1328:1334	arg1	indicator					1308:1316	an indicator	1305:1316	an indicator of energy storage	1305:1334	The levels of liver and muscle glycogen were measured as an indicator of energy storage at the end of the experiment.
29966293	0	83	theme	Thiamine	15:22	arg1	Disulfide					43:51	Thiamine Tetrahydrofurfuryl Disulfide	15:51	Thiamine Tetrahydrofurfuryl Disulfide	15:51	The Effects of Thiamine Tetrahydrofurfuryl Disulfide on Physiological Adaption and Exercise Performance Improvement.
29966293	1	84	theme	alpha-ketoglutarate	279:297	arg1	dehydrogenase					299:311	alpha-ketoglutarate dehydrogenase	279:311	alpha-ketoglutarate dehydrogenase	279:311	Thiamine, named as vitamin B1, is an important cofactor for the critical enzymes regarding to glucose metabolism, like transketolase, pyruvate dehydrogenase, and alpha-ketoglutarate dehydrogenase.
29966293	5	85	theme	dose	948:951	arg1	mice					899:902	ICR (Institute of Cancer Research) strain mice	857:902	ICR (Institute of Cancer Research) strain mice	857:902	ICR (Institute of Cancer Research) strain mice were allocated as 0, 50, 100, and 500 mg/kg dose groups and administrated by oral gavage consecutively for 6 weeks.
29966293	5	85	theme	dose	948:951	arg1	groups					953:958	0, 50, 100, and 500 mg/kg dose groups	922:958	0, 50, 100, and 500 mg/kg dose groups	922:958	ICR (Institute of Cancer Research) strain mice were allocated as 0, 50, 100, and 500 mg/kg dose groups and administrated by oral gavage consecutively for 6 weeks.
29966293	0	86	theme	Disulfide	43:51	arg1	Effects					4:10	The Effects	0:10	The Effects of Thiamine Tetrahydrofurfuryl Disulfide on Physiological Adaption and Exercise Performance Improvement.	0:115	The Effects of Thiamine Tetrahydrofurfuryl Disulfide on Physiological Adaption and Exercise Performance Improvement.
29966293	8	87	theme	liver	1262:1266	arg1	levels					1252:1257	The levels	1248:1257	The levels of liver and muscle glycogen	1248:1286	The levels of liver and muscle glycogen were measured as an indicator of energy storage at the end of the experiment.
29966293	8	87	theme	liver	1262:1266	arg1	indicator					1308:1316	an indicator	1305:1316	an indicator of energy storage	1305:1334	The levels of liver and muscle glycogen were measured as an indicator of energy storage at the end of the experiment.
29966293	6	88	theme	grip	1050:1053	arg1	strength					1055:1062	grip strength	1050:1062	grip strength	1050:1062	Physical activities including grip strength and aerobic endurance were measured.
29966293	11	89	theme	CK	1721:1722	arg1	indexes					1724:1730	the BUN and CK indexes	1709:1730	the BUN and CK indexes after extended exercise	1709:1754	The TTFD supplementation significantly mitigated the BUN and CK indexes after extended exercise and elevated the glycogen content in the liver and muscle tissues.
29966293	8	90	theme	experiment	1354:1363	arg1	end					1343:1345	the end	1339:1345	the end of the experiment	1339:1363	The levels of liver and muscle glycogen were measured as an indicator of energy storage at the end of the experiment.
29966293	0	91	theme	Physiological	56:68	arg1	Adaption					70:77	Physiological Adaption	56:77	Physiological Adaption	56:77	The Effects of Thiamine Tetrahydrofurfuryl Disulfide on Physiological Adaption and Exercise Performance Improvement.
29966293	10	92	theme	grip	1534:1537	arg1	strength					1539:1546	the endurance and grip strength	1516:1546	strength	1539:1546	TTFD supplementation significantly increased the endurance and grip strength and demonstrated beneficial effects on lactate production and clearance rate after an acute exercise challenge.
29966293	3	93	theme	Higher	519:524	arg1	activities					535:544	Higher physical activities	519:544	Higher physical activities	519:544	Higher physical activities are associated with higher thiamine supplements for efficient energy metabolism.
29966293	12	94	theme	daily	1894:1898	arg1	data					1888:1891	biochemical and histopathological data	1854:1891	biochemical and histopathological data	1854:1891	According to body composition, biochemical and histopathological data, daily administration of TTFD for over 6 weeks (subacute toxicity) also demonstrated reasonable safety results for long-term and adequate supplementation.
29966293	12	94	theme	daily	1894:1898	arg1	administration					1900:1913	daily administration	1894:1913	daily administration of TTFD for over 6 weeks (subacute toxicity)	1894:1958	According to body composition, biochemical and histopathological data, daily administration of TTFD for over 6 weeks (subacute toxicity) also demonstrated reasonable safety results for long-term and adequate supplementation.
29966293	12	95	theme	biochemical	1854:1864	arg1	data					1888:1891	biochemical and histopathological data	1854:1891	biochemical and histopathological data	1854:1891	According to body composition, biochemical and histopathological data, daily administration of TTFD for over 6 weeks (subacute toxicity) also demonstrated reasonable safety results for long-term and adequate supplementation.
29966293	12	95	theme	biochemical	1854:1864	arg1	administration					1900:1913	daily administration	1894:1913	daily administration of TTFD for over 6 weeks (subacute toxicity)	1894:1958	According to body composition, biochemical and histopathological data, daily administration of TTFD for over 6 weeks (subacute toxicity) also demonstrated reasonable safety results for long-term and adequate supplementation.
29966293	10	96	theme	endurance	1520:1528	arg1	strength					1539:1546	the endurance and grip strength	1516:1546	strength	1539:1546	TTFD supplementation significantly increased the endurance and grip strength and demonstrated beneficial effects on lactate production and clearance rate after an acute exercise challenge.
29966293	4	97	theme	TTFD	662:665	arg1	dose					654:657	the effective dose	640:657	the effective dose	640:657	Furthermore, the effective dose of TTFD, beneficial to exercise physiological adaption and performance, still be further validated and the safety evaluation were also an important issue to be considered for potential application.
29966293	10	98	theme	beneficial	1565:1574	arg1	effects					1576:1582	beneficial effects	1565:1582	beneficial effects on lactate production and clearance rate after an acute exercise challenge	1565:1657	TTFD supplementation significantly increased the endurance and grip strength and demonstrated beneficial effects on lactate production and clearance rate after an acute exercise challenge.
29966293	4	99	theme	potential	834:842	arg1	application					844:854	potential application	834:854	potential application	834:854	Furthermore, the effective dose of TTFD, beneficial to exercise physiological adaption and performance, still be further validated and the safety evaluation were also an important issue to be considered for potential application.
29713152	9	0	dep	22.35	1381:1385	arg1	P=0.000					1408:1414	P=0.000	1408:1414	P=0.000	1408:1414	Coronary heart disease (OR, 1.85; 95% CI: 1.18-2.89; P=0.007), venous thrombosis at admission (OR, 22.35; 95% CI: 6.78-73.60; P=0.000), days between fracture and operation (OR, 1.06; 95% CI: 1.01-1.11; P=0.021), and blood loss (OR, 1.002; 95% CI: 1.000-1.003; P=0.014) were independent risk factors of postoperative DVT.
29713152	9	0	dep	22.35	1381:1385	arg1	%					1390:1390	95% CI: 6.78-73.60	1388:1405	22.35; 95% CI: 6.78-73.60; P=0.000	1381:1414	Coronary heart disease (OR, 1.85; 95% CI: 1.18-2.89; P=0.007), venous thrombosis at admission (OR, 22.35; 95% CI: 6.78-73.60; P=0.000), days between fracture and operation (OR, 1.06; 95% CI: 1.01-1.11; P=0.021), and blood loss (OR, 1.002; 95% CI: 1.000-1.003; P=0.014) were independent risk factors of postoperative DVT.
29713152	0	1	from	thrombosis	10:19	arg1	extremities					40:50	bilateral lower extremities	24:50	bilateral lower extremities	24:50	Deep vein thrombosis in bilateral lower extremities after hip fracture: a retrospective study of 463 patients.
29713152	9	2	theme	independent	1556:1566	arg1	factors					1573:1579	independent risk factors	1556:1579	independent risk factors of postoperative DVT	1556:1600	Coronary heart disease (OR, 1.85; 95% CI: 1.18-2.89; P=0.007), venous thrombosis at admission (OR, 22.35; 95% CI: 6.78-73.60; P=0.000), days between fracture and operation (OR, 1.06; 95% CI: 1.01-1.11; P=0.021), and blood loss (OR, 1.002; 95% CI: 1.000-1.003; P=0.014) were independent risk factors of postoperative DVT.
29713152	9	3	dep	1.06	1459:1462	arg1	P=0.021					1484:1490	P=0.021	1484:1490	P=0.021	1484:1490	Coronary heart disease (OR, 1.85; 95% CI: 1.18-2.89; P=0.007), venous thrombosis at admission (OR, 22.35; 95% CI: 6.78-73.60; P=0.000), days between fracture and operation (OR, 1.06; 95% CI: 1.01-1.11; P=0.021), and blood loss (OR, 1.002; 95% CI: 1.000-1.003; P=0.014) were independent risk factors of postoperative DVT.
29713152	9	3	dep	1.06	1459:1462	arg1	%					1467:1467	95% CI: 1.01-1.11	1465:1481	1.06; 95% CI: 1.01-1.11	1459:1481	Coronary heart disease (OR, 1.85; 95% CI: 1.18-2.89; P=0.007), venous thrombosis at admission (OR, 22.35; 95% CI: 6.78-73.60; P=0.000), days between fracture and operation (OR, 1.06; 95% CI: 1.01-1.11; P=0.021), and blood loss (OR, 1.002; 95% CI: 1.000-1.003; P=0.014) were independent risk factors of postoperative DVT.
29713152	8	4	theme	%	1201:1201	arg1	CI					1203:1204	95% CI	1199:1204	95% CI	1199:1204	Age (odds ratio [OR], 1.03; 95% CI: 1.01-1.04; P=0.002), venous thrombosis at admission (OR, 4.05; 95% CI, 2.30-7.13; P=0.000), and the days between the fracture and the operation (OR, 1.10; 95% CI, 1.02-1.20; P=0.020) were the independent risk factors of preoperative DVT.
29713152	2	5	theme	inclusion	468:476	arg1	criteria					478:485	all the inclusion criteria	460:485	all the inclusion criteria	460:485	PATIENTS AND METHODS We collected the clinical data of 463 patients with lower extremities fractures who presented at Xi'an Honghui Hospital between July 1, 2014, and October 31, 2016 and met all the inclusion criteria.
29713152	10	6	dep	CONCLUSION	1603:1612	arg1	underestimated					1668:1681	underestimated	1668:1681	may be underestimated	1661:1681	CONCLUSION The actual incidence of DVT after hip fracture may be underestimated.
29713152	9	7	theme	venous	1345:1350	arg1	thrombosis					1352:1361	venous thrombosis	1345:1361	venous thrombosis at admission (OR, 22.35; 95% CI: 6.78-73.60; P=0.000), days between fracture and operation (OR, 1.06; 95% CI: 1.01-1.11; P=0.021)	1345:1491	Coronary heart disease (OR, 1.85; 95% CI: 1.18-2.89; P=0.007), venous thrombosis at admission (OR, 22.35; 95% CI: 6.78-73.60; P=0.000), days between fracture and operation (OR, 1.06; 95% CI: 1.01-1.11; P=0.021), and blood loss (OR, 1.002; 95% CI: 1.000-1.003; P=0.014) were independent risk factors of postoperative DVT.
29713152	9	8	theme	risk	1568:1571	arg1	factors					1573:1579	independent risk factors	1556:1579	independent risk factors of postoperative DVT	1556:1600	Coronary heart disease (OR, 1.85; 95% CI: 1.18-2.89; P=0.007), venous thrombosis at admission (OR, 22.35; 95% CI: 6.78-73.60; P=0.000), days between fracture and operation (OR, 1.06; 95% CI: 1.01-1.11; P=0.021), and blood loss (OR, 1.002; 95% CI: 1.000-1.003; P=0.014) were independent risk factors of postoperative DVT.
29713152	4	9	theme	non-thrombosis	636:649	arg1	group					651:655	the thrombosis and non-thrombosis group	617:655	group	651:655	The patients were examined preoperatively and postoperatively and divided into the thrombosis and non-thrombosis group according to the ultrasonographic findings.
29713152	10	10	theme	hip	1648:1650	arg1	fracture					1652:1659	hip fracture	1648:1659	hip fracture	1648:1659	CONCLUSION The actual incidence of DVT after hip fracture may be underestimated.
29713152	1	11	theme	hip	227:229	arg1	fractures					231:239	hip fractures	227:239	hip fractures in both lower extremities	227:265	OBJECTIVE To investigate the incidences of deep vein thrombosis (DVT) before and after operation in inpatients with hip fractures in both lower extremities.
29713152	8	12	theme	risk	1248:1251	arg1	Age					1008:1010	Age	1008:1010	Age (odds ratio [OR], 1.03; 95% CI: 1.01-1.04; P=0.002)	1008:1062	Age (odds ratio [OR], 1.03; 95% CI: 1.01-1.04; P=0.002), venous thrombosis at admission (OR, 4.05; 95% CI, 2.30-7.13; P=0.000), and the days between the fracture and the operation (OR, 1.10; 95% CI, 1.02-1.20; P=0.020) were the independent risk factors of preoperative DVT.
29713152	8	12	theme	risk	1248:1251	arg1	thrombosis					1072:1081	venous thrombosis	1065:1081	venous thrombosis at admission (OR, 4.05; 95% CI, 2.30-7.13; P=0.000), and the days between the fracture	1065:1168	Age (odds ratio [OR], 1.03; 95% CI: 1.01-1.04; P=0.002), venous thrombosis at admission (OR, 4.05; 95% CI, 2.30-7.13; P=0.000), and the days between the fracture and the operation (OR, 1.10; 95% CI, 1.02-1.20; P=0.020) were the independent risk factors of preoperative DVT.
29713152	8	12	theme	risk	1248:1251	arg1	factors					1253:1259	the independent risk factors	1232:1259	the independent risk factors of preoperative DVT	1232:1279	Age (odds ratio [OR], 1.03; 95% CI: 1.01-1.04; P=0.002), venous thrombosis at admission (OR, 4.05; 95% CI, 2.30-7.13; P=0.000), and the days between the fracture and the operation (OR, 1.10; 95% CI, 1.02-1.20; P=0.020) were the independent risk factors of preoperative DVT.
29713152	8	12	theme	risk	1248:1251	arg1	operation					1178:1186	the operation	1174:1186	the operation (OR, 1.10; 95% CI, 1.02-1.20; P=0.020)	1174:1225	Age (odds ratio [OR], 1.03; 95% CI: 1.01-1.04; P=0.002), venous thrombosis at admission (OR, 4.05; 95% CI, 2.30-7.13; P=0.000), and the days between the fracture and the operation (OR, 1.10; 95% CI, 1.02-1.20; P=0.020) were the independent risk factors of preoperative DVT.
29713152	9	13	theme	Coronary	1282:1289	arg1	disease					1297:1303	Coronary heart disease	1282:1303	Coronary heart disease (OR, 1.85; 95% CI: 1.18-2.89; P=0.007)	1282:1342	Coronary heart disease (OR, 1.85; 95% CI: 1.18-2.89; P=0.007), venous thrombosis at admission (OR, 22.35; 95% CI: 6.78-73.60; P=0.000), days between fracture and operation (OR, 1.06; 95% CI: 1.01-1.11; P=0.021), and blood loss (OR, 1.002; 95% CI: 1.000-1.003; P=0.014) were independent risk factors of postoperative DVT.
29713152	9	13	theme	Coronary	1282:1289	arg1	1.85					1310:1313	1.85	1310:1313	1.85; 95% CI: 1.18-2.89	1310:1332	Coronary heart disease (OR, 1.85; 95% CI: 1.18-2.89; P=0.007), venous thrombosis at admission (OR, 22.35; 95% CI: 6.78-73.60; P=0.000), days between fracture and operation (OR, 1.06; 95% CI: 1.01-1.11; P=0.021), and blood loss (OR, 1.002; 95% CI: 1.000-1.003; P=0.014) were independent risk factors of postoperative DVT.
29713152	9	14	theme	blood	1498:1502	arg1	loss					1504:1507	blood loss	1498:1507	blood loss (OR, 1.002; 95% CI: 1.000-1.003; P=0.014)	1498:1549	Coronary heart disease (OR, 1.85; 95% CI: 1.18-2.89; P=0.007), venous thrombosis at admission (OR, 22.35; 95% CI: 6.78-73.60; P=0.000), days between fracture and operation (OR, 1.06; 95% CI: 1.01-1.11; P=0.021), and blood loss (OR, 1.002; 95% CI: 1.000-1.003; P=0.014) were independent risk factors of postoperative DVT.
29713152	11	15	theme	uninjured	1770:1778	arg1	limb					1780:1783	the uninjured limb	1766:1783	the uninjured limb	1766:1783	The incidences of preoperative and postoperative DVTs and the incidence of DVT on the uninjured limb were high.
29713152	0	16	theme	retrospective	74:86	arg1	study					88:92	a retrospective study	72:92	Deep vein thrombosis in bilateral lower extremities after hip fracture: a retrospective study of 463 patients.	0:109	Deep vein thrombosis in bilateral lower extremities after hip fracture: a retrospective study of 463 patients.
29713152	8	17	theme	preoperative	1264:1275	arg1	DVT					1277:1279	preoperative DVT	1264:1279	preoperative DVT	1264:1279	Age (odds ratio [OR], 1.03; 95% CI: 1.01-1.04; P=0.002), venous thrombosis at admission (OR, 4.05; 95% CI, 2.30-7.13; P=0.000), and the days between the fracture and the operation (OR, 1.10; 95% CI, 1.02-1.20; P=0.020) were the independent risk factors of preoperative DVT.
29713152	9	18	theme	heart	1291:1295	arg1	disease					1297:1303	Coronary heart disease	1282:1303	Coronary heart disease (OR, 1.85; 95% CI: 1.18-2.89; P=0.007)	1282:1342	Coronary heart disease (OR, 1.85; 95% CI: 1.18-2.89; P=0.007), venous thrombosis at admission (OR, 22.35; 95% CI: 6.78-73.60; P=0.000), days between fracture and operation (OR, 1.06; 95% CI: 1.01-1.11; P=0.021), and blood loss (OR, 1.002; 95% CI: 1.000-1.003; P=0.014) were independent risk factors of postoperative DVT.
29713152	9	18	theme	heart	1291:1295	arg1	1.85					1310:1313	1.85	1310:1313	1.85; 95% CI: 1.18-2.89	1310:1332	Coronary heart disease (OR, 1.85; 95% CI: 1.18-2.89; P=0.007), venous thrombosis at admission (OR, 22.35; 95% CI: 6.78-73.60; P=0.000), days between fracture and operation (OR, 1.06; 95% CI: 1.01-1.11; P=0.021), and blood loss (OR, 1.002; 95% CI: 1.000-1.003; P=0.014) were independent risk factors of postoperative DVT.
29713152	8	19	dep	4.05	1101:1104	arg1	P=0.000					1126:1132	P=0.000	1126:1132	P=0.000	1126:1132	Age (odds ratio [OR], 1.03; 95% CI: 1.01-1.04; P=0.002), venous thrombosis at admission (OR, 4.05; 95% CI, 2.30-7.13; P=0.000), and the days between the fracture and the operation (OR, 1.10; 95% CI, 1.02-1.20; P=0.020) were the independent risk factors of preoperative DVT.
29713152	8	19	dep	4.05	1101:1104	arg1	%					1109:1109	95% CI	1107:1112	95% CI	1107:1112	Age (odds ratio [OR], 1.03; 95% CI: 1.01-1.04; P=0.002), venous thrombosis at admission (OR, 4.05; 95% CI, 2.30-7.13; P=0.000), and the days between the fracture and the operation (OR, 1.10; 95% CI, 1.02-1.20; P=0.020) were the independent risk factors of preoperative DVT.
29713152	8	20	theme	%	1038:1038	arg1	CI					1040:1041	1.03; 95% CI: 1.01-1.04	1030:1052	1.03; 95% CI: 1.01-1.04	1030:1052	Age (odds ratio [OR], 1.03; 95% CI: 1.01-1.04; P=0.002), venous thrombosis at admission (OR, 4.05; 95% CI, 2.30-7.13; P=0.000), and the days between the fracture and the operation (OR, 1.10; 95% CI, 1.02-1.20; P=0.020) were the independent risk factors of preoperative DVT.
29713152	11	21	theme	preoperative	1702:1713	arg1	DVTs					1733:1736	preoperative and postoperative DVTs	1702:1736	preoperative and postoperative DVTs	1702:1736	The incidences of preoperative and postoperative DVTs and the incidence of DVT on the uninjured limb were high.
29713152	9	22	theme	postoperative	1584:1596	arg1	DVT					1598:1600	postoperative DVT	1584:1600	postoperative DVT	1584:1600	Coronary heart disease (OR, 1.85; 95% CI: 1.18-2.89; P=0.007), venous thrombosis at admission (OR, 22.35; 95% CI: 6.78-73.60; P=0.000), days between fracture and operation (OR, 1.06; 95% CI: 1.01-1.11; P=0.021), and blood loss (OR, 1.002; 95% CI: 1.000-1.003; P=0.014) were independent risk factors of postoperative DVT.
29713152	2	23	theme	extremities	347:357	arg1	fractures					359:367	lower extremities fractures	341:367	lower extremities fractures	341:367	PATIENTS AND METHODS We collected the clinical data of 463 patients with lower extremities fractures who presented at Xi'an Honghui Hospital between July 1, 2014, and October 31, 2016 and met all the inclusion criteria.
29713152	1	24	theme	deep	154:157	arg1	thrombosis					164:173	deep vein thrombosis	154:173	deep vein thrombosis (DVT)	154:179	OBJECTIVE To investigate the incidences of deep vein thrombosis (DVT) before and after operation in inpatients with hip fractures in both lower extremities.
29713152	1	24	theme	deep	154:157	arg1	DVT					176:178	DVT	176:178	DVT	176:178	OBJECTIVE To investigate the incidences of deep vein thrombosis (DVT) before and after operation in inpatients with hip fractures in both lower extremities.
29713152	5	25	theme	central	731:737	arg1	thromboses					762:771	central, peripheral, and mixed thromboses	731:771	central, peripheral, and mixed thromboses	731:771	We divided the DVT cases into central, peripheral, and mixed thromboses.
29713152	6	26	theme	preoperative	799:810	arg1	DVT					812:814	preoperative DVT	799:814	preoperative DVT	799:814	RESULTS The incidence of preoperative DVT was 34.98%, and the prevalence of DVT on the uninjured side was 13.60%.
29713152	5	27	theme	mixed	756:760	arg1	thromboses					762:771	central, peripheral, and mixed thromboses	731:771	central, peripheral, and mixed thromboses	731:771	We divided the DVT cases into central, peripheral, and mixed thromboses.
29713152	1	28	theme	vein	159:162	arg1	thrombosis					164:173	deep vein thrombosis	154:173	deep vein thrombosis (DVT)	154:179	OBJECTIVE To investigate the incidences of deep vein thrombosis (DVT) before and after operation in inpatients with hip fractures in both lower extremities.
29713152	1	28	theme	vein	159:162	arg1	DVT					176:178	DVT	176:178	DVT	176:178	OBJECTIVE To investigate the incidences of deep vein thrombosis (DVT) before and after operation in inpatients with hip fractures in both lower extremities.
29713152	0	29	theme	vein	5:8	arg1	thrombosis					10:19	Deep vein thrombosis	0:19	Deep vein thrombosis in bilateral lower extremities after hip fracture: a retrospective study of 463 patients.	0:109	Deep vein thrombosis in bilateral lower extremities after hip fracture: a retrospective study of 463 patients.
29713152	8	30	theme	DVT	1277:1279	arg1	Age					1008:1010	Age	1008:1010	Age (odds ratio [OR], 1.03; 95% CI: 1.01-1.04; P=0.002)	1008:1062	Age (odds ratio [OR], 1.03; 95% CI: 1.01-1.04; P=0.002), venous thrombosis at admission (OR, 4.05; 95% CI, 2.30-7.13; P=0.000), and the days between the fracture and the operation (OR, 1.10; 95% CI, 1.02-1.20; P=0.020) were the independent risk factors of preoperative DVT.
29713152	8	30	theme	DVT	1277:1279	arg1	thrombosis					1072:1081	venous thrombosis	1065:1081	venous thrombosis at admission (OR, 4.05; 95% CI, 2.30-7.13; P=0.000), and the days between the fracture	1065:1168	Age (odds ratio [OR], 1.03; 95% CI: 1.01-1.04; P=0.002), venous thrombosis at admission (OR, 4.05; 95% CI, 2.30-7.13; P=0.000), and the days between the fracture and the operation (OR, 1.10; 95% CI, 1.02-1.20; P=0.020) were the independent risk factors of preoperative DVT.
29713152	8	30	theme	DVT	1277:1279	arg1	factors					1253:1259	the independent risk factors	1232:1259	the independent risk factors of preoperative DVT	1232:1279	Age (odds ratio [OR], 1.03; 95% CI: 1.01-1.04; P=0.002), venous thrombosis at admission (OR, 4.05; 95% CI, 2.30-7.13; P=0.000), and the days between the fracture and the operation (OR, 1.10; 95% CI, 1.02-1.20; P=0.020) were the independent risk factors of preoperative DVT.
29713152	8	30	theme	DVT	1277:1279	arg1	operation					1178:1186	the operation	1174:1186	the operation (OR, 1.10; 95% CI, 1.02-1.20; P=0.020)	1174:1225	Age (odds ratio [OR], 1.03; 95% CI: 1.01-1.04; P=0.002), venous thrombosis at admission (OR, 4.05; 95% CI, 2.30-7.13; P=0.000), and the days between the fracture and the operation (OR, 1.10; 95% CI, 1.02-1.20; P=0.020) were the independent risk factors of preoperative DVT.
29713152	1	31	theme	thrombosis	164:173	arg1	incidences					140:149	the incidences	136:149	the incidences of deep vein thrombosis (DVT) before and after operation in inpatients with hip fractures in both lower extremities	136:265	OBJECTIVE To investigate the incidences of deep vein thrombosis (DVT) before and after operation in inpatients with hip fractures in both lower extremities.
29713152	0	32	theme	Deep	0:3	arg1	thrombosis					10:19	Deep vein thrombosis	0:19	Deep vein thrombosis in bilateral lower extremities after hip fracture: a retrospective study of 463 patients.	0:109	Deep vein thrombosis in bilateral lower extremities after hip fracture: a retrospective study of 463 patients.
29713152	11	33	from	incidences	1688:1697	arg1	limb					1780:1783	the uninjured limb	1766:1783	the uninjured limb	1766:1783	The incidences of preoperative and postoperative DVTs and the incidence of DVT on the uninjured limb were high.
29713152	8	34	dep	ratio	1018:1022	arg1	CI					1040:1041	1.03; 95% CI: 1.01-1.04	1030:1052	1.03; 95% CI: 1.01-1.04	1030:1052	Age (odds ratio [OR], 1.03; 95% CI: 1.01-1.04; P=0.002), venous thrombosis at admission (OR, 4.05; 95% CI, 2.30-7.13; P=0.000), and the days between the fracture and the operation (OR, 1.10; 95% CI, 1.02-1.20; P=0.020) were the independent risk factors of preoperative DVT.
29713152	8	34	dep	ratio	1018:1022	arg1	P=0.002					1055:1061	P=0.002	1055:1061	P=0.002	1055:1061	Age (odds ratio [OR], 1.03; 95% CI: 1.01-1.04; P=0.002), venous thrombosis at admission (OR, 4.05; 95% CI, 2.30-7.13; P=0.000), and the days between the fracture and the operation (OR, 1.10; 95% CI, 1.02-1.20; P=0.020) were the independent risk factors of preoperative DVT.
29713152	11	35	theme	incidence	1746:1754	arg1	high					1790:1793	high	1790:1793	high	1790:1793	The incidences of preoperative and postoperative DVTs and the incidence of DVT on the uninjured limb were high.
29713152	11	35	theme	incidence	1746:1754	arg1	incidences					1688:1697	The incidences	1684:1697	The incidences of preoperative and postoperative DVTs and the incidence of DVT on the uninjured limb	1684:1783	The incidences of preoperative and postoperative DVTs and the incidence of DVT on the uninjured limb were high.
29713152	2	36	theme	lower	341:345	arg1	fractures					359:367	lower extremities fractures	341:367	lower extremities fractures	341:367	PATIENTS AND METHODS We collected the clinical data of 463 patients with lower extremities fractures who presented at Xi'an Honghui Hospital between July 1, 2014, and October 31, 2016 and met all the inclusion criteria.
29713152	11	37	theme	postoperative	1719:1731	arg1	DVTs					1733:1736	preoperative and postoperative DVTs	1702:1736	preoperative and postoperative DVTs	1702:1736	The incidences of preoperative and postoperative DVTs and the incidence of DVT on the uninjured limb were high.
29713152	1	38	theme	lower	249:253	arg1	extremities					255:265	both lower extremities	244:265	both lower extremities	244:265	OBJECTIVE To investigate the incidences of deep vein thrombosis (DVT) before and after operation in inpatients with hip fractures in both lower extremities.
29713152	1	39	from	fractures	231:239	arg1	extremities					255:265	both lower extremities	244:265	both lower extremities	244:265	OBJECTIVE To investigate the incidences of deep vein thrombosis (DVT) before and after operation in inpatients with hip fractures in both lower extremities.
29713152	11	40	theme	DVTs	1733:1736	arg1	high					1790:1793	high	1790:1793	high	1790:1793	The incidences of preoperative and postoperative DVTs and the incidence of DVT on the uninjured limb were high.
29713152	11	40	theme	DVTs	1733:1736	arg1	incidences					1688:1697	The incidences	1684:1697	The incidences of preoperative and postoperative DVTs and the incidence of DVT on the uninjured limb	1684:1783	The incidences of preoperative and postoperative DVTs and the incidence of DVT on the uninjured limb were high.
29713152	9	41	from	admission	1366:1374	arg1	disease					1297:1303	Coronary heart disease	1282:1303	Coronary heart disease (OR, 1.85; 95% CI: 1.18-2.89; P=0.007)	1282:1342	Coronary heart disease (OR, 1.85; 95% CI: 1.18-2.89; P=0.007), venous thrombosis at admission (OR, 22.35; 95% CI: 6.78-73.60; P=0.000), days between fracture and operation (OR, 1.06; 95% CI: 1.01-1.11; P=0.021), and blood loss (OR, 1.002; 95% CI: 1.000-1.003; P=0.014) were independent risk factors of postoperative DVT.
29713152	9	41	from	admission	1366:1374	arg1	thrombosis					1352:1361	venous thrombosis	1345:1361	venous thrombosis at admission (OR, 22.35; 95% CI: 6.78-73.60; P=0.000), days between fracture and operation (OR, 1.06; 95% CI: 1.01-1.11; P=0.021)	1345:1491	Coronary heart disease (OR, 1.85; 95% CI: 1.18-2.89; P=0.007), venous thrombosis at admission (OR, 22.35; 95% CI: 6.78-73.60; P=0.000), days between fracture and operation (OR, 1.06; 95% CI: 1.01-1.11; P=0.021), and blood loss (OR, 1.002; 95% CI: 1.000-1.003; P=0.014) were independent risk factors of postoperative DVT.
29713152	9	41	from	admission	1366:1374	arg1	loss					1504:1507	blood loss	1498:1507	blood loss (OR, 1.002; 95% CI: 1.000-1.003; P=0.014)	1498:1549	Coronary heart disease (OR, 1.85; 95% CI: 1.18-2.89; P=0.007), venous thrombosis at admission (OR, 22.35; 95% CI: 6.78-73.60; P=0.000), days between fracture and operation (OR, 1.06; 95% CI: 1.01-1.11; P=0.021), and blood loss (OR, 1.002; 95% CI: 1.000-1.003; P=0.014) were independent risk factors of postoperative DVT.
29713152	9	41	from	admission	1366:1374	arg1	1.85					1310:1313	1.85	1310:1313	1.85; 95% CI: 1.18-2.89	1310:1332	Coronary heart disease (OR, 1.85; 95% CI: 1.18-2.89; P=0.007), venous thrombosis at admission (OR, 22.35; 95% CI: 6.78-73.60; P=0.000), days between fracture and operation (OR, 1.06; 95% CI: 1.01-1.11; P=0.021), and blood loss (OR, 1.002; 95% CI: 1.000-1.003; P=0.014) were independent risk factors of postoperative DVT.
29713152	0	42	theme	patients	101:108	arg1	study					88:92	a retrospective study	72:92	Deep vein thrombosis in bilateral lower extremities after hip fracture: a retrospective study of 463 patients.	0:109	Deep vein thrombosis in bilateral lower extremities after hip fracture: a retrospective study of 463 patients.
29713152	3	43	theme	Doppler	488:494	arg1	ultrasonography					496:510	Doppler ultrasonography	488:510	Doppler ultrasonography	488:510	Doppler ultrasonography was used to diagnose DVT.
29713152	6	44	theme	DVT	850:852	arg1	%					885:885	13.60%	880:885	13.60%	880:885	RESULTS The incidence of preoperative DVT was 34.98%, and the prevalence of DVT on the uninjured side was 13.60%.
29713152	6	44	theme	DVT	850:852	arg1	prevalence					836:845	the prevalence	832:845	the prevalence of DVT on the uninjured side	832:874	RESULTS The incidence of preoperative DVT was 34.98%, and the prevalence of DVT on the uninjured side was 13.60%.
29713152	9	45	dep	1.85	1310:1313	arg1	P=0.007					1335:1341	P=0.007	1335:1341	P=0.007	1335:1341	Coronary heart disease (OR, 1.85; 95% CI: 1.18-2.89; P=0.007), venous thrombosis at admission (OR, 22.35; 95% CI: 6.78-73.60; P=0.000), days between fracture and operation (OR, 1.06; 95% CI: 1.01-1.11; P=0.021), and blood loss (OR, 1.002; 95% CI: 1.000-1.003; P=0.014) were independent risk factors of postoperative DVT.
29713152	9	45	dep	1.85	1310:1313	arg1	%					1318:1318	95% CI: 1.18-2.89	1316:1332	1.85; 95% CI: 1.18-2.89	1310:1332	Coronary heart disease (OR, 1.85; 95% CI: 1.18-2.89; P=0.007), venous thrombosis at admission (OR, 22.35; 95% CI: 6.78-73.60; P=0.000), days between fracture and operation (OR, 1.06; 95% CI: 1.01-1.11; P=0.021), and blood loss (OR, 1.002; 95% CI: 1.000-1.003; P=0.014) were independent risk factors of postoperative DVT.
29713152	0	46	theme	lower	34:38	arg1	extremities					40:50	bilateral lower extremities	24:50	bilateral lower extremities	24:50	Deep vein thrombosis in bilateral lower extremities after hip fracture: a retrospective study of 463 patients.
29713152	2	47	dep	PATIENTS	268:275	arg1	We					289:290	We	289:290	We	289:290	PATIENTS AND METHODS We collected the clinical data of 463 patients with lower extremities fractures who presented at Xi'an Honghui Hospital between July 1, 2014, and October 31, 2016 and met all the inclusion criteria.
29713152	7	48	theme	DVT	970:972	arg1	prevalence					956:965	the prevalence	952:965	the prevalence of DVT on the uninjured side	952:994	This composition ratio increased to 57.23% postoperatively, and the prevalence of DVT on the uninjured side was 25.05%.
29713152	7	48	theme	DVT	970:972	arg1	%					1005:1005	25.05%	1000:1005	25.05%	1000:1005	This composition ratio increased to 57.23% postoperatively, and the prevalence of DVT on the uninjured side was 25.05%.
29713152	7	49	theme	uninjured	981:989	arg1	side					991:994	the uninjured side	977:994	the uninjured side	977:994	This composition ratio increased to 57.23% postoperatively, and the prevalence of DVT on the uninjured side was 25.05%.
29713152	0	50	theme	bilateral	24:32	arg1	extremities					40:50	bilateral lower extremities	24:50	bilateral lower extremities	24:50	Deep vein thrombosis in bilateral lower extremities after hip fracture: a retrospective study of 463 patients.
29713152	6	51	theme	DVT	812:814	arg1	incidence					786:794	The incidence	782:794	RESULTS The incidence of preoperative DVT	774:814	RESULTS The incidence of preoperative DVT was 34.98%, and the prevalence of DVT on the uninjured side was 13.60%.
29713152	5	52	theme	peripheral	740:749	arg1	thromboses					762:771	central, peripheral, and mixed thromboses	731:771	central, peripheral, and mixed thromboses	731:771	We divided the DVT cases into central, peripheral, and mixed thromboses.
29713152	9	53	dep	admission	1366:1374	arg1	22.35					1381:1385	22.35	1381:1385	22.35	1381:1385	Coronary heart disease (OR, 1.85; 95% CI: 1.18-2.89; P=0.007), venous thrombosis at admission (OR, 22.35; 95% CI: 6.78-73.60; P=0.000), days between fracture and operation (OR, 1.06; 95% CI: 1.01-1.11; P=0.021), and blood loss (OR, 1.002; 95% CI: 1.000-1.003; P=0.014) were independent risk factors of postoperative DVT.
29713152	5	54	theme	DVT	716:718	arg1	cases					720:724	the DVT cases	712:724	the DVT cases	712:724	We divided the DVT cases into central, peripheral, and mixed thromboses.
29713152	8	55	from	admission	1086:1094	arg1	Age					1008:1010	Age	1008:1010	Age (odds ratio [OR], 1.03; 95% CI: 1.01-1.04; P=0.002)	1008:1062	Age (odds ratio [OR], 1.03; 95% CI: 1.01-1.04; P=0.002), venous thrombosis at admission (OR, 4.05; 95% CI, 2.30-7.13; P=0.000), and the days between the fracture and the operation (OR, 1.10; 95% CI, 1.02-1.20; P=0.020) were the independent risk factors of preoperative DVT.
29713152	8	55	from	admission	1086:1094	arg1	thrombosis					1072:1081	venous thrombosis	1065:1081	venous thrombosis at admission (OR, 4.05; 95% CI, 2.30-7.13; P=0.000), and the days between the fracture	1065:1168	Age (odds ratio [OR], 1.03; 95% CI: 1.01-1.04; P=0.002), venous thrombosis at admission (OR, 4.05; 95% CI, 2.30-7.13; P=0.000), and the days between the fracture and the operation (OR, 1.10; 95% CI, 1.02-1.20; P=0.020) were the independent risk factors of preoperative DVT.
29713152	8	55	from	admission	1086:1094	arg1	OR					1189:1190	OR	1189:1190	OR	1189:1190	Age (odds ratio [OR], 1.03; 95% CI: 1.01-1.04; P=0.002), venous thrombosis at admission (OR, 4.05; 95% CI, 2.30-7.13; P=0.000), and the days between the fracture and the operation (OR, 1.10; 95% CI, 1.02-1.20; P=0.020) were the independent risk factors of preoperative DVT.
29713152	8	55	from	admission	1086:1094	arg1	ratio					1018:1022	odds ratio	1013:1022	odds ratio [OR	1013:1026	Age (odds ratio [OR], 1.03; 95% CI: 1.01-1.04; P=0.002), venous thrombosis at admission (OR, 4.05; 95% CI, 2.30-7.13; P=0.000), and the days between the fracture and the operation (OR, 1.10; 95% CI, 1.02-1.20; P=0.020) were the independent risk factors of preoperative DVT.
29713152	8	55	from	admission	1086:1094	arg1	factors					1253:1259	the independent risk factors	1232:1259	the independent risk factors of preoperative DVT	1232:1279	Age (odds ratio [OR], 1.03; 95% CI: 1.01-1.04; P=0.002), venous thrombosis at admission (OR, 4.05; 95% CI, 2.30-7.13; P=0.000), and the days between the fracture and the operation (OR, 1.10; 95% CI, 1.02-1.20; P=0.020) were the independent risk factors of preoperative DVT.
29713152	8	55	from	admission	1086:1094	arg1	operation					1178:1186	the operation	1174:1186	the operation (OR, 1.10; 95% CI, 1.02-1.20; P=0.020)	1174:1225	Age (odds ratio [OR], 1.03; 95% CI: 1.01-1.04; P=0.002), venous thrombosis at admission (OR, 4.05; 95% CI, 2.30-7.13; P=0.000), and the days between the fracture and the operation (OR, 1.10; 95% CI, 1.02-1.20; P=0.020) were the independent risk factors of preoperative DVT.
29713152	6	56	dep	RESULTS	774:780	arg1	incidence					786:794	The incidence	782:794	RESULTS The incidence of preoperative DVT	774:814	RESULTS The incidence of preoperative DVT was 34.98%, and the prevalence of DVT on the uninjured side was 13.60%.
29713152	6	57	theme	uninjured	861:869	arg1	side					871:874	the uninjured side	857:874	the uninjured side	857:874	RESULTS The incidence of preoperative DVT was 34.98%, and the prevalence of DVT on the uninjured side was 13.60%.
29713152	3	58	used	used	516:519	arg2	ultrasonography					496:510	Doppler ultrasonography	488:510	Doppler ultrasonography	488:510	Doppler ultrasonography was used to diagnose DVT.
29713152	9	59	dep	loss	1504:1507	arg1	1.002					1514:1518	1.002	1514:1518	1.002	1514:1518	Coronary heart disease (OR, 1.85; 95% CI: 1.18-2.89; P=0.007), venous thrombosis at admission (OR, 22.35; 95% CI: 6.78-73.60; P=0.000), days between fracture and operation (OR, 1.06; 95% CI: 1.01-1.11; P=0.021), and blood loss (OR, 1.002; 95% CI: 1.000-1.003; P=0.014) were independent risk factors of postoperative DVT.
29713152	2	60	theme	clinical	306:313	arg1	data					315:318	the clinical data	302:318	the clinical data of 463 patients with lower extremities fractures who presented at Xi'an Honghui Hospital between July 1, 2014, and October 31, 2016 and met all the inclusion criteria	302:485	PATIENTS AND METHODS We collected the clinical data of 463 patients with lower extremities fractures who presented at Xi'an Honghui Hospital between July 1, 2014, and October 31, 2016 and met all the inclusion criteria.
29713152	8	61	from	days	1144:1147	arg1	Age					1008:1010	Age	1008:1010	Age (odds ratio [OR], 1.03; 95% CI: 1.01-1.04; P=0.002)	1008:1062	Age (odds ratio [OR], 1.03; 95% CI: 1.01-1.04; P=0.002), venous thrombosis at admission (OR, 4.05; 95% CI, 2.30-7.13; P=0.000), and the days between the fracture and the operation (OR, 1.10; 95% CI, 1.02-1.20; P=0.020) were the independent risk factors of preoperative DVT.
29713152	8	61	from	days	1144:1147	arg1	thrombosis					1072:1081	venous thrombosis	1065:1081	venous thrombosis at admission (OR, 4.05; 95% CI, 2.30-7.13; P=0.000), and the days between the fracture	1065:1168	Age (odds ratio [OR], 1.03; 95% CI: 1.01-1.04; P=0.002), venous thrombosis at admission (OR, 4.05; 95% CI, 2.30-7.13; P=0.000), and the days between the fracture and the operation (OR, 1.10; 95% CI, 1.02-1.20; P=0.020) were the independent risk factors of preoperative DVT.
29713152	8	61	from	days	1144:1147	arg1	OR					1189:1190	OR	1189:1190	OR	1189:1190	Age (odds ratio [OR], 1.03; 95% CI: 1.01-1.04; P=0.002), venous thrombosis at admission (OR, 4.05; 95% CI, 2.30-7.13; P=0.000), and the days between the fracture and the operation (OR, 1.10; 95% CI, 1.02-1.20; P=0.020) were the independent risk factors of preoperative DVT.
29713152	8	61	from	days	1144:1147	arg1	ratio					1018:1022	odds ratio	1013:1022	odds ratio [OR	1013:1026	Age (odds ratio [OR], 1.03; 95% CI: 1.01-1.04; P=0.002), venous thrombosis at admission (OR, 4.05; 95% CI, 2.30-7.13; P=0.000), and the days between the fracture and the operation (OR, 1.10; 95% CI, 1.02-1.20; P=0.020) were the independent risk factors of preoperative DVT.
29713152	8	61	from	days	1144:1147	arg1	factors					1253:1259	the independent risk factors	1232:1259	the independent risk factors of preoperative DVT	1232:1279	Age (odds ratio [OR], 1.03; 95% CI: 1.01-1.04; P=0.002), venous thrombosis at admission (OR, 4.05; 95% CI, 2.30-7.13; P=0.000), and the days between the fracture and the operation (OR, 1.10; 95% CI, 1.02-1.20; P=0.020) were the independent risk factors of preoperative DVT.
29713152	8	61	from	days	1144:1147	arg1	operation					1178:1186	the operation	1174:1186	the operation (OR, 1.10; 95% CI, 1.02-1.20; P=0.020)	1174:1225	Age (odds ratio [OR], 1.03; 95% CI: 1.01-1.04; P=0.002), venous thrombosis at admission (OR, 4.05; 95% CI, 2.30-7.13; P=0.000), and the days between the fracture and the operation (OR, 1.10; 95% CI, 1.02-1.20; P=0.020) were the independent risk factors of preoperative DVT.
29713152	6	62	from	prevalence	836:845	arg1	side					871:874	the uninjured side	857:874	the uninjured side	857:874	RESULTS The incidence of preoperative DVT was 34.98%, and the prevalence of DVT on the uninjured side was 13.60%.
29713152	8	63	theme	odds	1013:1016	arg1	Age					1008:1010	Age	1008:1010	Age (odds ratio [OR], 1.03; 95% CI: 1.01-1.04; P=0.002)	1008:1062	Age (odds ratio [OR], 1.03; 95% CI: 1.01-1.04; P=0.002), venous thrombosis at admission (OR, 4.05; 95% CI, 2.30-7.13; P=0.000), and the days between the fracture and the operation (OR, 1.10; 95% CI, 1.02-1.20; P=0.020) were the independent risk factors of preoperative DVT.
29713152	8	63	theme	odds	1013:1016	arg1	ratio					1018:1022	odds ratio	1013:1022	odds ratio [OR	1013:1026	Age (odds ratio [OR], 1.03; 95% CI: 1.01-1.04; P=0.002), venous thrombosis at admission (OR, 4.05; 95% CI, 2.30-7.13; P=0.000), and the days between the fracture and the operation (OR, 1.10; 95% CI, 1.02-1.20; P=0.020) were the independent risk factors of preoperative DVT.
29713152	9	64	theme	DVT	1598:1600	arg1	factors					1573:1579	independent risk factors	1556:1579	independent risk factors of postoperative DVT	1556:1600	Coronary heart disease (OR, 1.85; 95% CI: 1.18-2.89; P=0.007), venous thrombosis at admission (OR, 22.35; 95% CI: 6.78-73.60; P=0.000), days between fracture and operation (OR, 1.06; 95% CI: 1.01-1.11; P=0.021), and blood loss (OR, 1.002; 95% CI: 1.000-1.003; P=0.014) were independent risk factors of postoperative DVT.
29713152	10	65	theme	DVT	1638:1640	arg1	incidence					1625:1633	The actual incidence	1614:1633	The actual incidence of DVT after hip fracture	1614:1659	CONCLUSION The actual incidence of DVT after hip fracture may be underestimated.
29713152	11	66	theme	DVT	1759:1761	arg1	DVTs					1733:1736	preoperative and postoperative DVTs	1702:1736	preoperative and postoperative DVTs	1702:1736	The incidences of preoperative and postoperative DVTs and the incidence of DVT on the uninjured limb were high.
29713152	11	66	theme	DVT	1759:1761	arg1	incidence					1746:1754	the incidence	1742:1754	the incidence of DVT	1742:1761	The incidences of preoperative and postoperative DVTs and the incidence of DVT on the uninjured limb were high.
29713152	4	67	theme	ultrasonographic	674:689	arg1	findings					691:698	the ultrasonographic findings	670:698	the ultrasonographic findings	670:698	The patients were examined preoperatively and postoperatively and divided into the thrombosis and non-thrombosis group according to the ultrasonographic findings.
29713152	9	68	dep	1.002	1514:1518	arg1	1.000-1.003					1529:1539	1.000-1.003	1529:1539	1.000-1.003	1529:1539	Coronary heart disease (OR, 1.85; 95% CI: 1.18-2.89; P=0.007), venous thrombosis at admission (OR, 22.35; 95% CI: 6.78-73.60; P=0.000), days between fracture and operation (OR, 1.06; 95% CI: 1.01-1.11; P=0.021), and blood loss (OR, 1.002; 95% CI: 1.000-1.003; P=0.014) were independent risk factors of postoperative DVT.
29713152	9	68	dep	1.002	1514:1518	arg1	P=0.014					1542:1548	P=0.014	1542:1548	1.002; 95% CI: 1.000-1.003; P=0.014	1514:1548	Coronary heart disease (OR, 1.85; 95% CI: 1.18-2.89; P=0.007), venous thrombosis at admission (OR, 22.35; 95% CI: 6.78-73.60; P=0.000), days between fracture and operation (OR, 1.06; 95% CI: 1.01-1.11; P=0.021), and blood loss (OR, 1.002; 95% CI: 1.000-1.003; P=0.014) were independent risk factors of postoperative DVT.
29713152	9	68	dep	1.002	1514:1518	arg1	%					1523:1523	95% CI	1521:1526	1.002; 95% CI: 1.000-1.003; P=0.014	1514:1548	Coronary heart disease (OR, 1.85; 95% CI: 1.18-2.89; P=0.007), venous thrombosis at admission (OR, 22.35; 95% CI: 6.78-73.60; P=0.000), days between fracture and operation (OR, 1.06; 95% CI: 1.01-1.11; P=0.021), and blood loss (OR, 1.002; 95% CI: 1.000-1.003; P=0.014) were independent risk factors of postoperative DVT.
29713152	1	69	from	operation	198:206	arg1	inpatients					211:220	inpatients	211:220	inpatients with hip fractures in both lower extremities	211:265	OBJECTIVE To investigate the incidences of deep vein thrombosis (DVT) before and after operation in inpatients with hip fractures in both lower extremities.
29713152	0	70	dep	thrombosis	10:19	arg1	study					88:92	a retrospective study	72:92	Deep vein thrombosis in bilateral lower extremities after hip fracture: a retrospective study of 463 patients.	0:109	Deep vein thrombosis in bilateral lower extremities after hip fracture: a retrospective study of 463 patients.
29713152	4	71	theme	thrombosis	621:630	arg1	group					651:655	the thrombosis and non-thrombosis group	617:655	group	651:655	The patients were examined preoperatively and postoperatively and divided into the thrombosis and non-thrombosis group according to the ultrasonographic findings.
29713152	8	72	theme	venous	1065:1070	arg1	Age					1008:1010	Age	1008:1010	Age (odds ratio [OR], 1.03; 95% CI: 1.01-1.04; P=0.002)	1008:1062	Age (odds ratio [OR], 1.03; 95% CI: 1.01-1.04; P=0.002), venous thrombosis at admission (OR, 4.05; 95% CI, 2.30-7.13; P=0.000), and the days between the fracture and the operation (OR, 1.10; 95% CI, 1.02-1.20; P=0.020) were the independent risk factors of preoperative DVT.
29713152	8	72	theme	venous	1065:1070	arg1	thrombosis					1072:1081	venous thrombosis	1065:1081	venous thrombosis at admission (OR, 4.05; 95% CI, 2.30-7.13; P=0.000), and the days between the fracture	1065:1168	Age (odds ratio [OR], 1.03; 95% CI: 1.01-1.04; P=0.002), venous thrombosis at admission (OR, 4.05; 95% CI, 2.30-7.13; P=0.000), and the days between the fracture and the operation (OR, 1.10; 95% CI, 1.02-1.20; P=0.020) were the independent risk factors of preoperative DVT.
29713152	8	72	theme	venous	1065:1070	arg1	factors					1253:1259	the independent risk factors	1232:1259	the independent risk factors of preoperative DVT	1232:1279	Age (odds ratio [OR], 1.03; 95% CI: 1.01-1.04; P=0.002), venous thrombosis at admission (OR, 4.05; 95% CI, 2.30-7.13; P=0.000), and the days between the fracture and the operation (OR, 1.10; 95% CI, 1.02-1.20; P=0.020) were the independent risk factors of preoperative DVT.
29713152	8	72	theme	venous	1065:1070	arg1	operation					1178:1186	the operation	1174:1186	the operation (OR, 1.10; 95% CI, 1.02-1.20; P=0.020)	1174:1225	Age (odds ratio [OR], 1.03; 95% CI: 1.01-1.04; P=0.002), venous thrombosis at admission (OR, 4.05; 95% CI, 2.30-7.13; P=0.000), and the days between the fracture and the operation (OR, 1.10; 95% CI, 1.02-1.20; P=0.020) were the independent risk factors of preoperative DVT.
29713152	8	73	theme	[OR	1024:1026	arg1	Age					1008:1010	Age	1008:1010	Age (odds ratio [OR], 1.03; 95% CI: 1.01-1.04; P=0.002)	1008:1062	Age (odds ratio [OR], 1.03; 95% CI: 1.01-1.04; P=0.002), venous thrombosis at admission (OR, 4.05; 95% CI, 2.30-7.13; P=0.000), and the days between the fracture and the operation (OR, 1.10; 95% CI, 1.02-1.20; P=0.020) were the independent risk factors of preoperative DVT.
29713152	8	73	theme	[OR	1024:1026	arg1	ratio					1018:1022	odds ratio	1013:1022	odds ratio [OR	1013:1026	Age (odds ratio [OR], 1.03; 95% CI: 1.01-1.04; P=0.002), venous thrombosis at admission (OR, 4.05; 95% CI, 2.30-7.13; P=0.000), and the days between the fracture and the operation (OR, 1.10; 95% CI, 1.02-1.20; P=0.020) were the independent risk factors of preoperative DVT.
29713152	2	74	with	patients	327:334	arg1	fractures					359:367	lower extremities fractures	341:367	lower extremities fractures	341:367	PATIENTS AND METHODS We collected the clinical data of 463 patients with lower extremities fractures who presented at Xi'an Honghui Hospital between July 1, 2014, and October 31, 2016 and met all the inclusion criteria.
29713152	1	75	with	inpatients	211:220	arg1	fractures					231:239	hip fractures	227:239	hip fractures in both lower extremities	227:265	OBJECTIVE To investigate the incidences of deep vein thrombosis (DVT) before and after operation in inpatients with hip fractures in both lower extremities.
29713152	8	76	dep	OR	1189:1190	arg1	1.10					1193:1196	1.10	1193:1196	1.10	1193:1196	Age (odds ratio [OR], 1.03; 95% CI: 1.01-1.04; P=0.002), venous thrombosis at admission (OR, 4.05; 95% CI, 2.30-7.13; P=0.000), and the days between the fracture and the operation (OR, 1.10; 95% CI, 1.02-1.20; P=0.020) were the independent risk factors of preoperative DVT.
29713152	8	76	dep	OR	1189:1190	arg1	1.02-1.20					1207:1215	1.02-1.20	1207:1215	1.02-1.20	1207:1215	Age (odds ratio [OR], 1.03; 95% CI: 1.01-1.04; P=0.002), venous thrombosis at admission (OR, 4.05; 95% CI, 2.30-7.13; P=0.000), and the days between the fracture and the operation (OR, 1.10; 95% CI, 1.02-1.20; P=0.020) were the independent risk factors of preoperative DVT.
29713152	8	76	dep	OR	1189:1190	arg1	CI					1203:1204	95% CI	1199:1204	95% CI	1199:1204	Age (odds ratio [OR], 1.03; 95% CI: 1.01-1.04; P=0.002), venous thrombosis at admission (OR, 4.05; 95% CI, 2.30-7.13; P=0.000), and the days between the fracture and the operation (OR, 1.10; 95% CI, 1.02-1.20; P=0.020) were the independent risk factors of preoperative DVT.
29713152	8	76	dep	OR	1189:1190	arg1	P=0.020					1218:1224	P=0.020	1218:1224	P=0.020	1218:1224	Age (odds ratio [OR], 1.03; 95% CI: 1.01-1.04; P=0.002), venous thrombosis at admission (OR, 4.05; 95% CI, 2.30-7.13; P=0.000), and the days between the fracture and the operation (OR, 1.10; 95% CI, 1.02-1.20; P=0.020) were the independent risk factors of preoperative DVT.
29713152	10	77	theme	actual	1618:1623	arg1	incidence					1625:1633	The actual incidence	1614:1633	The actual incidence of DVT after hip fracture	1614:1659	CONCLUSION The actual incidence of DVT after hip fracture may be underestimated.
29713152	7	78	from	prevalence	956:965	arg1	side					991:994	the uninjured side	977:994	the uninjured side	977:994	This composition ratio increased to 57.23% postoperatively, and the prevalence of DVT on the uninjured side was 25.05%.
29713152	0	79	theme	hip	58:60	arg1	fracture					62:69	hip fracture	58:69	hip fracture	58:69	Deep vein thrombosis in bilateral lower extremities after hip fracture: a retrospective study of 463 patients.
29713152	8	80	theme	independent	1236:1246	arg1	Age					1008:1010	Age	1008:1010	Age (odds ratio [OR], 1.03; 95% CI: 1.01-1.04; P=0.002)	1008:1062	Age (odds ratio [OR], 1.03; 95% CI: 1.01-1.04; P=0.002), venous thrombosis at admission (OR, 4.05; 95% CI, 2.30-7.13; P=0.000), and the days between the fracture and the operation (OR, 1.10; 95% CI, 1.02-1.20; P=0.020) were the independent risk factors of preoperative DVT.
29713152	8	80	theme	independent	1236:1246	arg1	thrombosis					1072:1081	venous thrombosis	1065:1081	venous thrombosis at admission (OR, 4.05; 95% CI, 2.30-7.13; P=0.000), and the days between the fracture	1065:1168	Age (odds ratio [OR], 1.03; 95% CI: 1.01-1.04; P=0.002), venous thrombosis at admission (OR, 4.05; 95% CI, 2.30-7.13; P=0.000), and the days between the fracture and the operation (OR, 1.10; 95% CI, 1.02-1.20; P=0.020) were the independent risk factors of preoperative DVT.
29713152	8	80	theme	independent	1236:1246	arg1	factors					1253:1259	the independent risk factors	1232:1259	the independent risk factors of preoperative DVT	1232:1279	Age (odds ratio [OR], 1.03; 95% CI: 1.01-1.04; P=0.002), venous thrombosis at admission (OR, 4.05; 95% CI, 2.30-7.13; P=0.000), and the days between the fracture and the operation (OR, 1.10; 95% CI, 1.02-1.20; P=0.020) were the independent risk factors of preoperative DVT.
29713152	8	80	theme	independent	1236:1246	arg1	operation					1178:1186	the operation	1174:1186	the operation (OR, 1.10; 95% CI, 1.02-1.20; P=0.020)	1174:1225	Age (odds ratio [OR], 1.03; 95% CI: 1.01-1.04; P=0.002), venous thrombosis at admission (OR, 4.05; 95% CI, 2.30-7.13; P=0.000), and the days between the fracture and the operation (OR, 1.10; 95% CI, 1.02-1.20; P=0.020) were the independent risk factors of preoperative DVT.
29713152	7	81	theme	composition	893:903	arg1	ratio					905:909	This composition ratio	888:909	This composition ratio	888:909	This composition ratio increased to 57.23% postoperatively, and the prevalence of DVT on the uninjured side was 25.05%.
29713152	2	82	theme	patients	327:334	arg1	data					315:318	the clinical data	302:318	the clinical data of 463 patients with lower extremities fractures who presented at Xi'an Honghui Hospital between July 1, 2014, and October 31, 2016 and met all the inclusion criteria	302:485	PATIENTS AND METHODS We collected the clinical data of 463 patients with lower extremities fractures who presented at Xi'an Honghui Hospital between July 1, 2014, and October 31, 2016 and met all the inclusion criteria.
30599911	13	0	dep	suggested	1844:1852	arg1	decreased					1946:1954	decreased	1946:1954	decreased the generation of gut-derived endotoxin	1946:1994	The further investigations suggested SHE dramatically reversed ethanol-induced alterations in the intestinal microbial flora and decreased the generation of gut-derived endotoxin.
30599911	13	0	dep	suggested	1844:1852	arg1	reversed					1871:1878	reversed	1871:1878	reversed ethanol-induced alterations in the intestinal microbial flora	1871:1940	The further investigations suggested SHE dramatically reversed ethanol-induced alterations in the intestinal microbial flora and decreased the generation of gut-derived endotoxin.
30599911	13	1	theme	further	1821:1827	arg1	investigations					1829:1842	The further investigations	1817:1842	The further investigations	1817:1842	The further investigations suggested SHE dramatically reversed ethanol-induced alterations in the intestinal microbial flora and decreased the generation of gut-derived endotoxin.
30599911	6	2	theme	control	883:889	arg1	diet					891:894	control diet	883:894	control diet with or without SHE	883:914	METHODS In the present study, the Sprague-Dawley rats were fed with the Lieber-DeCarli diet containing alcohol or isocaloric maltose dextrin as control diet with or without SHE (300 and 600 mg/kg/d bw) for 8 weeks.
30599911	6	3	theme	isocaloric	853:862	arg1	dextrin					872:878	isocaloric maltose dextrin	853:878	isocaloric maltose dextrin	853:878	METHODS In the present study, the Sprague-Dawley rats were fed with the Lieber-DeCarli diet containing alcohol or isocaloric maltose dextrin as control diet with or without SHE (300 and 600 mg/kg/d bw) for 8 weeks.
30599911	2	4	theme	liver	271:275	arg1	disease					277:283	ethanol-induced liver disease	255:283	ethanol-induced liver disease for centuries	255:297	is considered as a traditional herbal medicine that has been used in the treatment for ethanol-induced liver disease for centuries.
30599911	9	5	theme	western	1391:1397	arg1	blot					1399:1402	western blot	1391:1402	western blot	1391:1402	The expressions of CD14, TLR4, MyD88, NF-κB, Iκ-B, P-Iκ-B and TNF-α in liver, and ZO-1 and occludin in intestine were determined by western blot.
30599911	9	6	from	NF-κB	1297:1301	arg1	intestine					1362:1370	intestine	1362:1370	intestine	1362:1370	The expressions of CD14, TLR4, MyD88, NF-κB, Iκ-B, P-Iκ-B and TNF-α in liver, and ZO-1 and occludin in intestine were determined by western blot.
30599911	9	6	from	NF-κB	1297:1301	arg1	liver					1330:1334	liver	1330:1334	liver	1330:1334	The expressions of CD14, TLR4, MyD88, NF-κB, Iκ-B, P-Iκ-B and TNF-α in liver, and ZO-1 and occludin in intestine were determined by western blot.
30599911	3	7	theme	substantial	310:320	arg1	studies					322:328	substantial studies	310:328	substantial studies	310:328	Recently, substantial studies demonstrated that Semen hoveniae extract (SHE) not only suppressed the hepatic steatosis caused by chronic ethanol exposure, but also inhibited lipopolysaccharide-stimulated inflammatory responses.
30599911	13	8	theme	endotoxin	1986:1994	arg1	generation					1960:1969	the generation	1956:1969	the generation of gut-derived endotoxin	1956:1994	The further investigations suggested SHE dramatically reversed ethanol-induced alterations in the intestinal microbial flora and decreased the generation of gut-derived endotoxin.
30599911	11	9	from	response	1622:1629	arg1	liver					1634:1638	liver	1634:1638	liver induced by ethanol	1634:1657	RESULTS Biochemical and histopathological analysis revealed that SHE significantly alleviated the lipid deposition and inflammation response in liver induced by ethanol.
30599911	9	10	from	MyD88	1290:1294	arg1	intestine					1362:1370	intestine	1362:1370	intestine	1362:1370	The expressions of CD14, TLR4, MyD88, NF-κB, Iκ-B, P-Iκ-B and TNF-α in liver, and ZO-1 and occludin in intestine were determined by western blot.
30599911	9	10	from	MyD88	1290:1294	arg1	liver					1330:1334	liver	1330:1334	liver	1330:1334	The expressions of CD14, TLR4, MyD88, NF-κB, Iκ-B, P-Iκ-B and TNF-α in liver, and ZO-1 and occludin in intestine were determined by western blot.
30599911	0	11	theme	gut-liver	119:127	arg1	axis					129:132	gut-liver axis	119:132	gut-liver axis	119:132	Semen hoveniae extract ameliorates alcohol-induced chronic liver damage in rats via modulation of the abnormalities of gut-liver axis.
30599911	15	12	theme	therapeutic	2232:2242	arg1	options					2244:2250	a traditional therapeutic options	2218:2250	a traditional therapeutic options which may play an essential role in protecting against the chronic ethanol-triggered liver injury	2218:2348	These findings suggested that SHE as a traditional therapeutic options which may play an essential role in protecting against the chronic ethanol-triggered liver injury.
30599911	9	13	from	TLR4	1284:1287	arg1	intestine					1362:1370	intestine	1362:1370	intestine	1362:1370	The expressions of CD14, TLR4, MyD88, NF-κB, Iκ-B, P-Iκ-B and TNF-α in liver, and ZO-1 and occludin in intestine were determined by western blot.
30599911	9	13	from	TLR4	1284:1287	arg1	liver					1330:1334	liver	1330:1334	liver	1330:1334	The expressions of CD14, TLR4, MyD88, NF-κB, Iκ-B, P-Iκ-B and TNF-α in liver, and ZO-1 and occludin in intestine were determined by western blot.
30599911	15	14	theme	chronic	2311:2317	arg1	injury					2343:2348	the chronic ethanol-triggered liver injury	2307:2348	the chronic ethanol-triggered liver injury	2307:2348	These findings suggested that SHE as a traditional therapeutic options which may play an essential role in protecting against the chronic ethanol-triggered liver injury.
30599911	7	15	theme	detection	1066:1074	arg1	kit					1080:1082	endotoxin detection LAL kit	1056:1082	endotoxin detection LAL kit	1056:1082	The levels of serum biomarkers (ALT, AST and LDH) and LPS were detected by biochemical assay kits and endotoxin detection LAL kit, respectively.
30599911	9	16	from	CD14	1278:1281	arg1	intestine					1362:1370	intestine	1362:1370	intestine	1362:1370	The expressions of CD14, TLR4, MyD88, NF-κB, Iκ-B, P-Iκ-B and TNF-α in liver, and ZO-1 and occludin in intestine were determined by western blot.
30599911	9	16	from	CD14	1278:1281	arg1	liver					1330:1334	liver	1330:1334	liver	1330:1334	The expressions of CD14, TLR4, MyD88, NF-κB, Iκ-B, P-Iκ-B and TNF-α in liver, and ZO-1 and occludin in intestine were determined by western blot.
30599911	7	17	theme	LPS	1008:1010	arg1	levels					958:963	The levels	954:963	The levels of serum biomarkers (ALT, AST and LDH) and LPS	954:1010	The levels of serum biomarkers (ALT, AST and LDH) and LPS were detected by biochemical assay kits and endotoxin detection LAL kit, respectively.
30599911	5	18	from	effects	642:648	arg1	damage					684:689	ethanol-triggered liver damage	660:689	ethanol-triggered liver damage	660:689	AIM To determine the hepatoprotective effects of SHE on ethanol-triggered liver damage and further elucidate its potential mechanisms.
30599911	14	19	theme	inflammatory	2105:2116	arg1	mediators					2118:2126	TLR4-associated inflammatory mediators	2089:2126	TLR4-associated inflammatory mediators activation	2089:2137	CONCLUSION In summary, SHE probably modulated abnormalities of gut-liver axis and inhibited TLR4-associated inflammatory mediators activation to exert its hepatoprotective properties.
30599911	7	20	theme	assay	1041:1045	arg1	kits					1047:1050	biochemical assay kits	1029:1050	biochemical assay kits	1029:1050	The levels of serum biomarkers (ALT, AST and LDH) and LPS were detected by biochemical assay kits and endotoxin detection LAL kit, respectively.
30599911	14	21	dep	CONCLUSION	1997:2006	arg1	summary					2011:2017	summary	2011:2017	summary	2011:2017	CONCLUSION In summary, SHE probably modulated abnormalities of gut-liver axis and inhibited TLR4-associated inflammatory mediators activation to exert its hepatoprotective properties.
30599911	1	22	theme	BACKGROUND	135:144	arg1	Thunb					161:165	BACKGROUND Hovenia dulcis Thunb	135:165	BACKGROUND Hovenia dulcis Thunb.	135:166	BACKGROUND Hovenia dulcis Thunb.
30599911	12	23	theme	inflammatory	1721:1732	arg1	mediators					1734:1742	its downstream inflammatory mediators	1706:1742	its downstream inflammatory mediators	1706:1742	SHE remarkably inhibited the TLR4 pathway and its downstream inflammatory mediators, and up-regulated the expressions of ZO-1 and occludin in the intestine.
30599911	9	24	theme	CD14	1278:1281	arg1	expressions					1263:1273	The expressions	1259:1273	The expressions of CD14, TLR4, MyD88, NF-κB, Iκ-B, P-Iκ-B and TNF-α in liver, and ZO-1 and occludin in intestine	1259:1370	The expressions of CD14, TLR4, MyD88, NF-κB, Iκ-B, P-Iκ-B and TNF-α in liver, and ZO-1 and occludin in intestine were determined by western blot.
30599911	1	25	theme	dulcis	154:159	arg1	Thunb					161:165	BACKGROUND Hovenia dulcis Thunb	135:165	BACKGROUND Hovenia dulcis Thunb.	135:166	BACKGROUND Hovenia dulcis Thunb.
30599911	13	26	theme	intestinal	1915:1924	arg1	flora					1936:1940	the intestinal microbial flora	1911:1940	the intestinal microbial flora	1911:1940	The further investigations suggested SHE dramatically reversed ethanol-induced alterations in the intestinal microbial flora and decreased the generation of gut-derived endotoxin.
30599911	9	27	theme	TLR4	1284:1287	arg1	expressions					1263:1273	The expressions	1259:1273	The expressions of CD14, TLR4, MyD88, NF-κB, Iκ-B, P-Iκ-B and TNF-α in liver, and ZO-1 and occludin in intestine	1259:1370	The expressions of CD14, TLR4, MyD88, NF-κB, Iκ-B, P-Iκ-B and TNF-α in liver, and ZO-1 and occludin in intestine were determined by western blot.
30599911	15	28	theme	essential	2270:2278	arg1	role					2280:2283	an essential role	2267:2283	an essential role	2267:2283	These findings suggested that SHE as a traditional therapeutic options which may play an essential role in protecting against the chronic ethanol-triggered liver injury.
30599911	6	29	contain	containing	831:840	arg2	dextrin					872:878	isocaloric maltose dextrin	853:878	isocaloric maltose dextrin	853:878	METHODS In the present study, the Sprague-Dawley rats were fed with the Lieber-DeCarli diet containing alcohol or isocaloric maltose dextrin as control diet with or without SHE (300 and 600 mg/kg/d bw) for 8 weeks.
30599911	6	29	contain	containing	831:840	arg1	bw					937:938	300 and 600 mg/kg/d bw	917:938	300 and 600 mg/kg/d bw	917:938	METHODS In the present study, the Sprague-Dawley rats were fed with the Lieber-DeCarli diet containing alcohol or isocaloric maltose dextrin as control diet with or without SHE (300 and 600 mg/kg/d bw) for 8 weeks.
30599911	6	29	contain	containing	831:840	arg2	alcohol					842:848	alcohol	842:848	alcohol	842:848	METHODS In the present study, the Sprague-Dawley rats were fed with the Lieber-DeCarli diet containing alcohol or isocaloric maltose dextrin as control diet with or without SHE (300 and 600 mg/kg/d bw) for 8 weeks.
30599911	6	29	contain	containing	831:840	arg1	diet					826:829	the Lieber-DeCarli diet	807:829	the Lieber-DeCarli diet containing alcohol or isocaloric maltose dextrin as control diet with or without SHE (300 and 600 mg/kg/d bw)	807:939	METHODS In the present study, the Sprague-Dawley rats were fed with the Lieber-DeCarli diet containing alcohol or isocaloric maltose dextrin as control diet with or without SHE (300 and 600 mg/kg/d bw) for 8 weeks.
30599911	7	30	dep	biomarkers	974:983	arg1	ALT					986:988	ALT	986:988	ALT	986:988	The levels of serum biomarkers (ALT, AST and LDH) and LPS were detected by biochemical assay kits and endotoxin detection LAL kit, respectively.
30599911	7	30	dep	biomarkers	974:983	arg1	biomarkers					974:983	serum biomarkers	968:983	serum biomarkers (ALT, AST and LDH)	968:1002	The levels of serum biomarkers (ALT, AST and LDH) and LPS were detected by biochemical assay kits and endotoxin detection LAL kit, respectively.
30599911	7	30	dep	biomarkers	974:983	arg1	LDH					999:1001	LDH	999:1001	LDH	999:1001	The levels of serum biomarkers (ALT, AST and LDH) and LPS were detected by biochemical assay kits and endotoxin detection LAL kit, respectively.
30599911	7	30	dep	biomarkers	974:983	arg1	AST					991:993	AST	991:993	AST	991:993	The levels of serum biomarkers (ALT, AST and LDH) and LPS were detected by biochemical assay kits and endotoxin detection LAL kit, respectively.
30599911	9	31	theme	MyD88	1290:1294	arg1	expressions					1263:1273	The expressions	1259:1273	The expressions of CD14, TLR4, MyD88, NF-κB, Iκ-B, P-Iκ-B and TNF-α in liver, and ZO-1 and occludin in intestine	1259:1370	The expressions of CD14, TLR4, MyD88, NF-κB, Iκ-B, P-Iκ-B and TNF-α in liver, and ZO-1 and occludin in intestine were determined by western blot.
30599911	14	32	theme	axis	2070:2073	arg1	abnormalities					2043:2055	abnormalities	2043:2055	abnormalities of gut-liver axis	2043:2073	CONCLUSION In summary, SHE probably modulated abnormalities of gut-liver axis and inhibited TLR4-associated inflammatory mediators activation to exert its hepatoprotective properties.
30599911	3	33	theme	hoveniae	354:361	arg1	extract					363:369	Semen hoveniae extract	348:369	Semen hoveniae extract (SHE)	348:375	Recently, substantial studies demonstrated that Semen hoveniae extract (SHE) not only suppressed the hepatic steatosis caused by chronic ethanol exposure, but also inhibited lipopolysaccharide-stimulated inflammatory responses.
30599911	13	34	theme	ethanol-induced	1880:1894	arg1	alterations					1896:1906	ethanol-induced alterations	1880:1906	ethanol-induced alterations in the intestinal microbial flora	1880:1940	The further investigations suggested SHE dramatically reversed ethanol-induced alterations in the intestinal microbial flora and decreased the generation of gut-derived endotoxin.
30599911	3	35	theme	ethanol	437:443	arg1	exposure					445:452	chronic ethanol exposure	429:452	chronic ethanol exposure	429:452	Recently, substantial studies demonstrated that Semen hoveniae extract (SHE) not only suppressed the hepatic steatosis caused by chronic ethanol exposure, but also inhibited lipopolysaccharide-stimulated inflammatory responses.
30599911	0	36	theme	abnormalities	102:114	arg1	modulation					84:93	modulation	84:93	modulation of the abnormalities of gut-liver axis	84:132	Semen hoveniae extract ameliorates alcohol-induced chronic liver damage in rats via modulation of the abnormalities of gut-liver axis.
30599911	9	37	theme	NF-κB	1297:1301	arg1	expressions					1263:1273	The expressions	1259:1273	The expressions of CD14, TLR4, MyD88, NF-κB, Iκ-B, P-Iκ-B and TNF-α in liver, and ZO-1 and occludin in intestine	1259:1370	The expressions of CD14, TLR4, MyD88, NF-κB, Iκ-B, P-Iκ-B and TNF-α in liver, and ZO-1 and occludin in intestine were determined by western blot.
30599911	7	38	theme	serum	968:972	arg1	ALT					986:988	ALT	986:988	ALT	986:988	The levels of serum biomarkers (ALT, AST and LDH) and LPS were detected by biochemical assay kits and endotoxin detection LAL kit, respectively.
30599911	7	38	theme	serum	968:972	arg1	biomarkers					974:983	serum biomarkers	968:983	serum biomarkers (ALT, AST and LDH)	968:1002	The levels of serum biomarkers (ALT, AST and LDH) and LPS were detected by biochemical assay kits and endotoxin detection LAL kit, respectively.
30599911	7	38	theme	serum	968:972	arg1	LDH					999:1001	LDH	999:1001	LDH	999:1001	The levels of serum biomarkers (ALT, AST and LDH) and LPS were detected by biochemical assay kits and endotoxin detection LAL kit, respectively.
30599911	7	38	theme	serum	968:972	arg1	AST					991:993	AST	991:993	AST	991:993	The levels of serum biomarkers (ALT, AST and LDH) and LPS were detected by biochemical assay kits and endotoxin detection LAL kit, respectively.
30599911	10	39	theme	faecal	1409:1414	arg1	composition					1426:1436	The faecal microbial composition	1405:1436	The faecal microbial composition	1405:1436	The faecal microbial composition was determined by16S rRNA Gene Sequencing Analysis.
30599911	6	40	theme	present	754:760	arg1	study					762:766	the present study	750:766	the present study	750:766	METHODS In the present study, the Sprague-Dawley rats were fed with the Lieber-DeCarli diet containing alcohol or isocaloric maltose dextrin as control diet with or without SHE (300 and 600 mg/kg/d bw) for 8 weeks.
30599911	0	41	theme	Semen	0:4	arg1	extract					15:21	Semen hoveniae extract	0:21	Semen hoveniae extract	0:21	Semen hoveniae extract ameliorates alcohol-induced chronic liver damage in rats via modulation of the abnormalities of gut-liver axis.
30599911	9	42	theme	Iκ-B	1304:1307	arg1	expressions					1263:1273	The expressions	1259:1273	The expressions of CD14, TLR4, MyD88, NF-κB, Iκ-B, P-Iκ-B and TNF-α in liver, and ZO-1 and occludin in intestine	1259:1370	The expressions of CD14, TLR4, MyD88, NF-κB, Iκ-B, P-Iκ-B and TNF-α in liver, and ZO-1 and occludin in intestine were determined by western blot.
30599911	5	43	theme	hepatoprotective	625:640	arg1	effects					642:648	the hepatoprotective effects	621:648	the hepatoprotective effects of SHE on ethanol-triggered liver damage	621:689	AIM To determine the hepatoprotective effects of SHE on ethanol-triggered liver damage and further elucidate its potential mechanisms.
30599911	10	44	theme	rRNA	1459:1462	arg1	Analysis					1480:1487	rRNA Gene Sequencing Analysis	1459:1487	rRNA Gene Sequencing Analysis	1459:1487	The faecal microbial composition was determined by16S rRNA Gene Sequencing Analysis.
30599911	11	45	theme	Biochemical	1498:1508	arg1	analysis					1532:1539	Biochemical and histopathological analysis	1498:1539	Biochemical and histopathological analysis	1498:1539	RESULTS Biochemical and histopathological analysis revealed that SHE significantly alleviated the lipid deposition and inflammation response in liver induced by ethanol.
30599911	9	46	theme	P-Iκ-B	1310:1315	arg1	expressions					1263:1273	The expressions	1259:1273	The expressions of CD14, TLR4, MyD88, NF-κB, Iκ-B, P-Iκ-B and TNF-α in liver, and ZO-1 and occludin in intestine	1259:1370	The expressions of CD14, TLR4, MyD88, NF-κB, Iκ-B, P-Iκ-B and TNF-α in liver, and ZO-1 and occludin in intestine were determined by western blot.
30599911	0	47	theme	alcohol-induced	35:49	arg1	damage					65:70	alcohol-induced chronic liver damage	35:70	alcohol-induced chronic liver damage in rats	35:78	Semen hoveniae extract ameliorates alcohol-induced chronic liver damage in rats via modulation of the abnormalities of gut-liver axis.
30599911	9	48	from	intestine	1362:1370	arg1	expressions					1263:1273	The expressions	1259:1273	The expressions of CD14, TLR4, MyD88, NF-κB, Iκ-B, P-Iκ-B and TNF-α in liver, and ZO-1 and occludin in intestine	1259:1370	The expressions of CD14, TLR4, MyD88, NF-κB, Iκ-B, P-Iκ-B and TNF-α in liver, and ZO-1 and occludin in intestine were determined by western blot.
30599911	4	49	theme	molecular	557:565	arg1	mechanisms					567:576	the underlying molecular mechanisms	542:576	the underlying molecular mechanisms	542:576	Nevertheless, the underlying molecular mechanisms largely remained elusive.
30599911	14	50	theme	mediators	2118:2126	arg1	activation					2128:2137	TLR4-associated inflammatory mediators activation	2089:2137	TLR4-associated inflammatory mediators activation	2089:2137	CONCLUSION In summary, SHE probably modulated abnormalities of gut-liver axis and inhibited TLR4-associated inflammatory mediators activation to exert its hepatoprotective properties.
30599911	11	51	theme	lipid	1588:1592	arg1	deposition					1594:1603	lipid deposition	1588:1603	lipid deposition	1588:1603	RESULTS Biochemical and histopathological analysis revealed that SHE significantly alleviated the lipid deposition and inflammation response in liver induced by ethanol.
30599911	0	52	theme	liver	59:63	arg1	damage					65:70	alcohol-induced chronic liver damage	35:70	alcohol-induced chronic liver damage in rats	35:78	Semen hoveniae extract ameliorates alcohol-induced chronic liver damage in rats via modulation of the abnormalities of gut-liver axis.
30599911	15	53	theme	ethanol-triggered	2319:2335	arg1	injury					2343:2348	the chronic ethanol-triggered liver injury	2307:2348	the chronic ethanol-triggered liver injury	2307:2348	These findings suggested that SHE as a traditional therapeutic options which may play an essential role in protecting against the chronic ethanol-triggered liver injury.
30599911	8	54	theme	histopathological	1103:1119	arg1	changes					1121:1127	The histopathological changes	1099:1127	The histopathological changes of liver and intestinal tissues	1099:1159	The histopathological changes of liver and intestinal tissues were observed by hematoxylin and eosin (H&E) staining and Transmission electron microscope (TEM).
30599911	11	55	dep	RESULTS	1490:1496	arg1	revealed					1541:1548	revealed	1541:1548	revealed that SHE significantly alleviated the lipid deposition and inflammation response in liver induced by ethanol	1541:1657	RESULTS Biochemical and histopathological analysis revealed that SHE significantly alleviated the lipid deposition and inflammation response in liver induced by ethanol.
30599911	10	56	theme	Sequencing	1469:1478	arg1	Analysis					1480:1487	rRNA Gene Sequencing Analysis	1459:1487	rRNA Gene Sequencing Analysis	1459:1487	The faecal microbial composition was determined by16S rRNA Gene Sequencing Analysis.
30599911	3	57	theme	lipopolysaccharide-stimulated	474:502	arg1	responses					517:525	lipopolysaccharide-stimulated inflammatory responses	474:525	lipopolysaccharide-stimulated inflammatory responses	474:525	Recently, substantial studies demonstrated that Semen hoveniae extract (SHE) not only suppressed the hepatic steatosis caused by chronic ethanol exposure, but also inhibited lipopolysaccharide-stimulated inflammatory responses.
30599911	9	58	from	ZO-1	1341:1344	arg1	intestine					1362:1370	intestine	1362:1370	intestine	1362:1370	The expressions of CD14, TLR4, MyD88, NF-κB, Iκ-B, P-Iκ-B and TNF-α in liver, and ZO-1 and occludin in intestine were determined by western blot.
30599911	9	58	from	ZO-1	1341:1344	arg1	liver					1330:1334	liver	1330:1334	liver	1330:1334	The expressions of CD14, TLR4, MyD88, NF-κB, Iκ-B, P-Iκ-B and TNF-α in liver, and ZO-1 and occludin in intestine were determined by western blot.
30599911	12	59	theme	occludin	1790:1797	arg1	expressions					1766:1776	the expressions	1762:1776	the expressions of ZO-1 and occludin in the intestine	1762:1814	SHE remarkably inhibited the TLR4 pathway and its downstream inflammatory mediators, and up-regulated the expressions of ZO-1 and occludin in the intestine.
30599911	2	60	theme	traditional	187:197	arg1	medicine					206:213	a traditional herbal medicine	185:213	a traditional herbal medicine that has been used in the treatment for ethanol-induced liver disease for centuries	185:297	is considered as a traditional herbal medicine that has been used in the treatment for ethanol-induced liver disease for centuries.
30599911	6	61	theme	Sprague-Dawley	773:786	arg1	rats					788:791	the Sprague-Dawley rats	769:791	the Sprague-Dawley rats	769:791	METHODS In the present study, the Sprague-Dawley rats were fed with the Lieber-DeCarli diet containing alcohol or isocaloric maltose dextrin as control diet with or without SHE (300 and 600 mg/kg/d bw) for 8 weeks.
30599911	8	62	theme	tissues	1153:1159	arg1	changes					1121:1127	The histopathological changes	1099:1127	The histopathological changes of liver and intestinal tissues	1099:1159	The histopathological changes of liver and intestinal tissues were observed by hematoxylin and eosin (H&E) staining and Transmission electron microscope (TEM).
30599911	5	63	theme	potential	717:725	arg1	mechanisms					727:736	its potential mechanisms	713:736	its potential mechanisms	713:736	AIM To determine the hepatoprotective effects of SHE on ethanol-triggered liver damage and further elucidate its potential mechanisms.
30599911	11	64	theme	histopathological	1514:1530	arg1	analysis					1532:1539	Biochemical and histopathological analysis	1498:1539	Biochemical and histopathological analysis	1498:1539	RESULTS Biochemical and histopathological analysis revealed that SHE significantly alleviated the lipid deposition and inflammation response in liver induced by ethanol.
30599911	6	65	theme	Lieber-DeCarli	811:824	arg1	bw					937:938	300 and 600 mg/kg/d bw	917:938	300 and 600 mg/kg/d bw	917:938	METHODS In the present study, the Sprague-Dawley rats were fed with the Lieber-DeCarli diet containing alcohol or isocaloric maltose dextrin as control diet with or without SHE (300 and 600 mg/kg/d bw) for 8 weeks.
30599911	6	65	theme	Lieber-DeCarli	811:824	arg1	diet					826:829	the Lieber-DeCarli diet	807:829	the Lieber-DeCarli diet containing alcohol or isocaloric maltose dextrin as control diet with or without SHE (300 and 600 mg/kg/d bw)	807:939	METHODS In the present study, the Sprague-Dawley rats were fed with the Lieber-DeCarli diet containing alcohol or isocaloric maltose dextrin as control diet with or without SHE (300 and 600 mg/kg/d bw) for 8 weeks.
30599911	12	66	theme	ZO-1	1781:1784	arg1	expressions					1766:1776	the expressions	1762:1776	the expressions of ZO-1 and occludin in the intestine	1762:1814	SHE remarkably inhibited the TLR4 pathway and its downstream inflammatory mediators, and up-regulated the expressions of ZO-1 and occludin in the intestine.
30599911	5	67	theme	liver	678:682	arg1	damage					684:689	ethanol-triggered liver damage	660:689	ethanol-triggered liver damage	660:689	AIM To determine the hepatoprotective effects of SHE on ethanol-triggered liver damage and further elucidate its potential mechanisms.
30599911	6	68	theme	maltose	864:870	arg1	dextrin					872:878	isocaloric maltose dextrin	853:878	isocaloric maltose dextrin	853:878	METHODS In the present study, the Sprague-Dawley rats were fed with the Lieber-DeCarli diet containing alcohol or isocaloric maltose dextrin as control diet with or without SHE (300 and 600 mg/kg/d bw) for 8 weeks.
30599911	11	69	from	deposition	1594:1603	arg1	liver					1634:1638	liver	1634:1638	liver induced by ethanol	1634:1657	RESULTS Biochemical and histopathological analysis revealed that SHE significantly alleviated the lipid deposition and inflammation response in liver induced by ethanol.
30599911	8	70	theme	hematoxylin	1178:1188	arg1	staining					1206:1213	hematoxylin and eosin (H&E) staining	1178:1213	hematoxylin and eosin (H&E) staining	1178:1213	The histopathological changes of liver and intestinal tissues were observed by hematoxylin and eosin (H&E) staining and Transmission electron microscope (TEM).
30599911	13	71	theme	gut-derived	1974:1984	arg1	endotoxin					1986:1994	gut-derived endotoxin	1974:1994	gut-derived endotoxin	1974:1994	The further investigations suggested SHE dramatically reversed ethanol-induced alterations in the intestinal microbial flora and decreased the generation of gut-derived endotoxin.
30599911	9	72	from	occludin	1350:1357	arg1	intestine					1362:1370	intestine	1362:1370	intestine	1362:1370	The expressions of CD14, TLR4, MyD88, NF-κB, Iκ-B, P-Iκ-B and TNF-α in liver, and ZO-1 and occludin in intestine were determined by western blot.
30599911	9	72	from	occludin	1350:1357	arg1	liver					1330:1334	liver	1330:1334	liver	1330:1334	The expressions of CD14, TLR4, MyD88, NF-κB, Iκ-B, P-Iκ-B and TNF-α in liver, and ZO-1 and occludin in intestine were determined by western blot.
30599911	8	73	theme	eosin	1194:1198	arg1	staining					1206:1213	hematoxylin and eosin (H&E) staining	1178:1213	hematoxylin and eosin (H&E) staining	1178:1213	The histopathological changes of liver and intestinal tissues were observed by hematoxylin and eosin (H&E) staining and Transmission electron microscope (TEM).
30599911	11	74	dep	deposition	1594:1603	arg1	the					1584:1586	the	1584:1586	the	1584:1586	RESULTS Biochemical and histopathological analysis revealed that SHE significantly alleviated the lipid deposition and inflammation response in liver induced by ethanol.
30599911	7	75	theme	LAL	1076:1078	arg1	kit					1080:1082	endotoxin detection LAL kit	1056:1082	endotoxin detection LAL kit	1056:1082	The levels of serum biomarkers (ALT, AST and LDH) and LPS were detected by biochemical assay kits and endotoxin detection LAL kit, respectively.
30599911	15	76	theme	traditional	2220:2230	arg1	options					2244:2250	a traditional therapeutic options	2218:2250	a traditional therapeutic options which may play an essential role in protecting against the chronic ethanol-triggered liver injury	2218:2348	These findings suggested that SHE as a traditional therapeutic options which may play an essential role in protecting against the chronic ethanol-triggered liver injury.
30599911	2	77	used	used	229:232	arg2	medicine					206:213	a traditional herbal medicine	185:213	a traditional herbal medicine that has been used in the treatment for ethanol-induced liver disease for centuries	185:297	is considered as a traditional herbal medicine that has been used in the treatment for ethanol-induced liver disease for centuries.
30599911	7	78	theme	endotoxin	1056:1064	arg1	kit					1080:1082	endotoxin detection LAL kit	1056:1082	endotoxin detection LAL kit	1056:1082	The levels of serum biomarkers (ALT, AST and LDH) and LPS were detected by biochemical assay kits and endotoxin detection LAL kit, respectively.
30599911	14	79	theme	TLR4-associated	2089:2103	arg1	mediators					2118:2126	TLR4-associated inflammatory mediators	2089:2126	TLR4-associated inflammatory mediators activation	2089:2137	CONCLUSION In summary, SHE probably modulated abnormalities of gut-liver axis and inhibited TLR4-associated inflammatory mediators activation to exert its hepatoprotective properties.
30599911	8	80	theme	Transmission	1219:1230	arg1	TEM					1253:1255	TEM	1253:1255	TEM	1253:1255	The histopathological changes of liver and intestinal tissues were observed by hematoxylin and eosin (H&E) staining and Transmission electron microscope (TEM).
30599911	8	80	theme	Transmission	1219:1230	arg1	microscope					1241:1250	Transmission electron microscope	1219:1250	Transmission electron microscope (TEM)	1219:1256	The histopathological changes of liver and intestinal tissues were observed by hematoxylin and eosin (H&E) staining and Transmission electron microscope (TEM).
30599911	0	81	theme	axis	129:132	arg1	abnormalities					102:114	the abnormalities	98:114	the abnormalities of gut-liver axis	98:132	Semen hoveniae extract ameliorates alcohol-induced chronic liver damage in rats via modulation of the abnormalities of gut-liver axis.
30599911	7	82	theme	biochemical	1029:1039	arg1	kits					1047:1050	biochemical assay kits	1029:1050	biochemical assay kits	1029:1050	The levels of serum biomarkers (ALT, AST and LDH) and LPS were detected by biochemical assay kits and endotoxin detection LAL kit, respectively.
30599911	2	83	theme	ethanol-induced	255:269	arg1	disease					277:283	ethanol-induced liver disease	255:283	ethanol-induced liver disease for centuries	255:297	is considered as a traditional herbal medicine that has been used in the treatment for ethanol-induced liver disease for centuries.
30599911	1	84	theme	Hovenia	146:152	arg1	Thunb					161:165	BACKGROUND Hovenia dulcis Thunb	135:165	BACKGROUND Hovenia dulcis Thunb.	135:166	BACKGROUND Hovenia dulcis Thunb.
30599911	12	85	theme	downstream	1710:1719	arg1	mediators					1734:1742	its downstream inflammatory mediators	1706:1742	its downstream inflammatory mediators	1706:1742	SHE remarkably inhibited the TLR4 pathway and its downstream inflammatory mediators, and up-regulated the expressions of ZO-1 and occludin in the intestine.
30599911	3	86	theme	Semen	348:352	arg1	extract					363:369	Semen hoveniae extract	348:369	Semen hoveniae extract (SHE)	348:375	Recently, substantial studies demonstrated that Semen hoveniae extract (SHE) not only suppressed the hepatic steatosis caused by chronic ethanol exposure, but also inhibited lipopolysaccharide-stimulated inflammatory responses.
30599911	8	87	theme	intestinal	1142:1151	arg1	tissues					1153:1159	intestinal tissues	1142:1159	intestinal tissues	1142:1159	The histopathological changes of liver and intestinal tissues were observed by hematoxylin and eosin (H&E) staining and Transmission electron microscope (TEM).
30599911	9	88	from	liver	1330:1334	arg1	expressions					1263:1273	The expressions	1259:1273	The expressions of CD14, TLR4, MyD88, NF-κB, Iκ-B, P-Iκ-B and TNF-α in liver, and ZO-1 and occludin in intestine	1259:1370	The expressions of CD14, TLR4, MyD88, NF-κB, Iκ-B, P-Iκ-B and TNF-α in liver, and ZO-1 and occludin in intestine were determined by western blot.
30599911	4	89	theme	underlying	546:555	arg1	mechanisms					567:576	the underlying molecular mechanisms	542:576	the underlying molecular mechanisms	542:576	Nevertheless, the underlying molecular mechanisms largely remained elusive.
30599911	6	90	dep	METHODS	739:745	arg1	fed					798:800	fed	798:800	were fed with the Lieber-DeCarli diet containing alcohol or isocaloric maltose dextrin as control diet with or without SHE (300 and 600 mg/kg/d bw) for 8 weeks	793:951	METHODS In the present study, the Sprague-Dawley rats were fed with the Lieber-DeCarli diet containing alcohol or isocaloric maltose dextrin as control diet with or without SHE (300 and 600 mg/kg/d bw) for 8 weeks.
30599911	13	91	theme	microbial	1926:1934	arg1	flora					1936:1940	the intestinal microbial flora	1911:1940	the intestinal microbial flora	1911:1940	The further investigations suggested SHE dramatically reversed ethanol-induced alterations in the intestinal microbial flora and decreased the generation of gut-derived endotoxin.
30599911	9	92	from	TNF-α	1321:1325	arg1	intestine					1362:1370	intestine	1362:1370	intestine	1362:1370	The expressions of CD14, TLR4, MyD88, NF-κB, Iκ-B, P-Iκ-B and TNF-α in liver, and ZO-1 and occludin in intestine were determined by western blot.
30599911	9	92	from	TNF-α	1321:1325	arg1	liver					1330:1334	liver	1330:1334	liver	1330:1334	The expressions of CD14, TLR4, MyD88, NF-κB, Iκ-B, P-Iκ-B and TNF-α in liver, and ZO-1 and occludin in intestine were determined by western blot.
30599911	5	93	theme	SHE	653:655	arg1	effects					642:648	the hepatoprotective effects	621:648	the hepatoprotective effects of SHE on ethanol-triggered liver damage	621:689	AIM To determine the hepatoprotective effects of SHE on ethanol-triggered liver damage and further elucidate its potential mechanisms.
30599911	12	94	theme	TLR4	1689:1692	arg1	pathway					1694:1700	the TLR4 pathway	1685:1700	the TLR4 pathway	1685:1700	SHE remarkably inhibited the TLR4 pathway and its downstream inflammatory mediators, and up-regulated the expressions of ZO-1 and occludin in the intestine.
30599911	0	95	from	damage	65:70	arg1	rats					75:78	rats	75:78	rats	75:78	Semen hoveniae extract ameliorates alcohol-induced chronic liver damage in rats via modulation of the abnormalities of gut-liver axis.
30599911	9	96	from	P-Iκ-B	1310:1315	arg1	intestine					1362:1370	intestine	1362:1370	intestine	1362:1370	The expressions of CD14, TLR4, MyD88, NF-κB, Iκ-B, P-Iκ-B and TNF-α in liver, and ZO-1 and occludin in intestine were determined by western blot.
30599911	9	96	from	P-Iκ-B	1310:1315	arg1	liver					1330:1334	liver	1330:1334	liver	1330:1334	The expressions of CD14, TLR4, MyD88, NF-κB, Iκ-B, P-Iκ-B and TNF-α in liver, and ZO-1 and occludin in intestine were determined by western blot.
30599911	5	97	theme	ethanol-triggered	660:676	arg1	damage					684:689	ethanol-triggered liver damage	660:689	ethanol-triggered liver damage	660:689	AIM To determine the hepatoprotective effects of SHE on ethanol-triggered liver damage and further elucidate its potential mechanisms.
30599911	14	98	theme	gut-liver	2060:2068	arg1	axis					2070:2073	gut-liver axis	2060:2073	gut-liver axis	2060:2073	CONCLUSION In summary, SHE probably modulated abnormalities of gut-liver axis and inhibited TLR4-associated inflammatory mediators activation to exert its hepatoprotective properties.
30599911	10	99	theme	microbial	1416:1424	arg1	composition					1426:1436	The faecal microbial composition	1405:1436	The faecal microbial composition	1405:1436	The faecal microbial composition was determined by16S rRNA Gene Sequencing Analysis.
30599911	0	100	theme	hoveniae	6:13	arg1	extract					15:21	Semen hoveniae extract	0:21	Semen hoveniae extract	0:21	Semen hoveniae extract ameliorates alcohol-induced chronic liver damage in rats via modulation of the abnormalities of gut-liver axis.
30599911	9	101	from	Iκ-B	1304:1307	arg1	intestine					1362:1370	intestine	1362:1370	intestine	1362:1370	The expressions of CD14, TLR4, MyD88, NF-κB, Iκ-B, P-Iκ-B and TNF-α in liver, and ZO-1 and occludin in intestine were determined by western blot.
30599911	9	101	from	Iκ-B	1304:1307	arg1	liver					1330:1334	liver	1330:1334	liver	1330:1334	The expressions of CD14, TLR4, MyD88, NF-κB, Iκ-B, P-Iκ-B and TNF-α in liver, and ZO-1 and occludin in intestine were determined by western blot.
30599911	7	102	theme	biomarkers	974:983	arg1	levels					958:963	The levels	954:963	The levels of serum biomarkers (ALT, AST and LDH) and LPS	954:1010	The levels of serum biomarkers (ALT, AST and LDH) and LPS were detected by biochemical assay kits and endotoxin detection LAL kit, respectively.
30599911	12	103	from	expressions	1766:1776	arg1	intestine					1806:1814	the intestine	1802:1814	the intestine	1802:1814	SHE remarkably inhibited the TLR4 pathway and its downstream inflammatory mediators, and up-regulated the expressions of ZO-1 and occludin in the intestine.
30599911	14	104	theme	hepatoprotective	2152:2167	arg1	properties					2169:2178	its hepatoprotective properties	2148:2178	its hepatoprotective properties	2148:2178	CONCLUSION In summary, SHE probably modulated abnormalities of gut-liver axis and inhibited TLR4-associated inflammatory mediators activation to exert its hepatoprotective properties.
30599911	3	105	theme	chronic	429:435	arg1	exposure					445:452	chronic ethanol exposure	429:452	chronic ethanol exposure	429:452	Recently, substantial studies demonstrated that Semen hoveniae extract (SHE) not only suppressed the hepatic steatosis caused by chronic ethanol exposure, but also inhibited lipopolysaccharide-stimulated inflammatory responses.
30599911	0	106	theme	chronic	51:57	arg1	damage					65:70	alcohol-induced chronic liver damage	35:70	alcohol-induced chronic liver damage in rats	35:78	Semen hoveniae extract ameliorates alcohol-induced chronic liver damage in rats via modulation of the abnormalities of gut-liver axis.
30599911	3	107	theme	hepatic	401:407	arg1	steatosis					409:417	the hepatic steatosis	397:417	the hepatic steatosis caused by chronic ethanol exposure	397:452	Recently, substantial studies demonstrated that Semen hoveniae extract (SHE) not only suppressed the hepatic steatosis caused by chronic ethanol exposure, but also inhibited lipopolysaccharide-stimulated inflammatory responses.
30599911	3	108	dep	extract	363:369	arg1	only					381:384	only	381:384	only	381:384	Recently, substantial studies demonstrated that Semen hoveniae extract (SHE) not only suppressed the hepatic steatosis caused by chronic ethanol exposure, but also inhibited lipopolysaccharide-stimulated inflammatory responses.
30599911	3	108	dep	extract	363:369	arg1	SHE					372:374	SHE	372:374	SHE	372:374	Recently, substantial studies demonstrated that Semen hoveniae extract (SHE) not only suppressed the hepatic steatosis caused by chronic ethanol exposure, but also inhibited lipopolysaccharide-stimulated inflammatory responses.
30599911	15	109	theme	liver	2337:2341	arg1	injury					2343:2348	the chronic ethanol-triggered liver injury	2307:2348	the chronic ethanol-triggered liver injury	2307:2348	These findings suggested that SHE as a traditional therapeutic options which may play an essential role in protecting against the chronic ethanol-triggered liver injury.
30599911	8	110	theme	liver	1132:1136	arg1	changes					1121:1127	The histopathological changes	1099:1127	The histopathological changes of liver and intestinal tissues	1099:1159	The histopathological changes of liver and intestinal tissues were observed by hematoxylin and eosin (H&E) staining and Transmission electron microscope (TEM).
30599911	13	111	from	alterations	1896:1906	arg1	flora					1936:1940	the intestinal microbial flora	1911:1940	the intestinal microbial flora	1911:1940	The further investigations suggested SHE dramatically reversed ethanol-induced alterations in the intestinal microbial flora and decreased the generation of gut-derived endotoxin.
30599911	10	112	theme	Gene	1464:1467	arg1	Analysis					1480:1487	rRNA Gene Sequencing Analysis	1459:1487	rRNA Gene Sequencing Analysis	1459:1487	The faecal microbial composition was determined by16S rRNA Gene Sequencing Analysis.
30599911	2	113	theme	herbal	199:204	arg1	medicine					206:213	a traditional herbal medicine	185:213	a traditional herbal medicine that has been used in the treatment for ethanol-induced liver disease for centuries	185:297	is considered as a traditional herbal medicine that has been used in the treatment for ethanol-induced liver disease for centuries.
30599911	11	114	theme	inflammation	1609:1620	arg1	response					1622:1629	inflammation response	1609:1629	inflammation response	1609:1629	RESULTS Biochemical and histopathological analysis revealed that SHE significantly alleviated the lipid deposition and inflammation response in liver induced by ethanol.
30599911	3	115	theme	inflammatory	504:515	arg1	responses					517:525	lipopolysaccharide-stimulated inflammatory responses	474:525	lipopolysaccharide-stimulated inflammatory responses	474:525	Recently, substantial studies demonstrated that Semen hoveniae extract (SHE) not only suppressed the hepatic steatosis caused by chronic ethanol exposure, but also inhibited lipopolysaccharide-stimulated inflammatory responses.
30599911	9	116	theme	ZO-1	1341:1344	arg1	expressions					1263:1273	The expressions	1259:1273	The expressions of CD14, TLR4, MyD88, NF-κB, Iκ-B, P-Iκ-B and TNF-α in liver, and ZO-1 and occludin in intestine	1259:1370	The expressions of CD14, TLR4, MyD88, NF-κB, Iκ-B, P-Iκ-B and TNF-α in liver, and ZO-1 and occludin in intestine were determined by western blot.
30599911	9	117	from	expressions	1263:1273	arg1	intestine					1362:1370	intestine	1362:1370	intestine	1362:1370	The expressions of CD14, TLR4, MyD88, NF-κB, Iκ-B, P-Iκ-B and TNF-α in liver, and ZO-1 and occludin in intestine were determined by western blot.
30599911	9	117	from	expressions	1263:1273	arg1	liver					1330:1334	liver	1330:1334	liver	1330:1334	The expressions of CD14, TLR4, MyD88, NF-κB, Iκ-B, P-Iκ-B and TNF-α in liver, and ZO-1 and occludin in intestine were determined by western blot.
30599911	8	118	theme	electron	1232:1239	arg1	TEM					1253:1255	TEM	1253:1255	TEM	1253:1255	The histopathological changes of liver and intestinal tissues were observed by hematoxylin and eosin (H&E) staining and Transmission electron microscope (TEM).
30599911	8	118	theme	electron	1232:1239	arg1	microscope					1241:1250	Transmission electron microscope	1219:1250	Transmission electron microscope (TEM)	1219:1256	The histopathological changes of liver and intestinal tissues were observed by hematoxylin and eosin (H&E) staining and Transmission electron microscope (TEM).
30599911	13	119	link	gut-derived	1974:1984	arg1	endotoxin					1986:1994	gut-derived endotoxin	1974:1994	gut-derived endotoxin	1974:1994	The further investigations suggested SHE dramatically reversed ethanol-induced alterations in the intestinal microbial flora and decreased the generation of gut-derived endotoxin.
29702573	5	0	from	gastropods	900:909	arg1	adenocarcinoma					928:941	human gastric adenocarcinoma	914:941	human gastric adenocarcinoma (AGS)	914:947	The results from cytotoxicity assays, using the acetonic extracts of the gastropods on human gastric adenocarcinoma (AGS) and human lung adenocarcinoma (A549) cell lines, revealed a pronounced cytotoxic effect of the A. tigrina extract on both cell lines (IC50 values of 68.75 and 69.77 μg mL−1 for AGS and A549, respectively).
29702573	5	0	from	gastropods	900:909	arg1	lines					991:995	human lung adenocarcinoma (A549) cell lines	953:995	human lung adenocarcinoma (A549) cell lines	953:995	The results from cytotoxicity assays, using the acetonic extracts of the gastropods on human gastric adenocarcinoma (AGS) and human lung adenocarcinoma (A549) cell lines, revealed a pronounced cytotoxic effect of the A. tigrina extract on both cell lines (IC50 values of 68.75 and 69.77 μg mL−1 for AGS and A549, respectively).
29702573	5	0	from	gastropods	900:909	arg1	AGS					944:946	AGS	944:946	AGS	944:946	The results from cytotoxicity assays, using the acetonic extracts of the gastropods on human gastric adenocarcinoma (AGS) and human lung adenocarcinoma (A549) cell lines, revealed a pronounced cytotoxic effect of the A. tigrina extract on both cell lines (IC50 values of 68.75 and 69.77 μg mL−1 for AGS and A549, respectively).
29702573	6	1	theme	μg	1320:1321	arg1	mL−1					1323:1326	125 μg mL−1	1316:1326	125 μg mL−1	1316:1326	It is worth noting the significant reduction of NO levels in LPS-challenged RAW 264.7 macrophages exposed to A. tricolorata extract, at concentrations as low as 125 μg mL−1.
29702573	5	2	dep	A.	1044:1045	arg1	tigrina					1047:1053	tigrina	1047:1053	tigrina	1047:1053	The results from cytotoxicity assays, using the acetonic extracts of the gastropods on human gastric adenocarcinoma (AGS) and human lung adenocarcinoma (A549) cell lines, revealed a pronounced cytotoxic effect of the A. tigrina extract on both cell lines (IC50 values of 68.75 and 69.77 μg mL−1 for AGS and A549, respectively).
29702573	6	3	theme	125	1316:1318	arg1	μg					1320:1321	μg	1320:1321	μg	1320:1321	It is worth noting the significant reduction of NO levels in LPS-challenged RAW 264.7 macrophages exposed to A. tricolorata extract, at concentrations as low as 125 μg mL−1.
29702573	6	4	theme	RAW	1231:1233	arg1	macrophages					1241:1251	LPS-challenged RAW 264.7 macrophages	1216:1251	LPS-challenged RAW 264.7 macrophages exposed to A. tricolorata extract	1216:1285	It is worth noting the significant reduction of NO levels in LPS-challenged RAW 264.7 macrophages exposed to A. tricolorata extract, at concentrations as low as 125 μg mL−1.
29702573	5	5	theme	cell	1071:1074	arg1	lines					1076:1080	both cell lines	1066:1080	both cell lines (IC50 values of 68.75 and 69.77 μg mL−1 for AGS and A549, respectively)	1066:1152	The results from cytotoxicity assays, using the acetonic extracts of the gastropods on human gastric adenocarcinoma (AGS) and human lung adenocarcinoma (A549) cell lines, revealed a pronounced cytotoxic effect of the A. tigrina extract on both cell lines (IC50 values of 68.75 and 69.77 μg mL−1 for AGS and A549, respectively).
29702573	1	6	theme	temperate	281:289	arg1	ecosystems					291:300	temperate ecosystems	281:300	temperate ecosystems	281:300	Bioprospection of marine invertebrates has been predominantly biased by the biological richness of tropical regions, thus neglecting macro-organisms from temperate ecosystems.
29702573	0	7	from	Profiling	0:8	arg1	Waters					62:67	Portuguese Coastal Waters	43:67	Portuguese Coastal Waters as Producers of Anti-Cancer and Anti-Inflammatory Agents	43:124	Profiling of Heterobranchia Sea Slugs from Portuguese Coastal Waters as Producers of Anti-Cancer and Anti-Inflammatory Agents.
29702573	6	8	theme	LPS-challenged	1216:1229	arg1	macrophages					1241:1251	LPS-challenged RAW 264.7 macrophages	1216:1251	LPS-challenged RAW 264.7 macrophages exposed to A. tricolorata extract	1216:1285	It is worth noting the significant reduction of NO levels in LPS-challenged RAW 264.7 macrophages exposed to A. tricolorata extract, at concentrations as low as 125 μg mL−1.
29702573	3	9	theme	fatty	569:573	arg1	profile					580:586	the fatty acid profile	565:586	the fatty acid profile of neutral lipids	565:604	Here, we present for the first time the fatty acid profile of neutral lipids and homarine content of these three species.
29702573	5	10	theme	lung	959:962	arg1	lines					991:995	human lung adenocarcinoma (A549) cell lines	953:995	human lung adenocarcinoma (A549) cell lines	953:995	The results from cytotoxicity assays, using the acetonic extracts of the gastropods on human gastric adenocarcinoma (AGS) and human lung adenocarcinoma (A549) cell lines, revealed a pronounced cytotoxic effect of the A. tigrina extract on both cell lines (IC50 values of 68.75 and 69.77 μg mL−1 for AGS and A549, respectively).
29702573	6	11	from	levels	1206:1211	arg1	macrophages					1241:1251	LPS-challenged RAW 264.7 macrophages	1216:1251	LPS-challenged RAW 264.7 macrophages exposed to A. tricolorata extract	1216:1285	It is worth noting the significant reduction of NO levels in LPS-challenged RAW 264.7 macrophages exposed to A. tricolorata extract, at concentrations as low as 125 μg mL−1.
29702573	0	12	theme	Anti-Cancer	85:95	arg1	Agents					119:124	Anti-Cancer and Anti-Inflammatory Agents	85:124	Anti-Cancer and Anti-Inflammatory Agents	85:124	Profiling of Heterobranchia Sea Slugs from Portuguese Coastal Waters as Producers of Anti-Cancer and Anti-Inflammatory Agents.
29702573	3	13	theme	acid	575:578	arg1	profile					580:586	the fatty acid profile	565:586	the fatty acid profile of neutral lipids	565:604	Here, we present for the first time the fatty acid profile of neutral lipids and homarine content of these three species.
29702573	5	14	theme	gastropods	900:909	arg1	extracts					884:891	the acetonic extracts	871:891	the acetonic extracts of the gastropods on human gastric adenocarcinoma (AGS) and human lung adenocarcinoma (A549) cell lines	871:995	The results from cytotoxicity assays, using the acetonic extracts of the gastropods on human gastric adenocarcinoma (AGS) and human lung adenocarcinoma (A549) cell lines, revealed a pronounced cytotoxic effect of the A. tigrina extract on both cell lines (IC50 values of 68.75 and 69.77 μg mL−1 for AGS and A549, respectively).
29702573	0	15	theme	Agents	119:124	arg1	Producers					72:80	Producers	72:80	Producers of Anti-Cancer and Anti-Inflammatory Agents	72:124	Profiling of Heterobranchia Sea Slugs from Portuguese Coastal Waters as Producers of Anti-Cancer and Anti-Inflammatory Agents.
29702573	6	16	theme	levels	1206:1211	arg1	reduction					1190:1198	the significant reduction	1174:1198	the significant reduction of NO levels in LPS-challenged RAW 264.7 macrophages exposed to A. tricolorata extract	1174:1285	It is worth noting the significant reduction of NO levels in LPS-challenged RAW 264.7 macrophages exposed to A. tricolorata extract, at concentrations as low as 125 μg mL−1.
29702573	4	17	theme	fatty	761:765	arg1	profile					772:778	a fatty acid profile	759:778	a fatty acid profile	759:778	Qualitative and quantitative differences in the fatty acid content among species points to the existence of a fatty acid profile of neutral lipids, particularly of each genus.
29702573	2	18	dep	gastropods	407:416	arg1	maculata					425:432	Armina maculata	418:432	Armina maculata	418:432	Species that were not the object of studies on their biochemical composition include the Heterobranchia gastropods Armina maculata, Armina tigrina and Aglaja tricolorata, inhabitants of the Portuguese Atlantic coastal waters.
29702573	2	18	dep	gastropods	407:416	arg1	tigrina					442:448	Armina tigrina	435:448	Armina tigrina	435:448	Species that were not the object of studies on their biochemical composition include the Heterobranchia gastropods Armina maculata, Armina tigrina and Aglaja tricolorata, inhabitants of the Portuguese Atlantic coastal waters.
29702573	2	18	dep	gastropods	407:416	arg1	tricolorata					461:471	tricolorata	461:471	tricolorata	461:471	Species that were not the object of studies on their biochemical composition include the Heterobranchia gastropods Armina maculata, Armina tigrina and Aglaja tricolorata, inhabitants of the Portuguese Atlantic coastal waters.
29702573	0	19	theme	Anti-Inflammatory	101:117	arg1	Agents					119:124	Anti-Cancer and Anti-Inflammatory Agents	85:124	Anti-Cancer and Anti-Inflammatory Agents	85:124	Profiling of Heterobranchia Sea Slugs from Portuguese Coastal Waters as Producers of Anti-Cancer and Anti-Inflammatory Agents.
29702573	5	20	theme	IC50	1083:1086	arg1	values					1088:1093	IC50 values	1083:1093	IC50 values of 68.75 and 69.77 μg mL−1 for AGS and A549	1083:1137	The results from cytotoxicity assays, using the acetonic extracts of the gastropods on human gastric adenocarcinoma (AGS) and human lung adenocarcinoma (A549) cell lines, revealed a pronounced cytotoxic effect of the A. tigrina extract on both cell lines (IC50 values of 68.75 and 69.77 μg mL−1 for AGS and A549, respectively).
29702573	4	21	theme	lipids	791:796	arg1	profile					772:778	a fatty acid profile	759:778	a fatty acid profile	759:778	Qualitative and quantitative differences in the fatty acid content among species points to the existence of a fatty acid profile of neutral lipids, particularly of each genus.
29702573	5	22	theme	acetonic	875:882	arg1	extracts					884:891	the acetonic extracts	871:891	the acetonic extracts of the gastropods on human gastric adenocarcinoma (AGS) and human lung adenocarcinoma (A549) cell lines	871:995	The results from cytotoxicity assays, using the acetonic extracts of the gastropods on human gastric adenocarcinoma (AGS) and human lung adenocarcinoma (A549) cell lines, revealed a pronounced cytotoxic effect of the A. tigrina extract on both cell lines (IC50 values of 68.75 and 69.77 μg mL−1 for AGS and A549, respectively).
29702573	3	23	theme	first	554:558	arg1	time					560:563	the first time	550:563	the first time	550:563	Here, we present for the first time the fatty acid profile of neutral lipids and homarine content of these three species.
29702573	6	24	from	reduction	1190:1198	arg1	macrophages					1241:1251	LPS-challenged RAW 264.7 macrophages	1216:1251	LPS-challenged RAW 264.7 macrophages exposed to A. tricolorata extract	1216:1285	It is worth noting the significant reduction of NO levels in LPS-challenged RAW 264.7 macrophages exposed to A. tricolorata extract, at concentrations as low as 125 μg mL−1.
29702573	2	25	theme	studies	339:345	arg1	object					329:334	the object	325:334	the object of studies on their biochemical composition	325:378	Species that were not the object of studies on their biochemical composition include the Heterobranchia gastropods Armina maculata, Armina tigrina and Aglaja tricolorata, inhabitants of the Portuguese Atlantic coastal waters.
29702573	2	25	theme	studies	339:345	arg1	Species					303:309	Species	303:309	Species that were not the object of studies on their biochemical composition	303:378	Species that were not the object of studies on their biochemical composition include the Heterobranchia gastropods Armina maculata, Armina tigrina and Aglaja tricolorata, inhabitants of the Portuguese Atlantic coastal waters.
29702573	5	26	from	lines	991:995	arg1	extracts					884:891	the acetonic extracts	871:891	the acetonic extracts of the gastropods on human gastric adenocarcinoma (AGS) and human lung adenocarcinoma (A549) cell lines	871:995	The results from cytotoxicity assays, using the acetonic extracts of the gastropods on human gastric adenocarcinoma (AGS) and human lung adenocarcinoma (A549) cell lines, revealed a pronounced cytotoxic effect of the A. tigrina extract on both cell lines (IC50 values of 68.75 and 69.77 μg mL−1 for AGS and A549, respectively).
29702573	4	27	theme	acid	705:708	arg1	content					710:716	the fatty acid content	695:716	the fatty acid content	695:716	Qualitative and quantitative differences in the fatty acid content among species points to the existence of a fatty acid profile of neutral lipids, particularly of each genus.
29702573	3	28	theme	lipids	599:604	arg1	content					619:625	homarine content	610:625	homarine content of these three species	610:648	Here, we present for the first time the fatty acid profile of neutral lipids and homarine content of these three species.
29702573	3	28	theme	lipids	599:604	arg1	profile					580:586	the fatty acid profile	565:586	the fatty acid profile of neutral lipids	565:604	Here, we present for the first time the fatty acid profile of neutral lipids and homarine content of these three species.
29702573	4	29	from	differences	680:690	arg1	content					710:716	the fatty acid content	695:716	the fatty acid content	695:716	Qualitative and quantitative differences in the fatty acid content among species points to the existence of a fatty acid profile of neutral lipids, particularly of each genus.
29702573	1	30	theme	biological	203:212	arg1	richness					214:221	the biological richness	199:221	the biological richness of tropical regions	199:241	Bioprospection of marine invertebrates has been predominantly biased by the biological richness of tropical regions, thus neglecting macro-organisms from temperate ecosystems.
29702573	0	31	theme	Sea	28:30	arg1	Slugs					32:36	Heterobranchia Sea Slugs	13:36	Heterobranchia Sea Slugs from Portuguese Coastal Waters as Producers of Anti-Cancer and Anti-Inflammatory Agents	13:124	Profiling of Heterobranchia Sea Slugs from Portuguese Coastal Waters as Producers of Anti-Cancer and Anti-Inflammatory Agents.
29702573	1	32	from	ecosystems	291:300	arg1	macro-organisms					260:274	macro-organisms	260:274	macro-organisms from temperate ecosystems	260:300	Bioprospection of marine invertebrates has been predominantly biased by the biological richness of tropical regions, thus neglecting macro-organisms from temperate ecosystems.
29702573	5	33	from	adenocarcinoma	928:941	arg1	extracts					884:891	the acetonic extracts	871:891	the acetonic extracts of the gastropods on human gastric adenocarcinoma (AGS) and human lung adenocarcinoma (A549) cell lines	871:995	The results from cytotoxicity assays, using the acetonic extracts of the gastropods on human gastric adenocarcinoma (AGS) and human lung adenocarcinoma (A549) cell lines, revealed a pronounced cytotoxic effect of the A. tigrina extract on both cell lines (IC50 values of 68.75 and 69.77 μg mL−1 for AGS and A549, respectively).
29702573	4	34	theme	profile	772:778	arg1	existence					746:754	the existence	742:754	the existence of a fatty acid profile of neutral lipids, particularly of each genus	742:824	Qualitative and quantitative differences in the fatty acid content among species points to the existence of a fatty acid profile of neutral lipids, particularly of each genus.
29702573	5	35	from	effect	1030:1035	arg1	lines					1076:1080	both cell lines	1066:1080	both cell lines (IC50 values of 68.75 and 69.77 μg mL−1 for AGS and A549, respectively)	1066:1152	The results from cytotoxicity assays, using the acetonic extracts of the gastropods on human gastric adenocarcinoma (AGS) and human lung adenocarcinoma (A549) cell lines, revealed a pronounced cytotoxic effect of the A. tigrina extract on both cell lines (IC50 values of 68.75 and 69.77 μg mL−1 for AGS and A549, respectively).
29702573	5	36	theme	cytotoxicity	844:855	arg1	assays					857:862	cytotoxicity assays	844:862	cytotoxicity assays	844:862	The results from cytotoxicity assays, using the acetonic extracts of the gastropods on human gastric adenocarcinoma (AGS) and human lung adenocarcinoma (A549) cell lines, revealed a pronounced cytotoxic effect of the A. tigrina extract on both cell lines (IC50 values of 68.75 and 69.77 μg mL−1 for AGS and A549, respectively).
29702573	0	37	theme	Heterobranchia	13:26	arg1	Slugs					32:36	Heterobranchia Sea Slugs	13:36	Heterobranchia Sea Slugs from Portuguese Coastal Waters as Producers of Anti-Cancer and Anti-Inflammatory Agents	13:124	Profiling of Heterobranchia Sea Slugs from Portuguese Coastal Waters as Producers of Anti-Cancer and Anti-Inflammatory Agents.
29702573	5	38	theme	A549	980:983	arg1	lines					991:995	human lung adenocarcinoma (A549) cell lines	953:995	human lung adenocarcinoma (A549) cell lines	953:995	The results from cytotoxicity assays, using the acetonic extracts of the gastropods on human gastric adenocarcinoma (AGS) and human lung adenocarcinoma (A549) cell lines, revealed a pronounced cytotoxic effect of the A. tigrina extract on both cell lines (IC50 values of 68.75 and 69.77 μg mL−1 for AGS and A549, respectively).
29702573	5	39	theme	extract	1055:1061	arg1	effect					1030:1035	a pronounced cytotoxic effect	1007:1035	a pronounced cytotoxic effect of the A. tigrina extract on both cell lines (IC50 values of 68.75 and 69.77 μg mL−1 for AGS and A549, respectively)	1007:1152	The results from cytotoxicity assays, using the acetonic extracts of the gastropods on human gastric adenocarcinoma (AGS) and human lung adenocarcinoma (A549) cell lines, revealed a pronounced cytotoxic effect of the A. tigrina extract on both cell lines (IC50 values of 68.75 and 69.77 μg mL−1 for AGS and A549, respectively).
29702573	4	40	theme	acid	767:770	arg1	profile					772:778	a fatty acid profile	759:778	a fatty acid profile	759:778	Qualitative and quantitative differences in the fatty acid content among species points to the existence of a fatty acid profile of neutral lipids, particularly of each genus.
29702573	4	41	theme	Qualitative	651:661	arg1	differences					680:690	Qualitative and quantitative differences	651:690	Qualitative and quantitative differences in the fatty acid content among species	651:730	Qualitative and quantitative differences in the fatty acid content among species points to the existence of a fatty acid profile of neutral lipids, particularly of each genus.
29702573	2	42	theme	waters	521:526	arg1	inhabitants					474:484	inhabitants	474:484	inhabitants of the Portuguese Atlantic coastal waters	474:526	Species that were not the object of studies on their biochemical composition include the Heterobranchia gastropods Armina maculata, Armina tigrina and Aglaja tricolorata, inhabitants of the Portuguese Atlantic coastal waters.
29702573	2	42	theme	waters	521:526	arg1	gastropods					407:416	the Heterobranchia gastropods Armina maculata, Armina tigrina and Aglaja tricolorata	388:471	the Heterobranchia gastropods Armina maculata, Armina tigrina and Aglaja tricolorata	388:471	Species that were not the object of studies on their biochemical composition include the Heterobranchia gastropods Armina maculata, Armina tigrina and Aglaja tricolorata, inhabitants of the Portuguese Atlantic coastal waters.
29702573	5	43	theme	adenocarcinoma	964:977	arg1	lines					991:995	human lung adenocarcinoma (A549) cell lines	953:995	human lung adenocarcinoma (A549) cell lines	953:995	The results from cytotoxicity assays, using the acetonic extracts of the gastropods on human gastric adenocarcinoma (AGS) and human lung adenocarcinoma (A549) cell lines, revealed a pronounced cytotoxic effect of the A. tigrina extract on both cell lines (IC50 values of 68.75 and 69.77 μg mL−1 for AGS and A549, respectively).
29702573	6	44	theme	significant	1178:1188	arg1	reduction					1190:1198	the significant reduction	1174:1198	the significant reduction of NO levels in LPS-challenged RAW 264.7 macrophages exposed to A. tricolorata extract	1174:1285	It is worth noting the significant reduction of NO levels in LPS-challenged RAW 264.7 macrophages exposed to A. tricolorata extract, at concentrations as low as 125 μg mL−1.
29702573	4	45	theme	fatty	699:703	arg1	content					710:716	the fatty acid content	695:716	the fatty acid content	695:716	Qualitative and quantitative differences in the fatty acid content among species points to the existence of a fatty acid profile of neutral lipids, particularly of each genus.
29702573	1	46	theme	tropical	226:233	arg1	regions					235:241	tropical regions	226:241	tropical regions	226:241	Bioprospection of marine invertebrates has been predominantly biased by the biological richness of tropical regions, thus neglecting macro-organisms from temperate ecosystems.
29702573	0	47	theme	Slugs	32:36	arg1	Profiling					0:8	Profiling	0:8	Profiling of Heterobranchia Sea Slugs from Portuguese Coastal Waters as Producers of Anti-Cancer and Anti-Inflammatory Agents.	0:125	Profiling of Heterobranchia Sea Slugs from Portuguese Coastal Waters as Producers of Anti-Cancer and Anti-Inflammatory Agents.
29702573	5	48	theme	μg	1114:1115	arg1	mL−1					1117:1120	68.75 and 69.77 μg mL−1	1098:1120	68.75 and 69.77 μg mL−1	1098:1120	The results from cytotoxicity assays, using the acetonic extracts of the gastropods on human gastric adenocarcinoma (AGS) and human lung adenocarcinoma (A549) cell lines, revealed a pronounced cytotoxic effect of the A. tigrina extract on both cell lines (IC50 values of 68.75 and 69.77 μg mL−1 for AGS and A549, respectively).
29702573	2	49	theme	coastal	513:519	arg1	waters					521:526	the Portuguese Atlantic coastal waters	489:526	the Portuguese Atlantic coastal waters	489:526	Species that were not the object of studies on their biochemical composition include the Heterobranchia gastropods Armina maculata, Armina tigrina and Aglaja tricolorata, inhabitants of the Portuguese Atlantic coastal waters.
29702573	5	50	theme	pronounced	1009:1018	arg1	effect					1030:1035	a pronounced cytotoxic effect	1007:1035	a pronounced cytotoxic effect of the A. tigrina extract on both cell lines (IC50 values of 68.75 and 69.77 μg mL−1 for AGS and A549, respectively)	1007:1152	The results from cytotoxicity assays, using the acetonic extracts of the gastropods on human gastric adenocarcinoma (AGS) and human lung adenocarcinoma (A549) cell lines, revealed a pronounced cytotoxic effect of the A. tigrina extract on both cell lines (IC50 values of 68.75 and 69.77 μg mL−1 for AGS and A549, respectively).
29702573	1	51	theme	regions	235:241	arg1	richness					214:221	the biological richness	199:221	the biological richness of tropical regions	199:241	Bioprospection of marine invertebrates has been predominantly biased by the biological richness of tropical regions, thus neglecting macro-organisms from temperate ecosystems.
29702573	0	52	theme	Coastal	54:60	arg1	Waters					62:67	Portuguese Coastal Waters	43:67	Portuguese Coastal Waters as Producers of Anti-Cancer and Anti-Inflammatory Agents	43:124	Profiling of Heterobranchia Sea Slugs from Portuguese Coastal Waters as Producers of Anti-Cancer and Anti-Inflammatory Agents.
29702573	5	53	theme	mL−1	1117:1120	arg1	values					1088:1093	IC50 values	1083:1093	IC50 values of 68.75 and 69.77 μg mL−1 for AGS and A549	1083:1137	The results from cytotoxicity assays, using the acetonic extracts of the gastropods on human gastric adenocarcinoma (AGS) and human lung adenocarcinoma (A549) cell lines, revealed a pronounced cytotoxic effect of the A. tigrina extract on both cell lines (IC50 values of 68.75 and 69.77 μg mL−1 for AGS and A549, respectively).
29702573	6	54	theme	A.	1264:1265	arg1	extract					1279:1285	A. tricolorata extract	1264:1285	A. tricolorata extract	1264:1285	It is worth noting the significant reduction of NO levels in LPS-challenged RAW 264.7 macrophages exposed to A. tricolorata extract, at concentrations as low as 125 μg mL−1.
29702573	0	55	from	Waters	62:67	arg1	Profiling					0:8	Profiling	0:8	Profiling of Heterobranchia Sea Slugs from Portuguese Coastal Waters as Producers of Anti-Cancer and Anti-Inflammatory Agents.	0:125	Profiling of Heterobranchia Sea Slugs from Portuguese Coastal Waters as Producers of Anti-Cancer and Anti-Inflammatory Agents.
29702573	0	55	from	Waters	62:67	arg1	Slugs					32:36	Heterobranchia Sea Slugs	13:36	Heterobranchia Sea Slugs from Portuguese Coastal Waters as Producers of Anti-Cancer and Anti-Inflammatory Agents	13:124	Profiling of Heterobranchia Sea Slugs from Portuguese Coastal Waters as Producers of Anti-Cancer and Anti-Inflammatory Agents.
29702573	5	56	theme	cytotoxic	1020:1028	arg1	effect					1030:1035	a pronounced cytotoxic effect	1007:1035	a pronounced cytotoxic effect of the A. tigrina extract on both cell lines (IC50 values of 68.75 and 69.77 μg mL−1 for AGS and A549, respectively)	1007:1152	The results from cytotoxicity assays, using the acetonic extracts of the gastropods on human gastric adenocarcinoma (AGS) and human lung adenocarcinoma (A549) cell lines, revealed a pronounced cytotoxic effect of the A. tigrina extract on both cell lines (IC50 values of 68.75 and 69.77 μg mL−1 for AGS and A549, respectively).
29702573	5	57	theme	gastric	920:926	arg1	adenocarcinoma					928:941	human gastric adenocarcinoma	914:941	human gastric adenocarcinoma (AGS)	914:947	The results from cytotoxicity assays, using the acetonic extracts of the gastropods on human gastric adenocarcinoma (AGS) and human lung adenocarcinoma (A549) cell lines, revealed a pronounced cytotoxic effect of the A. tigrina extract on both cell lines (IC50 values of 68.75 and 69.77 μg mL−1 for AGS and A549, respectively).
29702573	5	57	theme	gastric	920:926	arg1	AGS					944:946	AGS	944:946	AGS	944:946	The results from cytotoxicity assays, using the acetonic extracts of the gastropods on human gastric adenocarcinoma (AGS) and human lung adenocarcinoma (A549) cell lines, revealed a pronounced cytotoxic effect of the A. tigrina extract on both cell lines (IC50 values of 68.75 and 69.77 μg mL−1 for AGS and A549, respectively).
29702573	0	58	theme	Portuguese	43:52	arg1	Waters					62:67	Portuguese Coastal Waters	43:67	Portuguese Coastal Waters as Producers of Anti-Cancer and Anti-Inflammatory Agents	43:124	Profiling of Heterobranchia Sea Slugs from Portuguese Coastal Waters as Producers of Anti-Cancer and Anti-Inflammatory Agents.
29702573	5	59	dep	lines	1076:1080	arg1	values					1088:1093	IC50 values	1083:1093	IC50 values of 68.75 and 69.77 μg mL−1 for AGS and A549	1083:1137	The results from cytotoxicity assays, using the acetonic extracts of the gastropods on human gastric adenocarcinoma (AGS) and human lung adenocarcinoma (A549) cell lines, revealed a pronounced cytotoxic effect of the A. tigrina extract on both cell lines (IC50 values of 68.75 and 69.77 μg mL−1 for AGS and A549, respectively).
29702573	2	60	theme	Portuguese	493:502	arg1	waters					521:526	the Portuguese Atlantic coastal waters	489:526	the Portuguese Atlantic coastal waters	489:526	Species that were not the object of studies on their biochemical composition include the Heterobranchia gastropods Armina maculata, Armina tigrina and Aglaja tricolorata, inhabitants of the Portuguese Atlantic coastal waters.
29702573	3	61	theme	homarine	610:617	arg1	content					619:625	homarine content	610:625	homarine content of these three species	610:648	Here, we present for the first time the fatty acid profile of neutral lipids and homarine content of these three species.
29702573	2	62	theme	biochemical	356:366	arg1	composition					368:378	their biochemical composition	350:378	their biochemical composition	350:378	Species that were not the object of studies on their biochemical composition include the Heterobranchia gastropods Armina maculata, Armina tigrina and Aglaja tricolorata, inhabitants of the Portuguese Atlantic coastal waters.
29702573	6	63	from	macrophages	1241:1251	arg1	reduction					1190:1198	the significant reduction	1174:1198	the significant reduction of NO levels in LPS-challenged RAW 264.7 macrophages exposed to A. tricolorata extract	1174:1285	It is worth noting the significant reduction of NO levels in LPS-challenged RAW 264.7 macrophages exposed to A. tricolorata extract, at concentrations as low as 125 μg mL−1.
29702573	3	64	theme	neutral	591:597	arg1	lipids					599:604	neutral lipids	591:604	neutral lipids	591:604	Here, we present for the first time the fatty acid profile of neutral lipids and homarine content of these three species.
29702573	5	65	theme	cell	986:989	arg1	lines					991:995	human lung adenocarcinoma (A549) cell lines	953:995	human lung adenocarcinoma (A549) cell lines	953:995	The results from cytotoxicity assays, using the acetonic extracts of the gastropods on human gastric adenocarcinoma (AGS) and human lung adenocarcinoma (A549) cell lines, revealed a pronounced cytotoxic effect of the A. tigrina extract on both cell lines (IC50 values of 68.75 and 69.77 μg mL−1 for AGS and A549, respectively).
29702573	3	66	theme	species	642:648	arg1	content					619:625	homarine content	610:625	homarine content of these three species	610:648	Here, we present for the first time the fatty acid profile of neutral lipids and homarine content of these three species.
29702573	3	66	theme	species	642:648	arg1	profile					580:586	the fatty acid profile	565:586	the fatty acid profile of neutral lipids	565:604	Here, we present for the first time the fatty acid profile of neutral lipids and homarine content of these three species.
29702573	5	67	from	assays	857:862	arg1	results					831:837	The results	827:837	The results from cytotoxicity assays, using the acetonic extracts of the gastropods on human gastric adenocarcinoma (AGS) and human lung adenocarcinoma (A549) cell lines,	827:996	The results from cytotoxicity assays, using the acetonic extracts of the gastropods on human gastric adenocarcinoma (AGS) and human lung adenocarcinoma (A549) cell lines, revealed a pronounced cytotoxic effect of the A. tigrina extract on both cell lines (IC50 values of 68.75 and 69.77 μg mL−1 for AGS and A549, respectively).
29702573	5	68	theme	human	914:918	arg1	adenocarcinoma					928:941	human gastric adenocarcinoma	914:941	human gastric adenocarcinoma (AGS)	914:947	The results from cytotoxicity assays, using the acetonic extracts of the gastropods on human gastric adenocarcinoma (AGS) and human lung adenocarcinoma (A549) cell lines, revealed a pronounced cytotoxic effect of the A. tigrina extract on both cell lines (IC50 values of 68.75 and 69.77 μg mL−1 for AGS and A549, respectively).
29702573	5	68	theme	human	914:918	arg1	AGS					944:946	AGS	944:946	AGS	944:946	The results from cytotoxicity assays, using the acetonic extracts of the gastropods on human gastric adenocarcinoma (AGS) and human lung adenocarcinoma (A549) cell lines, revealed a pronounced cytotoxic effect of the A. tigrina extract on both cell lines (IC50 values of 68.75 and 69.77 μg mL−1 for AGS and A549, respectively).
29702573	5	69	theme	human	953:957	arg1	lines					991:995	human lung adenocarcinoma (A549) cell lines	953:995	human lung adenocarcinoma (A549) cell lines	953:995	The results from cytotoxicity assays, using the acetonic extracts of the gastropods on human gastric adenocarcinoma (AGS) and human lung adenocarcinoma (A549) cell lines, revealed a pronounced cytotoxic effect of the A. tigrina extract on both cell lines (IC50 values of 68.75 and 69.77 μg mL−1 for AGS and A549, respectively).
29702573	6	70	theme	tricolorata	1267:1277	arg1	extract					1279:1285	A. tricolorata extract	1264:1285	A. tricolorata extract	1264:1285	It is worth noting the significant reduction of NO levels in LPS-challenged RAW 264.7 macrophages exposed to A. tricolorata extract, at concentrations as low as 125 μg mL−1.
29702573	5	71	theme	A.	1044:1045	arg1	extract					1055:1061	the A. tigrina extract	1040:1061	the A. tigrina extract	1040:1061	The results from cytotoxicity assays, using the acetonic extracts of the gastropods on human gastric adenocarcinoma (AGS) and human lung adenocarcinoma (A549) cell lines, revealed a pronounced cytotoxic effect of the A. tigrina extract on both cell lines (IC50 values of 68.75 and 69.77 μg mL−1 for AGS and A549, respectively).
29702573	4	72	theme	neutral	783:789	arg1	lipids					791:796	neutral lipids	783:796	neutral lipids	783:796	Qualitative and quantitative differences in the fatty acid content among species points to the existence of a fatty acid profile of neutral lipids, particularly of each genus.
29702573	5	73	from	extracts	884:891	arg1	adenocarcinoma					928:941	human gastric adenocarcinoma	914:941	human gastric adenocarcinoma (AGS)	914:947	The results from cytotoxicity assays, using the acetonic extracts of the gastropods on human gastric adenocarcinoma (AGS) and human lung adenocarcinoma (A549) cell lines, revealed a pronounced cytotoxic effect of the A. tigrina extract on both cell lines (IC50 values of 68.75 and 69.77 μg mL−1 for AGS and A549, respectively).
29702573	5	73	from	extracts	884:891	arg1	lines					991:995	human lung adenocarcinoma (A549) cell lines	953:995	human lung adenocarcinoma (A549) cell lines	953:995	The results from cytotoxicity assays, using the acetonic extracts of the gastropods on human gastric adenocarcinoma (AGS) and human lung adenocarcinoma (A549) cell lines, revealed a pronounced cytotoxic effect of the A. tigrina extract on both cell lines (IC50 values of 68.75 and 69.77 μg mL−1 for AGS and A549, respectively).
29702573	5	73	from	extracts	884:891	arg1	AGS					944:946	AGS	944:946	AGS	944:946	The results from cytotoxicity assays, using the acetonic extracts of the gastropods on human gastric adenocarcinoma (AGS) and human lung adenocarcinoma (A549) cell lines, revealed a pronounced cytotoxic effect of the A. tigrina extract on both cell lines (IC50 values of 68.75 and 69.77 μg mL−1 for AGS and A549, respectively).
29702573	1	74	theme	marine	145:150	arg1	invertebrates					152:164	marine invertebrates	145:164	marine invertebrates	145:164	Bioprospection of marine invertebrates has been predominantly biased by the biological richness of tropical regions, thus neglecting macro-organisms from temperate ecosystems.
29702573	4	75	theme	quantitative	667:678	arg1	differences					680:690	Qualitative and quantitative differences	651:690	Qualitative and quantitative differences in the fatty acid content among species	651:730	Qualitative and quantitative differences in the fatty acid content among species points to the existence of a fatty acid profile of neutral lipids, particularly of each genus.
29702573	4	76	theme	genus	820:824	arg1	profile					772:778	a fatty acid profile	759:778	a fatty acid profile	759:778	Qualitative and quantitative differences in the fatty acid content among species points to the existence of a fatty acid profile of neutral lipids, particularly of each genus.
29702573	1	77	theme	invertebrates	152:164	arg1	Bioprospection					127:140	Bioprospection	127:140	Bioprospection of marine invertebrates	127:164	Bioprospection of marine invertebrates has been predominantly biased by the biological richness of tropical regions, thus neglecting macro-organisms from temperate ecosystems.
29702573	2	78	theme	Atlantic	504:511	arg1	waters					521:526	the Portuguese Atlantic coastal waters	489:526	the Portuguese Atlantic coastal waters	489:526	Species that were not the object of studies on their biochemical composition include the Heterobranchia gastropods Armina maculata, Armina tigrina and Aglaja tricolorata, inhabitants of the Portuguese Atlantic coastal waters.
29702573	2	79	theme	Heterobranchia	392:405	arg1	inhabitants					474:484	inhabitants	474:484	inhabitants of the Portuguese Atlantic coastal waters	474:526	Species that were not the object of studies on their biochemical composition include the Heterobranchia gastropods Armina maculata, Armina tigrina and Aglaja tricolorata, inhabitants of the Portuguese Atlantic coastal waters.
29702573	2	79	theme	Heterobranchia	392:405	arg1	gastropods					407:416	the Heterobranchia gastropods Armina maculata, Armina tigrina and Aglaja tricolorata	388:471	the Heterobranchia gastropods Armina maculata, Armina tigrina and Aglaja tricolorata	388:471	Species that were not the object of studies on their biochemical composition include the Heterobranchia gastropods Armina maculata, Armina tigrina and Aglaja tricolorata, inhabitants of the Portuguese Atlantic coastal waters.
29702573	2	80	from	object	329:334	arg1	composition					368:378	their biochemical composition	350:378	their biochemical composition	350:378	Species that were not the object of studies on their biochemical composition include the Heterobranchia gastropods Armina maculata, Armina tigrina and Aglaja tricolorata, inhabitants of the Portuguese Atlantic coastal waters.
29702573	6	81	theme	low	1309:1311	arg1	concentrations					1291:1304	concentrations	1291:1304	concentrations as low as 125 μg mL−1	1291:1326	It is worth noting the significant reduction of NO levels in LPS-challenged RAW 264.7 macrophages exposed to A. tricolorata extract, at concentrations as low as 125 μg mL−1.
31568499	8	0	theme	intestinal	1109:1118	arg1	equilibrium					1120:1130	intestinal equilibrium	1109:1130	intestinal equilibrium	1109:1130	Moreover, changing of some specific microbial groups such as Proteobacteria and Bacteroides was closely correlated with the re-establishment of intestinal equilibrium and the recovery of intestinal function.
31568499	10	1	theme	colitis	1458:1464	arg1	therapy					1466:1472	colitis therapy	1458:1472	colitis therapy	1458:1472	The current study provides additional mechanistic insight in the therapeutic effect of cinobufacini treatment and may pave the way for clinical application of cinobufacini in colitis therapy.
31568499	7	2	theme	intestinal	935:944	arg1	integrity					954:962	intestinal barrier integrity	935:962	intestinal barrier integrity	935:962	RESULTS Our data indicated that cinobufacini reversed DSS-induced gut dysbiosis and enhanced intestinal barrier integrity.
31568499	1	3	theme	toad	166:169	arg1	skin					171:174	dried toad skin	160:174	dried toad skin	160:174	BACKGROUND Cinobufacini, the sterilized hot water extraction of dried toad skin, has been widely used in the treatment of inflammation and cancers.
31568499	4	4	theme	C57BL/6	564:570	arg1	mice					572:575	METHODS C57BL/6 mice	556:575	METHODS C57BL/6 mice	556:575	METHODS C57BL/6 mice were supplied with normal or 3.0% DSS containing drinking water.
31568499	0	5	theme	microbiota	84:93	arg1	composition					65:75	the composition	61:75	the composition of gut microbiota	61:93	Cinobufacini ameliorates experimental colitis via modulating the composition of gut microbiota.
31568499	1	6	theme	skin	171:174	arg1	Cinobufacini					107:118	BACKGROUND Cinobufacini	96:118	BACKGROUND Cinobufacini	96:118	BACKGROUND Cinobufacini, the sterilized hot water extraction of dried toad skin, has been widely used in the treatment of inflammation and cancers.
31568499	1	6	theme	skin	171:174	arg1	extraction					146:155	the sterilized hot water extraction	121:155	the sterilized hot water extraction of dried toad skin	121:174	BACKGROUND Cinobufacini, the sterilized hot water extraction of dried toad skin, has been widely used in the treatment of inflammation and cancers.
31568499	4	7	theme	METHODS	556:562	arg1	mice					572:575	METHODS C57BL/6 mice	556:575	METHODS C57BL/6 mice	556:575	METHODS C57BL/6 mice were supplied with normal or 3.0% DSS containing drinking water.
31568499	10	8	theme	clinical	1418:1425	arg1	application					1427:1437	clinical application	1418:1437	clinical application of cinobufacini in colitis therapy	1418:1472	The current study provides additional mechanistic insight in the therapeutic effect of cinobufacini treatment and may pave the way for clinical application of cinobufacini in colitis therapy.
31568499	8	9	theme	microbial	1001:1009	arg1	Bacteroides					1045:1055	Bacteroides	1045:1055	Bacteroides	1045:1055	Moreover, changing of some specific microbial groups such as Proteobacteria and Bacteroides was closely correlated with the re-establishment of intestinal equilibrium and the recovery of intestinal function.
31568499	8	9	theme	microbial	1001:1009	arg1	groups					1011:1016	some specific microbial groups	987:1016	some specific microbial groups such as Proteobacteria and Bacteroides	987:1055	Moreover, changing of some specific microbial groups such as Proteobacteria and Bacteroides was closely correlated with the re-establishment of intestinal equilibrium and the recovery of intestinal function.
31568499	8	9	theme	microbial	1001:1009	arg1	Proteobacteria					1026:1039	Proteobacteria	1026:1039	Proteobacteria	1026:1039	Moreover, changing of some specific microbial groups such as Proteobacteria and Bacteroides was closely correlated with the re-establishment of intestinal equilibrium and the recovery of intestinal function.
31568499	3	10	theme	contents	546:553	arg1	pyrosequencing					520:533	pyrosequencing	520:533	pyrosequencing of colonic contents	520:553	In current study, we explored the effect of cinobufacini on gut microbiota in DSS-induced acute colitic mouse model by pyrosequencing of colonic contents.
31568499	5	11	theme	DSS-treat	642:650	arg1	mice					652:655	DSS-treat mice	642:655	DSS-treat mice	642:655	DSS-treat mice were gavaged daily either with vehicle (water) or cinobufacini (10.0 or 30.0 mg/kg) for 7 days.
31568499	7	12	theme	DSS-induced	896:906	arg1	dysbiosis					912:920	DSS-induced gut dysbiosis	896:920	DSS-induced gut dysbiosis	896:920	RESULTS Our data indicated that cinobufacini reversed DSS-induced gut dysbiosis and enhanced intestinal barrier integrity.
31568499	10	13	from	application	1427:1437	arg1	therapy					1466:1472	colitis therapy	1458:1472	colitis therapy	1458:1472	The current study provides additional mechanistic insight in the therapeutic effect of cinobufacini treatment and may pave the way for clinical application of cinobufacini in colitis therapy.
31568499	10	14	theme	current	1287:1293	arg1	study					1295:1299	The current study	1283:1299	The current study	1283:1299	The current study provides additional mechanistic insight in the therapeutic effect of cinobufacini treatment and may pave the way for clinical application of cinobufacini in colitis therapy.
31568499	9	15	from	colitis	1206:1212	arg1	mice					1217:1220	mice	1217:1220	mice	1217:1220	CONCLUSION Cinobufacini prevents colitis in mice by modifying the composition and function of gut microbiota.
31568499	8	16	theme	intestinal	1152:1161	arg1	function					1163:1170	intestinal function	1152:1170	intestinal function	1152:1170	Moreover, changing of some specific microbial groups such as Proteobacteria and Bacteroides was closely correlated with the re-establishment of intestinal equilibrium and the recovery of intestinal function.
31568499	1	17	theme	sterilized	125:134	arg1	Cinobufacini					107:118	BACKGROUND Cinobufacini	96:118	BACKGROUND Cinobufacini	96:118	BACKGROUND Cinobufacini, the sterilized hot water extraction of dried toad skin, has been widely used in the treatment of inflammation and cancers.
31568499	1	17	theme	sterilized	125:134	arg1	extraction					146:155	the sterilized hot water extraction	121:155	the sterilized hot water extraction of dried toad skin	121:174	BACKGROUND Cinobufacini, the sterilized hot water extraction of dried toad skin, has been widely used in the treatment of inflammation and cancers.
31568499	2	18	from	colitis	329:335	arg1	mice					340:343	mice	340:343	mice	340:343	Recently we found cinobufacini could ameliorate dextran sulfate sodium (DSS)-induced colitis in mice, but the underlying mechanism was not fully understood.
31568499	3	19	theme	mouse	505:509	arg1	model					511:515	DSS-induced acute colitic mouse model	479:515	DSS-induced acute colitic mouse model	479:515	In current study, we explored the effect of cinobufacini on gut microbiota in DSS-induced acute colitic mouse model by pyrosequencing of colonic contents.
31568499	6	20	theme	rRNA	821:824	arg1	sequences					831:839	16S rRNA gene sequences	817:839	16S rRNA gene sequences	817:839	The composition of the gut microbiota was assessed by analyzing 16S rRNA gene sequences.
31568499	1	21	theme	inflammation	218:229	arg1	treatment					205:213	the treatment	201:213	the treatment of inflammation and cancers	201:241	BACKGROUND Cinobufacini, the sterilized hot water extraction of dried toad skin, has been widely used in the treatment of inflammation and cancers.
31568499	4	22	theme	drinking	626:633	arg1	water					635:639	drinking water	626:639	drinking water	626:639	METHODS C57BL/6 mice were supplied with normal or 3.0% DSS containing drinking water.
31568499	6	23	theme	16S	817:819	arg1	sequences					831:839	16S rRNA gene sequences	817:839	16S rRNA gene sequences	817:839	The composition of the gut microbiota was assessed by analyzing 16S rRNA gene sequences.
31568499	8	24	theme	specific	992:999	arg1	Bacteroides					1045:1055	Bacteroides	1045:1055	Bacteroides	1045:1055	Moreover, changing of some specific microbial groups such as Proteobacteria and Bacteroides was closely correlated with the re-establishment of intestinal equilibrium and the recovery of intestinal function.
31568499	8	24	theme	specific	992:999	arg1	groups					1011:1016	some specific microbial groups	987:1016	some specific microbial groups such as Proteobacteria and Bacteroides	987:1055	Moreover, changing of some specific microbial groups such as Proteobacteria and Bacteroides was closely correlated with the re-establishment of intestinal equilibrium and the recovery of intestinal function.
31568499	8	24	theme	specific	992:999	arg1	Proteobacteria					1026:1039	Proteobacteria	1026:1039	Proteobacteria	1026:1039	Moreover, changing of some specific microbial groups such as Proteobacteria and Bacteroides was closely correlated with the re-establishment of intestinal equilibrium and the recovery of intestinal function.
31568499	5	25	dep	vehicle	688:694	arg1	either					676:681	either	676:681	either	676:681	DSS-treat mice were gavaged daily either with vehicle (water) or cinobufacini (10.0 or 30.0 mg/kg) for 7 days.
31568499	1	26	theme	BACKGROUND	96:105	arg1	Cinobufacini					107:118	BACKGROUND Cinobufacini	96:118	BACKGROUND Cinobufacini	96:118	BACKGROUND Cinobufacini, the sterilized hot water extraction of dried toad skin, has been widely used in the treatment of inflammation and cancers.
31568499	1	26	theme	BACKGROUND	96:105	arg1	extraction					146:155	the sterilized hot water extraction	121:155	the sterilized hot water extraction of dried toad skin	121:174	BACKGROUND Cinobufacini, the sterilized hot water extraction of dried toad skin, has been widely used in the treatment of inflammation and cancers.
31568499	6	27	theme	gene	826:829	arg1	sequences					831:839	16S rRNA gene sequences	817:839	16S rRNA gene sequences	817:839	The composition of the gut microbiota was assessed by analyzing 16S rRNA gene sequences.
31568499	3	28	theme	current	404:410	arg1	study					412:416	current study	404:416	current study	404:416	In current study, we explored the effect of cinobufacini on gut microbiota in DSS-induced acute colitic mouse model by pyrosequencing of colonic contents.
31568499	8	29	theme	function	1163:1170	arg1	recovery					1140:1147	the recovery	1136:1147	the recovery of intestinal function	1136:1170	Moreover, changing of some specific microbial groups such as Proteobacteria and Bacteroides was closely correlated with the re-establishment of intestinal equilibrium and the recovery of intestinal function.
31568499	8	29	theme	function	1163:1170	arg1	re-establishment					1089:1104	the re-establishment	1085:1104	the re-establishment of intestinal equilibrium	1085:1130	Moreover, changing of some specific microbial groups such as Proteobacteria and Bacteroides was closely correlated with the re-establishment of intestinal equilibrium and the recovery of intestinal function.
31568499	10	30	from	cinobufacini	1442:1453	arg1	therapy					1466:1472	colitis therapy	1458:1472	colitis therapy	1458:1472	The current study provides additional mechanistic insight in the therapeutic effect of cinobufacini treatment and may pave the way for clinical application of cinobufacini in colitis therapy.
31568499	10	31	theme	mechanistic	1321:1331	arg1	insight					1333:1339	additional mechanistic insight	1310:1339	additional mechanistic insight in the therapeutic effect of cinobufacini treatment	1310:1391	The current study provides additional mechanistic insight in the therapeutic effect of cinobufacini treatment and may pave the way for clinical application of cinobufacini in colitis therapy.
31568499	2	32	theme	underlying	354:363	arg1	mechanism					365:373	the underlying mechanism	350:373	the underlying mechanism	350:373	Recently we found cinobufacini could ameliorate dextran sulfate sodium (DSS)-induced colitis in mice, but the underlying mechanism was not fully understood.
31568499	10	33	theme	cinobufacini	1442:1453	arg1	application					1427:1437	clinical application	1418:1437	clinical application of cinobufacini in colitis therapy	1418:1472	The current study provides additional mechanistic insight in the therapeutic effect of cinobufacini treatment and may pave the way for clinical application of cinobufacini in colitis therapy.
31568499	7	34	theme	gut	908:910	arg1	dysbiosis					912:920	DSS-induced gut dysbiosis	896:920	DSS-induced gut dysbiosis	896:920	RESULTS Our data indicated that cinobufacini reversed DSS-induced gut dysbiosis and enhanced intestinal barrier integrity.
31568499	10	35	theme	additional	1310:1319	arg1	insight					1333:1339	additional mechanistic insight	1310:1339	additional mechanistic insight in the therapeutic effect of cinobufacini treatment	1310:1391	The current study provides additional mechanistic insight in the therapeutic effect of cinobufacini treatment and may pave the way for clinical application of cinobufacini in colitis therapy.
31568499	4	36	theme	%	609:609	arg1	DSS					611:613	normal or 3.0% DSS	596:613	normal or 3.0% DSS containing drinking water	596:639	METHODS C57BL/6 mice were supplied with normal or 3.0% DSS containing drinking water.
31568499	0	37	theme	experimental	25:36	arg1	colitis					38:44	experimental colitis	25:44	experimental colitis	25:44	Cinobufacini ameliorates experimental colitis via modulating the composition of gut microbiota.
31568499	3	38	from	effect	435:440	arg1	microbiota					465:474	gut microbiota	461:474	gut microbiota	461:474	In current study, we explored the effect of cinobufacini on gut microbiota in DSS-induced acute colitic mouse model by pyrosequencing of colonic contents.
31568499	3	39	theme	colonic	538:544	arg1	contents					546:553	colonic contents	538:553	colonic contents	538:553	In current study, we explored the effect of cinobufacini on gut microbiota in DSS-induced acute colitic mouse model by pyrosequencing of colonic contents.
31568499	4	40	contain	containing	615:624	arg1	DSS					611:613	normal or 3.0% DSS	596:613	normal or 3.0% DSS containing drinking water	596:639	METHODS C57BL/6 mice were supplied with normal or 3.0% DSS containing drinking water.
31568499	4	40	contain	containing	615:624	arg2	water					635:639	drinking water	626:639	drinking water	626:639	METHODS C57BL/6 mice were supplied with normal or 3.0% DSS containing drinking water.
31568499	8	41	theme	equilibrium	1120:1130	arg1	recovery					1140:1147	the recovery	1136:1147	the recovery of intestinal function	1136:1170	Moreover, changing of some specific microbial groups such as Proteobacteria and Bacteroides was closely correlated with the re-establishment of intestinal equilibrium and the recovery of intestinal function.
31568499	8	41	theme	equilibrium	1120:1130	arg1	re-establishment					1089:1104	the re-establishment	1085:1104	the re-establishment of intestinal equilibrium	1085:1130	Moreover, changing of some specific microbial groups such as Proteobacteria and Bacteroides was closely correlated with the re-establishment of intestinal equilibrium and the recovery of intestinal function.
31568499	10	42	theme	therapeutic	1348:1358	arg1	effect					1360:1365	the therapeutic effect	1344:1365	the therapeutic effect of cinobufacini treatment	1344:1391	The current study provides additional mechanistic insight in the therapeutic effect of cinobufacini treatment and may pave the way for clinical application of cinobufacini in colitis therapy.
31568499	10	43	from	therapy	1466:1472	arg1	application					1427:1437	clinical application	1418:1437	clinical application of cinobufacini in colitis therapy	1418:1472	The current study provides additional mechanistic insight in the therapeutic effect of cinobufacini treatment and may pave the way for clinical application of cinobufacini in colitis therapy.
31568499	10	44	theme	treatment	1383:1391	arg1	effect					1360:1365	the therapeutic effect	1344:1365	the therapeutic effect of cinobufacini treatment	1344:1391	The current study provides additional mechanistic insight in the therapeutic effect of cinobufacini treatment and may pave the way for clinical application of cinobufacini in colitis therapy.
31568499	3	45	theme	gut	461:463	arg1	microbiota					465:474	gut microbiota	461:474	gut microbiota	461:474	In current study, we explored the effect of cinobufacini on gut microbiota in DSS-induced acute colitic mouse model by pyrosequencing of colonic contents.
31568499	3	46	theme	cinobufacini	445:456	arg1	effect					435:440	the effect	431:440	the effect of cinobufacini on gut microbiota	431:474	In current study, we explored the effect of cinobufacini on gut microbiota in DSS-induced acute colitic mouse model by pyrosequencing of colonic contents.
31568499	6	47	theme	microbiota	780:789	arg1	composition					757:767	The composition	753:767	The composition of the gut microbiota	753:789	The composition of the gut microbiota was assessed by analyzing 16S rRNA gene sequences.
31568499	1	48	used	used	193:196	arg2	Cinobufacini					107:118	BACKGROUND Cinobufacini	96:118	BACKGROUND Cinobufacini	96:118	BACKGROUND Cinobufacini, the sterilized hot water extraction of dried toad skin, has been widely used in the treatment of inflammation and cancers.
31568499	1	48	used	used	193:196	arg2	extraction					146:155	the sterilized hot water extraction	121:155	the sterilized hot water extraction of dried toad skin	121:174	BACKGROUND Cinobufacini, the sterilized hot water extraction of dried toad skin, has been widely used in the treatment of inflammation and cancers.
31568499	3	49	theme	DSS-induced	479:489	arg1	model					511:515	DSS-induced acute colitic mouse model	479:515	DSS-induced acute colitic mouse model	479:515	In current study, we explored the effect of cinobufacini on gut microbiota in DSS-induced acute colitic mouse model by pyrosequencing of colonic contents.
31568499	1	50	theme	hot	136:138	arg1	Cinobufacini					107:118	BACKGROUND Cinobufacini	96:118	BACKGROUND Cinobufacini	96:118	BACKGROUND Cinobufacini, the sterilized hot water extraction of dried toad skin, has been widely used in the treatment of inflammation and cancers.
31568499	1	50	theme	hot	136:138	arg1	extraction					146:155	the sterilized hot water extraction	121:155	the sterilized hot water extraction of dried toad skin	121:174	BACKGROUND Cinobufacini, the sterilized hot water extraction of dried toad skin, has been widely used in the treatment of inflammation and cancers.
31568499	6	51	theme	gut	776:778	arg1	microbiota					780:789	the gut microbiota	772:789	the gut microbiota	772:789	The composition of the gut microbiota was assessed by analyzing 16S rRNA gene sequences.
31568499	1	52	theme	water	140:144	arg1	Cinobufacini					107:118	BACKGROUND Cinobufacini	96:118	BACKGROUND Cinobufacini	96:118	BACKGROUND Cinobufacini, the sterilized hot water extraction of dried toad skin, has been widely used in the treatment of inflammation and cancers.
31568499	1	52	theme	water	140:144	arg1	extraction					146:155	the sterilized hot water extraction	121:155	the sterilized hot water extraction of dried toad skin	121:174	BACKGROUND Cinobufacini, the sterilized hot water extraction of dried toad skin, has been widely used in the treatment of inflammation and cancers.
31568499	9	53	dep	composition	1239:1249	arg1	the					1235:1237	the	1235:1237	the	1235:1237	CONCLUSION Cinobufacini prevents colitis in mice by modifying the composition and function of gut microbiota.
31568499	7	54	theme	barrier	946:952	arg1	integrity					954:962	intestinal barrier integrity	935:962	intestinal barrier integrity	935:962	RESULTS Our data indicated that cinobufacini reversed DSS-induced gut dysbiosis and enhanced intestinal barrier integrity.
31568499	3	55	theme	colitic	497:503	arg1	model					511:515	DSS-induced acute colitic mouse model	479:515	DSS-induced acute colitic mouse model	479:515	In current study, we explored the effect of cinobufacini on gut microbiota in DSS-induced acute colitic mouse model by pyrosequencing of colonic contents.
31568499	2	56	theme	-induced	320:327	arg1	colitis					329:335	dextran sulfate sodium (DSS)-induced colitis	292:335	dextran sulfate sodium (DSS)-induced colitis in mice	292:343	Recently we found cinobufacini could ameliorate dextran sulfate sodium (DSS)-induced colitis in mice, but the underlying mechanism was not fully understood.
31568499	0	57	theme	gut	80:82	arg1	microbiota					84:93	gut microbiota	80:93	gut microbiota	80:93	Cinobufacini ameliorates experimental colitis via modulating the composition of gut microbiota.
31568499	9	58	theme	gut	1267:1269	arg1	microbiota					1271:1280	gut microbiota	1267:1280	gut microbiota	1267:1280	CONCLUSION Cinobufacini prevents colitis in mice by modifying the composition and function of gut microbiota.
31568499	10	59	theme	cinobufacini	1370:1381	arg1	treatment					1383:1391	cinobufacini treatment	1370:1391	cinobufacini treatment	1370:1391	The current study provides additional mechanistic insight in the therapeutic effect of cinobufacini treatment and may pave the way for clinical application of cinobufacini in colitis therapy.
31568499	3	60	theme	acute	491:495	arg1	model					511:515	DSS-induced acute colitic mouse model	479:515	DSS-induced acute colitic mouse model	479:515	In current study, we explored the effect of cinobufacini on gut microbiota in DSS-induced acute colitic mouse model by pyrosequencing of colonic contents.
31568499	10	61	from	insight	1333:1339	arg1	effect					1360:1365	the therapeutic effect	1344:1365	the therapeutic effect of cinobufacini treatment	1344:1391	The current study provides additional mechanistic insight in the therapeutic effect of cinobufacini treatment and may pave the way for clinical application of cinobufacini in colitis therapy.
31568499	9	62	theme	microbiota	1271:1280	arg1	function					1255:1262	function	1255:1262	function	1255:1262	CONCLUSION Cinobufacini prevents colitis in mice by modifying the composition and function of gut microbiota.
31568499	9	62	theme	microbiota	1271:1280	arg1	composition					1239:1249	composition	1239:1249	composition	1239:1249	CONCLUSION Cinobufacini prevents colitis in mice by modifying the composition and function of gut microbiota.
31568499	4	63	theme	normal	596:601	arg1	DSS					611:613	normal or 3.0% DSS	596:613	normal or 3.0% DSS containing drinking water	596:639	METHODS C57BL/6 mice were supplied with normal or 3.0% DSS containing drinking water.
31568499	1	64	theme	dried	160:164	arg1	skin					171:174	dried toad skin	160:174	dried toad skin	160:174	BACKGROUND Cinobufacini, the sterilized hot water extraction of dried toad skin, has been widely used in the treatment of inflammation and cancers.
31568499	7	65	dep	indicated	859:867	arg1	RESULTS					842:848	RESULTS	842:848	RESULTS	842:848	RESULTS Our data indicated that cinobufacini reversed DSS-induced gut dysbiosis and enhanced intestinal barrier integrity.
31568499	1	66	theme	cancers	235:241	arg1	treatment					205:213	the treatment	201:213	the treatment of inflammation and cancers	201:241	BACKGROUND Cinobufacini, the sterilized hot water extraction of dried toad skin, has been widely used in the treatment of inflammation and cancers.
30168792	2	0	theme	strain	103:108	arg1	actinomycete					89:100	A novel actinomycete	81:100	A novel actinomycete	81:100	A novel actinomycete, strain PLAI 1-1T, which formed spiny single spore directly on substrate mycelium was isolated from root tissue of Zingiber montanum.
30168792	2	0	theme	strain	103:108	arg1	1-1T					115:118	strain PLAI 1-1T	103:118	strain PLAI 1-1T	103:118	A novel actinomycete, strain PLAI 1-1T, which formed spiny single spore directly on substrate mycelium was isolated from root tissue of Zingiber montanum.
30168792	9	1	theme	DNA	799:801	arg1	%					815:815	70.6 mol%	807:815	70.6 mol%	807:815	The G+C content of the genomic DNA was 70.6 mol%.
30168792	9	1	theme	DNA	799:801	arg1	G+C content					772:782	The G+C content	768:782	The G+C content of the genomic DNA	768:801	The G+C content of the genomic DNA was 70.6 mol%.
30168792	11	2	theme	DNA-DNA	1103:1109	arg1	hybridization					1111:1123	DNA-DNA hybridization	1103:1123	DNA-DNA hybridization	1103:1123	Based on the data of physiological and biochemical tests, including the result of DNA-DNA hybridization, strain PLAI 1-1T represents a novel species of the genus Jishengella, for which the name Jishengellazingiberis sp.
30168792	10	3	theme	sequence	953:960	arg1	similarity					962:971	the highest 16S rRNA gene sequence similarity	927:971	the highest 16S rRNA gene sequence similarity to Jishengella endophytica DSM 45430T (99.2 %)	927:1018	16S rRNA gene sequence analysis revealed that strain PLAI 1-1T was a member of the genus Jishengella and had the highest 16S rRNA gene sequence similarity to Jishengella endophytica DSM 45430T (99.2 %).
30168792	5	4	theme	whole-cell	410:419	arg1	sugars					421:426	The whole-cell sugars	406:426	The whole-cell sugars of strain PLAI 1-1T	406:446	The whole-cell sugars of strain PLAI 1-1T were glucose, arabinose, xylose, ribose and a trace amount of mannose.
30168792	5	4	theme	whole-cell	410:419	arg1	glucose					453:459	glucose	453:459	glucose	453:459	The whole-cell sugars of strain PLAI 1-1T were glucose, arabinose, xylose, ribose and a trace amount of mannose.
30168792	11	5	theme	genus	1177:1181	arg1	Jishengella					1183:1193	the genus Jishengella	1173:1193	the genus Jishengella	1173:1193	Based on the data of physiological and biochemical tests, including the result of DNA-DNA hybridization, strain PLAI 1-1T represents a novel species of the genus Jishengella, for which the name Jishengellazingiberis sp.
30168792	10	6	theme	Jishengella	976:986	arg1	DSM					1000:1002	Jishengella endophytica DSM 45430T	976:1009	Jishengella endophytica DSM 45430T (99.2 %)	976:1018	16S rRNA gene sequence analysis revealed that strain PLAI 1-1T was a member of the genus Jishengella and had the highest 16S rRNA gene sequence similarity to Jishengella endophytica DSM 45430T (99.2 %).
30168792	10	6	theme	Jishengella	976:986	arg1	%					1017:1017	99.2 %	1012:1017	99.2 %	1012:1017	16S rRNA gene sequence analysis revealed that strain PLAI 1-1T was a member of the genus Jishengella and had the highest 16S rRNA gene sequence similarity to Jishengella endophytica DSM 45430T (99.2 %).
30168792	11	7	theme	Jishengellazingiberis	1215:1235	arg1	sp					1237:1238	the name Jishengellazingiberis sp	1206:1238	the name Jishengellazingiberis sp	1206:1238	Based on the data of physiological and biochemical tests, including the result of DNA-DNA hybridization, strain PLAI 1-1T represents a novel species of the genus Jishengella, for which the name Jishengellazingiberis sp.
30168792	7	8	theme	major	672:676	arg1	MK-9					694:697	MK-9	694:697	MK-9 (H4)	694:702	The major menaquinone was MK-9 (H4).
30168792	7	8	theme	major	672:676	arg1	menaquinone					678:688	The major menaquinone	668:688	The major menaquinone	668:688	The major menaquinone was MK-9 (H4).
30168792	9	9	theme	70.6 mol	807:814	arg1	%					815:815	70.6 mol%	807:815	70.6 mol%	807:815	The G+C content of the genomic DNA was 70.6 mol%.
30168792	9	9	theme	70.6 mol	807:814	arg1	G+C content					772:782	The G+C content	768:782	The G+C content of the genomic DNA	768:801	The G+C content of the genomic DNA was 70.6 mol%.
30168792	4	10	theme	acid	387:390	arg1	glycolyl					396:403	glycolyl	396:403	glycolyl	396:403	The acyl type of the cell-wall muramic acid was glycolyl.
30168792	4	10	theme	acid	387:390	arg1	type					357:360	The acyl type	348:360	The acyl type of the cell-wall muramic acid	348:390	The acyl type of the cell-wall muramic acid was glycolyl.
30168792	1	11	theme	root	47:50	arg1	tissue					52:57	root tissue	47:57	root tissue of Zingiber montanum	47:78	nov., isolated from root tissue of Zingiber montanum.
30168792	10	12	theme	genus	901:905	arg1	Jishengella					907:917	the genus Jishengella	897:917	the genus Jishengella	897:917	16S rRNA gene sequence analysis revealed that strain PLAI 1-1T was a member of the genus Jishengella and had the highest 16S rRNA gene sequence similarity to Jishengella endophytica DSM 45430T (99.2 %).
30168792	2	13	attach	isolated	188:195	arg2	actinomycete					89:100	A novel actinomycete	81:100	A novel actinomycete	81:100	A novel actinomycete, strain PLAI 1-1T, which formed spiny single spore directly on substrate mycelium was isolated from root tissue of Zingiber montanum.
30168792	2	13	attach	isolated	188:195	arg1	tissue					207:212	root tissue	202:212	root tissue of Zingiber montanum	202:233	A novel actinomycete, strain PLAI 1-1T, which formed spiny single spore directly on substrate mycelium was isolated from root tissue of Zingiber montanum.
30168792	2	13	attach	isolated	188:195	arg2	1-1T					115:118	strain PLAI 1-1T	103:118	strain PLAI 1-1T	103:118	A novel actinomycete, strain PLAI 1-1T, which formed spiny single spore directly on substrate mycelium was isolated from root tissue of Zingiber montanum.
30168792	11	14	theme	PLAI	1133:1136	arg1	1-1T					1138:1141	strain PLAI 1-1T	1126:1141	strain PLAI 1-1T	1126:1141	Based on the data of physiological and biochemical tests, including the result of DNA-DNA hybridization, strain PLAI 1-1T represents a novel species of the genus Jishengella, for which the name Jishengellazingiberis sp.
30168792	11	15	theme	biochemical	1060:1070	arg1	result					1093:1098	the result	1089:1098	the result of DNA-DNA hybridization	1089:1123	Based on the data of physiological and biochemical tests, including the result of DNA-DNA hybridization, strain PLAI 1-1T represents a novel species of the genus Jishengella, for which the name Jishengellazingiberis sp.
30168792	11	15	theme	biochemical	1060:1070	arg1	tests					1072:1076	physiological and biochemical tests	1042:1076	physiological and biochemical tests	1042:1076	Based on the data of physiological and biochemical tests, including the result of DNA-DNA hybridization, strain PLAI 1-1T represents a novel species of the genus Jishengella, for which the name Jishengellazingiberis sp.
30168792	10	16	theme	PLAI	871:874	arg1	1-1T					876:879	strain PLAI 1-1T	864:879	strain PLAI 1-1T	864:879	16S rRNA gene sequence analysis revealed that strain PLAI 1-1T was a member of the genus Jishengella and had the highest 16S rRNA gene sequence similarity to Jishengella endophytica DSM 45430T (99.2 %).
30168792	10	16	theme	PLAI	871:874	arg1	member					887:892	a member	885:892	a member of the genus Jishengella	885:917	16S rRNA gene sequence analysis revealed that strain PLAI 1-1T was a member of the genus Jishengella and had the highest 16S rRNA gene sequence similarity to Jishengella endophytica DSM 45430T (99.2 %).
30168792	11	17	theme	hybridization	1111:1123	arg1	result					1093:1098	the result	1089:1098	the result of DNA-DNA hybridization	1089:1123	Based on the data of physiological and biochemical tests, including the result of DNA-DNA hybridization, strain PLAI 1-1T represents a novel species of the genus Jishengella, for which the name Jishengellazingiberis sp.
30168792	10	18	contain	had	923:925	arg2	similarity					962:971	the highest 16S rRNA gene sequence similarity	927:971	the highest 16S rRNA gene sequence similarity to Jishengella endophytica DSM 45430T (99.2 %)	927:1018	16S rRNA gene sequence analysis revealed that strain PLAI 1-1T was a member of the genus Jishengella and had the highest 16S rRNA gene sequence similarity to Jishengella endophytica DSM 45430T (99.2 %).
30168792	10	18	contain	had	923:925	arg1	1-1T					876:879	strain PLAI 1-1T	864:879	strain PLAI 1-1T	864:879	16S rRNA gene sequence analysis revealed that strain PLAI 1-1T was a member of the genus Jishengella and had the highest 16S rRNA gene sequence similarity to Jishengella endophytica DSM 45430T (99.2 %).
30168792	10	18	contain	had	923:925	arg1	member					887:892	a member	885:892	a member of the genus Jishengella	885:917	16S rRNA gene sequence analysis revealed that strain PLAI 1-1T was a member of the genus Jishengella and had the highest 16S rRNA gene sequence similarity to Jishengella endophytica DSM 45430T (99.2 %).
30168792	8	19	theme	cellular	714:721	arg1	iso-C15 					740:747	iso-C15 	740:747	iso-C15 	740:747	The main cellular fatty acids were iso-C15 : 0 and C17 : 1ω8c.
30168792	8	19	theme	cellular	714:721	arg1	acids					729:733	The main cellular fatty acids	705:733	The main cellular fatty acids	705:733	The main cellular fatty acids were iso-C15 : 0 and C17 : 1ω8c.
30168792	1	20	theme	Zingiber	62:69	arg1	montanum					71:78	Zingiber montanum	62:78	Zingiber montanum	62:78	nov., isolated from root tissue of Zingiber montanum.
30168792	0	21	theme	zingiberis	12:21	arg1	sp					23:24	Jishengella zingiberis sp	0:24	Jishengella zingiberis sp.	0:25	Jishengella zingiberis sp.
30168792	8	22	theme	main	709:712	arg1	iso-C15 					740:747	iso-C15 	740:747	iso-C15 	740:747	The main cellular fatty acids were iso-C15 : 0 and C17 : 1ω8c.
30168792	8	22	theme	main	709:712	arg1	acids					729:733	The main cellular fatty acids	705:733	The main cellular fatty acids	705:733	The main cellular fatty acids were iso-C15 : 0 and C17 : 1ω8c.
30168792	6	23	theme	membrane	523:530	arg1	diphosphatidylglycerol					551:572	diphosphatidylglycerol	551:572	diphosphatidylglycerol	551:572	The membrane phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylserine and phosphatidylinositol.
30168792	6	23	theme	membrane	523:530	arg1	phospholipids					532:544	The membrane phospholipids	519:544	The membrane phospholipids	519:544	The membrane phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylserine and phosphatidylinositol.
30168792	1	24	theme	montanum	71:78	arg1	tissue					52:57	root tissue	47:57	root tissue of Zingiber montanum	47:78	nov., isolated from root tissue of Zingiber montanum.
30168792	0	25	theme	Jishengella	0:10	arg1	sp					23:24	Jishengella zingiberis sp	0:24	Jishengella zingiberis sp.	0:25	Jishengella zingiberis sp.
30168792	11	26	theme	physiological	1042:1054	arg1	result					1093:1098	the result	1089:1098	the result of DNA-DNA hybridization	1089:1123	Based on the data of physiological and biochemical tests, including the result of DNA-DNA hybridization, strain PLAI 1-1T represents a novel species of the genus Jishengella, for which the name Jishengellazingiberis sp.
30168792	11	26	theme	physiological	1042:1054	arg1	tests					1072:1076	physiological and biochemical tests	1042:1076	physiological and biochemical tests	1042:1076	Based on the data of physiological and biochemical tests, including the result of DNA-DNA hybridization, strain PLAI 1-1T represents a novel species of the genus Jishengella, for which the name Jishengellazingiberis sp.
30168792	4	27	theme	muramic	379:385	arg1	acid					387:390	the cell-wall muramic acid	365:390	the cell-wall muramic acid	365:390	The acyl type of the cell-wall muramic acid was glycolyl.
30168792	11	28	theme	novel	1156:1160	arg1	species					1162:1168	a novel species	1154:1168	a novel species	1154:1168	Based on the data of physiological and biochemical tests, including the result of DNA-DNA hybridization, strain PLAI 1-1T represents a novel species of the genus Jishengella, for which the name Jishengellazingiberis sp.
30168792	8	29	theme	fatty	723:727	arg1	iso-C15 					740:747	iso-C15 	740:747	iso-C15 	740:747	The main cellular fatty acids were iso-C15 : 0 and C17 : 1ω8c.
30168792	8	29	theme	fatty	723:727	arg1	acids					729:733	The main cellular fatty acids	705:733	The main cellular fatty acids	705:733	The main cellular fatty acids were iso-C15 : 0 and C17 : 1ω8c.
30168792	4	30	theme	cell-wall	369:377	arg1	acid					387:390	the cell-wall muramic acid	365:390	the cell-wall muramic acid	365:390	The acyl type of the cell-wall muramic acid was glycolyl.
30168792	10	31	theme	Jishengella	907:917	arg1	1-1T					876:879	strain PLAI 1-1T	864:879	strain PLAI 1-1T	864:879	16S rRNA gene sequence analysis revealed that strain PLAI 1-1T was a member of the genus Jishengella and had the highest 16S rRNA gene sequence similarity to Jishengella endophytica DSM 45430T (99.2 %).
30168792	10	31	theme	Jishengella	907:917	arg1	member					887:892	a member	885:892	a member of the genus Jishengella	885:917	16S rRNA gene sequence analysis revealed that strain PLAI 1-1T was a member of the genus Jishengella and had the highest 16S rRNA gene sequence similarity to Jishengella endophytica DSM 45430T (99.2 %).
30168792	10	32	theme	gene	827:830	arg1	analysis					841:848	16S rRNA gene sequence analysis	818:848	16S rRNA gene sequence analysis	818:848	16S rRNA gene sequence analysis revealed that strain PLAI 1-1T was a member of the genus Jishengella and had the highest 16S rRNA gene sequence similarity to Jishengella endophytica DSM 45430T (99.2 %).
30168792	3	33	theme	meso-diaminopimelic	258:276	arg1	acid					278:281	meso-diaminopimelic acid	258:281	meso-diaminopimelic acid	258:281	The isolate contained meso-diaminopimelic acid and 3-hydroxydiaminopimelic acid in the cell-wall peptidoglycan.
30168792	10	34	theme	strain	864:869	arg1	1-1T					876:879	strain PLAI 1-1T	864:879	strain PLAI 1-1T	864:879	16S rRNA gene sequence analysis revealed that strain PLAI 1-1T was a member of the genus Jishengella and had the highest 16S rRNA gene sequence similarity to Jishengella endophytica DSM 45430T (99.2 %).
30168792	10	34	theme	strain	864:869	arg1	member					887:892	a member	885:892	a member of the genus Jishengella	885:917	16S rRNA gene sequence analysis revealed that strain PLAI 1-1T was a member of the genus Jishengella and had the highest 16S rRNA gene sequence similarity to Jishengella endophytica DSM 45430T (99.2 %).
30168792	11	35	theme	name	1210:1213	arg1	sp					1237:1238	the name Jishengellazingiberis sp	1206:1238	the name Jishengellazingiberis sp	1206:1238	Based on the data of physiological and biochemical tests, including the result of DNA-DNA hybridization, strain PLAI 1-1T represents a novel species of the genus Jishengella, for which the name Jishengellazingiberis sp.
30168792	10	36	theme	rRNA	822:825	arg1	analysis					841:848	16S rRNA gene sequence analysis	818:848	16S rRNA gene sequence analysis	818:848	16S rRNA gene sequence analysis revealed that strain PLAI 1-1T was a member of the genus Jishengella and had the highest 16S rRNA gene sequence similarity to Jishengella endophytica DSM 45430T (99.2 %).
30168792	10	37	theme	endophytica	988:998	arg1	DSM					1000:1002	Jishengella endophytica DSM 45430T	976:1009	Jishengella endophytica DSM 45430T (99.2 %)	976:1018	16S rRNA gene sequence analysis revealed that strain PLAI 1-1T was a member of the genus Jishengella and had the highest 16S rRNA gene sequence similarity to Jishengella endophytica DSM 45430T (99.2 %).
30168792	10	37	theme	endophytica	988:998	arg1	%					1017:1017	99.2 %	1012:1017	99.2 %	1012:1017	16S rRNA gene sequence analysis revealed that strain PLAI 1-1T was a member of the genus Jishengella and had the highest 16S rRNA gene sequence similarity to Jishengella endophytica DSM 45430T (99.2 %).
30168792	10	38	theme	rRNA	943:946	arg1	similarity					962:971	the highest 16S rRNA gene sequence similarity	927:971	the highest 16S rRNA gene sequence similarity to Jishengella endophytica DSM 45430T (99.2 %)	927:1018	16S rRNA gene sequence analysis revealed that strain PLAI 1-1T was a member of the genus Jishengella and had the highest 16S rRNA gene sequence similarity to Jishengella endophytica DSM 45430T (99.2 %).
30168792	3	39	contain	contained	248:256	arg2	acid					311:314	3-hydroxydiaminopimelic acid	287:314	3-hydroxydiaminopimelic acid	287:314	The isolate contained meso-diaminopimelic acid and 3-hydroxydiaminopimelic acid in the cell-wall peptidoglycan.
30168792	3	39	contain	contained	248:256	arg1	peptidoglycan					333:345	the cell-wall peptidoglycan	319:345	the cell-wall peptidoglycan	319:345	The isolate contained meso-diaminopimelic acid and 3-hydroxydiaminopimelic acid in the cell-wall peptidoglycan.
30168792	3	39	contain	contained	248:256	arg1	isolate					240:246	The isolate	236:246	The isolate	236:246	The isolate contained meso-diaminopimelic acid and 3-hydroxydiaminopimelic acid in the cell-wall peptidoglycan.
30168792	3	39	contain	contained	248:256	arg2	isolate					240:246	The isolate	236:246	The isolate	236:246	The isolate contained meso-diaminopimelic acid and 3-hydroxydiaminopimelic acid in the cell-wall peptidoglycan.
30168792	3	39	contain	contained	248:256	arg2	acid					278:281	meso-diaminopimelic acid	258:281	meso-diaminopimelic acid	258:281	The isolate contained meso-diaminopimelic acid and 3-hydroxydiaminopimelic acid in the cell-wall peptidoglycan.
30168792	10	40	theme	16S	818:820	arg1	rRNA					822:825	16S rRNA	818:825	16S rRNA gene sequence analysis	818:848	16S rRNA gene sequence analysis revealed that strain PLAI 1-1T was a member of the genus Jishengella and had the highest 16S rRNA gene sequence similarity to Jishengella endophytica DSM 45430T (99.2 %).
30168792	2	41	theme	novel	83:87	arg1	actinomycete					89:100	A novel actinomycete	81:100	A novel actinomycete	81:100	A novel actinomycete, strain PLAI 1-1T, which formed spiny single spore directly on substrate mycelium was isolated from root tissue of Zingiber montanum.
30168792	2	41	theme	novel	83:87	arg1	1-1T					115:118	strain PLAI 1-1T	103:118	strain PLAI 1-1T	103:118	A novel actinomycete, strain PLAI 1-1T, which formed spiny single spore directly on substrate mycelium was isolated from root tissue of Zingiber montanum.
30168792	2	42	theme	single	140:145	arg1	spore					147:151	spiny single spore	134:151	spiny single spore	134:151	A novel actinomycete, strain PLAI 1-1T, which formed spiny single spore directly on substrate mycelium was isolated from root tissue of Zingiber montanum.
30168792	2	43	theme	root	202:205	arg1	tissue					207:212	root tissue	202:212	root tissue of Zingiber montanum	202:233	A novel actinomycete, strain PLAI 1-1T, which formed spiny single spore directly on substrate mycelium was isolated from root tissue of Zingiber montanum.
30168792	5	44	theme	mannose	510:516	arg1	ribose					481:486	ribose	481:486	ribose	481:486	The whole-cell sugars of strain PLAI 1-1T were glucose, arabinose, xylose, ribose and a trace amount of mannose.
30168792	5	44	theme	mannose	510:516	arg1	mannose					510:516	mannose	510:516	mannose	510:516	The whole-cell sugars of strain PLAI 1-1T were glucose, arabinose, xylose, ribose and a trace amount of mannose.
30168792	5	44	theme	mannose	510:516	arg1	sugars					421:426	The whole-cell sugars	406:426	The whole-cell sugars of strain PLAI 1-1T	406:446	The whole-cell sugars of strain PLAI 1-1T were glucose, arabinose, xylose, ribose and a trace amount of mannose.
30168792	5	44	theme	mannose	510:516	arg1	glucose					453:459	glucose	453:459	glucose	453:459	The whole-cell sugars of strain PLAI 1-1T were glucose, arabinose, xylose, ribose and a trace amount of mannose.
30168792	5	44	theme	mannose	510:516	arg1	arabinose					462:470	arabinose	462:470	arabinose	462:470	The whole-cell sugars of strain PLAI 1-1T were glucose, arabinose, xylose, ribose and a trace amount of mannose.
30168792	5	44	theme	mannose	510:516	arg1	amount					500:505	a trace amount	492:505	a trace amount of mannose	492:516	The whole-cell sugars of strain PLAI 1-1T were glucose, arabinose, xylose, ribose and a trace amount of mannose.
30168792	5	44	theme	mannose	510:516	arg1	xylose					473:478	xylose	473:478	xylose	473:478	The whole-cell sugars of strain PLAI 1-1T were glucose, arabinose, xylose, ribose and a trace amount of mannose.
30168792	13	45	theme	=TBRC	1289:1293	arg1	113144T					1306:1312	=TBRC 7644T=NBRC 113144T	1289:1312	=TBRC 7644T=NBRC 113144T	1289:1312	The type strain is PLAI 1-1T (=TBRC 7644T=NBRC 113144T).
30168792	13	45	theme	=TBRC	1289:1293	arg1	1-1T					1283:1286	PLAI 1-1T	1278:1286	PLAI 1-1T (=TBRC 7644T=NBRC 113144T)	1278:1313	The type strain is PLAI 1-1T (=TBRC 7644T=NBRC 113144T).
30168792	3	46	theme	3-hydroxydiaminopimelic	287:309	arg1	acid					311:314	3-hydroxydiaminopimelic acid	287:314	3-hydroxydiaminopimelic acid	287:314	The isolate contained meso-diaminopimelic acid and 3-hydroxydiaminopimelic acid in the cell-wall peptidoglycan.
30168792	2	47	theme	spiny	134:138	arg1	spore					147:151	spiny single spore	134:151	spiny single spore	134:151	A novel actinomycete, strain PLAI 1-1T, which formed spiny single spore directly on substrate mycelium was isolated from root tissue of Zingiber montanum.
30168792	10	48	theme	highest	931:937	arg1	similarity					962:971	the highest 16S rRNA gene sequence similarity	927:971	the highest 16S rRNA gene sequence similarity to Jishengella endophytica DSM 45430T (99.2 %)	927:1018	16S rRNA gene sequence analysis revealed that strain PLAI 1-1T was a member of the genus Jishengella and had the highest 16S rRNA gene sequence similarity to Jishengella endophytica DSM 45430T (99.2 %).
30168792	8	49	dep	iso-C15 	740:747	arg1	 0					749:750	 0	749:750	 0	749:750	The main cellular fatty acids were iso-C15 : 0 and C17 : 1ω8c.
30168792	8	49	dep	iso-C15 	740:747	arg1	C17 					756:759	C17 	756:759	C17 	756:759	The main cellular fatty acids were iso-C15 : 0 and C17 : 1ω8c.
30168792	11	50	theme	tests	1072:1076	arg1	data					1034:1037	the data	1030:1037	the data of physiological and biochemical tests, including the result of DNA-DNA hybridization	1030:1123	Based on the data of physiological and biochemical tests, including the result of DNA-DNA hybridization, strain PLAI 1-1T represents a novel species of the genus Jishengella, for which the name Jishengellazingiberis sp.
30168792	8	51	dep	 0	749:750	arg1	 1ω8c					761:765	 1ω8c	761:765	iso-C15 : 0 and C17 : 1ω8c	740:765	The main cellular fatty acids were iso-C15 : 0 and C17 : 1ω8c.
30168792	5	52	theme	strain	431:436	arg1	1-1T					443:446	strain PLAI 1-1T	431:446	strain PLAI 1-1T	431:446	The whole-cell sugars of strain PLAI 1-1T were glucose, arabinose, xylose, ribose and a trace amount of mannose.
30168792	10	53	theme	gene	948:951	arg1	similarity					962:971	the highest 16S rRNA gene sequence similarity	927:971	the highest 16S rRNA gene sequence similarity to Jishengella endophytica DSM 45430T (99.2 %)	927:1018	16S rRNA gene sequence analysis revealed that strain PLAI 1-1T was a member of the genus Jishengella and had the highest 16S rRNA gene sequence similarity to Jishengella endophytica DSM 45430T (99.2 %).
30168792	11	54	theme	Jishengella	1183:1193	arg1	species					1162:1168	a novel species	1154:1168	a novel species	1154:1168	Based on the data of physiological and biochemical tests, including the result of DNA-DNA hybridization, strain PLAI 1-1T represents a novel species of the genus Jishengella, for which the name Jishengellazingiberis sp.
30168792	4	55	theme	acyl	352:355	arg1	glycolyl					396:403	glycolyl	396:403	glycolyl	396:403	The acyl type of the cell-wall muramic acid was glycolyl.
30168792	4	55	theme	acyl	352:355	arg1	type					357:360	The acyl type	348:360	The acyl type of the cell-wall muramic acid	348:390	The acyl type of the cell-wall muramic acid was glycolyl.
30168792	5	56	theme	PLAI	438:441	arg1	1-1T					443:446	strain PLAI 1-1T	431:446	strain PLAI 1-1T	431:446	The whole-cell sugars of strain PLAI 1-1T were glucose, arabinose, xylose, ribose and a trace amount of mannose.
30168792	10	57	theme	16S	939:941	arg1	similarity					962:971	the highest 16S rRNA gene sequence similarity	927:971	the highest 16S rRNA gene sequence similarity to Jishengella endophytica DSM 45430T (99.2 %)	927:1018	16S rRNA gene sequence analysis revealed that strain PLAI 1-1T was a member of the genus Jishengella and had the highest 16S rRNA gene sequence similarity to Jishengella endophytica DSM 45430T (99.2 %).
30168792	2	58	theme	substrate	165:173	arg1	mycelium					175:182	substrate mycelium	165:182	substrate mycelium	165:182	A novel actinomycete, strain PLAI 1-1T, which formed spiny single spore directly on substrate mycelium was isolated from root tissue of Zingiber montanum.
30168792	2	59	theme	montanum	226:233	arg1	tissue					207:212	root tissue	202:212	root tissue of Zingiber montanum	202:233	A novel actinomycete, strain PLAI 1-1T, which formed spiny single spore directly on substrate mycelium was isolated from root tissue of Zingiber montanum.
30168792	11	60	theme	strain	1126:1131	arg1	1-1T					1138:1141	strain PLAI 1-1T	1126:1141	strain PLAI 1-1T	1126:1141	Based on the data of physiological and biochemical tests, including the result of DNA-DNA hybridization, strain PLAI 1-1T represents a novel species of the genus Jishengella, for which the name Jishengellazingiberis sp.
30168792	10	61	theme	sequence	832:839	arg1	analysis					841:848	16S rRNA gene sequence analysis	818:848	16S rRNA gene sequence analysis	818:848	16S rRNA gene sequence analysis revealed that strain PLAI 1-1T was a member of the genus Jishengella and had the highest 16S rRNA gene sequence similarity to Jishengella endophytica DSM 45430T (99.2 %).
30168792	5	62	theme	1-1T	443:446	arg1	sugars					421:426	The whole-cell sugars	406:426	The whole-cell sugars of strain PLAI 1-1T	406:446	The whole-cell sugars of strain PLAI 1-1T were glucose, arabinose, xylose, ribose and a trace amount of mannose.
30168792	5	62	theme	1-1T	443:446	arg1	glucose					453:459	glucose	453:459	glucose	453:459	The whole-cell sugars of strain PLAI 1-1T were glucose, arabinose, xylose, ribose and a trace amount of mannose.
30168792	13	63	theme	PLAI	1278:1281	arg1	113144T					1306:1312	=TBRC 7644T=NBRC 113144T	1289:1312	=TBRC 7644T=NBRC 113144T	1289:1312	The type strain is PLAI 1-1T (=TBRC 7644T=NBRC 113144T).
30168792	13	63	theme	PLAI	1278:1281	arg1	strain					1268:1273	The type strain	1259:1273	The type strain	1259:1273	The type strain is PLAI 1-1T (=TBRC 7644T=NBRC 113144T).
30168792	13	63	theme	PLAI	1278:1281	arg1	1-1T					1283:1286	PLAI 1-1T	1278:1286	PLAI 1-1T (=TBRC 7644T=NBRC 113144T)	1278:1313	The type strain is PLAI 1-1T (=TBRC 7644T=NBRC 113144T).
30168792	3	64	theme	cell-wall	323:331	arg1	peptidoglycan					333:345	the cell-wall peptidoglycan	319:345	the cell-wall peptidoglycan	319:345	The isolate contained meso-diaminopimelic acid and 3-hydroxydiaminopimelic acid in the cell-wall peptidoglycan.
30168792	2	65	theme	Zingiber	217:224	arg1	montanum					226:233	Zingiber montanum	217:233	Zingiber montanum	217:233	A novel actinomycete, strain PLAI 1-1T, which formed spiny single spore directly on substrate mycelium was isolated from root tissue of Zingiber montanum.
30168792	5	66	theme	trace	494:498	arg1	amount					500:505	a trace amount	492:505	a trace amount of mannose	492:516	The whole-cell sugars of strain PLAI 1-1T were glucose, arabinose, xylose, ribose and a trace amount of mannose.
30168792	5	66	theme	trace	494:498	arg1	mannose					510:516	mannose	510:516	mannose	510:516	The whole-cell sugars of strain PLAI 1-1T were glucose, arabinose, xylose, ribose and a trace amount of mannose.
30168792	13	67	theme	type	1263:1266	arg1	strain					1268:1273	The type strain	1259:1273	The type strain	1259:1273	The type strain is PLAI 1-1T (=TBRC 7644T=NBRC 113144T).
30168792	13	67	theme	type	1263:1266	arg1	1-1T					1283:1286	PLAI 1-1T	1278:1286	PLAI 1-1T (=TBRC 7644T=NBRC 113144T)	1278:1313	The type strain is PLAI 1-1T (=TBRC 7644T=NBRC 113144T).
30168792	2	68	theme	PLAI	110:113	arg1	actinomycete					89:100	A novel actinomycete	81:100	A novel actinomycete	81:100	A novel actinomycete, strain PLAI 1-1T, which formed spiny single spore directly on substrate mycelium was isolated from root tissue of Zingiber montanum.
30168792	2	68	theme	PLAI	110:113	arg1	1-1T					115:118	strain PLAI 1-1T	103:118	strain PLAI 1-1T	103:118	A novel actinomycete, strain PLAI 1-1T, which formed spiny single spore directly on substrate mycelium was isolated from root tissue of Zingiber montanum.
30168792	9	69	theme	genomic	791:797	arg1	DNA					799:801	the genomic DNA	787:801	the genomic DNA	787:801	The G+C content of the genomic DNA was 70.6 mol%.
30168792	13	70	theme	7644T=NBRC	1295:1304	arg1	113144T					1306:1312	=TBRC 7644T=NBRC 113144T	1289:1312	=TBRC 7644T=NBRC 113144T	1289:1312	The type strain is PLAI 1-1T (=TBRC 7644T=NBRC 113144T).
30168792	13	70	theme	7644T=NBRC	1295:1304	arg1	1-1T					1283:1286	PLAI 1-1T	1278:1286	PLAI 1-1T (=TBRC 7644T=NBRC 113144T)	1278:1313	The type strain is PLAI 1-1T (=TBRC 7644T=NBRC 113144T).
29595091	1	0	theme	products	170:177	arg1	composition					148:158	the bioactive composition	134:158	the bioactive composition of tempeh products	134:177	Objectives: This study evaluated the bioactive composition of tempeh products and examined the effects of tempeh on BV-2 microglial cell cytotoxicity, neurotrophic effects, and expression of inflammatory genes.Methods: Tempeh products included soybean fermented by Rhizopus, soybean fermented through cocultivation with Rhizopus and Lactobacillus, and red bean fermented through cocultivation with Rhizopus and Lactobacillus (RT-C).
29595091	3	1	theme	water	826:830	arg1	extracts					832:839	The tempeh water extracts	815:839	The tempeh water extracts	815:839	The tempeh water extracts, especially RT-C, reduced the formation of LPS-induced reactive oxygen species, downregulated the levels of nitric oxide synthase and phospho-cyclic-AMP response element-binding protein, and upregulated the expression of brain-derived neurotrophic factor (BDNF).
29595091	3	1	theme	water	826:830	arg1	RT-C					853:856	RT-C	853:856	especially RT-C	842:856	The tempeh water extracts, especially RT-C, reduced the formation of LPS-induced reactive oxygen species, downregulated the levels of nitric oxide synthase and phospho-cyclic-AMP response element-binding protein, and upregulated the expression of brain-derived neurotrophic factor (BDNF).
29595091	2	2	contain	had	737:739	arg1	extract					729:735	RT-C water extract	718:735	RT-C water extract	718:735	We analyzed the bioactive contents of tempeh extracts and evaluated the effects of tempeh water extract on lipopolysaccharide (LPS)-treated BV-2 cells.Results: The results showed that RT-C water extract had the highest concentrations of γ-aminobutyric acid (GABA) and anthocyanin.
29595091	2	2	contain	had	737:739	arg2	concentrations					753:766	the highest concentrations	741:766	the highest concentrations of γ-aminobutyric acid (GABA) and anthocyanin	741:812	We analyzed the bioactive contents of tempeh extracts and evaluated the effects of tempeh water extract on lipopolysaccharide (LPS)-treated BV-2 cells.Results: The results showed that RT-C water extract had the highest concentrations of γ-aminobutyric acid (GABA) and anthocyanin.
29595091	2	3	theme	extract	630:636	arg1	effects					606:612	the effects	602:612	the effects of tempeh water extract on lipopolysaccharide (LPS)-treated BV-2 cells.Results	602:691	We analyzed the bioactive contents of tempeh extracts and evaluated the effects of tempeh water extract on lipopolysaccharide (LPS)-treated BV-2 cells.Results: The results showed that RT-C water extract had the highest concentrations of γ-aminobutyric acid (GABA) and anthocyanin.
29595091	3	4	theme	LPS-induced	884:894	arg1	species					912:918	LPS-induced reactive oxygen species	884:918	LPS-induced reactive oxygen species	884:918	The tempeh water extracts, especially RT-C, reduced the formation of LPS-induced reactive oxygen species, downregulated the levels of nitric oxide synthase and phospho-cyclic-AMP response element-binding protein, and upregulated the expression of brain-derived neurotrophic factor (BDNF).
29595091	3	5	theme	species	912:918	arg1	formation					871:879	the formation	867:879	the formation of LPS-induced reactive oxygen species	867:918	The tempeh water extracts, especially RT-C, reduced the formation of LPS-induced reactive oxygen species, downregulated the levels of nitric oxide synthase and phospho-cyclic-AMP response element-binding protein, and upregulated the expression of brain-derived neurotrophic factor (BDNF).
29595091	2	6	theme	water	624:628	arg1	extract					630:636	tempeh water extract	617:636	tempeh water extract	617:636	We analyzed the bioactive contents of tempeh extracts and evaluated the effects of tempeh water extract on lipopolysaccharide (LPS)-treated BV-2 cells.Results: The results showed that RT-C water extract had the highest concentrations of γ-aminobutyric acid (GABA) and anthocyanin.
29595091	3	7	theme	brain-derived	1062:1074	arg1	factor					1089:1094	brain-derived neurotrophic factor	1062:1094	brain-derived neurotrophic factor (BDNF)	1062:1101	The tempeh water extracts, especially RT-C, reduced the formation of LPS-induced reactive oxygen species, downregulated the levels of nitric oxide synthase and phospho-cyclic-AMP response element-binding protein, and upregulated the expression of brain-derived neurotrophic factor (BDNF).
29595091	3	7	theme	brain-derived	1062:1074	arg1	BDNF					1097:1100	BDNF	1097:1100	BDNF	1097:1100	The tempeh water extracts, especially RT-C, reduced the formation of LPS-induced reactive oxygen species, downregulated the levels of nitric oxide synthase and phospho-cyclic-AMP response element-binding protein, and upregulated the expression of brain-derived neurotrophic factor (BDNF).
29595091	4	8	theme	BDNF	1294:1297	arg1	expression					1280:1289	the expression	1276:1289	the expression of BDNF in LPS-induced BV-2 cells	1276:1323	Discussion: Our data demonstrate that RT-C has the highest concentrations of GABA and anthocyanin, more effectively reduces oxidative stress and inflammation, and increases the expression of BDNF in LPS-induced BV-2 cells.
29595091	3	9	theme	reactive	896:903	arg1	species					912:918	LPS-induced reactive oxygen species	884:918	LPS-induced reactive oxygen species	884:918	The tempeh water extracts, especially RT-C, reduced the formation of LPS-induced reactive oxygen species, downregulated the levels of nitric oxide synthase and phospho-cyclic-AMP response element-binding protein, and upregulated the expression of brain-derived neurotrophic factor (BDNF).
29595091	2	10	theme	bioactive	550:558	arg1	contents					560:567	the bioactive contents	546:567	the bioactive contents of tempeh extracts	546:586	We analyzed the bioactive contents of tempeh extracts and evaluated the effects of tempeh water extract on lipopolysaccharide (LPS)-treated BV-2 cells.Results: The results showed that RT-C water extract had the highest concentrations of γ-aminobutyric acid (GABA) and anthocyanin.
29595091	2	11	theme	tempeh	617:622	arg1	extract					630:636	tempeh water extract	617:636	tempeh water extract	617:636	We analyzed the bioactive contents of tempeh extracts and evaluated the effects of tempeh water extract on lipopolysaccharide (LPS)-treated BV-2 cells.Results: The results showed that RT-C water extract had the highest concentrations of γ-aminobutyric acid (GABA) and anthocyanin.
29595091	2	12	theme	BV-2	674:677	arg1	cells.Results					679:691	lipopolysaccharide (LPS)-treated BV-2 cells.Results	641:691	lipopolysaccharide (LPS)-treated BV-2 cells.Results	641:691	We analyzed the bioactive contents of tempeh extracts and evaluated the effects of tempeh water extract on lipopolysaccharide (LPS)-treated BV-2 cells.Results: The results showed that RT-C water extract had the highest concentrations of γ-aminobutyric acid (GABA) and anthocyanin.
29595091	2	13	from	effects	606:612	arg1	cells.Results					679:691	lipopolysaccharide (LPS)-treated BV-2 cells.Results	641:691	lipopolysaccharide (LPS)-treated BV-2 cells.Results	641:691	We analyzed the bioactive contents of tempeh extracts and evaluated the effects of tempeh water extract on lipopolysaccharide (LPS)-treated BV-2 cells.Results: The results showed that RT-C water extract had the highest concentrations of γ-aminobutyric acid (GABA) and anthocyanin.
29595091	0	14	from	damage	68:73	arg1	cells					94:98	BV-2 microglial cells	78:98	BV-2 microglial cells	78:98	Neuroprotective effect of tempeh against lipopolysaccharide-induced damage in BV-2 microglial cells.
29595091	2	15	theme	extracts	579:586	arg1	contents					560:567	the bioactive contents	546:567	the bioactive contents of tempeh extracts	546:586	We analyzed the bioactive contents of tempeh extracts and evaluated the effects of tempeh water extract on lipopolysaccharide (LPS)-treated BV-2 cells.Results: The results showed that RT-C water extract had the highest concentrations of γ-aminobutyric acid (GABA) and anthocyanin.
29595091	2	16	theme	-treated	665:672	arg1	cells.Results					679:691	lipopolysaccharide (LPS)-treated BV-2 cells.Results	641:691	lipopolysaccharide (LPS)-treated BV-2 cells.Results	641:691	We analyzed the bioactive contents of tempeh extracts and evaluated the effects of tempeh water extract on lipopolysaccharide (LPS)-treated BV-2 cells.Results: The results showed that RT-C water extract had the highest concentrations of γ-aminobutyric acid (GABA) and anthocyanin.
29595091	4	17	from	expression	1280:1289	arg1	cells					1319:1323	LPS-induced BV-2 cells	1302:1323	LPS-induced BV-2 cells	1302:1323	Discussion: Our data demonstrate that RT-C has the highest concentrations of GABA and anthocyanin, more effectively reduces oxidative stress and inflammation, and increases the expression of BDNF in LPS-induced BV-2 cells.
29595091	3	18	link	brain-derived	1062:1074	arg1	factor					1089:1094	brain-derived neurotrophic factor	1062:1094	brain-derived neurotrophic factor (BDNF)	1062:1101	The tempeh water extracts, especially RT-C, reduced the formation of LPS-induced reactive oxygen species, downregulated the levels of nitric oxide synthase and phospho-cyclic-AMP response element-binding protein, and upregulated the expression of brain-derived neurotrophic factor (BDNF).
29595091	3	18	link	brain-derived	1062:1074	arg1	BDNF					1097:1100	BDNF	1097:1100	BDNF	1097:1100	The tempeh water extracts, especially RT-C, reduced the formation of LPS-induced reactive oxygen species, downregulated the levels of nitric oxide synthase and phospho-cyclic-AMP response element-binding protein, and upregulated the expression of brain-derived neurotrophic factor (BDNF).
29595091	3	19	theme	protein	1019:1025	arg1	levels					939:944	the levels	935:944	the levels of nitric oxide synthase and phospho-cyclic-AMP response element-binding protein	935:1025	The tempeh water extracts, especially RT-C, reduced the formation of LPS-induced reactive oxygen species, downregulated the levels of nitric oxide synthase and phospho-cyclic-AMP response element-binding protein, and upregulated the expression of brain-derived neurotrophic factor (BDNF).
29595091	2	20	theme	acid	786:789	arg1	concentrations					753:766	the highest concentrations	741:766	the highest concentrations of γ-aminobutyric acid (GABA) and anthocyanin	741:812	We analyzed the bioactive contents of tempeh extracts and evaluated the effects of tempeh water extract on lipopolysaccharide (LPS)-treated BV-2 cells.Results: The results showed that RT-C water extract had the highest concentrations of γ-aminobutyric acid (GABA) and anthocyanin.
29595091	2	21	theme	tempeh	572:577	arg1	extracts					579:586	tempeh extracts	572:586	tempeh extracts	572:586	We analyzed the bioactive contents of tempeh extracts and evaluated the effects of tempeh water extract on lipopolysaccharide (LPS)-treated BV-2 cells.Results: The results showed that RT-C water extract had the highest concentrations of γ-aminobutyric acid (GABA) and anthocyanin.
29595091	2	22	theme	γ-aminobutyric	771:784	arg1	GABA					792:795	GABA	792:795	GABA	792:795	We analyzed the bioactive contents of tempeh extracts and evaluated the effects of tempeh water extract on lipopolysaccharide (LPS)-treated BV-2 cells.Results: The results showed that RT-C water extract had the highest concentrations of γ-aminobutyric acid (GABA) and anthocyanin.
29595091	2	22	theme	γ-aminobutyric	771:784	arg1	acid					786:789	γ-aminobutyric acid	771:789	γ-aminobutyric acid (GABA)	771:796	We analyzed the bioactive contents of tempeh extracts and evaluated the effects of tempeh water extract on lipopolysaccharide (LPS)-treated BV-2 cells.Results: The results showed that RT-C water extract had the highest concentrations of γ-aminobutyric acid (GABA) and anthocyanin.
29595091	0	23	theme	Neuroprotective	0:14	arg1	effect					16:21	Neuroprotective effect	0:21	Neuroprotective effect of tempeh against lipopolysaccharide-induced damage in BV-2 microglial cells	0:98	Neuroprotective effect of tempeh against lipopolysaccharide-induced damage in BV-2 microglial cells.
29595091	3	24	theme	neurotrophic	1076:1087	arg1	factor					1089:1094	brain-derived neurotrophic factor	1062:1094	brain-derived neurotrophic factor (BDNF)	1062:1101	The tempeh water extracts, especially RT-C, reduced the formation of LPS-induced reactive oxygen species, downregulated the levels of nitric oxide synthase and phospho-cyclic-AMP response element-binding protein, and upregulated the expression of brain-derived neurotrophic factor (BDNF).
29595091	3	24	theme	neurotrophic	1076:1087	arg1	BDNF					1097:1100	BDNF	1097:1100	BDNF	1097:1100	The tempeh water extracts, especially RT-C, reduced the formation of LPS-induced reactive oxygen species, downregulated the levels of nitric oxide synthase and phospho-cyclic-AMP response element-binding protein, and upregulated the expression of brain-derived neurotrophic factor (BDNF).
29595091	4	25	theme	GABA	1180:1183	arg1	concentrations					1162:1175	the highest concentrations	1150:1175	the highest concentrations of GABA and anthocyanin	1150:1199	Discussion: Our data demonstrate that RT-C has the highest concentrations of GABA and anthocyanin, more effectively reduces oxidative stress and inflammation, and increases the expression of BDNF in LPS-induced BV-2 cells.
29595091	3	26	theme	oxide	956:960	arg1	synthase					962:969	nitric oxide synthase	949:969	nitric oxide synthase	949:969	The tempeh water extracts, especially RT-C, reduced the formation of LPS-induced reactive oxygen species, downregulated the levels of nitric oxide synthase and phospho-cyclic-AMP response element-binding protein, and upregulated the expression of brain-derived neurotrophic factor (BDNF).
29595091	0	27	theme	tempeh	26:31	arg1	effect					16:21	Neuroprotective effect	0:21	Neuroprotective effect of tempeh against lipopolysaccharide-induced damage in BV-2 microglial cells	0:98	Neuroprotective effect of tempeh against lipopolysaccharide-induced damage in BV-2 microglial cells.
29595091	1	28	theme	inflammatory	292:303	arg1	genes.Methods					305:317	inflammatory genes.Methods	292:317	inflammatory genes.Methods	292:317	Objectives: This study evaluated the bioactive composition of tempeh products and examined the effects of tempeh on BV-2 microglial cell cytotoxicity, neurotrophic effects, and expression of inflammatory genes.Methods: Tempeh products included soybean fermented by Rhizopus, soybean fermented through cocultivation with Rhizopus and Lactobacillus, and red bean fermented through cocultivation with Rhizopus and Lactobacillus (RT-C).
29595091	3	29	theme	factor	1089:1094	arg1	expression					1048:1057	the expression	1044:1057	the expression of brain-derived neurotrophic factor (BDNF)	1044:1101	The tempeh water extracts, especially RT-C, reduced the formation of LPS-induced reactive oxygen species, downregulated the levels of nitric oxide synthase and phospho-cyclic-AMP response element-binding protein, and upregulated the expression of brain-derived neurotrophic factor (BDNF).
29595091	2	30	theme	anthocyanin	802:812	arg1	concentrations					753:766	the highest concentrations	741:766	the highest concentrations of γ-aminobutyric acid (GABA) and anthocyanin	741:812	We analyzed the bioactive contents of tempeh extracts and evaluated the effects of tempeh water extract on lipopolysaccharide (LPS)-treated BV-2 cells.Results: The results showed that RT-C water extract had the highest concentrations of γ-aminobutyric acid (GABA) and anthocyanin.
29595091	1	31	theme	tempeh	207:212	arg1	effects					196:202	the effects	192:202	the effects of tempeh on BV-2 microglial cell cytotoxicity	192:249	Objectives: This study evaluated the bioactive composition of tempeh products and examined the effects of tempeh on BV-2 microglial cell cytotoxicity, neurotrophic effects, and expression of inflammatory genes.Methods: Tempeh products included soybean fermented by Rhizopus, soybean fermented through cocultivation with Rhizopus and Lactobacillus, and red bean fermented through cocultivation with Rhizopus and Lactobacillus (RT-C).
29595091	1	31	theme	tempeh	207:212	arg1	effects					265:271	neurotrophic effects	252:271	neurotrophic effects	252:271	Objectives: This study evaluated the bioactive composition of tempeh products and examined the effects of tempeh on BV-2 microglial cell cytotoxicity, neurotrophic effects, and expression of inflammatory genes.Methods: Tempeh products included soybean fermented by Rhizopus, soybean fermented through cocultivation with Rhizopus and Lactobacillus, and red bean fermented through cocultivation with Rhizopus and Lactobacillus (RT-C).
29595091	1	31	theme	tempeh	207:212	arg1	expression					278:287	expression	278:287	expression of inflammatory genes.Methods	278:317	Objectives: This study evaluated the bioactive composition of tempeh products and examined the effects of tempeh on BV-2 microglial cell cytotoxicity, neurotrophic effects, and expression of inflammatory genes.Methods: Tempeh products included soybean fermented by Rhizopus, soybean fermented through cocultivation with Rhizopus and Lactobacillus, and red bean fermented through cocultivation with Rhizopus and Lactobacillus (RT-C).
29595091	1	32	theme	genes.Methods	305:317	arg1	effects					196:202	the effects	192:202	the effects of tempeh on BV-2 microglial cell cytotoxicity	192:249	Objectives: This study evaluated the bioactive composition of tempeh products and examined the effects of tempeh on BV-2 microglial cell cytotoxicity, neurotrophic effects, and expression of inflammatory genes.Methods: Tempeh products included soybean fermented by Rhizopus, soybean fermented through cocultivation with Rhizopus and Lactobacillus, and red bean fermented through cocultivation with Rhizopus and Lactobacillus (RT-C).
29595091	1	32	theme	genes.Methods	305:317	arg1	effects					265:271	neurotrophic effects	252:271	neurotrophic effects	252:271	Objectives: This study evaluated the bioactive composition of tempeh products and examined the effects of tempeh on BV-2 microglial cell cytotoxicity, neurotrophic effects, and expression of inflammatory genes.Methods: Tempeh products included soybean fermented by Rhizopus, soybean fermented through cocultivation with Rhizopus and Lactobacillus, and red bean fermented through cocultivation with Rhizopus and Lactobacillus (RT-C).
29595091	1	32	theme	genes.Methods	305:317	arg1	expression					278:287	expression	278:287	expression of inflammatory genes.Methods	278:317	Objectives: This study evaluated the bioactive composition of tempeh products and examined the effects of tempeh on BV-2 microglial cell cytotoxicity, neurotrophic effects, and expression of inflammatory genes.Methods: Tempeh products included soybean fermented by Rhizopus, soybean fermented through cocultivation with Rhizopus and Lactobacillus, and red bean fermented through cocultivation with Rhizopus and Lactobacillus (RT-C).
29595091	1	33	from	expression	278:287	arg1	cytotoxicity					238:249	BV-2 microglial cell cytotoxicity	217:249	BV-2 microglial cell cytotoxicity	217:249	Objectives: This study evaluated the bioactive composition of tempeh products and examined the effects of tempeh on BV-2 microglial cell cytotoxicity, neurotrophic effects, and expression of inflammatory genes.Methods: Tempeh products included soybean fermented by Rhizopus, soybean fermented through cocultivation with Rhizopus and Lactobacillus, and red bean fermented through cocultivation with Rhizopus and Lactobacillus (RT-C).
29595091	4	34	theme	highest	1154:1160	arg1	concentrations					1162:1175	the highest concentrations	1150:1175	the highest concentrations of GABA and anthocyanin	1150:1199	Discussion: Our data demonstrate that RT-C has the highest concentrations of GABA and anthocyanin, more effectively reduces oxidative stress and inflammation, and increases the expression of BDNF in LPS-induced BV-2 cells.
29595091	0	35	theme	lipopolysaccharide-induced	41:66	arg1	damage					68:73	lipopolysaccharide-induced damage	41:73	lipopolysaccharide-induced damage in BV-2 microglial cells	41:98	Neuroprotective effect of tempeh against lipopolysaccharide-induced damage in BV-2 microglial cells.
29595091	1	36	from	effects	265:271	arg1	cytotoxicity					238:249	BV-2 microglial cell cytotoxicity	217:249	BV-2 microglial cell cytotoxicity	217:249	Objectives: This study evaluated the bioactive composition of tempeh products and examined the effects of tempeh on BV-2 microglial cell cytotoxicity, neurotrophic effects, and expression of inflammatory genes.Methods: Tempeh products included soybean fermented by Rhizopus, soybean fermented through cocultivation with Rhizopus and Lactobacillus, and red bean fermented through cocultivation with Rhizopus and Lactobacillus (RT-C).
29595091	3	37	theme	nitric	949:954	arg1	synthase					962:969	nitric oxide synthase	949:969	nitric oxide synthase	949:969	The tempeh water extracts, especially RT-C, reduced the formation of LPS-induced reactive oxygen species, downregulated the levels of nitric oxide synthase and phospho-cyclic-AMP response element-binding protein, and upregulated the expression of brain-derived neurotrophic factor (BDNF).
29595091	1	38	theme	BV-2	217:220	arg1	cytotoxicity					238:249	BV-2 microglial cell cytotoxicity	217:249	BV-2 microglial cell cytotoxicity	217:249	Objectives: This study evaluated the bioactive composition of tempeh products and examined the effects of tempeh on BV-2 microglial cell cytotoxicity, neurotrophic effects, and expression of inflammatory genes.Methods: Tempeh products included soybean fermented by Rhizopus, soybean fermented through cocultivation with Rhizopus and Lactobacillus, and red bean fermented through cocultivation with Rhizopus and Lactobacillus (RT-C).
29595091	1	39	theme	Tempeh	320:325	arg1	products					327:334	Tempeh products	320:334	Tempeh products	320:334	Objectives: This study evaluated the bioactive composition of tempeh products and examined the effects of tempeh on BV-2 microglial cell cytotoxicity, neurotrophic effects, and expression of inflammatory genes.Methods: Tempeh products included soybean fermented by Rhizopus, soybean fermented through cocultivation with Rhizopus and Lactobacillus, and red bean fermented through cocultivation with Rhizopus and Lactobacillus (RT-C).
29595091	4	40	contain	has	1146:1148	arg1	RT-C					1141:1144	RT-C	1141:1144	RT-C	1141:1144	Discussion: Our data demonstrate that RT-C has the highest concentrations of GABA and anthocyanin, more effectively reduces oxidative stress and inflammation, and increases the expression of BDNF in LPS-induced BV-2 cells.
29595091	4	40	contain	has	1146:1148	arg2	concentrations					1162:1175	the highest concentrations	1150:1175	the highest concentrations of GABA and anthocyanin	1150:1199	Discussion: Our data demonstrate that RT-C has the highest concentrations of GABA and anthocyanin, more effectively reduces oxidative stress and inflammation, and increases the expression of BDNF in LPS-induced BV-2 cells.
29595091	3	41	theme	response	994:1001	arg1	protein					1019:1025	phospho-cyclic-AMP response element-binding protein	975:1025	phospho-cyclic-AMP response element-binding protein	975:1025	The tempeh water extracts, especially RT-C, reduced the formation of LPS-induced reactive oxygen species, downregulated the levels of nitric oxide synthase and phospho-cyclic-AMP response element-binding protein, and upregulated the expression of brain-derived neurotrophic factor (BDNF).
29595091	1	42	theme	microglial	222:231	arg1	cytotoxicity					238:249	BV-2 microglial cell cytotoxicity	217:249	BV-2 microglial cell cytotoxicity	217:249	Objectives: This study evaluated the bioactive composition of tempeh products and examined the effects of tempeh on BV-2 microglial cell cytotoxicity, neurotrophic effects, and expression of inflammatory genes.Methods: Tempeh products included soybean fermented by Rhizopus, soybean fermented through cocultivation with Rhizopus and Lactobacillus, and red bean fermented through cocultivation with Rhizopus and Lactobacillus (RT-C).
29595091	1	43	with	cocultivation	480:492	arg1	RT-C					527:530	RT-C	527:530	RT-C	527:530	Objectives: This study evaluated the bioactive composition of tempeh products and examined the effects of tempeh on BV-2 microglial cell cytotoxicity, neurotrophic effects, and expression of inflammatory genes.Methods: Tempeh products included soybean fermented by Rhizopus, soybean fermented through cocultivation with Rhizopus and Lactobacillus, and red bean fermented through cocultivation with Rhizopus and Lactobacillus (RT-C).
29595091	1	43	with	cocultivation	480:492	arg1	Lactobacillus					512:524	Lactobacillus	512:524	Lactobacillus	512:524	Objectives: This study evaluated the bioactive composition of tempeh products and examined the effects of tempeh on BV-2 microglial cell cytotoxicity, neurotrophic effects, and expression of inflammatory genes.Methods: Tempeh products included soybean fermented by Rhizopus, soybean fermented through cocultivation with Rhizopus and Lactobacillus, and red bean fermented through cocultivation with Rhizopus and Lactobacillus (RT-C).
29595091	1	43	with	cocultivation	480:492	arg1	Rhizopus					499:506	Rhizopus	499:506	Rhizopus	499:506	Objectives: This study evaluated the bioactive composition of tempeh products and examined the effects of tempeh on BV-2 microglial cell cytotoxicity, neurotrophic effects, and expression of inflammatory genes.Methods: Tempeh products included soybean fermented by Rhizopus, soybean fermented through cocultivation with Rhizopus and Lactobacillus, and red bean fermented through cocultivation with Rhizopus and Lactobacillus (RT-C).
29595091	3	44	theme	element-binding	1003:1017	arg1	protein					1019:1025	phospho-cyclic-AMP response element-binding protein	975:1025	phospho-cyclic-AMP response element-binding protein	975:1025	The tempeh water extracts, especially RT-C, reduced the formation of LPS-induced reactive oxygen species, downregulated the levels of nitric oxide synthase and phospho-cyclic-AMP response element-binding protein, and upregulated the expression of brain-derived neurotrophic factor (BDNF).
29595091	4	45	theme	LPS-induced	1302:1312	arg1	cells					1319:1323	LPS-induced BV-2 cells	1302:1323	LPS-induced BV-2 cells	1302:1323	Discussion: Our data demonstrate that RT-C has the highest concentrations of GABA and anthocyanin, more effectively reduces oxidative stress and inflammation, and increases the expression of BDNF in LPS-induced BV-2 cells.
29595091	2	46	theme	water	723:727	arg1	extract					729:735	RT-C water extract	718:735	RT-C water extract	718:735	We analyzed the bioactive contents of tempeh extracts and evaluated the effects of tempeh water extract on lipopolysaccharide (LPS)-treated BV-2 cells.Results: The results showed that RT-C water extract had the highest concentrations of γ-aminobutyric acid (GABA) and anthocyanin.
29595091	2	47	theme	RT-C	718:721	arg1	extract					729:735	RT-C water extract	718:735	RT-C water extract	718:735	We analyzed the bioactive contents of tempeh extracts and evaluated the effects of tempeh water extract on lipopolysaccharide (LPS)-treated BV-2 cells.Results: The results showed that RT-C water extract had the highest concentrations of γ-aminobutyric acid (GABA) and anthocyanin.
29595091	1	48	theme	bioactive	138:146	arg1	composition					148:158	the bioactive composition	134:158	the bioactive composition of tempeh products	134:177	Objectives: This study evaluated the bioactive composition of tempeh products and examined the effects of tempeh on BV-2 microglial cell cytotoxicity, neurotrophic effects, and expression of inflammatory genes.Methods: Tempeh products included soybean fermented by Rhizopus, soybean fermented through cocultivation with Rhizopus and Lactobacillus, and red bean fermented through cocultivation with Rhizopus and Lactobacillus (RT-C).
29595091	1	49	with	cocultivation	402:414	arg1	Lactobacillus					434:446	Lactobacillus	434:446	Lactobacillus	434:446	Objectives: This study evaluated the bioactive composition of tempeh products and examined the effects of tempeh on BV-2 microglial cell cytotoxicity, neurotrophic effects, and expression of inflammatory genes.Methods: Tempeh products included soybean fermented by Rhizopus, soybean fermented through cocultivation with Rhizopus and Lactobacillus, and red bean fermented through cocultivation with Rhizopus and Lactobacillus (RT-C).
29595091	1	49	with	cocultivation	402:414	arg1	Rhizopus					421:428	Rhizopus	421:428	Rhizopus	421:428	Objectives: This study evaluated the bioactive composition of tempeh products and examined the effects of tempeh on BV-2 microglial cell cytotoxicity, neurotrophic effects, and expression of inflammatory genes.Methods: Tempeh products included soybean fermented by Rhizopus, soybean fermented through cocultivation with Rhizopus and Lactobacillus, and red bean fermented through cocultivation with Rhizopus and Lactobacillus (RT-C).
29595091	1	50	theme	cell	233:236	arg1	cytotoxicity					238:249	BV-2 microglial cell cytotoxicity	217:249	BV-2 microglial cell cytotoxicity	217:249	Objectives: This study evaluated the bioactive composition of tempeh products and examined the effects of tempeh on BV-2 microglial cell cytotoxicity, neurotrophic effects, and expression of inflammatory genes.Methods: Tempeh products included soybean fermented by Rhizopus, soybean fermented through cocultivation with Rhizopus and Lactobacillus, and red bean fermented through cocultivation with Rhizopus and Lactobacillus (RT-C).
29595091	3	51	theme	phospho-cyclic-AMP	975:992	arg1	protein					1019:1025	phospho-cyclic-AMP response element-binding protein	975:1025	phospho-cyclic-AMP response element-binding protein	975:1025	The tempeh water extracts, especially RT-C, reduced the formation of LPS-induced reactive oxygen species, downregulated the levels of nitric oxide synthase and phospho-cyclic-AMP response element-binding protein, and upregulated the expression of brain-derived neurotrophic factor (BDNF).
29595091	1	52	theme	red	453:455	arg1	bean					457:460	red bean	453:460	red bean fermented through cocultivation with Rhizopus and Lactobacillus (RT-C)	453:531	Objectives: This study evaluated the bioactive composition of tempeh products and examined the effects of tempeh on BV-2 microglial cell cytotoxicity, neurotrophic effects, and expression of inflammatory genes.Methods: Tempeh products included soybean fermented by Rhizopus, soybean fermented through cocultivation with Rhizopus and Lactobacillus, and red bean fermented through cocultivation with Rhizopus and Lactobacillus (RT-C).
29595091	4	53	theme	oxidative	1227:1235	arg1	stress					1237:1242	oxidative stress	1227:1242	oxidative stress	1227:1242	Discussion: Our data demonstrate that RT-C has the highest concentrations of GABA and anthocyanin, more effectively reduces oxidative stress and inflammation, and increases the expression of BDNF in LPS-induced BV-2 cells.
29595091	1	54	from	effects	196:202	arg1	cytotoxicity					238:249	BV-2 microglial cell cytotoxicity	217:249	BV-2 microglial cell cytotoxicity	217:249	Objectives: This study evaluated the bioactive composition of tempeh products and examined the effects of tempeh on BV-2 microglial cell cytotoxicity, neurotrophic effects, and expression of inflammatory genes.Methods: Tempeh products included soybean fermented by Rhizopus, soybean fermented through cocultivation with Rhizopus and Lactobacillus, and red bean fermented through cocultivation with Rhizopus and Lactobacillus (RT-C).
29595091	3	55	theme	synthase	962:969	arg1	levels					939:944	the levels	935:944	the levels of nitric oxide synthase and phospho-cyclic-AMP response element-binding protein	935:1025	The tempeh water extracts, especially RT-C, reduced the formation of LPS-induced reactive oxygen species, downregulated the levels of nitric oxide synthase and phospho-cyclic-AMP response element-binding protein, and upregulated the expression of brain-derived neurotrophic factor (BDNF).
29595091	0	56	theme	microglial	83:92	arg1	cells					94:98	BV-2 microglial cells	78:98	BV-2 microglial cells	78:98	Neuroprotective effect of tempeh against lipopolysaccharide-induced damage in BV-2 microglial cells.
29595091	4	57	theme	BV-2	1314:1317	arg1	cells					1319:1323	LPS-induced BV-2 cells	1302:1323	LPS-induced BV-2 cells	1302:1323	Discussion: Our data demonstrate that RT-C has the highest concentrations of GABA and anthocyanin, more effectively reduces oxidative stress and inflammation, and increases the expression of BDNF in LPS-induced BV-2 cells.
29595091	4	58	theme	anthocyanin	1189:1199	arg1	concentrations					1162:1175	the highest concentrations	1150:1175	the highest concentrations of GABA and anthocyanin	1150:1199	Discussion: Our data demonstrate that RT-C has the highest concentrations of GABA and anthocyanin, more effectively reduces oxidative stress and inflammation, and increases the expression of BDNF in LPS-induced BV-2 cells.
29595091	0	59	theme	BV-2	78:81	arg1	cells					94:98	BV-2 microglial cells	78:98	BV-2 microglial cells	78:98	Neuroprotective effect of tempeh against lipopolysaccharide-induced damage in BV-2 microglial cells.
29595091	3	60	theme	tempeh	819:824	arg1	extracts					832:839	The tempeh water extracts	815:839	The tempeh water extracts	815:839	The tempeh water extracts, especially RT-C, reduced the formation of LPS-induced reactive oxygen species, downregulated the levels of nitric oxide synthase and phospho-cyclic-AMP response element-binding protein, and upregulated the expression of brain-derived neurotrophic factor (BDNF).
29595091	3	60	theme	tempeh	819:824	arg1	RT-C					853:856	RT-C	853:856	especially RT-C	842:856	The tempeh water extracts, especially RT-C, reduced the formation of LPS-induced reactive oxygen species, downregulated the levels of nitric oxide synthase and phospho-cyclic-AMP response element-binding protein, and upregulated the expression of brain-derived neurotrophic factor (BDNF).
29595091	2	61	theme	highest	745:751	arg1	concentrations					753:766	the highest concentrations	741:766	the highest concentrations of γ-aminobutyric acid (GABA) and anthocyanin	741:812	We analyzed the bioactive contents of tempeh extracts and evaluated the effects of tempeh water extract on lipopolysaccharide (LPS)-treated BV-2 cells.Results: The results showed that RT-C water extract had the highest concentrations of γ-aminobutyric acid (GABA) and anthocyanin.
29595091	1	62	theme	tempeh	163:168	arg1	products					170:177	tempeh products	163:177	tempeh products	163:177	Objectives: This study evaluated the bioactive composition of tempeh products and examined the effects of tempeh on BV-2 microglial cell cytotoxicity, neurotrophic effects, and expression of inflammatory genes.Methods: Tempeh products included soybean fermented by Rhizopus, soybean fermented through cocultivation with Rhizopus and Lactobacillus, and red bean fermented through cocultivation with Rhizopus and Lactobacillus (RT-C).
29595091	3	63	theme	oxygen	905:910	arg1	species					912:918	LPS-induced reactive oxygen species	884:918	LPS-induced reactive oxygen species	884:918	The tempeh water extracts, especially RT-C, reduced the formation of LPS-induced reactive oxygen species, downregulated the levels of nitric oxide synthase and phospho-cyclic-AMP response element-binding protein, and upregulated the expression of brain-derived neurotrophic factor (BDNF).
29595091	1	64	theme	neurotrophic	252:263	arg1	effects					265:271	neurotrophic effects	252:271	neurotrophic effects	252:271	Objectives: This study evaluated the bioactive composition of tempeh products and examined the effects of tempeh on BV-2 microglial cell cytotoxicity, neurotrophic effects, and expression of inflammatory genes.Methods: Tempeh products included soybean fermented by Rhizopus, soybean fermented through cocultivation with Rhizopus and Lactobacillus, and red bean fermented through cocultivation with Rhizopus and Lactobacillus (RT-C).
31364965	12	0	theme	aspartic	1685:1692	arg1	acid					1694:1697	aspartic acid	1685:1697	aspartic acid	1685:1697	The cell-wall peptidoglycan of strain YLB-02T contained glutamic acid, alanine, aspartic acid, lysine and ornithine, but no meso-diaminopimelic acid, while strain YLB-04T contained meso-diaminopimelic acid, glutamic acid, alanine, aspartic acid, lysine and ornithine.
31364965	13	1	dep	phospholipids	1946:1958	arg1	phospholipids					1946:1958	two unidentified phospholipids	1929:1958	two unidentified phospholipids (PL1 and PL2)	1929:1972	In addition to diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), the polar lipids of strain YLB-02T also consisted of an unidentified glycolipid (GL), two unidentified polar lipids (L1 and L2) and two unidentified phospholipids (PL1 and PL2), and those of strain YLB-04T also consisted of phosphatidylethanolamine (PE) and an unidentified phospholipid (PL).
31364965	13	1	dep	phospholipids	1946:1958	arg1	PL2					1969:1971	PL2	1969:1971	PL2	1969:1971	In addition to diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), the polar lipids of strain YLB-02T also consisted of an unidentified glycolipid (GL), two unidentified polar lipids (L1 and L2) and two unidentified phospholipids (PL1 and PL2), and those of strain YLB-04T also consisted of phosphatidylethanolamine (PE) and an unidentified phospholipid (PL).
31364965	13	1	dep	phospholipids	1946:1958	arg1	PL1					1961:1963	PL1	1961:1963	PL1	1961:1963	In addition to diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), the polar lipids of strain YLB-02T also consisted of an unidentified glycolipid (GL), two unidentified polar lipids (L1 and L2) and two unidentified phospholipids (PL1 and PL2), and those of strain YLB-04T also consisted of phosphatidylethanolamine (PE) and an unidentified phospholipid (PL).
31364965	12	2	theme	aspartic	1534:1541	arg1	acid					1543:1546	aspartic acid	1534:1546	aspartic acid	1534:1546	The cell-wall peptidoglycan of strain YLB-02T contained glutamic acid, alanine, aspartic acid, lysine and ornithine, but no meso-diaminopimelic acid, while strain YLB-04T contained meso-diaminopimelic acid, glutamic acid, alanine, aspartic acid, lysine and ornithine.
31364965	7	3	theme	Bacillus	873:880	arg1	SYP-B691T					899:907	Bacillus notoginsengisoli SYP-B691T	873:907	Bacillus notoginsengisoli SYP-B691T	873:907	Strain YLB-04T showed the highest sequence similarity of 97.4 % with Bacillus notoginsengisoli SYP-B691T.
31364965	6	4	with	%	715:715	arg1	CL-MP28T					700:707	Oceanobacillus profundus CL-MP28T	675:707	Oceanobacillus profundus CL-MP28T	675:707	Strain YLB-02T showed similarities of 96.9 % with Ornithinibacillus contaminans CCUG 53201T, 96.3 % with Oceanobacillus profundus CL-MP28T, 96.1 % with Oceanobacillus halophilus J8BT and 95.7 % with Oceanobacillus bengalensis Ma-21T.
31364965	6	4	with	%	715:715	arg1	Ma-21T					796:801	Oceanobacillus bengalensis Ma-21T	769:801	Oceanobacillus bengalensis Ma-21T	769:801	Strain YLB-02T showed similarities of 96.9 % with Ornithinibacillus contaminans CCUG 53201T, 96.3 % with Oceanobacillus profundus CL-MP28T, 96.1 % with Oceanobacillus halophilus J8BT and 95.7 % with Oceanobacillus bengalensis Ma-21T.
31364965	6	4	with	%	715:715	arg1	53201T					655:660	Ornithinibacillus contaminans CCUG 53201T	620:660	Ornithinibacillus contaminans CCUG 53201T	620:660	Strain YLB-02T showed similarities of 96.9 % with Ornithinibacillus contaminans CCUG 53201T, 96.3 % with Oceanobacillus profundus CL-MP28T, 96.1 % with Oceanobacillus halophilus J8BT and 95.7 % with Oceanobacillus bengalensis Ma-21T.
31364965	6	4	with	%	715:715	arg1	J8BT					748:751	Oceanobacillus halophilus J8BT	722:751	Oceanobacillus halophilus J8BT	722:751	Strain YLB-02T showed similarities of 96.9 % with Ornithinibacillus contaminans CCUG 53201T, 96.3 % with Oceanobacillus profundus CL-MP28T, 96.1 % with Oceanobacillus halophilus J8BT and 95.7 % with Oceanobacillus bengalensis Ma-21T.
31364965	14	5	theme	piezotolerans	2204:2216	arg1	sp					2218:2219	piezotolerans sp	2204:2219	piezotolerans sp	2204:2219	Based on phenotypic, genotypic and chemotaxonomic characteristics, two novel species are proposed, Oceanobacillus piezotolerans sp.
31364965	7	6	theme	%	866:866	arg1	similarity					847:856	the highest sequence similarity	826:856	the highest sequence similarity of 97.4 % with Bacillus notoginsengisoli SYP-B691T	826:907	Strain YLB-04T showed the highest sequence similarity of 97.4 % with Bacillus notoginsengisoli SYP-B691T.
31364965	2	7	theme	deep-sea	125:132	arg1	samples					143:149	deep-sea sediment samples	125:149	deep-sea sediment samples of Yap Trench	125:163	nov., from deep-sea sediment samples of Yap Trench.
31364965	8	8	with	YLB-04T	1023:1029	arg1	strains					1055:1061	their related type strains	1036:1061	their related type strains	1036:1061	The average nucleotide identity (ANI) and the DNA-DNA hybridisation (DDH) estimate values for strain YLB-02T and YLB-04T with their related type strains were below the respective threshold for species differentiation.
31364965	6	9	theme	Oceanobacillus	769:782	arg1	Ma-21T					796:801	Oceanobacillus bengalensis Ma-21T	769:801	Oceanobacillus bengalensis Ma-21T	769:801	Strain YLB-02T showed similarities of 96.9 % with Ornithinibacillus contaminans CCUG 53201T, 96.3 % with Oceanobacillus profundus CL-MP28T, 96.1 % with Oceanobacillus halophilus J8BT and 95.7 % with Oceanobacillus bengalensis Ma-21T.
31364965	7	10	theme	sequence	838:845	arg1	similarity					847:856	the highest sequence similarity	826:856	the highest sequence similarity of 97.4 % with Bacillus notoginsengisoli SYP-B691T	826:907	Strain YLB-04T showed the highest sequence similarity of 97.4 % with Bacillus notoginsengisoli SYP-B691T.
31364965	12	11	theme	meso-diaminopimelic	1635:1653	arg1	acid					1655:1658	meso-diaminopimelic acid	1635:1658	meso-diaminopimelic acid	1635:1658	The cell-wall peptidoglycan of strain YLB-02T contained glutamic acid, alanine, aspartic acid, lysine and ornithine, but no meso-diaminopimelic acid, while strain YLB-04T contained meso-diaminopimelic acid, glutamic acid, alanine, aspartic acid, lysine and ornithine.
31364965	14	12	theme	chemotaxonomic	2125:2138	arg1	characteristics					2140:2154	phenotypic, genotypic and chemotaxonomic characteristics	2099:2154	phenotypic, genotypic and chemotaxonomic characteristics	2099:2154	Based on phenotypic, genotypic and chemotaxonomic characteristics, two novel species are proposed, Oceanobacillus piezotolerans sp.
31364965	14	13	theme	novel	2161:2165	arg1	species					2167:2173	two novel species	2157:2173	two novel species	2157:2173	Based on phenotypic, genotypic and chemotaxonomic characteristics, two novel species are proposed, Oceanobacillus piezotolerans sp.
31364965	2	14	from	samples	143:149	arg1	nov.					114:117	nov.	114:117	nov.	114:117	nov., from deep-sea sediment samples of Yap Trench.
31364965	3	15	theme	located	273:279	arg1	sediments					249:257	the deep-sea sediments	236:257	the deep-sea sediments of Yap Trench located in the Pacific Ocean	236:300	Two novel strains, designated YLB-02T and YLB-04T, were isolated from the deep-sea sediments of Yap Trench located in the Pacific Ocean.
31364965	12	16	theme	strain	1485:1490	arg1	YLB-02T					1492:1498	strain YLB-02T	1485:1498	strain YLB-02T	1485:1498	The cell-wall peptidoglycan of strain YLB-02T contained glutamic acid, alanine, aspartic acid, lysine and ornithine, but no meso-diaminopimelic acid, while strain YLB-04T contained meso-diaminopimelic acid, glutamic acid, alanine, aspartic acid, lysine and ornithine.
31364965	3	17	theme	Yap	262:264	arg1	Trench					266:271	Yap Trench	262:271	Yap Trench	262:271	Two novel strains, designated YLB-02T and YLB-04T, were isolated from the deep-sea sediments of Yap Trench located in the Pacific Ocean.
31364965	10	18	theme	cellular	1225:1232	arg1	iso-C14 					1269:1276	iso-C14 	1269:1276	iso-C14 : 0, iso-C15 : 0, iso-C16 : 0 and C16 : 1ω7c alcohol	1269:1328	The predominant (>10 %) cellular fatty acids of strain YLB-02T were iso-C14 : 0, iso-C15 : 0, iso-C16 : 0 and C16 : 1ω7c alcohol, and those of strain YLB-04T were C16 : 0, iso-C15 : 0, anteiso-C15 : 0 and C18 : 0.
31364965	10	18	theme	cellular	1225:1232	arg1	acids					1240:1244	The predominant (>10 %) cellular fatty acids	1201:1244	The predominant (>10 %) cellular fatty acids of strain YLB-02T	1201:1262	The predominant (>10 %) cellular fatty acids of strain YLB-02T were iso-C14 : 0, iso-C15 : 0, iso-C16 : 0 and C16 : 1ω7c alcohol, and those of strain YLB-04T were C16 : 0, iso-C15 : 0, anteiso-C15 : 0 and C18 : 0.
31364965	3	19	from	Ocean	296:300	arg1	located					273:279	located	273:279	located	273:279	Two novel strains, designated YLB-02T and YLB-04T, were isolated from the deep-sea sediments of Yap Trench located in the Pacific Ocean.
31364965	10	20	dep	iso-C14 	1269:1276	arg1	C16 					1311:1314	C16 	1311:1314	C16 	1311:1314	The predominant (>10 %) cellular fatty acids of strain YLB-02T were iso-C14 : 0, iso-C15 : 0, iso-C16 : 0 and C16 : 1ω7c alcohol, and those of strain YLB-04T were C16 : 0, iso-C15 : 0, anteiso-C15 : 0 and C18 : 0.
31364965	10	20	dep	iso-C14 	1269:1276	arg1	alcohol					1322:1328	 1ω7c alcohol	1316:1328	iso-C14 : 0, iso-C15 : 0, iso-C16 : 0 and C16 : 1ω7c alcohol	1269:1328	The predominant (>10 %) cellular fatty acids of strain YLB-02T were iso-C14 : 0, iso-C15 : 0, iso-C16 : 0 and C16 : 1ω7c alcohol, and those of strain YLB-04T were C16 : 0, iso-C15 : 0, anteiso-C15 : 0 and C18 : 0.
31364965	10	20	dep	iso-C14 	1269:1276	arg1	 0					1278:1279	 0	1278:1279	 0	1278:1279	The predominant (>10 %) cellular fatty acids of strain YLB-02T were iso-C14 : 0, iso-C15 : 0, iso-C16 : 0 and C16 : 1ω7c alcohol, and those of strain YLB-04T were C16 : 0, iso-C15 : 0, anteiso-C15 : 0 and C18 : 0.
31364965	10	20	dep	iso-C14 	1269:1276	arg1	 0					1304:1305	 0	1304:1305	 0	1304:1305	The predominant (>10 %) cellular fatty acids of strain YLB-02T were iso-C14 : 0, iso-C15 : 0, iso-C16 : 0 and C16 : 1ω7c alcohol, and those of strain YLB-04T were C16 : 0, iso-C15 : 0, anteiso-C15 : 0 and C18 : 0.
31364965	10	20	dep	iso-C14 	1269:1276	arg1	 0					1291:1292	 0	1291:1292	 0	1291:1292	The predominant (>10 %) cellular fatty acids of strain YLB-02T were iso-C14 : 0, iso-C15 : 0, iso-C16 : 0 and C16 : 1ω7c alcohol, and those of strain YLB-04T were C16 : 0, iso-C15 : 0, anteiso-C15 : 0 and C18 : 0.
31364965	12	21	theme	strain	1610:1615	arg1	YLB-04T					1617:1623	strain YLB-04T	1610:1623	strain YLB-04T	1610:1623	The cell-wall peptidoglycan of strain YLB-02T contained glutamic acid, alanine, aspartic acid, lysine and ornithine, but no meso-diaminopimelic acid, while strain YLB-04T contained meso-diaminopimelic acid, glutamic acid, alanine, aspartic acid, lysine and ornithine.
31364965	6	22	theme	contaminans	638:648	arg1	53201T					655:660	Ornithinibacillus contaminans CCUG 53201T	620:660	Ornithinibacillus contaminans CCUG 53201T	620:660	Strain YLB-02T showed similarities of 96.9 % with Ornithinibacillus contaminans CCUG 53201T, 96.3 % with Oceanobacillus profundus CL-MP28T, 96.1 % with Oceanobacillus halophilus J8BT and 95.7 % with Oceanobacillus bengalensis Ma-21T.
31364965	0	23	theme	Oceanobacillus	45:58	arg1	sp					74:75	Oceanobacillus piezotolerans sp	45:75	Two novel species of the family Bacillaceae: Oceanobacillus piezotolerans sp.	0:76	Two novel species of the family Bacillaceae: Oceanobacillus piezotolerans sp.
31364965	10	24	theme	strain	1249:1254	arg1	YLB-02T					1256:1262	strain YLB-02T	1249:1262	strain YLB-02T	1249:1262	The predominant (>10 %) cellular fatty acids of strain YLB-02T were iso-C14 : 0, iso-C15 : 0, iso-C16 : 0 and C16 : 1ω7c alcohol, and those of strain YLB-04T were C16 : 0, iso-C15 : 0, anteiso-C15 : 0 and C18 : 0.
31364965	15	25	theme	=MCCC	2241:2245	arg1	YLB-02T					2232:2238	YLB-02T	2232:2238	YLB-02T (=MCCC 1A12699T=JCM 32870T)	2232:2266	nov. with YLB-02T (=MCCC 1A12699T=JCM 32870T) and Bacillus piezotolerans sp.
31364965	15	25	theme	=MCCC	2241:2245	arg1	32870T					2260:2265	=MCCC 1A12699T=JCM 32870T	2241:2265	=MCCC 1A12699T=JCM 32870T	2241:2265	nov. with YLB-02T (=MCCC 1A12699T=JCM 32870T) and Bacillus piezotolerans sp.
31364965	12	26	theme	meso-diaminopimelic	1578:1596	arg1	acid					1598:1601	no meso-diaminopimelic acid	1575:1601	no meso-diaminopimelic acid	1575:1601	The cell-wall peptidoglycan of strain YLB-02T contained glutamic acid, alanine, aspartic acid, lysine and ornithine, but no meso-diaminopimelic acid, while strain YLB-04T contained meso-diaminopimelic acid, glutamic acid, alanine, aspartic acid, lysine and ornithine.
31364965	16	27	theme	=MCCC	2318:2322	arg1	32872T					2337:2342	=MCCC 1A12711T=JCM 32872T	2318:2342	=MCCC 1A12711T=JCM 32872T	2318:2342	nov. with YLB-04T (=MCCC 1A12711T=JCM 32872T) as the type strains.
31364965	16	27	theme	=MCCC	2318:2322	arg1	YLB-04T					2309:2315	YLB-04T	2309:2315	YLB-04T (=MCCC 1A12711T=JCM 32872T)	2309:2343	nov. with YLB-04T (=MCCC 1A12711T=JCM 32872T) as the type strains.
31364965	5	28	theme	genus	498:502	arg1	Oceanobacillus					504:517	the genus Oceanobacillus	494:517	the genus Oceanobacillus	494:517	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain YLB-02T belonged to the genus Oceanobacillus and strain YLB-04T belonged to the genus Bacillus.
31364965	13	29	theme	polar	1900:1904	arg1	L2					1921:1922	L2	1921:1922	L2	1921:1922	In addition to diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), the polar lipids of strain YLB-02T also consisted of an unidentified glycolipid (GL), two unidentified polar lipids (L1 and L2) and two unidentified phospholipids (PL1 and PL2), and those of strain YLB-04T also consisted of phosphatidylethanolamine (PE) and an unidentified phospholipid (PL).
31364965	13	29	theme	polar	1900:1904	arg1	L1					1914:1915	L1	1914:1915	L1	1914:1915	In addition to diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), the polar lipids of strain YLB-02T also consisted of an unidentified glycolipid (GL), two unidentified polar lipids (L1 and L2) and two unidentified phospholipids (PL1 and PL2), and those of strain YLB-04T also consisted of phosphatidylethanolamine (PE) and an unidentified phospholipid (PL).
31364965	13	29	theme	polar	1900:1904	arg1	lipids					1906:1911	two unidentified polar lipids	1883:1911	two unidentified polar lipids (L1 and L2)	1883:1923	In addition to diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), the polar lipids of strain YLB-02T also consisted of an unidentified glycolipid (GL), two unidentified polar lipids (L1 and L2) and two unidentified phospholipids (PL1 and PL2), and those of strain YLB-04T also consisted of phosphatidylethanolamine (PE) and an unidentified phospholipid (PL).
31364965	9	30	dep	strains	1148:1154	arg1	strains					1148:1154	strains YLB-02T and YLB-04T	1148:1174	strains YLB-02T and YLB-04T	1148:1174	The G+C contents of strains YLB-02T and YLB-04T were 37.3 and 45.4 mol%.
31364965	9	30	dep	strains	1148:1154	arg1	YLB-04T					1168:1174	YLB-04T	1168:1174	YLB-04T	1168:1174	The G+C contents of strains YLB-02T and YLB-04T were 37.3 and 45.4 mol%.
31364965	9	30	dep	strains	1148:1154	arg1	YLB-02T					1156:1162	YLB-02T	1156:1162	YLB-02T	1156:1162	The G+C contents of strains YLB-02T and YLB-04T were 37.3 and 45.4 mol%.
31364965	6	31	with	%	613:613	arg1	CL-MP28T					700:707	Oceanobacillus profundus CL-MP28T	675:707	Oceanobacillus profundus CL-MP28T	675:707	Strain YLB-02T showed similarities of 96.9 % with Ornithinibacillus contaminans CCUG 53201T, 96.3 % with Oceanobacillus profundus CL-MP28T, 96.1 % with Oceanobacillus halophilus J8BT and 95.7 % with Oceanobacillus bengalensis Ma-21T.
31364965	6	31	with	%	613:613	arg1	Ma-21T					796:801	Oceanobacillus bengalensis Ma-21T	769:801	Oceanobacillus bengalensis Ma-21T	769:801	Strain YLB-02T showed similarities of 96.9 % with Ornithinibacillus contaminans CCUG 53201T, 96.3 % with Oceanobacillus profundus CL-MP28T, 96.1 % with Oceanobacillus halophilus J8BT and 95.7 % with Oceanobacillus bengalensis Ma-21T.
31364965	6	31	with	%	613:613	arg1	53201T					655:660	Ornithinibacillus contaminans CCUG 53201T	620:660	Ornithinibacillus contaminans CCUG 53201T	620:660	Strain YLB-02T showed similarities of 96.9 % with Ornithinibacillus contaminans CCUG 53201T, 96.3 % with Oceanobacillus profundus CL-MP28T, 96.1 % with Oceanobacillus halophilus J8BT and 95.7 % with Oceanobacillus bengalensis Ma-21T.
31364965	6	31	with	%	613:613	arg1	J8BT					748:751	Oceanobacillus halophilus J8BT	722:751	Oceanobacillus halophilus J8BT	722:751	Strain YLB-02T showed similarities of 96.9 % with Ornithinibacillus contaminans CCUG 53201T, 96.3 % with Oceanobacillus profundus CL-MP28T, 96.1 % with Oceanobacillus halophilus J8BT and 95.7 % with Oceanobacillus bengalensis Ma-21T.
31364965	8	32	theme	type	1050:1053	arg1	strains					1055:1061	their related type strains	1036:1061	their related type strains	1036:1061	The average nucleotide identity (ANI) and the DNA-DNA hybridisation (DDH) estimate values for strain YLB-02T and YLB-04T with their related type strains were below the respective threshold for species differentiation.
31364965	13	33	theme	unidentified	1853:1864	arg1	GL					1878:1879	GL	1878:1879	GL	1878:1879	In addition to diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), the polar lipids of strain YLB-02T also consisted of an unidentified glycolipid (GL), two unidentified polar lipids (L1 and L2) and two unidentified phospholipids (PL1 and PL2), and those of strain YLB-04T also consisted of phosphatidylethanolamine (PE) and an unidentified phospholipid (PL).
31364965	13	33	theme	unidentified	1853:1864	arg1	glycolipid					1866:1875	an unidentified glycolipid	1850:1875	an unidentified glycolipid (GL)	1850:1880	In addition to diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), the polar lipids of strain YLB-02T also consisted of an unidentified glycolipid (GL), two unidentified polar lipids (L1 and L2) and two unidentified phospholipids (PL1 and PL2), and those of strain YLB-04T also consisted of phosphatidylethanolamine (PE) and an unidentified phospholipid (PL).
31364965	13	34	theme	unidentified	2058:2069	arg1	PL					2085:2086	PL	2085:2086	PL	2085:2086	In addition to diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), the polar lipids of strain YLB-02T also consisted of an unidentified glycolipid (GL), two unidentified polar lipids (L1 and L2) and two unidentified phospholipids (PL1 and PL2), and those of strain YLB-04T also consisted of phosphatidylethanolamine (PE) and an unidentified phospholipid (PL).
31364965	13	34	theme	unidentified	2058:2069	arg1	phospholipid					2071:2082	an unidentified phospholipid	2055:2082	an unidentified phospholipid (PL)	2055:2087	In addition to diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), the polar lipids of strain YLB-02T also consisted of an unidentified glycolipid (GL), two unidentified polar lipids (L1 and L2) and two unidentified phospholipids (PL1 and PL2), and those of strain YLB-04T also consisted of phosphatidylethanolamine (PE) and an unidentified phospholipid (PL).
31364965	13	35	theme	strain	1817:1822	arg1	YLB-02T					1824:1830	strain YLB-02T	1817:1830	strain YLB-02T	1817:1830	In addition to diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), the polar lipids of strain YLB-02T also consisted of an unidentified glycolipid (GL), two unidentified polar lipids (L1 and L2) and two unidentified phospholipids (PL1 and PL2), and those of strain YLB-04T also consisted of phosphatidylethanolamine (PE) and an unidentified phospholipid (PL).
31364965	8	36	theme	average	914:920	arg1	ANI					943:945	ANI	943:945	ANI	943:945	The average nucleotide identity (ANI) and the DNA-DNA hybridisation (DDH) estimate values for strain YLB-02T and YLB-04T with their related type strains were below the respective threshold for species differentiation.
31364965	8	36	theme	average	914:920	arg1	identity					933:940	The average nucleotide identity	910:940	The average nucleotide identity (ANI)	910:946	The average nucleotide identity (ANI) and the DNA-DNA hybridisation (DDH) estimate values for strain YLB-02T and YLB-04T with their related type strains were below the respective threshold for species differentiation.
31364965	3	37	theme	novel	170:174	arg1	strains					176:182	Two novel strains	166:182	Two novel strains	166:182	Two novel strains, designated YLB-02T and YLB-04T, were isolated from the deep-sea sediments of Yap Trench located in the Pacific Ocean.
31364965	10	38	dep	predominant	1205:1215	arg1	%					1222:1222	>10 %	1218:1222	>10 %	1218:1222	The predominant (>10 %) cellular fatty acids of strain YLB-02T were iso-C14 : 0, iso-C15 : 0, iso-C16 : 0 and C16 : 1ω7c alcohol, and those of strain YLB-04T were C16 : 0, iso-C15 : 0, anteiso-C15 : 0 and C18 : 0.
31364965	1	39	theme	piezotolerans	96:108	arg1	sp					110:111	Bacillus piezotolerans sp	87:111	Bacillus piezotolerans sp	87:111	nov. and Bacillus piezotolerans sp.
31364965	6	40	theme	Ornithinibacillus	620:636	arg1	53201T					655:660	Ornithinibacillus contaminans CCUG 53201T	620:660	Ornithinibacillus contaminans CCUG 53201T	620:660	Strain YLB-02T showed similarities of 96.9 % with Ornithinibacillus contaminans CCUG 53201T, 96.3 % with Oceanobacillus profundus CL-MP28T, 96.1 % with Oceanobacillus halophilus J8BT and 95.7 % with Oceanobacillus bengalensis Ma-21T.
31364965	2	41	theme	Trench	158:163	arg1	samples					143:149	deep-sea sediment samples	125:149	deep-sea sediment samples of Yap Trench	125:163	nov., from deep-sea sediment samples of Yap Trench.
31364965	13	42	theme	polar	1801:1805	arg1	lipids					1807:1812	the polar lipids	1797:1812	the polar lipids of strain YLB-02T	1797:1830	In addition to diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), the polar lipids of strain YLB-02T also consisted of an unidentified glycolipid (GL), two unidentified polar lipids (L1 and L2) and two unidentified phospholipids (PL1 and PL2), and those of strain YLB-04T also consisted of phosphatidylethanolamine (PE) and an unidentified phospholipid (PL).
31364965	16	43	with	nov.	2299:2302	arg1	32872T					2337:2342	=MCCC 1A12711T=JCM 32872T	2318:2342	=MCCC 1A12711T=JCM 32872T	2318:2342	nov. with YLB-04T (=MCCC 1A12711T=JCM 32872T) as the type strains.
31364965	16	43	with	nov.	2299:2302	arg1	YLB-04T					2309:2315	YLB-04T	2309:2315	YLB-04T (=MCCC 1A12711T=JCM 32872T)	2309:2343	nov. with YLB-04T (=MCCC 1A12711T=JCM 32872T) as the type strains.
31364965	10	44	dep	C16 	1364:1367	arg1	 0					1411:1412	 0	1411:1412	 0	1411:1412	The predominant (>10 %) cellular fatty acids of strain YLB-02T were iso-C14 : 0, iso-C15 : 0, iso-C16 : 0 and C16 : 1ω7c alcohol, and those of strain YLB-04T were C16 : 0, iso-C15 : 0, anteiso-C15 : 0 and C18 : 0.
31364965	6	45	with	%	668:668	arg1	CL-MP28T					700:707	Oceanobacillus profundus CL-MP28T	675:707	Oceanobacillus profundus CL-MP28T	675:707	Strain YLB-02T showed similarities of 96.9 % with Ornithinibacillus contaminans CCUG 53201T, 96.3 % with Oceanobacillus profundus CL-MP28T, 96.1 % with Oceanobacillus halophilus J8BT and 95.7 % with Oceanobacillus bengalensis Ma-21T.
31364965	6	45	with	%	668:668	arg1	Ma-21T					796:801	Oceanobacillus bengalensis Ma-21T	769:801	Oceanobacillus bengalensis Ma-21T	769:801	Strain YLB-02T showed similarities of 96.9 % with Ornithinibacillus contaminans CCUG 53201T, 96.3 % with Oceanobacillus profundus CL-MP28T, 96.1 % with Oceanobacillus halophilus J8BT and 95.7 % with Oceanobacillus bengalensis Ma-21T.
31364965	6	45	with	%	668:668	arg1	53201T					655:660	Ornithinibacillus contaminans CCUG 53201T	620:660	Ornithinibacillus contaminans CCUG 53201T	620:660	Strain YLB-02T showed similarities of 96.9 % with Ornithinibacillus contaminans CCUG 53201T, 96.3 % with Oceanobacillus profundus CL-MP28T, 96.1 % with Oceanobacillus halophilus J8BT and 95.7 % with Oceanobacillus bengalensis Ma-21T.
31364965	6	45	with	%	668:668	arg1	J8BT					748:751	Oceanobacillus halophilus J8BT	722:751	Oceanobacillus halophilus J8BT	722:751	Strain YLB-02T showed similarities of 96.9 % with Ornithinibacillus contaminans CCUG 53201T, 96.3 % with Oceanobacillus profundus CL-MP28T, 96.1 % with Oceanobacillus halophilus J8BT and 95.7 % with Oceanobacillus bengalensis Ma-21T.
31364965	0	46	theme	Bacillaceae	32:42	arg1	species					10:16	Two novel species	0:16	Two novel species of the family Bacillaceae: Oceanobacillus piezotolerans sp.	0:76	Two novel species of the family Bacillaceae: Oceanobacillus piezotolerans sp.
31364965	8	47	theme	species	1103:1109	arg1	differentiation					1111:1125	species differentiation	1103:1125	species differentiation	1103:1125	The average nucleotide identity (ANI) and the DNA-DNA hybridisation (DDH) estimate values for strain YLB-02T and YLB-04T with their related type strains were below the respective threshold for species differentiation.
31364965	4	48	theme	strains	316:322	arg1	Cells					303:307	Cells	303:307	Cells of the strains	303:322	Cells of the strains were Gram-stain-positive, oxidase- and catalase-positive and rod-shaped.
31364965	13	49	dep	lipids	1906:1911	arg1	L2					1921:1922	L2	1921:1922	L2	1921:1922	In addition to diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), the polar lipids of strain YLB-02T also consisted of an unidentified glycolipid (GL), two unidentified polar lipids (L1 and L2) and two unidentified phospholipids (PL1 and PL2), and those of strain YLB-04T also consisted of phosphatidylethanolamine (PE) and an unidentified phospholipid (PL).
31364965	13	49	dep	lipids	1906:1911	arg1	L1					1914:1915	L1	1914:1915	L1	1914:1915	In addition to diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), the polar lipids of strain YLB-02T also consisted of an unidentified glycolipid (GL), two unidentified polar lipids (L1 and L2) and two unidentified phospholipids (PL1 and PL2), and those of strain YLB-04T also consisted of phosphatidylethanolamine (PE) and an unidentified phospholipid (PL).
31364965	13	49	dep	lipids	1906:1911	arg1	lipids					1906:1911	two unidentified polar lipids	1883:1911	two unidentified polar lipids (L1 and L2)	1883:1923	In addition to diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), the polar lipids of strain YLB-02T also consisted of an unidentified glycolipid (GL), two unidentified polar lipids (L1 and L2) and two unidentified phospholipids (PL1 and PL2), and those of strain YLB-04T also consisted of phosphatidylethanolamine (PE) and an unidentified phospholipid (PL).
31364965	13	50	theme	unidentified	1887:1898	arg1	L2					1921:1922	L2	1921:1922	L2	1921:1922	In addition to diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), the polar lipids of strain YLB-02T also consisted of an unidentified glycolipid (GL), two unidentified polar lipids (L1 and L2) and two unidentified phospholipids (PL1 and PL2), and those of strain YLB-04T also consisted of phosphatidylethanolamine (PE) and an unidentified phospholipid (PL).
31364965	13	50	theme	unidentified	1887:1898	arg1	L1					1914:1915	L1	1914:1915	L1	1914:1915	In addition to diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), the polar lipids of strain YLB-02T also consisted of an unidentified glycolipid (GL), two unidentified polar lipids (L1 and L2) and two unidentified phospholipids (PL1 and PL2), and those of strain YLB-04T also consisted of phosphatidylethanolamine (PE) and an unidentified phospholipid (PL).
31364965	13	50	theme	unidentified	1887:1898	arg1	lipids					1906:1911	two unidentified polar lipids	1883:1911	two unidentified polar lipids (L1 and L2)	1883:1923	In addition to diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), the polar lipids of strain YLB-02T also consisted of an unidentified glycolipid (GL), two unidentified polar lipids (L1 and L2) and two unidentified phospholipids (PL1 and PL2), and those of strain YLB-04T also consisted of phosphatidylethanolamine (PE) and an unidentified phospholipid (PL).
31364965	5	51	theme	16S	428:430	arg1	sequences					442:450	16S rRNA gene sequences	428:450	16S rRNA gene sequences	428:450	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain YLB-02T belonged to the genus Oceanobacillus and strain YLB-04T belonged to the genus Bacillus.
31364965	13	52	theme	strain	1988:1993	arg1	YLB-04T					1995:2001	strain YLB-04T	1988:2001	strain YLB-04T	1988:2001	In addition to diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), the polar lipids of strain YLB-02T also consisted of an unidentified glycolipid (GL), two unidentified polar lipids (L1 and L2) and two unidentified phospholipids (PL1 and PL2), and those of strain YLB-04T also consisted of phosphatidylethanolamine (PE) and an unidentified phospholipid (PL).
31364965	16	53	theme	type	2352:2355	arg1	strains					2357:2363	the type strains	2348:2363	the type strains	2348:2363	nov. with YLB-04T (=MCCC 1A12711T=JCM 32872T) as the type strains.
31364965	0	54	dep	species	10:16	arg1	sp					74:75	Oceanobacillus piezotolerans sp	45:75	Two novel species of the family Bacillaceae: Oceanobacillus piezotolerans sp.	0:76	Two novel species of the family Bacillaceae: Oceanobacillus piezotolerans sp.
31364965	8	55	theme	DNA-DNA	956:962	arg1	DDH					979:981	DDH	979:981	DDH	979:981	The average nucleotide identity (ANI) and the DNA-DNA hybridisation (DDH) estimate values for strain YLB-02T and YLB-04T with their related type strains were below the respective threshold for species differentiation.
31364965	8	55	theme	DNA-DNA	956:962	arg1	hybridisation					964:976	the DNA-DNA hybridisation	952:976	the DNA-DNA hybridisation (DDH)	952:982	The average nucleotide identity (ANI) and the DNA-DNA hybridisation (DDH) estimate values for strain YLB-02T and YLB-04T with their related type strains were below the respective threshold for species differentiation.
31364965	3	56	attach	isolated	222:229	arg1	sediments					249:257	the deep-sea sediments	236:257	the deep-sea sediments of Yap Trench located in the Pacific Ocean	236:300	Two novel strains, designated YLB-02T and YLB-04T, were isolated from the deep-sea sediments of Yap Trench located in the Pacific Ocean.
31364965	3	56	attach	isolated	222:229	arg2	strains					176:182	Two novel strains	166:182	Two novel strains	166:182	Two novel strains, designated YLB-02T and YLB-04T, were isolated from the deep-sea sediments of Yap Trench located in the Pacific Ocean.
31364965	6	57	theme	Oceanobacillus	722:735	arg1	J8BT					748:751	Oceanobacillus halophilus J8BT	722:751	Oceanobacillus halophilus J8BT	722:751	Strain YLB-02T showed similarities of 96.9 % with Ornithinibacillus contaminans CCUG 53201T, 96.3 % with Oceanobacillus profundus CL-MP28T, 96.1 % with Oceanobacillus halophilus J8BT and 95.7 % with Oceanobacillus bengalensis Ma-21T.
31364965	5	58	theme	gene	437:440	arg1	sequences					442:450	16S rRNA gene sequences	428:450	16S rRNA gene sequences	428:450	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain YLB-02T belonged to the genus Oceanobacillus and strain YLB-04T belonged to the genus Bacillus.
31364965	3	59	from	located	273:279	arg1	Ocean					296:300	the Pacific Ocean	284:300	the Pacific Ocean	284:300	Two novel strains, designated YLB-02T and YLB-04T, were isolated from the deep-sea sediments of Yap Trench located in the Pacific Ocean.
31364965	6	60	with	%	762:762	arg1	CL-MP28T					700:707	Oceanobacillus profundus CL-MP28T	675:707	Oceanobacillus profundus CL-MP28T	675:707	Strain YLB-02T showed similarities of 96.9 % with Ornithinibacillus contaminans CCUG 53201T, 96.3 % with Oceanobacillus profundus CL-MP28T, 96.1 % with Oceanobacillus halophilus J8BT and 95.7 % with Oceanobacillus bengalensis Ma-21T.
31364965	6	60	with	%	762:762	arg1	Ma-21T					796:801	Oceanobacillus bengalensis Ma-21T	769:801	Oceanobacillus bengalensis Ma-21T	769:801	Strain YLB-02T showed similarities of 96.9 % with Ornithinibacillus contaminans CCUG 53201T, 96.3 % with Oceanobacillus profundus CL-MP28T, 96.1 % with Oceanobacillus halophilus J8BT and 95.7 % with Oceanobacillus bengalensis Ma-21T.
31364965	6	60	with	%	762:762	arg1	53201T					655:660	Ornithinibacillus contaminans CCUG 53201T	620:660	Ornithinibacillus contaminans CCUG 53201T	620:660	Strain YLB-02T showed similarities of 96.9 % with Ornithinibacillus contaminans CCUG 53201T, 96.3 % with Oceanobacillus profundus CL-MP28T, 96.1 % with Oceanobacillus halophilus J8BT and 95.7 % with Oceanobacillus bengalensis Ma-21T.
31364965	6	60	with	%	762:762	arg1	J8BT					748:751	Oceanobacillus halophilus J8BT	722:751	Oceanobacillus halophilus J8BT	722:751	Strain YLB-02T showed similarities of 96.9 % with Ornithinibacillus contaminans CCUG 53201T, 96.3 % with Oceanobacillus profundus CL-MP28T, 96.1 % with Oceanobacillus halophilus J8BT and 95.7 % with Oceanobacillus bengalensis Ma-21T.
31364965	6	61	theme	%	715:715	arg1	similarities					592:603	similarities	592:603	similarities of 96.9 % with Ornithinibacillus contaminans CCUG 53201T, 96.3 % with Oceanobacillus profundus CL-MP28T, 96.1 % with Oceanobacillus halophilus J8BT and 95.7 % with Oceanobacillus bengalensis Ma-21T	592:801	Strain YLB-02T showed similarities of 96.9 % with Ornithinibacillus contaminans CCUG 53201T, 96.3 % with Oceanobacillus profundus CL-MP28T, 96.1 % with Oceanobacillus halophilus J8BT and 95.7 % with Oceanobacillus bengalensis Ma-21T.
31364965	5	62	theme	Phylogenetic	397:408	arg1	analyses					410:417	Phylogenetic analyses	397:417	Phylogenetic analyses based on 16S rRNA gene sequences	397:450	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain YLB-02T belonged to the genus Oceanobacillus and strain YLB-04T belonged to the genus Bacillus.
31364965	12	63	theme	glutamic	1661:1668	arg1	acid					1670:1673	glutamic acid	1661:1673	glutamic acid	1661:1673	The cell-wall peptidoglycan of strain YLB-02T contained glutamic acid, alanine, aspartic acid, lysine and ornithine, but no meso-diaminopimelic acid, while strain YLB-04T contained meso-diaminopimelic acid, glutamic acid, alanine, aspartic acid, lysine and ornithine.
31364965	16	64	theme	1A12711T=JCM	2324:2335	arg1	32872T					2337:2342	=MCCC 1A12711T=JCM 32872T	2318:2342	=MCCC 1A12711T=JCM 32872T	2318:2342	nov. with YLB-04T (=MCCC 1A12711T=JCM 32872T) as the type strains.
31364965	16	64	theme	1A12711T=JCM	2324:2335	arg1	YLB-04T					2309:2315	YLB-04T	2309:2315	YLB-04T (=MCCC 1A12711T=JCM 32872T)	2309:2343	nov. with YLB-04T (=MCCC 1A12711T=JCM 32872T) as the type strains.
31364965	7	65	theme	notoginsengisoli	882:897	arg1	SYP-B691T					899:907	Bacillus notoginsengisoli SYP-B691T	873:907	Bacillus notoginsengisoli SYP-B691T	873:907	Strain YLB-04T showed the highest sequence similarity of 97.4 % with Bacillus notoginsengisoli SYP-B691T.
31364965	10	66	theme	predominant	1205:1215	arg1	iso-C14 					1269:1276	iso-C14 	1269:1276	iso-C14 : 0, iso-C15 : 0, iso-C16 : 0 and C16 : 1ω7c alcohol	1269:1328	The predominant (>10 %) cellular fatty acids of strain YLB-02T were iso-C14 : 0, iso-C15 : 0, iso-C16 : 0 and C16 : 1ω7c alcohol, and those of strain YLB-04T were C16 : 0, iso-C15 : 0, anteiso-C15 : 0 and C18 : 0.
31364965	10	66	theme	predominant	1205:1215	arg1	acids					1240:1244	The predominant (>10 %) cellular fatty acids	1201:1244	The predominant (>10 %) cellular fatty acids of strain YLB-02T	1201:1262	The predominant (>10 %) cellular fatty acids of strain YLB-02T were iso-C14 : 0, iso-C15 : 0, iso-C16 : 0 and C16 : 1ω7c alcohol, and those of strain YLB-04T were C16 : 0, iso-C15 : 0, anteiso-C15 : 0 and C18 : 0.
31364965	6	67	dep	Oceanobacillus	769:782	arg1	bengalensis					784:794	bengalensis	784:794	bengalensis	784:794	Strain YLB-02T showed similarities of 96.9 % with Ornithinibacillus contaminans CCUG 53201T, 96.3 % with Oceanobacillus profundus CL-MP28T, 96.1 % with Oceanobacillus halophilus J8BT and 95.7 % with Oceanobacillus bengalensis Ma-21T.
31364965	2	68	theme	sediment	134:141	arg1	samples					143:149	deep-sea sediment samples	125:149	deep-sea sediment samples of Yap Trench	125:163	nov., from deep-sea sediment samples of Yap Trench.
31364965	11	69	theme	predominant	1421:1431	arg1	ubiquinone					1433:1442	Their predominant ubiquinone	1415:1442	Their predominant ubiquinone	1415:1442	Their predominant ubiquinone was MK-7.
31364965	8	70	theme	strain	1004:1009	arg1	YLB-02T					1011:1017	strain YLB-02T	1004:1017	strain YLB-02T	1004:1017	The average nucleotide identity (ANI) and the DNA-DNA hybridisation (DDH) estimate values for strain YLB-02T and YLB-04T with their related type strains were below the respective threshold for species differentiation.
31364965	12	71	theme	glutamic	1510:1517	arg1	acid					1519:1522	glutamic acid	1510:1522	glutamic acid	1510:1522	The cell-wall peptidoglycan of strain YLB-02T contained glutamic acid, alanine, aspartic acid, lysine and ornithine, but no meso-diaminopimelic acid, while strain YLB-04T contained meso-diaminopimelic acid, glutamic acid, alanine, aspartic acid, lysine and ornithine.
31364965	10	72	theme	strain	1344:1349	arg1	YLB-04T					1351:1357	strain YLB-04T	1344:1357	strain YLB-04T	1344:1357	The predominant (>10 %) cellular fatty acids of strain YLB-02T were iso-C14 : 0, iso-C15 : 0, iso-C16 : 0 and C16 : 1ω7c alcohol, and those of strain YLB-04T were C16 : 0, iso-C15 : 0, anteiso-C15 : 0 and C18 : 0.
31364965	13	73	theme	YLB-02T	1824:1830	arg1	lipids					1807:1812	the polar lipids	1797:1812	the polar lipids of strain YLB-02T	1797:1830	In addition to diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), the polar lipids of strain YLB-02T also consisted of an unidentified glycolipid (GL), two unidentified polar lipids (L1 and L2) and two unidentified phospholipids (PL1 and PL2), and those of strain YLB-04T also consisted of phosphatidylethanolamine (PE) and an unidentified phospholipid (PL).
31364965	6	74	theme	Strain	570:575	arg1	YLB-02T					577:583	Strain YLB-02T	570:583	Strain YLB-02T	570:583	Strain YLB-02T showed similarities of 96.9 % with Ornithinibacillus contaminans CCUG 53201T, 96.3 % with Oceanobacillus profundus CL-MP28T, 96.1 % with Oceanobacillus halophilus J8BT and 95.7 % with Oceanobacillus bengalensis Ma-21T.
31364965	14	75	theme	genotypic	2111:2119	arg1	characteristics					2140:2154	phenotypic, genotypic and chemotaxonomic characteristics	2099:2154	phenotypic, genotypic and chemotaxonomic characteristics	2099:2154	Based on phenotypic, genotypic and chemotaxonomic characteristics, two novel species are proposed, Oceanobacillus piezotolerans sp.
31364965	7	76	theme	highest	830:836	arg1	similarity					847:856	the highest sequence similarity	826:856	the highest sequence similarity of 97.4 % with Bacillus notoginsengisoli SYP-B691T	826:907	Strain YLB-04T showed the highest sequence similarity of 97.4 % with Bacillus notoginsengisoli SYP-B691T.
31364965	15	77	theme	Bacillus	2272:2279	arg1	sp					2295:2296	Bacillus piezotolerans sp	2272:2296	Bacillus piezotolerans sp	2272:2296	nov. with YLB-02T (=MCCC 1A12699T=JCM 32870T) and Bacillus piezotolerans sp.
31364965	15	78	dep	nov.	2222:2225	arg1	YLB-02T					2232:2238	YLB-02T	2232:2238	YLB-02T (=MCCC 1A12699T=JCM 32870T)	2232:2266	nov. with YLB-02T (=MCCC 1A12699T=JCM 32870T) and Bacillus piezotolerans sp.
31364965	15	78	dep	nov.	2222:2225	arg1	sp					2295:2296	Bacillus piezotolerans sp	2272:2296	Bacillus piezotolerans sp	2272:2296	nov. with YLB-02T (=MCCC 1A12699T=JCM 32870T) and Bacillus piezotolerans sp.
31364965	15	78	dep	nov.	2222:2225	arg1	32870T					2260:2265	=MCCC 1A12699T=JCM 32870T	2241:2265	=MCCC 1A12699T=JCM 32870T	2241:2265	nov. with YLB-02T (=MCCC 1A12699T=JCM 32870T) and Bacillus piezotolerans sp.
31364965	3	79	theme	deep-sea	240:247	arg1	sediments					249:257	the deep-sea sediments	236:257	the deep-sea sediments of Yap Trench located in the Pacific Ocean	236:300	Two novel strains, designated YLB-02T and YLB-04T, were isolated from the deep-sea sediments of Yap Trench located in the Pacific Ocean.
31364965	12	80	theme	YLB-02T	1492:1498	arg1	peptidoglycan					1468:1480	The cell-wall peptidoglycan	1454:1480	The cell-wall peptidoglycan of strain YLB-02T	1454:1498	The cell-wall peptidoglycan of strain YLB-02T contained glutamic acid, alanine, aspartic acid, lysine and ornithine, but no meso-diaminopimelic acid, while strain YLB-04T contained meso-diaminopimelic acid, glutamic acid, alanine, aspartic acid, lysine and ornithine.
31364965	10	81	theme	fatty	1234:1238	arg1	iso-C14 					1269:1276	iso-C14 	1269:1276	iso-C14 : 0, iso-C15 : 0, iso-C16 : 0 and C16 : 1ω7c alcohol	1269:1328	The predominant (>10 %) cellular fatty acids of strain YLB-02T were iso-C14 : 0, iso-C15 : 0, iso-C16 : 0 and C16 : 1ω7c alcohol, and those of strain YLB-04T were C16 : 0, iso-C15 : 0, anteiso-C15 : 0 and C18 : 0.
31364965	10	81	theme	fatty	1234:1238	arg1	acids					1240:1244	The predominant (>10 %) cellular fatty acids	1201:1244	The predominant (>10 %) cellular fatty acids of strain YLB-02T	1201:1262	The predominant (>10 %) cellular fatty acids of strain YLB-02T were iso-C14 : 0, iso-C15 : 0, iso-C16 : 0 and C16 : 1ω7c alcohol, and those of strain YLB-04T were C16 : 0, iso-C15 : 0, anteiso-C15 : 0 and C18 : 0.
31364965	13	82	theme	unidentified	1933:1944	arg1	phospholipids					1946:1958	two unidentified phospholipids	1929:1958	two unidentified phospholipids (PL1 and PL2)	1929:1972	In addition to diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), the polar lipids of strain YLB-02T also consisted of an unidentified glycolipid (GL), two unidentified polar lipids (L1 and L2) and two unidentified phospholipids (PL1 and PL2), and those of strain YLB-04T also consisted of phosphatidylethanolamine (PE) and an unidentified phospholipid (PL).
31364965	13	82	theme	unidentified	1933:1944	arg1	PL2					1969:1971	PL2	1969:1971	PL2	1969:1971	In addition to diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), the polar lipids of strain YLB-02T also consisted of an unidentified glycolipid (GL), two unidentified polar lipids (L1 and L2) and two unidentified phospholipids (PL1 and PL2), and those of strain YLB-04T also consisted of phosphatidylethanolamine (PE) and an unidentified phospholipid (PL).
31364965	13	82	theme	unidentified	1933:1944	arg1	PL1					1961:1963	PL1	1961:1963	PL1	1961:1963	In addition to diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), the polar lipids of strain YLB-02T also consisted of an unidentified glycolipid (GL), two unidentified polar lipids (L1 and L2) and two unidentified phospholipids (PL1 and PL2), and those of strain YLB-04T also consisted of phosphatidylethanolamine (PE) and an unidentified phospholipid (PL).
31364965	3	83	theme	Trench	266:271	arg1	sediments					249:257	the deep-sea sediments	236:257	the deep-sea sediments of Yap Trench located in the Pacific Ocean	236:300	Two novel strains, designated YLB-02T and YLB-04T, were isolated from the deep-sea sediments of Yap Trench located in the Pacific Ocean.
31364965	6	84	theme	CCUG	650:653	arg1	53201T					655:660	Ornithinibacillus contaminans CCUG 53201T	620:660	Ornithinibacillus contaminans CCUG 53201T	620:660	Strain YLB-02T showed similarities of 96.9 % with Ornithinibacillus contaminans CCUG 53201T, 96.3 % with Oceanobacillus profundus CL-MP28T, 96.1 % with Oceanobacillus halophilus J8BT and 95.7 % with Oceanobacillus bengalensis Ma-21T.
31364965	0	85	theme	piezotolerans	60:72	arg1	sp					74:75	Oceanobacillus piezotolerans sp	45:75	Two novel species of the family Bacillaceae: Oceanobacillus piezotolerans sp.	0:76	Two novel species of the family Bacillaceae: Oceanobacillus piezotolerans sp.
31364965	14	86	dep	sp	2218:2219	arg1	Oceanobacillus					2189:2202	Oceanobacillus	2189:2202	Oceanobacillus	2189:2202	Based on phenotypic, genotypic and chemotaxonomic characteristics, two novel species are proposed, Oceanobacillus piezotolerans sp.
31364965	12	87	theme	cell-wall	1458:1466	arg1	peptidoglycan					1468:1480	The cell-wall peptidoglycan	1454:1480	The cell-wall peptidoglycan of strain YLB-02T	1454:1498	The cell-wall peptidoglycan of strain YLB-02T contained glutamic acid, alanine, aspartic acid, lysine and ornithine, but no meso-diaminopimelic acid, while strain YLB-04T contained meso-diaminopimelic acid, glutamic acid, alanine, aspartic acid, lysine and ornithine.
31364965	15	88	theme	piezotolerans	2281:2293	arg1	sp					2295:2296	Bacillus piezotolerans sp	2272:2296	Bacillus piezotolerans sp	2272:2296	nov. with YLB-02T (=MCCC 1A12699T=JCM 32870T) and Bacillus piezotolerans sp.
31364965	10	89	theme	YLB-02T	1256:1262	arg1	iso-C14 					1269:1276	iso-C14 	1269:1276	iso-C14 : 0, iso-C15 : 0, iso-C16 : 0 and C16 : 1ω7c alcohol	1269:1328	The predominant (>10 %) cellular fatty acids of strain YLB-02T were iso-C14 : 0, iso-C15 : 0, iso-C16 : 0 and C16 : 1ω7c alcohol, and those of strain YLB-04T were C16 : 0, iso-C15 : 0, anteiso-C15 : 0 and C18 : 0.
31364965	10	89	theme	YLB-02T	1256:1262	arg1	acids					1240:1244	The predominant (>10 %) cellular fatty acids	1201:1244	The predominant (>10 %) cellular fatty acids of strain YLB-02T	1201:1262	The predominant (>10 %) cellular fatty acids of strain YLB-02T were iso-C14 : 0, iso-C15 : 0, iso-C16 : 0 and C16 : 1ω7c alcohol, and those of strain YLB-04T were C16 : 0, iso-C15 : 0, anteiso-C15 : 0 and C18 : 0.
31364965	12	90	contain	contained	1625:1633	arg2	ornithine					1711:1719	ornithine	1711:1719	ornithine	1711:1719	The cell-wall peptidoglycan of strain YLB-02T contained glutamic acid, alanine, aspartic acid, lysine and ornithine, but no meso-diaminopimelic acid, while strain YLB-04T contained meso-diaminopimelic acid, glutamic acid, alanine, aspartic acid, lysine and ornithine.
31364965	12	90	contain	contained	1625:1633	arg2	acid					1655:1658	meso-diaminopimelic acid	1635:1658	meso-diaminopimelic acid	1635:1658	The cell-wall peptidoglycan of strain YLB-02T contained glutamic acid, alanine, aspartic acid, lysine and ornithine, but no meso-diaminopimelic acid, while strain YLB-04T contained meso-diaminopimelic acid, glutamic acid, alanine, aspartic acid, lysine and ornithine.
31364965	12	90	contain	contained	1625:1633	arg2	alanine					1676:1682	alanine	1676:1682	alanine	1676:1682	The cell-wall peptidoglycan of strain YLB-02T contained glutamic acid, alanine, aspartic acid, lysine and ornithine, but no meso-diaminopimelic acid, while strain YLB-04T contained meso-diaminopimelic acid, glutamic acid, alanine, aspartic acid, lysine and ornithine.
31364965	12	90	contain	contained	1625:1633	arg1	YLB-04T					1617:1623	strain YLB-04T	1610:1623	strain YLB-04T	1610:1623	The cell-wall peptidoglycan of strain YLB-02T contained glutamic acid, alanine, aspartic acid, lysine and ornithine, but no meso-diaminopimelic acid, while strain YLB-04T contained meso-diaminopimelic acid, glutamic acid, alanine, aspartic acid, lysine and ornithine.
31364965	12	90	contain	contained	1625:1633	arg2	acid					1694:1697	aspartic acid	1685:1697	aspartic acid	1685:1697	The cell-wall peptidoglycan of strain YLB-02T contained glutamic acid, alanine, aspartic acid, lysine and ornithine, but no meso-diaminopimelic acid, while strain YLB-04T contained meso-diaminopimelic acid, glutamic acid, alanine, aspartic acid, lysine and ornithine.
31364965	12	90	contain	contained	1625:1633	arg2	lysine					1700:1705	lysine	1700:1705	lysine	1700:1705	The cell-wall peptidoglycan of strain YLB-02T contained glutamic acid, alanine, aspartic acid, lysine and ornithine, but no meso-diaminopimelic acid, while strain YLB-04T contained meso-diaminopimelic acid, glutamic acid, alanine, aspartic acid, lysine and ornithine.
31364965	12	90	contain	contained	1625:1633	arg2	acid					1670:1673	glutamic acid	1661:1673	glutamic acid	1661:1673	The cell-wall peptidoglycan of strain YLB-02T contained glutamic acid, alanine, aspartic acid, lysine and ornithine, but no meso-diaminopimelic acid, while strain YLB-04T contained meso-diaminopimelic acid, glutamic acid, alanine, aspartic acid, lysine and ornithine.
31364965	5	91	theme	strain	523:528	arg1	YLB-04T					530:536	strain YLB-04T	523:536	strain YLB-04T	523:536	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain YLB-02T belonged to the genus Oceanobacillus and strain YLB-04T belonged to the genus Bacillus.
31364965	3	92	theme	Pacific	288:294	arg1	Ocean					296:300	the Pacific Ocean	284:300	the Pacific Ocean	284:300	Two novel strains, designated YLB-02T and YLB-04T, were isolated from the deep-sea sediments of Yap Trench located in the Pacific Ocean.
31364965	6	93	theme	%	613:613	arg1	similarities					592:603	similarities	592:603	similarities of 96.9 % with Ornithinibacillus contaminans CCUG 53201T, 96.3 % with Oceanobacillus profundus CL-MP28T, 96.1 % with Oceanobacillus halophilus J8BT and 95.7 % with Oceanobacillus bengalensis Ma-21T	592:801	Strain YLB-02T showed similarities of 96.9 % with Ornithinibacillus contaminans CCUG 53201T, 96.3 % with Oceanobacillus profundus CL-MP28T, 96.1 % with Oceanobacillus halophilus J8BT and 95.7 % with Oceanobacillus bengalensis Ma-21T.
31364965	15	94	theme	1A12699T=JCM	2247:2258	arg1	YLB-02T					2232:2238	YLB-02T	2232:2238	YLB-02T (=MCCC 1A12699T=JCM 32870T)	2232:2266	nov. with YLB-02T (=MCCC 1A12699T=JCM 32870T) and Bacillus piezotolerans sp.
31364965	15	94	theme	1A12699T=JCM	2247:2258	arg1	32870T					2260:2265	=MCCC 1A12699T=JCM 32870T	2241:2265	=MCCC 1A12699T=JCM 32870T	2241:2265	nov. with YLB-02T (=MCCC 1A12699T=JCM 32870T) and Bacillus piezotolerans sp.
31364965	8	95	theme	related	1042:1048	arg1	strains					1055:1061	their related type strains	1036:1061	their related type strains	1036:1061	The average nucleotide identity (ANI) and the DNA-DNA hybridisation (DDH) estimate values for strain YLB-02T and YLB-04T with their related type strains were below the respective threshold for species differentiation.
31364965	8	96	with	YLB-02T	1011:1017	arg1	strains					1055:1061	their related type strains	1036:1061	their related type strains	1036:1061	The average nucleotide identity (ANI) and the DNA-DNA hybridisation (DDH) estimate values for strain YLB-02T and YLB-04T with their related type strains were below the respective threshold for species differentiation.
31364965	6	97	dep	Oceanobacillus	675:688	arg1	profundus					690:698	profundus	690:698	profundus	690:698	Strain YLB-02T showed similarities of 96.9 % with Ornithinibacillus contaminans CCUG 53201T, 96.3 % with Oceanobacillus profundus CL-MP28T, 96.1 % with Oceanobacillus halophilus J8BT and 95.7 % with Oceanobacillus bengalensis Ma-21T.
31364965	14	98	theme	phenotypic	2099:2108	arg1	characteristics					2140:2154	phenotypic, genotypic and chemotaxonomic characteristics	2099:2154	phenotypic, genotypic and chemotaxonomic characteristics	2099:2154	Based on phenotypic, genotypic and chemotaxonomic characteristics, two novel species are proposed, Oceanobacillus piezotolerans sp.
31364965	0	99	theme	novel	4:8	arg1	species					10:16	Two novel species	0:16	Two novel species of the family Bacillaceae: Oceanobacillus piezotolerans sp.	0:76	Two novel species of the family Bacillaceae: Oceanobacillus piezotolerans sp.
31364965	6	100	theme	Oceanobacillus	675:688	arg1	CL-MP28T					700:707	Oceanobacillus profundus CL-MP28T	675:707	Oceanobacillus profundus CL-MP28T	675:707	Strain YLB-02T showed similarities of 96.9 % with Ornithinibacillus contaminans CCUG 53201T, 96.3 % with Oceanobacillus profundus CL-MP28T, 96.1 % with Oceanobacillus halophilus J8BT and 95.7 % with Oceanobacillus bengalensis Ma-21T.
31364965	8	101	theme	respective	1078:1087	arg1	threshold					1089:1097	the respective threshold	1074:1097	the respective threshold for species differentiation	1074:1125	The average nucleotide identity (ANI) and the DNA-DNA hybridisation (DDH) estimate values for strain YLB-02T and YLB-04T with their related type strains were below the respective threshold for species differentiation.
31364965	1	102	theme	Bacillus	87:94	arg1	sp					110:111	Bacillus piezotolerans sp	87:111	Bacillus piezotolerans sp	87:111	nov. and Bacillus piezotolerans sp.
31364965	5	103	theme	strain	467:472	arg1	YLB-02T					474:480	strain YLB-02T	467:480	strain YLB-02T	467:480	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain YLB-02T belonged to the genus Oceanobacillus and strain YLB-04T belonged to the genus Bacillus.
31364965	6	104	theme	%	668:668	arg1	similarities					592:603	similarities	592:603	similarities of 96.9 % with Ornithinibacillus contaminans CCUG 53201T, 96.3 % with Oceanobacillus profundus CL-MP28T, 96.1 % with Oceanobacillus halophilus J8BT and 95.7 % with Oceanobacillus bengalensis Ma-21T	592:801	Strain YLB-02T showed similarities of 96.9 % with Ornithinibacillus contaminans CCUG 53201T, 96.3 % with Oceanobacillus profundus CL-MP28T, 96.1 % with Oceanobacillus halophilus J8BT and 95.7 % with Oceanobacillus bengalensis Ma-21T.
31364965	0	105	theme	family	25:30	arg1	Bacillaceae					32:42	the family Bacillaceae	21:42	the family Bacillaceae	21:42	Two novel species of the family Bacillaceae: Oceanobacillus piezotolerans sp.
31364965	7	106	theme	Strain	804:809	arg1	YLB-04T					811:817	Strain YLB-04T	804:817	Strain YLB-04T	804:817	Strain YLB-04T showed the highest sequence similarity of 97.4 % with Bacillus notoginsengisoli SYP-B691T.
31364965	8	107	theme	nucleotide	922:931	arg1	ANI					943:945	ANI	943:945	ANI	943:945	The average nucleotide identity (ANI) and the DNA-DNA hybridisation (DDH) estimate values for strain YLB-02T and YLB-04T with their related type strains were below the respective threshold for species differentiation.
31364965	8	107	theme	nucleotide	922:931	arg1	identity					933:940	The average nucleotide identity	910:940	The average nucleotide identity (ANI)	910:946	The average nucleotide identity (ANI) and the DNA-DNA hybridisation (DDH) estimate values for strain YLB-02T and YLB-04T with their related type strains were below the respective threshold for species differentiation.
31364965	2	108	theme	Yap	154:156	arg1	Trench					158:163	Yap Trench	154:163	Yap Trench	154:163	nov., from deep-sea sediment samples of Yap Trench.
31364965	6	109	theme	%	762:762	arg1	similarities					592:603	similarities	592:603	similarities of 96.9 % with Ornithinibacillus contaminans CCUG 53201T, 96.3 % with Oceanobacillus profundus CL-MP28T, 96.1 % with Oceanobacillus halophilus J8BT and 95.7 % with Oceanobacillus bengalensis Ma-21T	592:801	Strain YLB-02T showed similarities of 96.9 % with Ornithinibacillus contaminans CCUG 53201T, 96.3 % with Oceanobacillus profundus CL-MP28T, 96.1 % with Oceanobacillus halophilus J8BT and 95.7 % with Oceanobacillus bengalensis Ma-21T.
31364965	7	110	with	similarity	847:856	arg1	SYP-B691T					899:907	Bacillus notoginsengisoli SYP-B691T	873:907	Bacillus notoginsengisoli SYP-B691T	873:907	Strain YLB-04T showed the highest sequence similarity of 97.4 % with Bacillus notoginsengisoli SYP-B691T.
31364965	9	111	theme	strains	1148:1154	arg1	%					1198:1198	37.3 and 45.4 mol%	1181:1198	37.3 and 45.4 mol%	1181:1198	The G+C contents of strains YLB-02T and YLB-04T were 37.3 and 45.4 mol%.
31364965	9	111	theme	strains	1148:1154	arg1	G+C contents					1132:1143	The G+C contents	1128:1143	The G+C contents of strains YLB-02T and YLB-04T	1128:1174	The G+C contents of strains YLB-02T and YLB-04T were 37.3 and 45.4 mol%.
31364965	10	112	theme	 1ω7c	1316:1320	arg1	alcohol					1322:1328	 1ω7c alcohol	1316:1328	iso-C14 : 0, iso-C15 : 0, iso-C16 : 0 and C16 : 1ω7c alcohol	1269:1328	The predominant (>10 %) cellular fatty acids of strain YLB-02T were iso-C14 : 0, iso-C15 : 0, iso-C16 : 0 and C16 : 1ω7c alcohol, and those of strain YLB-04T were C16 : 0, iso-C15 : 0, anteiso-C15 : 0 and C18 : 0.
31364965	5	113	theme	rRNA	432:435	arg1	sequences					442:450	16S rRNA gene sequences	428:450	16S rRNA gene sequences	428:450	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain YLB-02T belonged to the genus Oceanobacillus and strain YLB-04T belonged to the genus Bacillus.
31364965	6	114	dep	Oceanobacillus	722:735	arg1	halophilus					737:746	halophilus	737:746	halophilus	737:746	Strain YLB-02T showed similarities of 96.9 % with Ornithinibacillus contaminans CCUG 53201T, 96.3 % with Oceanobacillus profundus CL-MP28T, 96.1 % with Oceanobacillus halophilus J8BT and 95.7 % with Oceanobacillus bengalensis Ma-21T.
31364965	12	115	contain	contained	1500:1508	arg2	alanine					1525:1531	alanine	1525:1531	alanine	1525:1531	The cell-wall peptidoglycan of strain YLB-02T contained glutamic acid, alanine, aspartic acid, lysine and ornithine, but no meso-diaminopimelic acid, while strain YLB-04T contained meso-diaminopimelic acid, glutamic acid, alanine, aspartic acid, lysine and ornithine.
31364965	12	115	contain	contained	1500:1508	arg1	peptidoglycan					1468:1480	The cell-wall peptidoglycan	1454:1480	The cell-wall peptidoglycan of strain YLB-02T	1454:1498	The cell-wall peptidoglycan of strain YLB-02T contained glutamic acid, alanine, aspartic acid, lysine and ornithine, but no meso-diaminopimelic acid, while strain YLB-04T contained meso-diaminopimelic acid, glutamic acid, alanine, aspartic acid, lysine and ornithine.
31364965	12	115	contain	contained	1500:1508	arg2	acid					1519:1522	glutamic acid	1510:1522	glutamic acid	1510:1522	The cell-wall peptidoglycan of strain YLB-02T contained glutamic acid, alanine, aspartic acid, lysine and ornithine, but no meso-diaminopimelic acid, while strain YLB-04T contained meso-diaminopimelic acid, glutamic acid, alanine, aspartic acid, lysine and ornithine.
31364965	12	115	contain	contained	1500:1508	arg2	acid					1598:1601	no meso-diaminopimelic acid	1575:1601	no meso-diaminopimelic acid	1575:1601	The cell-wall peptidoglycan of strain YLB-02T contained glutamic acid, alanine, aspartic acid, lysine and ornithine, but no meso-diaminopimelic acid, while strain YLB-04T contained meso-diaminopimelic acid, glutamic acid, alanine, aspartic acid, lysine and ornithine.
31733862	6	0	theme	lactose	862:868	arg1	synthesis					870:878	lactose synthesis	862:878	lactose synthesis	862:878	We quantified the expression of genes involved in lipid and lactose synthesis using real-time quantitative PCR.
31733862	9	1	dep	A-769662	1194:1201	arg1	A76					1204:1206	A76	1204:1206	A76; an allosteric activator of AMPK	1204:1239	To assess the effect of AMPK activation on milk component synthesis, we treated BMEC with 100 μM A-769662 (A76; an allosteric activator of AMPK) or vehicle control for 16 h. Consistent with activation of AMPK, A76 increased phosphorylation of its downstream targets ACC Ser79 and TSC2 Ser1387 by 144% and 26%, respectively.
31733862	13	2	theme	SLC2A1	1690:1695	arg1	expression					1676:1685	the expression	1672:1685	the expression of SLC2A1, the gene encoding GLUT1	1672:1720	In addition, AMPK activation reduced lactose synthesis by 24% and lowered the expression of SLC2A1, the gene encoding GLUT1.
31733862	11	3	theme	increased	1505:1513	arg1	expression					1515:1524	increased expression	1505:1524	increased expression of FABP3	1505:1533	This effect was accompanied by increased expression of FABP3.
31733862	9	4	with	Consistent	1271:1280	arg1	activation					1287:1296	activation	1287:1296	activation of AMPK	1287:1304	To assess the effect of AMPK activation on milk component synthesis, we treated BMEC with 100 μM A-769662 (A76; an allosteric activator of AMPK) or vehicle control for 16 h. Consistent with activation of AMPK, A76 increased phosphorylation of its downstream targets ACC Ser79 and TSC2 Ser1387 by 144% and 26%, respectively.
31733862	16	5	theme	de	2226:2227	arg1	synthesis					2252:2260	de novo lipid and lactose synthesis	2226:2260	de novo lipid and lactose synthesis in BMEC	2226:2268	We concluded that activation of AMPK inhibits de novo lipid and lactose synthesis in BMEC.
31733862	14	6	dep	h.	1923:1924	arg1	promoted					1994:2001	promoted	1994:2001	promoted AMPKα phosphorylation at Thr172 by 84% or 58%, respectively	1994:2061	To assess the regulation of AMPK by energy substrate availability, we incubated BMEC in a control medium containing 4 mM D-glucose and 1 mM sodium acetate, or medium lacking glucose or acetate, for 4 h. Compared with the control medium, deprivation of glucose or acetate promoted AMPKα phosphorylation at Thr172 by 84% or 58%, respectively.
31733862	14	7	contain	containing	1828:1837	arg2	acetate					1870:1876	1 mM sodium acetate	1858:1876	1 mM sodium acetate	1858:1876	To assess the regulation of AMPK by energy substrate availability, we incubated BMEC in a control medium containing 4 mM D-glucose and 1 mM sodium acetate, or medium lacking glucose or acetate, for 4 h. Compared with the control medium, deprivation of glucose or acetate promoted AMPKα phosphorylation at Thr172 by 84% or 58%, respectively.
31733862	14	7	contain	containing	1828:1837	arg2	D-glucose					1844:1852	4 mM D-glucose	1839:1852	4 mM D-glucose	1839:1852	To assess the regulation of AMPK by energy substrate availability, we incubated BMEC in a control medium containing 4 mM D-glucose and 1 mM sodium acetate, or medium lacking glucose or acetate, for 4 h. Compared with the control medium, deprivation of glucose or acetate promoted AMPKα phosphorylation at Thr172 by 84% or 58%, respectively.
31733862	14	7	contain	containing	1828:1837	arg1	medium					1821:1826	a control medium	1811:1826	a control medium containing 4 mM D-glucose and 1 mM sodium acetate	1811:1876	To assess the regulation of AMPK by energy substrate availability, we incubated BMEC in a control medium containing 4 mM D-glucose and 1 mM sodium acetate, or medium lacking glucose or acetate, for 4 h. Compared with the control medium, deprivation of glucose or acetate promoted AMPKα phosphorylation at Thr172 by 84% or 58%, respectively.
31733862	9	8	theme	activation	1126:1135	arg1	effect					1111:1116	the effect	1107:1116	the effect of AMPK activation on milk component synthesis	1107:1163	To assess the effect of AMPK activation on milk component synthesis, we treated BMEC with 100 μM A-769662 (A76; an allosteric activator of AMPK) or vehicle control for 16 h. Consistent with activation of AMPK, A76 increased phosphorylation of its downstream targets ACC Ser79 and TSC2 Ser1387 by 144% and 26%, respectively.
31733862	16	9	theme	lipid	2234:2238	arg1	synthesis					2252:2260	de novo lipid and lactose synthesis	2226:2260	de novo lipid and lactose synthesis in BMEC	2226:2268	We concluded that activation of AMPK inhibits de novo lipid and lactose synthesis in BMEC.
31733862	4	10	theme	mammary	592:598	arg1	tissue					600:605	the mammary tissue	588:605	the mammary tissue of 3 lactating Holstein cows	588:634	We isolated primary BMEC from the mammary tissue of 3 lactating Holstein cows and differentiated them with lactogenic hormones for 4 d.
31733862	14	11	theme	AMPKα	2003:2007	arg1	phosphorylation					2009:2023	AMPKα phosphorylation	2003:2023	AMPKα phosphorylation	2003:2023	To assess the regulation of AMPK by energy substrate availability, we incubated BMEC in a control medium containing 4 mM D-glucose and 1 mM sodium acetate, or medium lacking glucose or acetate, for 4 h. Compared with the control medium, deprivation of glucose or acetate promoted AMPKα phosphorylation at Thr172 by 84% or 58%, respectively.
31733862	9	12	theme	milk	1140:1143	arg1	synthesis					1155:1163	milk component synthesis	1140:1163	milk component synthesis	1140:1163	To assess the effect of AMPK activation on milk component synthesis, we treated BMEC with 100 μM A-769662 (A76; an allosteric activator of AMPK) or vehicle control for 16 h. Consistent with activation of AMPK, A76 increased phosphorylation of its downstream targets ACC Ser79 and TSC2 Ser1387 by 144% and 26%, respectively.
31733862	9	13	theme	downstream	1344:1353	arg1	targets					1355:1361	its downstream targets	1340:1361	its downstream targets ACC Ser79 and TSC2 Ser1387	1340:1388	To assess the effect of AMPK activation on milk component synthesis, we treated BMEC with 100 μM A-769662 (A76; an allosteric activator of AMPK) or vehicle control for 16 h. Consistent with activation of AMPK, A76 increased phosphorylation of its downstream targets ACC Ser79 and TSC2 Ser1387 by 144% and 26%, respectively.
31733862	9	13	theme	downstream	1344:1353	arg1	Ser79					1367:1371	ACC Ser79	1363:1371	ACC Ser79	1363:1371	To assess the effect of AMPK activation on milk component synthesis, we treated BMEC with 100 μM A-769662 (A76; an allosteric activator of AMPK) or vehicle control for 16 h. Consistent with activation of AMPK, A76 increased phosphorylation of its downstream targets ACC Ser79 and TSC2 Ser1387 by 144% and 26%, respectively.
31733862	9	13	theme	downstream	1344:1353	arg1	Ser1387					1382:1388	TSC2 Ser1387	1377:1388	TSC2 Ser1387	1377:1388	To assess the effect of AMPK activation on milk component synthesis, we treated BMEC with 100 μM A-769662 (A76; an allosteric activator of AMPK) or vehicle control for 16 h. Consistent with activation of AMPK, A76 increased phosphorylation of its downstream targets ACC Ser79 and TSC2 Ser1387 by 144% and 26%, respectively.
31733862	16	14	theme	lactose	2244:2250	arg1	synthesis					2252:2260	de novo lipid and lactose synthesis	2226:2260	de novo lipid and lactose synthesis in BMEC	2226:2268	We concluded that activation of AMPK inhibits de novo lipid and lactose synthesis in BMEC.
31733862	13	15	theme	lactose	1635:1641	arg1	synthesis					1643:1651	lactose synthesis	1635:1651	lactose synthesis	1635:1651	In addition, AMPK activation reduced lactose synthesis by 24% and lowered the expression of SLC2A1, the gene encoding GLUT1.
31733862	9	16	theme	ACC	1363:1365	arg1	targets					1355:1361	its downstream targets	1340:1361	its downstream targets ACC Ser79 and TSC2 Ser1387	1340:1388	To assess the effect of AMPK activation on milk component synthesis, we treated BMEC with 100 μM A-769662 (A76; an allosteric activator of AMPK) or vehicle control for 16 h. Consistent with activation of AMPK, A76 increased phosphorylation of its downstream targets ACC Ser79 and TSC2 Ser1387 by 144% and 26%, respectively.
31733862	9	16	theme	ACC	1363:1365	arg1	Ser79					1367:1371	ACC Ser79	1363:1371	ACC Ser79	1363:1371	To assess the effect of AMPK activation on milk component synthesis, we treated BMEC with 100 μM A-769662 (A76; an allosteric activator of AMPK) or vehicle control for 16 h. Consistent with activation of AMPK, A76 increased phosphorylation of its downstream targets ACC Ser79 and TSC2 Ser1387 by 144% and 26%, respectively.
31733862	4	17	theme	primary	570:576	arg1	BMEC					578:581	primary BMEC	570:581	primary BMEC	570:581	We isolated primary BMEC from the mammary tissue of 3 lactating Holstein cows and differentiated them with lactogenic hormones for 4 d.
31733862	14	18	theme	glucose	1975:1981	arg1	deprivation					1960:1970	deprivation	1960:1970	deprivation of glucose or acetate	1960:1992	To assess the regulation of AMPK by energy substrate availability, we incubated BMEC in a control medium containing 4 mM D-glucose and 1 mM sodium acetate, or medium lacking glucose or acetate, for 4 h. Compared with the control medium, deprivation of glucose or acetate promoted AMPKα phosphorylation at Thr172 by 84% or 58%, respectively.
31733862	2	19	theme	energy	270:275	arg1	gauge					277:281	a cellular energy gauge	259:281	a cellular energy gauge that controls anabolic and catabolic processes to maintain a balance between energy supply and demand	259:383	The AMP-activated protein kinase (AMPK) is a cellular energy gauge that controls anabolic and catabolic processes to maintain a balance between energy supply and demand.
31733862	2	19	theme	energy	270:275	arg1	kinase					242:247	The AMP-activated protein kinase	216:247	The AMP-activated protein kinase (AMPK)	216:254	The AMP-activated protein kinase (AMPK) is a cellular energy gauge that controls anabolic and catabolic processes to maintain a balance between energy supply and demand.
31733862	1	20	theme	adequate	189:196	arg1	supply					198:203	an adequate supply	186:203	an adequate supply of energy	186:213	The synthesis of milk components in bovine mammary epithelial cells (BMEC) requires an adequate supply of energy.
31733862	15	21	theme	glucose	2154:2160	arg1	devoid					2144:2149	devoid	2144:2149	devoid	2144:2149	Activation of AMPK was significantly increased in BMEC only when the medium was devoid of glucose for at least 4 h.
31733862	15	21	theme	glucose	2154:2160	arg1	medium					2133:2138	the medium	2129:2138	the medium	2129:2138	Activation of AMPK was significantly increased in BMEC only when the medium was devoid of glucose for at least 4 h.
31733862	6	22	theme	quantitative	896:907	arg1	PCR					909:911	real-time quantitative PCR	886:911	real-time quantitative PCR	886:911	We quantified the expression of genes involved in lipid and lactose synthesis using real-time quantitative PCR.
31733862	15	23	theme	AMPK	2078:2081	arg1	Activation					2064:2073	Activation	2064:2073	Activation of AMPK	2064:2081	Activation of AMPK was significantly increased in BMEC only when the medium was devoid of glucose for at least 4 h.
31733862	9	24	theme	100	1187:1189	arg1	μM					1191:1192	μM	1191:1192	μM	1191:1192	To assess the effect of AMPK activation on milk component synthesis, we treated BMEC with 100 μM A-769662 (A76; an allosteric activator of AMPK) or vehicle control for 16 h. Consistent with activation of AMPK, A76 increased phosphorylation of its downstream targets ACC Ser79 and TSC2 Ser1387 by 144% and 26%, respectively.
31733862	1	25	theme	milk	119:122	arg1	components					124:133	milk components	119:133	milk components	119:133	The synthesis of milk components in bovine mammary epithelial cells (BMEC) requires an adequate supply of energy.
31733862	12	26	theme	SREBP-1c	1588:1595	arg1	proportion					1567:1576	the proportion	1563:1576	the proportion of mature SREBP-1c	1563:1595	Activation of AMPK reduced the proportion of mature SREBP-1c.
31733862	17	27	theme	component	2445:2453	arg1	synthesis					2455:2463	milk component synthesis	2440:2463	milk component synthesis	2440:2463	Further studies are needed to assess the physiological relevance of AMPK activation for milk composition in vivo and to identify the mechanisms mediating its effects on milk component synthesis.
31733862	7	28	theme	substrates	994:1003	arg1	incorporation					965:977	incorporation	965:977	incorporation of radioactive substrates	965:1003	We measured de novo lipid and lactose synthesis by incorporation of radioactive substrates.
31733862	9	29	dep	increased	1311:1319	arg1	Consistent					1271:1280	Consistent	1271:1280	Consistent	1271:1280	To assess the effect of AMPK activation on milk component synthesis, we treated BMEC with 100 μM A-769662 (A76; an allosteric activator of AMPK) or vehicle control for 16 h. Consistent with activation of AMPK, A76 increased phosphorylation of its downstream targets ACC Ser79 and TSC2 Ser1387 by 144% and 26%, respectively.
31733862	4	30	theme	lactating	612:620	arg1	cows					631:634	3 lactating Holstein cows	610:634	3 lactating Holstein cows	610:634	We isolated primary BMEC from the mammary tissue of 3 lactating Holstein cows and differentiated them with lactogenic hormones for 4 d.
31733862	7	31	dep	de	926:927	arg1	novo					929:932	novo	929:932	novo	929:932	We measured de novo lipid and lactose synthesis by incorporation of radioactive substrates.
31733862	2	32	theme	protein	234:240	arg1	kinase					242:247	The AMP-activated protein kinase	216:247	The AMP-activated protein kinase (AMPK)	216:254	The AMP-activated protein kinase (AMPK) is a cellular energy gauge that controls anabolic and catabolic processes to maintain a balance between energy supply and demand.
31733862	2	32	theme	protein	234:240	arg1	gauge					277:281	a cellular energy gauge	259:281	a cellular energy gauge that controls anabolic and catabolic processes to maintain a balance between energy supply and demand	259:383	The AMP-activated protein kinase (AMPK) is a cellular energy gauge that controls anabolic and catabolic processes to maintain a balance between energy supply and demand.
31733862	2	32	theme	protein	234:240	arg1	AMPK					250:253	AMPK	250:253	AMPK	250:253	The AMP-activated protein kinase (AMPK) is a cellular energy gauge that controls anabolic and catabolic processes to maintain a balance between energy supply and demand.
31733862	14	33	from	BMEC	1803:1806	arg1	medium					1882:1887	medium	1882:1887	medium lacking glucose or acetate	1882:1914	To assess the regulation of AMPK by energy substrate availability, we incubated BMEC in a control medium containing 4 mM D-glucose and 1 mM sodium acetate, or medium lacking glucose or acetate, for 4 h. Compared with the control medium, deprivation of glucose or acetate promoted AMPKα phosphorylation at Thr172 by 84% or 58%, respectively.
31733862	14	33	from	BMEC	1803:1806	arg1	medium					1821:1826	a control medium	1811:1826	a control medium containing 4 mM D-glucose and 1 mM sodium acetate	1811:1876	To assess the regulation of AMPK by energy substrate availability, we incubated BMEC in a control medium containing 4 mM D-glucose and 1 mM sodium acetate, or medium lacking glucose or acetate, for 4 h. Compared with the control medium, deprivation of glucose or acetate promoted AMPKα phosphorylation at Thr172 by 84% or 58%, respectively.
31733862	1	34	theme	mammary	145:151	arg1	BMEC					171:174	BMEC	171:174	BMEC	171:174	The synthesis of milk components in bovine mammary epithelial cells (BMEC) requires an adequate supply of energy.
31733862	1	34	theme	mammary	145:151	arg1	cells					164:168	bovine mammary epithelial cells	138:168	bovine mammary epithelial cells (BMEC)	138:175	The synthesis of milk components in bovine mammary epithelial cells (BMEC) requires an adequate supply of energy.
31733862	3	35	theme	de	450:451	arg1	lipid					458:462	de novo lipid and lactose synthesis	450:484	lipid	458:462	The objectives of this study were to assess the role of AMPK on de novo lipid and lactose synthesis, as well as its regulation by glucose and acetate availability in BMEC.
31733862	14	36	theme	control	1944:1950	arg1	medium					1952:1957	the control medium	1940:1957	the control medium	1940:1957	To assess the regulation of AMPK by energy substrate availability, we incubated BMEC in a control medium containing 4 mM D-glucose and 1 mM sodium acetate, or medium lacking glucose or acetate, for 4 h. Compared with the control medium, deprivation of glucose or acetate promoted AMPKα phosphorylation at Thr172 by 84% or 58%, respectively.
31733862	4	37	theme	cows	631:634	arg1	tissue					600:605	the mammary tissue	588:605	the mammary tissue of 3 lactating Holstein cows	588:634	We isolated primary BMEC from the mammary tissue of 3 lactating Holstein cows and differentiated them with lactogenic hormones for 4 d.
31733862	0	38	theme	mammary	76:82	arg1	cells					95:99	bovine mammary epithelial cells	69:99	bovine mammary epithelial cells	69:99	AMP-activated protein kinase controls lipid and lactose synthesis in bovine mammary epithelial cells.
31733862	17	39	from	effects	2429:2435	arg1	synthesis					2455:2463	milk component synthesis	2440:2463	milk component synthesis	2440:2463	Further studies are needed to assess the physiological relevance of AMPK activation for milk composition in vivo and to identify the mechanisms mediating its effects on milk component synthesis.
31733862	14	40	theme	mM	1860:1861	arg1	acetate					1870:1876	1 mM sodium acetate	1858:1876	1 mM sodium acetate	1858:1876	To assess the regulation of AMPK by energy substrate availability, we incubated BMEC in a control medium containing 4 mM D-glucose and 1 mM sodium acetate, or medium lacking glucose or acetate, for 4 h. Compared with the control medium, deprivation of glucose or acetate promoted AMPKα phosphorylation at Thr172 by 84% or 58%, respectively.
31733862	3	41	theme	study	409:413	arg1	objectives					390:399	The objectives	386:399	The objectives of this study	386:413	The objectives of this study were to assess the role of AMPK on de novo lipid and lactose synthesis, as well as its regulation by glucose and acetate availability in BMEC.
31733862	9	42	theme	AMPK	1236:1239	arg1	activator					1223:1231	an allosteric activator	1209:1231	A76; an allosteric activator of AMPK	1204:1239	To assess the effect of AMPK activation on milk component synthesis, we treated BMEC with 100 μM A-769662 (A76; an allosteric activator of AMPK) or vehicle control for 16 h. Consistent with activation of AMPK, A76 increased phosphorylation of its downstream targets ACC Ser79 and TSC2 Ser1387 by 144% and 26%, respectively.
31733862	16	43	from	synthesis	2252:2260	arg1	BMEC					2265:2268	BMEC	2265:2268	BMEC	2265:2268	We concluded that activation of AMPK inhibits de novo lipid and lactose synthesis in BMEC.
31733862	3	44	dep	de	450:451	arg1	novo					453:456	novo	453:456	novo	453:456	The objectives of this study were to assess the role of AMPK on de novo lipid and lactose synthesis, as well as its regulation by glucose and acetate availability in BMEC.
31733862	12	45	theme	AMPK	1550:1553	arg1	Activation					1536:1545	Activation	1536:1545	Activation of AMPK	1536:1553	Activation of AMPK reduced the proportion of mature SREBP-1c.
31733862	9	46	dep	h.	1268:1269	arg1	increased					1311:1319	increased	1311:1319	increased phosphorylation of its downstream targets ACC Ser79 and TSC2 Ser1387 by 144% and 26%, respectively	1311:1418	To assess the effect of AMPK activation on milk component synthesis, we treated BMEC with 100 μM A-769662 (A76; an allosteric activator of AMPK) or vehicle control for 16 h. Consistent with activation of AMPK, A76 increased phosphorylation of its downstream targets ACC Ser79 and TSC2 Ser1387 by 144% and 26%, respectively.
31733862	17	47	theme	AMPK	2339:2342	arg1	activation					2344:2353	AMPK activation	2339:2353	AMPK activation	2339:2353	Further studies are needed to assess the physiological relevance of AMPK activation for milk composition in vivo and to identify the mechanisms mediating its effects on milk component synthesis.
31733862	2	48	theme	energy	360:365	arg1	supply					367:372	energy supply	360:372	energy supply	360:372	The AMP-activated protein kinase (AMPK) is a cellular energy gauge that controls anabolic and catabolic processes to maintain a balance between energy supply and demand.
31733862	0	49	theme	AMP-activated	0:12	arg1	kinase					22:27	AMP-activated protein kinase	0:27	AMP-activated protein kinase	0:27	AMP-activated protein kinase controls lipid and lactose synthesis in bovine mammary epithelial cells.
31733862	14	50	theme	mM	1841:1842	arg1	D-glucose					1844:1852	4 mM D-glucose	1839:1852	4 mM D-glucose	1839:1852	To assess the regulation of AMPK by energy substrate availability, we incubated BMEC in a control medium containing 4 mM D-glucose and 1 mM sodium acetate, or medium lacking glucose or acetate, for 4 h. Compared with the control medium, deprivation of glucose or acetate promoted AMPKα phosphorylation at Thr172 by 84% or 58%, respectively.
31733862	5	51	theme	proteolytic	760:770	arg1	processing					772:781	proteolytic processing	760:781	proteolytic processing	760:781	We measured protein abundance, site-specific phosphorylation, and proteolytic processing by immunoblotting.
31733862	9	52	dep	targets	1355:1361	arg1	targets					1355:1361	its downstream targets	1340:1361	its downstream targets ACC Ser79 and TSC2 Ser1387	1340:1388	To assess the effect of AMPK activation on milk component synthesis, we treated BMEC with 100 μM A-769662 (A76; an allosteric activator of AMPK) or vehicle control for 16 h. Consistent with activation of AMPK, A76 increased phosphorylation of its downstream targets ACC Ser79 and TSC2 Ser1387 by 144% and 26%, respectively.
31733862	9	52	dep	targets	1355:1361	arg1	Ser79					1367:1371	ACC Ser79	1363:1371	ACC Ser79	1363:1371	To assess the effect of AMPK activation on milk component synthesis, we treated BMEC with 100 μM A-769662 (A76; an allosteric activator of AMPK) or vehicle control for 16 h. Consistent with activation of AMPK, A76 increased phosphorylation of its downstream targets ACC Ser79 and TSC2 Ser1387 by 144% and 26%, respectively.
31733862	9	52	dep	targets	1355:1361	arg1	Ser1387					1382:1388	TSC2 Ser1387	1377:1388	TSC2 Ser1387	1377:1388	To assess the effect of AMPK activation on milk component synthesis, we treated BMEC with 100 μM A-769662 (A76; an allosteric activator of AMPK) or vehicle control for 16 h. Consistent with activation of AMPK, A76 increased phosphorylation of its downstream targets ACC Ser79 and TSC2 Ser1387 by 144% and 26%, respectively.
31733862	0	53	theme	lipid	38:42	arg1	synthesis					56:64	lipid and lactose synthesis	38:64	lipid and lactose synthesis in bovine mammary epithelial cells	38:99	AMP-activated protein kinase controls lipid and lactose synthesis in bovine mammary epithelial cells.
31733862	8	54	theme	randomized	1040:1049	arg1	design					1066:1071	a randomized complete block design	1038:1071	a randomized complete block design	1038:1071	We analyzed data by ANOVA using a randomized complete block design with PROC MIXED in SAS.
31733862	14	55	theme	control	1813:1819	arg1	medium					1821:1826	a control medium	1811:1826	a control medium containing 4 mM D-glucose and 1 mM sodium acetate	1811:1876	To assess the regulation of AMPK by energy substrate availability, we incubated BMEC in a control medium containing 4 mM D-glucose and 1 mM sodium acetate, or medium lacking glucose or acetate, for 4 h. Compared with the control medium, deprivation of glucose or acetate promoted AMPKα phosphorylation at Thr172 by 84% or 58%, respectively.
31733862	5	56	theme	site-specific	725:737	arg1	phosphorylation					739:753	site-specific phosphorylation	725:753	site-specific phosphorylation	725:753	We measured protein abundance, site-specific phosphorylation, and proteolytic processing by immunoblotting.
31733862	8	57	theme	block	1060:1064	arg1	design					1066:1071	a randomized complete block design	1038:1071	a randomized complete block design	1038:1071	We analyzed data by ANOVA using a randomized complete block design with PROC MIXED in SAS.
31733862	3	58	from	role	434:437	arg1	lipid					458:462	de novo lipid and lactose synthesis	450:484	lipid	458:462	The objectives of this study were to assess the role of AMPK on de novo lipid and lactose synthesis, as well as its regulation by glucose and acetate availability in BMEC.
31733862	3	58	from	role	434:437	arg1	synthesis					476:484	de novo lipid and lactose synthesis	450:484	synthesis	476:484	The objectives of this study were to assess the role of AMPK on de novo lipid and lactose synthesis, as well as its regulation by glucose and acetate availability in BMEC.
31733862	1	59	from	synthesis	106:114	arg1	BMEC					171:174	BMEC	171:174	BMEC	171:174	The synthesis of milk components in bovine mammary epithelial cells (BMEC) requires an adequate supply of energy.
31733862	1	59	from	synthesis	106:114	arg1	cells					164:168	bovine mammary epithelial cells	138:168	bovine mammary epithelial cells (BMEC)	138:175	The synthesis of milk components in bovine mammary epithelial cells (BMEC) requires an adequate supply of energy.
31733862	1	60	theme	energy	208:213	arg1	supply					198:203	an adequate supply	186:203	an adequate supply of energy	186:213	The synthesis of milk components in bovine mammary epithelial cells (BMEC) requires an adequate supply of energy.
31733862	10	61	theme	AMPK	1435:1438	arg1	Activation					1421:1430	Activation	1421:1430	Activation of AMPK	1421:1438	Activation of AMPK decreased lipid synthesis by 19%.
31733862	14	62	theme	substrate	1766:1774	arg1	availability					1776:1787	energy substrate availability	1759:1787	energy substrate availability	1759:1787	To assess the regulation of AMPK by energy substrate availability, we incubated BMEC in a control medium containing 4 mM D-glucose and 1 mM sodium acetate, or medium lacking glucose or acetate, for 4 h. Compared with the control medium, deprivation of glucose or acetate promoted AMPKα phosphorylation at Thr172 by 84% or 58%, respectively.
31733862	9	63	theme	AMPK	1301:1304	arg1	activation					1287:1296	activation	1287:1296	activation of AMPK	1287:1304	To assess the effect of AMPK activation on milk component synthesis, we treated BMEC with 100 μM A-769662 (A76; an allosteric activator of AMPK) or vehicle control for 16 h. Consistent with activation of AMPK, A76 increased phosphorylation of its downstream targets ACC Ser79 and TSC2 Ser1387 by 144% and 26%, respectively.
31733862	11	64	theme	FABP3	1529:1533	arg1	expression					1515:1524	increased expression	1505:1524	increased expression of FABP3	1505:1533	This effect was accompanied by increased expression of FABP3.
31733862	9	65	theme	AMPK	1121:1124	arg1	activation					1126:1135	AMPK activation	1121:1135	AMPK activation	1121:1135	To assess the effect of AMPK activation on milk component synthesis, we treated BMEC with 100 μM A-769662 (A76; an allosteric activator of AMPK) or vehicle control for 16 h. Consistent with activation of AMPK, A76 increased phosphorylation of its downstream targets ACC Ser79 and TSC2 Ser1387 by 144% and 26%, respectively.
31733862	2	66	theme	cellular	261:268	arg1	gauge					277:281	a cellular energy gauge	259:281	a cellular energy gauge that controls anabolic and catabolic processes to maintain a balance between energy supply and demand	259:383	The AMP-activated protein kinase (AMPK) is a cellular energy gauge that controls anabolic and catabolic processes to maintain a balance between energy supply and demand.
31733862	2	66	theme	cellular	261:268	arg1	kinase					242:247	The AMP-activated protein kinase	216:247	The AMP-activated protein kinase (AMPK)	216:254	The AMP-activated protein kinase (AMPK) is a cellular energy gauge that controls anabolic and catabolic processes to maintain a balance between energy supply and demand.
31733862	10	67	theme	lipid	1450:1454	arg1	synthesis					1456:1464	lipid synthesis	1450:1464	lipid synthesis	1450:1464	Activation of AMPK decreased lipid synthesis by 19%.
31733862	14	68	theme	energy	1759:1764	arg1	availability					1776:1787	energy substrate availability	1759:1787	energy substrate availability	1759:1787	To assess the regulation of AMPK by energy substrate availability, we incubated BMEC in a control medium containing 4 mM D-glucose and 1 mM sodium acetate, or medium lacking glucose or acetate, for 4 h. Compared with the control medium, deprivation of glucose or acetate promoted AMPKα phosphorylation at Thr172 by 84% or 58%, respectively.
31733862	3	69	dep	glucose	516:522	arg1	availability					536:547	availability	536:547	availability	536:547	The objectives of this study were to assess the role of AMPK on de novo lipid and lactose synthesis, as well as its regulation by glucose and acetate availability in BMEC.
31733862	0	70	theme	lactose	48:54	arg1	synthesis					56:64	lipid and lactose synthesis	38:64	lipid and lactose synthesis in bovine mammary epithelial cells	38:99	AMP-activated protein kinase controls lipid and lactose synthesis in bovine mammary epithelial cells.
31733862	6	71	theme	genes	834:838	arg1	expression					820:829	the expression	816:829	the expression of genes involved in lipid and lactose synthesis	816:878	We quantified the expression of genes involved in lipid and lactose synthesis using real-time quantitative PCR.
31733862	14	72	theme	acetate	1986:1992	arg1	deprivation					1960:1970	deprivation	1960:1970	deprivation of glucose or acetate	1960:1992	To assess the regulation of AMPK by energy substrate availability, we incubated BMEC in a control medium containing 4 mM D-glucose and 1 mM sodium acetate, or medium lacking glucose or acetate, for 4 h. Compared with the control medium, deprivation of glucose or acetate promoted AMPKα phosphorylation at Thr172 by 84% or 58%, respectively.
31733862	9	73	from	effect	1111:1116	arg1	synthesis					1155:1163	milk component synthesis	1140:1163	milk component synthesis	1140:1163	To assess the effect of AMPK activation on milk component synthesis, we treated BMEC with 100 μM A-769662 (A76; an allosteric activator of AMPK) or vehicle control for 16 h. Consistent with activation of AMPK, A76 increased phosphorylation of its downstream targets ACC Ser79 and TSC2 Ser1387 by 144% and 26%, respectively.
31733862	9	74	theme	component	1145:1153	arg1	synthesis					1155:1163	milk component synthesis	1140:1163	milk component synthesis	1140:1163	To assess the effect of AMPK activation on milk component synthesis, we treated BMEC with 100 μM A-769662 (A76; an allosteric activator of AMPK) or vehicle control for 16 h. Consistent with activation of AMPK, A76 increased phosphorylation of its downstream targets ACC Ser79 and TSC2 Ser1387 by 144% and 26%, respectively.
31733862	9	75	theme	targets	1355:1361	arg1	phosphorylation					1321:1335	phosphorylation	1321:1335	phosphorylation of its downstream targets ACC Ser79 and TSC2 Ser1387	1321:1388	To assess the effect of AMPK activation on milk component synthesis, we treated BMEC with 100 μM A-769662 (A76; an allosteric activator of AMPK) or vehicle control for 16 h. Consistent with activation of AMPK, A76 increased phosphorylation of its downstream targets ACC Ser79 and TSC2 Ser1387 by 144% and 26%, respectively.
31733862	14	76	theme	AMPK	1751:1754	arg1	regulation					1737:1746	the regulation	1733:1746	the regulation of AMPK by energy substrate availability	1733:1787	To assess the regulation of AMPK by energy substrate availability, we incubated BMEC in a control medium containing 4 mM D-glucose and 1 mM sodium acetate, or medium lacking glucose or acetate, for 4 h. Compared with the control medium, deprivation of glucose or acetate promoted AMPKα phosphorylation at Thr172 by 84% or 58%, respectively.
31733862	9	77	with	BMEC	1177:1180	arg1	control					1253:1259	vehicle control	1245:1259	vehicle control	1245:1259	To assess the effect of AMPK activation on milk component synthesis, we treated BMEC with 100 μM A-769662 (A76; an allosteric activator of AMPK) or vehicle control for 16 h. Consistent with activation of AMPK, A76 increased phosphorylation of its downstream targets ACC Ser79 and TSC2 Ser1387 by 144% and 26%, respectively.
31733862	9	77	with	BMEC	1177:1180	arg1	A-769662					1194:1201	100 μM A-769662	1187:1201	100 μM A-769662 (A76; an allosteric activator of AMPK)	1187:1240	To assess the effect of AMPK activation on milk component synthesis, we treated BMEC with 100 μM A-769662 (A76; an allosteric activator of AMPK) or vehicle control for 16 h. Consistent with activation of AMPK, A76 increased phosphorylation of its downstream targets ACC Ser79 and TSC2 Ser1387 by 144% and 26%, respectively.
31733862	2	78	theme	AMP-activated	220:232	arg1	kinase					242:247	The AMP-activated protein kinase	216:247	The AMP-activated protein kinase (AMPK)	216:254	The AMP-activated protein kinase (AMPK) is a cellular energy gauge that controls anabolic and catabolic processes to maintain a balance between energy supply and demand.
31733862	2	78	theme	AMP-activated	220:232	arg1	gauge					277:281	a cellular energy gauge	259:281	a cellular energy gauge that controls anabolic and catabolic processes to maintain a balance between energy supply and demand	259:383	The AMP-activated protein kinase (AMPK) is a cellular energy gauge that controls anabolic and catabolic processes to maintain a balance between energy supply and demand.
31733862	2	78	theme	AMP-activated	220:232	arg1	AMPK					250:253	AMPK	250:253	AMPK	250:253	The AMP-activated protein kinase (AMPK) is a cellular energy gauge that controls anabolic and catabolic processes to maintain a balance between energy supply and demand.
31733862	9	79	theme	TSC2	1377:1380	arg1	targets					1355:1361	its downstream targets	1340:1361	its downstream targets ACC Ser79 and TSC2 Ser1387	1340:1388	To assess the effect of AMPK activation on milk component synthesis, we treated BMEC with 100 μM A-769662 (A76; an allosteric activator of AMPK) or vehicle control for 16 h. Consistent with activation of AMPK, A76 increased phosphorylation of its downstream targets ACC Ser79 and TSC2 Ser1387 by 144% and 26%, respectively.
31733862	9	79	theme	TSC2	1377:1380	arg1	Ser1387					1382:1388	TSC2 Ser1387	1377:1388	TSC2 Ser1387	1377:1388	To assess the effect of AMPK activation on milk component synthesis, we treated BMEC with 100 μM A-769662 (A76; an allosteric activator of AMPK) or vehicle control for 16 h. Consistent with activation of AMPK, A76 increased phosphorylation of its downstream targets ACC Ser79 and TSC2 Ser1387 by 144% and 26%, respectively.
31733862	17	80	theme	milk	2359:2362	arg1	composition					2364:2374	milk composition	2359:2374	milk composition	2359:2374	Further studies are needed to assess the physiological relevance of AMPK activation for milk composition in vivo and to identify the mechanisms mediating its effects on milk component synthesis.
31733862	13	81	theme	AMPK	1611:1614	arg1	activation					1616:1625	AMPK activation	1611:1625	AMPK activation	1611:1625	In addition, AMPK activation reduced lactose synthesis by 24% and lowered the expression of SLC2A1, the gene encoding GLUT1.
31733862	6	82	theme	real-time	886:894	arg1	PCR					909:911	real-time quantitative PCR	886:911	real-time quantitative PCR	886:911	We quantified the expression of genes involved in lipid and lactose synthesis using real-time quantitative PCR.
31733862	17	83	theme	milk	2440:2443	arg1	synthesis					2455:2463	milk component synthesis	2440:2463	milk component synthesis	2440:2463	Further studies are needed to assess the physiological relevance of AMPK activation for milk composition in vivo and to identify the mechanisms mediating its effects on milk component synthesis.
31733862	7	84	theme	radioactive	982:992	arg1	substrates					994:1003	radioactive substrates	982:1003	radioactive substrates	982:1003	We measured de novo lipid and lactose synthesis by incorporation of radioactive substrates.
31733862	9	85	theme	μM	1191:1192	arg1	A-769662					1194:1201	100 μM A-769662	1187:1201	100 μM A-769662 (A76; an allosteric activator of AMPK)	1187:1240	To assess the effect of AMPK activation on milk component synthesis, we treated BMEC with 100 μM A-769662 (A76; an allosteric activator of AMPK) or vehicle control for 16 h. Consistent with activation of AMPK, A76 increased phosphorylation of its downstream targets ACC Ser79 and TSC2 Ser1387 by 144% and 26%, respectively.
31733862	1	86	theme	components	124:133	arg1	synthesis					106:114	The synthesis	102:114	The synthesis of milk components in bovine mammary epithelial cells (BMEC)	102:175	The synthesis of milk components in bovine mammary epithelial cells (BMEC) requires an adequate supply of energy.
31733862	12	87	theme	mature	1581:1586	arg1	SREBP-1c					1588:1595	mature SREBP-1c	1581:1595	mature SREBP-1c	1581:1595	Activation of AMPK reduced the proportion of mature SREBP-1c.
31733862	3	88	from	regulation	502:511	arg1	lipid					458:462	de novo lipid and lactose synthesis	450:484	lipid	458:462	The objectives of this study were to assess the role of AMPK on de novo lipid and lactose synthesis, as well as its regulation by glucose and acetate availability in BMEC.
31733862	3	88	from	regulation	502:511	arg1	synthesis					476:484	de novo lipid and lactose synthesis	450:484	synthesis	476:484	The objectives of this study were to assess the role of AMPK on de novo lipid and lactose synthesis, as well as its regulation by glucose and acetate availability in BMEC.
31733862	0	89	theme	bovine	69:74	arg1	cells					95:99	bovine mammary epithelial cells	69:99	bovine mammary epithelial cells	69:99	AMP-activated protein kinase controls lipid and lactose synthesis in bovine mammary epithelial cells.
31733862	7	90	theme	lactose	944:950	arg1	synthesis					952:960	de novo lipid and lactose synthesis	926:960	synthesis	952:960	We measured de novo lipid and lactose synthesis by incorporation of radioactive substrates.
31733862	1	91	theme	bovine	138:143	arg1	BMEC					171:174	BMEC	171:174	BMEC	171:174	The synthesis of milk components in bovine mammary epithelial cells (BMEC) requires an adequate supply of energy.
31733862	1	91	theme	bovine	138:143	arg1	cells					164:168	bovine mammary epithelial cells	138:168	bovine mammary epithelial cells (BMEC)	138:175	The synthesis of milk components in bovine mammary epithelial cells (BMEC) requires an adequate supply of energy.
31733862	0	92	theme	epithelial	84:93	arg1	cells					95:99	bovine mammary epithelial cells	69:99	bovine mammary epithelial cells	69:99	AMP-activated protein kinase controls lipid and lactose synthesis in bovine mammary epithelial cells.
31733862	1	93	theme	epithelial	153:162	arg1	BMEC					171:174	BMEC	171:174	BMEC	171:174	The synthesis of milk components in bovine mammary epithelial cells (BMEC) requires an adequate supply of energy.
31733862	1	93	theme	epithelial	153:162	arg1	cells					164:168	bovine mammary epithelial cells	138:168	bovine mammary epithelial cells (BMEC)	138:175	The synthesis of milk components in bovine mammary epithelial cells (BMEC) requires an adequate supply of energy.
31733862	4	94	theme	Holstein	622:629	arg1	cows					631:634	3 lactating Holstein cows	610:634	3 lactating Holstein cows	610:634	We isolated primary BMEC from the mammary tissue of 3 lactating Holstein cows and differentiated them with lactogenic hormones for 4 d.
31733862	9	95	theme	allosteric	1212:1221	arg1	activator					1223:1231	an allosteric activator	1209:1231	A76; an allosteric activator of AMPK	1204:1239	To assess the effect of AMPK activation on milk component synthesis, we treated BMEC with 100 μM A-769662 (A76; an allosteric activator of AMPK) or vehicle control for 16 h. Consistent with activation of AMPK, A76 increased phosphorylation of its downstream targets ACC Ser79 and TSC2 Ser1387 by 144% and 26%, respectively.
31733862	7	96	theme	de	926:927	arg1	lipid					934:938	de novo lipid and lactose synthesis	926:960	lipid	934:938	We measured de novo lipid and lactose synthesis by incorporation of radioactive substrates.
31733862	14	97	theme	sodium	1863:1868	arg1	acetate					1870:1876	1 mM sodium acetate	1858:1876	1 mM sodium acetate	1858:1876	To assess the regulation of AMPK by energy substrate availability, we incubated BMEC in a control medium containing 4 mM D-glucose and 1 mM sodium acetate, or medium lacking glucose or acetate, for 4 h. Compared with the control medium, deprivation of glucose or acetate promoted AMPKα phosphorylation at Thr172 by 84% or 58%, respectively.
31733862	0	98	from	synthesis	56:64	arg1	cells					95:99	bovine mammary epithelial cells	69:99	bovine mammary epithelial cells	69:99	AMP-activated protein kinase controls lipid and lactose synthesis in bovine mammary epithelial cells.
31733862	17	99	theme	physiological	2312:2324	arg1	relevance					2326:2334	the physiological relevance	2308:2334	the physiological relevance of AMPK activation for milk composition	2308:2374	Further studies are needed to assess the physiological relevance of AMPK activation for milk composition in vivo and to identify the mechanisms mediating its effects on milk component synthesis.
31733862	2	100	theme	anabolic	297:304	arg1	processes					320:328	anabolic and catabolic processes	297:328	anabolic and catabolic processes	297:328	The AMP-activated protein kinase (AMPK) is a cellular energy gauge that controls anabolic and catabolic processes to maintain a balance between energy supply and demand.
31733862	16	101	dep	de	2226:2227	arg1	novo					2229:2232	novo	2229:2232	novo	2229:2232	We concluded that activation of AMPK inhibits de novo lipid and lactose synthesis in BMEC.
31733862	14	102	theme	1	1858:1858	arg1	mM					1860:1861	mM	1860:1861	mM	1860:1861	To assess the regulation of AMPK by energy substrate availability, we incubated BMEC in a control medium containing 4 mM D-glucose and 1 mM sodium acetate, or medium lacking glucose or acetate, for 4 h. Compared with the control medium, deprivation of glucose or acetate promoted AMPKα phosphorylation at Thr172 by 84% or 58%, respectively.
31733862	0	103	theme	protein	14:20	arg1	kinase					22:27	AMP-activated protein kinase	0:27	AMP-activated protein kinase	0:27	AMP-activated protein kinase controls lipid and lactose synthesis in bovine mammary epithelial cells.
31733862	17	104	theme	activation	2344:2353	arg1	relevance					2326:2334	the physiological relevance	2308:2334	the physiological relevance of AMPK activation for milk composition	2308:2374	Further studies are needed to assess the physiological relevance of AMPK activation for milk composition in vivo and to identify the mechanisms mediating its effects on milk component synthesis.
31733862	4	105	theme	lactogenic	665:674	arg1	hormones					676:683	lactogenic hormones	665:683	lactogenic hormones for 4 d	665:691	We isolated primary BMEC from the mammary tissue of 3 lactating Holstein cows and differentiated them with lactogenic hormones for 4 d.
31733862	3	106	from	glucose	516:522	arg1	BMEC					552:555	BMEC	552:555	BMEC	552:555	The objectives of this study were to assess the role of AMPK on de novo lipid and lactose synthesis, as well as its regulation by glucose and acetate availability in BMEC.
31733862	9	107	theme	vehicle	1245:1251	arg1	control					1253:1259	vehicle control	1245:1259	vehicle control	1245:1259	To assess the effect of AMPK activation on milk component synthesis, we treated BMEC with 100 μM A-769662 (A76; an allosteric activator of AMPK) or vehicle control for 16 h. Consistent with activation of AMPK, A76 increased phosphorylation of its downstream targets ACC Ser79 and TSC2 Ser1387 by 144% and 26%, respectively.
31733862	14	108	theme	4	1839:1839	arg1	mM					1841:1842	mM	1841:1842	mM	1841:1842	To assess the regulation of AMPK by energy substrate availability, we incubated BMEC in a control medium containing 4 mM D-glucose and 1 mM sodium acetate, or medium lacking glucose or acetate, for 4 h. Compared with the control medium, deprivation of glucose or acetate promoted AMPKα phosphorylation at Thr172 by 84% or 58%, respectively.
31733862	9	109	dep	A76	1204:1206	arg1	activator					1223:1231	an allosteric activator	1209:1231	A76; an allosteric activator of AMPK	1204:1239	To assess the effect of AMPK activation on milk component synthesis, we treated BMEC with 100 μM A-769662 (A76; an allosteric activator of AMPK) or vehicle control for 16 h. Consistent with activation of AMPK, A76 increased phosphorylation of its downstream targets ACC Ser79 and TSC2 Ser1387 by 144% and 26%, respectively.
31733862	3	110	from	acetate	528:534	arg1	BMEC					552:555	BMEC	552:555	BMEC	552:555	The objectives of this study were to assess the role of AMPK on de novo lipid and lactose synthesis, as well as its regulation by glucose and acetate availability in BMEC.
31733862	5	111	theme	protein	706:712	arg1	abundance					714:722	protein abundance	706:722	protein abundance	706:722	We measured protein abundance, site-specific phosphorylation, and proteolytic processing by immunoblotting.
31733862	8	112	theme	complete	1051:1058	arg1	block					1060:1064	complete block	1051:1064	a randomized complete block design	1038:1071	We analyzed data by ANOVA using a randomized complete block design with PROC MIXED in SAS.
31733862	16	113	theme	AMPK	2212:2215	arg1	activation					2198:2207	activation	2198:2207	activation of AMPK	2198:2215	We concluded that activation of AMPK inhibits de novo lipid and lactose synthesis in BMEC.
31733862	2	114	theme	catabolic	310:318	arg1	processes					320:328	anabolic and catabolic processes	297:328	anabolic and catabolic processes	297:328	The AMP-activated protein kinase (AMPK) is a cellular energy gauge that controls anabolic and catabolic processes to maintain a balance between energy supply and demand.
31733862	3	115	theme	AMPK	442:445	arg1	regulation					502:511	its regulation	498:511	its regulation by glucose and acetate availability in BMEC	498:555	The objectives of this study were to assess the role of AMPK on de novo lipid and lactose synthesis, as well as its regulation by glucose and acetate availability in BMEC.
31733862	3	115	theme	AMPK	442:445	arg1	role					434:437	the role	430:437	the role of AMPK on de novo lipid and lactose synthesis	430:484	The objectives of this study were to assess the role of AMPK on de novo lipid and lactose synthesis, as well as its regulation by glucose and acetate availability in BMEC.
31733862	17	116	theme	Further	2271:2277	arg1	studies					2279:2285	Further studies	2271:2285	Further studies	2271:2285	Further studies are needed to assess the physiological relevance of AMPK activation for milk composition in vivo and to identify the mechanisms mediating its effects on milk component synthesis.
31733862	3	117	theme	lactose	468:474	arg1	synthesis					476:484	de novo lipid and lactose synthesis	450:484	synthesis	476:484	The objectives of this study were to assess the role of AMPK on de novo lipid and lactose synthesis, as well as its regulation by glucose and acetate availability in BMEC.
30572166	7	0	theme	components	1476:1485	arg1	DNA					1411:1413	DNA	1411:1413	DNA (0.4 ± 0.4 µg/mg dry-weight)	1411:1442	VH was successfully extracted, exhibiting negligible amounts of DNA (0.4 ± 0.4 µg/mg dry-weight) and notable preservation of ECM components.
30572166	7	0	theme	components	1476:1485	arg1	dry-weight					1432:1441	0.4 ± 0.4 µg/mg dry-weight	1416:1441	0.4 ± 0.4 µg/mg dry-weight	1416:1441	VH was successfully extracted, exhibiting negligible amounts of DNA (0.4 ± 0.4 µg/mg dry-weight) and notable preservation of ECM components.
30572166	7	0	theme	components	1476:1485	arg1	amounts					1400:1406	negligible amounts	1389:1406	negligible amounts of DNA (0.4 ± 0.4 µg/mg dry-weight)	1389:1442	VH was successfully extracted, exhibiting negligible amounts of DNA (0.4 ± 0.4 µg/mg dry-weight) and notable preservation of ECM components.
30572166	7	0	theme	components	1476:1485	arg1	preservation					1456:1467	notable preservation	1448:1467	notable preservation of ECM components	1448:1485	VH was successfully extracted, exhibiting negligible amounts of DNA (0.4 ± 0.4 µg/mg dry-weight) and notable preservation of ECM components.
30572166	7	0	theme	components	1476:1485	arg1	components					1476:1485	ECM components	1472:1485	ECM components	1472:1485	VH was successfully extracted, exhibiting negligible amounts of DNA (0.4 ± 0.4 µg/mg dry-weight) and notable preservation of ECM components.
30572166	9	1	theme	TE	1923:1924	arg1	applications					1926:1937	potential TE applications	1913:1937	potential TE applications	1913:1937	Interestingly, encapsulated cells self-assembled the VH-hydrogel into spheroids, resulting in uniform distribution of both GAGs and collagen type II with increased compressive mechanical properties, rendering VH a permissive native ECM source to fabricate cartilaginous hydrogels for potential TE applications.
30572166	3	2	theme	cartilage	636:644	arg1	GAG					603:605	GAG	603:605	GAG	603:605	In this study, we demonstrate for the first time the successful application of vitreous humor (VH), a highly hydrated tissue closely resembling the glycosaminoglycan (GAG) and collagen composition of cartilage, as an ECM hydrogel to support chondrogenic differentiation.
30572166	3	2	theme	cartilage	636:644	arg1	glycosaminoglycan					584:600	the glycosaminoglycan	580:600	the glycosaminoglycan (GAG)	580:606	In this study, we demonstrate for the first time the successful application of vitreous humor (VH), a highly hydrated tissue closely resembling the glycosaminoglycan (GAG) and collagen composition of cartilage, as an ECM hydrogel to support chondrogenic differentiation.
30572166	3	2	theme	cartilage	636:644	arg1	composition					621:631	collagen composition	612:631	collagen composition of cartilage	612:644	In this study, we demonstrate for the first time the successful application of vitreous humor (VH), a highly hydrated tissue closely resembling the glycosaminoglycan (GAG) and collagen composition of cartilage, as an ECM hydrogel to support chondrogenic differentiation.
30572166	3	3	theme	first	474:478	arg1	time					480:483	the first time	470:483	the first time	470:483	In this study, we demonstrate for the first time the successful application of vitreous humor (VH), a highly hydrated tissue closely resembling the glycosaminoglycan (GAG) and collagen composition of cartilage, as an ECM hydrogel to support chondrogenic differentiation.
30572166	6	4	theme	chondrogenic	1152:1163	arg1	aggrecan					1182:1189	aggrecan	1182:1189	aggrecan	1182:1189	Viability, metabolic activity, GAG and DNA content, chondrogenic gene expression (aggrecan, collagen I/II mRNA) and mechanical properties were quantified and matrix deposition was visualised using immunohistochemistry (Safranin-O, collagen I/II).
30572166	6	4	theme	chondrogenic	1152:1163	arg1	expression					1170:1179	chondrogenic gene expression	1152:1179	chondrogenic gene expression (aggrecan, collagen I/II mRNA)	1152:1210	Viability, metabolic activity, GAG and DNA content, chondrogenic gene expression (aggrecan, collagen I/II mRNA) and mechanical properties were quantified and matrix deposition was visualised using immunohistochemistry (Safranin-O, collagen I/II).
30572166	11	5	theme	vitreous	2393:2400	arg1	VH					2409:2410	VH	2409:2410	VH	2409:2410	In this study, we present an alternative route to fabricate highly permissive and bioactive ECM hydrogels from vitreous humor (VH) tissue.
30572166	11	5	theme	vitreous	2393:2400	arg1	humor					2402:2406	vitreous humor	2393:2406	vitreous humor (VH) tissue	2393:2418	In this study, we present an alternative route to fabricate highly permissive and bioactive ECM hydrogels from vitreous humor (VH) tissue.
30572166	10	6	theme	cell-instructive	1993:2008	arg1	matrix					2034:2039	Fabricating bioactive and cell-instructive cartilage extracellular matrix	1967:2039	SIGNIFICANCE: Fabricating bioactive and cell-instructive cartilage extracellular matrix (ECM)	1953:2045	STATEMENT OF SIGNIFICANCE: Fabricating bioactive and cell-instructive cartilage extracellular matrix (ECM) derived biomaterials and hydrogels has over recent years proven to be a challenging task, often limited by poor retention of inherent environmental cues post decellularisation due to the dense and avascular nature of native cartilage.
30572166	10	6	theme	cell-instructive	1993:2008	arg1	ECM					2042:2044	ECM	2042:2044	ECM	2042:2044	STATEMENT OF SIGNIFICANCE: Fabricating bioactive and cell-instructive cartilage extracellular matrix (ECM) derived biomaterials and hydrogels has over recent years proven to be a challenging task, often limited by poor retention of inherent environmental cues post decellularisation due to the dense and avascular nature of native cartilage.
30572166	9	7	dep	VH	1838:1839	arg1	source					1865:1870	a permissive native ECM source	1841:1870	VH a permissive native ECM source	1838:1870	Interestingly, encapsulated cells self-assembled the VH-hydrogel into spheroids, resulting in uniform distribution of both GAGs and collagen type II with increased compressive mechanical properties, rendering VH a permissive native ECM source to fabricate cartilaginous hydrogels for potential TE applications.
30572166	3	8	theme	vitreous	515:522	arg1	VH					531:532	VH	531:532	VH	531:532	In this study, we demonstrate for the first time the successful application of vitreous humor (VH), a highly hydrated tissue closely resembling the glycosaminoglycan (GAG) and collagen composition of cartilage, as an ECM hydrogel to support chondrogenic differentiation.
30572166	3	8	theme	vitreous	515:522	arg1	tissue					554:559	a highly hydrated tissue	536:559	a highly hydrated tissue closely resembling the glycosaminoglycan (GAG) and collagen composition of cartilage	536:644	In this study, we demonstrate for the first time the successful application of vitreous humor (VH), a highly hydrated tissue closely resembling the glycosaminoglycan (GAG) and collagen composition of cartilage, as an ECM hydrogel to support chondrogenic differentiation.
30572166	3	8	theme	vitreous	515:522	arg1	humor					524:528	vitreous humor	515:528	vitreous humor (VH)	515:533	In this study, we demonstrate for the first time the successful application of vitreous humor (VH), a highly hydrated tissue closely resembling the glycosaminoglycan (GAG) and collagen composition of cartilage, as an ECM hydrogel to support chondrogenic differentiation.
30572166	13	9	theme	hybrid	2841:2846	arg1	biomaterials					2848:2859	responsive hybrid biomaterials	2830:2859	responsive hybrid biomaterials	2830:2859	The work showcases a naturally intact and unmodified hydrogel design that improves cellular responses and may help guide the development of cell instructive and stimuli responsive hybrid biomaterials in a number of TERM applications.
30572166	7	10	theme	notable	1448:1454	arg1	preservation					1456:1467	notable preservation	1448:1467	notable preservation of ECM components	1448:1485	VH was successfully extracted, exhibiting negligible amounts of DNA (0.4 ± 0.4 µg/mg dry-weight) and notable preservation of ECM components.
30572166	4	11	theme	human	813:817	arg1	chondrocytes					872:883	human articular chondrocytes	856:883	human articular chondrocytes	856:883	Equine VH was extracted followed by biochemical quantifications, histological examinations, cytotoxicity (human mesenchymal stromal cells, hMSCs and human articular chondrocytes, hACs) and U937 cell proliferation studies.
30572166	4	11	theme	human	813:817	arg1	hMSCs					846:850	hMSCs	846:850	hMSCs	846:850	Equine VH was extracted followed by biochemical quantifications, histological examinations, cytotoxicity (human mesenchymal stromal cells, hMSCs and human articular chondrocytes, hACs) and U937 cell proliferation studies.
30572166	4	11	theme	human	813:817	arg1	cells					839:843	human mesenchymal stromal cells	813:843	human mesenchymal stromal cells	813:843	Equine VH was extracted followed by biochemical quantifications, histological examinations, cytotoxicity (human mesenchymal stromal cells, hMSCs and human articular chondrocytes, hACs) and U937 cell proliferation studies.
30572166	4	11	theme	human	813:817	arg1	cytotoxicity					799:810	cytotoxicity	799:810	cytotoxicity (human mesenchymal stromal cells, hMSCs and human articular chondrocytes, hACs)	799:890	Equine VH was extracted followed by biochemical quantifications, histological examinations, cytotoxicity (human mesenchymal stromal cells, hMSCs and human articular chondrocytes, hACs) and U937 cell proliferation studies.
30572166	12	12	theme	components	2573:2582	arg1	fabrication					2537:2547	fabrication	2537:2547	fabrication of cartilaginous matrix components	2537:2582	This paper specifically reports the discovery of optimal VH extraction protocols and cell seeding strategy enabling fabrication of cartilaginous matrix components into a hydrogel support material for promoting chondrogenic differentiation.
30572166	3	13	theme	ECM	653:655	arg1	hydrogel					657:664	an ECM hydrogel	650:664	an ECM hydrogel to support chondrogenic differentiation	650:704	In this study, we demonstrate for the first time the successful application of vitreous humor (VH), a highly hydrated tissue closely resembling the glycosaminoglycan (GAG) and collagen composition of cartilage, as an ECM hydrogel to support chondrogenic differentiation.
30572166	3	13	theme	ECM	653:655	arg1	application					500:510	the successful application	485:510	the successful application of vitreous humor (VH), a highly hydrated tissue closely resembling the glycosaminoglycan (GAG) and collagen composition of cartilage,	485:645	In this study, we demonstrate for the first time the successful application of vitreous humor (VH), a highly hydrated tissue closely resembling the glycosaminoglycan (GAG) and collagen composition of cartilage, as an ECM hydrogel to support chondrogenic differentiation.
30572166	6	14	theme	mechanical	1216:1225	arg1	properties					1227:1236	mechanical properties	1216:1236	mechanical properties	1216:1236	Viability, metabolic activity, GAG and DNA content, chondrogenic gene expression (aggrecan, collagen I/II mRNA) and mechanical properties were quantified and matrix deposition was visualised using immunohistochemistry (Safranin-O, collagen I/II).
30572166	1	15	theme	exciting	184:191	arg1	opportunities					217:229	exciting tissue engineering (TE) opportunities	184:229	exciting tissue engineering (TE) opportunities	184:229	Decellularisation of tissues, utilising their biochemical cues, poses exciting tissue engineering (TE) opportunities.
30572166	4	16	theme	stromal	831:837	arg1	chondrocytes					872:883	human articular chondrocytes	856:883	human articular chondrocytes	856:883	Equine VH was extracted followed by biochemical quantifications, histological examinations, cytotoxicity (human mesenchymal stromal cells, hMSCs and human articular chondrocytes, hACs) and U937 cell proliferation studies.
30572166	4	16	theme	stromal	831:837	arg1	hMSCs					846:850	hMSCs	846:850	hMSCs	846:850	Equine VH was extracted followed by biochemical quantifications, histological examinations, cytotoxicity (human mesenchymal stromal cells, hMSCs and human articular chondrocytes, hACs) and U937 cell proliferation studies.
30572166	4	16	theme	stromal	831:837	arg1	cells					839:843	human mesenchymal stromal cells	813:843	human mesenchymal stromal cells	813:843	Equine VH was extracted followed by biochemical quantifications, histological examinations, cytotoxicity (human mesenchymal stromal cells, hMSCs and human articular chondrocytes, hACs) and U937 cell proliferation studies.
30572166	4	16	theme	stromal	831:837	arg1	cytotoxicity					799:810	cytotoxicity	799:810	cytotoxicity (human mesenchymal stromal cells, hMSCs and human articular chondrocytes, hACs)	799:890	Equine VH was extracted followed by biochemical quantifications, histological examinations, cytotoxicity (human mesenchymal stromal cells, hMSCs and human articular chondrocytes, hACs) and U937 cell proliferation studies.
30572166	12	17	theme	extraction	2481:2490	arg1	protocols					2492:2500	optimal VH extraction protocols	2470:2500	optimal VH extraction protocols	2470:2500	This paper specifically reports the discovery of optimal VH extraction protocols and cell seeding strategy enabling fabrication of cartilaginous matrix components into a hydrogel support material for promoting chondrogenic differentiation.
30572166	7	18	theme	negligible	1389:1398	arg1	DNA					1411:1413	DNA	1411:1413	DNA (0.4 ± 0.4 µg/mg dry-weight)	1411:1442	VH was successfully extracted, exhibiting negligible amounts of DNA (0.4 ± 0.4 µg/mg dry-weight) and notable preservation of ECM components.
30572166	7	18	theme	negligible	1389:1398	arg1	dry-weight					1432:1441	0.4 ± 0.4 µg/mg dry-weight	1416:1441	0.4 ± 0.4 µg/mg dry-weight	1416:1441	VH was successfully extracted, exhibiting negligible amounts of DNA (0.4 ± 0.4 µg/mg dry-weight) and notable preservation of ECM components.
30572166	7	18	theme	negligible	1389:1398	arg1	amounts					1400:1406	negligible amounts	1389:1406	negligible amounts of DNA (0.4 ± 0.4 µg/mg dry-weight)	1389:1442	VH was successfully extracted, exhibiting negligible amounts of DNA (0.4 ± 0.4 µg/mg dry-weight) and notable preservation of ECM components.
30572166	7	18	theme	negligible	1389:1398	arg1	components					1476:1485	ECM components	1472:1485	ECM components	1472:1485	VH was successfully extracted, exhibiting negligible amounts of DNA (0.4 ± 0.4 µg/mg dry-weight) and notable preservation of ECM components.
30572166	9	19	theme	GAGs	1752:1755	arg1	distribution					1731:1742	uniform distribution	1723:1742	uniform distribution of both GAGs and collagen type II	1723:1776	Interestingly, encapsulated cells self-assembled the VH-hydrogel into spheroids, resulting in uniform distribution of both GAGs and collagen type II with increased compressive mechanical properties, rendering VH a permissive native ECM source to fabricate cartilaginous hydrogels for potential TE applications.
30572166	1	20	theme	engineering	200:210	arg1	opportunities					217:229	exciting tissue engineering (TE) opportunities	184:229	exciting tissue engineering (TE) opportunities	184:229	Decellularisation of tissues, utilising their biochemical cues, poses exciting tissue engineering (TE) opportunities.
30572166	11	21	theme	permissive	2349:2358	arg1	hydrogels					2378:2386	highly permissive and bioactive ECM hydrogels	2342:2386	highly permissive and bioactive ECM hydrogels	2342:2386	In this study, we present an alternative route to fabricate highly permissive and bioactive ECM hydrogels from vitreous humor (VH) tissue.
30572166	3	22	theme	chondrogenic	677:688	arg1	differentiation					690:704	chondrogenic differentiation	677:704	chondrogenic differentiation	677:704	In this study, we demonstrate for the first time the successful application of vitreous humor (VH), a highly hydrated tissue closely resembling the glycosaminoglycan (GAG) and collagen composition of cartilage, as an ECM hydrogel to support chondrogenic differentiation.
30572166	10	23	link	derived	2047:2053	arg1	biomaterials					2055:2066	SIGNIFICANCE: Fabricating bioactive and cell-instructive cartilage extracellular matrix (ECM) derived biomaterials	1953:2066	SIGNIFICANCE: Fabricating bioactive and cell-instructive cartilage extracellular matrix (ECM) derived biomaterials	1953:2066	STATEMENT OF SIGNIFICANCE: Fabricating bioactive and cell-instructive cartilage extracellular matrix (ECM) derived biomaterials and hydrogels has over recent years proven to be a challenging task, often limited by poor retention of inherent environmental cues post decellularisation due to the dense and avascular nature of native cartilage.
30572166	4	24	theme	U937	896:899	arg1	studies					920:926	U937 cell proliferation studies	896:926	U937 cell proliferation studies	896:926	Equine VH was extracted followed by biochemical quantifications, histological examinations, cytotoxicity (human mesenchymal stromal cells, hMSCs and human articular chondrocytes, hACs) and U937 cell proliferation studies.
30572166	10	25	theme	environmental	2181:2193	arg1	cues					2195:2198	inherent environmental cues	2172:2198	inherent environmental cues	2172:2198	STATEMENT OF SIGNIFICANCE: Fabricating bioactive and cell-instructive cartilage extracellular matrix (ECM) derived biomaterials and hydrogels has over recent years proven to be a challenging task, often limited by poor retention of inherent environmental cues post decellularisation due to the dense and avascular nature of native cartilage.
30572166	10	26	theme	biomaterials	2055:2066	arg1	STATEMENT					1940:1948	STATEMENT	1940:1948	STATEMENT OF SIGNIFICANCE: Fabricating bioactive and cell-instructive cartilage extracellular matrix (ECM) derived biomaterials and hydrogels	1940:2080	STATEMENT OF SIGNIFICANCE: Fabricating bioactive and cell-instructive cartilage extracellular matrix (ECM) derived biomaterials and hydrogels has over recent years proven to be a challenging task, often limited by poor retention of inherent environmental cues post decellularisation due to the dense and avascular nature of native cartilage.
30572166	10	26	theme	biomaterials	2055:2066	arg1	task					2131:2134	a challenging task	2117:2134	a challenging task	2117:2134	STATEMENT OF SIGNIFICANCE: Fabricating bioactive and cell-instructive cartilage extracellular matrix (ECM) derived biomaterials and hydrogels has over recent years proven to be a challenging task, often limited by poor retention of inherent environmental cues post decellularisation due to the dense and avascular nature of native cartilage.
30572166	5	27	theme	micro-tissue	1045:1056	arg1	cultures					1065:1072	scaffold-free micro-tissue pellet cultures	1031:1072	scaffold-free micro-tissue pellet cultures	1031:1072	VH was further seeded with hACs or hMSCs and cultured for 3-weeks to study chondrogenesis compared to scaffold-free micro-tissue pellet cultures and collagen-I hydrogels.
30572166	3	28	theme	hydrated	545:552	arg1	humor					524:528	vitreous humor	515:528	vitreous humor (VH)	515:533	In this study, we demonstrate for the first time the successful application of vitreous humor (VH), a highly hydrated tissue closely resembling the glycosaminoglycan (GAG) and collagen composition of cartilage, as an ECM hydrogel to support chondrogenic differentiation.
30572166	3	28	theme	hydrated	545:552	arg1	tissue					554:559	a highly hydrated tissue	536:559	a highly hydrated tissue closely resembling the glycosaminoglycan (GAG) and collagen composition of cartilage	536:644	In this study, we demonstrate for the first time the successful application of vitreous humor (VH), a highly hydrated tissue closely resembling the glycosaminoglycan (GAG) and collagen composition of cartilage, as an ECM hydrogel to support chondrogenic differentiation.
30572166	4	29	theme	articular	862:870	arg1	chondrocytes					872:883	human articular chondrocytes	856:883	human articular chondrocytes	856:883	Equine VH was extracted followed by biochemical quantifications, histological examinations, cytotoxicity (human mesenchymal stromal cells, hMSCs and human articular chondrocytes, hACs) and U937 cell proliferation studies.
30572166	4	29	theme	articular	862:870	arg1	cells					839:843	human mesenchymal stromal cells	813:843	human mesenchymal stromal cells	813:843	Equine VH was extracted followed by biochemical quantifications, histological examinations, cytotoxicity (human mesenchymal stromal cells, hMSCs and human articular chondrocytes, hACs) and U937 cell proliferation studies.
30572166	6	30	theme	collagen	1192:1199	arg1	mRNA					1206:1209	collagen I/II mRNA	1192:1209	collagen I/II mRNA	1192:1209	Viability, metabolic activity, GAG and DNA content, chondrogenic gene expression (aggrecan, collagen I/II mRNA) and mechanical properties were quantified and matrix deposition was visualised using immunohistochemistry (Safranin-O, collagen I/II).
30572166	1	31	theme	tissues	135:141	arg1	Decellularisation					114:130	Decellularisation	114:130	Decellularisation	114:130	Decellularisation of tissues, utilising their biochemical cues, poses exciting tissue engineering (TE) opportunities.
30572166	13	32	from	development	2786:2796	arg1	number					2866:2871	a number	2864:2871	a number of TERM applications	2864:2892	The work showcases a naturally intact and unmodified hydrogel design that improves cellular responses and may help guide the development of cell instructive and stimuli responsive hybrid biomaterials in a number of TERM applications.
30572166	10	33	theme	due	2223:2225	arg1	decellularisation					2205:2221	decellularisation	2205:2221	decellularisation due to the dense and avascular nature of native cartilage	2205:2279	STATEMENT OF SIGNIFICANCE: Fabricating bioactive and cell-instructive cartilage extracellular matrix (ECM) derived biomaterials and hydrogels has over recent years proven to be a challenging task, often limited by poor retention of inherent environmental cues post decellularisation due to the dense and avascular nature of native cartilage.
30572166	6	34	dep	aggrecan	1182:1189	arg1	mRNA					1206:1209	collagen I/II mRNA	1192:1209	collagen I/II mRNA	1192:1209	Viability, metabolic activity, GAG and DNA content, chondrogenic gene expression (aggrecan, collagen I/II mRNA) and mechanical properties were quantified and matrix deposition was visualised using immunohistochemistry (Safranin-O, collagen I/II).
30572166	9	35	theme	type	1770:1773	arg1	distribution					1731:1742	uniform distribution	1723:1742	uniform distribution of both GAGs and collagen type II	1723:1776	Interestingly, encapsulated cells self-assembled the VH-hydrogel into spheroids, resulting in uniform distribution of both GAGs and collagen type II with increased compressive mechanical properties, rendering VH a permissive native ECM source to fabricate cartilaginous hydrogels for potential TE applications.
30572166	10	36	theme	hydrogels	2072:2080	arg1	STATEMENT					1940:1948	STATEMENT	1940:1948	STATEMENT OF SIGNIFICANCE: Fabricating bioactive and cell-instructive cartilage extracellular matrix (ECM) derived biomaterials and hydrogels	1940:2080	STATEMENT OF SIGNIFICANCE: Fabricating bioactive and cell-instructive cartilage extracellular matrix (ECM) derived biomaterials and hydrogels has over recent years proven to be a challenging task, often limited by poor retention of inherent environmental cues post decellularisation due to the dense and avascular nature of native cartilage.
30572166	10	36	theme	hydrogels	2072:2080	arg1	task					2131:2134	a challenging task	2117:2134	a challenging task	2117:2134	STATEMENT OF SIGNIFICANCE: Fabricating bioactive and cell-instructive cartilage extracellular matrix (ECM) derived biomaterials and hydrogels has over recent years proven to be a challenging task, often limited by poor retention of inherent environmental cues post decellularisation due to the dense and avascular nature of native cartilage.
30572166	4	37	dep	extracted	721:729	arg1	followed					731:738	followed	731:738	followed by biochemical quantifications, histological examinations, cytotoxicity (human mesenchymal stromal cells, hMSCs and human articular chondrocytes, hACs) and U937 cell proliferation studies	731:926	Equine VH was extracted followed by biochemical quantifications, histological examinations, cytotoxicity (human mesenchymal stromal cells, hMSCs and human articular chondrocytes, hACs) and U937 cell proliferation studies.
30572166	0	38	theme	cartilage	71:79	arg1	applications					100:111	cartilage tissue engineering applications	71:111	cartilage tissue engineering applications	71:111	Intact vitreous humor as a potential extracellular matrix hydrogel for cartilage tissue engineering applications.
30572166	12	39	dep	discovery	2457:2465	arg1	strategy					2519:2526	strategy	2519:2526	strategy	2519:2526	This paper specifically reports the discovery of optimal VH extraction protocols and cell seeding strategy enabling fabrication of cartilaginous matrix components into a hydrogel support material for promoting chondrogenic differentiation.
30572166	13	40	theme	applications	2881:2892	arg1	number					2866:2871	a number	2864:2871	a number of TERM applications	2864:2892	The work showcases a naturally intact and unmodified hydrogel design that improves cellular responses and may help guide the development of cell instructive and stimuli responsive hybrid biomaterials in a number of TERM applications.
30572166	4	41	theme	proliferation	906:918	arg1	studies					920:926	U937 cell proliferation studies	896:926	U937 cell proliferation studies	896:926	Equine VH was extracted followed by biochemical quantifications, histological examinations, cytotoxicity (human mesenchymal stromal cells, hMSCs and human articular chondrocytes, hACs) and U937 cell proliferation studies.
30572166	10	42	dep	derived	2047:2053	arg1	SIGNIFICANCE					1953:1964	SIGNIFICANCE	1953:1964	SIGNIFICANCE: Fabricating bioactive and cell-instructive cartilage extracellular matrix (ECM)	1953:2045	STATEMENT OF SIGNIFICANCE: Fabricating bioactive and cell-instructive cartilage extracellular matrix (ECM) derived biomaterials and hydrogels has over recent years proven to be a challenging task, often limited by poor retention of inherent environmental cues post decellularisation due to the dense and avascular nature of native cartilage.
30572166	0	43	theme	engineering	88:98	arg1	applications					100:111	cartilage tissue engineering applications	71:111	cartilage tissue engineering applications	71:111	Intact vitreous humor as a potential extracellular matrix hydrogel for cartilage tissue engineering applications.
30572166	8	44	theme	cytotoxic	1509:1517	arg1	responses					1519:1527	cytotoxic responses	1509:1527	cytotoxic responses	1509:1527	VH displayed neither cytotoxic responses nor proliferation of macrophage-like U937 cells, instead enhancing both hMSC and hAC proliferation.
30572166	10	45	theme	native	2264:2269	arg1	cartilage					2271:2279	native cartilage	2264:2279	native cartilage	2264:2279	STATEMENT OF SIGNIFICANCE: Fabricating bioactive and cell-instructive cartilage extracellular matrix (ECM) derived biomaterials and hydrogels has over recent years proven to be a challenging task, often limited by poor retention of inherent environmental cues post decellularisation due to the dense and avascular nature of native cartilage.
30572166	6	46	theme	collagen	1331:1338	arg1	Safranin-O					1319:1328	Safranin-O	1319:1328	Safranin-O	1319:1328	Viability, metabolic activity, GAG and DNA content, chondrogenic gene expression (aggrecan, collagen I/II mRNA) and mechanical properties were quantified and matrix deposition was visualised using immunohistochemistry (Safranin-O, collagen I/II).
30572166	6	46	theme	collagen	1331:1338	arg1	I/II					1340:1343	collagen I/II	1331:1343	collagen I/II	1331:1343	Viability, metabolic activity, GAG and DNA content, chondrogenic gene expression (aggrecan, collagen I/II mRNA) and mechanical properties were quantified and matrix deposition was visualised using immunohistochemistry (Safranin-O, collagen I/II).
30572166	5	47	theme	collagen-I	1078:1087	arg1	hydrogels					1089:1097	collagen-I hydrogels	1078:1097	collagen-I hydrogels	1078:1097	VH was further seeded with hACs or hMSCs and cultured for 3-weeks to study chondrogenesis compared to scaffold-free micro-tissue pellet cultures and collagen-I hydrogels.
30572166	12	48	theme	protocols	2492:2500	arg1	discovery					2457:2465	the discovery	2453:2465	the discovery of optimal VH extraction protocols	2453:2500	This paper specifically reports the discovery of optimal VH extraction protocols and cell seeding strategy enabling fabrication of cartilaginous matrix components into a hydrogel support material for promoting chondrogenic differentiation.
30572166	12	48	theme	protocols	2492:2500	arg1	seeding					2511:2517	cell seeding	2506:2517	cell seeding	2506:2517	This paper specifically reports the discovery of optimal VH extraction protocols and cell seeding strategy enabling fabrication of cartilaginous matrix components into a hydrogel support material for promoting chondrogenic differentiation.
30572166	12	49	theme	chondrogenic	2631:2642	arg1	differentiation					2644:2658	chondrogenic differentiation	2631:2658	chondrogenic differentiation	2631:2658	This paper specifically reports the discovery of optimal VH extraction protocols and cell seeding strategy enabling fabrication of cartilaginous matrix components into a hydrogel support material for promoting chondrogenic differentiation.
30572166	0	50	theme	Intact	0:5	arg1	humor					16:20	Intact vitreous humor	0:20	Intact vitreous humor	0:20	Intact vitreous humor as a potential extracellular matrix hydrogel for cartilage tissue engineering applications.
30572166	12	51	theme	VH	2478:2479	arg1	protocols					2492:2500	optimal VH extraction protocols	2470:2500	optimal VH extraction protocols	2470:2500	This paper specifically reports the discovery of optimal VH extraction protocols and cell seeding strategy enabling fabrication of cartilaginous matrix components into a hydrogel support material for promoting chondrogenic differentiation.
30572166	11	52	theme	alternative	2311:2321	arg1	route					2323:2327	an alternative route	2308:2327	an alternative route to fabricate highly permissive and bioactive ECM hydrogels from vitreous humor (VH) tissue	2308:2418	In this study, we present an alternative route to fabricate highly permissive and bioactive ECM hydrogels from vitreous humor (VH) tissue.
30572166	13	53	dep	instructive	2806:2816	arg1	biomaterials					2848:2859	responsive hybrid biomaterials	2830:2859	responsive hybrid biomaterials	2830:2859	The work showcases a naturally intact and unmodified hydrogel design that improves cellular responses and may help guide the development of cell instructive and stimuli responsive hybrid biomaterials in a number of TERM applications.
30572166	4	54	theme	Equine	707:712	arg1	VH					714:715	Equine VH	707:715	Equine VH	707:715	Equine VH was extracted followed by biochemical quantifications, histological examinations, cytotoxicity (human mesenchymal stromal cells, hMSCs and human articular chondrocytes, hACs) and U937 cell proliferation studies.
30572166	2	55	theme	cellular	413:420	arg1	ECM					430:432	ECM	430:432	ECM	430:432	However, removing DNA from cartilage (dCart) requires harsh treatments due to its dense structure, causing loss of bioactivity and limiting its application as a cartilaginous extra cellular matrix (ECM).
30572166	2	55	theme	cellular	413:420	arg1	matrix					422:427	a cartilaginous extra cellular matrix	391:427	a cartilaginous extra cellular matrix (ECM)	391:433	However, removing DNA from cartilage (dCart) requires harsh treatments due to its dense structure, causing loss of bioactivity and limiting its application as a cartilaginous extra cellular matrix (ECM).
30572166	9	56	theme	native	1854:1859	arg1	source					1865:1870	a permissive native ECM source	1841:1870	VH a permissive native ECM source	1838:1870	Interestingly, encapsulated cells self-assembled the VH-hydrogel into spheroids, resulting in uniform distribution of both GAGs and collagen type II with increased compressive mechanical properties, rendering VH a permissive native ECM source to fabricate cartilaginous hydrogels for potential TE applications.
30572166	8	57	theme	U937	1566:1569	arg1	cells					1571:1575	macrophage-like U937 cells	1550:1575	macrophage-like U937 cells	1550:1575	VH displayed neither cytotoxic responses nor proliferation of macrophage-like U937 cells, instead enhancing both hMSC and hAC proliferation.
30572166	2	58	theme	cartilaginous	393:405	arg1	ECM					430:432	ECM	430:432	ECM	430:432	However, removing DNA from cartilage (dCart) requires harsh treatments due to its dense structure, causing loss of bioactivity and limiting its application as a cartilaginous extra cellular matrix (ECM).
30572166	2	58	theme	cartilaginous	393:405	arg1	matrix					422:427	a cartilaginous extra cellular matrix	391:427	a cartilaginous extra cellular matrix (ECM)	391:433	However, removing DNA from cartilage (dCart) requires harsh treatments due to its dense structure, causing loss of bioactivity and limiting its application as a cartilaginous extra cellular matrix (ECM).
30572166	10	59	theme	bioactive	1979:1987	arg1	matrix					2034:2039	Fabricating bioactive and cell-instructive cartilage extracellular matrix	1967:2039	SIGNIFICANCE: Fabricating bioactive and cell-instructive cartilage extracellular matrix (ECM)	1953:2045	STATEMENT OF SIGNIFICANCE: Fabricating bioactive and cell-instructive cartilage extracellular matrix (ECM) derived biomaterials and hydrogels has over recent years proven to be a challenging task, often limited by poor retention of inherent environmental cues post decellularisation due to the dense and avascular nature of native cartilage.
30572166	10	59	theme	bioactive	1979:1987	arg1	ECM					2042:2044	ECM	2042:2044	ECM	2042:2044	STATEMENT OF SIGNIFICANCE: Fabricating bioactive and cell-instructive cartilage extracellular matrix (ECM) derived biomaterials and hydrogels has over recent years proven to be a challenging task, often limited by poor retention of inherent environmental cues post decellularisation due to the dense and avascular nature of native cartilage.
30572166	4	60	dep	cells	839:843	arg1	hACs					886:889	hACs	886:889	hACs	886:889	Equine VH was extracted followed by biochemical quantifications, histological examinations, cytotoxicity (human mesenchymal stromal cells, hMSCs and human articular chondrocytes, hACs) and U937 cell proliferation studies.
30572166	11	61	theme	ECM	2374:2376	arg1	hydrogels					2378:2386	highly permissive and bioactive ECM hydrogels	2342:2386	highly permissive and bioactive ECM hydrogels	2342:2386	In this study, we present an alternative route to fabricate highly permissive and bioactive ECM hydrogels from vitreous humor (VH) tissue.
30572166	10	62	dep	SIGNIFICANCE	1953:1964	arg1	matrix					2034:2039	Fabricating bioactive and cell-instructive cartilage extracellular matrix	1967:2039	SIGNIFICANCE: Fabricating bioactive and cell-instructive cartilage extracellular matrix (ECM)	1953:2045	STATEMENT OF SIGNIFICANCE: Fabricating bioactive and cell-instructive cartilage extracellular matrix (ECM) derived biomaterials and hydrogels has over recent years proven to be a challenging task, often limited by poor retention of inherent environmental cues post decellularisation due to the dense and avascular nature of native cartilage.
30572166	10	62	dep	SIGNIFICANCE	1953:1964	arg1	ECM					2042:2044	ECM	2042:2044	ECM	2042:2044	STATEMENT OF SIGNIFICANCE: Fabricating bioactive and cell-instructive cartilage extracellular matrix (ECM) derived biomaterials and hydrogels has over recent years proven to be a challenging task, often limited by poor retention of inherent environmental cues post decellularisation due to the dense and avascular nature of native cartilage.
30572166	4	63	theme	biochemical	743:753	arg1	quantifications					755:769	biochemical quantifications	743:769	biochemical quantifications	743:769	Equine VH was extracted followed by biochemical quantifications, histological examinations, cytotoxicity (human mesenchymal stromal cells, hMSCs and human articular chondrocytes, hACs) and U937 cell proliferation studies.
30572166	12	64	theme	cartilaginous	2552:2564	arg1	components					2573:2582	cartilaginous matrix components	2552:2582	cartilaginous matrix components	2552:2582	This paper specifically reports the discovery of optimal VH extraction protocols and cell seeding strategy enabling fabrication of cartilaginous matrix components into a hydrogel support material for promoting chondrogenic differentiation.
30572166	7	65	theme	DNA	1411:1413	arg1	DNA					1411:1413	DNA	1411:1413	DNA (0.4 ± 0.4 µg/mg dry-weight)	1411:1442	VH was successfully extracted, exhibiting negligible amounts of DNA (0.4 ± 0.4 µg/mg dry-weight) and notable preservation of ECM components.
30572166	7	65	theme	DNA	1411:1413	arg1	dry-weight					1432:1441	0.4 ± 0.4 µg/mg dry-weight	1416:1441	0.4 ± 0.4 µg/mg dry-weight	1416:1441	VH was successfully extracted, exhibiting negligible amounts of DNA (0.4 ± 0.4 µg/mg dry-weight) and notable preservation of ECM components.
30572166	7	65	theme	DNA	1411:1413	arg1	amounts					1400:1406	negligible amounts	1389:1406	negligible amounts of DNA (0.4 ± 0.4 µg/mg dry-weight)	1389:1442	VH was successfully extracted, exhibiting negligible amounts of DNA (0.4 ± 0.4 µg/mg dry-weight) and notable preservation of ECM components.
30572166	7	65	theme	DNA	1411:1413	arg1	preservation					1456:1467	notable preservation	1448:1467	notable preservation of ECM components	1448:1485	VH was successfully extracted, exhibiting negligible amounts of DNA (0.4 ± 0.4 µg/mg dry-weight) and notable preservation of ECM components.
30572166	7	65	theme	DNA	1411:1413	arg1	components					1476:1485	ECM components	1472:1485	ECM components	1472:1485	VH was successfully extracted, exhibiting negligible amounts of DNA (0.4 ± 0.4 µg/mg dry-weight) and notable preservation of ECM components.
30572166	0	66	dep	humor	16:20	arg1	hydrogel					58:65	a potential extracellular matrix hydrogel	25:65	a potential extracellular matrix hydrogel for cartilage tissue engineering applications	25:111	Intact vitreous humor as a potential extracellular matrix hydrogel for cartilage tissue engineering applications.
30572166	1	67	theme	biochemical	160:170	arg1	cues					172:175	their biochemical cues	154:175	their biochemical cues	154:175	Decellularisation of tissues, utilising their biochemical cues, poses exciting tissue engineering (TE) opportunities.
30572166	3	68	theme	collagen	612:619	arg1	composition					621:631	collagen composition	612:631	collagen composition of cartilage	612:644	In this study, we demonstrate for the first time the successful application of vitreous humor (VH), a highly hydrated tissue closely resembling the glycosaminoglycan (GAG) and collagen composition of cartilage, as an ECM hydrogel to support chondrogenic differentiation.
30572166	7	69	theme	ECM	1472:1474	arg1	components					1476:1485	ECM components	1472:1485	ECM components	1472:1485	VH was successfully extracted, exhibiting negligible amounts of DNA (0.4 ± 0.4 µg/mg dry-weight) and notable preservation of ECM components.
30572166	9	70	theme	encapsulated	1644:1655	arg1	cells					1657:1661	encapsulated cells	1644:1661	encapsulated cells	1644:1661	Interestingly, encapsulated cells self-assembled the VH-hydrogel into spheroids, resulting in uniform distribution of both GAGs and collagen type II with increased compressive mechanical properties, rendering VH a permissive native ECM source to fabricate cartilaginous hydrogels for potential TE applications.
30572166	9	71	theme	potential	1913:1921	arg1	applications					1926:1937	potential TE applications	1913:1937	potential TE applications	1913:1937	Interestingly, encapsulated cells self-assembled the VH-hydrogel into spheroids, resulting in uniform distribution of both GAGs and collagen type II with increased compressive mechanical properties, rendering VH a permissive native ECM source to fabricate cartilaginous hydrogels for potential TE applications.
30572166	8	72	theme	hAC	1610:1612	arg1	proliferation					1614:1626	hAC proliferation	1610:1626	hAC proliferation	1610:1626	VH displayed neither cytotoxic responses nor proliferation of macrophage-like U937 cells, instead enhancing both hMSC and hAC proliferation.
30572166	6	73	theme	matrix	1258:1263	arg1	deposition					1265:1274	matrix deposition	1258:1274	matrix deposition	1258:1274	Viability, metabolic activity, GAG and DNA content, chondrogenic gene expression (aggrecan, collagen I/II mRNA) and mechanical properties were quantified and matrix deposition was visualised using immunohistochemistry (Safranin-O, collagen I/II).
30572166	13	74	theme	instructive	2806:2816	arg1	development					2786:2796	the development	2782:2796	the development of cell instructive and stimuli responsive hybrid biomaterials in a number of TERM applications	2782:2892	The work showcases a naturally intact and unmodified hydrogel design that improves cellular responses and may help guide the development of cell instructive and stimuli responsive hybrid biomaterials in a number of TERM applications.
30572166	12	75	theme	hydrogel	2591:2598	arg1	material					2608:2615	a hydrogel support material	2589:2615	a hydrogel support material for promoting chondrogenic differentiation	2589:2658	This paper specifically reports the discovery of optimal VH extraction protocols and cell seeding strategy enabling fabrication of cartilaginous matrix components into a hydrogel support material for promoting chondrogenic differentiation.
30572166	2	76	theme	harsh	286:290	arg1	treatments					292:301	harsh treatments	286:301	harsh treatments	286:301	However, removing DNA from cartilage (dCart) requires harsh treatments due to its dense structure, causing loss of bioactivity and limiting its application as a cartilaginous extra cellular matrix (ECM).
30572166	13	77	theme	stimuli	2822:2828	arg1	development					2786:2796	the development	2782:2796	the development of cell instructive and stimuli responsive hybrid biomaterials in a number of TERM applications	2782:2892	The work showcases a naturally intact and unmodified hydrogel design that improves cellular responses and may help guide the development of cell instructive and stimuli responsive hybrid biomaterials in a number of TERM applications.
30572166	7	78	theme	0.4 ± 0.4 µg/mg	1416:1430	arg1	DNA					1411:1413	DNA	1411:1413	DNA (0.4 ± 0.4 µg/mg dry-weight)	1411:1442	VH was successfully extracted, exhibiting negligible amounts of DNA (0.4 ± 0.4 µg/mg dry-weight) and notable preservation of ECM components.
30572166	7	78	theme	0.4 ± 0.4 µg/mg	1416:1430	arg1	dry-weight					1432:1441	0.4 ± 0.4 µg/mg dry-weight	1416:1441	0.4 ± 0.4 µg/mg dry-weight	1416:1441	VH was successfully extracted, exhibiting negligible amounts of DNA (0.4 ± 0.4 µg/mg dry-weight) and notable preservation of ECM components.
30572166	10	79	theme	inherent	2172:2179	arg1	cues					2195:2198	inherent environmental cues	2172:2198	inherent environmental cues	2172:2198	STATEMENT OF SIGNIFICANCE: Fabricating bioactive and cell-instructive cartilage extracellular matrix (ECM) derived biomaterials and hydrogels has over recent years proven to be a challenging task, often limited by poor retention of inherent environmental cues post decellularisation due to the dense and avascular nature of native cartilage.
30572166	10	80	theme	poor	2154:2157	arg1	retention					2159:2167	poor retention	2154:2167	poor retention of inherent environmental cues post decellularisation due to the dense and avascular nature of native cartilage	2154:2279	STATEMENT OF SIGNIFICANCE: Fabricating bioactive and cell-instructive cartilage extracellular matrix (ECM) derived biomaterials and hydrogels has over recent years proven to be a challenging task, often limited by poor retention of inherent environmental cues post decellularisation due to the dense and avascular nature of native cartilage.
30572166	3	81	theme	successful	489:498	arg1	hydrogel					657:664	an ECM hydrogel	650:664	an ECM hydrogel to support chondrogenic differentiation	650:704	In this study, we demonstrate for the first time the successful application of vitreous humor (VH), a highly hydrated tissue closely resembling the glycosaminoglycan (GAG) and collagen composition of cartilage, as an ECM hydrogel to support chondrogenic differentiation.
30572166	3	81	theme	successful	489:498	arg1	application					500:510	the successful application	485:510	the successful application of vitreous humor (VH), a highly hydrated tissue closely resembling the glycosaminoglycan (GAG) and collagen composition of cartilage,	485:645	In this study, we demonstrate for the first time the successful application of vitreous humor (VH), a highly hydrated tissue closely resembling the glycosaminoglycan (GAG) and collagen composition of cartilage, as an ECM hydrogel to support chondrogenic differentiation.
30572166	0	82	theme	extracellular	37:49	arg1	hydrogel					58:65	a potential extracellular matrix hydrogel	25:65	a potential extracellular matrix hydrogel for cartilage tissue engineering applications	25:111	Intact vitreous humor as a potential extracellular matrix hydrogel for cartilage tissue engineering applications.
30572166	6	83	theme	DNA	1139:1141	arg1	content					1143:1149	GAG and DNA content	1131:1149	content	1143:1149	Viability, metabolic activity, GAG and DNA content, chondrogenic gene expression (aggrecan, collagen I/II mRNA) and mechanical properties were quantified and matrix deposition was visualised using immunohistochemistry (Safranin-O, collagen I/II).
30572166	12	84	theme	matrix	2566:2571	arg1	components					2573:2582	cartilaginous matrix components	2552:2582	cartilaginous matrix components	2552:2582	This paper specifically reports the discovery of optimal VH extraction protocols and cell seeding strategy enabling fabrication of cartilaginous matrix components into a hydrogel support material for promoting chondrogenic differentiation.
30572166	10	85	theme	Fabricating	1967:1977	arg1	matrix					2034:2039	Fabricating bioactive and cell-instructive cartilage extracellular matrix	1967:2039	SIGNIFICANCE: Fabricating bioactive and cell-instructive cartilage extracellular matrix (ECM)	1953:2045	STATEMENT OF SIGNIFICANCE: Fabricating bioactive and cell-instructive cartilage extracellular matrix (ECM) derived biomaterials and hydrogels has over recent years proven to be a challenging task, often limited by poor retention of inherent environmental cues post decellularisation due to the dense and avascular nature of native cartilage.
30572166	10	85	theme	Fabricating	1967:1977	arg1	ECM					2042:2044	ECM	2042:2044	ECM	2042:2044	STATEMENT OF SIGNIFICANCE: Fabricating bioactive and cell-instructive cartilage extracellular matrix (ECM) derived biomaterials and hydrogels has over recent years proven to be a challenging task, often limited by poor retention of inherent environmental cues post decellularisation due to the dense and avascular nature of native cartilage.
30572166	1	86	theme	tissue	193:198	arg1	TE					213:214	TE	213:214	TE	213:214	Decellularisation of tissues, utilising their biochemical cues, poses exciting tissue engineering (TE) opportunities.
30572166	1	86	theme	tissue	193:198	arg1	engineering					200:210	tissue engineering	193:210	exciting tissue engineering (TE) opportunities	184:229	Decellularisation of tissues, utilising their biochemical cues, poses exciting tissue engineering (TE) opportunities.
30572166	4	87	theme	mesenchymal	819:829	arg1	chondrocytes					872:883	human articular chondrocytes	856:883	human articular chondrocytes	856:883	Equine VH was extracted followed by biochemical quantifications, histological examinations, cytotoxicity (human mesenchymal stromal cells, hMSCs and human articular chondrocytes, hACs) and U937 cell proliferation studies.
30572166	4	87	theme	mesenchymal	819:829	arg1	hMSCs					846:850	hMSCs	846:850	hMSCs	846:850	Equine VH was extracted followed by biochemical quantifications, histological examinations, cytotoxicity (human mesenchymal stromal cells, hMSCs and human articular chondrocytes, hACs) and U937 cell proliferation studies.
30572166	4	87	theme	mesenchymal	819:829	arg1	cells					839:843	human mesenchymal stromal cells	813:843	human mesenchymal stromal cells	813:843	Equine VH was extracted followed by biochemical quantifications, histological examinations, cytotoxicity (human mesenchymal stromal cells, hMSCs and human articular chondrocytes, hACs) and U937 cell proliferation studies.
30572166	4	87	theme	mesenchymal	819:829	arg1	cytotoxicity					799:810	cytotoxicity	799:810	cytotoxicity (human mesenchymal stromal cells, hMSCs and human articular chondrocytes, hACs)	799:890	Equine VH was extracted followed by biochemical quantifications, histological examinations, cytotoxicity (human mesenchymal stromal cells, hMSCs and human articular chondrocytes, hACs) and U937 cell proliferation studies.
30572166	6	88	theme	GAG	1131:1133	arg1	content					1143:1149	GAG and DNA content	1131:1149	content	1143:1149	Viability, metabolic activity, GAG and DNA content, chondrogenic gene expression (aggrecan, collagen I/II mRNA) and mechanical properties were quantified and matrix deposition was visualised using immunohistochemistry (Safranin-O, collagen I/II).
30572166	3	89	theme	humor	524:528	arg1	hydrogel					657:664	an ECM hydrogel	650:664	an ECM hydrogel to support chondrogenic differentiation	650:704	In this study, we demonstrate for the first time the successful application of vitreous humor (VH), a highly hydrated tissue closely resembling the glycosaminoglycan (GAG) and collagen composition of cartilage, as an ECM hydrogel to support chondrogenic differentiation.
30572166	3	89	theme	humor	524:528	arg1	application					500:510	the successful application	485:510	the successful application of vitreous humor (VH), a highly hydrated tissue closely resembling the glycosaminoglycan (GAG) and collagen composition of cartilage,	485:645	In this study, we demonstrate for the first time the successful application of vitreous humor (VH), a highly hydrated tissue closely resembling the glycosaminoglycan (GAG) and collagen composition of cartilage, as an ECM hydrogel to support chondrogenic differentiation.
30572166	9	90	theme	uniform	1723:1729	arg1	distribution					1731:1742	uniform distribution	1723:1742	uniform distribution of both GAGs and collagen type II	1723:1776	Interestingly, encapsulated cells self-assembled the VH-hydrogel into spheroids, resulting in uniform distribution of both GAGs and collagen type II with increased compressive mechanical properties, rendering VH a permissive native ECM source to fabricate cartilaginous hydrogels for potential TE applications.
30572166	10	91	theme	cues	2195:2198	arg1	retention					2159:2167	poor retention	2154:2167	poor retention of inherent environmental cues post decellularisation due to the dense and avascular nature of native cartilage	2154:2279	STATEMENT OF SIGNIFICANCE: Fabricating bioactive and cell-instructive cartilage extracellular matrix (ECM) derived biomaterials and hydrogels has over recent years proven to be a challenging task, often limited by poor retention of inherent environmental cues post decellularisation due to the dense and avascular nature of native cartilage.
30572166	10	92	theme	cartilage	2010:2018	arg1	matrix					2034:2039	Fabricating bioactive and cell-instructive cartilage extracellular matrix	1967:2039	SIGNIFICANCE: Fabricating bioactive and cell-instructive cartilage extracellular matrix (ECM)	1953:2045	STATEMENT OF SIGNIFICANCE: Fabricating bioactive and cell-instructive cartilage extracellular matrix (ECM) derived biomaterials and hydrogels has over recent years proven to be a challenging task, often limited by poor retention of inherent environmental cues post decellularisation due to the dense and avascular nature of native cartilage.
30572166	10	92	theme	cartilage	2010:2018	arg1	ECM					2042:2044	ECM	2042:2044	ECM	2042:2044	STATEMENT OF SIGNIFICANCE: Fabricating bioactive and cell-instructive cartilage extracellular matrix (ECM) derived biomaterials and hydrogels has over recent years proven to be a challenging task, often limited by poor retention of inherent environmental cues post decellularisation due to the dense and avascular nature of native cartilage.
30572166	5	93	theme	scaffold-free	1031:1043	arg1	cultures					1065:1072	scaffold-free micro-tissue pellet cultures	1031:1072	scaffold-free micro-tissue pellet cultures	1031:1072	VH was further seeded with hACs or hMSCs and cultured for 3-weeks to study chondrogenesis compared to scaffold-free micro-tissue pellet cultures and collagen-I hydrogels.
30572166	6	94	dep	immunohistochemistry	1297:1316	arg1	Safranin-O					1319:1328	Safranin-O	1319:1328	Safranin-O	1319:1328	Viability, metabolic activity, GAG and DNA content, chondrogenic gene expression (aggrecan, collagen I/II mRNA) and mechanical properties were quantified and matrix deposition was visualised using immunohistochemistry (Safranin-O, collagen I/II).
30572166	6	94	dep	immunohistochemistry	1297:1316	arg1	I/II					1340:1343	collagen I/II	1331:1343	collagen I/II	1331:1343	Viability, metabolic activity, GAG and DNA content, chondrogenic gene expression (aggrecan, collagen I/II mRNA) and mechanical properties were quantified and matrix deposition was visualised using immunohistochemistry (Safranin-O, collagen I/II).
30572166	6	95	theme	I/II	1201:1204	arg1	mRNA					1206:1209	collagen I/II mRNA	1192:1209	collagen I/II mRNA	1192:1209	Viability, metabolic activity, GAG and DNA content, chondrogenic gene expression (aggrecan, collagen I/II mRNA) and mechanical properties were quantified and matrix deposition was visualised using immunohistochemistry (Safranin-O, collagen I/II).
30572166	10	96	theme	dense	2234:2238	arg1	nature					2254:2259	the dense and avascular nature	2230:2259	the dense and avascular nature of native cartilage	2230:2279	STATEMENT OF SIGNIFICANCE: Fabricating bioactive and cell-instructive cartilage extracellular matrix (ECM) derived biomaterials and hydrogels has over recent years proven to be a challenging task, often limited by poor retention of inherent environmental cues post decellularisation due to the dense and avascular nature of native cartilage.
30572166	9	97	theme	compressive	1793:1803	arg1	properties					1816:1825	increased compressive mechanical properties	1783:1825	increased compressive mechanical properties	1783:1825	Interestingly, encapsulated cells self-assembled the VH-hydrogel into spheroids, resulting in uniform distribution of both GAGs and collagen type II with increased compressive mechanical properties, rendering VH a permissive native ECM source to fabricate cartilaginous hydrogels for potential TE applications.
30572166	5	98	theme	pellet	1058:1063	arg1	cultures					1065:1072	scaffold-free micro-tissue pellet cultures	1031:1072	scaffold-free micro-tissue pellet cultures	1031:1072	VH was further seeded with hACs or hMSCs and cultured for 3-weeks to study chondrogenesis compared to scaffold-free micro-tissue pellet cultures and collagen-I hydrogels.
30572166	4	99	theme	human	856:860	arg1	chondrocytes					872:883	human articular chondrocytes	856:883	human articular chondrocytes	856:883	Equine VH was extracted followed by biochemical quantifications, histological examinations, cytotoxicity (human mesenchymal stromal cells, hMSCs and human articular chondrocytes, hACs) and U937 cell proliferation studies.
30572166	4	99	theme	human	856:860	arg1	cells					839:843	human mesenchymal stromal cells	813:843	human mesenchymal stromal cells	813:843	Equine VH was extracted followed by biochemical quantifications, histological examinations, cytotoxicity (human mesenchymal stromal cells, hMSCs and human articular chondrocytes, hACs) and U937 cell proliferation studies.
30572166	9	100	theme	collagen	1761:1768	arg1	type					1770:1773	collagen type II	1761:1776	collagen type II	1761:1776	Interestingly, encapsulated cells self-assembled the VH-hydrogel into spheroids, resulting in uniform distribution of both GAGs and collagen type II with increased compressive mechanical properties, rendering VH a permissive native ECM source to fabricate cartilaginous hydrogels for potential TE applications.
30572166	12	101	theme	support	2600:2606	arg1	material					2608:2615	a hydrogel support material	2589:2615	a hydrogel support material for promoting chondrogenic differentiation	2589:2658	This paper specifically reports the discovery of optimal VH extraction protocols and cell seeding strategy enabling fabrication of cartilaginous matrix components into a hydrogel support material for promoting chondrogenic differentiation.
30572166	12	102	theme	cell	2506:2509	arg1	seeding					2511:2517	cell seeding	2506:2517	cell seeding	2506:2517	This paper specifically reports the discovery of optimal VH extraction protocols and cell seeding strategy enabling fabrication of cartilaginous matrix components into a hydrogel support material for promoting chondrogenic differentiation.
30572166	10	103	theme	challenging	2119:2129	arg1	STATEMENT					1940:1948	STATEMENT	1940:1948	STATEMENT OF SIGNIFICANCE: Fabricating bioactive and cell-instructive cartilage extracellular matrix (ECM) derived biomaterials and hydrogels	1940:2080	STATEMENT OF SIGNIFICANCE: Fabricating bioactive and cell-instructive cartilage extracellular matrix (ECM) derived biomaterials and hydrogels has over recent years proven to be a challenging task, often limited by poor retention of inherent environmental cues post decellularisation due to the dense and avascular nature of native cartilage.
30572166	10	103	theme	challenging	2119:2129	arg1	task					2131:2134	a challenging task	2117:2134	a challenging task	2117:2134	STATEMENT OF SIGNIFICANCE: Fabricating bioactive and cell-instructive cartilage extracellular matrix (ECM) derived biomaterials and hydrogels has over recent years proven to be a challenging task, often limited by poor retention of inherent environmental cues post decellularisation due to the dense and avascular nature of native cartilage.
30572166	6	104	theme	gene	1165:1168	arg1	aggrecan					1182:1189	aggrecan	1182:1189	aggrecan	1182:1189	Viability, metabolic activity, GAG and DNA content, chondrogenic gene expression (aggrecan, collagen I/II mRNA) and mechanical properties were quantified and matrix deposition was visualised using immunohistochemistry (Safranin-O, collagen I/II).
30572166	6	104	theme	gene	1165:1168	arg1	expression					1170:1179	chondrogenic gene expression	1152:1179	chondrogenic gene expression (aggrecan, collagen I/II mRNA)	1152:1210	Viability, metabolic activity, GAG and DNA content, chondrogenic gene expression (aggrecan, collagen I/II mRNA) and mechanical properties were quantified and matrix deposition was visualised using immunohistochemistry (Safranin-O, collagen I/II).
30572166	10	105	theme	avascular	2244:2252	arg1	nature					2254:2259	the dense and avascular nature	2230:2259	the dense and avascular nature of native cartilage	2230:2279	STATEMENT OF SIGNIFICANCE: Fabricating bioactive and cell-instructive cartilage extracellular matrix (ECM) derived biomaterials and hydrogels has over recent years proven to be a challenging task, often limited by poor retention of inherent environmental cues post decellularisation due to the dense and avascular nature of native cartilage.
30572166	4	106	theme	cell	901:904	arg1	studies					920:926	U937 cell proliferation studies	896:926	U937 cell proliferation studies	896:926	Equine VH was extracted followed by biochemical quantifications, histological examinations, cytotoxicity (human mesenchymal stromal cells, hMSCs and human articular chondrocytes, hACs) and U937 cell proliferation studies.
30572166	0	107	theme	tissue	81:86	arg1	applications					100:111	cartilage tissue engineering applications	71:111	cartilage tissue engineering applications	71:111	Intact vitreous humor as a potential extracellular matrix hydrogel for cartilage tissue engineering applications.
30572166	10	108	theme	extracellular	2020:2032	arg1	matrix					2034:2039	Fabricating bioactive and cell-instructive cartilage extracellular matrix	1967:2039	SIGNIFICANCE: Fabricating bioactive and cell-instructive cartilage extracellular matrix (ECM)	1953:2045	STATEMENT OF SIGNIFICANCE: Fabricating bioactive and cell-instructive cartilage extracellular matrix (ECM) derived biomaterials and hydrogels has over recent years proven to be a challenging task, often limited by poor retention of inherent environmental cues post decellularisation due to the dense and avascular nature of native cartilage.
30572166	10	108	theme	extracellular	2020:2032	arg1	ECM					2042:2044	ECM	2042:2044	ECM	2042:2044	STATEMENT OF SIGNIFICANCE: Fabricating bioactive and cell-instructive cartilage extracellular matrix (ECM) derived biomaterials and hydrogels has over recent years proven to be a challenging task, often limited by poor retention of inherent environmental cues post decellularisation due to the dense and avascular nature of native cartilage.
30572166	9	109	theme	increased	1783:1791	arg1	properties					1816:1825	increased compressive mechanical properties	1783:1825	increased compressive mechanical properties	1783:1825	Interestingly, encapsulated cells self-assembled the VH-hydrogel into spheroids, resulting in uniform distribution of both GAGs and collagen type II with increased compressive mechanical properties, rendering VH a permissive native ECM source to fabricate cartilaginous hydrogels for potential TE applications.
30572166	2	110	dep	requires	277:284	arg1	limiting					363:370	limiting	363:370	limiting its application as a cartilaginous extra cellular matrix (ECM)	363:433	However, removing DNA from cartilage (dCart) requires harsh treatments due to its dense structure, causing loss of bioactivity and limiting its application as a cartilaginous extra cellular matrix (ECM).
30572166	2	110	dep	requires	277:284	arg1	causing					331:337	causing	331:337	causing loss of bioactivity	331:357	However, removing DNA from cartilage (dCart) requires harsh treatments due to its dense structure, causing loss of bioactivity and limiting its application as a cartilaginous extra cellular matrix (ECM).
30572166	13	111	theme	intact	2692:2697	arg1	design					2723:2728	a naturally intact and unmodified hydrogel design	2680:2728	a naturally intact and unmodified hydrogel design that improves cellular responses and may help guide the development of cell instructive and stimuli responsive hybrid biomaterials in a number of TERM applications	2680:2892	The work showcases a naturally intact and unmodified hydrogel design that improves cellular responses and may help guide the development of cell instructive and stimuli responsive hybrid biomaterials in a number of TERM applications.
30572166	9	112	theme	mechanical	1805:1814	arg1	properties					1816:1825	increased compressive mechanical properties	1783:1825	increased compressive mechanical properties	1783:1825	Interestingly, encapsulated cells self-assembled the VH-hydrogel into spheroids, resulting in uniform distribution of both GAGs and collagen type II with increased compressive mechanical properties, rendering VH a permissive native ECM source to fabricate cartilaginous hydrogels for potential TE applications.
30572166	10	113	theme	cartilage	2271:2279	arg1	nature					2254:2259	the dense and avascular nature	2230:2259	the dense and avascular nature of native cartilage	2230:2279	STATEMENT OF SIGNIFICANCE: Fabricating bioactive and cell-instructive cartilage extracellular matrix (ECM) derived biomaterials and hydrogels has over recent years proven to be a challenging task, often limited by poor retention of inherent environmental cues post decellularisation due to the dense and avascular nature of native cartilage.
30572166	10	114	theme	recent	2091:2096	arg1	years					2098:2102	recent years	2091:2102	recent years	2091:2102	STATEMENT OF SIGNIFICANCE: Fabricating bioactive and cell-instructive cartilage extracellular matrix (ECM) derived biomaterials and hydrogels has over recent years proven to be a challenging task, often limited by poor retention of inherent environmental cues post decellularisation due to the dense and avascular nature of native cartilage.
30572166	13	115	theme	unmodified	2703:2712	arg1	design					2723:2728	a naturally intact and unmodified hydrogel design	2680:2728	a naturally intact and unmodified hydrogel design that improves cellular responses and may help guide the development of cell instructive and stimuli responsive hybrid biomaterials in a number of TERM applications	2680:2892	The work showcases a naturally intact and unmodified hydrogel design that improves cellular responses and may help guide the development of cell instructive and stimuli responsive hybrid biomaterials in a number of TERM applications.
30572166	0	116	theme	vitreous	7:14	arg1	humor					16:20	Intact vitreous humor	0:20	Intact vitreous humor	0:20	Intact vitreous humor as a potential extracellular matrix hydrogel for cartilage tissue engineering applications.
30572166	13	117	theme	cellular	2744:2751	arg1	responses					2753:2761	cellular responses	2744:2761	cellular responses	2744:2761	The work showcases a naturally intact and unmodified hydrogel design that improves cellular responses and may help guide the development of cell instructive and stimuli responsive hybrid biomaterials in a number of TERM applications.
30572166	8	118	theme	macrophage-like	1550:1564	arg1	cells					1571:1575	macrophage-like U937 cells	1550:1575	macrophage-like U937 cells	1550:1575	VH displayed neither cytotoxic responses nor proliferation of macrophage-like U937 cells, instead enhancing both hMSC and hAC proliferation.
30572166	13	119	theme	TERM	2876:2879	arg1	applications					2881:2892	TERM applications	2876:2892	TERM applications	2876:2892	The work showcases a naturally intact and unmodified hydrogel design that improves cellular responses and may help guide the development of cell instructive and stimuli responsive hybrid biomaterials in a number of TERM applications.
30572166	9	120	theme	permissive	1843:1852	arg1	source					1865:1870	a permissive native ECM source	1841:1870	VH a permissive native ECM source	1838:1870	Interestingly, encapsulated cells self-assembled the VH-hydrogel into spheroids, resulting in uniform distribution of both GAGs and collagen type II with increased compressive mechanical properties, rendering VH a permissive native ECM source to fabricate cartilaginous hydrogels for potential TE applications.
30572166	8	121	theme	cells	1571:1575	arg1	proliferation					1533:1545	proliferation	1533:1545	proliferation of macrophage-like U937 cells	1533:1575	VH displayed neither cytotoxic responses nor proliferation of macrophage-like U937 cells, instead enhancing both hMSC and hAC proliferation.
30572166	2	122	theme	extra	407:411	arg1	ECM					430:432	ECM	430:432	ECM	430:432	However, removing DNA from cartilage (dCart) requires harsh treatments due to its dense structure, causing loss of bioactivity and limiting its application as a cartilaginous extra cellular matrix (ECM).
30572166	2	122	theme	extra	407:411	arg1	matrix					422:427	a cartilaginous extra cellular matrix	391:427	a cartilaginous extra cellular matrix (ECM)	391:433	However, removing DNA from cartilage (dCart) requires harsh treatments due to its dense structure, causing loss of bioactivity and limiting its application as a cartilaginous extra cellular matrix (ECM).
30572166	0	123	theme	potential	27:35	arg1	hydrogel					58:65	a potential extracellular matrix hydrogel	25:65	a potential extracellular matrix hydrogel for cartilage tissue engineering applications	25:111	Intact vitreous humor as a potential extracellular matrix hydrogel for cartilage tissue engineering applications.
30572166	9	124	theme	ECM	1861:1863	arg1	source					1865:1870	a permissive native ECM source	1841:1870	VH a permissive native ECM source	1838:1870	Interestingly, encapsulated cells self-assembled the VH-hydrogel into spheroids, resulting in uniform distribution of both GAGs and collagen type II with increased compressive mechanical properties, rendering VH a permissive native ECM source to fabricate cartilaginous hydrogels for potential TE applications.
30572166	4	125	theme	histological	772:783	arg1	examinations					785:796	histological examinations	772:796	histological examinations	772:796	Equine VH was extracted followed by biochemical quantifications, histological examinations, cytotoxicity (human mesenchymal stromal cells, hMSCs and human articular chondrocytes, hACs) and U937 cell proliferation studies.
30572166	13	126	theme	hydrogel	2714:2721	arg1	design					2723:2728	a naturally intact and unmodified hydrogel design	2680:2728	a naturally intact and unmodified hydrogel design that improves cellular responses and may help guide the development of cell instructive and stimuli responsive hybrid biomaterials in a number of TERM applications	2680:2892	The work showcases a naturally intact and unmodified hydrogel design that improves cellular responses and may help guide the development of cell instructive and stimuli responsive hybrid biomaterials in a number of TERM applications.
30572166	0	127	theme	matrix	51:56	arg1	hydrogel					58:65	a potential extracellular matrix hydrogel	25:65	a potential extracellular matrix hydrogel for cartilage tissue engineering applications	25:111	Intact vitreous humor as a potential extracellular matrix hydrogel for cartilage tissue engineering applications.
30572166	6	128	theme	metabolic	1111:1119	arg1	activity					1121:1128	metabolic activity	1111:1128	metabolic activity	1111:1128	Viability, metabolic activity, GAG and DNA content, chondrogenic gene expression (aggrecan, collagen I/II mRNA) and mechanical properties were quantified and matrix deposition was visualised using immunohistochemistry (Safranin-O, collagen I/II).
30572166	12	129	theme	optimal	2470:2476	arg1	protocols					2492:2500	optimal VH extraction protocols	2470:2500	optimal VH extraction protocols	2470:2500	This paper specifically reports the discovery of optimal VH extraction protocols and cell seeding strategy enabling fabrication of cartilaginous matrix components into a hydrogel support material for promoting chondrogenic differentiation.
30572166	13	130	theme	responsive	2830:2839	arg1	biomaterials					2848:2859	responsive hybrid biomaterials	2830:2859	responsive hybrid biomaterials	2830:2859	The work showcases a naturally intact and unmodified hydrogel design that improves cellular responses and may help guide the development of cell instructive and stimuli responsive hybrid biomaterials in a number of TERM applications.
30572166	9	131	theme	cartilaginous	1885:1897	arg1	hydrogels					1899:1907	cartilaginous hydrogels	1885:1907	cartilaginous hydrogels for potential TE applications	1885:1937	Interestingly, encapsulated cells self-assembled the VH-hydrogel into spheroids, resulting in uniform distribution of both GAGs and collagen type II with increased compressive mechanical properties, rendering VH a permissive native ECM source to fabricate cartilaginous hydrogels for potential TE applications.
30572166	2	132	theme	dense	314:318	arg1	structure					320:328	its dense structure	310:328	its dense structure	310:328	However, removing DNA from cartilage (dCart) requires harsh treatments due to its dense structure, causing loss of bioactivity and limiting its application as a cartilaginous extra cellular matrix (ECM).
30572166	11	133	attach	present	2300:2306	arg2	we					2297:2298	we	2297:2298	we	2297:2298	In this study, we present an alternative route to fabricate highly permissive and bioactive ECM hydrogels from vitreous humor (VH) tissue.
30572166	11	133	attach	present	2300:2306	arg1	study					2290:2294	this study	2285:2294	this study	2285:2294	In this study, we present an alternative route to fabricate highly permissive and bioactive ECM hydrogels from vitreous humor (VH) tissue.
30572166	11	134	theme	humor	2402:2406	arg1	tissue					2413:2418	vitreous humor (VH) tissue	2393:2418	vitreous humor (VH) tissue	2393:2418	In this study, we present an alternative route to fabricate highly permissive and bioactive ECM hydrogels from vitreous humor (VH) tissue.
30572166	11	135	theme	bioactive	2364:2372	arg1	hydrogels					2378:2386	highly permissive and bioactive ECM hydrogels	2342:2386	highly permissive and bioactive ECM hydrogels	2342:2386	In this study, we present an alternative route to fabricate highly permissive and bioactive ECM hydrogels from vitreous humor (VH) tissue.
30572166	2	136	theme	bioactivity	347:357	arg1	loss					339:342	loss	339:342	loss of bioactivity	339:357	However, removing DNA from cartilage (dCart) requires harsh treatments due to its dense structure, causing loss of bioactivity and limiting its application as a cartilaginous extra cellular matrix (ECM).
30572166	10	137	theme	derived	2047:2053	arg1	biomaterials					2055:2066	SIGNIFICANCE: Fabricating bioactive and cell-instructive cartilage extracellular matrix (ECM) derived biomaterials	1953:2066	SIGNIFICANCE: Fabricating bioactive and cell-instructive cartilage extracellular matrix (ECM) derived biomaterials	1953:2066	STATEMENT OF SIGNIFICANCE: Fabricating bioactive and cell-instructive cartilage extracellular matrix (ECM) derived biomaterials and hydrogels has over recent years proven to be a challenging task, often limited by poor retention of inherent environmental cues post decellularisation due to the dense and avascular nature of native cartilage.
30260008	0	0	theme	rats	86:89	arg1	hippocampus					71:81	hippocampus	71:81	hippocampus of rats in lipopolysaccharide-induced hypocampal neuroinflammation in rats	71:156	Ouabain attenuates oxidative stress and modulates lipid composition in hippocampus of rats in lipopolysaccharide-induced hypocampal neuroinflammation in rats.
30260008	3	1	theme	protein	605:611	arg1	PCO					623:625	PCO	623:625	PCO	623:625	To reach our goal, activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX), in addition to levels of reduced glutathione (GSH), protein carbonyl (PCO) and lipid peroxidation (LPO) were evaluated.
30260008	3	1	theme	protein	605:611	arg1	carbonyl					613:620	protein carbonyl	605:620	protein carbonyl (PCO)	605:626	To reach our goal, activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX), in addition to levels of reduced glutathione (GSH), protein carbonyl (PCO) and lipid peroxidation (LPO) were evaluated.
30260008	1	2	from	effect	190:195	arg1	changes					265:271	lipopolysaccharide (LPS)-induced changes	232:271	lipopolysaccharide (LPS)-induced changes in hippocampus of rats	232:294	Our study aimed to analyze the effect of ouabain (OUA) administration on lipopolysaccharide (LPS)-induced changes in hippocampus of rats.
30260008	6	3	theme	PI	1193:1194	arg1	PI					1193:1194	PI	1193:1194	PI (60%)	1193:1200	Conversely, administration of LPS decreased GSH levels (55%), and GPx activity (30%), besides a reduction in the amount of PI (60%) and PC (45%).
30260008	6	3	theme	PI	1193:1194	arg1	%					1199:1199	60%	1197:1199	60%	1197:1199	Conversely, administration of LPS decreased GSH levels (55%), and GPx activity (30%), besides a reduction in the amount of PI (60%) and PC (45%).
30260008	6	3	theme	PI	1193:1194	arg1	amount					1183:1188	the amount	1179:1188	the amount of PI (60%) and PC (45%)	1179:1213	Conversely, administration of LPS decreased GSH levels (55%), and GPx activity (30%), besides a reduction in the amount of PI (60%) and PC (45%).
30260008	6	3	theme	PI	1193:1194	arg1	%					1212:1212	45%	1210:1212	45%	1210:1212	Conversely, administration of LPS decreased GSH levels (55%), and GPx activity (30%), besides a reduction in the amount of PI (60%) and PC (45%).
30260008	6	3	theme	PI	1193:1194	arg1	PC					1206:1207	PC	1206:1207	PC (45%)	1206:1213	Conversely, administration of LPS decreased GSH levels (55%), and GPx activity (30%), besides a reduction in the amount of PI (60%) and PC (45%).
30260008	10	4	theme	related	1638:1644	arg1	issues					1646:1651	related issues	1638:1651	related issues	1638:1651	Other studies on other cardenolides and substances related issues, as well as the development of new molecules derived from OUA, could also be useful in general oxidative and/or cellular stress, a condition favoring the appearance of neuronal pathologies.
30260008	6	5	theme	GPx	1136:1138	arg1	activity					1140:1147	GPx activity	1136:1147	GPx activity (30%)	1136:1153	Conversely, administration of LPS decreased GSH levels (55%), and GPx activity (30%), besides a reduction in the amount of PI (60%) and PC (45%).
30260008	6	5	theme	GPx	1136:1138	arg1	%					1152:1152	30%	1150:1152	30%	1150:1152	Conversely, administration of LPS decreased GSH levels (55%), and GPx activity (30%), besides a reduction in the amount of PI (60%) and PC (45%).
30260008	8	6	theme	LPS-induced	1391:1401	arg1	stress					1413:1418	LPS-induced oxidative stress	1391:1418	LPS-induced oxidative stress	1391:1418	All harmful effects recorded were attenuated by OUA, suggesting a protective effect against LPS-induced oxidative stress.
30260008	2	7	theme	intraperitoneal	353:367	arg1	injection					369:377	intraperitoneal injection	353:377	intraperitoneal injection of OUA (1.8 µg/kg), LPS (200 µg/kg), or OUA plus LPS or saline	353:440	Oxidative parameters were analyzed in Wistar rats after intraperitoneal injection of OUA (1.8 µg/kg), LPS (200 µg/kg), or OUA plus LPS or saline.
30260008	9	8	theme	oxidative	1476:1484	arg1	parameters					1486:1495	oxidative parameters	1476:1495	oxidative parameters induced by LPS	1476:1510	The relevance of our results extends beyond changes in oxidative parameters induced by LPS, because nanomolar doses of OUA may be useful in neurodegenerative models.
30260008	1	9	theme	-induced	256:263	arg1	changes					265:271	lipopolysaccharide (LPS)-induced changes	232:271	lipopolysaccharide (LPS)-induced changes in hippocampus of rats	232:294	Our study aimed to analyze the effect of ouabain (OUA) administration on lipopolysaccharide (LPS)-induced changes in hippocampus of rats.
30260008	0	10	theme	lipopolysaccharide-induced	94:119	arg1	neuroinflammation					132:148	lipopolysaccharide-induced hypocampal neuroinflammation	94:148	lipopolysaccharide-induced hypocampal neuroinflammation in rats	94:156	Ouabain attenuates oxidative stress and modulates lipid composition in hippocampus of rats in lipopolysaccharide-induced hypocampal neuroinflammation in rats.
30260008	10	11	from	studies	1593:1599	arg1	substances					1627:1636	substances	1627:1636	substances	1627:1636	Other studies on other cardenolides and substances related issues, as well as the development of new molecules derived from OUA, could also be useful in general oxidative and/or cellular stress, a condition favoring the appearance of neuronal pathologies.
30260008	10	11	from	studies	1593:1599	arg1	cardenolides					1610:1621	other cardenolides	1604:1621	other cardenolides	1604:1621	Other studies on other cardenolides and substances related issues, as well as the development of new molecules derived from OUA, could also be useful in general oxidative and/or cellular stress, a condition favoring the appearance of neuronal pathologies.
30260008	3	12	theme	catalase	504:511	arg1	activities					462:471	activities	462:471	activities	462:471	To reach our goal, activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX), in addition to levels of reduced glutathione (GSH), protein carbonyl (PCO) and lipid peroxidation (LPO) were evaluated.
30260008	0	13	from	composition	56:66	arg1	hippocampus					71:81	hippocampus	71:81	hippocampus of rats in lipopolysaccharide-induced hypocampal neuroinflammation in rats	71:156	Ouabain attenuates oxidative stress and modulates lipid composition in hippocampus of rats in lipopolysaccharide-induced hypocampal neuroinflammation in rats.
30260008	4	14	theme	nervous	751:757	arg1	system					759:764	the nervous system	747:764	the nervous system	747:764	We also analyzed the membrane lipid profile and some important lipids for the nervous system, such as phosphatidylethanolamine (PE), phosphatidylcholine (PC), phosphatidylinositol (PI), phosphatidic acid and sphingomyelin.
30260008	10	15	theme	new	1684:1686	arg1	molecules					1688:1696	new molecules	1684:1696	new molecules derived from OUA	1684:1713	Other studies on other cardenolides and substances related issues, as well as the development of new molecules derived from OUA, could also be useful in general oxidative and/or cellular stress, a condition favoring the appearance of neuronal pathologies.
30260008	10	16	from	oxidative	1748:1756	arg1	development					1669:1679	the development	1665:1679	the development of new molecules derived from OUA	1665:1713	Other studies on other cardenolides and substances related issues, as well as the development of new molecules derived from OUA, could also be useful in general oxidative and/or cellular stress, a condition favoring the appearance of neuronal pathologies.
30260008	10	16	from	oxidative	1748:1756	arg1	useful					1730:1735	useful	1730:1735	useful	1730:1735	Other studies on other cardenolides and substances related issues, as well as the development of new molecules derived from OUA, could also be useful in general oxidative and/or cellular stress, a condition favoring the appearance of neuronal pathologies.
30260008	7	17	theme	PC	1289:1290	arg1	%					1271:1271	45%	1269:1271	45%	1269:1271	By other side, OUA alone increased the amount of PI (45%), PE (85%), and PC (70%).
30260008	7	17	theme	PC	1289:1290	arg1	%					1281:1281	85%	1279:1281	85%	1279:1281	By other side, OUA alone increased the amount of PI (45%), PE (85%), and PC (70%).
30260008	7	17	theme	PC	1289:1290	arg1	%					1295:1295	70%	1293:1295	70%	1293:1295	By other side, OUA alone increased the amount of PI (45%), PE (85%), and PC (70%).
30260008	7	17	theme	PC	1289:1290	arg1	amount					1255:1260	the amount	1251:1260	the amount of PI (45%), PE (85%), and PC (70%)	1251:1296	By other side, OUA alone increased the amount of PI (45%), PE (85%), and PC (70%).
30260008	7	17	theme	PC	1289:1290	arg1	PE					1275:1276	PE	1275:1276	PE (85%)	1275:1282	By other side, OUA alone increased the amount of PI (45%), PE (85%), and PC (70%).
30260008	7	17	theme	PC	1289:1290	arg1	PC					1289:1290	PC	1289:1290	PC (70%)	1289:1296	By other side, OUA alone increased the amount of PI (45%), PE (85%), and PC (70%).
30260008	7	17	theme	PC	1289:1290	arg1	PI					1265:1266	PI	1265:1266	PI (45%)	1265:1272	By other side, OUA alone increased the amount of PI (45%), PE (85%), and PC (70%).
30260008	8	18	theme	protective	1365:1374	arg1	effect					1376:1381	a protective effect	1363:1381	a protective effect against LPS-induced oxidative stress	1363:1418	All harmful effects recorded were attenuated by OUA, suggesting a protective effect against LPS-induced oxidative stress.
30260008	8	19	theme	oxidative	1403:1411	arg1	stress					1413:1418	LPS-induced oxidative stress	1391:1418	LPS-induced oxidative stress	1391:1418	All harmful effects recorded were attenuated by OUA, suggesting a protective effect against LPS-induced oxidative stress.
30260008	3	20	theme	dismutase	487:495	arg1	activities					462:471	activities	462:471	activities	462:471	To reach our goal, activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX), in addition to levels of reduced glutathione (GSH), protein carbonyl (PCO) and lipid peroxidation (LPO) were evaluated.
30260008	9	21	from	models	1579:1584	arg1	useful					1551:1556	useful	1551:1556	useful	1551:1556	The relevance of our results extends beyond changes in oxidative parameters induced by LPS, because nanomolar doses of OUA may be useful in neurodegenerative models.
30260008	0	22	theme	hypocampal	121:130	arg1	neuroinflammation					132:148	lipopolysaccharide-induced hypocampal neuroinflammation	94:148	lipopolysaccharide-induced hypocampal neuroinflammation in rats	94:156	Ouabain attenuates oxidative stress and modulates lipid composition in hippocampus of rats in lipopolysaccharide-induced hypocampal neuroinflammation in rats.
30260008	3	23	theme	carbonyl	613:620	arg1	levels					568:573	levels	568:573	levels of reduced glutathione (GSH), protein carbonyl (PCO) and lipid peroxidation (LPO)	568:655	To reach our goal, activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX), in addition to levels of reduced glutathione (GSH), protein carbonyl (PCO) and lipid peroxidation (LPO) were evaluated.
30260008	10	24	theme	neuronal	1821:1828	arg1	pathologies					1830:1840	neuronal pathologies	1821:1840	neuronal pathologies	1821:1840	Other studies on other cardenolides and substances related issues, as well as the development of new molecules derived from OUA, could also be useful in general oxidative and/or cellular stress, a condition favoring the appearance of neuronal pathologies.
30260008	10	25	theme	pathologies	1830:1840	arg1	appearance					1807:1816	the appearance	1803:1816	the appearance of neuronal pathologies	1803:1840	Other studies on other cardenolides and substances related issues, as well as the development of new molecules derived from OUA, could also be useful in general oxidative and/or cellular stress, a condition favoring the appearance of neuronal pathologies.
30260008	10	26	theme	molecules	1688:1696	arg1	development					1669:1679	the development	1665:1679	the development of new molecules derived from OUA	1665:1713	Other studies on other cardenolides and substances related issues, as well as the development of new molecules derived from OUA, could also be useful in general oxidative and/or cellular stress, a condition favoring the appearance of neuronal pathologies.
30260008	10	26	theme	molecules	1688:1696	arg1	studies					1593:1599	Other studies	1587:1599	Other studies on other cardenolides and substances related issues	1587:1651	Other studies on other cardenolides and substances related issues, as well as the development of new molecules derived from OUA, could also be useful in general oxidative and/or cellular stress, a condition favoring the appearance of neuronal pathologies.
30260008	10	26	theme	molecules	1688:1696	arg1	useful					1730:1735	useful	1730:1735	useful	1730:1735	Other studies on other cardenolides and substances related issues, as well as the development of new molecules derived from OUA, could also be useful in general oxidative and/or cellular stress, a condition favoring the appearance of neuronal pathologies.
30260008	5	27	theme	PCO	1014:1016	arg1	levels					1038:1043	PCO (about three times) levels	1014:1043	PCO (about three times) levels	1014:1043	The group that received only LPS showed increased oxidative stress, as evidenced by an increase in LPO (about twice), PCO (about three times) levels, and CAT activity (80%).
30260008	7	28	theme	PE	1275:1276	arg1	%					1271:1271	45%	1269:1271	45%	1269:1271	By other side, OUA alone increased the amount of PI (45%), PE (85%), and PC (70%).
30260008	7	28	theme	PE	1275:1276	arg1	%					1281:1281	85%	1279:1281	85%	1279:1281	By other side, OUA alone increased the amount of PI (45%), PE (85%), and PC (70%).
30260008	7	28	theme	PE	1275:1276	arg1	%					1295:1295	70%	1293:1295	70%	1293:1295	By other side, OUA alone increased the amount of PI (45%), PE (85%), and PC (70%).
30260008	7	28	theme	PE	1275:1276	arg1	amount					1255:1260	the amount	1251:1260	the amount of PI (45%), PE (85%), and PC (70%)	1251:1296	By other side, OUA alone increased the amount of PI (45%), PE (85%), and PC (70%).
30260008	7	28	theme	PE	1275:1276	arg1	PE					1275:1276	PE	1275:1276	PE (85%)	1275:1282	By other side, OUA alone increased the amount of PI (45%), PE (85%), and PC (70%).
30260008	7	28	theme	PE	1275:1276	arg1	PC					1289:1290	PC	1289:1290	PC (70%)	1289:1296	By other side, OUA alone increased the amount of PI (45%), PE (85%), and PC (70%).
30260008	7	28	theme	PE	1275:1276	arg1	PI					1265:1266	PI	1265:1266	PI (45%)	1265:1272	By other side, OUA alone increased the amount of PI (45%), PE (85%), and PC (70%).
30260008	10	29	attach	derived	1698:1704	arg1	OUA					1711:1713	OUA	1711:1713	OUA	1711:1713	Other studies on other cardenolides and substances related issues, as well as the development of new molecules derived from OUA, could also be useful in general oxidative and/or cellular stress, a condition favoring the appearance of neuronal pathologies.
30260008	10	29	attach	derived	1698:1704	arg2	molecules					1688:1696	new molecules	1684:1696	new molecules derived from OUA	1684:1713	Other studies on other cardenolides and substances related issues, as well as the development of new molecules derived from OUA, could also be useful in general oxidative and/or cellular stress, a condition favoring the appearance of neuronal pathologies.
30260008	5	30	dep	LPO	995:997	arg1	twice					1006:1010	twice	1006:1010	twice	1006:1010	The group that received only LPS showed increased oxidative stress, as evidenced by an increase in LPO (about twice), PCO (about three times) levels, and CAT activity (80%).
30260008	2	31	theme	OUA	419:421	arg1	injection					369:377	intraperitoneal injection	353:377	intraperitoneal injection of OUA (1.8 µg/kg), LPS (200 µg/kg), or OUA plus LPS or saline	353:440	Oxidative parameters were analyzed in Wistar rats after intraperitoneal injection of OUA (1.8 µg/kg), LPS (200 µg/kg), or OUA plus LPS or saline.
30260008	3	32	theme	peroxidation	638:649	arg1	levels					568:573	levels	568:573	levels of reduced glutathione (GSH), protein carbonyl (PCO) and lipid peroxidation (LPO)	568:655	To reach our goal, activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX), in addition to levels of reduced glutathione (GSH), protein carbonyl (PCO) and lipid peroxidation (LPO) were evaluated.
30260008	3	33	theme	reduced	578:584	arg1	GSH					599:601	GSH	599:601	GSH	599:601	To reach our goal, activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX), in addition to levels of reduced glutathione (GSH), protein carbonyl (PCO) and lipid peroxidation (LPO) were evaluated.
30260008	3	33	theme	reduced	578:584	arg1	glutathione					586:596	glutathione	586:596	reduced glutathione (GSH)	578:602	To reach our goal, activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX), in addition to levels of reduced glutathione (GSH), protein carbonyl (PCO) and lipid peroxidation (LPO) were evaluated.
30260008	3	34	theme	glutathione	586:596	arg1	levels					568:573	levels	568:573	levels of reduced glutathione (GSH), protein carbonyl (PCO) and lipid peroxidation (LPO)	568:655	To reach our goal, activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX), in addition to levels of reduced glutathione (GSH), protein carbonyl (PCO) and lipid peroxidation (LPO) were evaluated.
30260008	0	35	theme	lipid	50:54	arg1	composition					56:66	lipid composition	50:66	lipid composition in hippocampus of rats in lipopolysaccharide-induced hypocampal neuroinflammation in rats	50:156	Ouabain attenuates oxidative stress and modulates lipid composition in hippocampus of rats in lipopolysaccharide-induced hypocampal neuroinflammation in rats.
30260008	1	36	theme	ouabain	200:206	arg1	administration					214:227	ouabain (OUA) administration	200:227	ouabain (OUA) administration	200:227	Our study aimed to analyze the effect of ouabain (OUA) administration on lipopolysaccharide (LPS)-induced changes in hippocampus of rats.
30260008	1	37	from	changes	265:271	arg1	hippocampus					276:286	hippocampus	276:286	hippocampus of rats	276:294	Our study aimed to analyze the effect of ouabain (OUA) administration on lipopolysaccharide (LPS)-induced changes in hippocampus of rats.
30260008	4	38	theme	membrane	694:701	arg1	profile					709:715	the membrane lipid profile	690:715	the membrane lipid profile	690:715	We also analyzed the membrane lipid profile and some important lipids for the nervous system, such as phosphatidylethanolamine (PE), phosphatidylcholine (PC), phosphatidylinositol (PI), phosphatidic acid and sphingomyelin.
30260008	0	39	theme	oxidative	19:27	arg1	stress					29:34	oxidative stress	19:34	oxidative stress	19:34	Ouabain attenuates oxidative stress and modulates lipid composition in hippocampus of rats in lipopolysaccharide-induced hypocampal neuroinflammation in rats.
30260008	1	40	theme	rats	291:294	arg1	hippocampus					276:286	hippocampus	276:286	hippocampus of rats	276:294	Our study aimed to analyze the effect of ouabain (OUA) administration on lipopolysaccharide (LPS)-induced changes in hippocampus of rats.
30260008	5	41	from	activity	1054:1061	arg1	LPO					995:997	LPO	995:997	LPO (about twice)	995:1011	The group that received only LPS showed increased oxidative stress, as evidenced by an increase in LPO (about twice), PCO (about three times) levels, and CAT activity (80%).
30260008	6	42	from	reduction	1166:1174	arg1	PI					1193:1194	PI	1193:1194	PI (60%)	1193:1200	Conversely, administration of LPS decreased GSH levels (55%), and GPx activity (30%), besides a reduction in the amount of PI (60%) and PC (45%).
30260008	6	42	from	reduction	1166:1174	arg1	%					1199:1199	60%	1197:1199	60%	1197:1199	Conversely, administration of LPS decreased GSH levels (55%), and GPx activity (30%), besides a reduction in the amount of PI (60%) and PC (45%).
30260008	6	42	from	reduction	1166:1174	arg1	amount					1183:1188	the amount	1179:1188	the amount of PI (60%) and PC (45%)	1179:1213	Conversely, administration of LPS decreased GSH levels (55%), and GPx activity (30%), besides a reduction in the amount of PI (60%) and PC (45%).
30260008	6	42	from	reduction	1166:1174	arg1	%					1212:1212	45%	1210:1212	45%	1210:1212	Conversely, administration of LPS decreased GSH levels (55%), and GPx activity (30%), besides a reduction in the amount of PI (60%) and PC (45%).
30260008	6	42	from	reduction	1166:1174	arg1	PC					1206:1207	PC	1206:1207	PC (45%)	1206:1213	Conversely, administration of LPS decreased GSH levels (55%), and GPx activity (30%), besides a reduction in the amount of PI (60%) and PC (45%).
30260008	2	43	theme	Oxidative	297:305	arg1	parameters					307:316	Oxidative parameters	297:316	Oxidative parameters	297:316	Oxidative parameters were analyzed in Wistar rats after intraperitoneal injection of OUA (1.8 µg/kg), LPS (200 µg/kg), or OUA plus LPS or saline.
30260008	1	44	theme	OUA	209:211	arg1	administration					214:227	ouabain (OUA) administration	200:227	ouabain (OUA) administration	200:227	Our study aimed to analyze the effect of ouabain (OUA) administration on lipopolysaccharide (LPS)-induced changes in hippocampus of rats.
30260008	5	45	from	increase	983:990	arg1	LPO					995:997	LPO	995:997	LPO (about twice)	995:1011	The group that received only LPS showed increased oxidative stress, as evidenced by an increase in LPO (about twice), PCO (about three times) levels, and CAT activity (80%).
30260008	3	46	theme	lipid	632:636	arg1	peroxidation					638:649	lipid peroxidation	632:649	lipid peroxidation (LPO)	632:655	To reach our goal, activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX), in addition to levels of reduced glutathione (GSH), protein carbonyl (PCO) and lipid peroxidation (LPO) were evaluated.
30260008	3	46	theme	lipid	632:636	arg1	LPO					652:654	LPO	652:654	LPO	652:654	To reach our goal, activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX), in addition to levels of reduced glutathione (GSH), protein carbonyl (PCO) and lipid peroxidation (LPO) were evaluated.
30260008	7	47	theme	other	1219:1223	arg1	side					1225:1228	other side	1219:1228	other side	1219:1228	By other side, OUA alone increased the amount of PI (45%), PE (85%), and PC (70%).
30260008	3	48	theme	glutathione	523:533	arg1	GPX					547:549	GPX	547:549	GPX	547:549	To reach our goal, activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX), in addition to levels of reduced glutathione (GSH), protein carbonyl (PCO) and lipid peroxidation (LPO) were evaluated.
30260008	3	48	theme	glutathione	523:533	arg1	peroxidase					535:544	glutathione peroxidase	523:544	glutathione peroxidase (GPX)	523:550	To reach our goal, activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX), in addition to levels of reduced glutathione (GSH), protein carbonyl (PCO) and lipid peroxidation (LPO) were evaluated.
30260008	6	49	theme	PC	1206:1207	arg1	PI					1193:1194	PI	1193:1194	PI (60%)	1193:1200	Conversely, administration of LPS decreased GSH levels (55%), and GPx activity (30%), besides a reduction in the amount of PI (60%) and PC (45%).
30260008	6	49	theme	PC	1206:1207	arg1	%					1199:1199	60%	1197:1199	60%	1197:1199	Conversely, administration of LPS decreased GSH levels (55%), and GPx activity (30%), besides a reduction in the amount of PI (60%) and PC (45%).
30260008	6	49	theme	PC	1206:1207	arg1	amount					1183:1188	the amount	1179:1188	the amount of PI (60%) and PC (45%)	1179:1213	Conversely, administration of LPS decreased GSH levels (55%), and GPx activity (30%), besides a reduction in the amount of PI (60%) and PC (45%).
30260008	6	49	theme	PC	1206:1207	arg1	%					1212:1212	45%	1210:1212	45%	1210:1212	Conversely, administration of LPS decreased GSH levels (55%), and GPx activity (30%), besides a reduction in the amount of PI (60%) and PC (45%).
30260008	6	49	theme	PC	1206:1207	arg1	PC					1206:1207	PC	1206:1207	PC (45%)	1206:1213	Conversely, administration of LPS decreased GSH levels (55%), and GPx activity (30%), besides a reduction in the amount of PI (60%) and PC (45%).
30260008	10	50	from	stress	1774:1779	arg1	development					1669:1679	the development	1665:1679	the development of new molecules derived from OUA	1665:1713	Other studies on other cardenolides and substances related issues, as well as the development of new molecules derived from OUA, could also be useful in general oxidative and/or cellular stress, a condition favoring the appearance of neuronal pathologies.
30260008	10	50	from	stress	1774:1779	arg1	useful					1730:1735	useful	1730:1735	useful	1730:1735	Other studies on other cardenolides and substances related issues, as well as the development of new molecules derived from OUA, could also be useful in general oxidative and/or cellular stress, a condition favoring the appearance of neuronal pathologies.
30260008	10	51	from	useful	1730:1735	arg1	condition					1784:1792	a condition	1782:1792	a condition favoring the appearance of neuronal pathologies	1782:1840	Other studies on other cardenolides and substances related issues, as well as the development of new molecules derived from OUA, could also be useful in general oxidative and/or cellular stress, a condition favoring the appearance of neuronal pathologies.
30260008	10	51	from	useful	1730:1735	arg1	oxidative					1748:1756	oxidative	1748:1756	oxidative	1748:1756	Other studies on other cardenolides and substances related issues, as well as the development of new molecules derived from OUA, could also be useful in general oxidative and/or cellular stress, a condition favoring the appearance of neuronal pathologies.
30260008	10	51	from	useful	1730:1735	arg1	stress					1774:1779	general oxidative and/or cellular stress	1740:1779	stress	1774:1779	Other studies on other cardenolides and substances related issues, as well as the development of new molecules derived from OUA, could also be useful in general oxidative and/or cellular stress, a condition favoring the appearance of neuronal pathologies.
30260008	4	52	theme	phosphatidic	859:870	arg1	acid					872:875	phosphatidic acid	859:875	phosphatidic acid	859:875	We also analyzed the membrane lipid profile and some important lipids for the nervous system, such as phosphatidylethanolamine (PE), phosphatidylcholine (PC), phosphatidylinositol (PI), phosphatidic acid and sphingomyelin.
30260008	6	53	theme	GSH	1114:1116	arg1	levels					1118:1123	GSH levels	1114:1123	GSH levels (55%)	1114:1129	Conversely, administration of LPS decreased GSH levels (55%), and GPx activity (30%), besides a reduction in the amount of PI (60%) and PC (45%).
30260008	6	53	theme	GSH	1114:1116	arg1	%					1128:1128	55%	1126:1128	55%	1126:1128	Conversely, administration of LPS decreased GSH levels (55%), and GPx activity (30%), besides a reduction in the amount of PI (60%) and PC (45%).
30260008	0	54	from	hippocampus	71:81	arg1	neuroinflammation					132:148	lipopolysaccharide-induced hypocampal neuroinflammation	94:148	lipopolysaccharide-induced hypocampal neuroinflammation in rats	94:156	Ouabain attenuates oxidative stress and modulates lipid composition in hippocampus of rats in lipopolysaccharide-induced hypocampal neuroinflammation in rats.
30260008	3	55	theme	peroxidase	535:544	arg1	activities					462:471	activities	462:471	activities	462:471	To reach our goal, activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX), in addition to levels of reduced glutathione (GSH), protein carbonyl (PCO) and lipid peroxidation (LPO) were evaluated.
30260008	9	56	theme	OUA	1540:1542	arg1	doses					1531:1535	nanomolar doses	1521:1535	nanomolar doses of OUA	1521:1542	The relevance of our results extends beyond changes in oxidative parameters induced by LPS, because nanomolar doses of OUA may be useful in neurodegenerative models.
30260008	2	57	theme	LPS	399:401	arg1	injection					369:377	intraperitoneal injection	353:377	intraperitoneal injection of OUA (1.8 µg/kg), LPS (200 µg/kg), or OUA plus LPS or saline	353:440	Oxidative parameters were analyzed in Wistar rats after intraperitoneal injection of OUA (1.8 µg/kg), LPS (200 µg/kg), or OUA plus LPS or saline.
30260008	9	58	from	changes	1465:1471	arg1	parameters					1486:1495	oxidative parameters	1476:1495	oxidative parameters induced by LPS	1476:1510	The relevance of our results extends beyond changes in oxidative parameters induced by LPS, because nanomolar doses of OUA may be useful in neurodegenerative models.
30260008	5	59	theme	oxidative	946:954	arg1	stress					956:961	oxidative stress	946:961	oxidative stress	946:961	The group that received only LPS showed increased oxidative stress, as evidenced by an increase in LPO (about twice), PCO (about three times) levels, and CAT activity (80%).
30260008	4	60	theme	important	726:734	arg1	phosphatidylethanolamine					775:798	phosphatidylethanolamine	775:798	phosphatidylethanolamine (PE)	775:803	We also analyzed the membrane lipid profile and some important lipids for the nervous system, such as phosphatidylethanolamine (PE), phosphatidylcholine (PC), phosphatidylinositol (PI), phosphatidic acid and sphingomyelin.
30260008	4	60	theme	important	726:734	arg1	lipids					736:741	some important lipids	721:741	some important lipids	721:741	We also analyzed the membrane lipid profile and some important lipids for the nervous system, such as phosphatidylethanolamine (PE), phosphatidylcholine (PC), phosphatidylinositol (PI), phosphatidic acid and sphingomyelin.
30260008	9	61	theme	nanomolar	1521:1529	arg1	doses					1531:1535	nanomolar doses	1521:1535	nanomolar doses of OUA	1521:1542	The relevance of our results extends beyond changes in oxidative parameters induced by LPS, because nanomolar doses of OUA may be useful in neurodegenerative models.
30260008	2	62	theme	LPS	428:430	arg1	injection					369:377	intraperitoneal injection	353:377	intraperitoneal injection of OUA (1.8 µg/kg), LPS (200 µg/kg), or OUA plus LPS or saline	353:440	Oxidative parameters were analyzed in Wistar rats after intraperitoneal injection of OUA (1.8 µg/kg), LPS (200 µg/kg), or OUA plus LPS or saline.
30260008	7	63	theme	PI	1265:1266	arg1	%					1271:1271	45%	1269:1271	45%	1269:1271	By other side, OUA alone increased the amount of PI (45%), PE (85%), and PC (70%).
30260008	7	63	theme	PI	1265:1266	arg1	%					1281:1281	85%	1279:1281	85%	1279:1281	By other side, OUA alone increased the amount of PI (45%), PE (85%), and PC (70%).
30260008	7	63	theme	PI	1265:1266	arg1	%					1295:1295	70%	1293:1295	70%	1293:1295	By other side, OUA alone increased the amount of PI (45%), PE (85%), and PC (70%).
30260008	7	63	theme	PI	1265:1266	arg1	amount					1255:1260	the amount	1251:1260	the amount of PI (45%), PE (85%), and PC (70%)	1251:1296	By other side, OUA alone increased the amount of PI (45%), PE (85%), and PC (70%).
30260008	7	63	theme	PI	1265:1266	arg1	PE					1275:1276	PE	1275:1276	PE (85%)	1275:1282	By other side, OUA alone increased the amount of PI (45%), PE (85%), and PC (70%).
30260008	7	63	theme	PI	1265:1266	arg1	PC					1289:1290	PC	1289:1290	PC (70%)	1289:1296	By other side, OUA alone increased the amount of PI (45%), PE (85%), and PC (70%).
30260008	7	63	theme	PI	1265:1266	arg1	PI					1265:1266	PI	1265:1266	PI (45%)	1265:1272	By other side, OUA alone increased the amount of PI (45%), PE (85%), and PC (70%).
30260008	6	64	theme	LPS	1100:1102	arg1	administration					1082:1095	administration	1082:1095	administration of LPS	1082:1102	Conversely, administration of LPS decreased GSH levels (55%), and GPx activity (30%), besides a reduction in the amount of PI (60%) and PC (45%).
30260008	0	65	from	neuroinflammation	132:148	arg1	rats					153:156	rats	153:156	rats	153:156	Ouabain attenuates oxidative stress and modulates lipid composition in hippocampus of rats in lipopolysaccharide-induced hypocampal neuroinflammation in rats.
30260008	4	66	theme	lipid	703:707	arg1	profile					709:715	the membrane lipid profile	690:715	the membrane lipid profile	690:715	We also analyzed the membrane lipid profile and some important lipids for the nervous system, such as phosphatidylethanolamine (PE), phosphatidylcholine (PC), phosphatidylinositol (PI), phosphatidic acid and sphingomyelin.
30260008	9	67	from	useful	1551:1556	arg1	models					1579:1584	neurodegenerative models	1561:1584	neurodegenerative models	1561:1584	The relevance of our results extends beyond changes in oxidative parameters induced by LPS, because nanomolar doses of OUA may be useful in neurodegenerative models.
30260008	5	68	from	levels	1038:1043	arg1	LPO					995:997	LPO	995:997	LPO (about twice)	995:1011	The group that received only LPS showed increased oxidative stress, as evidenced by an increase in LPO (about twice), PCO (about three times) levels, and CAT activity (80%).
30260008	10	69	dep	cardenolides	1610:1621	arg1	issues					1646:1651	related issues	1638:1651	related issues	1638:1651	Other studies on other cardenolides and substances related issues, as well as the development of new molecules derived from OUA, could also be useful in general oxidative and/or cellular stress, a condition favoring the appearance of neuronal pathologies.
30260008	2	70	theme	Wistar	335:340	arg1	rats					342:345	Wistar rats	335:345	Wistar rats	335:345	Oxidative parameters were analyzed in Wistar rats after intraperitoneal injection of OUA (1.8 µg/kg), LPS (200 µg/kg), or OUA plus LPS or saline.
30260008	9	71	theme	results	1442:1448	arg1	relevance					1425:1433	The relevance	1421:1433	The relevance of our results	1421:1448	The relevance of our results extends beyond changes in oxidative parameters induced by LPS, because nanomolar doses of OUA may be useful in neurodegenerative models.
30260008	10	72	theme	other	1604:1608	arg1	cardenolides					1610:1621	other cardenolides	1604:1621	other cardenolides	1604:1621	Other studies on other cardenolides and substances related issues, as well as the development of new molecules derived from OUA, could also be useful in general oxidative and/or cellular stress, a condition favoring the appearance of neuronal pathologies.
30260008	10	73	theme	cellular	1765:1772	arg1	stress					1774:1779	general oxidative and/or cellular stress	1740:1779	stress	1774:1779	Other studies on other cardenolides and substances related issues, as well as the development of new molecules derived from OUA, could also be useful in general oxidative and/or cellular stress, a condition favoring the appearance of neuronal pathologies.
30260008	10	74	from	development	1669:1679	arg1	substances					1627:1636	substances	1627:1636	substances	1627:1636	Other studies on other cardenolides and substances related issues, as well as the development of new molecules derived from OUA, could also be useful in general oxidative and/or cellular stress, a condition favoring the appearance of neuronal pathologies.
30260008	10	74	from	development	1669:1679	arg1	cardenolides					1610:1621	other cardenolides	1604:1621	other cardenolides	1604:1621	Other studies on other cardenolides and substances related issues, as well as the development of new molecules derived from OUA, could also be useful in general oxidative and/or cellular stress, a condition favoring the appearance of neuronal pathologies.
30260008	8	75	theme	harmful	1303:1309	arg1	effects					1311:1317	All harmful effects	1299:1317	All harmful effects recorded	1299:1326	All harmful effects recorded were attenuated by OUA, suggesting a protective effect against LPS-induced oxidative stress.
30260008	4	76	dep	phosphatidylethanolamine	775:798	arg1	acid					872:875	phosphatidic acid	859:875	phosphatidic acid	859:875	We also analyzed the membrane lipid profile and some important lipids for the nervous system, such as phosphatidylethanolamine (PE), phosphatidylcholine (PC), phosphatidylinositol (PI), phosphatidic acid and sphingomyelin.
30260008	4	76	dep	phosphatidylethanolamine	775:798	arg1	phosphatidylinositol					832:851	phosphatidylinositol	832:851	phosphatidylinositol (PI)	832:856	We also analyzed the membrane lipid profile and some important lipids for the nervous system, such as phosphatidylethanolamine (PE), phosphatidylcholine (PC), phosphatidylinositol (PI), phosphatidic acid and sphingomyelin.
30260008	4	76	dep	phosphatidylethanolamine	775:798	arg1	PI					854:855	PI	854:855	PI	854:855	We also analyzed the membrane lipid profile and some important lipids for the nervous system, such as phosphatidylethanolamine (PE), phosphatidylcholine (PC), phosphatidylinositol (PI), phosphatidic acid and sphingomyelin.
30260008	4	76	dep	phosphatidylethanolamine	775:798	arg1	sphingomyelin					881:893	sphingomyelin	881:893	sphingomyelin	881:893	We also analyzed the membrane lipid profile and some important lipids for the nervous system, such as phosphatidylethanolamine (PE), phosphatidylcholine (PC), phosphatidylinositol (PI), phosphatidic acid and sphingomyelin.
30260008	4	76	dep	phosphatidylethanolamine	775:798	arg1	phosphatidylcholine					806:824	phosphatidylcholine	806:824	phosphatidylcholine (PC)	806:829	We also analyzed the membrane lipid profile and some important lipids for the nervous system, such as phosphatidylethanolamine (PE), phosphatidylcholine (PC), phosphatidylinositol (PI), phosphatidic acid and sphingomyelin.
30260008	4	76	dep	phosphatidylethanolamine	775:798	arg1	PC					827:828	PC	827:828	PC	827:828	We also analyzed the membrane lipid profile and some important lipids for the nervous system, such as phosphatidylethanolamine (PE), phosphatidylcholine (PC), phosphatidylinositol (PI), phosphatidic acid and sphingomyelin.
30260008	1	77	theme	administration	214:227	arg1	effect					190:195	the effect	186:195	the effect of ouabain (OUA) administration on lipopolysaccharide (LPS)-induced changes in hippocampus of rats	186:294	Our study aimed to analyze the effect of ouabain (OUA) administration on lipopolysaccharide (LPS)-induced changes in hippocampus of rats.
30260008	9	78	theme	neurodegenerative	1561:1577	arg1	models					1579:1584	neurodegenerative models	1561:1584	neurodegenerative models	1561:1584	The relevance of our results extends beyond changes in oxidative parameters induced by LPS, because nanomolar doses of OUA may be useful in neurodegenerative models.
30260008	2	79	theme	OUA	382:384	arg1	injection					369:377	intraperitoneal injection	353:377	intraperitoneal injection of OUA (1.8 µg/kg), LPS (200 µg/kg), or OUA plus LPS or saline	353:440	Oxidative parameters were analyzed in Wistar rats after intraperitoneal injection of OUA (1.8 µg/kg), LPS (200 µg/kg), or OUA plus LPS or saline.
30260008	10	80	theme	Other	1587:1591	arg1	studies					1593:1599	Other studies	1587:1599	Other studies on other cardenolides and substances related issues	1587:1651	Other studies on other cardenolides and substances related issues, as well as the development of new molecules derived from OUA, could also be useful in general oxidative and/or cellular stress, a condition favoring the appearance of neuronal pathologies.
30260008	5	81	dep	showed	929:934	arg1	increased					936:944	increased	936:944	increased	936:944	The group that received only LPS showed increased oxidative stress, as evidenced by an increase in LPO (about twice), PCO (about three times) levels, and CAT activity (80%).
30260008	3	82	theme	superoxide	476:485	arg1	SOD					498:500	SOD	498:500	SOD	498:500	To reach our goal, activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX), in addition to levels of reduced glutathione (GSH), protein carbonyl (PCO) and lipid peroxidation (LPO) were evaluated.
30260008	3	82	theme	superoxide	476:485	arg1	dismutase					487:495	superoxide dismutase	476:495	superoxide dismutase (SOD)	476:501	To reach our goal, activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX), in addition to levels of reduced glutathione (GSH), protein carbonyl (PCO) and lipid peroxidation (LPO) were evaluated.
30260008	5	83	theme	CAT	1050:1052	arg1	activity					1054:1061	CAT activity	1050:1061	CAT activity (80%)	1050:1067	The group that received only LPS showed increased oxidative stress, as evidenced by an increase in LPO (about twice), PCO (about three times) levels, and CAT activity (80%).
30260008	5	83	theme	CAT	1050:1052	arg1	%					1066:1066	80%	1064:1066	80%	1064:1066	The group that received only LPS showed increased oxidative stress, as evidenced by an increase in LPO (about twice), PCO (about three times) levels, and CAT activity (80%).
31022860	12	0	theme	divaricatum	1383:1393	arg1	value					1371:1375	the value	1367:1375	the value of C. divaricatum	1367:1393	The newly identified constituents of the plant seem to be partly responsible for its pharmacological activity and elevate the value of C. divaricatum as a potential functional food.
31022860	12	0	theme	divaricatum	1383:1393	arg1	food					1421:1424	a potential functional food	1398:1424	a potential functional food	1398:1424	The newly identified constituents of the plant seem to be partly responsible for its pharmacological activity and elevate the value of C. divaricatum as a potential functional food.
31022860	9	1	theme	cultivated	804:813	arg1	shoots					815:820	cultivated shoots	804:820	cultivated shoots	804:820	Fractionation of lipophilic extract from cultivated shoots led to the isolation of 12-oxo-phytodienoic acid (12-OPDA), which is a newly identified constituent of the plant.
31022860	7	2	theme	plant	589:593	arg1	constituents					569:580	formerly unknown constituents	552:580	formerly unknown constituents of the plant	552:593	The composition of hydroalcoholic extract from aerial parts of C. divaricatum was analyzed by HPLC-DAD-MSn, revealing the presence of numerous caffeic acid derivatives that were formerly unknown constituents of the plant.
31022860	2	3	theme	Plant	102:106	arg1	Parts					89:93	Aerial Parts	82:93	Aerial Parts of the Plant	82:106	Revisited: Newly Identified Constituents from Aerial Parts of the Plant and Their Possible Contribution to the Biological Activity of the Plant.
31022860	2	4	theme	Biological	147:156	arg1	Activity					158:165	the Biological Activity	143:165	the Biological Activity of the Plant	143:178	Revisited: Newly Identified Constituents from Aerial Parts of the Plant and Their Possible Contribution to the Biological Activity of the Plant.
31022860	8	5	theme	occurring	672:680	arg1	butyryl					682:688	rarely occurring butyryl and methylbutyryl tricaffeoylhexaric acids	665:731	butyryl	682:688	In all, 17 compounds, including commonly found chlorogenic acids and rarely occurring butyryl and methylbutyryl tricaffeoylhexaric acids, were tentatively identified.
31022860	12	6	theme	identified	1255:1264	arg1	constituents					1266:1277	The newly identified constituents	1245:1277	The newly identified constituents of the plant	1245:1290	The newly identified constituents of the plant seem to be partly responsible for its pharmacological activity and elevate the value of C. divaricatum as a potential functional food.
31022860	12	6	theme	identified	1255:1264	arg1	responsible					1310:1320	responsible	1310:1320	responsible	1310:1320	The newly identified constituents of the plant seem to be partly responsible for its pharmacological activity and elevate the value of C. divaricatum as a potential functional food.
31022860	11	7	theme	oxygen	1120:1125	arg1	ROS					1136:1138	ROS	1136:1138	ROS	1136:1138	Reactive oxygen species (ROS) production induced by f-MLP was also significantly diminished in the neutrophils pretreated by 12-OPDA.
31022860	11	7	theme	oxygen	1120:1125	arg1	species					1127:1133	Reactive oxygen species	1111:1133	Reactive oxygen species (ROS) production induced by f-MLP	1111:1167	Reactive oxygen species (ROS) production induced by f-MLP was also significantly diminished in the neutrophils pretreated by 12-OPDA.
31022860	9	8	theme	plant	929:933	arg1	constituent					910:920	a newly identified constituent	891:920	a newly identified constituent of the plant	891:933	Fractionation of lipophilic extract from cultivated shoots led to the isolation of 12-oxo-phytodienoic acid (12-OPDA), which is a newly identified constituent of the plant.
31022860	9	8	theme	plant	929:933	arg1	acid					866:869	12-oxo-phytodienoic acid	846:869	12-oxo-phytodienoic acid (12-OPDA)	846:879	Fractionation of lipophilic extract from cultivated shoots led to the isolation of 12-oxo-phytodienoic acid (12-OPDA), which is a newly identified constituent of the plant.
31022860	11	9	theme	species	1127:1133	arg1	production					1141:1150	Reactive oxygen species (ROS) production	1111:1150	Reactive oxygen species (ROS) production induced by f-MLP	1111:1167	Reactive oxygen species (ROS) production induced by f-MLP was also significantly diminished in the neutrophils pretreated by 12-OPDA.
31022860	10	10	theme	human	1092:1096	arg1	neutrophils					1098:1108	lipopolysaccharide (LPS)-stimulated human neutrophils	1056:1108	lipopolysaccharide (LPS)-stimulated human neutrophils	1056:1108	The compound, at concentrations of 0.5, 1.0, and 2.5 μM, significantly reduced IL-8, IL-1β, TNFα, and CCL2 excretion by lipopolysaccharide (LPS)-stimulated human neutrophils.
31022860	8	11	theme	chlorogenic	643:653	arg1	acids					655:659	commonly found chlorogenic acids	628:659	commonly found chlorogenic acids	628:659	In all, 17 compounds, including commonly found chlorogenic acids and rarely occurring butyryl and methylbutyryl tricaffeoylhexaric acids, were tentatively identified.
31022860	7	12	from	composition	378:388	arg1	parts					428:432	aerial parts	421:432	aerial parts of C. divaricatum	421:450	The composition of hydroalcoholic extract from aerial parts of C. divaricatum was analyzed by HPLC-DAD-MSn, revealing the presence of numerous caffeic acid derivatives that were formerly unknown constituents of the plant.
31022860	5	13	theme	use	239:241	arg1	plant					274:278	a food plant	267:278	a food plant	267:278	has a long history of use as both a medicinal and a food plant.
31022860	5	13	theme	use	239:241	arg1	history					228:234	a long history	221:234	a long history of use	221:241	has a long history of use as both a medicinal and a food plant.
31022860	5	13	theme	use	239:241	arg1	medicinal					253:261	a medicinal	251:261	a medicinal	251:261	has a long history of use as both a medicinal and a food plant.
31022860	7	14	theme	unknown	561:567	arg1	constituents					569:580	formerly unknown constituents	552:580	formerly unknown constituents of the plant	552:593	The composition of hydroalcoholic extract from aerial parts of C. divaricatum was analyzed by HPLC-DAD-MSn, revealing the presence of numerous caffeic acid derivatives that were formerly unknown constituents of the plant.
31022860	10	15	theme	-stimulated	1080:1090	arg1	neutrophils					1098:1108	lipopolysaccharide (LPS)-stimulated human neutrophils	1056:1108	lipopolysaccharide (LPS)-stimulated human neutrophils	1056:1108	The compound, at concentrations of 0.5, 1.0, and 2.5 μM, significantly reduced IL-8, IL-1β, TNFα, and CCL2 excretion by lipopolysaccharide (LPS)-stimulated human neutrophils.
31022860	2	16	theme	Possible	118:125	arg1	Contribution					127:138	Their Possible Contribution	112:138	Their Possible Contribution to the Biological Activity of the Plant	112:178	Revisited: Newly Identified Constituents from Aerial Parts of the Plant and Their Possible Contribution to the Biological Activity of the Plant.
31022860	10	17	theme	CCL2	1038:1041	arg1	excretion					1043:1051	CCL2 excretion	1038:1051	CCL2 excretion	1038:1051	The compound, at concentrations of 0.5, 1.0, and 2.5 μM, significantly reduced IL-8, IL-1β, TNFα, and CCL2 excretion by lipopolysaccharide (LPS)-stimulated human neutrophils.
31022860	7	18	theme	divaricatum	440:450	arg1	parts					428:432	aerial parts	421:432	aerial parts of C. divaricatum	421:450	The composition of hydroalcoholic extract from aerial parts of C. divaricatum was analyzed by HPLC-DAD-MSn, revealing the presence of numerous caffeic acid derivatives that were formerly unknown constituents of the plant.
31022860	8	19	theme	found	637:641	arg1	acids					655:659	commonly found chlorogenic acids	628:659	commonly found chlorogenic acids	628:659	In all, 17 compounds, including commonly found chlorogenic acids and rarely occurring butyryl and methylbutyryl tricaffeoylhexaric acids, were tentatively identified.
31022860	2	20	from	Parts	89:93	arg1	Contribution					127:138	Their Possible Contribution	112:138	Their Possible Contribution to the Biological Activity of the Plant	112:178	Revisited: Newly Identified Constituents from Aerial Parts of the Plant and Their Possible Contribution to the Biological Activity of the Plant.
31022860	2	20	from	Parts	89:93	arg1	Constituents					64:75	Newly Identified Constituents	47:75	Newly Identified Constituents from Aerial Parts of the Plant	47:106	Revisited: Newly Identified Constituents from Aerial Parts of the Plant and Their Possible Contribution to the Biological Activity of the Plant.
31022860	12	21	theme	pharmacological	1330:1344	arg1	activity					1346:1353	its pharmacological activity	1326:1353	its pharmacological activity	1326:1353	The newly identified constituents of the plant seem to be partly responsible for its pharmacological activity and elevate the value of C. divaricatum as a potential functional food.
31022860	2	22	theme	Identified	53:62	arg1	Constituents					64:75	Newly Identified Constituents	47:75	Newly Identified Constituents from Aerial Parts of the Plant	47:106	Revisited: Newly Identified Constituents from Aerial Parts of the Plant and Their Possible Contribution to the Biological Activity of the Plant.
31022860	9	23	theme	acid	866:869	arg1	isolation					833:841	the isolation	829:841	the isolation of 12-oxo-phytodienoic acid (12-OPDA), which is a newly identified constituent of the plant	829:933	Fractionation of lipophilic extract from cultivated shoots led to the isolation of 12-oxo-phytodienoic acid (12-OPDA), which is a newly identified constituent of the plant.
31022860	7	24	theme	acid	525:528	arg1	derivatives					530:540	numerous caffeic acid derivatives	508:540	numerous caffeic acid derivatives that were formerly unknown constituents of the plant	508:593	The composition of hydroalcoholic extract from aerial parts of C. divaricatum was analyzed by HPLC-DAD-MSn, revealing the presence of numerous caffeic acid derivatives that were formerly unknown constituents of the plant.
31022860	9	25	from	Fractionation	763:775	arg1	shoots					815:820	cultivated shoots	804:820	cultivated shoots	804:820	Fractionation of lipophilic extract from cultivated shoots led to the isolation of 12-oxo-phytodienoic acid (12-OPDA), which is a newly identified constituent of the plant.
31022860	8	26	theme	methylbutyryl	694:706	arg1	acids					727:731	rarely occurring butyryl and methylbutyryl tricaffeoylhexaric acids	665:731	acids	727:731	In all, 17 compounds, including commonly found chlorogenic acids and rarely occurring butyryl and methylbutyryl tricaffeoylhexaric acids, were tentatively identified.
31022860	10	27	theme	μM	989:990	arg1	concentrations					953:966	concentrations	953:966	concentrations of 0.5, 1.0, and 2.5 μM	953:990	The compound, at concentrations of 0.5, 1.0, and 2.5 μM, significantly reduced IL-8, IL-1β, TNFα, and CCL2 excretion by lipopolysaccharide (LPS)-stimulated human neutrophils.
31022860	7	28	theme	derivatives	530:540	arg1	presence					496:503	the presence	492:503	the presence of numerous caffeic acid derivatives that were formerly unknown constituents of the plant	492:593	The composition of hydroalcoholic extract from aerial parts of C. divaricatum was analyzed by HPLC-DAD-MSn, revealing the presence of numerous caffeic acid derivatives that were formerly unknown constituents of the plant.
31022860	7	29	theme	numerous	508:515	arg1	derivatives					530:540	numerous caffeic acid derivatives	508:540	numerous caffeic acid derivatives that were formerly unknown constituents of the plant	508:593	The composition of hydroalcoholic extract from aerial parts of C. divaricatum was analyzed by HPLC-DAD-MSn, revealing the presence of numerous caffeic acid derivatives that were formerly unknown constituents of the plant.
31022860	7	30	theme	aerial	421:426	arg1	parts					428:432	aerial parts	421:432	aerial parts of C. divaricatum	421:450	The composition of hydroalcoholic extract from aerial parts of C. divaricatum was analyzed by HPLC-DAD-MSn, revealing the presence of numerous caffeic acid derivatives that were formerly unknown constituents of the plant.
31022860	9	31	theme	12-oxo-phytodienoic	846:864	arg1	12-OPDA					872:878	12-OPDA	872:878	12-OPDA	872:878	Fractionation of lipophilic extract from cultivated shoots led to the isolation of 12-oxo-phytodienoic acid (12-OPDA), which is a newly identified constituent of the plant.
31022860	9	31	theme	12-oxo-phytodienoic	846:864	arg1	constituent					910:920	a newly identified constituent	891:920	a newly identified constituent of the plant	891:933	Fractionation of lipophilic extract from cultivated shoots led to the isolation of 12-oxo-phytodienoic acid (12-OPDA), which is a newly identified constituent of the plant.
31022860	9	31	theme	12-oxo-phytodienoic	846:864	arg1	acid					866:869	12-oxo-phytodienoic acid	846:869	12-oxo-phytodienoic acid (12-OPDA)	846:879	Fractionation of lipophilic extract from cultivated shoots led to the isolation of 12-oxo-phytodienoic acid (12-OPDA), which is a newly identified constituent of the plant.
31022860	7	32	theme	caffeic	517:523	arg1	derivatives					530:540	numerous caffeic acid derivatives	508:540	numerous caffeic acid derivatives that were formerly unknown constituents of the plant	508:593	The composition of hydroalcoholic extract from aerial parts of C. divaricatum was analyzed by HPLC-DAD-MSn, revealing the presence of numerous caffeic acid derivatives that were formerly unknown constituents of the plant.
31022860	5	33	theme	food	269:272	arg1	plant					274:278	a food plant	267:278	a food plant	267:278	has a long history of use as both a medicinal and a food plant.
31022860	5	33	theme	food	269:272	arg1	history					228:234	a long history	221:234	a long history of use	221:241	has a long history of use as both a medicinal and a food plant.
31022860	5	34	theme	long	223:226	arg1	plant					274:278	a food plant	267:278	a food plant	267:278	has a long history of use as both a medicinal and a food plant.
31022860	5	34	theme	long	223:226	arg1	history					228:234	a long history	221:234	a long history of use	221:241	has a long history of use as both a medicinal and a food plant.
31022860	5	34	theme	long	223:226	arg1	medicinal					253:261	a medicinal	251:261	a medicinal	251:261	has a long history of use as both a medicinal and a food plant.
31022860	7	35	from	parts	428:432	arg1	composition					378:388	The composition	374:388	The composition of hydroalcoholic extract from aerial parts of C. divaricatum	374:450	The composition of hydroalcoholic extract from aerial parts of C. divaricatum was analyzed by HPLC-DAD-MSn, revealing the presence of numerous caffeic acid derivatives that were formerly unknown constituents of the plant.
31022860	7	35	from	parts	428:432	arg1	extract					408:414	hydroalcoholic extract	393:414	hydroalcoholic extract from aerial parts of C. divaricatum	393:450	The composition of hydroalcoholic extract from aerial parts of C. divaricatum was analyzed by HPLC-DAD-MSn, revealing the presence of numerous caffeic acid derivatives that were formerly unknown constituents of the plant.
31022860	7	36	theme	extract	408:414	arg1	composition					378:388	The composition	374:388	The composition of hydroalcoholic extract from aerial parts of C. divaricatum	374:450	The composition of hydroalcoholic extract from aerial parts of C. divaricatum was analyzed by HPLC-DAD-MSn, revealing the presence of numerous caffeic acid derivatives that were formerly unknown constituents of the plant.
31022860	2	37	theme	Aerial	82:87	arg1	Parts					89:93	Aerial Parts	82:93	Aerial Parts of the Plant	82:106	Revisited: Newly Identified Constituents from Aerial Parts of the Plant and Their Possible Contribution to the Biological Activity of the Plant.
31022860	9	38	from	shoots	815:820	arg1	Fractionation					763:775	Fractionation	763:775	Fractionation of lipophilic extract from cultivated shoots	763:820	Fractionation of lipophilic extract from cultivated shoots led to the isolation of 12-oxo-phytodienoic acid (12-OPDA), which is a newly identified constituent of the plant.
31022860	9	38	from	shoots	815:820	arg1	extract					791:797	lipophilic extract	780:797	lipophilic extract from cultivated shoots	780:820	Fractionation of lipophilic extract from cultivated shoots led to the isolation of 12-oxo-phytodienoic acid (12-OPDA), which is a newly identified constituent of the plant.
31022860	12	39	theme	functional	1410:1419	arg1	food					1421:1424	a potential functional food	1398:1424	a potential functional food	1398:1424	The newly identified constituents of the plant seem to be partly responsible for its pharmacological activity and elevate the value of C. divaricatum as a potential functional food.
31022860	12	39	theme	functional	1410:1419	arg1	value					1371:1375	the value	1367:1375	the value of C. divaricatum	1367:1393	The newly identified constituents of the plant seem to be partly responsible for its pharmacological activity and elevate the value of C. divaricatum as a potential functional food.
31022860	6	40	theme	chemical	317:324	arg1	constituents					326:337	its chemical constituents	313:337	its chemical constituents	313:337	However, except for terpenoids, its chemical constituents have remained poorly investigated.
31022860	12	41	theme	plant	1286:1290	arg1	constituents					1266:1277	The newly identified constituents	1245:1277	The newly identified constituents of the plant	1245:1290	The newly identified constituents of the plant seem to be partly responsible for its pharmacological activity and elevate the value of C. divaricatum as a potential functional food.
31022860	12	41	theme	plant	1286:1290	arg1	responsible					1310:1320	responsible	1310:1320	responsible	1310:1320	The newly identified constituents of the plant seem to be partly responsible for its pharmacological activity and elevate the value of C. divaricatum as a potential functional food.
31022860	11	42	theme	Reactive	1111:1118	arg1	ROS					1136:1138	ROS	1136:1138	ROS	1136:1138	Reactive oxygen species (ROS) production induced by f-MLP was also significantly diminished in the neutrophils pretreated by 12-OPDA.
31022860	11	42	theme	Reactive	1111:1118	arg1	species					1127:1133	Reactive oxygen species	1111:1133	Reactive oxygen species (ROS) production induced by f-MLP	1111:1167	Reactive oxygen species (ROS) production induced by f-MLP was also significantly diminished in the neutrophils pretreated by 12-OPDA.
31022860	9	43	theme	lipophilic	780:789	arg1	extract					791:797	lipophilic extract	780:797	lipophilic extract from cultivated shoots	780:820	Fractionation of lipophilic extract from cultivated shoots led to the isolation of 12-oxo-phytodienoic acid (12-OPDA), which is a newly identified constituent of the plant.
31022860	7	44	theme	hydroalcoholic	393:406	arg1	extract					408:414	hydroalcoholic extract	393:414	hydroalcoholic extract from aerial parts of C. divaricatum	393:450	The composition of hydroalcoholic extract from aerial parts of C. divaricatum was analyzed by HPLC-DAD-MSn, revealing the presence of numerous caffeic acid derivatives that were formerly unknown constituents of the plant.
31022860	2	45	theme	Plant	174:178	arg1	Activity					158:165	the Biological Activity	143:165	the Biological Activity of the Plant	143:178	Revisited: Newly Identified Constituents from Aerial Parts of the Plant and Their Possible Contribution to the Biological Activity of the Plant.
31022860	9	46	theme	extract	791:797	arg1	Fractionation					763:775	Fractionation	763:775	Fractionation of lipophilic extract from cultivated shoots	763:820	Fractionation of lipophilic extract from cultivated shoots led to the isolation of 12-oxo-phytodienoic acid (12-OPDA), which is a newly identified constituent of the plant.
31022860	9	47	theme	identified	899:908	arg1	constituent					910:920	a newly identified constituent	891:920	a newly identified constituent of the plant	891:933	Fractionation of lipophilic extract from cultivated shoots led to the isolation of 12-oxo-phytodienoic acid (12-OPDA), which is a newly identified constituent of the plant.
31022860	9	47	theme	identified	899:908	arg1	acid					866:869	12-oxo-phytodienoic acid	846:869	12-oxo-phytodienoic acid (12-OPDA)	846:879	Fractionation of lipophilic extract from cultivated shoots led to the isolation of 12-oxo-phytodienoic acid (12-OPDA), which is a newly identified constituent of the plant.
31022860	12	48	theme	potential	1400:1408	arg1	food					1421:1424	a potential functional food	1398:1424	a potential functional food	1398:1424	The newly identified constituents of the plant seem to be partly responsible for its pharmacological activity and elevate the value of C. divaricatum as a potential functional food.
31022860	12	48	theme	potential	1400:1408	arg1	value					1371:1375	the value	1367:1375	the value of C. divaricatum	1367:1393	The newly identified constituents of the plant seem to be partly responsible for its pharmacological activity and elevate the value of C. divaricatum as a potential functional food.
31022860	8	49	theme	tricaffeoylhexaric	708:725	arg1	acids					727:731	rarely occurring butyryl and methylbutyryl tricaffeoylhexaric acids	665:731	acids	727:731	In all, 17 compounds, including commonly found chlorogenic acids and rarely occurring butyryl and methylbutyryl tricaffeoylhexaric acids, were tentatively identified.
29555681	4	0	theme	polyunsaturated	782:796	arg1	vLCPUFA					811:817	vLCPUFA	811:817	vLCPUFA	811:817	The four strains differed in composition of very-long-chain polyunsaturated fatty acids (vLCPUFA) in hepatic LDs.
29555681	4	0	theme	polyunsaturated	782:796	arg1	acids					804:808	very-long-chain polyunsaturated fatty acids	766:808	very-long-chain polyunsaturated fatty acids (vLCPUFA)	766:818	The four strains differed in composition of very-long-chain polyunsaturated fatty acids (vLCPUFA) in hepatic LDs.
29555681	6	1	from	phospholipids	1005:1017	arg1	mice					1042:1045	SA-ki and Pnpla3-/- mice	1022:1045	SA-ki and Pnpla3-/- mice	1022:1045	Conversely, vLCPUFAs were enriched in triglycerides and depleted from phospholipids in SA-ki and Pnpla3-/- mice.
29555681	3	2	theme	liver	493:497	arg1	lysates					499:505	liver lysates	493:505	liver lysates	493:505	To glean first insights into the physiological function of PNPLA3, we performed detailed lipidomic profiling of liver lysates and lipid droplets (LDs) from WT and Pnpla3-/- (KO) mice and from knock-in (ki) mice expressing either the 148M variant (IM-ki mice) or a variant (S47A) that renders the protein catalytically inactive (SA-ki mice).
29555681	0	3	theme	acids	92:96	arg1	transfer					64:71	transfer	64:71	transfer of essential fatty acids from triglycerides to phospholipids in hepatic lipid droplets	64:158	Patatin-like phospholipase domain–containing protein 3 promotes transfer of essential fatty acids from triglycerides to phospholipids in hepatic lipid droplets.
29555681	4	4	theme	very-long-chain	766:780	arg1	vLCPUFA					811:817	vLCPUFA	811:817	vLCPUFA	811:817	The four strains differed in composition of very-long-chain polyunsaturated fatty acids (vLCPUFA) in hepatic LDs.
29555681	4	4	theme	very-long-chain	766:780	arg1	acids					804:808	very-long-chain polyunsaturated fatty acids	766:808	very-long-chain polyunsaturated fatty acids (vLCPUFA)	766:818	The four strains differed in composition of very-long-chain polyunsaturated fatty acids (vLCPUFA) in hepatic LDs.
29555681	7	5	from	mice	1140:1143	arg1	droplets					1120:1127	droplets	1120:1127	droplets from IM-ki mice	1120:1143	Release of vLCPUFAs from hepatic LDs incubated ex vivo was increased in droplets from IM-ki mice and decreased from droplets isolated from Pnpla3-/- and SA-ki mice relative to those of WT mice.
29555681	3	6	theme	lysates	499:505	arg1	profiling					480:488	detailed lipidomic profiling	461:488	detailed lipidomic profiling of liver lysates and lipid droplets (LDs) from WT and Pnpla3-/- (KO) mice and from knock-in (ki) mice expressing either the 148M variant (IM-ki mice) or a variant (S47A) that renders the protein catalytically inactive (SA-ki mice)	461:719	To glean first insights into the physiological function of PNPLA3, we performed detailed lipidomic profiling of liver lysates and lipid droplets (LDs) from WT and Pnpla3-/- (KO) mice and from knock-in (ki) mice expressing either the 148M variant (IM-ki mice) or a variant (S47A) that renders the protein catalytically inactive (SA-ki mice).
29555681	9	7	from	variation	1563:1571	arg1	PNPLA3					1576:1581	PNPLA3	1576:1581	PNPLA3	1576:1581	Because SA-ki and IM-ki both cause FLD and yet have opposite effects on the lipidomic profile of LDs, we conclude that the FLD associated with genetic variation in PNPLA3 is not related to the enzyme's role in remodeling LD lipids.
29555681	4	8	theme	hepatic	823:829	arg1	LDs					831:833	hepatic LDs	823:833	hepatic LDs	823:833	The four strains differed in composition of very-long-chain polyunsaturated fatty acids (vLCPUFA) in hepatic LDs.
29555681	3	9	theme	IM-ki	628:632	arg1	mice					634:637	IM-ki mice	628:637	IM-ki mice	628:637	To glean first insights into the physiological function of PNPLA3, we performed detailed lipidomic profiling of liver lysates and lipid droplets (LDs) from WT and Pnpla3-/- (KO) mice and from knock-in (ki) mice expressing either the 148M variant (IM-ki mice) or a variant (S47A) that renders the protein catalytically inactive (SA-ki mice).
29555681	3	9	theme	IM-ki	628:632	arg1	variant					619:625	the 148M variant	610:625	the 148M variant (IM-ki mice)	610:638	To glean first insights into the physiological function of PNPLA3, we performed detailed lipidomic profiling of liver lysates and lipid droplets (LDs) from WT and Pnpla3-/- (KO) mice and from knock-in (ki) mice expressing either the 148M variant (IM-ki mice) or a variant (S47A) that renders the protein catalytically inactive (SA-ki mice).
29555681	3	10	theme	Pnpla3-/-	544:552	arg1	mice					559:562	WT and Pnpla3-/- (KO) mice	537:562	WT and Pnpla3-/- (KO) mice	537:562	To glean first insights into the physiological function of PNPLA3, we performed detailed lipidomic profiling of liver lysates and lipid droplets (LDs) from WT and Pnpla3-/- (KO) mice and from knock-in (ki) mice expressing either the 148M variant (IM-ki mice) or a variant (S47A) that renders the protein catalytically inactive (SA-ki mice).
29555681	3	10	theme	Pnpla3-/-	544:552	arg1	KO					555:556	KO	555:556	KO	555:556	To glean first insights into the physiological function of PNPLA3, we performed detailed lipidomic profiling of liver lysates and lipid droplets (LDs) from WT and Pnpla3-/- (KO) mice and from knock-in (ki) mice expressing either the 148M variant (IM-ki mice) or a variant (S47A) that renders the protein catalytically inactive (SA-ki mice).
29555681	9	11	theme	opposite	1464:1471	arg1	effects					1473:1479	opposite effects	1464:1479	opposite effects	1464:1479	Because SA-ki and IM-ki both cause FLD and yet have opposite effects on the lipidomic profile of LDs, we conclude that the FLD associated with genetic variation in PNPLA3 is not related to the enzyme's role in remodeling LD lipids.
29555681	5	12	theme	mice	856:859	arg1	LDs					843:845	the LDs	839:845	the LDs of IM-ki mice	839:859	In the LDs of IM-ki mice, vLCPUFAs were depleted from triglycerides and enriched in phospholipids.
29555681	5	13	theme	IM-ki	850:854	arg1	mice					856:859	IM-ki mice	850:859	IM-ki mice	850:859	In the LDs of IM-ki mice, vLCPUFAs were depleted from triglycerides and enriched in phospholipids.
29555681	4	14	theme	fatty	798:802	arg1	vLCPUFA					811:817	vLCPUFA	811:817	vLCPUFA	811:817	The four strains differed in composition of very-long-chain polyunsaturated fatty acids (vLCPUFA) in hepatic LDs.
29555681	4	14	theme	fatty	798:802	arg1	acids					804:808	very-long-chain polyunsaturated fatty acids	766:808	very-long-chain polyunsaturated fatty acids (vLCPUFA)	766:818	The four strains differed in composition of very-long-chain polyunsaturated fatty acids (vLCPUFA) in hepatic LDs.
29555681	3	15	theme	droplets	517:524	arg1	profiling					480:488	detailed lipidomic profiling	461:488	detailed lipidomic profiling of liver lysates and lipid droplets (LDs) from WT and Pnpla3-/- (KO) mice and from knock-in (ki) mice expressing either the 148M variant (IM-ki mice) or a variant (S47A) that renders the protein catalytically inactive (SA-ki mice)	461:719	To glean first insights into the physiological function of PNPLA3, we performed detailed lipidomic profiling of liver lysates and lipid droplets (LDs) from WT and Pnpla3-/- (KO) mice and from knock-in (ki) mice expressing either the 148M variant (IM-ki mice) or a variant (S47A) that renders the protein catalytically inactive (SA-ki mice).
29555681	3	16	from	mice	587:590	arg1	profiling					480:488	detailed lipidomic profiling	461:488	detailed lipidomic profiling of liver lysates and lipid droplets (LDs) from WT and Pnpla3-/- (KO) mice and from knock-in (ki) mice expressing either the 148M variant (IM-ki mice) or a variant (S47A) that renders the protein catalytically inactive (SA-ki mice)	461:719	To glean first insights into the physiological function of PNPLA3, we performed detailed lipidomic profiling of liver lysates and lipid droplets (LDs) from WT and Pnpla3-/- (KO) mice and from knock-in (ki) mice expressing either the 148M variant (IM-ki mice) or a variant (S47A) that renders the protein catalytically inactive (SA-ki mice).
29555681	3	16	from	mice	587:590	arg1	lysates					499:505	liver lysates	493:505	liver lysates	493:505	To glean first insights into the physiological function of PNPLA3, we performed detailed lipidomic profiling of liver lysates and lipid droplets (LDs) from WT and Pnpla3-/- (KO) mice and from knock-in (ki) mice expressing either the 148M variant (IM-ki mice) or a variant (S47A) that renders the protein catalytically inactive (SA-ki mice).
29555681	3	16	from	mice	587:590	arg1	droplets					517:524	lipid droplets	511:524	lipid droplets (LDs)	511:530	To glean first insights into the physiological function of PNPLA3, we performed detailed lipidomic profiling of liver lysates and lipid droplets (LDs) from WT and Pnpla3-/- (KO) mice and from knock-in (ki) mice expressing either the 148M variant (IM-ki mice) or a variant (S47A) that renders the protein catalytically inactive (SA-ki mice).
29555681	3	16	from	mice	587:590	arg1	LDs					527:529	LDs	527:529	LDs	527:529	To glean first insights into the physiological function of PNPLA3, we performed detailed lipidomic profiling of liver lysates and lipid droplets (LDs) from WT and Pnpla3-/- (KO) mice and from knock-in (ki) mice expressing either the 148M variant (IM-ki mice) or a variant (S47A) that renders the protein catalytically inactive (SA-ki mice).
29555681	4	17	from	composition	751:761	arg1	LDs					831:833	hepatic LDs	823:833	hepatic LDs	823:833	The four strains differed in composition of very-long-chain polyunsaturated fatty acids (vLCPUFA) in hepatic LDs.
29555681	7	18	theme	hepatic	1073:1079	arg1	LDs					1081:1083	hepatic LDs	1073:1083	hepatic LDs incubated ex vivo	1073:1101	Release of vLCPUFAs from hepatic LDs incubated ex vivo was increased in droplets from IM-ki mice and decreased from droplets isolated from Pnpla3-/- and SA-ki mice relative to those of WT mice.
29555681	7	19	theme	relative	1212:1219	arg1	mice					1207:1210	Pnpla3-/- and SA-ki mice	1187:1210	Pnpla3-/- and SA-ki mice relative to those of WT mice	1187:1239	Release of vLCPUFAs from hepatic LDs incubated ex vivo was increased in droplets from IM-ki mice and decreased from droplets isolated from Pnpla3-/- and SA-ki mice relative to those of WT mice.
29555681	2	20	theme	domain-containing	276:292	arg1	protein					294:300	patatin-like phospholipase domain-containing protein 3	249:302	patatin-like phospholipase domain-containing protein 3 (PNPLA3)	249:311	A missense variant (I148M) in patatin-like phospholipase domain-containing protein 3 (PNPLA3) confers susceptibility to FLD, although the mechanism is not known.
29555681	2	20	theme	domain-containing	276:292	arg1	PNPLA3					305:310	PNPLA3	305:310	PNPLA3	305:310	A missense variant (I148M) in patatin-like phospholipase domain-containing protein 3 (PNPLA3) confers susceptibility to FLD, although the mechanism is not known.
29555681	9	21	theme	remodeling	1622:1631	arg1	lipids					1636:1641	remodeling LD lipids	1622:1641	remodeling LD lipids	1622:1641	Because SA-ki and IM-ki both cause FLD and yet have opposite effects on the lipidomic profile of LDs, we conclude that the FLD associated with genetic variation in PNPLA3 is not related to the enzyme's role in remodeling LD lipids.
29555681	7	22	attach	isolated	1173:1180	arg1	mice					1207:1210	Pnpla3-/- and SA-ki mice	1187:1210	Pnpla3-/- and SA-ki mice relative to those of WT mice	1187:1239	Release of vLCPUFAs from hepatic LDs incubated ex vivo was increased in droplets from IM-ki mice and decreased from droplets isolated from Pnpla3-/- and SA-ki mice relative to those of WT mice.
29555681	7	22	attach	isolated	1173:1180	arg2	droplets					1164:1171	droplets	1164:1171	droplets isolated from Pnpla3-/- and SA-ki mice relative to those of WT mice	1164:1239	Release of vLCPUFAs from hepatic LDs incubated ex vivo was increased in droplets from IM-ki mice and decreased from droplets isolated from Pnpla3-/- and SA-ki mice relative to those of WT mice.
29555681	2	23	theme	phospholipase	262:274	arg1	protein					294:300	patatin-like phospholipase domain-containing protein 3	249:302	patatin-like phospholipase domain-containing protein 3 (PNPLA3)	249:311	A missense variant (I148M) in patatin-like phospholipase domain-containing protein 3 (PNPLA3) confers susceptibility to FLD, although the mechanism is not known.
29555681	2	23	theme	phospholipase	262:274	arg1	PNPLA3					305:310	PNPLA3	305:310	PNPLA3	305:310	A missense variant (I148M) in patatin-like phospholipase domain-containing protein 3 (PNPLA3) confers susceptibility to FLD, although the mechanism is not known.
29555681	7	24	theme	WT	1233:1234	arg1	mice					1236:1239	WT mice	1233:1239	WT mice	1233:1239	Release of vLCPUFAs from hepatic LDs incubated ex vivo was increased in droplets from IM-ki mice and decreased from droplets isolated from Pnpla3-/- and SA-ki mice relative to those of WT mice.
29555681	0	25	theme	phospholipase	13:25	arg1	protein					45:51	Patatin-like phospholipase domain–containing protein 3	0:53	Patatin-like phospholipase domain–containing protein 3	0:53	Patatin-like phospholipase domain–containing protein 3 promotes transfer of essential fatty acids from triglycerides to phospholipids in hepatic lipid droplets.
29555681	3	26	dep	inactive	699:706	arg1	mice					715:718	SA-ki mice	709:718	SA-ki mice	709:718	To glean first insights into the physiological function of PNPLA3, we performed detailed lipidomic profiling of liver lysates and lipid droplets (LDs) from WT and Pnpla3-/- (KO) mice and from knock-in (ki) mice expressing either the 148M variant (IM-ki mice) or a variant (S47A) that renders the protein catalytically inactive (SA-ki mice).
29555681	9	27	theme	LDs	1509:1511	arg1	profile					1498:1504	the lipidomic profile	1484:1504	the lipidomic profile of LDs	1484:1511	Because SA-ki and IM-ki both cause FLD and yet have opposite effects on the lipidomic profile of LDs, we conclude that the FLD associated with genetic variation in PNPLA3 is not related to the enzyme's role in remodeling LD lipids.
29555681	8	28	theme	physiological	1252:1264	arg1	role					1266:1269	the physiological role	1248:1269	the physiological role of PNPLA3	1248:1279	Thus, the physiological role of PNPLA3 appears to be to remodel triglycerides and phospholipids in LDs, perhaps to accommodate changes in LD size in response to feeding.
29555681	2	29	from	variant	230:236	arg1	protein					294:300	patatin-like phospholipase domain-containing protein 3	249:302	patatin-like phospholipase domain-containing protein 3 (PNPLA3)	249:311	A missense variant (I148M) in patatin-like phospholipase domain-containing protein 3 (PNPLA3) confers susceptibility to FLD, although the mechanism is not known.
29555681	2	29	from	variant	230:236	arg1	PNPLA3					305:310	PNPLA3	305:310	PNPLA3	305:310	A missense variant (I148M) in patatin-like phospholipase domain-containing protein 3 (PNPLA3) confers susceptibility to FLD, although the mechanism is not known.
29555681	0	30	theme	Patatin-like	0:11	arg1	protein					45:51	Patatin-like phospholipase domain–containing protein 3	0:53	Patatin-like phospholipase domain–containing protein 3	0:53	Patatin-like phospholipase domain–containing protein 3 promotes transfer of essential fatty acids from triglycerides to phospholipids in hepatic lipid droplets.
29555681	3	31	from	profiling	480:488	arg1	mice					587:590	knock-in (ki) mice	573:590	knock-in (ki) mice expressing either the 148M variant (IM-ki mice) or a variant (S47A) that renders the protein catalytically inactive (SA-ki mice)	573:719	To glean first insights into the physiological function of PNPLA3, we performed detailed lipidomic profiling of liver lysates and lipid droplets (LDs) from WT and Pnpla3-/- (KO) mice and from knock-in (ki) mice expressing either the 148M variant (IM-ki mice) or a variant (S47A) that renders the protein catalytically inactive (SA-ki mice).
29555681	3	31	from	profiling	480:488	arg1	mice					559:562	WT and Pnpla3-/- (KO) mice	537:562	WT and Pnpla3-/- (KO) mice	537:562	To glean first insights into the physiological function of PNPLA3, we performed detailed lipidomic profiling of liver lysates and lipid droplets (LDs) from WT and Pnpla3-/- (KO) mice and from knock-in (ki) mice expressing either the 148M variant (IM-ki mice) or a variant (S47A) that renders the protein catalytically inactive (SA-ki mice).
29555681	3	31	from	profiling	480:488	arg1	KO					555:556	KO	555:556	KO	555:556	To glean first insights into the physiological function of PNPLA3, we performed detailed lipidomic profiling of liver lysates and lipid droplets (LDs) from WT and Pnpla3-/- (KO) mice and from knock-in (ki) mice expressing either the 148M variant (IM-ki mice) or a variant (S47A) that renders the protein catalytically inactive (SA-ki mice).
29555681	3	32	theme	knock-in	573:580	arg1	mice					587:590	knock-in (ki) mice	573:590	knock-in (ki) mice expressing either the 148M variant (IM-ki mice) or a variant (S47A) that renders the protein catalytically inactive (SA-ki mice)	573:719	To glean first insights into the physiological function of PNPLA3, we performed detailed lipidomic profiling of liver lysates and lipid droplets (LDs) from WT and Pnpla3-/- (KO) mice and from knock-in (ki) mice expressing either the 148M variant (IM-ki mice) or a variant (S47A) that renders the protein catalytically inactive (SA-ki mice).
29555681	9	33	from	role	1614:1617	arg1	lipids					1636:1641	remodeling LD lipids	1622:1641	remodeling LD lipids	1622:1641	Because SA-ki and IM-ki both cause FLD and yet have opposite effects on the lipidomic profile of LDs, we conclude that the FLD associated with genetic variation in PNPLA3 is not related to the enzyme's role in remodeling LD lipids.
29555681	3	34	theme	physiological	414:426	arg1	function					428:435	the physiological function	410:435	the physiological function of PNPLA3	410:445	To glean first insights into the physiological function of PNPLA3, we performed detailed lipidomic profiling of liver lysates and lipid droplets (LDs) from WT and Pnpla3-/- (KO) mice and from knock-in (ki) mice expressing either the 148M variant (IM-ki mice) or a variant (S47A) that renders the protein catalytically inactive (SA-ki mice).
29555681	9	35	contain	have	1459:1462	arg2	effects					1473:1479	opposite effects	1464:1479	opposite effects	1464:1479	Because SA-ki and IM-ki both cause FLD and yet have opposite effects on the lipidomic profile of LDs, we conclude that the FLD associated with genetic variation in PNPLA3 is not related to the enzyme's role in remodeling LD lipids.
29555681	9	35	contain	have	1459:1462	arg1	SA-ki					1420:1424	SA-ki	1420:1424	SA-ki	1420:1424	Because SA-ki and IM-ki both cause FLD and yet have opposite effects on the lipidomic profile of LDs, we conclude that the FLD associated with genetic variation in PNPLA3 is not related to the enzyme's role in remodeling LD lipids.
29555681	0	36	theme	lipid	145:149	arg1	droplets					151:158	hepatic lipid droplets	137:158	hepatic lipid droplets	137:158	Patatin-like phospholipase domain–containing protein 3 promotes transfer of essential fatty acids from triglycerides to phospholipids in hepatic lipid droplets.
29555681	8	37	from	phospholipids	1324:1336	arg1	LDs					1341:1343	LDs	1341:1343	LDs	1341:1343	Thus, the physiological role of PNPLA3 appears to be to remodel triglycerides and phospholipids in LDs, perhaps to accommodate changes in LD size in response to feeding.
29555681	8	38	from	triglycerides	1306:1318	arg1	LDs					1341:1343	LDs	1341:1343	LDs	1341:1343	Thus, the physiological role of PNPLA3 appears to be to remodel triglycerides and phospholipids in LDs, perhaps to accommodate changes in LD size in response to feeding.
29555681	1	39	theme	burgeoning	192:201	arg1	disease					173:179	Fatty liver disease	161:179	Fatty liver disease (FLD)	161:185	Fatty liver disease (FLD) is a burgeoning health problem.
29555681	1	39	theme	burgeoning	192:201	arg1	problem					210:216	a burgeoning health problem	190:216	a burgeoning health problem	190:216	Fatty liver disease (FLD) is a burgeoning health problem.
29555681	0	40	theme	domain–containing	27:43	arg1	protein					45:51	Patatin-like phospholipase domain–containing protein 3	0:53	Patatin-like phospholipase domain–containing protein 3	0:53	Patatin-like phospholipase domain–containing protein 3 promotes transfer of essential fatty acids from triglycerides to phospholipids in hepatic lipid droplets.
29555681	0	41	theme	hepatic	137:143	arg1	droplets					151:158	hepatic lipid droplets	137:158	hepatic lipid droplets	137:158	Patatin-like phospholipase domain–containing protein 3 promotes transfer of essential fatty acids from triglycerides to phospholipids in hepatic lipid droplets.
29555681	3	42	theme	WT	537:538	arg1	mice					559:562	WT and Pnpla3-/- (KO) mice	537:562	WT and Pnpla3-/- (KO) mice	537:562	To glean first insights into the physiological function of PNPLA3, we performed detailed lipidomic profiling of liver lysates and lipid droplets (LDs) from WT and Pnpla3-/- (KO) mice and from knock-in (ki) mice expressing either the 148M variant (IM-ki mice) or a variant (S47A) that renders the protein catalytically inactive (SA-ki mice).
29555681	3	42	theme	WT	537:538	arg1	KO					555:556	KO	555:556	KO	555:556	To glean first insights into the physiological function of PNPLA3, we performed detailed lipidomic profiling of liver lysates and lipid droplets (LDs) from WT and Pnpla3-/- (KO) mice and from knock-in (ki) mice expressing either the 148M variant (IM-ki mice) or a variant (S47A) that renders the protein catalytically inactive (SA-ki mice).
29555681	1	43	theme	health	203:208	arg1	disease					173:179	Fatty liver disease	161:179	Fatty liver disease (FLD)	161:185	Fatty liver disease (FLD) is a burgeoning health problem.
29555681	1	43	theme	health	203:208	arg1	problem					210:216	a burgeoning health problem	190:216	a burgeoning health problem	190:216	Fatty liver disease (FLD) is a burgeoning health problem.
29555681	6	44	theme	Pnpla3-/-	1032:1040	arg1	mice					1042:1045	SA-ki and Pnpla3-/- mice	1022:1045	SA-ki and Pnpla3-/- mice	1022:1045	Conversely, vLCPUFAs were enriched in triglycerides and depleted from phospholipids in SA-ki and Pnpla3-/- mice.
29555681	9	45	theme	genetic	1555:1561	arg1	variation					1563:1571	genetic variation	1555:1571	genetic variation in PNPLA3	1555:1581	Because SA-ki and IM-ki both cause FLD and yet have opposite effects on the lipidomic profile of LDs, we conclude that the FLD associated with genetic variation in PNPLA3 is not related to the enzyme's role in remodeling LD lipids.
29555681	3	46	from	mice	559:562	arg1	profiling					480:488	detailed lipidomic profiling	461:488	detailed lipidomic profiling of liver lysates and lipid droplets (LDs) from WT and Pnpla3-/- (KO) mice and from knock-in (ki) mice expressing either the 148M variant (IM-ki mice) or a variant (S47A) that renders the protein catalytically inactive (SA-ki mice)	461:719	To glean first insights into the physiological function of PNPLA3, we performed detailed lipidomic profiling of liver lysates and lipid droplets (LDs) from WT and Pnpla3-/- (KO) mice and from knock-in (ki) mice expressing either the 148M variant (IM-ki mice) or a variant (S47A) that renders the protein catalytically inactive (SA-ki mice).
29555681	3	46	from	mice	559:562	arg1	lysates					499:505	liver lysates	493:505	liver lysates	493:505	To glean first insights into the physiological function of PNPLA3, we performed detailed lipidomic profiling of liver lysates and lipid droplets (LDs) from WT and Pnpla3-/- (KO) mice and from knock-in (ki) mice expressing either the 148M variant (IM-ki mice) or a variant (S47A) that renders the protein catalytically inactive (SA-ki mice).
29555681	3	46	from	mice	559:562	arg1	droplets					517:524	lipid droplets	511:524	lipid droplets (LDs)	511:530	To glean first insights into the physiological function of PNPLA3, we performed detailed lipidomic profiling of liver lysates and lipid droplets (LDs) from WT and Pnpla3-/- (KO) mice and from knock-in (ki) mice expressing either the 148M variant (IM-ki mice) or a variant (S47A) that renders the protein catalytically inactive (SA-ki mice).
29555681	3	46	from	mice	559:562	arg1	LDs					527:529	LDs	527:529	LDs	527:529	To glean first insights into the physiological function of PNPLA3, we performed detailed lipidomic profiling of liver lysates and lipid droplets (LDs) from WT and Pnpla3-/- (KO) mice and from knock-in (ki) mice expressing either the 148M variant (IM-ki mice) or a variant (S47A) that renders the protein catalytically inactive (SA-ki mice).
29555681	3	47	theme	PNPLA3	440:445	arg1	function					428:435	the physiological function	410:435	the physiological function of PNPLA3	410:445	To glean first insights into the physiological function of PNPLA3, we performed detailed lipidomic profiling of liver lysates and lipid droplets (LDs) from WT and Pnpla3-/- (KO) mice and from knock-in (ki) mice expressing either the 148M variant (IM-ki mice) or a variant (S47A) that renders the protein catalytically inactive (SA-ki mice).
29555681	7	48	theme	vLCPUFAs	1059:1066	arg1	Release					1048:1054	Release	1048:1054	Release of vLCPUFAs from hepatic LDs incubated ex vivo	1048:1101	Release of vLCPUFAs from hepatic LDs incubated ex vivo was increased in droplets from IM-ki mice and decreased from droplets isolated from Pnpla3-/- and SA-ki mice relative to those of WT mice.
29555681	3	49	theme	ki	583:584	arg1	mice					587:590	knock-in (ki) mice	573:590	knock-in (ki) mice expressing either the 148M variant (IM-ki mice) or a variant (S47A) that renders the protein catalytically inactive (SA-ki mice)	573:719	To glean first insights into the physiological function of PNPLA3, we performed detailed lipidomic profiling of liver lysates and lipid droplets (LDs) from WT and Pnpla3-/- (KO) mice and from knock-in (ki) mice expressing either the 148M variant (IM-ki mice) or a variant (S47A) that renders the protein catalytically inactive (SA-ki mice).
29555681	8	50	theme	PNPLA3	1274:1279	arg1	role					1266:1269	the physiological role	1248:1269	the physiological role of PNPLA3	1248:1279	Thus, the physiological role of PNPLA3 appears to be to remodel triglycerides and phospholipids in LDs, perhaps to accommodate changes in LD size in response to feeding.
29555681	7	51	theme	SA-ki	1201:1205	arg1	mice					1207:1210	Pnpla3-/- and SA-ki mice	1187:1210	Pnpla3-/- and SA-ki mice relative to those of WT mice	1187:1239	Release of vLCPUFAs from hepatic LDs incubated ex vivo was increased in droplets from IM-ki mice and decreased from droplets isolated from Pnpla3-/- and SA-ki mice relative to those of WT mice.
29555681	8	52	from	changes	1369:1375	arg1	size					1383:1386	LD size	1380:1386	LD size	1380:1386	Thus, the physiological role of PNPLA3 appears to be to remodel triglycerides and phospholipids in LDs, perhaps to accommodate changes in LD size in response to feeding.
29555681	8	52	from	changes	1369:1375	arg1	response					1391:1398	response	1391:1398	response to feeding	1391:1409	Thus, the physiological role of PNPLA3 appears to be to remodel triglycerides and phospholipids in LDs, perhaps to accommodate changes in LD size in response to feeding.
29555681	7	53	theme	IM-ki	1134:1138	arg1	mice					1140:1143	IM-ki mice	1134:1143	IM-ki mice	1134:1143	Release of vLCPUFAs from hepatic LDs incubated ex vivo was increased in droplets from IM-ki mice and decreased from droplets isolated from Pnpla3-/- and SA-ki mice relative to those of WT mice.
29555681	2	54	theme	missense	221:228	arg1	variant					230:236	A missense variant	219:236	A missense variant (I148M) in patatin-like phospholipase domain-containing protein 3 (PNPLA3)	219:311	A missense variant (I148M) in patatin-like phospholipase domain-containing protein 3 (PNPLA3) confers susceptibility to FLD, although the mechanism is not known.
29555681	2	54	theme	missense	221:228	arg1	I148M					239:243	I148M	239:243	I148M	239:243	A missense variant (I148M) in patatin-like phospholipase domain-containing protein 3 (PNPLA3) confers susceptibility to FLD, although the mechanism is not known.
29555681	7	55	from	LDs	1081:1083	arg1	Release					1048:1054	Release	1048:1054	Release of vLCPUFAs from hepatic LDs incubated ex vivo	1048:1101	Release of vLCPUFAs from hepatic LDs incubated ex vivo was increased in droplets from IM-ki mice and decreased from droplets isolated from Pnpla3-/- and SA-ki mice relative to those of WT mice.
29555681	3	56	theme	first	390:394	arg1	insights					396:403	first insights	390:403	first insights into the physiological function of PNPLA3	390:445	To glean first insights into the physiological function of PNPLA3, we performed detailed lipidomic profiling of liver lysates and lipid droplets (LDs) from WT and Pnpla3-/- (KO) mice and from knock-in (ki) mice expressing either the 148M variant (IM-ki mice) or a variant (S47A) that renders the protein catalytically inactive (SA-ki mice).
29555681	3	57	theme	148M	614:617	arg1	mice					634:637	IM-ki mice	628:637	IM-ki mice	628:637	To glean first insights into the physiological function of PNPLA3, we performed detailed lipidomic profiling of liver lysates and lipid droplets (LDs) from WT and Pnpla3-/- (KO) mice and from knock-in (ki) mice expressing either the 148M variant (IM-ki mice) or a variant (S47A) that renders the protein catalytically inactive (SA-ki mice).
29555681	3	57	theme	148M	614:617	arg1	variant					619:625	the 148M variant	610:625	the 148M variant (IM-ki mice)	610:638	To glean first insights into the physiological function of PNPLA3, we performed detailed lipidomic profiling of liver lysates and lipid droplets (LDs) from WT and Pnpla3-/- (KO) mice and from knock-in (ki) mice expressing either the 148M variant (IM-ki mice) or a variant (S47A) that renders the protein catalytically inactive (SA-ki mice).
29555681	4	58	theme	acids	804:808	arg1	composition					751:761	composition	751:761	composition of very-long-chain polyunsaturated fatty acids (vLCPUFA) in hepatic LDs	751:833	The four strains differed in composition of very-long-chain polyunsaturated fatty acids (vLCPUFA) in hepatic LDs.
29555681	0	59	from	phospholipids	120:132	arg1	droplets					151:158	hepatic lipid droplets	137:158	hepatic lipid droplets	137:158	Patatin-like phospholipase domain–containing protein 3 promotes transfer of essential fatty acids from triglycerides to phospholipids in hepatic lipid droplets.
29555681	2	60	theme	patatin-like	249:260	arg1	protein					294:300	patatin-like phospholipase domain-containing protein 3	249:302	patatin-like phospholipase domain-containing protein 3 (PNPLA3)	249:311	A missense variant (I148M) in patatin-like phospholipase domain-containing protein 3 (PNPLA3) confers susceptibility to FLD, although the mechanism is not known.
29555681	2	60	theme	patatin-like	249:260	arg1	PNPLA3					305:310	PNPLA3	305:310	PNPLA3	305:310	A missense variant (I148M) in patatin-like phospholipase domain-containing protein 3 (PNPLA3) confers susceptibility to FLD, although the mechanism is not known.
29555681	3	61	theme	detailed	461:468	arg1	profiling					480:488	detailed lipidomic profiling	461:488	detailed lipidomic profiling of liver lysates and lipid droplets (LDs) from WT and Pnpla3-/- (KO) mice and from knock-in (ki) mice expressing either the 148M variant (IM-ki mice) or a variant (S47A) that renders the protein catalytically inactive (SA-ki mice)	461:719	To glean first insights into the physiological function of PNPLA3, we performed detailed lipidomic profiling of liver lysates and lipid droplets (LDs) from WT and Pnpla3-/- (KO) mice and from knock-in (ki) mice expressing either the 148M variant (IM-ki mice) or a variant (S47A) that renders the protein catalytically inactive (SA-ki mice).
29555681	6	62	theme	SA-ki	1022:1026	arg1	mice					1042:1045	SA-ki and Pnpla3-/- mice	1022:1045	SA-ki and Pnpla3-/- mice	1022:1045	Conversely, vLCPUFAs were enriched in triglycerides and depleted from phospholipids in SA-ki and Pnpla3-/- mice.
29555681	1	63	theme	liver	167:171	arg1	problem					210:216	a burgeoning health problem	190:216	a burgeoning health problem	190:216	Fatty liver disease (FLD) is a burgeoning health problem.
29555681	1	63	theme	liver	167:171	arg1	FLD					182:184	FLD	182:184	FLD	182:184	Fatty liver disease (FLD) is a burgeoning health problem.
29555681	1	63	theme	liver	167:171	arg1	disease					173:179	Fatty liver disease	161:179	Fatty liver disease (FLD)	161:185	Fatty liver disease (FLD) is a burgeoning health problem.
29555681	9	64	theme	LD	1633:1634	arg1	lipids					1636:1641	remodeling LD lipids	1622:1641	remodeling LD lipids	1622:1641	Because SA-ki and IM-ki both cause FLD and yet have opposite effects on the lipidomic profile of LDs, we conclude that the FLD associated with genetic variation in PNPLA3 is not related to the enzyme's role in remodeling LD lipids.
29555681	0	65	theme	fatty	86:90	arg1	acids					92:96	essential fatty acids	76:96	essential fatty acids	76:96	Patatin-like phospholipase domain–containing protein 3 promotes transfer of essential fatty acids from triglycerides to phospholipids in hepatic lipid droplets.
29555681	0	66	from	triglycerides	103:115	arg1	transfer					64:71	transfer	64:71	transfer of essential fatty acids from triglycerides to phospholipids in hepatic lipid droplets	64:158	Patatin-like phospholipase domain–containing protein 3 promotes transfer of essential fatty acids from triglycerides to phospholipids in hepatic lipid droplets.
29555681	9	67	theme	lipidomic	1488:1496	arg1	profile					1498:1504	the lipidomic profile	1484:1504	the lipidomic profile of LDs	1484:1511	Because SA-ki and IM-ki both cause FLD and yet have opposite effects on the lipidomic profile of LDs, we conclude that the FLD associated with genetic variation in PNPLA3 is not related to the enzyme's role in remodeling LD lipids.
29555681	1	68	theme	Fatty	161:165	arg1	problem					210:216	a burgeoning health problem	190:216	a burgeoning health problem	190:216	Fatty liver disease (FLD) is a burgeoning health problem.
29555681	1	68	theme	Fatty	161:165	arg1	FLD					182:184	FLD	182:184	FLD	182:184	Fatty liver disease (FLD) is a burgeoning health problem.
29555681	1	68	theme	Fatty	161:165	arg1	disease					173:179	Fatty liver disease	161:179	Fatty liver disease (FLD)	161:185	Fatty liver disease (FLD) is a burgeoning health problem.
29555681	3	69	theme	lipidomic	470:478	arg1	profiling					480:488	detailed lipidomic profiling	461:488	detailed lipidomic profiling of liver lysates and lipid droplets (LDs) from WT and Pnpla3-/- (KO) mice and from knock-in (ki) mice expressing either the 148M variant (IM-ki mice) or a variant (S47A) that renders the protein catalytically inactive (SA-ki mice)	461:719	To glean first insights into the physiological function of PNPLA3, we performed detailed lipidomic profiling of liver lysates and lipid droplets (LDs) from WT and Pnpla3-/- (KO) mice and from knock-in (ki) mice expressing either the 148M variant (IM-ki mice) or a variant (S47A) that renders the protein catalytically inactive (SA-ki mice).
29555681	0	70	theme	essential	76:84	arg1	acids					92:96	essential fatty acids	76:96	essential fatty acids	76:96	Patatin-like phospholipase domain–containing protein 3 promotes transfer of essential fatty acids from triglycerides to phospholipids in hepatic lipid droplets.
29555681	8	71	theme	LD	1380:1381	arg1	size					1383:1386	LD size	1380:1386	LD size	1380:1386	Thus, the physiological role of PNPLA3 appears to be to remodel triglycerides and phospholipids in LDs, perhaps to accommodate changes in LD size in response to feeding.
29555681	7	72	theme	Pnpla3-/-	1187:1195	arg1	mice					1207:1210	Pnpla3-/- and SA-ki mice	1187:1210	Pnpla3-/- and SA-ki mice relative to those of WT mice	1187:1239	Release of vLCPUFAs from hepatic LDs incubated ex vivo was increased in droplets from IM-ki mice and decreased from droplets isolated from Pnpla3-/- and SA-ki mice relative to those of WT mice.
29555681	3	73	theme	lipid	511:515	arg1	LDs					527:529	LDs	527:529	LDs	527:529	To glean first insights into the physiological function of PNPLA3, we performed detailed lipidomic profiling of liver lysates and lipid droplets (LDs) from WT and Pnpla3-/- (KO) mice and from knock-in (ki) mice expressing either the 148M variant (IM-ki mice) or a variant (S47A) that renders the protein catalytically inactive (SA-ki mice).
29555681	3	73	theme	lipid	511:515	arg1	droplets					517:524	lipid droplets	511:524	lipid droplets (LDs)	511:530	To glean first insights into the physiological function of PNPLA3, we performed detailed lipidomic profiling of liver lysates and lipid droplets (LDs) from WT and Pnpla3-/- (KO) mice and from knock-in (ki) mice expressing either the 148M variant (IM-ki mice) or a variant (S47A) that renders the protein catalytically inactive (SA-ki mice).
29555681	3	74	theme	SA-ki	709:713	arg1	mice					715:718	SA-ki mice	709:718	SA-ki mice	709:718	To glean first insights into the physiological function of PNPLA3, we performed detailed lipidomic profiling of liver lysates and lipid droplets (LDs) from WT and Pnpla3-/- (KO) mice and from knock-in (ki) mice expressing either the 148M variant (IM-ki mice) or a variant (S47A) that renders the protein catalytically inactive (SA-ki mice).
31117738	7	0	theme	tri-saccharide-based	1202:1221	arg1	binders					1223:1229	the best di- or tri-saccharide-based binders	1186:1229	the best di- or tri-saccharide-based binders reported to date	1186:1246	Hence, a spacer length of 4 ethylene glycol and a surface density of 15% resulted in a dissociation constant Kd,multi of 1.3 × 10-13 M, on par with the best di- or tri-saccharide-based binders reported to date, whereas a density of 20% demonstrated complete resistance to hemagglutinin binding.
31117738	6	1	theme	ligands	1029:1035	arg1	density					988:994	density	988:994	density	988:994	These surfaces' affinity for the lectin hemagglutinin revealed a strong dependence of the affinity on the presentation, density, and mobility of the sialic acid ligands.
31117738	6	1	theme	ligands	1029:1035	arg1	mobility					1001:1008	mobility	1001:1008	mobility	1001:1008	These surfaces' affinity for the lectin hemagglutinin revealed a strong dependence of the affinity on the presentation, density, and mobility of the sialic acid ligands.
31117738	6	1	theme	ligands	1029:1035	arg1	presentation					974:985	presentation	974:985	presentation	974:985	These surfaces' affinity for the lectin hemagglutinin revealed a strong dependence of the affinity on the presentation, density, and mobility of the sialic acid ligands.
31117738	9	2	theme	fundamental	1659:1669	arg1	studies					1683:1689	both fundamental and applied studies	1654:1689	both fundamental and applied studies	1654:1689	The results have a direct bearing on biological cell surface multivalent recognition involving lipid bilayers and may guide the design of model surfaces and sensors for both fundamental and applied studies.
31117738	0	3	with	Monolayers	40:49	arg1	Affinity					104:111	Clustering-Dependent Lectin Affinity	76:111	Clustering-Dependent Lectin Affinity	76:111	Lipid Bilayer-like Mixed Self-Assembled Monolayers with Strong Mobility and Clustering-Dependent Lectin Affinity.
31117738	0	3	with	Monolayers	40:49	arg1	Mobility					63:70	Strong Mobility	56:70	Strong Mobility	56:70	Lipid Bilayer-like Mixed Self-Assembled Monolayers with Strong Mobility and Clustering-Dependent Lectin Affinity.
31117738	9	4	theme	surface	1538:1544	arg1	recognition					1558:1568	biological cell surface multivalent recognition	1522:1568	biological cell surface multivalent recognition involving lipid bilayers	1522:1593	The results have a direct bearing on biological cell surface multivalent recognition involving lipid bilayers and may guide the design of model surfaces and sensors for both fundamental and applied studies.
31117738	0	5	theme	Lectin	97:102	arg1	Affinity					104:111	Clustering-Dependent Lectin Affinity	76:111	Clustering-Dependent Lectin Affinity	76:111	Lipid Bilayer-like Mixed Self-Assembled Monolayers with Strong Mobility and Clustering-Dependent Lectin Affinity.
31117738	4	6	from	surface	557:563	arg1	mixture					570:576	a mixture	568:576	a mixture of sialic acid- and hydroxy-terminated benzamidine amphiphiles	568:639	Mixed rSAMs were prepared in water by simple immersion of a negatively charged surface in a mixture of sialic acid- and hydroxy-terminated benzamidine amphiphiles.
31117738	8	7	theme	same	1470:1473	arg1	interval					1475:1482	the same interval	1466:1482	the same interval	1466:1482	These results correlated with ligand mobility measured by fluorescence recovery after photobleaching which showed a dramatic drop in the same interval.
31117738	4	8	theme	amphiphiles	629:639	arg1	mixture					570:576	a mixture	568:576	a mixture of sialic acid- and hydroxy-terminated benzamidine amphiphiles	568:639	Mixed rSAMs were prepared in water by simple immersion of a negatively charged surface in a mixture of sialic acid- and hydroxy-terminated benzamidine amphiphiles.
31117738	3	9	theme	reversible	401:410	arg1	rSAMs					439:443	rSAMs	439:443	rSAMs	439:443	We here present a series of mixed reversible self-assembled monolayers (rSAMs) that addresses this deficiency.
31117738	3	9	theme	reversible	401:410	arg1	monolayers					427:436	mixed reversible self-assembled monolayers	395:436	mixed reversible self-assembled monolayers (rSAMs)	395:444	We here present a series of mixed reversible self-assembled monolayers (rSAMs) that addresses this deficiency.
31117738	4	10	theme	surface	557:563	arg1	immersion					523:531	simple immersion	516:531	simple immersion of a negatively charged surface in a mixture of sialic acid- and hydroxy-terminated benzamidine amphiphiles	516:639	Mixed rSAMs were prepared in water by simple immersion of a negatively charged surface in a mixture of sialic acid- and hydroxy-terminated benzamidine amphiphiles.
31117738	5	11	theme	thickness	736:744	arg1	information					746:756	film thickness information	731:756	film thickness information (atomic force microscopy, ellipsometry)	731:796	Surface compositions derived from infrared reflection-absorption spectroscopy (IRAS) and film thickness information (atomic force microscopy, ellipsometry) suggest the latter to be statistically incorporated in the monolayer.
31117738	9	12	theme	model	1623:1627	arg1	surfaces					1629:1636	model surfaces	1623:1636	model surfaces	1623:1636	The results have a direct bearing on biological cell surface multivalent recognition involving lipid bilayers and may guide the design of model surfaces and sensors for both fundamental and applied studies.
31117738	9	13	theme	lipid	1580:1584	arg1	bilayers					1586:1593	lipid bilayers	1580:1593	lipid bilayers	1580:1593	The results have a direct bearing on biological cell surface multivalent recognition involving lipid bilayers and may guide the design of model surfaces and sensors for both fundamental and applied studies.
31117738	3	14	theme	self-assembled	412:425	arg1	rSAMs					439:443	rSAMs	439:443	rSAMs	439:443	We here present a series of mixed reversible self-assembled monolayers (rSAMs) that addresses this deficiency.
31117738	3	14	theme	self-assembled	412:425	arg1	monolayers					427:436	mixed reversible self-assembled monolayers	395:436	mixed reversible self-assembled monolayers (rSAMs)	395:444	We here present a series of mixed reversible self-assembled monolayers (rSAMs) that addresses this deficiency.
31117738	7	15	theme	glycol	1075:1080	arg1	length					1054:1059	a spacer length	1045:1059	a spacer length of 4 ethylene glycol	1045:1080	Hence, a spacer length of 4 ethylene glycol and a surface density of 15% resulted in a dissociation constant Kd,multi of 1.3 × 10-13 M, on par with the best di- or tri-saccharide-based binders reported to date, whereas a density of 20% demonstrated complete resistance to hemagglutinin binding.
31117738	7	15	theme	glycol	1075:1080	arg1	density					1096:1102	a surface density	1086:1102	a surface density of 15%	1086:1109	Hence, a spacer length of 4 ethylene glycol and a surface density of 15% resulted in a dissociation constant Kd,multi of 1.3 × 10-13 M, on par with the best di- or tri-saccharide-based binders reported to date, whereas a density of 20% demonstrated complete resistance to hemagglutinin binding.
31117738	5	16	theme	infrared	676:683	arg1	IRAS					721:724	IRAS	721:724	IRAS	721:724	Surface compositions derived from infrared reflection-absorption spectroscopy (IRAS) and film thickness information (atomic force microscopy, ellipsometry) suggest the latter to be statistically incorporated in the monolayer.
31117738	5	16	theme	infrared	676:683	arg1	spectroscopy					707:718	infrared reflection-absorption spectroscopy	676:718	infrared reflection-absorption spectroscopy (IRAS)	676:725	Surface compositions derived from infrared reflection-absorption spectroscopy (IRAS) and film thickness information (atomic force microscopy, ellipsometry) suggest the latter to be statistically incorporated in the monolayer.
31117738	6	17	from	dependence	940:949	arg1	density					988:994	density	988:994	density	988:994	These surfaces' affinity for the lectin hemagglutinin revealed a strong dependence of the affinity on the presentation, density, and mobility of the sialic acid ligands.
31117738	6	17	from	dependence	940:949	arg1	mobility					1001:1008	mobility	1001:1008	mobility	1001:1008	These surfaces' affinity for the lectin hemagglutinin revealed a strong dependence of the affinity on the presentation, density, and mobility of the sialic acid ligands.
31117738	6	17	from	dependence	940:949	arg1	presentation					974:985	presentation	974:985	presentation	974:985	These surfaces' affinity for the lectin hemagglutinin revealed a strong dependence of the affinity on the presentation, density, and mobility of the sialic acid ligands.
31117738	4	18	theme	simple	516:521	arg1	immersion					523:531	simple immersion	516:531	simple immersion of a negatively charged surface in a mixture of sialic acid- and hydroxy-terminated benzamidine amphiphiles	516:639	Mixed rSAMs were prepared in water by simple immersion of a negatively charged surface in a mixture of sialic acid- and hydroxy-terminated benzamidine amphiphiles.
31117738	7	19	theme	dissociation	1125:1136	arg1	constant					1138:1145	a dissociation constant	1123:1145	a dissociation constant Kd	1123:1148	Hence, a spacer length of 4 ethylene glycol and a surface density of 15% resulted in a dissociation constant Kd,multi of 1.3 × 10-13 M, on par with the best di- or tri-saccharide-based binders reported to date, whereas a density of 20% demonstrated complete resistance to hemagglutinin binding.
31117738	5	20	theme	reflection-absorption	685:705	arg1	IRAS					721:724	IRAS	721:724	IRAS	721:724	Surface compositions derived from infrared reflection-absorption spectroscopy (IRAS) and film thickness information (atomic force microscopy, ellipsometry) suggest the latter to be statistically incorporated in the monolayer.
31117738	5	20	theme	reflection-absorption	685:705	arg1	spectroscopy					707:718	infrared reflection-absorption spectroscopy	676:718	infrared reflection-absorption spectroscopy (IRAS)	676:725	Surface compositions derived from infrared reflection-absorption spectroscopy (IRAS) and film thickness information (atomic force microscopy, ellipsometry) suggest the latter to be statistically incorporated in the monolayer.
31117738	2	21	theme	basic	333:337	arg1	studies					339:345	basic studies	333:345	basic studies of these phenomena	333:364	The lack of tuneable simplified models mimicking this dynamic environment complicates basic studies of these phenomena.
31117738	1	22	theme	multivalent	192:202	arg1	association					204:214	multivalent association	192:214	multivalent association with extracellular messengers	192:244	Glycans at the surface of cellular membranes modulate biological activity via multivalent association with extracellular messengers.
31117738	3	23	theme	mixed	395:399	arg1	rSAMs					439:443	rSAMs	439:443	rSAMs	439:443	We here present a series of mixed reversible self-assembled monolayers (rSAMs) that addresses this deficiency.
31117738	3	23	theme	mixed	395:399	arg1	monolayers					427:436	mixed reversible self-assembled monolayers	395:436	mixed reversible self-assembled monolayers (rSAMs)	395:444	We here present a series of mixed reversible self-assembled monolayers (rSAMs) that addresses this deficiency.
31117738	7	24	theme	constant	1138:1145	arg1	Kd					1147:1148	a dissociation constant Kd	1123:1148	a dissociation constant Kd	1123:1148	Hence, a spacer length of 4 ethylene glycol and a surface density of 15% resulted in a dissociation constant Kd,multi of 1.3 × 10-13 M, on par with the best di- or tri-saccharide-based binders reported to date, whereas a density of 20% demonstrated complete resistance to hemagglutinin binding.
31117738	5	25	attach	derived	663:669	arg1	IRAS					721:724	IRAS	721:724	IRAS	721:724	Surface compositions derived from infrared reflection-absorption spectroscopy (IRAS) and film thickness information (atomic force microscopy, ellipsometry) suggest the latter to be statistically incorporated in the monolayer.
31117738	5	25	attach	derived	663:669	arg1	spectroscopy					707:718	infrared reflection-absorption spectroscopy	676:718	infrared reflection-absorption spectroscopy (IRAS)	676:725	Surface compositions derived from infrared reflection-absorption spectroscopy (IRAS) and film thickness information (atomic force microscopy, ellipsometry) suggest the latter to be statistically incorporated in the monolayer.
31117738	5	25	attach	derived	663:669	arg2	compositions					650:661	Surface compositions	642:661	Surface compositions derived from infrared reflection-absorption spectroscopy (IRAS) and film thickness information (atomic force microscopy, ellipsometry)	642:796	Surface compositions derived from infrared reflection-absorption spectroscopy (IRAS) and film thickness information (atomic force microscopy, ellipsometry) suggest the latter to be statistically incorporated in the monolayer.
31117738	5	25	attach	derived	663:669	arg1	information					746:756	film thickness information	731:756	film thickness information (atomic force microscopy, ellipsometry)	731:796	Surface compositions derived from infrared reflection-absorption spectroscopy (IRAS) and film thickness information (atomic force microscopy, ellipsometry) suggest the latter to be statistically incorporated in the monolayer.
31117738	0	26	theme	Bilayer-like	6:17	arg1	Monolayers					40:49	Lipid Bilayer-like Mixed Self-Assembled Monolayers	0:49	Lipid Bilayer-like Mixed Self-Assembled Monolayers with Strong Mobility and Clustering-Dependent Lectin Affinity	0:111	Lipid Bilayer-like Mixed Self-Assembled Monolayers with Strong Mobility and Clustering-Dependent Lectin Affinity.
31117738	3	27	theme	monolayers	427:436	arg1	series					385:390	a series	383:390	a series of mixed reversible self-assembled monolayers (rSAMs) that addresses this deficiency	383:475	We here present a series of mixed reversible self-assembled monolayers (rSAMs) that addresses this deficiency.
31117738	4	28	from	immersion	523:531	arg1	mixture					570:576	a mixture	568:576	a mixture of sialic acid- and hydroxy-terminated benzamidine amphiphiles	568:639	Mixed rSAMs were prepared in water by simple immersion of a negatively charged surface in a mixture of sialic acid- and hydroxy-terminated benzamidine amphiphiles.
31117738	7	29	theme	hemagglutinin	1310:1322	arg1	binding					1324:1330	hemagglutinin binding	1310:1330	hemagglutinin binding	1310:1330	Hence, a spacer length of 4 ethylene glycol and a surface density of 15% resulted in a dissociation constant Kd,multi of 1.3 × 10-13 M, on par with the best di- or tri-saccharide-based binders reported to date, whereas a density of 20% demonstrated complete resistance to hemagglutinin binding.
31117738	0	30	theme	Lipid	0:4	arg1	Monolayers					40:49	Lipid Bilayer-like Mixed Self-Assembled Monolayers	0:49	Lipid Bilayer-like Mixed Self-Assembled Monolayers with Strong Mobility and Clustering-Dependent Lectin Affinity	0:111	Lipid Bilayer-like Mixed Self-Assembled Monolayers with Strong Mobility and Clustering-Dependent Lectin Affinity.
31117738	9	31	theme	sensors	1642:1648	arg1	design					1613:1618	the design	1609:1618	the design of model surfaces and sensors for both fundamental and applied studies	1609:1689	The results have a direct bearing on biological cell surface multivalent recognition involving lipid bilayers and may guide the design of model surfaces and sensors for both fundamental and applied studies.
31117738	1	32	from	surface	129:135	arg1	Glycans					114:120	Glycans	114:120	Glycans at the surface of cellular membranes	114:157	Glycans at the surface of cellular membranes modulate biological activity via multivalent association with extracellular messengers.
31117738	6	33	theme	acid	1024:1027	arg1	ligands					1029:1035	the sialic acid ligands	1013:1035	the sialic acid ligands	1013:1035	These surfaces' affinity for the lectin hemagglutinin revealed a strong dependence of the affinity on the presentation, density, and mobility of the sialic acid ligands.
31117738	8	34	from	drop	1458:1461	arg1	interval					1475:1482	the same interval	1466:1482	the same interval	1466:1482	These results correlated with ligand mobility measured by fluorescence recovery after photobleaching which showed a dramatic drop in the same interval.
31117738	1	35	theme	extracellular	221:233	arg1	messengers					235:244	extracellular messengers	221:244	extracellular messengers	221:244	Glycans at the surface of cellular membranes modulate biological activity via multivalent association with extracellular messengers.
31117738	0	36	theme	Self-Assembled	25:38	arg1	Monolayers					40:49	Lipid Bilayer-like Mixed Self-Assembled Monolayers	0:49	Lipid Bilayer-like Mixed Self-Assembled Monolayers with Strong Mobility and Clustering-Dependent Lectin Affinity	0:111	Lipid Bilayer-like Mixed Self-Assembled Monolayers with Strong Mobility and Clustering-Dependent Lectin Affinity.
31117738	4	37	theme	acid-	588:592	arg1	amphiphiles					629:639	sialic acid- and hydroxy-terminated benzamidine amphiphiles	581:639	sialic acid- and hydroxy-terminated benzamidine amphiphiles	581:639	Mixed rSAMs were prepared in water by simple immersion of a negatively charged surface in a mixture of sialic acid- and hydroxy-terminated benzamidine amphiphiles.
31117738	7	38	theme	%	1109:1109	arg1	length					1054:1059	a spacer length	1045:1059	a spacer length of 4 ethylene glycol	1045:1080	Hence, a spacer length of 4 ethylene glycol and a surface density of 15% resulted in a dissociation constant Kd,multi of 1.3 × 10-13 M, on par with the best di- or tri-saccharide-based binders reported to date, whereas a density of 20% demonstrated complete resistance to hemagglutinin binding.
31117738	7	38	theme	%	1109:1109	arg1	density					1096:1102	a surface density	1086:1102	a surface density of 15%	1086:1109	Hence, a spacer length of 4 ethylene glycol and a surface density of 15% resulted in a dissociation constant Kd,multi of 1.3 × 10-13 M, on par with the best di- or tri-saccharide-based binders reported to date, whereas a density of 20% demonstrated complete resistance to hemagglutinin binding.
31117738	2	39	theme	phenomena	356:364	arg1	studies					339:345	basic studies	333:345	basic studies of these phenomena	333:364	The lack of tuneable simplified models mimicking this dynamic environment complicates basic studies of these phenomena.
31117738	9	40	contain	have	1497:1500	arg1	results					1489:1495	The results	1485:1495	The results	1485:1495	The results have a direct bearing on biological cell surface multivalent recognition involving lipid bilayers and may guide the design of model surfaces and sensors for both fundamental and applied studies.
31117738	9	40	contain	have	1497:1500	arg2	bearing					1511:1517	a direct bearing	1502:1517	a direct bearing	1502:1517	The results have a direct bearing on biological cell surface multivalent recognition involving lipid bilayers and may guide the design of model surfaces and sensors for both fundamental and applied studies.
31117738	0	41	theme	Mixed	19:23	arg1	Monolayers					40:49	Lipid Bilayer-like Mixed Self-Assembled Monolayers	0:49	Lipid Bilayer-like Mixed Self-Assembled Monolayers with Strong Mobility and Clustering-Dependent Lectin Affinity	0:111	Lipid Bilayer-like Mixed Self-Assembled Monolayers with Strong Mobility and Clustering-Dependent Lectin Affinity.
31117738	4	42	theme	benzamidine	617:627	arg1	amphiphiles					629:639	sialic acid- and hydroxy-terminated benzamidine amphiphiles	581:639	sialic acid- and hydroxy-terminated benzamidine amphiphiles	581:639	Mixed rSAMs were prepared in water by simple immersion of a negatively charged surface in a mixture of sialic acid- and hydroxy-terminated benzamidine amphiphiles.
31117738	8	43	theme	ligand	1363:1368	arg1	mobility					1370:1377	ligand mobility	1363:1377	ligand mobility measured by fluorescence recovery after photobleaching which showed a dramatic drop in the same interval	1363:1482	These results correlated with ligand mobility measured by fluorescence recovery after photobleaching which showed a dramatic drop in the same interval.
31117738	9	44	theme	multivalent	1546:1556	arg1	recognition					1558:1568	biological cell surface multivalent recognition	1522:1568	biological cell surface multivalent recognition involving lipid bilayers	1522:1593	The results have a direct bearing on biological cell surface multivalent recognition involving lipid bilayers and may guide the design of model surfaces and sensors for both fundamental and applied studies.
31117738	5	45	theme	film	731:734	arg1	thickness					736:744	thickness	736:744	thickness	736:744	Surface compositions derived from infrared reflection-absorption spectroscopy (IRAS) and film thickness information (atomic force microscopy, ellipsometry) suggest the latter to be statistically incorporated in the monolayer.
31117738	9	46	theme	applied	1675:1681	arg1	studies					1683:1689	both fundamental and applied studies	1654:1689	both fundamental and applied studies	1654:1689	The results have a direct bearing on biological cell surface multivalent recognition involving lipid bilayers and may guide the design of model surfaces and sensors for both fundamental and applied studies.
31117738	4	47	theme	hydroxy-terminated	598:615	arg1	amphiphiles					629:639	sialic acid- and hydroxy-terminated benzamidine amphiphiles	581:639	sialic acid- and hydroxy-terminated benzamidine amphiphiles	581:639	Mixed rSAMs were prepared in water by simple immersion of a negatively charged surface in a mixture of sialic acid- and hydroxy-terminated benzamidine amphiphiles.
31117738	9	48	theme	direct	1504:1509	arg1	bearing					1511:1517	a direct bearing	1502:1517	a direct bearing	1502:1517	The results have a direct bearing on biological cell surface multivalent recognition involving lipid bilayers and may guide the design of model surfaces and sensors for both fundamental and applied studies.
31117738	2	49	theme	models	279:284	arg1	lack					251:254	The lack	247:254	The lack of tuneable simplified models mimicking this dynamic environment	247:319	The lack of tuneable simplified models mimicking this dynamic environment complicates basic studies of these phenomena.
31117738	7	50	theme	M	1171:1171	arg1	multi					1150:1154	multi	1150:1154	multi of 1.3 × 10-13 M	1150:1171	Hence, a spacer length of 4 ethylene glycol and a surface density of 15% resulted in a dissociation constant Kd,multi of 1.3 × 10-13 M, on par with the best di- or tri-saccharide-based binders reported to date, whereas a density of 20% demonstrated complete resistance to hemagglutinin binding.
31117738	6	51	theme	sialic	1017:1022	arg1	ligands					1029:1035	the sialic acid ligands	1013:1035	the sialic acid ligands	1013:1035	These surfaces' affinity for the lectin hemagglutinin revealed a strong dependence of the affinity on the presentation, density, and mobility of the sialic acid ligands.
31117738	2	52	theme	simplified	268:277	arg1	models					279:284	tuneable simplified models	259:284	tuneable simplified models mimicking this dynamic environment	259:319	The lack of tuneable simplified models mimicking this dynamic environment complicates basic studies of these phenomena.
31117738	7	53	theme	ethylene	1066:1073	arg1	glycol					1075:1080	4 ethylene glycol	1064:1080	4 ethylene glycol	1064:1080	Hence, a spacer length of 4 ethylene glycol and a surface density of 15% resulted in a dissociation constant Kd,multi of 1.3 × 10-13 M, on par with the best di- or tri-saccharide-based binders reported to date, whereas a density of 20% demonstrated complete resistance to hemagglutinin binding.
31117738	7	54	theme	complete	1287:1294	arg1	resistance					1296:1305	complete resistance	1287:1305	complete resistance to hemagglutinin binding	1287:1330	Hence, a spacer length of 4 ethylene glycol and a surface density of 15% resulted in a dissociation constant Kd,multi of 1.3 × 10-13 M, on par with the best di- or tri-saccharide-based binders reported to date, whereas a density of 20% demonstrated complete resistance to hemagglutinin binding.
31117738	4	55	from	mixture	570:576	arg1	immersion					523:531	simple immersion	516:531	simple immersion of a negatively charged surface in a mixture of sialic acid- and hydroxy-terminated benzamidine amphiphiles	516:639	Mixed rSAMs were prepared in water by simple immersion of a negatively charged surface in a mixture of sialic acid- and hydroxy-terminated benzamidine amphiphiles.
31117738	7	56	theme	di-	1195:1197	arg1	binders					1223:1229	the best di- or tri-saccharide-based binders	1186:1229	the best di- or tri-saccharide-based binders reported to date	1186:1246	Hence, a spacer length of 4 ethylene glycol and a surface density of 15% resulted in a dissociation constant Kd,multi of 1.3 × 10-13 M, on par with the best di- or tri-saccharide-based binders reported to date, whereas a density of 20% demonstrated complete resistance to hemagglutinin binding.
31117738	7	57	theme	surface	1088:1094	arg1	density					1096:1102	a surface density	1086:1102	a surface density of 15%	1086:1109	Hence, a spacer length of 4 ethylene glycol and a surface density of 15% resulted in a dissociation constant Kd,multi of 1.3 × 10-13 M, on par with the best di- or tri-saccharide-based binders reported to date, whereas a density of 20% demonstrated complete resistance to hemagglutinin binding.
31117738	6	58	theme	strong	933:938	arg1	dependence					940:949	a strong dependence	931:949	a strong dependence of the affinity on the presentation, density, and mobility of the sialic acid ligands	931:1035	These surfaces' affinity for the lectin hemagglutinin revealed a strong dependence of the affinity on the presentation, density, and mobility of the sialic acid ligands.
31117738	2	59	theme	tuneable	259:266	arg1	models					279:284	tuneable simplified models	259:284	tuneable simplified models mimicking this dynamic environment	259:319	The lack of tuneable simplified models mimicking this dynamic environment complicates basic studies of these phenomena.
31117738	5	60	theme	Surface	642:648	arg1	compositions					650:661	Surface compositions	642:661	Surface compositions derived from infrared reflection-absorption spectroscopy (IRAS) and film thickness information (atomic force microscopy, ellipsometry)	642:796	Surface compositions derived from infrared reflection-absorption spectroscopy (IRAS) and film thickness information (atomic force microscopy, ellipsometry) suggest the latter to be statistically incorporated in the monolayer.
31117738	5	61	dep	information	746:756	arg1	ellipsometry					784:795	ellipsometry	784:795	ellipsometry	784:795	Surface compositions derived from infrared reflection-absorption spectroscopy (IRAS) and film thickness information (atomic force microscopy, ellipsometry) suggest the latter to be statistically incorporated in the monolayer.
31117738	5	61	dep	information	746:756	arg1	microscopy					772:781	atomic force microscopy	759:781	atomic force microscopy	759:781	Surface compositions derived from infrared reflection-absorption spectroscopy (IRAS) and film thickness information (atomic force microscopy, ellipsometry) suggest the latter to be statistically incorporated in the monolayer.
31117738	0	62	theme	Strong	56:61	arg1	Mobility					63:70	Strong Mobility	56:70	Strong Mobility	56:70	Lipid Bilayer-like Mixed Self-Assembled Monolayers with Strong Mobility and Clustering-Dependent Lectin Affinity.
31117738	4	63	theme	sialic	581:586	arg1	amphiphiles					629:639	sialic acid- and hydroxy-terminated benzamidine amphiphiles	581:639	sialic acid- and hydroxy-terminated benzamidine amphiphiles	581:639	Mixed rSAMs were prepared in water by simple immersion of a negatively charged surface in a mixture of sialic acid- and hydroxy-terminated benzamidine amphiphiles.
31117738	5	64	theme	atomic	759:764	arg1	ellipsometry					784:795	ellipsometry	784:795	ellipsometry	784:795	Surface compositions derived from infrared reflection-absorption spectroscopy (IRAS) and film thickness information (atomic force microscopy, ellipsometry) suggest the latter to be statistically incorporated in the monolayer.
31117738	5	64	theme	atomic	759:764	arg1	microscopy					772:781	atomic force microscopy	759:781	atomic force microscopy	759:781	Surface compositions derived from infrared reflection-absorption spectroscopy (IRAS) and film thickness information (atomic force microscopy, ellipsometry) suggest the latter to be statistically incorporated in the monolayer.
31117738	4	65	theme	Mixed	478:482	arg1	rSAMs					484:488	Mixed rSAMs	478:488	Mixed rSAMs	478:488	Mixed rSAMs were prepared in water by simple immersion of a negatively charged surface in a mixture of sialic acid- and hydroxy-terminated benzamidine amphiphiles.
31117738	9	66	theme	surfaces	1629:1636	arg1	design					1613:1618	the design	1609:1618	the design of model surfaces and sensors for both fundamental and applied studies	1609:1689	The results have a direct bearing on biological cell surface multivalent recognition involving lipid bilayers and may guide the design of model surfaces and sensors for both fundamental and applied studies.
31117738	6	67	theme	affinity	958:965	arg1	dependence					940:949	a strong dependence	931:949	a strong dependence of the affinity on the presentation, density, and mobility of the sialic acid ligands	931:1035	These surfaces' affinity for the lectin hemagglutinin revealed a strong dependence of the affinity on the presentation, density, and mobility of the sialic acid ligands.
31117738	8	68	theme	dramatic	1449:1456	arg1	drop					1458:1461	a dramatic drop	1447:1461	a dramatic drop in the same interval	1447:1482	These results correlated with ligand mobility measured by fluorescence recovery after photobleaching which showed a dramatic drop in the same interval.
31117738	5	69	theme	force	766:770	arg1	ellipsometry					784:795	ellipsometry	784:795	ellipsometry	784:795	Surface compositions derived from infrared reflection-absorption spectroscopy (IRAS) and film thickness information (atomic force microscopy, ellipsometry) suggest the latter to be statistically incorporated in the monolayer.
31117738	5	69	theme	force	766:770	arg1	microscopy					772:781	atomic force microscopy	759:781	atomic force microscopy	759:781	Surface compositions derived from infrared reflection-absorption spectroscopy (IRAS) and film thickness information (atomic force microscopy, ellipsometry) suggest the latter to be statistically incorporated in the monolayer.
31117738	9	70	theme	cell	1533:1536	arg1	recognition					1558:1568	biological cell surface multivalent recognition	1522:1568	biological cell surface multivalent recognition involving lipid bilayers	1522:1593	The results have a direct bearing on biological cell surface multivalent recognition involving lipid bilayers and may guide the design of model surfaces and sensors for both fundamental and applied studies.
31117738	1	71	theme	cellular	140:147	arg1	membranes					149:157	cellular membranes	140:157	cellular membranes	140:157	Glycans at the surface of cellular membranes modulate biological activity via multivalent association with extracellular messengers.
31117738	7	72	with	par	1177:1179	arg1	binders					1223:1229	the best di- or tri-saccharide-based binders	1186:1229	the best di- or tri-saccharide-based binders reported to date	1186:1246	Hence, a spacer length of 4 ethylene glycol and a surface density of 15% resulted in a dissociation constant Kd,multi of 1.3 × 10-13 M, on par with the best di- or tri-saccharide-based binders reported to date, whereas a density of 20% demonstrated complete resistance to hemagglutinin binding.
31117738	1	73	with	association	204:214	arg1	messengers					235:244	extracellular messengers	221:244	extracellular messengers	221:244	Glycans at the surface of cellular membranes modulate biological activity via multivalent association with extracellular messengers.
31117738	2	74	theme	dynamic	301:307	arg1	environment					309:319	this dynamic environment	296:319	this dynamic environment	296:319	The lack of tuneable simplified models mimicking this dynamic environment complicates basic studies of these phenomena.
31117738	7	75	theme	spacer	1047:1052	arg1	length					1054:1059	a spacer length	1045:1059	a spacer length of 4 ethylene glycol	1045:1080	Hence, a spacer length of 4 ethylene glycol and a surface density of 15% resulted in a dissociation constant Kd,multi of 1.3 × 10-13 M, on par with the best di- or tri-saccharide-based binders reported to date, whereas a density of 20% demonstrated complete resistance to hemagglutinin binding.
31117738	1	76	theme	membranes	149:157	arg1	surface					129:135	the surface	125:135	the surface of cellular membranes	125:157	Glycans at the surface of cellular membranes modulate biological activity via multivalent association with extracellular messengers.
31117738	0	77	theme	Clustering-Dependent	76:95	arg1	Affinity					104:111	Clustering-Dependent Lectin Affinity	76:111	Clustering-Dependent Lectin Affinity	76:111	Lipid Bilayer-like Mixed Self-Assembled Monolayers with Strong Mobility and Clustering-Dependent Lectin Affinity.
31117738	6	78	theme	lectin	901:906	arg1	hemagglutinin					908:920	the lectin hemagglutinin	897:920	the lectin hemagglutinin	897:920	These surfaces' affinity for the lectin hemagglutinin revealed a strong dependence of the affinity on the presentation, density, and mobility of the sialic acid ligands.
31117738	9	79	theme	biological	1522:1531	arg1	recognition					1558:1568	biological cell surface multivalent recognition	1522:1568	biological cell surface multivalent recognition involving lipid bilayers	1522:1593	The results have a direct bearing on biological cell surface multivalent recognition involving lipid bilayers and may guide the design of model surfaces and sensors for both fundamental and applied studies.
31117738	8	80	theme	fluorescence	1391:1402	arg1	recovery					1404:1411	fluorescence recovery	1391:1411	fluorescence recovery	1391:1411	These results correlated with ligand mobility measured by fluorescence recovery after photobleaching which showed a dramatic drop in the same interval.
31117738	6	81	dep	presentation	974:985	arg1	the					970:972	the	970:972	the	970:972	These surfaces' affinity for the lectin hemagglutinin revealed a strong dependence of the affinity on the presentation, density, and mobility of the sialic acid ligands.
31117738	4	82	theme	charged	549:555	arg1	surface					557:563	a negatively charged surface	536:563	a negatively charged surface in a mixture of sialic acid- and hydroxy-terminated benzamidine amphiphiles	536:639	Mixed rSAMs were prepared in water by simple immersion of a negatively charged surface in a mixture of sialic acid- and hydroxy-terminated benzamidine amphiphiles.
31117738	7	83	theme	%	1272:1272	arg1	density					1259:1265	a density	1257:1265	a density of 20%	1257:1272	Hence, a spacer length of 4 ethylene glycol and a surface density of 15% resulted in a dissociation constant Kd,multi of 1.3 × 10-13 M, on par with the best di- or tri-saccharide-based binders reported to date, whereas a density of 20% demonstrated complete resistance to hemagglutinin binding.
31117738	1	84	theme	biological	168:177	arg1	activity					179:186	biological activity	168:186	biological activity	168:186	Glycans at the surface of cellular membranes modulate biological activity via multivalent association with extracellular messengers.
29725011	4	0	theme	functional	715:724	arg1	pathways					736:743	functional metabolic pathways	715:743	functional metabolic pathways	715:743	Metagenomic sequencing of gorilla microbiomes demonstrates distinctions in functional metabolic pathways, archaea, and dietary plants among enterotypes, suggesting that dietary seasonality dictates shifts in the microbiome and its capacity for microbial plant fiber digestion versus growth on mucus glycans.
29725011	1	1	from	diets	161:165	arg1	extraction					109:118	extraction	109:118	extraction of energy and nutrition from plant-based diets	109:165	The microbiome is essential for extraction of energy and nutrition from plant-based diets and may have facilitated primate adaptation to new dietary niches in response to rapid environmental shifts.
29725011	1	2	theme	nutrition	134:142	arg1	extraction					109:118	extraction	109:118	extraction of energy and nutrition from plant-based diets	109:165	The microbiome is essential for extraction of energy and nutrition from plant-based diets and may have facilitated primate adaptation to new dietary niches in response to rapid environmental shifts.
29725011	5	3	theme	microbiomes	1227:1237	arg1	evolution					1204:1212	the evolution	1200:1212	the evolution of human gut microbiomes	1200:1237	These data indicate that great ape microbiomes are malleable in response to dietary shifts, suggesting a role for microbiome plasticity in driving dietary flexibility, which may provide fundamental insights into the mechanisms by which diet has driven the evolution of human gut microbiomes.
29725011	2	4	theme	chimpanzees	484:494	arg1	microbiotas					459:469	the microbiotas	455:469	the microbiotas of gorillas, chimpanzees, Old World monkeys, and modern humans	455:532	Here we use 16S rRNA sequencing to characterize the microbiota of wild western lowland gorillas and sympatric central chimpanzees and demonstrate compositional divergence between the microbiotas of gorillas, chimpanzees, Old World monkeys, and modern humans.
29725011	4	5	theme	fiber	900:904	arg1	digestion					906:914	microbial plant fiber digestion	884:914	microbial plant fiber digestion	884:914	Metagenomic sequencing of gorilla microbiomes demonstrates distinctions in functional metabolic pathways, archaea, and dietary plants among enterotypes, suggesting that dietary seasonality dictates shifts in the microbiome and its capacity for microbial plant fiber digestion versus growth on mucus glycans.
29725011	4	6	theme	metabolic	726:734	arg1	pathways					736:743	functional metabolic pathways	715:743	functional metabolic pathways	715:743	Metagenomic sequencing of gorilla microbiomes demonstrates distinctions in functional metabolic pathways, archaea, and dietary plants among enterotypes, suggesting that dietary seasonality dictates shifts in the microbiome and its capacity for microbial plant fiber digestion versus growth on mucus glycans.
29725011	5	7	from	malleable	999:1007	arg1	response					1012:1019	response	1012:1019	response to dietary shifts	1012:1037	These data indicate that great ape microbiomes are malleable in response to dietary shifts, suggesting a role for microbiome plasticity in driving dietary flexibility, which may provide fundamental insights into the mechanisms by which diet has driven the evolution of human gut microbiomes.
29725011	2	8	theme	modern	520:525	arg1	humans					527:532	modern humans	520:532	modern humans	520:532	Here we use 16S rRNA sequencing to characterize the microbiota of wild western lowland gorillas and sympatric central chimpanzees and demonstrate compositional divergence between the microbiotas of gorillas, chimpanzees, Old World monkeys, and modern humans.
29725011	3	9	theme	chimpanzee	560:569	arg1	microbiomes					571:581	gorilla and chimpanzee microbiomes	548:581	gorilla and chimpanzee microbiomes	548:581	We show that gorilla and chimpanzee microbiomes fluctuate with seasonal rainfall patterns and frugivory.
29725011	4	10	theme	microbiomes	674:684	arg1	sequencing					652:661	Metagenomic sequencing	640:661	Metagenomic sequencing of gorilla microbiomes	640:684	Metagenomic sequencing of gorilla microbiomes demonstrates distinctions in functional metabolic pathways, archaea, and dietary plants among enterotypes, suggesting that dietary seasonality dictates shifts in the microbiome and its capacity for microbial plant fiber digestion versus growth on mucus glycans.
29725011	2	11	theme	rRNA	292:295	arg1	sequencing					297:306	16S rRNA sequencing	288:306	16S rRNA sequencing	288:306	Here we use 16S rRNA sequencing to characterize the microbiota of wild western lowland gorillas and sympatric central chimpanzees and demonstrate compositional divergence between the microbiotas of gorillas, chimpanzees, Old World monkeys, and modern humans.
29725011	4	12	from	shifts	838:843	arg1	glycans					939:945	mucus glycans	933:945	mucus glycans	933:945	Metagenomic sequencing of gorilla microbiomes demonstrates distinctions in functional metabolic pathways, archaea, and dietary plants among enterotypes, suggesting that dietary seasonality dictates shifts in the microbiome and its capacity for microbial plant fiber digestion versus growth on mucus glycans.
29725011	4	12	from	shifts	838:843	arg1	microbiome					852:861	microbiome	852:861	microbiome	852:861	Metagenomic sequencing of gorilla microbiomes demonstrates distinctions in functional metabolic pathways, archaea, and dietary plants among enterotypes, suggesting that dietary seasonality dictates shifts in the microbiome and its capacity for microbial plant fiber digestion versus growth on mucus glycans.
29725011	4	12	from	shifts	838:843	arg1	capacity					871:878	its capacity	867:878	its capacity	867:878	Metagenomic sequencing of gorilla microbiomes demonstrates distinctions in functional metabolic pathways, archaea, and dietary plants among enterotypes, suggesting that dietary seasonality dictates shifts in the microbiome and its capacity for microbial plant fiber digestion versus growth on mucus glycans.
29725011	4	12	from	shifts	838:843	arg1	growth					923:928	growth	923:928	growth on mucus glycans	923:945	Metagenomic sequencing of gorilla microbiomes demonstrates distinctions in functional metabolic pathways, archaea, and dietary plants among enterotypes, suggesting that dietary seasonality dictates shifts in the microbiome and its capacity for microbial plant fiber digestion versus growth on mucus glycans.
29725011	4	13	from	distinctions	699:710	arg1	archaea					746:752	archaea	746:752	archaea	746:752	Metagenomic sequencing of gorilla microbiomes demonstrates distinctions in functional metabolic pathways, archaea, and dietary plants among enterotypes, suggesting that dietary seasonality dictates shifts in the microbiome and its capacity for microbial plant fiber digestion versus growth on mucus glycans.
29725011	4	13	from	distinctions	699:710	arg1	pathways					736:743	functional metabolic pathways	715:743	functional metabolic pathways	715:743	Metagenomic sequencing of gorilla microbiomes demonstrates distinctions in functional metabolic pathways, archaea, and dietary plants among enterotypes, suggesting that dietary seasonality dictates shifts in the microbiome and its capacity for microbial plant fiber digestion versus growth on mucus glycans.
29725011	4	13	from	distinctions	699:710	arg1	plants					767:772	dietary plants	759:772	dietary plants among enterotypes	759:790	Metagenomic sequencing of gorilla microbiomes demonstrates distinctions in functional metabolic pathways, archaea, and dietary plants among enterotypes, suggesting that dietary seasonality dictates shifts in the microbiome and its capacity for microbial plant fiber digestion versus growth on mucus glycans.
29725011	1	14	theme	rapid	248:252	arg1	shifts					268:273	rapid environmental shifts	248:273	rapid environmental shifts	248:273	The microbiome is essential for extraction of energy and nutrition from plant-based diets and may have facilitated primate adaptation to new dietary niches in response to rapid environmental shifts.
29725011	5	15	theme	fundamental	1134:1144	arg1	insights					1146:1153	fundamental insights	1134:1153	fundamental insights into the mechanisms by which diet has driven the evolution of human gut microbiomes	1134:1237	These data indicate that great ape microbiomes are malleable in response to dietary shifts, suggesting a role for microbiome plasticity in driving dietary flexibility, which may provide fundamental insights into the mechanisms by which diet has driven the evolution of human gut microbiomes.
29725011	4	16	theme	dietary	809:815	arg1	seasonality					817:827	dietary seasonality	809:827	dietary seasonality	809:827	Metagenomic sequencing of gorilla microbiomes demonstrates distinctions in functional metabolic pathways, archaea, and dietary plants among enterotypes, suggesting that dietary seasonality dictates shifts in the microbiome and its capacity for microbial plant fiber digestion versus growth on mucus glycans.
29725011	1	17	theme	plant-based	149:159	arg1	diets					161:165	plant-based diets	149:165	plant-based diets	149:165	The microbiome is essential for extraction of energy and nutrition from plant-based diets and may have facilitated primate adaptation to new dietary niches in response to rapid environmental shifts.
29725011	2	18	theme	monkeys	507:513	arg1	microbiotas					459:469	the microbiotas	455:469	the microbiotas of gorillas, chimpanzees, Old World monkeys, and modern humans	455:532	Here we use 16S rRNA sequencing to characterize the microbiota of wild western lowland gorillas and sympatric central chimpanzees and demonstrate compositional divergence between the microbiotas of gorillas, chimpanzees, Old World monkeys, and modern humans.
29725011	5	19	theme	great	973:977	arg1	microbiomes					983:993	great ape microbiomes	973:993	great ape microbiomes	973:993	These data indicate that great ape microbiomes are malleable in response to dietary shifts, suggesting a role for microbiome plasticity in driving dietary flexibility, which may provide fundamental insights into the mechanisms by which diet has driven the evolution of human gut microbiomes.
29725011	4	20	theme	gorilla	666:672	arg1	microbiomes					674:684	gorilla microbiomes	666:684	gorilla microbiomes	666:684	Metagenomic sequencing of gorilla microbiomes demonstrates distinctions in functional metabolic pathways, archaea, and dietary plants among enterotypes, suggesting that dietary seasonality dictates shifts in the microbiome and its capacity for microbial plant fiber digestion versus growth on mucus glycans.
29725011	2	21	theme	gorillas	474:481	arg1	microbiotas					459:469	the microbiotas	455:469	the microbiotas of gorillas, chimpanzees, Old World monkeys, and modern humans	455:532	Here we use 16S rRNA sequencing to characterize the microbiota of wild western lowland gorillas and sympatric central chimpanzees and demonstrate compositional divergence between the microbiotas of gorillas, chimpanzees, Old World monkeys, and modern humans.
29725011	4	22	from	capacity	871:878	arg1	glycans					939:945	mucus glycans	933:945	mucus glycans	933:945	Metagenomic sequencing of gorilla microbiomes demonstrates distinctions in functional metabolic pathways, archaea, and dietary plants among enterotypes, suggesting that dietary seasonality dictates shifts in the microbiome and its capacity for microbial plant fiber digestion versus growth on mucus glycans.
29725011	4	23	theme	plant	894:898	arg1	digestion					906:914	microbial plant fiber digestion	884:914	microbial plant fiber digestion	884:914	Metagenomic sequencing of gorilla microbiomes demonstrates distinctions in functional metabolic pathways, archaea, and dietary plants among enterotypes, suggesting that dietary seasonality dictates shifts in the microbiome and its capacity for microbial plant fiber digestion versus growth on mucus glycans.
29725011	0	24	dep	diet	71:74	arg1	response					59:66	response	59:66	response	59:66	Gut microbiomes of wild great apes fluctuate seasonally in response to diet.
29725011	2	25	theme	gorillas	363:370	arg1	microbiota					328:337	the microbiota	324:337	the microbiota of wild western lowland gorillas and sympatric central chimpanzees	324:404	Here we use 16S rRNA sequencing to characterize the microbiota of wild western lowland gorillas and sympatric central chimpanzees and demonstrate compositional divergence between the microbiotas of gorillas, chimpanzees, Old World monkeys, and modern humans.
29725011	0	26	theme	Gut	0:2	arg1	microbiomes					4:14	Gut microbiomes	0:14	Gut microbiomes of wild great apes	0:33	Gut microbiomes of wild great apes fluctuate seasonally in response to diet.
29725011	1	27	theme	environmental	254:266	arg1	shifts					268:273	rapid environmental shifts	248:273	rapid environmental shifts	248:273	The microbiome is essential for extraction of energy and nutrition from plant-based diets and may have facilitated primate adaptation to new dietary niches in response to rapid environmental shifts.
29725011	4	28	theme	microbial	884:892	arg1	digestion					906:914	microbial plant fiber digestion	884:914	microbial plant fiber digestion	884:914	Metagenomic sequencing of gorilla microbiomes demonstrates distinctions in functional metabolic pathways, archaea, and dietary plants among enterotypes, suggesting that dietary seasonality dictates shifts in the microbiome and its capacity for microbial plant fiber digestion versus growth on mucus glycans.
29725011	2	29	theme	16S	288:290	arg1	sequencing					297:306	16S rRNA sequencing	288:306	16S rRNA sequencing	288:306	Here we use 16S rRNA sequencing to characterize the microbiota of wild western lowland gorillas and sympatric central chimpanzees and demonstrate compositional divergence between the microbiotas of gorillas, chimpanzees, Old World monkeys, and modern humans.
29725011	2	30	theme	lowland	355:361	arg1	gorillas					363:370	wild western lowland gorillas	342:370	wild western lowland gorillas	342:370	Here we use 16S rRNA sequencing to characterize the microbiota of wild western lowland gorillas and sympatric central chimpanzees and demonstrate compositional divergence between the microbiotas of gorillas, chimpanzees, Old World monkeys, and modern humans.
29725011	0	31	theme	wild	19:22	arg1	apes					30:33	wild great apes	19:33	wild great apes	19:33	Gut microbiomes of wild great apes fluctuate seasonally in response to diet.
29725011	4	32	theme	dietary	759:765	arg1	plants					767:772	dietary plants	759:772	dietary plants among enterotypes	759:790	Metagenomic sequencing of gorilla microbiomes demonstrates distinctions in functional metabolic pathways, archaea, and dietary plants among enterotypes, suggesting that dietary seasonality dictates shifts in the microbiome and its capacity for microbial plant fiber digestion versus growth on mucus glycans.
29725011	3	33	dep	rainfall	607:614	arg1	patterns					616:623	patterns	616:623	patterns	616:623	We show that gorilla and chimpanzee microbiomes fluctuate with seasonal rainfall patterns and frugivory.
29725011	3	33	dep	rainfall	607:614	arg1	rainfall					607:614	seasonal rainfall patterns and frugivory	598:637	seasonal rainfall patterns and frugivory	598:637	We show that gorilla and chimpanzee microbiomes fluctuate with seasonal rainfall patterns and frugivory.
29725011	3	33	dep	rainfall	607:614	arg1	frugivory					629:637	frugivory	629:637	frugivory	629:637	We show that gorilla and chimpanzee microbiomes fluctuate with seasonal rainfall patterns and frugivory.
29725011	2	34	theme	compositional	422:434	arg1	divergence					436:445	compositional divergence	422:445	compositional divergence between the microbiotas of gorillas, chimpanzees, Old World monkeys, and modern humans	422:532	Here we use 16S rRNA sequencing to characterize the microbiota of wild western lowland gorillas and sympatric central chimpanzees and demonstrate compositional divergence between the microbiotas of gorillas, chimpanzees, Old World monkeys, and modern humans.
29725011	5	35	theme	human	1217:1221	arg1	microbiomes					1227:1237	human gut microbiomes	1217:1237	human gut microbiomes	1217:1237	These data indicate that great ape microbiomes are malleable in response to dietary shifts, suggesting a role for microbiome plasticity in driving dietary flexibility, which may provide fundamental insights into the mechanisms by which diet has driven the evolution of human gut microbiomes.
29725011	5	36	theme	ape	979:981	arg1	microbiomes					983:993	great ape microbiomes	973:993	great ape microbiomes	973:993	These data indicate that great ape microbiomes are malleable in response to dietary shifts, suggesting a role for microbiome plasticity in driving dietary flexibility, which may provide fundamental insights into the mechanisms by which diet has driven the evolution of human gut microbiomes.
29725011	3	37	theme	gorilla	548:554	arg1	microbiomes					571:581	gorilla and chimpanzee microbiomes	548:581	gorilla and chimpanzee microbiomes	548:581	We show that gorilla and chimpanzee microbiomes fluctuate with seasonal rainfall patterns and frugivory.
29725011	1	38	theme	energy	123:128	arg1	extraction					109:118	extraction	109:118	extraction of energy and nutrition from plant-based diets	109:165	The microbiome is essential for extraction of energy and nutrition from plant-based diets and may have facilitated primate adaptation to new dietary niches in response to rapid environmental shifts.
29725011	0	39	theme	apes	30:33	arg1	microbiomes					4:14	Gut microbiomes	0:14	Gut microbiomes of wild great apes	0:33	Gut microbiomes of wild great apes fluctuate seasonally in response to diet.
29725011	5	40	theme	gut	1223:1225	arg1	microbiomes					1227:1237	human gut microbiomes	1217:1237	human gut microbiomes	1217:1237	These data indicate that great ape microbiomes are malleable in response to dietary shifts, suggesting a role for microbiome plasticity in driving dietary flexibility, which may provide fundamental insights into the mechanisms by which diet has driven the evolution of human gut microbiomes.
29725011	2	41	theme	chimpanzees	394:404	arg1	microbiota					328:337	the microbiota	324:337	the microbiota of wild western lowland gorillas and sympatric central chimpanzees	324:404	Here we use 16S rRNA sequencing to characterize the microbiota of wild western lowland gorillas and sympatric central chimpanzees and demonstrate compositional divergence between the microbiotas of gorillas, chimpanzees, Old World monkeys, and modern humans.
29725011	1	42	theme	primate	192:198	arg1	adaptation					200:209	primate adaptation	192:209	primate adaptation to new dietary niches in response to rapid environmental shifts	192:273	The microbiome is essential for extraction of energy and nutrition from plant-based diets and may have facilitated primate adaptation to new dietary niches in response to rapid environmental shifts.
29725011	0	43	theme	great	24:28	arg1	apes					30:33	wild great apes	19:33	wild great apes	19:33	Gut microbiomes of wild great apes fluctuate seasonally in response to diet.
29725011	2	44	theme	humans	527:532	arg1	microbiotas					459:469	the microbiotas	455:469	the microbiotas of gorillas, chimpanzees, Old World monkeys, and modern humans	455:532	Here we use 16S rRNA sequencing to characterize the microbiota of wild western lowland gorillas and sympatric central chimpanzees and demonstrate compositional divergence between the microbiotas of gorillas, chimpanzees, Old World monkeys, and modern humans.
29725011	3	45	theme	seasonal	598:605	arg1	patterns					616:623	patterns	616:623	patterns	616:623	We show that gorilla and chimpanzee microbiomes fluctuate with seasonal rainfall patterns and frugivory.
29725011	3	45	theme	seasonal	598:605	arg1	rainfall					607:614	seasonal rainfall patterns and frugivory	598:637	seasonal rainfall patterns and frugivory	598:637	We show that gorilla and chimpanzee microbiomes fluctuate with seasonal rainfall patterns and frugivory.
29725011	3	45	theme	seasonal	598:605	arg1	frugivory					629:637	frugivory	629:637	frugivory	629:637	We show that gorilla and chimpanzee microbiomes fluctuate with seasonal rainfall patterns and frugivory.
29725011	2	46	theme	central	386:392	arg1	chimpanzees					394:404	sympatric central chimpanzees	376:404	sympatric central chimpanzees	376:404	Here we use 16S rRNA sequencing to characterize the microbiota of wild western lowland gorillas and sympatric central chimpanzees and demonstrate compositional divergence between the microbiotas of gorillas, chimpanzees, Old World monkeys, and modern humans.
29725011	4	47	theme	mucus	933:937	arg1	glycans					939:945	mucus glycans	933:945	mucus glycans	933:945	Metagenomic sequencing of gorilla microbiomes demonstrates distinctions in functional metabolic pathways, archaea, and dietary plants among enterotypes, suggesting that dietary seasonality dictates shifts in the microbiome and its capacity for microbial plant fiber digestion versus growth on mucus glycans.
29725011	1	48	from	adaptation	200:209	arg1	response					236:243	response	236:243	response to rapid environmental shifts	236:273	The microbiome is essential for extraction of energy and nutrition from plant-based diets and may have facilitated primate adaptation to new dietary niches in response to rapid environmental shifts.
29725011	2	49	theme	sympatric	376:384	arg1	chimpanzees					394:404	sympatric central chimpanzees	376:404	sympatric central chimpanzees	376:404	Here we use 16S rRNA sequencing to characterize the microbiota of wild western lowland gorillas and sympatric central chimpanzees and demonstrate compositional divergence between the microbiotas of gorillas, chimpanzees, Old World monkeys, and modern humans.
29725011	5	50	theme	microbiome	1062:1071	arg1	plasticity					1073:1082	microbiome plasticity	1062:1082	microbiome plasticity	1062:1082	These data indicate that great ape microbiomes are malleable in response to dietary shifts, suggesting a role for microbiome plasticity in driving dietary flexibility, which may provide fundamental insights into the mechanisms by which diet has driven the evolution of human gut microbiomes.
29725011	4	51	from	microbiome	852:861	arg1	glycans					939:945	mucus glycans	933:945	mucus glycans	933:945	Metagenomic sequencing of gorilla microbiomes demonstrates distinctions in functional metabolic pathways, archaea, and dietary plants among enterotypes, suggesting that dietary seasonality dictates shifts in the microbiome and its capacity for microbial plant fiber digestion versus growth on mucus glycans.
29725011	4	52	from	glycans	939:945	arg1	shifts					838:843	shifts	838:843	shifts in the microbiome and its capacity for microbial plant fiber digestion versus growth on mucus glycans	838:945	Metagenomic sequencing of gorilla microbiomes demonstrates distinctions in functional metabolic pathways, archaea, and dietary plants among enterotypes, suggesting that dietary seasonality dictates shifts in the microbiome and its capacity for microbial plant fiber digestion versus growth on mucus glycans.
29725011	5	53	theme	dietary	1024:1030	arg1	shifts					1032:1037	dietary shifts	1024:1037	dietary shifts	1024:1037	These data indicate that great ape microbiomes are malleable in response to dietary shifts, suggesting a role for microbiome plasticity in driving dietary flexibility, which may provide fundamental insights into the mechanisms by which diet has driven the evolution of human gut microbiomes.
29725011	4	54	dep	microbiome	852:861	arg1	the					848:850	the	848:850	the	848:850	Metagenomic sequencing of gorilla microbiomes demonstrates distinctions in functional metabolic pathways, archaea, and dietary plants among enterotypes, suggesting that dietary seasonality dictates shifts in the microbiome and its capacity for microbial plant fiber digestion versus growth on mucus glycans.
29725011	1	55	theme	new	214:216	arg1	niches					226:231	new dietary niches	214:231	new dietary niches	214:231	The microbiome is essential for extraction of energy and nutrition from plant-based diets and may have facilitated primate adaptation to new dietary niches in response to rapid environmental shifts.
29725011	2	56	theme	World	501:505	arg1	monkeys					507:513	Old World monkeys	497:513	Old World monkeys	497:513	Here we use 16S rRNA sequencing to characterize the microbiota of wild western lowland gorillas and sympatric central chimpanzees and demonstrate compositional divergence between the microbiotas of gorillas, chimpanzees, Old World monkeys, and modern humans.
29725011	5	57	theme	dietary	1095:1101	arg1	flexibility					1103:1113	dietary flexibility	1095:1113	dietary flexibility	1095:1113	These data indicate that great ape microbiomes are malleable in response to dietary shifts, suggesting a role for microbiome plasticity in driving dietary flexibility, which may provide fundamental insights into the mechanisms by which diet has driven the evolution of human gut microbiomes.
29725011	4	58	theme	Metagenomic	640:650	arg1	sequencing					652:661	Metagenomic sequencing	640:661	Metagenomic sequencing of gorilla microbiomes	640:684	Metagenomic sequencing of gorilla microbiomes demonstrates distinctions in functional metabolic pathways, archaea, and dietary plants among enterotypes, suggesting that dietary seasonality dictates shifts in the microbiome and its capacity for microbial plant fiber digestion versus growth on mucus glycans.
29725011	2	59	theme	western	347:353	arg1	gorillas					363:370	wild western lowland gorillas	342:370	wild western lowland gorillas	342:370	Here we use 16S rRNA sequencing to characterize the microbiota of wild western lowland gorillas and sympatric central chimpanzees and demonstrate compositional divergence between the microbiotas of gorillas, chimpanzees, Old World monkeys, and modern humans.
29725011	1	60	theme	dietary	218:224	arg1	niches					226:231	new dietary niches	214:231	new dietary niches	214:231	The microbiome is essential for extraction of energy and nutrition from plant-based diets and may have facilitated primate adaptation to new dietary niches in response to rapid environmental shifts.
29725011	2	61	theme	Old	497:499	arg1	monkeys					507:513	Old World monkeys	497:513	Old World monkeys	497:513	Here we use 16S rRNA sequencing to characterize the microbiota of wild western lowland gorillas and sympatric central chimpanzees and demonstrate compositional divergence between the microbiotas of gorillas, chimpanzees, Old World monkeys, and modern humans.
29725011	4	62	from	growth	923:928	arg1	glycans					939:945	mucus glycans	933:945	mucus glycans	933:945	Metagenomic sequencing of gorilla microbiomes demonstrates distinctions in functional metabolic pathways, archaea, and dietary plants among enterotypes, suggesting that dietary seasonality dictates shifts in the microbiome and its capacity for microbial plant fiber digestion versus growth on mucus glycans.
29725011	2	63	theme	wild	342:345	arg1	gorillas					363:370	wild western lowland gorillas	342:370	wild western lowland gorillas	342:370	Here we use 16S rRNA sequencing to characterize the microbiota of wild western lowland gorillas and sympatric central chimpanzees and demonstrate compositional divergence between the microbiotas of gorillas, chimpanzees, Old World monkeys, and modern humans.
29725011	5	64	from	response	1012:1019	arg1	malleable					999:1007	malleable	999:1007	malleable	999:1007	These data indicate that great ape microbiomes are malleable in response to dietary shifts, suggesting a role for microbiome plasticity in driving dietary flexibility, which may provide fundamental insights into the mechanisms by which diet has driven the evolution of human gut microbiomes.
31407369	8	0	theme	spherical	1028:1036	arg1	enzyme					968:973	The enzyme	964:973	The enzyme	964:973	The enzyme had antioxidant activities and were characterized as spherical micro- and nanoparticles by transmission electron microscopy.
31407369	8	0	theme	spherical	1028:1036	arg1	micro-					1038:1043	spherical micro-	1028:1043	spherical micro-	1028:1043	The enzyme had antioxidant activities and were characterized as spherical micro- and nanoparticles by transmission electron microscopy.
31407369	9	1	theme	fibrinolytic	1182:1193	arg1	activity					1195:1202	its fibrinolytic activity	1178:1202	its fibrinolytic activity	1178:1202	The effect of growth conditions and medium composition in levan structure and its fibrinolytic activity was evaluated.
31407369	1	2	theme	isolates	142:149	arg1	Screening					110:118	Screening	110:118	Screening of 18 bacterial honey isolates	110:149	Screening of 18 bacterial honey isolates revealed that all the isolates were levansucrase producers.
31407369	8	3	theme	antioxidant	979:989	arg1	activities					991:1000	antioxidant activities	979:1000	antioxidant activities	979:1000	The enzyme had antioxidant activities and were characterized as spherical micro- and nanoparticles by transmission electron microscopy.
31407369	4	4	theme	Bacillus	516:523	arg1	levansucrase					534:545	The Bacillus subtilis levansucrase	512:545	The Bacillus subtilis levansucrase	512:545	The Bacillus subtilis levansucrase was eluted as a single protein in one purification step.
31407369	4	4	theme	Bacillus	516:523	arg1	protein					570:576	a single protein	561:576	a single protein in one purification step	561:601	The Bacillus subtilis levansucrase was eluted as a single protein in one purification step.
31407369	0	5	theme	NRC	75:77	arg1	levansucrase					82:93	Bacillus subtilis NRC 16 levansucrase	57:93	a new efficient low molecular weight Bacillus subtilis NRC 16 levansucrase	20:93	Characterization of a new efficient low molecular weight Bacillus subtilis NRC 16 levansucrase and its levan.
31407369	2	6	theme	morphological	332:344	arg1	examination					346:356	morphological examination	332:356	morphological examination	332:356	The most potent isolate that achieved the highest activity (45.66 U/ml) was identified as Bacillus subtilis NRC based on morphological examination and 16S rRNA.
31407369	6	7	theme	activity	713:720	arg1	%					704:704	60%	702:704	60% of its activity	702:720	It showed its optimum activity at 45°C and could retain 60% of its activity after incubation at 50°C for 2 h. Its optimum activity was obtained at pH 8.2 and the enzyme showed great pH stability in both acidic and alkaline ranges.
31407369	6	7	theme	activity	713:720	arg1	activity					713:720	its activity	709:720	its activity	709:720	It showed its optimum activity at 45°C and could retain 60% of its activity after incubation at 50°C for 2 h. Its optimum activity was obtained at pH 8.2 and the enzyme showed great pH stability in both acidic and alkaline ranges.
31407369	3	8	theme	enzyme	491:496	arg1	productivity					498:509	the enzyme productivity	487:509	the enzyme productivity	487:509	The results recorded the necessity of starch (5 g/L), baker's yeast (12.5 g/L), and AlCl3 (5 mM) in improvement of the enzyme productivity.
31407369	3	9	theme	productivity	498:509	arg1	improvement					472:482	improvement	472:482	improvement of the enzyme productivity	472:509	The results recorded the necessity of starch (5 g/L), baker's yeast (12.5 g/L), and AlCl3 (5 mM) in improvement of the enzyme productivity.
31407369	6	10	theme	alkaline	860:867	arg1	ranges					869:874	alkaline ranges	860:874	alkaline ranges	860:874	It showed its optimum activity at 45°C and could retain 60% of its activity after incubation at 50°C for 2 h. Its optimum activity was obtained at pH 8.2 and the enzyme showed great pH stability in both acidic and alkaline ranges.
31407369	0	11	theme	levan	103:107	arg1	Characterization					0:15	Characterization	0:15	Characterization of a new efficient low molecular weight Bacillus subtilis NRC 16 levansucrase and its levan.	0:108	Characterization of a new efficient low molecular weight Bacillus subtilis NRC 16 levansucrase and its levan.
31407369	4	12	theme	single	563:568	arg1	levansucrase					534:545	The Bacillus subtilis levansucrase	512:545	The Bacillus subtilis levansucrase	512:545	The Bacillus subtilis levansucrase was eluted as a single protein in one purification step.
31407369	4	12	theme	single	563:568	arg1	protein					570:576	a single protein	561:576	a single protein in one purification step	561:601	The Bacillus subtilis levansucrase was eluted as a single protein in one purification step.
31407369	6	13	from	50°C	742:745	arg1	incubation					728:737	incubation	728:737	incubation at 50°C for 2 h. Its optimum activity	728:775	It showed its optimum activity at 45°C and could retain 60% of its activity after incubation at 50°C for 2 h. Its optimum activity was obtained at pH 8.2 and the enzyme showed great pH stability in both acidic and alkaline ranges.
31407369	4	14	theme	purification	585:596	arg1	step					598:601	one purification step	581:601	one purification step	581:601	The Bacillus subtilis levansucrase was eluted as a single protein in one purification step.
31407369	8	15	theme	electron	1079:1086	arg1	microscopy					1088:1097	transmission electron microscopy	1066:1097	transmission electron microscopy	1066:1097	The enzyme had antioxidant activities and were characterized as spherical micro- and nanoparticles by transmission electron microscopy.
31407369	2	16	theme	Bacillus	301:308	arg1	isolate					227:233	The most potent isolate	211:233	The most potent isolate that achieved the highest activity (45.66 U/ml)	211:281	The most potent isolate that achieved the highest activity (45.66 U/ml) was identified as Bacillus subtilis NRC based on morphological examination and 16S rRNA.
31407369	2	16	theme	Bacillus	301:308	arg1	NRC					319:321	Bacillus subtilis NRC	301:321	Bacillus subtilis NRC based on morphological examination and 16S rRNA	301:369	The most potent isolate that achieved the highest activity (45.66 U/ml) was identified as Bacillus subtilis NRC based on morphological examination and 16S rRNA.
31407369	7	17	contain	had	922:924	arg2	effect					937:942	an adverse effect	926:942	an adverse effect	926:942	Unlike, most levansucrases all tested metals had an adverse effect in enzyme activity.
31407369	7	17	contain	had	922:924	arg1	metals					915:920	all tested metals	904:920	all tested metals	904:920	Unlike, most levansucrases all tested metals had an adverse effect in enzyme activity.
31407369	3	18	theme	starch	410:415	arg1	necessity					397:405	the necessity	393:405	the necessity of starch (5 g/L), baker's yeast (12.5 g/L), and AlCl3 (5 mM)	393:467	The results recorded the necessity of starch (5 g/L), baker's yeast (12.5 g/L), and AlCl3 (5 mM) in improvement of the enzyme productivity.
31407369	8	19	theme	transmission	1066:1077	arg1	microscopy					1088:1097	transmission electron microscopy	1066:1097	transmission electron microscopy	1066:1097	The enzyme had antioxidant activities and were characterized as spherical micro- and nanoparticles by transmission electron microscopy.
31407369	0	20	theme	new	22:24	arg1	weight					50:55	a new efficient low molecular weight	20:55	a new efficient low molecular weight Bacillus subtilis NRC 16 levansucrase	20:93	Characterization of a new efficient low molecular weight Bacillus subtilis NRC 16 levansucrase and its levan.
31407369	9	21	theme	growth	1114:1119	arg1	conditions					1121:1130	growth conditions	1114:1130	growth conditions	1114:1130	The effect of growth conditions and medium composition in levan structure and its fibrinolytic activity was evaluated.
31407369	7	22	theme	enzyme	947:952	arg1	activity					954:961	enzyme activity	947:961	enzyme activity	947:961	Unlike, most levansucrases all tested metals had an adverse effect in enzyme activity.
31407369	9	23	theme	conditions	1121:1130	arg1	effect					1104:1109	The effect	1100:1109	The effect of growth conditions and medium composition in levan structure and its fibrinolytic activity	1100:1202	The effect of growth conditions and medium composition in levan structure and its fibrinolytic activity was evaluated.
31407369	0	24	dep	Bacillus	57:64	arg1	subtilis					66:73	subtilis	66:73	subtilis	66:73	Characterization of a new efficient low molecular weight Bacillus subtilis NRC 16 levansucrase and its levan.
31407369	4	25	from	protein	570:576	arg1	step					598:601	one purification step	581:601	one purification step	581:601	The Bacillus subtilis levansucrase was eluted as a single protein in one purification step.
31407369	1	26	theme	levansucrase	187:198	arg1	isolates					173:180	all the isolates	165:180	all the isolates	165:180	Screening of 18 bacterial honey isolates revealed that all the isolates were levansucrase producers.
31407369	1	26	theme	levansucrase	187:198	arg1	producers					200:208	levansucrase producers	187:208	levansucrase producers	187:208	Screening of 18 bacterial honey isolates revealed that all the isolates were levansucrase producers.
31407369	0	27	theme	low	36:38	arg1	weight					50:55	a new efficient low molecular weight	20:55	a new efficient low molecular weight Bacillus subtilis NRC 16 levansucrase	20:93	Characterization of a new efficient low molecular weight Bacillus subtilis NRC 16 levansucrase and its levan.
31407369	7	28	theme	adverse	929:935	arg1	effect					937:942	an adverse effect	926:942	an adverse effect	926:942	Unlike, most levansucrases all tested metals had an adverse effect in enzyme activity.
31407369	6	29	theme	optimum	760:766	arg1	activity					768:775	Its optimum activity	756:775	Its optimum activity	756:775	It showed its optimum activity at 45°C and could retain 60% of its activity after incubation at 50°C for 2 h. Its optimum activity was obtained at pH 8.2 and the enzyme showed great pH stability in both acidic and alkaline ranges.
31407369	0	30	dep	weight	50:55	arg1	levansucrase					82:93	Bacillus subtilis NRC 16 levansucrase	57:93	a new efficient low molecular weight Bacillus subtilis NRC 16 levansucrase	20:93	Characterization of a new efficient low molecular weight Bacillus subtilis NRC 16 levansucrase and its levan.
31407369	0	31	theme	efficient	26:34	arg1	weight					50:55	a new efficient low molecular weight	20:55	a new efficient low molecular weight Bacillus subtilis NRC 16 levansucrase	20:93	Characterization of a new efficient low molecular weight Bacillus subtilis NRC 16 levansucrase and its levan.
31407369	4	32	dep	Bacillus	516:523	arg1	subtilis					525:532	subtilis	525:532	subtilis	525:532	The Bacillus subtilis levansucrase was eluted as a single protein in one purification step.
31407369	2	33	theme	potent	220:225	arg1	isolate					227:233	The most potent isolate	211:233	The most potent isolate that achieved the highest activity (45.66 U/ml)	211:281	The most potent isolate that achieved the highest activity (45.66 U/ml) was identified as Bacillus subtilis NRC based on morphological examination and 16S rRNA.
31407369	2	33	theme	potent	220:225	arg1	NRC					319:321	Bacillus subtilis NRC	301:321	Bacillus subtilis NRC based on morphological examination and 16S rRNA	301:369	The most potent isolate that achieved the highest activity (45.66 U/ml) was identified as Bacillus subtilis NRC based on morphological examination and 16S rRNA.
31407369	0	34	theme	weight	50:55	arg1	Characterization					0:15	Characterization	0:15	Characterization of a new efficient low molecular weight Bacillus subtilis NRC 16 levansucrase and its levan.	0:108	Characterization of a new efficient low molecular weight Bacillus subtilis NRC 16 levansucrase and its levan.
31407369	6	35	theme	pH	828:829	arg1	stability					831:839	great pH stability	822:839	great pH stability	822:839	It showed its optimum activity at 45°C and could retain 60% of its activity after incubation at 50°C for 2 h. Its optimum activity was obtained at pH 8.2 and the enzyme showed great pH stability in both acidic and alkaline ranges.
31407369	5	36	theme	enzyme	608:613	arg1	weight					625:630	The enzyme molecular weight	604:630	The enzyme molecular weight	604:630	The enzyme molecular weight was (14 kDa).
31407369	9	37	theme	composition	1143:1153	arg1	effect					1104:1109	The effect	1100:1109	The effect of growth conditions and medium composition in levan structure and its fibrinolytic activity	1100:1202	The effect of growth conditions and medium composition in levan structure and its fibrinolytic activity was evaluated.
31407369	0	38	theme	molecular	40:48	arg1	weight					50:55	a new efficient low molecular weight	20:55	a new efficient low molecular weight Bacillus subtilis NRC 16 levansucrase	20:93	Characterization of a new efficient low molecular weight Bacillus subtilis NRC 16 levansucrase and its levan.
31407369	6	39	theme	great	822:826	arg1	stability					831:839	great pH stability	822:839	great pH stability	822:839	It showed its optimum activity at 45°C and could retain 60% of its activity after incubation at 50°C for 2 h. Its optimum activity was obtained at pH 8.2 and the enzyme showed great pH stability in both acidic and alkaline ranges.
31407369	5	40	theme	molecular	615:623	arg1	weight					625:630	The enzyme molecular weight	604:630	The enzyme molecular weight	604:630	The enzyme molecular weight was (14 kDa).
31407369	9	41	theme	levan	1158:1162	arg1	structure					1164:1172	levan structure	1158:1172	levan structure	1158:1172	The effect of growth conditions and medium composition in levan structure and its fibrinolytic activity was evaluated.
31407369	9	42	theme	medium	1136:1141	arg1	composition					1143:1153	medium composition	1136:1153	medium composition	1136:1153	The effect of growth conditions and medium composition in levan structure and its fibrinolytic activity was evaluated.
31407369	7	43	theme	tested	908:913	arg1	metals					915:920	all tested metals	904:920	all tested metals	904:920	Unlike, most levansucrases all tested metals had an adverse effect in enzyme activity.
31407369	1	44	theme	bacterial	126:134	arg1	isolates					142:149	18 bacterial honey isolates	123:149	18 bacterial honey isolates	123:149	Screening of 18 bacterial honey isolates revealed that all the isolates were levansucrase producers.
31407369	2	45	theme	highest	253:259	arg1	45.66 U/ml					271:280	45.66 U/ml	271:280	45.66 U/ml	271:280	The most potent isolate that achieved the highest activity (45.66 U/ml) was identified as Bacillus subtilis NRC based on morphological examination and 16S rRNA.
31407369	2	45	theme	highest	253:259	arg1	activity					261:268	the highest activity	249:268	the highest activity (45.66 U/ml)	249:281	The most potent isolate that achieved the highest activity (45.66 U/ml) was identified as Bacillus subtilis NRC based on morphological examination and 16S rRNA.
31407369	7	46	theme	most	885:888	arg1	levansucrases					890:902	, most levansucrases	883:902	levansucrases	890:902	Unlike, most levansucrases all tested metals had an adverse effect in enzyme activity.
31407369	2	47	dep	Bacillus	301:308	arg1	subtilis					310:317	subtilis	310:317	subtilis	310:317	The most potent isolate that achieved the highest activity (45.66 U/ml) was identified as Bacillus subtilis NRC based on morphological examination and 16S rRNA.
31407369	6	48	theme	optimum	660:666	arg1	activity					668:675	its optimum activity	656:675	its optimum activity	656:675	It showed its optimum activity at 45°C and could retain 60% of its activity after incubation at 50°C for 2 h. Its optimum activity was obtained at pH 8.2 and the enzyme showed great pH stability in both acidic and alkaline ranges.
31407369	9	49	from	effect	1104:1109	arg1	activity					1195:1202	its fibrinolytic activity	1178:1202	its fibrinolytic activity	1178:1202	The effect of growth conditions and medium composition in levan structure and its fibrinolytic activity was evaluated.
31407369	9	49	from	effect	1104:1109	arg1	structure					1164:1172	levan structure	1158:1172	levan structure	1158:1172	The effect of growth conditions and medium composition in levan structure and its fibrinolytic activity was evaluated.
31407369	8	50	contain	had	975:977	arg2	activities					991:1000	antioxidant activities	979:1000	antioxidant activities	979:1000	The enzyme had antioxidant activities and were characterized as spherical micro- and nanoparticles by transmission electron microscopy.
31407369	8	50	contain	had	975:977	arg1	enzyme					968:973	The enzyme	964:973	The enzyme	964:973	The enzyme had antioxidant activities and were characterized as spherical micro- and nanoparticles by transmission electron microscopy.
31407369	8	50	contain	had	975:977	arg1	nanoparticles					1049:1061	nanoparticles	1049:1061	nanoparticles	1049:1061	The enzyme had antioxidant activities and were characterized as spherical micro- and nanoparticles by transmission electron microscopy.
31407369	8	50	contain	had	975:977	arg1	micro-					1038:1043	spherical micro-	1028:1043	spherical micro-	1028:1043	The enzyme had antioxidant activities and were characterized as spherical micro- and nanoparticles by transmission electron microscopy.
31407369	2	51	theme	16S	362:364	arg1	rRNA					366:369	16S rRNA	362:369	16S rRNA	362:369	The most potent isolate that achieved the highest activity (45.66 U/ml) was identified as Bacillus subtilis NRC based on morphological examination and 16S rRNA.
31407369	0	52	theme	Bacillus	57:64	arg1	levansucrase					82:93	Bacillus subtilis NRC 16 levansucrase	57:93	a new efficient low molecular weight Bacillus subtilis NRC 16 levansucrase	20:93	Characterization of a new efficient low molecular weight Bacillus subtilis NRC 16 levansucrase and its levan.
31407369	1	53	theme	honey	136:140	arg1	isolates					142:149	18 bacterial honey isolates	123:149	18 bacterial honey isolates	123:149	Screening of 18 bacterial honey isolates revealed that all the isolates were levansucrase producers.
31407369	5	54	dep	was	632:634	arg1	14 kDa					637:642	14 kDa	637:642	14 kDa	637:642	The enzyme molecular weight was (14 kDa).
31402925	3	0	theme	cell	603:606	arg1	extraction					613:622	cell wall extraction	603:622	cell wall extraction	603:622	In the present report, we developed and optimized the throughput of five methods to study the diversity in hemp fiber quality including cell wall extraction, biochemical composition of cell wall polysaccharides, quantification of lignin, quantification of crystalline polysaccharides and morphology of the stems.
31402925	2	1	theme	high-throughput	288:302	arg1	methods					316:322	high-throughput phenotyping methods	288:322	high-throughput phenotyping methods that are suited for the analysis of hemp fiber	288:369	Nevertheless, the lack of high-throughput phenotyping methods that are suited for the analysis of hemp fiber, hampers the analysis of many accessions, and consequently the breeding for this complex trait.
31402925	4	2	theme	wall	821:824	arg1	properties					826:835	cell wall properties	816:835	cell wall properties	816:835	Six hemp accessions contrasting for cell wall properties were used to assess the throughput and suitability of these methods for genetic studies.
31402925	6	3	theme	wall	1130:1133	arg1	composition					1135:1145	cell wall composition	1125:1145	cell wall composition	1125:1145	With these methods we were able to detect significant phenotypic variation in cell wall composition and stem morphology between the six accessions.
31402925	3	4	theme	present	474:480	arg1	report					482:487	the present report	470:487	the present report	470:487	In the present report, we developed and optimized the throughput of five methods to study the diversity in hemp fiber quality including cell wall extraction, biochemical composition of cell wall polysaccharides, quantification of lignin, quantification of crystalline polysaccharides and morphology of the stems.
31402925	1	5	theme	fiber	234:238	arg1	quality					218:224	the quality	214:224	the quality of hemp fiber (Cannabis sativa L.)	214:259	The growing demands for sustainable fibers have stimulated the study of genetic diversity in the quality of hemp fiber (Cannabis sativa L.).
31402925	3	6	theme	biochemical	625:635	arg1	composition					637:647	biochemical composition	625:647	biochemical composition of cell wall polysaccharides	625:676	In the present report, we developed and optimized the throughput of five methods to study the diversity in hemp fiber quality including cell wall extraction, biochemical composition of cell wall polysaccharides, quantification of lignin, quantification of crystalline polysaccharides and morphology of the stems.
31402925	1	7	theme	sustainable	145:155	arg1	fibers					157:162	sustainable fibers	145:162	sustainable fibers	145:162	The growing demands for sustainable fibers have stimulated the study of genetic diversity in the quality of hemp fiber (Cannabis sativa L.).
31402925	6	8	theme	phenotypic	1101:1110	arg1	variation					1112:1120	significant phenotypic variation	1089:1120	significant phenotypic variation in cell wall composition and stem morphology between the six accessions	1089:1192	With these methods we were able to detect significant phenotypic variation in cell wall composition and stem morphology between the six accessions.
31402925	3	9	theme	wall	657:660	arg1	polysaccharides					662:676	cell wall polysaccharides	652:676	cell wall polysaccharides	652:676	In the present report, we developed and optimized the throughput of five methods to study the diversity in hemp fiber quality including cell wall extraction, biochemical composition of cell wall polysaccharides, quantification of lignin, quantification of crystalline polysaccharides and morphology of the stems.
31402925	11	10	theme	hemp	1932:1935	arg1	varieties					1937:1945	new hemp varieties	1928:1945	new hemp varieties with advanced quality of fibers	1928:1977	Our methods will enable exploration of genetic diversity of fiber quality and will contribute to the development of new hemp varieties with advanced quality of fibers.
31402925	11	11	theme	advanced	1952:1959	arg1	quality					1961:1967	advanced quality	1952:1967	advanced quality of fibers	1952:1977	Our methods will enable exploration of genetic diversity of fiber quality and will contribute to the development of new hemp varieties with advanced quality of fibers.
31402925	7	12	theme	wall	1311:1314	arg1	traits					1316:1321	cell wall traits	1306:1321	cell wall traits in breeding programs	1306:1342	In addition, the throughput of the methods has been upgraded to a level that enables their use for phenotyping cell wall traits in breeding programs.
31402925	11	13	theme	fiber	1872:1876	arg1	quality					1878:1884	fiber quality	1872:1884	fiber quality	1872:1884	Our methods will enable exploration of genetic diversity of fiber quality and will contribute to the development of new hemp varieties with advanced quality of fibers.
31402925	1	14	from	study	184:188	arg1	quality					218:224	the quality	214:224	the quality of hemp fiber (Cannabis sativa L.)	214:259	The growing demands for sustainable fibers have stimulated the study of genetic diversity in the quality of hemp fiber (Cannabis sativa L.).
31402925	9	15	theme	embedding	1638:1646	arg1	timing					1600:1605	the timing	1596:1605	the timing of fixation, infiltration, and embedding of mature and dry hemp stems	1596:1675	The throughput of the stem morphological analysis was improved by decreasing the timing of fixation, infiltration, and embedding of mature and dry hemp stems.
31402925	10	16	theme	time	1806:1809	arg1	period					1796:1801	a relatively short period	1777:1801	a relatively short period of time	1777:1809	Notwithstanding, our methods already have the potential to phenotype large number of accessions in a relatively short period of time.
31402925	11	17	theme	diversity	1859:1867	arg1	exploration					1836:1846	exploration	1836:1846	exploration of genetic diversity of fiber quality	1836:1884	Our methods will enable exploration of genetic diversity of fiber quality and will contribute to the development of new hemp varieties with advanced quality of fibers.
31402925	9	18	theme	mature	1651:1656	arg1	stems					1671:1675	mature and dry hemp stems	1651:1675	mature and dry hemp stems	1651:1675	The throughput of the stem morphological analysis was improved by decreasing the timing of fixation, infiltration, and embedding of mature and dry hemp stems.
31402925	3	19	theme	polysaccharides	735:749	arg1	extraction					613:622	cell wall extraction	603:622	cell wall extraction	603:622	In the present report, we developed and optimized the throughput of five methods to study the diversity in hemp fiber quality including cell wall extraction, biochemical composition of cell wall polysaccharides, quantification of lignin, quantification of crystalline polysaccharides and morphology of the stems.
31402925	3	19	theme	polysaccharides	735:749	arg1	composition					637:647	biochemical composition	625:647	biochemical composition of cell wall polysaccharides	625:676	In the present report, we developed and optimized the throughput of five methods to study the diversity in hemp fiber quality including cell wall extraction, biochemical composition of cell wall polysaccharides, quantification of lignin, quantification of crystalline polysaccharides and morphology of the stems.
31402925	3	19	theme	polysaccharides	735:749	arg1	quantification					679:692	quantification	679:692	quantification of lignin	679:702	In the present report, we developed and optimized the throughput of five methods to study the diversity in hemp fiber quality including cell wall extraction, biochemical composition of cell wall polysaccharides, quantification of lignin, quantification of crystalline polysaccharides and morphology of the stems.
31402925	3	19	theme	polysaccharides	735:749	arg1	morphology					755:764	morphology	755:764	morphology of the stems	755:777	In the present report, we developed and optimized the throughput of five methods to study the diversity in hemp fiber quality including cell wall extraction, biochemical composition of cell wall polysaccharides, quantification of lignin, quantification of crystalline polysaccharides and morphology of the stems.
31402925	3	19	theme	polysaccharides	735:749	arg1	quantification					705:718	quantification	705:718	quantification of crystalline polysaccharides	705:749	In the present report, we developed and optimized the throughput of five methods to study the diversity in hemp fiber quality including cell wall extraction, biochemical composition of cell wall polysaccharides, quantification of lignin, quantification of crystalline polysaccharides and morphology of the stems.
31402925	5	20	theme	low	987:989	arg1	coefficients					991:1002	low coefficients	987:1002	low coefficients of variation between technical replicates	987:1044	The methods presented revealed to be highly repeatable, with low coefficients of variation between technical replicates.
31402925	10	21	theme	large	1747:1751	arg1	number					1753:1758	phenotype large number	1737:1758	phenotype large number of accessions	1737:1772	Notwithstanding, our methods already have the potential to phenotype large number of accessions in a relatively short period of time.
31402925	9	22	theme	dry	1662:1664	arg1	stems					1671:1675	mature and dry hemp stems	1651:1675	mature and dry hemp stems	1651:1675	The throughput of the stem morphological analysis was improved by decreasing the timing of fixation, infiltration, and embedding of mature and dry hemp stems.
31402925	0	23	theme	Morphology	55:64	arg1	Variation					11:19	Phenotypic Variation	0:19	Phenotypic Variation of Cell Wall Composition and Stem Morphology in Hemp (Cannabis sativa L.): Optimization of Methods.	0:119	Phenotypic Variation of Cell Wall Composition and Stem Morphology in Hemp (Cannabis sativa L.): Optimization of Methods.
31402925	9	24	theme	stems	1671:1675	arg1	timing					1600:1605	the timing	1596:1605	the timing of fixation, infiltration, and embedding of mature and dry hemp stems	1596:1675	The throughput of the stem morphological analysis was improved by decreasing the timing of fixation, infiltration, and embedding of mature and dry hemp stems.
31402925	8	25	theme	complete	1419:1426	arg1	characterization					1428:1443	the complete characterization	1415:1443	the complete characterization of the cell wall of hemp	1415:1468	The cell wall extraction was optimized to extract enough material for the complete characterization of the cell wall of hemp while reducing the time for the entire analysis.
31402925	0	26	theme	Cannabis	75:82	arg1	Hemp					69:72	Hemp	69:72	Hemp (Cannabis sativa L.)	69:93	Phenotypic Variation of Cell Wall Composition and Stem Morphology in Hemp (Cannabis sativa L.): Optimization of Methods.
31402925	0	26	theme	Cannabis	75:82	arg1	L.					91:92	Cannabis sativa L.	75:92	Cannabis sativa L.	75:92	Phenotypic Variation of Cell Wall Composition and Stem Morphology in Hemp (Cannabis sativa L.): Optimization of Methods.
31402925	4	27	theme	methods	897:903	arg1	suitability					876:886	the throughput and suitability	857:886	suitability	876:886	Six hemp accessions contrasting for cell wall properties were used to assess the throughput and suitability of these methods for genetic studies.
31402925	4	27	theme	methods	897:903	arg1	throughput					861:870	the throughput and suitability	857:886	throughput	861:870	Six hemp accessions contrasting for cell wall properties were used to assess the throughput and suitability of these methods for genetic studies.
31402925	8	28	theme	cell	1452:1455	arg1	wall					1457:1460	the cell wall	1448:1460	the cell wall of hemp	1448:1468	The cell wall extraction was optimized to extract enough material for the complete characterization of the cell wall of hemp while reducing the time for the entire analysis.
31402925	6	29	from	variation	1112:1120	arg1	composition					1135:1145	cell wall composition	1125:1145	cell wall composition	1125:1145	With these methods we were able to detect significant phenotypic variation in cell wall composition and stem morphology between the six accessions.
31402925	6	29	from	variation	1112:1120	arg1	morphology					1156:1165	stem morphology	1151:1165	stem morphology	1151:1165	With these methods we were able to detect significant phenotypic variation in cell wall composition and stem morphology between the six accessions.
31402925	4	30	theme	genetic	909:915	arg1	studies					917:923	genetic studies	909:923	genetic studies	909:923	Six hemp accessions contrasting for cell wall properties were used to assess the throughput and suitability of these methods for genetic studies.
31402925	0	31	theme	Phenotypic	0:9	arg1	Variation					11:19	Phenotypic Variation	0:19	Phenotypic Variation of Cell Wall Composition and Stem Morphology in Hemp (Cannabis sativa L.): Optimization of Methods.	0:119	Phenotypic Variation of Cell Wall Composition and Stem Morphology in Hemp (Cannabis sativa L.): Optimization of Methods.
31402925	11	32	theme	quality	1878:1884	arg1	diversity					1859:1867	genetic diversity	1851:1867	genetic diversity of fiber quality	1851:1884	Our methods will enable exploration of genetic diversity of fiber quality and will contribute to the development of new hemp varieties with advanced quality of fibers.
31402925	0	33	dep	Variation	11:19	arg1	Optimization					96:107	Optimization	96:107	Phenotypic Variation of Cell Wall Composition and Stem Morphology in Hemp (Cannabis sativa L.): Optimization of Methods.	0:119	Phenotypic Variation of Cell Wall Composition and Stem Morphology in Hemp (Cannabis sativa L.): Optimization of Methods.
31402925	2	34	theme	fiber	365:369	arg1	analysis					348:355	the analysis	344:355	the analysis of hemp fiber	344:369	Nevertheless, the lack of high-throughput phenotyping methods that are suited for the analysis of hemp fiber, hampers the analysis of many accessions, and consequently the breeding for this complex trait.
31402925	1	35	theme	sativa	250:255	arg1	L					257:257	Cannabis sativa L.	241:258	Cannabis sativa L.	241:258	The growing demands for sustainable fibers have stimulated the study of genetic diversity in the quality of hemp fiber (Cannabis sativa L.).
31402925	1	35	theme	sativa	250:255	arg1	fiber					234:238	hemp fiber	229:238	hemp fiber (Cannabis sativa L.)	229:259	The growing demands for sustainable fibers have stimulated the study of genetic diversity in the quality of hemp fiber (Cannabis sativa L.).
31402925	0	36	theme	Wall	29:32	arg1	Composition					34:44	Cell Wall Composition	24:44	Cell Wall Composition	24:44	Phenotypic Variation of Cell Wall Composition and Stem Morphology in Hemp (Cannabis sativa L.): Optimization of Methods.
31402925	2	37	theme	many	396:399	arg1	accessions					401:410	many accessions	396:410	many accessions	396:410	Nevertheless, the lack of high-throughput phenotyping methods that are suited for the analysis of hemp fiber, hampers the analysis of many accessions, and consequently the breeding for this complex trait.
31402925	8	38	theme	entire	1502:1507	arg1	analysis					1509:1516	the entire analysis	1498:1516	the entire analysis	1498:1516	The cell wall extraction was optimized to extract enough material for the complete characterization of the cell wall of hemp while reducing the time for the entire analysis.
31402925	8	39	theme	cell	1349:1352	arg1	extraction					1359:1368	The cell wall extraction	1345:1368	The cell wall extraction	1345:1368	The cell wall extraction was optimized to extract enough material for the complete characterization of the cell wall of hemp while reducing the time for the entire analysis.
31402925	1	40	theme	genetic	193:199	arg1	diversity					201:209	genetic diversity	193:209	genetic diversity	193:209	The growing demands for sustainable fibers have stimulated the study of genetic diversity in the quality of hemp fiber (Cannabis sativa L.).
31402925	9	41	theme	stem	1541:1544	arg1	analysis					1560:1567	the stem morphological analysis	1537:1567	the stem morphological analysis	1537:1567	The throughput of the stem morphological analysis was improved by decreasing the timing of fixation, infiltration, and embedding of mature and dry hemp stems.
31402925	5	42	dep	technical	1025:1033	arg1	replicates					1035:1044	replicates	1035:1044	replicates	1035:1044	The methods presented revealed to be highly repeatable, with low coefficients of variation between technical replicates.
31402925	3	43	theme	fiber	579:583	arg1	quality					585:591	hemp fiber quality	574:591	hemp fiber quality	574:591	In the present report, we developed and optimized the throughput of five methods to study the diversity in hemp fiber quality including cell wall extraction, biochemical composition of cell wall polysaccharides, quantification of lignin, quantification of crystalline polysaccharides and morphology of the stems.
31402925	9	44	theme	analysis	1560:1567	arg1	throughput					1523:1532	The throughput	1519:1532	The throughput of the stem morphological analysis	1519:1567	The throughput of the stem morphological analysis was improved by decreasing the timing of fixation, infiltration, and embedding of mature and dry hemp stems.
31402925	11	45	with	varieties	1937:1945	arg1	quality					1961:1967	advanced quality	1952:1967	advanced quality of fibers	1952:1977	Our methods will enable exploration of genetic diversity of fiber quality and will contribute to the development of new hemp varieties with advanced quality of fibers.
31402925	2	46	theme	phenotyping	304:314	arg1	methods					316:322	high-throughput phenotyping methods	288:322	high-throughput phenotyping methods that are suited for the analysis of hemp fiber	288:369	Nevertheless, the lack of high-throughput phenotyping methods that are suited for the analysis of hemp fiber, hampers the analysis of many accessions, and consequently the breeding for this complex trait.
31402925	4	47	used	used	842:845	arg2	accessions					789:798	Six hemp accessions	780:798	Six hemp accessions contrasting for cell wall properties	780:835	Six hemp accessions contrasting for cell wall properties were used to assess the throughput and suitability of these methods for genetic studies.
31402925	6	48	with	able	1074:1077	arg1	methods					1058:1064	these methods	1052:1064	these methods	1052:1064	With these methods we were able to detect significant phenotypic variation in cell wall composition and stem morphology between the six accessions.
31402925	3	49	theme	wall	608:611	arg1	extraction					613:622	cell wall extraction	603:622	cell wall extraction	603:622	In the present report, we developed and optimized the throughput of five methods to study the diversity in hemp fiber quality including cell wall extraction, biochemical composition of cell wall polysaccharides, quantification of lignin, quantification of crystalline polysaccharides and morphology of the stems.
31402925	1	50	theme	hemp	229:232	arg1	L					257:257	Cannabis sativa L.	241:258	Cannabis sativa L.	241:258	The growing demands for sustainable fibers have stimulated the study of genetic diversity in the quality of hemp fiber (Cannabis sativa L.).
31402925	1	50	theme	hemp	229:232	arg1	fiber					234:238	hemp fiber	229:238	hemp fiber (Cannabis sativa L.)	229:259	The growing demands for sustainable fibers have stimulated the study of genetic diversity in the quality of hemp fiber (Cannabis sativa L.).
31402925	4	51	theme	cell	816:819	arg1	properties					826:835	cell wall properties	816:835	cell wall properties	816:835	Six hemp accessions contrasting for cell wall properties were used to assess the throughput and suitability of these methods for genetic studies.
31402925	6	52	theme	cell	1125:1128	arg1	composition					1135:1145	cell wall composition	1125:1145	cell wall composition	1125:1145	With these methods we were able to detect significant phenotypic variation in cell wall composition and stem morphology between the six accessions.
31402925	3	53	from	diversity	561:569	arg1	quality					585:591	hemp fiber quality	574:591	hemp fiber quality	574:591	In the present report, we developed and optimized the throughput of five methods to study the diversity in hemp fiber quality including cell wall extraction, biochemical composition of cell wall polysaccharides, quantification of lignin, quantification of crystalline polysaccharides and morphology of the stems.
31402925	3	54	theme	cell	652:655	arg1	polysaccharides					662:676	cell wall polysaccharides	652:676	cell wall polysaccharides	652:676	In the present report, we developed and optimized the throughput of five methods to study the diversity in hemp fiber quality including cell wall extraction, biochemical composition of cell wall polysaccharides, quantification of lignin, quantification of crystalline polysaccharides and morphology of the stems.
31402925	11	55	theme	varieties	1937:1945	arg1	development					1913:1923	the development	1909:1923	the development of new hemp varieties with advanced quality of fibers	1909:1977	Our methods will enable exploration of genetic diversity of fiber quality and will contribute to the development of new hemp varieties with advanced quality of fibers.
31402925	4	56	theme	hemp	784:787	arg1	accessions					789:798	Six hemp accessions	780:798	Six hemp accessions contrasting for cell wall properties	780:835	Six hemp accessions contrasting for cell wall properties were used to assess the throughput and suitability of these methods for genetic studies.
31402925	6	57	theme	significant	1089:1099	arg1	variation					1112:1120	significant phenotypic variation	1089:1120	significant phenotypic variation in cell wall composition and stem morphology between the six accessions	1089:1192	With these methods we were able to detect significant phenotypic variation in cell wall composition and stem morphology between the six accessions.
31402925	11	58	theme	new	1928:1930	arg1	varieties					1937:1945	new hemp varieties	1928:1945	new hemp varieties with advanced quality of fibers	1928:1977	Our methods will enable exploration of genetic diversity of fiber quality and will contribute to the development of new hemp varieties with advanced quality of fibers.
31402925	9	59	theme	infiltration	1620:1631	arg1	timing					1600:1605	the timing	1596:1605	the timing of fixation, infiltration, and embedding of mature and dry hemp stems	1596:1675	The throughput of the stem morphological analysis was improved by decreasing the timing of fixation, infiltration, and embedding of mature and dry hemp stems.
31402925	7	60	theme	breeding	1326:1333	arg1	programs					1335:1342	breeding programs	1326:1342	breeding programs	1326:1342	In addition, the throughput of the methods has been upgraded to a level that enables their use for phenotyping cell wall traits in breeding programs.
31402925	3	61	theme	polysaccharides	662:676	arg1	extraction					613:622	cell wall extraction	603:622	cell wall extraction	603:622	In the present report, we developed and optimized the throughput of five methods to study the diversity in hemp fiber quality including cell wall extraction, biochemical composition of cell wall polysaccharides, quantification of lignin, quantification of crystalline polysaccharides and morphology of the stems.
31402925	3	61	theme	polysaccharides	662:676	arg1	composition					637:647	biochemical composition	625:647	biochemical composition of cell wall polysaccharides	625:676	In the present report, we developed and optimized the throughput of five methods to study the diversity in hemp fiber quality including cell wall extraction, biochemical composition of cell wall polysaccharides, quantification of lignin, quantification of crystalline polysaccharides and morphology of the stems.
31402925	3	61	theme	polysaccharides	662:676	arg1	quantification					679:692	quantification	679:692	quantification of lignin	679:702	In the present report, we developed and optimized the throughput of five methods to study the diversity in hemp fiber quality including cell wall extraction, biochemical composition of cell wall polysaccharides, quantification of lignin, quantification of crystalline polysaccharides and morphology of the stems.
31402925	3	61	theme	polysaccharides	662:676	arg1	morphology					755:764	morphology	755:764	morphology of the stems	755:777	In the present report, we developed and optimized the throughput of five methods to study the diversity in hemp fiber quality including cell wall extraction, biochemical composition of cell wall polysaccharides, quantification of lignin, quantification of crystalline polysaccharides and morphology of the stems.
31402925	3	61	theme	polysaccharides	662:676	arg1	quantification					705:718	quantification	705:718	quantification of crystalline polysaccharides	705:749	In the present report, we developed and optimized the throughput of five methods to study the diversity in hemp fiber quality including cell wall extraction, biochemical composition of cell wall polysaccharides, quantification of lignin, quantification of crystalline polysaccharides and morphology of the stems.
31402925	0	62	theme	Methods	112:118	arg1	Optimization					96:107	Optimization	96:107	Phenotypic Variation of Cell Wall Composition and Stem Morphology in Hemp (Cannabis sativa L.): Optimization of Methods.	0:119	Phenotypic Variation of Cell Wall Composition and Stem Morphology in Hemp (Cannabis sativa L.): Optimization of Methods.
31402925	9	63	theme	fixation	1610:1617	arg1	timing					1600:1605	the timing	1596:1605	the timing of fixation, infiltration, and embedding of mature and dry hemp stems	1596:1675	The throughput of the stem morphological analysis was improved by decreasing the timing of fixation, infiltration, and embedding of mature and dry hemp stems.
31402925	11	64	theme	genetic	1851:1857	arg1	diversity					1859:1867	genetic diversity	1851:1867	genetic diversity of fiber quality	1851:1884	Our methods will enable exploration of genetic diversity of fiber quality and will contribute to the development of new hemp varieties with advanced quality of fibers.
31402925	8	65	theme	material	1402:1409	arg1	extract					1387:1393	extract	1387:1393	extract enough material	1387:1409	The cell wall extraction was optimized to extract enough material for the complete characterization of the cell wall of hemp while reducing the time for the entire analysis.
31402925	7	66	theme	cell	1306:1309	arg1	traits					1316:1321	cell wall traits	1306:1321	cell wall traits in breeding programs	1306:1342	In addition, the throughput of the methods has been upgraded to a level that enables their use for phenotyping cell wall traits in breeding programs.
31402925	3	67	theme	lignin	697:702	arg1	extraction					613:622	cell wall extraction	603:622	cell wall extraction	603:622	In the present report, we developed and optimized the throughput of five methods to study the diversity in hemp fiber quality including cell wall extraction, biochemical composition of cell wall polysaccharides, quantification of lignin, quantification of crystalline polysaccharides and morphology of the stems.
31402925	3	67	theme	lignin	697:702	arg1	composition					637:647	biochemical composition	625:647	biochemical composition of cell wall polysaccharides	625:676	In the present report, we developed and optimized the throughput of five methods to study the diversity in hemp fiber quality including cell wall extraction, biochemical composition of cell wall polysaccharides, quantification of lignin, quantification of crystalline polysaccharides and morphology of the stems.
31402925	3	67	theme	lignin	697:702	arg1	quantification					679:692	quantification	679:692	quantification of lignin	679:702	In the present report, we developed and optimized the throughput of five methods to study the diversity in hemp fiber quality including cell wall extraction, biochemical composition of cell wall polysaccharides, quantification of lignin, quantification of crystalline polysaccharides and morphology of the stems.
31402925	3	67	theme	lignin	697:702	arg1	morphology					755:764	morphology	755:764	morphology of the stems	755:777	In the present report, we developed and optimized the throughput of five methods to study the diversity in hemp fiber quality including cell wall extraction, biochemical composition of cell wall polysaccharides, quantification of lignin, quantification of crystalline polysaccharides and morphology of the stems.
31402925	3	67	theme	lignin	697:702	arg1	quantification					705:718	quantification	705:718	quantification of crystalline polysaccharides	705:749	In the present report, we developed and optimized the throughput of five methods to study the diversity in hemp fiber quality including cell wall extraction, biochemical composition of cell wall polysaccharides, quantification of lignin, quantification of crystalline polysaccharides and morphology of the stems.
31402925	3	68	theme	methods	540:546	arg1	throughput					521:530	the throughput	517:530	the throughput of five methods	517:546	In the present report, we developed and optimized the throughput of five methods to study the diversity in hemp fiber quality including cell wall extraction, biochemical composition of cell wall polysaccharides, quantification of lignin, quantification of crystalline polysaccharides and morphology of the stems.
31402925	6	69	theme	stem	1151:1154	arg1	morphology					1156:1165	stem morphology	1151:1165	stem morphology	1151:1165	With these methods we were able to detect significant phenotypic variation in cell wall composition and stem morphology between the six accessions.
31402925	10	70	theme	phenotype	1737:1745	arg1	number					1753:1758	phenotype large number	1737:1758	phenotype large number of accessions	1737:1772	Notwithstanding, our methods already have the potential to phenotype large number of accessions in a relatively short period of time.
31402925	9	71	theme	hemp	1666:1669	arg1	stems					1671:1675	mature and dry hemp stems	1651:1675	mature and dry hemp stems	1651:1675	The throughput of the stem morphological analysis was improved by decreasing the timing of fixation, infiltration, and embedding of mature and dry hemp stems.
31402925	3	72	theme	crystalline	723:733	arg1	polysaccharides					735:749	crystalline polysaccharides	723:749	crystalline polysaccharides	723:749	In the present report, we developed and optimized the throughput of five methods to study the diversity in hemp fiber quality including cell wall extraction, biochemical composition of cell wall polysaccharides, quantification of lignin, quantification of crystalline polysaccharides and morphology of the stems.
31402925	1	73	theme	growing	125:131	arg1	demands					133:139	The growing demands	121:139	The growing demands for sustainable fibers	121:162	The growing demands for sustainable fibers have stimulated the study of genetic diversity in the quality of hemp fiber (Cannabis sativa L.).
31402925	11	74	theme	fibers	1972:1977	arg1	quality					1961:1967	advanced quality	1952:1967	advanced quality of fibers	1952:1977	Our methods will enable exploration of genetic diversity of fiber quality and will contribute to the development of new hemp varieties with advanced quality of fibers.
31402925	0	75	from	Variation	11:19	arg1	Hemp					69:72	Hemp	69:72	Hemp (Cannabis sativa L.)	69:93	Phenotypic Variation of Cell Wall Composition and Stem Morphology in Hemp (Cannabis sativa L.): Optimization of Methods.
31402925	0	75	from	Variation	11:19	arg1	L.					91:92	Cannabis sativa L.	75:92	Cannabis sativa L.	75:92	Phenotypic Variation of Cell Wall Composition and Stem Morphology in Hemp (Cannabis sativa L.): Optimization of Methods.
31402925	10	76	theme	short	1790:1794	arg1	period					1796:1801	a relatively short period	1777:1801	a relatively short period of time	1777:1809	Notwithstanding, our methods already have the potential to phenotype large number of accessions in a relatively short period of time.
31402925	3	77	theme	stems	773:777	arg1	extraction					613:622	cell wall extraction	603:622	cell wall extraction	603:622	In the present report, we developed and optimized the throughput of five methods to study the diversity in hemp fiber quality including cell wall extraction, biochemical composition of cell wall polysaccharides, quantification of lignin, quantification of crystalline polysaccharides and morphology of the stems.
31402925	3	77	theme	stems	773:777	arg1	composition					637:647	biochemical composition	625:647	biochemical composition of cell wall polysaccharides	625:676	In the present report, we developed and optimized the throughput of five methods to study the diversity in hemp fiber quality including cell wall extraction, biochemical composition of cell wall polysaccharides, quantification of lignin, quantification of crystalline polysaccharides and morphology of the stems.
31402925	3	77	theme	stems	773:777	arg1	quantification					679:692	quantification	679:692	quantification of lignin	679:702	In the present report, we developed and optimized the throughput of five methods to study the diversity in hemp fiber quality including cell wall extraction, biochemical composition of cell wall polysaccharides, quantification of lignin, quantification of crystalline polysaccharides and morphology of the stems.
31402925	3	77	theme	stems	773:777	arg1	morphology					755:764	morphology	755:764	morphology of the stems	755:777	In the present report, we developed and optimized the throughput of five methods to study the diversity in hemp fiber quality including cell wall extraction, biochemical composition of cell wall polysaccharides, quantification of lignin, quantification of crystalline polysaccharides and morphology of the stems.
31402925	3	77	theme	stems	773:777	arg1	quantification					705:718	quantification	705:718	quantification of crystalline polysaccharides	705:749	In the present report, we developed and optimized the throughput of five methods to study the diversity in hemp fiber quality including cell wall extraction, biochemical composition of cell wall polysaccharides, quantification of lignin, quantification of crystalline polysaccharides and morphology of the stems.
31402925	0	78	theme	sativa	84:89	arg1	Hemp					69:72	Hemp	69:72	Hemp (Cannabis sativa L.)	69:93	Phenotypic Variation of Cell Wall Composition and Stem Morphology in Hemp (Cannabis sativa L.): Optimization of Methods.
31402925	0	78	theme	sativa	84:89	arg1	L.					91:92	Cannabis sativa L.	75:92	Cannabis sativa L.	75:92	Phenotypic Variation of Cell Wall Composition and Stem Morphology in Hemp (Cannabis sativa L.): Optimization of Methods.
31402925	2	79	theme	complex	452:458	arg1	trait					460:464	this complex trait	447:464	this complex trait	447:464	Nevertheless, the lack of high-throughput phenotyping methods that are suited for the analysis of hemp fiber, hampers the analysis of many accessions, and consequently the breeding for this complex trait.
31402925	8	80	theme	wall	1457:1460	arg1	characterization					1428:1443	the complete characterization	1415:1443	the complete characterization of the cell wall of hemp	1415:1468	The cell wall extraction was optimized to extract enough material for the complete characterization of the cell wall of hemp while reducing the time for the entire analysis.
31402925	8	81	theme	hemp	1465:1468	arg1	wall					1457:1460	the cell wall	1448:1460	the cell wall of hemp	1448:1468	The cell wall extraction was optimized to extract enough material for the complete characterization of the cell wall of hemp while reducing the time for the entire analysis.
31402925	10	82	contain	have	1715:1718	arg2	potential					1724:1732	the potential	1720:1732	the potential to phenotype large number of accessions	1720:1772	Notwithstanding, our methods already have the potential to phenotype large number of accessions in a relatively short period of time.
31402925	10	82	contain	have	1715:1718	arg1	methods					1699:1705	our methods	1695:1705	our methods	1695:1705	Notwithstanding, our methods already have the potential to phenotype large number of accessions in a relatively short period of time.
31402925	5	83	theme	variation	1007:1015	arg1	coefficients					991:1002	low coefficients	987:1002	low coefficients of variation between technical replicates	987:1044	The methods presented revealed to be highly repeatable, with low coefficients of variation between technical replicates.
31402925	0	84	theme	Cell	24:27	arg1	Composition					34:44	Cell Wall Composition	24:44	Cell Wall Composition	24:44	Phenotypic Variation of Cell Wall Composition and Stem Morphology in Hemp (Cannabis sativa L.): Optimization of Methods.
31402925	1	85	theme	Cannabis	241:248	arg1	L					257:257	Cannabis sativa L.	241:258	Cannabis sativa L.	241:258	The growing demands for sustainable fibers have stimulated the study of genetic diversity in the quality of hemp fiber (Cannabis sativa L.).
31402925	1	85	theme	Cannabis	241:248	arg1	fiber					234:238	hemp fiber	229:238	hemp fiber (Cannabis sativa L.)	229:259	The growing demands for sustainable fibers have stimulated the study of genetic diversity in the quality of hemp fiber (Cannabis sativa L.).
31402925	2	86	theme	hemp	360:363	arg1	fiber					365:369	hemp fiber	360:369	hemp fiber	360:369	Nevertheless, the lack of high-throughput phenotyping methods that are suited for the analysis of hemp fiber, hampers the analysis of many accessions, and consequently the breeding for this complex trait.
31402925	0	87	theme	Composition	34:44	arg1	Variation					11:19	Phenotypic Variation	0:19	Phenotypic Variation of Cell Wall Composition and Stem Morphology in Hemp (Cannabis sativa L.): Optimization of Methods.	0:119	Phenotypic Variation of Cell Wall Composition and Stem Morphology in Hemp (Cannabis sativa L.): Optimization of Methods.
31402925	7	88	from	traits	1316:1321	arg1	programs					1335:1342	breeding programs	1326:1342	breeding programs	1326:1342	In addition, the throughput of the methods has been upgraded to a level that enables their use for phenotyping cell wall traits in breeding programs.
31402925	7	89	theme	methods	1230:1236	arg1	throughput					1212:1221	the throughput	1208:1221	the throughput of the methods	1208:1236	In addition, the throughput of the methods has been upgraded to a level that enables their use for phenotyping cell wall traits in breeding programs.
31402925	2	90	theme	accessions	401:410	arg1	analysis					384:391	the analysis	380:391	the analysis of many accessions	380:410	Nevertheless, the lack of high-throughput phenotyping methods that are suited for the analysis of hemp fiber, hampers the analysis of many accessions, and consequently the breeding for this complex trait.
31402925	10	91	theme	accessions	1763:1772	arg1	number					1753:1758	phenotype large number	1737:1758	phenotype large number of accessions	1737:1772	Notwithstanding, our methods already have the potential to phenotype large number of accessions in a relatively short period of time.
31402925	0	92	theme	Stem	50:53	arg1	Morphology					55:64	Stem Morphology	50:64	Stem Morphology	50:64	Phenotypic Variation of Cell Wall Composition and Stem Morphology in Hemp (Cannabis sativa L.): Optimization of Methods.
31402925	3	93	theme	hemp	574:577	arg1	quality					585:591	hemp fiber quality	574:591	hemp fiber quality	574:591	In the present report, we developed and optimized the throughput of five methods to study the diversity in hemp fiber quality including cell wall extraction, biochemical composition of cell wall polysaccharides, quantification of lignin, quantification of crystalline polysaccharides and morphology of the stems.
31402925	1	94	theme	diversity	201:209	arg1	study					184:188	the study	180:188	the study of genetic diversity in the quality of hemp fiber (Cannabis sativa L.)	180:259	The growing demands for sustainable fibers have stimulated the study of genetic diversity in the quality of hemp fiber (Cannabis sativa L.).
31402925	9	95	theme	morphological	1546:1558	arg1	analysis					1560:1567	the stem morphological analysis	1537:1567	the stem morphological analysis	1537:1567	The throughput of the stem morphological analysis was improved by decreasing the timing of fixation, infiltration, and embedding of mature and dry hemp stems.
31402925	8	96	theme	wall	1354:1357	arg1	extraction					1359:1368	The cell wall extraction	1345:1368	The cell wall extraction	1345:1368	The cell wall extraction was optimized to extract enough material for the complete characterization of the cell wall of hemp while reducing the time for the entire analysis.
31402925	2	97	theme	methods	316:322	arg1	lack					280:283	the lack	276:283	the lack	276:283	Nevertheless, the lack of high-throughput phenotyping methods that are suited for the analysis of hemp fiber, hampers the analysis of many accessions, and consequently the breeding for this complex trait.
30802715	12	0	with	pretreatment	1748:1759	arg1	Ha-EtOAc					1766:1773	Ha-EtOAc	1766:1773	Ha-EtOAc	1766:1773	In addition, pretreatment with Ha-EtOAc could suppress the nuclear translocation of p65 and the phosphorylation of Erk1/2, p38 and JNK.
30802715	7	1	theme	body	1088:1091	arg1	weight					1093:1098	body weight	1088:1098	body weight	1088:1098	The basic indexes of the mice, including body weight, food intake and hematochezia, were recorded during mice experiments.
30802715	10	2	located	detected	1545:1552	arg1	addition					1474:1481	addition	1474:1481	addition	1474:1481	In addition, the impact of Ha-EtOAc on gut microbiota of mice with UC was detected by 16S rDNA sequencing.
30802715	10	2	located	detected	1545:1552	arg2	impact					1488:1493	the impact	1484:1493	the impact of Ha-EtOAc on gut microbiota of mice with UC	1484:1539	In addition, the impact of Ha-EtOAc on gut microbiota of mice with UC was detected by 16S rDNA sequencing.
30802715	15	3	theme	MAPK	2265:2268	arg1	pathways					2270:2277	the NF-κB and MAPK pathways	2251:2277	the NF-κB and MAPK pathways	2251:2277	CONCLUSION Our results demonstrated that Ha-EtOAc exhibited anti-inflammatory effects mainly by suppressing the NF-κB and MAPK pathways, and Ha-EtOAc treatment may be a potent therapy for the treatment of ulcerative colitis.
30802715	10	4	theme	gut	1510:1512	arg1	microbiota					1514:1523	gut microbiota	1510:1523	gut microbiota of mice with UC	1510:1539	In addition, the impact of Ha-EtOAc on gut microbiota of mice with UC was detected by 16S rDNA sequencing.
30802715	0	5	theme	EtOAc	0:4	arg1	extract					6:12	EtOAc extract	0:12	EtOAc extract of H. attenuatum Choisy	0:36	EtOAc extract of H. attenuatum Choisy inhibits inflammation by suppressing the NF-κB and MAPK pathways and modulating the gut microbiota.
30802715	9	6	theme	signaling	1389:1397	arg1	pathways					1399:1406	the NF-κB and MAPK signaling pathways	1370:1406	pathways	1399:1406	Additionally, the influences of Ha-EtOAc on the NF-κB and MAPK signaling pathways were determined by western blot and immunofluorescence assays.
30802715	8	7	theme	cytokines	1220:1228	arg1	levels					1193:1198	The expression levels	1178:1198	The expression levels of pro-inflammatory cytokines, including TNF-α, IL-6 and IL-1β,	1178:1262	METHODS The expression levels of pro-inflammatory cytokines, including TNF-α, IL-6 and IL-1β, were measured by quantitative real-time PCR and western blot.
30802715	13	8	theme	Ha-EtOAc	1871:1878	arg1	treatment					1880:1888	Ha-EtOAc treatment	1871:1888	Ha-EtOAc treatment	1871:1888	Ha-EtOAc treatment ameliorated murine UC, as reflected by a reduced body weight loss, improved colon shortening, alleviated mucosal damage and decreased releases of pro-inflammatory cytokines.
30802715	3	9	theme	H.	567:568	arg1	fractions					554:562	EtOAc fractions	548:562	EtOAc fractions of H. attenuatum Choisy (Ha-EtOAc)	548:597	HYPOTHESIS/PURPOSE In this study, we aimed to investigate the anti-inflammatory mechanisms of EtOAc fractions of H. attenuatum Choisy (Ha-EtOAc) on lipopolysaccharide (LPS)-induced RAW264.7 macrophage inflammation and hypothesized that Ha-EtOAc could attenuate inflammation in the colon.
30802715	1	10	theme	traditional	180:190	arg1	herb					200:203	a traditional Chinese herb	178:203	a traditional Chinese herb	178:203	BACKGROUND Hypericum attenuatum Choisy, a traditional Chinese herb, has been shown to be effective in the treatment of diseases associated with inflammation and has been used to treat rheumatic arthritis in China for centuries.
30802715	1	10	theme	traditional	180:190	arg1	Choisy					170:175	BACKGROUND Hypericum attenuatum Choisy	138:175	BACKGROUND Hypericum attenuatum Choisy	138:175	BACKGROUND Hypericum attenuatum Choisy, a traditional Chinese herb, has been shown to be effective in the treatment of diseases associated with inflammation and has been used to treat rheumatic arthritis in China for centuries.
30802715	15	11	dep	CONCLUSION	2143:2152	arg1	demonstrated					2166:2177	demonstrated	2166:2177	demonstrated that Ha-EtOAc exhibited anti-inflammatory effects mainly by suppressing the NF-κB and MAPK pathways, and Ha-EtOAc treatment may be a potent therapy for the treatment of ulcerative colitis	2166:2365	CONCLUSION Our results demonstrated that Ha-EtOAc exhibited anti-inflammatory effects mainly by suppressing the NF-κB and MAPK pathways, and Ha-EtOAc treatment may be a potent therapy for the treatment of ulcerative colitis.
30802715	11	12	theme	dose-dependent	1712:1725	arg1	manner					1727:1732	a dose-dependent manner	1710:1732	a dose-dependent manner	1710:1732	RESULTS Ha-EtOAc inhibited the LPS-induced production of NO and decreased the release of TNF-α, IL-6 and IL-1β in RAW264.7 cells in a dose-dependent manner.
30802715	1	13	from	treatment	244:252	arg1	effective					227:235	effective	227:235	effective	227:235	BACKGROUND Hypericum attenuatum Choisy, a traditional Chinese herb, has been shown to be effective in the treatment of diseases associated with inflammation and has been used to treat rheumatic arthritis in China for centuries.
30802715	13	14	theme	pro-inflammatory	2036:2051	arg1	cytokines					2053:2061	pro-inflammatory cytokines	2036:2061	pro-inflammatory cytokines	2036:2061	Ha-EtOAc treatment ameliorated murine UC, as reflected by a reduced body weight loss, improved colon shortening, alleviated mucosal damage and decreased releases of pro-inflammatory cytokines.
30802715	3	15	theme	fractions	554:562	arg1	mechanisms					534:543	the anti-inflammatory mechanisms	512:543	the anti-inflammatory mechanisms of EtOAc fractions of H. attenuatum Choisy (Ha-EtOAc) on lipopolysaccharide (LPS)-induced RAW264.7 macrophage inflammation	512:666	HYPOTHESIS/PURPOSE In this study, we aimed to investigate the anti-inflammatory mechanisms of EtOAc fractions of H. attenuatum Choisy (Ha-EtOAc) on lipopolysaccharide (LPS)-induced RAW264.7 macrophage inflammation and hypothesized that Ha-EtOAc could attenuate inflammation in the colon.
30802715	6	16	theme	ulcerative	958:967	arg1	colitis					969:975	Murine ulcerative colitis	951:975	Murine ulcerative colitis (UC)	951:980	Murine ulcerative colitis (UC) was induced by treatment with 2.5% dextran sulfate sodium (DSS).
30802715	6	16	theme	ulcerative	958:967	arg1	UC					978:979	UC	978:979	UC	978:979	Murine ulcerative colitis (UC) was induced by treatment with 2.5% dextran sulfate sodium (DSS).
30802715	3	17	theme	anti-inflammatory	516:532	arg1	mechanisms					534:543	the anti-inflammatory mechanisms	512:543	the anti-inflammatory mechanisms of EtOAc fractions of H. attenuatum Choisy (Ha-EtOAc) on lipopolysaccharide (LPS)-induced RAW264.7 macrophage inflammation	512:666	HYPOTHESIS/PURPOSE In this study, we aimed to investigate the anti-inflammatory mechanisms of EtOAc fractions of H. attenuatum Choisy (Ha-EtOAc) on lipopolysaccharide (LPS)-induced RAW264.7 macrophage inflammation and hypothesized that Ha-EtOAc could attenuate inflammation in the colon.
30802715	10	18	theme	rDNA	1561:1564	arg1	sequencing					1566:1575	rDNA sequencing	1561:1575	rDNA sequencing	1561:1575	In addition, the impact of Ha-EtOAc on gut microbiota of mice with UC was detected by 16S rDNA sequencing.
30802715	0	19	theme	gut	122:124	arg1	microbiota					126:135	the gut microbiota	118:135	the gut microbiota	118:135	EtOAc extract of H. attenuatum Choisy inhibits inflammation by suppressing the NF-κB and MAPK pathways and modulating the gut microbiota.
30802715	0	20	theme	H.	17:18	arg1	extract					6:12	EtOAc extract	0:12	EtOAc extract of H. attenuatum Choisy	0:36	EtOAc extract of H. attenuatum Choisy inhibits inflammation by suppressing the NF-κB and MAPK pathways and modulating the gut microbiota.
30802715	8	21	theme	expression	1182:1191	arg1	levels					1193:1198	The expression levels	1178:1198	The expression levels of pro-inflammatory cytokines, including TNF-α, IL-6 and IL-1β,	1178:1262	METHODS The expression levels of pro-inflammatory cytokines, including TNF-α, IL-6 and IL-1β, were measured by quantitative real-time PCR and western blot.
30802715	15	22	theme	NF-κB	2255:2259	arg1	pathways					2270:2277	the NF-κB and MAPK pathways	2251:2277	the NF-κB and MAPK pathways	2251:2277	CONCLUSION Our results demonstrated that Ha-EtOAc exhibited anti-inflammatory effects mainly by suppressing the NF-κB and MAPK pathways, and Ha-EtOAc treatment may be a potent therapy for the treatment of ulcerative colitis.
30802715	15	23	theme	potent	2312:2317	arg1	therapy					2319:2325	a potent therapy	2310:2325	a potent therapy for the treatment of ulcerative colitis	2310:2365	CONCLUSION Our results demonstrated that Ha-EtOAc exhibited anti-inflammatory effects mainly by suppressing the NF-κB and MAPK pathways, and Ha-EtOAc treatment may be a potent therapy for the treatment of ulcerative colitis.
30802715	15	23	theme	potent	2312:2317	arg1	treatment					2293:2301	Ha-EtOAc treatment	2284:2301	Ha-EtOAc treatment	2284:2301	CONCLUSION Our results demonstrated that Ha-EtOAc exhibited anti-inflammatory effects mainly by suppressing the NF-κB and MAPK pathways, and Ha-EtOAc treatment may be a potent therapy for the treatment of ulcerative colitis.
30802715	5	24	theme	oxide	928:932	arg1	production					939:948	nitric oxide (NO) production	921:948	nitric oxide (NO) production	921:948	The anti-inflammatory effect of Ha-EtOAc in RAW264.7 cells was evaluated by measuring the inhibition ratio of nitric oxide (NO) production.
30802715	1	25	used	used	308:311	arg2	Choisy					170:175	BACKGROUND Hypericum attenuatum Choisy	138:175	BACKGROUND Hypericum attenuatum Choisy	138:175	BACKGROUND Hypericum attenuatum Choisy, a traditional Chinese herb, has been shown to be effective in the treatment of diseases associated with inflammation and has been used to treat rheumatic arthritis in China for centuries.
30802715	1	25	used	used	308:311	arg2	herb					200:203	a traditional Chinese herb	178:203	a traditional Chinese herb	178:203	BACKGROUND Hypericum attenuatum Choisy, a traditional Chinese herb, has been shown to be effective in the treatment of diseases associated with inflammation and has been used to treat rheumatic arthritis in China for centuries.
30802715	11	26	theme	IL-1β	1683:1687	arg1	release					1656:1662	the release	1652:1662	the release of TNF-α, IL-6 and IL-1β in RAW264.7 cells	1652:1705	RESULTS Ha-EtOAc inhibited the LPS-induced production of NO and decreased the release of TNF-α, IL-6 and IL-1β in RAW264.7 cells in a dose-dependent manner.
30802715	6	27	theme	2.5	1012:1014	arg1	%					1015:1015	%	1015:1015	%	1015:1015	Murine ulcerative colitis (UC) was induced by treatment with 2.5% dextran sulfate sodium (DSS).
30802715	11	28	from	release	1656:1662	arg1	cells					1701:1705	RAW264.7 cells	1692:1705	RAW264.7 cells	1692:1705	RESULTS Ha-EtOAc inhibited the LPS-induced production of NO and decreased the release of TNF-α, IL-6 and IL-1β in RAW264.7 cells in a dose-dependent manner.
30802715	11	29	theme	IL-6	1674:1677	arg1	release					1656:1662	the release	1652:1662	the release of TNF-α, IL-6 and IL-1β in RAW264.7 cells	1652:1705	RESULTS Ha-EtOAc inhibited the LPS-induced production of NO and decreased the release of TNF-α, IL-6 and IL-1β in RAW264.7 cells in a dose-dependent manner.
30802715	5	30	theme	inhibition	901:910	arg1	ratio					912:916	the inhibition ratio	897:916	the inhibition ratio of nitric oxide (NO) production	897:948	The anti-inflammatory effect of Ha-EtOAc in RAW264.7 cells was evaluated by measuring the inhibition ratio of nitric oxide (NO) production.
30802715	8	31	theme	quantitative	1281:1292	arg1	PCR					1304:1306	quantitative real-time PCR	1281:1306	quantitative real-time PCR	1281:1306	METHODS The expression levels of pro-inflammatory cytokines, including TNF-α, IL-6 and IL-1β, were measured by quantitative real-time PCR and western blot.
30802715	13	32	theme	mucosal	1995:2001	arg1	damage					2003:2008	mucosal damage	1995:2008	mucosal damage	1995:2008	Ha-EtOAc treatment ameliorated murine UC, as reflected by a reduced body weight loss, improved colon shortening, alleviated mucosal damage and decreased releases of pro-inflammatory cytokines.
30802715	12	33	theme	Erk1/2	1850:1855	arg1	phosphorylation					1831:1845	the phosphorylation	1827:1845	the phosphorylation of Erk1/2, p38 and JNK	1827:1868	In addition, pretreatment with Ha-EtOAc could suppress the nuclear translocation of p65 and the phosphorylation of Erk1/2, p38 and JNK.
30802715	12	33	theme	Erk1/2	1850:1855	arg1	translocation					1802:1814	the nuclear translocation	1790:1814	the nuclear translocation of p65	1790:1821	In addition, pretreatment with Ha-EtOAc could suppress the nuclear translocation of p65 and the phosphorylation of Erk1/2, p38 and JNK.
30802715	1	34	theme	attenuatum	159:168	arg1	herb					200:203	a traditional Chinese herb	178:203	a traditional Chinese herb	178:203	BACKGROUND Hypericum attenuatum Choisy, a traditional Chinese herb, has been shown to be effective in the treatment of diseases associated with inflammation and has been used to treat rheumatic arthritis in China for centuries.
30802715	1	34	theme	attenuatum	159:168	arg1	Choisy					170:175	BACKGROUND Hypericum attenuatum Choisy	138:175	BACKGROUND Hypericum attenuatum Choisy	138:175	BACKGROUND Hypericum attenuatum Choisy, a traditional Chinese herb, has been shown to be effective in the treatment of diseases associated with inflammation and has been used to treat rheumatic arthritis in China for centuries.
30802715	6	35	dep	sulfate	1025:1031	arg1	sodium					1033:1038	sodium	1033:1038	2.5% dextran sulfate sodium (DSS)	1012:1044	Murine ulcerative colitis (UC) was induced by treatment with 2.5% dextran sulfate sodium (DSS).
30802715	6	35	dep	sulfate	1025:1031	arg1	DSS					1041:1043	DSS	1041:1043	DSS	1041:1043	Murine ulcerative colitis (UC) was induced by treatment with 2.5% dextran sulfate sodium (DSS).
30802715	11	36	theme	RAW264.7	1692:1699	arg1	cells					1701:1705	RAW264.7 cells	1692:1705	RAW264.7 cells	1692:1705	RESULTS Ha-EtOAc inhibited the LPS-induced production of NO and decreased the release of TNF-α, IL-6 and IL-1β in RAW264.7 cells in a dose-dependent manner.
30802715	5	37	from	effect	833:838	arg1	cells					864:868	RAW264.7 cells	855:868	RAW264.7 cells	855:868	The anti-inflammatory effect of Ha-EtOAc in RAW264.7 cells was evaluated by measuring the inhibition ratio of nitric oxide (NO) production.
30802715	12	38	theme	p38	1858:1860	arg1	phosphorylation					1831:1845	the phosphorylation	1827:1845	the phosphorylation of Erk1/2, p38 and JNK	1827:1868	In addition, pretreatment with Ha-EtOAc could suppress the nuclear translocation of p65 and the phosphorylation of Erk1/2, p38 and JNK.
30802715	12	38	theme	p38	1858:1860	arg1	translocation					1802:1814	the nuclear translocation	1790:1814	the nuclear translocation of p65	1790:1821	In addition, pretreatment with Ha-EtOAc could suppress the nuclear translocation of p65 and the phosphorylation of Erk1/2, p38 and JNK.
30802715	8	39	theme	western	1312:1318	arg1	blot					1320:1323	western blot	1312:1323	western blot	1312:1323	METHODS The expression levels of pro-inflammatory cytokines, including TNF-α, IL-6 and IL-1β, were measured by quantitative real-time PCR and western blot.
30802715	13	40	theme	colon	1966:1970	arg1	shortening					1972:1981	colon shortening	1966:1981	colon shortening	1966:1981	Ha-EtOAc treatment ameliorated murine UC, as reflected by a reduced body weight loss, improved colon shortening, alleviated mucosal damage and decreased releases of pro-inflammatory cytokines.
30802715	4	41	theme	STUDY	742:746	arg1	LPS					755:757	STUDY DESIGN LPS	742:757	STUDY DESIGN LPS	742:757	STUDY DESIGN LPS was utilized to induce RAW264.7 cells inflammation.
30802715	5	42	theme	anti-inflammatory	815:831	arg1	effect					833:838	The anti-inflammatory effect	811:838	The anti-inflammatory effect of Ha-EtOAc in RAW264.7 cells	811:868	The anti-inflammatory effect of Ha-EtOAc in RAW264.7 cells was evaluated by measuring the inhibition ratio of nitric oxide (NO) production.
30802715	5	43	theme	production	939:948	arg1	ratio					912:916	the inhibition ratio	897:916	the inhibition ratio of nitric oxide (NO) production	897:948	The anti-inflammatory effect of Ha-EtOAc in RAW264.7 cells was evaluated by measuring the inhibition ratio of nitric oxide (NO) production.
30802715	2	44	theme	anti-inflammatory	407:423	arg1	effect					425:430	its anti-inflammatory effect	403:430	its anti-inflammatory effect	403:430	However, the underlying mechanism of its anti-inflammatory effect is poorly understood.
30802715	12	45	theme	nuclear	1794:1800	arg1	translocation					1802:1814	the nuclear translocation	1790:1814	the nuclear translocation of p65	1790:1821	In addition, pretreatment with Ha-EtOAc could suppress the nuclear translocation of p65 and the phosphorylation of Erk1/2, p38 and JNK.
30802715	8	46	dep	METHODS	1170:1176	arg1	measured					1269:1276	measured	1269:1276	were measured by quantitative real-time PCR and western blot	1264:1323	METHODS The expression levels of pro-inflammatory cytokines, including TNF-α, IL-6 and IL-1β, were measured by quantitative real-time PCR and western blot.
30802715	3	47	theme	RAW264.7	635:642	arg1	inflammation					655:666	lipopolysaccharide (LPS)-induced RAW264.7 macrophage inflammation	602:666	lipopolysaccharide (LPS)-induced RAW264.7 macrophage inflammation	602:666	HYPOTHESIS/PURPOSE In this study, we aimed to investigate the anti-inflammatory mechanisms of EtOAc fractions of H. attenuatum Choisy (Ha-EtOAc) on lipopolysaccharide (LPS)-induced RAW264.7 macrophage inflammation and hypothesized that Ha-EtOAc could attenuate inflammation in the colon.
30802715	2	48	theme	underlying	379:388	arg1	mechanism					390:398	the underlying mechanism	375:398	the underlying mechanism of its anti-inflammatory effect	375:430	However, the underlying mechanism of its anti-inflammatory effect is poorly understood.
30802715	6	49	theme	dextran	1017:1023	arg1	sulfate					1025:1031	2.5% dextran sulfate sodium (DSS)	1012:1044	2.5% dextran sulfate sodium (DSS)	1012:1044	Murine ulcerative colitis (UC) was induced by treatment with 2.5% dextran sulfate sodium (DSS).
30802715	13	50	theme	body	1939:1942	arg1	loss					1951:1954	a reduced body weight loss	1929:1954	a reduced body weight loss	1929:1954	Ha-EtOAc treatment ameliorated murine UC, as reflected by a reduced body weight loss, improved colon shortening, alleviated mucosal damage and decreased releases of pro-inflammatory cytokines.
30802715	4	51	theme	cells	791:795	arg1	inflammation					797:808	RAW264.7 cells inflammation	782:808	RAW264.7 cells inflammation	782:808	STUDY DESIGN LPS was utilized to induce RAW264.7 cells inflammation.
30802715	9	52	from	influences	1344:1353	arg1	pathways					1399:1406	the NF-κB and MAPK signaling pathways	1370:1406	pathways	1399:1406	Additionally, the influences of Ha-EtOAc on the NF-κB and MAPK signaling pathways were determined by western blot and immunofluorescence assays.
30802715	9	52	from	influences	1344:1353	arg1	NF-κB					1374:1378	the NF-κB and MAPK signaling pathways	1370:1406	NF-κB	1374:1378	Additionally, the influences of Ha-EtOAc on the NF-κB and MAPK signaling pathways were determined by western blot and immunofluorescence assays.
30802715	1	53	theme	BACKGROUND	138:147	arg1	herb					200:203	a traditional Chinese herb	178:203	a traditional Chinese herb	178:203	BACKGROUND Hypericum attenuatum Choisy, a traditional Chinese herb, has been shown to be effective in the treatment of diseases associated with inflammation and has been used to treat rheumatic arthritis in China for centuries.
30802715	1	53	theme	BACKGROUND	138:147	arg1	Choisy					170:175	BACKGROUND Hypericum attenuatum Choisy	138:175	BACKGROUND Hypericum attenuatum Choisy	138:175	BACKGROUND Hypericum attenuatum Choisy, a traditional Chinese herb, has been shown to be effective in the treatment of diseases associated with inflammation and has been used to treat rheumatic arthritis in China for centuries.
30802715	4	54	used	utilized	763:770	arg2	LPS					755:757	STUDY DESIGN LPS	742:757	STUDY DESIGN LPS	742:757	STUDY DESIGN LPS was utilized to induce RAW264.7 cells inflammation.
30802715	3	55	dep	HYPOTHESIS/PURPOSE	454:471	arg1	study					481:485	this study	476:485	this study	476:485	HYPOTHESIS/PURPOSE In this study, we aimed to investigate the anti-inflammatory mechanisms of EtOAc fractions of H. attenuatum Choisy (Ha-EtOAc) on lipopolysaccharide (LPS)-induced RAW264.7 macrophage inflammation and hypothesized that Ha-EtOAc could attenuate inflammation in the colon.
30802715	14	56	theme	microbial	2120:2128	arg1	communities					2130:2140	microbial communities	2120:2140	microbial communities	2120:2140	Furthermore, Ha-EtOAc could modulate the composition of microbial communities.
30802715	10	57	theme	mice	1528:1531	arg1	microbiota					1514:1523	gut microbiota	1510:1523	gut microbiota of mice with UC	1510:1539	In addition, the impact of Ha-EtOAc on gut microbiota of mice with UC was detected by 16S rDNA sequencing.
30802715	11	58	theme	RESULTS	1578:1584	arg1	Ha-EtOAc					1586:1593	RESULTS Ha-EtOAc	1578:1593	RESULTS Ha-EtOAc	1578:1593	RESULTS Ha-EtOAc inhibited the LPS-induced production of NO and decreased the release of TNF-α, IL-6 and IL-1β in RAW264.7 cells in a dose-dependent manner.
30802715	7	59	theme	mice	1072:1075	arg1	hematochezia					1117:1128	hematochezia	1117:1128	hematochezia	1117:1128	The basic indexes of the mice, including body weight, food intake and hematochezia, were recorded during mice experiments.
30802715	7	59	theme	mice	1072:1075	arg1	weight					1093:1098	body weight	1088:1098	body weight	1088:1098	The basic indexes of the mice, including body weight, food intake and hematochezia, were recorded during mice experiments.
30802715	7	59	theme	mice	1072:1075	arg1	intake					1106:1111	food intake	1101:1111	food intake	1101:1111	The basic indexes of the mice, including body weight, food intake and hematochezia, were recorded during mice experiments.
30802715	7	59	theme	mice	1072:1075	arg1	indexes					1057:1063	The basic indexes	1047:1063	The basic indexes	1047:1063	The basic indexes of the mice, including body weight, food intake and hematochezia, were recorded during mice experiments.
30802715	13	60	theme	murine	1902:1907	arg1	UC					1909:1910	murine UC	1902:1910	murine UC	1902:1910	Ha-EtOAc treatment ameliorated murine UC, as reflected by a reduced body weight loss, improved colon shortening, alleviated mucosal damage and decreased releases of pro-inflammatory cytokines.
30802715	13	61	dep	decreased	2014:2022	arg1	releases					2024:2031	releases	2024:2031	decreased releases of pro-inflammatory cytokines	2014:2061	Ha-EtOAc treatment ameliorated murine UC, as reflected by a reduced body weight loss, improved colon shortening, alleviated mucosal damage and decreased releases of pro-inflammatory cytokines.
30802715	9	62	theme	MAPK	1384:1387	arg1	pathways					1399:1406	the NF-κB and MAPK signaling pathways	1370:1406	pathways	1399:1406	Additionally, the influences of Ha-EtOAc on the NF-κB and MAPK signaling pathways were determined by western blot and immunofluorescence assays.
30802715	3	63	from	inflammation	715:726	arg1	colon					735:739	the colon	731:739	the colon	731:739	HYPOTHESIS/PURPOSE In this study, we aimed to investigate the anti-inflammatory mechanisms of EtOAc fractions of H. attenuatum Choisy (Ha-EtOAc) on lipopolysaccharide (LPS)-induced RAW264.7 macrophage inflammation and hypothesized that Ha-EtOAc could attenuate inflammation in the colon.
30802715	11	64	theme	LPS-induced	1609:1619	arg1	production					1621:1630	the LPS-induced production	1605:1630	the LPS-induced production of NO	1605:1636	RESULTS Ha-EtOAc inhibited the LPS-induced production of NO and decreased the release of TNF-α, IL-6 and IL-1β in RAW264.7 cells in a dose-dependent manner.
30802715	7	65	theme	basic	1051:1055	arg1	hematochezia					1117:1128	hematochezia	1117:1128	hematochezia	1117:1128	The basic indexes of the mice, including body weight, food intake and hematochezia, were recorded during mice experiments.
30802715	7	65	theme	basic	1051:1055	arg1	weight					1093:1098	body weight	1088:1098	body weight	1088:1098	The basic indexes of the mice, including body weight, food intake and hematochezia, were recorded during mice experiments.
30802715	7	65	theme	basic	1051:1055	arg1	intake					1106:1111	food intake	1101:1111	food intake	1101:1111	The basic indexes of the mice, including body weight, food intake and hematochezia, were recorded during mice experiments.
30802715	7	65	theme	basic	1051:1055	arg1	indexes					1057:1063	The basic indexes	1047:1063	The basic indexes	1047:1063	The basic indexes of the mice, including body weight, food intake and hematochezia, were recorded during mice experiments.
30802715	10	66	with	mice	1528:1531	arg1	UC					1538:1539	UC	1538:1539	UC	1538:1539	In addition, the impact of Ha-EtOAc on gut microbiota of mice with UC was detected by 16S rDNA sequencing.
30802715	8	67	theme	pro-inflammatory	1203:1218	arg1	IL-1β					1257:1261	IL-1β	1257:1261	IL-1β	1257:1261	METHODS The expression levels of pro-inflammatory cytokines, including TNF-α, IL-6 and IL-1β, were measured by quantitative real-time PCR and western blot.
30802715	8	67	theme	pro-inflammatory	1203:1218	arg1	IL-6					1248:1251	IL-6	1248:1251	IL-6	1248:1251	METHODS The expression levels of pro-inflammatory cytokines, including TNF-α, IL-6 and IL-1β, were measured by quantitative real-time PCR and western blot.
30802715	8	67	theme	pro-inflammatory	1203:1218	arg1	cytokines					1220:1228	pro-inflammatory cytokines	1203:1228	pro-inflammatory cytokines	1203:1228	METHODS The expression levels of pro-inflammatory cytokines, including TNF-α, IL-6 and IL-1β, were measured by quantitative real-time PCR and western blot.
30802715	8	67	theme	pro-inflammatory	1203:1218	arg1	TNF-α					1241:1245	TNF-α	1241:1245	TNF-α	1241:1245	METHODS The expression levels of pro-inflammatory cytokines, including TNF-α, IL-6 and IL-1β, were measured by quantitative real-time PCR and western blot.
30802715	3	68	theme	EtOAc	548:552	arg1	fractions					554:562	EtOAc fractions	548:562	EtOAc fractions of H. attenuatum Choisy (Ha-EtOAc)	548:597	HYPOTHESIS/PURPOSE In this study, we aimed to investigate the anti-inflammatory mechanisms of EtOAc fractions of H. attenuatum Choisy (Ha-EtOAc) on lipopolysaccharide (LPS)-induced RAW264.7 macrophage inflammation and hypothesized that Ha-EtOAc could attenuate inflammation in the colon.
30802715	1	69	theme	Chinese	192:198	arg1	herb					200:203	a traditional Chinese herb	178:203	a traditional Chinese herb	178:203	BACKGROUND Hypericum attenuatum Choisy, a traditional Chinese herb, has been shown to be effective in the treatment of diseases associated with inflammation and has been used to treat rheumatic arthritis in China for centuries.
30802715	1	69	theme	Chinese	192:198	arg1	Choisy					170:175	BACKGROUND Hypericum attenuatum Choisy	138:175	BACKGROUND Hypericum attenuatum Choisy	138:175	BACKGROUND Hypericum attenuatum Choisy, a traditional Chinese herb, has been shown to be effective in the treatment of diseases associated with inflammation and has been used to treat rheumatic arthritis in China for centuries.
30802715	3	70	dep	H.	567:568	arg1	attenuatum					570:579	H. attenuatum Choisy (Ha-EtOAc)	567:597	H. attenuatum Choisy (Ha-EtOAc)	567:597	HYPOTHESIS/PURPOSE In this study, we aimed to investigate the anti-inflammatory mechanisms of EtOAc fractions of H. attenuatum Choisy (Ha-EtOAc) on lipopolysaccharide (LPS)-induced RAW264.7 macrophage inflammation and hypothesized that Ha-EtOAc could attenuate inflammation in the colon.
30802715	3	70	dep	H.	567:568	arg1	Ha-EtOAc					589:596	Ha-EtOAc	589:596	Ha-EtOAc	589:596	HYPOTHESIS/PURPOSE In this study, we aimed to investigate the anti-inflammatory mechanisms of EtOAc fractions of H. attenuatum Choisy (Ha-EtOAc) on lipopolysaccharide (LPS)-induced RAW264.7 macrophage inflammation and hypothesized that Ha-EtOAc could attenuate inflammation in the colon.
30802715	3	70	dep	H.	567:568	arg1	Choisy					581:586	Choisy	581:586	Choisy	581:586	HYPOTHESIS/PURPOSE In this study, we aimed to investigate the anti-inflammatory mechanisms of EtOAc fractions of H. attenuatum Choisy (Ha-EtOAc) on lipopolysaccharide (LPS)-induced RAW264.7 macrophage inflammation and hypothesized that Ha-EtOAc could attenuate inflammation in the colon.
30802715	1	71	from	effective	227:235	arg1	treatment					244:252	the treatment	240:252	the treatment of diseases associated with inflammation	240:293	BACKGROUND Hypericum attenuatum Choisy, a traditional Chinese herb, has been shown to be effective in the treatment of diseases associated with inflammation and has been used to treat rheumatic arthritis in China for centuries.
30802715	5	72	theme	RAW264.7	855:862	arg1	cells					864:868	RAW264.7 cells	855:868	RAW264.7 cells	855:868	The anti-inflammatory effect of Ha-EtOAc in RAW264.7 cells was evaluated by measuring the inhibition ratio of nitric oxide (NO) production.
30802715	12	73	theme	JNK	1866:1868	arg1	phosphorylation					1831:1845	the phosphorylation	1827:1845	the phosphorylation of Erk1/2, p38 and JNK	1827:1868	In addition, pretreatment with Ha-EtOAc could suppress the nuclear translocation of p65 and the phosphorylation of Erk1/2, p38 and JNK.
30802715	12	73	theme	JNK	1866:1868	arg1	translocation					1802:1814	the nuclear translocation	1790:1814	the nuclear translocation of p65	1790:1821	In addition, pretreatment with Ha-EtOAc could suppress the nuclear translocation of p65 and the phosphorylation of Erk1/2, p38 and JNK.
30802715	6	74	theme	Murine	951:956	arg1	colitis					969:975	Murine ulcerative colitis	951:975	Murine ulcerative colitis (UC)	951:980	Murine ulcerative colitis (UC) was induced by treatment with 2.5% dextran sulfate sodium (DSS).
30802715	6	74	theme	Murine	951:956	arg1	UC					978:979	UC	978:979	UC	978:979	Murine ulcerative colitis (UC) was induced by treatment with 2.5% dextran sulfate sodium (DSS).
30802715	6	75	with	treatment	997:1005	arg1	sulfate					1025:1031	2.5% dextran sulfate sodium (DSS)	1012:1044	2.5% dextran sulfate sodium (DSS)	1012:1044	Murine ulcerative colitis (UC) was induced by treatment with 2.5% dextran sulfate sodium (DSS).
30802715	7	76	theme	mice	1152:1155	arg1	experiments					1157:1167	mice experiments	1152:1167	mice experiments	1152:1167	The basic indexes of the mice, including body weight, food intake and hematochezia, were recorded during mice experiments.
30802715	5	77	theme	nitric	921:926	arg1	oxide					928:932	nitric oxide	921:932	nitric oxide (NO) production	921:948	The anti-inflammatory effect of Ha-EtOAc in RAW264.7 cells was evaluated by measuring the inhibition ratio of nitric oxide (NO) production.
30802715	5	77	theme	nitric	921:926	arg1	NO					935:936	NO	935:936	NO	935:936	The anti-inflammatory effect of Ha-EtOAc in RAW264.7 cells was evaluated by measuring the inhibition ratio of nitric oxide (NO) production.
30802715	6	78	theme	%	1015:1015	arg1	sulfate					1025:1031	2.5% dextran sulfate sodium (DSS)	1012:1044	2.5% dextran sulfate sodium (DSS)	1012:1044	Murine ulcerative colitis (UC) was induced by treatment with 2.5% dextran sulfate sodium (DSS).
30802715	5	79	theme	Ha-EtOAc	843:850	arg1	effect					833:838	The anti-inflammatory effect	811:838	The anti-inflammatory effect of Ha-EtOAc in RAW264.7 cells	811:868	The anti-inflammatory effect of Ha-EtOAc in RAW264.7 cells was evaluated by measuring the inhibition ratio of nitric oxide (NO) production.
30802715	0	80	theme	NF-κB	79:83	arg1	pathways					94:101	the NF-κB and MAPK pathways	75:101	pathways	94:101	EtOAc extract of H. attenuatum Choisy inhibits inflammation by suppressing the NF-κB and MAPK pathways and modulating the gut microbiota.
30802715	1	81	theme	Hypericum	149:157	arg1	herb					200:203	a traditional Chinese herb	178:203	a traditional Chinese herb	178:203	BACKGROUND Hypericum attenuatum Choisy, a traditional Chinese herb, has been shown to be effective in the treatment of diseases associated with inflammation and has been used to treat rheumatic arthritis in China for centuries.
30802715	1	81	theme	Hypericum	149:157	arg1	Choisy					170:175	BACKGROUND Hypericum attenuatum Choisy	138:175	BACKGROUND Hypericum attenuatum Choisy	138:175	BACKGROUND Hypericum attenuatum Choisy, a traditional Chinese herb, has been shown to be effective in the treatment of diseases associated with inflammation and has been used to treat rheumatic arthritis in China for centuries.
30802715	3	82	from	mechanisms	534:543	arg1	inflammation					655:666	lipopolysaccharide (LPS)-induced RAW264.7 macrophage inflammation	602:666	lipopolysaccharide (LPS)-induced RAW264.7 macrophage inflammation	602:666	HYPOTHESIS/PURPOSE In this study, we aimed to investigate the anti-inflammatory mechanisms of EtOAc fractions of H. attenuatum Choisy (Ha-EtOAc) on lipopolysaccharide (LPS)-induced RAW264.7 macrophage inflammation and hypothesized that Ha-EtOAc could attenuate inflammation in the colon.
30802715	0	83	theme	MAPK	89:92	arg1	pathways					94:101	the NF-κB and MAPK pathways	75:101	pathways	94:101	EtOAc extract of H. attenuatum Choisy inhibits inflammation by suppressing the NF-κB and MAPK pathways and modulating the gut microbiota.
30802715	1	84	theme	rheumatic	322:330	arg1	arthritis					332:340	rheumatic arthritis	322:340	rheumatic arthritis in China for centuries	322:363	BACKGROUND Hypericum attenuatum Choisy, a traditional Chinese herb, has been shown to be effective in the treatment of diseases associated with inflammation and has been used to treat rheumatic arthritis in China for centuries.
30802715	8	85	theme	real-time	1294:1302	arg1	PCR					1304:1306	quantitative real-time PCR	1281:1306	quantitative real-time PCR	1281:1306	METHODS The expression levels of pro-inflammatory cytokines, including TNF-α, IL-6 and IL-1β, were measured by quantitative real-time PCR and western blot.
30802715	9	86	theme	immunofluorescence	1444:1461	arg1	assays					1463:1468	immunofluorescence assays	1444:1468	immunofluorescence assays	1444:1468	Additionally, the influences of Ha-EtOAc on the NF-κB and MAPK signaling pathways were determined by western blot and immunofluorescence assays.
30802715	14	87	theme	communities	2130:2140	arg1	composition					2105:2115	the composition	2101:2115	the composition of microbial communities	2101:2140	Furthermore, Ha-EtOAc could modulate the composition of microbial communities.
30802715	15	88	theme	colitis	2359:2365	arg1	treatment					2335:2343	the treatment	2331:2343	the treatment of ulcerative colitis	2331:2365	CONCLUSION Our results demonstrated that Ha-EtOAc exhibited anti-inflammatory effects mainly by suppressing the NF-κB and MAPK pathways, and Ha-EtOAc treatment may be a potent therapy for the treatment of ulcerative colitis.
30802715	2	89	theme	effect	425:430	arg1	mechanism					390:398	the underlying mechanism	375:398	the underlying mechanism of its anti-inflammatory effect	375:430	However, the underlying mechanism of its anti-inflammatory effect is poorly understood.
30802715	1	90	from	arthritis	332:340	arg1	China					345:349	China	345:349	China	345:349	BACKGROUND Hypericum attenuatum Choisy, a traditional Chinese herb, has been shown to be effective in the treatment of diseases associated with inflammation and has been used to treat rheumatic arthritis in China for centuries.
30802715	1	91	theme	diseases	257:264	arg1	treatment					244:252	the treatment	240:252	the treatment of diseases associated with inflammation	240:293	BACKGROUND Hypericum attenuatum Choisy, a traditional Chinese herb, has been shown to be effective in the treatment of diseases associated with inflammation and has been used to treat rheumatic arthritis in China for centuries.
30802715	3	92	theme	-induced	626:633	arg1	inflammation					655:666	lipopolysaccharide (LPS)-induced RAW264.7 macrophage inflammation	602:666	lipopolysaccharide (LPS)-induced RAW264.7 macrophage inflammation	602:666	HYPOTHESIS/PURPOSE In this study, we aimed to investigate the anti-inflammatory mechanisms of EtOAc fractions of H. attenuatum Choisy (Ha-EtOAc) on lipopolysaccharide (LPS)-induced RAW264.7 macrophage inflammation and hypothesized that Ha-EtOAc could attenuate inflammation in the colon.
30802715	4	93	theme	DESIGN	748:753	arg1	LPS					755:757	STUDY DESIGN LPS	742:757	STUDY DESIGN LPS	742:757	STUDY DESIGN LPS was utilized to induce RAW264.7 cells inflammation.
30802715	7	94	theme	food	1101:1104	arg1	intake					1106:1111	food intake	1101:1111	food intake	1101:1111	The basic indexes of the mice, including body weight, food intake and hematochezia, were recorded during mice experiments.
30802715	12	95	theme	p65	1819:1821	arg1	phosphorylation					1831:1845	the phosphorylation	1827:1845	the phosphorylation of Erk1/2, p38 and JNK	1827:1868	In addition, pretreatment with Ha-EtOAc could suppress the nuclear translocation of p65 and the phosphorylation of Erk1/2, p38 and JNK.
30802715	12	95	theme	p65	1819:1821	arg1	translocation					1802:1814	the nuclear translocation	1790:1814	the nuclear translocation of p65	1790:1821	In addition, pretreatment with Ha-EtOAc could suppress the nuclear translocation of p65 and the phosphorylation of Erk1/2, p38 and JNK.
30802715	10	96	from	impact	1488:1493	arg1	microbiota					1514:1523	gut microbiota	1510:1523	gut microbiota of mice with UC	1510:1539	In addition, the impact of Ha-EtOAc on gut microbiota of mice with UC was detected by 16S rDNA sequencing.
30802715	11	97	theme	NO	1635:1636	arg1	production					1621:1630	the LPS-induced production	1605:1630	the LPS-induced production of NO	1605:1636	RESULTS Ha-EtOAc inhibited the LPS-induced production of NO and decreased the release of TNF-α, IL-6 and IL-1β in RAW264.7 cells in a dose-dependent manner.
30802715	13	98	theme	reduced	1931:1937	arg1	loss					1951:1954	a reduced body weight loss	1929:1954	a reduced body weight loss	1929:1954	Ha-EtOAc treatment ameliorated murine UC, as reflected by a reduced body weight loss, improved colon shortening, alleviated mucosal damage and decreased releases of pro-inflammatory cytokines.
30802715	9	99	theme	western	1427:1433	arg1	blot					1435:1438	western blot	1427:1438	western blot	1427:1438	Additionally, the influences of Ha-EtOAc on the NF-κB and MAPK signaling pathways were determined by western blot and immunofluorescence assays.
30802715	15	100	theme	ulcerative	2348:2357	arg1	colitis					2359:2365	ulcerative colitis	2348:2365	ulcerative colitis	2348:2365	CONCLUSION Our results demonstrated that Ha-EtOAc exhibited anti-inflammatory effects mainly by suppressing the NF-κB and MAPK pathways, and Ha-EtOAc treatment may be a potent therapy for the treatment of ulcerative colitis.
30802715	11	101	theme	TNF-α	1667:1671	arg1	release					1656:1662	the release	1652:1662	the release of TNF-α, IL-6 and IL-1β in RAW264.7 cells	1652:1705	RESULTS Ha-EtOAc inhibited the LPS-induced production of NO and decreased the release of TNF-α, IL-6 and IL-1β in RAW264.7 cells in a dose-dependent manner.
30802715	13	102	theme	weight	1944:1949	arg1	loss					1951:1954	a reduced body weight loss	1929:1954	a reduced body weight loss	1929:1954	Ha-EtOAc treatment ameliorated murine UC, as reflected by a reduced body weight loss, improved colon shortening, alleviated mucosal damage and decreased releases of pro-inflammatory cytokines.
30802715	15	103	theme	anti-inflammatory	2203:2219	arg1	effects					2221:2227	anti-inflammatory effects	2203:2227	anti-inflammatory effects	2203:2227	CONCLUSION Our results demonstrated that Ha-EtOAc exhibited anti-inflammatory effects mainly by suppressing the NF-κB and MAPK pathways, and Ha-EtOAc treatment may be a potent therapy for the treatment of ulcerative colitis.
30802715	0	104	dep	H.	17:18	arg1	Choisy					31:36	Choisy	31:36	Choisy	31:36	EtOAc extract of H. attenuatum Choisy inhibits inflammation by suppressing the NF-κB and MAPK pathways and modulating the gut microbiota.
30802715	0	104	dep	H.	17:18	arg1	attenuatum					20:29	H. attenuatum Choisy	17:36	H. attenuatum Choisy	17:36	EtOAc extract of H. attenuatum Choisy inhibits inflammation by suppressing the NF-κB and MAPK pathways and modulating the gut microbiota.
30802715	3	105	theme	macrophage	644:653	arg1	inflammation					655:666	lipopolysaccharide (LPS)-induced RAW264.7 macrophage inflammation	602:666	lipopolysaccharide (LPS)-induced RAW264.7 macrophage inflammation	602:666	HYPOTHESIS/PURPOSE In this study, we aimed to investigate the anti-inflammatory mechanisms of EtOAc fractions of H. attenuatum Choisy (Ha-EtOAc) on lipopolysaccharide (LPS)-induced RAW264.7 macrophage inflammation and hypothesized that Ha-EtOAc could attenuate inflammation in the colon.
30802715	4	106	theme	RAW264.7	782:789	arg1	cells					791:795	RAW264.7 cells	782:795	RAW264.7 cells inflammation	782:808	STUDY DESIGN LPS was utilized to induce RAW264.7 cells inflammation.
30802715	10	107	theme	Ha-EtOAc	1498:1505	arg1	impact					1488:1493	the impact	1484:1493	the impact of Ha-EtOAc on gut microbiota of mice with UC	1484:1539	In addition, the impact of Ha-EtOAc on gut microbiota of mice with UC was detected by 16S rDNA sequencing.
30802715	15	108	theme	Ha-EtOAc	2284:2291	arg1	therapy					2319:2325	a potent therapy	2310:2325	a potent therapy for the treatment of ulcerative colitis	2310:2365	CONCLUSION Our results demonstrated that Ha-EtOAc exhibited anti-inflammatory effects mainly by suppressing the NF-κB and MAPK pathways, and Ha-EtOAc treatment may be a potent therapy for the treatment of ulcerative colitis.
30802715	15	108	theme	Ha-EtOAc	2284:2291	arg1	treatment					2293:2301	Ha-EtOAc treatment	2284:2301	Ha-EtOAc treatment	2284:2301	CONCLUSION Our results demonstrated that Ha-EtOAc exhibited anti-inflammatory effects mainly by suppressing the NF-κB and MAPK pathways, and Ha-EtOAc treatment may be a potent therapy for the treatment of ulcerative colitis.
30802715	9	109	theme	Ha-EtOAc	1358:1365	arg1	influences					1344:1353	the influences	1340:1353	the influences of Ha-EtOAc on the NF-κB and MAPK signaling pathways	1340:1406	Additionally, the influences of Ha-EtOAc on the NF-κB and MAPK signaling pathways were determined by western blot and immunofluorescence assays.
30712100	5	0	theme	overall	866:872	arg1	impression					874:883	their overall impression	860:883	their overall impression	860:883	Fermentations with the highest concentration of hydrolyzed mannose showed the highest mouthfeel properties, but the lack of acidity reduced their overall impression.
30712100	0	1	from	fermentation	16:27	arg1	Composition					148:158	mannose-containing polysaccharides wine Composition	108:158	mannose-containing polysaccharides wine Composition	108:158	Mixed alcoholic fermentation of Schizosaccharomyces pombe and Lachancea thermotolerans and its influence on mannose-containing polysaccharides wine Composition.
30712100	5	2	theme	highest	743:749	arg1	concentration					751:763	the highest concentration	739:763	the highest concentration of hydrolyzed mannose	739:785	Fermentations with the highest concentration of hydrolyzed mannose showed the highest mouthfeel properties, but the lack of acidity reduced their overall impression.
30712100	1	3	theme	winemaking	187:196	arg1	performance					198:208	the winemaking performance	183:208	the winemaking performance of new biotechnology involving the cooperation of Lachancea and Schizosaccharomyces genera in the production of wine	183:325	This study researched the winemaking performance of new biotechnology involving the cooperation of Lachancea and Schizosaccharomyces genera in the production of wine.
30712100	5	4	theme	acidity	844:850	arg1	lack					836:839	the lack	832:839	the lack of acidity	832:850	Fermentations with the highest concentration of hydrolyzed mannose showed the highest mouthfeel properties, but the lack of acidity reduced their overall impression.
30712100	2	5	theme	acid	408:411	arg1	concentrations					413:426	higher lactic acid concentrations	394:426	higher lactic acid concentrations	394:426	In all fermentations where Lachancea thermotolerans was involved, higher lactic acid concentrations appeared, while all fermentations where Schizosaccharomyces pombe was involved, lower levels in malic acid concentration took place.
30712100	6	6	theme	overall	969:975	arg1	impression					977:986	the highest overall impression	957:986	the highest overall impression	957:986	Wines made from a combination of L. thermotolerans and S. pombe showed the highest overall impression and were preferred by the tasters due to the balance between mouthfeel properties and acidity.
30712100	0	7	theme	mannose-containing	108:125	arg1	Composition					148:158	mannose-containing polysaccharides wine Composition	108:158	mannose-containing polysaccharides wine Composition	108:158	Mixed alcoholic fermentation of Schizosaccharomyces pombe and Lachancea thermotolerans and its influence on mannose-containing polysaccharides wine Composition.
30712100	2	8	from	levels	514:519	arg1	concentration					535:547	malic acid concentration	524:547	malic acid concentration	524:547	In all fermentations where Lachancea thermotolerans was involved, higher lactic acid concentrations appeared, while all fermentations where Schizosaccharomyces pombe was involved, lower levels in malic acid concentration took place.
30712100	5	9	theme	hydrolyzed	768:777	arg1	mannose					779:785	hydrolyzed mannose	768:785	hydrolyzed mannose	768:785	Fermentations with the highest concentration of hydrolyzed mannose showed the highest mouthfeel properties, but the lack of acidity reduced their overall impression.
30712100	1	10	theme	wine	322:325	arg1	production					308:317	the production	304:317	the production of wine	304:325	This study researched the winemaking performance of new biotechnology involving the cooperation of Lachancea and Schizosaccharomyces genera in the production of wine.
30712100	0	11	theme	wine	143:146	arg1	Composition					148:158	mannose-containing polysaccharides wine Composition	108:158	mannose-containing polysaccharides wine Composition	108:158	Mixed alcoholic fermentation of Schizosaccharomyces pombe and Lachancea thermotolerans and its influence on mannose-containing polysaccharides wine Composition.
30712100	3	12	theme	wines	599:603	arg1	properties					575:584	The sensorial properties	561:584	The sensorial properties of the final wines	561:603	The sensorial properties of the final wines varied accordingly.
30712100	0	13	theme	polysaccharides	127:141	arg1	Composition					148:158	mannose-containing polysaccharides wine Composition	108:158	mannose-containing polysaccharides wine Composition	108:158	Mixed alcoholic fermentation of Schizosaccharomyces pombe and Lachancea thermotolerans and its influence on mannose-containing polysaccharides wine Composition.
30712100	1	14	theme	new	213:215	arg1	biotechnology					217:229	new biotechnology	213:229	new biotechnology involving the cooperation of Lachancea and Schizosaccharomyces genera in the production of wine	213:325	This study researched the winemaking performance of new biotechnology involving the cooperation of Lachancea and Schizosaccharomyces genera in the production of wine.
30712100	0	15	theme	alcoholic	6:14	arg1	fermentation					16:27	Mixed alcoholic fermentation	0:27	Mixed alcoholic fermentation of Schizosaccharomyces pombe and Lachancea thermotolerans	0:85	Mixed alcoholic fermentation of Schizosaccharomyces pombe and Lachancea thermotolerans and its influence on mannose-containing polysaccharides wine Composition.
30712100	4	16	theme	mouthfeel	640:648	arg1	properties					650:659	mouthfeel properties	640:659	mouthfeel properties	640:659	Differences in mouthfeel properties and acidity occurred in the different fermentation trials.
30712100	1	17	theme	biotechnology	217:229	arg1	performance					198:208	the winemaking performance	183:208	the winemaking performance of new biotechnology involving the cooperation of Lachancea and Schizosaccharomyces genera in the production of wine	183:325	This study researched the winemaking performance of new biotechnology involving the cooperation of Lachancea and Schizosaccharomyces genera in the production of wine.
30712100	0	18	theme	Mixed	0:4	arg1	fermentation					16:27	Mixed alcoholic fermentation	0:27	Mixed alcoholic fermentation of Schizosaccharomyces pombe and Lachancea thermotolerans	0:85	Mixed alcoholic fermentation of Schizosaccharomyces pombe and Lachancea thermotolerans and its influence on mannose-containing polysaccharides wine Composition.
30712100	2	19	theme	lower	508:512	arg1	levels					514:519	lower levels	508:519	lower levels in malic acid concentration	508:547	In all fermentations where Lachancea thermotolerans was involved, higher lactic acid concentrations appeared, while all fermentations where Schizosaccharomyces pombe was involved, lower levels in malic acid concentration took place.
30712100	2	19	theme	lower	508:512	arg1	fermentations					448:460	all fermentations	444:460	all fermentations where Schizosaccharomyces pombe was involved	444:505	In all fermentations where Lachancea thermotolerans was involved, higher lactic acid concentrations appeared, while all fermentations where Schizosaccharomyces pombe was involved, lower levels in malic acid concentration took place.
30712100	5	20	theme	mouthfeel	806:814	arg1	properties					816:825	the highest mouthfeel properties	794:825	the highest mouthfeel properties	794:825	Fermentations with the highest concentration of hydrolyzed mannose showed the highest mouthfeel properties, but the lack of acidity reduced their overall impression.
30712100	2	21	theme	Lachancea	355:363	arg1	thermotolerans					365:378	Lachancea thermotolerans	355:378	Lachancea thermotolerans	355:378	In all fermentations where Lachancea thermotolerans was involved, higher lactic acid concentrations appeared, while all fermentations where Schizosaccharomyces pombe was involved, lower levels in malic acid concentration took place.
30712100	4	22	from	Differences	625:635	arg1	acidity					665:671	acidity	665:671	acidity	665:671	Differences in mouthfeel properties and acidity occurred in the different fermentation trials.
30712100	4	22	from	Differences	625:635	arg1	properties					650:659	mouthfeel properties	640:659	mouthfeel properties	640:659	Differences in mouthfeel properties and acidity occurred in the different fermentation trials.
30712100	0	23	from	influence	95:103	arg1	Composition					148:158	mannose-containing polysaccharides wine Composition	108:158	mannose-containing polysaccharides wine Composition	108:158	Mixed alcoholic fermentation of Schizosaccharomyces pombe and Lachancea thermotolerans and its influence on mannose-containing polysaccharides wine Composition.
30712100	0	24	theme	pombe	52:56	arg1	fermentation					16:27	Mixed alcoholic fermentation	0:27	Mixed alcoholic fermentation of Schizosaccharomyces pombe and Lachancea thermotolerans	0:85	Mixed alcoholic fermentation of Schizosaccharomyces pombe and Lachancea thermotolerans and its influence on mannose-containing polysaccharides wine Composition.
30712100	0	24	theme	pombe	52:56	arg1	influence					95:103	its influence	91:103	its influence on mannose-containing polysaccharides wine Composition	91:158	Mixed alcoholic fermentation of Schizosaccharomyces pombe and Lachancea thermotolerans and its influence on mannose-containing polysaccharides wine Composition.
30712100	3	25	theme	sensorial	565:573	arg1	properties					575:584	The sensorial properties	561:584	The sensorial properties of the final wines	561:603	The sensorial properties of the final wines varied accordingly.
30712100	6	26	theme	highest	961:967	arg1	impression					977:986	the highest overall impression	957:986	the highest overall impression	957:986	Wines made from a combination of L. thermotolerans and S. pombe showed the highest overall impression and were preferred by the tasters due to the balance between mouthfeel properties and acidity.
30712100	6	27	theme	mouthfeel	1049:1057	arg1	properties					1059:1068	mouthfeel properties	1049:1068	mouthfeel properties	1049:1068	Wines made from a combination of L. thermotolerans and S. pombe showed the highest overall impression and were preferred by the tasters due to the balance between mouthfeel properties and acidity.
30712100	1	28	theme	Lachancea	260:268	arg1	cooperation					245:255	the cooperation	241:255	the cooperation of Lachancea and Schizosaccharomyces genera in the production of wine	241:325	This study researched the winemaking performance of new biotechnology involving the cooperation of Lachancea and Schizosaccharomyces genera in the production of wine.
30712100	2	29	theme	acid	530:533	arg1	concentration					535:547	malic acid concentration	524:547	malic acid concentration	524:547	In all fermentations where Lachancea thermotolerans was involved, higher lactic acid concentrations appeared, while all fermentations where Schizosaccharomyces pombe was involved, lower levels in malic acid concentration took place.
30712100	5	30	theme	mannose	779:785	arg1	concentration					751:763	the highest concentration	739:763	the highest concentration of hydrolyzed mannose	739:785	Fermentations with the highest concentration of hydrolyzed mannose showed the highest mouthfeel properties, but the lack of acidity reduced their overall impression.
30712100	2	31	theme	lactic	401:406	arg1	concentrations					413:426	higher lactic acid concentrations	394:426	higher lactic acid concentrations	394:426	In all fermentations where Lachancea thermotolerans was involved, higher lactic acid concentrations appeared, while all fermentations where Schizosaccharomyces pombe was involved, lower levels in malic acid concentration took place.
30712100	2	32	theme	malic	524:528	arg1	concentration					535:547	malic acid concentration	524:547	malic acid concentration	524:547	In all fermentations where Lachancea thermotolerans was involved, higher lactic acid concentrations appeared, while all fermentations where Schizosaccharomyces pombe was involved, lower levels in malic acid concentration took place.
30712100	6	33	theme	pombe	944:948	arg1	combination					904:914	a combination	902:914	a combination of L. thermotolerans and S. pombe	902:948	Wines made from a combination of L. thermotolerans and S. pombe showed the highest overall impression and were preferred by the tasters due to the balance between mouthfeel properties and acidity.
30712100	2	34	theme	higher	394:399	arg1	concentrations					413:426	higher lactic acid concentrations	394:426	higher lactic acid concentrations	394:426	In all fermentations where Lachancea thermotolerans was involved, higher lactic acid concentrations appeared, while all fermentations where Schizosaccharomyces pombe was involved, lower levels in malic acid concentration took place.
30712100	1	35	from	cooperation	245:255	arg1	production					308:317	the production	304:317	the production of wine	304:325	This study researched the winemaking performance of new biotechnology involving the cooperation of Lachancea and Schizosaccharomyces genera in the production of wine.
30712100	4	36	theme	different	689:697	arg1	trials					712:717	the different fermentation trials	685:717	the different fermentation trials	685:717	Differences in mouthfeel properties and acidity occurred in the different fermentation trials.
30712100	5	37	theme	highest	798:804	arg1	properties					816:825	the highest mouthfeel properties	794:825	the highest mouthfeel properties	794:825	Fermentations with the highest concentration of hydrolyzed mannose showed the highest mouthfeel properties, but the lack of acidity reduced their overall impression.
30712100	5	38	with	Fermentations	720:732	arg1	concentration					751:763	the highest concentration	739:763	the highest concentration of hydrolyzed mannose	739:785	Fermentations with the highest concentration of hydrolyzed mannose showed the highest mouthfeel properties, but the lack of acidity reduced their overall impression.
30712100	1	39	theme	Schizosaccharomyces	274:292	arg1	genera					294:299	Schizosaccharomyces genera	274:299	Schizosaccharomyces genera	274:299	This study researched the winemaking performance of new biotechnology involving the cooperation of Lachancea and Schizosaccharomyces genera in the production of wine.
30712100	6	40	theme	thermotolerans	922:935	arg1	combination					904:914	a combination	902:914	a combination of L. thermotolerans and S. pombe	902:948	Wines made from a combination of L. thermotolerans and S. pombe showed the highest overall impression and were preferred by the tasters due to the balance between mouthfeel properties and acidity.
30712100	1	41	theme	genera	294:299	arg1	cooperation					245:255	the cooperation	241:255	the cooperation of Lachancea and Schizosaccharomyces genera in the production of wine	241:325	This study researched the winemaking performance of new biotechnology involving the cooperation of Lachancea and Schizosaccharomyces genera in the production of wine.
30712100	0	42	theme	thermotolerans	72:85	arg1	fermentation					16:27	Mixed alcoholic fermentation	0:27	Mixed alcoholic fermentation of Schizosaccharomyces pombe and Lachancea thermotolerans	0:85	Mixed alcoholic fermentation of Schizosaccharomyces pombe and Lachancea thermotolerans and its influence on mannose-containing polysaccharides wine Composition.
30712100	0	42	theme	thermotolerans	72:85	arg1	influence					95:103	its influence	91:103	its influence on mannose-containing polysaccharides wine Composition	91:158	Mixed alcoholic fermentation of Schizosaccharomyces pombe and Lachancea thermotolerans and its influence on mannose-containing polysaccharides wine Composition.
30712100	6	43	theme	due	1022:1024	arg1	tasters					1014:1020	the tasters	1010:1020	the tasters due to the balance between mouthfeel properties and acidity	1010:1080	Wines made from a combination of L. thermotolerans and S. pombe showed the highest overall impression and were preferred by the tasters due to the balance between mouthfeel properties and acidity.
30712100	3	44	theme	final	593:597	arg1	wines					599:603	the final wines	589:603	the final wines	589:603	The sensorial properties of the final wines varied accordingly.
30712100	4	45	theme	fermentation	699:710	arg1	trials					712:717	the different fermentation trials	685:717	the different fermentation trials	685:717	Differences in mouthfeel properties and acidity occurred in the different fermentation trials.
30343921	9	0	from	increase	1630:1637	arg1	goats					1653:1657	goats	1653:1657	goats	1653:1657	With COS, this decrease was compensated by an increase of >C16 FA in goats but not in cows, and the >C16 FA yield decreased with MAP in both species.
30343921	9	0	from	increase	1630:1637	arg1	cows					1670:1673	cows	1670:1673	cows	1670:1673	With COS, this decrease was compensated by an increase of >C16 FA in goats but not in cows, and the >C16 FA yield decreased with MAP in both species.
30343921	10	1	theme	FA	1789:1790	arg1	yield					1771:1775	the yield	1767:1775	the yield of milk C16 FA (mmol/d per kilogram of body weight) in cows	1767:1835	Supplementation of HPO increased the yield of milk C16 FA (mmol/d per kilogram of body weight) in cows.
30343921	10	2	from	cows	1832:1835	arg1	yield					1771:1775	the yield	1767:1775	the yield of milk C16 FA (mmol/d per kilogram of body weight) in cows	1767:1835	Supplementation of HPO increased the yield of milk C16 FA (mmol/d per kilogram of body weight) in cows.
30343921	1	3	theme	goat	202:205	arg1	performance					207:217	goat performance	202:217	goat performance	202:217	A direct comparison of cow and goat performance and milk fatty acid (FA) responses to diets that either induce milk fat depression or increase milk fat content in cows suggests species-specific regulation of lipid metabolism, including mammary lipogenesis.
30343921	10	4	theme	milk	1780:1783	arg1	FA					1789:1790	milk C16 FA	1780:1790	milk C16 FA (mmol/d per kilogram of body weight) in cows	1780:1835	Supplementation of HPO increased the yield of milk C16 FA (mmol/d per kilogram of body weight) in cows.
30343921	0	5	from	expression	120:129	arg1	goats					143:147	goats	143:147	goats	143:147	Effect of diets supplemented with starch and corn oil, marine algae, or hydrogenated palm oil on mammary lipogenic gene expression in cows and goats: A comparative study.
30343921	0	5	from	expression	120:129	arg1	cows					134:137	cows	134:137	cows	134:137	Effect of diets supplemented with starch and corn oil, marine algae, or hydrogenated palm oil on mammary lipogenic gene expression in cows and goats: A comparative study.
30343921	1	6	theme	lipid	379:383	arg1	metabolism					385:394	lipid metabolism	379:394	lipid metabolism	379:394	A direct comparison of cow and goat performance and milk fatty acid (FA) responses to diets that either induce milk fat depression or increase milk fat content in cows suggests species-specific regulation of lipid metabolism, including mammary lipogenesis.
30343921	12	7	theme	large	2079:2083	arg1	changes					2085:2091	large changes	2079:2091	large changes	2079:2091	This absence of large changes might be due to post-transcriptional regulation of these genes and related to the time of sampling of the mammary tissue relative to the previous meal and milking or to differences in the availability of substrate for the corresponding proteins.
30343921	0	8	theme	comparative	152:162	arg1	study					164:168	A comparative study	150:168	A comparative study	150:168	Effect of diets supplemented with starch and corn oil, marine algae, or hydrogenated palm oil on mammary lipogenic gene expression in cows and goats: A comparative study.
30343921	6	9	theme	dietary	1271:1277	arg1	treatments					1279:1288	the dietary treatments	1267:1288	the dietary treatments	1267:1288	The results showed significant differences in the milk fat response of cows and goats to the dietary treatments.
30343921	12	10	theme	sampling	2183:2190	arg1	time					2175:2178	the time	2171:2178	the time of sampling of the mammary tissue relative to the previous meal and milking or to differences in the availability of substrate for the corresponding proteins	2171:2336	This absence of large changes might be due to post-transcriptional regulation of these genes and related to the time of sampling of the mammary tissue relative to the previous meal and milking or to differences in the availability of substrate for the corresponding proteins.
30343921	12	11	theme	post-transcriptional	2109:2128	arg1	regulation					2130:2139	post-transcriptional regulation	2109:2139	post-transcriptional regulation of these genes	2109:2154	This absence of large changes might be due to post-transcriptional regulation of these genes and related to the time of sampling of the mammary tissue relative to the previous meal and milking or to differences in the availability of substrate for the corresponding proteins.
30343921	9	12	theme	>C16	1684:1687	arg1	yield					1692:1696	the >C16 FA yield	1680:1696	the >C16 FA yield	1680:1696	With COS, this decrease was compensated by an increase of >C16 FA in goats but not in cows, and the >C16 FA yield decreased with MAP in both species.
30343921	1	13	theme	mammary	407:413	arg1	lipogenesis					415:425	mammary lipogenesis	407:425	mammary lipogenesis	407:425	A direct comparison of cow and goat performance and milk fatty acid (FA) responses to diets that either induce milk fat depression or increase milk fat content in cows suggests species-specific regulation of lipid metabolism, including mammary lipogenesis.
30343921	13	14	dep	FADS3	2507:2511	arg1	ACSL1					2514:2518	ACSL1	2514:2518	ACSL1	2514:2518	However, the abundance of 14 mRNA among the 21 encoding for genes studied in the mammary gland was significantly different among species, with 5 more abundant in cows (FADS3, ACSL1, PPARA, LXRA, and PPARG1) and 10 more abundant in goats (FASN, CD36, FABP3, LPL, GPAM, LPIN1, CSN2, MFGE8, and INSIG1).
30343921	13	14	dep	FADS3	2507:2511	arg1	LXRA					2528:2531	LXRA	2528:2531	LXRA	2528:2531	However, the abundance of 14 mRNA among the 21 encoding for genes studied in the mammary gland was significantly different among species, with 5 more abundant in cows (FADS3, ACSL1, PPARA, LXRA, and PPARG1) and 10 more abundant in goats (FASN, CD36, FABP3, LPL, GPAM, LPIN1, CSN2, MFGE8, and INSIG1).
30343921	13	14	dep	FADS3	2507:2511	arg1	PPARG1					2538:2543	PPARG1	2538:2543	PPARG1	2538:2543	However, the abundance of 14 mRNA among the 21 encoding for genes studied in the mammary gland was significantly different among species, with 5 more abundant in cows (FADS3, ACSL1, PPARA, LXRA, and PPARG1) and 10 more abundant in goats (FASN, CD36, FABP3, LPL, GPAM, LPIN1, CSN2, MFGE8, and INSIG1).
30343921	13	14	dep	FADS3	2507:2511	arg1	PPARA					2521:2525	PPARA	2521:2525	PPARA	2521:2525	However, the abundance of 14 mRNA among the 21 encoding for genes studied in the mammary gland was significantly different among species, with 5 more abundant in cows (FADS3, ACSL1, PPARA, LXRA, and PPARG1) and 10 more abundant in goats (FASN, CD36, FABP3, LPL, GPAM, LPIN1, CSN2, MFGE8, and INSIG1).
30343921	3	15	theme	%	848:848	arg1	oil					837:839	hydrogenated palm oil	819:839	hydrogenated palm oil (HPO; 3% DMI)	819:853	Twelve Holstein cows and 12 Alpine goats were fed a basal diet containing no additional lipid (CTL) or a similar diet supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.
30343921	3	15	theme	%	848:848	arg1	DMI					850:852	HPO; 3% DMI	842:852	HPO; 3% DMI	842:852	Twelve Holstein cows and 12 Alpine goats were fed a basal diet containing no additional lipid (CTL) or a similar diet supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.
30343921	13	16	dep	cows	2501:2504	arg1	FADS3					2507:2511	FADS3	2507:2511	FADS3	2507:2511	However, the abundance of 14 mRNA among the 21 encoding for genes studied in the mammary gland was significantly different among species, with 5 more abundant in cows (FADS3, ACSL1, PPARA, LXRA, and PPARG1) and 10 more abundant in goats (FASN, CD36, FABP3, LPL, GPAM, LPIN1, CSN2, MFGE8, and INSIG1).
30343921	3	17	with	design	890:895	arg1	periods					920:926	28-d experimental periods	902:926	28-d experimental periods	902:926	Twelve Holstein cows and 12 Alpine goats were fed a basal diet containing no additional lipid (CTL) or a similar diet supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.
30343921	2	18	theme	responsible	492:502	arg1	mechanisms					481:490	potential mechanisms	471:490	potential mechanisms responsible for the differences in mammary lipogenesis due to diet and ruminant species	471:578	This experiment was conducted to highlight potential mechanisms responsible for the differences in mammary lipogenesis due to diet and ruminant species.
30343921	14	19	theme	lipid	2679:2683	arg1	metabolism					2685:2694	mammary lipid metabolism	2671:2694	mammary lipid metabolism	2671:2694	These species specificities of mammary lipid metabolism require further investigation.
30343921	0	20	theme	mammary	97:103	arg1	expression					120:129	mammary lipogenic gene expression	97:129	mammary lipogenic gene expression in cows and goats	97:147	Effect of diets supplemented with starch and corn oil, marine algae, or hydrogenated palm oil on mammary lipogenic gene expression in cows and goats: A comparative study.
30343921	1	21	theme	fat	319:321	arg1	content					323:329	milk fat content	314:329	milk fat content in cows	314:337	A direct comparison of cow and goat performance and milk fatty acid (FA) responses to diets that either induce milk fat depression or increase milk fat content in cows suggests species-specific regulation of lipid metabolism, including mammary lipogenesis.
30343921	10	22	dep	FA	1789:1790	arg1	mmol/d					1793:1798	mmol/d	1793:1798	mmol/d	1793:1798	Supplementation of HPO increased the yield of milk C16 FA (mmol/d per kilogram of body weight) in cows.
30343921	3	23	theme	similar	686:692	arg1	diet					694:697	a similar diet	684:697	a similar diet supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI)	684:853	Twelve Holstein cows and 12 Alpine goats were fed a basal diet containing no additional lipid (CTL) or a similar diet supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.
30343921	0	24	theme	gene	115:118	arg1	expression					120:129	mammary lipogenic gene expression	97:129	mammary lipogenic gene expression in cows and goats	97:147	Effect of diets supplemented with starch and corn oil, marine algae, or hydrogenated palm oil on mammary lipogenic gene expression in cows and goats: A comparative study.
30343921	12	25	theme	tissue	2207:2212	arg1	sampling					2183:2190	sampling	2183:2190	sampling of the mammary tissue relative to the previous meal and milking or to differences in the availability of substrate for the corresponding proteins	2183:2336	This absence of large changes might be due to post-transcriptional regulation of these genes and related to the time of sampling of the mammary tissue relative to the previous meal and milking or to differences in the availability of substrate for the corresponding proteins.
30343921	14	26	theme	species	2646:2652	arg1	specificities					2654:2666	These species specificities	2640:2666	These species specificities of mammary lipid metabolism	2640:2694	These species specificities of mammary lipid metabolism require further investigation.
30343921	3	27	theme	[5	726:727	arg1	DMI					749:751	DMI	749:751	DMI	749:751	Twelve Holstein cows and 12 Alpine goats were fed a basal diet containing no additional lipid (CTL) or a similar diet supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.
30343921	3	27	theme	[5	726:727	arg1	intake					741:746	corn oil [5% dry matter intake	717:746	corn oil [5% dry matter intake (DMI)]	717:753	Twelve Holstein cows and 12 Alpine goats were fed a basal diet containing no additional lipid (CTL) or a similar diet supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.
30343921	8	28	theme	weight	1556:1561	arg1	kilogram					1539:1546	kilogram	1539:1546	kilogram of body weight	1539:1561	In both species, COS and MAP lowered the yields (mmol/d per kilogram of body weight) of <C16 and C16 FA.
30343921	11	29	theme	transcription	2016:2028	arg1	INSIG1					2046:2051	INSIG1	2046:2051	INSIG1	2046:2051	These variations in milk fat content and FA secretion were not associated with modifications in the mammary expression of 21 genes involved in major lipid pathways, except for 3 transcription factors: PPARA, INSIG1, and SP1.
30343921	11	29	theme	transcription	2016:2028	arg1	SP1					2058:2060	SP1	2058:2060	SP1	2058:2060	These variations in milk fat content and FA secretion were not associated with modifications in the mammary expression of 21 genes involved in major lipid pathways, except for 3 transcription factors: PPARA, INSIG1, and SP1.
30343921	11	29	theme	transcription	2016:2028	arg1	PPARA					2039:2043	PPARA	2039:2043	PPARA	2039:2043	These variations in milk fat content and FA secretion were not associated with modifications in the mammary expression of 21 genes involved in major lipid pathways, except for 3 transcription factors: PPARA, INSIG1, and SP1.
30343921	11	29	theme	transcription	2016:2028	arg1	factors					2030:2036	3 transcription factors	2014:2036	3 transcription factors: PPARA, INSIG1, and SP1	2014:2060	These variations in milk fat content and FA secretion were not associated with modifications in the mammary expression of 21 genes involved in major lipid pathways, except for 3 transcription factors: PPARA, INSIG1, and SP1.
30343921	12	30	theme	substrate	2297:2305	arg1	availability					2281:2292	the availability	2277:2292	the availability of substrate for the corresponding proteins	2277:2336	This absence of large changes might be due to post-transcriptional regulation of these genes and related to the time of sampling of the mammary tissue relative to the previous meal and milking or to differences in the availability of substrate for the corresponding proteins.
30343921	11	31	theme	mammary	1938:1944	arg1	expression					1946:1955	the mammary expression	1934:1955	the mammary expression of 21 genes involved in major lipid pathways	1934:2000	These variations in milk fat content and FA secretion were not associated with modifications in the mammary expression of 21 genes involved in major lipid pathways, except for 3 transcription factors: PPARA, INSIG1, and SP1.
30343921	3	32	theme	corn	717:720	arg1	DMI					749:751	DMI	749:751	DMI	749:751	Twelve Holstein cows and 12 Alpine goats were fed a basal diet containing no additional lipid (CTL) or a similar diet supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.
30343921	3	32	theme	corn	717:720	arg1	intake					741:746	corn oil [5% dry matter intake	717:746	corn oil [5% dry matter intake (DMI)]	717:753	Twelve Holstein cows and 12 Alpine goats were fed a basal diet containing no additional lipid (CTL) or a similar diet supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.
30343921	14	33	theme	further	2704:2710	arg1	investigation					2712:2724	further investigation	2704:2724	further investigation	2704:2724	These species specificities of mammary lipid metabolism require further investigation.
30343921	11	34	theme	genes	1963:1967	arg1	expression					1946:1955	the mammary expression	1934:1955	the mammary expression of 21 genes involved in major lipid pathways	1934:2000	These variations in milk fat content and FA secretion were not associated with modifications in the mammary expression of 21 genes involved in major lipid pathways, except for 3 transcription factors: PPARA, INSIG1, and SP1.
30343921	4	35	theme	milk	941:944	arg1	composition					946:956	milk composition	941:956	milk composition	941:956	Milk yield, milk composition, FA profile, and secretions were measured.
30343921	3	36	contain	containing	644:653	arg1	diet					639:642	a basal diet	631:642	a basal diet containing no additional lipid (CTL)	631:679	Twelve Holstein cows and 12 Alpine goats were fed a basal diet containing no additional lipid (CTL) or a similar diet supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.
30343921	3	36	contain	containing	644:653	arg2	CTL					676:678	CTL	676:678	CTL	676:678	Twelve Holstein cows and 12 Alpine goats were fed a basal diet containing no additional lipid (CTL) or a similar diet supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.
30343921	3	36	contain	containing	644:653	arg2	lipid					669:673	no additional lipid	655:673	no additional lipid (CTL)	655:679	Twelve Holstein cows and 12 Alpine goats were fed a basal diet containing no additional lipid (CTL) or a similar diet supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.
30343921	0	37	theme	marine	55:60	arg1	algae					62:66	marine algae	55:66	marine algae	55:66	Effect of diets supplemented with starch and corn oil, marine algae, or hydrogenated palm oil on mammary lipogenic gene expression in cows and goats: A comparative study.
30343921	3	38	theme	wheat	759:763	arg1	starch					765:770	wheat starch	759:770	wheat starch (COS)	759:776	Twelve Holstein cows and 12 Alpine goats were fed a basal diet containing no additional lipid (CTL) or a similar diet supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.
30343921	3	38	theme	wheat	759:763	arg1	COS					773:775	COS	773:775	COS	773:775	Twelve Holstein cows and 12 Alpine goats were fed a basal diet containing no additional lipid (CTL) or a similar diet supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.
30343921	7	39	theme	fat	1300:1302	arg1	content					1304:1310	fat content	1300:1310	fat content	1300:1310	In cows, fat content was lowered by COS (-45%) and MAP (-22%) and increased by HPO (+13%) compared with CTL, and in goats only MAP had an effect compared with CTL, with a decrease of 15%.
30343921	2	40	theme	potential	471:479	arg1	mechanisms					481:490	potential mechanisms	471:490	potential mechanisms responsible for the differences in mammary lipogenesis due to diet and ruminant species	471:578	This experiment was conducted to highlight potential mechanisms responsible for the differences in mammary lipogenesis due to diet and ruminant species.
30343921	0	41	from	Effect	0:5	arg1	expression					120:129	mammary lipogenic gene expression	97:129	mammary lipogenic gene expression in cows and goats	97:147	Effect of diets supplemented with starch and corn oil, marine algae, or hydrogenated palm oil on mammary lipogenic gene expression in cows and goats: A comparative study.
30343921	11	42	theme	fat	1863:1865	arg1	content					1867:1873	milk fat content	1858:1873	milk fat content	1858:1873	These variations in milk fat content and FA secretion were not associated with modifications in the mammary expression of 21 genes involved in major lipid pathways, except for 3 transcription factors: PPARA, INSIG1, and SP1.
30343921	5	43	theme	period	1030:1035	arg1	d					1004:1004	d 27	1004:1007	d 27 of each experimental period	1004:1035	On d 27 of each experimental period, the mRNA abundance of 21 genes involved in lipid metabolism or enzyme activities or both were measured in mammary tissue sampled by biopsy.
30343921	3	44	theme	dry	730:732	arg1	DMI					749:751	DMI	749:751	DMI	749:751	Twelve Holstein cows and 12 Alpine goats were fed a basal diet containing no additional lipid (CTL) or a similar diet supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.
30343921	3	44	theme	dry	730:732	arg1	intake					741:746	corn oil [5% dry matter intake	717:746	corn oil [5% dry matter intake (DMI)]	717:753	Twelve Holstein cows and 12 Alpine goats were fed a basal diet containing no additional lipid (CTL) or a similar diet supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.
30343921	1	45	from	content	323:329	arg1	cows					334:337	cows	334:337	cows	334:337	A direct comparison of cow and goat performance and milk fatty acid (FA) responses to diets that either induce milk fat depression or increase milk fat content in cows suggests species-specific regulation of lipid metabolism, including mammary lipogenesis.
30343921	1	46	theme	milk	282:285	arg1	fat					287:289	milk fat	282:289	milk fat depression	282:300	A direct comparison of cow and goat performance and milk fatty acid (FA) responses to diets that either induce milk fat depression or increase milk fat content in cows suggests species-specific regulation of lipid metabolism, including mammary lipogenesis.
30343921	3	47	theme	marine	779:784	arg1	DMI					810:812	MAP; 1.5% DMI	800:812	MAP; 1.5% DMI	800:812	Twelve Holstein cows and 12 Alpine goats were fed a basal diet containing no additional lipid (CTL) or a similar diet supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.
30343921	3	47	theme	marine	779:784	arg1	powder					792:797	marine algae powder	779:797	marine algae powder (MAP; 1.5% DMI)	779:813	Twelve Holstein cows and 12 Alpine goats were fed a basal diet containing no additional lipid (CTL) or a similar diet supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.
30343921	11	48	theme	lipid	1987:1991	arg1	pathways					1993:2000	major lipid pathways	1981:2000	major lipid pathways	1981:2000	These variations in milk fat content and FA secretion were not associated with modifications in the mammary expression of 21 genes involved in major lipid pathways, except for 3 transcription factors: PPARA, INSIG1, and SP1.
30343921	13	49	dep	goats	2570:2574	arg1	FASN					2577:2580	FASN	2577:2580	FASN	2577:2580	However, the abundance of 14 mRNA among the 21 encoding for genes studied in the mammary gland was significantly different among species, with 5 more abundant in cows (FADS3, ACSL1, PPARA, LXRA, and PPARG1) and 10 more abundant in goats (FASN, CD36, FABP3, LPL, GPAM, LPIN1, CSN2, MFGE8, and INSIG1).
30343921	5	50	theme	lipid	1081:1085	arg1	metabolism					1087:1096	lipid metabolism	1081:1096	lipid metabolism	1081:1096	On d 27 of each experimental period, the mRNA abundance of 21 genes involved in lipid metabolism or enzyme activities or both were measured in mammary tissue sampled by biopsy.
30343921	11	51	from	modifications	1917:1929	arg1	expression					1946:1955	the mammary expression	1934:1955	the mammary expression of 21 genes involved in major lipid pathways	1934:2000	These variations in milk fat content and FA secretion were not associated with modifications in the mammary expression of 21 genes involved in major lipid pathways, except for 3 transcription factors: PPARA, INSIG1, and SP1.
30343921	0	52	theme	hydrogenated	72:83	arg1	oil					90:92	hydrogenated palm oil	72:92	hydrogenated palm oil	72:92	Effect of diets supplemented with starch and corn oil, marine algae, or hydrogenated palm oil on mammary lipogenic gene expression in cows and goats: A comparative study.
30343921	12	53	from	differences	2262:2272	arg1	availability					2281:2292	the availability	2277:2292	the availability of substrate for the corresponding proteins	2277:2336	This absence of large changes might be due to post-transcriptional regulation of these genes and related to the time of sampling of the mammary tissue relative to the previous meal and milking or to differences in the availability of substrate for the corresponding proteins.
30343921	3	54	theme	Latin	877:881	arg1	design					890:895	a 4 × 4 Latin square design	869:895	a 4 × 4 Latin square design with 28-d experimental periods	869:926	Twelve Holstein cows and 12 Alpine goats were fed a basal diet containing no additional lipid (CTL) or a similar diet supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.
30343921	9	55	from	FA	1647:1648	arg1	goats					1653:1657	goats	1653:1657	goats	1653:1657	With COS, this decrease was compensated by an increase of >C16 FA in goats but not in cows, and the >C16 FA yield decreased with MAP in both species.
30343921	9	55	from	FA	1647:1648	arg1	cows					1670:1673	cows	1670:1673	cows	1670:1673	With COS, this decrease was compensated by an increase of >C16 FA in goats but not in cows, and the >C16 FA yield decreased with MAP in both species.
30343921	8	56	theme	FA	1580:1581	arg1	yields					1520:1525	the yields	1516:1525	the yields (mmol/d per kilogram of body weight) of <C16 and C16 FA	1516:1581	In both species, COS and MAP lowered the yields (mmol/d per kilogram of body weight) of <C16 and C16 FA.
30343921	1	57	theme	milk	223:226	arg1	acid					234:237	milk fatty acid	223:237	milk fatty acid (FA)	223:242	A direct comparison of cow and goat performance and milk fatty acid (FA) responses to diets that either induce milk fat depression or increase milk fat content in cows suggests species-specific regulation of lipid metabolism, including mammary lipogenesis.
30343921	1	57	theme	milk	223:226	arg1	FA					240:241	FA	240:241	FA	240:241	A direct comparison of cow and goat performance and milk fatty acid (FA) responses to diets that either induce milk fat depression or increase milk fat content in cows suggests species-specific regulation of lipid metabolism, including mammary lipogenesis.
30343921	3	58	theme	×	873:873	arg1	design					890:895	a 4 × 4 Latin square design	869:895	a 4 × 4 Latin square design with 28-d experimental periods	869:926	Twelve Holstein cows and 12 Alpine goats were fed a basal diet containing no additional lipid (CTL) or a similar diet supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.
30343921	3	59	theme	MAP	800:802	arg1	DMI					810:812	MAP; 1.5% DMI	800:812	MAP; 1.5% DMI	800:812	Twelve Holstein cows and 12 Alpine goats were fed a basal diet containing no additional lipid (CTL) or a similar diet supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.
30343921	3	59	theme	MAP	800:802	arg1	powder					792:797	marine algae powder	779:797	marine algae powder (MAP; 1.5% DMI)	779:813	Twelve Holstein cows and 12 Alpine goats were fed a basal diet containing no additional lipid (CTL) or a similar diet supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.
30343921	10	60	theme	HPO	1753:1755	arg1	Supplementation					1734:1748	Supplementation	1734:1748	Supplementation of HPO	1734:1755	Supplementation of HPO increased the yield of milk C16 FA (mmol/d per kilogram of body weight) in cows.
30343921	0	61	theme	diets	10:14	arg1	Effect					0:5	Effect	0:5	Effect of diets supplemented with starch and corn oil, marine algae, or hydrogenated palm oil on mammary lipogenic gene expression in cows and goats	0:147	Effect of diets supplemented with starch and corn oil, marine algae, or hydrogenated palm oil on mammary lipogenic gene expression in cows and goats: A comparative study.
30343921	1	62	theme	acid	234:237	arg1	responses					244:252	goat performance and milk fatty acid (FA) responses	202:252	goat performance and milk fatty acid (FA) responses to diets that either induce milk fat depression or increase milk fat content in cows	202:337	A direct comparison of cow and goat performance and milk fatty acid (FA) responses to diets that either induce milk fat depression or increase milk fat content in cows suggests species-specific regulation of lipid metabolism, including mammary lipogenesis.
30343921	3	63	theme	28-d	902:905	arg1	periods					920:926	28-d experimental periods	902:926	28-d experimental periods	902:926	Twelve Holstein cows and 12 Alpine goats were fed a basal diet containing no additional lipid (CTL) or a similar diet supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.
30343921	8	64	dep	yields	1520:1525	arg1	mmol/d					1528:1533	mmol/d	1528:1533	mmol/d	1528:1533	In both species, COS and MAP lowered the yields (mmol/d per kilogram of body weight) of <C16 and C16 FA.
30343921	12	65	theme	relative	2214:2221	arg1	tissue					2207:2212	the mammary tissue	2195:2212	the mammary tissue relative to the previous meal and milking or to differences in the availability of substrate for the corresponding proteins	2195:2336	This absence of large changes might be due to post-transcriptional regulation of these genes and related to the time of sampling of the mammary tissue relative to the previous meal and milking or to differences in the availability of substrate for the corresponding proteins.
30343921	12	66	theme	previous	2230:2237	arg1	meal					2239:2242	the previous meal	2226:2242	the previous meal	2226:2242	This absence of large changes might be due to post-transcriptional regulation of these genes and related to the time of sampling of the mammary tissue relative to the previous meal and milking or to differences in the availability of substrate for the corresponding proteins.
30343921	1	67	theme	responses	244:252	arg1	comparison					180:189	A direct comparison	171:189	A direct comparison of cow and goat performance and milk fatty acid (FA) responses to diets that either induce milk fat depression or increase milk fat content in cows	171:337	A direct comparison of cow and goat performance and milk fatty acid (FA) responses to diets that either induce milk fat depression or increase milk fat content in cows suggests species-specific regulation of lipid metabolism, including mammary lipogenesis.
30343921	13	68	from	abundant	2489:2496	arg1	cows					2501:2504	cows	2501:2504	cows (FADS3, ACSL1, PPARA, LXRA, and PPARG1)	2501:2544	However, the abundance of 14 mRNA among the 21 encoding for genes studied in the mammary gland was significantly different among species, with 5 more abundant in cows (FADS3, ACSL1, PPARA, LXRA, and PPARG1) and 10 more abundant in goats (FASN, CD36, FABP3, LPL, GPAM, LPIN1, CSN2, MFGE8, and INSIG1).
30343921	9	69	theme	>C16	1642:1645	arg1	FA					1647:1648	>C16 FA	1642:1648	>C16 FA in goats but not in cows	1642:1673	With COS, this decrease was compensated by an increase of >C16 FA in goats but not in cows, and the >C16 FA yield decreased with MAP in both species.
30343921	5	70	theme	mammary	1144:1150	arg1	tissue					1152:1157	mammary tissue	1144:1157	mammary tissue sampled by biopsy	1144:1175	On d 27 of each experimental period, the mRNA abundance of 21 genes involved in lipid metabolism or enzyme activities or both were measured in mammary tissue sampled by biopsy.
30343921	1	71	theme	direct	173:178	arg1	comparison					180:189	A direct comparison	171:189	A direct comparison of cow and goat performance and milk fatty acid (FA) responses to diets that either induce milk fat depression or increase milk fat content in cows	171:337	A direct comparison of cow and goat performance and milk fatty acid (FA) responses to diets that either induce milk fat depression or increase milk fat content in cows suggests species-specific regulation of lipid metabolism, including mammary lipogenesis.
30343921	13	72	theme	mRNA	2368:2371	arg1	different					2452:2460	different	2452:2460	different	2452:2460	However, the abundance of 14 mRNA among the 21 encoding for genes studied in the mammary gland was significantly different among species, with 5 more abundant in cows (FADS3, ACSL1, PPARA, LXRA, and PPARG1) and 10 more abundant in goats (FASN, CD36, FABP3, LPL, GPAM, LPIN1, CSN2, MFGE8, and INSIG1).
30343921	13	72	theme	mRNA	2368:2371	arg1	abundance					2352:2360	the abundance	2348:2360	the abundance of 14 mRNA	2348:2371	However, the abundance of 14 mRNA among the 21 encoding for genes studied in the mammary gland was significantly different among species, with 5 more abundant in cows (FADS3, ACSL1, PPARA, LXRA, and PPARG1) and 10 more abundant in goats (FASN, CD36, FABP3, LPL, GPAM, LPIN1, CSN2, MFGE8, and INSIG1).
30343921	3	73	theme	hydrogenated	819:830	arg1	oil					837:839	hydrogenated palm oil	819:839	hydrogenated palm oil (HPO; 3% DMI)	819:853	Twelve Holstein cows and 12 Alpine goats were fed a basal diet containing no additional lipid (CTL) or a similar diet supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.
30343921	3	73	theme	hydrogenated	819:830	arg1	DMI					850:852	HPO; 3% DMI	842:852	HPO; 3% DMI	842:852	Twelve Holstein cows and 12 Alpine goats were fed a basal diet containing no additional lipid (CTL) or a similar diet supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.
30343921	11	74	from	variations	1844:1853	arg1	content					1867:1873	milk fat content	1858:1873	milk fat content	1858:1873	These variations in milk fat content and FA secretion were not associated with modifications in the mammary expression of 21 genes involved in major lipid pathways, except for 3 transcription factors: PPARA, INSIG1, and SP1.
30343921	11	74	from	variations	1844:1853	arg1	secretion					1882:1890	FA secretion	1879:1890	FA secretion	1879:1890	These variations in milk fat content and FA secretion were not associated with modifications in the mammary expression of 21 genes involved in major lipid pathways, except for 3 transcription factors: PPARA, INSIG1, and SP1.
30343921	6	75	theme	milk	1228:1231	arg1	response					1237:1244	the milk fat response	1224:1244	the milk fat response of cows and goats to the dietary treatments	1224:1288	The results showed significant differences in the milk fat response of cows and goats to the dietary treatments.
30343921	9	76	from	goats	1653:1657	arg1	increase					1630:1637	an increase	1627:1637	an increase of >C16 FA in goats but not in cows	1627:1673	With COS, this decrease was compensated by an increase of >C16 FA in goats but not in cows, and the >C16 FA yield decreased with MAP in both species.
30343921	13	77	dep	abundant	2489:2496	arg1	5					2482:2482	5	2482:2482	5	2482:2482	However, the abundance of 14 mRNA among the 21 encoding for genes studied in the mammary gland was significantly different among species, with 5 more abundant in cows (FADS3, ACSL1, PPARA, LXRA, and PPARG1) and 10 more abundant in goats (FASN, CD36, FABP3, LPL, GPAM, LPIN1, CSN2, MFGE8, and INSIG1).
30343921	5	78	theme	both	1122:1125	arg1	abundance					1047:1055	the mRNA abundance	1038:1055	the mRNA abundance of 21 genes involved in lipid metabolism or enzyme activities or both	1038:1125	On d 27 of each experimental period, the mRNA abundance of 21 genes involved in lipid metabolism or enzyme activities or both were measured in mammary tissue sampled by biopsy.
30343921	10	79	theme	C16	1785:1787	arg1	FA					1789:1790	milk C16 FA	1780:1790	milk C16 FA (mmol/d per kilogram of body weight) in cows	1780:1835	Supplementation of HPO increased the yield of milk C16 FA (mmol/d per kilogram of body weight) in cows.
30343921	3	80	theme	HPO	842:844	arg1	oil					837:839	hydrogenated palm oil	819:839	hydrogenated palm oil (HPO; 3% DMI)	819:853	Twelve Holstein cows and 12 Alpine goats were fed a basal diet containing no additional lipid (CTL) or a similar diet supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.
30343921	3	80	theme	HPO	842:844	arg1	DMI					850:852	HPO; 3% DMI	842:852	HPO; 3% DMI	842:852	Twelve Holstein cows and 12 Alpine goats were fed a basal diet containing no additional lipid (CTL) or a similar diet supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.
30343921	2	81	from	differences	512:522	arg1	lipogenesis					535:545	mammary lipogenesis	527:545	mammary lipogenesis due to diet and ruminant species	527:578	This experiment was conducted to highlight potential mechanisms responsible for the differences in mammary lipogenesis due to diet and ruminant species.
30343921	3	82	theme	basal	633:637	arg1	diet					639:642	a basal diet	631:642	a basal diet containing no additional lipid (CTL)	631:679	Twelve Holstein cows and 12 Alpine goats were fed a basal diet containing no additional lipid (CTL) or a similar diet supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.
30343921	6	83	theme	significant	1197:1207	arg1	differences					1209:1219	significant differences	1197:1219	significant differences in the milk fat response of cows and goats to the dietary treatments	1197:1288	The results showed significant differences in the milk fat response of cows and goats to the dietary treatments.
30343921	1	84	theme	performance	207:217	arg1	responses					244:252	goat performance and milk fatty acid (FA) responses	202:252	goat performance and milk fatty acid (FA) responses to diets that either induce milk fat depression or increase milk fat content in cows	202:337	A direct comparison of cow and goat performance and milk fatty acid (FA) responses to diets that either induce milk fat depression or increase milk fat content in cows suggests species-specific regulation of lipid metabolism, including mammary lipogenesis.
30343921	12	85	theme	mammary	2199:2205	arg1	tissue					2207:2212	the mammary tissue	2195:2212	the mammary tissue relative to the previous meal and milking or to differences in the availability of substrate for the corresponding proteins	2195:2336	This absence of large changes might be due to post-transcriptional regulation of these genes and related to the time of sampling of the mammary tissue relative to the previous meal and milking or to differences in the availability of substrate for the corresponding proteins.
30343921	10	86	from	yield	1771:1775	arg1	cows					1832:1835	cows	1832:1835	cows	1832:1835	Supplementation of HPO increased the yield of milk C16 FA (mmol/d per kilogram of body weight) in cows.
30343921	1	87	theme	metabolism	385:394	arg1	regulation					365:374	species-specific regulation	348:374	species-specific regulation of lipid metabolism	348:394	A direct comparison of cow and goat performance and milk fatty acid (FA) responses to diets that either induce milk fat depression or increase milk fat content in cows suggests species-specific regulation of lipid metabolism, including mammary lipogenesis.
30343921	13	88	from	goats	2570:2574	arg1	abundant					2558:2565	abundant	2558:2565	abundant	2558:2565	However, the abundance of 14 mRNA among the 21 encoding for genes studied in the mammary gland was significantly different among species, with 5 more abundant in cows (FADS3, ACSL1, PPARA, LXRA, and PPARG1) and 10 more abundant in goats (FASN, CD36, FABP3, LPL, GPAM, LPIN1, CSN2, MFGE8, and INSIG1).
30343921	4	89	theme	Milk	929:932	arg1	yield					934:938	Milk yield	929:938	Milk yield	929:938	Milk yield, milk composition, FA profile, and secretions were measured.
30343921	3	90	theme	additional	658:667	arg1	CTL					676:678	CTL	676:678	CTL	676:678	Twelve Holstein cows and 12 Alpine goats were fed a basal diet containing no additional lipid (CTL) or a similar diet supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.
30343921	3	90	theme	additional	658:667	arg1	lipid					669:673	no additional lipid	655:673	no additional lipid (CTL)	655:679	Twelve Holstein cows and 12 Alpine goats were fed a basal diet containing no additional lipid (CTL) or a similar diet supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.
30343921	13	91	from	abundant	2558:2565	arg1	goats					2570:2574	goats	2570:2574	goats (FASN, CD36, FABP3, LPL, GPAM, LPIN1, CSN2, MFGE8, and INSIG1)	2570:2637	However, the abundance of 14 mRNA among the 21 encoding for genes studied in the mammary gland was significantly different among species, with 5 more abundant in cows (FADS3, ACSL1, PPARA, LXRA, and PPARG1) and 10 more abundant in goats (FASN, CD36, FABP3, LPL, GPAM, LPIN1, CSN2, MFGE8, and INSIG1).
30343921	0	92	dep	study	164:168	arg1	Effect					0:5	Effect	0:5	Effect of diets supplemented with starch and corn oil, marine algae, or hydrogenated palm oil on mammary lipogenic gene expression in cows and goats	0:147	Effect of diets supplemented with starch and corn oil, marine algae, or hydrogenated palm oil on mammary lipogenic gene expression in cows and goats: A comparative study.
30343921	2	93	theme	ruminant	563:570	arg1	species					572:578	ruminant species	563:578	ruminant species	563:578	This experiment was conducted to highlight potential mechanisms responsible for the differences in mammary lipogenesis due to diet and ruminant species.
30343921	9	94	theme	FA	1689:1690	arg1	yield					1692:1696	the >C16 FA yield	1680:1696	the >C16 FA yield	1680:1696	With COS, this decrease was compensated by an increase of >C16 FA in goats but not in cows, and the >C16 FA yield decreased with MAP in both species.
30343921	6	95	theme	goats	1258:1262	arg1	response					1237:1244	the milk fat response	1224:1244	the milk fat response of cows and goats to the dietary treatments	1224:1288	The results showed significant differences in the milk fat response of cows and goats to the dietary treatments.
30343921	10	96	theme	weight	1821:1826	arg1	kilogram					1804:1811	kilogram	1804:1811	kilogram of body weight	1804:1826	Supplementation of HPO increased the yield of milk C16 FA (mmol/d per kilogram of body weight) in cows.
30343921	14	97	theme	metabolism	2685:2694	arg1	specificities					2654:2666	These species specificities	2640:2666	These species specificities of mammary lipid metabolism	2640:2694	These species specificities of mammary lipid metabolism require further investigation.
30343921	0	98	theme	lipogenic	105:113	arg1	expression					120:129	mammary lipogenic gene expression	97:129	mammary lipogenic gene expression in cows and goats	97:147	Effect of diets supplemented with starch and corn oil, marine algae, or hydrogenated palm oil on mammary lipogenic gene expression in cows and goats: A comparative study.
30343921	1	99	theme	milk	314:317	arg1	content					323:329	milk fat content	314:329	milk fat content in cows	314:337	A direct comparison of cow and goat performance and milk fatty acid (FA) responses to diets that either induce milk fat depression or increase milk fat content in cows suggests species-specific regulation of lipid metabolism, including mammary lipogenesis.
30343921	6	100	theme	cows	1249:1252	arg1	response					1237:1244	the milk fat response	1224:1244	the milk fat response of cows and goats to the dietary treatments	1224:1288	The results showed significant differences in the milk fat response of cows and goats to the dietary treatments.
30343921	14	101	theme	mammary	2671:2677	arg1	metabolism					2685:2694	mammary lipid metabolism	2671:2694	mammary lipid metabolism	2671:2694	These species specificities of mammary lipid metabolism require further investigation.
30343921	4	102	theme	FA	959:960	arg1	profile					962:968	FA profile	959:968	FA profile	959:968	Milk yield, milk composition, FA profile, and secretions were measured.
30343921	7	103	theme	%	1476:1476	arg1	decrease					1462:1469	a decrease	1460:1469	a decrease of 15%	1460:1476	In cows, fat content was lowered by COS (-45%) and MAP (-22%) and increased by HPO (+13%) compared with CTL, and in goats only MAP had an effect compared with CTL, with a decrease of 15%.
30343921	2	104	theme	mammary	527:533	arg1	lipogenesis					535:545	mammary lipogenesis	527:545	mammary lipogenesis due to diet and ruminant species	527:578	This experiment was conducted to highlight potential mechanisms responsible for the differences in mammary lipogenesis due to diet and ruminant species.
30343921	13	105	theme	mammary	2420:2426	arg1	gland					2428:2432	the mammary gland	2416:2432	the mammary gland	2416:2432	However, the abundance of 14 mRNA among the 21 encoding for genes studied in the mammary gland was significantly different among species, with 5 more abundant in cows (FADS3, ACSL1, PPARA, LXRA, and PPARG1) and 10 more abundant in goats (FASN, CD36, FABP3, LPL, GPAM, LPIN1, CSN2, MFGE8, and INSIG1).
30343921	5	106	theme	mRNA	1042:1045	arg1	abundance					1047:1055	the mRNA abundance	1038:1055	the mRNA abundance of 21 genes involved in lipid metabolism or enzyme activities or both	1038:1125	On d 27 of each experimental period, the mRNA abundance of 21 genes involved in lipid metabolism or enzyme activities or both were measured in mammary tissue sampled by biopsy.
30343921	3	107	theme	%	728:728	arg1	DMI					749:751	DMI	749:751	DMI	749:751	Twelve Holstein cows and 12 Alpine goats were fed a basal diet containing no additional lipid (CTL) or a similar diet supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.
30343921	3	107	theme	%	728:728	arg1	intake					741:746	corn oil [5% dry matter intake	717:746	corn oil [5% dry matter intake (DMI)]	717:753	Twelve Holstein cows and 12 Alpine goats were fed a basal diet containing no additional lipid (CTL) or a similar diet supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.
30343921	8	108	theme	body	1551:1554	arg1	weight					1556:1561	body weight	1551:1561	body weight	1551:1561	In both species, COS and MAP lowered the yields (mmol/d per kilogram of body weight) of <C16 and C16 FA.
30343921	1	109	theme	species-specific	348:363	arg1	regulation					365:374	species-specific regulation	348:374	species-specific regulation of lipid metabolism	348:394	A direct comparison of cow and goat performance and milk fatty acid (FA) responses to diets that either induce milk fat depression or increase milk fat content in cows suggests species-specific regulation of lipid metabolism, including mammary lipogenesis.
30343921	12	110	theme	changes	2085:2091	arg1	absence					2068:2074	This absence	2063:2074	This absence of large changes	2063:2091	This absence of large changes might be due to post-transcriptional regulation of these genes and related to the time of sampling of the mammary tissue relative to the previous meal and milking or to differences in the availability of substrate for the corresponding proteins.
30343921	3	111	theme	oil	722:724	arg1	DMI					749:751	DMI	749:751	DMI	749:751	Twelve Holstein cows and 12 Alpine goats were fed a basal diet containing no additional lipid (CTL) or a similar diet supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.
30343921	3	111	theme	oil	722:724	arg1	intake					741:746	corn oil [5% dry matter intake	717:746	corn oil [5% dry matter intake (DMI)]	717:753	Twelve Holstein cows and 12 Alpine goats were fed a basal diet containing no additional lipid (CTL) or a similar diet supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.
30343921	12	112	theme	genes	2150:2154	arg1	regulation					2130:2139	post-transcriptional regulation	2109:2139	post-transcriptional regulation of these genes	2109:2154	This absence of large changes might be due to post-transcriptional regulation of these genes and related to the time of sampling of the mammary tissue relative to the previous meal and milking or to differences in the availability of substrate for the corresponding proteins.
30343921	9	113	theme	FA	1647:1648	arg1	increase					1630:1637	an increase	1627:1637	an increase of >C16 FA in goats but not in cows	1627:1673	With COS, this decrease was compensated by an increase of >C16 FA in goats but not in cows, and the >C16 FA yield decreased with MAP in both species.
30343921	11	114	theme	milk	1858:1861	arg1	content					1867:1873	milk fat content	1858:1873	milk fat content	1858:1873	These variations in milk fat content and FA secretion were not associated with modifications in the mammary expression of 21 genes involved in major lipid pathways, except for 3 transcription factors: PPARA, INSIG1, and SP1.
30343921	5	115	theme	experimental	1017:1028	arg1	period					1030:1035	each experimental period	1012:1035	each experimental period	1012:1035	On d 27 of each experimental period, the mRNA abundance of 21 genes involved in lipid metabolism or enzyme activities or both were measured in mammary tissue sampled by biopsy.
30343921	8	116	theme	<C16	1567:1570	arg1	yields					1520:1525	the yields	1516:1525	the yields (mmol/d per kilogram of body weight) of <C16 and C16 FA	1516:1581	In both species, COS and MAP lowered the yields (mmol/d per kilogram of body weight) of <C16 and C16 FA.
30343921	11	117	dep	factors	2030:2036	arg1	INSIG1					2046:2051	INSIG1	2046:2051	INSIG1	2046:2051	These variations in milk fat content and FA secretion were not associated with modifications in the mammary expression of 21 genes involved in major lipid pathways, except for 3 transcription factors: PPARA, INSIG1, and SP1.
30343921	11	117	dep	factors	2030:2036	arg1	SP1					2058:2060	SP1	2058:2060	SP1	2058:2060	These variations in milk fat content and FA secretion were not associated with modifications in the mammary expression of 21 genes involved in major lipid pathways, except for 3 transcription factors: PPARA, INSIG1, and SP1.
30343921	11	117	dep	factors	2030:2036	arg1	PPARA					2039:2043	PPARA	2039:2043	PPARA	2039:2043	These variations in milk fat content and FA secretion were not associated with modifications in the mammary expression of 21 genes involved in major lipid pathways, except for 3 transcription factors: PPARA, INSIG1, and SP1.
30343921	11	117	dep	factors	2030:2036	arg1	factors					2030:2036	3 transcription factors	2014:2036	3 transcription factors: PPARA, INSIG1, and SP1	2014:2060	These variations in milk fat content and FA secretion were not associated with modifications in the mammary expression of 21 genes involved in major lipid pathways, except for 3 transcription factors: PPARA, INSIG1, and SP1.
30343921	11	118	theme	major	1981:1985	arg1	pathways					1993:2000	major lipid pathways	1981:2000	major lipid pathways	1981:2000	These variations in milk fat content and FA secretion were not associated with modifications in the mammary expression of 21 genes involved in major lipid pathways, except for 3 transcription factors: PPARA, INSIG1, and SP1.
30343921	3	119	theme	matter	734:739	arg1	DMI					749:751	DMI	749:751	DMI	749:751	Twelve Holstein cows and 12 Alpine goats were fed a basal diet containing no additional lipid (CTL) or a similar diet supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.
30343921	3	119	theme	matter	734:739	arg1	intake					741:746	corn oil [5% dry matter intake	717:746	corn oil [5% dry matter intake (DMI)]	717:753	Twelve Holstein cows and 12 Alpine goats were fed a basal diet containing no additional lipid (CTL) or a similar diet supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.
30343921	8	120	theme	C16	1576:1578	arg1	FA					1580:1581	C16 FA	1576:1581	C16 FA	1576:1581	In both species, COS and MAP lowered the yields (mmol/d per kilogram of body weight) of <C16 and C16 FA.
30343921	1	121	theme	fat	287:289	arg1	depression					291:300	milk fat depression	282:300	milk fat depression	282:300	A direct comparison of cow and goat performance and milk fatty acid (FA) responses to diets that either induce milk fat depression or increase milk fat content in cows suggests species-specific regulation of lipid metabolism, including mammary lipogenesis.
30343921	13	122	from	cows	2501:2504	arg1	abundant					2489:2496	abundant	2489:2496	abundant	2489:2496	However, the abundance of 14 mRNA among the 21 encoding for genes studied in the mammary gland was significantly different among species, with 5 more abundant in cows (FADS3, ACSL1, PPARA, LXRA, and PPARG1) and 10 more abundant in goats (FASN, CD36, FABP3, LPL, GPAM, LPIN1, CSN2, MFGE8, and INSIG1).
30343921	3	123	theme	algae	786:790	arg1	DMI					810:812	MAP; 1.5% DMI	800:812	MAP; 1.5% DMI	800:812	Twelve Holstein cows and 12 Alpine goats were fed a basal diet containing no additional lipid (CTL) or a similar diet supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.
30343921	3	123	theme	algae	786:790	arg1	powder					792:797	marine algae powder	779:797	marine algae powder (MAP; 1.5% DMI)	779:813	Twelve Holstein cows and 12 Alpine goats were fed a basal diet containing no additional lipid (CTL) or a similar diet supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.
30343921	11	124	theme	FA	1879:1880	arg1	secretion					1882:1890	FA secretion	1879:1890	FA secretion	1879:1890	These variations in milk fat content and FA secretion were not associated with modifications in the mammary expression of 21 genes involved in major lipid pathways, except for 3 transcription factors: PPARA, INSIG1, and SP1.
30343921	0	125	theme	palm	85:88	arg1	oil					90:92	hydrogenated palm oil	72:92	hydrogenated palm oil	72:92	Effect of diets supplemented with starch and corn oil, marine algae, or hydrogenated palm oil on mammary lipogenic gene expression in cows and goats: A comparative study.
30343921	10	126	theme	body	1816:1819	arg1	weight					1821:1826	body weight	1816:1826	body weight	1816:1826	Supplementation of HPO increased the yield of milk C16 FA (mmol/d per kilogram of body weight) in cows.
30343921	13	127	dep	FASN	2577:2580	arg1	LPL					2596:2598	LPL	2596:2598	LPL	2596:2598	However, the abundance of 14 mRNA among the 21 encoding for genes studied in the mammary gland was significantly different among species, with 5 more abundant in cows (FADS3, ACSL1, PPARA, LXRA, and PPARG1) and 10 more abundant in goats (FASN, CD36, FABP3, LPL, GPAM, LPIN1, CSN2, MFGE8, and INSIG1).
30343921	13	127	dep	FASN	2577:2580	arg1	CSN2					2614:2617	CSN2	2614:2617	CSN2	2614:2617	However, the abundance of 14 mRNA among the 21 encoding for genes studied in the mammary gland was significantly different among species, with 5 more abundant in cows (FADS3, ACSL1, PPARA, LXRA, and PPARG1) and 10 more abundant in goats (FASN, CD36, FABP3, LPL, GPAM, LPIN1, CSN2, MFGE8, and INSIG1).
30343921	13	127	dep	FASN	2577:2580	arg1	MFGE8					2620:2624	MFGE8	2620:2624	MFGE8	2620:2624	However, the abundance of 14 mRNA among the 21 encoding for genes studied in the mammary gland was significantly different among species, with 5 more abundant in cows (FADS3, ACSL1, PPARA, LXRA, and PPARG1) and 10 more abundant in goats (FASN, CD36, FABP3, LPL, GPAM, LPIN1, CSN2, MFGE8, and INSIG1).
30343921	13	127	dep	FASN	2577:2580	arg1	CD36					2583:2586	CD36	2583:2586	CD36	2583:2586	However, the abundance of 14 mRNA among the 21 encoding for genes studied in the mammary gland was significantly different among species, with 5 more abundant in cows (FADS3, ACSL1, PPARA, LXRA, and PPARG1) and 10 more abundant in goats (FASN, CD36, FABP3, LPL, GPAM, LPIN1, CSN2, MFGE8, and INSIG1).
30343921	13	127	dep	FASN	2577:2580	arg1	GPAM					2601:2604	GPAM	2601:2604	GPAM	2601:2604	However, the abundance of 14 mRNA among the 21 encoding for genes studied in the mammary gland was significantly different among species, with 5 more abundant in cows (FADS3, ACSL1, PPARA, LXRA, and PPARG1) and 10 more abundant in goats (FASN, CD36, FABP3, LPL, GPAM, LPIN1, CSN2, MFGE8, and INSIG1).
30343921	13	127	dep	FASN	2577:2580	arg1	INSIG1					2631:2636	INSIG1	2631:2636	INSIG1	2631:2636	However, the abundance of 14 mRNA among the 21 encoding for genes studied in the mammary gland was significantly different among species, with 5 more abundant in cows (FADS3, ACSL1, PPARA, LXRA, and PPARG1) and 10 more abundant in goats (FASN, CD36, FABP3, LPL, GPAM, LPIN1, CSN2, MFGE8, and INSIG1).
30343921	13	127	dep	FASN	2577:2580	arg1	FABP3					2589:2593	FABP3	2589:2593	FABP3	2589:2593	However, the abundance of 14 mRNA among the 21 encoding for genes studied in the mammary gland was significantly different among species, with 5 more abundant in cows (FADS3, ACSL1, PPARA, LXRA, and PPARG1) and 10 more abundant in goats (FASN, CD36, FABP3, LPL, GPAM, LPIN1, CSN2, MFGE8, and INSIG1).
30343921	9	128	from	cows	1670:1673	arg1	increase					1630:1637	an increase	1627:1637	an increase of >C16 FA in goats but not in cows	1627:1673	With COS, this decrease was compensated by an increase of >C16 FA in goats but not in cows, and the >C16 FA yield decreased with MAP in both species.
30343921	1	129	theme	fatty	228:232	arg1	acid					234:237	milk fatty acid	223:237	milk fatty acid (FA)	223:242	A direct comparison of cow and goat performance and milk fatty acid (FA) responses to diets that either induce milk fat depression or increase milk fat content in cows suggests species-specific regulation of lipid metabolism, including mammary lipogenesis.
30343921	1	129	theme	fatty	228:232	arg1	FA					240:241	FA	240:241	FA	240:241	A direct comparison of cow and goat performance and milk fatty acid (FA) responses to diets that either induce milk fat depression or increase milk fat content in cows suggests species-specific regulation of lipid metabolism, including mammary lipogenesis.
30343921	10	130	from	FA	1789:1790	arg1	cows					1832:1835	cows	1832:1835	cows	1832:1835	Supplementation of HPO increased the yield of milk C16 FA (mmol/d per kilogram of body weight) in cows.
30343921	5	131	theme	genes	1063:1067	arg1	abundance					1047:1055	the mRNA abundance	1038:1055	the mRNA abundance of 21 genes involved in lipid metabolism or enzyme activities or both	1038:1125	On d 27 of each experimental period, the mRNA abundance of 21 genes involved in lipid metabolism or enzyme activities or both were measured in mammary tissue sampled by biopsy.
30343921	12	132	theme	corresponding	2315:2327	arg1	proteins					2329:2336	the corresponding proteins	2311:2336	the corresponding proteins	2311:2336	This absence of large changes might be due to post-transcriptional regulation of these genes and related to the time of sampling of the mammary tissue relative to the previous meal and milking or to differences in the availability of substrate for the corresponding proteins.
30343921	3	133	theme	square	883:888	arg1	design					890:895	a 4 × 4 Latin square design	869:895	a 4 × 4 Latin square design with 28-d experimental periods	869:926	Twelve Holstein cows and 12 Alpine goats were fed a basal diet containing no additional lipid (CTL) or a similar diet supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.
30343921	7	134	contain	had	1422:1424	arg2	effect					1429:1434	an effect	1426:1434	an effect	1426:1434	In cows, fat content was lowered by COS (-45%) and MAP (-22%) and increased by HPO (+13%) compared with CTL, and in goats only MAP had an effect compared with CTL, with a decrease of 15%.
30343921	7	134	contain	had	1422:1424	arg1	MAP					1418:1420	only MAP	1413:1420	only MAP	1413:1420	In cows, fat content was lowered by COS (-45%) and MAP (-22%) and increased by HPO (+13%) compared with CTL, and in goats only MAP had an effect compared with CTL, with a decrease of 15%.
30343921	3	135	theme	Holstein	588:595	arg1	cows					597:600	Twelve Holstein cows	581:600	Twelve Holstein cows	581:600	Twelve Holstein cows and 12 Alpine goats were fed a basal diet containing no additional lipid (CTL) or a similar diet supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.
30343921	13	136	dep	abundant	2558:2565	arg1	10					2550:2551	10	2550:2551	10	2550:2551	However, the abundance of 14 mRNA among the 21 encoding for genes studied in the mammary gland was significantly different among species, with 5 more abundant in cows (FADS3, ACSL1, PPARA, LXRA, and PPARG1) and 10 more abundant in goats (FASN, CD36, FABP3, LPL, GPAM, LPIN1, CSN2, MFGE8, and INSIG1).
30343921	0	137	theme	corn	45:48	arg1	oil					50:52	corn oil	45:52	corn oil	45:52	Effect of diets supplemented with starch and corn oil, marine algae, or hydrogenated palm oil on mammary lipogenic gene expression in cows and goats: A comparative study.
30343921	3	138	theme	experimental	907:918	arg1	periods					920:926	28-d experimental periods	902:926	28-d experimental periods	902:926	Twelve Holstein cows and 12 Alpine goats were fed a basal diet containing no additional lipid (CTL) or a similar diet supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.
30343921	6	139	from	differences	1209:1219	arg1	response					1237:1244	the milk fat response	1224:1244	the milk fat response of cows and goats to the dietary treatments	1224:1288	The results showed significant differences in the milk fat response of cows and goats to the dietary treatments.
30343921	3	140	theme	%	808:808	arg1	DMI					810:812	MAP; 1.5% DMI	800:812	MAP; 1.5% DMI	800:812	Twelve Holstein cows and 12 Alpine goats were fed a basal diet containing no additional lipid (CTL) or a similar diet supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.
30343921	3	140	theme	%	808:808	arg1	powder					792:797	marine algae powder	779:797	marine algae powder (MAP; 1.5% DMI)	779:813	Twelve Holstein cows and 12 Alpine goats were fed a basal diet containing no additional lipid (CTL) or a similar diet supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.
30343921	3	141	theme	Alpine	609:614	arg1	goats					616:620	12 Alpine goats	606:620	12 Alpine goats	606:620	Twelve Holstein cows and 12 Alpine goats were fed a basal diet containing no additional lipid (CTL) or a similar diet supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.
30343921	7	142	dep	increased	1357:1365	arg1	compared					1381:1388	compared	1381:1388	compared with CTL	1381:1397	In cows, fat content was lowered by COS (-45%) and MAP (-22%) and increased by HPO (+13%) compared with CTL, and in goats only MAP had an effect compared with CTL, with a decrease of 15%.
30343921	5	143	theme	enzyme	1101:1106	arg1	activities					1108:1117	enzyme activities	1101:1117	enzyme activities	1101:1117	On d 27 of each experimental period, the mRNA abundance of 21 genes involved in lipid metabolism or enzyme activities or both were measured in mammary tissue sampled by biopsy.
30343921	6	144	theme	fat	1233:1235	arg1	response					1237:1244	the milk fat response	1224:1244	the milk fat response of cows and goats to the dietary treatments	1224:1288	The results showed significant differences in the milk fat response of cows and goats to the dietary treatments.
30343921	3	145	theme	palm	832:835	arg1	oil					837:839	hydrogenated palm oil	819:839	hydrogenated palm oil (HPO; 3% DMI)	819:853	Twelve Holstein cows and 12 Alpine goats were fed a basal diet containing no additional lipid (CTL) or a similar diet supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.
30343921	3	145	theme	palm	832:835	arg1	DMI					850:852	HPO; 3% DMI	842:852	HPO; 3% DMI	842:852	Twelve Holstein cows and 12 Alpine goats were fed a basal diet containing no additional lipid (CTL) or a similar diet supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.
30343921	1	146	theme	cow	194:196	arg1	comparison					180:189	A direct comparison	171:189	A direct comparison of cow and goat performance and milk fatty acid (FA) responses to diets that either induce milk fat depression or increase milk fat content in cows	171:337	A direct comparison of cow and goat performance and milk fatty acid (FA) responses to diets that either induce milk fat depression or increase milk fat content in cows suggests species-specific regulation of lipid metabolism, including mammary lipogenesis.
29706978	0	0	theme	Gynoecium	80:88	arg1	Domain					97:102	the Gynoecium Medial Domain	76:102	the Gynoecium Medial Domain	76:102	Exploring Cell Wall Composition and Modifications During the Development of the Gynoecium Medial Domain in Arabidopsis.
29706978	6	1	theme	distribution	1278:1289	arg1	formation					1155:1163	the formation	1151:1163	the formation of the transmitting tract	1151:1189	We detected coordinated changes in polysaccharide distribution through time, cell wall modifications preceding the formation of the transmitting tract, mucosubstances increase during transmitting tract formation, and a decrease of mannan distribution.
29706978	6	1	theme	distribution	1278:1289	arg1	increase					1207:1214	mucosubstances increase	1192:1214	mucosubstances increase during transmitting tract formation	1192:1250	We detected coordinated changes in polysaccharide distribution through time, cell wall modifications preceding the formation of the transmitting tract, mucosubstances increase during transmitting tract formation, and a decrease of mannan distribution.
29706978	6	1	theme	distribution	1278:1289	arg1	decrease					1259:1266	a decrease	1257:1266	a decrease of mannan distribution	1257:1289	We detected coordinated changes in polysaccharide distribution through time, cell wall modifications preceding the formation of the transmitting tract, mucosubstances increase during transmitting tract formation, and a decrease of mannan distribution.
29706978	8	2	theme	postgenital	1439:1449	arg1	fusion					1451:1456	postgenital fusion	1439:1456	postgenital fusion of the carpel (septum fusion)	1439:1486	Proper cell wall composition and modifications are important for postgenital fusion of the carpel (septum fusion) and transmitting tract formation, because these tissues affect plant reproductive competence.
29706978	7	3	theme	septum	1359:1364	arg1	fusion					1366:1371	septum fusion	1359:1371	septum fusion	1359:1371	Furthermore, we also detected changes in lipid distribution during septum fusion.
29706978	6	4	theme	mannan	1271:1276	arg1	distribution					1278:1289	mannan distribution	1271:1289	mannan distribution	1271:1289	We detected coordinated changes in polysaccharide distribution through time, cell wall modifications preceding the formation of the transmitting tract, mucosubstances increase during transmitting tract formation, and a decrease of mannan distribution.
29706978	4	5	dep	impact	696:701	arg1	facilitates					741:751	facilitates	741:751	facilitates pollen tube growth and movement through the ovary	741:801	During gynoecium development, cell wall modifications take place and one of the most important is the formation of the transmitting tract, having a great impact on reproductive competence because it facilitates pollen tube growth and movement through the ovary.
29706978	8	6	theme	Proper	1374:1379	arg1	composition					1391:1401	Proper cell wall composition	1374:1401	Proper cell wall composition	1374:1401	Proper cell wall composition and modifications are important for postgenital fusion of the carpel (septum fusion) and transmitting tract formation, because these tissues affect plant reproductive competence.
29706978	0	7	theme	Domain	97:102	arg1	Development					61:71	the Development	57:71	the Development of the Gynoecium Medial Domain in Arabidopsis	57:117	Exploring Cell Wall Composition and Modifications During the Development of the Gynoecium Medial Domain in Arabidopsis.
29706978	5	8	theme	cell	933:936	arg1	composition					943:953	cell wall composition	933:953	cell wall composition	933:953	In this study, using a combination of classical staining methods, fluorescent dyes, and indirect immunolocalization, we analyzed cell wall composition and modifications accompanying medial domain formation during gynoecium development.
29706978	5	9	theme	indirect	892:899	arg1	immunolocalization					901:918	indirect immunolocalization	892:918	indirect immunolocalization	892:918	In this study, using a combination of classical staining methods, fluorescent dyes, and indirect immunolocalization, we analyzed cell wall composition and modifications accompanying medial domain formation during gynoecium development.
29706978	6	10	theme	cell	1117:1120	arg1	modifications					1127:1139	cell wall modifications	1117:1139	cell wall modifications preceding the formation of the transmitting tract, mucosubstances increase during transmitting tract formation, and a decrease of mannan distribution	1117:1289	We detected coordinated changes in polysaccharide distribution through time, cell wall modifications preceding the formation of the transmitting tract, mucosubstances increase during transmitting tract formation, and a decrease of mannan distribution.
29706978	6	10	theme	cell	1117:1120	arg1	time					1111:1114	time	1111:1114	time	1111:1114	We detected coordinated changes in polysaccharide distribution through time, cell wall modifications preceding the formation of the transmitting tract, mucosubstances increase during transmitting tract formation, and a decrease of mannan distribution.
29706978	4	11	theme	gynoecium	549:557	arg1	development					559:569	gynoecium development	549:569	gynoecium development	549:569	During gynoecium development, cell wall modifications take place and one of the most important is the formation of the transmitting tract, having a great impact on reproductive competence because it facilitates pollen tube growth and movement through the ovary.
29706978	0	12	theme	Medial	90:95	arg1	Domain					97:102	the Gynoecium Medial Domain	76:102	the Gynoecium Medial Domain	76:102	Exploring Cell Wall Composition and Modifications During the Development of the Gynoecium Medial Domain in Arabidopsis.
29706978	5	13	theme	immunolocalization	901:918	arg1	combination					827:837	a combination	825:837	a combination of classical staining methods, fluorescent dyes, and indirect immunolocalization	825:918	In this study, using a combination of classical staining methods, fluorescent dyes, and indirect immunolocalization, we analyzed cell wall composition and modifications accompanying medial domain formation during gynoecium development.
29706978	4	14	theme	transmitting	661:672	arg1	tract					674:678	the transmitting tract	657:678	the transmitting tract	657:678	During gynoecium development, cell wall modifications take place and one of the most important is the formation of the transmitting tract, having a great impact on reproductive competence because it facilitates pollen tube growth and movement through the ovary.
29706978	5	15	theme	classical	842:850	arg1	methods					861:867	classical staining methods	842:867	classical staining methods	842:867	In this study, using a combination of classical staining methods, fluorescent dyes, and indirect immunolocalization, we analyzed cell wall composition and modifications accompanying medial domain formation during gynoecium development.
29706978	4	16	from	impact	696:701	arg1	competence					719:728	reproductive competence	706:728	reproductive competence	706:728	During gynoecium development, cell wall modifications take place and one of the most important is the formation of the transmitting tract, having a great impact on reproductive competence because it facilitates pollen tube growth and movement through the ovary.
29706978	5	17	theme	methods	861:867	arg1	combination					827:837	a combination	825:837	a combination of classical staining methods, fluorescent dyes, and indirect immunolocalization	825:918	In this study, using a combination of classical staining methods, fluorescent dyes, and indirect immunolocalization, we analyzed cell wall composition and modifications accompanying medial domain formation during gynoecium development.
29706978	1	18	theme	inner	155:159	arg1	gynoecium					140:148	the gynoecium	136:148	the gynoecium	136:148	In Arabidopsis, the gynoecium, the inner whorl of the flower, is the female reproductive part.
29706978	1	18	theme	inner	155:159	arg1	whorl					161:165	the inner whorl	151:165	the inner whorl of the flower	151:179	In Arabidopsis, the gynoecium, the inner whorl of the flower, is the female reproductive part.
29706978	4	19	theme	reproductive	706:717	arg1	competence					719:728	reproductive competence	706:728	reproductive competence	706:728	During gynoecium development, cell wall modifications take place and one of the most important is the formation of the transmitting tract, having a great impact on reproductive competence because it facilitates pollen tube growth and movement through the ovary.
29706978	6	20	theme	coordinated	1052:1062	arg1	changes					1064:1070	coordinated changes	1052:1070	coordinated changes in polysaccharide distribution	1052:1101	We detected coordinated changes in polysaccharide distribution through time, cell wall modifications preceding the formation of the transmitting tract, mucosubstances increase during transmitting tract formation, and a decrease of mannan distribution.
29706978	2	21	theme	margin	385:390	arg1	meristem					392:399	the carpel margin meristem	374:399	the carpel margin meristem	374:399	Many tissues important for fertilization such as the stigma, style, transmitting tract, placenta, ovules, and septum, comprising the medial domain, arise from the carpel margin meristem.
29706978	8	22	theme	plant	1551:1555	arg1	competence					1570:1579	plant reproductive competence	1551:1579	plant reproductive competence	1551:1579	Proper cell wall composition and modifications are important for postgenital fusion of the carpel (septum fusion) and transmitting tract formation, because these tissues affect plant reproductive competence.
29706978	5	23	theme	fluorescent	870:880	arg1	dyes					882:885	fluorescent dyes	870:885	fluorescent dyes	870:885	In this study, using a combination of classical staining methods, fluorescent dyes, and indirect immunolocalization, we analyzed cell wall composition and modifications accompanying medial domain formation during gynoecium development.
29706978	2	24	theme	carpel	378:383	arg1	meristem					392:399	the carpel margin meristem	374:399	the carpel margin meristem	374:399	Many tissues important for fertilization such as the stigma, style, transmitting tract, placenta, ovules, and septum, comprising the medial domain, arise from the carpel margin meristem.
29706978	6	25	theme	tract	1185:1189	arg1	formation					1155:1163	the formation	1151:1163	the formation of the transmitting tract	1151:1189	We detected coordinated changes in polysaccharide distribution through time, cell wall modifications preceding the formation of the transmitting tract, mucosubstances increase during transmitting tract formation, and a decrease of mannan distribution.
29706978	6	25	theme	tract	1185:1189	arg1	increase					1207:1214	mucosubstances increase	1192:1214	mucosubstances increase during transmitting tract formation	1192:1250	We detected coordinated changes in polysaccharide distribution through time, cell wall modifications preceding the formation of the transmitting tract, mucosubstances increase during transmitting tract formation, and a decrease of mannan distribution.
29706978	6	25	theme	tract	1185:1189	arg1	decrease					1259:1266	a decrease	1257:1266	a decrease of mannan distribution	1257:1289	We detected coordinated changes in polysaccharide distribution through time, cell wall modifications preceding the formation of the transmitting tract, mucosubstances increase during transmitting tract formation, and a decrease of mannan distribution.
29706978	5	26	theme	dyes	882:885	arg1	combination					827:837	a combination	825:837	a combination of classical staining methods, fluorescent dyes, and indirect immunolocalization	825:918	In this study, using a combination of classical staining methods, fluorescent dyes, and indirect immunolocalization, we analyzed cell wall composition and modifications accompanying medial domain formation during gynoecium development.
29706978	5	27	theme	wall	938:941	arg1	composition					943:953	cell wall composition	933:953	cell wall composition	933:953	In this study, using a combination of classical staining methods, fluorescent dyes, and indirect immunolocalization, we analyzed cell wall composition and modifications accompanying medial domain formation during gynoecium development.
29706978	4	28	theme	great	690:694	arg1	impact					696:701	a great impact	688:701	a great impact on reproductive competence because it facilitates pollen tube growth and movement through the ovary	688:801	During gynoecium development, cell wall modifications take place and one of the most important is the formation of the transmitting tract, having a great impact on reproductive competence because it facilitates pollen tube growth and movement through the ovary.
29706978	1	29	theme	flower	174:179	arg1	gynoecium					140:148	the gynoecium	136:148	the gynoecium	136:148	In Arabidopsis, the gynoecium, the inner whorl of the flower, is the female reproductive part.
29706978	1	29	theme	flower	174:179	arg1	whorl					161:165	the inner whorl	151:165	the inner whorl of the flower	151:179	In Arabidopsis, the gynoecium, the inner whorl of the flower, is the female reproductive part.
29706978	0	30	theme	Wall	15:18	arg1	Composition					20:30	Cell Wall Composition	10:30	Cell Wall Composition	10:30	Exploring Cell Wall Composition and Modifications During the Development of the Gynoecium Medial Domain in Arabidopsis.
29706978	8	31	theme	wall	1386:1389	arg1	composition					1391:1401	Proper cell wall composition	1374:1401	Proper cell wall composition	1374:1401	Proper cell wall composition and modifications are important for postgenital fusion of the carpel (septum fusion) and transmitting tract formation, because these tissues affect plant reproductive competence.
29706978	3	32	theme	successful	500:509	arg1	pollination					511:521	a successful pollination	498:521	a successful pollination	498:521	During gynoecium development, septum fusion occurs and tissues form continuously to prepare for a successful pollination and fertilization.
29706978	3	33	theme	gynoecium	409:417	arg1	development					419:429	gynoecium development	409:429	gynoecium development	409:429	During gynoecium development, septum fusion occurs and tissues form continuously to prepare for a successful pollination and fertilization.
29706978	8	34	theme	reproductive	1557:1568	arg1	competence					1570:1579	plant reproductive competence	1551:1579	plant reproductive competence	1551:1579	Proper cell wall composition and modifications are important for postgenital fusion of the carpel (septum fusion) and transmitting tract formation, because these tissues affect plant reproductive competence.
29706978	6	35	theme	tract	1236:1240	arg1	formation					1242:1250	transmitting tract formation	1223:1250	transmitting tract formation	1223:1250	We detected coordinated changes in polysaccharide distribution through time, cell wall modifications preceding the formation of the transmitting tract, mucosubstances increase during transmitting tract formation, and a decrease of mannan distribution.
29706978	6	36	theme	transmitting	1172:1183	arg1	tract					1185:1189	the transmitting tract	1168:1189	the transmitting tract	1168:1189	We detected coordinated changes in polysaccharide distribution through time, cell wall modifications preceding the formation of the transmitting tract, mucosubstances increase during transmitting tract formation, and a decrease of mannan distribution.
29706978	8	37	theme	carpel	1465:1470	arg1	fusion					1451:1456	postgenital fusion	1439:1456	postgenital fusion of the carpel (septum fusion)	1439:1486	Proper cell wall composition and modifications are important for postgenital fusion of the carpel (septum fusion) and transmitting tract formation, because these tissues affect plant reproductive competence.
29706978	8	37	theme	carpel	1465:1470	arg1	formation					1511:1519	transmitting tract formation	1492:1519	transmitting tract formation	1492:1519	Proper cell wall composition and modifications are important for postgenital fusion of the carpel (septum fusion) and transmitting tract formation, because these tissues affect plant reproductive competence.
29706978	4	38	theme	tube	760:763	arg1	growth					765:770	pollen tube growth	753:770	pollen tube growth	753:770	During gynoecium development, cell wall modifications take place and one of the most important is the formation of the transmitting tract, having a great impact on reproductive competence because it facilitates pollen tube growth and movement through the ovary.
29706978	2	39	theme	medial	348:353	arg1	domain					355:360	the medial domain	344:360	the medial domain	344:360	Many tissues important for fertilization such as the stigma, style, transmitting tract, placenta, ovules, and septum, comprising the medial domain, arise from the carpel margin meristem.
29706978	8	40	theme	septum	1473:1478	arg1	carpel					1465:1470	the carpel	1461:1470	the carpel (septum fusion)	1461:1486	Proper cell wall composition and modifications are important for postgenital fusion of the carpel (septum fusion) and transmitting tract formation, because these tissues affect plant reproductive competence.
29706978	8	40	theme	septum	1473:1478	arg1	fusion					1480:1485	septum fusion	1473:1485	septum fusion	1473:1485	Proper cell wall composition and modifications are important for postgenital fusion of the carpel (septum fusion) and transmitting tract formation, because these tissues affect plant reproductive competence.
29706978	1	41	from	part	209:212	arg1	Arabidopsis					123:133	Arabidopsis	123:133	Arabidopsis	123:133	In Arabidopsis, the gynoecium, the inner whorl of the flower, is the female reproductive part.
29706978	0	42	from	Development	61:71	arg1	Arabidopsis					107:117	Arabidopsis	107:117	Arabidopsis	107:117	Exploring Cell Wall Composition and Modifications During the Development of the Gynoecium Medial Domain in Arabidopsis.
29706978	7	43	theme	lipid	1333:1337	arg1	distribution					1339:1350	lipid distribution	1333:1350	lipid distribution	1333:1350	Furthermore, we also detected changes in lipid distribution during septum fusion.
29706978	3	44	theme	septum	432:437	arg1	fusion					439:444	septum fusion	432:444	septum fusion	432:444	During gynoecium development, septum fusion occurs and tissues form continuously to prepare for a successful pollination and fertilization.
29706978	8	45	theme	tract	1505:1509	arg1	formation					1511:1519	transmitting tract formation	1492:1519	transmitting tract formation	1492:1519	Proper cell wall composition and modifications are important for postgenital fusion of the carpel (septum fusion) and transmitting tract formation, because these tissues affect plant reproductive competence.
29706978	5	46	theme	domain	993:998	arg1	formation					1000:1008	medial domain formation	986:1008	medial domain formation	986:1008	In this study, using a combination of classical staining methods, fluorescent dyes, and indirect immunolocalization, we analyzed cell wall composition and modifications accompanying medial domain formation during gynoecium development.
29706978	5	47	theme	medial	986:991	arg1	formation					1000:1008	medial domain formation	986:1008	medial domain formation	986:1008	In this study, using a combination of classical staining methods, fluorescent dyes, and indirect immunolocalization, we analyzed cell wall composition and modifications accompanying medial domain formation during gynoecium development.
29706978	5	48	theme	staining	852:859	arg1	methods					861:867	classical staining methods	842:867	classical staining methods	842:867	In this study, using a combination of classical staining methods, fluorescent dyes, and indirect immunolocalization, we analyzed cell wall composition and modifications accompanying medial domain formation during gynoecium development.
29706978	4	49	dep	one	611:613	arg1	important					627:635	important	627:635	important	627:635	During gynoecium development, cell wall modifications take place and one of the most important is the formation of the transmitting tract, having a great impact on reproductive competence because it facilitates pollen tube growth and movement through the ovary.
29706978	4	49	dep	one	611:613	arg1	formation					644:652	the formation	640:652	the formation of the transmitting tract	640:678	During gynoecium development, cell wall modifications take place and one of the most important is the formation of the transmitting tract, having a great impact on reproductive competence because it facilitates pollen tube growth and movement through the ovary.
29706978	6	50	theme	mucosubstances	1192:1205	arg1	increase					1207:1214	mucosubstances increase	1192:1214	mucosubstances increase during transmitting tract formation	1192:1250	We detected coordinated changes in polysaccharide distribution through time, cell wall modifications preceding the formation of the transmitting tract, mucosubstances increase during transmitting tract formation, and a decrease of mannan distribution.
29706978	6	51	theme	transmitting	1223:1234	arg1	formation					1242:1250	transmitting tract formation	1223:1250	transmitting tract formation	1223:1250	We detected coordinated changes in polysaccharide distribution through time, cell wall modifications preceding the formation of the transmitting tract, mucosubstances increase during transmitting tract formation, and a decrease of mannan distribution.
29706978	4	52	theme	tract	674:678	arg1	important					627:635	important	627:635	important	627:635	During gynoecium development, cell wall modifications take place and one of the most important is the formation of the transmitting tract, having a great impact on reproductive competence because it facilitates pollen tube growth and movement through the ovary.
29706978	4	52	theme	tract	674:678	arg1	formation					644:652	the formation	640:652	the formation of the transmitting tract	640:678	During gynoecium development, cell wall modifications take place and one of the most important is the formation of the transmitting tract, having a great impact on reproductive competence because it facilitates pollen tube growth and movement through the ovary.
29706978	6	53	theme	wall	1122:1125	arg1	modifications					1127:1139	cell wall modifications	1117:1139	cell wall modifications preceding the formation of the transmitting tract, mucosubstances increase during transmitting tract formation, and a decrease of mannan distribution	1117:1289	We detected coordinated changes in polysaccharide distribution through time, cell wall modifications preceding the formation of the transmitting tract, mucosubstances increase during transmitting tract formation, and a decrease of mannan distribution.
29706978	6	53	theme	wall	1122:1125	arg1	time					1111:1114	time	1111:1114	time	1111:1114	We detected coordinated changes in polysaccharide distribution through time, cell wall modifications preceding the formation of the transmitting tract, mucosubstances increase during transmitting tract formation, and a decrease of mannan distribution.
29706978	1	54	theme	female	189:194	arg1	part					209:212	the female reproductive part	185:212	the female reproductive part	185:212	In Arabidopsis, the gynoecium, the inner whorl of the flower, is the female reproductive part.
29706978	1	54	theme	female	189:194	arg1	gynoecium					140:148	the gynoecium	136:148	the gynoecium	136:148	In Arabidopsis, the gynoecium, the inner whorl of the flower, is the female reproductive part.
29706978	2	55	theme	important	228:236	arg1	style					276:280	style	276:280	style	276:280	Many tissues important for fertilization such as the stigma, style, transmitting tract, placenta, ovules, and septum, comprising the medial domain, arise from the carpel margin meristem.
29706978	2	55	theme	important	228:236	arg1	ovules					313:318	ovules	313:318	ovules	313:318	Many tissues important for fertilization such as the stigma, style, transmitting tract, placenta, ovules, and septum, comprising the medial domain, arise from the carpel margin meristem.
29706978	2	55	theme	important	228:236	arg1	tract					296:300	transmitting tract	283:300	transmitting tract	283:300	Many tissues important for fertilization such as the stigma, style, transmitting tract, placenta, ovules, and septum, comprising the medial domain, arise from the carpel margin meristem.
29706978	2	55	theme	important	228:236	arg1	tissues					220:226	Many tissues	215:226	Many tissues important for fertilization such as the stigma, style, transmitting tract, placenta, ovules, and septum, comprising the medial domain,	215:361	Many tissues important for fertilization such as the stigma, style, transmitting tract, placenta, ovules, and septum, comprising the medial domain, arise from the carpel margin meristem.
29706978	2	55	theme	important	228:236	arg1	septum					325:330	septum	325:330	septum	325:330	Many tissues important for fertilization such as the stigma, style, transmitting tract, placenta, ovules, and septum, comprising the medial domain, arise from the carpel margin meristem.
29706978	2	55	theme	important	228:236	arg1	placenta					303:310	placenta	303:310	placenta	303:310	Many tissues important for fertilization such as the stigma, style, transmitting tract, placenta, ovules, and septum, comprising the medial domain, arise from the carpel margin meristem.
29706978	2	55	theme	important	228:236	arg1	stigma					268:273	the stigma	264:273	the stigma	264:273	Many tissues important for fertilization such as the stigma, style, transmitting tract, placenta, ovules, and septum, comprising the medial domain, arise from the carpel margin meristem.
29706978	6	56	from	changes	1064:1070	arg1	distribution					1090:1101	polysaccharide distribution	1075:1101	polysaccharide distribution	1075:1101	We detected coordinated changes in polysaccharide distribution through time, cell wall modifications preceding the formation of the transmitting tract, mucosubstances increase during transmitting tract formation, and a decrease of mannan distribution.
29706978	2	57	theme	transmitting	283:294	arg1	tract					296:300	transmitting tract	283:300	transmitting tract	283:300	Many tissues important for fertilization such as the stigma, style, transmitting tract, placenta, ovules, and septum, comprising the medial domain, arise from the carpel margin meristem.
29706978	1	58	theme	reproductive	196:207	arg1	part					209:212	the female reproductive part	185:212	the female reproductive part	185:212	In Arabidopsis, the gynoecium, the inner whorl of the flower, is the female reproductive part.
29706978	1	58	theme	reproductive	196:207	arg1	gynoecium					140:148	the gynoecium	136:148	the gynoecium	136:148	In Arabidopsis, the gynoecium, the inner whorl of the flower, is the female reproductive part.
29706978	5	59	theme	gynoecium	1017:1025	arg1	development					1027:1037	gynoecium development	1017:1037	gynoecium development	1017:1037	In this study, using a combination of classical staining methods, fluorescent dyes, and indirect immunolocalization, we analyzed cell wall composition and modifications accompanying medial domain formation during gynoecium development.
29706978	4	60	theme	pollen	753:758	arg1	growth					765:770	pollen tube growth	753:770	pollen tube growth	753:770	During gynoecium development, cell wall modifications take place and one of the most important is the formation of the transmitting tract, having a great impact on reproductive competence because it facilitates pollen tube growth and movement through the ovary.
29706978	6	61	theme	polysaccharide	1075:1088	arg1	distribution					1090:1101	polysaccharide distribution	1075:1101	polysaccharide distribution	1075:1101	We detected coordinated changes in polysaccharide distribution through time, cell wall modifications preceding the formation of the transmitting tract, mucosubstances increase during transmitting tract formation, and a decrease of mannan distribution.
29706978	8	62	theme	cell	1381:1384	arg1	composition					1391:1401	Proper cell wall composition	1374:1401	Proper cell wall composition	1374:1401	Proper cell wall composition and modifications are important for postgenital fusion of the carpel (septum fusion) and transmitting tract formation, because these tissues affect plant reproductive competence.
29706978	4	63	theme	wall	577:580	arg1	modifications					582:594	cell wall modifications	572:594	cell wall modifications	572:594	During gynoecium development, cell wall modifications take place and one of the most important is the formation of the transmitting tract, having a great impact on reproductive competence because it facilitates pollen tube growth and movement through the ovary.
29706978	8	64	theme	transmitting	1492:1503	arg1	formation					1511:1519	transmitting tract formation	1492:1519	transmitting tract formation	1492:1519	Proper cell wall composition and modifications are important for postgenital fusion of the carpel (septum fusion) and transmitting tract formation, because these tissues affect plant reproductive competence.
29706978	2	65	theme	Many	215:218	arg1	style					276:280	style	276:280	style	276:280	Many tissues important for fertilization such as the stigma, style, transmitting tract, placenta, ovules, and septum, comprising the medial domain, arise from the carpel margin meristem.
29706978	2	65	theme	Many	215:218	arg1	ovules					313:318	ovules	313:318	ovules	313:318	Many tissues important for fertilization such as the stigma, style, transmitting tract, placenta, ovules, and septum, comprising the medial domain, arise from the carpel margin meristem.
29706978	2	65	theme	Many	215:218	arg1	tract					296:300	transmitting tract	283:300	transmitting tract	283:300	Many tissues important for fertilization such as the stigma, style, transmitting tract, placenta, ovules, and septum, comprising the medial domain, arise from the carpel margin meristem.
29706978	2	65	theme	Many	215:218	arg1	tissues					220:226	Many tissues	215:226	Many tissues important for fertilization such as the stigma, style, transmitting tract, placenta, ovules, and septum, comprising the medial domain,	215:361	Many tissues important for fertilization such as the stigma, style, transmitting tract, placenta, ovules, and septum, comprising the medial domain, arise from the carpel margin meristem.
29706978	2	65	theme	Many	215:218	arg1	septum					325:330	septum	325:330	septum	325:330	Many tissues important for fertilization such as the stigma, style, transmitting tract, placenta, ovules, and septum, comprising the medial domain, arise from the carpel margin meristem.
29706978	2	65	theme	Many	215:218	arg1	placenta					303:310	placenta	303:310	placenta	303:310	Many tissues important for fertilization such as the stigma, style, transmitting tract, placenta, ovules, and septum, comprising the medial domain, arise from the carpel margin meristem.
29706978	2	65	theme	Many	215:218	arg1	stigma					268:273	the stigma	264:273	the stigma	264:273	Many tissues important for fertilization such as the stigma, style, transmitting tract, placenta, ovules, and septum, comprising the medial domain, arise from the carpel margin meristem.
29706978	7	66	from	changes	1322:1328	arg1	distribution					1339:1350	lipid distribution	1333:1350	lipid distribution	1333:1350	Furthermore, we also detected changes in lipid distribution during septum fusion.
29706978	0	67	theme	Cell	10:13	arg1	Composition					20:30	Cell Wall Composition	10:30	Cell Wall Composition	10:30	Exploring Cell Wall Composition and Modifications During the Development of the Gynoecium Medial Domain in Arabidopsis.
29706978	4	68	theme	cell	572:575	arg1	modifications					582:594	cell wall modifications	572:594	cell wall modifications	572:594	During gynoecium development, cell wall modifications take place and one of the most important is the formation of the transmitting tract, having a great impact on reproductive competence because it facilitates pollen tube growth and movement through the ovary.
29069405	7	0	dep	ceramides	1171:1179	arg1	tetrasaccharides					1195:1210	tetrasaccharides	1195:1210	tetrasaccharides in larvae	1195:1220	Neutral GSLs such as ceramides di-, tri- and tetrasaccharides in larvae showed less abundant than those in pupae.
29069405	7	0	dep	ceramides	1171:1179	arg1	ceramides					1171:1179	ceramides di-, tri- and tetrasaccharides in larvae	1171:1220	ceramides di-, tri- and tetrasaccharides in larvae	1171:1220	Neutral GSLs such as ceramides di-, tri- and tetrasaccharides in larvae showed less abundant than those in pupae.
29069405	7	0	dep	ceramides	1171:1179	arg1	tri-					1186:1189	tri-	1186:1189	tri-	1186:1189	Neutral GSLs such as ceramides di-, tri- and tetrasaccharides in larvae showed less abundant than those in pupae.
29069405	7	0	dep	ceramides	1171:1179	arg1	di-					1181:1183	di-	1181:1183	di-	1181:1183	Neutral GSLs such as ceramides di-, tri- and tetrasaccharides in larvae showed less abundant than those in pupae.
29069405	9	1	theme	GSLs	1481:1484	arg1	series					1471:1476	a novel series	1463:1476	a novel series of GSLs	1463:1484	The structural analysis of neutral GSLs from silkworms revealed a novel series of GSLs.
29069405	4	2	theme	other	792:796	arg1	Arthropoda					798:807	other Arthropoda	792:807	other Arthropoda	792:807	The arthro-series GSL GlcNAcβ3Manβ4Glcβ1Cer, a characteristic GSL-glycan sequence of other Arthropoda, was detected in silkworms.
29069405	6	3	theme	thin-layer	991:1000	arg1	chromatography					1002:1015	thin-layer chromatography	991:1015	thin-layer chromatography	991:1015	GSL expression patterns in larvae and pupae were compared using thin-layer chromatography, which demonstrated differences among acidic, polar and neutral GSL fractions, while the zwitterionic fraction showed no difference.
29069405	8	4	theme	larval	1300:1305	arg1	GSLs					1307:1310	larval GSLs	1300:1310	larval GSLs	1300:1310	MALDI-TOF MS analysis revealed that larval GSLs contained four types of ceramide species, whereas pupal GSLs contained only two types.
29069405	0	5	from	analysis	11:18	arg1	difference					88:97	the difference	84:97	the difference in ceramide composition between larvae and pupae	84:146	Structural analysis of neutral glycosphingolipids from the silkworm Bombyx mori and the difference in ceramide composition between larvae and pupae.
29069405	0	5	from	analysis	11:18	arg1	silkworm					59:66	the silkworm	55:66	the silkworm Bombyx mori	55:78	Structural analysis of neutral glycosphingolipids from the silkworm Bombyx mori and the difference in ceramide composition between larvae and pupae.
29069405	8	6	contain	contained	1373:1381	arg1	GSLs					1368:1371	pupal GSLs	1362:1371	pupal GSLs	1362:1371	MALDI-TOF MS analysis revealed that larval GSLs contained four types of ceramide species, whereas pupal GSLs contained only two types.
29069405	8	6	contain	contained	1373:1381	arg2	types					1392:1396	only two types	1383:1396	only two types	1383:1396	MALDI-TOF MS analysis revealed that larval GSLs contained four types of ceramide species, whereas pupal GSLs contained only two types.
29069405	8	7	theme	MALDI-TOF	1264:1272	arg1	analysis					1277:1284	MALDI-TOF MS analysis	1264:1284	MALDI-TOF MS analysis	1264:1284	MALDI-TOF MS analysis revealed that larval GSLs contained four types of ceramide species, whereas pupal GSLs contained only two types.
29069405	4	8	located	detected	814:821	arg1	silkworms					826:834	silkworms	826:834	silkworms	826:834	The arthro-series GSL GlcNAcβ3Manβ4Glcβ1Cer, a characteristic GSL-glycan sequence of other Arthropoda, was detected in silkworms.
29069405	4	8	located	detected	814:821	arg2	sequence					780:787	a characteristic GSL-glycan sequence	752:787	a characteristic GSL-glycan sequence of other Arthropoda	752:807	The arthro-series GSL GlcNAcβ3Manβ4Glcβ1Cer, a characteristic GSL-glycan sequence of other Arthropoda, was detected in silkworms.
29069405	4	8	located	detected	814:821	arg2	GlcNAcβ3Manβ4Glcβ1Cer					729:749	The arthro-series GSL GlcNAcβ3Manβ4Glcβ1Cer	707:749	The arthro-series GSL GlcNAcβ3Manβ4Glcβ1Cer	707:749	The arthro-series GSL GlcNAcβ3Manβ4Glcβ1Cer, a characteristic GSL-glycan sequence of other Arthropoda, was detected in silkworms.
29069405	4	9	theme	GSL-glycan	769:778	arg1	sequence					780:787	a characteristic GSL-glycan sequence	752:787	a characteristic GSL-glycan sequence of other Arthropoda	752:807	The arthro-series GSL GlcNAcβ3Manβ4Glcβ1Cer, a characteristic GSL-glycan sequence of other Arthropoda, was detected in silkworms.
29069405	4	9	theme	GSL-glycan	769:778	arg1	GlcNAcβ3Manβ4Glcβ1Cer					729:749	The arthro-series GSL GlcNAcβ3Manβ4Glcβ1Cer	707:749	The arthro-series GSL GlcNAcβ3Manβ4Glcβ1Cer	707:749	The arthro-series GSL GlcNAcβ3Manβ4Glcβ1Cer, a characteristic GSL-glycan sequence of other Arthropoda, was detected in silkworms.
29069405	7	10	from	tri-	1186:1189	arg1	larvae					1215:1220	larvae	1215:1220	larvae	1215:1220	Neutral GSLs such as ceramides di-, tri- and tetrasaccharides in larvae showed less abundant than those in pupae.
29069405	11	11	theme	GSL	1616:1618	arg1	composition					1629:1639	GSL ceramide composition	1616:1639	GSL ceramide composition between larvae and pupae	1616:1664	Alterations in GSL ceramide composition between larvae and pupae were observed by MALDI-TOF MS analysis.
29069405	3	12	theme	non-reducing	636:647	arg1	end					649:651	the non-reducing end	632:651	the non-reducing end of Galβ3Manβ4Glcβ1Cer with up to five GalNAc repeats	632:704	Lin-ear elongation of α4-GalNAc was observed at the non-reducing end of Galβ3Manβ4Glcβ1Cer with up to five GalNAc repeats.
29069405	0	13	from	silkworm	59:66	arg1	analysis					11:18	Structural analysis	0:18	Structural analysis of neutral glycosphingolipids from the silkworm Bombyx mori and the difference in ceramide composition between larvae and pupae.	0:147	Structural analysis of neutral glycosphingolipids from the silkworm Bombyx mori and the difference in ceramide composition between larvae and pupae.
29069405	0	13	from	silkworm	59:66	arg1	glycosphingolipids					31:48	neutral glycosphingolipids	23:48	neutral glycosphingolipids from the silkworm Bombyx mori and the difference in ceramide composition between larvae and pupae	23:146	Structural analysis of neutral glycosphingolipids from the silkworm Bombyx mori and the difference in ceramide composition between larvae and pupae.
29069405	0	13	from	silkworm	59:66	arg1	composition					111:121	ceramide composition	102:121	ceramide composition	102:121	Structural analysis of neutral glycosphingolipids from the silkworm Bombyx mori and the difference in ceramide composition between larvae and pupae.
29069405	10	14	theme	patterns	1520:1527	arg1	comparison					1491:1500	The comparison	1487:1500	The comparison of GSL expression patterns between larvae and pupae	1487:1552	The comparison of GSL expression patterns between larvae and pupae demonstrated differences in several fractions.
29069405	2	15	from	pupae	340:344	arg1	analysis					304:311	The structural analysis	289:311	The structural analysis of neutral GSLs from dried pupae	289:344	The structural analysis of neutral GSLs from dried pupae revealed the following predominant species: Glcβ1Cer, Manβ4Glcβ1Cer, GlcNAcβ3Manβ4Glcβ1Cer, Galβ3Manβ4Glcβ1Cer, GalNAcα4Galβ3Manβ4Glcβ1Cer, GlcNAcβ3Galβ3Manβ4Glcβ1Cer, Galα4Galβ3Manβ4Glcβ1Cer and (GalNAcα4)1-4 GalNAcα4Galβ3Manβ4Glcβ1Cer.
29069405	2	15	from	pupae	340:344	arg1	GSLs					324:327	neutral GSLs	316:327	neutral GSLs from dried pupae	316:344	The structural analysis of neutral GSLs from dried pupae revealed the following predominant species: Glcβ1Cer, Manβ4Glcβ1Cer, GlcNAcβ3Manβ4Glcβ1Cer, Galβ3Manβ4Glcβ1Cer, GalNAcα4Galβ3Manβ4Glcβ1Cer, GlcNAcβ3Galβ3Manβ4Glcβ1Cer, Galα4Galβ3Manβ4Glcβ1Cer and (GalNAcα4)1-4 GalNAcα4Galβ3Manβ4Glcβ1Cer.
29069405	6	16	from	patterns	942:949	arg1	larvae					954:959	larvae	954:959	larvae	954:959	GSL expression patterns in larvae and pupae were compared using thin-layer chromatography, which demonstrated differences among acidic, polar and neutral GSL fractions, while the zwitterionic fraction showed no difference.
29069405	6	16	from	patterns	942:949	arg1	pupae					965:969	pupae	965:969	pupae	965:969	GSL expression patterns in larvae and pupae were compared using thin-layer chromatography, which demonstrated differences among acidic, polar and neutral GSL fractions, while the zwitterionic fraction showed no difference.
29069405	8	17	theme	MS	1274:1275	arg1	analysis					1277:1284	MALDI-TOF MS analysis	1264:1284	MALDI-TOF MS analysis	1264:1284	MALDI-TOF MS analysis revealed that larval GSLs contained four types of ceramide species, whereas pupal GSLs contained only two types.
29069405	9	18	theme	structural	1403:1412	arg1	analysis					1414:1421	The structural analysis	1399:1421	The structural analysis of neutral GSLs from silkworms	1399:1452	The structural analysis of neutral GSLs from silkworms revealed a novel series of GSLs.
29069405	3	19	dep	five	686:689	arg1	to					683:684	to	683:684	to	683:684	Lin-ear elongation of α4-GalNAc was observed at the non-reducing end of Galβ3Manβ4Glcβ1Cer with up to five GalNAc repeats.
29069405	8	20	theme	ceramide	1336:1343	arg1	species					1345:1351	ceramide species	1336:1351	ceramide species	1336:1351	MALDI-TOF MS analysis revealed that larval GSLs contained four types of ceramide species, whereas pupal GSLs contained only two types.
29069405	0	21	theme	ceramide	102:109	arg1	composition					111:121	ceramide composition	102:121	ceramide composition	102:121	Structural analysis of neutral glycosphingolipids from the silkworm Bombyx mori and the difference in ceramide composition between larvae and pupae.
29069405	9	22	from	silkworms	1444:1452	arg1	analysis					1414:1421	The structural analysis	1399:1421	The structural analysis of neutral GSLs from silkworms	1399:1452	The structural analysis of neutral GSLs from silkworms revealed a novel series of GSLs.
29069405	9	22	from	silkworms	1444:1452	arg1	GSLs					1434:1437	neutral GSLs	1426:1437	neutral GSLs from silkworms	1426:1452	The structural analysis of neutral GSLs from silkworms revealed a novel series of GSLs.
29069405	10	23	theme	expression	1509:1518	arg1	patterns					1520:1527	GSL expression patterns	1505:1527	GSL expression patterns	1505:1527	The comparison of GSL expression patterns between larvae and pupae demonstrated differences in several fractions.
29069405	3	24	theme	α4-GalNAc	606:614	arg1	elongation					592:601	Lin-ear elongation	584:601	Lin-ear elongation of α4-GalNAc	584:614	Lin-ear elongation of α4-GalNAc was observed at the non-reducing end of Galβ3Manβ4Glcβ1Cer with up to five GalNAc repeats.
29069405	7	25	from	di-	1181:1183	arg1	larvae					1215:1220	larvae	1215:1220	larvae	1215:1220	Neutral GSLs such as ceramides di-, tri- and tetrasaccharides in larvae showed less abundant than those in pupae.
29069405	9	26	from	analysis	1414:1421	arg1	silkworms					1444:1452	silkworms	1444:1452	silkworms	1444:1452	The structural analysis of neutral GSLs from silkworms revealed a novel series of GSLs.
29069405	6	27	theme	polar	1063:1067	arg1	fractions					1085:1093	acidic, polar and neutral GSL fractions	1055:1093	acidic, polar and neutral GSL fractions	1055:1093	GSL expression patterns in larvae and pupae were compared using thin-layer chromatography, which demonstrated differences among acidic, polar and neutral GSL fractions, while the zwitterionic fraction showed no difference.
29069405	9	28	theme	neutral	1426:1432	arg1	GSLs					1434:1437	neutral GSLs	1426:1437	neutral GSLs from silkworms	1426:1452	The structural analysis of neutral GSLs from silkworms revealed a novel series of GSLs.
29069405	2	29	theme	neutral	316:322	arg1	GSLs					324:327	neutral GSLs	316:327	neutral GSLs from dried pupae	316:344	The structural analysis of neutral GSLs from dried pupae revealed the following predominant species: Glcβ1Cer, Manβ4Glcβ1Cer, GlcNAcβ3Manβ4Glcβ1Cer, Galβ3Manβ4Glcβ1Cer, GalNAcα4Galβ3Manβ4Glcβ1Cer, GlcNAcβ3Galβ3Manβ4Glcβ1Cer, Galα4Galβ3Manβ4Glcβ1Cer and (GalNAcα4)1-4 GalNAcα4Galβ3Manβ4Glcβ1Cer.
29069405	4	30	theme	characteristic	754:767	arg1	sequence					780:787	a characteristic GSL-glycan sequence	752:787	a characteristic GSL-glycan sequence of other Arthropoda	752:807	The arthro-series GSL GlcNAcβ3Manβ4Glcβ1Cer, a characteristic GSL-glycan sequence of other Arthropoda, was detected in silkworms.
29069405	4	30	theme	characteristic	754:767	arg1	GlcNAcβ3Manβ4Glcβ1Cer					729:749	The arthro-series GSL GlcNAcβ3Manβ4Glcβ1Cer	707:749	The arthro-series GSL GlcNAcβ3Manβ4Glcβ1Cer	707:749	The arthro-series GSL GlcNAcβ3Manβ4Glcβ1Cer, a characteristic GSL-glycan sequence of other Arthropoda, was detected in silkworms.
29069405	0	31	theme	Structural	0:9	arg1	analysis					11:18	Structural analysis	0:18	Structural analysis of neutral glycosphingolipids from the silkworm Bombyx mori and the difference in ceramide composition between larvae and pupae.	0:147	Structural analysis of neutral glycosphingolipids from the silkworm Bombyx mori and the difference in ceramide composition between larvae and pupae.
29069405	6	32	theme	GSL	1081:1083	arg1	fractions					1085:1093	acidic, polar and neutral GSL fractions	1055:1093	acidic, polar and neutral GSL fractions	1055:1093	GSL expression patterns in larvae and pupae were compared using thin-layer chromatography, which demonstrated differences among acidic, polar and neutral GSL fractions, while the zwitterionic fraction showed no difference.
29069405	11	33	theme	MALDI-TOF	1683:1691	arg1	analysis					1696:1703	MALDI-TOF MS analysis	1683:1703	MALDI-TOF MS analysis	1683:1703	Alterations in GSL ceramide composition between larvae and pupae were observed by MALDI-TOF MS analysis.
29069405	3	34	located	observed	620:627	arg2	elongation					592:601	Lin-ear elongation	584:601	Lin-ear elongation of α4-GalNAc	584:614	Lin-ear elongation of α4-GalNAc was observed at the non-reducing end of Galβ3Manβ4Glcβ1Cer with up to five GalNAc repeats.
29069405	3	34	located	observed	620:627	arg1	end					649:651	the non-reducing end	632:651	the non-reducing end of Galβ3Manβ4Glcβ1Cer with up to five GalNAc repeats	632:704	Lin-ear elongation of α4-GalNAc was observed at the non-reducing end of Galβ3Manβ4Glcβ1Cer with up to five GalNAc repeats.
29069405	1	35	theme	silkworm	184:191	arg1	mori					200:203	the silkworm Bombyx mori	180:203	the silkworm Bombyx mori	180:203	Glycosphingolipids (GSLs) from the silkworm Bombyx mori were identified and GSL expression patterns between larvae and pupae were compared.
29069405	0	36	theme	neutral	23:29	arg1	glycosphingolipids					31:48	neutral glycosphingolipids	23:48	neutral glycosphingolipids from the silkworm Bombyx mori and the difference in ceramide composition between larvae and pupae	23:146	Structural analysis of neutral glycosphingolipids from the silkworm Bombyx mori and the difference in ceramide composition between larvae and pupae.
29069405	6	37	theme	neutral	1073:1079	arg1	fractions					1085:1093	acidic, polar and neutral GSL fractions	1055:1093	acidic, polar and neutral GSL fractions	1055:1093	GSL expression patterns in larvae and pupae were compared using thin-layer chromatography, which demonstrated differences among acidic, polar and neutral GSL fractions, while the zwitterionic fraction showed no difference.
29069405	8	38	contain	contained	1312:1320	arg2	types					1327:1331	four types	1322:1331	four types of ceramide species	1322:1351	MALDI-TOF MS analysis revealed that larval GSLs contained four types of ceramide species, whereas pupal GSLs contained only two types.
29069405	8	38	contain	contained	1312:1320	arg1	GSLs					1307:1310	larval GSLs	1300:1310	larval GSLs	1300:1310	MALDI-TOF MS analysis revealed that larval GSLs contained four types of ceramide species, whereas pupal GSLs contained only two types.
29069405	11	39	from	Alterations	1601:1611	arg1	composition					1629:1639	GSL ceramide composition	1616:1639	GSL ceramide composition between larvae and pupae	1616:1664	Alterations in GSL ceramide composition between larvae and pupae were observed by MALDI-TOF MS analysis.
29069405	9	40	theme	GSLs	1434:1437	arg1	analysis					1414:1421	The structural analysis	1399:1421	The structural analysis of neutral GSLs from silkworms	1399:1452	The structural analysis of neutral GSLs from silkworms revealed a novel series of GSLs.
29069405	1	41	theme	Bombyx	193:198	arg1	mori					200:203	the silkworm Bombyx mori	180:203	the silkworm Bombyx mori	180:203	Glycosphingolipids (GSLs) from the silkworm Bombyx mori were identified and GSL expression patterns between larvae and pupae were compared.
29069405	3	42	with	end	649:651	arg1	repeats					698:704	up to five GalNAc repeats	680:704	up to five GalNAc repeats	680:704	Lin-ear elongation of α4-GalNAc was observed at the non-reducing end of Galβ3Manβ4Glcβ1Cer with up to five GalNAc repeats.
29069405	2	43	theme	structural	293:302	arg1	analysis					304:311	The structural analysis	289:311	The structural analysis of neutral GSLs from dried pupae	289:344	The structural analysis of neutral GSLs from dried pupae revealed the following predominant species: Glcβ1Cer, Manβ4Glcβ1Cer, GlcNAcβ3Manβ4Glcβ1Cer, Galβ3Manβ4Glcβ1Cer, GalNAcα4Galβ3Manβ4Glcβ1Cer, GlcNAcβ3Galβ3Manβ4Glcβ1Cer, Galα4Galβ3Manβ4Glcβ1Cer and (GalNAcα4)1-4 GalNAcα4Galβ3Manβ4Glcβ1Cer.
29069405	3	44	theme	Lin-ear	584:590	arg1	elongation					592:601	Lin-ear elongation	584:601	Lin-ear elongation of α4-GalNAc	584:614	Lin-ear elongation of α4-GalNAc was observed at the non-reducing end of Galβ3Manβ4Glcβ1Cer with up to five GalNAc repeats.
29069405	4	45	theme	Arthropoda	798:807	arg1	sequence					780:787	a characteristic GSL-glycan sequence	752:787	a characteristic GSL-glycan sequence of other Arthropoda	752:807	The arthro-series GSL GlcNAcβ3Manβ4Glcβ1Cer, a characteristic GSL-glycan sequence of other Arthropoda, was detected in silkworms.
29069405	4	45	theme	Arthropoda	798:807	arg1	GlcNAcβ3Manβ4Glcβ1Cer					729:749	The arthro-series GSL GlcNAcβ3Manβ4Glcβ1Cer	707:749	The arthro-series GSL GlcNAcβ3Manβ4Glcβ1Cer	707:749	The arthro-series GSL GlcNAcβ3Manβ4Glcβ1Cer, a characteristic GSL-glycan sequence of other Arthropoda, was detected in silkworms.
29069405	8	46	dep	contained	1312:1320	arg1	whereas					1354:1360	whereas	1354:1360	whereas	1354:1360	MALDI-TOF MS analysis revealed that larval GSLs contained four types of ceramide species, whereas pupal GSLs contained only two types.
29069405	0	47	dep	silkworm	59:66	arg1	mori					75:78	Bombyx mori	68:78	the silkworm Bombyx mori	55:78	Structural analysis of neutral glycosphingolipids from the silkworm Bombyx mori and the difference in ceramide composition between larvae and pupae.
29069405	8	48	theme	species	1345:1351	arg1	types					1327:1331	four types	1322:1331	four types of ceramide species	1322:1351	MALDI-TOF MS analysis revealed that larval GSLs contained four types of ceramide species, whereas pupal GSLs contained only two types.
29069405	4	49	theme	GSL	725:727	arg1	sequence					780:787	a characteristic GSL-glycan sequence	752:787	a characteristic GSL-glycan sequence of other Arthropoda	752:807	The arthro-series GSL GlcNAcβ3Manβ4Glcβ1Cer, a characteristic GSL-glycan sequence of other Arthropoda, was detected in silkworms.
29069405	4	49	theme	GSL	725:727	arg1	GlcNAcβ3Manβ4Glcβ1Cer					729:749	The arthro-series GSL GlcNAcβ3Manβ4Glcβ1Cer	707:749	The arthro-series GSL GlcNAcβ3Manβ4Glcβ1Cer	707:749	The arthro-series GSL GlcNAcβ3Manβ4Glcβ1Cer, a characteristic GSL-glycan sequence of other Arthropoda, was detected in silkworms.
29069405	0	50	theme	glycosphingolipids	31:48	arg1	analysis					11:18	Structural analysis	0:18	Structural analysis of neutral glycosphingolipids from the silkworm Bombyx mori and the difference in ceramide composition between larvae and pupae.	0:147	Structural analysis of neutral glycosphingolipids from the silkworm Bombyx mori and the difference in ceramide composition between larvae and pupae.
29069405	5	51	theme	purified	871:878	arg1	fraction					884:891	each purified GSL fraction	866:891	each purified GSL fraction	866:891	The main ceramide species in each purified GSL fraction were h20:0-d14:1 and h22:0-d14:1.
29069405	2	52	dep	Manβ4Glcβ1Cer	400:412	arg1	GalNAcα4Galβ3Manβ4Glcβ1Cer					556:581	GalNAcα4Galβ3Manβ4Glcβ1Cer	556:581	GalNAcα4Galβ3Manβ4Glcβ1Cer	556:581	The structural analysis of neutral GSLs from dried pupae revealed the following predominant species: Glcβ1Cer, Manβ4Glcβ1Cer, GlcNAcβ3Manβ4Glcβ1Cer, Galβ3Manβ4Glcβ1Cer, GalNAcα4Galβ3Manβ4Glcβ1Cer, GlcNAcβ3Galβ3Manβ4Glcβ1Cer, Galα4Galβ3Manβ4Glcβ1Cer and (GalNAcα4)1-4 GalNAcα4Galβ3Manβ4Glcβ1Cer.
29069405	4	53	theme	arthro-series	711:723	arg1	sequence					780:787	a characteristic GSL-glycan sequence	752:787	a characteristic GSL-glycan sequence of other Arthropoda	752:807	The arthro-series GSL GlcNAcβ3Manβ4Glcβ1Cer, a characteristic GSL-glycan sequence of other Arthropoda, was detected in silkworms.
29069405	4	53	theme	arthro-series	711:723	arg1	GlcNAcβ3Manβ4Glcβ1Cer					729:749	The arthro-series GSL GlcNAcβ3Manβ4Glcβ1Cer	707:749	The arthro-series GSL GlcNAcβ3Manβ4Glcβ1Cer	707:749	The arthro-series GSL GlcNAcβ3Manβ4Glcβ1Cer, a characteristic GSL-glycan sequence of other Arthropoda, was detected in silkworms.
29069405	5	54	theme	GSL	880:882	arg1	fraction					884:891	each purified GSL fraction	866:891	each purified GSL fraction	866:891	The main ceramide species in each purified GSL fraction were h20:0-d14:1 and h22:0-d14:1.
29069405	6	55	theme	acidic	1055:1060	arg1	fractions					1085:1093	acidic, polar and neutral GSL fractions	1055:1093	acidic, polar and neutral GSL fractions	1055:1093	GSL expression patterns in larvae and pupae were compared using thin-layer chromatography, which demonstrated differences among acidic, polar and neutral GSL fractions, while the zwitterionic fraction showed no difference.
29069405	7	56	theme	Neutral	1150:1156	arg1	GSLs					1158:1161	Neutral GSLs	1150:1161	Neutral GSLs such as ceramides di-, tri- and tetrasaccharides in larvae	1150:1220	Neutral GSLs such as ceramides di-, tri- and tetrasaccharides in larvae showed less abundant than those in pupae.
29069405	7	56	theme	Neutral	1150:1156	arg1	ceramides					1171:1179	ceramides di-, tri- and tetrasaccharides in larvae	1171:1220	ceramides di-, tri- and tetrasaccharides in larvae	1171:1220	Neutral GSLs such as ceramides di-, tri- and tetrasaccharides in larvae showed less abundant than those in pupae.
29069405	5	57	theme	ceramide	846:853	arg1	species					855:861	The main ceramide species	837:861	The main ceramide species in each purified GSL fraction	837:891	The main ceramide species in each purified GSL fraction were h20:0-d14:1 and h22:0-d14:1.
29069405	10	58	theme	several	1582:1588	arg1	fractions					1590:1598	several fractions	1582:1598	several fractions	1582:1598	The comparison of GSL expression patterns between larvae and pupae demonstrated differences in several fractions.
29069405	9	59	theme	novel	1465:1469	arg1	series					1471:1476	a novel series	1463:1476	a novel series of GSLs	1463:1484	The structural analysis of neutral GSLs from silkworms revealed a novel series of GSLs.
29069405	5	60	from	species	855:861	arg1	fraction					884:891	each purified GSL fraction	866:891	each purified GSL fraction	866:891	The main ceramide species in each purified GSL fraction were h20:0-d14:1 and h22:0-d14:1.
29069405	3	61	theme	Galβ3Manβ4Glcβ1Cer	656:673	arg1	end					649:651	the non-reducing end	632:651	the non-reducing end of Galβ3Manβ4Glcβ1Cer with up to five GalNAc repeats	632:704	Lin-ear elongation of α4-GalNAc was observed at the non-reducing end of Galβ3Manβ4Glcβ1Cer with up to five GalNAc repeats.
29069405	11	62	theme	MS	1693:1694	arg1	analysis					1696:1703	MALDI-TOF MS analysis	1683:1703	MALDI-TOF MS analysis	1683:1703	Alterations in GSL ceramide composition between larvae and pupae were observed by MALDI-TOF MS analysis.
29069405	2	63	theme	dried	334:338	arg1	pupae					340:344	dried pupae	334:344	dried pupae	334:344	The structural analysis of neutral GSLs from dried pupae revealed the following predominant species: Glcβ1Cer, Manβ4Glcβ1Cer, GlcNAcβ3Manβ4Glcβ1Cer, Galβ3Manβ4Glcβ1Cer, GalNAcα4Galβ3Manβ4Glcβ1Cer, GlcNAcβ3Galβ3Manβ4Glcβ1Cer, Galα4Galβ3Manβ4Glcβ1Cer and (GalNAcα4)1-4 GalNAcα4Galβ3Manβ4Glcβ1Cer.
29069405	10	64	from	differences	1567:1577	arg1	fractions					1590:1598	several fractions	1582:1598	several fractions	1582:1598	The comparison of GSL expression patterns between larvae and pupae demonstrated differences in several fractions.
29069405	6	65	theme	expression	931:940	arg1	patterns					942:949	GSL expression patterns	927:949	GSL expression patterns in larvae and pupae	927:969	GSL expression patterns in larvae and pupae were compared using thin-layer chromatography, which demonstrated differences among acidic, polar and neutral GSL fractions, while the zwitterionic fraction showed no difference.
29069405	3	66	theme	GalNAc	691:696	arg1	repeats					698:704	up to five GalNAc repeats	680:704	up to five GalNAc repeats	680:704	Lin-ear elongation of α4-GalNAc was observed at the non-reducing end of Galβ3Manβ4Glcβ1Cer with up to five GalNAc repeats.
29069405	1	67	from	mori	200:203	arg1	Glycosphingolipids					149:166	Glycosphingolipids	149:166	Glycosphingolipids (GSLs) from the silkworm Bombyx mori	149:203	Glycosphingolipids (GSLs) from the silkworm Bombyx mori were identified and GSL expression patterns between larvae and pupae were compared.
29069405	1	67	from	mori	200:203	arg1	GSLs					169:172	GSLs	169:172	GSLs	169:172	Glycosphingolipids (GSLs) from the silkworm Bombyx mori were identified and GSL expression patterns between larvae and pupae were compared.
29069405	6	68	theme	GSL	927:929	arg1	patterns					942:949	GSL expression patterns	927:949	GSL expression patterns in larvae and pupae	927:969	GSL expression patterns in larvae and pupae were compared using thin-layer chromatography, which demonstrated differences among acidic, polar and neutral GSL fractions, while the zwitterionic fraction showed no difference.
29069405	2	69	dep	species	381:387	arg1	Galβ3Manβ4Glcβ1Cer					438:455	Galβ3Manβ4Glcβ1Cer	438:455	Galβ3Manβ4Glcβ1Cer	438:455	The structural analysis of neutral GSLs from dried pupae revealed the following predominant species: Glcβ1Cer, Manβ4Glcβ1Cer, GlcNAcβ3Manβ4Glcβ1Cer, Galβ3Manβ4Glcβ1Cer, GalNAcα4Galβ3Manβ4Glcβ1Cer, GlcNAcβ3Galβ3Manβ4Glcβ1Cer, Galα4Galβ3Manβ4Glcβ1Cer and (GalNAcα4)1-4 GalNAcα4Galβ3Manβ4Glcβ1Cer.
29069405	2	69	dep	species	381:387	arg1	GalNAcα4					543:550	GalNAcα4	543:550	GalNAcα4	543:550	The structural analysis of neutral GSLs from dried pupae revealed the following predominant species: Glcβ1Cer, Manβ4Glcβ1Cer, GlcNAcβ3Manβ4Glcβ1Cer, Galβ3Manβ4Glcβ1Cer, GalNAcα4Galβ3Manβ4Glcβ1Cer, GlcNAcβ3Galβ3Manβ4Glcβ1Cer, Galα4Galβ3Manβ4Glcβ1Cer and (GalNAcα4)1-4 GalNAcα4Galβ3Manβ4Glcβ1Cer.
29069405	2	69	dep	species	381:387	arg1	Manβ4Glcβ1Cer					400:412	Manβ4Glcβ1Cer	400:412	Manβ4Glcβ1Cer	400:412	The structural analysis of neutral GSLs from dried pupae revealed the following predominant species: Glcβ1Cer, Manβ4Glcβ1Cer, GlcNAcβ3Manβ4Glcβ1Cer, Galβ3Manβ4Glcβ1Cer, GalNAcα4Galβ3Manβ4Glcβ1Cer, GlcNAcβ3Galβ3Manβ4Glcβ1Cer, Galα4Galβ3Manβ4Glcβ1Cer and (GalNAcα4)1-4 GalNAcα4Galβ3Manβ4Glcβ1Cer.
29069405	2	69	dep	species	381:387	arg1	GlcNAcβ3Galβ3Manβ4Glcβ1Cer					486:511	GlcNAcβ3Galβ3Manβ4Glcβ1Cer	486:511	GlcNAcβ3Galβ3Manβ4Glcβ1Cer	486:511	The structural analysis of neutral GSLs from dried pupae revealed the following predominant species: Glcβ1Cer, Manβ4Glcβ1Cer, GlcNAcβ3Manβ4Glcβ1Cer, Galβ3Manβ4Glcβ1Cer, GalNAcα4Galβ3Manβ4Glcβ1Cer, GlcNAcβ3Galβ3Manβ4Glcβ1Cer, Galα4Galβ3Manβ4Glcβ1Cer and (GalNAcα4)1-4 GalNAcα4Galβ3Manβ4Glcβ1Cer.
29069405	2	69	dep	species	381:387	arg1	species					381:387	the following predominant species	355:387	the following predominant species: Glcβ1Cer, Manβ4Glcβ1Cer, GlcNAcβ3Manβ4Glcβ1Cer, Galβ3Manβ4Glcβ1Cer, GalNAcα4Galβ3Manβ4Glcβ1Cer, GlcNAcβ3Galβ3Manβ4Glcβ1Cer, Galα4Galβ3Manβ4Glcβ1Cer and (GalNAcα4)1-4 GalNAcα4Galβ3Manβ4Glcβ1Cer	355:581	The structural analysis of neutral GSLs from dried pupae revealed the following predominant species: Glcβ1Cer, Manβ4Glcβ1Cer, GlcNAcβ3Manβ4Glcβ1Cer, Galβ3Manβ4Glcβ1Cer, GalNAcα4Galβ3Manβ4Glcβ1Cer, GlcNAcβ3Galβ3Manβ4Glcβ1Cer, Galα4Galβ3Manβ4Glcβ1Cer and (GalNAcα4)1-4 GalNAcα4Galβ3Manβ4Glcβ1Cer.
29069405	2	69	dep	species	381:387	arg1	Galα4Galβ3Manβ4Glcβ1Cer					514:536	Galα4Galβ3Manβ4Glcβ1Cer	514:536	Galα4Galβ3Manβ4Glcβ1Cer	514:536	The structural analysis of neutral GSLs from dried pupae revealed the following predominant species: Glcβ1Cer, Manβ4Glcβ1Cer, GlcNAcβ3Manβ4Glcβ1Cer, Galβ3Manβ4Glcβ1Cer, GalNAcα4Galβ3Manβ4Glcβ1Cer, GlcNAcβ3Galβ3Manβ4Glcβ1Cer, Galα4Galβ3Manβ4Glcβ1Cer and (GalNAcα4)1-4 GalNAcα4Galβ3Manβ4Glcβ1Cer.
29069405	7	70	from	tetrasaccharides	1195:1210	arg1	larvae					1215:1220	larvae	1215:1220	larvae	1215:1220	Neutral GSLs such as ceramides di-, tri- and tetrasaccharides in larvae showed less abundant than those in pupae.
29069405	2	71	theme	GSLs	324:327	arg1	analysis					304:311	The structural analysis	289:311	The structural analysis of neutral GSLs from dried pupae	289:344	The structural analysis of neutral GSLs from dried pupae revealed the following predominant species: Glcβ1Cer, Manβ4Glcβ1Cer, GlcNAcβ3Manβ4Glcβ1Cer, Galβ3Manβ4Glcβ1Cer, GalNAcα4Galβ3Manβ4Glcβ1Cer, GlcNAcβ3Galβ3Manβ4Glcβ1Cer, Galα4Galβ3Manβ4Glcβ1Cer and (GalNAcα4)1-4 GalNAcα4Galβ3Manβ4Glcβ1Cer.
29069405	11	72	theme	ceramide	1620:1627	arg1	composition					1629:1639	GSL ceramide composition	1616:1639	GSL ceramide composition between larvae and pupae	1616:1664	Alterations in GSL ceramide composition between larvae and pupae were observed by MALDI-TOF MS analysis.
29069405	1	73	theme	GSL	225:227	arg1	patterns					240:247	GSL expression patterns	225:247	GSL expression patterns between larvae and pupae	225:272	Glycosphingolipids (GSLs) from the silkworm Bombyx mori were identified and GSL expression patterns between larvae and pupae were compared.
29069405	5	74	theme	main	841:844	arg1	species					855:861	The main ceramide species	837:861	The main ceramide species in each purified GSL fraction	837:891	The main ceramide species in each purified GSL fraction were h20:0-d14:1 and h22:0-d14:1.
29069405	0	75	from	difference	88:97	arg1	analysis					11:18	Structural analysis	0:18	Structural analysis of neutral glycosphingolipids from the silkworm Bombyx mori and the difference in ceramide composition between larvae and pupae.	0:147	Structural analysis of neutral glycosphingolipids from the silkworm Bombyx mori and the difference in ceramide composition between larvae and pupae.
29069405	0	75	from	difference	88:97	arg1	glycosphingolipids					31:48	neutral glycosphingolipids	23:48	neutral glycosphingolipids from the silkworm Bombyx mori and the difference in ceramide composition between larvae and pupae	23:146	Structural analysis of neutral glycosphingolipids from the silkworm Bombyx mori and the difference in ceramide composition between larvae and pupae.
29069405	0	75	from	difference	88:97	arg1	composition					111:121	ceramide composition	102:121	ceramide composition	102:121	Structural analysis of neutral glycosphingolipids from the silkworm Bombyx mori and the difference in ceramide composition between larvae and pupae.
29069405	8	76	theme	pupal	1362:1366	arg1	GSLs					1368:1371	pupal GSLs	1362:1371	pupal GSLs	1362:1371	MALDI-TOF MS analysis revealed that larval GSLs contained four types of ceramide species, whereas pupal GSLs contained only two types.
29069405	2	77	theme	predominant	369:379	arg1	Galβ3Manβ4Glcβ1Cer					438:455	Galβ3Manβ4Glcβ1Cer	438:455	Galβ3Manβ4Glcβ1Cer	438:455	The structural analysis of neutral GSLs from dried pupae revealed the following predominant species: Glcβ1Cer, Manβ4Glcβ1Cer, GlcNAcβ3Manβ4Glcβ1Cer, Galβ3Manβ4Glcβ1Cer, GalNAcα4Galβ3Manβ4Glcβ1Cer, GlcNAcβ3Galβ3Manβ4Glcβ1Cer, Galα4Galβ3Manβ4Glcβ1Cer and (GalNAcα4)1-4 GalNAcα4Galβ3Manβ4Glcβ1Cer.
29069405	2	77	theme	predominant	369:379	arg1	GalNAcα4					543:550	GalNAcα4	543:550	GalNAcα4	543:550	The structural analysis of neutral GSLs from dried pupae revealed the following predominant species: Glcβ1Cer, Manβ4Glcβ1Cer, GlcNAcβ3Manβ4Glcβ1Cer, Galβ3Manβ4Glcβ1Cer, GalNAcα4Galβ3Manβ4Glcβ1Cer, GlcNAcβ3Galβ3Manβ4Glcβ1Cer, Galα4Galβ3Manβ4Glcβ1Cer and (GalNAcα4)1-4 GalNAcα4Galβ3Manβ4Glcβ1Cer.
29069405	2	77	theme	predominant	369:379	arg1	Manβ4Glcβ1Cer					400:412	Manβ4Glcβ1Cer	400:412	Manβ4Glcβ1Cer	400:412	The structural analysis of neutral GSLs from dried pupae revealed the following predominant species: Glcβ1Cer, Manβ4Glcβ1Cer, GlcNAcβ3Manβ4Glcβ1Cer, Galβ3Manβ4Glcβ1Cer, GalNAcα4Galβ3Manβ4Glcβ1Cer, GlcNAcβ3Galβ3Manβ4Glcβ1Cer, Galα4Galβ3Manβ4Glcβ1Cer and (GalNAcα4)1-4 GalNAcα4Galβ3Manβ4Glcβ1Cer.
29069405	2	77	theme	predominant	369:379	arg1	GlcNAcβ3Galβ3Manβ4Glcβ1Cer					486:511	GlcNAcβ3Galβ3Manβ4Glcβ1Cer	486:511	GlcNAcβ3Galβ3Manβ4Glcβ1Cer	486:511	The structural analysis of neutral GSLs from dried pupae revealed the following predominant species: Glcβ1Cer, Manβ4Glcβ1Cer, GlcNAcβ3Manβ4Glcβ1Cer, Galβ3Manβ4Glcβ1Cer, GalNAcα4Galβ3Manβ4Glcβ1Cer, GlcNAcβ3Galβ3Manβ4Glcβ1Cer, Galα4Galβ3Manβ4Glcβ1Cer and (GalNAcα4)1-4 GalNAcα4Galβ3Manβ4Glcβ1Cer.
29069405	2	77	theme	predominant	369:379	arg1	species					381:387	the following predominant species	355:387	the following predominant species: Glcβ1Cer, Manβ4Glcβ1Cer, GlcNAcβ3Manβ4Glcβ1Cer, Galβ3Manβ4Glcβ1Cer, GalNAcα4Galβ3Manβ4Glcβ1Cer, GlcNAcβ3Galβ3Manβ4Glcβ1Cer, Galα4Galβ3Manβ4Glcβ1Cer and (GalNAcα4)1-4 GalNAcα4Galβ3Manβ4Glcβ1Cer	355:581	The structural analysis of neutral GSLs from dried pupae revealed the following predominant species: Glcβ1Cer, Manβ4Glcβ1Cer, GlcNAcβ3Manβ4Glcβ1Cer, Galβ3Manβ4Glcβ1Cer, GalNAcα4Galβ3Manβ4Glcβ1Cer, GlcNAcβ3Galβ3Manβ4Glcβ1Cer, Galα4Galβ3Manβ4Glcβ1Cer and (GalNAcα4)1-4 GalNAcα4Galβ3Manβ4Glcβ1Cer.
29069405	2	77	theme	predominant	369:379	arg1	Galα4Galβ3Manβ4Glcβ1Cer					514:536	Galα4Galβ3Manβ4Glcβ1Cer	514:536	Galα4Galβ3Manβ4Glcβ1Cer	514:536	The structural analysis of neutral GSLs from dried pupae revealed the following predominant species: Glcβ1Cer, Manβ4Glcβ1Cer, GlcNAcβ3Manβ4Glcβ1Cer, Galβ3Manβ4Glcβ1Cer, GalNAcα4Galβ3Manβ4Glcβ1Cer, GlcNAcβ3Galβ3Manβ4Glcβ1Cer, Galα4Galβ3Manβ4Glcβ1Cer and (GalNAcα4)1-4 GalNAcα4Galβ3Manβ4Glcβ1Cer.
29069405	1	78	theme	expression	229:238	arg1	patterns					240:247	GSL expression patterns	225:247	GSL expression patterns between larvae and pupae	225:272	Glycosphingolipids (GSLs) from the silkworm Bombyx mori were identified and GSL expression patterns between larvae and pupae were compared.
29069405	0	79	theme	Bombyx	68:73	arg1	mori					75:78	Bombyx mori	68:78	the silkworm Bombyx mori	55:78	Structural analysis of neutral glycosphingolipids from the silkworm Bombyx mori and the difference in ceramide composition between larvae and pupae.
29069405	10	80	theme	GSL	1505:1507	arg1	patterns					1520:1527	GSL expression patterns	1505:1527	GSL expression patterns	1505:1527	The comparison of GSL expression patterns between larvae and pupae demonstrated differences in several fractions.
29069405	2	81	theme	following	359:367	arg1	Galβ3Manβ4Glcβ1Cer					438:455	Galβ3Manβ4Glcβ1Cer	438:455	Galβ3Manβ4Glcβ1Cer	438:455	The structural analysis of neutral GSLs from dried pupae revealed the following predominant species: Glcβ1Cer, Manβ4Glcβ1Cer, GlcNAcβ3Manβ4Glcβ1Cer, Galβ3Manβ4Glcβ1Cer, GalNAcα4Galβ3Manβ4Glcβ1Cer, GlcNAcβ3Galβ3Manβ4Glcβ1Cer, Galα4Galβ3Manβ4Glcβ1Cer and (GalNAcα4)1-4 GalNAcα4Galβ3Manβ4Glcβ1Cer.
29069405	2	81	theme	following	359:367	arg1	GalNAcα4					543:550	GalNAcα4	543:550	GalNAcα4	543:550	The structural analysis of neutral GSLs from dried pupae revealed the following predominant species: Glcβ1Cer, Manβ4Glcβ1Cer, GlcNAcβ3Manβ4Glcβ1Cer, Galβ3Manβ4Glcβ1Cer, GalNAcα4Galβ3Manβ4Glcβ1Cer, GlcNAcβ3Galβ3Manβ4Glcβ1Cer, Galα4Galβ3Manβ4Glcβ1Cer and (GalNAcα4)1-4 GalNAcα4Galβ3Manβ4Glcβ1Cer.
29069405	2	81	theme	following	359:367	arg1	Manβ4Glcβ1Cer					400:412	Manβ4Glcβ1Cer	400:412	Manβ4Glcβ1Cer	400:412	The structural analysis of neutral GSLs from dried pupae revealed the following predominant species: Glcβ1Cer, Manβ4Glcβ1Cer, GlcNAcβ3Manβ4Glcβ1Cer, Galβ3Manβ4Glcβ1Cer, GalNAcα4Galβ3Manβ4Glcβ1Cer, GlcNAcβ3Galβ3Manβ4Glcβ1Cer, Galα4Galβ3Manβ4Glcβ1Cer and (GalNAcα4)1-4 GalNAcα4Galβ3Manβ4Glcβ1Cer.
29069405	2	81	theme	following	359:367	arg1	GlcNAcβ3Galβ3Manβ4Glcβ1Cer					486:511	GlcNAcβ3Galβ3Manβ4Glcβ1Cer	486:511	GlcNAcβ3Galβ3Manβ4Glcβ1Cer	486:511	The structural analysis of neutral GSLs from dried pupae revealed the following predominant species: Glcβ1Cer, Manβ4Glcβ1Cer, GlcNAcβ3Manβ4Glcβ1Cer, Galβ3Manβ4Glcβ1Cer, GalNAcα4Galβ3Manβ4Glcβ1Cer, GlcNAcβ3Galβ3Manβ4Glcβ1Cer, Galα4Galβ3Manβ4Glcβ1Cer and (GalNAcα4)1-4 GalNAcα4Galβ3Manβ4Glcβ1Cer.
29069405	2	81	theme	following	359:367	arg1	species					381:387	the following predominant species	355:387	the following predominant species: Glcβ1Cer, Manβ4Glcβ1Cer, GlcNAcβ3Manβ4Glcβ1Cer, Galβ3Manβ4Glcβ1Cer, GalNAcα4Galβ3Manβ4Glcβ1Cer, GlcNAcβ3Galβ3Manβ4Glcβ1Cer, Galα4Galβ3Manβ4Glcβ1Cer and (GalNAcα4)1-4 GalNAcα4Galβ3Manβ4Glcβ1Cer	355:581	The structural analysis of neutral GSLs from dried pupae revealed the following predominant species: Glcβ1Cer, Manβ4Glcβ1Cer, GlcNAcβ3Manβ4Glcβ1Cer, Galβ3Manβ4Glcβ1Cer, GalNAcα4Galβ3Manβ4Glcβ1Cer, GlcNAcβ3Galβ3Manβ4Glcβ1Cer, Galα4Galβ3Manβ4Glcβ1Cer and (GalNAcα4)1-4 GalNAcα4Galβ3Manβ4Glcβ1Cer.
29069405	2	81	theme	following	359:367	arg1	Galα4Galβ3Manβ4Glcβ1Cer					514:536	Galα4Galβ3Manβ4Glcβ1Cer	514:536	Galα4Galβ3Manβ4Glcβ1Cer	514:536	The structural analysis of neutral GSLs from dried pupae revealed the following predominant species: Glcβ1Cer, Manβ4Glcβ1Cer, GlcNAcβ3Manβ4Glcβ1Cer, Galβ3Manβ4Glcβ1Cer, GalNAcα4Galβ3Manβ4Glcβ1Cer, GlcNAcβ3Galβ3Manβ4Glcβ1Cer, Galα4Galβ3Manβ4Glcβ1Cer and (GalNAcα4)1-4 GalNAcα4Galβ3Manβ4Glcβ1Cer.
29069405	2	82	from	analysis	304:311	arg1	pupae					340:344	dried pupae	334:344	dried pupae	334:344	The structural analysis of neutral GSLs from dried pupae revealed the following predominant species: Glcβ1Cer, Manβ4Glcβ1Cer, GlcNAcβ3Manβ4Glcβ1Cer, Galβ3Manβ4Glcβ1Cer, GalNAcα4Galβ3Manβ4Glcβ1Cer, GlcNAcβ3Galβ3Manβ4Glcβ1Cer, Galα4Galβ3Manβ4Glcβ1Cer and (GalNAcα4)1-4 GalNAcα4Galβ3Manβ4Glcβ1Cer.
29069405	6	83	theme	zwitterionic	1106:1117	arg1	fraction					1119:1126	the zwitterionic fraction	1102:1126	the zwitterionic fraction	1102:1126	GSL expression patterns in larvae and pupae were compared using thin-layer chromatography, which demonstrated differences among acidic, polar and neutral GSL fractions, while the zwitterionic fraction showed no difference.
31281620	0	0	theme	natural	87:93	arg1	accessions					95:104	Arabidopsis natural accessions	75:104	Arabidopsis natural accessions	75:104	Composition and physicochemical properties of outer mucilage from seeds of Arabidopsis natural accessions.
31281620	1	1	from	layers	181:186	arg1	imbibition					203:212	imbibition	203:212	imbibition	203:212	Arabidopsis thaliana (Arabidopsis) seeds are myxospermous and release two layers of mucilage on imbibition.
31281620	0	2	theme	Arabidopsis	75:85	arg1	accessions					95:104	Arabidopsis natural accessions	75:104	Arabidopsis natural accessions	75:104	Composition and physicochemical properties of outer mucilage from seeds of Arabidopsis natural accessions.
31281620	3	3	theme	outer	362:366	arg1	mucilage					368:375	outer mucilage	362:375	outer mucilage	362:375	The composition and properties of outer mucilage have been determined for 306 natural accessions and six control genotypes to generate a data set comprising six traits measured in four biological replicates for each.
31281620	0	4	from	Composition	0:10	arg1	seeds					66:70	seeds	66:70	seeds of Arabidopsis natural accessions	66:104	Composition and physicochemical properties of outer mucilage from seeds of Arabidopsis natural accessions.
31281620	1	5	theme	thaliana	119:126	arg1	seeds					142:146	Arabidopsis thaliana (Arabidopsis) seeds	107:146	Arabidopsis thaliana (Arabidopsis) seeds	107:146	Arabidopsis thaliana (Arabidopsis) seeds are myxospermous and release two layers of mucilage on imbibition.
31281620	3	6	theme	mucilage	368:375	arg1	composition					332:342	composition	332:342	composition	332:342	The composition and properties of outer mucilage have been determined for 306 natural accessions and six control genotypes to generate a data set comprising six traits measured in four biological replicates for each.
31281620	3	6	theme	mucilage	368:375	arg1	properties					348:357	properties	348:357	properties	348:357	The composition and properties of outer mucilage have been determined for 306 natural accessions and six control genotypes to generate a data set comprising six traits measured in four biological replicates for each.
31281620	4	7	from	possible	581:588	arg1	range					595:599	a range	593:599	a range of analyses	593:611	Future exploitation of this data is possible in a range of analyses and should yield information concerning genetic diversity, underlying genetic factors and the biological function of mucilage as an adaptive trait.
31281620	0	8	theme	mucilage	52:59	arg1	Composition					0:10	Composition	0:10	Composition	0:10	Composition and physicochemical properties of outer mucilage from seeds of Arabidopsis natural accessions.
31281620	0	8	theme	mucilage	52:59	arg1	properties					32:41	physicochemical properties	16:41	physicochemical properties	16:41	Composition and physicochemical properties of outer mucilage from seeds of Arabidopsis natural accessions.
31281620	4	9	theme	genetic	653:659	arg1	diversity					661:669	genetic diversity	653:669	genetic diversity	653:669	Future exploitation of this data is possible in a range of analyses and should yield information concerning genetic diversity, underlying genetic factors and the biological function of mucilage as an adaptive trait.
31281620	4	10	from	range	595:599	arg1	possible					581:588	possible	581:588	possible	581:588	Future exploitation of this data is possible in a range of analyses and should yield information concerning genetic diversity, underlying genetic factors and the biological function of mucilage as an adaptive trait.
31281620	4	11	theme	underlying	672:681	arg1	factors					691:697	underlying genetic factors	672:697	underlying genetic factors	672:697	Future exploitation of this data is possible in a range of analyses and should yield information concerning genetic diversity, underlying genetic factors and the biological function of mucilage as an adaptive trait.
31281620	3	12	theme	control	433:439	arg1	genotypes					441:449	six control genotypes	429:449	six control genotypes	429:449	The composition and properties of outer mucilage have been determined for 306 natural accessions and six control genotypes to generate a data set comprising six traits measured in four biological replicates for each.
31281620	2	13	theme	constituent	298:308	arg1	analysis					276:283	the analysis	272:283	the analysis of its major constituent, polysaccharides	272:325	The outer layer can be extracted with water facilitating the analysis of its major constituent, polysaccharides.
31281620	3	14	theme	data	465:468	arg1	set					470:472	a data set	463:472	a data set comprising six traits measured in four biological replicates for each	463:542	The composition and properties of outer mucilage have been determined for 306 natural accessions and six control genotypes to generate a data set comprising six traits measured in four biological replicates for each.
31281620	0	15	theme	physicochemical	16:30	arg1	properties					32:41	physicochemical properties	16:41	physicochemical properties	16:41	Composition and physicochemical properties of outer mucilage from seeds of Arabidopsis natural accessions.
31281620	4	16	theme	data	573:576	arg1	exploitation					552:563	Future exploitation	545:563	Future exploitation of this data	545:576	Future exploitation of this data is possible in a range of analyses and should yield information concerning genetic diversity, underlying genetic factors and the biological function of mucilage as an adaptive trait.
31281620	2	17	theme	major	292:296	arg1	constituent					298:308	its major constituent	288:308	its major constituent	288:308	The outer layer can be extracted with water facilitating the analysis of its major constituent, polysaccharides.
31281620	2	17	theme	major	292:296	arg1	polysaccharides					311:325	polysaccharides	311:325	polysaccharides	311:325	The outer layer can be extracted with water facilitating the analysis of its major constituent, polysaccharides.
31281620	0	18	theme	outer	46:50	arg1	mucilage					52:59	outer mucilage	46:59	outer mucilage from seeds of Arabidopsis natural accessions	46:104	Composition and physicochemical properties of outer mucilage from seeds of Arabidopsis natural accessions.
31281620	4	19	theme	biological	707:716	arg1	function					718:725	the biological function	703:725	the biological function of mucilage	703:737	Future exploitation of this data is possible in a range of analyses and should yield information concerning genetic diversity, underlying genetic factors and the biological function of mucilage as an adaptive trait.
31281620	3	20	dep	composition	332:342	arg1	The					328:330	The	328:330	The	328:330	The composition and properties of outer mucilage have been determined for 306 natural accessions and six control genotypes to generate a data set comprising six traits measured in four biological replicates for each.
31281620	1	21	theme	mucilage	191:198	arg1	layers					181:186	two layers	177:186	two layers of mucilage on imbibition	177:212	Arabidopsis thaliana (Arabidopsis) seeds are myxospermous and release two layers of mucilage on imbibition.
31281620	0	22	from	seeds	66:70	arg1	Composition					0:10	Composition	0:10	Composition	0:10	Composition and physicochemical properties of outer mucilage from seeds of Arabidopsis natural accessions.
31281620	0	22	from	seeds	66:70	arg1	properties					32:41	physicochemical properties	16:41	physicochemical properties	16:41	Composition and physicochemical properties of outer mucilage from seeds of Arabidopsis natural accessions.
31281620	0	22	from	seeds	66:70	arg1	mucilage					52:59	outer mucilage	46:59	outer mucilage from seeds of Arabidopsis natural accessions	46:104	Composition and physicochemical properties of outer mucilage from seeds of Arabidopsis natural accessions.
31281620	4	23	theme	analyses	604:611	arg1	range					595:599	a range	593:599	a range of analyses	593:611	Future exploitation of this data is possible in a range of analyses and should yield information concerning genetic diversity, underlying genetic factors and the biological function of mucilage as an adaptive trait.
31281620	3	24	theme	natural	406:412	arg1	accessions					414:423	306 natural accessions	402:423	306 natural accessions	402:423	The composition and properties of outer mucilage have been determined for 306 natural accessions and six control genotypes to generate a data set comprising six traits measured in four biological replicates for each.
31281620	4	25	theme	Future	545:550	arg1	exploitation					552:563	Future exploitation	545:563	Future exploitation of this data	545:576	Future exploitation of this data is possible in a range of analyses and should yield information concerning genetic diversity, underlying genetic factors and the biological function of mucilage as an adaptive trait.
31281620	2	26	theme	outer	219:223	arg1	layer					225:229	The outer layer	215:229	The outer layer	215:229	The outer layer can be extracted with water facilitating the analysis of its major constituent, polysaccharides.
31281620	1	27	theme	Arabidopsis	129:139	arg1	seeds					142:146	Arabidopsis thaliana (Arabidopsis) seeds	107:146	Arabidopsis thaliana (Arabidopsis) seeds	107:146	Arabidopsis thaliana (Arabidopsis) seeds are myxospermous and release two layers of mucilage on imbibition.
31281620	4	28	theme	mucilage	730:737	arg1	diversity					661:669	genetic diversity	653:669	genetic diversity	653:669	Future exploitation of this data is possible in a range of analyses and should yield information concerning genetic diversity, underlying genetic factors and the biological function of mucilage as an adaptive trait.
31281620	4	28	theme	mucilage	730:737	arg1	factors					691:697	underlying genetic factors	672:697	underlying genetic factors	672:697	Future exploitation of this data is possible in a range of analyses and should yield information concerning genetic diversity, underlying genetic factors and the biological function of mucilage as an adaptive trait.
31281620	4	28	theme	mucilage	730:737	arg1	function					718:725	the biological function	703:725	the biological function of mucilage	703:737	Future exploitation of this data is possible in a range of analyses and should yield information concerning genetic diversity, underlying genetic factors and the biological function of mucilage as an adaptive trait.
31281620	1	29	theme	Arabidopsis	107:117	arg1	seeds					142:146	Arabidopsis thaliana (Arabidopsis) seeds	107:146	Arabidopsis thaliana (Arabidopsis) seeds	107:146	Arabidopsis thaliana (Arabidopsis) seeds are myxospermous and release two layers of mucilage on imbibition.
31281620	4	30	theme	adaptive	745:752	arg1	information					630:640	information	630:640	information concerning genetic diversity, underlying genetic factors and the biological function of mucilage	630:737	Future exploitation of this data is possible in a range of analyses and should yield information concerning genetic diversity, underlying genetic factors and the biological function of mucilage as an adaptive trait.
31281620	4	30	theme	adaptive	745:752	arg1	trait					754:758	an adaptive trait	742:758	an adaptive trait	742:758	Future exploitation of this data is possible in a range of analyses and should yield information concerning genetic diversity, underlying genetic factors and the biological function of mucilage as an adaptive trait.
31281620	0	31	from	properties	32:41	arg1	seeds					66:70	seeds	66:70	seeds of Arabidopsis natural accessions	66:104	Composition and physicochemical properties of outer mucilage from seeds of Arabidopsis natural accessions.
31281620	3	32	dep	biological	513:522	arg1	replicates					524:533	replicates	524:533	replicates	524:533	The composition and properties of outer mucilage have been determined for 306 natural accessions and six control genotypes to generate a data set comprising six traits measured in four biological replicates for each.
31281620	4	33	theme	genetic	683:689	arg1	factors					691:697	underlying genetic factors	672:697	underlying genetic factors	672:697	Future exploitation of this data is possible in a range of analyses and should yield information concerning genetic diversity, underlying genetic factors and the biological function of mucilage as an adaptive trait.
31281620	0	34	theme	accessions	95:104	arg1	seeds					66:70	seeds	66:70	seeds of Arabidopsis natural accessions	66:104	Composition and physicochemical properties of outer mucilage from seeds of Arabidopsis natural accessions.
29654535	0	0	theme	Pre-loaded	94:103	arg1	Picodiscs					126:134	Pre-loaded and Passively Loaded Picodiscs	94:134	Pre-loaded and Passively Loaded Picodiscs	94:134	Detecting Protein-Glycolipid Interactions Using CaR-ESI-MS and Model Membranes: Comparison of Pre-loaded and Passively Loaded Picodiscs.
29654535	3	1	theme	cholera	685:691	arg1	CTB5					723:726	CTB5	723:726	CTB5	723:726	The present work compares the use of conventional (pre-loaded) PDs with passively loaded PDs (PLPDs) for CaR-ESI-MS screening of glycolipids against cholera toxin B subunit homopentamer (CTB5).
29654535	3	1	theme	cholera	685:691	arg1	homopentamer					709:720	cholera toxin B subunit homopentamer	685:720	cholera toxin B subunit homopentamer (CTB5)	685:727	The present work compares the use of conventional (pre-loaded) PDs with passively loaded PDs (PLPDs) for CaR-ESI-MS screening of glycolipids against cholera toxin B subunit homopentamer (CTB5).
29654535	4	2	theme	lipids	834:839	arg1	mixture					823:829	a mixture	821:829	a mixture of lipids extracted from tissue	821:861	The pre-loaded PDs were prepared from a mixture of purified glycolipid and phospholipid or a mixture of lipids extracted from tissue, while the PLPDs were prepared by incubating PDs containing only phospholipid with glycolipid-containing lipid mixtures in aqueous solution.
29654535	4	2	theme	lipids	834:839	arg1	mixture					770:776	a mixture	768:776	a mixture of purified glycolipid and phospholipid	768:816	The pre-loaded PDs were prepared from a mixture of purified glycolipid and phospholipid or a mixture of lipids extracted from tissue, while the PLPDs were prepared by incubating PDs containing only phospholipid with glycolipid-containing lipid mixtures in aqueous solution.
29654535	7	3	theme	pre-loaded	1440:1449	arg1	PDs					1451:1453	pre-loaded PDs	1440:1453	pre-loaded PDs produced directly from GM1 at the same concentration	1440:1506	At incubation times ≥ 10 days, GM1 binding to CTB5 was indistinguishable from that observed with pre-loaded PDs produced directly from GM1 at the same concentration.
29654535	1	4	theme	promising	257:265	arg1	approach					267:274	a promising approach	255:274	a promising approach for the discovery of glycolipid ligands of glycan-binding proteins (GBPs)	255:348	Catch-and-release electrospray ionization mass spectrometry (CaR-ESI-MS), implemented using model membranes (MMs), is a promising approach for the discovery of glycolipid ligands of glycan-binding proteins (GBPs).
29654535	1	4	theme	promising	257:265	arg1	spectrometry					184:195	Catch-and-release electrospray ionization mass spectrometry	137:195	Catch-and-release electrospray ionization mass spectrometry (CaR-ESI-MS)	137:208	Catch-and-release electrospray ionization mass spectrometry (CaR-ESI-MS), implemented using model membranes (MMs), is a promising approach for the discovery of glycolipid ligands of glycan-binding proteins (GBPs).
29654535	4	5	theme	glycolipid-containing	946:966	arg1	mixtures					974:981	glycolipid-containing lipid mixtures	946:981	glycolipid-containing lipid mixtures in aqueous solution	946:1001	The pre-loaded PDs were prepared from a mixture of purified glycolipid and phospholipid or a mixture of lipids extracted from tissue, while the PLPDs were prepared by incubating PDs containing only phospholipid with glycolipid-containing lipid mixtures in aqueous solution.
29654535	9	6	dep	suggest	1778:1784	arg1	have					1796:1799	have	1796:1799	suggest PLPDs may have advantages over conventionally prepared PDs for screening glycolipids against GBPs using CaR-ESI-MS	1778:1899	Together, the results of this study suggest PLPDs may have advantages over conventionally prepared PDs for screening glycolipids against GBPs using CaR-ESI-MS.
29654535	2	7	theme	such	522:525	arg1	studies					527:533	such studies	522:533	such studies	522:533	Picodiscs (PDs), which are lipid-transporting complexes composed of the human sphingolipid activator protein saposin A and phospholipids, have proven to be useful MMs for such studies.
29654535	3	8	theme	glycolipids	665:675	arg1	screening					652:660	CaR-ESI-MS screening	641:660	CaR-ESI-MS screening of glycolipids against cholera toxin B subunit homopentamer (CTB5)	641:727	The present work compares the use of conventional (pre-loaded) PDs with passively loaded PDs (PLPDs) for CaR-ESI-MS screening of glycolipids against cholera toxin B subunit homopentamer (CTB5).
29654535	6	9	theme	GM1	1270:1272	arg1	incorporation					1274:1286	GM1 incorporation	1270:1286	GM1 incorporation into PDs	1270:1295	GM1 incorporation into PDs was evident within a few hours of incubation.
29654535	4	10	theme	pre-loaded	734:743	arg1	PDs					745:747	The pre-loaded PDs	730:747	The pre-loaded PDs	730:747	The pre-loaded PDs were prepared from a mixture of purified glycolipid and phospholipid or a mixture of lipids extracted from tissue, while the PLPDs were prepared by incubating PDs containing only phospholipid with glycolipid-containing lipid mixtures in aqueous solution.
29654535	7	11	from	that	1421:1424	arg1	indistinguishable					1398:1414	indistinguishable	1398:1414	indistinguishable	1398:1414	At incubation times ≥ 10 days, GM1 binding to CTB5 was indistinguishable from that observed with pre-loaded PDs produced directly from GM1 at the same concentration.
29654535	8	12	theme	greater	1703:1709	arg1	number					1711:1716	a greater number	1701:1716	a greater number of ganglioside ligands	1701:1739	Comparison of ganglioside binding to CTB5 measured for pre-loaded PDs and PLPDs prepared from glycolipids extracted from pig and mouse brain revealed that the PLPDs allow for the detection of a greater number of ganglioside ligands.
29654535	3	13	theme	subunit	701:707	arg1	CTB5					723:726	CTB5	723:726	CTB5	723:726	The present work compares the use of conventional (pre-loaded) PDs with passively loaded PDs (PLPDs) for CaR-ESI-MS screening of glycolipids against cholera toxin B subunit homopentamer (CTB5).
29654535	3	13	theme	subunit	701:707	arg1	homopentamer					709:720	cholera toxin B subunit homopentamer	685:720	cholera toxin B subunit homopentamer (CTB5)	685:727	The present work compares the use of conventional (pre-loaded) PDs with passively loaded PDs (PLPDs) for CaR-ESI-MS screening of glycolipids against cholera toxin B subunit homopentamer (CTB5).
29654535	8	14	theme	ligands	1733:1739	arg1	number					1711:1716	a greater number	1701:1716	a greater number of ganglioside ligands	1701:1739	Comparison of ganglioside binding to CTB5 measured for pre-loaded PDs and PLPDs prepared from glycolipids extracted from pig and mouse brain revealed that the PLPDs allow for the detection of a greater number of ganglioside ligands.
29654535	3	15	theme	present	540:546	arg1	work					548:551	The present work	536:551	The present work	536:551	The present work compares the use of conventional (pre-loaded) PDs with passively loaded PDs (PLPDs) for CaR-ESI-MS screening of glycolipids against cholera toxin B subunit homopentamer (CTB5).
29654535	0	16	theme	Picodiscs	126:134	arg1	Comparison					80:89	Comparison	80:89	Comparison of Pre-loaded and Passively Loaded Picodiscs	80:134	Detecting Protein-Glycolipid Interactions Using CaR-ESI-MS and Model Membranes: Comparison of Pre-loaded and Passively Loaded Picodiscs.
29654535	8	17	theme	number	1711:1716	arg1	detection					1688:1696	the detection	1684:1696	the detection of a greater number of ganglioside ligands	1684:1739	Comparison of ganglioside binding to CTB5 measured for pre-loaded PDs and PLPDs prepared from glycolipids extracted from pig and mouse brain revealed that the PLPDs allow for the detection of a greater number of ganglioside ligands.
29654535	0	18	theme	Loaded	119:124	arg1	Picodiscs					126:134	Pre-loaded and Passively Loaded Picodiscs	94:134	Pre-loaded and Passively Loaded Picodiscs	94:134	Detecting Protein-Glycolipid Interactions Using CaR-ESI-MS and Model Membranes: Comparison of Pre-loaded and Passively Loaded Picodiscs.
29654535	3	19	theme	toxin	693:697	arg1	CTB5					723:726	CTB5	723:726	CTB5	723:726	The present work compares the use of conventional (pre-loaded) PDs with passively loaded PDs (PLPDs) for CaR-ESI-MS screening of glycolipids against cholera toxin B subunit homopentamer (CTB5).
29654535	3	19	theme	toxin	693:697	arg1	homopentamer					709:720	cholera toxin B subunit homopentamer	685:720	cholera toxin B subunit homopentamer (CTB5)	685:727	The present work compares the use of conventional (pre-loaded) PDs with passively loaded PDs (PLPDs) for CaR-ESI-MS screening of glycolipids against cholera toxin B subunit homopentamer (CTB5).
29654535	4	20	theme	phospholipid	805:816	arg1	mixture					823:829	a mixture	821:829	a mixture of lipids extracted from tissue	821:861	The pre-loaded PDs were prepared from a mixture of purified glycolipid and phospholipid or a mixture of lipids extracted from tissue, while the PLPDs were prepared by incubating PDs containing only phospholipid with glycolipid-containing lipid mixtures in aqueous solution.
29654535	4	20	theme	phospholipid	805:816	arg1	mixture					770:776	a mixture	768:776	a mixture of purified glycolipid and phospholipid	768:816	The pre-loaded PDs were prepared from a mixture of purified glycolipid and phospholipid or a mixture of lipids extracted from tissue, while the PLPDs were prepared by incubating PDs containing only phospholipid with glycolipid-containing lipid mixtures in aqueous solution.
29654535	8	21	theme	pre-loaded	1564:1573	arg1	PDs					1575:1577	pre-loaded PDs	1564:1577	pre-loaded PDs	1564:1577	Comparison of ganglioside binding to CTB5 measured for pre-loaded PDs and PLPDs prepared from glycolipids extracted from pig and mouse brain revealed that the PLPDs allow for the detection of a greater number of ganglioside ligands.
29654535	2	22	theme	lipid-transporting	378:395	arg1	complexes					397:405	lipid-transporting complexes	378:405	lipid-transporting complexes composed of the human sphingolipid activator protein saposin A and phospholipids	378:486	Picodiscs (PDs), which are lipid-transporting complexes composed of the human sphingolipid activator protein saposin A and phospholipids, have proven to be useful MMs for such studies.
29654535	2	22	theme	lipid-transporting	378:395	arg1	Picodiscs					351:359	Picodiscs	351:359	Picodiscs (PDs)	351:365	Picodiscs (PDs), which are lipid-transporting complexes composed of the human sphingolipid activator protein saposin A and phospholipids, have proven to be useful MMs for such studies.
29654535	0	23	theme	Protein-Glycolipid	10:27	arg1	Interactions					29:40	Protein-Glycolipid Interactions	10:40	Protein-Glycolipid Interactions Using CaR-ESI-MS and Model Membranes	10:77	Detecting Protein-Glycolipid Interactions Using CaR-ESI-MS and Model Membranes: Comparison of Pre-loaded and Passively Loaded Picodiscs.
29654535	3	24	theme	B	699:699	arg1	CTB5					723:726	CTB5	723:726	CTB5	723:726	The present work compares the use of conventional (pre-loaded) PDs with passively loaded PDs (PLPDs) for CaR-ESI-MS screening of glycolipids against cholera toxin B subunit homopentamer (CTB5).
29654535	3	24	theme	B	699:699	arg1	homopentamer					709:720	cholera toxin B subunit homopentamer	685:720	cholera toxin B subunit homopentamer (CTB5)	685:727	The present work compares the use of conventional (pre-loaded) PDs with passively loaded PDs (PLPDs) for CaR-ESI-MS screening of glycolipids against cholera toxin B subunit homopentamer (CTB5).
29654535	5	25	theme	PD	1191:1192	arg1	ions					1194:1197	the gaseous PD ions	1179:1197	the gaseous PD ions	1179:1197	Time-dependent changes in the composition of the PLPDs produced by incubation with glycomicelles of the ganglioside GM1 were monitored using collision-induced dissociation of the gaseous PD ions and from the extent of ganglioside binding to CTB5 measured by ESI-MS.
29654535	4	26	from	mixtures	974:981	arg1	solution					994:1001	aqueous solution	986:1001	aqueous solution	986:1001	The pre-loaded PDs were prepared from a mixture of purified glycolipid and phospholipid or a mixture of lipids extracted from tissue, while the PLPDs were prepared by incubating PDs containing only phospholipid with glycolipid-containing lipid mixtures in aqueous solution.
29654535	8	27	theme	ganglioside	1721:1731	arg1	ligands					1733:1739	ganglioside ligands	1721:1739	ganglioside ligands	1721:1739	Comparison of ganglioside binding to CTB5 measured for pre-loaded PDs and PLPDs prepared from glycolipids extracted from pig and mouse brain revealed that the PLPDs allow for the detection of a greater number of ganglioside ligands.
29654535	2	28	theme	protein	452:458	arg1	A					468:468	the human sphingolipid activator protein saposin A	419:468	the human sphingolipid activator protein saposin A	419:468	Picodiscs (PDs), which are lipid-transporting complexes composed of the human sphingolipid activator protein saposin A and phospholipids, have proven to be useful MMs for such studies.
29654535	7	29	theme	same	1489:1492	arg1	concentration					1494:1506	the same concentration	1485:1506	the same concentration	1485:1506	At incubation times ≥ 10 days, GM1 binding to CTB5 was indistinguishable from that observed with pre-loaded PDs produced directly from GM1 at the same concentration.
29654535	5	30	from	changes	1019:1025	arg1	composition					1034:1044	the composition	1030:1044	the composition of the PLPDs produced by incubation with glycomicelles of the ganglioside GM1	1030:1122	Time-dependent changes in the composition of the PLPDs produced by incubation with glycomicelles of the ganglioside GM1 were monitored using collision-induced dissociation of the gaseous PD ions and from the extent of ganglioside binding to CTB5 measured by ESI-MS.
29654535	2	31	theme	activator	442:450	arg1	A					468:468	the human sphingolipid activator protein saposin A	419:468	the human sphingolipid activator protein saposin A	419:468	Picodiscs (PDs), which are lipid-transporting complexes composed of the human sphingolipid activator protein saposin A and phospholipids, have proven to be useful MMs for such studies.
29654535	5	32	theme	gaseous	1183:1189	arg1	ions					1194:1197	the gaseous PD ions	1179:1197	the gaseous PD ions	1179:1197	Time-dependent changes in the composition of the PLPDs produced by incubation with glycomicelles of the ganglioside GM1 were monitored using collision-induced dissociation of the gaseous PD ions and from the extent of ganglioside binding to CTB5 measured by ESI-MS.
29654535	6	33	theme	incubation	1331:1340	arg1	hours					1322:1326	a few hours	1316:1326	a few hours of incubation	1316:1340	GM1 incorporation into PDs was evident within a few hours of incubation.
29654535	4	34	theme	glycolipid	790:799	arg1	mixture					823:829	a mixture	821:829	a mixture of lipids extracted from tissue	821:861	The pre-loaded PDs were prepared from a mixture of purified glycolipid and phospholipid or a mixture of lipids extracted from tissue, while the PLPDs were prepared by incubating PDs containing only phospholipid with glycolipid-containing lipid mixtures in aqueous solution.
29654535	4	34	theme	glycolipid	790:799	arg1	mixture					770:776	a mixture	768:776	a mixture of purified glycolipid and phospholipid	768:816	The pre-loaded PDs were prepared from a mixture of purified glycolipid and phospholipid or a mixture of lipids extracted from tissue, while the PLPDs were prepared by incubating PDs containing only phospholipid with glycolipid-containing lipid mixtures in aqueous solution.
29654535	5	35	with	incubation	1071:1080	arg1	glycomicelles					1087:1099	glycomicelles	1087:1099	glycomicelles of the ganglioside GM1	1087:1122	Time-dependent changes in the composition of the PLPDs produced by incubation with glycomicelles of the ganglioside GM1 were monitored using collision-induced dissociation of the gaseous PD ions and from the extent of ganglioside binding to CTB5 measured by ESI-MS.
29654535	1	36	theme	model	229:233	arg1	membranes					235:243	model membranes	229:243	model membranes (MMs)	229:249	Catch-and-release electrospray ionization mass spectrometry (CaR-ESI-MS), implemented using model membranes (MMs), is a promising approach for the discovery of glycolipid ligands of glycan-binding proteins (GBPs).
29654535	1	36	theme	model	229:233	arg1	MMs					246:248	MMs	246:248	MMs	246:248	Catch-and-release electrospray ionization mass spectrometry (CaR-ESI-MS), implemented using model membranes (MMs), is a promising approach for the discovery of glycolipid ligands of glycan-binding proteins (GBPs).
29654535	2	37	theme	sphingolipid	429:440	arg1	A					468:468	the human sphingolipid activator protein saposin A	419:468	the human sphingolipid activator protein saposin A	419:468	Picodiscs (PDs), which are lipid-transporting complexes composed of the human sphingolipid activator protein saposin A and phospholipids, have proven to be useful MMs for such studies.
29654535	1	38	theme	glycolipid	297:306	arg1	ligands					308:314	glycolipid ligands	297:314	glycolipid ligands	297:314	Catch-and-release electrospray ionization mass spectrometry (CaR-ESI-MS), implemented using model membranes (MMs), is a promising approach for the discovery of glycolipid ligands of glycan-binding proteins (GBPs).
29654535	5	39	from	extent	1212:1217	arg1	dissociation					1163:1174	collision-induced dissociation	1145:1174	collision-induced dissociation of the gaseous PD ions and from the extent of ganglioside binding to CTB5 measured by ESI-MS	1145:1267	Time-dependent changes in the composition of the PLPDs produced by incubation with glycomicelles of the ganglioside GM1 were monitored using collision-induced dissociation of the gaseous PD ions and from the extent of ganglioside binding to CTB5 measured by ESI-MS.
29654535	5	40	theme	PLPDs	1053:1057	arg1	composition					1034:1044	the composition	1030:1044	the composition of the PLPDs produced by incubation with glycomicelles of the ganglioside GM1	1030:1122	Time-dependent changes in the composition of the PLPDs produced by incubation with glycomicelles of the ganglioside GM1 were monitored using collision-induced dissociation of the gaseous PD ions and from the extent of ganglioside binding to CTB5 measured by ESI-MS.
29654535	7	41	from	10 days	1365:1371	arg1	indistinguishable					1398:1414	indistinguishable	1398:1414	indistinguishable	1398:1414	At incubation times ≥ 10 days, GM1 binding to CTB5 was indistinguishable from that observed with pre-loaded PDs produced directly from GM1 at the same concentration.
29654535	5	42	theme	GM1	1120:1122	arg1	glycomicelles					1087:1099	glycomicelles	1087:1099	glycomicelles of the ganglioside GM1	1087:1122	Time-dependent changes in the composition of the PLPDs produced by incubation with glycomicelles of the ganglioside GM1 were monitored using collision-induced dissociation of the gaseous PD ions and from the extent of ganglioside binding to CTB5 measured by ESI-MS.
29654535	5	43	theme	ions	1194:1197	arg1	dissociation					1163:1174	collision-induced dissociation	1145:1174	collision-induced dissociation of the gaseous PD ions and from the extent of ganglioside binding to CTB5 measured by ESI-MS	1145:1267	Time-dependent changes in the composition of the PLPDs produced by incubation with glycomicelles of the ganglioside GM1 were monitored using collision-induced dissociation of the gaseous PD ions and from the extent of ganglioside binding to CTB5 measured by ESI-MS.
29654535	1	44	theme	ligands	308:314	arg1	discovery					284:292	the discovery	280:292	the discovery of glycolipid ligands of glycan-binding proteins (GBPs)	280:348	Catch-and-release electrospray ionization mass spectrometry (CaR-ESI-MS), implemented using model membranes (MMs), is a promising approach for the discovery of glycolipid ligands of glycan-binding proteins (GBPs).
29654535	6	45	theme	few	1318:1320	arg1	hours					1322:1326	a few hours	1316:1326	a few hours of incubation	1316:1340	GM1 incorporation into PDs was evident within a few hours of incubation.
29654535	4	46	theme	purified	781:788	arg1	glycolipid					790:799	glycolipid	790:799	glycolipid	790:799	The pre-loaded PDs were prepared from a mixture of purified glycolipid and phospholipid or a mixture of lipids extracted from tissue, while the PLPDs were prepared by incubating PDs containing only phospholipid with glycolipid-containing lipid mixtures in aqueous solution.
29654535	0	47	theme	CaR-ESI-MS	48:57	arg1	Membranes					69:77	CaR-ESI-MS and Model Membranes	48:77	Membranes	69:77	Detecting Protein-Glycolipid Interactions Using CaR-ESI-MS and Model Membranes: Comparison of Pre-loaded and Passively Loaded Picodiscs.
29654535	4	48	theme	aqueous	986:992	arg1	solution					994:1001	aqueous solution	986:1001	aqueous solution	986:1001	The pre-loaded PDs were prepared from a mixture of purified glycolipid and phospholipid or a mixture of lipids extracted from tissue, while the PLPDs were prepared by incubating PDs containing only phospholipid with glycolipid-containing lipid mixtures in aqueous solution.
29654535	3	49	theme	loaded	618:623	arg1	PLPDs					630:634	PLPDs	630:634	PLPDs	630:634	The present work compares the use of conventional (pre-loaded) PDs with passively loaded PDs (PLPDs) for CaR-ESI-MS screening of glycolipids against cholera toxin B subunit homopentamer (CTB5).
29654535	3	49	theme	loaded	618:623	arg1	PDs					625:627	passively loaded PDs	608:627	passively loaded PDs (PLPDs)	608:635	The present work compares the use of conventional (pre-loaded) PDs with passively loaded PDs (PLPDs) for CaR-ESI-MS screening of glycolipids against cholera toxin B subunit homopentamer (CTB5).
29654535	3	50	theme	pre-loaded	587:596	arg1	PDs					599:601	conventional (pre-loaded) PDs	573:601	conventional (pre-loaded) PDs with passively loaded PDs (PLPDs)	573:635	The present work compares the use of conventional (pre-loaded) PDs with passively loaded PDs (PLPDs) for CaR-ESI-MS screening of glycolipids against cholera toxin B subunit homopentamer (CTB5).
29654535	4	51	contain	containing	912:921	arg1	PDs					908:910	PDs	908:910	PDs containing only phospholipid	908:939	The pre-loaded PDs were prepared from a mixture of purified glycolipid and phospholipid or a mixture of lipids extracted from tissue, while the PLPDs were prepared by incubating PDs containing only phospholipid with glycolipid-containing lipid mixtures in aqueous solution.
29654535	4	51	contain	containing	912:921	arg2	phospholipid					928:939	only phospholipid	923:939	only phospholipid	923:939	The pre-loaded PDs were prepared from a mixture of purified glycolipid and phospholipid or a mixture of lipids extracted from tissue, while the PLPDs were prepared by incubating PDs containing only phospholipid with glycolipid-containing lipid mixtures in aqueous solution.
29654535	2	52	theme	useful	507:512	arg1	MMs					514:516	useful MMs	507:516	useful MMs for such studies	507:533	Picodiscs (PDs), which are lipid-transporting complexes composed of the human sphingolipid activator protein saposin A and phospholipids, have proven to be useful MMs for such studies.
29654535	1	53	theme	glycan-binding	319:332	arg1	GBPs					344:347	GBPs	344:347	GBPs	344:347	Catch-and-release electrospray ionization mass spectrometry (CaR-ESI-MS), implemented using model membranes (MMs), is a promising approach for the discovery of glycolipid ligands of glycan-binding proteins (GBPs).
29654535	1	53	theme	glycan-binding	319:332	arg1	proteins					334:341	glycan-binding proteins	319:341	glycan-binding proteins (GBPs)	319:348	Catch-and-release electrospray ionization mass spectrometry (CaR-ESI-MS), implemented using model membranes (MMs), is a promising approach for the discovery of glycolipid ligands of glycan-binding proteins (GBPs).
29654535	10	54	theme	Graphical	1902:1910	arg1	Abstract					1912:1919	Graphical Abstract	1902:1919	Graphical Abstract	1902:1919	Graphical Abstract ᅟ.
29654535	8	55	theme	binding	1535:1541	arg1	Comparison					1509:1518	Comparison	1509:1518	Comparison of ganglioside binding to CTB5 measured for pre-loaded PDs and PLPDs prepared from glycolipids extracted from pig and mouse brain	1509:1648	Comparison of ganglioside binding to CTB5 measured for pre-loaded PDs and PLPDs prepared from glycolipids extracted from pig and mouse brain revealed that the PLPDs allow for the detection of a greater number of ganglioside ligands.
29654535	8	56	theme	ganglioside	1523:1533	arg1	binding					1535:1541	ganglioside binding	1523:1541	ganglioside binding	1523:1541	Comparison of ganglioside binding to CTB5 measured for pre-loaded PDs and PLPDs prepared from glycolipids extracted from pig and mouse brain revealed that the PLPDs allow for the detection of a greater number of ganglioside ligands.
29654535	0	57	theme	Model	63:67	arg1	Membranes					69:77	CaR-ESI-MS and Model Membranes	48:77	Membranes	69:77	Detecting Protein-Glycolipid Interactions Using CaR-ESI-MS and Model Membranes: Comparison of Pre-loaded and Passively Loaded Picodiscs.
29654535	1	58	theme	proteins	334:341	arg1	discovery					284:292	the discovery	280:292	the discovery of glycolipid ligands of glycan-binding proteins (GBPs)	280:348	Catch-and-release electrospray ionization mass spectrometry (CaR-ESI-MS), implemented using model membranes (MMs), is a promising approach for the discovery of glycolipid ligands of glycan-binding proteins (GBPs).
29654535	5	59	theme	ganglioside	1108:1118	arg1	GM1					1120:1122	the ganglioside GM1	1104:1122	the ganglioside GM1	1104:1122	Time-dependent changes in the composition of the PLPDs produced by incubation with glycomicelles of the ganglioside GM1 were monitored using collision-induced dissociation of the gaseous PD ions and from the extent of ganglioside binding to CTB5 measured by ESI-MS.
29654535	3	60	theme	CaR-ESI-MS	641:650	arg1	screening					652:660	CaR-ESI-MS screening	641:660	CaR-ESI-MS screening of glycolipids against cholera toxin B subunit homopentamer (CTB5)	641:727	The present work compares the use of conventional (pre-loaded) PDs with passively loaded PDs (PLPDs) for CaR-ESI-MS screening of glycolipids against cholera toxin B subunit homopentamer (CTB5).
29654535	3	61	theme	conventional	573:584	arg1	PDs					599:601	conventional (pre-loaded) PDs	573:601	conventional (pre-loaded) PDs with passively loaded PDs (PLPDs)	573:635	The present work compares the use of conventional (pre-loaded) PDs with passively loaded PDs (PLPDs) for CaR-ESI-MS screening of glycolipids against cholera toxin B subunit homopentamer (CTB5).
29654535	7	62	from	concentration	1494:1506	arg1	GM1					1478:1480	GM1	1478:1480	GM1 at the same concentration	1478:1506	At incubation times ≥ 10 days, GM1 binding to CTB5 was indistinguishable from that observed with pre-loaded PDs produced directly from GM1 at the same concentration.
29654535	1	63	theme	Catch-and-release	137:153	arg1	spectrometry					184:195	Catch-and-release electrospray ionization mass spectrometry	137:195	Catch-and-release electrospray ionization mass spectrometry (CaR-ESI-MS)	137:208	Catch-and-release electrospray ionization mass spectrometry (CaR-ESI-MS), implemented using model membranes (MMs), is a promising approach for the discovery of glycolipid ligands of glycan-binding proteins (GBPs).
29654535	1	63	theme	Catch-and-release	137:153	arg1	CaR-ESI-MS					198:207	CaR-ESI-MS	198:207	CaR-ESI-MS	198:207	Catch-and-release electrospray ionization mass spectrometry (CaR-ESI-MS), implemented using model membranes (MMs), is a promising approach for the discovery of glycolipid ligands of glycan-binding proteins (GBPs).
29654535	1	63	theme	Catch-and-release	137:153	arg1	approach					267:274	a promising approach	255:274	a promising approach for the discovery of glycolipid ligands of glycan-binding proteins (GBPs)	255:348	Catch-and-release electrospray ionization mass spectrometry (CaR-ESI-MS), implemented using model membranes (MMs), is a promising approach for the discovery of glycolipid ligands of glycan-binding proteins (GBPs).
29654535	4	64	theme	only	923:926	arg1	phospholipid					928:939	only phospholipid	923:939	only phospholipid	923:939	The pre-loaded PDs were prepared from a mixture of purified glycolipid and phospholipid or a mixture of lipids extracted from tissue, while the PLPDs were prepared by incubating PDs containing only phospholipid with glycolipid-containing lipid mixtures in aqueous solution.
29654535	3	65	theme	PDs	599:601	arg1	use					566:568	the use	562:568	the use of conventional (pre-loaded) PDs with passively loaded PDs (PLPDs) for CaR-ESI-MS screening of glycolipids against cholera toxin B subunit homopentamer (CTB5)	562:727	The present work compares the use of conventional (pre-loaded) PDs with passively loaded PDs (PLPDs) for CaR-ESI-MS screening of glycolipids against cholera toxin B subunit homopentamer (CTB5).
29654535	5	66	theme	Time-dependent	1004:1017	arg1	changes					1019:1025	Time-dependent changes	1004:1025	Time-dependent changes in the composition of the PLPDs produced by incubation with glycomicelles of the ganglioside GM1	1004:1122	Time-dependent changes in the composition of the PLPDs produced by incubation with glycomicelles of the ganglioside GM1 were monitored using collision-induced dissociation of the gaseous PD ions and from the extent of ganglioside binding to CTB5 measured by ESI-MS.
29654535	1	67	theme	electrospray	155:166	arg1	spectrometry					184:195	Catch-and-release electrospray ionization mass spectrometry	137:195	Catch-and-release electrospray ionization mass spectrometry (CaR-ESI-MS)	137:208	Catch-and-release electrospray ionization mass spectrometry (CaR-ESI-MS), implemented using model membranes (MMs), is a promising approach for the discovery of glycolipid ligands of glycan-binding proteins (GBPs).
29654535	1	67	theme	electrospray	155:166	arg1	CaR-ESI-MS					198:207	CaR-ESI-MS	198:207	CaR-ESI-MS	198:207	Catch-and-release electrospray ionization mass spectrometry (CaR-ESI-MS), implemented using model membranes (MMs), is a promising approach for the discovery of glycolipid ligands of glycan-binding proteins (GBPs).
29654535	1	67	theme	electrospray	155:166	arg1	approach					267:274	a promising approach	255:274	a promising approach for the discovery of glycolipid ligands of glycan-binding proteins (GBPs)	255:348	Catch-and-release electrospray ionization mass spectrometry (CaR-ESI-MS), implemented using model membranes (MMs), is a promising approach for the discovery of glycolipid ligands of glycan-binding proteins (GBPs).
29654535	8	68	theme	mouse	1638:1642	arg1	brain					1644:1648	mouse brain	1638:1648	mouse brain	1638:1648	Comparison of ganglioside binding to CTB5 measured for pre-loaded PDs and PLPDs prepared from glycolipids extracted from pig and mouse brain revealed that the PLPDs allow for the detection of a greater number of ganglioside ligands.
29654535	7	69	from	times	1357:1361	arg1	indistinguishable					1398:1414	indistinguishable	1398:1414	indistinguishable	1398:1414	At incubation times ≥ 10 days, GM1 binding to CTB5 was indistinguishable from that observed with pre-loaded PDs produced directly from GM1 at the same concentration.
29654535	7	70	theme	incubation	1346:1355	arg1	times					1357:1361	incubation times	1346:1361	incubation times	1346:1361	At incubation times ≥ 10 days, GM1 binding to CTB5 was indistinguishable from that observed with pre-loaded PDs produced directly from GM1 at the same concentration.
29654535	1	71	theme	ionization	168:177	arg1	spectrometry					184:195	Catch-and-release electrospray ionization mass spectrometry	137:195	Catch-and-release electrospray ionization mass spectrometry (CaR-ESI-MS)	137:208	Catch-and-release electrospray ionization mass spectrometry (CaR-ESI-MS), implemented using model membranes (MMs), is a promising approach for the discovery of glycolipid ligands of glycan-binding proteins (GBPs).
29654535	1	71	theme	ionization	168:177	arg1	CaR-ESI-MS					198:207	CaR-ESI-MS	198:207	CaR-ESI-MS	198:207	Catch-and-release electrospray ionization mass spectrometry (CaR-ESI-MS), implemented using model membranes (MMs), is a promising approach for the discovery of glycolipid ligands of glycan-binding proteins (GBPs).
29654535	1	71	theme	ionization	168:177	arg1	approach					267:274	a promising approach	255:274	a promising approach for the discovery of glycolipid ligands of glycan-binding proteins (GBPs)	255:348	Catch-and-release electrospray ionization mass spectrometry (CaR-ESI-MS), implemented using model membranes (MMs), is a promising approach for the discovery of glycolipid ligands of glycan-binding proteins (GBPs).
29654535	9	72	theme	study	1772:1776	arg1	results					1756:1762	the results	1752:1762	the results of this study	1752:1776	Together, the results of this study suggest PLPDs may have advantages over conventionally prepared PDs for screening glycolipids against GBPs using CaR-ESI-MS.
29654535	5	73	theme	ganglioside	1222:1232	arg1	binding					1234:1240	ganglioside binding	1222:1240	ganglioside binding to CTB5 measured by ESI-MS	1222:1267	Time-dependent changes in the composition of the PLPDs produced by incubation with glycomicelles of the ganglioside GM1 were monitored using collision-induced dissociation of the gaseous PD ions and from the extent of ganglioside binding to CTB5 measured by ESI-MS.
29654535	2	74	theme	human	423:427	arg1	A					468:468	the human sphingolipid activator protein saposin A	419:468	the human sphingolipid activator protein saposin A	419:468	Picodiscs (PDs), which are lipid-transporting complexes composed of the human sphingolipid activator protein saposin A and phospholipids, have proven to be useful MMs for such studies.
29654535	1	75	theme	mass	179:182	arg1	spectrometry					184:195	Catch-and-release electrospray ionization mass spectrometry	137:195	Catch-and-release electrospray ionization mass spectrometry (CaR-ESI-MS)	137:208	Catch-and-release electrospray ionization mass spectrometry (CaR-ESI-MS), implemented using model membranes (MMs), is a promising approach for the discovery of glycolipid ligands of glycan-binding proteins (GBPs).
29654535	1	75	theme	mass	179:182	arg1	CaR-ESI-MS					198:207	CaR-ESI-MS	198:207	CaR-ESI-MS	198:207	Catch-and-release electrospray ionization mass spectrometry (CaR-ESI-MS), implemented using model membranes (MMs), is a promising approach for the discovery of glycolipid ligands of glycan-binding proteins (GBPs).
29654535	1	75	theme	mass	179:182	arg1	approach					267:274	a promising approach	255:274	a promising approach for the discovery of glycolipid ligands of glycan-binding proteins (GBPs)	255:348	Catch-and-release electrospray ionization mass spectrometry (CaR-ESI-MS), implemented using model membranes (MMs), is a promising approach for the discovery of glycolipid ligands of glycan-binding proteins (GBPs).
29654535	5	76	theme	binding	1234:1240	arg1	extent					1212:1217	the extent	1208:1217	the extent of ganglioside binding to CTB5 measured by ESI-MS	1208:1267	Time-dependent changes in the composition of the PLPDs produced by incubation with glycomicelles of the ganglioside GM1 were monitored using collision-induced dissociation of the gaseous PD ions and from the extent of ganglioside binding to CTB5 measured by ESI-MS.
29654535	3	77	with	PDs	599:601	arg1	PLPDs					630:634	PLPDs	630:634	PLPDs	630:634	The present work compares the use of conventional (pre-loaded) PDs with passively loaded PDs (PLPDs) for CaR-ESI-MS screening of glycolipids against cholera toxin B subunit homopentamer (CTB5).
29654535	3	77	with	PDs	599:601	arg1	PDs					625:627	passively loaded PDs	608:627	passively loaded PDs (PLPDs)	608:635	The present work compares the use of conventional (pre-loaded) PDs with passively loaded PDs (PLPDs) for CaR-ESI-MS screening of glycolipids against cholera toxin B subunit homopentamer (CTB5).
29654535	4	78	theme	lipid	968:972	arg1	mixtures					974:981	glycolipid-containing lipid mixtures	946:981	glycolipid-containing lipid mixtures in aqueous solution	946:1001	The pre-loaded PDs were prepared from a mixture of purified glycolipid and phospholipid or a mixture of lipids extracted from tissue, while the PLPDs were prepared by incubating PDs containing only phospholipid with glycolipid-containing lipid mixtures in aqueous solution.
29654535	0	79	dep	Detecting	0:8	arg1	Comparison					80:89	Comparison	80:89	Comparison of Pre-loaded and Passively Loaded Picodiscs	80:134	Detecting Protein-Glycolipid Interactions Using CaR-ESI-MS and Model Membranes: Comparison of Pre-loaded and Passively Loaded Picodiscs.
29654535	9	80	theme	prepared	1832:1839	arg1	PDs					1841:1843	conventionally prepared PDs	1817:1843	conventionally prepared PDs	1817:1843	Together, the results of this study suggest PLPDs may have advantages over conventionally prepared PDs for screening glycolipids against GBPs using CaR-ESI-MS.
29654535	2	81	theme	saposin	460:466	arg1	A					468:468	the human sphingolipid activator protein saposin A	419:468	the human sphingolipid activator protein saposin A	419:468	Picodiscs (PDs), which are lipid-transporting complexes composed of the human sphingolipid activator protein saposin A and phospholipids, have proven to be useful MMs for such studies.
29654535	5	82	theme	collision-induced	1145:1161	arg1	dissociation					1163:1174	collision-induced dissociation	1145:1174	collision-induced dissociation of the gaseous PD ions and from the extent of ganglioside binding to CTB5 measured by ESI-MS	1145:1267	Time-dependent changes in the composition of the PLPDs produced by incubation with glycomicelles of the ganglioside GM1 were monitored using collision-induced dissociation of the gaseous PD ions and from the extent of ganglioside binding to CTB5 measured by ESI-MS.
30716420	13	0	theme	gut	1898:1900	arg1	composition					1913:1923	shaping gut microbiota composition	1890:1923	shaping gut microbiota composition in relation to susceptibility to colitis	1890:1964	CONCLUSIONS These findings demonstrate a novel role of gut-derived 5-HT in shaping gut microbiota composition in relation to susceptibility to colitis, identifying 5-HT-microbiota axis as a potential new therapeutic target in intestinal inflammatory disorders.
30716420	6	1	theme	indirect	804:811	arg1	influence					813:821	The indirect influence	800:821	The indirect influence of 5-HT on microbiota	800:843	The indirect influence of 5-HT on microbiota was assessed by measuring antimicrobial peptides, specifically β-defensins, in the colon of mice and HT-29 colonic epithelial cells.
30716420	5	2	from	influence	708:716	arg1	bacteria					729:736	bacteria	729:736	bacteria	729:736	Direct influence of 5-HT on bacteria was assessed by using in vitro system of isolated commensals.
30716420	13	3	theme	gut-derived	1870:1880	arg1	5-HT					1882:1885	gut-derived 5-HT	1870:1885	gut-derived 5-HT	1870:1885	CONCLUSIONS These findings demonstrate a novel role of gut-derived 5-HT in shaping gut microbiota composition in relation to susceptibility to colitis, identifying 5-HT-microbiota axis as a potential new therapeutic target in intestinal inflammatory disorders.
30716420	7	4	theme	gut	1097:1099	arg1	microbiota					1101:1110	gut microbiota	1097:1110	gut microbiota	1097:1110	The impact of gut microbiota on the development of dextran sulfate sodium-induced colitis was assessed by transferring gut microbiota from Tph1-/- mice to Tph1+/- littermates and vice versa, as well as in germ-free mice.
30716420	13	5	theme	therapeutic	2019:2029	arg1	axis					1995:1998	5-HT-microbiota axis	1979:1998	5-HT-microbiota axis	1979:1998	CONCLUSIONS These findings demonstrate a novel role of gut-derived 5-HT in shaping gut microbiota composition in relation to susceptibility to colitis, identifying 5-HT-microbiota axis as a potential new therapeutic target in intestinal inflammatory disorders.
30716420	13	5	theme	therapeutic	2019:2029	arg1	target					2031:2036	a potential new therapeutic target	2003:2036	a potential new therapeutic target in intestinal inflammatory disorders	2003:2073	CONCLUSIONS These findings demonstrate a novel role of gut-derived 5-HT in shaping gut microbiota composition in relation to susceptibility to colitis, identifying 5-HT-microbiota axis as a potential new therapeutic target in intestinal inflammatory disorders.
30716420	12	6	theme	sodium-induced	1791:1804	arg1	colitis					1806:1812	dextran sulfate sodium-induced colitis	1775:1812	less severe dextran sulfate sodium-induced colitis	1763:1812	Furthermore, germ-free mice colonized with microbiota from Tph1-/- mice exhibited less severe dextran sulfate sodium-induced colitis.
30716420	8	7	theme	microbial	1235:1243	arg1	composition					1245:1255	microbial composition	1235:1255	microbial composition	1235:1255	RESULTS A significant difference in microbial composition between Tph1-/- and Tph1+/- littermates was observed.
30716420	11	8	theme	colitis	1595:1601	arg1	severity					1603:1610	colitis severity	1595:1610	colitis severity	1595:1610	Microbial transfer from Tph1-/- to Tph1+/- littermates and vice versa altered colitis severity, with microbiota from Tph1-/- mice mediating the protective effects.
30716420	6	9	theme	colonic	952:958	arg1	cells					971:975	HT-29 colonic epithelial cells	946:975	HT-29 colonic epithelial cells	946:975	The indirect influence of 5-HT on microbiota was assessed by measuring antimicrobial peptides, specifically β-defensins, in the colon of mice and HT-29 colonic epithelial cells.
30716420	13	10	theme	inflammatory	2052:2063	arg1	disorders					2065:2073	intestinal inflammatory disorders	2041:2073	intestinal inflammatory disorders	2041:2073	CONCLUSIONS These findings demonstrate a novel role of gut-derived 5-HT in shaping gut microbiota composition in relation to susceptibility to colitis, identifying 5-HT-microbiota axis as a potential new therapeutic target in intestinal inflammatory disorders.
30716420	4	11	theme	Gut	620:622	arg1	microbiota					624:633	METHODS Gut microbiota	612:633	METHODS Gut microbiota of Tph1-/- and Tph1+/- mice	612:661	METHODS Gut microbiota of Tph1-/- and Tph1+/- mice were investigated by deep sequencing.
30716420	3	12	theme	gut-derived	542:552	arg1	5-HT					554:557	gut-derived 5-HT	542:557	gut-derived 5-HT	542:557	Considering the close proximity of EC cells and the microbes, we investigated the influence of gut-derived 5-HT on the microbiota and the susceptibility to colitis.
30716420	6	13	from	influence	813:821	arg1	microbiota					834:843	microbiota	834:843	microbiota	834:843	The indirect influence of 5-HT on microbiota was assessed by measuring antimicrobial peptides, specifically β-defensins, in the colon of mice and HT-29 colonic epithelial cells.
30716420	7	14	theme	sodium-induced	1045:1058	arg1	colitis					1060:1066	dextran sulfate sodium-induced colitis	1029:1066	dextran sulfate sodium-induced colitis	1029:1066	The impact of gut microbiota on the development of dextran sulfate sodium-induced colitis was assessed by transferring gut microbiota from Tph1-/- mice to Tph1+/- littermates and vice versa, as well as in germ-free mice.
30716420	0	15	theme	Microbiota	18:27	arg1	Composition					29:39	Gut Microbiota Composition	14:39	Gut Microbiota Composition	14:39	Modulation of Gut Microbiota Composition by Serotonin Signaling Influences Intestinal Immune Response and Susceptibility to Colitis.
30716420	7	16	theme	microbiota	996:1005	arg1	impact					982:987	The impact	978:987	The impact of gut microbiota on the development of dextran sulfate sodium-induced colitis	978:1066	The impact of gut microbiota on the development of dextran sulfate sodium-induced colitis was assessed by transferring gut microbiota from Tph1-/- mice to Tph1+/- littermates and vice versa, as well as in germ-free mice.
30716420	11	17	from	mice	1642:1645	arg1	microbiota					1618:1627	microbiota	1618:1627	microbiota from Tph1-/- mice mediating the protective effects	1618:1678	Microbial transfer from Tph1-/- to Tph1+/- littermates and vice versa altered colitis severity, with microbiota from Tph1-/- mice mediating the protective effects.
30716420	7	18	theme	dextran	1029:1035	arg1	sulfate					1037:1043	dextran sulfate	1029:1043	dextran sulfate sodium-induced colitis	1029:1066	The impact of gut microbiota on the development of dextran sulfate sodium-induced colitis was assessed by transferring gut microbiota from Tph1-/- mice to Tph1+/- littermates and vice versa, as well as in germ-free mice.
30716420	8	19	from	difference	1221:1230	arg1	composition					1245:1255	microbial composition	1235:1255	microbial composition	1235:1255	RESULTS A significant difference in microbial composition between Tph1-/- and Tph1+/- littermates was observed.
30716420	1	20	theme	BACKGROUND	133:142	arg1	5-hydroxytryptamine					162:180	5-hydroxytryptamine [5-HT]	162:187	5-hydroxytryptamine [5-HT]	162:187	BACKGROUND & AIMS Serotonin (5-hydroxytryptamine [5-HT]) is synthesized mainly within enterochromaffin (EC) cells in the gut, and tryptophan hydroxylase 1 (Tph1) is the rate-limiting enzyme for 5-HT synthesis in EC cells.
30716420	1	20	theme	BACKGROUND	133:142	arg1	Serotonin					151:159	BACKGROUND & AIMS Serotonin	133:159	BACKGROUND & AIMS Serotonin (5-hydroxytryptamine [5-HT])	133:188	BACKGROUND & AIMS Serotonin (5-hydroxytryptamine [5-HT]) is synthesized mainly within enterochromaffin (EC) cells in the gut, and tryptophan hydroxylase 1 (Tph1) is the rate-limiting enzyme for 5-HT synthesis in EC cells.
30716420	12	21	theme	severe	1768:1773	arg1	colitis					1806:1812	dextran sulfate sodium-induced colitis	1775:1812	less severe dextran sulfate sodium-induced colitis	1763:1812	Furthermore, germ-free mice colonized with microbiota from Tph1-/- mice exhibited less severe dextran sulfate sodium-induced colitis.
30716420	7	22	theme	Tph1+/-	1133:1139	arg1	littermates					1141:1151	Tph1+/- littermates	1133:1151	Tph1+/- littermates	1133:1151	The impact of gut microbiota on the development of dextran sulfate sodium-induced colitis was assessed by transferring gut microbiota from Tph1-/- mice to Tph1+/- littermates and vice versa, as well as in germ-free mice.
30716420	9	23	theme	species-specific	1434:1449	arg1	effect					1451:1456	a concentration-dependent and species-specific effect	1404:1456	a concentration-dependent and species-specific effect	1404:1456	5-HT directly stimulated and inhibited the growth of commensal bacteria in vitro, exhibiting a concentration-dependent and species-specific effect.
30716420	1	24	theme	AIMS	146:149	arg1	5-hydroxytryptamine					162:180	5-hydroxytryptamine [5-HT]	162:187	5-hydroxytryptamine [5-HT]	162:187	BACKGROUND & AIMS Serotonin (5-hydroxytryptamine [5-HT]) is synthesized mainly within enterochromaffin (EC) cells in the gut, and tryptophan hydroxylase 1 (Tph1) is the rate-limiting enzyme for 5-HT synthesis in EC cells.
30716420	1	24	theme	AIMS	146:149	arg1	Serotonin					151:159	BACKGROUND & AIMS Serotonin	133:159	BACKGROUND & AIMS Serotonin (5-hydroxytryptamine [5-HT])	133:188	BACKGROUND & AIMS Serotonin (5-hydroxytryptamine [5-HT]) is synthesized mainly within enterochromaffin (EC) cells in the gut, and tryptophan hydroxylase 1 (Tph1) is the rate-limiting enzyme for 5-HT synthesis in EC cells.
30716420	12	25	theme	sulfate	1783:1789	arg1	colitis					1806:1812	dextran sulfate sodium-induced colitis	1775:1812	less severe dextran sulfate sodium-induced colitis	1763:1812	Furthermore, germ-free mice colonized with microbiota from Tph1-/- mice exhibited less severe dextran sulfate sodium-induced colitis.
30716420	11	26	theme	Microbial	1517:1525	arg1	transfer					1527:1534	Microbial transfer	1517:1534	Microbial transfer from Tph1-/- to Tph1+/- littermates	1517:1570	Microbial transfer from Tph1-/- to Tph1+/- littermates and vice versa altered colitis severity, with microbiota from Tph1-/- mice mediating the protective effects.
30716420	1	27	theme	rate-limiting	302:314	arg1	enzyme					316:321	the rate-limiting enzyme	298:321	the rate-limiting enzyme for 5-HT synthesis in EC cells	298:352	BACKGROUND & AIMS Serotonin (5-hydroxytryptamine [5-HT]) is synthesized mainly within enterochromaffin (EC) cells in the gut, and tryptophan hydroxylase 1 (Tph1) is the rate-limiting enzyme for 5-HT synthesis in EC cells.
30716420	1	27	theme	rate-limiting	302:314	arg1	hydroxylase					274:284	tryptophan hydroxylase 1	263:286	tryptophan hydroxylase 1 (Tph1)	263:293	BACKGROUND & AIMS Serotonin (5-hydroxytryptamine [5-HT]) is synthesized mainly within enterochromaffin (EC) cells in the gut, and tryptophan hydroxylase 1 (Tph1) is the rate-limiting enzyme for 5-HT synthesis in EC cells.
30716420	12	28	theme	Tph1-/-	1740:1746	arg1	mice					1748:1751	Tph1-/- mice	1740:1751	Tph1-/- mice	1740:1751	Furthermore, germ-free mice colonized with microbiota from Tph1-/- mice exhibited less severe dextran sulfate sodium-induced colitis.
30716420	10	29	theme	β-defensin	1479:1488	arg1	production					1490:1499	β-defensin production	1479:1499	β-defensin production	1479:1499	5-HT also inhibited β-defensin production by HT-29 cells.
30716420	4	30	theme	deep	684:687	arg1	sequencing					689:698	deep sequencing	684:698	deep sequencing	684:698	METHODS Gut microbiota of Tph1-/- and Tph1+/- mice were investigated by deep sequencing.
30716420	0	31	theme	Immune	86:91	arg1	Response					93:100	Intestinal Immune Response	75:100	Intestinal Immune Response	75:100	Modulation of Gut Microbiota Composition by Serotonin Signaling Influences Intestinal Immune Response and Susceptibility to Colitis.
30716420	3	32	from	influence	529:537	arg1	microbiota					566:575	the microbiota	562:575	the microbiota	562:575	Considering the close proximity of EC cells and the microbes, we investigated the influence of gut-derived 5-HT on the microbiota and the susceptibility to colitis.
30716420	5	33	theme	5-HT	721:724	arg1	influence					708:716	Direct influence	701:716	Direct influence of 5-HT on bacteria	701:736	Direct influence of 5-HT on bacteria was assessed by using in vitro system of isolated commensals.
30716420	13	34	link	gut-derived	1870:1880	arg1	5-HT					1882:1885	gut-derived 5-HT	1870:1885	gut-derived 5-HT	1870:1885	CONCLUSIONS These findings demonstrate a novel role of gut-derived 5-HT in shaping gut microbiota composition in relation to susceptibility to colitis, identifying 5-HT-microbiota axis as a potential new therapeutic target in intestinal inflammatory disorders.
30716420	6	35	theme	cells	971:975	arg1	colon					928:932	the colon	924:932	the colon of mice and HT-29 colonic epithelial cells	924:975	The indirect influence of 5-HT on microbiota was assessed by measuring antimicrobial peptides, specifically β-defensins, in the colon of mice and HT-29 colonic epithelial cells.
30716420	1	36	theme	EC	345:346	arg1	cells					348:352	EC cells	345:352	EC cells	345:352	BACKGROUND & AIMS Serotonin (5-hydroxytryptamine [5-HT]) is synthesized mainly within enterochromaffin (EC) cells in the gut, and tryptophan hydroxylase 1 (Tph1) is the rate-limiting enzyme for 5-HT synthesis in EC cells.
30716420	13	37	dep	CONCLUSIONS	1815:1825	arg1	demonstrate					1842:1852	demonstrate	1842:1852	demonstrate	1842:1852	CONCLUSIONS These findings demonstrate a novel role of gut-derived 5-HT in shaping gut microbiota composition in relation to susceptibility to colitis, identifying 5-HT-microbiota axis as a potential new therapeutic target in intestinal inflammatory disorders.
30716420	13	38	from	target	2031:2036	arg1	disorders					2065:2073	intestinal inflammatory disorders	2041:2073	intestinal inflammatory disorders	2041:2073	CONCLUSIONS These findings demonstrate a novel role of gut-derived 5-HT in shaping gut microbiota composition in relation to susceptibility to colitis, identifying 5-HT-microbiota axis as a potential new therapeutic target in intestinal inflammatory disorders.
30716420	2	39	theme	Accumulating	355:366	arg1	evidence					368:375	Accumulating evidence	355:375	Accumulating evidence	355:375	Accumulating evidence suggests the importance of gut microbiota in intestinal inflammation.
30716420	1	40	theme	tryptophan	263:272	arg1	hydroxylase					274:284	tryptophan hydroxylase 1	263:286	tryptophan hydroxylase 1 (Tph1)	263:293	BACKGROUND & AIMS Serotonin (5-hydroxytryptamine [5-HT]) is synthesized mainly within enterochromaffin (EC) cells in the gut, and tryptophan hydroxylase 1 (Tph1) is the rate-limiting enzyme for 5-HT synthesis in EC cells.
30716420	1	40	theme	tryptophan	263:272	arg1	Tph1					289:292	Tph1	289:292	Tph1	289:292	BACKGROUND & AIMS Serotonin (5-hydroxytryptamine [5-HT]) is synthesized mainly within enterochromaffin (EC) cells in the gut, and tryptophan hydroxylase 1 (Tph1) is the rate-limiting enzyme for 5-HT synthesis in EC cells.
30716420	1	40	theme	tryptophan	263:272	arg1	enzyme					316:321	the rate-limiting enzyme	298:321	the rate-limiting enzyme for 5-HT synthesis in EC cells	298:352	BACKGROUND & AIMS Serotonin (5-hydroxytryptamine [5-HT]) is synthesized mainly within enterochromaffin (EC) cells in the gut, and tryptophan hydroxylase 1 (Tph1) is the rate-limiting enzyme for 5-HT synthesis in EC cells.
30716420	2	41	theme	gut	404:406	arg1	microbiota					408:417	gut microbiota	404:417	gut microbiota	404:417	Accumulating evidence suggests the importance of gut microbiota in intestinal inflammation.
30716420	0	42	theme	Gut	14:16	arg1	Composition					29:39	Gut Microbiota Composition	14:39	Gut Microbiota Composition	14:39	Modulation of Gut Microbiota Composition by Serotonin Signaling Influences Intestinal Immune Response and Susceptibility to Colitis.
30716420	12	43	theme	germ-free	1694:1702	arg1	mice					1704:1707	germ-free mice	1694:1707	germ-free mice colonized with microbiota from Tph1-/- mice	1694:1751	Furthermore, germ-free mice colonized with microbiota from Tph1-/- mice exhibited less severe dextran sulfate sodium-induced colitis.
30716420	13	44	theme	new	2015:2017	arg1	axis					1995:1998	5-HT-microbiota axis	1979:1998	5-HT-microbiota axis	1979:1998	CONCLUSIONS These findings demonstrate a novel role of gut-derived 5-HT in shaping gut microbiota composition in relation to susceptibility to colitis, identifying 5-HT-microbiota axis as a potential new therapeutic target in intestinal inflammatory disorders.
30716420	13	44	theme	new	2015:2017	arg1	target					2031:2036	a potential new therapeutic target	2003:2036	a potential new therapeutic target in intestinal inflammatory disorders	2003:2073	CONCLUSIONS These findings demonstrate a novel role of gut-derived 5-HT in shaping gut microbiota composition in relation to susceptibility to colitis, identifying 5-HT-microbiota axis as a potential new therapeutic target in intestinal inflammatory disorders.
30716420	0	45	theme	Composition	29:39	arg1	Modulation					0:9	Modulation	0:9	Modulation of Gut Microbiota Composition by Serotonin Signaling	0:62	Modulation of Gut Microbiota Composition by Serotonin Signaling Influences Intestinal Immune Response and Susceptibility to Colitis.
30716420	3	46	theme	EC	482:483	arg1	cells					485:489	EC cells	482:489	EC cells	482:489	Considering the close proximity of EC cells and the microbes, we investigated the influence of gut-derived 5-HT on the microbiota and the susceptibility to colitis.
30716420	5	47	theme	isolated	779:786	arg1	commensals					788:797	isolated commensals	779:797	isolated commensals	779:797	Direct influence of 5-HT on bacteria was assessed by using in vitro system of isolated commensals.
30716420	9	48	theme	bacteria	1374:1381	arg1	growth					1354:1359	the growth	1350:1359	the growth of commensal bacteria	1350:1381	5-HT directly stimulated and inhibited the growth of commensal bacteria in vitro, exhibiting a concentration-dependent and species-specific effect.
30716420	13	49	from	composition	1913:1923	arg1	relation					1928:1935	relation	1928:1935	relation to susceptibility to colitis	1928:1964	CONCLUSIONS These findings demonstrate a novel role of gut-derived 5-HT in shaping gut microbiota composition in relation to susceptibility to colitis, identifying 5-HT-microbiota axis as a potential new therapeutic target in intestinal inflammatory disorders.
30716420	1	50	theme	EC	237:238	arg1	cells					241:245	enterochromaffin (EC) cells	219:245	enterochromaffin (EC) cells in the gut	219:256	BACKGROUND & AIMS Serotonin (5-hydroxytryptamine [5-HT]) is synthesized mainly within enterochromaffin (EC) cells in the gut, and tryptophan hydroxylase 1 (Tph1) is the rate-limiting enzyme for 5-HT synthesis in EC cells.
30716420	2	51	from	importance	390:399	arg1	inflammation					433:444	intestinal inflammation	422:444	intestinal inflammation	422:444	Accumulating evidence suggests the importance of gut microbiota in intestinal inflammation.
30716420	13	52	theme	5-HT	1882:1885	arg1	role					1862:1865	a novel role	1854:1865	a novel role of gut-derived 5-HT in shaping gut microbiota composition in relation to susceptibility to colitis	1854:1964	CONCLUSIONS These findings demonstrate a novel role of gut-derived 5-HT in shaping gut microbiota composition in relation to susceptibility to colitis, identifying 5-HT-microbiota axis as a potential new therapeutic target in intestinal inflammatory disorders.
30716420	3	53	theme	microbes	499:506	arg1	proximity					469:477	the close proximity	459:477	the close proximity of EC cells and the microbes	459:506	Considering the close proximity of EC cells and the microbes, we investigated the influence of gut-derived 5-HT on the microbiota and the susceptibility to colitis.
30716420	8	54	theme	significant	1209:1219	arg1	difference					1221:1230	A significant difference	1207:1230	A significant difference in microbial composition between Tph1-/- and Tph1+/- littermates	1207:1295	RESULTS A significant difference in microbial composition between Tph1-/- and Tph1+/- littermates was observed.
30716420	13	55	theme	shaping	1890:1896	arg1	composition					1913:1923	shaping gut microbiota composition	1890:1923	shaping gut microbiota composition in relation to susceptibility to colitis	1890:1964	CONCLUSIONS These findings demonstrate a novel role of gut-derived 5-HT in shaping gut microbiota composition in relation to susceptibility to colitis, identifying 5-HT-microbiota axis as a potential new therapeutic target in intestinal inflammatory disorders.
30716420	7	56	theme	Tph1-/-	1117:1123	arg1	mice					1125:1128	Tph1-/- mice	1117:1128	Tph1-/- mice	1117:1128	The impact of gut microbiota on the development of dextran sulfate sodium-induced colitis was assessed by transferring gut microbiota from Tph1-/- mice to Tph1+/- littermates and vice versa, as well as in germ-free mice.
30716420	6	57	theme	β-defensins	908:918	arg1	peptides					885:892	antimicrobial peptides	871:892	antimicrobial peptides	871:892	The indirect influence of 5-HT on microbiota was assessed by measuring antimicrobial peptides, specifically β-defensins, in the colon of mice and HT-29 colonic epithelial cells.
30716420	13	58	theme	novel	1856:1860	arg1	role					1862:1865	a novel role	1854:1865	a novel role of gut-derived 5-HT in shaping gut microbiota composition in relation to susceptibility to colitis	1854:1964	CONCLUSIONS These findings demonstrate a novel role of gut-derived 5-HT in shaping gut microbiota composition in relation to susceptibility to colitis, identifying 5-HT-microbiota axis as a potential new therapeutic target in intestinal inflammatory disorders.
30716420	4	59	theme	mice	658:661	arg1	microbiota					624:633	METHODS Gut microbiota	612:633	METHODS Gut microbiota of Tph1-/- and Tph1+/- mice	612:661	METHODS Gut microbiota of Tph1-/- and Tph1+/- mice were investigated by deep sequencing.
30716420	0	60	theme	Serotonin	44:52	arg1	Signaling					54:62	Serotonin Signaling	44:62	Serotonin Signaling	44:62	Modulation of Gut Microbiota Composition by Serotonin Signaling Influences Intestinal Immune Response and Susceptibility to Colitis.
30716420	6	61	theme	antimicrobial	871:883	arg1	peptides					885:892	antimicrobial peptides	871:892	antimicrobial peptides	871:892	The indirect influence of 5-HT on microbiota was assessed by measuring antimicrobial peptides, specifically β-defensins, in the colon of mice and HT-29 colonic epithelial cells.
30716420	4	62	theme	Tph1-/-	638:644	arg1	mice					658:661	Tph1-/- and Tph1+/- mice	638:661	Tph1-/- and Tph1+/- mice	638:661	METHODS Gut microbiota of Tph1-/- and Tph1+/- mice were investigated by deep sequencing.
30716420	3	63	theme	5-HT	554:557	arg1	influence					529:537	the influence	525:537	the influence of gut-derived 5-HT on the microbiota	525:575	Considering the close proximity of EC cells and the microbes, we investigated the influence of gut-derived 5-HT on the microbiota and the susceptibility to colitis.
30716420	3	63	theme	5-HT	554:557	arg1	susceptibility					585:598	the susceptibility	581:598	the susceptibility to colitis	581:609	Considering the close proximity of EC cells and the microbes, we investigated the influence of gut-derived 5-HT on the microbiota and the susceptibility to colitis.
30716420	6	64	theme	epithelial	960:969	arg1	cells					971:975	HT-29 colonic epithelial cells	946:975	HT-29 colonic epithelial cells	946:975	The indirect influence of 5-HT on microbiota was assessed by measuring antimicrobial peptides, specifically β-defensins, in the colon of mice and HT-29 colonic epithelial cells.
30716420	4	65	theme	Tph1+/-	650:656	arg1	mice					658:661	Tph1-/- and Tph1+/- mice	638:661	Tph1-/- and Tph1+/- mice	638:661	METHODS Gut microbiota of Tph1-/- and Tph1+/- mice were investigated by deep sequencing.
30716420	7	66	theme	colitis	1060:1066	arg1	development					1014:1024	the development	1010:1024	the development of dextran sulfate sodium-induced colitis	1010:1066	The impact of gut microbiota on the development of dextran sulfate sodium-induced colitis was assessed by transferring gut microbiota from Tph1-/- mice to Tph1+/- littermates and vice versa, as well as in germ-free mice.
30716420	13	67	theme	intestinal	2041:2050	arg1	disorders					2065:2073	intestinal inflammatory disorders	2041:2073	intestinal inflammatory disorders	2041:2073	CONCLUSIONS These findings demonstrate a novel role of gut-derived 5-HT in shaping gut microbiota composition in relation to susceptibility to colitis, identifying 5-HT-microbiota axis as a potential new therapeutic target in intestinal inflammatory disorders.
30716420	6	68	theme	HT-29	946:950	arg1	cells					971:975	HT-29 colonic epithelial cells	946:975	HT-29 colonic epithelial cells	946:975	The indirect influence of 5-HT on microbiota was assessed by measuring antimicrobial peptides, specifically β-defensins, in the colon of mice and HT-29 colonic epithelial cells.
30716420	4	69	theme	METHODS	612:618	arg1	microbiota					624:633	METHODS Gut microbiota	612:633	METHODS Gut microbiota of Tph1-/- and Tph1+/- mice	612:661	METHODS Gut microbiota of Tph1-/- and Tph1+/- mice were investigated by deep sequencing.
30716420	5	70	theme	Direct	701:706	arg1	influence					708:716	Direct influence	701:716	Direct influence of 5-HT on bacteria	701:736	Direct influence of 5-HT on bacteria was assessed by using in vitro system of isolated commensals.
30716420	7	71	from	impact	982:987	arg1	development					1014:1024	the development	1010:1024	the development of dextran sulfate sodium-induced colitis	1010:1066	The impact of gut microbiota on the development of dextran sulfate sodium-induced colitis was assessed by transferring gut microbiota from Tph1-/- mice to Tph1+/- littermates and vice versa, as well as in germ-free mice.
30716420	1	72	theme	enterochromaffin	219:234	arg1	cells					241:245	enterochromaffin (EC) cells	219:245	enterochromaffin (EC) cells in the gut	219:256	BACKGROUND & AIMS Serotonin (5-hydroxytryptamine [5-HT]) is synthesized mainly within enterochromaffin (EC) cells in the gut, and tryptophan hydroxylase 1 (Tph1) is the rate-limiting enzyme for 5-HT synthesis in EC cells.
30716420	6	73	theme	mice	937:940	arg1	colon					928:932	the colon	924:932	the colon of mice and HT-29 colonic epithelial cells	924:975	The indirect influence of 5-HT on microbiota was assessed by measuring antimicrobial peptides, specifically β-defensins, in the colon of mice and HT-29 colonic epithelial cells.
30716420	8	74	theme	Tph1-/-	1265:1271	arg1	littermates					1285:1295	Tph1-/- and Tph1+/- littermates	1265:1295	Tph1-/- and Tph1+/- littermates	1265:1295	RESULTS A significant difference in microbial composition between Tph1-/- and Tph1+/- littermates was observed.
30716420	1	75	from	cells	241:245	arg1	gut					254:256	the gut	250:256	the gut	250:256	BACKGROUND & AIMS Serotonin (5-hydroxytryptamine [5-HT]) is synthesized mainly within enterochromaffin (EC) cells in the gut, and tryptophan hydroxylase 1 (Tph1) is the rate-limiting enzyme for 5-HT synthesis in EC cells.
30716420	13	76	from	role	1862:1865	arg1	composition					1913:1923	shaping gut microbiota composition	1890:1923	shaping gut microbiota composition in relation to susceptibility to colitis	1890:1964	CONCLUSIONS These findings demonstrate a novel role of gut-derived 5-HT in shaping gut microbiota composition in relation to susceptibility to colitis, identifying 5-HT-microbiota axis as a potential new therapeutic target in intestinal inflammatory disorders.
30716420	8	77	theme	Tph1+/-	1277:1283	arg1	littermates					1285:1295	Tph1-/- and Tph1+/- littermates	1265:1295	Tph1-/- and Tph1+/- littermates	1265:1295	RESULTS A significant difference in microbial composition between Tph1-/- and Tph1+/- littermates was observed.
30716420	7	78	theme	gut	992:994	arg1	microbiota					996:1005	gut microbiota	992:1005	gut microbiota	992:1005	The impact of gut microbiota on the development of dextran sulfate sodium-induced colitis was assessed by transferring gut microbiota from Tph1-/- mice to Tph1+/- littermates and vice versa, as well as in germ-free mice.
30716420	1	79	theme	&	144:144	arg1	5-hydroxytryptamine					162:180	5-hydroxytryptamine [5-HT]	162:187	5-hydroxytryptamine [5-HT]	162:187	BACKGROUND & AIMS Serotonin (5-hydroxytryptamine [5-HT]) is synthesized mainly within enterochromaffin (EC) cells in the gut, and tryptophan hydroxylase 1 (Tph1) is the rate-limiting enzyme for 5-HT synthesis in EC cells.
30716420	1	79	theme	&	144:144	arg1	Serotonin					151:159	BACKGROUND & AIMS Serotonin	133:159	BACKGROUND & AIMS Serotonin (5-hydroxytryptamine [5-HT])	133:188	BACKGROUND & AIMS Serotonin (5-hydroxytryptamine [5-HT]) is synthesized mainly within enterochromaffin (EC) cells in the gut, and tryptophan hydroxylase 1 (Tph1) is the rate-limiting enzyme for 5-HT synthesis in EC cells.
30716420	13	80	theme	potential	2005:2013	arg1	axis					1995:1998	5-HT-microbiota axis	1979:1998	5-HT-microbiota axis	1979:1998	CONCLUSIONS These findings demonstrate a novel role of gut-derived 5-HT in shaping gut microbiota composition in relation to susceptibility to colitis, identifying 5-HT-microbiota axis as a potential new therapeutic target in intestinal inflammatory disorders.
30716420	13	80	theme	potential	2005:2013	arg1	target					2031:2036	a potential new therapeutic target	2003:2036	a potential new therapeutic target in intestinal inflammatory disorders	2003:2073	CONCLUSIONS These findings demonstrate a novel role of gut-derived 5-HT in shaping gut microbiota composition in relation to susceptibility to colitis, identifying 5-HT-microbiota axis as a potential new therapeutic target in intestinal inflammatory disorders.
30716420	0	81	theme	Intestinal	75:84	arg1	Response					93:100	Intestinal Immune Response	75:100	Intestinal Immune Response	75:100	Modulation of Gut Microbiota Composition by Serotonin Signaling Influences Intestinal Immune Response and Susceptibility to Colitis.
30716420	12	82	theme	dextran	1775:1781	arg1	sulfate					1783:1789	dextran sulfate	1775:1789	less severe dextran sulfate sodium-induced colitis	1763:1812	Furthermore, germ-free mice colonized with microbiota from Tph1-/- mice exhibited less severe dextran sulfate sodium-induced colitis.
30716420	3	83	link	gut-derived	542:552	arg1	5-HT					554:557	gut-derived 5-HT	542:557	gut-derived 5-HT	542:557	Considering the close proximity of EC cells and the microbes, we investigated the influence of gut-derived 5-HT on the microbiota and the susceptibility to colitis.
30716420	11	84	theme	Tph1+/-	1552:1558	arg1	littermates					1560:1570	Tph1+/- littermates	1552:1570	Tph1+/- littermates	1552:1570	Microbial transfer from Tph1-/- to Tph1+/- littermates and vice versa altered colitis severity, with microbiota from Tph1-/- mice mediating the protective effects.
30716420	7	85	theme	germ-free	1183:1191	arg1	mice					1193:1196	germ-free mice	1183:1196	germ-free mice	1183:1196	The impact of gut microbiota on the development of dextran sulfate sodium-induced colitis was assessed by transferring gut microbiota from Tph1-/- mice to Tph1+/- littermates and vice versa, as well as in germ-free mice.
30716420	13	86	theme	5-HT-microbiota	1979:1993	arg1	axis					1995:1998	5-HT-microbiota axis	1979:1998	5-HT-microbiota axis	1979:1998	CONCLUSIONS These findings demonstrate a novel role of gut-derived 5-HT in shaping gut microbiota composition in relation to susceptibility to colitis, identifying 5-HT-microbiota axis as a potential new therapeutic target in intestinal inflammatory disorders.
30716420	13	86	theme	5-HT-microbiota	1979:1993	arg1	target					2031:2036	a potential new therapeutic target	2003:2036	a potential new therapeutic target in intestinal inflammatory disorders	2003:2073	CONCLUSIONS These findings demonstrate a novel role of gut-derived 5-HT in shaping gut microbiota composition in relation to susceptibility to colitis, identifying 5-HT-microbiota axis as a potential new therapeutic target in intestinal inflammatory disorders.
30716420	1	87	theme	5-HT	327:330	arg1	synthesis					332:340	5-HT synthesis	327:340	5-HT synthesis in EC cells	327:352	BACKGROUND & AIMS Serotonin (5-hydroxytryptamine [5-HT]) is synthesized mainly within enterochromaffin (EC) cells in the gut, and tryptophan hydroxylase 1 (Tph1) is the rate-limiting enzyme for 5-HT synthesis in EC cells.
30716420	11	88	from	Tph1-/-	1541:1547	arg1	transfer					1527:1534	Microbial transfer	1517:1534	Microbial transfer from Tph1-/- to Tph1+/- littermates	1517:1570	Microbial transfer from Tph1-/- to Tph1+/- littermates and vice versa altered colitis severity, with microbiota from Tph1-/- mice mediating the protective effects.
30716420	3	89	from	susceptibility	585:598	arg1	microbiota					566:575	the microbiota	562:575	the microbiota	562:575	Considering the close proximity of EC cells and the microbes, we investigated the influence of gut-derived 5-HT on the microbiota and the susceptibility to colitis.
30716420	10	90	theme	HT-29	1504:1508	arg1	cells					1510:1514	HT-29 cells	1504:1514	HT-29 cells	1504:1514	5-HT also inhibited β-defensin production by HT-29 cells.
30716420	5	91	theme	commensals	788:797	arg1	system					769:774	in vitro system	760:774	in vitro system of isolated commensals	760:797	Direct influence of 5-HT on bacteria was assessed by using in vitro system of isolated commensals.
30716420	2	92	theme	intestinal	422:431	arg1	inflammation					433:444	intestinal inflammation	422:444	intestinal inflammation	422:444	Accumulating evidence suggests the importance of gut microbiota in intestinal inflammation.
30716420	13	93	theme	microbiota	1902:1911	arg1	composition					1913:1923	shaping gut microbiota composition	1890:1923	shaping gut microbiota composition in relation to susceptibility to colitis	1890:1964	CONCLUSIONS These findings demonstrate a novel role of gut-derived 5-HT in shaping gut microbiota composition in relation to susceptibility to colitis, identifying 5-HT-microbiota axis as a potential new therapeutic target in intestinal inflammatory disorders.
30716420	1	94	from	synthesis	332:340	arg1	cells					348:352	EC cells	345:352	EC cells	345:352	BACKGROUND & AIMS Serotonin (5-hydroxytryptamine [5-HT]) is synthesized mainly within enterochromaffin (EC) cells in the gut, and tryptophan hydroxylase 1 (Tph1) is the rate-limiting enzyme for 5-HT synthesis in EC cells.
30716420	2	95	theme	microbiota	408:417	arg1	importance					390:399	the importance	386:399	the importance of gut microbiota in intestinal inflammation	386:444	Accumulating evidence suggests the importance of gut microbiota in intestinal inflammation.
30716420	7	96	theme	sulfate	1037:1043	arg1	colitis					1060:1066	dextran sulfate sodium-induced colitis	1029:1066	dextran sulfate sodium-induced colitis	1029:1066	The impact of gut microbiota on the development of dextran sulfate sodium-induced colitis was assessed by transferring gut microbiota from Tph1-/- mice to Tph1+/- littermates and vice versa, as well as in germ-free mice.
30716420	8	97	dep	RESULTS	1199:1205	arg1	observed					1301:1308	observed	1301:1308	was observed	1297:1308	RESULTS A significant difference in microbial composition between Tph1-/- and Tph1+/- littermates was observed.
30716420	3	98	theme	close	463:467	arg1	proximity					469:477	the close proximity	459:477	the close proximity of EC cells and the microbes	459:506	Considering the close proximity of EC cells and the microbes, we investigated the influence of gut-derived 5-HT on the microbiota and the susceptibility to colitis.
30716420	5	99	theme	in vitro	760:767	arg1	system					769:774	in vitro system	760:774	in vitro system of isolated commensals	760:797	Direct influence of 5-HT on bacteria was assessed by using in vitro system of isolated commensals.
30716420	11	100	theme	Tph1-/-	1634:1640	arg1	mice					1642:1645	Tph1-/- mice	1634:1645	Tph1-/- mice mediating the protective effects	1634:1678	Microbial transfer from Tph1-/- to Tph1+/- littermates and vice versa altered colitis severity, with microbiota from Tph1-/- mice mediating the protective effects.
30716420	9	101	theme	commensal	1364:1372	arg1	bacteria					1374:1381	commensal bacteria	1364:1381	commensal bacteria	1364:1381	5-HT directly stimulated and inhibited the growth of commensal bacteria in vitro, exhibiting a concentration-dependent and species-specific effect.
30716420	3	102	theme	cells	485:489	arg1	proximity					469:477	the close proximity	459:477	the close proximity of EC cells and the microbes	459:506	Considering the close proximity of EC cells and the microbes, we investigated the influence of gut-derived 5-HT on the microbiota and the susceptibility to colitis.
30716420	6	103	theme	5-HT	826:829	arg1	influence					813:821	The indirect influence	800:821	The indirect influence of 5-HT on microbiota	800:843	The indirect influence of 5-HT on microbiota was assessed by measuring antimicrobial peptides, specifically β-defensins, in the colon of mice and HT-29 colonic epithelial cells.
30716420	11	104	theme	protective	1661:1670	arg1	effects					1672:1678	the protective effects	1657:1678	the protective effects	1657:1678	Microbial transfer from Tph1-/- to Tph1+/- littermates and vice versa altered colitis severity, with microbiota from Tph1-/- mice mediating the protective effects.
30716420	9	105	theme	concentration-dependent	1406:1428	arg1	effect					1451:1456	a concentration-dependent and species-specific effect	1404:1456	a concentration-dependent and species-specific effect	1404:1456	5-HT directly stimulated and inhibited the growth of commensal bacteria in vitro, exhibiting a concentration-dependent and species-specific effect.
29705317	3	0	theme	biphenyl	655:662	arg1	compounds					664:672	various biphenyl compounds	647:672	various biphenyl compounds	647:672	Catalytic performance of Pd NPs@CMC/AG was evaluated in synthesis of various biphenyl compounds by using the ultrasound-assisted method that was developed in this study.
29705317	2	1	theme	particles	537:545	arg1	size					525:528	size	525:528	size of the particles	525:545	Chemical characterization studies of Pd(0) nanoparticles (Pd NPs@CMC/AG) revealed that size of the particles were in the range of 37-55 nm.
29705317	5	2	theme	CMC/AG	995:1000	arg1	reproducibility					969:983	high reproducibility	964:983	high reproducibility of Pd NPs@CMC/AG	964:1000	In addition, Pd NPs@CMC/AG was successfully used up to six reaction cycles without losing its catalytic activity, indicating high reproducibility of Pd NPs@CMC/AG.
29705317	6	3	theme	reaction	1173:1180	arg1	time					1182:1185	shorter reaction time	1165:1185	shorter reaction time	1165:1185	Additionally, compared to conventional the methods, new ultrasound-assisted synthesis technique that was followed in this study exhibited some advantages such as shorter reaction time, greener reaction conditions, higher yields and easier work-up.
29705317	2	4	theme	characterization	447:462	arg1	studies					464:470	Chemical characterization studies	438:470	Chemical characterization studies of Pd(0) nanoparticles (Pd NPs@CMC/AG)	438:509	Chemical characterization studies of Pd(0) nanoparticles (Pd NPs@CMC/AG) revealed that size of the particles were in the range of 37-55 nm.
29705317	3	5	theme	@	609:609	arg1	CMC/AG					610:615	Pd NPs@CMC/AG	603:615	Pd NPs@CMC/AG	603:615	Catalytic performance of Pd NPs@CMC/AG was evaluated in synthesis of various biphenyl compounds by using the ultrasound-assisted method that was developed in this study.
29705317	4	6	theme	catalytic	782:790	arg1	performance					792:802	excellent catalytic performance	772:802	excellent catalytic performance	772:802	Pd NPs@CMC/AG exhibited excellent catalytic performance by producing high reaction yields.
29705317	1	7	theme	protocol	392:399	arg1	development					350:360	(ii) development	345:360	(ii) development of ultrasound assisted simple protocol for synthesis of biphenyl compounds	345:435	This study describes (i) an eco-friendly approach for design of Pd(0) nanoparticles on a natural composite, which is composed of carboxymethyl cellulose/agar polysaccharides (CMC/AG), without using any toxic reducing agents and (ii) development of ultrasound assisted simple protocol for synthesis of biphenyl compounds.
29705317	1	7	theme	protocol	392:399	arg1	agents					334:339	any toxic reducing agents	315:339	any toxic reducing agents	315:339	This study describes (i) an eco-friendly approach for design of Pd(0) nanoparticles on a natural composite, which is composed of carboxymethyl cellulose/agar polysaccharides (CMC/AG), without using any toxic reducing agents and (ii) development of ultrasound assisted simple protocol for synthesis of biphenyl compounds.
29705317	1	8	theme	eco-friendly	145:156	arg1	approach					158:165	an eco-friendly approach	142:165	(i) an eco-friendly approach for design of Pd(0) nanoparticles on a natural composite, which is composed of carboxymethyl cellulose/agar polysaccharides (CMC/AG)	138:298	This study describes (i) an eco-friendly approach for design of Pd(0) nanoparticles on a natural composite, which is composed of carboxymethyl cellulose/agar polysaccharides (CMC/AG), without using any toxic reducing agents and (ii) development of ultrasound assisted simple protocol for synthesis of biphenyl compounds.
29705317	2	9	theme	nanoparticles	481:493	arg1	studies					464:470	Chemical characterization studies	438:470	Chemical characterization studies of Pd(0) nanoparticles (Pd NPs@CMC/AG)	438:509	Chemical characterization studies of Pd(0) nanoparticles (Pd NPs@CMC/AG) revealed that size of the particles were in the range of 37-55 nm.
29705317	3	10	theme	CMC/AG	610:615	arg1	performance					588:598	Catalytic performance	578:598	Catalytic performance of Pd NPs@CMC/AG	578:615	Catalytic performance of Pd NPs@CMC/AG was evaluated in synthesis of various biphenyl compounds by using the ultrasound-assisted method that was developed in this study.
29705317	6	11	theme	greener	1188:1194	arg1	time					1182:1185	shorter reaction time	1165:1185	shorter reaction time	1165:1185	Additionally, compared to conventional the methods, new ultrasound-assisted synthesis technique that was followed in this study exhibited some advantages such as shorter reaction time, greener reaction conditions, higher yields and easier work-up.
29705317	6	11	theme	greener	1188:1194	arg1	conditions					1205:1214	greener reaction conditions	1188:1214	greener reaction conditions	1188:1214	Additionally, compared to conventional the methods, new ultrasound-assisted synthesis technique that was followed in this study exhibited some advantages such as shorter reaction time, greener reaction conditions, higher yields and easier work-up.
29705317	4	12	theme	NPs	751:753	arg1	CMC/AG					755:760	Pd NPs@CMC/AG	748:760	Pd NPs@CMC/AG	748:760	Pd NPs@CMC/AG exhibited excellent catalytic performance by producing high reaction yields.
29705317	5	13	theme	NPs	855:857	arg1	CMC/AG					859:864	Pd NPs@CMC/AG	852:864	Pd NPs@CMC/AG	852:864	In addition, Pd NPs@CMC/AG was successfully used up to six reaction cycles without losing its catalytic activity, indicating high reproducibility of Pd NPs@CMC/AG.
29705317	1	14	theme	toxic	319:323	arg1	agents					334:339	any toxic reducing agents	315:339	any toxic reducing agents	315:339	This study describes (i) an eco-friendly approach for design of Pd(0) nanoparticles on a natural composite, which is composed of carboxymethyl cellulose/agar polysaccharides (CMC/AG), without using any toxic reducing agents and (ii) development of ultrasound assisted simple protocol for synthesis of biphenyl compounds.
29705317	2	15	theme	37-55 nm	568:575	arg1	range					559:563	the range	555:563	the range of 37-55 nm	555:575	Chemical characterization studies of Pd(0) nanoparticles (Pd NPs@CMC/AG) revealed that size of the particles were in the range of 37-55 nm.
29705317	1	16	theme	reducing	325:332	arg1	agents					334:339	any toxic reducing agents	315:339	any toxic reducing agents	315:339	This study describes (i) an eco-friendly approach for design of Pd(0) nanoparticles on a natural composite, which is composed of carboxymethyl cellulose/agar polysaccharides (CMC/AG), without using any toxic reducing agents and (ii) development of ultrasound assisted simple protocol for synthesis of biphenyl compounds.
29705317	4	17	theme	@	754:754	arg1	CMC/AG					755:760	Pd NPs@CMC/AG	748:760	Pd NPs@CMC/AG	748:760	Pd NPs@CMC/AG exhibited excellent catalytic performance by producing high reaction yields.
29705317	1	18	theme	biphenyl	418:425	arg1	compounds					427:435	biphenyl compounds	418:435	biphenyl compounds	418:435	This study describes (i) an eco-friendly approach for design of Pd(0) nanoparticles on a natural composite, which is composed of carboxymethyl cellulose/agar polysaccharides (CMC/AG), without using any toxic reducing agents and (ii) development of ultrasound assisted simple protocol for synthesis of biphenyl compounds.
29705317	6	19	theme	synthesis	1079:1087	arg1	technique					1089:1097	new ultrasound-assisted synthesis technique	1055:1097	new ultrasound-assisted synthesis technique that was followed in this study	1055:1129	Additionally, compared to conventional the methods, new ultrasound-assisted synthesis technique that was followed in this study exhibited some advantages such as shorter reaction time, greener reaction conditions, higher yields and easier work-up.
29705317	3	20	theme	compounds	664:672	arg1	synthesis					634:642	synthesis	634:642	synthesis of various biphenyl compounds	634:672	Catalytic performance of Pd NPs@CMC/AG was evaluated in synthesis of various biphenyl compounds by using the ultrasound-assisted method that was developed in this study.
29705317	4	21	theme	excellent	772:780	arg1	performance					792:802	excellent catalytic performance	772:802	excellent catalytic performance	772:802	Pd NPs@CMC/AG exhibited excellent catalytic performance by producing high reaction yields.
29705317	1	22	theme	compounds	427:435	arg1	synthesis					405:413	synthesis	405:413	synthesis of biphenyl compounds	405:435	This study describes (i) an eco-friendly approach for design of Pd(0) nanoparticles on a natural composite, which is composed of carboxymethyl cellulose/agar polysaccharides (CMC/AG), without using any toxic reducing agents and (ii) development of ultrasound assisted simple protocol for synthesis of biphenyl compounds.
29705317	2	23	theme	Pd	496:497	arg1	CMC/AG					503:508	Pd NPs@CMC/AG	496:508	Pd NPs@CMC/AG	496:508	Chemical characterization studies of Pd(0) nanoparticles (Pd NPs@CMC/AG) revealed that size of the particles were in the range of 37-55 nm.
29705317	2	23	theme	Pd	496:497	arg1	nanoparticles					481:493	Pd(0) nanoparticles	475:493	Pd(0) nanoparticles (Pd NPs@CMC/AG)	475:509	Chemical characterization studies of Pd(0) nanoparticles (Pd NPs@CMC/AG) revealed that size of the particles were in the range of 37-55 nm.
29705317	3	24	theme	various	647:653	arg1	compounds					664:672	various biphenyl compounds	647:672	various biphenyl compounds	647:672	Catalytic performance of Pd NPs@CMC/AG was evaluated in synthesis of various biphenyl compounds by using the ultrasound-assisted method that was developed in this study.
29705317	0	25	theme	Ultrasound-accelerated	0:21	arg1	synthesis					23:31	Ultrasound-accelerated synthesis	0:31	Ultrasound-accelerated synthesis of biphenyl compounds using novel Pd(0) nanoparticles	0:85	Ultrasound-accelerated synthesis of biphenyl compounds using novel Pd(0) nanoparticles immobilized on bio-composite.
29705317	5	26	theme	@	858:858	arg1	CMC/AG					859:864	Pd NPs@CMC/AG	852:864	Pd NPs@CMC/AG	852:864	In addition, Pd NPs@CMC/AG was successfully used up to six reaction cycles without losing its catalytic activity, indicating high reproducibility of Pd NPs@CMC/AG.
29705317	5	27	theme	Pd	988:989	arg1	CMC/AG					995:1000	Pd NPs@CMC/AG	988:1000	Pd NPs@CMC/AG	988:1000	In addition, Pd NPs@CMC/AG was successfully used up to six reaction cycles without losing its catalytic activity, indicating high reproducibility of Pd NPs@CMC/AG.
29705317	0	28	theme	biphenyl	36:43	arg1	compounds					45:53	biphenyl compounds	36:53	biphenyl compounds using novel Pd(0) nanoparticles	36:85	Ultrasound-accelerated synthesis of biphenyl compounds using novel Pd(0) nanoparticles immobilized on bio-composite.
29705317	2	29	theme	@	502:502	arg1	CMC/AG					503:508	Pd NPs@CMC/AG	496:508	Pd NPs@CMC/AG	496:508	Chemical characterization studies of Pd(0) nanoparticles (Pd NPs@CMC/AG) revealed that size of the particles were in the range of 37-55 nm.
29705317	2	29	theme	@	502:502	arg1	nanoparticles					481:493	Pd(0) nanoparticles	475:493	Pd(0) nanoparticles (Pd NPs@CMC/AG)	475:509	Chemical characterization studies of Pd(0) nanoparticles (Pd NPs@CMC/AG) revealed that size of the particles were in the range of 37-55 nm.
29705317	4	30	theme	Pd	748:749	arg1	CMC/AG					755:760	Pd NPs@CMC/AG	748:760	Pd NPs@CMC/AG	748:760	Pd NPs@CMC/AG exhibited excellent catalytic performance by producing high reaction yields.
29705317	1	31	theme	Pd	181:182	arg1	nanoparticles					187:199	Pd(0) nanoparticles	181:199	Pd(0) nanoparticles on a natural composite, which is composed of carboxymethyl cellulose/agar polysaccharides (CMC/AG)	181:298	This study describes (i) an eco-friendly approach for design of Pd(0) nanoparticles on a natural composite, which is composed of carboxymethyl cellulose/agar polysaccharides (CMC/AG), without using any toxic reducing agents and (ii) development of ultrasound assisted simple protocol for synthesis of biphenyl compounds.
29705317	6	32	theme	new	1055:1057	arg1	technique					1089:1097	new ultrasound-assisted synthesis technique	1055:1097	new ultrasound-assisted synthesis technique that was followed in this study	1055:1129	Additionally, compared to conventional the methods, new ultrasound-assisted synthesis technique that was followed in this study exhibited some advantages such as shorter reaction time, greener reaction conditions, higher yields and easier work-up.
29705317	1	33	theme	carboxymethyl	246:258	arg1	CMC/AG					292:297	CMC/AG	292:297	CMC/AG	292:297	This study describes (i) an eco-friendly approach for design of Pd(0) nanoparticles on a natural composite, which is composed of carboxymethyl cellulose/agar polysaccharides (CMC/AG), without using any toxic reducing agents and (ii) development of ultrasound assisted simple protocol for synthesis of biphenyl compounds.
29705317	1	33	theme	carboxymethyl	246:258	arg1	polysaccharides					275:289	carboxymethyl cellulose/agar polysaccharides	246:289	carboxymethyl cellulose/agar polysaccharides (CMC/AG)	246:298	This study describes (i) an eco-friendly approach for design of Pd(0) nanoparticles on a natural composite, which is composed of carboxymethyl cellulose/agar polysaccharides (CMC/AG), without using any toxic reducing agents and (ii) development of ultrasound assisted simple protocol for synthesis of biphenyl compounds.
29705317	2	34	theme	NPs	499:501	arg1	CMC/AG					503:508	Pd NPs@CMC/AG	496:508	Pd NPs@CMC/AG	496:508	Chemical characterization studies of Pd(0) nanoparticles (Pd NPs@CMC/AG) revealed that size of the particles were in the range of 37-55 nm.
29705317	2	34	theme	NPs	499:501	arg1	nanoparticles					481:493	Pd(0) nanoparticles	475:493	Pd(0) nanoparticles (Pd NPs@CMC/AG)	475:509	Chemical characterization studies of Pd(0) nanoparticles (Pd NPs@CMC/AG) revealed that size of the particles were in the range of 37-55 nm.
29705317	5	35	theme	catalytic	933:941	arg1	activity					943:950	its catalytic activity	929:950	its catalytic activity	929:950	In addition, Pd NPs@CMC/AG was successfully used up to six reaction cycles without losing its catalytic activity, indicating high reproducibility of Pd NPs@CMC/AG.
29705317	2	36	theme	Chemical	438:445	arg1	studies					464:470	Chemical characterization studies	438:470	Chemical characterization studies of Pd(0) nanoparticles (Pd NPs@CMC/AG)	438:509	Chemical characterization studies of Pd(0) nanoparticles (Pd NPs@CMC/AG) revealed that size of the particles were in the range of 37-55 nm.
29705317	1	37	theme	cellulose/agar	260:273	arg1	CMC/AG					292:297	CMC/AG	292:297	CMC/AG	292:297	This study describes (i) an eco-friendly approach for design of Pd(0) nanoparticles on a natural composite, which is composed of carboxymethyl cellulose/agar polysaccharides (CMC/AG), without using any toxic reducing agents and (ii) development of ultrasound assisted simple protocol for synthesis of biphenyl compounds.
29705317	1	37	theme	cellulose/agar	260:273	arg1	polysaccharides					275:289	carboxymethyl cellulose/agar polysaccharides	246:289	carboxymethyl cellulose/agar polysaccharides (CMC/AG)	246:298	This study describes (i) an eco-friendly approach for design of Pd(0) nanoparticles on a natural composite, which is composed of carboxymethyl cellulose/agar polysaccharides (CMC/AG), without using any toxic reducing agents and (ii) development of ultrasound assisted simple protocol for synthesis of biphenyl compounds.
29705317	1	38	from	composite	214:222	arg1	design					171:176	design	171:176	design of Pd(0) nanoparticles on a natural composite, which is composed of carboxymethyl cellulose/agar polysaccharides (CMC/AG)	171:298	This study describes (i) an eco-friendly approach for design of Pd(0) nanoparticles on a natural composite, which is composed of carboxymethyl cellulose/agar polysaccharides (CMC/AG), without using any toxic reducing agents and (ii) development of ultrasound assisted simple protocol for synthesis of biphenyl compounds.
29705317	3	39	theme	Catalytic	578:586	arg1	performance					588:598	Catalytic performance	578:598	Catalytic performance of Pd NPs@CMC/AG	578:615	Catalytic performance of Pd NPs@CMC/AG was evaluated in synthesis of various biphenyl compounds by using the ultrasound-assisted method that was developed in this study.
29705317	5	40	used	used	883:886	arg2	CMC/AG					859:864	Pd NPs@CMC/AG	852:864	Pd NPs@CMC/AG	852:864	In addition, Pd NPs@CMC/AG was successfully used up to six reaction cycles without losing its catalytic activity, indicating high reproducibility of Pd NPs@CMC/AG.
29705317	0	41	theme	compounds	45:53	arg1	synthesis					23:31	Ultrasound-accelerated synthesis	0:31	Ultrasound-accelerated synthesis of biphenyl compounds using novel Pd(0) nanoparticles	0:85	Ultrasound-accelerated synthesis of biphenyl compounds using novel Pd(0) nanoparticles immobilized on bio-composite.
29705317	6	42	theme	reaction	1196:1203	arg1	time					1182:1185	shorter reaction time	1165:1185	shorter reaction time	1165:1185	Additionally, compared to conventional the methods, new ultrasound-assisted synthesis technique that was followed in this study exhibited some advantages such as shorter reaction time, greener reaction conditions, higher yields and easier work-up.
29705317	6	42	theme	reaction	1196:1203	arg1	conditions					1205:1214	greener reaction conditions	1188:1214	greener reaction conditions	1188:1214	Additionally, compared to conventional the methods, new ultrasound-assisted synthesis technique that was followed in this study exhibited some advantages such as shorter reaction time, greener reaction conditions, higher yields and easier work-up.
29705317	6	43	theme	easier	1235:1240	arg1	work-up					1242:1248	easier work-up	1235:1248	easier work-up	1235:1248	Additionally, compared to conventional the methods, new ultrasound-assisted synthesis technique that was followed in this study exhibited some advantages such as shorter reaction time, greener reaction conditions, higher yields and easier work-up.
29705317	5	44	theme	NPs	991:993	arg1	CMC/AG					995:1000	Pd NPs@CMC/AG	988:1000	Pd NPs@CMC/AG	988:1000	In addition, Pd NPs@CMC/AG was successfully used up to six reaction cycles without losing its catalytic activity, indicating high reproducibility of Pd NPs@CMC/AG.
29705317	0	45	theme	Pd	67:68	arg1	nanoparticles					73:85	novel Pd(0) nanoparticles	61:85	novel Pd(0) nanoparticles	61:85	Ultrasound-accelerated synthesis of biphenyl compounds using novel Pd(0) nanoparticles immobilized on bio-composite.
29705317	4	46	theme	reaction	822:829	arg1	yields					831:836	high reaction yields	817:836	high reaction yields	817:836	Pd NPs@CMC/AG exhibited excellent catalytic performance by producing high reaction yields.
29705317	5	47	theme	@	994:994	arg1	CMC/AG					995:1000	Pd NPs@CMC/AG	988:1000	Pd NPs@CMC/AG	988:1000	In addition, Pd NPs@CMC/AG was successfully used up to six reaction cycles without losing its catalytic activity, indicating high reproducibility of Pd NPs@CMC/AG.
29705317	1	48	dep	approach	158:165	arg1	i					139:139	i	139:139	i	139:139	This study describes (i) an eco-friendly approach for design of Pd(0) nanoparticles on a natural composite, which is composed of carboxymethyl cellulose/agar polysaccharides (CMC/AG), without using any toxic reducing agents and (ii) development of ultrasound assisted simple protocol for synthesis of biphenyl compounds.
29705317	6	49	theme	higher	1217:1222	arg1	yields					1224:1229	higher yields	1217:1229	higher yields	1217:1229	Additionally, compared to conventional the methods, new ultrasound-assisted synthesis technique that was followed in this study exhibited some advantages such as shorter reaction time, greener reaction conditions, higher yields and easier work-up.
29705317	6	49	theme	higher	1217:1222	arg1	time					1182:1185	shorter reaction time	1165:1185	shorter reaction time	1165:1185	Additionally, compared to conventional the methods, new ultrasound-assisted synthesis technique that was followed in this study exhibited some advantages such as shorter reaction time, greener reaction conditions, higher yields and easier work-up.
29705317	0	50	theme	novel	61:65	arg1	nanoparticles					73:85	novel Pd(0) nanoparticles	61:85	novel Pd(0) nanoparticles	61:85	Ultrasound-accelerated synthesis of biphenyl compounds using novel Pd(0) nanoparticles immobilized on bio-composite.
29705317	4	51	theme	high	817:820	arg1	yields					831:836	high reaction yields	817:836	high reaction yields	817:836	Pd NPs@CMC/AG exhibited excellent catalytic performance by producing high reaction yields.
29705317	3	52	theme	Pd	603:604	arg1	CMC/AG					610:615	Pd NPs@CMC/AG	603:615	Pd NPs@CMC/AG	603:615	Catalytic performance of Pd NPs@CMC/AG was evaluated in synthesis of various biphenyl compounds by using the ultrasound-assisted method that was developed in this study.
29705317	3	53	theme	NPs	606:608	arg1	CMC/AG					610:615	Pd NPs@CMC/AG	603:615	Pd NPs@CMC/AG	603:615	Catalytic performance of Pd NPs@CMC/AG was evaluated in synthesis of various biphenyl compounds by using the ultrasound-assisted method that was developed in this study.
29705317	6	54	theme	conventional	1029:1040	arg1	methods					1046:1052	the methods	1042:1052	conventional the methods	1029:1052	Additionally, compared to conventional the methods, new ultrasound-assisted synthesis technique that was followed in this study exhibited some advantages such as shorter reaction time, greener reaction conditions, higher yields and easier work-up.
29705317	3	55	theme	ultrasound-assisted	687:705	arg1	method					707:712	the ultrasound-assisted method	683:712	the ultrasound-assisted method that was developed in this study	683:745	Catalytic performance of Pd NPs@CMC/AG was evaluated in synthesis of various biphenyl compounds by using the ultrasound-assisted method that was developed in this study.
29705317	1	56	from	nanoparticles	187:199	arg1	composite					214:222	a natural composite	204:222	a natural composite	204:222	This study describes (i) an eco-friendly approach for design of Pd(0) nanoparticles on a natural composite, which is composed of carboxymethyl cellulose/agar polysaccharides (CMC/AG), without using any toxic reducing agents and (ii) development of ultrasound assisted simple protocol for synthesis of biphenyl compounds.
29705317	1	57	theme	ultrasound	365:374	arg1	protocol					392:399	ultrasound assisted simple protocol	365:399	ultrasound assisted simple protocol for synthesis of biphenyl compounds	365:435	This study describes (i) an eco-friendly approach for design of Pd(0) nanoparticles on a natural composite, which is composed of carboxymethyl cellulose/agar polysaccharides (CMC/AG), without using any toxic reducing agents and (ii) development of ultrasound assisted simple protocol for synthesis of biphenyl compounds.
29705317	6	58	theme	shorter	1165:1171	arg1	time					1182:1185	shorter reaction time	1165:1185	shorter reaction time	1165:1185	Additionally, compared to conventional the methods, new ultrasound-assisted synthesis technique that was followed in this study exhibited some advantages such as shorter reaction time, greener reaction conditions, higher yields and easier work-up.
29705317	1	59	theme	nanoparticles	187:199	arg1	design					171:176	design	171:176	design of Pd(0) nanoparticles on a natural composite, which is composed of carboxymethyl cellulose/agar polysaccharides (CMC/AG)	171:298	This study describes (i) an eco-friendly approach for design of Pd(0) nanoparticles on a natural composite, which is composed of carboxymethyl cellulose/agar polysaccharides (CMC/AG), without using any toxic reducing agents and (ii) development of ultrasound assisted simple protocol for synthesis of biphenyl compounds.
29705317	1	60	theme	assisted	376:383	arg1	protocol					392:399	ultrasound assisted simple protocol	365:399	ultrasound assisted simple protocol for synthesis of biphenyl compounds	365:435	This study describes (i) an eco-friendly approach for design of Pd(0) nanoparticles on a natural composite, which is composed of carboxymethyl cellulose/agar polysaccharides (CMC/AG), without using any toxic reducing agents and (ii) development of ultrasound assisted simple protocol for synthesis of biphenyl compounds.
29705317	5	61	theme	reaction	898:905	arg1	cycles					907:912	up to six reaction cycles	888:912	up to six reaction cycles	888:912	In addition, Pd NPs@CMC/AG was successfully used up to six reaction cycles without losing its catalytic activity, indicating high reproducibility of Pd NPs@CMC/AG.
29705317	1	62	from	design	171:176	arg1	composite					214:222	a natural composite	204:222	a natural composite	204:222	This study describes (i) an eco-friendly approach for design of Pd(0) nanoparticles on a natural composite, which is composed of carboxymethyl cellulose/agar polysaccharides (CMC/AG), without using any toxic reducing agents and (ii) development of ultrasound assisted simple protocol for synthesis of biphenyl compounds.
29705317	5	63	theme	Pd	852:853	arg1	CMC/AG					859:864	Pd NPs@CMC/AG	852:864	Pd NPs@CMC/AG	852:864	In addition, Pd NPs@CMC/AG was successfully used up to six reaction cycles without losing its catalytic activity, indicating high reproducibility of Pd NPs@CMC/AG.
29705317	5	64	theme	high	964:967	arg1	reproducibility					969:983	high reproducibility	964:983	high reproducibility of Pd NPs@CMC/AG	964:1000	In addition, Pd NPs@CMC/AG was successfully used up to six reaction cycles without losing its catalytic activity, indicating high reproducibility of Pd NPs@CMC/AG.
29705317	1	65	dep	development	350:360	arg1	ii					346:347	ii	346:347	ii	346:347	This study describes (i) an eco-friendly approach for design of Pd(0) nanoparticles on a natural composite, which is composed of carboxymethyl cellulose/agar polysaccharides (CMC/AG), without using any toxic reducing agents and (ii) development of ultrasound assisted simple protocol for synthesis of biphenyl compounds.
29705317	2	66	theme	Pd	475:476	arg1	CMC/AG					503:508	Pd NPs@CMC/AG	496:508	Pd NPs@CMC/AG	496:508	Chemical characterization studies of Pd(0) nanoparticles (Pd NPs@CMC/AG) revealed that size of the particles were in the range of 37-55 nm.
29705317	2	66	theme	Pd	475:476	arg1	nanoparticles					481:493	Pd(0) nanoparticles	475:493	Pd(0) nanoparticles (Pd NPs@CMC/AG)	475:509	Chemical characterization studies of Pd(0) nanoparticles (Pd NPs@CMC/AG) revealed that size of the particles were in the range of 37-55 nm.
29705317	1	67	theme	simple	385:390	arg1	protocol					392:399	ultrasound assisted simple protocol	365:399	ultrasound assisted simple protocol for synthesis of biphenyl compounds	365:435	This study describes (i) an eco-friendly approach for design of Pd(0) nanoparticles on a natural composite, which is composed of carboxymethyl cellulose/agar polysaccharides (CMC/AG), without using any toxic reducing agents and (ii) development of ultrasound assisted simple protocol for synthesis of biphenyl compounds.
29705317	6	68	theme	ultrasound-assisted	1059:1077	arg1	technique					1089:1097	new ultrasound-assisted synthesis technique	1055:1097	new ultrasound-assisted synthesis technique that was followed in this study	1055:1129	Additionally, compared to conventional the methods, new ultrasound-assisted synthesis technique that was followed in this study exhibited some advantages such as shorter reaction time, greener reaction conditions, higher yields and easier work-up.
29705317	5	69	dep	six	894:896	arg1	to					891:892	to	891:892	to	891:892	In addition, Pd NPs@CMC/AG was successfully used up to six reaction cycles without losing its catalytic activity, indicating high reproducibility of Pd NPs@CMC/AG.
29705317	1	70	theme	natural	206:212	arg1	composite					214:222	a natural composite	204:222	a natural composite	204:222	This study describes (i) an eco-friendly approach for design of Pd(0) nanoparticles on a natural composite, which is composed of carboxymethyl cellulose/agar polysaccharides (CMC/AG), without using any toxic reducing agents and (ii) development of ultrasound assisted simple protocol for synthesis of biphenyl compounds.
31456169	5	0	theme	biofilms	1116:1123	arg1	combat					1106:1111	combat	1106:1111	combat	1106:1111	These results contribute to increase the knowledge of the composition and architecture of the extracellular matrix of biofilms of S. aureus, as well as evidence that the investigation of alternative products to assist in the control and combat of biofilms is a promising strategy.
31456169	5	0	theme	biofilms	1116:1123	arg1	control					1094:1100	control	1094:1100	control	1094:1100	These results contribute to increase the knowledge of the composition and architecture of the extracellular matrix of biofilms of S. aureus, as well as evidence that the investigation of alternative products to assist in the control and combat of biofilms is a promising strategy.
31456169	1	1	theme	contamination	151:163	arg1	episodes					165:172	food contamination episodes	146:172	food contamination episodes	146:172	Bacterial biofilms are involved in various medical infections and food contamination episodes and, for this reason, it is of great importance to developing new strategies of its prevention and control.
31456169	2	2	theme	nisin	317:321	arg1	concentration					300:312	The subinhibitory concentration	282:312	The subinhibitory concentration of nisin	282:321	The subinhibitory concentration of nisin was determined, and its effect against Staphylococcus aureus and Staphylococcus epidermidis biofilms was evaluated.
31456169	1	3	theme	new	236:238	arg1	strategies					240:249	new strategies	236:249	new strategies of its prevention and control	236:279	Bacterial biofilms are involved in various medical infections and food contamination episodes and, for this reason, it is of great importance to developing new strategies of its prevention and control.
31456169	0	4	from	Changes	0:6	arg1	architecture					31:42	architecture	31:42	architecture	31:42	Changes in the composition and architecture of staphylococcal biofilm by nisin.
31456169	0	4	from	Changes	0:6	arg1	composition					15:25	composition	15:25	composition	15:25	Changes in the composition and architecture of staphylococcal biofilm by nisin.
31456169	3	5	theme	biofilms	560:567	arg1	structure					547:555	the structure	543:555	the structure of biofilms	543:567	Results obtained by confocal laser microscopy demonstrated morphological changes in the architecture of the structure of biofilms.
31456169	5	6	theme	aureus	1002:1007	arg1	biofilms					987:994	biofilms	987:994	biofilms of S. aureus	987:1007	These results contribute to increase the knowledge of the composition and architecture of the extracellular matrix of biofilms of S. aureus, as well as evidence that the investigation of alternative products to assist in the control and combat of biofilms is a promising strategy.
31456169	4	7	theme	staphylococcal	740:753	arg1	biofilms					755:762	staphylococcal biofilms	740:762	staphylococcal biofilms	740:762	The main components (polysaccharides, proteins, and extracellular DNA (eDNA)) of the biofilm matrix were determined by spectrophotometry and showed that the formation of staphylococcal biofilms in the presence of nisin results in a less dense matrix structure with modification in its constituents.
31456169	4	8	dep	components	579:588	arg1	proteins					608:615	proteins	608:615	proteins	608:615	The main components (polysaccharides, proteins, and extracellular DNA (eDNA)) of the biofilm matrix were determined by spectrophotometry and showed that the formation of staphylococcal biofilms in the presence of nisin results in a less dense matrix structure with modification in its constituents.
31456169	4	8	dep	components	579:588	arg1	polysaccharides					591:605	polysaccharides	591:605	polysaccharides	591:605	The main components (polysaccharides, proteins, and extracellular DNA (eDNA)) of the biofilm matrix were determined by spectrophotometry and showed that the formation of staphylococcal biofilms in the presence of nisin results in a less dense matrix structure with modification in its constituents.
31456169	4	8	dep	components	579:588	arg1	DNA					636:638	extracellular DNA	622:638	extracellular DNA (eDNA)	622:645	The main components (polysaccharides, proteins, and extracellular DNA (eDNA)) of the biofilm matrix were determined by spectrophotometry and showed that the formation of staphylococcal biofilms in the presence of nisin results in a less dense matrix structure with modification in its constituents.
31456169	4	8	dep	components	579:588	arg1	eDNA					641:644	eDNA	641:644	eDNA	641:644	The main components (polysaccharides, proteins, and extracellular DNA (eDNA)) of the biofilm matrix were determined by spectrophotometry and showed that the formation of staphylococcal biofilms in the presence of nisin results in a less dense matrix structure with modification in its constituents.
31456169	3	9	theme	structure	547:555	arg1	architecture					527:538	the architecture	523:538	the architecture of the structure of biofilms	523:567	Results obtained by confocal laser microscopy demonstrated morphological changes in the architecture of the structure of biofilms.
31456169	5	10	theme	composition	927:937	arg1	knowledge					910:918	the knowledge	906:918	the knowledge of the composition and architecture of the extracellular matrix of biofilms of S. aureus	906:1007	These results contribute to increase the knowledge of the composition and architecture of the extracellular matrix of biofilms of S. aureus, as well as evidence that the investigation of alternative products to assist in the control and combat of biofilms is a promising strategy.
31456169	4	11	theme	biofilm	655:661	arg1	matrix					663:668	the biofilm matrix	651:668	the biofilm matrix	651:668	The main components (polysaccharides, proteins, and extracellular DNA (eDNA)) of the biofilm matrix were determined by spectrophotometry and showed that the formation of staphylococcal biofilms in the presence of nisin results in a less dense matrix structure with modification in its constituents.
31456169	4	12	with	structure	820:828	arg1	modification					835:846	modification	835:846	modification in its constituents	835:866	The main components (polysaccharides, proteins, and extracellular DNA (eDNA)) of the biofilm matrix were determined by spectrophotometry and showed that the formation of staphylococcal biofilms in the presence of nisin results in a less dense matrix structure with modification in its constituents.
31456169	2	13	theme	aureus	377:382	arg1	biofilms					415:422	Staphylococcus aureus and Staphylococcus epidermidis biofilms	362:422	Staphylococcus aureus and Staphylococcus epidermidis biofilms	362:422	The subinhibitory concentration of nisin was determined, and its effect against Staphylococcus aureus and Staphylococcus epidermidis biofilms was evaluated.
31456169	4	14	from	modification	835:846	arg1	constituents					855:866	its constituents	851:866	its constituents	851:866	The main components (polysaccharides, proteins, and extracellular DNA (eDNA)) of the biofilm matrix were determined by spectrophotometry and showed that the formation of staphylococcal biofilms in the presence of nisin results in a less dense matrix structure with modification in its constituents.
31456169	1	15	theme	Bacterial	80:88	arg1	biofilms					90:97	Bacterial biofilms	80:97	Bacterial biofilms	80:97	Bacterial biofilms are involved in various medical infections and food contamination episodes and, for this reason, it is of great importance to developing new strategies of its prevention and control.
31456169	4	16	theme	main	574:577	arg1	components					579:588	The main components	570:588	The main components (polysaccharides, proteins, and extracellular DNA (eDNA)) of the biofilm matrix	570:668	The main components (polysaccharides, proteins, and extracellular DNA (eDNA)) of the biofilm matrix were determined by spectrophotometry and showed that the formation of staphylococcal biofilms in the presence of nisin results in a less dense matrix structure with modification in its constituents.
31456169	5	17	theme	alternative	1056:1066	arg1	products					1068:1075	alternative products	1056:1075	alternative products	1056:1075	These results contribute to increase the knowledge of the composition and architecture of the extracellular matrix of biofilms of S. aureus, as well as evidence that the investigation of alternative products to assist in the control and combat of biofilms is a promising strategy.
31456169	3	18	from	changes	512:518	arg1	architecture					527:538	the architecture	523:538	the architecture of the structure of biofilms	523:567	Results obtained by confocal laser microscopy demonstrated morphological changes in the architecture of the structure of biofilms.
31456169	2	19	theme	subinhibitory	286:298	arg1	concentration					300:312	The subinhibitory concentration	282:312	The subinhibitory concentration of nisin	282:321	The subinhibitory concentration of nisin was determined, and its effect against Staphylococcus aureus and Staphylococcus epidermidis biofilms was evaluated.
31456169	4	20	theme	matrix	663:668	arg1	components					579:588	The main components	570:588	The main components (polysaccharides, proteins, and extracellular DNA (eDNA)) of the biofilm matrix	570:668	The main components (polysaccharides, proteins, and extracellular DNA (eDNA)) of the biofilm matrix were determined by spectrophotometry and showed that the formation of staphylococcal biofilms in the presence of nisin results in a less dense matrix structure with modification in its constituents.
31456169	1	21	theme	prevention	258:267	arg1	strategies					240:249	new strategies	236:249	new strategies of its prevention and control	236:279	Bacterial biofilms are involved in various medical infections and food contamination episodes and, for this reason, it is of great importance to developing new strategies of its prevention and control.
31456169	3	22	theme	laser	468:472	arg1	microscopy					474:483	confocal laser microscopy	459:483	confocal laser microscopy	459:483	Results obtained by confocal laser microscopy demonstrated morphological changes in the architecture of the structure of biofilms.
31456169	4	23	theme	nisin	783:787	arg1	presence					771:778	the presence	767:778	the presence of nisin	767:787	The main components (polysaccharides, proteins, and extracellular DNA (eDNA)) of the biofilm matrix were determined by spectrophotometry and showed that the formation of staphylococcal biofilms in the presence of nisin results in a less dense matrix structure with modification in its constituents.
31456169	5	24	theme	products	1068:1075	arg1	investigation					1039:1051	the investigation	1035:1051	the investigation of alternative products to assist in the control and combat of biofilms	1035:1123	These results contribute to increase the knowledge of the composition and architecture of the extracellular matrix of biofilms of S. aureus, as well as evidence that the investigation of alternative products to assist in the control and combat of biofilms is a promising strategy.
31456169	5	24	theme	products	1068:1075	arg1	strategy					1140:1147	a promising strategy	1128:1147	a promising strategy	1128:1147	These results contribute to increase the knowledge of the composition and architecture of the extracellular matrix of biofilms of S. aureus, as well as evidence that the investigation of alternative products to assist in the control and combat of biofilms is a promising strategy.
31456169	4	25	theme	biofilms	755:762	arg1	formation					727:735	the formation	723:735	the formation of staphylococcal biofilms in the presence of nisin	723:787	The main components (polysaccharides, proteins, and extracellular DNA (eDNA)) of the biofilm matrix were determined by spectrophotometry and showed that the formation of staphylococcal biofilms in the presence of nisin results in a less dense matrix structure with modification in its constituents.
31456169	1	26	theme	control	273:279	arg1	strategies					240:249	new strategies	236:249	new strategies of its prevention and control	236:279	Bacterial biofilms are involved in various medical infections and food contamination episodes and, for this reason, it is of great importance to developing new strategies of its prevention and control.
31456169	4	27	theme	matrix	813:818	arg1	structure					820:828	a less dense matrix structure	800:828	a less dense matrix structure with modification in its constituents	800:866	The main components (polysaccharides, proteins, and extracellular DNA (eDNA)) of the biofilm matrix were determined by spectrophotometry and showed that the formation of staphylococcal biofilms in the presence of nisin results in a less dense matrix structure with modification in its constituents.
31456169	2	28	theme	epidermidis	403:413	arg1	biofilms					415:422	Staphylococcus aureus and Staphylococcus epidermidis biofilms	362:422	Staphylococcus aureus and Staphylococcus epidermidis biofilms	362:422	The subinhibitory concentration of nisin was determined, and its effect against Staphylococcus aureus and Staphylococcus epidermidis biofilms was evaluated.
31456169	5	29	theme	promising	1130:1138	arg1	strategy					1140:1147	a promising strategy	1128:1147	a promising strategy	1128:1147	These results contribute to increase the knowledge of the composition and architecture of the extracellular matrix of biofilms of S. aureus, as well as evidence that the investigation of alternative products to assist in the control and combat of biofilms is a promising strategy.
31456169	5	29	theme	promising	1130:1138	arg1	investigation					1039:1051	the investigation	1035:1051	the investigation of alternative products to assist in the control and combat of biofilms	1035:1123	These results contribute to increase the knowledge of the composition and architecture of the extracellular matrix of biofilms of S. aureus, as well as evidence that the investigation of alternative products to assist in the control and combat of biofilms is a promising strategy.
31456169	4	30	theme	dense	807:811	arg1	structure					820:828	a less dense matrix structure	800:828	a less dense matrix structure with modification in its constituents	800:866	The main components (polysaccharides, proteins, and extracellular DNA (eDNA)) of the biofilm matrix were determined by spectrophotometry and showed that the formation of staphylococcal biofilms in the presence of nisin results in a less dense matrix structure with modification in its constituents.
31456169	3	31	theme	confocal	459:466	arg1	microscopy					474:483	confocal laser microscopy	459:483	confocal laser microscopy	459:483	Results obtained by confocal laser microscopy demonstrated morphological changes in the architecture of the structure of biofilms.
31456169	0	32	theme	staphylococcal	47:60	arg1	biofilm					62:68	staphylococcal biofilm	47:68	staphylococcal biofilm	47:68	Changes in the composition and architecture of staphylococcal biofilm by nisin.
31456169	5	33	dep	composition	927:937	arg1	the					923:925	the	923:925	the	923:925	These results contribute to increase the knowledge of the composition and architecture of the extracellular matrix of biofilms of S. aureus, as well as evidence that the investigation of alternative products to assist in the control and combat of biofilms is a promising strategy.
31456169	1	34	theme	various	115:121	arg1	infections					131:140	various medical infections	115:140	various medical infections	115:140	Bacterial biofilms are involved in various medical infections and food contamination episodes and, for this reason, it is of great importance to developing new strategies of its prevention and control.
31456169	4	35	from	formation	727:735	arg1	presence					771:778	the presence	767:778	the presence of nisin	767:787	The main components (polysaccharides, proteins, and extracellular DNA (eDNA)) of the biofilm matrix were determined by spectrophotometry and showed that the formation of staphylococcal biofilms in the presence of nisin results in a less dense matrix structure with modification in its constituents.
31456169	5	36	dep	control	1094:1100	arg1	the					1090:1092	the	1090:1092	the	1090:1092	These results contribute to increase the knowledge of the composition and architecture of the extracellular matrix of biofilms of S. aureus, as well as evidence that the investigation of alternative products to assist in the control and combat of biofilms is a promising strategy.
31456169	5	37	theme	architecture	943:954	arg1	knowledge					910:918	the knowledge	906:918	the knowledge of the composition and architecture of the extracellular matrix of biofilms of S. aureus	906:1007	These results contribute to increase the knowledge of the composition and architecture of the extracellular matrix of biofilms of S. aureus, as well as evidence that the investigation of alternative products to assist in the control and combat of biofilms is a promising strategy.
31456169	0	38	dep	composition	15:25	arg1	the					11:13	the	11:13	the	11:13	Changes in the composition and architecture of staphylococcal biofilm by nisin.
31456169	1	39	theme	medical	123:129	arg1	infections					131:140	various medical infections	115:140	various medical infections	115:140	Bacterial biofilms are involved in various medical infections and food contamination episodes and, for this reason, it is of great importance to developing new strategies of its prevention and control.
31456169	4	40	theme	extracellular	622:634	arg1	eDNA					641:644	eDNA	641:644	eDNA	641:644	The main components (polysaccharides, proteins, and extracellular DNA (eDNA)) of the biofilm matrix were determined by spectrophotometry and showed that the formation of staphylococcal biofilms in the presence of nisin results in a less dense matrix structure with modification in its constituents.
31456169	4	40	theme	extracellular	622:634	arg1	DNA					636:638	extracellular DNA	622:638	extracellular DNA (eDNA)	622:645	The main components (polysaccharides, proteins, and extracellular DNA (eDNA)) of the biofilm matrix were determined by spectrophotometry and showed that the formation of staphylococcal biofilms in the presence of nisin results in a less dense matrix structure with modification in its constituents.
31456169	5	41	theme	matrix	977:982	arg1	architecture					943:954	architecture	943:954	architecture	943:954	These results contribute to increase the knowledge of the composition and architecture of the extracellular matrix of biofilms of S. aureus, as well as evidence that the investigation of alternative products to assist in the control and combat of biofilms is a promising strategy.
31456169	5	41	theme	matrix	977:982	arg1	composition					927:937	composition	927:937	composition	927:937	These results contribute to increase the knowledge of the composition and architecture of the extracellular matrix of biofilms of S. aureus, as well as evidence that the investigation of alternative products to assist in the control and combat of biofilms is a promising strategy.
31456169	3	42	theme	morphological	498:510	arg1	changes					512:518	morphological changes	498:518	morphological changes in the architecture of the structure of biofilms	498:567	Results obtained by confocal laser microscopy demonstrated morphological changes in the architecture of the structure of biofilms.
31456169	1	43	theme	great	205:209	arg1	importance					211:220	great importance	205:220	great importance	205:220	Bacterial biofilms are involved in various medical infections and food contamination episodes and, for this reason, it is of great importance to developing new strategies of its prevention and control.
31456169	5	44	theme	biofilms	987:994	arg1	matrix					977:982	the extracellular matrix	959:982	the extracellular matrix of biofilms of S. aureus	959:1007	These results contribute to increase the knowledge of the composition and architecture of the extracellular matrix of biofilms of S. aureus, as well as evidence that the investigation of alternative products to assist in the control and combat of biofilms is a promising strategy.
31456169	5	45	theme	extracellular	963:975	arg1	matrix					977:982	the extracellular matrix	959:982	the extracellular matrix of biofilms of S. aureus	959:1007	These results contribute to increase the knowledge of the composition and architecture of the extracellular matrix of biofilms of S. aureus, as well as evidence that the investigation of alternative products to assist in the control and combat of biofilms is a promising strategy.
31456169	0	46	theme	biofilm	62:68	arg1	architecture					31:42	architecture	31:42	architecture	31:42	Changes in the composition and architecture of staphylococcal biofilm by nisin.
31456169	0	46	theme	biofilm	62:68	arg1	composition					15:25	composition	15:25	composition	15:25	Changes in the composition and architecture of staphylococcal biofilm by nisin.
31456169	1	47	theme	food	146:149	arg1	episodes					165:172	food contamination episodes	146:172	food contamination episodes	146:172	Bacterial biofilms are involved in various medical infections and food contamination episodes and, for this reason, it is of great importance to developing new strategies of its prevention and control.
30383855	4	0	theme	Proteobacteria	1082:1095	arg1	phyla					1097:1101	Proteobacteria phyla	1082:1101	Proteobacteria phyla	1082:1101	While mucin-degrading bacterium, Akkermansia muciniphila (A. muciniphila) along with Enterobacteriaceae belonging to Proteobacteria phyla increased in the feces, antimicrobial peptides Angiogenin-4, Intelectin-1 and Intelectin-2, and ISC marker Dclk1, exhibited dramatic decreases in the colons of CR-infected/DBZ-treated mice.
30383855	6	1	theme	injury	1527:1532	arg1	exacerbation					1503:1514	exacerbation	1503:1514	exacerbation of mucosal injury	1503:1532	These changes coincided with the loss of Notch signaling and exacerbation of mucosal injury.
30383855	6	1	theme	injury	1527:1532	arg1	loss					1475:1478	the loss	1471:1478	the loss of Notch signaling	1471:1497	These changes coincided with the loss of Notch signaling and exacerbation of mucosal injury.
30383855	3	2	dep	DBZ	670:672	arg1	b.w.					691:694	10μmol/Kg b.w.	681:694	10μmol/Kg b.w.	681:694	In the current study, mucus analysis in the colons of CR-infected (108 CFUs) and Notch blocker Dibenzazepine (DBZ, i.p.; 10μmol/Kg b.w.)-treated mice revealed significant alterations in the composition of trace O-glycans and complex type and hybrid N-glycans, compared to CR-infected mice alone that preceded/accompanied alterations in 16S rDNA microbial community structure and elevated EUB338 staining.
30383855	9	3	theme	antibiotics	1950:1960	arg1	use					1943:1945	Controlled use	1932:1945	Controlled use of antibiotics	1932:1960	Controlled use of antibiotics seems to alleviate gut dysbiosis but may be insufficient to promote colonic crypt regeneration.
30383855	3	4	theme	-treated	696:703	arg1	mice					705:708	CR-infected (108 CFUs) and Notch blocker Dibenzazepine (DBZ, i.p.; 10μmol/Kg b.w.)-treated mice	614:708	CR-infected (108 CFUs) and Notch blocker Dibenzazepine (DBZ, i.p.; 10μmol/Kg b.w.)-treated mice	614:708	In the current study, mucus analysis in the colons of CR-infected (108 CFUs) and Notch blocker Dibenzazepine (DBZ, i.p.; 10μmol/Kg b.w.)-treated mice revealed significant alterations in the composition of trace O-glycans and complex type and hybrid N-glycans, compared to CR-infected mice alone that preceded/accompanied alterations in 16S rDNA microbial community structure and elevated EUB338 staining.
30383855	3	5	dep	CR-infected	614:624	arg1	CFUs					631:634	108 CFUs	627:634	108 CFUs	627:634	In the current study, mucus analysis in the colons of CR-infected (108 CFUs) and Notch blocker Dibenzazepine (DBZ, i.p.; 10μmol/Kg b.w.)-treated mice revealed significant alterations in the composition of trace O-glycans and complex type and hybrid N-glycans, compared to CR-infected mice alone that preceded/accompanied alterations in 16S rDNA microbial community structure and elevated EUB338 staining.
30383855	7	6	from	permeability	1778:1789	arg1	crypt					1741:1745	the crypt	1737:1745	the crypt	1737:1745	In response to a cocktail of antibiotics (Metronidazole/ciprofloxacin) for 10 days, there was increased survival that coincided with: i) decreased levels of Proteobacteria, ii) elevated Dclk1 levels in the crypt and, iii) reduced paracellular permeability.
30383855	9	7	theme	gut	1981:1983	arg1	dysbiosis					1985:1993	gut dysbiosis	1981:1993	gut dysbiosis	1981:1993	Controlled use of antibiotics seems to alleviate gut dysbiosis but may be insufficient to promote colonic crypt regeneration.
30383855	0	8	theme	barrier	119:125	arg1	disruption					127:136	barrier disruption	119:136	barrier disruption	119:136	Enteric infection coupled with chronic Notch pathway inhibition alters colonic mucus composition leading to dysbiosis, barrier disruption and colitis.
30383855	3	9	theme	mucus	582:586	arg1	analysis					588:595	mucus analysis	582:595	mucus analysis in the colons of CR-infected (108 CFUs) and Notch blocker Dibenzazepine (DBZ, i.p.; 10μmol/Kg b.w.)-treated mice	582:708	In the current study, mucus analysis in the colons of CR-infected (108 CFUs) and Notch blocker Dibenzazepine (DBZ, i.p.; 10μmol/Kg b.w.)-treated mice revealed significant alterations in the composition of trace O-glycans and complex type and hybrid N-glycans, compared to CR-infected mice alone that preceded/accompanied alterations in 16S rDNA microbial community structure and elevated EUB338 staining.
30383855	7	10	theme	elevated	1712:1719	arg1	levels					1727:1732	elevated Dclk1 levels	1712:1732	ii) elevated Dclk1 levels in the crypt	1708:1745	In response to a cocktail of antibiotics (Metronidazole/ciprofloxacin) for 10 days, there was increased survival that coincided with: i) decreased levels of Proteobacteria, ii) elevated Dclk1 levels in the crypt and, iii) reduced paracellular permeability.
30383855	3	11	theme	O-glycans	771:779	arg1	composition					750:760	the composition	746:760	the composition of trace O-glycans and complex type and hybrid N-glycans	746:817	In the current study, mucus analysis in the colons of CR-infected (108 CFUs) and Notch blocker Dibenzazepine (DBZ, i.p.; 10μmol/Kg b.w.)-treated mice revealed significant alterations in the composition of trace O-glycans and complex type and hybrid N-glycans, compared to CR-infected mice alone that preceded/accompanied alterations in 16S rDNA microbial community structure and elevated EUB338 staining.
30383855	3	12	theme	CR-infected	614:624	arg1	mice					705:708	CR-infected (108 CFUs) and Notch blocker Dibenzazepine (DBZ, i.p.; 10μmol/Kg b.w.)-treated mice	614:708	CR-infected (108 CFUs) and Notch blocker Dibenzazepine (DBZ, i.p.; 10μmol/Kg b.w.)-treated mice	614:708	In the current study, mucus analysis in the colons of CR-infected (108 CFUs) and Notch blocker Dibenzazepine (DBZ, i.p.; 10μmol/Kg b.w.)-treated mice revealed significant alterations in the composition of trace O-glycans and complex type and hybrid N-glycans, compared to CR-infected mice alone that preceded/accompanied alterations in 16S rDNA microbial community structure and elevated EUB338 staining.
30383855	3	13	dep	rDNA	900:903	arg1	structure					925:933	microbial community structure	905:933	microbial community structure	905:933	In the current study, mucus analysis in the colons of CR-infected (108 CFUs) and Notch blocker Dibenzazepine (DBZ, i.p.; 10μmol/Kg b.w.)-treated mice revealed significant alterations in the composition of trace O-glycans and complex type and hybrid N-glycans, compared to CR-infected mice alone that preceded/accompanied alterations in 16S rDNA microbial community structure and elevated EUB338 staining.
30383855	7	14	theme	decreased	1672:1680	arg1	levels					1682:1687	decreased levels	1672:1687	: i) decreased levels of Proteobacteria	1667:1705	In response to a cocktail of antibiotics (Metronidazole/ciprofloxacin) for 10 days, there was increased survival that coincided with: i) decreased levels of Proteobacteria, ii) elevated Dclk1 levels in the crypt and, iii) reduced paracellular permeability.
30383855	4	15	dep	muciniphila	1010:1020	arg1	muciniphila					1026:1036	A. muciniphila	1023:1036	A. muciniphila	1023:1036	While mucin-degrading bacterium, Akkermansia muciniphila (A. muciniphila) along with Enterobacteriaceae belonging to Proteobacteria phyla increased in the feces, antimicrobial peptides Angiogenin-4, Intelectin-1 and Intelectin-2, and ISC marker Dclk1, exhibited dramatic decreases in the colons of CR-infected/DBZ-treated mice.
30383855	1	16	from	modification	204:215	arg1	conjunction					220:230	conjunction	220:230	conjunction with tight junction (TJ) changes	220:263	Intestinal mucus layer disruption and gut microflora modification in conjunction with tight junction (TJ) changes can increase colonic permeability that allows bacterial dissemination and intestinal and systemic disease.
30383855	7	17	from	levels	1727:1732	arg1	crypt					1741:1745	the crypt	1737:1745	the crypt	1737:1745	In response to a cocktail of antibiotics (Metronidazole/ciprofloxacin) for 10 days, there was increased survival that coincided with: i) decreased levels of Proteobacteria, ii) elevated Dclk1 levels in the crypt and, iii) reduced paracellular permeability.
30383855	8	18	theme	enteric	1798:1804	arg1	infections					1806:1815	enteric infections	1798:1815	enteric infections that interfere with Notch activity	1798:1850	Thus, enteric infections that interfere with Notch activity may promote mucosal dysbiosis that is preceded by changes in mucus composition.
30383855	7	19	theme	reduced	1757:1763	arg1	permeability					1778:1789	reduced paracellular permeability	1757:1789	iii) reduced paracellular permeability	1752:1789	In response to a cocktail of antibiotics (Metronidazole/ciprofloxacin) for 10 days, there was increased survival that coincided with: i) decreased levels of Proteobacteria, ii) elevated Dclk1 levels in the crypt and, iii) reduced paracellular permeability.
30383855	4	20	theme	ISC	1199:1201	arg1	Dclk1					1210:1214	ISC marker Dclk1	1199:1214	ISC marker Dclk1	1199:1214	While mucin-degrading bacterium, Akkermansia muciniphila (A. muciniphila) along with Enterobacteriaceae belonging to Proteobacteria phyla increased in the feces, antimicrobial peptides Angiogenin-4, Intelectin-1 and Intelectin-2, and ISC marker Dclk1, exhibited dramatic decreases in the colons of CR-infected/DBZ-treated mice.
30383855	5	21	theme	junction	1336:1343	arg1	immuno-staining					1353:1367	adherens junction protein immuno-staining	1327:1367	adherens junction protein immuno-staining within the colonic crypts that negatively impacted paracellular barrier	1327:1439	Also evident was a loss of TJ and adherens junction protein immuno-staining within the colonic crypts that negatively impacted paracellular barrier.
30383855	3	22	theme	complex	785:791	arg1	type					793:796	complex type and hybrid N-glycans	785:817	type	793:796	In the current study, mucus analysis in the colons of CR-infected (108 CFUs) and Notch blocker Dibenzazepine (DBZ, i.p.; 10μmol/Kg b.w.)-treated mice revealed significant alterations in the composition of trace O-glycans and complex type and hybrid N-glycans, compared to CR-infected mice alone that preceded/accompanied alterations in 16S rDNA microbial community structure and elevated EUB338 staining.
30383855	7	23	dep	levels	1682:1687	arg1	i					1669:1669	i	1669:1669	i	1669:1669	In response to a cocktail of antibiotics (Metronidazole/ciprofloxacin) for 10 days, there was increased survival that coincided with: i) decreased levels of Proteobacteria, ii) elevated Dclk1 levels in the crypt and, iii) reduced paracellular permeability.
30383855	1	24	from	disruption	174:183	arg1	conjunction					220:230	conjunction	220:230	conjunction with tight junction (TJ) changes	220:263	Intestinal mucus layer disruption and gut microflora modification in conjunction with tight junction (TJ) changes can increase colonic permeability that allows bacterial dissemination and intestinal and systemic disease.
30383855	1	25	theme	Intestinal	151:160	arg1	disruption					174:183	Intestinal mucus layer disruption	151:183	Intestinal mucus layer disruption	151:183	Intestinal mucus layer disruption and gut microflora modification in conjunction with tight junction (TJ) changes can increase colonic permeability that allows bacterial dissemination and intestinal and systemic disease.
30383855	0	26	theme	mucus	79:83	arg1	composition					85:95	colonic mucus composition	71:95	colonic mucus composition leading to dysbiosis, barrier disruption and colitis	71:148	Enteric infection coupled with chronic Notch pathway inhibition alters colonic mucus composition leading to dysbiosis, barrier disruption and colitis.
30383855	1	27	theme	layer	168:172	arg1	disruption					174:183	Intestinal mucus layer disruption	151:183	Intestinal mucus layer disruption	151:183	Intestinal mucus layer disruption and gut microflora modification in conjunction with tight junction (TJ) changes can increase colonic permeability that allows bacterial dissemination and intestinal and systemic disease.
30383855	3	28	theme	preceded/accompanied	860:879	arg1	alterations					881:891	preceded/accompanied alterations	860:891	preceded/accompanied alterations in 16S rDNA microbial community structure and elevated EUB338 staining	860:962	In the current study, mucus analysis in the colons of CR-infected (108 CFUs) and Notch blocker Dibenzazepine (DBZ, i.p.; 10μmol/Kg b.w.)-treated mice revealed significant alterations in the composition of trace O-glycans and complex type and hybrid N-glycans, compared to CR-infected mice alone that preceded/accompanied alterations in 16S rDNA microbial community structure and elevated EUB338 staining.
30383855	7	29	theme	increased	1629:1637	arg1	survival					1639:1646	increased survival	1629:1646	increased survival that coincided with: i) decreased levels of Proteobacteria, ii) elevated Dclk1 levels in the crypt and, iii) reduced paracellular permeability	1629:1789	In response to a cocktail of antibiotics (Metronidazole/ciprofloxacin) for 10 days, there was increased survival that coincided with: i) decreased levels of Proteobacteria, ii) elevated Dclk1 levels in the crypt and, iii) reduced paracellular permeability.
30383855	4	30	theme	mice	1287:1290	arg1	colons					1253:1258	the colons	1249:1258	the colons of CR-infected/DBZ-treated mice	1249:1290	While mucin-degrading bacterium, Akkermansia muciniphila (A. muciniphila) along with Enterobacteriaceae belonging to Proteobacteria phyla increased in the feces, antimicrobial peptides Angiogenin-4, Intelectin-1 and Intelectin-2, and ISC marker Dclk1, exhibited dramatic decreases in the colons of CR-infected/DBZ-treated mice.
30383855	8	31	theme	mucosal	1864:1870	arg1	dysbiosis					1872:1880	mucosal dysbiosis	1864:1880	mucosal dysbiosis that is preceded by changes in mucus composition	1864:1929	Thus, enteric infections that interfere with Notch activity may promote mucosal dysbiosis that is preceded by changes in mucus composition.
30383855	3	32	theme	community	915:923	arg1	structure					925:933	microbial community structure	905:933	microbial community structure	905:933	In the current study, mucus analysis in the colons of CR-infected (108 CFUs) and Notch blocker Dibenzazepine (DBZ, i.p.; 10μmol/Kg b.w.)-treated mice revealed significant alterations in the composition of trace O-glycans and complex type and hybrid N-glycans, compared to CR-infected mice alone that preceded/accompanied alterations in 16S rDNA microbial community structure and elevated EUB338 staining.
30383855	2	33	theme	Wnt/β-catenin	536:548	arg1	pathways					550:557	Wnt/β-catenin pathways	536:557	Wnt/β-catenin pathways	536:557	We showed previously that Citrobacter rodentium (CR)-induced colonic crypt hyperplasia and/or colitis is regulated by a functional cross-talk between the Notch and Wnt/β-catenin pathways.
30383855	3	34	from	alterations	731:741	arg1	composition					750:760	the composition	746:760	the composition of trace O-glycans and complex type and hybrid N-glycans	746:817	In the current study, mucus analysis in the colons of CR-infected (108 CFUs) and Notch blocker Dibenzazepine (DBZ, i.p.; 10μmol/Kg b.w.)-treated mice revealed significant alterations in the composition of trace O-glycans and complex type and hybrid N-glycans, compared to CR-infected mice alone that preceded/accompanied alterations in 16S rDNA microbial community structure and elevated EUB338 staining.
30383855	3	35	theme	CR-infected	832:842	arg1	mice					844:847	CR-infected mice	832:847	CR-infected mice	832:847	In the current study, mucus analysis in the colons of CR-infected (108 CFUs) and Notch blocker Dibenzazepine (DBZ, i.p.; 10μmol/Kg b.w.)-treated mice revealed significant alterations in the composition of trace O-glycans and complex type and hybrid N-glycans, compared to CR-infected mice alone that preceded/accompanied alterations in 16S rDNA microbial community structure and elevated EUB338 staining.
30383855	0	36	theme	Notch	39:43	arg1	inhibition					53:62	chronic Notch pathway inhibition	31:62	chronic Notch pathway inhibition	31:62	Enteric infection coupled with chronic Notch pathway inhibition alters colonic mucus composition leading to dysbiosis, barrier disruption and colitis.
30383855	0	37	theme	Enteric	0:6	arg1	infection					8:16	Enteric infection	0:16	Enteric infection coupled with chronic Notch pathway inhibition	0:62	Enteric infection coupled with chronic Notch pathway inhibition alters colonic mucus composition leading to dysbiosis, barrier disruption and colitis.
30383855	1	38	theme	colonic	278:284	arg1	permeability					286:297	colonic permeability	278:297	colonic permeability that allows bacterial dissemination and intestinal and systemic disease	278:369	Intestinal mucus layer disruption and gut microflora modification in conjunction with tight junction (TJ) changes can increase colonic permeability that allows bacterial dissemination and intestinal and systemic disease.
30383855	3	39	theme	EUB338	948:953	arg1	staining					955:962	elevated EUB338 staining	939:962	elevated EUB338 staining	939:962	In the current study, mucus analysis in the colons of CR-infected (108 CFUs) and Notch blocker Dibenzazepine (DBZ, i.p.; 10μmol/Kg b.w.)-treated mice revealed significant alterations in the composition of trace O-glycans and complex type and hybrid N-glycans, compared to CR-infected mice alone that preceded/accompanied alterations in 16S rDNA microbial community structure and elevated EUB338 staining.
30383855	2	40	theme	crypt	441:445	arg1	hyperplasia					447:457	Citrobacter rodentium (CR)-induced colonic crypt hyperplasia	398:457	Citrobacter rodentium (CR)-induced colonic crypt hyperplasia	398:457	We showed previously that Citrobacter rodentium (CR)-induced colonic crypt hyperplasia and/or colitis is regulated by a functional cross-talk between the Notch and Wnt/β-catenin pathways.
30383855	6	41	theme	signaling	1489:1497	arg1	exacerbation					1503:1514	exacerbation	1503:1514	exacerbation of mucosal injury	1503:1532	These changes coincided with the loss of Notch signaling and exacerbation of mucosal injury.
30383855	6	41	theme	signaling	1489:1497	arg1	loss					1475:1478	the loss	1471:1478	the loss of Notch signaling	1471:1497	These changes coincided with the loss of Notch signaling and exacerbation of mucosal injury.
30383855	2	42	theme	functional	492:501	arg1	cross-talk					503:512	a functional cross-talk	490:512	a functional cross-talk between the Notch and Wnt/β-catenin pathways	490:557	We showed previously that Citrobacter rodentium (CR)-induced colonic crypt hyperplasia and/or colitis is regulated by a functional cross-talk between the Notch and Wnt/β-catenin pathways.
30383855	2	43	theme	-induced	424:431	arg1	hyperplasia					447:457	Citrobacter rodentium (CR)-induced colonic crypt hyperplasia	398:457	Citrobacter rodentium (CR)-induced colonic crypt hyperplasia	398:457	We showed previously that Citrobacter rodentium (CR)-induced colonic crypt hyperplasia and/or colitis is regulated by a functional cross-talk between the Notch and Wnt/β-catenin pathways.
30383855	0	44	theme	chronic	31:37	arg1	inhibition					53:62	chronic Notch pathway inhibition	31:62	chronic Notch pathway inhibition	31:62	Enteric infection coupled with chronic Notch pathway inhibition alters colonic mucus composition leading to dysbiosis, barrier disruption and colitis.
30383855	1	45	theme	bacterial	311:319	arg1	dissemination					321:333	bacterial dissemination	311:333	bacterial dissemination	311:333	Intestinal mucus layer disruption and gut microflora modification in conjunction with tight junction (TJ) changes can increase colonic permeability that allows bacterial dissemination and intestinal and systemic disease.
30383855	4	46	from	decreases	1236:1244	arg1	colons					1253:1258	the colons	1249:1258	the colons of CR-infected/DBZ-treated mice	1249:1290	While mucin-degrading bacterium, Akkermansia muciniphila (A. muciniphila) along with Enterobacteriaceae belonging to Proteobacteria phyla increased in the feces, antimicrobial peptides Angiogenin-4, Intelectin-1 and Intelectin-2, and ISC marker Dclk1, exhibited dramatic decreases in the colons of CR-infected/DBZ-treated mice.
30383855	3	47	theme	Notch	641:645	arg1	DBZ					670:672	DBZ	670:672	DBZ	670:672	In the current study, mucus analysis in the colons of CR-infected (108 CFUs) and Notch blocker Dibenzazepine (DBZ, i.p.; 10μmol/Kg b.w.)-treated mice revealed significant alterations in the composition of trace O-glycans and complex type and hybrid N-glycans, compared to CR-infected mice alone that preceded/accompanied alterations in 16S rDNA microbial community structure and elevated EUB338 staining.
30383855	3	47	theme	Notch	641:645	arg1	Dibenzazepine					655:667	Notch blocker Dibenzazepine	641:667	Notch blocker Dibenzazepine (DBZ, i.p.; 10μmol/Kg b.w.)	641:695	In the current study, mucus analysis in the colons of CR-infected (108 CFUs) and Notch blocker Dibenzazepine (DBZ, i.p.; 10μmol/Kg b.w.)-treated mice revealed significant alterations in the composition of trace O-glycans and complex type and hybrid N-glycans, compared to CR-infected mice alone that preceded/accompanied alterations in 16S rDNA microbial community structure and elevated EUB338 staining.
30383855	0	48	theme	pathway	45:51	arg1	inhibition					53:62	chronic Notch pathway inhibition	31:62	chronic Notch pathway inhibition	31:62	Enteric infection coupled with chronic Notch pathway inhibition alters colonic mucus composition leading to dysbiosis, barrier disruption and colitis.
30383855	8	49	theme	Notch	1837:1841	arg1	activity					1843:1850	Notch activity	1837:1850	Notch activity	1837:1850	Thus, enteric infections that interfere with Notch activity may promote mucosal dysbiosis that is preceded by changes in mucus composition.
30383855	1	50	theme	tight	237:241	arg1	TJ					253:254	TJ	253:254	TJ	253:254	Intestinal mucus layer disruption and gut microflora modification in conjunction with tight junction (TJ) changes can increase colonic permeability that allows bacterial dissemination and intestinal and systemic disease.
30383855	1	50	theme	tight	237:241	arg1	junction					243:250	tight junction	237:250	tight junction (TJ) changes	237:263	Intestinal mucus layer disruption and gut microflora modification in conjunction with tight junction (TJ) changes can increase colonic permeability that allows bacterial dissemination and intestinal and systemic disease.
30383855	4	51	theme	antimicrobial	1127:1139	arg1	Intelectin-2					1181:1192	Intelectin-2	1181:1192	Intelectin-2	1181:1192	While mucin-degrading bacterium, Akkermansia muciniphila (A. muciniphila) along with Enterobacteriaceae belonging to Proteobacteria phyla increased in the feces, antimicrobial peptides Angiogenin-4, Intelectin-1 and Intelectin-2, and ISC marker Dclk1, exhibited dramatic decreases in the colons of CR-infected/DBZ-treated mice.
30383855	4	51	theme	antimicrobial	1127:1139	arg1	peptides					1141:1148	antimicrobial peptides	1127:1148	antimicrobial peptides Angiogenin-4, Intelectin-1 and Intelectin-2	1127:1192	While mucin-degrading bacterium, Akkermansia muciniphila (A. muciniphila) along with Enterobacteriaceae belonging to Proteobacteria phyla increased in the feces, antimicrobial peptides Angiogenin-4, Intelectin-1 and Intelectin-2, and ISC marker Dclk1, exhibited dramatic decreases in the colons of CR-infected/DBZ-treated mice.
30383855	4	51	theme	antimicrobial	1127:1139	arg1	Intelectin-1					1164:1175	Intelectin-1	1164:1175	Intelectin-1	1164:1175	While mucin-degrading bacterium, Akkermansia muciniphila (A. muciniphila) along with Enterobacteriaceae belonging to Proteobacteria phyla increased in the feces, antimicrobial peptides Angiogenin-4, Intelectin-1 and Intelectin-2, and ISC marker Dclk1, exhibited dramatic decreases in the colons of CR-infected/DBZ-treated mice.
30383855	4	51	theme	antimicrobial	1127:1139	arg1	Angiogenin-4					1150:1161	Angiogenin-4	1150:1161	Angiogenin-4	1150:1161	While mucin-degrading bacterium, Akkermansia muciniphila (A. muciniphila) along with Enterobacteriaceae belonging to Proteobacteria phyla increased in the feces, antimicrobial peptides Angiogenin-4, Intelectin-1 and Intelectin-2, and ISC marker Dclk1, exhibited dramatic decreases in the colons of CR-infected/DBZ-treated mice.
30383855	5	52	theme	paracellular	1420:1431	arg1	barrier					1433:1439	paracellular barrier	1420:1439	paracellular barrier	1420:1439	Also evident was a loss of TJ and adherens junction protein immuno-staining within the colonic crypts that negatively impacted paracellular barrier.
30383855	9	53	theme	colonic	2030:2036	arg1	regeneration					2044:2055	colonic crypt regeneration	2030:2055	colonic crypt regeneration	2030:2055	Controlled use of antibiotics seems to alleviate gut dysbiosis but may be insufficient to promote colonic crypt regeneration.
30383855	4	54	dep	along	1039:1043	arg1	with					1045:1048	with	1045:1048	with	1045:1048	While mucin-degrading bacterium, Akkermansia muciniphila (A. muciniphila) along with Enterobacteriaceae belonging to Proteobacteria phyla increased in the feces, antimicrobial peptides Angiogenin-4, Intelectin-1 and Intelectin-2, and ISC marker Dclk1, exhibited dramatic decreases in the colons of CR-infected/DBZ-treated mice.
30383855	9	55	theme	Controlled	1932:1941	arg1	use					1943:1945	Controlled use	1932:1945	Controlled use of antibiotics	1932:1960	Controlled use of antibiotics seems to alleviate gut dysbiosis but may be insufficient to promote colonic crypt regeneration.
30383855	3	56	theme	10μmol/Kg	681:689	arg1	b.w.					691:694	10μmol/Kg b.w.	681:694	10μmol/Kg b.w.	681:694	In the current study, mucus analysis in the colons of CR-infected (108 CFUs) and Notch blocker Dibenzazepine (DBZ, i.p.; 10μmol/Kg b.w.)-treated mice revealed significant alterations in the composition of trace O-glycans and complex type and hybrid N-glycans, compared to CR-infected mice alone that preceded/accompanied alterations in 16S rDNA microbial community structure and elevated EUB338 staining.
30383855	8	57	theme	mucus	1913:1917	arg1	composition					1919:1929	mucus composition	1913:1929	mucus composition	1913:1929	Thus, enteric infections that interfere with Notch activity may promote mucosal dysbiosis that is preceded by changes in mucus composition.
30383855	3	58	theme	current	567:573	arg1	study					575:579	the current study	563:579	the current study	563:579	In the current study, mucus analysis in the colons of CR-infected (108 CFUs) and Notch blocker Dibenzazepine (DBZ, i.p.; 10μmol/Kg b.w.)-treated mice revealed significant alterations in the composition of trace O-glycans and complex type and hybrid N-glycans, compared to CR-infected mice alone that preceded/accompanied alterations in 16S rDNA microbial community structure and elevated EUB338 staining.
30383855	6	59	theme	mucosal	1519:1525	arg1	injury					1527:1532	mucosal injury	1519:1532	mucosal injury	1519:1532	These changes coincided with the loss of Notch signaling and exacerbation of mucosal injury.
30383855	3	60	from	alterations	881:891	arg1	rDNA					900:903	16S rDNA microbial community structure and elevated EUB338 staining	896:962	rDNA	900:903	In the current study, mucus analysis in the colons of CR-infected (108 CFUs) and Notch blocker Dibenzazepine (DBZ, i.p.; 10μmol/Kg b.w.)-treated mice revealed significant alterations in the composition of trace O-glycans and complex type and hybrid N-glycans, compared to CR-infected mice alone that preceded/accompanied alterations in 16S rDNA microbial community structure and elevated EUB338 staining.
30383855	3	60	from	alterations	881:891	arg1	staining					955:962	elevated EUB338 staining	939:962	elevated EUB338 staining	939:962	In the current study, mucus analysis in the colons of CR-infected (108 CFUs) and Notch blocker Dibenzazepine (DBZ, i.p.; 10μmol/Kg b.w.)-treated mice revealed significant alterations in the composition of trace O-glycans and complex type and hybrid N-glycans, compared to CR-infected mice alone that preceded/accompanied alterations in 16S rDNA microbial community structure and elevated EUB338 staining.
30383855	3	61	theme	significant	719:729	arg1	alterations					731:741	significant alterations	719:741	significant alterations in the composition of trace O-glycans and complex type and hybrid N-glycans	719:817	In the current study, mucus analysis in the colons of CR-infected (108 CFUs) and Notch blocker Dibenzazepine (DBZ, i.p.; 10μmol/Kg b.w.)-treated mice revealed significant alterations in the composition of trace O-glycans and complex type and hybrid N-glycans, compared to CR-infected mice alone that preceded/accompanied alterations in 16S rDNA microbial community structure and elevated EUB338 staining.
30383855	5	62	dep	loss	1312:1315	arg1	evident					1298:1304	evident	1298:1304	evident	1298:1304	Also evident was a loss of TJ and adherens junction protein immuno-staining within the colonic crypts that negatively impacted paracellular barrier.
30383855	3	63	theme	mice	705:708	arg1	colons					604:609	the colons	600:609	the colons of CR-infected (108 CFUs) and Notch blocker Dibenzazepine (DBZ, i.p.; 10μmol/Kg b.w.)-treated mice	600:708	In the current study, mucus analysis in the colons of CR-infected (108 CFUs) and Notch blocker Dibenzazepine (DBZ, i.p.; 10μmol/Kg b.w.)-treated mice revealed significant alterations in the composition of trace O-glycans and complex type and hybrid N-glycans, compared to CR-infected mice alone that preceded/accompanied alterations in 16S rDNA microbial community structure and elevated EUB338 staining.
30383855	1	64	theme	gut	189:191	arg1	microflora					193:202	gut microflora	189:202	gut microflora modification	189:215	Intestinal mucus layer disruption and gut microflora modification in conjunction with tight junction (TJ) changes can increase colonic permeability that allows bacterial dissemination and intestinal and systemic disease.
30383855	7	65	theme	Proteobacteria	1692:1705	arg1	permeability					1778:1789	reduced paracellular permeability	1757:1789	iii) reduced paracellular permeability	1752:1789	In response to a cocktail of antibiotics (Metronidazole/ciprofloxacin) for 10 days, there was increased survival that coincided with: i) decreased levels of Proteobacteria, ii) elevated Dclk1 levels in the crypt and, iii) reduced paracellular permeability.
30383855	7	65	theme	Proteobacteria	1692:1705	arg1	levels					1727:1732	elevated Dclk1 levels	1712:1732	ii) elevated Dclk1 levels in the crypt	1708:1745	In response to a cocktail of antibiotics (Metronidazole/ciprofloxacin) for 10 days, there was increased survival that coincided with: i) decreased levels of Proteobacteria, ii) elevated Dclk1 levels in the crypt and, iii) reduced paracellular permeability.
30383855	7	65	theme	Proteobacteria	1692:1705	arg1	levels					1682:1687	decreased levels	1672:1687	: i) decreased levels of Proteobacteria	1667:1705	In response to a cocktail of antibiotics (Metronidazole/ciprofloxacin) for 10 days, there was increased survival that coincided with: i) decreased levels of Proteobacteria, ii) elevated Dclk1 levels in the crypt and, iii) reduced paracellular permeability.
30383855	1	66	theme	microflora	193:202	arg1	modification					204:215	gut microflora modification	189:215	gut microflora modification	189:215	Intestinal mucus layer disruption and gut microflora modification in conjunction with tight junction (TJ) changes can increase colonic permeability that allows bacterial dissemination and intestinal and systemic disease.
30383855	4	67	theme	dramatic	1227:1234	arg1	decreases					1236:1244	dramatic decreases	1227:1244	dramatic decreases in the colons of CR-infected/DBZ-treated mice	1227:1290	While mucin-degrading bacterium, Akkermansia muciniphila (A. muciniphila) along with Enterobacteriaceae belonging to Proteobacteria phyla increased in the feces, antimicrobial peptides Angiogenin-4, Intelectin-1 and Intelectin-2, and ISC marker Dclk1, exhibited dramatic decreases in the colons of CR-infected/DBZ-treated mice.
30383855	5	68	theme	TJ	1320:1321	arg1	loss					1312:1315	a loss	1310:1315	a loss of TJ	1310:1321	Also evident was a loss of TJ and adherens junction protein immuno-staining within the colonic crypts that negatively impacted paracellular barrier.
30383855	5	68	theme	TJ	1320:1321	arg1	immuno-staining					1353:1367	adherens junction protein immuno-staining	1327:1367	adherens junction protein immuno-staining within the colonic crypts that negatively impacted paracellular barrier	1327:1439	Also evident was a loss of TJ and adherens junction protein immuno-staining within the colonic crypts that negatively impacted paracellular barrier.
30383855	3	69	theme	trace	765:769	arg1	O-glycans					771:779	trace O-glycans	765:779	trace O-glycans	765:779	In the current study, mucus analysis in the colons of CR-infected (108 CFUs) and Notch blocker Dibenzazepine (DBZ, i.p.; 10μmol/Kg b.w.)-treated mice revealed significant alterations in the composition of trace O-glycans and complex type and hybrid N-glycans, compared to CR-infected mice alone that preceded/accompanied alterations in 16S rDNA microbial community structure and elevated EUB338 staining.
30383855	4	70	dep	peptides	1141:1148	arg1	Intelectin-2					1181:1192	Intelectin-2	1181:1192	Intelectin-2	1181:1192	While mucin-degrading bacterium, Akkermansia muciniphila (A. muciniphila) along with Enterobacteriaceae belonging to Proteobacteria phyla increased in the feces, antimicrobial peptides Angiogenin-4, Intelectin-1 and Intelectin-2, and ISC marker Dclk1, exhibited dramatic decreases in the colons of CR-infected/DBZ-treated mice.
30383855	4	70	dep	peptides	1141:1148	arg1	peptides					1141:1148	antimicrobial peptides	1127:1148	antimicrobial peptides Angiogenin-4, Intelectin-1 and Intelectin-2	1127:1192	While mucin-degrading bacterium, Akkermansia muciniphila (A. muciniphila) along with Enterobacteriaceae belonging to Proteobacteria phyla increased in the feces, antimicrobial peptides Angiogenin-4, Intelectin-1 and Intelectin-2, and ISC marker Dclk1, exhibited dramatic decreases in the colons of CR-infected/DBZ-treated mice.
30383855	4	70	dep	peptides	1141:1148	arg1	Intelectin-1					1164:1175	Intelectin-1	1164:1175	Intelectin-1	1164:1175	While mucin-degrading bacterium, Akkermansia muciniphila (A. muciniphila) along with Enterobacteriaceae belonging to Proteobacteria phyla increased in the feces, antimicrobial peptides Angiogenin-4, Intelectin-1 and Intelectin-2, and ISC marker Dclk1, exhibited dramatic decreases in the colons of CR-infected/DBZ-treated mice.
30383855	4	70	dep	peptides	1141:1148	arg1	Angiogenin-4					1150:1161	Angiogenin-4	1150:1161	Angiogenin-4	1150:1161	While mucin-degrading bacterium, Akkermansia muciniphila (A. muciniphila) along with Enterobacteriaceae belonging to Proteobacteria phyla increased in the feces, antimicrobial peptides Angiogenin-4, Intelectin-1 and Intelectin-2, and ISC marker Dclk1, exhibited dramatic decreases in the colons of CR-infected/DBZ-treated mice.
30383855	4	71	theme	marker	1203:1208	arg1	Dclk1					1210:1214	ISC marker Dclk1	1199:1214	ISC marker Dclk1	1199:1214	While mucin-degrading bacterium, Akkermansia muciniphila (A. muciniphila) along with Enterobacteriaceae belonging to Proteobacteria phyla increased in the feces, antimicrobial peptides Angiogenin-4, Intelectin-1 and Intelectin-2, and ISC marker Dclk1, exhibited dramatic decreases in the colons of CR-infected/DBZ-treated mice.
30383855	3	72	from	analysis	588:595	arg1	colons					604:609	the colons	600:609	the colons of CR-infected (108 CFUs) and Notch blocker Dibenzazepine (DBZ, i.p.; 10μmol/Kg b.w.)-treated mice	600:708	In the current study, mucus analysis in the colons of CR-infected (108 CFUs) and Notch blocker Dibenzazepine (DBZ, i.p.; 10μmol/Kg b.w.)-treated mice revealed significant alterations in the composition of trace O-glycans and complex type and hybrid N-glycans, compared to CR-infected mice alone that preceded/accompanied alterations in 16S rDNA microbial community structure and elevated EUB338 staining.
30383855	3	73	theme	hybrid	802:807	arg1	N-glycans					809:817	complex type and hybrid N-glycans	785:817	N-glycans	809:817	In the current study, mucus analysis in the colons of CR-infected (108 CFUs) and Notch blocker Dibenzazepine (DBZ, i.p.; 10μmol/Kg b.w.)-treated mice revealed significant alterations in the composition of trace O-glycans and complex type and hybrid N-glycans, compared to CR-infected mice alone that preceded/accompanied alterations in 16S rDNA microbial community structure and elevated EUB338 staining.
30383855	0	74	theme	colonic	71:77	arg1	composition					85:95	colonic mucus composition	71:95	colonic mucus composition leading to dysbiosis, barrier disruption and colitis	71:148	Enteric infection coupled with chronic Notch pathway inhibition alters colonic mucus composition leading to dysbiosis, barrier disruption and colitis.
30383855	7	75	theme	paracellular	1765:1776	arg1	permeability					1778:1789	reduced paracellular permeability	1757:1789	iii) reduced paracellular permeability	1752:1789	In response to a cocktail of antibiotics (Metronidazole/ciprofloxacin) for 10 days, there was increased survival that coincided with: i) decreased levels of Proteobacteria, ii) elevated Dclk1 levels in the crypt and, iii) reduced paracellular permeability.
30383855	9	76	theme	crypt	2038:2042	arg1	regeneration					2044:2055	colonic crypt regeneration	2030:2055	colonic crypt regeneration	2030:2055	Controlled use of antibiotics seems to alleviate gut dysbiosis but may be insufficient to promote colonic crypt regeneration.
30383855	3	77	theme	type	793:796	arg1	composition					750:760	the composition	746:760	the composition of trace O-glycans and complex type and hybrid N-glycans	746:817	In the current study, mucus analysis in the colons of CR-infected (108 CFUs) and Notch blocker Dibenzazepine (DBZ, i.p.; 10μmol/Kg b.w.)-treated mice revealed significant alterations in the composition of trace O-glycans and complex type and hybrid N-glycans, compared to CR-infected mice alone that preceded/accompanied alterations in 16S rDNA microbial community structure and elevated EUB338 staining.
30383855	1	78	theme	systemic	354:361	arg1	disease					363:369	intestinal and systemic disease	339:369	intestinal and systemic disease	339:369	Intestinal mucus layer disruption and gut microflora modification in conjunction with tight junction (TJ) changes can increase colonic permeability that allows bacterial dissemination and intestinal and systemic disease.
30383855	1	79	theme	mucus	162:166	arg1	disruption					174:183	Intestinal mucus layer disruption	151:183	Intestinal mucus layer disruption	151:183	Intestinal mucus layer disruption and gut microflora modification in conjunction with tight junction (TJ) changes can increase colonic permeability that allows bacterial dissemination and intestinal and systemic disease.
30383855	3	80	theme	16S	896:898	arg1	rDNA					900:903	16S rDNA microbial community structure and elevated EUB338 staining	896:962	rDNA	900:903	In the current study, mucus analysis in the colons of CR-infected (108 CFUs) and Notch blocker Dibenzazepine (DBZ, i.p.; 10μmol/Kg b.w.)-treated mice revealed significant alterations in the composition of trace O-glycans and complex type and hybrid N-glycans, compared to CR-infected mice alone that preceded/accompanied alterations in 16S rDNA microbial community structure and elevated EUB338 staining.
30383855	7	81	dep	permeability	1778:1789	arg1	iii					1752:1754	iii	1752:1754	iii	1752:1754	In response to a cocktail of antibiotics (Metronidazole/ciprofloxacin) for 10 days, there was increased survival that coincided with: i) decreased levels of Proteobacteria, ii) elevated Dclk1 levels in the crypt and, iii) reduced paracellular permeability.
30383855	5	82	theme	colonic	1380:1386	arg1	crypts					1388:1393	the colonic crypts	1376:1393	the colonic crypts that negatively impacted paracellular barrier	1376:1439	Also evident was a loss of TJ and adherens junction protein immuno-staining within the colonic crypts that negatively impacted paracellular barrier.
30383855	7	83	from	levels	1682:1687	arg1	crypt					1741:1745	the crypt	1737:1745	the crypt	1737:1745	In response to a cocktail of antibiotics (Metronidazole/ciprofloxacin) for 10 days, there was increased survival that coincided with: i) decreased levels of Proteobacteria, ii) elevated Dclk1 levels in the crypt and, iii) reduced paracellular permeability.
30383855	4	84	theme	mucin-degrading	971:985	arg1	bacterium					987:995	mucin-degrading bacterium	971:995	mucin-degrading bacterium	971:995	While mucin-degrading bacterium, Akkermansia muciniphila (A. muciniphila) along with Enterobacteriaceae belonging to Proteobacteria phyla increased in the feces, antimicrobial peptides Angiogenin-4, Intelectin-1 and Intelectin-2, and ISC marker Dclk1, exhibited dramatic decreases in the colons of CR-infected/DBZ-treated mice.
30383855	3	85	theme	microbial	905:913	arg1	structure					925:933	microbial community structure	905:933	microbial community structure	905:933	In the current study, mucus analysis in the colons of CR-infected (108 CFUs) and Notch blocker Dibenzazepine (DBZ, i.p.; 10μmol/Kg b.w.)-treated mice revealed significant alterations in the composition of trace O-glycans and complex type and hybrid N-glycans, compared to CR-infected mice alone that preceded/accompanied alterations in 16S rDNA microbial community structure and elevated EUB338 staining.
30383855	4	86	theme	CR-infected/DBZ-treated	1263:1285	arg1	mice					1287:1290	CR-infected/DBZ-treated mice	1263:1290	CR-infected/DBZ-treated mice	1263:1290	While mucin-degrading bacterium, Akkermansia muciniphila (A. muciniphila) along with Enterobacteriaceae belonging to Proteobacteria phyla increased in the feces, antimicrobial peptides Angiogenin-4, Intelectin-1 and Intelectin-2, and ISC marker Dclk1, exhibited dramatic decreases in the colons of CR-infected/DBZ-treated mice.
30383855	5	87	theme	protein	1345:1351	arg1	immuno-staining					1353:1367	adherens junction protein immuno-staining	1327:1367	adherens junction protein immuno-staining within the colonic crypts that negatively impacted paracellular barrier	1327:1439	Also evident was a loss of TJ and adherens junction protein immuno-staining within the colonic crypts that negatively impacted paracellular barrier.
30383855	2	88	theme	Citrobacter	398:408	arg1	CR					421:422	CR	421:422	CR	421:422	We showed previously that Citrobacter rodentium (CR)-induced colonic crypt hyperplasia and/or colitis is regulated by a functional cross-talk between the Notch and Wnt/β-catenin pathways.
30383855	2	88	theme	Citrobacter	398:408	arg1	rodentium					410:418	Citrobacter rodentium	398:418	Citrobacter rodentium (CR)	398:423	We showed previously that Citrobacter rodentium (CR)-induced colonic crypt hyperplasia and/or colitis is regulated by a functional cross-talk between the Notch and Wnt/β-catenin pathways.
30383855	1	89	with	conjunction	220:230	arg1	changes					257:263	tight junction (TJ) changes	237:263	tight junction (TJ) changes	237:263	Intestinal mucus layer disruption and gut microflora modification in conjunction with tight junction (TJ) changes can increase colonic permeability that allows bacterial dissemination and intestinal and systemic disease.
30383855	7	90	theme	Dclk1	1721:1725	arg1	levels					1727:1732	elevated Dclk1 levels	1712:1732	ii) elevated Dclk1 levels in the crypt	1708:1745	In response to a cocktail of antibiotics (Metronidazole/ciprofloxacin) for 10 days, there was increased survival that coincided with: i) decreased levels of Proteobacteria, ii) elevated Dclk1 levels in the crypt and, iii) reduced paracellular permeability.
30383855	8	91	from	changes	1902:1908	arg1	composition					1919:1929	mucus composition	1913:1929	mucus composition	1913:1929	Thus, enteric infections that interfere with Notch activity may promote mucosal dysbiosis that is preceded by changes in mucus composition.
30383855	3	92	theme	blocker	647:653	arg1	DBZ					670:672	DBZ	670:672	DBZ	670:672	In the current study, mucus analysis in the colons of CR-infected (108 CFUs) and Notch blocker Dibenzazepine (DBZ, i.p.; 10μmol/Kg b.w.)-treated mice revealed significant alterations in the composition of trace O-glycans and complex type and hybrid N-glycans, compared to CR-infected mice alone that preceded/accompanied alterations in 16S rDNA microbial community structure and elevated EUB338 staining.
30383855	3	92	theme	blocker	647:653	arg1	Dibenzazepine					655:667	Notch blocker Dibenzazepine	641:667	Notch blocker Dibenzazepine (DBZ, i.p.; 10μmol/Kg b.w.)	641:695	In the current study, mucus analysis in the colons of CR-infected (108 CFUs) and Notch blocker Dibenzazepine (DBZ, i.p.; 10μmol/Kg b.w.)-treated mice revealed significant alterations in the composition of trace O-glycans and complex type and hybrid N-glycans, compared to CR-infected mice alone that preceded/accompanied alterations in 16S rDNA microbial community structure and elevated EUB338 staining.
30383855	2	93	theme	colonic	433:439	arg1	hyperplasia					447:457	Citrobacter rodentium (CR)-induced colonic crypt hyperplasia	398:457	Citrobacter rodentium (CR)-induced colonic crypt hyperplasia	398:457	We showed previously that Citrobacter rodentium (CR)-induced colonic crypt hyperplasia and/or colitis is regulated by a functional cross-talk between the Notch and Wnt/β-catenin pathways.
30383855	6	94	theme	Notch	1483:1487	arg1	signaling					1489:1497	Notch signaling	1483:1497	Notch signaling	1483:1497	These changes coincided with the loss of Notch signaling and exacerbation of mucosal injury.
30383855	7	95	dep	cocktail	1552:1559	arg1	response					1538:1545	response	1538:1545	response	1538:1545	In response to a cocktail of antibiotics (Metronidazole/ciprofloxacin) for 10 days, there was increased survival that coincided with: i) decreased levels of Proteobacteria, ii) elevated Dclk1 levels in the crypt and, iii) reduced paracellular permeability.
30383855	3	96	theme	elevated	939:946	arg1	staining					955:962	elevated EUB338 staining	939:962	elevated EUB338 staining	939:962	In the current study, mucus analysis in the colons of CR-infected (108 CFUs) and Notch blocker Dibenzazepine (DBZ, i.p.; 10μmol/Kg b.w.)-treated mice revealed significant alterations in the composition of trace O-glycans and complex type and hybrid N-glycans, compared to CR-infected mice alone that preceded/accompanied alterations in 16S rDNA microbial community structure and elevated EUB338 staining.
30383855	1	97	theme	intestinal	339:348	arg1	disease					363:369	intestinal and systemic disease	339:369	intestinal and systemic disease	339:369	Intestinal mucus layer disruption and gut microflora modification in conjunction with tight junction (TJ) changes can increase colonic permeability that allows bacterial dissemination and intestinal and systemic disease.
30383855	7	98	dep	levels	1727:1732	arg1	ii					1708:1709	ii	1708:1709	ii	1708:1709	In response to a cocktail of antibiotics (Metronidazole/ciprofloxacin) for 10 days, there was increased survival that coincided with: i) decreased levels of Proteobacteria, ii) elevated Dclk1 levels in the crypt and, iii) reduced paracellular permeability.
30383855	1	99	theme	junction	243:250	arg1	changes					257:263	tight junction (TJ) changes	237:263	tight junction (TJ) changes	237:263	Intestinal mucus layer disruption and gut microflora modification in conjunction with tight junction (TJ) changes can increase colonic permeability that allows bacterial dissemination and intestinal and systemic disease.
30383855	7	100	theme	antibiotics	1564:1574	arg1	cocktail					1552:1559	a cocktail	1550:1559	a cocktail of antibiotics (Metronidazole/ciprofloxacin) for 10 days	1550:1616	In response to a cocktail of antibiotics (Metronidazole/ciprofloxacin) for 10 days, there was increased survival that coincided with: i) decreased levels of Proteobacteria, ii) elevated Dclk1 levels in the crypt and, iii) reduced paracellular permeability.
30383855	5	101	theme	adherens	1327:1334	arg1	immuno-staining					1353:1367	adherens junction protein immuno-staining	1327:1367	adherens junction protein immuno-staining within the colonic crypts that negatively impacted paracellular barrier	1327:1439	Also evident was a loss of TJ and adherens junction protein immuno-staining within the colonic crypts that negatively impacted paracellular barrier.
31629028	0	0	link	THP-1-derived	107:119	arg1	macrophages					121:131	THP-1-derived macrophages	107:131	THP-1-derived macrophages	107:131	Anti-inflammatory properties of Xylopia aethiopica leaves: Interference with pro-inflammatory cytokines in THP-1-derived macrophages and flavonoid profiling.
31629028	15	1	theme	aethiopica	2665:2674	arg1	effects					2651:2657	The anti-inflammatory effects	2629:2657	The anti-inflammatory effects of X. aethiopica	2629:2674	CONCLUSION The anti-inflammatory effects of X. aethiopica leaves are demonstrated experimentally, thus substantiating its use in folk Medicine.
31629028	9	2	from	Evaluation	1628:1637	arg1	macrophages					1679:1689	LPS-challenged THP-1 macrophages	1658:1689	LPS-challenged THP-1 macrophages	1658:1689	RESULTS Evaluation of TNF-α levels in LPS-challenged THP-1 macrophages evidenced a significant inhibition (>90%) upon treatment with the hydroethanolic extract obtained from X. aethiopica leaves at a concentration of 500 μg/mL.
31629028	6	3	theme	MATERIALS	1229:1237	arg1	Interference					1251:1262	MATERIALS AND METHODS Interference	1229:1262	MATERIALS AND METHODS Interference with cytokines	1229:1277	MATERIALS AND METHODS Interference with cytokines was evaluated by ELISA assay, through the quantification of TNF-α and IL-6 levels in the culture medium collected from LPS-activated THP-1-derived-macrophages.
31629028	9	4	with	treatment	1738:1746	arg1	extract					1772:1778	the hydroethanolic extract	1753:1778	the hydroethanolic extract obtained from X. aethiopica leaves at a concentration of 500 μg/mL	1753:1845	RESULTS Evaluation of TNF-α levels in LPS-challenged THP-1 macrophages evidenced a significant inhibition (>90%) upon treatment with the hydroethanolic extract obtained from X. aethiopica leaves at a concentration of 500 μg/mL.
31629028	2	5	theme	rheumatic	656:664	arg1	pain					666:669	rheumatic pain	656:669	rheumatic pain	656:669	As such, extracts obtained from a series of plants recorded in those surveys were screened for their anti-inflammatory properties, a hydroethanolic extract obtained from the leaves of Xylopia aethiopica (Dunal) A. Rich, (Annonaceae), used on the treatment of headache, muscular pain and rheumatic pain, scoring positively and being further investigated.
31629028	3	6	theme	STUDY	734:738	arg1	AIM					723:725	AIM	723:725	AIM OF THE STUDY	723:738	AIM OF THE STUDY In order to identify species with anti-inflammatory properties, extracts were screened for their ability to interfere with LPS-induced TNF-α levels.
31629028	10	7	theme	Additional	1848:1857	arg1	effects					1877:1883	Additional anti-inflammatory effects	1848:1883	Additional anti-inflammatory effects	1848:1883	Additional anti-inflammatory effects were recorded, including a significant decrease on IL-6 levels at 250 and 500 μg/mL.
31629028	7	8	theme	13-hydroperoxylinoleic	1524:1545	arg1	acid					1547:1550	13-hydroperoxylinoleic acid	1524:1550	13-hydroperoxylinoleic acid	1524:1550	Inhibition of 5-lipoxygenase was assessed based on the oxidation of linoleic acid to 13-hydroperoxylinoleic acid.
31629028	2	9	theme	pain	647:650	arg1	treatment					615:623	the treatment	611:623	the treatment of headache, muscular pain and rheumatic pain	611:669	As such, extracts obtained from a series of plants recorded in those surveys were screened for their anti-inflammatory properties, a hydroethanolic extract obtained from the leaves of Xylopia aethiopica (Dunal) A. Rich, (Annonaceae), used on the treatment of headache, muscular pain and rheumatic pain, scoring positively and being further investigated.
31629028	13	10	theme	main	2342:2345	arg1	kaempferol-3-O-rutinoside					2369:2393	kaempferol-3-O-rutinoside (8)	2369:2397	kaempferol-3-O-rutinoside (8)	2369:2397	The main phenolic constituent, kaempferol-3-O-rutinoside (8), was found to significantly contribute to the anti-inflammatory effects, namely through the inhibition of 5-LOX.
31629028	13	10	theme	main	2342:2345	arg1	constituent					2356:2366	The main phenolic constituent	2338:2366	The main phenolic constituent	2338:2366	The main phenolic constituent, kaempferol-3-O-rutinoside (8), was found to significantly contribute to the anti-inflammatory effects, namely through the inhibition of 5-LOX.
31629028	16	11	theme	significant	2959:2969	arg1	ability					2971:2977	a significant ability	2957:2977	a significant ability to interfere with conventional inflammatory targets	2957:3029	Relevantly, the observed anti-inflammatory properties can stimulate further studies in order to fully unveil the therapeutic potential of the plant, namely as a source of phenolic compounds with a significant ability to interfere with conventional inflammatory targets.
31629028	6	12	theme	ELISA	1296:1300	arg1	assay					1302:1306	ELISA assay	1296:1306	ELISA assay	1296:1306	MATERIALS AND METHODS Interference with cytokines was evaluated by ELISA assay, through the quantification of TNF-α and IL-6 levels in the culture medium collected from LPS-activated THP-1-derived-macrophages.
31629028	1	13	theme	several	255:261	arg1	species					263:269	several species	255:269	several species	255:269	ETHNOPHARMACOLOGICAL RELEVANCE Ethnopharmacological surveys on Guinea-Bissauan flora reveal that several species are used to treat or ameliorate the symptomatology of conditions with an inflammatory background.
31629028	4	14	theme	significant	895:905	arg1	effects					907:913	significant effects	895:913	significant effects	895:913	Since significant effects were recorded upon treatment with the extract of the leaves obtained from X. aethiopica, further assays were conducted to elucidate additional mechanisms underlying its anti-inflammatory potential.
31629028	12	15	gly	mono-O-glycosylated	2241:2259	arg1	flavonols					2261:2269	mono-O-glycosylated flavonols	2241:2269	mono-O-glycosylated flavonols (5-8)	2241:2275	Phenolic profiling allowed the identification and quantitation of eight constituents, including caffeoylquinic acids (1-3), mono-O-glycosylated flavonols (5-8), and the mono-O-glycosyl flavone luteolin-7-O-glucoside (4).
31629028	12	15	gly	mono-O-glycosylated	2241:2259	arg1	5-8					2272:2274	5-8	2272:2274	5-8	2272:2274	Phenolic profiling allowed the identification and quantitation of eight constituents, including caffeoylquinic acids (1-3), mono-O-glycosylated flavonols (5-8), and the mono-O-glycosyl flavone luteolin-7-O-glucoside (4).
31629028	7	16	theme	5-lipoxygenase	1453:1466	arg1	Inhibition					1439:1448	Inhibition	1439:1448	Inhibition of 5-lipoxygenase	1439:1466	Inhibition of 5-lipoxygenase was assessed based on the oxidation of linoleic acid to 13-hydroperoxylinoleic acid.
31629028	16	17	theme	conventional	2997:3008	arg1	targets					3023:3029	conventional inflammatory targets	2997:3029	conventional inflammatory targets	2997:3029	Relevantly, the observed anti-inflammatory properties can stimulate further studies in order to fully unveil the therapeutic potential of the plant, namely as a source of phenolic compounds with a significant ability to interfere with conventional inflammatory targets.
31629028	13	18	theme	phenolic	2347:2354	arg1	kaempferol-3-O-rutinoside					2369:2393	kaempferol-3-O-rutinoside (8)	2369:2397	kaempferol-3-O-rutinoside (8)	2369:2397	The main phenolic constituent, kaempferol-3-O-rutinoside (8), was found to significantly contribute to the anti-inflammatory effects, namely through the inhibition of 5-LOX.
31629028	13	18	theme	phenolic	2347:2354	arg1	constituent					2356:2366	The main phenolic constituent	2338:2366	The main phenolic constituent	2338:2366	The main phenolic constituent, kaempferol-3-O-rutinoside (8), was found to significantly contribute to the anti-inflammatory effects, namely through the inhibition of 5-LOX.
31629028	6	19	theme	levels	1354:1359	arg1	quantification					1321:1334	the quantification	1317:1334	the quantification of TNF-α and IL-6 levels in the culture medium collected from LPS-activated THP-1-derived-macrophages	1317:1436	MATERIALS AND METHODS Interference with cytokines was evaluated by ELISA assay, through the quantification of TNF-α and IL-6 levels in the culture medium collected from LPS-activated THP-1-derived-macrophages.
31629028	10	20	theme	significant	1912:1922	arg1	decrease					1924:1931	a significant decrease	1910:1931	a significant decrease on IL-6 levels at 250 and 500 μg/mL	1910:1967	Additional anti-inflammatory effects were recorded, including a significant decrease on IL-6 levels at 250 and 500 μg/mL.
31629028	9	21	from	macrophages	1679:1689	arg1	Evaluation					1628:1637	RESULTS Evaluation	1620:1637	RESULTS Evaluation of TNF-α levels in LPS-challenged THP-1 macrophages	1620:1689	RESULTS Evaluation of TNF-α levels in LPS-challenged THP-1 macrophages evidenced a significant inhibition (>90%) upon treatment with the hydroethanolic extract obtained from X. aethiopica leaves at a concentration of 500 μg/mL.
31629028	9	22	theme	500 μg/mL	1837:1845	arg1	concentration					1820:1832	a concentration	1818:1832	a concentration of 500 μg/mL	1818:1845	RESULTS Evaluation of TNF-α levels in LPS-challenged THP-1 macrophages evidenced a significant inhibition (>90%) upon treatment with the hydroethanolic extract obtained from X. aethiopica leaves at a concentration of 500 μg/mL.
31629028	14	23	from	effects	2524:2530	arg1	decrease					2539:2546	the decrease	2535:2546	the decrease of TNF-α and IL-6 levels caused by this phenolic compound	2535:2604	However, no effects on the decrease of TNF-α and IL-6 levels caused by this phenolic compound were found.
31629028	7	24	theme	linoleic	1507:1514	arg1	acid					1516:1519	linoleic acid	1507:1519	linoleic acid	1507:1519	Inhibition of 5-lipoxygenase was assessed based on the oxidation of linoleic acid to 13-hydroperoxylinoleic acid.
31629028	1	25	theme	RELEVANCE	179:187	arg1	surveys					210:216	ETHNOPHARMACOLOGICAL RELEVANCE Ethnopharmacological surveys	158:216	ETHNOPHARMACOLOGICAL RELEVANCE Ethnopharmacological surveys on Guinea-Bissauan flora	158:241	ETHNOPHARMACOLOGICAL RELEVANCE Ethnopharmacological surveys on Guinea-Bissauan flora reveal that several species are used to treat or ameliorate the symptomatology of conditions with an inflammatory background.
31629028	3	26	theme	TNF-α	875:879	arg1	levels					881:886	LPS-induced TNF-α levels	863:886	LPS-induced TNF-α levels	863:886	AIM OF THE STUDY In order to identify species with anti-inflammatory properties, extracts were screened for their ability to interfere with LPS-induced TNF-α levels.
31629028	4	27	theme	further	1004:1010	arg1	assays					1012:1017	further assays	1004:1017	further assays	1004:1017	Since significant effects were recorded upon treatment with the extract of the leaves obtained from X. aethiopica, further assays were conducted to elucidate additional mechanisms underlying its anti-inflammatory potential.
31629028	6	28	theme	METHODS	1243:1249	arg1	Interference					1251:1262	MATERIALS AND METHODS Interference	1229:1262	MATERIALS AND METHODS Interference with cytokines	1229:1277	MATERIALS AND METHODS Interference with cytokines was evaluated by ELISA assay, through the quantification of TNF-α and IL-6 levels in the culture medium collected from LPS-activated THP-1-derived-macrophages.
31629028	5	29	theme	plant	1170:1174	arg1	composition					1151:1161	the chemical composition	1138:1161	the chemical composition of the plant	1138:1174	Since little is known on the chemical composition of the plant, we also aimed to characterise its phenolic profile.
31629028	9	30	from	levels	1648:1653	arg1	macrophages					1679:1689	LPS-challenged THP-1 macrophages	1658:1689	LPS-challenged THP-1 macrophages	1658:1689	RESULTS Evaluation of TNF-α levels in LPS-challenged THP-1 macrophages evidenced a significant inhibition (>90%) upon treatment with the hydroethanolic extract obtained from X. aethiopica leaves at a concentration of 500 μg/mL.
31629028	8	31	theme	profile	1586:1592	arg1	Characterization					1553:1568	Characterization	1553:1568	Characterization of the phenolic profile	1553:1592	Characterization of the phenolic profile was attained by HPLC-DAD.
31629028	12	32	theme	constituents	2189:2200	arg1	quantitation					2167:2178	quantitation	2167:2178	quantitation	2167:2178	Phenolic profiling allowed the identification and quantitation of eight constituents, including caffeoylquinic acids (1-3), mono-O-glycosylated flavonols (5-8), and the mono-O-glycosyl flavone luteolin-7-O-glucoside (4).
31629028	12	32	theme	constituents	2189:2200	arg1	identification					2148:2161	identification	2148:2161	identification	2148:2161	Phenolic profiling allowed the identification and quantitation of eight constituents, including caffeoylquinic acids (1-3), mono-O-glycosylated flavonols (5-8), and the mono-O-glycosyl flavone luteolin-7-O-glucoside (4).
31629028	1	33	theme	Guinea-Bissauan	221:235	arg1	flora					237:241	Guinea-Bissauan flora	221:241	Guinea-Bissauan flora	221:241	ETHNOPHARMACOLOGICAL RELEVANCE Ethnopharmacological surveys on Guinea-Bissauan flora reveal that several species are used to treat or ameliorate the symptomatology of conditions with an inflammatory background.
31629028	0	34	theme	THP-1-derived	107:119	arg1	macrophages					121:131	THP-1-derived macrophages	107:131	THP-1-derived macrophages	107:131	Anti-inflammatory properties of Xylopia aethiopica leaves: Interference with pro-inflammatory cytokines in THP-1-derived macrophages and flavonoid profiling.
31629028	5	35	theme	chemical	1142:1149	arg1	composition					1151:1161	the chemical composition	1138:1161	the chemical composition of the plant	1138:1174	Since little is known on the chemical composition of the plant, we also aimed to characterise its phenolic profile.
31629028	2	36	dep	the	539:541	arg1	leaves					543:548	leaves	543:548	leaves	543:548	As such, extracts obtained from a series of plants recorded in those surveys were screened for their anti-inflammatory properties, a hydroethanolic extract obtained from the leaves of Xylopia aethiopica (Dunal) A. Rich, (Annonaceae), used on the treatment of headache, muscular pain and rheumatic pain, scoring positively and being further investigated.
31629028	15	37	theme	anti-inflammatory	2633:2649	arg1	effects					2651:2657	The anti-inflammatory effects	2629:2657	The anti-inflammatory effects of X. aethiopica	2629:2674	CONCLUSION The anti-inflammatory effects of X. aethiopica leaves are demonstrated experimentally, thus substantiating its use in folk Medicine.
31629028	9	38	theme	TNF-α	1642:1646	arg1	levels					1648:1653	TNF-α levels	1642:1653	TNF-α levels in LPS-challenged THP-1 macrophages	1642:1689	RESULTS Evaluation of TNF-α levels in LPS-challenged THP-1 macrophages evidenced a significant inhibition (>90%) upon treatment with the hydroethanolic extract obtained from X. aethiopica leaves at a concentration of 500 μg/mL.
31629028	10	39	from	decrease	1924:1931	arg1	levels					1941:1946	IL-6 levels	1936:1946	IL-6 levels at 250 and 500 μg/mL	1936:1967	Additional anti-inflammatory effects were recorded, including a significant decrease on IL-6 levels at 250 and 500 μg/mL.
31629028	6	40	with	Interference	1251:1262	arg1	cytokines					1269:1277	cytokines	1269:1277	cytokines	1269:1277	MATERIALS AND METHODS Interference with cytokines was evaluated by ELISA assay, through the quantification of TNF-α and IL-6 levels in the culture medium collected from LPS-activated THP-1-derived-macrophages.
31629028	2	41	theme	pain	666:669	arg1	treatment					615:623	the treatment	611:623	the treatment of headache, muscular pain and rheumatic pain	611:669	As such, extracts obtained from a series of plants recorded in those surveys were screened for their anti-inflammatory properties, a hydroethanolic extract obtained from the leaves of Xylopia aethiopica (Dunal) A. Rich, (Annonaceae), used on the treatment of headache, muscular pain and rheumatic pain, scoring positively and being further investigated.
31629028	0	42	theme	Anti-inflammatory	0:16	arg1	properties					18:27	Anti-inflammatory properties	0:27	Anti-inflammatory properties of Xylopia aethiopica	0:49	Anti-inflammatory properties of Xylopia aethiopica leaves: Interference with pro-inflammatory cytokines in THP-1-derived macrophages and flavonoid profiling.
31629028	5	43	theme	phenolic	1211:1218	arg1	profile					1220:1226	its phenolic profile	1207:1226	its phenolic profile	1207:1226	Since little is known on the chemical composition of the plant, we also aimed to characterise its phenolic profile.
31629028	9	44	theme	THP-1	1673:1677	arg1	macrophages					1679:1689	LPS-challenged THP-1 macrophages	1658:1689	LPS-challenged THP-1 macrophages	1658:1689	RESULTS Evaluation of TNF-α levels in LPS-challenged THP-1 macrophages evidenced a significant inhibition (>90%) upon treatment with the hydroethanolic extract obtained from X. aethiopica leaves at a concentration of 500 μg/mL.
31629028	4	45	theme	anti-inflammatory	1084:1100	arg1	potential					1102:1110	its anti-inflammatory potential	1080:1110	its anti-inflammatory potential	1080:1110	Since significant effects were recorded upon treatment with the extract of the leaves obtained from X. aethiopica, further assays were conducted to elucidate additional mechanisms underlying its anti-inflammatory potential.
31629028	2	46	dep	Dunal	573:577	arg1	A.					580:581	A.	580:581	A.	580:581	As such, extracts obtained from a series of plants recorded in those surveys were screened for their anti-inflammatory properties, a hydroethanolic extract obtained from the leaves of Xylopia aethiopica (Dunal) A. Rich, (Annonaceae), used on the treatment of headache, muscular pain and rheumatic pain, scoring positively and being further investigated.
31629028	4	47	with	treatment	934:942	arg1	extract					953:959	the extract	949:959	the extract of the leaves obtained from X. aethiopica	949:1001	Since significant effects were recorded upon treatment with the extract of the leaves obtained from X. aethiopica, further assays were conducted to elucidate additional mechanisms underlying its anti-inflammatory potential.
31629028	12	48	theme	flavone	2302:2308	arg1	4					2334:2334	4	2334:2334	4	2334:2334	Phenolic profiling allowed the identification and quantitation of eight constituents, including caffeoylquinic acids (1-3), mono-O-glycosylated flavonols (5-8), and the mono-O-glycosyl flavone luteolin-7-O-glucoside (4).
31629028	12	48	theme	flavone	2302:2308	arg1	luteolin-7-O-glucoside					2310:2331	the mono-O-glycosyl flavone luteolin-7-O-glucoside	2282:2331	the mono-O-glycosyl flavone luteolin-7-O-glucoside (4)	2282:2335	Phenolic profiling allowed the identification and quantitation of eight constituents, including caffeoylquinic acids (1-3), mono-O-glycosylated flavonols (5-8), and the mono-O-glycosyl flavone luteolin-7-O-glucoside (4).
31629028	0	49	theme	aethiopica	40:49	arg1	properties					18:27	Anti-inflammatory properties	0:27	Anti-inflammatory properties of Xylopia aethiopica	0:49	Anti-inflammatory properties of Xylopia aethiopica leaves: Interference with pro-inflammatory cytokines in THP-1-derived macrophages and flavonoid profiling.
31629028	9	50	dep	X.	1794:1795	arg1	aethiopica					1797:1806	X. aethiopica	1794:1806	X. aethiopica	1794:1806	RESULTS Evaluation of TNF-α levels in LPS-challenged THP-1 macrophages evidenced a significant inhibition (>90%) upon treatment with the hydroethanolic extract obtained from X. aethiopica leaves at a concentration of 500 μg/mL.
31629028	9	50	dep	X.	1794:1795	arg1	leaves					1808:1813	leaves	1808:1813	leaves at a concentration of 500 μg/mL	1808:1845	RESULTS Evaluation of TNF-α levels in LPS-challenged THP-1 macrophages evidenced a significant inhibition (>90%) upon treatment with the hydroethanolic extract obtained from X. aethiopica leaves at a concentration of 500 μg/mL.
31629028	9	51	theme	significant	1703:1713	arg1	inhibition					1715:1724	a significant inhibition	1701:1724	a significant inhibition (>90%)	1701:1731	RESULTS Evaluation of TNF-α levels in LPS-challenged THP-1 macrophages evidenced a significant inhibition (>90%) upon treatment with the hydroethanolic extract obtained from X. aethiopica leaves at a concentration of 500 μg/mL.
31629028	9	51	theme	significant	1703:1713	arg1	%					1730:1730	>90%	1727:1730	>90%	1727:1730	RESULTS Evaluation of TNF-α levels in LPS-challenged THP-1 macrophages evidenced a significant inhibition (>90%) upon treatment with the hydroethanolic extract obtained from X. aethiopica leaves at a concentration of 500 μg/mL.
31629028	1	52	with	conditions	325:334	arg1	background					357:366	an inflammatory background	341:366	an inflammatory background	341:366	ETHNOPHARMACOLOGICAL RELEVANCE Ethnopharmacological surveys on Guinea-Bissauan flora reveal that several species are used to treat or ameliorate the symptomatology of conditions with an inflammatory background.
31629028	16	53	theme	therapeutic	2875:2885	arg1	source					2923:2928	a source	2921:2928	a source of phenolic compounds with a significant ability to interfere with conventional inflammatory targets	2921:3029	Relevantly, the observed anti-inflammatory properties can stimulate further studies in order to fully unveil the therapeutic potential of the plant, namely as a source of phenolic compounds with a significant ability to interfere with conventional inflammatory targets.
31629028	16	53	theme	therapeutic	2875:2885	arg1	potential					2887:2895	the therapeutic potential	2871:2895	the therapeutic potential of the plant, namely	2871:2916	Relevantly, the observed anti-inflammatory properties can stimulate further studies in order to fully unveil the therapeutic potential of the plant, namely as a source of phenolic compounds with a significant ability to interfere with conventional inflammatory targets.
31629028	4	54	theme	additional	1047:1056	arg1	mechanisms					1058:1067	additional mechanisms	1047:1067	additional mechanisms underlying its anti-inflammatory potential	1047:1110	Since significant effects were recorded upon treatment with the extract of the leaves obtained from X. aethiopica, further assays were conducted to elucidate additional mechanisms underlying its anti-inflammatory potential.
31629028	14	55	theme	TNF-α	2551:2555	arg1	levels					2566:2571	TNF-α and IL-6 levels	2551:2571	TNF-α and IL-6 levels	2551:2571	However, no effects on the decrease of TNF-α and IL-6 levels caused by this phenolic compound were found.
31629028	3	56	dep	identify	752:759	arg1	to					749:750	to	749:750	to	749:750	AIM OF THE STUDY In order to identify species with anti-inflammatory properties, extracts were screened for their ability to interfere with LPS-induced TNF-α levels.
31629028	15	57	from	use	2740:2742	arg1	Medicine					2752:2759	folk Medicine	2747:2759	folk Medicine	2747:2759	CONCLUSION The anti-inflammatory effects of X. aethiopica leaves are demonstrated experimentally, thus substantiating its use in folk Medicine.
31629028	6	58	from	quantification	1321:1334	arg1	medium					1376:1381	the culture medium	1364:1381	the culture medium collected from LPS-activated THP-1-derived-macrophages	1364:1436	MATERIALS AND METHODS Interference with cytokines was evaluated by ELISA assay, through the quantification of TNF-α and IL-6 levels in the culture medium collected from LPS-activated THP-1-derived-macrophages.
31629028	16	59	theme	anti-inflammatory	2787:2803	arg1	properties					2805:2814	the observed anti-inflammatory properties	2774:2814	the observed anti-inflammatory properties	2774:2814	Relevantly, the observed anti-inflammatory properties can stimulate further studies in order to fully unveil the therapeutic potential of the plant, namely as a source of phenolic compounds with a significant ability to interfere with conventional inflammatory targets.
31629028	10	60	from	500 μg/mL	1959:1967	arg1	levels					1941:1946	IL-6 levels	1936:1946	IL-6 levels at 250 and 500 μg/mL	1936:1967	Additional anti-inflammatory effects were recorded, including a significant decrease on IL-6 levels at 250 and 500 μg/mL.
31629028	6	61	theme	TNF-α	1339:1343	arg1	levels					1354:1359	TNF-α and IL-6 levels	1339:1359	TNF-α and IL-6 levels in the culture medium collected from LPS-activated THP-1-derived-macrophages	1339:1436	MATERIALS AND METHODS Interference with cytokines was evaluated by ELISA assay, through the quantification of TNF-α and IL-6 levels in the culture medium collected from LPS-activated THP-1-derived-macrophages.
31629028	14	62	theme	IL-6	2561:2564	arg1	levels					2566:2571	TNF-α and IL-6 levels	2551:2571	TNF-α and IL-6 levels	2551:2571	However, no effects on the decrease of TNF-α and IL-6 levels caused by this phenolic compound were found.
31629028	2	63	theme	plants	413:418	arg1	series					403:408	a series	401:408	a series of plants recorded in those surveys	401:444	As such, extracts obtained from a series of plants recorded in those surveys were screened for their anti-inflammatory properties, a hydroethanolic extract obtained from the leaves of Xylopia aethiopica (Dunal) A. Rich, (Annonaceae), used on the treatment of headache, muscular pain and rheumatic pain, scoring positively and being further investigated.
31629028	14	64	theme	phenolic	2588:2595	arg1	compound					2597:2604	this phenolic compound	2583:2604	this phenolic compound	2583:2604	However, no effects on the decrease of TNF-α and IL-6 levels caused by this phenolic compound were found.
31629028	2	65	theme	headache	628:635	arg1	treatment					615:623	the treatment	611:623	the treatment of headache, muscular pain and rheumatic pain	611:669	As such, extracts obtained from a series of plants recorded in those surveys were screened for their anti-inflammatory properties, a hydroethanolic extract obtained from the leaves of Xylopia aethiopica (Dunal) A. Rich, (Annonaceae), used on the treatment of headache, muscular pain and rheumatic pain, scoring positively and being further investigated.
31629028	16	66	theme	further	2830:2836	arg1	studies					2838:2844	further studies	2830:2844	further studies	2830:2844	Relevantly, the observed anti-inflammatory properties can stimulate further studies in order to fully unveil the therapeutic potential of the plant, namely as a source of phenolic compounds with a significant ability to interfere with conventional inflammatory targets.
31629028	1	67	theme	conditions	325:334	arg1	symptomatology					307:320	the symptomatology	303:320	the symptomatology of conditions with an inflammatory background	303:366	ETHNOPHARMACOLOGICAL RELEVANCE Ethnopharmacological surveys on Guinea-Bissauan flora reveal that several species are used to treat or ameliorate the symptomatology of conditions with an inflammatory background.
31629028	9	68	theme	hydroethanolic	1757:1770	arg1	extract					1772:1778	the hydroethanolic extract	1753:1778	the hydroethanolic extract obtained from X. aethiopica leaves at a concentration of 500 μg/mL	1753:1845	RESULTS Evaluation of TNF-α levels in LPS-challenged THP-1 macrophages evidenced a significant inhibition (>90%) upon treatment with the hydroethanolic extract obtained from X. aethiopica leaves at a concentration of 500 μg/mL.
31629028	7	69	theme	acid	1516:1519	arg1	oxidation					1494:1502	the oxidation	1490:1502	the oxidation of linoleic acid to 13-hydroperoxylinoleic acid	1490:1550	Inhibition of 5-lipoxygenase was assessed based on the oxidation of linoleic acid to 13-hydroperoxylinoleic acid.
31629028	10	70	theme	anti-inflammatory	1859:1875	arg1	effects					1877:1883	Additional anti-inflammatory effects	1848:1883	Additional anti-inflammatory effects	1848:1883	Additional anti-inflammatory effects were recorded, including a significant decrease on IL-6 levels at 250 and 500 μg/mL.
31629028	13	71	theme	anti-inflammatory	2445:2461	arg1	effects					2463:2469	the anti-inflammatory effects	2441:2469	the anti-inflammatory effects	2441:2469	The main phenolic constituent, kaempferol-3-O-rutinoside (8), was found to significantly contribute to the anti-inflammatory effects, namely through the inhibition of 5-LOX.
31629028	12	72	theme	Phenolic	2117:2124	arg1	profiling					2126:2134	Phenolic profiling	2117:2134	Phenolic profiling	2117:2134	Phenolic profiling allowed the identification and quantitation of eight constituents, including caffeoylquinic acids (1-3), mono-O-glycosylated flavonols (5-8), and the mono-O-glycosyl flavone luteolin-7-O-glucoside (4).
31629028	14	73	theme	levels	2566:2571	arg1	decrease					2539:2546	the decrease	2535:2546	the decrease of TNF-α and IL-6 levels caused by this phenolic compound	2535:2604	However, no effects on the decrease of TNF-α and IL-6 levels caused by this phenolic compound were found.
31629028	16	74	theme	compounds	2942:2950	arg1	potential					2887:2895	the therapeutic potential	2871:2895	the therapeutic potential of the plant, namely	2871:2916	Relevantly, the observed anti-inflammatory properties can stimulate further studies in order to fully unveil the therapeutic potential of the plant, namely as a source of phenolic compounds with a significant ability to interfere with conventional inflammatory targets.
31629028	16	74	theme	compounds	2942:2950	arg1	source					2923:2928	a source	2921:2928	a source of phenolic compounds with a significant ability to interfere with conventional inflammatory targets	2921:3029	Relevantly, the observed anti-inflammatory properties can stimulate further studies in order to fully unveil the therapeutic potential of the plant, namely as a source of phenolic compounds with a significant ability to interfere with conventional inflammatory targets.
31629028	12	75	theme	mono-O-glycosylated	2241:2259	arg1	flavonols					2261:2269	mono-O-glycosylated flavonols	2241:2269	mono-O-glycosylated flavonols (5-8)	2241:2275	Phenolic profiling allowed the identification and quantitation of eight constituents, including caffeoylquinic acids (1-3), mono-O-glycosylated flavonols (5-8), and the mono-O-glycosyl flavone luteolin-7-O-glucoside (4).
31629028	12	75	theme	mono-O-glycosylated	2241:2259	arg1	5-8					2272:2274	5-8	2272:2274	5-8	2272:2274	Phenolic profiling allowed the identification and quantitation of eight constituents, including caffeoylquinic acids (1-3), mono-O-glycosylated flavonols (5-8), and the mono-O-glycosyl flavone luteolin-7-O-glucoside (4).
31629028	2	76	theme	muscular	638:645	arg1	pain					647:650	muscular pain	638:650	muscular pain	638:650	As such, extracts obtained from a series of plants recorded in those surveys were screened for their anti-inflammatory properties, a hydroethanolic extract obtained from the leaves of Xylopia aethiopica (Dunal) A. Rich, (Annonaceae), used on the treatment of headache, muscular pain and rheumatic pain, scoring positively and being further investigated.
31629028	11	77	from	concentrations	2054:2067	arg1	inhibition					2040:2049	significant inhibition	2028:2049	significant inhibition at concentrations ranging from 16 to 250 μg/mL (IC50 = 85 μg/mL)	2028:2114	The extract proved to be active towards 5-LOX, leading to significant inhibition at concentrations ranging from 16 to 250 μg/mL (IC50 = 85 μg/mL).
31629028	3	78	theme	anti-inflammatory	774:790	arg1	properties					792:801	anti-inflammatory properties	774:801	anti-inflammatory properties	774:801	AIM OF THE STUDY In order to identify species with anti-inflammatory properties, extracts were screened for their ability to interfere with LPS-induced TNF-α levels.
31629028	15	79	theme	folk	2747:2750	arg1	Medicine					2752:2759	folk Medicine	2747:2759	folk Medicine	2747:2759	CONCLUSION The anti-inflammatory effects of X. aethiopica leaves are demonstrated experimentally, thus substantiating its use in folk Medicine.
31629028	6	80	theme	culture	1368:1374	arg1	medium					1376:1381	the culture medium	1364:1381	the culture medium collected from LPS-activated THP-1-derived-macrophages	1364:1436	MATERIALS AND METHODS Interference with cytokines was evaluated by ELISA assay, through the quantification of TNF-α and IL-6 levels in the culture medium collected from LPS-activated THP-1-derived-macrophages.
31629028	10	81	theme	IL-6	1936:1939	arg1	levels					1941:1946	IL-6 levels	1936:1946	IL-6 levels at 250 and 500 μg/mL	1936:1967	Additional anti-inflammatory effects were recorded, including a significant decrease on IL-6 levels at 250 and 500 μg/mL.
31629028	4	82	theme	the	964:966	arg1	extract					953:959	the extract	949:959	the extract of the leaves obtained from X. aethiopica	949:1001	Since significant effects were recorded upon treatment with the extract of the leaves obtained from X. aethiopica, further assays were conducted to elucidate additional mechanisms underlying its anti-inflammatory potential.
31629028	1	83	used	used	275:278	arg2	species					263:269	several species	255:269	several species	255:269	ETHNOPHARMACOLOGICAL RELEVANCE Ethnopharmacological surveys on Guinea-Bissauan flora reveal that several species are used to treat or ameliorate the symptomatology of conditions with an inflammatory background.
31629028	16	84	theme	phenolic	2933:2940	arg1	compounds					2942:2950	phenolic compounds	2933:2950	phenolic compounds with a significant ability to interfere with conventional inflammatory targets	2933:3029	Relevantly, the observed anti-inflammatory properties can stimulate further studies in order to fully unveil the therapeutic potential of the plant, namely as a source of phenolic compounds with a significant ability to interfere with conventional inflammatory targets.
31629028	16	85	theme	plant	2904:2908	arg1	source					2923:2928	a source	2921:2928	a source of phenolic compounds with a significant ability to interfere with conventional inflammatory targets	2921:3029	Relevantly, the observed anti-inflammatory properties can stimulate further studies in order to fully unveil the therapeutic potential of the plant, namely as a source of phenolic compounds with a significant ability to interfere with conventional inflammatory targets.
31629028	16	85	theme	plant	2904:2908	arg1	potential					2887:2895	the therapeutic potential	2871:2895	the therapeutic potential of the plant, namely	2871:2916	Relevantly, the observed anti-inflammatory properties can stimulate further studies in order to fully unveil the therapeutic potential of the plant, namely as a source of phenolic compounds with a significant ability to interfere with conventional inflammatory targets.
31629028	6	86	theme	IL-6	1349:1352	arg1	levels					1354:1359	TNF-α and IL-6 levels	1339:1359	TNF-α and IL-6 levels in the culture medium collected from LPS-activated THP-1-derived-macrophages	1339:1436	MATERIALS AND METHODS Interference with cytokines was evaluated by ELISA assay, through the quantification of TNF-α and IL-6 levels in the culture medium collected from LPS-activated THP-1-derived-macrophages.
31629028	1	87	theme	ETHNOPHARMACOLOGICAL	158:177	arg1	surveys					210:216	ETHNOPHARMACOLOGICAL RELEVANCE Ethnopharmacological surveys	158:216	ETHNOPHARMACOLOGICAL RELEVANCE Ethnopharmacological surveys on Guinea-Bissauan flora	158:241	ETHNOPHARMACOLOGICAL RELEVANCE Ethnopharmacological surveys on Guinea-Bissauan flora reveal that several species are used to treat or ameliorate the symptomatology of conditions with an inflammatory background.
31629028	8	88	theme	phenolic	1577:1584	arg1	profile					1586:1592	the phenolic profile	1573:1592	the phenolic profile	1573:1592	Characterization of the phenolic profile was attained by HPLC-DAD.
31629028	12	89	theme	caffeoylquinic	2213:2226	arg1	1-3					2235:2237	1-3	2235:2237	1-3	2235:2237	Phenolic profiling allowed the identification and quantitation of eight constituents, including caffeoylquinic acids (1-3), mono-O-glycosylated flavonols (5-8), and the mono-O-glycosyl flavone luteolin-7-O-glucoside (4).
31629028	12	89	theme	caffeoylquinic	2213:2226	arg1	acids					2228:2232	caffeoylquinic acids	2213:2232	caffeoylquinic acids (1-3)	2213:2238	Phenolic profiling allowed the identification and quantitation of eight constituents, including caffeoylquinic acids (1-3), mono-O-glycosylated flavonols (5-8), and the mono-O-glycosyl flavone luteolin-7-O-glucoside (4).
31629028	16	90	with	compounds	2942:2950	arg1	ability					2971:2977	a significant ability	2957:2977	a significant ability to interfere with conventional inflammatory targets	2957:3029	Relevantly, the observed anti-inflammatory properties can stimulate further studies in order to fully unveil the therapeutic potential of the plant, namely as a source of phenolic compounds with a significant ability to interfere with conventional inflammatory targets.
31629028	0	91	theme	pro-inflammatory	77:92	arg1	cytokines					94:102	pro-inflammatory cytokines	77:102	pro-inflammatory cytokines	77:102	Anti-inflammatory properties of Xylopia aethiopica leaves: Interference with pro-inflammatory cytokines in THP-1-derived macrophages and flavonoid profiling.
31629028	1	92	theme	Ethnopharmacological	189:208	arg1	surveys					210:216	ETHNOPHARMACOLOGICAL RELEVANCE Ethnopharmacological surveys	158:216	ETHNOPHARMACOLOGICAL RELEVANCE Ethnopharmacological surveys on Guinea-Bissauan flora	158:241	ETHNOPHARMACOLOGICAL RELEVANCE Ethnopharmacological surveys on Guinea-Bissauan flora reveal that several species are used to treat or ameliorate the symptomatology of conditions with an inflammatory background.
31629028	4	93	dep	the	964:966	arg1	leaves					968:973	leaves	968:973	leaves	968:973	Since significant effects were recorded upon treatment with the extract of the leaves obtained from X. aethiopica, further assays were conducted to elucidate additional mechanisms underlying its anti-inflammatory potential.
31629028	9	94	theme	RESULTS	1620:1626	arg1	Evaluation					1628:1637	RESULTS Evaluation	1620:1637	RESULTS Evaluation of TNF-α levels in LPS-challenged THP-1 macrophages	1620:1689	RESULTS Evaluation of TNF-α levels in LPS-challenged THP-1 macrophages evidenced a significant inhibition (>90%) upon treatment with the hydroethanolic extract obtained from X. aethiopica leaves at a concentration of 500 μg/mL.
31629028	15	95	dep	CONCLUSION	2618:2627	arg1	leaves					2676:2681	leaves	2676:2681	leaves	2676:2681	CONCLUSION The anti-inflammatory effects of X. aethiopica leaves are demonstrated experimentally, thus substantiating its use in folk Medicine.
31629028	3	96	theme	LPS-induced	863:873	arg1	levels					881:886	LPS-induced TNF-α levels	863:886	LPS-induced TNF-α levels	863:886	AIM OF THE STUDY In order to identify species with anti-inflammatory properties, extracts were screened for their ability to interfere with LPS-induced TNF-α levels.
31629028	15	97	theme	X.	2662:2663	arg1	aethiopica					2665:2674	X. aethiopica	2662:2674	X. aethiopica	2662:2674	CONCLUSION The anti-inflammatory effects of X. aethiopica leaves are demonstrated experimentally, thus substantiating its use in folk Medicine.
31629028	0	98	from	Interference	59:70	arg1	macrophages					121:131	THP-1-derived macrophages	107:131	THP-1-derived macrophages	107:131	Anti-inflammatory properties of Xylopia aethiopica leaves: Interference with pro-inflammatory cytokines in THP-1-derived macrophages and flavonoid profiling.
31629028	0	98	from	Interference	59:70	arg1	profiling					147:155	flavonoid profiling	137:155	flavonoid profiling	137:155	Anti-inflammatory properties of Xylopia aethiopica leaves: Interference with pro-inflammatory cytokines in THP-1-derived macrophages and flavonoid profiling.
31629028	0	99	theme	flavonoid	137:145	arg1	profiling					147:155	flavonoid profiling	137:155	flavonoid profiling	137:155	Anti-inflammatory properties of Xylopia aethiopica leaves: Interference with pro-inflammatory cytokines in THP-1-derived macrophages and flavonoid profiling.
31629028	1	100	from	surveys	210:216	arg1	flora					237:241	Guinea-Bissauan flora	221:241	Guinea-Bissauan flora	221:241	ETHNOPHARMACOLOGICAL RELEVANCE Ethnopharmacological surveys on Guinea-Bissauan flora reveal that several species are used to treat or ameliorate the symptomatology of conditions with an inflammatory background.
31629028	9	101	theme	levels	1648:1653	arg1	Evaluation					1628:1637	RESULTS Evaluation	1620:1637	RESULTS Evaluation of TNF-α levels in LPS-challenged THP-1 macrophages	1620:1689	RESULTS Evaluation of TNF-α levels in LPS-challenged THP-1 macrophages evidenced a significant inhibition (>90%) upon treatment with the hydroethanolic extract obtained from X. aethiopica leaves at a concentration of 500 μg/mL.
31629028	4	102	theme	X.	989:990	arg1	aethiopica					992:1001	X. aethiopica	989:1001	X. aethiopica	989:1001	Since significant effects were recorded upon treatment with the extract of the leaves obtained from X. aethiopica, further assays were conducted to elucidate additional mechanisms underlying its anti-inflammatory potential.
31629028	6	103	theme	LPS-activated	1398:1410	arg1	THP-1-derived-macrophages					1412:1436	LPS-activated THP-1-derived-macrophages	1398:1436	LPS-activated THP-1-derived-macrophages	1398:1436	MATERIALS AND METHODS Interference with cytokines was evaluated by ELISA assay, through the quantification of TNF-α and IL-6 levels in the culture medium collected from LPS-activated THP-1-derived-macrophages.
31629028	3	104	with	species	761:767	arg1	properties					792:801	anti-inflammatory properties	774:801	anti-inflammatory properties	774:801	AIM OF THE STUDY In order to identify species with anti-inflammatory properties, extracts were screened for their ability to interfere with LPS-induced TNF-α levels.
31629028	9	105	theme	LPS-challenged	1658:1671	arg1	macrophages					1679:1689	LPS-challenged THP-1 macrophages	1658:1689	LPS-challenged THP-1 macrophages	1658:1689	RESULTS Evaluation of TNF-α levels in LPS-challenged THP-1 macrophages evidenced a significant inhibition (>90%) upon treatment with the hydroethanolic extract obtained from X. aethiopica leaves at a concentration of 500 μg/mL.
31629028	2	106	theme	hydroethanolic	502:515	arg1	extract					517:523	a hydroethanolic extract	500:523	a hydroethanolic extract obtained from the leaves of Xylopia aethiopica (Dunal) A. Rich, (Annonaceae), used on the treatment of headache, muscular pain and rheumatic pain	500:669	As such, extracts obtained from a series of plants recorded in those surveys were screened for their anti-inflammatory properties, a hydroethanolic extract obtained from the leaves of Xylopia aethiopica (Dunal) A. Rich, (Annonaceae), used on the treatment of headache, muscular pain and rheumatic pain, scoring positively and being further investigated.
31629028	2	106	theme	hydroethanolic	502:515	arg1	properties					488:497	their anti-inflammatory properties	464:497	their anti-inflammatory properties	464:497	As such, extracts obtained from a series of plants recorded in those surveys were screened for their anti-inflammatory properties, a hydroethanolic extract obtained from the leaves of Xylopia aethiopica (Dunal) A. Rich, (Annonaceae), used on the treatment of headache, muscular pain and rheumatic pain, scoring positively and being further investigated.
31629028	0	107	theme	Xylopia	32:38	arg1	aethiopica					40:49	Xylopia aethiopica	32:49	Xylopia aethiopica	32:49	Anti-inflammatory properties of Xylopia aethiopica leaves: Interference with pro-inflammatory cytokines in THP-1-derived macrophages and flavonoid profiling.
31629028	1	108	theme	inflammatory	344:355	arg1	background					357:366	an inflammatory background	341:366	an inflammatory background	341:366	ETHNOPHARMACOLOGICAL RELEVANCE Ethnopharmacological surveys on Guinea-Bissauan flora reveal that several species are used to treat or ameliorate the symptomatology of conditions with an inflammatory background.
31629028	2	109	theme	anti-inflammatory	470:486	arg1	extract					517:523	a hydroethanolic extract	500:523	a hydroethanolic extract obtained from the leaves of Xylopia aethiopica (Dunal) A. Rich, (Annonaceae), used on the treatment of headache, muscular pain and rheumatic pain	500:669	As such, extracts obtained from a series of plants recorded in those surveys were screened for their anti-inflammatory properties, a hydroethanolic extract obtained from the leaves of Xylopia aethiopica (Dunal) A. Rich, (Annonaceae), used on the treatment of headache, muscular pain and rheumatic pain, scoring positively and being further investigated.
31629028	2	109	theme	anti-inflammatory	470:486	arg1	properties					488:497	their anti-inflammatory properties	464:497	their anti-inflammatory properties	464:497	As such, extracts obtained from a series of plants recorded in those surveys were screened for their anti-inflammatory properties, a hydroethanolic extract obtained from the leaves of Xylopia aethiopica (Dunal) A. Rich, (Annonaceae), used on the treatment of headache, muscular pain and rheumatic pain, scoring positively and being further investigated.
31629028	6	110	from	medium	1376:1381	arg1	quantification					1321:1334	the quantification	1317:1334	the quantification of TNF-α and IL-6 levels in the culture medium collected from LPS-activated THP-1-derived-macrophages	1317:1436	MATERIALS AND METHODS Interference with cytokines was evaluated by ELISA assay, through the quantification of TNF-α and IL-6 levels in the culture medium collected from LPS-activated THP-1-derived-macrophages.
31629028	13	111	theme	5-LOX	2505:2509	arg1	inhibition					2491:2500	the inhibition	2487:2500	the inhibition of 5-LOX	2487:2509	The main phenolic constituent, kaempferol-3-O-rutinoside (8), was found to significantly contribute to the anti-inflammatory effects, namely through the inhibition of 5-LOX.
31629028	10	112	from	250	1951:1953	arg1	levels					1941:1946	IL-6 levels	1936:1946	IL-6 levels at 250 and 500 μg/mL	1936:1967	Additional anti-inflammatory effects were recorded, including a significant decrease on IL-6 levels at 250 and 500 μg/mL.
31629028	12	113	theme	mono-O-glycosyl	2286:2300	arg1	4					2334:2334	4	2334:2334	4	2334:2334	Phenolic profiling allowed the identification and quantitation of eight constituents, including caffeoylquinic acids (1-3), mono-O-glycosylated flavonols (5-8), and the mono-O-glycosyl flavone luteolin-7-O-glucoside (4).
31629028	12	113	theme	mono-O-glycosyl	2286:2300	arg1	luteolin-7-O-glucoside					2310:2331	the mono-O-glycosyl flavone luteolin-7-O-glucoside	2282:2331	the mono-O-glycosyl flavone luteolin-7-O-glucoside (4)	2282:2335	Phenolic profiling allowed the identification and quantitation of eight constituents, including caffeoylquinic acids (1-3), mono-O-glycosylated flavonols (5-8), and the mono-O-glycosyl flavone luteolin-7-O-glucoside (4).
31629028	11	114	theme	significant	2028:2038	arg1	inhibition					2040:2049	significant inhibition	2028:2049	significant inhibition at concentrations ranging from 16 to 250 μg/mL (IC50 = 85 μg/mL)	2028:2114	The extract proved to be active towards 5-LOX, leading to significant inhibition at concentrations ranging from 16 to 250 μg/mL (IC50 = 85 μg/mL).
31629028	0	115	dep	leaves	51:56	arg1	Interference					59:70	Interference	59:70	Interference with pro-inflammatory cytokines in THP-1-derived macrophages and flavonoid profiling	59:155	Anti-inflammatory properties of Xylopia aethiopica leaves: Interference with pro-inflammatory cytokines in THP-1-derived macrophages and flavonoid profiling.
31629028	16	116	theme	observed	2778:2785	arg1	properties					2805:2814	the observed anti-inflammatory properties	2774:2814	the observed anti-inflammatory properties	2774:2814	Relevantly, the observed anti-inflammatory properties can stimulate further studies in order to fully unveil the therapeutic potential of the plant, namely as a source of phenolic compounds with a significant ability to interfere with conventional inflammatory targets.
31629028	12	117	dep	identification	2148:2161	arg1	the					2144:2146	the	2144:2146	the	2144:2146	Phenolic profiling allowed the identification and quantitation of eight constituents, including caffeoylquinic acids (1-3), mono-O-glycosylated flavonols (5-8), and the mono-O-glycosyl flavone luteolin-7-O-glucoside (4).
31629028	16	118	theme	inflammatory	3010:3021	arg1	targets					3023:3029	conventional inflammatory targets	2997:3029	conventional inflammatory targets	2997:3029	Relevantly, the observed anti-inflammatory properties can stimulate further studies in order to fully unveil the therapeutic potential of the plant, namely as a source of phenolic compounds with a significant ability to interfere with conventional inflammatory targets.
31629028	6	119	from	levels	1354:1359	arg1	medium					1376:1381	the culture medium	1364:1381	the culture medium collected from LPS-activated THP-1-derived-macrophages	1364:1436	MATERIALS AND METHODS Interference with cytokines was evaluated by ELISA assay, through the quantification of TNF-α and IL-6 levels in the culture medium collected from LPS-activated THP-1-derived-macrophages.
31629028	0	120	with	Interference	59:70	arg1	cytokines					94:102	pro-inflammatory cytokines	77:102	pro-inflammatory cytokines	77:102	Anti-inflammatory properties of Xylopia aethiopica leaves: Interference with pro-inflammatory cytokines in THP-1-derived macrophages and flavonoid profiling.
31495611	9	0	used	used	1478:1481	arg2	traits					1465:1470	MIR-derived traits	1453:1470	MIR-derived traits	1453:1470	In model 7, all previously described sources of information, but not MIR-derived traits, were used.
31495611	9	0	used	used	1478:1481	arg2	sources					1421:1427	all previously described sources	1396:1427	all previously described sources of information	1396:1442	In model 7, all previously described sources of information, but not MIR-derived traits, were used.
31495611	12	1	theme	operating	1925:1933	arg1	curve					1935:1939	the receiver operating curve	1912:1939	the receiver operating curve	1912:1939	The accuracy measures were sensitivity (i.e., the proportion of pregnant cows that were correctly classified), specificity (i.e., the proportion of open cows that were correctly classified), and area under the curve (AUC) for the receiver operating curve.
31495611	1	2	theme	likelihood	385:394	arg1	cows					360:363	cows	360:363	cows of good versus poor likelihood of conception to first insemination (i.e., pregnant vs. open)	360:456	The objective of this study was to investigate the potential of milk mid-infrared (MIR) spectroscopy, MIR-derived traits including milk composition, milk fatty acids, and blood metabolic profiles (fatty acids, β-hydroxybutyrate, and urea), and other on-farm data for discriminating cows of good versus poor likelihood of conception to first insemination (i.e., pregnant vs. open).
31495611	2	3	theme	cows	522:525	arg1	records					505:511	6,488 spectral and milk production records	470:511	6,488 spectral and milk production records of 2,987 cows from 19 commercial dairy herds across 3 Australian states	470:583	A total of 6,488 spectral and milk production records of 2,987 cows from 19 commercial dairy herds across 3 Australian states were used.
31495611	1	4	theme	conception	399:408	arg1	likelihood					385:394	good versus poor likelihood	368:394	good versus poor likelihood of conception to first insemination (i.e., pregnant vs. open)	368:456	The objective of this study was to investigate the potential of milk mid-infrared (MIR) spectroscopy, MIR-derived traits including milk composition, milk fatty acids, and blood metabolic profiles (fatty acids, β-hydroxybutyrate, and urea), and other on-farm data for discriminating cows of good versus poor likelihood of conception to first insemination (i.e., pregnant vs. open).
31495611	19	5	theme	0.02	3246:3249	arg1	accuracy					3220:3227	the prediction accuracy	3205:3227	the prediction accuracy of only 0.01 and 0.02	3205:3249	However, removing either the fertility estimated breeding value or animal genotype from model 7 resulted in a reduction of the prediction accuracy of only 0.01 and 0.02, respectively.
31495611	4	6	from	days	815:818	arg1	milk					792:795	milk	792:795	milk at herd test	792:808	Model 1 included milk production; concentrations of fat, protein, and lactose; somatic cell count; age at calving; days in milk at herd test; and days from calving to insemination.
31495611	4	6	from	days	815:818	arg1	test					805:808	herd test	800:808	herd test	800:808	Model 1 included milk production; concentrations of fat, protein, and lactose; somatic cell count; age at calving; days in milk at herd test; and days from calving to insemination.
31495611	4	7	theme	cell	756:759	arg1	count					761:765	somatic cell count	748:765	somatic cell count	748:765	Model 1 included milk production; concentrations of fat, protein, and lactose; somatic cell count; age at calving; days in milk at herd test; and days from calving to insemination.
31495611	5	8	dep	variables	887:895	arg1	addition					871:878	addition	871:878	addition	871:878	Model 2 included, in addition to the variables in model 1, milk fatty acids and blood metabolic profiles.
31495611	1	9	theme	first	413:417	arg1	insemination					419:430	first insemination	413:430	first insemination (i.e., pregnant vs. open)	413:456	The objective of this study was to investigate the potential of milk mid-infrared (MIR) spectroscopy, MIR-derived traits including milk composition, milk fatty acids, and blood metabolic profiles (fatty acids, β-hydroxybutyrate, and urea), and other on-farm data for discriminating cows of good versus poor likelihood of conception to first insemination (i.e., pregnant vs. open).
31495611	10	10	theme	discriminant	1538:1549	arg1	analysis					1551:1558	partial least squares discriminant analysis	1516:1558	partial least squares discriminant analysis	1516:1558	The models were developed using partial least squares discriminant analysis.
31495611	1	11	theme	MIR	161:163	arg1	spectroscopy					166:177	milk mid-infrared (MIR) spectroscopy	142:177	milk mid-infrared (MIR) spectroscopy	142:177	The objective of this study was to investigate the potential of milk mid-infrared (MIR) spectroscopy, MIR-derived traits including milk composition, milk fatty acids, and blood metabolic profiles (fatty acids, β-hydroxybutyrate, and urea), and other on-farm data for discriminating cows of good versus poor likelihood of conception to first insemination (i.e., pregnant vs. open).
31495611	14	12	theme	basic	2213:2217	arg1	information					2227:2237	basic on-farm information	2213:2237	basic on-farm information (model 1)	2213:2247	In the herd-by-herd external validation, using basic on-farm information (model 1) was not sufficient to classify good- and poor-fertility cows; the sensitivity, specificity, and AUC were around 0.66.
31495611	14	12	theme	basic	2213:2217	arg1	model					2240:2244	model 1	2240:2246	model 1	2240:2246	In the herd-by-herd external validation, using basic on-farm information (model 1) was not sufficient to classify good- and poor-fertility cows; the sensitivity, specificity, and AUC were around 0.66.
31495611	2	13	theme	dairy	546:550	arg1	herds					552:556	19 commercial dairy herds	532:556	19 commercial dairy herds across 3 Australian states	532:583	A total of 6,488 spectral and milk production records of 2,987 cows from 19 commercial dairy herds across 3 Australian states were used.
31495611	20	14	used	used	3343:3346	arg2	data					3329:3332	other on-farm data	3315:3332	other on-farm data	3315:3332	In conclusion, this study indicates that MIR and other on-farm data could be used to classify cows of good and poor likelihood of conception with promising accuracy.
31495611	20	14	used	used	3343:3346	arg2	MIR					3307:3309	MIR	3307:3309	MIR	3307:3309	In conclusion, this study indicates that MIR and other on-farm data could be used to classify cows of good and poor likelihood of conception with promising accuracy.
31495611	20	15	theme	good	3368:3371	arg1	likelihood					3382:3391	good and poor likelihood	3368:3391	good and poor likelihood of conception	3368:3405	In conclusion, this study indicates that MIR and other on-farm data could be used to classify cows of good and poor likelihood of conception with promising accuracy.
31495611	12	16	dep	sensitivity	1713:1723	arg1	proportion					1736:1745	the proportion	1732:1745	the proportion of pregnant cows that were correctly classified	1732:1793	The accuracy measures were sensitivity (i.e., the proportion of pregnant cows that were correctly classified), specificity (i.e., the proportion of open cows that were correctly classified), and area under the curve (AUC) for the receiver operating curve.
31495611	13	17	theme	random	2026:2031	arg1	cross-validation					2033:2048	10-fold random cross-validation	2018:2048	10-fold random cross-validation	2018:2048	The results showed that in all models, prediction accuracy obtained through 10-fold random cross-validation was higher than that of herd-by-herd external validation, with the difference in AUC ranging between 0.01 and 0.09.
31495611	5	18	from	variables	887:895	arg1	model					900:904	model 1	900:906	model 1	900:906	Model 2 included, in addition to the variables in model 1, milk fatty acids and blood metabolic profiles.
31495611	18	19	theme	estimated	2899:2907	arg1	value					2918:2922	estimated breeding value	2899:2922	estimated breeding value	2899:2922	Adding the fertility genomic estimated breeding value and animal genotypes (model 7) produced the highest prediction accuracy, with sensitivity, specificity, and AUC values of 0.75, 0.66, and 0.75, respectively.
31495611	17	20	from	traits	2827:2832	arg1	information					2798:2808	useful information	2791:2808	useful information from MIR-derived traits	2791:2832	The comparable prediction accuracies observed for models 3 and 4 mean that useful information from MIR-derived traits is already included in the spectra.
31495611	5	21	theme	fatty	914:918	arg1	acids					920:924	milk fatty acids	909:924	milk fatty acids	909:924	Model 2 included, in addition to the variables in model 1, milk fatty acids and blood metabolic profiles.
31495611	1	22	theme	MIR-derived	180:190	arg1	profiles					265:272	blood metabolic profiles	249:272	blood metabolic profiles (fatty acids, β-hydroxybutyrate, and urea)	249:315	The objective of this study was to investigate the potential of milk mid-infrared (MIR) spectroscopy, MIR-derived traits including milk composition, milk fatty acids, and blood metabolic profiles (fatty acids, β-hydroxybutyrate, and urea), and other on-farm data for discriminating cows of good versus poor likelihood of conception to first insemination (i.e., pregnant vs. open).
31495611	1	22	theme	MIR-derived	180:190	arg1	acids					238:242	milk fatty acids	227:242	milk fatty acids	227:242	The objective of this study was to investigate the potential of milk mid-infrared (MIR) spectroscopy, MIR-derived traits including milk composition, milk fatty acids, and blood metabolic profiles (fatty acids, β-hydroxybutyrate, and urea), and other on-farm data for discriminating cows of good versus poor likelihood of conception to first insemination (i.e., pregnant vs. open).
31495611	1	22	theme	MIR-derived	180:190	arg1	traits					192:197	MIR-derived traits	180:197	MIR-derived traits including milk composition, milk fatty acids, and blood metabolic profiles (fatty acids, β-hydroxybutyrate, and urea)	180:315	The objective of this study was to investigate the potential of milk mid-infrared (MIR) spectroscopy, MIR-derived traits including milk composition, milk fatty acids, and blood metabolic profiles (fatty acids, β-hydroxybutyrate, and urea), and other on-farm data for discriminating cows of good versus poor likelihood of conception to first insemination (i.e., pregnant vs. open).
31495611	1	22	theme	MIR-derived	180:190	arg1	composition					214:224	milk composition	209:224	milk composition	209:224	The objective of this study was to investigate the potential of milk mid-infrared (MIR) spectroscopy, MIR-derived traits including milk composition, milk fatty acids, and blood metabolic profiles (fatty acids, β-hydroxybutyrate, and urea), and other on-farm data for discriminating cows of good versus poor likelihood of conception to first insemination (i.e., pregnant vs. open).
31495611	12	23	theme	pregnant	1750:1757	arg1	cows					1759:1762	pregnant cows	1750:1762	pregnant cows that were correctly classified	1750:1793	The accuracy measures were sensitivity (i.e., the proportion of pregnant cows that were correctly classified), specificity (i.e., the proportion of open cows that were correctly classified), and area under the curve (AUC) for the receiver operating curve.
31495611	2	24	theme	6,488	470:474	arg1	spectral					476:483	6,488 spectral	470:483	6,488 spectral	470:483	A total of 6,488 spectral and milk production records of 2,987 cows from 19 commercial dairy herds across 3 Australian states were used.
31495611	8	25	theme	fertility	1227:1235	arg1	value					1264:1268	either fertility genomic estimated breeding value	1220:1268	either fertility genomic estimated breeding value	1220:1268	Model 5 and model 6 comprised model 4 and either fertility genomic estimated breeding value or principal components obtained from a genomic relationship matrix derived using animal genotypes, respectively.
31495611	14	26	from	validation	2195:2204	arg1	sufficient					2257:2266	sufficient	2257:2266	sufficient	2257:2266	In the herd-by-herd external validation, using basic on-farm information (model 1) was not sufficient to classify good- and poor-fertility cows; the sensitivity, specificity, and AUC were around 0.66.
31495611	11	27	theme	10-fold	1616:1622	arg1	cross-validation					1631:1646	10-fold random cross-validation	1616:1646	10-fold random cross-validation	1616:1646	The performance of each model was evaluated in 2 ways: 10-fold random cross-validation and herd-by-herd external validation.
31495611	11	27	theme	10-fold	1616:1622	arg1	ways					1610:1613	2 ways	1608:1613	2 ways: 10-fold random cross-validation and herd-by-herd external validation	1608:1683	The performance of each model was evaluated in 2 ways: 10-fold random cross-validation and herd-by-herd external validation.
31495611	4	28	from	test	805:808	arg1	count					761:765	somatic cell count	748:765	somatic cell count	748:765	Model 1 included milk production; concentrations of fat, protein, and lactose; somatic cell count; age at calving; days in milk at herd test; and days from calving to insemination.
31495611	4	28	from	test	805:808	arg1	days					784:787	days	784:787	days in milk at herd test	784:808	Model 1 included milk production; concentrations of fat, protein, and lactose; somatic cell count; age at calving; days in milk at herd test; and days from calving to insemination.
31495611	4	28	from	test	805:808	arg1	concentrations					703:716	concentrations	703:716	concentrations of fat, protein, and lactose	703:745	Model 1 included milk production; concentrations of fat, protein, and lactose; somatic cell count; age at calving; days in milk at herd test; and days from calving to insemination.
31495611	4	28	from	test	805:808	arg1	milk					792:795	milk	792:795	milk at herd test	792:808	Model 1 included milk production; concentrations of fat, protein, and lactose; somatic cell count; age at calving; days in milk at herd test; and days from calving to insemination.
31495611	4	28	from	test	805:808	arg1	days					815:818	days	815:818	days from calving to insemination	815:847	Model 1 included milk production; concentrations of fat, protein, and lactose; somatic cell count; age at calving; days in milk at herd test; and days from calving to insemination.
31495611	4	28	from	test	805:808	arg1	age					768:770	age	768:770	age at calving	768:781	Model 1 included milk production; concentrations of fat, protein, and lactose; somatic cell count; age at calving; days in milk at herd test; and days from calving to insemination.
31495611	19	29	theme	estimated	3121:3129	arg1	value					3140:3144	the fertility estimated breeding value	3107:3144	the fertility estimated breeding value	3107:3144	However, removing either the fertility estimated breeding value or animal genotype from model 7 resulted in a reduction of the prediction accuracy of only 0.01 and 0.02, respectively.
31495611	13	30	theme	external	2087:2094	arg1	validation					2096:2105	herd-by-herd external validation	2074:2105	herd-by-herd external validation	2074:2105	The results showed that in all models, prediction accuracy obtained through 10-fold random cross-validation was higher than that of herd-by-herd external validation, with the difference in AUC ranging between 0.01 and 0.09.
31495611	18	31	theme	animal	2928:2933	arg1	model					2946:2950	model 7	2946:2952	model 7	2946:2952	Adding the fertility genomic estimated breeding value and animal genotypes (model 7) produced the highest prediction accuracy, with sensitivity, specificity, and AUC values of 0.75, 0.66, and 0.75, respectively.
31495611	18	31	theme	animal	2928:2933	arg1	genotypes					2935:2943	animal genotypes	2928:2943	animal genotypes (model 7)	2928:2953	Adding the fertility genomic estimated breeding value and animal genotypes (model 7) produced the highest prediction accuracy, with sensitivity, specificity, and AUC values of 0.75, 0.66, and 0.75, respectively.
31495611	8	32	theme	estimated	1245:1253	arg1	value					1264:1268	either fertility genomic estimated breeding value	1220:1268	either fertility genomic estimated breeding value	1220:1268	Model 5 and model 6 comprised model 4 and either fertility genomic estimated breeding value or principal components obtained from a genomic relationship matrix derived using animal genotypes, respectively.
31495611	9	33	theme	MIR-derived	1453:1463	arg1	traits					1465:1470	MIR-derived traits	1453:1470	MIR-derived traits	1453:1470	In model 7, all previously described sources of information, but not MIR-derived traits, were used.
31495611	2	34	theme	production	494:503	arg1	records					505:511	6,488 spectral and milk production records	470:511	6,488 spectral and milk production records of 2,987 cows from 19 commercial dairy herds across 3 Australian states	470:583	A total of 6,488 spectral and milk production records of 2,987 cows from 19 commercial dairy herds across 3 Australian states were used.
31495611	19	35	theme	animal	3149:3154	arg1	genotype					3156:3163	animal genotype	3149:3163	animal genotype	3149:3163	However, removing either the fertility estimated breeding value or animal genotype from model 7 resulted in a reduction of the prediction accuracy of only 0.01 and 0.02, respectively.
31495611	8	36	theme	principal	1273:1281	arg1	components					1283:1292	principal components	1273:1292	principal components	1273:1292	Model 5 and model 6 comprised model 4 and either fertility genomic estimated breeding value or principal components obtained from a genomic relationship matrix derived using animal genotypes, respectively.
31495611	9	37	link	MIR-derived	1453:1463	arg1	traits					1465:1470	MIR-derived traits	1453:1470	MIR-derived traits	1453:1470	In model 7, all previously described sources of information, but not MIR-derived traits, were used.
31495611	12	38	theme	cows	1839:1842	arg1	proportion					1820:1829	the proportion	1816:1829	the proportion of open cows that were correctly classified	1816:1873	The accuracy measures were sensitivity (i.e., the proportion of pregnant cows that were correctly classified), specificity (i.e., the proportion of open cows that were correctly classified), and area under the curve (AUC) for the receiver operating curve.
31495611	0	39	theme	mid-infrared	51:62	arg1	spectroscopy					64:75	milk mid-infrared spectroscopy	46:75	milk mid-infrared spectroscopy	46:75	Classifying the fertility of dairy cows using milk mid-infrared spectroscopy.
31495611	19	40	theme	prediction	3209:3218	arg1	accuracy					3220:3227	the prediction accuracy	3205:3227	the prediction accuracy of only 0.01 and 0.02	3205:3249	However, removing either the fertility estimated breeding value or animal genotype from model 7 resulted in a reduction of the prediction accuracy of only 0.01 and 0.02, respectively.
31495611	18	41	theme	AUC	3032:3034	arg1	values					3036:3041	AUC values	3032:3041	AUC values of 0.75, 0.66, and 0.75, respectively	3032:3079	Adding the fertility genomic estimated breeding value and animal genotypes (model 7) produced the highest prediction accuracy, with sensitivity, specificity, and AUC values of 0.75, 0.66, and 0.75, respectively.
31495611	18	42	theme	breeding	2909:2916	arg1	value					2918:2922	estimated breeding value	2899:2922	estimated breeding value	2899:2922	Adding the fertility genomic estimated breeding value and animal genotypes (model 7) produced the highest prediction accuracy, with sensitivity, specificity, and AUC values of 0.75, 0.66, and 0.75, respectively.
31495611	16	43	theme	0.73	2670:2673	arg1	sensitivity					2626:2636	sensitivity	2626:2636	sensitivity	2626:2636	Incorporating MIR spectra into model 2 resulted in sensitivity, specificity, and AUC values of 0.73, 0.63, and 0.72, respectively (model 3).
31495611	16	43	theme	0.73	2670:2673	arg1	values					2660:2665	AUC values	2656:2665	AUC values of 0.73, 0.63, and 0.72	2656:2689	Incorporating MIR spectra into model 2 resulted in sensitivity, specificity, and AUC values of 0.73, 0.63, and 0.72, respectively (model 3).
31495611	16	43	theme	0.73	2670:2673	arg1	specificity					2639:2649	specificity	2639:2649	specificity	2639:2649	Incorporating MIR spectra into model 2 resulted in sensitivity, specificity, and AUC values of 0.73, 0.63, and 0.72, respectively (model 3).
31495611	4	44	from	concentrations	703:716	arg1	milk					792:795	milk	792:795	milk at herd test	792:808	Model 1 included milk production; concentrations of fat, protein, and lactose; somatic cell count; age at calving; days in milk at herd test; and days from calving to insemination.
31495611	4	44	from	concentrations	703:716	arg1	test					805:808	herd test	800:808	herd test	800:808	Model 1 included milk production; concentrations of fat, protein, and lactose; somatic cell count; age at calving; days in milk at herd test; and days from calving to insemination.
31495611	1	45	theme	mid-infrared	147:158	arg1	spectroscopy					166:177	milk mid-infrared (MIR) spectroscopy	142:177	milk mid-infrared (MIR) spectroscopy	142:177	The objective of this study was to investigate the potential of milk mid-infrared (MIR) spectroscopy, MIR-derived traits including milk composition, milk fatty acids, and blood metabolic profiles (fatty acids, β-hydroxybutyrate, and urea), and other on-farm data for discriminating cows of good versus poor likelihood of conception to first insemination (i.e., pregnant vs. open).
31495611	17	46	theme	MIR-derived	2815:2825	arg1	traits					2827:2832	MIR-derived traits	2815:2832	MIR-derived traits	2815:2832	The comparable prediction accuracies observed for models 3 and 4 mean that useful information from MIR-derived traits is already included in the spectra.
31495611	15	47	theme	fatty	2402:2406	arg1	model					2444:2448	model 2	2444:2450	model 2	2444:2450	Compared with model 1, adding milk fatty acids and blood metabolic profiles (model 2) increased the sensitivity, specificity, and AUC by 0.01, 0.02, and 0.02 unit, respectively (i.e., 0.65, 0.63, and 0.678).
31495611	15	47	theme	fatty	2402:2406	arg1	acids					2408:2412	milk fatty acids	2397:2412	milk fatty acids	2397:2412	Compared with model 1, adding milk fatty acids and blood metabolic profiles (model 2) increased the sensitivity, specificity, and AUC by 0.01, 0.02, and 0.02 unit, respectively (i.e., 0.65, 0.63, and 0.678).
31495611	5	48	theme	metabolic	936:944	arg1	profiles					946:953	blood metabolic profiles	930:953	blood metabolic profiles	930:953	Model 2 included, in addition to the variables in model 1, milk fatty acids and blood metabolic profiles.
31495611	8	49	theme	relationship	1318:1329	arg1	matrix					1331:1336	a genomic relationship matrix	1308:1336	a genomic relationship matrix derived using animal genotypes, respectively	1308:1381	Model 5 and model 6 comprised model 4 and either fertility genomic estimated breeding value or principal components obtained from a genomic relationship matrix derived using animal genotypes, respectively.
31495611	17	50	dep	models	2766:2771	arg1	4					2779:2779	4	2779:2779	4	2779:2779	The comparable prediction accuracies observed for models 3 and 4 mean that useful information from MIR-derived traits is already included in the spectra.
31495611	17	50	dep	models	2766:2771	arg1	3					2773:2773	3	2773:2773	3	2773:2773	The comparable prediction accuracies observed for models 3 and 4 mean that useful information from MIR-derived traits is already included in the spectra.
31495611	1	51	theme	other	322:326	arg1	profiles					265:272	blood metabolic profiles	249:272	blood metabolic profiles (fatty acids, β-hydroxybutyrate, and urea)	249:315	The objective of this study was to investigate the potential of milk mid-infrared (MIR) spectroscopy, MIR-derived traits including milk composition, milk fatty acids, and blood metabolic profiles (fatty acids, β-hydroxybutyrate, and urea), and other on-farm data for discriminating cows of good versus poor likelihood of conception to first insemination (i.e., pregnant vs. open).
31495611	1	51	theme	other	322:326	arg1	acids					238:242	milk fatty acids	227:242	milk fatty acids	227:242	The objective of this study was to investigate the potential of milk mid-infrared (MIR) spectroscopy, MIR-derived traits including milk composition, milk fatty acids, and blood metabolic profiles (fatty acids, β-hydroxybutyrate, and urea), and other on-farm data for discriminating cows of good versus poor likelihood of conception to first insemination (i.e., pregnant vs. open).
31495611	1	51	theme	other	322:326	arg1	data					336:339	other on-farm data	322:339	other on-farm data for discriminating cows of good versus poor likelihood of conception to first insemination (i.e., pregnant vs. open)	322:456	The objective of this study was to investigate the potential of milk mid-infrared (MIR) spectroscopy, MIR-derived traits including milk composition, milk fatty acids, and blood metabolic profiles (fatty acids, β-hydroxybutyrate, and urea), and other on-farm data for discriminating cows of good versus poor likelihood of conception to first insemination (i.e., pregnant vs. open).
31495611	1	51	theme	other	322:326	arg1	composition					214:224	milk composition	209:224	milk composition	209:224	The objective of this study was to investigate the potential of milk mid-infrared (MIR) spectroscopy, MIR-derived traits including milk composition, milk fatty acids, and blood metabolic profiles (fatty acids, β-hydroxybutyrate, and urea), and other on-farm data for discriminating cows of good versus poor likelihood of conception to first insemination (i.e., pregnant vs. open).
31495611	15	52	theme	blood	2418:2422	arg1	profiles					2434:2441	blood metabolic profiles	2418:2441	blood metabolic profiles	2418:2441	Compared with model 1, adding milk fatty acids and blood metabolic profiles (model 2) increased the sensitivity, specificity, and AUC by 0.01, 0.02, and 0.02 unit, respectively (i.e., 0.65, 0.63, and 0.678).
31495611	1	53	theme	blood	249:253	arg1	profiles					265:272	blood metabolic profiles	249:272	blood metabolic profiles (fatty acids, β-hydroxybutyrate, and urea)	249:315	The objective of this study was to investigate the potential of milk mid-infrared (MIR) spectroscopy, MIR-derived traits including milk composition, milk fatty acids, and blood metabolic profiles (fatty acids, β-hydroxybutyrate, and urea), and other on-farm data for discriminating cows of good versus poor likelihood of conception to first insemination (i.e., pregnant vs. open).
31495611	4	54	from	days	784:787	arg1	milk					792:795	milk	792:795	milk at herd test	792:808	Model 1 included milk production; concentrations of fat, protein, and lactose; somatic cell count; age at calving; days in milk at herd test; and days from calving to insemination.
31495611	4	54	from	days	784:787	arg1	test					805:808	herd test	800:808	herd test	800:808	Model 1 included milk production; concentrations of fat, protein, and lactose; somatic cell count; age at calving; days in milk at herd test; and days from calving to insemination.
31495611	18	55	theme	highest	2968:2974	arg1	accuracy					2987:2994	the highest prediction accuracy	2964:2994	the highest prediction accuracy	2964:2994	Adding the fertility genomic estimated breeding value and animal genotypes (model 7) produced the highest prediction accuracy, with sensitivity, specificity, and AUC values of 0.75, 0.66, and 0.75, respectively.
31495611	8	56	theme	animal	1352:1357	arg1	genotypes					1359:1367	animal genotypes	1352:1367	animal genotypes	1352:1367	Model 5 and model 6 comprised model 4 and either fertility genomic estimated breeding value or principal components obtained from a genomic relationship matrix derived using animal genotypes, respectively.
31495611	13	57	theme	prediction	1981:1990	arg1	accuracy					1992:1999	prediction accuracy	1981:1999	prediction accuracy obtained through 10-fold random cross-validation	1981:2048	The results showed that in all models, prediction accuracy obtained through 10-fold random cross-validation was higher than that of herd-by-herd external validation, with the difference in AUC ranging between 0.01 and 0.09.
31495611	1	58	theme	study	100:104	arg1	objective					82:90	The objective	78:90	The objective of this study	78:104	The objective of this study was to investigate the potential of milk mid-infrared (MIR) spectroscopy, MIR-derived traits including milk composition, milk fatty acids, and blood metabolic profiles (fatty acids, β-hydroxybutyrate, and urea), and other on-farm data for discriminating cows of good versus poor likelihood of conception to first insemination (i.e., pregnant vs. open).
31495611	20	59	theme	poor	3377:3380	arg1	likelihood					3382:3391	good and poor likelihood	3368:3391	good and poor likelihood of conception	3368:3405	In conclusion, this study indicates that MIR and other on-farm data could be used to classify cows of good and poor likelihood of conception with promising accuracy.
31495611	17	60	theme	prediction	2731:2740	arg1	accuracies					2742:2751	The comparable prediction accuracies	2716:2751	The comparable prediction accuracies	2716:2751	The comparable prediction accuracies observed for models 3 and 4 mean that useful information from MIR-derived traits is already included in the spectra.
31495611	20	61	theme	other	3315:3319	arg1	data					3329:3332	other on-farm data	3315:3332	other on-farm data	3315:3332	In conclusion, this study indicates that MIR and other on-farm data could be used to classify cows of good and poor likelihood of conception with promising accuracy.
31495611	0	62	theme	dairy	29:33	arg1	cows					35:38	dairy cows	29:38	dairy cows using milk mid-infrared spectroscopy	29:75	Classifying the fertility of dairy cows using milk mid-infrared spectroscopy.
31495611	1	63	theme	fatty	275:279	arg1	acids					281:285	fatty acids	275:285	fatty acids	275:285	The objective of this study was to investigate the potential of milk mid-infrared (MIR) spectroscopy, MIR-derived traits including milk composition, milk fatty acids, and blood metabolic profiles (fatty acids, β-hydroxybutyrate, and urea), and other on-farm data for discriminating cows of good versus poor likelihood of conception to first insemination (i.e., pregnant vs. open).
31495611	7	64	theme	blood	1108:1112	arg1	profiles					1124:1131	blood metabolic profiles	1108:1131	blood metabolic profiles	1108:1131	Fat, protein, and lactose percentages, milk fatty acids, and blood metabolic profiles were removed from model 3 to create model 4.
31495611	14	65	from	sufficient	2257:2266	arg1	validation					2195:2204	the herd-by-herd external validation	2169:2204	the herd-by-herd external validation	2169:2204	In the herd-by-herd external validation, using basic on-farm information (model 1) was not sufficient to classify good- and poor-fertility cows; the sensitivity, specificity, and AUC were around 0.66.
31495611	15	66	theme	metabolic	2424:2432	arg1	profiles					2434:2441	blood metabolic profiles	2418:2441	blood metabolic profiles	2418:2441	Compared with model 1, adding milk fatty acids and blood metabolic profiles (model 2) increased the sensitivity, specificity, and AUC by 0.01, 0.02, and 0.02 unit, respectively (i.e., 0.65, 0.63, and 0.678).
31495611	2	67	used	used	590:593	arg2	total					461:465	A total	459:465	A total of 6,488 spectral and milk production records of 2,987 cows from 19 commercial dairy herds across 3 Australian states	459:583	A total of 6,488 spectral and milk production records of 2,987 cows from 19 commercial dairy herds across 3 Australian states were used.
31495611	9	68	theme	described	1411:1419	arg1	sources					1421:1427	all previously described sources	1396:1427	all previously described sources of information	1396:1442	In model 7, all previously described sources of information, but not MIR-derived traits, were used.
31495611	2	69	from	herds	552:556	arg1	records					505:511	6,488 spectral and milk production records	470:511	6,488 spectral and milk production records of 2,987 cows from 19 commercial dairy herds across 3 Australian states	470:583	A total of 6,488 spectral and milk production records of 2,987 cows from 19 commercial dairy herds across 3 Australian states were used.
31495611	2	69	from	herds	552:556	arg1	total					461:465	A total	459:465	A total of 6,488 spectral and milk production records of 2,987 cows from 19 commercial dairy herds across 3 Australian states	459:583	A total of 6,488 spectral and milk production records of 2,987 cows from 19 commercial dairy herds across 3 Australian states were used.
31495611	17	70	theme	models	2766:2771	arg1	mean					2781:2784	models 3 and 4 mean	2766:2784	models 3 and 4 mean	2766:2784	The comparable prediction accuracies observed for models 3 and 4 mean that useful information from MIR-derived traits is already included in the spectra.
31495611	4	71	theme	fat	721:723	arg1	count					761:765	somatic cell count	748:765	somatic cell count	748:765	Model 1 included milk production; concentrations of fat, protein, and lactose; somatic cell count; age at calving; days in milk at herd test; and days from calving to insemination.
31495611	4	71	theme	fat	721:723	arg1	days					784:787	days	784:787	days in milk at herd test	784:808	Model 1 included milk production; concentrations of fat, protein, and lactose; somatic cell count; age at calving; days in milk at herd test; and days from calving to insemination.
31495611	4	71	theme	fat	721:723	arg1	days					815:818	days	815:818	days from calving to insemination	815:847	Model 1 included milk production; concentrations of fat, protein, and lactose; somatic cell count; age at calving; days in milk at herd test; and days from calving to insemination.
31495611	4	71	theme	fat	721:723	arg1	age					768:770	age	768:770	age at calving	768:781	Model 1 included milk production; concentrations of fat, protein, and lactose; somatic cell count; age at calving; days in milk at herd test; and days from calving to insemination.
31495611	4	71	theme	fat	721:723	arg1	concentrations					703:716	concentrations	703:716	concentrations of fat, protein, and lactose	703:745	Model 1 included milk production; concentrations of fat, protein, and lactose; somatic cell count; age at calving; days in milk at herd test; and days from calving to insemination.
31495611	14	72	theme	external	2186:2193	arg1	validation					2195:2204	the herd-by-herd external validation	2169:2204	the herd-by-herd external validation	2169:2204	In the herd-by-herd external validation, using basic on-farm information (model 1) was not sufficient to classify good- and poor-fertility cows; the sensitivity, specificity, and AUC were around 0.66.
31495611	1	73	theme	milk	209:212	arg1	composition					214:224	milk composition	209:224	milk composition	209:224	The objective of this study was to investigate the potential of milk mid-infrared (MIR) spectroscopy, MIR-derived traits including milk composition, milk fatty acids, and blood metabolic profiles (fatty acids, β-hydroxybutyrate, and urea), and other on-farm data for discriminating cows of good versus poor likelihood of conception to first insemination (i.e., pregnant vs. open).
31495611	7	74	theme	fatty	1091:1095	arg1	acids					1097:1101	milk fatty acids	1086:1101	milk fatty acids	1086:1101	Fat, protein, and lactose percentages, milk fatty acids, and blood metabolic profiles were removed from model 3 to create model 4.
31495611	4	75	theme	protein	726:732	arg1	count					761:765	somatic cell count	748:765	somatic cell count	748:765	Model 1 included milk production; concentrations of fat, protein, and lactose; somatic cell count; age at calving; days in milk at herd test; and days from calving to insemination.
31495611	4	75	theme	protein	726:732	arg1	days					784:787	days	784:787	days in milk at herd test	784:808	Model 1 included milk production; concentrations of fat, protein, and lactose; somatic cell count; age at calving; days in milk at herd test; and days from calving to insemination.
31495611	4	75	theme	protein	726:732	arg1	days					815:818	days	815:818	days from calving to insemination	815:847	Model 1 included milk production; concentrations of fat, protein, and lactose; somatic cell count; age at calving; days in milk at herd test; and days from calving to insemination.
31495611	4	75	theme	protein	726:732	arg1	age					768:770	age	768:770	age at calving	768:781	Model 1 included milk production; concentrations of fat, protein, and lactose; somatic cell count; age at calving; days in milk at herd test; and days from calving to insemination.
31495611	4	75	theme	protein	726:732	arg1	concentrations					703:716	concentrations	703:716	concentrations of fat, protein, and lactose	703:745	Model 1 included milk production; concentrations of fat, protein, and lactose; somatic cell count; age at calving; days in milk at herd test; and days from calving to insemination.
31495611	10	76	theme	squares	1530:1536	arg1	analysis					1551:1558	partial least squares discriminant analysis	1516:1558	partial least squares discriminant analysis	1516:1558	The models were developed using partial least squares discriminant analysis.
31495611	16	77	theme	AUC	2656:2658	arg1	values					2660:2665	AUC values	2656:2665	AUC values of 0.73, 0.63, and 0.72	2656:2689	Incorporating MIR spectra into model 2 resulted in sensitivity, specificity, and AUC values of 0.73, 0.63, and 0.72, respectively (model 3).
31495611	1	78	theme	poor	380:383	arg1	likelihood					385:394	good versus poor likelihood	368:394	good versus poor likelihood of conception to first insemination (i.e., pregnant vs. open)	368:456	The objective of this study was to investigate the potential of milk mid-infrared (MIR) spectroscopy, MIR-derived traits including milk composition, milk fatty acids, and blood metabolic profiles (fatty acids, β-hydroxybutyrate, and urea), and other on-farm data for discriminating cows of good versus poor likelihood of conception to first insemination (i.e., pregnant vs. open).
31495611	3	79	theme	explanatory	631:641	arg1	variables					643:651	different explanatory variables	621:651	different explanatory variables	621:651	Seven models, comprising different explanatory variables, were examined.
31495611	17	80	theme	useful	2791:2796	arg1	information					2798:2808	useful information	2791:2808	useful information from MIR-derived traits	2791:2832	The comparable prediction accuracies observed for models 3 and 4 mean that useful information from MIR-derived traits is already included in the spectra.
31495611	10	81	theme	partial	1516:1522	arg1	analysis					1551:1558	partial least squares discriminant analysis	1516:1558	partial least squares discriminant analysis	1516:1558	The models were developed using partial least squares discriminant analysis.
31495611	1	82	theme	fatty	232:236	arg1	acids					238:242	milk fatty acids	227:242	milk fatty acids	227:242	The objective of this study was to investigate the potential of milk mid-infrared (MIR) spectroscopy, MIR-derived traits including milk composition, milk fatty acids, and blood metabolic profiles (fatty acids, β-hydroxybutyrate, and urea), and other on-farm data for discriminating cows of good versus poor likelihood of conception to first insemination (i.e., pregnant vs. open).
31495611	7	83	theme	lactose	1065:1071	arg1	percentages					1073:1083	Fat, protein, and lactose percentages	1047:1083	Fat, protein, and lactose percentages	1047:1083	Fat, protein, and lactose percentages, milk fatty acids, and blood metabolic profiles were removed from model 3 to create model 4.
31495611	16	84	theme	MIR	2589:2591	arg1	spectra					2593:2599	MIR spectra	2589:2599	MIR spectra	2589:2599	Incorporating MIR spectra into model 2 resulted in sensitivity, specificity, and AUC values of 0.73, 0.63, and 0.72, respectively (model 3).
31495611	15	85	dep	0.65	2551:2554	arg1	i.e.					2545:2548	i.e., 0.65, 0.63, and 0.678	2545:2571	i.e.	2545:2548	Compared with model 1, adding milk fatty acids and blood metabolic profiles (model 2) increased the sensitivity, specificity, and AUC by 0.01, 0.02, and 0.02 unit, respectively (i.e., 0.65, 0.63, and 0.678).
31495611	11	86	theme	herd-by-herd	1652:1663	arg1	ways					1610:1613	2 ways	1608:1613	2 ways: 10-fold random cross-validation and herd-by-herd external validation	1608:1683	The performance of each model was evaluated in 2 ways: 10-fold random cross-validation and herd-by-herd external validation.
31495611	11	86	theme	herd-by-herd	1652:1663	arg1	validation					1674:1683	herd-by-herd external validation	1652:1683	herd-by-herd external validation	1652:1683	The performance of each model was evaluated in 2 ways: 10-fold random cross-validation and herd-by-herd external validation.
31495611	14	87	theme	on-farm	2219:2225	arg1	information					2227:2237	basic on-farm information	2213:2237	basic on-farm information (model 1)	2213:2247	In the herd-by-herd external validation, using basic on-farm information (model 1) was not sufficient to classify good- and poor-fertility cows; the sensitivity, specificity, and AUC were around 0.66.
31495611	14	87	theme	on-farm	2219:2225	arg1	model					2240:2244	model 1	2240:2246	model 1	2240:2246	In the herd-by-herd external validation, using basic on-farm information (model 1) was not sufficient to classify good- and poor-fertility cows; the sensitivity, specificity, and AUC were around 0.66.
31495611	4	88	theme	somatic	748:754	arg1	count					761:765	somatic cell count	748:765	somatic cell count	748:765	Model 1 included milk production; concentrations of fat, protein, and lactose; somatic cell count; age at calving; days in milk at herd test; and days from calving to insemination.
31495611	18	89	theme	0.75	3062:3065	arg1	values					3036:3041	AUC values	3032:3041	AUC values of 0.75, 0.66, and 0.75, respectively	3032:3079	Adding the fertility genomic estimated breeding value and animal genotypes (model 7) produced the highest prediction accuracy, with sensitivity, specificity, and AUC values of 0.75, 0.66, and 0.75, respectively.
31495611	18	89	theme	0.75	3062:3065	arg1	specificity					3015:3025	specificity	3015:3025	specificity	3015:3025	Adding the fertility genomic estimated breeding value and animal genotypes (model 7) produced the highest prediction accuracy, with sensitivity, specificity, and AUC values of 0.75, 0.66, and 0.75, respectively.
31495611	18	89	theme	0.75	3062:3065	arg1	sensitivity					3002:3012	sensitivity	3002:3012	sensitivity	3002:3012	Adding the fertility genomic estimated breeding value and animal genotypes (model 7) produced the highest prediction accuracy, with sensitivity, specificity, and AUC values of 0.75, 0.66, and 0.75, respectively.
31495611	2	90	theme	commercial	535:544	arg1	herds					552:556	19 commercial dairy herds	532:556	19 commercial dairy herds across 3 Australian states	532:583	A total of 6,488 spectral and milk production records of 2,987 cows from 19 commercial dairy herds across 3 Australian states were used.
31495611	20	91	theme	on-farm	3321:3327	arg1	data					3329:3332	other on-farm data	3315:3332	other on-farm data	3315:3332	In conclusion, this study indicates that MIR and other on-farm data could be used to classify cows of good and poor likelihood of conception with promising accuracy.
31495611	17	92	link	MIR-derived	2815:2825	arg1	traits					2827:2832	MIR-derived traits	2815:2832	MIR-derived traits	2815:2832	The comparable prediction accuracies observed for models 3 and 4 mean that useful information from MIR-derived traits is already included in the spectra.
31495611	2	93	theme	spectral	476:483	arg1	records					505:511	6,488 spectral and milk production records	470:511	6,488 spectral and milk production records of 2,987 cows from 19 commercial dairy herds across 3 Australian states	470:583	A total of 6,488 spectral and milk production records of 2,987 cows from 19 commercial dairy herds across 3 Australian states were used.
31495611	8	94	theme	genomic	1237:1243	arg1	value					1264:1268	either fertility genomic estimated breeding value	1220:1268	either fertility genomic estimated breeding value	1220:1268	Model 5 and model 6 comprised model 4 and either fertility genomic estimated breeding value or principal components obtained from a genomic relationship matrix derived using animal genotypes, respectively.
31495611	6	95	theme	first	990:994	arg1	insemination					996:1007	first insemination	990:1007	first insemination	990:1007	The MIR spectrum collected before first insemination was added to model 2 to form model 3.
31495611	12	96	dep	proportion	1736:1745	arg1	i.e.					1726:1729	i.e.	1726:1729	i.e.	1726:1729	The accuracy measures were sensitivity (i.e., the proportion of pregnant cows that were correctly classified), specificity (i.e., the proportion of open cows that were correctly classified), and area under the curve (AUC) for the receiver operating curve.
31495611	19	97	theme	accuracy	3220:3227	arg1	reduction					3192:3200	a reduction	3190:3200	a reduction of the prediction accuracy of only 0.01 and 0.02	3190:3249	However, removing either the fertility estimated breeding value or animal genotype from model 7 resulted in a reduction of the prediction accuracy of only 0.01 and 0.02, respectively.
31495611	19	98	theme	fertility	3111:3119	arg1	value					3140:3144	the fertility estimated breeding value	3107:3144	the fertility estimated breeding value	3107:3144	However, removing either the fertility estimated breeding value or animal genotype from model 7 resulted in a reduction of the prediction accuracy of only 0.01 and 0.02, respectively.
31495611	13	99	theme	herd-by-herd	2074:2085	arg1	validation					2096:2105	herd-by-herd external validation	2074:2105	herd-by-herd external validation	2074:2105	The results showed that in all models, prediction accuracy obtained through 10-fold random cross-validation was higher than that of herd-by-herd external validation, with the difference in AUC ranging between 0.01 and 0.09.
31495611	8	100	theme	breeding	1255:1262	arg1	value					1264:1268	either fertility genomic estimated breeding value	1220:1268	either fertility genomic estimated breeding value	1220:1268	Model 5 and model 6 comprised model 4 and either fertility genomic estimated breeding value or principal components obtained from a genomic relationship matrix derived using animal genotypes, respectively.
31495611	11	101	theme	random	1624:1629	arg1	cross-validation					1631:1646	10-fold random cross-validation	1616:1646	10-fold random cross-validation	1616:1646	The performance of each model was evaluated in 2 ways: 10-fold random cross-validation and herd-by-herd external validation.
31495611	11	101	theme	random	1624:1629	arg1	ways					1610:1613	2 ways	1608:1613	2 ways: 10-fold random cross-validation and herd-by-herd external validation	1608:1683	The performance of each model was evaluated in 2 ways: 10-fold random cross-validation and herd-by-herd external validation.
31495611	5	102	theme	blood	930:934	arg1	profiles					946:953	blood metabolic profiles	930:953	blood metabolic profiles	930:953	Model 2 included, in addition to the variables in model 1, milk fatty acids and blood metabolic profiles.
31495611	12	103	theme	cows	1759:1762	arg1	proportion					1736:1745	the proportion	1732:1745	the proportion of pregnant cows that were correctly classified	1732:1793	The accuracy measures were sensitivity (i.e., the proportion of pregnant cows that were correctly classified), specificity (i.e., the proportion of open cows that were correctly classified), and area under the curve (AUC) for the receiver operating curve.
31495611	2	104	theme	records	505:511	arg1	total					461:465	A total	459:465	A total of 6,488 spectral and milk production records of 2,987 cows from 19 commercial dairy herds across 3 Australian states	459:583	A total of 6,488 spectral and milk production records of 2,987 cows from 19 commercial dairy herds across 3 Australian states were used.
31495611	20	105	theme	conception	3396:3405	arg1	likelihood					3382:3391	good and poor likelihood	3368:3391	good and poor likelihood of conception	3368:3405	In conclusion, this study indicates that MIR and other on-farm data could be used to classify cows of good and poor likelihood of conception with promising accuracy.
31495611	1	106	theme	good	368:371	arg1	likelihood					385:394	good versus poor likelihood	368:394	good versus poor likelihood of conception to first insemination (i.e., pregnant vs. open)	368:456	The objective of this study was to investigate the potential of milk mid-infrared (MIR) spectroscopy, MIR-derived traits including milk composition, milk fatty acids, and blood metabolic profiles (fatty acids, β-hydroxybutyrate, and urea), and other on-farm data for discriminating cows of good versus poor likelihood of conception to first insemination (i.e., pregnant vs. open).
31495611	2	107	theme	milk	489:492	arg1	production					494:503	milk production	489:503	milk production	489:503	A total of 6,488 spectral and milk production records of 2,987 cows from 19 commercial dairy herds across 3 Australian states were used.
31495611	2	108	from	total	461:465	arg1	herds					552:556	19 commercial dairy herds	532:556	19 commercial dairy herds across 3 Australian states	532:583	A total of 6,488 spectral and milk production records of 2,987 cows from 19 commercial dairy herds across 3 Australian states were used.
31495611	20	109	theme	promising	3412:3420	arg1	accuracy					3422:3429	promising accuracy	3412:3429	promising accuracy	3412:3429	In conclusion, this study indicates that MIR and other on-farm data could be used to classify cows of good and poor likelihood of conception with promising accuracy.
31495611	7	110	theme	protein	1052:1058	arg1	percentages					1073:1083	Fat, protein, and lactose percentages	1047:1083	Fat, protein, and lactose percentages	1047:1083	Fat, protein, and lactose percentages, milk fatty acids, and blood metabolic profiles were removed from model 3 to create model 4.
31495611	5	111	theme	milk	909:912	arg1	acids					920:924	milk fatty acids	909:924	milk fatty acids	909:924	Model 2 included, in addition to the variables in model 1, milk fatty acids and blood metabolic profiles.
31495611	19	112	theme	breeding	3131:3138	arg1	value					3140:3144	the fertility estimated breeding value	3107:3144	the fertility estimated breeding value	3107:3144	However, removing either the fertility estimated breeding value or animal genotype from model 7 resulted in a reduction of the prediction accuracy of only 0.01 and 0.02, respectively.
31495611	1	113	dep	profiles	265:272	arg1	urea					311:314	urea	311:314	urea	311:314	The objective of this study was to investigate the potential of milk mid-infrared (MIR) spectroscopy, MIR-derived traits including milk composition, milk fatty acids, and blood metabolic profiles (fatty acids, β-hydroxybutyrate, and urea), and other on-farm data for discriminating cows of good versus poor likelihood of conception to first insemination (i.e., pregnant vs. open).
31495611	1	113	dep	profiles	265:272	arg1	acids					281:285	fatty acids	275:285	fatty acids	275:285	The objective of this study was to investigate the potential of milk mid-infrared (MIR) spectroscopy, MIR-derived traits including milk composition, milk fatty acids, and blood metabolic profiles (fatty acids, β-hydroxybutyrate, and urea), and other on-farm data for discriminating cows of good versus poor likelihood of conception to first insemination (i.e., pregnant vs. open).
31495611	1	113	dep	profiles	265:272	arg1	β-hydroxybutyrate					288:304	β-hydroxybutyrate	288:304	β-hydroxybutyrate	288:304	The objective of this study was to investigate the potential of milk mid-infrared (MIR) spectroscopy, MIR-derived traits including milk composition, milk fatty acids, and blood metabolic profiles (fatty acids, β-hydroxybutyrate, and urea), and other on-farm data for discriminating cows of good versus poor likelihood of conception to first insemination (i.e., pregnant vs. open).
31495611	6	114	theme	form	1033:1036	arg1	model					1038:1042	form model 3	1033:1044	form model 3	1033:1044	The MIR spectrum collected before first insemination was added to model 2 to form model 3.
31495611	12	115	theme	open	1834:1837	arg1	cows					1839:1842	open cows	1834:1842	open cows that were correctly classified	1834:1873	The accuracy measures were sensitivity (i.e., the proportion of pregnant cows that were correctly classified), specificity (i.e., the proportion of open cows that were correctly classified), and area under the curve (AUC) for the receiver operating curve.
31495611	16	116	theme	0.63	2676:2679	arg1	sensitivity					2626:2636	sensitivity	2626:2636	sensitivity	2626:2636	Incorporating MIR spectra into model 2 resulted in sensitivity, specificity, and AUC values of 0.73, 0.63, and 0.72, respectively (model 3).
31495611	16	116	theme	0.63	2676:2679	arg1	values					2660:2665	AUC values	2656:2665	AUC values of 0.73, 0.63, and 0.72	2656:2689	Incorporating MIR spectra into model 2 resulted in sensitivity, specificity, and AUC values of 0.73, 0.63, and 0.72, respectively (model 3).
31495611	16	116	theme	0.63	2676:2679	arg1	specificity					2639:2649	specificity	2639:2649	specificity	2639:2649	Incorporating MIR spectra into model 2 resulted in sensitivity, specificity, and AUC values of 0.73, 0.63, and 0.72, respectively (model 3).
31495611	7	117	theme	Fat	1047:1049	arg1	percentages					1073:1083	Fat, protein, and lactose percentages	1047:1083	Fat, protein, and lactose percentages	1047:1083	Fat, protein, and lactose percentages, milk fatty acids, and blood metabolic profiles were removed from model 3 to create model 4.
31495611	4	118	from	calving	775:781	arg1	count					761:765	somatic cell count	748:765	somatic cell count	748:765	Model 1 included milk production; concentrations of fat, protein, and lactose; somatic cell count; age at calving; days in milk at herd test; and days from calving to insemination.
31495611	4	118	from	calving	775:781	arg1	days					784:787	days	784:787	days in milk at herd test	784:808	Model 1 included milk production; concentrations of fat, protein, and lactose; somatic cell count; age at calving; days in milk at herd test; and days from calving to insemination.
31495611	4	118	from	calving	775:781	arg1	days					815:818	days	815:818	days from calving to insemination	815:847	Model 1 included milk production; concentrations of fat, protein, and lactose; somatic cell count; age at calving; days in milk at herd test; and days from calving to insemination.
31495611	4	118	from	calving	775:781	arg1	age					768:770	age	768:770	age at calving	768:781	Model 1 included milk production; concentrations of fat, protein, and lactose; somatic cell count; age at calving; days in milk at herd test; and days from calving to insemination.
31495611	4	118	from	calving	775:781	arg1	concentrations					703:716	concentrations	703:716	concentrations of fat, protein, and lactose	703:745	Model 1 included milk production; concentrations of fat, protein, and lactose; somatic cell count; age at calving; days in milk at herd test; and days from calving to insemination.
31495611	1	119	theme	milk	142:145	arg1	spectroscopy					166:177	milk mid-infrared (MIR) spectroscopy	142:177	milk mid-infrared (MIR) spectroscopy	142:177	The objective of this study was to investigate the potential of milk mid-infrared (MIR) spectroscopy, MIR-derived traits including milk composition, milk fatty acids, and blood metabolic profiles (fatty acids, β-hydroxybutyrate, and urea), and other on-farm data for discriminating cows of good versus poor likelihood of conception to first insemination (i.e., pregnant vs. open).
31495611	11	120	dep	ways	1610:1613	arg1	cross-validation					1631:1646	10-fold random cross-validation	1616:1646	10-fold random cross-validation	1616:1646	The performance of each model was evaluated in 2 ways: 10-fold random cross-validation and herd-by-herd external validation.
31495611	11	120	dep	ways	1610:1613	arg1	ways					1610:1613	2 ways	1608:1613	2 ways: 10-fold random cross-validation and herd-by-herd external validation	1608:1683	The performance of each model was evaluated in 2 ways: 10-fold random cross-validation and herd-by-herd external validation.
31495611	11	120	dep	ways	1610:1613	arg1	validation					1674:1683	herd-by-herd external validation	1652:1683	herd-by-herd external validation	1652:1683	The performance of each model was evaluated in 2 ways: 10-fold random cross-validation and herd-by-herd external validation.
31495611	18	121	theme	genomic	2891:2897	arg1	value					2918:2922	estimated breeding value	2899:2922	estimated breeding value	2899:2922	Adding the fertility genomic estimated breeding value and animal genotypes (model 7) produced the highest prediction accuracy, with sensitivity, specificity, and AUC values of 0.75, 0.66, and 0.75, respectively.
31495611	8	122	theme	genomic	1310:1316	arg1	matrix					1331:1336	a genomic relationship matrix	1308:1336	a genomic relationship matrix derived using animal genotypes, respectively	1308:1381	Model 5 and model 6 comprised model 4 and either fertility genomic estimated breeding value or principal components obtained from a genomic relationship matrix derived using animal genotypes, respectively.
31495611	15	123	dep	increased	2453:2461	arg1	0.65					2551:2554	0.65	2551:2554	0.65	2551:2554	Compared with model 1, adding milk fatty acids and blood metabolic profiles (model 2) increased the sensitivity, specificity, and AUC by 0.01, 0.02, and 0.02 unit, respectively (i.e., 0.65, 0.63, and 0.678).
31495611	15	123	dep	increased	2453:2461	arg1	0.63					2557:2560	0.63	2557:2560	0.63	2557:2560	Compared with model 1, adding milk fatty acids and blood metabolic profiles (model 2) increased the sensitivity, specificity, and AUC by 0.01, 0.02, and 0.02 unit, respectively (i.e., 0.65, 0.63, and 0.678).
31495611	15	123	dep	increased	2453:2461	arg1	0.678					2567:2571	0.678	2567:2571	0.678	2567:2571	Compared with model 1, adding milk fatty acids and blood metabolic profiles (model 2) increased the sensitivity, specificity, and AUC by 0.01, 0.02, and 0.02 unit, respectively (i.e., 0.65, 0.63, and 0.678).
31495611	20	124	theme	likelihood	3382:3391	arg1	cows					3360:3363	cows	3360:3363	cows of good and poor likelihood of conception with promising accuracy	3360:3429	In conclusion, this study indicates that MIR and other on-farm data could be used to classify cows of good and poor likelihood of conception with promising accuracy.
31495611	11	125	theme	model	1585:1589	arg1	performance					1565:1575	The performance	1561:1575	The performance of each model	1561:1589	The performance of each model was evaluated in 2 ways: 10-fold random cross-validation and herd-by-herd external validation.
31495611	15	126	theme	milk	2397:2400	arg1	model					2444:2448	model 2	2444:2450	model 2	2444:2450	Compared with model 1, adding milk fatty acids and blood metabolic profiles (model 2) increased the sensitivity, specificity, and AUC by 0.01, 0.02, and 0.02 unit, respectively (i.e., 0.65, 0.63, and 0.678).
31495611	15	126	theme	milk	2397:2400	arg1	acids					2408:2412	milk fatty acids	2397:2412	milk fatty acids	2397:2412	Compared with model 1, adding milk fatty acids and blood metabolic profiles (model 2) increased the sensitivity, specificity, and AUC by 0.01, 0.02, and 0.02 unit, respectively (i.e., 0.65, 0.63, and 0.678).
31495611	4	127	from	age	768:770	arg1	milk					792:795	milk	792:795	milk at herd test	792:808	Model 1 included milk production; concentrations of fat, protein, and lactose; somatic cell count; age at calving; days in milk at herd test; and days from calving to insemination.
31495611	4	127	from	age	768:770	arg1	test					805:808	herd test	800:808	herd test	800:808	Model 1 included milk production; concentrations of fat, protein, and lactose; somatic cell count; age at calving; days in milk at herd test; and days from calving to insemination.
31495611	1	128	dep	insemination	419:430	arg1	open					452:455	open	452:455	open	452:455	The objective of this study was to investigate the potential of milk mid-infrared (MIR) spectroscopy, MIR-derived traits including milk composition, milk fatty acids, and blood metabolic profiles (fatty acids, β-hydroxybutyrate, and urea), and other on-farm data for discriminating cows of good versus poor likelihood of conception to first insemination (i.e., pregnant vs. open).
31495611	1	128	dep	insemination	419:430	arg1	pregnant					439:446	pregnant	439:446	pregnant	439:446	The objective of this study was to investigate the potential of milk mid-infrared (MIR) spectroscopy, MIR-derived traits including milk composition, milk fatty acids, and blood metabolic profiles (fatty acids, β-hydroxybutyrate, and urea), and other on-farm data for discriminating cows of good versus poor likelihood of conception to first insemination (i.e., pregnant vs. open).
31495611	18	129	theme	0.66	3052:3055	arg1	values					3036:3041	AUC values	3032:3041	AUC values of 0.75, 0.66, and 0.75, respectively	3032:3079	Adding the fertility genomic estimated breeding value and animal genotypes (model 7) produced the highest prediction accuracy, with sensitivity, specificity, and AUC values of 0.75, 0.66, and 0.75, respectively.
31495611	18	129	theme	0.66	3052:3055	arg1	specificity					3015:3025	specificity	3015:3025	specificity	3015:3025	Adding the fertility genomic estimated breeding value and animal genotypes (model 7) produced the highest prediction accuracy, with sensitivity, specificity, and AUC values of 0.75, 0.66, and 0.75, respectively.
31495611	18	129	theme	0.66	3052:3055	arg1	sensitivity					3002:3012	sensitivity	3002:3012	sensitivity	3002:3012	Adding the fertility genomic estimated breeding value and animal genotypes (model 7) produced the highest prediction accuracy, with sensitivity, specificity, and AUC values of 0.75, 0.66, and 0.75, respectively.
31495611	1	130	link	MIR-derived	180:190	arg1	profiles					265:272	blood metabolic profiles	249:272	blood metabolic profiles (fatty acids, β-hydroxybutyrate, and urea)	249:315	The objective of this study was to investigate the potential of milk mid-infrared (MIR) spectroscopy, MIR-derived traits including milk composition, milk fatty acids, and blood metabolic profiles (fatty acids, β-hydroxybutyrate, and urea), and other on-farm data for discriminating cows of good versus poor likelihood of conception to first insemination (i.e., pregnant vs. open).
31495611	1	130	link	MIR-derived	180:190	arg1	acids					238:242	milk fatty acids	227:242	milk fatty acids	227:242	The objective of this study was to investigate the potential of milk mid-infrared (MIR) spectroscopy, MIR-derived traits including milk composition, milk fatty acids, and blood metabolic profiles (fatty acids, β-hydroxybutyrate, and urea), and other on-farm data for discriminating cows of good versus poor likelihood of conception to first insemination (i.e., pregnant vs. open).
31495611	1	130	link	MIR-derived	180:190	arg1	traits					192:197	MIR-derived traits	180:197	MIR-derived traits including milk composition, milk fatty acids, and blood metabolic profiles (fatty acids, β-hydroxybutyrate, and urea)	180:315	The objective of this study was to investigate the potential of milk mid-infrared (MIR) spectroscopy, MIR-derived traits including milk composition, milk fatty acids, and blood metabolic profiles (fatty acids, β-hydroxybutyrate, and urea), and other on-farm data for discriminating cows of good versus poor likelihood of conception to first insemination (i.e., pregnant vs. open).
31495611	1	130	link	MIR-derived	180:190	arg1	composition					214:224	milk composition	209:224	milk composition	209:224	The objective of this study was to investigate the potential of milk mid-infrared (MIR) spectroscopy, MIR-derived traits including milk composition, milk fatty acids, and blood metabolic profiles (fatty acids, β-hydroxybutyrate, and urea), and other on-farm data for discriminating cows of good versus poor likelihood of conception to first insemination (i.e., pregnant vs. open).
31495611	2	131	theme	Australian	567:576	arg1	states					578:583	3 Australian states	565:583	3 Australian states	565:583	A total of 6,488 spectral and milk production records of 2,987 cows from 19 commercial dairy herds across 3 Australian states were used.
31495611	4	132	theme	milk	686:689	arg1	production					691:700	milk production	686:700	milk production	686:700	Model 1 included milk production; concentrations of fat, protein, and lactose; somatic cell count; age at calving; days in milk at herd test; and days from calving to insemination.
31495611	6	133	theme	MIR	960:962	arg1	spectrum					964:971	The MIR spectrum	956:971	The MIR spectrum collected before first insemination	956:1007	The MIR spectrum collected before first insemination was added to model 2 to form model 3.
31495611	16	134	theme	0.72	2686:2689	arg1	sensitivity					2626:2636	sensitivity	2626:2636	sensitivity	2626:2636	Incorporating MIR spectra into model 2 resulted in sensitivity, specificity, and AUC values of 0.73, 0.63, and 0.72, respectively (model 3).
31495611	16	134	theme	0.72	2686:2689	arg1	values					2660:2665	AUC values	2656:2665	AUC values of 0.73, 0.63, and 0.72	2656:2689	Incorporating MIR spectra into model 2 resulted in sensitivity, specificity, and AUC values of 0.73, 0.63, and 0.72, respectively (model 3).
31495611	16	134	theme	0.72	2686:2689	arg1	specificity					2639:2649	specificity	2639:2649	specificity	2639:2649	Incorporating MIR spectra into model 2 resulted in sensitivity, specificity, and AUC values of 0.73, 0.63, and 0.72, respectively (model 3).
31495611	4	135	from	count	761:765	arg1	milk					792:795	milk	792:795	milk at herd test	792:808	Model 1 included milk production; concentrations of fat, protein, and lactose; somatic cell count; age at calving; days in milk at herd test; and days from calving to insemination.
31495611	4	135	from	count	761:765	arg1	test					805:808	herd test	800:808	herd test	800:808	Model 1 included milk production; concentrations of fat, protein, and lactose; somatic cell count; age at calving; days in milk at herd test; and days from calving to insemination.
31495611	18	136	theme	0.75	3046:3049	arg1	values					3036:3041	AUC values	3032:3041	AUC values of 0.75, 0.66, and 0.75, respectively	3032:3079	Adding the fertility genomic estimated breeding value and animal genotypes (model 7) produced the highest prediction accuracy, with sensitivity, specificity, and AUC values of 0.75, 0.66, and 0.75, respectively.
31495611	18	136	theme	0.75	3046:3049	arg1	specificity					3015:3025	specificity	3015:3025	specificity	3015:3025	Adding the fertility genomic estimated breeding value and animal genotypes (model 7) produced the highest prediction accuracy, with sensitivity, specificity, and AUC values of 0.75, 0.66, and 0.75, respectively.
31495611	18	136	theme	0.75	3046:3049	arg1	sensitivity					3002:3012	sensitivity	3002:3012	sensitivity	3002:3012	Adding the fertility genomic estimated breeding value and animal genotypes (model 7) produced the highest prediction accuracy, with sensitivity, specificity, and AUC values of 0.75, 0.66, and 0.75, respectively.
31495611	13	137	from	difference	2117:2126	arg1	AUC					2131:2133	AUC	2131:2133	AUC ranging between 0.01 and 0.09	2131:2163	The results showed that in all models, prediction accuracy obtained through 10-fold random cross-validation was higher than that of herd-by-herd external validation, with the difference in AUC ranging between 0.01 and 0.09.
31495611	1	138	theme	on-farm	328:334	arg1	profiles					265:272	blood metabolic profiles	249:272	blood metabolic profiles (fatty acids, β-hydroxybutyrate, and urea)	249:315	The objective of this study was to investigate the potential of milk mid-infrared (MIR) spectroscopy, MIR-derived traits including milk composition, milk fatty acids, and blood metabolic profiles (fatty acids, β-hydroxybutyrate, and urea), and other on-farm data for discriminating cows of good versus poor likelihood of conception to first insemination (i.e., pregnant vs. open).
31495611	1	138	theme	on-farm	328:334	arg1	acids					238:242	milk fatty acids	227:242	milk fatty acids	227:242	The objective of this study was to investigate the potential of milk mid-infrared (MIR) spectroscopy, MIR-derived traits including milk composition, milk fatty acids, and blood metabolic profiles (fatty acids, β-hydroxybutyrate, and urea), and other on-farm data for discriminating cows of good versus poor likelihood of conception to first insemination (i.e., pregnant vs. open).
31495611	1	138	theme	on-farm	328:334	arg1	data					336:339	other on-farm data	322:339	other on-farm data for discriminating cows of good versus poor likelihood of conception to first insemination (i.e., pregnant vs. open)	322:456	The objective of this study was to investigate the potential of milk mid-infrared (MIR) spectroscopy, MIR-derived traits including milk composition, milk fatty acids, and blood metabolic profiles (fatty acids, β-hydroxybutyrate, and urea), and other on-farm data for discriminating cows of good versus poor likelihood of conception to first insemination (i.e., pregnant vs. open).
31495611	1	138	theme	on-farm	328:334	arg1	composition					214:224	milk composition	209:224	milk composition	209:224	The objective of this study was to investigate the potential of milk mid-infrared (MIR) spectroscopy, MIR-derived traits including milk composition, milk fatty acids, and blood metabolic profiles (fatty acids, β-hydroxybutyrate, and urea), and other on-farm data for discriminating cows of good versus poor likelihood of conception to first insemination (i.e., pregnant vs. open).
31495611	1	139	dep	pregnant	439:446	arg1	i.e.					433:436	i.e.	433:436	i.e.	433:436	The objective of this study was to investigate the potential of milk mid-infrared (MIR) spectroscopy, MIR-derived traits including milk composition, milk fatty acids, and blood metabolic profiles (fatty acids, β-hydroxybutyrate, and urea), and other on-farm data for discriminating cows of good versus poor likelihood of conception to first insemination (i.e., pregnant vs. open).
31495611	18	140	theme	fertility	2881:2889	arg1	value					2918:2922	estimated breeding value	2899:2922	estimated breeding value	2899:2922	Adding the fertility genomic estimated breeding value and animal genotypes (model 7) produced the highest prediction accuracy, with sensitivity, specificity, and AUC values of 0.75, 0.66, and 0.75, respectively.
31495611	1	141	theme	metabolic	255:263	arg1	profiles					265:272	blood metabolic profiles	249:272	blood metabolic profiles (fatty acids, β-hydroxybutyrate, and urea)	249:315	The objective of this study was to investigate the potential of milk mid-infrared (MIR) spectroscopy, MIR-derived traits including milk composition, milk fatty acids, and blood metabolic profiles (fatty acids, β-hydroxybutyrate, and urea), and other on-farm data for discriminating cows of good versus poor likelihood of conception to first insemination (i.e., pregnant vs. open).
31495611	7	142	theme	metabolic	1114:1122	arg1	profiles					1124:1131	blood metabolic profiles	1108:1131	blood metabolic profiles	1108:1131	Fat, protein, and lactose percentages, milk fatty acids, and blood metabolic profiles were removed from model 3 to create model 4.
31495611	18	143	theme	prediction	2976:2985	arg1	accuracy					2987:2994	the highest prediction accuracy	2964:2994	the highest prediction accuracy	2964:2994	Adding the fertility genomic estimated breeding value and animal genotypes (model 7) produced the highest prediction accuracy, with sensitivity, specificity, and AUC values of 0.75, 0.66, and 0.75, respectively.
31495611	17	144	theme	comparable	2720:2729	arg1	accuracies					2742:2751	The comparable prediction accuracies	2716:2751	The comparable prediction accuracies	2716:2751	The comparable prediction accuracies observed for models 3 and 4 mean that useful information from MIR-derived traits is already included in the spectra.
31495611	16	145	dep	respectively	2692:2703	arg1	model					2706:2710	model 3	2706:2712	model 3	2706:2712	Incorporating MIR spectra into model 2 resulted in sensitivity, specificity, and AUC values of 0.73, 0.63, and 0.72, respectively (model 3).
31495611	19	146	theme	0.01	3237:3240	arg1	accuracy					3220:3227	the prediction accuracy	3205:3227	the prediction accuracy of only 0.01 and 0.02	3205:3249	However, removing either the fertility estimated breeding value or animal genotype from model 7 resulted in a reduction of the prediction accuracy of only 0.01 and 0.02, respectively.
31495611	0	147	theme	cows	35:38	arg1	fertility					16:24	the fertility	12:24	the fertility of dairy cows using milk mid-infrared spectroscopy	12:75	Classifying the fertility of dairy cows using milk mid-infrared spectroscopy.
31495611	12	148	theme	accuracy	1690:1697	arg1	sensitivity					1713:1723	sensitivity	1713:1723	sensitivity (i.e., the proportion of pregnant cows that were correctly classified)	1713:1794	The accuracy measures were sensitivity (i.e., the proportion of pregnant cows that were correctly classified), specificity (i.e., the proportion of open cows that were correctly classified), and area under the curve (AUC) for the receiver operating curve.
31495611	12	148	theme	accuracy	1690:1697	arg1	measures					1699:1706	The accuracy measures	1686:1706	The accuracy measures	1686:1706	The accuracy measures were sensitivity (i.e., the proportion of pregnant cows that were correctly classified), specificity (i.e., the proportion of open cows that were correctly classified), and area under the curve (AUC) for the receiver operating curve.
31495611	14	149	theme	poor-fertility	2290:2303	arg1	cows					2305:2308	good- and poor-fertility cows	2280:2308	good- and poor-fertility cows	2280:2308	In the herd-by-herd external validation, using basic on-farm information (model 1) was not sufficient to classify good- and poor-fertility cows; the sensitivity, specificity, and AUC were around 0.66.
31495611	20	150	with	cows	3360:3363	arg1	accuracy					3422:3429	promising accuracy	3412:3429	promising accuracy	3412:3429	In conclusion, this study indicates that MIR and other on-farm data could be used to classify cows of good and poor likelihood of conception with promising accuracy.
31495611	0	151	theme	milk	46:49	arg1	spectroscopy					64:75	milk mid-infrared spectroscopy	46:75	milk mid-infrared spectroscopy	46:75	Classifying the fertility of dairy cows using milk mid-infrared spectroscopy.
31495611	14	152	theme	good-	2280:2284	arg1	cows					2305:2308	good- and poor-fertility cows	2280:2308	good- and poor-fertility cows	2280:2308	In the herd-by-herd external validation, using basic on-farm information (model 1) was not sufficient to classify good- and poor-fertility cows; the sensitivity, specificity, and AUC were around 0.66.
31495611	12	153	dep	proportion	1820:1829	arg1	i.e.					1810:1813	i.e.	1810:1813	i.e.	1810:1813	The accuracy measures were sensitivity (i.e., the proportion of pregnant cows that were correctly classified), specificity (i.e., the proportion of open cows that were correctly classified), and area under the curve (AUC) for the receiver operating curve.
31495611	4	154	theme	lactose	739:745	arg1	count					761:765	somatic cell count	748:765	somatic cell count	748:765	Model 1 included milk production; concentrations of fat, protein, and lactose; somatic cell count; age at calving; days in milk at herd test; and days from calving to insemination.
31495611	4	154	theme	lactose	739:745	arg1	days					784:787	days	784:787	days in milk at herd test	784:808	Model 1 included milk production; concentrations of fat, protein, and lactose; somatic cell count; age at calving; days in milk at herd test; and days from calving to insemination.
31495611	4	154	theme	lactose	739:745	arg1	days					815:818	days	815:818	days from calving to insemination	815:847	Model 1 included milk production; concentrations of fat, protein, and lactose; somatic cell count; age at calving; days in milk at herd test; and days from calving to insemination.
31495611	4	154	theme	lactose	739:745	arg1	age					768:770	age	768:770	age at calving	768:781	Model 1 included milk production; concentrations of fat, protein, and lactose; somatic cell count; age at calving; days in milk at herd test; and days from calving to insemination.
31495611	4	154	theme	lactose	739:745	arg1	concentrations					703:716	concentrations	703:716	concentrations of fat, protein, and lactose	703:745	Model 1 included milk production; concentrations of fat, protein, and lactose; somatic cell count; age at calving; days in milk at herd test; and days from calving to insemination.
31495611	11	155	theme	external	1665:1672	arg1	ways					1610:1613	2 ways	1608:1613	2 ways: 10-fold random cross-validation and herd-by-herd external validation	1608:1683	The performance of each model was evaluated in 2 ways: 10-fold random cross-validation and herd-by-herd external validation.
31495611	11	155	theme	external	1665:1672	arg1	validation					1674:1683	herd-by-herd external validation	1652:1683	herd-by-herd external validation	1652:1683	The performance of each model was evaluated in 2 ways: 10-fold random cross-validation and herd-by-herd external validation.
31495611	7	156	theme	milk	1086:1089	arg1	acids					1097:1101	milk fatty acids	1086:1101	milk fatty acids	1086:1101	Fat, protein, and lactose percentages, milk fatty acids, and blood metabolic profiles were removed from model 3 to create model 4.
31495611	7	157	attach	removed	1138:1144	arg2	acids					1097:1101	milk fatty acids	1086:1101	milk fatty acids	1086:1101	Fat, protein, and lactose percentages, milk fatty acids, and blood metabolic profiles were removed from model 3 to create model 4.
31495611	7	157	attach	removed	1138:1144	arg2	percentages					1073:1083	Fat, protein, and lactose percentages	1047:1083	Fat, protein, and lactose percentages	1047:1083	Fat, protein, and lactose percentages, milk fatty acids, and blood metabolic profiles were removed from model 3 to create model 4.
31495611	7	157	attach	removed	1138:1144	arg2	profiles					1124:1131	blood metabolic profiles	1108:1131	blood metabolic profiles	1108:1131	Fat, protein, and lactose percentages, milk fatty acids, and blood metabolic profiles were removed from model 3 to create model 4.
31495611	7	157	attach	removed	1138:1144	arg1	model					1151:1155	model 3	1151:1157	model 3	1151:1157	Fat, protein, and lactose percentages, milk fatty acids, and blood metabolic profiles were removed from model 3 to create model 4.
31495611	14	158	theme	herd-by-herd	2173:2184	arg1	validation					2195:2204	the herd-by-herd external validation	2169:2204	the herd-by-herd external validation	2169:2204	In the herd-by-herd external validation, using basic on-farm information (model 1) was not sufficient to classify good- and poor-fertility cows; the sensitivity, specificity, and AUC were around 0.66.
31495611	9	159	theme	information	1432:1442	arg1	sources					1421:1427	all previously described sources	1396:1427	all previously described sources of information	1396:1442	In model 7, all previously described sources of information, but not MIR-derived traits, were used.
31495611	3	160	theme	different	621:629	arg1	variables					643:651	different explanatory variables	621:651	different explanatory variables	621:651	Seven models, comprising different explanatory variables, were examined.
31495611	10	161	theme	least	1524:1528	arg1	analysis					1551:1558	partial least squares discriminant analysis	1516:1558	partial least squares discriminant analysis	1516:1558	The models were developed using partial least squares discriminant analysis.
31495611	1	162	theme	milk	227:230	arg1	acids					238:242	milk fatty acids	227:242	milk fatty acids	227:242	The objective of this study was to investigate the potential of milk mid-infrared (MIR) spectroscopy, MIR-derived traits including milk composition, milk fatty acids, and blood metabolic profiles (fatty acids, β-hydroxybutyrate, and urea), and other on-farm data for discriminating cows of good versus poor likelihood of conception to first insemination (i.e., pregnant vs. open).
31056282	4	0	theme	membrane	707:714	arg1	morphologies					716:727	membrane morphologies	707:727	membrane morphologies	707:727	A polymer brush model of the glycocalyx successfully predicts the effects of polymer size and cell-surface density on membrane morphologies.
31056282	6	1	theme	fundamental	955:965	arg1	role					967:970	a fundamental role	953:970	a fundamental role for the glycocalyx in regulating curved membrane features that serve in communication between cells and with the extracellular matrix	953:1104	Together, our results suggest a fundamental role for the glycocalyx in regulating curved membrane features that serve in communication between cells and with the extracellular matrix.
31056282	1	2	theme	local	148:152	arg1	environment					154:164	the local environment	144:164	the local environment	144:164	Cells bend their plasma membranes into highly curved forms to interact with the local environment, but how shape generation is regulated is not fully resolved.
31056282	2	3	theme	external	318:325	arg1	organization					327:338	external organization	318:338	external organization	318:338	Here, we report a synergy between shape-generating processes in the cell interior and the external organization and composition of the cell-surface glycocalyx.
31056282	3	4	theme	finger-like	543:553	arg1	extensions					555:564	spherical and finger-like extensions	529:564	spherical and finger-like extensions	529:564	Mucin biopolymers and long-chain polysaccharides within the glycocalyx can generate entropic forces that favor or disfavor the projection of spherical and finger-like extensions from the cell surface.
31056282	4	5	theme	brush	599:603	arg1	model					605:609	A polymer brush model	589:609	A polymer brush model of the glycocalyx	589:627	A polymer brush model of the glycocalyx successfully predicts the effects of polymer size and cell-surface density on membrane morphologies.
31056282	3	6	theme	extensions	555:564	arg1	projection					515:524	the projection	511:524	the projection of spherical and finger-like extensions from the cell surface	511:586	Mucin biopolymers and long-chain polysaccharides within the glycocalyx can generate entropic forces that favor or disfavor the projection of spherical and finger-like extensions from the cell surface.
31056282	4	7	theme	polymer	591:597	arg1	model					605:609	A polymer brush model	589:609	A polymer brush model of the glycocalyx	589:627	A polymer brush model of the glycocalyx successfully predicts the effects of polymer size and cell-surface density on membrane morphologies.
31056282	5	8	theme	vesicles	913:920	arg1	secretion					886:894	secretion	886:894	secretion of extracellular vesicles	886:920	Specific glycocalyx compositions can also induce plasma membrane instabilities to generate more exotic undulating and pearled membrane structures and drive secretion of extracellular vesicles.
31056282	3	9	theme	spherical	529:537	arg1	extensions					555:564	spherical and finger-like extensions	529:564	spherical and finger-like extensions	529:564	Mucin biopolymers and long-chain polysaccharides within the glycocalyx can generate entropic forces that favor or disfavor the projection of spherical and finger-like extensions from the cell surface.
31056282	2	10	theme	glycocalyx	376:385	arg1	synergy					246:252	a synergy	244:252	a synergy between shape-generating processes in the cell interior	244:308	Here, we report a synergy between shape-generating processes in the cell interior and the external organization and composition of the cell-surface glycocalyx.
31056282	2	10	theme	glycocalyx	376:385	arg1	organization					327:338	external organization	318:338	external organization	318:338	Here, we report a synergy between shape-generating processes in the cell interior and the external organization and composition of the cell-surface glycocalyx.
31056282	2	10	theme	glycocalyx	376:385	arg1	composition					344:354	composition	344:354	composition	344:354	Here, we report a synergy between shape-generating processes in the cell interior and the external organization and composition of the cell-surface glycocalyx.
31056282	5	11	theme	undulating	833:842	arg1	structures					865:874	more exotic undulating and pearled membrane structures	821:874	more exotic undulating and pearled membrane structures	821:874	Specific glycocalyx compositions can also induce plasma membrane instabilities to generate more exotic undulating and pearled membrane structures and drive secretion of extracellular vesicles.
31056282	5	12	theme	pearled	848:854	arg1	structures					865:874	more exotic undulating and pearled membrane structures	821:874	more exotic undulating and pearled membrane structures	821:874	Specific glycocalyx compositions can also induce plasma membrane instabilities to generate more exotic undulating and pearled membrane structures and drive secretion of extracellular vesicles.
31056282	2	13	theme	cell-surface	363:374	arg1	glycocalyx					376:385	the cell-surface glycocalyx	359:385	the cell-surface glycocalyx	359:385	Here, we report a synergy between shape-generating processes in the cell interior and the external organization and composition of the cell-surface glycocalyx.
31056282	3	14	theme	cell	575:578	arg1	surface					580:586	the cell surface	571:586	the cell surface	571:586	Mucin biopolymers and long-chain polysaccharides within the glycocalyx can generate entropic forces that favor or disfavor the projection of spherical and finger-like extensions from the cell surface.
31056282	3	15	theme	long-chain	410:419	arg1	polysaccharides					421:435	long-chain polysaccharides	410:435	long-chain polysaccharides	410:435	Mucin biopolymers and long-chain polysaccharides within the glycocalyx can generate entropic forces that favor or disfavor the projection of spherical and finger-like extensions from the cell surface.
31056282	0	16	theme	Physical	0:7	arg1	Principles					9:18	Physical Principles	0:18	Physical Principles of Membrane	0:30	Physical Principles of Membrane Shape Regulation by the Glycocalyx.
31056282	5	17	theme	extracellular	899:911	arg1	vesicles					913:920	extracellular vesicles	899:920	extracellular vesicles	899:920	Specific glycocalyx compositions can also induce plasma membrane instabilities to generate more exotic undulating and pearled membrane structures and drive secretion of extracellular vesicles.
31056282	1	18	theme	plasma	85:90	arg1	membranes					92:100	their plasma membranes	79:100	their plasma membranes	79:100	Cells bend their plasma membranes into highly curved forms to interact with the local environment, but how shape generation is regulated is not fully resolved.
31056282	4	19	from	effects	655:661	arg1	morphologies					716:727	membrane morphologies	707:727	membrane morphologies	707:727	A polymer brush model of the glycocalyx successfully predicts the effects of polymer size and cell-surface density on membrane morphologies.
31056282	4	20	theme	size	674:677	arg1	effects					655:661	the effects	651:661	the effects of polymer size and cell-surface density on membrane morphologies	651:727	A polymer brush model of the glycocalyx successfully predicts the effects of polymer size and cell-surface density on membrane morphologies.
31056282	0	21	theme	Membrane	23:30	arg1	Principles					9:18	Physical Principles	0:18	Physical Principles of Membrane	0:30	Physical Principles of Membrane Shape Regulation by the Glycocalyx.
31056282	1	22	theme	shape	175:179	arg1	generation					181:190	shape generation	175:190	shape generation	175:190	Cells bend their plasma membranes into highly curved forms to interact with the local environment, but how shape generation is regulated is not fully resolved.
31056282	6	23	theme	membrane	1012:1019	arg1	features					1021:1028	curved membrane features	1005:1028	curved membrane features that serve in communication between cells and with the extracellular matrix	1005:1104	Together, our results suggest a fundamental role for the glycocalyx in regulating curved membrane features that serve in communication between cells and with the extracellular matrix.
31056282	2	24	theme	shape-generating	262:277	arg1	processes					279:287	shape-generating processes	262:287	shape-generating processes in the cell interior	262:308	Here, we report a synergy between shape-generating processes in the cell interior and the external organization and composition of the cell-surface glycocalyx.
31056282	3	25	theme	entropic	472:479	arg1	forces					481:486	entropic forces	472:486	entropic forces that favor or disfavor the projection of spherical and finger-like extensions from the cell surface	472:586	Mucin biopolymers and long-chain polysaccharides within the glycocalyx can generate entropic forces that favor or disfavor the projection of spherical and finger-like extensions from the cell surface.
31056282	6	26	theme	curved	1005:1010	arg1	features					1021:1028	curved membrane features	1005:1028	curved membrane features that serve in communication between cells and with the extracellular matrix	1005:1104	Together, our results suggest a fundamental role for the glycocalyx in regulating curved membrane features that serve in communication between cells and with the extracellular matrix.
31056282	5	27	theme	Specific	730:737	arg1	compositions					750:761	Specific glycocalyx compositions	730:761	Specific glycocalyx compositions	730:761	Specific glycocalyx compositions can also induce plasma membrane instabilities to generate more exotic undulating and pearled membrane structures and drive secretion of extracellular vesicles.
31056282	4	28	theme	polymer	666:672	arg1	size					674:677	polymer size	666:677	polymer size	666:677	A polymer brush model of the glycocalyx successfully predicts the effects of polymer size and cell-surface density on membrane morphologies.
31056282	5	29	theme	glycocalyx	739:748	arg1	compositions					750:761	Specific glycocalyx compositions	730:761	Specific glycocalyx compositions	730:761	Specific glycocalyx compositions can also induce plasma membrane instabilities to generate more exotic undulating and pearled membrane structures and drive secretion of extracellular vesicles.
31056282	5	30	theme	membrane	856:863	arg1	structures					865:874	more exotic undulating and pearled membrane structures	821:874	more exotic undulating and pearled membrane structures	821:874	Specific glycocalyx compositions can also induce plasma membrane instabilities to generate more exotic undulating and pearled membrane structures and drive secretion of extracellular vesicles.
31056282	6	31	theme	extracellular	1085:1097	arg1	matrix					1099:1104	the extracellular matrix	1081:1104	the extracellular matrix	1081:1104	Together, our results suggest a fundamental role for the glycocalyx in regulating curved membrane features that serve in communication between cells and with the extracellular matrix.
31056282	5	32	theme	exotic	826:831	arg1	structures					865:874	more exotic undulating and pearled membrane structures	821:874	more exotic undulating and pearled membrane structures	821:874	Specific glycocalyx compositions can also induce plasma membrane instabilities to generate more exotic undulating and pearled membrane structures and drive secretion of extracellular vesicles.
31056282	3	33	theme	Mucin	388:392	arg1	biopolymers					394:404	Mucin biopolymers	388:404	Mucin biopolymers	388:404	Mucin biopolymers and long-chain polysaccharides within the glycocalyx can generate entropic forces that favor or disfavor the projection of spherical and finger-like extensions from the cell surface.
31056282	1	34	theme	curved	114:119	arg1	forms					121:125	highly curved forms	107:125	highly curved forms	107:125	Cells bend their plasma membranes into highly curved forms to interact with the local environment, but how shape generation is regulated is not fully resolved.
31056282	2	35	theme	cell	296:299	arg1	interior					301:308	the cell interior	292:308	the cell interior	292:308	Here, we report a synergy between shape-generating processes in the cell interior and the external organization and composition of the cell-surface glycocalyx.
31056282	5	36	theme	plasma	779:784	arg1	instabilities					795:807	plasma membrane instabilities	779:807	plasma membrane instabilities	779:807	Specific glycocalyx compositions can also induce plasma membrane instabilities to generate more exotic undulating and pearled membrane structures and drive secretion of extracellular vesicles.
31056282	4	37	theme	density	696:702	arg1	effects					655:661	the effects	651:661	the effects of polymer size and cell-surface density on membrane morphologies	651:727	A polymer brush model of the glycocalyx successfully predicts the effects of polymer size and cell-surface density on membrane morphologies.
31056282	3	38	from	surface	580:586	arg1	projection					515:524	the projection	511:524	the projection of spherical and finger-like extensions from the cell surface	511:586	Mucin biopolymers and long-chain polysaccharides within the glycocalyx can generate entropic forces that favor or disfavor the projection of spherical and finger-like extensions from the cell surface.
31056282	4	39	theme	glycocalyx	618:627	arg1	model					605:609	A polymer brush model	589:609	A polymer brush model of the glycocalyx	589:627	A polymer brush model of the glycocalyx successfully predicts the effects of polymer size and cell-surface density on membrane morphologies.
31056282	5	40	theme	membrane	786:793	arg1	instabilities					795:807	plasma membrane instabilities	779:807	plasma membrane instabilities	779:807	Specific glycocalyx compositions can also induce plasma membrane instabilities to generate more exotic undulating and pearled membrane structures and drive secretion of extracellular vesicles.
31056282	4	41	theme	cell-surface	683:694	arg1	density					696:702	cell-surface density	683:702	cell-surface density	683:702	A polymer brush model of the glycocalyx successfully predicts the effects of polymer size and cell-surface density on membrane morphologies.
31056282	2	42	from	processes	279:287	arg1	interior					301:308	the cell interior	292:308	the cell interior	292:308	Here, we report a synergy between shape-generating processes in the cell interior and the external organization and composition of the cell-surface glycocalyx.
32036884	2	0	theme	honey	505:509	arg1	bee					501:503	Stingless bee honey	491:509	Stingless bee honey	491:509	Stingless bee honey did not show to be cytotoxic at the tested concentrations (1-100 µM) and also reduced nitric oxide and the secretion of the pro-inflammatory cytokine in the inflamed macrophages.
32036884	1	1	theme	lipopolysaccharides-stimulated	322:351	arg1	macrophages					362:372	lipopolysaccharides-stimulated RAW264.7 macrophages	322:372	lipopolysaccharides-stimulated RAW264.7 macrophages	322:372	The objective of this study was to investigate and quantify the composition phenolic, reducing capacity, the free radical scavenging activity, as well as, the anti-inflammatory effect evaluated against lipopolysaccharides-stimulated RAW264.7 macrophages through modulation of inflammatory mediators, in eight stingless bee honey types (Meliponinae) from southern Brazil.
32036884	1	2	theme	radical	234:240	arg1	activity					253:260	the free radical scavenging activity	225:260	the free radical scavenging activity	225:260	The objective of this study was to investigate and quantify the composition phenolic, reducing capacity, the free radical scavenging activity, as well as, the anti-inflammatory effect evaluated against lipopolysaccharides-stimulated RAW264.7 macrophages through modulation of inflammatory mediators, in eight stingless bee honey types (Meliponinae) from southern Brazil.
32036884	1	2	theme	radical	234:240	arg1	composition					184:194	the composition phenolic	180:203	the composition phenolic	180:203	The objective of this study was to investigate and quantify the composition phenolic, reducing capacity, the free radical scavenging activity, as well as, the anti-inflammatory effect evaluated against lipopolysaccharides-stimulated RAW264.7 macrophages through modulation of inflammatory mediators, in eight stingless bee honey types (Meliponinae) from southern Brazil.
32036884	0	3	theme	stingless	85:93	arg1	bee					95:97	stingless bee honey	85:103	stingless bee honey (Meliponinae)	85:117	Investigation of phenolic compounds, antioxidant and anti-inflammatory activities in stingless bee honey (Meliponinae).
32036884	0	3	theme	stingless	85:93	arg1	Meliponinae					106:116	Meliponinae	106:116	Meliponinae	106:116	Investigation of phenolic compounds, antioxidant and anti-inflammatory activities in stingless bee honey (Meliponinae).
32036884	1	4	theme	RAW264.7	353:360	arg1	macrophages					362:372	lipopolysaccharides-stimulated RAW264.7 macrophages	322:372	lipopolysaccharides-stimulated RAW264.7 macrophages	322:372	The objective of this study was to investigate and quantify the composition phenolic, reducing capacity, the free radical scavenging activity, as well as, the anti-inflammatory effect evaluated against lipopolysaccharides-stimulated RAW264.7 macrophages through modulation of inflammatory mediators, in eight stingless bee honey types (Meliponinae) from southern Brazil.
32036884	2	5	theme	nitric	597:602	arg1	oxide					604:608	nitric oxide	597:608	nitric oxide	597:608	Stingless bee honey did not show to be cytotoxic at the tested concentrations (1-100 µM) and also reduced nitric oxide and the secretion of the pro-inflammatory cytokine in the inflamed macrophages.
32036884	4	6	dep	provide	1026:1032	arg1	could					1014:1018	could	1014:1018	could	1014:1018	All these findings indicate that stingless bee honey could be an important source of natural compounds presenting anti-inflammatory and antioxidant effect, which could would provide health benefits when included in the diet.
32036884	1	7	theme	scavenging	242:251	arg1	activity					253:260	the free radical scavenging activity	225:260	the free radical scavenging activity	225:260	The objective of this study was to investigate and quantify the composition phenolic, reducing capacity, the free radical scavenging activity, as well as, the anti-inflammatory effect evaluated against lipopolysaccharides-stimulated RAW264.7 macrophages through modulation of inflammatory mediators, in eight stingless bee honey types (Meliponinae) from southern Brazil.
32036884	1	7	theme	scavenging	242:251	arg1	composition					184:194	the composition phenolic	180:203	the composition phenolic	180:203	The objective of this study was to investigate and quantify the composition phenolic, reducing capacity, the free radical scavenging activity, as well as, the anti-inflammatory effect evaluated against lipopolysaccharides-stimulated RAW264.7 macrophages through modulation of inflammatory mediators, in eight stingless bee honey types (Meliponinae) from southern Brazil.
32036884	3	8	theme	honey	694:698	arg1	samples					700:706	Two honey samples	690:706	Two honey samples	690:706	Two honey samples showed the ability to increase the secretion of anti-inflammatory cytokine (interleukin-10), suggesting a significant anti-inflammatory effect.
32036884	2	9	theme	cytokine	652:659	arg1	oxide					604:608	nitric oxide	597:608	nitric oxide	597:608	Stingless bee honey did not show to be cytotoxic at the tested concentrations (1-100 µM) and also reduced nitric oxide and the secretion of the pro-inflammatory cytokine in the inflamed macrophages.
32036884	2	9	theme	cytokine	652:659	arg1	secretion					618:626	the secretion	614:626	the secretion of the pro-inflammatory cytokine in the inflamed macrophages	614:687	Stingless bee honey did not show to be cytotoxic at the tested concentrations (1-100 µM) and also reduced nitric oxide and the secretion of the pro-inflammatory cytokine in the inflamed macrophages.
32036884	3	10	theme	anti-inflammatory	826:842	arg1	effect					844:849	a significant anti-inflammatory effect	812:849	a significant anti-inflammatory effect	812:849	Two honey samples showed the ability to increase the secretion of anti-inflammatory cytokine (interleukin-10), suggesting a significant anti-inflammatory effect.
32036884	0	11	theme	honey	99:103	arg1	bee					95:97	stingless bee honey	85:103	stingless bee honey (Meliponinae)	85:117	Investigation of phenolic compounds, antioxidant and anti-inflammatory activities in stingless bee honey (Meliponinae).
32036884	0	11	theme	honey	99:103	arg1	Meliponinae					106:116	Meliponinae	106:116	Meliponinae	106:116	Investigation of phenolic compounds, antioxidant and anti-inflammatory activities in stingless bee honey (Meliponinae).
32036884	4	12	theme	honey	899:903	arg1	bee					895:897	stingless bee honey	885:903	stingless bee honey	885:903	All these findings indicate that stingless bee honey could be an important source of natural compounds presenting anti-inflammatory and antioxidant effect, which could would provide health benefits when included in the diet.
32036884	4	12	theme	honey	899:903	arg1	source					927:932	an important source	914:932	an important source	914:932	All these findings indicate that stingless bee honey could be an important source of natural compounds presenting anti-inflammatory and antioxidant effect, which could would provide health benefits when included in the diet.
32036884	2	13	theme	pro-inflammatory	635:650	arg1	cytokine					652:659	the pro-inflammatory cytokine	631:659	the pro-inflammatory cytokine	631:659	Stingless bee honey did not show to be cytotoxic at the tested concentrations (1-100 µM) and also reduced nitric oxide and the secretion of the pro-inflammatory cytokine in the inflamed macrophages.
32036884	4	14	theme	antioxidant	988:998	arg1	effect					1000:1005	anti-inflammatory and antioxidant effect	966:1005	anti-inflammatory and antioxidant effect	966:1005	All these findings indicate that stingless bee honey could be an important source of natural compounds presenting anti-inflammatory and antioxidant effect, which could would provide health benefits when included in the diet.
32036884	0	15	from	antioxidant	37:47	arg1	bee					95:97	stingless bee honey	85:103	stingless bee honey (Meliponinae)	85:117	Investigation of phenolic compounds, antioxidant and anti-inflammatory activities in stingless bee honey (Meliponinae).
32036884	0	15	from	antioxidant	37:47	arg1	Meliponinae					106:116	Meliponinae	106:116	Meliponinae	106:116	Investigation of phenolic compounds, antioxidant and anti-inflammatory activities in stingless bee honey (Meliponinae).
32036884	4	16	theme	health	1034:1039	arg1	benefits					1041:1048	health benefits	1034:1048	health benefits	1034:1048	All these findings indicate that stingless bee honey could be an important source of natural compounds presenting anti-inflammatory and antioxidant effect, which could would provide health benefits when included in the diet.
32036884	1	17	theme	southern	474:481	arg1	Brazil					483:488	southern Brazil	474:488	southern Brazil	474:488	The objective of this study was to investigate and quantify the composition phenolic, reducing capacity, the free radical scavenging activity, as well as, the anti-inflammatory effect evaluated against lipopolysaccharides-stimulated RAW264.7 macrophages through modulation of inflammatory mediators, in eight stingless bee honey types (Meliponinae) from southern Brazil.
32036884	1	18	theme	inflammatory	396:407	arg1	mediators					409:417	inflammatory mediators	396:417	inflammatory mediators	396:417	The objective of this study was to investigate and quantify the composition phenolic, reducing capacity, the free radical scavenging activity, as well as, the anti-inflammatory effect evaluated against lipopolysaccharides-stimulated RAW264.7 macrophages through modulation of inflammatory mediators, in eight stingless bee honey types (Meliponinae) from southern Brazil.
32036884	2	19	theme	inflamed	668:675	arg1	macrophages					677:687	the inflamed macrophages	664:687	the inflamed macrophages	664:687	Stingless bee honey did not show to be cytotoxic at the tested concentrations (1-100 µM) and also reduced nitric oxide and the secretion of the pro-inflammatory cytokine in the inflamed macrophages.
32036884	4	20	theme	stingless	885:893	arg1	bee					895:897	stingless bee honey	885:903	stingless bee honey	885:903	All these findings indicate that stingless bee honey could be an important source of natural compounds presenting anti-inflammatory and antioxidant effect, which could would provide health benefits when included in the diet.
32036884	4	20	theme	stingless	885:893	arg1	source					927:932	an important source	914:932	an important source	914:932	All these findings indicate that stingless bee honey could be an important source of natural compounds presenting anti-inflammatory and antioxidant effect, which could would provide health benefits when included in the diet.
32036884	0	21	theme	compounds	26:34	arg1	antioxidant					37:47	antioxidant	37:47	antioxidant	37:47	Investigation of phenolic compounds, antioxidant and anti-inflammatory activities in stingless bee honey (Meliponinae).
32036884	0	21	theme	compounds	26:34	arg1	Investigation					0:12	Investigation	0:12	Investigation of phenolic compounds	0:34	Investigation of phenolic compounds, antioxidant and anti-inflammatory activities in stingless bee honey (Meliponinae).
32036884	0	21	theme	compounds	26:34	arg1	activities					71:80	anti-inflammatory activities	53:80	anti-inflammatory activities	53:80	Investigation of phenolic compounds, antioxidant and anti-inflammatory activities in stingless bee honey (Meliponinae).
32036884	3	22	theme	anti-inflammatory	756:772	arg1	interleukin-10					784:797	interleukin-10	784:797	interleukin-10	784:797	Two honey samples showed the ability to increase the secretion of anti-inflammatory cytokine (interleukin-10), suggesting a significant anti-inflammatory effect.
32036884	3	22	theme	anti-inflammatory	756:772	arg1	cytokine					774:781	anti-inflammatory cytokine	756:781	anti-inflammatory cytokine (interleukin-10)	756:798	Two honey samples showed the ability to increase the secretion of anti-inflammatory cytokine (interleukin-10), suggesting a significant anti-inflammatory effect.
32036884	4	23	theme	natural	937:943	arg1	compounds					945:953	natural compounds	937:953	natural compounds presenting anti-inflammatory and antioxidant effect	937:1005	All these findings indicate that stingless bee honey could be an important source of natural compounds presenting anti-inflammatory and antioxidant effect, which could would provide health benefits when included in the diet.
32036884	0	24	theme	phenolic	17:24	arg1	compounds					26:34	phenolic compounds	17:34	phenolic compounds	17:34	Investigation of phenolic compounds, antioxidant and anti-inflammatory activities in stingless bee honey (Meliponinae).
32036884	2	25	from	oxide	604:608	arg1	macrophages					677:687	the inflamed macrophages	664:687	the inflamed macrophages	664:687	Stingless bee honey did not show to be cytotoxic at the tested concentrations (1-100 µM) and also reduced nitric oxide and the secretion of the pro-inflammatory cytokine in the inflamed macrophages.
32036884	2	26	theme	tested	547:552	arg1	1-100 µM					570:577	1-100 µM	570:577	1-100 µM	570:577	Stingless bee honey did not show to be cytotoxic at the tested concentrations (1-100 µM) and also reduced nitric oxide and the secretion of the pro-inflammatory cytokine in the inflamed macrophages.
32036884	2	26	theme	tested	547:552	arg1	concentrations					554:567	the tested concentrations	543:567	the tested concentrations (1-100 µM)	543:578	Stingless bee honey did not show to be cytotoxic at the tested concentrations (1-100 µM) and also reduced nitric oxide and the secretion of the pro-inflammatory cytokine in the inflamed macrophages.
32036884	1	27	theme	mediators	409:417	arg1	modulation					382:391	modulation	382:391	modulation of inflammatory mediators	382:417	The objective of this study was to investigate and quantify the composition phenolic, reducing capacity, the free radical scavenging activity, as well as, the anti-inflammatory effect evaluated against lipopolysaccharides-stimulated RAW264.7 macrophages through modulation of inflammatory mediators, in eight stingless bee honey types (Meliponinae) from southern Brazil.
32036884	1	28	theme	phenolic	196:203	arg1	capacity					215:222	reducing capacity	206:222	reducing capacity	206:222	The objective of this study was to investigate and quantify the composition phenolic, reducing capacity, the free radical scavenging activity, as well as, the anti-inflammatory effect evaluated against lipopolysaccharides-stimulated RAW264.7 macrophages through modulation of inflammatory mediators, in eight stingless bee honey types (Meliponinae) from southern Brazil.
32036884	1	28	theme	phenolic	196:203	arg1	activity					253:260	the free radical scavenging activity	225:260	the free radical scavenging activity	225:260	The objective of this study was to investigate and quantify the composition phenolic, reducing capacity, the free radical scavenging activity, as well as, the anti-inflammatory effect evaluated against lipopolysaccharides-stimulated RAW264.7 macrophages through modulation of inflammatory mediators, in eight stingless bee honey types (Meliponinae) from southern Brazil.
32036884	1	28	theme	phenolic	196:203	arg1	composition					184:194	the composition phenolic	180:203	the composition phenolic	180:203	The objective of this study was to investigate and quantify the composition phenolic, reducing capacity, the free radical scavenging activity, as well as, the anti-inflammatory effect evaluated against lipopolysaccharides-stimulated RAW264.7 macrophages through modulation of inflammatory mediators, in eight stingless bee honey types (Meliponinae) from southern Brazil.
32036884	2	29	theme	Stingless	491:499	arg1	bee					501:503	Stingless bee honey	491:509	Stingless bee honey	491:509	Stingless bee honey did not show to be cytotoxic at the tested concentrations (1-100 µM) and also reduced nitric oxide and the secretion of the pro-inflammatory cytokine in the inflamed macrophages.
32036884	4	30	theme	anti-inflammatory	966:982	arg1	effect					1000:1005	anti-inflammatory and antioxidant effect	966:1005	anti-inflammatory and antioxidant effect	966:1005	All these findings indicate that stingless bee honey could be an important source of natural compounds presenting anti-inflammatory and antioxidant effect, which could would provide health benefits when included in the diet.
32036884	4	31	theme	compounds	945:953	arg1	bee					895:897	stingless bee honey	885:903	stingless bee honey	885:903	All these findings indicate that stingless bee honey could be an important source of natural compounds presenting anti-inflammatory and antioxidant effect, which could would provide health benefits when included in the diet.
32036884	4	31	theme	compounds	945:953	arg1	source					927:932	an important source	914:932	an important source	914:932	All these findings indicate that stingless bee honey could be an important source of natural compounds presenting anti-inflammatory and antioxidant effect, which could would provide health benefits when included in the diet.
32036884	1	32	theme	reducing	206:213	arg1	capacity					215:222	reducing capacity	206:222	reducing capacity	206:222	The objective of this study was to investigate and quantify the composition phenolic, reducing capacity, the free radical scavenging activity, as well as, the anti-inflammatory effect evaluated against lipopolysaccharides-stimulated RAW264.7 macrophages through modulation of inflammatory mediators, in eight stingless bee honey types (Meliponinae) from southern Brazil.
32036884	1	32	theme	reducing	206:213	arg1	composition					184:194	the composition phenolic	180:203	the composition phenolic	180:203	The objective of this study was to investigate and quantify the composition phenolic, reducing capacity, the free radical scavenging activity, as well as, the anti-inflammatory effect evaluated against lipopolysaccharides-stimulated RAW264.7 macrophages through modulation of inflammatory mediators, in eight stingless bee honey types (Meliponinae) from southern Brazil.
32036884	0	33	theme	anti-inflammatory	53:69	arg1	activities					71:80	anti-inflammatory activities	53:80	anti-inflammatory activities	53:80	Investigation of phenolic compounds, antioxidant and anti-inflammatory activities in stingless bee honey (Meliponinae).
32036884	0	33	theme	anti-inflammatory	53:69	arg1	Investigation					0:12	Investigation	0:12	Investigation of phenolic compounds	0:34	Investigation of phenolic compounds, antioxidant and anti-inflammatory activities in stingless bee honey (Meliponinae).
32036884	1	34	theme	anti-inflammatory	279:295	arg1	as					271:272	as	271:272	as	271:272	The objective of this study was to investigate and quantify the composition phenolic, reducing capacity, the free radical scavenging activity, as well as, the anti-inflammatory effect evaluated against lipopolysaccharides-stimulated RAW264.7 macrophages through modulation of inflammatory mediators, in eight stingless bee honey types (Meliponinae) from southern Brazil.
32036884	1	34	theme	anti-inflammatory	279:295	arg1	effect					297:302	the anti-inflammatory effect	275:302	the anti-inflammatory effect evaluated against lipopolysaccharides-stimulated RAW264.7 macrophages through modulation of inflammatory mediators	275:417	The objective of this study was to investigate and quantify the composition phenolic, reducing capacity, the free radical scavenging activity, as well as, the anti-inflammatory effect evaluated against lipopolysaccharides-stimulated RAW264.7 macrophages through modulation of inflammatory mediators, in eight stingless bee honey types (Meliponinae) from southern Brazil.
32036884	0	35	from	activities	71:80	arg1	bee					95:97	stingless bee honey	85:103	stingless bee honey (Meliponinae)	85:117	Investigation of phenolic compounds, antioxidant and anti-inflammatory activities in stingless bee honey (Meliponinae).
32036884	0	35	from	activities	71:80	arg1	Meliponinae					106:116	Meliponinae	106:116	Meliponinae	106:116	Investigation of phenolic compounds, antioxidant and anti-inflammatory activities in stingless bee honey (Meliponinae).
32036884	3	36	theme	cytokine	774:781	arg1	secretion					743:751	the secretion	739:751	the secretion of anti-inflammatory cytokine (interleukin-10)	739:798	Two honey samples showed the ability to increase the secretion of anti-inflammatory cytokine (interleukin-10), suggesting a significant anti-inflammatory effect.
32036884	1	37	theme	stingless	429:437	arg1	Meliponinae					456:466	Meliponinae	456:466	Meliponinae	456:466	The objective of this study was to investigate and quantify the composition phenolic, reducing capacity, the free radical scavenging activity, as well as, the anti-inflammatory effect evaluated against lipopolysaccharides-stimulated RAW264.7 macrophages through modulation of inflammatory mediators, in eight stingless bee honey types (Meliponinae) from southern Brazil.
32036884	1	37	theme	stingless	429:437	arg1	types					449:453	eight stingless bee honey types	423:453	eight stingless bee honey types (Meliponinae) from southern Brazil	423:488	The objective of this study was to investigate and quantify the composition phenolic, reducing capacity, the free radical scavenging activity, as well as, the anti-inflammatory effect evaluated against lipopolysaccharides-stimulated RAW264.7 macrophages through modulation of inflammatory mediators, in eight stingless bee honey types (Meliponinae) from southern Brazil.
32036884	2	38	from	secretion	618:626	arg1	macrophages					677:687	the inflamed macrophages	664:687	the inflamed macrophages	664:687	Stingless bee honey did not show to be cytotoxic at the tested concentrations (1-100 µM) and also reduced nitric oxide and the secretion of the pro-inflammatory cytokine in the inflamed macrophages.
32036884	1	39	theme	bee	439:441	arg1	Meliponinae					456:466	Meliponinae	456:466	Meliponinae	456:466	The objective of this study was to investigate and quantify the composition phenolic, reducing capacity, the free radical scavenging activity, as well as, the anti-inflammatory effect evaluated against lipopolysaccharides-stimulated RAW264.7 macrophages through modulation of inflammatory mediators, in eight stingless bee honey types (Meliponinae) from southern Brazil.
32036884	1	39	theme	bee	439:441	arg1	types					449:453	eight stingless bee honey types	423:453	eight stingless bee honey types (Meliponinae) from southern Brazil	423:488	The objective of this study was to investigate and quantify the composition phenolic, reducing capacity, the free radical scavenging activity, as well as, the anti-inflammatory effect evaluated against lipopolysaccharides-stimulated RAW264.7 macrophages through modulation of inflammatory mediators, in eight stingless bee honey types (Meliponinae) from southern Brazil.
32036884	4	40	theme	important	917:925	arg1	bee					895:897	stingless bee honey	885:903	stingless bee honey	885:903	All these findings indicate that stingless bee honey could be an important source of natural compounds presenting anti-inflammatory and antioxidant effect, which could would provide health benefits when included in the diet.
32036884	4	40	theme	important	917:925	arg1	source					927:932	an important source	914:932	an important source	914:932	All these findings indicate that stingless bee honey could be an important source of natural compounds presenting anti-inflammatory and antioxidant effect, which could would provide health benefits when included in the diet.
32036884	1	41	theme	honey	443:447	arg1	Meliponinae					456:466	Meliponinae	456:466	Meliponinae	456:466	The objective of this study was to investigate and quantify the composition phenolic, reducing capacity, the free radical scavenging activity, as well as, the anti-inflammatory effect evaluated against lipopolysaccharides-stimulated RAW264.7 macrophages through modulation of inflammatory mediators, in eight stingless bee honey types (Meliponinae) from southern Brazil.
32036884	1	41	theme	honey	443:447	arg1	types					449:453	eight stingless bee honey types	423:453	eight stingless bee honey types (Meliponinae) from southern Brazil	423:488	The objective of this study was to investigate and quantify the composition phenolic, reducing capacity, the free radical scavenging activity, as well as, the anti-inflammatory effect evaluated against lipopolysaccharides-stimulated RAW264.7 macrophages through modulation of inflammatory mediators, in eight stingless bee honey types (Meliponinae) from southern Brazil.
32036884	1	42	from	Brazil	483:488	arg1	Meliponinae					456:466	Meliponinae	456:466	Meliponinae	456:466	The objective of this study was to investigate and quantify the composition phenolic, reducing capacity, the free radical scavenging activity, as well as, the anti-inflammatory effect evaluated against lipopolysaccharides-stimulated RAW264.7 macrophages through modulation of inflammatory mediators, in eight stingless bee honey types (Meliponinae) from southern Brazil.
32036884	1	42	from	Brazil	483:488	arg1	types					449:453	eight stingless bee honey types	423:453	eight stingless bee honey types (Meliponinae) from southern Brazil	423:488	The objective of this study was to investigate and quantify the composition phenolic, reducing capacity, the free radical scavenging activity, as well as, the anti-inflammatory effect evaluated against lipopolysaccharides-stimulated RAW264.7 macrophages through modulation of inflammatory mediators, in eight stingless bee honey types (Meliponinae) from southern Brazil.
32036884	3	43	theme	significant	814:824	arg1	effect					844:849	a significant anti-inflammatory effect	812:849	a significant anti-inflammatory effect	812:849	Two honey samples showed the ability to increase the secretion of anti-inflammatory cytokine (interleukin-10), suggesting a significant anti-inflammatory effect.
32036884	2	44	from	concentrations	554:567	arg1	cytotoxic					530:538	cytotoxic	530:538	cytotoxic	530:538	Stingless bee honey did not show to be cytotoxic at the tested concentrations (1-100 µM) and also reduced nitric oxide and the secretion of the pro-inflammatory cytokine in the inflamed macrophages.
32036884	1	45	theme	study	142:146	arg1	objective					124:132	The objective	120:132	The objective of this study	120:146	The objective of this study was to investigate and quantify the composition phenolic, reducing capacity, the free radical scavenging activity, as well as, the anti-inflammatory effect evaluated against lipopolysaccharides-stimulated RAW264.7 macrophages through modulation of inflammatory mediators, in eight stingless bee honey types (Meliponinae) from southern Brazil.
32036884	1	46	theme	free	229:232	arg1	radical					234:240	the free radical	225:240	the free radical scavenging activity	225:260	The objective of this study was to investigate and quantify the composition phenolic, reducing capacity, the free radical scavenging activity, as well as, the anti-inflammatory effect evaluated against lipopolysaccharides-stimulated RAW264.7 macrophages through modulation of inflammatory mediators, in eight stingless bee honey types (Meliponinae) from southern Brazil.
31408199	2	0	theme	Sterile	432:438	arg1	discs					443:447	METHODOLOGY Sterile HA discs	420:447	METHODOLOGY Sterile HA discs	420:447	METHODOLOGY Sterile HA discs were conditioned with collagen, saliva or serum, and inoculated with E. faecalis to form 3-day and 21-day-old biofilms.
31408199	7	1	theme	carbohydrate	1200:1211	arg1	content					1213:1219	the total carbohydrate content	1190:1219	the total carbohydrate content in the 21-day biofilms	1190:1242	Although the biochemical analysis revealed that surface conditioning had no significant effect on the total carbohydrate content in the 21-day biofilms, confocal microscopic analysis revealed that collagen and saliva conditioning selectively increased the polysaccharide content of 21-day biofilms, compared to the 3-day biofilms (P < 0.05).
31408199	2	2	theme	3-day	538:542	arg1	biofilms					559:566	3-day and 21-day-old biofilms	538:566	3-day and 21-day-old biofilms	538:566	METHODOLOGY Sterile HA discs were conditioned with collagen, saliva or serum, and inoculated with E. faecalis to form 3-day and 21-day-old biofilms.
31408199	4	3	theme	culture-dependent	642:658	arg1	methods					723:729	culture-dependent and independent (confocal microscopy and biochemical analysis) methods	642:729	culture-dependent and independent (confocal microscopy and biochemical analysis) methods	642:729	The biofilms were analysed using culture-dependent and independent (confocal microscopy and biochemical analysis) methods, to determine the colony-forming units and the biofilm matrix composition (polysaccharides and proteins), respectively.
31408199	1	4	theme	hydroxyapatite	224:237	arg1	discs					244:248	hydroxyapatite (HA) discs	224:248	hydroxyapatite (HA) discs	224:248	AIM To investigate the null hypothesis that neither the surface conditioning (collagen, serum, saliva) of hydroxyapatite (HA) discs, nor the biofilm age (3 days vs. 21 days) has a significant effect on the cellular and matrix composition of biofilms, using Enterococcus faecalis as the model organism.
31408199	2	5	theme	METHODOLOGY	420:430	arg1	discs					443:447	METHODOLOGY Sterile HA discs	420:447	METHODOLOGY Sterile HA discs	420:447	METHODOLOGY Sterile HA discs were conditioned with collagen, saliva or serum, and inoculated with E. faecalis to form 3-day and 21-day-old biofilms.
31408199	0	6	from	influence	4:12	arg1	composition					71:81	the composition	67:81	the composition of Enterococcus faecalis biofilms	67:115	The influence of substrate surface conditioning and biofilm age on the composition of Enterococcus faecalis biofilms.
31408199	7	7	from	content	1213:1219	arg1	biofilms					1235:1242	the 21-day biofilms	1224:1242	the 21-day biofilms	1224:1242	Although the biochemical analysis revealed that surface conditioning had no significant effect on the total carbohydrate content in the 21-day biofilms, confocal microscopic analysis revealed that collagen and saliva conditioning selectively increased the polysaccharide content of 21-day biofilms, compared to the 3-day biofilms (P < 0.05).
31408199	1	8	dep	saliva	213:218	arg1	serum					206:210	the surface conditioning (collagen, serum, saliva) of hydroxyapatite (HA) discs,	170:249	serum	206:210	AIM To investigate the null hypothesis that neither the surface conditioning (collagen, serum, saliva) of hydroxyapatite (HA) discs, nor the biofilm age (3 days vs. 21 days) has a significant effect on the cellular and matrix composition of biofilms, using Enterococcus faecalis as the model organism.
31408199	8	9	theme	biofilms	1662:1669	arg1	components					1636:1645	the cellular and extracellular matrix components	1598:1645	the cellular and extracellular matrix components of E. faecalis biofilms	1598:1669	CONCLUSIONS The results of this study raise an important methodological concern that the substrate conditioning substances and biofilm age differentially influence the cellular and extracellular matrix components of E. faecalis biofilms.
31408199	7	10	theme	biofilms	1381:1388	arg1	content					1363:1369	the polysaccharide content	1344:1369	the polysaccharide content of 21-day biofilms	1344:1388	Although the biochemical analysis revealed that surface conditioning had no significant effect on the total carbohydrate content in the 21-day biofilms, confocal microscopic analysis revealed that collagen and saliva conditioning selectively increased the polysaccharide content of 21-day biofilms, compared to the 3-day biofilms (P < 0.05).
31408199	5	11	theme	Statistical	851:861	arg1	analyses					863:870	Statistical analyses	851:870	Statistical analyses	851:870	Statistical analyses were performed using appropriate parametric and nonparametric tests (P = 0.05).
31408199	7	12	theme	3-day	1407:1411	arg1	biofilms					1413:1420	the 3-day biofilms	1403:1420	the 3-day biofilms (P < 0.05)	1403:1431	Although the biochemical analysis revealed that surface conditioning had no significant effect on the total carbohydrate content in the 21-day biofilms, confocal microscopic analysis revealed that collagen and saliva conditioning selectively increased the polysaccharide content of 21-day biofilms, compared to the 3-day biofilms (P < 0.05).
31408199	7	12	theme	3-day	1407:1411	arg1	P < 0.05					1423:1430	P < 0.05	1423:1430	P < 0.05	1423:1430	Although the biochemical analysis revealed that surface conditioning had no significant effect on the total carbohydrate content in the 21-day biofilms, confocal microscopic analysis revealed that collagen and saliva conditioning selectively increased the polysaccharide content of 21-day biofilms, compared to the 3-day biofilms (P < 0.05).
31408199	6	13	theme	RESULTS	952:958	arg1	conditioning					969:980	RESULTS Collagen conditioning	952:980	RESULTS Collagen conditioning	952:980	RESULTS Collagen conditioning significantly increased the number of CFUs in the 21-day biofilms, compared to the 3-day biofilms (P < 0.05).
31408199	7	14	theme	confocal	1245:1252	arg1	analysis					1266:1273	confocal microscopic analysis	1245:1273	confocal microscopic analysis	1245:1273	Although the biochemical analysis revealed that surface conditioning had no significant effect on the total carbohydrate content in the 21-day biofilms, confocal microscopic analysis revealed that collagen and saliva conditioning selectively increased the polysaccharide content of 21-day biofilms, compared to the 3-day biofilms (P < 0.05).
31408199	8	15	theme	conditioning	1533:1544	arg1	substances					1546:1555	the substrate conditioning substances	1519:1555	the substrate conditioning substances	1519:1555	CONCLUSIONS The results of this study raise an important methodological concern that the substrate conditioning substances and biofilm age differentially influence the cellular and extracellular matrix components of E. faecalis biofilms.
31408199	6	16	theme	Collagen	960:967	arg1	conditioning					969:980	RESULTS Collagen conditioning	952:980	RESULTS Collagen conditioning	952:980	RESULTS Collagen conditioning significantly increased the number of CFUs in the 21-day biofilms, compared to the 3-day biofilms (P < 0.05).
31408199	7	17	theme	21-day	1228:1233	arg1	biofilms					1235:1242	the 21-day biofilms	1224:1242	the 21-day biofilms	1224:1242	Although the biochemical analysis revealed that surface conditioning had no significant effect on the total carbohydrate content in the 21-day biofilms, confocal microscopic analysis revealed that collagen and saliva conditioning selectively increased the polysaccharide content of 21-day biofilms, compared to the 3-day biofilms (P < 0.05).
31408199	5	18	theme	parametric	905:914	arg1	tests					934:938	appropriate parametric and nonparametric tests	893:938	appropriate parametric and nonparametric tests (P = 0.05)	893:949	Statistical analyses were performed using appropriate parametric and nonparametric tests (P = 0.05).
31408199	5	18	theme	parametric	905:914	arg1	P = 0.05					941:948	P = 0.05	941:948	P = 0.05	941:948	Statistical analyses were performed using appropriate parametric and nonparametric tests (P = 0.05).
31408199	0	19	theme	biofilms	108:115	arg1	composition					71:81	the composition	67:81	the composition of Enterococcus faecalis biofilms	67:115	The influence of substrate surface conditioning and biofilm age on the composition of Enterococcus faecalis biofilms.
31408199	1	20	theme	significant	298:308	arg1	effect					310:315	a significant effect	296:315	a significant effect	296:315	AIM To investigate the null hypothesis that neither the surface conditioning (collagen, serum, saliva) of hydroxyapatite (HA) discs, nor the biofilm age (3 days vs. 21 days) has a significant effect on the cellular and matrix composition of biofilms, using Enterococcus faecalis as the model organism.
31408199	6	21	theme	21-day	1032:1037	arg1	biofilms					1039:1046	the 21-day biofilms	1028:1046	the 21-day biofilms	1028:1046	RESULTS Collagen conditioning significantly increased the number of CFUs in the 21-day biofilms, compared to the 3-day biofilms (P < 0.05).
31408199	0	22	theme	faecalis	99:106	arg1	biofilms					108:115	Enterococcus faecalis biofilms	86:115	Enterococcus faecalis biofilms	86:115	The influence of substrate surface conditioning and biofilm age on the composition of Enterococcus faecalis biofilms.
31408199	7	23	theme	saliva	1302:1307	arg1	conditioning					1309:1320	saliva conditioning	1302:1320	saliva conditioning	1302:1320	Although the biochemical analysis revealed that surface conditioning had no significant effect on the total carbohydrate content in the 21-day biofilms, confocal microscopic analysis revealed that collagen and saliva conditioning selectively increased the polysaccharide content of 21-day biofilms, compared to the 3-day biofilms (P < 0.05).
31408199	8	24	theme	matrix	1629:1634	arg1	components					1636:1645	the cellular and extracellular matrix components	1598:1645	the cellular and extracellular matrix components of E. faecalis biofilms	1598:1669	CONCLUSIONS The results of this study raise an important methodological concern that the substrate conditioning substances and biofilm age differentially influence the cellular and extracellular matrix components of E. faecalis biofilms.
31408199	6	25	theme	3-day	1065:1069	arg1	P < 0.05					1081:1088	P < 0.05	1081:1088	P < 0.05	1081:1088	RESULTS Collagen conditioning significantly increased the number of CFUs in the 21-day biofilms, compared to the 3-day biofilms (P < 0.05).
31408199	6	25	theme	3-day	1065:1069	arg1	biofilms					1071:1078	the 3-day biofilms	1061:1078	the 3-day biofilms (P < 0.05)	1061:1089	RESULTS Collagen conditioning significantly increased the number of CFUs in the 21-day biofilms, compared to the 3-day biofilms (P < 0.05).
31408199	7	26	theme	significant	1168:1178	arg1	effect					1180:1185	no significant effect	1165:1185	no significant effect	1165:1185	Although the biochemical analysis revealed that surface conditioning had no significant effect on the total carbohydrate content in the 21-day biofilms, confocal microscopic analysis revealed that collagen and saliva conditioning selectively increased the polysaccharide content of 21-day biofilms, compared to the 3-day biofilms (P < 0.05).
31408199	1	27	dep	age	267:269	arg1	21 days					283:289	21 days	283:289	21 days	283:289	AIM To investigate the null hypothesis that neither the surface conditioning (collagen, serum, saliva) of hydroxyapatite (HA) discs, nor the biofilm age (3 days vs. 21 days) has a significant effect on the cellular and matrix composition of biofilms, using Enterococcus faecalis as the model organism.
31408199	1	27	dep	age	267:269	arg1	3 days					272:277	3 days	272:277	3 days	272:277	AIM To investigate the null hypothesis that neither the surface conditioning (collagen, serum, saliva) of hydroxyapatite (HA) discs, nor the biofilm age (3 days vs. 21 days) has a significant effect on the cellular and matrix composition of biofilms, using Enterococcus faecalis as the model organism.
31408199	2	28	theme	21-day-old	548:557	arg1	biofilms					559:566	3-day and 21-day-old biofilms	538:566	3-day and 21-day-old biofilms	538:566	METHODOLOGY Sterile HA discs were conditioned with collagen, saliva or serum, and inoculated with E. faecalis to form 3-day and 21-day-old biofilms.
31408199	1	29	theme	model	404:408	arg1	faecalis					388:395	Enterococcus faecalis	375:395	Enterococcus faecalis	375:395	AIM To investigate the null hypothesis that neither the surface conditioning (collagen, serum, saliva) of hydroxyapatite (HA) discs, nor the biofilm age (3 days vs. 21 days) has a significant effect on the cellular and matrix composition of biofilms, using Enterococcus faecalis as the model organism.
31408199	1	29	theme	model	404:408	arg1	organism					410:417	the model organism	400:417	the model organism	400:417	AIM To investigate the null hypothesis that neither the surface conditioning (collagen, serum, saliva) of hydroxyapatite (HA) discs, nor the biofilm age (3 days vs. 21 days) has a significant effect on the cellular and matrix composition of biofilms, using Enterococcus faecalis as the model organism.
31408199	1	30	dep	hypothesis	146:155	arg1	has					292:294	has	292:294	has	292:294	AIM To investigate the null hypothesis that neither the surface conditioning (collagen, serum, saliva) of hydroxyapatite (HA) discs, nor the biofilm age (3 days vs. 21 days) has a significant effect on the cellular and matrix composition of biofilms, using Enterococcus faecalis as the model organism.
31408199	7	31	theme	microscopic	1254:1264	arg1	analysis					1266:1273	confocal microscopic analysis	1245:1273	confocal microscopic analysis	1245:1273	Although the biochemical analysis revealed that surface conditioning had no significant effect on the total carbohydrate content in the 21-day biofilms, confocal microscopic analysis revealed that collagen and saliva conditioning selectively increased the polysaccharide content of 21-day biofilms, compared to the 3-day biofilms (P < 0.05).
31408199	1	32	theme	surface	174:180	arg1	saliva					213:218	the surface conditioning (collagen, serum, saliva) of hydroxyapatite (HA) discs,	170:249	saliva	213:218	AIM To investigate the null hypothesis that neither the surface conditioning (collagen, serum, saliva) of hydroxyapatite (HA) discs, nor the biofilm age (3 days vs. 21 days) has a significant effect on the cellular and matrix composition of biofilms, using Enterococcus faecalis as the model organism.
31408199	8	33	dep	CONCLUSIONS	1434:1444	arg1	raise					1472:1476	raise	1472:1476	raise an important methodological concern that the substrate conditioning substances and biofilm age differentially influence the cellular and extracellular matrix components of E. faecalis biofilms	1472:1669	CONCLUSIONS The results of this study raise an important methodological concern that the substrate conditioning substances and biofilm age differentially influence the cellular and extracellular matrix components of E. faecalis biofilms.
31408199	1	34	theme	discs	244:248	arg1	saliva					213:218	the surface conditioning (collagen, serum, saliva) of hydroxyapatite (HA) discs,	170:249	saliva	213:218	AIM To investigate the null hypothesis that neither the surface conditioning (collagen, serum, saliva) of hydroxyapatite (HA) discs, nor the biofilm age (3 days vs. 21 days) has a significant effect on the cellular and matrix composition of biofilms, using Enterococcus faecalis as the model organism.
31408199	8	35	theme	important	1481:1489	arg1	concern					1506:1512	an important methodological concern that the substrate conditioning substances and biofilm age differentially influence the cellular and extracellular matrix components of E. faecalis biofilms	1478:1669	an important methodological concern that the substrate conditioning substances and biofilm age differentially influence the cellular and extracellular matrix components of E. faecalis biofilms	1478:1669	CONCLUSIONS The results of this study raise an important methodological concern that the substrate conditioning substances and biofilm age differentially influence the cellular and extracellular matrix components of E. faecalis biofilms.
31408199	5	36	theme	nonparametric	920:932	arg1	tests					934:938	appropriate parametric and nonparametric tests	893:938	appropriate parametric and nonparametric tests (P = 0.05)	893:949	Statistical analyses were performed using appropriate parametric and nonparametric tests (P = 0.05).
31408199	5	36	theme	nonparametric	920:932	arg1	P = 0.05					941:948	P = 0.05	941:948	P = 0.05	941:948	Statistical analyses were performed using appropriate parametric and nonparametric tests (P = 0.05).
31408199	1	37	theme	conditioning	182:193	arg1	saliva					213:218	the surface conditioning (collagen, serum, saliva) of hydroxyapatite (HA) discs,	170:249	saliva	213:218	AIM To investigate the null hypothesis that neither the surface conditioning (collagen, serum, saliva) of hydroxyapatite (HA) discs, nor the biofilm age (3 days vs. 21 days) has a significant effect on the cellular and matrix composition of biofilms, using Enterococcus faecalis as the model organism.
31408199	0	38	theme	substrate	17:25	arg1	conditioning					35:46	substrate surface conditioning	17:46	substrate surface conditioning	17:46	The influence of substrate surface conditioning and biofilm age on the composition of Enterococcus faecalis biofilms.
31408199	7	39	theme	polysaccharide	1348:1361	arg1	content					1363:1369	the polysaccharide content	1344:1369	the polysaccharide content of 21-day biofilms	1344:1388	Although the biochemical analysis revealed that surface conditioning had no significant effect on the total carbohydrate content in the 21-day biofilms, confocal microscopic analysis revealed that collagen and saliva conditioning selectively increased the polysaccharide content of 21-day biofilms, compared to the 3-day biofilms (P < 0.05).
31408199	7	40	contain	had	1161:1163	arg1	conditioning					1148:1159	surface conditioning	1140:1159	surface conditioning	1140:1159	Although the biochemical analysis revealed that surface conditioning had no significant effect on the total carbohydrate content in the 21-day biofilms, confocal microscopic analysis revealed that collagen and saliva conditioning selectively increased the polysaccharide content of 21-day biofilms, compared to the 3-day biofilms (P < 0.05).
31408199	7	40	contain	had	1161:1163	arg2	effect					1180:1185	no significant effect	1165:1185	no significant effect	1165:1185	Although the biochemical analysis revealed that surface conditioning had no significant effect on the total carbohydrate content in the 21-day biofilms, confocal microscopic analysis revealed that collagen and saliva conditioning selectively increased the polysaccharide content of 21-day biofilms, compared to the 3-day biofilms (P < 0.05).
31408199	4	41	dep	independent	664:674	arg1	microscopy					686:695	confocal microscopy	677:695	confocal microscopy	677:695	The biofilms were analysed using culture-dependent and independent (confocal microscopy and biochemical analysis) methods, to determine the colony-forming units and the biofilm matrix composition (polysaccharides and proteins), respectively.
31408199	4	41	dep	independent	664:674	arg1	analysis					713:720	biochemical analysis	701:720	biochemical analysis	701:720	The biofilms were analysed using culture-dependent and independent (confocal microscopy and biochemical analysis) methods, to determine the colony-forming units and the biofilm matrix composition (polysaccharides and proteins), respectively.
31408199	4	42	dep	composition	793:803	arg1	polysaccharides					806:820	polysaccharides	806:820	polysaccharides	806:820	The biofilms were analysed using culture-dependent and independent (confocal microscopy and biochemical analysis) methods, to determine the colony-forming units and the biofilm matrix composition (polysaccharides and proteins), respectively.
31408199	4	42	dep	composition	793:803	arg1	proteins					826:833	proteins	826:833	proteins	826:833	The biofilms were analysed using culture-dependent and independent (confocal microscopy and biochemical analysis) methods, to determine the colony-forming units and the biofilm matrix composition (polysaccharides and proteins), respectively.
31408199	8	43	theme	substrate	1523:1531	arg1	substances					1546:1555	the substrate conditioning substances	1519:1555	the substrate conditioning substances	1519:1555	CONCLUSIONS The results of this study raise an important methodological concern that the substrate conditioning substances and biofilm age differentially influence the cellular and extracellular matrix components of E. faecalis biofilms.
31408199	4	44	theme	biochemical	701:711	arg1	analysis					713:720	biochemical analysis	701:720	biochemical analysis	701:720	The biofilms were analysed using culture-dependent and independent (confocal microscopy and biochemical analysis) methods, to determine the colony-forming units and the biofilm matrix composition (polysaccharides and proteins), respectively.
31408199	1	45	theme	collagen	196:203	arg1	saliva					213:218	the surface conditioning (collagen, serum, saliva) of hydroxyapatite (HA) discs,	170:249	saliva	213:218	AIM To investigate the null hypothesis that neither the surface conditioning (collagen, serum, saliva) of hydroxyapatite (HA) discs, nor the biofilm age (3 days vs. 21 days) has a significant effect on the cellular and matrix composition of biofilms, using Enterococcus faecalis as the model organism.
31408199	0	46	theme	conditioning	35:46	arg1	influence					4:12	The influence	0:12	The influence of substrate surface conditioning and biofilm age on the composition of Enterococcus faecalis biofilms	0:115	The influence of substrate surface conditioning and biofilm age on the composition of Enterococcus faecalis biofilms.
31408199	1	47	theme	cellular	324:331	arg1	composition					344:354	the cellular and matrix composition	320:354	composition	344:354	AIM To investigate the null hypothesis that neither the surface conditioning (collagen, serum, saliva) of hydroxyapatite (HA) discs, nor the biofilm age (3 days vs. 21 days) has a significant effect on the cellular and matrix composition of biofilms, using Enterococcus faecalis as the model organism.
31408199	7	48	theme	biochemical	1105:1115	arg1	analysis					1117:1124	the biochemical analysis	1101:1124	the biochemical analysis	1101:1124	Although the biochemical analysis revealed that surface conditioning had no significant effect on the total carbohydrate content in the 21-day biofilms, confocal microscopic analysis revealed that collagen and saliva conditioning selectively increased the polysaccharide content of 21-day biofilms, compared to the 3-day biofilms (P < 0.05).
31408199	0	49	theme	surface	27:33	arg1	conditioning					35:46	substrate surface conditioning	17:46	substrate surface conditioning	17:46	The influence of substrate surface conditioning and biofilm age on the composition of Enterococcus faecalis biofilms.
31408199	1	50	theme	biofilm	259:265	arg1	age					267:269	the biofilm age	255:269	the biofilm age (3 days vs. 21 days)	255:290	AIM To investigate the null hypothesis that neither the surface conditioning (collagen, serum, saliva) of hydroxyapatite (HA) discs, nor the biofilm age (3 days vs. 21 days) has a significant effect on the cellular and matrix composition of biofilms, using Enterococcus faecalis as the model organism.
31408199	3	51	theme	Unconditioned	569:581	arg1	discs					583:587	Unconditioned discs	569:587	Unconditioned discs	569:587	Unconditioned discs served as controls.
31408199	3	51	theme	Unconditioned	569:581	arg1	controls					599:606	controls	599:606	controls	599:606	Unconditioned discs served as controls.
31408199	7	52	theme	surface	1140:1146	arg1	conditioning					1148:1159	surface conditioning	1140:1159	surface conditioning	1140:1159	Although the biochemical analysis revealed that surface conditioning had no significant effect on the total carbohydrate content in the 21-day biofilms, confocal microscopic analysis revealed that collagen and saliva conditioning selectively increased the polysaccharide content of 21-day biofilms, compared to the 3-day biofilms (P < 0.05).
31408199	0	53	theme	biofilm	52:58	arg1	age					60:62	biofilm age	52:62	biofilm age	52:62	The influence of substrate surface conditioning and biofilm age on the composition of Enterococcus faecalis biofilms.
31408199	8	54	theme	cellular	1602:1609	arg1	components					1636:1645	the cellular and extracellular matrix components	1598:1645	the cellular and extracellular matrix components of E. faecalis biofilms	1598:1669	CONCLUSIONS The results of this study raise an important methodological concern that the substrate conditioning substances and biofilm age differentially influence the cellular and extracellular matrix components of E. faecalis biofilms.
31408199	1	55	theme	matrix	337:342	arg1	composition					344:354	the cellular and matrix composition	320:354	composition	344:354	AIM To investigate the null hypothesis that neither the surface conditioning (collagen, serum, saliva) of hydroxyapatite (HA) discs, nor the biofilm age (3 days vs. 21 days) has a significant effect on the cellular and matrix composition of biofilms, using Enterococcus faecalis as the model organism.
31408199	4	56	theme	matrix	786:791	arg1	composition					793:803	the colony-forming units and the biofilm matrix composition	745:803	the colony-forming units and the biofilm matrix composition (polysaccharides and proteins)	745:834	The biofilms were analysed using culture-dependent and independent (confocal microscopy and biochemical analysis) methods, to determine the colony-forming units and the biofilm matrix composition (polysaccharides and proteins), respectively.
31408199	1	57	contain	has	292:294	arg1	age					267:269	the biofilm age	255:269	the biofilm age (3 days vs. 21 days)	255:290	AIM To investigate the null hypothesis that neither the surface conditioning (collagen, serum, saliva) of hydroxyapatite (HA) discs, nor the biofilm age (3 days vs. 21 days) has a significant effect on the cellular and matrix composition of biofilms, using Enterococcus faecalis as the model organism.
31408199	1	57	contain	has	292:294	arg2	effect					310:315	a significant effect	296:315	a significant effect	296:315	AIM To investigate the null hypothesis that neither the surface conditioning (collagen, serum, saliva) of hydroxyapatite (HA) discs, nor the biofilm age (3 days vs. 21 days) has a significant effect on the cellular and matrix composition of biofilms, using Enterococcus faecalis as the model organism.
31408199	1	57	contain	has	292:294	arg1	saliva					213:218	the surface conditioning (collagen, serum, saliva) of hydroxyapatite (HA) discs,	170:249	saliva	213:218	AIM To investigate the null hypothesis that neither the surface conditioning (collagen, serum, saliva) of hydroxyapatite (HA) discs, nor the biofilm age (3 days vs. 21 days) has a significant effect on the cellular and matrix composition of biofilms, using Enterococcus faecalis as the model organism.
31408199	8	58	theme	extracellular	1615:1627	arg1	components					1636:1645	the cellular and extracellular matrix components	1598:1645	the cellular and extracellular matrix components of E. faecalis biofilms	1598:1669	CONCLUSIONS The results of this study raise an important methodological concern that the substrate conditioning substances and biofilm age differentially influence the cellular and extracellular matrix components of E. faecalis biofilms.
31408199	4	59	theme	biofilm	778:784	arg1	matrix					786:791	the biofilm matrix	774:791	the biofilm matrix	774:791	The biofilms were analysed using culture-dependent and independent (confocal microscopy and biochemical analysis) methods, to determine the colony-forming units and the biofilm matrix composition (polysaccharides and proteins), respectively.
31408199	4	60	theme	independent	664:674	arg1	methods					723:729	culture-dependent and independent (confocal microscopy and biochemical analysis) methods	642:729	culture-dependent and independent (confocal microscopy and biochemical analysis) methods	642:729	The biofilms were analysed using culture-dependent and independent (confocal microscopy and biochemical analysis) methods, to determine the colony-forming units and the biofilm matrix composition (polysaccharides and proteins), respectively.
31408199	8	61	theme	study	1466:1470	arg1	results					1450:1456	The results	1446:1456	The results of this study	1446:1470	CONCLUSIONS The results of this study raise an important methodological concern that the substrate conditioning substances and biofilm age differentially influence the cellular and extracellular matrix components of E. faecalis biofilms.
31408199	4	62	theme	confocal	677:684	arg1	microscopy					686:695	confocal microscopy	677:695	confocal microscopy	677:695	The biofilms were analysed using culture-dependent and independent (confocal microscopy and biochemical analysis) methods, to determine the colony-forming units and the biofilm matrix composition (polysaccharides and proteins), respectively.
31408199	6	63	theme	CFUs	1020:1023	arg1	number					1010:1015	the number	1006:1015	the number of CFUs	1006:1023	RESULTS Collagen conditioning significantly increased the number of CFUs in the 21-day biofilms, compared to the 3-day biofilms (P < 0.05).
31408199	7	64	theme	total	1194:1198	arg1	content					1213:1219	the total carbohydrate content	1190:1219	the total carbohydrate content in the 21-day biofilms	1190:1242	Although the biochemical analysis revealed that surface conditioning had no significant effect on the total carbohydrate content in the 21-day biofilms, confocal microscopic analysis revealed that collagen and saliva conditioning selectively increased the polysaccharide content of 21-day biofilms, compared to the 3-day biofilms (P < 0.05).
31408199	1	65	theme	biofilms	359:366	arg1	composition					344:354	the cellular and matrix composition	320:354	composition	344:354	AIM To investigate the null hypothesis that neither the surface conditioning (collagen, serum, saliva) of hydroxyapatite (HA) discs, nor the biofilm age (3 days vs. 21 days) has a significant effect on the cellular and matrix composition of biofilms, using Enterococcus faecalis as the model organism.
31408199	7	66	theme	21-day	1374:1379	arg1	biofilms					1381:1388	21-day biofilms	1374:1388	21-day biofilms	1374:1388	Although the biochemical analysis revealed that surface conditioning had no significant effect on the total carbohydrate content in the 21-day biofilms, confocal microscopic analysis revealed that collagen and saliva conditioning selectively increased the polysaccharide content of 21-day biofilms, compared to the 3-day biofilms (P < 0.05).
31408199	8	67	theme	methodological	1491:1504	arg1	concern					1506:1512	an important methodological concern that the substrate conditioning substances and biofilm age differentially influence the cellular and extracellular matrix components of E. faecalis biofilms	1478:1669	an important methodological concern that the substrate conditioning substances and biofilm age differentially influence the cellular and extracellular matrix components of E. faecalis biofilms	1478:1669	CONCLUSIONS The results of this study raise an important methodological concern that the substrate conditioning substances and biofilm age differentially influence the cellular and extracellular matrix components of E. faecalis biofilms.
31408199	2	68	theme	HA	440:441	arg1	discs					443:447	METHODOLOGY Sterile HA discs	420:447	METHODOLOGY Sterile HA discs	420:447	METHODOLOGY Sterile HA discs were conditioned with collagen, saliva or serum, and inoculated with E. faecalis to form 3-day and 21-day-old biofilms.
31408199	5	69	theme	appropriate	893:903	arg1	tests					934:938	appropriate parametric and nonparametric tests	893:938	appropriate parametric and nonparametric tests (P = 0.05)	893:949	Statistical analyses were performed using appropriate parametric and nonparametric tests (P = 0.05).
31408199	5	69	theme	appropriate	893:903	arg1	P = 0.05					941:948	P = 0.05	941:948	P = 0.05	941:948	Statistical analyses were performed using appropriate parametric and nonparametric tests (P = 0.05).
31408199	4	70	theme	units	764:768	arg1	composition					793:803	the colony-forming units and the biofilm matrix composition	745:803	the colony-forming units and the biofilm matrix composition (polysaccharides and proteins)	745:834	The biofilms were analysed using culture-dependent and independent (confocal microscopy and biochemical analysis) methods, to determine the colony-forming units and the biofilm matrix composition (polysaccharides and proteins), respectively.
31408199	8	71	theme	biofilm	1561:1567	arg1	age					1569:1571	biofilm age	1561:1571	biofilm age	1561:1571	CONCLUSIONS The results of this study raise an important methodological concern that the substrate conditioning substances and biofilm age differentially influence the cellular and extracellular matrix components of E. faecalis biofilms.
31408199	8	72	theme	E. faecalis	1650:1660	arg1	biofilms					1662:1669	E. faecalis biofilms	1650:1669	E. faecalis biofilms	1650:1669	CONCLUSIONS The results of this study raise an important methodological concern that the substrate conditioning substances and biofilm age differentially influence the cellular and extracellular matrix components of E. faecalis biofilms.
31408199	0	73	theme	age	60:62	arg1	influence					4:12	The influence	0:12	The influence of substrate surface conditioning and biofilm age on the composition of Enterococcus faecalis biofilms	0:115	The influence of substrate surface conditioning and biofilm age on the composition of Enterococcus faecalis biofilms.
31408199	4	74	theme	colony-forming	749:762	arg1	units					764:768	colony-forming units	749:768	colony-forming units	749:768	The biofilms were analysed using culture-dependent and independent (confocal microscopy and biochemical analysis) methods, to determine the colony-forming units and the biofilm matrix composition (polysaccharides and proteins), respectively.
31408199	1	75	theme	null	141:144	arg1	hypothesis					146:155	the null hypothesis	137:155	the null hypothesis that neither the surface conditioning (collagen, serum, saliva) of hydroxyapatite (HA) discs, nor the biofilm age (3 days vs. 21 days) has a significant effect on the cellular and matrix composition of biofilms, using Enterococcus faecalis as the model organism	137:417	AIM To investigate the null hypothesis that neither the surface conditioning (collagen, serum, saliva) of hydroxyapatite (HA) discs, nor the biofilm age (3 days vs. 21 days) has a significant effect on the cellular and matrix composition of biofilms, using Enterococcus faecalis as the model organism.
29969287	10	0	theme	genus	1318:1322	arg1	Lactobacillus					1324:1336	the genus Lactobacillus	1314:1336	the genus Lactobacillus	1314:1336	Ileal microbiota composition of birds fed oat hulls differed from the other groups, as shown by a higher abundance of the genus Enterococcus, mainly at the expense of the genus Lactobacillus.
29969287	0	1	theme	broilers	151:158	arg1	chickens					160:167	broilers chickens	151:167	broilers chickens	151:167	Effect of nutritional interventions with quercetin, oat hulls, β-glucans, lysozyme and fish oil on performance and health status related parameters of broilers chickens.
29969287	3	2	theme	feed	448:451	arg1	ingredients					453:463	the feed ingredients	444:463	the feed ingredients	444:463	It was hypothesized that the feed ingredients would improve health status related parameters.
29969287	9	3	from	effect	1056:1061	arg1	diversity					1098:1106	the microbiota diversity	1083:1106	the microbiota diversity in the jejunum and ileum	1083:1131	No effect of interventions on the microbiota diversity in the jejunum and ileum was observed.
29969287	2	4	with	diets	341:345	arg1	rate					369:372	a high inclusion rate	352:372	a high inclusion rate of rapeseed meal	352:389	An experiment was conducted to evaluate the effects of technical feed ingredients between 14 and 28 d of age on performance and health status of broilers (d 14-35) fed diets with a high inclusion rate of rapeseed meal as a nutritional challenge.
29969287	18	5	theme	related	2172:2178	arg1	parameters					2180:2189	health status related parameters	2158:2189	health status related parameters	2158:2189	It was concluded that the tested nutritional interventions in the current experiment only marginally effected health status related parameters.
29969287	5	6	theme	iso-caloric	629:639	arg1	d 15-28					672:678	d 15-28	672:678	d 15-28	672:678	A total of 1008 one-day-old male Ross 308 chicks were distributed over 36 floor pens and allocated to one of six iso-caloric (AMEN 13 MJ/kg) growing diets (d 15-28): a control and five test diets supplemented with quercetin (400 mg/kg), oat hulls (50 g/kg), β-glucan (100 mg/kg), lysozyme (40 mg/kg) or fish oil ω-3 fatty acids (40 g/kg), with six replicate pens per treatment.
29969287	5	6	theme	iso-caloric	629:639	arg1	diets					665:669	one of six iso-caloric (AMEN 13 MJ/kg) growing diets	618:669	one of six iso-caloric (AMEN 13 MJ/kg) growing diets (d 15-28): a control and five test diets supplemented with quercetin (400 mg/kg), oat hulls (50 g/kg), β-glucan (100 mg/kg), lysozyme (40 mg/kg) or fish oil ω-3 fatty acids (40 g/kg), with six replicate pens per treatment	618:891	A total of 1008 one-day-old male Ross 308 chicks were distributed over 36 floor pens and allocated to one of six iso-caloric (AMEN 13 MJ/kg) growing diets (d 15-28): a control and five test diets supplemented with quercetin (400 mg/kg), oat hulls (50 g/kg), β-glucan (100 mg/kg), lysozyme (40 mg/kg) or fish oil ω-3 fatty acids (40 g/kg), with six replicate pens per treatment.
29969287	7	7	theme	lysozyme	932:939	arg1	inclusion					905:913	Dietary inclusion	897:913	Dietary inclusion of oat hulls and lysozyme	897:939	Dietary inclusion of oat hulls and lysozyme resulted in a reduction in broiler performance during the first week after providing the experimental diets.
29969287	3	8	theme	related	493:499	arg1	parameters					501:510	health status related parameters	479:510	health status related parameters	479:510	It was hypothesized that the feed ingredients would improve health status related parameters.
29969287	18	9	from	interventions	2093:2105	arg1	experiment					2122:2131	the current experiment	2110:2131	the current experiment	2110:2131	It was concluded that the tested nutritional interventions in the current experiment only marginally effected health status related parameters.
29969287	18	10	theme	health	2158:2163	arg1	status					2165:2170	health status	2158:2170	health status	2158:2170	It was concluded that the tested nutritional interventions in the current experiment only marginally effected health status related parameters.
29969287	15	11	from	pathway	1847:1853	arg1	birds					1890:1894	the quercetin- and oat hull-fed birds	1858:1894	the quercetin- and oat hull-fed birds	1858:1894	Genes related to the growth-factor-activity pathway were more highly expressed in birds fed β-glucan compared to the control group, while the genes related to anion-transmembrane-transporter-activity pathway in the quercetin- and oat hull-fed birds were less expressed.
29969287	7	12	theme	hulls	922:926	arg1	inclusion					905:913	Dietary inclusion	897:913	Dietary inclusion of oat hulls and lysozyme	897:939	Dietary inclusion of oat hulls and lysozyme resulted in a reduction in broiler performance during the first week after providing the experimental diets.
29969287	2	13	theme	ingredients	243:253	arg1	challenge					408:416	a nutritional challenge	394:416	a nutritional challenge	394:416	An experiment was conducted to evaluate the effects of technical feed ingredients between 14 and 28 d of age on performance and health status of broilers (d 14-35) fed diets with a high inclusion rate of rapeseed meal as a nutritional challenge.
29969287	2	13	theme	ingredients	243:253	arg1	effects					217:223	the effects	213:223	the effects of technical feed ingredients between 14 and 28 d of age on performance and health status of broilers (d 14-35) fed diets with a high inclusion rate of rapeseed meal	213:389	An experiment was conducted to evaluate the effects of technical feed ingredients between 14 and 28 d of age on performance and health status of broilers (d 14-35) fed diets with a high inclusion rate of rapeseed meal as a nutritional challenge.
29969287	5	14	theme	oat	753:755	arg1	50 g/kg					764:770	50 g/kg	764:770	50 g/kg	764:770	A total of 1008 one-day-old male Ross 308 chicks were distributed over 36 floor pens and allocated to one of six iso-caloric (AMEN 13 MJ/kg) growing diets (d 15-28): a control and five test diets supplemented with quercetin (400 mg/kg), oat hulls (50 g/kg), β-glucan (100 mg/kg), lysozyme (40 mg/kg) or fish oil ω-3 fatty acids (40 g/kg), with six replicate pens per treatment.
29969287	5	14	theme	oat	753:755	arg1	40 g/kg					845:851	40 g/kg	845:851	40 g/kg	845:851	A total of 1008 one-day-old male Ross 308 chicks were distributed over 36 floor pens and allocated to one of six iso-caloric (AMEN 13 MJ/kg) growing diets (d 15-28): a control and five test diets supplemented with quercetin (400 mg/kg), oat hulls (50 g/kg), β-glucan (100 mg/kg), lysozyme (40 mg/kg) or fish oil ω-3 fatty acids (40 g/kg), with six replicate pens per treatment.
29969287	5	14	theme	oat	753:755	arg1	hulls					757:761	oat hulls	753:761	oat hulls (50 g/kg)	753:771	A total of 1008 one-day-old male Ross 308 chicks were distributed over 36 floor pens and allocated to one of six iso-caloric (AMEN 13 MJ/kg) growing diets (d 15-28): a control and five test diets supplemented with quercetin (400 mg/kg), oat hulls (50 g/kg), β-glucan (100 mg/kg), lysozyme (40 mg/kg) or fish oil ω-3 fatty acids (40 g/kg), with six replicate pens per treatment.
29969287	5	14	theme	oat	753:755	arg1	quercetin					730:738	quercetin	730:738	quercetin (400 mg/kg)	730:750	A total of 1008 one-day-old male Ross 308 chicks were distributed over 36 floor pens and allocated to one of six iso-caloric (AMEN 13 MJ/kg) growing diets (d 15-28): a control and five test diets supplemented with quercetin (400 mg/kg), oat hulls (50 g/kg), β-glucan (100 mg/kg), lysozyme (40 mg/kg) or fish oil ω-3 fatty acids (40 g/kg), with six replicate pens per treatment.
29969287	13	15	located	observed	1583:1590	arg2	area					1485:1488	Higher total surface area	1464:1488	Higher total surface area of villi occupied by goblet cells	1464:1522	Higher total surface area of villi occupied by goblet cells and total villi surface area in jejunum (d 21 and 28) were observed in chickens fed oat hulls compared to other groups.
29969287	13	15	located	observed	1583:1590	arg2	area					1548:1551	total villi surface area	1528:1551	total villi surface area in jejunum (d 21 and 28)	1528:1576	Higher total surface area of villi occupied by goblet cells and total villi surface area in jejunum (d 21 and 28) were observed in chickens fed oat hulls compared to other groups.
29969287	13	15	located	observed	1583:1590	arg1	chickens					1595:1602	chickens	1595:1602	chickens fed oat hulls compared to other groups	1595:1641	Higher total surface area of villi occupied by goblet cells and total villi surface area in jejunum (d 21 and 28) were observed in chickens fed oat hulls compared to other groups.
29969287	2	16	theme	age	278:280	arg1	14					263:264	14	263:264	14	263:264	An experiment was conducted to evaluate the effects of technical feed ingredients between 14 and 28 d of age on performance and health status of broilers (d 14-35) fed diets with a high inclusion rate of rapeseed meal as a nutritional challenge.
29969287	2	16	theme	age	278:280	arg1	age					278:280	age	278:280	age	278:280	An experiment was conducted to evaluate the effects of technical feed ingredients between 14 and 28 d of age on performance and health status of broilers (d 14-35) fed diets with a high inclusion rate of rapeseed meal as a nutritional challenge.
29969287	5	17	theme	floor	590:594	arg1	pens					596:599	36 floor pens	587:599	36 floor pens	587:599	A total of 1008 one-day-old male Ross 308 chicks were distributed over 36 floor pens and allocated to one of six iso-caloric (AMEN 13 MJ/kg) growing diets (d 15-28): a control and five test diets supplemented with quercetin (400 mg/kg), oat hulls (50 g/kg), β-glucan (100 mg/kg), lysozyme (40 mg/kg) or fish oil ω-3 fatty acids (40 g/kg), with six replicate pens per treatment.
29969287	12	18	theme	crypt	1376:1380	arg1	depth					1382:1386	crypt depth	1376:1386	crypt depth	1376:1386	In the jejunum, villus height and crypt depth of lysozyme-fed birds at d 28 were decreased compared to the control group.
29969287	13	19	theme	villi	1534:1538	arg1	area					1548:1551	total villi surface area	1528:1551	total villi surface area in jejunum (d 21 and 28)	1528:1576	Higher total surface area of villi occupied by goblet cells and total villi surface area in jejunum (d 21 and 28) were observed in chickens fed oat hulls compared to other groups.
29969287	7	20	theme	Dietary	897:903	arg1	inclusion					905:913	Dietary inclusion	897:913	Dietary inclusion of oat hulls and lysozyme	897:939	Dietary inclusion of oat hulls and lysozyme resulted in a reduction in broiler performance during the first week after providing the experimental diets.
29969287	5	21	theme	AMEN	642:645	arg1	13 MJ/kg					647:654	AMEN 13 MJ/kg	642:654	AMEN 13 MJ/kg	642:654	A total of 1008 one-day-old male Ross 308 chicks were distributed over 36 floor pens and allocated to one of six iso-caloric (AMEN 13 MJ/kg) growing diets (d 15-28): a control and five test diets supplemented with quercetin (400 mg/kg), oat hulls (50 g/kg), β-glucan (100 mg/kg), lysozyme (40 mg/kg) or fish oil ω-3 fatty acids (40 g/kg), with six replicate pens per treatment.
29969287	9	22	from	diversity	1098:1106	arg1	ileum					1127:1131	ileum	1127:1131	ileum	1127:1131	No effect of interventions on the microbiota diversity in the jejunum and ileum was observed.
29969287	9	22	from	diversity	1098:1106	arg1	jejunum					1115:1121	jejunum	1115:1121	jejunum	1115:1121	No effect of interventions on the microbiota diversity in the jejunum and ileum was observed.
29969287	13	23	theme	goblet	1511:1516	arg1	cells					1518:1522	goblet cells	1511:1522	goblet cells	1511:1522	Higher total surface area of villi occupied by goblet cells and total villi surface area in jejunum (d 21 and 28) were observed in chickens fed oat hulls compared to other groups.
29969287	0	24	theme	status	122:127	arg1	parameters					137:146	performance and health status related parameters	99:146	performance and health status related parameters of broilers chickens	99:167	Effect of nutritional interventions with quercetin, oat hulls, β-glucans, lysozyme and fish oil on performance and health status related parameters of broilers chickens.
29969287	9	25	theme	interventions	1066:1078	arg1	effect					1056:1061	No effect	1053:1061	No effect of interventions on the microbiota diversity in the jejunum and ileum	1053:1131	No effect of interventions on the microbiota diversity in the jejunum and ileum was observed.
29969287	16	26	theme	dietary	1957:1963	arg1	interventions					1965:1977	dietary interventions	1957:1977	dietary interventions	1957:1977	The genes differently expressed between dietary interventions did not seem to be directly involved in immune related processes.
29969287	2	27	theme	technical	228:236	arg1	ingredients					243:253	technical feed ingredients	228:253	technical feed ingredients between 14 and 28 d of age	228:280	An experiment was conducted to evaluate the effects of technical feed ingredients between 14 and 28 d of age on performance and health status of broilers (d 14-35) fed diets with a high inclusion rate of rapeseed meal as a nutritional challenge.
29969287	12	28	theme	birds	1404:1408	arg1	height					1365:1370	villus height	1358:1370	villus height	1358:1370	In the jejunum, villus height and crypt depth of lysozyme-fed birds at d 28 were decreased compared to the control group.
29969287	12	28	theme	birds	1404:1408	arg1	depth					1382:1386	crypt depth	1376:1386	crypt depth	1376:1386	In the jejunum, villus height and crypt depth of lysozyme-fed birds at d 28 were decreased compared to the control group.
29969287	7	29	from	reduction	955:963	arg1	performance					976:986	broiler performance	968:986	broiler performance during the first week after providing the experimental diets	968:1047	Dietary inclusion of oat hulls and lysozyme resulted in a reduction in broiler performance during the first week after providing the experimental diets.
29969287	10	30	theme	higher	1245:1250	arg1	abundance					1252:1260	a higher abundance	1243:1260	a higher abundance of the genus Enterococcus	1243:1286	Ileal microbiota composition of birds fed oat hulls differed from the other groups, as shown by a higher abundance of the genus Enterococcus, mainly at the expense of the genus Lactobacillus.
29969287	5	31	theme	one-day-old	532:542	arg1	chicks					558:563	1008 one-day-old male Ross 308 chicks	527:563	1008 one-day-old male Ross 308 chicks	527:563	A total of 1008 one-day-old male Ross 308 chicks were distributed over 36 floor pens and allocated to one of six iso-caloric (AMEN 13 MJ/kg) growing diets (d 15-28): a control and five test diets supplemented with quercetin (400 mg/kg), oat hulls (50 g/kg), β-glucan (100 mg/kg), lysozyme (40 mg/kg) or fish oil ω-3 fatty acids (40 g/kg), with six replicate pens per treatment.
29969287	0	32	from	Effect	0:5	arg1	parameters					137:146	performance and health status related parameters	99:146	performance and health status related parameters of broilers chickens	99:167	Effect of nutritional interventions with quercetin, oat hulls, β-glucans, lysozyme and fish oil on performance and health status related parameters of broilers chickens.
29969287	5	33	theme	Ross	549:552	arg1	chicks					558:563	1008 one-day-old male Ross 308 chicks	527:563	1008 one-day-old male Ross 308 chicks	527:563	A total of 1008 one-day-old male Ross 308 chicks were distributed over 36 floor pens and allocated to one of six iso-caloric (AMEN 13 MJ/kg) growing diets (d 15-28): a control and five test diets supplemented with quercetin (400 mg/kg), oat hulls (50 g/kg), β-glucan (100 mg/kg), lysozyme (40 mg/kg) or fish oil ω-3 fatty acids (40 g/kg), with six replicate pens per treatment.
29969287	5	34	theme	fish	819:822	arg1	quercetin					730:738	quercetin	730:738	quercetin (400 mg/kg)	730:750	A total of 1008 one-day-old male Ross 308 chicks were distributed over 36 floor pens and allocated to one of six iso-caloric (AMEN 13 MJ/kg) growing diets (d 15-28): a control and five test diets supplemented with quercetin (400 mg/kg), oat hulls (50 g/kg), β-glucan (100 mg/kg), lysozyme (40 mg/kg) or fish oil ω-3 fatty acids (40 g/kg), with six replicate pens per treatment.
29969287	5	34	theme	fish	819:822	arg1	acids					838:842	fish oil ω-3 fatty acids	819:842	fish oil ω-3 fatty acids	819:842	A total of 1008 one-day-old male Ross 308 chicks were distributed over 36 floor pens and allocated to one of six iso-caloric (AMEN 13 MJ/kg) growing diets (d 15-28): a control and five test diets supplemented with quercetin (400 mg/kg), oat hulls (50 g/kg), β-glucan (100 mg/kg), lysozyme (40 mg/kg) or fish oil ω-3 fatty acids (40 g/kg), with six replicate pens per treatment.
29969287	10	35	theme	genus	1269:1273	arg1	Enterococcus					1275:1286	the genus Enterococcus	1265:1286	the genus Enterococcus	1265:1286	Ileal microbiota composition of birds fed oat hulls differed from the other groups, as shown by a higher abundance of the genus Enterococcus, mainly at the expense of the genus Lactobacillus.
29969287	0	36	theme	fish	87:90	arg1	oil					92:94	fish oil	87:94	fish oil	87:94	Effect of nutritional interventions with quercetin, oat hulls, β-glucans, lysozyme and fish oil on performance and health status related parameters of broilers chickens.
29969287	5	37	theme	ω-3	828:830	arg1	quercetin					730:738	quercetin	730:738	quercetin (400 mg/kg)	730:750	A total of 1008 one-day-old male Ross 308 chicks were distributed over 36 floor pens and allocated to one of six iso-caloric (AMEN 13 MJ/kg) growing diets (d 15-28): a control and five test diets supplemented with quercetin (400 mg/kg), oat hulls (50 g/kg), β-glucan (100 mg/kg), lysozyme (40 mg/kg) or fish oil ω-3 fatty acids (40 g/kg), with six replicate pens per treatment.
29969287	5	37	theme	ω-3	828:830	arg1	acids					838:842	fish oil ω-3 fatty acids	819:842	fish oil ω-3 fatty acids	819:842	A total of 1008 one-day-old male Ross 308 chicks were distributed over 36 floor pens and allocated to one of six iso-caloric (AMEN 13 MJ/kg) growing diets (d 15-28): a control and five test diets supplemented with quercetin (400 mg/kg), oat hulls (50 g/kg), β-glucan (100 mg/kg), lysozyme (40 mg/kg) or fish oil ω-3 fatty acids (40 g/kg), with six replicate pens per treatment.
29969287	15	38	theme	related	1653:1659	arg1	Genes					1647:1651	Genes	1647:1651	Genes related to the growth-factor-activity pathway	1647:1697	Genes related to the growth-factor-activity pathway were more highly expressed in birds fed β-glucan compared to the control group, while the genes related to anion-transmembrane-transporter-activity pathway in the quercetin- and oat hull-fed birds were less expressed.
29969287	7	39	theme	first	999:1003	arg1	week					1005:1008	the first week	995:1008	the first week after providing the experimental diets	995:1047	Dietary inclusion of oat hulls and lysozyme resulted in a reduction in broiler performance during the first week after providing the experimental diets.
29969287	7	40	theme	oat	918:920	arg1	hulls					922:926	oat hulls	918:926	oat hulls	918:926	Dietary inclusion of oat hulls and lysozyme resulted in a reduction in broiler performance during the first week after providing the experimental diets.
29969287	2	41	theme	rapeseed	377:384	arg1	meal					386:389	rapeseed meal	377:389	rapeseed meal	377:389	An experiment was conducted to evaluate the effects of technical feed ingredients between 14 and 28 d of age on performance and health status of broilers (d 14-35) fed diets with a high inclusion rate of rapeseed meal as a nutritional challenge.
29969287	9	42	dep	jejunum	1115:1121	arg1	the					1111:1113	the	1111:1113	the	1111:1113	No effect of interventions on the microbiota diversity in the jejunum and ileum was observed.
29969287	16	43	theme	immune	2019:2024	arg1	processes					2034:2042	immune related processes	2019:2042	immune related processes	2019:2042	The genes differently expressed between dietary interventions did not seem to be directly involved in immune related processes.
29969287	0	44	theme	nutritional	10:20	arg1	interventions					22:34	nutritional interventions	10:34	nutritional interventions with quercetin, oat hulls, β-glucans, lysozyme and fish oil	10:94	Effect of nutritional interventions with quercetin, oat hulls, β-glucans, lysozyme and fish oil on performance and health status related parameters of broilers chickens.
29969287	2	45	theme	high	354:357	arg1	rate					369:372	a high inclusion rate	352:372	a high inclusion rate of rapeseed meal	352:389	An experiment was conducted to evaluate the effects of technical feed ingredients between 14 and 28 d of age on performance and health status of broilers (d 14-35) fed diets with a high inclusion rate of rapeseed meal as a nutritional challenge.
29969287	7	46	theme	broiler	968:974	arg1	performance					976:986	broiler performance	968:986	broiler performance during the first week after providing the experimental diets	968:1047	Dietary inclusion of oat hulls and lysozyme resulted in a reduction in broiler performance during the first week after providing the experimental diets.
29969287	13	47	from	area	1485:1488	arg1	jejunum					1556:1562	jejunum	1556:1562	jejunum	1556:1562	Higher total surface area of villi occupied by goblet cells and total villi surface area in jejunum (d 21 and 28) were observed in chickens fed oat hulls compared to other groups.
29969287	15	48	theme	hull-fed	1881:1888	arg1	birds					1890:1894	the quercetin- and oat hull-fed birds	1858:1894	the quercetin- and oat hull-fed birds	1858:1894	Genes related to the growth-factor-activity pathway were more highly expressed in birds fed β-glucan compared to the control group, while the genes related to anion-transmembrane-transporter-activity pathway in the quercetin- and oat hull-fed birds were less expressed.
29969287	2	49	theme	nutritional	396:406	arg1	challenge					408:416	a nutritional challenge	394:416	a nutritional challenge	394:416	An experiment was conducted to evaluate the effects of technical feed ingredients between 14 and 28 d of age on performance and health status of broilers (d 14-35) fed diets with a high inclusion rate of rapeseed meal as a nutritional challenge.
29969287	2	49	theme	nutritional	396:406	arg1	effects					217:223	the effects	213:223	the effects of technical feed ingredients between 14 and 28 d of age on performance and health status of broilers (d 14-35) fed diets with a high inclusion rate of rapeseed meal	213:389	An experiment was conducted to evaluate the effects of technical feed ingredients between 14 and 28 d of age on performance and health status of broilers (d 14-35) fed diets with a high inclusion rate of rapeseed meal as a nutritional challenge.
29969287	10	50	theme	microbiota	1153:1162	arg1	composition					1164:1174	Ileal microbiota composition	1147:1174	Ileal microbiota composition of birds fed oat hulls	1147:1197	Ileal microbiota composition of birds fed oat hulls differed from the other groups, as shown by a higher abundance of the genus Enterococcus, mainly at the expense of the genus Lactobacillus.
29969287	3	51	theme	health	479:484	arg1	parameters					501:510	health status related parameters	479:510	health status related parameters	479:510	It was hypothesized that the feed ingredients would improve health status related parameters.
29969287	10	52	theme	Lactobacillus	1324:1336	arg1	expense					1303:1309	the expense	1299:1309	the expense of the genus Lactobacillus	1299:1336	Ileal microbiota composition of birds fed oat hulls differed from the other groups, as shown by a higher abundance of the genus Enterococcus, mainly at the expense of the genus Lactobacillus.
29969287	0	53	theme	chickens	160:167	arg1	parameters					137:146	performance and health status related parameters	99:146	performance and health status related parameters of broilers chickens	99:167	Effect of nutritional interventions with quercetin, oat hulls, β-glucans, lysozyme and fish oil on performance and health status related parameters of broilers chickens.
29969287	2	54	theme	broilers	318:325	arg1	status					308:313	performance and health status	285:313	status	308:313	An experiment was conducted to evaluate the effects of technical feed ingredients between 14 and 28 d of age on performance and health status of broilers (d 14-35) fed diets with a high inclusion rate of rapeseed meal as a nutritional challenge.
29969287	13	55	theme	total	1471:1475	arg1	area					1485:1488	Higher total surface area	1464:1488	Higher total surface area of villi occupied by goblet cells	1464:1522	Higher total surface area of villi occupied by goblet cells and total villi surface area in jejunum (d 21 and 28) were observed in chickens fed oat hulls compared to other groups.
29969287	13	56	theme	surface	1540:1546	arg1	area					1548:1551	total villi surface area	1528:1551	total villi surface area in jejunum (d 21 and 28)	1528:1576	Higher total surface area of villi occupied by goblet cells and total villi surface area in jejunum (d 21 and 28) were observed in chickens fed oat hulls compared to other groups.
29969287	3	57	theme	status	486:491	arg1	parameters					501:510	health status related parameters	479:510	health status related parameters	479:510	It was hypothesized that the feed ingredients would improve health status related parameters.
29969287	5	58	dep	pens	874:877	arg1	replicate					864:872	replicate	864:872	replicate	864:872	A total of 1008 one-day-old male Ross 308 chicks were distributed over 36 floor pens and allocated to one of six iso-caloric (AMEN 13 MJ/kg) growing diets (d 15-28): a control and five test diets supplemented with quercetin (400 mg/kg), oat hulls (50 g/kg), β-glucan (100 mg/kg), lysozyme (40 mg/kg) or fish oil ω-3 fatty acids (40 g/kg), with six replicate pens per treatment.
29969287	5	59	theme	chicks	558:563	arg1	total					518:522	A total	516:522	A total of 1008 one-day-old male Ross 308 chicks	516:563	A total of 1008 one-day-old male Ross 308 chicks were distributed over 36 floor pens and allocated to one of six iso-caloric (AMEN 13 MJ/kg) growing diets (d 15-28): a control and five test diets supplemented with quercetin (400 mg/kg), oat hulls (50 g/kg), β-glucan (100 mg/kg), lysozyme (40 mg/kg) or fish oil ω-3 fatty acids (40 g/kg), with six replicate pens per treatment.
29969287	12	60	from	d 28	1413:1416	arg1	height					1365:1370	villus height	1358:1370	villus height	1358:1370	In the jejunum, villus height and crypt depth of lysozyme-fed birds at d 28 were decreased compared to the control group.
29969287	12	60	from	d 28	1413:1416	arg1	depth					1382:1386	crypt depth	1376:1386	crypt depth	1376:1386	In the jejunum, villus height and crypt depth of lysozyme-fed birds at d 28 were decreased compared to the control group.
29969287	13	61	theme	total	1528:1532	arg1	area					1548:1551	total villi surface area	1528:1551	total villi surface area in jejunum (d 21 and 28)	1528:1576	Higher total surface area of villi occupied by goblet cells and total villi surface area in jejunum (d 21 and 28) were observed in chickens fed oat hulls compared to other groups.
29969287	0	62	theme	performance	99:109	arg1	parameters					137:146	performance and health status related parameters	99:146	performance and health status related parameters of broilers chickens	99:167	Effect of nutritional interventions with quercetin, oat hulls, β-glucans, lysozyme and fish oil on performance and health status related parameters of broilers chickens.
29969287	10	63	theme	other	1217:1221	arg1	groups					1223:1228	the other groups	1213:1228	the other groups	1213:1228	Ileal microbiota composition of birds fed oat hulls differed from the other groups, as shown by a higher abundance of the genus Enterococcus, mainly at the expense of the genus Lactobacillus.
29969287	12	64	theme	lysozyme-fed	1391:1402	arg1	birds					1404:1408	lysozyme-fed birds	1391:1408	lysozyme-fed birds	1391:1408	In the jejunum, villus height and crypt depth of lysozyme-fed birds at d 28 were decreased compared to the control group.
29969287	13	65	theme	villi	1493:1497	arg1	area					1485:1488	Higher total surface area	1464:1488	Higher total surface area of villi occupied by goblet cells	1464:1522	Higher total surface area of villi occupied by goblet cells and total villi surface area in jejunum (d 21 and 28) were observed in chickens fed oat hulls compared to other groups.
29969287	13	65	theme	villi	1493:1497	arg1	area					1548:1551	total villi surface area	1528:1551	total villi surface area in jejunum (d 21 and 28)	1528:1576	Higher total surface area of villi occupied by goblet cells and total villi surface area in jejunum (d 21 and 28) were observed in chickens fed oat hulls compared to other groups.
29969287	0	66	theme	health	115:120	arg1	status					122:127	health status	115:127	health status	115:127	Effect of nutritional interventions with quercetin, oat hulls, β-glucans, lysozyme and fish oil on performance and health status related parameters of broilers chickens.
29969287	12	67	theme	villus	1358:1363	arg1	height					1365:1370	villus height	1358:1370	villus height	1358:1370	In the jejunum, villus height and crypt depth of lysozyme-fed birds at d 28 were decreased compared to the control group.
29969287	0	68	theme	related	129:135	arg1	parameters					137:146	performance and health status related parameters	99:146	performance and health status related parameters of broilers chickens	99:167	Effect of nutritional interventions with quercetin, oat hulls, β-glucans, lysozyme and fish oil on performance and health status related parameters of broilers chickens.
29969287	13	69	from	area	1548:1551	arg1	jejunum					1556:1562	jejunum	1556:1562	jejunum	1556:1562	Higher total surface area of villi occupied by goblet cells and total villi surface area in jejunum (d 21 and 28) were observed in chickens fed oat hulls compared to other groups.
29969287	5	70	dep	diets	665:669	arg1	diets					706:710	five test diets	696:710	five test diets supplemented with quercetin (400 mg/kg), oat hulls (50 g/kg), β-glucan (100 mg/kg), lysozyme (40 mg/kg) or fish oil ω-3 fatty acids (40 g/kg), with six replicate pens per treatment	696:891	A total of 1008 one-day-old male Ross 308 chicks were distributed over 36 floor pens and allocated to one of six iso-caloric (AMEN 13 MJ/kg) growing diets (d 15-28): a control and five test diets supplemented with quercetin (400 mg/kg), oat hulls (50 g/kg), β-glucan (100 mg/kg), lysozyme (40 mg/kg) or fish oil ω-3 fatty acids (40 g/kg), with six replicate pens per treatment.
29969287	5	70	dep	diets	665:669	arg1	control					684:690	a control	682:690	a control	682:690	A total of 1008 one-day-old male Ross 308 chicks were distributed over 36 floor pens and allocated to one of six iso-caloric (AMEN 13 MJ/kg) growing diets (d 15-28): a control and five test diets supplemented with quercetin (400 mg/kg), oat hulls (50 g/kg), β-glucan (100 mg/kg), lysozyme (40 mg/kg) or fish oil ω-3 fatty acids (40 g/kg), with six replicate pens per treatment.
29969287	18	71	theme	nutritional	2081:2091	arg1	interventions					2093:2105	the tested nutritional interventions	2070:2105	the tested nutritional interventions in the current experiment	2070:2131	It was concluded that the tested nutritional interventions in the current experiment only marginally effected health status related parameters.
29969287	2	72	theme	feed	238:241	arg1	ingredients					243:253	technical feed ingredients	228:253	technical feed ingredients between 14 and 28 d of age	228:280	An experiment was conducted to evaluate the effects of technical feed ingredients between 14 and 28 d of age on performance and health status of broilers (d 14-35) fed diets with a high inclusion rate of rapeseed meal as a nutritional challenge.
29969287	9	73	theme	microbiota	1087:1096	arg1	diversity					1098:1106	the microbiota diversity	1083:1106	the microbiota diversity in the jejunum and ileum	1083:1131	No effect of interventions on the microbiota diversity in the jejunum and ileum was observed.
29969287	18	74	theme	current	2114:2120	arg1	experiment					2122:2131	the current experiment	2110:2131	the current experiment	2110:2131	It was concluded that the tested nutritional interventions in the current experiment only marginally effected health status related parameters.
29969287	7	75	theme	experimental	1030:1041	arg1	diets					1043:1047	the experimental diets	1026:1047	the experimental diets	1026:1047	Dietary inclusion of oat hulls and lysozyme resulted in a reduction in broiler performance during the first week after providing the experimental diets.
29969287	13	76	dep	area	1548:1551	arg1	d 21					1565:1568	d 21	1565:1568	d 21	1565:1568	Higher total surface area of villi occupied by goblet cells and total villi surface area in jejunum (d 21 and 28) were observed in chickens fed oat hulls compared to other groups.
29969287	13	76	dep	area	1548:1551	arg1	28					1574:1575	28	1574:1575	28	1574:1575	Higher total surface area of villi occupied by goblet cells and total villi surface area in jejunum (d 21 and 28) were observed in chickens fed oat hulls compared to other groups.
29969287	12	77	theme	control	1449:1455	arg1	group					1457:1461	the control group	1445:1461	the control group	1445:1461	In the jejunum, villus height and crypt depth of lysozyme-fed birds at d 28 were decreased compared to the control group.
29969287	5	78	dep	iso-caloric	629:639	arg1	13 MJ/kg					647:654	AMEN 13 MJ/kg	642:654	AMEN 13 MJ/kg	642:654	A total of 1008 one-day-old male Ross 308 chicks were distributed over 36 floor pens and allocated to one of six iso-caloric (AMEN 13 MJ/kg) growing diets (d 15-28): a control and five test diets supplemented with quercetin (400 mg/kg), oat hulls (50 g/kg), β-glucan (100 mg/kg), lysozyme (40 mg/kg) or fish oil ω-3 fatty acids (40 g/kg), with six replicate pens per treatment.
29969287	13	79	theme	surface	1477:1483	arg1	area					1485:1488	Higher total surface area	1464:1488	Higher total surface area of villi occupied by goblet cells	1464:1522	Higher total surface area of villi occupied by goblet cells and total villi surface area in jejunum (d 21 and 28) were observed in chickens fed oat hulls compared to other groups.
29969287	13	80	theme	other	1630:1634	arg1	groups					1636:1641	other groups	1630:1641	other groups	1630:1641	Higher total surface area of villi occupied by goblet cells and total villi surface area in jejunum (d 21 and 28) were observed in chickens fed oat hulls compared to other groups.
29969287	5	81	theme	male	544:547	arg1	chicks					558:563	1008 one-day-old male Ross 308 chicks	527:563	1008 one-day-old male Ross 308 chicks	527:563	A total of 1008 one-day-old male Ross 308 chicks were distributed over 36 floor pens and allocated to one of six iso-caloric (AMEN 13 MJ/kg) growing diets (d 15-28): a control and five test diets supplemented with quercetin (400 mg/kg), oat hulls (50 g/kg), β-glucan (100 mg/kg), lysozyme (40 mg/kg) or fish oil ω-3 fatty acids (40 g/kg), with six replicate pens per treatment.
29969287	15	82	theme	related	1795:1801	arg1	genes					1789:1793	the genes	1785:1793	the genes related to anion-transmembrane-transporter-activity pathway in the quercetin- and oat hull-fed birds	1785:1894	Genes related to the growth-factor-activity pathway were more highly expressed in birds fed β-glucan compared to the control group, while the genes related to anion-transmembrane-transporter-activity pathway in the quercetin- and oat hull-fed birds were less expressed.
29969287	5	83	theme	growing	657:663	arg1	d 15-28					672:678	d 15-28	672:678	d 15-28	672:678	A total of 1008 one-day-old male Ross 308 chicks were distributed over 36 floor pens and allocated to one of six iso-caloric (AMEN 13 MJ/kg) growing diets (d 15-28): a control and five test diets supplemented with quercetin (400 mg/kg), oat hulls (50 g/kg), β-glucan (100 mg/kg), lysozyme (40 mg/kg) or fish oil ω-3 fatty acids (40 g/kg), with six replicate pens per treatment.
29969287	5	83	theme	growing	657:663	arg1	diets					665:669	one of six iso-caloric (AMEN 13 MJ/kg) growing diets	618:669	one of six iso-caloric (AMEN 13 MJ/kg) growing diets (d 15-28): a control and five test diets supplemented with quercetin (400 mg/kg), oat hulls (50 g/kg), β-glucan (100 mg/kg), lysozyme (40 mg/kg) or fish oil ω-3 fatty acids (40 g/kg), with six replicate pens per treatment	618:891	A total of 1008 one-day-old male Ross 308 chicks were distributed over 36 floor pens and allocated to one of six iso-caloric (AMEN 13 MJ/kg) growing diets (d 15-28): a control and five test diets supplemented with quercetin (400 mg/kg), oat hulls (50 g/kg), β-glucan (100 mg/kg), lysozyme (40 mg/kg) or fish oil ω-3 fatty acids (40 g/kg), with six replicate pens per treatment.
29969287	5	84	theme	oil	824:826	arg1	quercetin					730:738	quercetin	730:738	quercetin (400 mg/kg)	730:750	A total of 1008 one-day-old male Ross 308 chicks were distributed over 36 floor pens and allocated to one of six iso-caloric (AMEN 13 MJ/kg) growing diets (d 15-28): a control and five test diets supplemented with quercetin (400 mg/kg), oat hulls (50 g/kg), β-glucan (100 mg/kg), lysozyme (40 mg/kg) or fish oil ω-3 fatty acids (40 g/kg), with six replicate pens per treatment.
29969287	5	84	theme	oil	824:826	arg1	acids					838:842	fish oil ω-3 fatty acids	819:842	fish oil ω-3 fatty acids	819:842	A total of 1008 one-day-old male Ross 308 chicks were distributed over 36 floor pens and allocated to one of six iso-caloric (AMEN 13 MJ/kg) growing diets (d 15-28): a control and five test diets supplemented with quercetin (400 mg/kg), oat hulls (50 g/kg), β-glucan (100 mg/kg), lysozyme (40 mg/kg) or fish oil ω-3 fatty acids (40 g/kg), with six replicate pens per treatment.
29969287	15	85	theme	growth-factor-activity	1668:1689	arg1	pathway					1691:1697	the growth-factor-activity pathway	1664:1697	the growth-factor-activity pathway	1664:1697	Genes related to the growth-factor-activity pathway were more highly expressed in birds fed β-glucan compared to the control group, while the genes related to anion-transmembrane-transporter-activity pathway in the quercetin- and oat hull-fed birds were less expressed.
29969287	13	86	theme	oat	1608:1610	arg1	hulls					1612:1616	oat hulls	1608:1616	oat hulls	1608:1616	Higher total surface area of villi occupied by goblet cells and total villi surface area in jejunum (d 21 and 28) were observed in chickens fed oat hulls compared to other groups.
29969287	5	87	theme	test	701:704	arg1	diets					706:710	five test diets	696:710	five test diets supplemented with quercetin (400 mg/kg), oat hulls (50 g/kg), β-glucan (100 mg/kg), lysozyme (40 mg/kg) or fish oil ω-3 fatty acids (40 g/kg), with six replicate pens per treatment	696:891	A total of 1008 one-day-old male Ross 308 chicks were distributed over 36 floor pens and allocated to one of six iso-caloric (AMEN 13 MJ/kg) growing diets (d 15-28): a control and five test diets supplemented with quercetin (400 mg/kg), oat hulls (50 g/kg), β-glucan (100 mg/kg), lysozyme (40 mg/kg) or fish oil ω-3 fatty acids (40 g/kg), with six replicate pens per treatment.
29969287	2	88	theme	meal	386:389	arg1	rate					369:372	a high inclusion rate	352:372	a high inclusion rate of rapeseed meal	352:389	An experiment was conducted to evaluate the effects of technical feed ingredients between 14 and 28 d of age on performance and health status of broilers (d 14-35) fed diets with a high inclusion rate of rapeseed meal as a nutritional challenge.
29969287	13	89	theme	Higher	1464:1469	arg1	area					1485:1488	Higher total surface area	1464:1488	Higher total surface area of villi occupied by goblet cells	1464:1522	Higher total surface area of villi occupied by goblet cells and total villi surface area in jejunum (d 21 and 28) were observed in chickens fed oat hulls compared to other groups.
29969287	16	90	theme	related	2026:2032	arg1	processes					2034:2042	immune related processes	2019:2042	immune related processes	2019:2042	The genes differently expressed between dietary interventions did not seem to be directly involved in immune related processes.
29969287	0	91	theme	interventions	22:34	arg1	Effect					0:5	Effect	0:5	Effect of nutritional interventions with quercetin, oat hulls, β-glucans, lysozyme and fish oil on performance and health status related parameters of broilers chickens.	0:168	Effect of nutritional interventions with quercetin, oat hulls, β-glucans, lysozyme and fish oil on performance and health status related parameters of broilers chickens.
29969287	15	92	theme	control	1764:1770	arg1	group					1772:1776	the control group	1760:1776	the control group	1760:1776	Genes related to the growth-factor-activity pathway were more highly expressed in birds fed β-glucan compared to the control group, while the genes related to anion-transmembrane-transporter-activity pathway in the quercetin- and oat hull-fed birds were less expressed.
29969287	2	93	from	effects	217:223	arg1	status					308:313	performance and health status	285:313	status	308:313	An experiment was conducted to evaluate the effects of technical feed ingredients between 14 and 28 d of age on performance and health status of broilers (d 14-35) fed diets with a high inclusion rate of rapeseed meal as a nutritional challenge.
29969287	18	94	theme	tested	2074:2079	arg1	interventions					2093:2105	the tested nutritional interventions	2070:2105	the tested nutritional interventions in the current experiment	2070:2131	It was concluded that the tested nutritional interventions in the current experiment only marginally effected health status related parameters.
29969287	2	95	theme	inclusion	359:367	arg1	rate					369:372	a high inclusion rate	352:372	a high inclusion rate of rapeseed meal	352:389	An experiment was conducted to evaluate the effects of technical feed ingredients between 14 and 28 d of age on performance and health status of broilers (d 14-35) fed diets with a high inclusion rate of rapeseed meal as a nutritional challenge.
29969287	15	96	theme	oat	1877:1879	arg1	birds					1890:1894	the quercetin- and oat hull-fed birds	1858:1894	the quercetin- and oat hull-fed birds	1858:1894	Genes related to the growth-factor-activity pathway were more highly expressed in birds fed β-glucan compared to the control group, while the genes related to anion-transmembrane-transporter-activity pathway in the quercetin- and oat hull-fed birds were less expressed.
29969287	10	97	theme	Enterococcus	1275:1286	arg1	abundance					1252:1260	a higher abundance	1243:1260	a higher abundance of the genus Enterococcus	1243:1286	Ileal microbiota composition of birds fed oat hulls differed from the other groups, as shown by a higher abundance of the genus Enterococcus, mainly at the expense of the genus Lactobacillus.
29969287	0	98	with	interventions	22:34	arg1	β-glucans					63:71	β-glucans	63:71	β-glucans	63:71	Effect of nutritional interventions with quercetin, oat hulls, β-glucans, lysozyme and fish oil on performance and health status related parameters of broilers chickens.
29969287	0	98	with	interventions	22:34	arg1	quercetin					41:49	quercetin	41:49	quercetin	41:49	Effect of nutritional interventions with quercetin, oat hulls, β-glucans, lysozyme and fish oil on performance and health status related parameters of broilers chickens.
29969287	0	98	with	interventions	22:34	arg1	hulls					56:60	oat hulls	52:60	oat hulls	52:60	Effect of nutritional interventions with quercetin, oat hulls, β-glucans, lysozyme and fish oil on performance and health status related parameters of broilers chickens.
29969287	0	98	with	interventions	22:34	arg1	lysozyme					74:81	lysozyme	74:81	lysozyme	74:81	Effect of nutritional interventions with quercetin, oat hulls, β-glucans, lysozyme and fish oil on performance and health status related parameters of broilers chickens.
29969287	0	98	with	interventions	22:34	arg1	oil					92:94	fish oil	87:94	fish oil	87:94	Effect of nutritional interventions with quercetin, oat hulls, β-glucans, lysozyme and fish oil on performance and health status related parameters of broilers chickens.
29969287	0	99	theme	oat	52:54	arg1	hulls					56:60	oat hulls	52:60	oat hulls	52:60	Effect of nutritional interventions with quercetin, oat hulls, β-glucans, lysozyme and fish oil on performance and health status related parameters of broilers chickens.
29969287	15	100	theme	quercetin-	1862:1871	arg1	birds					1890:1894	the quercetin- and oat hull-fed birds	1858:1894	the quercetin- and oat hull-fed birds	1858:1894	Genes related to the growth-factor-activity pathway were more highly expressed in birds fed β-glucan compared to the control group, while the genes related to anion-transmembrane-transporter-activity pathway in the quercetin- and oat hull-fed birds were less expressed.
29969287	10	101	theme	Ileal	1147:1151	arg1	composition					1164:1174	Ileal microbiota composition	1147:1174	Ileal microbiota composition of birds fed oat hulls	1147:1197	Ileal microbiota composition of birds fed oat hulls differed from the other groups, as shown by a higher abundance of the genus Enterococcus, mainly at the expense of the genus Lactobacillus.
29969287	2	102	theme	health	301:306	arg1	status					308:313	performance and health status	285:313	status	308:313	An experiment was conducted to evaluate the effects of technical feed ingredients between 14 and 28 d of age on performance and health status of broilers (d 14-35) fed diets with a high inclusion rate of rapeseed meal as a nutritional challenge.
29969287	5	103	theme	fatty	832:836	arg1	quercetin					730:738	quercetin	730:738	quercetin (400 mg/kg)	730:750	A total of 1008 one-day-old male Ross 308 chicks were distributed over 36 floor pens and allocated to one of six iso-caloric (AMEN 13 MJ/kg) growing diets (d 15-28): a control and five test diets supplemented with quercetin (400 mg/kg), oat hulls (50 g/kg), β-glucan (100 mg/kg), lysozyme (40 mg/kg) or fish oil ω-3 fatty acids (40 g/kg), with six replicate pens per treatment.
29969287	5	103	theme	fatty	832:836	arg1	acids					838:842	fish oil ω-3 fatty acids	819:842	fish oil ω-3 fatty acids	819:842	A total of 1008 one-day-old male Ross 308 chicks were distributed over 36 floor pens and allocated to one of six iso-caloric (AMEN 13 MJ/kg) growing diets (d 15-28): a control and five test diets supplemented with quercetin (400 mg/kg), oat hulls (50 g/kg), β-glucan (100 mg/kg), lysozyme (40 mg/kg) or fish oil ω-3 fatty acids (40 g/kg), with six replicate pens per treatment.
29969287	10	104	theme	oat	1189:1191	arg1	hulls					1193:1197	oat hulls	1189:1197	oat hulls	1189:1197	Ileal microbiota composition of birds fed oat hulls differed from the other groups, as shown by a higher abundance of the genus Enterococcus, mainly at the expense of the genus Lactobacillus.
29969287	2	105	theme	performance	285:295	arg1	status					308:313	performance and health status	285:313	status	308:313	An experiment was conducted to evaluate the effects of technical feed ingredients between 14 and 28 d of age on performance and health status of broilers (d 14-35) fed diets with a high inclusion rate of rapeseed meal as a nutritional challenge.
29969287	15	106	theme	anion-transmembrane-transporter-activity	1806:1845	arg1	pathway					1847:1853	anion-transmembrane-transporter-activity pathway	1806:1853	anion-transmembrane-transporter-activity pathway in the quercetin- and oat hull-fed birds	1806:1894	Genes related to the growth-factor-activity pathway were more highly expressed in birds fed β-glucan compared to the control group, while the genes related to anion-transmembrane-transporter-activity pathway in the quercetin- and oat hull-fed birds were less expressed.
29969287	10	107	theme	birds	1179:1183	arg1	composition					1164:1174	Ileal microbiota composition	1147:1174	Ileal microbiota composition of birds fed oat hulls	1147:1197	Ileal microbiota composition of birds fed oat hulls differed from the other groups, as shown by a higher abundance of the genus Enterococcus, mainly at the expense of the genus Lactobacillus.
31367158	8	0	theme	repair	1029:1034	arg1	processes					1036:1044	epithelial repair processes	1018:1044	epithelial repair processes	1018:1044	The colonic luminal environment and epithelial repair processes during the inflammatory flare and colitis resolution were analyzed with reference to a non-DSS treated control group, euthanized at day 0.
31367158	12	1	theme	short-chain	1965:1975	arg1	content					1988:1994	short-chain fatty acid content	1965:1994	short-chain fatty acid content	1965:1994	Although signs of colitis were severe (inflammatory cell infiltrate, crypt disarray, increased permeability) and associated with colonic luminal alterations (hyperosmolarity, dysbiosis, decrease in short-chain fatty acid content), epithelial healing processes were launched early during the inflammatory flare with increased gene expression of certain key epithelial repair modulators, including transforming growth factor-β, interleukin (Il)-15, Il-22, Il-33, and serum amyloid A. Whereas signs of inflammation progressively diminished, luminal colonic environment alterations and microscopic abnormalities of colon mucosa persisted long after colitis induction.
31367158	4	2	theme	luminal	574:580	arg1	characteristics					590:604	the luminal content characteristics	570:604	the luminal content characteristics during this process	570:624	However, the sequence of events associated with colon mucosal repair after colitis and the evolution of the luminal content characteristics during this process have been little studied.
31367158	6	3	theme	%	831:831	arg1	DSS					857:859	3.5% dextran sodium sulfate (DSS)	828:860	3.5% dextran sodium sulfate (DSS) in drinking water for 5 d	828:886	METHODS C57BL/6 male mice were given 3.5% dextran sodium sulfate (DSS) in drinking water for 5 d.
31367158	4	4	theme	characteristics	590:604	arg1	sequence					479:486	the sequence	475:486	the sequence of events associated with colon mucosal repair after colitis	475:547	However, the sequence of events associated with colon mucosal repair after colitis and the evolution of the luminal content characteristics during this process have been little studied.
31367158	4	4	theme	characteristics	590:604	arg1	evolution					557:565	the evolution	553:565	the evolution of the luminal content characteristics during this process	553:624	However, the sequence of events associated with colon mucosal repair after colitis and the evolution of the luminal content characteristics during this process have been little studied.
31367158	10	5	theme	maximal	1500:1506	arg1	alterations					1508:1518	maximal alterations	1500:1518	maximal alterations of intestinal barrier function and histological damage associated with goblet cell depletion in colon mucosa	1500:1627	RESULTS The maximal intensity of colitis was concomitant with maximal alterations of intestinal barrier function and histological damage associated with goblet cell depletion in colon mucosa.
31367158	9	6	theme	factors	1342:1348	arg1	evaluation					1355:1364	evaluation	1355:1364	evaluation of colon mucosa-adherent microbiota composition	1355:1412	Epithelial repair events were assessed histo-morphologically in combination with functional permeability tests, expression of key inflammatory and repairing factors, and evaluation of colon mucosa-adherent microbiota composition by 16S rRNA sequencing.
31367158	9	6	theme	factors	1342:1348	arg1	expression					1297:1306	expression	1297:1306	expression of key inflammatory and repairing factors	1297:1348	Epithelial repair events were assessed histo-morphologically in combination with functional permeability tests, expression of key inflammatory and repairing factors, and evaluation of colon mucosa-adherent microbiota composition by 16S rRNA sequencing.
31367158	9	6	theme	factors	1342:1348	arg1	tests					1290:1294	functional permeability tests	1266:1294	functional permeability tests	1266:1294	Epithelial repair events were assessed histo-morphologically in combination with functional permeability tests, expression of key inflammatory and repairing factors, and evaluation of colon mucosa-adherent microbiota composition by 16S rRNA sequencing.
31367158	8	7	theme	inflammatory	1057:1068	arg1	flare					1070:1074	the inflammatory flare	1053:1074	the inflammatory flare	1053:1074	The colonic luminal environment and epithelial repair processes during the inflammatory flare and colitis resolution were analyzed with reference to a non-DSS treated control group, euthanized at day 0.
31367158	3	8	theme	wide	422:425	arg1	range					427:431	a wide range	420:431	a wide range of distinct regulatory proteins	420:463	These events are longitudinally and tightly controlled by numerous factors including a wide range of distinct regulatory proteins.
31367158	12	9	theme	inflammatory	2058:2069	arg1	flare					2071:2075	the inflammatory flare	2054:2075	the inflammatory flare with increased gene expression of certain key epithelial repair modulators	2054:2150	Although signs of colitis were severe (inflammatory cell infiltrate, crypt disarray, increased permeability) and associated with colonic luminal alterations (hyperosmolarity, dysbiosis, decrease in short-chain fatty acid content), epithelial healing processes were launched early during the inflammatory flare with increased gene expression of certain key epithelial repair modulators, including transforming growth factor-β, interleukin (Il)-15, Il-22, Il-33, and serum amyloid A. Whereas signs of inflammation progressively diminished, luminal colonic environment alterations and microscopic abnormalities of colon mucosa persisted long after colitis induction.
31367158	11	10	dep	d	1648:1648	arg1	termination					1656:1666	termination	1656:1666	termination of the DSS-treatment	1656:1687	It was recorded 2 d after termination of the DSS-treatment, followed by a progressive return to values similar to those of control mice.
31367158	1	11	theme	bowel	172:176	arg1	diseases					178:185	inflammatory bowel diseases	159:185	inflammatory bowel diseases	159:185	BACKGROUND Mucosal healing has become a therapeutic goal to achieve stable remission in patients with inflammatory bowel diseases.
31367158	12	12	theme	colonic	1896:1902	arg1	alterations					1912:1922	colonic luminal alterations	1896:1922	colonic luminal alterations (hyperosmolarity, dysbiosis, decrease in short-chain fatty acid content)	1896:1995	Although signs of colitis were severe (inflammatory cell infiltrate, crypt disarray, increased permeability) and associated with colonic luminal alterations (hyperosmolarity, dysbiosis, decrease in short-chain fatty acid content), epithelial healing processes were launched early during the inflammatory flare with increased gene expression of certain key epithelial repair modulators, including transforming growth factor-β, interleukin (Il)-15, Il-22, Il-33, and serum amyloid A. Whereas signs of inflammation progressively diminished, luminal colonic environment alterations and microscopic abnormalities of colon mucosa persisted long after colitis induction.
31367158	10	13	with	concomitant	1483:1493	arg1	alterations					1508:1518	maximal alterations	1500:1518	maximal alterations of intestinal barrier function and histological damage associated with goblet cell depletion in colon mucosa	1500:1627	RESULTS The maximal intensity of colitis was concomitant with maximal alterations of intestinal barrier function and histological damage associated with goblet cell depletion in colon mucosa.
31367158	8	14	theme	treated	1141:1147	arg1	group					1157:1161	a non-DSS treated control group	1131:1161	a non-DSS treated control group	1131:1161	The colonic luminal environment and epithelial repair processes during the inflammatory flare and colitis resolution were analyzed with reference to a non-DSS treated control group, euthanized at day 0.
31367158	10	15	theme	barrier	1534:1540	arg1	function					1542:1549	intestinal barrier function	1523:1549	intestinal barrier function	1523:1549	RESULTS The maximal intensity of colitis was concomitant with maximal alterations of intestinal barrier function and histological damage associated with goblet cell depletion in colon mucosa.
31367158	11	16	theme	similar	1733:1739	arg1	values					1726:1731	values	1726:1731	values similar to those of control mice	1726:1764	It was recorded 2 d after termination of the DSS-treatment, followed by a progressive return to values similar to those of control mice.
31367158	6	17	theme	C57BL/6	799:805	arg1	mice					812:815	METHODS C57BL/6 male mice	791:815	METHODS C57BL/6 male mice	791:815	METHODS C57BL/6 male mice were given 3.5% dextran sodium sulfate (DSS) in drinking water for 5 d.
31367158	13	18	theme	modulators	2634:2643	arg1	involvement					2615:2625	the longitudinal involvement	2598:2625	the longitudinal involvement of key modulators	2598:2643	CONCLUSION This study shows that colon repair can be initiated in the context of inflamed mucosa associated with alterations of the luminal environment and highlights the longitudinal involvement of key modulators.
31367158	9	19	theme	mucosa-adherent	1375:1389	arg1	composition					1402:1412	colon mucosa-adherent microbiota composition	1369:1412	colon mucosa-adherent microbiota composition	1369:1412	Epithelial repair events were assessed histo-morphologically in combination with functional permeability tests, expression of key inflammatory and repairing factors, and evaluation of colon mucosa-adherent microbiota composition by 16S rRNA sequencing.
31367158	10	20	theme	damage	1568:1573	arg1	alterations					1508:1518	maximal alterations	1500:1518	maximal alterations of intestinal barrier function and histological damage associated with goblet cell depletion in colon mucosa	1500:1627	RESULTS The maximal intensity of colitis was concomitant with maximal alterations of intestinal barrier function and histological damage associated with goblet cell depletion in colon mucosa.
31367158	11	21	theme	control	1753:1759	arg1	mice					1761:1764	control mice	1753:1764	control mice	1753:1764	It was recorded 2 d after termination of the DSS-treatment, followed by a progressive return to values similar to those of control mice.
31367158	9	22	theme	composition	1402:1412	arg1	evaluation					1355:1364	evaluation	1355:1364	evaluation of colon mucosa-adherent microbiota composition	1355:1412	Epithelial repair events were assessed histo-morphologically in combination with functional permeability tests, expression of key inflammatory and repairing factors, and evaluation of colon mucosa-adherent microbiota composition by 16S rRNA sequencing.
31367158	9	22	theme	composition	1402:1412	arg1	expression					1297:1306	expression	1297:1306	expression of key inflammatory and repairing factors	1297:1348	Epithelial repair events were assessed histo-morphologically in combination with functional permeability tests, expression of key inflammatory and repairing factors, and evaluation of colon mucosa-adherent microbiota composition by 16S rRNA sequencing.
31367158	9	22	theme	composition	1402:1412	arg1	tests					1290:1294	functional permeability tests	1266:1294	functional permeability tests	1266:1294	Epithelial repair events were assessed histo-morphologically in combination with functional permeability tests, expression of key inflammatory and repairing factors, and evaluation of colon mucosa-adherent microbiota composition by 16S rRNA sequencing.
31367158	12	23	theme	epithelial	1998:2007	arg1	processes					2017:2025	epithelial healing processes	1998:2025	epithelial healing processes	1998:2025	Although signs of colitis were severe (inflammatory cell infiltrate, crypt disarray, increased permeability) and associated with colonic luminal alterations (hyperosmolarity, dysbiosis, decrease in short-chain fatty acid content), epithelial healing processes were launched early during the inflammatory flare with increased gene expression of certain key epithelial repair modulators, including transforming growth factor-β, interleukin (Il)-15, Il-22, Il-33, and serum amyloid A. Whereas signs of inflammation progressively diminished, luminal colonic environment alterations and microscopic abnormalities of colon mucosa persisted long after colitis induction.
31367158	7	24	theme	23	949:950	arg1	d					961:961	2 (day 7), 5 (day 10), 8 (day 13), and 23 (day 28) d	910:961	2 (day 7), 5 (day 10), 8 (day 13), and 23 (day 28) d	910:961	They were euthanized 2 (day 7), 5 (day 10), 8 (day 13), and 23 (day 28) d after DSS removal.
31367158	7	25	theme	8	933:933	arg1	d					961:961	2 (day 7), 5 (day 10), 8 (day 13), and 23 (day 28) d	910:961	2 (day 7), 5 (day 10), 8 (day 13), and 23 (day 28) d	910:961	They were euthanized 2 (day 7), 5 (day 10), 8 (day 13), and 23 (day 28) d after DSS removal.
31367158	9	26	theme	16S	1417:1419	arg1	sequencing					1426:1435	16S rRNA sequencing	1417:1435	16S rRNA sequencing	1417:1435	Epithelial repair events were assessed histo-morphologically in combination with functional permeability tests, expression of key inflammatory and repairing factors, and evaluation of colon mucosa-adherent microbiota composition by 16S rRNA sequencing.
31367158	5	27	theme	mucosal	691:697	arg1	characteristics					699:713	colon mucosal characteristics	685:713	colon mucosal characteristics	685:713	AIM To document the evolution of colon mucosal characteristics during mucosal healing using a mouse model with chemically-induced colitis.
31367158	2	28	theme	processes	255:263	arg1	actions					227:233	overlapping actions	215:233	overlapping actions of complex cellular processes, such as migration, proliferation, and differentiation,	215:319	To achieve this objective, overlapping actions of complex cellular processes, such as migration, proliferation, and differentiation, are required.
31367158	1	29	theme	stable	125:130	arg1	remission					132:140	stable remission	125:140	stable remission in patients with inflammatory bowel diseases	125:185	BACKGROUND Mucosal healing has become a therapeutic goal to achieve stable remission in patients with inflammatory bowel diseases.
31367158	12	30	theme	serum	2232:2236	arg1	amyloid					2238:2244	serum amyloid	2232:2244	serum amyloid	2232:2244	Although signs of colitis were severe (inflammatory cell infiltrate, crypt disarray, increased permeability) and associated with colonic luminal alterations (hyperosmolarity, dysbiosis, decrease in short-chain fatty acid content), epithelial healing processes were launched early during the inflammatory flare with increased gene expression of certain key epithelial repair modulators, including transforming growth factor-β, interleukin (Il)-15, Il-22, Il-33, and serum amyloid A. Whereas signs of inflammation progressively diminished, luminal colonic environment alterations and microscopic abnormalities of colon mucosa persisted long after colitis induction.
31367158	12	31	theme	acid	1983:1986	arg1	content					1988:1994	short-chain fatty acid content	1965:1994	short-chain fatty acid content	1965:1994	Although signs of colitis were severe (inflammatory cell infiltrate, crypt disarray, increased permeability) and associated with colonic luminal alterations (hyperosmolarity, dysbiosis, decrease in short-chain fatty acid content), epithelial healing processes were launched early during the inflammatory flare with increased gene expression of certain key epithelial repair modulators, including transforming growth factor-β, interleukin (Il)-15, Il-22, Il-33, and serum amyloid A. Whereas signs of inflammation progressively diminished, luminal colonic environment alterations and microscopic abnormalities of colon mucosa persisted long after colitis induction.
31367158	5	32	theme	chemically-induced	763:780	arg1	colitis					782:788	chemically-induced colitis	763:788	chemically-induced colitis	763:788	AIM To document the evolution of colon mucosal characteristics during mucosal healing using a mouse model with chemically-induced colitis.
31367158	2	33	theme	complex	238:244	arg1	migration					274:282	migration	274:282	migration	274:282	To achieve this objective, overlapping actions of complex cellular processes, such as migration, proliferation, and differentiation, are required.
31367158	2	33	theme	complex	238:244	arg1	processes					255:263	complex cellular processes	238:263	complex cellular processes	238:263	To achieve this objective, overlapping actions of complex cellular processes, such as migration, proliferation, and differentiation, are required.
31367158	2	33	theme	complex	238:244	arg1	proliferation					285:297	proliferation	285:297	proliferation	285:297	To achieve this objective, overlapping actions of complex cellular processes, such as migration, proliferation, and differentiation, are required.
31367158	2	33	theme	complex	238:244	arg1	differentiation					304:318	differentiation	304:318	differentiation	304:318	To achieve this objective, overlapping actions of complex cellular processes, such as migration, proliferation, and differentiation, are required.
31367158	13	34	dep	CONCLUSION	2431:2440	arg1	highlights					2587:2596	highlights	2587:2596	highlights the longitudinal involvement of key modulators	2587:2643	CONCLUSION This study shows that colon repair can be initiated in the context of inflamed mucosa associated with alterations of the luminal environment and highlights the longitudinal involvement of key modulators.
31367158	13	34	dep	CONCLUSION	2431:2440	arg1	shows					2453:2457	shows	2453:2457	shows that colon repair can be initiated in the context of inflamed mucosa associated with alterations of the luminal environment	2453:2581	CONCLUSION This study shows that colon repair can be initiated in the context of inflamed mucosa associated with alterations of the luminal environment and highlights the longitudinal involvement of key modulators.
31367158	4	35	theme	events	491:496	arg1	sequence					479:486	the sequence	475:486	the sequence of events associated with colon mucosal repair after colitis	475:547	However, the sequence of events associated with colon mucosal repair after colitis and the evolution of the luminal content characteristics during this process have been little studied.
31367158	4	35	theme	events	491:496	arg1	evolution					557:565	the evolution	553:565	the evolution of the luminal content characteristics during this process	553:624	However, the sequence of events associated with colon mucosal repair after colitis and the evolution of the luminal content characteristics during this process have been little studied.
31367158	11	36	theme	progressive	1704:1714	arg1	return					1716:1721	a progressive return	1702:1721	a progressive return to values similar to those of control mice	1702:1764	It was recorded 2 d after termination of the DSS-treatment, followed by a progressive return to values similar to those of control mice.
31367158	12	37	theme	crypt	1836:1840	arg1	infiltrate					1824:1833	inflammatory cell infiltrate	1806:1833	inflammatory cell infiltrate	1806:1833	Although signs of colitis were severe (inflammatory cell infiltrate, crypt disarray, increased permeability) and associated with colonic luminal alterations (hyperosmolarity, dysbiosis, decrease in short-chain fatty acid content), epithelial healing processes were launched early during the inflammatory flare with increased gene expression of certain key epithelial repair modulators, including transforming growth factor-β, interleukin (Il)-15, Il-22, Il-33, and serum amyloid A. Whereas signs of inflammation progressively diminished, luminal colonic environment alterations and microscopic abnormalities of colon mucosa persisted long after colitis induction.
31367158	12	37	theme	crypt	1836:1840	arg1	disarray					1842:1849	crypt disarray	1836:1849	crypt disarray	1836:1849	Although signs of colitis were severe (inflammatory cell infiltrate, crypt disarray, increased permeability) and associated with colonic luminal alterations (hyperosmolarity, dysbiosis, decrease in short-chain fatty acid content), epithelial healing processes were launched early during the inflammatory flare with increased gene expression of certain key epithelial repair modulators, including transforming growth factor-β, interleukin (Il)-15, Il-22, Il-33, and serum amyloid A. Whereas signs of inflammation progressively diminished, luminal colonic environment alterations and microscopic abnormalities of colon mucosa persisted long after colitis induction.
31367158	7	38	theme	5	921:921	arg1	d					961:961	2 (day 7), 5 (day 10), 8 (day 13), and 23 (day 28) d	910:961	2 (day 7), 5 (day 10), 8 (day 13), and 23 (day 28) d	910:961	They were euthanized 2 (day 7), 5 (day 10), 8 (day 13), and 23 (day 28) d after DSS removal.
31367158	13	39	theme	luminal	2563:2569	arg1	environment					2571:2581	the luminal environment	2559:2581	the luminal environment	2559:2581	CONCLUSION This study shows that colon repair can be initiated in the context of inflamed mucosa associated with alterations of the luminal environment and highlights the longitudinal involvement of key modulators.
31367158	9	40	theme	Epithelial	1185:1194	arg1	events					1203:1208	Epithelial repair events	1185:1208	Epithelial repair events	1185:1208	Epithelial repair events were assessed histo-morphologically in combination with functional permeability tests, expression of key inflammatory and repairing factors, and evaluation of colon mucosa-adherent microbiota composition by 16S rRNA sequencing.
31367158	1	41	from	remission	132:140	arg1	patients					145:152	patients	145:152	patients with inflammatory bowel diseases	145:185	BACKGROUND Mucosal healing has become a therapeutic goal to achieve stable remission in patients with inflammatory bowel diseases.
31367158	1	42	theme	BACKGROUND	57:66	arg1	healing					76:82	BACKGROUND Mucosal healing	57:82	BACKGROUND Mucosal healing	57:82	BACKGROUND Mucosal healing has become a therapeutic goal to achieve stable remission in patients with inflammatory bowel diseases.
31367158	12	43	dep	alterations	1912:1922	arg1	decrease					1953:1960	decrease	1953:1960	decrease in short-chain fatty acid content	1953:1994	Although signs of colitis were severe (inflammatory cell infiltrate, crypt disarray, increased permeability) and associated with colonic luminal alterations (hyperosmolarity, dysbiosis, decrease in short-chain fatty acid content), epithelial healing processes were launched early during the inflammatory flare with increased gene expression of certain key epithelial repair modulators, including transforming growth factor-β, interleukin (Il)-15, Il-22, Il-33, and serum amyloid A. Whereas signs of inflammation progressively diminished, luminal colonic environment alterations and microscopic abnormalities of colon mucosa persisted long after colitis induction.
31367158	12	43	dep	alterations	1912:1922	arg1	dysbiosis					1942:1950	dysbiosis	1942:1950	dysbiosis	1942:1950	Although signs of colitis were severe (inflammatory cell infiltrate, crypt disarray, increased permeability) and associated with colonic luminal alterations (hyperosmolarity, dysbiosis, decrease in short-chain fatty acid content), epithelial healing processes were launched early during the inflammatory flare with increased gene expression of certain key epithelial repair modulators, including transforming growth factor-β, interleukin (Il)-15, Il-22, Il-33, and serum amyloid A. Whereas signs of inflammation progressively diminished, luminal colonic environment alterations and microscopic abnormalities of colon mucosa persisted long after colitis induction.
31367158	12	43	dep	alterations	1912:1922	arg1	hyperosmolarity					1925:1939	hyperosmolarity	1925:1939	hyperosmolarity	1925:1939	Although signs of colitis were severe (inflammatory cell infiltrate, crypt disarray, increased permeability) and associated with colonic luminal alterations (hyperosmolarity, dysbiosis, decrease in short-chain fatty acid content), epithelial healing processes were launched early during the inflammatory flare with increased gene expression of certain key epithelial repair modulators, including transforming growth factor-β, interleukin (Il)-15, Il-22, Il-33, and serum amyloid A. Whereas signs of inflammation progressively diminished, luminal colonic environment alterations and microscopic abnormalities of colon mucosa persisted long after colitis induction.
31367158	12	44	theme	inflammatory	1806:1817	arg1	infiltrate					1824:1833	inflammatory cell infiltrate	1806:1833	inflammatory cell infiltrate	1806:1833	Although signs of colitis were severe (inflammatory cell infiltrate, crypt disarray, increased permeability) and associated with colonic luminal alterations (hyperosmolarity, dysbiosis, decrease in short-chain fatty acid content), epithelial healing processes were launched early during the inflammatory flare with increased gene expression of certain key epithelial repair modulators, including transforming growth factor-β, interleukin (Il)-15, Il-22, Il-33, and serum amyloid A. Whereas signs of inflammation progressively diminished, luminal colonic environment alterations and microscopic abnormalities of colon mucosa persisted long after colitis induction.
31367158	12	44	theme	inflammatory	1806:1817	arg1	disarray					1842:1849	crypt disarray	1836:1849	crypt disarray	1836:1849	Although signs of colitis were severe (inflammatory cell infiltrate, crypt disarray, increased permeability) and associated with colonic luminal alterations (hyperosmolarity, dysbiosis, decrease in short-chain fatty acid content), epithelial healing processes were launched early during the inflammatory flare with increased gene expression of certain key epithelial repair modulators, including transforming growth factor-β, interleukin (Il)-15, Il-22, Il-33, and serum amyloid A. Whereas signs of inflammation progressively diminished, luminal colonic environment alterations and microscopic abnormalities of colon mucosa persisted long after colitis induction.
31367158	12	44	theme	inflammatory	1806:1817	arg1	permeability					1862:1873	increased permeability	1852:1873	increased permeability	1852:1873	Although signs of colitis were severe (inflammatory cell infiltrate, crypt disarray, increased permeability) and associated with colonic luminal alterations (hyperosmolarity, dysbiosis, decrease in short-chain fatty acid content), epithelial healing processes were launched early during the inflammatory flare with increased gene expression of certain key epithelial repair modulators, including transforming growth factor-β, interleukin (Il)-15, Il-22, Il-33, and serum amyloid A. Whereas signs of inflammation progressively diminished, luminal colonic environment alterations and microscopic abnormalities of colon mucosa persisted long after colitis induction.
31367158	10	45	theme	goblet	1591:1596	arg1	depletion					1603:1611	goblet cell depletion	1591:1611	goblet cell depletion in colon mucosa	1591:1627	RESULTS The maximal intensity of colitis was concomitant with maximal alterations of intestinal barrier function and histological damage associated with goblet cell depletion in colon mucosa.
31367158	0	46	theme	Mucosal	0:6	arg1	progression					16:26	Mucosal healing progression	0:26	Mucosal healing progression	0:26	Mucosal healing progression after acute colitis in mice.
31367158	7	47	theme	2	910:910	arg1	d					961:961	2 (day 7), 5 (day 10), 8 (day 13), and 23 (day 28) d	910:961	2 (day 7), 5 (day 10), 8 (day 13), and 23 (day 28) d	910:961	They were euthanized 2 (day 7), 5 (day 10), 8 (day 13), and 23 (day 28) d after DSS removal.
31367158	12	48	theme	colon	2378:2382	arg1	mucosa					2384:2389	colon mucosa	2378:2389	colon mucosa	2378:2389	Although signs of colitis were severe (inflammatory cell infiltrate, crypt disarray, increased permeability) and associated with colonic luminal alterations (hyperosmolarity, dysbiosis, decrease in short-chain fatty acid content), epithelial healing processes were launched early during the inflammatory flare with increased gene expression of certain key epithelial repair modulators, including transforming growth factor-β, interleukin (Il)-15, Il-22, Il-33, and serum amyloid A. Whereas signs of inflammation progressively diminished, luminal colonic environment alterations and microscopic abnormalities of colon mucosa persisted long after colitis induction.
31367158	12	49	theme	transforming	2163:2174	arg1	factor-β					2183:2190	transforming growth factor-β	2163:2190	transforming growth factor-β	2163:2190	Although signs of colitis were severe (inflammatory cell infiltrate, crypt disarray, increased permeability) and associated with colonic luminal alterations (hyperosmolarity, dysbiosis, decrease in short-chain fatty acid content), epithelial healing processes were launched early during the inflammatory flare with increased gene expression of certain key epithelial repair modulators, including transforming growth factor-β, interleukin (Il)-15, Il-22, Il-33, and serum amyloid A. Whereas signs of inflammation progressively diminished, luminal colonic environment alterations and microscopic abnormalities of colon mucosa persisted long after colitis induction.
31367158	12	50	theme	colitis	1785:1791	arg1	signs					1776:1780	signs	1776:1780	signs of colitis	1776:1791	Although signs of colitis were severe (inflammatory cell infiltrate, crypt disarray, increased permeability) and associated with colonic luminal alterations (hyperosmolarity, dysbiosis, decrease in short-chain fatty acid content), epithelial healing processes were launched early during the inflammatory flare with increased gene expression of certain key epithelial repair modulators, including transforming growth factor-β, interleukin (Il)-15, Il-22, Il-33, and serum amyloid A. Whereas signs of inflammation progressively diminished, luminal colonic environment alterations and microscopic abnormalities of colon mucosa persisted long after colitis induction.
31367158	6	51	theme	drinking	865:872	arg1	water					874:878	drinking water	865:878	drinking water	865:878	METHODS C57BL/6 male mice were given 3.5% dextran sodium sulfate (DSS) in drinking water for 5 d.
31367158	1	52	theme	therapeutic	97:107	arg1	goal					109:112	a therapeutic goal	95:112	a therapeutic goal to achieve stable remission in patients with inflammatory bowel diseases	95:185	BACKGROUND Mucosal healing has become a therapeutic goal to achieve stable remission in patients with inflammatory bowel diseases.
31367158	10	53	theme	colon	1616:1620	arg1	mucosa					1622:1627	colon mucosa	1616:1627	colon mucosa	1616:1627	RESULTS The maximal intensity of colitis was concomitant with maximal alterations of intestinal barrier function and histological damage associated with goblet cell depletion in colon mucosa.
31367158	12	54	theme	key	2119:2121	arg1	modulators					2141:2150	certain key epithelial repair modulators	2111:2150	certain key epithelial repair modulators	2111:2150	Although signs of colitis were severe (inflammatory cell infiltrate, crypt disarray, increased permeability) and associated with colonic luminal alterations (hyperosmolarity, dysbiosis, decrease in short-chain fatty acid content), epithelial healing processes were launched early during the inflammatory flare with increased gene expression of certain key epithelial repair modulators, including transforming growth factor-β, interleukin (Il)-15, Il-22, Il-33, and serum amyloid A. Whereas signs of inflammation progressively diminished, luminal colonic environment alterations and microscopic abnormalities of colon mucosa persisted long after colitis induction.
31367158	3	55	theme	regulatory	445:454	arg1	proteins					456:463	distinct regulatory proteins	436:463	distinct regulatory proteins	436:463	These events are longitudinally and tightly controlled by numerous factors including a wide range of distinct regulatory proteins.
31367158	0	56	theme	acute	34:38	arg1	colitis					40:46	acute colitis	34:46	acute colitis in mice	34:54	Mucosal healing progression after acute colitis in mice.
31367158	3	57	theme	numerous	393:400	arg1	factors					402:408	numerous factors	393:408	numerous factors including a wide range of distinct regulatory proteins	393:463	These events are longitudinally and tightly controlled by numerous factors including a wide range of distinct regulatory proteins.
31367158	3	57	theme	numerous	393:400	arg1	range					427:431	a wide range	420:431	a wide range of distinct regulatory proteins	420:463	These events are longitudinally and tightly controlled by numerous factors including a wide range of distinct regulatory proteins.
31367158	4	58	theme	mucosal	520:526	arg1	repair					528:533	colon mucosal repair	514:533	colon mucosal repair after colitis	514:547	However, the sequence of events associated with colon mucosal repair after colitis and the evolution of the luminal content characteristics during this process have been little studied.
31367158	10	59	theme	maximal	1450:1456	arg1	intensity					1458:1466	The maximal intensity	1446:1466	The maximal intensity of colitis	1446:1477	RESULTS The maximal intensity of colitis was concomitant with maximal alterations of intestinal barrier function and histological damage associated with goblet cell depletion in colon mucosa.
31367158	10	59	theme	maximal	1450:1456	arg1	concomitant					1483:1493	concomitant	1483:1493	concomitant	1483:1493	RESULTS The maximal intensity of colitis was concomitant with maximal alterations of intestinal barrier function and histological damage associated with goblet cell depletion in colon mucosa.
31367158	12	60	theme	repair	2134:2139	arg1	modulators					2141:2150	certain key epithelial repair modulators	2111:2150	certain key epithelial repair modulators	2111:2150	Although signs of colitis were severe (inflammatory cell infiltrate, crypt disarray, increased permeability) and associated with colonic luminal alterations (hyperosmolarity, dysbiosis, decrease in short-chain fatty acid content), epithelial healing processes were launched early during the inflammatory flare with increased gene expression of certain key epithelial repair modulators, including transforming growth factor-β, interleukin (Il)-15, Il-22, Il-33, and serum amyloid A. Whereas signs of inflammation progressively diminished, luminal colonic environment alterations and microscopic abnormalities of colon mucosa persisted long after colitis induction.
31367158	12	61	with	flare	2071:2075	arg1	expression					2097:2106	increased gene expression	2082:2106	increased gene expression of certain key epithelial repair modulators	2082:2150	Although signs of colitis were severe (inflammatory cell infiltrate, crypt disarray, increased permeability) and associated with colonic luminal alterations (hyperosmolarity, dysbiosis, decrease in short-chain fatty acid content), epithelial healing processes were launched early during the inflammatory flare with increased gene expression of certain key epithelial repair modulators, including transforming growth factor-β, interleukin (Il)-15, Il-22, Il-33, and serum amyloid A. Whereas signs of inflammation progressively diminished, luminal colonic environment alterations and microscopic abnormalities of colon mucosa persisted long after colitis induction.
31367158	13	62	theme	mucosa	2521:2526	arg1	context					2501:2507	the context	2497:2507	the context of inflamed mucosa associated with alterations of the luminal environment	2497:2581	CONCLUSION This study shows that colon repair can be initiated in the context of inflamed mucosa associated with alterations of the luminal environment and highlights the longitudinal involvement of key modulators.
31367158	9	63	theme	permeability	1277:1288	arg1	tests					1290:1294	functional permeability tests	1266:1294	functional permeability tests	1266:1294	Epithelial repair events were assessed histo-morphologically in combination with functional permeability tests, expression of key inflammatory and repairing factors, and evaluation of colon mucosa-adherent microbiota composition by 16S rRNA sequencing.
31367158	13	64	theme	colon	2464:2468	arg1	repair					2470:2475	colon repair	2464:2475	colon repair	2464:2475	CONCLUSION This study shows that colon repair can be initiated in the context of inflamed mucosa associated with alterations of the luminal environment and highlights the longitudinal involvement of key modulators.
31367158	10	65	from	depletion	1603:1611	arg1	mucosa					1622:1627	colon mucosa	1616:1627	colon mucosa	1616:1627	RESULTS The maximal intensity of colitis was concomitant with maximal alterations of intestinal barrier function and histological damage associated with goblet cell depletion in colon mucosa.
31367158	12	66	theme	gene	2092:2095	arg1	expression					2097:2106	increased gene expression	2082:2106	increased gene expression of certain key epithelial repair modulators	2082:2150	Although signs of colitis were severe (inflammatory cell infiltrate, crypt disarray, increased permeability) and associated with colonic luminal alterations (hyperosmolarity, dysbiosis, decrease in short-chain fatty acid content), epithelial healing processes were launched early during the inflammatory flare with increased gene expression of certain key epithelial repair modulators, including transforming growth factor-β, interleukin (Il)-15, Il-22, Il-33, and serum amyloid A. Whereas signs of inflammation progressively diminished, luminal colonic environment alterations and microscopic abnormalities of colon mucosa persisted long after colitis induction.
31367158	8	67	theme	colonic	986:992	arg1	environment					1002:1012	colonic luminal environment	986:1012	colonic luminal environment	986:1012	The colonic luminal environment and epithelial repair processes during the inflammatory flare and colitis resolution were analyzed with reference to a non-DSS treated control group, euthanized at day 0.
31367158	6	68	theme	sodium	841:846	arg1	DSS					857:859	3.5% dextran sodium sulfate (DSS)	828:860	3.5% dextran sodium sulfate (DSS) in drinking water for 5 d	828:886	METHODS C57BL/6 male mice were given 3.5% dextran sodium sulfate (DSS) in drinking water for 5 d.
31367158	0	69	dep	progression	16:26	arg1	colitis					40:46	acute colitis	34:46	acute colitis in mice	34:54	Mucosal healing progression after acute colitis in mice.
31367158	12	70	theme	luminal	2305:2311	arg1	environment					2321:2331	luminal colonic environment	2305:2331	luminal colonic environment alterations	2305:2343	Although signs of colitis were severe (inflammatory cell infiltrate, crypt disarray, increased permeability) and associated with colonic luminal alterations (hyperosmolarity, dysbiosis, decrease in short-chain fatty acid content), epithelial healing processes were launched early during the inflammatory flare with increased gene expression of certain key epithelial repair modulators, including transforming growth factor-β, interleukin (Il)-15, Il-22, Il-33, and serum amyloid A. Whereas signs of inflammation progressively diminished, luminal colonic environment alterations and microscopic abnormalities of colon mucosa persisted long after colitis induction.
31367158	11	71	dep	recorded	1637:1644	arg1	followed					1690:1697	followed	1690:1697	followed by a progressive return to values similar to those of control mice	1690:1764	It was recorded 2 d after termination of the DSS-treatment, followed by a progressive return to values similar to those of control mice.
31367158	12	72	theme	environment	2321:2331	arg1	alterations					2333:2343	luminal colonic environment alterations	2305:2343	luminal colonic environment alterations	2305:2343	Although signs of colitis were severe (inflammatory cell infiltrate, crypt disarray, increased permeability) and associated with colonic luminal alterations (hyperosmolarity, dysbiosis, decrease in short-chain fatty acid content), epithelial healing processes were launched early during the inflammatory flare with increased gene expression of certain key epithelial repair modulators, including transforming growth factor-β, interleukin (Il)-15, Il-22, Il-33, and serum amyloid A. Whereas signs of inflammation progressively diminished, luminal colonic environment alterations and microscopic abnormalities of colon mucosa persisted long after colitis induction.
31367158	9	73	theme	inflammatory	1315:1326	arg1	factors					1342:1348	key inflammatory and repairing factors	1311:1348	key inflammatory and repairing factors	1311:1348	Epithelial repair events were assessed histo-morphologically in combination with functional permeability tests, expression of key inflammatory and repairing factors, and evaluation of colon mucosa-adherent microbiota composition by 16S rRNA sequencing.
31367158	8	74	theme	epithelial	1018:1027	arg1	processes					1036:1044	epithelial repair processes	1018:1044	epithelial repair processes	1018:1044	The colonic luminal environment and epithelial repair processes during the inflammatory flare and colitis resolution were analyzed with reference to a non-DSS treated control group, euthanized at day 0.
31367158	12	75	from	decrease	1953:1960	arg1	content					1988:1994	short-chain fatty acid content	1965:1994	short-chain fatty acid content	1965:1994	Although signs of colitis were severe (inflammatory cell infiltrate, crypt disarray, increased permeability) and associated with colonic luminal alterations (hyperosmolarity, dysbiosis, decrease in short-chain fatty acid content), epithelial healing processes were launched early during the inflammatory flare with increased gene expression of certain key epithelial repair modulators, including transforming growth factor-β, interleukin (Il)-15, Il-22, Il-33, and serum amyloid A. Whereas signs of inflammation progressively diminished, luminal colonic environment alterations and microscopic abnormalities of colon mucosa persisted long after colitis induction.
31367158	12	76	theme	inflammation	2266:2277	arg1	signs					2257:2261	signs	2257:2261	signs of inflammation	2257:2277	Although signs of colitis were severe (inflammatory cell infiltrate, crypt disarray, increased permeability) and associated with colonic luminal alterations (hyperosmolarity, dysbiosis, decrease in short-chain fatty acid content), epithelial healing processes were launched early during the inflammatory flare with increased gene expression of certain key epithelial repair modulators, including transforming growth factor-β, interleukin (Il)-15, Il-22, Il-33, and serum amyloid A. Whereas signs of inflammation progressively diminished, luminal colonic environment alterations and microscopic abnormalities of colon mucosa persisted long after colitis induction.
31367158	9	77	with	combination	1249:1259	arg1	evaluation					1355:1364	evaluation	1355:1364	evaluation of colon mucosa-adherent microbiota composition	1355:1412	Epithelial repair events were assessed histo-morphologically in combination with functional permeability tests, expression of key inflammatory and repairing factors, and evaluation of colon mucosa-adherent microbiota composition by 16S rRNA sequencing.
31367158	9	77	with	combination	1249:1259	arg1	expression					1297:1306	expression	1297:1306	expression of key inflammatory and repairing factors	1297:1348	Epithelial repair events were assessed histo-morphologically in combination with functional permeability tests, expression of key inflammatory and repairing factors, and evaluation of colon mucosa-adherent microbiota composition by 16S rRNA sequencing.
31367158	9	77	with	combination	1249:1259	arg1	tests					1290:1294	functional permeability tests	1266:1294	functional permeability tests	1266:1294	Epithelial repair events were assessed histo-morphologically in combination with functional permeability tests, expression of key inflammatory and repairing factors, and evaluation of colon mucosa-adherent microbiota composition by 16S rRNA sequencing.
31367158	9	78	theme	repairing	1332:1340	arg1	factors					1342:1348	key inflammatory and repairing factors	1311:1348	key inflammatory and repairing factors	1311:1348	Epithelial repair events were assessed histo-morphologically in combination with functional permeability tests, expression of key inflammatory and repairing factors, and evaluation of colon mucosa-adherent microbiota composition by 16S rRNA sequencing.
31367158	4	79	theme	content	582:588	arg1	characteristics					590:604	the luminal content characteristics	570:604	the luminal content characteristics during this process	570:624	However, the sequence of events associated with colon mucosal repair after colitis and the evolution of the luminal content characteristics during this process have been little studied.
31367158	7	80	theme	DSS	969:971	arg1	removal					973:979	DSS removal	969:979	DSS removal	969:979	They were euthanized 2 (day 7), 5 (day 10), 8 (day 13), and 23 (day 28) d after DSS removal.
31367158	1	81	theme	inflammatory	159:170	arg1	diseases					178:185	inflammatory bowel diseases	159:185	inflammatory bowel diseases	159:185	BACKGROUND Mucosal healing has become a therapeutic goal to achieve stable remission in patients with inflammatory bowel diseases.
31367158	6	82	from	DSS	857:859	arg1	water					874:878	drinking water	865:878	drinking water	865:878	METHODS C57BL/6 male mice were given 3.5% dextran sodium sulfate (DSS) in drinking water for 5 d.
31367158	10	83	theme	function	1542:1549	arg1	alterations					1508:1518	maximal alterations	1500:1518	maximal alterations of intestinal barrier function and histological damage associated with goblet cell depletion in colon mucosa	1500:1627	RESULTS The maximal intensity of colitis was concomitant with maximal alterations of intestinal barrier function and histological damage associated with goblet cell depletion in colon mucosa.
31367158	6	84	theme	male	807:810	arg1	mice					812:815	METHODS C57BL/6 male mice	791:815	METHODS C57BL/6 male mice	791:815	METHODS C57BL/6 male mice were given 3.5% dextran sodium sulfate (DSS) in drinking water for 5 d.
31367158	12	85	theme	colitis	2412:2418	arg1	induction					2420:2428	colitis induction	2412:2428	colitis induction	2412:2428	Although signs of colitis were severe (inflammatory cell infiltrate, crypt disarray, increased permeability) and associated with colonic luminal alterations (hyperosmolarity, dysbiosis, decrease in short-chain fatty acid content), epithelial healing processes were launched early during the inflammatory flare with increased gene expression of certain key epithelial repair modulators, including transforming growth factor-β, interleukin (Il)-15, Il-22, Il-33, and serum amyloid A. Whereas signs of inflammation progressively diminished, luminal colonic environment alterations and microscopic abnormalities of colon mucosa persisted long after colitis induction.
31367158	8	86	theme	non-DSS	1133:1139	arg1	group					1157:1161	a non-DSS treated control group	1131:1161	a non-DSS treated control group	1131:1161	The colonic luminal environment and epithelial repair processes during the inflammatory flare and colitis resolution were analyzed with reference to a non-DSS treated control group, euthanized at day 0.
31367158	10	87	theme	intestinal	1523:1532	arg1	function					1542:1549	intestinal barrier function	1523:1549	intestinal barrier function	1523:1549	RESULTS The maximal intensity of colitis was concomitant with maximal alterations of intestinal barrier function and histological damage associated with goblet cell depletion in colon mucosa.
31367158	5	88	theme	characteristics	699:713	arg1	evolution					672:680	the evolution	668:680	the evolution of colon mucosal characteristics	668:713	AIM To document the evolution of colon mucosal characteristics during mucosal healing using a mouse model with chemically-induced colitis.
31367158	6	89	theme	METHODS	791:797	arg1	mice					812:815	METHODS C57BL/6 male mice	791:815	METHODS C57BL/6 male mice	791:815	METHODS C57BL/6 male mice were given 3.5% dextran sodium sulfate (DSS) in drinking water for 5 d.
31367158	13	90	theme	key	2630:2632	arg1	modulators					2634:2643	key modulators	2630:2643	key modulators	2630:2643	CONCLUSION This study shows that colon repair can be initiated in the context of inflamed mucosa associated with alterations of the luminal environment and highlights the longitudinal involvement of key modulators.
31367158	9	91	theme	colon	1369:1373	arg1	composition					1402:1412	colon mucosa-adherent microbiota composition	1369:1412	colon mucosa-adherent microbiota composition	1369:1412	Epithelial repair events were assessed histo-morphologically in combination with functional permeability tests, expression of key inflammatory and repairing factors, and evaluation of colon mucosa-adherent microbiota composition by 16S rRNA sequencing.
31367158	8	92	theme	colitis	1080:1086	arg1	resolution					1088:1097	colitis resolution	1080:1097	colitis resolution	1080:1097	The colonic luminal environment and epithelial repair processes during the inflammatory flare and colitis resolution were analyzed with reference to a non-DSS treated control group, euthanized at day 0.
31367158	8	93	theme	control	1149:1155	arg1	group					1157:1161	a non-DSS treated control group	1131:1161	a non-DSS treated control group	1131:1161	The colonic luminal environment and epithelial repair processes during the inflammatory flare and colitis resolution were analyzed with reference to a non-DSS treated control group, euthanized at day 0.
31367158	7	94	dep	d	961:961	arg1	removal					973:979	DSS removal	969:979	DSS removal	969:979	They were euthanized 2 (day 7), 5 (day 10), 8 (day 13), and 23 (day 28) d after DSS removal.
31367158	5	95	theme	mucosal	722:728	arg1	healing					730:736	mucosal healing	722:736	mucosal healing using a mouse model with chemically-induced colitis	722:788	AIM To document the evolution of colon mucosal characteristics during mucosal healing using a mouse model with chemically-induced colitis.
31367158	9	96	theme	microbiota	1391:1400	arg1	composition					1402:1412	colon mucosa-adherent microbiota composition	1369:1412	colon mucosa-adherent microbiota composition	1369:1412	Epithelial repair events were assessed histo-morphologically in combination with functional permeability tests, expression of key inflammatory and repairing factors, and evaluation of colon mucosa-adherent microbiota composition by 16S rRNA sequencing.
31367158	5	97	with	model	752:756	arg1	colitis					782:788	chemically-induced colitis	763:788	chemically-induced colitis	763:788	AIM To document the evolution of colon mucosal characteristics during mucosal healing using a mouse model with chemically-induced colitis.
31367158	10	98	theme	histological	1555:1566	arg1	damage					1568:1573	histological damage	1555:1573	histological damage	1555:1573	RESULTS The maximal intensity of colitis was concomitant with maximal alterations of intestinal barrier function and histological damage associated with goblet cell depletion in colon mucosa.
31367158	2	99	theme	overlapping	215:225	arg1	actions					227:233	overlapping actions	215:233	overlapping actions of complex cellular processes, such as migration, proliferation, and differentiation,	215:319	To achieve this objective, overlapping actions of complex cellular processes, such as migration, proliferation, and differentiation, are required.
31367158	5	100	theme	colon	685:689	arg1	characteristics					699:713	colon mucosal characteristics	685:713	colon mucosal characteristics	685:713	AIM To document the evolution of colon mucosal characteristics during mucosal healing using a mouse model with chemically-induced colitis.
31367158	9	101	theme	rRNA	1421:1424	arg1	sequencing					1426:1435	16S rRNA sequencing	1417:1435	16S rRNA sequencing	1417:1435	Epithelial repair events were assessed histo-morphologically in combination with functional permeability tests, expression of key inflammatory and repairing factors, and evaluation of colon mucosa-adherent microbiota composition by 16S rRNA sequencing.
31367158	12	102	theme	healing	2009:2015	arg1	processes					2017:2025	epithelial healing processes	1998:2025	epithelial healing processes	1998:2025	Although signs of colitis were severe (inflammatory cell infiltrate, crypt disarray, increased permeability) and associated with colonic luminal alterations (hyperosmolarity, dysbiosis, decrease in short-chain fatty acid content), epithelial healing processes were launched early during the inflammatory flare with increased gene expression of certain key epithelial repair modulators, including transforming growth factor-β, interleukin (Il)-15, Il-22, Il-33, and serum amyloid A. Whereas signs of inflammation progressively diminished, luminal colonic environment alterations and microscopic abnormalities of colon mucosa persisted long after colitis induction.
31367158	10	103	dep	RESULTS	1438:1444	arg1	intensity					1458:1466	The maximal intensity	1446:1466	The maximal intensity of colitis	1446:1477	RESULTS The maximal intensity of colitis was concomitant with maximal alterations of intestinal barrier function and histological damage associated with goblet cell depletion in colon mucosa.
31367158	10	103	dep	RESULTS	1438:1444	arg1	concomitant					1483:1493	concomitant	1483:1493	concomitant	1483:1493	RESULTS The maximal intensity of colitis was concomitant with maximal alterations of intestinal barrier function and histological damage associated with goblet cell depletion in colon mucosa.
31367158	2	104	theme	cellular	246:253	arg1	migration					274:282	migration	274:282	migration	274:282	To achieve this objective, overlapping actions of complex cellular processes, such as migration, proliferation, and differentiation, are required.
31367158	2	104	theme	cellular	246:253	arg1	processes					255:263	complex cellular processes	238:263	complex cellular processes	238:263	To achieve this objective, overlapping actions of complex cellular processes, such as migration, proliferation, and differentiation, are required.
31367158	2	104	theme	cellular	246:253	arg1	proliferation					285:297	proliferation	285:297	proliferation	285:297	To achieve this objective, overlapping actions of complex cellular processes, such as migration, proliferation, and differentiation, are required.
31367158	2	104	theme	cellular	246:253	arg1	differentiation					304:318	differentiation	304:318	differentiation	304:318	To achieve this objective, overlapping actions of complex cellular processes, such as migration, proliferation, and differentiation, are required.
31367158	12	105	dep	interleukin	2193:2203	arg1	A.					2246:2247	A.	2246:2247	A.	2246:2247	Although signs of colitis were severe (inflammatory cell infiltrate, crypt disarray, increased permeability) and associated with colonic luminal alterations (hyperosmolarity, dysbiosis, decrease in short-chain fatty acid content), epithelial healing processes were launched early during the inflammatory flare with increased gene expression of certain key epithelial repair modulators, including transforming growth factor-β, interleukin (Il)-15, Il-22, Il-33, and serum amyloid A. Whereas signs of inflammation progressively diminished, luminal colonic environment alterations and microscopic abnormalities of colon mucosa persisted long after colitis induction.
31367158	13	106	theme	environment	2571:2581	arg1	alterations					2544:2554	alterations	2544:2554	alterations of the luminal environment	2544:2581	CONCLUSION This study shows that colon repair can be initiated in the context of inflamed mucosa associated with alterations of the luminal environment and highlights the longitudinal involvement of key modulators.
31367158	12	107	theme	luminal	1904:1910	arg1	alterations					1912:1922	colonic luminal alterations	1896:1922	colonic luminal alterations (hyperosmolarity, dysbiosis, decrease in short-chain fatty acid content)	1896:1995	Although signs of colitis were severe (inflammatory cell infiltrate, crypt disarray, increased permeability) and associated with colonic luminal alterations (hyperosmolarity, dysbiosis, decrease in short-chain fatty acid content), epithelial healing processes were launched early during the inflammatory flare with increased gene expression of certain key epithelial repair modulators, including transforming growth factor-β, interleukin (Il)-15, Il-22, Il-33, and serum amyloid A. Whereas signs of inflammation progressively diminished, luminal colonic environment alterations and microscopic abnormalities of colon mucosa persisted long after colitis induction.
31367158	12	108	theme	fatty	1977:1981	arg1	content					1988:1994	short-chain fatty acid content	1965:1994	short-chain fatty acid content	1965:1994	Although signs of colitis were severe (inflammatory cell infiltrate, crypt disarray, increased permeability) and associated with colonic luminal alterations (hyperosmolarity, dysbiosis, decrease in short-chain fatty acid content), epithelial healing processes were launched early during the inflammatory flare with increased gene expression of certain key epithelial repair modulators, including transforming growth factor-β, interleukin (Il)-15, Il-22, Il-33, and serum amyloid A. Whereas signs of inflammation progressively diminished, luminal colonic environment alterations and microscopic abnormalities of colon mucosa persisted long after colitis induction.
31367158	11	109	theme	DSS-treatment	1675:1687	arg1	termination					1656:1666	termination	1656:1666	termination of the DSS-treatment	1656:1687	It was recorded 2 d after termination of the DSS-treatment, followed by a progressive return to values similar to those of control mice.
31367158	13	110	theme	longitudinal	2602:2613	arg1	involvement					2615:2625	the longitudinal involvement	2598:2625	the longitudinal involvement of key modulators	2598:2643	CONCLUSION This study shows that colon repair can be initiated in the context of inflamed mucosa associated with alterations of the luminal environment and highlights the longitudinal involvement of key modulators.
31367158	1	111	with	patients	145:152	arg1	diseases					178:185	inflammatory bowel diseases	159:185	inflammatory bowel diseases	159:185	BACKGROUND Mucosal healing has become a therapeutic goal to achieve stable remission in patients with inflammatory bowel diseases.
31367158	4	112	theme	colon	514:518	arg1	repair					528:533	colon mucosal repair	514:533	colon mucosal repair after colitis	514:547	However, the sequence of events associated with colon mucosal repair after colitis and the evolution of the luminal content characteristics during this process have been little studied.
31367158	12	113	theme	increased	1852:1860	arg1	infiltrate					1824:1833	inflammatory cell infiltrate	1806:1833	inflammatory cell infiltrate	1806:1833	Although signs of colitis were severe (inflammatory cell infiltrate, crypt disarray, increased permeability) and associated with colonic luminal alterations (hyperosmolarity, dysbiosis, decrease in short-chain fatty acid content), epithelial healing processes were launched early during the inflammatory flare with increased gene expression of certain key epithelial repair modulators, including transforming growth factor-β, interleukin (Il)-15, Il-22, Il-33, and serum amyloid A. Whereas signs of inflammation progressively diminished, luminal colonic environment alterations and microscopic abnormalities of colon mucosa persisted long after colitis induction.
31367158	12	113	theme	increased	1852:1860	arg1	permeability					1862:1873	increased permeability	1852:1873	increased permeability	1852:1873	Although signs of colitis were severe (inflammatory cell infiltrate, crypt disarray, increased permeability) and associated with colonic luminal alterations (hyperosmolarity, dysbiosis, decrease in short-chain fatty acid content), epithelial healing processes were launched early during the inflammatory flare with increased gene expression of certain key epithelial repair modulators, including transforming growth factor-β, interleukin (Il)-15, Il-22, Il-33, and serum amyloid A. Whereas signs of inflammation progressively diminished, luminal colonic environment alterations and microscopic abnormalities of colon mucosa persisted long after colitis induction.
31367158	12	114	theme	growth	2176:2181	arg1	factor-β					2183:2190	transforming growth factor-β	2163:2190	transforming growth factor-β	2163:2190	Although signs of colitis were severe (inflammatory cell infiltrate, crypt disarray, increased permeability) and associated with colonic luminal alterations (hyperosmolarity, dysbiosis, decrease in short-chain fatty acid content), epithelial healing processes were launched early during the inflammatory flare with increased gene expression of certain key epithelial repair modulators, including transforming growth factor-β, interleukin (Il)-15, Il-22, Il-33, and serum amyloid A. Whereas signs of inflammation progressively diminished, luminal colonic environment alterations and microscopic abnormalities of colon mucosa persisted long after colitis induction.
31367158	5	115	theme	mouse	746:750	arg1	model					752:756	a mouse model	744:756	a mouse model with chemically-induced colitis	744:788	AIM To document the evolution of colon mucosal characteristics during mucosal healing using a mouse model with chemically-induced colitis.
31367158	9	116	theme	repair	1196:1201	arg1	events					1203:1208	Epithelial repair events	1185:1208	Epithelial repair events	1185:1208	Epithelial repair events were assessed histo-morphologically in combination with functional permeability tests, expression of key inflammatory and repairing factors, and evaluation of colon mucosa-adherent microbiota composition by 16S rRNA sequencing.
31367158	12	117	theme	cell	1819:1822	arg1	infiltrate					1824:1833	inflammatory cell infiltrate	1806:1833	inflammatory cell infiltrate	1806:1833	Although signs of colitis were severe (inflammatory cell infiltrate, crypt disarray, increased permeability) and associated with colonic luminal alterations (hyperosmolarity, dysbiosis, decrease in short-chain fatty acid content), epithelial healing processes were launched early during the inflammatory flare with increased gene expression of certain key epithelial repair modulators, including transforming growth factor-β, interleukin (Il)-15, Il-22, Il-33, and serum amyloid A. Whereas signs of inflammation progressively diminished, luminal colonic environment alterations and microscopic abnormalities of colon mucosa persisted long after colitis induction.
31367158	12	117	theme	cell	1819:1822	arg1	disarray					1842:1849	crypt disarray	1836:1849	crypt disarray	1836:1849	Although signs of colitis were severe (inflammatory cell infiltrate, crypt disarray, increased permeability) and associated with colonic luminal alterations (hyperosmolarity, dysbiosis, decrease in short-chain fatty acid content), epithelial healing processes were launched early during the inflammatory flare with increased gene expression of certain key epithelial repair modulators, including transforming growth factor-β, interleukin (Il)-15, Il-22, Il-33, and serum amyloid A. Whereas signs of inflammation progressively diminished, luminal colonic environment alterations and microscopic abnormalities of colon mucosa persisted long after colitis induction.
31367158	12	117	theme	cell	1819:1822	arg1	permeability					1862:1873	increased permeability	1852:1873	increased permeability	1852:1873	Although signs of colitis were severe (inflammatory cell infiltrate, crypt disarray, increased permeability) and associated with colonic luminal alterations (hyperosmolarity, dysbiosis, decrease in short-chain fatty acid content), epithelial healing processes were launched early during the inflammatory flare with increased gene expression of certain key epithelial repair modulators, including transforming growth factor-β, interleukin (Il)-15, Il-22, Il-33, and serum amyloid A. Whereas signs of inflammation progressively diminished, luminal colonic environment alterations and microscopic abnormalities of colon mucosa persisted long after colitis induction.
31367158	1	118	theme	Mucosal	68:74	arg1	healing					76:82	BACKGROUND Mucosal healing	57:82	BACKGROUND Mucosal healing	57:82	BACKGROUND Mucosal healing has become a therapeutic goal to achieve stable remission in patients with inflammatory bowel diseases.
31367158	0	119	from	colitis	40:46	arg1	mice					51:54	mice	51:54	mice	51:54	Mucosal healing progression after acute colitis in mice.
31367158	10	120	theme	cell	1598:1601	arg1	depletion					1603:1611	goblet cell depletion	1591:1611	goblet cell depletion in colon mucosa	1591:1627	RESULTS The maximal intensity of colitis was concomitant with maximal alterations of intestinal barrier function and histological damage associated with goblet cell depletion in colon mucosa.
31367158	0	121	theme	healing	8:14	arg1	progression					16:26	Mucosal healing progression	0:26	Mucosal healing progression	0:26	Mucosal healing progression after acute colitis in mice.
31367158	12	122	theme	mucosa	2384:2389	arg1	abnormalities					2361:2373	microscopic abnormalities	2349:2373	microscopic abnormalities of colon mucosa	2349:2389	Although signs of colitis were severe (inflammatory cell infiltrate, crypt disarray, increased permeability) and associated with colonic luminal alterations (hyperosmolarity, dysbiosis, decrease in short-chain fatty acid content), epithelial healing processes were launched early during the inflammatory flare with increased gene expression of certain key epithelial repair modulators, including transforming growth factor-β, interleukin (Il)-15, Il-22, Il-33, and serum amyloid A. Whereas signs of inflammation progressively diminished, luminal colonic environment alterations and microscopic abnormalities of colon mucosa persisted long after colitis induction.
31367158	12	122	theme	mucosa	2384:2389	arg1	alterations					2333:2343	luminal colonic environment alterations	2305:2343	luminal colonic environment alterations	2305:2343	Although signs of colitis were severe (inflammatory cell infiltrate, crypt disarray, increased permeability) and associated with colonic luminal alterations (hyperosmolarity, dysbiosis, decrease in short-chain fatty acid content), epithelial healing processes were launched early during the inflammatory flare with increased gene expression of certain key epithelial repair modulators, including transforming growth factor-β, interleukin (Il)-15, Il-22, Il-33, and serum amyloid A. Whereas signs of inflammation progressively diminished, luminal colonic environment alterations and microscopic abnormalities of colon mucosa persisted long after colitis induction.
31367158	8	123	dep	environment	1002:1012	arg1	The					982:984	The	982:984	The	982:984	The colonic luminal environment and epithelial repair processes during the inflammatory flare and colitis resolution were analyzed with reference to a non-DSS treated control group, euthanized at day 0.
31367158	12	124	theme	modulators	2141:2150	arg1	expression					2097:2106	increased gene expression	2082:2106	increased gene expression of certain key epithelial repair modulators	2082:2150	Although signs of colitis were severe (inflammatory cell infiltrate, crypt disarray, increased permeability) and associated with colonic luminal alterations (hyperosmolarity, dysbiosis, decrease in short-chain fatty acid content), epithelial healing processes were launched early during the inflammatory flare with increased gene expression of certain key epithelial repair modulators, including transforming growth factor-β, interleukin (Il)-15, Il-22, Il-33, and serum amyloid A. Whereas signs of inflammation progressively diminished, luminal colonic environment alterations and microscopic abnormalities of colon mucosa persisted long after colitis induction.
31367158	12	125	theme	microscopic	2349:2359	arg1	abnormalities					2361:2373	microscopic abnormalities	2349:2373	microscopic abnormalities of colon mucosa	2349:2389	Although signs of colitis were severe (inflammatory cell infiltrate, crypt disarray, increased permeability) and associated with colonic luminal alterations (hyperosmolarity, dysbiosis, decrease in short-chain fatty acid content), epithelial healing processes were launched early during the inflammatory flare with increased gene expression of certain key epithelial repair modulators, including transforming growth factor-β, interleukin (Il)-15, Il-22, Il-33, and serum amyloid A. Whereas signs of inflammation progressively diminished, luminal colonic environment alterations and microscopic abnormalities of colon mucosa persisted long after colitis induction.
31367158	12	126	theme	certain	2111:2117	arg1	modulators					2141:2150	certain key epithelial repair modulators	2111:2150	certain key epithelial repair modulators	2111:2150	Although signs of colitis were severe (inflammatory cell infiltrate, crypt disarray, increased permeability) and associated with colonic luminal alterations (hyperosmolarity, dysbiosis, decrease in short-chain fatty acid content), epithelial healing processes were launched early during the inflammatory flare with increased gene expression of certain key epithelial repair modulators, including transforming growth factor-β, interleukin (Il)-15, Il-22, Il-33, and serum amyloid A. Whereas signs of inflammation progressively diminished, luminal colonic environment alterations and microscopic abnormalities of colon mucosa persisted long after colitis induction.
31367158	3	127	theme	proteins	456:463	arg1	range					427:431	a wide range	420:431	a wide range of distinct regulatory proteins	420:463	These events are longitudinally and tightly controlled by numerous factors including a wide range of distinct regulatory proteins.
31367158	13	128	theme	inflamed	2512:2519	arg1	mucosa					2521:2526	inflamed mucosa	2512:2526	inflamed mucosa associated with alterations of the luminal environment	2512:2581	CONCLUSION This study shows that colon repair can be initiated in the context of inflamed mucosa associated with alterations of the luminal environment and highlights the longitudinal involvement of key modulators.
31367158	9	129	theme	functional	1266:1275	arg1	tests					1290:1294	functional permeability tests	1266:1294	functional permeability tests	1266:1294	Epithelial repair events were assessed histo-morphologically in combination with functional permeability tests, expression of key inflammatory and repairing factors, and evaluation of colon mucosa-adherent microbiota composition by 16S rRNA sequencing.
31367158	12	130	theme	epithelial	2123:2132	arg1	modulators					2141:2150	certain key epithelial repair modulators	2111:2150	certain key epithelial repair modulators	2111:2150	Although signs of colitis were severe (inflammatory cell infiltrate, crypt disarray, increased permeability) and associated with colonic luminal alterations (hyperosmolarity, dysbiosis, decrease in short-chain fatty acid content), epithelial healing processes were launched early during the inflammatory flare with increased gene expression of certain key epithelial repair modulators, including transforming growth factor-β, interleukin (Il)-15, Il-22, Il-33, and serum amyloid A. Whereas signs of inflammation progressively diminished, luminal colonic environment alterations and microscopic abnormalities of colon mucosa persisted long after colitis induction.
31367158	8	131	theme	luminal	994:1000	arg1	environment					1002:1012	colonic luminal environment	986:1012	colonic luminal environment	986:1012	The colonic luminal environment and epithelial repair processes during the inflammatory flare and colitis resolution were analyzed with reference to a non-DSS treated control group, euthanized at day 0.
31367158	6	132	theme	sulfate	848:854	arg1	DSS					857:859	3.5% dextran sodium sulfate (DSS)	828:860	3.5% dextran sodium sulfate (DSS) in drinking water for 5 d	828:886	METHODS C57BL/6 male mice were given 3.5% dextran sodium sulfate (DSS) in drinking water for 5 d.
31367158	12	133	theme	colonic	2313:2319	arg1	environment					2321:2331	luminal colonic environment	2305:2331	luminal colonic environment alterations	2305:2343	Although signs of colitis were severe (inflammatory cell infiltrate, crypt disarray, increased permeability) and associated with colonic luminal alterations (hyperosmolarity, dysbiosis, decrease in short-chain fatty acid content), epithelial healing processes were launched early during the inflammatory flare with increased gene expression of certain key epithelial repair modulators, including transforming growth factor-β, interleukin (Il)-15, Il-22, Il-33, and serum amyloid A. Whereas signs of inflammation progressively diminished, luminal colonic environment alterations and microscopic abnormalities of colon mucosa persisted long after colitis induction.
31367158	12	134	theme	increased	2082:2090	arg1	expression					2097:2106	increased gene expression	2082:2106	increased gene expression of certain key epithelial repair modulators	2082:2150	Although signs of colitis were severe (inflammatory cell infiltrate, crypt disarray, increased permeability) and associated with colonic luminal alterations (hyperosmolarity, dysbiosis, decrease in short-chain fatty acid content), epithelial healing processes were launched early during the inflammatory flare with increased gene expression of certain key epithelial repair modulators, including transforming growth factor-β, interleukin (Il)-15, Il-22, Il-33, and serum amyloid A. Whereas signs of inflammation progressively diminished, luminal colonic environment alterations and microscopic abnormalities of colon mucosa persisted long after colitis induction.
31367158	6	135	theme	dextran	833:839	arg1	DSS					857:859	3.5% dextran sodium sulfate (DSS)	828:860	3.5% dextran sodium sulfate (DSS) in drinking water for 5 d	828:886	METHODS C57BL/6 male mice were given 3.5% dextran sodium sulfate (DSS) in drinking water for 5 d.
31367158	3	136	theme	distinct	436:443	arg1	proteins					456:463	distinct regulatory proteins	436:463	distinct regulatory proteins	436:463	These events are longitudinally and tightly controlled by numerous factors including a wide range of distinct regulatory proteins.
31367158	10	137	theme	colitis	1471:1477	arg1	intensity					1458:1466	The maximal intensity	1446:1466	The maximal intensity of colitis	1446:1477	RESULTS The maximal intensity of colitis was concomitant with maximal alterations of intestinal barrier function and histological damage associated with goblet cell depletion in colon mucosa.
31367158	10	137	theme	colitis	1471:1477	arg1	concomitant					1483:1493	concomitant	1483:1493	concomitant	1483:1493	RESULTS The maximal intensity of colitis was concomitant with maximal alterations of intestinal barrier function and histological damage associated with goblet cell depletion in colon mucosa.
31367158	9	138	theme	key	1311:1313	arg1	factors					1342:1348	key inflammatory and repairing factors	1311:1348	key inflammatory and repairing factors	1311:1348	Epithelial repair events were assessed histo-morphologically in combination with functional permeability tests, expression of key inflammatory and repairing factors, and evaluation of colon mucosa-adherent microbiota composition by 16S rRNA sequencing.
29562674	10	0	theme	HS	2219:2220	arg1	structure					2227:2235	HS fine structure	2219:2235	HS fine structure	2219:2235	The presence of significant quantities of disaccharides containing the monosaccharide GlcNAc6S contrasts with previous findings in Drosophila melanogaster and suggests subtle differences in HS fine structure between species of the Diptera.
29562674	8	1	theme	species	1577:1583	arg1	type					1556:1559	the type	1552:1559	the type of disaccharide species present between genders or between ages (24 vs. 48 h post emergence)	1552:1652	There were no significant differences in the type of disaccharide species present between genders or between ages (24 vs. 48 h post emergence), although the HS from female flies was more heavily sulphated overall.
29562674	3	2	theme	parasite	458:465	arg1	cycle					472:476	the parasite life cycle	454:476	the parasite life cycle	454:476	Precise details of the life cycle have yet to be established, but the parasite life cycle involves crossing the insect peritrophic matrix (PM).
29562674	6	3	theme	PMs	861:863	arg1	removal					850:856	removal	850:856	removal of PMs from the insects	850:880	Following removal of PMs from the insects and the application of heparinases (bacterial lyase enzymes that are specific for heparan sulphate (HS) GAG polysaccharides), dot blots with a HS-specific antibody showed heparan sulphate proteoglycans (HSPGs) to be present, consistent with Glossina morsitans morsitans genome analysis, as well as the likely expression of the HSPGs syndecan and perlecan.
29562674	6	3	theme	PMs	861:863	arg1	application					890:900	the application	886:900	the application of heparinases (bacterial lyase enzymes that are specific for heparan sulphate (HS) GAG polysaccharides)	886:1005	Following removal of PMs from the insects and the application of heparinases (bacterial lyase enzymes that are specific for heparan sulphate (HS) GAG polysaccharides), dot blots with a HS-specific antibody showed heparan sulphate proteoglycans (HSPGs) to be present, consistent with Glossina morsitans morsitans genome analysis, as well as the likely expression of the HSPGs syndecan and perlecan.
29562674	9	4	theme	2-O-sulphation	1864:1877	arg1	levels					1854:1859	overall levels	1846:1859	overall levels of 2-O-sulphation	1846:1877	Significant differences, which may relate to differences in infection between genders or ages, were evident, however, in overall levels of 2-O-sulphation between sexes and, for females, between 24 and 48 h post-emergence, implying a change in expression or activity for the 2-O-sulphotransferase enzyme.
29562674	3	5	theme	insect	500:505	arg1	matrix					519:524	the insect peritrophic matrix	496:524	the insect peritrophic matrix (PM)	496:529	Precise details of the life cycle have yet to be established, but the parasite life cycle involves crossing the insect peritrophic matrix (PM).
29562674	3	5	theme	insect	500:505	arg1	PM					527:528	PM	527:528	PM	527:528	Precise details of the life cycle have yet to be established, but the parasite life cycle involves crossing the insect peritrophic matrix (PM).
29562674	6	6	dep	HSPGs	1209:1213	arg1	perlecan					1228:1235	perlecan	1228:1235	perlecan	1228:1235	Following removal of PMs from the insects and the application of heparinases (bacterial lyase enzymes that are specific for heparan sulphate (HS) GAG polysaccharides), dot blots with a HS-specific antibody showed heparan sulphate proteoglycans (HSPGs) to be present, consistent with Glossina morsitans morsitans genome analysis, as well as the likely expression of the HSPGs syndecan and perlecan.
29562674	6	6	dep	HSPGs	1209:1213	arg1	syndecan					1215:1222	syndecan	1215:1222	syndecan	1215:1222	Following removal of PMs from the insects and the application of heparinases (bacterial lyase enzymes that are specific for heparan sulphate (HS) GAG polysaccharides), dot blots with a HS-specific antibody showed heparan sulphate proteoglycans (HSPGs) to be present, consistent with Glossina morsitans morsitans genome analysis, as well as the likely expression of the HSPGs syndecan and perlecan.
29562674	6	6	dep	HSPGs	1209:1213	arg1	HSPGs					1209:1213	the HSPGs syndecan and perlecan	1205:1235	the HSPGs syndecan and perlecan	1205:1235	Following removal of PMs from the insects and the application of heparinases (bacterial lyase enzymes that are specific for heparan sulphate (HS) GAG polysaccharides), dot blots with a HS-specific antibody showed heparan sulphate proteoglycans (HSPGs) to be present, consistent with Glossina morsitans morsitans genome analysis, as well as the likely expression of the HSPGs syndecan and perlecan.
29562674	2	7	theme	fly	258:260	arg1	fly					258:260	the tsetse fly species	247:268	the tsetse fly species	247:268	One of the tsetse fly species, Glossina morsitans morsitans, is host to the parasite, Trypanosoma brucei, a major cause of African trypanosomiasis.
29562674	2	7	theme	fly	258:260	arg1	Glossina					271:278	Glossina	271:278	Glossina	271:278	One of the tsetse fly species, Glossina morsitans morsitans, is host to the parasite, Trypanosoma brucei, a major cause of African trypanosomiasis.
29562674	2	7	theme	fly	258:260	arg1	One					240:242	One	240:242	One	240:242	One of the tsetse fly species, Glossina morsitans morsitans, is host to the parasite, Trypanosoma brucei, a major cause of African trypanosomiasis.
29562674	7	8	theme	exchange	1388:1395	arg1	chromatography					1397:1410	strong anion exchange chromatography	1375:1410	strong anion exchange chromatography	1375:1410	Exhaustive HS digestion with heparinases, fluorescent labeling of the resulting disaccharides with BODIPY fluorophore, and separation by strong anion exchange chromatography then demonstrated the presence of HS for the first time and provided the disaccharide composition.
29562674	1	9	theme	trypanosomes-sleeping	177:197	arg1	sickness					199:206	African trypanosomes-sleeping sickness	169:206	African trypanosomes-sleeping sickness	169:206	Tsetse flies are the principal insect vectors of African trypanosomes-sleeping sickness in humans and Nagana in cattle.
29562674	6	10	theme	bacterial	918:926	arg1	enzymes					934:940	bacterial lyase enzymes	918:940	bacterial lyase enzymes that are specific for heparan sulphate (HS) GAG polysaccharides	918:1004	Following removal of PMs from the insects and the application of heparinases (bacterial lyase enzymes that are specific for heparan sulphate (HS) GAG polysaccharides), dot blots with a HS-specific antibody showed heparan sulphate proteoglycans (HSPGs) to be present, consistent with Glossina morsitans morsitans genome analysis, as well as the likely expression of the HSPGs syndecan and perlecan.
29562674	9	11	from	differences	1770:1780	arg1	infection					1785:1793	infection	1785:1793	infection	1785:1793	Significant differences, which may relate to differences in infection between genders or ages, were evident, however, in overall levels of 2-O-sulphation between sexes and, for females, between 24 and 48 h post-emergence, implying a change in expression or activity for the 2-O-sulphotransferase enzyme.
29562674	6	12	theme	heparinases	905:915	arg1	removal					850:856	removal	850:856	removal of PMs from the insects	850:880	Following removal of PMs from the insects and the application of heparinases (bacterial lyase enzymes that are specific for heparan sulphate (HS) GAG polysaccharides), dot blots with a HS-specific antibody showed heparan sulphate proteoglycans (HSPGs) to be present, consistent with Glossina morsitans morsitans genome analysis, as well as the likely expression of the HSPGs syndecan and perlecan.
29562674	6	12	theme	heparinases	905:915	arg1	application					890:900	the application	886:900	the application of heparinases (bacterial lyase enzymes that are specific for heparan sulphate (HS) GAG polysaccharides)	886:1005	Following removal of PMs from the insects and the application of heparinases (bacterial lyase enzymes that are specific for heparan sulphate (HS) GAG polysaccharides), dot blots with a HS-specific antibody showed heparan sulphate proteoglycans (HSPGs) to be present, consistent with Glossina morsitans morsitans genome analysis, as well as the likely expression of the HSPGs syndecan and perlecan.
29562674	7	13	theme	resulting	1308:1316	arg1	disaccharides					1318:1330	the resulting disaccharides	1304:1330	the resulting disaccharides	1304:1330	Exhaustive HS digestion with heparinases, fluorescent labeling of the resulting disaccharides with BODIPY fluorophore, and separation by strong anion exchange chromatography then demonstrated the presence of HS for the first time and provided the disaccharide composition.
29562674	5	14	theme	conclusive	764:773	arg1	identification					775:788	their conclusive identification	758:788	their conclusive identification	758:788	Owing to the technical challenges of detecting small amounts of GAG polysaccharides, their conclusive identification and composition have not been possible until now.
29562674	1	15	from	humans	211:216	arg1	cattle					232:237	cattle	232:237	cattle	232:237	Tsetse flies are the principal insect vectors of African trypanosomes-sleeping sickness in humans and Nagana in cattle.
29562674	1	16	from	Nagana	222:227	arg1	cattle					232:237	cattle	232:237	cattle	232:237	Tsetse flies are the principal insect vectors of African trypanosomes-sleeping sickness in humans and Nagana in cattle.
29562674	6	17	dep	Glossina	1123:1130	arg1	morsitans					1142:1150	Glossina morsitans morsitans	1123:1150	Glossina morsitans morsitans genome analysis	1123:1166	Following removal of PMs from the insects and the application of heparinases (bacterial lyase enzymes that are specific for heparan sulphate (HS) GAG polysaccharides), dot blots with a HS-specific antibody showed heparan sulphate proteoglycans (HSPGs) to be present, consistent with Glossina morsitans morsitans genome analysis, as well as the likely expression of the HSPGs syndecan and perlecan.
29562674	6	18	dep	heparinases	905:915	arg1	enzymes					934:940	bacterial lyase enzymes	918:940	bacterial lyase enzymes that are specific for heparan sulphate (HS) GAG polysaccharides	918:1004	Following removal of PMs from the insects and the application of heparinases (bacterial lyase enzymes that are specific for heparan sulphate (HS) GAG polysaccharides), dot blots with a HS-specific antibody showed heparan sulphate proteoglycans (HSPGs) to be present, consistent with Glossina morsitans morsitans genome analysis, as well as the likely expression of the HSPGs syndecan and perlecan.
29562674	7	19	theme	fluorescent	1280:1290	arg1	labeling					1292:1299	fluorescent labeling	1280:1299	fluorescent labeling of the resulting disaccharides with BODIPY fluorophore	1280:1354	Exhaustive HS digestion with heparinases, fluorescent labeling of the resulting disaccharides with BODIPY fluorophore, and separation by strong anion exchange chromatography then demonstrated the presence of HS for the first time and provided the disaccharide composition.
29562674	10	20	theme	quantities	2057:2066	arg1	presence					2033:2040	The presence	2029:2040	The presence of significant quantities of disaccharides containing the monosaccharide GlcNAc6S	2029:2122	The presence of significant quantities of disaccharides containing the monosaccharide GlcNAc6S contrasts with previous findings in Drosophila melanogaster and suggests subtle differences in HS fine structure between species of the Diptera.
29562674	6	21	theme	sulphate	972:979	arg1	polysaccharides					990:1004	heparan sulphate (HS) GAG polysaccharides	964:1004	heparan sulphate (HS) GAG polysaccharides	964:1004	Following removal of PMs from the insects and the application of heparinases (bacterial lyase enzymes that are specific for heparan sulphate (HS) GAG polysaccharides), dot blots with a HS-specific antibody showed heparan sulphate proteoglycans (HSPGs) to be present, consistent with Glossina morsitans morsitans genome analysis, as well as the likely expression of the HSPGs syndecan and perlecan.
29562674	0	22	from	Fly	85:87	arg1	Composition					37:47	the Peritrophic Matrix Composition	14:47	the Peritrophic Matrix Composition of Heparan Sulphate from the Tsetse Fly	14:87	Variations in the Peritrophic Matrix Composition of Heparan Sulphate from the Tsetse Fly, Glossina morsitans morsitans.
29562674	0	22	from	Fly	85:87	arg1	Variations					0:9	Variations	0:9	Variations in the Peritrophic Matrix Composition of Heparan Sulphate from the Tsetse Fly	0:87	Variations in the Peritrophic Matrix Composition of Heparan Sulphate from the Tsetse Fly, Glossina morsitans morsitans.
29562674	6	23	theme	Glossina	1123:1130	arg1	analysis					1159:1166	Glossina morsitans morsitans genome analysis	1123:1166	Glossina morsitans morsitans genome analysis	1123:1166	Following removal of PMs from the insects and the application of heparinases (bacterial lyase enzymes that are specific for heparan sulphate (HS) GAG polysaccharides), dot blots with a HS-specific antibody showed heparan sulphate proteoglycans (HSPGs) to be present, consistent with Glossina morsitans morsitans genome analysis, as well as the likely expression of the HSPGs syndecan and perlecan.
29562674	0	24	theme	Sulphate	60:67	arg1	Composition					37:47	the Peritrophic Matrix Composition	14:47	the Peritrophic Matrix Composition of Heparan Sulphate from the Tsetse Fly	14:87	Variations in the Peritrophic Matrix Composition of Heparan Sulphate from the Tsetse Fly, Glossina morsitans morsitans.
29562674	1	25	from	vectors	158:164	arg1	Nagana					222:227	Nagana	222:227	Nagana in cattle	222:237	Tsetse flies are the principal insect vectors of African trypanosomes-sleeping sickness in humans and Nagana in cattle.
29562674	1	25	from	vectors	158:164	arg1	humans					211:216	humans	211:216	humans	211:216	Tsetse flies are the principal insect vectors of African trypanosomes-sleeping sickness in humans and Nagana in cattle.
29562674	2	26	dep	Glossina	271:278	arg1	morsitans					290:298	Glossina morsitans morsitans	271:298	Glossina morsitans morsitans	271:298	One of the tsetse fly species, Glossina morsitans morsitans, is host to the parasite, Trypanosoma brucei, a major cause of African trypanosomiasis.
29562674	5	27	theme	small	720:724	arg1	amounts					726:732	small amounts	720:732	small amounts of GAG polysaccharides	720:755	Owing to the technical challenges of detecting small amounts of GAG polysaccharides, their conclusive identification and composition have not been possible until now.
29562674	5	27	theme	small	720:724	arg1	polysaccharides					741:755	GAG polysaccharides	737:755	GAG polysaccharides	737:755	Owing to the technical challenges of detecting small amounts of GAG polysaccharides, their conclusive identification and composition have not been possible until now.
29562674	1	28	theme	insect	151:156	arg1	vectors					158:164	the principal insect vectors	137:164	the principal insect vectors of African trypanosomes-sleeping sickness in humans and Nagana in cattle	137:237	Tsetse flies are the principal insect vectors of African trypanosomes-sleeping sickness in humans and Nagana in cattle.
29562674	1	28	theme	insect	151:156	arg1	flies					127:131	Tsetse flies	120:131	Tsetse flies	120:131	Tsetse flies are the principal insect vectors of African trypanosomes-sleeping sickness in humans and Nagana in cattle.
29562674	0	29	from	Variations	0:9	arg1	Composition					37:47	the Peritrophic Matrix Composition	14:47	the Peritrophic Matrix Composition of Heparan Sulphate from the Tsetse Fly	14:87	Variations in the Peritrophic Matrix Composition of Heparan Sulphate from the Tsetse Fly, Glossina morsitans morsitans.
29562674	0	29	from	Variations	0:9	arg1	Fly					85:87	the Tsetse Fly	74:87	the Tsetse Fly	74:87	Variations in the Peritrophic Matrix Composition of Heparan Sulphate from the Tsetse Fly, Glossina morsitans morsitans.
29562674	0	30	dep	Glossina	90:97	arg1	Variations					0:9	Variations	0:9	Variations in the Peritrophic Matrix Composition of Heparan Sulphate from the Tsetse Fly	0:87	Variations in the Peritrophic Matrix Composition of Heparan Sulphate from the Tsetse Fly, Glossina morsitans morsitans.
29562674	0	30	dep	Glossina	90:97	arg1	morsitans					109:117	Glossina morsitans morsitans	90:117	Glossina morsitans morsitans	90:117	Variations in the Peritrophic Matrix Composition of Heparan Sulphate from the Tsetse Fly, Glossina morsitans morsitans.
29562674	5	31	theme	technical	686:694	arg1	challenges					696:705	the technical challenges	682:705	the technical challenges of detecting small amounts of GAG polysaccharides	682:755	Owing to the technical challenges of detecting small amounts of GAG polysaccharides, their conclusive identification and composition have not been possible until now.
29562674	6	32	theme	dot	1008:1010	arg1	blots					1012:1016	dot blots	1008:1016	dot blots with a HS-specific antibody	1008:1044	Following removal of PMs from the insects and the application of heparinases (bacterial lyase enzymes that are specific for heparan sulphate (HS) GAG polysaccharides), dot blots with a HS-specific antibody showed heparan sulphate proteoglycans (HSPGs) to be present, consistent with Glossina morsitans morsitans genome analysis, as well as the likely expression of the HSPGs syndecan and perlecan.
29562674	9	33	theme	h	1929:1929	arg1	post-emergence					1931:1944	24 and 48 h post-emergence	1919:1944	24 and 48 h post-emergence	1919:1944	Significant differences, which may relate to differences in infection between genders or ages, were evident, however, in overall levels of 2-O-sulphation between sexes and, for females, between 24 and 48 h post-emergence, implying a change in expression or activity for the 2-O-sulphotransferase enzyme.
29562674	2	34	theme	major	348:352	arg1	cause					354:358	a major cause	346:358	a major cause of African trypanosomiasis	346:385	One of the tsetse fly species, Glossina morsitans morsitans, is host to the parasite, Trypanosoma brucei, a major cause of African trypanosomiasis.
29562674	2	34	theme	major	348:352	arg1	brucei					338:343	Trypanosoma brucei	326:343	Trypanosoma brucei	326:343	One of the tsetse fly species, Glossina morsitans morsitans, is host to the parasite, Trypanosoma brucei, a major cause of African trypanosomiasis.
29562674	8	35	from	differences	1537:1547	arg1	type					1556:1559	the type	1552:1559	the type of disaccharide species present between genders or between ages (24 vs. 48 h post emergence)	1552:1652	There were no significant differences in the type of disaccharide species present between genders or between ages (24 vs. 48 h post emergence), although the HS from female flies was more heavily sulphated overall.
29562674	7	36	theme	HS	1249:1250	arg1	digestion					1252:1260	Exhaustive HS digestion	1238:1260	Exhaustive HS digestion with heparinases	1238:1277	Exhaustive HS digestion with heparinases, fluorescent labeling of the resulting disaccharides with BODIPY fluorophore, and separation by strong anion exchange chromatography then demonstrated the presence of HS for the first time and provided the disaccharide composition.
29562674	6	37	theme	GAG	986:988	arg1	polysaccharides					990:1004	heparan sulphate (HS) GAG polysaccharides	964:1004	heparan sulphate (HS) GAG polysaccharides	964:1004	Following removal of PMs from the insects and the application of heparinases (bacterial lyase enzymes that are specific for heparan sulphate (HS) GAG polysaccharides), dot blots with a HS-specific antibody showed heparan sulphate proteoglycans (HSPGs) to be present, consistent with Glossina morsitans morsitans genome analysis, as well as the likely expression of the HSPGs syndecan and perlecan.
29562674	2	38	theme	trypanosomiasis	371:385	arg1	cause					354:358	a major cause	346:358	a major cause of African trypanosomiasis	346:385	One of the tsetse fly species, Glossina morsitans morsitans, is host to the parasite, Trypanosoma brucei, a major cause of African trypanosomiasis.
29562674	2	38	theme	trypanosomiasis	371:385	arg1	brucei					338:343	Trypanosoma brucei	326:343	Trypanosoma brucei	326:343	One of the tsetse fly species, Glossina morsitans morsitans, is host to the parasite, Trypanosoma brucei, a major cause of African trypanosomiasis.
29562674	3	39	theme	cycle	416:420	arg1	details					396:402	Precise details	388:402	Precise details of the life cycle	388:420	Precise details of the life cycle have yet to be established, but the parasite life cycle involves crossing the insect peritrophic matrix (PM).
29562674	7	40	with	separation	1361:1370	arg1	fluorophore					1344:1354	BODIPY fluorophore	1337:1354	BODIPY fluorophore	1337:1354	Exhaustive HS digestion with heparinases, fluorescent labeling of the resulting disaccharides with BODIPY fluorophore, and separation by strong anion exchange chromatography then demonstrated the presence of HS for the first time and provided the disaccharide composition.
29562674	7	40	with	separation	1361:1370	arg1	heparinases					1267:1277	heparinases	1267:1277	heparinases	1267:1277	Exhaustive HS digestion with heparinases, fluorescent labeling of the resulting disaccharides with BODIPY fluorophore, and separation by strong anion exchange chromatography then demonstrated the presence of HS for the first time and provided the disaccharide composition.
29562674	0	41	theme	Matrix	30:35	arg1	Composition					37:47	the Peritrophic Matrix Composition	14:47	the Peritrophic Matrix Composition of Heparan Sulphate from the Tsetse Fly	14:87	Variations in the Peritrophic Matrix Composition of Heparan Sulphate from the Tsetse Fly, Glossina morsitans morsitans.
29562674	6	42	with	blots	1012:1016	arg1	antibody					1037:1044	a HS-specific antibody	1023:1044	a HS-specific antibody	1023:1044	Following removal of PMs from the insects and the application of heparinases (bacterial lyase enzymes that are specific for heparan sulphate (HS) GAG polysaccharides), dot blots with a HS-specific antibody showed heparan sulphate proteoglycans (HSPGs) to be present, consistent with Glossina morsitans morsitans genome analysis, as well as the likely expression of the HSPGs syndecan and perlecan.
29562674	2	43	theme	parasite	316:323	arg1	cause					354:358	a major cause	346:358	a major cause of African trypanosomiasis	346:385	One of the tsetse fly species, Glossina morsitans morsitans, is host to the parasite, Trypanosoma brucei, a major cause of African trypanosomiasis.
29562674	2	43	theme	parasite	316:323	arg1	brucei					338:343	Trypanosoma brucei	326:343	Trypanosoma brucei	326:343	One of the tsetse fly species, Glossina morsitans morsitans, is host to the parasite, Trypanosoma brucei, a major cause of African trypanosomiasis.
29562674	5	44	theme	polysaccharides	741:755	arg1	amounts					726:732	small amounts	720:732	small amounts of GAG polysaccharides	720:755	Owing to the technical challenges of detecting small amounts of GAG polysaccharides, their conclusive identification and composition have not been possible until now.
29562674	5	44	theme	polysaccharides	741:755	arg1	polysaccharides					741:755	GAG polysaccharides	737:755	GAG polysaccharides	737:755	Owing to the technical challenges of detecting small amounts of GAG polysaccharides, their conclusive identification and composition have not been possible until now.
29562674	6	45	theme	sulphate	1061:1068	arg1	HSPGs					1085:1089	HSPGs	1085:1089	HSPGs	1085:1089	Following removal of PMs from the insects and the application of heparinases (bacterial lyase enzymes that are specific for heparan sulphate (HS) GAG polysaccharides), dot blots with a HS-specific antibody showed heparan sulphate proteoglycans (HSPGs) to be present, consistent with Glossina morsitans morsitans genome analysis, as well as the likely expression of the HSPGs syndecan and perlecan.
29562674	6	45	theme	sulphate	1061:1068	arg1	proteoglycans					1070:1082	heparan sulphate proteoglycans	1053:1082	heparan sulphate proteoglycans (HSPGs)	1053:1090	Following removal of PMs from the insects and the application of heparinases (bacterial lyase enzymes that are specific for heparan sulphate (HS) GAG polysaccharides), dot blots with a HS-specific antibody showed heparan sulphate proteoglycans (HSPGs) to be present, consistent with Glossina morsitans morsitans genome analysis, as well as the likely expression of the HSPGs syndecan and perlecan.
29562674	8	46	theme	significant	1525:1535	arg1	differences					1537:1547	no significant differences	1522:1547	no significant differences in the type of disaccharide species present between genders or between ages (24 vs. 48 h post emergence)	1522:1652	There were no significant differences in the type of disaccharide species present between genders or between ages (24 vs. 48 h post emergence), although the HS from female flies was more heavily sulphated overall.
29562674	7	47	theme	HS	1446:1447	arg1	presence					1434:1441	the presence	1430:1441	the presence of HS for the first time	1430:1466	Exhaustive HS digestion with heparinases, fluorescent labeling of the resulting disaccharides with BODIPY fluorophore, and separation by strong anion exchange chromatography then demonstrated the presence of HS for the first time and provided the disaccharide composition.
29562674	2	48	dep	fly	258:260	arg1	species					262:268	species	262:268	the tsetse fly species	247:268	One of the tsetse fly species, Glossina morsitans morsitans, is host to the parasite, Trypanosoma brucei, a major cause of African trypanosomiasis.
29562674	3	49	theme	life	467:470	arg1	cycle					472:476	the parasite life cycle	454:476	the parasite life cycle	454:476	Precise details of the life cycle have yet to be established, but the parasite life cycle involves crossing the insect peritrophic matrix (PM).
29562674	5	50	theme	GAG	737:739	arg1	polysaccharides					741:755	GAG polysaccharides	737:755	GAG polysaccharides	737:755	Owing to the technical challenges of detecting small amounts of GAG polysaccharides, their conclusive identification and composition have not been possible until now.
29562674	6	51	theme	HS-specific	1025:1035	arg1	antibody					1037:1044	a HS-specific antibody	1023:1044	a HS-specific antibody	1023:1044	Following removal of PMs from the insects and the application of heparinases (bacterial lyase enzymes that are specific for heparan sulphate (HS) GAG polysaccharides), dot blots with a HS-specific antibody showed heparan sulphate proteoglycans (HSPGs) to be present, consistent with Glossina morsitans morsitans genome analysis, as well as the likely expression of the HSPGs syndecan and perlecan.
29562674	6	52	from	insects	874:880	arg1	removal					850:856	removal	850:856	removal of PMs from the insects	850:880	Following removal of PMs from the insects and the application of heparinases (bacterial lyase enzymes that are specific for heparan sulphate (HS) GAG polysaccharides), dot blots with a HS-specific antibody showed heparan sulphate proteoglycans (HSPGs) to be present, consistent with Glossina morsitans morsitans genome analysis, as well as the likely expression of the HSPGs syndecan and perlecan.
29562674	6	52	from	insects	874:880	arg1	application					890:900	the application	886:900	the application of heparinases (bacterial lyase enzymes that are specific for heparan sulphate (HS) GAG polysaccharides)	886:1005	Following removal of PMs from the insects and the application of heparinases (bacterial lyase enzymes that are specific for heparan sulphate (HS) GAG polysaccharides), dot blots with a HS-specific antibody showed heparan sulphate proteoglycans (HSPGs) to be present, consistent with Glossina morsitans morsitans genome analysis, as well as the likely expression of the HSPGs syndecan and perlecan.
29562674	9	53	theme	2-O-sulphotransferase	1999:2019	arg1	enzyme					2021:2026	the 2-O-sulphotransferase enzyme	1995:2026	the 2-O-sulphotransferase enzyme	1995:2026	Significant differences, which may relate to differences in infection between genders or ages, were evident, however, in overall levels of 2-O-sulphation between sexes and, for females, between 24 and 48 h post-emergence, implying a change in expression or activity for the 2-O-sulphotransferase enzyme.
29562674	6	54	theme	HSPGs	1209:1213	arg1	analysis					1159:1166	Glossina morsitans morsitans genome analysis	1123:1166	Glossina morsitans morsitans genome analysis	1123:1166	Following removal of PMs from the insects and the application of heparinases (bacterial lyase enzymes that are specific for heparan sulphate (HS) GAG polysaccharides), dot blots with a HS-specific antibody showed heparan sulphate proteoglycans (HSPGs) to be present, consistent with Glossina morsitans morsitans genome analysis, as well as the likely expression of the HSPGs syndecan and perlecan.
29562674	6	54	theme	HSPGs	1209:1213	arg1	expression					1191:1200	the likely expression	1180:1200	the likely expression of the HSPGs syndecan and perlecan	1180:1235	Following removal of PMs from the insects and the application of heparinases (bacterial lyase enzymes that are specific for heparan sulphate (HS) GAG polysaccharides), dot blots with a HS-specific antibody showed heparan sulphate proteoglycans (HSPGs) to be present, consistent with Glossina morsitans morsitans genome analysis, as well as the likely expression of the HSPGs syndecan and perlecan.
29562674	2	55	theme	Trypanosoma	326:336	arg1	cause					354:358	a major cause	346:358	a major cause of African trypanosomiasis	346:385	One of the tsetse fly species, Glossina morsitans morsitans, is host to the parasite, Trypanosoma brucei, a major cause of African trypanosomiasis.
29562674	2	55	theme	Trypanosoma	326:336	arg1	brucei					338:343	Trypanosoma brucei	326:343	Trypanosoma brucei	326:343	One of the tsetse fly species, Glossina morsitans morsitans, is host to the parasite, Trypanosoma brucei, a major cause of African trypanosomiasis.
29562674	8	56	theme	disaccharide	1564:1575	arg1	species					1577:1583	disaccharide species	1564:1583	disaccharide species present between genders or between ages (24 vs. 48 h post emergence)	1564:1652	There were no significant differences in the type of disaccharide species present between genders or between ages (24 vs. 48 h post emergence), although the HS from female flies was more heavily sulphated overall.
29562674	10	57	theme	subtle	2197:2202	arg1	differences					2204:2214	subtle differences	2197:2214	subtle differences in HS fine structure between species of the Diptera	2197:2266	The presence of significant quantities of disaccharides containing the monosaccharide GlcNAc6S contrasts with previous findings in Drosophila melanogaster and suggests subtle differences in HS fine structure between species of the Diptera.
29562674	7	58	theme	strong	1375:1380	arg1	chromatography					1397:1410	strong anion exchange chromatography	1375:1410	strong anion exchange chromatography	1375:1410	Exhaustive HS digestion with heparinases, fluorescent labeling of the resulting disaccharides with BODIPY fluorophore, and separation by strong anion exchange chromatography then demonstrated the presence of HS for the first time and provided the disaccharide composition.
29562674	8	59	theme	present	1585:1591	arg1	species					1577:1583	disaccharide species	1564:1583	disaccharide species present between genders or between ages (24 vs. 48 h post emergence)	1564:1652	There were no significant differences in the type of disaccharide species present between genders or between ages (24 vs. 48 h post emergence), although the HS from female flies was more heavily sulphated overall.
29562674	6	60	theme	likely	1184:1189	arg1	expression					1191:1200	the likely expression	1180:1200	the likely expression of the HSPGs syndecan and perlecan	1180:1235	Following removal of PMs from the insects and the application of heparinases (bacterial lyase enzymes that are specific for heparan sulphate (HS) GAG polysaccharides), dot blots with a HS-specific antibody showed heparan sulphate proteoglycans (HSPGs) to be present, consistent with Glossina morsitans morsitans genome analysis, as well as the likely expression of the HSPGs syndecan and perlecan.
29562674	7	61	theme	anion	1382:1386	arg1	chromatography					1397:1410	strong anion exchange chromatography	1375:1410	strong anion exchange chromatography	1375:1410	Exhaustive HS digestion with heparinases, fluorescent labeling of the resulting disaccharides with BODIPY fluorophore, and separation by strong anion exchange chromatography then demonstrated the presence of HS for the first time and provided the disaccharide composition.
29562674	8	62	from	flies	1683:1687	arg1	sulphated					1706:1714	sulphated	1706:1714	sulphated	1706:1714	There were no significant differences in the type of disaccharide species present between genders or between ages (24 vs. 48 h post emergence), although the HS from female flies was more heavily sulphated overall.
29562674	8	62	from	flies	1683:1687	arg1	HS					1668:1669	the HS	1664:1669	the HS from female flies	1664:1687	There were no significant differences in the type of disaccharide species present between genders or between ages (24 vs. 48 h post emergence), although the HS from female flies was more heavily sulphated overall.
29562674	7	63	with	labeling	1292:1299	arg1	fluorophore					1344:1354	BODIPY fluorophore	1337:1354	BODIPY fluorophore	1337:1354	Exhaustive HS digestion with heparinases, fluorescent labeling of the resulting disaccharides with BODIPY fluorophore, and separation by strong anion exchange chromatography then demonstrated the presence of HS for the first time and provided the disaccharide composition.
29562674	7	63	with	labeling	1292:1299	arg1	heparinases					1267:1277	heparinases	1267:1277	heparinases	1267:1277	Exhaustive HS digestion with heparinases, fluorescent labeling of the resulting disaccharides with BODIPY fluorophore, and separation by strong anion exchange chromatography then demonstrated the presence of HS for the first time and provided the disaccharide composition.
29562674	1	64	theme	African	169:175	arg1	sickness					199:206	African trypanosomes-sleeping sickness	169:206	African trypanosomes-sleeping sickness	169:206	Tsetse flies are the principal insect vectors of African trypanosomes-sleeping sickness in humans and Nagana in cattle.
29562674	6	65	theme	lyase	928:932	arg1	enzymes					934:940	bacterial lyase enzymes	918:940	bacterial lyase enzymes that are specific for heparan sulphate (HS) GAG polysaccharides	918:1004	Following removal of PMs from the insects and the application of heparinases (bacterial lyase enzymes that are specific for heparan sulphate (HS) GAG polysaccharides), dot blots with a HS-specific antibody showed heparan sulphate proteoglycans (HSPGs) to be present, consistent with Glossina morsitans morsitans genome analysis, as well as the likely expression of the HSPGs syndecan and perlecan.
29562674	10	66	theme	fine	2222:2225	arg1	structure					2227:2235	HS fine structure	2219:2235	HS fine structure	2219:2235	The presence of significant quantities of disaccharides containing the monosaccharide GlcNAc6S contrasts with previous findings in Drosophila melanogaster and suggests subtle differences in HS fine structure between species of the Diptera.
29562674	10	67	theme	disaccharides	2071:2083	arg1	quantities					2057:2066	significant quantities	2045:2066	significant quantities of disaccharides containing the monosaccharide GlcNAc6S	2045:2122	The presence of significant quantities of disaccharides containing the monosaccharide GlcNAc6S contrasts with previous findings in Drosophila melanogaster and suggests subtle differences in HS fine structure between species of the Diptera.
29562674	3	68	theme	peritrophic	507:517	arg1	matrix					519:524	the insect peritrophic matrix	496:524	the insect peritrophic matrix (PM)	496:529	Precise details of the life cycle have yet to be established, but the parasite life cycle involves crossing the insect peritrophic matrix (PM).
29562674	3	68	theme	peritrophic	507:517	arg1	PM					527:528	PM	527:528	PM	527:528	Precise details of the life cycle have yet to be established, but the parasite life cycle involves crossing the insect peritrophic matrix (PM).
29562674	1	69	theme	sickness	199:206	arg1	vectors					158:164	the principal insect vectors	137:164	the principal insect vectors of African trypanosomes-sleeping sickness in humans and Nagana in cattle	137:237	Tsetse flies are the principal insect vectors of African trypanosomes-sleeping sickness in humans and Nagana in cattle.
29562674	1	69	theme	sickness	199:206	arg1	flies					127:131	Tsetse flies	120:131	Tsetse flies	120:131	Tsetse flies are the principal insect vectors of African trypanosomes-sleeping sickness in humans and Nagana in cattle.
29562674	6	70	with	consistent	1107:1116	arg1	analysis					1159:1166	Glossina morsitans morsitans genome analysis	1123:1166	Glossina morsitans morsitans genome analysis	1123:1166	Following removal of PMs from the insects and the application of heparinases (bacterial lyase enzymes that are specific for heparan sulphate (HS) GAG polysaccharides), dot blots with a HS-specific antibody showed heparan sulphate proteoglycans (HSPGs) to be present, consistent with Glossina morsitans morsitans genome analysis, as well as the likely expression of the HSPGs syndecan and perlecan.
29562674	6	70	with	consistent	1107:1116	arg1	expression					1191:1200	the likely expression	1180:1200	the likely expression of the HSPGs syndecan and perlecan	1180:1235	Following removal of PMs from the insects and the application of heparinases (bacterial lyase enzymes that are specific for heparan sulphate (HS) GAG polysaccharides), dot blots with a HS-specific antibody showed heparan sulphate proteoglycans (HSPGs) to be present, consistent with Glossina morsitans morsitans genome analysis, as well as the likely expression of the HSPGs syndecan and perlecan.
29562674	10	71	theme	Diptera	2260:2266	arg1	species					2245:2251	species	2245:2251	species of the Diptera	2245:2266	The presence of significant quantities of disaccharides containing the monosaccharide GlcNAc6S contrasts with previous findings in Drosophila melanogaster and suggests subtle differences in HS fine structure between species of the Diptera.
29562674	7	72	theme	BODIPY	1337:1342	arg1	fluorophore					1344:1354	BODIPY fluorophore	1337:1354	BODIPY fluorophore	1337:1354	Exhaustive HS digestion with heparinases, fluorescent labeling of the resulting disaccharides with BODIPY fluorophore, and separation by strong anion exchange chromatography then demonstrated the presence of HS for the first time and provided the disaccharide composition.
29562674	9	73	theme	overall	1846:1852	arg1	levels					1854:1859	overall levels	1846:1859	overall levels of 2-O-sulphation	1846:1877	Significant differences, which may relate to differences in infection between genders or ages, were evident, however, in overall levels of 2-O-sulphation between sexes and, for females, between 24 and 48 h post-emergence, implying a change in expression or activity for the 2-O-sulphotransferase enzyme.
29562674	10	74	theme	significant	2045:2055	arg1	quantities					2057:2066	significant quantities	2045:2066	significant quantities of disaccharides containing the monosaccharide GlcNAc6S	2045:2122	The presence of significant quantities of disaccharides containing the monosaccharide GlcNAc6S contrasts with previous findings in Drosophila melanogaster and suggests subtle differences in HS fine structure between species of the Diptera.
29562674	10	75	contain	containing	2085:2094	arg1	disaccharides					2071:2083	disaccharides	2071:2083	disaccharides containing the monosaccharide GlcNAc6S	2071:2122	The presence of significant quantities of disaccharides containing the monosaccharide GlcNAc6S contrasts with previous findings in Drosophila melanogaster and suggests subtle differences in HS fine structure between species of the Diptera.
29562674	10	75	contain	containing	2085:2094	arg2	GlcNAc6S					2115:2122	the monosaccharide GlcNAc6S	2096:2122	the monosaccharide GlcNAc6S	2096:2122	The presence of significant quantities of disaccharides containing the monosaccharide GlcNAc6S contrasts with previous findings in Drosophila melanogaster and suggests subtle differences in HS fine structure between species of the Diptera.
29562674	7	76	theme	disaccharides	1318:1330	arg1	separation					1361:1370	separation	1361:1370	separation by strong anion exchange chromatography	1361:1410	Exhaustive HS digestion with heparinases, fluorescent labeling of the resulting disaccharides with BODIPY fluorophore, and separation by strong anion exchange chromatography then demonstrated the presence of HS for the first time and provided the disaccharide composition.
29562674	7	76	theme	disaccharides	1318:1330	arg1	labeling					1292:1299	fluorescent labeling	1280:1299	fluorescent labeling of the resulting disaccharides with BODIPY fluorophore	1280:1354	Exhaustive HS digestion with heparinases, fluorescent labeling of the resulting disaccharides with BODIPY fluorophore, and separation by strong anion exchange chromatography then demonstrated the presence of HS for the first time and provided the disaccharide composition.
29562674	7	76	theme	disaccharides	1318:1330	arg1	digestion					1252:1260	Exhaustive HS digestion	1238:1260	Exhaustive HS digestion with heparinases	1238:1277	Exhaustive HS digestion with heparinases, fluorescent labeling of the resulting disaccharides with BODIPY fluorophore, and separation by strong anion exchange chromatography then demonstrated the presence of HS for the first time and provided the disaccharide composition.
29562674	4	77	theme	several	578:584	arg1	proteins					594:601	several hundred proteins	578:601	several hundred proteins	578:601	The PM consists of the polysaccharide chitin, several hundred proteins, and both glycosamino- and galactosaminoglycan (GAG) polysaccharides.
29562674	6	78	theme	genome	1152:1157	arg1	analysis					1159:1166	Glossina morsitans morsitans genome analysis	1123:1166	Glossina morsitans morsitans genome analysis	1123:1166	Following removal of PMs from the insects and the application of heparinases (bacterial lyase enzymes that are specific for heparan sulphate (HS) GAG polysaccharides), dot blots with a HS-specific antibody showed heparan sulphate proteoglycans (HSPGs) to be present, consistent with Glossina morsitans morsitans genome analysis, as well as the likely expression of the HSPGs syndecan and perlecan.
29562674	7	79	with	digestion	1252:1260	arg1	fluorophore					1344:1354	BODIPY fluorophore	1337:1354	BODIPY fluorophore	1337:1354	Exhaustive HS digestion with heparinases, fluorescent labeling of the resulting disaccharides with BODIPY fluorophore, and separation by strong anion exchange chromatography then demonstrated the presence of HS for the first time and provided the disaccharide composition.
29562674	7	79	with	digestion	1252:1260	arg1	heparinases					1267:1277	heparinases	1267:1277	heparinases	1267:1277	Exhaustive HS digestion with heparinases, fluorescent labeling of the resulting disaccharides with BODIPY fluorophore, and separation by strong anion exchange chromatography then demonstrated the presence of HS for the first time and provided the disaccharide composition.
29562674	2	80	theme	tsetse	251:256	arg1	fly					258:260	the tsetse fly species	247:268	the tsetse fly species	247:268	One of the tsetse fly species, Glossina morsitans morsitans, is host to the parasite, Trypanosoma brucei, a major cause of African trypanosomiasis.
29562674	1	81	theme	Tsetse	120:125	arg1	vectors					158:164	the principal insect vectors	137:164	the principal insect vectors of African trypanosomes-sleeping sickness in humans and Nagana in cattle	137:237	Tsetse flies are the principal insect vectors of African trypanosomes-sleeping sickness in humans and Nagana in cattle.
29562674	1	81	theme	Tsetse	120:125	arg1	flies					127:131	Tsetse flies	120:131	Tsetse flies	120:131	Tsetse flies are the principal insect vectors of African trypanosomes-sleeping sickness in humans and Nagana in cattle.
29562674	8	82	dep	ages	1620:1623	arg1	h					1636:1636	24 vs. 48 h	1626:1636	24 vs. 48 h post emergence	1626:1651	There were no significant differences in the type of disaccharide species present between genders or between ages (24 vs. 48 h post emergence), although the HS from female flies was more heavily sulphated overall.
29562674	10	83	from	findings	2148:2155	arg1	melanogaster					2171:2182	Drosophila melanogaster	2160:2182	Drosophila melanogaster	2160:2182	The presence of significant quantities of disaccharides containing the monosaccharide GlcNAc6S contrasts with previous findings in Drosophila melanogaster and suggests subtle differences in HS fine structure between species of the Diptera.
29562674	6	84	theme	heparan	964:970	arg1	HS					982:983	HS	982:983	HS	982:983	Following removal of PMs from the insects and the application of heparinases (bacterial lyase enzymes that are specific for heparan sulphate (HS) GAG polysaccharides), dot blots with a HS-specific antibody showed heparan sulphate proteoglycans (HSPGs) to be present, consistent with Glossina morsitans morsitans genome analysis, as well as the likely expression of the HSPGs syndecan and perlecan.
29562674	6	84	theme	heparan	964:970	arg1	sulphate					972:979	heparan sulphate	964:979	heparan sulphate (HS) GAG polysaccharides	964:1004	Following removal of PMs from the insects and the application of heparinases (bacterial lyase enzymes that are specific for heparan sulphate (HS) GAG polysaccharides), dot blots with a HS-specific antibody showed heparan sulphate proteoglycans (HSPGs) to be present, consistent with Glossina morsitans morsitans genome analysis, as well as the likely expression of the HSPGs syndecan and perlecan.
29562674	9	85	from	evident	1825:1831	arg1	levels					1854:1859	overall levels	1846:1859	overall levels of 2-O-sulphation	1846:1877	Significant differences, which may relate to differences in infection between genders or ages, were evident, however, in overall levels of 2-O-sulphation between sexes and, for females, between 24 and 48 h post-emergence, implying a change in expression or activity for the 2-O-sulphotransferase enzyme.
29562674	10	86	theme	monosaccharide	2100:2113	arg1	GlcNAc6S					2115:2122	the monosaccharide GlcNAc6S	2096:2122	the monosaccharide GlcNAc6S	2096:2122	The presence of significant quantities of disaccharides containing the monosaccharide GlcNAc6S contrasts with previous findings in Drosophila melanogaster and suggests subtle differences in HS fine structure between species of the Diptera.
29562674	4	87	theme	glycosamino-	613:624	arg1	polysaccharides					656:670	both glycosamino- and galactosaminoglycan (GAG) polysaccharides	608:670	both glycosamino- and galactosaminoglycan (GAG) polysaccharides	608:670	The PM consists of the polysaccharide chitin, several hundred proteins, and both glycosamino- and galactosaminoglycan (GAG) polysaccharides.
29562674	4	87	theme	glycosamino-	613:624	arg1	GAG					651:653	GAG	651:653	GAG	651:653	The PM consists of the polysaccharide chitin, several hundred proteins, and both glycosamino- and galactosaminoglycan (GAG) polysaccharides.
29562674	0	88	theme	Tsetse	78:83	arg1	Fly					85:87	the Tsetse Fly	74:87	the Tsetse Fly	74:87	Variations in the Peritrophic Matrix Composition of Heparan Sulphate from the Tsetse Fly, Glossina morsitans morsitans.
29562674	1	89	theme	principal	141:149	arg1	vectors					158:164	the principal insect vectors	137:164	the principal insect vectors of African trypanosomes-sleeping sickness in humans and Nagana in cattle	137:237	Tsetse flies are the principal insect vectors of African trypanosomes-sleeping sickness in humans and Nagana in cattle.
29562674	1	89	theme	principal	141:149	arg1	flies					127:131	Tsetse flies	120:131	Tsetse flies	120:131	Tsetse flies are the principal insect vectors of African trypanosomes-sleeping sickness in humans and Nagana in cattle.
29562674	4	90	theme	galactosaminoglycan	630:648	arg1	polysaccharides					656:670	both glycosamino- and galactosaminoglycan (GAG) polysaccharides	608:670	both glycosamino- and galactosaminoglycan (GAG) polysaccharides	608:670	The PM consists of the polysaccharide chitin, several hundred proteins, and both glycosamino- and galactosaminoglycan (GAG) polysaccharides.
29562674	4	90	theme	galactosaminoglycan	630:648	arg1	GAG					651:653	GAG	651:653	GAG	651:653	The PM consists of the polysaccharide chitin, several hundred proteins, and both glycosamino- and galactosaminoglycan (GAG) polysaccharides.
29562674	9	91	from	change	1958:1963	arg1	activity					1982:1989	activity	1982:1989	activity	1982:1989	Significant differences, which may relate to differences in infection between genders or ages, were evident, however, in overall levels of 2-O-sulphation between sexes and, for females, between 24 and 48 h post-emergence, implying a change in expression or activity for the 2-O-sulphotransferase enzyme.
29562674	9	91	from	change	1958:1963	arg1	expression					1968:1977	expression	1968:1977	expression	1968:1977	Significant differences, which may relate to differences in infection between genders or ages, were evident, however, in overall levels of 2-O-sulphation between sexes and, for females, between 24 and 48 h post-emergence, implying a change in expression or activity for the 2-O-sulphotransferase enzyme.
29562674	7	92	theme	disaccharide	1485:1496	arg1	composition					1498:1508	the disaccharide composition	1481:1508	the disaccharide composition	1481:1508	Exhaustive HS digestion with heparinases, fluorescent labeling of the resulting disaccharides with BODIPY fluorophore, and separation by strong anion exchange chromatography then demonstrated the presence of HS for the first time and provided the disaccharide composition.
29562674	10	93	theme	previous	2139:2146	arg1	findings					2148:2155	previous findings	2139:2155	previous findings in Drosophila melanogaster	2139:2182	The presence of significant quantities of disaccharides containing the monosaccharide GlcNAc6S contrasts with previous findings in Drosophila melanogaster and suggests subtle differences in HS fine structure between species of the Diptera.
29562674	3	94	theme	life	411:414	arg1	cycle					416:420	the life cycle	407:420	the life cycle	407:420	Precise details of the life cycle have yet to be established, but the parasite life cycle involves crossing the insect peritrophic matrix (PM).
29562674	7	95	theme	Exhaustive	1238:1247	arg1	digestion					1252:1260	Exhaustive HS digestion	1238:1260	Exhaustive HS digestion with heparinases	1238:1277	Exhaustive HS digestion with heparinases, fluorescent labeling of the resulting disaccharides with BODIPY fluorophore, and separation by strong anion exchange chromatography then demonstrated the presence of HS for the first time and provided the disaccharide composition.
29562674	9	96	theme	Significant	1725:1735	arg1	differences					1737:1747	Significant differences	1725:1747	Significant differences	1725:1747	Significant differences, which may relate to differences in infection between genders or ages, were evident, however, in overall levels of 2-O-sulphation between sexes and, for females, between 24 and 48 h post-emergence, implying a change in expression or activity for the 2-O-sulphotransferase enzyme.
29562674	10	97	from	differences	2204:2214	arg1	structure					2227:2235	HS fine structure	2219:2235	HS fine structure	2219:2235	The presence of significant quantities of disaccharides containing the monosaccharide GlcNAc6S contrasts with previous findings in Drosophila melanogaster and suggests subtle differences in HS fine structure between species of the Diptera.
29562674	0	98	theme	Peritrophic	18:28	arg1	Composition					37:47	the Peritrophic Matrix Composition	14:47	the Peritrophic Matrix Composition of Heparan Sulphate from the Tsetse Fly	14:87	Variations in the Peritrophic Matrix Composition of Heparan Sulphate from the Tsetse Fly, Glossina morsitans morsitans.
29562674	3	99	theme	Precise	388:394	arg1	details					396:402	Precise details	388:402	Precise details of the life cycle	388:420	Precise details of the life cycle have yet to be established, but the parasite life cycle involves crossing the insect peritrophic matrix (PM).
29562674	2	100	theme	African	363:369	arg1	trypanosomiasis					371:385	African trypanosomiasis	363:385	African trypanosomiasis	363:385	One of the tsetse fly species, Glossina morsitans morsitans, is host to the parasite, Trypanosoma brucei, a major cause of African trypanosomiasis.
29562674	0	101	theme	Heparan	52:58	arg1	Sulphate					60:67	Heparan Sulphate	52:67	Heparan Sulphate	52:67	Variations in the Peritrophic Matrix Composition of Heparan Sulphate from the Tsetse Fly, Glossina morsitans morsitans.
29562674	7	102	theme	first	1457:1461	arg1	time					1463:1466	the first time	1453:1466	the first time	1453:1466	Exhaustive HS digestion with heparinases, fluorescent labeling of the resulting disaccharides with BODIPY fluorophore, and separation by strong anion exchange chromatography then demonstrated the presence of HS for the first time and provided the disaccharide composition.
29562674	9	103	from	levels	1854:1859	arg1	evident					1825:1831	evident	1825:1831	evident	1825:1831	Significant differences, which may relate to differences in infection between genders or ages, were evident, however, in overall levels of 2-O-sulphation between sexes and, for females, between 24 and 48 h post-emergence, implying a change in expression or activity for the 2-O-sulphotransferase enzyme.
29562674	6	104	theme	heparan	1053:1059	arg1	HSPGs					1085:1089	HSPGs	1085:1089	HSPGs	1085:1089	Following removal of PMs from the insects and the application of heparinases (bacterial lyase enzymes that are specific for heparan sulphate (HS) GAG polysaccharides), dot blots with a HS-specific antibody showed heparan sulphate proteoglycans (HSPGs) to be present, consistent with Glossina morsitans morsitans genome analysis, as well as the likely expression of the HSPGs syndecan and perlecan.
29562674	6	104	theme	heparan	1053:1059	arg1	proteoglycans					1070:1082	heparan sulphate proteoglycans	1053:1082	heparan sulphate proteoglycans (HSPGs)	1053:1090	Following removal of PMs from the insects and the application of heparinases (bacterial lyase enzymes that are specific for heparan sulphate (HS) GAG polysaccharides), dot blots with a HS-specific antibody showed heparan sulphate proteoglycans (HSPGs) to be present, consistent with Glossina morsitans morsitans genome analysis, as well as the likely expression of the HSPGs syndecan and perlecan.
29562674	8	105	theme	female	1676:1681	arg1	flies					1683:1687	female flies	1676:1687	female flies	1676:1687	There were no significant differences in the type of disaccharide species present between genders or between ages (24 vs. 48 h post emergence), although the HS from female flies was more heavily sulphated overall.
29562674	4	106	theme	polysaccharide	555:568	arg1	chitin					570:575	the polysaccharide chitin	551:575	the polysaccharide chitin	551:575	The PM consists of the polysaccharide chitin, several hundred proteins, and both glycosamino- and galactosaminoglycan (GAG) polysaccharides.
31556290	0	0	theme	Sulfate-Induced	181:195	arg1	Colitis					197:203	Dextran Sodium Sulfate-Induced Colitis	166:203	Dextran Sodium Sulfate-Induced Colitis in Mice	166:211	2-O-β-d-Glucopyranosyl-l-ascorbic Acid, an Ascorbic Acid Derivative Isolated from the Fruits of Lycium Barbarum L., Modulates Gut Microbiota and Palliates Colitis in Dextran Sodium Sulfate-Induced Colitis in Mice.
31556290	3	1	theme	microbiota	961:970	arg1	composition					938:948	the composition	934:948	the composition of the gut microbiota	934:970	Additionally, AA-2βG treatment could promote the production of short-chain fatty acids and modulate the composition of the gut microbiota.
31556290	2	2	theme	tight	809:813	arg1	proteins					824:831	tight junction proteins	809:831	tight junction proteins	809:831	The results revealed that AA-2βG had palliating effects on DSS-induced inflammatory bowel disease (IBD) in terms of slowing down the trends of body weight and solid fecal mass loss, reducing colitis disease activity index, improving serum physiological and biochemical indicators, increasing colon length, blocking proinflammatory cytokines, and increasing tight junction proteins.
31556290	0	3	theme	Sodium	174:179	arg1	Colitis					197:203	Dextran Sodium Sulfate-Induced Colitis	166:203	Dextran Sodium Sulfate-Induced Colitis in Mice	166:211	2-O-β-d-Glucopyranosyl-l-ascorbic Acid, an Ascorbic Acid Derivative Isolated from the Fruits of Lycium Barbarum L., Modulates Gut Microbiota and Palliates Colitis in Dextran Sodium Sulfate-Induced Colitis in Mice.
31556290	1	4	theme	-induced	408:415	arg1	colitis					417:423	the dextran sulfate sodium (DSS)-induced colitis	376:423	the dextran sulfate sodium (DSS)-induced colitis in mice	376:431	In this study, the effects of 2-O-β-d-glucopyranosyl-l-ascorbic acid (AA-2βG), a natural ascorbic acid derivative from the fruits of Lycium barbarum, on treating the dextran sulfate sodium (DSS)-induced colitis in mice were investigated.
31556290	1	5	from	colitis	417:423	arg1	mice					428:431	mice	428:431	mice	428:431	In this study, the effects of 2-O-β-d-glucopyranosyl-l-ascorbic acid (AA-2βG), a natural ascorbic acid derivative from the fruits of Lycium barbarum, on treating the dextran sulfate sodium (DSS)-induced colitis in mice were investigated.
31556290	3	6	theme	AA-2βG	848:853	arg1	treatment					855:863	AA-2βG treatment	848:863	AA-2βG treatment	848:863	Additionally, AA-2βG treatment could promote the production of short-chain fatty acids and modulate the composition of the gut microbiota.
31556290	1	7	theme	2-O-β-d-glucopyranosyl-l-ascorbic	244:276	arg1	AA-2βG					284:289	AA-2βG	284:289	AA-2βG	284:289	In this study, the effects of 2-O-β-d-glucopyranosyl-l-ascorbic acid (AA-2βG), a natural ascorbic acid derivative from the fruits of Lycium barbarum, on treating the dextran sulfate sodium (DSS)-induced colitis in mice were investigated.
31556290	1	7	theme	2-O-β-d-glucopyranosyl-l-ascorbic	244:276	arg1	acid					278:281	2-O-β-d-glucopyranosyl-l-ascorbic acid	244:281	2-O-β-d-glucopyranosyl-l-ascorbic acid (AA-2βG)	244:290	In this study, the effects of 2-O-β-d-glucopyranosyl-l-ascorbic acid (AA-2βG), a natural ascorbic acid derivative from the fruits of Lycium barbarum, on treating the dextran sulfate sodium (DSS)-induced colitis in mice were investigated.
31556290	1	7	theme	2-O-β-d-glucopyranosyl-l-ascorbic	244:276	arg1	derivative					317:326	a natural ascorbic acid derivative	293:326	a natural ascorbic acid derivative from the fruits of Lycium barbarum	293:361	In this study, the effects of 2-O-β-d-glucopyranosyl-l-ascorbic acid (AA-2βG), a natural ascorbic acid derivative from the fruits of Lycium barbarum, on treating the dextran sulfate sodium (DSS)-induced colitis in mice were investigated.
31556290	2	8	theme	solid	611:615	arg1	loss					628:631	solid fecal mass loss	611:631	solid fecal mass loss	611:631	The results revealed that AA-2βG had palliating effects on DSS-induced inflammatory bowel disease (IBD) in terms of slowing down the trends of body weight and solid fecal mass loss, reducing colitis disease activity index, improving serum physiological and biochemical indicators, increasing colon length, blocking proinflammatory cytokines, and increasing tight junction proteins.
31556290	5	9	theme	IBD	1311:1313	arg1	treatment					1298:1306	the treatment	1294:1306	the treatment of IBD	1294:1313	The results indicated that AA-2βG might treat IBD through the regulation of gut microbiota, suggesting that AA-2βG has the potential to be used as a dietary supplement in the treatment of IBD.
31556290	0	10	from	Colitis	155:161	arg1	Colitis					197:203	Dextran Sodium Sulfate-Induced Colitis	166:203	Dextran Sodium Sulfate-Induced Colitis in Mice	166:211	2-O-β-d-Glucopyranosyl-l-ascorbic Acid, an Ascorbic Acid Derivative Isolated from the Fruits of Lycium Barbarum L., Modulates Gut Microbiota and Palliates Colitis in Dextran Sodium Sulfate-Induced Colitis in Mice.
31556290	0	11	theme	Lycium	96:101	arg1	Fruits					86:91	the Fruits	82:91	the Fruits of Lycium Barbarum L.	82:113	2-O-β-d-Glucopyranosyl-l-ascorbic Acid, an Ascorbic Acid Derivative Isolated from the Fruits of Lycium Barbarum L., Modulates Gut Microbiota and Palliates Colitis in Dextran Sodium Sulfate-Induced Colitis in Mice.
31556290	3	12	theme	fatty	909:913	arg1	acids					915:919	short-chain fatty acids	897:919	short-chain fatty acids	897:919	Additionally, AA-2βG treatment could promote the production of short-chain fatty acids and modulate the composition of the gut microbiota.
31556290	4	13	theme	key	977:979	arg1	Prevotellaceae					1042:1055	Prevotellaceae	1042:1055	Prevotellaceae	1042:1055	The key bacteria related to IBD were found to be Porphyromonadaceae, Prevotellaceae, Rikenellaceae, Parasutterella, Parabacteroides, and Clostridium.
31556290	4	13	theme	key	977:979	arg1	bacteria					981:988	The key bacteria	973:988	The key bacteria related to IBD	973:1003	The key bacteria related to IBD were found to be Porphyromonadaceae, Prevotellaceae, Rikenellaceae, Parasutterella, Parabacteroides, and Clostridium.
31556290	5	14	theme	microbiota	1203:1212	arg1	regulation					1185:1194	the regulation	1181:1194	the regulation of gut microbiota	1181:1212	The results indicated that AA-2βG might treat IBD through the regulation of gut microbiota, suggesting that AA-2βG has the potential to be used as a dietary supplement in the treatment of IBD.
31556290	1	15	theme	barbarum	354:361	arg1	fruits					337:342	the fruits	333:342	the fruits of Lycium barbarum	333:361	In this study, the effects of 2-O-β-d-glucopyranosyl-l-ascorbic acid (AA-2βG), a natural ascorbic acid derivative from the fruits of Lycium barbarum, on treating the dextran sulfate sodium (DSS)-induced colitis in mice were investigated.
31556290	2	16	theme	body	595:598	arg1	weight					600:605	body weight	595:605	body weight	595:605	The results revealed that AA-2βG had palliating effects on DSS-induced inflammatory bowel disease (IBD) in terms of slowing down the trends of body weight and solid fecal mass loss, reducing colitis disease activity index, improving serum physiological and biochemical indicators, increasing colon length, blocking proinflammatory cytokines, and increasing tight junction proteins.
31556290	2	17	theme	weight	600:605	arg1	trends					585:590	the trends	581:590	the trends of body weight and solid fecal mass loss	581:631	The results revealed that AA-2βG had palliating effects on DSS-induced inflammatory bowel disease (IBD) in terms of slowing down the trends of body weight and solid fecal mass loss, reducing colitis disease activity index, improving serum physiological and biochemical indicators, increasing colon length, blocking proinflammatory cytokines, and increasing tight junction proteins.
31556290	3	18	theme	short-chain	897:907	arg1	acids					915:919	short-chain fatty acids	897:919	short-chain fatty acids	897:919	Additionally, AA-2βG treatment could promote the production of short-chain fatty acids and modulate the composition of the gut microbiota.
31556290	0	19	theme	Acid	52:55	arg1	Derivative					57:66	an Ascorbic Acid Derivative	40:66	an Ascorbic Acid Derivative Isolated from the Fruits of Lycium Barbarum L.	40:113	2-O-β-d-Glucopyranosyl-l-ascorbic Acid, an Ascorbic Acid Derivative Isolated from the Fruits of Lycium Barbarum L., Modulates Gut Microbiota and Palliates Colitis in Dextran Sodium Sulfate-Induced Colitis in Mice.
31556290	0	19	theme	Acid	52:55	arg1	Acid					34:37	2-O-β-d-Glucopyranosyl-l-ascorbic Acid	0:37	2-O-β-d-Glucopyranosyl-l-ascorbic Acid	0:37	2-O-β-d-Glucopyranosyl-l-ascorbic Acid, an Ascorbic Acid Derivative Isolated from the Fruits of Lycium Barbarum L., Modulates Gut Microbiota and Palliates Colitis in Dextran Sodium Sulfate-Induced Colitis in Mice.
31556290	0	20	dep	Lycium	96:101	arg1	L.					112:113	Lycium Barbarum L.	96:113	Lycium Barbarum L.	96:113	2-O-β-d-Glucopyranosyl-l-ascorbic Acid, an Ascorbic Acid Derivative Isolated from the Fruits of Lycium Barbarum L., Modulates Gut Microbiota and Palliates Colitis in Dextran Sodium Sulfate-Induced Colitis in Mice.
31556290	2	21	theme	biochemical	709:719	arg1	indicators					721:730	serum physiological and biochemical indicators	685:730	serum physiological and biochemical indicators	685:730	The results revealed that AA-2βG had palliating effects on DSS-induced inflammatory bowel disease (IBD) in terms of slowing down the trends of body weight and solid fecal mass loss, reducing colitis disease activity index, improving serum physiological and biochemical indicators, increasing colon length, blocking proinflammatory cytokines, and increasing tight junction proteins.
31556290	0	22	theme	2-O-β-d-Glucopyranosyl-l-ascorbic	0:32	arg1	Derivative					57:66	an Ascorbic Acid Derivative	40:66	an Ascorbic Acid Derivative Isolated from the Fruits of Lycium Barbarum L.	40:113	2-O-β-d-Glucopyranosyl-l-ascorbic Acid, an Ascorbic Acid Derivative Isolated from the Fruits of Lycium Barbarum L., Modulates Gut Microbiota and Palliates Colitis in Dextran Sodium Sulfate-Induced Colitis in Mice.
31556290	0	22	theme	2-O-β-d-Glucopyranosyl-l-ascorbic	0:32	arg1	Acid					34:37	2-O-β-d-Glucopyranosyl-l-ascorbic Acid	0:37	2-O-β-d-Glucopyranosyl-l-ascorbic Acid	0:37	2-O-β-d-Glucopyranosyl-l-ascorbic Acid, an Ascorbic Acid Derivative Isolated from the Fruits of Lycium Barbarum L., Modulates Gut Microbiota and Palliates Colitis in Dextran Sodium Sulfate-Induced Colitis in Mice.
31556290	2	23	theme	loss	628:631	arg1	trends					585:590	the trends	581:590	the trends of body weight and solid fecal mass loss	581:631	The results revealed that AA-2βG had palliating effects on DSS-induced inflammatory bowel disease (IBD) in terms of slowing down the trends of body weight and solid fecal mass loss, reducing colitis disease activity index, improving serum physiological and biochemical indicators, increasing colon length, blocking proinflammatory cytokines, and increasing tight junction proteins.
31556290	5	24	theme	dietary	1272:1278	arg1	supplement					1280:1289	a dietary supplement	1270:1289	a dietary supplement in the treatment of IBD	1270:1313	The results indicated that AA-2βG might treat IBD through the regulation of gut microbiota, suggesting that AA-2βG has the potential to be used as a dietary supplement in the treatment of IBD.
31556290	0	25	from	Colitis	197:203	arg1	Mice					208:211	Mice	208:211	Mice	208:211	2-O-β-d-Glucopyranosyl-l-ascorbic Acid, an Ascorbic Acid Derivative Isolated from the Fruits of Lycium Barbarum L., Modulates Gut Microbiota and Palliates Colitis in Dextran Sodium Sulfate-Induced Colitis in Mice.
31556290	0	26	theme	Gut	126:128	arg1	Microbiota					130:139	Gut Microbiota	126:139	Gut Microbiota	126:139	2-O-β-d-Glucopyranosyl-l-ascorbic Acid, an Ascorbic Acid Derivative Isolated from the Fruits of Lycium Barbarum L., Modulates Gut Microbiota and Palliates Colitis in Dextran Sodium Sulfate-Induced Colitis in Mice.
31556290	0	27	attach	Isolated	68:75	arg1	Fruits					86:91	the Fruits	82:91	the Fruits of Lycium Barbarum L.	82:113	2-O-β-d-Glucopyranosyl-l-ascorbic Acid, an Ascorbic Acid Derivative Isolated from the Fruits of Lycium Barbarum L., Modulates Gut Microbiota and Palliates Colitis in Dextran Sodium Sulfate-Induced Colitis in Mice.
31556290	0	27	attach	Isolated	68:75	arg2	Derivative					57:66	an Ascorbic Acid Derivative	40:66	an Ascorbic Acid Derivative Isolated from the Fruits of Lycium Barbarum L.	40:113	2-O-β-d-Glucopyranosyl-l-ascorbic Acid, an Ascorbic Acid Derivative Isolated from the Fruits of Lycium Barbarum L., Modulates Gut Microbiota and Palliates Colitis in Dextran Sodium Sulfate-Induced Colitis in Mice.
31556290	0	27	attach	Isolated	68:75	arg2	Acid					34:37	2-O-β-d-Glucopyranosyl-l-ascorbic Acid	0:37	2-O-β-d-Glucopyranosyl-l-ascorbic Acid	0:37	2-O-β-d-Glucopyranosyl-l-ascorbic Acid, an Ascorbic Acid Derivative Isolated from the Fruits of Lycium Barbarum L., Modulates Gut Microbiota and Palliates Colitis in Dextran Sodium Sulfate-Induced Colitis in Mice.
31556290	2	28	theme	fecal	617:621	arg1	loss					628:631	solid fecal mass loss	611:631	solid fecal mass loss	611:631	The results revealed that AA-2βG had palliating effects on DSS-induced inflammatory bowel disease (IBD) in terms of slowing down the trends of body weight and solid fecal mass loss, reducing colitis disease activity index, improving serum physiological and biochemical indicators, increasing colon length, blocking proinflammatory cytokines, and increasing tight junction proteins.
31556290	2	29	theme	bowel	536:540	arg1	IBD					551:553	IBD	551:553	IBD	551:553	The results revealed that AA-2βG had palliating effects on DSS-induced inflammatory bowel disease (IBD) in terms of slowing down the trends of body weight and solid fecal mass loss, reducing colitis disease activity index, improving serum physiological and biochemical indicators, increasing colon length, blocking proinflammatory cytokines, and increasing tight junction proteins.
31556290	2	29	theme	bowel	536:540	arg1	disease					542:548	DSS-induced inflammatory bowel disease	511:548	DSS-induced inflammatory bowel disease (IBD) in terms of slowing down the trends of body weight and solid fecal mass loss	511:631	The results revealed that AA-2βG had palliating effects on DSS-induced inflammatory bowel disease (IBD) in terms of slowing down the trends of body weight and solid fecal mass loss, reducing colitis disease activity index, improving serum physiological and biochemical indicators, increasing colon length, blocking proinflammatory cytokines, and increasing tight junction proteins.
31556290	2	30	theme	colon	744:748	arg1	length					750:755	colon length	744:755	colon length	744:755	The results revealed that AA-2βG had palliating effects on DSS-induced inflammatory bowel disease (IBD) in terms of slowing down the trends of body weight and solid fecal mass loss, reducing colitis disease activity index, improving serum physiological and biochemical indicators, increasing colon length, blocking proinflammatory cytokines, and increasing tight junction proteins.
31556290	1	31	from	fruits	337:342	arg1	acid					278:281	2-O-β-d-glucopyranosyl-l-ascorbic acid	244:281	2-O-β-d-glucopyranosyl-l-ascorbic acid (AA-2βG)	244:290	In this study, the effects of 2-O-β-d-glucopyranosyl-l-ascorbic acid (AA-2βG), a natural ascorbic acid derivative from the fruits of Lycium barbarum, on treating the dextran sulfate sodium (DSS)-induced colitis in mice were investigated.
31556290	1	31	from	fruits	337:342	arg1	derivative					317:326	a natural ascorbic acid derivative	293:326	a natural ascorbic acid derivative from the fruits of Lycium barbarum	293:361	In this study, the effects of 2-O-β-d-glucopyranosyl-l-ascorbic acid (AA-2βG), a natural ascorbic acid derivative from the fruits of Lycium barbarum, on treating the dextran sulfate sodium (DSS)-induced colitis in mice were investigated.
31556290	2	32	theme	inflammatory	523:534	arg1	IBD					551:553	IBD	551:553	IBD	551:553	The results revealed that AA-2βG had palliating effects on DSS-induced inflammatory bowel disease (IBD) in terms of slowing down the trends of body weight and solid fecal mass loss, reducing colitis disease activity index, improving serum physiological and biochemical indicators, increasing colon length, blocking proinflammatory cytokines, and increasing tight junction proteins.
31556290	2	32	theme	inflammatory	523:534	arg1	disease					542:548	DSS-induced inflammatory bowel disease	511:548	DSS-induced inflammatory bowel disease (IBD) in terms of slowing down the trends of body weight and solid fecal mass loss	511:631	The results revealed that AA-2βG had palliating effects on DSS-induced inflammatory bowel disease (IBD) in terms of slowing down the trends of body weight and solid fecal mass loss, reducing colitis disease activity index, improving serum physiological and biochemical indicators, increasing colon length, blocking proinflammatory cytokines, and increasing tight junction proteins.
31556290	3	33	theme	acids	915:919	arg1	production					883:892	the production	879:892	the production of short-chain fatty acids	879:919	Additionally, AA-2βG treatment could promote the production of short-chain fatty acids and modulate the composition of the gut microbiota.
31556290	0	34	theme	Ascorbic	43:50	arg1	Derivative					57:66	an Ascorbic Acid Derivative	40:66	an Ascorbic Acid Derivative Isolated from the Fruits of Lycium Barbarum L.	40:113	2-O-β-d-Glucopyranosyl-l-ascorbic Acid, an Ascorbic Acid Derivative Isolated from the Fruits of Lycium Barbarum L., Modulates Gut Microbiota and Palliates Colitis in Dextran Sodium Sulfate-Induced Colitis in Mice.
31556290	0	34	theme	Ascorbic	43:50	arg1	Acid					34:37	2-O-β-d-Glucopyranosyl-l-ascorbic Acid	0:37	2-O-β-d-Glucopyranosyl-l-ascorbic Acid	0:37	2-O-β-d-Glucopyranosyl-l-ascorbic Acid, an Ascorbic Acid Derivative Isolated from the Fruits of Lycium Barbarum L., Modulates Gut Microbiota and Palliates Colitis in Dextran Sodium Sulfate-Induced Colitis in Mice.
31556290	1	35	theme	natural	295:301	arg1	acid					278:281	2-O-β-d-glucopyranosyl-l-ascorbic acid	244:281	2-O-β-d-glucopyranosyl-l-ascorbic acid (AA-2βG)	244:290	In this study, the effects of 2-O-β-d-glucopyranosyl-l-ascorbic acid (AA-2βG), a natural ascorbic acid derivative from the fruits of Lycium barbarum, on treating the dextran sulfate sodium (DSS)-induced colitis in mice were investigated.
31556290	1	35	theme	natural	295:301	arg1	derivative					317:326	a natural ascorbic acid derivative	293:326	a natural ascorbic acid derivative from the fruits of Lycium barbarum	293:361	In this study, the effects of 2-O-β-d-glucopyranosyl-l-ascorbic acid (AA-2βG), a natural ascorbic acid derivative from the fruits of Lycium barbarum, on treating the dextran sulfate sodium (DSS)-induced colitis in mice were investigated.
31556290	2	36	theme	DSS-induced	511:521	arg1	IBD					551:553	IBD	551:553	IBD	551:553	The results revealed that AA-2βG had palliating effects on DSS-induced inflammatory bowel disease (IBD) in terms of slowing down the trends of body weight and solid fecal mass loss, reducing colitis disease activity index, improving serum physiological and biochemical indicators, increasing colon length, blocking proinflammatory cytokines, and increasing tight junction proteins.
31556290	2	36	theme	DSS-induced	511:521	arg1	disease					542:548	DSS-induced inflammatory bowel disease	511:548	DSS-induced inflammatory bowel disease (IBD) in terms of slowing down the trends of body weight and solid fecal mass loss	511:631	The results revealed that AA-2βG had palliating effects on DSS-induced inflammatory bowel disease (IBD) in terms of slowing down the trends of body weight and solid fecal mass loss, reducing colitis disease activity index, improving serum physiological and biochemical indicators, increasing colon length, blocking proinflammatory cytokines, and increasing tight junction proteins.
31556290	1	37	theme	ascorbic	303:310	arg1	acid					278:281	2-O-β-d-glucopyranosyl-l-ascorbic acid	244:281	2-O-β-d-glucopyranosyl-l-ascorbic acid (AA-2βG)	244:290	In this study, the effects of 2-O-β-d-glucopyranosyl-l-ascorbic acid (AA-2βG), a natural ascorbic acid derivative from the fruits of Lycium barbarum, on treating the dextran sulfate sodium (DSS)-induced colitis in mice were investigated.
31556290	1	37	theme	ascorbic	303:310	arg1	derivative					317:326	a natural ascorbic acid derivative	293:326	a natural ascorbic acid derivative from the fruits of Lycium barbarum	293:361	In this study, the effects of 2-O-β-d-glucopyranosyl-l-ascorbic acid (AA-2βG), a natural ascorbic acid derivative from the fruits of Lycium barbarum, on treating the dextran sulfate sodium (DSS)-induced colitis in mice were investigated.
31556290	2	38	theme	activity	659:666	arg1	index					668:672	colitis disease activity index	643:672	colitis disease activity index	643:672	The results revealed that AA-2βG had palliating effects on DSS-induced inflammatory bowel disease (IBD) in terms of slowing down the trends of body weight and solid fecal mass loss, reducing colitis disease activity index, improving serum physiological and biochemical indicators, increasing colon length, blocking proinflammatory cytokines, and increasing tight junction proteins.
31556290	4	39	theme	related	990:996	arg1	Prevotellaceae					1042:1055	Prevotellaceae	1042:1055	Prevotellaceae	1042:1055	The key bacteria related to IBD were found to be Porphyromonadaceae, Prevotellaceae, Rikenellaceae, Parasutterella, Parabacteroides, and Clostridium.
31556290	4	39	theme	related	990:996	arg1	bacteria					981:988	The key bacteria	973:988	The key bacteria related to IBD	973:1003	The key bacteria related to IBD were found to be Porphyromonadaceae, Prevotellaceae, Rikenellaceae, Parasutterella, Parabacteroides, and Clostridium.
31556290	1	40	theme	acid	312:315	arg1	acid					278:281	2-O-β-d-glucopyranosyl-l-ascorbic acid	244:281	2-O-β-d-glucopyranosyl-l-ascorbic acid (AA-2βG)	244:290	In this study, the effects of 2-O-β-d-glucopyranosyl-l-ascorbic acid (AA-2βG), a natural ascorbic acid derivative from the fruits of Lycium barbarum, on treating the dextran sulfate sodium (DSS)-induced colitis in mice were investigated.
31556290	1	40	theme	acid	312:315	arg1	derivative					317:326	a natural ascorbic acid derivative	293:326	a natural ascorbic acid derivative from the fruits of Lycium barbarum	293:361	In this study, the effects of 2-O-β-d-glucopyranosyl-l-ascorbic acid (AA-2βG), a natural ascorbic acid derivative from the fruits of Lycium barbarum, on treating the dextran sulfate sodium (DSS)-induced colitis in mice were investigated.
31556290	0	41	theme	Palliates	145:153	arg1	Colitis					155:161	Palliates Colitis	145:161	Palliates Colitis	145:161	2-O-β-d-Glucopyranosyl-l-ascorbic Acid, an Ascorbic Acid Derivative Isolated from the Fruits of Lycium Barbarum L., Modulates Gut Microbiota and Palliates Colitis in Dextran Sodium Sulfate-Induced Colitis in Mice.
31556290	5	42	from	supplement	1280:1289	arg1	treatment					1298:1306	the treatment	1294:1306	the treatment of IBD	1294:1313	The results indicated that AA-2βG might treat IBD through the regulation of gut microbiota, suggesting that AA-2βG has the potential to be used as a dietary supplement in the treatment of IBD.
31556290	2	43	theme	disease	651:657	arg1	index					668:672	colitis disease activity index	643:672	colitis disease activity index	643:672	The results revealed that AA-2βG had palliating effects on DSS-induced inflammatory bowel disease (IBD) in terms of slowing down the trends of body weight and solid fecal mass loss, reducing colitis disease activity index, improving serum physiological and biochemical indicators, increasing colon length, blocking proinflammatory cytokines, and increasing tight junction proteins.
31556290	2	44	theme	mass	623:626	arg1	loss					628:631	solid fecal mass loss	611:631	solid fecal mass loss	611:631	The results revealed that AA-2βG had palliating effects on DSS-induced inflammatory bowel disease (IBD) in terms of slowing down the trends of body weight and solid fecal mass loss, reducing colitis disease activity index, improving serum physiological and biochemical indicators, increasing colon length, blocking proinflammatory cytokines, and increasing tight junction proteins.
31556290	0	45	theme	Dextran	166:172	arg1	Sodium					174:179	Dextran Sodium	166:179	Dextran Sodium Sulfate-Induced Colitis in Mice	166:211	2-O-β-d-Glucopyranosyl-l-ascorbic Acid, an Ascorbic Acid Derivative Isolated from the Fruits of Lycium Barbarum L., Modulates Gut Microbiota and Palliates Colitis in Dextran Sodium Sulfate-Induced Colitis in Mice.
31556290	5	46	theme	gut	1199:1201	arg1	microbiota					1203:1212	gut microbiota	1199:1212	gut microbiota	1199:1212	The results indicated that AA-2βG might treat IBD through the regulation of gut microbiota, suggesting that AA-2βG has the potential to be used as a dietary supplement in the treatment of IBD.
31556290	2	47	theme	colitis	643:649	arg1	index					668:672	colitis disease activity index	643:672	colitis disease activity index	643:672	The results revealed that AA-2βG had palliating effects on DSS-induced inflammatory bowel disease (IBD) in terms of slowing down the trends of body weight and solid fecal mass loss, reducing colitis disease activity index, improving serum physiological and biochemical indicators, increasing colon length, blocking proinflammatory cytokines, and increasing tight junction proteins.
31556290	2	48	theme	physiological	691:703	arg1	indicators					721:730	serum physiological and biochemical indicators	685:730	serum physiological and biochemical indicators	685:730	The results revealed that AA-2βG had palliating effects on DSS-induced inflammatory bowel disease (IBD) in terms of slowing down the trends of body weight and solid fecal mass loss, reducing colitis disease activity index, improving serum physiological and biochemical indicators, increasing colon length, blocking proinflammatory cytokines, and increasing tight junction proteins.
31556290	2	49	theme	proinflammatory	767:781	arg1	cytokines					783:791	proinflammatory cytokines	767:791	proinflammatory cytokines	767:791	The results revealed that AA-2βG had palliating effects on DSS-induced inflammatory bowel disease (IBD) in terms of slowing down the trends of body weight and solid fecal mass loss, reducing colitis disease activity index, improving serum physiological and biochemical indicators, increasing colon length, blocking proinflammatory cytokines, and increasing tight junction proteins.
31556290	0	50	from	Microbiota	130:139	arg1	Colitis					197:203	Dextran Sodium Sulfate-Induced Colitis	166:203	Dextran Sodium Sulfate-Induced Colitis in Mice	166:211	2-O-β-d-Glucopyranosyl-l-ascorbic Acid, an Ascorbic Acid Derivative Isolated from the Fruits of Lycium Barbarum L., Modulates Gut Microbiota and Palliates Colitis in Dextran Sodium Sulfate-Induced Colitis in Mice.
31556290	2	51	from	effects	500:506	arg1	IBD					551:553	IBD	551:553	IBD	551:553	The results revealed that AA-2βG had palliating effects on DSS-induced inflammatory bowel disease (IBD) in terms of slowing down the trends of body weight and solid fecal mass loss, reducing colitis disease activity index, improving serum physiological and biochemical indicators, increasing colon length, blocking proinflammatory cytokines, and increasing tight junction proteins.
31556290	2	51	from	effects	500:506	arg1	disease					542:548	DSS-induced inflammatory bowel disease	511:548	DSS-induced inflammatory bowel disease (IBD) in terms of slowing down the trends of body weight and solid fecal mass loss	511:631	The results revealed that AA-2βG had palliating effects on DSS-induced inflammatory bowel disease (IBD) in terms of slowing down the trends of body weight and solid fecal mass loss, reducing colitis disease activity index, improving serum physiological and biochemical indicators, increasing colon length, blocking proinflammatory cytokines, and increasing tight junction proteins.
31556290	2	52	theme	serum	685:689	arg1	indicators					721:730	serum physiological and biochemical indicators	685:730	serum physiological and biochemical indicators	685:730	The results revealed that AA-2βG had palliating effects on DSS-induced inflammatory bowel disease (IBD) in terms of slowing down the trends of body weight and solid fecal mass loss, reducing colitis disease activity index, improving serum physiological and biochemical indicators, increasing colon length, blocking proinflammatory cytokines, and increasing tight junction proteins.
31556290	2	53	from	disease	542:548	arg1	terms					559:563	terms	559:563	terms of slowing down the trends of body weight and solid fecal mass loss	559:631	The results revealed that AA-2βG had palliating effects on DSS-induced inflammatory bowel disease (IBD) in terms of slowing down the trends of body weight and solid fecal mass loss, reducing colitis disease activity index, improving serum physiological and biochemical indicators, increasing colon length, blocking proinflammatory cytokines, and increasing tight junction proteins.
31556290	1	54	theme	acid	278:281	arg1	effects					233:239	the effects	229:239	the effects of 2-O-β-d-glucopyranosyl-l-ascorbic acid (AA-2βG), a natural ascorbic acid derivative from the fruits of Lycium barbarum, on treating the dextran sulfate sodium (DSS)-induced colitis in mice	229:431	In this study, the effects of 2-O-β-d-glucopyranosyl-l-ascorbic acid (AA-2βG), a natural ascorbic acid derivative from the fruits of Lycium barbarum, on treating the dextran sulfate sodium (DSS)-induced colitis in mice were investigated.
31556290	5	55	contain	has	1238:1240	arg2	potential					1246:1254	the potential to be used as a dietary supplement in the treatment of IBD	1242:1313	the potential to be used as a dietary supplement in the treatment of IBD	1242:1313	The results indicated that AA-2βG might treat IBD through the regulation of gut microbiota, suggesting that AA-2βG has the potential to be used as a dietary supplement in the treatment of IBD.
31556290	5	55	contain	has	1238:1240	arg1	AA-2βG					1231:1236	AA-2βG	1231:1236	AA-2βG	1231:1236	The results indicated that AA-2βG might treat IBD through the regulation of gut microbiota, suggesting that AA-2βG has the potential to be used as a dietary supplement in the treatment of IBD.
31556290	3	56	theme	gut	957:959	arg1	microbiota					961:970	the gut microbiota	953:970	the gut microbiota	953:970	Additionally, AA-2βG treatment could promote the production of short-chain fatty acids and modulate the composition of the gut microbiota.
31556290	2	57	theme	junction	815:822	arg1	proteins					824:831	tight junction proteins	809:831	tight junction proteins	809:831	The results revealed that AA-2βG had palliating effects on DSS-induced inflammatory bowel disease (IBD) in terms of slowing down the trends of body weight and solid fecal mass loss, reducing colitis disease activity index, improving serum physiological and biochemical indicators, increasing colon length, blocking proinflammatory cytokines, and increasing tight junction proteins.
30737752	4	0	theme	chapter	499:505	arg1	methods					507:513	this chapter methods	494:513	this chapter methods	494:513	Therefore, conventional and sophisticated techniques are available, providing diverse information about the product quality.In this chapter methods are presented, which enable the determination of the overall protein quality, their aggregation and peptide composition.
30737752	1	1	theme	intended	138:145	arg1	purpose					147:153	their intended purpose	132:153	their intended purpose	132:153	The product quality of recombinant proteins is of major importance for their intended purpose.
30737752	6	2	theme	individual	920:929	arg1	strengths					931:939	individual strengths	920:939	individual strengths	920:939	In this regard, chromatographic, high-resolution technologies for the integrity of proteins as well as Western blot with specific detection methods are introduced, and individual strengths and perceived limitations are highlighted.
30737752	4	3	theme	quality	584:590	arg1	determination					547:559	the determination	543:559	the determination of the overall protein quality, their aggregation and peptide composition	543:633	Therefore, conventional and sophisticated techniques are available, providing diverse information about the product quality.In this chapter methods are presented, which enable the determination of the overall protein quality, their aggregation and peptide composition.
30737752	6	4	theme	perceived	945:953	arg1	limitations					955:965	perceived limitations	945:965	perceived limitations	945:965	In this regard, chromatographic, high-resolution technologies for the integrity of proteins as well as Western blot with specific detection methods are introduced, and individual strengths and perceived limitations are highlighted.
30737752	4	5	theme	conventional	378:389	arg1	techniques					409:418	conventional and sophisticated techniques	378:418	conventional and sophisticated techniques	378:418	Therefore, conventional and sophisticated techniques are available, providing diverse information about the product quality.In this chapter methods are presented, which enable the determination of the overall protein quality, their aggregation and peptide composition.
30737752	4	6	theme	protein	576:582	arg1	quality					584:590	the overall protein quality	564:590	the overall protein quality	564:590	Therefore, conventional and sophisticated techniques are available, providing diverse information about the product quality.In this chapter methods are presented, which enable the determination of the overall protein quality, their aggregation and peptide composition.
30737752	4	6	theme	protein	576:582	arg1	aggregation					599:609	their aggregation	593:609	their aggregation	593:609	Therefore, conventional and sophisticated techniques are available, providing diverse information about the product quality.In this chapter methods are presented, which enable the determination of the overall protein quality, their aggregation and peptide composition.
30737752	4	6	theme	protein	576:582	arg1	composition					623:633	peptide composition	615:633	peptide composition	615:633	Therefore, conventional and sophisticated techniques are available, providing diverse information about the product quality.In this chapter methods are presented, which enable the determination of the overall protein quality, their aggregation and peptide composition.
30737752	3	7	theme	complementary	332:344	arg1	methods					346:352	complementary methods	332:352	complementary methods	332:352	However, to comply with this high standard, appropriate selection of complementary methods is required.
30737752	6	8	theme	proteins	835:842	arg1	blot					863:866	Western blot	855:866	the integrity of proteins as well as Western blot	818:866	In this regard, chromatographic, high-resolution technologies for the integrity of proteins as well as Western blot with specific detection methods are introduced, and individual strengths and perceived limitations are highlighted.
30737752	6	8	theme	proteins	835:842	arg1	integrity					822:830	the integrity	818:830	the integrity of proteins as well as Western blot	818:866	In this regard, chromatographic, high-resolution technologies for the integrity of proteins as well as Western blot with specific detection methods are introduced, and individual strengths and perceived limitations are highlighted.
30737752	3	9	theme	methods	346:352	arg1	selection					319:327	appropriate selection	307:327	appropriate selection of complementary methods	307:352	However, to comply with this high standard, appropriate selection of complementary methods is required.
30737752	4	10	theme	product	475:481	arg1	quality.In					483:492	the product quality.In	471:492	the product quality.In	471:492	Therefore, conventional and sophisticated techniques are available, providing diverse information about the product quality.In this chapter methods are presented, which enable the determination of the overall protein quality, their aggregation and peptide composition.
30737752	5	11	theme	posttranslational	677:693	arg1	modifications					695:707	posttranslational modifications	677:707	posttranslational modifications such as glycan analysis	677:731	Methods applied for the determination of posttranslational modifications such as glycan analysis are not described.
30737752	5	11	theme	posttranslational	677:693	arg1	analysis					724:731	glycan analysis	717:731	glycan analysis	717:731	Methods applied for the determination of posttranslational modifications such as glycan analysis are not described.
30737752	4	12	theme	sophisticated	395:407	arg1	techniques					409:418	conventional and sophisticated techniques	378:418	conventional and sophisticated techniques	378:418	Therefore, conventional and sophisticated techniques are available, providing diverse information about the product quality.In this chapter methods are presented, which enable the determination of the overall protein quality, their aggregation and peptide composition.
30737752	4	13	dep	providing	435:443	arg1	presented					519:527	presented	519:527	are presented	515:527	Therefore, conventional and sophisticated techniques are available, providing diverse information about the product quality.In this chapter methods are presented, which enable the determination of the overall protein quality, their aggregation and peptide composition.
30737752	1	14	theme	product	65:71	arg1	quality					73:79	The product quality	61:79	The product quality of recombinant proteins	61:103	The product quality of recombinant proteins is of major importance for their intended purpose.
30737752	1	15	theme	recombinant	84:94	arg1	proteins					96:103	recombinant proteins	84:103	recombinant proteins	84:103	The product quality of recombinant proteins is of major importance for their intended purpose.
30737752	4	16	theme	overall	568:574	arg1	quality					584:590	the overall protein quality	564:590	the overall protein quality	564:590	Therefore, conventional and sophisticated techniques are available, providing diverse information about the product quality.In this chapter methods are presented, which enable the determination of the overall protein quality, their aggregation and peptide composition.
30737752	4	16	theme	overall	568:574	arg1	aggregation					599:609	their aggregation	593:609	their aggregation	593:609	Therefore, conventional and sophisticated techniques are available, providing diverse information about the product quality.In this chapter methods are presented, which enable the determination of the overall protein quality, their aggregation and peptide composition.
30737752	4	16	theme	overall	568:574	arg1	composition					623:633	peptide composition	615:633	peptide composition	615:633	Therefore, conventional and sophisticated techniques are available, providing diverse information about the product quality.In this chapter methods are presented, which enable the determination of the overall protein quality, their aggregation and peptide composition.
30737752	4	17	theme	diverse	445:451	arg1	information					453:463	diverse information	445:463	diverse information about the product quality.In	445:492	Therefore, conventional and sophisticated techniques are available, providing diverse information about the product quality.In this chapter methods are presented, which enable the determination of the overall protein quality, their aggregation and peptide composition.
30737752	1	18	theme	proteins	96:103	arg1	quality					73:79	The product quality	61:79	The product quality of recombinant proteins	61:103	The product quality of recombinant proteins is of major importance for their intended purpose.
30737752	0	19	theme	Products	37:44	arg1	Quality					14:20	the Quality	10:20	the Quality of Recombinant Products Made in Yeast	10:58	Assessing the Quality of Recombinant Products Made in Yeast.
30737752	2	20	theme	initial	160:166	arg1	characterization					168:183	The initial characterization	156:183	The initial characterization of both simple and complex products	156:219	The initial characterization of both simple and complex products should be performed as soon as practical.
30737752	0	21	theme	Recombinant	25:35	arg1	Products					37:44	Recombinant Products	25:44	Recombinant Products Made in Yeast	25:58	Assessing the Quality of Recombinant Products Made in Yeast.
30737752	6	22	with	technologies	801:812	arg1	methods					892:898	specific detection methods	873:898	specific detection methods	873:898	In this regard, chromatographic, high-resolution technologies for the integrity of proteins as well as Western blot with specific detection methods are introduced, and individual strengths and perceived limitations are highlighted.
30737752	6	23	theme	detection	882:890	arg1	methods					892:898	specific detection methods	873:898	specific detection methods	873:898	In this regard, chromatographic, high-resolution technologies for the integrity of proteins as well as Western blot with specific detection methods are introduced, and individual strengths and perceived limitations are highlighted.
30737752	5	24	theme	modifications	695:707	arg1	determination					660:672	the determination	656:672	the determination of posttranslational modifications such as glycan analysis	656:731	Methods applied for the determination of posttranslational modifications such as glycan analysis are not described.
30737752	6	25	theme	specific	873:880	arg1	methods					892:898	specific detection methods	873:898	specific detection methods	873:898	In this regard, chromatographic, high-resolution technologies for the integrity of proteins as well as Western blot with specific detection methods are introduced, and individual strengths and perceived limitations are highlighted.
30737752	2	26	theme	products	212:219	arg1	characterization					168:183	The initial characterization	156:183	The initial characterization of both simple and complex products	156:219	The initial characterization of both simple and complex products should be performed as soon as practical.
30737752	2	27	theme	complex	204:210	arg1	products					212:219	both simple and complex products	188:219	both simple and complex products	188:219	The initial characterization of both simple and complex products should be performed as soon as practical.
30737752	6	28	theme	chromatographic	768:782	arg1	technologies					801:812	chromatographic, high-resolution technologies	768:812	chromatographic, high-resolution technologies for the integrity of proteins as well as Western blot with specific detection methods	768:898	In this regard, chromatographic, high-resolution technologies for the integrity of proteins as well as Western blot with specific detection methods are introduced, and individual strengths and perceived limitations are highlighted.
30737752	2	29	theme	simple	193:198	arg1	products					212:219	both simple and complex products	188:219	both simple and complex products	188:219	The initial characterization of both simple and complex products should be performed as soon as practical.
30737752	1	30	theme	major	111:115	arg1	importance					117:126	major importance	111:126	major importance	111:126	The product quality of recombinant proteins is of major importance for their intended purpose.
30737752	5	31	theme	glycan	717:722	arg1	analysis					724:731	glycan analysis	717:731	glycan analysis	717:731	Methods applied for the determination of posttranslational modifications such as glycan analysis are not described.
30737752	6	32	theme	Western	855:861	arg1	blot					863:866	Western blot	855:866	the integrity of proteins as well as Western blot	818:866	In this regard, chromatographic, high-resolution technologies for the integrity of proteins as well as Western blot with specific detection methods are introduced, and individual strengths and perceived limitations are highlighted.
30737752	3	33	theme	appropriate	307:317	arg1	selection					319:327	appropriate selection	307:327	appropriate selection of complementary methods	307:352	However, to comply with this high standard, appropriate selection of complementary methods is required.
30737752	6	34	dep	chromatographic	768:782	arg1	high-resolution					785:799	high-resolution	785:799	high-resolution	785:799	In this regard, chromatographic, high-resolution technologies for the integrity of proteins as well as Western blot with specific detection methods are introduced, and individual strengths and perceived limitations are highlighted.
30737752	4	35	theme	peptide	615:621	arg1	quality					584:590	the overall protein quality	564:590	the overall protein quality	564:590	Therefore, conventional and sophisticated techniques are available, providing diverse information about the product quality.In this chapter methods are presented, which enable the determination of the overall protein quality, their aggregation and peptide composition.
30737752	4	35	theme	peptide	615:621	arg1	composition					623:633	peptide composition	615:633	peptide composition	615:633	Therefore, conventional and sophisticated techniques are available, providing diverse information about the product quality.In this chapter methods are presented, which enable the determination of the overall protein quality, their aggregation and peptide composition.
30073041	0	0	theme	Methanolic	82:91	arg1	Extract					93:99	Methanolic Extract	82:99	Methanolic Extract of Euphorbia retusa	82:119	Chemical Composition and Antioxidant, Analgesic, and Anti-Inflammatory Effects of Methanolic Extract of Euphorbia retusa in Mice.
30073041	5	1	theme	antinociceptive	754:768	arg1	activity					770:777	The antinociceptive activity	750:777	The antinociceptive activity	750:777	The antinociceptive activity was evaluated using acetic acid and hot-plate models of pain in mice.
30073041	1	2	theme	alternative	148:158	arg1	source					160:165	an alternative source	145:165	an alternative source to manage different human disorders due to various metabolites	145:228	Plants provide an alternative source to manage different human disorders due to various metabolites.
30073041	7	3	theme	standard	1130:1137	arg1	paracetamol					1144:1154	the standard drug paracetamol	1126:1154	the standard drug paracetamol	1126:1154	Oral pretreatment with the methanolic extract of E. retusa (200 mg/kg) exhibited a significant inhibition of pain induced either by acetic acid or by the heating plate and in a manner comparable to the standard drug paracetamol.
30073041	0	4	from	Composition	9:19	arg1	Mice					124:127	Mice	124:127	Mice	124:127	Chemical Composition and Antioxidant, Analgesic, and Anti-Inflammatory Effects of Methanolic Extract of Euphorbia retusa in Mice.
30073041	7	5	theme	comparable	1112:1121	arg1	manner					1105:1110	a manner	1103:1110	a manner comparable to the standard drug paracetamol	1103:1154	Oral pretreatment with the methanolic extract of E. retusa (200 mg/kg) exhibited a significant inhibition of pain induced either by acetic acid or by the heating plate and in a manner comparable to the standard drug paracetamol.
30073041	9	6	theme	plant	1448:1452	arg1	potential					1430:1438	the interesting potential	1414:1438	the interesting potential of this plant	1414:1452	These results may confirm the interesting potential of this plant as a treatment of various inflammatory and pain diseases.
30073041	9	6	theme	plant	1448:1452	arg1	treatment					1459:1467	a treatment	1457:1467	a treatment of various inflammatory and pain diseases	1457:1509	These results may confirm the interesting potential of this plant as a treatment of various inflammatory and pain diseases.
30073041	8	7	theme	paw	1342:1344	arg1	tissues					1346:1352	liver and paw tissues	1332:1352	liver and paw tissues	1332:1352	E. retusa significantly reduced paw edema starting from the 3rd hour after carrageenan administration by increasing the activity of antioxidant enzymes (SOD, CAT, and GPx) in liver and paw tissues and decreasing the levels of MDA.
30073041	6	8	theme	paw	917:919	arg1	edema					921:925	carrageenan-induced paw edema	897:925	carrageenan-induced paw edema	897:925	The anti-inflammatory activity was evaluated by carrageenan-induced paw edema.
30073041	7	9	theme	retusa	980:985	arg1	extract					966:972	the methanolic extract	951:972	the methanolic extract of E. retusa (200 mg/kg)	951:997	Oral pretreatment with the methanolic extract of E. retusa (200 mg/kg) exhibited a significant inhibition of pain induced either by acetic acid or by the heating plate and in a manner comparable to the standard drug paracetamol.
30073041	2	10	theme	phytochemical	275:287	arg1	constituents					289:300	the phytochemical constituents	271:300	the phytochemical constituents of the methanolic extracts of Euphorbia retusa	271:347	The aim of this study is to investigate the phytochemical constituents of the methanolic extracts of Euphorbia retusa and to evaluate their antioxidant, anti-inflammatory, and analgesic activities.
30073041	4	11	theme	FRAP	737:740	arg1	assays					742:747	the FRAP assays	733:747	the FRAP assays	733:747	The methanolic extract shows significant antioxidant activities in vitro, in the DPPH and the FRAP assays.
30073041	0	12	theme	Extract	93:99	arg1	Composition					9:19	Chemical Composition	0:19	Chemical Composition	0:19	Chemical Composition and Antioxidant, Analgesic, and Anti-Inflammatory Effects of Methanolic Extract of Euphorbia retusa in Mice.
30073041	0	12	theme	Extract	93:99	arg1	Effects					71:77	Antioxidant, Analgesic, and Anti-Inflammatory Effects	25:77	Antioxidant, Analgesic, and Anti-Inflammatory Effects of Methanolic Extract of Euphorbia retusa in Mice	25:127	Chemical Composition and Antioxidant, Analgesic, and Anti-Inflammatory Effects of Methanolic Extract of Euphorbia retusa in Mice.
30073041	6	13	theme	carrageenan-induced	897:915	arg1	edema					921:925	carrageenan-induced paw edema	897:925	carrageenan-induced paw edema	897:925	The anti-inflammatory activity was evaluated by carrageenan-induced paw edema.
30073041	3	14	from	richness	522:529	arg1	compounds					580:588	active compounds	573:588	active compounds	573:588	The phytochemical results obtained by HPLC and by chemical assay reactions have revealed the richness of the methanolic extract of E. retusa in active compounds, in particular polyphenols, flavonoids, and tannins.
30073041	9	15	theme	inflammatory	1480:1491	arg1	potential					1430:1438	the interesting potential	1414:1438	the interesting potential of this plant	1414:1452	These results may confirm the interesting potential of this plant as a treatment of various inflammatory and pain diseases.
30073041	9	15	theme	inflammatory	1480:1491	arg1	treatment					1459:1467	a treatment	1457:1467	a treatment of various inflammatory and pain diseases	1457:1509	These results may confirm the interesting potential of this plant as a treatment of various inflammatory and pain diseases.
30073041	3	16	theme	assay	488:492	arg1	reactions					494:502	chemical assay reactions	479:502	chemical assay reactions	479:502	The phytochemical results obtained by HPLC and by chemical assay reactions have revealed the richness of the methanolic extract of E. retusa in active compounds, in particular polyphenols, flavonoids, and tannins.
30073041	0	17	theme	retusa	114:119	arg1	Extract					93:99	Methanolic Extract	82:99	Methanolic Extract of Euphorbia retusa	82:119	Chemical Composition and Antioxidant, Analgesic, and Anti-Inflammatory Effects of Methanolic Extract of Euphorbia retusa in Mice.
30073041	5	18	from	models	825:830	arg1	mice					843:846	mice	843:846	mice	843:846	The antinociceptive activity was evaluated using acetic acid and hot-plate models of pain in mice.
30073041	4	19	theme	significant	672:682	arg1	activities					696:705	significant antioxidant activities	672:705	significant antioxidant activities	672:705	The methanolic extract shows significant antioxidant activities in vitro, in the DPPH and the FRAP assays.
30073041	5	20	from	acid	806:809	arg1	mice					843:846	mice	843:846	mice	843:846	The antinociceptive activity was evaluated using acetic acid and hot-plate models of pain in mice.
30073041	3	21	theme	phytochemical	433:445	arg1	results					447:453	The phytochemical results	429:453	The phytochemical results obtained by HPLC and by chemical assay reactions	429:502	The phytochemical results obtained by HPLC and by chemical assay reactions have revealed the richness of the methanolic extract of E. retusa in active compounds, in particular polyphenols, flavonoids, and tannins.
30073041	8	22	theme	antioxidant	1289:1299	arg1	enzymes					1301:1307	antioxidant enzymes	1289:1307	antioxidant enzymes (SOD, CAT, and GPx)	1289:1327	E. retusa significantly reduced paw edema starting from the 3rd hour after carrageenan administration by increasing the activity of antioxidant enzymes (SOD, CAT, and GPx) in liver and paw tissues and decreasing the levels of MDA.
30073041	8	22	theme	antioxidant	1289:1299	arg1	CAT					1315:1317	CAT	1315:1317	CAT	1315:1317	E. retusa significantly reduced paw edema starting from the 3rd hour after carrageenan administration by increasing the activity of antioxidant enzymes (SOD, CAT, and GPx) in liver and paw tissues and decreasing the levels of MDA.
30073041	8	22	theme	antioxidant	1289:1299	arg1	GPx					1324:1326	GPx	1324:1326	GPx	1324:1326	E. retusa significantly reduced paw edema starting from the 3rd hour after carrageenan administration by increasing the activity of antioxidant enzymes (SOD, CAT, and GPx) in liver and paw tissues and decreasing the levels of MDA.
30073041	8	22	theme	antioxidant	1289:1299	arg1	SOD					1310:1312	SOD	1310:1312	SOD	1310:1312	E. retusa significantly reduced paw edema starting from the 3rd hour after carrageenan administration by increasing the activity of antioxidant enzymes (SOD, CAT, and GPx) in liver and paw tissues and decreasing the levels of MDA.
30073041	3	23	theme	methanolic	538:547	arg1	extract					549:555	the methanolic extract	534:555	the methanolic extract of E. retusa	534:568	The phytochemical results obtained by HPLC and by chemical assay reactions have revealed the richness of the methanolic extract of E. retusa in active compounds, in particular polyphenols, flavonoids, and tannins.
30073041	2	24	theme	anti-inflammatory	384:400	arg1	activities					417:426	their antioxidant, anti-inflammatory, and analgesic activities	365:426	their antioxidant, anti-inflammatory, and analgesic activities	365:426	The aim of this study is to investigate the phytochemical constituents of the methanolic extracts of Euphorbia retusa and to evaluate their antioxidant, anti-inflammatory, and analgesic activities.
30073041	7	25	theme	methanolic	955:964	arg1	extract					966:972	the methanolic extract	951:972	the methanolic extract of E. retusa (200 mg/kg)	951:997	Oral pretreatment with the methanolic extract of E. retusa (200 mg/kg) exhibited a significant inhibition of pain induced either by acetic acid or by the heating plate and in a manner comparable to the standard drug paracetamol.
30073041	2	26	theme	methanolic	309:318	arg1	extracts					320:327	the methanolic extracts	305:327	the methanolic extracts of Euphorbia retusa	305:347	The aim of this study is to investigate the phytochemical constituents of the methanolic extracts of Euphorbia retusa and to evaluate their antioxidant, anti-inflammatory, and analgesic activities.
30073041	9	27	dep	inflammatory	1480:1491	arg1	diseases					1502:1509	diseases	1502:1509	diseases	1502:1509	These results may confirm the interesting potential of this plant as a treatment of various inflammatory and pain diseases.
30073041	1	28	theme	different	177:185	arg1	disorders					193:201	different human disorders	177:201	different human disorders	177:201	Plants provide an alternative source to manage different human disorders due to various metabolites.
30073041	0	29	theme	Chemical	0:7	arg1	Composition					9:19	Chemical Composition	0:19	Chemical Composition	0:19	Chemical Composition and Antioxidant, Analgesic, and Anti-Inflammatory Effects of Methanolic Extract of Euphorbia retusa in Mice.
30073041	7	30	with	pretreatment	933:944	arg1	extract					966:972	the methanolic extract	951:972	the methanolic extract of E. retusa (200 mg/kg)	951:997	Oral pretreatment with the methanolic extract of E. retusa (200 mg/kg) exhibited a significant inhibition of pain induced either by acetic acid or by the heating plate and in a manner comparable to the standard drug paracetamol.
30073041	7	31	theme	heating	1082:1088	arg1	plate					1090:1094	the heating plate	1078:1094	the heating plate	1078:1094	Oral pretreatment with the methanolic extract of E. retusa (200 mg/kg) exhibited a significant inhibition of pain induced either by acetic acid or by the heating plate and in a manner comparable to the standard drug paracetamol.
30073041	3	32	theme	active	573:578	arg1	compounds					580:588	active compounds	573:588	active compounds	573:588	The phytochemical results obtained by HPLC and by chemical assay reactions have revealed the richness of the methanolic extract of E. retusa in active compounds, in particular polyphenols, flavonoids, and tannins.
30073041	2	33	theme	antioxidant	371:381	arg1	activities					417:426	their antioxidant, anti-inflammatory, and analgesic activities	365:426	their antioxidant, anti-inflammatory, and analgesic activities	365:426	The aim of this study is to investigate the phytochemical constituents of the methanolic extracts of Euphorbia retusa and to evaluate their antioxidant, anti-inflammatory, and analgesic activities.
30073041	0	34	theme	Antioxidant	25:35	arg1	Effects					71:77	Antioxidant, Analgesic, and Anti-Inflammatory Effects	25:77	Antioxidant, Analgesic, and Anti-Inflammatory Effects of Methanolic Extract of Euphorbia retusa in Mice	25:127	Chemical Composition and Antioxidant, Analgesic, and Anti-Inflammatory Effects of Methanolic Extract of Euphorbia retusa in Mice.
30073041	8	35	theme	enzymes	1301:1307	arg1	activity					1277:1284	the activity	1273:1284	the activity of antioxidant enzymes (SOD, CAT, and GPx) in liver and paw tissues	1273:1352	E. retusa significantly reduced paw edema starting from the 3rd hour after carrageenan administration by increasing the activity of antioxidant enzymes (SOD, CAT, and GPx) in liver and paw tissues and decreasing the levels of MDA.
30073041	9	36	theme	interesting	1418:1428	arg1	potential					1430:1438	the interesting potential	1414:1438	the interesting potential of this plant	1414:1452	These results may confirm the interesting potential of this plant as a treatment of various inflammatory and pain diseases.
30073041	9	36	theme	interesting	1418:1428	arg1	treatment					1459:1467	a treatment	1457:1467	a treatment of various inflammatory and pain diseases	1457:1509	These results may confirm the interesting potential of this plant as a treatment of various inflammatory and pain diseases.
30073041	8	37	theme	paw	1189:1191	arg1	edema					1193:1197	paw edema starting	1189:1206	paw edema starting	1189:1206	E. retusa significantly reduced paw edema starting from the 3rd hour after carrageenan administration by increasing the activity of antioxidant enzymes (SOD, CAT, and GPx) in liver and paw tissues and decreasing the levels of MDA.
30073041	3	38	theme	retusa	563:568	arg1	extract					549:555	the methanolic extract	534:555	the methanolic extract of E. retusa	534:568	The phytochemical results obtained by HPLC and by chemical assay reactions have revealed the richness of the methanolic extract of E. retusa in active compounds, in particular polyphenols, flavonoids, and tannins.
30073041	5	39	theme	pain	835:838	arg1	acid					806:809	acetic acid	799:809	acetic acid	799:809	The antinociceptive activity was evaluated using acetic acid and hot-plate models of pain in mice.
30073041	5	39	theme	pain	835:838	arg1	models					825:830	hot-plate models	815:830	hot-plate models of pain in mice	815:846	The antinociceptive activity was evaluated using acetic acid and hot-plate models of pain in mice.
30073041	0	40	theme	Analgesic	38:46	arg1	Effects					71:77	Antioxidant, Analgesic, and Anti-Inflammatory Effects	25:77	Antioxidant, Analgesic, and Anti-Inflammatory Effects of Methanolic Extract of Euphorbia retusa in Mice	25:127	Chemical Composition and Antioxidant, Analgesic, and Anti-Inflammatory Effects of Methanolic Extract of Euphorbia retusa in Mice.
30073041	7	41	theme	acetic	1060:1065	arg1	acid					1067:1070	acetic acid	1060:1070	acetic acid	1060:1070	Oral pretreatment with the methanolic extract of E. retusa (200 mg/kg) exhibited a significant inhibition of pain induced either by acetic acid or by the heating plate and in a manner comparable to the standard drug paracetamol.
30073041	6	42	theme	anti-inflammatory	853:869	arg1	activity					871:878	The anti-inflammatory activity	849:878	The anti-inflammatory activity	849:878	The anti-inflammatory activity was evaluated by carrageenan-induced paw edema.
30073041	1	43	theme	human	187:191	arg1	disorders					193:201	different human disorders	177:201	different human disorders	177:201	Plants provide an alternative source to manage different human disorders due to various metabolites.
30073041	2	44	theme	analgesic	407:415	arg1	activities					417:426	their antioxidant, anti-inflammatory, and analgesic activities	365:426	their antioxidant, anti-inflammatory, and analgesic activities	365:426	The aim of this study is to investigate the phytochemical constituents of the methanolic extracts of Euphorbia retusa and to evaluate their antioxidant, anti-inflammatory, and analgesic activities.
30073041	7	45	theme	drug	1139:1142	arg1	paracetamol					1144:1154	the standard drug paracetamol	1126:1154	the standard drug paracetamol	1126:1154	Oral pretreatment with the methanolic extract of E. retusa (200 mg/kg) exhibited a significant inhibition of pain induced either by acetic acid or by the heating plate and in a manner comparable to the standard drug paracetamol.
30073041	8	46	theme	starting	1199:1206	arg1	edema					1193:1197	paw edema starting	1189:1206	paw edema starting	1189:1206	E. retusa significantly reduced paw edema starting from the 3rd hour after carrageenan administration by increasing the activity of antioxidant enzymes (SOD, CAT, and GPx) in liver and paw tissues and decreasing the levels of MDA.
30073041	0	47	from	Effects	71:77	arg1	Mice					124:127	Mice	124:127	Mice	124:127	Chemical Composition and Antioxidant, Analgesic, and Anti-Inflammatory Effects of Methanolic Extract of Euphorbia retusa in Mice.
30073041	3	48	theme	particular	594:603	arg1	polyphenols					605:615	particular polyphenols	594:615	particular polyphenols	594:615	The phytochemical results obtained by HPLC and by chemical assay reactions have revealed the richness of the methanolic extract of E. retusa in active compounds, in particular polyphenols, flavonoids, and tannins.
30073041	1	49	theme	various	210:216	arg1	metabolites					218:228	various metabolites	210:228	various metabolites	210:228	Plants provide an alternative source to manage different human disorders due to various metabolites.
30073041	8	50	theme	3rd	1217:1219	arg1	hour					1221:1224	the 3rd hour	1213:1224	the 3rd hour after carrageenan administration	1213:1257	E. retusa significantly reduced paw edema starting from the 3rd hour after carrageenan administration by increasing the activity of antioxidant enzymes (SOD, CAT, and GPx) in liver and paw tissues and decreasing the levels of MDA.
30073041	8	51	theme	carrageenan	1232:1242	arg1	administration					1244:1257	carrageenan administration	1232:1257	carrageenan administration	1232:1257	E. retusa significantly reduced paw edema starting from the 3rd hour after carrageenan administration by increasing the activity of antioxidant enzymes (SOD, CAT, and GPx) in liver and paw tissues and decreasing the levels of MDA.
30073041	5	52	from	mice	843:846	arg1	acid					806:809	acetic acid	799:809	acetic acid	799:809	The antinociceptive activity was evaluated using acetic acid and hot-plate models of pain in mice.
30073041	5	52	from	mice	843:846	arg1	models					825:830	hot-plate models	815:830	hot-plate models of pain in mice	815:846	The antinociceptive activity was evaluated using acetic acid and hot-plate models of pain in mice.
30073041	2	53	theme	retusa	342:347	arg1	extracts					320:327	the methanolic extracts	305:327	the methanolic extracts of Euphorbia retusa	305:347	The aim of this study is to investigate the phytochemical constituents of the methanolic extracts of Euphorbia retusa and to evaluate their antioxidant, anti-inflammatory, and analgesic activities.
30073041	5	54	theme	acetic	799:804	arg1	acid					806:809	acetic acid	799:809	acetic acid	799:809	The antinociceptive activity was evaluated using acetic acid and hot-plate models of pain in mice.
30073041	9	55	theme	pain	1497:1500	arg1	potential					1430:1438	the interesting potential	1414:1438	the interesting potential of this plant	1414:1452	These results may confirm the interesting potential of this plant as a treatment of various inflammatory and pain diseases.
30073041	9	55	theme	pain	1497:1500	arg1	treatment					1459:1467	a treatment	1457:1467	a treatment of various inflammatory and pain diseases	1457:1509	These results may confirm the interesting potential of this plant as a treatment of various inflammatory and pain diseases.
30073041	5	56	theme	hot-plate	815:823	arg1	models					825:830	hot-plate models	815:830	hot-plate models of pain in mice	815:846	The antinociceptive activity was evaluated using acetic acid and hot-plate models of pain in mice.
30073041	7	57	theme	pain	1037:1040	arg1	inhibition					1023:1032	a significant inhibition	1009:1032	a significant inhibition of pain induced either by acetic acid or by the heating plate and in a manner comparable to the standard drug paracetamol	1009:1154	Oral pretreatment with the methanolic extract of E. retusa (200 mg/kg) exhibited a significant inhibition of pain induced either by acetic acid or by the heating plate and in a manner comparable to the standard drug paracetamol.
30073041	8	58	theme	MDA	1383:1385	arg1	levels					1373:1378	the levels	1369:1378	the levels of MDA	1369:1385	E. retusa significantly reduced paw edema starting from the 3rd hour after carrageenan administration by increasing the activity of antioxidant enzymes (SOD, CAT, and GPx) in liver and paw tissues and decreasing the levels of MDA.
30073041	8	59	theme	liver	1332:1336	arg1	tissues					1346:1352	liver and paw tissues	1332:1352	liver and paw tissues	1332:1352	E. retusa significantly reduced paw edema starting from the 3rd hour after carrageenan administration by increasing the activity of antioxidant enzymes (SOD, CAT, and GPx) in liver and paw tissues and decreasing the levels of MDA.
30073041	4	60	dep	DPPH	724:727	arg1	the					720:722	the	720:722	the	720:722	The methanolic extract shows significant antioxidant activities in vitro, in the DPPH and the FRAP assays.
30073041	3	61	theme	chemical	479:486	arg1	reactions					494:502	chemical assay reactions	479:502	chemical assay reactions	479:502	The phytochemical results obtained by HPLC and by chemical assay reactions have revealed the richness of the methanolic extract of E. retusa in active compounds, in particular polyphenols, flavonoids, and tannins.
30073041	7	62	theme	Oral	928:931	arg1	pretreatment					933:944	Oral pretreatment	928:944	Oral pretreatment with the methanolic extract of E. retusa (200 mg/kg)	928:997	Oral pretreatment with the methanolic extract of E. retusa (200 mg/kg) exhibited a significant inhibition of pain induced either by acetic acid or by the heating plate and in a manner comparable to the standard drug paracetamol.
30073041	5	63	from	pain	835:838	arg1	mice					843:846	mice	843:846	mice	843:846	The antinociceptive activity was evaluated using acetic acid and hot-plate models of pain in mice.
30073041	8	64	dep	enzymes	1301:1307	arg1	enzymes					1301:1307	antioxidant enzymes	1289:1307	antioxidant enzymes (SOD, CAT, and GPx)	1289:1327	E. retusa significantly reduced paw edema starting from the 3rd hour after carrageenan administration by increasing the activity of antioxidant enzymes (SOD, CAT, and GPx) in liver and paw tissues and decreasing the levels of MDA.
30073041	8	64	dep	enzymes	1301:1307	arg1	CAT					1315:1317	CAT	1315:1317	CAT	1315:1317	E. retusa significantly reduced paw edema starting from the 3rd hour after carrageenan administration by increasing the activity of antioxidant enzymes (SOD, CAT, and GPx) in liver and paw tissues and decreasing the levels of MDA.
30073041	8	64	dep	enzymes	1301:1307	arg1	GPx					1324:1326	GPx	1324:1326	GPx	1324:1326	E. retusa significantly reduced paw edema starting from the 3rd hour after carrageenan administration by increasing the activity of antioxidant enzymes (SOD, CAT, and GPx) in liver and paw tissues and decreasing the levels of MDA.
30073041	8	64	dep	enzymes	1301:1307	arg1	SOD					1310:1312	SOD	1310:1312	SOD	1310:1312	E. retusa significantly reduced paw edema starting from the 3rd hour after carrageenan administration by increasing the activity of antioxidant enzymes (SOD, CAT, and GPx) in liver and paw tissues and decreasing the levels of MDA.
30073041	2	65	theme	study	247:251	arg1	aim					235:237	The aim	231:237	The aim of this study	231:251	The aim of this study is to investigate the phytochemical constituents of the methanolic extracts of Euphorbia retusa and to evaluate their antioxidant, anti-inflammatory, and analgesic activities.
30073041	8	66	from	activity	1277:1284	arg1	tissues					1346:1352	liver and paw tissues	1332:1352	liver and paw tissues	1332:1352	E. retusa significantly reduced paw edema starting from the 3rd hour after carrageenan administration by increasing the activity of antioxidant enzymes (SOD, CAT, and GPx) in liver and paw tissues and decreasing the levels of MDA.
30073041	2	67	theme	extracts	320:327	arg1	constituents					289:300	the phytochemical constituents	271:300	the phytochemical constituents of the methanolic extracts of Euphorbia retusa	271:347	The aim of this study is to investigate the phytochemical constituents of the methanolic extracts of Euphorbia retusa and to evaluate their antioxidant, anti-inflammatory, and analgesic activities.
30073041	7	68	theme	significant	1011:1021	arg1	inhibition					1023:1032	a significant inhibition	1009:1032	a significant inhibition of pain induced either by acetic acid or by the heating plate and in a manner comparable to the standard drug paracetamol	1009:1154	Oral pretreatment with the methanolic extract of E. retusa (200 mg/kg) exhibited a significant inhibition of pain induced either by acetic acid or by the heating plate and in a manner comparable to the standard drug paracetamol.
30073041	4	69	theme	methanolic	647:656	arg1	extract					658:664	The methanolic extract	643:664	The methanolic extract	643:664	The methanolic extract shows significant antioxidant activities in vitro, in the DPPH and the FRAP assays.
30073041	0	70	theme	Anti-Inflammatory	53:69	arg1	Effects					71:77	Antioxidant, Analgesic, and Anti-Inflammatory Effects	25:77	Antioxidant, Analgesic, and Anti-Inflammatory Effects of Methanolic Extract of Euphorbia retusa in Mice	25:127	Chemical Composition and Antioxidant, Analgesic, and Anti-Inflammatory Effects of Methanolic Extract of Euphorbia retusa in Mice.
30073041	3	71	theme	extract	549:555	arg1	richness					522:529	the richness	518:529	the richness of the methanolic extract of E. retusa in active compounds	518:588	The phytochemical results obtained by HPLC and by chemical assay reactions have revealed the richness of the methanolic extract of E. retusa in active compounds, in particular polyphenols, flavonoids, and tannins.
30073041	4	72	theme	antioxidant	684:694	arg1	activities					696:705	significant antioxidant activities	672:705	significant antioxidant activities	672:705	The methanolic extract shows significant antioxidant activities in vitro, in the DPPH and the FRAP assays.
29999523	7	0	with	transport	1098:1106	arg1	matrix					1144:1149	the extracellular matrix	1126:1149	the extracellular matrix	1126:1149	Those proteins were functionally related to lytic granule composition and transport, interaction with the extracellular matrix, DNA transcription or repair, and proliferation.
29999523	9	1	theme	competent	1398:1406	arg1	cells					1408:1412	functionally competent cells	1385:1412	functionally competent cells sharing many features of bona fide CD56dim NK cells in healthy individuals, but with some distinct characteristics	1385:1527	In conclusion, CD56neg NK cells constitute functionally competent cells sharing many features of bona fide CD56dim NK cells in healthy individuals, but with some distinct characteristics.
29999523	6	2	with	cells	926:930	arg1	proteins					980:987	nine up-regulated and three down-regulated proteins	937:987	nine up-regulated and three down-regulated proteins in the CD56neg NK cell population	937:1021	In the present study, twelve proteins discriminated CD56neg NK cells from CD56dim NK cells with nine up-regulated and three down-regulated proteins in the CD56neg NK cell population.
29999523	4	3	theme	NK	574:575	arg1	compartment					582:592	the main NK cell compartment	565:592	the main NK cell compartment	565:592	Unbiased large-scale surface receptor profiling clustered CD56neg cells as part of the main NK cell compartment and indicated an overall CD56dim -like phenotype.
29999523	5	4	theme	proteome	650:657	arg1	analyses					659:666	Total proteome analyses	644:666	Total proteome analyses of CD56neg NK cells	644:686	Total proteome analyses of CD56neg NK cells further confirmed their similarity with CD56dim NK cells, and revealed a complete cytolytic inventory with high levels of perforin and granzyme H and M.
29999523	5	5	theme	complete	761:768	arg1	inventory					780:788	a complete cytolytic inventory	759:788	a complete cytolytic inventory with high levels of perforin and granzyme H and M	759:838	Total proteome analyses of CD56neg NK cells further confirmed their similarity with CD56dim NK cells, and revealed a complete cytolytic inventory with high levels of perforin and granzyme H and M.
29999523	7	6	theme	granule	1074:1080	arg1	composition					1082:1092	lytic granule composition	1068:1092	lytic granule composition	1068:1092	Those proteins were functionally related to lytic granule composition and transport, interaction with the extracellular matrix, DNA transcription or repair, and proliferation.
29999523	5	7	theme	high	795:798	arg1	levels					800:805	high levels	795:805	high levels of perforin and granzyme H and M	795:838	Total proteome analyses of CD56neg NK cells further confirmed their similarity with CD56dim NK cells, and revealed a complete cytolytic inventory with high levels of perforin and granzyme H and M.
29999523	8	8	theme	NK	1237:1238	arg1	cells					1240:1244	CD56neg NK cells	1229:1244	CD56neg NK cells	1229:1244	Corroborating these results, CD56neg NK cells showed modest cytotoxicity, degranulation, and IFN-ɣ secretion as compared to CD56dim NK cells.
29999523	9	9	theme	bona	1439:1442	arg1	cells					1460:1464	bona fide CD56dim NK cells	1439:1464	bona fide CD56dim NK cells	1439:1464	In conclusion, CD56neg NK cells constitute functionally competent cells sharing many features of bona fide CD56dim NK cells in healthy individuals, but with some distinct characteristics.
29999523	4	10	theme	overall	611:617	arg1	phenotype					633:641	an overall CD56dim -like phenotype	608:641	an overall CD56dim -like phenotype	608:641	Unbiased large-scale surface receptor profiling clustered CD56neg cells as part of the main NK cell compartment and indicated an overall CD56dim -like phenotype.
29999523	1	11	theme	chronic	176:182	arg1	infection					190:198	chronic HIV-1 infection	176:198	chronic HIV-1 infection	176:198	NK cells lacking CD56 (CD56neg ) were first identified in chronic HIV-1 infection.
29999523	6	12	theme	NK	923:924	arg1	cells					926:930	CD56dim NK cells	915:930	CD56dim NK cells with nine up-regulated and three down-regulated proteins in the CD56neg NK cell population	915:1021	In the present study, twelve proteins discriminated CD56neg NK cells from CD56dim NK cells with nine up-regulated and three down-regulated proteins in the CD56neg NK cell population.
29999523	4	13	theme	-like	627:631	arg1	phenotype					633:641	an overall CD56dim -like phenotype	608:641	an overall CD56dim -like phenotype	608:641	Unbiased large-scale surface receptor profiling clustered CD56neg cells as part of the main NK cell compartment and indicated an overall CD56dim -like phenotype.
29999523	5	14	theme	H	832:832	arg1	levels					800:805	high levels	795:805	high levels of perforin and granzyme H and M	795:838	Total proteome analyses of CD56neg NK cells further confirmed their similarity with CD56dim NK cells, and revealed a complete cytolytic inventory with high levels of perforin and granzyme H and M.
29999523	5	15	with	similarity	712:721	arg1	cells					739:743	CD56dim NK cells	728:743	CD56dim NK cells	728:743	Total proteome analyses of CD56neg NK cells further confirmed their similarity with CD56dim NK cells, and revealed a complete cytolytic inventory with high levels of perforin and granzyme H and M.
29999523	9	16	theme	healthy	1469:1475	arg1	individuals					1477:1487	healthy individuals	1469:1487	healthy individuals	1469:1487	In conclusion, CD56neg NK cells constitute functionally competent cells sharing many features of bona fide CD56dim NK cells in healthy individuals, but with some distinct characteristics.
29999523	6	17	theme	NK	901:902	arg1	cells					904:908	CD56neg NK cells	893:908	CD56neg NK cells from CD56dim NK cells with nine up-regulated and three down-regulated proteins in the CD56neg NK cell population	893:1021	In the present study, twelve proteins discriminated CD56neg NK cells from CD56dim NK cells with nine up-regulated and three down-regulated proteins in the CD56neg NK cell population.
29999523	7	18	with	proliferation	1185:1197	arg1	matrix					1144:1149	the extracellular matrix	1126:1149	the extracellular matrix	1126:1149	Those proteins were functionally related to lytic granule composition and transport, interaction with the extracellular matrix, DNA transcription or repair, and proliferation.
29999523	5	19	theme	NK	679:680	arg1	cells					682:686	CD56neg NK cells	671:686	CD56neg NK cells	671:686	Total proteome analyses of CD56neg NK cells further confirmed their similarity with CD56dim NK cells, and revealed a complete cytolytic inventory with high levels of perforin and granzyme H and M.
29999523	7	20	with	interaction	1109:1119	arg1	matrix					1144:1149	the extracellular matrix	1126:1149	the extracellular matrix	1126:1149	Those proteins were functionally related to lytic granule composition and transport, interaction with the extracellular matrix, DNA transcription or repair, and proliferation.
29999523	9	21	theme	cells	1460:1464	arg1	features					1427:1434	many features	1422:1434	many features of bona fide CD56dim NK cells	1422:1464	In conclusion, CD56neg NK cells constitute functionally competent cells sharing many features of bona fide CD56dim NK cells in healthy individuals, but with some distinct characteristics.
29999523	2	22	theme	NK	218:219	arg1	cells					221:225	CD56neg NK cells	210:225	CD56neg NK cells	210:225	However, CD56neg NK cells also exist in healthy individuals, albeit in significantly lower numbers.
29999523	5	23	theme	CD56dim	728:734	arg1	cells					739:743	CD56dim NK cells	728:743	CD56dim NK cells	728:743	Total proteome analyses of CD56neg NK cells further confirmed their similarity with CD56dim NK cells, and revealed a complete cytolytic inventory with high levels of perforin and granzyme H and M.
29999523	9	24	theme	distinct	1504:1511	arg1	characteristics					1513:1527	some distinct characteristics	1499:1527	some distinct characteristics	1499:1527	In conclusion, CD56neg NK cells constitute functionally competent cells sharing many features of bona fide CD56dim NK cells in healthy individuals, but with some distinct characteristics.
29999523	4	25	theme	Unbiased	482:489	arg1	profiling					520:528	Unbiased large-scale surface receptor profiling	482:528	Unbiased large-scale surface receptor profiling	482:528	Unbiased large-scale surface receptor profiling clustered CD56neg cells as part of the main NK cell compartment and indicated an overall CD56dim -like phenotype.
29999523	8	26	theme	CD56dim	1324:1330	arg1	cells					1335:1339	CD56dim NK cells	1324:1339	CD56dim NK cells	1324:1339	Corroborating these results, CD56neg NK cells showed modest cytotoxicity, degranulation, and IFN-ɣ secretion as compared to CD56dim NK cells.
29999523	0	27	theme	similar	89:95	arg1	phenotype					66:74	a homogeneous phenotype	52:74	a homogeneous phenotype surprisingly similar to CD56dim NK cells	52:115	Proteome analysis of human CD56neg NK cells reveals a homogeneous phenotype surprisingly similar to CD56dim NK cells.
29999523	6	28	theme	up-regulated	942:953	arg1	proteins					980:987	nine up-regulated and three down-regulated proteins	937:987	nine up-regulated and three down-regulated proteins in the CD56neg NK cell population	937:1021	In the present study, twelve proteins discriminated CD56neg NK cells from CD56dim NK cells with nine up-regulated and three down-regulated proteins in the CD56neg NK cell population.
29999523	3	29	theme	healthy	404:410	arg1	donors					412:417	healthy donors	404:417	healthy donors	404:417	Here, we provide an extensive proteomic characterisation of human CD56neg peripheral blood NK cells of healthy donors and compare them to their CD56dim and CD56bright counterparts.
29999523	5	30	theme	NK	736:737	arg1	cells					739:743	CD56dim NK cells	728:743	CD56dim NK cells	728:743	Total proteome analyses of CD56neg NK cells further confirmed their similarity with CD56dim NK cells, and revealed a complete cytolytic inventory with high levels of perforin and granzyme H and M.
29999523	3	31	theme	cells	395:399	arg1	characterisation					341:356	an extensive proteomic characterisation	318:356	an extensive proteomic characterisation of human CD56neg peripheral blood NK cells of healthy donors	318:417	Here, we provide an extensive proteomic characterisation of human CD56neg peripheral blood NK cells of healthy donors and compare them to their CD56dim and CD56bright counterparts.
29999523	0	32	theme	Proteome	0:7	arg1	analysis					9:16	Proteome analysis	0:16	Proteome analysis of human CD56neg NK cells	0:42	Proteome analysis of human CD56neg NK cells reveals a homogeneous phenotype surprisingly similar to CD56dim NK cells.
29999523	3	33	theme	proteomic	331:339	arg1	characterisation					341:356	an extensive proteomic characterisation	318:356	an extensive proteomic characterisation of human CD56neg peripheral blood NK cells of healthy donors	318:417	Here, we provide an extensive proteomic characterisation of human CD56neg peripheral blood NK cells of healthy donors and compare them to their CD56dim and CD56bright counterparts.
29999523	4	34	theme	CD56neg	540:546	arg1	cells					548:552	CD56neg cells	540:552	CD56neg cells	540:552	Unbiased large-scale surface receptor profiling clustered CD56neg cells as part of the main NK cell compartment and indicated an overall CD56dim -like phenotype.
29999523	6	35	from	cells	926:930	arg1	cells					904:908	CD56neg NK cells	893:908	CD56neg NK cells from CD56dim NK cells with nine up-regulated and three down-regulated proteins in the CD56neg NK cell population	893:1021	In the present study, twelve proteins discriminated CD56neg NK cells from CD56dim NK cells with nine up-regulated and three down-regulated proteins in the CD56neg NK cell population.
29999523	3	36	theme	blood	386:390	arg1	cells					395:399	human CD56neg peripheral blood NK cells	361:399	human CD56neg peripheral blood NK cells of healthy donors	361:417	Here, we provide an extensive proteomic characterisation of human CD56neg peripheral blood NK cells of healthy donors and compare them to their CD56dim and CD56bright counterparts.
29999523	7	37	theme	transcription	1156:1168	arg1	repair					1173:1178	DNA transcription or repair	1152:1178	DNA transcription or repair	1152:1178	Those proteins were functionally related to lytic granule composition and transport, interaction with the extracellular matrix, DNA transcription or repair, and proliferation.
29999523	7	38	theme	extracellular	1130:1142	arg1	matrix					1144:1149	the extracellular matrix	1126:1149	the extracellular matrix	1126:1149	Those proteins were functionally related to lytic granule composition and transport, interaction with the extracellular matrix, DNA transcription or repair, and proliferation.
29999523	0	39	theme	CD56neg	27:33	arg1	cells					38:42	human CD56neg NK cells	21:42	human CD56neg NK cells	21:42	Proteome analysis of human CD56neg NK cells reveals a homogeneous phenotype surprisingly similar to CD56dim NK cells.
29999523	6	40	theme	NK	1004:1005	arg1	population					1012:1021	the CD56neg NK cell population	992:1021	the CD56neg NK cell population	992:1021	In the present study, twelve proteins discriminated CD56neg NK cells from CD56dim NK cells with nine up-regulated and three down-regulated proteins in the CD56neg NK cell population.
29999523	3	41	theme	CD56neg	367:373	arg1	cells					395:399	human CD56neg peripheral blood NK cells	361:399	human CD56neg peripheral blood NK cells of healthy donors	361:417	Here, we provide an extensive proteomic characterisation of human CD56neg peripheral blood NK cells of healthy donors and compare them to their CD56dim and CD56bright counterparts.
29999523	4	42	theme	surface	503:509	arg1	profiling					520:528	Unbiased large-scale surface receptor profiling	482:528	Unbiased large-scale surface receptor profiling	482:528	Unbiased large-scale surface receptor profiling clustered CD56neg cells as part of the main NK cell compartment and indicated an overall CD56dim -like phenotype.
29999523	0	43	theme	cells	38:42	arg1	analysis					9:16	Proteome analysis	0:16	Proteome analysis of human CD56neg NK cells	0:42	Proteome analysis of human CD56neg NK cells reveals a homogeneous phenotype surprisingly similar to CD56dim NK cells.
29999523	9	44	theme	CD56neg	1357:1363	arg1	cells					1368:1372	CD56neg NK cells	1357:1372	CD56neg NK cells	1357:1372	In conclusion, CD56neg NK cells constitute functionally competent cells sharing many features of bona fide CD56dim NK cells in healthy individuals, but with some distinct characteristics.
29999523	4	45	theme	cell	577:580	arg1	compartment					582:592	the main NK cell compartment	565:592	the main NK cell compartment	565:592	Unbiased large-scale surface receptor profiling clustered CD56neg cells as part of the main NK cell compartment and indicated an overall CD56dim -like phenotype.
29999523	7	46	theme	or	1170:1171	arg1	repair					1173:1178	DNA transcription or repair	1152:1178	DNA transcription or repair	1152:1178	Those proteins were functionally related to lytic granule composition and transport, interaction with the extracellular matrix, DNA transcription or repair, and proliferation.
29999523	5	47	theme	Total	644:648	arg1	analyses					659:666	Total proteome analyses	644:666	Total proteome analyses of CD56neg NK cells	644:686	Total proteome analyses of CD56neg NK cells further confirmed their similarity with CD56dim NK cells, and revealed a complete cytolytic inventory with high levels of perforin and granzyme H and M.
29999523	5	48	with	inventory	780:788	arg1	levels					800:805	high levels	795:805	high levels of perforin and granzyme H and M	795:838	Total proteome analyses of CD56neg NK cells further confirmed their similarity with CD56dim NK cells, and revealed a complete cytolytic inventory with high levels of perforin and granzyme H and M.
29999523	4	49	theme	main	569:572	arg1	compartment					582:592	the main NK cell compartment	565:592	the main NK cell compartment	565:592	Unbiased large-scale surface receptor profiling clustered CD56neg cells as part of the main NK cell compartment and indicated an overall CD56dim -like phenotype.
29999523	7	50	theme	lytic	1068:1072	arg1	composition					1082:1092	lytic granule composition	1068:1092	lytic granule composition	1068:1092	Those proteins were functionally related to lytic granule composition and transport, interaction with the extracellular matrix, DNA transcription or repair, and proliferation.
29999523	2	51	theme	healthy	241:247	arg1	individuals					249:259	healthy individuals	241:259	healthy individuals	241:259	However, CD56neg NK cells also exist in healthy individuals, albeit in significantly lower numbers.
29999523	9	52	theme	many	1422:1425	arg1	features					1427:1434	many features	1422:1434	many features of bona fide CD56dim NK cells	1422:1464	In conclusion, CD56neg NK cells constitute functionally competent cells sharing many features of bona fide CD56dim NK cells in healthy individuals, but with some distinct characteristics.
29999523	6	53	theme	present	848:854	arg1	study					856:860	the present study	844:860	the present study	844:860	In the present study, twelve proteins discriminated CD56neg NK cells from CD56dim NK cells with nine up-regulated and three down-regulated proteins in the CD56neg NK cell population.
29999523	2	54	theme	lower	286:290	arg1	numbers					292:298	significantly lower numbers	272:298	significantly lower numbers	272:298	However, CD56neg NK cells also exist in healthy individuals, albeit in significantly lower numbers.
29999523	6	55	dep	down-regulated	965:978	arg1	three					959:963	three	959:963	three	959:963	In the present study, twelve proteins discriminated CD56neg NK cells from CD56dim NK cells with nine up-regulated and three down-regulated proteins in the CD56neg NK cell population.
29999523	5	56	dep	complete	761:768	arg1	cytolytic					770:778	cytolytic	770:778	cytolytic	770:778	Total proteome analyses of CD56neg NK cells further confirmed their similarity with CD56dim NK cells, and revealed a complete cytolytic inventory with high levels of perforin and granzyme H and M.
29999523	8	57	theme	CD56neg	1229:1235	arg1	cells					1240:1244	CD56neg NK cells	1229:1244	CD56neg NK cells	1229:1244	Corroborating these results, CD56neg NK cells showed modest cytotoxicity, degranulation, and IFN-ɣ secretion as compared to CD56dim NK cells.
29999523	5	58	theme	cells	682:686	arg1	analyses					659:666	Total proteome analyses	644:666	Total proteome analyses of CD56neg NK cells	644:686	Total proteome analyses of CD56neg NK cells further confirmed their similarity with CD56dim NK cells, and revealed a complete cytolytic inventory with high levels of perforin and granzyme H and M.
29999523	5	59	theme	perforin	810:817	arg1	levels					800:805	high levels	795:805	high levels of perforin and granzyme H and M	795:838	Total proteome analyses of CD56neg NK cells further confirmed their similarity with CD56dim NK cells, and revealed a complete cytolytic inventory with high levels of perforin and granzyme H and M.
29999523	2	60	theme	CD56neg	210:216	arg1	cells					221:225	CD56neg NK cells	210:225	CD56neg NK cells	210:225	However, CD56neg NK cells also exist in healthy individuals, albeit in significantly lower numbers.
29999523	5	61	theme	granzyme	823:830	arg1	H					832:832	granzyme H	823:832	granzyme H	823:832	Total proteome analyses of CD56neg NK cells further confirmed their similarity with CD56dim NK cells, and revealed a complete cytolytic inventory with high levels of perforin and granzyme H and M.
29999523	6	62	theme	CD56dim	915:921	arg1	cells					926:930	CD56dim NK cells	915:930	CD56dim NK cells with nine up-regulated and three down-regulated proteins in the CD56neg NK cell population	915:1021	In the present study, twelve proteins discriminated CD56neg NK cells from CD56dim NK cells with nine up-regulated and three down-regulated proteins in the CD56neg NK cell population.
29999523	7	63	with	repair	1173:1178	arg1	matrix					1144:1149	the extracellular matrix	1126:1149	the extracellular matrix	1126:1149	Those proteins were functionally related to lytic granule composition and transport, interaction with the extracellular matrix, DNA transcription or repair, and proliferation.
29999523	8	64	theme	modest	1253:1258	arg1	cytotoxicity					1260:1271	modest cytotoxicity	1253:1271	modest cytotoxicity	1253:1271	Corroborating these results, CD56neg NK cells showed modest cytotoxicity, degranulation, and IFN-ɣ secretion as compared to CD56dim NK cells.
29999523	5	65	theme	CD56neg	671:677	arg1	cells					682:686	CD56neg NK cells	671:686	CD56neg NK cells	671:686	Total proteome analyses of CD56neg NK cells further confirmed their similarity with CD56dim NK cells, and revealed a complete cytolytic inventory with high levels of perforin and granzyme H and M.
29999523	1	66	theme	NK	118:119	arg1	cells					121:125	NK cells	118:125	NK cells lacking CD56 (CD56neg )	118:149	NK cells lacking CD56 (CD56neg ) were first identified in chronic HIV-1 infection.
29999523	6	67	theme	CD56neg	996:1002	arg1	population					1012:1021	the CD56neg NK cell population	992:1021	the CD56neg NK cell population	992:1021	In the present study, twelve proteins discriminated CD56neg NK cells from CD56dim NK cells with nine up-regulated and three down-regulated proteins in the CD56neg NK cell population.
29999523	0	68	theme	homogeneous	54:64	arg1	phenotype					66:74	a homogeneous phenotype	52:74	a homogeneous phenotype surprisingly similar to CD56dim NK cells	52:115	Proteome analysis of human CD56neg NK cells reveals a homogeneous phenotype surprisingly similar to CD56dim NK cells.
29999523	8	69	theme	IFN-ɣ	1293:1297	arg1	secretion					1299:1307	IFN-ɣ secretion	1293:1307	IFN-ɣ secretion	1293:1307	Corroborating these results, CD56neg NK cells showed modest cytotoxicity, degranulation, and IFN-ɣ secretion as compared to CD56dim NK cells.
29999523	6	70	theme	down-regulated	965:978	arg1	proteins					980:987	nine up-regulated and three down-regulated proteins	937:987	nine up-regulated and three down-regulated proteins in the CD56neg NK cell population	937:1021	In the present study, twelve proteins discriminated CD56neg NK cells from CD56dim NK cells with nine up-regulated and three down-regulated proteins in the CD56neg NK cell population.
29999523	4	71	theme	large-scale	491:501	arg1	profiling					520:528	Unbiased large-scale surface receptor profiling	482:528	Unbiased large-scale surface receptor profiling	482:528	Unbiased large-scale surface receptor profiling clustered CD56neg cells as part of the main NK cell compartment and indicated an overall CD56dim -like phenotype.
29999523	6	72	from	proteins	980:987	arg1	population					1012:1021	the CD56neg NK cell population	992:1021	the CD56neg NK cell population	992:1021	In the present study, twelve proteins discriminated CD56neg NK cells from CD56dim NK cells with nine up-regulated and three down-regulated proteins in the CD56neg NK cell population.
29999523	3	73	theme	extensive	321:329	arg1	characterisation					341:356	an extensive proteomic characterisation	318:356	an extensive proteomic characterisation of human CD56neg peripheral blood NK cells of healthy donors	318:417	Here, we provide an extensive proteomic characterisation of human CD56neg peripheral blood NK cells of healthy donors and compare them to their CD56dim and CD56bright counterparts.
29999523	0	74	theme	NK	108:109	arg1	cells					111:115	CD56dim NK cells	100:115	CD56dim NK cells	100:115	Proteome analysis of human CD56neg NK cells reveals a homogeneous phenotype surprisingly similar to CD56dim NK cells.
29999523	9	75	theme	CD56dim	1449:1455	arg1	cells					1460:1464	bona fide CD56dim NK cells	1439:1464	bona fide CD56dim NK cells	1439:1464	In conclusion, CD56neg NK cells constitute functionally competent cells sharing many features of bona fide CD56dim NK cells in healthy individuals, but with some distinct characteristics.
29999523	8	76	theme	NK	1332:1333	arg1	cells					1335:1339	CD56dim NK cells	1324:1339	CD56dim NK cells	1324:1339	Corroborating these results, CD56neg NK cells showed modest cytotoxicity, degranulation, and IFN-ɣ secretion as compared to CD56dim NK cells.
29999523	3	77	theme	donors	412:417	arg1	cells					395:399	human CD56neg peripheral blood NK cells	361:399	human CD56neg peripheral blood NK cells of healthy donors	361:417	Here, we provide an extensive proteomic characterisation of human CD56neg peripheral blood NK cells of healthy donors and compare them to their CD56dim and CD56bright counterparts.
29999523	7	78	theme	DNA	1152:1154	arg1	repair					1173:1178	DNA transcription or repair	1152:1178	DNA transcription or repair	1152:1178	Those proteins were functionally related to lytic granule composition and transport, interaction with the extracellular matrix, DNA transcription or repair, and proliferation.
29999523	3	79	theme	human	361:365	arg1	cells					395:399	human CD56neg peripheral blood NK cells	361:399	human CD56neg peripheral blood NK cells of healthy donors	361:417	Here, we provide an extensive proteomic characterisation of human CD56neg peripheral blood NK cells of healthy donors and compare them to their CD56dim and CD56bright counterparts.
29999523	0	80	theme	human	21:25	arg1	cells					38:42	human CD56neg NK cells	21:42	human CD56neg NK cells	21:42	Proteome analysis of human CD56neg NK cells reveals a homogeneous phenotype surprisingly similar to CD56dim NK cells.
29999523	0	81	theme	NK	35:36	arg1	cells					38:42	human CD56neg NK cells	21:42	human CD56neg NK cells	21:42	Proteome analysis of human CD56neg NK cells reveals a homogeneous phenotype surprisingly similar to CD56dim NK cells.
29999523	1	82	theme	HIV-1	184:188	arg1	infection					190:198	chronic HIV-1 infection	176:198	chronic HIV-1 infection	176:198	NK cells lacking CD56 (CD56neg ) were first identified in chronic HIV-1 infection.
29999523	6	83	theme	cell	1007:1010	arg1	population					1012:1021	the CD56neg NK cell population	992:1021	the CD56neg NK cell population	992:1021	In the present study, twelve proteins discriminated CD56neg NK cells from CD56dim NK cells with nine up-regulated and three down-regulated proteins in the CD56neg NK cell population.
29999523	3	84	theme	NK	392:393	arg1	cells					395:399	human CD56neg peripheral blood NK cells	361:399	human CD56neg peripheral blood NK cells of healthy donors	361:417	Here, we provide an extensive proteomic characterisation of human CD56neg peripheral blood NK cells of healthy donors and compare them to their CD56dim and CD56bright counterparts.
29999523	4	85	theme	receptor	511:518	arg1	profiling					520:528	Unbiased large-scale surface receptor profiling	482:528	Unbiased large-scale surface receptor profiling	482:528	Unbiased large-scale surface receptor profiling clustered CD56neg cells as part of the main NK cell compartment and indicated an overall CD56dim -like phenotype.
29999523	9	86	dep	bona	1439:1442	arg1	fide					1444:1447	fide	1444:1447	fide	1444:1447	In conclusion, CD56neg NK cells constitute functionally competent cells sharing many features of bona fide CD56dim NK cells in healthy individuals, but with some distinct characteristics.
29999523	0	87	theme	CD56dim	100:106	arg1	cells					111:115	CD56dim NK cells	100:115	CD56dim NK cells	100:115	Proteome analysis of human CD56neg NK cells reveals a homogeneous phenotype surprisingly similar to CD56dim NK cells.
29999523	9	88	theme	NK	1457:1458	arg1	cells					1460:1464	bona fide CD56dim NK cells	1439:1464	bona fide CD56dim NK cells	1439:1464	In conclusion, CD56neg NK cells constitute functionally competent cells sharing many features of bona fide CD56dim NK cells in healthy individuals, but with some distinct characteristics.
29999523	3	89	theme	peripheral	375:384	arg1	cells					395:399	human CD56neg peripheral blood NK cells	361:399	human CD56neg peripheral blood NK cells of healthy donors	361:417	Here, we provide an extensive proteomic characterisation of human CD56neg peripheral blood NK cells of healthy donors and compare them to their CD56dim and CD56bright counterparts.
29999523	6	90	theme	CD56neg	893:899	arg1	cells					904:908	CD56neg NK cells	893:908	CD56neg NK cells from CD56dim NK cells with nine up-regulated and three down-regulated proteins in the CD56neg NK cell population	893:1021	In the present study, twelve proteins discriminated CD56neg NK cells from CD56dim NK cells with nine up-regulated and three down-regulated proteins in the CD56neg NK cell population.
29999523	3	91	theme	CD56bright	457:466	arg1	counterparts					468:479	their CD56dim and CD56bright counterparts	439:479	their CD56dim and CD56bright counterparts	439:479	Here, we provide an extensive proteomic characterisation of human CD56neg peripheral blood NK cells of healthy donors and compare them to their CD56dim and CD56bright counterparts.
29999523	4	92	theme	compartment	582:592	arg1	part					557:560	part	557:560	part of the main NK cell compartment	557:592	Unbiased large-scale surface receptor profiling clustered CD56neg cells as part of the main NK cell compartment and indicated an overall CD56dim -like phenotype.
29999523	3	93	theme	CD56dim	445:451	arg1	counterparts					468:479	their CD56dim and CD56bright counterparts	439:479	their CD56dim and CD56bright counterparts	439:479	Here, we provide an extensive proteomic characterisation of human CD56neg peripheral blood NK cells of healthy donors and compare them to their CD56dim and CD56bright counterparts.
29999523	9	94	theme	NK	1365:1366	arg1	cells					1368:1372	CD56neg NK cells	1357:1372	CD56neg NK cells	1357:1372	In conclusion, CD56neg NK cells constitute functionally competent cells sharing many features of bona fide CD56dim NK cells in healthy individuals, but with some distinct characteristics.
29999523	4	95	theme	CD56dim	619:625	arg1	phenotype					633:641	an overall CD56dim -like phenotype	608:641	an overall CD56dim -like phenotype	608:641	Unbiased large-scale surface receptor profiling clustered CD56neg cells as part of the main NK cell compartment and indicated an overall CD56dim -like phenotype.
29999523	7	96	with	composition	1082:1092	arg1	matrix					1144:1149	the extracellular matrix	1126:1149	the extracellular matrix	1126:1149	Those proteins were functionally related to lytic granule composition and transport, interaction with the extracellular matrix, DNA transcription or repair, and proliferation.
29300151	8	0	theme	nucleotide	1050:1059	arg1	identity					1061:1068	average nucleotide identity	1042:1068	The average nucleotide identity values between strain 7578-1T and its most closely related species	1038:1135	The average nucleotide identity values between strain 7578-1T and its most closely related species were 67.8-82.4 % by OrthoANIu analysis.
29300151	7	1	theme	unidentified	1018:1029	arg1	lipid					1031:1035	one unidentified lipid	1014:1035	one unidentified lipid	1014:1035	The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, one unidentified phospholipid and one unidentified lipid.
29300151	9	2	theme	type	1238:1241	arg1	strains					1243:1249	the type strains	1234:1249	the type strains of closely related species	1234:1276	The DNA-DNA relatedness value between strain 7578-1T and the type strains of closely related species were 17-39 %, again indicating that strain 7578-1T represented a novel species in the genus Bacillus.
29300151	5	3	theme	meso-diaminopimelic	746:764	arg1	acid					766:769	meso-diaminopimelic acid	746:769	meso-diaminopimelic acid	746:769	The strain had a cell-wall type A1γ peptidoglycan with meso-diaminopimelic acid as the diagnostic diamino acid.
29300151	9	4	theme	strain	1314:1319	arg1	7578-1T					1321:1327	strain 7578-1T	1314:1327	strain 7578-1T	1314:1327	The DNA-DNA relatedness value between strain 7578-1T and the type strains of closely related species were 17-39 %, again indicating that strain 7578-1T represented a novel species in the genus Bacillus.
29300151	8	5	theme	average	1042:1048	arg1	identity					1061:1068	average nucleotide identity	1042:1068	The average nucleotide identity values between strain 7578-1T and its most closely related species	1038:1135	The average nucleotide identity values between strain 7578-1T and its most closely related species were 67.8-82.4 % by OrthoANIu analysis.
29300151	2	6	dep	aerobic	62:68	arg1	rod-shaped					105:114	rod-shaped	105:114	rod-shaped	105:114	A novel aerobic, Gram-stain-positive, sporogenous, rod-shaped bacterial strain, 7578-1T, was isolated from ripened Pu'er tea.
29300151	2	6	dep	aerobic	62:68	arg1	sporogenous					92:102	sporogenous	92:102	sporogenous	92:102	A novel aerobic, Gram-stain-positive, sporogenous, rod-shaped bacterial strain, 7578-1T, was isolated from ripened Pu'er tea.
29300151	2	6	dep	aerobic	62:68	arg1	Gram-stain-positive					71:89	Gram-stain-positive	71:89	Gram-stain-positive	71:89	A novel aerobic, Gram-stain-positive, sporogenous, rod-shaped bacterial strain, 7578-1T, was isolated from ripened Pu'er tea.
29300151	11	7	dep	name	1590:1593	arg1	sp					1614:1615	Bacillus camelliae sp	1595:1615	the name Bacillus camelliae sp	1586:1615	On the basis of the presented polyphasic evidence, strain 7578-1T is considered to represent a novel species of the genus Bacillus, for which we propose the name Bacillus camelliae sp.
29300151	9	8	theme	strain	1215:1220	arg1	7578-1T					1222:1228	strain 7578-1T	1215:1228	strain 7578-1T	1215:1228	The DNA-DNA relatedness value between strain 7578-1T and the type strains of closely related species were 17-39 %, again indicating that strain 7578-1T represented a novel species in the genus Bacillus.
29300151	5	9	with	peptidoglycan	727:739	arg1	acid					766:769	meso-diaminopimelic acid	746:769	meso-diaminopimelic acid	746:769	The strain had a cell-wall type A1γ peptidoglycan with meso-diaminopimelic acid as the diagnostic diamino acid.
29300151	2	10	theme	er	172:173	arg1	tea					175:177	ripened Pu'er tea	161:177	ripened Pu'er tea	161:177	A novel aerobic, Gram-stain-positive, sporogenous, rod-shaped bacterial strain, 7578-1T, was isolated from ripened Pu'er tea.
29300151	4	11	dep	iso-C15 	576:583	arg1	 0					622:623	 0	622:623	 0	622:623	The fatty acid profile containing the major fatty acids, iso-C15 : 0, anteiso-C15 : 0 and anteiso-C17 : 0 supported the allocation of strain 7578-1T to the genus Bacillus.
29300151	4	11	dep	iso-C15 	576:583	arg1	anteiso-C17 					609:620	anteiso-C17 	609:620	anteiso-C17 	609:620	The fatty acid profile containing the major fatty acids, iso-C15 : 0, anteiso-C15 : 0 and anteiso-C17 : 0 supported the allocation of strain 7578-1T to the genus Bacillus.
29300151	4	11	dep	iso-C15 	576:583	arg1	 0					602:603	 0	602:603	 0	602:603	The fatty acid profile containing the major fatty acids, iso-C15 : 0, anteiso-C15 : 0 and anteiso-C17 : 0 supported the allocation of strain 7578-1T to the genus Bacillus.
29300151	4	11	dep	iso-C15 	576:583	arg1	 0					585:586	 0	585:586	 0	585:586	The fatty acid profile containing the major fatty acids, iso-C15 : 0, anteiso-C15 : 0 and anteiso-C17 : 0 supported the allocation of strain 7578-1T to the genus Bacillus.
29300151	9	12	from	species	1349:1355	arg1	Bacillus					1370:1377	genus Bacillus	1364:1377	the genus Bacillus	1360:1377	The DNA-DNA relatedness value between strain 7578-1T and the type strains of closely related species were 17-39 %, again indicating that strain 7578-1T represented a novel species in the genus Bacillus.
29300151	8	13	theme	identity	1061:1068	arg1	values					1070:1075	The average nucleotide identity values	1038:1075	The average nucleotide identity values between strain 7578-1T and its most closely related species	1038:1135	The average nucleotide identity values between strain 7578-1T and its most closely related species were 67.8-82.4 % by OrthoANIu analysis.
29300151	8	13	theme	identity	1061:1068	arg1	%					1152:1152	67.8-82.4 %	1142:1152	67.8-82.4 %	1142:1152	The average nucleotide identity values between strain 7578-1T and its most closely related species were 67.8-82.4 % by OrthoANIu analysis.
29300151	11	14	theme	Bacillus	1555:1562	arg1	species					1534:1540	a novel species	1526:1540	a novel species	1526:1540	On the basis of the presented polyphasic evidence, strain 7578-1T is considered to represent a novel species of the genus Bacillus, for which we propose the name Bacillus camelliae sp.
29300151	11	15	theme	Bacillus	1595:1602	arg1	sp					1614:1615	Bacillus camelliae sp	1595:1615	the name Bacillus camelliae sp	1586:1615	On the basis of the presented polyphasic evidence, strain 7578-1T is considered to represent a novel species of the genus Bacillus, for which we propose the name Bacillus camelliae sp.
29300151	13	16	theme	=CGMCC	1651:1656	arg1	7578-1T					1642:1648	7578-1T	1642:1648	7578-1T (=CGMCC 1.15374T=KCTC 33845T)	1642:1678	The type strain is 7578-1T (=CGMCC 1.15374T=KCTC 33845T).
29300151	13	16	theme	=CGMCC	1651:1656	arg1	33845T					1672:1677	=CGMCC 1.15374T=KCTC 33845T	1651:1677	=CGMCC 1.15374T=KCTC 33845T	1651:1677	The type strain is 7578-1T (=CGMCC 1.15374T=KCTC 33845T).
29300151	11	17	theme	presented	1453:1461	arg1	evidence					1474:1481	the presented polyphasic evidence	1449:1481	the presented polyphasic evidence	1449:1481	On the basis of the presented polyphasic evidence, strain 7578-1T is considered to represent a novel species of the genus Bacillus, for which we propose the name Bacillus camelliae sp.
29300151	5	18	theme	diamino	789:795	arg1	acid					797:800	the diagnostic diamino acid	774:800	the diagnostic diamino acid	774:800	The strain had a cell-wall type A1γ peptidoglycan with meso-diaminopimelic acid as the diagnostic diamino acid.
29300151	5	18	theme	diamino	789:795	arg1	peptidoglycan					727:739	a cell-wall type A1γ peptidoglycan	706:739	a cell-wall type A1γ peptidoglycan with meso-diaminopimelic acid	706:769	The strain had a cell-wall type A1γ peptidoglycan with meso-diaminopimelic acid as the diagnostic diamino acid.
29300151	8	19	theme	strain	1085:1090	arg1	7578-1T					1092:1098	strain 7578-1T	1085:1098	strain 7578-1T	1085:1098	The average nucleotide identity values between strain 7578-1T and its most closely related species were 67.8-82.4 % by OrthoANIu analysis.
29300151	2	20	theme	bacterial	116:124	arg1	7578-1T					134:140	7578-1T	134:140	7578-1T	134:140	A novel aerobic, Gram-stain-positive, sporogenous, rod-shaped bacterial strain, 7578-1T, was isolated from ripened Pu'er tea.
29300151	2	20	theme	bacterial	116:124	arg1	strain					126:131	A novel aerobic, Gram-stain-positive, sporogenous, rod-shaped bacterial strain	54:131	A novel aerobic, Gram-stain-positive, sporogenous, rod-shaped bacterial strain	54:131	A novel aerobic, Gram-stain-positive, sporogenous, rod-shaped bacterial strain, 7578-1T, was isolated from ripened Pu'er tea.
29300151	3	21	theme	16S	189:191	arg1	comparisons					223:233	16S rRNA gene sequence similarity comparisons	189:233	16S rRNA gene sequence similarity comparisons	189:233	Based on 16S rRNA gene sequence similarity comparisons, strain 7578-1T was grouped into the genus Bacillus and appeared to be closely related to the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %).
29300151	1	22	theme	er	46:47	arg1	tea					49:51	Pu'er tea	43:51	Pu'er tea	43:51	nov., isolated from Pu'er tea.
29300151	2	23	attach	isolated	147:154	arg2	strain					126:131	A novel aerobic, Gram-stain-positive, sporogenous, rod-shaped bacterial strain	54:131	A novel aerobic, Gram-stain-positive, sporogenous, rod-shaped bacterial strain	54:131	A novel aerobic, Gram-stain-positive, sporogenous, rod-shaped bacterial strain, 7578-1T, was isolated from ripened Pu'er tea.
29300151	2	23	attach	isolated	147:154	arg1	tea					175:177	ripened Pu'er tea	161:177	ripened Pu'er tea	161:177	A novel aerobic, Gram-stain-positive, sporogenous, rod-shaped bacterial strain, 7578-1T, was isolated from ripened Pu'er tea.
29300151	2	23	attach	isolated	147:154	arg2	7578-1T					134:140	7578-1T	134:140	7578-1T	134:140	A novel aerobic, Gram-stain-positive, sporogenous, rod-shaped bacterial strain, 7578-1T, was isolated from ripened Pu'er tea.
29300151	9	24	theme	novel	1343:1347	arg1	species					1349:1355	a novel species	1341:1355	a novel species in the genus Bacillus	1341:1377	The DNA-DNA relatedness value between strain 7578-1T and the type strains of closely related species were 17-39 %, again indicating that strain 7578-1T represented a novel species in the genus Bacillus.
29300151	13	25	theme	1.15374T=KCTC	1658:1670	arg1	7578-1T					1642:1648	7578-1T	1642:1648	7578-1T (=CGMCC 1.15374T=KCTC 33845T)	1642:1678	The type strain is 7578-1T (=CGMCC 1.15374T=KCTC 33845T).
29300151	13	25	theme	1.15374T=KCTC	1658:1670	arg1	33845T					1672:1677	=CGMCC 1.15374T=KCTC 33845T	1651:1677	=CGMCC 1.15374T=KCTC 33845T	1651:1677	The type strain is 7578-1T (=CGMCC 1.15374T=KCTC 33845T).
29300151	3	26	theme	rRNA	193:196	arg1	comparisons					223:233	16S rRNA gene sequence similarity comparisons	189:233	16S rRNA gene sequence similarity comparisons	189:233	Based on 16S rRNA gene sequence similarity comparisons, strain 7578-1T was grouped into the genus Bacillus and appeared to be closely related to the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %).
29300151	5	27	contain	had	702:704	arg1	strain					695:700	The strain	691:700	The strain	691:700	The strain had a cell-wall type A1γ peptidoglycan with meso-diaminopimelic acid as the diagnostic diamino acid.
29300151	5	27	contain	had	702:704	arg2	peptidoglycan					727:739	a cell-wall type A1γ peptidoglycan	706:739	a cell-wall type A1γ peptidoglycan with meso-diaminopimelic acid	706:769	The strain had a cell-wall type A1γ peptidoglycan with meso-diaminopimelic acid as the diagnostic diamino acid.
29300151	5	27	contain	had	702:704	arg2	acid					797:800	the diagnostic diamino acid	774:800	the diagnostic diamino acid	774:800	The strain had a cell-wall type A1γ peptidoglycan with meso-diaminopimelic acid as the diagnostic diamino acid.
29300151	3	28	theme	KACC	450:453	arg1	18426T					455:460	Bacillus paralicheniformis KACC 18426T	423:460	Bacillus paralicheniformis KACC 18426T (97.2 %)	423:469	Based on 16S rRNA gene sequence similarity comparisons, strain 7578-1T was grouped into the genus Bacillus and appeared to be closely related to the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %).
29300151	3	28	theme	KACC	450:453	arg1	%					468:468	97.2 %	463:468	97.2 %	463:468	Based on 16S rRNA gene sequence similarity comparisons, strain 7578-1T was grouped into the genus Bacillus and appeared to be closely related to the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %).
29300151	3	28	theme	KACC	450:453	arg1	strains					334:340	the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %)	325:516	the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %)	325:516	Based on 16S rRNA gene sequence similarity comparisons, strain 7578-1T was grouped into the genus Bacillus and appeared to be closely related to the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %).
29300151	5	29	theme	cell-wall	708:716	arg1	peptidoglycan					727:739	a cell-wall type A1γ peptidoglycan	706:739	a cell-wall type A1γ peptidoglycan with meso-diaminopimelic acid	706:769	The strain had a cell-wall type A1γ peptidoglycan with meso-diaminopimelic acid as the diagnostic diamino acid.
29300151	5	29	theme	cell-wall	708:716	arg1	acid					797:800	the diagnostic diamino acid	774:800	the diagnostic diamino acid	774:800	The strain had a cell-wall type A1γ peptidoglycan with meso-diaminopimelic acid as the diagnostic diamino acid.
29300151	0	30	theme	camelliae	9:17	arg1	sp					19:20	Bacillus camelliae sp	0:20	Bacillus camelliae sp.	0:21	Bacillus camelliae sp.
29300151	8	31	theme	OrthoANIu	1157:1165	arg1	analysis					1167:1174	OrthoANIu analysis	1157:1174	OrthoANIu analysis	1157:1174	The average nucleotide identity values between strain 7578-1T and its most closely related species were 67.8-82.4 % by OrthoANIu analysis.
29300151	3	32	dep	strains	334:340	arg1	13944T					502:507	Bacillus ginsengihumi KCTC 13944T	475:507	Bacillus ginsengihumi KCTC 13944T (96.7 %)	475:516	Based on 16S rRNA gene sequence similarity comparisons, strain 7578-1T was grouped into the genus Bacillus and appeared to be closely related to the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %).
29300151	3	32	dep	strains	334:340	arg1	18435T					367:372	Bacillus shackletoniiLMG 18435T	342:372	Bacillus shackletoniiLMG 18435T (98.4 %)	342:381	Based on 16S rRNA gene sequence similarity comparisons, strain 7578-1T was grouped into the genus Bacillus and appeared to be closely related to the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %).
29300151	3	32	dep	strains	334:340	arg1	%					468:468	97.2 %	463:468	97.2 %	463:468	Based on 16S rRNA gene sequence similarity comparisons, strain 7578-1T was grouped into the genus Bacillus and appeared to be closely related to the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %).
29300151	3	32	dep	strains	334:340	arg1	14745T					406:411	Bacillus acidicolaDSM 14745T	384:411	Bacillus acidicolaDSM 14745T (97.6 %)	384:420	Based on 16S rRNA gene sequence similarity comparisons, strain 7578-1T was grouped into the genus Bacillus and appeared to be closely related to the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %).
29300151	3	32	dep	strains	334:340	arg1	%					515:515	96.7 %	510:515	96.7 %	510:515	Based on 16S rRNA gene sequence similarity comparisons, strain 7578-1T was grouped into the genus Bacillus and appeared to be closely related to the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %).
29300151	3	32	dep	strains	334:340	arg1	%					380:380	98.4 %	375:380	98.4 %	375:380	Based on 16S rRNA gene sequence similarity comparisons, strain 7578-1T was grouped into the genus Bacillus and appeared to be closely related to the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %).
29300151	3	32	dep	strains	334:340	arg1	18426T					455:460	Bacillus paralicheniformis KACC 18426T	423:460	Bacillus paralicheniformis KACC 18426T (97.2 %)	423:469	Based on 16S rRNA gene sequence similarity comparisons, strain 7578-1T was grouped into the genus Bacillus and appeared to be closely related to the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %).
29300151	3	32	dep	strains	334:340	arg1	strains					334:340	the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %)	325:516	the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %)	325:516	Based on 16S rRNA gene sequence similarity comparisons, strain 7578-1T was grouped into the genus Bacillus and appeared to be closely related to the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %).
29300151	3	32	dep	strains	334:340	arg1	%					419:419	97.6 %	414:419	97.6 %	414:419	Based on 16S rRNA gene sequence similarity comparisons, strain 7578-1T was grouped into the genus Bacillus and appeared to be closely related to the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %).
29300151	11	33	theme	polyphasic	1463:1472	arg1	evidence					1474:1481	the presented polyphasic evidence	1449:1481	the presented polyphasic evidence	1449:1481	On the basis of the presented polyphasic evidence, strain 7578-1T is considered to represent a novel species of the genus Bacillus, for which we propose the name Bacillus camelliae sp.
29300151	5	34	theme	type	718:721	arg1	peptidoglycan					727:739	a cell-wall type A1γ peptidoglycan	706:739	a cell-wall type A1γ peptidoglycan with meso-diaminopimelic acid	706:769	The strain had a cell-wall type A1γ peptidoglycan with meso-diaminopimelic acid as the diagnostic diamino acid.
29300151	5	34	theme	type	718:721	arg1	acid					797:800	the diagnostic diamino acid	774:800	the diagnostic diamino acid	774:800	The strain had a cell-wall type A1γ peptidoglycan with meso-diaminopimelic acid as the diagnostic diamino acid.
29300151	3	35	theme	shackletoniiLMG	351:365	arg1	strains					334:340	the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %)	325:516	the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %)	325:516	Based on 16S rRNA gene sequence similarity comparisons, strain 7578-1T was grouped into the genus Bacillus and appeared to be closely related to the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %).
29300151	3	35	theme	shackletoniiLMG	351:365	arg1	18435T					367:372	Bacillus shackletoniiLMG 18435T	342:372	Bacillus shackletoniiLMG 18435T (98.4 %)	342:381	Based on 16S rRNA gene sequence similarity comparisons, strain 7578-1T was grouped into the genus Bacillus and appeared to be closely related to the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %).
29300151	3	35	theme	shackletoniiLMG	351:365	arg1	%					380:380	98.4 %	375:380	98.4 %	375:380	Based on 16S rRNA gene sequence similarity comparisons, strain 7578-1T was grouped into the genus Bacillus and appeared to be closely related to the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %).
29300151	0	36	theme	Bacillus	0:7	arg1	sp					19:20	Bacillus camelliae sp	0:20	Bacillus camelliae sp.	0:21	Bacillus camelliae sp.
29300151	9	37	theme	DNA-DNA	1181:1187	arg1	value					1201:1205	The DNA-DNA relatedness value	1177:1205	The DNA-DNA relatedness value between strain 7578-1T and the type strains of closely related species	1177:1276	The DNA-DNA relatedness value between strain 7578-1T and the type strains of closely related species were 17-39 %, again indicating that strain 7578-1T represented a novel species in the genus Bacillus.
29300151	9	37	theme	DNA-DNA	1181:1187	arg1	%					1289:1289	17-39 %	1283:1289	17-39 %	1283:1289	The DNA-DNA relatedness value between strain 7578-1T and the type strains of closely related species were 17-39 %, again indicating that strain 7578-1T represented a novel species in the genus Bacillus.
29300151	7	38	theme	unidentified	984:995	arg1	phospholipid					997:1008	one unidentified phospholipid	980:1008	one unidentified phospholipid	980:1008	The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, one unidentified phospholipid and one unidentified lipid.
29300151	13	39	theme	type	1627:1630	arg1	7578-1T					1642:1648	7578-1T	1642:1648	7578-1T (=CGMCC 1.15374T=KCTC 33845T)	1642:1678	The type strain is 7578-1T (=CGMCC 1.15374T=KCTC 33845T).
29300151	13	39	theme	type	1627:1630	arg1	strain					1632:1637	The type strain	1623:1637	The type strain	1623:1637	The type strain is 7578-1T (=CGMCC 1.15374T=KCTC 33845T).
29300151	5	40	theme	A1γ	723:725	arg1	peptidoglycan					727:739	a cell-wall type A1γ peptidoglycan	706:739	a cell-wall type A1γ peptidoglycan with meso-diaminopimelic acid	706:769	The strain had a cell-wall type A1γ peptidoglycan with meso-diaminopimelic acid as the diagnostic diamino acid.
29300151	5	40	theme	A1γ	723:725	arg1	acid					797:800	the diagnostic diamino acid	774:800	the diagnostic diamino acid	774:800	The strain had a cell-wall type A1γ peptidoglycan with meso-diaminopimelic acid as the diagnostic diamino acid.
29300151	10	41	theme	DNA	1384:1386	arg1	G+C content					1388:1398	The DNA G+C content	1380:1398	The DNA G+C content of strain 7578-1T	1380:1416	The DNA G+C content of strain 7578-1T was 36.0 mol%.
29300151	10	41	theme	DNA	1384:1386	arg1	%					1430:1430	36.0 mol%	1422:1430	36.0 mol%	1422:1430	The DNA G+C content of strain 7578-1T was 36.0 mol%.
29300151	3	42	theme	similarity	212:221	arg1	comparisons					223:233	16S rRNA gene sequence similarity comparisons	189:233	16S rRNA gene sequence similarity comparisons	189:233	Based on 16S rRNA gene sequence similarity comparisons, strain 7578-1T was grouped into the genus Bacillus and appeared to be closely related to the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %).
29300151	11	43	theme	camelliae	1604:1612	arg1	sp					1614:1615	Bacillus camelliae sp	1595:1615	the name Bacillus camelliae sp	1586:1615	On the basis of the presented polyphasic evidence, strain 7578-1T is considered to represent a novel species of the genus Bacillus, for which we propose the name Bacillus camelliae sp.
29300151	3	44	dep	Bacillus	423:430	arg1	paralicheniformis					432:448	paralicheniformis	432:448	paralicheniformis	432:448	Based on 16S rRNA gene sequence similarity comparisons, strain 7578-1T was grouped into the genus Bacillus and appeared to be closely related to the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %).
29300151	3	45	theme	Bacillus	384:391	arg1	strains					334:340	the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %)	325:516	the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %)	325:516	Based on 16S rRNA gene sequence similarity comparisons, strain 7578-1T was grouped into the genus Bacillus and appeared to be closely related to the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %).
29300151	3	45	theme	Bacillus	384:391	arg1	14745T					406:411	Bacillus acidicolaDSM 14745T	384:411	Bacillus acidicolaDSM 14745T (97.6 %)	384:420	Based on 16S rRNA gene sequence similarity comparisons, strain 7578-1T was grouped into the genus Bacillus and appeared to be closely related to the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %).
29300151	3	45	theme	Bacillus	384:391	arg1	%					419:419	97.6 %	414:419	97.6 %	414:419	Based on 16S rRNA gene sequence similarity comparisons, strain 7578-1T was grouped into the genus Bacillus and appeared to be closely related to the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %).
29300151	8	46	theme	related	1121:1127	arg1	species					1129:1135	its most closely related species	1104:1135	its most closely related species	1104:1135	The average nucleotide identity values between strain 7578-1T and its most closely related species were 67.8-82.4 % by OrthoANIu analysis.
29300151	4	47	theme	major	557:561	arg1	iso-C15 					576:583	iso-C15 	576:583	iso-C15 	576:583	The fatty acid profile containing the major fatty acids, iso-C15 : 0, anteiso-C15 : 0 and anteiso-C17 : 0 supported the allocation of strain 7578-1T to the genus Bacillus.
29300151	4	47	theme	major	557:561	arg1	acids					569:573	the major fatty acids	553:573	the major fatty acids	553:573	The fatty acid profile containing the major fatty acids, iso-C15 : 0, anteiso-C15 : 0 and anteiso-C17 : 0 supported the allocation of strain 7578-1T to the genus Bacillus.
29300151	3	48	theme	gene	198:201	arg1	comparisons					223:233	16S rRNA gene sequence similarity comparisons	189:233	16S rRNA gene sequence similarity comparisons	189:233	Based on 16S rRNA gene sequence similarity comparisons, strain 7578-1T was grouped into the genus Bacillus and appeared to be closely related to the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %).
29300151	3	49	theme	KCTC	497:500	arg1	strains					334:340	the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %)	325:516	the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %)	325:516	Based on 16S rRNA gene sequence similarity comparisons, strain 7578-1T was grouped into the genus Bacillus and appeared to be closely related to the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %).
29300151	3	49	theme	KCTC	497:500	arg1	13944T					502:507	Bacillus ginsengihumi KCTC 13944T	475:507	Bacillus ginsengihumi KCTC 13944T (96.7 %)	475:516	Based on 16S rRNA gene sequence similarity comparisons, strain 7578-1T was grouped into the genus Bacillus and appeared to be closely related to the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %).
29300151	3	49	theme	KCTC	497:500	arg1	%					515:515	96.7 %	510:515	96.7 %	510:515	Based on 16S rRNA gene sequence similarity comparisons, strain 7578-1T was grouped into the genus Bacillus and appeared to be closely related to the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %).
29300151	4	50	theme	acid	529:532	arg1	profile					534:540	The fatty acid profile	519:540	The fatty acid profile containing the major fatty acids, iso-C15 : 0, anteiso-C15 : 0 and anteiso-C17 : 0	519:623	The fatty acid profile containing the major fatty acids, iso-C15 : 0, anteiso-C15 : 0 and anteiso-C17 : 0 supported the allocation of strain 7578-1T to the genus Bacillus.
29300151	3	51	theme	Bacillus	342:349	arg1	strains					334:340	the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %)	325:516	the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %)	325:516	Based on 16S rRNA gene sequence similarity comparisons, strain 7578-1T was grouped into the genus Bacillus and appeared to be closely related to the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %).
29300151	3	51	theme	Bacillus	342:349	arg1	18435T					367:372	Bacillus shackletoniiLMG 18435T	342:372	Bacillus shackletoniiLMG 18435T (98.4 %)	342:381	Based on 16S rRNA gene sequence similarity comparisons, strain 7578-1T was grouped into the genus Bacillus and appeared to be closely related to the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %).
29300151	3	51	theme	Bacillus	342:349	arg1	%					380:380	98.4 %	375:380	98.4 %	375:380	Based on 16S rRNA gene sequence similarity comparisons, strain 7578-1T was grouped into the genus Bacillus and appeared to be closely related to the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %).
29300151	2	52	theme	aerobic	62:68	arg1	7578-1T					134:140	7578-1T	134:140	7578-1T	134:140	A novel aerobic, Gram-stain-positive, sporogenous, rod-shaped bacterial strain, 7578-1T, was isolated from ripened Pu'er tea.
29300151	2	52	theme	aerobic	62:68	arg1	strain					126:131	A novel aerobic, Gram-stain-positive, sporogenous, rod-shaped bacterial strain	54:131	A novel aerobic, Gram-stain-positive, sporogenous, rod-shaped bacterial strain	54:131	A novel aerobic, Gram-stain-positive, sporogenous, rod-shaped bacterial strain, 7578-1T, was isolated from ripened Pu'er tea.
29300151	3	53	theme	sequence	203:210	arg1	comparisons					223:233	16S rRNA gene sequence similarity comparisons	189:233	16S rRNA gene sequence similarity comparisons	189:233	Based on 16S rRNA gene sequence similarity comparisons, strain 7578-1T was grouped into the genus Bacillus and appeared to be closely related to the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %).
29300151	11	54	theme	novel	1528:1532	arg1	species					1534:1540	a novel species	1526:1540	a novel species	1526:1540	On the basis of the presented polyphasic evidence, strain 7578-1T is considered to represent a novel species of the genus Bacillus, for which we propose the name Bacillus camelliae sp.
29300151	7	55	theme	polar	858:862	arg1	diphosphatidylglycerol					876:897	diphosphatidylglycerol	876:897	diphosphatidylglycerol	876:897	The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, one unidentified phospholipid and one unidentified lipid.
29300151	7	55	theme	polar	858:862	arg1	lipids					864:869	The predominant polar lipids	842:869	The predominant polar lipids	842:869	The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, one unidentified phospholipid and one unidentified lipid.
29300151	4	56	theme	fatty	523:527	arg1	profile					534:540	The fatty acid profile	519:540	The fatty acid profile containing the major fatty acids, iso-C15 : 0, anteiso-C15 : 0 and anteiso-C17 : 0	519:623	The fatty acid profile containing the major fatty acids, iso-C15 : 0, anteiso-C15 : 0 and anteiso-C17 : 0 supported the allocation of strain 7578-1T to the genus Bacillus.
29300151	2	57	theme	novel	56:60	arg1	7578-1T					134:140	7578-1T	134:140	7578-1T	134:140	A novel aerobic, Gram-stain-positive, sporogenous, rod-shaped bacterial strain, 7578-1T, was isolated from ripened Pu'er tea.
29300151	2	57	theme	novel	56:60	arg1	strain					126:131	A novel aerobic, Gram-stain-positive, sporogenous, rod-shaped bacterial strain	54:131	A novel aerobic, Gram-stain-positive, sporogenous, rod-shaped bacterial strain	54:131	A novel aerobic, Gram-stain-positive, sporogenous, rod-shaped bacterial strain, 7578-1T, was isolated from ripened Pu'er tea.
29300151	10	58	theme	strain	1403:1408	arg1	7578-1T					1410:1416	strain 7578-1T	1403:1416	strain 7578-1T	1403:1416	The DNA G+C content of strain 7578-1T was 36.0 mol%.
29300151	3	59	theme	Bacillus	475:482	arg1	strains					334:340	the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %)	325:516	the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %)	325:516	Based on 16S rRNA gene sequence similarity comparisons, strain 7578-1T was grouped into the genus Bacillus and appeared to be closely related to the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %).
29300151	3	59	theme	Bacillus	475:482	arg1	13944T					502:507	Bacillus ginsengihumi KCTC 13944T	475:507	Bacillus ginsengihumi KCTC 13944T (96.7 %)	475:516	Based on 16S rRNA gene sequence similarity comparisons, strain 7578-1T was grouped into the genus Bacillus and appeared to be closely related to the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %).
29300151	3	59	theme	Bacillus	475:482	arg1	%					515:515	96.7 %	510:515	96.7 %	510:515	Based on 16S rRNA gene sequence similarity comparisons, strain 7578-1T was grouped into the genus Bacillus and appeared to be closely related to the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %).
29300151	11	60	dep	evidence	1474:1481	arg1	the					1436:1438	the	1436:1438	the	1436:1438	On the basis of the presented polyphasic evidence, strain 7578-1T is considered to represent a novel species of the genus Bacillus, for which we propose the name Bacillus camelliae sp.
29300151	11	60	dep	evidence	1474:1481	arg1	basis					1440:1444	basis	1440:1444	basis	1440:1444	On the basis of the presented polyphasic evidence, strain 7578-1T is considered to represent a novel species of the genus Bacillus, for which we propose the name Bacillus camelliae sp.
29300151	3	61	theme	type	329:332	arg1	18426T					455:460	Bacillus paralicheniformis KACC 18426T	423:460	Bacillus paralicheniformis KACC 18426T (97.2 %)	423:469	Based on 16S rRNA gene sequence similarity comparisons, strain 7578-1T was grouped into the genus Bacillus and appeared to be closely related to the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %).
29300151	3	61	theme	type	329:332	arg1	13944T					502:507	Bacillus ginsengihumi KCTC 13944T	475:507	Bacillus ginsengihumi KCTC 13944T (96.7 %)	475:516	Based on 16S rRNA gene sequence similarity comparisons, strain 7578-1T was grouped into the genus Bacillus and appeared to be closely related to the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %).
29300151	3	61	theme	type	329:332	arg1	strains					334:340	the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %)	325:516	the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %)	325:516	Based on 16S rRNA gene sequence similarity comparisons, strain 7578-1T was grouped into the genus Bacillus and appeared to be closely related to the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %).
29300151	3	61	theme	type	329:332	arg1	14745T					406:411	Bacillus acidicolaDSM 14745T	384:411	Bacillus acidicolaDSM 14745T (97.6 %)	384:420	Based on 16S rRNA gene sequence similarity comparisons, strain 7578-1T was grouped into the genus Bacillus and appeared to be closely related to the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %).
29300151	3	61	theme	type	329:332	arg1	18435T					367:372	Bacillus shackletoniiLMG 18435T	342:372	Bacillus shackletoniiLMG 18435T (98.4 %)	342:381	Based on 16S rRNA gene sequence similarity comparisons, strain 7578-1T was grouped into the genus Bacillus and appeared to be closely related to the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %).
29300151	10	62	theme	36.0 mol	1422:1429	arg1	G+C content					1388:1398	The DNA G+C content	1380:1398	The DNA G+C content of strain 7578-1T	1380:1416	The DNA G+C content of strain 7578-1T was 36.0 mol%.
29300151	10	62	theme	36.0 mol	1422:1429	arg1	%					1430:1430	36.0 mol%	1422:1430	36.0 mol%	1422:1430	The DNA G+C content of strain 7578-1T was 36.0 mol%.
29300151	2	63	theme	Pu	169:170	arg1	tea					175:177	ripened Pu'er tea	161:177	ripened Pu'er tea	161:177	A novel aerobic, Gram-stain-positive, sporogenous, rod-shaped bacterial strain, 7578-1T, was isolated from ripened Pu'er tea.
29300151	3	64	theme	ginsengihumi	484:495	arg1	strains					334:340	the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %)	325:516	the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %)	325:516	Based on 16S rRNA gene sequence similarity comparisons, strain 7578-1T was grouped into the genus Bacillus and appeared to be closely related to the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %).
29300151	3	64	theme	ginsengihumi	484:495	arg1	13944T					502:507	Bacillus ginsengihumi KCTC 13944T	475:507	Bacillus ginsengihumi KCTC 13944T (96.7 %)	475:516	Based on 16S rRNA gene sequence similarity comparisons, strain 7578-1T was grouped into the genus Bacillus and appeared to be closely related to the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %).
29300151	3	64	theme	ginsengihumi	484:495	arg1	%					515:515	96.7 %	510:515	96.7 %	510:515	Based on 16S rRNA gene sequence similarity comparisons, strain 7578-1T was grouped into the genus Bacillus and appeared to be closely related to the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %).
29300151	6	65	theme	major	807:811	arg1	MK-7					829:832	MK-7	829:832	MK-7	829:832	The major menaquinone was MK-7 (95 %).
29300151	6	65	theme	major	807:811	arg1	menaquinone					813:823	The major menaquinone	803:823	The major menaquinone	803:823	The major menaquinone was MK-7 (95 %).
29300151	2	66	theme	ripened	161:167	arg1	tea					175:177	ripened Pu'er tea	161:177	ripened Pu'er tea	161:177	A novel aerobic, Gram-stain-positive, sporogenous, rod-shaped bacterial strain, 7578-1T, was isolated from ripened Pu'er tea.
29300151	9	67	theme	related	1262:1268	arg1	species					1270:1276	closely related species	1254:1276	closely related species	1254:1276	The DNA-DNA relatedness value between strain 7578-1T and the type strains of closely related species were 17-39 %, again indicating that strain 7578-1T represented a novel species in the genus Bacillus.
29300151	7	68	theme	predominant	846:856	arg1	diphosphatidylglycerol					876:897	diphosphatidylglycerol	876:897	diphosphatidylglycerol	876:897	The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, one unidentified phospholipid and one unidentified lipid.
29300151	7	68	theme	predominant	846:856	arg1	lipids					864:869	The predominant polar lipids	842:869	The predominant polar lipids	842:869	The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, one unidentified phospholipid and one unidentified lipid.
29300151	3	69	theme	acidicolaDSM	393:404	arg1	strains					334:340	the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %)	325:516	the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %)	325:516	Based on 16S rRNA gene sequence similarity comparisons, strain 7578-1T was grouped into the genus Bacillus and appeared to be closely related to the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %).
29300151	3	69	theme	acidicolaDSM	393:404	arg1	14745T					406:411	Bacillus acidicolaDSM 14745T	384:411	Bacillus acidicolaDSM 14745T (97.6 %)	384:420	Based on 16S rRNA gene sequence similarity comparisons, strain 7578-1T was grouped into the genus Bacillus and appeared to be closely related to the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %).
29300151	3	69	theme	acidicolaDSM	393:404	arg1	%					419:419	97.6 %	414:419	97.6 %	414:419	Based on 16S rRNA gene sequence similarity comparisons, strain 7578-1T was grouped into the genus Bacillus and appeared to be closely related to the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %).
29300151	3	70	theme	strain	236:241	arg1	7578-1T					243:249	strain 7578-1T	236:249	strain 7578-1T	236:249	Based on 16S rRNA gene sequence similarity comparisons, strain 7578-1T was grouped into the genus Bacillus and appeared to be closely related to the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %).
29300151	11	71	theme	strain	1484:1489	arg1	7578-1T					1491:1497	strain 7578-1T	1484:1497	strain 7578-1T	1484:1497	On the basis of the presented polyphasic evidence, strain 7578-1T is considered to represent a novel species of the genus Bacillus, for which we propose the name Bacillus camelliae sp.
29300151	4	72	theme	fatty	563:567	arg1	iso-C15 					576:583	iso-C15 	576:583	iso-C15 	576:583	The fatty acid profile containing the major fatty acids, iso-C15 : 0, anteiso-C15 : 0 and anteiso-C17 : 0 supported the allocation of strain 7578-1T to the genus Bacillus.
29300151	4	72	theme	fatty	563:567	arg1	acids					569:573	the major fatty acids	553:573	the major fatty acids	553:573	The fatty acid profile containing the major fatty acids, iso-C15 : 0, anteiso-C15 : 0 and anteiso-C17 : 0 supported the allocation of strain 7578-1T to the genus Bacillus.
29300151	9	73	theme	species	1270:1276	arg1	strains					1243:1249	the type strains	1234:1249	the type strains of closely related species	1234:1276	The DNA-DNA relatedness value between strain 7578-1T and the type strains of closely related species were 17-39 %, again indicating that strain 7578-1T represented a novel species in the genus Bacillus.
29300151	9	73	theme	species	1270:1276	arg1	7578-1T					1222:1228	strain 7578-1T	1215:1228	strain 7578-1T	1215:1228	The DNA-DNA relatedness value between strain 7578-1T and the type strains of closely related species were 17-39 %, again indicating that strain 7578-1T represented a novel species in the genus Bacillus.
29300151	5	74	theme	diagnostic	778:787	arg1	acid					797:800	the diagnostic diamino acid	774:800	the diagnostic diamino acid	774:800	The strain had a cell-wall type A1γ peptidoglycan with meso-diaminopimelic acid as the diagnostic diamino acid.
29300151	5	74	theme	diagnostic	778:787	arg1	peptidoglycan					727:739	a cell-wall type A1γ peptidoglycan	706:739	a cell-wall type A1γ peptidoglycan with meso-diaminopimelic acid	706:769	The strain had a cell-wall type A1γ peptidoglycan with meso-diaminopimelic acid as the diagnostic diamino acid.
29300151	4	75	contain	containing	542:551	arg2	iso-C15 					576:583	iso-C15 	576:583	iso-C15 	576:583	The fatty acid profile containing the major fatty acids, iso-C15 : 0, anteiso-C15 : 0 and anteiso-C17 : 0 supported the allocation of strain 7578-1T to the genus Bacillus.
29300151	4	75	contain	containing	542:551	arg2	acids					569:573	the major fatty acids	553:573	the major fatty acids	553:573	The fatty acid profile containing the major fatty acids, iso-C15 : 0, anteiso-C15 : 0 and anteiso-C17 : 0 supported the allocation of strain 7578-1T to the genus Bacillus.
29300151	4	75	contain	containing	542:551	arg1	profile					534:540	The fatty acid profile	519:540	The fatty acid profile containing the major fatty acids, iso-C15 : 0, anteiso-C15 : 0 and anteiso-C17 : 0	519:623	The fatty acid profile containing the major fatty acids, iso-C15 : 0, anteiso-C15 : 0 and anteiso-C17 : 0 supported the allocation of strain 7578-1T to the genus Bacillus.
29300151	4	76	theme	7578-1T	660:666	arg1	allocation					639:648	the allocation	635:648	the allocation of strain 7578-1T to the genus Bacillus	635:688	The fatty acid profile containing the major fatty acids, iso-C15 : 0, anteiso-C15 : 0 and anteiso-C17 : 0 supported the allocation of strain 7578-1T to the genus Bacillus.
29300151	9	77	theme	relatedness	1189:1199	arg1	value					1201:1205	The DNA-DNA relatedness value	1177:1205	The DNA-DNA relatedness value between strain 7578-1T and the type strains of closely related species	1177:1276	The DNA-DNA relatedness value between strain 7578-1T and the type strains of closely related species were 17-39 %, again indicating that strain 7578-1T represented a novel species in the genus Bacillus.
29300151	9	77	theme	relatedness	1189:1199	arg1	%					1289:1289	17-39 %	1283:1289	17-39 %	1283:1289	The DNA-DNA relatedness value between strain 7578-1T and the type strains of closely related species were 17-39 %, again indicating that strain 7578-1T represented a novel species in the genus Bacillus.
29300151	4	78	theme	strain	653:658	arg1	7578-1T					660:666	strain 7578-1T	653:666	strain 7578-1T	653:666	The fatty acid profile containing the major fatty acids, iso-C15 : 0, anteiso-C15 : 0 and anteiso-C17 : 0 supported the allocation of strain 7578-1T to the genus Bacillus.
29300151	3	79	theme	Bacillus	423:430	arg1	18426T					455:460	Bacillus paralicheniformis KACC 18426T	423:460	Bacillus paralicheniformis KACC 18426T (97.2 %)	423:469	Based on 16S rRNA gene sequence similarity comparisons, strain 7578-1T was grouped into the genus Bacillus and appeared to be closely related to the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %).
29300151	3	79	theme	Bacillus	423:430	arg1	%					468:468	97.2 %	463:468	97.2 %	463:468	Based on 16S rRNA gene sequence similarity comparisons, strain 7578-1T was grouped into the genus Bacillus and appeared to be closely related to the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %).
29300151	3	79	theme	Bacillus	423:430	arg1	strains					334:340	the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %)	325:516	the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %)	325:516	Based on 16S rRNA gene sequence similarity comparisons, strain 7578-1T was grouped into the genus Bacillus and appeared to be closely related to the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %).
29300151	10	80	theme	7578-1T	1410:1416	arg1	G+C content					1388:1398	The DNA G+C content	1380:1398	The DNA G+C content of strain 7578-1T	1380:1416	The DNA G+C content of strain 7578-1T was 36.0 mol%.
29300151	10	80	theme	7578-1T	1410:1416	arg1	%					1430:1430	36.0 mol%	1422:1430	36.0 mol%	1422:1430	The DNA G+C content of strain 7578-1T was 36.0 mol%.
29581723	1	0	theme	polysaccharides	246:260	arg1	UAE					238:240	UAE	238:240	UAE	238:240	Response surface methodology (RSM) was employed to optimize the conditions for the ultrasonic-assisted extraction (UAE) of polysaccharides from the flowers of Dendrobium devonianum.
29581723	1	0	theme	polysaccharides	246:260	arg1	extraction					226:235	the ultrasonic-assisted extraction	202:235	the ultrasonic-assisted extraction (UAE) of polysaccharides from the flowers of Dendrobium devonianum	202:302	Response surface methodology (RSM) was employed to optimize the conditions for the ultrasonic-assisted extraction (UAE) of polysaccharides from the flowers of Dendrobium devonianum.
29581723	2	1	theme	material	466:473	arg1	ratio					475:479	a water-to-raw material ratio	451:479	a water-to-raw material ratio of 22.11 mL/g	451:493	The optimal conditions for the maximum yields of DDFPs are as follows: an extraction temperature of 63.13°C, an extraction time of 53.10 min, and a water-to-raw material ratio of 22.11 mL/g.
29581723	2	2	theme	DDFPs	354:358	arg1	yields					344:349	the maximum yields	332:349	the maximum yields of DDFPs	332:358	The optimal conditions for the maximum yields of DDFPs are as follows: an extraction temperature of 63.13°C, an extraction time of 53.10 min, and a water-to-raw material ratio of 22.11 mL/g.
29581723	6	3	theme	similar	994:1000	arg1	mannose					946:952	mannose	946:952	mannose	946:952	In particular, the monosaccharide composition analysis of the DDFPs indicates that mannose and glucose are the primary components, similar to those of the D. officinale plant.
29581723	6	3	theme	similar	994:1000	arg1	components					982:991	the primary components	970:991	the primary components	970:991	In particular, the monosaccharide composition analysis of the DDFPs indicates that mannose and glucose are the primary components, similar to those of the D. officinale plant.
29581723	6	3	theme	similar	994:1000	arg1	glucose					958:964	glucose	958:964	glucose	958:964	In particular, the monosaccharide composition analysis of the DDFPs indicates that mannose and glucose are the primary components, similar to those of the D. officinale plant.
29581723	0	4	theme	Dendrobium	100:109	arg1	devonianum					111:120	Dendrobium devonianum	100:120	Dendrobium devonianum	100:120	Optimum Extraction, Characterization, and Antioxidant Activities of Polysaccharides from Flowers of Dendrobium devonianum.
29581723	7	5	theme	healthcare	1179:1188	arg1	food					1190:1193	healthcare food	1179:1193	healthcare food	1179:1193	This study provides a rapid extraction technology and essential information for the production of DDFPs, which could be potentially used as healthcare food.
29581723	7	5	theme	healthcare	1179:1188	arg1	technology					1078:1087	a rapid extraction technology	1059:1087	a rapid extraction technology	1059:1087	This study provides a rapid extraction technology and essential information for the production of DDFPs, which could be potentially used as healthcare food.
29581723	7	5	theme	healthcare	1179:1188	arg1	information					1103:1113	essential information	1093:1113	essential information	1093:1113	This study provides a rapid extraction technology and essential information for the production of DDFPs, which could be potentially used as healthcare food.
29581723	6	6	theme	composition	897:907	arg1	analysis					909:916	the monosaccharide composition analysis	878:916	the monosaccharide composition analysis of the DDFPs	878:929	In particular, the monosaccharide composition analysis of the DDFPs indicates that mannose and glucose are the primary components, similar to those of the D. officinale plant.
29581723	4	7	theme	other	747:751	arg1	fractions					753:761	the other fractions	743:761	the other fractions	743:761	The DDFPs50 exhibited the highest antioxidant activity compared to the other fractions.
29581723	3	8	dep	fractions	515:523	arg1	DDFPs30					526:532	DDFPs30	526:532	DDFPs30	526:532	Furthermore, three fractions (DDFPs30, DDFPs50, and DDFPs70) were prepared from Dendrobium devonianum flowers polysaccharides (DDFPs) by the stepwise ethanol precipitation method.
29581723	3	8	dep	fractions	515:523	arg1	fractions					515:523	three fractions	509:523	three fractions (DDFPs30, DDFPs50, and DDFPs70)	509:555	Furthermore, three fractions (DDFPs30, DDFPs50, and DDFPs70) were prepared from Dendrobium devonianum flowers polysaccharides (DDFPs) by the stepwise ethanol precipitation method.
29581723	3	8	dep	fractions	515:523	arg1	DDFPs50					535:541	DDFPs50	535:541	DDFPs50	535:541	Furthermore, three fractions (DDFPs30, DDFPs50, and DDFPs70) were prepared from Dendrobium devonianum flowers polysaccharides (DDFPs) by the stepwise ethanol precipitation method.
29581723	3	8	dep	fractions	515:523	arg1	DDFPs70					548:554	DDFPs70	548:554	DDFPs70	548:554	Furthermore, three fractions (DDFPs30, DDFPs50, and DDFPs70) were prepared from Dendrobium devonianum flowers polysaccharides (DDFPs) by the stepwise ethanol precipitation method.
29581723	0	9	theme	devonianum	111:120	arg1	Flowers					89:95	Flowers	89:95	Flowers of Dendrobium devonianum	89:120	Optimum Extraction, Characterization, and Antioxidant Activities of Polysaccharides from Flowers of Dendrobium devonianum.
29581723	5	10	theme	molecular	768:776	arg1	weight					778:783	The molecular weight	764:783	The molecular weight	764:783	The molecular weight, polydispersity, and conformation of these fractions were also characterized.
29581723	7	11	theme	rapid	1061:1065	arg1	food					1190:1193	healthcare food	1179:1193	healthcare food	1179:1193	This study provides a rapid extraction technology and essential information for the production of DDFPs, which could be potentially used as healthcare food.
29581723	7	11	theme	rapid	1061:1065	arg1	technology					1078:1087	a rapid extraction technology	1059:1087	a rapid extraction technology	1059:1087	This study provides a rapid extraction technology and essential information for the production of DDFPs, which could be potentially used as healthcare food.
29581723	7	11	theme	rapid	1061:1065	arg1	information					1103:1113	essential information	1093:1113	essential information	1093:1113	This study provides a rapid extraction technology and essential information for the production of DDFPs, which could be potentially used as healthcare food.
29581723	2	12	theme	extraction	379:388	arg1	temperature					390:400	an extraction temperature	376:400	an extraction temperature of 63.13°C	376:411	The optimal conditions for the maximum yields of DDFPs are as follows: an extraction temperature of 63.13°C, an extraction time of 53.10 min, and a water-to-raw material ratio of 22.11 mL/g.
29581723	2	13	theme	53.10 min	436:444	arg1	time					428:431	an extraction time	414:431	an extraction time of 53.10 min	414:444	The optimal conditions for the maximum yields of DDFPs are as follows: an extraction temperature of 63.13°C, an extraction time of 53.10 min, and a water-to-raw material ratio of 22.11 mL/g.
29581723	7	14	theme	extraction	1067:1076	arg1	food					1190:1193	healthcare food	1179:1193	healthcare food	1179:1193	This study provides a rapid extraction technology and essential information for the production of DDFPs, which could be potentially used as healthcare food.
29581723	7	14	theme	extraction	1067:1076	arg1	technology					1078:1087	a rapid extraction technology	1059:1087	a rapid extraction technology	1059:1087	This study provides a rapid extraction technology and essential information for the production of DDFPs, which could be potentially used as healthcare food.
29581723	7	14	theme	extraction	1067:1076	arg1	information					1103:1113	essential information	1093:1113	essential information	1093:1113	This study provides a rapid extraction technology and essential information for the production of DDFPs, which could be potentially used as healthcare food.
29581723	0	15	theme	Polysaccharides	68:82	arg1	Characterization					20:35	Characterization	20:35	Characterization	20:35	Optimum Extraction, Characterization, and Antioxidant Activities of Polysaccharides from Flowers of Dendrobium devonianum.
29581723	0	15	theme	Polysaccharides	68:82	arg1	Extraction					8:17	Optimum Extraction	0:17	Optimum Extraction	0:17	Optimum Extraction, Characterization, and Antioxidant Activities of Polysaccharides from Flowers of Dendrobium devonianum.
29581723	0	15	theme	Polysaccharides	68:82	arg1	Activities					54:63	Antioxidant Activities	42:63	Antioxidant Activities	42:63	Optimum Extraction, Characterization, and Antioxidant Activities of Polysaccharides from Flowers of Dendrobium devonianum.
29581723	6	16	theme	monosaccharide	882:895	arg1	analysis					909:916	the monosaccharide composition analysis	878:916	the monosaccharide composition analysis of the DDFPs	878:929	In particular, the monosaccharide composition analysis of the DDFPs indicates that mannose and glucose are the primary components, similar to those of the D. officinale plant.
29581723	0	17	theme	Optimum	0:6	arg1	Extraction					8:17	Optimum Extraction	0:17	Optimum Extraction	0:17	Optimum Extraction, Characterization, and Antioxidant Activities of Polysaccharides from Flowers of Dendrobium devonianum.
29581723	6	18	theme	primary	974:980	arg1	mannose					946:952	mannose	946:952	mannose	946:952	In particular, the monosaccharide composition analysis of the DDFPs indicates that mannose and glucose are the primary components, similar to those of the D. officinale plant.
29581723	6	18	theme	primary	974:980	arg1	components					982:991	the primary components	970:991	the primary components	970:991	In particular, the monosaccharide composition analysis of the DDFPs indicates that mannose and glucose are the primary components, similar to those of the D. officinale plant.
29581723	6	18	theme	primary	974:980	arg1	glucose					958:964	glucose	958:964	glucose	958:964	In particular, the monosaccharide composition analysis of the DDFPs indicates that mannose and glucose are the primary components, similar to those of the D. officinale plant.
29581723	1	19	theme	Dendrobium	282:291	arg1	devonianum					293:302	Dendrobium devonianum	282:302	Dendrobium devonianum	282:302	Response surface methodology (RSM) was employed to optimize the conditions for the ultrasonic-assisted extraction (UAE) of polysaccharides from the flowers of Dendrobium devonianum.
29581723	2	20	theme	optimal	309:315	arg1	conditions					317:326	The optimal conditions	305:326	The optimal conditions for the maximum yields of DDFPs	305:358	The optimal conditions for the maximum yields of DDFPs are as follows: an extraction temperature of 63.13°C, an extraction time of 53.10 min, and a water-to-raw material ratio of 22.11 mL/g.
29581723	2	21	theme	extraction	417:426	arg1	time					428:431	an extraction time	414:431	an extraction time of 53.10 min	414:444	The optimal conditions for the maximum yields of DDFPs are as follows: an extraction temperature of 63.13°C, an extraction time of 53.10 min, and a water-to-raw material ratio of 22.11 mL/g.
29581723	1	22	theme	devonianum	293:302	arg1	flowers					271:277	the flowers	267:277	the flowers of Dendrobium devonianum	267:302	Response surface methodology (RSM) was employed to optimize the conditions for the ultrasonic-assisted extraction (UAE) of polysaccharides from the flowers of Dendrobium devonianum.
29581723	1	23	from	flowers	271:277	arg1	UAE					238:240	UAE	238:240	UAE	238:240	Response surface methodology (RSM) was employed to optimize the conditions for the ultrasonic-assisted extraction (UAE) of polysaccharides from the flowers of Dendrobium devonianum.
29581723	1	23	from	flowers	271:277	arg1	polysaccharides					246:260	polysaccharides	246:260	polysaccharides from the flowers of Dendrobium devonianum	246:302	Response surface methodology (RSM) was employed to optimize the conditions for the ultrasonic-assisted extraction (UAE) of polysaccharides from the flowers of Dendrobium devonianum.
29581723	1	23	from	flowers	271:277	arg1	extraction					226:235	the ultrasonic-assisted extraction	202:235	the ultrasonic-assisted extraction (UAE) of polysaccharides from the flowers of Dendrobium devonianum	202:302	Response surface methodology (RSM) was employed to optimize the conditions for the ultrasonic-assisted extraction (UAE) of polysaccharides from the flowers of Dendrobium devonianum.
29581723	0	24	from	Flowers	89:95	arg1	Characterization					20:35	Characterization	20:35	Characterization	20:35	Optimum Extraction, Characterization, and Antioxidant Activities of Polysaccharides from Flowers of Dendrobium devonianum.
29581723	0	24	from	Flowers	89:95	arg1	Extraction					8:17	Optimum Extraction	0:17	Optimum Extraction	0:17	Optimum Extraction, Characterization, and Antioxidant Activities of Polysaccharides from Flowers of Dendrobium devonianum.
29581723	0	24	from	Flowers	89:95	arg1	Activities					54:63	Antioxidant Activities	42:63	Antioxidant Activities	42:63	Optimum Extraction, Characterization, and Antioxidant Activities of Polysaccharides from Flowers of Dendrobium devonianum.
29581723	5	25	theme	fractions	828:836	arg1	polydispersity					786:799	polydispersity	786:799	polydispersity	786:799	The molecular weight, polydispersity, and conformation of these fractions were also characterized.
29581723	5	25	theme	fractions	828:836	arg1	conformation					806:817	conformation	806:817	conformation	806:817	The molecular weight, polydispersity, and conformation of these fractions were also characterized.
29581723	5	25	theme	fractions	828:836	arg1	weight					778:783	The molecular weight	764:783	The molecular weight	764:783	The molecular weight, polydispersity, and conformation of these fractions were also characterized.
29581723	2	26	theme	water-to-raw	453:464	arg1	ratio					475:479	a water-to-raw material ratio	451:479	a water-to-raw material ratio of 22.11 mL/g	451:493	The optimal conditions for the maximum yields of DDFPs are as follows: an extraction temperature of 63.13°C, an extraction time of 53.10 min, and a water-to-raw material ratio of 22.11 mL/g.
29581723	7	27	used	used	1171:1174	arg2	information					1103:1113	essential information	1093:1113	essential information	1093:1113	This study provides a rapid extraction technology and essential information for the production of DDFPs, which could be potentially used as healthcare food.
29581723	7	27	used	used	1171:1174	arg2	technology					1078:1087	a rapid extraction technology	1059:1087	a rapid extraction technology	1059:1087	This study provides a rapid extraction technology and essential information for the production of DDFPs, which could be potentially used as healthcare food.
29581723	7	27	used	used	1171:1174	arg2	food					1190:1193	healthcare food	1179:1193	healthcare food	1179:1193	This study provides a rapid extraction technology and essential information for the production of DDFPs, which could be potentially used as healthcare food.
29581723	1	28	theme	ultrasonic-assisted	206:224	arg1	UAE					238:240	UAE	238:240	UAE	238:240	Response surface methodology (RSM) was employed to optimize the conditions for the ultrasonic-assisted extraction (UAE) of polysaccharides from the flowers of Dendrobium devonianum.
29581723	1	28	theme	ultrasonic-assisted	206:224	arg1	extraction					226:235	the ultrasonic-assisted extraction	202:235	the ultrasonic-assisted extraction (UAE) of polysaccharides from the flowers of Dendrobium devonianum	202:302	Response surface methodology (RSM) was employed to optimize the conditions for the ultrasonic-assisted extraction (UAE) of polysaccharides from the flowers of Dendrobium devonianum.
29581723	2	29	theme	63.13°C	405:411	arg1	temperature					390:400	an extraction temperature	376:400	an extraction temperature of 63.13°C	376:411	The optimal conditions for the maximum yields of DDFPs are as follows: an extraction temperature of 63.13°C, an extraction time of 53.10 min, and a water-to-raw material ratio of 22.11 mL/g.
29581723	6	30	theme	officinale	1021:1030	arg1	plant					1032:1036	the D. officinale plant	1014:1036	the D. officinale plant	1014:1036	In particular, the monosaccharide composition analysis of the DDFPs indicates that mannose and glucose are the primary components, similar to those of the D. officinale plant.
29581723	1	31	from	extraction	226:235	arg1	flowers					271:277	the flowers	267:277	the flowers of Dendrobium devonianum	267:302	Response surface methodology (RSM) was employed to optimize the conditions for the ultrasonic-assisted extraction (UAE) of polysaccharides from the flowers of Dendrobium devonianum.
29581723	1	32	theme	Response	123:130	arg1	methodology					140:150	Response surface methodology	123:150	Response surface methodology (RSM)	123:156	Response surface methodology (RSM) was employed to optimize the conditions for the ultrasonic-assisted extraction (UAE) of polysaccharides from the flowers of Dendrobium devonianum.
29581723	1	32	theme	Response	123:130	arg1	RSM					153:155	RSM	153:155	RSM	153:155	Response surface methodology (RSM) was employed to optimize the conditions for the ultrasonic-assisted extraction (UAE) of polysaccharides from the flowers of Dendrobium devonianum.
29581723	0	33	theme	Antioxidant	42:52	arg1	Activities					54:63	Antioxidant Activities	42:63	Antioxidant Activities	42:63	Optimum Extraction, Characterization, and Antioxidant Activities of Polysaccharides from Flowers of Dendrobium devonianum.
29581723	3	34	theme	ethanol	646:652	arg1	method					668:673	the stepwise ethanol precipitation method	633:673	the stepwise ethanol precipitation method	633:673	Furthermore, three fractions (DDFPs30, DDFPs50, and DDFPs70) were prepared from Dendrobium devonianum flowers polysaccharides (DDFPs) by the stepwise ethanol precipitation method.
29581723	7	35	theme	essential	1093:1101	arg1	food					1190:1193	healthcare food	1179:1193	healthcare food	1179:1193	This study provides a rapid extraction technology and essential information for the production of DDFPs, which could be potentially used as healthcare food.
29581723	7	35	theme	essential	1093:1101	arg1	technology					1078:1087	a rapid extraction technology	1059:1087	a rapid extraction technology	1059:1087	This study provides a rapid extraction technology and essential information for the production of DDFPs, which could be potentially used as healthcare food.
29581723	7	35	theme	essential	1093:1101	arg1	information					1103:1113	essential information	1093:1113	essential information	1093:1113	This study provides a rapid extraction technology and essential information for the production of DDFPs, which could be potentially used as healthcare food.
29581723	1	36	theme	surface	132:138	arg1	methodology					140:150	Response surface methodology	123:150	Response surface methodology (RSM)	123:156	Response surface methodology (RSM) was employed to optimize the conditions for the ultrasonic-assisted extraction (UAE) of polysaccharides from the flowers of Dendrobium devonianum.
29581723	1	36	theme	surface	132:138	arg1	RSM					153:155	RSM	153:155	RSM	153:155	Response surface methodology (RSM) was employed to optimize the conditions for the ultrasonic-assisted extraction (UAE) of polysaccharides from the flowers of Dendrobium devonianum.
29581723	7	37	theme	DDFPs	1137:1141	arg1	production					1123:1132	the production	1119:1132	the production of DDFPs	1119:1141	This study provides a rapid extraction technology and essential information for the production of DDFPs, which could be potentially used as healthcare food.
29581723	3	38	theme	precipitation	654:666	arg1	method					668:673	the stepwise ethanol precipitation method	633:673	the stepwise ethanol precipitation method	633:673	Furthermore, three fractions (DDFPs30, DDFPs50, and DDFPs70) were prepared from Dendrobium devonianum flowers polysaccharides (DDFPs) by the stepwise ethanol precipitation method.
29581723	3	39	theme	flowers	598:604	arg1	DDFPs					623:627	Dendrobium devonianum flowers polysaccharides (DDFPs)	576:628	Dendrobium devonianum flowers polysaccharides (DDFPs)	576:628	Furthermore, three fractions (DDFPs30, DDFPs50, and DDFPs70) were prepared from Dendrobium devonianum flowers polysaccharides (DDFPs) by the stepwise ethanol precipitation method.
29581723	2	40	theme	maximum	336:342	arg1	yields					344:349	the maximum yields	332:349	the maximum yields of DDFPs	332:358	The optimal conditions for the maximum yields of DDFPs are as follows: an extraction temperature of 63.13°C, an extraction time of 53.10 min, and a water-to-raw material ratio of 22.11 mL/g.
29581723	6	41	theme	D.	1018:1019	arg1	plant					1032:1036	the D. officinale plant	1014:1036	the D. officinale plant	1014:1036	In particular, the monosaccharide composition analysis of the DDFPs indicates that mannose and glucose are the primary components, similar to those of the D. officinale plant.
29581723	3	42	theme	polysaccharides	606:620	arg1	DDFPs					623:627	Dendrobium devonianum flowers polysaccharides (DDFPs)	576:628	Dendrobium devonianum flowers polysaccharides (DDFPs)	576:628	Furthermore, three fractions (DDFPs30, DDFPs50, and DDFPs70) were prepared from Dendrobium devonianum flowers polysaccharides (DDFPs) by the stepwise ethanol precipitation method.
29581723	4	43	theme	highest	702:708	arg1	activity					722:729	the highest antioxidant activity	698:729	the highest antioxidant activity	698:729	The DDFPs50 exhibited the highest antioxidant activity compared to the other fractions.
29581723	4	44	theme	antioxidant	710:720	arg1	activity					722:729	the highest antioxidant activity	698:729	the highest antioxidant activity	698:729	The DDFPs50 exhibited the highest antioxidant activity compared to the other fractions.
29581723	2	45	theme	22.11 mL/g	484:493	arg1	ratio					475:479	a water-to-raw material ratio	451:479	a water-to-raw material ratio of 22.11 mL/g	451:493	The optimal conditions for the maximum yields of DDFPs are as follows: an extraction temperature of 63.13°C, an extraction time of 53.10 min, and a water-to-raw material ratio of 22.11 mL/g.
29581723	3	46	theme	stepwise	637:644	arg1	method					668:673	the stepwise ethanol precipitation method	633:673	the stepwise ethanol precipitation method	633:673	Furthermore, three fractions (DDFPs30, DDFPs50, and DDFPs70) were prepared from Dendrobium devonianum flowers polysaccharides (DDFPs) by the stepwise ethanol precipitation method.
29581723	3	47	theme	Dendrobium	576:585	arg1	DDFPs					623:627	Dendrobium devonianum flowers polysaccharides (DDFPs)	576:628	Dendrobium devonianum flowers polysaccharides (DDFPs)	576:628	Furthermore, three fractions (DDFPs30, DDFPs50, and DDFPs70) were prepared from Dendrobium devonianum flowers polysaccharides (DDFPs) by the stepwise ethanol precipitation method.
29581723	6	48	theme	DDFPs	925:929	arg1	analysis					909:916	the monosaccharide composition analysis	878:916	the monosaccharide composition analysis of the DDFPs	878:929	In particular, the monosaccharide composition analysis of the DDFPs indicates that mannose and glucose are the primary components, similar to those of the D. officinale plant.
29581723	3	49	theme	devonianum	587:596	arg1	DDFPs					623:627	Dendrobium devonianum flowers polysaccharides (DDFPs)	576:628	Dendrobium devonianum flowers polysaccharides (DDFPs)	576:628	Furthermore, three fractions (DDFPs30, DDFPs50, and DDFPs70) were prepared from Dendrobium devonianum flowers polysaccharides (DDFPs) by the stepwise ethanol precipitation method.
29616894	13	0	theme	=JCM	1982:1985	arg1	33880T					2011:2016	=JCM 32184T=MTCC 12637T=KCTC 33880T	1982:2016	=JCM 32184T=MTCC 12637T=KCTC 33880T	1982:2016	The type strain is AK73T (=JCM 32184T=MTCC 12637T=KCTC 33880T).
29616894	13	0	theme	=JCM	1982:1985	arg1	AK73T					1975:1979	AK73T	1975:1979	AK73T (=JCM 32184T=MTCC 12637T=KCTC 33880T)	1975:2017	The type strain is AK73T (=JCM 32184T=MTCC 12637T=KCTC 33880T).
29616894	3	1	from	pH	443:444	arg1	agar					435:438	marine agar	428:438	marine agar at pH 9	428:446	Colonies were circular, 1-2 mm in diameter, glossy, smooth, yellowish and convex with an entire margin after 48 h growth on marine agar at pH 9 and 37 °C. Growth occurred at 15-42 °C, 0-10 % (w/v) NaCl and at a pH range of 7-12.
29616894	10	2	theme	organisms	1607:1615	arg1	cluster					1596:1602	a peripheral cluster	1583:1602	a peripheral cluster of organisms that contained Bacillus gibsonii, Bacillus murimartini and Bacillus plakortidis with similarity values of 93.6, 93.5 and 93.4 %, respectively	1583:1757	Phylogenetic analysis showed that strain AK73T formed a separate lineage but was loosely associated with a peripheral cluster of organisms that contained Bacillus gibsonii, Bacillus murimartini and Bacillus plakortidis with similarity values of 93.6, 93.5 and 93.4 %, respectively.
29616894	13	3	theme	12637T=KCTC	1999:2009	arg1	33880T					2011:2016	=JCM 32184T=MTCC 12637T=KCTC 33880T	1982:2016	=JCM 32184T=MTCC 12637T=KCTC 33880T	1982:2016	The type strain is AK73T (=JCM 32184T=MTCC 12637T=KCTC 33880T).
29616894	13	3	theme	12637T=KCTC	1999:2009	arg1	AK73T					1975:1979	AK73T	1975:1979	AK73T (=JCM 32184T=MTCC 12637T=KCTC 33880T)	1975:2017	The type strain is AK73T (=JCM 32184T=MTCC 12637T=KCTC 33880T).
29616894	4	4	theme	Strain	533:538	arg1	AK73T					540:544	Strain AK73T	533:544	Strain AK73T	533:544	Strain AK73T was positive for catalase and arginine dihydrolase 2 activities, hydrolysis of Tweens 20, 40 and 80, and negative for esculinase, caseinase, gelatinase, β-galactosidase, lipase (Tween 60) and urease activities.
29616894	3	5	theme	37 °C.	452:457	arg1	Growth					459:464	37 °C. Growth	452:464	37 °C. Growth	452:464	Colonies were circular, 1-2 mm in diameter, glossy, smooth, yellowish and convex with an entire margin after 48 h growth on marine agar at pH 9 and 37 °C. Growth occurred at 15-42 °C, 0-10 % (w/v) NaCl and at a pH range of 7-12.
29616894	6	6	theme	diamino	1035:1041	arg1	acid					1043:1046	the diagnostic diamino acid	1020:1046	the diagnostic diamino acid	1020:1046	The cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid.
29616894	6	6	theme	diamino	1035:1041	arg1	acid					1012:1015	meso-diaminopimelic acid	992:1015	meso-diaminopimelic acid	992:1015	The cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid.
29616894	3	7	theme	0-10 	488:492	arg1	NaCl					501:504	15-42 °C, 0-10 % (w/v) NaCl	478:504	NaCl	501:504	Colonies were circular, 1-2 mm in diameter, glossy, smooth, yellowish and convex with an entire margin after 48 h growth on marine agar at pH 9 and 37 °C. Growth occurred at 15-42 °C, 0-10 % (w/v) NaCl and at a pH range of 7-12.
29616894	2	8	theme	strain	199:204	arg1	bacterium					177:185	An aerobic, endospore-forming, haloalkali-tolerant, Gram-stain-positive, motile, rod-shaped bacterium	85:185	bacterium	177:185	An aerobic, endospore-forming, haloalkali-tolerant, Gram-stain-positive, motile, rod-shaped bacterium, designated strain AK73T, was isolated from a sediment sample collected from Sambhar lake, Jaipur, Rajasthan, India.
29616894	2	8	theme	strain	199:204	arg1	AK73T					206:210	designated strain AK73T	188:210	designated strain AK73T	188:210	An aerobic, endospore-forming, haloalkali-tolerant, Gram-stain-positive, motile, rod-shaped bacterium, designated strain AK73T, was isolated from a sediment sample collected from Sambhar lake, Jaipur, Rajasthan, India.
29616894	13	9	theme	type	1960:1963	arg1	strain					1965:1970	The type strain	1956:1970	The type strain	1956:1970	The type strain is AK73T (=JCM 32184T=MTCC 12637T=KCTC 33880T).
29616894	13	9	theme	type	1960:1963	arg1	AK73T					1975:1979	AK73T	1975:1979	AK73T (=JCM 32184T=MTCC 12637T=KCTC 33880T)	1975:2017	The type strain is AK73T (=JCM 32184T=MTCC 12637T=KCTC 33880T).
29616894	11	10	theme	Bacillus	1884:1891	arg1	species					1863:1869	a novel species	1855:1869	a novel species	1855:1869	Based on its phenotypic characteristics and on phylogenetic inference, strain AK73T represents a novel species of the genus Bacillus, for which the name Bacillus alkalilacus sp.
29616894	8	11	theme	54 mol	1282:1287	arg1	G+C content					1250:1260	The DNA G+C content	1242:1260	The DNA G+C content of strain AK73T	1242:1276	The DNA G+C content of strain AK73T was 54 mol%.
29616894	8	11	theme	54 mol	1282:1287	arg1	%					1288:1288	54 mol%	1282:1288	54 mol%	1282:1288	The DNA G+C content of strain AK73T was 54 mol%.
29616894	7	12	theme	unidentified	1221:1232	arg1	diphosphatidylglycerol					1071:1092	diphosphatidylglycerol	1071:1092	diphosphatidylglycerol	1071:1092	The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, one unidentified aminophospholipid, four unidentified phospholipids and three unidentified lipids.
29616894	7	12	theme	unidentified	1221:1232	arg1	lipids					1234:1239	three unidentified lipids	1215:1239	three unidentified lipids	1215:1239	The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, one unidentified aminophospholipid, four unidentified phospholipids and three unidentified lipids.
29616894	3	13	from	circular	318:325	arg1	diameter					338:345	diameter	338:345	diameter	338:345	Colonies were circular, 1-2 mm in diameter, glossy, smooth, yellowish and convex with an entire margin after 48 h growth on marine agar at pH 9 and 37 °C. Growth occurred at 15-42 °C, 0-10 % (w/v) NaCl and at a pH range of 7-12.
29616894	3	13	from	circular	318:325	arg1	convex					378:383	convex	378:383	convex	378:383	Colonies were circular, 1-2 mm in diameter, glossy, smooth, yellowish and convex with an entire margin after 48 h growth on marine agar at pH 9 and 37 °C. Growth occurred at 15-42 °C, 0-10 % (w/v) NaCl and at a pH range of 7-12.
29616894	3	13	from	circular	318:325	arg1	yellowish					364:372	yellowish	364:372	yellowish	364:372	Colonies were circular, 1-2 mm in diameter, glossy, smooth, yellowish and convex with an entire margin after 48 h growth on marine agar at pH 9 and 37 °C. Growth occurred at 15-42 °C, 0-10 % (w/v) NaCl and at a pH range of 7-12.
29616894	3	13	from	circular	318:325	arg1	smooth					356:361	smooth	356:361	smooth	356:361	Colonies were circular, 1-2 mm in diameter, glossy, smooth, yellowish and convex with an entire margin after 48 h growth on marine agar at pH 9 and 37 °C. Growth occurred at 15-42 °C, 0-10 % (w/v) NaCl and at a pH range of 7-12.
29616894	3	13	from	circular	318:325	arg1	glossy					348:353	glossy	348:353	glossy	348:353	Colonies were circular, 1-2 mm in diameter, glossy, smooth, yellowish and convex with an entire margin after 48 h growth on marine agar at pH 9 and 37 °C. Growth occurred at 15-42 °C, 0-10 % (w/v) NaCl and at a pH range of 7-12.
29616894	9	14	theme	16S	1321:1323	arg1	analysis					1344:1351	comparative 16S rRNA gene sequence analysis	1309:1351	comparative 16S rRNA gene sequence analysis	1309:1351	Analysis based on comparative 16S rRNA gene sequence analysis indicated that Bacillus alcalophilus was the nearest phylogenetic neighbour, with a pair-wise sequence similarity of 96.0 %.
29616894	3	15	with	circular	318:325	arg1	margin					400:405	an entire margin	390:405	an entire margin after 48 h growth on marine agar at pH 9	390:446	Colonies were circular, 1-2 mm in diameter, glossy, smooth, yellowish and convex with an entire margin after 48 h growth on marine agar at pH 9 and 37 °C. Growth occurred at 15-42 °C, 0-10 % (w/v) NaCl and at a pH range of 7-12.
29616894	11	16	theme	alkalilacus	1922:1932	arg1	sp					1934:1935	Bacillus alkalilacus sp	1913:1935	the name Bacillus alkalilacus sp	1904:1935	Based on its phenotypic characteristics and on phylogenetic inference, strain AK73T represents a novel species of the genus Bacillus, for which the name Bacillus alkalilacus sp.
29616894	4	17	theme	dihydrolase	585:595	arg1	activities					599:608	catalase and arginine dihydrolase 2 activities	563:608	catalase and arginine dihydrolase 2 activities	563:608	Strain AK73T was positive for catalase and arginine dihydrolase 2 activities, hydrolysis of Tweens 20, 40 and 80, and negative for esculinase, caseinase, gelatinase, β-galactosidase, lipase (Tween 60) and urease activities.
29616894	11	18	theme	phylogenetic	1807:1818	arg1	inference					1820:1828	phylogenetic inference	1807:1828	phylogenetic inference	1807:1828	Based on its phenotypic characteristics and on phylogenetic inference, strain AK73T represents a novel species of the genus Bacillus, for which the name Bacillus alkalilacus sp.
29616894	9	19	theme	gene	1330:1333	arg1	analysis					1344:1351	comparative 16S rRNA gene sequence analysis	1309:1351	comparative 16S rRNA gene sequence analysis	1309:1351	Analysis based on comparative 16S rRNA gene sequence analysis indicated that Bacillus alcalophilus was the nearest phylogenetic neighbour, with a pair-wise sequence similarity of 96.0 %.
29616894	4	20	theme	urease	738:743	arg1	activities					745:754	urease activities	738:754	urease activities	738:754	Strain AK73T was positive for catalase and arginine dihydrolase 2 activities, hydrolysis of Tweens 20, 40 and 80, and negative for esculinase, caseinase, gelatinase, β-galactosidase, lipase (Tween 60) and urease activities.
29616894	6	21	theme	meso-diaminopimelic	992:1010	arg1	acid					1043:1046	the diagnostic diamino acid	1020:1046	the diagnostic diamino acid	1020:1046	The cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid.
29616894	6	21	theme	meso-diaminopimelic	992:1010	arg1	acid					1012:1015	meso-diaminopimelic acid	992:1015	meso-diaminopimelic acid	992:1015	The cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid.
29616894	7	22	theme	unidentified	1184:1195	arg1	phospholipids					1197:1209	four unidentified phospholipids	1179:1209	four unidentified phospholipids	1179:1209	The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, one unidentified aminophospholipid, four unidentified phospholipids and three unidentified lipids.
29616894	7	22	theme	unidentified	1184:1195	arg1	diphosphatidylglycerol					1071:1092	diphosphatidylglycerol	1071:1092	diphosphatidylglycerol	1071:1092	The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, one unidentified aminophospholipid, four unidentified phospholipids and three unidentified lipids.
29616894	10	23	theme	similarity	1702:1711	arg1	values					1713:1718	similarity values	1702:1718	similarity values of 93.6, 93.5 and 93.4 %, respectively	1702:1757	Phylogenetic analysis showed that strain AK73T formed a separate lineage but was loosely associated with a peripheral cluster of organisms that contained Bacillus gibsonii, Bacillus murimartini and Bacillus plakortidis with similarity values of 93.6, 93.5 and 93.4 %, respectively.
29616894	5	24	theme	fatty	761:765	arg1	acids					767:771	The fatty acids	757:771	The fatty acids	757:771	The fatty acids were dominated by branched iso-, anteiso-, saturated fatty acids with a high abundance of iso-C15 : 0, anteiso-C15 : 0, C16 : 0 and anteiso-C17 : 0; MK-7 was the major menaquinone.
29616894	3	25	from	glossy	348:353	arg1	circular					318:325	circular	318:325	circular	318:325	Colonies were circular, 1-2 mm in diameter, glossy, smooth, yellowish and convex with an entire margin after 48 h growth on marine agar at pH 9 and 37 °C. Growth occurred at 15-42 °C, 0-10 % (w/v) NaCl and at a pH range of 7-12.
29616894	3	26	from	smooth	356:361	arg1	circular					318:325	circular	318:325	circular	318:325	Colonies were circular, 1-2 mm in diameter, glossy, smooth, yellowish and convex with an entire margin after 48 h growth on marine agar at pH 9 and 37 °C. Growth occurred at 15-42 °C, 0-10 % (w/v) NaCl and at a pH range of 7-12.
29616894	10	27	contain	contained	1622:1630	arg1	organisms					1607:1615	organisms	1607:1615	organisms that contained Bacillus gibsonii, Bacillus murimartini and Bacillus plakortidis with similarity values of 93.6, 93.5 and 93.4 %, respectively	1607:1757	Phylogenetic analysis showed that strain AK73T formed a separate lineage but was loosely associated with a peripheral cluster of organisms that contained Bacillus gibsonii, Bacillus murimartini and Bacillus plakortidis with similarity values of 93.6, 93.5 and 93.4 %, respectively.
29616894	10	27	contain	contained	1622:1630	arg2	gibsonii					1641:1648	Bacillus gibsonii	1632:1648	Bacillus gibsonii	1632:1648	Phylogenetic analysis showed that strain AK73T formed a separate lineage but was loosely associated with a peripheral cluster of organisms that contained Bacillus gibsonii, Bacillus murimartini and Bacillus plakortidis with similarity values of 93.6, 93.5 and 93.4 %, respectively.
29616894	10	27	contain	contained	1622:1630	arg2	plakortidis					1685:1695	Bacillus plakortidis	1676:1695	Bacillus plakortidis	1676:1695	Phylogenetic analysis showed that strain AK73T formed a separate lineage but was loosely associated with a peripheral cluster of organisms that contained Bacillus gibsonii, Bacillus murimartini and Bacillus plakortidis with similarity values of 93.6, 93.5 and 93.4 %, respectively.
29616894	10	27	contain	contained	1622:1630	arg2	murimartini					1660:1670	Bacillus murimartini	1651:1670	Bacillus murimartini	1651:1670	Phylogenetic analysis showed that strain AK73T formed a separate lineage but was loosely associated with a peripheral cluster of organisms that contained Bacillus gibsonii, Bacillus murimartini and Bacillus plakortidis with similarity values of 93.6, 93.5 and 93.4 %, respectively.
29616894	1	28	theme	sediment	47:54	arg1	sample					56:61	a sediment sample	45:61	a sediment sample from a lake in India	45:82	nov., isolated from a sediment sample from a lake in India.
29616894	10	29	theme	strain	1512:1517	arg1	AK73T					1519:1523	strain AK73T	1512:1523	strain AK73T	1512:1523	Phylogenetic analysis showed that strain AK73T formed a separate lineage but was loosely associated with a peripheral cluster of organisms that contained Bacillus gibsonii, Bacillus murimartini and Bacillus plakortidis with similarity values of 93.6, 93.5 and 93.4 %, respectively.
29616894	3	30	from	diameter	338:345	arg1	circular					318:325	circular	318:325	circular	318:325	Colonies were circular, 1-2 mm in diameter, glossy, smooth, yellowish and convex with an entire margin after 48 h growth on marine agar at pH 9 and 37 °C. Growth occurred at 15-42 °C, 0-10 % (w/v) NaCl and at a pH range of 7-12.
29616894	9	31	theme	phylogenetic	1406:1417	arg1	neighbour					1419:1427	the nearest phylogenetic neighbour	1394:1427	the nearest phylogenetic neighbour	1394:1427	Analysis based on comparative 16S rRNA gene sequence analysis indicated that Bacillus alcalophilus was the nearest phylogenetic neighbour, with a pair-wise sequence similarity of 96.0 %.
29616894	9	31	theme	phylogenetic	1406:1417	arg1	alcalophilus					1377:1388	Bacillus alcalophilus	1368:1388	Bacillus alcalophilus	1368:1388	Analysis based on comparative 16S rRNA gene sequence analysis indicated that Bacillus alcalophilus was the nearest phylogenetic neighbour, with a pair-wise sequence similarity of 96.0 %.
29616894	0	32	theme	Bacillus	0:7	arg1	sp					21:22	Bacillus alkalilacus sp	0:22	Bacillus alkalilacus sp.	0:23	Bacillus alkalilacus sp.
29616894	5	33	theme	major	935:939	arg1	menaquinone					941:951	the major menaquinone	931:951	the major menaquinone	931:951	The fatty acids were dominated by branched iso-, anteiso-, saturated fatty acids with a high abundance of iso-C15 : 0, anteiso-C15 : 0, C16 : 0 and anteiso-C17 : 0; MK-7 was the major menaquinone.
29616894	5	33	theme	major	935:939	arg1	 0					872:873	 0	872:873	 0	872:873	The fatty acids were dominated by branched iso-, anteiso-, saturated fatty acids with a high abundance of iso-C15 : 0, anteiso-C15 : 0, C16 : 0 and anteiso-C17 : 0; MK-7 was the major menaquinone.
29616894	2	34	dep	endospore-forming	97:113	arg1	motile					158:163	motile	158:163	motile	158:163	An aerobic, endospore-forming, haloalkali-tolerant, Gram-stain-positive, motile, rod-shaped bacterium, designated strain AK73T, was isolated from a sediment sample collected from Sambhar lake, Jaipur, Rajasthan, India.
29616894	2	34	dep	endospore-forming	97:113	arg1	Gram-stain-positive					137:155	Gram-stain-positive	137:155	Gram-stain-positive	137:155	An aerobic, endospore-forming, haloalkali-tolerant, Gram-stain-positive, motile, rod-shaped bacterium, designated strain AK73T, was isolated from a sediment sample collected from Sambhar lake, Jaipur, Rajasthan, India.
29616894	2	34	dep	endospore-forming	97:113	arg1	haloalkali-tolerant					116:134	haloalkali-tolerant	116:134	haloalkali-tolerant	116:134	An aerobic, endospore-forming, haloalkali-tolerant, Gram-stain-positive, motile, rod-shaped bacterium, designated strain AK73T, was isolated from a sediment sample collected from Sambhar lake, Jaipur, Rajasthan, India.
29616894	4	35	theme	Tweens	625:630	arg1	hydrolysis					611:620	hydrolysis	611:620	hydrolysis of Tweens 20, 40 and 80	611:644	Strain AK73T was positive for catalase and arginine dihydrolase 2 activities, hydrolysis of Tweens 20, 40 and 80, and negative for esculinase, caseinase, gelatinase, β-galactosidase, lipase (Tween 60) and urease activities.
29616894	4	35	theme	Tweens	625:630	arg1	activities					599:608	catalase and arginine dihydrolase 2 activities	563:608	catalase and arginine dihydrolase 2 activities	563:608	Strain AK73T was positive for catalase and arginine dihydrolase 2 activities, hydrolysis of Tweens 20, 40 and 80, and negative for esculinase, caseinase, gelatinase, β-galactosidase, lipase (Tween 60) and urease activities.
29616894	10	36	theme	separate	1534:1541	arg1	lineage					1543:1549	a separate lineage	1532:1549	a separate lineage	1532:1549	Phylogenetic analysis showed that strain AK73T formed a separate lineage but was loosely associated with a peripheral cluster of organisms that contained Bacillus gibsonii, Bacillus murimartini and Bacillus plakortidis with similarity values of 93.6, 93.5 and 93.4 %, respectively.
29616894	3	37	theme	7-12	527:530	arg1	range					518:522	a pH range	513:522	a pH range of 7-12	513:530	Colonies were circular, 1-2 mm in diameter, glossy, smooth, yellowish and convex with an entire margin after 48 h growth on marine agar at pH 9 and 37 °C. Growth occurred at 15-42 °C, 0-10 % (w/v) NaCl and at a pH range of 7-12.
29616894	9	38	theme	sequence	1447:1454	arg1	similarity					1456:1465	a pair-wise sequence similarity	1435:1465	a pair-wise sequence similarity of 96.0 %	1435:1475	Analysis based on comparative 16S rRNA gene sequence analysis indicated that Bacillus alcalophilus was the nearest phylogenetic neighbour, with a pair-wise sequence similarity of 96.0 %.
29616894	3	39	theme	pH	515:516	arg1	range					518:522	a pH range	513:522	a pH range of 7-12	513:530	Colonies were circular, 1-2 mm in diameter, glossy, smooth, yellowish and convex with an entire margin after 48 h growth on marine agar at pH 9 and 37 °C. Growth occurred at 15-42 °C, 0-10 % (w/v) NaCl and at a pH range of 7-12.
29616894	3	40	dep	circular	318:325	arg1	1-2 mm					328:333	1-2 mm	328:333	1-2 mm	328:333	Colonies were circular, 1-2 mm in diameter, glossy, smooth, yellowish and convex with an entire margin after 48 h growth on marine agar at pH 9 and 37 °C. Growth occurred at 15-42 °C, 0-10 % (w/v) NaCl and at a pH range of 7-12.
29616894	10	41	theme	%	1743:1743	arg1	values					1713:1718	similarity values	1702:1718	similarity values of 93.6, 93.5 and 93.4 %, respectively	1702:1757	Phylogenetic analysis showed that strain AK73T formed a separate lineage but was loosely associated with a peripheral cluster of organisms that contained Bacillus gibsonii, Bacillus murimartini and Bacillus plakortidis with similarity values of 93.6, 93.5 and 93.4 %, respectively.
29616894	8	42	theme	strain	1265:1270	arg1	AK73T					1272:1276	strain AK73T	1265:1276	strain AK73T	1265:1276	The DNA G+C content of strain AK73T was 54 mol%.
29616894	3	43	theme	48 h	413:416	arg1	growth					418:423	48 h growth	413:423	48 h growth on marine agar at pH 9	413:446	Colonies were circular, 1-2 mm in diameter, glossy, smooth, yellowish and convex with an entire margin after 48 h growth on marine agar at pH 9 and 37 °C. Growth occurred at 15-42 °C, 0-10 % (w/v) NaCl and at a pH range of 7-12.
29616894	9	44	theme	%	1475:1475	arg1	similarity					1456:1465	a pair-wise sequence similarity	1435:1465	a pair-wise sequence similarity of 96.0 %	1435:1475	Analysis based on comparative 16S rRNA gene sequence analysis indicated that Bacillus alcalophilus was the nearest phylogenetic neighbour, with a pair-wise sequence similarity of 96.0 %.
29616894	3	45	theme	entire	393:398	arg1	margin					400:405	an entire margin	390:405	an entire margin after 48 h growth on marine agar at pH 9	390:446	Colonies were circular, 1-2 mm in diameter, glossy, smooth, yellowish and convex with an entire margin after 48 h growth on marine agar at pH 9 and 37 °C. Growth occurred at 15-42 °C, 0-10 % (w/v) NaCl and at a pH range of 7-12.
29616894	10	46	theme	peripheral	1585:1594	arg1	cluster					1596:1602	a peripheral cluster	1583:1602	a peripheral cluster of organisms that contained Bacillus gibsonii, Bacillus murimartini and Bacillus plakortidis with similarity values of 93.6, 93.5 and 93.4 %, respectively	1583:1757	Phylogenetic analysis showed that strain AK73T formed a separate lineage but was loosely associated with a peripheral cluster of organisms that contained Bacillus gibsonii, Bacillus murimartini and Bacillus plakortidis with similarity values of 93.6, 93.5 and 93.4 %, respectively.
29616894	1	47	from	lake	70:73	arg1	sample					56:61	a sediment sample	45:61	a sediment sample from a lake in India	45:82	nov., isolated from a sediment sample from a lake in India.
29616894	1	47	from	lake	70:73	arg1	India					78:82	India	78:82	India	78:82	nov., isolated from a sediment sample from a lake in India.
29616894	11	48	dep	name	1908:1911	arg1	sp					1934:1935	Bacillus alkalilacus sp	1913:1935	the name Bacillus alkalilacus sp	1904:1935	Based on its phenotypic characteristics and on phylogenetic inference, strain AK73T represents a novel species of the genus Bacillus, for which the name Bacillus alkalilacus sp.
29616894	2	49	theme	aerobic	88:94	arg1	bacterium					177:185	An aerobic, endospore-forming, haloalkali-tolerant, Gram-stain-positive, motile, rod-shaped bacterium	85:185	bacterium	177:185	An aerobic, endospore-forming, haloalkali-tolerant, Gram-stain-positive, motile, rod-shaped bacterium, designated strain AK73T, was isolated from a sediment sample collected from Sambhar lake, Jaipur, Rajasthan, India.
29616894	2	49	theme	aerobic	88:94	arg1	AK73T					206:210	designated strain AK73T	188:210	designated strain AK73T	188:210	An aerobic, endospore-forming, haloalkali-tolerant, Gram-stain-positive, motile, rod-shaped bacterium, designated strain AK73T, was isolated from a sediment sample collected from Sambhar lake, Jaipur, Rajasthan, India.
29616894	5	50	dep	 0	872:873	arg1	anteiso-C17 					905:916	anteiso-C17 	905:916	anteiso-C17 	905:916	The fatty acids were dominated by branched iso-, anteiso-, saturated fatty acids with a high abundance of iso-C15 : 0, anteiso-C15 : 0, C16 : 0 and anteiso-C17 : 0; MK-7 was the major menaquinone.
29616894	5	50	dep	 0	872:873	arg1	 0					918:919	 0	918:919	 0	918:919	The fatty acids were dominated by branched iso-, anteiso-, saturated fatty acids with a high abundance of iso-C15 : 0, anteiso-C15 : 0, C16 : 0 and anteiso-C17 : 0; MK-7 was the major menaquinone.
29616894	5	50	dep	 0	872:873	arg1	C16 					893:896	C16 	893:896	C16 	893:896	The fatty acids were dominated by branched iso-, anteiso-, saturated fatty acids with a high abundance of iso-C15 : 0, anteiso-C15 : 0, C16 : 0 and anteiso-C17 : 0; MK-7 was the major menaquinone.
29616894	5	50	dep	 0	872:873	arg1	 0					898:899	 0	898:899	 0	898:899	The fatty acids were dominated by branched iso-, anteiso-, saturated fatty acids with a high abundance of iso-C15 : 0, anteiso-C15 : 0, C16 : 0 and anteiso-C17 : 0; MK-7 was the major menaquinone.
29616894	5	50	dep	 0	872:873	arg1	MK-7					922:925	MK-7	922:925	MK-7	922:925	The fatty acids were dominated by branched iso-, anteiso-, saturated fatty acids with a high abundance of iso-C15 : 0, anteiso-C15 : 0, C16 : 0 and anteiso-C17 : 0; MK-7 was the major menaquinone.
29616894	5	50	dep	 0	872:873	arg1	 0					889:890	 0	889:890	 0	889:890	The fatty acids were dominated by branched iso-, anteiso-, saturated fatty acids with a high abundance of iso-C15 : 0, anteiso-C15 : 0, C16 : 0 and anteiso-C17 : 0; MK-7 was the major menaquinone.
29616894	13	51	theme	32184T=MTCC	1987:1997	arg1	33880T					2011:2016	=JCM 32184T=MTCC 12637T=KCTC 33880T	1982:2016	=JCM 32184T=MTCC 12637T=KCTC 33880T	1982:2016	The type strain is AK73T (=JCM 32184T=MTCC 12637T=KCTC 33880T).
29616894	13	51	theme	32184T=MTCC	1987:1997	arg1	AK73T					1975:1979	AK73T	1975:1979	AK73T (=JCM 32184T=MTCC 12637T=KCTC 33880T)	1975:2017	The type strain is AK73T (=JCM 32184T=MTCC 12637T=KCTC 33880T).
29616894	11	52	theme	novel	1857:1861	arg1	species					1863:1869	a novel species	1855:1869	a novel species	1855:1869	Based on its phenotypic characteristics and on phylogenetic inference, strain AK73T represents a novel species of the genus Bacillus, for which the name Bacillus alkalilacus sp.
29616894	2	53	theme	endospore-forming	97:113	arg1	bacterium					177:185	An aerobic, endospore-forming, haloalkali-tolerant, Gram-stain-positive, motile, rod-shaped bacterium	85:185	bacterium	177:185	An aerobic, endospore-forming, haloalkali-tolerant, Gram-stain-positive, motile, rod-shaped bacterium, designated strain AK73T, was isolated from a sediment sample collected from Sambhar lake, Jaipur, Rajasthan, India.
29616894	2	53	theme	endospore-forming	97:113	arg1	AK73T					206:210	designated strain AK73T	188:210	designated strain AK73T	188:210	An aerobic, endospore-forming, haloalkali-tolerant, Gram-stain-positive, motile, rod-shaped bacterium, designated strain AK73T, was isolated from a sediment sample collected from Sambhar lake, Jaipur, Rajasthan, India.
29616894	3	54	from	growth	418:423	arg1	agar					435:438	marine agar	428:438	marine agar at pH 9	428:446	Colonies were circular, 1-2 mm in diameter, glossy, smooth, yellowish and convex with an entire margin after 48 h growth on marine agar at pH 9 and 37 °C. Growth occurred at 15-42 °C, 0-10 % (w/v) NaCl and at a pH range of 7-12.
29616894	9	55	with	neighbour	1419:1427	arg1	similarity					1456:1465	a pair-wise sequence similarity	1435:1465	a pair-wise sequence similarity of 96.0 %	1435:1475	Analysis based on comparative 16S rRNA gene sequence analysis indicated that Bacillus alcalophilus was the nearest phylogenetic neighbour, with a pair-wise sequence similarity of 96.0 %.
29616894	11	56	theme	Bacillus	1913:1920	arg1	sp					1934:1935	Bacillus alkalilacus sp	1913:1935	the name Bacillus alkalilacus sp	1904:1935	Based on its phenotypic characteristics and on phylogenetic inference, strain AK73T represents a novel species of the genus Bacillus, for which the name Bacillus alkalilacus sp.
29616894	10	57	theme	Phylogenetic	1478:1489	arg1	analysis					1491:1498	Phylogenetic analysis	1478:1498	Phylogenetic analysis	1478:1498	Phylogenetic analysis showed that strain AK73T formed a separate lineage but was loosely associated with a peripheral cluster of organisms that contained Bacillus gibsonii, Bacillus murimartini and Bacillus plakortidis with similarity values of 93.6, 93.5 and 93.4 %, respectively.
29616894	3	58	theme	w/v	496:498	arg1	NaCl					501:504	15-42 °C, 0-10 % (w/v) NaCl	478:504	NaCl	501:504	Colonies were circular, 1-2 mm in diameter, glossy, smooth, yellowish and convex with an entire margin after 48 h growth on marine agar at pH 9 and 37 °C. Growth occurred at 15-42 °C, 0-10 % (w/v) NaCl and at a pH range of 7-12.
29616894	9	59	theme	comparative	1309:1319	arg1	analysis					1344:1351	comparative 16S rRNA gene sequence analysis	1309:1351	comparative 16S rRNA gene sequence analysis	1309:1351	Analysis based on comparative 16S rRNA gene sequence analysis indicated that Bacillus alcalophilus was the nearest phylogenetic neighbour, with a pair-wise sequence similarity of 96.0 %.
29616894	3	60	theme	%	493:493	arg1	NaCl					501:504	15-42 °C, 0-10 % (w/v) NaCl	478:504	NaCl	501:504	Colonies were circular, 1-2 mm in diameter, glossy, smooth, yellowish and convex with an entire margin after 48 h growth on marine agar at pH 9 and 37 °C. Growth occurred at 15-42 °C, 0-10 % (w/v) NaCl and at a pH range of 7-12.
29616894	9	61	theme	rRNA	1325:1328	arg1	analysis					1344:1351	comparative 16S rRNA gene sequence analysis	1309:1351	comparative 16S rRNA gene sequence analysis	1309:1351	Analysis based on comparative 16S rRNA gene sequence analysis indicated that Bacillus alcalophilus was the nearest phylogenetic neighbour, with a pair-wise sequence similarity of 96.0 %.
29616894	4	62	theme	arginine	576:583	arg1	dihydrolase					585:595	arginine dihydrolase 2	576:597	arginine dihydrolase 2	576:597	Strain AK73T was positive for catalase and arginine dihydrolase 2 activities, hydrolysis of Tweens 20, 40 and 80, and negative for esculinase, caseinase, gelatinase, β-galactosidase, lipase (Tween 60) and urease activities.
29616894	5	63	theme	iso-C15 	863:870	arg1	abundance					850:858	a high abundance	843:858	a high abundance of iso-C15 	843:870	The fatty acids were dominated by branched iso-, anteiso-, saturated fatty acids with a high abundance of iso-C15 : 0, anteiso-C15 : 0, C16 : 0 and anteiso-C17 : 0; MK-7 was the major menaquinone.
29616894	2	64	theme	sediment	233:240	arg1	sample					242:247	a sediment sample	231:247	a sediment sample collected from Sambhar lake, Jaipur, Rajasthan, India	231:301	An aerobic, endospore-forming, haloalkali-tolerant, Gram-stain-positive, motile, rod-shaped bacterium, designated strain AK73T, was isolated from a sediment sample collected from Sambhar lake, Jaipur, Rajasthan, India.
29616894	9	65	theme	sequence	1335:1342	arg1	analysis					1344:1351	comparative 16S rRNA gene sequence analysis	1309:1351	comparative 16S rRNA gene sequence analysis	1309:1351	Analysis based on comparative 16S rRNA gene sequence analysis indicated that Bacillus alcalophilus was the nearest phylogenetic neighbour, with a pair-wise sequence similarity of 96.0 %.
29616894	11	66	theme	strain	1831:1836	arg1	AK73T					1838:1842	strain AK73T	1831:1842	strain AK73T	1831:1842	Based on its phenotypic characteristics and on phylogenetic inference, strain AK73T represents a novel species of the genus Bacillus, for which the name Bacillus alkalilacus sp.
29616894	2	67	attach	isolated	217:224	arg2	bacterium					177:185	An aerobic, endospore-forming, haloalkali-tolerant, Gram-stain-positive, motile, rod-shaped bacterium	85:185	bacterium	177:185	An aerobic, endospore-forming, haloalkali-tolerant, Gram-stain-positive, motile, rod-shaped bacterium, designated strain AK73T, was isolated from a sediment sample collected from Sambhar lake, Jaipur, Rajasthan, India.
29616894	2	67	attach	isolated	217:224	arg1	sample					242:247	a sediment sample	231:247	a sediment sample collected from Sambhar lake, Jaipur, Rajasthan, India	231:301	An aerobic, endospore-forming, haloalkali-tolerant, Gram-stain-positive, motile, rod-shaped bacterium, designated strain AK73T, was isolated from a sediment sample collected from Sambhar lake, Jaipur, Rajasthan, India.
29616894	2	67	attach	isolated	217:224	arg2	AK73T					206:210	designated strain AK73T	188:210	designated strain AK73T	188:210	An aerobic, endospore-forming, haloalkali-tolerant, Gram-stain-positive, motile, rod-shaped bacterium, designated strain AK73T, was isolated from a sediment sample collected from Sambhar lake, Jaipur, Rajasthan, India.
29616894	6	68	theme	cell-wall	958:966	arg1	peptidoglycan					968:980	The cell-wall peptidoglycan	954:980	The cell-wall peptidoglycan	954:980	The cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid.
29616894	4	69	theme	catalase	563:570	arg1	activities					599:608	catalase and arginine dihydrolase 2 activities	563:608	catalase and arginine dihydrolase 2 activities	563:608	Strain AK73T was positive for catalase and arginine dihydrolase 2 activities, hydrolysis of Tweens 20, 40 and 80, and negative for esculinase, caseinase, gelatinase, β-galactosidase, lipase (Tween 60) and urease activities.
29616894	5	70	theme	saturated	816:824	arg1	iso-					800:803	branched iso-	791:803	branched iso-	791:803	The fatty acids were dominated by branched iso-, anteiso-, saturated fatty acids with a high abundance of iso-C15 : 0, anteiso-C15 : 0, C16 : 0 and anteiso-C17 : 0; MK-7 was the major menaquinone.
29616894	5	70	theme	saturated	816:824	arg1	acids					832:836	saturated fatty acids	816:836	saturated fatty acids with a high abundance of iso-C15 	816:870	The fatty acids were dominated by branched iso-, anteiso-, saturated fatty acids with a high abundance of iso-C15 : 0, anteiso-C15 : 0, C16 : 0 and anteiso-C17 : 0; MK-7 was the major menaquinone.
29616894	5	71	theme	branched	791:798	arg1	iso-					800:803	branched iso-	791:803	branched iso-	791:803	The fatty acids were dominated by branched iso-, anteiso-, saturated fatty acids with a high abundance of iso-C15 : 0, anteiso-C15 : 0, C16 : 0 and anteiso-C17 : 0; MK-7 was the major menaquinone.
29616894	5	71	theme	branched	791:798	arg1	acids					832:836	saturated fatty acids	816:836	saturated fatty acids with a high abundance of iso-C15 	816:870	The fatty acids were dominated by branched iso-, anteiso-, saturated fatty acids with a high abundance of iso-C15 : 0, anteiso-C15 : 0, C16 : 0 and anteiso-C17 : 0; MK-7 was the major menaquinone.
29616894	5	71	theme	branched	791:798	arg1	anteiso-					806:813	anteiso-	806:813	anteiso-	806:813	The fatty acids were dominated by branched iso-, anteiso-, saturated fatty acids with a high abundance of iso-C15 : 0, anteiso-C15 : 0, C16 : 0 and anteiso-C17 : 0; MK-7 was the major menaquinone.
29616894	3	72	theme	15-42 °C	478:485	arg1	NaCl					501:504	15-42 °C, 0-10 % (w/v) NaCl	478:504	NaCl	501:504	Colonies were circular, 1-2 mm in diameter, glossy, smooth, yellowish and convex with an entire margin after 48 h growth on marine agar at pH 9 and 37 °C. Growth occurred at 15-42 °C, 0-10 % (w/v) NaCl and at a pH range of 7-12.
29616894	9	73	theme	nearest	1398:1404	arg1	neighbour					1419:1427	the nearest phylogenetic neighbour	1394:1427	the nearest phylogenetic neighbour	1394:1427	Analysis based on comparative 16S rRNA gene sequence analysis indicated that Bacillus alcalophilus was the nearest phylogenetic neighbour, with a pair-wise sequence similarity of 96.0 %.
29616894	9	73	theme	nearest	1398:1404	arg1	alcalophilus					1377:1388	Bacillus alcalophilus	1368:1388	Bacillus alcalophilus	1368:1388	Analysis based on comparative 16S rRNA gene sequence analysis indicated that Bacillus alcalophilus was the nearest phylogenetic neighbour, with a pair-wise sequence similarity of 96.0 %.
29616894	0	74	theme	alkalilacus	9:19	arg1	sp					21:22	Bacillus alkalilacus sp	0:22	Bacillus alkalilacus sp.	0:23	Bacillus alkalilacus sp.
29616894	2	75	theme	rod-shaped	166:175	arg1	bacterium					177:185	An aerobic, endospore-forming, haloalkali-tolerant, Gram-stain-positive, motile, rod-shaped bacterium	85:185	bacterium	177:185	An aerobic, endospore-forming, haloalkali-tolerant, Gram-stain-positive, motile, rod-shaped bacterium, designated strain AK73T, was isolated from a sediment sample collected from Sambhar lake, Jaipur, Rajasthan, India.
29616894	2	75	theme	rod-shaped	166:175	arg1	AK73T					206:210	designated strain AK73T	188:210	designated strain AK73T	188:210	An aerobic, endospore-forming, haloalkali-tolerant, Gram-stain-positive, motile, rod-shaped bacterium, designated strain AK73T, was isolated from a sediment sample collected from Sambhar lake, Jaipur, Rajasthan, India.
29616894	6	76	theme	diagnostic	1024:1033	arg1	acid					1043:1046	the diagnostic diamino acid	1020:1046	the diagnostic diamino acid	1020:1046	The cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid.
29616894	6	76	theme	diagnostic	1024:1033	arg1	acid					1012:1015	meso-diaminopimelic acid	992:1015	meso-diaminopimelic acid	992:1015	The cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid.
29616894	9	77	theme	pair-wise	1437:1445	arg1	similarity					1456:1465	a pair-wise sequence similarity	1435:1465	a pair-wise sequence similarity of 96.0 %	1435:1475	Analysis based on comparative 16S rRNA gene sequence analysis indicated that Bacillus alcalophilus was the nearest phylogenetic neighbour, with a pair-wise sequence similarity of 96.0 %.
29616894	10	78	theme	93.6	1723:1726	arg1	%					1743:1743	93.6, 93.5 and 93.4 %	1723:1743	%	1743:1743	Phylogenetic analysis showed that strain AK73T formed a separate lineage but was loosely associated with a peripheral cluster of organisms that contained Bacillus gibsonii, Bacillus murimartini and Bacillus plakortidis with similarity values of 93.6, 93.5 and 93.4 %, respectively.
29616894	11	79	theme	phenotypic	1773:1782	arg1	characteristics					1784:1798	its phenotypic characteristics	1769:1798	its phenotypic characteristics	1769:1798	Based on its phenotypic characteristics and on phylogenetic inference, strain AK73T represents a novel species of the genus Bacillus, for which the name Bacillus alkalilacus sp.
29616894	5	80	theme	high	845:848	arg1	abundance					850:858	a high abundance	843:858	a high abundance of iso-C15 	843:870	The fatty acids were dominated by branched iso-, anteiso-, saturated fatty acids with a high abundance of iso-C15 : 0, anteiso-C15 : 0, C16 : 0 and anteiso-C17 : 0; MK-7 was the major menaquinone.
29616894	6	81	contain	contained	982:990	arg1	peptidoglycan					968:980	The cell-wall peptidoglycan	954:980	The cell-wall peptidoglycan	954:980	The cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid.
29616894	6	81	contain	contained	982:990	arg2	acid					1043:1046	the diagnostic diamino acid	1020:1046	the diagnostic diamino acid	1020:1046	The cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid.
29616894	6	81	contain	contained	982:990	arg2	acid					1012:1015	meso-diaminopimelic acid	992:1015	meso-diaminopimelic acid	992:1015	The cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid.
29616894	2	82	theme	designated	188:197	arg1	bacterium					177:185	An aerobic, endospore-forming, haloalkali-tolerant, Gram-stain-positive, motile, rod-shaped bacterium	85:185	bacterium	177:185	An aerobic, endospore-forming, haloalkali-tolerant, Gram-stain-positive, motile, rod-shaped bacterium, designated strain AK73T, was isolated from a sediment sample collected from Sambhar lake, Jaipur, Rajasthan, India.
29616894	2	82	theme	designated	188:197	arg1	AK73T					206:210	designated strain AK73T	188:210	designated strain AK73T	188:210	An aerobic, endospore-forming, haloalkali-tolerant, Gram-stain-positive, motile, rod-shaped bacterium, designated strain AK73T, was isolated from a sediment sample collected from Sambhar lake, Jaipur, Rajasthan, India.
29616894	8	83	theme	DNA	1246:1248	arg1	G+C content					1250:1260	The DNA G+C content	1242:1260	The DNA G+C content of strain AK73T	1242:1276	The DNA G+C content of strain AK73T was 54 mol%.
29616894	8	83	theme	DNA	1246:1248	arg1	%					1288:1288	54 mol%	1282:1288	54 mol%	1282:1288	The DNA G+C content of strain AK73T was 54 mol%.
29616894	3	84	theme	marine	428:433	arg1	agar					435:438	marine agar	428:438	marine agar at pH 9	428:446	Colonies were circular, 1-2 mm in diameter, glossy, smooth, yellowish and convex with an entire margin after 48 h growth on marine agar at pH 9 and 37 °C. Growth occurred at 15-42 °C, 0-10 % (w/v) NaCl and at a pH range of 7-12.
29616894	7	85	theme	polar	1053:1057	arg1	lipids					1059:1064	The polar lipids	1049:1064	The polar lipids	1049:1064	The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, one unidentified aminophospholipid, four unidentified phospholipids and three unidentified lipids.
29616894	7	85	theme	polar	1053:1057	arg1	diphosphatidylglycerol					1071:1092	diphosphatidylglycerol	1071:1092	diphosphatidylglycerol	1071:1092	The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, one unidentified aminophospholipid, four unidentified phospholipids and three unidentified lipids.
29616894	10	86	theme	93.4 	1738:1742	arg1	%					1743:1743	93.6, 93.5 and 93.4 %	1723:1743	%	1743:1743	Phylogenetic analysis showed that strain AK73T formed a separate lineage but was loosely associated with a peripheral cluster of organisms that contained Bacillus gibsonii, Bacillus murimartini and Bacillus plakortidis with similarity values of 93.6, 93.5 and 93.4 %, respectively.
29616894	3	87	from	yellowish	364:372	arg1	circular					318:325	circular	318:325	circular	318:325	Colonies were circular, 1-2 mm in diameter, glossy, smooth, yellowish and convex with an entire margin after 48 h growth on marine agar at pH 9 and 37 °C. Growth occurred at 15-42 °C, 0-10 % (w/v) NaCl and at a pH range of 7-12.
29616894	5	88	theme	fatty	826:830	arg1	iso-					800:803	branched iso-	791:803	branched iso-	791:803	The fatty acids were dominated by branched iso-, anteiso-, saturated fatty acids with a high abundance of iso-C15 : 0, anteiso-C15 : 0, C16 : 0 and anteiso-C17 : 0; MK-7 was the major menaquinone.
29616894	5	88	theme	fatty	826:830	arg1	acids					832:836	saturated fatty acids	816:836	saturated fatty acids with a high abundance of iso-C15 	816:870	The fatty acids were dominated by branched iso-, anteiso-, saturated fatty acids with a high abundance of iso-C15 : 0, anteiso-C15 : 0, C16 : 0 and anteiso-C17 : 0; MK-7 was the major menaquinone.
29616894	5	89	with	acids	832:836	arg1	abundance					850:858	a high abundance	843:858	a high abundance of iso-C15 	843:870	The fatty acids were dominated by branched iso-, anteiso-, saturated fatty acids with a high abundance of iso-C15 : 0, anteiso-C15 : 0, C16 : 0 and anteiso-C17 : 0; MK-7 was the major menaquinone.
29616894	8	90	theme	AK73T	1272:1276	arg1	G+C content					1250:1260	The DNA G+C content	1242:1260	The DNA G+C content of strain AK73T	1242:1276	The DNA G+C content of strain AK73T was 54 mol%.
29616894	8	90	theme	AK73T	1272:1276	arg1	%					1288:1288	54 mol%	1282:1288	54 mol%	1282:1288	The DNA G+C content of strain AK73T was 54 mol%.
29616894	3	91	from	convex	378:383	arg1	circular					318:325	circular	318:325	circular	318:325	Colonies were circular, 1-2 mm in diameter, glossy, smooth, yellowish and convex with an entire margin after 48 h growth on marine agar at pH 9 and 37 °C. Growth occurred at 15-42 °C, 0-10 % (w/v) NaCl and at a pH range of 7-12.
31595527	2	0	theme	intestinal	467:476	arg1	microflora					478:487	intestinal microflora	467:487	intestinal microflora	467:487	The dextran sodium sulfate (DSS)-induced colitis mouse model was established to investigate the correlation between the protective effects of CEO and the regulation of intestinal microflora.
31595527	8	1	theme	flora	1628:1632	arg1	imbalance					1634:1642	the intestinal flora imbalance	1613:1642	the intestinal flora imbalance	1613:1642	These findings indicated from a new perspective that the inhibitory effect of CEO on IBD was closely related to improving the intestinal flora imbalance.
31595527	2	2	theme	-induced	331:338	arg1	model					354:358	The dextran sodium sulfate (DSS)-induced colitis mouse model	299:358	The dextran sodium sulfate (DSS)-induced colitis mouse model	299:358	The dextran sodium sulfate (DSS)-induced colitis mouse model was established to investigate the correlation between the protective effects of CEO and the regulation of intestinal microflora.
31595527	0	3	theme	mouse	58:62	arg1	model					64:68	the mouse model	54:68	the mouse model of dextran sodium	54:86	Effect of cinnamon essential oil on gut microbiota in the mouse model of dextran sodium sulfate-induced colitis.
31595527	6	4	theme	modified	1080:1087	arg1	composition					1099:1109	a modified community composition	1078:1109	a modified community composition	1078:1109	In contrast to the inability of antibiotics to regulate flora imbalance, the mice fed with CEO had an improved diversity and richness of intestinal microbiota, and a modified community composition with a decrease in Helicobacter and Bacteroides and an increase in Bacteroidales_S24-7 family and short-chain fatty acids (SCFA)-producing bacteria (Alloprevotella and Lachnospiraceae_NK4A136_group).
31595527	6	5	dep	antibiotics	946:956	arg1	regulate					961:968	regulate	961:968	to regulate flora imbalance	958:984	In contrast to the inability of antibiotics to regulate flora imbalance, the mice fed with CEO had an improved diversity and richness of intestinal microbiota, and a modified community composition with a decrease in Helicobacter and Bacteroides and an increase in Bacteroidales_S24-7 family and short-chain fatty acids (SCFA)-producing bacteria (Alloprevotella and Lachnospiraceae_NK4A136_group).
31595527	0	6	theme	dextran	73:79	arg1	sodium					81:86	dextran sodium	73:86	dextran sodium	73:86	Effect of cinnamon essential oil on gut microbiota in the mouse model of dextran sodium sulfate-induced colitis.
31595527	0	7	from	Effect	0:5	arg1	microbiota					40:49	gut microbiota	36:49	gut microbiota in the mouse model of dextran sodium	36:86	Effect of cinnamon essential oil on gut microbiota in the mouse model of dextran sodium sulfate-induced colitis.
31595527	5	8	theme	colitis	905:911	arg1	development					878:888	the development	874:888	the development of DSS-induced colitis	874:911	The results indicated that the oral administration of CEO enriched with cinnamaldehyde effectively alleviated the development of DSS-induced colitis.
31595527	0	9	from	microbiota	40:49	arg1	model					64:68	the mouse model	54:68	the mouse model of dextran sodium	54:86	Effect of cinnamon essential oil on gut microbiota in the mouse model of dextran sodium sulfate-induced colitis.
31595527	6	10	theme	Bacteroidales_S24-7	1178:1196	arg1	family					1198:1203	Bacteroidales_S24-7 family	1178:1203	Bacteroidales_S24-7 family	1178:1203	In contrast to the inability of antibiotics to regulate flora imbalance, the mice fed with CEO had an improved diversity and richness of intestinal microbiota, and a modified community composition with a decrease in Helicobacter and Bacteroides and an increase in Bacteroidales_S24-7 family and short-chain fatty acids (SCFA)-producing bacteria (Alloprevotella and Lachnospiraceae_NK4A136_group).
31595527	6	11	theme	fatty	1221:1225	arg1	SCFA					1234:1237	SCFA	1234:1237	SCFA	1234:1237	In contrast to the inability of antibiotics to regulate flora imbalance, the mice fed with CEO had an improved diversity and richness of intestinal microbiota, and a modified community composition with a decrease in Helicobacter and Bacteroides and an increase in Bacteroidales_S24-7 family and short-chain fatty acids (SCFA)-producing bacteria (Alloprevotella and Lachnospiraceae_NK4A136_group).
31595527	6	11	theme	fatty	1221:1225	arg1	acids					1227:1231	short-chain fatty acids	1209:1231	short-chain fatty acids (SCFA)	1209:1238	In contrast to the inability of antibiotics to regulate flora imbalance, the mice fed with CEO had an improved diversity and richness of intestinal microbiota, and a modified community composition with a decrease in Helicobacter and Bacteroides and an increase in Bacteroidales_S24-7 family and short-chain fatty acids (SCFA)-producing bacteria (Alloprevotella and Lachnospiraceae_NK4A136_group).
31595527	4	12	theme	gene	747:750	arg1	sequencing					752:761	rRNA gene sequencing	742:761	rRNA gene sequencing	742:761	The alteration of the fecal microbiome composition was analyzed by 16S rRNA gene sequencing.
31595527	1	13	theme	inflammatory	265:276	arg1	IBD					293:295	IBD	293:295	IBD	293:295	Increasing evidence has confirmed that the antimicrobial and anti-inflammatory effects of cinnamon essential oil (CEO) contribute to protection against inflammatory bowel disease (IBD).
31595527	1	13	theme	inflammatory	265:276	arg1	disease					284:290	inflammatory bowel disease	265:290	inflammatory bowel disease (IBD)	265:296	Increasing evidence has confirmed that the antimicrobial and anti-inflammatory effects of cinnamon essential oil (CEO) contribute to protection against inflammatory bowel disease (IBD).
31595527	8	14	theme	CEO	1569:1571	arg1	effect					1559:1564	the inhibitory effect	1544:1564	the inhibitory effect of CEO on IBD	1544:1578	These findings indicated from a new perspective that the inhibitory effect of CEO on IBD was closely related to improving the intestinal flora imbalance.
31595527	8	14	theme	CEO	1569:1571	arg1	related					1592:1598	related	1592:1598	related	1592:1598	These findings indicated from a new perspective that the inhibitory effect of CEO on IBD was closely related to improving the intestinal flora imbalance.
31595527	6	15	theme	community	1089:1097	arg1	composition					1099:1109	a modified community composition	1078:1109	a modified community composition	1078:1109	In contrast to the inability of antibiotics to regulate flora imbalance, the mice fed with CEO had an improved diversity and richness of intestinal microbiota, and a modified community composition with a decrease in Helicobacter and Bacteroides and an increase in Bacteroidales_S24-7 family and short-chain fatty acids (SCFA)-producing bacteria (Alloprevotella and Lachnospiraceae_NK4A136_group).
31595527	6	16	from	increase	1166:1173	arg1	SCFA					1234:1237	SCFA	1234:1237	SCFA	1234:1237	In contrast to the inability of antibiotics to regulate flora imbalance, the mice fed with CEO had an improved diversity and richness of intestinal microbiota, and a modified community composition with a decrease in Helicobacter and Bacteroides and an increase in Bacteroidales_S24-7 family and short-chain fatty acids (SCFA)-producing bacteria (Alloprevotella and Lachnospiraceae_NK4A136_group).
31595527	6	16	from	increase	1166:1173	arg1	acids					1227:1231	short-chain fatty acids	1209:1231	short-chain fatty acids (SCFA)	1209:1238	In contrast to the inability of antibiotics to regulate flora imbalance, the mice fed with CEO had an improved diversity and richness of intestinal microbiota, and a modified community composition with a decrease in Helicobacter and Bacteroides and an increase in Bacteroidales_S24-7 family and short-chain fatty acids (SCFA)-producing bacteria (Alloprevotella and Lachnospiraceae_NK4A136_group).
31595527	6	16	from	increase	1166:1173	arg1	family					1198:1203	Bacteroidales_S24-7 family	1178:1203	Bacteroidales_S24-7 family	1178:1203	In contrast to the inability of antibiotics to regulate flora imbalance, the mice fed with CEO had an improved diversity and richness of intestinal microbiota, and a modified community composition with a decrease in Helicobacter and Bacteroides and an increase in Bacteroidales_S24-7 family and short-chain fatty acids (SCFA)-producing bacteria (Alloprevotella and Lachnospiraceae_NK4A136_group).
31595527	6	16	from	increase	1166:1173	arg1	Bacteroides					1147:1157	Bacteroides	1147:1157	Bacteroides	1147:1157	In contrast to the inability of antibiotics to regulate flora imbalance, the mice fed with CEO had an improved diversity and richness of intestinal microbiota, and a modified community composition with a decrease in Helicobacter and Bacteroides and an increase in Bacteroidales_S24-7 family and short-chain fatty acids (SCFA)-producing bacteria (Alloprevotella and Lachnospiraceae_NK4A136_group).
31595527	6	16	from	increase	1166:1173	arg1	Helicobacter					1130:1141	Helicobacter	1130:1141	Helicobacter	1130:1141	In contrast to the inability of antibiotics to regulate flora imbalance, the mice fed with CEO had an improved diversity and richness of intestinal microbiota, and a modified community composition with a decrease in Helicobacter and Bacteroides and an increase in Bacteroidales_S24-7 family and short-chain fatty acids (SCFA)-producing bacteria (Alloprevotella and Lachnospiraceae_NK4A136_group).
31595527	1	17	theme	antimicrobial	156:168	arg1	effects					192:198	the antimicrobial and anti-inflammatory effects	152:198	the antimicrobial and anti-inflammatory effects of cinnamon essential oil (CEO)	152:230	Increasing evidence has confirmed that the antimicrobial and anti-inflammatory effects of cinnamon essential oil (CEO) contribute to protection against inflammatory bowel disease (IBD).
31595527	0	18	theme	sodium	81:86	arg1	model					64:68	the mouse model	54:68	the mouse model of dextran sodium	54:86	Effect of cinnamon essential oil on gut microbiota in the mouse model of dextran sodium sulfate-induced colitis.
31595527	7	19	theme	SCFA-producing	1466:1479	arg1	bacteria					1481:1488	SCFA-producing bacteria	1466:1488	SCFA-producing bacteria	1466:1488	Moreover, the correlation analysis showed that TLR4 and tumor necrosis factor-α was positively correlated with Helicobacter, but inversely correlated with SCFA-producing bacteria.
31595527	5	20	theme	oral	795:798	arg1	administration					800:813	the oral administration	791:813	the oral administration of CEO enriched with cinnamaldehyde	791:849	The results indicated that the oral administration of CEO enriched with cinnamaldehyde effectively alleviated the development of DSS-induced colitis.
31595527	1	21	theme	anti-inflammatory	174:190	arg1	effects					192:198	the antimicrobial and anti-inflammatory effects	152:198	the antimicrobial and anti-inflammatory effects of cinnamon essential oil (CEO)	152:230	Increasing evidence has confirmed that the antimicrobial and anti-inflammatory effects of cinnamon essential oil (CEO) contribute to protection against inflammatory bowel disease (IBD).
31595527	8	22	from	effect	1559:1564	arg1	IBD					1576:1578	IBD	1576:1578	IBD	1576:1578	These findings indicated from a new perspective that the inhibitory effect of CEO on IBD was closely related to improving the intestinal flora imbalance.
31595527	1	23	theme	bowel	278:282	arg1	IBD					293:295	IBD	293:295	IBD	293:295	Increasing evidence has confirmed that the antimicrobial and anti-inflammatory effects of cinnamon essential oil (CEO) contribute to protection against inflammatory bowel disease (IBD).
31595527	1	23	theme	bowel	278:282	arg1	disease					284:290	inflammatory bowel disease	265:290	inflammatory bowel disease (IBD)	265:296	Increasing evidence has confirmed that the antimicrobial and anti-inflammatory effects of cinnamon essential oil (CEO) contribute to protection against inflammatory bowel disease (IBD).
31595527	4	24	theme	composition	710:720	arg1	alteration					675:684	The alteration	671:684	The alteration of the fecal microbiome composition	671:720	The alteration of the fecal microbiome composition was analyzed by 16S rRNA gene sequencing.
31595527	8	25	theme	new	1523:1525	arg1	perspective					1527:1537	a new perspective	1521:1537	a new perspective	1521:1537	These findings indicated from a new perspective that the inhibitory effect of CEO on IBD was closely related to improving the intestinal flora imbalance.
31595527	6	26	theme	microbiota	1062:1071	arg1	composition					1099:1109	a modified community composition	1078:1109	a modified community composition	1078:1109	In contrast to the inability of antibiotics to regulate flora imbalance, the mice fed with CEO had an improved diversity and richness of intestinal microbiota, and a modified community composition with a decrease in Helicobacter and Bacteroides and an increase in Bacteroidales_S24-7 family and short-chain fatty acids (SCFA)-producing bacteria (Alloprevotella and Lachnospiraceae_NK4A136_group).
31595527	6	26	theme	microbiota	1062:1071	arg1	richness					1039:1046	richness	1039:1046	richness of intestinal microbiota	1039:1071	In contrast to the inability of antibiotics to regulate flora imbalance, the mice fed with CEO had an improved diversity and richness of intestinal microbiota, and a modified community composition with a decrease in Helicobacter and Bacteroides and an increase in Bacteroidales_S24-7 family and short-chain fatty acids (SCFA)-producing bacteria (Alloprevotella and Lachnospiraceae_NK4A136_group).
31595527	6	26	theme	microbiota	1062:1071	arg1	diversity					1025:1033	an improved diversity	1013:1033	an improved diversity	1013:1033	In contrast to the inability of antibiotics to regulate flora imbalance, the mice fed with CEO had an improved diversity and richness of intestinal microbiota, and a modified community composition with a decrease in Helicobacter and Bacteroides and an increase in Bacteroidales_S24-7 family and short-chain fatty acids (SCFA)-producing bacteria (Alloprevotella and Lachnospiraceae_NK4A136_group).
31595527	2	27	theme	protective	419:428	arg1	effects					430:436	the protective effects	415:436	the protective effects of CEO	415:443	The dextran sodium sulfate (DSS)-induced colitis mouse model was established to investigate the correlation between the protective effects of CEO and the regulation of intestinal microflora.
31595527	7	28	theme	tumor	1367:1371	arg1	factor-α					1382:1389	tumor necrosis factor-α	1367:1389	tumor necrosis factor-α	1367:1389	Moreover, the correlation analysis showed that TLR4 and tumor necrosis factor-α was positively correlated with Helicobacter, but inversely correlated with SCFA-producing bacteria.
31595527	4	29	theme	rRNA	742:745	arg1	sequencing					752:761	rRNA gene sequencing	742:761	rRNA gene sequencing	742:761	The alteration of the fecal microbiome composition was analyzed by 16S rRNA gene sequencing.
31595527	6	30	theme	intestinal	1051:1060	arg1	microbiota					1062:1071	intestinal microbiota	1051:1071	intestinal microbiota	1051:1071	In contrast to the inability of antibiotics to regulate flora imbalance, the mice fed with CEO had an improved diversity and richness of intestinal microbiota, and a modified community composition with a decrease in Helicobacter and Bacteroides and an increase in Bacteroidales_S24-7 family and short-chain fatty acids (SCFA)-producing bacteria (Alloprevotella and Lachnospiraceae_NK4A136_group).
31595527	0	31	theme	cinnamon	10:17	arg1	oil					29:31	cinnamon essential oil	10:31	cinnamon essential oil	10:31	Effect of cinnamon essential oil on gut microbiota in the mouse model of dextran sodium sulfate-induced colitis.
31595527	3	32	theme	receptor	643:650	arg1	expression					659:668	toll-like receptor (TLR4) expression	633:668	toll-like receptor (TLR4) expression	633:668	The symptoms of IBD were assessed by measuring the hemoglobin content, myeloperoxidase activity, histopathological observation, cytokines, and toll-like receptor (TLR4) expression.
31595527	3	33	theme	hemoglobin	541:550	arg1	content					552:558	the hemoglobin content	537:558	the hemoglobin content	537:558	The symptoms of IBD were assessed by measuring the hemoglobin content, myeloperoxidase activity, histopathological observation, cytokines, and toll-like receptor (TLR4) expression.
31595527	1	34	theme	cinnamon	203:210	arg1	CEO					227:229	CEO	227:229	CEO	227:229	Increasing evidence has confirmed that the antimicrobial and anti-inflammatory effects of cinnamon essential oil (CEO) contribute to protection against inflammatory bowel disease (IBD).
31595527	1	34	theme	cinnamon	203:210	arg1	oil					222:224	cinnamon essential oil	203:224	cinnamon essential oil (CEO)	203:230	Increasing evidence has confirmed that the antimicrobial and anti-inflammatory effects of cinnamon essential oil (CEO) contribute to protection against inflammatory bowel disease (IBD).
31595527	6	35	theme	flora	970:974	arg1	imbalance					976:984	flora imbalance	970:984	flora imbalance	970:984	In contrast to the inability of antibiotics to regulate flora imbalance, the mice fed with CEO had an improved diversity and richness of intestinal microbiota, and a modified community composition with a decrease in Helicobacter and Bacteroides and an increase in Bacteroidales_S24-7 family and short-chain fatty acids (SCFA)-producing bacteria (Alloprevotella and Lachnospiraceae_NK4A136_group).
31595527	6	36	dep	family	1198:1203	arg1	Alloprevotella					1260:1273	Alloprevotella	1260:1273	Alloprevotella	1260:1273	In contrast to the inability of antibiotics to regulate flora imbalance, the mice fed with CEO had an improved diversity and richness of intestinal microbiota, and a modified community composition with a decrease in Helicobacter and Bacteroides and an increase in Bacteroidales_S24-7 family and short-chain fatty acids (SCFA)-producing bacteria (Alloprevotella and Lachnospiraceae_NK4A136_group).
31595527	6	36	dep	family	1198:1203	arg1	bacteria					1250:1257	-producing bacteria	1239:1257	-producing bacteria (Alloprevotella and Lachnospiraceae_NK4A136_group)	1239:1308	In contrast to the inability of antibiotics to regulate flora imbalance, the mice fed with CEO had an improved diversity and richness of intestinal microbiota, and a modified community composition with a decrease in Helicobacter and Bacteroides and an increase in Bacteroidales_S24-7 family and short-chain fatty acids (SCFA)-producing bacteria (Alloprevotella and Lachnospiraceae_NK4A136_group).
31595527	6	36	dep	family	1198:1203	arg1	Lachnospiraceae_NK4A136_group					1279:1307	Lachnospiraceae_NK4A136_group	1279:1307	Lachnospiraceae_NK4A136_group	1279:1307	In contrast to the inability of antibiotics to regulate flora imbalance, the mice fed with CEO had an improved diversity and richness of intestinal microbiota, and a modified community composition with a decrease in Helicobacter and Bacteroides and an increase in Bacteroidales_S24-7 family and short-chain fatty acids (SCFA)-producing bacteria (Alloprevotella and Lachnospiraceae_NK4A136_group).
31595527	6	37	theme	-producing	1239:1248	arg1	Alloprevotella					1260:1273	Alloprevotella	1260:1273	Alloprevotella	1260:1273	In contrast to the inability of antibiotics to regulate flora imbalance, the mice fed with CEO had an improved diversity and richness of intestinal microbiota, and a modified community composition with a decrease in Helicobacter and Bacteroides and an increase in Bacteroidales_S24-7 family and short-chain fatty acids (SCFA)-producing bacteria (Alloprevotella and Lachnospiraceae_NK4A136_group).
31595527	6	37	theme	-producing	1239:1248	arg1	bacteria					1250:1257	-producing bacteria	1239:1257	-producing bacteria (Alloprevotella and Lachnospiraceae_NK4A136_group)	1239:1308	In contrast to the inability of antibiotics to regulate flora imbalance, the mice fed with CEO had an improved diversity and richness of intestinal microbiota, and a modified community composition with a decrease in Helicobacter and Bacteroides and an increase in Bacteroidales_S24-7 family and short-chain fatty acids (SCFA)-producing bacteria (Alloprevotella and Lachnospiraceae_NK4A136_group).
31595527	6	37	theme	-producing	1239:1248	arg1	Lachnospiraceae_NK4A136_group					1279:1307	Lachnospiraceae_NK4A136_group	1279:1307	Lachnospiraceae_NK4A136_group	1279:1307	In contrast to the inability of antibiotics to regulate flora imbalance, the mice fed with CEO had an improved diversity and richness of intestinal microbiota, and a modified community composition with a decrease in Helicobacter and Bacteroides and an increase in Bacteroidales_S24-7 family and short-chain fatty acids (SCFA)-producing bacteria (Alloprevotella and Lachnospiraceae_NK4A136_group).
31595527	1	38	theme	essential	212:220	arg1	CEO					227:229	CEO	227:229	CEO	227:229	Increasing evidence has confirmed that the antimicrobial and anti-inflammatory effects of cinnamon essential oil (CEO) contribute to protection against inflammatory bowel disease (IBD).
31595527	1	38	theme	essential	212:220	arg1	oil					222:224	cinnamon essential oil	203:224	cinnamon essential oil (CEO)	203:230	Increasing evidence has confirmed that the antimicrobial and anti-inflammatory effects of cinnamon essential oil (CEO) contribute to protection against inflammatory bowel disease (IBD).
31595527	0	39	theme	oil	29:31	arg1	Effect					0:5	Effect	0:5	Effect of cinnamon essential oil on gut microbiota in the mouse model of dextran sodium	0:86	Effect of cinnamon essential oil on gut microbiota in the mouse model of dextran sodium sulfate-induced colitis.
31595527	8	40	theme	intestinal	1617:1626	arg1	imbalance					1634:1642	the intestinal flora imbalance	1613:1642	the intestinal flora imbalance	1613:1642	These findings indicated from a new perspective that the inhibitory effect of CEO on IBD was closely related to improving the intestinal flora imbalance.
31595527	0	41	theme	essential	19:27	arg1	oil					29:31	cinnamon essential oil	10:31	cinnamon essential oil	10:31	Effect of cinnamon essential oil on gut microbiota in the mouse model of dextran sodium sulfate-induced colitis.
31595527	6	42	from	decrease	1118:1125	arg1	SCFA					1234:1237	SCFA	1234:1237	SCFA	1234:1237	In contrast to the inability of antibiotics to regulate flora imbalance, the mice fed with CEO had an improved diversity and richness of intestinal microbiota, and a modified community composition with a decrease in Helicobacter and Bacteroides and an increase in Bacteroidales_S24-7 family and short-chain fatty acids (SCFA)-producing bacteria (Alloprevotella and Lachnospiraceae_NK4A136_group).
31595527	6	42	from	decrease	1118:1125	arg1	acids					1227:1231	short-chain fatty acids	1209:1231	short-chain fatty acids (SCFA)	1209:1238	In contrast to the inability of antibiotics to regulate flora imbalance, the mice fed with CEO had an improved diversity and richness of intestinal microbiota, and a modified community composition with a decrease in Helicobacter and Bacteroides and an increase in Bacteroidales_S24-7 family and short-chain fatty acids (SCFA)-producing bacteria (Alloprevotella and Lachnospiraceae_NK4A136_group).
31595527	6	42	from	decrease	1118:1125	arg1	family					1198:1203	Bacteroidales_S24-7 family	1178:1203	Bacteroidales_S24-7 family	1178:1203	In contrast to the inability of antibiotics to regulate flora imbalance, the mice fed with CEO had an improved diversity and richness of intestinal microbiota, and a modified community composition with a decrease in Helicobacter and Bacteroides and an increase in Bacteroidales_S24-7 family and short-chain fatty acids (SCFA)-producing bacteria (Alloprevotella and Lachnospiraceae_NK4A136_group).
31595527	6	42	from	decrease	1118:1125	arg1	Bacteroides					1147:1157	Bacteroides	1147:1157	Bacteroides	1147:1157	In contrast to the inability of antibiotics to regulate flora imbalance, the mice fed with CEO had an improved diversity and richness of intestinal microbiota, and a modified community composition with a decrease in Helicobacter and Bacteroides and an increase in Bacteroidales_S24-7 family and short-chain fatty acids (SCFA)-producing bacteria (Alloprevotella and Lachnospiraceae_NK4A136_group).
31595527	6	42	from	decrease	1118:1125	arg1	Helicobacter					1130:1141	Helicobacter	1130:1141	Helicobacter	1130:1141	In contrast to the inability of antibiotics to regulate flora imbalance, the mice fed with CEO had an improved diversity and richness of intestinal microbiota, and a modified community composition with a decrease in Helicobacter and Bacteroides and an increase in Bacteroidales_S24-7 family and short-chain fatty acids (SCFA)-producing bacteria (Alloprevotella and Lachnospiraceae_NK4A136_group).
31595527	1	43	theme	oil	222:224	arg1	effects					192:198	the antimicrobial and anti-inflammatory effects	152:198	the antimicrobial and anti-inflammatory effects of cinnamon essential oil (CEO)	152:230	Increasing evidence has confirmed that the antimicrobial and anti-inflammatory effects of cinnamon essential oil (CEO) contribute to protection against inflammatory bowel disease (IBD).
31595527	5	44	theme	DSS-induced	893:903	arg1	colitis					905:911	DSS-induced colitis	893:911	DSS-induced colitis	893:911	The results indicated that the oral administration of CEO enriched with cinnamaldehyde effectively alleviated the development of DSS-induced colitis.
31595527	3	45	theme	toll-like	633:641	arg1	receptor					643:650	toll-like receptor	633:650	toll-like receptor (TLR4) expression	633:668	The symptoms of IBD were assessed by measuring the hemoglobin content, myeloperoxidase activity, histopathological observation, cytokines, and toll-like receptor (TLR4) expression.
31595527	3	45	theme	toll-like	633:641	arg1	TLR4					653:656	TLR4	653:656	TLR4	653:656	The symptoms of IBD were assessed by measuring the hemoglobin content, myeloperoxidase activity, histopathological observation, cytokines, and toll-like receptor (TLR4) expression.
31595527	3	46	theme	myeloperoxidase	561:575	arg1	activity					577:584	myeloperoxidase activity	561:584	myeloperoxidase activity	561:584	The symptoms of IBD were assessed by measuring the hemoglobin content, myeloperoxidase activity, histopathological observation, cytokines, and toll-like receptor (TLR4) expression.
31595527	4	47	theme	microbiome	699:708	arg1	composition					710:720	the fecal microbiome composition	689:720	the fecal microbiome composition	689:720	The alteration of the fecal microbiome composition was analyzed by 16S rRNA gene sequencing.
31595527	0	48	theme	gut	36:38	arg1	microbiota					40:49	gut microbiota	36:49	gut microbiota in the mouse model of dextran sodium	36:86	Effect of cinnamon essential oil on gut microbiota in the mouse model of dextran sodium sulfate-induced colitis.
31595527	6	49	theme	antibiotics	946:956	arg1	inability					933:941	the inability	929:941	the inability of antibiotics to regulate flora imbalance	929:984	In contrast to the inability of antibiotics to regulate flora imbalance, the mice fed with CEO had an improved diversity and richness of intestinal microbiota, and a modified community composition with a decrease in Helicobacter and Bacteroides and an increase in Bacteroidales_S24-7 family and short-chain fatty acids (SCFA)-producing bacteria (Alloprevotella and Lachnospiraceae_NK4A136_group).
31595527	2	50	theme	CEO	441:443	arg1	effects					430:436	the protective effects	415:436	the protective effects of CEO	415:443	The dextran sodium sulfate (DSS)-induced colitis mouse model was established to investigate the correlation between the protective effects of CEO and the regulation of intestinal microflora.
31595527	2	50	theme	CEO	441:443	arg1	regulation					453:462	the regulation	449:462	the regulation of intestinal microflora	449:487	The dextran sodium sulfate (DSS)-induced colitis mouse model was established to investigate the correlation between the protective effects of CEO and the regulation of intestinal microflora.
31595527	4	51	theme	fecal	693:697	arg1	composition					710:720	the fecal microbiome composition	689:720	the fecal microbiome composition	689:720	The alteration of the fecal microbiome composition was analyzed by 16S rRNA gene sequencing.
31595527	7	52	theme	necrosis	1373:1380	arg1	factor-α					1382:1389	tumor necrosis factor-α	1367:1389	tumor necrosis factor-α	1367:1389	Moreover, the correlation analysis showed that TLR4 and tumor necrosis factor-α was positively correlated with Helicobacter, but inversely correlated with SCFA-producing bacteria.
31595527	3	53	theme	histopathological	587:603	arg1	observation					605:615	histopathological observation	587:615	histopathological observation	587:615	The symptoms of IBD were assessed by measuring the hemoglobin content, myeloperoxidase activity, histopathological observation, cytokines, and toll-like receptor (TLR4) expression.
31595527	1	54	theme	Increasing	113:122	arg1	evidence					124:131	Increasing evidence	113:131	Increasing evidence	113:131	Increasing evidence has confirmed that the antimicrobial and anti-inflammatory effects of cinnamon essential oil (CEO) contribute to protection against inflammatory bowel disease (IBD).
31595527	6	55	dep	bacteria	1250:1257	arg1	Alloprevotella					1260:1273	Alloprevotella	1260:1273	Alloprevotella	1260:1273	In contrast to the inability of antibiotics to regulate flora imbalance, the mice fed with CEO had an improved diversity and richness of intestinal microbiota, and a modified community composition with a decrease in Helicobacter and Bacteroides and an increase in Bacteroidales_S24-7 family and short-chain fatty acids (SCFA)-producing bacteria (Alloprevotella and Lachnospiraceae_NK4A136_group).
31595527	6	55	dep	bacteria	1250:1257	arg1	bacteria					1250:1257	-producing bacteria	1239:1257	-producing bacteria (Alloprevotella and Lachnospiraceae_NK4A136_group)	1239:1308	In contrast to the inability of antibiotics to regulate flora imbalance, the mice fed with CEO had an improved diversity and richness of intestinal microbiota, and a modified community composition with a decrease in Helicobacter and Bacteroides and an increase in Bacteroidales_S24-7 family and short-chain fatty acids (SCFA)-producing bacteria (Alloprevotella and Lachnospiraceae_NK4A136_group).
31595527	6	55	dep	bacteria	1250:1257	arg1	Lachnospiraceae_NK4A136_group					1279:1307	Lachnospiraceae_NK4A136_group	1279:1307	Lachnospiraceae_NK4A136_group	1279:1307	In contrast to the inability of antibiotics to regulate flora imbalance, the mice fed with CEO had an improved diversity and richness of intestinal microbiota, and a modified community composition with a decrease in Helicobacter and Bacteroides and an increase in Bacteroidales_S24-7 family and short-chain fatty acids (SCFA)-producing bacteria (Alloprevotella and Lachnospiraceae_NK4A136_group).
31595527	6	56	contain	had	1009:1011	arg2	diversity					1025:1033	an improved diversity	1013:1033	an improved diversity	1013:1033	In contrast to the inability of antibiotics to regulate flora imbalance, the mice fed with CEO had an improved diversity and richness of intestinal microbiota, and a modified community composition with a decrease in Helicobacter and Bacteroides and an increase in Bacteroidales_S24-7 family and short-chain fatty acids (SCFA)-producing bacteria (Alloprevotella and Lachnospiraceae_NK4A136_group).
31595527	6	56	contain	had	1009:1011	arg1	mice					991:994	the mice	987:994	the mice fed with CEO	987:1007	In contrast to the inability of antibiotics to regulate flora imbalance, the mice fed with CEO had an improved diversity and richness of intestinal microbiota, and a modified community composition with a decrease in Helicobacter and Bacteroides and an increase in Bacteroidales_S24-7 family and short-chain fatty acids (SCFA)-producing bacteria (Alloprevotella and Lachnospiraceae_NK4A136_group).
31595527	6	56	contain	had	1009:1011	arg2	richness					1039:1046	richness	1039:1046	richness of intestinal microbiota	1039:1071	In contrast to the inability of antibiotics to regulate flora imbalance, the mice fed with CEO had an improved diversity and richness of intestinal microbiota, and a modified community composition with a decrease in Helicobacter and Bacteroides and an increase in Bacteroidales_S24-7 family and short-chain fatty acids (SCFA)-producing bacteria (Alloprevotella and Lachnospiraceae_NK4A136_group).
31595527	6	56	contain	had	1009:1011	arg2	composition					1099:1109	a modified community composition	1078:1109	a modified community composition	1078:1109	In contrast to the inability of antibiotics to regulate flora imbalance, the mice fed with CEO had an improved diversity and richness of intestinal microbiota, and a modified community composition with a decrease in Helicobacter and Bacteroides and an increase in Bacteroidales_S24-7 family and short-chain fatty acids (SCFA)-producing bacteria (Alloprevotella and Lachnospiraceae_NK4A136_group).
31595527	3	57	theme	IBD	506:508	arg1	symptoms					494:501	The symptoms	490:501	The symptoms of IBD	490:508	The symptoms of IBD were assessed by measuring the hemoglobin content, myeloperoxidase activity, histopathological observation, cytokines, and toll-like receptor (TLR4) expression.
31595527	6	58	theme	improved	1016:1023	arg1	diversity					1025:1033	an improved diversity	1013:1033	an improved diversity	1013:1033	In contrast to the inability of antibiotics to regulate flora imbalance, the mice fed with CEO had an improved diversity and richness of intestinal microbiota, and a modified community composition with a decrease in Helicobacter and Bacteroides and an increase in Bacteroidales_S24-7 family and short-chain fatty acids (SCFA)-producing bacteria (Alloprevotella and Lachnospiraceae_NK4A136_group).
31595527	6	59	theme	short-chain	1209:1219	arg1	SCFA					1234:1237	SCFA	1234:1237	SCFA	1234:1237	In contrast to the inability of antibiotics to regulate flora imbalance, the mice fed with CEO had an improved diversity and richness of intestinal microbiota, and a modified community composition with a decrease in Helicobacter and Bacteroides and an increase in Bacteroidales_S24-7 family and short-chain fatty acids (SCFA)-producing bacteria (Alloprevotella and Lachnospiraceae_NK4A136_group).
31595527	6	59	theme	short-chain	1209:1219	arg1	acids					1227:1231	short-chain fatty acids	1209:1231	short-chain fatty acids (SCFA)	1209:1238	In contrast to the inability of antibiotics to regulate flora imbalance, the mice fed with CEO had an improved diversity and richness of intestinal microbiota, and a modified community composition with a decrease in Helicobacter and Bacteroides and an increase in Bacteroidales_S24-7 family and short-chain fatty acids (SCFA)-producing bacteria (Alloprevotella and Lachnospiraceae_NK4A136_group).
31595527	7	60	theme	correlation	1325:1335	arg1	analysis					1337:1344	the correlation analysis	1321:1344	the correlation analysis	1321:1344	Moreover, the correlation analysis showed that TLR4 and tumor necrosis factor-α was positively correlated with Helicobacter, but inversely correlated with SCFA-producing bacteria.
31595527	2	61	theme	mouse	348:352	arg1	model					354:358	The dextran sodium sulfate (DSS)-induced colitis mouse model	299:358	The dextran sodium sulfate (DSS)-induced colitis mouse model	299:358	The dextran sodium sulfate (DSS)-induced colitis mouse model was established to investigate the correlation between the protective effects of CEO and the regulation of intestinal microflora.
31595527	2	62	theme	microflora	478:487	arg1	effects					430:436	the protective effects	415:436	the protective effects of CEO	415:443	The dextran sodium sulfate (DSS)-induced colitis mouse model was established to investigate the correlation between the protective effects of CEO and the regulation of intestinal microflora.
31595527	2	62	theme	microflora	478:487	arg1	regulation					453:462	the regulation	449:462	the regulation of intestinal microflora	449:487	The dextran sodium sulfate (DSS)-induced colitis mouse model was established to investigate the correlation between the protective effects of CEO and the regulation of intestinal microflora.
31595527	8	63	theme	inhibitory	1548:1557	arg1	effect					1559:1564	the inhibitory effect	1544:1564	the inhibitory effect of CEO on IBD	1544:1578	These findings indicated from a new perspective that the inhibitory effect of CEO on IBD was closely related to improving the intestinal flora imbalance.
31595527	8	63	theme	inhibitory	1548:1557	arg1	related					1592:1598	related	1592:1598	related	1592:1598	These findings indicated from a new perspective that the inhibitory effect of CEO on IBD was closely related to improving the intestinal flora imbalance.
31595527	5	64	theme	CEO	818:820	arg1	administration					800:813	the oral administration	791:813	the oral administration of CEO enriched with cinnamaldehyde	791:849	The results indicated that the oral administration of CEO enriched with cinnamaldehyde effectively alleviated the development of DSS-induced colitis.
31595527	2	65	theme	colitis	340:346	arg1	model					354:358	The dextran sodium sulfate (DSS)-induced colitis mouse model	299:358	The dextran sodium sulfate (DSS)-induced colitis mouse model	299:358	The dextran sodium sulfate (DSS)-induced colitis mouse model was established to investigate the correlation between the protective effects of CEO and the regulation of intestinal microflora.
30252445	8	0	theme	spectra	1174:1180	arg1	interpretation					1124:1137	interpretation	1124:1137	interpretation	1124:1137	glyXtoolMS is built around the pipeline engine of OpenMS (TOPPAS) and provides a glycopeptide analysis toolbox for the analysis, interpretation, and visualization of glycopeptide spectra.
30252445	8	0	theme	spectra	1174:1180	arg1	visualization					1144:1156	visualization	1144:1156	visualization	1144:1156	glyXtoolMS is built around the pipeline engine of OpenMS (TOPPAS) and provides a glycopeptide analysis toolbox for the analysis, interpretation, and visualization of glycopeptide spectra.
30252445	8	0	theme	spectra	1174:1180	arg1	analysis					1114:1121	analysis	1114:1121	analysis	1114:1121	glyXtoolMS is built around the pipeline engine of OpenMS (TOPPAS) and provides a glycopeptide analysis toolbox for the analysis, interpretation, and visualization of glycopeptide spectra.
30252445	11	1	from	digests	1792:1798	arg1	data					1724:1727	higher-energy collisional dissociation data	1685:1727	higher-energy collisional dissociation data from human immunoglobulin γ (IgG) and human fibrinogen tryptic digests	1685:1798	Using higher-energy collisional dissociation data from human immunoglobulin γ (IgG) and human fibrinogen tryptic digests, we show that glyXtoolMS enables a fast, flexible, and transparent analysis of N- and O-glycopeptide samples, providing the user a versatile tool even for explorative data analysis.
30252445	11	2	theme	data	1967:1970	arg1	analysis					1972:1979	explorative data analysis	1955:1979	explorative data analysis	1955:1979	Using higher-energy collisional dissociation data from human immunoglobulin γ (IgG) and human fibrinogen tryptic digests, we show that glyXtoolMS enables a fast, flexible, and transparent analysis of N- and O-glycopeptide samples, providing the user a versatile tool even for explorative data analysis.
30252445	7	3	theme	Python	875:880	arg1	suite					891:895	the open-source Python software suite	859:895	the open-source Python software suite glyXtoolMS	859:906	Here, we present the open-source Python software suite glyXtoolMS, developed for the semiautomated analysis of N- and O-glycopeptide fragmentation data.
30252445	9	4	theme	peptide	1419:1425	arg1	sequences					1427:1435	peptide sequences	1419:1435	peptide sequences	1419:1435	The toolbox encompasses (a) filtering of fragment spectra using a scoring scheme for oxonium ions, (b) in silico digest of protein sequences to collect glycopeptide candidates, (c) precursor matching to possible glycan compositions and peptide sequences, and finally, (d) an annotation tool for glycopeptide fragment ions.
30252445	7	5	dep	suite	891:895	arg1	glyXtoolMS					897:906	glyXtoolMS	897:906	the open-source Python software suite glyXtoolMS	859:906	Here, we present the open-source Python software suite glyXtoolMS, developed for the semiautomated analysis of N- and O-glycopeptide fragmentation data.
30252445	2	6	theme	cumbersome	276:285	arg1	spectra					311:317	the time-consuming, cumbersome, and error-prone manual spectra	256:317	the time-consuming, cumbersome, and error-prone manual spectra analysis and glycopeptide identification	256:358	These tools support or replace the time-consuming, cumbersome, and error-prone manual spectra analysis and glycopeptide identification.
30252445	2	6	theme	cumbersome	276:285	arg1	analysis					319:326	analysis	319:326	analysis	319:326	These tools support or replace the time-consuming, cumbersome, and error-prone manual spectra analysis and glycopeptide identification.
30252445	2	6	theme	cumbersome	276:285	arg1	identification					345:358	glycopeptide identification	332:358	glycopeptide identification	332:358	These tools support or replace the time-consuming, cumbersome, and error-prone manual spectra analysis and glycopeptide identification.
30252445	9	7	from	ions	1276:1279	arg1	digest					1296:1301	silico digest	1289:1301	silico digest of protein sequences to collect glycopeptide candidates, (c) precursor matching to possible glycan compositions and peptide sequences, and finally, (d) an annotation tool for glycopeptide fragment ions	1289:1503	The toolbox encompasses (a) filtering of fragment spectra using a scoring scheme for oxonium ions, (b) in silico digest of protein sequences to collect glycopeptide candidates, (c) precursor matching to possible glycan compositions and peptide sequences, and finally, (d) an annotation tool for glycopeptide fragment ions.
30252445	8	8	theme	pipeline	1026:1033	arg1	engine					1035:1040	the pipeline engine	1022:1040	the pipeline engine of OpenMS (TOPPAS)	1022:1059	glyXtoolMS is built around the pipeline engine of OpenMS (TOPPAS) and provides a glycopeptide analysis toolbox for the analysis, interpretation, and visualization of glycopeptide spectra.
30252445	10	9	theme	MS	1568:1569	arg1	Evaluator					1571:1579	the glyXtool MS Evaluator	1555:1579	the glyXtool MS Evaluator	1555:1579	The resulting analysis file can be visualized by the glyXtool MS Evaluator, enabling further manual analysis, including inspection, verification, and various other options.
30252445	11	10	theme	fibrinogen	1773:1782	arg1	digests					1792:1798	human immunoglobulin γ (IgG) and human fibrinogen tryptic digests	1734:1798	human immunoglobulin γ (IgG) and human fibrinogen tryptic digests	1734:1798	Using higher-energy collisional dissociation data from human immunoglobulin γ (IgG) and human fibrinogen tryptic digests, we show that glyXtoolMS enables a fast, flexible, and transparent analysis of N- and O-glycopeptide samples, providing the user a versatile tool even for explorative data analysis.
30252445	6	11	theme	control	803:809	arg1	maximum					776:782	a maximum	774:782	a maximum of flexibility and control	774:809	This gives users a maximum of flexibility and control over the progress of analyses.
30252445	2	12	theme	manual	304:309	arg1	spectra					311:317	the time-consuming, cumbersome, and error-prone manual spectra	256:317	the time-consuming, cumbersome, and error-prone manual spectra analysis and glycopeptide identification	256:358	These tools support or replace the time-consuming, cumbersome, and error-prone manual spectra analysis and glycopeptide identification.
30252445	2	12	theme	manual	304:309	arg1	analysis					319:326	analysis	319:326	analysis	319:326	These tools support or replace the time-consuming, cumbersome, and error-prone manual spectra analysis and glycopeptide identification.
30252445	2	12	theme	manual	304:309	arg1	identification					345:358	glycopeptide identification	332:358	glycopeptide identification	332:358	These tools support or replace the time-consuming, cumbersome, and error-prone manual spectra analysis and glycopeptide identification.
30252445	1	13	theme	recent	211:216	arg1	years					218:222	the recent years	207:222	the recent years	207:222	For glycoproteomic analyses several web tools and standalone software packages have been developed over the recent years.
30252445	1	14	theme	several	131:137	arg1	tools					143:147	several web tools	131:147	several web tools	131:147	For glycoproteomic analyses several web tools and standalone software packages have been developed over the recent years.
30252445	10	15	theme	further	1591:1597	arg1	analysis					1606:1613	further manual analysis	1591:1613	further manual analysis	1591:1613	The resulting analysis file can be visualized by the glyXtool MS Evaluator, enabling further manual analysis, including inspection, verification, and various other options.
30252445	13	16	theme	test	2113:2116	arg1	available					2127:2135	available	2127:2135	available	2127:2135	The test data are available via ProteomeXchange with identifier PXD009716.
30252445	13	16	theme	test	2113:2116	arg1	data					2118:2121	The test data	2109:2121	The test data	2109:2121	The test data are available via ProteomeXchange with identifier PXD009716.
30252445	11	17	theme	user	1924:1927	arg1	tool					1941:1944	the user a versatile tool	1920:1944	the user a versatile tool	1920:1944	Using higher-energy collisional dissociation data from human immunoglobulin γ (IgG) and human fibrinogen tryptic digests, we show that glyXtoolMS enables a fast, flexible, and transparent analysis of N- and O-glycopeptide samples, providing the user a versatile tool even for explorative data analysis.
30252445	2	18	dep	spectra	311:317	arg1	spectra					311:317	the time-consuming, cumbersome, and error-prone manual spectra	256:317	the time-consuming, cumbersome, and error-prone manual spectra analysis and glycopeptide identification	256:358	These tools support or replace the time-consuming, cumbersome, and error-prone manual spectra analysis and glycopeptide identification.
30252445	2	18	dep	spectra	311:317	arg1	analysis					319:326	analysis	319:326	analysis	319:326	These tools support or replace the time-consuming, cumbersome, and error-prone manual spectra analysis and glycopeptide identification.
30252445	2	18	dep	spectra	311:317	arg1	identification					345:358	glycopeptide identification	332:358	glycopeptide identification	332:358	These tools support or replace the time-consuming, cumbersome, and error-prone manual spectra analysis and glycopeptide identification.
30252445	9	19	theme	oxonium	1268:1274	arg1	ions					1276:1279	oxonium ions	1268:1279	oxonium ions, (b) in silico digest of protein sequences to collect glycopeptide candidates, (c) precursor matching to possible glycan compositions and peptide sequences, and finally, (d) an annotation tool for glycopeptide fragment ions	1268:1503	The toolbox encompasses (a) filtering of fragment spectra using a scoring scheme for oxonium ions, (b) in silico digest of protein sequences to collect glycopeptide candidates, (c) precursor matching to possible glycan compositions and peptide sequences, and finally, (d) an annotation tool for glycopeptide fragment ions.
30252445	9	19	theme	oxonium	1268:1274	arg1	b					1283:1283	b	1283:1283	b	1283:1283	The toolbox encompasses (a) filtering of fragment spectra using a scoring scheme for oxonium ions, (b) in silico digest of protein sequences to collect glycopeptide candidates, (c) precursor matching to possible glycan compositions and peptide sequences, and finally, (d) an annotation tool for glycopeptide fragment ions.
30252445	9	20	theme	annotation	1458:1467	arg1	tool					1469:1472	an annotation tool	1455:1472	(d) an annotation tool for glycopeptide fragment ions	1451:1503	The toolbox encompasses (a) filtering of fragment spectra using a scoring scheme for oxonium ions, (b) in silico digest of protein sequences to collect glycopeptide candidates, (c) precursor matching to possible glycan compositions and peptide sequences, and finally, (d) an annotation tool for glycopeptide fragment ions.
30252445	11	21	theme	human	1734:1738	arg1	immunoglobulin					1740:1753	human immunoglobulin γ	1734:1755	human immunoglobulin γ (IgG)	1734:1761	Using higher-energy collisional dissociation data from human immunoglobulin γ (IgG) and human fibrinogen tryptic digests, we show that glyXtoolMS enables a fast, flexible, and transparent analysis of N- and O-glycopeptide samples, providing the user a versatile tool even for explorative data analysis.
30252445	11	21	theme	human	1734:1738	arg1	IgG					1758:1760	IgG	1758:1760	IgG	1758:1760	Using higher-energy collisional dissociation data from human immunoglobulin γ (IgG) and human fibrinogen tryptic digests, we show that glyXtoolMS enables a fast, flexible, and transparent analysis of N- and O-glycopeptide samples, providing the user a versatile tool even for explorative data analysis.
30252445	2	22	theme	time-consuming	260:273	arg1	spectra					311:317	the time-consuming, cumbersome, and error-prone manual spectra	256:317	the time-consuming, cumbersome, and error-prone manual spectra analysis and glycopeptide identification	256:358	These tools support or replace the time-consuming, cumbersome, and error-prone manual spectra analysis and glycopeptide identification.
30252445	2	22	theme	time-consuming	260:273	arg1	analysis					319:326	analysis	319:326	analysis	319:326	These tools support or replace the time-consuming, cumbersome, and error-prone manual spectra analysis and glycopeptide identification.
30252445	2	22	theme	time-consuming	260:273	arg1	identification					345:358	glycopeptide identification	332:358	glycopeptide identification	332:358	These tools support or replace the time-consuming, cumbersome, and error-prone manual spectra analysis and glycopeptide identification.
30252445	11	23	theme	versatile	1931:1939	arg1	tool					1941:1944	the user a versatile tool	1920:1944	the user a versatile tool	1920:1944	Using higher-energy collisional dissociation data from human immunoglobulin γ (IgG) and human fibrinogen tryptic digests, we show that glyXtoolMS enables a fast, flexible, and transparent analysis of N- and O-glycopeptide samples, providing the user a versatile tool even for explorative data analysis.
30252445	12	24	from	online	2013:2018	arg1	https					2023:2027	https	2023:2027	https	2023:2027	glyXtoolMS is freely available online on https://github.com/glyXera/glyXtoolMS licensed under the GPL-3.0 open-source license.
30252445	9	25	theme	fragment	1491:1498	arg1	ions					1500:1503	glycopeptide fragment ions	1478:1503	glycopeptide fragment ions	1478:1503	The toolbox encompasses (a) filtering of fragment spectra using a scoring scheme for oxonium ions, (b) in silico digest of protein sequences to collect glycopeptide candidates, (c) precursor matching to possible glycan compositions and peptide sequences, and finally, (d) an annotation tool for glycopeptide fragment ions.
30252445	11	26	gly	O-glycopeptide	1886:1899	arg2	O-glycopeptide					1886:1899	O-glycopeptide samples	1886:1907	O-glycopeptide samples	1886:1907	Using higher-energy collisional dissociation data from human immunoglobulin γ (IgG) and human fibrinogen tryptic digests, we show that glyXtoolMS enables a fast, flexible, and transparent analysis of N- and O-glycopeptide samples, providing the user a versatile tool even for explorative data analysis.
30252445	0	27	theme	Mass	79:82	arg1	Data					97:100	Glycopeptide Mass Spectrometry Data	66:100	Glycopeptide Mass Spectrometry Data	66:100	glyXtoolMS: An Open-Source Pipeline for Semiautomated Analysis of Glycopeptide Mass Spectrometry Data.
30252445	5	28	theme	analysis	628:635	arg1	tasks					637:641	the analysis tasks	624:641	the analysis tasks	624:641	We solved this problem by dividing the analysis tasks into modular tools with defined functions, which are executed within a software pipeline with a graphical editor.
30252445	3	29	theme	software	379:386	arg1	tools					388:392	existing software tools	370:392	existing software tools	370:392	However, existing software tools are usually tailored to one fragmentation technique and only present the final analysis results.
30252445	4	30	theme	intermediate	538:549	arg1	results					551:557	intermediate results	538:557	intermediate results	538:557	This makes manual inspection and correction of intermediate results difficult or even impossible.
30252445	9	31	dep	tool	1469:1472	arg1	d					1452:1452	d	1452:1452	d	1452:1452	The toolbox encompasses (a) filtering of fragment spectra using a scoring scheme for oxonium ions, (b) in silico digest of protein sequences to collect glycopeptide candidates, (c) precursor matching to possible glycan compositions and peptide sequences, and finally, (d) an annotation tool for glycopeptide fragment ions.
30252445	0	32	theme	Data	97:100	arg1	Analysis					54:61	Semiautomated Analysis	40:61	Semiautomated Analysis of Glycopeptide Mass Spectrometry Data	40:100	glyXtoolMS: An Open-Source Pipeline for Semiautomated Analysis of Glycopeptide Mass Spectrometry Data.
30252445	7	33	theme	fragmentation	975:987	arg1	data					989:992	O-glycopeptide fragmentation data	960:992	O-glycopeptide fragmentation data	960:992	Here, we present the open-source Python software suite glyXtoolMS, developed for the semiautomated analysis of N- and O-glycopeptide fragmentation data.
30252445	9	34	gly	glycopeptide	1478:1489	arg2	glycopeptide					1478:1489	glycopeptide	1478:1489	glycopeptide	1478:1489	The toolbox encompasses (a) filtering of fragment spectra using a scoring scheme for oxonium ions, (b) in silico digest of protein sequences to collect glycopeptide candidates, (c) precursor matching to possible glycan compositions and peptide sequences, and finally, (d) an annotation tool for glycopeptide fragment ions.
30252445	9	35	theme	protein	1306:1312	arg1	sequences					1314:1322	protein sequences	1306:1322	protein sequences	1306:1322	The toolbox encompasses (a) filtering of fragment spectra using a scoring scheme for oxonium ions, (b) in silico digest of protein sequences to collect glycopeptide candidates, (c) precursor matching to possible glycan compositions and peptide sequences, and finally, (d) an annotation tool for glycopeptide fragment ions.
30252445	5	36	with	pipeline	723:730	arg1	editor					749:754	a graphical editor	737:754	a graphical editor	737:754	We solved this problem by dividing the analysis tasks into modular tools with defined functions, which are executed within a software pipeline with a graphical editor.
30252445	8	37	theme	glycopeptide	1076:1087	arg1	toolbox					1098:1104	a glycopeptide analysis toolbox	1074:1104	a glycopeptide analysis toolbox	1074:1104	glyXtoolMS is built around the pipeline engine of OpenMS (TOPPAS) and provides a glycopeptide analysis toolbox for the analysis, interpretation, and visualization of glycopeptide spectra.
30252445	11	38	theme	O-glycopeptide	1886:1899	arg1	samples					1901:1907	O-glycopeptide samples	1886:1907	O-glycopeptide samples	1886:1907	Using higher-energy collisional dissociation data from human immunoglobulin γ (IgG) and human fibrinogen tryptic digests, we show that glyXtoolMS enables a fast, flexible, and transparent analysis of N- and O-glycopeptide samples, providing the user a versatile tool even for explorative data analysis.
30252445	9	39	theme	glycopeptide	1335:1346	arg1	candidates					1348:1357	glycopeptide candidates	1335:1357	glycopeptide candidates	1335:1357	The toolbox encompasses (a) filtering of fragment spectra using a scoring scheme for oxonium ions, (b) in silico digest of protein sequences to collect glycopeptide candidates, (c) precursor matching to possible glycan compositions and peptide sequences, and finally, (d) an annotation tool for glycopeptide fragment ions.
30252445	1	40	theme	glycoproteomic	107:120	arg1	analyses					122:129	glycoproteomic analyses	107:129	glycoproteomic analyses	107:129	For glycoproteomic analyses several web tools and standalone software packages have been developed over the recent years.
30252445	11	41	theme	N-	1879:1880	arg1	analysis					1867:1874	a fast, flexible, and transparent analysis	1833:1874	a fast, flexible, and transparent analysis of N- and O-glycopeptide samples	1833:1907	Using higher-energy collisional dissociation data from human immunoglobulin γ (IgG) and human fibrinogen tryptic digests, we show that glyXtoolMS enables a fast, flexible, and transparent analysis of N- and O-glycopeptide samples, providing the user a versatile tool even for explorative data analysis.
30252445	3	42	theme	analysis	473:480	arg1	results					482:488	the final analysis results	463:488	the final analysis results	463:488	However, existing software tools are usually tailored to one fragmentation technique and only present the final analysis results.
30252445	0	43	theme	Semiautomated	40:52	arg1	Analysis					54:61	Semiautomated Analysis	40:61	Semiautomated Analysis of Glycopeptide Mass Spectrometry Data	40:100	glyXtoolMS: An Open-Source Pipeline for Semiautomated Analysis of Glycopeptide Mass Spectrometry Data.
30252445	2	44	gly	glycopeptide	332:343	arg2	glycopeptide					332:343	glycopeptide identification	332:358	glycopeptide identification	332:358	These tools support or replace the time-consuming, cumbersome, and error-prone manual spectra analysis and glycopeptide identification.
30252445	11	45	theme	dissociation	1711:1722	arg1	data					1724:1727	higher-energy collisional dissociation data	1685:1727	higher-energy collisional dissociation data from human immunoglobulin γ (IgG) and human fibrinogen tryptic digests	1685:1798	Using higher-energy collisional dissociation data from human immunoglobulin γ (IgG) and human fibrinogen tryptic digests, we show that glyXtoolMS enables a fast, flexible, and transparent analysis of N- and O-glycopeptide samples, providing the user a versatile tool even for explorative data analysis.
30252445	10	46	theme	other	1664:1668	arg1	options					1670:1676	various other options	1656:1676	various other options	1656:1676	The resulting analysis file can be visualized by the glyXtool MS Evaluator, enabling further manual analysis, including inspection, verification, and various other options.
30252445	11	47	theme	explorative	1955:1965	arg1	analysis					1972:1979	explorative data analysis	1955:1979	explorative data analysis	1955:1979	Using higher-energy collisional dissociation data from human immunoglobulin γ (IgG) and human fibrinogen tryptic digests, we show that glyXtoolMS enables a fast, flexible, and transparent analysis of N- and O-glycopeptide samples, providing the user a versatile tool even for explorative data analysis.
30252445	1	48	theme	standalone	153:162	arg1	packages					173:180	standalone software packages	153:180	standalone software packages	153:180	For glycoproteomic analyses several web tools and standalone software packages have been developed over the recent years.
30252445	11	49	theme	higher-energy	1685:1697	arg1	data					1724:1727	higher-energy collisional dissociation data	1685:1727	higher-energy collisional dissociation data from human immunoglobulin γ (IgG) and human fibrinogen tryptic digests	1685:1798	Using higher-energy collisional dissociation data from human immunoglobulin γ (IgG) and human fibrinogen tryptic digests, we show that glyXtoolMS enables a fast, flexible, and transparent analysis of N- and O-glycopeptide samples, providing the user a versatile tool even for explorative data analysis.
30252445	11	50	theme	flexible	1841:1848	arg1	analysis					1867:1874	a fast, flexible, and transparent analysis	1833:1874	a fast, flexible, and transparent analysis of N- and O-glycopeptide samples	1833:1907	Using higher-energy collisional dissociation data from human immunoglobulin γ (IgG) and human fibrinogen tryptic digests, we show that glyXtoolMS enables a fast, flexible, and transparent analysis of N- and O-glycopeptide samples, providing the user a versatile tool even for explorative data analysis.
30252445	0	51	dep	glyXtoolMS	0:9	arg1	Pipeline					27:34	An Open-Source Pipeline	12:34	glyXtoolMS: An Open-Source Pipeline for Semiautomated Analysis of Glycopeptide Mass Spectrometry Data.	0:101	glyXtoolMS: An Open-Source Pipeline for Semiautomated Analysis of Glycopeptide Mass Spectrometry Data.
30252445	10	52	theme	resulting	1510:1518	arg1	file					1529:1532	The resulting analysis file	1506:1532	The resulting analysis file	1506:1532	The resulting analysis file can be visualized by the glyXtool MS Evaluator, enabling further manual analysis, including inspection, verification, and various other options.
30252445	11	53	theme	fast	1835:1838	arg1	analysis					1867:1874	a fast, flexible, and transparent analysis	1833:1874	a fast, flexible, and transparent analysis of N- and O-glycopeptide samples	1833:1907	Using higher-energy collisional dissociation data from human immunoglobulin γ (IgG) and human fibrinogen tryptic digests, we show that glyXtoolMS enables a fast, flexible, and transparent analysis of N- and O-glycopeptide samples, providing the user a versatile tool even for explorative data analysis.
30252445	9	54	theme	glycan	1395:1400	arg1	compositions					1402:1413	possible glycan compositions	1386:1413	possible glycan compositions	1386:1413	The toolbox encompasses (a) filtering of fragment spectra using a scoring scheme for oxonium ions, (b) in silico digest of protein sequences to collect glycopeptide candidates, (c) precursor matching to possible glycan compositions and peptide sequences, and finally, (d) an annotation tool for glycopeptide fragment ions.
30252445	8	55	theme	glycopeptide	1161:1172	arg1	spectra					1174:1180	glycopeptide spectra	1161:1180	glycopeptide spectra	1161:1180	glyXtoolMS is built around the pipeline engine of OpenMS (TOPPAS) and provides a glycopeptide analysis toolbox for the analysis, interpretation, and visualization of glycopeptide spectra.
30252445	6	56	theme	analyses	832:839	arg1	progress					820:827	the progress	816:827	the progress of analyses	816:839	This gives users a maximum of flexibility and control over the progress of analyses.
30252445	2	57	theme	error-prone	292:302	arg1	spectra					311:317	the time-consuming, cumbersome, and error-prone manual spectra	256:317	the time-consuming, cumbersome, and error-prone manual spectra analysis and glycopeptide identification	256:358	These tools support or replace the time-consuming, cumbersome, and error-prone manual spectra analysis and glycopeptide identification.
30252445	2	57	theme	error-prone	292:302	arg1	analysis					319:326	analysis	319:326	analysis	319:326	These tools support or replace the time-consuming, cumbersome, and error-prone manual spectra analysis and glycopeptide identification.
30252445	2	57	theme	error-prone	292:302	arg1	identification					345:358	glycopeptide identification	332:358	glycopeptide identification	332:358	These tools support or replace the time-consuming, cumbersome, and error-prone manual spectra analysis and glycopeptide identification.
30252445	7	58	theme	software	882:889	arg1	suite					891:895	the open-source Python software suite	859:895	the open-source Python software suite glyXtoolMS	859:906	Here, we present the open-source Python software suite glyXtoolMS, developed for the semiautomated analysis of N- and O-glycopeptide fragmentation data.
30252445	5	59	theme	defined	667:673	arg1	functions					675:683	defined functions	667:683	defined functions	667:683	We solved this problem by dividing the analysis tasks into modular tools with defined functions, which are executed within a software pipeline with a graphical editor.
30252445	9	60	theme	fragment	1224:1231	arg1	spectra					1233:1239	fragment spectra	1224:1239	fragment spectra using a scoring scheme for oxonium ions, (b) in silico digest of protein sequences to collect glycopeptide candidates, (c) precursor matching to possible glycan compositions and peptide sequences, and finally, (d) an annotation tool for glycopeptide fragment ions	1224:1503	The toolbox encompasses (a) filtering of fragment spectra using a scoring scheme for oxonium ions, (b) in silico digest of protein sequences to collect glycopeptide candidates, (c) precursor matching to possible glycan compositions and peptide sequences, and finally, (d) an annotation tool for glycopeptide fragment ions.
30252445	7	61	theme	open-source	863:873	arg1	suite					891:895	the open-source Python software suite	859:895	the open-source Python software suite glyXtoolMS	859:906	Here, we present the open-source Python software suite glyXtoolMS, developed for the semiautomated analysis of N- and O-glycopeptide fragmentation data.
30252445	8	62	theme	OpenMS	1045:1050	arg1	engine					1035:1040	the pipeline engine	1022:1040	the pipeline engine of OpenMS (TOPPAS)	1022:1059	glyXtoolMS is built around the pipeline engine of OpenMS (TOPPAS) and provides a glycopeptide analysis toolbox for the analysis, interpretation, and visualization of glycopeptide spectra.
30252445	10	63	theme	glyXtool	1559:1566	arg1	Evaluator					1571:1579	the glyXtool MS Evaluator	1555:1579	the glyXtool MS Evaluator	1555:1579	The resulting analysis file can be visualized by the glyXtool MS Evaluator, enabling further manual analysis, including inspection, verification, and various other options.
30252445	9	64	theme	spectra	1233:1239	arg1	filtering					1211:1219	(a) filtering	1207:1219	(a) filtering of fragment spectra using a scoring scheme for oxonium ions, (b) in silico digest of protein sequences to collect glycopeptide candidates, (c) precursor matching to possible glycan compositions and peptide sequences, and finally, (d) an annotation tool for glycopeptide fragment ions	1207:1503	The toolbox encompasses (a) filtering of fragment spectra using a scoring scheme for oxonium ions, (b) in silico digest of protein sequences to collect glycopeptide candidates, (c) precursor matching to possible glycan compositions and peptide sequences, and finally, (d) an annotation tool for glycopeptide fragment ions.
30252445	7	65	theme	data	989:992	arg1	analysis					941:948	the semiautomated analysis	923:948	the semiautomated analysis of N- and O-glycopeptide fragmentation data	923:992	Here, we present the open-source Python software suite glyXtoolMS, developed for the semiautomated analysis of N- and O-glycopeptide fragmentation data.
30252445	10	66	theme	manual	1599:1604	arg1	analysis					1606:1613	further manual analysis	1591:1613	further manual analysis	1591:1613	The resulting analysis file can be visualized by the glyXtool MS Evaluator, enabling further manual analysis, including inspection, verification, and various other options.
30252445	11	67	theme	human	1767:1771	arg1	fibrinogen					1773:1782	human fibrinogen	1767:1782	human fibrinogen	1767:1782	Using higher-energy collisional dissociation data from human immunoglobulin γ (IgG) and human fibrinogen tryptic digests, we show that glyXtoolMS enables a fast, flexible, and transparent analysis of N- and O-glycopeptide samples, providing the user a versatile tool even for explorative data analysis.
30252445	5	68	theme	graphical	739:747	arg1	editor					749:754	a graphical editor	737:754	a graphical editor	737:754	We solved this problem by dividing the analysis tasks into modular tools with defined functions, which are executed within a software pipeline with a graphical editor.
30252445	9	69	theme	scoring	1249:1255	arg1	scheme					1257:1262	a scoring scheme	1247:1262	a scoring scheme for oxonium ions, (b) in silico digest of protein sequences to collect glycopeptide candidates, (c) precursor matching to possible glycan compositions and peptide sequences, and finally, (d) an annotation tool for glycopeptide fragment ions	1247:1503	The toolbox encompasses (a) filtering of fragment spectra using a scoring scheme for oxonium ions, (b) in silico digest of protein sequences to collect glycopeptide candidates, (c) precursor matching to possible glycan compositions and peptide sequences, and finally, (d) an annotation tool for glycopeptide fragment ions.
30252445	9	70	dep	precursor	1364:1372	arg1	c					1361:1361	c	1361:1361	c	1361:1361	The toolbox encompasses (a) filtering of fragment spectra using a scoring scheme for oxonium ions, (b) in silico digest of protein sequences to collect glycopeptide candidates, (c) precursor matching to possible glycan compositions and peptide sequences, and finally, (d) an annotation tool for glycopeptide fragment ions.
30252445	11	71	theme	tryptic	1784:1790	arg1	digests					1792:1798	human immunoglobulin γ (IgG) and human fibrinogen tryptic digests	1734:1798	human immunoglobulin γ (IgG) and human fibrinogen tryptic digests	1734:1798	Using higher-energy collisional dissociation data from human immunoglobulin γ (IgG) and human fibrinogen tryptic digests, we show that glyXtoolMS enables a fast, flexible, and transparent analysis of N- and O-glycopeptide samples, providing the user a versatile tool even for explorative data analysis.
30252445	5	72	theme	software	714:721	arg1	pipeline					723:730	a software pipeline	712:730	a software pipeline with a graphical editor	712:754	We solved this problem by dividing the analysis tasks into modular tools with defined functions, which are executed within a software pipeline with a graphical editor.
30252445	1	73	theme	web	139:141	arg1	tools					143:147	several web tools	131:147	several web tools	131:147	For glycoproteomic analyses several web tools and standalone software packages have been developed over the recent years.
30252445	9	74	dep	filtering	1211:1219	arg1	a					1208:1208	a	1208:1208	a	1208:1208	The toolbox encompasses (a) filtering of fragment spectra using a scoring scheme for oxonium ions, (b) in silico digest of protein sequences to collect glycopeptide candidates, (c) precursor matching to possible glycan compositions and peptide sequences, and finally, (d) an annotation tool for glycopeptide fragment ions.
30252445	11	75	theme	immunoglobulin	1740:1753	arg1	digests					1792:1798	human immunoglobulin γ (IgG) and human fibrinogen tryptic digests	1734:1798	human immunoglobulin γ (IgG) and human fibrinogen tryptic digests	1734:1798	Using higher-energy collisional dissociation data from human immunoglobulin γ (IgG) and human fibrinogen tryptic digests, we show that glyXtoolMS enables a fast, flexible, and transparent analysis of N- and O-glycopeptide samples, providing the user a versatile tool even for explorative data analysis.
30252445	0	76	theme	Glycopeptide	66:77	arg1	Data					97:100	Glycopeptide Mass Spectrometry Data	66:100	Glycopeptide Mass Spectrometry Data	66:100	glyXtoolMS: An Open-Source Pipeline for Semiautomated Analysis of Glycopeptide Mass Spectrometry Data.
30252445	11	77	theme	a	1929:1929	arg1	tool					1941:1944	the user a versatile tool	1920:1944	the user a versatile tool	1920:1944	Using higher-energy collisional dissociation data from human immunoglobulin γ (IgG) and human fibrinogen tryptic digests, we show that glyXtoolMS enables a fast, flexible, and transparent analysis of N- and O-glycopeptide samples, providing the user a versatile tool even for explorative data analysis.
30252445	9	78	theme	glycopeptide	1478:1489	arg1	ions					1500:1503	glycopeptide fragment ions	1478:1503	glycopeptide fragment ions	1478:1503	The toolbox encompasses (a) filtering of fragment spectra using a scoring scheme for oxonium ions, (b) in silico digest of protein sequences to collect glycopeptide candidates, (c) precursor matching to possible glycan compositions and peptide sequences, and finally, (d) an annotation tool for glycopeptide fragment ions.
30252445	12	79	theme	available	2003:2011	arg1	online					2013:2018	freely available online	1996:2018	freely available online on https	1996:2027	glyXtoolMS is freely available online on https://github.com/glyXera/glyXtoolMS licensed under the GPL-3.0 open-source license.
30252445	0	80	theme	Spectrometry	84:95	arg1	Data					97:100	Glycopeptide Mass Spectrometry Data	66:100	Glycopeptide Mass Spectrometry Data	66:100	glyXtoolMS: An Open-Source Pipeline for Semiautomated Analysis of Glycopeptide Mass Spectrometry Data.
30252445	1	81	theme	software	164:171	arg1	packages					173:180	standalone software packages	153:180	standalone software packages	153:180	For glycoproteomic analyses several web tools and standalone software packages have been developed over the recent years.
30252445	4	82	theme	results	551:557	arg1	inspection					509:518	manual inspection	502:518	manual inspection	502:518	This makes manual inspection and correction of intermediate results difficult or even impossible.
30252445	4	82	theme	results	551:557	arg1	correction					524:533	correction	524:533	correction	524:533	This makes manual inspection and correction of intermediate results difficult or even impossible.
30252445	8	83	gly	glycopeptide	1076:1087	arg2	glycopeptide					1076:1087	a glycopeptide analysis toolbox	1074:1104	a glycopeptide analysis toolbox	1074:1104	glyXtoolMS is built around the pipeline engine of OpenMS (TOPPAS) and provides a glycopeptide analysis toolbox for the analysis, interpretation, and visualization of glycopeptide spectra.
30252445	9	84	theme	silico	1289:1294	arg1	digest					1296:1301	silico digest	1289:1301	silico digest of protein sequences to collect glycopeptide candidates, (c) precursor matching to possible glycan compositions and peptide sequences, and finally, (d) an annotation tool for glycopeptide fragment ions	1289:1503	The toolbox encompasses (a) filtering of fragment spectra using a scoring scheme for oxonium ions, (b) in silico digest of protein sequences to collect glycopeptide candidates, (c) precursor matching to possible glycan compositions and peptide sequences, and finally, (d) an annotation tool for glycopeptide fragment ions.
30252445	4	85	theme	manual	502:507	arg1	inspection					509:518	manual inspection	502:518	manual inspection	502:518	This makes manual inspection and correction of intermediate results difficult or even impossible.
30252445	2	86	theme	glycopeptide	332:343	arg1	spectra					311:317	the time-consuming, cumbersome, and error-prone manual spectra	256:317	the time-consuming, cumbersome, and error-prone manual spectra analysis and glycopeptide identification	256:358	These tools support or replace the time-consuming, cumbersome, and error-prone manual spectra analysis and glycopeptide identification.
30252445	2	86	theme	glycopeptide	332:343	arg1	identification					345:358	glycopeptide identification	332:358	glycopeptide identification	332:358	These tools support or replace the time-consuming, cumbersome, and error-prone manual spectra analysis and glycopeptide identification.
30252445	5	87	theme	modular	648:654	arg1	tools					656:660	modular tools	648:660	modular tools with defined functions, which are executed within a software pipeline with a graphical editor	648:754	We solved this problem by dividing the analysis tasks into modular tools with defined functions, which are executed within a software pipeline with a graphical editor.
30252445	3	88	theme	existing	370:377	arg1	tools					388:392	existing software tools	370:392	existing software tools	370:392	However, existing software tools are usually tailored to one fragmentation technique and only present the final analysis results.
30252445	8	89	dep	analysis	1114:1121	arg1	the					1110:1112	the	1110:1112	the	1110:1112	glyXtoolMS is built around the pipeline engine of OpenMS (TOPPAS) and provides a glycopeptide analysis toolbox for the analysis, interpretation, and visualization of glycopeptide spectra.
30252445	9	90	gly	glycopeptide	1335:1346	arg2	glycopeptide					1335:1346	glycopeptide candidates	1335:1357	glycopeptide candidates	1335:1357	The toolbox encompasses (a) filtering of fragment spectra using a scoring scheme for oxonium ions, (b) in silico digest of protein sequences to collect glycopeptide candidates, (c) precursor matching to possible glycan compositions and peptide sequences, and finally, (d) an annotation tool for glycopeptide fragment ions.
30252445	3	91	theme	final	467:471	arg1	results					482:488	the final analysis results	463:488	the final analysis results	463:488	However, existing software tools are usually tailored to one fragmentation technique and only present the final analysis results.
30252445	9	92	theme	sequences	1314:1322	arg1	digest					1296:1301	silico digest	1289:1301	silico digest of protein sequences to collect glycopeptide candidates, (c) precursor matching to possible glycan compositions and peptide sequences, and finally, (d) an annotation tool for glycopeptide fragment ions	1289:1503	The toolbox encompasses (a) filtering of fragment spectra using a scoring scheme for oxonium ions, (b) in silico digest of protein sequences to collect glycopeptide candidates, (c) precursor matching to possible glycan compositions and peptide sequences, and finally, (d) an annotation tool for glycopeptide fragment ions.
30252445	8	93	theme	analysis	1089:1096	arg1	toolbox					1098:1104	a glycopeptide analysis toolbox	1074:1104	a glycopeptide analysis toolbox	1074:1104	glyXtoolMS is built around the pipeline engine of OpenMS (TOPPAS) and provides a glycopeptide analysis toolbox for the analysis, interpretation, and visualization of glycopeptide spectra.
30252445	7	94	theme	O-glycopeptide	960:973	arg1	data					989:992	O-glycopeptide fragmentation data	960:992	O-glycopeptide fragmentation data	960:992	Here, we present the open-source Python software suite glyXtoolMS, developed for the semiautomated analysis of N- and O-glycopeptide fragmentation data.
30252445	11	95	theme	transparent	1855:1865	arg1	analysis					1867:1874	a fast, flexible, and transparent analysis	1833:1874	a fast, flexible, and transparent analysis of N- and O-glycopeptide samples	1833:1907	Using higher-energy collisional dissociation data from human immunoglobulin γ (IgG) and human fibrinogen tryptic digests, we show that glyXtoolMS enables a fast, flexible, and transparent analysis of N- and O-glycopeptide samples, providing the user a versatile tool even for explorative data analysis.
30252445	0	96	theme	Open-Source	15:25	arg1	Pipeline					27:34	An Open-Source Pipeline	12:34	glyXtoolMS: An Open-Source Pipeline for Semiautomated Analysis of Glycopeptide Mass Spectrometry Data.	0:101	glyXtoolMS: An Open-Source Pipeline for Semiautomated Analysis of Glycopeptide Mass Spectrometry Data.
30252445	7	97	theme	N-	953:954	arg1	analysis					941:948	the semiautomated analysis	923:948	the semiautomated analysis of N- and O-glycopeptide fragmentation data	923:992	Here, we present the open-source Python software suite glyXtoolMS, developed for the semiautomated analysis of N- and O-glycopeptide fragmentation data.
30252445	11	98	theme	samples	1901:1907	arg1	analysis					1867:1874	a fast, flexible, and transparent analysis	1833:1874	a fast, flexible, and transparent analysis of N- and O-glycopeptide samples	1833:1907	Using higher-energy collisional dissociation data from human immunoglobulin γ (IgG) and human fibrinogen tryptic digests, we show that glyXtoolMS enables a fast, flexible, and transparent analysis of N- and O-glycopeptide samples, providing the user a versatile tool even for explorative data analysis.
30252445	6	99	theme	flexibility	787:797	arg1	maximum					776:782	a maximum	774:782	a maximum of flexibility and control	774:809	This gives users a maximum of flexibility and control over the progress of analyses.
30252445	13	100	with	ProteomeXchange	2141:2155	arg1	identifier					2162:2171	identifier PXD009716	2162:2181	identifier PXD009716	2162:2181	The test data are available via ProteomeXchange with identifier PXD009716.
30252445	3	101	theme	fragmentation	422:434	arg1	technique					436:444	one fragmentation technique	418:444	one fragmentation technique	418:444	However, existing software tools are usually tailored to one fragmentation technique and only present the final analysis results.
30252445	11	102	theme	collisional	1699:1709	arg1	data					1724:1727	higher-energy collisional dissociation data	1685:1727	higher-energy collisional dissociation data from human immunoglobulin γ (IgG) and human fibrinogen tryptic digests	1685:1798	Using higher-energy collisional dissociation data from human immunoglobulin γ (IgG) and human fibrinogen tryptic digests, we show that glyXtoolMS enables a fast, flexible, and transparent analysis of N- and O-glycopeptide samples, providing the user a versatile tool even for explorative data analysis.
30252445	8	103	gly	glycopeptide	1161:1172	arg2	glycopeptide					1161:1172	glycopeptide spectra	1161:1180	glycopeptide spectra	1161:1180	glyXtoolMS is built around the pipeline engine of OpenMS (TOPPAS) and provides a glycopeptide analysis toolbox for the analysis, interpretation, and visualization of glycopeptide spectra.
30252445	7	104	theme	semiautomated	927:939	arg1	analysis					941:948	the semiautomated analysis	923:948	the semiautomated analysis of N- and O-glycopeptide fragmentation data	923:992	Here, we present the open-source Python software suite glyXtoolMS, developed for the semiautomated analysis of N- and O-glycopeptide fragmentation data.
30252445	5	105	with	tools	656:660	arg1	functions					675:683	defined functions	667:683	defined functions	667:683	We solved this problem by dividing the analysis tasks into modular tools with defined functions, which are executed within a software pipeline with a graphical editor.
30252445	7	106	gly	O-glycopeptide	960:973	arg2	O-glycopeptide					960:973	O-glycopeptide fragmentation data	960:992	O-glycopeptide fragmentation data	960:992	Here, we present the open-source Python software suite glyXtoolMS, developed for the semiautomated analysis of N- and O-glycopeptide fragmentation data.
30252445	10	107	theme	various	1656:1662	arg1	options					1670:1676	various other options	1656:1676	various other options	1656:1676	The resulting analysis file can be visualized by the glyXtool MS Evaluator, enabling further manual analysis, including inspection, verification, and various other options.
30252445	10	108	theme	analysis	1520:1527	arg1	file					1529:1532	The resulting analysis file	1506:1532	The resulting analysis file	1506:1532	The resulting analysis file can be visualized by the glyXtool MS Evaluator, enabling further manual analysis, including inspection, verification, and various other options.
30252445	9	109	theme	possible	1386:1393	arg1	compositions					1402:1413	possible glycan compositions	1386:1413	possible glycan compositions	1386:1413	The toolbox encompasses (a) filtering of fragment spectra using a scoring scheme for oxonium ions, (b) in silico digest of protein sequences to collect glycopeptide candidates, (c) precursor matching to possible glycan compositions and peptide sequences, and finally, (d) an annotation tool for glycopeptide fragment ions.
30198753	2	0	theme	metabolic	334:342	arg1	profile					344:350	serum metabolic profile	328:350	serum metabolic profile	328:350	Two separate experiments were conducted to evaluate the efficacy of either prebiotic or synbiotic on growth performance, carcass yield, organ weights, and serum metabolic profile of broilers under hot climatic conditions.
30198753	10	1	from	gain	1415:1418	arg1	experiment					1515:1524	experiment 2	1515:1526	experiment 2	1515:1526	Similarly, supplementation of the prebiotic at 0.5 or 1 g kg-1 in broiler diets during 1-21 d and 0.25 or 0.50 g kg-1 feed, respectively, during 22-42 d improved (P < 0.01) body weight gain, FCR, and decreased (P < 0.01) abdominal fat, serum cholesterol, and LDL-cholesterol levels in experiment 2.
30198753	4	2	theme	diets	534:538	arg1	kg-1					561:564	0, 0.5 or 0.75 g kg-1	544:564	0, 0.5 or 0.75 g kg-1 of the grower diets	544:584	In experiment 1, 150 1-d-old Ross 308 male broiler chickens were fed dietary synbiotic (Biomin® IMBO, 0, 1 or 1.5 g kg-1 of the starter diets and 0, 0.5 or 0.75 g kg-1 of the grower diets) for 6 weeks.
30198753	4	2	theme	diets	534:538	arg1	kg-1					514:517	0, 1 or 1.5 g kg-1	500:517	0, 1 or 1.5 g kg-1 of the starter diets	500:538	In experiment 1, 150 1-d-old Ross 308 male broiler chickens were fed dietary synbiotic (Biomin® IMBO, 0, 1 or 1.5 g kg-1 of the starter diets and 0, 0.5 or 0.75 g kg-1 of the grower diets) for 6 weeks.
30198753	10	3	from	FCR	1421:1423	arg1	experiment					1515:1524	experiment 2	1515:1526	experiment 2	1515:1526	Similarly, supplementation of the prebiotic at 0.5 or 1 g kg-1 in broiler diets during 1-21 d and 0.25 or 0.50 g kg-1 feed, respectively, during 22-42 d improved (P < 0.01) body weight gain, FCR, and decreased (P < 0.01) abdominal fat, serum cholesterol, and LDL-cholesterol levels in experiment 2.
30198753	10	4	theme	22-42 d	1375:1381	arg1	gain					1415:1418	22-42 d improved (P < 0.01) body weight gain	1375:1418	22-42 d improved (P < 0.01) body weight gain	1375:1418	Similarly, supplementation of the prebiotic at 0.5 or 1 g kg-1 in broiler diets during 1-21 d and 0.25 or 0.50 g kg-1 feed, respectively, during 22-42 d improved (P < 0.01) body weight gain, FCR, and decreased (P < 0.01) abdominal fat, serum cholesterol, and LDL-cholesterol levels in experiment 2.
30198753	4	5	theme	grower	573:578	arg1	diets					580:584	the grower diets	569:584	the grower diets	569:584	In experiment 1, 150 1-d-old Ross 308 male broiler chickens were fed dietary synbiotic (Biomin® IMBO, 0, 1 or 1.5 g kg-1 of the starter diets and 0, 0.5 or 0.75 g kg-1 of the grower diets) for 6 weeks.
30198753	0	6	theme	Growth	0:5	arg1	performance					7:17	Growth performance	0:17	Growth performance	0:17	Growth performance, carcass criteria, and serum biochemical parameters of broiler chickens supplemented with either synbiotic or prebiotic under hot climatic conditions.
30198753	8	7	theme	weight	1050:1055	arg1	ratio					1079:1083	feed conversion ratio	1063:1083	feed conversion ratio (FCR)	1063:1089	Supplementation of synbiotic at 1 or 1.5 g kg-1 feed to broiler diets during 1-21 d and 0.5 or 0.75 g kg-1 feed, respectively, during 22-42 d improved (P < 0.01) body weight gain, feed conversion ratio (FCR), and decreased (P < 0.01) abdominal fat, serum total cholesterol, and low-density lipoprotein (LDL) cholesterol levels in experiment 1.
30198753	8	7	theme	weight	1050:1055	arg1	gain					1057:1060	22-42 d improved (P < 0.01) body weight gain	1017:1060	22-42 d improved (P < 0.01) body weight gain	1017:1060	Supplementation of synbiotic at 1 or 1.5 g kg-1 feed to broiler diets during 1-21 d and 0.5 or 0.75 g kg-1 feed, respectively, during 22-42 d improved (P < 0.01) body weight gain, feed conversion ratio (FCR), and decreased (P < 0.01) abdominal fat, serum total cholesterol, and low-density lipoprotein (LDL) cholesterol levels in experiment 1.
30198753	5	8	theme	diets	745:749	arg1	kg-1					725:728	0, 0.5 or 1 g kg-1	711:728	0, 0.5 or 1 g kg-1 of the starter diets	711:749	In experiment 2, 150 1-d-old Ross 308 male broiler chickens were fed dietary prebiotic (Mannan oligosaccharide 0, 0.5 or 1 g kg-1 of the starter diets and 0, 0.25 or 0.5 g kg-1 of the grower diets) for 6 weeks.
30198753	5	8	theme	diets	745:749	arg1	kg-1					772:775	0, 0.25 or 0.5 g kg-1	755:775	0, 0.25 or 0.5 g kg-1 of the grower	755:789	In experiment 2, 150 1-d-old Ross 308 male broiler chickens were fed dietary prebiotic (Mannan oligosaccharide 0, 0.5 or 1 g kg-1 of the starter diets and 0, 0.25 or 0.5 g kg-1 of the grower diets) for 6 weeks.
30198753	2	9	theme	carcass	294:300	arg1	yield					302:306	carcass yield	294:306	carcass yield	294:306	Two separate experiments were conducted to evaluate the efficacy of either prebiotic or synbiotic on growth performance, carcass yield, organ weights, and serum metabolic profile of broilers under hot climatic conditions.
30198753	8	10	theme	22-42 d	1017:1023	arg1	ratio					1079:1083	feed conversion ratio	1063:1083	feed conversion ratio (FCR)	1063:1089	Supplementation of synbiotic at 1 or 1.5 g kg-1 feed to broiler diets during 1-21 d and 0.5 or 0.75 g kg-1 feed, respectively, during 22-42 d improved (P < 0.01) body weight gain, feed conversion ratio (FCR), and decreased (P < 0.01) abdominal fat, serum total cholesterol, and low-density lipoprotein (LDL) cholesterol levels in experiment 1.
30198753	8	10	theme	22-42 d	1017:1023	arg1	gain					1057:1060	22-42 d improved (P < 0.01) body weight gain	1017:1060	22-42 d improved (P < 0.01) body weight gain	1017:1060	Supplementation of synbiotic at 1 or 1.5 g kg-1 feed to broiler diets during 1-21 d and 0.5 or 0.75 g kg-1 feed, respectively, during 22-42 d improved (P < 0.01) body weight gain, feed conversion ratio (FCR), and decreased (P < 0.01) abdominal fat, serum total cholesterol, and low-density lipoprotein (LDL) cholesterol levels in experiment 1.
30198753	4	11	theme	1-d-old	419:425	arg1	chickens					449:456	150 1-d-old Ross 308 male broiler chickens	415:456	150 1-d-old Ross 308 male broiler chickens	415:456	In experiment 1, 150 1-d-old Ross 308 male broiler chickens were fed dietary synbiotic (Biomin® IMBO, 0, 1 or 1.5 g kg-1 of the starter diets and 0, 0.5 or 0.75 g kg-1 of the grower diets) for 6 weeks.
30198753	8	12	theme	conversion	1068:1077	arg1	FCR					1086:1088	FCR	1086:1088	FCR	1086:1088	Supplementation of synbiotic at 1 or 1.5 g kg-1 feed to broiler diets during 1-21 d and 0.5 or 0.75 g kg-1 feed, respectively, during 22-42 d improved (P < 0.01) body weight gain, feed conversion ratio (FCR), and decreased (P < 0.01) abdominal fat, serum total cholesterol, and low-density lipoprotein (LDL) cholesterol levels in experiment 1.
30198753	8	12	theme	conversion	1068:1077	arg1	ratio					1079:1083	feed conversion ratio	1063:1083	feed conversion ratio (FCR)	1063:1089	Supplementation of synbiotic at 1 or 1.5 g kg-1 feed to broiler diets during 1-21 d and 0.5 or 0.75 g kg-1 feed, respectively, during 22-42 d improved (P < 0.01) body weight gain, feed conversion ratio (FCR), and decreased (P < 0.01) abdominal fat, serum total cholesterol, and low-density lipoprotein (LDL) cholesterol levels in experiment 1.
30198753	8	12	theme	conversion	1068:1077	arg1	gain					1057:1060	22-42 d improved (P < 0.01) body weight gain	1017:1060	22-42 d improved (P < 0.01) body weight gain	1017:1060	Supplementation of synbiotic at 1 or 1.5 g kg-1 feed to broiler diets during 1-21 d and 0.5 or 0.75 g kg-1 feed, respectively, during 22-42 d improved (P < 0.01) body weight gain, feed conversion ratio (FCR), and decreased (P < 0.01) abdominal fat, serum total cholesterol, and low-density lipoprotein (LDL) cholesterol levels in experiment 1.
30198753	5	13	theme	kg-1	725:728	arg1	diets					791:795	Mannan oligosaccharide 0, 0.5 or 1 g kg-1 of the starter diets and 0, 0.25 or 0.5 g kg-1 of the grower diets	688:795	Mannan oligosaccharide 0, 0.5 or 1 g kg-1 of the starter diets and 0, 0.25 or 0.5 g kg-1 of the grower diets	688:795	In experiment 2, 150 1-d-old Ross 308 male broiler chickens were fed dietary prebiotic (Mannan oligosaccharide 0, 0.5 or 1 g kg-1 of the starter diets and 0, 0.25 or 0.5 g kg-1 of the grower diets) for 6 weeks.
30198753	5	13	theme	kg-1	725:728	arg1	prebiotic					677:685	prebiotic	677:685	prebiotic	677:685	In experiment 2, 150 1-d-old Ross 308 male broiler chickens were fed dietary prebiotic (Mannan oligosaccharide 0, 0.5 or 1 g kg-1 of the starter diets and 0, 0.25 or 0.5 g kg-1 of the grower diets) for 6 weeks.
30198753	0	14	theme	hot	145:147	arg1	conditions					158:167	hot climatic conditions	145:167	hot climatic conditions	145:167	Growth performance, carcass criteria, and serum biochemical parameters of broiler chickens supplemented with either synbiotic or prebiotic under hot climatic conditions.
30198753	10	15	theme	LDL-cholesterol	1489:1503	arg1	levels					1505:1510	LDL-cholesterol levels	1489:1510	LDL-cholesterol levels in experiment 2	1489:1526	Similarly, supplementation of the prebiotic at 0.5 or 1 g kg-1 in broiler diets during 1-21 d and 0.25 or 0.50 g kg-1 feed, respectively, during 22-42 d improved (P < 0.01) body weight gain, FCR, and decreased (P < 0.01) abdominal fat, serum cholesterol, and LDL-cholesterol levels in experiment 2.
30198753	5	16	theme	broiler	643:649	arg1	chickens					651:658	150 1-d-old Ross 308 male broiler chickens	617:658	150 1-d-old Ross 308 male broiler chickens	617:658	In experiment 2, 150 1-d-old Ross 308 male broiler chickens were fed dietary prebiotic (Mannan oligosaccharide 0, 0.5 or 1 g kg-1 of the starter diets and 0, 0.25 or 0.5 g kg-1 of the grower diets) for 6 weeks.
30198753	10	17	from	supplementation	1241:1255	arg1	kg-1					1288:1291	0.5 or 1 g kg-1	1277:1291	0.5 or 1 g kg-1	1277:1291	Similarly, supplementation of the prebiotic at 0.5 or 1 g kg-1 in broiler diets during 1-21 d and 0.25 or 0.50 g kg-1 feed, respectively, during 22-42 d improved (P < 0.01) body weight gain, FCR, and decreased (P < 0.01) abdominal fat, serum cholesterol, and LDL-cholesterol levels in experiment 2.
30198753	10	17	from	supplementation	1241:1255	arg1	diets					1304:1308	broiler diets	1296:1308	broiler diets	1296:1308	Similarly, supplementation of the prebiotic at 0.5 or 1 g kg-1 in broiler diets during 1-21 d and 0.25 or 0.50 g kg-1 feed, respectively, during 22-42 d improved (P < 0.01) body weight gain, FCR, and decreased (P < 0.01) abdominal fat, serum cholesterol, and LDL-cholesterol levels in experiment 2.
30198753	10	18	from	cholesterol	1472:1482	arg1	experiment					1515:1524	experiment 2	1515:1526	experiment 2	1515:1526	Similarly, supplementation of the prebiotic at 0.5 or 1 g kg-1 in broiler diets during 1-21 d and 0.25 or 0.50 g kg-1 feed, respectively, during 22-42 d improved (P < 0.01) body weight gain, FCR, and decreased (P < 0.01) abdominal fat, serum cholesterol, and LDL-cholesterol levels in experiment 2.
30198753	4	19	theme	dietary	467:473	arg1	synbiotic					475:483	dietary synbiotic	467:483	dietary synbiotic (Biomin® IMBO, 0, 1 or 1.5 g kg-1 of the starter diets and 0, 0.5 or 0.75 g kg-1 of the grower diets)	467:585	In experiment 1, 150 1-d-old Ross 308 male broiler chickens were fed dietary synbiotic (Biomin® IMBO, 0, 1 or 1.5 g kg-1 of the starter diets and 0, 0.5 or 0.75 g kg-1 of the grower diets) for 6 weeks.
30198753	8	20	from	levels	1203:1208	arg1	experiment					1213:1222	experiment 1	1213:1224	experiment 1	1213:1224	Supplementation of synbiotic at 1 or 1.5 g kg-1 feed to broiler diets during 1-21 d and 0.5 or 0.75 g kg-1 feed, respectively, during 22-42 d improved (P < 0.01) body weight gain, feed conversion ratio (FCR), and decreased (P < 0.01) abdominal fat, serum total cholesterol, and low-density lipoprotein (LDL) cholesterol levels in experiment 1.
30198753	10	21	from	prebiotic	1264:1272	arg1	diets					1304:1308	broiler diets	1296:1308	broiler diets	1296:1308	Similarly, supplementation of the prebiotic at 0.5 or 1 g kg-1 in broiler diets during 1-21 d and 0.25 or 0.50 g kg-1 feed, respectively, during 22-42 d improved (P < 0.01) body weight gain, FCR, and decreased (P < 0.01) abdominal fat, serum cholesterol, and LDL-cholesterol levels in experiment 2.
30198753	0	22	theme	chickens	82:89	arg1	performance					7:17	Growth performance	0:17	Growth performance	0:17	Growth performance, carcass criteria, and serum biochemical parameters of broiler chickens supplemented with either synbiotic or prebiotic under hot climatic conditions.
30198753	0	22	theme	chickens	82:89	arg1	criteria					28:35	carcass criteria	20:35	carcass criteria	20:35	Growth performance, carcass criteria, and serum biochemical parameters of broiler chickens supplemented with either synbiotic or prebiotic under hot climatic conditions.
30198753	0	22	theme	chickens	82:89	arg1	parameters					60:69	serum biochemical parameters	42:69	serum biochemical parameters of broiler chickens	42:89	Growth performance, carcass criteria, and serum biochemical parameters of broiler chickens supplemented with either synbiotic or prebiotic under hot climatic conditions.
30198753	10	23	from	levels	1505:1510	arg1	experiment					1515:1524	experiment 2	1515:1526	experiment 2	1515:1526	Similarly, supplementation of the prebiotic at 0.5 or 1 g kg-1 in broiler diets during 1-21 d and 0.25 or 0.50 g kg-1 feed, respectively, during 22-42 d improved (P < 0.01) body weight gain, FCR, and decreased (P < 0.01) abdominal fat, serum cholesterol, and LDL-cholesterol levels in experiment 2.
30198753	8	24	from	kg-1	926:929	arg1	synbiotic					902:910	synbiotic	902:910	synbiotic	902:910	Supplementation of synbiotic at 1 or 1.5 g kg-1 feed to broiler diets during 1-21 d and 0.5 or 0.75 g kg-1 feed, respectively, during 22-42 d improved (P < 0.01) body weight gain, feed conversion ratio (FCR), and decreased (P < 0.01) abdominal fat, serum total cholesterol, and low-density lipoprotein (LDL) cholesterol levels in experiment 1.
30198753	4	25	theme	broiler	441:447	arg1	chickens					449:456	150 1-d-old Ross 308 male broiler chickens	415:456	150 1-d-old Ross 308 male broiler chickens	415:456	In experiment 1, 150 1-d-old Ross 308 male broiler chickens were fed dietary synbiotic (Biomin® IMBO, 0, 1 or 1.5 g kg-1 of the starter diets and 0, 0.5 or 0.75 g kg-1 of the grower diets) for 6 weeks.
30198753	8	26	theme	serum	1132:1136	arg1	cholesterol					1144:1154	serum total cholesterol	1132:1154	serum total cholesterol	1132:1154	Supplementation of synbiotic at 1 or 1.5 g kg-1 feed to broiler diets during 1-21 d and 0.5 or 0.75 g kg-1 feed, respectively, during 22-42 d improved (P < 0.01) body weight gain, feed conversion ratio (FCR), and decreased (P < 0.01) abdominal fat, serum total cholesterol, and low-density lipoprotein (LDL) cholesterol levels in experiment 1.
30198753	2	27	theme	hot	370:372	arg1	conditions					383:392	hot climatic conditions	370:392	hot climatic conditions	370:392	Two separate experiments were conducted to evaluate the efficacy of either prebiotic or synbiotic on growth performance, carcass yield, organ weights, and serum metabolic profile of broilers under hot climatic conditions.
30198753	5	28	theme	oligosaccharide	695:709	arg1	diets					791:795	Mannan oligosaccharide 0, 0.5 or 1 g kg-1 of the starter diets and 0, 0.25 or 0.5 g kg-1 of the grower diets	688:795	Mannan oligosaccharide 0, 0.5 or 1 g kg-1 of the starter diets and 0, 0.25 or 0.5 g kg-1 of the grower diets	688:795	In experiment 2, 150 1-d-old Ross 308 male broiler chickens were fed dietary prebiotic (Mannan oligosaccharide 0, 0.5 or 1 g kg-1 of the starter diets and 0, 0.25 or 0.5 g kg-1 of the grower diets) for 6 weeks.
30198753	5	28	theme	oligosaccharide	695:709	arg1	prebiotic					677:685	prebiotic	677:685	prebiotic	677:685	In experiment 2, 150 1-d-old Ross 308 male broiler chickens were fed dietary prebiotic (Mannan oligosaccharide 0, 0.5 or 1 g kg-1 of the starter diets and 0, 0.25 or 0.5 g kg-1 of the grower diets) for 6 weeks.
30198753	5	29	theme	grower	784:789	arg1	kg-1					725:728	0, 0.5 or 1 g kg-1	711:728	0, 0.5 or 1 g kg-1 of the starter diets	711:749	In experiment 2, 150 1-d-old Ross 308 male broiler chickens were fed dietary prebiotic (Mannan oligosaccharide 0, 0.5 or 1 g kg-1 of the starter diets and 0, 0.25 or 0.5 g kg-1 of the grower diets) for 6 weeks.
30198753	5	29	theme	grower	784:789	arg1	kg-1					772:775	0, 0.25 or 0.5 g kg-1	755:775	0, 0.25 or 0.5 g kg-1 of the grower	755:789	In experiment 2, 150 1-d-old Ross 308 male broiler chickens were fed dietary prebiotic (Mannan oligosaccharide 0, 0.5 or 1 g kg-1 of the starter diets and 0, 0.25 or 0.5 g kg-1 of the grower diets) for 6 weeks.
30198753	6	30	contain	had	826:828	arg2	10					830:831	10	830:831	10	830:831	Each treatment had 10 replicates containing 5 broiler chickens each.
30198753	6	30	contain	had	826:828	arg1	treatment					816:824	Each treatment	811:824	Each treatment	811:824	Each treatment had 10 replicates containing 5 broiler chickens each.
30198753	10	31	theme	weight	1408:1413	arg1	gain					1415:1418	22-42 d improved (P < 0.01) body weight gain	1375:1418	22-42 d improved (P < 0.01) body weight gain	1375:1418	Similarly, supplementation of the prebiotic at 0.5 or 1 g kg-1 in broiler diets during 1-21 d and 0.25 or 0.50 g kg-1 feed, respectively, during 22-42 d improved (P < 0.01) body weight gain, FCR, and decreased (P < 0.01) abdominal fat, serum cholesterol, and LDL-cholesterol levels in experiment 2.
30198753	10	32	theme	abdominal	1451:1459	arg1	fat					1461:1463	(P < 0.01) abdominal fat	1440:1463	(P < 0.01) abdominal fat	1440:1463	Similarly, supplementation of the prebiotic at 0.5 or 1 g kg-1 in broiler diets during 1-21 d and 0.25 or 0.50 g kg-1 feed, respectively, during 22-42 d improved (P < 0.01) body weight gain, FCR, and decreased (P < 0.01) abdominal fat, serum cholesterol, and LDL-cholesterol levels in experiment 2.
30198753	10	33	from	kg-1	1288:1291	arg1	supplementation					1241:1255	supplementation	1241:1255	supplementation of the prebiotic at 0.5 or 1 g kg-1 in broiler diets during 1-21 d and 0.25 or 0.50 g kg-1	1241:1346	Similarly, supplementation of the prebiotic at 0.5 or 1 g kg-1 in broiler diets during 1-21 d and 0.25 or 0.50 g kg-1 feed, respectively, during 22-42 d improved (P < 0.01) body weight gain, FCR, and decreased (P < 0.01) abdominal fat, serum cholesterol, and LDL-cholesterol levels in experiment 2.
30198753	10	33	from	kg-1	1288:1291	arg1	prebiotic					1264:1272	the prebiotic	1260:1272	the prebiotic at 0.5 or 1 g kg-1 in broiler diets	1260:1308	Similarly, supplementation of the prebiotic at 0.5 or 1 g kg-1 in broiler diets during 1-21 d and 0.25 or 0.50 g kg-1 feed, respectively, during 22-42 d improved (P < 0.01) body weight gain, FCR, and decreased (P < 0.01) abdominal fat, serum cholesterol, and LDL-cholesterol levels in experiment 2.
30198753	0	34	theme	biochemical	48:58	arg1	parameters					60:69	serum biochemical parameters	42:69	serum biochemical parameters of broiler chickens	42:89	Growth performance, carcass criteria, and serum biochemical parameters of broiler chickens supplemented with either synbiotic or prebiotic under hot climatic conditions.
30198753	8	35	theme	lipoprotein	1173:1183	arg1	levels					1203:1208	low-density lipoprotein (LDL) cholesterol levels	1161:1208	low-density lipoprotein (LDL) cholesterol levels	1161:1208	Supplementation of synbiotic at 1 or 1.5 g kg-1 feed to broiler diets during 1-21 d and 0.5 or 0.75 g kg-1 feed, respectively, during 22-42 d improved (P < 0.01) body weight gain, feed conversion ratio (FCR), and decreased (P < 0.01) abdominal fat, serum total cholesterol, and low-density lipoprotein (LDL) cholesterol levels in experiment 1.
30198753	2	36	theme	serum	328:332	arg1	profile					344:350	serum metabolic profile	328:350	serum metabolic profile	328:350	Two separate experiments were conducted to evaluate the efficacy of either prebiotic or synbiotic on growth performance, carcass yield, organ weights, and serum metabolic profile of broilers under hot climatic conditions.
30198753	4	37	dep	synbiotic	475:483	arg1	IMBO					494:497	IMBO	494:497	IMBO	494:497	In experiment 1, 150 1-d-old Ross 308 male broiler chickens were fed dietary synbiotic (Biomin® IMBO, 0, 1 or 1.5 g kg-1 of the starter diets and 0, 0.5 or 0.75 g kg-1 of the grower diets) for 6 weeks.
30198753	8	38	theme	LDL	1186:1188	arg1	levels					1203:1208	low-density lipoprotein (LDL) cholesterol levels	1161:1208	low-density lipoprotein (LDL) cholesterol levels	1161:1208	Supplementation of synbiotic at 1 or 1.5 g kg-1 feed to broiler diets during 1-21 d and 0.5 or 0.75 g kg-1 feed, respectively, during 22-42 d improved (P < 0.01) body weight gain, feed conversion ratio (FCR), and decreased (P < 0.01) abdominal fat, serum total cholesterol, and low-density lipoprotein (LDL) cholesterol levels in experiment 1.
30198753	10	39	theme	broiler	1296:1302	arg1	diets					1304:1308	broiler diets	1296:1308	broiler diets	1296:1308	Similarly, supplementation of the prebiotic at 0.5 or 1 g kg-1 in broiler diets during 1-21 d and 0.25 or 0.50 g kg-1 feed, respectively, during 22-42 d improved (P < 0.01) body weight gain, FCR, and decreased (P < 0.01) abdominal fat, serum cholesterol, and LDL-cholesterol levels in experiment 2.
30198753	8	40	theme	cholesterol	1191:1201	arg1	levels					1203:1208	low-density lipoprotein (LDL) cholesterol levels	1161:1208	low-density lipoprotein (LDL) cholesterol levels	1161:1208	Supplementation of synbiotic at 1 or 1.5 g kg-1 feed to broiler diets during 1-21 d and 0.5 or 0.75 g kg-1 feed, respectively, during 22-42 d improved (P < 0.01) body weight gain, feed conversion ratio (FCR), and decreased (P < 0.01) abdominal fat, serum total cholesterol, and low-density lipoprotein (LDL) cholesterol levels in experiment 1.
30198753	10	41	theme	P < 0.01	1441:1448	arg1	fat					1461:1463	(P < 0.01) abdominal fat	1440:1463	(P < 0.01) abdominal fat	1440:1463	Similarly, supplementation of the prebiotic at 0.5 or 1 g kg-1 in broiler diets during 1-21 d and 0.25 or 0.50 g kg-1 feed, respectively, during 22-42 d improved (P < 0.01) body weight gain, FCR, and decreased (P < 0.01) abdominal fat, serum cholesterol, and LDL-cholesterol levels in experiment 2.
30198753	10	42	theme	decreased	1430:1438	arg1	fat					1461:1463	(P < 0.01) abdominal fat	1440:1463	(P < 0.01) abdominal fat	1440:1463	Similarly, supplementation of the prebiotic at 0.5 or 1 g kg-1 in broiler diets during 1-21 d and 0.25 or 0.50 g kg-1 feed, respectively, during 22-42 d improved (P < 0.01) body weight gain, FCR, and decreased (P < 0.01) abdominal fat, serum cholesterol, and LDL-cholesterol levels in experiment 2.
30198753	4	43	theme	starter	526:532	arg1	diets					534:538	the starter diets	522:538	the starter diets	522:538	In experiment 1, 150 1-d-old Ross 308 male broiler chickens were fed dietary synbiotic (Biomin® IMBO, 0, 1 or 1.5 g kg-1 of the starter diets and 0, 0.5 or 0.75 g kg-1 of the grower diets) for 6 weeks.
30198753	2	44	theme	growth	274:279	arg1	performance					281:291	growth performance	274:291	growth performance	274:291	Two separate experiments were conducted to evaluate the efficacy of either prebiotic or synbiotic on growth performance, carcass yield, organ weights, and serum metabolic profile of broilers under hot climatic conditions.
30198753	8	45	theme	P < 0.01	1035:1042	arg1	ratio					1079:1083	feed conversion ratio	1063:1083	feed conversion ratio (FCR)	1063:1089	Supplementation of synbiotic at 1 or 1.5 g kg-1 feed to broiler diets during 1-21 d and 0.5 or 0.75 g kg-1 feed, respectively, during 22-42 d improved (P < 0.01) body weight gain, feed conversion ratio (FCR), and decreased (P < 0.01) abdominal fat, serum total cholesterol, and low-density lipoprotein (LDL) cholesterol levels in experiment 1.
30198753	8	45	theme	P < 0.01	1035:1042	arg1	gain					1057:1060	22-42 d improved (P < 0.01) body weight gain	1017:1060	22-42 d improved (P < 0.01) body weight gain	1017:1060	Supplementation of synbiotic at 1 or 1.5 g kg-1 feed to broiler diets during 1-21 d and 0.5 or 0.75 g kg-1 feed, respectively, during 22-42 d improved (P < 0.01) body weight gain, feed conversion ratio (FCR), and decreased (P < 0.01) abdominal fat, serum total cholesterol, and low-density lipoprotein (LDL) cholesterol levels in experiment 1.
30198753	2	46	theme	organ	309:313	arg1	weights					315:321	organ weights	309:321	organ weights	309:321	Two separate experiments were conducted to evaluate the efficacy of either prebiotic or synbiotic on growth performance, carcass yield, organ weights, and serum metabolic profile of broilers under hot climatic conditions.
30198753	5	47	theme	starter	737:743	arg1	diets					745:749	the starter diets	733:749	the starter diets	733:749	In experiment 2, 150 1-d-old Ross 308 male broiler chickens were fed dietary prebiotic (Mannan oligosaccharide 0, 0.5 or 1 g kg-1 of the starter diets and 0, 0.25 or 0.5 g kg-1 of the grower diets) for 6 weeks.
30198753	4	48	theme	diets	580:584	arg1	kg-1					561:564	0, 0.5 or 0.75 g kg-1	544:564	0, 0.5 or 0.75 g kg-1 of the grower diets	544:584	In experiment 1, 150 1-d-old Ross 308 male broiler chickens were fed dietary synbiotic (Biomin® IMBO, 0, 1 or 1.5 g kg-1 of the starter diets and 0, 0.5 or 0.75 g kg-1 of the grower diets) for 6 weeks.
30198753	4	48	theme	diets	580:584	arg1	kg-1					514:517	0, 1 or 1.5 g kg-1	500:517	0, 1 or 1.5 g kg-1 of the starter diets	500:538	In experiment 1, 150 1-d-old Ross 308 male broiler chickens were fed dietary synbiotic (Biomin® IMBO, 0, 1 or 1.5 g kg-1 of the starter diets and 0, 0.5 or 0.75 g kg-1 of the grower diets) for 6 weeks.
30198753	2	49	theme	broilers	355:362	arg1	performance					281:291	growth performance	274:291	growth performance	274:291	Two separate experiments were conducted to evaluate the efficacy of either prebiotic or synbiotic on growth performance, carcass yield, organ weights, and serum metabolic profile of broilers under hot climatic conditions.
30198753	2	49	theme	broilers	355:362	arg1	profile					344:350	serum metabolic profile	328:350	serum metabolic profile	328:350	Two separate experiments were conducted to evaluate the efficacy of either prebiotic or synbiotic on growth performance, carcass yield, organ weights, and serum metabolic profile of broilers under hot climatic conditions.
30198753	2	49	theme	broilers	355:362	arg1	weights					315:321	organ weights	309:321	organ weights	309:321	Two separate experiments were conducted to evaluate the efficacy of either prebiotic or synbiotic on growth performance, carcass yield, organ weights, and serum metabolic profile of broilers under hot climatic conditions.
30198753	2	49	theme	broilers	355:362	arg1	yield					302:306	carcass yield	294:306	carcass yield	294:306	Two separate experiments were conducted to evaluate the efficacy of either prebiotic or synbiotic on growth performance, carcass yield, organ weights, and serum metabolic profile of broilers under hot climatic conditions.
30198753	4	50	theme	Ross	427:430	arg1	chickens					449:456	150 1-d-old Ross 308 male broiler chickens	415:456	150 1-d-old Ross 308 male broiler chickens	415:456	In experiment 1, 150 1-d-old Ross 308 male broiler chickens were fed dietary synbiotic (Biomin® IMBO, 0, 1 or 1.5 g kg-1 of the starter diets and 0, 0.5 or 0.75 g kg-1 of the grower diets) for 6 weeks.
30198753	8	51	theme	body	1045:1048	arg1	ratio					1079:1083	feed conversion ratio	1063:1083	feed conversion ratio (FCR)	1063:1089	Supplementation of synbiotic at 1 or 1.5 g kg-1 feed to broiler diets during 1-21 d and 0.5 or 0.75 g kg-1 feed, respectively, during 22-42 d improved (P < 0.01) body weight gain, feed conversion ratio (FCR), and decreased (P < 0.01) abdominal fat, serum total cholesterol, and low-density lipoprotein (LDL) cholesterol levels in experiment 1.
30198753	8	51	theme	body	1045:1048	arg1	gain					1057:1060	22-42 d improved (P < 0.01) body weight gain	1017:1060	22-42 d improved (P < 0.01) body weight gain	1017:1060	Supplementation of synbiotic at 1 or 1.5 g kg-1 feed to broiler diets during 1-21 d and 0.5 or 0.75 g kg-1 feed, respectively, during 22-42 d improved (P < 0.01) body weight gain, feed conversion ratio (FCR), and decreased (P < 0.01) abdominal fat, serum total cholesterol, and low-density lipoprotein (LDL) cholesterol levels in experiment 1.
30198753	4	52	theme	male	436:439	arg1	chickens					449:456	150 1-d-old Ross 308 male broiler chickens	415:456	150 1-d-old Ross 308 male broiler chickens	415:456	In experiment 1, 150 1-d-old Ross 308 male broiler chickens were fed dietary synbiotic (Biomin® IMBO, 0, 1 or 1.5 g kg-1 of the starter diets and 0, 0.5 or 0.75 g kg-1 of the grower diets) for 6 weeks.
30198753	8	53	theme	feed	1063:1066	arg1	FCR					1086:1088	FCR	1086:1088	FCR	1086:1088	Supplementation of synbiotic at 1 or 1.5 g kg-1 feed to broiler diets during 1-21 d and 0.5 or 0.75 g kg-1 feed, respectively, during 22-42 d improved (P < 0.01) body weight gain, feed conversion ratio (FCR), and decreased (P < 0.01) abdominal fat, serum total cholesterol, and low-density lipoprotein (LDL) cholesterol levels in experiment 1.
30198753	8	53	theme	feed	1063:1066	arg1	ratio					1079:1083	feed conversion ratio	1063:1083	feed conversion ratio (FCR)	1063:1089	Supplementation of synbiotic at 1 or 1.5 g kg-1 feed to broiler diets during 1-21 d and 0.5 or 0.75 g kg-1 feed, respectively, during 22-42 d improved (P < 0.01) body weight gain, feed conversion ratio (FCR), and decreased (P < 0.01) abdominal fat, serum total cholesterol, and low-density lipoprotein (LDL) cholesterol levels in experiment 1.
30198753	8	53	theme	feed	1063:1066	arg1	gain					1057:1060	22-42 d improved (P < 0.01) body weight gain	1017:1060	22-42 d improved (P < 0.01) body weight gain	1017:1060	Supplementation of synbiotic at 1 or 1.5 g kg-1 feed to broiler diets during 1-21 d and 0.5 or 0.75 g kg-1 feed, respectively, during 22-42 d improved (P < 0.01) body weight gain, feed conversion ratio (FCR), and decreased (P < 0.01) abdominal fat, serum total cholesterol, and low-density lipoprotein (LDL) cholesterol levels in experiment 1.
30198753	0	54	theme	climatic	149:156	arg1	conditions					158:167	hot climatic conditions	145:167	hot climatic conditions	145:167	Growth performance, carcass criteria, and serum biochemical parameters of broiler chickens supplemented with either synbiotic or prebiotic under hot climatic conditions.
30198753	8	55	theme	improved	1025:1032	arg1	ratio					1079:1083	feed conversion ratio	1063:1083	feed conversion ratio (FCR)	1063:1089	Supplementation of synbiotic at 1 or 1.5 g kg-1 feed to broiler diets during 1-21 d and 0.5 or 0.75 g kg-1 feed, respectively, during 22-42 d improved (P < 0.01) body weight gain, feed conversion ratio (FCR), and decreased (P < 0.01) abdominal fat, serum total cholesterol, and low-density lipoprotein (LDL) cholesterol levels in experiment 1.
30198753	8	55	theme	improved	1025:1032	arg1	gain					1057:1060	22-42 d improved (P < 0.01) body weight gain	1017:1060	22-42 d improved (P < 0.01) body weight gain	1017:1060	Supplementation of synbiotic at 1 or 1.5 g kg-1 feed to broiler diets during 1-21 d and 0.5 or 0.75 g kg-1 feed, respectively, during 22-42 d improved (P < 0.01) body weight gain, feed conversion ratio (FCR), and decreased (P < 0.01) abdominal fat, serum total cholesterol, and low-density lipoprotein (LDL) cholesterol levels in experiment 1.
30198753	2	56	theme	synbiotic	261:269	arg1	efficacy					229:236	the efficacy	225:236	the efficacy of either prebiotic or synbiotic on growth performance, carcass yield, organ weights, and serum metabolic profile of broilers	225:362	Two separate experiments were conducted to evaluate the efficacy of either prebiotic or synbiotic on growth performance, carcass yield, organ weights, and serum metabolic profile of broilers under hot climatic conditions.
30198753	5	57	theme	kg-1	772:775	arg1	diets					791:795	Mannan oligosaccharide 0, 0.5 or 1 g kg-1 of the starter diets and 0, 0.25 or 0.5 g kg-1 of the grower diets	688:795	Mannan oligosaccharide 0, 0.5 or 1 g kg-1 of the starter diets and 0, 0.25 or 0.5 g kg-1 of the grower diets	688:795	In experiment 2, 150 1-d-old Ross 308 male broiler chickens were fed dietary prebiotic (Mannan oligosaccharide 0, 0.5 or 1 g kg-1 of the starter diets and 0, 0.25 or 0.5 g kg-1 of the grower diets) for 6 weeks.
30198753	5	57	theme	kg-1	772:775	arg1	prebiotic					677:685	prebiotic	677:685	prebiotic	677:685	In experiment 2, 150 1-d-old Ross 308 male broiler chickens were fed dietary prebiotic (Mannan oligosaccharide 0, 0.5 or 1 g kg-1 of the starter diets and 0, 0.25 or 0.5 g kg-1 of the grower diets) for 6 weeks.
30198753	6	58	theme	broiler	857:863	arg1	chickens					865:872	5 broiler chickens	855:872	5 broiler chickens each	855:877	Each treatment had 10 replicates containing 5 broiler chickens each.
30198753	5	59	theme	male	638:641	arg1	chickens					651:658	150 1-d-old Ross 308 male broiler chickens	617:658	150 1-d-old Ross 308 male broiler chickens	617:658	In experiment 2, 150 1-d-old Ross 308 male broiler chickens were fed dietary prebiotic (Mannan oligosaccharide 0, 0.5 or 1 g kg-1 of the starter diets and 0, 0.25 or 0.5 g kg-1 of the grower diets) for 6 weeks.
30198753	2	60	theme	prebiotic	248:256	arg1	efficacy					229:236	the efficacy	225:236	the efficacy of either prebiotic or synbiotic on growth performance, carcass yield, organ weights, and serum metabolic profile of broilers	225:362	Two separate experiments were conducted to evaluate the efficacy of either prebiotic or synbiotic on growth performance, carcass yield, organ weights, and serum metabolic profile of broilers under hot climatic conditions.
30198753	10	61	from	fat	1461:1463	arg1	experiment					1515:1524	experiment 2	1515:1526	experiment 2	1515:1526	Similarly, supplementation of the prebiotic at 0.5 or 1 g kg-1 in broiler diets during 1-21 d and 0.25 or 0.50 g kg-1 feed, respectively, during 22-42 d improved (P < 0.01) body weight gain, FCR, and decreased (P < 0.01) abdominal fat, serum cholesterol, and LDL-cholesterol levels in experiment 2.
30198753	5	62	theme	1-d-old	621:627	arg1	chickens					651:658	150 1-d-old Ross 308 male broiler chickens	617:658	150 1-d-old Ross 308 male broiler chickens	617:658	In experiment 2, 150 1-d-old Ross 308 male broiler chickens were fed dietary prebiotic (Mannan oligosaccharide 0, 0.5 or 1 g kg-1 of the starter diets and 0, 0.25 or 0.5 g kg-1 of the grower diets) for 6 weeks.
30198753	0	63	theme	broiler	74:80	arg1	chickens					82:89	broiler chickens	74:89	broiler chickens	74:89	Growth performance, carcass criteria, and serum biochemical parameters of broiler chickens supplemented with either synbiotic or prebiotic under hot climatic conditions.
30198753	6	64	dep	10	830:831	arg1	replicates					833:842	replicates	833:842	replicates	833:842	Each treatment had 10 replicates containing 5 broiler chickens each.
30198753	4	65	dep	IMBO	494:497	arg1	kg-1					561:564	0, 0.5 or 0.75 g kg-1	544:564	0, 0.5 or 0.75 g kg-1 of the grower diets	544:584	In experiment 1, 150 1-d-old Ross 308 male broiler chickens were fed dietary synbiotic (Biomin® IMBO, 0, 1 or 1.5 g kg-1 of the starter diets and 0, 0.5 or 0.75 g kg-1 of the grower diets) for 6 weeks.
30198753	4	65	dep	IMBO	494:497	arg1	kg-1					514:517	0, 1 or 1.5 g kg-1	500:517	0, 1 or 1.5 g kg-1 of the starter diets	500:538	In experiment 1, 150 1-d-old Ross 308 male broiler chickens were fed dietary synbiotic (Biomin® IMBO, 0, 1 or 1.5 g kg-1 of the starter diets and 0, 0.5 or 0.75 g kg-1 of the grower diets) for 6 weeks.
30198753	10	66	theme	serum	1466:1470	arg1	cholesterol					1472:1482	serum cholesterol	1466:1482	serum cholesterol	1466:1482	Similarly, supplementation of the prebiotic at 0.5 or 1 g kg-1 in broiler diets during 1-21 d and 0.25 or 0.50 g kg-1 feed, respectively, during 22-42 d improved (P < 0.01) body weight gain, FCR, and decreased (P < 0.01) abdominal fat, serum cholesterol, and LDL-cholesterol levels in experiment 2.
30198753	6	67	contain	containing	844:853	arg2	chickens					865:872	5 broiler chickens	855:872	5 broiler chickens each	855:877	Each treatment had 10 replicates containing 5 broiler chickens each.
30198753	6	67	contain	containing	844:853	arg1	10					830:831	10	830:831	10	830:831	Each treatment had 10 replicates containing 5 broiler chickens each.
30198753	6	68	dep	chickens	865:872	arg1	each					874:877	each	874:877	each	874:877	Each treatment had 10 replicates containing 5 broiler chickens each.
30198753	5	69	theme	Ross	629:632	arg1	chickens					651:658	150 1-d-old Ross 308 male broiler chickens	617:658	150 1-d-old Ross 308 male broiler chickens	617:658	In experiment 2, 150 1-d-old Ross 308 male broiler chickens were fed dietary prebiotic (Mannan oligosaccharide 0, 0.5 or 1 g kg-1 of the starter diets and 0, 0.25 or 0.5 g kg-1 of the grower diets) for 6 weeks.
30198753	8	70	theme	abdominal	1117:1125	arg1	fat					1127:1129	(P < 0.01) abdominal fat	1106:1129	(P < 0.01) abdominal fat	1106:1129	Supplementation of synbiotic at 1 or 1.5 g kg-1 feed to broiler diets during 1-21 d and 0.5 or 0.75 g kg-1 feed, respectively, during 22-42 d improved (P < 0.01) body weight gain, feed conversion ratio (FCR), and decreased (P < 0.01) abdominal fat, serum total cholesterol, and low-density lipoprotein (LDL) cholesterol levels in experiment 1.
30198753	2	71	theme	climatic	374:381	arg1	conditions					383:392	hot climatic conditions	370:392	hot climatic conditions	370:392	Two separate experiments were conducted to evaluate the efficacy of either prebiotic or synbiotic on growth performance, carcass yield, organ weights, and serum metabolic profile of broilers under hot climatic conditions.
30198753	5	72	theme	Mannan	688:693	arg1	diets					791:795	Mannan oligosaccharide 0, 0.5 or 1 g kg-1 of the starter diets and 0, 0.25 or 0.5 g kg-1 of the grower diets	688:795	Mannan oligosaccharide 0, 0.5 or 1 g kg-1 of the starter diets and 0, 0.25 or 0.5 g kg-1 of the grower diets	688:795	In experiment 2, 150 1-d-old Ross 308 male broiler chickens were fed dietary prebiotic (Mannan oligosaccharide 0, 0.5 or 1 g kg-1 of the starter diets and 0, 0.25 or 0.5 g kg-1 of the grower diets) for 6 weeks.
30198753	5	72	theme	Mannan	688:693	arg1	prebiotic					677:685	prebiotic	677:685	prebiotic	677:685	In experiment 2, 150 1-d-old Ross 308 male broiler chickens were fed dietary prebiotic (Mannan oligosaccharide 0, 0.5 or 1 g kg-1 of the starter diets and 0, 0.25 or 0.5 g kg-1 of the grower diets) for 6 weeks.
30198753	8	73	theme	synbiotic	902:910	arg1	feed					931:934	synbiotic at 1 or 1.5 g kg-1 feed	902:934	synbiotic at 1 or 1.5 g kg-1 feed	902:934	Supplementation of synbiotic at 1 or 1.5 g kg-1 feed to broiler diets during 1-21 d and 0.5 or 0.75 g kg-1 feed, respectively, during 22-42 d improved (P < 0.01) body weight gain, feed conversion ratio (FCR), and decreased (P < 0.01) abdominal fat, serum total cholesterol, and low-density lipoprotein (LDL) cholesterol levels in experiment 1.
30198753	2	74	from	efficacy	229:236	arg1	performance					281:291	growth performance	274:291	growth performance	274:291	Two separate experiments were conducted to evaluate the efficacy of either prebiotic or synbiotic on growth performance, carcass yield, organ weights, and serum metabolic profile of broilers under hot climatic conditions.
30198753	2	74	from	efficacy	229:236	arg1	yield					302:306	carcass yield	294:306	carcass yield	294:306	Two separate experiments were conducted to evaluate the efficacy of either prebiotic or synbiotic on growth performance, carcass yield, organ weights, and serum metabolic profile of broilers under hot climatic conditions.
30198753	2	74	from	efficacy	229:236	arg1	weights					315:321	organ weights	309:321	organ weights	309:321	Two separate experiments were conducted to evaluate the efficacy of either prebiotic or synbiotic on growth performance, carcass yield, organ weights, and serum metabolic profile of broilers under hot climatic conditions.
30198753	2	74	from	efficacy	229:236	arg1	profile					344:350	serum metabolic profile	328:350	serum metabolic profile	328:350	Two separate experiments were conducted to evaluate the efficacy of either prebiotic or synbiotic on growth performance, carcass yield, organ weights, and serum metabolic profile of broilers under hot climatic conditions.
30198753	0	75	theme	carcass	20:26	arg1	criteria					28:35	carcass criteria	20:35	carcass criteria	20:35	Growth performance, carcass criteria, and serum biochemical parameters of broiler chickens supplemented with either synbiotic or prebiotic under hot climatic conditions.
30198753	2	76	theme	separate	177:184	arg1	experiments					186:196	Two separate experiments	173:196	Two separate experiments	173:196	Two separate experiments were conducted to evaluate the efficacy of either prebiotic or synbiotic on growth performance, carcass yield, organ weights, and serum metabolic profile of broilers under hot climatic conditions.
30198753	8	77	theme	total	1138:1142	arg1	cholesterol					1144:1154	serum total cholesterol	1132:1154	serum total cholesterol	1132:1154	Supplementation of synbiotic at 1 or 1.5 g kg-1 feed to broiler diets during 1-21 d and 0.5 or 0.75 g kg-1 feed, respectively, during 22-42 d improved (P < 0.01) body weight gain, feed conversion ratio (FCR), and decreased (P < 0.01) abdominal fat, serum total cholesterol, and low-density lipoprotein (LDL) cholesterol levels in experiment 1.
30198753	10	78	theme	body	1403:1406	arg1	gain					1415:1418	22-42 d improved (P < 0.01) body weight gain	1375:1418	22-42 d improved (P < 0.01) body weight gain	1375:1418	Similarly, supplementation of the prebiotic at 0.5 or 1 g kg-1 in broiler diets during 1-21 d and 0.25 or 0.50 g kg-1 feed, respectively, during 22-42 d improved (P < 0.01) body weight gain, FCR, and decreased (P < 0.01) abdominal fat, serum cholesterol, and LDL-cholesterol levels in experiment 2.
30198753	10	79	theme	P < 0.01	1393:1400	arg1	gain					1415:1418	22-42 d improved (P < 0.01) body weight gain	1375:1418	22-42 d improved (P < 0.01) body weight gain	1375:1418	Similarly, supplementation of the prebiotic at 0.5 or 1 g kg-1 in broiler diets during 1-21 d and 0.25 or 0.50 g kg-1 feed, respectively, during 22-42 d improved (P < 0.01) body weight gain, FCR, and decreased (P < 0.01) abdominal fat, serum cholesterol, and LDL-cholesterol levels in experiment 2.
30198753	0	80	theme	serum	42:46	arg1	parameters					60:69	serum biochemical parameters	42:69	serum biochemical parameters of broiler chickens	42:89	Growth performance, carcass criteria, and serum biochemical parameters of broiler chickens supplemented with either synbiotic or prebiotic under hot climatic conditions.
30198753	8	81	theme	feed	931:934	arg1	Supplementation					883:897	Supplementation	883:897	Supplementation of synbiotic at 1 or 1.5 g kg-1 feed to broiler diets during 1-21 d and 0.5 or 0.75 g kg-1	883:988	Supplementation of synbiotic at 1 or 1.5 g kg-1 feed to broiler diets during 1-21 d and 0.5 or 0.75 g kg-1 feed, respectively, during 22-42 d improved (P < 0.01) body weight gain, feed conversion ratio (FCR), and decreased (P < 0.01) abdominal fat, serum total cholesterol, and low-density lipoprotein (LDL) cholesterol levels in experiment 1.
30198753	8	82	theme	low-density	1161:1171	arg1	levels					1203:1208	low-density lipoprotein (LDL) cholesterol levels	1161:1208	low-density lipoprotein (LDL) cholesterol levels	1161:1208	Supplementation of synbiotic at 1 or 1.5 g kg-1 feed to broiler diets during 1-21 d and 0.5 or 0.75 g kg-1 feed, respectively, during 22-42 d improved (P < 0.01) body weight gain, feed conversion ratio (FCR), and decreased (P < 0.01) abdominal fat, serum total cholesterol, and low-density lipoprotein (LDL) cholesterol levels in experiment 1.
30198753	8	83	from	fat	1127:1129	arg1	experiment					1213:1222	experiment 1	1213:1224	experiment 1	1213:1224	Supplementation of synbiotic at 1 or 1.5 g kg-1 feed to broiler diets during 1-21 d and 0.5 or 0.75 g kg-1 feed, respectively, during 22-42 d improved (P < 0.01) body weight gain, feed conversion ratio (FCR), and decreased (P < 0.01) abdominal fat, serum total cholesterol, and low-density lipoprotein (LDL) cholesterol levels in experiment 1.
30198753	8	84	theme	broiler	939:945	arg1	diets					947:951	broiler diets	939:951	broiler diets	939:951	Supplementation of synbiotic at 1 or 1.5 g kg-1 feed to broiler diets during 1-21 d and 0.5 or 0.75 g kg-1 feed, respectively, during 22-42 d improved (P < 0.01) body weight gain, feed conversion ratio (FCR), and decreased (P < 0.01) abdominal fat, serum total cholesterol, and low-density lipoprotein (LDL) cholesterol levels in experiment 1.
30198753	10	85	from	diets	1304:1308	arg1	supplementation					1241:1255	supplementation	1241:1255	supplementation of the prebiotic at 0.5 or 1 g kg-1 in broiler diets during 1-21 d and 0.25 or 0.50 g kg-1	1241:1346	Similarly, supplementation of the prebiotic at 0.5 or 1 g kg-1 in broiler diets during 1-21 d and 0.25 or 0.50 g kg-1 feed, respectively, during 22-42 d improved (P < 0.01) body weight gain, FCR, and decreased (P < 0.01) abdominal fat, serum cholesterol, and LDL-cholesterol levels in experiment 2.
30198753	10	86	theme	prebiotic	1264:1272	arg1	supplementation					1241:1255	supplementation	1241:1255	supplementation of the prebiotic at 0.5 or 1 g kg-1 in broiler diets during 1-21 d and 0.25 or 0.50 g kg-1	1241:1346	Similarly, supplementation of the prebiotic at 0.5 or 1 g kg-1 in broiler diets during 1-21 d and 0.25 or 0.50 g kg-1 feed, respectively, during 22-42 d improved (P < 0.01) body weight gain, FCR, and decreased (P < 0.01) abdominal fat, serum cholesterol, and LDL-cholesterol levels in experiment 2.
30198753	8	87	theme	P < 0.01	1107:1114	arg1	fat					1127:1129	(P < 0.01) abdominal fat	1106:1129	(P < 0.01) abdominal fat	1106:1129	Supplementation of synbiotic at 1 or 1.5 g kg-1 feed to broiler diets during 1-21 d and 0.5 or 0.75 g kg-1 feed, respectively, during 22-42 d improved (P < 0.01) body weight gain, feed conversion ratio (FCR), and decreased (P < 0.01) abdominal fat, serum total cholesterol, and low-density lipoprotein (LDL) cholesterol levels in experiment 1.
30198753	10	88	theme	improved	1383:1390	arg1	gain					1415:1418	22-42 d improved (P < 0.01) body weight gain	1375:1418	22-42 d improved (P < 0.01) body weight gain	1375:1418	Similarly, supplementation of the prebiotic at 0.5 or 1 g kg-1 in broiler diets during 1-21 d and 0.25 or 0.50 g kg-1 feed, respectively, during 22-42 d improved (P < 0.01) body weight gain, FCR, and decreased (P < 0.01) abdominal fat, serum cholesterol, and LDL-cholesterol levels in experiment 2.
30198753	8	89	from	cholesterol	1144:1154	arg1	experiment					1213:1222	experiment 1	1213:1224	experiment 1	1213:1224	Supplementation of synbiotic at 1 or 1.5 g kg-1 feed to broiler diets during 1-21 d and 0.5 or 0.75 g kg-1 feed, respectively, during 22-42 d improved (P < 0.01) body weight gain, feed conversion ratio (FCR), and decreased (P < 0.01) abdominal fat, serum total cholesterol, and low-density lipoprotein (LDL) cholesterol levels in experiment 1.
29515577	15	0	from	majority	2112:2119	arg1	trials					2163:2168	these anti-DFTD immunization trials	2134:2168	these anti-DFTD immunization trials	2134:2168	The seroconversion in the majority of devils in these anti-DFTD immunization trials was remarkable, especially as DFTD is hallmarked by its immune evasion mechanisms.
29515577	17	1	theme	long-term	2409:2417	arg1	objective					2419:2427	the long-term objective	2405:2427	the long-term objective of releasing devils with immunity to DFTD into the wild	2405:2483	These trials signify the first step in the long-term objective of releasing devils with immunity to DFTD into the wild.
29515577	11	2	theme	adjuvant	1528:1535	arg1	ISCOMATRIX™					1549:1559	the adjuvant combination ISCOMATRIX™	1524:1559	the adjuvant combination ISCOMATRIX™	1524:1559	This compared to the second trial whereby two immunizations comprising DFTD cells and the adjuvant combination ISCOMATRIX™, polyICLC (Hiltonol®) and imiquimod were given a month apart, providing a shorter and, therefore, more practical protocol.
29515577	11	2	theme	adjuvant	1528:1535	arg1	imiquimod					1587:1595	imiquimod	1587:1595	imiquimod	1587:1595	This compared to the second trial whereby two immunizations comprising DFTD cells and the adjuvant combination ISCOMATRIX™, polyICLC (Hiltonol®) and imiquimod were given a month apart, providing a shorter and, therefore, more practical protocol.
29515577	11	2	theme	adjuvant	1528:1535	arg1	polyICLC					1562:1569	polyICLC	1562:1569	polyICLC (Hiltonol®)	1562:1581	This compared to the second trial whereby two immunizations comprising DFTD cells and the adjuvant combination ISCOMATRIX™, polyICLC (Hiltonol®) and imiquimod were given a month apart, providing a shorter and, therefore, more practical protocol.
29515577	10	3	theme	DFTD	1308:1311	arg1	cells					1313:1317	DFTD cells	1308:1317	DFTD cells	1308:1317	The first trial comprised DFTD cells and the adjuvant combination of ISCOMATRIX™, polyIC, and CpG with up to four immunizations given at monthly intervals.
29515577	15	4	from	seroconversion	2090:2103	arg1	majority					2112:2119	the majority	2108:2119	the majority of devils in these anti-DFTD immunization trials	2108:2168	The seroconversion in the majority of devils in these anti-DFTD immunization trials was remarkable, especially as DFTD is hallmarked by its immune evasion mechanisms.
29515577	15	5	from	devils	2124:2129	arg1	trials					2163:2168	these anti-DFTD immunization trials	2134:2168	these anti-DFTD immunization trials	2134:2168	The seroconversion in the majority of devils in these anti-DFTD immunization trials was remarkable, especially as DFTD is hallmarked by its immune evasion mechanisms.
29515577	17	6	theme	first	2391:2395	arg1	step					2397:2400	the first step	2387:2400	the first step in the long-term objective of releasing devils with immunity to DFTD into the wild	2387:2483	These trials signify the first step in the long-term objective of releasing devils with immunity to DFTD into the wild.
29515577	7	7	theme	general	1010:1016	arg1	population					1024:1033	the general devil population	1006:1033	the general devil population	1006:1033	Given the relatively large sample sizes of the trials (N = 19 and N = 33), these responses are likely to reflect those of the general devil population.
29515577	1	8	theme	host	242:245	arg1	system					254:259	the host immune system	238:259	the host immune system	238:259	Devil facial tumor disease (DFTD) is renowned for its successful evasion of the host immune system.
29515577	10	9	theme	adjuvant	1327:1334	arg1	combination					1336:1346	the adjuvant combination	1323:1346	the adjuvant combination of ISCOMATRIX™, polyIC, and CpG with up to four immunizations given at monthly intervals	1323:1435	The first trial comprised DFTD cells and the adjuvant combination of ISCOMATRIX™, polyIC, and CpG with up to four immunizations given at monthly intervals.
29515577	5	10	theme	wild	746:749	arg1	release					751:757	their wild release	740:757	their wild release	740:757	Here, we describe the results of two DFTD immunization trials on cohorts of devils prior to their wild release as part of the Tasmanian Government's Wild Devil Recovery project.
29515577	1	11	theme	facial	168:173	arg1	DFTD					190:193	DFTD	190:193	DFTD	190:193	Devil facial tumor disease (DFTD) is renowned for its successful evasion of the host immune system.
29515577	1	11	theme	facial	168:173	arg1	disease					181:187	Devil facial tumor disease	162:187	Devil facial tumor disease (DFTD)	162:194	Devil facial tumor disease (DFTD) is renowned for its successful evasion of the host immune system.
29515577	8	12	from	basis	1103:1107	arg1	trials					1138:1143	both trials	1133:1143	both trials	1133:1143	DFTD cells manipulated to express MHC-I were used as the antigenic basis of the immunizations in both trials.
29515577	9	13	theme	similar	1233:1239	arg1	levels					1260:1265	similar anti-DFTD antibody levels	1233:1265	similar anti-DFTD antibody levels	1233:1265	Although the adjuvant composition and number of immunizations differed between trials, similar anti-DFTD antibody levels were obtained.
29515577	17	14	from	step	2397:2400	arg1	objective					2419:2427	the long-term objective	2405:2427	the long-term objective of releasing devils with immunity to DFTD into the wild	2405:2483	These trials signify the first step in the long-term objective of releasing devils with immunity to DFTD into the wild.
29515577	4	15	theme	small	628:632	arg1	sizes					641:645	their small sample sizes	622:645	their small sample sizes	622:645	However, these trials were limited by their small sample sizes.
29515577	14	16	theme	adjuvant	1953:1960	arg1	responsible					2073:2083	responsible	2073:2083	responsible	2073:2083	The different adjuvant combination incorporating the RNAase resistant polyICLC and imiquimod used in the second trial is likely to be responsible.
29515577	14	16	theme	adjuvant	1953:1960	arg1	combination					1962:1972	The different adjuvant combination	1939:1972	The different adjuvant combination incorporating the RNAase resistant polyICLC and imiquimod used in the second trial	1939:2055	The different adjuvant combination incorporating the RNAase resistant polyICLC and imiquimod used in the second trial is likely to be responsible.
29515577	14	16	theme	adjuvant	1953:1960	arg1	likely					2060:2065	likely	2060:2065	likely	2060:2065	The different adjuvant combination incorporating the RNAase resistant polyICLC and imiquimod used in the second trial is likely to be responsible.
29515577	13	17	theme	second	1816:1821	arg1	trial					1823:1827	the second trial	1812:1827	the second trial	1812:1827	A key finding was that devils in the second trial responded more quickly and maintained their antibody levels for longer compared to devils in the first trial.
29515577	5	18	theme	Devil	802:806	arg1	project					817:823	the Tasmanian Government's Wild Devil Recovery project	770:823	the Tasmanian Government's Wild Devil Recovery project	770:823	Here, we describe the results of two DFTD immunization trials on cohorts of devils prior to their wild release as part of the Tasmanian Government's Wild Devil Recovery project.
29515577	8	19	theme	antigenic	1093:1101	arg1	cells					1041:1045	DFTD cells	1036:1045	DFTD cells manipulated to express MHC-I	1036:1074	DFTD cells manipulated to express MHC-I were used as the antigenic basis of the immunizations in both trials.
29515577	8	19	theme	antigenic	1093:1101	arg1	basis					1103:1107	the antigenic basis	1089:1107	the antigenic basis of the immunizations in both trials	1089:1143	DFTD cells manipulated to express MHC-I were used as the antigenic basis of the immunizations in both trials.
29515577	14	20	theme	second	2044:2049	arg1	trial					2051:2055	the second trial	2040:2055	the second trial	2040:2055	The different adjuvant combination incorporating the RNAase resistant polyICLC and imiquimod used in the second trial is likely to be responsible.
29515577	11	21	theme	practical	1664:1672	arg1	protocol					1674:1681	a shorter and, therefore, more practical protocol	1633:1681	a shorter and, therefore, more practical protocol	1633:1681	This compared to the second trial whereby two immunizations comprising DFTD cells and the adjuvant combination ISCOMATRIX™, polyICLC (Hiltonol®) and imiquimod were given a month apart, providing a shorter and, therefore, more practical protocol.
29515577	8	22	used	used	1081:1084	arg2	basis					1103:1107	the antigenic basis	1089:1107	the antigenic basis of the immunizations in both trials	1089:1143	DFTD cells manipulated to express MHC-I were used as the antigenic basis of the immunizations in both trials.
29515577	8	22	used	used	1081:1084	arg2	cells					1041:1045	DFTD cells	1036:1045	DFTD cells manipulated to express MHC-I	1036:1074	DFTD cells manipulated to express MHC-I were used as the antigenic basis of the immunizations in both trials.
29515577	0	23	theme	Devils	128:133	arg1	Majority					106:113	the Majority	102:113	the Majority of Tasmanian Devils	102:133	Immunization Strategies Producing a Humoral IgG Immune Response against Devil Facial Tumor Disease in the Majority of Tasmanian Devils Destined for Wild Release.
29515577	5	24	from	results	670:676	arg1	cohorts					713:719	cohorts	713:719	cohorts of devils	713:729	Here, we describe the results of two DFTD immunization trials on cohorts of devils prior to their wild release as part of the Tasmanian Government's Wild Devil Recovery project.
29515577	5	25	theme	Tasmanian	774:782	arg1	project					817:823	the Tasmanian Government's Wild Devil Recovery project	770:823	the Tasmanian Government's Wild Devil Recovery project	770:823	Here, we describe the results of two DFTD immunization trials on cohorts of devils prior to their wild release as part of the Tasmanian Government's Wild Devil Recovery project.
29515577	8	26	theme	immunizations	1116:1128	arg1	cells					1041:1045	DFTD cells	1036:1045	DFTD cells manipulated to express MHC-I	1036:1074	DFTD cells manipulated to express MHC-I were used as the antigenic basis of the immunizations in both trials.
29515577	8	26	theme	immunizations	1116:1128	arg1	basis					1103:1107	the antigenic basis	1089:1107	the antigenic basis of the immunizations in both trials	1089:1143	DFTD cells manipulated to express MHC-I were used as the antigenic basis of the immunizations in both trials.
29515577	6	27	theme	antibody	864:871	arg1	responses					873:881	anti-DFTD antibody responses	854:881	anti-DFTD antibody responses	854:881	95% of the devils developed anti-DFTD antibody responses.
29515577	9	28	theme	anti-DFTD	1241:1249	arg1	levels					1260:1265	similar anti-DFTD antibody levels	1233:1265	similar anti-DFTD antibody levels	1233:1265	Although the adjuvant composition and number of immunizations differed between trials, similar anti-DFTD antibody levels were obtained.
29515577	16	29	theme	immune	2348:2353	arg1	responses					2355:2363	stronger immune responses	2339:2363	stronger immune responses	2339:2363	Microsatellite analyzes of MHC revealed that some MHC-I microsatellites correlated to stronger immune responses.
29515577	15	30	theme	immune	2226:2231	arg1	mechanisms					2241:2250	its immune evasion mechanisms	2222:2250	its immune evasion mechanisms	2222:2250	The seroconversion in the majority of devils in these anti-DFTD immunization trials was remarkable, especially as DFTD is hallmarked by its immune evasion mechanisms.
29515577	3	31	theme	immune-mediated	540:554	arg1	regression					562:571	immune-mediated tumor regression	540:571	immune-mediated tumor regression	540:571	Immunization trials on captive Tasmanian devils have previously demonstrated that an immune response against DFTD can be induced, and that immune-mediated tumor regression can occur.
29515577	11	32	theme	shorter	1635:1641	arg1	protocol					1674:1681	a shorter and, therefore, more practical protocol	1633:1681	a shorter and, therefore, more practical protocol	1633:1681	This compared to the second trial whereby two immunizations comprising DFTD cells and the adjuvant combination ISCOMATRIX™, polyICLC (Hiltonol®) and imiquimod were given a month apart, providing a shorter and, therefore, more practical protocol.
29515577	9	33	theme	adjuvant	1159:1166	arg1	composition					1168:1178	adjuvant composition	1159:1178	adjuvant composition	1159:1178	Although the adjuvant composition and number of immunizations differed between trials, similar anti-DFTD antibody levels were obtained.
29515577	0	34	from	Disease	91:97	arg1	Majority					106:113	the Majority	102:113	the Majority of Tasmanian Devils	102:133	Immunization Strategies Producing a Humoral IgG Immune Response against Devil Facial Tumor Disease in the Majority of Tasmanian Devils Destined for Wild Release.
29515577	5	35	theme	trials	703:708	arg1	results					670:676	the results	666:676	the results of two DFTD immunization trials on cohorts of devils	666:729	Here, we describe the results of two DFTD immunization trials on cohorts of devils prior to their wild release as part of the Tasmanian Government's Wild Devil Recovery project.
29515577	7	36	theme	large	905:909	arg1	sizes					918:922	the relatively large sample sizes	890:922	the relatively large sample sizes of the trials (N = 19 and N = 33)	890:956	Given the relatively large sample sizes of the trials (N = 19 and N = 33), these responses are likely to reflect those of the general devil population.
29515577	0	37	theme	Facial	78:83	arg1	Disease					91:97	Devil Facial Tumor Disease	72:97	Devil Facial Tumor Disease in the Majority of Tasmanian Devils	72:133	Immunization Strategies Producing a Humoral IgG Immune Response against Devil Facial Tumor Disease in the Majority of Tasmanian Devils Destined for Wild Release.
29515577	14	38	theme	resistant	1999:2007	arg1	polyICLC					2009:2016	RNAase resistant polyICLC	1992:2016	RNAase resistant polyICLC	1992:2016	The different adjuvant combination incorporating the RNAase resistant polyICLC and imiquimod used in the second trial is likely to be responsible.
29515577	13	39	theme	key	1781:1783	arg1	finding					1785:1791	A key finding	1779:1791	A key finding	1779:1791	A key finding was that devils in the second trial responded more quickly and maintained their antibody levels for longer compared to devils in the first trial.
29515577	10	40	theme	CpG	1376:1378	arg1	combination					1336:1346	the adjuvant combination	1323:1346	the adjuvant combination of ISCOMATRIX™, polyIC, and CpG with up to four immunizations given at monthly intervals	1323:1435	The first trial comprised DFTD cells and the adjuvant combination of ISCOMATRIX™, polyIC, and CpG with up to four immunizations given at monthly intervals.
29515577	10	40	theme	CpG	1376:1378	arg1	cells					1313:1317	DFTD cells	1308:1317	DFTD cells	1308:1317	The first trial comprised DFTD cells and the adjuvant combination of ISCOMATRIX™, polyIC, and CpG with up to four immunizations given at monthly intervals.
29515577	2	41	theme	primary	365:371	arg1	regulation					267:276	Down regulation	262:276	Down regulation of the major histocompatabilty complex class I molecule (MHC-I) on the DFTD cells	262:358	Down regulation of the major histocompatabilty complex class I molecule (MHC-I) on the DFTD cells is a primary mechanism of immune escape.
29515577	2	41	theme	primary	365:371	arg1	mechanism					373:381	a primary mechanism	363:381	a primary mechanism of immune escape	363:398	Down regulation of the major histocompatabilty complex class I molecule (MHC-I) on the DFTD cells is a primary mechanism of immune escape.
29515577	7	42	dep	trials	931:936	arg1	trials					931:936	the trials	927:936	the trials (N = 19 and N = 33)	927:956	Given the relatively large sample sizes of the trials (N = 19 and N = 33), these responses are likely to reflect those of the general devil population.
29515577	7	42	dep	trials	931:936	arg1	N = 33					950:955	N = 33	950:955	N = 33	950:955	Given the relatively large sample sizes of the trials (N = 19 and N = 33), these responses are likely to reflect those of the general devil population.
29515577	7	42	dep	trials	931:936	arg1	N = 19					939:944	N = 19	939:944	N = 19	939:944	Given the relatively large sample sizes of the trials (N = 19 and N = 33), these responses are likely to reflect those of the general devil population.
29515577	5	43	theme	DFTD	685:688	arg1	trials					703:708	two DFTD immunization trials	681:708	two DFTD immunization trials on cohorts of devils	681:729	Here, we describe the results of two DFTD immunization trials on cohorts of devils prior to their wild release as part of the Tasmanian Government's Wild Devil Recovery project.
29515577	15	44	from	trials	2163:2168	arg1	majority					2112:2119	the majority	2108:2119	the majority of devils in these anti-DFTD immunization trials	2108:2168	The seroconversion in the majority of devils in these anti-DFTD immunization trials was remarkable, especially as DFTD is hallmarked by its immune evasion mechanisms.
29515577	0	45	theme	Devil	72:76	arg1	Disease					91:97	Devil Facial Tumor Disease	72:97	Devil Facial Tumor Disease in the Majority of Tasmanian Devils	72:133	Immunization Strategies Producing a Humoral IgG Immune Response against Devil Facial Tumor Disease in the Majority of Tasmanian Devils Destined for Wild Release.
29515577	0	46	theme	Immunization	0:11	arg1	Strategies					13:22	Immunization Strategies	0:22	Immunization Strategies Producing a Humoral IgG Immune Response against Devil Facial Tumor Disease in the Majority of Tasmanian Devils	0:133	Immunization Strategies Producing a Humoral IgG Immune Response against Devil Facial Tumor Disease in the Majority of Tasmanian Devils Destined for Wild Release.
29515577	1	47	theme	immune	247:252	arg1	system					254:259	the host immune system	238:259	the host immune system	238:259	Devil facial tumor disease (DFTD) is renowned for its successful evasion of the host immune system.
29515577	13	48	from	devils	1802:1807	arg1	trial					1823:1827	the second trial	1812:1827	the second trial	1812:1827	A key finding was that devils in the second trial responded more quickly and maintained their antibody levels for longer compared to devils in the first trial.
29515577	2	49	theme	immune	386:391	arg1	escape					393:398	immune escape	386:398	immune escape	386:398	Down regulation of the major histocompatabilty complex class I molecule (MHC-I) on the DFTD cells is a primary mechanism of immune escape.
29515577	14	50	theme	different	1943:1951	arg1	responsible					2073:2083	responsible	2073:2083	responsible	2073:2083	The different adjuvant combination incorporating the RNAase resistant polyICLC and imiquimod used in the second trial is likely to be responsible.
29515577	14	50	theme	different	1943:1951	arg1	combination					1962:1972	The different adjuvant combination	1939:1972	The different adjuvant combination incorporating the RNAase resistant polyICLC and imiquimod used in the second trial	1939:2055	The different adjuvant combination incorporating the RNAase resistant polyICLC and imiquimod used in the second trial is likely to be responsible.
29515577	14	50	theme	different	1943:1951	arg1	likely					2060:2065	likely	2060:2065	likely	2060:2065	The different adjuvant combination incorporating the RNAase resistant polyICLC and imiquimod used in the second trial is likely to be responsible.
29515577	9	51	theme	antibody	1251:1258	arg1	levels					1260:1265	similar anti-DFTD antibody levels	1233:1265	similar anti-DFTD antibody levels	1233:1265	Although the adjuvant composition and number of immunizations differed between trials, similar anti-DFTD antibody levels were obtained.
29515577	3	52	theme	Immunization	401:412	arg1	trials					414:419	Immunization trials	401:419	Immunization trials on captive Tasmanian devils	401:447	Immunization trials on captive Tasmanian devils have previously demonstrated that an immune response against DFTD can be induced, and that immune-mediated tumor regression can occur.
29515577	0	53	theme	Humoral	36:42	arg1	Response					55:62	a Humoral IgG Immune Response	34:62	a Humoral IgG Immune Response	34:62	Immunization Strategies Producing a Humoral IgG Immune Response against Devil Facial Tumor Disease in the Majority of Tasmanian Devils Destined for Wild Release.
29515577	15	54	theme	devils	2124:2129	arg1	majority					2112:2119	the majority	2108:2119	the majority of devils in these anti-DFTD immunization trials	2108:2168	The seroconversion in the majority of devils in these anti-DFTD immunization trials was remarkable, especially as DFTD is hallmarked by its immune evasion mechanisms.
29515577	5	55	theme	devils	724:729	arg1	cohorts					713:719	cohorts	713:719	cohorts of devils	713:729	Here, we describe the results of two DFTD immunization trials on cohorts of devils prior to their wild release as part of the Tasmanian Government's Wild Devil Recovery project.
29515577	10	56	theme	monthly	1419:1425	arg1	intervals					1427:1435	monthly intervals	1419:1435	monthly intervals	1419:1435	The first trial comprised DFTD cells and the adjuvant combination of ISCOMATRIX™, polyIC, and CpG with up to four immunizations given at monthly intervals.
29515577	15	57	theme	evasion	2233:2239	arg1	mechanisms					2241:2250	its immune evasion mechanisms	2222:2250	its immune evasion mechanisms	2222:2250	The seroconversion in the majority of devils in these anti-DFTD immunization trials was remarkable, especially as DFTD is hallmarked by its immune evasion mechanisms.
29515577	3	58	from	trials	414:419	arg1	devils					442:447	captive Tasmanian devils	424:447	captive Tasmanian devils	424:447	Immunization trials on captive Tasmanian devils have previously demonstrated that an immune response against DFTD can be induced, and that immune-mediated tumor regression can occur.
29515577	2	59	theme	I	323:323	arg1	molecule					325:332	I molecule	323:332	the major histocompatabilty complex class I molecule (MHC-I)	281:340	Down regulation of the major histocompatabilty complex class I molecule (MHC-I) on the DFTD cells is a primary mechanism of immune escape.
29515577	0	60	theme	Immune	48:53	arg1	Response					55:62	a Humoral IgG Immune Response	34:62	a Humoral IgG Immune Response	34:62	Immunization Strategies Producing a Humoral IgG Immune Response against Devil Facial Tumor Disease in the Majority of Tasmanian Devils Destined for Wild Release.
29515577	12	61	theme	booster	1711:1717	arg1	immunization					1719:1730	a booster immunization	1709:1730	a booster immunization	1709:1730	Both trials incorporated a booster immunization given up to 5 months after the primary course.
29515577	11	62	theme	second	1459:1464	arg1	trial					1466:1470	the second trial	1455:1470	the second trial whereby two immunizations comprising DFTD cells and the adjuvant combination ISCOMATRIX™, polyICLC (Hiltonol®) and imiquimod were given a month apart, providing a shorter and, therefore, more practical protocol	1455:1681	This compared to the second trial whereby two immunizations comprising DFTD cells and the adjuvant combination ISCOMATRIX™, polyICLC (Hiltonol®) and imiquimod were given a month apart, providing a shorter and, therefore, more practical protocol.
29515577	2	63	theme	complex	309:315	arg1	class					317:321	the major histocompatabilty complex class I molecule	281:332	the major histocompatabilty complex class I molecule (MHC-I)	281:340	Down regulation of the major histocompatabilty complex class I molecule (MHC-I) on the DFTD cells is a primary mechanism of immune escape.
29515577	2	63	theme	complex	309:315	arg1	MHC-I					335:339	MHC-I	335:339	MHC-I	335:339	Down regulation of the major histocompatabilty complex class I molecule (MHC-I) on the DFTD cells is a primary mechanism of immune escape.
29515577	6	64	theme	devils	837:842	arg1	%					828:828	95%	826:828	95% of the devils	826:842	95% of the devils developed anti-DFTD antibody responses.
29515577	6	64	theme	devils	837:842	arg1	devils					837:842	the devils	833:842	the devils	833:842	95% of the devils developed anti-DFTD antibody responses.
29515577	7	65	theme	devil	1018:1022	arg1	population					1024:1033	the general devil population	1006:1033	the general devil population	1006:1033	Given the relatively large sample sizes of the trials (N = 19 and N = 33), these responses are likely to reflect those of the general devil population.
29515577	9	66	dep	composition	1168:1178	arg1	the					1155:1157	the	1155:1157	the	1155:1157	Although the adjuvant composition and number of immunizations differed between trials, similar anti-DFTD antibody levels were obtained.
29515577	11	67	theme	combination	1537:1547	arg1	ISCOMATRIX™					1549:1559	the adjuvant combination ISCOMATRIX™	1524:1559	the adjuvant combination ISCOMATRIX™	1524:1559	This compared to the second trial whereby two immunizations comprising DFTD cells and the adjuvant combination ISCOMATRIX™, polyICLC (Hiltonol®) and imiquimod were given a month apart, providing a shorter and, therefore, more practical protocol.
29515577	11	67	theme	combination	1537:1547	arg1	imiquimod					1587:1595	imiquimod	1587:1595	imiquimod	1587:1595	This compared to the second trial whereby two immunizations comprising DFTD cells and the adjuvant combination ISCOMATRIX™, polyICLC (Hiltonol®) and imiquimod were given a month apart, providing a shorter and, therefore, more practical protocol.
29515577	11	67	theme	combination	1537:1547	arg1	polyICLC					1562:1569	polyICLC	1562:1569	polyICLC (Hiltonol®)	1562:1581	This compared to the second trial whereby two immunizations comprising DFTD cells and the adjuvant combination ISCOMATRIX™, polyICLC (Hiltonol®) and imiquimod were given a month apart, providing a shorter and, therefore, more practical protocol.
29515577	2	68	theme	Down	262:265	arg1	regulation					267:276	Down regulation	262:276	Down regulation of the major histocompatabilty complex class I molecule (MHC-I) on the DFTD cells	262:358	Down regulation of the major histocompatabilty complex class I molecule (MHC-I) on the DFTD cells is a primary mechanism of immune escape.
29515577	2	68	theme	Down	262:265	arg1	mechanism					373:381	a primary mechanism	363:381	a primary mechanism of immune escape	363:398	Down regulation of the major histocompatabilty complex class I molecule (MHC-I) on the DFTD cells is a primary mechanism of immune escape.
29515577	10	69	with	cells	1313:1317	arg1	immunizations					1396:1408	up to four immunizations	1385:1408	up to four immunizations given at monthly intervals	1385:1435	The first trial comprised DFTD cells and the adjuvant combination of ISCOMATRIX™, polyIC, and CpG with up to four immunizations given at monthly intervals.
29515577	8	70	from	immunizations	1116:1128	arg1	trials					1138:1143	both trials	1133:1143	both trials	1133:1143	DFTD cells manipulated to express MHC-I were used as the antigenic basis of the immunizations in both trials.
29515577	10	71	theme	first	1286:1290	arg1	trial					1292:1296	The first trial	1282:1296	The first trial	1282:1296	The first trial comprised DFTD cells and the adjuvant combination of ISCOMATRIX™, polyIC, and CpG with up to four immunizations given at monthly intervals.
29515577	5	72	theme	Wild	797:800	arg1	project					817:823	the Tasmanian Government's Wild Devil Recovery project	770:823	the Tasmanian Government's Wild Devil Recovery project	770:823	Here, we describe the results of two DFTD immunization trials on cohorts of devils prior to their wild release as part of the Tasmanian Government's Wild Devil Recovery project.
29515577	1	73	theme	Devil	162:166	arg1	DFTD					190:193	DFTD	190:193	DFTD	190:193	Devil facial tumor disease (DFTD) is renowned for its successful evasion of the host immune system.
29515577	1	73	theme	Devil	162:166	arg1	disease					181:187	Devil facial tumor disease	162:187	Devil facial tumor disease (DFTD)	162:194	Devil facial tumor disease (DFTD) is renowned for its successful evasion of the host immune system.
29515577	2	74	theme	major	285:289	arg1	class					317:321	the major histocompatabilty complex class I molecule	281:332	the major histocompatabilty complex class I molecule (MHC-I)	281:340	Down regulation of the major histocompatabilty complex class I molecule (MHC-I) on the DFTD cells is a primary mechanism of immune escape.
29515577	2	74	theme	major	285:289	arg1	MHC-I					335:339	MHC-I	335:339	MHC-I	335:339	Down regulation of the major histocompatabilty complex class I molecule (MHC-I) on the DFTD cells is a primary mechanism of immune escape.
29515577	1	75	theme	tumor	175:179	arg1	DFTD					190:193	DFTD	190:193	DFTD	190:193	Devil facial tumor disease (DFTD) is renowned for its successful evasion of the host immune system.
29515577	1	75	theme	tumor	175:179	arg1	disease					181:187	Devil facial tumor disease	162:187	Devil facial tumor disease (DFTD)	162:194	Devil facial tumor disease (DFTD) is renowned for its successful evasion of the host immune system.
29515577	14	76	dep	polyICLC	2009:2016	arg1	the					1988:1990	the	1988:1990	the	1988:1990	The different adjuvant combination incorporating the RNAase resistant polyICLC and imiquimod used in the second trial is likely to be responsible.
29515577	13	77	from	devils	1912:1917	arg1	trial					1932:1936	the first trial	1922:1936	the first trial	1922:1936	A key finding was that devils in the second trial responded more quickly and maintained their antibody levels for longer compared to devils in the first trial.
29515577	16	78	theme	Microsatellite	2253:2266	arg1	analyzes					2268:2275	Microsatellite analyzes	2253:2275	Microsatellite analyzes of MHC	2253:2282	Microsatellite analyzes of MHC revealed that some MHC-I microsatellites correlated to stronger immune responses.
29515577	3	79	theme	tumor	556:560	arg1	regression					562:571	immune-mediated tumor regression	540:571	immune-mediated tumor regression	540:571	Immunization trials on captive Tasmanian devils have previously demonstrated that an immune response against DFTD can be induced, and that immune-mediated tumor regression can occur.
29515577	0	80	theme	Wild	148:151	arg1	Release					153:159	Wild Release	148:159	Wild Release	148:159	Immunization Strategies Producing a Humoral IgG Immune Response against Devil Facial Tumor Disease in the Majority of Tasmanian Devils Destined for Wild Release.
29515577	8	81	from	trials	1138:1143	arg1	cells					1041:1045	DFTD cells	1036:1045	DFTD cells manipulated to express MHC-I	1036:1074	DFTD cells manipulated to express MHC-I were used as the antigenic basis of the immunizations in both trials.
29515577	8	81	from	trials	1138:1143	arg1	basis					1103:1107	the antigenic basis	1089:1107	the antigenic basis of the immunizations in both trials	1089:1143	DFTD cells manipulated to express MHC-I were used as the antigenic basis of the immunizations in both trials.
29515577	6	82	theme	anti-DFTD	854:862	arg1	responses					873:881	anti-DFTD antibody responses	854:881	anti-DFTD antibody responses	854:881	95% of the devils developed anti-DFTD antibody responses.
29515577	17	83	with	devils	2442:2447	arg1	immunity					2454:2461	immunity	2454:2461	immunity to DFTD	2454:2469	These trials signify the first step in the long-term objective of releasing devils with immunity to DFTD into the wild.
29515577	5	84	from	cohorts	713:719	arg1	results					670:676	the results	666:676	the results of two DFTD immunization trials on cohorts of devils	666:729	Here, we describe the results of two DFTD immunization trials on cohorts of devils prior to their wild release as part of the Tasmanian Government's Wild Devil Recovery project.
29515577	7	85	theme	trials	931:936	arg1	sizes					918:922	the relatively large sample sizes	890:922	the relatively large sample sizes of the trials (N = 19 and N = 33)	890:956	Given the relatively large sample sizes of the trials (N = 19 and N = 33), these responses are likely to reflect those of the general devil population.
29515577	3	86	theme	Tasmanian	432:440	arg1	devils					442:447	captive Tasmanian devils	424:447	captive Tasmanian devils	424:447	Immunization trials on captive Tasmanian devils have previously demonstrated that an immune response against DFTD can be induced, and that immune-mediated tumor regression can occur.
29515577	5	87	theme	immunization	690:701	arg1	trials					703:708	two DFTD immunization trials	681:708	two DFTD immunization trials on cohorts of devils	681:729	Here, we describe the results of two DFTD immunization trials on cohorts of devils prior to their wild release as part of the Tasmanian Government's Wild Devil Recovery project.
29515577	5	88	from	trials	703:708	arg1	cohorts					713:719	cohorts	713:719	cohorts of devils	713:729	Here, we describe the results of two DFTD immunization trials on cohorts of devils prior to their wild release as part of the Tasmanian Government's Wild Devil Recovery project.
29515577	5	89	theme	Recovery	808:815	arg1	project					817:823	the Tasmanian Government's Wild Devil Recovery project	770:823	the Tasmanian Government's Wild Devil Recovery project	770:823	Here, we describe the results of two DFTD immunization trials on cohorts of devils prior to their wild release as part of the Tasmanian Government's Wild Devil Recovery project.
29515577	15	90	theme	immunization	2150:2161	arg1	trials					2163:2168	these anti-DFTD immunization trials	2134:2168	these anti-DFTD immunization trials	2134:2168	The seroconversion in the majority of devils in these anti-DFTD immunization trials was remarkable, especially as DFTD is hallmarked by its immune evasion mechanisms.
29515577	4	91	theme	sample	634:639	arg1	sizes					641:645	their small sample sizes	622:645	their small sample sizes	622:645	However, these trials were limited by their small sample sizes.
29515577	0	92	theme	Tumor	85:89	arg1	Disease					91:97	Devil Facial Tumor Disease	72:97	Devil Facial Tumor Disease in the Majority of Tasmanian Devils	72:133	Immunization Strategies Producing a Humoral IgG Immune Response against Devil Facial Tumor Disease in the Majority of Tasmanian Devils Destined for Wild Release.
29515577	7	93	theme	sample	911:916	arg1	sizes					918:922	the relatively large sample sizes	890:922	the relatively large sample sizes of the trials (N = 19 and N = 33)	890:956	Given the relatively large sample sizes of the trials (N = 19 and N = 33), these responses are likely to reflect those of the general devil population.
29515577	2	94	from	regulation	267:276	arg1	cells					354:358	the DFTD cells	345:358	the DFTD cells	345:358	Down regulation of the major histocompatabilty complex class I molecule (MHC-I) on the DFTD cells is a primary mechanism of immune escape.
29515577	10	95	theme	ISCOMATRIX™	1351:1361	arg1	combination					1336:1346	the adjuvant combination	1323:1346	the adjuvant combination of ISCOMATRIX™, polyIC, and CpG with up to four immunizations given at monthly intervals	1323:1435	The first trial comprised DFTD cells and the adjuvant combination of ISCOMATRIX™, polyIC, and CpG with up to four immunizations given at monthly intervals.
29515577	10	95	theme	ISCOMATRIX™	1351:1361	arg1	cells					1313:1317	DFTD cells	1308:1317	DFTD cells	1308:1317	The first trial comprised DFTD cells and the adjuvant combination of ISCOMATRIX™, polyIC, and CpG with up to four immunizations given at monthly intervals.
29515577	9	96	theme	immunizations	1194:1206	arg1	number					1184:1189	number	1184:1189	number	1184:1189	Although the adjuvant composition and number of immunizations differed between trials, similar anti-DFTD antibody levels were obtained.
29515577	9	96	theme	immunizations	1194:1206	arg1	composition					1168:1178	adjuvant composition	1159:1178	adjuvant composition	1159:1178	Although the adjuvant composition and number of immunizations differed between trials, similar anti-DFTD antibody levels were obtained.
29515577	14	97	theme	RNAase	1992:1997	arg1	polyICLC					2009:2016	RNAase resistant polyICLC	1992:2016	RNAase resistant polyICLC	1992:2016	The different adjuvant combination incorporating the RNAase resistant polyICLC and imiquimod used in the second trial is likely to be responsible.
29515577	10	98	dep	four	1391:1394	arg1	to					1388:1389	to	1388:1389	to	1388:1389	The first trial comprised DFTD cells and the adjuvant combination of ISCOMATRIX™, polyIC, and CpG with up to four immunizations given at monthly intervals.
29515577	10	99	with	combination	1336:1346	arg1	immunizations					1396:1408	up to four immunizations	1385:1408	up to four immunizations given at monthly intervals	1385:1435	The first trial comprised DFTD cells and the adjuvant combination of ISCOMATRIX™, polyIC, and CpG with up to four immunizations given at monthly intervals.
29515577	2	100	theme	DFTD	349:352	arg1	cells					354:358	the DFTD cells	345:358	the DFTD cells	345:358	Down regulation of the major histocompatabilty complex class I molecule (MHC-I) on the DFTD cells is a primary mechanism of immune escape.
29515577	13	101	theme	first	1926:1930	arg1	trial					1932:1936	the first trial	1922:1936	the first trial	1922:1936	A key finding was that devils in the second trial responded more quickly and maintained their antibody levels for longer compared to devils in the first trial.
29515577	2	102	theme	escape	393:398	arg1	regulation					267:276	Down regulation	262:276	Down regulation of the major histocompatabilty complex class I molecule (MHC-I) on the DFTD cells	262:358	Down regulation of the major histocompatabilty complex class I molecule (MHC-I) on the DFTD cells is a primary mechanism of immune escape.
29515577	2	102	theme	escape	393:398	arg1	mechanism					373:381	a primary mechanism	363:381	a primary mechanism of immune escape	363:398	Down regulation of the major histocompatabilty complex class I molecule (MHC-I) on the DFTD cells is a primary mechanism of immune escape.
29515577	10	103	theme	polyIC	1364:1369	arg1	combination					1336:1346	the adjuvant combination	1323:1346	the adjuvant combination of ISCOMATRIX™, polyIC, and CpG with up to four immunizations given at monthly intervals	1323:1435	The first trial comprised DFTD cells and the adjuvant combination of ISCOMATRIX™, polyIC, and CpG with up to four immunizations given at monthly intervals.
29515577	10	103	theme	polyIC	1364:1369	arg1	cells					1313:1317	DFTD cells	1308:1317	DFTD cells	1308:1317	The first trial comprised DFTD cells and the adjuvant combination of ISCOMATRIX™, polyIC, and CpG with up to four immunizations given at monthly intervals.
29515577	1	104	theme	system	254:259	arg1	evasion					227:233	its successful evasion	212:233	its successful evasion of the host immune system	212:259	Devil facial tumor disease (DFTD) is renowned for its successful evasion of the host immune system.
29515577	2	105	dep	class	317:321	arg1	molecule					325:332	I molecule	323:332	the major histocompatabilty complex class I molecule (MHC-I)	281:340	Down regulation of the major histocompatabilty complex class I molecule (MHC-I) on the DFTD cells is a primary mechanism of immune escape.
29515577	3	106	theme	captive	424:430	arg1	devils					442:447	captive Tasmanian devils	424:447	captive Tasmanian devils	424:447	Immunization trials on captive Tasmanian devils have previously demonstrated that an immune response against DFTD can be induced, and that immune-mediated tumor regression can occur.
29515577	0	107	theme	IgG	44:46	arg1	Response					55:62	a Humoral IgG Immune Response	34:62	a Humoral IgG Immune Response	34:62	Immunization Strategies Producing a Humoral IgG Immune Response against Devil Facial Tumor Disease in the Majority of Tasmanian Devils Destined for Wild Release.
29515577	15	108	theme	anti-DFTD	2140:2148	arg1	trials					2163:2168	these anti-DFTD immunization trials	2134:2168	these anti-DFTD immunization trials	2134:2168	The seroconversion in the majority of devils in these anti-DFTD immunization trials was remarkable, especially as DFTD is hallmarked by its immune evasion mechanisms.
29515577	12	109	theme	primary	1763:1769	arg1	course					1771:1776	the primary course	1759:1776	the primary course	1759:1776	Both trials incorporated a booster immunization given up to 5 months after the primary course.
29515577	16	110	theme	MHC	2280:2282	arg1	analyzes					2268:2275	Microsatellite analyzes	2253:2275	Microsatellite analyzes of MHC	2253:2282	Microsatellite analyzes of MHC revealed that some MHC-I microsatellites correlated to stronger immune responses.
29515577	16	111	theme	stronger	2339:2346	arg1	responses					2355:2363	stronger immune responses	2339:2363	stronger immune responses	2339:2363	Microsatellite analyzes of MHC revealed that some MHC-I microsatellites correlated to stronger immune responses.
29515577	2	112	theme	class	317:321	arg1	regulation					267:276	Down regulation	262:276	Down regulation of the major histocompatabilty complex class I molecule (MHC-I) on the DFTD cells	262:358	Down regulation of the major histocompatabilty complex class I molecule (MHC-I) on the DFTD cells is a primary mechanism of immune escape.
29515577	2	112	theme	class	317:321	arg1	mechanism					373:381	a primary mechanism	363:381	a primary mechanism of immune escape	363:398	Down regulation of the major histocompatabilty complex class I molecule (MHC-I) on the DFTD cells is a primary mechanism of immune escape.
29515577	13	113	theme	antibody	1873:1880	arg1	levels					1882:1887	their antibody levels	1867:1887	their antibody levels for longer	1867:1898	A key finding was that devils in the second trial responded more quickly and maintained their antibody levels for longer compared to devils in the first trial.
29515577	16	114	theme	MHC-I	2303:2307	arg1	microsatellites					2309:2323	some MHC-I microsatellites	2298:2323	some MHC-I microsatellites	2298:2323	Microsatellite analyzes of MHC revealed that some MHC-I microsatellites correlated to stronger immune responses.
29515577	2	115	theme	histocompatabilty	291:307	arg1	class					317:321	the major histocompatabilty complex class I molecule	281:332	the major histocompatabilty complex class I molecule (MHC-I)	281:340	Down regulation of the major histocompatabilty complex class I molecule (MHC-I) on the DFTD cells is a primary mechanism of immune escape.
29515577	2	115	theme	histocompatabilty	291:307	arg1	MHC-I					335:339	MHC-I	335:339	MHC-I	335:339	Down regulation of the major histocompatabilty complex class I molecule (MHC-I) on the DFTD cells is a primary mechanism of immune escape.
29515577	8	116	theme	DFTD	1036:1039	arg1	cells					1041:1045	DFTD cells	1036:1045	DFTD cells manipulated to express MHC-I	1036:1074	DFTD cells manipulated to express MHC-I were used as the antigenic basis of the immunizations in both trials.
29515577	8	116	theme	DFTD	1036:1039	arg1	basis					1103:1107	the antigenic basis	1089:1107	the antigenic basis of the immunizations in both trials	1089:1143	DFTD cells manipulated to express MHC-I were used as the antigenic basis of the immunizations in both trials.
29515577	11	117	theme	DFTD	1509:1512	arg1	cells					1514:1518	DFTD cells	1509:1518	DFTD cells	1509:1518	This compared to the second trial whereby two immunizations comprising DFTD cells and the adjuvant combination ISCOMATRIX™, polyICLC (Hiltonol®) and imiquimod were given a month apart, providing a shorter and, therefore, more practical protocol.
29515577	3	118	theme	immune	486:491	arg1	response					493:500	an immune response	483:500	an immune response against DFTD	483:513	Immunization trials on captive Tasmanian devils have previously demonstrated that an immune response against DFTD can be induced, and that immune-mediated tumor regression can occur.
29515577	1	119	theme	successful	216:225	arg1	evasion					227:233	its successful evasion	212:233	its successful evasion of the host immune system	212:259	Devil facial tumor disease (DFTD) is renowned for its successful evasion of the host immune system.
30544575	9	0	from	effects	1391:1397	arg1	mice					1402:1405	mice	1402:1405	mice	1402:1405	Additionally, the extract demonstrated both central and peripheral anti-nociceptive effects in mice, as manifested by a decrease in the count of writhing, induced with acetic acid and an increase in the latency time in the hot plate test.
30544575	8	1	from	injection	1270:1278	arg1	mice					1301:1304	mice	1301:1304	mice	1301:1304	Furthermore, it possessed antipyretic effects against fever induced by subcutaneous injection of Brewer's yeast in mice.
30544575	9	2	theme	latency	1510:1516	arg1	time					1518:1521	the latency time	1506:1521	the latency time in the hot plate test	1506:1543	Additionally, the extract demonstrated both central and peripheral anti-nociceptive effects in mice, as manifested by a decrease in the count of writhing, induced with acetic acid and an increase in the latency time in the hot plate test.
30544575	1	3	theme	several	229:235	arg1	conditions					244:253	several health conditions	229:253	several health conditions	229:253	Reactive oxygen species (ROS) have been linked to several health conditions, among them inflammation.
30544575	7	4	theme	carrageenan-induced	1052:1070	arg1	edema					1085:1089	carrageenan-induced rat hind paw edema	1052:1089	carrageenan-induced rat hind paw edema	1052:1089	The extract exhibited anti-inflammatory effects against carrageenan-induced rat hind paw edema, and suppressed leukocyte infiltration into the peritoneal cavity in carrageenan-treated mice.
30544575	7	5	theme	leukocyte	1107:1115	arg1	infiltration					1117:1128	leukocyte infiltration	1107:1128	leukocyte infiltration into the peritoneal cavity in carrageenan-treated mice	1107:1183	The extract exhibited anti-inflammatory effects against carrageenan-induced rat hind paw edema, and suppressed leukocyte infiltration into the peritoneal cavity in carrageenan-treated mice.
30544575	9	6	theme	writhing	1452:1459	arg1	count					1443:1447	the count	1439:1447	the count	1439:1447	Additionally, the extract demonstrated both central and peripheral anti-nociceptive effects in mice, as manifested by a decrease in the count of writhing, induced with acetic acid and an increase in the latency time in the hot plate test.
30544575	3	7	theme	antipyretic	505:515	arg1	effects					517:523	its possible antioxidant, anti-inflammatory, anti-nociceptive, and antipyretic effects	438:523	its possible antioxidant, anti-inflammatory, anti-nociceptive, and antipyretic effects	438:523	Our study aimed to investigate the chemical composition of a methanol leaf extract from Alpinia zerumbet and its possible antioxidant, anti-inflammatory, anti-nociceptive, and antipyretic effects.
30544575	0	8	from	Activities	159:168	arg1	Polyphenolics					28:40	Polyphenolics	28:40	Polyphenolics of a Leaf Extract from Alpinia zerumbet (Zingiberaceae) and Its Anti-Inflammatory, Anti-Nociceptive, and Antipyretic Activities In Vivo	28:176	HPLC-ESI-MS/MS Profiling of Polyphenolics of a Leaf Extract from Alpinia zerumbet (Zingiberaceae) and Its Anti-Inflammatory, Anti-Nociceptive, and Antipyretic Activities In Vivo.
30544575	0	8	from	Activities	159:168	arg1	Extract					52:58	a Leaf Extract	45:58	a Leaf Extract from Alpinia zerumbet (Zingiberaceae) and Its Anti-Inflammatory, Anti-Nociceptive, and Antipyretic Activities In Vivo	45:176	HPLC-ESI-MS/MS Profiling of Polyphenolics of a Leaf Extract from Alpinia zerumbet (Zingiberaceae) and Its Anti-Inflammatory, Anti-Nociceptive, and Antipyretic Activities In Vivo.
30544575	5	9	dep	in	697:698	arg1	vitro					700:704	vitro	700:704	vitro	700:704	The extract showed substantial in vitro antioxidant effects, and inhibited both cyclooxygenase 1 (COX-1) and cyclooxygenase 2 (COX-2) in vitro, with a higher selectivity towards COX-2.
30544575	1	10	dep	them	262:265	arg1	inflammation					267:278	inflammation	267:278	them inflammation	262:278	Reactive oxygen species (ROS) have been linked to several health conditions, among them inflammation.
30544575	9	11	from	time	1518:1521	arg1	test					1540:1543	the hot plate test	1526:1543	the hot plate test	1526:1543	Additionally, the extract demonstrated both central and peripheral anti-nociceptive effects in mice, as manifested by a decrease in the count of writhing, induced with acetic acid and an increase in the latency time in the hot plate test.
30544575	6	12	theme	LOX	885:887	arg1	activity					890:897	5-lipoxygenase (LOX) activity	869:897	5-lipoxygenase (LOX) activity	869:897	It also inhibited 5-lipoxygenase (LOX) activity in vitro with nearly double the potency of zileuton, a reference 5-lipoxygenase (LOX) inhibitor.
30544575	3	13	from	effects	517:523	arg1	composition					373:383	the chemical composition	360:383	the chemical composition of a methanol leaf extract from Alpinia zerumbet and its possible antioxidant, anti-inflammatory, anti-nociceptive, and antipyretic effects	360:523	Our study aimed to investigate the chemical composition of a methanol leaf extract from Alpinia zerumbet and its possible antioxidant, anti-inflammatory, anti-nociceptive, and antipyretic effects.
30544575	3	13	from	effects	517:523	arg1	extract					404:410	a methanol leaf extract	388:410	a methanol leaf extract from Alpinia zerumbet and its possible antioxidant, anti-inflammatory, anti-nociceptive, and antipyretic effects	388:523	Our study aimed to investigate the chemical composition of a methanol leaf extract from Alpinia zerumbet and its possible antioxidant, anti-inflammatory, anti-nociceptive, and antipyretic effects.
30544575	10	14	dep	inflammation	1673:1684	arg1	disorders					1702:1710	related disorders	1694:1710	related disorders	1694:1710	These findings suggest that the leaf extract from Alpinia zerumbet could be a candidate for the development of a drug to treat inflammation and ROS related disorders.
30544575	6	15	theme	5-lipoxygenase	869:882	arg1	activity					890:897	5-lipoxygenase (LOX) activity	869:897	5-lipoxygenase (LOX) activity	869:897	It also inhibited 5-lipoxygenase (LOX) activity in vitro with nearly double the potency of zileuton, a reference 5-lipoxygenase (LOX) inhibitor.
30544575	7	16	theme	paw	1081:1083	arg1	edema					1085:1089	carrageenan-induced rat hind paw edema	1052:1089	carrageenan-induced rat hind paw edema	1052:1089	The extract exhibited anti-inflammatory effects against carrageenan-induced rat hind paw edema, and suppressed leukocyte infiltration into the peritoneal cavity in carrageenan-treated mice.
30544575	9	17	theme	hot	1530:1532	arg1	test					1540:1543	the hot plate test	1526:1543	the hot plate test	1526:1543	Additionally, the extract demonstrated both central and peripheral anti-nociceptive effects in mice, as manifested by a decrease in the count of writhing, induced with acetic acid and an increase in the latency time in the hot plate test.
30544575	8	18	theme	subcutaneous	1257:1268	arg1	injection					1270:1278	subcutaneous injection	1257:1278	subcutaneous injection of Brewer's yeast in mice	1257:1304	Furthermore, it possessed antipyretic effects against fever induced by subcutaneous injection of Brewer's yeast in mice.
30544575	0	19	theme	Leaf	47:50	arg1	Extract					52:58	a Leaf Extract	45:58	a Leaf Extract from Alpinia zerumbet (Zingiberaceae) and Its Anti-Inflammatory, Anti-Nociceptive, and Antipyretic Activities In Vivo	45:176	HPLC-ESI-MS/MS Profiling of Polyphenolics of a Leaf Extract from Alpinia zerumbet (Zingiberaceae) and Its Anti-Inflammatory, Anti-Nociceptive, and Antipyretic Activities In Vivo.
30544575	3	20	from	composition	373:383	arg1	zerumbet					425:432	Alpinia zerumbet	417:432	Alpinia zerumbet	417:432	Our study aimed to investigate the chemical composition of a methanol leaf extract from Alpinia zerumbet and its possible antioxidant, anti-inflammatory, anti-nociceptive, and antipyretic effects.
30544575	3	20	from	composition	373:383	arg1	effects					517:523	its possible antioxidant, anti-inflammatory, anti-nociceptive, and antipyretic effects	438:523	its possible antioxidant, anti-inflammatory, anti-nociceptive, and antipyretic effects	438:523	Our study aimed to investigate the chemical composition of a methanol leaf extract from Alpinia zerumbet and its possible antioxidant, anti-inflammatory, anti-nociceptive, and antipyretic effects.
30544575	4	21	theme	acid	586:589	arg1	derivatives					591:601	benzoic and cinnamic acid derivatives	565:601	benzoic and cinnamic acid derivatives	565:601	Altogether, 37 compounds, representing benzoic and cinnamic acid derivatives and flavonoids (aglycones and glycosides), were characterized.
30544575	9	22	theme	plate	1534:1538	arg1	test					1540:1543	the hot plate test	1526:1543	the hot plate test	1526:1543	Additionally, the extract demonstrated both central and peripheral anti-nociceptive effects in mice, as manifested by a decrease in the count of writhing, induced with acetic acid and an increase in the latency time in the hot plate test.
30544575	7	23	theme	carrageenan-treated	1160:1178	arg1	mice					1180:1183	carrageenan-treated mice	1160:1183	carrageenan-treated mice	1160:1183	The extract exhibited anti-inflammatory effects against carrageenan-induced rat hind paw edema, and suppressed leukocyte infiltration into the peritoneal cavity in carrageenan-treated mice.
30544575	7	24	theme	rat	1072:1074	arg1	edema					1085:1089	carrageenan-induced rat hind paw edema	1052:1089	carrageenan-induced rat hind paw edema	1052:1089	The extract exhibited anti-inflammatory effects against carrageenan-induced rat hind paw edema, and suppressed leukocyte infiltration into the peritoneal cavity in carrageenan-treated mice.
30544575	9	25	from	increase	1494:1501	arg1	count					1443:1447	the count	1439:1447	the count	1439:1447	Additionally, the extract demonstrated both central and peripheral anti-nociceptive effects in mice, as manifested by a decrease in the count of writhing, induced with acetic acid and an increase in the latency time in the hot plate test.
30544575	9	25	from	increase	1494:1501	arg1	time					1518:1521	the latency time	1506:1521	the latency time in the hot plate test	1506:1543	Additionally, the extract demonstrated both central and peripheral anti-nociceptive effects in mice, as manifested by a decrease in the count of writhing, induced with acetic acid and an increase in the latency time in the hot plate test.
30544575	1	26	theme	Reactive	179:186	arg1	ROS					204:206	ROS	204:206	ROS	204:206	Reactive oxygen species (ROS) have been linked to several health conditions, among them inflammation.
30544575	1	26	theme	Reactive	179:186	arg1	species					195:201	Reactive oxygen species	179:201	Reactive oxygen species (ROS)	179:207	Reactive oxygen species (ROS) have been linked to several health conditions, among them inflammation.
30544575	0	27	theme	HPLC-ESI-MS/MS	0:13	arg1	Profiling					15:23	HPLC-ESI-MS/MS Profiling	0:23	HPLC-ESI-MS/MS Profiling of Polyphenolics of a Leaf Extract from Alpinia zerumbet (Zingiberaceae) and Its Anti-Inflammatory, Anti-Nociceptive, and Antipyretic Activities In Vivo.	0:177	HPLC-ESI-MS/MS Profiling of Polyphenolics of a Leaf Extract from Alpinia zerumbet (Zingiberaceae) and Its Anti-Inflammatory, Anti-Nociceptive, and Antipyretic Activities In Vivo.
30544575	6	28	theme	zileuton	942:949	arg1	potency					931:937	nearly double the potency	913:937	nearly double the potency of zileuton, a reference 5-lipoxygenase (LOX) inhibitor	913:993	It also inhibited 5-lipoxygenase (LOX) activity in vitro with nearly double the potency of zileuton, a reference 5-lipoxygenase (LOX) inhibitor.
30544575	7	29	theme	hind	1076:1079	arg1	edema					1085:1089	carrageenan-induced rat hind paw edema	1052:1089	carrageenan-induced rat hind paw edema	1052:1089	The extract exhibited anti-inflammatory effects against carrageenan-induced rat hind paw edema, and suppressed leukocyte infiltration into the peritoneal cavity in carrageenan-treated mice.
30544575	0	30	dep	zerumbet	73:80	arg1	Zingiberaceae					83:95	Zingiberaceae	83:95	Zingiberaceae	83:95	HPLC-ESI-MS/MS Profiling of Polyphenolics of a Leaf Extract from Alpinia zerumbet (Zingiberaceae) and Its Anti-Inflammatory, Anti-Nociceptive, and Antipyretic Activities In Vivo.
30544575	6	31	theme	5-lipoxygenase	964:977	arg1	zileuton					942:949	zileuton	942:949	zileuton	942:949	It also inhibited 5-lipoxygenase (LOX) activity in vitro with nearly double the potency of zileuton, a reference 5-lipoxygenase (LOX) inhibitor.
30544575	6	31	theme	5-lipoxygenase	964:977	arg1	inhibitor					985:993	a reference 5-lipoxygenase (LOX) inhibitor	952:993	a reference 5-lipoxygenase (LOX) inhibitor	952:993	It also inhibited 5-lipoxygenase (LOX) activity in vitro with nearly double the potency of zileuton, a reference 5-lipoxygenase (LOX) inhibitor.
30544575	3	32	theme	Alpinia	417:423	arg1	zerumbet					425:432	Alpinia zerumbet	417:432	Alpinia zerumbet	417:432	Our study aimed to investigate the chemical composition of a methanol leaf extract from Alpinia zerumbet and its possible antioxidant, anti-inflammatory, anti-nociceptive, and antipyretic effects.
30544575	0	33	theme	Polyphenolics	28:40	arg1	Profiling					15:23	HPLC-ESI-MS/MS Profiling	0:23	HPLC-ESI-MS/MS Profiling of Polyphenolics of a Leaf Extract from Alpinia zerumbet (Zingiberaceae) and Its Anti-Inflammatory, Anti-Nociceptive, and Antipyretic Activities In Vivo.	0:177	HPLC-ESI-MS/MS Profiling of Polyphenolics of a Leaf Extract from Alpinia zerumbet (Zingiberaceae) and Its Anti-Inflammatory, Anti-Nociceptive, and Antipyretic Activities In Vivo.
30544575	9	34	theme	acetic	1475:1480	arg1	acid					1482:1485	acetic acid	1475:1485	acetic acid	1475:1485	Additionally, the extract demonstrated both central and peripheral anti-nociceptive effects in mice, as manifested by a decrease in the count of writhing, induced with acetic acid and an increase in the latency time in the hot plate test.
30544575	5	35	theme	antioxidant	706:716	arg1	effects					718:724	substantial in vitro antioxidant effects	685:724	substantial in vitro antioxidant effects	685:724	The extract showed substantial in vitro antioxidant effects, and inhibited both cyclooxygenase 1 (COX-1) and cyclooxygenase 2 (COX-2) in vitro, with a higher selectivity towards COX-2.
30544575	1	36	theme	health	237:242	arg1	conditions					244:253	several health conditions	229:253	several health conditions	229:253	Reactive oxygen species (ROS) have been linked to several health conditions, among them inflammation.
30544575	3	37	theme	antioxidant	451:461	arg1	effects					517:523	its possible antioxidant, anti-inflammatory, anti-nociceptive, and antipyretic effects	438:523	its possible antioxidant, anti-inflammatory, anti-nociceptive, and antipyretic effects	438:523	Our study aimed to investigate the chemical composition of a methanol leaf extract from Alpinia zerumbet and its possible antioxidant, anti-inflammatory, anti-nociceptive, and antipyretic effects.
30544575	3	38	theme	chemical	364:371	arg1	composition					373:383	the chemical composition	360:383	the chemical composition of a methanol leaf extract from Alpinia zerumbet and its possible antioxidant, anti-inflammatory, anti-nociceptive, and antipyretic effects	360:523	Our study aimed to investigate the chemical composition of a methanol leaf extract from Alpinia zerumbet and its possible antioxidant, anti-inflammatory, anti-nociceptive, and antipyretic effects.
30544575	9	39	theme	central	1351:1357	arg1	effects					1391:1397	both central and peripheral anti-nociceptive effects	1346:1397	both central and peripheral anti-nociceptive effects in mice	1346:1405	Additionally, the extract demonstrated both central and peripheral anti-nociceptive effects in mice, as manifested by a decrease in the count of writhing, induced with acetic acid and an increase in the latency time in the hot plate test.
30544575	0	40	theme	Anti-Inflammatory	106:122	arg1	Activities					159:168	Its Anti-Inflammatory, Anti-Nociceptive, and Antipyretic Activities	102:168	Its Anti-Inflammatory, Anti-Nociceptive, and Antipyretic Activities In Vivo	102:176	HPLC-ESI-MS/MS Profiling of Polyphenolics of a Leaf Extract from Alpinia zerumbet (Zingiberaceae) and Its Anti-Inflammatory, Anti-Nociceptive, and Antipyretic Activities In Vivo.
30544575	2	41	theme	Natural	281:287	arg1	antioxidants					289:300	Natural antioxidants	281:300	Natural antioxidants	281:300	Natural antioxidants may attenuate this damage.
30544575	4	42	theme	cinnamic	577:584	arg1	derivatives					591:601	benzoic and cinnamic acid derivatives	565:601	benzoic and cinnamic acid derivatives	565:601	Altogether, 37 compounds, representing benzoic and cinnamic acid derivatives and flavonoids (aglycones and glycosides), were characterized.
30544575	3	43	theme	leaf	399:402	arg1	extract					404:410	a methanol leaf extract	388:410	a methanol leaf extract from Alpinia zerumbet and its possible antioxidant, anti-inflammatory, anti-nociceptive, and antipyretic effects	388:523	Our study aimed to investigate the chemical composition of a methanol leaf extract from Alpinia zerumbet and its possible antioxidant, anti-inflammatory, anti-nociceptive, and antipyretic effects.
30544575	4	44	theme	benzoic	565:571	arg1	derivatives					591:601	benzoic and cinnamic acid derivatives	565:601	benzoic and cinnamic acid derivatives	565:601	Altogether, 37 compounds, representing benzoic and cinnamic acid derivatives and flavonoids (aglycones and glycosides), were characterized.
30544575	7	45	theme	anti-inflammatory	1018:1034	arg1	effects					1036:1042	anti-inflammatory effects	1018:1042	anti-inflammatory effects against carrageenan-induced rat hind paw edema	1018:1089	The extract exhibited anti-inflammatory effects against carrageenan-induced rat hind paw edema, and suppressed leukocyte infiltration into the peritoneal cavity in carrageenan-treated mice.
30544575	0	46	from	zerumbet	73:80	arg1	Polyphenolics					28:40	Polyphenolics	28:40	Polyphenolics of a Leaf Extract from Alpinia zerumbet (Zingiberaceae) and Its Anti-Inflammatory, Anti-Nociceptive, and Antipyretic Activities In Vivo	28:176	HPLC-ESI-MS/MS Profiling of Polyphenolics of a Leaf Extract from Alpinia zerumbet (Zingiberaceae) and Its Anti-Inflammatory, Anti-Nociceptive, and Antipyretic Activities In Vivo.
30544575	0	46	from	zerumbet	73:80	arg1	Extract					52:58	a Leaf Extract	45:58	a Leaf Extract from Alpinia zerumbet (Zingiberaceae) and Its Anti-Inflammatory, Anti-Nociceptive, and Antipyretic Activities In Vivo	45:176	HPLC-ESI-MS/MS Profiling of Polyphenolics of a Leaf Extract from Alpinia zerumbet (Zingiberaceae) and Its Anti-Inflammatory, Anti-Nociceptive, and Antipyretic Activities In Vivo.
30544575	1	47	theme	oxygen	188:193	arg1	ROS					204:206	ROS	204:206	ROS	204:206	Reactive oxygen species (ROS) have been linked to several health conditions, among them inflammation.
30544575	1	47	theme	oxygen	188:193	arg1	species					195:201	Reactive oxygen species	179:201	Reactive oxygen species (ROS)	179:207	Reactive oxygen species (ROS) have been linked to several health conditions, among them inflammation.
30544575	5	48	theme	higher	817:822	arg1	selectivity					824:834	a higher selectivity	815:834	a higher selectivity towards COX-2	815:848	The extract showed substantial in vitro antioxidant effects, and inhibited both cyclooxygenase 1 (COX-1) and cyclooxygenase 2 (COX-2) in vitro, with a higher selectivity towards COX-2.
30544575	3	49	theme	extract	404:410	arg1	composition					373:383	the chemical composition	360:383	the chemical composition of a methanol leaf extract from Alpinia zerumbet and its possible antioxidant, anti-inflammatory, anti-nociceptive, and antipyretic effects	360:523	Our study aimed to investigate the chemical composition of a methanol leaf extract from Alpinia zerumbet and its possible antioxidant, anti-inflammatory, anti-nociceptive, and antipyretic effects.
30544575	6	50	theme	double	920:925	arg1	potency					931:937	nearly double the potency	913:937	nearly double the potency of zileuton, a reference 5-lipoxygenase (LOX) inhibitor	913:993	It also inhibited 5-lipoxygenase (LOX) activity in vitro with nearly double the potency of zileuton, a reference 5-lipoxygenase (LOX) inhibitor.
30544575	0	51	theme	Anti-Nociceptive	125:140	arg1	Activities					159:168	Its Anti-Inflammatory, Anti-Nociceptive, and Antipyretic Activities	102:168	Its Anti-Inflammatory, Anti-Nociceptive, and Antipyretic Activities In Vivo	102:176	HPLC-ESI-MS/MS Profiling of Polyphenolics of a Leaf Extract from Alpinia zerumbet (Zingiberaceae) and Its Anti-Inflammatory, Anti-Nociceptive, and Antipyretic Activities In Vivo.
30544575	6	52	theme	reference	954:962	arg1	zileuton					942:949	zileuton	942:949	zileuton	942:949	It also inhibited 5-lipoxygenase (LOX) activity in vitro with nearly double the potency of zileuton, a reference 5-lipoxygenase (LOX) inhibitor.
30544575	6	52	theme	reference	954:962	arg1	inhibitor					985:993	a reference 5-lipoxygenase (LOX) inhibitor	952:993	a reference 5-lipoxygenase (LOX) inhibitor	952:993	It also inhibited 5-lipoxygenase (LOX) activity in vitro with nearly double the potency of zileuton, a reference 5-lipoxygenase (LOX) inhibitor.
30544575	7	53	from	infiltration	1117:1128	arg1	mice					1180:1183	carrageenan-treated mice	1160:1183	carrageenan-treated mice	1160:1183	The extract exhibited anti-inflammatory effects against carrageenan-induced rat hind paw edema, and suppressed leukocyte infiltration into the peritoneal cavity in carrageenan-treated mice.
30544575	3	54	theme	possible	442:449	arg1	effects					517:523	its possible antioxidant, anti-inflammatory, anti-nociceptive, and antipyretic effects	438:523	its possible antioxidant, anti-inflammatory, anti-nociceptive, and antipyretic effects	438:523	Our study aimed to investigate the chemical composition of a methanol leaf extract from Alpinia zerumbet and its possible antioxidant, anti-inflammatory, anti-nociceptive, and antipyretic effects.
30544575	0	55	theme	Extract	52:58	arg1	Polyphenolics					28:40	Polyphenolics	28:40	Polyphenolics of a Leaf Extract from Alpinia zerumbet (Zingiberaceae) and Its Anti-Inflammatory, Anti-Nociceptive, and Antipyretic Activities In Vivo	28:176	HPLC-ESI-MS/MS Profiling of Polyphenolics of a Leaf Extract from Alpinia zerumbet (Zingiberaceae) and Its Anti-Inflammatory, Anti-Nociceptive, and Antipyretic Activities In Vivo.
30544575	9	56	theme	peripheral	1363:1372	arg1	effects					1391:1397	both central and peripheral anti-nociceptive effects	1346:1397	both central and peripheral anti-nociceptive effects in mice	1346:1405	Additionally, the extract demonstrated both central and peripheral anti-nociceptive effects in mice, as manifested by a decrease in the count of writhing, induced with acetic acid and an increase in the latency time in the hot plate test.
30544575	0	57	theme	Antipyretic	147:157	arg1	Activities					159:168	Its Anti-Inflammatory, Anti-Nociceptive, and Antipyretic Activities	102:168	Its Anti-Inflammatory, Anti-Nociceptive, and Antipyretic Activities In Vivo	102:176	HPLC-ESI-MS/MS Profiling of Polyphenolics of a Leaf Extract from Alpinia zerumbet (Zingiberaceae) and Its Anti-Inflammatory, Anti-Nociceptive, and Antipyretic Activities In Vivo.
30544575	8	58	contain	possessed	1202:1210	arg2	effects					1224:1230	antipyretic effects	1212:1230	antipyretic effects	1212:1230	Furthermore, it possessed antipyretic effects against fever induced by subcutaneous injection of Brewer's yeast in mice.
30544575	8	58	contain	possessed	1202:1210	arg1	it					1199:1200	it	1199:1200	it	1199:1200	Furthermore, it possessed antipyretic effects against fever induced by subcutaneous injection of Brewer's yeast in mice.
30544575	10	59	from	zerumbet	1604:1611	arg1	candidate					1624:1632	a candidate	1622:1632	a candidate for the development of a drug to treat inflammation and ROS related disorders	1622:1710	These findings suggest that the leaf extract from Alpinia zerumbet could be a candidate for the development of a drug to treat inflammation and ROS related disorders.
30544575	10	59	from	zerumbet	1604:1611	arg1	extract					1583:1589	the leaf extract	1574:1589	the leaf extract from Alpinia zerumbet	1574:1611	These findings suggest that the leaf extract from Alpinia zerumbet could be a candidate for the development of a drug to treat inflammation and ROS related disorders.
30544575	3	60	theme	anti-nociceptive	483:498	arg1	effects					517:523	its possible antioxidant, anti-inflammatory, anti-nociceptive, and antipyretic effects	438:523	its possible antioxidant, anti-inflammatory, anti-nociceptive, and antipyretic effects	438:523	Our study aimed to investigate the chemical composition of a methanol leaf extract from Alpinia zerumbet and its possible antioxidant, anti-inflammatory, anti-nociceptive, and antipyretic effects.
30544575	3	61	theme	methanol	390:397	arg1	extract					404:410	a methanol leaf extract	388:410	a methanol leaf extract from Alpinia zerumbet and its possible antioxidant, anti-inflammatory, anti-nociceptive, and antipyretic effects	388:523	Our study aimed to investigate the chemical composition of a methanol leaf extract from Alpinia zerumbet and its possible antioxidant, anti-inflammatory, anti-nociceptive, and antipyretic effects.
30544575	5	62	theme	substantial	685:695	arg1	effects					718:724	substantial in vitro antioxidant effects	685:724	substantial in vitro antioxidant effects	685:724	The extract showed substantial in vitro antioxidant effects, and inhibited both cyclooxygenase 1 (COX-1) and cyclooxygenase 2 (COX-2) in vitro, with a higher selectivity towards COX-2.
30544575	1	63	attach	linked	219:224	arg2	ROS					204:206	ROS	204:206	ROS	204:206	Reactive oxygen species (ROS) have been linked to several health conditions, among them inflammation.
30544575	1	63	attach	linked	219:224	arg1	conditions					244:253	several health conditions	229:253	several health conditions	229:253	Reactive oxygen species (ROS) have been linked to several health conditions, among them inflammation.
30544575	1	63	attach	linked	219:224	arg2	species					195:201	Reactive oxygen species	179:201	Reactive oxygen species (ROS)	179:207	Reactive oxygen species (ROS) have been linked to several health conditions, among them inflammation.
30544575	8	64	theme	antipyretic	1212:1222	arg1	effects					1224:1230	antipyretic effects	1212:1230	antipyretic effects	1212:1230	Furthermore, it possessed antipyretic effects against fever induced by subcutaneous injection of Brewer's yeast in mice.
30544575	5	65	theme	in	697:698	arg1	effects					718:724	substantial in vitro antioxidant effects	685:724	substantial in vitro antioxidant effects	685:724	The extract showed substantial in vitro antioxidant effects, and inhibited both cyclooxygenase 1 (COX-1) and cyclooxygenase 2 (COX-2) in vitro, with a higher selectivity towards COX-2.
30544575	9	66	from	decrease	1427:1434	arg1	count					1443:1447	the count	1439:1447	the count	1439:1447	Additionally, the extract demonstrated both central and peripheral anti-nociceptive effects in mice, as manifested by a decrease in the count of writhing, induced with acetic acid and an increase in the latency time in the hot plate test.
30544575	9	66	from	decrease	1427:1434	arg1	time					1518:1521	the latency time	1506:1521	the latency time in the hot plate test	1506:1543	Additionally, the extract demonstrated both central and peripheral anti-nociceptive effects in mice, as manifested by a decrease in the count of writhing, induced with acetic acid and an increase in the latency time in the hot plate test.
30544575	3	67	theme	anti-inflammatory	464:480	arg1	effects					517:523	its possible antioxidant, anti-inflammatory, anti-nociceptive, and antipyretic effects	438:523	its possible antioxidant, anti-inflammatory, anti-nociceptive, and antipyretic effects	438:523	Our study aimed to investigate the chemical composition of a methanol leaf extract from Alpinia zerumbet and its possible antioxidant, anti-inflammatory, anti-nociceptive, and antipyretic effects.
30544575	10	68	theme	drug	1659:1662	arg1	development					1642:1652	the development	1638:1652	the development of a drug to treat inflammation and ROS related disorders	1638:1710	These findings suggest that the leaf extract from Alpinia zerumbet could be a candidate for the development of a drug to treat inflammation and ROS related disorders.
30544575	0	69	theme	Alpinia	65:71	arg1	zerumbet					73:80	Alpinia zerumbet	65:80	Alpinia zerumbet (Zingiberaceae)	65:96	HPLC-ESI-MS/MS Profiling of Polyphenolics of a Leaf Extract from Alpinia zerumbet (Zingiberaceae) and Its Anti-Inflammatory, Anti-Nociceptive, and Antipyretic Activities In Vivo.
30544575	4	70	dep	flavonoids	607:616	arg1	glycosides					633:642	glycosides	633:642	glycosides	633:642	Altogether, 37 compounds, representing benzoic and cinnamic acid derivatives and flavonoids (aglycones and glycosides), were characterized.
30544575	4	70	dep	flavonoids	607:616	arg1	aglycones					619:627	aglycones	619:627	aglycones	619:627	Altogether, 37 compounds, representing benzoic and cinnamic acid derivatives and flavonoids (aglycones and glycosides), were characterized.
30544575	10	71	theme	related	1694:1700	arg1	disorders					1702:1710	related disorders	1694:1710	related disorders	1694:1710	These findings suggest that the leaf extract from Alpinia zerumbet could be a candidate for the development of a drug to treat inflammation and ROS related disorders.
30544575	7	72	theme	peritoneal	1139:1148	arg1	cavity					1150:1155	the peritoneal cavity	1135:1155	the peritoneal cavity	1135:1155	The extract exhibited anti-inflammatory effects against carrageenan-induced rat hind paw edema, and suppressed leukocyte infiltration into the peritoneal cavity in carrageenan-treated mice.
30544575	6	73	theme	LOX	980:982	arg1	zileuton					942:949	zileuton	942:949	zileuton	942:949	It also inhibited 5-lipoxygenase (LOX) activity in vitro with nearly double the potency of zileuton, a reference 5-lipoxygenase (LOX) inhibitor.
30544575	6	73	theme	LOX	980:982	arg1	inhibitor					985:993	a reference 5-lipoxygenase (LOX) inhibitor	952:993	a reference 5-lipoxygenase (LOX) inhibitor	952:993	It also inhibited 5-lipoxygenase (LOX) activity in vitro with nearly double the potency of zileuton, a reference 5-lipoxygenase (LOX) inhibitor.
30544575	0	74	from	Polyphenolics	28:40	arg1	zerumbet					73:80	Alpinia zerumbet	65:80	Alpinia zerumbet (Zingiberaceae)	65:96	HPLC-ESI-MS/MS Profiling of Polyphenolics of a Leaf Extract from Alpinia zerumbet (Zingiberaceae) and Its Anti-Inflammatory, Anti-Nociceptive, and Antipyretic Activities In Vivo.
30544575	0	74	from	Polyphenolics	28:40	arg1	Activities					159:168	Its Anti-Inflammatory, Anti-Nociceptive, and Antipyretic Activities	102:168	Its Anti-Inflammatory, Anti-Nociceptive, and Antipyretic Activities In Vivo	102:176	HPLC-ESI-MS/MS Profiling of Polyphenolics of a Leaf Extract from Alpinia zerumbet (Zingiberaceae) and Its Anti-Inflammatory, Anti-Nociceptive, and Antipyretic Activities In Vivo.
30544575	3	75	from	zerumbet	425:432	arg1	composition					373:383	the chemical composition	360:383	the chemical composition of a methanol leaf extract from Alpinia zerumbet and its possible antioxidant, anti-inflammatory, anti-nociceptive, and antipyretic effects	360:523	Our study aimed to investigate the chemical composition of a methanol leaf extract from Alpinia zerumbet and its possible antioxidant, anti-inflammatory, anti-nociceptive, and antipyretic effects.
30544575	3	75	from	zerumbet	425:432	arg1	extract					404:410	a methanol leaf extract	388:410	a methanol leaf extract from Alpinia zerumbet and its possible antioxidant, anti-inflammatory, anti-nociceptive, and antipyretic effects	388:523	Our study aimed to investigate the chemical composition of a methanol leaf extract from Alpinia zerumbet and its possible antioxidant, anti-inflammatory, anti-nociceptive, and antipyretic effects.
30544575	10	76	theme	leaf	1578:1581	arg1	candidate					1624:1632	a candidate	1622:1632	a candidate for the development of a drug to treat inflammation and ROS related disorders	1622:1710	These findings suggest that the leaf extract from Alpinia zerumbet could be a candidate for the development of a drug to treat inflammation and ROS related disorders.
30544575	10	76	theme	leaf	1578:1581	arg1	extract					1583:1589	the leaf extract	1574:1589	the leaf extract from Alpinia zerumbet	1574:1611	These findings suggest that the leaf extract from Alpinia zerumbet could be a candidate for the development of a drug to treat inflammation and ROS related disorders.
30544575	9	77	theme	anti-nociceptive	1374:1389	arg1	effects					1391:1397	both central and peripheral anti-nociceptive effects	1346:1397	both central and peripheral anti-nociceptive effects in mice	1346:1405	Additionally, the extract demonstrated both central and peripheral anti-nociceptive effects in mice, as manifested by a decrease in the count of writhing, induced with acetic acid and an increase in the latency time in the hot plate test.
29808415	6	0	theme	primary	903:909	arg1	cells					921:925	primary dendritic cells	903:925	primary dendritic cells	903:925	Here, we investigated whether immune-activating or -suppressing stimuli imposed on primary dendritic cells affected the release of various small non-coding RNAs via EV.
29808415	7	1	from	particles	1076:1084	arg1	free					1048:1051	free	1048:1051	free	1048:1051	The small RNA transcriptomes of highly pure EV populations free from ribonucleoprotein particles were analyzed by RNA sequencing and RT-qPCR.
29808415	9	2	theme	interpreting	1399:1410	arg1	EV					1412:1413	interpreting EV	1399:1413	interpreting EV	1399:1413	Only part of the changes in EV-RNA content reflected changes in cellular RNA, which urges caution in interpreting EV as snapshots of cells.
29808415	4	3	theme	RNAs	642:645	arg1	classes					614:620	multiple classes	605:620	multiple classes of small non-coding RNAs with potential gene-regulatory functions	605:686	Although research on EV-associated RNA has predominantly focused on microRNAs, the transcriptome of EV consists of multiple classes of small non-coding RNAs with potential gene-regulatory functions.
29808415	10	4	theme	multiple	1525:1532	arg1	classes					1538:1544	multiple RNA classes	1525:1544	multiple RNA classes	1525:1544	By comprehensive analysis of RNA obtained from highly purified EV, we demonstrate that multiple RNA classes contribute to genetic messages conveyed via EV.
29808415	11	5	theme	classes	1629:1635	arg1	identification					1598:1611	The identification	1594:1611	The identification of multiple RNA classes that display cell stimulation-dependent association with EV	1594:1695	The identification of multiple RNA classes that display cell stimulation-dependent association with EV is the prelude to unraveling the function and biomarker potential of these EV-RNAs.
29808415	11	5	theme	classes	1629:1635	arg1	prelude					1704:1710	the prelude	1700:1710	the prelude to unraveling the function and biomarker potential of these EV-RNAs	1700:1778	The identification of multiple RNA classes that display cell stimulation-dependent association with EV is the prelude to unraveling the function and biomarker potential of these EV-RNAs.
29808415	0	6	theme	Immune	0:5	arg1	stimuli					7:13	Immune stimuli	0:13	Immune stimuli	0:13	Immune stimuli shape the small non-coding transcriptome of extracellular vesicles released by dendritic cells.
29808415	9	7	from	part	1303:1306	arg1	content					1333:1339	EV-RNA content	1326:1339	EV-RNA content	1326:1339	Only part of the changes in EV-RNA content reflected changes in cellular RNA, which urges caution in interpreting EV as snapshots of cells.
29808415	8	8	from	content	1207:1213	arg1	cells					1236:1240	dendritic cells	1226:1240	dendritic cells	1226:1240	Immune stimulus-specific changes were found in the miRNA, snoRNA, and Y-RNA content of EV from dendritic cells, whereas tRNA and snRNA levels were much less affected.
29808415	4	9	theme	potential	652:660	arg1	functions					678:686	potential gene-regulatory functions	652:686	potential gene-regulatory functions	652:686	Although research on EV-associated RNA has predominantly focused on microRNAs, the transcriptome of EV consists of multiple classes of small non-coding RNAs with potential gene-regulatory functions.
29808415	8	10	theme	Y-RNA	1201:1205	arg1	content					1207:1213	the miRNA, snoRNA, and Y-RNA content	1178:1213	the miRNA, snoRNA, and Y-RNA content of EV from dendritic cells	1178:1240	Immune stimulus-specific changes were found in the miRNA, snoRNA, and Y-RNA content of EV from dendritic cells, whereas tRNA and snRNA levels were much less affected.
29808415	6	11	theme	RNAs	976:979	arg1	release					940:946	the release	936:946	the release of various small non-coding RNAs	936:979	Here, we investigated whether immune-activating or -suppressing stimuli imposed on primary dendritic cells affected the release of various small non-coding RNAs via EV.
29808415	11	12	theme	EV-RNAs	1772:1778	arg1	function					1730:1737	function	1730:1737	function	1730:1737	The identification of multiple RNA classes that display cell stimulation-dependent association with EV is the prelude to unraveling the function and biomarker potential of these EV-RNAs.
29808415	11	12	theme	EV-RNAs	1772:1778	arg1	potential					1753:1761	biomarker potential	1743:1761	biomarker potential	1743:1761	The identification of multiple RNA classes that display cell stimulation-dependent association with EV is the prelude to unraveling the function and biomarker potential of these EV-RNAs.
29808415	7	13	theme	RNA	999:1001	arg1	transcriptomes					1003:1016	The small RNA transcriptomes	989:1016	The small RNA transcriptomes of highly pure EV populations free from ribonucleoprotein particles	989:1084	The small RNA transcriptomes of highly pure EV populations free from ribonucleoprotein particles were analyzed by RNA sequencing and RT-qPCR.
29808415	2	14	theme	signaling	287:295	arg1	function					297:304	their signaling function	281:304	their signaling function to target cells	281:320	The molecular composition of EV, and thereby their signaling function to target cells, is regulated by cellular activation and differentiation stimuli.
29808415	1	15	theme	conserved	188:196	arg1	uptake					127:132	uptake	127:132	uptake	127:132	The release and uptake of nano-sized extracellular vesicles (EV) is a highly conserved means of intercellular communication.
29808415	1	15	theme	conserved	188:196	arg1	release					115:121	release	115:121	release	115:121	The release and uptake of nano-sized extracellular vesicles (EV) is a highly conserved means of intercellular communication.
29808415	1	15	theme	conserved	188:196	arg1	means					198:202	a highly conserved means	179:202	a highly conserved means of intercellular communication	179:233	The release and uptake of nano-sized extracellular vesicles (EV) is a highly conserved means of intercellular communication.
29808415	6	16	theme	small	959:963	arg1	RNAs					976:979	various small non-coding RNAs	951:979	various small non-coding RNAs	951:979	Here, we investigated whether immune-activating or -suppressing stimuli imposed on primary dendritic cells affected the release of various small non-coding RNAs via EV.
29808415	2	17	theme	differentiation	363:377	arg1	stimuli					379:385	differentiation stimuli	363:385	differentiation stimuli	363:385	The molecular composition of EV, and thereby their signaling function to target cells, is regulated by cellular activation and differentiation stimuli.
29808415	4	18	theme	small	625:629	arg1	RNAs					642:645	small non-coding RNAs	625:645	small non-coding RNAs with potential gene-regulatory functions	625:686	Although research on EV-associated RNA has predominantly focused on microRNAs, the transcriptome of EV consists of multiple classes of small non-coding RNAs with potential gene-regulatory functions.
29808415	7	19	theme	ribonucleoprotein	1058:1074	arg1	particles					1076:1084	ribonucleoprotein particles	1058:1084	ribonucleoprotein particles	1058:1084	The small RNA transcriptomes of highly pure EV populations free from ribonucleoprotein particles were analyzed by RNA sequencing and RT-qPCR.
29808415	11	20	dep	function	1730:1737	arg1	the					1726:1728	the	1726:1728	the	1726:1728	The identification of multiple RNA classes that display cell stimulation-dependent association with EV is the prelude to unraveling the function and biomarker potential of these EV-RNAs.
29808415	7	21	theme	free	1048:1051	arg1	populations					1036:1046	highly pure EV populations	1021:1046	highly pure EV populations free from ribonucleoprotein particles	1021:1084	The small RNA transcriptomes of highly pure EV populations free from ribonucleoprotein particles were analyzed by RNA sequencing and RT-qPCR.
29808415	8	22	from	cells	1236:1240	arg1	EV					1218:1219	EV	1218:1219	EV from dendritic cells	1218:1240	Immune stimulus-specific changes were found in the miRNA, snoRNA, and Y-RNA content of EV from dendritic cells, whereas tRNA and snRNA levels were much less affected.
29808415	8	22	from	cells	1236:1240	arg1	content					1207:1213	the miRNA, snoRNA, and Y-RNA content	1178:1213	the miRNA, snoRNA, and Y-RNA content of EV from dendritic cells	1178:1240	Immune stimulus-specific changes were found in the miRNA, snoRNA, and Y-RNA content of EV from dendritic cells, whereas tRNA and snRNA levels were much less affected.
29808415	7	23	theme	EV	1033:1034	arg1	populations					1036:1046	highly pure EV populations	1021:1046	highly pure EV populations free from ribonucleoprotein particles	1021:1084	The small RNA transcriptomes of highly pure EV populations free from ribonucleoprotein particles were analyzed by RNA sequencing and RT-qPCR.
29808415	0	24	theme	extracellular	59:71	arg1	vesicles					73:80	extracellular vesicles	59:80	extracellular vesicles released by dendritic cells	59:108	Immune stimuli shape the small non-coding transcriptome of extracellular vesicles released by dendritic cells.
29808415	2	25	theme	molecular	240:248	arg1	composition					250:260	The molecular composition	236:260	The molecular composition of EV	236:266	The molecular composition of EV, and thereby their signaling function to target cells, is regulated by cellular activation and differentiation stimuli.
29808415	1	26	theme	extracellular	148:160	arg1	EV					172:173	EV	172:173	EV	172:173	The release and uptake of nano-sized extracellular vesicles (EV) is a highly conserved means of intercellular communication.
29808415	1	26	theme	extracellular	148:160	arg1	vesicles					162:169	nano-sized extracellular vesicles	137:169	nano-sized extracellular vesicles (EV)	137:174	The release and uptake of nano-sized extracellular vesicles (EV) is a highly conserved means of intercellular communication.
29808415	11	27	theme	biomarker	1743:1751	arg1	potential					1753:1761	biomarker potential	1743:1761	biomarker potential	1743:1761	The identification of multiple RNA classes that display cell stimulation-dependent association with EV is the prelude to unraveling the function and biomarker potential of these EV-RNAs.
29808415	0	28	theme	dendritic	94:102	arg1	cells					104:108	dendritic cells	94:108	dendritic cells	94:108	Immune stimuli shape the small non-coding transcriptome of extracellular vesicles released by dendritic cells.
29808415	10	29	theme	comprehensive	1441:1453	arg1	analysis					1455:1462	comprehensive analysis	1441:1462	comprehensive analysis of RNA obtained from highly purified EV	1441:1502	By comprehensive analysis of RNA obtained from highly purified EV, we demonstrate that multiple RNA classes contribute to genetic messages conveyed via EV.
29808415	1	30	dep	release	115:121	arg1	The					111:113	The	111:113	The	111:113	The release and uptake of nano-sized extracellular vesicles (EV) is a highly conserved means of intercellular communication.
29808415	5	31	theme	EV-associated	793:805	arg1	classes					811:817	EV-associated RNA classes	793:817	EV-associated RNA classes	793:817	It is not known whether environmental cues imposed on cells induce specific changes in a broad range of EV-associated RNA classes.
29808415	9	32	from	changes	1315:1321	arg1	content					1333:1339	EV-RNA content	1326:1339	EV-RNA content	1326:1339	Only part of the changes in EV-RNA content reflected changes in cellular RNA, which urges caution in interpreting EV as snapshots of cells.
29808415	5	33	theme	classes	811:817	arg1	range					784:788	a broad range	776:788	a broad range of EV-associated RNA classes	776:817	It is not known whether environmental cues imposed on cells induce specific changes in a broad range of EV-associated RNA classes.
29808415	0	34	theme	small	25:29	arg1	transcriptome					42:54	the small non-coding transcriptome	21:54	the small non-coding transcriptome of extracellular vesicles released by dendritic cells	21:108	Immune stimuli shape the small non-coding transcriptome of extracellular vesicles released by dendritic cells.
29808415	9	35	from	caution	1388:1394	arg1	EV					1412:1413	interpreting EV	1399:1413	interpreting EV	1399:1413	Only part of the changes in EV-RNA content reflected changes in cellular RNA, which urges caution in interpreting EV as snapshots of cells.
29808415	4	36	from	research	499:506	arg1	RNA					525:527	EV-associated RNA	511:527	EV-associated RNA	511:527	Although research on EV-associated RNA has predominantly focused on microRNAs, the transcriptome of EV consists of multiple classes of small non-coding RNAs with potential gene-regulatory functions.
29808415	11	37	theme	stimulation-dependent	1655:1675	arg1	association					1677:1687	cell stimulation-dependent association	1650:1687	cell stimulation-dependent association with EV	1650:1695	The identification of multiple RNA classes that display cell stimulation-dependent association with EV is the prelude to unraveling the function and biomarker potential of these EV-RNAs.
29808415	4	38	theme	EV-associated	511:523	arg1	RNA					525:527	EV-associated RNA	511:527	EV-associated RNA	511:527	Although research on EV-associated RNA has predominantly focused on microRNAs, the transcriptome of EV consists of multiple classes of small non-coding RNAs with potential gene-regulatory functions.
29808415	9	39	theme	cellular	1362:1369	arg1	RNA					1371:1373	cellular RNA	1362:1373	cellular RNA	1362:1373	Only part of the changes in EV-RNA content reflected changes in cellular RNA, which urges caution in interpreting EV as snapshots of cells.
29808415	8	40	theme	dendritic	1226:1234	arg1	cells					1236:1240	dendritic cells	1226:1240	dendritic cells	1226:1240	Immune stimulus-specific changes were found in the miRNA, snoRNA, and Y-RNA content of EV from dendritic cells, whereas tRNA and snRNA levels were much less affected.
29808415	8	41	theme	stimulus-specific	1138:1154	arg1	changes					1156:1162	Immune stimulus-specific changes	1131:1162	Immune stimulus-specific changes	1131:1162	Immune stimulus-specific changes were found in the miRNA, snoRNA, and Y-RNA content of EV from dendritic cells, whereas tRNA and snRNA levels were much less affected.
29808415	11	42	theme	RNA	1625:1627	arg1	classes					1629:1635	multiple RNA classes	1616:1635	multiple RNA classes that display cell stimulation-dependent association with EV	1616:1695	The identification of multiple RNA classes that display cell stimulation-dependent association with EV is the prelude to unraveling the function and biomarker potential of these EV-RNAs.
29808415	6	43	theme	-suppressing	871:882	arg1	stimuli					884:890	immune-activating or -suppressing stimuli	850:890	immune-activating or -suppressing stimuli imposed on primary dendritic cells	850:925	Here, we investigated whether immune-activating or -suppressing stimuli imposed on primary dendritic cells affected the release of various small non-coding RNAs via EV.
29808415	2	44	theme	cellular	339:346	arg1	activation					348:357	cellular activation	339:357	cellular activation	339:357	The molecular composition of EV, and thereby their signaling function to target cells, is regulated by cellular activation and differentiation stimuli.
29808415	6	45	theme	dendritic	911:919	arg1	cells					921:925	primary dendritic cells	903:925	primary dendritic cells	903:925	Here, we investigated whether immune-activating or -suppressing stimuli imposed on primary dendritic cells affected the release of various small non-coding RNAs via EV.
29808415	10	46	theme	RNA	1534:1536	arg1	classes					1538:1544	multiple RNA classes	1525:1544	multiple RNA classes	1525:1544	By comprehensive analysis of RNA obtained from highly purified EV, we demonstrate that multiple RNA classes contribute to genetic messages conveyed via EV.
29808415	11	47	theme	cell	1650:1653	arg1	association					1677:1687	cell stimulation-dependent association	1650:1687	cell stimulation-dependent association with EV	1650:1695	The identification of multiple RNA classes that display cell stimulation-dependent association with EV is the prelude to unraveling the function and biomarker potential of these EV-RNAs.
29808415	7	48	theme	RNA	1103:1105	arg1	sequencing					1107:1116	RNA sequencing	1103:1116	RNA sequencing	1103:1116	The small RNA transcriptomes of highly pure EV populations free from ribonucleoprotein particles were analyzed by RNA sequencing and RT-qPCR.
29808415	8	49	theme	miRNA	1182:1186	arg1	content					1207:1213	the miRNA, snoRNA, and Y-RNA content	1178:1213	the miRNA, snoRNA, and Y-RNA content of EV from dendritic cells	1178:1240	Immune stimulus-specific changes were found in the miRNA, snoRNA, and Y-RNA content of EV from dendritic cells, whereas tRNA and snRNA levels were much less affected.
29808415	4	50	theme	non-coding	631:640	arg1	RNAs					642:645	small non-coding RNAs	625:645	small non-coding RNAs with potential gene-regulatory functions	625:686	Although research on EV-associated RNA has predominantly focused on microRNAs, the transcriptome of EV consists of multiple classes of small non-coding RNAs with potential gene-regulatory functions.
29808415	2	51	theme	target	309:314	arg1	cells					316:320	target cells	309:320	target cells	309:320	The molecular composition of EV, and thereby their signaling function to target cells, is regulated by cellular activation and differentiation stimuli.
29808415	8	52	theme	snoRNA	1189:1194	arg1	content					1207:1213	the miRNA, snoRNA, and Y-RNA content	1178:1213	the miRNA, snoRNA, and Y-RNA content of EV from dendritic cells	1178:1240	Immune stimulus-specific changes were found in the miRNA, snoRNA, and Y-RNA content of EV from dendritic cells, whereas tRNA and snRNA levels were much less affected.
29808415	10	53	theme	genetic	1560:1566	arg1	messages					1568:1575	genetic messages	1560:1575	genetic messages conveyed via EV	1560:1591	By comprehensive analysis of RNA obtained from highly purified EV, we demonstrate that multiple RNA classes contribute to genetic messages conveyed via EV.
29808415	1	54	theme	intercellular	207:219	arg1	communication					221:233	intercellular communication	207:233	intercellular communication	207:233	The release and uptake of nano-sized extracellular vesicles (EV) is a highly conserved means of intercellular communication.
29808415	11	55	with	association	1677:1687	arg1	EV					1694:1695	EV	1694:1695	EV	1694:1695	The identification of multiple RNA classes that display cell stimulation-dependent association with EV is the prelude to unraveling the function and biomarker potential of these EV-RNAs.
29808415	9	56	theme	cells	1431:1435	arg1	snapshots					1418:1426	snapshots	1418:1426	snapshots of cells	1418:1435	Only part of the changes in EV-RNA content reflected changes in cellular RNA, which urges caution in interpreting EV as snapshots of cells.
29808415	9	56	theme	cells	1431:1435	arg1	caution					1388:1394	caution	1388:1394	caution in interpreting EV	1388:1413	Only part of the changes in EV-RNA content reflected changes in cellular RNA, which urges caution in interpreting EV as snapshots of cells.
29808415	6	57	theme	non-coding	965:974	arg1	RNAs					976:979	various small non-coding RNAs	951:979	various small non-coding RNAs	951:979	Here, we investigated whether immune-activating or -suppressing stimuli imposed on primary dendritic cells affected the release of various small non-coding RNAs via EV.
29808415	4	58	theme	multiple	605:612	arg1	classes					614:620	multiple classes	605:620	multiple classes of small non-coding RNAs with potential gene-regulatory functions	605:686	Although research on EV-associated RNA has predominantly focused on microRNAs, the transcriptome of EV consists of multiple classes of small non-coding RNAs with potential gene-regulatory functions.
29808415	6	59	theme	various	951:957	arg1	RNAs					976:979	various small non-coding RNAs	951:979	various small non-coding RNAs	951:979	Here, we investigated whether immune-activating or -suppressing stimuli imposed on primary dendritic cells affected the release of various small non-coding RNAs via EV.
29808415	8	60	theme	snRNA	1260:1264	arg1	levels					1266:1271	tRNA and snRNA levels	1251:1271	tRNA and snRNA levels	1251:1271	Immune stimulus-specific changes were found in the miRNA, snoRNA, and Y-RNA content of EV from dendritic cells, whereas tRNA and snRNA levels were much less affected.
29808415	7	61	theme	populations	1036:1046	arg1	transcriptomes					1003:1016	The small RNA transcriptomes	989:1016	The small RNA transcriptomes of highly pure EV populations free from ribonucleoprotein particles	989:1084	The small RNA transcriptomes of highly pure EV populations free from ribonucleoprotein particles were analyzed by RNA sequencing and RT-qPCR.
29808415	8	62	theme	EV	1218:1219	arg1	content					1207:1213	the miRNA, snoRNA, and Y-RNA content	1178:1213	the miRNA, snoRNA, and Y-RNA content of EV from dendritic cells	1178:1240	Immune stimulus-specific changes were found in the miRNA, snoRNA, and Y-RNA content of EV from dendritic cells, whereas tRNA and snRNA levels were much less affected.
29808415	7	63	theme	pure	1028:1031	arg1	populations					1036:1046	highly pure EV populations	1021:1046	highly pure EV populations free from ribonucleoprotein particles	1021:1084	The small RNA transcriptomes of highly pure EV populations free from ribonucleoprotein particles were analyzed by RNA sequencing and RT-qPCR.
29808415	2	64	theme	EV	265:266	arg1	composition					250:260	The molecular composition	236:260	The molecular composition of EV	236:266	The molecular composition of EV, and thereby their signaling function to target cells, is regulated by cellular activation and differentiation stimuli.
29808415	0	65	theme	vesicles	73:80	arg1	transcriptome					42:54	the small non-coding transcriptome	21:54	the small non-coding transcriptome of extracellular vesicles released by dendritic cells	21:108	Immune stimuli shape the small non-coding transcriptome of extracellular vesicles released by dendritic cells.
29808415	7	66	theme	small	993:997	arg1	transcriptomes					1003:1016	The small RNA transcriptomes	989:1016	The small RNA transcriptomes of highly pure EV populations free from ribonucleoprotein particles	989:1084	The small RNA transcriptomes of highly pure EV populations free from ribonucleoprotein particles were analyzed by RNA sequencing and RT-qPCR.
29808415	1	67	theme	nano-sized	137:146	arg1	EV					172:173	EV	172:173	EV	172:173	The release and uptake of nano-sized extracellular vesicles (EV) is a highly conserved means of intercellular communication.
29808415	1	67	theme	nano-sized	137:146	arg1	vesicles					162:169	nano-sized extracellular vesicles	137:169	nano-sized extracellular vesicles (EV)	137:174	The release and uptake of nano-sized extracellular vesicles (EV) is a highly conserved means of intercellular communication.
29808415	4	68	theme	gene-regulatory	662:676	arg1	functions					678:686	potential gene-regulatory functions	652:686	potential gene-regulatory functions	652:686	Although research on EV-associated RNA has predominantly focused on microRNAs, the transcriptome of EV consists of multiple classes of small non-coding RNAs with potential gene-regulatory functions.
29808415	5	69	theme	specific	756:763	arg1	changes					765:771	specific changes	756:771	specific changes	756:771	It is not known whether environmental cues imposed on cells induce specific changes in a broad range of EV-associated RNA classes.
29808415	9	70	from	changes	1351:1357	arg1	RNA					1371:1373	cellular RNA	1362:1373	cellular RNA	1362:1373	Only part of the changes in EV-RNA content reflected changes in cellular RNA, which urges caution in interpreting EV as snapshots of cells.
29808415	1	71	theme	vesicles	162:169	arg1	uptake					127:132	uptake	127:132	uptake	127:132	The release and uptake of nano-sized extracellular vesicles (EV) is a highly conserved means of intercellular communication.
29808415	1	71	theme	vesicles	162:169	arg1	release					115:121	release	115:121	release	115:121	The release and uptake of nano-sized extracellular vesicles (EV) is a highly conserved means of intercellular communication.
29808415	1	71	theme	vesicles	162:169	arg1	means					198:202	a highly conserved means	179:202	a highly conserved means of intercellular communication	179:233	The release and uptake of nano-sized extracellular vesicles (EV) is a highly conserved means of intercellular communication.
29808415	5	72	theme	environmental	713:725	arg1	cues					727:730	environmental cues	713:730	environmental cues imposed on cells	713:747	It is not known whether environmental cues imposed on cells induce specific changes in a broad range of EV-associated RNA classes.
29808415	9	73	from	content	1333:1339	arg1	part					1303:1306	Only part	1298:1306	Only part of the changes in EV-RNA content	1298:1339	Only part of the changes in EV-RNA content reflected changes in cellular RNA, which urges caution in interpreting EV as snapshots of cells.
29808415	8	74	theme	tRNA	1251:1254	arg1	levels					1266:1271	tRNA and snRNA levels	1251:1271	tRNA and snRNA levels	1251:1271	Immune stimulus-specific changes were found in the miRNA, snoRNA, and Y-RNA content of EV from dendritic cells, whereas tRNA and snRNA levels were much less affected.
29808415	8	75	located	found	1169:1173	arg2	changes					1156:1162	Immune stimulus-specific changes	1131:1162	Immune stimulus-specific changes	1131:1162	Immune stimulus-specific changes were found in the miRNA, snoRNA, and Y-RNA content of EV from dendritic cells, whereas tRNA and snRNA levels were much less affected.
29808415	8	75	located	found	1169:1173	arg1	content					1207:1213	the miRNA, snoRNA, and Y-RNA content	1178:1213	the miRNA, snoRNA, and Y-RNA content of EV from dendritic cells	1178:1240	Immune stimulus-specific changes were found in the miRNA, snoRNA, and Y-RNA content of EV from dendritic cells, whereas tRNA and snRNA levels were much less affected.
29808415	8	76	dep	found	1169:1173	arg1	whereas					1243:1249	whereas	1243:1249	whereas	1243:1249	Immune stimulus-specific changes were found in the miRNA, snoRNA, and Y-RNA content of EV from dendritic cells, whereas tRNA and snRNA levels were much less affected.
29808415	3	77	theme	cells	420:424	arg1	EV					388:389	EV	388:389	EV	388:389	EV are regarded as snapshots of cells and are, therefore, in the limelight as biomarkers for disease.
29808415	3	77	theme	cells	420:424	arg1	snapshots					407:415	snapshots	407:415	snapshots of cells	407:424	EV are regarded as snapshots of cells and are, therefore, in the limelight as biomarkers for disease.
29808415	5	78	theme	RNA	807:809	arg1	classes					811:817	EV-associated RNA classes	793:817	EV-associated RNA classes	793:817	It is not known whether environmental cues imposed on cells induce specific changes in a broad range of EV-associated RNA classes.
29808415	9	79	theme	changes	1315:1321	arg1	part					1303:1306	Only part	1298:1306	Only part of the changes in EV-RNA content	1298:1339	Only part of the changes in EV-RNA content reflected changes in cellular RNA, which urges caution in interpreting EV as snapshots of cells.
29808415	10	80	theme	RNA	1467:1469	arg1	analysis					1455:1462	comprehensive analysis	1441:1462	comprehensive analysis of RNA obtained from highly purified EV	1441:1502	By comprehensive analysis of RNA obtained from highly purified EV, we demonstrate that multiple RNA classes contribute to genetic messages conveyed via EV.
29808415	0	81	theme	non-coding	31:40	arg1	transcriptome					42:54	the small non-coding transcriptome	21:54	the small non-coding transcriptome of extracellular vesicles released by dendritic cells	21:108	Immune stimuli shape the small non-coding transcriptome of extracellular vesicles released by dendritic cells.
29808415	9	82	theme	EV-RNA	1326:1331	arg1	content					1333:1339	EV-RNA content	1326:1339	EV-RNA content	1326:1339	Only part of the changes in EV-RNA content reflected changes in cellular RNA, which urges caution in interpreting EV as snapshots of cells.
29808415	6	83	theme	immune-activating	850:866	arg1	stimuli					884:890	immune-activating or -suppressing stimuli	850:890	immune-activating or -suppressing stimuli imposed on primary dendritic cells	850:925	Here, we investigated whether immune-activating or -suppressing stimuli imposed on primary dendritic cells affected the release of various small non-coding RNAs via EV.
29808415	4	84	with	RNAs	642:645	arg1	functions					678:686	potential gene-regulatory functions	652:686	potential gene-regulatory functions	652:686	Although research on EV-associated RNA has predominantly focused on microRNAs, the transcriptome of EV consists of multiple classes of small non-coding RNAs with potential gene-regulatory functions.
29808415	5	85	theme	broad	778:782	arg1	range					784:788	a broad range	776:788	a broad range of EV-associated RNA classes	776:817	It is not known whether environmental cues imposed on cells induce specific changes in a broad range of EV-associated RNA classes.
29808415	10	86	theme	purified	1492:1499	arg1	EV					1501:1502	highly purified EV	1485:1502	highly purified EV	1485:1502	By comprehensive analysis of RNA obtained from highly purified EV, we demonstrate that multiple RNA classes contribute to genetic messages conveyed via EV.
29808415	8	87	theme	Immune	1131:1136	arg1	changes					1156:1162	Immune stimulus-specific changes	1131:1162	Immune stimulus-specific changes	1131:1162	Immune stimulus-specific changes were found in the miRNA, snoRNA, and Y-RNA content of EV from dendritic cells, whereas tRNA and snRNA levels were much less affected.
29808415	4	88	theme	EV	590:591	arg1	transcriptome					573:585	the transcriptome	569:585	the transcriptome of EV	569:591	Although research on EV-associated RNA has predominantly focused on microRNAs, the transcriptome of EV consists of multiple classes of small non-coding RNAs with potential gene-regulatory functions.
29808415	1	89	theme	communication	221:233	arg1	uptake					127:132	uptake	127:132	uptake	127:132	The release and uptake of nano-sized extracellular vesicles (EV) is a highly conserved means of intercellular communication.
29808415	1	89	theme	communication	221:233	arg1	release					115:121	release	115:121	release	115:121	The release and uptake of nano-sized extracellular vesicles (EV) is a highly conserved means of intercellular communication.
29808415	1	89	theme	communication	221:233	arg1	means					198:202	a highly conserved means	179:202	a highly conserved means of intercellular communication	179:233	The release and uptake of nano-sized extracellular vesicles (EV) is a highly conserved means of intercellular communication.
29808415	11	90	theme	multiple	1616:1623	arg1	classes					1629:1635	multiple RNA classes	1616:1635	multiple RNA classes that display cell stimulation-dependent association with EV	1616:1695	The identification of multiple RNA classes that display cell stimulation-dependent association with EV is the prelude to unraveling the function and biomarker potential of these EV-RNAs.
30717392	12	0	theme	gut-associated	1431:1444	arg1	tissue					1455:1460	the gut-associated lymphoid tissue	1427:1460	the gut-associated lymphoid tissue	1427:1460	These results show the immunomodulatory actions of hesperidin on the gut-associated lymphoid tissue and reinforce its role as a prebiotic.
30717392	9	1	theme	small	1110:1114	arg1	content					1130:1136	the small intestine IgA content	1106:1136	the small intestine IgA content	1106:1136	Hesperidin was also able to increase the small intestine IgA content.
30717392	0	2	theme	Healthy	75:81	arg1	Rats					83:86	Healthy Rats	75:86	Healthy Rats	75:86	Hesperidin Effects on Gut Microbiota and Gut-Associated Lymphoid Tissue in Healthy Rats.
30717392	0	3	from	Effects	11:17	arg1	Tissue					65:70	Gut-Associated Lymphoid Tissue	41:70	Gut-Associated Lymphoid Tissue	41:70	Hesperidin Effects on Gut Microbiota and Gut-Associated Lymphoid Tissue in Healthy Rats.
30717392	0	3	from	Effects	11:17	arg1	Microbiota					26:35	Gut Microbiota	22:35	Gut Microbiota	22:35	Hesperidin Effects on Gut Microbiota and Gut-Associated Lymphoid Tissue in Healthy Rats.
30717392	11	4	theme	lymphocytes	1341:1351	arg1	proportion					1320:1329	the relative proportion	1307:1329	the relative proportion of TCRαβ+ lymphocytes in MLNL	1307:1359	In addition, hesperidin increased the relative proportion of TCRαβ+ lymphocytes in MLNL.
30717392	1	5	theme	wide	137:140	arg1	range					142:146	a wide range	135:146	a wide range of biological properties	135:171	Hesperidin, found in citrus fruits, has shown a wide range of biological properties.
30717392	10	6	theme	monocyte	1227:1234	arg1	protein-1					1248:1256	monocyte chemotactic protein-1	1227:1256	monocyte chemotactic protein-1 concentration	1227:1270	These changes in the small intestine were accompanied by a decrease in interferon-γ and monocyte chemotactic protein-1 concentration.
30717392	5	7	theme	IgA-coated	682:691	arg1	bacteria					693:700	IgA-coated bacteria	682:700	IgA-coated bacteria	682:700	Microbiota composition and IgA-coated bacteria were determined in caecal content.
30717392	2	8	with	relationship	342:353	arg1	microbiota					368:377	the gut microbiota	360:377	the gut microbiota	360:377	Nonetheless, a more in-depth investigation is required on the effects on the immune system, and in particular, on the gut-associated lymphoid tissue, together with its relationship with the gut microbiota.
30717392	2	9	theme	lymphoid	307:314	arg1	tissue					316:321	the gut-associated lymphoid tissue	288:321	the gut-associated lymphoid tissue	288:321	Nonetheless, a more in-depth investigation is required on the effects on the immune system, and in particular, on the gut-associated lymphoid tissue, together with its relationship with the gut microbiota.
30717392	3	10	theme	healthy	536:542	arg1	animals					544:550	healthy animals	536:550	healthy animals	536:550	Therefore, we aimed to establish the influence of oral hesperidin administration on the intestinal lymphoid tissue and on the gut microbiota composition in healthy animals.
30717392	8	11	theme	microbiota	1005:1014	arg1	composition					1016:1026	microbiota composition	1005:1026	microbiota composition	1005:1026	Hesperidin administration resulted in a higher number of bacteria and IgA-coated bacteria, with changes in microbiota composition such as higher Lactobacillus proportion.
30717392	4	12	theme	Lewis	553:557	arg1	rats					559:562	Lewis rats	553:562	Lewis rats	553:562	Lewis rats were orally administrated 100 or 200 mg/kg hesperidin three times per week for four weeks.
30717392	12	13	theme	immunomodulatory	1385:1400	arg1	actions					1402:1408	the immunomodulatory actions	1381:1408	the immunomodulatory actions of hesperidin on the gut-associated lymphoid tissue	1381:1460	These results show the immunomodulatory actions of hesperidin on the gut-associated lymphoid tissue and reinforce its role as a prebiotic.
30717392	8	14	theme	bacteria	955:962	arg1	number					945:950	a higher number	936:950	a higher number of bacteria and IgA-coated bacteria	936:986	Hesperidin administration resulted in a higher number of bacteria and IgA-coated bacteria, with changes in microbiota composition such as higher Lactobacillus proportion.
30717392	2	15	theme	immune	251:256	arg1	system					258:263	the immune system	247:263	the immune system	247:263	Nonetheless, a more in-depth investigation is required on the effects on the immune system, and in particular, on the gut-associated lymphoid tissue, together with its relationship with the gut microbiota.
30717392	2	16	theme	gut-associated	292:305	arg1	tissue					316:321	the gut-associated lymphoid tissue	288:321	the gut-associated lymphoid tissue	288:321	Nonetheless, a more in-depth investigation is required on the effects on the immune system, and in particular, on the gut-associated lymphoid tissue, together with its relationship with the gut microbiota.
30717392	10	17	theme	chemotactic	1236:1246	arg1	protein-1					1248:1256	monocyte chemotactic protein-1	1227:1256	monocyte chemotactic protein-1 concentration	1227:1270	These changes in the small intestine were accompanied by a decrease in interferon-γ and monocyte chemotactic protein-1 concentration.
30717392	12	18	theme	hesperidin	1413:1422	arg1	actions					1402:1408	the immunomodulatory actions	1381:1408	the immunomodulatory actions of hesperidin on the gut-associated lymphoid tissue	1381:1460	These results show the immunomodulatory actions of hesperidin on the gut-associated lymphoid tissue and reinforce its role as a prebiotic.
30717392	12	19	theme	lymphoid	1446:1453	arg1	tissue					1455:1460	the gut-associated lymphoid tissue	1427:1460	the gut-associated lymphoid tissue	1427:1460	These results show the immunomodulatory actions of hesperidin on the gut-associated lymphoid tissue and reinforce its role as a prebiotic.
30717392	6	20	theme	lymphocyte	759:768	arg1	composition					777:787	Mesenteric lymph node lymphocyte (MLNL) composition	737:787	Mesenteric lymph node lymphocyte (MLNL) composition	737:787	Mesenteric lymph node lymphocyte (MLNL) composition and functionality were assessed.
30717392	7	21	from	expression	847:856	arg1	intestine					871:879	the small intestine	861:879	the small intestine	861:879	IgA, cytokines, and gene expression in the small intestine were quantified.
30717392	12	22	from	actions	1402:1408	arg1	tissue					1455:1460	the gut-associated lymphoid tissue	1427:1460	the gut-associated lymphoid tissue	1427:1460	These results show the immunomodulatory actions of hesperidin on the gut-associated lymphoid tissue and reinforce its role as a prebiotic.
30717392	4	23	theme	hesperidin	607:616	arg1	times					624:628	100 or 200 mg/kg hesperidin three times	590:628	100 or 200 mg/kg hesperidin three times	590:628	Lewis rats were orally administrated 100 or 200 mg/kg hesperidin three times per week for four weeks.
30717392	0	24	from	Microbiota	26:35	arg1	Rats					83:86	Healthy Rats	75:86	Healthy Rats	75:86	Hesperidin Effects on Gut Microbiota and Gut-Associated Lymphoid Tissue in Healthy Rats.
30717392	8	25	theme	higher	1036:1041	arg1	proportion					1057:1066	higher Lactobacillus proportion	1036:1066	higher Lactobacillus proportion	1036:1066	Hesperidin administration resulted in a higher number of bacteria and IgA-coated bacteria, with changes in microbiota composition such as higher Lactobacillus proportion.
30717392	6	26	theme	node	754:757	arg1	MLNL					771:774	MLNL	771:774	MLNL	771:774	Mesenteric lymph node lymphocyte (MLNL) composition and functionality were assessed.
30717392	6	26	theme	node	754:757	arg1	lymphocyte					759:768	Mesenteric lymph node lymphocyte	737:768	Mesenteric lymph node lymphocyte (MLNL) composition	737:787	Mesenteric lymph node lymphocyte (MLNL) composition and functionality were assessed.
30717392	11	27	from	proportion	1320:1329	arg1	MLNL					1356:1359	MLNL	1356:1359	MLNL	1356:1359	In addition, hesperidin increased the relative proportion of TCRαβ+ lymphocytes in MLNL.
30717392	1	28	theme	biological	151:160	arg1	properties					162:171	biological properties	151:171	biological properties	151:171	Hesperidin, found in citrus fruits, has shown a wide range of biological properties.
30717392	11	29	theme	TCRαβ+	1334:1339	arg1	lymphocytes					1341:1351	TCRαβ+ lymphocytes	1334:1351	TCRαβ+ lymphocytes	1334:1351	In addition, hesperidin increased the relative proportion of TCRαβ+ lymphocytes in MLNL.
30717392	6	30	theme	lymph	748:752	arg1	node					754:757	Mesenteric lymph node	737:757	Mesenteric lymph node lymphocyte (MLNL) composition	737:787	Mesenteric lymph node lymphocyte (MLNL) composition and functionality were assessed.
30717392	1	31	theme	properties	162:171	arg1	range					142:146	a wide range	135:146	a wide range of biological properties	135:171	Hesperidin, found in citrus fruits, has shown a wide range of biological properties.
30717392	0	32	theme	Gut	22:24	arg1	Microbiota					26:35	Gut Microbiota	22:35	Gut Microbiota	22:35	Hesperidin Effects on Gut Microbiota and Gut-Associated Lymphoid Tissue in Healthy Rats.
30717392	6	33	theme	Mesenteric	737:746	arg1	node					754:757	Mesenteric lymph node	737:757	Mesenteric lymph node lymphocyte (MLNL) composition	737:787	Mesenteric lymph node lymphocyte (MLNL) composition and functionality were assessed.
30717392	3	34	theme	gut	506:508	arg1	composition					521:531	the gut microbiota composition	502:531	the gut microbiota composition in healthy animals	502:550	Therefore, we aimed to establish the influence of oral hesperidin administration on the intestinal lymphoid tissue and on the gut microbiota composition in healthy animals.
30717392	8	35	from	changes	994:1000	arg1	composition					1016:1026	microbiota composition	1005:1026	microbiota composition	1005:1026	Hesperidin administration resulted in a higher number of bacteria and IgA-coated bacteria, with changes in microbiota composition such as higher Lactobacillus proportion.
30717392	5	36	theme	Microbiota	655:664	arg1	composition					666:676	Microbiota composition	655:676	Microbiota composition	655:676	Microbiota composition and IgA-coated bacteria were determined in caecal content.
30717392	3	37	theme	microbiota	510:519	arg1	composition					521:531	the gut microbiota composition	502:531	the gut microbiota composition in healthy animals	502:550	Therefore, we aimed to establish the influence of oral hesperidin administration on the intestinal lymphoid tissue and on the gut microbiota composition in healthy animals.
30717392	2	38	from	effects	236:242	arg1	system					258:263	the immune system	247:263	the immune system	247:263	Nonetheless, a more in-depth investigation is required on the effects on the immune system, and in particular, on the gut-associated lymphoid tissue, together with its relationship with the gut microbiota.
30717392	10	39	from	changes	1145:1151	arg1	intestine					1166:1174	the small intestine	1156:1174	the small intestine	1156:1174	These changes in the small intestine were accompanied by a decrease in interferon-γ and monocyte chemotactic protein-1 concentration.
30717392	3	40	theme	administration	446:459	arg1	influence					417:425	the influence	413:425	the influence of oral hesperidin administration on the intestinal lymphoid tissue and on the gut microbiota composition in healthy animals	413:550	Therefore, we aimed to establish the influence of oral hesperidin administration on the intestinal lymphoid tissue and on the gut microbiota composition in healthy animals.
30717392	4	41	theme	mg/kg	601:605	arg1	times					624:628	100 or 200 mg/kg hesperidin three times	590:628	100 or 200 mg/kg hesperidin three times	590:628	Lewis rats were orally administrated 100 or 200 mg/kg hesperidin three times per week for four weeks.
30717392	8	42	theme	Lactobacillus	1043:1055	arg1	proportion					1057:1066	higher Lactobacillus proportion	1036:1066	higher Lactobacillus proportion	1036:1066	Hesperidin administration resulted in a higher number of bacteria and IgA-coated bacteria, with changes in microbiota composition such as higher Lactobacillus proportion.
30717392	2	43	theme	gut	364:366	arg1	microbiota					368:377	the gut microbiota	360:377	the gut microbiota	360:377	Nonetheless, a more in-depth investigation is required on the effects on the immune system, and in particular, on the gut-associated lymphoid tissue, together with its relationship with the gut microbiota.
30717392	11	44	theme	relative	1311:1318	arg1	proportion					1320:1329	the relative proportion	1307:1329	the relative proportion of TCRαβ+ lymphocytes in MLNL	1307:1359	In addition, hesperidin increased the relative proportion of TCRαβ+ lymphocytes in MLNL.
30717392	10	45	from	decrease	1198:1205	arg1	concentration					1258:1270	monocyte chemotactic protein-1 concentration	1227:1270	monocyte chemotactic protein-1 concentration	1227:1270	These changes in the small intestine were accompanied by a decrease in interferon-γ and monocyte chemotactic protein-1 concentration.
30717392	10	45	from	decrease	1198:1205	arg1	interferon-γ					1210:1221	interferon-γ	1210:1221	interferon-γ	1210:1221	These changes in the small intestine were accompanied by a decrease in interferon-γ and monocyte chemotactic protein-1 concentration.
30717392	0	46	theme	Lymphoid	56:63	arg1	Tissue					65:70	Gut-Associated Lymphoid Tissue	41:70	Gut-Associated Lymphoid Tissue	41:70	Hesperidin Effects on Gut Microbiota and Gut-Associated Lymphoid Tissue in Healthy Rats.
30717392	8	47	theme	higher	938:943	arg1	number					945:950	a higher number	936:950	a higher number of bacteria and IgA-coated bacteria	936:986	Hesperidin administration resulted in a higher number of bacteria and IgA-coated bacteria, with changes in microbiota composition such as higher Lactobacillus proportion.
30717392	0	48	from	Tissue	65:70	arg1	Rats					83:86	Healthy Rats	75:86	Healthy Rats	75:86	Hesperidin Effects on Gut Microbiota and Gut-Associated Lymphoid Tissue in Healthy Rats.
30717392	2	49	theme	in-depth	194:201	arg1	investigation					203:215	a more in-depth investigation	187:215	a more in-depth investigation	187:215	Nonetheless, a more in-depth investigation is required on the effects on the immune system, and in particular, on the gut-associated lymphoid tissue, together with its relationship with the gut microbiota.
30717392	3	50	theme	hesperidin	435:444	arg1	administration					446:459	oral hesperidin administration	430:459	oral hesperidin administration	430:459	Therefore, we aimed to establish the influence of oral hesperidin administration on the intestinal lymphoid tissue and on the gut microbiota composition in healthy animals.
30717392	0	51	theme	Gut-Associated	41:54	arg1	Tissue					65:70	Gut-Associated Lymphoid Tissue	41:70	Gut-Associated Lymphoid Tissue	41:70	Hesperidin Effects on Gut Microbiota and Gut-Associated Lymphoid Tissue in Healthy Rats.
30717392	1	52	located	found	101:105	arg1	fruits					117:122	citrus fruits	110:122	citrus fruits	110:122	Hesperidin, found in citrus fruits, has shown a wide range of biological properties.
30717392	1	52	located	found	101:105	arg2	Hesperidin					89:98	Hesperidin	89:98	Hesperidin	89:98	Hesperidin, found in citrus fruits, has shown a wide range of biological properties.
30717392	8	53	theme	Hesperidin	898:907	arg1	administration					909:922	Hesperidin administration	898:922	Hesperidin administration	898:922	Hesperidin administration resulted in a higher number of bacteria and IgA-coated bacteria, with changes in microbiota composition such as higher Lactobacillus proportion.
30717392	1	54	theme	citrus	110:115	arg1	fruits					117:122	citrus fruits	110:122	citrus fruits	110:122	Hesperidin, found in citrus fruits, has shown a wide range of biological properties.
30717392	7	55	theme	small	865:869	arg1	intestine					871:879	the small intestine	861:879	the small intestine	861:879	IgA, cytokines, and gene expression in the small intestine were quantified.
30717392	8	56	theme	bacteria	979:986	arg1	number					945:950	a higher number	936:950	a higher number of bacteria and IgA-coated bacteria	936:986	Hesperidin administration resulted in a higher number of bacteria and IgA-coated bacteria, with changes in microbiota composition such as higher Lactobacillus proportion.
30717392	8	57	theme	IgA-coated	968:977	arg1	bacteria					979:986	IgA-coated bacteria	968:986	IgA-coated bacteria	968:986	Hesperidin administration resulted in a higher number of bacteria and IgA-coated bacteria, with changes in microbiota composition such as higher Lactobacillus proportion.
30717392	3	58	from	influence	417:425	arg1	tissue					488:493	the intestinal lymphoid tissue	464:493	the intestinal lymphoid tissue	464:493	Therefore, we aimed to establish the influence of oral hesperidin administration on the intestinal lymphoid tissue and on the gut microbiota composition in healthy animals.
30717392	3	58	from	influence	417:425	arg1	composition					521:531	the gut microbiota composition	502:531	the gut microbiota composition in healthy animals	502:550	Therefore, we aimed to establish the influence of oral hesperidin administration on the intestinal lymphoid tissue and on the gut microbiota composition in healthy animals.
30717392	10	59	theme	small	1160:1164	arg1	intestine					1166:1174	the small intestine	1156:1174	the small intestine	1156:1174	These changes in the small intestine were accompanied by a decrease in interferon-γ and monocyte chemotactic protein-1 concentration.
30717392	9	60	theme	intestine	1116:1124	arg1	content					1130:1136	the small intestine IgA content	1106:1136	the small intestine IgA content	1106:1136	Hesperidin was also able to increase the small intestine IgA content.
30717392	7	61	from	IgA	822:824	arg1	intestine					871:879	the small intestine	861:879	the small intestine	861:879	IgA, cytokines, and gene expression in the small intestine were quantified.
30717392	9	62	theme	IgA	1126:1128	arg1	content					1130:1136	the small intestine IgA content	1106:1136	the small intestine IgA content	1106:1136	Hesperidin was also able to increase the small intestine IgA content.
30717392	3	63	theme	oral	430:433	arg1	administration					446:459	oral hesperidin administration	430:459	oral hesperidin administration	430:459	Therefore, we aimed to establish the influence of oral hesperidin administration on the intestinal lymphoid tissue and on the gut microbiota composition in healthy animals.
30717392	3	64	from	composition	521:531	arg1	animals					544:550	healthy animals	536:550	healthy animals	536:550	Therefore, we aimed to establish the influence of oral hesperidin administration on the intestinal lymphoid tissue and on the gut microbiota composition in healthy animals.
30717392	10	65	theme	protein-1	1248:1256	arg1	concentration					1258:1270	monocyte chemotactic protein-1 concentration	1227:1270	monocyte chemotactic protein-1 concentration	1227:1270	These changes in the small intestine were accompanied by a decrease in interferon-γ and monocyte chemotactic protein-1 concentration.
30717392	3	66	theme	intestinal	468:477	arg1	tissue					488:493	the intestinal lymphoid tissue	464:493	the intestinal lymphoid tissue	464:493	Therefore, we aimed to establish the influence of oral hesperidin administration on the intestinal lymphoid tissue and on the gut microbiota composition in healthy animals.
30717392	5	67	theme	caecal	721:726	arg1	content					728:734	caecal content	721:734	caecal content	721:734	Microbiota composition and IgA-coated bacteria were determined in caecal content.
30717392	7	68	from	cytokines	827:835	arg1	intestine					871:879	the small intestine	861:879	the small intestine	861:879	IgA, cytokines, and gene expression in the small intestine were quantified.
30717392	3	69	theme	lymphoid	479:486	arg1	tissue					488:493	the intestinal lymphoid tissue	464:493	the intestinal lymphoid tissue	464:493	Therefore, we aimed to establish the influence of oral hesperidin administration on the intestinal lymphoid tissue and on the gut microbiota composition in healthy animals.
30717392	7	70	theme	gene	842:845	arg1	expression					847:856	gene expression	842:856	gene expression in the small intestine	842:879	IgA, cytokines, and gene expression in the small intestine were quantified.
30473696	9	0	theme	infection	1879:1887	arg1	interaction					1846:1856	an interaction	1843:1856	an interaction of diet and helminth infection that stimulates the growth of beneficial bacterial species	1843:1946	Interestingly, pigs treated with both inulin and T. suis displayed the highest Bacteroidetes: Firmicutes ratio and the lowest gut pH, suggesting an interaction of diet and helminth infection that stimulates the growth of beneficial bacterial species.
30473696	10	1	from	changes	2105:2111	arg1	composition					2127:2137	microbiota composition	2116:2137	microbiota composition	2116:2137	Overall, our data demonstrate that T. suis infection and inulin co-operatively enhance anti-inflammatory immune responses, which is potentially mediated by changes in microbiota composition.
30473696	3	2	theme	T-helper	550:557	arg1	Th					570:571	T-helper cell type (Th) 2 and T-regulatory immune responses	550:608	Th	570:571	It has been shown that colonization with gastrointestinal parasitic worms (helminths) may alleviate chronic inflammation through promotion of T-helper cell type (Th) 2 and T-regulatory immune responses and alterations in the gut microbiome.
30473696	11	3	theme	intricate	2166:2174	arg1	interactions					2176:2187	the intricate interactions	2162:2187	the intricate interactions between diet, immune function and microbiota composition	2162:2244	Our results highlight the intricate interactions between diet, immune function and microbiota composition in a porcine helminth infection model.
30473696	5	4	theme	immune	854:859	arg1	response					861:868	a typical Th2-biased immune response	833:868	a typical Th2-biased immune response characterized by transcriptional changes in Th2- and barrier function-related genes	833:952	T. suis infection induced a typical Th2-biased immune response characterized by transcriptional changes in Th2- and barrier function-related genes, accompanied by intestinal remodeling through increased epithelial goblet and tuft cell proliferation.
30473696	9	5	theme	beneficial	1919:1928	arg1	species					1940:1946	beneficial bacterial species	1919:1946	beneficial bacterial species	1919:1946	Interestingly, pigs treated with both inulin and T. suis displayed the highest Bacteroidetes: Firmicutes ratio and the lowest gut pH, suggesting an interaction of diet and helminth infection that stimulates the growth of beneficial bacterial species.
30473696	3	6	theme	Th	570:571	arg1	promotion					537:545	promotion	537:545	promotion of T-helper cell type (Th) 2 and T-regulatory immune responses	537:608	It has been shown that colonization with gastrointestinal parasitic worms (helminths) may alleviate chronic inflammation through promotion of T-helper cell type (Th) 2 and T-regulatory immune responses and alterations in the gut microbiome.
30473696	3	6	theme	Th	570:571	arg1	alterations					614:624	alterations	614:624	alterations in the gut microbiome	614:646	It has been shown that colonization with gastrointestinal parasitic worms (helminths) may alleviate chronic inflammation through promotion of T-helper cell type (Th) 2 and T-regulatory immune responses and alterations in the gut microbiome.
30473696	1	7	theme	host	162:165	arg1	responses					174:182	shaping host immune responses	154:182	shaping host immune responses	154:182	Diet composition may play a crucial role in shaping host immune responses and commensal gut microbiota populations.
30473696	6	8	theme	pro-inflammatory	1164:1179	arg1	genes					1181:1185	suppressed Th1-related pro-inflammatory genes	1141:1185	suppressed Th1-related pro-inflammatory genes (IFNG, IL1A, IL8)	1141:1203	We observed that inulin also up-regulated Th2-related immune genes (IL13, IL5), and suppressed Th1-related pro-inflammatory genes (IFNG, IL1A, IL8) in the colon.
30473696	9	9	theme	species	1940:1946	arg1	growth					1909:1914	the growth	1905:1914	the growth of beneficial bacterial species	1905:1946	Interestingly, pigs treated with both inulin and T. suis displayed the highest Bacteroidetes: Firmicutes ratio and the lowest gut pH, suggesting an interaction of diet and helminth infection that stimulates the growth of beneficial bacterial species.
30473696	1	10	theme	commensal	188:196	arg1	populations					213:223	commensal gut microbiota populations	188:223	commensal gut microbiota populations	188:223	Diet composition may play a crucial role in shaping host immune responses and commensal gut microbiota populations.
30473696	3	11	theme	type	564:567	arg1	Th					570:571	T-helper cell type (Th) 2 and T-regulatory immune responses	550:608	Th	570:571	It has been shown that colonization with gastrointestinal parasitic worms (helminths) may alleviate chronic inflammation through promotion of T-helper cell type (Th) 2 and T-regulatory immune responses and alterations in the gut microbiome.
30473696	3	12	theme	T-regulatory	580:591	arg1	responses					600:608	T-helper cell type (Th) 2 and T-regulatory immune responses	550:608	responses	600:608	It has been shown that colonization with gastrointestinal parasitic worms (helminths) may alleviate chronic inflammation through promotion of T-helper cell type (Th) 2 and T-regulatory immune responses and alterations in the gut microbiome.
30473696	7	13	dep	IL13	1348:1351	arg1	e.g.					1342:1345	e.g.	1342:1345	e.g.	1342:1345	Notably, inulin augmented the T. suis-induced responses with increased transcription of key Th2 and mucosal barrier genes (e.g., IL13, TFF3), and synergistically suppressed pro-inflammatory genes, such as IFNG and CXCL9.
30473696	10	14	theme	T.	1984:1985	arg1	infection					1992:2000	T. suis infection	1984:2000	T. suis infection	1984:2000	Overall, our data demonstrate that T. suis infection and inulin co-operatively enhance anti-inflammatory immune responses, which is potentially mediated by changes in microbiota composition.
30473696	8	15	theme	16S	1440:1442	arg1	sequencing					1449:1458	16S rRNA sequencing	1440:1458	16S rRNA sequencing of proximal colon digesta samples	1440:1492	16S rRNA sequencing of proximal colon digesta samples revealed that inulin supplementation reduced the abundance of bacterial phyla linked to inflammation, such as Proteobacteria and Firmicutes, and simultaneously increased Actinobacteria and Bacteroidetes.
30473696	5	16	theme	function-related	931:946	arg1	genes					948:952	barrier function-related genes	923:952	barrier function-related genes	923:952	T. suis infection induced a typical Th2-biased immune response characterized by transcriptional changes in Th2- and barrier function-related genes, accompanied by intestinal remodeling through increased epithelial goblet and tuft cell proliferation.
30473696	5	17	theme	barrier	923:929	arg1	genes					948:952	barrier function-related genes	923:952	barrier function-related genes	923:952	T. suis infection induced a typical Th2-biased immune response characterized by transcriptional changes in Th2- and barrier function-related genes, accompanied by intestinal remodeling through increased epithelial goblet and tuft cell proliferation.
30473696	11	18	theme	porcine	2251:2257	arg1	model					2278:2282	a porcine helminth infection model	2249:2282	a porcine helminth infection model	2249:2282	Our results highlight the intricate interactions between diet, immune function and microbiota composition in a porcine helminth infection model.
30473696	12	19	theme	bioactive	2361:2369	arg1	diets					2371:2375	bioactive diets	2361:2375	bioactive diets	2361:2375	This porcine model should facilitate further investigations into the use of bioactive diets as immunomodulatory mediators against inflammatory conditions, and how diet and parasites may influence gut health.
30473696	3	20	theme	responses	600:608	arg1	promotion					537:545	promotion	537:545	promotion of T-helper cell type (Th) 2 and T-regulatory immune responses	537:608	It has been shown that colonization with gastrointestinal parasitic worms (helminths) may alleviate chronic inflammation through promotion of T-helper cell type (Th) 2 and T-regulatory immune responses and alterations in the gut microbiome.
30473696	3	20	theme	responses	600:608	arg1	alterations					614:624	alterations	614:624	alterations in the gut microbiome	614:646	It has been shown that colonization with gastrointestinal parasitic worms (helminths) may alleviate chronic inflammation through promotion of T-helper cell type (Th) 2 and T-regulatory immune responses and alterations in the gut microbiome.
30473696	8	21	attach	linked	1572:1577	arg2	phyla					1566:1570	bacterial phyla	1556:1570	bacterial phyla	1556:1570	16S rRNA sequencing of proximal colon digesta samples revealed that inulin supplementation reduced the abundance of bacterial phyla linked to inflammation, such as Proteobacteria and Firmicutes, and simultaneously increased Actinobacteria and Bacteroidetes.
30473696	8	21	attach	linked	1572:1577	arg2	Firmicutes					1623:1632	Firmicutes	1623:1632	Firmicutes	1623:1632	16S rRNA sequencing of proximal colon digesta samples revealed that inulin supplementation reduced the abundance of bacterial phyla linked to inflammation, such as Proteobacteria and Firmicutes, and simultaneously increased Actinobacteria and Bacteroidetes.
30473696	8	21	attach	linked	1572:1577	arg1	inflammation					1582:1593	inflammation	1582:1593	inflammation	1582:1593	16S rRNA sequencing of proximal colon digesta samples revealed that inulin supplementation reduced the abundance of bacterial phyla linked to inflammation, such as Proteobacteria and Firmicutes, and simultaneously increased Actinobacteria and Bacteroidetes.
30473696	8	21	attach	linked	1572:1577	arg2	Proteobacteria					1604:1617	Proteobacteria	1604:1617	Proteobacteria	1604:1617	16S rRNA sequencing of proximal colon digesta samples revealed that inulin supplementation reduced the abundance of bacterial phyla linked to inflammation, such as Proteobacteria and Firmicutes, and simultaneously increased Actinobacteria and Bacteroidetes.
30473696	5	22	theme	transcriptional	887:901	arg1	changes					903:909	transcriptional changes	887:909	transcriptional changes in Th2- and barrier function-related genes	887:952	T. suis infection induced a typical Th2-biased immune response characterized by transcriptional changes in Th2- and barrier function-related genes, accompanied by intestinal remodeling through increased epithelial goblet and tuft cell proliferation.
30473696	8	23	theme	proximal	1463:1470	arg1	samples					1486:1492	proximal colon digesta samples	1463:1492	proximal colon digesta samples	1463:1492	16S rRNA sequencing of proximal colon digesta samples revealed that inulin supplementation reduced the abundance of bacterial phyla linked to inflammation, such as Proteobacteria and Firmicutes, and simultaneously increased Actinobacteria and Bacteroidetes.
30473696	3	24	from	promotion	537:545	arg1	microbiome					637:646	the gut microbiome	629:646	the gut microbiome	629:646	It has been shown that colonization with gastrointestinal parasitic worms (helminths) may alleviate chronic inflammation through promotion of T-helper cell type (Th) 2 and T-regulatory immune responses and alterations in the gut microbiome.
30473696	5	25	from	changes	903:909	arg1	genes					948:952	barrier function-related genes	923:952	barrier function-related genes	923:952	T. suis infection induced a typical Th2-biased immune response characterized by transcriptional changes in Th2- and barrier function-related genes, accompanied by intestinal remodeling through increased epithelial goblet and tuft cell proliferation.
30473696	5	25	from	changes	903:909	arg1	Th2-					914:917	Th2-	914:917	Th2-	914:917	T. suis infection induced a typical Th2-biased immune response characterized by transcriptional changes in Th2- and barrier function-related genes, accompanied by intestinal remodeling through increased epithelial goblet and tuft cell proliferation.
30473696	1	26	theme	crucial	138:144	arg1	role					146:149	a crucial role	136:149	a crucial role	136:149	Diet composition may play a crucial role in shaping host immune responses and commensal gut microbiota populations.
30473696	8	27	theme	digesta	1478:1484	arg1	samples					1486:1492	proximal colon digesta samples	1463:1492	proximal colon digesta samples	1463:1492	16S rRNA sequencing of proximal colon digesta samples revealed that inulin supplementation reduced the abundance of bacterial phyla linked to inflammation, such as Proteobacteria and Firmicutes, and simultaneously increased Actinobacteria and Bacteroidetes.
30473696	10	28	theme	immune	2054:2059	arg1	responses					2061:2069	anti-inflammatory immune responses	2036:2069	anti-inflammatory immune responses	2036:2069	Overall, our data demonstrate that T. suis infection and inulin co-operatively enhance anti-inflammatory immune responses, which is potentially mediated by changes in microbiota composition.
30473696	9	29	theme	Firmicutes	1792:1801	arg1	Bacteroidetes					1777:1789	the highest Bacteroidetes	1765:1789	the highest Bacteroidetes: Firmicutes ratio and the lowest gut pH	1765:1829	Interestingly, pigs treated with both inulin and T. suis displayed the highest Bacteroidetes: Firmicutes ratio and the lowest gut pH, suggesting an interaction of diet and helminth infection that stimulates the growth of beneficial bacterial species.
30473696	9	29	theme	Firmicutes	1792:1801	arg1	ratio					1803:1807	Firmicutes ratio	1792:1807	Firmicutes ratio	1792:1807	Interestingly, pigs treated with both inulin and T. suis displayed the highest Bacteroidetes: Firmicutes ratio and the lowest gut pH, suggesting an interaction of diet and helminth infection that stimulates the growth of beneficial bacterial species.
30473696	12	30	theme	porcine	2290:2296	arg1	model					2298:2302	This porcine model	2285:2302	This porcine model	2285:2302	This porcine model should facilitate further investigations into the use of bioactive diets as immunomodulatory mediators against inflammatory conditions, and how diet and parasites may influence gut health.
30473696	5	31	theme	increased	1000:1008	arg1	goblet					1021:1026	increased epithelial goblet and tuft cell proliferation	1000:1054	goblet	1021:1026	T. suis infection induced a typical Th2-biased immune response characterized by transcriptional changes in Th2- and barrier function-related genes, accompanied by intestinal remodeling through increased epithelial goblet and tuft cell proliferation.
30473696	7	32	theme	genes	1335:1339	arg1	transcription					1290:1302	increased transcription	1280:1302	increased transcription of key Th2 and mucosal barrier genes (e.g., IL13, TFF3)	1280:1358	Notably, inulin augmented the T. suis-induced responses with increased transcription of key Th2 and mucosal barrier genes (e.g., IL13, TFF3), and synergistically suppressed pro-inflammatory genes, such as IFNG and CXCL9.
30473696	8	33	theme	inulin	1508:1513	arg1	supplementation					1515:1529	inulin supplementation	1508:1529	inulin supplementation	1508:1529	16S rRNA sequencing of proximal colon digesta samples revealed that inulin supplementation reduced the abundance of bacterial phyla linked to inflammation, such as Proteobacteria and Firmicutes, and simultaneously increased Actinobacteria and Bacteroidetes.
30473696	4	34	theme	immune	721:726	arg1	function					728:735	mucosal immune function	713:735	mucosal immune function in pigs	713:743	In this study, we investigated if dietary inulin could modulate mucosal immune function in pigs during colonization with the porcine whipworm Trichuris suis.
30473696	3	35	from	alterations	614:624	arg1	microbiome					637:646	the gut microbiome	629:646	the gut microbiome	629:646	It has been shown that colonization with gastrointestinal parasitic worms (helminths) may alleviate chronic inflammation through promotion of T-helper cell type (Th) 2 and T-regulatory immune responses and alterations in the gut microbiome.
30473696	2	36	theme	immune	365:370	arg1	function					372:379	gut immune function	361:379	gut immune function	361:379	Bioactive dietary components, such as inulin, have been extensively studied for their bioactive properties, particularly in modulating gut immune function and reducing inflammation.
30473696	0	37	theme	Mucosal	0:6	arg1	Barrier					8:14	Mucosal Barrier	0:14	Mucosal Barrier	0:14	Mucosal Barrier and Th2 Immune Responses Are Enhanced by Dietary Inulin in Pigs Infected With Trichuris suis.
30473696	12	38	dep	facilitate	2311:2320	arg1	influence					2471:2479	influence	2471:2479	may influence gut health	2467:2490	This porcine model should facilitate further investigations into the use of bioactive diets as immunomodulatory mediators against inflammatory conditions, and how diet and parasites may influence gut health.
30473696	7	39	theme	mucosal	1319:1325	arg1	barrier					1327:1333	mucosal barrier	1319:1333	mucosal barrier	1319:1333	Notably, inulin augmented the T. suis-induced responses with increased transcription of key Th2 and mucosal barrier genes (e.g., IL13, TFF3), and synergistically suppressed pro-inflammatory genes, such as IFNG and CXCL9.
30473696	9	40	theme	lowest	1817:1822	arg1	Bacteroidetes					1777:1789	the highest Bacteroidetes	1765:1789	the highest Bacteroidetes: Firmicutes ratio and the lowest gut pH	1765:1829	Interestingly, pigs treated with both inulin and T. suis displayed the highest Bacteroidetes: Firmicutes ratio and the lowest gut pH, suggesting an interaction of diet and helminth infection that stimulates the growth of beneficial bacterial species.
30473696	9	40	theme	lowest	1817:1822	arg1	pH					1828:1829	the lowest gut pH	1813:1829	the lowest gut pH	1813:1829	Interestingly, pigs treated with both inulin and T. suis displayed the highest Bacteroidetes: Firmicutes ratio and the lowest gut pH, suggesting an interaction of diet and helminth infection that stimulates the growth of beneficial bacterial species.
30473696	2	41	theme	dietary	236:242	arg1	inulin					264:269	inulin	264:269	inulin	264:269	Bioactive dietary components, such as inulin, have been extensively studied for their bioactive properties, particularly in modulating gut immune function and reducing inflammation.
30473696	2	41	theme	dietary	236:242	arg1	components					244:253	Bioactive dietary components	226:253	Bioactive dietary components	226:253	Bioactive dietary components, such as inulin, have been extensively studied for their bioactive properties, particularly in modulating gut immune function and reducing inflammation.
30473696	6	42	dep	genes	1118:1122	arg1	IL5					1131:1133	IL5	1131:1133	IL5	1131:1133	We observed that inulin also up-regulated Th2-related immune genes (IL13, IL5), and suppressed Th1-related pro-inflammatory genes (IFNG, IL1A, IL8) in the colon.
30473696	6	42	dep	genes	1118:1122	arg1	IL13					1125:1128	IL13	1125:1128	IL13	1125:1128	We observed that inulin also up-regulated Th2-related immune genes (IL13, IL5), and suppressed Th1-related pro-inflammatory genes (IFNG, IL1A, IL8) in the colon.
30473696	3	43	theme	gastrointestinal	449:464	arg1	helminths					483:491	helminths	483:491	helminths	483:491	It has been shown that colonization with gastrointestinal parasitic worms (helminths) may alleviate chronic inflammation through promotion of T-helper cell type (Th) 2 and T-regulatory immune responses and alterations in the gut microbiome.
30473696	3	43	theme	gastrointestinal	449:464	arg1	worms					476:480	gastrointestinal parasitic worms	449:480	gastrointestinal parasitic worms (helminths)	449:492	It has been shown that colonization with gastrointestinal parasitic worms (helminths) may alleviate chronic inflammation through promotion of T-helper cell type (Th) 2 and T-regulatory immune responses and alterations in the gut microbiome.
30473696	5	44	theme	suis	810:813	arg1	infection					815:823	T. suis infection	807:823	T. suis infection	807:823	T. suis infection induced a typical Th2-biased immune response characterized by transcriptional changes in Th2- and barrier function-related genes, accompanied by intestinal remodeling through increased epithelial goblet and tuft cell proliferation.
30473696	7	45	theme	Th2	1311:1313	arg1	genes					1335:1339	key Th2 and mucosal barrier genes	1307:1339	key Th2 and mucosal barrier genes (e.g., IL13, TFF3)	1307:1358	Notably, inulin augmented the T. suis-induced responses with increased transcription of key Th2 and mucosal barrier genes (e.g., IL13, TFF3), and synergistically suppressed pro-inflammatory genes, such as IFNG and CXCL9.
30473696	11	46	theme	microbiota	2223:2232	arg1	composition					2234:2244	microbiota composition	2223:2244	microbiota composition	2223:2244	Our results highlight the intricate interactions between diet, immune function and microbiota composition in a porcine helminth infection model.
30473696	0	47	theme	Immune	24:29	arg1	Responses					31:39	Th2 Immune Responses	20:39	Th2 Immune Responses	20:39	Mucosal Barrier and Th2 Immune Responses Are Enhanced by Dietary Inulin in Pigs Infected With Trichuris suis.
30473696	8	48	theme	bacterial	1556:1564	arg1	phyla					1566:1570	bacterial phyla	1556:1570	bacterial phyla	1556:1570	16S rRNA sequencing of proximal colon digesta samples revealed that inulin supplementation reduced the abundance of bacterial phyla linked to inflammation, such as Proteobacteria and Firmicutes, and simultaneously increased Actinobacteria and Bacteroidetes.
30473696	8	48	theme	bacterial	1556:1564	arg1	Firmicutes					1623:1632	Firmicutes	1623:1632	Firmicutes	1623:1632	16S rRNA sequencing of proximal colon digesta samples revealed that inulin supplementation reduced the abundance of bacterial phyla linked to inflammation, such as Proteobacteria and Firmicutes, and simultaneously increased Actinobacteria and Bacteroidetes.
30473696	8	48	theme	bacterial	1556:1564	arg1	Proteobacteria					1604:1617	Proteobacteria	1604:1617	Proteobacteria	1604:1617	16S rRNA sequencing of proximal colon digesta samples revealed that inulin supplementation reduced the abundance of bacterial phyla linked to inflammation, such as Proteobacteria and Firmicutes, and simultaneously increased Actinobacteria and Bacteroidetes.
30473696	7	49	dep	genes	1335:1339	arg1	TFF3					1354:1357	TFF3	1354:1357	TFF3	1354:1357	Notably, inulin augmented the T. suis-induced responses with increased transcription of key Th2 and mucosal barrier genes (e.g., IL13, TFF3), and synergistically suppressed pro-inflammatory genes, such as IFNG and CXCL9.
30473696	7	49	dep	genes	1335:1339	arg1	IL13					1348:1351	IL13	1348:1351	IL13	1348:1351	Notably, inulin augmented the T. suis-induced responses with increased transcription of key Th2 and mucosal barrier genes (e.g., IL13, TFF3), and synergistically suppressed pro-inflammatory genes, such as IFNG and CXCL9.
30473696	7	50	theme	increased	1280:1288	arg1	transcription					1290:1302	increased transcription	1280:1302	increased transcription of key Th2 and mucosal barrier genes (e.g., IL13, TFF3)	1280:1358	Notably, inulin augmented the T. suis-induced responses with increased transcription of key Th2 and mucosal barrier genes (e.g., IL13, TFF3), and synergistically suppressed pro-inflammatory genes, such as IFNG and CXCL9.
30473696	2	51	theme	Bioactive	226:234	arg1	inulin					264:269	inulin	264:269	inulin	264:269	Bioactive dietary components, such as inulin, have been extensively studied for their bioactive properties, particularly in modulating gut immune function and reducing inflammation.
30473696	2	51	theme	Bioactive	226:234	arg1	components					244:253	Bioactive dietary components	226:253	Bioactive dietary components	226:253	Bioactive dietary components, such as inulin, have been extensively studied for their bioactive properties, particularly in modulating gut immune function and reducing inflammation.
30473696	4	52	theme	porcine	774:780	arg1	suis					801:804	the porcine whipworm Trichuris suis	770:804	the porcine whipworm Trichuris suis	770:804	In this study, we investigated if dietary inulin could modulate mucosal immune function in pigs during colonization with the porcine whipworm Trichuris suis.
30473696	1	53	theme	gut	198:200	arg1	populations					213:223	commensal gut microbiota populations	188:223	commensal gut microbiota populations	188:223	Diet composition may play a crucial role in shaping host immune responses and commensal gut microbiota populations.
30473696	9	54	theme	diet	1861:1864	arg1	interaction					1846:1856	an interaction	1843:1856	an interaction of diet and helminth infection that stimulates the growth of beneficial bacterial species	1843:1946	Interestingly, pigs treated with both inulin and T. suis displayed the highest Bacteroidetes: Firmicutes ratio and the lowest gut pH, suggesting an interaction of diet and helminth infection that stimulates the growth of beneficial bacterial species.
30473696	4	55	theme	Trichuris	791:799	arg1	suis					801:804	the porcine whipworm Trichuris suis	770:804	the porcine whipworm Trichuris suis	770:804	In this study, we investigated if dietary inulin could modulate mucosal immune function in pigs during colonization with the porcine whipworm Trichuris suis.
30473696	3	56	theme	chronic	508:514	arg1	inflammation					516:527	chronic inflammation	508:527	chronic inflammation	508:527	It has been shown that colonization with gastrointestinal parasitic worms (helminths) may alleviate chronic inflammation through promotion of T-helper cell type (Th) 2 and T-regulatory immune responses and alterations in the gut microbiome.
30473696	5	57	theme	tuft	1032:1035	arg1	proliferation					1042:1054	increased epithelial goblet and tuft cell proliferation	1000:1054	proliferation	1042:1054	T. suis infection induced a typical Th2-biased immune response characterized by transcriptional changes in Th2- and barrier function-related genes, accompanied by intestinal remodeling through increased epithelial goblet and tuft cell proliferation.
30473696	7	58	theme	T.	1249:1250	arg1	responses					1265:1273	the T. suis-induced responses	1245:1273	the T. suis-induced responses	1245:1273	Notably, inulin augmented the T. suis-induced responses with increased transcription of key Th2 and mucosal barrier genes (e.g., IL13, TFF3), and synergistically suppressed pro-inflammatory genes, such as IFNG and CXCL9.
30473696	9	59	theme	helminth	1870:1877	arg1	infection					1879:1887	helminth infection	1870:1887	helminth infection	1870:1887	Interestingly, pigs treated with both inulin and T. suis displayed the highest Bacteroidetes: Firmicutes ratio and the lowest gut pH, suggesting an interaction of diet and helminth infection that stimulates the growth of beneficial bacterial species.
30473696	12	60	theme	further	2322:2328	arg1	investigations					2330:2343	further investigations	2322:2343	further investigations into the use of bioactive diets	2322:2375	This porcine model should facilitate further investigations into the use of bioactive diets as immunomodulatory mediators against inflammatory conditions, and how diet and parasites may influence gut health.
30473696	12	60	theme	further	2322:2328	arg1	mediators					2397:2405	immunomodulatory mediators	2380:2405	immunomodulatory mediators against inflammatory conditions	2380:2437	This porcine model should facilitate further investigations into the use of bioactive diets as immunomodulatory mediators against inflammatory conditions, and how diet and parasites may influence gut health.
30473696	12	61	theme	inflammatory	2415:2426	arg1	conditions					2428:2437	inflammatory conditions	2415:2437	inflammatory conditions	2415:2437	This porcine model should facilitate further investigations into the use of bioactive diets as immunomodulatory mediators against inflammatory conditions, and how diet and parasites may influence gut health.
30473696	5	62	theme	Th2-biased	843:852	arg1	response					861:868	a typical Th2-biased immune response	833:868	a typical Th2-biased immune response characterized by transcriptional changes in Th2- and barrier function-related genes	833:952	T. suis infection induced a typical Th2-biased immune response characterized by transcriptional changes in Th2- and barrier function-related genes, accompanied by intestinal remodeling through increased epithelial goblet and tuft cell proliferation.
30473696	1	63	theme	shaping	154:160	arg1	responses					174:182	shaping host immune responses	154:182	shaping host immune responses	154:182	Diet composition may play a crucial role in shaping host immune responses and commensal gut microbiota populations.
30473696	7	64	theme	pro-inflammatory	1392:1407	arg1	CXCL9					1433:1437	CXCL9	1433:1437	CXCL9	1433:1437	Notably, inulin augmented the T. suis-induced responses with increased transcription of key Th2 and mucosal barrier genes (e.g., IL13, TFF3), and synergistically suppressed pro-inflammatory genes, such as IFNG and CXCL9.
30473696	7	64	theme	pro-inflammatory	1392:1407	arg1	IFNG					1424:1427	IFNG	1424:1427	IFNG	1424:1427	Notably, inulin augmented the T. suis-induced responses with increased transcription of key Th2 and mucosal barrier genes (e.g., IL13, TFF3), and synergistically suppressed pro-inflammatory genes, such as IFNG and CXCL9.
30473696	7	64	theme	pro-inflammatory	1392:1407	arg1	genes					1409:1413	pro-inflammatory genes	1392:1413	pro-inflammatory genes	1392:1413	Notably, inulin augmented the T. suis-induced responses with increased transcription of key Th2 and mucosal barrier genes (e.g., IL13, TFF3), and synergistically suppressed pro-inflammatory genes, such as IFNG and CXCL9.
30473696	9	65	theme	bacterial	1930:1938	arg1	species					1940:1946	beneficial bacterial species	1919:1946	beneficial bacterial species	1919:1946	Interestingly, pigs treated with both inulin and T. suis displayed the highest Bacteroidetes: Firmicutes ratio and the lowest gut pH, suggesting an interaction of diet and helminth infection that stimulates the growth of beneficial bacterial species.
30473696	6	66	dep	genes	1181:1185	arg1	IFNG					1188:1191	IFNG	1188:1191	IFNG	1188:1191	We observed that inulin also up-regulated Th2-related immune genes (IL13, IL5), and suppressed Th1-related pro-inflammatory genes (IFNG, IL1A, IL8) in the colon.
30473696	4	67	with	colonization	752:763	arg1	suis					801:804	the porcine whipworm Trichuris suis	770:804	the porcine whipworm Trichuris suis	770:804	In this study, we investigated if dietary inulin could modulate mucosal immune function in pigs during colonization with the porcine whipworm Trichuris suis.
30473696	3	68	with	colonization	431:442	arg1	helminths					483:491	helminths	483:491	helminths	483:491	It has been shown that colonization with gastrointestinal parasitic worms (helminths) may alleviate chronic inflammation through promotion of T-helper cell type (Th) 2 and T-regulatory immune responses and alterations in the gut microbiome.
30473696	3	68	with	colonization	431:442	arg1	worms					476:480	gastrointestinal parasitic worms	449:480	gastrointestinal parasitic worms (helminths)	449:492	It has been shown that colonization with gastrointestinal parasitic worms (helminths) may alleviate chronic inflammation through promotion of T-helper cell type (Th) 2 and T-regulatory immune responses and alterations in the gut microbiome.
30473696	3	69	theme	cell	559:562	arg1	Th					570:571	T-helper cell type (Th) 2 and T-regulatory immune responses	550:608	Th	570:571	It has been shown that colonization with gastrointestinal parasitic worms (helminths) may alleviate chronic inflammation through promotion of T-helper cell type (Th) 2 and T-regulatory immune responses and alterations in the gut microbiome.
30473696	1	70	theme	immune	167:172	arg1	responses					174:182	shaping host immune responses	154:182	shaping host immune responses	154:182	Diet composition may play a crucial role in shaping host immune responses and commensal gut microbiota populations.
30473696	12	71	theme	immunomodulatory	2380:2395	arg1	investigations					2330:2343	further investigations	2322:2343	further investigations into the use of bioactive diets	2322:2375	This porcine model should facilitate further investigations into the use of bioactive diets as immunomodulatory mediators against inflammatory conditions, and how diet and parasites may influence gut health.
30473696	12	71	theme	immunomodulatory	2380:2395	arg1	mediators					2397:2405	immunomodulatory mediators	2380:2405	immunomodulatory mediators against inflammatory conditions	2380:2437	This porcine model should facilitate further investigations into the use of bioactive diets as immunomodulatory mediators against inflammatory conditions, and how diet and parasites may influence gut health.
30473696	6	72	theme	Th1-related	1152:1162	arg1	genes					1181:1185	suppressed Th1-related pro-inflammatory genes	1141:1185	suppressed Th1-related pro-inflammatory genes (IFNG, IL1A, IL8)	1141:1203	We observed that inulin also up-regulated Th2-related immune genes (IL13, IL5), and suppressed Th1-related pro-inflammatory genes (IFNG, IL1A, IL8) in the colon.
30473696	6	73	dep	IFNG	1188:1191	arg1	IL8					1200:1202	IL8	1200:1202	IL8	1200:1202	We observed that inulin also up-regulated Th2-related immune genes (IL13, IL5), and suppressed Th1-related pro-inflammatory genes (IFNG, IL1A, IL8) in the colon.
30473696	6	73	dep	IFNG	1188:1191	arg1	IL1A					1194:1197	IL1A	1194:1197	IL1A	1194:1197	We observed that inulin also up-regulated Th2-related immune genes (IL13, IL5), and suppressed Th1-related pro-inflammatory genes (IFNG, IL1A, IL8) in the colon.
30473696	12	74	theme	gut	2481:2483	arg1	health					2485:2490	gut health	2481:2490	gut health	2481:2490	This porcine model should facilitate further investigations into the use of bioactive diets as immunomodulatory mediators against inflammatory conditions, and how diet and parasites may influence gut health.
30473696	10	75	theme	suis	1987:1990	arg1	infection					1992:2000	T. suis infection	1984:2000	T. suis infection	1984:2000	Overall, our data demonstrate that T. suis infection and inulin co-operatively enhance anti-inflammatory immune responses, which is potentially mediated by changes in microbiota composition.
30473696	8	76	theme	rRNA	1444:1447	arg1	sequencing					1449:1458	16S rRNA sequencing	1440:1458	16S rRNA sequencing of proximal colon digesta samples	1440:1492	16S rRNA sequencing of proximal colon digesta samples revealed that inulin supplementation reduced the abundance of bacterial phyla linked to inflammation, such as Proteobacteria and Firmicutes, and simultaneously increased Actinobacteria and Bacteroidetes.
30473696	4	77	theme	dietary	683:689	arg1	inulin					691:696	dietary inulin	683:696	dietary inulin	683:696	In this study, we investigated if dietary inulin could modulate mucosal immune function in pigs during colonization with the porcine whipworm Trichuris suis.
30473696	9	78	dep	Bacteroidetes	1777:1789	arg1	Bacteroidetes					1777:1789	the highest Bacteroidetes	1765:1789	the highest Bacteroidetes: Firmicutes ratio and the lowest gut pH	1765:1829	Interestingly, pigs treated with both inulin and T. suis displayed the highest Bacteroidetes: Firmicutes ratio and the lowest gut pH, suggesting an interaction of diet and helminth infection that stimulates the growth of beneficial bacterial species.
30473696	9	78	dep	Bacteroidetes	1777:1789	arg1	pH					1828:1829	the lowest gut pH	1813:1829	the lowest gut pH	1813:1829	Interestingly, pigs treated with both inulin and T. suis displayed the highest Bacteroidetes: Firmicutes ratio and the lowest gut pH, suggesting an interaction of diet and helminth infection that stimulates the growth of beneficial bacterial species.
30473696	9	78	dep	Bacteroidetes	1777:1789	arg1	ratio					1803:1807	Firmicutes ratio	1792:1807	Firmicutes ratio	1792:1807	Interestingly, pigs treated with both inulin and T. suis displayed the highest Bacteroidetes: Firmicutes ratio and the lowest gut pH, suggesting an interaction of diet and helminth infection that stimulates the growth of beneficial bacterial species.
30473696	1	79	theme	Diet	110:113	arg1	composition					115:125	Diet composition	110:125	Diet composition	110:125	Diet composition may play a crucial role in shaping host immune responses and commensal gut microbiota populations.
30473696	12	80	theme	diets	2371:2375	arg1	use					2354:2356	the use	2350:2356	the use of bioactive diets	2350:2375	This porcine model should facilitate further investigations into the use of bioactive diets as immunomodulatory mediators against inflammatory conditions, and how diet and parasites may influence gut health.
30473696	0	81	theme	Dietary	57:63	arg1	Inulin					65:70	Dietary Inulin	57:70	Dietary Inulin	57:70	Mucosal Barrier and Th2 Immune Responses Are Enhanced by Dietary Inulin in Pigs Infected With Trichuris suis.
30473696	3	82	theme	immune	593:598	arg1	responses					600:608	T-helper cell type (Th) 2 and T-regulatory immune responses	550:608	responses	600:608	It has been shown that colonization with gastrointestinal parasitic worms (helminths) may alleviate chronic inflammation through promotion of T-helper cell type (Th) 2 and T-regulatory immune responses and alterations in the gut microbiome.
30473696	11	83	theme	helminth	2259:2266	arg1	model					2278:2282	a porcine helminth infection model	2249:2282	a porcine helminth infection model	2249:2282	Our results highlight the intricate interactions between diet, immune function and microbiota composition in a porcine helminth infection model.
30473696	8	84	theme	colon	1472:1476	arg1	samples					1486:1492	proximal colon digesta samples	1463:1492	proximal colon digesta samples	1463:1492	16S rRNA sequencing of proximal colon digesta samples revealed that inulin supplementation reduced the abundance of bacterial phyla linked to inflammation, such as Proteobacteria and Firmicutes, and simultaneously increased Actinobacteria and Bacteroidetes.
30473696	9	85	theme	highest	1769:1775	arg1	pH					1828:1829	the lowest gut pH	1813:1829	the lowest gut pH	1813:1829	Interestingly, pigs treated with both inulin and T. suis displayed the highest Bacteroidetes: Firmicutes ratio and the lowest gut pH, suggesting an interaction of diet and helminth infection that stimulates the growth of beneficial bacterial species.
30473696	9	85	theme	highest	1769:1775	arg1	Bacteroidetes					1777:1789	the highest Bacteroidetes	1765:1789	the highest Bacteroidetes: Firmicutes ratio and the lowest gut pH	1765:1829	Interestingly, pigs treated with both inulin and T. suis displayed the highest Bacteroidetes: Firmicutes ratio and the lowest gut pH, suggesting an interaction of diet and helminth infection that stimulates the growth of beneficial bacterial species.
30473696	9	85	theme	highest	1769:1775	arg1	ratio					1803:1807	Firmicutes ratio	1792:1807	Firmicutes ratio	1792:1807	Interestingly, pigs treated with both inulin and T. suis displayed the highest Bacteroidetes: Firmicutes ratio and the lowest gut pH, suggesting an interaction of diet and helminth infection that stimulates the growth of beneficial bacterial species.
30473696	8	86	theme	samples	1486:1492	arg1	sequencing					1449:1458	16S rRNA sequencing	1440:1458	16S rRNA sequencing of proximal colon digesta samples	1440:1492	16S rRNA sequencing of proximal colon digesta samples revealed that inulin supplementation reduced the abundance of bacterial phyla linked to inflammation, such as Proteobacteria and Firmicutes, and simultaneously increased Actinobacteria and Bacteroidetes.
30473696	5	87	theme	intestinal	970:979	arg1	remodeling					981:990	intestinal remodeling	970:990	intestinal remodeling through increased epithelial goblet and tuft cell proliferation	970:1054	T. suis infection induced a typical Th2-biased immune response characterized by transcriptional changes in Th2- and barrier function-related genes, accompanied by intestinal remodeling through increased epithelial goblet and tuft cell proliferation.
30473696	10	88	theme	anti-inflammatory	2036:2052	arg1	responses					2061:2069	anti-inflammatory immune responses	2036:2069	anti-inflammatory immune responses	2036:2069	Overall, our data demonstrate that T. suis infection and inulin co-operatively enhance anti-inflammatory immune responses, which is potentially mediated by changes in microbiota composition.
30473696	0	89	theme	Trichuris	94:102	arg1	suis					104:107	Trichuris suis	94:107	Trichuris suis	94:107	Mucosal Barrier and Th2 Immune Responses Are Enhanced by Dietary Inulin in Pigs Infected With Trichuris suis.
30473696	7	90	theme	barrier	1327:1333	arg1	genes					1335:1339	key Th2 and mucosal barrier genes	1307:1339	key Th2 and mucosal barrier genes (e.g., IL13, TFF3)	1307:1358	Notably, inulin augmented the T. suis-induced responses with increased transcription of key Th2 and mucosal barrier genes (e.g., IL13, TFF3), and synergistically suppressed pro-inflammatory genes, such as IFNG and CXCL9.
30473696	11	91	theme	infection	2268:2276	arg1	model					2278:2282	a porcine helminth infection model	2249:2282	a porcine helminth infection model	2249:2282	Our results highlight the intricate interactions between diet, immune function and microbiota composition in a porcine helminth infection model.
30473696	4	92	from	function	728:735	arg1	pigs					740:743	pigs	740:743	pigs	740:743	In this study, we investigated if dietary inulin could modulate mucosal immune function in pigs during colonization with the porcine whipworm Trichuris suis.
30473696	6	93	theme	Th2-related	1099:1109	arg1	genes					1118:1122	Th2-related immune genes	1099:1122	Th2-related immune genes (IL13, IL5)	1099:1134	We observed that inulin also up-regulated Th2-related immune genes (IL13, IL5), and suppressed Th1-related pro-inflammatory genes (IFNG, IL1A, IL8) in the colon.
30473696	11	94	theme	immune	2203:2208	arg1	function					2210:2217	immune function	2203:2217	immune function	2203:2217	Our results highlight the intricate interactions between diet, immune function and microbiota composition in a porcine helminth infection model.
30473696	4	95	theme	mucosal	713:719	arg1	function					728:735	mucosal immune function	713:735	mucosal immune function in pigs	713:743	In this study, we investigated if dietary inulin could modulate mucosal immune function in pigs during colonization with the porcine whipworm Trichuris suis.
30473696	2	96	theme	gut	361:363	arg1	function					372:379	gut immune function	361:379	gut immune function	361:379	Bioactive dietary components, such as inulin, have been extensively studied for their bioactive properties, particularly in modulating gut immune function and reducing inflammation.
30473696	0	97	theme	Th2	20:22	arg1	Responses					31:39	Th2 Immune Responses	20:39	Th2 Immune Responses	20:39	Mucosal Barrier and Th2 Immune Responses Are Enhanced by Dietary Inulin in Pigs Infected With Trichuris suis.
30473696	7	98	theme	key	1307:1309	arg1	Th2					1311:1313	key Th2	1307:1313	key Th2	1307:1313	Notably, inulin augmented the T. suis-induced responses with increased transcription of key Th2 and mucosal barrier genes (e.g., IL13, TFF3), and synergistically suppressed pro-inflammatory genes, such as IFNG and CXCL9.
30473696	9	99	theme	gut	1824:1826	arg1	Bacteroidetes					1777:1789	the highest Bacteroidetes	1765:1789	the highest Bacteroidetes: Firmicutes ratio and the lowest gut pH	1765:1829	Interestingly, pigs treated with both inulin and T. suis displayed the highest Bacteroidetes: Firmicutes ratio and the lowest gut pH, suggesting an interaction of diet and helminth infection that stimulates the growth of beneficial bacterial species.
30473696	9	99	theme	gut	1824:1826	arg1	pH					1828:1829	the lowest gut pH	1813:1829	the lowest gut pH	1813:1829	Interestingly, pigs treated with both inulin and T. suis displayed the highest Bacteroidetes: Firmicutes ratio and the lowest gut pH, suggesting an interaction of diet and helminth infection that stimulates the growth of beneficial bacterial species.
30473696	3	100	theme	gut	633:635	arg1	microbiome					637:646	the gut microbiome	629:646	the gut microbiome	629:646	It has been shown that colonization with gastrointestinal parasitic worms (helminths) may alleviate chronic inflammation through promotion of T-helper cell type (Th) 2 and T-regulatory immune responses and alterations in the gut microbiome.
30473696	5	101	theme	T.	807:808	arg1	infection					815:823	T. suis infection	807:823	T. suis infection	807:823	T. suis infection induced a typical Th2-biased immune response characterized by transcriptional changes in Th2- and barrier function-related genes, accompanied by intestinal remodeling through increased epithelial goblet and tuft cell proliferation.
30473696	3	102	theme	parasitic	466:474	arg1	helminths					483:491	helminths	483:491	helminths	483:491	It has been shown that colonization with gastrointestinal parasitic worms (helminths) may alleviate chronic inflammation through promotion of T-helper cell type (Th) 2 and T-regulatory immune responses and alterations in the gut microbiome.
30473696	3	102	theme	parasitic	466:474	arg1	worms					476:480	gastrointestinal parasitic worms	449:480	gastrointestinal parasitic worms (helminths)	449:492	It has been shown that colonization with gastrointestinal parasitic worms (helminths) may alleviate chronic inflammation through promotion of T-helper cell type (Th) 2 and T-regulatory immune responses and alterations in the gut microbiome.
30473696	5	103	theme	cell	1037:1040	arg1	proliferation					1042:1054	increased epithelial goblet and tuft cell proliferation	1000:1054	proliferation	1042:1054	T. suis infection induced a typical Th2-biased immune response characterized by transcriptional changes in Th2- and barrier function-related genes, accompanied by intestinal remodeling through increased epithelial goblet and tuft cell proliferation.
30473696	8	104	theme	phyla	1566:1570	arg1	abundance					1543:1551	the abundance	1539:1551	the abundance of bacterial phyla linked to inflammation, such as Proteobacteria and Firmicutes	1539:1632	16S rRNA sequencing of proximal colon digesta samples revealed that inulin supplementation reduced the abundance of bacterial phyla linked to inflammation, such as Proteobacteria and Firmicutes, and simultaneously increased Actinobacteria and Bacteroidetes.
30473696	2	105	theme	reducing	385:392	arg1	inflammation					394:405	reducing inflammation	385:405	reducing inflammation	385:405	Bioactive dietary components, such as inulin, have been extensively studied for their bioactive properties, particularly in modulating gut immune function and reducing inflammation.
30473696	6	106	theme	suppressed	1141:1150	arg1	genes					1181:1185	suppressed Th1-related pro-inflammatory genes	1141:1185	suppressed Th1-related pro-inflammatory genes (IFNG, IL1A, IL8)	1141:1203	We observed that inulin also up-regulated Th2-related immune genes (IL13, IL5), and suppressed Th1-related pro-inflammatory genes (IFNG, IL1A, IL8) in the colon.
30473696	2	107	theme	bioactive	312:320	arg1	properties					322:331	their bioactive properties	306:331	their bioactive properties	306:331	Bioactive dietary components, such as inulin, have been extensively studied for their bioactive properties, particularly in modulating gut immune function and reducing inflammation.
30473696	5	108	theme	epithelial	1010:1019	arg1	goblet					1021:1026	increased epithelial goblet and tuft cell proliferation	1000:1054	goblet	1021:1026	T. suis infection induced a typical Th2-biased immune response characterized by transcriptional changes in Th2- and barrier function-related genes, accompanied by intestinal remodeling through increased epithelial goblet and tuft cell proliferation.
30473696	7	109	theme	suis-induced	1252:1263	arg1	responses					1265:1273	the T. suis-induced responses	1245:1273	the T. suis-induced responses	1245:1273	Notably, inulin augmented the T. suis-induced responses with increased transcription of key Th2 and mucosal barrier genes (e.g., IL13, TFF3), and synergistically suppressed pro-inflammatory genes, such as IFNG and CXCL9.
30473696	1	110	theme	microbiota	202:211	arg1	populations					213:223	commensal gut microbiota populations	188:223	commensal gut microbiota populations	188:223	Diet composition may play a crucial role in shaping host immune responses and commensal gut microbiota populations.
30473696	5	111	theme	typical	835:841	arg1	response					861:868	a typical Th2-biased immune response	833:868	a typical Th2-biased immune response characterized by transcriptional changes in Th2- and barrier function-related genes	833:952	T. suis infection induced a typical Th2-biased immune response characterized by transcriptional changes in Th2- and barrier function-related genes, accompanied by intestinal remodeling through increased epithelial goblet and tuft cell proliferation.
30473696	10	112	theme	microbiota	2116:2125	arg1	composition					2127:2137	microbiota composition	2116:2137	microbiota composition	2116:2137	Overall, our data demonstrate that T. suis infection and inulin co-operatively enhance anti-inflammatory immune responses, which is potentially mediated by changes in microbiota composition.
30473696	6	113	theme	immune	1111:1116	arg1	genes					1118:1122	Th2-related immune genes	1099:1122	Th2-related immune genes (IL13, IL5)	1099:1134	We observed that inulin also up-regulated Th2-related immune genes (IL13, IL5), and suppressed Th1-related pro-inflammatory genes (IFNG, IL1A, IL8) in the colon.
30473696	4	114	theme	whipworm	782:789	arg1	suis					801:804	the porcine whipworm Trichuris suis	770:804	the porcine whipworm Trichuris suis	770:804	In this study, we investigated if dietary inulin could modulate mucosal immune function in pigs during colonization with the porcine whipworm Trichuris suis.
31214162	7	0	gly	deglycosylated	1401:1414	arg1	glycoproteins					1382:1394	the glycoproteins	1378:1394	the glycoproteins	1378:1394	The results showed that these hemocyanins bound to chimeric Dectin-1 and Dectin-2 receptors in vitro; which significantly decreased when the glycoproteins were deglycosylated.
31214162	6	1	from	roles	972:976	arg1	activation					1051:1060	the hemocyanin activation	1036:1060	the hemocyanin activation of murine APCs, both in dendritic cells (DCs) and macrophages	1036:1122	Thus, we studied the roles of Dectin-1, Dectin-2, and Toll-like receptor 4 (TLR4) in the hemocyanin activation of murine APCs, both in dendritic cells (DCs) and macrophages, using hemocyanins from Megathura crenulata (KLH), Concholepas concholepas (CCH) and Fissurella latimarginata (FLH).
31214162	4	2	contain	contain	752:758	arg2	domains					774:780	C-type lectin domains	760:780	C-type lectin domains	760:780	We have previously shown that hemocyanins are internalized by APCs through receptor-mediated endocytosis with proteins that contain C-type lectin domains, such as mannose receptor (MR).
31214162	4	2	contain	contain	752:758	arg1	receptor					799:806	mannose receptor	791:806	mannose receptor (MR)	791:811	We have previously shown that hemocyanins are internalized by APCs through receptor-mediated endocytosis with proteins that contain C-type lectin domains, such as mannose receptor (MR).
31214162	4	2	contain	contain	752:758	arg1	proteins					738:745	proteins	738:745	proteins	738:745	We have previously shown that hemocyanins are internalized by APCs through receptor-mediated endocytosis with proteins that contain C-type lectin domains, such as mannose receptor (MR).
31214162	13	3	with	strain	2313:2318	arg1	mutation					2341:2348	a nonfunctional mutation	2325:2348	a nonfunctional mutation for this receptor	2325:2366	The participation of TLR4 was confirmed through a decrease in IL-12p40 and IL-6 secretion induced by FLH when a TLR4 blocking antibody was used; a reduction was also observed in DCs from C3H/HeJ mice, a mouse strain with a nonfunctional mutation for this receptor.
31214162	9	4	theme	kinase	1662:1667	arg1	phosphorylation					1639:1653	phosphorylation	1639:1653	phosphorylation of Syk kinase	1639:1667	Moreover, when wild-type APCs were cultured in the presence of hemocyanins, phosphorylation of Syk kinase was not detected.
31214162	1	5	contain	have	174:177	arg2	biomedical					179:188	biomedical	179:188	biomedical	179:188	Mollusk hemocyanins have biomedical uses as carriers/adjuvants and nonspecific immunostimulants with beneficial clinical outcomes by triggering the production of proinflammatory cytokines in antigen-presenting cells (APCs) and driving immune responses toward type 1 T helper (Th1) polarization.
31214162	1	5	contain	have	174:177	arg1	hemocyanins					162:172	Mollusk hemocyanins	154:172	Mollusk hemocyanins	154:172	Mollusk hemocyanins have biomedical uses as carriers/adjuvants and nonspecific immunostimulants with beneficial clinical outcomes by triggering the production of proinflammatory cytokines in antigen-presenting cells (APCs) and driving immune responses toward type 1 T helper (Th1) polarization.
31214162	13	6	theme	TLR4	2125:2128	arg1	participation					2108:2120	The participation	2104:2120	The participation of TLR4	2104:2128	The participation of TLR4 was confirmed through a decrease in IL-12p40 and IL-6 secretion induced by FLH when a TLR4 blocking antibody was used; a reduction was also observed in DCs from C3H/HeJ mice, a mouse strain with a nonfunctional mutation for this receptor.
31214162	3	7	theme	multivalent	576:586	arg1	nature					588:593	a multivalent nature	574:593	a multivalent nature	574:593	Indeed, hemocyanins have a multivalent nature as highly mannosylated antigens.
31214162	3	7	theme	multivalent	576:586	arg1	antigens					618:625	highly mannosylated antigens	598:625	highly mannosylated antigens	598:625	Indeed, hemocyanins have a multivalent nature as highly mannosylated antigens.
31214162	13	8	theme	IL-6	2179:2182	arg1	secretion					2184:2192	IL-12p40 and IL-6 secretion	2166:2192	IL-12p40 and IL-6 secretion induced by FLH	2166:2207	The participation of TLR4 was confirmed through a decrease in IL-12p40 and IL-6 secretion induced by FLH when a TLR4 blocking antibody was used; a reduction was also observed in DCs from C3H/HeJ mice, a mouse strain with a nonfunctional mutation for this receptor.
31214162	8	9	from	effects	1461:1467	arg1	mice					1522:1525	Dectin-1 knock-out (KO) and Dectin-2 KO mice	1482:1525	mice	1522:1525	However, hemocyanin-induced proinflammatory effects in APCs from Dectin-1 knock-out (KO) and Dectin-2 KO mice were independent of both receptors.
31214162	8	9	from	effects	1461:1467	arg1	KO					1502:1503	Dectin-1 knock-out (KO) and Dectin-2 KO mice	1482:1525	KO	1502:1503	However, hemocyanin-induced proinflammatory effects in APCs from Dectin-1 knock-out (KO) and Dectin-2 KO mice were independent of both receptors.
31214162	8	9	from	effects	1461:1467	arg1	APCs					1472:1475	APCs	1472:1475	APCs from Dectin-1 knock-out (KO) and Dectin-2 KO mice	1472:1525	However, hemocyanin-induced proinflammatory effects in APCs from Dectin-1 knock-out (KO) and Dectin-2 KO mice were independent of both receptors.
31214162	12	10	theme	adapter	1964:1970	arg1	Mal					1945:1947	MyD88-adapter-like (Mal)	1925:1948	MyD88-adapter-like (Mal)	1925:1948	Moreover, DCs from mice deficient for MyD88-adapter-like (Mal), a downstream adapter molecule of TLR4, were partially activated by FLH, suggesting a role of the TLR pathway in hemocyanin recognition to activate APCs.
31214162	12	10	theme	adapter	1964:1970	arg1	molecule					1972:1979	a downstream adapter molecule	1951:1979	a downstream adapter molecule of TLR4	1951:1987	Moreover, DCs from mice deficient for MyD88-adapter-like (Mal), a downstream adapter molecule of TLR4, were partially activated by FLH, suggesting a role of the TLR pathway in hemocyanin recognition to activate APCs.
31214162	6	11	theme	receptor	1015:1022	arg1	roles					972:976	the roles	968:976	the roles of Dectin-1, Dectin-2, and Toll-like receptor 4 (TLR4) in the hemocyanin activation of murine APCs, both in dendritic cells (DCs) and macrophages	968:1122	Thus, we studied the roles of Dectin-1, Dectin-2, and Toll-like receptor 4 (TLR4) in the hemocyanin activation of murine APCs, both in dendritic cells (DCs) and macrophages, using hemocyanins from Megathura crenulata (KLH), Concholepas concholepas (CCH) and Fissurella latimarginata (FLH).
31214162	13	12	theme	nonfunctional	2327:2339	arg1	mutation					2341:2348	a nonfunctional mutation	2325:2348	a nonfunctional mutation for this receptor	2325:2366	The participation of TLR4 was confirmed through a decrease in IL-12p40 and IL-6 secretion induced by FLH when a TLR4 blocking antibody was used; a reduction was also observed in DCs from C3H/HeJ mice, a mouse strain with a nonfunctional mutation for this receptor.
31214162	0	13	theme	Antigen-Presenting	115:132	arg1	Cells					134:138	Antigen-Presenting Cells	115:138	Antigen-Presenting Cells From Mammals	115:151	TLR4, but Neither Dectin-1 nor Dectin-2, Participates in the Mollusk Hemocyanin-Induced Proinflammatory Effects in Antigen-Presenting Cells From Mammals.
31214162	1	14	theme	helper	422:427	arg1	polarization					435:446	type 1 T helper (Th1) polarization	413:446	type 1 T helper (Th1) polarization	413:446	Mollusk hemocyanins have biomedical uses as carriers/adjuvants and nonspecific immunostimulants with beneficial clinical outcomes by triggering the production of proinflammatory cytokines in antigen-presenting cells (APCs) and driving immune responses toward type 1 T helper (Th1) polarization.
31214162	3	15	theme	mannosylated	605:616	arg1	nature					588:593	a multivalent nature	574:593	a multivalent nature	574:593	Indeed, hemocyanins have a multivalent nature as highly mannosylated antigens.
31214162	3	15	theme	mannosylated	605:616	arg1	antigens					618:625	highly mannosylated antigens	598:625	highly mannosylated antigens	598:625	Indeed, hemocyanins have a multivalent nature as highly mannosylated antigens.
31214162	15	16	theme	proinflammatory	2525:2539	arg1	response					2541:2548	the hemocyanin-mediated proinflammatory response	2501:2548	the hemocyanin-mediated proinflammatory response	2501:2548	Our data showed the involvement of TLR4 in the hemocyanin-mediated proinflammatory response in APCs, which could cooperate with MR in innate immune recognition of these glycoproteins.
31214162	5	17	theme	signaling	896:904	arg1	pathway					906:912	the proinflammatory signaling pathway	876:912	the proinflammatory signaling pathway triggered by hemocyanins	876:937	However, the contribution of other innate immune receptors to the proinflammatory signaling pathway triggered by hemocyanins is unknown.
31214162	7	18	gly	glycoproteins	1382:1394	arg1	glycoproteins					1382:1394	the glycoproteins	1378:1394	the glycoproteins	1378:1394	The results showed that these hemocyanins bound to chimeric Dectin-1 and Dectin-2 receptors in vitro; which significantly decreased when the glycoproteins were deglycosylated.
31214162	4	19	theme	receptor-mediated	703:719	arg1	endocytosis					721:731	receptor-mediated endocytosis	703:731	receptor-mediated endocytosis with proteins that contain C-type lectin domains, such as mannose receptor (MR)	703:811	We have previously shown that hemocyanins are internalized by APCs through receptor-mediated endocytosis with proteins that contain C-type lectin domains, such as mannose receptor (MR).
31214162	6	20	theme	Dectin-2	991:998	arg1	roles					972:976	the roles	968:976	the roles of Dectin-1, Dectin-2, and Toll-like receptor 4 (TLR4) in the hemocyanin activation of murine APCs, both in dendritic cells (DCs) and macrophages	968:1122	Thus, we studied the roles of Dectin-1, Dectin-2, and Toll-like receptor 4 (TLR4) in the hemocyanin activation of murine APCs, both in dendritic cells (DCs) and macrophages, using hemocyanins from Megathura crenulata (KLH), Concholepas concholepas (CCH) and Fissurella latimarginata (FLH).
31214162	10	21	theme	TLR	1782:1784	arg1	pathway					1796:1802	the TLR signaling pathway	1778:1802	the TLR signaling pathway	1778:1802	We further showed that KLH and FLH induced ERK1/2 phosphorylation, a key event involved in the TLR signaling pathway.
31214162	6	22	theme	Dectin-1	981:988	arg1	roles					972:976	the roles	968:976	the roles of Dectin-1, Dectin-2, and Toll-like receptor 4 (TLR4) in the hemocyanin activation of murine APCs, both in dendritic cells (DCs) and macrophages	968:1122	Thus, we studied the roles of Dectin-1, Dectin-2, and Toll-like receptor 4 (TLR4) in the hemocyanin activation of murine APCs, both in dendritic cells (DCs) and macrophages, using hemocyanins from Megathura crenulata (KLH), Concholepas concholepas (CCH) and Fissurella latimarginata (FLH).
31214162	8	23	theme	knock-out	1491:1499	arg1	KO					1502:1503	Dectin-1 knock-out (KO) and Dectin-2 KO mice	1482:1525	KO	1502:1503	However, hemocyanin-induced proinflammatory effects in APCs from Dectin-1 knock-out (KO) and Dectin-2 KO mice were independent of both receptors.
31214162	12	24	theme	TLR	2048:2050	arg1	pathway					2052:2058	the TLR pathway	2044:2058	the TLR pathway	2044:2058	Moreover, DCs from mice deficient for MyD88-adapter-like (Mal), a downstream adapter molecule of TLR4, were partially activated by FLH, suggesting a role of the TLR pathway in hemocyanin recognition to activate APCs.
31214162	6	25	from	Fissurella	1209:1218	arg1	hemocyanins					1131:1141	hemocyanins	1131:1141	hemocyanins from Megathura crenulata (KLH), Concholepas concholepas (CCH) and Fissurella latimarginata (FLH)	1131:1238	Thus, we studied the roles of Dectin-1, Dectin-2, and Toll-like receptor 4 (TLR4) in the hemocyanin activation of murine APCs, both in dendritic cells (DCs) and macrophages, using hemocyanins from Megathura crenulata (KLH), Concholepas concholepas (CCH) and Fissurella latimarginata (FLH).
31214162	4	26	theme	mannose	791:797	arg1	MR					809:810	MR	809:810	MR	809:810	We have previously shown that hemocyanins are internalized by APCs through receptor-mediated endocytosis with proteins that contain C-type lectin domains, such as mannose receptor (MR).
31214162	4	26	theme	mannose	791:797	arg1	receptor					799:806	mannose receptor	791:806	mannose receptor (MR)	791:811	We have previously shown that hemocyanins are internalized by APCs through receptor-mediated endocytosis with proteins that contain C-type lectin domains, such as mannose receptor (MR).
31214162	4	27	theme	C-type	760:765	arg1	domains					774:780	C-type lectin domains	760:780	C-type lectin domains	760:780	We have previously shown that hemocyanins are internalized by APCs through receptor-mediated endocytosis with proteins that contain C-type lectin domains, such as mannose receptor (MR).
31214162	1	28	theme	Mollusk	154:160	arg1	hemocyanins					162:172	Mollusk hemocyanins	154:172	Mollusk hemocyanins	154:172	Mollusk hemocyanins have biomedical uses as carriers/adjuvants and nonspecific immunostimulants with beneficial clinical outcomes by triggering the production of proinflammatory cytokines in antigen-presenting cells (APCs) and driving immune responses toward type 1 T helper (Th1) polarization.
31214162	2	29	theme	glycosylation	525:537	arg1	patterns					539:546	their glycosylation patterns	519:546	their glycosylation patterns	519:546	Significant structural features of hemocyanins as a model antigen are their glycosylation patterns.
31214162	2	29	theme	glycosylation	525:537	arg1	features					472:479	Significant structural features	449:479	Significant structural features of hemocyanins as a model antigen	449:513	Significant structural features of hemocyanins as a model antigen are their glycosylation patterns.
31214162	1	30	theme	immune	389:394	arg1	responses					396:404	immune responses	389:404	immune responses toward type 1 T helper (Th1) polarization	389:446	Mollusk hemocyanins have biomedical uses as carriers/adjuvants and nonspecific immunostimulants with beneficial clinical outcomes by triggering the production of proinflammatory cytokines in antigen-presenting cells (APCs) and driving immune responses toward type 1 T helper (Th1) polarization.
31214162	13	31	theme	TLR4	2216:2219	arg1	antibody					2230:2237	a TLR4 blocking antibody	2214:2237	a TLR4 blocking antibody	2214:2237	The participation of TLR4 was confirmed through a decrease in IL-12p40 and IL-6 secretion induced by FLH when a TLR4 blocking antibody was used; a reduction was also observed in DCs from C3H/HeJ mice, a mouse strain with a nonfunctional mutation for this receptor.
31214162	8	32	theme	KO	1519:1520	arg1	mice					1522:1525	Dectin-1 knock-out (KO) and Dectin-2 KO mice	1482:1525	mice	1522:1525	However, hemocyanin-induced proinflammatory effects in APCs from Dectin-1 knock-out (KO) and Dectin-2 KO mice were independent of both receptors.
31214162	13	33	from	decrease	2154:2161	arg1	secretion					2184:2192	IL-12p40 and IL-6 secretion	2166:2192	IL-12p40 and IL-6 secretion induced by FLH	2166:2207	The participation of TLR4 was confirmed through a decrease in IL-12p40 and IL-6 secretion induced by FLH when a TLR4 blocking antibody was used; a reduction was also observed in DCs from C3H/HeJ mice, a mouse strain with a nonfunctional mutation for this receptor.
31214162	6	34	dep	APCs	1072:1075	arg1	both					1078:1081	both	1078:1081	both	1078:1081	Thus, we studied the roles of Dectin-1, Dectin-2, and Toll-like receptor 4 (TLR4) in the hemocyanin activation of murine APCs, both in dendritic cells (DCs) and macrophages, using hemocyanins from Megathura crenulata (KLH), Concholepas concholepas (CCH) and Fissurella latimarginata (FLH).
31214162	0	35	theme	Hemocyanin-Induced	69:86	arg1	Effects					104:110	the Mollusk Hemocyanin-Induced Proinflammatory Effects	57:110	the Mollusk Hemocyanin-Induced Proinflammatory Effects	57:110	TLR4, but Neither Dectin-1 nor Dectin-2, Participates in the Mollusk Hemocyanin-Induced Proinflammatory Effects in Antigen-Presenting Cells From Mammals.
31214162	12	36	theme	deficient	1911:1919	arg1	mice					1906:1909	mice	1906:1909	mice deficient for MyD88-adapter-like (Mal), a downstream adapter molecule of TLR4,	1906:1988	Moreover, DCs from mice deficient for MyD88-adapter-like (Mal), a downstream adapter molecule of TLR4, were partially activated by FLH, suggesting a role of the TLR pathway in hemocyanin recognition to activate APCs.
31214162	15	37	from	involvement	2478:2488	arg1	response					2541:2548	the hemocyanin-mediated proinflammatory response	2501:2548	the hemocyanin-mediated proinflammatory response	2501:2548	Our data showed the involvement of TLR4 in the hemocyanin-mediated proinflammatory response in APCs, which could cooperate with MR in innate immune recognition of these glycoproteins.
31214162	6	38	from	Concholepas	1175:1185	arg1	hemocyanins					1131:1141	hemocyanins	1131:1141	hemocyanins from Megathura crenulata (KLH), Concholepas concholepas (CCH) and Fissurella latimarginata (FLH)	1131:1238	Thus, we studied the roles of Dectin-1, Dectin-2, and Toll-like receptor 4 (TLR4) in the hemocyanin activation of murine APCs, both in dendritic cells (DCs) and macrophages, using hemocyanins from Megathura crenulata (KLH), Concholepas concholepas (CCH) and Fissurella latimarginata (FLH).
31214162	5	39	theme	receptors	863:871	arg1	contribution					827:838	the contribution	823:838	the contribution of other innate immune receptors to the proinflammatory signaling pathway triggered by hemocyanins	823:937	However, the contribution of other innate immune receptors to the proinflammatory signaling pathway triggered by hemocyanins is unknown.
31214162	5	39	theme	receptors	863:871	arg1	unknown					942:948	unknown	942:948	unknown	942:948	However, the contribution of other innate immune receptors to the proinflammatory signaling pathway triggered by hemocyanins is unknown.
31214162	1	40	theme	proinflammatory	316:330	arg1	cytokines					332:340	proinflammatory cytokines	316:340	proinflammatory cytokines	316:340	Mollusk hemocyanins have biomedical uses as carriers/adjuvants and nonspecific immunostimulants with beneficial clinical outcomes by triggering the production of proinflammatory cytokines in antigen-presenting cells (APCs) and driving immune responses toward type 1 T helper (Th1) polarization.
31214162	9	41	theme	wild-type	1578:1586	arg1	APCs					1588:1591	wild-type APCs	1578:1591	wild-type APCs	1578:1591	Moreover, when wild-type APCs were cultured in the presence of hemocyanins, phosphorylation of Syk kinase was not detected.
31214162	7	42	theme	Dectin-2	1314:1321	arg1	receptors					1323:1331	chimeric Dectin-1 and Dectin-2 receptors	1292:1331	chimeric Dectin-1 and Dectin-2 receptors	1292:1331	The results showed that these hemocyanins bound to chimeric Dectin-1 and Dectin-2 receptors in vitro; which significantly decreased when the glycoproteins were deglycosylated.
31214162	8	43	theme	receptors	1552:1560	arg1	independent					1532:1542	independent	1532:1542	independent	1532:1542	However, hemocyanin-induced proinflammatory effects in APCs from Dectin-1 knock-out (KO) and Dectin-2 KO mice were independent of both receptors.
31214162	6	44	from	Megathura	1148:1156	arg1	hemocyanins					1131:1141	hemocyanins	1131:1141	hemocyanins from Megathura crenulata (KLH), Concholepas concholepas (CCH) and Fissurella latimarginata (FLH)	1131:1238	Thus, we studied the roles of Dectin-1, Dectin-2, and Toll-like receptor 4 (TLR4) in the hemocyanin activation of murine APCs, both in dendritic cells (DCs) and macrophages, using hemocyanins from Megathura crenulata (KLH), Concholepas concholepas (CCH) and Fissurella latimarginata (FLH).
31214162	2	45	theme	structural	461:470	arg1	patterns					539:546	their glycosylation patterns	519:546	their glycosylation patterns	519:546	Significant structural features of hemocyanins as a model antigen are their glycosylation patterns.
31214162	2	45	theme	structural	461:470	arg1	features					472:479	Significant structural features	449:479	Significant structural features of hemocyanins as a model antigen	449:513	Significant structural features of hemocyanins as a model antigen are their glycosylation patterns.
31214162	7	46	theme	Dectin-1	1301:1308	arg1	receptors					1323:1331	chimeric Dectin-1 and Dectin-2 receptors	1292:1331	chimeric Dectin-1 and Dectin-2 receptors	1292:1331	The results showed that these hemocyanins bound to chimeric Dectin-1 and Dectin-2 receptors in vitro; which significantly decreased when the glycoproteins were deglycosylated.
31214162	15	47	theme	innate	2592:2597	arg1	recognition					2606:2616	innate immune recognition	2592:2616	innate immune recognition of these glycoproteins	2592:2639	Our data showed the involvement of TLR4 in the hemocyanin-mediated proinflammatory response in APCs, which could cooperate with MR in innate immune recognition of these glycoproteins.
31214162	12	48	from	mice	1906:1909	arg1	DCs					1897:1899	DCs	1897:1899	DCs from mice deficient for MyD88-adapter-like (Mal), a downstream adapter molecule of TLR4,	1897:1988	Moreover, DCs from mice deficient for MyD88-adapter-like (Mal), a downstream adapter molecule of TLR4, were partially activated by FLH, suggesting a role of the TLR pathway in hemocyanin recognition to activate APCs.
31214162	6	49	theme	murine	1065:1070	arg1	APCs					1072:1075	murine APCs	1065:1075	murine APCs	1065:1075	Thus, we studied the roles of Dectin-1, Dectin-2, and Toll-like receptor 4 (TLR4) in the hemocyanin activation of murine APCs, both in dendritic cells (DCs) and macrophages, using hemocyanins from Megathura crenulata (KLH), Concholepas concholepas (CCH) and Fissurella latimarginata (FLH).
31214162	2	50	theme	model	501:505	arg1	antigen					507:513	a model antigen	499:513	a model antigen	499:513	Significant structural features of hemocyanins as a model antigen are their glycosylation patterns.
31214162	4	51	with	endocytosis	721:731	arg1	receptor					799:806	mannose receptor	791:806	mannose receptor (MR)	791:811	We have previously shown that hemocyanins are internalized by APCs through receptor-mediated endocytosis with proteins that contain C-type lectin domains, such as mannose receptor (MR).
31214162	4	51	with	endocytosis	721:731	arg1	proteins					738:745	proteins	738:745	proteins	738:745	We have previously shown that hemocyanins are internalized by APCs through receptor-mediated endocytosis with proteins that contain C-type lectin domains, such as mannose receptor (MR).
31214162	1	52	dep	biomedical	179:188	arg1	uses					190:193	uses	190:193	uses	190:193	Mollusk hemocyanins have biomedical uses as carriers/adjuvants and nonspecific immunostimulants with beneficial clinical outcomes by triggering the production of proinflammatory cytokines in antigen-presenting cells (APCs) and driving immune responses toward type 1 T helper (Th1) polarization.
31214162	11	53	theme	hemocyanins	1848:1858	arg1	binding					1837:1843	a glycan-dependent binding	1818:1843	a glycan-dependent binding of hemocyanins to chimeric TLR4	1818:1875	We confirmed a glycan-dependent binding of hemocyanins to chimeric TLR4 in vitro.
31214162	5	54	theme	innate	849:854	arg1	receptors					863:871	other innate immune receptors	843:871	other innate immune receptors	843:871	However, the contribution of other innate immune receptors to the proinflammatory signaling pathway triggered by hemocyanins is unknown.
31214162	13	55	theme	IL-12p40	2166:2173	arg1	secretion					2184:2192	IL-12p40 and IL-6 secretion	2166:2192	IL-12p40 and IL-6 secretion induced by FLH	2166:2207	The participation of TLR4 was confirmed through a decrease in IL-12p40 and IL-6 secretion induced by FLH when a TLR4 blocking antibody was used; a reduction was also observed in DCs from C3H/HeJ mice, a mouse strain with a nonfunctional mutation for this receptor.
31214162	1	56	with	carriers/adjuvants	198:215	arg1	outcomes					275:282	beneficial clinical outcomes	255:282	beneficial clinical outcomes	255:282	Mollusk hemocyanins have biomedical uses as carriers/adjuvants and nonspecific immunostimulants with beneficial clinical outcomes by triggering the production of proinflammatory cytokines in antigen-presenting cells (APCs) and driving immune responses toward type 1 T helper (Th1) polarization.
31214162	8	57	theme	hemocyanin-induced	1426:1443	arg1	effects					1461:1467	hemocyanin-induced proinflammatory effects	1426:1467	hemocyanin-induced proinflammatory effects in APCs from Dectin-1 knock-out (KO) and Dectin-2 KO mice	1426:1525	However, hemocyanin-induced proinflammatory effects in APCs from Dectin-1 knock-out (KO) and Dectin-2 KO mice were independent of both receptors.
31214162	13	58	theme	mouse	2307:2311	arg1	mice					2299:2302	C3H/HeJ mice	2291:2302	C3H/HeJ mice	2291:2302	The participation of TLR4 was confirmed through a decrease in IL-12p40 and IL-6 secretion induced by FLH when a TLR4 blocking antibody was used; a reduction was also observed in DCs from C3H/HeJ mice, a mouse strain with a nonfunctional mutation for this receptor.
31214162	13	58	theme	mouse	2307:2311	arg1	strain					2313:2318	a mouse strain	2305:2318	a mouse strain with a nonfunctional mutation for this receptor	2305:2366	The participation of TLR4 was confirmed through a decrease in IL-12p40 and IL-6 secretion induced by FLH when a TLR4 blocking antibody was used; a reduction was also observed in DCs from C3H/HeJ mice, a mouse strain with a nonfunctional mutation for this receptor.
31214162	3	59	gly	mannosylated	605:616	arg1	nature					588:593	a multivalent nature	574:593	a multivalent nature	574:593	Indeed, hemocyanins have a multivalent nature as highly mannosylated antigens.
31214162	3	59	gly	mannosylated	605:616	arg1	antigens					618:625	highly mannosylated antigens	598:625	highly mannosylated antigens	598:625	Indeed, hemocyanins have a multivalent nature as highly mannosylated antigens.
31214162	1	60	theme	beneficial	255:264	arg1	outcomes					275:282	beneficial clinical outcomes	255:282	beneficial clinical outcomes	255:282	Mollusk hemocyanins have biomedical uses as carriers/adjuvants and nonspecific immunostimulants with beneficial clinical outcomes by triggering the production of proinflammatory cytokines in antigen-presenting cells (APCs) and driving immune responses toward type 1 T helper (Th1) polarization.
31214162	9	61	theme	hemocyanins	1626:1636	arg1	presence					1614:1621	the presence	1610:1621	the presence of hemocyanins	1610:1636	Moreover, when wild-type APCs were cultured in the presence of hemocyanins, phosphorylation of Syk kinase was not detected.
31214162	10	62	theme	ERK1/2	1730:1735	arg1	phosphorylation					1737:1751	ERK1/2 phosphorylation	1730:1751	ERK1/2 phosphorylation	1730:1751	We further showed that KLH and FLH induced ERK1/2 phosphorylation, a key event involved in the TLR signaling pathway.
31214162	10	62	theme	ERK1/2	1730:1735	arg1	event					1760:1764	a key event	1754:1764	a key event involved in the TLR signaling pathway	1754:1802	We further showed that KLH and FLH induced ERK1/2 phosphorylation, a key event involved in the TLR signaling pathway.
31214162	15	63	theme	glycoproteins	2627:2639	arg1	recognition					2606:2616	innate immune recognition	2592:2616	innate immune recognition of these glycoproteins	2592:2639	Our data showed the involvement of TLR4 in the hemocyanin-mediated proinflammatory response in APCs, which could cooperate with MR in innate immune recognition of these glycoproteins.
31214162	15	64	theme	immune	2599:2604	arg1	recognition					2606:2616	innate immune recognition	2592:2616	innate immune recognition of these glycoproteins	2592:2639	Our data showed the involvement of TLR4 in the hemocyanin-mediated proinflammatory response in APCs, which could cooperate with MR in innate immune recognition of these glycoproteins.
31214162	13	65	located	observed	2270:2277	arg2	reduction					2251:2259	a reduction	2249:2259	a reduction	2249:2259	The participation of TLR4 was confirmed through a decrease in IL-12p40 and IL-6 secretion induced by FLH when a TLR4 blocking antibody was used; a reduction was also observed in DCs from C3H/HeJ mice, a mouse strain with a nonfunctional mutation for this receptor.
31214162	13	65	located	observed	2270:2277	arg1	DCs					2282:2284	DCs	2282:2284	DCs from C3H/HeJ mice, a mouse strain with a nonfunctional mutation for this receptor	2282:2366	The participation of TLR4 was confirmed through a decrease in IL-12p40 and IL-6 secretion induced by FLH when a TLR4 blocking antibody was used; a reduction was also observed in DCs from C3H/HeJ mice, a mouse strain with a nonfunctional mutation for this receptor.
31214162	11	66	theme	chimeric	1863:1870	arg1	TLR4					1872:1875	chimeric TLR4	1863:1875	chimeric TLR4	1863:1875	We confirmed a glycan-dependent binding of hemocyanins to chimeric TLR4 in vitro.
31214162	9	67	theme	Syk	1658:1660	arg1	kinase					1662:1667	Syk kinase	1658:1667	Syk kinase	1658:1667	Moreover, when wild-type APCs were cultured in the presence of hemocyanins, phosphorylation of Syk kinase was not detected.
31214162	10	68	theme	key	1756:1758	arg1	phosphorylation					1737:1751	ERK1/2 phosphorylation	1730:1751	ERK1/2 phosphorylation	1730:1751	We further showed that KLH and FLH induced ERK1/2 phosphorylation, a key event involved in the TLR signaling pathway.
31214162	10	68	theme	key	1756:1758	arg1	event					1760:1764	a key event	1754:1764	a key event involved in the TLR signaling pathway	1754:1802	We further showed that KLH and FLH induced ERK1/2 phosphorylation, a key event involved in the TLR signaling pathway.
31214162	12	69	theme	TLR4	1984:1987	arg1	Mal					1945:1947	MyD88-adapter-like (Mal)	1925:1948	MyD88-adapter-like (Mal)	1925:1948	Moreover, DCs from mice deficient for MyD88-adapter-like (Mal), a downstream adapter molecule of TLR4, were partially activated by FLH, suggesting a role of the TLR pathway in hemocyanin recognition to activate APCs.
31214162	12	69	theme	TLR4	1984:1987	arg1	molecule					1972:1979	a downstream adapter molecule	1951:1979	a downstream adapter molecule of TLR4	1951:1987	Moreover, DCs from mice deficient for MyD88-adapter-like (Mal), a downstream adapter molecule of TLR4, were partially activated by FLH, suggesting a role of the TLR pathway in hemocyanin recognition to activate APCs.
31214162	14	70	theme	IL-6	2379:2382	arg1	secretion					2384:2392	IL-6 secretion	2379:2392	IL-6 secretion induced by FLH	2379:2407	Moreover, IL-6 secretion induced by FLH was abolished in macrophages deficient for TLR4.
31214162	13	71	used	used	2243:2246	arg2	antibody					2230:2237	a TLR4 blocking antibody	2214:2237	a TLR4 blocking antibody	2214:2237	The participation of TLR4 was confirmed through a decrease in IL-12p40 and IL-6 secretion induced by FLH when a TLR4 blocking antibody was used; a reduction was also observed in DCs from C3H/HeJ mice, a mouse strain with a nonfunctional mutation for this receptor.
31214162	6	72	dep	Megathura	1148:1156	arg1	crenulata					1158:1166	Megathura crenulata (KLH)	1148:1172	Megathura crenulata (KLH)	1148:1172	Thus, we studied the roles of Dectin-1, Dectin-2, and Toll-like receptor 4 (TLR4) in the hemocyanin activation of murine APCs, both in dendritic cells (DCs) and macrophages, using hemocyanins from Megathura crenulata (KLH), Concholepas concholepas (CCH) and Fissurella latimarginata (FLH).
31214162	5	73	theme	proinflammatory	880:894	arg1	pathway					906:912	the proinflammatory signaling pathway	876:912	the proinflammatory signaling pathway triggered by hemocyanins	876:937	However, the contribution of other innate immune receptors to the proinflammatory signaling pathway triggered by hemocyanins is unknown.
31214162	12	74	theme	downstream	1953:1962	arg1	Mal					1945:1947	MyD88-adapter-like (Mal)	1925:1948	MyD88-adapter-like (Mal)	1925:1948	Moreover, DCs from mice deficient for MyD88-adapter-like (Mal), a downstream adapter molecule of TLR4, were partially activated by FLH, suggesting a role of the TLR pathway in hemocyanin recognition to activate APCs.
31214162	12	74	theme	downstream	1953:1962	arg1	molecule					1972:1979	a downstream adapter molecule	1951:1979	a downstream adapter molecule of TLR4	1951:1987	Moreover, DCs from mice deficient for MyD88-adapter-like (Mal), a downstream adapter molecule of TLR4, were partially activated by FLH, suggesting a role of the TLR pathway in hemocyanin recognition to activate APCs.
31214162	6	75	theme	Toll-like	1005:1013	arg1	receptor					1015:1022	Toll-like receptor 4	1005:1024	Toll-like receptor 4 (TLR4)	1005:1031	Thus, we studied the roles of Dectin-1, Dectin-2, and Toll-like receptor 4 (TLR4) in the hemocyanin activation of murine APCs, both in dendritic cells (DCs) and macrophages, using hemocyanins from Megathura crenulata (KLH), Concholepas concholepas (CCH) and Fissurella latimarginata (FLH).
31214162	6	75	theme	Toll-like	1005:1013	arg1	TLR4					1027:1030	TLR4	1027:1030	TLR4	1027:1030	Thus, we studied the roles of Dectin-1, Dectin-2, and Toll-like receptor 4 (TLR4) in the hemocyanin activation of murine APCs, both in dendritic cells (DCs) and macrophages, using hemocyanins from Megathura crenulata (KLH), Concholepas concholepas (CCH) and Fissurella latimarginata (FLH).
31214162	12	76	theme	pathway	2052:2058	arg1	role					2036:2039	a role	2034:2039	a role of the TLR pathway in hemocyanin recognition	2034:2084	Moreover, DCs from mice deficient for MyD88-adapter-like (Mal), a downstream adapter molecule of TLR4, were partially activated by FLH, suggesting a role of the TLR pathway in hemocyanin recognition to activate APCs.
31214162	12	77	theme	MyD88-adapter-like	1925:1942	arg1	Mal					1945:1947	MyD88-adapter-like (Mal)	1925:1948	MyD88-adapter-like (Mal)	1925:1948	Moreover, DCs from mice deficient for MyD88-adapter-like (Mal), a downstream adapter molecule of TLR4, were partially activated by FLH, suggesting a role of the TLR pathway in hemocyanin recognition to activate APCs.
31214162	12	77	theme	MyD88-adapter-like	1925:1942	arg1	molecule					1972:1979	a downstream adapter molecule	1951:1979	a downstream adapter molecule of TLR4	1951:1987	Moreover, DCs from mice deficient for MyD88-adapter-like (Mal), a downstream adapter molecule of TLR4, were partially activated by FLH, suggesting a role of the TLR pathway in hemocyanin recognition to activate APCs.
31214162	8	78	theme	Dectin-1	1482:1489	arg1	KO					1502:1503	Dectin-1 knock-out (KO) and Dectin-2 KO mice	1482:1525	KO	1502:1503	However, hemocyanin-induced proinflammatory effects in APCs from Dectin-1 knock-out (KO) and Dectin-2 KO mice were independent of both receptors.
31214162	12	79	theme	hemocyanin	2063:2072	arg1	recognition					2074:2084	hemocyanin recognition	2063:2084	hemocyanin recognition	2063:2084	Moreover, DCs from mice deficient for MyD88-adapter-like (Mal), a downstream adapter molecule of TLR4, were partially activated by FLH, suggesting a role of the TLR pathway in hemocyanin recognition to activate APCs.
31214162	11	80	theme	glycan-dependent	1820:1835	arg1	binding					1837:1843	a glycan-dependent binding	1818:1843	a glycan-dependent binding of hemocyanins to chimeric TLR4	1818:1875	We confirmed a glycan-dependent binding of hemocyanins to chimeric TLR4 in vitro.
31214162	10	81	theme	signaling	1786:1794	arg1	pathway					1796:1802	the TLR signaling pathway	1778:1802	the TLR signaling pathway	1778:1802	We further showed that KLH and FLH induced ERK1/2 phosphorylation, a key event involved in the TLR signaling pathway.
31214162	13	82	theme	blocking	2221:2228	arg1	antibody					2230:2237	a TLR4 blocking antibody	2214:2237	a TLR4 blocking antibody	2214:2237	The participation of TLR4 was confirmed through a decrease in IL-12p40 and IL-6 secretion induced by FLH when a TLR4 blocking antibody was used; a reduction was also observed in DCs from C3H/HeJ mice, a mouse strain with a nonfunctional mutation for this receptor.
31214162	12	83	from	role	2036:2039	arg1	recognition					2074:2084	hemocyanin recognition	2063:2084	hemocyanin recognition	2063:2084	Moreover, DCs from mice deficient for MyD88-adapter-like (Mal), a downstream adapter molecule of TLR4, were partially activated by FLH, suggesting a role of the TLR pathway in hemocyanin recognition to activate APCs.
31214162	15	84	theme	hemocyanin-mediated	2505:2523	arg1	response					2541:2548	the hemocyanin-mediated proinflammatory response	2501:2548	the hemocyanin-mediated proinflammatory response	2501:2548	Our data showed the involvement of TLR4 in the hemocyanin-mediated proinflammatory response in APCs, which could cooperate with MR in innate immune recognition of these glycoproteins.
31214162	6	85	theme	hemocyanin	1040:1049	arg1	activation					1051:1060	the hemocyanin activation	1036:1060	the hemocyanin activation of murine APCs, both in dendritic cells (DCs) and macrophages	1036:1122	Thus, we studied the roles of Dectin-1, Dectin-2, and Toll-like receptor 4 (TLR4) in the hemocyanin activation of murine APCs, both in dendritic cells (DCs) and macrophages, using hemocyanins from Megathura crenulata (KLH), Concholepas concholepas (CCH) and Fissurella latimarginata (FLH).
31214162	8	86	theme	Dectin-2	1510:1517	arg1	mice					1522:1525	Dectin-1 knock-out (KO) and Dectin-2 KO mice	1482:1525	mice	1522:1525	However, hemocyanin-induced proinflammatory effects in APCs from Dectin-1 knock-out (KO) and Dectin-2 KO mice were independent of both receptors.
31214162	6	87	dep	Fissurella	1209:1218	arg1	latimarginata					1220:1232	Fissurella latimarginata (FLH)	1209:1238	Fissurella latimarginata (FLH)	1209:1238	Thus, we studied the roles of Dectin-1, Dectin-2, and Toll-like receptor 4 (TLR4) in the hemocyanin activation of murine APCs, both in dendritic cells (DCs) and macrophages, using hemocyanins from Megathura crenulata (KLH), Concholepas concholepas (CCH) and Fissurella latimarginata (FLH).
31214162	8	88	from	KO	1502:1503	arg1	APCs					1472:1475	APCs	1472:1475	APCs from Dectin-1 knock-out (KO) and Dectin-2 KO mice	1472:1525	However, hemocyanin-induced proinflammatory effects in APCs from Dectin-1 knock-out (KO) and Dectin-2 KO mice were independent of both receptors.
31214162	8	88	from	KO	1502:1503	arg1	effects					1461:1467	hemocyanin-induced proinflammatory effects	1426:1467	hemocyanin-induced proinflammatory effects in APCs from Dectin-1 knock-out (KO) and Dectin-2 KO mice	1426:1525	However, hemocyanin-induced proinflammatory effects in APCs from Dectin-1 knock-out (KO) and Dectin-2 KO mice were independent of both receptors.
31214162	0	89	theme	Mollusk	61:67	arg1	Effects					104:110	the Mollusk Hemocyanin-Induced Proinflammatory Effects	57:110	the Mollusk Hemocyanin-Induced Proinflammatory Effects	57:110	TLR4, but Neither Dectin-1 nor Dectin-2, Participates in the Mollusk Hemocyanin-Induced Proinflammatory Effects in Antigen-Presenting Cells From Mammals.
31214162	3	90	contain	have	569:572	arg2	antigens					618:625	highly mannosylated antigens	598:625	highly mannosylated antigens	598:625	Indeed, hemocyanins have a multivalent nature as highly mannosylated antigens.
31214162	3	90	contain	have	569:572	arg1	hemocyanins					557:567	hemocyanins	557:567	hemocyanins	557:567	Indeed, hemocyanins have a multivalent nature as highly mannosylated antigens.
31214162	3	90	contain	have	569:572	arg2	nature					588:593	a multivalent nature	574:593	a multivalent nature	574:593	Indeed, hemocyanins have a multivalent nature as highly mannosylated antigens.
31214162	4	91	theme	lectin	767:772	arg1	domains					774:780	C-type lectin domains	760:780	C-type lectin domains	760:780	We have previously shown that hemocyanins are internalized by APCs through receptor-mediated endocytosis with proteins that contain C-type lectin domains, such as mannose receptor (MR).
31214162	0	92	theme	Proinflammatory	88:102	arg1	Effects					104:110	the Mollusk Hemocyanin-Induced Proinflammatory Effects	57:110	the Mollusk Hemocyanin-Induced Proinflammatory Effects	57:110	TLR4, but Neither Dectin-1 nor Dectin-2, Participates in the Mollusk Hemocyanin-Induced Proinflammatory Effects in Antigen-Presenting Cells From Mammals.
31214162	13	93	theme	C3H/HeJ	2291:2297	arg1	mice					2299:2302	C3H/HeJ mice	2291:2302	C3H/HeJ mice	2291:2302	The participation of TLR4 was confirmed through a decrease in IL-12p40 and IL-6 secretion induced by FLH when a TLR4 blocking antibody was used; a reduction was also observed in DCs from C3H/HeJ mice, a mouse strain with a nonfunctional mutation for this receptor.
31214162	13	93	theme	C3H/HeJ	2291:2297	arg1	strain					2313:2318	a mouse strain	2305:2318	a mouse strain with a nonfunctional mutation for this receptor	2305:2366	The participation of TLR4 was confirmed through a decrease in IL-12p40 and IL-6 secretion induced by FLH when a TLR4 blocking antibody was used; a reduction was also observed in DCs from C3H/HeJ mice, a mouse strain with a nonfunctional mutation for this receptor.
31214162	13	94	from	mice	2299:2302	arg1	DCs					2282:2284	DCs	2282:2284	DCs from C3H/HeJ mice, a mouse strain with a nonfunctional mutation for this receptor	2282:2366	The participation of TLR4 was confirmed through a decrease in IL-12p40 and IL-6 secretion induced by FLH when a TLR4 blocking antibody was used; a reduction was also observed in DCs from C3H/HeJ mice, a mouse strain with a nonfunctional mutation for this receptor.
31214162	1	95	theme	type	413:416	arg1	Th1					430:432	Th1	430:432	Th1	430:432	Mollusk hemocyanins have biomedical uses as carriers/adjuvants and nonspecific immunostimulants with beneficial clinical outcomes by triggering the production of proinflammatory cytokines in antigen-presenting cells (APCs) and driving immune responses toward type 1 T helper (Th1) polarization.
31214162	1	95	theme	type	413:416	arg1	helper					422:427	type 1 T helper	413:427	type 1 T helper (Th1) polarization	413:446	Mollusk hemocyanins have biomedical uses as carriers/adjuvants and nonspecific immunostimulants with beneficial clinical outcomes by triggering the production of proinflammatory cytokines in antigen-presenting cells (APCs) and driving immune responses toward type 1 T helper (Th1) polarization.
31214162	1	96	theme	nonspecific	221:231	arg1	immunostimulants					233:248	nonspecific immunostimulants	221:248	nonspecific immunostimulants	221:248	Mollusk hemocyanins have biomedical uses as carriers/adjuvants and nonspecific immunostimulants with beneficial clinical outcomes by triggering the production of proinflammatory cytokines in antigen-presenting cells (APCs) and driving immune responses toward type 1 T helper (Th1) polarization.
31214162	5	97	theme	immune	856:861	arg1	receptors					863:871	other innate immune receptors	843:871	other innate immune receptors	843:871	However, the contribution of other innate immune receptors to the proinflammatory signaling pathway triggered by hemocyanins is unknown.
31214162	1	98	theme	T	420:420	arg1	Th1					430:432	Th1	430:432	Th1	430:432	Mollusk hemocyanins have biomedical uses as carriers/adjuvants and nonspecific immunostimulants with beneficial clinical outcomes by triggering the production of proinflammatory cytokines in antigen-presenting cells (APCs) and driving immune responses toward type 1 T helper (Th1) polarization.
31214162	1	98	theme	T	420:420	arg1	helper					422:427	type 1 T helper	413:427	type 1 T helper (Th1) polarization	413:446	Mollusk hemocyanins have biomedical uses as carriers/adjuvants and nonspecific immunostimulants with beneficial clinical outcomes by triggering the production of proinflammatory cytokines in antigen-presenting cells (APCs) and driving immune responses toward type 1 T helper (Th1) polarization.
31214162	6	99	theme	dendritic	1086:1094	arg1	DCs					1103:1105	DCs	1103:1105	DCs	1103:1105	Thus, we studied the roles of Dectin-1, Dectin-2, and Toll-like receptor 4 (TLR4) in the hemocyanin activation of murine APCs, both in dendritic cells (DCs) and macrophages, using hemocyanins from Megathura crenulata (KLH), Concholepas concholepas (CCH) and Fissurella latimarginata (FLH).
31214162	6	99	theme	dendritic	1086:1094	arg1	cells					1096:1100	dendritic cells	1086:1100	dendritic cells (DCs)	1086:1106	Thus, we studied the roles of Dectin-1, Dectin-2, and Toll-like receptor 4 (TLR4) in the hemocyanin activation of murine APCs, both in dendritic cells (DCs) and macrophages, using hemocyanins from Megathura crenulata (KLH), Concholepas concholepas (CCH) and Fissurella latimarginata (FLH).
31214162	2	100	theme	hemocyanins	484:494	arg1	patterns					539:546	their glycosylation patterns	519:546	their glycosylation patterns	519:546	Significant structural features of hemocyanins as a model antigen are their glycosylation patterns.
31214162	2	100	theme	hemocyanins	484:494	arg1	features					472:479	Significant structural features	449:479	Significant structural features of hemocyanins as a model antigen	449:513	Significant structural features of hemocyanins as a model antigen are their glycosylation patterns.
31214162	1	101	theme	cytokines	332:340	arg1	production					302:311	the production	298:311	the production of proinflammatory cytokines	298:340	Mollusk hemocyanins have biomedical uses as carriers/adjuvants and nonspecific immunostimulants with beneficial clinical outcomes by triggering the production of proinflammatory cytokines in antigen-presenting cells (APCs) and driving immune responses toward type 1 T helper (Th1) polarization.
31214162	1	102	with	immunostimulants	233:248	arg1	outcomes					275:282	beneficial clinical outcomes	255:282	beneficial clinical outcomes	255:282	Mollusk hemocyanins have biomedical uses as carriers/adjuvants and nonspecific immunostimulants with beneficial clinical outcomes by triggering the production of proinflammatory cytokines in antigen-presenting cells (APCs) and driving immune responses toward type 1 T helper (Th1) polarization.
31214162	7	103	theme	chimeric	1292:1299	arg1	receptors					1323:1331	chimeric Dectin-1 and Dectin-2 receptors	1292:1331	chimeric Dectin-1 and Dectin-2 receptors	1292:1331	The results showed that these hemocyanins bound to chimeric Dectin-1 and Dectin-2 receptors in vitro; which significantly decreased when the glycoproteins were deglycosylated.
31214162	2	104	theme	Significant	449:459	arg1	patterns					539:546	their glycosylation patterns	519:546	their glycosylation patterns	519:546	Significant structural features of hemocyanins as a model antigen are their glycosylation patterns.
31214162	2	104	theme	Significant	449:459	arg1	features					472:479	Significant structural features	449:479	Significant structural features of hemocyanins as a model antigen	449:513	Significant structural features of hemocyanins as a model antigen are their glycosylation patterns.
31214162	1	105	theme	antigen-presenting	345:362	arg1	APCs					371:374	APCs	371:374	APCs	371:374	Mollusk hemocyanins have biomedical uses as carriers/adjuvants and nonspecific immunostimulants with beneficial clinical outcomes by triggering the production of proinflammatory cytokines in antigen-presenting cells (APCs) and driving immune responses toward type 1 T helper (Th1) polarization.
31214162	1	105	theme	antigen-presenting	345:362	arg1	cells					364:368	antigen-presenting cells	345:368	antigen-presenting cells (APCs)	345:375	Mollusk hemocyanins have biomedical uses as carriers/adjuvants and nonspecific immunostimulants with beneficial clinical outcomes by triggering the production of proinflammatory cytokines in antigen-presenting cells (APCs) and driving immune responses toward type 1 T helper (Th1) polarization.
31214162	6	106	theme	APCs	1072:1075	arg1	activation					1051:1060	the hemocyanin activation	1036:1060	the hemocyanin activation of murine APCs, both in dendritic cells (DCs) and macrophages	1036:1122	Thus, we studied the roles of Dectin-1, Dectin-2, and Toll-like receptor 4 (TLR4) in the hemocyanin activation of murine APCs, both in dendritic cells (DCs) and macrophages, using hemocyanins from Megathura crenulata (KLH), Concholepas concholepas (CCH) and Fissurella latimarginata (FLH).
31214162	15	107	from	response	2541:2548	arg1	APCs					2553:2556	APCs	2553:2556	APCs	2553:2556	Our data showed the involvement of TLR4 in the hemocyanin-mediated proinflammatory response in APCs, which could cooperate with MR in innate immune recognition of these glycoproteins.
31214162	5	108	theme	other	843:847	arg1	receptors					863:871	other innate immune receptors	843:871	other innate immune receptors	843:871	However, the contribution of other innate immune receptors to the proinflammatory signaling pathway triggered by hemocyanins is unknown.
31214162	15	109	gly	glycoproteins	2627:2639	arg1	glycoproteins					2627:2639	these glycoproteins	2621:2639	these glycoproteins	2621:2639	Our data showed the involvement of TLR4 in the hemocyanin-mediated proinflammatory response in APCs, which could cooperate with MR in innate immune recognition of these glycoproteins.
31214162	14	110	theme	deficient	2438:2446	arg1	macrophages					2426:2436	macrophages	2426:2436	macrophages deficient for TLR4	2426:2455	Moreover, IL-6 secretion induced by FLH was abolished in macrophages deficient for TLR4.
31214162	0	111	from	Mammals	145:151	arg1	Cells					134:138	Antigen-Presenting Cells	115:138	Antigen-Presenting Cells From Mammals	115:151	TLR4, but Neither Dectin-1 nor Dectin-2, Participates in the Mollusk Hemocyanin-Induced Proinflammatory Effects in Antigen-Presenting Cells From Mammals.
31214162	15	112	theme	TLR4	2493:2496	arg1	involvement					2478:2488	the involvement	2474:2488	the involvement of TLR4 in the hemocyanin-mediated proinflammatory response in APCs, which could cooperate with MR in innate immune recognition of these glycoproteins	2474:2639	Our data showed the involvement of TLR4 in the hemocyanin-mediated proinflammatory response in APCs, which could cooperate with MR in innate immune recognition of these glycoproteins.
31214162	1	113	theme	clinical	266:273	arg1	outcomes					275:282	beneficial clinical outcomes	255:282	beneficial clinical outcomes	255:282	Mollusk hemocyanins have biomedical uses as carriers/adjuvants and nonspecific immunostimulants with beneficial clinical outcomes by triggering the production of proinflammatory cytokines in antigen-presenting cells (APCs) and driving immune responses toward type 1 T helper (Th1) polarization.
31214162	6	114	dep	Concholepas	1175:1185	arg1	concholepas					1187:1197	Concholepas concholepas (CCH)	1175:1203	Concholepas concholepas (CCH)	1175:1203	Thus, we studied the roles of Dectin-1, Dectin-2, and Toll-like receptor 4 (TLR4) in the hemocyanin activation of murine APCs, both in dendritic cells (DCs) and macrophages, using hemocyanins from Megathura crenulata (KLH), Concholepas concholepas (CCH) and Fissurella latimarginata (FLH).
31214162	8	115	theme	proinflammatory	1445:1459	arg1	effects					1461:1467	hemocyanin-induced proinflammatory effects	1426:1467	hemocyanin-induced proinflammatory effects in APCs from Dectin-1 knock-out (KO) and Dectin-2 KO mice	1426:1525	However, hemocyanin-induced proinflammatory effects in APCs from Dectin-1 knock-out (KO) and Dectin-2 KO mice were independent of both receptors.
31214162	8	116	from	mice	1522:1525	arg1	APCs					1472:1475	APCs	1472:1475	APCs from Dectin-1 knock-out (KO) and Dectin-2 KO mice	1472:1525	However, hemocyanin-induced proinflammatory effects in APCs from Dectin-1 knock-out (KO) and Dectin-2 KO mice were independent of both receptors.
31214162	8	116	from	mice	1522:1525	arg1	effects					1461:1467	hemocyanin-induced proinflammatory effects	1426:1467	hemocyanin-induced proinflammatory effects in APCs from Dectin-1 knock-out (KO) and Dectin-2 KO mice	1426:1525	However, hemocyanin-induced proinflammatory effects in APCs from Dectin-1 knock-out (KO) and Dectin-2 KO mice were independent of both receptors.
31223543	8	0	theme	radical	1178:1184	arg1	rate					1197:1200	its superoxide anion radical scavenging rate	1157:1200	its superoxide anion radical scavenging rate	1157:1200	The 2,2-diphenyl-1-picrylhydrazyl radical scavenging rate of two mg/mL AHP-H-2 was 80%, its hydroxyl radical scavenging rate was approximately 48.5%, its superoxide anion radical scavenging rate was 85.3% and its reduction ability of Fe3+ was approximately 0.92.
31223543	9	1	theme	milligram	1309:1317	arg1	6.5					1334:1336	6.5	1334:1336	6.5	1334:1336	The total antioxidant capacity of each milligram of AHP-H-2 was 6.5, which was higher than ascorbic acid.
31223543	9	1	theme	milligram	1309:1317	arg1	capacity					1292:1299	The total antioxidant capacity	1270:1299	The total antioxidant capacity of each milligram of AHP-H-2	1270:1328	The total antioxidant capacity of each milligram of AHP-H-2 was 6.5, which was higher than ascorbic acid.
31223543	8	2	theme	AHP-H-2	1078:1084	arg1	rate					1060:1063	The 2,2-diphenyl-1-picrylhydrazyl radical scavenging rate	1007:1063	The 2,2-diphenyl-1-picrylhydrazyl radical scavenging rate of two mg/mL AHP-H-2	1007:1084	The 2,2-diphenyl-1-picrylhydrazyl radical scavenging rate of two mg/mL AHP-H-2 was 80%, its hydroxyl radical scavenging rate was approximately 48.5%, its superoxide anion radical scavenging rate was 85.3% and its reduction ability of Fe3+ was approximately 0.92.
31223543	8	2	theme	AHP-H-2	1078:1084	arg1	%					1092:1092	80%	1090:1092	80%	1090:1092	The 2,2-diphenyl-1-picrylhydrazyl radical scavenging rate of two mg/mL AHP-H-2 was 80%, its hydroxyl radical scavenging rate was approximately 48.5%, its superoxide anion radical scavenging rate was 85.3% and its reduction ability of Fe3+ was approximately 0.92.
31223543	2	3	from	hybridus	389:396	arg1	polysaccharides					365:379	polysaccharides	365:379	polysaccharides from A. hybridus	365:396	However, the composition of polysaccharides from A. hybridus has rarely been previously reported.
31223543	2	3	from	hybridus	389:396	arg1	composition					350:360	the composition	346:360	the composition of polysaccharides from A. hybridus	346:396	However, the composition of polysaccharides from A. hybridus has rarely been previously reported.
31223543	4	4	theme	diethylaminoethyl	664:680	arg1	chromatography					702:715	diethylaminoethyl cellulose-32 column chromatography	664:715	diethylaminoethyl cellulose-32 column chromatography	664:715	Two acidic polysaccharides were isolated and purified by the Sevage method and diethylaminoethyl cellulose-32 column chromatography.
31223543	1	5	theme	grain	232:236	arg1	family					200:205	the Amaranthceae family	183:205	the Amaranthceae family	183:205	BACKGROUND Amaranthus hybridus L. is an annual herb that belongs to the Amaranthceae family, a type of multi-purpose grain, vegetable and feed crop that has received considerable attention due to its great economic value.
31223543	1	5	theme	grain	232:236	arg1	vegetable					239:247	vegetable	239:247	vegetable	239:247	BACKGROUND Amaranthus hybridus L. is an annual herb that belongs to the Amaranthceae family, a type of multi-purpose grain, vegetable and feed crop that has received considerable attention due to its great economic value.
31223543	1	5	theme	grain	232:236	arg1	type					210:213	a type	208:213	a type of multi-purpose grain	208:236	BACKGROUND Amaranthus hybridus L. is an annual herb that belongs to the Amaranthceae family, a type of multi-purpose grain, vegetable and feed crop that has received considerable attention due to its great economic value.
31223543	1	5	theme	grain	232:236	arg1	crop					258:261	feed crop	253:261	feed crop	253:261	BACKGROUND Amaranthus hybridus L. is an annual herb that belongs to the Amaranthceae family, a type of multi-purpose grain, vegetable and feed crop that has received considerable attention due to its great economic value.
31223543	8	6	theme	anion	1172:1176	arg1	radical					1178:1184	its superoxide anion radical	1157:1184	its superoxide anion radical scavenging rate	1157:1200	The 2,2-diphenyl-1-picrylhydrazyl radical scavenging rate of two mg/mL AHP-H-2 was 80%, its hydroxyl radical scavenging rate was approximately 48.5%, its superoxide anion radical scavenging rate was 85.3% and its reduction ability of Fe3+ was approximately 0.92.
31223543	1	7	theme	great	315:319	arg1	value					330:334	its great economic value	311:334	its great economic value	311:334	BACKGROUND Amaranthus hybridus L. is an annual herb that belongs to the Amaranthceae family, a type of multi-purpose grain, vegetable and feed crop that has received considerable attention due to its great economic value.
31223543	8	8	theme	mg/mL	1072:1076	arg1	AHP-H-2					1078:1084	two mg/mL AHP-H-2	1068:1084	two mg/mL AHP-H-2	1068:1084	The 2,2-diphenyl-1-picrylhydrazyl radical scavenging rate of two mg/mL AHP-H-2 was 80%, its hydroxyl radical scavenging rate was approximately 48.5%, its superoxide anion radical scavenging rate was 85.3% and its reduction ability of Fe3+ was approximately 0.92.
31223543	8	9	theme	Fe3+	1241:1244	arg1	ability					1230:1236	its reduction ability	1216:1236	its reduction ability of Fe3+	1216:1244	The 2,2-diphenyl-1-picrylhydrazyl radical scavenging rate of two mg/mL AHP-H-2 was 80%, its hydroxyl radical scavenging rate was approximately 48.5%, its superoxide anion radical scavenging rate was 85.3% and its reduction ability of Fe3+ was approximately 0.92.
31223543	1	10	theme	annual	155:160	arg1	herb					162:165	an annual herb	152:165	an annual herb that belongs to the Amaranthceae family, a type of multi-purpose grain, vegetable and feed crop that has received considerable attention due to its great economic value	152:334	BACKGROUND Amaranthus hybridus L. is an annual herb that belongs to the Amaranthceae family, a type of multi-purpose grain, vegetable and feed crop that has received considerable attention due to its great economic value.
31223543	1	10	theme	annual	155:160	arg1	BACKGROUND					115:124	BACKGROUND	115:124	BACKGROUND Amaranthus hybridus L.	115:147	BACKGROUND Amaranthus hybridus L. is an annual herb that belongs to the Amaranthceae family, a type of multi-purpose grain, vegetable and feed crop that has received considerable attention due to its great economic value.
31223543	1	11	theme	economic	321:328	arg1	value					330:334	its great economic value	311:334	its great economic value	311:334	BACKGROUND Amaranthus hybridus L. is an annual herb that belongs to the Amaranthceae family, a type of multi-purpose grain, vegetable and feed crop that has received considerable attention due to its great economic value.
31223543	9	12	theme	AHP-H-2	1322:1328	arg1	milligram					1309:1317	each milligram	1304:1317	each milligram of AHP-H-2	1304:1328	The total antioxidant capacity of each milligram of AHP-H-2 was 6.5, which was higher than ascorbic acid.
31223543	6	13	from	sample	886:891	arg1	content					868:874	the monosaccharide content	849:874	the monosaccharide content from each sample according to gas chromatography-mass spectrometer	849:941	There were significant differences between the monosaccharide content from each sample according to gas chromatography-mass spectrometer.
31223543	6	14	theme	gas	906:908	arg1	spectrometer					930:941	gas chromatography-mass spectrometer	906:941	gas chromatography-mass spectrometer	906:941	There were significant differences between the monosaccharide content from each sample according to gas chromatography-mass spectrometer.
31223543	8	15	theme	radical	1041:1047	arg1	rate					1060:1063	The 2,2-diphenyl-1-picrylhydrazyl radical scavenging rate	1007:1063	The 2,2-diphenyl-1-picrylhydrazyl radical scavenging rate of two mg/mL AHP-H-2	1007:1084	The 2,2-diphenyl-1-picrylhydrazyl radical scavenging rate of two mg/mL AHP-H-2 was 80%, its hydroxyl radical scavenging rate was approximately 48.5%, its superoxide anion radical scavenging rate was 85.3% and its reduction ability of Fe3+ was approximately 0.92.
31223543	8	15	theme	radical	1041:1047	arg1	%					1092:1092	80%	1090:1092	80%	1090:1092	The 2,2-diphenyl-1-picrylhydrazyl radical scavenging rate of two mg/mL AHP-H-2 was 80%, its hydroxyl radical scavenging rate was approximately 48.5%, its superoxide anion radical scavenging rate was 85.3% and its reduction ability of Fe3+ was approximately 0.92.
31223543	0	16	theme	activity	105:112	arg1	Extraction					0:9	Extraction	0:9	Extraction of polysaccharides from Amaranthus hybridus L. by hot water	0:69	Extraction of polysaccharides from Amaranthus hybridus L. by hot water and analysis of their antioxidant activity.
31223543	0	16	theme	activity	105:112	arg1	analysis					75:82	analysis	75:82	analysis of their antioxidant activity	75:112	Extraction of polysaccharides from Amaranthus hybridus L. by hot water and analysis of their antioxidant activity.
31223543	0	17	from	L.	55:56	arg1	polysaccharides					14:28	polysaccharides	14:28	polysaccharides from Amaranthus hybridus L.	14:56	Extraction of polysaccharides from Amaranthus hybridus L. by hot water and analysis of their antioxidant activity.
31223543	0	17	from	L.	55:56	arg1	Extraction					0:9	Extraction	0:9	Extraction of polysaccharides from Amaranthus hybridus L. by hot water	0:69	Extraction of polysaccharides from Amaranthus hybridus L. by hot water and analysis of their antioxidant activity.
31223543	0	17	from	L.	55:56	arg1	analysis					75:82	analysis	75:82	analysis of their antioxidant activity	75:112	Extraction of polysaccharides from Amaranthus hybridus L. by hot water and analysis of their antioxidant activity.
31223543	4	18	theme	acidic	589:594	arg1	polysaccharides					596:610	Two acidic polysaccharides	585:610	Two acidic polysaccharides	585:610	Two acidic polysaccharides were isolated and purified by the Sevage method and diethylaminoethyl cellulose-32 column chromatography.
31223543	3	19	used	used	498:501	arg2	polysaccharides					519:533	polysaccharides	519:533	polysaccharides	519:533	METHODS In this study, the aboveground part of A. hybridus was used as material and polysaccharides were isolated by the hot water extraction method.
31223543	3	19	used	used	498:501	arg2	material					506:513	material	506:513	material	506:513	METHODS In this study, the aboveground part of A. hybridus was used as material and polysaccharides were isolated by the hot water extraction method.
31223543	3	19	used	used	498:501	arg2	part					474:477	the aboveground part	458:477	the aboveground part of A. hybridus	458:492	METHODS In this study, the aboveground part of A. hybridus was used as material and polysaccharides were isolated by the hot water extraction method.
31223543	0	20	theme	antioxidant	93:103	arg1	activity					105:112	their antioxidant activity	87:112	their antioxidant activity	87:112	Extraction of polysaccharides from Amaranthus hybridus L. by hot water and analysis of their antioxidant activity.
31223543	10	21	theme	theoretical	1467:1477	arg1	basis					1479:1483	a theoretical basis	1465:1483	a theoretical basis for its development	1465:1503	CONCLUSION The results of the study promote the effective use of A. hybridus and provide a theoretical basis for its development.
31223543	7	22	theme	antioxidant	963:973	arg1	activity					975:982	higher antioxidant activity	956:982	higher antioxidant activity	956:982	AHP-H-2 had higher antioxidant activity in vitro than AHP-H-1.
31223543	5	23	theme	acidic	730:735	arg1	polysaccharides					737:751	Two acidic polysaccharides	726:751	Two acidic polysaccharides	726:751	RESULTS Two acidic polysaccharides were obtained from A. hybridus: AHP-H-1 and AHP-H-2.
31223543	9	24	theme	ascorbic	1361:1368	arg1	acid					1370:1373	ascorbic acid	1361:1373	ascorbic acid	1361:1373	The total antioxidant capacity of each milligram of AHP-H-2 was 6.5, which was higher than ascorbic acid.
31223543	8	25	theme	hydroxyl	1099:1106	arg1	radical					1108:1114	its hydroxyl radical	1095:1114	its hydroxyl radical scavenging rate	1095:1130	The 2,2-diphenyl-1-picrylhydrazyl radical scavenging rate of two mg/mL AHP-H-2 was 80%, its hydroxyl radical scavenging rate was approximately 48.5%, its superoxide anion radical scavenging rate was 85.3% and its reduction ability of Fe3+ was approximately 0.92.
31223543	8	26	theme	scavenging	1049:1058	arg1	rate					1060:1063	The 2,2-diphenyl-1-picrylhydrazyl radical scavenging rate	1007:1063	The 2,2-diphenyl-1-picrylhydrazyl radical scavenging rate of two mg/mL AHP-H-2	1007:1084	The 2,2-diphenyl-1-picrylhydrazyl radical scavenging rate of two mg/mL AHP-H-2 was 80%, its hydroxyl radical scavenging rate was approximately 48.5%, its superoxide anion radical scavenging rate was 85.3% and its reduction ability of Fe3+ was approximately 0.92.
31223543	8	26	theme	scavenging	1049:1058	arg1	%					1092:1092	80%	1090:1092	80%	1090:1092	The 2,2-diphenyl-1-picrylhydrazyl radical scavenging rate of two mg/mL AHP-H-2 was 80%, its hydroxyl radical scavenging rate was approximately 48.5%, its superoxide anion radical scavenging rate was 85.3% and its reduction ability of Fe3+ was approximately 0.92.
31223543	10	27	dep	CONCLUSION	1376:1385	arg1	provide					1457:1463	provide	1457:1463	provide a theoretical basis for its development	1457:1503	CONCLUSION The results of the study promote the effective use of A. hybridus and provide a theoretical basis for its development.
31223543	10	27	dep	CONCLUSION	1376:1385	arg1	promote					1412:1418	promote	1412:1418	promote the effective use of A. hybridus	1412:1451	CONCLUSION The results of the study promote the effective use of A. hybridus and provide a theoretical basis for its development.
31223543	7	28	contain	had	952:954	arg2	activity					975:982	higher antioxidant activity	956:982	higher antioxidant activity	956:982	AHP-H-2 had higher antioxidant activity in vitro than AHP-H-1.
31223543	7	28	contain	had	952:954	arg1	AHP-H-2					944:950	AHP-H-2	944:950	AHP-H-2	944:950	AHP-H-2 had higher antioxidant activity in vitro than AHP-H-1.
31223543	1	29	theme	feed	253:256	arg1	family					200:205	the Amaranthceae family	183:205	the Amaranthceae family	183:205	BACKGROUND Amaranthus hybridus L. is an annual herb that belongs to the Amaranthceae family, a type of multi-purpose grain, vegetable and feed crop that has received considerable attention due to its great economic value.
31223543	1	29	theme	feed	253:256	arg1	crop					258:261	feed crop	253:261	feed crop	253:261	BACKGROUND Amaranthus hybridus L. is an annual herb that belongs to the Amaranthceae family, a type of multi-purpose grain, vegetable and feed crop that has received considerable attention due to its great economic value.
31223543	10	30	theme	hybridus	1444:1451	arg1	use					1434:1436	the effective use	1420:1436	the effective use of A. hybridus	1420:1451	CONCLUSION The results of the study promote the effective use of A. hybridus and provide a theoretical basis for its development.
31223543	8	31	theme	reduction	1220:1228	arg1	ability					1230:1236	its reduction ability	1216:1236	its reduction ability of Fe3+	1216:1244	The 2,2-diphenyl-1-picrylhydrazyl radical scavenging rate of two mg/mL AHP-H-2 was 80%, its hydroxyl radical scavenging rate was approximately 48.5%, its superoxide anion radical scavenging rate was 85.3% and its reduction ability of Fe3+ was approximately 0.92.
31223543	8	32	theme	scavenging	1116:1125	arg1	rate					1127:1130	its hydroxyl radical scavenging rate	1095:1130	its hydroxyl radical scavenging rate	1095:1130	The 2,2-diphenyl-1-picrylhydrazyl radical scavenging rate of two mg/mL AHP-H-2 was 80%, its hydroxyl radical scavenging rate was approximately 48.5%, its superoxide anion radical scavenging rate was 85.3% and its reduction ability of Fe3+ was approximately 0.92.
31223543	7	33	theme	higher	956:961	arg1	activity					975:982	higher antioxidant activity	956:982	higher antioxidant activity	956:982	AHP-H-2 had higher antioxidant activity in vitro than AHP-H-1.
31223543	10	34	theme	study	1406:1410	arg1	results					1391:1397	The results	1387:1397	The results of the study	1387:1410	CONCLUSION The results of the study promote the effective use of A. hybridus and provide a theoretical basis for its development.
31223543	0	35	theme	polysaccharides	14:28	arg1	Extraction					0:9	Extraction	0:9	Extraction of polysaccharides from Amaranthus hybridus L. by hot water	0:69	Extraction of polysaccharides from Amaranthus hybridus L. by hot water and analysis of their antioxidant activity.
31223543	0	35	theme	polysaccharides	14:28	arg1	analysis					75:82	analysis	75:82	analysis of their antioxidant activity	75:112	Extraction of polysaccharides from Amaranthus hybridus L. by hot water and analysis of their antioxidant activity.
31223543	8	36	theme	radical	1108:1114	arg1	rate					1127:1130	its hydroxyl radical scavenging rate	1095:1130	its hydroxyl radical scavenging rate	1095:1130	The 2,2-diphenyl-1-picrylhydrazyl radical scavenging rate of two mg/mL AHP-H-2 was 80%, its hydroxyl radical scavenging rate was approximately 48.5%, its superoxide anion radical scavenging rate was 85.3% and its reduction ability of Fe3+ was approximately 0.92.
31223543	0	37	from	analysis	75:82	arg1	L.					55:56	Amaranthus hybridus L.	35:56	Amaranthus hybridus L.	35:56	Extraction of polysaccharides from Amaranthus hybridus L. by hot water and analysis of their antioxidant activity.
31223543	1	38	theme	Amaranthceae	187:198	arg1	vegetable					239:247	vegetable	239:247	vegetable	239:247	BACKGROUND Amaranthus hybridus L. is an annual herb that belongs to the Amaranthceae family, a type of multi-purpose grain, vegetable and feed crop that has received considerable attention due to its great economic value.
31223543	1	38	theme	Amaranthceae	187:198	arg1	family					200:205	the Amaranthceae family	183:205	the Amaranthceae family	183:205	BACKGROUND Amaranthus hybridus L. is an annual herb that belongs to the Amaranthceae family, a type of multi-purpose grain, vegetable and feed crop that has received considerable attention due to its great economic value.
31223543	1	38	theme	Amaranthceae	187:198	arg1	type					210:213	a type	208:213	a type of multi-purpose grain	208:236	BACKGROUND Amaranthus hybridus L. is an annual herb that belongs to the Amaranthceae family, a type of multi-purpose grain, vegetable and feed crop that has received considerable attention due to its great economic value.
31223543	1	38	theme	Amaranthceae	187:198	arg1	crop					258:261	feed crop	253:261	feed crop	253:261	BACKGROUND Amaranthus hybridus L. is an annual herb that belongs to the Amaranthceae family, a type of multi-purpose grain, vegetable and feed crop that has received considerable attention due to its great economic value.
31223543	4	39	theme	cellulose-32	682:693	arg1	chromatography					702:715	diethylaminoethyl cellulose-32 column chromatography	664:715	diethylaminoethyl cellulose-32 column chromatography	664:715	Two acidic polysaccharides were isolated and purified by the Sevage method and diethylaminoethyl cellulose-32 column chromatography.
31223543	3	40	theme	hot	556:558	arg1	method					577:582	the hot water extraction method	552:582	the hot water extraction method	552:582	METHODS In this study, the aboveground part of A. hybridus was used as material and polysaccharides were isolated by the hot water extraction method.
31223543	2	41	theme	polysaccharides	365:379	arg1	composition					350:360	the composition	346:360	the composition of polysaccharides from A. hybridus	346:396	However, the composition of polysaccharides from A. hybridus has rarely been previously reported.
31223543	3	42	theme	water	560:564	arg1	method					577:582	the hot water extraction method	552:582	the hot water extraction method	552:582	METHODS In this study, the aboveground part of A. hybridus was used as material and polysaccharides were isolated by the hot water extraction method.
31223543	2	43	from	composition	350:360	arg1	hybridus					389:396	A. hybridus	386:396	A. hybridus	386:396	However, the composition of polysaccharides from A. hybridus has rarely been previously reported.
31223543	10	44	theme	effective	1424:1432	arg1	use					1434:1436	the effective use	1420:1436	the effective use of A. hybridus	1420:1451	CONCLUSION The results of the study promote the effective use of A. hybridus and provide a theoretical basis for its development.
31223543	1	45	theme	considerable	281:292	arg1	attention					294:302	considerable attention	281:302	considerable attention due to its great economic value	281:334	BACKGROUND Amaranthus hybridus L. is an annual herb that belongs to the Amaranthceae family, a type of multi-purpose grain, vegetable and feed crop that has received considerable attention due to its great economic value.
31223543	6	46	theme	monosaccharide	853:866	arg1	content					868:874	the monosaccharide content	849:874	the monosaccharide content from each sample according to gas chromatography-mass spectrometer	849:941	There were significant differences between the monosaccharide content from each sample according to gas chromatography-mass spectrometer.
31223543	4	47	theme	column	695:700	arg1	chromatography					702:715	diethylaminoethyl cellulose-32 column chromatography	664:715	diethylaminoethyl cellulose-32 column chromatography	664:715	Two acidic polysaccharides were isolated and purified by the Sevage method and diethylaminoethyl cellulose-32 column chromatography.
31223543	0	48	from	Extraction	0:9	arg1	L.					55:56	Amaranthus hybridus L.	35:56	Amaranthus hybridus L.	35:56	Extraction of polysaccharides from Amaranthus hybridus L. by hot water and analysis of their antioxidant activity.
31223543	9	49	theme	total	1274:1278	arg1	6.5					1334:1336	6.5	1334:1336	6.5	1334:1336	The total antioxidant capacity of each milligram of AHP-H-2 was 6.5, which was higher than ascorbic acid.
31223543	9	49	theme	total	1274:1278	arg1	capacity					1292:1299	The total antioxidant capacity	1270:1299	The total antioxidant capacity of each milligram of AHP-H-2	1270:1328	The total antioxidant capacity of each milligram of AHP-H-2 was 6.5, which was higher than ascorbic acid.
31223543	9	50	theme	antioxidant	1280:1290	arg1	6.5					1334:1336	6.5	1334:1336	6.5	1334:1336	The total antioxidant capacity of each milligram of AHP-H-2 was 6.5, which was higher than ascorbic acid.
31223543	9	50	theme	antioxidant	1280:1290	arg1	capacity					1292:1299	The total antioxidant capacity	1270:1299	The total antioxidant capacity of each milligram of AHP-H-2	1270:1328	The total antioxidant capacity of each milligram of AHP-H-2 was 6.5, which was higher than ascorbic acid.
31223543	3	51	theme	aboveground	462:472	arg1	polysaccharides					519:533	polysaccharides	519:533	polysaccharides	519:533	METHODS In this study, the aboveground part of A. hybridus was used as material and polysaccharides were isolated by the hot water extraction method.
31223543	3	51	theme	aboveground	462:472	arg1	material					506:513	material	506:513	material	506:513	METHODS In this study, the aboveground part of A. hybridus was used as material and polysaccharides were isolated by the hot water extraction method.
31223543	3	51	theme	aboveground	462:472	arg1	part					474:477	the aboveground part	458:477	the aboveground part of A. hybridus	458:492	METHODS In this study, the aboveground part of A. hybridus was used as material and polysaccharides were isolated by the hot water extraction method.
31223543	1	52	theme	due	304:306	arg1	attention					294:302	considerable attention	281:302	considerable attention due to its great economic value	281:334	BACKGROUND Amaranthus hybridus L. is an annual herb that belongs to the Amaranthceae family, a type of multi-purpose grain, vegetable and feed crop that has received considerable attention due to its great economic value.
31223543	5	53	dep	RESULTS	718:724	arg1	AHP-H-1					785:791	AHP-H-1	785:791	AHP-H-1	785:791	RESULTS Two acidic polysaccharides were obtained from A. hybridus: AHP-H-1 and AHP-H-2.
31223543	5	53	dep	RESULTS	718:724	arg1	obtained					758:765	obtained	758:765	were obtained from A. hybridus	753:782	RESULTS Two acidic polysaccharides were obtained from A. hybridus: AHP-H-1 and AHP-H-2.
31223543	5	53	dep	RESULTS	718:724	arg1	RESULTS					718:724	RESULTS	718:724	RESULTS Two acidic polysaccharides were obtained from A. hybridus: AHP-H-1 and AHP-H-2.	718:804	RESULTS Two acidic polysaccharides were obtained from A. hybridus: AHP-H-1 and AHP-H-2.
31223543	5	53	dep	RESULTS	718:724	arg1	AHP-H-2					797:803	AHP-H-2	797:803	AHP-H-2	797:803	RESULTS Two acidic polysaccharides were obtained from A. hybridus: AHP-H-1 and AHP-H-2.
31223543	8	54	theme	scavenging	1186:1195	arg1	rate					1197:1200	its superoxide anion radical scavenging rate	1157:1200	its superoxide anion radical scavenging rate	1157:1200	The 2,2-diphenyl-1-picrylhydrazyl radical scavenging rate of two mg/mL AHP-H-2 was 80%, its hydroxyl radical scavenging rate was approximately 48.5%, its superoxide anion radical scavenging rate was 85.3% and its reduction ability of Fe3+ was approximately 0.92.
31223543	8	55	theme	2,2-diphenyl-1-picrylhydrazyl	1011:1039	arg1	rate					1060:1063	The 2,2-diphenyl-1-picrylhydrazyl radical scavenging rate	1007:1063	The 2,2-diphenyl-1-picrylhydrazyl radical scavenging rate of two mg/mL AHP-H-2	1007:1084	The 2,2-diphenyl-1-picrylhydrazyl radical scavenging rate of two mg/mL AHP-H-2 was 80%, its hydroxyl radical scavenging rate was approximately 48.5%, its superoxide anion radical scavenging rate was 85.3% and its reduction ability of Fe3+ was approximately 0.92.
31223543	8	55	theme	2,2-diphenyl-1-picrylhydrazyl	1011:1039	arg1	%					1092:1092	80%	1090:1092	80%	1090:1092	The 2,2-diphenyl-1-picrylhydrazyl radical scavenging rate of two mg/mL AHP-H-2 was 80%, its hydroxyl radical scavenging rate was approximately 48.5%, its superoxide anion radical scavenging rate was 85.3% and its reduction ability of Fe3+ was approximately 0.92.
31223543	3	56	dep	METHODS	435:441	arg1	used					498:501	used	498:501	was used as material and polysaccharides	494:533	METHODS In this study, the aboveground part of A. hybridus was used as material and polysaccharides were isolated by the hot water extraction method.
31223543	4	57	theme	Sevage	646:651	arg1	method					653:658	the Sevage method	642:658	the Sevage method	642:658	Two acidic polysaccharides were isolated and purified by the Sevage method and diethylaminoethyl cellulose-32 column chromatography.
31223543	8	58	theme	superoxide	1161:1170	arg1	radical					1178:1184	its superoxide anion radical	1157:1184	its superoxide anion radical scavenging rate	1157:1200	The 2,2-diphenyl-1-picrylhydrazyl radical scavenging rate of two mg/mL AHP-H-2 was 80%, its hydroxyl radical scavenging rate was approximately 48.5%, its superoxide anion radical scavenging rate was 85.3% and its reduction ability of Fe3+ was approximately 0.92.
31223543	6	59	theme	significant	817:827	arg1	differences					829:839	significant differences	817:839	significant differences between the monosaccharide content from each sample according to gas chromatography-mass spectrometer	817:941	There were significant differences between the monosaccharide content from each sample according to gas chromatography-mass spectrometer.
31223543	3	60	theme	extraction	566:575	arg1	method					577:582	the hot water extraction method	552:582	the hot water extraction method	552:582	METHODS In this study, the aboveground part of A. hybridus was used as material and polysaccharides were isolated by the hot water extraction method.
31223543	6	61	theme	chromatography-mass	910:928	arg1	spectrometer					930:941	gas chromatography-mass spectrometer	906:941	gas chromatography-mass spectrometer	906:941	There were significant differences between the monosaccharide content from each sample according to gas chromatography-mass spectrometer.
31223543	0	62	theme	hot	61:63	arg1	water					65:69	hot water	61:69	hot water	61:69	Extraction of polysaccharides from Amaranthus hybridus L. by hot water and analysis of their antioxidant activity.
31223543	3	63	theme	hybridus	485:492	arg1	polysaccharides					519:533	polysaccharides	519:533	polysaccharides	519:533	METHODS In this study, the aboveground part of A. hybridus was used as material and polysaccharides were isolated by the hot water extraction method.
31223543	3	63	theme	hybridus	485:492	arg1	material					506:513	material	506:513	material	506:513	METHODS In this study, the aboveground part of A. hybridus was used as material and polysaccharides were isolated by the hot water extraction method.
31223543	3	63	theme	hybridus	485:492	arg1	part					474:477	the aboveground part	458:477	the aboveground part of A. hybridus	458:492	METHODS In this study, the aboveground part of A. hybridus was used as material and polysaccharides were isolated by the hot water extraction method.
31223543	1	64	theme	multi-purpose	218:230	arg1	grain					232:236	multi-purpose grain	218:236	multi-purpose grain	218:236	BACKGROUND Amaranthus hybridus L. is an annual herb that belongs to the Amaranthceae family, a type of multi-purpose grain, vegetable and feed crop that has received considerable attention due to its great economic value.
29957342	9	0	theme	glycan	1606:1611	arg1	degradation					1613:1623	glycan degradation	1606:1623	glycan degradation	1606:1623	Chlorosis rank was moderately correlated with KEGG Orthology (KO) terms associated with nitrogen (N) and carbon (C) metabolism in soils, while leaf Mg was associated with a spectrum of KO terms including glycosphingolipid biosynthesis, glycan degradation, transporters, and porphyrin and chlorophyll metabolism.
29957342	9	1	theme	Orthology	1421:1429	arg1	terms					1436:1440	KEGG Orthology (KO) terms	1416:1440	KEGG Orthology (KO) terms associated with nitrogen (N) and carbon (C) metabolism in soils	1416:1504	Chlorosis rank was moderately correlated with KEGG Orthology (KO) terms associated with nitrogen (N) and carbon (C) metabolism in soils, while leaf Mg was associated with a spectrum of KO terms including glycosphingolipid biosynthesis, glycan degradation, transporters, and porphyrin and chlorophyll metabolism.
29957342	8	2	theme	Non-metric	1194:1203	arg1	NMDS					1231:1234	NMDS	1231:1234	NMDS	1231:1234	Non-metric multidimensional scaling (NMDS) revealed a significant correlation between fungal community composition and soil Fe and pH, along with leaf N, Mg, and Ca (mg.kg-1).
29957342	8	2	theme	Non-metric	1194:1203	arg1	scaling					1222:1228	Non-metric multidimensional scaling	1194:1228	Non-metric multidimensional scaling (NMDS)	1194:1235	Non-metric multidimensional scaling (NMDS) revealed a significant correlation between fungal community composition and soil Fe and pH, along with leaf N, Mg, and Ca (mg.kg-1).
29957342	6	3	theme	bacterial	886:894	arg1	composition					906:916	bacterial community composition	886:916	bacterial community composition	886:916	Finally, factors influencing bacterial community composition were correlated with predicted bacterial community function.
29957342	4	4	used	used	508:511	arg2	We					505:506	We	505:506	We	505:506	We used next-generation sequencing (NGS) to examine the bacterial and fungal communities associated with grapevines demonstrating varying degrees of visual chlorosis symptoms.
29957342	10	5	theme	taxonomic	1736:1744	arg1	units					1746:1750	many bacterial operational taxonomic units	1709:1750	many bacterial operational taxonomic units	1709:1750	Additionally, abundance of many bacterial operational taxonomic units was significantly correlated with leaf Mg, including those from the following orders: Rhodobacterales, Acidobacteriales, Opitutales, Sphingomonadales, Burkholderiales, Saprospirales, and Flavobacteriales.
29957342	9	6	theme	KO	1432:1433	arg1	terms					1436:1440	KEGG Orthology (KO) terms	1416:1440	KEGG Orthology (KO) terms associated with nitrogen (N) and carbon (C) metabolism in soils	1416:1504	Chlorosis rank was moderately correlated with KEGG Orthology (KO) terms associated with nitrogen (N) and carbon (C) metabolism in soils, while leaf Mg was associated with a spectrum of KO terms including glycosphingolipid biosynthesis, glycan degradation, transporters, and porphyrin and chlorophyll metabolism.
29957342	9	7	from	metabolism	1486:1495	arg1	soils					1500:1504	soils	1500:1504	soils	1500:1504	Chlorosis rank was moderately correlated with KEGG Orthology (KO) terms associated with nitrogen (N) and carbon (C) metabolism in soils, while leaf Mg was associated with a spectrum of KO terms including glycosphingolipid biosynthesis, glycan degradation, transporters, and porphyrin and chlorophyll metabolism.
29957342	8	8	theme	community	1287:1295	arg1	composition					1297:1307	fungal community composition	1280:1307	fungal community composition	1280:1307	Non-metric multidimensional scaling (NMDS) revealed a significant correlation between fungal community composition and soil Fe and pH, along with leaf N, Mg, and Ca (mg.kg-1).
29957342	3	9	theme	little	487:492	arg1	attention					494:502	little attention	487:502	little attention	487:502	Physiology of chlorosis in vineyards has been well-studied, but the soil microbial consequences of and contributions to chlorosis have received little attention.
29957342	1	10	theme	reduced	165:171	arg1	yields					178:183	reduced crop yields	165:183	reduced crop yields	165:183	Leaf chlorosis in vineyards is associated with reduced crop yields and quality.
29957342	9	11	theme	porphyrin	1644:1652	arg1	metabolism					1670:1679	porphyrin and chlorophyll metabolism	1644:1679	metabolism	1670:1679	Chlorosis rank was moderately correlated with KEGG Orthology (KO) terms associated with nitrogen (N) and carbon (C) metabolism in soils, while leaf Mg was associated with a spectrum of KO terms including glycosphingolipid biosynthesis, glycan degradation, transporters, and porphyrin and chlorophyll metabolism.
29957342	2	12	theme	total	265:269	arg1	plant					271:275	total plant	265:275	total plant	265:275	While iron (Fe) is understood to play a crucial role in chlorosis, total plant and soil Fe are not always indicative of chlorosis in grapevines.
29957342	11	13	dep	suggest	1970:1976	arg1	interrelated					2001:2012	interrelated	2001:2012	suggest grapevine chlorosis is interrelated with soil microbial community structure and function, plant nutrition, and soil chemistry	1970:2102	Our findings suggest grapevine chlorosis is interrelated with soil microbial community structure and function, plant nutrition, and soil chemistry.
29957342	9	14	theme	chlorophyll	1658:1668	arg1	metabolism					1670:1679	porphyrin and chlorophyll metabolism	1644:1679	metabolism	1670:1679	Chlorosis rank was moderately correlated with KEGG Orthology (KO) terms associated with nitrogen (N) and carbon (C) metabolism in soils, while leaf Mg was associated with a spectrum of KO terms including glycosphingolipid biosynthesis, glycan degradation, transporters, and porphyrin and chlorophyll metabolism.
29957342	5	15	theme	soil	806:809	arg1	chemistry					811:819	soil chemistry	806:819	soil chemistry	806:819	Additionally, chemical analyses of soils and grape leaves were used to explore the influence of plant nutritional status and soil chemistry on microbial community composition.
29957342	8	16	theme	significant	1248:1258	arg1	correlation					1260:1270	a significant correlation	1246:1270	a significant correlation between fungal community composition and soil Fe and pH	1246:1326	Non-metric multidimensional scaling (NMDS) revealed a significant correlation between fungal community composition and soil Fe and pH, along with leaf N, Mg, and Ca (mg.kg-1).
29957342	6	17	theme	community	959:967	arg1	function					969:976	predicted bacterial community function	939:976	predicted bacterial community function	939:976	Finally, factors influencing bacterial community composition were correlated with predicted bacterial community function.
29957342	5	18	dep	grape	726:730	arg1	leaves					732:737	leaves	732:737	leaves	732:737	Additionally, chemical analyses of soils and grape leaves were used to explore the influence of plant nutritional status and soil chemistry on microbial community composition.
29957342	5	19	theme	chemical	695:702	arg1	analyses					704:711	chemical analyses	695:711	chemical analyses of soils and grape leaves	695:737	Additionally, chemical analyses of soils and grape leaves were used to explore the influence of plant nutritional status and soil chemistry on microbial community composition.
29957342	5	20	theme	nutritional	783:793	arg1	status					795:800	plant nutritional status	777:800	plant nutritional status	777:800	Additionally, chemical analyses of soils and grape leaves were used to explore the influence of plant nutritional status and soil chemistry on microbial community composition.
29957342	6	21	theme	predicted	939:947	arg1	function					969:976	predicted bacterial community function	939:976	predicted bacterial community function	939:976	Finally, factors influencing bacterial community composition were correlated with predicted bacterial community function.
29957342	10	22	theme	leaf	1786:1789	arg1	Mg					1791:1792	leaf Mg	1786:1792	leaf Mg	1786:1792	Additionally, abundance of many bacterial operational taxonomic units was significantly correlated with leaf Mg, including those from the following orders: Rhodobacterales, Acidobacteriales, Opitutales, Sphingomonadales, Burkholderiales, Saprospirales, and Flavobacteriales.
29957342	9	23	theme	carbon	1475:1480	arg1	metabolism					1486:1495	carbon (C) metabolism	1475:1495	carbon (C) metabolism	1475:1495	Chlorosis rank was moderately correlated with KEGG Orthology (KO) terms associated with nitrogen (N) and carbon (C) metabolism in soils, while leaf Mg was associated with a spectrum of KO terms including glycosphingolipid biosynthesis, glycan degradation, transporters, and porphyrin and chlorophyll metabolism.
29957342	11	24	theme	soil	2089:2092	arg1	chemistry					2094:2102	soil chemistry	2089:2102	soil chemistry	2089:2102	Our findings suggest grapevine chlorosis is interrelated with soil microbial community structure and function, plant nutrition, and soil chemistry.
29957342	8	25	theme	leaf	1340:1343	arg1	N					1345:1345	leaf N	1340:1345	leaf N	1340:1345	Non-metric multidimensional scaling (NMDS) revealed a significant correlation between fungal community composition and soil Fe and pH, along with leaf N, Mg, and Ca (mg.kg-1).
29957342	11	26	theme	microbial	2024:2032	arg1	structure					2044:2052	soil microbial community structure	2019:2052	soil microbial community structure	2019:2052	Our findings suggest grapevine chlorosis is interrelated with soil microbial community structure and function, plant nutrition, and soil chemistry.
29957342	4	27	theme	visual	654:659	arg1	symptoms					671:678	visual chlorosis symptoms	654:678	visual chlorosis symptoms	654:678	We used next-generation sequencing (NGS) to examine the bacterial and fungal communities associated with grapevines demonstrating varying degrees of visual chlorosis symptoms.
29957342	3	28	from	Physiology	343:352	arg1	vineyards					370:378	vineyards	370:378	vineyards	370:378	Physiology of chlorosis in vineyards has been well-studied, but the soil microbial consequences of and contributions to chlorosis have received little attention.
29957342	7	29	theme	tissue	984:989	arg1	magnesium					991:999	Leaf tissue magnesium	979:999	Leaf tissue magnesium (leaf Mg) concentrations	979:1024	Leaf tissue magnesium (leaf Mg) concentrations and chlorosis rank were correlated with bacterial community composition as determined via dbRDA (distance-based Redundancy Analysis) using Bray-Curtis dissimilarities.
29957342	7	29	theme	tissue	984:989	arg1	Mg					1007:1008	leaf Mg	1002:1008	leaf Mg	1002:1008	Leaf tissue magnesium (leaf Mg) concentrations and chlorosis rank were correlated with bacterial community composition as determined via dbRDA (distance-based Redundancy Analysis) using Bray-Curtis dissimilarities.
29957342	4	30	theme	symptoms	671:678	arg1	degrees					643:649	varying degrees	635:649	varying degrees of visual chlorosis symptoms	635:678	We used next-generation sequencing (NGS) to examine the bacterial and fungal communities associated with grapevines demonstrating varying degrees of visual chlorosis symptoms.
29957342	7	31	theme	distance-based	1123:1136	arg1	dbRDA					1116:1120	dbRDA	1116:1120	dbRDA (distance-based Redundancy Analysis)	1116:1157	Leaf tissue magnesium (leaf Mg) concentrations and chlorosis rank were correlated with bacterial community composition as determined via dbRDA (distance-based Redundancy Analysis) using Bray-Curtis dissimilarities.
29957342	7	31	theme	distance-based	1123:1136	arg1	Analysis					1149:1156	distance-based Redundancy Analysis	1123:1156	distance-based Redundancy Analysis	1123:1156	Leaf tissue magnesium (leaf Mg) concentrations and chlorosis rank were correlated with bacterial community composition as determined via dbRDA (distance-based Redundancy Analysis) using Bray-Curtis dissimilarities.
29957342	11	32	theme	community	2034:2042	arg1	structure					2044:2052	soil microbial community structure	2019:2052	soil microbial community structure	2019:2052	Our findings suggest grapevine chlorosis is interrelated with soil microbial community structure and function, plant nutrition, and soil chemistry.
29957342	0	33	theme	root	95:98	arg1	microbiomes					105:115	fungal and bacterial root zone microbiomes	74:115	fungal and bacterial root zone microbiomes	74:115	'Concord' grapevine nutritional status and chlorosis rank associated with fungal and bacterial root zone microbiomes.
29957342	5	34	theme	plant	777:781	arg1	status					795:800	plant nutritional status	777:800	plant nutritional status	777:800	Additionally, chemical analyses of soils and grape leaves were used to explore the influence of plant nutritional status and soil chemistry on microbial community composition.
29957342	5	35	theme	status	795:800	arg1	influence					764:772	the influence	760:772	the influence of plant nutritional status and soil chemistry on microbial community composition	760:854	Additionally, chemical analyses of soils and grape leaves were used to explore the influence of plant nutritional status and soil chemistry on microbial community composition.
29957342	10	36	theme	operational	1724:1734	arg1	units					1746:1750	many bacterial operational taxonomic units	1709:1750	many bacterial operational taxonomic units	1709:1750	Additionally, abundance of many bacterial operational taxonomic units was significantly correlated with leaf Mg, including those from the following orders: Rhodobacterales, Acidobacteriales, Opitutales, Sphingomonadales, Burkholderiales, Saprospirales, and Flavobacteriales.
29957342	3	37	theme	chlorosis	357:365	arg1	Physiology					343:352	Physiology	343:352	Physiology of chlorosis in vineyards	343:378	Physiology of chlorosis in vineyards has been well-studied, but the soil microbial consequences of and contributions to chlorosis have received little attention.
29957342	4	38	theme	next-generation	513:527	arg1	NGS					541:543	NGS	541:543	NGS	541:543	We used next-generation sequencing (NGS) to examine the bacterial and fungal communities associated with grapevines demonstrating varying degrees of visual chlorosis symptoms.
29957342	4	38	theme	next-generation	513:527	arg1	sequencing					529:538	next-generation sequencing	513:538	next-generation sequencing (NGS)	513:544	We used next-generation sequencing (NGS) to examine the bacterial and fungal communities associated with grapevines demonstrating varying degrees of visual chlorosis symptoms.
29957342	0	39	theme	nutritional	20:30	arg1	status					32:37	'Concord' grapevine nutritional status	0:37	'Concord' grapevine nutritional status	0:37	'Concord' grapevine nutritional status and chlorosis rank associated with fungal and bacterial root zone microbiomes.
29957342	9	40	theme	Chlorosis	1370:1378	arg1	rank					1380:1383	Chlorosis rank	1370:1383	Chlorosis rank	1370:1383	Chlorosis rank was moderately correlated with KEGG Orthology (KO) terms associated with nitrogen (N) and carbon (C) metabolism in soils, while leaf Mg was associated with a spectrum of KO terms including glycosphingolipid biosynthesis, glycan degradation, transporters, and porphyrin and chlorophyll metabolism.
29957342	9	41	theme	terms	1558:1562	arg1	spectrum					1543:1550	a spectrum	1541:1550	a spectrum of KO terms including glycosphingolipid biosynthesis, glycan degradation, transporters, and porphyrin and chlorophyll metabolism	1541:1679	Chlorosis rank was moderately correlated with KEGG Orthology (KO) terms associated with nitrogen (N) and carbon (C) metabolism in soils, while leaf Mg was associated with a spectrum of KO terms including glycosphingolipid biosynthesis, glycan degradation, transporters, and porphyrin and chlorophyll metabolism.
29957342	9	42	from	nitrogen	1458:1465	arg1	soils					1500:1504	soils	1500:1504	soils	1500:1504	Chlorosis rank was moderately correlated with KEGG Orthology (KO) terms associated with nitrogen (N) and carbon (C) metabolism in soils, while leaf Mg was associated with a spectrum of KO terms including glycosphingolipid biosynthesis, glycan degradation, transporters, and porphyrin and chlorophyll metabolism.
29957342	3	43	theme	microbial	416:424	arg1	consequences					426:437	the soil microbial consequences	407:437	the soil microbial consequences of and contributions to chlorosis	407:471	Physiology of chlorosis in vineyards has been well-studied, but the soil microbial consequences of and contributions to chlorosis have received little attention.
29957342	10	44	theme	bacterial	1714:1722	arg1	units					1746:1750	many bacterial operational taxonomic units	1709:1750	many bacterial operational taxonomic units	1709:1750	Additionally, abundance of many bacterial operational taxonomic units was significantly correlated with leaf Mg, including those from the following orders: Rhodobacterales, Acidobacteriales, Opitutales, Sphingomonadales, Burkholderiales, Saprospirales, and Flavobacteriales.
29957342	9	45	theme	glycosphingolipid	1574:1590	arg1	biosynthesis					1592:1603	glycosphingolipid biosynthesis	1574:1603	glycosphingolipid biosynthesis	1574:1603	Chlorosis rank was moderately correlated with KEGG Orthology (KO) terms associated with nitrogen (N) and carbon (C) metabolism in soils, while leaf Mg was associated with a spectrum of KO terms including glycosphingolipid biosynthesis, glycan degradation, transporters, and porphyrin and chlorophyll metabolism.
29957342	10	46	theme	many	1709:1712	arg1	units					1746:1750	many bacterial operational taxonomic units	1709:1750	many bacterial operational taxonomic units	1709:1750	Additionally, abundance of many bacterial operational taxonomic units was significantly correlated with leaf Mg, including those from the following orders: Rhodobacterales, Acidobacteriales, Opitutales, Sphingomonadales, Burkholderiales, Saprospirales, and Flavobacteriales.
29957342	5	47	theme	community	834:842	arg1	composition					844:854	microbial community composition	824:854	microbial community composition	824:854	Additionally, chemical analyses of soils and grape leaves were used to explore the influence of plant nutritional status and soil chemistry on microbial community composition.
29957342	4	48	theme	bacterial	561:569	arg1	communities					582:592	the bacterial and fungal communities	557:592	the bacterial and fungal communities associated with grapevines demonstrating varying degrees of visual chlorosis symptoms	557:678	We used next-generation sequencing (NGS) to examine the bacterial and fungal communities associated with grapevines demonstrating varying degrees of visual chlorosis symptoms.
29957342	7	49	theme	Redundancy	1138:1147	arg1	dbRDA					1116:1120	dbRDA	1116:1120	dbRDA (distance-based Redundancy Analysis)	1116:1157	Leaf tissue magnesium (leaf Mg) concentrations and chlorosis rank were correlated with bacterial community composition as determined via dbRDA (distance-based Redundancy Analysis) using Bray-Curtis dissimilarities.
29957342	7	49	theme	Redundancy	1138:1147	arg1	Analysis					1149:1156	distance-based Redundancy Analysis	1123:1156	distance-based Redundancy Analysis	1123:1156	Leaf tissue magnesium (leaf Mg) concentrations and chlorosis rank were correlated with bacterial community composition as determined via dbRDA (distance-based Redundancy Analysis) using Bray-Curtis dissimilarities.
29957342	3	50	theme	contributions	446:458	arg1	consequences					426:437	the soil microbial consequences	407:437	the soil microbial consequences of and contributions to chlorosis	407:471	Physiology of chlorosis in vineyards has been well-studied, but the soil microbial consequences of and contributions to chlorosis have received little attention.
29957342	9	51	theme	KEGG	1416:1419	arg1	terms					1436:1440	KEGG Orthology (KO) terms	1416:1440	KEGG Orthology (KO) terms associated with nitrogen (N) and carbon (C) metabolism in soils	1416:1504	Chlorosis rank was moderately correlated with KEGG Orthology (KO) terms associated with nitrogen (N) and carbon (C) metabolism in soils, while leaf Mg was associated with a spectrum of KO terms including glycosphingolipid biosynthesis, glycan degradation, transporters, and porphyrin and chlorophyll metabolism.
29957342	6	52	theme	community	896:904	arg1	composition					906:916	bacterial community composition	886:916	bacterial community composition	886:916	Finally, factors influencing bacterial community composition were correlated with predicted bacterial community function.
29957342	10	53	dep	orders	1830:1835	arg1	Sphingomonadales					1885:1900	Sphingomonadales	1885:1900	Sphingomonadales	1885:1900	Additionally, abundance of many bacterial operational taxonomic units was significantly correlated with leaf Mg, including those from the following orders: Rhodobacterales, Acidobacteriales, Opitutales, Sphingomonadales, Burkholderiales, Saprospirales, and Flavobacteriales.
29957342	10	53	dep	orders	1830:1835	arg1	orders					1830:1835	the following orders	1816:1835	the following orders: Rhodobacterales, Acidobacteriales, Opitutales, Sphingomonadales, Burkholderiales, Saprospirales, and Flavobacteriales	1816:1954	Additionally, abundance of many bacterial operational taxonomic units was significantly correlated with leaf Mg, including those from the following orders: Rhodobacterales, Acidobacteriales, Opitutales, Sphingomonadales, Burkholderiales, Saprospirales, and Flavobacteriales.
29957342	10	53	dep	orders	1830:1835	arg1	Acidobacteriales					1855:1870	Acidobacteriales	1855:1870	Acidobacteriales	1855:1870	Additionally, abundance of many bacterial operational taxonomic units was significantly correlated with leaf Mg, including those from the following orders: Rhodobacterales, Acidobacteriales, Opitutales, Sphingomonadales, Burkholderiales, Saprospirales, and Flavobacteriales.
29957342	10	53	dep	orders	1830:1835	arg1	Saprospirales					1920:1932	Saprospirales	1920:1932	Saprospirales	1920:1932	Additionally, abundance of many bacterial operational taxonomic units was significantly correlated with leaf Mg, including those from the following orders: Rhodobacterales, Acidobacteriales, Opitutales, Sphingomonadales, Burkholderiales, Saprospirales, and Flavobacteriales.
29957342	10	53	dep	orders	1830:1835	arg1	Flavobacteriales					1939:1954	Flavobacteriales	1939:1954	Flavobacteriales	1939:1954	Additionally, abundance of many bacterial operational taxonomic units was significantly correlated with leaf Mg, including those from the following orders: Rhodobacterales, Acidobacteriales, Opitutales, Sphingomonadales, Burkholderiales, Saprospirales, and Flavobacteriales.
29957342	10	54	theme	units	1746:1750	arg1	abundance					1696:1704	abundance	1696:1704	abundance of many bacterial operational taxonomic units	1696:1750	Additionally, abundance of many bacterial operational taxonomic units was significantly correlated with leaf Mg, including those from the following orders: Rhodobacterales, Acidobacteriales, Opitutales, Sphingomonadales, Burkholderiales, Saprospirales, and Flavobacteriales.
29957342	2	55	theme	crucial	238:244	arg1	role					246:249	a crucial role	236:249	a crucial role	236:249	While iron (Fe) is understood to play a crucial role in chlorosis, total plant and soil Fe are not always indicative of chlorosis in grapevines.
29957342	5	56	theme	microbial	824:832	arg1	composition					844:854	microbial community composition	824:854	microbial community composition	824:854	Additionally, chemical analyses of soils and grape leaves were used to explore the influence of plant nutritional status and soil chemistry on microbial community composition.
29957342	8	57	theme	multidimensional	1205:1220	arg1	NMDS					1231:1234	NMDS	1231:1234	NMDS	1231:1234	Non-metric multidimensional scaling (NMDS) revealed a significant correlation between fungal community composition and soil Fe and pH, along with leaf N, Mg, and Ca (mg.kg-1).
29957342	8	57	theme	multidimensional	1205:1220	arg1	scaling					1222:1228	Non-metric multidimensional scaling	1194:1228	Non-metric multidimensional scaling (NMDS)	1194:1235	Non-metric multidimensional scaling (NMDS) revealed a significant correlation between fungal community composition and soil Fe and pH, along with leaf N, Mg, and Ca (mg.kg-1).
29957342	8	58	theme	fungal	1280:1285	arg1	composition					1297:1307	fungal community composition	1280:1307	fungal community composition	1280:1307	Non-metric multidimensional scaling (NMDS) revealed a significant correlation between fungal community composition and soil Fe and pH, along with leaf N, Mg, and Ca (mg.kg-1).
29957342	1	59	theme	crop	173:176	arg1	yields					178:183	reduced crop yields	165:183	reduced crop yields	165:183	Leaf chlorosis in vineyards is associated with reduced crop yields and quality.
29957342	4	60	theme	varying	635:641	arg1	degrees					643:649	varying degrees	635:649	varying degrees of visual chlorosis symptoms	635:678	We used next-generation sequencing (NGS) to examine the bacterial and fungal communities associated with grapevines demonstrating varying degrees of visual chlorosis symptoms.
29957342	7	61	theme	community	1076:1084	arg1	composition					1086:1096	bacterial community composition	1066:1096	bacterial community composition	1066:1096	Leaf tissue magnesium (leaf Mg) concentrations and chlorosis rank were correlated with bacterial community composition as determined via dbRDA (distance-based Redundancy Analysis) using Bray-Curtis dissimilarities.
29957342	4	62	theme	fungal	575:580	arg1	communities					582:592	the bacterial and fungal communities	557:592	the bacterial and fungal communities associated with grapevines demonstrating varying degrees of visual chlorosis symptoms	557:678	We used next-generation sequencing (NGS) to examine the bacterial and fungal communities associated with grapevines demonstrating varying degrees of visual chlorosis symptoms.
29957342	5	63	theme	chemistry	811:819	arg1	influence					764:772	the influence	760:772	the influence of plant nutritional status and soil chemistry on microbial community composition	760:854	Additionally, chemical analyses of soils and grape leaves were used to explore the influence of plant nutritional status and soil chemistry on microbial community composition.
29957342	6	64	theme	bacterial	949:957	arg1	function					969:976	predicted bacterial community function	939:976	predicted bacterial community function	939:976	Finally, factors influencing bacterial community composition were correlated with predicted bacterial community function.
29957342	7	65	theme	chlorosis	1030:1038	arg1	rank					1040:1043	chlorosis rank	1030:1043	chlorosis rank	1030:1043	Leaf tissue magnesium (leaf Mg) concentrations and chlorosis rank were correlated with bacterial community composition as determined via dbRDA (distance-based Redundancy Analysis) using Bray-Curtis dissimilarities.
29957342	10	66	theme	following	1820:1828	arg1	orders					1830:1835	the following orders	1816:1835	the following orders: Rhodobacterales, Acidobacteriales, Opitutales, Sphingomonadales, Burkholderiales, Saprospirales, and Flavobacteriales	1816:1954	Additionally, abundance of many bacterial operational taxonomic units was significantly correlated with leaf Mg, including those from the following orders: Rhodobacterales, Acidobacteriales, Opitutales, Sphingomonadales, Burkholderiales, Saprospirales, and Flavobacteriales.
29957342	10	66	theme	following	1820:1828	arg1	Sphingomonadales					1885:1900	Sphingomonadales	1885:1900	Sphingomonadales	1885:1900	Additionally, abundance of many bacterial operational taxonomic units was significantly correlated with leaf Mg, including those from the following orders: Rhodobacterales, Acidobacteriales, Opitutales, Sphingomonadales, Burkholderiales, Saprospirales, and Flavobacteriales.
29957342	10	66	theme	following	1820:1828	arg1	Acidobacteriales					1855:1870	Acidobacteriales	1855:1870	Acidobacteriales	1855:1870	Additionally, abundance of many bacterial operational taxonomic units was significantly correlated with leaf Mg, including those from the following orders: Rhodobacterales, Acidobacteriales, Opitutales, Sphingomonadales, Burkholderiales, Saprospirales, and Flavobacteriales.
29957342	10	66	theme	following	1820:1828	arg1	Saprospirales					1920:1932	Saprospirales	1920:1932	Saprospirales	1920:1932	Additionally, abundance of many bacterial operational taxonomic units was significantly correlated with leaf Mg, including those from the following orders: Rhodobacterales, Acidobacteriales, Opitutales, Sphingomonadales, Burkholderiales, Saprospirales, and Flavobacteriales.
29957342	10	66	theme	following	1820:1828	arg1	Flavobacteriales					1939:1954	Flavobacteriales	1939:1954	Flavobacteriales	1939:1954	Additionally, abundance of many bacterial operational taxonomic units was significantly correlated with leaf Mg, including those from the following orders: Rhodobacterales, Acidobacteriales, Opitutales, Sphingomonadales, Burkholderiales, Saprospirales, and Flavobacteriales.
29957342	1	67	theme	Leaf	118:121	arg1	chlorosis					123:131	Leaf chlorosis	118:131	Leaf chlorosis in vineyards	118:144	Leaf chlorosis in vineyards is associated with reduced crop yields and quality.
29957342	2	68	theme	soil	281:284	arg1	Fe					286:287	soil Fe	281:287	soil Fe	281:287	While iron (Fe) is understood to play a crucial role in chlorosis, total plant and soil Fe are not always indicative of chlorosis in grapevines.
29957342	5	69	theme	grape	726:730	arg1	analyses					704:711	chemical analyses	695:711	chemical analyses of soils and grape leaves	695:737	Additionally, chemical analyses of soils and grape leaves were used to explore the influence of plant nutritional status and soil chemistry on microbial community composition.
29957342	11	70	theme	grapevine	1978:1986	arg1	chlorosis					1988:1996	grapevine chlorosis	1978:1996	grapevine chlorosis	1978:1996	Our findings suggest grapevine chlorosis is interrelated with soil microbial community structure and function, plant nutrition, and soil chemistry.
29957342	7	71	theme	leaf	1002:1005	arg1	magnesium					991:999	Leaf tissue magnesium	979:999	Leaf tissue magnesium (leaf Mg) concentrations	979:1024	Leaf tissue magnesium (leaf Mg) concentrations and chlorosis rank were correlated with bacterial community composition as determined via dbRDA (distance-based Redundancy Analysis) using Bray-Curtis dissimilarities.
29957342	7	71	theme	leaf	1002:1005	arg1	Mg					1007:1008	leaf Mg	1002:1008	leaf Mg	1002:1008	Leaf tissue magnesium (leaf Mg) concentrations and chlorosis rank were correlated with bacterial community composition as determined via dbRDA (distance-based Redundancy Analysis) using Bray-Curtis dissimilarities.
29957342	0	72	theme	fungal	74:79	arg1	microbiomes					105:115	fungal and bacterial root zone microbiomes	74:115	fungal and bacterial root zone microbiomes	74:115	'Concord' grapevine nutritional status and chlorosis rank associated with fungal and bacterial root zone microbiomes.
29957342	5	73	from	influence	764:772	arg1	composition					844:854	microbial community composition	824:854	microbial community composition	824:854	Additionally, chemical analyses of soils and grape leaves were used to explore the influence of plant nutritional status and soil chemistry on microbial community composition.
29957342	7	74	theme	magnesium	991:999	arg1	concentrations					1011:1024	Leaf tissue magnesium (leaf Mg) concentrations	979:1024	Leaf tissue magnesium (leaf Mg) concentrations	979:1024	Leaf tissue magnesium (leaf Mg) concentrations and chlorosis rank were correlated with bacterial community composition as determined via dbRDA (distance-based Redundancy Analysis) using Bray-Curtis dissimilarities.
29957342	4	75	theme	chlorosis	661:669	arg1	symptoms					671:678	visual chlorosis symptoms	654:678	visual chlorosis symptoms	654:678	We used next-generation sequencing (NGS) to examine the bacterial and fungal communities associated with grapevines demonstrating varying degrees of visual chlorosis symptoms.
29957342	0	76	theme	bacterial	85:93	arg1	microbiomes					105:115	fungal and bacterial root zone microbiomes	74:115	fungal and bacterial root zone microbiomes	74:115	'Concord' grapevine nutritional status and chlorosis rank associated with fungal and bacterial root zone microbiomes.
29957342	7	77	theme	Leaf	979:982	arg1	magnesium					991:999	Leaf tissue magnesium	979:999	Leaf tissue magnesium (leaf Mg) concentrations	979:1024	Leaf tissue magnesium (leaf Mg) concentrations and chlorosis rank were correlated with bacterial community composition as determined via dbRDA (distance-based Redundancy Analysis) using Bray-Curtis dissimilarities.
29957342	7	77	theme	Leaf	979:982	arg1	Mg					1007:1008	leaf Mg	1002:1008	leaf Mg	1002:1008	Leaf tissue magnesium (leaf Mg) concentrations and chlorosis rank were correlated with bacterial community composition as determined via dbRDA (distance-based Redundancy Analysis) using Bray-Curtis dissimilarities.
29957342	0	78	theme	zone	100:103	arg1	microbiomes					105:115	fungal and bacterial root zone microbiomes	74:115	fungal and bacterial root zone microbiomes	74:115	'Concord' grapevine nutritional status and chlorosis rank associated with fungal and bacterial root zone microbiomes.
29957342	5	79	used	used	744:747	arg2	analyses					704:711	chemical analyses	695:711	chemical analyses of soils and grape leaves	695:737	Additionally, chemical analyses of soils and grape leaves were used to explore the influence of plant nutritional status and soil chemistry on microbial community composition.
29957342	8	80	theme	soil	1313:1316	arg1	Fe					1318:1319	soil Fe	1313:1319	soil Fe	1313:1319	Non-metric multidimensional scaling (NMDS) revealed a significant correlation between fungal community composition and soil Fe and pH, along with leaf N, Mg, and Ca (mg.kg-1).
29957342	9	81	theme	leaf	1513:1516	arg1	Mg					1518:1519	leaf Mg	1513:1519	leaf Mg	1513:1519	Chlorosis rank was moderately correlated with KEGG Orthology (KO) terms associated with nitrogen (N) and carbon (C) metabolism in soils, while leaf Mg was associated with a spectrum of KO terms including glycosphingolipid biosynthesis, glycan degradation, transporters, and porphyrin and chlorophyll metabolism.
29957342	5	82	theme	soils	716:720	arg1	analyses					704:711	chemical analyses	695:711	chemical analyses of soils and grape leaves	695:737	Additionally, chemical analyses of soils and grape leaves were used to explore the influence of plant nutritional status and soil chemistry on microbial community composition.
29957342	0	83	theme	Concord	1:7	arg1	status					32:37	'Concord' grapevine nutritional status	0:37	'Concord' grapevine nutritional status	0:37	'Concord' grapevine nutritional status and chlorosis rank associated with fungal and bacterial root zone microbiomes.
29957342	1	84	from	chlorosis	123:131	arg1	vineyards					136:144	vineyards	136:144	vineyards	136:144	Leaf chlorosis in vineyards is associated with reduced crop yields and quality.
29957342	11	85	theme	plant	2068:2072	arg1	nutrition					2074:2082	plant nutrition	2068:2082	plant nutrition	2068:2082	Our findings suggest grapevine chlorosis is interrelated with soil microbial community structure and function, plant nutrition, and soil chemistry.
29957342	2	86	theme	chlorosis	318:326	arg1	indicative					304:313	indicative	304:313	indicative	304:313	While iron (Fe) is understood to play a crucial role in chlorosis, total plant and soil Fe are not always indicative of chlorosis in grapevines.
29957342	0	87	theme	grapevine	10:18	arg1	status					32:37	'Concord' grapevine nutritional status	0:37	'Concord' grapevine nutritional status	0:37	'Concord' grapevine nutritional status and chlorosis rank associated with fungal and bacterial root zone microbiomes.
29957342	9	88	dep	metabolism	1486:1495	arg1	C					1483:1483	C	1483:1483	C	1483:1483	Chlorosis rank was moderately correlated with KEGG Orthology (KO) terms associated with nitrogen (N) and carbon (C) metabolism in soils, while leaf Mg was associated with a spectrum of KO terms including glycosphingolipid biosynthesis, glycan degradation, transporters, and porphyrin and chlorophyll metabolism.
29957342	3	89	theme	soil	411:414	arg1	consequences					426:437	the soil microbial consequences	407:437	the soil microbial consequences of and contributions to chlorosis	407:471	Physiology of chlorosis in vineyards has been well-studied, but the soil microbial consequences of and contributions to chlorosis have received little attention.
29957342	7	90	theme	Bray-Curtis	1165:1175	arg1	dissimilarities					1177:1191	Bray-Curtis dissimilarities	1165:1191	Bray-Curtis dissimilarities	1165:1191	Leaf tissue magnesium (leaf Mg) concentrations and chlorosis rank were correlated with bacterial community composition as determined via dbRDA (distance-based Redundancy Analysis) using Bray-Curtis dissimilarities.
29957342	0	91	theme	chlorosis	43:51	arg1	rank					53:56	chlorosis rank	43:56	chlorosis rank	43:56	'Concord' grapevine nutritional status and chlorosis rank associated with fungal and bacterial root zone microbiomes.
29957342	9	92	theme	KO	1555:1556	arg1	terms					1558:1562	KO terms	1555:1562	KO terms including glycosphingolipid biosynthesis, glycan degradation, transporters, and porphyrin and chlorophyll metabolism	1555:1679	Chlorosis rank was moderately correlated with KEGG Orthology (KO) terms associated with nitrogen (N) and carbon (C) metabolism in soils, while leaf Mg was associated with a spectrum of KO terms including glycosphingolipid biosynthesis, glycan degradation, transporters, and porphyrin and chlorophyll metabolism.
29957342	9	92	theme	KO	1555:1556	arg1	biosynthesis					1592:1603	glycosphingolipid biosynthesis	1574:1603	glycosphingolipid biosynthesis	1574:1603	Chlorosis rank was moderately correlated with KEGG Orthology (KO) terms associated with nitrogen (N) and carbon (C) metabolism in soils, while leaf Mg was associated with a spectrum of KO terms including glycosphingolipid biosynthesis, glycan degradation, transporters, and porphyrin and chlorophyll metabolism.
29957342	9	92	theme	KO	1555:1556	arg1	metabolism					1670:1679	porphyrin and chlorophyll metabolism	1644:1679	metabolism	1670:1679	Chlorosis rank was moderately correlated with KEGG Orthology (KO) terms associated with nitrogen (N) and carbon (C) metabolism in soils, while leaf Mg was associated with a spectrum of KO terms including glycosphingolipid biosynthesis, glycan degradation, transporters, and porphyrin and chlorophyll metabolism.
29957342	9	92	theme	KO	1555:1556	arg1	transporters					1626:1637	transporters	1626:1637	transporters	1626:1637	Chlorosis rank was moderately correlated with KEGG Orthology (KO) terms associated with nitrogen (N) and carbon (C) metabolism in soils, while leaf Mg was associated with a spectrum of KO terms including glycosphingolipid biosynthesis, glycan degradation, transporters, and porphyrin and chlorophyll metabolism.
29957342	9	92	theme	KO	1555:1556	arg1	degradation					1613:1623	glycan degradation	1606:1623	glycan degradation	1606:1623	Chlorosis rank was moderately correlated with KEGG Orthology (KO) terms associated with nitrogen (N) and carbon (C) metabolism in soils, while leaf Mg was associated with a spectrum of KO terms including glycosphingolipid biosynthesis, glycan degradation, transporters, and porphyrin and chlorophyll metabolism.
29957342	2	93	from	chlorosis	318:326	arg1	grapevines					331:340	grapevines	331:340	grapevines	331:340	While iron (Fe) is understood to play a crucial role in chlorosis, total plant and soil Fe are not always indicative of chlorosis in grapevines.
29957342	7	94	theme	bacterial	1066:1074	arg1	composition					1086:1096	bacterial community composition	1066:1096	bacterial community composition	1066:1096	Leaf tissue magnesium (leaf Mg) concentrations and chlorosis rank were correlated with bacterial community composition as determined via dbRDA (distance-based Redundancy Analysis) using Bray-Curtis dissimilarities.
29957342	11	95	theme	soil	2019:2022	arg1	structure					2044:2052	soil microbial community structure	2019:2052	soil microbial community structure	2019:2052	Our findings suggest grapevine chlorosis is interrelated with soil microbial community structure and function, plant nutrition, and soil chemistry.
30605248	2	0	theme	nutrient	472:479	arg1	composition					481:491	their nutrient composition	466:491	their nutrient composition	466:491	Many existing studies on truffles focus on analyzing their taxonomy, population genetics, volatile organic compounds and artificial cultivation of the truffles, while little information is available about their nutrient composition and pharmacological activity, especially the relationship between chemical composition in ascocarps and their geographic distributions.
30605248	3	1	theme	free	732:735	arg1	province					992:999	Liaoning province	983:999	Liaoning province	983:999	This study presents a comprehensive investigation of the chemical composition of T. indicum, including free sugars, fatty acids, organic acids, phenolic acids, flavonoids, and polysaccharides, and tracks the antioxidant activity of T. indicum ascocarps collected from five geographical regions of four provinces in P. R. China: Hebei, Tibet, Yunnan, and Liaoning province.
30605248	3	1	theme	free	732:735	arg1	activity					849:856	the antioxidant activity	833:856	the antioxidant activity of T. indicum ascocarps collected from five geographical regions of four provinces in P. R. China	833:954	This study presents a comprehensive investigation of the chemical composition of T. indicum, including free sugars, fatty acids, organic acids, phenolic acids, flavonoids, and polysaccharides, and tracks the antioxidant activity of T. indicum ascocarps collected from five geographical regions of four provinces in P. R. China: Hebei, Tibet, Yunnan, and Liaoning province.
30605248	3	1	theme	free	732:735	arg1	Tibet					964:968	Tibet	964:968	Tibet	964:968	This study presents a comprehensive investigation of the chemical composition of T. indicum, including free sugars, fatty acids, organic acids, phenolic acids, flavonoids, and polysaccharides, and tracks the antioxidant activity of T. indicum ascocarps collected from five geographical regions of four provinces in P. R. China: Hebei, Tibet, Yunnan, and Liaoning province.
30605248	3	1	theme	free	732:735	arg1	Yunnan					971:976	Yunnan	971:976	Yunnan	971:976	This study presents a comprehensive investigation of the chemical composition of T. indicum, including free sugars, fatty acids, organic acids, phenolic acids, flavonoids, and polysaccharides, and tracks the antioxidant activity of T. indicum ascocarps collected from five geographical regions of four provinces in P. R. China: Hebei, Tibet, Yunnan, and Liaoning province.
30605248	3	1	theme	free	732:735	arg1	Hebei					957:961	Hebei	957:961	Hebei	957:961	This study presents a comprehensive investigation of the chemical composition of T. indicum, including free sugars, fatty acids, organic acids, phenolic acids, flavonoids, and polysaccharides, and tracks the antioxidant activity of T. indicum ascocarps collected from five geographical regions of four provinces in P. R. China: Hebei, Tibet, Yunnan, and Liaoning province.
30605248	3	1	theme	free	732:735	arg1	sugars					737:742	free sugars	732:742	free sugars	732:742	This study presents a comprehensive investigation of the chemical composition of T. indicum, including free sugars, fatty acids, organic acids, phenolic acids, flavonoids, and polysaccharides, and tracks the antioxidant activity of T. indicum ascocarps collected from five geographical regions of four provinces in P. R. China: Hebei, Tibet, Yunnan, and Liaoning province.
30605248	7	2	theme	chemical	1660:1667	arg1	composition					1669:1679	chemical composition	1660:1679	chemical composition	1660:1679	Detecting differences in chemical composition may provide important data for understanding the relationship between environmental factors and truffle formation, as well as quality evaluation of the commercial species T. indicum throughout China.
30605248	7	3	theme	species	1844:1850	arg1	evaluation					1815:1824	quality evaluation	1807:1824	quality evaluation of the commercial species	1807:1850	Detecting differences in chemical composition may provide important data for understanding the relationship between environmental factors and truffle formation, as well as quality evaluation of the commercial species T. indicum throughout China.
30605248	7	3	theme	species	1844:1850	arg1	relationship					1730:1741	the relationship	1726:1741	the relationship between environmental factors and truffle formation	1726:1793	Detecting differences in chemical composition may provide important data for understanding the relationship between environmental factors and truffle formation, as well as quality evaluation of the commercial species T. indicum throughout China.
30605248	2	4	theme	population	330:339	arg1	genetics					341:348	population genetics	330:348	population genetics	330:348	Many existing studies on truffles focus on analyzing their taxonomy, population genetics, volatile organic compounds and artificial cultivation of the truffles, while little information is available about their nutrient composition and pharmacological activity, especially the relationship between chemical composition in ascocarps and their geographic distributions.
30605248	2	5	from	studies	275:281	arg1	truffles					286:293	truffles	286:293	truffles	286:293	Many existing studies on truffles focus on analyzing their taxonomy, population genetics, volatile organic compounds and artificial cultivation of the truffles, while little information is available about their nutrient composition and pharmacological activity, especially the relationship between chemical composition in ascocarps and their geographic distributions.
30605248	0	6	theme	Geographical	78:89	arg1	Regions					91:97	Different Geographical Regions	68:97	Different Geographical Regions of China	68:106	Chemical Composition and Antioxidant Activity of Tuber indicum from Different Geographical Regions of China.
30605248	7	7	theme	Detecting	1635:1643	arg1	differences					1645:1655	Detecting differences	1635:1655	Detecting differences in chemical composition	1635:1679	Detecting differences in chemical composition may provide important data for understanding the relationship between environmental factors and truffle formation, as well as quality evaluation of the commercial species T. indicum throughout China.
30605248	7	8	theme	truffle	1777:1783	arg1	formation					1785:1793	truffle formation	1777:1793	truffle formation	1777:1793	Detecting differences in chemical composition may provide important data for understanding the relationship between environmental factors and truffle formation, as well as quality evaluation of the commercial species T. indicum throughout China.
30605248	3	9	theme	ascocarps	872:880	arg1	province					992:999	Liaoning province	983:999	Liaoning province	983:999	This study presents a comprehensive investigation of the chemical composition of T. indicum, including free sugars, fatty acids, organic acids, phenolic acids, flavonoids, and polysaccharides, and tracks the antioxidant activity of T. indicum ascocarps collected from five geographical regions of four provinces in P. R. China: Hebei, Tibet, Yunnan, and Liaoning province.
30605248	3	9	theme	ascocarps	872:880	arg1	activity					849:856	the antioxidant activity	833:856	the antioxidant activity of T. indicum ascocarps collected from five geographical regions of four provinces in P. R. China	833:954	This study presents a comprehensive investigation of the chemical composition of T. indicum, including free sugars, fatty acids, organic acids, phenolic acids, flavonoids, and polysaccharides, and tracks the antioxidant activity of T. indicum ascocarps collected from five geographical regions of four provinces in P. R. China: Hebei, Tibet, Yunnan, and Liaoning province.
30605248	3	9	theme	ascocarps	872:880	arg1	sugars					737:742	free sugars	732:742	free sugars	732:742	This study presents a comprehensive investigation of the chemical composition of T. indicum, including free sugars, fatty acids, organic acids, phenolic acids, flavonoids, and polysaccharides, and tracks the antioxidant activity of T. indicum ascocarps collected from five geographical regions of four provinces in P. R. China: Hebei, Tibet, Yunnan, and Liaoning province.
30605248	3	9	theme	ascocarps	872:880	arg1	Tibet					964:968	Tibet	964:968	Tibet	964:968	This study presents a comprehensive investigation of the chemical composition of T. indicum, including free sugars, fatty acids, organic acids, phenolic acids, flavonoids, and polysaccharides, and tracks the antioxidant activity of T. indicum ascocarps collected from five geographical regions of four provinces in P. R. China: Hebei, Tibet, Yunnan, and Liaoning province.
30605248	2	10	from	composition	568:578	arg1	distributions					614:626	their geographic distributions	597:626	their geographic distributions	597:626	Many existing studies on truffles focus on analyzing their taxonomy, population genetics, volatile organic compounds and artificial cultivation of the truffles, while little information is available about their nutrient composition and pharmacological activity, especially the relationship between chemical composition in ascocarps and their geographic distributions.
30605248	2	10	from	composition	568:578	arg1	ascocarps					583:591	ascocarps	583:591	ascocarps	583:591	Many existing studies on truffles focus on analyzing their taxonomy, population genetics, volatile organic compounds and artificial cultivation of the truffles, while little information is available about their nutrient composition and pharmacological activity, especially the relationship between chemical composition in ascocarps and their geographic distributions.
30605248	0	11	theme	China	102:106	arg1	Regions					91:97	Different Geographical Regions	68:97	Different Geographical Regions of China	68:106	Chemical Composition and Antioxidant Activity of Tuber indicum from Different Geographical Regions of China.
30605248	5	12	from	those	1504:1508	arg1	abundant					1492:1499	abundant	1492:1499	abundant	1492:1499	The amount of water-soluble polysaccharides was the highest (115.24 mg/g dw) in ascocarps from Tibet, the total organic acids was the highest (22.073 mg/g dw) in ascocarps from Gongshan, and polyunsaturated fatty acids were most abundant in those from Hebei province.
30605248	2	13	theme	geographic	603:612	arg1	distributions					614:626	their geographic distributions	597:626	their geographic distributions	597:626	Many existing studies on truffles focus on analyzing their taxonomy, population genetics, volatile organic compounds and artificial cultivation of the truffles, while little information is available about their nutrient composition and pharmacological activity, especially the relationship between chemical composition in ascocarps and their geographic distributions.
30605248	2	14	theme	existing	266:273	arg1	studies					275:281	Many existing studies	261:281	Many existing studies on truffles	261:293	Many existing studies on truffles focus on analyzing their taxonomy, population genetics, volatile organic compounds and artificial cultivation of the truffles, while little information is available about their nutrient composition and pharmacological activity, especially the relationship between chemical composition in ascocarps and their geographic distributions.
30605248	5	15	theme	Hebei	1515:1519	arg1	province					1521:1528	Hebei province	1515:1528	Hebei province	1515:1528	The amount of water-soluble polysaccharides was the highest (115.24 mg/g dw) in ascocarps from Tibet, the total organic acids was the highest (22.073 mg/g dw) in ascocarps from Gongshan, and polyunsaturated fatty acids were most abundant in those from Hebei province.
30605248	3	16	theme	fatty	745:749	arg1	acids					751:755	fatty acids	745:755	fatty acids	745:755	This study presents a comprehensive investigation of the chemical composition of T. indicum, including free sugars, fatty acids, organic acids, phenolic acids, flavonoids, and polysaccharides, and tracks the antioxidant activity of T. indicum ascocarps collected from five geographical regions of four provinces in P. R. China: Hebei, Tibet, Yunnan, and Liaoning province.
30605248	2	17	theme	Many	261:264	arg1	studies					275:281	Many existing studies	261:281	Many existing studies on truffles	261:293	Many existing studies on truffles focus on analyzing their taxonomy, population genetics, volatile organic compounds and artificial cultivation of the truffles, while little information is available about their nutrient composition and pharmacological activity, especially the relationship between chemical composition in ascocarps and their geographic distributions.
30605248	5	18	theme	polysaccharides	1291:1305	arg1	amount					1267:1272	The amount	1263:1272	The amount of water-soluble polysaccharides	1263:1305	The amount of water-soluble polysaccharides was the highest (115.24 mg/g dw) in ascocarps from Tibet, the total organic acids was the highest (22.073 mg/g dw) in ascocarps from Gongshan, and polyunsaturated fatty acids were most abundant in those from Hebei province.
30605248	5	18	theme	polysaccharides	1291:1305	arg1	polysaccharides					1291:1305	water-soluble polysaccharides	1277:1305	water-soluble polysaccharides	1277:1305	The amount of water-soluble polysaccharides was the highest (115.24 mg/g dw) in ascocarps from Tibet, the total organic acids was the highest (22.073 mg/g dw) in ascocarps from Gongshan, and polyunsaturated fatty acids were most abundant in those from Hebei province.
30605248	5	18	theme	polysaccharides	1291:1305	arg1	highest					1315:1321	highest	1315:1321	highest	1315:1321	The amount of water-soluble polysaccharides was the highest (115.24 mg/g dw) in ascocarps from Tibet, the total organic acids was the highest (22.073 mg/g dw) in ascocarps from Gongshan, and polyunsaturated fatty acids were most abundant in those from Hebei province.
30605248	3	19	dep	T.	710:711	arg1	indicum					713:719	T. indicum	710:719	T. indicum	710:719	This study presents a comprehensive investigation of the chemical composition of T. indicum, including free sugars, fatty acids, organic acids, phenolic acids, flavonoids, and polysaccharides, and tracks the antioxidant activity of T. indicum ascocarps collected from five geographical regions of four provinces in P. R. China: Hebei, Tibet, Yunnan, and Liaoning province.
30605248	4	20	theme	ABTS	1227:1230	arg1	activities					1251:1260	ABTS radical-scavenging activities	1227:1260	ABTS radical-scavenging activities	1227:1260	Our results showed that T. indicum collected from Qujing, Yunnan province, possessed the highest amount of free sugars (23.67 mg/g dw), total flavonoids (2.31 mg/g dw), total phenolics (4.46 mg/g dw) and the highest DPPH and ABTS radical-scavenging activities.
30605248	1	21	from	species	143:149	arg1	Asian					162:166	Asian	162:166	Asian	162:166	Tuber indicum, an endemic truffle species in eastern Asian, is an edible mushroom that is both an important export and widely distributed across China.
30605248	7	22	theme	important	1693:1701	arg1	data					1703:1706	important data	1693:1706	important data for understanding the relationship between environmental factors and truffle formation, as well as quality evaluation of the commercial species T. indicum throughout China	1693:1878	Detecting differences in chemical composition may provide important data for understanding the relationship between environmental factors and truffle formation, as well as quality evaluation of the commercial species T. indicum throughout China.
30605248	2	23	theme	pharmacological	497:511	arg1	activity					513:520	pharmacological activity	497:520	pharmacological activity	497:520	Many existing studies on truffles focus on analyzing their taxonomy, population genetics, volatile organic compounds and artificial cultivation of the truffles, while little information is available about their nutrient composition and pharmacological activity, especially the relationship between chemical composition in ascocarps and their geographic distributions.
30605248	6	24	theme	compounds	1580:1588	arg1	quantity					1559:1566	the quantity	1555:1566	the quantity of chemical compounds in T. indicum	1555:1602	This study reveals that the quantity of chemical compounds in T. indicum varies by geographical origin.
30605248	3	25	theme	geographical	902:913	arg1	regions					915:921	five geographical regions	897:921	five geographical regions of four provinces in P. R. China	897:954	This study presents a comprehensive investigation of the chemical composition of T. indicum, including free sugars, fatty acids, organic acids, phenolic acids, flavonoids, and polysaccharides, and tracks the antioxidant activity of T. indicum ascocarps collected from five geographical regions of four provinces in P. R. China: Hebei, Tibet, Yunnan, and Liaoning province.
30605248	2	26	theme	little	428:433	arg1	information					435:445	little information	428:445	little information	428:445	Many existing studies on truffles focus on analyzing their taxonomy, population genetics, volatile organic compounds and artificial cultivation of the truffles, while little information is available about their nutrient composition and pharmacological activity, especially the relationship between chemical composition in ascocarps and their geographic distributions.
30605248	5	27	theme	total	1369:1373	arg1	acids					1383:1387	the total organic acids	1365:1387	the total organic acids	1365:1387	The amount of water-soluble polysaccharides was the highest (115.24 mg/g dw) in ascocarps from Tibet, the total organic acids was the highest (22.073 mg/g dw) in ascocarps from Gongshan, and polyunsaturated fatty acids were most abundant in those from Hebei province.
30605248	5	27	theme	total	1369:1373	arg1	highest					1397:1403	highest	1397:1403	highest	1397:1403	The amount of water-soluble polysaccharides was the highest (115.24 mg/g dw) in ascocarps from Tibet, the total organic acids was the highest (22.073 mg/g dw) in ascocarps from Gongshan, and polyunsaturated fatty acids were most abundant in those from Hebei province.
30605248	4	28	theme	free	1109:1112	arg1	flavonoids					1144:1153	total flavonoids	1138:1153	total flavonoids (2.31 mg/g dw)	1138:1168	Our results showed that T. indicum collected from Qujing, Yunnan province, possessed the highest amount of free sugars (23.67 mg/g dw), total flavonoids (2.31 mg/g dw), total phenolics (4.46 mg/g dw) and the highest DPPH and ABTS radical-scavenging activities.
30605248	4	28	theme	free	1109:1112	arg1	phenolics					1177:1185	total phenolics	1171:1185	total phenolics (4.46 mg/g dw)	1171:1200	Our results showed that T. indicum collected from Qujing, Yunnan province, possessed the highest amount of free sugars (23.67 mg/g dw), total flavonoids (2.31 mg/g dw), total phenolics (4.46 mg/g dw) and the highest DPPH and ABTS radical-scavenging activities.
30605248	4	28	theme	free	1109:1112	arg1	23.67 mg/g dw					1122:1134	23.67 mg/g dw	1122:1134	23.67 mg/g dw	1122:1134	Our results showed that T. indicum collected from Qujing, Yunnan province, possessed the highest amount of free sugars (23.67 mg/g dw), total flavonoids (2.31 mg/g dw), total phenolics (4.46 mg/g dw) and the highest DPPH and ABTS radical-scavenging activities.
30605248	4	28	theme	free	1109:1112	arg1	sugars					1114:1119	free sugars	1109:1119	free sugars (23.67 mg/g dw)	1109:1135	Our results showed that T. indicum collected from Qujing, Yunnan province, possessed the highest amount of free sugars (23.67 mg/g dw), total flavonoids (2.31 mg/g dw), total phenolics (4.46 mg/g dw) and the highest DPPH and ABTS radical-scavenging activities.
30605248	2	29	theme	organic	360:366	arg1	compounds					368:376	volatile organic compounds	351:376	volatile organic compounds	351:376	Many existing studies on truffles focus on analyzing their taxonomy, population genetics, volatile organic compounds and artificial cultivation of the truffles, while little information is available about their nutrient composition and pharmacological activity, especially the relationship between chemical composition in ascocarps and their geographic distributions.
30605248	0	30	theme	Chemical	0:7	arg1	Composition					9:19	Chemical Composition	0:19	Chemical Composition	0:19	Chemical Composition and Antioxidant Activity of Tuber indicum from Different Geographical Regions of China.
30605248	5	31	from	Gongshan	1440:1447	arg1	ascocarps					1425:1433	ascocarps	1425:1433	ascocarps from Gongshan	1425:1447	The amount of water-soluble polysaccharides was the highest (115.24 mg/g dw) in ascocarps from Tibet, the total organic acids was the highest (22.073 mg/g dw) in ascocarps from Gongshan, and polyunsaturated fatty acids were most abundant in those from Hebei province.
30605248	5	32	theme	organic	1375:1381	arg1	acids					1383:1387	the total organic acids	1365:1387	the total organic acids	1365:1387	The amount of water-soluble polysaccharides was the highest (115.24 mg/g dw) in ascocarps from Tibet, the total organic acids was the highest (22.073 mg/g dw) in ascocarps from Gongshan, and polyunsaturated fatty acids were most abundant in those from Hebei province.
30605248	5	32	theme	organic	1375:1381	arg1	highest					1397:1403	highest	1397:1403	highest	1397:1403	The amount of water-soluble polysaccharides was the highest (115.24 mg/g dw) in ascocarps from Tibet, the total organic acids was the highest (22.073 mg/g dw) in ascocarps from Gongshan, and polyunsaturated fatty acids were most abundant in those from Hebei province.
30605248	2	33	theme	volatile	351:358	arg1	compounds					368:376	volatile organic compounds	351:376	volatile organic compounds	351:376	Many existing studies on truffles focus on analyzing their taxonomy, population genetics, volatile organic compounds and artificial cultivation of the truffles, while little information is available about their nutrient composition and pharmacological activity, especially the relationship between chemical composition in ascocarps and their geographic distributions.
30605248	0	34	theme	Antioxidant	25:35	arg1	Activity					37:44	Antioxidant Activity	25:44	Antioxidant Activity	25:44	Chemical Composition and Antioxidant Activity of Tuber indicum from Different Geographical Regions of China.
30605248	2	35	theme	chemical	559:566	arg1	composition					568:578	chemical composition	559:578	chemical composition in ascocarps and their geographic distributions	559:626	Many existing studies on truffles focus on analyzing their taxonomy, population genetics, volatile organic compounds and artificial cultivation of the truffles, while little information is available about their nutrient composition and pharmacological activity, especially the relationship between chemical composition in ascocarps and their geographic distributions.
30605248	3	36	theme	Liaoning	983:990	arg1	province					992:999	Liaoning province	983:999	Liaoning province	983:999	This study presents a comprehensive investigation of the chemical composition of T. indicum, including free sugars, fatty acids, organic acids, phenolic acids, flavonoids, and polysaccharides, and tracks the antioxidant activity of T. indicum ascocarps collected from five geographical regions of four provinces in P. R. China: Hebei, Tibet, Yunnan, and Liaoning province.
30605248	3	36	theme	Liaoning	983:990	arg1	sugars					737:742	free sugars	732:742	free sugars	732:742	This study presents a comprehensive investigation of the chemical composition of T. indicum, including free sugars, fatty acids, organic acids, phenolic acids, flavonoids, and polysaccharides, and tracks the antioxidant activity of T. indicum ascocarps collected from five geographical regions of four provinces in P. R. China: Hebei, Tibet, Yunnan, and Liaoning province.
30605248	1	37	theme	edible	175:180	arg1	mushroom					182:189	an edible mushroom	172:189	an edible mushroom that is both an important export and widely distributed across China	172:258	Tuber indicum, an endemic truffle species in eastern Asian, is an edible mushroom that is both an important export and widely distributed across China.
30605248	1	37	theme	edible	175:180	arg1	indicum					115:121	Tuber indicum	109:121	Tuber indicum	109:121	Tuber indicum, an endemic truffle species in eastern Asian, is an edible mushroom that is both an important export and widely distributed across China.
30605248	2	38	theme	truffles	412:419	arg1	compounds					368:376	volatile organic compounds	351:376	volatile organic compounds	351:376	Many existing studies on truffles focus on analyzing their taxonomy, population genetics, volatile organic compounds and artificial cultivation of the truffles, while little information is available about their nutrient composition and pharmacological activity, especially the relationship between chemical composition in ascocarps and their geographic distributions.
30605248	2	38	theme	truffles	412:419	arg1	cultivation					393:403	artificial cultivation	382:403	artificial cultivation	382:403	Many existing studies on truffles focus on analyzing their taxonomy, population genetics, volatile organic compounds and artificial cultivation of the truffles, while little information is available about their nutrient composition and pharmacological activity, especially the relationship between chemical composition in ascocarps and their geographic distributions.
30605248	2	38	theme	truffles	412:419	arg1	taxonomy					320:327	their taxonomy	314:327	their taxonomy	314:327	Many existing studies on truffles focus on analyzing their taxonomy, population genetics, volatile organic compounds and artificial cultivation of the truffles, while little information is available about their nutrient composition and pharmacological activity, especially the relationship between chemical composition in ascocarps and their geographic distributions.
30605248	2	38	theme	truffles	412:419	arg1	genetics					341:348	population genetics	330:348	population genetics	330:348	Many existing studies on truffles focus on analyzing their taxonomy, population genetics, volatile organic compounds and artificial cultivation of the truffles, while little information is available about their nutrient composition and pharmacological activity, especially the relationship between chemical composition in ascocarps and their geographic distributions.
30605248	0	39	from	Regions	91:97	arg1	Composition					9:19	Chemical Composition	0:19	Chemical Composition	0:19	Chemical Composition and Antioxidant Activity of Tuber indicum from Different Geographical Regions of China.
30605248	0	39	from	Regions	91:97	arg1	Activity					37:44	Antioxidant Activity	25:44	Antioxidant Activity	25:44	Chemical Composition and Antioxidant Activity of Tuber indicum from Different Geographical Regions of China.
30605248	1	40	theme	Tuber	109:113	arg1	species					143:149	an endemic truffle species	124:149	an endemic truffle species in eastern Asian	124:166	Tuber indicum, an endemic truffle species in eastern Asian, is an edible mushroom that is both an important export and widely distributed across China.
30605248	1	40	theme	Tuber	109:113	arg1	indicum					115:121	Tuber indicum	109:121	Tuber indicum	109:121	Tuber indicum, an endemic truffle species in eastern Asian, is an edible mushroom that is both an important export and widely distributed across China.
30605248	1	40	theme	Tuber	109:113	arg1	mushroom					182:189	an edible mushroom	172:189	an edible mushroom that is both an important export and widely distributed across China	172:258	Tuber indicum, an endemic truffle species in eastern Asian, is an edible mushroom that is both an important export and widely distributed across China.
30605248	3	41	theme	provinces	931:939	arg1	regions					915:921	five geographical regions	897:921	five geographical regions of four provinces in P. R. China	897:954	This study presents a comprehensive investigation of the chemical composition of T. indicum, including free sugars, fatty acids, organic acids, phenolic acids, flavonoids, and polysaccharides, and tracks the antioxidant activity of T. indicum ascocarps collected from five geographical regions of four provinces in P. R. China: Hebei, Tibet, Yunnan, and Liaoning province.
30605248	3	42	theme	organic	758:764	arg1	acids					766:770	organic acids	758:770	organic acids	758:770	This study presents a comprehensive investigation of the chemical composition of T. indicum, including free sugars, fatty acids, organic acids, phenolic acids, flavonoids, and polysaccharides, and tracks the antioxidant activity of T. indicum ascocarps collected from five geographical regions of four provinces in P. R. China: Hebei, Tibet, Yunnan, and Liaoning province.
30605248	1	43	dep	export	217:222	arg1	both					199:202	both	199:202	both	199:202	Tuber indicum, an endemic truffle species in eastern Asian, is an edible mushroom that is both an important export and widely distributed across China.
30605248	3	44	from	provinces	931:939	arg1	China					950:954	China	950:954	China	950:954	This study presents a comprehensive investigation of the chemical composition of T. indicum, including free sugars, fatty acids, organic acids, phenolic acids, flavonoids, and polysaccharides, and tracks the antioxidant activity of T. indicum ascocarps collected from five geographical regions of four provinces in P. R. China: Hebei, Tibet, Yunnan, and Liaoning province.
30605248	5	45	theme	water-soluble	1277:1289	arg1	polysaccharides					1291:1305	water-soluble polysaccharides	1277:1305	water-soluble polysaccharides	1277:1305	The amount of water-soluble polysaccharides was the highest (115.24 mg/g dw) in ascocarps from Tibet, the total organic acids was the highest (22.073 mg/g dw) in ascocarps from Gongshan, and polyunsaturated fatty acids were most abundant in those from Hebei province.
30605248	5	46	from	Tibet	1358:1362	arg1	ascocarps					1343:1351	ascocarps	1343:1351	ascocarps from Tibet	1343:1362	The amount of water-soluble polysaccharides was the highest (115.24 mg/g dw) in ascocarps from Tibet, the total organic acids was the highest (22.073 mg/g dw) in ascocarps from Gongshan, and polyunsaturated fatty acids were most abundant in those from Hebei province.
30605248	7	47	theme	environmental	1751:1763	arg1	factors					1765:1771	environmental factors	1751:1771	environmental factors	1751:1771	Detecting differences in chemical composition may provide important data for understanding the relationship between environmental factors and truffle formation, as well as quality evaluation of the commercial species T. indicum throughout China.
30605248	3	48	dep	sugars	737:742	arg1	province					992:999	Liaoning province	983:999	Liaoning province	983:999	This study presents a comprehensive investigation of the chemical composition of T. indicum, including free sugars, fatty acids, organic acids, phenolic acids, flavonoids, and polysaccharides, and tracks the antioxidant activity of T. indicum ascocarps collected from five geographical regions of four provinces in P. R. China: Hebei, Tibet, Yunnan, and Liaoning province.
30605248	3	48	dep	sugars	737:742	arg1	activity					849:856	the antioxidant activity	833:856	the antioxidant activity of T. indicum ascocarps collected from five geographical regions of four provinces in P. R. China	833:954	This study presents a comprehensive investigation of the chemical composition of T. indicum, including free sugars, fatty acids, organic acids, phenolic acids, flavonoids, and polysaccharides, and tracks the antioxidant activity of T. indicum ascocarps collected from five geographical regions of four provinces in P. R. China: Hebei, Tibet, Yunnan, and Liaoning province.
30605248	3	48	dep	sugars	737:742	arg1	Tibet					964:968	Tibet	964:968	Tibet	964:968	This study presents a comprehensive investigation of the chemical composition of T. indicum, including free sugars, fatty acids, organic acids, phenolic acids, flavonoids, and polysaccharides, and tracks the antioxidant activity of T. indicum ascocarps collected from five geographical regions of four provinces in P. R. China: Hebei, Tibet, Yunnan, and Liaoning province.
30605248	3	48	dep	sugars	737:742	arg1	Yunnan					971:976	Yunnan	971:976	Yunnan	971:976	This study presents a comprehensive investigation of the chemical composition of T. indicum, including free sugars, fatty acids, organic acids, phenolic acids, flavonoids, and polysaccharides, and tracks the antioxidant activity of T. indicum ascocarps collected from five geographical regions of four provinces in P. R. China: Hebei, Tibet, Yunnan, and Liaoning province.
30605248	3	48	dep	sugars	737:742	arg1	Hebei					957:961	Hebei	957:961	Hebei	957:961	This study presents a comprehensive investigation of the chemical composition of T. indicum, including free sugars, fatty acids, organic acids, phenolic acids, flavonoids, and polysaccharides, and tracks the antioxidant activity of T. indicum ascocarps collected from five geographical regions of four provinces in P. R. China: Hebei, Tibet, Yunnan, and Liaoning province.
30605248	3	48	dep	sugars	737:742	arg1	sugars					737:742	free sugars	732:742	free sugars	732:742	This study presents a comprehensive investigation of the chemical composition of T. indicum, including free sugars, fatty acids, organic acids, phenolic acids, flavonoids, and polysaccharides, and tracks the antioxidant activity of T. indicum ascocarps collected from five geographical regions of four provinces in P. R. China: Hebei, Tibet, Yunnan, and Liaoning province.
30605248	3	49	theme	antioxidant	837:847	arg1	activity					849:856	the antioxidant activity	833:856	the antioxidant activity of T. indicum ascocarps collected from five geographical regions of four provinces in P. R. China	833:954	This study presents a comprehensive investigation of the chemical composition of T. indicum, including free sugars, fatty acids, organic acids, phenolic acids, flavonoids, and polysaccharides, and tracks the antioxidant activity of T. indicum ascocarps collected from five geographical regions of four provinces in P. R. China: Hebei, Tibet, Yunnan, and Liaoning province.
30605248	3	49	theme	antioxidant	837:847	arg1	sugars					737:742	free sugars	732:742	free sugars	732:742	This study presents a comprehensive investigation of the chemical composition of T. indicum, including free sugars, fatty acids, organic acids, phenolic acids, flavonoids, and polysaccharides, and tracks the antioxidant activity of T. indicum ascocarps collected from five geographical regions of four provinces in P. R. China: Hebei, Tibet, Yunnan, and Liaoning province.
30605248	3	50	theme	comprehensive	651:663	arg1	investigation					665:677	a comprehensive investigation	649:677	a comprehensive investigation of the chemical composition of T. indicum	649:719	This study presents a comprehensive investigation of the chemical composition of T. indicum, including free sugars, fatty acids, organic acids, phenolic acids, flavonoids, and polysaccharides, and tracks the antioxidant activity of T. indicum ascocarps collected from five geographical regions of four provinces in P. R. China: Hebei, Tibet, Yunnan, and Liaoning province.
30605248	2	51	theme	artificial	382:391	arg1	cultivation					393:403	artificial cultivation	382:403	artificial cultivation	382:403	Many existing studies on truffles focus on analyzing their taxonomy, population genetics, volatile organic compounds and artificial cultivation of the truffles, while little information is available about their nutrient composition and pharmacological activity, especially the relationship between chemical composition in ascocarps and their geographic distributions.
30605248	0	52	theme	indicum	55:61	arg1	Composition					9:19	Chemical Composition	0:19	Chemical Composition	0:19	Chemical Composition and Antioxidant Activity of Tuber indicum from Different Geographical Regions of China.
30605248	0	52	theme	indicum	55:61	arg1	Activity					37:44	Antioxidant Activity	25:44	Antioxidant Activity	25:44	Chemical Composition and Antioxidant Activity of Tuber indicum from Different Geographical Regions of China.
30605248	5	53	theme	polyunsaturated	1454:1468	arg1	acids					1476:1480	polyunsaturated fatty acids	1454:1480	polyunsaturated fatty acids	1454:1480	The amount of water-soluble polysaccharides was the highest (115.24 mg/g dw) in ascocarps from Tibet, the total organic acids was the highest (22.073 mg/g dw) in ascocarps from Gongshan, and polyunsaturated fatty acids were most abundant in those from Hebei province.
30605248	4	54	theme	highest	1091:1097	arg1	flavonoids					1144:1153	total flavonoids	1138:1153	total flavonoids (2.31 mg/g dw)	1138:1168	Our results showed that T. indicum collected from Qujing, Yunnan province, possessed the highest amount of free sugars (23.67 mg/g dw), total flavonoids (2.31 mg/g dw), total phenolics (4.46 mg/g dw) and the highest DPPH and ABTS radical-scavenging activities.
30605248	4	54	theme	highest	1091:1097	arg1	phenolics					1177:1185	total phenolics	1171:1185	total phenolics (4.46 mg/g dw)	1171:1200	Our results showed that T. indicum collected from Qujing, Yunnan province, possessed the highest amount of free sugars (23.67 mg/g dw), total flavonoids (2.31 mg/g dw), total phenolics (4.46 mg/g dw) and the highest DPPH and ABTS radical-scavenging activities.
30605248	4	54	theme	highest	1091:1097	arg1	23.67 mg/g dw					1122:1134	23.67 mg/g dw	1122:1134	23.67 mg/g dw	1122:1134	Our results showed that T. indicum collected from Qujing, Yunnan province, possessed the highest amount of free sugars (23.67 mg/g dw), total flavonoids (2.31 mg/g dw), total phenolics (4.46 mg/g dw) and the highest DPPH and ABTS radical-scavenging activities.
30605248	4	54	theme	highest	1091:1097	arg1	amount					1099:1104	the highest amount	1087:1104	the highest amount of free sugars (23.67 mg/g dw), total flavonoids (2.31 mg/g dw), total phenolics (4.46 mg/g dw)	1087:1200	Our results showed that T. indicum collected from Qujing, Yunnan province, possessed the highest amount of free sugars (23.67 mg/g dw), total flavonoids (2.31 mg/g dw), total phenolics (4.46 mg/g dw) and the highest DPPH and ABTS radical-scavenging activities.
30605248	4	54	theme	highest	1091:1097	arg1	sugars					1114:1119	free sugars	1109:1119	free sugars (23.67 mg/g dw)	1109:1135	Our results showed that T. indicum collected from Qujing, Yunnan province, possessed the highest amount of free sugars (23.67 mg/g dw), total flavonoids (2.31 mg/g dw), total phenolics (4.46 mg/g dw) and the highest DPPH and ABTS radical-scavenging activities.
30605248	4	55	theme	total	1138:1142	arg1	flavonoids					1144:1153	total flavonoids	1138:1153	total flavonoids (2.31 mg/g dw)	1138:1168	Our results showed that T. indicum collected from Qujing, Yunnan province, possessed the highest amount of free sugars (23.67 mg/g dw), total flavonoids (2.31 mg/g dw), total phenolics (4.46 mg/g dw) and the highest DPPH and ABTS radical-scavenging activities.
30605248	4	55	theme	total	1138:1142	arg1	sugars					1114:1119	free sugars	1109:1119	free sugars (23.67 mg/g dw)	1109:1135	Our results showed that T. indicum collected from Qujing, Yunnan province, possessed the highest amount of free sugars (23.67 mg/g dw), total flavonoids (2.31 mg/g dw), total phenolics (4.46 mg/g dw) and the highest DPPH and ABTS radical-scavenging activities.
30605248	4	55	theme	total	1138:1142	arg1	2.31 mg/g dw					1156:1167	2.31 mg/g dw	1156:1167	2.31 mg/g dw	1156:1167	Our results showed that T. indicum collected from Qujing, Yunnan province, possessed the highest amount of free sugars (23.67 mg/g dw), total flavonoids (2.31 mg/g dw), total phenolics (4.46 mg/g dw) and the highest DPPH and ABTS radical-scavenging activities.
30605248	6	56	from	quantity	1559:1566	arg1	indicum					1596:1602	T. indicum	1593:1602	T. indicum	1593:1602	This study reveals that the quantity of chemical compounds in T. indicum varies by geographical origin.
30605248	0	57	theme	Tuber	49:53	arg1	indicum					55:61	Tuber indicum	49:61	Tuber indicum	49:61	Chemical Composition and Antioxidant Activity of Tuber indicum from Different Geographical Regions of China.
30605248	3	58	theme	phenolic	773:780	arg1	acids					782:786	phenolic acids	773:786	phenolic acids	773:786	This study presents a comprehensive investigation of the chemical composition of T. indicum, including free sugars, fatty acids, organic acids, phenolic acids, flavonoids, and polysaccharides, and tracks the antioxidant activity of T. indicum ascocarps collected from five geographical regions of four provinces in P. R. China: Hebei, Tibet, Yunnan, and Liaoning province.
30605248	5	59	theme	fatty	1470:1474	arg1	acids					1476:1480	polyunsaturated fatty acids	1454:1480	polyunsaturated fatty acids	1454:1480	The amount of water-soluble polysaccharides was the highest (115.24 mg/g dw) in ascocarps from Tibet, the total organic acids was the highest (22.073 mg/g dw) in ascocarps from Gongshan, and polyunsaturated fatty acids were most abundant in those from Hebei province.
30605248	4	60	theme	highest	1210:1216	arg1	DPPH					1218:1221	the highest DPPH	1206:1221	the highest DPPH	1206:1221	Our results showed that T. indicum collected from Qujing, Yunnan province, possessed the highest amount of free sugars (23.67 mg/g dw), total flavonoids (2.31 mg/g dw), total phenolics (4.46 mg/g dw) and the highest DPPH and ABTS radical-scavenging activities.
30605248	6	61	theme	geographical	1614:1625	arg1	origin					1627:1632	geographical origin	1614:1632	geographical origin	1614:1632	This study reveals that the quantity of chemical compounds in T. indicum varies by geographical origin.
30605248	7	62	theme	quality	1807:1813	arg1	evaluation					1815:1824	quality evaluation	1807:1824	quality evaluation of the commercial species	1807:1850	Detecting differences in chemical composition may provide important data for understanding the relationship between environmental factors and truffle formation, as well as quality evaluation of the commercial species T. indicum throughout China.
30605248	5	63	from	abundant	1492:1499	arg1	those					1504:1508	those	1504:1508	those	1504:1508	The amount of water-soluble polysaccharides was the highest (115.24 mg/g dw) in ascocarps from Tibet, the total organic acids was the highest (22.073 mg/g dw) in ascocarps from Gongshan, and polyunsaturated fatty acids were most abundant in those from Hebei province.
30605248	3	64	theme	T.	861:862	arg1	ascocarps					872:880	T. indicum ascocarps	861:880	T. indicum ascocarps collected from five geographical regions of four provinces in P. R. China	861:954	This study presents a comprehensive investigation of the chemical composition of T. indicum, including free sugars, fatty acids, organic acids, phenolic acids, flavonoids, and polysaccharides, and tracks the antioxidant activity of T. indicum ascocarps collected from five geographical regions of four provinces in P. R. China: Hebei, Tibet, Yunnan, and Liaoning province.
30605248	4	65	theme	total	1171:1175	arg1	4.46 mg/g dw					1188:1199	4.46 mg/g dw	1188:1199	4.46 mg/g dw	1188:1199	Our results showed that T. indicum collected from Qujing, Yunnan province, possessed the highest amount of free sugars (23.67 mg/g dw), total flavonoids (2.31 mg/g dw), total phenolics (4.46 mg/g dw) and the highest DPPH and ABTS radical-scavenging activities.
30605248	4	65	theme	total	1171:1175	arg1	phenolics					1177:1185	total phenolics	1171:1185	total phenolics (4.46 mg/g dw)	1171:1200	Our results showed that T. indicum collected from Qujing, Yunnan province, possessed the highest amount of free sugars (23.67 mg/g dw), total flavonoids (2.31 mg/g dw), total phenolics (4.46 mg/g dw) and the highest DPPH and ABTS radical-scavenging activities.
30605248	4	65	theme	total	1171:1175	arg1	sugars					1114:1119	free sugars	1109:1119	free sugars (23.67 mg/g dw)	1109:1135	Our results showed that T. indicum collected from Qujing, Yunnan province, possessed the highest amount of free sugars (23.67 mg/g dw), total flavonoids (2.31 mg/g dw), total phenolics (4.46 mg/g dw) and the highest DPPH and ABTS radical-scavenging activities.
30605248	7	66	theme	commercial	1833:1842	arg1	species					1844:1850	the commercial species	1829:1850	the commercial species	1829:1850	Detecting differences in chemical composition may provide important data for understanding the relationship between environmental factors and truffle formation, as well as quality evaluation of the commercial species T. indicum throughout China.
30605248	3	67	theme	T.	710:711	arg1	composition					695:705	the chemical composition	682:705	the chemical composition of T. indicum	682:719	This study presents a comprehensive investigation of the chemical composition of T. indicum, including free sugars, fatty acids, organic acids, phenolic acids, flavonoids, and polysaccharides, and tracks the antioxidant activity of T. indicum ascocarps collected from five geographical regions of four provinces in P. R. China: Hebei, Tibet, Yunnan, and Liaoning province.
30605248	3	68	theme	indicum	864:870	arg1	ascocarps					872:880	T. indicum ascocarps	861:880	T. indicum ascocarps collected from five geographical regions of four provinces in P. R. China	861:954	This study presents a comprehensive investigation of the chemical composition of T. indicum, including free sugars, fatty acids, organic acids, phenolic acids, flavonoids, and polysaccharides, and tracks the antioxidant activity of T. indicum ascocarps collected from five geographical regions of four provinces in P. R. China: Hebei, Tibet, Yunnan, and Liaoning province.
30605248	6	69	theme	chemical	1571:1578	arg1	compounds					1580:1588	chemical compounds	1571:1588	chemical compounds	1571:1588	This study reveals that the quantity of chemical compounds in T. indicum varies by geographical origin.
30605248	3	70	theme	chemical	686:693	arg1	composition					695:705	the chemical composition	682:705	the chemical composition of T. indicum	682:719	This study presents a comprehensive investigation of the chemical composition of T. indicum, including free sugars, fatty acids, organic acids, phenolic acids, flavonoids, and polysaccharides, and tracks the antioxidant activity of T. indicum ascocarps collected from five geographical regions of four provinces in P. R. China: Hebei, Tibet, Yunnan, and Liaoning province.
30605248	3	71	from	regions	915:921	arg1	China					950:954	China	950:954	China	950:954	This study presents a comprehensive investigation of the chemical composition of T. indicum, including free sugars, fatty acids, organic acids, phenolic acids, flavonoids, and polysaccharides, and tracks the antioxidant activity of T. indicum ascocarps collected from five geographical regions of four provinces in P. R. China: Hebei, Tibet, Yunnan, and Liaoning province.
30605248	4	72	contain	possessed	1077:1085	arg1	indicum					1029:1035	T. indicum	1026:1035	T. indicum	1026:1035	Our results showed that T. indicum collected from Qujing, Yunnan province, possessed the highest amount of free sugars (23.67 mg/g dw), total flavonoids (2.31 mg/g dw), total phenolics (4.46 mg/g dw) and the highest DPPH and ABTS radical-scavenging activities.
30605248	4	72	contain	possessed	1077:1085	arg2	23.67 mg/g dw					1122:1134	23.67 mg/g dw	1122:1134	23.67 mg/g dw	1122:1134	Our results showed that T. indicum collected from Qujing, Yunnan province, possessed the highest amount of free sugars (23.67 mg/g dw), total flavonoids (2.31 mg/g dw), total phenolics (4.46 mg/g dw) and the highest DPPH and ABTS radical-scavenging activities.
30605248	4	72	contain	possessed	1077:1085	arg2	amount					1099:1104	the highest amount	1087:1104	the highest amount of free sugars (23.67 mg/g dw), total flavonoids (2.31 mg/g dw), total phenolics (4.46 mg/g dw)	1087:1200	Our results showed that T. indicum collected from Qujing, Yunnan province, possessed the highest amount of free sugars (23.67 mg/g dw), total flavonoids (2.31 mg/g dw), total phenolics (4.46 mg/g dw) and the highest DPPH and ABTS radical-scavenging activities.
30605248	4	72	contain	possessed	1077:1085	arg2	sugars					1114:1119	free sugars	1109:1119	free sugars (23.67 mg/g dw)	1109:1135	Our results showed that T. indicum collected from Qujing, Yunnan province, possessed the highest amount of free sugars (23.67 mg/g dw), total flavonoids (2.31 mg/g dw), total phenolics (4.46 mg/g dw) and the highest DPPH and ABTS radical-scavenging activities.
30605248	4	72	contain	possessed	1077:1085	arg2	flavonoids					1144:1153	total flavonoids	1138:1153	total flavonoids (2.31 mg/g dw)	1138:1168	Our results showed that T. indicum collected from Qujing, Yunnan province, possessed the highest amount of free sugars (23.67 mg/g dw), total flavonoids (2.31 mg/g dw), total phenolics (4.46 mg/g dw) and the highest DPPH and ABTS radical-scavenging activities.
30605248	4	72	contain	possessed	1077:1085	arg2	phenolics					1177:1185	total phenolics	1171:1185	total phenolics (4.46 mg/g dw)	1171:1200	Our results showed that T. indicum collected from Qujing, Yunnan province, possessed the highest amount of free sugars (23.67 mg/g dw), total flavonoids (2.31 mg/g dw), total phenolics (4.46 mg/g dw) and the highest DPPH and ABTS radical-scavenging activities.
30605248	4	72	contain	possessed	1077:1085	arg2	DPPH					1218:1221	the highest DPPH	1206:1221	the highest DPPH	1206:1221	Our results showed that T. indicum collected from Qujing, Yunnan province, possessed the highest amount of free sugars (23.67 mg/g dw), total flavonoids (2.31 mg/g dw), total phenolics (4.46 mg/g dw) and the highest DPPH and ABTS radical-scavenging activities.
30605248	1	73	theme	endemic	127:133	arg1	species					143:149	an endemic truffle species	124:149	an endemic truffle species in eastern Asian	124:166	Tuber indicum, an endemic truffle species in eastern Asian, is an edible mushroom that is both an important export and widely distributed across China.
30605248	1	73	theme	endemic	127:133	arg1	indicum					115:121	Tuber indicum	109:121	Tuber indicum	109:121	Tuber indicum, an endemic truffle species in eastern Asian, is an edible mushroom that is both an important export and widely distributed across China.
30605248	4	74	theme	radical-scavenging	1232:1249	arg1	activities					1251:1260	ABTS radical-scavenging activities	1227:1260	ABTS radical-scavenging activities	1227:1260	Our results showed that T. indicum collected from Qujing, Yunnan province, possessed the highest amount of free sugars (23.67 mg/g dw), total flavonoids (2.31 mg/g dw), total phenolics (4.46 mg/g dw) and the highest DPPH and ABTS radical-scavenging activities.
30605248	4	75	theme	sugars	1114:1119	arg1	phenolics					1177:1185	total phenolics	1171:1185	total phenolics (4.46 mg/g dw)	1171:1200	Our results showed that T. indicum collected from Qujing, Yunnan province, possessed the highest amount of free sugars (23.67 mg/g dw), total flavonoids (2.31 mg/g dw), total phenolics (4.46 mg/g dw) and the highest DPPH and ABTS radical-scavenging activities.
30605248	4	75	theme	sugars	1114:1119	arg1	sugars					1114:1119	free sugars	1109:1119	free sugars (23.67 mg/g dw)	1109:1135	Our results showed that T. indicum collected from Qujing, Yunnan province, possessed the highest amount of free sugars (23.67 mg/g dw), total flavonoids (2.31 mg/g dw), total phenolics (4.46 mg/g dw) and the highest DPPH and ABTS radical-scavenging activities.
30605248	4	75	theme	sugars	1114:1119	arg1	DPPH					1218:1221	the highest DPPH	1206:1221	the highest DPPH	1206:1221	Our results showed that T. indicum collected from Qujing, Yunnan province, possessed the highest amount of free sugars (23.67 mg/g dw), total flavonoids (2.31 mg/g dw), total phenolics (4.46 mg/g dw) and the highest DPPH and ABTS radical-scavenging activities.
30605248	4	75	theme	sugars	1114:1119	arg1	flavonoids					1144:1153	total flavonoids	1138:1153	total flavonoids (2.31 mg/g dw)	1138:1168	Our results showed that T. indicum collected from Qujing, Yunnan province, possessed the highest amount of free sugars (23.67 mg/g dw), total flavonoids (2.31 mg/g dw), total phenolics (4.46 mg/g dw) and the highest DPPH and ABTS radical-scavenging activities.
30605248	4	75	theme	sugars	1114:1119	arg1	23.67 mg/g dw					1122:1134	23.67 mg/g dw	1122:1134	23.67 mg/g dw	1122:1134	Our results showed that T. indicum collected from Qujing, Yunnan province, possessed the highest amount of free sugars (23.67 mg/g dw), total flavonoids (2.31 mg/g dw), total phenolics (4.46 mg/g dw) and the highest DPPH and ABTS radical-scavenging activities.
30605248	4	75	theme	sugars	1114:1119	arg1	activities					1251:1260	ABTS radical-scavenging activities	1227:1260	ABTS radical-scavenging activities	1227:1260	Our results showed that T. indicum collected from Qujing, Yunnan province, possessed the highest amount of free sugars (23.67 mg/g dw), total flavonoids (2.31 mg/g dw), total phenolics (4.46 mg/g dw) and the highest DPPH and ABTS radical-scavenging activities.
30605248	4	75	theme	sugars	1114:1119	arg1	amount					1099:1104	the highest amount	1087:1104	the highest amount of free sugars (23.67 mg/g dw), total flavonoids (2.31 mg/g dw), total phenolics (4.46 mg/g dw)	1087:1200	Our results showed that T. indicum collected from Qujing, Yunnan province, possessed the highest amount of free sugars (23.67 mg/g dw), total flavonoids (2.31 mg/g dw), total phenolics (4.46 mg/g dw) and the highest DPPH and ABTS radical-scavenging activities.
30605248	0	76	theme	Different	68:76	arg1	Regions					91:97	Different Geographical Regions	68:97	Different Geographical Regions of China	68:106	Chemical Composition and Antioxidant Activity of Tuber indicum from Different Geographical Regions of China.
30605248	3	77	theme	composition	695:705	arg1	investigation					665:677	a comprehensive investigation	649:677	a comprehensive investigation of the chemical composition of T. indicum	649:719	This study presents a comprehensive investigation of the chemical composition of T. indicum, including free sugars, fatty acids, organic acids, phenolic acids, flavonoids, and polysaccharides, and tracks the antioxidant activity of T. indicum ascocarps collected from five geographical regions of four provinces in P. R. China: Hebei, Tibet, Yunnan, and Liaoning province.
30605248	7	78	from	differences	1645:1655	arg1	composition					1669:1679	chemical composition	1660:1679	chemical composition	1660:1679	Detecting differences in chemical composition may provide important data for understanding the relationship between environmental factors and truffle formation, as well as quality evaluation of the commercial species T. indicum throughout China.
30605248	3	79	from	China	950:954	arg1	regions					915:921	five geographical regions	897:921	five geographical regions of four provinces in P. R. China	897:954	This study presents a comprehensive investigation of the chemical composition of T. indicum, including free sugars, fatty acids, organic acids, phenolic acids, flavonoids, and polysaccharides, and tracks the antioxidant activity of T. indicum ascocarps collected from five geographical regions of four provinces in P. R. China: Hebei, Tibet, Yunnan, and Liaoning province.
30605248	1	80	theme	truffle	135:141	arg1	species					143:149	an endemic truffle species	124:149	an endemic truffle species in eastern Asian	124:166	Tuber indicum, an endemic truffle species in eastern Asian, is an edible mushroom that is both an important export and widely distributed across China.
30605248	1	80	theme	truffle	135:141	arg1	indicum					115:121	Tuber indicum	109:121	Tuber indicum	109:121	Tuber indicum, an endemic truffle species in eastern Asian, is an edible mushroom that is both an important export and widely distributed across China.
30605248	1	81	theme	important	207:215	arg1	export					217:222	both an important export	199:222	export	217:222	Tuber indicum, an endemic truffle species in eastern Asian, is an edible mushroom that is both an important export and widely distributed across China.
31556231	3	0	from	microbiota	428:437	arg1	spondylitis					453:463	ankylosing spondylitis	442:463	ankylosing spondylitis	442:463	However, little is known about the effect of TNF-α blocker treatment on the gut microbiota in ankylosing spondylitis.
31556231	6	1	from	incidence	725:733	arg1	mice					789:792	the proteoglycan-induced mice	764:792	the proteoglycan-induced mice	764:792	rhTNFR:Fc treatment attenuated the arthritis incidence and severity of arthritis in the proteoglycan-induced mice and decreased inflammation in the ankle joints and ameliorated ileal tissue destruction.
31556231	7	2	theme	high	893:896	arg1	permeability					902:913	high gut permeability	893:913	high gut permeability	893:913	Moreover, high gut permeability occurred, and zonula occludens-1 and occludin protein levels were reduced in proteoglycan-induced mice.
31556231	11	3	theme	proteoglycan-induced	1387:1406	arg1	progression					1418:1428	proteoglycan-induced arthritis progression	1387:1428	proteoglycan-induced arthritis progression	1387:1428	In conclusion, anti-TNF-α therapy attenuated proteoglycan-induced arthritis progression and modulated the gut microbiota and intestinal barrier function.
31556231	4	4	theme	blocker	496:502	arg1	effect					478:483	the effect	474:483	the effect of a TNF-α blocker on the gut microbiota in proteoglycan-induced arthritis	474:558	Herein, the effect of a TNF-α blocker on the gut microbiota in proteoglycan-induced arthritis was investigated.
31556231	5	5	theme	etanercept	647:656	arg1	solution					635:642	an rhTNFR:Fc solution	622:642	an rhTNFR:Fc solution of etanercept (5 µg/g) for 4 weeks	622:677	Proteoglycan-induced mice were treated with an rhTNFR:Fc solution of etanercept (5 µg/g) for 4 weeks.
31556231	6	6	theme	ileal	857:861	arg1	destruction					870:880	ameliorated ileal tissue destruction	845:880	ameliorated ileal tissue destruction	845:880	rhTNFR:Fc treatment attenuated the arthritis incidence and severity of arthritis in the proteoglycan-induced mice and decreased inflammation in the ankle joints and ameliorated ileal tissue destruction.
31556231	4	7	theme	TNF-α	490:494	arg1	blocker					496:502	a TNF-α blocker	488:502	a TNF-α blocker	488:502	Herein, the effect of a TNF-α blocker on the gut microbiota in proteoglycan-induced arthritis was investigated.
31556231	5	8	theme	rhTNFR	625:630	arg1	solution					635:642	an rhTNFR:Fc solution	622:642	an rhTNFR:Fc solution of etanercept (5 µg/g) for 4 weeks	622:677	Proteoglycan-induced mice were treated with an rhTNFR:Fc solution of etanercept (5 µg/g) for 4 weeks.
31556231	11	9	theme	gut	1448:1450	arg1	microbiota					1452:1461	the gut microbiota and intestinal barrier function	1444:1493	microbiota	1452:1461	In conclusion, anti-TNF-α therapy attenuated proteoglycan-induced arthritis progression and modulated the gut microbiota and intestinal barrier function.
31556231	7	10	theme	occludin	952:959	arg1	levels					969:974	zonula occludens-1 and occludin protein levels	929:974	zonula occludens-1 and occludin protein levels	929:974	Moreover, high gut permeability occurred, and zonula occludens-1 and occludin protein levels were reduced in proteoglycan-induced mice.
31556231	7	11	theme	gut	898:900	arg1	permeability					902:913	high gut permeability	893:913	high gut permeability	893:913	Moreover, high gut permeability occurred, and zonula occludens-1 and occludin protein levels were reduced in proteoglycan-induced mice.
31556231	10	12	theme	high-throughput	1191:1205	arg1	sequencing					1207:1216	16S rDNA high-throughput sequencing	1182:1216	16S rDNA high-throughput sequencing	1182:1216	In addition, flora analysis via 16S rDNA high-throughput sequencing revealed that rhTNFR:Fc treatment restored the gut microbiota composition to a composition similar to that in control mice.
31556231	11	13	theme	intestinal	1467:1476	arg1	function					1486:1493	the gut microbiota and intestinal barrier function	1444:1493	function	1486:1493	In conclusion, anti-TNF-α therapy attenuated proteoglycan-induced arthritis progression and modulated the gut microbiota and intestinal barrier function.
31556231	4	14	from	effect	478:483	arg1	microbiota					515:524	the gut microbiota	507:524	the gut microbiota in proteoglycan-induced arthritis	507:558	Herein, the effect of a TNF-α blocker on the gut microbiota in proteoglycan-induced arthritis was investigated.
31556231	7	15	theme	protein	961:967	arg1	levels					969:974	zonula occludens-1 and occludin protein levels	929:974	zonula occludens-1 and occludin protein levels	929:974	Moreover, high gut permeability occurred, and zonula occludens-1 and occludin protein levels were reduced in proteoglycan-induced mice.
31556231	2	16	theme	gut	277:279	arg1	microbiota					281:290	the gut microbiota	273:290	the gut microbiota in many diseases, including inflammatory bowel disease	273:345	Anti-tumor necrosis factor-alpha (anti-TNF-α) therapy alters the gut microbiota in many diseases, including inflammatory bowel disease.
31556231	6	17	theme	Fc	687:688	arg1	treatment					690:698	Fc treatment	687:698	Fc treatment	687:698	rhTNFR:Fc treatment attenuated the arthritis incidence and severity of arthritis in the proteoglycan-induced mice and decreased inflammation in the ankle joints and ameliorated ileal tissue destruction.
31556231	3	18	theme	gut	424:426	arg1	microbiota					428:437	the gut microbiota	420:437	the gut microbiota in ankylosing spondylitis	420:463	However, little is known about the effect of TNF-α blocker treatment on the gut microbiota in ankylosing spondylitis.
31556231	12	19	theme	gut	1584:1586	arg1	microbiota					1588:1597	the gut microbiota	1580:1597	the gut microbiota in ankylosing spondylitis	1580:1623	These results provide new insights for anti-TNF-α therapy strategies via regulating the gut microbiota in ankylosing spondylitis.
31556231	0	20	from	microbiota	34:43	arg1	spondylitis					80:90	proteoglycan-induced ankylosing spondylitis	48:90	proteoglycan-induced ankylosing spondylitis in mice	48:98	Anti-TNF-α therapy alters the gut microbiota in proteoglycan-induced ankylosing spondylitis in mice.
31556231	4	21	from	microbiota	515:524	arg1	arthritis					550:558	proteoglycan-induced arthritis	529:558	proteoglycan-induced arthritis	529:558	Herein, the effect of a TNF-α blocker on the gut microbiota in proteoglycan-induced arthritis was investigated.
31556231	7	22	theme	occludens-1	936:946	arg1	levels					969:974	zonula occludens-1 and occludin protein levels	929:974	zonula occludens-1 and occludin protein levels	929:974	Moreover, high gut permeability occurred, and zonula occludens-1 and occludin protein levels were reduced in proteoglycan-induced mice.
31556231	1	23	dep	chronic	129:135	arg1	progressive					138:148	progressive	138:148	progressive	138:148	Ankylosing spondylitis is a chronic, progressive disease, and its treatment is relevant to the gut microbiota.
31556231	3	24	from	effect	383:388	arg1	microbiota					428:437	the gut microbiota	420:437	the gut microbiota in ankylosing spondylitis	420:463	However, little is known about the effect of TNF-α blocker treatment on the gut microbiota in ankylosing spondylitis.
31556231	5	25	theme	Fc	632:633	arg1	solution					635:642	an rhTNFR:Fc solution	622:642	an rhTNFR:Fc solution of etanercept (5 µg/g) for 4 weeks	622:677	Proteoglycan-induced mice were treated with an rhTNFR:Fc solution of etanercept (5 µg/g) for 4 weeks.
31556231	6	26	theme	proteoglycan-induced	768:787	arg1	mice					789:792	the proteoglycan-induced mice	764:792	the proteoglycan-induced mice	764:792	rhTNFR:Fc treatment attenuated the arthritis incidence and severity of arthritis in the proteoglycan-induced mice and decreased inflammation in the ankle joints and ameliorated ileal tissue destruction.
31556231	9	27	theme	serum	1100:1104	arg1	TNF-α					1106:1110	The serum TNF-α and IL-17 levels	1096:1127	TNF-α	1106:1110	The serum TNF-α and IL-17 levels were also decreased.
31556231	11	28	theme	anti-TNF-α	1357:1366	arg1	therapy					1368:1374	anti-TNF-α therapy	1357:1374	anti-TNF-α therapy	1357:1374	In conclusion, anti-TNF-α therapy attenuated proteoglycan-induced arthritis progression and modulated the gut microbiota and intestinal barrier function.
31556231	0	29	theme	Anti-TNF-α	0:9	arg1	therapy					11:17	Anti-TNF-α therapy	0:17	Anti-TNF-α therapy	0:17	Anti-TNF-α therapy alters the gut microbiota in proteoglycan-induced ankylosing spondylitis in mice.
31556231	4	30	theme	proteoglycan-induced	529:548	arg1	arthritis					550:558	proteoglycan-induced arthritis	529:558	proteoglycan-induced arthritis	529:558	Herein, the effect of a TNF-α blocker on the gut microbiota in proteoglycan-induced arthritis was investigated.
31556231	8	31	theme	rhTNFR	1085:1090	arg1	Fc					1092:1093	rhTNFR:Fc	1085:1093	rhTNFR:Fc	1085:1093	These levels were significantly restored by the administration of rhTNFR:Fc.
31556231	5	32	theme	Proteoglycan-induced	578:597	arg1	mice					599:602	Proteoglycan-induced mice	578:602	Proteoglycan-induced mice	578:602	Proteoglycan-induced mice were treated with an rhTNFR:Fc solution of etanercept (5 µg/g) for 4 weeks.
31556231	1	33	theme	Ankylosing	101:110	arg1	spondylitis					112:122	Ankylosing spondylitis	101:122	Ankylosing spondylitis	101:122	Ankylosing spondylitis is a chronic, progressive disease, and its treatment is relevant to the gut microbiota.
31556231	1	33	theme	Ankylosing	101:110	arg1	disease					150:156	a chronic, progressive disease	127:156	a chronic, progressive disease	127:156	Ankylosing spondylitis is a chronic, progressive disease, and its treatment is relevant to the gut microbiota.
31556231	6	34	from	inflammation	808:819	arg1	joints					834:839	the ankle joints	824:839	the ankle joints	824:839	rhTNFR:Fc treatment attenuated the arthritis incidence and severity of arthritis in the proteoglycan-induced mice and decreased inflammation in the ankle joints and ameliorated ileal tissue destruction.
31556231	6	35	from	destruction	870:880	arg1	joints					834:839	the ankle joints	824:839	the ankle joints	824:839	rhTNFR:Fc treatment attenuated the arthritis incidence and severity of arthritis in the proteoglycan-induced mice and decreased inflammation in the ankle joints and ameliorated ileal tissue destruction.
31556231	7	36	theme	zonula	929:934	arg1	occludens-1					936:946	zonula occludens-1	929:946	zonula occludens-1	929:946	Moreover, high gut permeability occurred, and zonula occludens-1 and occludin protein levels were reduced in proteoglycan-induced mice.
31556231	10	37	theme	Fc	1239:1240	arg1	treatment					1242:1250	rhTNFR:Fc treatment	1232:1250	rhTNFR:Fc treatment	1232:1250	In addition, flora analysis via 16S rDNA high-throughput sequencing revealed that rhTNFR:Fc treatment restored the gut microbiota composition to a composition similar to that in control mice.
31556231	8	38	theme	Fc	1092:1093	arg1	administration					1067:1080	the administration	1063:1080	the administration of rhTNFR:Fc	1063:1093	These levels were significantly restored by the administration of rhTNFR:Fc.
31556231	12	39	theme	therapy	1546:1552	arg1	strategies					1554:1563	anti-TNF-α therapy strategies	1535:1563	anti-TNF-α therapy strategies	1535:1563	These results provide new insights for anti-TNF-α therapy strategies via regulating the gut microbiota in ankylosing spondylitis.
31556231	2	40	theme	many	295:298	arg1	disease					339:345	inflammatory bowel disease	320:345	inflammatory bowel disease	320:345	Anti-tumor necrosis factor-alpha (anti-TNF-α) therapy alters the gut microbiota in many diseases, including inflammatory bowel disease.
31556231	2	40	theme	many	295:298	arg1	diseases					300:307	many diseases	295:307	many diseases	295:307	Anti-tumor necrosis factor-alpha (anti-TNF-α) therapy alters the gut microbiota in many diseases, including inflammatory bowel disease.
31556231	9	41	theme	IL-17	1116:1120	arg1	levels					1122:1127	The serum TNF-α and IL-17 levels	1096:1127	levels	1122:1127	The serum TNF-α and IL-17 levels were also decreased.
31556231	1	42	theme	gut	196:198	arg1	microbiota					200:209	the gut microbiota	192:209	the gut microbiota	192:209	Ankylosing spondylitis is a chronic, progressive disease, and its treatment is relevant to the gut microbiota.
31556231	3	43	theme	blocker	399:405	arg1	treatment					407:415	TNF-α blocker treatment	393:415	TNF-α blocker treatment	393:415	However, little is known about the effect of TNF-α blocker treatment on the gut microbiota in ankylosing spondylitis.
31556231	11	44	theme	barrier	1478:1484	arg1	function					1486:1493	the gut microbiota and intestinal barrier function	1444:1493	function	1486:1493	In conclusion, anti-TNF-α therapy attenuated proteoglycan-induced arthritis progression and modulated the gut microbiota and intestinal barrier function.
31556231	6	45	theme	tissue	863:868	arg1	destruction					870:880	ameliorated ileal tissue destruction	845:880	ameliorated ileal tissue destruction	845:880	rhTNFR:Fc treatment attenuated the arthritis incidence and severity of arthritis in the proteoglycan-induced mice and decreased inflammation in the ankle joints and ameliorated ileal tissue destruction.
31556231	10	46	theme	rhTNFR	1232:1237	arg1	treatment					1242:1250	rhTNFR:Fc treatment	1232:1250	rhTNFR:Fc treatment	1232:1250	In addition, flora analysis via 16S rDNA high-throughput sequencing revealed that rhTNFR:Fc treatment restored the gut microbiota composition to a composition similar to that in control mice.
31556231	0	47	theme	gut	30:32	arg1	microbiota					34:43	the gut microbiota	26:43	the gut microbiota in proteoglycan-induced ankylosing spondylitis in mice	26:98	Anti-TNF-α therapy alters the gut microbiota in proteoglycan-induced ankylosing spondylitis in mice.
31556231	10	48	theme	flora	1163:1167	arg1	analysis					1169:1176	flora analysis	1163:1176	flora analysis via 16S rDNA high-throughput sequencing	1163:1216	In addition, flora analysis via 16S rDNA high-throughput sequencing revealed that rhTNFR:Fc treatment restored the gut microbiota composition to a composition similar to that in control mice.
31556231	3	49	theme	treatment	407:415	arg1	effect					383:388	the effect	379:388	the effect of TNF-α blocker treatment on the gut microbiota in ankylosing spondylitis	379:463	However, little is known about the effect of TNF-α blocker treatment on the gut microbiota in ankylosing spondylitis.
31556231	0	50	from	spondylitis	80:90	arg1	mice					95:98	mice	95:98	mice	95:98	Anti-TNF-α therapy alters the gut microbiota in proteoglycan-induced ankylosing spondylitis in mice.
31556231	2	51	theme	factor-alpha	232:243	arg1	therapy					258:264	Anti-tumor necrosis factor-alpha (anti-TNF-α) therapy	212:264	Anti-tumor necrosis factor-alpha (anti-TNF-α) therapy	212:264	Anti-tumor necrosis factor-alpha (anti-TNF-α) therapy alters the gut microbiota in many diseases, including inflammatory bowel disease.
31556231	6	52	theme	arthritis	751:759	arg1	severity					739:746	severity	739:746	severity	739:746	rhTNFR:Fc treatment attenuated the arthritis incidence and severity of arthritis in the proteoglycan-induced mice and decreased inflammation in the ankle joints and ameliorated ileal tissue destruction.
31556231	6	52	theme	arthritis	751:759	arg1	incidence					725:733	arthritis incidence	715:733	arthritis incidence	715:733	rhTNFR:Fc treatment attenuated the arthritis incidence and severity of arthritis in the proteoglycan-induced mice and decreased inflammation in the ankle joints and ameliorated ileal tissue destruction.
31556231	3	53	theme	ankylosing	442:451	arg1	spondylitis					453:463	ankylosing spondylitis	442:463	ankylosing spondylitis	442:463	However, little is known about the effect of TNF-α blocker treatment on the gut microbiota in ankylosing spondylitis.
31556231	0	54	theme	proteoglycan-induced	48:67	arg1	spondylitis					80:90	proteoglycan-induced ankylosing spondylitis	48:90	proteoglycan-induced ankylosing spondylitis in mice	48:98	Anti-TNF-α therapy alters the gut microbiota in proteoglycan-induced ankylosing spondylitis in mice.
31556231	10	55	theme	similar	1309:1315	arg1	composition					1297:1307	a composition	1295:1307	a composition similar to that in control mice	1295:1339	In addition, flora analysis via 16S rDNA high-throughput sequencing revealed that rhTNFR:Fc treatment restored the gut microbiota composition to a composition similar to that in control mice.
31556231	6	56	theme	ameliorated	845:855	arg1	destruction					870:880	ameliorated ileal tissue destruction	845:880	ameliorated ileal tissue destruction	845:880	rhTNFR:Fc treatment attenuated the arthritis incidence and severity of arthritis in the proteoglycan-induced mice and decreased inflammation in the ankle joints and ameliorated ileal tissue destruction.
31556231	2	57	theme	necrosis	223:230	arg1	factor-alpha					232:243	Anti-tumor necrosis factor-alpha	212:243	Anti-tumor necrosis factor-alpha (anti-TNF-α) therapy	212:264	Anti-tumor necrosis factor-alpha (anti-TNF-α) therapy alters the gut microbiota in many diseases, including inflammatory bowel disease.
31556231	2	57	theme	necrosis	223:230	arg1	anti-TNF-α					246:255	anti-TNF-α	246:255	anti-TNF-α	246:255	Anti-tumor necrosis factor-alpha (anti-TNF-α) therapy alters the gut microbiota in many diseases, including inflammatory bowel disease.
31556231	12	58	from	microbiota	1588:1597	arg1	spondylitis					1613:1623	ankylosing spondylitis	1602:1623	ankylosing spondylitis	1602:1623	These results provide new insights for anti-TNF-α therapy strategies via regulating the gut microbiota in ankylosing spondylitis.
31556231	6	59	from	severity	739:746	arg1	mice					789:792	the proteoglycan-induced mice	764:792	the proteoglycan-induced mice	764:792	rhTNFR:Fc treatment attenuated the arthritis incidence and severity of arthritis in the proteoglycan-induced mice and decreased inflammation in the ankle joints and ameliorated ileal tissue destruction.
31556231	3	60	theme	TNF-α	393:397	arg1	treatment					407:415	TNF-α blocker treatment	393:415	TNF-α blocker treatment	393:415	However, little is known about the effect of TNF-α blocker treatment on the gut microbiota in ankylosing spondylitis.
31556231	12	61	theme	new	1518:1520	arg1	insights					1522:1529	new insights	1518:1529	new insights for anti-TNF-α therapy strategies	1518:1563	These results provide new insights for anti-TNF-α therapy strategies via regulating the gut microbiota in ankylosing spondylitis.
31556231	2	62	theme	Anti-tumor	212:221	arg1	factor-alpha					232:243	Anti-tumor necrosis factor-alpha	212:243	Anti-tumor necrosis factor-alpha (anti-TNF-α) therapy	212:264	Anti-tumor necrosis factor-alpha (anti-TNF-α) therapy alters the gut microbiota in many diseases, including inflammatory bowel disease.
31556231	2	62	theme	Anti-tumor	212:221	arg1	anti-TNF-α					246:255	anti-TNF-α	246:255	anti-TNF-α	246:255	Anti-tumor necrosis factor-alpha (anti-TNF-α) therapy alters the gut microbiota in many diseases, including inflammatory bowel disease.
31556231	1	63	theme	chronic	129:135	arg1	spondylitis					112:122	Ankylosing spondylitis	101:122	Ankylosing spondylitis	101:122	Ankylosing spondylitis is a chronic, progressive disease, and its treatment is relevant to the gut microbiota.
31556231	1	63	theme	chronic	129:135	arg1	disease					150:156	a chronic, progressive disease	127:156	a chronic, progressive disease	127:156	Ankylosing spondylitis is a chronic, progressive disease, and its treatment is relevant to the gut microbiota.
31556231	12	64	theme	anti-TNF-α	1535:1544	arg1	strategies					1554:1563	anti-TNF-α therapy strategies	1535:1563	anti-TNF-α therapy strategies	1535:1563	These results provide new insights for anti-TNF-α therapy strategies via regulating the gut microbiota in ankylosing spondylitis.
31556231	4	65	theme	gut	511:513	arg1	microbiota					515:524	the gut microbiota	507:524	the gut microbiota in proteoglycan-induced arthritis	507:558	Herein, the effect of a TNF-α blocker on the gut microbiota in proteoglycan-induced arthritis was investigated.
31556231	10	66	theme	control	1328:1334	arg1	mice					1336:1339	control mice	1328:1339	control mice	1328:1339	In addition, flora analysis via 16S rDNA high-throughput sequencing revealed that rhTNFR:Fc treatment restored the gut microbiota composition to a composition similar to that in control mice.
31556231	2	67	theme	bowel	333:337	arg1	disease					339:345	inflammatory bowel disease	320:345	inflammatory bowel disease	320:345	Anti-tumor necrosis factor-alpha (anti-TNF-α) therapy alters the gut microbiota in many diseases, including inflammatory bowel disease.
31556231	10	68	theme	microbiota	1269:1278	arg1	composition					1280:1290	the gut microbiota composition	1261:1290	the gut microbiota composition	1261:1290	In addition, flora analysis via 16S rDNA high-throughput sequencing revealed that rhTNFR:Fc treatment restored the gut microbiota composition to a composition similar to that in control mice.
31556231	10	69	theme	rDNA	1186:1189	arg1	sequencing					1207:1216	16S rDNA high-throughput sequencing	1182:1216	16S rDNA high-throughput sequencing	1182:1216	In addition, flora analysis via 16S rDNA high-throughput sequencing revealed that rhTNFR:Fc treatment restored the gut microbiota composition to a composition similar to that in control mice.
31556231	6	70	theme	ankle	828:832	arg1	joints					834:839	the ankle joints	824:839	the ankle joints	824:839	rhTNFR:Fc treatment attenuated the arthritis incidence and severity of arthritis in the proteoglycan-induced mice and decreased inflammation in the ankle joints and ameliorated ileal tissue destruction.
31556231	7	71	theme	proteoglycan-induced	992:1011	arg1	mice					1013:1016	proteoglycan-induced mice	992:1016	proteoglycan-induced mice	992:1016	Moreover, high gut permeability occurred, and zonula occludens-1 and occludin protein levels were reduced in proteoglycan-induced mice.
31556231	2	72	theme	inflammatory	320:331	arg1	disease					339:345	inflammatory bowel disease	320:345	inflammatory bowel disease	320:345	Anti-tumor necrosis factor-alpha (anti-TNF-α) therapy alters the gut microbiota in many diseases, including inflammatory bowel disease.
31556231	6	73	dep	incidence	725:733	arg1	the					711:713	the	711:713	the	711:713	rhTNFR:Fc treatment attenuated the arthritis incidence and severity of arthritis in the proteoglycan-induced mice and decreased inflammation in the ankle joints and ameliorated ileal tissue destruction.
31556231	10	74	theme	16S	1182:1184	arg1	sequencing					1207:1216	16S rDNA high-throughput sequencing	1182:1216	16S rDNA high-throughput sequencing	1182:1216	In addition, flora analysis via 16S rDNA high-throughput sequencing revealed that rhTNFR:Fc treatment restored the gut microbiota composition to a composition similar to that in control mice.
31556231	11	75	theme	arthritis	1408:1416	arg1	progression					1418:1428	proteoglycan-induced arthritis progression	1387:1428	proteoglycan-induced arthritis progression	1387:1428	In conclusion, anti-TNF-α therapy attenuated proteoglycan-induced arthritis progression and modulated the gut microbiota and intestinal barrier function.
31556231	6	76	theme	arthritis	715:723	arg1	incidence					725:733	arthritis incidence	715:733	arthritis incidence	715:733	rhTNFR:Fc treatment attenuated the arthritis incidence and severity of arthritis in the proteoglycan-induced mice and decreased inflammation in the ankle joints and ameliorated ileal tissue destruction.
31556231	2	77	from	microbiota	281:290	arg1	disease					339:345	inflammatory bowel disease	320:345	inflammatory bowel disease	320:345	Anti-tumor necrosis factor-alpha (anti-TNF-α) therapy alters the gut microbiota in many diseases, including inflammatory bowel disease.
31556231	2	77	from	microbiota	281:290	arg1	diseases					300:307	many diseases	295:307	many diseases	295:307	Anti-tumor necrosis factor-alpha (anti-TNF-α) therapy alters the gut microbiota in many diseases, including inflammatory bowel disease.
31556231	0	78	theme	ankylosing	69:78	arg1	spondylitis					80:90	proteoglycan-induced ankylosing spondylitis	48:90	proteoglycan-induced ankylosing spondylitis in mice	48:98	Anti-TNF-α therapy alters the gut microbiota in proteoglycan-induced ankylosing spondylitis in mice.
31556231	12	79	theme	ankylosing	1602:1611	arg1	spondylitis					1613:1623	ankylosing spondylitis	1602:1623	ankylosing spondylitis	1602:1623	These results provide new insights for anti-TNF-α therapy strategies via regulating the gut microbiota in ankylosing spondylitis.
31556231	10	80	theme	gut	1265:1267	arg1	composition					1280:1290	the gut microbiota composition	1261:1290	the gut microbiota composition	1261:1290	In addition, flora analysis via 16S rDNA high-throughput sequencing revealed that rhTNFR:Fc treatment restored the gut microbiota composition to a composition similar to that in control mice.
31313786	0	0	theme	cellular	93:100	arg1	boundaries					102:111	crowded cellular boundaries	85:111	crowded cellular boundaries	85:111	Engineering of spectator glycocalyx structures to evaluate molecular interactions at crowded cellular boundaries.
31313786	4	1	from	ligands	880:886	arg1	surface					895:901	the surface	891:901	the surface of the remodelled cells	891:925	We evaluated the association of two lectins, ConA and SNA, with their endogenous glycan ligands on the surface of the remodelled cells.
31313786	4	2	theme	glycan	873:878	arg1	ligands					880:886	their endogenous glycan ligands	856:886	their endogenous glycan ligands on the surface of the remodelled cells	856:925	We evaluated the association of two lectins, ConA and SNA, with their endogenous glycan ligands on the surface of the remodelled cells.
31313786	3	3	theme	mucin	650:654	arg1	glycoproteins					656:668	mucin glycoproteins	650:668	mucin glycoproteins	650:668	Here, we report the synthesis of PEG-based glycopolymers with tunable glycan composition, which approximate the extended architecture of mucin glycoproteins, and tether them to the plasma membranes of red blood cells (RBC) to construct an artificial mucin brush-like glycocalyx.
31313786	6	4	theme	RBC	1372:1374	arg1	glycocalyx					1376:1385	the native RBC glycocalyx	1361:1385	the native RBC glycocalyx	1361:1385	The different sensitivity of ConA and SNA to glycocalyx crowding likely arises from the distinct presentation of their mannoside and sialoside receptors, respectively, within the native RBC glycocalyx.
31313786	1	5	theme	physical	249:256	arg1	barrier					258:264	a physical barrier	247:264	a physical barrier that provides protective functions, mediates the exchange of nutrients and regulates cellular interactions	247:371	In the mucosal epithelium, the cellular glycocalyx can project tens to hundreds of nanometers into the extracellular space, erecting a physical barrier that provides protective functions, mediates the exchange of nutrients and regulates cellular interactions.
31313786	0	6	theme	crowded	85:91	arg1	boundaries					102:111	crowded cellular boundaries	85:111	crowded cellular boundaries	85:111	Engineering of spectator glycocalyx structures to evaluate molecular interactions at crowded cellular boundaries.
31313786	1	7	theme	cellular	351:358	arg1	interactions					360:371	cellular interactions	351:371	cellular interactions	351:371	In the mucosal epithelium, the cellular glycocalyx can project tens to hundreds of nanometers into the extracellular space, erecting a physical barrier that provides protective functions, mediates the exchange of nutrients and regulates cellular interactions.
31313786	4	8	theme	endogenous	862:871	arg1	ligands					880:886	their endogenous glycan ligands	856:886	their endogenous glycan ligands on the surface of the remodelled cells	856:925	We evaluated the association of two lectins, ConA and SNA, with their endogenous glycan ligands on the surface of the remodelled cells.
31313786	5	9	theme	ConA	1054:1057	arg1	binding					1059:1065	ConA binding	1054:1065	ConA binding	1054:1065	The extended glycocalyx provided protection against agglutination of RBCs by both lectins; however, the rate and magnitude of ConA binding were attenuated to a greater degree in the presence of the glycopolymer spectators compared to those measured for SNA.
31313786	6	10	theme	distinct	1274:1281	arg1	presentation					1283:1294	the distinct presentation	1270:1294	the distinct presentation of their mannoside and sialoside receptors, respectively	1270:1351	The different sensitivity of ConA and SNA to glycocalyx crowding likely arises from the distinct presentation of their mannoside and sialoside receptors, respectively, within the native RBC glycocalyx.
31313786	3	11	theme	blood	718:722	arg1	cells					724:728	red blood cells	714:728	red blood cells (RBC)	714:734	Here, we report the synthesis of PEG-based glycopolymers with tunable glycan composition, which approximate the extended architecture of mucin glycoproteins, and tether them to the plasma membranes of red blood cells (RBC) to construct an artificial mucin brush-like glycocalyx.
31313786	3	11	theme	blood	718:722	arg1	RBC					731:733	RBC	731:733	RBC	731:733	Here, we report the synthesis of PEG-based glycopolymers with tunable glycan composition, which approximate the extended architecture of mucin glycoproteins, and tether them to the plasma membranes of red blood cells (RBC) to construct an artificial mucin brush-like glycocalyx.
31313786	1	12	from	project	169:175	arg1	epithelium					129:138	the mucosal epithelium	117:138	the mucosal epithelium	117:138	In the mucosal epithelium, the cellular glycocalyx can project tens to hundreds of nanometers into the extracellular space, erecting a physical barrier that provides protective functions, mediates the exchange of nutrients and regulates cellular interactions.
31313786	4	13	with	association	809:819	arg1	ligands					880:886	their endogenous glycan ligands	856:886	their endogenous glycan ligands on the surface of the remodelled cells	856:925	We evaluated the association of two lectins, ConA and SNA, with their endogenous glycan ligands on the surface of the remodelled cells.
31313786	3	14	theme	red	714:716	arg1	cells					724:728	red blood cells	714:728	red blood cells (RBC)	714:734	Here, we report the synthesis of PEG-based glycopolymers with tunable glycan composition, which approximate the extended architecture of mucin glycoproteins, and tether them to the plasma membranes of red blood cells (RBC) to construct an artificial mucin brush-like glycocalyx.
31313786	3	14	theme	red	714:716	arg1	RBC					731:733	RBC	731:733	RBC	731:733	Here, we report the synthesis of PEG-based glycopolymers with tunable glycan composition, which approximate the extended architecture of mucin glycoproteins, and tether them to the plasma membranes of red blood cells (RBC) to construct an artificial mucin brush-like glycocalyx.
31313786	3	15	theme	tunable	575:581	arg1	composition					590:600	tunable glycan composition	575:600	tunable glycan composition	575:600	Here, we report the synthesis of PEG-based glycopolymers with tunable glycan composition, which approximate the extended architecture of mucin glycoproteins, and tether them to the plasma membranes of red blood cells (RBC) to construct an artificial mucin brush-like glycocalyx.
31313786	5	16	theme	greater	1088:1094	arg1	degree					1096:1101	a greater degree	1086:1101	a greater degree	1086:1101	The extended glycocalyx provided protection against agglutination of RBCs by both lectins; however, the rate and magnitude of ConA binding were attenuated to a greater degree in the presence of the glycopolymer spectators compared to those measured for SNA.
31313786	3	17	theme	PEG-based	546:554	arg1	glycopolymers					556:568	PEG-based glycopolymers	546:568	PEG-based glycopolymers with tunable glycan composition, which approximate the extended architecture of mucin glycoproteins	546:668	Here, we report the synthesis of PEG-based glycopolymers with tunable glycan composition, which approximate the extended architecture of mucin glycoproteins, and tether them to the plasma membranes of red blood cells (RBC) to construct an artificial mucin brush-like glycocalyx.
31313786	2	18	theme	physical	409:416	arg1	properties					418:427	the physical properties	405:427	the physical properties of the mucosal glycocalyx	405:453	Little is understood about how the physical properties of the mucosal glycocalyx influence molecular recognition at the cellular boundary.
31313786	3	19	with	glycopolymers	556:568	arg1	composition					590:600	tunable glycan composition	575:600	tunable glycan composition	575:600	Here, we report the synthesis of PEG-based glycopolymers with tunable glycan composition, which approximate the extended architecture of mucin glycoproteins, and tether them to the plasma membranes of red blood cells (RBC) to construct an artificial mucin brush-like glycocalyx.
31313786	3	20	theme	glycopolymers	556:568	arg1	synthesis					533:541	the synthesis	529:541	the synthesis of PEG-based glycopolymers with tunable glycan composition, which approximate the extended architecture of mucin glycoproteins	529:668	Here, we report the synthesis of PEG-based glycopolymers with tunable glycan composition, which approximate the extended architecture of mucin glycoproteins, and tether them to the plasma membranes of red blood cells (RBC) to construct an artificial mucin brush-like glycocalyx.
31313786	3	21	gly	glycoproteins	656:668	arg1	glycoproteins					656:668	mucin glycoproteins	650:668	mucin glycoproteins	650:668	Here, we report the synthesis of PEG-based glycopolymers with tunable glycan composition, which approximate the extended architecture of mucin glycoproteins, and tether them to the plasma membranes of red blood cells (RBC) to construct an artificial mucin brush-like glycocalyx.
31313786	6	22	theme	native	1365:1370	arg1	glycocalyx					1376:1385	the native RBC glycocalyx	1361:1385	the native RBC glycocalyx	1361:1385	The different sensitivity of ConA and SNA to glycocalyx crowding likely arises from the distinct presentation of their mannoside and sialoside receptors, respectively, within the native RBC glycocalyx.
31313786	6	23	theme	glycocalyx	1231:1240	arg1	crowding					1242:1249	glycocalyx crowding	1231:1249	glycocalyx crowding	1231:1249	The different sensitivity of ConA and SNA to glycocalyx crowding likely arises from the distinct presentation of their mannoside and sialoside receptors, respectively, within the native RBC glycocalyx.
31313786	3	24	theme	mucin	763:767	arg1	glycocalyx					780:789	an artificial mucin brush-like glycocalyx	749:789	an artificial mucin brush-like glycocalyx	749:789	Here, we report the synthesis of PEG-based glycopolymers with tunable glycan composition, which approximate the extended architecture of mucin glycoproteins, and tether them to the plasma membranes of red blood cells (RBC) to construct an artificial mucin brush-like glycocalyx.
31313786	5	25	theme	extended	932:939	arg1	glycocalyx					941:950	The extended glycocalyx	928:950	The extended glycocalyx	928:950	The extended glycocalyx provided protection against agglutination of RBCs by both lectins; however, the rate and magnitude of ConA binding were attenuated to a greater degree in the presence of the glycopolymer spectators compared to those measured for SNA.
31313786	2	26	theme	molecular	465:473	arg1	recognition					475:485	molecular recognition	465:485	molecular recognition	465:485	Little is understood about how the physical properties of the mucosal glycocalyx influence molecular recognition at the cellular boundary.
31313786	1	27	theme	protective	280:289	arg1	functions					291:299	protective functions	280:299	protective functions	280:299	In the mucosal epithelium, the cellular glycocalyx can project tens to hundreds of nanometers into the extracellular space, erecting a physical barrier that provides protective functions, mediates the exchange of nutrients and regulates cellular interactions.
31313786	3	28	theme	plasma	694:699	arg1	membranes					701:709	the plasma membranes	690:709	the plasma membranes of red blood cells (RBC)	690:734	Here, we report the synthesis of PEG-based glycopolymers with tunable glycan composition, which approximate the extended architecture of mucin glycoproteins, and tether them to the plasma membranes of red blood cells (RBC) to construct an artificial mucin brush-like glycocalyx.
31313786	6	29	theme	receptors	1329:1337	arg1	presentation					1283:1294	the distinct presentation	1270:1294	the distinct presentation of their mannoside and sialoside receptors, respectively	1270:1351	The different sensitivity of ConA and SNA to glycocalyx crowding likely arises from the distinct presentation of their mannoside and sialoside receptors, respectively, within the native RBC glycocalyx.
31313786	0	30	theme	glycocalyx	25:34	arg1	structures					36:45	spectator glycocalyx structures	15:45	spectator glycocalyx structures	15:45	Engineering of spectator glycocalyx structures to evaluate molecular interactions at crowded cellular boundaries.
31313786	3	31	theme	brush-like	769:778	arg1	glycocalyx					780:789	an artificial mucin brush-like glycocalyx	749:789	an artificial mucin brush-like glycocalyx	749:789	Here, we report the synthesis of PEG-based glycopolymers with tunable glycan composition, which approximate the extended architecture of mucin glycoproteins, and tether them to the plasma membranes of red blood cells (RBC) to construct an artificial mucin brush-like glycocalyx.
31313786	4	32	theme	cells	921:925	arg1	surface					895:901	the surface	891:901	the surface of the remodelled cells	891:925	We evaluated the association of two lectins, ConA and SNA, with their endogenous glycan ligands on the surface of the remodelled cells.
31313786	3	33	theme	glycan	583:588	arg1	composition					590:600	tunable glycan composition	575:600	tunable glycan composition	575:600	Here, we report the synthesis of PEG-based glycopolymers with tunable glycan composition, which approximate the extended architecture of mucin glycoproteins, and tether them to the plasma membranes of red blood cells (RBC) to construct an artificial mucin brush-like glycocalyx.
31313786	6	34	theme	SNA	1224:1226	arg1	sensitivity					1200:1210	The different sensitivity	1186:1210	The different sensitivity of ConA and SNA to glycocalyx crowding	1186:1249	The different sensitivity of ConA and SNA to glycocalyx crowding likely arises from the distinct presentation of their mannoside and sialoside receptors, respectively, within the native RBC glycocalyx.
31313786	2	35	theme	mucosal	436:442	arg1	glycocalyx					444:453	the mucosal glycocalyx	432:453	the mucosal glycocalyx	432:453	Little is understood about how the physical properties of the mucosal glycocalyx influence molecular recognition at the cellular boundary.
31313786	2	36	theme	glycocalyx	444:453	arg1	properties					418:427	the physical properties	405:427	the physical properties of the mucosal glycocalyx	405:453	Little is understood about how the physical properties of the mucosal glycocalyx influence molecular recognition at the cellular boundary.
31313786	4	37	theme	remodelled	910:919	arg1	cells					921:925	the remodelled cells	906:925	the remodelled cells	906:925	We evaluated the association of two lectins, ConA and SNA, with their endogenous glycan ligands on the surface of the remodelled cells.
31313786	5	38	theme	binding	1059:1065	arg1	magnitude					1041:1049	magnitude	1041:1049	magnitude	1041:1049	The extended glycocalyx provided protection against agglutination of RBCs by both lectins; however, the rate and magnitude of ConA binding were attenuated to a greater degree in the presence of the glycopolymer spectators compared to those measured for SNA.
31313786	5	38	theme	binding	1059:1065	arg1	rate					1032:1035	rate	1032:1035	rate	1032:1035	The extended glycocalyx provided protection against agglutination of RBCs by both lectins; however, the rate and magnitude of ConA binding were attenuated to a greater degree in the presence of the glycopolymer spectators compared to those measured for SNA.
31313786	3	39	theme	artificial	752:761	arg1	glycocalyx					780:789	an artificial mucin brush-like glycocalyx	749:789	an artificial mucin brush-like glycocalyx	749:789	Here, we report the synthesis of PEG-based glycopolymers with tunable glycan composition, which approximate the extended architecture of mucin glycoproteins, and tether them to the plasma membranes of red blood cells (RBC) to construct an artificial mucin brush-like glycocalyx.
31313786	6	40	theme	sialoside	1319:1327	arg1	receptors					1329:1337	sialoside receptors	1319:1337	sialoside receptors	1319:1337	The different sensitivity of ConA and SNA to glycocalyx crowding likely arises from the distinct presentation of their mannoside and sialoside receptors, respectively, within the native RBC glycocalyx.
31313786	3	41	theme	extended	625:632	arg1	architecture					634:645	the extended architecture	621:645	the extended architecture of mucin glycoproteins	621:668	Here, we report the synthesis of PEG-based glycopolymers with tunable glycan composition, which approximate the extended architecture of mucin glycoproteins, and tether them to the plasma membranes of red blood cells (RBC) to construct an artificial mucin brush-like glycocalyx.
31313786	0	42	theme	structures	36:45	arg1	Engineering					0:10	Engineering	0:10	Engineering of spectator glycocalyx structures	0:45	Engineering of spectator glycocalyx structures to evaluate molecular interactions at crowded cellular boundaries.
31313786	5	43	theme	glycopolymer	1126:1137	arg1	spectators					1139:1148	the glycopolymer spectators	1122:1148	the glycopolymer spectators	1122:1148	The extended glycocalyx provided protection against agglutination of RBCs by both lectins; however, the rate and magnitude of ConA binding were attenuated to a greater degree in the presence of the glycopolymer spectators compared to those measured for SNA.
31313786	2	44	theme	cellular	494:501	arg1	boundary					503:510	the cellular boundary	490:510	the cellular boundary	490:510	Little is understood about how the physical properties of the mucosal glycocalyx influence molecular recognition at the cellular boundary.
31313786	4	45	theme	lectins	828:834	arg1	association					809:819	the association	805:819	the association of two lectins, ConA and SNA, with their endogenous glycan ligands on the surface of the remodelled cells	805:925	We evaluated the association of two lectins, ConA and SNA, with their endogenous glycan ligands on the surface of the remodelled cells.
31313786	1	46	theme	extracellular	217:229	arg1	space					231:235	the extracellular space	213:235	the extracellular space	213:235	In the mucosal epithelium, the cellular glycocalyx can project tens to hundreds of nanometers into the extracellular space, erecting a physical barrier that provides protective functions, mediates the exchange of nutrients and regulates cellular interactions.
31313786	0	47	theme	molecular	59:67	arg1	interactions					69:80	molecular interactions	59:80	molecular interactions at crowded cellular boundaries	59:111	Engineering of spectator glycocalyx structures to evaluate molecular interactions at crowded cellular boundaries.
31313786	5	48	theme	spectators	1139:1148	arg1	presence					1110:1117	the presence	1106:1117	the presence of the glycopolymer spectators	1106:1148	The extended glycocalyx provided protection against agglutination of RBCs by both lectins; however, the rate and magnitude of ConA binding were attenuated to a greater degree in the presence of the glycopolymer spectators compared to those measured for SNA.
31313786	6	49	theme	mannoside	1305:1313	arg1	presentation					1283:1294	the distinct presentation	1270:1294	the distinct presentation of their mannoside and sialoside receptors, respectively	1270:1351	The different sensitivity of ConA and SNA to glycocalyx crowding likely arises from the distinct presentation of their mannoside and sialoside receptors, respectively, within the native RBC glycocalyx.
31313786	6	50	theme	ConA	1215:1218	arg1	sensitivity					1200:1210	The different sensitivity	1186:1210	The different sensitivity of ConA and SNA to glycocalyx crowding	1186:1249	The different sensitivity of ConA and SNA to glycocalyx crowding likely arises from the distinct presentation of their mannoside and sialoside receptors, respectively, within the native RBC glycocalyx.
31313786	1	51	theme	mucosal	121:127	arg1	epithelium					129:138	the mucosal epithelium	117:138	the mucosal epithelium	117:138	In the mucosal epithelium, the cellular glycocalyx can project tens to hundreds of nanometers into the extracellular space, erecting a physical barrier that provides protective functions, mediates the exchange of nutrients and regulates cellular interactions.
31313786	1	52	theme	nutrients	327:335	arg1	exchange					315:322	the exchange	311:322	the exchange of nutrients	311:335	In the mucosal epithelium, the cellular glycocalyx can project tens to hundreds of nanometers into the extracellular space, erecting a physical barrier that provides protective functions, mediates the exchange of nutrients and regulates cellular interactions.
31313786	3	53	theme	glycoproteins	656:668	arg1	architecture					634:645	the extended architecture	621:645	the extended architecture of mucin glycoproteins	621:668	Here, we report the synthesis of PEG-based glycopolymers with tunable glycan composition, which approximate the extended architecture of mucin glycoproteins, and tether them to the plasma membranes of red blood cells (RBC) to construct an artificial mucin brush-like glycocalyx.
31313786	5	54	theme	RBCs	997:1000	arg1	agglutination					980:992	agglutination	980:992	agglutination of RBCs	980:1000	The extended glycocalyx provided protection against agglutination of RBCs by both lectins; however, the rate and magnitude of ConA binding were attenuated to a greater degree in the presence of the glycopolymer spectators compared to those measured for SNA.
31313786	6	55	theme	different	1190:1198	arg1	sensitivity					1200:1210	The different sensitivity	1186:1210	The different sensitivity of ConA and SNA to glycocalyx crowding	1186:1249	The different sensitivity of ConA and SNA to glycocalyx crowding likely arises from the distinct presentation of their mannoside and sialoside receptors, respectively, within the native RBC glycocalyx.
31313786	0	56	from	boundaries	102:111	arg1	interactions					69:80	molecular interactions	59:80	molecular interactions at crowded cellular boundaries	59:111	Engineering of spectator glycocalyx structures to evaluate molecular interactions at crowded cellular boundaries.
31313786	1	57	theme	nanometers	197:206	arg1	hundreds					185:192	hundreds	185:192	hundreds of nanometers	185:206	In the mucosal epithelium, the cellular glycocalyx can project tens to hundreds of nanometers into the extracellular space, erecting a physical barrier that provides protective functions, mediates the exchange of nutrients and regulates cellular interactions.
31313786	1	58	theme	cellular	145:152	arg1	glycocalyx					154:163	the cellular glycocalyx	141:163	the cellular glycocalyx	141:163	In the mucosal epithelium, the cellular glycocalyx can project tens to hundreds of nanometers into the extracellular space, erecting a physical barrier that provides protective functions, mediates the exchange of nutrients and regulates cellular interactions.
31313786	5	59	dep	rate	1032:1035	arg1	the					1028:1030	the	1028:1030	the	1028:1030	The extended glycocalyx provided protection against agglutination of RBCs by both lectins; however, the rate and magnitude of ConA binding were attenuated to a greater degree in the presence of the glycopolymer spectators compared to those measured for SNA.
31313786	3	60	theme	cells	724:728	arg1	membranes					701:709	the plasma membranes	690:709	the plasma membranes of red blood cells (RBC)	690:734	Here, we report the synthesis of PEG-based glycopolymers with tunable glycan composition, which approximate the extended architecture of mucin glycoproteins, and tether them to the plasma membranes of red blood cells (RBC) to construct an artificial mucin brush-like glycocalyx.
31313786	0	61	theme	spectator	15:23	arg1	structures					36:45	spectator glycocalyx structures	15:45	spectator glycocalyx structures	15:45	Engineering of spectator glycocalyx structures to evaluate molecular interactions at crowded cellular boundaries.
31293526	11	0	from	GroL	1639:1642	arg1	matrix					1584:1589	the matrix	1580:1589	the matrix	1580:1589	We detected 447 proteins in the matrix, of which the most abundant were the chaperonin GroL, the elongation factors EF-Tu and EF-G, several enzymes of glycolysis, and proteins of unknown function.
31293526	4	1	theme	biofilm	564:570	arg1	matrix					572:577	the biofilm matrix	560:577	the biofilm matrix of C. acnes	560:589	However, little is known about the actual composition of the biofilm matrix of C. acnes.
31293526	10	2	theme	resonance	1418:1426	arg1	technique					1428:1436	a nuclear magnetic resonance technique	1399:1436	a nuclear magnetic resonance technique	1399:1436	The chemical structure of the major polysaccharide was determined using a nuclear magnetic resonance technique, the formula being →6)-α-D-Galp-(1→4)-β-D-ManpNAc3NAcA-(1→6)-α-D-Glcp-(1→4)-β-D-ManpNAc3NAcA-(1→3)-β-GalpNAc-(1→.
31293526	10	2	theme	resonance	1418:1426	arg1	formula					1443:1449	the formula	1439:1449	the formula being →6)-α-D-Galp-(1→4)-β-D-ManpNAc3NAcA-(1→6)-α-D-Glcp-(1→4)-β-D-ManpNAc3NAcA-(1→3)-β-GalpNAc-(1→	1439:1549	The chemical structure of the major polysaccharide was determined using a nuclear magnetic resonance technique, the formula being →6)-α-D-Galp-(1→4)-β-D-ManpNAc3NAcA-(1→6)-α-D-Glcp-(1→4)-β-D-ManpNAc3NAcA-(1→3)-β-GalpNAc-(1→.
31293526	14	3	theme	matrix	2199:2204	arg1	extraction					2206:2215	successful matrix extraction	2188:2215	successful matrix extraction rather than simply the presence of cell debris	2188:2262	The difference between the matrix and biofilm biomass spectra showed successful matrix extraction rather than simply the presence of cell debris after sonication.
31293526	11	4	theme	several	1684:1690	arg1	enzymes					1692:1698	several enzymes	1684:1698	several enzymes of glycolysis	1684:1712	We detected 447 proteins in the matrix, of which the most abundant were the chaperonin GroL, the elongation factors EF-Tu and EF-G, several enzymes of glycolysis, and proteins of unknown function.
31293526	13	5	theme	RT5	1968:1970	arg1	matrix					1972:1977	the C. acnes RT5 matrix	1955:1977	the C. acnes RT5 matrix	1955:1977	We also performed surface-enhanced Raman spectroscopy analysis of the C. acnes RT5 matrix for the first time, providing the surface-enhanced Raman scattering (SERS) profiles of the C. acnes RT5 biofilm matrix and biofilm biomass.
31293526	8	6	theme	C.	1094:1095	arg1	strain					1107:1112	the acneic C. acnes RT5 strain	1083:1112	the acneic C. acnes RT5 strain	1083:1112	The biofilm matrix of the acneic C. acnes RT5 strain appears to consist mainly of polysaccharides.
31293526	8	7	theme	RT5	1103:1105	arg1	strain					1107:1112	the acneic C. acnes RT5 strain	1083:1112	the acneic C. acnes RT5 strain	1083:1112	The biofilm matrix of the acneic C. acnes RT5 strain appears to consist mainly of polysaccharides.
31293526	11	8	theme	glycolysis	1703:1712	arg1	factors					1660:1666	the elongation factors	1645:1666	the elongation factors EF-Tu and EF-G	1645:1681	We detected 447 proteins in the matrix, of which the most abundant were the chaperonin GroL, the elongation factors EF-Tu and EF-G, several enzymes of glycolysis, and proteins of unknown function.
31293526	11	8	theme	glycolysis	1703:1712	arg1	EF-Tu					1668:1672	EF-Tu	1668:1672	EF-Tu	1668:1672	We detected 447 proteins in the matrix, of which the most abundant were the chaperonin GroL, the elongation factors EF-Tu and EF-G, several enzymes of glycolysis, and proteins of unknown function.
31293526	11	8	theme	glycolysis	1703:1712	arg1	proteins					1568:1575	447 proteins	1564:1575	447 proteins	1564:1575	We detected 447 proteins in the matrix, of which the most abundant were the chaperonin GroL, the elongation factors EF-Tu and EF-G, several enzymes of glycolysis, and proteins of unknown function.
31293526	11	8	theme	glycolysis	1703:1712	arg1	EF-G					1678:1681	EF-G	1678:1681	EF-G	1678:1681	We detected 447 proteins in the matrix, of which the most abundant were the chaperonin GroL, the elongation factors EF-Tu and EF-G, several enzymes of glycolysis, and proteins of unknown function.
31293526	11	8	theme	glycolysis	1703:1712	arg1	proteins					1719:1726	proteins	1719:1726	proteins of unknown function	1719:1746	We detected 447 proteins in the matrix, of which the most abundant were the chaperonin GroL, the elongation factors EF-Tu and EF-G, several enzymes of glycolysis, and proteins of unknown function.
31293526	11	8	theme	glycolysis	1703:1712	arg1	enzymes					1692:1698	several enzymes	1684:1698	several enzymes of glycolysis	1684:1712	We detected 447 proteins in the matrix, of which the most abundant were the chaperonin GroL, the elongation factors EF-Tu and EF-G, several enzymes of glycolysis, and proteins of unknown function.
31293526	14	9	theme	biomass	2165:2171	arg1	spectra					2173:2179	the matrix and biofilm biomass spectra	2142:2179	spectra	2173:2179	The difference between the matrix and biofilm biomass spectra showed successful matrix extraction rather than simply the presence of cell debris after sonication.
31293526	14	10	theme	debris	2257:2262	arg1	presence					2240:2247	simply the presence	2229:2247	successful matrix extraction rather than simply the presence of cell debris	2188:2262	The difference between the matrix and biofilm biomass spectra showed successful matrix extraction rather than simply the presence of cell debris after sonication.
31293526	13	11	theme	scattering	2036:2045	arg1	profiles					2054:2061	the surface-enhanced Raman scattering (SERS) profiles	2009:2061	the surface-enhanced Raman scattering (SERS) profiles of the C. acnes RT5 biofilm matrix and biofilm biomass	2009:2116	We also performed surface-enhanced Raman spectroscopy analysis of the C. acnes RT5 matrix for the first time, providing the surface-enhanced Raman scattering (SERS) profiles of the C. acnes RT5 biofilm matrix and biofilm biomass.
31293526	10	12	theme	1→4	1471:1473	arg1	-β-GalpNAc-					1536:1546	→6)-α-D-Galp-(1→4)-β-D-ManpNAc3NAcA-(1→6)-α-D-Glcp-(1→4)-β-D-ManpNAc3NAcA-(1→3)-β-GalpNAc-	1457:1546	→6)-α-D-Galp-(1→4)-β-D-ManpNAc3NAcA-(1→6)-α-D-Glcp-(1→4)-β-D-ManpNAc3NAcA-(1→3)-β-GalpNAc-(1→	1457:1549	The chemical structure of the major polysaccharide was determined using a nuclear magnetic resonance technique, the formula being →6)-α-D-Galp-(1→4)-β-D-ManpNAc3NAcA-(1→6)-α-D-Glcp-(1→4)-β-D-ManpNAc3NAcA-(1→3)-β-GalpNAc-(1→.
31293526	10	13	theme	-α-D-Galp-	1460:1469	arg1	-β-GalpNAc-					1536:1546	→6)-α-D-Galp-(1→4)-β-D-ManpNAc3NAcA-(1→6)-α-D-Glcp-(1→4)-β-D-ManpNAc3NAcA-(1→3)-β-GalpNAc-	1457:1546	→6)-α-D-Galp-(1→4)-β-D-ManpNAc3NAcA-(1→6)-α-D-Glcp-(1→4)-β-D-ManpNAc3NAcA-(1→3)-β-GalpNAc-(1→	1457:1549	The chemical structure of the major polysaccharide was determined using a nuclear magnetic resonance technique, the formula being →6)-α-D-Galp-(1→4)-β-D-ManpNAc3NAcA-(1→6)-α-D-Glcp-(1→4)-β-D-ManpNAc3NAcA-(1→3)-β-GalpNAc-(1→.
31293526	13	14	theme	biofilm	2102:2108	arg1	biomass					2110:2116	the C. acnes RT5 biofilm matrix and biofilm biomass	2066:2116	biomass	2110:2116	We also performed surface-enhanced Raman spectroscopy analysis of the C. acnes RT5 matrix for the first time, providing the surface-enhanced Raman scattering (SERS) profiles of the C. acnes RT5 biofilm matrix and biofilm biomass.
31293526	10	15	theme	1→6	1494:1496	arg1	-β-GalpNAc-					1536:1546	→6)-α-D-Galp-(1→4)-β-D-ManpNAc3NAcA-(1→6)-α-D-Glcp-(1→4)-β-D-ManpNAc3NAcA-(1→3)-β-GalpNAc-	1457:1546	→6)-α-D-Galp-(1→4)-β-D-ManpNAc3NAcA-(1→6)-α-D-Glcp-(1→4)-β-D-ManpNAc3NAcA-(1→3)-β-GalpNAc-(1→	1457:1549	The chemical structure of the major polysaccharide was determined using a nuclear magnetic resonance technique, the formula being →6)-α-D-Galp-(1→4)-β-D-ManpNAc3NAcA-(1→6)-α-D-Glcp-(1→4)-β-D-ManpNAc3NAcA-(1→3)-β-GalpNAc-(1→.
31293526	5	16	theme	bacteria	685:692	arg1	matrix					661:666	the biofilm matrix	649:666	the biofilm matrix of Gram-positive bacteria	649:692	Here, we developed a new technique for the extraction of the biofilm matrix of Gram-positive bacteria without the use of chemical or enzymatic digestion, known to be a source of artifacts.
31293526	12	17	contain	contained	1765:1773	arg2	hydrolases					1788:1797	more than 20 hydrolases	1775:1797	more than 20 hydrolases of various substrata, pathogenicity factors, and many intracellular proteins and enzymes	1775:1886	The matrix also contained more than 20 hydrolases of various substrata, pathogenicity factors, and many intracellular proteins and enzymes.
31293526	12	17	contain	contained	1765:1773	arg1	matrix					1753:1758	The matrix	1749:1758	The matrix	1749:1758	The matrix also contained more than 20 hydrolases of various substrata, pathogenicity factors, and many intracellular proteins and enzymes.
31293526	12	18	theme	various	1802:1808	arg1	substrata					1810:1818	various substrata	1802:1818	various substrata	1802:1818	The matrix also contained more than 20 hydrolases of various substrata, pathogenicity factors, and many intracellular proteins and enzymes.
31293526	9	19	dep	%	1274:1274	arg1	porphyrins					1293:1302	porphyrins precursors and other	1293:1323	porphyrins	1293:1302	The following is the ratio of the main matrix components: 62.6% polysaccharides, 9.6% proteins, 4.0% DNA, and 23.8% other compounds (porphyrins precursors and other).
31293526	9	19	dep	%	1274:1274	arg1	other					1319:1323	other	1319:1323	other	1319:1323	The following is the ratio of the main matrix components: 62.6% polysaccharides, 9.6% proteins, 4.0% DNA, and 23.8% other compounds (porphyrins precursors and other).
31293526	9	19	dep	%	1274:1274	arg1	compounds					1282:1290	other compounds	1276:1290	23.8% other compounds (porphyrins precursors and other)	1270:1324	The following is the ratio of the main matrix components: 62.6% polysaccharides, 9.6% proteins, 4.0% DNA, and 23.8% other compounds (porphyrins precursors and other).
31293526	10	20	theme	-β-D-ManpNAc3NAcA-	1475:1492	arg1	-β-GalpNAc-					1536:1546	→6)-α-D-Galp-(1→4)-β-D-ManpNAc3NAcA-(1→6)-α-D-Glcp-(1→4)-β-D-ManpNAc3NAcA-(1→3)-β-GalpNAc-	1457:1546	→6)-α-D-Galp-(1→4)-β-D-ManpNAc3NAcA-(1→6)-α-D-Glcp-(1→4)-β-D-ManpNAc3NAcA-(1→3)-β-GalpNAc-(1→	1457:1549	The chemical structure of the major polysaccharide was determined using a nuclear magnetic resonance technique, the formula being →6)-α-D-Galp-(1→4)-β-D-ManpNAc3NAcA-(1→6)-α-D-Glcp-(1→4)-β-D-ManpNAc3NAcA-(1→3)-β-GalpNAc-(1→.
31293526	13	21	theme	RT5	2079:2081	arg1	matrix					2091:2096	the C. acnes RT5 biofilm matrix and biofilm biomass	2066:2116	matrix	2091:2096	We also performed surface-enhanced Raman spectroscopy analysis of the C. acnes RT5 matrix for the first time, providing the surface-enhanced Raman scattering (SERS) profiles of the C. acnes RT5 biofilm matrix and biofilm biomass.
31293526	6	22	theme	biofilms	865:872	arg1	matrix					845:850	matrix	845:850	matrix	845:850	Our method is based on the physical separation of the cells and matrix of sonicated biofilms by ultracentrifugation through a CsCl gradient.
31293526	6	22	theme	biofilms	865:872	arg1	cells					835:839	cells	835:839	cells	835:839	Our method is based on the physical separation of the cells and matrix of sonicated biofilms by ultracentrifugation through a CsCl gradient.
31293526	11	23	from	matrix	1584:1589	arg1	abundant					1610:1617	abundant	1610:1617	abundant	1610:1617	We detected 447 proteins in the matrix, of which the most abundant were the chaperonin GroL, the elongation factors EF-Tu and EF-G, several enzymes of glycolysis, and proteins of unknown function.
31293526	11	23	from	matrix	1584:1589	arg1	GroL					1639:1642	the chaperonin GroL	1624:1642	the chaperonin GroL	1624:1642	We detected 447 proteins in the matrix, of which the most abundant were the chaperonin GroL, the elongation factors EF-Tu and EF-G, several enzymes of glycolysis, and proteins of unknown function.
31293526	7	24	theme	filters	978:984	arg1	surface					949:955	the surface	945:955	the surface of cellulose acetate filters	945:984	Biofilms were grown on the surface of cellulose acetate filters, and the biomass was collected without contamination by the growth medium.
31293526	13	25	theme	Raman	1924:1928	arg1	analysis					1943:1950	surface-enhanced Raman spectroscopy analysis	1907:1950	surface-enhanced Raman spectroscopy analysis of the C. acnes RT5 matrix	1907:1977	We also performed surface-enhanced Raman spectroscopy analysis of the C. acnes RT5 matrix for the first time, providing the surface-enhanced Raman scattering (SERS) profiles of the C. acnes RT5 biofilm matrix and biofilm biomass.
31293526	1	26	theme	Propionibacterium	113:129	arg1	acnes					99:103	Cutibacterium acnes	85:103	Cutibacterium acnes (former Propionibacterium acnes)	85:136	In skin, Cutibacterium acnes (former Propionibacterium acnes) can behave as an opportunistic pathogen, depending on the strain and environmental conditions.
31293526	1	26	theme	Propionibacterium	113:129	arg1	acnes					131:135	former Propionibacterium acnes	106:135	former Propionibacterium acnes	106:135	In skin, Cutibacterium acnes (former Propionibacterium acnes) can behave as an opportunistic pathogen, depending on the strain and environmental conditions.
31293526	13	27	theme	biofilm	2083:2089	arg1	matrix					2091:2096	the C. acnes RT5 biofilm matrix and biofilm biomass	2066:2116	matrix	2091:2096	We also performed surface-enhanced Raman spectroscopy analysis of the C. acnes RT5 matrix for the first time, providing the surface-enhanced Raman scattering (SERS) profiles of the C. acnes RT5 biofilm matrix and biofilm biomass.
31293526	9	28	dep	porphyrins	1293:1302	arg1	precursors					1304:1313	precursors	1304:1313	precursors	1304:1313	The following is the ratio of the main matrix components: 62.6% polysaccharides, 9.6% proteins, 4.0% DNA, and 23.8% other compounds (porphyrins precursors and other).
31293526	12	29	theme	intracellular	1853:1865	arg1	proteins					1867:1874	many intracellular proteins	1848:1874	many intracellular proteins	1848:1874	The matrix also contained more than 20 hydrolases of various substrata, pathogenicity factors, and many intracellular proteins and enzymes.
31293526	0	30	theme	Acneic	57:62	arg1	RT5					71:73	Acneic Strain RT5	57:73	Acneic Strain RT5	57:73	Composition of the Biofilm Matrix of Cutibacterium acnes Acneic Strain RT5.
31293526	9	31	theme	matrix	1199:1204	arg1	components					1206:1215	the main matrix components	1190:1215	the main matrix components	1190:1215	The following is the ratio of the main matrix components: 62.6% polysaccharides, 9.6% proteins, 4.0% DNA, and 23.8% other compounds (porphyrins precursors and other).
31293526	5	32	theme	biofilm	653:659	arg1	matrix					661:666	the biofilm matrix	649:666	the biofilm matrix of Gram-positive bacteria	649:692	Here, we developed a new technique for the extraction of the biofilm matrix of Gram-positive bacteria without the use of chemical or enzymatic digestion, known to be a source of artifacts.
31293526	5	33	theme	digestion	735:743	arg1	use					706:708	the use	702:708	the use of chemical or enzymatic digestion	702:743	Here, we developed a new technique for the extraction of the biofilm matrix of Gram-positive bacteria without the use of chemical or enzymatic digestion, known to be a source of artifacts.
31293526	13	34	theme	C.	2070:2071	arg1	matrix					2091:2096	the C. acnes RT5 biofilm matrix and biofilm biomass	2066:2116	matrix	2091:2096	We also performed surface-enhanced Raman spectroscopy analysis of the C. acnes RT5 matrix for the first time, providing the surface-enhanced Raman scattering (SERS) profiles of the C. acnes RT5 biofilm matrix and biofilm biomass.
31293526	9	35	theme	%	1222:1222	arg1	polysaccharides					1224:1238	62.6% polysaccharides	1218:1238	62.6% polysaccharides	1218:1238	The following is the ratio of the main matrix components: 62.6% polysaccharides, 9.6% proteins, 4.0% DNA, and 23.8% other compounds (porphyrins precursors and other).
31293526	15	36	theme	matrix	2328:2333	arg1	composition					2335:2345	the biofilm matrix composition	2316:2345	the biofilm matrix composition	2316:2345	These data show the complexity of the biofilm matrix composition and should be essential for the development of new anti-C.
31293526	13	37	theme	matrix	2091:2096	arg1	profiles					2054:2061	the surface-enhanced Raman scattering (SERS) profiles	2009:2061	the surface-enhanced Raman scattering (SERS) profiles of the C. acnes RT5 biofilm matrix and biofilm biomass	2009:2116	We also performed surface-enhanced Raman spectroscopy analysis of the C. acnes RT5 matrix for the first time, providing the surface-enhanced Raman scattering (SERS) profiles of the C. acnes RT5 biofilm matrix and biofilm biomass.
31293526	3	38	theme	essential	454:462	arg1	functions					474:482	essential bacterial functions	454:482	essential bacterial functions	454:482	The essential exogenous products of C. acnes accumulate in the extracellular matrix of the biofilm, conferring essential bacterial functions to this structure.
31293526	0	39	theme	Strain	64:69	arg1	RT5					71:73	Acneic Strain RT5	57:73	Acneic Strain RT5	57:73	Composition of the Biofilm Matrix of Cutibacterium acnes Acneic Strain RT5.
31293526	3	40	theme	extracellular	406:418	arg1	matrix					420:425	the extracellular matrix	402:425	the extracellular matrix of the biofilm	402:440	The essential exogenous products of C. acnes accumulate in the extracellular matrix of the biofilm, conferring essential bacterial functions to this structure.
31293526	9	41	theme	%	1244:1244	arg1	proteins					1246:1253	9.6% proteins	1241:1253	9.6% proteins	1241:1253	The following is the ratio of the main matrix components: 62.6% polysaccharides, 9.6% proteins, 4.0% DNA, and 23.8% other compounds (porphyrins precursors and other).
31293526	13	42	theme	spectroscopy	1930:1941	arg1	analysis					1943:1950	surface-enhanced Raman spectroscopy analysis	1907:1950	surface-enhanced Raman spectroscopy analysis of the C. acnes RT5 matrix	1907:1977	We also performed surface-enhanced Raman spectroscopy analysis of the C. acnes RT5 matrix for the first time, providing the surface-enhanced Raman scattering (SERS) profiles of the C. acnes RT5 biofilm matrix and biofilm biomass.
31293526	3	43	theme	exogenous	357:365	arg1	products					367:374	The essential exogenous products	343:374	The essential exogenous products of C. acnes	343:386	The essential exogenous products of C. acnes accumulate in the extracellular matrix of the biofilm, conferring essential bacterial functions to this structure.
31293526	11	44	theme	function	1739:1746	arg1	factors					1660:1666	the elongation factors	1645:1666	the elongation factors EF-Tu and EF-G	1645:1681	We detected 447 proteins in the matrix, of which the most abundant were the chaperonin GroL, the elongation factors EF-Tu and EF-G, several enzymes of glycolysis, and proteins of unknown function.
31293526	11	44	theme	function	1739:1746	arg1	EF-Tu					1668:1672	EF-Tu	1668:1672	EF-Tu	1668:1672	We detected 447 proteins in the matrix, of which the most abundant were the chaperonin GroL, the elongation factors EF-Tu and EF-G, several enzymes of glycolysis, and proteins of unknown function.
31293526	11	44	theme	function	1739:1746	arg1	proteins					1568:1575	447 proteins	1564:1575	447 proteins	1564:1575	We detected 447 proteins in the matrix, of which the most abundant were the chaperonin GroL, the elongation factors EF-Tu and EF-G, several enzymes of glycolysis, and proteins of unknown function.
31293526	11	44	theme	function	1739:1746	arg1	EF-G					1678:1681	EF-G	1678:1681	EF-G	1678:1681	We detected 447 proteins in the matrix, of which the most abundant were the chaperonin GroL, the elongation factors EF-Tu and EF-G, several enzymes of glycolysis, and proteins of unknown function.
31293526	11	44	theme	function	1739:1746	arg1	proteins					1719:1726	proteins	1719:1726	proteins of unknown function	1719:1746	We detected 447 proteins in the matrix, of which the most abundant were the chaperonin GroL, the elongation factors EF-Tu and EF-G, several enzymes of glycolysis, and proteins of unknown function.
31293526	11	44	theme	function	1739:1746	arg1	enzymes					1692:1698	several enzymes	1684:1698	several enzymes of glycolysis	1684:1712	We detected 447 proteins in the matrix, of which the most abundant were the chaperonin GroL, the elongation factors EF-Tu and EF-G, several enzymes of glycolysis, and proteins of unknown function.
31293526	5	45	theme	artifacts	770:778	arg1	source					760:765	a source	758:765	a source of artifacts	758:778	Here, we developed a new technique for the extraction of the biofilm matrix of Gram-positive bacteria without the use of chemical or enzymatic digestion, known to be a source of artifacts.
31293526	6	46	theme	physical	808:815	arg1	separation					817:826	the physical separation	804:826	the physical separation of the cells and matrix of sonicated biofilms by ultracentrifugation through a CsCl gradient	804:919	Our method is based on the physical separation of the cells and matrix of sonicated biofilms by ultracentrifugation through a CsCl gradient.
31293526	10	47	theme	polysaccharide	1363:1376	arg1	structure					1340:1348	The chemical structure	1327:1348	The chemical structure of the major polysaccharide	1327:1376	The chemical structure of the major polysaccharide was determined using a nuclear magnetic resonance technique, the formula being →6)-α-D-Galp-(1→4)-β-D-ManpNAc3NAcA-(1→6)-α-D-Glcp-(1→4)-β-D-ManpNAc3NAcA-(1→3)-β-GalpNAc-(1→.
31293526	3	48	theme	biofilm	434:440	arg1	matrix					420:425	the extracellular matrix	402:425	the extracellular matrix of the biofilm	402:440	The essential exogenous products of C. acnes accumulate in the extracellular matrix of the biofilm, conferring essential bacterial functions to this structure.
31293526	0	49	theme	Matrix	27:32	arg1	Composition					0:10	Composition	0:10	Composition of the Biofilm Matrix of Cutibacterium	0:49	Composition of the Biofilm Matrix of Cutibacterium acnes Acneic Strain RT5.
31293526	9	50	theme	%	1259:1259	arg1	DNA					1261:1263	4.0% DNA	1256:1263	4.0% DNA	1256:1263	The following is the ratio of the main matrix components: 62.6% polysaccharides, 9.6% proteins, 4.0% DNA, and 23.8% other compounds (porphyrins precursors and other).
31293526	12	51	theme	substrata	1810:1818	arg1	hydrolases					1788:1797	more than 20 hydrolases	1775:1797	more than 20 hydrolases of various substrata, pathogenicity factors, and many intracellular proteins and enzymes	1775:1886	The matrix also contained more than 20 hydrolases of various substrata, pathogenicity factors, and many intracellular proteins and enzymes.
31293526	3	52	theme	acnes	382:386	arg1	products					367:374	The essential exogenous products	343:374	The essential exogenous products of C. acnes	343:386	The essential exogenous products of C. acnes accumulate in the extracellular matrix of the biofilm, conferring essential bacterial functions to this structure.
31293526	10	53	theme	magnetic	1409:1416	arg1	technique					1428:1436	a nuclear magnetic resonance technique	1399:1436	a nuclear magnetic resonance technique	1399:1436	The chemical structure of the major polysaccharide was determined using a nuclear magnetic resonance technique, the formula being →6)-α-D-Galp-(1→4)-β-D-ManpNAc3NAcA-(1→6)-α-D-Glcp-(1→4)-β-D-ManpNAc3NAcA-(1→3)-β-GalpNAc-(1→.
31293526	10	53	theme	magnetic	1409:1416	arg1	formula					1443:1449	the formula	1439:1449	the formula being →6)-α-D-Galp-(1→4)-β-D-ManpNAc3NAcA-(1→6)-α-D-Glcp-(1→4)-β-D-ManpNAc3NAcA-(1→3)-β-GalpNAc-(1→	1439:1549	The chemical structure of the major polysaccharide was determined using a nuclear magnetic resonance technique, the formula being →6)-α-D-Galp-(1→4)-β-D-ManpNAc3NAcA-(1→6)-α-D-Glcp-(1→4)-β-D-ManpNAc3NAcA-(1→3)-β-GalpNAc-(1→.
31293526	2	54	theme	skin	327:330	arg1	disorders					332:340	skin disorders	327:340	skin disorders	327:340	Acneic strains of C. acnes form biofilms inside skin-gland hollows, inducing inflammation and skin disorders.
31293526	0	55	theme	Cutibacterium	37:49	arg1	Matrix					27:32	the Biofilm Matrix	15:32	the Biofilm Matrix of Cutibacterium	15:49	Composition of the Biofilm Matrix of Cutibacterium acnes Acneic Strain RT5.
31293526	13	56	theme	surface-enhanced	2013:2028	arg1	SERS					2048:2051	SERS	2048:2051	SERS	2048:2051	We also performed surface-enhanced Raman spectroscopy analysis of the C. acnes RT5 matrix for the first time, providing the surface-enhanced Raman scattering (SERS) profiles of the C. acnes RT5 biofilm matrix and biofilm biomass.
31293526	13	56	theme	surface-enhanced	2013:2028	arg1	scattering					2036:2045	surface-enhanced Raman scattering	2013:2045	the surface-enhanced Raman scattering (SERS) profiles of the C. acnes RT5 biofilm matrix and biofilm biomass	2009:2116	We also performed surface-enhanced Raman spectroscopy analysis of the C. acnes RT5 matrix for the first time, providing the surface-enhanced Raman scattering (SERS) profiles of the C. acnes RT5 biofilm matrix and biofilm biomass.
31293526	11	57	theme	proteins	1568:1575	arg1	abundant					1610:1617	abundant	1610:1617	abundant	1610:1617	We detected 447 proteins in the matrix, of which the most abundant were the chaperonin GroL, the elongation factors EF-Tu and EF-G, several enzymes of glycolysis, and proteins of unknown function.
31293526	11	57	theme	proteins	1568:1575	arg1	GroL					1639:1642	the chaperonin GroL	1624:1642	the chaperonin GroL	1624:1642	We detected 447 proteins in the matrix, of which the most abundant were the chaperonin GroL, the elongation factors EF-Tu and EF-G, several enzymes of glycolysis, and proteins of unknown function.
31293526	3	58	theme	bacterial	464:472	arg1	functions					474:482	essential bacterial functions	454:482	essential bacterial functions	454:482	The essential exogenous products of C. acnes accumulate in the extracellular matrix of the biofilm, conferring essential bacterial functions to this structure.
31293526	15	59	theme	anti-C	2398:2403	arg1	development					2379:2389	the development	2375:2389	the development of new anti-C	2375:2403	These data show the complexity of the biofilm matrix composition and should be essential for the development of new anti-C.
31293526	4	60	theme	C.	582:583	arg1	acnes					585:589	C. acnes	582:589	C. acnes	582:589	However, little is known about the actual composition of the biofilm matrix of C. acnes.
31293526	11	61	theme	unknown	1731:1737	arg1	function					1739:1746	unknown function	1731:1746	unknown function	1731:1746	We detected 447 proteins in the matrix, of which the most abundant were the chaperonin GroL, the elongation factors EF-Tu and EF-G, several enzymes of glycolysis, and proteins of unknown function.
31293526	11	62	theme	enzymes	1692:1698	arg1	abundant					1610:1617	abundant	1610:1617	abundant	1610:1617	We detected 447 proteins in the matrix, of which the most abundant were the chaperonin GroL, the elongation factors EF-Tu and EF-G, several enzymes of glycolysis, and proteins of unknown function.
31293526	11	62	theme	enzymes	1692:1698	arg1	GroL					1639:1642	the chaperonin GroL	1624:1642	the chaperonin GroL	1624:1642	We detected 447 proteins in the matrix, of which the most abundant were the chaperonin GroL, the elongation factors EF-Tu and EF-G, several enzymes of glycolysis, and proteins of unknown function.
31293526	9	63	theme	other	1276:1280	arg1	porphyrins					1293:1302	porphyrins precursors and other	1293:1323	porphyrins	1293:1302	The following is the ratio of the main matrix components: 62.6% polysaccharides, 9.6% proteins, 4.0% DNA, and 23.8% other compounds (porphyrins precursors and other).
31293526	9	63	theme	other	1276:1280	arg1	other					1319:1323	other	1319:1323	other	1319:1323	The following is the ratio of the main matrix components: 62.6% polysaccharides, 9.6% proteins, 4.0% DNA, and 23.8% other compounds (porphyrins precursors and other).
31293526	9	63	theme	other	1276:1280	arg1	compounds					1282:1290	other compounds	1276:1290	23.8% other compounds (porphyrins precursors and other)	1270:1324	The following is the ratio of the main matrix components: 62.6% polysaccharides, 9.6% proteins, 4.0% DNA, and 23.8% other compounds (porphyrins precursors and other).
31293526	8	64	theme	biofilm	1065:1071	arg1	matrix					1073:1078	The biofilm matrix	1061:1078	The biofilm matrix of the acneic C. acnes RT5 strain	1061:1112	The biofilm matrix of the acneic C. acnes RT5 strain appears to consist mainly of polysaccharides.
31293526	6	65	theme	sonicated	855:863	arg1	biofilms					865:872	sonicated biofilms	855:872	sonicated biofilms	855:872	Our method is based on the physical separation of the cells and matrix of sonicated biofilms by ultracentrifugation through a CsCl gradient.
31293526	5	66	theme	new	613:615	arg1	technique					617:625	a new technique	611:625	a new technique	611:625	Here, we developed a new technique for the extraction of the biofilm matrix of Gram-positive bacteria without the use of chemical or enzymatic digestion, known to be a source of artifacts.
31293526	13	67	theme	acnes	1962:1966	arg1	matrix					1972:1977	the C. acnes RT5 matrix	1955:1977	the C. acnes RT5 matrix	1955:1977	We also performed surface-enhanced Raman spectroscopy analysis of the C. acnes RT5 matrix for the first time, providing the surface-enhanced Raman scattering (SERS) profiles of the C. acnes RT5 biofilm matrix and biofilm biomass.
31293526	6	68	theme	matrix	845:850	arg1	separation					817:826	the physical separation	804:826	the physical separation of the cells and matrix of sonicated biofilms by ultracentrifugation through a CsCl gradient	804:919	Our method is based on the physical separation of the cells and matrix of sonicated biofilms by ultracentrifugation through a CsCl gradient.
31293526	7	69	theme	growth	1046:1051	arg1	medium					1053:1058	the growth medium	1042:1058	the growth medium	1042:1058	Biofilms were grown on the surface of cellulose acetate filters, and the biomass was collected without contamination by the growth medium.
31293526	14	70	theme	successful	2188:2197	arg1	extraction					2206:2215	successful matrix extraction	2188:2215	successful matrix extraction rather than simply the presence of cell debris	2188:2262	The difference between the matrix and biofilm biomass spectra showed successful matrix extraction rather than simply the presence of cell debris after sonication.
31293526	11	71	from	proteins	1719:1726	arg1	matrix					1584:1589	the matrix	1580:1589	the matrix	1580:1589	We detected 447 proteins in the matrix, of which the most abundant were the chaperonin GroL, the elongation factors EF-Tu and EF-G, several enzymes of glycolysis, and proteins of unknown function.
31293526	13	72	theme	matrix	1972:1977	arg1	analysis					1943:1950	surface-enhanced Raman spectroscopy analysis	1907:1950	surface-enhanced Raman spectroscopy analysis of the C. acnes RT5 matrix	1907:1977	We also performed surface-enhanced Raman spectroscopy analysis of the C. acnes RT5 matrix for the first time, providing the surface-enhanced Raman scattering (SERS) profiles of the C. acnes RT5 biofilm matrix and biofilm biomass.
31293526	8	73	theme	acneic	1087:1092	arg1	strain					1107:1112	the acneic C. acnes RT5 strain	1083:1112	the acneic C. acnes RT5 strain	1083:1112	The biofilm matrix of the acneic C. acnes RT5 strain appears to consist mainly of polysaccharides.
31293526	6	74	theme	cells	835:839	arg1	separation					817:826	the physical separation	804:826	the physical separation of the cells and matrix of sonicated biofilms by ultracentrifugation through a CsCl gradient	804:919	Our method is based on the physical separation of the cells and matrix of sonicated biofilms by ultracentrifugation through a CsCl gradient.
31293526	4	75	theme	matrix	572:577	arg1	composition					545:555	the actual composition	534:555	the actual composition of the biofilm matrix of C. acnes	534:589	However, little is known about the actual composition of the biofilm matrix of C. acnes.
31293526	2	76	theme	acnes	254:258	arg1	strains					240:246	Acneic strains	233:246	Acneic strains of C. acnes	233:258	Acneic strains of C. acnes form biofilms inside skin-gland hollows, inducing inflammation and skin disorders.
31293526	8	77	theme	acnes	1097:1101	arg1	strain					1107:1112	the acneic C. acnes RT5 strain	1083:1112	the acneic C. acnes RT5 strain	1083:1112	The biofilm matrix of the acneic C. acnes RT5 strain appears to consist mainly of polysaccharides.
31293526	14	78	theme	biofilm	2157:2163	arg1	spectra					2173:2179	the matrix and biofilm biomass spectra	2142:2179	spectra	2173:2179	The difference between the matrix and biofilm biomass spectra showed successful matrix extraction rather than simply the presence of cell debris after sonication.
31293526	1	79	theme	opportunistic	155:167	arg1	pathogen					169:176	an opportunistic pathogen	152:176	an opportunistic pathogen	152:176	In skin, Cutibacterium acnes (former Propionibacterium acnes) can behave as an opportunistic pathogen, depending on the strain and environmental conditions.
31293526	8	80	theme	strain	1107:1112	arg1	matrix					1073:1078	The biofilm matrix	1061:1078	The biofilm matrix of the acneic C. acnes RT5 strain	1061:1112	The biofilm matrix of the acneic C. acnes RT5 strain appears to consist mainly of polysaccharides.
31293526	16	81	theme	acnes	2406:2410	arg1	biofilms					2412:2419	acnes biofilms	2406:2419	acnes biofilms	2406:2419	acnes biofilms and potential antibiofilm drugs.
31293526	11	82	theme	chaperonin	1628:1637	arg1	abundant					1610:1617	abundant	1610:1617	abundant	1610:1617	We detected 447 proteins in the matrix, of which the most abundant were the chaperonin GroL, the elongation factors EF-Tu and EF-G, several enzymes of glycolysis, and proteins of unknown function.
31293526	11	82	theme	chaperonin	1628:1637	arg1	GroL					1639:1642	the chaperonin GroL	1624:1642	the chaperonin GroL	1624:1642	We detected 447 proteins in the matrix, of which the most abundant were the chaperonin GroL, the elongation factors EF-Tu and EF-G, several enzymes of glycolysis, and proteins of unknown function.
31293526	5	83	theme	matrix	661:666	arg1	extraction					635:644	the extraction	631:644	the extraction of the biofilm matrix of Gram-positive bacteria without the use of chemical or enzymatic digestion	631:743	Here, we developed a new technique for the extraction of the biofilm matrix of Gram-positive bacteria without the use of chemical or enzymatic digestion, known to be a source of artifacts.
31293526	2	84	theme	skin-gland	281:290	arg1	hollows					292:298	skin-gland hollows	281:298	skin-gland hollows	281:298	Acneic strains of C. acnes form biofilms inside skin-gland hollows, inducing inflammation and skin disorders.
31293526	6	85	theme	CsCl	907:910	arg1	gradient					912:919	a CsCl gradient	905:919	a CsCl gradient	905:919	Our method is based on the physical separation of the cells and matrix of sonicated biofilms by ultracentrifugation through a CsCl gradient.
31293526	5	86	theme	Gram-positive	671:683	arg1	bacteria					685:692	Gram-positive bacteria	671:692	Gram-positive bacteria	671:692	Here, we developed a new technique for the extraction of the biofilm matrix of Gram-positive bacteria without the use of chemical or enzymatic digestion, known to be a source of artifacts.
31293526	13	87	theme	biomass	2110:2116	arg1	profiles					2054:2061	the surface-enhanced Raman scattering (SERS) profiles	2009:2061	the surface-enhanced Raman scattering (SERS) profiles of the C. acnes RT5 biofilm matrix and biofilm biomass	2009:2116	We also performed surface-enhanced Raman spectroscopy analysis of the C. acnes RT5 matrix for the first time, providing the surface-enhanced Raman scattering (SERS) profiles of the C. acnes RT5 biofilm matrix and biofilm biomass.
31293526	16	88	theme	antibiofilm	2435:2445	arg1	drugs					2447:2451	potential antibiofilm drugs	2425:2451	potential antibiofilm drugs	2425:2451	acnes biofilms and potential antibiofilm drugs.
31293526	1	89	dep	strain	196:201	arg1	the					192:194	the	192:194	the	192:194	In skin, Cutibacterium acnes (former Propionibacterium acnes) can behave as an opportunistic pathogen, depending on the strain and environmental conditions.
31293526	11	90	theme	elongation	1649:1658	arg1	factors					1660:1666	the elongation factors	1645:1666	the elongation factors EF-Tu and EF-G	1645:1681	We detected 447 proteins in the matrix, of which the most abundant were the chaperonin GroL, the elongation factors EF-Tu and EF-G, several enzymes of glycolysis, and proteins of unknown function.
31293526	11	90	theme	elongation	1649:1658	arg1	EF-G					1678:1681	EF-G	1678:1681	EF-G	1678:1681	We detected 447 proteins in the matrix, of which the most abundant were the chaperonin GroL, the elongation factors EF-Tu and EF-G, several enzymes of glycolysis, and proteins of unknown function.
31293526	11	90	theme	elongation	1649:1658	arg1	EF-Tu					1668:1672	EF-Tu	1668:1672	EF-Tu	1668:1672	We detected 447 proteins in the matrix, of which the most abundant were the chaperonin GroL, the elongation factors EF-Tu and EF-G, several enzymes of glycolysis, and proteins of unknown function.
31293526	13	91	theme	C.	1959:1960	arg1	matrix					1972:1977	the C. acnes RT5 matrix	1955:1977	the C. acnes RT5 matrix	1955:1977	We also performed surface-enhanced Raman spectroscopy analysis of the C. acnes RT5 matrix for the first time, providing the surface-enhanced Raman scattering (SERS) profiles of the C. acnes RT5 biofilm matrix and biofilm biomass.
31293526	7	92	theme	cellulose	960:968	arg1	filters					978:984	cellulose acetate filters	960:984	cellulose acetate filters	960:984	Biofilms were grown on the surface of cellulose acetate filters, and the biomass was collected without contamination by the growth medium.
31293526	13	93	theme	acnes	2073:2077	arg1	matrix					2091:2096	the C. acnes RT5 biofilm matrix and biofilm biomass	2066:2116	matrix	2091:2096	We also performed surface-enhanced Raman spectroscopy analysis of the C. acnes RT5 matrix for the first time, providing the surface-enhanced Raman scattering (SERS) profiles of the C. acnes RT5 biofilm matrix and biofilm biomass.
31293526	7	94	theme	acetate	970:976	arg1	filters					978:984	cellulose acetate filters	960:984	cellulose acetate filters	960:984	Biofilms were grown on the surface of cellulose acetate filters, and the biomass was collected without contamination by the growth medium.
31293526	13	95	theme	surface-enhanced	1907:1922	arg1	analysis					1943:1950	surface-enhanced Raman spectroscopy analysis	1907:1950	surface-enhanced Raman spectroscopy analysis of the C. acnes RT5 matrix	1907:1977	We also performed surface-enhanced Raman spectroscopy analysis of the C. acnes RT5 matrix for the first time, providing the surface-enhanced Raman scattering (SERS) profiles of the C. acnes RT5 biofilm matrix and biofilm biomass.
31293526	1	96	theme	former	106:111	arg1	acnes					99:103	Cutibacterium acnes	85:103	Cutibacterium acnes (former Propionibacterium acnes)	85:136	In skin, Cutibacterium acnes (former Propionibacterium acnes) can behave as an opportunistic pathogen, depending on the strain and environmental conditions.
31293526	1	96	theme	former	106:111	arg1	acnes					131:135	former Propionibacterium acnes	106:135	former Propionibacterium acnes	106:135	In skin, Cutibacterium acnes (former Propionibacterium acnes) can behave as an opportunistic pathogen, depending on the strain and environmental conditions.
31293526	11	97	theme	factors	1660:1666	arg1	abundant					1610:1617	abundant	1610:1617	abundant	1610:1617	We detected 447 proteins in the matrix, of which the most abundant were the chaperonin GroL, the elongation factors EF-Tu and EF-G, several enzymes of glycolysis, and proteins of unknown function.
31293526	11	97	theme	factors	1660:1666	arg1	GroL					1639:1642	the chaperonin GroL	1624:1642	the chaperonin GroL	1624:1642	We detected 447 proteins in the matrix, of which the most abundant were the chaperonin GroL, the elongation factors EF-Tu and EF-G, several enzymes of glycolysis, and proteins of unknown function.
31293526	5	98	theme	chemical	713:720	arg1	digestion					735:743	chemical or enzymatic digestion	713:743	chemical or enzymatic digestion	713:743	Here, we developed a new technique for the extraction of the biofilm matrix of Gram-positive bacteria without the use of chemical or enzymatic digestion, known to be a source of artifacts.
31293526	9	99	theme	main	1194:1197	arg1	components					1206:1215	the main matrix components	1190:1215	the main matrix components	1190:1215	The following is the ratio of the main matrix components: 62.6% polysaccharides, 9.6% proteins, 4.0% DNA, and 23.8% other compounds (porphyrins precursors and other).
31293526	2	100	theme	Acneic	233:238	arg1	strains					240:246	Acneic strains	233:246	Acneic strains of C. acnes	233:258	Acneic strains of C. acnes form biofilms inside skin-gland hollows, inducing inflammation and skin disorders.
31293526	12	101	theme	many	1848:1851	arg1	proteins					1867:1874	many intracellular proteins	1848:1874	many intracellular proteins	1848:1874	The matrix also contained more than 20 hydrolases of various substrata, pathogenicity factors, and many intracellular proteins and enzymes.
31293526	5	102	theme	enzymatic	725:733	arg1	digestion					735:743	chemical or enzymatic digestion	713:743	chemical or enzymatic digestion	713:743	Here, we developed a new technique for the extraction of the biofilm matrix of Gram-positive bacteria without the use of chemical or enzymatic digestion, known to be a source of artifacts.
31293526	9	103	theme	components	1206:1215	arg1	following					1164:1172	The following	1160:1172	The following	1160:1172	The following is the ratio of the main matrix components: 62.6% polysaccharides, 9.6% proteins, 4.0% DNA, and 23.8% other compounds (porphyrins precursors and other).
31293526	9	103	theme	components	1206:1215	arg1	ratio					1181:1185	the ratio	1177:1185	the ratio of the main matrix components: 62.6% polysaccharides, 9.6% proteins, 4.0% DNA, and 23.8% other compounds (porphyrins precursors and other)	1177:1324	The following is the ratio of the main matrix components: 62.6% polysaccharides, 9.6% proteins, 4.0% DNA, and 23.8% other compounds (porphyrins precursors and other).
31293526	11	104	from	proteins	1568:1575	arg1	matrix					1584:1589	the matrix	1580:1589	the matrix	1580:1589	We detected 447 proteins in the matrix, of which the most abundant were the chaperonin GroL, the elongation factors EF-Tu and EF-G, several enzymes of glycolysis, and proteins of unknown function.
31293526	12	105	theme	proteins	1867:1874	arg1	hydrolases					1788:1797	more than 20 hydrolases	1775:1797	more than 20 hydrolases of various substrata, pathogenicity factors, and many intracellular proteins and enzymes	1775:1886	The matrix also contained more than 20 hydrolases of various substrata, pathogenicity factors, and many intracellular proteins and enzymes.
31293526	9	106	theme	62.6	1218:1221	arg1	%					1222:1222	%	1222:1222	%	1222:1222	The following is the ratio of the main matrix components: 62.6% polysaccharides, 9.6% proteins, 4.0% DNA, and 23.8% other compounds (porphyrins precursors and other).
31293526	11	107	dep	factors	1660:1666	arg1	factors					1660:1666	the elongation factors	1645:1666	the elongation factors EF-Tu and EF-G	1645:1681	We detected 447 proteins in the matrix, of which the most abundant were the chaperonin GroL, the elongation factors EF-Tu and EF-G, several enzymes of glycolysis, and proteins of unknown function.
31293526	11	107	dep	factors	1660:1666	arg1	EF-G					1678:1681	EF-G	1678:1681	EF-G	1678:1681	We detected 447 proteins in the matrix, of which the most abundant were the chaperonin GroL, the elongation factors EF-Tu and EF-G, several enzymes of glycolysis, and proteins of unknown function.
31293526	11	107	dep	factors	1660:1666	arg1	EF-Tu					1668:1672	EF-Tu	1668:1672	EF-Tu	1668:1672	We detected 447 proteins in the matrix, of which the most abundant were the chaperonin GroL, the elongation factors EF-Tu and EF-G, several enzymes of glycolysis, and proteins of unknown function.
31293526	3	108	theme	essential	347:355	arg1	products					367:374	The essential exogenous products	343:374	The essential exogenous products of C. acnes	343:386	The essential exogenous products of C. acnes accumulate in the extracellular matrix of the biofilm, conferring essential bacterial functions to this structure.
31293526	11	109	from	enzymes	1692:1698	arg1	matrix					1584:1589	the matrix	1580:1589	the matrix	1580:1589	We detected 447 proteins in the matrix, of which the most abundant were the chaperonin GroL, the elongation factors EF-Tu and EF-G, several enzymes of glycolysis, and proteins of unknown function.
31293526	9	110	theme	polysaccharides	1224:1238	arg1	following					1164:1172	The following	1160:1172	The following	1160:1172	The following is the ratio of the main matrix components: 62.6% polysaccharides, 9.6% proteins, 4.0% DNA, and 23.8% other compounds (porphyrins precursors and other).
31293526	9	110	theme	polysaccharides	1224:1238	arg1	ratio					1181:1185	the ratio	1177:1185	the ratio of the main matrix components: 62.6% polysaccharides, 9.6% proteins, 4.0% DNA, and 23.8% other compounds (porphyrins precursors and other)	1177:1324	The following is the ratio of the main matrix components: 62.6% polysaccharides, 9.6% proteins, 4.0% DNA, and 23.8% other compounds (porphyrins precursors and other).
31293526	15	111	theme	composition	2335:2345	arg1	complexity					2302:2311	the complexity	2298:2311	the complexity of the biofilm matrix composition	2298:2345	These data show the complexity of the biofilm matrix composition and should be essential for the development of new anti-C.
31293526	11	112	from	factors	1660:1666	arg1	matrix					1584:1589	the matrix	1580:1589	the matrix	1580:1589	We detected 447 proteins in the matrix, of which the most abundant were the chaperonin GroL, the elongation factors EF-Tu and EF-G, several enzymes of glycolysis, and proteins of unknown function.
31293526	13	113	theme	first	1987:1991	arg1	time					1993:1996	the first time	1983:1996	the first time	1983:1996	We also performed surface-enhanced Raman spectroscopy analysis of the C. acnes RT5 matrix for the first time, providing the surface-enhanced Raman scattering (SERS) profiles of the C. acnes RT5 biofilm matrix and biofilm biomass.
31293526	10	114	theme	1→4	1509:1511	arg1	-β-GalpNAc-					1536:1546	→6)-α-D-Galp-(1→4)-β-D-ManpNAc3NAcA-(1→6)-α-D-Glcp-(1→4)-β-D-ManpNAc3NAcA-(1→3)-β-GalpNAc-	1457:1546	→6)-α-D-Galp-(1→4)-β-D-ManpNAc3NAcA-(1→6)-α-D-Glcp-(1→4)-β-D-ManpNAc3NAcA-(1→3)-β-GalpNAc-(1→	1457:1549	The chemical structure of the major polysaccharide was determined using a nuclear magnetic resonance technique, the formula being →6)-α-D-Galp-(1→4)-β-D-ManpNAc3NAcA-(1→6)-α-D-Glcp-(1→4)-β-D-ManpNAc3NAcA-(1→3)-β-GalpNAc-(1→.
31293526	9	115	theme	9.6	1241:1243	arg1	%					1244:1244	%	1244:1244	%	1244:1244	The following is the ratio of the main matrix components: 62.6% polysaccharides, 9.6% proteins, 4.0% DNA, and 23.8% other compounds (porphyrins precursors and other).
31293526	15	116	theme	biofilm	2320:2326	arg1	composition					2335:2345	the biofilm matrix composition	2316:2345	the biofilm matrix composition	2316:2345	These data show the complexity of the biofilm matrix composition and should be essential for the development of new anti-C.
31293526	4	117	theme	actual	538:543	arg1	composition					545:555	the actual composition	534:555	the actual composition of the biofilm matrix of C. acnes	534:589	However, little is known about the actual composition of the biofilm matrix of C. acnes.
31293526	10	118	theme	chemical	1331:1338	arg1	structure					1340:1348	The chemical structure	1327:1348	The chemical structure of the major polysaccharide	1327:1376	The chemical structure of the major polysaccharide was determined using a nuclear magnetic resonance technique, the formula being →6)-α-D-Galp-(1→4)-β-D-ManpNAc3NAcA-(1→6)-α-D-Glcp-(1→4)-β-D-ManpNAc3NAcA-(1→3)-β-GalpNAc-(1→.
31293526	10	119	dep	-β-GalpNAc-	1536:1546	arg1	1→					1548:1549	1→	1548:1549	→6)-α-D-Galp-(1→4)-β-D-ManpNAc3NAcA-(1→6)-α-D-Glcp-(1→4)-β-D-ManpNAc3NAcA-(1→3)-β-GalpNAc-(1→	1457:1549	The chemical structure of the major polysaccharide was determined using a nuclear magnetic resonance technique, the formula being →6)-α-D-Galp-(1→4)-β-D-ManpNAc3NAcA-(1→6)-α-D-Glcp-(1→4)-β-D-ManpNAc3NAcA-(1→3)-β-GalpNAc-(1→.
31293526	10	120	theme	-α-D-Glcp-	1498:1507	arg1	-β-GalpNAc-					1536:1546	→6)-α-D-Galp-(1→4)-β-D-ManpNAc3NAcA-(1→6)-α-D-Glcp-(1→4)-β-D-ManpNAc3NAcA-(1→3)-β-GalpNAc-	1457:1546	→6)-α-D-Galp-(1→4)-β-D-ManpNAc3NAcA-(1→6)-α-D-Glcp-(1→4)-β-D-ManpNAc3NAcA-(1→3)-β-GalpNAc-(1→	1457:1549	The chemical structure of the major polysaccharide was determined using a nuclear magnetic resonance technique, the formula being →6)-α-D-Galp-(1→4)-β-D-ManpNAc3NAcA-(1→6)-α-D-Glcp-(1→4)-β-D-ManpNAc3NAcA-(1→3)-β-GalpNAc-(1→.
31293526	0	121	theme	Biofilm	19:25	arg1	Matrix					27:32	the Biofilm Matrix	15:32	the Biofilm Matrix of Cutibacterium	15:49	Composition of the Biofilm Matrix of Cutibacterium acnes Acneic Strain RT5.
31293526	9	122	theme	proteins	1246:1253	arg1	following					1164:1172	The following	1160:1172	The following	1160:1172	The following is the ratio of the main matrix components: 62.6% polysaccharides, 9.6% proteins, 4.0% DNA, and 23.8% other compounds (porphyrins precursors and other).
31293526	9	122	theme	proteins	1246:1253	arg1	ratio					1181:1185	the ratio	1177:1185	the ratio of the main matrix components: 62.6% polysaccharides, 9.6% proteins, 4.0% DNA, and 23.8% other compounds (porphyrins precursors and other)	1177:1324	The following is the ratio of the main matrix components: 62.6% polysaccharides, 9.6% proteins, 4.0% DNA, and 23.8% other compounds (porphyrins precursors and other).
31293526	10	123	theme	1→3	1532:1534	arg1	-β-GalpNAc-					1536:1546	→6)-α-D-Galp-(1→4)-β-D-ManpNAc3NAcA-(1→6)-α-D-Glcp-(1→4)-β-D-ManpNAc3NAcA-(1→3)-β-GalpNAc-	1457:1546	→6)-α-D-Galp-(1→4)-β-D-ManpNAc3NAcA-(1→6)-α-D-Glcp-(1→4)-β-D-ManpNAc3NAcA-(1→3)-β-GalpNAc-(1→	1457:1549	The chemical structure of the major polysaccharide was determined using a nuclear magnetic resonance technique, the formula being →6)-α-D-Galp-(1→4)-β-D-ManpNAc3NAcA-(1→6)-α-D-Glcp-(1→4)-β-D-ManpNAc3NAcA-(1→3)-β-GalpNAc-(1→.
31293526	9	124	theme	4.0	1256:1258	arg1	%					1259:1259	%	1259:1259	%	1259:1259	The following is the ratio of the main matrix components: 62.6% polysaccharides, 9.6% proteins, 4.0% DNA, and 23.8% other compounds (porphyrins precursors and other).
31293526	10	125	theme	major	1357:1361	arg1	polysaccharide					1363:1376	the major polysaccharide	1353:1376	the major polysaccharide	1353:1376	The chemical structure of the major polysaccharide was determined using a nuclear magnetic resonance technique, the formula being →6)-α-D-Galp-(1→4)-β-D-ManpNAc3NAcA-(1→6)-α-D-Glcp-(1→4)-β-D-ManpNAc3NAcA-(1→3)-β-GalpNAc-(1→.
31293526	1	126	theme	environmental	207:219	arg1	conditions					221:230	environmental conditions	207:230	environmental conditions	207:230	In skin, Cutibacterium acnes (former Propionibacterium acnes) can behave as an opportunistic pathogen, depending on the strain and environmental conditions.
31293526	14	127	theme	cell	2252:2255	arg1	debris					2257:2262	cell debris	2252:2262	cell debris	2252:2262	The difference between the matrix and biofilm biomass spectra showed successful matrix extraction rather than simply the presence of cell debris after sonication.
31293526	10	128	theme	-β-D-ManpNAc3NAcA-	1513:1530	arg1	-β-GalpNAc-					1536:1546	→6)-α-D-Galp-(1→4)-β-D-ManpNAc3NAcA-(1→6)-α-D-Glcp-(1→4)-β-D-ManpNAc3NAcA-(1→3)-β-GalpNAc-	1457:1546	→6)-α-D-Galp-(1→4)-β-D-ManpNAc3NAcA-(1→6)-α-D-Glcp-(1→4)-β-D-ManpNAc3NAcA-(1→3)-β-GalpNAc-(1→	1457:1549	The chemical structure of the major polysaccharide was determined using a nuclear magnetic resonance technique, the formula being →6)-α-D-Galp-(1→4)-β-D-ManpNAc3NAcA-(1→6)-α-D-Glcp-(1→4)-β-D-ManpNAc3NAcA-(1→3)-β-GalpNAc-(1→.
31293526	9	129	theme	DNA	1261:1263	arg1	following					1164:1172	The following	1160:1172	The following	1160:1172	The following is the ratio of the main matrix components: 62.6% polysaccharides, 9.6% proteins, 4.0% DNA, and 23.8% other compounds (porphyrins precursors and other).
31293526	9	129	theme	DNA	1261:1263	arg1	ratio					1181:1185	the ratio	1177:1185	the ratio of the main matrix components: 62.6% polysaccharides, 9.6% proteins, 4.0% DNA, and 23.8% other compounds (porphyrins precursors and other)	1177:1324	The following is the ratio of the main matrix components: 62.6% polysaccharides, 9.6% proteins, 4.0% DNA, and 23.8% other compounds (porphyrins precursors and other).
31293526	12	130	theme	pathogenicity	1821:1833	arg1	factors					1835:1841	pathogenicity factors	1821:1841	pathogenicity factors	1821:1841	The matrix also contained more than 20 hydrolases of various substrata, pathogenicity factors, and many intracellular proteins and enzymes.
31293526	10	131	theme	nuclear	1401:1407	arg1	technique					1428:1436	a nuclear magnetic resonance technique	1399:1436	a nuclear magnetic resonance technique	1399:1436	The chemical structure of the major polysaccharide was determined using a nuclear magnetic resonance technique, the formula being →6)-α-D-Galp-(1→4)-β-D-ManpNAc3NAcA-(1→6)-α-D-Glcp-(1→4)-β-D-ManpNAc3NAcA-(1→3)-β-GalpNAc-(1→.
31293526	10	131	theme	nuclear	1401:1407	arg1	formula					1443:1449	the formula	1439:1449	the formula being →6)-α-D-Galp-(1→4)-β-D-ManpNAc3NAcA-(1→6)-α-D-Glcp-(1→4)-β-D-ManpNAc3NAcA-(1→3)-β-GalpNAc-(1→	1439:1549	The chemical structure of the major polysaccharide was determined using a nuclear magnetic resonance technique, the formula being →6)-α-D-Galp-(1→4)-β-D-ManpNAc3NAcA-(1→6)-α-D-Glcp-(1→4)-β-D-ManpNAc3NAcA-(1→3)-β-GalpNAc-(1→.
31293526	15	132	theme	new	2394:2396	arg1	anti-C					2398:2403	new anti-C	2394:2403	new anti-C	2394:2403	These data show the complexity of the biofilm matrix composition and should be essential for the development of new anti-C.
31293526	13	133	theme	Raman	2030:2034	arg1	SERS					2048:2051	SERS	2048:2051	SERS	2048:2051	We also performed surface-enhanced Raman spectroscopy analysis of the C. acnes RT5 matrix for the first time, providing the surface-enhanced Raman scattering (SERS) profiles of the C. acnes RT5 biofilm matrix and biofilm biomass.
31293526	13	133	theme	Raman	2030:2034	arg1	scattering					2036:2045	surface-enhanced Raman scattering	2013:2045	the surface-enhanced Raman scattering (SERS) profiles of the C. acnes RT5 biofilm matrix and biofilm biomass	2009:2116	We also performed surface-enhanced Raman spectroscopy analysis of the C. acnes RT5 matrix for the first time, providing the surface-enhanced Raman scattering (SERS) profiles of the C. acnes RT5 biofilm matrix and biofilm biomass.
31293526	4	134	theme	acnes	585:589	arg1	matrix					572:577	the biofilm matrix	560:577	the biofilm matrix of C. acnes	560:589	However, little is known about the actual composition of the biofilm matrix of C. acnes.
31293526	9	135	theme	%	1274:1274	arg1	following					1164:1172	The following	1160:1172	The following	1160:1172	The following is the ratio of the main matrix components: 62.6% polysaccharides, 9.6% proteins, 4.0% DNA, and 23.8% other compounds (porphyrins precursors and other).
31293526	9	135	theme	%	1274:1274	arg1	ratio					1181:1185	the ratio	1177:1185	the ratio of the main matrix components: 62.6% polysaccharides, 9.6% proteins, 4.0% DNA, and 23.8% other compounds (porphyrins precursors and other)	1177:1324	The following is the ratio of the main matrix components: 62.6% polysaccharides, 9.6% proteins, 4.0% DNA, and 23.8% other compounds (porphyrins precursors and other).
31293526	12	136	theme	factors	1835:1841	arg1	hydrolases					1788:1797	more than 20 hydrolases	1775:1797	more than 20 hydrolases of various substrata, pathogenicity factors, and many intracellular proteins and enzymes	1775:1886	The matrix also contained more than 20 hydrolases of various substrata, pathogenicity factors, and many intracellular proteins and enzymes.
31293526	11	137	theme	proteins	1719:1726	arg1	abundant					1610:1617	abundant	1610:1617	abundant	1610:1617	We detected 447 proteins in the matrix, of which the most abundant were the chaperonin GroL, the elongation factors EF-Tu and EF-G, several enzymes of glycolysis, and proteins of unknown function.
31293526	11	137	theme	proteins	1719:1726	arg1	GroL					1639:1642	the chaperonin GroL	1624:1642	the chaperonin GroL	1624:1642	We detected 447 proteins in the matrix, of which the most abundant were the chaperonin GroL, the elongation factors EF-Tu and EF-G, several enzymes of glycolysis, and proteins of unknown function.
31293526	6	138	dep	cells	835:839	arg1	the					831:833	the	831:833	the	831:833	Our method is based on the physical separation of the cells and matrix of sonicated biofilms by ultracentrifugation through a CsCl gradient.
31293526	16	139	theme	potential	2425:2433	arg1	drugs					2447:2451	potential antibiofilm drugs	2425:2451	potential antibiofilm drugs	2425:2451	acnes biofilms and potential antibiofilm drugs.
32047483	0	0	theme	Milk	68:71	arg1	Viscosity					45:53	Viscosity	45:53	Viscosity of Fermented Milk	45:71	Ability of a Wild Weissella Strain to Modify Viscosity of Fermented Milk.
32047483	4	1	theme	rotational	606:615	arg1	viscometer					617:626	one rotational viscometer	602:626	one rotational viscometer	602:626	To reach this goal, milk viscosity has been evaluated by means of two very different instruments: one rotational viscometer and the Ford cup.
32047483	4	1	theme	rotational	606:615	arg1	instruments					589:599	two very different instruments	570:599	two very different instruments: one rotational viscometer and the Ford cup	570:643	To reach this goal, milk viscosity has been evaluated by means of two very different instruments: one rotational viscometer and the Ford cup.
32047483	0	2	theme	Fermented	58:66	arg1	Milk					68:71	Fermented Milk	58:71	Fermented Milk	58:71	Ability of a Wild Weissella Strain to Modify Viscosity of Fermented Milk.
32047483	6	3	theme	milk	815:818	arg1	viscosity					820:828	milk viscosity	815:828	milk viscosity	815:828	W4451, previously isolated from sourdough, was able to acidify milk, to produce polysaccharides in situ and thus improve milk viscosity.
32047483	1	4	theme	new	224:226	arg1	perspectives					228:239	new perspectives	224:239	new perspectives on their potential employment	224:269	Despite the fact that strains belonging to Weissella species have not yet been approved for use as starter culture, recent toxicological studies open new perspectives on their potential employment.
32047483	4	5	theme	milk	524:527	arg1	viscosity					529:537	milk viscosity	524:537	milk viscosity	524:537	To reach this goal, milk viscosity has been evaluated by means of two very different instruments: one rotational viscometer and the Ford cup.
32047483	7	6	theme	dairy	997:1001	arg1	products					1003:1010	dairy products	997:1010	dairy products	997:1010	The ability of W4451 to produce at the same time lactic acid and high amounts of polysaccharides makes it a good candidate to improve the composition of starters for dairy products.
32047483	3	7	theme	dairy	488:492	arg1	products					494:501	dairy products	488:501	dairy products	488:501	bulgaricus, which is commonly used for this purpose in dairy products.
32047483	5	8	theme	water	656:660	arg1	capacity					670:677	water holding capacity	656:677	water holding capacity	656:677	Moreover, water holding capacity was evaluated.
32047483	8	9	theme	simple	1041:1046	arg1	method					1048:1053	a simple method	1039:1053	a simple method to measure fermented milk viscosity by small- or medium-sized dairies	1039:1123	Ford cup turned out to be a simple method to measure fermented milk viscosity by small- or medium-sized dairies.
32047483	8	9	theme	simple	1041:1046	arg1	cup					1018:1020	Ford cup	1013:1020	Ford cup	1013:1020	Ford cup turned out to be a simple method to measure fermented milk viscosity by small- or medium-sized dairies.
32047483	4	10	theme	different	579:587	arg1	viscometer					617:626	one rotational viscometer	602:626	one rotational viscometer	602:626	To reach this goal, milk viscosity has been evaluated by means of two very different instruments: one rotational viscometer and the Ford cup.
32047483	4	10	theme	different	579:587	arg1	cup					641:643	the Ford cup	632:643	the Ford cup	632:643	To reach this goal, milk viscosity has been evaluated by means of two very different instruments: one rotational viscometer and the Ford cup.
32047483	4	10	theme	different	579:587	arg1	instruments					589:599	two very different instruments	570:599	two very different instruments: one rotational viscometer and the Ford cup	570:643	To reach this goal, milk viscosity has been evaluated by means of two very different instruments: one rotational viscometer and the Ford cup.
32047483	5	11	theme	holding	662:668	arg1	capacity					670:677	water holding capacity	656:677	water holding capacity	656:677	Moreover, water holding capacity was evaluated.
32047483	7	12	theme	starters	984:991	arg1	composition					969:979	the composition	965:979	the composition of starters for dairy products	965:1010	The ability of W4451 to produce at the same time lactic acid and high amounts of polysaccharides makes it a good candidate to improve the composition of starters for dairy products.
32047483	2	13	theme	minor	342:346	arg1	strain					356:361	a wild Weissella minor (W4451) strain	325:361	a wild Weissella minor (W4451) strain	325:361	The aim of this study was to evaluate the ability of a wild Weissella minor (W4451) strain to modify milk viscosity compared to Lactobacillus delbrueckii subsp.
32047483	7	14	theme	W4451	846:850	arg1	ability					835:841	The ability	831:841	The ability of W4451 to produce at the same time lactic acid and high amounts of polysaccharides	831:926	The ability of W4451 to produce at the same time lactic acid and high amounts of polysaccharides makes it a good candidate to improve the composition of starters for dairy products.
32047483	2	15	theme	Weissella	332:340	arg1	strain					356:361	a wild Weissella minor (W4451) strain	325:361	a wild Weissella minor (W4451) strain	325:361	The aim of this study was to evaluate the ability of a wild Weissella minor (W4451) strain to modify milk viscosity compared to Lactobacillus delbrueckii subsp.
32047483	8	16	theme	medium-sized	1104:1115	arg1	dairies					1117:1123	small- or medium-sized dairies	1094:1123	small- or medium-sized dairies	1094:1123	Ford cup turned out to be a simple method to measure fermented milk viscosity by small- or medium-sized dairies.
32047483	2	17	theme	wild	327:330	arg1	strain					356:361	a wild Weissella minor (W4451) strain	325:361	a wild Weissella minor (W4451) strain	325:361	The aim of this study was to evaluate the ability of a wild Weissella minor (W4451) strain to modify milk viscosity compared to Lactobacillus delbrueckii subsp.
32047483	0	18	theme	Wild	13:16	arg1	Strain					28:33	a Wild Weissella Strain	11:33	a Wild Weissella Strain	11:33	Ability of a Wild Weissella Strain to Modify Viscosity of Fermented Milk.
32047483	1	19	theme	potential	250:258	arg1	employment					260:269	their potential employment	244:269	their potential employment	244:269	Despite the fact that strains belonging to Weissella species have not yet been approved for use as starter culture, recent toxicological studies open new perspectives on their potential employment.
32047483	1	20	from	perspectives	228:239	arg1	employment					260:269	their potential employment	244:269	their potential employment	244:269	Despite the fact that strains belonging to Weissella species have not yet been approved for use as starter culture, recent toxicological studies open new perspectives on their potential employment.
32047483	8	21	theme	fermented	1066:1074	arg1	viscosity					1081:1089	fermented milk viscosity	1066:1089	fermented milk viscosity	1066:1089	Ford cup turned out to be a simple method to measure fermented milk viscosity by small- or medium-sized dairies.
32047483	2	22	theme	strain	356:361	arg1	ability					314:320	the ability	310:320	the ability of a wild Weissella minor (W4451) strain to modify milk viscosity compared to Lactobacillus delbrueckii subsp	310:430	The aim of this study was to evaluate the ability of a wild Weissella minor (W4451) strain to modify milk viscosity compared to Lactobacillus delbrueckii subsp.
32047483	0	23	theme	Strain	28:33	arg1	Ability					0:6	Ability	0:6	Ability of a Wild Weissella Strain	0:33	Ability of a Wild Weissella Strain to Modify Viscosity of Fermented Milk.
32047483	7	24	theme	good	939:942	arg1	candidate					944:952	a good candidate	937:952	a good candidate to improve the composition of starters for dairy products	937:1010	The ability of W4451 to produce at the same time lactic acid and high amounts of polysaccharides makes it a good candidate to improve the composition of starters for dairy products.
32047483	1	25	theme	starter	173:179	arg1	culture					181:187	starter culture	173:187	starter culture	173:187	Despite the fact that strains belonging to Weissella species have not yet been approved for use as starter culture, recent toxicological studies open new perspectives on their potential employment.
32047483	2	26	theme	delbrueckii	414:424	arg1	subsp					426:430	Lactobacillus delbrueckii subsp	400:430	Lactobacillus delbrueckii subsp	400:430	The aim of this study was to evaluate the ability of a wild Weissella minor (W4451) strain to modify milk viscosity compared to Lactobacillus delbrueckii subsp.
32047483	0	27	theme	Weissella	18:26	arg1	Strain					28:33	a Wild Weissella Strain	11:33	a Wild Weissella Strain	11:33	Ability of a Wild Weissella Strain to Modify Viscosity of Fermented Milk.
32047483	2	28	theme	milk	373:376	arg1	viscosity					378:386	milk viscosity	373:386	milk viscosity	373:386	The aim of this study was to evaluate the ability of a wild Weissella minor (W4451) strain to modify milk viscosity compared to Lactobacillus delbrueckii subsp.
32047483	2	29	theme	W4451	349:353	arg1	strain					356:361	a wild Weissella minor (W4451) strain	325:361	a wild Weissella minor (W4451) strain	325:361	The aim of this study was to evaluate the ability of a wild Weissella minor (W4451) strain to modify milk viscosity compared to Lactobacillus delbrueckii subsp.
32047483	2	30	theme	Lactobacillus	400:412	arg1	subsp					426:430	Lactobacillus delbrueckii subsp	400:430	Lactobacillus delbrueckii subsp	400:430	The aim of this study was to evaluate the ability of a wild Weissella minor (W4451) strain to modify milk viscosity compared to Lactobacillus delbrueckii subsp.
32047483	8	31	theme	milk	1076:1079	arg1	viscosity					1081:1089	fermented milk viscosity	1066:1089	fermented milk viscosity	1066:1089	Ford cup turned out to be a simple method to measure fermented milk viscosity by small- or medium-sized dairies.
32047483	8	32	theme	Ford	1013:1016	arg1	method					1048:1053	a simple method	1039:1053	a simple method to measure fermented milk viscosity by small- or medium-sized dairies	1039:1123	Ford cup turned out to be a simple method to measure fermented milk viscosity by small- or medium-sized dairies.
32047483	8	32	theme	Ford	1013:1016	arg1	cup					1018:1020	Ford cup	1013:1020	Ford cup	1013:1020	Ford cup turned out to be a simple method to measure fermented milk viscosity by small- or medium-sized dairies.
32047483	4	33	theme	Ford	636:639	arg1	instruments					589:599	two very different instruments	570:599	two very different instruments: one rotational viscometer and the Ford cup	570:643	To reach this goal, milk viscosity has been evaluated by means of two very different instruments: one rotational viscometer and the Ford cup.
32047483	4	33	theme	Ford	636:639	arg1	cup					641:643	the Ford cup	632:643	the Ford cup	632:643	To reach this goal, milk viscosity has been evaluated by means of two very different instruments: one rotational viscometer and the Ford cup.
32047483	6	34	dep	produce	766:772	arg1	improve					807:813	improve	807:813	improve milk viscosity	807:828	W4451, previously isolated from sourdough, was able to acidify milk, to produce polysaccharides in situ and thus improve milk viscosity.
32047483	7	35	theme	polysaccharides	912:926	arg1	polysaccharides					912:926	polysaccharides	912:926	polysaccharides	912:926	The ability of W4451 to produce at the same time lactic acid and high amounts of polysaccharides makes it a good candidate to improve the composition of starters for dairy products.
32047483	7	35	theme	polysaccharides	912:926	arg1	acid					887:890	lactic acid	880:890	lactic acid	880:890	The ability of W4451 to produce at the same time lactic acid and high amounts of polysaccharides makes it a good candidate to improve the composition of starters for dairy products.
32047483	7	35	theme	polysaccharides	912:926	arg1	amounts					901:907	high amounts	896:907	high amounts of polysaccharides	896:926	The ability of W4451 to produce at the same time lactic acid and high amounts of polysaccharides makes it a good candidate to improve the composition of starters for dairy products.
32047483	7	36	theme	high	896:899	arg1	polysaccharides					912:926	polysaccharides	912:926	polysaccharides	912:926	The ability of W4451 to produce at the same time lactic acid and high amounts of polysaccharides makes it a good candidate to improve the composition of starters for dairy products.
32047483	7	36	theme	high	896:899	arg1	amounts					901:907	high amounts	896:907	high amounts of polysaccharides	896:926	The ability of W4451 to produce at the same time lactic acid and high amounts of polysaccharides makes it a good candidate to improve the composition of starters for dairy products.
32047483	4	37	dep	instruments	589:599	arg1	viscometer					617:626	one rotational viscometer	602:626	one rotational viscometer	602:626	To reach this goal, milk viscosity has been evaluated by means of two very different instruments: one rotational viscometer and the Ford cup.
32047483	4	37	dep	instruments	589:599	arg1	instruments					589:599	two very different instruments	570:599	two very different instruments: one rotational viscometer and the Ford cup	570:643	To reach this goal, milk viscosity has been evaluated by means of two very different instruments: one rotational viscometer and the Ford cup.
32047483	4	37	dep	instruments	589:599	arg1	cup					641:643	the Ford cup	632:643	the Ford cup	632:643	To reach this goal, milk viscosity has been evaluated by means of two very different instruments: one rotational viscometer and the Ford cup.
32047483	7	38	theme	lactic	880:885	arg1	acid					887:890	lactic acid	880:890	lactic acid	880:890	The ability of W4451 to produce at the same time lactic acid and high amounts of polysaccharides makes it a good candidate to improve the composition of starters for dairy products.
32047483	2	39	theme	study	288:292	arg1	aim					276:278	The aim	272:278	The aim of this study	272:292	The aim of this study was to evaluate the ability of a wild Weissella minor (W4451) strain to modify milk viscosity compared to Lactobacillus delbrueckii subsp.
32047483	8	40	theme	small-	1094:1099	arg1	dairies					1117:1123	small- or medium-sized dairies	1094:1123	small- or medium-sized dairies	1094:1123	Ford cup turned out to be a simple method to measure fermented milk viscosity by small- or medium-sized dairies.
32047483	1	41	theme	recent	190:195	arg1	studies					211:217	recent toxicological studies	190:217	recent toxicological studies	190:217	Despite the fact that strains belonging to Weissella species have not yet been approved for use as starter culture, recent toxicological studies open new perspectives on their potential employment.
32047483	1	42	theme	Weissella	117:125	arg1	species					127:133	Weissella species	117:133	Weissella species	117:133	Despite the fact that strains belonging to Weissella species have not yet been approved for use as starter culture, recent toxicological studies open new perspectives on their potential employment.
32047483	1	43	theme	toxicological	197:209	arg1	studies					211:217	recent toxicological studies	190:217	recent toxicological studies	190:217	Despite the fact that strains belonging to Weissella species have not yet been approved for use as starter culture, recent toxicological studies open new perspectives on their potential employment.
32047483	6	44	attach	isolated	712:719	arg2	W4451					694:698	W4451	694:698	W4451	694:698	W4451, previously isolated from sourdough, was able to acidify milk, to produce polysaccharides in situ and thus improve milk viscosity.
32047483	6	44	attach	isolated	712:719	arg1	sourdough					726:734	sourdough	726:734	sourdough	726:734	W4451, previously isolated from sourdough, was able to acidify milk, to produce polysaccharides in situ and thus improve milk viscosity.
32047483	3	45	used	used	463:466	arg2	which					445:449	which	445:449	which	445:449	bulgaricus, which is commonly used for this purpose in dairy products.
32047483	7	46	theme	same	870:873	arg1	time					875:878	the same time	866:878	the same time	866:878	The ability of W4451 to produce at the same time lactic acid and high amounts of polysaccharides makes it a good candidate to improve the composition of starters for dairy products.
31116255	2	0	theme	ethanol	523:529	arg1	administration					532:545	intragastric liquor [50% (v/v) ethanol] administration	492:545	intragastric liquor [50% (v/v) ethanol] administration at 10 mL/kg body weight for 20 days	492:581	ALD rat models were established by intragastric liquor [50% (v/v) ethanol] administration at 10 mL/kg body weight for 20 days.
31116255	2	0	theme	ethanol	523:529	arg1	v/v					518:520	v/v	518:520	v/v	518:520	ALD rat models were established by intragastric liquor [50% (v/v) ethanol] administration at 10 mL/kg body weight for 20 days.
31116255	10	1	theme	occludin	1548:1555	arg1	expression					1534:1543	the expression	1530:1543	the expression of occludin and claudin-1 in intestine tissue	1530:1589	Administration of LREE suppressed TLR4 overexpression and promoted the expression of occludin and claudin-1 in intestine tissue.
31116255	6	2	theme	gut	997:999	arg1	composition					1012:1022	The gut microbiota composition	993:1022	The gut microbiota composition	993:1022	The gut microbiota composition was evaluated by 16S rDNA sequencing.
31116255	3	3	theme	normal	619:624	arg1	treatment					636:644	no treatment	633:644	no treatment	633:644	Rats were divided into six groups: normal group (no treatment), model group (ALD rats), Essentiale group (ALD rats fed with Essentiale, 137 mg/kg), and LREE high/moderate/low dose groups (ALD rats fed with 4, 2, or 1 g LREE/kg).
31116255	3	3	theme	normal	619:624	arg1	groups					611:616	six groups	607:616	six groups: normal group (no treatment), model group (ALD rats), Essentiale group (ALD rats fed with Essentiale, 137 mg/kg), and LREE high/moderate/low dose groups	607:769	Rats were divided into six groups: normal group (no treatment), model group (ALD rats), Essentiale group (ALD rats fed with Essentiale, 137 mg/kg), and LREE high/moderate/low dose groups (ALD rats fed with 4, 2, or 1 g LREE/kg).
31116255	3	3	theme	normal	619:624	arg1	group					626:630	normal group	619:630	normal group (no treatment)	619:645	Rats were divided into six groups: normal group (no treatment), model group (ALD rats), Essentiale group (ALD rats fed with Essentiale, 137 mg/kg), and LREE high/moderate/low dose groups (ALD rats fed with 4, 2, or 1 g LREE/kg).
31116255	1	4	theme	alcoholic	257:265	arg1	ALD					282:284	ALD	282:284	ALD	282:284	This study aimed to explore the influence of gut microbiota alterations induced by Linderae radix ethanol extract (LREE) on alcoholic liver disease (ALD) in rats and to study the anti-inflammatory effect of LREE on ALD through the lipopolysaccharide (LPS) toll-like receptor 4 (TLR4)-nuclear factor kappa B (NF-κB) pathway.
31116255	1	4	theme	alcoholic	257:265	arg1	disease					273:279	alcoholic liver disease	257:279	alcoholic liver disease (ALD) in rats	257:293	This study aimed to explore the influence of gut microbiota alterations induced by Linderae radix ethanol extract (LREE) on alcoholic liver disease (ALD) in rats and to study the anti-inflammatory effect of LREE on ALD through the lipopolysaccharide (LPS) toll-like receptor 4 (TLR4)-nuclear factor kappa B (NF-κB) pathway.
31116255	3	5	theme	g	801:801	arg1	LREE/kg					803:809	4, 2, or 1 g LREE/kg	790:809	4, 2, or 1 g LREE/kg	790:809	Rats were divided into six groups: normal group (no treatment), model group (ALD rats), Essentiale group (ALD rats fed with Essentiale, 137 mg/kg), and LREE high/moderate/low dose groups (ALD rats fed with 4, 2, or 1 g LREE/kg).
31116255	10	6	theme	intestine	1574:1582	arg1	tissue					1584:1589	intestine tissue	1574:1589	intestine tissue	1574:1589	Administration of LREE suppressed TLR4 overexpression and promoted the expression of occludin and claudin-1 in intestine tissue.
31116255	1	7	from	disease	273:279	arg1	rats					290:293	rats	290:293	rats	290:293	This study aimed to explore the influence of gut microbiota alterations induced by Linderae radix ethanol extract (LREE) on alcoholic liver disease (ALD) in rats and to study the anti-inflammatory effect of LREE on ALD through the lipopolysaccharide (LPS) toll-like receptor 4 (TLR4)-nuclear factor kappa B (NF-κB) pathway.
31116255	1	8	theme	factor	425:430	arg1	NF-κB					441:445	NF-κB	441:445	NF-κB	441:445	This study aimed to explore the influence of gut microbiota alterations induced by Linderae radix ethanol extract (LREE) on alcoholic liver disease (ALD) in rats and to study the anti-inflammatory effect of LREE on ALD through the lipopolysaccharide (LPS) toll-like receptor 4 (TLR4)-nuclear factor kappa B (NF-κB) pathway.
31116255	1	8	theme	factor	425:430	arg1	B					438:438	lipopolysaccharide (LPS) toll-like receptor 4 (TLR4)-nuclear factor kappa B	364:438	the lipopolysaccharide (LPS) toll-like receptor 4 (TLR4)-nuclear factor kappa B (NF-κB) pathway	360:454	This study aimed to explore the influence of gut microbiota alterations induced by Linderae radix ethanol extract (LREE) on alcoholic liver disease (ALD) in rats and to study the anti-inflammatory effect of LREE on ALD through the lipopolysaccharide (LPS) toll-like receptor 4 (TLR4)-nuclear factor kappa B (NF-κB) pathway.
31116255	1	9	theme	microbiota	182:191	arg1	alterations					193:203	gut microbiota alterations	178:203	gut microbiota alterations induced by Linderae radix ethanol extract (LREE)	178:252	This study aimed to explore the influence of gut microbiota alterations induced by Linderae radix ethanol extract (LREE) on alcoholic liver disease (ALD) in rats and to study the anti-inflammatory effect of LREE on ALD through the lipopolysaccharide (LPS) toll-like receptor 4 (TLR4)-nuclear factor kappa B (NF-κB) pathway.
31116255	5	10	theme	Liver	850:854	arg1	changes					869:875	Liver pathological changes	850:875	Liver pathological changes	850:875	Liver pathological changes and intestinal ultrastructure were examined by hematoxylin and eosin staining and transmission electron microscopy.
31116255	8	11	theme	pathological	1251:1262	arg1	changes					1264:1270	liver pathological changes	1245:1270	liver pathological changes	1245:1270	LREE treatment significantly reduced NF-κB and LPS levels, improved liver pathological changes, and ameliorated intestinal ultrastructure injury.
31116255	11	12	theme	inflammatory	1662:1673	arg1	response					1675:1682	the inflammatory response	1658:1682	the inflammatory response	1658:1682	Thus, LREE could partly ameliorate microflora dysbiosis, suppress the inflammatory response, and attenuate liver injury in ALD rats.
31116255	1	13	theme	Linderae	216:223	arg1	extract					239:245	Linderae radix ethanol extract	216:245	Linderae radix ethanol extract (LREE)	216:252	This study aimed to explore the influence of gut microbiota alterations induced by Linderae radix ethanol extract (LREE) on alcoholic liver disease (ALD) in rats and to study the anti-inflammatory effect of LREE on ALD through the lipopolysaccharide (LPS) toll-like receptor 4 (TLR4)-nuclear factor kappa B (NF-κB) pathway.
31116255	1	13	theme	Linderae	216:223	arg1	LREE					248:251	LREE	248:251	LREE	248:251	This study aimed to explore the influence of gut microbiota alterations induced by Linderae radix ethanol extract (LREE) on alcoholic liver disease (ALD) in rats and to study the anti-inflammatory effect of LREE on ALD through the lipopolysaccharide (LPS) toll-like receptor 4 (TLR4)-nuclear factor kappa B (NF-κB) pathway.
31116255	12	14	theme	LPS-TLR4-NF-κB	1779:1792	arg1	pathway					1794:1800	the LPS-TLR4-NF-κB pathway	1775:1800	the LPS-TLR4-NF-κB pathway	1775:1800	The protective effect of LREE might be related to the LPS-TLR4-NF-κB pathway.
31116255	2	15	theme	%	515:515	arg1	administration					532:545	intragastric liquor [50% (v/v) ethanol] administration	492:545	intragastric liquor [50% (v/v) ethanol] administration at 10 mL/kg body weight for 20 days	492:581	ALD rat models were established by intragastric liquor [50% (v/v) ethanol] administration at 10 mL/kg body weight for 20 days.
31116255	2	15	theme	%	515:515	arg1	v/v					518:520	v/v	518:520	v/v	518:520	ALD rat models were established by intragastric liquor [50% (v/v) ethanol] administration at 10 mL/kg body weight for 20 days.
31116255	3	16	dep	group	683:687	arg1	rats					694:697	ALD rats	690:697	ALD rats fed with Essentiale	690:717	Rats were divided into six groups: normal group (no treatment), model group (ALD rats), Essentiale group (ALD rats fed with Essentiale, 137 mg/kg), and LREE high/moderate/low dose groups (ALD rats fed with 4, 2, or 1 g LREE/kg).
31116255	3	16	dep	group	683:687	arg1	mg/kg					724:728	137 mg/kg	720:728	137 mg/kg	720:728	Rats were divided into six groups: normal group (no treatment), model group (ALD rats), Essentiale group (ALD rats fed with Essentiale, 137 mg/kg), and LREE high/moderate/low dose groups (ALD rats fed with 4, 2, or 1 g LREE/kg).
31116255	9	17	theme	Meanwhile	1323:1331	arg1	groups					1343:1348	Meanwhile, LREE-fed groups	1323:1348	Meanwhile, LREE-fed groups	1323:1348	Meanwhile, LREE-fed groups showed a higher abundance of Firmicutes and a lower abundance of Bacteroidetes than the rats in the model group.
31116255	2	18	theme	liquor	505:510	arg1	administration					532:545	intragastric liquor [50% (v/v) ethanol] administration	492:545	intragastric liquor [50% (v/v) ethanol] administration at 10 mL/kg body weight for 20 days	492:581	ALD rat models were established by intragastric liquor [50% (v/v) ethanol] administration at 10 mL/kg body weight for 20 days.
31116255	2	18	theme	liquor	505:510	arg1	v/v					518:520	v/v	518:520	v/v	518:520	ALD rat models were established by intragastric liquor [50% (v/v) ethanol] administration at 10 mL/kg body weight for 20 days.
31116255	0	19	from	microbiota	113:122	arg1	rats					127:130	rats	127:130	rats	127:130	Linderae radix ethanol extract attenuates alcoholic liver injury via attenuating inflammation and regulating gut microbiota in rats.
31116255	9	20	from	rats	1438:1441	arg1	group					1456:1460	the model group	1446:1460	the model group	1446:1460	Meanwhile, LREE-fed groups showed a higher abundance of Firmicutes and a lower abundance of Bacteroidetes than the rats in the model group.
31116255	8	21	theme	ultrastructure	1300:1313	arg1	injury					1315:1320	intestinal ultrastructure injury	1289:1320	intestinal ultrastructure injury	1289:1320	LREE treatment significantly reduced NF-κB and LPS levels, improved liver pathological changes, and ameliorated intestinal ultrastructure injury.
31116255	7	22	theme	intestinal	1144:1153	arg1	tissue					1155:1160	intestinal tissue	1144:1160	intestinal tissue	1144:1160	Expression levels of TLR4 and CD68 in liver tissue, and occludin and claudin-1 in intestinal tissue were measured.
31116255	3	23	theme	ALD	661:663	arg1	group					654:658	model group	648:658	model group (ALD rats)	648:669	Rats were divided into six groups: normal group (no treatment), model group (ALD rats), Essentiale group (ALD rats fed with Essentiale, 137 mg/kg), and LREE high/moderate/low dose groups (ALD rats fed with 4, 2, or 1 g LREE/kg).
31116255	3	23	theme	ALD	661:663	arg1	rats					665:668	ALD rats	661:668	ALD rats	661:668	Rats were divided into six groups: normal group (no treatment), model group (ALD rats), Essentiale group (ALD rats fed with Essentiale, 137 mg/kg), and LREE high/moderate/low dose groups (ALD rats fed with 4, 2, or 1 g LREE/kg).
31116255	1	24	from	influence	165:173	arg1	ALD					282:284	ALD	282:284	ALD	282:284	This study aimed to explore the influence of gut microbiota alterations induced by Linderae radix ethanol extract (LREE) on alcoholic liver disease (ALD) in rats and to study the anti-inflammatory effect of LREE on ALD through the lipopolysaccharide (LPS) toll-like receptor 4 (TLR4)-nuclear factor kappa B (NF-κB) pathway.
31116255	1	24	from	influence	165:173	arg1	disease					273:279	alcoholic liver disease	257:279	alcoholic liver disease (ALD) in rats	257:293	This study aimed to explore the influence of gut microbiota alterations induced by Linderae radix ethanol extract (LREE) on alcoholic liver disease (ALD) in rats and to study the anti-inflammatory effect of LREE on ALD through the lipopolysaccharide (LPS) toll-like receptor 4 (TLR4)-nuclear factor kappa B (NF-κB) pathway.
31116255	9	25	theme	higher	1359:1364	arg1	abundance					1366:1374	a higher abundance	1357:1374	a higher abundance of Firmicutes	1357:1388	Meanwhile, LREE-fed groups showed a higher abundance of Firmicutes and a lower abundance of Bacteroidetes than the rats in the model group.
31116255	2	26	theme	rat	461:463	arg1	models					465:470	ALD rat models	457:470	ALD rat models	457:470	ALD rat models were established by intragastric liquor [50% (v/v) ethanol] administration at 10 mL/kg body weight for 20 days.
31116255	3	27	theme	Essentiale	672:681	arg1	group					683:687	Essentiale group	672:687	Essentiale group (ALD rats fed with Essentiale, 137 mg/kg)	672:729	Rats were divided into six groups: normal group (no treatment), model group (ALD rats), Essentiale group (ALD rats fed with Essentiale, 137 mg/kg), and LREE high/moderate/low dose groups (ALD rats fed with 4, 2, or 1 g LREE/kg).
31116255	3	27	theme	Essentiale	672:681	arg1	groups					611:616	six groups	607:616	six groups: normal group (no treatment), model group (ALD rats), Essentiale group (ALD rats fed with Essentiale, 137 mg/kg), and LREE high/moderate/low dose groups	607:769	Rats were divided into six groups: normal group (no treatment), model group (ALD rats), Essentiale group (ALD rats fed with Essentiale, 137 mg/kg), and LREE high/moderate/low dose groups (ALD rats fed with 4, 2, or 1 g LREE/kg).
31116255	5	28	theme	intestinal	881:890	arg1	ultrastructure					892:905	intestinal ultrastructure	881:905	intestinal ultrastructure	881:905	Liver pathological changes and intestinal ultrastructure were examined by hematoxylin and eosin staining and transmission electron microscopy.
31116255	3	29	theme	LREE	736:739	arg1	groups					764:769	LREE high/moderate/low dose groups	736:769	LREE high/moderate/low dose groups	736:769	Rats were divided into six groups: normal group (no treatment), model group (ALD rats), Essentiale group (ALD rats fed with Essentiale, 137 mg/kg), and LREE high/moderate/low dose groups (ALD rats fed with 4, 2, or 1 g LREE/kg).
31116255	2	30	theme	mL/kg	553:557	arg1	weight					564:569	10 mL/kg body weight	550:569	10 mL/kg body weight for 20 days	550:581	ALD rat models were established by intragastric liquor [50% (v/v) ethanol] administration at 10 mL/kg body weight for 20 days.
31116255	10	31	theme	LREE	1481:1484	arg1	Administration					1463:1476	Administration	1463:1476	Administration of LREE	1463:1484	Administration of LREE suppressed TLR4 overexpression and promoted the expression of occludin and claudin-1 in intestine tissue.
31116255	1	32	theme	LREE	340:343	arg1	effect					330:335	the anti-inflammatory effect	308:335	the anti-inflammatory effect of LREE on ALD through the lipopolysaccharide (LPS) toll-like receptor 4 (TLR4)-nuclear factor kappa B (NF-κB) pathway	308:454	This study aimed to explore the influence of gut microbiota alterations induced by Linderae radix ethanol extract (LREE) on alcoholic liver disease (ALD) in rats and to study the anti-inflammatory effect of LREE on ALD through the lipopolysaccharide (LPS) toll-like receptor 4 (TLR4)-nuclear factor kappa B (NF-κB) pathway.
31116255	4	33	theme	NF-κB	813:817	arg1	levels					827:832	NF-κB and LPS levels	813:832	NF-κB and LPS levels	813:832	NF-κB and LPS levels were evaluated.
31116255	8	34	theme	LREE	1177:1180	arg1	treatment					1182:1190	LREE treatment	1177:1190	LREE treatment	1177:1190	LREE treatment significantly reduced NF-κB and LPS levels, improved liver pathological changes, and ameliorated intestinal ultrastructure injury.
31116255	0	35	theme	Linderae	0:7	arg1	extract					23:29	Linderae radix ethanol extract	0:29	Linderae radix ethanol extract	0:29	Linderae radix ethanol extract attenuates alcoholic liver injury via attenuating inflammation and regulating gut microbiota in rats.
31116255	1	36	theme	liver	267:271	arg1	ALD					282:284	ALD	282:284	ALD	282:284	This study aimed to explore the influence of gut microbiota alterations induced by Linderae radix ethanol extract (LREE) on alcoholic liver disease (ALD) in rats and to study the anti-inflammatory effect of LREE on ALD through the lipopolysaccharide (LPS) toll-like receptor 4 (TLR4)-nuclear factor kappa B (NF-κB) pathway.
31116255	1	36	theme	liver	267:271	arg1	disease					273:279	alcoholic liver disease	257:279	alcoholic liver disease (ALD) in rats	257:293	This study aimed to explore the influence of gut microbiota alterations induced by Linderae radix ethanol extract (LREE) on alcoholic liver disease (ALD) in rats and to study the anti-inflammatory effect of LREE on ALD through the lipopolysaccharide (LPS) toll-like receptor 4 (TLR4)-nuclear factor kappa B (NF-κB) pathway.
31116255	12	37	theme	protective	1729:1738	arg1	related					1764:1770	related	1764:1770	related	1764:1770	The protective effect of LREE might be related to the LPS-TLR4-NF-κB pathway.
31116255	12	37	theme	protective	1729:1738	arg1	effect					1740:1745	The protective effect	1725:1745	The protective effect of LREE	1725:1753	The protective effect of LREE might be related to the LPS-TLR4-NF-κB pathway.
31116255	9	38	theme	lower	1396:1400	arg1	abundance					1402:1410	a lower abundance	1394:1410	a lower abundance of Bacteroidetes	1394:1427	Meanwhile, LREE-fed groups showed a higher abundance of Firmicutes and a lower abundance of Bacteroidetes than the rats in the model group.
31116255	4	39	theme	LPS	823:825	arg1	levels					827:832	NF-κB and LPS levels	813:832	NF-κB and LPS levels	813:832	NF-κB and LPS levels were evaluated.
31116255	10	40	from	expression	1534:1543	arg1	tissue					1584:1589	intestine tissue	1574:1589	intestine tissue	1574:1589	Administration of LREE suppressed TLR4 overexpression and promoted the expression of occludin and claudin-1 in intestine tissue.
31116255	0	41	theme	ethanol	15:21	arg1	extract					23:29	Linderae radix ethanol extract	0:29	Linderae radix ethanol extract	0:29	Linderae radix ethanol extract attenuates alcoholic liver injury via attenuating inflammation and regulating gut microbiota in rats.
31116255	3	42	theme	dose	759:762	arg1	groups					764:769	LREE high/moderate/low dose groups	736:769	LREE high/moderate/low dose groups	736:769	Rats were divided into six groups: normal group (no treatment), model group (ALD rats), Essentiale group (ALD rats fed with Essentiale, 137 mg/kg), and LREE high/moderate/low dose groups (ALD rats fed with 4, 2, or 1 g LREE/kg).
31116255	8	43	theme	NF-κB	1214:1218	arg1	levels					1228:1233	NF-κB and LPS levels	1214:1233	NF-κB and LPS levels	1214:1233	LREE treatment significantly reduced NF-κB and LPS levels, improved liver pathological changes, and ameliorated intestinal ultrastructure injury.
31116255	10	44	theme	TLR4	1497:1500	arg1	overexpression					1502:1515	TLR4 overexpression	1497:1515	TLR4 overexpression	1497:1515	Administration of LREE suppressed TLR4 overexpression and promoted the expression of occludin and claudin-1 in intestine tissue.
31116255	3	45	dep	groups	611:616	arg1	groups					764:769	LREE high/moderate/low dose groups	736:769	LREE high/moderate/low dose groups	736:769	Rats were divided into six groups: normal group (no treatment), model group (ALD rats), Essentiale group (ALD rats fed with Essentiale, 137 mg/kg), and LREE high/moderate/low dose groups (ALD rats fed with 4, 2, or 1 g LREE/kg).
31116255	3	45	dep	groups	611:616	arg1	group					626:630	normal group	619:630	normal group (no treatment)	619:645	Rats were divided into six groups: normal group (no treatment), model group (ALD rats), Essentiale group (ALD rats fed with Essentiale, 137 mg/kg), and LREE high/moderate/low dose groups (ALD rats fed with 4, 2, or 1 g LREE/kg).
31116255	3	45	dep	groups	611:616	arg1	group					654:658	model group	648:658	model group (ALD rats)	648:669	Rats were divided into six groups: normal group (no treatment), model group (ALD rats), Essentiale group (ALD rats fed with Essentiale, 137 mg/kg), and LREE high/moderate/low dose groups (ALD rats fed with 4, 2, or 1 g LREE/kg).
31116255	3	45	dep	groups	611:616	arg1	group					683:687	Essentiale group	672:687	Essentiale group (ALD rats fed with Essentiale, 137 mg/kg)	672:729	Rats were divided into six groups: normal group (no treatment), model group (ALD rats), Essentiale group (ALD rats fed with Essentiale, 137 mg/kg), and LREE high/moderate/low dose groups (ALD rats fed with 4, 2, or 1 g LREE/kg).
31116255	3	45	dep	groups	611:616	arg1	treatment					636:644	no treatment	633:644	no treatment	633:644	Rats were divided into six groups: normal group (no treatment), model group (ALD rats), Essentiale group (ALD rats fed with Essentiale, 137 mg/kg), and LREE high/moderate/low dose groups (ALD rats fed with 4, 2, or 1 g LREE/kg).
31116255	3	45	dep	groups	611:616	arg1	rats					665:668	ALD rats	661:668	ALD rats	661:668	Rats were divided into six groups: normal group (no treatment), model group (ALD rats), Essentiale group (ALD rats fed with Essentiale, 137 mg/kg), and LREE high/moderate/low dose groups (ALD rats fed with 4, 2, or 1 g LREE/kg).
31116255	3	45	dep	groups	611:616	arg1	groups					611:616	six groups	607:616	six groups: normal group (no treatment), model group (ALD rats), Essentiale group (ALD rats fed with Essentiale, 137 mg/kg), and LREE high/moderate/low dose groups	607:769	Rats were divided into six groups: normal group (no treatment), model group (ALD rats), Essentiale group (ALD rats fed with Essentiale, 137 mg/kg), and LREE high/moderate/low dose groups (ALD rats fed with 4, 2, or 1 g LREE/kg).
31116255	8	46	theme	LPS	1224:1226	arg1	levels					1228:1233	NF-κB and LPS levels	1214:1233	NF-κB and LPS levels	1214:1233	LREE treatment significantly reduced NF-κB and LPS levels, improved liver pathological changes, and ameliorated intestinal ultrastructure injury.
31116255	0	47	theme	liver	52:56	arg1	injury					58:63	alcoholic liver injury	42:63	alcoholic liver injury	42:63	Linderae radix ethanol extract attenuates alcoholic liver injury via attenuating inflammation and regulating gut microbiota in rats.
31116255	5	48	theme	transmission	959:970	arg1	microscopy					981:990	transmission electron microscopy	959:990	transmission electron microscopy	959:990	Liver pathological changes and intestinal ultrastructure were examined by hematoxylin and eosin staining and transmission electron microscopy.
31116255	7	49	from	occludin	1118:1125	arg1	tissue					1155:1160	intestinal tissue	1144:1160	intestinal tissue	1144:1160	Expression levels of TLR4 and CD68 in liver tissue, and occludin and claudin-1 in intestinal tissue were measured.
31116255	7	49	from	occludin	1118:1125	arg1	tissue					1106:1111	liver tissue	1100:1111	liver tissue	1100:1111	Expression levels of TLR4 and CD68 in liver tissue, and occludin and claudin-1 in intestinal tissue were measured.
31116255	8	50	theme	liver	1245:1249	arg1	changes					1264:1270	liver pathological changes	1245:1270	liver pathological changes	1245:1270	LREE treatment significantly reduced NF-κB and LPS levels, improved liver pathological changes, and ameliorated intestinal ultrastructure injury.
31116255	11	51	theme	ALD	1715:1717	arg1	rats					1719:1722	ALD rats	1715:1722	ALD rats	1715:1722	Thus, LREE could partly ameliorate microflora dysbiosis, suppress the inflammatory response, and attenuate liver injury in ALD rats.
31116255	7	52	from	claudin-1	1131:1139	arg1	tissue					1155:1160	intestinal tissue	1144:1160	intestinal tissue	1144:1160	Expression levels of TLR4 and CD68 in liver tissue, and occludin and claudin-1 in intestinal tissue were measured.
31116255	7	52	from	claudin-1	1131:1139	arg1	tissue					1106:1111	liver tissue	1100:1111	liver tissue	1100:1111	Expression levels of TLR4 and CD68 in liver tissue, and occludin and claudin-1 in intestinal tissue were measured.
31116255	7	53	theme	Expression	1062:1071	arg1	levels					1073:1078	Expression levels	1062:1078	Expression levels of TLR4 and CD68 in liver tissue	1062:1111	Expression levels of TLR4 and CD68 in liver tissue, and occludin and claudin-1 in intestinal tissue were measured.
31116255	7	54	from	levels	1073:1078	arg1	tissue					1155:1160	intestinal tissue	1144:1160	intestinal tissue	1144:1160	Expression levels of TLR4 and CD68 in liver tissue, and occludin and claudin-1 in intestinal tissue were measured.
31116255	7	54	from	levels	1073:1078	arg1	tissue					1106:1111	liver tissue	1100:1111	liver tissue	1100:1111	Expression levels of TLR4 and CD68 in liver tissue, and occludin and claudin-1 in intestinal tissue were measured.
31116255	7	55	from	TLR4	1083:1086	arg1	tissue					1106:1111	liver tissue	1100:1111	liver tissue	1100:1111	Expression levels of TLR4 and CD68 in liver tissue, and occludin and claudin-1 in intestinal tissue were measured.
31116255	1	56	theme	-nuclear	416:423	arg1	NF-κB					441:445	NF-κB	441:445	NF-κB	441:445	This study aimed to explore the influence of gut microbiota alterations induced by Linderae radix ethanol extract (LREE) on alcoholic liver disease (ALD) in rats and to study the anti-inflammatory effect of LREE on ALD through the lipopolysaccharide (LPS) toll-like receptor 4 (TLR4)-nuclear factor kappa B (NF-κB) pathway.
31116255	1	56	theme	-nuclear	416:423	arg1	B					438:438	lipopolysaccharide (LPS) toll-like receptor 4 (TLR4)-nuclear factor kappa B	364:438	the lipopolysaccharide (LPS) toll-like receptor 4 (TLR4)-nuclear factor kappa B (NF-κB) pathway	360:454	This study aimed to explore the influence of gut microbiota alterations induced by Linderae radix ethanol extract (LREE) on alcoholic liver disease (ALD) in rats and to study the anti-inflammatory effect of LREE on ALD through the lipopolysaccharide (LPS) toll-like receptor 4 (TLR4)-nuclear factor kappa B (NF-κB) pathway.
31116255	1	57	theme	gut	178:180	arg1	alterations					193:203	gut microbiota alterations	178:203	gut microbiota alterations induced by Linderae radix ethanol extract (LREE)	178:252	This study aimed to explore the influence of gut microbiota alterations induced by Linderae radix ethanol extract (LREE) on alcoholic liver disease (ALD) in rats and to study the anti-inflammatory effect of LREE on ALD through the lipopolysaccharide (LPS) toll-like receptor 4 (TLR4)-nuclear factor kappa B (NF-κB) pathway.
31116255	7	58	from	CD68	1092:1095	arg1	tissue					1106:1111	liver tissue	1100:1111	liver tissue	1100:1111	Expression levels of TLR4 and CD68 in liver tissue, and occludin and claudin-1 in intestinal tissue were measured.
31116255	1	59	theme	kappa	432:436	arg1	NF-κB					441:445	NF-κB	441:445	NF-κB	441:445	This study aimed to explore the influence of gut microbiota alterations induced by Linderae radix ethanol extract (LREE) on alcoholic liver disease (ALD) in rats and to study the anti-inflammatory effect of LREE on ALD through the lipopolysaccharide (LPS) toll-like receptor 4 (TLR4)-nuclear factor kappa B (NF-κB) pathway.
31116255	1	59	theme	kappa	432:436	arg1	B					438:438	lipopolysaccharide (LPS) toll-like receptor 4 (TLR4)-nuclear factor kappa B	364:438	the lipopolysaccharide (LPS) toll-like receptor 4 (TLR4)-nuclear factor kappa B (NF-κB) pathway	360:454	This study aimed to explore the influence of gut microbiota alterations induced by Linderae radix ethanol extract (LREE) on alcoholic liver disease (ALD) in rats and to study the anti-inflammatory effect of LREE on ALD through the lipopolysaccharide (LPS) toll-like receptor 4 (TLR4)-nuclear factor kappa B (NF-κB) pathway.
31116255	1	60	theme	alterations	193:203	arg1	influence					165:173	the influence	161:173	the influence of gut microbiota alterations induced by Linderae radix ethanol extract (LREE) on alcoholic liver disease (ALD) in rats	161:293	This study aimed to explore the influence of gut microbiota alterations induced by Linderae radix ethanol extract (LREE) on alcoholic liver disease (ALD) in rats and to study the anti-inflammatory effect of LREE on ALD through the lipopolysaccharide (LPS) toll-like receptor 4 (TLR4)-nuclear factor kappa B (NF-κB) pathway.
31116255	3	61	theme	no	633:634	arg1	treatment					636:644	no treatment	633:644	no treatment	633:644	Rats were divided into six groups: normal group (no treatment), model group (ALD rats), Essentiale group (ALD rats fed with Essentiale, 137 mg/kg), and LREE high/moderate/low dose groups (ALD rats fed with 4, 2, or 1 g LREE/kg).
31116255	3	61	theme	no	633:634	arg1	group					626:630	normal group	619:630	normal group (no treatment)	619:645	Rats were divided into six groups: normal group (no treatment), model group (ALD rats), Essentiale group (ALD rats fed with Essentiale, 137 mg/kg), and LREE high/moderate/low dose groups (ALD rats fed with 4, 2, or 1 g LREE/kg).
31116255	5	62	theme	pathological	856:867	arg1	changes					869:875	Liver pathological changes	850:875	Liver pathological changes	850:875	Liver pathological changes and intestinal ultrastructure were examined by hematoxylin and eosin staining and transmission electron microscopy.
31116255	6	63	theme	rDNA	1045:1048	arg1	sequencing					1050:1059	rDNA sequencing	1045:1059	rDNA sequencing	1045:1059	The gut microbiota composition was evaluated by 16S rDNA sequencing.
31116255	1	64	theme	lipopolysaccharide	364:381	arg1	receptor					399:406	lipopolysaccharide (LPS) toll-like receptor 4	364:408	lipopolysaccharide (LPS) toll-like receptor 4 (TLR4)	364:415	This study aimed to explore the influence of gut microbiota alterations induced by Linderae radix ethanol extract (LREE) on alcoholic liver disease (ALD) in rats and to study the anti-inflammatory effect of LREE on ALD through the lipopolysaccharide (LPS) toll-like receptor 4 (TLR4)-nuclear factor kappa B (NF-κB) pathway.
31116255	1	64	theme	lipopolysaccharide	364:381	arg1	TLR4					411:414	TLR4	411:414	TLR4	411:414	This study aimed to explore the influence of gut microbiota alterations induced by Linderae radix ethanol extract (LREE) on alcoholic liver disease (ALD) in rats and to study the anti-inflammatory effect of LREE on ALD through the lipopolysaccharide (LPS) toll-like receptor 4 (TLR4)-nuclear factor kappa B (NF-κB) pathway.
31116255	5	65	theme	eosin	940:944	arg1	staining					946:953	hematoxylin and eosin staining	924:953	hematoxylin and eosin staining	924:953	Liver pathological changes and intestinal ultrastructure were examined by hematoxylin and eosin staining and transmission electron microscopy.
31116255	2	66	theme	[50	512:514	arg1	administration					532:545	intragastric liquor [50% (v/v) ethanol] administration	492:545	intragastric liquor [50% (v/v) ethanol] administration at 10 mL/kg body weight for 20 days	492:581	ALD rat models were established by intragastric liquor [50% (v/v) ethanol] administration at 10 mL/kg body weight for 20 days.
31116255	2	66	theme	[50	512:514	arg1	v/v					518:520	v/v	518:520	v/v	518:520	ALD rat models were established by intragastric liquor [50% (v/v) ethanol] administration at 10 mL/kg body weight for 20 days.
31116255	8	67	theme	intestinal	1289:1298	arg1	injury					1315:1320	intestinal ultrastructure injury	1289:1320	intestinal ultrastructure injury	1289:1320	LREE treatment significantly reduced NF-κB and LPS levels, improved liver pathological changes, and ameliorated intestinal ultrastructure injury.
31116255	3	68	theme	model	648:652	arg1	group					654:658	model group	648:658	model group (ALD rats)	648:669	Rats were divided into six groups: normal group (no treatment), model group (ALD rats), Essentiale group (ALD rats fed with Essentiale, 137 mg/kg), and LREE high/moderate/low dose groups (ALD rats fed with 4, 2, or 1 g LREE/kg).
31116255	3	68	theme	model	648:652	arg1	rats					665:668	ALD rats	661:668	ALD rats	661:668	Rats were divided into six groups: normal group (no treatment), model group (ALD rats), Essentiale group (ALD rats fed with Essentiale, 137 mg/kg), and LREE high/moderate/low dose groups (ALD rats fed with 4, 2, or 1 g LREE/kg).
31116255	3	68	theme	model	648:652	arg1	groups					611:616	six groups	607:616	six groups: normal group (no treatment), model group (ALD rats), Essentiale group (ALD rats fed with Essentiale, 137 mg/kg), and LREE high/moderate/low dose groups	607:769	Rats were divided into six groups: normal group (no treatment), model group (ALD rats), Essentiale group (ALD rats fed with Essentiale, 137 mg/kg), and LREE high/moderate/low dose groups (ALD rats fed with 4, 2, or 1 g LREE/kg).
31116255	1	69	theme	LPS	384:386	arg1	receptor					399:406	lipopolysaccharide (LPS) toll-like receptor 4	364:408	lipopolysaccharide (LPS) toll-like receptor 4 (TLR4)	364:415	This study aimed to explore the influence of gut microbiota alterations induced by Linderae radix ethanol extract (LREE) on alcoholic liver disease (ALD) in rats and to study the anti-inflammatory effect of LREE on ALD through the lipopolysaccharide (LPS) toll-like receptor 4 (TLR4)-nuclear factor kappa B (NF-κB) pathway.
31116255	1	69	theme	LPS	384:386	arg1	TLR4					411:414	TLR4	411:414	TLR4	411:414	This study aimed to explore the influence of gut microbiota alterations induced by Linderae radix ethanol extract (LREE) on alcoholic liver disease (ALD) in rats and to study the anti-inflammatory effect of LREE on ALD through the lipopolysaccharide (LPS) toll-like receptor 4 (TLR4)-nuclear factor kappa B (NF-κB) pathway.
31116255	2	70	theme	intragastric	492:503	arg1	administration					532:545	intragastric liquor [50% (v/v) ethanol] administration	492:545	intragastric liquor [50% (v/v) ethanol] administration at 10 mL/kg body weight for 20 days	492:581	ALD rat models were established by intragastric liquor [50% (v/v) ethanol] administration at 10 mL/kg body weight for 20 days.
31116255	2	70	theme	intragastric	492:503	arg1	v/v					518:520	v/v	518:520	v/v	518:520	ALD rat models were established by intragastric liquor [50% (v/v) ethanol] administration at 10 mL/kg body weight for 20 days.
31116255	2	71	from	weight	564:569	arg1	administration					532:545	intragastric liquor [50% (v/v) ethanol] administration	492:545	intragastric liquor [50% (v/v) ethanol] administration at 10 mL/kg body weight for 20 days	492:581	ALD rat models were established by intragastric liquor [50% (v/v) ethanol] administration at 10 mL/kg body weight for 20 days.
31116255	2	71	from	weight	564:569	arg1	v/v					518:520	v/v	518:520	v/v	518:520	ALD rat models were established by intragastric liquor [50% (v/v) ethanol] administration at 10 mL/kg body weight for 20 days.
31116255	1	72	theme	toll-like	389:397	arg1	receptor					399:406	lipopolysaccharide (LPS) toll-like receptor 4	364:408	lipopolysaccharide (LPS) toll-like receptor 4 (TLR4)	364:415	This study aimed to explore the influence of gut microbiota alterations induced by Linderae radix ethanol extract (LREE) on alcoholic liver disease (ALD) in rats and to study the anti-inflammatory effect of LREE on ALD through the lipopolysaccharide (LPS) toll-like receptor 4 (TLR4)-nuclear factor kappa B (NF-κB) pathway.
31116255	1	72	theme	toll-like	389:397	arg1	TLR4					411:414	TLR4	411:414	TLR4	411:414	This study aimed to explore the influence of gut microbiota alterations induced by Linderae radix ethanol extract (LREE) on alcoholic liver disease (ALD) in rats and to study the anti-inflammatory effect of LREE on ALD through the lipopolysaccharide (LPS) toll-like receptor 4 (TLR4)-nuclear factor kappa B (NF-κB) pathway.
31116255	6	73	theme	microbiota	1001:1010	arg1	composition					1012:1022	The gut microbiota composition	993:1022	The gut microbiota composition	993:1022	The gut microbiota composition was evaluated by 16S rDNA sequencing.
31116255	5	74	theme	hematoxylin	924:934	arg1	staining					946:953	hematoxylin and eosin staining	924:953	hematoxylin and eosin staining	924:953	Liver pathological changes and intestinal ultrastructure were examined by hematoxylin and eosin staining and transmission electron microscopy.
31116255	12	75	theme	LREE	1750:1753	arg1	related					1764:1770	related	1764:1770	related	1764:1770	The protective effect of LREE might be related to the LPS-TLR4-NF-κB pathway.
31116255	12	75	theme	LREE	1750:1753	arg1	effect					1740:1745	The protective effect	1725:1745	The protective effect of LREE	1725:1753	The protective effect of LREE might be related to the LPS-TLR4-NF-κB pathway.
31116255	11	76	from	injury	1705:1710	arg1	rats					1719:1722	ALD rats	1715:1722	ALD rats	1715:1722	Thus, LREE could partly ameliorate microflora dysbiosis, suppress the inflammatory response, and attenuate liver injury in ALD rats.
31116255	3	77	theme	ALD	690:692	arg1	rats					694:697	ALD rats	690:697	ALD rats fed with Essentiale	690:717	Rats were divided into six groups: normal group (no treatment), model group (ALD rats), Essentiale group (ALD rats fed with Essentiale, 137 mg/kg), and LREE high/moderate/low dose groups (ALD rats fed with 4, 2, or 1 g LREE/kg).
31116255	3	77	theme	ALD	690:692	arg1	mg/kg					724:728	137 mg/kg	720:728	137 mg/kg	720:728	Rats were divided into six groups: normal group (no treatment), model group (ALD rats), Essentiale group (ALD rats fed with Essentiale, 137 mg/kg), and LREE high/moderate/low dose groups (ALD rats fed with 4, 2, or 1 g LREE/kg).
31116255	0	78	theme	gut	109:111	arg1	microbiota					113:122	gut microbiota	109:122	gut microbiota in rats	109:130	Linderae radix ethanol extract attenuates alcoholic liver injury via attenuating inflammation and regulating gut microbiota in rats.
31116255	1	79	theme	anti-inflammatory	312:328	arg1	effect					330:335	the anti-inflammatory effect	308:335	the anti-inflammatory effect of LREE on ALD through the lipopolysaccharide (LPS) toll-like receptor 4 (TLR4)-nuclear factor kappa B (NF-κB) pathway	308:454	This study aimed to explore the influence of gut microbiota alterations induced by Linderae radix ethanol extract (LREE) on alcoholic liver disease (ALD) in rats and to study the anti-inflammatory effect of LREE on ALD through the lipopolysaccharide (LPS) toll-like receptor 4 (TLR4)-nuclear factor kappa B (NF-κB) pathway.
31116255	2	80	theme	ALD	457:459	arg1	models					465:470	ALD rat models	457:470	ALD rat models	457:470	ALD rat models were established by intragastric liquor [50% (v/v) ethanol] administration at 10 mL/kg body weight for 20 days.
31116255	2	81	theme	body	559:562	arg1	weight					564:569	10 mL/kg body weight	550:569	10 mL/kg body weight for 20 days	550:581	ALD rat models were established by intragastric liquor [50% (v/v) ethanol] administration at 10 mL/kg body weight for 20 days.
31116255	9	82	theme	Firmicutes	1379:1388	arg1	abundance					1366:1374	a higher abundance	1357:1374	a higher abundance of Firmicutes	1357:1388	Meanwhile, LREE-fed groups showed a higher abundance of Firmicutes and a lower abundance of Bacteroidetes than the rats in the model group.
31116255	9	82	theme	Firmicutes	1379:1388	arg1	abundance					1402:1410	a lower abundance	1394:1410	a lower abundance of Bacteroidetes	1394:1427	Meanwhile, LREE-fed groups showed a higher abundance of Firmicutes and a lower abundance of Bacteroidetes than the rats in the model group.
31116255	3	83	theme	high/moderate/low	741:757	arg1	groups					764:769	LREE high/moderate/low dose groups	736:769	LREE high/moderate/low dose groups	736:769	Rats were divided into six groups: normal group (no treatment), model group (ALD rats), Essentiale group (ALD rats fed with Essentiale, 137 mg/kg), and LREE high/moderate/low dose groups (ALD rats fed with 4, 2, or 1 g LREE/kg).
31116255	9	84	dep	Meanwhile	1323:1331	arg1	LREE-fed					1334:1341	LREE-fed	1334:1341	LREE-fed	1334:1341	Meanwhile, LREE-fed groups showed a higher abundance of Firmicutes and a lower abundance of Bacteroidetes than the rats in the model group.
31116255	0	85	theme	radix	9:13	arg1	extract					23:29	Linderae radix ethanol extract	0:29	Linderae radix ethanol extract	0:29	Linderae radix ethanol extract attenuates alcoholic liver injury via attenuating inflammation and regulating gut microbiota in rats.
31116255	11	86	theme	microflora	1627:1636	arg1	dysbiosis					1638:1646	microflora dysbiosis	1627:1646	microflora dysbiosis	1627:1646	Thus, LREE could partly ameliorate microflora dysbiosis, suppress the inflammatory response, and attenuate liver injury in ALD rats.
31116255	1	87	from	effect	330:335	arg1	ALD					348:350	ALD	348:350	ALD through the lipopolysaccharide (LPS) toll-like receptor 4 (TLR4)-nuclear factor kappa B (NF-κB) pathway	348:454	This study aimed to explore the influence of gut microbiota alterations induced by Linderae radix ethanol extract (LREE) on alcoholic liver disease (ALD) in rats and to study the anti-inflammatory effect of LREE on ALD through the lipopolysaccharide (LPS) toll-like receptor 4 (TLR4)-nuclear factor kappa B (NF-κB) pathway.
31116255	3	88	dep	divided	594:600	arg1	rats					776:779	ALD rats	772:779	ALD rats fed with 4, 2, or 1 g LREE/kg	772:809	Rats were divided into six groups: normal group (no treatment), model group (ALD rats), Essentiale group (ALD rats fed with Essentiale, 137 mg/kg), and LREE high/moderate/low dose groups (ALD rats fed with 4, 2, or 1 g LREE/kg).
31116255	3	89	theme	ALD	772:774	arg1	rats					776:779	ALD rats	772:779	ALD rats fed with 4, 2, or 1 g LREE/kg	772:809	Rats were divided into six groups: normal group (no treatment), model group (ALD rats), Essentiale group (ALD rats fed with Essentiale, 137 mg/kg), and LREE high/moderate/low dose groups (ALD rats fed with 4, 2, or 1 g LREE/kg).
31116255	0	90	theme	alcoholic	42:50	arg1	injury					58:63	alcoholic liver injury	42:63	alcoholic liver injury	42:63	Linderae radix ethanol extract attenuates alcoholic liver injury via attenuating inflammation and regulating gut microbiota in rats.
31116255	9	91	theme	Bacteroidetes	1415:1427	arg1	abundance					1366:1374	a higher abundance	1357:1374	a higher abundance of Firmicutes	1357:1388	Meanwhile, LREE-fed groups showed a higher abundance of Firmicutes and a lower abundance of Bacteroidetes than the rats in the model group.
31116255	9	91	theme	Bacteroidetes	1415:1427	arg1	abundance					1402:1410	a lower abundance	1394:1410	a lower abundance of Bacteroidetes	1394:1427	Meanwhile, LREE-fed groups showed a higher abundance of Firmicutes and a lower abundance of Bacteroidetes than the rats in the model group.
31116255	7	92	theme	liver	1100:1104	arg1	tissue					1106:1111	liver tissue	1100:1111	liver tissue	1100:1111	Expression levels of TLR4 and CD68 in liver tissue, and occludin and claudin-1 in intestinal tissue were measured.
31116255	1	93	theme	B	438:438	arg1	pathway					448:454	the lipopolysaccharide (LPS) toll-like receptor 4 (TLR4)-nuclear factor kappa B (NF-κB) pathway	360:454	the lipopolysaccharide (LPS) toll-like receptor 4 (TLR4)-nuclear factor kappa B (NF-κB) pathway	360:454	This study aimed to explore the influence of gut microbiota alterations induced by Linderae radix ethanol extract (LREE) on alcoholic liver disease (ALD) in rats and to study the anti-inflammatory effect of LREE on ALD through the lipopolysaccharide (LPS) toll-like receptor 4 (TLR4)-nuclear factor kappa B (NF-κB) pathway.
31116255	7	94	from	tissue	1106:1111	arg1	levels					1073:1078	Expression levels	1062:1078	Expression levels of TLR4 and CD68 in liver tissue	1062:1111	Expression levels of TLR4 and CD68 in liver tissue, and occludin and claudin-1 in intestinal tissue were measured.
31116255	7	94	from	tissue	1106:1111	arg1	claudin-1					1131:1139	claudin-1	1131:1139	claudin-1	1131:1139	Expression levels of TLR4 and CD68 in liver tissue, and occludin and claudin-1 in intestinal tissue were measured.
31116255	7	94	from	tissue	1106:1111	arg1	occludin					1118:1125	occludin	1118:1125	occludin	1118:1125	Expression levels of TLR4 and CD68 in liver tissue, and occludin and claudin-1 in intestinal tissue were measured.
31116255	7	95	theme	CD68	1092:1095	arg1	levels					1073:1078	Expression levels	1062:1078	Expression levels of TLR4 and CD68 in liver tissue	1062:1111	Expression levels of TLR4 and CD68 in liver tissue, and occludin and claudin-1 in intestinal tissue were measured.
31116255	7	95	theme	CD68	1092:1095	arg1	claudin-1					1131:1139	claudin-1	1131:1139	claudin-1	1131:1139	Expression levels of TLR4 and CD68 in liver tissue, and occludin and claudin-1 in intestinal tissue were measured.
31116255	7	95	theme	CD68	1092:1095	arg1	occludin					1118:1125	occludin	1118:1125	occludin	1118:1125	Expression levels of TLR4 and CD68 in liver tissue, and occludin and claudin-1 in intestinal tissue were measured.
31116255	11	96	theme	liver	1699:1703	arg1	injury					1705:1710	liver injury	1699:1710	liver injury in ALD rats	1699:1722	Thus, LREE could partly ameliorate microflora dysbiosis, suppress the inflammatory response, and attenuate liver injury in ALD rats.
31116255	5	97	theme	electron	972:979	arg1	microscopy					981:990	transmission electron microscopy	959:990	transmission electron microscopy	959:990	Liver pathological changes and intestinal ultrastructure were examined by hematoxylin and eosin staining and transmission electron microscopy.
31116255	7	98	theme	TLR4	1083:1086	arg1	levels					1073:1078	Expression levels	1062:1078	Expression levels of TLR4 and CD68 in liver tissue	1062:1111	Expression levels of TLR4 and CD68 in liver tissue, and occludin and claudin-1 in intestinal tissue were measured.
31116255	7	98	theme	TLR4	1083:1086	arg1	claudin-1					1131:1139	claudin-1	1131:1139	claudin-1	1131:1139	Expression levels of TLR4 and CD68 in liver tissue, and occludin and claudin-1 in intestinal tissue were measured.
31116255	7	98	theme	TLR4	1083:1086	arg1	occludin					1118:1125	occludin	1118:1125	occludin	1118:1125	Expression levels of TLR4 and CD68 in liver tissue, and occludin and claudin-1 in intestinal tissue were measured.
31116255	1	99	dep	Linderae	216:223	arg1	radix					225:229	radix	225:229	radix	225:229	This study aimed to explore the influence of gut microbiota alterations induced by Linderae radix ethanol extract (LREE) on alcoholic liver disease (ALD) in rats and to study the anti-inflammatory effect of LREE on ALD through the lipopolysaccharide (LPS) toll-like receptor 4 (TLR4)-nuclear factor kappa B (NF-κB) pathway.
31116255	1	100	theme	ethanol	231:237	arg1	extract					239:245	Linderae radix ethanol extract	216:245	Linderae radix ethanol extract (LREE)	216:252	This study aimed to explore the influence of gut microbiota alterations induced by Linderae radix ethanol extract (LREE) on alcoholic liver disease (ALD) in rats and to study the anti-inflammatory effect of LREE on ALD through the lipopolysaccharide (LPS) toll-like receptor 4 (TLR4)-nuclear factor kappa B (NF-κB) pathway.
31116255	1	100	theme	ethanol	231:237	arg1	LREE					248:251	LREE	248:251	LREE	248:251	This study aimed to explore the influence of gut microbiota alterations induced by Linderae radix ethanol extract (LREE) on alcoholic liver disease (ALD) in rats and to study the anti-inflammatory effect of LREE on ALD through the lipopolysaccharide (LPS) toll-like receptor 4 (TLR4)-nuclear factor kappa B (NF-κB) pathway.
31116255	9	101	theme	model	1450:1454	arg1	group					1456:1460	the model group	1446:1460	the model group	1446:1460	Meanwhile, LREE-fed groups showed a higher abundance of Firmicutes and a lower abundance of Bacteroidetes than the rats in the model group.
31116255	10	102	theme	claudin-1	1561:1569	arg1	expression					1534:1543	the expression	1530:1543	the expression of occludin and claudin-1 in intestine tissue	1530:1589	Administration of LREE suppressed TLR4 overexpression and promoted the expression of occludin and claudin-1 in intestine tissue.
31155242	10	0	theme	LMP	1813:1815	arg1	level					1817:1821	the LMP level	1809:1821	the LMP level	1809:1821	Compared with the LMP level, the HMP level increased arterial concentrations of BHB, urea, and all EAA groups and decreased the concentration of total non-EAA.
31155242	2	1	theme	palm	760:763	arg1	olein					765:769	676 g/d palm olein	752:769	676 g/d palm olein	752:769	According to a 6 × 6 Latin square design, 6 rumen-fistulated second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were abomasally infused with saline (LMP-C); isoenergetic infusions (digestible energy basis) of 1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA (EAA; HMP-C); or isoenergetic infusions of 1,319 g/d glucose + 844 g/d EAA (HMP-GG) or 676 g/d palm olein + 844 g/d EAA (HMP-LG).
31155242	15	2	theme	GG	2428:2429	arg1	Infusion					2416:2423	Infusion	2416:2423	Infusion of GG	2416:2429	Infusion of GG tended to increase mammary glucose uptake, and tended to decrease BHB uptake only at the LMP level.
31155242	12	3	theme	AA	2134:2135	arg1	interactions					2137:2148	GG × AA interactions	2129:2148	GG × AA interactions	2129:2148	Uptake and clearance of total EAA and group 2 AA were affected or tended to be affected by GG × AA interactions, with their uptakes being lower and their clearances higher with GG, but only at the LMP level.
31155242	1	4	dep	energy	224:229	arg1	response					212:219	response	212:219	response	212:219	We investigated mammary gland metabolism in lactating dairy cattle in response to energy from glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates at low (LMP) and high (HMP) metabolizable protein levels.
31155242	12	5	theme	×	2132:2132	arg1	interactions					2137:2148	GG × AA interactions	2129:2148	GG × AA interactions	2129:2148	Uptake and clearance of total EAA and group 2 AA were affected or tended to be affected by GG × AA interactions, with their uptakes being lower and their clearances higher with GG, but only at the LMP level.
31155242	7	6	theme	fat	1395:1397	arg1	yield					1399:1403	fat yield	1395:1403	fat yield	1395:1403	Neither GG nor LG infusion stimulated milk protein or lactose yield, but fat yield tended to decrease with GG and tended to increase with LG.
31155242	2	7	theme	AA	662:663	arg1	HMP-GG					741:746	HMP-GG	741:746	HMP-GG	741:746	According to a 6 × 6 Latin square design, 6 rumen-fistulated second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were abomasally infused with saline (LMP-C); isoenergetic infusions (digestible energy basis) of 1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA (EAA; HMP-C); or isoenergetic infusions of 1,319 g/d glucose + 844 g/d EAA (HMP-GG) or 676 g/d palm olein + 844 g/d EAA (HMP-LG).
31155242	2	7	theme	AA	662:663	arg1	infusions					545:553	isoenergetic infusions	532:553	isoenergetic infusions (digestible energy basis) of 1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA (EAA; HMP-C)	532:676	According to a 6 × 6 Latin square design, 6 rumen-fistulated second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were abomasally infused with saline (LMP-C); isoenergetic infusions (digestible energy basis) of 1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA (EAA; HMP-C); or isoenergetic infusions of 1,319 g/d glucose + 844 g/d EAA (HMP-GG) or 676 g/d palm olein + 844 g/d EAA (HMP-LG).
31155242	2	7	theme	AA	662:663	arg1	saline					516:521	saline	516:521	saline (LMP-C)	516:529	According to a 6 × 6 Latin square design, 6 rumen-fistulated second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were abomasally infused with saline (LMP-C); isoenergetic infusions (digestible energy basis) of 1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA (EAA; HMP-C); or isoenergetic infusions of 1,319 g/d glucose + 844 g/d EAA (HMP-GG) or 676 g/d palm olein + 844 g/d EAA (HMP-LG).
31155242	2	7	theme	AA	662:663	arg1	LMP-C					524:528	LMP-C	524:528	LMP-C	524:528	According to a 6 × 6 Latin square design, 6 rumen-fistulated second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were abomasally infused with saline (LMP-C); isoenergetic infusions (digestible energy basis) of 1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA (EAA; HMP-C); or isoenergetic infusions of 1,319 g/d glucose + 844 g/d EAA (HMP-GG) or 676 g/d palm olein + 844 g/d EAA (HMP-LG).
31155242	2	7	theme	AA	662:663	arg1	infusions					695:703	isoenergetic infusions	682:703	isoenergetic infusions of 1,319 g/d glucose	682:724	According to a 6 × 6 Latin square design, 6 rumen-fistulated second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were abomasally infused with saline (LMP-C); isoenergetic infusions (digestible energy basis) of 1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA (EAA; HMP-C); or isoenergetic infusions of 1,319 g/d glucose + 844 g/d EAA (HMP-GG) or 676 g/d palm olein + 844 g/d EAA (HMP-LG).
31155242	2	7	theme	AA	662:663	arg1	basis					574:578	digestible energy basis	556:578	digestible energy basis	556:578	According to a 6 × 6 Latin square design, 6 rumen-fistulated second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were abomasally infused with saline (LMP-C); isoenergetic infusions (digestible energy basis) of 1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA (EAA; HMP-C); or isoenergetic infusions of 1,319 g/d glucose + 844 g/d EAA (HMP-GG) or 676 g/d palm olein + 844 g/d EAA (HMP-LG).
31155242	2	8	theme	g/d	590:592	arg1	LMP-GG					603:608	LMP-GG	603:608	LMP-GG	603:608	According to a 6 × 6 Latin square design, 6 rumen-fistulated second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were abomasally infused with saline (LMP-C); isoenergetic infusions (digestible energy basis) of 1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA (EAA; HMP-C); or isoenergetic infusions of 1,319 g/d glucose + 844 g/d EAA (HMP-GG) or 676 g/d palm olein + 844 g/d EAA (HMP-LG).
31155242	2	8	theme	g/d	590:592	arg1	glucose					594:600	1,319 g/d glucose	584:600	1,319 g/d glucose (LMP-GG)	584:609	According to a 6 × 6 Latin square design, 6 rumen-fistulated second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were abomasally infused with saline (LMP-C); isoenergetic infusions (digestible energy basis) of 1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA (EAA; HMP-C); or isoenergetic infusions of 1,319 g/d glucose + 844 g/d EAA (HMP-GG) or 676 g/d palm olein + 844 g/d EAA (HMP-LG).
31155242	18	9	dep	lactose	2913:2919	arg1	synthesis					2929:2937	synthesis	2929:2937	synthesis	2929:2937	Mammary glucose uptake did not cover estimated requirements for lactose and fat synthesis at the HMP level, except during HMP-GG infusion.
31155242	10	10	theme	HMP	1828:1830	arg1	level					1832:1836	the HMP level	1824:1836	the HMP level	1824:1836	Compared with the LMP level, the HMP level increased arterial concentrations of BHB, urea, and all EAA groups and decreased the concentration of total non-EAA.
31155242	2	11	dep	EAA	666:668	arg1	HMP-C					671:675	HMP-C	671:675	HMP-C	671:675	According to a 6 × 6 Latin square design, 6 rumen-fistulated second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were abomasally infused with saline (LMP-C); isoenergetic infusions (digestible energy basis) of 1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA (EAA; HMP-C); or isoenergetic infusions of 1,319 g/d glucose + 844 g/d EAA (HMP-GG) or 676 g/d palm olein + 844 g/d EAA (HMP-LG).
31155242	2	12	theme	palm	620:623	arg1	LMP-LG					632:637	LMP-LG	632:637	LMP-LG	632:637	According to a 6 × 6 Latin square design, 6 rumen-fistulated second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were abomasally infused with saline (LMP-C); isoenergetic infusions (digestible energy basis) of 1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA (EAA; HMP-C); or isoenergetic infusions of 1,319 g/d glucose + 844 g/d EAA (HMP-GG) or 676 g/d palm olein + 844 g/d EAA (HMP-LG).
31155242	2	12	theme	palm	620:623	arg1	olein					625:629	676 g/d palm olein	612:629	676 g/d palm olein (LMP-LG)	612:638	According to a 6 × 6 Latin square design, 6 rumen-fistulated second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were abomasally infused with saline (LMP-C); isoenergetic infusions (digestible energy basis) of 1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA (EAA; HMP-C); or isoenergetic infusions of 1,319 g/d glucose + 844 g/d EAA (HMP-GG) or 676 g/d palm olein + 844 g/d EAA (HMP-LG).
31155242	14	13	theme	groups	2379:2384	arg1	uptake					2337:2342	uptake	2337:2342	uptake	2337:2342	The HMP level increased uptake and decreased clearance of all EAA groups and decreased non-EAA uptake.
31155242	14	13	theme	groups	2379:2384	arg1	clearance					2358:2366	decreased clearance	2348:2366	decreased clearance	2348:2366	The HMP level increased uptake and decreased clearance of all EAA groups and decreased non-EAA uptake.
31155242	0	14	theme	Mammary	0:6	arg1	utilization					25:35	Mammary gland metabolite utilization	0:35	Mammary gland metabolite utilization in response to exogenous glucose or long-chain fatty acids at low and high metabolizable protein levels.	0:140	Mammary gland metabolite utilization in response to exogenous glucose or long-chain fatty acids at low and high metabolizable protein levels.
31155242	13	15	theme	group	2306:2310	arg1	uptake					2276:2281	uptake	2276:2281	uptake	2276:2281	Infusion of LG did not affect uptake or clearance of any AA group.
31155242	13	15	theme	group	2306:2310	arg1	clearance					2286:2294	clearance	2286:2294	clearance	2286:2294	Infusion of LG did not affect uptake or clearance of any AA group.
31155242	3	16	theme	experimental	800:811	arg1	period					813:818	Each experimental period	795:818	Each experimental period	795:818	Each experimental period consisted of 5 d of continuous infusion followed by 2 d of rest.
31155242	2	17	theme	676	612:614	arg1	LMP-LG					632:637	LMP-LG	632:637	LMP-LG	632:637	According to a 6 × 6 Latin square design, 6 rumen-fistulated second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were abomasally infused with saline (LMP-C); isoenergetic infusions (digestible energy basis) of 1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA (EAA; HMP-C); or isoenergetic infusions of 1,319 g/d glucose + 844 g/d EAA (HMP-GG) or 676 g/d palm olein + 844 g/d EAA (HMP-LG).
31155242	2	17	theme	676	612:614	arg1	olein					625:629	676 g/d palm olein	612:629	676 g/d palm olein (LMP-LG)	612:638	According to a 6 × 6 Latin square design, 6 rumen-fistulated second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were abomasally infused with saline (LMP-C); isoenergetic infusions (digestible energy basis) of 1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA (EAA; HMP-C); or isoenergetic infusions of 1,319 g/d glucose + 844 g/d EAA (HMP-GG) or 676 g/d palm olein + 844 g/d EAA (HMP-LG).
31155242	9	18	theme	LG	1701:1702	arg1	Infusion					1689:1696	Infusion	1689:1696	Infusion of LG	1689:1702	Infusion of LG increased arterial triacylglycerides (TAG) and LCFA but did not affect EAA concentrations.
31155242	1	19	theme	lactating	186:194	arg1	cattle					202:207	lactating dairy cattle	186:207	lactating dairy cattle	186:207	We investigated mammary gland metabolism in lactating dairy cattle in response to energy from glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates at low (LMP) and high (HMP) metabolizable protein levels.
31155242	4	20	theme	grass	928:932	arg1	silage					934:939	31% grass silage	924:939	31% grass silage	924:939	A total mixed ration (42% corn silage, 31% grass silage, and 27% concentrate on a dry matter basis) formulated to meet 100 and 83% of net energy and metabolizable protein requirements, respectively, was fed at 90% of ad libitum intake by individual cow.
31155242	9	21	theme	arterial	1714:1721	arg1	TAG					1742:1744	TAG	1742:1744	TAG	1742:1744	Infusion of LG increased arterial triacylglycerides (TAG) and LCFA but did not affect EAA concentrations.
31155242	9	21	theme	arterial	1714:1721	arg1	triacylglycerides					1723:1739	arterial triacylglycerides	1714:1739	arterial triacylglycerides (TAG)	1714:1745	Infusion of LG increased arterial triacylglycerides (TAG) and LCFA but did not affect EAA concentrations.
31155242	0	22	theme	high	107:110	arg1	levels					134:139	low and high metabolizable protein levels	99:139	low and high metabolizable protein levels	99:139	Mammary gland metabolite utilization in response to exogenous glucose or long-chain fatty acids at low and high metabolizable protein levels.
31155242	1	23	theme	high	319:322	arg1	HMP					325:327	HMP	325:327	HMP	325:327	We investigated mammary gland metabolism in lactating dairy cattle in response to energy from glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates at low (LMP) and high (HMP) metabolizable protein levels.
31155242	1	23	theme	high	319:322	arg1	levels					352:357	low (LMP) and high (HMP) metabolizable protein levels	305:357	low (LMP) and high (HMP) metabolizable protein levels	305:357	We investigated mammary gland metabolism in lactating dairy cattle in response to energy from glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates at low (LMP) and high (HMP) metabolizable protein levels.
31155242	1	24	dep	glucogenic	236:245	arg1	glucose					248:254	glucose	248:254	glucose	248:254	We investigated mammary gland metabolism in lactating dairy cattle in response to energy from glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates at low (LMP) and high (HMP) metabolizable protein levels.
31155242	1	24	dep	glucogenic	236:245	arg1	olein					280:284	palm olein	275:284	palm olein; LG	275:288	We investigated mammary gland metabolism in lactating dairy cattle in response to energy from glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates at low (LMP) and high (HMP) metabolizable protein levels.
31155242	11	25	theme	protein	2023:2029	arg1	level					2031:2035	protein level	2023:2035	protein level	2023:2035	Mammary plasma flow increased with GG and was not affected by LG or protein level.
31155242	4	26	theme	energy	1023:1028	arg1	requirements					1056:1067	metabolizable protein requirements	1034:1067	metabolizable protein requirements	1034:1067	A total mixed ration (42% corn silage, 31% grass silage, and 27% concentrate on a dry matter basis) formulated to meet 100 and 83% of net energy and metabolizable protein requirements, respectively, was fed at 90% of ad libitum intake by individual cow.
31155242	4	26	theme	energy	1023:1028	arg1	%					1014:1014	100 and 83%	1004:1014	100 and 83% of net energy	1004:1028	A total mixed ration (42% corn silage, 31% grass silage, and 27% concentrate on a dry matter basis) formulated to meet 100 and 83% of net energy and metabolizable protein requirements, respectively, was fed at 90% of ad libitum intake by individual cow.
31155242	4	26	theme	energy	1023:1028	arg1	energy					1023:1028	net energy	1019:1028	net energy	1019:1028	A total mixed ration (42% corn silage, 31% grass silage, and 27% concentrate on a dry matter basis) formulated to meet 100 and 83% of net energy and metabolizable protein requirements, respectively, was fed at 90% of ad libitum intake by individual cow.
31155242	0	27	theme	protein	126:132	arg1	levels					134:139	low and high metabolizable protein levels	99:139	low and high metabolizable protein levels	99:139	Mammary gland metabolite utilization in response to exogenous glucose or long-chain fatty acids at low and high metabolizable protein levels.
31155242	19	28	theme	aminogenic	3120:3129	arg1	substrate					3131:3139	glucogenic, lipogenic, and aminogenic substrate	3093:3139	glucogenic, lipogenic, and aminogenic substrate	3093:3139	Results of this study illustrate flexibility in mammary metabolite utilization when absorptive supply of glucogenic, lipogenic, and aminogenic substrate is increased.
31155242	10	29	theme	urea	1880:1883	arg1	concentrations					1857:1870	arterial concentrations	1848:1870	arterial concentrations of BHB, urea, and all EAA groups	1848:1903	Compared with the LMP level, the HMP level increased arterial concentrations of BHB, urea, and all EAA groups and decreased the concentration of total non-EAA.
31155242	2	30	theme	g/d	777:779	arg1	HMP-LG					786:791	HMP-LG	786:791	HMP-LG	786:791	According to a 6 × 6 Latin square design, 6 rumen-fistulated second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were abomasally infused with saline (LMP-C); isoenergetic infusions (digestible energy basis) of 1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA (EAA; HMP-C); or isoenergetic infusions of 1,319 g/d glucose + 844 g/d EAA (HMP-GG) or 676 g/d palm olein + 844 g/d EAA (HMP-LG).
31155242	2	30	theme	g/d	777:779	arg1	EAA					781:783	844 g/d EAA	773:783	844 g/d EAA (HMP-LG)	773:792	According to a 6 × 6 Latin square design, 6 rumen-fistulated second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were abomasally infused with saline (LMP-C); isoenergetic infusions (digestible energy basis) of 1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA (EAA; HMP-C); or isoenergetic infusions of 1,319 g/d glucose + 844 g/d EAA (HMP-GG) or 676 g/d palm olein + 844 g/d EAA (HMP-LG).
31155242	14	31	theme	non-EAA	2400:2406	arg1	uptake					2408:2413	non-EAA uptake	2400:2413	non-EAA uptake	2400:2413	The HMP level increased uptake and decreased clearance of all EAA groups and decreased non-EAA uptake.
31155242	4	32	theme	42	907:908	arg1	%					909:909	%	909:909	%	909:909	A total mixed ration (42% corn silage, 31% grass silage, and 27% concentrate on a dry matter basis) formulated to meet 100 and 83% of net energy and metabolizable protein requirements, respectively, was fed at 90% of ad libitum intake by individual cow.
31155242	4	33	theme	metabolizable	1034:1046	arg1	requirements					1056:1067	metabolizable protein requirements	1034:1067	metabolizable protein requirements	1034:1067	A total mixed ration (42% corn silage, 31% grass silage, and 27% concentrate on a dry matter basis) formulated to meet 100 and 83% of net energy and metabolizable protein requirements, respectively, was fed at 90% of ad libitum intake by individual cow.
31155242	1	34	theme	metabolizable	330:342	arg1	HMP					325:327	HMP	325:327	HMP	325:327	We investigated mammary gland metabolism in lactating dairy cattle in response to energy from glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates at low (LMP) and high (HMP) metabolizable protein levels.
31155242	1	34	theme	metabolizable	330:342	arg1	levels					352:357	low (LMP) and high (HMP) metabolizable protein levels	305:357	low (LMP) and high (HMP) metabolizable protein levels	305:357	We investigated mammary gland metabolism in lactating dairy cattle in response to energy from glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates at low (LMP) and high (HMP) metabolizable protein levels.
31155242	4	35	theme	%	926:926	arg1	silage					934:939	31% grass silage	924:939	31% grass silage	924:939	A total mixed ration (42% corn silage, 31% grass silage, and 27% concentrate on a dry matter basis) formulated to meet 100 and 83% of net energy and metabolizable protein requirements, respectively, was fed at 90% of ad libitum intake by individual cow.
31155242	10	36	theme	BHB	1875:1877	arg1	concentrations					1857:1870	arterial concentrations	1848:1870	arterial concentrations of BHB, urea, and all EAA groups	1848:1903	Compared with the LMP level, the HMP level increased arterial concentrations of BHB, urea, and all EAA groups and decreased the concentration of total non-EAA.
31155242	4	37	theme	corn	911:914	arg1	silage					916:921	42% corn silage	907:921	42% corn silage	907:921	A total mixed ration (42% corn silage, 31% grass silage, and 27% concentrate on a dry matter basis) formulated to meet 100 and 83% of net energy and metabolizable protein requirements, respectively, was fed at 90% of ad libitum intake by individual cow.
31155242	2	38	theme	rumen-fistulated	404:419	arg1	cows					462:465	6 rumen-fistulated second-lactation Holstein-Friesian dairy cows	402:465	6 rumen-fistulated second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk)	402:485	According to a 6 × 6 Latin square design, 6 rumen-fistulated second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were abomasally infused with saline (LMP-C); isoenergetic infusions (digestible energy basis) of 1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA (EAA; HMP-C); or isoenergetic infusions of 1,319 g/d glucose + 844 g/d EAA (HMP-GG) or 676 g/d palm olein + 844 g/d EAA (HMP-LG).
31155242	1	39	dep	glucose	248:254	arg1	GG					257:258	GG	257:258	GG	257:258	We investigated mammary gland metabolism in lactating dairy cattle in response to energy from glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates at low (LMP) and high (HMP) metabolizable protein levels.
31155242	4	40	theme	total	887:891	arg1	ration					899:904	A total mixed ration	885:904	A total mixed ration (42% corn silage, 31% grass silage, and 27% concentrate on a dry matter basis)	885:983	A total mixed ration (42% corn silage, 31% grass silage, and 27% concentrate on a dry matter basis) formulated to meet 100 and 83% of net energy and metabolizable protein requirements, respectively, was fed at 90% of ad libitum intake by individual cow.
31155242	17	41	from	lipogenesis	2756:2766	arg1	adipose					2771:2777	adipose	2771:2777	adipose	2771:2777	We suspect GG increased mammary plasma flow to maintain intramammary energy and AA balance and stimulated lipogenesis in adipose, accounting for depressed arterial BHB and group 2 AA concentrations.
31155242	17	42	theme	intramammary	2706:2717	arg1	energy					2719:2724	intramammary energy and AA balance	2706:2739	energy	2719:2724	We suspect GG increased mammary plasma flow to maintain intramammary energy and AA balance and stimulated lipogenesis in adipose, accounting for depressed arterial BHB and group 2 AA concentrations.
31155242	4	43	theme	ad	1102:1103	arg1	intake					1113:1118	ad libitum intake	1102:1118	ad libitum intake	1102:1118	A total mixed ration (42% corn silage, 31% grass silage, and 27% concentrate on a dry matter basis) formulated to meet 100 and 83% of net energy and metabolizable protein requirements, respectively, was fed at 90% of ad libitum intake by individual cow.
31155242	8	44	theme	fatty	1614:1618	arg1	acids					1620:1624	nonesterified fatty acids	1600:1624	nonesterified fatty acids	1600:1624	Infusion of GG increased arterial plasma concentrations of glucose and insulin and decreased concentrations of β-hydroxybutyrate (BHB), nonesterified fatty acids, long-chain fatty acids (LCFA), total AA, EAA, and group 2 AA.
31155242	4	45	dep	%	948:948	arg1	concentrate					950:960	concentrate	950:960	concentrate on a dry matter basis	950:982	A total mixed ration (42% corn silage, 31% grass silage, and 27% concentrate on a dry matter basis) formulated to meet 100 and 83% of net energy and metabolizable protein requirements, respectively, was fed at 90% of ad libitum intake by individual cow.
31155242	19	46	theme	metabolite	3044:3053	arg1	utilization					3055:3065	mammary metabolite utilization	3036:3065	mammary metabolite utilization	3036:3065	Results of this study illustrate flexibility in mammary metabolite utilization when absorptive supply of glucogenic, lipogenic, and aminogenic substrate is increased.
31155242	2	47	theme	676	752:754	arg1	olein					765:769	676 g/d palm olein	752:769	676 g/d palm olein	752:769	According to a 6 × 6 Latin square design, 6 rumen-fistulated second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were abomasally infused with saline (LMP-C); isoenergetic infusions (digestible energy basis) of 1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA (EAA; HMP-C); or isoenergetic infusions of 1,319 g/d glucose + 844 g/d EAA (HMP-GG) or 676 g/d palm olein + 844 g/d EAA (HMP-LG).
31155242	11	48	theme	Mammary	1955:1961	arg1	flow					1970:1973	Mammary plasma flow	1955:1973	Mammary plasma flow	1955:1973	Mammary plasma flow increased with GG and was not affected by LG or protein level.
31155242	0	49	theme	long-chain	73:82	arg1	acids					90:94	long-chain fatty acids	73:94	long-chain fatty acids	73:94	Mammary gland metabolite utilization in response to exogenous glucose or long-chain fatty acids at low and high metabolizable protein levels.
31155242	6	50	theme	milk	1261:1264	arg1	yield					1266:1270	milk yield	1261:1270	milk yield	1261:1270	Infusing GG or LG at the HMP level did not affect milk yield or composition differently than at the LMP level.
31155242	1	51	theme	mammary	158:164	arg1	metabolism					172:181	mammary gland metabolism	158:181	mammary gland metabolism in lactating dairy cattle	158:207	We investigated mammary gland metabolism in lactating dairy cattle in response to energy from glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates at low (LMP) and high (HMP) metabolizable protein levels.
31155242	19	52	theme	glucogenic	3093:3102	arg1	substrate					3131:3139	glucogenic, lipogenic, and aminogenic substrate	3093:3139	glucogenic, lipogenic, and aminogenic substrate	3093:3139	Results of this study illustrate flexibility in mammary metabolite utilization when absorptive supply of glucogenic, lipogenic, and aminogenic substrate is increased.
31155242	5	53	theme	Arterial	1139:1146	arg1	samples					1165:1171	Arterial and venous blood samples	1139:1171	Arterial and venous blood samples	1139:1171	Arterial and venous blood samples were collected on d 5 of each period.
31155242	12	54	theme	group	2076:2080	arg1	AA					2084:2085	total EAA and group 2 AA	2062:2085	AA	2084:2085	Uptake and clearance of total EAA and group 2 AA were affected or tended to be affected by GG × AA interactions, with their uptakes being lower and their clearances higher with GG, but only at the LMP level.
31155242	8	55	theme	β-hydroxybutyrate	1575:1591	arg1	concentrations					1557:1570	concentrations	1557:1570	concentrations of β-hydroxybutyrate (BHB), nonesterified fatty acids, long-chain fatty acids (LCFA), total AA, EAA, and group 2 AA	1557:1686	Infusion of GG increased arterial plasma concentrations of glucose and insulin and decreased concentrations of β-hydroxybutyrate (BHB), nonesterified fatty acids, long-chain fatty acids (LCFA), total AA, EAA, and group 2 AA.
31155242	16	56	theme	TAG	2574:2576	arg1	uptake					2564:2569	mammary uptake	2556:2569	mammary uptake of TAG and LCFA	2556:2585	Infusion of LG increased mammary uptake of TAG and LCFA and increased or tended to increase clearance of TAG and LCFA.
31155242	15	57	theme	LMP	2520:2522	arg1	level					2524:2528	the LMP level	2516:2528	the LMP level	2516:2528	Infusion of GG tended to increase mammary glucose uptake, and tended to decrease BHB uptake only at the LMP level.
31155242	2	58	theme	Holstein-Friesian	438:454	arg1	cows					462:465	6 rumen-fistulated second-lactation Holstein-Friesian dairy cows	402:465	6 rumen-fistulated second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk)	402:485	According to a 6 × 6 Latin square design, 6 rumen-fistulated second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were abomasally infused with saline (LMP-C); isoenergetic infusions (digestible energy basis) of 1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA (EAA; HMP-C); or isoenergetic infusions of 1,319 g/d glucose + 844 g/d EAA (HMP-GG) or 676 g/d palm olein + 844 g/d EAA (HMP-LG).
31155242	17	59	dep	BHB	2814:2816	arg1	concentrations					2833:2846	AA concentrations	2830:2846	AA concentrations	2830:2846	We suspect GG increased mammary plasma flow to maintain intramammary energy and AA balance and stimulated lipogenesis in adipose, accounting for depressed arterial BHB and group 2 AA concentrations.
31155242	12	60	theme	AA	2084:2085	arg1	Uptake					2038:2043	Uptake	2038:2043	Uptake	2038:2043	Uptake and clearance of total EAA and group 2 AA were affected or tended to be affected by GG × AA interactions, with their uptakes being lower and their clearances higher with GG, but only at the LMP level.
31155242	12	60	theme	AA	2084:2085	arg1	clearance					2049:2057	clearance	2049:2057	clearance	2049:2057	Uptake and clearance of total EAA and group 2 AA were affected or tended to be affected by GG × AA interactions, with their uptakes being lower and their clearances higher with GG, but only at the LMP level.
31155242	0	61	theme	low	99:101	arg1	levels					134:139	low and high metabolizable protein levels	99:139	low and high metabolizable protein levels	99:139	Mammary gland metabolite utilization in response to exogenous glucose or long-chain fatty acids at low and high metabolizable protein levels.
31155242	3	62	theme	infusion	851:858	arg1	d					835:835	5 d	833:835	5 d of continuous infusion	833:858	Each experimental period consisted of 5 d of continuous infusion followed by 2 d of rest.
31155242	16	63	theme	LCFA	2582:2585	arg1	uptake					2564:2569	mammary uptake	2556:2569	mammary uptake of TAG and LCFA	2556:2585	Infusion of LG increased mammary uptake of TAG and LCFA and increased or tended to increase clearance of TAG and LCFA.
31155242	10	64	theme	non-EAA	1946:1952	arg1	concentration					1923:1935	the concentration	1919:1935	the concentration of total non-EAA	1919:1952	Compared with the LMP level, the HMP level increased arterial concentrations of BHB, urea, and all EAA groups and decreased the concentration of total non-EAA.
31155242	1	65	dep	olein	280:284	arg1	LG					287:288	LG	287:288	palm olein; LG	275:288	We investigated mammary gland metabolism in lactating dairy cattle in response to energy from glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates at low (LMP) and high (HMP) metabolizable protein levels.
31155242	10	66	theme	EAA	1894:1896	arg1	groups					1898:1903	all EAA groups	1890:1903	all EAA groups	1890:1903	Compared with the LMP level, the HMP level increased arterial concentrations of BHB, urea, and all EAA groups and decreased the concentration of total non-EAA.
31155242	3	67	dep	consisted	820:828	arg1	followed					860:867	followed	860:867	followed by 2 d of rest	860:882	Each experimental period consisted of 5 d of continuous infusion followed by 2 d of rest.
31155242	19	68	theme	lipogenic	3105:3113	arg1	substrate					3131:3139	glucogenic, lipogenic, and aminogenic substrate	3093:3139	glucogenic, lipogenic, and aminogenic substrate	3093:3139	Results of this study illustrate flexibility in mammary metabolite utilization when absorptive supply of glucogenic, lipogenic, and aminogenic substrate is increased.
31155242	1	69	dep	low	305:307	arg1	LMP					310:312	LMP	310:312	LMP	310:312	We investigated mammary gland metabolism in lactating dairy cattle in response to energy from glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates at low (LMP) and high (HMP) metabolizable protein levels.
31155242	2	70	theme	digestible	556:565	arg1	infusions					545:553	isoenergetic infusions	532:553	isoenergetic infusions (digestible energy basis) of 1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA (EAA; HMP-C)	532:676	According to a 6 × 6 Latin square design, 6 rumen-fistulated second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were abomasally infused with saline (LMP-C); isoenergetic infusions (digestible energy basis) of 1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA (EAA; HMP-C); or isoenergetic infusions of 1,319 g/d glucose + 844 g/d EAA (HMP-GG) or 676 g/d palm olein + 844 g/d EAA (HMP-LG).
31155242	2	70	theme	digestible	556:565	arg1	basis					574:578	digestible energy basis	556:578	digestible energy basis	556:578	According to a 6 × 6 Latin square design, 6 rumen-fistulated second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were abomasally infused with saline (LMP-C); isoenergetic infusions (digestible energy basis) of 1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA (EAA; HMP-C); or isoenergetic infusions of 1,319 g/d glucose + 844 g/d EAA (HMP-GG) or 676 g/d palm olein + 844 g/d EAA (HMP-LG).
31155242	4	71	theme	intake	1113:1118	arg1	%					1097:1097	90%	1095:1097	90% of ad libitum intake	1095:1118	A total mixed ration (42% corn silage, 31% grass silage, and 27% concentrate on a dry matter basis) formulated to meet 100 and 83% of net energy and metabolizable protein requirements, respectively, was fed at 90% of ad libitum intake by individual cow.
31155242	4	71	theme	intake	1113:1118	arg1	intake					1113:1118	ad libitum intake	1102:1118	ad libitum intake	1102:1118	A total mixed ration (42% corn silage, 31% grass silage, and 27% concentrate on a dry matter basis) formulated to meet 100 and 83% of net energy and metabolizable protein requirements, respectively, was fed at 90% of ad libitum intake by individual cow.
31155242	0	72	theme	metabolite	14:23	arg1	utilization					25:35	Mammary gland metabolite utilization	0:35	Mammary gland metabolite utilization in response to exogenous glucose or long-chain fatty acids at low and high metabolizable protein levels.	0:140	Mammary gland metabolite utilization in response to exogenous glucose or long-chain fatty acids at low and high metabolizable protein levels.
31155242	1	73	theme	lipogenic	264:272	arg1	substrates					291:300	glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates	236:300	glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates	236:300	We investigated mammary gland metabolism in lactating dairy cattle in response to energy from glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates at low (LMP) and high (HMP) metabolizable protein levels.
31155242	0	74	dep	glucose	62:68	arg1	response					40:47	response	40:47	response	40:47	Mammary gland metabolite utilization in response to exogenous glucose or long-chain fatty acids at low and high metabolizable protein levels.
31155242	8	75	theme	total	1658:1662	arg1	AA					1664:1665	total AA	1658:1665	total AA	1658:1665	Infusion of GG increased arterial plasma concentrations of glucose and insulin and decreased concentrations of β-hydroxybutyrate (BHB), nonesterified fatty acids, long-chain fatty acids (LCFA), total AA, EAA, and group 2 AA.
31155242	2	76	theme	square	387:392	arg1	design					394:399	a 6 × 6 Latin square design	373:399	a 6 × 6 Latin square design	373:399	According to a 6 × 6 Latin square design, 6 rumen-fistulated second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were abomasally infused with saline (LMP-C); isoenergetic infusions (digestible energy basis) of 1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA (EAA; HMP-C); or isoenergetic infusions of 1,319 g/d glucose + 844 g/d EAA (HMP-GG) or 676 g/d palm olein + 844 g/d EAA (HMP-LG).
31155242	2	77	theme	g/d	714:716	arg1	glucose					718:724	1,319 g/d glucose	708:724	1,319 g/d glucose	708:724	According to a 6 × 6 Latin square design, 6 rumen-fistulated second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were abomasally infused with saline (LMP-C); isoenergetic infusions (digestible energy basis) of 1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA (EAA; HMP-C); or isoenergetic infusions of 1,319 g/d glucose + 844 g/d EAA (HMP-GG) or 676 g/d palm olein + 844 g/d EAA (HMP-LG).
31155242	4	78	theme	dry	967:969	arg1	basis					978:982	a dry matter basis	965:982	a dry matter basis	965:982	A total mixed ration (42% corn silage, 31% grass silage, and 27% concentrate on a dry matter basis) formulated to meet 100 and 83% of net energy and metabolizable protein requirements, respectively, was fed at 90% of ad libitum intake by individual cow.
31155242	17	79	theme	plasma	2682:2687	arg1	flow					2689:2692	mammary plasma flow	2674:2692	mammary plasma flow to maintain intramammary energy and AA balance	2674:2739	We suspect GG increased mammary plasma flow to maintain intramammary energy and AA balance and stimulated lipogenesis in adipose, accounting for depressed arterial BHB and group 2 AA concentrations.
31155242	4	80	theme	individual	1123:1132	arg1	cow					1134:1136	individual cow	1123:1136	individual cow	1123:1136	A total mixed ration (42% corn silage, 31% grass silage, and 27% concentrate on a dry matter basis) formulated to meet 100 and 83% of net energy and metabolizable protein requirements, respectively, was fed at 90% of ad libitum intake by individual cow.
31155242	1	81	theme	palm	275:278	arg1	olein					280:284	palm olein	275:284	palm olein; LG	275:288	We investigated mammary gland metabolism in lactating dairy cattle in response to energy from glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates at low (LMP) and high (HMP) metabolizable protein levels.
31155242	19	82	theme	substrate	3131:3139	arg1	supply					3083:3088	absorptive supply	3072:3088	absorptive supply of glucogenic, lipogenic, and aminogenic substrate	3072:3139	Results of this study illustrate flexibility in mammary metabolite utilization when absorptive supply of glucogenic, lipogenic, and aminogenic substrate is increased.
31155242	5	83	theme	venous	1152:1157	arg1	samples					1165:1171	Arterial and venous blood samples	1139:1171	Arterial and venous blood samples	1139:1171	Arterial and venous blood samples were collected on d 5 of each period.
31155242	17	84	theme	arterial	2805:2812	arg1	BHB					2814:2816	depressed arterial BHB	2795:2816	depressed arterial BHB	2795:2816	We suspect GG increased mammary plasma flow to maintain intramammary energy and AA balance and stimulated lipogenesis in adipose, accounting for depressed arterial BHB and group 2 AA concentrations.
31155242	1	85	from	substrates	291:300	arg1	energy					224:229	energy	224:229	energy from glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates at low (LMP) and high (HMP) metabolizable protein levels	224:357	We investigated mammary gland metabolism in lactating dairy cattle in response to energy from glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates at low (LMP) and high (HMP) metabolizable protein levels.
31155242	12	86	theme	LMP	2235:2237	arg1	level					2239:2243	the LMP level	2231:2243	the LMP level	2231:2243	Uptake and clearance of total EAA and group 2 AA were affected or tended to be affected by GG × AA interactions, with their uptakes being lower and their clearances higher with GG, but only at the LMP level.
31155242	2	87	theme	essential	652:660	arg1	EAA					666:668	EAA	666:668	EAA	666:668	According to a 6 × 6 Latin square design, 6 rumen-fistulated second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were abomasally infused with saline (LMP-C); isoenergetic infusions (digestible energy basis) of 1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA (EAA; HMP-C); or isoenergetic infusions of 1,319 g/d glucose + 844 g/d EAA (HMP-GG) or 676 g/d palm olein + 844 g/d EAA (HMP-LG).
31155242	2	87	theme	essential	652:660	arg1	AA					662:663	1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA	584:663	1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA (EAA; HMP-C)	584:676	According to a 6 × 6 Latin square design, 6 rumen-fistulated second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were abomasally infused with saline (LMP-C); isoenergetic infusions (digestible energy basis) of 1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA (EAA; HMP-C); or isoenergetic infusions of 1,319 g/d glucose + 844 g/d EAA (HMP-GG) or 676 g/d palm olein + 844 g/d EAA (HMP-LG).
31155242	2	88	theme	g/d	732:734	arg1	EAA					736:738	844 g/d EAA	728:738	844 g/d EAA	728:738	According to a 6 × 6 Latin square design, 6 rumen-fistulated second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were abomasally infused with saline (LMP-C); isoenergetic infusions (digestible energy basis) of 1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA (EAA; HMP-C); or isoenergetic infusions of 1,319 g/d glucose + 844 g/d EAA (HMP-GG) or 676 g/d palm olein + 844 g/d EAA (HMP-LG).
31155242	14	89	theme	HMP	2317:2319	arg1	level					2321:2325	The HMP level	2313:2325	The HMP level	2313:2325	The HMP level increased uptake and decreased clearance of all EAA groups and decreased non-EAA uptake.
31155242	3	90	theme	rest	879:882	arg1	d					874:874	2 d	872:874	2 d of rest	872:882	Each experimental period consisted of 5 d of continuous infusion followed by 2 d of rest.
31155242	8	91	theme	group	1677:1681	arg1	AA					1685:1686	group 2 AA	1677:1686	group 2 AA	1677:1686	Infusion of GG increased arterial plasma concentrations of glucose and insulin and decreased concentrations of β-hydroxybutyrate (BHB), nonesterified fatty acids, long-chain fatty acids (LCFA), total AA, EAA, and group 2 AA.
31155242	18	92	theme	Mammary	2849:2855	arg1	uptake					2865:2870	Mammary glucose uptake	2849:2870	Mammary glucose uptake	2849:2870	Mammary glucose uptake did not cover estimated requirements for lactose and fat synthesis at the HMP level, except during HMP-GG infusion.
31155242	12	93	theme	EAA	2068:2070	arg1	Uptake					2038:2043	Uptake	2038:2043	Uptake	2038:2043	Uptake and clearance of total EAA and group 2 AA were affected or tended to be affected by GG × AA interactions, with their uptakes being lower and their clearances higher with GG, but only at the LMP level.
31155242	12	93	theme	EAA	2068:2070	arg1	clearance					2049:2057	clearance	2049:2057	clearance	2049:2057	Uptake and clearance of total EAA and group 2 AA were affected or tended to be affected by GG × AA interactions, with their uptakes being lower and their clearances higher with GG, but only at the LMP level.
31155242	8	94	theme	acids	1644:1648	arg1	concentrations					1557:1570	concentrations	1557:1570	concentrations of β-hydroxybutyrate (BHB), nonesterified fatty acids, long-chain fatty acids (LCFA), total AA, EAA, and group 2 AA	1557:1686	Infusion of GG increased arterial plasma concentrations of glucose and insulin and decreased concentrations of β-hydroxybutyrate (BHB), nonesterified fatty acids, long-chain fatty acids (LCFA), total AA, EAA, and group 2 AA.
31155242	8	95	theme	AA	1685:1686	arg1	concentrations					1557:1570	concentrations	1557:1570	concentrations of β-hydroxybutyrate (BHB), nonesterified fatty acids, long-chain fatty acids (LCFA), total AA, EAA, and group 2 AA	1557:1686	Infusion of GG increased arterial plasma concentrations of glucose and insulin and decreased concentrations of β-hydroxybutyrate (BHB), nonesterified fatty acids, long-chain fatty acids (LCFA), total AA, EAA, and group 2 AA.
31155242	14	96	theme	EAA	2375:2377	arg1	groups					2379:2384	all EAA groups	2371:2384	all EAA groups	2371:2384	The HMP level increased uptake and decreased clearance of all EAA groups and decreased non-EAA uptake.
31155242	16	97	theme	LCFA	2644:2647	arg1	clearance					2623:2631	clearance	2623:2631	clearance of TAG and LCFA	2623:2647	Infusion of LG increased mammary uptake of TAG and LCFA and increased or tended to increase clearance of TAG and LCFA.
31155242	2	98	from	d	476:476	arg1	milk					481:484	milk	481:484	milk	481:484	According to a 6 × 6 Latin square design, 6 rumen-fistulated second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were abomasally infused with saline (LMP-C); isoenergetic infusions (digestible energy basis) of 1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA (EAA; HMP-C); or isoenergetic infusions of 1,319 g/d glucose + 844 g/d EAA (HMP-GG) or 676 g/d palm olein + 844 g/d EAA (HMP-LG).
31155242	7	99	theme	lactose	1376:1382	arg1	yield					1384:1388	milk protein or lactose yield	1360:1388	yield	1384:1388	Neither GG nor LG infusion stimulated milk protein or lactose yield, but fat yield tended to decrease with GG and tended to increase with LG.
31155242	16	100	theme	TAG	2636:2638	arg1	clearance					2623:2631	clearance	2623:2631	clearance of TAG and LCFA	2623:2647	Infusion of LG increased mammary uptake of TAG and LCFA and increased or tended to increase clearance of TAG and LCFA.
31155242	2	101	theme	olein	625:629	arg1	EAA					666:668	EAA	666:668	EAA	666:668	According to a 6 × 6 Latin square design, 6 rumen-fistulated second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were abomasally infused with saline (LMP-C); isoenergetic infusions (digestible energy basis) of 1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA (EAA; HMP-C); or isoenergetic infusions of 1,319 g/d glucose + 844 g/d EAA (HMP-GG) or 676 g/d palm olein + 844 g/d EAA (HMP-LG).
31155242	2	101	theme	olein	625:629	arg1	AA					662:663	1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA	584:663	1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA (EAA; HMP-C)	584:676	According to a 6 × 6 Latin square design, 6 rumen-fistulated second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were abomasally infused with saline (LMP-C); isoenergetic infusions (digestible energy basis) of 1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA (EAA; HMP-C); or isoenergetic infusions of 1,319 g/d glucose + 844 g/d EAA (HMP-GG) or 676 g/d palm olein + 844 g/d EAA (HMP-LG).
31155242	2	102	theme	isoenergetic	682:693	arg1	infusions					695:703	isoenergetic infusions	682:703	isoenergetic infusions of 1,319 g/d glucose	682:724	According to a 6 × 6 Latin square design, 6 rumen-fistulated second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were abomasally infused with saline (LMP-C); isoenergetic infusions (digestible energy basis) of 1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA (EAA; HMP-C); or isoenergetic infusions of 1,319 g/d glucose + 844 g/d EAA (HMP-GG) or 676 g/d palm olein + 844 g/d EAA (HMP-LG).
31155242	5	103	theme	period	1203:1208	arg1	d					1191:1191	d 5	1191:1193	d 5 of each period	1191:1208	Arterial and venous blood samples were collected on d 5 of each period.
31155242	14	104	theme	decreased	2348:2356	arg1	clearance					2358:2366	decreased clearance	2348:2366	decreased clearance	2348:2366	The HMP level increased uptake and decreased clearance of all EAA groups and decreased non-EAA uptake.
31155242	4	105	dep	ration	899:904	arg1	%					948:948	27%	946:948	27% concentrate on a dry matter basis	946:982	A total mixed ration (42% corn silage, 31% grass silage, and 27% concentrate on a dry matter basis) formulated to meet 100 and 83% of net energy and metabolizable protein requirements, respectively, was fed at 90% of ad libitum intake by individual cow.
31155242	4	105	dep	ration	899:904	arg1	silage					934:939	31% grass silage	924:939	31% grass silage	924:939	A total mixed ration (42% corn silage, 31% grass silage, and 27% concentrate on a dry matter basis) formulated to meet 100 and 83% of net energy and metabolizable protein requirements, respectively, was fed at 90% of ad libitum intake by individual cow.
31155242	4	105	dep	ration	899:904	arg1	silage					916:921	42% corn silage	907:921	42% corn silage	907:921	A total mixed ration (42% corn silage, 31% grass silage, and 27% concentrate on a dry matter basis) formulated to meet 100 and 83% of net energy and metabolizable protein requirements, respectively, was fed at 90% of ad libitum intake by individual cow.
31155242	1	106	theme	gland	166:170	arg1	metabolism					172:181	mammary gland metabolism	158:181	mammary gland metabolism in lactating dairy cattle	158:207	We investigated mammary gland metabolism in lactating dairy cattle in response to energy from glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates at low (LMP) and high (HMP) metabolizable protein levels.
31155242	8	107	theme	fatty	1638:1642	arg1	LCFA					1651:1654	LCFA	1651:1654	LCFA	1651:1654	Infusion of GG increased arterial plasma concentrations of glucose and insulin and decreased concentrations of β-hydroxybutyrate (BHB), nonesterified fatty acids, long-chain fatty acids (LCFA), total AA, EAA, and group 2 AA.
31155242	8	107	theme	fatty	1638:1642	arg1	acids					1644:1648	long-chain fatty acids	1627:1648	long-chain fatty acids (LCFA)	1627:1655	Infusion of GG increased arterial plasma concentrations of glucose and insulin and decreased concentrations of β-hydroxybutyrate (BHB), nonesterified fatty acids, long-chain fatty acids (LCFA), total AA, EAA, and group 2 AA.
31155242	13	108	theme	AA	2303:2304	arg1	group					2306:2310	any AA group	2299:2310	any AA group	2299:2310	Infusion of LG did not affect uptake or clearance of any AA group.
31155242	2	109	theme	g/d	616:618	arg1	LMP-LG					632:637	LMP-LG	632:637	LMP-LG	632:637	According to a 6 × 6 Latin square design, 6 rumen-fistulated second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were abomasally infused with saline (LMP-C); isoenergetic infusions (digestible energy basis) of 1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA (EAA; HMP-C); or isoenergetic infusions of 1,319 g/d glucose + 844 g/d EAA (HMP-GG) or 676 g/d palm olein + 844 g/d EAA (HMP-LG).
31155242	2	109	theme	g/d	616:618	arg1	olein					625:629	676 g/d palm olein	612:629	676 g/d palm olein (LMP-LG)	612:638	According to a 6 × 6 Latin square design, 6 rumen-fistulated second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were abomasally infused with saline (LMP-C); isoenergetic infusions (digestible energy basis) of 1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA (EAA; HMP-C); or isoenergetic infusions of 1,319 g/d glucose + 844 g/d EAA (HMP-GG) or 676 g/d palm olein + 844 g/d EAA (HMP-LG).
31155242	8	110	theme	GG	1476:1477	arg1	Infusion					1464:1471	Infusion	1464:1471	Infusion of GG	1464:1477	Infusion of GG increased arterial plasma concentrations of glucose and insulin and decreased concentrations of β-hydroxybutyrate (BHB), nonesterified fatty acids, long-chain fatty acids (LCFA), total AA, EAA, and group 2 AA.
31155242	7	111	theme	milk	1360:1363	arg1	protein					1365:1371	milk protein or lactose yield	1360:1388	protein	1365:1371	Neither GG nor LG infusion stimulated milk protein or lactose yield, but fat yield tended to decrease with GG and tended to increase with LG.
31155242	18	112	theme	estimated	2886:2894	arg1	requirements					2896:2907	estimated requirements	2886:2907	estimated requirements for lactose and fat synthesis	2886:2937	Mammary glucose uptake did not cover estimated requirements for lactose and fat synthesis at the HMP level, except during HMP-GG infusion.
31155242	4	113	theme	protein	1048:1054	arg1	requirements					1056:1067	metabolizable protein requirements	1034:1067	metabolizable protein requirements	1034:1067	A total mixed ration (42% corn silage, 31% grass silage, and 27% concentrate on a dry matter basis) formulated to meet 100 and 83% of net energy and metabolizable protein requirements, respectively, was fed at 90% of ad libitum intake by individual cow.
31155242	0	114	theme	metabolizable	112:124	arg1	levels					134:139	low and high metabolizable protein levels	99:139	low and high metabolizable protein levels	99:139	Mammary gland metabolite utilization in response to exogenous glucose or long-chain fatty acids at low and high metabolizable protein levels.
31155242	1	115	theme	dairy	196:200	arg1	cattle					202:207	lactating dairy cattle	186:207	lactating dairy cattle	186:207	We investigated mammary gland metabolism in lactating dairy cattle in response to energy from glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates at low (LMP) and high (HMP) metabolizable protein levels.
31155242	15	116	theme	BHB	2497:2499	arg1	uptake					2501:2506	BHB uptake	2497:2506	BHB uptake	2497:2506	Infusion of GG tended to increase mammary glucose uptake, and tended to decrease BHB uptake only at the LMP level.
31155242	2	117	theme	±	471:471	arg1	d					476:476	97 ± 13 d	468:476	97 ± 13 d in milk	468:484	According to a 6 × 6 Latin square design, 6 rumen-fistulated second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were abomasally infused with saline (LMP-C); isoenergetic infusions (digestible energy basis) of 1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA (EAA; HMP-C); or isoenergetic infusions of 1,319 g/d glucose + 844 g/d EAA (HMP-GG) or 676 g/d palm olein + 844 g/d EAA (HMP-LG).
31155242	6	118	theme	LMP	1311:1313	arg1	level					1315:1319	the LMP level	1307:1319	the LMP level	1307:1319	Infusing GG or LG at the HMP level did not affect milk yield or composition differently than at the LMP level.
31155242	18	119	theme	glucose	2857:2863	arg1	uptake					2865:2870	Mammary glucose uptake	2849:2870	Mammary glucose uptake	2849:2870	Mammary glucose uptake did not cover estimated requirements for lactose and fat synthesis at the HMP level, except during HMP-GG infusion.
31155242	6	120	from	level	1240:1244	arg1	GG					1220:1221	GG	1220:1221	GG	1220:1221	Infusing GG or LG at the HMP level did not affect milk yield or composition differently than at the LMP level.
31155242	6	120	from	level	1240:1244	arg1	LG					1226:1227	LG	1226:1227	LG	1226:1227	Infusing GG or LG at the HMP level did not affect milk yield or composition differently than at the LMP level.
31155242	4	121	theme	%	909:909	arg1	silage					916:921	42% corn silage	907:921	42% corn silage	907:921	A total mixed ration (42% corn silage, 31% grass silage, and 27% concentrate on a dry matter basis) formulated to meet 100 and 83% of net energy and metabolizable protein requirements, respectively, was fed at 90% of ad libitum intake by individual cow.
31155242	4	122	theme	net	1019:1021	arg1	energy					1023:1028	net energy	1019:1028	net energy	1019:1028	A total mixed ration (42% corn silage, 31% grass silage, and 27% concentrate on a dry matter basis) formulated to meet 100 and 83% of net energy and metabolizable protein requirements, respectively, was fed at 90% of ad libitum intake by individual cow.
31155242	8	123	theme	glucose	1523:1529	arg1	concentrations					1505:1518	arterial plasma concentrations	1489:1518	arterial plasma concentrations of glucose and insulin	1489:1541	Infusion of GG increased arterial plasma concentrations of glucose and insulin and decreased concentrations of β-hydroxybutyrate (BHB), nonesterified fatty acids, long-chain fatty acids (LCFA), total AA, EAA, and group 2 AA.
31155242	16	124	theme	LG	2543:2544	arg1	Infusion					2531:2538	Infusion	2531:2538	Infusion of LG	2531:2544	Infusion of LG increased mammary uptake of TAG and LCFA and increased or tended to increase clearance of TAG and LCFA.
31155242	8	125	theme	arterial	1489:1496	arg1	concentrations					1505:1518	arterial plasma concentrations	1489:1518	arterial plasma concentrations of glucose and insulin	1489:1541	Infusion of GG increased arterial plasma concentrations of glucose and insulin and decreased concentrations of β-hydroxybutyrate (BHB), nonesterified fatty acids, long-chain fatty acids (LCFA), total AA, EAA, and group 2 AA.
31155242	12	126	theme	GG	2129:2130	arg1	interactions					2137:2148	GG × AA interactions	2129:2148	GG × AA interactions	2129:2148	Uptake and clearance of total EAA and group 2 AA were affected or tended to be affected by GG × AA interactions, with their uptakes being lower and their clearances higher with GG, but only at the LMP level.
31155242	8	127	theme	insulin	1535:1541	arg1	concentrations					1505:1518	arterial plasma concentrations	1489:1518	arterial plasma concentrations of glucose and insulin	1489:1541	Infusion of GG increased arterial plasma concentrations of glucose and insulin and decreased concentrations of β-hydroxybutyrate (BHB), nonesterified fatty acids, long-chain fatty acids (LCFA), total AA, EAA, and group 2 AA.
31155242	1	128	theme	protein	344:350	arg1	HMP					325:327	HMP	325:327	HMP	325:327	We investigated mammary gland metabolism in lactating dairy cattle in response to energy from glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates at low (LMP) and high (HMP) metabolizable protein levels.
31155242	1	128	theme	protein	344:350	arg1	levels					352:357	low (LMP) and high (HMP) metabolizable protein levels	305:357	low (LMP) and high (HMP) metabolizable protein levels	305:357	We investigated mammary gland metabolism in lactating dairy cattle in response to energy from glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates at low (LMP) and high (HMP) metabolizable protein levels.
31155242	6	129	theme	Infusing	1211:1218	arg1	GG					1220:1221	GG	1220:1221	GG	1220:1221	Infusing GG or LG at the HMP level did not affect milk yield or composition differently than at the LMP level.
31155242	4	130	theme	mixed	893:897	arg1	ration					899:904	A total mixed ration	885:904	A total mixed ration (42% corn silage, 31% grass silage, and 27% concentrate on a dry matter basis)	885:983	A total mixed ration (42% corn silage, 31% grass silage, and 27% concentrate on a dry matter basis) formulated to meet 100 and 83% of net energy and metabolizable protein requirements, respectively, was fed at 90% of ad libitum intake by individual cow.
31155242	1	131	from	levels	352:357	arg1	energy					224:229	energy	224:229	energy from glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates at low (LMP) and high (HMP) metabolizable protein levels	224:357	We investigated mammary gland metabolism in lactating dairy cattle in response to energy from glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates at low (LMP) and high (HMP) metabolizable protein levels.
31155242	13	132	theme	LG	2258:2259	arg1	Infusion					2246:2253	Infusion	2246:2253	Infusion of LG	2246:2259	Infusion of LG did not affect uptake or clearance of any AA group.
31155242	19	133	theme	absorptive	3072:3081	arg1	supply					3083:3088	absorptive supply	3072:3088	absorptive supply of glucogenic, lipogenic, and aminogenic substrate	3072:3139	Results of this study illustrate flexibility in mammary metabolite utilization when absorptive supply of glucogenic, lipogenic, and aminogenic substrate is increased.
31155242	2	134	theme	g/d	756:758	arg1	olein					765:769	676 g/d palm olein	752:769	676 g/d palm olein	752:769	According to a 6 × 6 Latin square design, 6 rumen-fistulated second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were abomasally infused with saline (LMP-C); isoenergetic infusions (digestible energy basis) of 1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA (EAA; HMP-C); or isoenergetic infusions of 1,319 g/d glucose + 844 g/d EAA (HMP-GG) or 676 g/d palm olein + 844 g/d EAA (HMP-LG).
31155242	17	135	dep	GG	2661:2662	arg1	increased					2664:2672	increased	2664:2672	increased mammary plasma flow to maintain intramammary energy and AA balance	2664:2739	We suspect GG increased mammary plasma flow to maintain intramammary energy and AA balance and stimulated lipogenesis in adipose, accounting for depressed arterial BHB and group 2 AA concentrations.
31155242	17	135	dep	GG	2661:2662	arg1	stimulated					2745:2754	stimulated	2745:2754	stimulated lipogenesis in adipose	2745:2777	We suspect GG increased mammary plasma flow to maintain intramammary energy and AA balance and stimulated lipogenesis in adipose, accounting for depressed arterial BHB and group 2 AA concentrations.
31155242	0	136	theme	fatty	84:88	arg1	acids					90:94	long-chain fatty acids	73:94	long-chain fatty acids	73:94	Mammary gland metabolite utilization in response to exogenous glucose or long-chain fatty acids at low and high metabolizable protein levels.
31155242	1	137	from	metabolism	172:181	arg1	cattle					202:207	lactating dairy cattle	186:207	lactating dairy cattle	186:207	We investigated mammary gland metabolism in lactating dairy cattle in response to energy from glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates at low (LMP) and high (HMP) metabolizable protein levels.
31155242	10	138	theme	arterial	1848:1855	arg1	concentrations					1857:1870	arterial concentrations	1848:1870	arterial concentrations of BHB, urea, and all EAA groups	1848:1903	Compared with the LMP level, the HMP level increased arterial concentrations of BHB, urea, and all EAA groups and decreased the concentration of total non-EAA.
31155242	17	139	theme	AA	2730:2731	arg1	balance					2733:2739	intramammary energy and AA balance	2706:2739	balance	2733:2739	We suspect GG increased mammary plasma flow to maintain intramammary energy and AA balance and stimulated lipogenesis in adipose, accounting for depressed arterial BHB and group 2 AA concentrations.
31155242	9	140	theme	EAA	1775:1777	arg1	concentrations					1779:1792	EAA concentrations	1775:1792	EAA concentrations	1775:1792	Infusion of LG increased arterial triacylglycerides (TAG) and LCFA but did not affect EAA concentrations.
31155242	1	141	theme	low	305:307	arg1	HMP					325:327	HMP	325:327	HMP	325:327	We investigated mammary gland metabolism in lactating dairy cattle in response to energy from glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates at low (LMP) and high (HMP) metabolizable protein levels.
31155242	1	141	theme	low	305:307	arg1	levels					352:357	low (LMP) and high (HMP) metabolizable protein levels	305:357	low (LMP) and high (HMP) metabolizable protein levels	305:357	We investigated mammary gland metabolism in lactating dairy cattle in response to energy from glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates at low (LMP) and high (HMP) metabolizable protein levels.
31155242	16	142	theme	mammary	2556:2562	arg1	uptake					2564:2569	mammary uptake	2556:2569	mammary uptake of TAG and LCFA	2556:2585	Infusion of LG increased mammary uptake of TAG and LCFA and increased or tended to increase clearance of TAG and LCFA.
31155242	2	143	theme	dairy	456:460	arg1	cows					462:465	6 rumen-fistulated second-lactation Holstein-Friesian dairy cows	402:465	6 rumen-fistulated second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk)	402:485	According to a 6 × 6 Latin square design, 6 rumen-fistulated second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were abomasally infused with saline (LMP-C); isoenergetic infusions (digestible energy basis) of 1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA (EAA; HMP-C); or isoenergetic infusions of 1,319 g/d glucose + 844 g/d EAA (HMP-GG) or 676 g/d palm olein + 844 g/d EAA (HMP-LG).
31155242	2	144	theme	844	773:775	arg1	g/d					777:779	g/d	777:779	g/d	777:779	According to a 6 × 6 Latin square design, 6 rumen-fistulated second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were abomasally infused with saline (LMP-C); isoenergetic infusions (digestible energy basis) of 1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA (EAA; HMP-C); or isoenergetic infusions of 1,319 g/d glucose + 844 g/d EAA (HMP-GG) or 676 g/d palm olein + 844 g/d EAA (HMP-LG).
31155242	2	145	theme	isoenergetic	532:543	arg1	infusions					545:553	isoenergetic infusions	532:553	isoenergetic infusions (digestible energy basis) of 1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA (EAA; HMP-C)	532:676	According to a 6 × 6 Latin square design, 6 rumen-fistulated second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were abomasally infused with saline (LMP-C); isoenergetic infusions (digestible energy basis) of 1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA (EAA; HMP-C); or isoenergetic infusions of 1,319 g/d glucose + 844 g/d EAA (HMP-GG) or 676 g/d palm olein + 844 g/d EAA (HMP-LG).
31155242	2	145	theme	isoenergetic	532:543	arg1	basis					574:578	digestible energy basis	556:578	digestible energy basis	556:578	According to a 6 × 6 Latin square design, 6 rumen-fistulated second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were abomasally infused with saline (LMP-C); isoenergetic infusions (digestible energy basis) of 1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA (EAA; HMP-C); or isoenergetic infusions of 1,319 g/d glucose + 844 g/d EAA (HMP-GG) or 676 g/d palm olein + 844 g/d EAA (HMP-LG).
31155242	8	146	theme	plasma	1498:1503	arg1	concentrations					1505:1518	arterial plasma concentrations	1489:1518	arterial plasma concentrations of glucose and insulin	1489:1541	Infusion of GG increased arterial plasma concentrations of glucose and insulin and decreased concentrations of β-hydroxybutyrate (BHB), nonesterified fatty acids, long-chain fatty acids (LCFA), total AA, EAA, and group 2 AA.
31155242	15	147	theme	glucose	2458:2464	arg1	uptake					2466:2471	mammary glucose uptake	2450:2471	mammary glucose uptake	2450:2471	Infusion of GG tended to increase mammary glucose uptake, and tended to decrease BHB uptake only at the LMP level.
31155242	3	148	theme	continuous	840:849	arg1	infusion					851:858	continuous infusion	840:858	continuous infusion	840:858	Each experimental period consisted of 5 d of continuous infusion followed by 2 d of rest.
31155242	2	149	theme	second-lactation	421:436	arg1	cows					462:465	6 rumen-fistulated second-lactation Holstein-Friesian dairy cows	402:465	6 rumen-fistulated second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk)	402:485	According to a 6 × 6 Latin square design, 6 rumen-fistulated second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were abomasally infused with saline (LMP-C); isoenergetic infusions (digestible energy basis) of 1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA (EAA; HMP-C); or isoenergetic infusions of 1,319 g/d glucose + 844 g/d EAA (HMP-GG) or 676 g/d palm olein + 844 g/d EAA (HMP-LG).
31155242	4	150	theme	31	924:925	arg1	%					926:926	%	926:926	%	926:926	A total mixed ration (42% corn silage, 31% grass silage, and 27% concentrate on a dry matter basis) formulated to meet 100 and 83% of net energy and metabolizable protein requirements, respectively, was fed at 90% of ad libitum intake by individual cow.
31155242	0	151	theme	gland	8:12	arg1	utilization					25:35	Mammary gland metabolite utilization	0:35	Mammary gland metabolite utilization in response to exogenous glucose or long-chain fatty acids at low and high metabolizable protein levels.	0:140	Mammary gland metabolite utilization in response to exogenous glucose or long-chain fatty acids at low and high metabolizable protein levels.
31155242	6	152	theme	HMP	1236:1238	arg1	level					1240:1244	the HMP level	1232:1244	the HMP level	1232:1244	Infusing GG or LG at the HMP level did not affect milk yield or composition differently than at the LMP level.
31155242	10	153	theme	total	1940:1944	arg1	non-EAA					1946:1952	total non-EAA	1940:1952	total non-EAA	1940:1952	Compared with the LMP level, the HMP level increased arterial concentrations of BHB, urea, and all EAA groups and decreased the concentration of total non-EAA.
31155242	2	154	theme	energy	567:572	arg1	infusions					545:553	isoenergetic infusions	532:553	isoenergetic infusions (digestible energy basis) of 1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA (EAA; HMP-C)	532:676	According to a 6 × 6 Latin square design, 6 rumen-fistulated second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were abomasally infused with saline (LMP-C); isoenergetic infusions (digestible energy basis) of 1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA (EAA; HMP-C); or isoenergetic infusions of 1,319 g/d glucose + 844 g/d EAA (HMP-GG) or 676 g/d palm olein + 844 g/d EAA (HMP-LG).
31155242	2	154	theme	energy	567:572	arg1	basis					574:578	digestible energy basis	556:578	digestible energy basis	556:578	According to a 6 × 6 Latin square design, 6 rumen-fistulated second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were abomasally infused with saline (LMP-C); isoenergetic infusions (digestible energy basis) of 1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA (EAA; HMP-C); or isoenergetic infusions of 1,319 g/d glucose + 844 g/d EAA (HMP-GG) or 676 g/d palm olein + 844 g/d EAA (HMP-LG).
31155242	8	155	theme	acids	1620:1624	arg1	concentrations					1557:1570	concentrations	1557:1570	concentrations of β-hydroxybutyrate (BHB), nonesterified fatty acids, long-chain fatty acids (LCFA), total AA, EAA, and group 2 AA	1557:1686	Infusion of GG increased arterial plasma concentrations of glucose and insulin and decreased concentrations of β-hydroxybutyrate (BHB), nonesterified fatty acids, long-chain fatty acids (LCFA), total AA, EAA, and group 2 AA.
31155242	18	156	theme	HMP	2946:2948	arg1	level					2950:2954	the HMP level	2942:2954	the HMP level	2942:2954	Mammary glucose uptake did not cover estimated requirements for lactose and fat synthesis at the HMP level, except during HMP-GG infusion.
31155242	8	157	theme	AA	1664:1665	arg1	concentrations					1557:1570	concentrations	1557:1570	concentrations of β-hydroxybutyrate (BHB), nonesterified fatty acids, long-chain fatty acids (LCFA), total AA, EAA, and group 2 AA	1557:1686	Infusion of GG increased arterial plasma concentrations of glucose and insulin and decreased concentrations of β-hydroxybutyrate (BHB), nonesterified fatty acids, long-chain fatty acids (LCFA), total AA, EAA, and group 2 AA.
31155242	2	158	theme	glucose	718:724	arg1	HMP-GG					741:746	HMP-GG	741:746	HMP-GG	741:746	According to a 6 × 6 Latin square design, 6 rumen-fistulated second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were abomasally infused with saline (LMP-C); isoenergetic infusions (digestible energy basis) of 1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA (EAA; HMP-C); or isoenergetic infusions of 1,319 g/d glucose + 844 g/d EAA (HMP-GG) or 676 g/d palm olein + 844 g/d EAA (HMP-LG).
31155242	2	158	theme	glucose	718:724	arg1	infusions					545:553	isoenergetic infusions	532:553	isoenergetic infusions (digestible energy basis) of 1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA (EAA; HMP-C)	532:676	According to a 6 × 6 Latin square design, 6 rumen-fistulated second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were abomasally infused with saline (LMP-C); isoenergetic infusions (digestible energy basis) of 1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA (EAA; HMP-C); or isoenergetic infusions of 1,319 g/d glucose + 844 g/d EAA (HMP-GG) or 676 g/d palm olein + 844 g/d EAA (HMP-LG).
31155242	2	158	theme	glucose	718:724	arg1	saline					516:521	saline	516:521	saline (LMP-C)	516:529	According to a 6 × 6 Latin square design, 6 rumen-fistulated second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were abomasally infused with saline (LMP-C); isoenergetic infusions (digestible energy basis) of 1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA (EAA; HMP-C); or isoenergetic infusions of 1,319 g/d glucose + 844 g/d EAA (HMP-GG) or 676 g/d palm olein + 844 g/d EAA (HMP-LG).
31155242	2	158	theme	glucose	718:724	arg1	LMP-C					524:528	LMP-C	524:528	LMP-C	524:528	According to a 6 × 6 Latin square design, 6 rumen-fistulated second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were abomasally infused with saline (LMP-C); isoenergetic infusions (digestible energy basis) of 1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA (EAA; HMP-C); or isoenergetic infusions of 1,319 g/d glucose + 844 g/d EAA (HMP-GG) or 676 g/d palm olein + 844 g/d EAA (HMP-LG).
31155242	2	158	theme	glucose	718:724	arg1	infusions					695:703	isoenergetic infusions	682:703	isoenergetic infusions of 1,319 g/d glucose	682:724	According to a 6 × 6 Latin square design, 6 rumen-fistulated second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were abomasally infused with saline (LMP-C); isoenergetic infusions (digestible energy basis) of 1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA (EAA; HMP-C); or isoenergetic infusions of 1,319 g/d glucose + 844 g/d EAA (HMP-GG) or 676 g/d palm olein + 844 g/d EAA (HMP-LG).
31155242	2	158	theme	glucose	718:724	arg1	basis					574:578	digestible energy basis	556:578	digestible energy basis	556:578	According to a 6 × 6 Latin square design, 6 rumen-fistulated second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were abomasally infused with saline (LMP-C); isoenergetic infusions (digestible energy basis) of 1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA (EAA; HMP-C); or isoenergetic infusions of 1,319 g/d glucose + 844 g/d EAA (HMP-GG) or 676 g/d palm olein + 844 g/d EAA (HMP-LG).
31155242	4	159	theme	matter	971:976	arg1	basis					978:982	a dry matter basis	965:982	a dry matter basis	965:982	A total mixed ration (42% corn silage, 31% grass silage, and 27% concentrate on a dry matter basis) formulated to meet 100 and 83% of net energy and metabolizable protein requirements, respectively, was fed at 90% of ad libitum intake by individual cow.
31155242	17	160	theme	mammary	2674:2680	arg1	flow					2689:2692	mammary plasma flow	2674:2692	mammary plasma flow to maintain intramammary energy and AA balance	2674:2739	We suspect GG increased mammary plasma flow to maintain intramammary energy and AA balance and stimulated lipogenesis in adipose, accounting for depressed arterial BHB and group 2 AA concentrations.
31155242	4	161	theme	libitum	1105:1111	arg1	intake					1113:1118	ad libitum intake	1102:1118	ad libitum intake	1102:1118	A total mixed ration (42% corn silage, 31% grass silage, and 27% concentrate on a dry matter basis) formulated to meet 100 and 83% of net energy and metabolizable protein requirements, respectively, was fed at 90% of ad libitum intake by individual cow.
31155242	8	162	theme	long-chain	1627:1636	arg1	LCFA					1651:1654	LCFA	1651:1654	LCFA	1651:1654	Infusion of GG increased arterial plasma concentrations of glucose and insulin and decreased concentrations of β-hydroxybutyrate (BHB), nonesterified fatty acids, long-chain fatty acids (LCFA), total AA, EAA, and group 2 AA.
31155242	8	162	theme	long-chain	1627:1636	arg1	acids					1644:1648	long-chain fatty acids	1627:1648	long-chain fatty acids (LCFA)	1627:1655	Infusion of GG increased arterial plasma concentrations of glucose and insulin and decreased concentrations of β-hydroxybutyrate (BHB), nonesterified fatty acids, long-chain fatty acids (LCFA), total AA, EAA, and group 2 AA.
31155242	2	163	dep	cows	462:465	arg1	d					476:476	97 ± 13 d	468:476	97 ± 13 d in milk	468:484	According to a 6 × 6 Latin square design, 6 rumen-fistulated second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were abomasally infused with saline (LMP-C); isoenergetic infusions (digestible energy basis) of 1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA (EAA; HMP-C); or isoenergetic infusions of 1,319 g/d glucose + 844 g/d EAA (HMP-GG) or 676 g/d palm olein + 844 g/d EAA (HMP-LG).
31155242	8	164	theme	EAA	1668:1670	arg1	concentrations					1557:1570	concentrations	1557:1570	concentrations of β-hydroxybutyrate (BHB), nonesterified fatty acids, long-chain fatty acids (LCFA), total AA, EAA, and group 2 AA	1557:1686	Infusion of GG increased arterial plasma concentrations of glucose and insulin and decreased concentrations of β-hydroxybutyrate (BHB), nonesterified fatty acids, long-chain fatty acids (LCFA), total AA, EAA, and group 2 AA.
31155242	2	165	theme	Latin	381:385	arg1	design					394:399	a 6 × 6 Latin square design	373:399	a 6 × 6 Latin square design	373:399	According to a 6 × 6 Latin square design, 6 rumen-fistulated second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were abomasally infused with saline (LMP-C); isoenergetic infusions (digestible energy basis) of 1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA (EAA; HMP-C); or isoenergetic infusions of 1,319 g/d glucose + 844 g/d EAA (HMP-GG) or 676 g/d palm olein + 844 g/d EAA (HMP-LG).
31155242	17	166	theme	depressed	2795:2803	arg1	BHB					2814:2816	depressed arterial BHB	2795:2816	depressed arterial BHB	2795:2816	We suspect GG increased mammary plasma flow to maintain intramammary energy and AA balance and stimulated lipogenesis in adipose, accounting for depressed arterial BHB and group 2 AA concentrations.
31155242	10	167	theme	groups	1898:1903	arg1	concentrations					1857:1870	arterial concentrations	1848:1870	arterial concentrations of BHB, urea, and all EAA groups	1848:1903	Compared with the LMP level, the HMP level increased arterial concentrations of BHB, urea, and all EAA groups and decreased the concentration of total non-EAA.
31155242	0	168	theme	exogenous	52:60	arg1	glucose					62:68	exogenous glucose	52:68	exogenous glucose	52:68	Mammary gland metabolite utilization in response to exogenous glucose or long-chain fatty acids at low and high metabolizable protein levels.
31155242	2	169	theme	×	377:377	arg1	design					394:399	a 6 × 6 Latin square design	373:399	a 6 × 6 Latin square design	373:399	According to a 6 × 6 Latin square design, 6 rumen-fistulated second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were abomasally infused with saline (LMP-C); isoenergetic infusions (digestible energy basis) of 1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA (EAA; HMP-C); or isoenergetic infusions of 1,319 g/d glucose + 844 g/d EAA (HMP-GG) or 676 g/d palm olein + 844 g/d EAA (HMP-LG).
31155242	5	170	theme	blood	1159:1163	arg1	samples					1165:1171	Arterial and venous blood samples	1139:1171	Arterial and venous blood samples	1139:1171	Arterial and venous blood samples were collected on d 5 of each period.
31155242	19	171	theme	study	3004:3008	arg1	Results					2988:2994	Results	2988:2994	Results of this study	2988:3008	Results of this study illustrate flexibility in mammary metabolite utilization when absorptive supply of glucogenic, lipogenic, and aminogenic substrate is increased.
31155242	12	172	theme	total	2062:2066	arg1	EAA					2068:2070	total EAA and group 2 AA	2062:2085	EAA	2068:2070	Uptake and clearance of total EAA and group 2 AA were affected or tended to be affected by GG × AA interactions, with their uptakes being lower and their clearances higher with GG, but only at the LMP level.
31155242	15	173	theme	mammary	2450:2456	arg1	uptake					2466:2471	mammary glucose uptake	2450:2471	mammary glucose uptake	2450:2471	Infusion of GG tended to increase mammary glucose uptake, and tended to decrease BHB uptake only at the LMP level.
31155242	2	174	theme	g/d	648:650	arg1	EAA					666:668	EAA	666:668	EAA	666:668	According to a 6 × 6 Latin square design, 6 rumen-fistulated second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were abomasally infused with saline (LMP-C); isoenergetic infusions (digestible energy basis) of 1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA (EAA; HMP-C); or isoenergetic infusions of 1,319 g/d glucose + 844 g/d EAA (HMP-GG) or 676 g/d palm olein + 844 g/d EAA (HMP-LG).
31155242	2	174	theme	g/d	648:650	arg1	AA					662:663	1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA	584:663	1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA (EAA; HMP-C)	584:676	According to a 6 × 6 Latin square design, 6 rumen-fistulated second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were abomasally infused with saline (LMP-C); isoenergetic infusions (digestible energy basis) of 1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA (EAA; HMP-C); or isoenergetic infusions of 1,319 g/d glucose + 844 g/d EAA (HMP-GG) or 676 g/d palm olein + 844 g/d EAA (HMP-LG).
31155242	0	175	from	levels	134:139	arg1	utilization					25:35	Mammary gland metabolite utilization	0:35	Mammary gland metabolite utilization in response to exogenous glucose or long-chain fatty acids at low and high metabolizable protein levels.	0:140	Mammary gland metabolite utilization in response to exogenous glucose or long-chain fatty acids at low and high metabolizable protein levels.
31155242	18	176	theme	HMP-GG	2971:2976	arg1	infusion					2978:2985	HMP-GG infusion	2971:2985	HMP-GG infusion	2971:2985	Mammary glucose uptake did not cover estimated requirements for lactose and fat synthesis at the HMP level, except during HMP-GG infusion.
31155242	2	177	theme	glucose	594:600	arg1	EAA					666:668	EAA	666:668	EAA	666:668	According to a 6 × 6 Latin square design, 6 rumen-fistulated second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were abomasally infused with saline (LMP-C); isoenergetic infusions (digestible energy basis) of 1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA (EAA; HMP-C); or isoenergetic infusions of 1,319 g/d glucose + 844 g/d EAA (HMP-GG) or 676 g/d palm olein + 844 g/d EAA (HMP-LG).
31155242	2	177	theme	glucose	594:600	arg1	AA					662:663	1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA	584:663	1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA (EAA; HMP-C)	584:676	According to a 6 × 6 Latin square design, 6 rumen-fistulated second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were abomasally infused with saline (LMP-C); isoenergetic infusions (digestible energy basis) of 1,319 g/d glucose (LMP-GG), 676 g/d palm olein (LMP-LG), or 844 g/d essential AA (EAA; HMP-C); or isoenergetic infusions of 1,319 g/d glucose + 844 g/d EAA (HMP-GG) or 676 g/d palm olein + 844 g/d EAA (HMP-LG).
31155242	19	178	theme	mammary	3036:3042	arg1	utilization					3055:3065	mammary metabolite utilization	3036:3065	mammary metabolite utilization	3036:3065	Results of this study illustrate flexibility in mammary metabolite utilization when absorptive supply of glucogenic, lipogenic, and aminogenic substrate is increased.
31155242	11	179	theme	plasma	1963:1968	arg1	flow					1970:1973	Mammary plasma flow	1955:1973	Mammary plasma flow	1955:1973	Mammary plasma flow increased with GG and was not affected by LG or protein level.
31155242	17	180	theme	AA	2830:2831	arg1	concentrations					2833:2846	AA concentrations	2830:2846	AA concentrations	2830:2846	We suspect GG increased mammary plasma flow to maintain intramammary energy and AA balance and stimulated lipogenesis in adipose, accounting for depressed arterial BHB and group 2 AA concentrations.
31155242	8	181	theme	nonesterified	1600:1612	arg1	acids					1620:1624	nonesterified fatty acids	1600:1624	nonesterified fatty acids	1600:1624	Infusion of GG increased arterial plasma concentrations of glucose and insulin and decreased concentrations of β-hydroxybutyrate (BHB), nonesterified fatty acids, long-chain fatty acids (LCFA), total AA, EAA, and group 2 AA.
31155242	1	182	theme	glucogenic	236:245	arg1	substrates					291:300	glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates	236:300	glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates	236:300	We investigated mammary gland metabolism in lactating dairy cattle in response to energy from glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates at low (LMP) and high (HMP) metabolizable protein levels.
31810119	13	0	from	changes	2220:2226	arg1	expression					2231:2240	expression	2231:2240	expression of major Bcl-2 family effector proteins of the mitochondrial apoptosis pathway	2231:2319	Additionally, FLJC protected against H2O2-induced apoptosis by inhibiting changes in expression of major Bcl-2 family effector proteins of the mitochondrial apoptosis pathway.
31810119	6	1	theme	mesenchymal	899:909	arg1	differentiation					921:935	rat bone marrow mesenchymal stem cell differentiation	883:935	rat bone marrow mesenchymal stem cell differentiation induction	883:945	After rat bone marrow mesenchymal stem cell differentiation induction, resulting osteoblasts received various 48 h FLJC pretreatments before H2O2-based (200 μM) oxidative stress exposure.
31810119	2	2	theme	known	372:376	arg1	effect					396:401	a known anti-osteoporotic effect	370:401	a known anti-osteoporotic effect	370:401	Fufang Lurong Jiangu Capsule (FLJC) has a known anti-osteoporotic effect, but its pharmacological effect on osteoblasts is not clearly understood.
31810119	13	3	theme	proteins	2273:2280	arg1	expression					2231:2240	expression	2231:2240	expression of major Bcl-2 family effector proteins of the mitochondrial apoptosis pathway	2231:2319	Additionally, FLJC protected against H2O2-induced apoptosis by inhibiting changes in expression of major Bcl-2 family effector proteins of the mitochondrial apoptosis pathway.
31810119	16	4	theme	oxidative	2606:2614	arg1	damage					2616:2621	oxidative damage	2606:2621	oxidative damage of osteoblasts induced by H2O2 via the Nrf2/HO-1 signaling pathway	2606:2688	CONCLUSION These results indicate that FLJC may significantly attenuate oxidative damage of osteoblasts induced by H2O2 via the Nrf2/HO-1 signaling pathway, providing new insights to guide development of treatments for osteoporosis induced by oxidative injury.
31810119	3	5	from	mice	665:668	arg1	damage					595:600	in vitro hydrogen peroxide (H2O2)-induced oxidative damage	543:600	in vitro hydrogen peroxide (H2O2)-induced oxidative damage of osteoblasts	543:615	This study was designed to investigate FLJC effects/mechanisms on in vitro hydrogen peroxide (H2O2)-induced oxidative damage of osteoblasts and on in vivo lipopolysaccharide (LPS)-induced mice bone loss.
31810119	7	6	theme	mitochondria	1216:1227	arg1	mineralization					1294:1307	mineralization	1294:1307	mineralization	1294:1307	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	7	6	theme	mitochondria	1216:1227	arg1	levels					1145:1150	levels	1145:1150	levels of intracellular reactive oxygen species (ROS)	1145:1197	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	7	6	theme	mitochondria	1216:1227	arg1	activity					1230:1237	activity	1230:1237	activity of antioxidant enzymes	1230:1260	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	7	6	theme	mitochondria	1216:1227	arg1	expression					1337:1346	expression	1337:1346	expression of osteogenic markers	1337:1368	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	7	6	theme	mitochondria	1216:1227	arg1	phosphatase					1272:1282	alkaline phosphatase	1263:1282	alkaline phosphatase (ALP)	1263:1288	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	7	6	theme	mitochondria	1216:1227	arg1	viability					1111:1119	osteoblast cell viability	1095:1119	osteoblast cell viability	1095:1119	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	7	6	theme	mitochondria	1216:1227	arg1	ALP					1285:1287	ALP	1285:1287	ALP	1285:1287	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	7	6	theme	mitochondria	1216:1227	arg1	secretion					1314:1322	the secretion	1310:1322	the secretion of Col I	1310:1331	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	7	6	theme	mitochondria	1216:1227	arg1	localization					1200:1211	localization	1200:1211	localization of mitochondria	1200:1227	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	6	7	theme	bone	887:890	arg1	marrow					892:897	rat bone marrow	883:897	rat bone marrow mesenchymal stem cell differentiation induction	883:945	After rat bone marrow mesenchymal stem cell differentiation induction, resulting osteoblasts received various 48 h FLJC pretreatments before H2O2-based (200 μM) oxidative stress exposure.
31810119	3	8	theme	-induced	576:583	arg1	damage					595:600	in vitro hydrogen peroxide (H2O2)-induced oxidative damage	543:600	in vitro hydrogen peroxide (H2O2)-induced oxidative damage of osteoblasts	543:615	This study was designed to investigate FLJC effects/mechanisms on in vitro hydrogen peroxide (H2O2)-induced oxidative damage of osteoblasts and on in vivo lipopolysaccharide (LPS)-induced mice bone loss.
31810119	12	9	theme	mineralization	2107:2120	arg1	levels					2097:2102	levels	2097:2102	levels of mineralization and osteoblast markers	2097:2143	With regard to osteoblast differentiation, FLJC pretreatment increased ALP expression, as well as levels of mineralization and osteoblast markers.
31810119	12	9	theme	mineralization	2107:2120	arg1	expression					2074:2083	ALP expression	2070:2083	ALP expression	2070:2083	With regard to osteoblast differentiation, FLJC pretreatment increased ALP expression, as well as levels of mineralization and osteoblast markers.
31810119	6	10	theme	FLJC	992:995	arg1	pretreatments					997:1009	various 48 h FLJC pretreatments	979:1009	various 48 h FLJC pretreatments	979:1009	After rat bone marrow mesenchymal stem cell differentiation induction, resulting osteoblasts received various 48 h FLJC pretreatments before H2O2-based (200 μM) oxidative stress exposure.
31810119	9	11	theme	bone	1725:1728	arg1	loss					1730:1733	LPS-induced bone loss	1713:1733	LPS-induced bone loss	1713:1733	Hematoxylin and eosin (H&E) and ALP staining revealed in vivo FLJC effect on mice LPS-induced bone loss.
31810119	10	12	from	components	1758:1767	arg1	FLJC					1772:1775	FLJC	1772:1775	FLJC	1772:1775	RESULTS Five chemical components in FLJC were identified, and fingerprint analysis showed good reproducibility.
31810119	3	13	from	loss	675:678	arg1	damage					595:600	in vitro hydrogen peroxide (H2O2)-induced oxidative damage	543:600	in vitro hydrogen peroxide (H2O2)-induced oxidative damage of osteoblasts	543:615	This study was designed to investigate FLJC effects/mechanisms on in vitro hydrogen peroxide (H2O2)-induced oxidative damage of osteoblasts and on in vivo lipopolysaccharide (LPS)-induced mice bone loss.
31810119	1	14	theme	Oxidative	204:212	arg1	stress					214:219	OBJECTIVE Oxidative stress	194:219	OBJECTIVE Oxidative stress	194:219	OBJECTIVE Oxidative stress is increasingly recognized as a risk factor associated with the development and progression of osteoporosis.
31810119	1	14	theme	Oxidative	204:212	arg1	factor					258:263	a risk factor	251:263	a risk factor associated with the development and progression of osteoporosis	251:327	OBJECTIVE Oxidative stress is increasingly recognized as a risk factor associated with the development and progression of osteoporosis.
31810119	6	15	theme	various	979:985	arg1	pretreatments					997:1009	various 48 h FLJC pretreatments	979:1009	various 48 h FLJC pretreatments	979:1009	After rat bone marrow mesenchymal stem cell differentiation induction, resulting osteoblasts received various 48 h FLJC pretreatments before H2O2-based (200 μM) oxidative stress exposure.
31810119	0	16	theme	Nrf2/HO-1	165:173	arg1	pathway					185:191	the Nrf2/HO-1 signaling pathway	161:191	the Nrf2/HO-1 signaling pathway	161:191	Cytoprotective effect of Fufang Lurong Jiangu capsule against hydrogen peroxide-induced oxidative stress in bone marrow stromal cell-derived osteoblasts through the Nrf2/HO-1 signaling pathway.
31810119	3	17	theme	osteoblasts	605:615	arg1	damage					595:600	in vitro hydrogen peroxide (H2O2)-induced oxidative damage	543:600	in vitro hydrogen peroxide (H2O2)-induced oxidative damage of osteoblasts	543:615	This study was designed to investigate FLJC effects/mechanisms on in vitro hydrogen peroxide (H2O2)-induced oxidative damage of osteoblasts and on in vivo lipopolysaccharide (LPS)-induced mice bone loss.
31810119	5	18	theme	METHODS	750:756	arg1	compositions					767:778	METHODS Chemical compositions	750:778	METHODS Chemical compositions of FLJC preparations	750:799	METHODS Chemical compositions of FLJC preparations were analyzed using high-performance liquid chromatographic fingerprinting.
31810119	15	19	theme	LPS-induced	2516:2526	arg1	mice					2528:2531	LPS-induced mice	2516:2531	LPS-induced mice	2516:2531	Finally, we confirmed that FLJC administration could reverse the bone loss in LPS-induced mice.
31810119	2	20	theme	Lurong	337:342	arg1	FLJC					360:363	FLJC	360:363	FLJC	360:363	Fufang Lurong Jiangu Capsule (FLJC) has a known anti-osteoporotic effect, but its pharmacological effect on osteoblasts is not clearly understood.
31810119	2	20	theme	Lurong	337:342	arg1	Capsule					351:357	Fufang Lurong Jiangu Capsule	330:357	Fufang Lurong Jiangu Capsule (FLJC)	330:364	Fufang Lurong Jiangu Capsule (FLJC) has a known anti-osteoporotic effect, but its pharmacological effect on osteoblasts is not clearly understood.
31810119	15	21	theme	FLJC	2465:2468	arg1	administration					2470:2483	FLJC administration	2465:2483	FLJC administration	2465:2483	Finally, we confirmed that FLJC administration could reverse the bone loss in LPS-induced mice.
31810119	11	22	theme	oxidative	1981:1989	arg1	damage					1991:1996	oxidative damage	1981:1996	oxidative damage	1981:1996	FLJC pretreatment significantly reduced H2O2-induced ROS levels in osteoblasts and increased antioxidant enzyme activities to reduce oxidative damage.
31810119	12	23	dep	differentiation	2025:2039	arg1	regard					2004:2009	regard	2004:2009	regard	2004:2009	With regard to osteoblast differentiation, FLJC pretreatment increased ALP expression, as well as levels of mineralization and osteoblast markers.
31810119	7	24	theme	oxygen	1178:1183	arg1	ROS					1194:1196	ROS	1194:1196	ROS	1194:1196	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	7	24	theme	oxygen	1178:1183	arg1	species					1185:1191	intracellular reactive oxygen species	1155:1191	intracellular reactive oxygen species (ROS)	1155:1197	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	13	25	theme	Bcl-2	2251:2255	arg1	proteins					2273:2280	major Bcl-2 family effector proteins	2245:2280	major Bcl-2 family effector proteins of the mitochondrial apoptosis pathway	2245:2319	Additionally, FLJC protected against H2O2-induced apoptosis by inhibiting changes in expression of major Bcl-2 family effector proteins of the mitochondrial apoptosis pathway.
31810119	7	26	theme	alkaline	1263:1270	arg1	phosphatase					1272:1282	alkaline phosphatase	1263:1282	alkaline phosphatase (ALP)	1263:1288	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	7	26	theme	alkaline	1263:1270	arg1	ALP					1285:1287	ALP	1285:1287	ALP	1285:1287	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	7	27	theme	morphological	1122:1134	arg1	viability					1111:1119	osteoblast cell viability	1095:1119	osteoblast cell viability	1095:1119	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	7	27	theme	morphological	1122:1134	arg1	changes					1136:1142	morphological changes	1122:1142	morphological changes	1122:1142	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	12	28	theme	ALP	2070:2072	arg1	expression					2074:2083	ALP expression	2070:2083	ALP expression	2070:2083	With regard to osteoblast differentiation, FLJC pretreatment increased ALP expression, as well as levels of mineralization and osteoblast markers.
31810119	6	29	theme	stress	1048:1053	arg1	exposure					1055:1062	H2O2-based (200 μM) oxidative stress exposure	1018:1062	H2O2-based (200 μM) oxidative stress exposure	1018:1062	After rat bone marrow mesenchymal stem cell differentiation induction, resulting osteoblasts received various 48 h FLJC pretreatments before H2O2-based (200 μM) oxidative stress exposure.
31810119	11	30	theme	ROS	1901:1903	arg1	levels					1905:1910	H2O2-induced ROS levels	1888:1910	H2O2-induced ROS levels in osteoblasts and increased antioxidant enzyme activities	1888:1969	FLJC pretreatment significantly reduced H2O2-induced ROS levels in osteoblasts and increased antioxidant enzyme activities to reduce oxidative damage.
31810119	7	31	theme	intracellular	1155:1167	arg1	ROS					1194:1196	ROS	1194:1196	ROS	1194:1196	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	7	31	theme	intracellular	1155:1167	arg1	species					1185:1191	intracellular reactive oxygen species	1155:1191	intracellular reactive oxygen species (ROS)	1155:1197	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	7	32	theme	osteoblast	1095:1104	arg1	levels					1145:1150	levels	1145:1150	levels of intracellular reactive oxygen species (ROS)	1145:1197	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	7	32	theme	osteoblast	1095:1104	arg1	activity					1230:1237	activity	1230:1237	activity of antioxidant enzymes	1230:1260	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	7	32	theme	osteoblast	1095:1104	arg1	expression					1337:1346	expression	1337:1346	expression of osteogenic markers	1337:1368	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	7	32	theme	osteoblast	1095:1104	arg1	viability					1111:1119	osteoblast cell viability	1095:1119	osteoblast cell viability	1095:1119	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	7	32	theme	osteoblast	1095:1104	arg1	secretion					1314:1322	the secretion	1310:1322	the secretion of Col I	1310:1331	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	7	32	theme	osteoblast	1095:1104	arg1	localization					1200:1211	localization	1200:1211	localization of mitochondria	1200:1227	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	7	32	theme	osteoblast	1095:1104	arg1	changes					1136:1142	morphological changes	1122:1142	morphological changes	1122:1142	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	16	33	theme	new	2701:2703	arg1	insights					2705:2712	new insights	2701:2712	new insights	2701:2712	CONCLUSION These results indicate that FLJC may significantly attenuate oxidative damage of osteoblasts induced by H2O2 via the Nrf2/HO-1 signaling pathway, providing new insights to guide development of treatments for osteoporosis induced by oxidative injury.
31810119	1	34	dep	development	285:295	arg1	the					281:283	the	281:283	the	281:283	OBJECTIVE Oxidative stress is increasingly recognized as a risk factor associated with the development and progression of osteoporosis.
31810119	4	35	theme	unknown	714:720	arg1	mechanisms					738:747	unknown pharmacological mechanisms	714:747	unknown pharmacological mechanisms	714:747	FLJC alleviates osteoporosis via unknown pharmacological mechanisms.
31810119	7	36	theme	Col	1327:1329	arg1	mineralization					1294:1307	mineralization	1294:1307	mineralization	1294:1307	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	7	36	theme	Col	1327:1329	arg1	levels					1145:1150	levels	1145:1150	levels of intracellular reactive oxygen species (ROS)	1145:1197	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	7	36	theme	Col	1327:1329	arg1	activity					1230:1237	activity	1230:1237	activity of antioxidant enzymes	1230:1260	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	7	36	theme	Col	1327:1329	arg1	expression					1337:1346	expression	1337:1346	expression of osteogenic markers	1337:1368	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	7	36	theme	Col	1327:1329	arg1	phosphatase					1272:1282	alkaline phosphatase	1263:1282	alkaline phosphatase (ALP)	1263:1288	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	7	36	theme	Col	1327:1329	arg1	viability					1111:1119	osteoblast cell viability	1095:1119	osteoblast cell viability	1095:1119	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	7	36	theme	Col	1327:1329	arg1	ALP					1285:1287	ALP	1285:1287	ALP	1285:1287	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	7	36	theme	Col	1327:1329	arg1	secretion					1314:1322	the secretion	1310:1322	the secretion of Col I	1310:1331	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	7	36	theme	Col	1327:1329	arg1	localization					1200:1211	localization	1200:1211	localization of mitochondria	1200:1227	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	16	37	dep	CONCLUSION	2534:2543	arg1	indicate					2559:2566	indicate	2559:2566	indicate that FLJC may significantly attenuate oxidative damage of osteoblasts induced by H2O2 via the Nrf2/HO-1 signaling pathway, providing new insights to guide development of treatments for osteoporosis induced by oxidative injury	2559:2792	CONCLUSION These results indicate that FLJC may significantly attenuate oxidative damage of osteoblasts induced by H2O2 via the Nrf2/HO-1 signaling pathway, providing new insights to guide development of treatments for osteoporosis induced by oxidative injury.
31810119	8	38	theme	cytometric	1424:1433	arg1	analysis					1435:1442	flow cytometric analysis	1419:1442	flow cytometric analysis	1419:1442	The percentages of apoptosis were determined by flow cytometric analysis; apoptosis-related protein levels, including nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and heme oxygenase-1 (HO-1) with or without Nrf2 inhibitor were analyzed via western blot.
31810119	0	39	theme	oxidative	88:96	arg1	stress					98:103	hydrogen peroxide-induced oxidative stress	62:103	hydrogen peroxide-induced oxidative stress in bone marrow stromal cell-derived osteoblasts through the Nrf2/HO-1 signaling pathway	62:191	Cytoprotective effect of Fufang Lurong Jiangu capsule against hydrogen peroxide-induced oxidative stress in bone marrow stromal cell-derived osteoblasts through the Nrf2/HO-1 signaling pathway.
31810119	2	40	contain	has	366:368	arg1	FLJC					360:363	FLJC	360:363	FLJC	360:363	Fufang Lurong Jiangu Capsule (FLJC) has a known anti-osteoporotic effect, but its pharmacological effect on osteoblasts is not clearly understood.
31810119	2	40	contain	has	366:368	arg2	effect					396:401	a known anti-osteoporotic effect	370:401	a known anti-osteoporotic effect	370:401	Fufang Lurong Jiangu Capsule (FLJC) has a known anti-osteoporotic effect, but its pharmacological effect on osteoblasts is not clearly understood.
31810119	2	40	contain	has	366:368	arg1	Capsule					351:357	Fufang Lurong Jiangu Capsule	330:357	Fufang Lurong Jiangu Capsule (FLJC)	330:364	Fufang Lurong Jiangu Capsule (FLJC) has a known anti-osteoporotic effect, but its pharmacological effect on osteoblasts is not clearly understood.
31810119	8	41	dep	factor	1497:1502	arg1	Nrf2					1534:1537	Nrf2	1534:1537	Nrf2	1534:1537	The percentages of apoptosis were determined by flow cytometric analysis; apoptosis-related protein levels, including nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and heme oxygenase-1 (HO-1) with or without Nrf2 inhibitor were analyzed via western blot.
31810119	8	41	dep	factor	1497:1502	arg1	2					1531:1531	2	1531:1531	2	1531:1531	The percentages of apoptosis were determined by flow cytometric analysis; apoptosis-related protein levels, including nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and heme oxygenase-1 (HO-1) with or without Nrf2 inhibitor were analyzed via western blot.
31810119	15	42	from	loss	2508:2511	arg1	mice					2528:2531	LPS-induced mice	2516:2531	LPS-induced mice	2516:2531	Finally, we confirmed that FLJC administration could reverse the bone loss in LPS-induced mice.
31810119	5	43	theme	FLJC	783:786	arg1	preparations					788:799	FLJC preparations	783:799	FLJC preparations	783:799	METHODS Chemical compositions of FLJC preparations were analyzed using high-performance liquid chromatographic fingerprinting.
31810119	7	44	theme	enzymes	1254:1260	arg1	mineralization					1294:1307	mineralization	1294:1307	mineralization	1294:1307	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	7	44	theme	enzymes	1254:1260	arg1	levels					1145:1150	levels	1145:1150	levels of intracellular reactive oxygen species (ROS)	1145:1197	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	7	44	theme	enzymes	1254:1260	arg1	activity					1230:1237	activity	1230:1237	activity of antioxidant enzymes	1230:1260	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	7	44	theme	enzymes	1254:1260	arg1	expression					1337:1346	expression	1337:1346	expression of osteogenic markers	1337:1368	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	7	44	theme	enzymes	1254:1260	arg1	phosphatase					1272:1282	alkaline phosphatase	1263:1282	alkaline phosphatase (ALP)	1263:1288	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	7	44	theme	enzymes	1254:1260	arg1	viability					1111:1119	osteoblast cell viability	1095:1119	osteoblast cell viability	1095:1119	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	7	44	theme	enzymes	1254:1260	arg1	ALP					1285:1287	ALP	1285:1287	ALP	1285:1287	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	7	44	theme	enzymes	1254:1260	arg1	secretion					1314:1322	the secretion	1310:1322	the secretion of Col I	1310:1331	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	7	44	theme	enzymes	1254:1260	arg1	localization					1200:1211	localization	1200:1211	localization of mitochondria	1200:1227	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	16	45	theme	treatments	2738:2747	arg1	development					2723:2733	development	2723:2733	development of treatments for osteoporosis induced by oxidative injury	2723:2792	CONCLUSION These results indicate that FLJC may significantly attenuate oxidative damage of osteoblasts induced by H2O2 via the Nrf2/HO-1 signaling pathway, providing new insights to guide development of treatments for osteoporosis induced by oxidative injury.
31810119	8	46	theme	protein	1463:1469	arg1	factor					1497:1502	nuclear factor	1489:1502	nuclear factor (erythroid-derived 2)	1489:1524	The percentages of apoptosis were determined by flow cytometric analysis; apoptosis-related protein levels, including nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and heme oxygenase-1 (HO-1) with or without Nrf2 inhibitor were analyzed via western blot.
31810119	8	46	theme	protein	1463:1469	arg1	oxygenase-1					1549:1559	heme oxygenase-1	1544:1559	heme oxygenase-1 (HO-1)	1544:1566	The percentages of apoptosis were determined by flow cytometric analysis; apoptosis-related protein levels, including nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and heme oxygenase-1 (HO-1) with or without Nrf2 inhibitor were analyzed via western blot.
31810119	8	46	theme	protein	1463:1469	arg1	levels					1471:1476	apoptosis-related protein levels	1445:1476	apoptosis-related protein levels	1445:1476	The percentages of apoptosis were determined by flow cytometric analysis; apoptosis-related protein levels, including nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and heme oxygenase-1 (HO-1) with or without Nrf2 inhibitor were analyzed via western blot.
31810119	14	47	theme	protein	2422:2428	arg1	levels					2430:2435	Nrf2 and HO-1 protein levels	2408:2435	Nrf2 and HO-1 protein levels	2408:2435	Furthermore, FLJC protected cells from H2O2-induced oxidative damage by up-regulating Nrf2 and HO-1 protein levels.
31810119	0	48	theme	marrow	113:118	arg1	osteoblasts					141:151	bone marrow stromal cell-derived osteoblasts	108:151	bone marrow stromal cell-derived osteoblasts	108:151	Cytoprotective effect of Fufang Lurong Jiangu capsule against hydrogen peroxide-induced oxidative stress in bone marrow stromal cell-derived osteoblasts through the Nrf2/HO-1 signaling pathway.
31810119	0	49	theme	Jiangu	39:44	arg1	capsule					46:52	Fufang Lurong Jiangu capsule	25:52	Fufang Lurong Jiangu capsule	25:52	Cytoprotective effect of Fufang Lurong Jiangu capsule against hydrogen peroxide-induced oxidative stress in bone marrow stromal cell-derived osteoblasts through the Nrf2/HO-1 signaling pathway.
31810119	0	50	theme	Cytoprotective	0:13	arg1	effect					15:20	Cytoprotective effect	0:20	Cytoprotective effect of Fufang Lurong Jiangu capsule against hydrogen peroxide-induced oxidative stress in bone marrow stromal cell-derived osteoblasts through the Nrf2/HO-1 signaling pathway.	0:192	Cytoprotective effect of Fufang Lurong Jiangu capsule against hydrogen peroxide-induced oxidative stress in bone marrow stromal cell-derived osteoblasts through the Nrf2/HO-1 signaling pathway.
31810119	8	51	theme	western	1617:1623	arg1	blot					1625:1628	western blot	1617:1628	western blot	1617:1628	The percentages of apoptosis were determined by flow cytometric analysis; apoptosis-related protein levels, including nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and heme oxygenase-1 (HO-1) with or without Nrf2 inhibitor were analyzed via western blot.
31810119	8	52	theme	nuclear	1489:1495	arg1	erythroid-derived					1505:1521	erythroid-derived 2	1505:1523	erythroid-derived 2	1505:1523	The percentages of apoptosis were determined by flow cytometric analysis; apoptosis-related protein levels, including nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and heme oxygenase-1 (HO-1) with or without Nrf2 inhibitor were analyzed via western blot.
31810119	8	52	theme	nuclear	1489:1495	arg1	factor					1497:1502	nuclear factor	1489:1502	nuclear factor (erythroid-derived 2)	1489:1524	The percentages of apoptosis were determined by flow cytometric analysis; apoptosis-related protein levels, including nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and heme oxygenase-1 (HO-1) with or without Nrf2 inhibitor were analyzed via western blot.
31810119	0	53	theme	Lurong	32:37	arg1	capsule					46:52	Fufang Lurong Jiangu capsule	25:52	Fufang Lurong Jiangu capsule	25:52	Cytoprotective effect of Fufang Lurong Jiangu capsule against hydrogen peroxide-induced oxidative stress in bone marrow stromal cell-derived osteoblasts through the Nrf2/HO-1 signaling pathway.
31810119	6	54	theme	resulting	948:956	arg1	osteoblasts					958:968	resulting osteoblasts	948:968	resulting osteoblasts	948:968	After rat bone marrow mesenchymal stem cell differentiation induction, resulting osteoblasts received various 48 h FLJC pretreatments before H2O2-based (200 μM) oxidative stress exposure.
31810119	0	55	theme	hydrogen	62:69	arg1	stress					98:103	hydrogen peroxide-induced oxidative stress	62:103	hydrogen peroxide-induced oxidative stress in bone marrow stromal cell-derived osteoblasts through the Nrf2/HO-1 signaling pathway	62:191	Cytoprotective effect of Fufang Lurong Jiangu capsule against hydrogen peroxide-induced oxidative stress in bone marrow stromal cell-derived osteoblasts through the Nrf2/HO-1 signaling pathway.
31810119	10	56	dep	RESULTS	1736:1742	arg1	components					1758:1767	Five chemical components	1744:1767	RESULTS Five chemical components in FLJC	1736:1775	RESULTS Five chemical components in FLJC were identified, and fingerprint analysis showed good reproducibility.
31810119	11	57	theme	antioxidant	1941:1951	arg1	activities					1960:1969	increased antioxidant enzyme activities	1931:1969	increased antioxidant enzyme activities	1931:1969	FLJC pretreatment significantly reduced H2O2-induced ROS levels in osteoblasts and increased antioxidant enzyme activities to reduce oxidative damage.
31810119	0	58	theme	capsule	46:52	arg1	effect					15:20	Cytoprotective effect	0:20	Cytoprotective effect of Fufang Lurong Jiangu capsule against hydrogen peroxide-induced oxidative stress in bone marrow stromal cell-derived osteoblasts through the Nrf2/HO-1 signaling pathway.	0:192	Cytoprotective effect of Fufang Lurong Jiangu capsule against hydrogen peroxide-induced oxidative stress in bone marrow stromal cell-derived osteoblasts through the Nrf2/HO-1 signaling pathway.
31810119	2	59	from	effect	428:433	arg1	osteoblasts					438:448	osteoblasts	438:448	osteoblasts	438:448	Fufang Lurong Jiangu Capsule (FLJC) has a known anti-osteoporotic effect, but its pharmacological effect on osteoblasts is not clearly understood.
31810119	16	60	theme	Nrf2/HO-1	2662:2670	arg1	pathway					2682:2688	the Nrf2/HO-1 signaling pathway	2658:2688	the Nrf2/HO-1 signaling pathway	2658:2688	CONCLUSION These results indicate that FLJC may significantly attenuate oxidative damage of osteoblasts induced by H2O2 via the Nrf2/HO-1 signaling pathway, providing new insights to guide development of treatments for osteoporosis induced by oxidative injury.
31810119	10	61	theme	chemical	1749:1756	arg1	components					1758:1767	Five chemical components	1744:1767	RESULTS Five chemical components in FLJC	1736:1775	RESULTS Five chemical components in FLJC were identified, and fingerprint analysis showed good reproducibility.
31810119	1	62	theme	risk	253:256	arg1	stress					214:219	OBJECTIVE Oxidative stress	194:219	OBJECTIVE Oxidative stress	194:219	OBJECTIVE Oxidative stress is increasingly recognized as a risk factor associated with the development and progression of osteoporosis.
31810119	1	62	theme	risk	253:256	arg1	factor					258:263	a risk factor	251:263	a risk factor associated with the development and progression of osteoporosis	251:327	OBJECTIVE Oxidative stress is increasingly recognized as a risk factor associated with the development and progression of osteoporosis.
31810119	6	63	theme	cell	916:919	arg1	differentiation					921:935	rat bone marrow mesenchymal stem cell differentiation	883:935	rat bone marrow mesenchymal stem cell differentiation induction	883:945	After rat bone marrow mesenchymal stem cell differentiation induction, resulting osteoblasts received various 48 h FLJC pretreatments before H2O2-based (200 μM) oxidative stress exposure.
31810119	9	64	theme	in	1685:1686	arg1	effect					1698:1703	in vivo FLJC effect	1685:1703	in vivo FLJC effect on mice	1685:1711	Hematoxylin and eosin (H&E) and ALP staining revealed in vivo FLJC effect on mice LPS-induced bone loss.
31810119	7	65	theme	markers	1362:1368	arg1	mineralization					1294:1307	mineralization	1294:1307	mineralization	1294:1307	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	7	65	theme	markers	1362:1368	arg1	levels					1145:1150	levels	1145:1150	levels of intracellular reactive oxygen species (ROS)	1145:1197	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	7	65	theme	markers	1362:1368	arg1	activity					1230:1237	activity	1230:1237	activity of antioxidant enzymes	1230:1260	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	7	65	theme	markers	1362:1368	arg1	expression					1337:1346	expression	1337:1346	expression of osteogenic markers	1337:1368	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	7	65	theme	markers	1362:1368	arg1	phosphatase					1272:1282	alkaline phosphatase	1263:1282	alkaline phosphatase (ALP)	1263:1288	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	7	65	theme	markers	1362:1368	arg1	viability					1111:1119	osteoblast cell viability	1095:1119	osteoblast cell viability	1095:1119	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	7	65	theme	markers	1362:1368	arg1	ALP					1285:1287	ALP	1285:1287	ALP	1285:1287	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	7	65	theme	markers	1362:1368	arg1	secretion					1314:1322	the secretion	1310:1322	the secretion of Col I	1310:1331	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	7	65	theme	markers	1362:1368	arg1	localization					1200:1211	localization	1200:1211	localization of mitochondria	1200:1227	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	14	66	theme	oxidative	2374:2382	arg1	damage					2384:2389	H2O2-induced oxidative damage	2361:2389	H2O2-induced oxidative damage	2361:2389	Furthermore, FLJC protected cells from H2O2-induced oxidative damage by up-regulating Nrf2 and HO-1 protein levels.
31810119	3	67	theme	hydrogen	552:559	arg1	H2O2					571:574	H2O2	571:574	H2O2	571:574	This study was designed to investigate FLJC effects/mechanisms on in vitro hydrogen peroxide (H2O2)-induced oxidative damage of osteoblasts and on in vivo lipopolysaccharide (LPS)-induced mice bone loss.
31810119	3	67	theme	hydrogen	552:559	arg1	peroxide					561:568	in vitro hydrogen peroxide	543:568	in vitro hydrogen peroxide (H2O2)-induced oxidative damage of osteoblasts	543:615	This study was designed to investigate FLJC effects/mechanisms on in vitro hydrogen peroxide (H2O2)-induced oxidative damage of osteoblasts and on in vivo lipopolysaccharide (LPS)-induced mice bone loss.
31810119	13	68	theme	pathway	2313:2319	arg1	proteins					2273:2280	major Bcl-2 family effector proteins	2245:2280	major Bcl-2 family effector proteins of the mitochondrial apoptosis pathway	2245:2319	Additionally, FLJC protected against H2O2-induced apoptosis by inhibiting changes in expression of major Bcl-2 family effector proteins of the mitochondrial apoptosis pathway.
31810119	9	69	theme	FLJC	1693:1696	arg1	effect					1698:1703	in vivo FLJC effect	1685:1703	in vivo FLJC effect on mice	1685:1711	Hematoxylin and eosin (H&E) and ALP staining revealed in vivo FLJC effect on mice LPS-induced bone loss.
31810119	2	70	theme	anti-osteoporotic	378:394	arg1	effect					396:401	a known anti-osteoporotic effect	370:401	a known anti-osteoporotic effect	370:401	Fufang Lurong Jiangu Capsule (FLJC) has a known anti-osteoporotic effect, but its pharmacological effect on osteoblasts is not clearly understood.
31810119	13	71	theme	effector	2264:2271	arg1	proteins					2273:2280	major Bcl-2 family effector proteins	2245:2280	major Bcl-2 family effector proteins of the mitochondrial apoptosis pathway	2245:2319	Additionally, FLJC protected against H2O2-induced apoptosis by inhibiting changes in expression of major Bcl-2 family effector proteins of the mitochondrial apoptosis pathway.
31810119	12	72	theme	markers	2137:2143	arg1	levels					2097:2102	levels	2097:2102	levels of mineralization and osteoblast markers	2097:2143	With regard to osteoblast differentiation, FLJC pretreatment increased ALP expression, as well as levels of mineralization and osteoblast markers.
31810119	12	72	theme	markers	2137:2143	arg1	expression					2074:2083	ALP expression	2070:2083	ALP expression	2070:2083	With regard to osteoblast differentiation, FLJC pretreatment increased ALP expression, as well as levels of mineralization and osteoblast markers.
31810119	6	73	theme	H2O2-based	1018:1027	arg1	exposure					1055:1062	H2O2-based (200 μM) oxidative stress exposure	1018:1062	H2O2-based (200 μM) oxidative stress exposure	1018:1062	After rat bone marrow mesenchymal stem cell differentiation induction, resulting osteoblasts received various 48 h FLJC pretreatments before H2O2-based (200 μM) oxidative stress exposure.
31810119	11	74	from	levels	1905:1910	arg1	activities					1960:1969	increased antioxidant enzyme activities	1931:1969	increased antioxidant enzyme activities	1931:1969	FLJC pretreatment significantly reduced H2O2-induced ROS levels in osteoblasts and increased antioxidant enzyme activities to reduce oxidative damage.
31810119	11	74	from	levels	1905:1910	arg1	osteoblasts					1915:1925	osteoblasts	1915:1925	osteoblasts	1915:1925	FLJC pretreatment significantly reduced H2O2-induced ROS levels in osteoblasts and increased antioxidant enzyme activities to reduce oxidative damage.
31810119	6	75	theme	marrow	892:897	arg1	differentiation					921:935	rat bone marrow mesenchymal stem cell differentiation	883:935	rat bone marrow mesenchymal stem cell differentiation induction	883:945	After rat bone marrow mesenchymal stem cell differentiation induction, resulting osteoblasts received various 48 h FLJC pretreatments before H2O2-based (200 μM) oxidative stress exposure.
31810119	2	76	theme	Fufang	330:335	arg1	FLJC					360:363	FLJC	360:363	FLJC	360:363	Fufang Lurong Jiangu Capsule (FLJC) has a known anti-osteoporotic effect, but its pharmacological effect on osteoblasts is not clearly understood.
31810119	2	76	theme	Fufang	330:335	arg1	Capsule					351:357	Fufang Lurong Jiangu Capsule	330:357	Fufang Lurong Jiangu Capsule (FLJC)	330:364	Fufang Lurong Jiangu Capsule (FLJC) has a known anti-osteoporotic effect, but its pharmacological effect on osteoblasts is not clearly understood.
31810119	16	77	theme	osteoblasts	2626:2636	arg1	damage					2616:2621	oxidative damage	2606:2621	oxidative damage of osteoblasts induced by H2O2 via the Nrf2/HO-1 signaling pathway	2606:2688	CONCLUSION These results indicate that FLJC may significantly attenuate oxidative damage of osteoblasts induced by H2O2 via the Nrf2/HO-1 signaling pathway, providing new insights to guide development of treatments for osteoporosis induced by oxidative injury.
31810119	9	78	theme	LPS-induced	1713:1723	arg1	loss					1730:1733	LPS-induced bone loss	1713:1733	LPS-induced bone loss	1713:1733	Hematoxylin and eosin (H&E) and ALP staining revealed in vivo FLJC effect on mice LPS-induced bone loss.
31810119	6	79	theme	rat	883:885	arg1	marrow					892:897	rat bone marrow	883:897	rat bone marrow mesenchymal stem cell differentiation induction	883:945	After rat bone marrow mesenchymal stem cell differentiation induction, resulting osteoblasts received various 48 h FLJC pretreatments before H2O2-based (200 μM) oxidative stress exposure.
31810119	10	80	theme	fingerprint	1798:1808	arg1	analysis					1810:1817	fingerprint analysis	1798:1817	fingerprint analysis	1798:1817	RESULTS Five chemical components in FLJC were identified, and fingerprint analysis showed good reproducibility.
31810119	1	81	theme	OBJECTIVE	194:202	arg1	stress					214:219	OBJECTIVE Oxidative stress	194:219	OBJECTIVE Oxidative stress	194:219	OBJECTIVE Oxidative stress is increasingly recognized as a risk factor associated with the development and progression of osteoporosis.
31810119	1	81	theme	OBJECTIVE	194:202	arg1	factor					258:263	a risk factor	251:263	a risk factor associated with the development and progression of osteoporosis	251:327	OBJECTIVE Oxidative stress is increasingly recognized as a risk factor associated with the development and progression of osteoporosis.
31810119	7	82	theme	antioxidant	1242:1252	arg1	enzymes					1254:1260	antioxidant enzymes	1242:1260	antioxidant enzymes	1242:1260	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	6	83	theme	48 h	987:990	arg1	pretreatments					997:1009	various 48 h FLJC pretreatments	979:1009	various 48 h FLJC pretreatments	979:1009	After rat bone marrow mesenchymal stem cell differentiation induction, resulting osteoblasts received various 48 h FLJC pretreatments before H2O2-based (200 μM) oxidative stress exposure.
31810119	9	84	from	effect	1698:1703	arg1	mice					1708:1711	mice	1708:1711	mice	1708:1711	Hematoxylin and eosin (H&E) and ALP staining revealed in vivo FLJC effect on mice LPS-induced bone loss.
31810119	5	85	theme	liquid	838:843	arg1	fingerprinting					861:874	high-performance liquid chromatographic fingerprinting	821:874	high-performance liquid chromatographic fingerprinting	821:874	METHODS Chemical compositions of FLJC preparations were analyzed using high-performance liquid chromatographic fingerprinting.
31810119	3	86	dep	in	624:625	arg1	vivo					627:630	vivo	627:630	vivo	627:630	This study was designed to investigate FLJC effects/mechanisms on in vitro hydrogen peroxide (H2O2)-induced oxidative damage of osteoblasts and on in vivo lipopolysaccharide (LPS)-induced mice bone loss.
31810119	11	87	theme	H2O2-induced	1888:1899	arg1	levels					1905:1910	H2O2-induced ROS levels	1888:1910	H2O2-induced ROS levels in osteoblasts and increased antioxidant enzyme activities	1888:1969	FLJC pretreatment significantly reduced H2O2-induced ROS levels in osteoblasts and increased antioxidant enzyme activities to reduce oxidative damage.
31810119	7	88	theme	species	1185:1191	arg1	mineralization					1294:1307	mineralization	1294:1307	mineralization	1294:1307	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	7	88	theme	species	1185:1191	arg1	levels					1145:1150	levels	1145:1150	levels of intracellular reactive oxygen species (ROS)	1145:1197	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	7	88	theme	species	1185:1191	arg1	activity					1230:1237	activity	1230:1237	activity of antioxidant enzymes	1230:1260	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	7	88	theme	species	1185:1191	arg1	expression					1337:1346	expression	1337:1346	expression of osteogenic markers	1337:1368	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	7	88	theme	species	1185:1191	arg1	phosphatase					1272:1282	alkaline phosphatase	1263:1282	alkaline phosphatase (ALP)	1263:1288	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	7	88	theme	species	1185:1191	arg1	viability					1111:1119	osteoblast cell viability	1095:1119	osteoblast cell viability	1095:1119	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	7	88	theme	species	1185:1191	arg1	ALP					1285:1287	ALP	1285:1287	ALP	1285:1287	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	7	88	theme	species	1185:1191	arg1	secretion					1314:1322	the secretion	1310:1322	the secretion of Col I	1310:1331	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	7	88	theme	species	1185:1191	arg1	localization					1200:1211	localization	1200:1211	localization of mitochondria	1200:1227	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	2	89	theme	Jiangu	344:349	arg1	FLJC					360:363	FLJC	360:363	FLJC	360:363	Fufang Lurong Jiangu Capsule (FLJC) has a known anti-osteoporotic effect, but its pharmacological effect on osteoblasts is not clearly understood.
31810119	2	89	theme	Jiangu	344:349	arg1	Capsule					351:357	Fufang Lurong Jiangu Capsule	330:357	Fufang Lurong Jiangu Capsule (FLJC)	330:364	Fufang Lurong Jiangu Capsule (FLJC) has a known anti-osteoporotic effect, but its pharmacological effect on osteoblasts is not clearly understood.
31810119	16	90	theme	oxidative	2777:2785	arg1	injury					2787:2792	oxidative injury	2777:2792	oxidative injury	2777:2792	CONCLUSION These results indicate that FLJC may significantly attenuate oxidative damage of osteoblasts induced by H2O2 via the Nrf2/HO-1 signaling pathway, providing new insights to guide development of treatments for osteoporosis induced by oxidative injury.
31810119	0	91	from	stress	98:103	arg1	osteoblasts					141:151	bone marrow stromal cell-derived osteoblasts	108:151	bone marrow stromal cell-derived osteoblasts	108:151	Cytoprotective effect of Fufang Lurong Jiangu capsule against hydrogen peroxide-induced oxidative stress in bone marrow stromal cell-derived osteoblasts through the Nrf2/HO-1 signaling pathway.
31810119	8	92	theme	flow	1419:1422	arg1	analysis					1435:1442	flow cytometric analysis	1419:1442	flow cytometric analysis	1419:1442	The percentages of apoptosis were determined by flow cytometric analysis; apoptosis-related protein levels, including nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and heme oxygenase-1 (HO-1) with or without Nrf2 inhibitor were analyzed via western blot.
31810119	3	93	theme	in	543:544	arg1	H2O2					571:574	H2O2	571:574	H2O2	571:574	This study was designed to investigate FLJC effects/mechanisms on in vitro hydrogen peroxide (H2O2)-induced oxidative damage of osteoblasts and on in vivo lipopolysaccharide (LPS)-induced mice bone loss.
31810119	3	93	theme	in	543:544	arg1	peroxide					561:568	in vitro hydrogen peroxide	543:568	in vitro hydrogen peroxide (H2O2)-induced oxidative damage of osteoblasts	543:615	This study was designed to investigate FLJC effects/mechanisms on in vitro hydrogen peroxide (H2O2)-induced oxidative damage of osteoblasts and on in vivo lipopolysaccharide (LPS)-induced mice bone loss.
31810119	7	94	theme	reactive	1169:1176	arg1	ROS					1194:1196	ROS	1194:1196	ROS	1194:1196	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	7	94	theme	reactive	1169:1176	arg1	species					1185:1191	intracellular reactive oxygen species	1155:1191	intracellular reactive oxygen species (ROS)	1155:1197	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	13	95	theme	major	2245:2249	arg1	proteins					2273:2280	major Bcl-2 family effector proteins	2245:2280	major Bcl-2 family effector proteins of the mitochondrial apoptosis pathway	2245:2319	Additionally, FLJC protected against H2O2-induced apoptosis by inhibiting changes in expression of major Bcl-2 family effector proteins of the mitochondrial apoptosis pathway.
31810119	3	96	theme	in	624:625	arg1	LPS					652:654	LPS	652:654	LPS	652:654	This study was designed to investigate FLJC effects/mechanisms on in vitro hydrogen peroxide (H2O2)-induced oxidative damage of osteoblasts and on in vivo lipopolysaccharide (LPS)-induced mice bone loss.
31810119	3	96	theme	in	624:625	arg1	lipopolysaccharide					632:649	in vivo lipopolysaccharide	624:649	in vivo lipopolysaccharide (LPS)	624:655	This study was designed to investigate FLJC effects/mechanisms on in vitro hydrogen peroxide (H2O2)-induced oxidative damage of osteoblasts and on in vivo lipopolysaccharide (LPS)-induced mice bone loss.
31810119	12	97	theme	FLJC	2042:2045	arg1	pretreatment					2047:2058	FLJC pretreatment	2042:2058	FLJC pretreatment	2042:2058	With regard to osteoblast differentiation, FLJC pretreatment increased ALP expression, as well as levels of mineralization and osteoblast markers.
31810119	8	98	theme	heme	1544:1547	arg1	HO-1					1562:1565	HO-1	1562:1565	HO-1	1562:1565	The percentages of apoptosis were determined by flow cytometric analysis; apoptosis-related protein levels, including nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and heme oxygenase-1 (HO-1) with or without Nrf2 inhibitor were analyzed via western blot.
31810119	8	98	theme	heme	1544:1547	arg1	oxygenase-1					1549:1559	heme oxygenase-1	1544:1559	heme oxygenase-1 (HO-1)	1544:1566	The percentages of apoptosis were determined by flow cytometric analysis; apoptosis-related protein levels, including nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and heme oxygenase-1 (HO-1) with or without Nrf2 inhibitor were analyzed via western blot.
31810119	9	99	dep	in	1685:1686	arg1	vivo					1688:1691	vivo	1688:1691	vivo	1688:1691	Hematoxylin and eosin (H&E) and ALP staining revealed in vivo FLJC effect on mice LPS-induced bone loss.
31810119	0	100	theme	signaling	175:183	arg1	pathway					185:191	the Nrf2/HO-1 signaling pathway	161:191	the Nrf2/HO-1 signaling pathway	161:191	Cytoprotective effect of Fufang Lurong Jiangu capsule against hydrogen peroxide-induced oxidative stress in bone marrow stromal cell-derived osteoblasts through the Nrf2/HO-1 signaling pathway.
31810119	13	101	theme	family	2257:2262	arg1	proteins					2273:2280	major Bcl-2 family effector proteins	2245:2280	major Bcl-2 family effector proteins of the mitochondrial apoptosis pathway	2245:2319	Additionally, FLJC protected against H2O2-induced apoptosis by inhibiting changes in expression of major Bcl-2 family effector proteins of the mitochondrial apoptosis pathway.
31810119	6	102	theme	oxidative	1038:1046	arg1	exposure					1055:1062	H2O2-based (200 μM) oxidative stress exposure	1018:1062	H2O2-based (200 μM) oxidative stress exposure	1018:1062	After rat bone marrow mesenchymal stem cell differentiation induction, resulting osteoblasts received various 48 h FLJC pretreatments before H2O2-based (200 μM) oxidative stress exposure.
31810119	16	103	theme	signaling	2672:2680	arg1	pathway					2682:2688	the Nrf2/HO-1 signaling pathway	2658:2688	the Nrf2/HO-1 signaling pathway	2658:2688	CONCLUSION These results indicate that FLJC may significantly attenuate oxidative damage of osteoblasts induced by H2O2 via the Nrf2/HO-1 signaling pathway, providing new insights to guide development of treatments for osteoporosis induced by oxidative injury.
31810119	15	104	theme	bone	2503:2506	arg1	loss					2508:2511	the bone loss	2499:2511	the bone loss in LPS-induced mice	2499:2531	Finally, we confirmed that FLJC administration could reverse the bone loss in LPS-induced mice.
31810119	3	105	theme	oxidative	585:593	arg1	damage					595:600	in vitro hydrogen peroxide (H2O2)-induced oxidative damage	543:600	in vitro hydrogen peroxide (H2O2)-induced oxidative damage of osteoblasts	543:615	This study was designed to investigate FLJC effects/mechanisms on in vitro hydrogen peroxide (H2O2)-induced oxidative damage of osteoblasts and on in vivo lipopolysaccharide (LPS)-induced mice bone loss.
31810119	8	106	theme	apoptosis	1390:1398	arg1	percentages					1375:1385	The percentages	1371:1385	The percentages of apoptosis	1371:1398	The percentages of apoptosis were determined by flow cytometric analysis; apoptosis-related protein levels, including nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and heme oxygenase-1 (HO-1) with or without Nrf2 inhibitor were analyzed via western blot.
31810119	0	107	theme	peroxide-induced	71:86	arg1	stress					98:103	hydrogen peroxide-induced oxidative stress	62:103	hydrogen peroxide-induced oxidative stress in bone marrow stromal cell-derived osteoblasts through the Nrf2/HO-1 signaling pathway	62:191	Cytoprotective effect of Fufang Lurong Jiangu capsule against hydrogen peroxide-induced oxidative stress in bone marrow stromal cell-derived osteoblasts through the Nrf2/HO-1 signaling pathway.
31810119	7	108	theme	cell	1106:1109	arg1	levels					1145:1150	levels	1145:1150	levels of intracellular reactive oxygen species (ROS)	1145:1197	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	7	108	theme	cell	1106:1109	arg1	activity					1230:1237	activity	1230:1237	activity of antioxidant enzymes	1230:1260	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	7	108	theme	cell	1106:1109	arg1	expression					1337:1346	expression	1337:1346	expression of osteogenic markers	1337:1368	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	7	108	theme	cell	1106:1109	arg1	viability					1111:1119	osteoblast cell viability	1095:1119	osteoblast cell viability	1095:1119	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	7	108	theme	cell	1106:1109	arg1	secretion					1314:1322	the secretion	1310:1322	the secretion of Col I	1310:1331	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	7	108	theme	cell	1106:1109	arg1	localization					1200:1211	localization	1200:1211	localization of mitochondria	1200:1227	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	7	108	theme	cell	1106:1109	arg1	changes					1136:1142	morphological changes	1122:1142	morphological changes	1122:1142	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	13	109	theme	mitochondrial	2289:2301	arg1	pathway					2313:2319	the mitochondrial apoptosis pathway	2285:2319	the mitochondrial apoptosis pathway	2285:2319	Additionally, FLJC protected against H2O2-induced apoptosis by inhibiting changes in expression of major Bcl-2 family effector proteins of the mitochondrial apoptosis pathway.
31810119	5	110	theme	high-performance	821:836	arg1	fingerprinting					861:874	high-performance liquid chromatographic fingerprinting	821:874	high-performance liquid chromatographic fingerprinting	821:874	METHODS Chemical compositions of FLJC preparations were analyzed using high-performance liquid chromatographic fingerprinting.
31810119	13	111	theme	H2O2-induced	2183:2194	arg1	apoptosis					2196:2204	H2O2-induced apoptosis	2183:2204	H2O2-induced apoptosis	2183:2204	Additionally, FLJC protected against H2O2-induced apoptosis by inhibiting changes in expression of major Bcl-2 family effector proteins of the mitochondrial apoptosis pathway.
31810119	12	112	theme	osteoblast	2014:2023	arg1	differentiation					2025:2039	osteoblast differentiation	2014:2039	osteoblast differentiation	2014:2039	With regard to osteoblast differentiation, FLJC pretreatment increased ALP expression, as well as levels of mineralization and osteoblast markers.
31810119	0	113	theme	cell-derived	128:139	arg1	osteoblasts					141:151	bone marrow stromal cell-derived osteoblasts	108:151	bone marrow stromal cell-derived osteoblasts	108:151	Cytoprotective effect of Fufang Lurong Jiangu capsule against hydrogen peroxide-induced oxidative stress in bone marrow stromal cell-derived osteoblasts through the Nrf2/HO-1 signaling pathway.
31810119	8	114	theme	Nrf2	1584:1587	arg1	inhibitor					1589:1597	Nrf2 inhibitor	1584:1597	Nrf2 inhibitor	1584:1597	The percentages of apoptosis were determined by flow cytometric analysis; apoptosis-related protein levels, including nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and heme oxygenase-1 (HO-1) with or without Nrf2 inhibitor were analyzed via western blot.
31810119	4	115	theme	pharmacological	722:736	arg1	mechanisms					738:747	unknown pharmacological mechanisms	714:747	unknown pharmacological mechanisms	714:747	FLJC alleviates osteoporosis via unknown pharmacological mechanisms.
31810119	11	116	theme	FLJC	1848:1851	arg1	pretreatment					1853:1864	FLJC pretreatment	1848:1864	FLJC pretreatment	1848:1864	FLJC pretreatment significantly reduced H2O2-induced ROS levels in osteoblasts and increased antioxidant enzyme activities to reduce oxidative damage.
31810119	0	117	theme	bone	108:111	arg1	marrow					113:118	bone marrow	108:118	bone marrow stromal cell-derived osteoblasts	108:151	Cytoprotective effect of Fufang Lurong Jiangu capsule against hydrogen peroxide-induced oxidative stress in bone marrow stromal cell-derived osteoblasts through the Nrf2/HO-1 signaling pathway.
31810119	10	118	theme	good	1826:1829	arg1	reproducibility					1831:1845	good reproducibility	1826:1845	good reproducibility	1826:1845	RESULTS Five chemical components in FLJC were identified, and fingerprint analysis showed good reproducibility.
31810119	11	119	theme	increased	1931:1939	arg1	activities					1960:1969	increased antioxidant enzyme activities	1931:1969	increased antioxidant enzyme activities	1931:1969	FLJC pretreatment significantly reduced H2O2-induced ROS levels in osteoblasts and increased antioxidant enzyme activities to reduce oxidative damage.
31810119	5	120	theme	preparations	788:799	arg1	compositions					767:778	METHODS Chemical compositions	750:778	METHODS Chemical compositions of FLJC preparations	750:799	METHODS Chemical compositions of FLJC preparations were analyzed using high-performance liquid chromatographic fingerprinting.
31810119	7	121	theme	FLJC	1065:1068	arg1	effects					1070:1076	FLJC effects	1065:1076	FLJC effects	1065:1076	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	8	122	theme	apoptosis-related	1445:1461	arg1	factor					1497:1502	nuclear factor	1489:1502	nuclear factor (erythroid-derived 2)	1489:1524	The percentages of apoptosis were determined by flow cytometric analysis; apoptosis-related protein levels, including nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and heme oxygenase-1 (HO-1) with or without Nrf2 inhibitor were analyzed via western blot.
31810119	8	122	theme	apoptosis-related	1445:1461	arg1	oxygenase-1					1549:1559	heme oxygenase-1	1544:1559	heme oxygenase-1 (HO-1)	1544:1566	The percentages of apoptosis were determined by flow cytometric analysis; apoptosis-related protein levels, including nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and heme oxygenase-1 (HO-1) with or without Nrf2 inhibitor were analyzed via western blot.
31810119	8	122	theme	apoptosis-related	1445:1461	arg1	levels					1471:1476	apoptosis-related protein levels	1445:1476	apoptosis-related protein levels	1445:1476	The percentages of apoptosis were determined by flow cytometric analysis; apoptosis-related protein levels, including nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and heme oxygenase-1 (HO-1) with or without Nrf2 inhibitor were analyzed via western blot.
31810119	14	123	theme	HO-1	2417:2420	arg1	levels					2430:2435	Nrf2 and HO-1 protein levels	2408:2435	Nrf2 and HO-1 protein levels	2408:2435	Furthermore, FLJC protected cells from H2O2-induced oxidative damage by up-regulating Nrf2 and HO-1 protein levels.
31810119	0	124	theme	stromal	120:126	arg1	osteoblasts					141:151	bone marrow stromal cell-derived osteoblasts	108:151	bone marrow stromal cell-derived osteoblasts	108:151	Cytoprotective effect of Fufang Lurong Jiangu capsule against hydrogen peroxide-induced oxidative stress in bone marrow stromal cell-derived osteoblasts through the Nrf2/HO-1 signaling pathway.
31810119	3	125	dep	in	543:544	arg1	vitro					546:550	vitro	546:550	vitro	546:550	This study was designed to investigate FLJC effects/mechanisms on in vitro hydrogen peroxide (H2O2)-induced oxidative damage of osteoblasts and on in vivo lipopolysaccharide (LPS)-induced mice bone loss.
31810119	0	126	link	cell-derived	128:139	arg1	osteoblasts					141:151	bone marrow stromal cell-derived osteoblasts	108:151	bone marrow stromal cell-derived osteoblasts	108:151	Cytoprotective effect of Fufang Lurong Jiangu capsule against hydrogen peroxide-induced oxidative stress in bone marrow stromal cell-derived osteoblasts through the Nrf2/HO-1 signaling pathway.
31810119	5	127	theme	Chemical	758:765	arg1	compositions					767:778	METHODS Chemical compositions	750:778	METHODS Chemical compositions of FLJC preparations	750:799	METHODS Chemical compositions of FLJC preparations were analyzed using high-performance liquid chromatographic fingerprinting.
31810119	14	128	theme	Nrf2	2408:2411	arg1	levels					2430:2435	Nrf2 and HO-1 protein levels	2408:2435	Nrf2 and HO-1 protein levels	2408:2435	Furthermore, FLJC protected cells from H2O2-induced oxidative damage by up-regulating Nrf2 and HO-1 protein levels.
31810119	0	129	theme	Fufang	25:30	arg1	capsule					46:52	Fufang Lurong Jiangu capsule	25:52	Fufang Lurong Jiangu capsule	25:52	Cytoprotective effect of Fufang Lurong Jiangu capsule against hydrogen peroxide-induced oxidative stress in bone marrow stromal cell-derived osteoblasts through the Nrf2/HO-1 signaling pathway.
31810119	1	130	theme	osteoporosis	316:327	arg1	development					285:295	development	285:295	development	285:295	OBJECTIVE Oxidative stress is increasingly recognized as a risk factor associated with the development and progression of osteoporosis.
31810119	1	130	theme	osteoporosis	316:327	arg1	progression					301:311	progression	301:311	progression	301:311	OBJECTIVE Oxidative stress is increasingly recognized as a risk factor associated with the development and progression of osteoporosis.
31810119	3	131	theme	bone	670:673	arg1	loss					675:678	bone loss	670:678	bone loss	670:678	This study was designed to investigate FLJC effects/mechanisms on in vitro hydrogen peroxide (H2O2)-induced oxidative damage of osteoblasts and on in vivo lipopolysaccharide (LPS)-induced mice bone loss.
31810119	5	132	theme	chromatographic	845:859	arg1	fingerprinting					861:874	high-performance liquid chromatographic fingerprinting	821:874	high-performance liquid chromatographic fingerprinting	821:874	METHODS Chemical compositions of FLJC preparations were analyzed using high-performance liquid chromatographic fingerprinting.
31810119	3	133	theme	-induced	656:663	arg1	mice					665:668	in vivo lipopolysaccharide (LPS)-induced mice	624:668	in vivo lipopolysaccharide (LPS)-induced mice	624:668	This study was designed to investigate FLJC effects/mechanisms on in vitro hydrogen peroxide (H2O2)-induced oxidative damage of osteoblasts and on in vivo lipopolysaccharide (LPS)-induced mice bone loss.
31810119	9	134	theme	ALP	1663:1665	arg1	staining					1667:1674	ALP staining	1663:1674	ALP staining	1663:1674	Hematoxylin and eosin (H&E) and ALP staining revealed in vivo FLJC effect on mice LPS-induced bone loss.
31810119	11	135	theme	enzyme	1953:1958	arg1	activities					1960:1969	increased antioxidant enzyme activities	1931:1969	increased antioxidant enzyme activities	1931:1969	FLJC pretreatment significantly reduced H2O2-induced ROS levels in osteoblasts and increased antioxidant enzyme activities to reduce oxidative damage.
31810119	6	136	theme	differentiation	921:935	arg1	induction					937:945	rat bone marrow mesenchymal stem cell differentiation induction	883:945	rat bone marrow mesenchymal stem cell differentiation induction	883:945	After rat bone marrow mesenchymal stem cell differentiation induction, resulting osteoblasts received various 48 h FLJC pretreatments before H2O2-based (200 μM) oxidative stress exposure.
31810119	7	137	theme	osteogenic	1351:1360	arg1	markers					1362:1368	osteogenic markers	1351:1368	osteogenic markers	1351:1368	FLJC effects were measured on osteoblast cell viability, morphological changes, levels of intracellular reactive oxygen species (ROS), localization of mitochondria, activity of antioxidant enzymes, alkaline phosphatase (ALP) and mineralization, the secretion of Col I and expression of osteogenic markers.
31810119	3	138	theme	FLJC	516:519	arg1	effects/mechanisms					521:538	FLJC effects/mechanisms	516:538	FLJC effects/mechanisms on in vitro hydrogen peroxide (H2O2)-induced oxidative damage of osteoblasts and on in vivo lipopolysaccharide (LPS)-induced mice bone loss	516:678	This study was designed to investigate FLJC effects/mechanisms on in vitro hydrogen peroxide (H2O2)-induced oxidative damage of osteoblasts and on in vivo lipopolysaccharide (LPS)-induced mice bone loss.
31810119	13	139	theme	apoptosis	2303:2311	arg1	pathway					2313:2319	the mitochondrial apoptosis pathway	2285:2319	the mitochondrial apoptosis pathway	2285:2319	Additionally, FLJC protected against H2O2-induced apoptosis by inhibiting changes in expression of major Bcl-2 family effector proteins of the mitochondrial apoptosis pathway.
31810119	12	140	theme	osteoblast	2126:2135	arg1	markers					2137:2143	osteoblast markers	2126:2143	osteoblast markers	2126:2143	With regard to osteoblast differentiation, FLJC pretreatment increased ALP expression, as well as levels of mineralization and osteoblast markers.
31810119	6	141	theme	stem	911:914	arg1	differentiation					921:935	rat bone marrow mesenchymal stem cell differentiation	883:935	rat bone marrow mesenchymal stem cell differentiation induction	883:945	After rat bone marrow mesenchymal stem cell differentiation induction, resulting osteoblasts received various 48 h FLJC pretreatments before H2O2-based (200 μM) oxidative stress exposure.
31810119	2	142	theme	pharmacological	412:426	arg1	effect					428:433	its pharmacological effect	408:433	its pharmacological effect on osteoblasts	408:448	Fufang Lurong Jiangu Capsule (FLJC) has a known anti-osteoporotic effect, but its pharmacological effect on osteoblasts is not clearly understood.
31810119	14	143	theme	H2O2-induced	2361:2372	arg1	damage					2384:2389	H2O2-induced oxidative damage	2361:2389	H2O2-induced oxidative damage	2361:2389	Furthermore, FLJC protected cells from H2O2-induced oxidative damage by up-regulating Nrf2 and HO-1 protein levels.
31810119	3	144	theme	peroxide	561:568	arg1	damage					595:600	in vitro hydrogen peroxide (H2O2)-induced oxidative damage	543:600	in vitro hydrogen peroxide (H2O2)-induced oxidative damage of osteoblasts	543:615	This study was designed to investigate FLJC effects/mechanisms on in vitro hydrogen peroxide (H2O2)-induced oxidative damage of osteoblasts and on in vivo lipopolysaccharide (LPS)-induced mice bone loss.
30652080	0	0	theme	Candida	80:86	arg1	krusei					88:93	Candida krusei	80:93	Candida krusei	80:93	Presence of O-glycosidically linked oligosaccharides in the cell wall mannan of Candida krusei purified with Benanomicin A. Cell wall mannan of the pathogenic yeast Candida krusei was prepared using the antibiotic Benanomicin A, which has a lectin-like function.
30652080	0	1	theme	krusei	173:178	arg1	Presence					0:7	Presence	0:7	Presence of O-glycosidically linked oligosaccharides in the cell wall mannan of Candida krusei purified with Benanomicin A. Cell wall mannan of the pathogenic yeast Candida krusei	0:178	Presence of O-glycosidically linked oligosaccharides in the cell wall mannan of Candida krusei purified with Benanomicin A. Cell wall mannan of the pathogenic yeast Candida krusei was prepared using the antibiotic Benanomicin A, which has a lectin-like function.
30652080	4	2	theme	Benanomicin	1131:1141	arg1	A					1143:1143	Benanomicin A	1131:1143	Benanomicin A	1131:1143	In contrast, the O-glycosidic bond in mannan was not cleaved and the O-linked sugar chains were maintained and almost intact following treatment with the mild novel preparation method using Benanomicin A. Therefore, we argue that the new mannan preparation method using Benanomicin A is superior to conventional methods.
30652080	4	3	link	O-linked	930:937	arg1	chains					945:950	the O-linked sugar chains	926:950	the O-linked sugar chains	926:950	In contrast, the O-glycosidic bond in mannan was not cleaved and the O-linked sugar chains were maintained and almost intact following treatment with the mild novel preparation method using Benanomicin A. Therefore, we argue that the new mannan preparation method using Benanomicin A is superior to conventional methods.
30652080	0	4	theme	Candida	165:171	arg1	krusei					173:178	the pathogenic yeast Candida krusei	144:178	the pathogenic yeast Candida krusei	144:178	Presence of O-glycosidically linked oligosaccharides in the cell wall mannan of Candida krusei purified with Benanomicin A. Cell wall mannan of the pathogenic yeast Candida krusei was prepared using the antibiotic Benanomicin A, which has a lectin-like function.
30652080	0	5	link	linked	29:34	arg1	oligosaccharides					36:51	O-glycosidically linked oligosaccharides	12:51	O-glycosidically linked oligosaccharides in the cell wall mannan of Candida krusei purified with Benanomicin A. Cell wall mannan	12:139	Presence of O-glycosidically linked oligosaccharides in the cell wall mannan of Candida krusei purified with Benanomicin A. Cell wall mannan of the pathogenic yeast Candida krusei was prepared using the antibiotic Benanomicin A, which has a lectin-like function.
30652080	4	6	from	bond	891:894	arg1	mannan					899:904	mannan	899:904	mannan	899:904	In contrast, the O-glycosidic bond in mannan was not cleaved and the O-linked sugar chains were maintained and almost intact following treatment with the mild novel preparation method using Benanomicin A. Therefore, we argue that the new mannan preparation method using Benanomicin A is superior to conventional methods.
30652080	3	7	theme	Fehling	844:850	arg1	reagent					852:858	the strongly alkaline Fehling reagent	822:858	the strongly alkaline Fehling reagent	822:858	These results indicate that most of the O-linked sugar chains in mannan were lost under conventional conditions when exposed to the strongly alkaline Fehling reagent.
30652080	0	8	from	Presence	0:7	arg1	mannan					70:75	the cell wall mannan	56:75	the cell wall mannan of Candida krusei	56:93	Presence of O-glycosidically linked oligosaccharides in the cell wall mannan of Candida krusei purified with Benanomicin A. Cell wall mannan of the pathogenic yeast Candida krusei was prepared using the antibiotic Benanomicin A, which has a lectin-like function.
30652080	3	9	from	chains	749:754	arg1	mannan					759:764	mannan	759:764	mannan	759:764	These results indicate that most of the O-linked sugar chains in mannan were lost under conventional conditions when exposed to the strongly alkaline Fehling reagent.
30652080	1	10	theme	Fehling	407:413	arg1	reagent					415:421	Fehling reagent	407:421	Fehling reagent	407:421	The chemical structure of this molecule was found to be similar to that of mannan prepared from the same yeast by the conventional method using Fehling reagent.
30652080	4	11	theme	preparation	1026:1036	arg1	method					1038:1043	the mild novel preparation method	1011:1043	the mild novel preparation method using Benanomicin A. Therefore, we argue that the new mannan preparation method using Benanomicin A is superior to conventional methods	1011:1179	In contrast, the O-glycosidic bond in mannan was not cleaved and the O-linked sugar chains were maintained and almost intact following treatment with the mild novel preparation method using Benanomicin A. Therefore, we argue that the new mannan preparation method using Benanomicin A is superior to conventional methods.
30652080	3	12	theme	conventional	782:793	arg1	conditions					795:804	conventional conditions	782:804	conventional conditions	782:804	These results indicate that most of the O-linked sugar chains in mannan were lost under conventional conditions when exposed to the strongly alkaline Fehling reagent.
30652080	0	13	theme	krusei	88:93	arg1	mannan					70:75	the cell wall mannan	56:75	the cell wall mannan of Candida krusei	56:93	Presence of O-glycosidically linked oligosaccharides in the cell wall mannan of Candida krusei purified with Benanomicin A. Cell wall mannan of the pathogenic yeast Candida krusei was prepared using the antibiotic Benanomicin A, which has a lectin-like function.
30652080	4	14	with	treatment	996:1004	arg1	method					1038:1043	the mild novel preparation method	1011:1043	the mild novel preparation method using Benanomicin A. Therefore, we argue that the new mannan preparation method using Benanomicin A is superior to conventional methods	1011:1179	In contrast, the O-glycosidic bond in mannan was not cleaved and the O-linked sugar chains were maintained and almost intact following treatment with the mild novel preparation method using Benanomicin A. Therefore, we argue that the new mannan preparation method using Benanomicin A is superior to conventional methods.
30652080	5	15	contain	contain	1294:1300	arg1	mannans					1230:1236	some yeast mannans	1219:1236	some yeast mannans	1219:1236	In addition, our study suggests that some yeast mannans, whose overall structure has already been reported, may contain more O-linked sugar chains than previously recognized.
30652080	5	15	contain	contain	1294:1300	arg2	chains					1322:1327	more O-linked sugar chains	1302:1327	more O-linked sugar chains	1302:1327	In addition, our study suggests that some yeast mannans, whose overall structure has already been reported, may contain more O-linked sugar chains than previously recognized.
30652080	4	16	theme	new	1095:1097	arg1	superior					1148:1155	superior	1148:1155	superior	1148:1155	In contrast, the O-glycosidic bond in mannan was not cleaved and the O-linked sugar chains were maintained and almost intact following treatment with the mild novel preparation method using Benanomicin A. Therefore, we argue that the new mannan preparation method using Benanomicin A is superior to conventional methods.
30652080	4	16	theme	new	1095:1097	arg1	method					1118:1123	the new mannan preparation method	1091:1123	the new mannan preparation method using Benanomicin A	1091:1143	In contrast, the O-glycosidic bond in mannan was not cleaved and the O-linked sugar chains were maintained and almost intact following treatment with the mild novel preparation method using Benanomicin A. Therefore, we argue that the new mannan preparation method using Benanomicin A is superior to conventional methods.
30652080	4	17	theme	mannan	1099:1104	arg1	superior					1148:1155	superior	1148:1155	superior	1148:1155	In contrast, the O-glycosidic bond in mannan was not cleaved and the O-linked sugar chains were maintained and almost intact following treatment with the mild novel preparation method using Benanomicin A. Therefore, we argue that the new mannan preparation method using Benanomicin A is superior to conventional methods.
30652080	4	17	theme	mannan	1099:1104	arg1	method					1118:1123	the new mannan preparation method	1091:1123	the new mannan preparation method using Benanomicin A	1091:1143	In contrast, the O-glycosidic bond in mannan was not cleaved and the O-linked sugar chains were maintained and almost intact following treatment with the mild novel preparation method using Benanomicin A. Therefore, we argue that the new mannan preparation method using Benanomicin A is superior to conventional methods.
30652080	0	18	theme	Benanomicin	109:119	arg1	mannan					134:139	Benanomicin A. Cell wall mannan	109:139	Benanomicin A. Cell wall mannan	109:139	Presence of O-glycosidically linked oligosaccharides in the cell wall mannan of Candida krusei purified with Benanomicin A. Cell wall mannan of the pathogenic yeast Candida krusei was prepared using the antibiotic Benanomicin A, which has a lectin-like function.
30652080	4	19	theme	mild	1015:1018	arg1	method					1038:1043	the mild novel preparation method	1011:1043	the mild novel preparation method using Benanomicin A. Therefore, we argue that the new mannan preparation method using Benanomicin A is superior to conventional methods	1011:1179	In contrast, the O-glycosidic bond in mannan was not cleaved and the O-linked sugar chains were maintained and almost intact following treatment with the mild novel preparation method using Benanomicin A. Therefore, we argue that the new mannan preparation method using Benanomicin A is superior to conventional methods.
30652080	0	20	theme	lectin-like	241:251	arg1	function					253:260	a lectin-like function	239:260	a lectin-like function	239:260	Presence of O-glycosidically linked oligosaccharides in the cell wall mannan of Candida krusei purified with Benanomicin A. Cell wall mannan of the pathogenic yeast Candida krusei was prepared using the antibiotic Benanomicin A, which has a lectin-like function.
30652080	4	21	theme	preparation	1106:1116	arg1	superior					1148:1155	superior	1148:1155	superior	1148:1155	In contrast, the O-glycosidic bond in mannan was not cleaved and the O-linked sugar chains were maintained and almost intact following treatment with the mild novel preparation method using Benanomicin A. Therefore, we argue that the new mannan preparation method using Benanomicin A is superior to conventional methods.
30652080	4	21	theme	preparation	1106:1116	arg1	method					1118:1123	the new mannan preparation method	1091:1123	the new mannan preparation method using Benanomicin A	1091:1143	In contrast, the O-glycosidic bond in mannan was not cleaved and the O-linked sugar chains were maintained and almost intact following treatment with the mild novel preparation method using Benanomicin A. Therefore, we argue that the new mannan preparation method using Benanomicin A is superior to conventional methods.
30652080	0	22	from	oligosaccharides	36:51	arg1	mannan					70:75	the cell wall mannan	56:75	the cell wall mannan of Candida krusei	56:93	Presence of O-glycosidically linked oligosaccharides in the cell wall mannan of Candida krusei purified with Benanomicin A. Cell wall mannan of the pathogenic yeast Candida krusei was prepared using the antibiotic Benanomicin A, which has a lectin-like function.
30652080	0	23	theme	Benanomicin	214:224	arg1	A					226:226	the antibiotic Benanomicin A	199:226	the antibiotic Benanomicin A	199:226	Presence of O-glycosidically linked oligosaccharides in the cell wall mannan of Candida krusei purified with Benanomicin A. Cell wall mannan of the pathogenic yeast Candida krusei was prepared using the antibiotic Benanomicin A, which has a lectin-like function.
30652080	2	24	theme	Benanomicin	655:665	arg1	A					667:667	Benanomicin A	655:667	Benanomicin A	655:667	Only a few degradation products were detected when the mannan prepared using Fehling reagent was subjected to alkali treatment (β-elimination), but multiple α-1,2-linked oligosaccharides were detected when the mannan purified with Benanomicin A was treated with alkali.
30652080	2	25	theme	α-1,2-linked	581:592	arg1	oligosaccharides					594:609	multiple α-1,2-linked oligosaccharides	572:609	multiple α-1,2-linked oligosaccharides	572:609	Only a few degradation products were detected when the mannan prepared using Fehling reagent was subjected to alkali treatment (β-elimination), but multiple α-1,2-linked oligosaccharides were detected when the mannan purified with Benanomicin A was treated with alkali.
30652080	0	26	theme	antibiotic	203:212	arg1	A					226:226	the antibiotic Benanomicin A	199:226	the antibiotic Benanomicin A	199:226	Presence of O-glycosidically linked oligosaccharides in the cell wall mannan of Candida krusei purified with Benanomicin A. Cell wall mannan of the pathogenic yeast Candida krusei was prepared using the antibiotic Benanomicin A, which has a lectin-like function.
30652080	4	27	dep	Benanomicin	1051:1061	arg1	argue					1080:1084	argue	1080:1084	argue that the new mannan preparation method using Benanomicin A is superior to conventional methods	1080:1179	In contrast, the O-glycosidic bond in mannan was not cleaved and the O-linked sugar chains were maintained and almost intact following treatment with the mild novel preparation method using Benanomicin A. Therefore, we argue that the new mannan preparation method using Benanomicin A is superior to conventional methods.
30652080	4	27	dep	Benanomicin	1051:1061	arg1	A.					1063:1064	Benanomicin A. Therefore, we argue that the new mannan preparation method using Benanomicin A is superior to conventional methods	1051:1179	Benanomicin A. Therefore, we argue that the new mannan preparation method using Benanomicin A is superior to conventional methods	1051:1179	In contrast, the O-glycosidic bond in mannan was not cleaved and the O-linked sugar chains were maintained and almost intact following treatment with the mild novel preparation method using Benanomicin A. Therefore, we argue that the new mannan preparation method using Benanomicin A is superior to conventional methods.
30652080	0	28	theme	linked	29:34	arg1	oligosaccharides					36:51	O-glycosidically linked oligosaccharides	12:51	O-glycosidically linked oligosaccharides in the cell wall mannan of Candida krusei purified with Benanomicin A. Cell wall mannan	12:139	Presence of O-glycosidically linked oligosaccharides in the cell wall mannan of Candida krusei purified with Benanomicin A. Cell wall mannan of the pathogenic yeast Candida krusei was prepared using the antibiotic Benanomicin A, which has a lectin-like function.
30652080	1	29	theme	chemical	267:274	arg1	structure					276:284	The chemical structure	263:284	The chemical structure of this molecule	263:301	The chemical structure of this molecule was found to be similar to that of mannan prepared from the same yeast by the conventional method using Fehling reagent.
30652080	1	29	theme	chemical	267:274	arg1	similar					319:325	similar	319:325	similar	319:325	The chemical structure of this molecule was found to be similar to that of mannan prepared from the same yeast by the conventional method using Fehling reagent.
30652080	0	30	theme	Cell	124:127	arg1	mannan					134:139	Benanomicin A. Cell wall mannan	109:139	Benanomicin A. Cell wall mannan	109:139	Presence of O-glycosidically linked oligosaccharides in the cell wall mannan of Candida krusei purified with Benanomicin A. Cell wall mannan of the pathogenic yeast Candida krusei was prepared using the antibiotic Benanomicin A, which has a lectin-like function.
30652080	5	31	theme	sugar	1316:1320	arg1	chains					1322:1327	more O-linked sugar chains	1302:1327	more O-linked sugar chains	1302:1327	In addition, our study suggests that some yeast mannans, whose overall structure has already been reported, may contain more O-linked sugar chains than previously recognized.
30652080	5	32	link	O-linked	1307:1314	arg1	chains					1322:1327	more O-linked sugar chains	1302:1327	more O-linked sugar chains	1302:1327	In addition, our study suggests that some yeast mannans, whose overall structure has already been reported, may contain more O-linked sugar chains than previously recognized.
30652080	0	33	theme	A.	121:122	arg1	mannan					134:139	Benanomicin A. Cell wall mannan	109:139	Benanomicin A. Cell wall mannan	109:139	Presence of O-glycosidically linked oligosaccharides in the cell wall mannan of Candida krusei purified with Benanomicin A. Cell wall mannan of the pathogenic yeast Candida krusei was prepared using the antibiotic Benanomicin A, which has a lectin-like function.
30652080	2	34	theme	degradation	435:445	arg1	products					447:454	Only a few degradation products	424:454	Only a few degradation products	424:454	Only a few degradation products were detected when the mannan prepared using Fehling reagent was subjected to alkali treatment (β-elimination), but multiple α-1,2-linked oligosaccharides were detected when the mannan purified with Benanomicin A was treated with alkali.
30652080	5	35	theme	yeast	1224:1228	arg1	mannans					1230:1236	some yeast mannans	1219:1236	some yeast mannans	1219:1236	In addition, our study suggests that some yeast mannans, whose overall structure has already been reported, may contain more O-linked sugar chains than previously recognized.
30652080	0	36	theme	oligosaccharides	36:51	arg1	Presence					0:7	Presence	0:7	Presence of O-glycosidically linked oligosaccharides in the cell wall mannan of Candida krusei purified with Benanomicin A. Cell wall mannan of the pathogenic yeast Candida krusei	0:178	Presence of O-glycosidically linked oligosaccharides in the cell wall mannan of Candida krusei purified with Benanomicin A. Cell wall mannan of the pathogenic yeast Candida krusei was prepared using the antibiotic Benanomicin A, which has a lectin-like function.
30652080	0	37	contain	has	235:237	arg1	A					226:226	the antibiotic Benanomicin A	199:226	the antibiotic Benanomicin A	199:226	Presence of O-glycosidically linked oligosaccharides in the cell wall mannan of Candida krusei purified with Benanomicin A. Cell wall mannan of the pathogenic yeast Candida krusei was prepared using the antibiotic Benanomicin A, which has a lectin-like function.
30652080	0	37	contain	has	235:237	arg2	function					253:260	a lectin-like function	239:260	a lectin-like function	239:260	Presence of O-glycosidically linked oligosaccharides in the cell wall mannan of Candida krusei purified with Benanomicin A. Cell wall mannan of the pathogenic yeast Candida krusei was prepared using the antibiotic Benanomicin A, which has a lectin-like function.
30652080	0	38	from	mannan	70:75	arg1	Presence					0:7	Presence	0:7	Presence of O-glycosidically linked oligosaccharides in the cell wall mannan of Candida krusei purified with Benanomicin A. Cell wall mannan of the pathogenic yeast Candida krusei	0:178	Presence of O-glycosidically linked oligosaccharides in the cell wall mannan of Candida krusei purified with Benanomicin A. Cell wall mannan of the pathogenic yeast Candida krusei was prepared using the antibiotic Benanomicin A, which has a lectin-like function.
30652080	2	39	theme	Fehling	501:507	arg1	reagent					509:515	Fehling reagent	501:515	Fehling reagent	501:515	Only a few degradation products were detected when the mannan prepared using Fehling reagent was subjected to alkali treatment (β-elimination), but multiple α-1,2-linked oligosaccharides were detected when the mannan purified with Benanomicin A was treated with alkali.
30652080	0	40	theme	cell	60:63	arg1	mannan					70:75	the cell wall mannan	56:75	the cell wall mannan of Candida krusei	56:93	Presence of O-glycosidically linked oligosaccharides in the cell wall mannan of Candida krusei purified with Benanomicin A. Cell wall mannan of the pathogenic yeast Candida krusei was prepared using the antibiotic Benanomicin A, which has a lectin-like function.
30652080	1	41	theme	molecule	294:301	arg1	structure					276:284	The chemical structure	263:284	The chemical structure of this molecule	263:301	The chemical structure of this molecule was found to be similar to that of mannan prepared from the same yeast by the conventional method using Fehling reagent.
30652080	1	41	theme	molecule	294:301	arg1	similar					319:325	similar	319:325	similar	319:325	The chemical structure of this molecule was found to be similar to that of mannan prepared from the same yeast by the conventional method using Fehling reagent.
30652080	4	42	theme	O-linked	930:937	arg1	chains					945:950	the O-linked sugar chains	926:950	the O-linked sugar chains	926:950	In contrast, the O-glycosidic bond in mannan was not cleaved and the O-linked sugar chains were maintained and almost intact following treatment with the mild novel preparation method using Benanomicin A. Therefore, we argue that the new mannan preparation method using Benanomicin A is superior to conventional methods.
30652080	2	43	theme	alkali	534:539	arg1	β-elimination					552:564	β-elimination	552:564	β-elimination	552:564	Only a few degradation products were detected when the mannan prepared using Fehling reagent was subjected to alkali treatment (β-elimination), but multiple α-1,2-linked oligosaccharides were detected when the mannan purified with Benanomicin A was treated with alkali.
30652080	2	43	theme	alkali	534:539	arg1	treatment					541:549	alkali treatment	534:549	alkali treatment (β-elimination)	534:565	Only a few degradation products were detected when the mannan prepared using Fehling reagent was subjected to alkali treatment (β-elimination), but multiple α-1,2-linked oligosaccharides were detected when the mannan purified with Benanomicin A was treated with alkali.
30652080	0	44	theme	wall	129:132	arg1	mannan					134:139	Benanomicin A. Cell wall mannan	109:139	Benanomicin A. Cell wall mannan	109:139	Presence of O-glycosidically linked oligosaccharides in the cell wall mannan of Candida krusei purified with Benanomicin A. Cell wall mannan of the pathogenic yeast Candida krusei was prepared using the antibiotic Benanomicin A, which has a lectin-like function.
30652080	3	45	theme	O-linked	734:741	arg1	chains					749:754	the O-linked sugar chains	730:754	the O-linked sugar chains in mannan	730:764	These results indicate that most of the O-linked sugar chains in mannan were lost under conventional conditions when exposed to the strongly alkaline Fehling reagent.
30652080	4	46	theme	conventional	1160:1171	arg1	methods					1173:1179	conventional methods	1160:1179	conventional methods	1160:1179	In contrast, the O-glycosidic bond in mannan was not cleaved and the O-linked sugar chains were maintained and almost intact following treatment with the mild novel preparation method using Benanomicin A. Therefore, we argue that the new mannan preparation method using Benanomicin A is superior to conventional methods.
30652080	1	47	theme	same	363:366	arg1	yeast					368:372	the same yeast	359:372	the same yeast	359:372	The chemical structure of this molecule was found to be similar to that of mannan prepared from the same yeast by the conventional method using Fehling reagent.
30652080	3	48	theme	sugar	743:747	arg1	chains					749:754	the O-linked sugar chains	730:754	the O-linked sugar chains in mannan	730:764	These results indicate that most of the O-linked sugar chains in mannan were lost under conventional conditions when exposed to the strongly alkaline Fehling reagent.
30652080	0	49	theme	yeast	159:163	arg1	krusei					173:178	the pathogenic yeast Candida krusei	144:178	the pathogenic yeast Candida krusei	144:178	Presence of O-glycosidically linked oligosaccharides in the cell wall mannan of Candida krusei purified with Benanomicin A. Cell wall mannan of the pathogenic yeast Candida krusei was prepared using the antibiotic Benanomicin A, which has a lectin-like function.
30652080	2	50	theme	multiple	572:579	arg1	oligosaccharides					594:609	multiple α-1,2-linked oligosaccharides	572:609	multiple α-1,2-linked oligosaccharides	572:609	Only a few degradation products were detected when the mannan prepared using Fehling reagent was subjected to alkali treatment (β-elimination), but multiple α-1,2-linked oligosaccharides were detected when the mannan purified with Benanomicin A was treated with alkali.
30652080	0	51	theme	pathogenic	148:157	arg1	krusei					173:178	the pathogenic yeast Candida krusei	144:178	the pathogenic yeast Candida krusei	144:178	Presence of O-glycosidically linked oligosaccharides in the cell wall mannan of Candida krusei purified with Benanomicin A. Cell wall mannan of the pathogenic yeast Candida krusei was prepared using the antibiotic Benanomicin A, which has a lectin-like function.
30652080	1	52	theme	conventional	381:392	arg1	method					394:399	the conventional method	377:399	the conventional method using Fehling reagent	377:421	The chemical structure of this molecule was found to be similar to that of mannan prepared from the same yeast by the conventional method using Fehling reagent.
30652080	3	53	link	O-linked	734:741	arg1	chains					749:754	the O-linked sugar chains	730:754	the O-linked sugar chains in mannan	730:764	These results indicate that most of the O-linked sugar chains in mannan were lost under conventional conditions when exposed to the strongly alkaline Fehling reagent.
30652080	5	54	theme	O-linked	1307:1314	arg1	chains					1322:1327	more O-linked sugar chains	1302:1327	more O-linked sugar chains	1302:1327	In addition, our study suggests that some yeast mannans, whose overall structure has already been reported, may contain more O-linked sugar chains than previously recognized.
30652080	5	55	theme	overall	1245:1251	arg1	structure					1253:1261	structure	1253:1261	structure	1253:1261	In addition, our study suggests that some yeast mannans, whose overall structure has already been reported, may contain more O-linked sugar chains than previously recognized.
30652080	4	56	theme	sugar	939:943	arg1	chains					945:950	the O-linked sugar chains	926:950	the O-linked sugar chains	926:950	In contrast, the O-glycosidic bond in mannan was not cleaved and the O-linked sugar chains were maintained and almost intact following treatment with the mild novel preparation method using Benanomicin A. Therefore, we argue that the new mannan preparation method using Benanomicin A is superior to conventional methods.
30652080	4	57	theme	novel	1020:1024	arg1	method					1038:1043	the mild novel preparation method	1011:1043	the mild novel preparation method using Benanomicin A. Therefore, we argue that the new mannan preparation method using Benanomicin A is superior to conventional methods	1011:1179	In contrast, the O-glycosidic bond in mannan was not cleaved and the O-linked sugar chains were maintained and almost intact following treatment with the mild novel preparation method using Benanomicin A. Therefore, we argue that the new mannan preparation method using Benanomicin A is superior to conventional methods.
30652080	4	58	theme	O-glycosidic	878:889	arg1	bond					891:894	the O-glycosidic bond	874:894	the O-glycosidic bond in mannan	874:904	In contrast, the O-glycosidic bond in mannan was not cleaved and the O-linked sugar chains were maintained and almost intact following treatment with the mild novel preparation method using Benanomicin A. Therefore, we argue that the new mannan preparation method using Benanomicin A is superior to conventional methods.
30652080	0	59	theme	wall	65:68	arg1	mannan					70:75	the cell wall mannan	56:75	the cell wall mannan of Candida krusei	56:93	Presence of O-glycosidically linked oligosaccharides in the cell wall mannan of Candida krusei purified with Benanomicin A. Cell wall mannan of the pathogenic yeast Candida krusei was prepared using the antibiotic Benanomicin A, which has a lectin-like function.
30652080	2	60	link	α-1,2-linked	581:592	arg1	oligosaccharides					594:609	multiple α-1,2-linked oligosaccharides	572:609	multiple α-1,2-linked oligosaccharides	572:609	Only a few degradation products were detected when the mannan prepared using Fehling reagent was subjected to alkali treatment (β-elimination), but multiple α-1,2-linked oligosaccharides were detected when the mannan purified with Benanomicin A was treated with alkali.
29382534	5	0	theme	human	732:736	arg1	cells					749:753	human epithelial cells	732:753	human epithelial cells	732:753	In this study, we investigated the anti-asthmatic activity and mechanism of SalB in a murine model and human epithelial cells by monitoring changes in mucin expression and secretion, airway inflammation, AHR, and signaling pathways.
29382534	5	1	from	changes	769:775	arg1	secretion					801:809	secretion	801:809	secretion	801:809	In this study, we investigated the anti-asthmatic activity and mechanism of SalB in a murine model and human epithelial cells by monitoring changes in mucin expression and secretion, airway inflammation, AHR, and signaling pathways.
29382534	5	1	from	changes	769:775	arg1	expression					786:795	mucin expression	780:795	mucin expression	780:795	In this study, we investigated the anti-asthmatic activity and mechanism of SalB in a murine model and human epithelial cells by monitoring changes in mucin expression and secretion, airway inflammation, AHR, and signaling pathways.
29382534	11	2	theme	IL-13-induced	1630:1642	arg1	expression					1678:1687	IL-13-induced MUC5AC and MUC5B mRNA and protein expression	1630:1687	IL-13-induced MUC5AC and MUC5B mRNA and protein expression in human epithelial cells	1630:1713	In vitro, SalB significantly inhibited IL-13-induced MUC5AC and MUC5B mRNA and protein expression in human epithelial cells.
29382534	11	3	theme	MUC5AC	1644:1649	arg1	mRNA					1661:1664	MUC5AC and MUC5B mRNA	1644:1664	MUC5AC and MUC5B mRNA	1644:1664	In vitro, SalB significantly inhibited IL-13-induced MUC5AC and MUC5B mRNA and protein expression in human epithelial cells.
29382534	4	4	theme	inflammation	558:569	arg1	Overproduction					501:514	Overproduction	501:514	Overproduction of airway MUC5AC	501:531	Overproduction of airway MUC5AC is a central effector of inflammation that is strongly associated with AHR in asthmatic attack.
29382534	4	4	theme	inflammation	558:569	arg1	effector					546:553	a central effector	536:553	a central effector of inflammation that is strongly associated with AHR in asthmatic attack	536:626	Overproduction of airway MUC5AC is a central effector of inflammation that is strongly associated with AHR in asthmatic attack.
29382534	5	5	theme	epithelial	738:747	arg1	cells					749:753	human epithelial cells	732:753	human epithelial cells	732:753	In this study, we investigated the anti-asthmatic activity and mechanism of SalB in a murine model and human epithelial cells by monitoring changes in mucin expression and secretion, airway inflammation, AHR, and signaling pathways.
29382534	13	6	with	treatment	1849:1857	arg1	SalB					1864:1867	SalB	1864:1867	SalB	1864:1867	Taken together, these data indicate that treatment with SalB may improve AHR by inhibiting MUC5AC overproduction.
29382534	8	7	with	treatments	1083:1092	arg1	SalB					1099:1102	SalB	1099:1102	SalB	1099:1102	We found that treatments with SalB significantly inhibited increase in the tracheobronchial secretion, glycosaminoglycan levels, interleukin (IL)-13, IL-4, and IL-5 cytokines mRNA and protein expression, and decrease in mucociliary clearance in lung tissues.
29382534	8	8	theme	protein	1253:1259	arg1	levels					1190:1195	glycosaminoglycan levels	1172:1195	glycosaminoglycan levels	1172:1195	We found that treatments with SalB significantly inhibited increase in the tracheobronchial secretion, glycosaminoglycan levels, interleukin (IL)-13, IL-4, and IL-5 cytokines mRNA and protein expression, and decrease in mucociliary clearance in lung tissues.
29382534	8	8	theme	protein	1253:1259	arg1	expression					1261:1270	protein expression	1253:1270	protein expression	1253:1270	We found that treatments with SalB significantly inhibited increase in the tracheobronchial secretion, glycosaminoglycan levels, interleukin (IL)-13, IL-4, and IL-5 cytokines mRNA and protein expression, and decrease in mucociliary clearance in lung tissues.
29382534	5	9	theme	SalB	705:708	arg1	mechanism					692:700	mechanism	692:700	mechanism	692:700	In this study, we investigated the anti-asthmatic activity and mechanism of SalB in a murine model and human epithelial cells by monitoring changes in mucin expression and secretion, airway inflammation, AHR, and signaling pathways.
29382534	5	9	theme	SalB	705:708	arg1	activity					679:686	the anti-asthmatic activity	660:686	the anti-asthmatic activity	660:686	In this study, we investigated the anti-asthmatic activity and mechanism of SalB in a murine model and human epithelial cells by monitoring changes in mucin expression and secretion, airway inflammation, AHR, and signaling pathways.
29382534	8	10	from	increase	1128:1135	arg1	clearance					1301:1309	mucociliary clearance	1289:1309	mucociliary clearance	1289:1309	We found that treatments with SalB significantly inhibited increase in the tracheobronchial secretion, glycosaminoglycan levels, interleukin (IL)-13, IL-4, and IL-5 cytokines mRNA and protein expression, and decrease in mucociliary clearance in lung tissues.
29382534	8	10	from	increase	1128:1135	arg1	tissues					1319:1325	lung tissues	1314:1325	lung tissues	1314:1325	We found that treatments with SalB significantly inhibited increase in the tracheobronchial secretion, glycosaminoglycan levels, interleukin (IL)-13, IL-4, and IL-5 cytokines mRNA and protein expression, and decrease in mucociliary clearance in lung tissues.
29382534	8	10	from	increase	1128:1135	arg1	secretion					1161:1169	the tracheobronchial secretion	1140:1169	the tracheobronchial secretion	1140:1169	We found that treatments with SalB significantly inhibited increase in the tracheobronchial secretion, glycosaminoglycan levels, interleukin (IL)-13, IL-4, and IL-5 cytokines mRNA and protein expression, and decrease in mucociliary clearance in lung tissues.
29382534	7	11	theme	last	1045:1048	arg1	challenge					1058:1066	the last antigen challenge	1041:1066	the last antigen challenge	1041:1066	All examinations were performed 24h after the last antigen challenge.
29382534	10	12	theme	protective	1533:1542	arg1	effects					1544:1550	protective effects	1533:1550	protective effects against AHR in OVA-challenged animals	1533:1588	SalB exhibited protective effects against AHR in OVA-challenged animals.
29382534	2	13	theme	various	287:293	arg1	diseases					295:302	various diseases	287:302	various diseases including cardiovascular, lung, Liver, renal and cancers	287:359	It is used for clinical treatment of various diseases including cardiovascular, lung, Liver, renal and cancers.
29382534	2	13	theme	various	287:293	arg1	cancers					353:359	cancers	353:359	cancers	353:359	It is used for clinical treatment of various diseases including cardiovascular, lung, Liver, renal and cancers.
29382534	2	13	theme	various	287:293	arg1	Liver					336:340	Liver	336:340	Liver	336:340	It is used for clinical treatment of various diseases including cardiovascular, lung, Liver, renal and cancers.
29382534	2	13	theme	various	287:293	arg1	lung					330:333	lung	330:333	lung	330:333	It is used for clinical treatment of various diseases including cardiovascular, lung, Liver, renal and cancers.
29382534	2	13	theme	various	287:293	arg1	renal					343:347	renal	343:347	renal	343:347	It is used for clinical treatment of various diseases including cardiovascular, lung, Liver, renal and cancers.
29382534	5	14	from	activity	679:686	arg1	cells					749:753	human epithelial cells	732:753	human epithelial cells	732:753	In this study, we investigated the anti-asthmatic activity and mechanism of SalB in a murine model and human epithelial cells by monitoring changes in mucin expression and secretion, airway inflammation, AHR, and signaling pathways.
29382534	5	14	from	activity	679:686	arg1	model					722:726	a murine model	713:726	a murine model	713:726	In this study, we investigated the anti-asthmatic activity and mechanism of SalB in a murine model and human epithelial cells by monitoring changes in mucin expression and secretion, airway inflammation, AHR, and signaling pathways.
29382534	0	15	theme	Erk1/2/P38	107:116	arg1	signaling					118:126	Erk1/2/P38 signaling	107:126	Erk1/2/P38 signaling	107:126	Salvianolic acid B improves airway hyperresponsiveness by inhibiting MUC5AC overproduction associated with Erk1/2/P38 signaling.
29382534	8	16	from	levels	1190:1195	arg1	clearance					1301:1309	mucociliary clearance	1289:1309	mucociliary clearance	1289:1309	We found that treatments with SalB significantly inhibited increase in the tracheobronchial secretion, glycosaminoglycan levels, interleukin (IL)-13, IL-4, and IL-5 cytokines mRNA and protein expression, and decrease in mucociliary clearance in lung tissues.
29382534	8	16	from	levels	1190:1195	arg1	tissues					1319:1325	lung tissues	1314:1325	lung tissues	1314:1325	We found that treatments with SalB significantly inhibited increase in the tracheobronchial secretion, glycosaminoglycan levels, interleukin (IL)-13, IL-4, and IL-5 cytokines mRNA and protein expression, and decrease in mucociliary clearance in lung tissues.
29382534	8	16	from	levels	1190:1195	arg1	secretion					1161:1169	the tracheobronchial secretion	1140:1169	the tracheobronchial secretion	1140:1169	We found that treatments with SalB significantly inhibited increase in the tracheobronchial secretion, glycosaminoglycan levels, interleukin (IL)-13, IL-4, and IL-5 cytokines mRNA and protein expression, and decrease in mucociliary clearance in lung tissues.
29382534	7	17	theme	antigen	1050:1056	arg1	challenge					1058:1066	the last antigen challenge	1041:1066	the last antigen challenge	1041:1066	All examinations were performed 24h after the last antigen challenge.
29382534	9	18	theme	goblet	1427:1432	arg1	hyperplasia					1439:1449	airway goblet cell hyperplasia	1420:1449	airway goblet cell hyperplasia	1420:1449	Histological results demonstrated that SalB attenuated OVA-induced eosinophil infiltration, airway goblet cell hyperplasia, and MUC5AC and MUC5B mRNA and protein expression in lung tissues.
29382534	2	19	theme	clinical	265:272	arg1	treatment					274:282	clinical treatment	265:282	clinical treatment of various diseases including cardiovascular, lung, Liver, renal and cancers	265:359	It is used for clinical treatment of various diseases including cardiovascular, lung, Liver, renal and cancers.
29382534	8	20	theme	IL	1211:1212	arg1	mRNA					1244:1247	interleukin (IL)-13, IL-4, and IL-5 cytokines mRNA	1198:1247	interleukin (IL)-13, IL-4, and IL-5 cytokines mRNA	1198:1247	We found that treatments with SalB significantly inhibited increase in the tracheobronchial secretion, glycosaminoglycan levels, interleukin (IL)-13, IL-4, and IL-5 cytokines mRNA and protein expression, and decrease in mucociliary clearance in lung tissues.
29382534	8	20	theme	IL	1211:1212	arg1	levels					1190:1195	glycosaminoglycan levels	1172:1195	glycosaminoglycan levels	1172:1195	We found that treatments with SalB significantly inhibited increase in the tracheobronchial secretion, glycosaminoglycan levels, interleukin (IL)-13, IL-4, and IL-5 cytokines mRNA and protein expression, and decrease in mucociliary clearance in lung tissues.
29382534	3	21	theme	airway	410:415	arg1	hypersecretion					423:436	allergy induced airway mucin hypersecretion	394:436	allergy induced airway mucin hypersecretion	394:436	However, the effects of SalB to allergy induced airway mucin hypersecretion, inflammation and hyperresponsiveness (AHR) remains not clear.
29382534	5	22	theme	murine	715:720	arg1	model					722:726	a murine model	713:726	a murine model	713:726	In this study, we investigated the anti-asthmatic activity and mechanism of SalB in a murine model and human epithelial cells by monitoring changes in mucin expression and secretion, airway inflammation, AHR, and signaling pathways.
29382534	5	23	from	mechanism	692:700	arg1	cells					749:753	human epithelial cells	732:753	human epithelial cells	732:753	In this study, we investigated the anti-asthmatic activity and mechanism of SalB in a murine model and human epithelial cells by monitoring changes in mucin expression and secretion, airway inflammation, AHR, and signaling pathways.
29382534	5	23	from	mechanism	692:700	arg1	model					722:726	a murine model	713:726	a murine model	713:726	In this study, we investigated the anti-asthmatic activity and mechanism of SalB in a murine model and human epithelial cells by monitoring changes in mucin expression and secretion, airway inflammation, AHR, and signaling pathways.
29382534	13	24	theme	MUC5AC	1899:1904	arg1	overproduction					1906:1919	MUC5AC overproduction	1899:1919	MUC5AC overproduction	1899:1919	Taken together, these data indicate that treatment with SalB may improve AHR by inhibiting MUC5AC overproduction.
29382534	5	25	theme	mucin	780:784	arg1	expression					786:795	mucin expression	780:795	mucin expression	780:795	In this study, we investigated the anti-asthmatic activity and mechanism of SalB in a murine model and human epithelial cells by monitoring changes in mucin expression and secretion, airway inflammation, AHR, and signaling pathways.
29382534	1	26	theme	main	169:172	arg1	composites					188:197	the main water-soluble composites	165:197	the main water-soluble composites from Chinese medicine Dansen (Radix miltiorrhiza)	165:247	Salvianolic acid B (SalB) is one of the main water-soluble composites from Chinese medicine Dansen (Radix miltiorrhiza).
29382534	0	27	theme	acid	12:15	arg1	B					17:17	Salvianolic acid B	0:17	Salvianolic acid B	0:17	Salvianolic acid B improves airway hyperresponsiveness by inhibiting MUC5AC overproduction associated with Erk1/2/P38 signaling.
29382534	8	28	from	decrease	1277:1284	arg1	clearance					1301:1309	mucociliary clearance	1289:1309	mucociliary clearance	1289:1309	We found that treatments with SalB significantly inhibited increase in the tracheobronchial secretion, glycosaminoglycan levels, interleukin (IL)-13, IL-4, and IL-5 cytokines mRNA and protein expression, and decrease in mucociliary clearance in lung tissues.
29382534	8	28	from	decrease	1277:1284	arg1	tissues					1319:1325	lung tissues	1314:1325	lung tissues	1314:1325	We found that treatments with SalB significantly inhibited increase in the tracheobronchial secretion, glycosaminoglycan levels, interleukin (IL)-13, IL-4, and IL-5 cytokines mRNA and protein expression, and decrease in mucociliary clearance in lung tissues.
29382534	8	28	from	decrease	1277:1284	arg1	secretion					1161:1169	the tracheobronchial secretion	1140:1169	the tracheobronchial secretion	1140:1169	We found that treatments with SalB significantly inhibited increase in the tracheobronchial secretion, glycosaminoglycan levels, interleukin (IL)-13, IL-4, and IL-5 cytokines mRNA and protein expression, and decrease in mucociliary clearance in lung tissues.
29382534	11	29	from	expression	1678:1687	arg1	cells					1709:1713	human epithelial cells	1692:1713	human epithelial cells	1692:1713	In vitro, SalB significantly inhibited IL-13-induced MUC5AC and MUC5B mRNA and protein expression in human epithelial cells.
29382534	2	30	theme	cardiovascular	314:327	arg1	lung					330:333	lung	330:333	lung	330:333	It is used for clinical treatment of various diseases including cardiovascular, lung, Liver, renal and cancers.
29382534	2	31	used	used	256:259	arg2	It					250:251	It	250:251	It	250:251	It is used for clinical treatment of various diseases including cardiovascular, lung, Liver, renal and cancers.
29382534	1	32	theme	water-soluble	174:186	arg1	composites					188:197	the main water-soluble composites	165:197	the main water-soluble composites from Chinese medicine Dansen (Radix miltiorrhiza)	165:247	Salvianolic acid B (SalB) is one of the main water-soluble composites from Chinese medicine Dansen (Radix miltiorrhiza).
29382534	0	33	theme	Salvianolic	0:10	arg1	B					17:17	Salvianolic acid B	0:17	Salvianolic acid B	0:17	Salvianolic acid B improves airway hyperresponsiveness by inhibiting MUC5AC overproduction associated with Erk1/2/P38 signaling.
29382534	10	34	theme	OVA-challenged	1567:1580	arg1	animals					1582:1588	OVA-challenged animals	1567:1588	OVA-challenged animals	1567:1588	SalB exhibited protective effects against AHR in OVA-challenged animals.
29382534	8	35	theme	interleukin	1198:1208	arg1	IL					1211:1212	interleukin (IL)-13	1198:1216	interleukin (IL)-13	1198:1216	We found that treatments with SalB significantly inhibited increase in the tracheobronchial secretion, glycosaminoglycan levels, interleukin (IL)-13, IL-4, and IL-5 cytokines mRNA and protein expression, and decrease in mucociliary clearance in lung tissues.
29382534	8	36	theme	mucociliary	1289:1299	arg1	clearance					1301:1309	mucociliary clearance	1289:1309	mucociliary clearance	1289:1309	We found that treatments with SalB significantly inhibited increase in the tracheobronchial secretion, glycosaminoglycan levels, interleukin (IL)-13, IL-4, and IL-5 cytokines mRNA and protein expression, and decrease in mucociliary clearance in lung tissues.
29382534	3	37	theme	induced	402:408	arg1	hypersecretion					423:436	allergy induced airway mucin hypersecretion	394:436	allergy induced airway mucin hypersecretion	394:436	However, the effects of SalB to allergy induced airway mucin hypersecretion, inflammation and hyperresponsiveness (AHR) remains not clear.
29382534	8	38	theme	tracheobronchial	1144:1159	arg1	secretion					1161:1169	the tracheobronchial secretion	1140:1169	the tracheobronchial secretion	1140:1169	We found that treatments with SalB significantly inhibited increase in the tracheobronchial secretion, glycosaminoglycan levels, interleukin (IL)-13, IL-4, and IL-5 cytokines mRNA and protein expression, and decrease in mucociliary clearance in lung tissues.
29382534	9	39	theme	OVA-induced	1383:1393	arg1	infiltration					1406:1417	OVA-induced eosinophil infiltration	1383:1417	OVA-induced eosinophil infiltration	1383:1417	Histological results demonstrated that SalB attenuated OVA-induced eosinophil infiltration, airway goblet cell hyperplasia, and MUC5AC and MUC5B mRNA and protein expression in lung tissues.
29382534	2	40	theme	diseases	295:302	arg1	treatment					274:282	clinical treatment	265:282	clinical treatment of various diseases including cardiovascular, lung, Liver, renal and cancers	265:359	It is used for clinical treatment of various diseases including cardiovascular, lung, Liver, renal and cancers.
29382534	4	41	theme	asthmatic	611:619	arg1	attack					621:626	asthmatic attack	611:626	asthmatic attack	611:626	Overproduction of airway MUC5AC is a central effector of inflammation that is strongly associated with AHR in asthmatic attack.
29382534	9	42	theme	lung	1504:1507	arg1	tissues					1509:1515	lung tissues	1504:1515	lung tissues	1504:1515	Histological results demonstrated that SalB attenuated OVA-induced eosinophil infiltration, airway goblet cell hyperplasia, and MUC5AC and MUC5B mRNA and protein expression in lung tissues.
29382534	1	43	theme	Salvianolic	129:139	arg1	SalB					149:152	SalB	149:152	SalB	149:152	Salvianolic acid B (SalB) is one of the main water-soluble composites from Chinese medicine Dansen (Radix miltiorrhiza).
29382534	1	43	theme	Salvianolic	129:139	arg1	B					146:146	Salvianolic acid B	129:146	Salvianolic acid B (SalB)	129:153	Salvianolic acid B (SalB) is one of the main water-soluble composites from Chinese medicine Dansen (Radix miltiorrhiza).
29382534	9	44	theme	protein	1482:1488	arg1	expression					1490:1499	mRNA and protein expression	1473:1499	expression	1490:1499	Histological results demonstrated that SalB attenuated OVA-induced eosinophil infiltration, airway goblet cell hyperplasia, and MUC5AC and MUC5B mRNA and protein expression in lung tissues.
29382534	1	45	theme	Chinese	204:210	arg1	medicine					212:219	Chinese medicine Dansen	204:226	Chinese medicine Dansen (Radix miltiorrhiza)	204:247	Salvianolic acid B (SalB) is one of the main water-soluble composites from Chinese medicine Dansen (Radix miltiorrhiza).
29382534	3	46	theme	SalB	386:389	arg1	effects					375:381	the effects	371:381	the effects of SalB to allergy induced airway mucin hypersecretion, inflammation and hyperresponsiveness (AHR)	371:480	However, the effects of SalB to allergy induced airway mucin hypersecretion, inflammation and hyperresponsiveness (AHR) remains not clear.
29382534	4	47	theme	central	538:544	arg1	Overproduction					501:514	Overproduction	501:514	Overproduction of airway MUC5AC	501:531	Overproduction of airway MUC5AC is a central effector of inflammation that is strongly associated with AHR in asthmatic attack.
29382534	4	47	theme	central	538:544	arg1	effector					546:553	a central effector	536:553	a central effector of inflammation that is strongly associated with AHR in asthmatic attack	536:626	Overproduction of airway MUC5AC is a central effector of inflammation that is strongly associated with AHR in asthmatic attack.
29382534	8	48	theme	IL-4	1219:1222	arg1	mRNA					1244:1247	interleukin (IL)-13, IL-4, and IL-5 cytokines mRNA	1198:1247	interleukin (IL)-13, IL-4, and IL-5 cytokines mRNA	1198:1247	We found that treatments with SalB significantly inhibited increase in the tracheobronchial secretion, glycosaminoglycan levels, interleukin (IL)-13, IL-4, and IL-5 cytokines mRNA and protein expression, and decrease in mucociliary clearance in lung tissues.
29382534	8	48	theme	IL-4	1219:1222	arg1	levels					1190:1195	glycosaminoglycan levels	1172:1195	glycosaminoglycan levels	1172:1195	We found that treatments with SalB significantly inhibited increase in the tracheobronchial secretion, glycosaminoglycan levels, interleukin (IL)-13, IL-4, and IL-5 cytokines mRNA and protein expression, and decrease in mucociliary clearance in lung tissues.
29382534	9	49	dep	infiltration	1406:1417	arg1	expression					1490:1499	mRNA and protein expression	1473:1499	expression	1490:1499	Histological results demonstrated that SalB attenuated OVA-induced eosinophil infiltration, airway goblet cell hyperplasia, and MUC5AC and MUC5B mRNA and protein expression in lung tissues.
29382534	1	50	theme	composites	188:197	arg1	composites					188:197	the main water-soluble composites	165:197	the main water-soluble composites from Chinese medicine Dansen (Radix miltiorrhiza)	165:247	Salvianolic acid B (SalB) is one of the main water-soluble composites from Chinese medicine Dansen (Radix miltiorrhiza).
29382534	1	50	theme	composites	188:197	arg1	one					158:160	one	158:160	one	158:160	Salvianolic acid B (SalB) is one of the main water-soluble composites from Chinese medicine Dansen (Radix miltiorrhiza).
29382534	5	51	theme	airway	812:817	arg1	inflammation					819:830	airway inflammation	812:830	airway inflammation	812:830	In this study, we investigated the anti-asthmatic activity and mechanism of SalB in a murine model and human epithelial cells by monitoring changes in mucin expression and secretion, airway inflammation, AHR, and signaling pathways.
29382534	5	51	theme	airway	812:817	arg1	changes					769:775	changes	769:775	changes in mucin expression and secretion	769:809	In this study, we investigated the anti-asthmatic activity and mechanism of SalB in a murine model and human epithelial cells by monitoring changes in mucin expression and secretion, airway inflammation, AHR, and signaling pathways.
29382534	0	52	theme	airway	28:33	arg1	hyperresponsiveness					35:53	airway hyperresponsiveness	28:53	airway hyperresponsiveness	28:53	Salvianolic acid B improves airway hyperresponsiveness by inhibiting MUC5AC overproduction associated with Erk1/2/P38 signaling.
29382534	7	53	dep	24h	1031:1033	arg1	challenge					1058:1066	the last antigen challenge	1041:1066	the last antigen challenge	1041:1066	All examinations were performed 24h after the last antigen challenge.
29382534	11	54	theme	MUC5B	1655:1659	arg1	mRNA					1661:1664	MUC5AC and MUC5B mRNA	1644:1664	MUC5AC and MUC5B mRNA	1644:1664	In vitro, SalB significantly inhibited IL-13-induced MUC5AC and MUC5B mRNA and protein expression in human epithelial cells.
29382534	1	55	from	medicine	212:219	arg1	composites					188:197	the main water-soluble composites	165:197	the main water-soluble composites from Chinese medicine Dansen (Radix miltiorrhiza)	165:247	Salvianolic acid B (SalB) is one of the main water-soluble composites from Chinese medicine Dansen (Radix miltiorrhiza).
29382534	6	56	theme	ovalbumin	982:990	arg1	sensitization					965:977	sensitization	965:977	sensitization of ovalbumin (OVA)	965:996	SalB was administered by intragastric administration (i.g) daily for a week, starting at 21 days after sensitization of ovalbumin (OVA).
29382534	10	57	from	effects	1544:1550	arg1	animals					1582:1588	OVA-challenged animals	1567:1588	OVA-challenged animals	1567:1588	SalB exhibited protective effects against AHR in OVA-challenged animals.
29382534	9	58	theme	eosinophil	1395:1404	arg1	infiltration					1406:1417	OVA-induced eosinophil infiltration	1383:1417	OVA-induced eosinophil infiltration	1383:1417	Histological results demonstrated that SalB attenuated OVA-induced eosinophil infiltration, airway goblet cell hyperplasia, and MUC5AC and MUC5B mRNA and protein expression in lung tissues.
29382534	11	59	theme	mRNA	1661:1664	arg1	expression					1678:1687	IL-13-induced MUC5AC and MUC5B mRNA and protein expression	1630:1687	IL-13-induced MUC5AC and MUC5B mRNA and protein expression in human epithelial cells	1630:1713	In vitro, SalB significantly inhibited IL-13-induced MUC5AC and MUC5B mRNA and protein expression in human epithelial cells.
29382534	9	60	theme	cell	1434:1437	arg1	hyperplasia					1439:1449	airway goblet cell hyperplasia	1420:1449	airway goblet cell hyperplasia	1420:1449	Histological results demonstrated that SalB attenuated OVA-induced eosinophil infiltration, airway goblet cell hyperplasia, and MUC5AC and MUC5B mRNA and protein expression in lung tissues.
29382534	1	61	theme	acid	141:144	arg1	SalB					149:152	SalB	149:152	SalB	149:152	Salvianolic acid B (SalB) is one of the main water-soluble composites from Chinese medicine Dansen (Radix miltiorrhiza).
29382534	1	61	theme	acid	141:144	arg1	B					146:146	Salvianolic acid B	129:146	Salvianolic acid B (SalB)	129:153	Salvianolic acid B (SalB) is one of the main water-soluble composites from Chinese medicine Dansen (Radix miltiorrhiza).
29382534	8	62	theme	glycosaminoglycan	1172:1188	arg1	expression					1261:1270	protein expression	1253:1270	protein expression	1253:1270	We found that treatments with SalB significantly inhibited increase in the tracheobronchial secretion, glycosaminoglycan levels, interleukin (IL)-13, IL-4, and IL-5 cytokines mRNA and protein expression, and decrease in mucociliary clearance in lung tissues.
29382534	8	62	theme	glycosaminoglycan	1172:1188	arg1	mRNA					1244:1247	interleukin (IL)-13, IL-4, and IL-5 cytokines mRNA	1198:1247	interleukin (IL)-13, IL-4, and IL-5 cytokines mRNA	1198:1247	We found that treatments with SalB significantly inhibited increase in the tracheobronchial secretion, glycosaminoglycan levels, interleukin (IL)-13, IL-4, and IL-5 cytokines mRNA and protein expression, and decrease in mucociliary clearance in lung tissues.
29382534	8	62	theme	glycosaminoglycan	1172:1188	arg1	levels					1190:1195	glycosaminoglycan levels	1172:1195	glycosaminoglycan levels	1172:1195	We found that treatments with SalB significantly inhibited increase in the tracheobronchial secretion, glycosaminoglycan levels, interleukin (IL)-13, IL-4, and IL-5 cytokines mRNA and protein expression, and decrease in mucociliary clearance in lung tissues.
29382534	4	63	theme	MUC5AC	526:531	arg1	Overproduction					501:514	Overproduction	501:514	Overproduction of airway MUC5AC	501:531	Overproduction of airway MUC5AC is a central effector of inflammation that is strongly associated with AHR in asthmatic attack.
29382534	4	63	theme	MUC5AC	526:531	arg1	effector					546:553	a central effector	536:553	a central effector of inflammation that is strongly associated with AHR in asthmatic attack	536:626	Overproduction of airway MUC5AC is a central effector of inflammation that is strongly associated with AHR in asthmatic attack.
29382534	11	64	theme	human	1692:1696	arg1	cells					1709:1713	human epithelial cells	1692:1713	human epithelial cells	1692:1713	In vitro, SalB significantly inhibited IL-13-induced MUC5AC and MUC5B mRNA and protein expression in human epithelial cells.
29382534	4	65	theme	airway	519:524	arg1	MUC5AC					526:531	airway MUC5AC	519:531	airway MUC5AC	519:531	Overproduction of airway MUC5AC is a central effector of inflammation that is strongly associated with AHR in asthmatic attack.
29382534	11	66	theme	protein	1670:1676	arg1	expression					1678:1687	IL-13-induced MUC5AC and MUC5B mRNA and protein expression	1630:1687	IL-13-induced MUC5AC and MUC5B mRNA and protein expression in human epithelial cells	1630:1713	In vitro, SalB significantly inhibited IL-13-induced MUC5AC and MUC5B mRNA and protein expression in human epithelial cells.
29382534	11	67	theme	epithelial	1698:1707	arg1	cells					1709:1713	human epithelial cells	1692:1713	human epithelial cells	1692:1713	In vitro, SalB significantly inhibited IL-13-induced MUC5AC and MUC5B mRNA and protein expression in human epithelial cells.
29382534	1	68	dep	medicine	212:219	arg1	miltiorrhiza					235:246	Radix miltiorrhiza	229:246	Radix miltiorrhiza	229:246	Salvianolic acid B (SalB) is one of the main water-soluble composites from Chinese medicine Dansen (Radix miltiorrhiza).
29382534	12	69	theme	signaling	1788:1796	arg1	pathways					1798:1805	the Erk1/2 and P38 signaling pathways	1769:1805	the Erk1/2 and P38 signaling pathways	1769:1805	These effects were blocked by SalB by downregulating the Erk1/2 and P38 signaling pathways.
29382534	8	70	theme	cytokines	1234:1242	arg1	mRNA					1244:1247	interleukin (IL)-13, IL-4, and IL-5 cytokines mRNA	1198:1247	interleukin (IL)-13, IL-4, and IL-5 cytokines mRNA	1198:1247	We found that treatments with SalB significantly inhibited increase in the tracheobronchial secretion, glycosaminoglycan levels, interleukin (IL)-13, IL-4, and IL-5 cytokines mRNA and protein expression, and decrease in mucociliary clearance in lung tissues.
29382534	8	70	theme	cytokines	1234:1242	arg1	levels					1190:1195	glycosaminoglycan levels	1172:1195	glycosaminoglycan levels	1172:1195	We found that treatments with SalB significantly inhibited increase in the tracheobronchial secretion, glycosaminoglycan levels, interleukin (IL)-13, IL-4, and IL-5 cytokines mRNA and protein expression, and decrease in mucociliary clearance in lung tissues.
29382534	8	71	theme	lung	1314:1317	arg1	tissues					1319:1325	lung tissues	1314:1325	lung tissues	1314:1325	We found that treatments with SalB significantly inhibited increase in the tracheobronchial secretion, glycosaminoglycan levels, interleukin (IL)-13, IL-4, and IL-5 cytokines mRNA and protein expression, and decrease in mucociliary clearance in lung tissues.
29382534	5	72	theme	anti-asthmatic	664:677	arg1	activity					679:686	the anti-asthmatic activity	660:686	the anti-asthmatic activity	660:686	In this study, we investigated the anti-asthmatic activity and mechanism of SalB in a murine model and human epithelial cells by monitoring changes in mucin expression and secretion, airway inflammation, AHR, and signaling pathways.
29382534	12	73	theme	P38	1784:1786	arg1	pathways					1798:1805	the Erk1/2 and P38 signaling pathways	1769:1805	the Erk1/2 and P38 signaling pathways	1769:1805	These effects were blocked by SalB by downregulating the Erk1/2 and P38 signaling pathways.
29382534	8	74	theme	IL-5	1229:1232	arg1	cytokines					1234:1242	IL-5 cytokines	1229:1242	IL-5 cytokines	1229:1242	We found that treatments with SalB significantly inhibited increase in the tracheobronchial secretion, glycosaminoglycan levels, interleukin (IL)-13, IL-4, and IL-5 cytokines mRNA and protein expression, and decrease in mucociliary clearance in lung tissues.
29382534	4	75	from	AHR	604:606	arg1	attack					621:626	asthmatic attack	611:626	asthmatic attack	611:626	Overproduction of airway MUC5AC is a central effector of inflammation that is strongly associated with AHR in asthmatic attack.
29382534	9	76	theme	airway	1420:1425	arg1	hyperplasia					1439:1449	airway goblet cell hyperplasia	1420:1449	airway goblet cell hyperplasia	1420:1449	Histological results demonstrated that SalB attenuated OVA-induced eosinophil infiltration, airway goblet cell hyperplasia, and MUC5AC and MUC5B mRNA and protein expression in lung tissues.
29382534	5	77	theme	signaling	842:850	arg1	changes					769:775	changes	769:775	changes in mucin expression and secretion	769:809	In this study, we investigated the anti-asthmatic activity and mechanism of SalB in a murine model and human epithelial cells by monitoring changes in mucin expression and secretion, airway inflammation, AHR, and signaling pathways.
29382534	5	77	theme	signaling	842:850	arg1	pathways					852:859	signaling pathways	842:859	signaling pathways	842:859	In this study, we investigated the anti-asthmatic activity and mechanism of SalB in a murine model and human epithelial cells by monitoring changes in mucin expression and secretion, airway inflammation, AHR, and signaling pathways.
29382534	3	78	theme	mucin	417:421	arg1	hypersecretion					423:436	allergy induced airway mucin hypersecretion	394:436	allergy induced airway mucin hypersecretion	394:436	However, the effects of SalB to allergy induced airway mucin hypersecretion, inflammation and hyperresponsiveness (AHR) remains not clear.
29382534	9	79	theme	Histological	1328:1339	arg1	results					1341:1347	Histological results	1328:1347	Histological results	1328:1347	Histological results demonstrated that SalB attenuated OVA-induced eosinophil infiltration, airway goblet cell hyperplasia, and MUC5AC and MUC5B mRNA and protein expression in lung tissues.
29382534	0	80	theme	MUC5AC	69:74	arg1	overproduction					76:89	MUC5AC overproduction	69:89	MUC5AC overproduction associated with Erk1/2/P38 signaling	69:126	Salvianolic acid B improves airway hyperresponsiveness by inhibiting MUC5AC overproduction associated with Erk1/2/P38 signaling.
29382534	6	81	theme	intragastric	887:898	arg1	i.g					916:918	i.g	916:918	i.g	916:918	SalB was administered by intragastric administration (i.g) daily for a week, starting at 21 days after sensitization of ovalbumin (OVA).
29382534	6	81	theme	intragastric	887:898	arg1	administration					900:913	intragastric administration	887:913	intragastric administration (i.g)	887:919	SalB was administered by intragastric administration (i.g) daily for a week, starting at 21 days after sensitization of ovalbumin (OVA).
29382534	12	82	theme	Erk1/2	1773:1778	arg1	pathways					1798:1805	the Erk1/2 and P38 signaling pathways	1769:1805	the Erk1/2 and P38 signaling pathways	1769:1805	These effects were blocked by SalB by downregulating the Erk1/2 and P38 signaling pathways.
29382534	9	83	theme	mRNA	1473:1476	arg1	expression					1490:1499	mRNA and protein expression	1473:1499	expression	1490:1499	Histological results demonstrated that SalB attenuated OVA-induced eosinophil infiltration, airway goblet cell hyperplasia, and MUC5AC and MUC5B mRNA and protein expression in lung tissues.
31617295	1	0	theme	guaranteeing	329:340	arg1	efficacy					347:354	guaranteeing drug efficacy	329:354	guaranteeing drug efficacy	329:354	Cervical cancer treatment is subject to limited drug access to locally diseased targets and generally resistant to chemotherapy, thus it is essential to develop a local drug delivery system to overcome these problems, premised on guaranteeing drug efficacy.
31617295	1	1	theme	drug	342:345	arg1	efficacy					347:354	guaranteeing drug efficacy	329:354	guaranteeing drug efficacy	329:354	Cervical cancer treatment is subject to limited drug access to locally diseased targets and generally resistant to chemotherapy, thus it is essential to develop a local drug delivery system to overcome these problems, premised on guaranteeing drug efficacy.
31617295	1	2	theme	diseased	170:177	arg1	targets					179:185	locally diseased targets	162:185	locally diseased targets	162:185	Cervical cancer treatment is subject to limited drug access to locally diseased targets and generally resistant to chemotherapy, thus it is essential to develop a local drug delivery system to overcome these problems, premised on guaranteeing drug efficacy.
31617295	5	3	theme	cancer	1051:1056	arg1	model					1058:1062	the orthotopic cervical cancer model	1027:1062	the orthotopic cervical cancer model	1027:1062	More to the point, as proved in the in vivo experiments, the retention time in the vagina is prolonged and the tumor growth is effectively suppressed by the nanogel without any side effects in the orthotopic cervical cancer model.
31617295	6	4	theme	cancer	1201:1206	arg1	treatment					1208:1216	cervical cancer treatment	1192:1216	cervical cancer treatment	1192:1216	As mentioned above, this novel mucoadhesive nanogel is believed to be a useful tool toward designing drug delivery systems for cervical cancer treatment.
31617295	5	5	theme	tumor	945:949	arg1	growth					951:956	the tumor growth	941:956	the tumor growth	941:956	More to the point, as proved in the in vivo experiments, the retention time in the vagina is prolonged and the tumor growth is effectively suppressed by the nanogel without any side effects in the orthotopic cervical cancer model.
31617295	1	6	theme	local	262:266	arg1	system					282:287	a local drug delivery system	260:287	a local drug delivery system to overcome these problems	260:314	Cervical cancer treatment is subject to limited drug access to locally diseased targets and generally resistant to chemotherapy, thus it is essential to develop a local drug delivery system to overcome these problems, premised on guaranteeing drug efficacy.
31617295	4	7	theme	multidrug	749:757	arg1	resistance					759:768	multidrug resistance	749:768	multidrug resistance	749:768	The in vitro experiments demonstrate that nanogel exerts high cytotoxicity to cancer cells, reverses multidrug resistance effectively, and successfully promotes the permeation of drugs.
31617295	1	8	theme	drug	268:271	arg1	system					282:287	a local drug delivery system	260:287	a local drug delivery system to overcome these problems	260:314	Cervical cancer treatment is subject to limited drug access to locally diseased targets and generally resistant to chemotherapy, thus it is essential to develop a local drug delivery system to overcome these problems, premised on guaranteeing drug efficacy.
31617295	4	9	theme	high	705:708	arg1	cytotoxicity					710:721	high cytotoxicity	705:721	high cytotoxicity	705:721	The in vitro experiments demonstrate that nanogel exerts high cytotoxicity to cancer cells, reverses multidrug resistance effectively, and successfully promotes the permeation of drugs.
31617295	3	10	theme	acrylic	528:534	arg1	poly					523:526	mucoadhesive poly	510:526	mucoadhesive poly(acrylic acid)	510:540	Briefly, the nanogel is constructed with mucoadhesive poly(acrylic acid) as the backbone and multiple inclusions between β-cyclodextrin and paclitaxel as the crosslinking points.
31617295	3	10	theme	acrylic	528:534	arg1	acid					536:539	acrylic acid	528:539	acrylic acid	528:539	Briefly, the nanogel is constructed with mucoadhesive poly(acrylic acid) as the backbone and multiple inclusions between β-cyclodextrin and paclitaxel as the crosslinking points.
31617295	5	11	theme	retention	895:903	arg1	prolonged					927:935	prolonged	927:935	prolonged	927:935	More to the point, as proved in the in vivo experiments, the retention time in the vagina is prolonged and the tumor growth is effectively suppressed by the nanogel without any side effects in the orthotopic cervical cancer model.
31617295	5	11	theme	retention	895:903	arg1	time					905:908	the retention time	891:908	the retention time in the vagina	891:922	More to the point, as proved in the in vivo experiments, the retention time in the vagina is prolonged and the tumor growth is effectively suppressed by the nanogel without any side effects in the orthotopic cervical cancer model.
31617295	6	12	theme	useful	1137:1142	arg1	tool					1144:1147	a useful tool	1135:1147	a useful tool toward designing drug delivery systems for cervical cancer treatment	1135:1216	As mentioned above, this novel mucoadhesive nanogel is believed to be a useful tool toward designing drug delivery systems for cervical cancer treatment.
31617295	6	12	theme	useful	1137:1142	arg1	nanogel					1109:1115	this novel mucoadhesive nanogel	1085:1115	this novel mucoadhesive nanogel	1085:1115	As mentioned above, this novel mucoadhesive nanogel is believed to be a useful tool toward designing drug delivery systems for cervical cancer treatment.
31617295	5	13	dep	in	870:871	arg1	vivo					873:876	vivo	873:876	vivo	873:876	More to the point, as proved in the in vivo experiments, the retention time in the vagina is prolonged and the tumor growth is effectively suppressed by the nanogel without any side effects in the orthotopic cervical cancer model.
31617295	0	14	theme	Paclitaxel-Based	2:17	arg1	Nanogel					32:38	A Paclitaxel-Based Mucoadhesive Nanogel	0:38	A Paclitaxel-Based Mucoadhesive Nanogel with Multivalent Interactions for Cervical Cancer Therapy	0:96	A Paclitaxel-Based Mucoadhesive Nanogel with Multivalent Interactions for Cervical Cancer Therapy.
31617295	1	15	theme	Cervical	99:106	arg1	cancer					108:113	Cervical cancer	99:113	Cervical cancer treatment	99:123	Cervical cancer treatment is subject to limited drug access to locally diseased targets and generally resistant to chemotherapy, thus it is essential to develop a local drug delivery system to overcome these problems, premised on guaranteeing drug efficacy.
31617295	5	16	theme	in	870:871	arg1	experiments					878:888	the in vivo experiments	866:888	the in vivo experiments	866:888	More to the point, as proved in the in vivo experiments, the retention time in the vagina is prolonged and the tumor growth is effectively suppressed by the nanogel without any side effects in the orthotopic cervical cancer model.
31617295	6	17	theme	delivery	1171:1178	arg1	systems					1180:1186	drug delivery systems	1166:1186	drug delivery systems for cervical cancer treatment	1166:1216	As mentioned above, this novel mucoadhesive nanogel is believed to be a useful tool toward designing drug delivery systems for cervical cancer treatment.
31617295	1	18	theme	delivery	273:280	arg1	system					282:287	a local drug delivery system	260:287	a local drug delivery system to overcome these problems	260:314	Cervical cancer treatment is subject to limited drug access to locally diseased targets and generally resistant to chemotherapy, thus it is essential to develop a local drug delivery system to overcome these problems, premised on guaranteeing drug efficacy.
31617295	2	19	from	goal	367:370	arg1	mind					375:378	mind	375:378	mind	375:378	With this goal in mind, a multivalent interactions-based mucoadhesive nanogel for vaginal delivery is proposed.
31617295	1	20	theme	cancer	108:113	arg1	treatment					115:123	Cervical cancer treatment	99:123	Cervical cancer treatment	99:123	Cervical cancer treatment is subject to limited drug access to locally diseased targets and generally resistant to chemotherapy, thus it is essential to develop a local drug delivery system to overcome these problems, premised on guaranteeing drug efficacy.
31617295	2	21	theme	vaginal	439:445	arg1	delivery					447:454	vaginal delivery	439:454	vaginal delivery	439:454	With this goal in mind, a multivalent interactions-based mucoadhesive nanogel for vaginal delivery is proposed.
31617295	5	22	from	time	905:908	arg1	vagina					917:922	the vagina	913:922	the vagina	913:922	More to the point, as proved in the in vivo experiments, the retention time in the vagina is prolonged and the tumor growth is effectively suppressed by the nanogel without any side effects in the orthotopic cervical cancer model.
31617295	5	23	from	effects	1016:1022	arg1	model					1058:1062	the orthotopic cervical cancer model	1027:1062	the orthotopic cervical cancer model	1027:1062	More to the point, as proved in the in vivo experiments, the retention time in the vagina is prolonged and the tumor growth is effectively suppressed by the nanogel without any side effects in the orthotopic cervical cancer model.
31617295	0	24	theme	Mucoadhesive	19:30	arg1	Nanogel					32:38	A Paclitaxel-Based Mucoadhesive Nanogel	0:38	A Paclitaxel-Based Mucoadhesive Nanogel with Multivalent Interactions for Cervical Cancer Therapy	0:96	A Paclitaxel-Based Mucoadhesive Nanogel with Multivalent Interactions for Cervical Cancer Therapy.
31617295	1	25	dep	essential	239:247	arg1	premised					317:324	premised	317:324	premised on guaranteeing drug efficacy	317:354	Cervical cancer treatment is subject to limited drug access to locally diseased targets and generally resistant to chemotherapy, thus it is essential to develop a local drug delivery system to overcome these problems, premised on guaranteeing drug efficacy.
31617295	3	26	theme	mucoadhesive	510:521	arg1	poly					523:526	mucoadhesive poly	510:526	mucoadhesive poly(acrylic acid)	510:540	Briefly, the nanogel is constructed with mucoadhesive poly(acrylic acid) as the backbone and multiple inclusions between β-cyclodextrin and paclitaxel as the crosslinking points.
31617295	3	26	theme	mucoadhesive	510:521	arg1	acid					536:539	acrylic acid	528:539	acrylic acid	528:539	Briefly, the nanogel is constructed with mucoadhesive poly(acrylic acid) as the backbone and multiple inclusions between β-cyclodextrin and paclitaxel as the crosslinking points.
31617295	2	27	theme	mucoadhesive	414:425	arg1	nanogel					427:433	a multivalent interactions-based mucoadhesive nanogel	381:433	a multivalent interactions-based mucoadhesive nanogel for vaginal delivery	381:454	With this goal in mind, a multivalent interactions-based mucoadhesive nanogel for vaginal delivery is proposed.
31617295	0	28	theme	Multivalent	45:55	arg1	Interactions					57:68	Multivalent Interactions	45:68	Multivalent Interactions	45:68	A Paclitaxel-Based Mucoadhesive Nanogel with Multivalent Interactions for Cervical Cancer Therapy.
31617295	4	29	theme	in	652:653	arg1	experiments					661:671	The in vitro experiments	648:671	The in vitro experiments	648:671	The in vitro experiments demonstrate that nanogel exerts high cytotoxicity to cancer cells, reverses multidrug resistance effectively, and successfully promotes the permeation of drugs.
31617295	4	30	dep	in	652:653	arg1	vitro					655:659	vitro	655:659	vitro	655:659	The in vitro experiments demonstrate that nanogel exerts high cytotoxicity to cancer cells, reverses multidrug resistance effectively, and successfully promotes the permeation of drugs.
31617295	2	31	theme	interactions-based	395:412	arg1	nanogel					427:433	a multivalent interactions-based mucoadhesive nanogel	381:433	a multivalent interactions-based mucoadhesive nanogel for vaginal delivery	381:454	With this goal in mind, a multivalent interactions-based mucoadhesive nanogel for vaginal delivery is proposed.
31617295	0	32	with	Nanogel	32:38	arg1	Interactions					57:68	Multivalent Interactions	45:68	Multivalent Interactions	45:68	A Paclitaxel-Based Mucoadhesive Nanogel with Multivalent Interactions for Cervical Cancer Therapy.
31617295	2	33	theme	multivalent	383:393	arg1	nanogel					427:433	a multivalent interactions-based mucoadhesive nanogel	381:433	a multivalent interactions-based mucoadhesive nanogel for vaginal delivery	381:454	With this goal in mind, a multivalent interactions-based mucoadhesive nanogel for vaginal delivery is proposed.
31617295	5	34	theme	side	1011:1014	arg1	effects					1016:1022	any side effects	1007:1022	any side effects in the orthotopic cervical cancer model	1007:1062	More to the point, as proved in the in vivo experiments, the retention time in the vagina is prolonged and the tumor growth is effectively suppressed by the nanogel without any side effects in the orthotopic cervical cancer model.
31617295	4	35	theme	cancer	726:731	arg1	cells					733:737	cancer cells	726:737	cancer cells	726:737	The in vitro experiments demonstrate that nanogel exerts high cytotoxicity to cancer cells, reverses multidrug resistance effectively, and successfully promotes the permeation of drugs.
31617295	6	36	theme	cervical	1192:1199	arg1	treatment					1208:1216	cervical cancer treatment	1192:1216	cervical cancer treatment	1192:1216	As mentioned above, this novel mucoadhesive nanogel is believed to be a useful tool toward designing drug delivery systems for cervical cancer treatment.
31617295	1	37	theme	limited	139:145	arg1	access					152:157	limited drug access	139:157	limited drug access to locally diseased targets	139:185	Cervical cancer treatment is subject to limited drug access to locally diseased targets and generally resistant to chemotherapy, thus it is essential to develop a local drug delivery system to overcome these problems, premised on guaranteeing drug efficacy.
31617295	6	38	theme	novel	1090:1094	arg1	tool					1144:1147	a useful tool	1135:1147	a useful tool toward designing drug delivery systems for cervical cancer treatment	1135:1216	As mentioned above, this novel mucoadhesive nanogel is believed to be a useful tool toward designing drug delivery systems for cervical cancer treatment.
31617295	6	38	theme	novel	1090:1094	arg1	nanogel					1109:1115	this novel mucoadhesive nanogel	1085:1115	this novel mucoadhesive nanogel	1085:1115	As mentioned above, this novel mucoadhesive nanogel is believed to be a useful tool toward designing drug delivery systems for cervical cancer treatment.
31617295	5	39	theme	orthotopic	1031:1040	arg1	model					1058:1062	the orthotopic cervical cancer model	1027:1062	the orthotopic cervical cancer model	1027:1062	More to the point, as proved in the in vivo experiments, the retention time in the vagina is prolonged and the tumor growth is effectively suppressed by the nanogel without any side effects in the orthotopic cervical cancer model.
31617295	3	40	theme	crosslinking	627:638	arg1	points					640:645	the crosslinking points	623:645	the crosslinking points	623:645	Briefly, the nanogel is constructed with mucoadhesive poly(acrylic acid) as the backbone and multiple inclusions between β-cyclodextrin and paclitaxel as the crosslinking points.
31617295	1	41	theme	drug	147:150	arg1	access					152:157	limited drug access	139:157	limited drug access to locally diseased targets	139:185	Cervical cancer treatment is subject to limited drug access to locally diseased targets and generally resistant to chemotherapy, thus it is essential to develop a local drug delivery system to overcome these problems, premised on guaranteeing drug efficacy.
31617295	6	42	theme	mucoadhesive	1096:1107	arg1	tool					1144:1147	a useful tool	1135:1147	a useful tool toward designing drug delivery systems for cervical cancer treatment	1135:1216	As mentioned above, this novel mucoadhesive nanogel is believed to be a useful tool toward designing drug delivery systems for cervical cancer treatment.
31617295	6	42	theme	mucoadhesive	1096:1107	arg1	nanogel					1109:1115	this novel mucoadhesive nanogel	1085:1115	this novel mucoadhesive nanogel	1085:1115	As mentioned above, this novel mucoadhesive nanogel is believed to be a useful tool toward designing drug delivery systems for cervical cancer treatment.
31617295	0	43	theme	Cancer	83:88	arg1	Therapy					90:96	Cervical Cancer Therapy	74:96	Cervical Cancer Therapy	74:96	A Paclitaxel-Based Mucoadhesive Nanogel with Multivalent Interactions for Cervical Cancer Therapy.
31617295	5	44	theme	cervical	1042:1049	arg1	model					1058:1062	the orthotopic cervical cancer model	1027:1062	the orthotopic cervical cancer model	1027:1062	More to the point, as proved in the in vivo experiments, the retention time in the vagina is prolonged and the tumor growth is effectively suppressed by the nanogel without any side effects in the orthotopic cervical cancer model.
31617295	4	45	theme	drugs	827:831	arg1	permeation					813:822	the permeation	809:822	the permeation of drugs	809:831	The in vitro experiments demonstrate that nanogel exerts high cytotoxicity to cancer cells, reverses multidrug resistance effectively, and successfully promotes the permeation of drugs.
31617295	0	46	theme	Cervical	74:81	arg1	Therapy					90:96	Cervical Cancer Therapy	74:96	Cervical Cancer Therapy	74:96	A Paclitaxel-Based Mucoadhesive Nanogel with Multivalent Interactions for Cervical Cancer Therapy.
31617295	6	47	theme	drug	1166:1169	arg1	systems					1180:1186	drug delivery systems	1166:1186	drug delivery systems for cervical cancer treatment	1166:1216	As mentioned above, this novel mucoadhesive nanogel is believed to be a useful tool toward designing drug delivery systems for cervical cancer treatment.
31617295	3	48	theme	multiple	562:569	arg1	inclusions					571:580	multiple inclusions	562:580	multiple inclusions between β-cyclodextrin and paclitaxel as the crosslinking points	562:645	Briefly, the nanogel is constructed with mucoadhesive poly(acrylic acid) as the backbone and multiple inclusions between β-cyclodextrin and paclitaxel as the crosslinking points.
31617295	3	48	theme	multiple	562:569	arg1	nanogel					482:488	the nanogel	478:488	the nanogel	478:488	Briefly, the nanogel is constructed with mucoadhesive poly(acrylic acid) as the backbone and multiple inclusions between β-cyclodextrin and paclitaxel as the crosslinking points.
30669427	4	0	dep	scaffolds	557:565	arg1	subjected					601:609	subjected	601:609	subjected to physicochemical and cell biological characterization	601:665	In this study, collagen fibril coatings enriched with HE800 and GY785 EPS derivatives were deposited on titanium alloy (Ti6Al4V) scaffolds produced by rapid prototyping and subjected to physicochemical and cell biological characterization.
30669427	4	0	dep	scaffolds	557:565	arg1	produced					567:574	produced	567:574	produced by rapid prototyping	567:595	In this study, collagen fibril coatings enriched with HE800 and GY785 EPS derivatives were deposited on titanium alloy (Ti6Al4V) scaffolds produced by rapid prototyping and subjected to physicochemical and cell biological characterization.
30669427	0	1	from	Coatings	31:38	arg1	Alloy					51:55	Alloy	51:55	Alloy	51:55	Marine Polysaccharide-Collagen Coatings on Ti6Al4V Alloy Formed by Self-Assembly.
30669427	9	2	theme	fibrils	1262:1268	arg1	growth					1197:1202	growth	1197:1202	growth	1197:1202	Cell biological characterization revealed good cell adhesion and growth on bare Ti6Al4V surfaces, as well as coatings of collagen fibrils only and collagen fibrils enhanced with HE800 and GY785 EPS derivatives.
30669427	9	2	theme	fibrils	1262:1268	arg1	adhesion					1184:1191	good cell adhesion	1174:1191	good cell adhesion	1174:1191	Cell biological characterization revealed good cell adhesion and growth on bare Ti6Al4V surfaces, as well as coatings of collagen fibrils only and collagen fibrils enhanced with HE800 and GY785 EPS derivatives.
30669427	3	3	theme	organic	344:350	arg1	coatings					375:382	organic, collagen fibril-based coatings	344:382	organic, collagen fibril-based coatings on biomaterial surfaces	344:406	The use of such polysaccharides as components of organic, collagen fibril-based coatings on biomaterial surfaces remains unexplored.
30669427	5	4	theme	self-assembly	694:706	arg1	process					708:714	a self-assembly process	692:714	a self-assembly process whereby polysaccharides were added to acidic collagen molecule solution, followed by neutralization to induced self-assembly of collagen fibrils	692:859	Coatings were formed by a self-assembly process whereby polysaccharides were added to acidic collagen molecule solution, followed by neutralization to induced self-assembly of collagen fibrils.
30669427	6	5	theme	collagen	891:898	arg1	formation					909:917	collagen hydrogel formation	891:917	collagen hydrogel formation	891:917	Fibril formation resulted in collagen hydrogel formation.
30669427	9	6	from	growth	1197:1202	arg1	coatings					1241:1248	coatings	1241:1248	coatings	1241:1248	Cell biological characterization revealed good cell adhesion and growth on bare Ti6Al4V surfaces, as well as coatings of collagen fibrils only and collagen fibrils enhanced with HE800 and GY785 EPS derivatives.
30669427	9	6	from	growth	1197:1202	arg1	surfaces					1220:1227	bare Ti6Al4V surfaces	1207:1227	bare Ti6Al4V surfaces	1207:1227	Cell biological characterization revealed good cell adhesion and growth on bare Ti6Al4V surfaces, as well as coatings of collagen fibrils only and collagen fibrils enhanced with HE800 and GY785 EPS derivatives.
30669427	5	7	dep	added	745:749	arg1	followed					789:796	followed	789:796	followed by neutralization to induced self-assembly of collagen fibrils	789:859	Coatings were formed by a self-assembly process whereby polysaccharides were added to acidic collagen molecule solution, followed by neutralization to induced self-assembly of collagen fibrils.
30669427	4	8	theme	alloy	541:545	arg1	scaffolds					557:565	titanium alloy (Ti6Al4V) scaffolds	532:565	titanium alloy (Ti6Al4V) scaffolds produced by rapid prototyping and subjected to physicochemical and cell biological characterization	532:665	In this study, collagen fibril coatings enriched with HE800 and GY785 EPS derivatives were deposited on titanium alloy (Ti6Al4V) scaffolds produced by rapid prototyping and subjected to physicochemical and cell biological characterization.
30669427	9	9	theme	collagen	1279:1286	arg1	fibrils					1288:1294	collagen fibrils	1279:1294	collagen fibrils	1279:1294	Cell biological characterization revealed good cell adhesion and growth on bare Ti6Al4V surfaces, as well as coatings of collagen fibrils only and collagen fibrils enhanced with HE800 and GY785 EPS derivatives.
30669427	9	10	theme	good	1174:1177	arg1	adhesion					1184:1191	good cell adhesion	1174:1191	good cell adhesion	1174:1191	Cell biological characterization revealed good cell adhesion and growth on bare Ti6Al4V surfaces, as well as coatings of collagen fibrils only and collagen fibrils enhanced with HE800 and GY785 EPS derivatives.
30669427	3	11	theme	biomaterial	387:397	arg1	surfaces					399:406	biomaterial surfaces	387:406	biomaterial surfaces	387:406	The use of such polysaccharides as components of organic, collagen fibril-based coatings on biomaterial surfaces remains unexplored.
30669427	3	12	from	surfaces	399:406	arg1	components					330:339	components	330:339	components of organic, collagen fibril-based coatings on biomaterial surfaces	330:406	The use of such polysaccharides as components of organic, collagen fibril-based coatings on biomaterial surfaces remains unexplored.
30669427	8	13	theme	coatings	1069:1076	arg1	analysis					1041:1048	Scanning electron microscopy (SEM) analysis	1006:1048	Scanning electron microscopy (SEM) analysis of collagen fibril coatings	1006:1076	Scanning electron microscopy (SEM) analysis of collagen fibril coatings revealed association of polysaccharides with fibrils.
30669427	10	14	theme	coating	1385:1391	arg1	components					1393:1402	coating components	1385:1402	coating components	1385:1402	Hence, the use of both EPS derivatives as coating components is feasible.
30669427	9	15	theme	cell	1179:1182	arg1	adhesion					1184:1191	good cell adhesion	1174:1191	good cell adhesion	1174:1191	Cell biological characterization revealed good cell adhesion and growth on bare Ti6Al4V surfaces, as well as coatings of collagen fibrils only and collagen fibrils enhanced with HE800 and GY785 EPS derivatives.
30669427	4	16	theme	physicochemical	614:628	arg1	characterization					650:665	physicochemical and cell biological characterization	614:665	physicochemical and cell biological characterization	614:665	In this study, collagen fibril coatings enriched with HE800 and GY785 EPS derivatives were deposited on titanium alloy (Ti6Al4V) scaffolds produced by rapid prototyping and subjected to physicochemical and cell biological characterization.
30669427	8	17	with	association	1087:1097	arg1	fibrils					1123:1129	fibrils	1123:1129	fibrils	1123:1129	Scanning electron microscopy (SEM) analysis of collagen fibril coatings revealed association of polysaccharides with fibrils.
30669427	4	18	theme	EPS	498:500	arg1	derivatives					502:512	GY785 EPS derivatives	492:512	GY785 EPS derivatives	492:512	In this study, collagen fibril coatings enriched with HE800 and GY785 EPS derivatives were deposited on titanium alloy (Ti6Al4V) scaffolds produced by rapid prototyping and subjected to physicochemical and cell biological characterization.
30669427	10	19	theme	derivatives	1370:1380	arg1	feasible					1407:1414	feasible	1407:1414	feasible	1407:1414	Hence, the use of both EPS derivatives as coating components is feasible.
30669427	10	19	theme	derivatives	1370:1380	arg1	use					1354:1356	the use	1350:1356	the use of both EPS derivatives as coating components	1350:1402	Hence, the use of both EPS derivatives as coating components is feasible.
30669427	8	20	theme	fibril	1062:1067	arg1	coatings					1069:1076	collagen fibril coatings	1053:1076	collagen fibril coatings	1053:1076	Scanning electron microscopy (SEM) analysis of collagen fibril coatings revealed association of polysaccharides with fibrils.
30669427	7	21	theme	Ti6Al4V	949:955	arg1	surfaces					957:964	Ti6Al4V surfaces	949:964	Ti6Al4V surfaces	949:964	Hydrogels formed directly on Ti6Al4V surfaces, and fibrils adsorbed onto the surface.
30669427	5	22	theme	collagen	761:768	arg1	solution					779:786	acidic collagen molecule solution	754:786	acidic collagen molecule solution	754:786	Coatings were formed by a self-assembly process whereby polysaccharides were added to acidic collagen molecule solution, followed by neutralization to induced self-assembly of collagen fibrils.
30669427	3	23	theme	coatings	375:382	arg1	components					330:339	components	330:339	components of organic, collagen fibril-based coatings on biomaterial surfaces	330:406	The use of such polysaccharides as components of organic, collagen fibril-based coatings on biomaterial surfaces remains unexplored.
30669427	6	24	theme	Fibril	862:867	arg1	formation					869:877	Fibril formation	862:877	Fibril formation	862:877	Fibril formation resulted in collagen hydrogel formation.
30669427	0	25	theme	Polysaccharide-Collagen	7:29	arg1	Coatings					31:38	Marine Polysaccharide-Collagen Coatings	0:38	Marine Polysaccharide-Collagen Coatings on Ti6Al4V Alloy	0:55	Marine Polysaccharide-Collagen Coatings on Ti6Al4V Alloy Formed by Self-Assembly.
30669427	1	26	theme	marine	101:106	arg1	origin					108:113	marine origin	101:113	marine origin	101:113	Polysaccharides of marine origin are gaining interest as biomaterial components.
30669427	0	27	theme	Marine	0:5	arg1	Coatings					31:38	Marine Polysaccharide-Collagen Coatings	0:38	Marine Polysaccharide-Collagen Coatings on Ti6Al4V Alloy	0:55	Marine Polysaccharide-Collagen Coatings on Ti6Al4V Alloy Formed by Self-Assembly.
30669427	4	28	theme	rapid	579:583	arg1	prototyping					585:595	rapid prototyping	579:595	rapid prototyping	579:595	In this study, collagen fibril coatings enriched with HE800 and GY785 EPS derivatives were deposited on titanium alloy (Ti6Al4V) scaffolds produced by rapid prototyping and subjected to physicochemical and cell biological characterization.
30669427	8	29	theme	polysaccharides	1102:1116	arg1	association					1087:1097	association	1087:1097	association of polysaccharides with fibrils	1087:1129	Scanning electron microscopy (SEM) analysis of collagen fibril coatings revealed association of polysaccharides with fibrils.
30669427	1	30	theme	origin	108:113	arg1	Polysaccharides					82:96	Polysaccharides	82:96	Polysaccharides of marine origin	82:113	Polysaccharides of marine origin are gaining interest as biomaterial components.
30669427	9	31	theme	EPS	1326:1328	arg1	derivatives					1330:1340	GY785 EPS derivatives	1320:1340	GY785 EPS derivatives	1320:1340	Cell biological characterization revealed good cell adhesion and growth on bare Ti6Al4V surfaces, as well as coatings of collagen fibrils only and collagen fibrils enhanced with HE800 and GY785 EPS derivatives.
30669427	5	32	theme	acidic	754:759	arg1	solution					779:786	acidic collagen molecule solution	754:786	acidic collagen molecule solution	754:786	Coatings were formed by a self-assembly process whereby polysaccharides were added to acidic collagen molecule solution, followed by neutralization to induced self-assembly of collagen fibrils.
30669427	9	33	theme	fibrils	1288:1294	arg1	growth					1197:1202	growth	1197:1202	growth	1197:1202	Cell biological characterization revealed good cell adhesion and growth on bare Ti6Al4V surfaces, as well as coatings of collagen fibrils only and collagen fibrils enhanced with HE800 and GY785 EPS derivatives.
30669427	9	33	theme	fibrils	1288:1294	arg1	adhesion					1184:1191	good cell adhesion	1174:1191	good cell adhesion	1174:1191	Cell biological characterization revealed good cell adhesion and growth on bare Ti6Al4V surfaces, as well as coatings of collagen fibrils only and collagen fibrils enhanced with HE800 and GY785 EPS derivatives.
30669427	8	34	theme	collagen	1053:1060	arg1	coatings					1069:1076	collagen fibril coatings	1053:1076	collagen fibril coatings	1053:1076	Scanning electron microscopy (SEM) analysis of collagen fibril coatings revealed association of polysaccharides with fibrils.
30669427	3	35	dep	organic	344:350	arg1	fibril-based					362:373	fibril-based	362:373	fibril-based	362:373	The use of such polysaccharides as components of organic, collagen fibril-based coatings on biomaterial surfaces remains unexplored.
30669427	9	36	theme	GY785	1320:1324	arg1	derivatives					1330:1340	GY785 EPS derivatives	1320:1340	GY785 EPS derivatives	1320:1340	Cell biological characterization revealed good cell adhesion and growth on bare Ti6Al4V surfaces, as well as coatings of collagen fibrils only and collagen fibrils enhanced with HE800 and GY785 EPS derivatives.
30669427	9	37	theme	Ti6Al4V	1212:1218	arg1	surfaces					1220:1227	bare Ti6Al4V surfaces	1207:1227	bare Ti6Al4V surfaces	1207:1227	Cell biological characterization revealed good cell adhesion and growth on bare Ti6Al4V surfaces, as well as coatings of collagen fibrils only and collagen fibrils enhanced with HE800 and GY785 EPS derivatives.
30669427	5	38	theme	collagen	844:851	arg1	fibrils					853:859	collagen fibrils	844:859	collagen fibrils	844:859	Coatings were formed by a self-assembly process whereby polysaccharides were added to acidic collagen molecule solution, followed by neutralization to induced self-assembly of collagen fibrils.
30669427	10	39	theme	EPS	1366:1368	arg1	derivatives					1370:1380	both EPS derivatives	1361:1380	both EPS derivatives	1361:1380	Hence, the use of both EPS derivatives as coating components is feasible.
30669427	5	40	theme	molecule	770:777	arg1	solution					779:786	acidic collagen molecule solution	754:786	acidic collagen molecule solution	754:786	Coatings were formed by a self-assembly process whereby polysaccharides were added to acidic collagen molecule solution, followed by neutralization to induced self-assembly of collagen fibrils.
30669427	3	41	from	coatings	375:382	arg1	surfaces					399:406	biomaterial surfaces	387:406	biomaterial surfaces	387:406	The use of such polysaccharides as components of organic, collagen fibril-based coatings on biomaterial surfaces remains unexplored.
30669427	5	42	theme	fibrils	853:859	arg1	self-assembly					827:839	induced self-assembly	819:839	induced self-assembly of collagen fibrils	819:859	Coatings were formed by a self-assembly process whereby polysaccharides were added to acidic collagen molecule solution, followed by neutralization to induced self-assembly of collagen fibrils.
30669427	11	43	theme	cell	1446:1449	arg1	differentiation					1451:1465	cell differentiation	1446:1465	cell differentiation	1446:1465	Further work should focus on cell differentiation.
30669427	2	44	theme	hydrothermal	194:205	arg1	vents					207:211	deep-sea hydrothermal vents	185:211	deep-sea hydrothermal vents	185:211	Bacteria derived from deep-sea hydrothermal vents can produce sulfated exopolysaccharides (EPS), which can influence cell behavior.
30669427	8	45	theme	Scanning	1006:1013	arg1	SEM					1036:1038	SEM	1036:1038	SEM	1036:1038	Scanning electron microscopy (SEM) analysis of collagen fibril coatings revealed association of polysaccharides with fibrils.
30669427	8	45	theme	Scanning	1006:1013	arg1	microscopy					1024:1033	Scanning electron microscopy	1006:1033	Scanning electron microscopy (SEM) analysis of collagen fibril coatings	1006:1076	Scanning electron microscopy (SEM) analysis of collagen fibril coatings revealed association of polysaccharides with fibrils.
30669427	2	46	theme	deep-sea	185:192	arg1	vents					207:211	deep-sea hydrothermal vents	185:211	deep-sea hydrothermal vents	185:211	Bacteria derived from deep-sea hydrothermal vents can produce sulfated exopolysaccharides (EPS), which can influence cell behavior.
30669427	2	47	attach	derived	172:178	arg1	vents					207:211	deep-sea hydrothermal vents	185:211	deep-sea hydrothermal vents	185:211	Bacteria derived from deep-sea hydrothermal vents can produce sulfated exopolysaccharides (EPS), which can influence cell behavior.
30669427	2	47	attach	derived	172:178	arg2	Bacteria					163:170	Bacteria	163:170	Bacteria derived from deep-sea hydrothermal vents	163:211	Bacteria derived from deep-sea hydrothermal vents can produce sulfated exopolysaccharides (EPS), which can influence cell behavior.
30669427	4	48	theme	fibril	452:457	arg1	coatings					459:466	collagen fibril coatings	443:466	collagen fibril coatings enriched with HE800 and GY785 EPS derivatives	443:512	In this study, collagen fibril coatings enriched with HE800 and GY785 EPS derivatives were deposited on titanium alloy (Ti6Al4V) scaffolds produced by rapid prototyping and subjected to physicochemical and cell biological characterization.
30669427	8	49	theme	microscopy	1024:1033	arg1	analysis					1041:1048	Scanning electron microscopy (SEM) analysis	1006:1048	Scanning electron microscopy (SEM) analysis of collagen fibril coatings	1006:1076	Scanning electron microscopy (SEM) analysis of collagen fibril coatings revealed association of polysaccharides with fibrils.
30669427	4	50	theme	GY785	492:496	arg1	derivatives					502:512	GY785 EPS derivatives	492:512	GY785 EPS derivatives	492:512	In this study, collagen fibril coatings enriched with HE800 and GY785 EPS derivatives were deposited on titanium alloy (Ti6Al4V) scaffolds produced by rapid prototyping and subjected to physicochemical and cell biological characterization.
30669427	3	51	theme	such	306:309	arg1	polysaccharides					311:325	such polysaccharides	306:325	such polysaccharides	306:325	The use of such polysaccharides as components of organic, collagen fibril-based coatings on biomaterial surfaces remains unexplored.
30669427	4	52	theme	collagen	443:450	arg1	coatings					459:466	collagen fibril coatings	443:466	collagen fibril coatings enriched with HE800 and GY785 EPS derivatives	443:512	In this study, collagen fibril coatings enriched with HE800 and GY785 EPS derivatives were deposited on titanium alloy (Ti6Al4V) scaffolds produced by rapid prototyping and subjected to physicochemical and cell biological characterization.
30669427	8	53	theme	electron	1015:1022	arg1	SEM					1036:1038	SEM	1036:1038	SEM	1036:1038	Scanning electron microscopy (SEM) analysis of collagen fibril coatings revealed association of polysaccharides with fibrils.
30669427	8	53	theme	electron	1015:1022	arg1	microscopy					1024:1033	Scanning electron microscopy	1006:1033	Scanning electron microscopy (SEM) analysis of collagen fibril coatings	1006:1076	Scanning electron microscopy (SEM) analysis of collagen fibril coatings revealed association of polysaccharides with fibrils.
30669427	1	54	theme	biomaterial	139:149	arg1	interest					127:134	interest	127:134	interest	127:134	Polysaccharides of marine origin are gaining interest as biomaterial components.
30669427	1	54	theme	biomaterial	139:149	arg1	components					151:160	biomaterial components	139:160	biomaterial components	139:160	Polysaccharides of marine origin are gaining interest as biomaterial components.
30669427	4	55	theme	biological	639:648	arg1	characterization					650:665	physicochemical and cell biological characterization	614:665	physicochemical and cell biological characterization	614:665	In this study, collagen fibril coatings enriched with HE800 and GY785 EPS derivatives were deposited on titanium alloy (Ti6Al4V) scaffolds produced by rapid prototyping and subjected to physicochemical and cell biological characterization.
30669427	3	56	theme	polysaccharides	311:325	arg1	use					299:301	The use	295:301	The use of such polysaccharides as components of organic, collagen fibril-based coatings on biomaterial surfaces	295:406	The use of such polysaccharides as components of organic, collagen fibril-based coatings on biomaterial surfaces remains unexplored.
30669427	2	57	theme	sulfated	225:232	arg1	EPS					254:256	EPS	254:256	EPS	254:256	Bacteria derived from deep-sea hydrothermal vents can produce sulfated exopolysaccharides (EPS), which can influence cell behavior.
30669427	2	57	theme	sulfated	225:232	arg1	exopolysaccharides					234:251	sulfated exopolysaccharides	225:251	sulfated exopolysaccharides (EPS)	225:257	Bacteria derived from deep-sea hydrothermal vents can produce sulfated exopolysaccharides (EPS), which can influence cell behavior.
30669427	2	58	theme	cell	280:283	arg1	behavior					285:292	cell behavior	280:292	cell behavior	280:292	Bacteria derived from deep-sea hydrothermal vents can produce sulfated exopolysaccharides (EPS), which can influence cell behavior.
30669427	9	59	theme	bare	1207:1210	arg1	surfaces					1220:1227	bare Ti6Al4V surfaces	1207:1227	bare Ti6Al4V surfaces	1207:1227	Cell biological characterization revealed good cell adhesion and growth on bare Ti6Al4V surfaces, as well as coatings of collagen fibrils only and collagen fibrils enhanced with HE800 and GY785 EPS derivatives.
30669427	6	60	theme	hydrogel	900:907	arg1	formation					909:917	collagen hydrogel formation	891:917	collagen hydrogel formation	891:917	Fibril formation resulted in collagen hydrogel formation.
30669427	9	61	theme	Cell	1132:1135	arg1	characterization					1148:1163	Cell biological characterization	1132:1163	Cell biological characterization	1132:1163	Cell biological characterization revealed good cell adhesion and growth on bare Ti6Al4V surfaces, as well as coatings of collagen fibrils only and collagen fibrils enhanced with HE800 and GY785 EPS derivatives.
30669427	5	62	theme	induced	819:825	arg1	self-assembly					827:839	induced self-assembly	819:839	induced self-assembly of collagen fibrils	819:859	Coatings were formed by a self-assembly process whereby polysaccharides were added to acidic collagen molecule solution, followed by neutralization to induced self-assembly of collagen fibrils.
30669427	4	63	theme	titanium	532:539	arg1	Ti6Al4V					548:554	Ti6Al4V	548:554	Ti6Al4V	548:554	In this study, collagen fibril coatings enriched with HE800 and GY785 EPS derivatives were deposited on titanium alloy (Ti6Al4V) scaffolds produced by rapid prototyping and subjected to physicochemical and cell biological characterization.
30669427	4	63	theme	titanium	532:539	arg1	alloy					541:545	titanium alloy	532:545	titanium alloy (Ti6Al4V) scaffolds produced by rapid prototyping and subjected to physicochemical and cell biological characterization	532:665	In this study, collagen fibril coatings enriched with HE800 and GY785 EPS derivatives were deposited on titanium alloy (Ti6Al4V) scaffolds produced by rapid prototyping and subjected to physicochemical and cell biological characterization.
30669427	9	64	from	adhesion	1184:1191	arg1	coatings					1241:1248	coatings	1241:1248	coatings	1241:1248	Cell biological characterization revealed good cell adhesion and growth on bare Ti6Al4V surfaces, as well as coatings of collagen fibrils only and collagen fibrils enhanced with HE800 and GY785 EPS derivatives.
30669427	9	64	from	adhesion	1184:1191	arg1	surfaces					1220:1227	bare Ti6Al4V surfaces	1207:1227	bare Ti6Al4V surfaces	1207:1227	Cell biological characterization revealed good cell adhesion and growth on bare Ti6Al4V surfaces, as well as coatings of collagen fibrils only and collagen fibrils enhanced with HE800 and GY785 EPS derivatives.
30669427	9	65	theme	collagen	1253:1260	arg1	fibrils					1262:1268	collagen fibrils only	1253:1273	collagen fibrils only	1253:1273	Cell biological characterization revealed good cell adhesion and growth on bare Ti6Al4V surfaces, as well as coatings of collagen fibrils only and collagen fibrils enhanced with HE800 and GY785 EPS derivatives.
30669427	11	66	theme	Further	1417:1423	arg1	work					1425:1428	Further work	1417:1428	Further work	1417:1428	Further work should focus on cell differentiation.
30669427	9	67	theme	biological	1137:1146	arg1	characterization					1148:1163	Cell biological characterization	1132:1163	Cell biological characterization	1132:1163	Cell biological characterization revealed good cell adhesion and growth on bare Ti6Al4V surfaces, as well as coatings of collagen fibrils only and collagen fibrils enhanced with HE800 and GY785 EPS derivatives.
30669427	3	68	from	components	330:339	arg1	surfaces					399:406	biomaterial surfaces	387:406	biomaterial surfaces	387:406	The use of such polysaccharides as components of organic, collagen fibril-based coatings on biomaterial surfaces remains unexplored.
31646641	5	0	theme	compounds	689:697	arg1	Release					646:652	Release	646:652	Release of volatile compounds and phenolic compounds during the alcoholic fermentation	646:731	Release of volatile compounds and phenolic compounds during the alcoholic fermentation was monitored, and the finished wines were chemically characterized.
31646641	4	1	theme	red	614:616	arg1	winemaking					618:627	red winemaking	614:627	red winemaking	614:627	Grapes from both origins were characterized and used for red winemaking by the same way.
31646641	7	2	theme	terpenes	1041:1048	arg1	terpenes					1041:1048	terpenes	1041:1048	terpenes	1041:1048	Wines made from grapes with a greater proportion of skins contain higher amounts of phenolic compounds, terpenes, volatile acids, acetate esters and polysaccharides.
31646641	7	2	theme	terpenes	1041:1048	arg1	esters					1075:1080	acetate esters	1067:1080	acetate esters	1067:1080	Wines made from grapes with a greater proportion of skins contain higher amounts of phenolic compounds, terpenes, volatile acids, acetate esters and polysaccharides.
31646641	7	2	theme	terpenes	1041:1048	arg1	polysaccharides					1086:1100	polysaccharides	1086:1100	polysaccharides	1086:1100	Wines made from grapes with a greater proportion of skins contain higher amounts of phenolic compounds, terpenes, volatile acids, acetate esters and polysaccharides.
31646641	7	2	theme	terpenes	1041:1048	arg1	amounts					1010:1016	higher amounts	1003:1016	higher amounts of phenolic compounds, terpenes, volatile acids, acetate esters and polysaccharides	1003:1100	Wines made from grapes with a greater proportion of skins contain higher amounts of phenolic compounds, terpenes, volatile acids, acetate esters and polysaccharides.
31646641	7	2	theme	terpenes	1041:1048	arg1	acids					1060:1064	volatile acids	1051:1064	volatile acids	1051:1064	Wines made from grapes with a greater proportion of skins contain higher amounts of phenolic compounds, terpenes, volatile acids, acetate esters and polysaccharides.
31646641	7	2	theme	terpenes	1041:1048	arg1	compounds					1030:1038	phenolic compounds	1021:1038	phenolic compounds	1021:1038	Wines made from grapes with a greater proportion of skins contain higher amounts of phenolic compounds, terpenes, volatile acids, acetate esters and polysaccharides.
31646641	8	3	theme	berry	1219:1223	arg1	size					1225:1228	berry size	1219:1228	berry size	1219:1228	CONCLUSION According to the results, it seems that grape skin extraction is more related to skin proportion than to berry size.
31646641	6	4	theme	surface-to-volume	849:865	arg1	ratio					867:871	a lower theoretical surface-to-volume ratio	829:871	a lower theoretical surface-to-volume ratio	829:871	RESULTS Larger grapes have a lower theoretical surface-to-volume ratio but have thicker skins and a greater proportion of skins (m/m).
31646641	7	5	theme	volatile	1051:1058	arg1	acids					1060:1064	volatile acids	1051:1064	volatile acids	1051:1064	Wines made from grapes with a greater proportion of skins contain higher amounts of phenolic compounds, terpenes, volatile acids, acetate esters and polysaccharides.
31646641	2	6	from	harvest	278:284	arg1	tool					291:294	a tool	289:294	a tool to measure the grape's potential to obtain great red wines	289:353	The popularity of this postulate among winemakers leads them to consider berry size at harvest as a tool to measure the grape's potential to obtain great red wines.
31646641	2	6	from	harvest	278:284	arg1	size					270:273	berry size	264:273	berry size at harvest	264:284	The popularity of this postulate among winemakers leads them to consider berry size at harvest as a tool to measure the grape's potential to obtain great red wines.
31646641	7	7	theme	esters	1075:1080	arg1	terpenes					1041:1048	terpenes	1041:1048	terpenes	1041:1048	Wines made from grapes with a greater proportion of skins contain higher amounts of phenolic compounds, terpenes, volatile acids, acetate esters and polysaccharides.
31646641	7	7	theme	esters	1075:1080	arg1	esters					1075:1080	acetate esters	1067:1080	acetate esters	1067:1080	Wines made from grapes with a greater proportion of skins contain higher amounts of phenolic compounds, terpenes, volatile acids, acetate esters and polysaccharides.
31646641	7	7	theme	esters	1075:1080	arg1	polysaccharides					1086:1100	polysaccharides	1086:1100	polysaccharides	1086:1100	Wines made from grapes with a greater proportion of skins contain higher amounts of phenolic compounds, terpenes, volatile acids, acetate esters and polysaccharides.
31646641	7	7	theme	esters	1075:1080	arg1	amounts					1010:1016	higher amounts	1003:1016	higher amounts of phenolic compounds, terpenes, volatile acids, acetate esters and polysaccharides	1003:1100	Wines made from grapes with a greater proportion of skins contain higher amounts of phenolic compounds, terpenes, volatile acids, acetate esters and polysaccharides.
31646641	7	7	theme	esters	1075:1080	arg1	acids					1060:1064	volatile acids	1051:1064	volatile acids	1051:1064	Wines made from grapes with a greater proportion of skins contain higher amounts of phenolic compounds, terpenes, volatile acids, acetate esters and polysaccharides.
31646641	7	7	theme	esters	1075:1080	arg1	compounds					1030:1038	phenolic compounds	1021:1038	phenolic compounds	1021:1038	Wines made from grapes with a greater proportion of skins contain higher amounts of phenolic compounds, terpenes, volatile acids, acetate esters and polysaccharides.
31646641	0	8	from	Impact	0:5	arg1	composition					44:54	red wine composition	35:54	red wine composition	35:54	Impact of berry size at harvest on red wine composition: a winemaker's approach.
31646641	7	9	theme	compounds	1030:1038	arg1	terpenes					1041:1048	terpenes	1041:1048	terpenes	1041:1048	Wines made from grapes with a greater proportion of skins contain higher amounts of phenolic compounds, terpenes, volatile acids, acetate esters and polysaccharides.
31646641	7	9	theme	compounds	1030:1038	arg1	esters					1075:1080	acetate esters	1067:1080	acetate esters	1067:1080	Wines made from grapes with a greater proportion of skins contain higher amounts of phenolic compounds, terpenes, volatile acids, acetate esters and polysaccharides.
31646641	7	9	theme	compounds	1030:1038	arg1	polysaccharides					1086:1100	polysaccharides	1086:1100	polysaccharides	1086:1100	Wines made from grapes with a greater proportion of skins contain higher amounts of phenolic compounds, terpenes, volatile acids, acetate esters and polysaccharides.
31646641	7	9	theme	compounds	1030:1038	arg1	amounts					1010:1016	higher amounts	1003:1016	higher amounts of phenolic compounds, terpenes, volatile acids, acetate esters and polysaccharides	1003:1100	Wines made from grapes with a greater proportion of skins contain higher amounts of phenolic compounds, terpenes, volatile acids, acetate esters and polysaccharides.
31646641	7	9	theme	compounds	1030:1038	arg1	acids					1060:1064	volatile acids	1051:1064	volatile acids	1051:1064	Wines made from grapes with a greater proportion of skins contain higher amounts of phenolic compounds, terpenes, volatile acids, acetate esters and polysaccharides.
31646641	7	9	theme	compounds	1030:1038	arg1	compounds					1030:1038	phenolic compounds	1021:1038	phenolic compounds	1021:1038	Wines made from grapes with a greater proportion of skins contain higher amounts of phenolic compounds, terpenes, volatile acids, acetate esters and polysaccharides.
31646641	1	10	dep	classical	94:102	arg1	postulate					104:112	postulate	104:112	postulate	104:112	BACKGROUND A classical postulate of viticulture declares that small grape berries produce the best red wines.
31646641	6	11	theme	lower	831:835	arg1	ratio					867:871	a lower theoretical surface-to-volume ratio	829:871	a lower theoretical surface-to-volume ratio	829:871	RESULTS Larger grapes have a lower theoretical surface-to-volume ratio but have thicker skins and a greater proportion of skins (m/m).
31646641	0	12	dep	approach	71:78	arg1	Impact					0:5	Impact	0:5	Impact of berry size at harvest on red wine composition	0:54	Impact of berry size at harvest on red wine composition: a winemaker's approach.
31646641	6	13	theme	skins	924:928	arg1	skins					890:894	thicker skins	882:894	thicker skins	882:894	RESULTS Larger grapes have a lower theoretical surface-to-volume ratio but have thicker skins and a greater proportion of skins (m/m).
31646641	6	13	theme	skins	924:928	arg1	proportion					910:919	a greater proportion	900:919	a greater proportion of skins (m/m)	900:934	RESULTS Larger grapes have a lower theoretical surface-to-volume ratio but have thicker skins and a greater proportion of skins (m/m).
31646641	5	14	theme	volatile	657:664	arg1	compounds					666:674	volatile compounds	657:674	volatile compounds	657:674	Release of volatile compounds and phenolic compounds during the alcoholic fermentation was monitored, and the finished wines were chemically characterized.
31646641	6	15	contain	have	824:827	arg2	ratio					867:871	a lower theoretical surface-to-volume ratio	829:871	a lower theoretical surface-to-volume ratio	829:871	RESULTS Larger grapes have a lower theoretical surface-to-volume ratio but have thicker skins and a greater proportion of skins (m/m).
31646641	6	15	contain	have	824:827	arg1	RESULTS					802:808	RESULTS	802:808	RESULTS Larger grapes	802:822	RESULTS Larger grapes have a lower theoretical surface-to-volume ratio but have thicker skins and a greater proportion of skins (m/m).
31646641	1	16	dep	BACKGROUND	81:90	arg1	declares					129:136	declares	129:136	declares that small grape berries produce the best red wines	129:188	BACKGROUND A classical postulate of viticulture declares that small grape berries produce the best red wines.
31646641	1	17	theme	small	143:147	arg1	berries					155:161	small grape berries	143:161	small grape berries	143:161	BACKGROUND A classical postulate of viticulture declares that small grape berries produce the best red wines.
31646641	5	18	theme	compounds	666:674	arg1	Release					646:652	Release	646:652	Release of volatile compounds and phenolic compounds during the alcoholic fermentation	646:731	Release of volatile compounds and phenolic compounds during the alcoholic fermentation was monitored, and the finished wines were chemically characterized.
31646641	0	19	from	harvest	24:30	arg1	Impact					0:5	Impact	0:5	Impact of berry size at harvest on red wine composition	0:54	Impact of berry size at harvest on red wine composition: a winemaker's approach.
31646641	7	20	theme	phenolic	1021:1028	arg1	compounds					1030:1038	phenolic compounds	1021:1038	phenolic compounds	1021:1038	Wines made from grapes with a greater proportion of skins contain higher amounts of phenolic compounds, terpenes, volatile acids, acetate esters and polysaccharides.
31646641	3	21	from	harvest	548:554	arg1	size					540:543	average grape berry size	520:543	average grape berry size at harvest	520:554	To address this issue, two vineyards from the same cultivar and subjected to the same physiological conditions during growing were selected for their difference in average grape berry size at harvest.
31646641	3	21	from	harvest	548:554	arg1	difference					506:515	their difference	500:515	their difference in average grape berry size at harvest	500:554	To address this issue, two vineyards from the same cultivar and subjected to the same physiological conditions during growing were selected for their difference in average grape berry size at harvest.
31646641	1	22	theme	grape	149:153	arg1	berries					155:161	small grape berries	143:161	small grape berries	143:161	BACKGROUND A classical postulate of viticulture declares that small grape berries produce the best red wines.
31646641	7	23	theme	polysaccharides	1086:1100	arg1	terpenes					1041:1048	terpenes	1041:1048	terpenes	1041:1048	Wines made from grapes with a greater proportion of skins contain higher amounts of phenolic compounds, terpenes, volatile acids, acetate esters and polysaccharides.
31646641	7	23	theme	polysaccharides	1086:1100	arg1	esters					1075:1080	acetate esters	1067:1080	acetate esters	1067:1080	Wines made from grapes with a greater proportion of skins contain higher amounts of phenolic compounds, terpenes, volatile acids, acetate esters and polysaccharides.
31646641	7	23	theme	polysaccharides	1086:1100	arg1	polysaccharides					1086:1100	polysaccharides	1086:1100	polysaccharides	1086:1100	Wines made from grapes with a greater proportion of skins contain higher amounts of phenolic compounds, terpenes, volatile acids, acetate esters and polysaccharides.
31646641	7	23	theme	polysaccharides	1086:1100	arg1	amounts					1010:1016	higher amounts	1003:1016	higher amounts of phenolic compounds, terpenes, volatile acids, acetate esters and polysaccharides	1003:1100	Wines made from grapes with a greater proportion of skins contain higher amounts of phenolic compounds, terpenes, volatile acids, acetate esters and polysaccharides.
31646641	7	23	theme	polysaccharides	1086:1100	arg1	acids					1060:1064	volatile acids	1051:1064	volatile acids	1051:1064	Wines made from grapes with a greater proportion of skins contain higher amounts of phenolic compounds, terpenes, volatile acids, acetate esters and polysaccharides.
31646641	7	23	theme	polysaccharides	1086:1100	arg1	compounds					1030:1038	phenolic compounds	1021:1038	phenolic compounds	1021:1038	Wines made from grapes with a greater proportion of skins contain higher amounts of phenolic compounds, terpenes, volatile acids, acetate esters and polysaccharides.
31646641	7	24	theme	higher	1003:1008	arg1	terpenes					1041:1048	terpenes	1041:1048	terpenes	1041:1048	Wines made from grapes with a greater proportion of skins contain higher amounts of phenolic compounds, terpenes, volatile acids, acetate esters and polysaccharides.
31646641	7	24	theme	higher	1003:1008	arg1	esters					1075:1080	acetate esters	1067:1080	acetate esters	1067:1080	Wines made from grapes with a greater proportion of skins contain higher amounts of phenolic compounds, terpenes, volatile acids, acetate esters and polysaccharides.
31646641	7	24	theme	higher	1003:1008	arg1	polysaccharides					1086:1100	polysaccharides	1086:1100	polysaccharides	1086:1100	Wines made from grapes with a greater proportion of skins contain higher amounts of phenolic compounds, terpenes, volatile acids, acetate esters and polysaccharides.
31646641	7	24	theme	higher	1003:1008	arg1	amounts					1010:1016	higher amounts	1003:1016	higher amounts of phenolic compounds, terpenes, volatile acids, acetate esters and polysaccharides	1003:1100	Wines made from grapes with a greater proportion of skins contain higher amounts of phenolic compounds, terpenes, volatile acids, acetate esters and polysaccharides.
31646641	7	24	theme	higher	1003:1008	arg1	acids					1060:1064	volatile acids	1051:1064	volatile acids	1051:1064	Wines made from grapes with a greater proportion of skins contain higher amounts of phenolic compounds, terpenes, volatile acids, acetate esters and polysaccharides.
31646641	7	24	theme	higher	1003:1008	arg1	compounds					1030:1038	phenolic compounds	1021:1038	phenolic compounds	1021:1038	Wines made from grapes with a greater proportion of skins contain higher amounts of phenolic compounds, terpenes, volatile acids, acetate esters and polysaccharides.
31646641	3	25	from	cultivar	407:414	arg1	vineyards					383:391	vineyards	383:391	vineyards	383:391	To address this issue, two vineyards from the same cultivar and subjected to the same physiological conditions during growing were selected for their difference in average grape berry size at harvest.
31646641	7	26	theme	acetate	1067:1073	arg1	esters					1075:1080	acetate esters	1067:1080	acetate esters	1067:1080	Wines made from grapes with a greater proportion of skins contain higher amounts of phenolic compounds, terpenes, volatile acids, acetate esters and polysaccharides.
31646641	8	27	theme	grape	1154:1158	arg1	extraction					1165:1174	grape skin extraction	1154:1174	grape skin extraction	1154:1174	CONCLUSION According to the results, it seems that grape skin extraction is more related to skin proportion than to berry size.
31646641	9	28	theme	red	1277:1279	arg1	wines					1281:1285	darker red wines	1270:1285	darker red wines	1270:1285	Thus not always smaller grapes produce darker red wines.
31646641	4	29	from	origins	574:580	arg1	Grapes					557:562	Grapes	557:562	Grapes from both origins	557:580	Grapes from both origins were characterized and used for red winemaking by the same way.
31646641	8	30	theme	skin	1160:1163	arg1	extraction					1165:1174	grape skin extraction	1154:1174	grape skin extraction	1154:1174	CONCLUSION According to the results, it seems that grape skin extraction is more related to skin proportion than to berry size.
31646641	0	31	theme	size	16:19	arg1	Impact					0:5	Impact	0:5	Impact of berry size at harvest on red wine composition	0:54	Impact of berry size at harvest on red wine composition: a winemaker's approach.
31646641	10	32	theme	Chemical	1306:1313	arg1	Industry					1315:1322	Chemical Industry	1306:1322	Chemical Industry	1306:1322	© 2019 Society of Chemical Industry.
31646641	7	33	theme	skins	989:993	arg1	proportion					975:984	a greater proportion	965:984	a greater proportion of skins	965:993	Wines made from grapes with a greater proportion of skins contain higher amounts of phenolic compounds, terpenes, volatile acids, acetate esters and polysaccharides.
31646641	5	34	theme	finished	756:763	arg1	wines					765:769	the finished wines	752:769	the finished wines	752:769	Release of volatile compounds and phenolic compounds during the alcoholic fermentation was monitored, and the finished wines were chemically characterized.
31646641	0	35	theme	berry	10:14	arg1	size					16:19	berry size	10:19	berry size	10:19	Impact of berry size at harvest on red wine composition: a winemaker's approach.
31646641	6	36	theme	greater	902:908	arg1	proportion					910:919	a greater proportion	900:919	a greater proportion of skins (m/m)	900:934	RESULTS Larger grapes have a lower theoretical surface-to-volume ratio but have thicker skins and a greater proportion of skins (m/m).
31646641	6	37	theme	Larger	810:815	arg1	grapes					817:822	Larger grapes	810:822	RESULTS Larger grapes	802:822	RESULTS Larger grapes have a lower theoretical surface-to-volume ratio but have thicker skins and a greater proportion of skins (m/m).
31646641	2	38	theme	berry	264:268	arg1	tool					291:294	a tool	289:294	a tool to measure the grape's potential to obtain great red wines	289:353	The popularity of this postulate among winemakers leads them to consider berry size at harvest as a tool to measure the grape's potential to obtain great red wines.
31646641	2	38	theme	berry	264:268	arg1	size					270:273	berry size	264:273	berry size at harvest	264:284	The popularity of this postulate among winemakers leads them to consider berry size at harvest as a tool to measure the grape's potential to obtain great red wines.
31646641	7	39	contain	contain	995:1001	arg2	acids					1060:1064	volatile acids	1051:1064	volatile acids	1051:1064	Wines made from grapes with a greater proportion of skins contain higher amounts of phenolic compounds, terpenes, volatile acids, acetate esters and polysaccharides.
31646641	7	39	contain	contain	995:1001	arg2	compounds					1030:1038	phenolic compounds	1021:1038	phenolic compounds	1021:1038	Wines made from grapes with a greater proportion of skins contain higher amounts of phenolic compounds, terpenes, volatile acids, acetate esters and polysaccharides.
31646641	7	39	contain	contain	995:1001	arg1	Wines					937:941	Wines	937:941	Wines made from grapes with a greater proportion of skins	937:993	Wines made from grapes with a greater proportion of skins contain higher amounts of phenolic compounds, terpenes, volatile acids, acetate esters and polysaccharides.
31646641	7	39	contain	contain	995:1001	arg2	terpenes					1041:1048	terpenes	1041:1048	terpenes	1041:1048	Wines made from grapes with a greater proportion of skins contain higher amounts of phenolic compounds, terpenes, volatile acids, acetate esters and polysaccharides.
31646641	7	39	contain	contain	995:1001	arg2	esters					1075:1080	acetate esters	1067:1080	acetate esters	1067:1080	Wines made from grapes with a greater proportion of skins contain higher amounts of phenolic compounds, terpenes, volatile acids, acetate esters and polysaccharides.
31646641	7	39	contain	contain	995:1001	arg2	polysaccharides					1086:1100	polysaccharides	1086:1100	polysaccharides	1086:1100	Wines made from grapes with a greater proportion of skins contain higher amounts of phenolic compounds, terpenes, volatile acids, acetate esters and polysaccharides.
31646641	7	39	contain	contain	995:1001	arg2	amounts					1010:1016	higher amounts	1003:1016	higher amounts of phenolic compounds, terpenes, volatile acids, acetate esters and polysaccharides	1003:1100	Wines made from grapes with a greater proportion of skins contain higher amounts of phenolic compounds, terpenes, volatile acids, acetate esters and polysaccharides.
31646641	1	40	theme	best	175:178	arg1	wines					184:188	the best red wines	171:188	the best red wines	171:188	BACKGROUND A classical postulate of viticulture declares that small grape berries produce the best red wines.
31646641	3	41	theme	grape	528:532	arg1	size					540:543	average grape berry size	520:543	average grape berry size at harvest	520:554	To address this issue, two vineyards from the same cultivar and subjected to the same physiological conditions during growing were selected for their difference in average grape berry size at harvest.
31646641	1	42	theme	red	180:182	arg1	wines					184:188	the best red wines	171:188	the best red wines	171:188	BACKGROUND A classical postulate of viticulture declares that small grape berries produce the best red wines.
31646641	2	43	theme	great	339:343	arg1	wines					349:353	great red wines	339:353	great red wines	339:353	The popularity of this postulate among winemakers leads them to consider berry size at harvest as a tool to measure the grape's potential to obtain great red wines.
31646641	5	44	theme	alcoholic	710:718	arg1	fermentation					720:731	the alcoholic fermentation	706:731	the alcoholic fermentation	706:731	Release of volatile compounds and phenolic compounds during the alcoholic fermentation was monitored, and the finished wines were chemically characterized.
31646641	3	45	theme	berry	534:538	arg1	size					540:543	average grape berry size	520:543	average grape berry size at harvest	520:554	To address this issue, two vineyards from the same cultivar and subjected to the same physiological conditions during growing were selected for their difference in average grape berry size at harvest.
31646641	2	46	theme	red	345:347	arg1	wines					349:353	great red wines	339:353	great red wines	339:353	The popularity of this postulate among winemakers leads them to consider berry size at harvest as a tool to measure the grape's potential to obtain great red wines.
31646641	0	47	theme	red	35:37	arg1	composition					44:54	red wine composition	35:54	red wine composition	35:54	Impact of berry size at harvest on red wine composition: a winemaker's approach.
31646641	9	48	theme	darker	1270:1275	arg1	wines					1281:1285	darker red wines	1270:1285	darker red wines	1270:1285	Thus not always smaller grapes produce darker red wines.
31646641	5	49	theme	phenolic	680:687	arg1	compounds					689:697	phenolic compounds	680:697	phenolic compounds	680:697	Release of volatile compounds and phenolic compounds during the alcoholic fermentation was monitored, and the finished wines were chemically characterized.
31646641	4	50	used	used	605:608	arg2	Grapes					557:562	Grapes	557:562	Grapes from both origins	557:580	Grapes from both origins were characterized and used for red winemaking by the same way.
31646641	4	51	theme	same	636:639	arg1	way					641:643	the same way	632:643	the same way	632:643	Grapes from both origins were characterized and used for red winemaking by the same way.
31646641	6	52	theme	theoretical	837:847	arg1	ratio					867:871	a lower theoretical surface-to-volume ratio	829:871	a lower theoretical surface-to-volume ratio	829:871	RESULTS Larger grapes have a lower theoretical surface-to-volume ratio but have thicker skins and a greater proportion of skins (m/m).
31646641	6	53	theme	thicker	882:888	arg1	skins					890:894	thicker skins	882:894	thicker skins	882:894	RESULTS Larger grapes have a lower theoretical surface-to-volume ratio but have thicker skins and a greater proportion of skins (m/m).
31646641	7	54	theme	greater	967:973	arg1	proportion					975:984	a greater proportion	965:984	a greater proportion of skins	965:993	Wines made from grapes with a greater proportion of skins contain higher amounts of phenolic compounds, terpenes, volatile acids, acetate esters and polysaccharides.
31646641	3	55	theme	average	520:526	arg1	size					540:543	average grape berry size	520:543	average grape berry size at harvest	520:554	To address this issue, two vineyards from the same cultivar and subjected to the same physiological conditions during growing were selected for their difference in average grape berry size at harvest.
31646641	8	56	theme	skin	1195:1198	arg1	proportion					1200:1209	skin proportion	1195:1209	skin proportion than to berry size	1195:1228	CONCLUSION According to the results, it seems that grape skin extraction is more related to skin proportion than to berry size.
31646641	6	57	dep	RESULTS	802:808	arg1	grapes					817:822	Larger grapes	810:822	RESULTS Larger grapes	802:822	RESULTS Larger grapes have a lower theoretical surface-to-volume ratio but have thicker skins and a greater proportion of skins (m/m).
31646641	7	58	theme	acids	1060:1064	arg1	terpenes					1041:1048	terpenes	1041:1048	terpenes	1041:1048	Wines made from grapes with a greater proportion of skins contain higher amounts of phenolic compounds, terpenes, volatile acids, acetate esters and polysaccharides.
31646641	7	58	theme	acids	1060:1064	arg1	esters					1075:1080	acetate esters	1067:1080	acetate esters	1067:1080	Wines made from grapes with a greater proportion of skins contain higher amounts of phenolic compounds, terpenes, volatile acids, acetate esters and polysaccharides.
31646641	7	58	theme	acids	1060:1064	arg1	polysaccharides					1086:1100	polysaccharides	1086:1100	polysaccharides	1086:1100	Wines made from grapes with a greater proportion of skins contain higher amounts of phenolic compounds, terpenes, volatile acids, acetate esters and polysaccharides.
31646641	7	58	theme	acids	1060:1064	arg1	amounts					1010:1016	higher amounts	1003:1016	higher amounts of phenolic compounds, terpenes, volatile acids, acetate esters and polysaccharides	1003:1100	Wines made from grapes with a greater proportion of skins contain higher amounts of phenolic compounds, terpenes, volatile acids, acetate esters and polysaccharides.
31646641	7	58	theme	acids	1060:1064	arg1	acids					1060:1064	volatile acids	1051:1064	volatile acids	1051:1064	Wines made from grapes with a greater proportion of skins contain higher amounts of phenolic compounds, terpenes, volatile acids, acetate esters and polysaccharides.
31646641	7	58	theme	acids	1060:1064	arg1	compounds					1030:1038	phenolic compounds	1021:1038	phenolic compounds	1021:1038	Wines made from grapes with a greater proportion of skins contain higher amounts of phenolic compounds, terpenes, volatile acids, acetate esters and polysaccharides.
31646641	6	59	contain	have	877:880	arg2	proportion					910:919	a greater proportion	900:919	a greater proportion of skins (m/m)	900:934	RESULTS Larger grapes have a lower theoretical surface-to-volume ratio but have thicker skins and a greater proportion of skins (m/m).
31646641	6	59	contain	have	877:880	arg1	RESULTS					802:808	RESULTS	802:808	RESULTS Larger grapes	802:822	RESULTS Larger grapes have a lower theoretical surface-to-volume ratio but have thicker skins and a greater proportion of skins (m/m).
31646641	6	59	contain	have	877:880	arg2	skins					890:894	thicker skins	882:894	thicker skins	882:894	RESULTS Larger grapes have a lower theoretical surface-to-volume ratio but have thicker skins and a greater proportion of skins (m/m).
31646641	3	60	dep	two	379:381	arg1	vineyards					383:391	vineyards	383:391	vineyards	383:391	To address this issue, two vineyards from the same cultivar and subjected to the same physiological conditions during growing were selected for their difference in average grape berry size at harvest.
31646641	3	60	dep	two	379:381	arg1	subjected					420:428	subjected	420:428	subjected to the same physiological conditions during growing	420:480	To address this issue, two vineyards from the same cultivar and subjected to the same physiological conditions during growing were selected for their difference in average grape berry size at harvest.
31646641	9	61	theme	smaller	1247:1253	arg1	grapes					1255:1260	Thus not always smaller grapes	1231:1260	Thus not always smaller grapes	1231:1260	Thus not always smaller grapes produce darker red wines.
31646641	3	62	theme	same	402:405	arg1	cultivar					407:414	the same cultivar	398:414	the same cultivar	398:414	To address this issue, two vineyards from the same cultivar and subjected to the same physiological conditions during growing were selected for their difference in average grape berry size at harvest.
31646641	8	63	dep	CONCLUSION	1103:1112	arg1	results					1131:1137	the results	1127:1137	the results	1127:1137	CONCLUSION According to the results, it seems that grape skin extraction is more related to skin proportion than to berry size.
31646641	3	64	theme	physiological	442:454	arg1	conditions					456:465	the same physiological conditions	433:465	the same physiological conditions	433:465	To address this issue, two vineyards from the same cultivar and subjected to the same physiological conditions during growing were selected for their difference in average grape berry size at harvest.
31646641	2	65	theme	postulate	214:222	arg1	popularity					195:204	The popularity	191:204	The popularity of this postulate among winemakers	191:239	The popularity of this postulate among winemakers leads them to consider berry size at harvest as a tool to measure the grape's potential to obtain great red wines.
31646641	3	66	theme	same	437:440	arg1	conditions					456:465	the same physiological conditions	433:465	the same physiological conditions	433:465	To address this issue, two vineyards from the same cultivar and subjected to the same physiological conditions during growing were selected for their difference in average grape berry size at harvest.
31646641	3	67	from	difference	506:515	arg1	harvest					548:554	harvest	548:554	harvest	548:554	To address this issue, two vineyards from the same cultivar and subjected to the same physiological conditions during growing were selected for their difference in average grape berry size at harvest.
31646641	3	67	from	difference	506:515	arg1	size					540:543	average grape berry size	520:543	average grape berry size at harvest	520:554	To address this issue, two vineyards from the same cultivar and subjected to the same physiological conditions during growing were selected for their difference in average grape berry size at harvest.
31646641	0	68	theme	wine	39:42	arg1	composition					44:54	red wine composition	35:54	red wine composition	35:54	Impact of berry size at harvest on red wine composition: a winemaker's approach.
31646641	9	69	dep	always	1240:1245	arg1	not					1236:1238	not	1236:1238	not	1236:1238	Thus not always smaller grapes produce darker red wines.
31646641	1	70	theme	viticulture	117:127	arg1	classical					94:102	classical	94:102	classical	94:102	BACKGROUND A classical postulate of viticulture declares that small grape berries produce the best red wines.
31007724	8	0	theme	S/G	1404:1406	arg1	ratios					1408:1413	their S/G ratios	1398:1413	their S/G ratios	1398:1413	Lignin polymers isolated from stems varied in their S/G ratios and linkage type distributions across genotypes.
31007724	11	1	theme	acid	1756:1759	arg1	Contents					1719:1726	Contents	1719:1726	Contents of p-coumaric acid, ferulic acid, and ferulic acid dimers	1719:1784	Contents of p-coumaric acid, ferulic acid, and ferulic acid dimers varied depending on genotype and organ type.
31007724	7	2	theme	highest	1306:1312	arg1	content					1343:1349	acetylbromide soluble lignin content	1314:1349	the highest acetylbromide soluble lignin content	1302:1349	Considering the same organs, the four genotypes did not differ in their Klason lignin contents, but Miscanthus × giganteus showed the highest acetylbromide soluble lignin content.
31007724	10	3	theme	8-5-coupled	1626:1636	arg1	acid					1648:1651	8-5-coupled diferulic acid	1626:1651	8-5-coupled diferulic acid being the main dimer, followed by 8-O-4-,	1626:1693	Ferulic acid dehydrodimers were analyzed as cell wall cross-links, with 8-5-coupled diferulic acid being the main dimer, followed by 8-O-4-, and 5-5-diferulic acid.
31007724	1	4	theme	plant	243:247	arg1	cell					249:252	the individual plant cell wall polymers and their interactions	228:289	cell	249:252	Efficient utilization of lignocellulosic Miscanthus biomass for the production of biochemicals, such as ethanol, is challenging due to its recalcitrance, which is influenced by the individual plant cell wall polymers and their interactions.
31007724	9	5	from	monomer	1530:1536	arg1	samples					1545:1551	all samples	1541:1551	all samples	1541:1551	p-Coumaric acid was the most abundant ester-bound hydroxycinnamte monomer in all samples.
31007724	7	6	theme	same	1188:1191	arg1	organs					1193:1198	the same organs	1184:1198	the same organs	1184:1198	Considering the same organs, the four genotypes did not differ in their Klason lignin contents, but Miscanthus × giganteus showed the highest acetylbromide soluble lignin content.
31007724	4	7	theme	cellulose	897:905	arg1	arabinoxylans					927:939	cellulose and low-substituted arabinoxylans	897:939	cellulose and low-substituted arabinoxylans	897:939	Polysaccharides of all genotypes are mainly composed of cellulose and low-substituted arabinoxylans.
31007724	5	8	theme	hemicelluloses	952:965	arg1	Ratios					942:947	Ratios	942:947	Ratios of hemicelluloses to cellulose	942:978	Ratios of hemicelluloses to cellulose were comparable, with the exception of Miscanthus sinensis that showed a higher hemicellulose/cellulose ratio.
31007724	10	9	theme	main	1663:1666	arg1	dimer					1668:1672	the main dimer	1659:1672	the main dimer	1659:1672	Ferulic acid dehydrodimers were analyzed as cell wall cross-links, with 8-5-coupled diferulic acid being the main dimer, followed by 8-O-4-, and 5-5-diferulic acid.
31007724	9	10	theme	ester-bound	1502:1512	arg1	acid					1475:1478	p-Coumaric acid	1464:1478	p-Coumaric acid	1464:1478	p-Coumaric acid was the most abundant ester-bound hydroxycinnamte monomer in all samples.
31007724	9	10	theme	ester-bound	1502:1512	arg1	monomer					1530:1536	the most abundant ester-bound hydroxycinnamte monomer	1484:1536	the most abundant ester-bound hydroxycinnamte monomer	1484:1536	p-Coumaric acid was the most abundant ester-bound hydroxycinnamte monomer in all samples.
31007724	3	11	dep	profiles	789:796	arg1	monomers					799:806	monomers	799:806	monomers	799:806	Here, four selected Miscanthus genotypes (Miscanthus sinensis, Miscanthus sacchariflorus, Miscanthus × giganteus, Miscanthus sinensis × Miscanthus sacchariflorus hybrid) were grown and harvested, separated into stems and leaves, and characterized for their non-starch polysaccharide composition and structures, lignin contents and structures, and hydroxycinnamate profiles (monomers and ferulic acid dehydrodimers).
31007724	3	11	dep	profiles	789:796	arg1	dehydrodimers					825:837	ferulic acid dehydrodimers	812:837	ferulic acid dehydrodimers	812:837	Here, four selected Miscanthus genotypes (Miscanthus sinensis, Miscanthus sacchariflorus, Miscanthus × giganteus, Miscanthus sinensis × Miscanthus sacchariflorus hybrid) were grown and harvested, separated into stems and leaves, and characterized for their non-starch polysaccharide composition and structures, lignin contents and structures, and hydroxycinnamate profiles (monomers and ferulic acid dehydrodimers).
31007724	12	12	theme	cell	1853:1856	arg1	cross-links					1863:1873	cell wall cross-links	1853:1873	cell wall cross-links	1853:1873	The largest amount of cell wall cross-links was analyzed for Miscanthus sinensis.
31007724	3	13	theme	Miscanthus	539:548	arg1	hybrid					587:592	Miscanthus sinensis × Miscanthus sacchariflorus hybrid	539:592	Miscanthus sinensis × Miscanthus sacchariflorus hybrid	539:592	Here, four selected Miscanthus genotypes (Miscanthus sinensis, Miscanthus sacchariflorus, Miscanthus × giganteus, Miscanthus sinensis × Miscanthus sacchariflorus hybrid) were grown and harvested, separated into stems and leaves, and characterized for their non-starch polysaccharide composition and structures, lignin contents and structures, and hydroxycinnamate profiles (monomers and ferulic acid dehydrodimers).
31007724	1	14	theme	wall	254:257	arg1	polymers					259:266	wall polymers	254:266	wall polymers	254:266	Efficient utilization of lignocellulosic Miscanthus biomass for the production of biochemicals, such as ethanol, is challenging due to its recalcitrance, which is influenced by the individual plant cell wall polymers and their interactions.
31007724	4	15	theme	low-substituted	911:925	arg1	arabinoxylans					927:939	cellulose and low-substituted arabinoxylans	897:939	cellulose and low-substituted arabinoxylans	897:939	Polysaccharides of all genotypes are mainly composed of cellulose and low-substituted arabinoxylans.
31007724	7	16	theme	acetylbromide	1314:1326	arg1	content					1343:1349	acetylbromide soluble lignin content	1314:1349	the highest acetylbromide soluble lignin content	1302:1349	Considering the same organs, the four genotypes did not differ in their Klason lignin contents, but Miscanthus × giganteus showed the highest acetylbromide soluble lignin content.
31007724	3	17	theme	sinensis × Miscanthus	550:570	arg1	hybrid					587:592	Miscanthus sinensis × Miscanthus sacchariflorus hybrid	539:592	Miscanthus sinensis × Miscanthus sacchariflorus hybrid	539:592	Here, four selected Miscanthus genotypes (Miscanthus sinensis, Miscanthus sacchariflorus, Miscanthus × giganteus, Miscanthus sinensis × Miscanthus sacchariflorus hybrid) were grown and harvested, separated into stems and leaves, and characterized for their non-starch polysaccharide composition and structures, lignin contents and structures, and hydroxycinnamate profiles (monomers and ferulic acid dehydrodimers).
31007724	7	18	theme	Klason	1244:1249	arg1	contents					1258:1265	their Klason lignin contents	1238:1265	their Klason lignin contents	1238:1265	Considering the same organs, the four genotypes did not differ in their Klason lignin contents, but Miscanthus × giganteus showed the highest acetylbromide soluble lignin content.
31007724	7	19	theme	soluble	1328:1334	arg1	content					1343:1349	acetylbromide soluble lignin content	1314:1349	the highest acetylbromide soluble lignin content	1302:1349	Considering the same organs, the four genotypes did not differ in their Klason lignin contents, but Miscanthus × giganteus showed the highest acetylbromide soluble lignin content.
31007724	6	20	theme	stems	1121:1125	arg1	contents					1098:1105	Lignin contents	1091:1105	Lignin contents of Miscanthus stems	1091:1125	Lignin contents of Miscanthus stems were higher than those of Miscanthus leaves.
31007724	1	21	theme	lignocellulosic	76:90	arg1	biomass					103:109	lignocellulosic Miscanthus biomass	76:109	lignocellulosic Miscanthus biomass	76:109	Efficient utilization of lignocellulosic Miscanthus biomass for the production of biochemicals, such as ethanol, is challenging due to its recalcitrance, which is influenced by the individual plant cell wall polymers and their interactions.
31007724	3	22	dep	grown	600:604	arg1	separated					621:629	separated	621:629	separated into stems	621:640	Here, four selected Miscanthus genotypes (Miscanthus sinensis, Miscanthus sacchariflorus, Miscanthus × giganteus, Miscanthus sinensis × Miscanthus sacchariflorus hybrid) were grown and harvested, separated into stems and leaves, and characterized for their non-starch polysaccharide composition and structures, lignin contents and structures, and hydroxycinnamate profiles (monomers and ferulic acid dehydrodimers).
31007724	7	23	theme	lignin	1251:1256	arg1	contents					1258:1265	their Klason lignin contents	1238:1265	their Klason lignin contents	1238:1265	Considering the same organs, the four genotypes did not differ in their Klason lignin contents, but Miscanthus × giganteus showed the highest acetylbromide soluble lignin content.
31007724	3	24	theme	lignin	736:741	arg1	contents					743:750	lignin contents	736:750	lignin contents	736:750	Here, four selected Miscanthus genotypes (Miscanthus sinensis, Miscanthus sacchariflorus, Miscanthus × giganteus, Miscanthus sinensis × Miscanthus sacchariflorus hybrid) were grown and harvested, separated into stems and leaves, and characterized for their non-starch polysaccharide composition and structures, lignin contents and structures, and hydroxycinnamate profiles (monomers and ferulic acid dehydrodimers).
31007724	5	25	with	comparable	985:994	arg1	exception					1006:1014	the exception	1002:1014	the exception of Miscanthus sinensis that showed a higher hemicellulose/cellulose ratio	1002:1088	Ratios of hemicelluloses to cellulose were comparable, with the exception of Miscanthus sinensis that showed a higher hemicellulose/cellulose ratio.
31007724	1	26	theme	Miscanthus	92:101	arg1	biomass					103:109	lignocellulosic Miscanthus biomass	76:109	lignocellulosic Miscanthus biomass	76:109	Efficient utilization of lignocellulosic Miscanthus biomass for the production of biochemicals, such as ethanol, is challenging due to its recalcitrance, which is influenced by the individual plant cell wall polymers and their interactions.
31007724	12	27	theme	Miscanthus	1892:1901	arg1	sinensis					1903:1910	Miscanthus sinensis	1892:1910	Miscanthus sinensis	1892:1910	The largest amount of cell wall cross-links was analyzed for Miscanthus sinensis.
31007724	2	28	theme	harvest	385:391	arg1	date					393:396	harvest date	385:396	harvest date	385:396	Lignocellulosic biomass composition differs depending on several factors, such as plant age, harvest date, organ type, and genotype.
31007724	10	29	theme	diferulic	1638:1646	arg1	acid					1648:1651	8-5-coupled diferulic acid	1626:1651	8-5-coupled diferulic acid being the main dimer, followed by 8-O-4-,	1626:1693	Ferulic acid dehydrodimers were analyzed as cell wall cross-links, with 8-5-coupled diferulic acid being the main dimer, followed by 8-O-4-, and 5-5-diferulic acid.
31007724	10	30	theme	acid	1562:1565	arg1	dehydrodimers					1567:1579	Ferulic acid dehydrodimers	1554:1579	Ferulic acid dehydrodimers	1554:1579	Ferulic acid dehydrodimers were analyzed as cell wall cross-links, with 8-5-coupled diferulic acid being the main dimer, followed by 8-O-4-, and 5-5-diferulic acid.
31007724	10	30	theme	acid	1562:1565	arg1	cross-links					1608:1618	cell wall cross-links	1598:1618	cell wall cross-links	1598:1618	Ferulic acid dehydrodimers were analyzed as cell wall cross-links, with 8-5-coupled diferulic acid being the main dimer, followed by 8-O-4-, and 5-5-diferulic acid.
31007724	1	31	theme	biomass	103:109	arg1	utilization					61:71	Efficient utilization	51:71	Efficient utilization of lignocellulosic Miscanthus biomass for the production of biochemicals, such as ethanol,	51:162	Efficient utilization of lignocellulosic Miscanthus biomass for the production of biochemicals, such as ethanol, is challenging due to its recalcitrance, which is influenced by the individual plant cell wall polymers and their interactions.
31007724	3	32	theme	hydroxycinnamate	772:787	arg1	profiles					789:796	hydroxycinnamate profiles	772:796	hydroxycinnamate profiles (monomers and ferulic acid dehydrodimers)	772:838	Here, four selected Miscanthus genotypes (Miscanthus sinensis, Miscanthus sacchariflorus, Miscanthus × giganteus, Miscanthus sinensis × Miscanthus sacchariflorus hybrid) were grown and harvested, separated into stems and leaves, and characterized for their non-starch polysaccharide composition and structures, lignin contents and structures, and hydroxycinnamate profiles (monomers and ferulic acid dehydrodimers).
31007724	10	33	theme	Ferulic	1554:1560	arg1	dehydrodimers					1567:1579	Ferulic acid dehydrodimers	1554:1579	Ferulic acid dehydrodimers	1554:1579	Ferulic acid dehydrodimers were analyzed as cell wall cross-links, with 8-5-coupled diferulic acid being the main dimer, followed by 8-O-4-, and 5-5-diferulic acid.
31007724	10	33	theme	Ferulic	1554:1560	arg1	cross-links					1608:1618	cell wall cross-links	1598:1618	cell wall cross-links	1598:1618	Ferulic acid dehydrodimers were analyzed as cell wall cross-links, with 8-5-coupled diferulic acid being the main dimer, followed by 8-O-4-, and 5-5-diferulic acid.
31007724	3	34	dep	Miscanthus	488:497	arg1	sacchariflorus					499:512	Miscanthus sacchariflorus	488:512	Miscanthus sacchariflorus	488:512	Here, four selected Miscanthus genotypes (Miscanthus sinensis, Miscanthus sacchariflorus, Miscanthus × giganteus, Miscanthus sinensis × Miscanthus sacchariflorus hybrid) were grown and harvested, separated into stems and leaves, and characterized for their non-starch polysaccharide composition and structures, lignin contents and structures, and hydroxycinnamate profiles (monomers and ferulic acid dehydrodimers).
31007724	0	35	theme	cell	31:34	arg1	polymers					41:48	Miscanthus cell wall polymers	20:48	Miscanthus cell wall polymers	20:48	Characterization of Miscanthus cell wall polymers.
31007724	8	36	attach	isolated	1368:1375	arg2	polymers					1359:1366	Lignin polymers	1352:1366	Lignin polymers isolated from stems	1352:1386	Lignin polymers isolated from stems varied in their S/G ratios and linkage type distributions across genotypes.
31007724	8	36	attach	isolated	1368:1375	arg1	stems					1382:1386	stems	1382:1386	stems	1382:1386	Lignin polymers isolated from stems varied in their S/G ratios and linkage type distributions across genotypes.
31007724	2	37	theme	biomass	308:314	arg1	composition					316:326	Lignocellulosic biomass composition	292:326	Lignocellulosic biomass composition	292:326	Lignocellulosic biomass composition differs depending on several factors, such as plant age, harvest date, organ type, and genotype.
31007724	3	38	theme	selected	436:443	arg1	Miscanthus					467:476	Miscanthus	467:476	Miscanthus	467:476	Here, four selected Miscanthus genotypes (Miscanthus sinensis, Miscanthus sacchariflorus, Miscanthus × giganteus, Miscanthus sinensis × Miscanthus sacchariflorus hybrid) were grown and harvested, separated into stems and leaves, and characterized for their non-starch polysaccharide composition and structures, lignin contents and structures, and hydroxycinnamate profiles (monomers and ferulic acid dehydrodimers).
31007724	3	38	theme	selected	436:443	arg1	genotypes					456:464	four selected Miscanthus genotypes	431:464	four selected Miscanthus genotypes (Miscanthus sinensis, Miscanthus sacchariflorus, Miscanthus × giganteus, Miscanthus sinensis × Miscanthus sacchariflorus hybrid)	431:593	Here, four selected Miscanthus genotypes (Miscanthus sinensis, Miscanthus sacchariflorus, Miscanthus × giganteus, Miscanthus sinensis × Miscanthus sacchariflorus hybrid) were grown and harvested, separated into stems and leaves, and characterized for their non-starch polysaccharide composition and structures, lignin contents and structures, and hydroxycinnamate profiles (monomers and ferulic acid dehydrodimers).
31007724	0	39	theme	Miscanthus	20:29	arg1	polymers					41:48	Miscanthus cell wall polymers	20:48	Miscanthus cell wall polymers	20:48	Characterization of Miscanthus cell wall polymers.
31007724	11	40	dep	acid	1742:1745	arg1	dimers					1779:1784	dimers	1779:1784	dimers	1779:1784	Contents of p-coumaric acid, ferulic acid, and ferulic acid dimers varied depending on genotype and organ type.
31007724	3	41	dep	Miscanthus	467:476	arg1	hybrid					587:592	Miscanthus sinensis × Miscanthus sacchariflorus hybrid	539:592	Miscanthus sinensis × Miscanthus sacchariflorus hybrid	539:592	Here, four selected Miscanthus genotypes (Miscanthus sinensis, Miscanthus sacchariflorus, Miscanthus × giganteus, Miscanthus sinensis × Miscanthus sacchariflorus hybrid) were grown and harvested, separated into stems and leaves, and characterized for their non-starch polysaccharide composition and structures, lignin contents and structures, and hydroxycinnamate profiles (monomers and ferulic acid dehydrodimers).
31007724	3	41	dep	Miscanthus	467:476	arg1	sinensis					478:485	Miscanthus sinensis	467:485	Miscanthus sinensis	467:485	Here, four selected Miscanthus genotypes (Miscanthus sinensis, Miscanthus sacchariflorus, Miscanthus × giganteus, Miscanthus sinensis × Miscanthus sacchariflorus hybrid) were grown and harvested, separated into stems and leaves, and characterized for their non-starch polysaccharide composition and structures, lignin contents and structures, and hydroxycinnamate profiles (monomers and ferulic acid dehydrodimers).
31007724	2	42	theme	Lignocellulosic	292:306	arg1	composition					316:326	Lignocellulosic biomass composition	292:326	Lignocellulosic biomass composition	292:326	Lignocellulosic biomass composition differs depending on several factors, such as plant age, harvest date, organ type, and genotype.
31007724	3	43	theme	Miscanthus	445:454	arg1	Miscanthus					467:476	Miscanthus	467:476	Miscanthus	467:476	Here, four selected Miscanthus genotypes (Miscanthus sinensis, Miscanthus sacchariflorus, Miscanthus × giganteus, Miscanthus sinensis × Miscanthus sacchariflorus hybrid) were grown and harvested, separated into stems and leaves, and characterized for their non-starch polysaccharide composition and structures, lignin contents and structures, and hydroxycinnamate profiles (monomers and ferulic acid dehydrodimers).
31007724	3	43	theme	Miscanthus	445:454	arg1	genotypes					456:464	four selected Miscanthus genotypes	431:464	four selected Miscanthus genotypes (Miscanthus sinensis, Miscanthus sacchariflorus, Miscanthus × giganteus, Miscanthus sinensis × Miscanthus sacchariflorus hybrid)	431:593	Here, four selected Miscanthus genotypes (Miscanthus sinensis, Miscanthus sacchariflorus, Miscanthus × giganteus, Miscanthus sinensis × Miscanthus sacchariflorus hybrid) were grown and harvested, separated into stems and leaves, and characterized for their non-starch polysaccharide composition and structures, lignin contents and structures, and hydroxycinnamate profiles (monomers and ferulic acid dehydrodimers).
31007724	0	44	theme	polymers	41:48	arg1	Characterization					0:15	Characterization	0:15	Characterization of Miscanthus cell wall polymers.	0:49	Characterization of Miscanthus cell wall polymers.
31007724	2	45	theme	several	349:355	arg1	type					405:408	organ type	399:408	organ type	399:408	Lignocellulosic biomass composition differs depending on several factors, such as plant age, harvest date, organ type, and genotype.
31007724	2	45	theme	several	349:355	arg1	age					380:382	plant age	374:382	plant age	374:382	Lignocellulosic biomass composition differs depending on several factors, such as plant age, harvest date, organ type, and genotype.
31007724	2	45	theme	several	349:355	arg1	factors					357:363	several factors	349:363	several factors	349:363	Lignocellulosic biomass composition differs depending on several factors, such as plant age, harvest date, organ type, and genotype.
31007724	2	45	theme	several	349:355	arg1	date					393:396	harvest date	385:396	harvest date	385:396	Lignocellulosic biomass composition differs depending on several factors, such as plant age, harvest date, organ type, and genotype.
31007724	2	45	theme	several	349:355	arg1	genotype					415:422	genotype	415:422	genotype	415:422	Lignocellulosic biomass composition differs depending on several factors, such as plant age, harvest date, organ type, and genotype.
31007724	6	46	theme	Miscanthus	1110:1119	arg1	stems					1121:1125	Miscanthus stems	1110:1125	Miscanthus stems	1110:1125	Lignin contents of Miscanthus stems were higher than those of Miscanthus leaves.
31007724	2	47	theme	organ	399:403	arg1	type					405:408	organ type	399:408	organ type	399:408	Lignocellulosic biomass composition differs depending on several factors, such as plant age, harvest date, organ type, and genotype.
31007724	0	48	theme	wall	36:39	arg1	polymers					41:48	Miscanthus cell wall polymers	20:48	Miscanthus cell wall polymers	20:48	Characterization of Miscanthus cell wall polymers.
31007724	9	49	theme	p-Coumaric	1464:1473	arg1	acid					1475:1478	p-Coumaric acid	1464:1478	p-Coumaric acid	1464:1478	p-Coumaric acid was the most abundant ester-bound hydroxycinnamte monomer in all samples.
31007724	9	49	theme	p-Coumaric	1464:1473	arg1	monomer					1530:1536	the most abundant ester-bound hydroxycinnamte monomer	1484:1536	the most abundant ester-bound hydroxycinnamte monomer	1484:1536	p-Coumaric acid was the most abundant ester-bound hydroxycinnamte monomer in all samples.
31007724	11	50	theme	p-coumaric	1731:1740	arg1	acid					1742:1745	p-coumaric acid	1731:1745	p-coumaric acid	1731:1745	Contents of p-coumaric acid, ferulic acid, and ferulic acid dimers varied depending on genotype and organ type.
31007724	6	51	dep	Miscanthus	1153:1162	arg1	leaves					1164:1169	leaves	1164:1169	leaves	1164:1169	Lignin contents of Miscanthus stems were higher than those of Miscanthus leaves.
31007724	9	52	theme	abundant	1493:1500	arg1	acid					1475:1478	p-Coumaric acid	1464:1478	p-Coumaric acid	1464:1478	p-Coumaric acid was the most abundant ester-bound hydroxycinnamte monomer in all samples.
31007724	9	52	theme	abundant	1493:1500	arg1	monomer					1530:1536	the most abundant ester-bound hydroxycinnamte monomer	1484:1536	the most abundant ester-bound hydroxycinnamte monomer	1484:1536	p-Coumaric acid was the most abundant ester-bound hydroxycinnamte monomer in all samples.
31007724	8	53	theme	Lignin	1352:1357	arg1	polymers					1359:1366	Lignin polymers	1352:1366	Lignin polymers isolated from stems	1352:1386	Lignin polymers isolated from stems varied in their S/G ratios and linkage type distributions across genotypes.
31007724	11	54	theme	ferulic	1748:1754	arg1	acid					1756:1759	ferulic acid	1748:1759	ferulic acid	1748:1759	Contents of p-coumaric acid, ferulic acid, and ferulic acid dimers varied depending on genotype and organ type.
31007724	3	55	theme	ferulic	812:818	arg1	dehydrodimers					825:837	ferulic acid dehydrodimers	812:837	ferulic acid dehydrodimers	812:837	Here, four selected Miscanthus genotypes (Miscanthus sinensis, Miscanthus sacchariflorus, Miscanthus × giganteus, Miscanthus sinensis × Miscanthus sacchariflorus hybrid) were grown and harvested, separated into stems and leaves, and characterized for their non-starch polysaccharide composition and structures, lignin contents and structures, and hydroxycinnamate profiles (monomers and ferulic acid dehydrodimers).
31007724	8	56	theme	linkage	1419:1425	arg1	distributions					1432:1444	linkage type distributions	1419:1444	linkage type distributions	1419:1444	Lignin polymers isolated from stems varied in their S/G ratios and linkage type distributions across genotypes.
31007724	11	57	theme	acid	1774:1777	arg1	Contents					1719:1726	Contents	1719:1726	Contents of p-coumaric acid, ferulic acid, and ferulic acid dimers	1719:1784	Contents of p-coumaric acid, ferulic acid, and ferulic acid dimers varied depending on genotype and organ type.
31007724	4	58	theme	genotypes	864:872	arg1	Polysaccharides					841:855	Polysaccharides	841:855	Polysaccharides of all genotypes	841:872	Polysaccharides of all genotypes are mainly composed of cellulose and low-substituted arabinoxylans.
31007724	11	59	theme	organ	1819:1823	arg1	type					1825:1828	organ type	1819:1828	organ type	1819:1828	Contents of p-coumaric acid, ferulic acid, and ferulic acid dimers varied depending on genotype and organ type.
31007724	10	60	theme	5-5-diferulic	1699:1711	arg1	acid					1713:1716	5-5-diferulic acid	1699:1716	5-5-diferulic acid	1699:1716	Ferulic acid dehydrodimers were analyzed as cell wall cross-links, with 8-5-coupled diferulic acid being the main dimer, followed by 8-O-4-, and 5-5-diferulic acid.
31007724	10	61	theme	wall	1603:1606	arg1	dehydrodimers					1567:1579	Ferulic acid dehydrodimers	1554:1579	Ferulic acid dehydrodimers	1554:1579	Ferulic acid dehydrodimers were analyzed as cell wall cross-links, with 8-5-coupled diferulic acid being the main dimer, followed by 8-O-4-, and 5-5-diferulic acid.
31007724	10	61	theme	wall	1603:1606	arg1	cross-links					1608:1618	cell wall cross-links	1598:1618	cell wall cross-links	1598:1618	Ferulic acid dehydrodimers were analyzed as cell wall cross-links, with 8-5-coupled diferulic acid being the main dimer, followed by 8-O-4-, and 5-5-diferulic acid.
31007724	3	62	theme	non-starch	682:691	arg1	composition					708:718	their non-starch polysaccharide composition	676:718	their non-starch polysaccharide composition	676:718	Here, four selected Miscanthus genotypes (Miscanthus sinensis, Miscanthus sacchariflorus, Miscanthus × giganteus, Miscanthus sinensis × Miscanthus sacchariflorus hybrid) were grown and harvested, separated into stems and leaves, and characterized for their non-starch polysaccharide composition and structures, lignin contents and structures, and hydroxycinnamate profiles (monomers and ferulic acid dehydrodimers).
31007724	1	63	theme	biochemicals	133:144	arg1	production					119:128	the production	115:128	the production of biochemicals, such as ethanol,	115:162	Efficient utilization of lignocellulosic Miscanthus biomass for the production of biochemicals, such as ethanol, is challenging due to its recalcitrance, which is influenced by the individual plant cell wall polymers and their interactions.
31007724	5	64	theme	hemicellulose/cellulose	1060:1082	arg1	ratio					1084:1088	a higher hemicellulose/cellulose ratio	1051:1088	a higher hemicellulose/cellulose ratio	1051:1088	Ratios of hemicelluloses to cellulose were comparable, with the exception of Miscanthus sinensis that showed a higher hemicellulose/cellulose ratio.
31007724	11	65	theme	ferulic	1766:1772	arg1	acid					1774:1777	ferulic acid	1766:1777	ferulic acid	1766:1777	Contents of p-coumaric acid, ferulic acid, and ferulic acid dimers varied depending on genotype and organ type.
31007724	3	66	theme	polysaccharide	693:706	arg1	composition					708:718	their non-starch polysaccharide composition	676:718	their non-starch polysaccharide composition	676:718	Here, four selected Miscanthus genotypes (Miscanthus sinensis, Miscanthus sacchariflorus, Miscanthus × giganteus, Miscanthus sinensis × Miscanthus sacchariflorus hybrid) were grown and harvested, separated into stems and leaves, and characterized for their non-starch polysaccharide composition and structures, lignin contents and structures, and hydroxycinnamate profiles (monomers and ferulic acid dehydrodimers).
31007724	12	67	theme	largest	1835:1841	arg1	amount					1843:1848	The largest amount	1831:1848	The largest amount of cell wall cross-links	1831:1873	The largest amount of cell wall cross-links was analyzed for Miscanthus sinensis.
31007724	12	67	theme	largest	1835:1841	arg1	cross-links					1863:1873	cell wall cross-links	1853:1873	cell wall cross-links	1853:1873	The largest amount of cell wall cross-links was analyzed for Miscanthus sinensis.
31007724	3	68	theme	sacchariflorus	572:585	arg1	hybrid					587:592	Miscanthus sinensis × Miscanthus sacchariflorus hybrid	539:592	Miscanthus sinensis × Miscanthus sacchariflorus hybrid	539:592	Here, four selected Miscanthus genotypes (Miscanthus sinensis, Miscanthus sacchariflorus, Miscanthus × giganteus, Miscanthus sinensis × Miscanthus sacchariflorus hybrid) were grown and harvested, separated into stems and leaves, and characterized for their non-starch polysaccharide composition and structures, lignin contents and structures, and hydroxycinnamate profiles (monomers and ferulic acid dehydrodimers).
31007724	8	69	theme	type	1427:1430	arg1	distributions					1432:1444	linkage type distributions	1419:1444	linkage type distributions	1419:1444	Lignin polymers isolated from stems varied in their S/G ratios and linkage type distributions across genotypes.
31007724	5	70	theme	sinensis	1030:1037	arg1	exception					1006:1014	the exception	1002:1014	the exception of Miscanthus sinensis that showed a higher hemicellulose/cellulose ratio	1002:1088	Ratios of hemicelluloses to cellulose were comparable, with the exception of Miscanthus sinensis that showed a higher hemicellulose/cellulose ratio.
31007724	12	71	theme	cross-links	1863:1873	arg1	amount					1843:1848	The largest amount	1831:1848	The largest amount of cell wall cross-links	1831:1873	The largest amount of cell wall cross-links was analyzed for Miscanthus sinensis.
31007724	12	71	theme	cross-links	1863:1873	arg1	cross-links					1863:1873	cell wall cross-links	1853:1873	cell wall cross-links	1853:1873	The largest amount of cell wall cross-links was analyzed for Miscanthus sinensis.
31007724	6	72	theme	Lignin	1091:1096	arg1	contents					1098:1105	Lignin contents	1091:1105	Lignin contents of Miscanthus stems	1091:1125	Lignin contents of Miscanthus stems were higher than those of Miscanthus leaves.
31007724	10	73	theme	cell	1598:1601	arg1	dehydrodimers					1567:1579	Ferulic acid dehydrodimers	1554:1579	Ferulic acid dehydrodimers	1554:1579	Ferulic acid dehydrodimers were analyzed as cell wall cross-links, with 8-5-coupled diferulic acid being the main dimer, followed by 8-O-4-, and 5-5-diferulic acid.
31007724	10	73	theme	cell	1598:1601	arg1	cross-links					1608:1618	cell wall cross-links	1598:1618	cell wall cross-links	1598:1618	Ferulic acid dehydrodimers were analyzed as cell wall cross-links, with 8-5-coupled diferulic acid being the main dimer, followed by 8-O-4-, and 5-5-diferulic acid.
31007724	1	74	dep	cell	249:252	arg1	polymers					259:266	wall polymers	254:266	wall polymers	254:266	Efficient utilization of lignocellulosic Miscanthus biomass for the production of biochemicals, such as ethanol, is challenging due to its recalcitrance, which is influenced by the individual plant cell wall polymers and their interactions.
31007724	5	75	theme	higher	1053:1058	arg1	ratio					1084:1088	a higher hemicellulose/cellulose ratio	1051:1088	a higher hemicellulose/cellulose ratio	1051:1088	Ratios of hemicelluloses to cellulose were comparable, with the exception of Miscanthus sinensis that showed a higher hemicellulose/cellulose ratio.
31007724	12	76	theme	wall	1858:1861	arg1	cross-links					1863:1873	cell wall cross-links	1853:1873	cell wall cross-links	1853:1873	The largest amount of cell wall cross-links was analyzed for Miscanthus sinensis.
31007724	2	77	theme	plant	374:378	arg1	age					380:382	plant age	374:382	plant age	374:382	Lignocellulosic biomass composition differs depending on several factors, such as plant age, harvest date, organ type, and genotype.
31007724	3	78	theme	acid	820:823	arg1	dehydrodimers					825:837	ferulic acid dehydrodimers	812:837	ferulic acid dehydrodimers	812:837	Here, four selected Miscanthus genotypes (Miscanthus sinensis, Miscanthus sacchariflorus, Miscanthus × giganteus, Miscanthus sinensis × Miscanthus sacchariflorus hybrid) were grown and harvested, separated into stems and leaves, and characterized for their non-starch polysaccharide composition and structures, lignin contents and structures, and hydroxycinnamate profiles (monomers and ferulic acid dehydrodimers).
31007724	9	79	theme	hydroxycinnamte	1514:1528	arg1	acid					1475:1478	p-Coumaric acid	1464:1478	p-Coumaric acid	1464:1478	p-Coumaric acid was the most abundant ester-bound hydroxycinnamte monomer in all samples.
31007724	9	79	theme	hydroxycinnamte	1514:1528	arg1	monomer					1530:1536	the most abundant ester-bound hydroxycinnamte monomer	1484:1536	the most abundant ester-bound hydroxycinnamte monomer	1484:1536	p-Coumaric acid was the most abundant ester-bound hydroxycinnamte monomer in all samples.
31007724	11	80	theme	acid	1742:1745	arg1	Contents					1719:1726	Contents	1719:1726	Contents of p-coumaric acid, ferulic acid, and ferulic acid dimers	1719:1784	Contents of p-coumaric acid, ferulic acid, and ferulic acid dimers varied depending on genotype and organ type.
31007724	1	81	theme	Efficient	51:59	arg1	utilization					61:71	Efficient utilization	51:71	Efficient utilization of lignocellulosic Miscanthus biomass for the production of biochemicals, such as ethanol,	51:162	Efficient utilization of lignocellulosic Miscanthus biomass for the production of biochemicals, such as ethanol, is challenging due to its recalcitrance, which is influenced by the individual plant cell wall polymers and their interactions.
31007724	7	82	theme	lignin	1336:1341	arg1	content					1343:1349	acetylbromide soluble lignin content	1314:1349	the highest acetylbromide soluble lignin content	1302:1349	Considering the same organs, the four genotypes did not differ in their Klason lignin contents, but Miscanthus × giganteus showed the highest acetylbromide soluble lignin content.
31007724	1	83	theme	individual	232:241	arg1	cell					249:252	the individual plant cell wall polymers and their interactions	228:289	cell	249:252	Efficient utilization of lignocellulosic Miscanthus biomass for the production of biochemicals, such as ethanol, is challenging due to its recalcitrance, which is influenced by the individual plant cell wall polymers and their interactions.
30528936	8	0	theme	antidepressant	1548:1561	arg1	effects					1563:1569	the antidepressant effects	1544:1569	the antidepressant effects of ketamine	1544:1581	Importantly, the receiver operating characteristic (ROC) curves demonstrated that the phylum Actinobacteria and the class Coriobacteriia were potential biomarker for the antidepressant effects of ketamine in an inflammation model.
30528936	1	1	theme	acid	157:160	arg1	NMDAR					172:176	NMDAR	172:176	NMDAR	172:176	Ketamine, an N-methyl-d-aspartic acid receptor (NMDAR) antagonist, elicits rapid-acting and sustained antidepressant effects in treatment-resistant depressed patients.
30528936	1	1	theme	acid	157:160	arg1	receptor					162:169	N-methyl-d-aspartic acid receptor	137:169	an N-methyl-d-aspartic acid receptor (NMDAR) antagonist	134:188	Ketamine, an N-methyl-d-aspartic acid receptor (NMDAR) antagonist, elicits rapid-acting and sustained antidepressant effects in treatment-resistant depressed patients.
30528936	1	2	theme	treatment-resistant	252:270	arg1	patients					282:289	treatment-resistant depressed patients	252:289	treatment-resistant depressed patients	252:289	Ketamine, an N-methyl-d-aspartic acid receptor (NMDAR) antagonist, elicits rapid-acting and sustained antidepressant effects in treatment-resistant depressed patients.
30528936	0	3	theme	ketamine	75:82	arg1	effects					64:70	the antidepressant effects	45:70	the antidepressant effects of ketamine in an inflammation model of depression	45:121	Role of Actinobacteria and Coriobacteriia in the antidepressant effects of ketamine in an inflammation model of depression.
30528936	1	4	theme	receptor	162:169	arg1	Ketamine					124:131	Ketamine	124:131	Ketamine	124:131	Ketamine, an N-methyl-d-aspartic acid receptor (NMDAR) antagonist, elicits rapid-acting and sustained antidepressant effects in treatment-resistant depressed patients.
30528936	1	4	theme	receptor	162:169	arg1	antagonist					179:188	an N-methyl-d-aspartic acid receptor (NMDAR) antagonist	134:188	an N-methyl-d-aspartic acid receptor (NMDAR) antagonist	134:188	Ketamine, an N-methyl-d-aspartic acid receptor (NMDAR) antagonist, elicits rapid-acting and sustained antidepressant effects in treatment-resistant depressed patients.
30528936	1	5	theme	depressed	272:280	arg1	patients					282:289	treatment-resistant depressed patients	252:289	treatment-resistant depressed patients	252:289	Ketamine, an N-methyl-d-aspartic acid receptor (NMDAR) antagonist, elicits rapid-acting and sustained antidepressant effects in treatment-resistant depressed patients.
30528936	0	6	theme	inflammation	90:101	arg1	model					103:107	an inflammation model	87:107	an inflammation model of depression	87:121	Role of Actinobacteria and Coriobacteriia in the antidepressant effects of ketamine in an inflammation model of depression.
30528936	0	7	from	Role	0:3	arg1	effects					64:70	the antidepressant effects	45:70	the antidepressant effects of ketamine in an inflammation model of depression	45:121	Role of Actinobacteria and Coriobacteriia in the antidepressant effects of ketamine in an inflammation model of depression.
30528936	7	8	theme	FST	1373:1375	arg1	time					1365:1368	the immobility time	1350:1368	the immobility time of FST	1350:1375	Interestingly, two bacteria, including the phylum Actinobacteria and the class Coriobacteriia were significantly correlated with the immobility time of FST.
30528936	3	9	theme	ketamine	567:574	arg1	effects					556:562	the antidepressant effects	537:562	the antidepressant effects of ketamine in lipopolysaccharide (LPS)-induced inflammation model of depression	537:643	Here we investigated the role of gut microbiota in the antidepressant effects of ketamine in lipopolysaccharide (LPS)-induced inflammation model of depression.
30528936	5	10	theme	ketamine	1072:1079	arg1	treatment					1081:1089	ketamine treatment	1072:1089	ketamine treatment	1072:1089	In addition to α-diversity, β-diversity, such as principal coordinates analysis (PCoA), and linear discriminant analysis (LDA) coupled with effect size measurements (LEfSe), showed a differential profile after ketamine treatment.
30528936	5	11	theme	discriminant	961:972	arg1	LDA					984:986	LDA	984:986	LDA	984:986	In addition to α-diversity, β-diversity, such as principal coordinates analysis (PCoA), and linear discriminant analysis (LDA) coupled with effect size measurements (LEfSe), showed a differential profile after ketamine treatment.
30528936	5	11	theme	discriminant	961:972	arg1	analysis					974:981	linear discriminant analysis	954:981	linear discriminant analysis (LDA) coupled with effect size measurements (LEfSe)	954:1033	In addition to α-diversity, β-diversity, such as principal coordinates analysis (PCoA), and linear discriminant analysis (LDA) coupled with effect size measurements (LEfSe), showed a differential profile after ketamine treatment.
30528936	4	12	from	improvements	782:793	arg1	α-diversity					798:808	α-diversity	798:808	α-diversity	798:808	Ketamine (10 mg/kg) significantly attenuated the increased immobility time in forced swimming test (FST), which was associated with the improvements in α-diversity, consisting of Shannon, Simpson and Chao 1 indices.
30528936	3	13	theme	microbiota	523:532	arg1	role					511:514	the role	507:514	the role of gut microbiota in the antidepressant effects of ketamine in lipopolysaccharide (LPS)-induced inflammation model of depression	507:643	Here we investigated the role of gut microbiota in the antidepressant effects of ketamine in lipopolysaccharide (LPS)-induced inflammation model of depression.
30528936	9	14	theme	class	1793:1797	arg1	biomarkers					1833:1842	potential biomarkers	1823:1842	potential biomarkers for ketamine's antidepressant efficacy	1823:1881	These findings suggest that antidepressant effects of ketamine might be related to the regulation of abnormal composition of gut microbiota, and that the phylum Actinobacteria and the class Coriobacteriia might be potential biomarkers for ketamine's antidepressant efficacy.
30528936	9	14	theme	class	1793:1797	arg1	Coriobacteriia					1799:1812	the class Coriobacteriia	1789:1812	the class Coriobacteriia	1789:1812	These findings suggest that antidepressant effects of ketamine might be related to the regulation of abnormal composition of gut microbiota, and that the phylum Actinobacteria and the class Coriobacteriia might be potential biomarkers for ketamine's antidepressant efficacy.
30528936	9	15	theme	gut	1734:1736	arg1	microbiota					1738:1747	gut microbiota	1734:1747	gut microbiota	1734:1747	These findings suggest that antidepressant effects of ketamine might be related to the regulation of abnormal composition of gut microbiota, and that the phylum Actinobacteria and the class Coriobacteriia might be potential biomarkers for ketamine's antidepressant efficacy.
30528936	4	16	theme	swimming	731:738	arg1	FST					746:748	FST	746:748	FST	746:748	Ketamine (10 mg/kg) significantly attenuated the increased immobility time in forced swimming test (FST), which was associated with the improvements in α-diversity, consisting of Shannon, Simpson and Chao 1 indices.
30528936	4	16	theme	swimming	731:738	arg1	test					740:743	forced swimming test	724:743	forced swimming test (FST)	724:749	Ketamine (10 mg/kg) significantly attenuated the increased immobility time in forced swimming test (FST), which was associated with the improvements in α-diversity, consisting of Shannon, Simpson and Chao 1 indices.
30528936	7	17	theme	phylum	1264:1269	arg1	Actinobacteria					1271:1284	the phylum Actinobacteria	1260:1284	the phylum Actinobacteria	1260:1284	Interestingly, two bacteria, including the phylum Actinobacteria and the class Coriobacteriia were significantly correlated with the immobility time of FST.
30528936	4	18	theme	increased	695:703	arg1	time					716:719	the increased immobility time	691:719	the increased immobility time	691:719	Ketamine (10 mg/kg) significantly attenuated the increased immobility time in forced swimming test (FST), which was associated with the improvements in α-diversity, consisting of Shannon, Simpson and Chao 1 indices.
30528936	9	19	theme	potential	1823:1831	arg1	biomarkers					1833:1842	potential biomarkers	1823:1842	potential biomarkers for ketamine's antidepressant efficacy	1823:1881	These findings suggest that antidepressant effects of ketamine might be related to the regulation of abnormal composition of gut microbiota, and that the phylum Actinobacteria and the class Coriobacteriia might be potential biomarkers for ketamine's antidepressant efficacy.
30528936	9	19	theme	potential	1823:1831	arg1	Actinobacteria					1770:1783	the phylum Actinobacteria	1759:1783	the phylum Actinobacteria	1759:1783	These findings suggest that antidepressant effects of ketamine might be related to the regulation of abnormal composition of gut microbiota, and that the phylum Actinobacteria and the class Coriobacteriia might be potential biomarkers for ketamine's antidepressant efficacy.
30528936	9	19	theme	potential	1823:1831	arg1	Coriobacteriia					1799:1812	the class Coriobacteriia	1789:1812	the class Coriobacteriia	1789:1812	These findings suggest that antidepressant effects of ketamine might be related to the regulation of abnormal composition of gut microbiota, and that the phylum Actinobacteria and the class Coriobacteriia might be potential biomarkers for ketamine's antidepressant efficacy.
30528936	2	20	theme	Accumulating	292:303	arg1	evidence					305:312	Accumulating evidence	292:312	Accumulating evidence	292:312	Accumulating evidence suggests that gut microbiota via the gut-brain axis play a role in the pathogenesis of depression, thereby contributing to the antidepressant actions of certain compounds.
30528936	5	21	dep	α-diversity	877:887	arg1	addition					865:872	addition	865:872	addition	865:872	In addition to α-diversity, β-diversity, such as principal coordinates analysis (PCoA), and linear discriminant analysis (LDA) coupled with effect size measurements (LEfSe), showed a differential profile after ketamine treatment.
30528936	9	22	theme	antidepressant	1637:1650	arg1	effects					1652:1658	antidepressant effects	1637:1658	antidepressant effects of ketamine	1637:1670	These findings suggest that antidepressant effects of ketamine might be related to the regulation of abnormal composition of gut microbiota, and that the phylum Actinobacteria and the class Coriobacteriia might be potential biomarkers for ketamine's antidepressant efficacy.
30528936	2	23	theme	compounds	475:483	arg1	actions					456:462	the antidepressant actions	437:462	the antidepressant actions of certain compounds	437:483	Accumulating evidence suggests that gut microbiota via the gut-brain axis play a role in the pathogenesis of depression, thereby contributing to the antidepressant actions of certain compounds.
30528936	3	24	theme	antidepressant	541:554	arg1	effects					556:562	the antidepressant effects	537:562	the antidepressant effects of ketamine in lipopolysaccharide (LPS)-induced inflammation model of depression	537:643	Here we investigated the role of gut microbiota in the antidepressant effects of ketamine in lipopolysaccharide (LPS)-induced inflammation model of depression.
30528936	9	25	theme	abnormal	1710:1717	arg1	composition					1719:1729	abnormal composition	1710:1729	abnormal composition of gut microbiota	1710:1747	These findings suggest that antidepressant effects of ketamine might be related to the regulation of abnormal composition of gut microbiota, and that the phylum Actinobacteria and the class Coriobacteriia might be potential biomarkers for ketamine's antidepressant efficacy.
30528936	3	26	from	role	511:514	arg1	effects					556:562	the antidepressant effects	537:562	the antidepressant effects of ketamine in lipopolysaccharide (LPS)-induced inflammation model of depression	537:643	Here we investigated the role of gut microbiota in the antidepressant effects of ketamine in lipopolysaccharide (LPS)-induced inflammation model of depression.
30528936	2	27	theme	depression	401:410	arg1	pathogenesis					385:396	the pathogenesis	381:396	the pathogenesis of depression	381:410	Accumulating evidence suggests that gut microbiota via the gut-brain axis play a role in the pathogenesis of depression, thereby contributing to the antidepressant actions of certain compounds.
30528936	2	28	theme	certain	467:473	arg1	compounds					475:483	certain compounds	467:483	certain compounds	467:483	Accumulating evidence suggests that gut microbiota via the gut-brain axis play a role in the pathogenesis of depression, thereby contributing to the antidepressant actions of certain compounds.
30528936	8	29	theme	ketamine	1574:1581	arg1	effects					1563:1569	the antidepressant effects	1544:1569	the antidepressant effects of ketamine	1544:1581	Importantly, the receiver operating characteristic (ROC) curves demonstrated that the phylum Actinobacteria and the class Coriobacteriia were potential biomarker for the antidepressant effects of ketamine in an inflammation model.
30528936	9	30	theme	composition	1719:1729	arg1	regulation					1696:1705	the regulation	1692:1705	the regulation of abnormal composition of gut microbiota	1692:1747	These findings suggest that antidepressant effects of ketamine might be related to the regulation of abnormal composition of gut microbiota, and that the phylum Actinobacteria and the class Coriobacteriia might be potential biomarkers for ketamine's antidepressant efficacy.
30528936	4	31	theme	immobility	705:714	arg1	time					716:719	the increased immobility time	691:719	the increased immobility time	691:719	Ketamine (10 mg/kg) significantly attenuated the increased immobility time in forced swimming test (FST), which was associated with the improvements in α-diversity, consisting of Shannon, Simpson and Chao 1 indices.
30528936	9	32	theme	ketamine	1663:1670	arg1	effects					1652:1658	antidepressant effects	1637:1658	antidepressant effects of ketamine	1637:1670	These findings suggest that antidepressant effects of ketamine might be related to the regulation of abnormal composition of gut microbiota, and that the phylum Actinobacteria and the class Coriobacteriia might be potential biomarkers for ketamine's antidepressant efficacy.
30528936	6	33	theme	treated	1207:1213	arg1	mice					1215:1218	LPS + ketamine treated mice	1192:1218	LPS + ketamine treated mice	1192:1218	Furthermore, a total of 30 bacteria were significantly altered in the LPS + saline treated mice and LPS + ketamine treated mice.
30528936	6	34	theme	bacteria	1119:1126	arg1	total					1107:1111	a total	1105:1111	a total of 30 bacteria	1105:1126	Furthermore, a total of 30 bacteria were significantly altered in the LPS + saline treated mice and LPS + ketamine treated mice.
30528936	5	35	theme	effect	1002:1007	arg1	LEfSe					1028:1032	LEfSe	1028:1032	LEfSe	1028:1032	In addition to α-diversity, β-diversity, such as principal coordinates analysis (PCoA), and linear discriminant analysis (LDA) coupled with effect size measurements (LEfSe), showed a differential profile after ketamine treatment.
30528936	5	35	theme	effect	1002:1007	arg1	measurements					1014:1025	effect size measurements	1002:1025	effect size measurements (LEfSe)	1002:1033	In addition to α-diversity, β-diversity, such as principal coordinates analysis (PCoA), and linear discriminant analysis (LDA) coupled with effect size measurements (LEfSe), showed a differential profile after ketamine treatment.
30528936	2	36	theme	antidepressant	441:454	arg1	actions					456:462	the antidepressant actions	437:462	the antidepressant actions of certain compounds	437:483	Accumulating evidence suggests that gut microbiota via the gut-brain axis play a role in the pathogenesis of depression, thereby contributing to the antidepressant actions of certain compounds.
30528936	0	37	theme	Actinobacteria	8:21	arg1	Role					0:3	Role	0:3	Role of Actinobacteria and Coriobacteriia in the antidepressant effects of ketamine in an inflammation model of depression.	0:122	Role of Actinobacteria and Coriobacteriia in the antidepressant effects of ketamine in an inflammation model of depression.
30528936	0	38	theme	depression	112:121	arg1	model					103:107	an inflammation model	87:107	an inflammation model of depression	87:121	Role of Actinobacteria and Coriobacteriia in the antidepressant effects of ketamine in an inflammation model of depression.
30528936	6	39	theme	LPS + saline	1162:1173	arg1	mice					1183:1186	the LPS + saline treated mice	1158:1186	the LPS + saline treated mice	1158:1186	Furthermore, a total of 30 bacteria were significantly altered in the LPS + saline treated mice and LPS + ketamine treated mice.
30528936	2	40	theme	gut	328:330	arg1	microbiota					332:341	gut microbiota	328:341	gut microbiota via the gut-brain axis	328:364	Accumulating evidence suggests that gut microbiota via the gut-brain axis play a role in the pathogenesis of depression, thereby contributing to the antidepressant actions of certain compounds.
30528936	8	41	dep	characteristic	1414:1427	arg1	ROC					1430:1432	ROC	1430:1432	ROC	1430:1432	Importantly, the receiver operating characteristic (ROC) curves demonstrated that the phylum Actinobacteria and the class Coriobacteriia were potential biomarker for the antidepressant effects of ketamine in an inflammation model.
30528936	9	42	theme	antidepressant	1859:1872	arg1	efficacy					1874:1881	ketamine's antidepressant efficacy	1848:1881	ketamine's antidepressant efficacy	1848:1881	These findings suggest that antidepressant effects of ketamine might be related to the regulation of abnormal composition of gut microbiota, and that the phylum Actinobacteria and the class Coriobacteriia might be potential biomarkers for ketamine's antidepressant efficacy.
30528936	5	43	theme	coordinates	921:931	arg1	PCoA					943:946	PCoA	943:946	PCoA	943:946	In addition to α-diversity, β-diversity, such as principal coordinates analysis (PCoA), and linear discriminant analysis (LDA) coupled with effect size measurements (LEfSe), showed a differential profile after ketamine treatment.
30528936	5	43	theme	coordinates	921:931	arg1	analysis					933:940	principal coordinates analysis	911:940	principal coordinates analysis (PCoA)	911:947	In addition to α-diversity, β-diversity, such as principal coordinates analysis (PCoA), and linear discriminant analysis (LDA) coupled with effect size measurements (LEfSe), showed a differential profile after ketamine treatment.
30528936	9	44	theme	microbiota	1738:1747	arg1	composition					1719:1729	abnormal composition	1710:1729	abnormal composition of gut microbiota	1710:1747	These findings suggest that antidepressant effects of ketamine might be related to the regulation of abnormal composition of gut microbiota, and that the phylum Actinobacteria and the class Coriobacteriia might be potential biomarkers for ketamine's antidepressant efficacy.
30528936	4	45	dep	Simpson	834:840	arg1	indices					853:859	indices	853:859	indices	853:859	Ketamine (10 mg/kg) significantly attenuated the increased immobility time in forced swimming test (FST), which was associated with the improvements in α-diversity, consisting of Shannon, Simpson and Chao 1 indices.
30528936	4	46	dep	attenuated	680:689	arg1	associated					762:771	associated	762:771	was associated with the improvements in α-diversity, consisting of Shannon, Simpson and Chao 1 indices	758:859	Ketamine (10 mg/kg) significantly attenuated the increased immobility time in forced swimming test (FST), which was associated with the improvements in α-diversity, consisting of Shannon, Simpson and Chao 1 indices.
30528936	1	47	theme	rapid-acting	199:210	arg1	effects					241:247	rapid-acting and sustained antidepressant effects	199:247	rapid-acting and sustained antidepressant effects	199:247	Ketamine, an N-methyl-d-aspartic acid receptor (NMDAR) antagonist, elicits rapid-acting and sustained antidepressant effects in treatment-resistant depressed patients.
30528936	0	48	theme	Coriobacteriia	27:40	arg1	Role					0:3	Role	0:3	Role of Actinobacteria and Coriobacteriia in the antidepressant effects of ketamine in an inflammation model of depression.	0:122	Role of Actinobacteria and Coriobacteriia in the antidepressant effects of ketamine in an inflammation model of depression.
30528936	8	49	from	biomarker	1530:1538	arg1	model					1602:1606	an inflammation model	1586:1606	an inflammation model	1586:1606	Importantly, the receiver operating characteristic (ROC) curves demonstrated that the phylum Actinobacteria and the class Coriobacteriia were potential biomarker for the antidepressant effects of ketamine in an inflammation model.
30528936	8	50	theme	phylum	1464:1469	arg1	biomarker					1530:1538	potential biomarker	1520:1538	potential biomarker for the antidepressant effects of ketamine	1520:1581	Importantly, the receiver operating characteristic (ROC) curves demonstrated that the phylum Actinobacteria and the class Coriobacteriia were potential biomarker for the antidepressant effects of ketamine in an inflammation model.
30528936	8	50	theme	phylum	1464:1469	arg1	Actinobacteria					1471:1484	the phylum Actinobacteria	1460:1484	the phylum Actinobacteria	1460:1484	Importantly, the receiver operating characteristic (ROC) curves demonstrated that the phylum Actinobacteria and the class Coriobacteriia were potential biomarker for the antidepressant effects of ketamine in an inflammation model.
30528936	5	51	theme	size	1009:1012	arg1	LEfSe					1028:1032	LEfSe	1028:1032	LEfSe	1028:1032	In addition to α-diversity, β-diversity, such as principal coordinates analysis (PCoA), and linear discriminant analysis (LDA) coupled with effect size measurements (LEfSe), showed a differential profile after ketamine treatment.
30528936	5	51	theme	size	1009:1012	arg1	measurements					1014:1025	effect size measurements	1002:1025	effect size measurements (LEfSe)	1002:1033	In addition to α-diversity, β-diversity, such as principal coordinates analysis (PCoA), and linear discriminant analysis (LDA) coupled with effect size measurements (LEfSe), showed a differential profile after ketamine treatment.
30528936	0	52	theme	antidepressant	49:62	arg1	effects					64:70	the antidepressant effects	45:70	the antidepressant effects of ketamine in an inflammation model of depression	45:121	Role of Actinobacteria and Coriobacteriia in the antidepressant effects of ketamine in an inflammation model of depression.
30528936	8	53	theme	inflammation	1589:1600	arg1	model					1602:1606	an inflammation model	1586:1606	an inflammation model	1586:1606	Importantly, the receiver operating characteristic (ROC) curves demonstrated that the phylum Actinobacteria and the class Coriobacteriia were potential biomarker for the antidepressant effects of ketamine in an inflammation model.
30528936	0	54	from	effects	64:70	arg1	model					103:107	an inflammation model	87:107	an inflammation model of depression	87:121	Role of Actinobacteria and Coriobacteriia in the antidepressant effects of ketamine in an inflammation model of depression.
30528936	3	55	theme	-induced	603:610	arg1	model					625:629	lipopolysaccharide (LPS)-induced inflammation model	579:629	lipopolysaccharide (LPS)-induced inflammation model of depression	579:643	Here we investigated the role of gut microbiota in the antidepressant effects of ketamine in lipopolysaccharide (LPS)-induced inflammation model of depression.
30528936	5	56	theme	principal	911:919	arg1	PCoA					943:946	PCoA	943:946	PCoA	943:946	In addition to α-diversity, β-diversity, such as principal coordinates analysis (PCoA), and linear discriminant analysis (LDA) coupled with effect size measurements (LEfSe), showed a differential profile after ketamine treatment.
30528936	5	56	theme	principal	911:919	arg1	analysis					933:940	principal coordinates analysis	911:940	principal coordinates analysis (PCoA)	911:947	In addition to α-diversity, β-diversity, such as principal coordinates analysis (PCoA), and linear discriminant analysis (LDA) coupled with effect size measurements (LEfSe), showed a differential profile after ketamine treatment.
30528936	8	57	theme	potential	1520:1528	arg1	Coriobacteriia					1500:1513	the class Coriobacteriia	1490:1513	the class Coriobacteriia	1490:1513	Importantly, the receiver operating characteristic (ROC) curves demonstrated that the phylum Actinobacteria and the class Coriobacteriia were potential biomarker for the antidepressant effects of ketamine in an inflammation model.
30528936	8	57	theme	potential	1520:1528	arg1	biomarker					1530:1538	potential biomarker	1520:1538	potential biomarker for the antidepressant effects of ketamine	1520:1581	Importantly, the receiver operating characteristic (ROC) curves demonstrated that the phylum Actinobacteria and the class Coriobacteriia were potential biomarker for the antidepressant effects of ketamine in an inflammation model.
30528936	8	57	theme	potential	1520:1528	arg1	Actinobacteria					1471:1484	the phylum Actinobacteria	1460:1484	the phylum Actinobacteria	1460:1484	Importantly, the receiver operating characteristic (ROC) curves demonstrated that the phylum Actinobacteria and the class Coriobacteriia were potential biomarker for the antidepressant effects of ketamine in an inflammation model.
30528936	8	58	theme	characteristic	1414:1427	arg1	curves					1435:1440	characteristic (ROC) curves	1414:1440	characteristic (ROC) curves	1414:1440	Importantly, the receiver operating characteristic (ROC) curves demonstrated that the phylum Actinobacteria and the class Coriobacteriia were potential biomarker for the antidepressant effects of ketamine in an inflammation model.
30528936	3	59	theme	inflammation	612:623	arg1	model					625:629	lipopolysaccharide (LPS)-induced inflammation model	579:629	lipopolysaccharide (LPS)-induced inflammation model of depression	579:643	Here we investigated the role of gut microbiota in the antidepressant effects of ketamine in lipopolysaccharide (LPS)-induced inflammation model of depression.
30528936	3	60	theme	gut	519:521	arg1	microbiota					523:532	gut microbiota	519:532	gut microbiota	519:532	Here we investigated the role of gut microbiota in the antidepressant effects of ketamine in lipopolysaccharide (LPS)-induced inflammation model of depression.
30528936	7	61	theme	immobility	1354:1363	arg1	time					1365:1368	the immobility time	1350:1368	the immobility time of FST	1350:1375	Interestingly, two bacteria, including the phylum Actinobacteria and the class Coriobacteriia were significantly correlated with the immobility time of FST.
30528936	6	62	theme	LPS + ketamine	1192:1205	arg1	mice					1215:1218	LPS + ketamine treated mice	1192:1218	LPS + ketamine treated mice	1192:1218	Furthermore, a total of 30 bacteria were significantly altered in the LPS + saline treated mice and LPS + ketamine treated mice.
30528936	1	63	theme	sustained	216:224	arg1	effects					241:247	rapid-acting and sustained antidepressant effects	199:247	rapid-acting and sustained antidepressant effects	199:247	Ketamine, an N-methyl-d-aspartic acid receptor (NMDAR) antagonist, elicits rapid-acting and sustained antidepressant effects in treatment-resistant depressed patients.
30528936	9	64	theme	phylum	1763:1768	arg1	Actinobacteria					1770:1783	the phylum Actinobacteria	1759:1783	the phylum Actinobacteria	1759:1783	These findings suggest that antidepressant effects of ketamine might be related to the regulation of abnormal composition of gut microbiota, and that the phylum Actinobacteria and the class Coriobacteriia might be potential biomarkers for ketamine's antidepressant efficacy.
30528936	9	64	theme	phylum	1763:1768	arg1	biomarkers					1833:1842	potential biomarkers	1823:1842	potential biomarkers for ketamine's antidepressant efficacy	1823:1881	These findings suggest that antidepressant effects of ketamine might be related to the regulation of abnormal composition of gut microbiota, and that the phylum Actinobacteria and the class Coriobacteriia might be potential biomarkers for ketamine's antidepressant efficacy.
30528936	8	65	theme	class	1494:1498	arg1	Coriobacteriia					1500:1513	the class Coriobacteriia	1490:1513	the class Coriobacteriia	1490:1513	Importantly, the receiver operating characteristic (ROC) curves demonstrated that the phylum Actinobacteria and the class Coriobacteriia were potential biomarker for the antidepressant effects of ketamine in an inflammation model.
30528936	8	65	theme	class	1494:1498	arg1	biomarker					1530:1538	potential biomarker	1520:1538	potential biomarker for the antidepressant effects of ketamine	1520:1581	Importantly, the receiver operating characteristic (ROC) curves demonstrated that the phylum Actinobacteria and the class Coriobacteriia were potential biomarker for the antidepressant effects of ketamine in an inflammation model.
30528936	5	66	theme	linear	954:959	arg1	LDA					984:986	LDA	984:986	LDA	984:986	In addition to α-diversity, β-diversity, such as principal coordinates analysis (PCoA), and linear discriminant analysis (LDA) coupled with effect size measurements (LEfSe), showed a differential profile after ketamine treatment.
30528936	5	66	theme	linear	954:959	arg1	analysis					974:981	linear discriminant analysis	954:981	linear discriminant analysis (LDA) coupled with effect size measurements (LEfSe)	954:1033	In addition to α-diversity, β-diversity, such as principal coordinates analysis (PCoA), and linear discriminant analysis (LDA) coupled with effect size measurements (LEfSe), showed a differential profile after ketamine treatment.
30528936	5	67	theme	differential	1045:1056	arg1	profile					1058:1064	a differential profile	1043:1064	a differential profile	1043:1064	In addition to α-diversity, β-diversity, such as principal coordinates analysis (PCoA), and linear discriminant analysis (LDA) coupled with effect size measurements (LEfSe), showed a differential profile after ketamine treatment.
30528936	2	68	theme	gut-brain	351:359	arg1	axis					361:364	the gut-brain axis	347:364	the gut-brain axis	347:364	Accumulating evidence suggests that gut microbiota via the gut-brain axis play a role in the pathogenesis of depression, thereby contributing to the antidepressant actions of certain compounds.
30528936	1	69	theme	antidepressant	226:239	arg1	effects					241:247	rapid-acting and sustained antidepressant effects	199:247	rapid-acting and sustained antidepressant effects	199:247	Ketamine, an N-methyl-d-aspartic acid receptor (NMDAR) antagonist, elicits rapid-acting and sustained antidepressant effects in treatment-resistant depressed patients.
30528936	3	70	theme	depression	634:643	arg1	model					625:629	lipopolysaccharide (LPS)-induced inflammation model	579:629	lipopolysaccharide (LPS)-induced inflammation model of depression	579:643	Here we investigated the role of gut microbiota in the antidepressant effects of ketamine in lipopolysaccharide (LPS)-induced inflammation model of depression.
30528936	7	71	theme	class	1294:1298	arg1	Coriobacteriia					1300:1313	the class Coriobacteriia	1290:1313	the class Coriobacteriia	1290:1313	Interestingly, two bacteria, including the phylum Actinobacteria and the class Coriobacteriia were significantly correlated with the immobility time of FST.
30528936	4	72	theme	forced	724:729	arg1	FST					746:748	FST	746:748	FST	746:748	Ketamine (10 mg/kg) significantly attenuated the increased immobility time in forced swimming test (FST), which was associated with the improvements in α-diversity, consisting of Shannon, Simpson and Chao 1 indices.
30528936	4	72	theme	forced	724:729	arg1	test					740:743	forced swimming test	724:743	forced swimming test (FST)	724:749	Ketamine (10 mg/kg) significantly attenuated the increased immobility time in forced swimming test (FST), which was associated with the improvements in α-diversity, consisting of Shannon, Simpson and Chao 1 indices.
30528936	6	73	theme	treated	1175:1181	arg1	mice					1183:1186	the LPS + saline treated mice	1158:1186	the LPS + saline treated mice	1158:1186	Furthermore, a total of 30 bacteria were significantly altered in the LPS + saline treated mice and LPS + ketamine treated mice.
30528936	1	74	theme	N-methyl-d-aspartic	137:155	arg1	NMDAR					172:176	NMDAR	172:176	NMDAR	172:176	Ketamine, an N-methyl-d-aspartic acid receptor (NMDAR) antagonist, elicits rapid-acting and sustained antidepressant effects in treatment-resistant depressed patients.
30528936	1	74	theme	N-methyl-d-aspartic	137:155	arg1	receptor					162:169	N-methyl-d-aspartic acid receptor	137:169	an N-methyl-d-aspartic acid receptor (NMDAR) antagonist	134:188	Ketamine, an N-methyl-d-aspartic acid receptor (NMDAR) antagonist, elicits rapid-acting and sustained antidepressant effects in treatment-resistant depressed patients.
30528936	3	75	from	effects	556:562	arg1	model					625:629	lipopolysaccharide (LPS)-induced inflammation model	579:629	lipopolysaccharide (LPS)-induced inflammation model of depression	579:643	Here we investigated the role of gut microbiota in the antidepressant effects of ketamine in lipopolysaccharide (LPS)-induced inflammation model of depression.
29935094	10	0	dep	dexamethasone	1657:1669	arg1	18 days					1683:1689	18 days	1683:1689	18 days	1683:1689	This efficacy of soya PC was in extent similar to dexamethasone (2.5 mg/kg, 18 days, i.p.).
29935094	10	0	dep	dexamethasone	1657:1669	arg1	2.5 mg/kg					1672:1680	2.5 mg/kg	1672:1680	2.5 mg/kg	1672:1680	This efficacy of soya PC was in extent similar to dexamethasone (2.5 mg/kg, 18 days, i.p.).
29935094	6	1	theme	lavage	1128:1133	arg1	BALf					1142:1145	BALf	1142:1145	BALf	1142:1145	At the end of the experiment, we performed lung function parameters (respiratory rate, tidal volume, airflow rate), inflammatory cytokines (interleukin [IL]-4, IL-5, IL-13), total and differential leukocytes in blood as well as bronchoalveolar lavage fluid (BALf) and histological examinations.
29935094	6	1	theme	lavage	1128:1133	arg1	fluid					1135:1139	bronchoalveolar lavage fluid	1112:1139	blood as well as bronchoalveolar lavage fluid (BALf)	1095:1146	At the end of the experiment, we performed lung function parameters (respiratory rate, tidal volume, airflow rate), inflammatory cytokines (interleukin [IL]-4, IL-5, IL-13), total and differential leukocytes in blood as well as bronchoalveolar lavage fluid (BALf) and histological examinations.
29935094	7	2	theme	acid	1273:1276	arg1	chain					1296:1300	linoleic acid (18:2) fatty acid chain	1264:1300	linoleic acid (18:2) fatty acid chain	1264:1300	The computational study indicated that TLR4 antagonist activity of soya PC is due to linoleic acid (18:2) fatty acid chain.
29935094	4	3	theme	soya	577:580	arg1	PC					582:583	soya PC	577:583	soya PC	577:583	Based on the computational study, TLR4 antagonist activity of soya PC was confirmed in in vitro lipopolysaccharide (LPS)-induced neutrophil adhesion assay.
29935094	12	4	theme	soya	1793:1796	arg1	PC					1798:1799	soya PC	1793:1799	soya PC	1793:1799	The protective action of soya PC may be due to TLR4 antagonist activity and linoleic acid composition.
29935094	7	5	theme	acid	1291:1294	arg1	chain					1296:1300	linoleic acid (18:2) fatty acid chain	1264:1300	linoleic acid (18:2) fatty acid chain	1264:1300	The computational study indicated that TLR4 antagonist activity of soya PC is due to linoleic acid (18:2) fatty acid chain.
29935094	1	6	theme	immune	166:171	arg1	system					173:178	the innate immune system	155:178	the innate immune system	155:178	Toll-like receptor-4 (TLR4) is a key component of the innate immune system and activation of TLR4 signaling has a significant role in the pathogenesis of asthma.
29935094	9	7	theme	lung	1504:1507	arg1	total					1530:1534	total	1530:1534	total	1530:1534	The treatment of soya PC (5 and 10 mg/kg, 18 days, i.p.) significantly improved the lung function parameters, total and differential leukocyte counts in blood and BALf in asthmatic rats.
29935094	9	7	theme	lung	1504:1507	arg1	parameters					1518:1527	the lung function parameters	1500:1527	the lung function parameters	1500:1527	The treatment of soya PC (5 and 10 mg/kg, 18 days, i.p.) significantly improved the lung function parameters, total and differential leukocyte counts in blood and BALf in asthmatic rats.
29935094	9	7	theme	lung	1504:1507	arg1	counts					1563:1568	differential leukocyte counts	1540:1568	differential leukocyte counts	1540:1568	The treatment of soya PC (5 and 10 mg/kg, 18 days, i.p.) significantly improved the lung function parameters, total and differential leukocyte counts in blood and BALf in asthmatic rats.
29935094	1	8	theme	asthma	259:264	arg1	pathogenesis					243:254	the pathogenesis	239:254	the pathogenesis of asthma	239:264	Toll-like receptor-4 (TLR4) is a key component of the innate immune system and activation of TLR4 signaling has a significant role in the pathogenesis of asthma.
29935094	4	9	theme	neutrophil	644:653	arg1	assay					664:668	in vitro lipopolysaccharide (LPS)-induced neutrophil adhesion assay	602:668	in vitro lipopolysaccharide (LPS)-induced neutrophil adhesion assay	602:668	Based on the computational study, TLR4 antagonist activity of soya PC was confirmed in in vitro lipopolysaccharide (LPS)-induced neutrophil adhesion assay.
29935094	6	10	from	parameters	941:950	arg1	BALf					1142:1145	BALf	1142:1145	BALf	1142:1145	At the end of the experiment, we performed lung function parameters (respiratory rate, tidal volume, airflow rate), inflammatory cytokines (interleukin [IL]-4, IL-5, IL-13), total and differential leukocytes in blood as well as bronchoalveolar lavage fluid (BALf) and histological examinations.
29935094	6	10	from	parameters	941:950	arg1	blood					1095:1099	blood	1095:1099	blood as well as bronchoalveolar lavage fluid (BALf)	1095:1146	At the end of the experiment, we performed lung function parameters (respiratory rate, tidal volume, airflow rate), inflammatory cytokines (interleukin [IL]-4, IL-5, IL-13), total and differential leukocytes in blood as well as bronchoalveolar lavage fluid (BALf) and histological examinations.
29935094	6	10	from	parameters	941:950	arg1	fluid					1135:1139	bronchoalveolar lavage fluid	1112:1139	blood as well as bronchoalveolar lavage fluid (BALf)	1095:1146	At the end of the experiment, we performed lung function parameters (respiratory rate, tidal volume, airflow rate), inflammatory cytokines (interleukin [IL]-4, IL-5, IL-13), total and differential leukocytes in blood as well as bronchoalveolar lavage fluid (BALf) and histological examinations.
29935094	6	11	theme	total	1058:1062	arg1	leukocytes					1081:1090	total and differential leukocytes	1058:1090	total and differential leukocytes in blood as well as bronchoalveolar lavage fluid (BALf)	1058:1146	At the end of the experiment, we performed lung function parameters (respiratory rate, tidal volume, airflow rate), inflammatory cytokines (interleukin [IL]-4, IL-5, IL-13), total and differential leukocytes in blood as well as bronchoalveolar lavage fluid (BALf) and histological examinations.
29935094	7	12	theme	soya	1246:1249	arg1	PC					1251:1252	soya PC	1246:1252	soya PC	1246:1252	The computational study indicated that TLR4 antagonist activity of soya PC is due to linoleic acid (18:2) fatty acid chain.
29935094	5	13	theme	in	678:679	arg1	study					686:690	the in vivo study	674:690	the in vivo study	674:690	In the in vivo study, rats were sensitized with ovalbumin (OVA) (100 μg/kg, i.p.) on the 7th, 14th and 21st days and challenged intranasally with OVA (100 μg/100 μL) and LPS (10 ng/100 μL), 4 days/wk for 3 weeks.
29935094	11	14	from	terms	1749:1753	arg1	superior					1720:1727	superior	1720:1727	superior	1720:1727	However, soya PC was superior to dexamethasone in terms of benefits.
29935094	6	15	theme	histological	1152:1163	arg1	examinations					1165:1176	histological examinations	1152:1176	histological examinations	1152:1176	At the end of the experiment, we performed lung function parameters (respiratory rate, tidal volume, airflow rate), inflammatory cytokines (interleukin [IL]-4, IL-5, IL-13), total and differential leukocytes in blood as well as bronchoalveolar lavage fluid (BALf) and histological examinations.
29935094	10	16	theme	soya	1624:1627	arg1	PC					1629:1630	soya PC	1624:1630	soya PC	1624:1630	This efficacy of soya PC was in extent similar to dexamethasone (2.5 mg/kg, 18 days, i.p.).
29935094	1	17	theme	signaling	203:211	arg1	activation					184:193	activation	184:193	activation of TLR4 signaling	184:211	Toll-like receptor-4 (TLR4) is a key component of the innate immune system and activation of TLR4 signaling has a significant role in the pathogenesis of asthma.
29935094	9	18	theme	differential	1540:1551	arg1	parameters					1518:1527	the lung function parameters	1500:1527	the lung function parameters	1500:1527	The treatment of soya PC (5 and 10 mg/kg, 18 days, i.p.) significantly improved the lung function parameters, total and differential leukocyte counts in blood and BALf in asthmatic rats.
29935094	9	18	theme	differential	1540:1551	arg1	counts					1563:1568	differential leukocyte counts	1540:1568	differential leukocyte counts	1540:1568	The treatment of soya PC (5 and 10 mg/kg, 18 days, i.p.) significantly improved the lung function parameters, total and differential leukocyte counts in blood and BALf in asthmatic rats.
29935094	8	19	theme	concentration-dependent	1377:1399	arg1	manner					1401:1406	a concentration-dependent manner	1375:1406	a concentration-dependent manner	1375:1406	Soya PC significantly suppressed the LPS-induced neutrophil adhesion in a concentration-dependent manner to 1 μg/mL.
29935094	7	20	theme	TLR4	1218:1221	arg1	activity					1234:1241	TLR4 antagonist activity	1218:1241	TLR4 antagonist activity of soya PC	1218:1252	The computational study indicated that TLR4 antagonist activity of soya PC is due to linoleic acid (18:2) fatty acid chain.
29935094	12	21	theme	linoleic	1844:1851	arg1	composition					1858:1868	linoleic acid composition	1844:1868	linoleic acid composition	1844:1868	The protective action of soya PC may be due to TLR4 antagonist activity and linoleic acid composition.
29935094	1	22	contain	has	213:215	arg2	role					231:234	a significant role	217:234	a significant role	217:234	Toll-like receptor-4 (TLR4) is a key component of the innate immune system and activation of TLR4 signaling has a significant role in the pathogenesis of asthma.
29935094	1	22	contain	has	213:215	arg1	activation					184:193	activation	184:193	activation of TLR4 signaling	184:211	Toll-like receptor-4 (TLR4) is a key component of the innate immune system and activation of TLR4 signaling has a significant role in the pathogenesis of asthma.
29935094	11	23	from	superior	1720:1727	arg1	terms					1749:1753	terms	1749:1753	terms of benefits	1749:1765	However, soya PC was superior to dexamethasone in terms of benefits.
29935094	8	24	theme	Soya	1303:1306	arg1	PC					1308:1309	Soya PC	1303:1309	Soya PC	1303:1309	Soya PC significantly suppressed the LPS-induced neutrophil adhesion in a concentration-dependent manner to 1 μg/mL.
29935094	7	25	theme	computational	1183:1195	arg1	study					1197:1201	The computational study	1179:1201	The computational study	1179:1201	The computational study indicated that TLR4 antagonist activity of soya PC is due to linoleic acid (18:2) fatty acid chain.
29935094	12	26	theme	TLR4	1815:1818	arg1	activity					1831:1838	TLR4 antagonist activity	1815:1838	TLR4 antagonist activity	1815:1838	The protective action of soya PC may be due to TLR4 antagonist activity and linoleic acid composition.
29935094	11	27	theme	benefits	1758:1765	arg1	terms					1749:1753	terms	1749:1753	terms of benefits	1749:1765	However, soya PC was superior to dexamethasone in terms of benefits.
29935094	0	28	from	activity	18:25	arg1	rats					99:102	OVA-LPS-induced asthmatic rats	73:102	OVA-LPS-induced asthmatic rats	73:102	An anti-asthmatic activity of natural Toll-like receptor-4 antagonist in OVA-LPS-induced asthmatic rats.
29935094	0	29	theme	asthmatic	89:97	arg1	rats					99:102	OVA-LPS-induced asthmatic rats	73:102	OVA-LPS-induced asthmatic rats	73:102	An anti-asthmatic activity of natural Toll-like receptor-4 antagonist in OVA-LPS-induced asthmatic rats.
29935094	9	30	theme	asthmatic	1591:1599	arg1	rats					1601:1604	asthmatic rats	1591:1604	asthmatic rats	1591:1604	The treatment of soya PC (5 and 10 mg/kg, 18 days, i.p.) significantly improved the lung function parameters, total and differential leukocyte counts in blood and BALf in asthmatic rats.
29935094	8	31	theme	neutrophil	1352:1361	arg1	adhesion					1363:1370	the LPS-induced neutrophil adhesion	1336:1370	the LPS-induced neutrophil adhesion in a concentration-dependent manner to 1 μg/mL	1336:1417	Soya PC significantly suppressed the LPS-induced neutrophil adhesion in a concentration-dependent manner to 1 μg/mL.
29935094	6	32	dep	parameters	941:950	arg1	rate					965:968	respiratory rate	953:968	respiratory rate	953:968	At the end of the experiment, we performed lung function parameters (respiratory rate, tidal volume, airflow rate), inflammatory cytokines (interleukin [IL]-4, IL-5, IL-13), total and differential leukocytes in blood as well as bronchoalveolar lavage fluid (BALf) and histological examinations.
29935094	6	32	dep	parameters	941:950	arg1	volume					977:982	tidal volume	971:982	tidal volume	971:982	At the end of the experiment, we performed lung function parameters (respiratory rate, tidal volume, airflow rate), inflammatory cytokines (interleukin [IL]-4, IL-5, IL-13), total and differential leukocytes in blood as well as bronchoalveolar lavage fluid (BALf) and histological examinations.
29935094	6	32	dep	parameters	941:950	arg1	rate					993:996	airflow rate	985:996	airflow rate	985:996	At the end of the experiment, we performed lung function parameters (respiratory rate, tidal volume, airflow rate), inflammatory cytokines (interleukin [IL]-4, IL-5, IL-13), total and differential leukocytes in blood as well as bronchoalveolar lavage fluid (BALf) and histological examinations.
29935094	2	33	theme	induced	380:386	arg1	asthma					388:393	experimentally induced asthma	365:393	experimentally induced asthma	365:393	Therefore, our objective was to identify the natural TLR4 antagonist and evaluate its activity in experimentally induced asthma.
29935094	3	34	theme	origin	410:415	arg1	antagonist					480:489	a natural TLR4 antagonist	465:489	a natural TLR4 antagonist	465:489	Soya lecithin origin phosphatidylcholine (soya PC) was identified as a natural TLR4 antagonist by computational study.
29935094	3	34	theme	origin	410:415	arg1	phosphatidylcholine					417:435	Soya lecithin origin phosphatidylcholine	396:435	Soya lecithin origin phosphatidylcholine (soya PC)	396:445	Soya lecithin origin phosphatidylcholine (soya PC) was identified as a natural TLR4 antagonist by computational study.
29935094	3	34	theme	origin	410:415	arg1	PC					443:444	soya PC	438:444	soya PC	438:444	Soya lecithin origin phosphatidylcholine (soya PC) was identified as a natural TLR4 antagonist by computational study.
29935094	6	35	from	examinations	1165:1176	arg1	BALf					1142:1145	BALf	1142:1145	BALf	1142:1145	At the end of the experiment, we performed lung function parameters (respiratory rate, tidal volume, airflow rate), inflammatory cytokines (interleukin [IL]-4, IL-5, IL-13), total and differential leukocytes in blood as well as bronchoalveolar lavage fluid (BALf) and histological examinations.
29935094	6	35	from	examinations	1165:1176	arg1	blood					1095:1099	blood	1095:1099	blood as well as bronchoalveolar lavage fluid (BALf)	1095:1146	At the end of the experiment, we performed lung function parameters (respiratory rate, tidal volume, airflow rate), inflammatory cytokines (interleukin [IL]-4, IL-5, IL-13), total and differential leukocytes in blood as well as bronchoalveolar lavage fluid (BALf) and histological examinations.
29935094	6	35	from	examinations	1165:1176	arg1	fluid					1135:1139	bronchoalveolar lavage fluid	1112:1139	blood as well as bronchoalveolar lavage fluid (BALf)	1095:1146	At the end of the experiment, we performed lung function parameters (respiratory rate, tidal volume, airflow rate), inflammatory cytokines (interleukin [IL]-4, IL-5, IL-13), total and differential leukocytes in blood as well as bronchoalveolar lavage fluid (BALf) and histological examinations.
29935094	1	36	theme	Toll-like	105:113	arg1	component					142:150	a key component	136:150	a key component of the innate immune system	136:178	Toll-like receptor-4 (TLR4) is a key component of the innate immune system and activation of TLR4 signaling has a significant role in the pathogenesis of asthma.
29935094	1	36	theme	Toll-like	105:113	arg1	TLR4					127:130	TLR4	127:130	TLR4	127:130	Toll-like receptor-4 (TLR4) is a key component of the innate immune system and activation of TLR4 signaling has a significant role in the pathogenesis of asthma.
29935094	1	36	theme	Toll-like	105:113	arg1	receptor-4					115:124	Toll-like receptor-4	105:124	Toll-like receptor-4 (TLR4)	105:131	Toll-like receptor-4 (TLR4) is a key component of the innate immune system and activation of TLR4 signaling has a significant role in the pathogenesis of asthma.
29935094	4	37	theme	computational	528:540	arg1	study					542:546	the computational study	524:546	the computational study	524:546	Based on the computational study, TLR4 antagonist activity of soya PC was confirmed in in vitro lipopolysaccharide (LPS)-induced neutrophil adhesion assay.
29935094	6	38	from	cytokines	1013:1021	arg1	BALf					1142:1145	BALf	1142:1145	BALf	1142:1145	At the end of the experiment, we performed lung function parameters (respiratory rate, tidal volume, airflow rate), inflammatory cytokines (interleukin [IL]-4, IL-5, IL-13), total and differential leukocytes in blood as well as bronchoalveolar lavage fluid (BALf) and histological examinations.
29935094	6	38	from	cytokines	1013:1021	arg1	blood					1095:1099	blood	1095:1099	blood as well as bronchoalveolar lavage fluid (BALf)	1095:1146	At the end of the experiment, we performed lung function parameters (respiratory rate, tidal volume, airflow rate), inflammatory cytokines (interleukin [IL]-4, IL-5, IL-13), total and differential leukocytes in blood as well as bronchoalveolar lavage fluid (BALf) and histological examinations.
29935094	6	38	from	cytokines	1013:1021	arg1	fluid					1135:1139	bronchoalveolar lavage fluid	1112:1139	blood as well as bronchoalveolar lavage fluid (BALf)	1095:1146	At the end of the experiment, we performed lung function parameters (respiratory rate, tidal volume, airflow rate), inflammatory cytokines (interleukin [IL]-4, IL-5, IL-13), total and differential leukocytes in blood as well as bronchoalveolar lavage fluid (BALf) and histological examinations.
29935094	6	39	theme	function	932:939	arg1	parameters					941:950	lung function parameters	927:950	lung function parameters (respiratory rate, tidal volume, airflow rate)	927:997	At the end of the experiment, we performed lung function parameters (respiratory rate, tidal volume, airflow rate), inflammatory cytokines (interleukin [IL]-4, IL-5, IL-13), total and differential leukocytes in blood as well as bronchoalveolar lavage fluid (BALf) and histological examinations.
29935094	3	40	theme	Soya	396:399	arg1	antagonist					480:489	a natural TLR4 antagonist	465:489	a natural TLR4 antagonist	465:489	Soya lecithin origin phosphatidylcholine (soya PC) was identified as a natural TLR4 antagonist by computational study.
29935094	3	40	theme	Soya	396:399	arg1	phosphatidylcholine					417:435	Soya lecithin origin phosphatidylcholine	396:435	Soya lecithin origin phosphatidylcholine (soya PC)	396:445	Soya lecithin origin phosphatidylcholine (soya PC) was identified as a natural TLR4 antagonist by computational study.
29935094	3	40	theme	Soya	396:399	arg1	PC					443:444	soya PC	438:444	soya PC	438:444	Soya lecithin origin phosphatidylcholine (soya PC) was identified as a natural TLR4 antagonist by computational study.
29935094	11	41	theme	soya	1708:1711	arg1	PC					1713:1714	soya PC	1708:1714	soya PC	1708:1714	However, soya PC was superior to dexamethasone in terms of benefits.
29935094	1	42	theme	innate	159:164	arg1	system					173:178	the innate immune system	155:178	the innate immune system	155:178	Toll-like receptor-4 (TLR4) is a key component of the innate immune system and activation of TLR4 signaling has a significant role in the pathogenesis of asthma.
29935094	0	43	theme	natural	30:36	arg1	receptor-4					48:57	natural Toll-like receptor-4	30:57	natural Toll-like receptor-4 antagonist	30:68	An anti-asthmatic activity of natural Toll-like receptor-4 antagonist in OVA-LPS-induced asthmatic rats.
29935094	9	44	theme	PC	1442:1443	arg1	treatment					1424:1432	The treatment	1420:1432	The treatment of soya PC (5 and 10 mg/kg, 18 days, i.p.)	1420:1475	The treatment of soya PC (5 and 10 mg/kg, 18 days, i.p.) significantly improved the lung function parameters, total and differential leukocyte counts in blood and BALf in asthmatic rats.
29935094	3	45	theme	natural	467:473	arg1	phosphatidylcholine					417:435	Soya lecithin origin phosphatidylcholine	396:435	Soya lecithin origin phosphatidylcholine (soya PC)	396:445	Soya lecithin origin phosphatidylcholine (soya PC) was identified as a natural TLR4 antagonist by computational study.
29935094	3	45	theme	natural	467:473	arg1	antagonist					480:489	a natural TLR4 antagonist	465:489	a natural TLR4 antagonist	465:489	Soya lecithin origin phosphatidylcholine (soya PC) was identified as a natural TLR4 antagonist by computational study.
29935094	0	46	theme	receptor-4	48:57	arg1	antagonist					59:68	natural Toll-like receptor-4 antagonist	30:68	natural Toll-like receptor-4 antagonist	30:68	An anti-asthmatic activity of natural Toll-like receptor-4 antagonist in OVA-LPS-induced asthmatic rats.
29935094	9	47	from	BALf	1583:1586	arg1	rats					1601:1604	asthmatic rats	1591:1604	asthmatic rats	1591:1604	The treatment of soya PC (5 and 10 mg/kg, 18 days, i.p.) significantly improved the lung function parameters, total and differential leukocyte counts in blood and BALf in asthmatic rats.
29935094	4	48	dep	in	602:603	arg1	vitro					605:609	vitro	605:609	vitro	605:609	Based on the computational study, TLR4 antagonist activity of soya PC was confirmed in in vitro lipopolysaccharide (LPS)-induced neutrophil adhesion assay.
29935094	9	49	from	blood	1573:1577	arg1	rats					1601:1604	asthmatic rats	1591:1604	asthmatic rats	1591:1604	The treatment of soya PC (5 and 10 mg/kg, 18 days, i.p.) significantly improved the lung function parameters, total and differential leukocyte counts in blood and BALf in asthmatic rats.
29935094	4	50	theme	PC	582:583	arg1	activity					565:572	TLR4 antagonist activity	549:572	TLR4 antagonist activity of soya PC	549:583	Based on the computational study, TLR4 antagonist activity of soya PC was confirmed in in vitro lipopolysaccharide (LPS)-induced neutrophil adhesion assay.
29935094	3	51	theme	computational	494:506	arg1	study					508:512	computational study	494:512	computational study	494:512	Soya lecithin origin phosphatidylcholine (soya PC) was identified as a natural TLR4 antagonist by computational study.
29935094	1	52	theme	significant	219:229	arg1	role					231:234	a significant role	217:234	a significant role	217:234	Toll-like receptor-4 (TLR4) is a key component of the innate immune system and activation of TLR4 signaling has a significant role in the pathogenesis of asthma.
29935094	8	53	from	adhesion	1363:1370	arg1	manner					1401:1406	a concentration-dependent manner	1375:1406	a concentration-dependent manner	1375:1406	Soya PC significantly suppressed the LPS-induced neutrophil adhesion in a concentration-dependent manner to 1 μg/mL.
29935094	6	54	theme	tidal	971:975	arg1	rate					965:968	respiratory rate	953:968	respiratory rate	953:968	At the end of the experiment, we performed lung function parameters (respiratory rate, tidal volume, airflow rate), inflammatory cytokines (interleukin [IL]-4, IL-5, IL-13), total and differential leukocytes in blood as well as bronchoalveolar lavage fluid (BALf) and histological examinations.
29935094	6	54	theme	tidal	971:975	arg1	volume					977:982	tidal volume	971:982	tidal volume	971:982	At the end of the experiment, we performed lung function parameters (respiratory rate, tidal volume, airflow rate), inflammatory cytokines (interleukin [IL]-4, IL-5, IL-13), total and differential leukocytes in blood as well as bronchoalveolar lavage fluid (BALf) and histological examinations.
29935094	4	55	theme	antagonist	554:563	arg1	activity					565:572	TLR4 antagonist activity	549:572	TLR4 antagonist activity of soya PC	549:583	Based on the computational study, TLR4 antagonist activity of soya PC was confirmed in in vitro lipopolysaccharide (LPS)-induced neutrophil adhesion assay.
29935094	6	56	theme	bronchoalveolar	1112:1126	arg1	BALf					1142:1145	BALf	1142:1145	BALf	1142:1145	At the end of the experiment, we performed lung function parameters (respiratory rate, tidal volume, airflow rate), inflammatory cytokines (interleukin [IL]-4, IL-5, IL-13), total and differential leukocytes in blood as well as bronchoalveolar lavage fluid (BALf) and histological examinations.
29935094	6	56	theme	bronchoalveolar	1112:1126	arg1	fluid					1135:1139	bronchoalveolar lavage fluid	1112:1139	blood as well as bronchoalveolar lavage fluid (BALf)	1095:1146	At the end of the experiment, we performed lung function parameters (respiratory rate, tidal volume, airflow rate), inflammatory cytokines (interleukin [IL]-4, IL-5, IL-13), total and differential leukocytes in blood as well as bronchoalveolar lavage fluid (BALf) and histological examinations.
29935094	12	57	theme	protective	1772:1781	arg1	action					1783:1788	The protective action	1768:1788	The protective action of soya PC	1768:1799	The protective action of soya PC may be due to TLR4 antagonist activity and linoleic acid composition.
29935094	12	57	theme	protective	1772:1781	arg1	due					1808:1810	due	1808:1810	due	1808:1810	The protective action of soya PC may be due to TLR4 antagonist activity and linoleic acid composition.
29935094	7	58	theme	fatty	1285:1289	arg1	chain					1296:1300	linoleic acid (18:2) fatty acid chain	1264:1300	linoleic acid (18:2) fatty acid chain	1264:1300	The computational study indicated that TLR4 antagonist activity of soya PC is due to linoleic acid (18:2) fatty acid chain.
29935094	2	59	theme	TLR4	320:323	arg1	antagonist					325:334	the natural TLR4 antagonist	308:334	the natural TLR4 antagonist	308:334	Therefore, our objective was to identify the natural TLR4 antagonist and evaluate its activity in experimentally induced asthma.
29935094	5	60	theme	21st	774:777	arg1	days					779:782	21st days	774:782	21st days	774:782	In the in vivo study, rats were sensitized with ovalbumin (OVA) (100 μg/kg, i.p.) on the 7th, 14th and 21st days and challenged intranasally with OVA (100 μg/100 μL) and LPS (10 ng/100 μL), 4 days/wk for 3 weeks.
29935094	5	60	theme	21st	774:777	arg1	7th					760:762	7th	760:762	7th	760:762	In the in vivo study, rats were sensitized with ovalbumin (OVA) (100 μg/kg, i.p.) on the 7th, 14th and 21st days and challenged intranasally with OVA (100 μg/100 μL) and LPS (10 ng/100 μL), 4 days/wk for 3 weeks.
29935094	7	61	theme	linoleic	1264:1271	arg1	18:2					1279:1282	18:2	1279:1282	18:2	1279:1282	The computational study indicated that TLR4 antagonist activity of soya PC is due to linoleic acid (18:2) fatty acid chain.
29935094	7	61	theme	linoleic	1264:1271	arg1	acid					1273:1276	linoleic acid	1264:1276	linoleic acid (18:2) fatty acid chain	1264:1300	The computational study indicated that TLR4 antagonist activity of soya PC is due to linoleic acid (18:2) fatty acid chain.
29935094	6	62	dep	cytokines	1013:1021	arg1	[IL					1036:1038	interleukin [IL]-4	1024:1041	interleukin [IL]-4	1024:1041	At the end of the experiment, we performed lung function parameters (respiratory rate, tidal volume, airflow rate), inflammatory cytokines (interleukin [IL]-4, IL-5, IL-13), total and differential leukocytes in blood as well as bronchoalveolar lavage fluid (BALf) and histological examinations.
29935094	6	62	dep	cytokines	1013:1021	arg1	IL-13					1050:1054	IL-13	1050:1054	IL-13	1050:1054	At the end of the experiment, we performed lung function parameters (respiratory rate, tidal volume, airflow rate), inflammatory cytokines (interleukin [IL]-4, IL-5, IL-13), total and differential leukocytes in blood as well as bronchoalveolar lavage fluid (BALf) and histological examinations.
29935094	6	62	dep	cytokines	1013:1021	arg1	IL-5					1044:1047	IL-5	1044:1047	IL-5	1044:1047	At the end of the experiment, we performed lung function parameters (respiratory rate, tidal volume, airflow rate), inflammatory cytokines (interleukin [IL]-4, IL-5, IL-13), total and differential leukocytes in blood as well as bronchoalveolar lavage fluid (BALf) and histological examinations.
29935094	1	63	theme	system	173:178	arg1	component					142:150	a key component	136:150	a key component of the innate immune system	136:178	Toll-like receptor-4 (TLR4) is a key component of the innate immune system and activation of TLR4 signaling has a significant role in the pathogenesis of asthma.
29935094	1	63	theme	system	173:178	arg1	receptor-4					115:124	Toll-like receptor-4	105:124	Toll-like receptor-4 (TLR4)	105:131	Toll-like receptor-4 (TLR4) is a key component of the innate immune system and activation of TLR4 signaling has a significant role in the pathogenesis of asthma.
29935094	9	64	theme	function	1509:1516	arg1	total					1530:1534	total	1530:1534	total	1530:1534	The treatment of soya PC (5 and 10 mg/kg, 18 days, i.p.) significantly improved the lung function parameters, total and differential leukocyte counts in blood and BALf in asthmatic rats.
29935094	9	64	theme	function	1509:1516	arg1	parameters					1518:1527	the lung function parameters	1500:1527	the lung function parameters	1500:1527	The treatment of soya PC (5 and 10 mg/kg, 18 days, i.p.) significantly improved the lung function parameters, total and differential leukocyte counts in blood and BALf in asthmatic rats.
29935094	9	64	theme	function	1509:1516	arg1	counts					1563:1568	differential leukocyte counts	1540:1568	differential leukocyte counts	1540:1568	The treatment of soya PC (5 and 10 mg/kg, 18 days, i.p.) significantly improved the lung function parameters, total and differential leukocyte counts in blood and BALf in asthmatic rats.
29935094	6	65	theme	interleukin	1024:1034	arg1	[IL					1036:1038	interleukin [IL]-4	1024:1041	interleukin [IL]-4	1024:1041	At the end of the experiment, we performed lung function parameters (respiratory rate, tidal volume, airflow rate), inflammatory cytokines (interleukin [IL]-4, IL-5, IL-13), total and differential leukocytes in blood as well as bronchoalveolar lavage fluid (BALf) and histological examinations.
29935094	6	65	theme	interleukin	1024:1034	arg1	IL-13					1050:1054	IL-13	1050:1054	IL-13	1050:1054	At the end of the experiment, we performed lung function parameters (respiratory rate, tidal volume, airflow rate), inflammatory cytokines (interleukin [IL]-4, IL-5, IL-13), total and differential leukocytes in blood as well as bronchoalveolar lavage fluid (BALf) and histological examinations.
29935094	6	65	theme	interleukin	1024:1034	arg1	IL-5					1044:1047	IL-5	1044:1047	IL-5	1044:1047	At the end of the experiment, we performed lung function parameters (respiratory rate, tidal volume, airflow rate), inflammatory cytokines (interleukin [IL]-4, IL-5, IL-13), total and differential leukocytes in blood as well as bronchoalveolar lavage fluid (BALf) and histological examinations.
29935094	4	66	theme	-induced	635:642	arg1	assay					664:668	in vitro lipopolysaccharide (LPS)-induced neutrophil adhesion assay	602:668	in vitro lipopolysaccharide (LPS)-induced neutrophil adhesion assay	602:668	Based on the computational study, TLR4 antagonist activity of soya PC was confirmed in in vitro lipopolysaccharide (LPS)-induced neutrophil adhesion assay.
29935094	7	67	theme	PC	1251:1252	arg1	activity					1234:1241	TLR4 antagonist activity	1218:1241	TLR4 antagonist activity of soya PC	1218:1252	The computational study indicated that TLR4 antagonist activity of soya PC is due to linoleic acid (18:2) fatty acid chain.
29935094	4	68	theme	in	602:603	arg1	assay					664:668	in vitro lipopolysaccharide (LPS)-induced neutrophil adhesion assay	602:668	in vitro lipopolysaccharide (LPS)-induced neutrophil adhesion assay	602:668	Based on the computational study, TLR4 antagonist activity of soya PC was confirmed in in vitro lipopolysaccharide (LPS)-induced neutrophil adhesion assay.
29935094	2	69	theme	natural	312:318	arg1	antagonist					325:334	the natural TLR4 antagonist	308:334	the natural TLR4 antagonist	308:334	Therefore, our objective was to identify the natural TLR4 antagonist and evaluate its activity in experimentally induced asthma.
29935094	10	70	theme	PC	1629:1630	arg1	efficacy					1612:1619	This efficacy	1607:1619	This efficacy of soya PC	1607:1630	This efficacy of soya PC was in extent similar to dexamethasone (2.5 mg/kg, 18 days, i.p.).
29935094	9	71	dep	PC	1442:1443	arg1	10 mg/kg					1452:1459	10 mg/kg	1452:1459	10 mg/kg	1452:1459	The treatment of soya PC (5 and 10 mg/kg, 18 days, i.p.) significantly improved the lung function parameters, total and differential leukocyte counts in blood and BALf in asthmatic rats.
29935094	9	71	dep	PC	1442:1443	arg1	5					1446:1446	5	1446:1446	5	1446:1446	The treatment of soya PC (5 and 10 mg/kg, 18 days, i.p.) significantly improved the lung function parameters, total and differential leukocyte counts in blood and BALf in asthmatic rats.
29935094	9	71	dep	PC	1442:1443	arg1	18 days					1462:1468	18 days	1462:1468	18 days	1462:1468	The treatment of soya PC (5 and 10 mg/kg, 18 days, i.p.) significantly improved the lung function parameters, total and differential leukocyte counts in blood and BALf in asthmatic rats.
29935094	1	72	theme	TLR4	198:201	arg1	signaling					203:211	TLR4 signaling	198:211	TLR4 signaling	198:211	Toll-like receptor-4 (TLR4) is a key component of the innate immune system and activation of TLR4 signaling has a significant role in the pathogenesis of asthma.
29935094	5	73	dep	ovalbumin	719:727	arg1	100 μg/kg					736:744	100 μg/kg	736:744	100 μg/kg	736:744	In the in vivo study, rats were sensitized with ovalbumin (OVA) (100 μg/kg, i.p.) on the 7th, 14th and 21st days and challenged intranasally with OVA (100 μg/100 μL) and LPS (10 ng/100 μL), 4 days/wk for 3 weeks.
29935094	6	74	theme	differential	1068:1079	arg1	leukocytes					1081:1090	total and differential leukocytes	1058:1090	total and differential leukocytes in blood as well as bronchoalveolar lavage fluid (BALf)	1058:1146	At the end of the experiment, we performed lung function parameters (respiratory rate, tidal volume, airflow rate), inflammatory cytokines (interleukin [IL]-4, IL-5, IL-13), total and differential leukocytes in blood as well as bronchoalveolar lavage fluid (BALf) and histological examinations.
29935094	9	75	theme	leukocyte	1553:1561	arg1	parameters					1518:1527	the lung function parameters	1500:1527	the lung function parameters	1500:1527	The treatment of soya PC (5 and 10 mg/kg, 18 days, i.p.) significantly improved the lung function parameters, total and differential leukocyte counts in blood and BALf in asthmatic rats.
29935094	9	75	theme	leukocyte	1553:1561	arg1	counts					1563:1568	differential leukocyte counts	1540:1568	differential leukocyte counts	1540:1568	The treatment of soya PC (5 and 10 mg/kg, 18 days, i.p.) significantly improved the lung function parameters, total and differential leukocyte counts in blood and BALf in asthmatic rats.
29935094	7	76	theme	antagonist	1223:1232	arg1	activity					1234:1241	TLR4 antagonist activity	1218:1241	TLR4 antagonist activity of soya PC	1218:1252	The computational study indicated that TLR4 antagonist activity of soya PC is due to linoleic acid (18:2) fatty acid chain.
29935094	10	77	theme	similar	1646:1652	arg1	extent					1639:1644	extent	1639:1644	extent similar to dexamethasone (2.5 mg/kg, 18 days, i.p.)	1639:1696	This efficacy of soya PC was in extent similar to dexamethasone (2.5 mg/kg, 18 days, i.p.).
29935094	12	78	theme	acid	1853:1856	arg1	composition					1858:1868	linoleic acid composition	1844:1868	linoleic acid composition	1844:1868	The protective action of soya PC may be due to TLR4 antagonist activity and linoleic acid composition.
29935094	6	79	from	leukocytes	1081:1090	arg1	BALf					1142:1145	BALf	1142:1145	BALf	1142:1145	At the end of the experiment, we performed lung function parameters (respiratory rate, tidal volume, airflow rate), inflammatory cytokines (interleukin [IL]-4, IL-5, IL-13), total and differential leukocytes in blood as well as bronchoalveolar lavage fluid (BALf) and histological examinations.
29935094	6	79	from	leukocytes	1081:1090	arg1	blood					1095:1099	blood	1095:1099	blood as well as bronchoalveolar lavage fluid (BALf)	1095:1146	At the end of the experiment, we performed lung function parameters (respiratory rate, tidal volume, airflow rate), inflammatory cytokines (interleukin [IL]-4, IL-5, IL-13), total and differential leukocytes in blood as well as bronchoalveolar lavage fluid (BALf) and histological examinations.
29935094	6	79	from	leukocytes	1081:1090	arg1	fluid					1135:1139	bronchoalveolar lavage fluid	1112:1139	blood as well as bronchoalveolar lavage fluid (BALf)	1095:1146	At the end of the experiment, we performed lung function parameters (respiratory rate, tidal volume, airflow rate), inflammatory cytokines (interleukin [IL]-4, IL-5, IL-13), total and differential leukocytes in blood as well as bronchoalveolar lavage fluid (BALf) and histological examinations.
29935094	0	80	theme	OVA-LPS-induced	73:87	arg1	rats					99:102	OVA-LPS-induced asthmatic rats	73:102	OVA-LPS-induced asthmatic rats	73:102	An anti-asthmatic activity of natural Toll-like receptor-4 antagonist in OVA-LPS-induced asthmatic rats.
29935094	1	81	theme	key	138:140	arg1	component					142:150	a key component	136:150	a key component of the innate immune system	136:178	Toll-like receptor-4 (TLR4) is a key component of the innate immune system and activation of TLR4 signaling has a significant role in the pathogenesis of asthma.
29935094	1	81	theme	key	138:140	arg1	receptor-4					115:124	Toll-like receptor-4	105:124	Toll-like receptor-4 (TLR4)	105:131	Toll-like receptor-4 (TLR4) is a key component of the innate immune system and activation of TLR4 signaling has a significant role in the pathogenesis of asthma.
29935094	6	82	theme	experiment	902:911	arg1	end					891:893	the end	887:893	the end of the experiment	887:911	At the end of the experiment, we performed lung function parameters (respiratory rate, tidal volume, airflow rate), inflammatory cytokines (interleukin [IL]-4, IL-5, IL-13), total and differential leukocytes in blood as well as bronchoalveolar lavage fluid (BALf) and histological examinations.
29935094	4	83	theme	adhesion	655:662	arg1	assay					664:668	in vitro lipopolysaccharide (LPS)-induced neutrophil adhesion assay	602:668	in vitro lipopolysaccharide (LPS)-induced neutrophil adhesion assay	602:668	Based on the computational study, TLR4 antagonist activity of soya PC was confirmed in in vitro lipopolysaccharide (LPS)-induced neutrophil adhesion assay.
29935094	5	84	dep	in	678:679	arg1	vivo					681:684	vivo	681:684	vivo	681:684	In the in vivo study, rats were sensitized with ovalbumin (OVA) (100 μg/kg, i.p.) on the 7th, 14th and 21st days and challenged intranasally with OVA (100 μg/100 μL) and LPS (10 ng/100 μL), 4 days/wk for 3 weeks.
29935094	8	85	theme	LPS-induced	1340:1350	arg1	adhesion					1363:1370	the LPS-induced neutrophil adhesion	1336:1370	the LPS-induced neutrophil adhesion in a concentration-dependent manner to 1 μg/mL	1336:1417	Soya PC significantly suppressed the LPS-induced neutrophil adhesion in a concentration-dependent manner to 1 μg/mL.
29935094	12	86	theme	antagonist	1820:1829	arg1	activity					1831:1838	TLR4 antagonist activity	1815:1838	TLR4 antagonist activity	1815:1838	The protective action of soya PC may be due to TLR4 antagonist activity and linoleic acid composition.
29935094	3	87	theme	soya	438:441	arg1	phosphatidylcholine					417:435	Soya lecithin origin phosphatidylcholine	396:435	Soya lecithin origin phosphatidylcholine (soya PC)	396:445	Soya lecithin origin phosphatidylcholine (soya PC) was identified as a natural TLR4 antagonist by computational study.
29935094	3	87	theme	soya	438:441	arg1	PC					443:444	soya PC	438:444	soya PC	438:444	Soya lecithin origin phosphatidylcholine (soya PC) was identified as a natural TLR4 antagonist by computational study.
29935094	0	88	theme	anti-asthmatic	3:16	arg1	activity					18:25	An anti-asthmatic activity	0:25	An anti-asthmatic activity of natural Toll-like receptor-4 antagonist in OVA-LPS-induced asthmatic rats	0:102	An anti-asthmatic activity of natural Toll-like receptor-4 antagonist in OVA-LPS-induced asthmatic rats.
29935094	6	89	theme	respiratory	953:963	arg1	rate					965:968	respiratory rate	953:968	respiratory rate	953:968	At the end of the experiment, we performed lung function parameters (respiratory rate, tidal volume, airflow rate), inflammatory cytokines (interleukin [IL]-4, IL-5, IL-13), total and differential leukocytes in blood as well as bronchoalveolar lavage fluid (BALf) and histological examinations.
29935094	6	89	theme	respiratory	953:963	arg1	volume					977:982	tidal volume	971:982	tidal volume	971:982	At the end of the experiment, we performed lung function parameters (respiratory rate, tidal volume, airflow rate), inflammatory cytokines (interleukin [IL]-4, IL-5, IL-13), total and differential leukocytes in blood as well as bronchoalveolar lavage fluid (BALf) and histological examinations.
29935094	6	89	theme	respiratory	953:963	arg1	rate					993:996	airflow rate	985:996	airflow rate	985:996	At the end of the experiment, we performed lung function parameters (respiratory rate, tidal volume, airflow rate), inflammatory cytokines (interleukin [IL]-4, IL-5, IL-13), total and differential leukocytes in blood as well as bronchoalveolar lavage fluid (BALf) and histological examinations.
29935094	4	90	theme	TLR4	549:552	arg1	activity					565:572	TLR4 antagonist activity	549:572	TLR4 antagonist activity of soya PC	549:583	Based on the computational study, TLR4 antagonist activity of soya PC was confirmed in in vitro lipopolysaccharide (LPS)-induced neutrophil adhesion assay.
29935094	0	91	theme	Toll-like	38:46	arg1	receptor-4					48:57	natural Toll-like receptor-4	30:57	natural Toll-like receptor-4 antagonist	30:68	An anti-asthmatic activity of natural Toll-like receptor-4 antagonist in OVA-LPS-induced asthmatic rats.
29935094	9	92	theme	soya	1437:1440	arg1	PC					1442:1443	soya PC	1437:1443	soya PC (5 and 10 mg/kg, 18 days, i.p.)	1437:1475	The treatment of soya PC (5 and 10 mg/kg, 18 days, i.p.) significantly improved the lung function parameters, total and differential leukocyte counts in blood and BALf in asthmatic rats.
29935094	6	93	theme	lung	927:930	arg1	parameters					941:950	lung function parameters	927:950	lung function parameters (respiratory rate, tidal volume, airflow rate)	927:997	At the end of the experiment, we performed lung function parameters (respiratory rate, tidal volume, airflow rate), inflammatory cytokines (interleukin [IL]-4, IL-5, IL-13), total and differential leukocytes in blood as well as bronchoalveolar lavage fluid (BALf) and histological examinations.
29935094	3	94	theme	lecithin	401:408	arg1	antagonist					480:489	a natural TLR4 antagonist	465:489	a natural TLR4 antagonist	465:489	Soya lecithin origin phosphatidylcholine (soya PC) was identified as a natural TLR4 antagonist by computational study.
29935094	3	94	theme	lecithin	401:408	arg1	phosphatidylcholine					417:435	Soya lecithin origin phosphatidylcholine	396:435	Soya lecithin origin phosphatidylcholine (soya PC)	396:445	Soya lecithin origin phosphatidylcholine (soya PC) was identified as a natural TLR4 antagonist by computational study.
29935094	3	94	theme	lecithin	401:408	arg1	PC					443:444	soya PC	438:444	soya PC	438:444	Soya lecithin origin phosphatidylcholine (soya PC) was identified as a natural TLR4 antagonist by computational study.
29935094	0	95	theme	antagonist	59:68	arg1	activity					18:25	An anti-asthmatic activity	0:25	An anti-asthmatic activity of natural Toll-like receptor-4 antagonist in OVA-LPS-induced asthmatic rats	0:102	An anti-asthmatic activity of natural Toll-like receptor-4 antagonist in OVA-LPS-induced asthmatic rats.
29935094	12	96	theme	PC	1798:1799	arg1	action					1783:1788	The protective action	1768:1788	The protective action of soya PC	1768:1799	The protective action of soya PC may be due to TLR4 antagonist activity and linoleic acid composition.
29935094	12	96	theme	PC	1798:1799	arg1	due					1808:1810	due	1808:1810	due	1808:1810	The protective action of soya PC may be due to TLR4 antagonist activity and linoleic acid composition.
29935094	2	97	from	activity	353:360	arg1	asthma					388:393	experimentally induced asthma	365:393	experimentally induced asthma	365:393	Therefore, our objective was to identify the natural TLR4 antagonist and evaluate its activity in experimentally induced asthma.
29935094	6	98	theme	inflammatory	1000:1011	arg1	cytokines					1013:1021	inflammatory cytokines	1000:1021	inflammatory cytokines (interleukin [IL]-4, IL-5, IL-13)	1000:1055	At the end of the experiment, we performed lung function parameters (respiratory rate, tidal volume, airflow rate), inflammatory cytokines (interleukin [IL]-4, IL-5, IL-13), total and differential leukocytes in blood as well as bronchoalveolar lavage fluid (BALf) and histological examinations.
29935094	3	99	theme	TLR4	475:478	arg1	phosphatidylcholine					417:435	Soya lecithin origin phosphatidylcholine	396:435	Soya lecithin origin phosphatidylcholine (soya PC)	396:445	Soya lecithin origin phosphatidylcholine (soya PC) was identified as a natural TLR4 antagonist by computational study.
29935094	3	99	theme	TLR4	475:478	arg1	antagonist					480:489	a natural TLR4 antagonist	465:489	a natural TLR4 antagonist	465:489	Soya lecithin origin phosphatidylcholine (soya PC) was identified as a natural TLR4 antagonist by computational study.
29935094	6	100	theme	airflow	985:991	arg1	rate					965:968	respiratory rate	953:968	respiratory rate	953:968	At the end of the experiment, we performed lung function parameters (respiratory rate, tidal volume, airflow rate), inflammatory cytokines (interleukin [IL]-4, IL-5, IL-13), total and differential leukocytes in blood as well as bronchoalveolar lavage fluid (BALf) and histological examinations.
29935094	6	100	theme	airflow	985:991	arg1	rate					993:996	airflow rate	985:996	airflow rate	985:996	At the end of the experiment, we performed lung function parameters (respiratory rate, tidal volume, airflow rate), inflammatory cytokines (interleukin [IL]-4, IL-5, IL-13), total and differential leukocytes in blood as well as bronchoalveolar lavage fluid (BALf) and histological examinations.
30870435	3	0	theme	immunoconfocal	631:644	arg1	morphometry					646:656	immunoconfocal morphometry	631:656	immunoconfocal morphometry	631:656	To explore whether OPCs may actually be recruited within the neurovascular unit (NVU), de facto intervening in its cellular and molecular composition, we quantified by immunoconfocal morphometry the presence of OPCs in contact with brain microvessels, during postnatal cerebral cortex vascularization at postnatal day 6, in wild-type (WT) and NG2 knock-out (NG2KO) mice, and in the cortex of adult naïve and EAE-affected WT and NG2KO mice.
30870435	3	1	attach	presence	662:669	arg1	cortex					845:850	the cortex	841:850	the cortex of adult naïve and EAE-affected WT and NG2KO mice	841:900	To explore whether OPCs may actually be recruited within the neurovascular unit (NVU), de facto intervening in its cellular and molecular composition, we quantified by immunoconfocal morphometry the presence of OPCs in contact with brain microvessels, during postnatal cerebral cortex vascularization at postnatal day 6, in wild-type (WT) and NG2 knock-out (NG2KO) mice, and in the cortex of adult naïve and EAE-affected WT and NG2KO mice.
30870435	3	1	attach	presence	662:669	arg1	WT					798:799	WT	798:799	WT	798:799	To explore whether OPCs may actually be recruited within the neurovascular unit (NVU), de facto intervening in its cellular and molecular composition, we quantified by immunoconfocal morphometry the presence of OPCs in contact with brain microvessels, during postnatal cerebral cortex vascularization at postnatal day 6, in wild-type (WT) and NG2 knock-out (NG2KO) mice, and in the cortex of adult naïve and EAE-affected WT and NG2KO mice.
30870435	3	1	attach	presence	662:669	arg1	contact					682:688	contact	682:688	contact with brain microvessels	682:712	To explore whether OPCs may actually be recruited within the neurovascular unit (NVU), de facto intervening in its cellular and molecular composition, we quantified by immunoconfocal morphometry the presence of OPCs in contact with brain microvessels, during postnatal cerebral cortex vascularization at postnatal day 6, in wild-type (WT) and NG2 knock-out (NG2KO) mice, and in the cortex of adult naïve and EAE-affected WT and NG2KO mice.
30870435	3	1	attach	presence	662:669	arg2	OPCs					674:677	OPCs	674:677	OPCs	674:677	To explore whether OPCs may actually be recruited within the neurovascular unit (NVU), de facto intervening in its cellular and molecular composition, we quantified by immunoconfocal morphometry the presence of OPCs in contact with brain microvessels, during postnatal cerebral cortex vascularization at postnatal day 6, in wild-type (WT) and NG2 knock-out (NG2KO) mice, and in the cortex of adult naïve and EAE-affected WT and NG2KO mice.
30870435	3	1	attach	presence	662:669	arg1	NG2					806:808	NG2	806:808	NG2	806:808	To explore whether OPCs may actually be recruited within the neurovascular unit (NVU), de facto intervening in its cellular and molecular composition, we quantified by immunoconfocal morphometry the presence of OPCs in contact with brain microvessels, during postnatal cerebral cortex vascularization at postnatal day 6, in wild-type (WT) and NG2 knock-out (NG2KO) mice, and in the cortex of adult naïve and EAE-affected WT and NG2KO mice.
30870435	3	1	attach	presence	662:669	arg1	wild-type					787:795	wild-type	787:795	wild-type	787:795	To explore whether OPCs may actually be recruited within the neurovascular unit (NVU), de facto intervening in its cellular and molecular composition, we quantified by immunoconfocal morphometry the presence of OPCs in contact with brain microvessels, during postnatal cerebral cortex vascularization at postnatal day 6, in wild-type (WT) and NG2 knock-out (NG2KO) mice, and in the cortex of adult naïve and EAE-affected WT and NG2KO mice.
30870435	9	2	theme	Tgfb	2012:2015	arg1	expression					2017:2026	Tgfb expression	2012:2026	Tgfb expression	2012:2026	These results were confirmed by real time-PCR that showed Fgf2, Pdgfa and Tgfb expression on isolated cerebral cortex microvessels and by dual RNAscope-immunohistochemistry/in situ hybridization (IHC/ISH), which detected Vegfa and Vegfr2 transcripts on cerebral cortex sections.
30870435	9	3	theme	cortex	2200:2205	arg1	sections					2207:2214	cerebral cortex sections	2191:2214	cerebral cortex sections	2191:2214	These results were confirmed by real time-PCR that showed Fgf2, Pdgfa and Tgfb expression on isolated cerebral cortex microvessels and by dual RNAscope-immunohistochemistry/in situ hybridization (IHC/ISH), which detected Vegfa and Vegfr2 transcripts on cerebral cortex sections.
30870435	9	4	theme	RNAscope-immunohistochemistry/in	2081:2112	arg1	IHC/ISH					2134:2140	IHC/ISH	2134:2140	IHC/ISH	2134:2140	These results were confirmed by real time-PCR that showed Fgf2, Pdgfa and Tgfb expression on isolated cerebral cortex microvessels and by dual RNAscope-immunohistochemistry/in situ hybridization (IHC/ISH), which detected Vegfa and Vegfr2 transcripts on cerebral cortex sections.
30870435	9	4	theme	RNAscope-immunohistochemistry/in	2081:2112	arg1	hybridization					2119:2131	dual RNAscope-immunohistochemistry/in situ hybridization	2076:2131	dual RNAscope-immunohistochemistry/in situ hybridization (IHC/ISH)	2076:2141	These results were confirmed by real time-PCR that showed Fgf2, Pdgfa and Tgfb expression on isolated cerebral cortex microvessels and by dual RNAscope-immunohistochemistry/in situ hybridization (IHC/ISH), which detected Vegfa and Vegfr2 transcripts on cerebral cortex sections.
30870435	8	5	theme	endothelial	1708:1718	arg1	factor					1727:1732	vascular endothelial growth factor	1699:1732	vascular endothelial growth factor A (VEGF-A)	1699:1743	In addition, analysis of the major ligand/receptor systems known to promote OPC proliferation and migration indicated that vascular endothelial growth factor A (VEGF-A), platelet-derived growth factor-AA (PDGF-AA), and the transforming growth factor-β (TGF-β) were the molecules most likely involved in proliferation and recruitment of vascular OPCs during EAE.
30870435	10	6	theme	vascular	2251:2258	arg1	OPCs					2260:2263	vascular OPCs	2251:2263	vascular OPCs	2251:2263	Overall, this study suggests that vascular OPCs, in virtue of their developmental arrangement and response to neuroinflammation and growth factors, could be integrated among the classical NVU cell components.
30870435	6	7	theme	BBB	1406:1408	arg1	integrity					1410:1418	better preserved TJs and BBB integrity	1381:1418	better preserved TJs and BBB integrity	1381:1418	In contrast, EAE-affected NG2KO mice, which did not show any significant increase in vessel-associated OPCs, seemed to retain better preserved TJs and BBB integrity.
30870435	1	8	theme	null	160:163	arg1	mice					165:168	wild type and NG2 null mice	142:168	wild type and NG2 null mice	142:168	A biofunctional analysis of the neurovascular unit in wild type and NG2 null mice.
30870435	11	9	theme	key	2559:2561	arg1	regulator					2563:2571	a key regulator	2557:2571	a key regulator of vascular interactions	2557:2596	Moreover, the synchronized activation of vascular OPCs and pericytes during both BBB development and dysfunction, points to NG2 as a key regulator of vascular interactions.
30870435	4	10	theme	WT	1033:1034	arg1	mice					1036:1039	adult WT mice	1027:1039	adult WT mice	1027:1039	As observed in WT mice during postnatal development, a higher number of juxtavascular and perivascular OPCs was revealed in adult WT mice during EAE compared to adult naïve WT mice.
30870435	10	11	theme	developmental	2285:2297	arg1	arrangement					2299:2309	their developmental arrangement	2279:2309	their developmental arrangement	2279:2309	Overall, this study suggests that vascular OPCs, in virtue of their developmental arrangement and response to neuroinflammation and growth factors, could be integrated among the classical NVU cell components.
30870435	9	12	theme	real	1970:1973	arg1	time-PCR					1975:1982	real time-PCR	1970:1982	real time-PCR that showed Fgf2, Pdgfa and Tgfb expression on isolated cerebral cortex microvessels	1970:2067	These results were confirmed by real time-PCR that showed Fgf2, Pdgfa and Tgfb expression on isolated cerebral cortex microvessels and by dual RNAscope-immunohistochemistry/in situ hybridization (IHC/ISH), which detected Vegfa and Vegfr2 transcripts on cerebral cortex sections.
30870435	8	13	theme	factor	1727:1732	arg1	VEGF-A					1737:1742	VEGF-A	1737:1742	VEGF-A	1737:1742	In addition, analysis of the major ligand/receptor systems known to promote OPC proliferation and migration indicated that vascular endothelial growth factor A (VEGF-A), platelet-derived growth factor-AA (PDGF-AA), and the transforming growth factor-β (TGF-β) were the molecules most likely involved in proliferation and recruitment of vascular OPCs during EAE.
30870435	8	13	theme	factor	1727:1732	arg1	A					1734:1734	vascular endothelial growth factor A	1699:1734	vascular endothelial growth factor A (VEGF-A)	1699:1743	In addition, analysis of the major ligand/receptor systems known to promote OPC proliferation and migration indicated that vascular endothelial growth factor A (VEGF-A), platelet-derived growth factor-AA (PDGF-AA), and the transforming growth factor-β (TGF-β) were the molecules most likely involved in proliferation and recruitment of vascular OPCs during EAE.
30870435	2	14	theme	BBB	295:297	arg1	disruption					300:309	blood-brain barrier (BBB) disruption	274:309	blood-brain barrier (BBB) disruption	274:309	During experimental autoimmune encephalomyelitis (EAE), a model for multiple sclerosis associated with blood-brain barrier (BBB) disruption, oligodendrocyte precursor cells (OPCs) overexpress proteoglycan nerve/glial antigen 2 (NG2), proliferate, and make contacts with the microvessel wall.
30870435	6	15	theme	TJs	1398:1400	arg1	integrity					1410:1418	better preserved TJs and BBB integrity	1381:1418	better preserved TJs and BBB integrity	1381:1418	In contrast, EAE-affected NG2KO mice, which did not show any significant increase in vessel-associated OPCs, seemed to retain better preserved TJs and BBB integrity.
30870435	4	16	theme	perivascular	993:1004	arg1	OPCs					1006:1009	juxtavascular and perivascular OPCs	975:1009	juxtavascular and perivascular OPCs	975:1009	As observed in WT mice during postnatal development, a higher number of juxtavascular and perivascular OPCs was revealed in adult WT mice during EAE compared to adult naïve WT mice.
30870435	11	17	theme	vascular	2576:2583	arg1	interactions					2585:2596	vascular interactions	2576:2596	vascular interactions	2576:2596	Moreover, the synchronized activation of vascular OPCs and pericytes during both BBB development and dysfunction, points to NG2 as a key regulator of vascular interactions.
30870435	3	18	theme	neurovascular	524:536	arg1	NVU					544:546	NVU	544:546	NVU	544:546	To explore whether OPCs may actually be recruited within the neurovascular unit (NVU), de facto intervening in its cellular and molecular composition, we quantified by immunoconfocal morphometry the presence of OPCs in contact with brain microvessels, during postnatal cerebral cortex vascularization at postnatal day 6, in wild-type (WT) and NG2 knock-out (NG2KO) mice, and in the cortex of adult naïve and EAE-affected WT and NG2KO mice.
30870435	3	18	theme	neurovascular	524:536	arg1	unit					538:541	the neurovascular unit	520:541	the neurovascular unit (NVU)	520:547	To explore whether OPCs may actually be recruited within the neurovascular unit (NVU), de facto intervening in its cellular and molecular composition, we quantified by immunoconfocal morphometry the presence of OPCs in contact with brain microvessels, during postnatal cerebral cortex vascularization at postnatal day 6, in wild-type (WT) and NG2 knock-out (NG2KO) mice, and in the cortex of adult naïve and EAE-affected WT and NG2KO mice.
30870435	3	19	theme	cerebral	732:739	arg1	vascularization					748:762	postnatal cerebral cortex vascularization	722:762	postnatal cerebral cortex vascularization at postnatal day 6	722:781	To explore whether OPCs may actually be recruited within the neurovascular unit (NVU), de facto intervening in its cellular and molecular composition, we quantified by immunoconfocal morphometry the presence of OPCs in contact with brain microvessels, during postnatal cerebral cortex vascularization at postnatal day 6, in wild-type (WT) and NG2 knock-out (NG2KO) mice, and in the cortex of adult naïve and EAE-affected WT and NG2KO mice.
30870435	3	20	theme	de	550:551	arg1	facto					553:557	de facto	550:557	de facto	550:557	To explore whether OPCs may actually be recruited within the neurovascular unit (NVU), de facto intervening in its cellular and molecular composition, we quantified by immunoconfocal morphometry the presence of OPCs in contact with brain microvessels, during postnatal cerebral cortex vascularization at postnatal day 6, in wild-type (WT) and NG2 knock-out (NG2KO) mice, and in the cortex of adult naïve and EAE-affected WT and NG2KO mice.
30870435	4	21	theme	adult	1064:1068	arg1	mice					1079:1082	adult naïve WT mice	1064:1082	adult naïve WT mice	1064:1082	As observed in WT mice during postnatal development, a higher number of juxtavascular and perivascular OPCs was revealed in adult WT mice during EAE compared to adult naïve WT mice.
30870435	3	22	from	presence	662:669	arg1	wild-type					787:795	wild-type	787:795	wild-type	787:795	To explore whether OPCs may actually be recruited within the neurovascular unit (NVU), de facto intervening in its cellular and molecular composition, we quantified by immunoconfocal morphometry the presence of OPCs in contact with brain microvessels, during postnatal cerebral cortex vascularization at postnatal day 6, in wild-type (WT) and NG2 knock-out (NG2KO) mice, and in the cortex of adult naïve and EAE-affected WT and NG2KO mice.
30870435	3	22	from	presence	662:669	arg1	cortex					845:850	the cortex	841:850	the cortex of adult naïve and EAE-affected WT and NG2KO mice	841:900	To explore whether OPCs may actually be recruited within the neurovascular unit (NVU), de facto intervening in its cellular and molecular composition, we quantified by immunoconfocal morphometry the presence of OPCs in contact with brain microvessels, during postnatal cerebral cortex vascularization at postnatal day 6, in wild-type (WT) and NG2 knock-out (NG2KO) mice, and in the cortex of adult naïve and EAE-affected WT and NG2KO mice.
30870435	3	22	from	presence	662:669	arg1	WT					798:799	WT	798:799	WT	798:799	To explore whether OPCs may actually be recruited within the neurovascular unit (NVU), de facto intervening in its cellular and molecular composition, we quantified by immunoconfocal morphometry the presence of OPCs in contact with brain microvessels, during postnatal cerebral cortex vascularization at postnatal day 6, in wild-type (WT) and NG2 knock-out (NG2KO) mice, and in the cortex of adult naïve and EAE-affected WT and NG2KO mice.
30870435	3	22	from	presence	662:669	arg1	NG2					806:808	NG2	806:808	NG2	806:808	To explore whether OPCs may actually be recruited within the neurovascular unit (NVU), de facto intervening in its cellular and molecular composition, we quantified by immunoconfocal morphometry the presence of OPCs in contact with brain microvessels, during postnatal cerebral cortex vascularization at postnatal day 6, in wild-type (WT) and NG2 knock-out (NG2KO) mice, and in the cortex of adult naïve and EAE-affected WT and NG2KO mice.
30870435	3	22	from	presence	662:669	arg1	contact					682:688	contact	682:688	contact with brain microvessels	682:712	To explore whether OPCs may actually be recruited within the neurovascular unit (NVU), de facto intervening in its cellular and molecular composition, we quantified by immunoconfocal morphometry the presence of OPCs in contact with brain microvessels, during postnatal cerebral cortex vascularization at postnatal day 6, in wild-type (WT) and NG2 knock-out (NG2KO) mice, and in the cortex of adult naïve and EAE-affected WT and NG2KO mice.
30870435	8	23	theme	platelet-derived	1746:1761	arg1	PDGF-AA					1781:1787	PDGF-AA	1781:1787	PDGF-AA	1781:1787	In addition, analysis of the major ligand/receptor systems known to promote OPC proliferation and migration indicated that vascular endothelial growth factor A (VEGF-A), platelet-derived growth factor-AA (PDGF-AA), and the transforming growth factor-β (TGF-β) were the molecules most likely involved in proliferation and recruitment of vascular OPCs during EAE.
30870435	8	23	theme	platelet-derived	1746:1761	arg1	factor-AA					1770:1778	platelet-derived growth factor-AA	1746:1778	platelet-derived growth factor-AA (PDGF-AA)	1746:1788	In addition, analysis of the major ligand/receptor systems known to promote OPC proliferation and migration indicated that vascular endothelial growth factor A (VEGF-A), platelet-derived growth factor-AA (PDGF-AA), and the transforming growth factor-β (TGF-β) were the molecules most likely involved in proliferation and recruitment of vascular OPCs during EAE.
30870435	9	24	from	Fgf2	1996:1999	arg1	microvessels					2056:2067	isolated cerebral cortex microvessels	2031:2067	isolated cerebral cortex microvessels	2031:2067	These results were confirmed by real time-PCR that showed Fgf2, Pdgfa and Tgfb expression on isolated cerebral cortex microvessels and by dual RNAscope-immunohistochemistry/in situ hybridization (IHC/ISH), which detected Vegfa and Vegfr2 transcripts on cerebral cortex sections.
30870435	4	25	theme	WT	1076:1077	arg1	mice					1079:1082	adult naïve WT mice	1064:1082	adult naïve WT mice	1064:1082	As observed in WT mice during postnatal development, a higher number of juxtavascular and perivascular OPCs was revealed in adult WT mice during EAE compared to adult naïve WT mice.
30870435	5	26	theme	altered	1165:1171	arg1	patterns					1225:1232	altered claudin-5 and occludin tight junction (TJ) staining patterns	1165:1232	altered claudin-5 and occludin tight junction (TJ) staining patterns	1165:1232	In EAE-affected mice, OPCs were mostly associated with microvessels that showed altered claudin-5 and occludin tight junction (TJ) staining patterns and barrier leakage.
30870435	3	27	theme	postnatal	767:775	arg1	day					777:779	postnatal day 6	767:781	postnatal day 6	767:781	To explore whether OPCs may actually be recruited within the neurovascular unit (NVU), de facto intervening in its cellular and molecular composition, we quantified by immunoconfocal morphometry the presence of OPCs in contact with brain microvessels, during postnatal cerebral cortex vascularization at postnatal day 6, in wild-type (WT) and NG2 knock-out (NG2KO) mice, and in the cortex of adult naïve and EAE-affected WT and NG2KO mice.
30870435	9	28	from	expression	2017:2026	arg1	microvessels					2056:2067	isolated cerebral cortex microvessels	2031:2067	isolated cerebral cortex microvessels	2031:2067	These results were confirmed by real time-PCR that showed Fgf2, Pdgfa and Tgfb expression on isolated cerebral cortex microvessels and by dual RNAscope-immunohistochemistry/in situ hybridization (IHC/ISH), which detected Vegfa and Vegfr2 transcripts on cerebral cortex sections.
30870435	9	29	theme	cerebral	2191:2198	arg1	sections					2207:2214	cerebral cortex sections	2191:2214	cerebral cortex sections	2191:2214	These results were confirmed by real time-PCR that showed Fgf2, Pdgfa and Tgfb expression on isolated cerebral cortex microvessels and by dual RNAscope-immunohistochemistry/in situ hybridization (IHC/ISH), which detected Vegfa and Vegfr2 transcripts on cerebral cortex sections.
30870435	2	30	with	contacts	427:434	arg1	wall					457:460	the microvessel wall	441:460	the microvessel wall	441:460	During experimental autoimmune encephalomyelitis (EAE), a model for multiple sclerosis associated with blood-brain barrier (BBB) disruption, oligodendrocyte precursor cells (OPCs) overexpress proteoglycan nerve/glial antigen 2 (NG2), proliferate, and make contacts with the microvessel wall.
30870435	1	31	theme	unit	134:137	arg1	analysis					104:111	A biofunctional analysis	88:111	A biofunctional analysis of the neurovascular unit in wild type and NG2 null mice	88:168	A biofunctional analysis of the neurovascular unit in wild type and NG2 null mice.
30870435	1	32	theme	neurovascular	120:132	arg1	unit					134:137	the neurovascular unit	116:137	the neurovascular unit in wild type and NG2 null mice	116:168	A biofunctional analysis of the neurovascular unit in wild type and NG2 null mice.
30870435	8	33	theme	major	1605:1609	arg1	systems					1627:1633	the major ligand/receptor systems	1601:1633	the major ligand/receptor systems known to promote OPC proliferation and migration indicated that vascular endothelial growth factor A (VEGF-A), platelet-derived growth factor-AA (PDGF-AA), and the transforming growth factor-β (TGF-β)	1601:1834	In addition, analysis of the major ligand/receptor systems known to promote OPC proliferation and migration indicated that vascular endothelial growth factor A (VEGF-A), platelet-derived growth factor-AA (PDGF-AA), and the transforming growth factor-β (TGF-β) were the molecules most likely involved in proliferation and recruitment of vascular OPCs during EAE.
30870435	1	34	theme	wild	142:145	arg1	type					147:150	wild type	142:150	wild type	142:150	A biofunctional analysis of the neurovascular unit in wild type and NG2 null mice.
30870435	7	35	theme	NG2	1445:1447	arg1	absence					1434:1440	absence	1434:1440	absence of NG2	1434:1447	As expected, absence of NG2, in both OPCs and pericytes, led to a reduced content of vessel basal lamina molecules, laminin, collagen VI, and collagen IV.
30870435	0	36	from	role	13:16	arg1	models					58:63	experimental models	45:63	experimental models of multiple sclerosis	45:85	Defining the role of NG2-expressing cells in experimental models of multiple sclerosis.
30870435	9	37	theme	cerebral	2040:2047	arg1	microvessels					2056:2067	isolated cerebral cortex microvessels	2031:2067	isolated cerebral cortex microvessels	2031:2067	These results were confirmed by real time-PCR that showed Fgf2, Pdgfa and Tgfb expression on isolated cerebral cortex microvessels and by dual RNAscope-immunohistochemistry/in situ hybridization (IHC/ISH), which detected Vegfa and Vegfr2 transcripts on cerebral cortex sections.
30870435	0	38	theme	multiple	68:75	arg1	sclerosis					77:85	multiple sclerosis	68:85	multiple sclerosis	68:85	Defining the role of NG2-expressing cells in experimental models of multiple sclerosis.
30870435	8	39	theme	systems	1627:1633	arg1	molecules					1845:1853	the molecules	1841:1853	the molecules most likely involved in proliferation and recruitment of vascular OPCs during EAE	1841:1935	In addition, analysis of the major ligand/receptor systems known to promote OPC proliferation and migration indicated that vascular endothelial growth factor A (VEGF-A), platelet-derived growth factor-AA (PDGF-AA), and the transforming growth factor-β (TGF-β) were the molecules most likely involved in proliferation and recruitment of vascular OPCs during EAE.
30870435	8	39	theme	systems	1627:1633	arg1	analysis					1589:1596	analysis	1589:1596	analysis of the major ligand/receptor systems known to promote OPC proliferation and migration indicated that vascular endothelial growth factor A (VEGF-A), platelet-derived growth factor-AA (PDGF-AA), and the transforming growth factor-β (TGF-β)	1589:1834	In addition, analysis of the major ligand/receptor systems known to promote OPC proliferation and migration indicated that vascular endothelial growth factor A (VEGF-A), platelet-derived growth factor-AA (PDGF-AA), and the transforming growth factor-β (TGF-β) were the molecules most likely involved in proliferation and recruitment of vascular OPCs during EAE.
30870435	1	40	from	mice	165:168	arg1	analysis					104:111	A biofunctional analysis	88:111	A biofunctional analysis of the neurovascular unit in wild type and NG2 null mice	88:168	A biofunctional analysis of the neurovascular unit in wild type and NG2 null mice.
30870435	7	41	theme	molecules	1526:1534	arg1	content					1495:1501	a reduced content	1485:1501	a reduced content of vessel basal lamina molecules, laminin, collagen VI, and collagen IV	1485:1573	As expected, absence of NG2, in both OPCs and pericytes, led to a reduced content of vessel basal lamina molecules, laminin, collagen VI, and collagen IV.
30870435	11	42	theme	BBB	2507:2509	arg1	development					2511:2521	BBB development	2507:2521	BBB development	2507:2521	Moreover, the synchronized activation of vascular OPCs and pericytes during both BBB development and dysfunction, points to NG2 as a key regulator of vascular interactions.
30870435	10	43	theme	NVU	2405:2407	arg1	components					2414:2423	the classical NVU cell components	2391:2423	the classical NVU cell components	2391:2423	Overall, this study suggests that vascular OPCs, in virtue of their developmental arrangement and response to neuroinflammation and growth factors, could be integrated among the classical NVU cell components.
30870435	8	44	theme	growth	1812:1817	arg1	TGF-β					1829:1833	TGF-β	1829:1833	TGF-β	1829:1833	In addition, analysis of the major ligand/receptor systems known to promote OPC proliferation and migration indicated that vascular endothelial growth factor A (VEGF-A), platelet-derived growth factor-AA (PDGF-AA), and the transforming growth factor-β (TGF-β) were the molecules most likely involved in proliferation and recruitment of vascular OPCs during EAE.
30870435	8	44	theme	growth	1812:1817	arg1	factor-β					1819:1826	the transforming growth factor-β	1795:1826	the transforming growth factor-β (TGF-β)	1795:1834	In addition, analysis of the major ligand/receptor systems known to promote OPC proliferation and migration indicated that vascular endothelial growth factor A (VEGF-A), platelet-derived growth factor-AA (PDGF-AA), and the transforming growth factor-β (TGF-β) were the molecules most likely involved in proliferation and recruitment of vascular OPCs during EAE.
30870435	8	45	theme	OPC	1652:1654	arg1	proliferation					1656:1668	OPC proliferation	1652:1668	OPC proliferation	1652:1668	In addition, analysis of the major ligand/receptor systems known to promote OPC proliferation and migration indicated that vascular endothelial growth factor A (VEGF-A), platelet-derived growth factor-AA (PDGF-AA), and the transforming growth factor-β (TGF-β) were the molecules most likely involved in proliferation and recruitment of vascular OPCs during EAE.
30870435	7	46	theme	basal	1513:1517	arg1	lamina					1519:1524	vessel basal lamina	1506:1524	vessel basal lamina molecules	1506:1534	As expected, absence of NG2, in both OPCs and pericytes, led to a reduced content of vessel basal lamina molecules, laminin, collagen VI, and collagen IV.
30870435	2	47	theme	proteoglycan	363:374	arg1	antigen					388:394	proteoglycan nerve/glial antigen 2	363:396	proteoglycan nerve/glial antigen 2 (NG2)	363:402	During experimental autoimmune encephalomyelitis (EAE), a model for multiple sclerosis associated with blood-brain barrier (BBB) disruption, oligodendrocyte precursor cells (OPCs) overexpress proteoglycan nerve/glial antigen 2 (NG2), proliferate, and make contacts with the microvessel wall.
30870435	2	47	theme	proteoglycan	363:374	arg1	NG2					399:401	NG2	399:401	NG2	399:401	During experimental autoimmune encephalomyelitis (EAE), a model for multiple sclerosis associated with blood-brain barrier (BBB) disruption, oligodendrocyte precursor cells (OPCs) overexpress proteoglycan nerve/glial antigen 2 (NG2), proliferate, and make contacts with the microvessel wall.
30870435	4	48	theme	postnatal	933:941	arg1	development					943:953	postnatal development	933:953	postnatal development	933:953	As observed in WT mice during postnatal development, a higher number of juxtavascular and perivascular OPCs was revealed in adult WT mice during EAE compared to adult naïve WT mice.
30870435	11	49	theme	vascular	2467:2474	arg1	OPCs					2476:2479	vascular OPCs	2467:2479	vascular OPCs	2467:2479	Moreover, the synchronized activation of vascular OPCs and pericytes during both BBB development and dysfunction, points to NG2 as a key regulator of vascular interactions.
30870435	5	50	theme	tight	1196:1200	arg1	junction					1202:1209	occludin tight junction	1187:1209	occludin tight junction (TJ)	1187:1214	In EAE-affected mice, OPCs were mostly associated with microvessels that showed altered claudin-5 and occludin tight junction (TJ) staining patterns and barrier leakage.
30870435	5	50	theme	tight	1196:1200	arg1	TJ					1212:1213	TJ	1212:1213	TJ	1212:1213	In EAE-affected mice, OPCs were mostly associated with microvessels that showed altered claudin-5 and occludin tight junction (TJ) staining patterns and barrier leakage.
30870435	7	51	theme	reduced	1487:1493	arg1	content					1495:1501	a reduced content	1485:1501	a reduced content of vessel basal lamina molecules, laminin, collagen VI, and collagen IV	1485:1573	As expected, absence of NG2, in both OPCs and pericytes, led to a reduced content of vessel basal lamina molecules, laminin, collagen VI, and collagen IV.
30870435	0	52	theme	NG2-expressing	21:34	arg1	cells					36:40	NG2-expressing cells	21:40	NG2-expressing cells	21:40	Defining the role of NG2-expressing cells in experimental models of multiple sclerosis.
30870435	8	53	theme	vascular	1912:1919	arg1	OPCs					1921:1924	vascular OPCs	1912:1924	vascular OPCs during EAE	1912:1935	In addition, analysis of the major ligand/receptor systems known to promote OPC proliferation and migration indicated that vascular endothelial growth factor A (VEGF-A), platelet-derived growth factor-AA (PDGF-AA), and the transforming growth factor-β (TGF-β) were the molecules most likely involved in proliferation and recruitment of vascular OPCs during EAE.
30870435	2	54	theme	precursor	328:336	arg1	OPCs					345:348	OPCs	345:348	OPCs	345:348	During experimental autoimmune encephalomyelitis (EAE), a model for multiple sclerosis associated with blood-brain barrier (BBB) disruption, oligodendrocyte precursor cells (OPCs) overexpress proteoglycan nerve/glial antigen 2 (NG2), proliferate, and make contacts with the microvessel wall.
30870435	2	54	theme	precursor	328:336	arg1	cells					338:342	oligodendrocyte precursor cells	312:342	oligodendrocyte precursor cells (OPCs)	312:349	During experimental autoimmune encephalomyelitis (EAE), a model for multiple sclerosis associated with blood-brain barrier (BBB) disruption, oligodendrocyte precursor cells (OPCs) overexpress proteoglycan nerve/glial antigen 2 (NG2), proliferate, and make contacts with the microvessel wall.
30870435	6	55	theme	NG2KO	1281:1285	arg1	mice					1287:1290	EAE-affected NG2KO mice	1268:1290	EAE-affected NG2KO mice	1268:1290	In contrast, EAE-affected NG2KO mice, which did not show any significant increase in vessel-associated OPCs, seemed to retain better preserved TJs and BBB integrity.
30870435	3	56	theme	adult	855:859	arg1	WT					884:885	adult naïve and EAE-affected WT and NG2KO mice	855:900	WT	884:885	To explore whether OPCs may actually be recruited within the neurovascular unit (NVU), de facto intervening in its cellular and molecular composition, we quantified by immunoconfocal morphometry the presence of OPCs in contact with brain microvessels, during postnatal cerebral cortex vascularization at postnatal day 6, in wild-type (WT) and NG2 knock-out (NG2KO) mice, and in the cortex of adult naïve and EAE-affected WT and NG2KO mice.
30870435	2	57	theme	autoimmune	191:200	arg1	model					229:233	a model	227:233	a model for multiple sclerosis associated with blood-brain barrier (BBB) disruption	227:309	During experimental autoimmune encephalomyelitis (EAE), a model for multiple sclerosis associated with blood-brain barrier (BBB) disruption, oligodendrocyte precursor cells (OPCs) overexpress proteoglycan nerve/glial antigen 2 (NG2), proliferate, and make contacts with the microvessel wall.
30870435	2	57	theme	autoimmune	191:200	arg1	EAE					221:223	EAE	221:223	EAE	221:223	During experimental autoimmune encephalomyelitis (EAE), a model for multiple sclerosis associated with blood-brain barrier (BBB) disruption, oligodendrocyte precursor cells (OPCs) overexpress proteoglycan nerve/glial antigen 2 (NG2), proliferate, and make contacts with the microvessel wall.
30870435	2	57	theme	autoimmune	191:200	arg1	encephalomyelitis					202:218	experimental autoimmune encephalomyelitis	178:218	experimental autoimmune encephalomyelitis (EAE)	178:224	During experimental autoimmune encephalomyelitis (EAE), a model for multiple sclerosis associated with blood-brain barrier (BBB) disruption, oligodendrocyte precursor cells (OPCs) overexpress proteoglycan nerve/glial antigen 2 (NG2), proliferate, and make contacts with the microvessel wall.
30870435	1	58	from	analysis	104:111	arg1	mice					165:168	wild type and NG2 null mice	142:168	wild type and NG2 null mice	142:168	A biofunctional analysis of the neurovascular unit in wild type and NG2 null mice.
30870435	3	59	theme	NG2KO	891:895	arg1	mice					897:900	adult naïve and EAE-affected WT and NG2KO mice	855:900	mice	897:900	To explore whether OPCs may actually be recruited within the neurovascular unit (NVU), de facto intervening in its cellular and molecular composition, we quantified by immunoconfocal morphometry the presence of OPCs in contact with brain microvessels, during postnatal cerebral cortex vascularization at postnatal day 6, in wild-type (WT) and NG2 knock-out (NG2KO) mice, and in the cortex of adult naïve and EAE-affected WT and NG2KO mice.
30870435	5	60	theme	barrier	1238:1244	arg1	leakage					1246:1252	barrier leakage	1238:1252	barrier leakage	1238:1252	In EAE-affected mice, OPCs were mostly associated with microvessels that showed altered claudin-5 and occludin tight junction (TJ) staining patterns and barrier leakage.
30870435	3	61	with	contact	682:688	arg1	microvessels					701:712	brain microvessels	695:712	brain microvessels	695:712	To explore whether OPCs may actually be recruited within the neurovascular unit (NVU), de facto intervening in its cellular and molecular composition, we quantified by immunoconfocal morphometry the presence of OPCs in contact with brain microvessels, during postnatal cerebral cortex vascularization at postnatal day 6, in wild-type (WT) and NG2 knock-out (NG2KO) mice, and in the cortex of adult naïve and EAE-affected WT and NG2KO mice.
30870435	3	62	theme	WT	884:885	arg1	cortex					845:850	the cortex	841:850	the cortex of adult naïve and EAE-affected WT and NG2KO mice	841:900	To explore whether OPCs may actually be recruited within the neurovascular unit (NVU), de facto intervening in its cellular and molecular composition, we quantified by immunoconfocal morphometry the presence of OPCs in contact with brain microvessels, during postnatal cerebral cortex vascularization at postnatal day 6, in wild-type (WT) and NG2 knock-out (NG2KO) mice, and in the cortex of adult naïve and EAE-affected WT and NG2KO mice.
30870435	9	63	from	Pdgfa	2002:2006	arg1	microvessels					2056:2067	isolated cerebral cortex microvessels	2031:2067	isolated cerebral cortex microvessels	2031:2067	These results were confirmed by real time-PCR that showed Fgf2, Pdgfa and Tgfb expression on isolated cerebral cortex microvessels and by dual RNAscope-immunohistochemistry/in situ hybridization (IHC/ISH), which detected Vegfa and Vegfr2 transcripts on cerebral cortex sections.
30870435	4	64	theme	higher	958:963	arg1	number					965:970	a higher number	956:970	a higher number of juxtavascular and perivascular OPCs	956:1009	As observed in WT mice during postnatal development, a higher number of juxtavascular and perivascular OPCs was revealed in adult WT mice during EAE compared to adult naïve WT mice.
30870435	8	65	theme	vascular	1699:1706	arg1	factor					1727:1732	vascular endothelial growth factor	1699:1732	vascular endothelial growth factor A (VEGF-A)	1699:1743	In addition, analysis of the major ligand/receptor systems known to promote OPC proliferation and migration indicated that vascular endothelial growth factor A (VEGF-A), platelet-derived growth factor-AA (PDGF-AA), and the transforming growth factor-β (TGF-β) were the molecules most likely involved in proliferation and recruitment of vascular OPCs during EAE.
30870435	2	66	theme	blood-brain	274:284	arg1	disruption					300:309	blood-brain barrier (BBB) disruption	274:309	blood-brain barrier (BBB) disruption	274:309	During experimental autoimmune encephalomyelitis (EAE), a model for multiple sclerosis associated with blood-brain barrier (BBB) disruption, oligodendrocyte precursor cells (OPCs) overexpress proteoglycan nerve/glial antigen 2 (NG2), proliferate, and make contacts with the microvessel wall.
30870435	6	67	theme	significant	1316:1326	arg1	increase					1328:1335	any significant increase	1312:1335	any significant increase in vessel-associated OPCs	1312:1361	In contrast, EAE-affected NG2KO mice, which did not show any significant increase in vessel-associated OPCs, seemed to retain better preserved TJs and BBB integrity.
30870435	5	68	theme	EAE-affected	1088:1099	arg1	mice					1101:1104	EAE-affected mice	1088:1104	EAE-affected mice	1088:1104	In EAE-affected mice, OPCs were mostly associated with microvessels that showed altered claudin-5 and occludin tight junction (TJ) staining patterns and barrier leakage.
30870435	4	69	theme	adult	1027:1031	arg1	mice					1036:1039	adult WT mice	1027:1039	adult WT mice	1027:1039	As observed in WT mice during postnatal development, a higher number of juxtavascular and perivascular OPCs was revealed in adult WT mice during EAE compared to adult naïve WT mice.
30870435	8	70	theme	growth	1720:1725	arg1	factor					1727:1732	vascular endothelial growth factor	1699:1732	vascular endothelial growth factor A (VEGF-A)	1699:1743	In addition, analysis of the major ligand/receptor systems known to promote OPC proliferation and migration indicated that vascular endothelial growth factor A (VEGF-A), platelet-derived growth factor-AA (PDGF-AA), and the transforming growth factor-β (TGF-β) were the molecules most likely involved in proliferation and recruitment of vascular OPCs during EAE.
30870435	1	71	theme	NG2	156:158	arg1	mice					165:168	wild type and NG2 null mice	142:168	wild type and NG2 null mice	142:168	A biofunctional analysis of the neurovascular unit in wild type and NG2 null mice.
30870435	10	72	theme	arrangement	2299:2309	arg1	virtue					2269:2274	virtue	2269:2274	virtue of their developmental arrangement and response to neuroinflammation and growth factors	2269:2362	Overall, this study suggests that vascular OPCs, in virtue of their developmental arrangement and response to neuroinflammation and growth factors, could be integrated among the classical NVU cell components.
30870435	3	73	theme	OPCs	674:677	arg1	presence					662:669	the presence	658:669	the presence of OPCs in contact with brain microvessels, during postnatal cerebral cortex vascularization at postnatal day 6, in wild-type (WT) and NG2 knock-out (NG2KO) mice, and in the cortex of adult naïve and EAE-affected WT and NG2KO mice	658:900	To explore whether OPCs may actually be recruited within the neurovascular unit (NVU), de facto intervening in its cellular and molecular composition, we quantified by immunoconfocal morphometry the presence of OPCs in contact with brain microvessels, during postnatal cerebral cortex vascularization at postnatal day 6, in wild-type (WT) and NG2 knock-out (NG2KO) mice, and in the cortex of adult naïve and EAE-affected WT and NG2KO mice.
30870435	4	74	theme	OPCs	1006:1009	arg1	number					965:970	a higher number	956:970	a higher number of juxtavascular and perivascular OPCs	956:1009	As observed in WT mice during postnatal development, a higher number of juxtavascular and perivascular OPCs was revealed in adult WT mice during EAE compared to adult naïve WT mice.
30870435	6	75	theme	preserved	1388:1396	arg1	integrity					1410:1418	better preserved TJs and BBB integrity	1381:1418	better preserved TJs and BBB integrity	1381:1418	In contrast, EAE-affected NG2KO mice, which did not show any significant increase in vessel-associated OPCs, seemed to retain better preserved TJs and BBB integrity.
30870435	11	76	theme	interactions	2585:2596	arg1	regulator					2563:2571	a key regulator	2557:2571	a key regulator of vascular interactions	2557:2596	Moreover, the synchronized activation of vascular OPCs and pericytes during both BBB development and dysfunction, points to NG2 as a key regulator of vascular interactions.
30870435	3	77	theme	brain	695:699	arg1	microvessels					701:712	brain microvessels	695:712	brain microvessels	695:712	To explore whether OPCs may actually be recruited within the neurovascular unit (NVU), de facto intervening in its cellular and molecular composition, we quantified by immunoconfocal morphometry the presence of OPCs in contact with brain microvessels, during postnatal cerebral cortex vascularization at postnatal day 6, in wild-type (WT) and NG2 knock-out (NG2KO) mice, and in the cortex of adult naïve and EAE-affected WT and NG2KO mice.
30870435	4	78	theme	naïve	1070:1074	arg1	mice					1079:1082	adult naïve WT mice	1064:1082	adult naïve WT mice	1064:1082	As observed in WT mice during postnatal development, a higher number of juxtavascular and perivascular OPCs was revealed in adult WT mice during EAE compared to adult naïve WT mice.
30870435	10	79	theme	response	2315:2322	arg1	virtue					2269:2274	virtue	2269:2274	virtue of their developmental arrangement and response to neuroinflammation and growth factors	2269:2362	Overall, this study suggests that vascular OPCs, in virtue of their developmental arrangement and response to neuroinflammation and growth factors, could be integrated among the classical NVU cell components.
30870435	3	80	theme	cortex	741:746	arg1	vascularization					748:762	postnatal cerebral cortex vascularization	722:762	postnatal cerebral cortex vascularization at postnatal day 6	722:781	To explore whether OPCs may actually be recruited within the neurovascular unit (NVU), de facto intervening in its cellular and molecular composition, we quantified by immunoconfocal morphometry the presence of OPCs in contact with brain microvessels, during postnatal cerebral cortex vascularization at postnatal day 6, in wild-type (WT) and NG2 knock-out (NG2KO) mice, and in the cortex of adult naïve and EAE-affected WT and NG2KO mice.
30870435	8	81	theme	growth	1763:1768	arg1	PDGF-AA					1781:1787	PDGF-AA	1781:1787	PDGF-AA	1781:1787	In addition, analysis of the major ligand/receptor systems known to promote OPC proliferation and migration indicated that vascular endothelial growth factor A (VEGF-A), platelet-derived growth factor-AA (PDGF-AA), and the transforming growth factor-β (TGF-β) were the molecules most likely involved in proliferation and recruitment of vascular OPCs during EAE.
30870435	8	81	theme	growth	1763:1768	arg1	factor-AA					1770:1778	platelet-derived growth factor-AA	1746:1778	platelet-derived growth factor-AA (PDGF-AA)	1746:1788	In addition, analysis of the major ligand/receptor systems known to promote OPC proliferation and migration indicated that vascular endothelial growth factor A (VEGF-A), platelet-derived growth factor-AA (PDGF-AA), and the transforming growth factor-β (TGF-β) were the molecules most likely involved in proliferation and recruitment of vascular OPCs during EAE.
30870435	3	82	theme	postnatal	722:730	arg1	vascularization					748:762	postnatal cerebral cortex vascularization	722:762	postnatal cerebral cortex vascularization at postnatal day 6	722:781	To explore whether OPCs may actually be recruited within the neurovascular unit (NVU), de facto intervening in its cellular and molecular composition, we quantified by immunoconfocal morphometry the presence of OPCs in contact with brain microvessels, during postnatal cerebral cortex vascularization at postnatal day 6, in wild-type (WT) and NG2 knock-out (NG2KO) mice, and in the cortex of adult naïve and EAE-affected WT and NG2KO mice.
30870435	2	83	theme	multiple	239:246	arg1	sclerosis					248:256	multiple sclerosis	239:256	multiple sclerosis associated with blood-brain barrier (BBB) disruption	239:309	During experimental autoimmune encephalomyelitis (EAE), a model for multiple sclerosis associated with blood-brain barrier (BBB) disruption, oligodendrocyte precursor cells (OPCs) overexpress proteoglycan nerve/glial antigen 2 (NG2), proliferate, and make contacts with the microvessel wall.
30870435	3	84	dep	recruited	503:511	arg1	intervening					559:569	intervening	559:569	intervening in its cellular and molecular composition	559:611	To explore whether OPCs may actually be recruited within the neurovascular unit (NVU), de facto intervening in its cellular and molecular composition, we quantified by immunoconfocal morphometry the presence of OPCs in contact with brain microvessels, during postnatal cerebral cortex vascularization at postnatal day 6, in wild-type (WT) and NG2 knock-out (NG2KO) mice, and in the cortex of adult naïve and EAE-affected WT and NG2KO mice.
30870435	10	85	theme	growth	2349:2354	arg1	factors					2356:2362	growth factors	2349:2362	growth factors	2349:2362	Overall, this study suggests that vascular OPCs, in virtue of their developmental arrangement and response to neuroinflammation and growth factors, could be integrated among the classical NVU cell components.
30870435	5	86	theme	claudin-5	1173:1181	arg1	patterns					1225:1232	altered claudin-5 and occludin tight junction (TJ) staining patterns	1165:1232	altered claudin-5 and occludin tight junction (TJ) staining patterns	1165:1232	In EAE-affected mice, OPCs were mostly associated with microvessels that showed altered claudin-5 and occludin tight junction (TJ) staining patterns and barrier leakage.
30870435	6	87	theme	EAE-affected	1268:1279	arg1	mice					1287:1290	EAE-affected NG2KO mice	1268:1290	EAE-affected NG2KO mice	1268:1290	In contrast, EAE-affected NG2KO mice, which did not show any significant increase in vessel-associated OPCs, seemed to retain better preserved TJs and BBB integrity.
30870435	3	88	dep	wild-type	787:795	arg1	mice					828:831	knock-out (NG2KO) mice	810:831	knock-out (NG2KO) mice	810:831	To explore whether OPCs may actually be recruited within the neurovascular unit (NVU), de facto intervening in its cellular and molecular composition, we quantified by immunoconfocal morphometry the presence of OPCs in contact with brain microvessels, during postnatal cerebral cortex vascularization at postnatal day 6, in wild-type (WT) and NG2 knock-out (NG2KO) mice, and in the cortex of adult naïve and EAE-affected WT and NG2KO mice.
30870435	9	89	theme	isolated	2031:2038	arg1	microvessels					2056:2067	isolated cerebral cortex microvessels	2031:2067	isolated cerebral cortex microvessels	2031:2067	These results were confirmed by real time-PCR that showed Fgf2, Pdgfa and Tgfb expression on isolated cerebral cortex microvessels and by dual RNAscope-immunohistochemistry/in situ hybridization (IHC/ISH), which detected Vegfa and Vegfr2 transcripts on cerebral cortex sections.
30870435	0	90	theme	sclerosis	77:85	arg1	models					58:63	experimental models	45:63	experimental models of multiple sclerosis	45:85	Defining the role of NG2-expressing cells in experimental models of multiple sclerosis.
30870435	9	91	theme	dual	2076:2079	arg1	IHC/ISH					2134:2140	IHC/ISH	2134:2140	IHC/ISH	2134:2140	These results were confirmed by real time-PCR that showed Fgf2, Pdgfa and Tgfb expression on isolated cerebral cortex microvessels and by dual RNAscope-immunohistochemistry/in situ hybridization (IHC/ISH), which detected Vegfa and Vegfr2 transcripts on cerebral cortex sections.
30870435	9	91	theme	dual	2076:2079	arg1	hybridization					2119:2131	dual RNAscope-immunohistochemistry/in situ hybridization	2076:2131	dual RNAscope-immunohistochemistry/in situ hybridization (IHC/ISH)	2076:2141	These results were confirmed by real time-PCR that showed Fgf2, Pdgfa and Tgfb expression on isolated cerebral cortex microvessels and by dual RNAscope-immunohistochemistry/in situ hybridization (IHC/ISH), which detected Vegfa and Vegfr2 transcripts on cerebral cortex sections.
30870435	1	92	theme	type	147:150	arg1	mice					165:168	wild type and NG2 null mice	142:168	wild type and NG2 null mice	142:168	A biofunctional analysis of the neurovascular unit in wild type and NG2 null mice.
30870435	3	93	theme	NG2KO	821:825	arg1	mice					828:831	knock-out (NG2KO) mice	810:831	knock-out (NG2KO) mice	810:831	To explore whether OPCs may actually be recruited within the neurovascular unit (NVU), de facto intervening in its cellular and molecular composition, we quantified by immunoconfocal morphometry the presence of OPCs in contact with brain microvessels, during postnatal cerebral cortex vascularization at postnatal day 6, in wild-type (WT) and NG2 knock-out (NG2KO) mice, and in the cortex of adult naïve and EAE-affected WT and NG2KO mice.
30870435	2	94	theme	microvessel	445:455	arg1	wall					457:460	the microvessel wall	441:460	the microvessel wall	441:460	During experimental autoimmune encephalomyelitis (EAE), a model for multiple sclerosis associated with blood-brain barrier (BBB) disruption, oligodendrocyte precursor cells (OPCs) overexpress proteoglycan nerve/glial antigen 2 (NG2), proliferate, and make contacts with the microvessel wall.
30870435	10	95	from	OPCs	2260:2263	arg1	virtue					2269:2274	virtue	2269:2274	virtue of their developmental arrangement and response to neuroinflammation and growth factors	2269:2362	Overall, this study suggests that vascular OPCs, in virtue of their developmental arrangement and response to neuroinflammation and growth factors, could be integrated among the classical NVU cell components.
30870435	9	96	theme	cortex	2049:2054	arg1	microvessels					2056:2067	isolated cerebral cortex microvessels	2031:2067	isolated cerebral cortex microvessels	2031:2067	These results were confirmed by real time-PCR that showed Fgf2, Pdgfa and Tgfb expression on isolated cerebral cortex microvessels and by dual RNAscope-immunohistochemistry/in situ hybridization (IHC/ISH), which detected Vegfa and Vegfr2 transcripts on cerebral cortex sections.
30870435	8	97	theme	transforming	1799:1810	arg1	TGF-β					1829:1833	TGF-β	1829:1833	TGF-β	1829:1833	In addition, analysis of the major ligand/receptor systems known to promote OPC proliferation and migration indicated that vascular endothelial growth factor A (VEGF-A), platelet-derived growth factor-AA (PDGF-AA), and the transforming growth factor-β (TGF-β) were the molecules most likely involved in proliferation and recruitment of vascular OPCs during EAE.
30870435	8	97	theme	transforming	1799:1810	arg1	factor-β					1819:1826	the transforming growth factor-β	1795:1826	the transforming growth factor-β (TGF-β)	1795:1834	In addition, analysis of the major ligand/receptor systems known to promote OPC proliferation and migration indicated that vascular endothelial growth factor A (VEGF-A), platelet-derived growth factor-AA (PDGF-AA), and the transforming growth factor-β (TGF-β) were the molecules most likely involved in proliferation and recruitment of vascular OPCs during EAE.
30870435	8	98	theme	ligand/receptor	1611:1625	arg1	systems					1627:1633	the major ligand/receptor systems	1601:1633	the major ligand/receptor systems known to promote OPC proliferation and migration indicated that vascular endothelial growth factor A (VEGF-A), platelet-derived growth factor-AA (PDGF-AA), and the transforming growth factor-β (TGF-β)	1601:1834	In addition, analysis of the major ligand/receptor systems known to promote OPC proliferation and migration indicated that vascular endothelial growth factor A (VEGF-A), platelet-derived growth factor-AA (PDGF-AA), and the transforming growth factor-β (TGF-β) were the molecules most likely involved in proliferation and recruitment of vascular OPCs during EAE.
30870435	3	99	theme	knock-out	810:818	arg1	mice					828:831	knock-out (NG2KO) mice	810:831	knock-out (NG2KO) mice	810:831	To explore whether OPCs may actually be recruited within the neurovascular unit (NVU), de facto intervening in its cellular and molecular composition, we quantified by immunoconfocal morphometry the presence of OPCs in contact with brain microvessels, during postnatal cerebral cortex vascularization at postnatal day 6, in wild-type (WT) and NG2 knock-out (NG2KO) mice, and in the cortex of adult naïve and EAE-affected WT and NG2KO mice.
30870435	10	100	theme	cell	2409:2412	arg1	components					2414:2423	the classical NVU cell components	2391:2423	the classical NVU cell components	2391:2423	Overall, this study suggests that vascular OPCs, in virtue of their developmental arrangement and response to neuroinflammation and growth factors, could be integrated among the classical NVU cell components.
30870435	8	101	from	molecules	1845:1853	arg1	addition					1579:1586	addition	1579:1586	addition	1579:1586	In addition, analysis of the major ligand/receptor systems known to promote OPC proliferation and migration indicated that vascular endothelial growth factor A (VEGF-A), platelet-derived growth factor-AA (PDGF-AA), and the transforming growth factor-β (TGF-β) were the molecules most likely involved in proliferation and recruitment of vascular OPCs during EAE.
30870435	10	102	theme	classical	2395:2403	arg1	components					2414:2423	the classical NVU cell components	2391:2423	the classical NVU cell components	2391:2423	Overall, this study suggests that vascular OPCs, in virtue of their developmental arrangement and response to neuroinflammation and growth factors, could be integrated among the classical NVU cell components.
30870435	5	103	theme	staining	1216:1223	arg1	patterns					1225:1232	altered claudin-5 and occludin tight junction (TJ) staining patterns	1165:1232	altered claudin-5 and occludin tight junction (TJ) staining patterns	1165:1232	In EAE-affected mice, OPCs were mostly associated with microvessels that showed altered claudin-5 and occludin tight junction (TJ) staining patterns and barrier leakage.
30870435	2	104	theme	nerve/glial	376:386	arg1	antigen					388:394	proteoglycan nerve/glial antigen 2	363:396	proteoglycan nerve/glial antigen 2 (NG2)	363:402	During experimental autoimmune encephalomyelitis (EAE), a model for multiple sclerosis associated with blood-brain barrier (BBB) disruption, oligodendrocyte precursor cells (OPCs) overexpress proteoglycan nerve/glial antigen 2 (NG2), proliferate, and make contacts with the microvessel wall.
30870435	2	104	theme	nerve/glial	376:386	arg1	NG2					399:401	NG2	399:401	NG2	399:401	During experimental autoimmune encephalomyelitis (EAE), a model for multiple sclerosis associated with blood-brain barrier (BBB) disruption, oligodendrocyte precursor cells (OPCs) overexpress proteoglycan nerve/glial antigen 2 (NG2), proliferate, and make contacts with the microvessel wall.
30870435	1	105	from	unit	134:137	arg1	mice					165:168	wild type and NG2 null mice	142:168	wild type and NG2 null mice	142:168	A biofunctional analysis of the neurovascular unit in wild type and NG2 null mice.
30870435	7	106	theme	lamina	1519:1524	arg1	collagen					1546:1553	collagen VI	1546:1556	collagen VI	1546:1556	As expected, absence of NG2, in both OPCs and pericytes, led to a reduced content of vessel basal lamina molecules, laminin, collagen VI, and collagen IV.
30870435	7	106	theme	lamina	1519:1524	arg1	collagen					1563:1570	collagen IV	1563:1573	collagen IV	1563:1573	As expected, absence of NG2, in both OPCs and pericytes, led to a reduced content of vessel basal lamina molecules, laminin, collagen VI, and collagen IV.
30870435	7	106	theme	lamina	1519:1524	arg1	laminin					1537:1543	laminin	1537:1543	laminin	1537:1543	As expected, absence of NG2, in both OPCs and pericytes, led to a reduced content of vessel basal lamina molecules, laminin, collagen VI, and collagen IV.
30870435	7	106	theme	lamina	1519:1524	arg1	molecules					1526:1534	vessel basal lamina molecules	1506:1534	vessel basal lamina molecules	1506:1534	As expected, absence of NG2, in both OPCs and pericytes, led to a reduced content of vessel basal lamina molecules, laminin, collagen VI, and collagen IV.
30870435	4	107	theme	WT	918:919	arg1	mice					921:924	WT mice	918:924	WT mice	918:924	As observed in WT mice during postnatal development, a higher number of juxtavascular and perivascular OPCs was revealed in adult WT mice during EAE compared to adult naïve WT mice.
30870435	7	108	dep	collagen	1546:1553	arg1	VI					1555:1556	VI	1555:1556	VI	1555:1556	As expected, absence of NG2, in both OPCs and pericytes, led to a reduced content of vessel basal lamina molecules, laminin, collagen VI, and collagen IV.
30870435	5	109	theme	occludin	1187:1194	arg1	junction					1202:1209	occludin tight junction	1187:1209	occludin tight junction (TJ)	1187:1214	In EAE-affected mice, OPCs were mostly associated with microvessels that showed altered claudin-5 and occludin tight junction (TJ) staining patterns and barrier leakage.
30870435	5	109	theme	occludin	1187:1194	arg1	TJ					1212:1213	TJ	1212:1213	TJ	1212:1213	In EAE-affected mice, OPCs were mostly associated with microvessels that showed altered claudin-5 and occludin tight junction (TJ) staining patterns and barrier leakage.
30870435	9	110	theme	situ	2114:2117	arg1	IHC/ISH					2134:2140	IHC/ISH	2134:2140	IHC/ISH	2134:2140	These results were confirmed by real time-PCR that showed Fgf2, Pdgfa and Tgfb expression on isolated cerebral cortex microvessels and by dual RNAscope-immunohistochemistry/in situ hybridization (IHC/ISH), which detected Vegfa and Vegfr2 transcripts on cerebral cortex sections.
30870435	9	110	theme	situ	2114:2117	arg1	hybridization					2119:2131	dual RNAscope-immunohistochemistry/in situ hybridization	2076:2131	dual RNAscope-immunohistochemistry/in situ hybridization (IHC/ISH)	2076:2141	These results were confirmed by real time-PCR that showed Fgf2, Pdgfa and Tgfb expression on isolated cerebral cortex microvessels and by dual RNAscope-immunohistochemistry/in situ hybridization (IHC/ISH), which detected Vegfa and Vegfr2 transcripts on cerebral cortex sections.
30870435	1	111	theme	biofunctional	90:102	arg1	analysis					104:111	A biofunctional analysis	88:111	A biofunctional analysis of the neurovascular unit in wild type and NG2 null mice	88:168	A biofunctional analysis of the neurovascular unit in wild type and NG2 null mice.
30870435	7	112	theme	vessel	1506:1511	arg1	lamina					1519:1524	vessel basal lamina	1506:1524	vessel basal lamina molecules	1506:1534	As expected, absence of NG2, in both OPCs and pericytes, led to a reduced content of vessel basal lamina molecules, laminin, collagen VI, and collagen IV.
30870435	11	113	theme	synchronized	2440:2451	arg1	activation					2453:2462	the synchronized activation	2436:2462	the synchronized activation of vascular OPCs and pericytes	2436:2493	Moreover, the synchronized activation of vascular OPCs and pericytes during both BBB development and dysfunction, points to NG2 as a key regulator of vascular interactions.
30870435	3	114	theme	molecular	591:599	arg1	composition					601:611	its cellular and molecular composition	574:611	its cellular and molecular composition	574:611	To explore whether OPCs may actually be recruited within the neurovascular unit (NVU), de facto intervening in its cellular and molecular composition, we quantified by immunoconfocal morphometry the presence of OPCs in contact with brain microvessels, during postnatal cerebral cortex vascularization at postnatal day 6, in wild-type (WT) and NG2 knock-out (NG2KO) mice, and in the cortex of adult naïve and EAE-affected WT and NG2KO mice.
30870435	5	115	theme	junction	1202:1209	arg1	patterns					1225:1232	altered claudin-5 and occludin tight junction (TJ) staining patterns	1165:1232	altered claudin-5 and occludin tight junction (TJ) staining patterns	1165:1232	In EAE-affected mice, OPCs were mostly associated with microvessels that showed altered claudin-5 and occludin tight junction (TJ) staining patterns and barrier leakage.
30870435	0	116	theme	cells	36:40	arg1	role					13:16	the role	9:16	the role of NG2-expressing cells in experimental models of multiple sclerosis	9:85	Defining the role of NG2-expressing cells in experimental models of multiple sclerosis.
30870435	8	117	link	platelet-derived	1746:1761	arg1	PDGF-AA					1781:1787	PDGF-AA	1781:1787	PDGF-AA	1781:1787	In addition, analysis of the major ligand/receptor systems known to promote OPC proliferation and migration indicated that vascular endothelial growth factor A (VEGF-A), platelet-derived growth factor-AA (PDGF-AA), and the transforming growth factor-β (TGF-β) were the molecules most likely involved in proliferation and recruitment of vascular OPCs during EAE.
30870435	8	117	link	platelet-derived	1746:1761	arg1	factor-AA					1770:1778	platelet-derived growth factor-AA	1746:1778	platelet-derived growth factor-AA (PDGF-AA)	1746:1788	In addition, analysis of the major ligand/receptor systems known to promote OPC proliferation and migration indicated that vascular endothelial growth factor A (VEGF-A), platelet-derived growth factor-AA (PDGF-AA), and the transforming growth factor-β (TGF-β) were the molecules most likely involved in proliferation and recruitment of vascular OPCs during EAE.
30870435	8	118	theme	OPCs	1921:1924	arg1	proliferation					1879:1891	proliferation	1879:1891	proliferation	1879:1891	In addition, analysis of the major ligand/receptor systems known to promote OPC proliferation and migration indicated that vascular endothelial growth factor A (VEGF-A), platelet-derived growth factor-AA (PDGF-AA), and the transforming growth factor-β (TGF-β) were the molecules most likely involved in proliferation and recruitment of vascular OPCs during EAE.
30870435	8	118	theme	OPCs	1921:1924	arg1	recruitment					1897:1907	recruitment	1897:1907	recruitment	1897:1907	In addition, analysis of the major ligand/receptor systems known to promote OPC proliferation and migration indicated that vascular endothelial growth factor A (VEGF-A), platelet-derived growth factor-AA (PDGF-AA), and the transforming growth factor-β (TGF-β) were the molecules most likely involved in proliferation and recruitment of vascular OPCs during EAE.
30870435	11	119	theme	pericytes	2485:2493	arg1	activation					2453:2462	the synchronized activation	2436:2462	the synchronized activation of vascular OPCs and pericytes	2436:2493	Moreover, the synchronized activation of vascular OPCs and pericytes during both BBB development and dysfunction, points to NG2 as a key regulator of vascular interactions.
30870435	3	120	theme	cellular	578:585	arg1	composition					601:611	its cellular and molecular composition	574:611	its cellular and molecular composition	574:611	To explore whether OPCs may actually be recruited within the neurovascular unit (NVU), de facto intervening in its cellular and molecular composition, we quantified by immunoconfocal morphometry the presence of OPCs in contact with brain microvessels, during postnatal cerebral cortex vascularization at postnatal day 6, in wild-type (WT) and NG2 knock-out (NG2KO) mice, and in the cortex of adult naïve and EAE-affected WT and NG2KO mice.
30870435	0	121	theme	experimental	45:56	arg1	models					58:63	experimental models	45:63	experimental models of multiple sclerosis	45:85	Defining the role of NG2-expressing cells in experimental models of multiple sclerosis.
30870435	3	122	theme	EAE-affected	871:882	arg1	WT					884:885	adult naïve and EAE-affected WT and NG2KO mice	855:900	WT	884:885	To explore whether OPCs may actually be recruited within the neurovascular unit (NVU), de facto intervening in its cellular and molecular composition, we quantified by immunoconfocal morphometry the presence of OPCs in contact with brain microvessels, during postnatal cerebral cortex vascularization at postnatal day 6, in wild-type (WT) and NG2 knock-out (NG2KO) mice, and in the cortex of adult naïve and EAE-affected WT and NG2KO mice.
30870435	11	123	theme	OPCs	2476:2479	arg1	activation					2453:2462	the synchronized activation	2436:2462	the synchronized activation of vascular OPCs and pericytes	2436:2493	Moreover, the synchronized activation of vascular OPCs and pericytes during both BBB development and dysfunction, points to NG2 as a key regulator of vascular interactions.
30870435	2	124	theme	oligodendrocyte	312:326	arg1	OPCs					345:348	OPCs	345:348	OPCs	345:348	During experimental autoimmune encephalomyelitis (EAE), a model for multiple sclerosis associated with blood-brain barrier (BBB) disruption, oligodendrocyte precursor cells (OPCs) overexpress proteoglycan nerve/glial antigen 2 (NG2), proliferate, and make contacts with the microvessel wall.
30870435	2	124	theme	oligodendrocyte	312:326	arg1	cells					338:342	oligodendrocyte precursor cells	312:342	oligodendrocyte precursor cells (OPCs)	312:349	During experimental autoimmune encephalomyelitis (EAE), a model for multiple sclerosis associated with blood-brain barrier (BBB) disruption, oligodendrocyte precursor cells (OPCs) overexpress proteoglycan nerve/glial antigen 2 (NG2), proliferate, and make contacts with the microvessel wall.
30870435	2	125	theme	barrier	286:292	arg1	disruption					300:309	blood-brain barrier (BBB) disruption	274:309	blood-brain barrier (BBB) disruption	274:309	During experimental autoimmune encephalomyelitis (EAE), a model for multiple sclerosis associated with blood-brain barrier (BBB) disruption, oligodendrocyte precursor cells (OPCs) overexpress proteoglycan nerve/glial antigen 2 (NG2), proliferate, and make contacts with the microvessel wall.
30870435	6	126	from	increase	1328:1335	arg1	OPCs					1358:1361	vessel-associated OPCs	1340:1361	vessel-associated OPCs	1340:1361	In contrast, EAE-affected NG2KO mice, which did not show any significant increase in vessel-associated OPCs, seemed to retain better preserved TJs and BBB integrity.
30870435	3	127	theme	naïve	861:865	arg1	WT					884:885	adult naïve and EAE-affected WT and NG2KO mice	855:900	WT	884:885	To explore whether OPCs may actually be recruited within the neurovascular unit (NVU), de facto intervening in its cellular and molecular composition, we quantified by immunoconfocal morphometry the presence of OPCs in contact with brain microvessels, during postnatal cerebral cortex vascularization at postnatal day 6, in wild-type (WT) and NG2 knock-out (NG2KO) mice, and in the cortex of adult naïve and EAE-affected WT and NG2KO mice.
30870435	3	128	from	day	777:779	arg1	vascularization					748:762	postnatal cerebral cortex vascularization	722:762	postnatal cerebral cortex vascularization at postnatal day 6	722:781	To explore whether OPCs may actually be recruited within the neurovascular unit (NVU), de facto intervening in its cellular and molecular composition, we quantified by immunoconfocal morphometry the presence of OPCs in contact with brain microvessels, during postnatal cerebral cortex vascularization at postnatal day 6, in wild-type (WT) and NG2 knock-out (NG2KO) mice, and in the cortex of adult naïve and EAE-affected WT and NG2KO mice.
30870435	2	129	theme	experimental	178:189	arg1	model					229:233	a model	227:233	a model for multiple sclerosis associated with blood-brain barrier (BBB) disruption	227:309	During experimental autoimmune encephalomyelitis (EAE), a model for multiple sclerosis associated with blood-brain barrier (BBB) disruption, oligodendrocyte precursor cells (OPCs) overexpress proteoglycan nerve/glial antigen 2 (NG2), proliferate, and make contacts with the microvessel wall.
30870435	2	129	theme	experimental	178:189	arg1	EAE					221:223	EAE	221:223	EAE	221:223	During experimental autoimmune encephalomyelitis (EAE), a model for multiple sclerosis associated with blood-brain barrier (BBB) disruption, oligodendrocyte precursor cells (OPCs) overexpress proteoglycan nerve/glial antigen 2 (NG2), proliferate, and make contacts with the microvessel wall.
30870435	2	129	theme	experimental	178:189	arg1	encephalomyelitis					202:218	experimental autoimmune encephalomyelitis	178:218	experimental autoimmune encephalomyelitis (EAE)	178:224	During experimental autoimmune encephalomyelitis (EAE), a model for multiple sclerosis associated with blood-brain barrier (BBB) disruption, oligodendrocyte precursor cells (OPCs) overexpress proteoglycan nerve/glial antigen 2 (NG2), proliferate, and make contacts with the microvessel wall.
30870435	9	130	theme	Vegfa	2159:2163	arg1	transcripts					2176:2186	Vegfa and Vegfr2 transcripts	2159:2186	Vegfa and Vegfr2 transcripts	2159:2186	These results were confirmed by real time-PCR that showed Fgf2, Pdgfa and Tgfb expression on isolated cerebral cortex microvessels and by dual RNAscope-immunohistochemistry/in situ hybridization (IHC/ISH), which detected Vegfa and Vegfr2 transcripts on cerebral cortex sections.
30870435	3	131	theme	mice	897:900	arg1	cortex					845:850	the cortex	841:850	the cortex of adult naïve and EAE-affected WT and NG2KO mice	841:900	To explore whether OPCs may actually be recruited within the neurovascular unit (NVU), de facto intervening in its cellular and molecular composition, we quantified by immunoconfocal morphometry the presence of OPCs in contact with brain microvessels, during postnatal cerebral cortex vascularization at postnatal day 6, in wild-type (WT) and NG2 knock-out (NG2KO) mice, and in the cortex of adult naïve and EAE-affected WT and NG2KO mice.
30870435	4	132	theme	juxtavascular	975:987	arg1	OPCs					1006:1009	juxtavascular and perivascular OPCs	975:1009	juxtavascular and perivascular OPCs	975:1009	As observed in WT mice during postnatal development, a higher number of juxtavascular and perivascular OPCs was revealed in adult WT mice during EAE compared to adult naïve WT mice.
30870435	9	133	theme	Vegfr2	2169:2174	arg1	transcripts					2176:2186	Vegfa and Vegfr2 transcripts	2159:2186	Vegfa and Vegfr2 transcripts	2159:2186	These results were confirmed by real time-PCR that showed Fgf2, Pdgfa and Tgfb expression on isolated cerebral cortex microvessels and by dual RNAscope-immunohistochemistry/in situ hybridization (IHC/ISH), which detected Vegfa and Vegfr2 transcripts on cerebral cortex sections.
30870435	6	134	theme	vessel-associated	1340:1356	arg1	OPCs					1358:1361	vessel-associated OPCs	1340:1361	vessel-associated OPCs	1340:1361	In contrast, EAE-affected NG2KO mice, which did not show any significant increase in vessel-associated OPCs, seemed to retain better preserved TJs and BBB integrity.
31226431	3	0	theme	common	652:657	arg1	infusion					638:645	the infusion	634:645	the infusion	634:645	It was verified that thermal treatment affected the contents of vitamin C and carotenoids, but hardly those of polyphenols, which would be the main bioactive compounds in the infusion, the common form of preparation of horchata for consumption.
31226431	3	0	theme	common	652:657	arg1	form					659:662	the common form	648:662	the common form of preparation of horchata for consumption	648:705	It was verified that thermal treatment affected the contents of vitamin C and carotenoids, but hardly those of polyphenols, which would be the main bioactive compounds in the infusion, the common form of preparation of horchata for consumption.
31226431	2	1	theme	mixture	454:460	arg1	IHext					425:429	IHext	425:429	IHext	425:429	The study was carried out using the hydroalcoholic extract (HHext) and infusion (IHext) of the horchata plant mixture.
31226431	2	1	theme	mixture	454:460	arg1	HHext					404:408	HHext	404:408	HHext	404:408	The study was carried out using the hydroalcoholic extract (HHext) and infusion (IHext) of the horchata plant mixture.
31226431	2	1	theme	mixture	454:460	arg1	infusion					415:422	infusion	415:422	infusion (IHext)	415:430	The study was carried out using the hydroalcoholic extract (HHext) and infusion (IHext) of the horchata plant mixture.
31226431	2	1	theme	mixture	454:460	arg1	extract					395:401	the hydroalcoholic extract	376:401	the hydroalcoholic extract (HHext)	376:409	The study was carried out using the hydroalcoholic extract (HHext) and infusion (IHext) of the horchata plant mixture.
31226431	3	2	theme	bioactive	611:619	arg1	compounds					621:629	the main bioactive compounds	602:629	the main bioactive compounds	602:629	It was verified that thermal treatment affected the contents of vitamin C and carotenoids, but hardly those of polyphenols, which would be the main bioactive compounds in the infusion, the common form of preparation of horchata for consumption.
31226431	3	2	theme	bioactive	611:619	arg1	polyphenols					574:584	polyphenols	574:584	polyphenols	574:584	It was verified that thermal treatment affected the contents of vitamin C and carotenoids, but hardly those of polyphenols, which would be the main bioactive compounds in the infusion, the common form of preparation of horchata for consumption.
31226431	6	3	theme	protective	1081:1090	arg1	effects					1092:1098	Greater protective effects	1073:1098	Greater protective effects	1073:1098	Greater protective effects were obtained for HHext compared to IHext, which was in agreement with its higher content of phenolic compounds favoured by a more efficient extraction in the hydroalcoholic medium.
31226431	1	4	theme	plants	264:269	arg1	mixture					250:256	a mixture	248:256	a mixture	248:256	The phytochemical composition and the antioxidant and anti-inflammatory activities of a mixture of 23 plants, named Horchata, traditionally consumed in Ecuador, have been evaluated.
31226431	6	5	theme	hydroalcoholic	1259:1272	arg1	medium					1274:1279	the hydroalcoholic medium	1255:1279	the hydroalcoholic medium	1255:1279	Greater protective effects were obtained for HHext compared to IHext, which was in agreement with its higher content of phenolic compounds favoured by a more efficient extraction in the hydroalcoholic medium.
31226431	4	6	theme	phenolic	714:721	arg1	compounds					723:731	phenolic compounds	714:731	phenolic compounds	714:731	Among phenolic compounds, caffeoylquinic acids, flavones and flavonols (mostly quercetin glycosides) were prominent.
31226431	5	7	theme	cytotoxic	916:924	arg1	damage					926:931	LPS-induced cytotoxic damage	904:931	LPS-induced cytotoxic damage	904:931	Both HHext and IHext extracts managed to protect RAW 264.7 macrophages against LPS-induced cytotoxic damage, increasing the levels of endogenous antioxidant enzymes and modulating the production of pro-inflammatory and anti-inflammatory cytokines.
31226431	0	8	theme	southern	82:89	arg1	Ecuador					91:97	southern Ecuador	82:97	southern Ecuador against LPS-induced cytotoxic damage in RAW 264.7 macrophages	82:159	Anti-inflammatory effect of the medicinal herbal mixture infusion, Horchata, from southern Ecuador against LPS-induced cytotoxic damage in RAW 264.7 macrophages.
31226431	3	9	theme	preparation	667:677	arg1	infusion					638:645	the infusion	634:645	the infusion	634:645	It was verified that thermal treatment affected the contents of vitamin C and carotenoids, but hardly those of polyphenols, which would be the main bioactive compounds in the infusion, the common form of preparation of horchata for consumption.
31226431	3	9	theme	preparation	667:677	arg1	form					659:662	the common form	648:662	the common form of preparation of horchata for consumption	648:705	It was verified that thermal treatment affected the contents of vitamin C and carotenoids, but hardly those of polyphenols, which would be the main bioactive compounds in the infusion, the common form of preparation of horchata for consumption.
31226431	6	10	from	extraction	1241:1250	arg1	medium					1274:1279	the hydroalcoholic medium	1255:1279	the hydroalcoholic medium	1255:1279	Greater protective effects were obtained for HHext compared to IHext, which was in agreement with its higher content of phenolic compounds favoured by a more efficient extraction in the hydroalcoholic medium.
31226431	0	11	theme	LPS-induced	107:117	arg1	damage					129:134	LPS-induced cytotoxic damage	107:134	LPS-induced cytotoxic damage in RAW 264.7 macrophages	107:159	Anti-inflammatory effect of the medicinal herbal mixture infusion, Horchata, from southern Ecuador against LPS-induced cytotoxic damage in RAW 264.7 macrophages.
31226431	6	12	theme	Greater	1073:1079	arg1	effects					1092:1098	Greater protective effects	1073:1098	Greater protective effects	1073:1098	Greater protective effects were obtained for HHext compared to IHext, which was in agreement with its higher content of phenolic compounds favoured by a more efficient extraction in the hydroalcoholic medium.
31226431	1	13	theme	phytochemical	166:178	arg1	composition					180:190	The phytochemical composition	162:190	The phytochemical composition	162:190	The phytochemical composition and the antioxidant and anti-inflammatory activities of a mixture of 23 plants, named Horchata, traditionally consumed in Ecuador, have been evaluated.
31226431	0	14	theme	herbal	42:47	arg1	Horchata					67:74	Horchata	67:74	Horchata	67:74	Anti-inflammatory effect of the medicinal herbal mixture infusion, Horchata, from southern Ecuador against LPS-induced cytotoxic damage in RAW 264.7 macrophages.
31226431	0	14	theme	herbal	42:47	arg1	infusion					57:64	the medicinal herbal mixture infusion	28:64	the medicinal herbal mixture infusion	28:64	Anti-inflammatory effect of the medicinal herbal mixture infusion, Horchata, from southern Ecuador against LPS-induced cytotoxic damage in RAW 264.7 macrophages.
31226431	2	15	theme	hydroalcoholic	380:393	arg1	HHext					404:408	HHext	404:408	HHext	404:408	The study was carried out using the hydroalcoholic extract (HHext) and infusion (IHext) of the horchata plant mixture.
31226431	2	15	theme	hydroalcoholic	380:393	arg1	extract					395:401	the hydroalcoholic extract	376:401	the hydroalcoholic extract (HHext)	376:409	The study was carried out using the hydroalcoholic extract (HHext) and infusion (IHext) of the horchata plant mixture.
31226431	6	16	theme	efficient	1231:1239	arg1	extraction					1241:1250	a more efficient extraction	1224:1250	a more efficient extraction in the hydroalcoholic medium	1224:1279	Greater protective effects were obtained for HHext compared to IHext, which was in agreement with its higher content of phenolic compounds favoured by a more efficient extraction in the hydroalcoholic medium.
31226431	4	17	dep	acids	749:753	arg1	glycosides					797:806	quercetin glycosides	787:806	mostly quercetin glycosides	780:806	Among phenolic compounds, caffeoylquinic acids, flavones and flavonols (mostly quercetin glycosides) were prominent.
31226431	0	18	theme	Anti-inflammatory	0:16	arg1	effect					18:23	Anti-inflammatory effect	0:23	Anti-inflammatory effect of the medicinal herbal mixture infusion, Horchata, from southern Ecuador against LPS-induced cytotoxic damage in RAW 264.7 macrophages.	0:160	Anti-inflammatory effect of the medicinal herbal mixture infusion, Horchata, from southern Ecuador against LPS-induced cytotoxic damage in RAW 264.7 macrophages.
31226431	6	19	with	agreement	1156:1164	arg1	content					1182:1188	its higher content	1171:1188	its higher content of phenolic compounds favoured by a more efficient extraction in the hydroalcoholic medium	1171:1279	Greater protective effects were obtained for HHext compared to IHext, which was in agreement with its higher content of phenolic compounds favoured by a more efficient extraction in the hydroalcoholic medium.
31226431	3	20	theme	carotenoids	541:551	arg1	contents					515:522	the contents	511:522	the contents of vitamin C and carotenoids	511:551	It was verified that thermal treatment affected the contents of vitamin C and carotenoids, but hardly those of polyphenols, which would be the main bioactive compounds in the infusion, the common form of preparation of horchata for consumption.
31226431	5	21	theme	cytokines	1062:1070	arg1	production					1009:1018	the production	1005:1018	the production of pro-inflammatory and anti-inflammatory cytokines	1005:1070	Both HHext and IHext extracts managed to protect RAW 264.7 macrophages against LPS-induced cytotoxic damage, increasing the levels of endogenous antioxidant enzymes and modulating the production of pro-inflammatory and anti-inflammatory cytokines.
31226431	7	22	theme	antioxidant	1339:1349	arg1	activity					1373:1380	a significant antioxidant and anti-inflammatory activity	1325:1380	a significant antioxidant and anti-inflammatory activity	1325:1380	Nonetheless, the infusion still maintained a significant antioxidant and anti-inflammatory activity, which would support the protective effects on health traditionally attributed to its consumption by the population.
31226431	6	23	theme	compounds	1202:1210	arg1	content					1182:1188	its higher content	1171:1188	its higher content of phenolic compounds favoured by a more efficient extraction in the hydroalcoholic medium	1171:1279	Greater protective effects were obtained for HHext compared to IHext, which was in agreement with its higher content of phenolic compounds favoured by a more efficient extraction in the hydroalcoholic medium.
31226431	3	24	theme	vitamin	527:533	arg1	C					535:535	vitamin C	527:535	vitamin C	527:535	It was verified that thermal treatment affected the contents of vitamin C and carotenoids, but hardly those of polyphenols, which would be the main bioactive compounds in the infusion, the common form of preparation of horchata for consumption.
31226431	1	25	theme	antioxidant	200:210	arg1	activities					234:243	the antioxidant and anti-inflammatory activities	196:243	the antioxidant and anti-inflammatory activities of a mixture of 23 plants, named Horchata, traditionally consumed in Ecuador,	196:321	The phytochemical composition and the antioxidant and anti-inflammatory activities of a mixture of 23 plants, named Horchata, traditionally consumed in Ecuador, have been evaluated.
31226431	3	26	theme	horchata	682:689	arg1	preparation					667:677	preparation	667:677	preparation of horchata for consumption	667:705	It was verified that thermal treatment affected the contents of vitamin C and carotenoids, but hardly those of polyphenols, which would be the main bioactive compounds in the infusion, the common form of preparation of horchata for consumption.
31226431	0	27	theme	cytotoxic	119:127	arg1	damage					129:134	LPS-induced cytotoxic damage	107:134	LPS-induced cytotoxic damage in RAW 264.7 macrophages	107:159	Anti-inflammatory effect of the medicinal herbal mixture infusion, Horchata, from southern Ecuador against LPS-induced cytotoxic damage in RAW 264.7 macrophages.
31226431	5	28	theme	anti-inflammatory	1044:1060	arg1	cytokines					1062:1070	pro-inflammatory and anti-inflammatory cytokines	1023:1070	pro-inflammatory and anti-inflammatory cytokines	1023:1070	Both HHext and IHext extracts managed to protect RAW 264.7 macrophages against LPS-induced cytotoxic damage, increasing the levels of endogenous antioxidant enzymes and modulating the production of pro-inflammatory and anti-inflammatory cytokines.
31226431	3	29	theme	C	535:535	arg1	contents					515:522	the contents	511:522	the contents of vitamin C and carotenoids	511:551	It was verified that thermal treatment affected the contents of vitamin C and carotenoids, but hardly those of polyphenols, which would be the main bioactive compounds in the infusion, the common form of preparation of horchata for consumption.
31226431	2	30	theme	plant	448:452	arg1	mixture					454:460	the horchata plant mixture	435:460	the horchata plant mixture	435:460	The study was carried out using the hydroalcoholic extract (HHext) and infusion (IHext) of the horchata plant mixture.
31226431	0	31	theme	RAW	139:141	arg1	macrophages					149:159	RAW 264.7 macrophages	139:159	RAW 264.7 macrophages	139:159	Anti-inflammatory effect of the medicinal herbal mixture infusion, Horchata, from southern Ecuador against LPS-induced cytotoxic damage in RAW 264.7 macrophages.
31226431	3	32	theme	main	606:609	arg1	compounds					621:629	the main bioactive compounds	602:629	the main bioactive compounds	602:629	It was verified that thermal treatment affected the contents of vitamin C and carotenoids, but hardly those of polyphenols, which would be the main bioactive compounds in the infusion, the common form of preparation of horchata for consumption.
31226431	3	32	theme	main	606:609	arg1	polyphenols					574:584	polyphenols	574:584	polyphenols	574:584	It was verified that thermal treatment affected the contents of vitamin C and carotenoids, but hardly those of polyphenols, which would be the main bioactive compounds in the infusion, the common form of preparation of horchata for consumption.
31226431	5	33	theme	enzymes	982:988	arg1	levels					949:954	the levels	945:954	the levels of endogenous antioxidant enzymes	945:988	Both HHext and IHext extracts managed to protect RAW 264.7 macrophages against LPS-induced cytotoxic damage, increasing the levels of endogenous antioxidant enzymes and modulating the production of pro-inflammatory and anti-inflammatory cytokines.
31226431	5	34	theme	HHext	830:834	arg1	extracts					846:853	Both HHext and IHext extracts	825:853	Both HHext and IHext extracts	825:853	Both HHext and IHext extracts managed to protect RAW 264.7 macrophages against LPS-induced cytotoxic damage, increasing the levels of endogenous antioxidant enzymes and modulating the production of pro-inflammatory and anti-inflammatory cytokines.
31226431	1	35	theme	anti-inflammatory	216:232	arg1	activities					234:243	the antioxidant and anti-inflammatory activities	196:243	the antioxidant and anti-inflammatory activities of a mixture of 23 plants, named Horchata, traditionally consumed in Ecuador,	196:321	The phytochemical composition and the antioxidant and anti-inflammatory activities of a mixture of 23 plants, named Horchata, traditionally consumed in Ecuador, have been evaluated.
31226431	2	36	theme	horchata	439:446	arg1	mixture					454:460	the horchata plant mixture	435:460	the horchata plant mixture	435:460	The study was carried out using the hydroalcoholic extract (HHext) and infusion (IHext) of the horchata plant mixture.
31226431	0	37	theme	medicinal	32:40	arg1	Horchata					67:74	Horchata	67:74	Horchata	67:74	Anti-inflammatory effect of the medicinal herbal mixture infusion, Horchata, from southern Ecuador against LPS-induced cytotoxic damage in RAW 264.7 macrophages.
31226431	0	37	theme	medicinal	32:40	arg1	infusion					57:64	the medicinal herbal mixture infusion	28:64	the medicinal herbal mixture infusion	28:64	Anti-inflammatory effect of the medicinal herbal mixture infusion, Horchata, from southern Ecuador against LPS-induced cytotoxic damage in RAW 264.7 macrophages.
31226431	0	38	from	damage	129:134	arg1	macrophages					149:159	RAW 264.7 macrophages	139:159	RAW 264.7 macrophages	139:159	Anti-inflammatory effect of the medicinal herbal mixture infusion, Horchata, from southern Ecuador against LPS-induced cytotoxic damage in RAW 264.7 macrophages.
31226431	0	39	theme	infusion	57:64	arg1	effect					18:23	Anti-inflammatory effect	0:23	Anti-inflammatory effect of the medicinal herbal mixture infusion, Horchata, from southern Ecuador against LPS-induced cytotoxic damage in RAW 264.7 macrophages.	0:160	Anti-inflammatory effect of the medicinal herbal mixture infusion, Horchata, from southern Ecuador against LPS-induced cytotoxic damage in RAW 264.7 macrophages.
31226431	0	40	theme	mixture	49:55	arg1	Horchata					67:74	Horchata	67:74	Horchata	67:74	Anti-inflammatory effect of the medicinal herbal mixture infusion, Horchata, from southern Ecuador against LPS-induced cytotoxic damage in RAW 264.7 macrophages.
31226431	0	40	theme	mixture	49:55	arg1	infusion					57:64	the medicinal herbal mixture infusion	28:64	the medicinal herbal mixture infusion	28:64	Anti-inflammatory effect of the medicinal herbal mixture infusion, Horchata, from southern Ecuador against LPS-induced cytotoxic damage in RAW 264.7 macrophages.
31226431	4	41	theme	quercetin	787:795	arg1	glycosides					797:806	quercetin glycosides	787:806	mostly quercetin glycosides	780:806	Among phenolic compounds, caffeoylquinic acids, flavones and flavonols (mostly quercetin glycosides) were prominent.
31226431	6	42	theme	higher	1175:1180	arg1	content					1182:1188	its higher content	1171:1188	its higher content of phenolic compounds favoured by a more efficient extraction in the hydroalcoholic medium	1171:1279	Greater protective effects were obtained for HHext compared to IHext, which was in agreement with its higher content of phenolic compounds favoured by a more efficient extraction in the hydroalcoholic medium.
31226431	7	43	theme	anti-inflammatory	1355:1371	arg1	activity					1373:1380	a significant antioxidant and anti-inflammatory activity	1325:1380	a significant antioxidant and anti-inflammatory activity	1325:1380	Nonetheless, the infusion still maintained a significant antioxidant and anti-inflammatory activity, which would support the protective effects on health traditionally attributed to its consumption by the population.
31226431	6	44	theme	phenolic	1193:1200	arg1	compounds					1202:1210	phenolic compounds	1193:1210	phenolic compounds favoured by a more efficient extraction in the hydroalcoholic medium	1193:1279	Greater protective effects were obtained for HHext compared to IHext, which was in agreement with its higher content of phenolic compounds favoured by a more efficient extraction in the hydroalcoholic medium.
31226431	7	45	dep	maintained	1314:1323	arg1	support					1395:1401	support	1395:1401	would support the protective effects on health traditionally attributed to its consumption by the population	1389:1496	Nonetheless, the infusion still maintained a significant antioxidant and anti-inflammatory activity, which would support the protective effects on health traditionally attributed to its consumption by the population.
31226431	5	46	theme	RAW	874:876	arg1	macrophages					884:894	RAW 264.7 macrophages	874:894	RAW 264.7 macrophages	874:894	Both HHext and IHext extracts managed to protect RAW 264.7 macrophages against LPS-induced cytotoxic damage, increasing the levels of endogenous antioxidant enzymes and modulating the production of pro-inflammatory and anti-inflammatory cytokines.
31226431	5	47	theme	LPS-induced	904:914	arg1	damage					926:931	LPS-induced cytotoxic damage	904:931	LPS-induced cytotoxic damage	904:931	Both HHext and IHext extracts managed to protect RAW 264.7 macrophages against LPS-induced cytotoxic damage, increasing the levels of endogenous antioxidant enzymes and modulating the production of pro-inflammatory and anti-inflammatory cytokines.
31226431	7	48	theme	protective	1407:1416	arg1	effects					1418:1424	the protective effects	1403:1424	the protective effects on health traditionally attributed to its consumption by the population	1403:1496	Nonetheless, the infusion still maintained a significant antioxidant and anti-inflammatory activity, which would support the protective effects on health traditionally attributed to its consumption by the population.
31226431	0	49	from	Ecuador	91:97	arg1	effect					18:23	Anti-inflammatory effect	0:23	Anti-inflammatory effect of the medicinal herbal mixture infusion, Horchata, from southern Ecuador against LPS-induced cytotoxic damage in RAW 264.7 macrophages.	0:160	Anti-inflammatory effect of the medicinal herbal mixture infusion, Horchata, from southern Ecuador against LPS-induced cytotoxic damage in RAW 264.7 macrophages.
31226431	4	50	theme	caffeoylquinic	734:747	arg1	acids					749:753	caffeoylquinic acids	734:753	caffeoylquinic acids	734:753	Among phenolic compounds, caffeoylquinic acids, flavones and flavonols (mostly quercetin glycosides) were prominent.
31226431	7	51	from	effects	1418:1424	arg1	health					1429:1434	health	1429:1434	health traditionally attributed to its consumption by the population	1429:1496	Nonetheless, the infusion still maintained a significant antioxidant and anti-inflammatory activity, which would support the protective effects on health traditionally attributed to its consumption by the population.
31226431	5	52	theme	pro-inflammatory	1023:1038	arg1	cytokines					1062:1070	pro-inflammatory and anti-inflammatory cytokines	1023:1070	pro-inflammatory and anti-inflammatory cytokines	1023:1070	Both HHext and IHext extracts managed to protect RAW 264.7 macrophages against LPS-induced cytotoxic damage, increasing the levels of endogenous antioxidant enzymes and modulating the production of pro-inflammatory and anti-inflammatory cytokines.
31226431	5	53	theme	endogenous	959:968	arg1	enzymes					982:988	endogenous antioxidant enzymes	959:988	endogenous antioxidant enzymes	959:988	Both HHext and IHext extracts managed to protect RAW 264.7 macrophages against LPS-induced cytotoxic damage, increasing the levels of endogenous antioxidant enzymes and modulating the production of pro-inflammatory and anti-inflammatory cytokines.
31226431	3	54	theme	thermal	484:490	arg1	treatment					492:500	thermal treatment	484:500	thermal treatment	484:500	It was verified that thermal treatment affected the contents of vitamin C and carotenoids, but hardly those of polyphenols, which would be the main bioactive compounds in the infusion, the common form of preparation of horchata for consumption.
31226431	7	55	theme	significant	1327:1337	arg1	activity					1373:1380	a significant antioxidant and anti-inflammatory activity	1325:1380	a significant antioxidant and anti-inflammatory activity	1325:1380	Nonetheless, the infusion still maintained a significant antioxidant and anti-inflammatory activity, which would support the protective effects on health traditionally attributed to its consumption by the population.
31226431	1	56	theme	mixture	250:256	arg1	composition					180:190	The phytochemical composition	162:190	The phytochemical composition	162:190	The phytochemical composition and the antioxidant and anti-inflammatory activities of a mixture of 23 plants, named Horchata, traditionally consumed in Ecuador, have been evaluated.
31226431	1	56	theme	mixture	250:256	arg1	activities					234:243	the antioxidant and anti-inflammatory activities	196:243	the antioxidant and anti-inflammatory activities of a mixture of 23 plants, named Horchata, traditionally consumed in Ecuador,	196:321	The phytochemical composition and the antioxidant and anti-inflammatory activities of a mixture of 23 plants, named Horchata, traditionally consumed in Ecuador, have been evaluated.
31226431	3	57	from	compounds	621:629	arg1	infusion					638:645	the infusion	634:645	the infusion	634:645	It was verified that thermal treatment affected the contents of vitamin C and carotenoids, but hardly those of polyphenols, which would be the main bioactive compounds in the infusion, the common form of preparation of horchata for consumption.
31226431	3	57	from	compounds	621:629	arg1	form					659:662	the common form	648:662	the common form of preparation of horchata for consumption	648:705	It was verified that thermal treatment affected the contents of vitamin C and carotenoids, but hardly those of polyphenols, which would be the main bioactive compounds in the infusion, the common form of preparation of horchata for consumption.
31226431	5	58	theme	antioxidant	970:980	arg1	enzymes					982:988	endogenous antioxidant enzymes	959:988	endogenous antioxidant enzymes	959:988	Both HHext and IHext extracts managed to protect RAW 264.7 macrophages against LPS-induced cytotoxic damage, increasing the levels of endogenous antioxidant enzymes and modulating the production of pro-inflammatory and anti-inflammatory cytokines.
31226431	5	59	theme	IHext	840:844	arg1	extracts					846:853	Both HHext and IHext extracts	825:853	Both HHext and IHext extracts	825:853	Both HHext and IHext extracts managed to protect RAW 264.7 macrophages against LPS-induced cytotoxic damage, increasing the levels of endogenous antioxidant enzymes and modulating the production of pro-inflammatory and anti-inflammatory cytokines.
29465340	7	0	theme	meso-diaminopimelic	1043:1061	arg1	type					1016:1019	The peptidoglycan type	998:1019	The peptidoglycan type of strain T3-209T	998:1037	The peptidoglycan type of strain T3-209T was meso-diaminopimelic acid.
29465340	7	0	theme	meso-diaminopimelic	1043:1061	arg1	acid					1063:1066	meso-diaminopimelic acid	1043:1066	meso-diaminopimelic acid	1043:1066	The peptidoglycan type of strain T3-209T was meso-diaminopimelic acid.
29465340	6	1	theme	species	989:995	arg1	strains					963:969	the type strains	954:969	the type strains of other Bacillus species	954:995	The 16S rRNA gene sequence analysis indicated that strain T3-209T belonged to the genus Bacillus within the family Bacillaceae and was most closely related to Bacillus drentensis LMG 21831T and Bacillus bataviensis LMG 21833T, with the same sequence similarity (97.0 %), but shared relatively low levels of similarity (92.6-96.9 %) to the type strains of other Bacillus species.
29465340	11	2	theme	41.8 mol	1362:1369	arg1	G+C content					1346:1356	The DNA G+C content	1338:1356	The DNA G+C content	1338:1356	The DNA G+C content was 41.8 mol%.
29465340	11	2	theme	41.8 mol	1362:1369	arg1	%					1370:1370	41.8 mol%	1362:1370	41.8 mol%	1362:1370	The DNA G+C content was 41.8 mol%.
29465340	6	3	theme	other	974:978	arg1	species					989:995	other Bacillus species	974:995	other Bacillus species	974:995	The 16S rRNA gene sequence analysis indicated that strain T3-209T belonged to the genus Bacillus within the family Bacillaceae and was most closely related to Bacillus drentensis LMG 21831T and Bacillus bataviensis LMG 21833T, with the same sequence similarity (97.0 %), but shared relatively low levels of similarity (92.6-96.9 %) to the type strains of other Bacillus species.
29465340	4	4	theme	%	551:551	arg1	w/v					539:541	w/v	539:541	w/v	539:541	Growth was observed at NaCl concentrations (w/v) of 0-6 % (optimum 0 %).
29465340	4	4	theme	%	551:551	arg1	concentrations					523:536	NaCl concentrations	518:536	NaCl concentrations (w/v) of 0-6 % (optimum 0 %)	518:565	Growth was observed at NaCl concentrations (w/v) of 0-6 % (optimum 0 %).
29465340	6	5	theme	gene	632:635	arg1	analysis					646:653	The 16S rRNA gene sequence analysis	619:653	The 16S rRNA gene sequence analysis	619:653	The 16S rRNA gene sequence analysis indicated that strain T3-209T belonged to the genus Bacillus within the family Bacillaceae and was most closely related to Bacillus drentensis LMG 21831T and Bacillus bataviensis LMG 21833T, with the same sequence similarity (97.0 %), but shared relatively low levels of similarity (92.6-96.9 %) to the type strains of other Bacillus species.
29465340	3	6	theme	temperature	448:458	arg1	range					460:464	a temperature range	446:464	a temperature range of 10-42 °C (optimum 28 °C)	446:492	The strain was able to grow at a temperature range of 10-42 °C (optimum 28 °C).
29465340	4	7	theme	optimum	554:560	arg1	%					551:551	0-6 %	547:551	0-6 % (optimum 0 %)	547:565	Growth was observed at NaCl concentrations (w/v) of 0-6 % (optimum 0 %).
29465340	4	7	theme	optimum	554:560	arg1	%					564:564	optimum 0 %	554:564	optimum 0 %	554:564	Growth was observed at NaCl concentrations (w/v) of 0-6 % (optimum 0 %).
29465340	6	8	theme	16S	623:625	arg1	rRNA					627:630	The 16S rRNA	619:630	The 16S rRNA gene sequence analysis	619:653	The 16S rRNA gene sequence analysis indicated that strain T3-209T belonged to the genus Bacillus within the family Bacillaceae and was most closely related to Bacillus drentensis LMG 21831T and Bacillus bataviensis LMG 21833T, with the same sequence similarity (97.0 %), but shared relatively low levels of similarity (92.6-96.9 %) to the type strains of other Bacillus species.
29465340	2	9	theme	Trough	286:291	arg1	1206 m					255:260	1206 m	255:260	1206 m of the southern Okinawa Trough at station T1 (25.07° N, 122.58° E) near the Tangyin hydrothermal vent during an expedition on the R/V Kexue in May 2014	255:412	A Gram-stain-positive, endospore-forming, rod-shaped, motile and strictly aerobic strain, designated T3-209T, was isolated from sediment sampled at a water depth of 1206 m of the southern Okinawa Trough at station T1 (25.07° N, 122.58° E) near the Tangyin hydrothermal vent during an expedition on the R/V Kexue in May 2014.
29465340	6	10	theme	Bacillus	813:820	arg1	21833T					838:843	Bacillus bataviensis LMG 21833T	813:843	Bacillus bataviensis LMG 21833T	813:843	The 16S rRNA gene sequence analysis indicated that strain T3-209T belonged to the genus Bacillus within the family Bacillaceae and was most closely related to Bacillus drentensis LMG 21831T and Bacillus bataviensis LMG 21833T, with the same sequence similarity (97.0 %), but shared relatively low levels of similarity (92.6-96.9 %) to the type strains of other Bacillus species.
29465340	8	11	theme	cellular	1085:1092	arg1	anteiso-C15 					1111:1122	anteiso-C15 	1111:1122	anteiso-C15 	1111:1122	The predominant cellular fatty acids were anteiso-C15 : 0, iso-C15 : 0 and alcohol-C16 : 1ω7c.
29465340	8	11	theme	cellular	1085:1092	arg1	acids					1100:1104	The predominant cellular fatty acids	1069:1104	The predominant cellular fatty acids	1069:1104	The predominant cellular fatty acids were anteiso-C15 : 0, iso-C15 : 0 and alcohol-C16 : 1ω7c.
29465340	5	12	theme	pH	572:573	arg1	7.0-9.0					596:602	7.0-9.0	596:602	7.0-9.0	596:602	The pH range for growth was 7.0-9.0 (optimum 8.0).
29465340	5	12	theme	pH	572:573	arg1	range					575:579	The pH range	568:579	The pH range for growth	568:590	The pH range for growth was 7.0-9.0 (optimum 8.0).
29465340	2	13	theme	southern	269:276	arg1	Trough					286:291	the southern Okinawa Trough	265:291	the southern Okinawa Trough	265:291	A Gram-stain-positive, endospore-forming, rod-shaped, motile and strictly aerobic strain, designated T3-209T, was isolated from sediment sampled at a water depth of 1206 m of the southern Okinawa Trough at station T1 (25.07° N, 122.58° E) near the Tangyin hydrothermal vent during an expedition on the R/V Kexue in May 2014.
29465340	12	14	theme	characterization	1428:1443	arg1	results					1383:1389	results	1383:1389	results of phylogenetic analysis, phenotypic characterization and chemotaxonomic studies	1383:1470	Combining results of phylogenetic analysis, phenotypic characterization and chemotaxonomic studies, strain T3-209T represents a novel species of the genus Bacillus, for which the name Bacillus alkalitolerans sp.
29465340	2	15	theme	water	240:244	arg1	depth					246:250	a water depth	238:250	a water depth of 1206 m of the southern Okinawa Trough at station T1 (25.07° N, 122.58° E) near the Tangyin hydrothermal vent during an expedition on the R/V Kexue in May 2014	238:412	A Gram-stain-positive, endospore-forming, rod-shaped, motile and strictly aerobic strain, designated T3-209T, was isolated from sediment sampled at a water depth of 1206 m of the southern Okinawa Trough at station T1 (25.07° N, 122.58° E) near the Tangyin hydrothermal vent during an expedition on the R/V Kexue in May 2014.
29465340	12	16	theme	alkalitolerans	1566:1579	arg1	sp					1581:1582	Bacillus alkalitolerans sp	1557:1582	the name Bacillus alkalitolerans sp	1548:1582	Combining results of phylogenetic analysis, phenotypic characterization and chemotaxonomic studies, strain T3-209T represents a novel species of the genus Bacillus, for which the name Bacillus alkalitolerans sp.
29465340	6	17	theme	sequence	860:867	arg1	%					886:886	97.0 %	881:886	97.0 %	881:886	The 16S rRNA gene sequence analysis indicated that strain T3-209T belonged to the genus Bacillus within the family Bacillaceae and was most closely related to Bacillus drentensis LMG 21831T and Bacillus bataviensis LMG 21833T, with the same sequence similarity (97.0 %), but shared relatively low levels of similarity (92.6-96.9 %) to the type strains of other Bacillus species.
29465340	6	17	theme	sequence	860:867	arg1	similarity					869:878	the same sequence similarity	851:878	the same sequence similarity (97.0 %)	851:887	The 16S rRNA gene sequence analysis indicated that strain T3-209T belonged to the genus Bacillus within the family Bacillaceae and was most closely related to Bacillus drentensis LMG 21831T and Bacillus bataviensis LMG 21833T, with the same sequence similarity (97.0 %), but shared relatively low levels of similarity (92.6-96.9 %) to the type strains of other Bacillus species.
29465340	6	18	dep	Bacillus	813:820	arg1	bataviensis					822:832	bataviensis	822:832	bataviensis	822:832	The 16S rRNA gene sequence analysis indicated that strain T3-209T belonged to the genus Bacillus within the family Bacillaceae and was most closely related to Bacillus drentensis LMG 21831T and Bacillus bataviensis LMG 21833T, with the same sequence similarity (97.0 %), but shared relatively low levels of similarity (92.6-96.9 %) to the type strains of other Bacillus species.
29465340	12	19	theme	phylogenetic	1394:1405	arg1	analysis					1407:1414	phylogenetic analysis	1394:1414	phylogenetic analysis	1394:1414	Combining results of phylogenetic analysis, phenotypic characterization and chemotaxonomic studies, strain T3-209T represents a novel species of the genus Bacillus, for which the name Bacillus alkalitolerans sp.
29465340	2	20	from	expedition	374:383	arg1	Kexue					396:400	the R/V Kexue	388:400	the R/V Kexue in May 2014	388:412	A Gram-stain-positive, endospore-forming, rod-shaped, motile and strictly aerobic strain, designated T3-209T, was isolated from sediment sampled at a water depth of 1206 m of the southern Okinawa Trough at station T1 (25.07° N, 122.58° E) near the Tangyin hydrothermal vent during an expedition on the R/V Kexue in May 2014.
29465340	7	21	theme	strain	1024:1029	arg1	T3-209T					1031:1037	strain T3-209T	1024:1037	strain T3-209T	1024:1037	The peptidoglycan type of strain T3-209T was meso-diaminopimelic acid.
29465340	11	22	theme	DNA	1342:1344	arg1	G+C content					1346:1356	The DNA G+C content	1338:1356	The DNA G+C content	1338:1356	The DNA G+C content was 41.8 mol%.
29465340	11	22	theme	DNA	1342:1344	arg1	%					1370:1370	41.8 mol%	1362:1370	41.8 mol%	1362:1370	The DNA G+C content was 41.8 mol%.
29465340	2	23	from	depth	246:250	arg1	T1					304:305	station T1	296:305	station T1 (25.07° N, 122.58° E) near the Tangyin hydrothermal vent during an expedition on the R/V Kexue in May 2014	296:412	A Gram-stain-positive, endospore-forming, rod-shaped, motile and strictly aerobic strain, designated T3-209T, was isolated from sediment sampled at a water depth of 1206 m of the southern Okinawa Trough at station T1 (25.07° N, 122.58° E) near the Tangyin hydrothermal vent during an expedition on the R/V Kexue in May 2014.
29465340	2	23	from	depth	246:250	arg1	N					315:315	25.07° N	308:315	25.07° N	308:315	A Gram-stain-positive, endospore-forming, rod-shaped, motile and strictly aerobic strain, designated T3-209T, was isolated from sediment sampled at a water depth of 1206 m of the southern Okinawa Trough at station T1 (25.07° N, 122.58° E) near the Tangyin hydrothermal vent during an expedition on the R/V Kexue in May 2014.
29465340	6	24	theme	LMG	834:836	arg1	21833T					838:843	Bacillus bataviensis LMG 21833T	813:843	Bacillus bataviensis LMG 21833T	813:843	The 16S rRNA gene sequence analysis indicated that strain T3-209T belonged to the genus Bacillus within the family Bacillaceae and was most closely related to Bacillus drentensis LMG 21831T and Bacillus bataviensis LMG 21833T, with the same sequence similarity (97.0 %), but shared relatively low levels of similarity (92.6-96.9 %) to the type strains of other Bacillus species.
29465340	2	25	from	Kexue	396:400	arg1	May					405:407	May 2014	405:412	May 2014	405:412	A Gram-stain-positive, endospore-forming, rod-shaped, motile and strictly aerobic strain, designated T3-209T, was isolated from sediment sampled at a water depth of 1206 m of the southern Okinawa Trough at station T1 (25.07° N, 122.58° E) near the Tangyin hydrothermal vent during an expedition on the R/V Kexue in May 2014.
29465340	2	26	theme	Tangyin	338:344	arg1	vent					359:362	the Tangyin hydrothermal vent	334:362	the Tangyin hydrothermal vent	334:362	A Gram-stain-positive, endospore-forming, rod-shaped, motile and strictly aerobic strain, designated T3-209T, was isolated from sediment sampled at a water depth of 1206 m of the southern Okinawa Trough at station T1 (25.07° N, 122.58° E) near the Tangyin hydrothermal vent during an expedition on the R/V Kexue in May 2014.
29465340	2	27	theme	R/V	392:394	arg1	Kexue					396:400	the R/V Kexue	388:400	the R/V Kexue in May 2014	388:412	A Gram-stain-positive, endospore-forming, rod-shaped, motile and strictly aerobic strain, designated T3-209T, was isolated from sediment sampled at a water depth of 1206 m of the southern Okinawa Trough at station T1 (25.07° N, 122.58° E) near the Tangyin hydrothermal vent during an expedition on the R/V Kexue in May 2014.
29465340	10	28	theme	unidentified	1278:1289	arg1	phosphatidylglycerol					1252:1271	phosphatidylglycerol	1252:1271	phosphatidylglycerol	1252:1271	The polar lipids of strain T3-209T comprised phosphatidylglycerol, two unidentified phospholipids and one unidentified aminolipid.
29465340	10	28	theme	unidentified	1278:1289	arg1	phospholipids					1291:1303	two unidentified phospholipids	1274:1303	two unidentified phospholipids	1274:1303	The polar lipids of strain T3-209T comprised phosphatidylglycerol, two unidentified phospholipids and one unidentified aminolipid.
29465340	0	29	theme	Bacillus	0:7	arg1	sp					24:25	Bacillus alkalitolerans sp	0:25	Bacillus alkalitolerans sp.	0:26	Bacillus alkalitolerans sp.
29465340	14	30	theme	33631T=DSM	1637:1646	arg1	T3-209T					1622:1628	T3-209T	1622:1628	T3-209T (=KCTC 33631T=DSM 29135T=MCCC 1K00503T)	1622:1668	The type strain is T3-209T (=KCTC 33631T=DSM 29135T=MCCC 1K00503T).
29465340	14	30	theme	33631T=DSM	1637:1646	arg1	1K00503T					1660:1667	=KCTC 33631T=DSM 29135T=MCCC 1K00503T	1631:1667	=KCTC 33631T=DSM 29135T=MCCC 1K00503T	1631:1667	The type strain is T3-209T (=KCTC 33631T=DSM 29135T=MCCC 1K00503T).
29465340	12	31	theme	novel	1501:1505	arg1	species					1507:1513	a novel species	1499:1513	a novel species	1499:1513	Combining results of phylogenetic analysis, phenotypic characterization and chemotaxonomic studies, strain T3-209T represents a novel species of the genus Bacillus, for which the name Bacillus alkalitolerans sp.
29465340	10	32	theme	unidentified	1313:1324	arg1	aminolipid					1326:1335	one unidentified aminolipid	1309:1335	one unidentified aminolipid	1309:1335	The polar lipids of strain T3-209T comprised phosphatidylglycerol, two unidentified phospholipids and one unidentified aminolipid.
29465340	10	32	theme	unidentified	1313:1324	arg1	phosphatidylglycerol					1252:1271	phosphatidylglycerol	1252:1271	phosphatidylglycerol	1252:1271	The polar lipids of strain T3-209T comprised phosphatidylglycerol, two unidentified phospholipids and one unidentified aminolipid.
29465340	4	33	located	observed	506:513	arg2	Growth					495:500	Growth	495:500	Growth	495:500	Growth was observed at NaCl concentrations (w/v) of 0-6 % (optimum 0 %).
29465340	4	33	located	observed	506:513	arg1	w/v					539:541	w/v	539:541	w/v	539:541	Growth was observed at NaCl concentrations (w/v) of 0-6 % (optimum 0 %).
29465340	4	33	located	observed	506:513	arg1	concentrations					523:536	NaCl concentrations	518:536	NaCl concentrations (w/v) of 0-6 % (optimum 0 %)	518:565	Growth was observed at NaCl concentrations (w/v) of 0-6 % (optimum 0 %).
29465340	6	34	theme	same	855:858	arg1	%					886:886	97.0 %	881:886	97.0 %	881:886	The 16S rRNA gene sequence analysis indicated that strain T3-209T belonged to the genus Bacillus within the family Bacillaceae and was most closely related to Bacillus drentensis LMG 21831T and Bacillus bataviensis LMG 21833T, with the same sequence similarity (97.0 %), but shared relatively low levels of similarity (92.6-96.9 %) to the type strains of other Bacillus species.
29465340	6	34	theme	same	855:858	arg1	similarity					869:878	the same sequence similarity	851:878	the same sequence similarity (97.0 %)	851:887	The 16S rRNA gene sequence analysis indicated that strain T3-209T belonged to the genus Bacillus within the family Bacillaceae and was most closely related to Bacillus drentensis LMG 21831T and Bacillus bataviensis LMG 21833T, with the same sequence similarity (97.0 %), but shared relatively low levels of similarity (92.6-96.9 %) to the type strains of other Bacillus species.
29465340	9	35	theme	respiratory	1168:1178	arg1	menaquinone-7					1192:1204	menaquinone-7	1192:1204	menaquinone-7	1192:1204	The respiratory quinone was menaquinone-7.
29465340	9	35	theme	respiratory	1168:1178	arg1	quinone					1180:1186	The respiratory quinone	1164:1186	The respiratory quinone	1164:1186	The respiratory quinone was menaquinone-7.
29465340	10	36	theme	strain	1227:1232	arg1	T3-209T					1234:1240	strain T3-209T	1227:1240	strain T3-209T	1227:1240	The polar lipids of strain T3-209T comprised phosphatidylglycerol, two unidentified phospholipids and one unidentified aminolipid.
29465340	14	37	theme	type	1607:1610	arg1	T3-209T					1622:1628	T3-209T	1622:1628	T3-209T (=KCTC 33631T=DSM 29135T=MCCC 1K00503T)	1622:1668	The type strain is T3-209T (=KCTC 33631T=DSM 29135T=MCCC 1K00503T).
29465340	14	37	theme	type	1607:1610	arg1	strain					1612:1617	The type strain	1603:1617	The type strain	1603:1617	The type strain is T3-209T (=KCTC 33631T=DSM 29135T=MCCC 1K00503T).
29465340	4	38	theme	NaCl	518:521	arg1	w/v					539:541	w/v	539:541	w/v	539:541	Growth was observed at NaCl concentrations (w/v) of 0-6 % (optimum 0 %).
29465340	4	38	theme	NaCl	518:521	arg1	concentrations					523:536	NaCl concentrations	518:536	NaCl concentrations (w/v) of 0-6 % (optimum 0 %)	518:565	Growth was observed at NaCl concentrations (w/v) of 0-6 % (optimum 0 %).
29465340	2	39	theme	endospore-forming	113:129	arg1	strain					172:177	A Gram-stain-positive, endospore-forming, rod-shaped, motile and strictly aerobic strain	90:177	A Gram-stain-positive, endospore-forming, rod-shaped, motile and strictly aerobic strain	90:177	A Gram-stain-positive, endospore-forming, rod-shaped, motile and strictly aerobic strain, designated T3-209T, was isolated from sediment sampled at a water depth of 1206 m of the southern Okinawa Trough at station T1 (25.07° N, 122.58° E) near the Tangyin hydrothermal vent during an expedition on the R/V Kexue in May 2014.
29465340	2	40	from	T1	304:305	arg1	1206 m					255:260	1206 m	255:260	1206 m of the southern Okinawa Trough at station T1 (25.07° N, 122.58° E) near the Tangyin hydrothermal vent during an expedition on the R/V Kexue in May 2014	255:412	A Gram-stain-positive, endospore-forming, rod-shaped, motile and strictly aerobic strain, designated T3-209T, was isolated from sediment sampled at a water depth of 1206 m of the southern Okinawa Trough at station T1 (25.07° N, 122.58° E) near the Tangyin hydrothermal vent during an expedition on the R/V Kexue in May 2014.
29465340	2	40	from	T1	304:305	arg1	depth					246:250	a water depth	238:250	a water depth of 1206 m of the southern Okinawa Trough at station T1 (25.07° N, 122.58° E) near the Tangyin hydrothermal vent during an expedition on the R/V Kexue in May 2014	238:412	A Gram-stain-positive, endospore-forming, rod-shaped, motile and strictly aerobic strain, designated T3-209T, was isolated from sediment sampled at a water depth of 1206 m of the southern Okinawa Trough at station T1 (25.07° N, 122.58° E) near the Tangyin hydrothermal vent during an expedition on the R/V Kexue in May 2014.
29465340	6	41	theme	low	912:914	arg1	levels					916:921	relatively low levels	901:921	relatively low levels of similarity (92.6-96.9 %) to the type strains of other Bacillus species	901:995	The 16S rRNA gene sequence analysis indicated that strain T3-209T belonged to the genus Bacillus within the family Bacillaceae and was most closely related to Bacillus drentensis LMG 21831T and Bacillus bataviensis LMG 21833T, with the same sequence similarity (97.0 %), but shared relatively low levels of similarity (92.6-96.9 %) to the type strains of other Bacillus species.
29465340	2	42	theme	Gram-stain-positive	92:110	arg1	strain					172:177	A Gram-stain-positive, endospore-forming, rod-shaped, motile and strictly aerobic strain	90:177	A Gram-stain-positive, endospore-forming, rod-shaped, motile and strictly aerobic strain	90:177	A Gram-stain-positive, endospore-forming, rod-shaped, motile and strictly aerobic strain, designated T3-209T, was isolated from sediment sampled at a water depth of 1206 m of the southern Okinawa Trough at station T1 (25.07° N, 122.58° E) near the Tangyin hydrothermal vent during an expedition on the R/V Kexue in May 2014.
29465340	6	43	theme	Bacillus	980:987	arg1	species					989:995	other Bacillus species	974:995	other Bacillus species	974:995	The 16S rRNA gene sequence analysis indicated that strain T3-209T belonged to the genus Bacillus within the family Bacillaceae and was most closely related to Bacillus drentensis LMG 21831T and Bacillus bataviensis LMG 21833T, with the same sequence similarity (97.0 %), but shared relatively low levels of similarity (92.6-96.9 %) to the type strains of other Bacillus species.
29465340	2	44	theme	motile	144:149	arg1	strain					172:177	A Gram-stain-positive, endospore-forming, rod-shaped, motile and strictly aerobic strain	90:177	A Gram-stain-positive, endospore-forming, rod-shaped, motile and strictly aerobic strain	90:177	A Gram-stain-positive, endospore-forming, rod-shaped, motile and strictly aerobic strain, designated T3-209T, was isolated from sediment sampled at a water depth of 1206 m of the southern Okinawa Trough at station T1 (25.07° N, 122.58° E) near the Tangyin hydrothermal vent during an expedition on the R/V Kexue in May 2014.
29465340	6	45	theme	sequence	637:644	arg1	analysis					646:653	The 16S rRNA gene sequence analysis	619:653	The 16S rRNA gene sequence analysis	619:653	The 16S rRNA gene sequence analysis indicated that strain T3-209T belonged to the genus Bacillus within the family Bacillaceae and was most closely related to Bacillus drentensis LMG 21831T and Bacillus bataviensis LMG 21833T, with the same sequence similarity (97.0 %), but shared relatively low levels of similarity (92.6-96.9 %) to the type strains of other Bacillus species.
29465340	2	46	theme	1206 m	255:260	arg1	depth					246:250	a water depth	238:250	a water depth of 1206 m of the southern Okinawa Trough at station T1 (25.07° N, 122.58° E) near the Tangyin hydrothermal vent during an expedition on the R/V Kexue in May 2014	238:412	A Gram-stain-positive, endospore-forming, rod-shaped, motile and strictly aerobic strain, designated T3-209T, was isolated from sediment sampled at a water depth of 1206 m of the southern Okinawa Trough at station T1 (25.07° N, 122.58° E) near the Tangyin hydrothermal vent during an expedition on the R/V Kexue in May 2014.
29465340	6	47	theme	family	727:732	arg1	Bacillaceae					734:744	the family Bacillaceae	723:744	the family Bacillaceae	723:744	The 16S rRNA gene sequence analysis indicated that strain T3-209T belonged to the genus Bacillus within the family Bacillaceae and was most closely related to Bacillus drentensis LMG 21831T and Bacillus bataviensis LMG 21833T, with the same sequence similarity (97.0 %), but shared relatively low levels of similarity (92.6-96.9 %) to the type strains of other Bacillus species.
29465340	4	48	theme	0-6 	547:550	arg1	%					551:551	0-6 %	547:551	0-6 % (optimum 0 %)	547:565	Growth was observed at NaCl concentrations (w/v) of 0-6 % (optimum 0 %).
29465340	4	48	theme	0-6 	547:550	arg1	%					564:564	optimum 0 %	554:564	optimum 0 %	554:564	Growth was observed at NaCl concentrations (w/v) of 0-6 % (optimum 0 %).
29465340	2	49	theme	rod-shaped	132:141	arg1	strain					172:177	A Gram-stain-positive, endospore-forming, rod-shaped, motile and strictly aerobic strain	90:177	A Gram-stain-positive, endospore-forming, rod-shaped, motile and strictly aerobic strain	90:177	A Gram-stain-positive, endospore-forming, rod-shaped, motile and strictly aerobic strain, designated T3-209T, was isolated from sediment sampled at a water depth of 1206 m of the southern Okinawa Trough at station T1 (25.07° N, 122.58° E) near the Tangyin hydrothermal vent during an expedition on the R/V Kexue in May 2014.
29465340	6	50	theme	rRNA	627:630	arg1	analysis					646:653	The 16S rRNA gene sequence analysis	619:653	The 16S rRNA gene sequence analysis	619:653	The 16S rRNA gene sequence analysis indicated that strain T3-209T belonged to the genus Bacillus within the family Bacillaceae and was most closely related to Bacillus drentensis LMG 21831T and Bacillus bataviensis LMG 21833T, with the same sequence similarity (97.0 %), but shared relatively low levels of similarity (92.6-96.9 %) to the type strains of other Bacillus species.
29465340	3	51	theme	optimum	479:485	arg1	10-42 °C					469:476	10-42 °C	469:476	10-42 °C (optimum 28 °C)	469:492	The strain was able to grow at a temperature range of 10-42 °C (optimum 28 °C).
29465340	3	51	theme	optimum	479:485	arg1	28 °C					487:491	optimum 28 °C	479:491	optimum 28 °C	479:491	The strain was able to grow at a temperature range of 10-42 °C (optimum 28 °C).
29465340	12	52	theme	studies	1464:1470	arg1	results					1383:1389	results	1383:1389	results of phylogenetic analysis, phenotypic characterization and chemotaxonomic studies	1383:1470	Combining results of phylogenetic analysis, phenotypic characterization and chemotaxonomic studies, strain T3-209T represents a novel species of the genus Bacillus, for which the name Bacillus alkalitolerans sp.
29465340	6	53	theme	type	958:961	arg1	strains					963:969	the type strains	954:969	the type strains of other Bacillus species	954:995	The 16S rRNA gene sequence analysis indicated that strain T3-209T belonged to the genus Bacillus within the family Bacillaceae and was most closely related to Bacillus drentensis LMG 21831T and Bacillus bataviensis LMG 21833T, with the same sequence similarity (97.0 %), but shared relatively low levels of similarity (92.6-96.9 %) to the type strains of other Bacillus species.
29465340	12	54	dep	name	1552:1555	arg1	sp					1581:1582	Bacillus alkalitolerans sp	1557:1582	the name Bacillus alkalitolerans sp	1548:1582	Combining results of phylogenetic analysis, phenotypic characterization and chemotaxonomic studies, strain T3-209T represents a novel species of the genus Bacillus, for which the name Bacillus alkalitolerans sp.
29465340	10	55	theme	polar	1211:1215	arg1	lipids					1217:1222	The polar lipids	1207:1222	The polar lipids of strain T3-209T	1207:1240	The polar lipids of strain T3-209T comprised phosphatidylglycerol, two unidentified phospholipids and one unidentified aminolipid.
29465340	2	56	theme	Okinawa	278:284	arg1	Trough					286:291	the southern Okinawa Trough	265:291	the southern Okinawa Trough	265:291	A Gram-stain-positive, endospore-forming, rod-shaped, motile and strictly aerobic strain, designated T3-209T, was isolated from sediment sampled at a water depth of 1206 m of the southern Okinawa Trough at station T1 (25.07° N, 122.58° E) near the Tangyin hydrothermal vent during an expedition on the R/V Kexue in May 2014.
29465340	12	57	theme	strain	1473:1478	arg1	T3-209T					1480:1486	strain T3-209T	1473:1486	strain T3-209T	1473:1486	Combining results of phylogenetic analysis, phenotypic characterization and chemotaxonomic studies, strain T3-209T represents a novel species of the genus Bacillus, for which the name Bacillus alkalitolerans sp.
29465340	6	58	dep	Bacillus	778:785	arg1	drentensis					787:796	drentensis	787:796	drentensis	787:796	The 16S rRNA gene sequence analysis indicated that strain T3-209T belonged to the genus Bacillus within the family Bacillaceae and was most closely related to Bacillus drentensis LMG 21831T and Bacillus bataviensis LMG 21833T, with the same sequence similarity (97.0 %), but shared relatively low levels of similarity (92.6-96.9 %) to the type strains of other Bacillus species.
29465340	8	59	theme	predominant	1073:1083	arg1	anteiso-C15 					1111:1122	anteiso-C15 	1111:1122	anteiso-C15 	1111:1122	The predominant cellular fatty acids were anteiso-C15 : 0, iso-C15 : 0 and alcohol-C16 : 1ω7c.
29465340	8	59	theme	predominant	1073:1083	arg1	acids					1100:1104	The predominant cellular fatty acids	1069:1104	The predominant cellular fatty acids	1069:1104	The predominant cellular fatty acids were anteiso-C15 : 0, iso-C15 : 0 and alcohol-C16 : 1ω7c.
29465340	6	60	theme	LMG	798:800	arg1	21831T					802:807	Bacillus drentensis LMG 21831T	778:807	Bacillus drentensis LMG 21831T	778:807	The 16S rRNA gene sequence analysis indicated that strain T3-209T belonged to the genus Bacillus within the family Bacillaceae and was most closely related to Bacillus drentensis LMG 21831T and Bacillus bataviensis LMG 21833T, with the same sequence similarity (97.0 %), but shared relatively low levels of similarity (92.6-96.9 %) to the type strains of other Bacillus species.
29465340	8	61	theme	fatty	1094:1098	arg1	anteiso-C15 					1111:1122	anteiso-C15 	1111:1122	anteiso-C15 	1111:1122	The predominant cellular fatty acids were anteiso-C15 : 0, iso-C15 : 0 and alcohol-C16 : 1ω7c.
29465340	8	61	theme	fatty	1094:1098	arg1	acids					1100:1104	The predominant cellular fatty acids	1069:1104	The predominant cellular fatty acids	1069:1104	The predominant cellular fatty acids were anteiso-C15 : 0, iso-C15 : 0 and alcohol-C16 : 1ω7c.
29465340	12	62	theme	chemotaxonomic	1449:1462	arg1	studies					1464:1470	chemotaxonomic studies	1449:1470	chemotaxonomic studies	1449:1470	Combining results of phylogenetic analysis, phenotypic characterization and chemotaxonomic studies, strain T3-209T represents a novel species of the genus Bacillus, for which the name Bacillus alkalitolerans sp.
29465340	6	63	theme	Bacillus	778:785	arg1	21831T					802:807	Bacillus drentensis LMG 21831T	778:807	Bacillus drentensis LMG 21831T	778:807	The 16S rRNA gene sequence analysis indicated that strain T3-209T belonged to the genus Bacillus within the family Bacillaceae and was most closely related to Bacillus drentensis LMG 21831T and Bacillus bataviensis LMG 21833T, with the same sequence similarity (97.0 %), but shared relatively low levels of similarity (92.6-96.9 %) to the type strains of other Bacillus species.
29465340	3	64	theme	10-42 °C	469:476	arg1	range					460:464	a temperature range	446:464	a temperature range of 10-42 °C (optimum 28 °C)	446:492	The strain was able to grow at a temperature range of 10-42 °C (optimum 28 °C).
29465340	7	65	theme	T3-209T	1031:1037	arg1	type					1016:1019	The peptidoglycan type	998:1019	The peptidoglycan type of strain T3-209T	998:1037	The peptidoglycan type of strain T3-209T was meso-diaminopimelic acid.
29465340	7	65	theme	T3-209T	1031:1037	arg1	acid					1063:1066	meso-diaminopimelic acid	1043:1066	meso-diaminopimelic acid	1043:1066	The peptidoglycan type of strain T3-209T was meso-diaminopimelic acid.
29465340	12	66	theme	Bacillus	1557:1564	arg1	sp					1581:1582	Bacillus alkalitolerans sp	1557:1582	the name Bacillus alkalitolerans sp	1548:1582	Combining results of phylogenetic analysis, phenotypic characterization and chemotaxonomic studies, strain T3-209T represents a novel species of the genus Bacillus, for which the name Bacillus alkalitolerans sp.
29465340	12	67	theme	analysis	1407:1414	arg1	results					1383:1389	results	1383:1389	results of phylogenetic analysis, phenotypic characterization and chemotaxonomic studies	1383:1470	Combining results of phylogenetic analysis, phenotypic characterization and chemotaxonomic studies, strain T3-209T represents a novel species of the genus Bacillus, for which the name Bacillus alkalitolerans sp.
29465340	4	68	theme	0 	562:563	arg1	%					551:551	0-6 %	547:551	0-6 % (optimum 0 %)	547:565	Growth was observed at NaCl concentrations (w/v) of 0-6 % (optimum 0 %).
29465340	4	68	theme	0 	562:563	arg1	%					564:564	optimum 0 %	554:564	optimum 0 %	554:564	Growth was observed at NaCl concentrations (w/v) of 0-6 % (optimum 0 %).
29465340	12	69	theme	Bacillus	1528:1535	arg1	species					1507:1513	a novel species	1499:1513	a novel species	1499:1513	Combining results of phylogenetic analysis, phenotypic characterization and chemotaxonomic studies, strain T3-209T represents a novel species of the genus Bacillus, for which the name Bacillus alkalitolerans sp.
29465340	7	70	theme	peptidoglycan	1002:1014	arg1	type					1016:1019	The peptidoglycan type	998:1019	The peptidoglycan type of strain T3-209T	998:1037	The peptidoglycan type of strain T3-209T was meso-diaminopimelic acid.
29465340	7	70	theme	peptidoglycan	1002:1014	arg1	acid					1063:1066	meso-diaminopimelic acid	1043:1066	meso-diaminopimelic acid	1043:1066	The peptidoglycan type of strain T3-209T was meso-diaminopimelic acid.
29465340	2	71	attach	isolated	204:211	arg1	sediment					218:225	sediment	218:225	sediment sampled at a water depth of 1206 m of the southern Okinawa Trough at station T1 (25.07° N, 122.58° E) near the Tangyin hydrothermal vent during an expedition on the R/V Kexue in May 2014	218:412	A Gram-stain-positive, endospore-forming, rod-shaped, motile and strictly aerobic strain, designated T3-209T, was isolated from sediment sampled at a water depth of 1206 m of the southern Okinawa Trough at station T1 (25.07° N, 122.58° E) near the Tangyin hydrothermal vent during an expedition on the R/V Kexue in May 2014.
29465340	2	71	attach	isolated	204:211	arg2	strain					172:177	A Gram-stain-positive, endospore-forming, rod-shaped, motile and strictly aerobic strain	90:177	A Gram-stain-positive, endospore-forming, rod-shaped, motile and strictly aerobic strain	90:177	A Gram-stain-positive, endospore-forming, rod-shaped, motile and strictly aerobic strain, designated T3-209T, was isolated from sediment sampled at a water depth of 1206 m of the southern Okinawa Trough at station T1 (25.07° N, 122.58° E) near the Tangyin hydrothermal vent during an expedition on the R/V Kexue in May 2014.
29465340	10	72	theme	T3-209T	1234:1240	arg1	lipids					1217:1222	The polar lipids	1207:1222	The polar lipids of strain T3-209T	1207:1240	The polar lipids of strain T3-209T comprised phosphatidylglycerol, two unidentified phospholipids and one unidentified aminolipid.
29465340	2	73	dep	N	315:315	arg1	E					326:326	122.58° E	318:326	122.58° E	318:326	A Gram-stain-positive, endospore-forming, rod-shaped, motile and strictly aerobic strain, designated T3-209T, was isolated from sediment sampled at a water depth of 1206 m of the southern Okinawa Trough at station T1 (25.07° N, 122.58° E) near the Tangyin hydrothermal vent during an expedition on the R/V Kexue in May 2014.
29465340	1	74	attach	isolated	34:41	arg1	marine					48:53	marine	48:53	marine	48:53	nov., isolated from marine sediment near a hydrothermal vent.
29465340	1	74	attach	isolated	34:41	arg2	nov.					28:31	nov.	28:31	nov.	28:31	nov., isolated from marine sediment near a hydrothermal vent.
29465340	0	75	theme	alkalitolerans	9:22	arg1	sp					24:25	Bacillus alkalitolerans sp	0:25	Bacillus alkalitolerans sp.	0:26	Bacillus alkalitolerans sp.
29465340	1	76	theme	hydrothermal	71:82	arg1	vent					84:87	a hydrothermal vent	69:87	a hydrothermal vent	69:87	nov., isolated from marine sediment near a hydrothermal vent.
29465340	14	77	theme	29135T=MCCC	1648:1658	arg1	T3-209T					1622:1628	T3-209T	1622:1628	T3-209T (=KCTC 33631T=DSM 29135T=MCCC 1K00503T)	1622:1668	The type strain is T3-209T (=KCTC 33631T=DSM 29135T=MCCC 1K00503T).
29465340	14	77	theme	29135T=MCCC	1648:1658	arg1	1K00503T					1660:1667	=KCTC 33631T=DSM 29135T=MCCC 1K00503T	1631:1667	=KCTC 33631T=DSM 29135T=MCCC 1K00503T	1631:1667	The type strain is T3-209T (=KCTC 33631T=DSM 29135T=MCCC 1K00503T).
29465340	14	78	theme	=KCTC	1631:1635	arg1	T3-209T					1622:1628	T3-209T	1622:1628	T3-209T (=KCTC 33631T=DSM 29135T=MCCC 1K00503T)	1622:1668	The type strain is T3-209T (=KCTC 33631T=DSM 29135T=MCCC 1K00503T).
29465340	14	78	theme	=KCTC	1631:1635	arg1	1K00503T					1660:1667	=KCTC 33631T=DSM 29135T=MCCC 1K00503T	1631:1667	=KCTC 33631T=DSM 29135T=MCCC 1K00503T	1631:1667	The type strain is T3-209T (=KCTC 33631T=DSM 29135T=MCCC 1K00503T).
29465340	2	79	theme	aerobic	164:170	arg1	strain					172:177	A Gram-stain-positive, endospore-forming, rod-shaped, motile and strictly aerobic strain	90:177	A Gram-stain-positive, endospore-forming, rod-shaped, motile and strictly aerobic strain	90:177	A Gram-stain-positive, endospore-forming, rod-shaped, motile and strictly aerobic strain, designated T3-209T, was isolated from sediment sampled at a water depth of 1206 m of the southern Okinawa Trough at station T1 (25.07° N, 122.58° E) near the Tangyin hydrothermal vent during an expedition on the R/V Kexue in May 2014.
29465340	2	80	theme	hydrothermal	346:357	arg1	vent					359:362	the Tangyin hydrothermal vent	334:362	the Tangyin hydrothermal vent	334:362	A Gram-stain-positive, endospore-forming, rod-shaped, motile and strictly aerobic strain, designated T3-209T, was isolated from sediment sampled at a water depth of 1206 m of the southern Okinawa Trough at station T1 (25.07° N, 122.58° E) near the Tangyin hydrothermal vent during an expedition on the R/V Kexue in May 2014.
29465340	12	81	theme	phenotypic	1417:1426	arg1	characterization					1428:1443	phenotypic characterization	1417:1443	phenotypic characterization	1417:1443	Combining results of phylogenetic analysis, phenotypic characterization and chemotaxonomic studies, strain T3-209T represents a novel species of the genus Bacillus, for which the name Bacillus alkalitolerans sp.
29465340	6	82	with	related	767:773	arg1	%					886:886	97.0 %	881:886	97.0 %	881:886	The 16S rRNA gene sequence analysis indicated that strain T3-209T belonged to the genus Bacillus within the family Bacillaceae and was most closely related to Bacillus drentensis LMG 21831T and Bacillus bataviensis LMG 21833T, with the same sequence similarity (97.0 %), but shared relatively low levels of similarity (92.6-96.9 %) to the type strains of other Bacillus species.
29465340	6	82	with	related	767:773	arg1	similarity					869:878	the same sequence similarity	851:878	the same sequence similarity (97.0 %)	851:887	The 16S rRNA gene sequence analysis indicated that strain T3-209T belonged to the genus Bacillus within the family Bacillaceae and was most closely related to Bacillus drentensis LMG 21831T and Bacillus bataviensis LMG 21833T, with the same sequence similarity (97.0 %), but shared relatively low levels of similarity (92.6-96.9 %) to the type strains of other Bacillus species.
29465340	2	83	theme	station	296:302	arg1	T1					304:305	station T1	296:305	station T1 (25.07° N, 122.58° E) near the Tangyin hydrothermal vent during an expedition on the R/V Kexue in May 2014	296:412	A Gram-stain-positive, endospore-forming, rod-shaped, motile and strictly aerobic strain, designated T3-209T, was isolated from sediment sampled at a water depth of 1206 m of the southern Okinawa Trough at station T1 (25.07° N, 122.58° E) near the Tangyin hydrothermal vent during an expedition on the R/V Kexue in May 2014.
29465340	2	83	theme	station	296:302	arg1	N					315:315	25.07° N	308:315	25.07° N	308:315	A Gram-stain-positive, endospore-forming, rod-shaped, motile and strictly aerobic strain, designated T3-209T, was isolated from sediment sampled at a water depth of 1206 m of the southern Okinawa Trough at station T1 (25.07° N, 122.58° E) near the Tangyin hydrothermal vent during an expedition on the R/V Kexue in May 2014.
29465340	6	84	theme	strain	670:675	arg1	T3-209T					677:683	strain T3-209T	670:683	strain T3-209T	670:683	The 16S rRNA gene sequence analysis indicated that strain T3-209T belonged to the genus Bacillus within the family Bacillaceae and was most closely related to Bacillus drentensis LMG 21831T and Bacillus bataviensis LMG 21833T, with the same sequence similarity (97.0 %), but shared relatively low levels of similarity (92.6-96.9 %) to the type strains of other Bacillus species.
29465340	8	85	dep	anteiso-C15 	1111:1122	arg1	 0					1124:1125	 0	1124:1125	 0	1124:1125	The predominant cellular fatty acids were anteiso-C15 : 0, iso-C15 : 0 and alcohol-C16 : 1ω7c.
29465340	8	85	dep	anteiso-C15 	1111:1122	arg1	 1ω7c					1157:1161	 1ω7c	1157:1161	anteiso-C15 : 0, iso-C15 : 0 and alcohol-C16 : 1ω7c	1111:1161	The predominant cellular fatty acids were anteiso-C15 : 0, iso-C15 : 0 and alcohol-C16 : 1ω7c.
29465340	8	85	dep	anteiso-C15 	1111:1122	arg1	alcohol-C16 					1144:1155	alcohol-C16 	1144:1155	alcohol-C16 	1144:1155	The predominant cellular fatty acids were anteiso-C15 : 0, iso-C15 : 0 and alcohol-C16 : 1ω7c.
29465340	8	85	dep	anteiso-C15 	1111:1122	arg1	 0					1137:1138	 0	1137:1138	 0	1137:1138	The predominant cellular fatty acids were anteiso-C15 : 0, iso-C15 : 0 and alcohol-C16 : 1ω7c.
29465340	6	86	theme	similarity	926:935	arg1	levels					916:921	relatively low levels	901:921	relatively low levels of similarity (92.6-96.9 %) to the type strains of other Bacillus species	901:995	The 16S rRNA gene sequence analysis indicated that strain T3-209T belonged to the genus Bacillus within the family Bacillaceae and was most closely related to Bacillus drentensis LMG 21831T and Bacillus bataviensis LMG 21833T, with the same sequence similarity (97.0 %), but shared relatively low levels of similarity (92.6-96.9 %) to the type strains of other Bacillus species.
29465340	2	87	theme	122.58°	318:324	arg1	E					326:326	122.58° E	318:326	122.58° E	318:326	A Gram-stain-positive, endospore-forming, rod-shaped, motile and strictly aerobic strain, designated T3-209T, was isolated from sediment sampled at a water depth of 1206 m of the southern Okinawa Trough at station T1 (25.07° N, 122.58° E) near the Tangyin hydrothermal vent during an expedition on the R/V Kexue in May 2014.
31037822	4	0	theme	β-dicalcium	923:933	arg1	shell					963:967	self-curing β-dicalcium silicate (Ca2 SiO4 ) cement shell	911:967	self-curing β-dicalcium silicate (Ca2 SiO4 ) cement shell to form spherical granules (CaSiO3 @Ca2 SiO4 , MBG@Ca2 SiO4 )	911:1029	The introduction of β-calcium silicate (CaSiO3 ) or mesoporous bioactive glass (MBG) into self-curing β-dicalcium silicate (Ca2 SiO4 ) cement shell to form spherical granules (CaSiO3 @Ca2 SiO4 , MBG@Ca2 SiO4 ) was to retain the physicochemical property and/or microstructure of each component for optimizing bioactive ion release that could maximize osteostimulation in osteoporosis.
31037822	4	1	theme	physicochemical	1049:1063	arg1	property					1065:1072	the physicochemical property	1045:1072	the physicochemical property	1045:1072	The introduction of β-calcium silicate (CaSiO3 ) or mesoporous bioactive glass (MBG) into self-curing β-dicalcium silicate (Ca2 SiO4 ) cement shell to form spherical granules (CaSiO3 @Ca2 SiO4 , MBG@Ca2 SiO4 ) was to retain the physicochemical property and/or microstructure of each component for optimizing bioactive ion release that could maximize osteostimulation in osteoporosis.
31037822	2	2	theme	biological	372:381	arg1	performances					383:394	biological performances	372:394	biological performances of inorganic biomaterials	372:420	However, balancing the preparation conditions and biological performances of inorganic biomaterials remain a great challenge, especially when there is lack of biomaterial design on how to control component distribution and how pathological bone responds to the biomaterial stimulations and osteogenesis.
31037822	1	3	theme	past	269:272	arg1	decades					274:280	the past decades	265:280	the past decades	265:280	Application of bioactive materials as synthetic bone graft substitutes in regenerative medicine has seen great evolution over the past decades in treating challengeable bone defects.
31037822	7	4	theme	Extensive	1559:1567	arg1	evaluation					1590:1599	Extensive in vitro and in vivo evaluation	1559:1599	Extensive in vitro and in vivo evaluation	1559:1599	Extensive in vitro and in vivo evaluation showed that the CaSiO3 @Ca2 SiO4 and MBG@Ca2 SiO4 granules exhibited many superior properties such as controllable ion release, improved biodegradation and enhanced osteogenic capability in comparison with the pure Ca2 SiO4 @Ca2 SiO4 , thereby opening new mild-condition approach in fabricating osteogenesis-tailored silicate biomaterials for bone regenerative medicine, especially for efficient reconstruction of challenging pathological bone defects.
31037822	7	5	theme	mild-condition	1857:1870	arg1	approach					1872:1879	new mild-condition approach	1853:1879	new mild-condition approach in fabricating osteogenesis-tailored silicate biomaterials for bone regenerative medicine, especially for efficient reconstruction of challenging pathological bone defects	1853:2051	Extensive in vitro and in vivo evaluation showed that the CaSiO3 @Ca2 SiO4 and MBG@Ca2 SiO4 granules exhibited many superior properties such as controllable ion release, improved biodegradation and enhanced osteogenic capability in comparison with the pure Ca2 SiO4 @Ca2 SiO4 , thereby opening new mild-condition approach in fabricating osteogenesis-tailored silicate biomaterials for bone regenerative medicine, especially for efficient reconstruction of challenging pathological bone defects.
31037822	2	6	dep	biomaterial	583:593	arg1	stimulations					595:606	stimulations	595:606	stimulations	595:606	However, balancing the preparation conditions and biological performances of inorganic biomaterials remain a great challenge, especially when there is lack of biomaterial design on how to control component distribution and how pathological bone responds to the biomaterial stimulations and osteogenesis.
31037822	1	7	theme	bone	187:190	arg1	graft					192:196	synthetic bone graft substitutes	177:208	synthetic bone graft substitutes in regenerative medicine	177:233	Application of bioactive materials as synthetic bone graft substitutes in regenerative medicine has seen great evolution over the past decades in treating challengeable bone defects.
31037822	3	8	theme	defects	812:818	arg1	repair					784:789	repair	784:789	repair of osteoporotic bone defects	784:818	Here, our objective is to develop yolk-shell Ca-silicate microspheres and to investigate the potential biological performances to overcome the limitations in repair of osteoporotic bone defects.
31037822	7	9	theme	controllable	1703:1714	arg1	release					1720:1726	controllable ion release	1703:1726	controllable ion release	1703:1726	Extensive in vitro and in vivo evaluation showed that the CaSiO3 @Ca2 SiO4 and MBG@Ca2 SiO4 granules exhibited many superior properties such as controllable ion release, improved biodegradation and enhanced osteogenic capability in comparison with the pure Ca2 SiO4 @Ca2 SiO4 , thereby opening new mild-condition approach in fabricating osteogenesis-tailored silicate biomaterials for bone regenerative medicine, especially for efficient reconstruction of challenging pathological bone defects.
31037822	7	10	theme	Ca2	1826:1828	arg1	SiO4					1830:1833	the pure Ca2 SiO4 @Ca2 SiO4	1807:1833	the pure Ca2 SiO4 @Ca2 SiO4	1807:1833	Extensive in vitro and in vivo evaluation showed that the CaSiO3 @Ca2 SiO4 and MBG@Ca2 SiO4 granules exhibited many superior properties such as controllable ion release, improved biodegradation and enhanced osteogenic capability in comparison with the pure Ca2 SiO4 @Ca2 SiO4 , thereby opening new mild-condition approach in fabricating osteogenesis-tailored silicate biomaterials for bone regenerative medicine, especially for efficient reconstruction of challenging pathological bone defects.
31037822	5	11	from	phases	1325:1330	arg1	networks					1375:1382	interconnected macropore networks	1350:1382	interconnected macropore networks	1350:1382	We report a scalable shape-controlled mild fabrication protocol to yield the yolk-shell granules, endowing to different phases in yolk layer and interconnected macropore networks in the closely packed granule scaffolds.
31037822	5	11	from	phases	1325:1330	arg1	layer					1340:1344	yolk layer	1335:1344	yolk layer	1335:1344	We report a scalable shape-controlled mild fabrication protocol to yield the yolk-shell granules, endowing to different phases in yolk layer and interconnected macropore networks in the closely packed granule scaffolds.
31037822	3	12	theme	osteoporotic	794:805	arg1	defects					812:818	osteoporotic bone defects	794:818	osteoporotic bone defects	794:818	Here, our objective is to develop yolk-shell Ca-silicate microspheres and to investigate the potential biological performances to overcome the limitations in repair of osteoporotic bone defects.
31037822	0	13	theme	bone	126:129	arg1	defect					131:136	osteoporotic femoral bone defect	105:136	osteoporotic femoral bone defect	105:136	Composition control in biphasic silicate microspheres on stimulating new bone regeneration and repair of osteoporotic femoral bone defect.
31037822	1	14	theme	regenerative	213:224	arg1	medicine					226:233	regenerative medicine	213:233	regenerative medicine	213:233	Application of bioactive materials as synthetic bone graft substitutes in regenerative medicine has seen great evolution over the past decades in treating challengeable bone defects.
31037822	4	15	theme	spherical	977:985	arg1	granules					987:994	spherical granules	977:994	spherical granules (CaSiO3 @Ca2 SiO4 , MBG@Ca2 SiO4 )	977:1029	The introduction of β-calcium silicate (CaSiO3 ) or mesoporous bioactive glass (MBG) into self-curing β-dicalcium silicate (Ca2 SiO4 ) cement shell to form spherical granules (CaSiO3 @Ca2 SiO4 , MBG@Ca2 SiO4 ) was to retain the physicochemical property and/or microstructure of each component for optimizing bioactive ion release that could maximize osteostimulation in osteoporosis.
31037822	4	16	theme	ion	1139:1141	arg1	release					1143:1149	bioactive ion release	1129:1149	bioactive ion release that could maximize osteostimulation in osteoporosis	1129:1202	The introduction of β-calcium silicate (CaSiO3 ) or mesoporous bioactive glass (MBG) into self-curing β-dicalcium silicate (Ca2 SiO4 ) cement shell to form spherical granules (CaSiO3 @Ca2 SiO4 , MBG@Ca2 SiO4 ) was to retain the physicochemical property and/or microstructure of each component for optimizing bioactive ion release that could maximize osteostimulation in osteoporosis.
31037822	4	17	dep	granules	987:994	arg1	SiO4					1024:1027	MBG@Ca2 SiO4	1016:1027	MBG@Ca2 SiO4	1016:1027	The introduction of β-calcium silicate (CaSiO3 ) or mesoporous bioactive glass (MBG) into self-curing β-dicalcium silicate (Ca2 SiO4 ) cement shell to form spherical granules (CaSiO3 @Ca2 SiO4 , MBG@Ca2 SiO4 ) was to retain the physicochemical property and/or microstructure of each component for optimizing bioactive ion release that could maximize osteostimulation in osteoporosis.
31037822	4	17	dep	granules	987:994	arg1	SiO4					1009:1012	CaSiO3 @Ca2 SiO4	997:1012	CaSiO3 @Ca2 SiO4	997:1012	The introduction of β-calcium silicate (CaSiO3 ) or mesoporous bioactive glass (MBG) into self-curing β-dicalcium silicate (Ca2 SiO4 ) cement shell to form spherical granules (CaSiO3 @Ca2 SiO4 , MBG@Ca2 SiO4 ) was to retain the physicochemical property and/or microstructure of each component for optimizing bioactive ion release that could maximize osteostimulation in osteoporosis.
31037822	7	18	theme	superior	1675:1682	arg1	release					1720:1726	controllable ion release	1703:1726	controllable ion release	1703:1726	Extensive in vitro and in vivo evaluation showed that the CaSiO3 @Ca2 SiO4 and MBG@Ca2 SiO4 granules exhibited many superior properties such as controllable ion release, improved biodegradation and enhanced osteogenic capability in comparison with the pure Ca2 SiO4 @Ca2 SiO4 , thereby opening new mild-condition approach in fabricating osteogenesis-tailored silicate biomaterials for bone regenerative medicine, especially for efficient reconstruction of challenging pathological bone defects.
31037822	7	18	theme	superior	1675:1682	arg1	properties					1684:1693	many superior properties	1670:1693	many superior properties such as controllable ion release	1670:1726	Extensive in vitro and in vivo evaluation showed that the CaSiO3 @Ca2 SiO4 and MBG@Ca2 SiO4 granules exhibited many superior properties such as controllable ion release, improved biodegradation and enhanced osteogenic capability in comparison with the pure Ca2 SiO4 @Ca2 SiO4 , thereby opening new mild-condition approach in fabricating osteogenesis-tailored silicate biomaterials for bone regenerative medicine, especially for efficient reconstruction of challenging pathological bone defects.
31037822	4	19	theme	SiO4	949:952	arg1	shell					963:967	self-curing β-dicalcium silicate (Ca2 SiO4 ) cement shell	911:967	self-curing β-dicalcium silicate (Ca2 SiO4 ) cement shell to form spherical granules (CaSiO3 @Ca2 SiO4 , MBG@Ca2 SiO4 )	911:1029	The introduction of β-calcium silicate (CaSiO3 ) or mesoporous bioactive glass (MBG) into self-curing β-dicalcium silicate (Ca2 SiO4 ) cement shell to form spherical granules (CaSiO3 @Ca2 SiO4 , MBG@Ca2 SiO4 ) was to retain the physicochemical property and/or microstructure of each component for optimizing bioactive ion release that could maximize osteostimulation in osteoporosis.
31037822	7	20	theme	Ca2	1816:1818	arg1	SiO4					1830:1833	the pure Ca2 SiO4 @Ca2 SiO4	1807:1833	the pure Ca2 SiO4 @Ca2 SiO4	1807:1833	Extensive in vitro and in vivo evaluation showed that the CaSiO3 @Ca2 SiO4 and MBG@Ca2 SiO4 granules exhibited many superior properties such as controllable ion release, improved biodegradation and enhanced osteogenic capability in comparison with the pure Ca2 SiO4 @Ca2 SiO4 , thereby opening new mild-condition approach in fabricating osteogenesis-tailored silicate biomaterials for bone regenerative medicine, especially for efficient reconstruction of challenging pathological bone defects.
31037822	4	21	theme	cement	956:961	arg1	shell					963:967	self-curing β-dicalcium silicate (Ca2 SiO4 ) cement shell	911:967	self-curing β-dicalcium silicate (Ca2 SiO4 ) cement shell to form spherical granules (CaSiO3 @Ca2 SiO4 , MBG@Ca2 SiO4 )	911:1029	The introduction of β-calcium silicate (CaSiO3 ) or mesoporous bioactive glass (MBG) into self-curing β-dicalcium silicate (Ca2 SiO4 ) cement shell to form spherical granules (CaSiO3 @Ca2 SiO4 , MBG@Ca2 SiO4 ) was to retain the physicochemical property and/or microstructure of each component for optimizing bioactive ion release that could maximize osteostimulation in osteoporosis.
31037822	3	22	theme	yolk-shell	660:669	arg1	microspheres					683:694	yolk-shell Ca-silicate microspheres	660:694	yolk-shell Ca-silicate microspheres	660:694	Here, our objective is to develop yolk-shell Ca-silicate microspheres and to investigate the potential biological performances to overcome the limitations in repair of osteoporotic bone defects.
31037822	1	23	from	graft	192:196	arg1	medicine					226:233	regenerative medicine	213:233	regenerative medicine	213:233	Application of bioactive materials as synthetic bone graft substitutes in regenerative medicine has seen great evolution over the past decades in treating challengeable bone defects.
31037822	5	24	theme	interconnected	1350:1363	arg1	networks					1375:1382	interconnected macropore networks	1350:1382	interconnected macropore networks	1350:1382	We report a scalable shape-controlled mild fabrication protocol to yield the yolk-shell granules, endowing to different phases in yolk layer and interconnected macropore networks in the closely packed granule scaffolds.
31037822	7	25	theme	SiO4	1646:1649	arg1	granules					1651:1658	the CaSiO3 @Ca2 SiO4 and MBG@Ca2 SiO4 granules	1613:1658	the CaSiO3 @Ca2 SiO4 and MBG@Ca2 SiO4 granules	1613:1658	Extensive in vitro and in vivo evaluation showed that the CaSiO3 @Ca2 SiO4 and MBG@Ca2 SiO4 granules exhibited many superior properties such as controllable ion release, improved biodegradation and enhanced osteogenic capability in comparison with the pure Ca2 SiO4 @Ca2 SiO4 , thereby opening new mild-condition approach in fabricating osteogenesis-tailored silicate biomaterials for bone regenerative medicine, especially for efficient reconstruction of challenging pathological bone defects.
31037822	0	26	theme	new	69:71	arg1	regeneration					78:89	new bone regeneration	69:89	new bone regeneration	69:89	Composition control in biphasic silicate microspheres on stimulating new bone regeneration and repair of osteoporotic femoral bone defect.
31037822	1	27	theme	bioactive	154:162	arg1	materials					164:172	bioactive materials	154:172	bioactive materials	154:172	Application of bioactive materials as synthetic bone graft substitutes in regenerative medicine has seen great evolution over the past decades in treating challengeable bone defects.
31037822	7	28	theme	@	1641:1641	arg1	Ca2					1642:1644	MBG@Ca2	1638:1644	MBG@Ca2	1638:1644	Extensive in vitro and in vivo evaluation showed that the CaSiO3 @Ca2 SiO4 and MBG@Ca2 SiO4 granules exhibited many superior properties such as controllable ion release, improved biodegradation and enhanced osteogenic capability in comparison with the pure Ca2 SiO4 @Ca2 SiO4 , thereby opening new mild-condition approach in fabricating osteogenesis-tailored silicate biomaterials for bone regenerative medicine, especially for efficient reconstruction of challenging pathological bone defects.
31037822	4	29	theme	MBG	1016:1018	arg1	SiO4					1024:1027	MBG@Ca2 SiO4	1016:1027	MBG@Ca2 SiO4	1016:1027	The introduction of β-calcium silicate (CaSiO3 ) or mesoporous bioactive glass (MBG) into self-curing β-dicalcium silicate (Ca2 SiO4 ) cement shell to form spherical granules (CaSiO3 @Ca2 SiO4 , MBG@Ca2 SiO4 ) was to retain the physicochemical property and/or microstructure of each component for optimizing bioactive ion release that could maximize osteostimulation in osteoporosis.
31037822	4	29	theme	MBG	1016:1018	arg1	SiO4					1009:1012	CaSiO3 @Ca2 SiO4	997:1012	CaSiO3 @Ca2 SiO4	997:1012	The introduction of β-calcium silicate (CaSiO3 ) or mesoporous bioactive glass (MBG) into self-curing β-dicalcium silicate (Ca2 SiO4 ) cement shell to form spherical granules (CaSiO3 @Ca2 SiO4 , MBG@Ca2 SiO4 ) was to retain the physicochemical property and/or microstructure of each component for optimizing bioactive ion release that could maximize osteostimulation in osteoporosis.
31037822	1	30	theme	bone	308:311	arg1	defects					313:319	challengeable bone defects	294:319	challengeable bone defects	294:319	Application of bioactive materials as synthetic bone graft substitutes in regenerative medicine has seen great evolution over the past decades in treating challengeable bone defects.
31037822	5	31	theme	endowing	1303:1310	arg1	granules					1293:1300	the yolk-shell granules	1278:1300	the yolk-shell granules	1278:1300	We report a scalable shape-controlled mild fabrication protocol to yield the yolk-shell granules, endowing to different phases in yolk layer and interconnected macropore networks in the closely packed granule scaffolds.
31037822	6	32	theme	unique	1430:1435	arg1	preparation					1453:1463	This unique heterostructure preparation	1425:1463	This unique heterostructure preparation	1425:1463	This unique heterostructure preparation is governed by coaxially aligned bilayer nozzle, inorganic powders and biocompatible binders.
31037822	5	33	theme	different	1315:1323	arg1	phases					1325:1330	different phases	1315:1330	different phases in yolk layer and interconnected macropore networks in the closely packed granule scaffolds	1315:1422	We report a scalable shape-controlled mild fabrication protocol to yield the yolk-shell granules, endowing to different phases in yolk layer and interconnected macropore networks in the closely packed granule scaffolds.
31037822	7	34	theme	Ca2	1625:1627	arg1	SiO4					1629:1632	the CaSiO3 @Ca2 SiO4	1613:1632	the CaSiO3 @Ca2 SiO4	1613:1632	Extensive in vitro and in vivo evaluation showed that the CaSiO3 @Ca2 SiO4 and MBG@Ca2 SiO4 granules exhibited many superior properties such as controllable ion release, improved biodegradation and enhanced osteogenic capability in comparison with the pure Ca2 SiO4 @Ca2 SiO4 , thereby opening new mild-condition approach in fabricating osteogenesis-tailored silicate biomaterials for bone regenerative medicine, especially for efficient reconstruction of challenging pathological bone defects.
31037822	4	35	theme	@	1004:1004	arg1	SiO4					1024:1027	MBG@Ca2 SiO4	1016:1027	MBG@Ca2 SiO4	1016:1027	The introduction of β-calcium silicate (CaSiO3 ) or mesoporous bioactive glass (MBG) into self-curing β-dicalcium silicate (Ca2 SiO4 ) cement shell to form spherical granules (CaSiO3 @Ca2 SiO4 , MBG@Ca2 SiO4 ) was to retain the physicochemical property and/or microstructure of each component for optimizing bioactive ion release that could maximize osteostimulation in osteoporosis.
31037822	4	35	theme	@	1004:1004	arg1	SiO4					1009:1012	CaSiO3 @Ca2 SiO4	997:1012	CaSiO3 @Ca2 SiO4	997:1012	The introduction of β-calcium silicate (CaSiO3 ) or mesoporous bioactive glass (MBG) into self-curing β-dicalcium silicate (Ca2 SiO4 ) cement shell to form spherical granules (CaSiO3 @Ca2 SiO4 , MBG@Ca2 SiO4 ) was to retain the physicochemical property and/or microstructure of each component for optimizing bioactive ion release that could maximize osteostimulation in osteoporosis.
31037822	4	36	theme	β-calcium	841:849	arg1	CaSiO3					861:866	CaSiO3	861:866	CaSiO3	861:866	The introduction of β-calcium silicate (CaSiO3 ) or mesoporous bioactive glass (MBG) into self-curing β-dicalcium silicate (Ca2 SiO4 ) cement shell to form spherical granules (CaSiO3 @Ca2 SiO4 , MBG@Ca2 SiO4 ) was to retain the physicochemical property and/or microstructure of each component for optimizing bioactive ion release that could maximize osteostimulation in osteoporosis.
31037822	4	36	theme	β-calcium	841:849	arg1	silicate					851:858	β-calcium silicate	841:858	β-calcium silicate (CaSiO3 )	841:868	The introduction of β-calcium silicate (CaSiO3 ) or mesoporous bioactive glass (MBG) into self-curing β-dicalcium silicate (Ca2 SiO4 ) cement shell to form spherical granules (CaSiO3 @Ca2 SiO4 , MBG@Ca2 SiO4 ) was to retain the physicochemical property and/or microstructure of each component for optimizing bioactive ion release that could maximize osteostimulation in osteoporosis.
31037822	2	37	theme	great	431:435	arg1	challenge					437:445	a great challenge	429:445	a great challenge	429:445	However, balancing the preparation conditions and biological performances of inorganic biomaterials remain a great challenge, especially when there is lack of biomaterial design on how to control component distribution and how pathological bone responds to the biomaterial stimulations and osteogenesis.
31037822	0	38	theme	osteoporotic	105:116	arg1	defect					131:136	osteoporotic femoral bone defect	105:136	osteoporotic femoral bone defect	105:136	Composition control in biphasic silicate microspheres on stimulating new bone regeneration and repair of osteoporotic femoral bone defect.
31037822	7	39	dep	in	1582:1583	arg1	vivo					1585:1588	vivo	1585:1588	vivo	1585:1588	Extensive in vitro and in vivo evaluation showed that the CaSiO3 @Ca2 SiO4 and MBG@Ca2 SiO4 granules exhibited many superior properties such as controllable ion release, improved biodegradation and enhanced osteogenic capability in comparison with the pure Ca2 SiO4 @Ca2 SiO4 , thereby opening new mild-condition approach in fabricating osteogenesis-tailored silicate biomaterials for bone regenerative medicine, especially for efficient reconstruction of challenging pathological bone defects.
31037822	5	40	theme	packed	1399:1404	arg1	scaffolds					1414:1422	the closely packed granule scaffolds	1387:1422	the closely packed granule scaffolds	1387:1422	We report a scalable shape-controlled mild fabrication protocol to yield the yolk-shell granules, endowing to different phases in yolk layer and interconnected macropore networks in the closely packed granule scaffolds.
31037822	7	41	theme	pathological	2027:2038	arg1	defects					2045:2051	pathological bone defects	2027:2051	pathological bone defects	2027:2051	Extensive in vitro and in vivo evaluation showed that the CaSiO3 @Ca2 SiO4 and MBG@Ca2 SiO4 granules exhibited many superior properties such as controllable ion release, improved biodegradation and enhanced osteogenic capability in comparison with the pure Ca2 SiO4 @Ca2 SiO4 , thereby opening new mild-condition approach in fabricating osteogenesis-tailored silicate biomaterials for bone regenerative medicine, especially for efficient reconstruction of challenging pathological bone defects.
31037822	1	42	dep	graft	192:196	arg1	substitutes					198:208	substitutes	198:208	substitutes	198:208	Application of bioactive materials as synthetic bone graft substitutes in regenerative medicine has seen great evolution over the past decades in treating challengeable bone defects.
31037822	6	43	theme	bilayer	1498:1504	arg1	nozzle					1506:1511	coaxially aligned bilayer nozzle	1480:1511	coaxially aligned bilayer nozzle	1480:1511	This unique heterostructure preparation is governed by coaxially aligned bilayer nozzle, inorganic powders and biocompatible binders.
31037822	5	44	theme	scalable	1217:1224	arg1	protocol					1260:1267	a scalable shape-controlled mild fabrication protocol	1215:1267	a scalable shape-controlled mild fabrication protocol to yield the yolk-shell granules, endowing to different phases in yolk layer and interconnected macropore networks in the closely packed granule scaffolds	1215:1422	We report a scalable shape-controlled mild fabrication protocol to yield the yolk-shell granules, endowing to different phases in yolk layer and interconnected macropore networks in the closely packed granule scaffolds.
31037822	7	45	theme	ion	1716:1718	arg1	release					1720:1726	controllable ion release	1703:1726	controllable ion release	1703:1726	Extensive in vitro and in vivo evaluation showed that the CaSiO3 @Ca2 SiO4 and MBG@Ca2 SiO4 granules exhibited many superior properties such as controllable ion release, improved biodegradation and enhanced osteogenic capability in comparison with the pure Ca2 SiO4 @Ca2 SiO4 , thereby opening new mild-condition approach in fabricating osteogenesis-tailored silicate biomaterials for bone regenerative medicine, especially for efficient reconstruction of challenging pathological bone defects.
31037822	4	46	theme	Ca2	1020:1022	arg1	SiO4					1024:1027	MBG@Ca2 SiO4	1016:1027	MBG@Ca2 SiO4	1016:1027	The introduction of β-calcium silicate (CaSiO3 ) or mesoporous bioactive glass (MBG) into self-curing β-dicalcium silicate (Ca2 SiO4 ) cement shell to form spherical granules (CaSiO3 @Ca2 SiO4 , MBG@Ca2 SiO4 ) was to retain the physicochemical property and/or microstructure of each component for optimizing bioactive ion release that could maximize osteostimulation in osteoporosis.
31037822	4	46	theme	Ca2	1020:1022	arg1	SiO4					1009:1012	CaSiO3 @Ca2 SiO4	997:1012	CaSiO3 @Ca2 SiO4	997:1012	The introduction of β-calcium silicate (CaSiO3 ) or mesoporous bioactive glass (MBG) into self-curing β-dicalcium silicate (Ca2 SiO4 ) cement shell to form spherical granules (CaSiO3 @Ca2 SiO4 , MBG@Ca2 SiO4 ) was to retain the physicochemical property and/or microstructure of each component for optimizing bioactive ion release that could maximize osteostimulation in osteoporosis.
31037822	4	47	theme	mesoporous	873:882	arg1	MBG					901:903	MBG	901:903	MBG	901:903	The introduction of β-calcium silicate (CaSiO3 ) or mesoporous bioactive glass (MBG) into self-curing β-dicalcium silicate (Ca2 SiO4 ) cement shell to form spherical granules (CaSiO3 @Ca2 SiO4 , MBG@Ca2 SiO4 ) was to retain the physicochemical property and/or microstructure of each component for optimizing bioactive ion release that could maximize osteostimulation in osteoporosis.
31037822	4	47	theme	mesoporous	873:882	arg1	glass					894:898	mesoporous bioactive glass	873:898	mesoporous bioactive glass (MBG)	873:904	The introduction of β-calcium silicate (CaSiO3 ) or mesoporous bioactive glass (MBG) into self-curing β-dicalcium silicate (Ca2 SiO4 ) cement shell to form spherical granules (CaSiO3 @Ca2 SiO4 , MBG@Ca2 SiO4 ) was to retain the physicochemical property and/or microstructure of each component for optimizing bioactive ion release that could maximize osteostimulation in osteoporosis.
31037822	1	48	theme	synthetic	177:185	arg1	graft					192:196	synthetic bone graft substitutes	177:208	synthetic bone graft substitutes in regenerative medicine	177:233	Application of bioactive materials as synthetic bone graft substitutes in regenerative medicine has seen great evolution over the past decades in treating challengeable bone defects.
31037822	0	49	theme	silicate	32:39	arg1	microspheres					41:52	biphasic silicate microspheres	23:52	biphasic silicate microspheres	23:52	Composition control in biphasic silicate microspheres on stimulating new bone regeneration and repair of osteoporotic femoral bone defect.
31037822	2	50	theme	design	493:498	arg1	lack					473:476	lack	473:476	lack of biomaterial design	473:498	However, balancing the preparation conditions and biological performances of inorganic biomaterials remain a great challenge, especially when there is lack of biomaterial design on how to control component distribution and how pathological bone responds to the biomaterial stimulations and osteogenesis.
31037822	2	51	theme	component	518:526	arg1	distribution					528:539	component distribution	518:539	component distribution	518:539	However, balancing the preparation conditions and biological performances of inorganic biomaterials remain a great challenge, especially when there is lack of biomaterial design on how to control component distribution and how pathological bone responds to the biomaterial stimulations and osteogenesis.
31037822	4	52	theme	glass	894:898	arg1	introduction					825:836	The introduction	821:836	The introduction of β-calcium silicate (CaSiO3 ) or mesoporous bioactive glass (MBG) into self-curing β-dicalcium silicate (Ca2 SiO4 ) cement shell to form spherical granules (CaSiO3 @Ca2 SiO4 , MBG@Ca2 SiO4 )	821:1029	The introduction of β-calcium silicate (CaSiO3 ) or mesoporous bioactive glass (MBG) into self-curing β-dicalcium silicate (Ca2 SiO4 ) cement shell to form spherical granules (CaSiO3 @Ca2 SiO4 , MBG@Ca2 SiO4 ) was to retain the physicochemical property and/or microstructure of each component for optimizing bioactive ion release that could maximize osteostimulation in osteoporosis.
31037822	7	53	theme	efficient	1987:1995	arg1	reconstruction					1997:2010	efficient reconstruction	1987:2010	efficient reconstruction of challenging pathological bone defects	1987:2051	Extensive in vitro and in vivo evaluation showed that the CaSiO3 @Ca2 SiO4 and MBG@Ca2 SiO4 granules exhibited many superior properties such as controllable ion release, improved biodegradation and enhanced osteogenic capability in comparison with the pure Ca2 SiO4 @Ca2 SiO4 , thereby opening new mild-condition approach in fabricating osteogenesis-tailored silicate biomaterials for bone regenerative medicine, especially for efficient reconstruction of challenging pathological bone defects.
31037822	7	54	theme	silicate	1918:1925	arg1	biomaterials					1927:1938	fabricating osteogenesis-tailored silicate biomaterials	1884:1938	fabricating osteogenesis-tailored silicate biomaterials	1884:1938	Extensive in vitro and in vivo evaluation showed that the CaSiO3 @Ca2 SiO4 and MBG@Ca2 SiO4 granules exhibited many superior properties such as controllable ion release, improved biodegradation and enhanced osteogenic capability in comparison with the pure Ca2 SiO4 @Ca2 SiO4 , thereby opening new mild-condition approach in fabricating osteogenesis-tailored silicate biomaterials for bone regenerative medicine, especially for efficient reconstruction of challenging pathological bone defects.
31037822	3	55	theme	biological	729:738	arg1	performances					740:751	the potential biological performances	715:751	the potential biological performances	715:751	Here, our objective is to develop yolk-shell Ca-silicate microspheres and to investigate the potential biological performances to overcome the limitations in repair of osteoporotic bone defects.
31037822	2	56	dep	remain	422:427	arg1	balancing					331:339	balancing	331:339	balancing the preparation conditions	331:366	However, balancing the preparation conditions and biological performances of inorganic biomaterials remain a great challenge, especially when there is lack of biomaterial design on how to control component distribution and how pathological bone responds to the biomaterial stimulations and osteogenesis.
31037822	2	57	theme	preparation	345:355	arg1	conditions					357:366	the preparation conditions	341:366	the preparation conditions	341:366	However, balancing the preparation conditions and biological performances of inorganic biomaterials remain a great challenge, especially when there is lack of biomaterial design on how to control component distribution and how pathological bone responds to the biomaterial stimulations and osteogenesis.
31037822	7	58	theme	fabricating	1884:1894	arg1	biomaterials					1927:1938	fabricating osteogenesis-tailored silicate biomaterials	1884:1938	fabricating osteogenesis-tailored silicate biomaterials	1884:1938	Extensive in vitro and in vivo evaluation showed that the CaSiO3 @Ca2 SiO4 and MBG@Ca2 SiO4 granules exhibited many superior properties such as controllable ion release, improved biodegradation and enhanced osteogenic capability in comparison with the pure Ca2 SiO4 @Ca2 SiO4 , thereby opening new mild-condition approach in fabricating osteogenesis-tailored silicate biomaterials for bone regenerative medicine, especially for efficient reconstruction of challenging pathological bone defects.
31037822	2	59	theme	inorganic	399:407	arg1	biomaterials					409:420	inorganic biomaterials	399:420	inorganic biomaterials	399:420	However, balancing the preparation conditions and biological performances of inorganic biomaterials remain a great challenge, especially when there is lack of biomaterial design on how to control component distribution and how pathological bone responds to the biomaterial stimulations and osteogenesis.
31037822	5	60	theme	yolk-shell	1282:1291	arg1	granules					1293:1300	the yolk-shell granules	1278:1300	the yolk-shell granules	1278:1300	We report a scalable shape-controlled mild fabrication protocol to yield the yolk-shell granules, endowing to different phases in yolk layer and interconnected macropore networks in the closely packed granule scaffolds.
31037822	7	61	theme	in	1569:1570	arg1	evaluation					1590:1599	Extensive in vitro and in vivo evaluation	1559:1599	Extensive in vitro and in vivo evaluation	1559:1599	Extensive in vitro and in vivo evaluation showed that the CaSiO3 @Ca2 SiO4 and MBG@Ca2 SiO4 granules exhibited many superior properties such as controllable ion release, improved biodegradation and enhanced osteogenic capability in comparison with the pure Ca2 SiO4 @Ca2 SiO4 , thereby opening new mild-condition approach in fabricating osteogenesis-tailored silicate biomaterials for bone regenerative medicine, especially for efficient reconstruction of challenging pathological bone defects.
31037822	7	62	dep	in	1569:1570	arg1	vitro					1572:1576	vitro	1572:1576	vitro	1572:1576	Extensive in vitro and in vivo evaluation showed that the CaSiO3 @Ca2 SiO4 and MBG@Ca2 SiO4 granules exhibited many superior properties such as controllable ion release, improved biodegradation and enhanced osteogenic capability in comparison with the pure Ca2 SiO4 @Ca2 SiO4 , thereby opening new mild-condition approach in fabricating osteogenesis-tailored silicate biomaterials for bone regenerative medicine, especially for efficient reconstruction of challenging pathological bone defects.
31037822	6	63	theme	biocompatible	1536:1548	arg1	binders					1550:1556	biocompatible binders	1536:1556	biocompatible binders	1536:1556	This unique heterostructure preparation is governed by coaxially aligned bilayer nozzle, inorganic powders and biocompatible binders.
31037822	5	64	theme	mild	1243:1246	arg1	protocol					1260:1267	a scalable shape-controlled mild fabrication protocol	1215:1267	a scalable shape-controlled mild fabrication protocol to yield the yolk-shell granules, endowing to different phases in yolk layer and interconnected macropore networks in the closely packed granule scaffolds	1215:1422	We report a scalable shape-controlled mild fabrication protocol to yield the yolk-shell granules, endowing to different phases in yolk layer and interconnected macropore networks in the closely packed granule scaffolds.
31037822	4	65	theme	self-curing	911:921	arg1	shell					963:967	self-curing β-dicalcium silicate (Ca2 SiO4 ) cement shell	911:967	self-curing β-dicalcium silicate (Ca2 SiO4 ) cement shell to form spherical granules (CaSiO3 @Ca2 SiO4 , MBG@Ca2 SiO4 )	911:1029	The introduction of β-calcium silicate (CaSiO3 ) or mesoporous bioactive glass (MBG) into self-curing β-dicalcium silicate (Ca2 SiO4 ) cement shell to form spherical granules (CaSiO3 @Ca2 SiO4 , MBG@Ca2 SiO4 ) was to retain the physicochemical property and/or microstructure of each component for optimizing bioactive ion release that could maximize osteostimulation in osteoporosis.
31037822	7	66	theme	new	1853:1855	arg1	approach					1872:1879	new mild-condition approach	1853:1879	new mild-condition approach in fabricating osteogenesis-tailored silicate biomaterials for bone regenerative medicine, especially for efficient reconstruction of challenging pathological bone defects	1853:2051	Extensive in vitro and in vivo evaluation showed that the CaSiO3 @Ca2 SiO4 and MBG@Ca2 SiO4 granules exhibited many superior properties such as controllable ion release, improved biodegradation and enhanced osteogenic capability in comparison with the pure Ca2 SiO4 @Ca2 SiO4 , thereby opening new mild-condition approach in fabricating osteogenesis-tailored silicate biomaterials for bone regenerative medicine, especially for efficient reconstruction of challenging pathological bone defects.
31037822	7	67	dep	SiO4	1830:1833	arg1	opening					1845:1851	opening	1845:1851	opening	1845:1851	Extensive in vitro and in vivo evaluation showed that the CaSiO3 @Ca2 SiO4 and MBG@Ca2 SiO4 granules exhibited many superior properties such as controllable ion release, improved biodegradation and enhanced osteogenic capability in comparison with the pure Ca2 SiO4 @Ca2 SiO4 , thereby opening new mild-condition approach in fabricating osteogenesis-tailored silicate biomaterials for bone regenerative medicine, especially for efficient reconstruction of challenging pathological bone defects.
31037822	4	68	theme	silicate	935:942	arg1	shell					963:967	self-curing β-dicalcium silicate (Ca2 SiO4 ) cement shell	911:967	self-curing β-dicalcium silicate (Ca2 SiO4 ) cement shell to form spherical granules (CaSiO3 @Ca2 SiO4 , MBG@Ca2 SiO4 )	911:1029	The introduction of β-calcium silicate (CaSiO3 ) or mesoporous bioactive glass (MBG) into self-curing β-dicalcium silicate (Ca2 SiO4 ) cement shell to form spherical granules (CaSiO3 @Ca2 SiO4 , MBG@Ca2 SiO4 ) was to retain the physicochemical property and/or microstructure of each component for optimizing bioactive ion release that could maximize osteostimulation in osteoporosis.
31037822	4	69	from	osteostimulation	1171:1186	arg1	osteoporosis					1191:1202	osteoporosis	1191:1202	osteoporosis	1191:1202	The introduction of β-calcium silicate (CaSiO3 ) or mesoporous bioactive glass (MBG) into self-curing β-dicalcium silicate (Ca2 SiO4 ) cement shell to form spherical granules (CaSiO3 @Ca2 SiO4 , MBG@Ca2 SiO4 ) was to retain the physicochemical property and/or microstructure of each component for optimizing bioactive ion release that could maximize osteostimulation in osteoporosis.
31037822	3	70	from	limitations	769:779	arg1	repair					784:789	repair	784:789	repair of osteoporotic bone defects	784:818	Here, our objective is to develop yolk-shell Ca-silicate microspheres and to investigate the potential biological performances to overcome the limitations in repair of osteoporotic bone defects.
31037822	7	71	theme	@	1825:1825	arg1	SiO4					1830:1833	the pure Ca2 SiO4 @Ca2 SiO4	1807:1833	the pure Ca2 SiO4 @Ca2 SiO4	1807:1833	Extensive in vitro and in vivo evaluation showed that the CaSiO3 @Ca2 SiO4 and MBG@Ca2 SiO4 granules exhibited many superior properties such as controllable ion release, improved biodegradation and enhanced osteogenic capability in comparison with the pure Ca2 SiO4 @Ca2 SiO4 , thereby opening new mild-condition approach in fabricating osteogenesis-tailored silicate biomaterials for bone regenerative medicine, especially for efficient reconstruction of challenging pathological bone defects.
31037822	5	72	from	layer	1340:1344	arg1	scaffolds					1414:1422	the closely packed granule scaffolds	1387:1422	the closely packed granule scaffolds	1387:1422	We report a scalable shape-controlled mild fabrication protocol to yield the yolk-shell granules, endowing to different phases in yolk layer and interconnected macropore networks in the closely packed granule scaffolds.
31037822	4	73	theme	bioactive	1129:1137	arg1	release					1143:1149	bioactive ion release	1129:1149	bioactive ion release that could maximize osteostimulation in osteoporosis	1129:1202	The introduction of β-calcium silicate (CaSiO3 ) or mesoporous bioactive glass (MBG) into self-curing β-dicalcium silicate (Ca2 SiO4 ) cement shell to form spherical granules (CaSiO3 @Ca2 SiO4 , MBG@Ca2 SiO4 ) was to retain the physicochemical property and/or microstructure of each component for optimizing bioactive ion release that could maximize osteostimulation in osteoporosis.
31037822	0	74	theme	defect	131:136	arg1	regeneration					78:89	new bone regeneration	69:89	new bone regeneration	69:89	Composition control in biphasic silicate microspheres on stimulating new bone regeneration and repair of osteoporotic femoral bone defect.
31037822	0	74	theme	defect	131:136	arg1	repair					95:100	repair	95:100	repair	95:100	Composition control in biphasic silicate microspheres on stimulating new bone regeneration and repair of osteoporotic femoral bone defect.
31037822	3	75	theme	bone	807:810	arg1	defects					812:818	osteoporotic bone defects	794:818	osteoporotic bone defects	794:818	Here, our objective is to develop yolk-shell Ca-silicate microspheres and to investigate the potential biological performances to overcome the limitations in repair of osteoporotic bone defects.
31037822	7	76	theme	many	1670:1673	arg1	release					1720:1726	controllable ion release	1703:1726	controllable ion release	1703:1726	Extensive in vitro and in vivo evaluation showed that the CaSiO3 @Ca2 SiO4 and MBG@Ca2 SiO4 granules exhibited many superior properties such as controllable ion release, improved biodegradation and enhanced osteogenic capability in comparison with the pure Ca2 SiO4 @Ca2 SiO4 , thereby opening new mild-condition approach in fabricating osteogenesis-tailored silicate biomaterials for bone regenerative medicine, especially for efficient reconstruction of challenging pathological bone defects.
31037822	7	76	theme	many	1670:1673	arg1	properties					1684:1693	many superior properties	1670:1693	many superior properties such as controllable ion release	1670:1726	Extensive in vitro and in vivo evaluation showed that the CaSiO3 @Ca2 SiO4 and MBG@Ca2 SiO4 granules exhibited many superior properties such as controllable ion release, improved biodegradation and enhanced osteogenic capability in comparison with the pure Ca2 SiO4 @Ca2 SiO4 , thereby opening new mild-condition approach in fabricating osteogenesis-tailored silicate biomaterials for bone regenerative medicine, especially for efficient reconstruction of challenging pathological bone defects.
31037822	5	77	from	networks	1375:1382	arg1	scaffolds					1414:1422	the closely packed granule scaffolds	1387:1422	the closely packed granule scaffolds	1387:1422	We report a scalable shape-controlled mild fabrication protocol to yield the yolk-shell granules, endowing to different phases in yolk layer and interconnected macropore networks in the closely packed granule scaffolds.
31037822	4	78	theme	Ca2	945:947	arg1	shell					963:967	self-curing β-dicalcium silicate (Ca2 SiO4 ) cement shell	911:967	self-curing β-dicalcium silicate (Ca2 SiO4 ) cement shell to form spherical granules (CaSiO3 @Ca2 SiO4 , MBG@Ca2 SiO4 )	911:1029	The introduction of β-calcium silicate (CaSiO3 ) or mesoporous bioactive glass (MBG) into self-curing β-dicalcium silicate (Ca2 SiO4 ) cement shell to form spherical granules (CaSiO3 @Ca2 SiO4 , MBG@Ca2 SiO4 ) was to retain the physicochemical property and/or microstructure of each component for optimizing bioactive ion release that could maximize osteostimulation in osteoporosis.
31037822	7	79	theme	SiO4	1820:1823	arg1	SiO4					1830:1833	the pure Ca2 SiO4 @Ca2 SiO4	1807:1833	the pure Ca2 SiO4 @Ca2 SiO4	1807:1833	Extensive in vitro and in vivo evaluation showed that the CaSiO3 @Ca2 SiO4 and MBG@Ca2 SiO4 granules exhibited many superior properties such as controllable ion release, improved biodegradation and enhanced osteogenic capability in comparison with the pure Ca2 SiO4 @Ca2 SiO4 , thereby opening new mild-condition approach in fabricating osteogenesis-tailored silicate biomaterials for bone regenerative medicine, especially for efficient reconstruction of challenging pathological bone defects.
31037822	7	80	theme	Ca2	1642:1644	arg1	granules					1651:1658	the CaSiO3 @Ca2 SiO4 and MBG@Ca2 SiO4 granules	1613:1658	the CaSiO3 @Ca2 SiO4 and MBG@Ca2 SiO4 granules	1613:1658	Extensive in vitro and in vivo evaluation showed that the CaSiO3 @Ca2 SiO4 and MBG@Ca2 SiO4 granules exhibited many superior properties such as controllable ion release, improved biodegradation and enhanced osteogenic capability in comparison with the pure Ca2 SiO4 @Ca2 SiO4 , thereby opening new mild-condition approach in fabricating osteogenesis-tailored silicate biomaterials for bone regenerative medicine, especially for efficient reconstruction of challenging pathological bone defects.
31037822	5	81	theme	yolk	1335:1338	arg1	layer					1340:1344	yolk layer	1335:1344	yolk layer	1335:1344	We report a scalable shape-controlled mild fabrication protocol to yield the yolk-shell granules, endowing to different phases in yolk layer and interconnected macropore networks in the closely packed granule scaffolds.
31037822	0	82	theme	bone	73:76	arg1	regeneration					78:89	new bone regeneration	69:89	new bone regeneration	69:89	Composition control in biphasic silicate microspheres on stimulating new bone regeneration and repair of osteoporotic femoral bone defect.
31037822	7	83	theme	pure	1811:1814	arg1	SiO4					1830:1833	the pure Ca2 SiO4 @Ca2 SiO4	1807:1833	the pure Ca2 SiO4 @Ca2 SiO4	1807:1833	Extensive in vitro and in vivo evaluation showed that the CaSiO3 @Ca2 SiO4 and MBG@Ca2 SiO4 granules exhibited many superior properties such as controllable ion release, improved biodegradation and enhanced osteogenic capability in comparison with the pure Ca2 SiO4 @Ca2 SiO4 , thereby opening new mild-condition approach in fabricating osteogenesis-tailored silicate biomaterials for bone regenerative medicine, especially for efficient reconstruction of challenging pathological bone defects.
31037822	7	84	theme	MBG	1638:1640	arg1	Ca2					1642:1644	MBG@Ca2	1638:1644	MBG@Ca2	1638:1644	Extensive in vitro and in vivo evaluation showed that the CaSiO3 @Ca2 SiO4 and MBG@Ca2 SiO4 granules exhibited many superior properties such as controllable ion release, improved biodegradation and enhanced osteogenic capability in comparison with the pure Ca2 SiO4 @Ca2 SiO4 , thereby opening new mild-condition approach in fabricating osteogenesis-tailored silicate biomaterials for bone regenerative medicine, especially for efficient reconstruction of challenging pathological bone defects.
31037822	4	85	theme	Ca2	1005:1007	arg1	SiO4					1024:1027	MBG@Ca2 SiO4	1016:1027	MBG@Ca2 SiO4	1016:1027	The introduction of β-calcium silicate (CaSiO3 ) or mesoporous bioactive glass (MBG) into self-curing β-dicalcium silicate (Ca2 SiO4 ) cement shell to form spherical granules (CaSiO3 @Ca2 SiO4 , MBG@Ca2 SiO4 ) was to retain the physicochemical property and/or microstructure of each component for optimizing bioactive ion release that could maximize osteostimulation in osteoporosis.
31037822	4	85	theme	Ca2	1005:1007	arg1	SiO4					1009:1012	CaSiO3 @Ca2 SiO4	997:1012	CaSiO3 @Ca2 SiO4	997:1012	The introduction of β-calcium silicate (CaSiO3 ) or mesoporous bioactive glass (MBG) into self-curing β-dicalcium silicate (Ca2 SiO4 ) cement shell to form spherical granules (CaSiO3 @Ca2 SiO4 , MBG@Ca2 SiO4 ) was to retain the physicochemical property and/or microstructure of each component for optimizing bioactive ion release that could maximize osteostimulation in osteoporosis.
31037822	6	86	theme	heterostructure	1437:1451	arg1	preparation					1453:1463	This unique heterostructure preparation	1425:1463	This unique heterostructure preparation	1425:1463	This unique heterostructure preparation is governed by coaxially aligned bilayer nozzle, inorganic powders and biocompatible binders.
31037822	1	87	theme	materials	164:172	arg1	Application					139:149	Application	139:149	Application of bioactive materials as synthetic bone graft substitutes in regenerative medicine	139:233	Application of bioactive materials as synthetic bone graft substitutes in regenerative medicine has seen great evolution over the past decades in treating challengeable bone defects.
31037822	7	88	theme	SiO4	1629:1632	arg1	granules					1651:1658	the CaSiO3 @Ca2 SiO4 and MBG@Ca2 SiO4 granules	1613:1658	the CaSiO3 @Ca2 SiO4 and MBG@Ca2 SiO4 granules	1613:1658	Extensive in vitro and in vivo evaluation showed that the CaSiO3 @Ca2 SiO4 and MBG@Ca2 SiO4 granules exhibited many superior properties such as controllable ion release, improved biodegradation and enhanced osteogenic capability in comparison with the pure Ca2 SiO4 @Ca2 SiO4 , thereby opening new mild-condition approach in fabricating osteogenesis-tailored silicate biomaterials for bone regenerative medicine, especially for efficient reconstruction of challenging pathological bone defects.
31037822	7	89	with	comparison	1791:1800	arg1	SiO4					1830:1833	the pure Ca2 SiO4 @Ca2 SiO4	1807:1833	the pure Ca2 SiO4 @Ca2 SiO4	1807:1833	Extensive in vitro and in vivo evaluation showed that the CaSiO3 @Ca2 SiO4 and MBG@Ca2 SiO4 granules exhibited many superior properties such as controllable ion release, improved biodegradation and enhanced osteogenic capability in comparison with the pure Ca2 SiO4 @Ca2 SiO4 , thereby opening new mild-condition approach in fabricating osteogenesis-tailored silicate biomaterials for bone regenerative medicine, especially for efficient reconstruction of challenging pathological bone defects.
31037822	7	90	theme	@	1624:1624	arg1	SiO4					1629:1632	the CaSiO3 @Ca2 SiO4	1613:1632	the CaSiO3 @Ca2 SiO4	1613:1632	Extensive in vitro and in vivo evaluation showed that the CaSiO3 @Ca2 SiO4 and MBG@Ca2 SiO4 granules exhibited many superior properties such as controllable ion release, improved biodegradation and enhanced osteogenic capability in comparison with the pure Ca2 SiO4 @Ca2 SiO4 , thereby opening new mild-condition approach in fabricating osteogenesis-tailored silicate biomaterials for bone regenerative medicine, especially for efficient reconstruction of challenging pathological bone defects.
31037822	2	91	theme	pathological	549:560	arg1	bone					562:565	pathological bone	549:565	pathological bone	549:565	However, balancing the preparation conditions and biological performances of inorganic biomaterials remain a great challenge, especially when there is lack of biomaterial design on how to control component distribution and how pathological bone responds to the biomaterial stimulations and osteogenesis.
31037822	0	92	theme	femoral	118:124	arg1	defect					131:136	osteoporotic femoral bone defect	105:136	osteoporotic femoral bone defect	105:136	Composition control in biphasic silicate microspheres on stimulating new bone regeneration and repair of osteoporotic femoral bone defect.
31037822	7	93	theme	bone	2040:2043	arg1	defects					2045:2051	pathological bone defects	2027:2051	pathological bone defects	2027:2051	Extensive in vitro and in vivo evaluation showed that the CaSiO3 @Ca2 SiO4 and MBG@Ca2 SiO4 granules exhibited many superior properties such as controllable ion release, improved biodegradation and enhanced osteogenic capability in comparison with the pure Ca2 SiO4 @Ca2 SiO4 , thereby opening new mild-condition approach in fabricating osteogenesis-tailored silicate biomaterials for bone regenerative medicine, especially for efficient reconstruction of challenging pathological bone defects.
31037822	7	94	theme	osteogenic	1766:1775	arg1	capability					1777:1786	osteogenic capability	1766:1786	osteogenic capability	1766:1786	Extensive in vitro and in vivo evaluation showed that the CaSiO3 @Ca2 SiO4 and MBG@Ca2 SiO4 granules exhibited many superior properties such as controllable ion release, improved biodegradation and enhanced osteogenic capability in comparison with the pure Ca2 SiO4 @Ca2 SiO4 , thereby opening new mild-condition approach in fabricating osteogenesis-tailored silicate biomaterials for bone regenerative medicine, especially for efficient reconstruction of challenging pathological bone defects.
31037822	7	95	from	approach	1872:1879	arg1	biomaterials					1927:1938	fabricating osteogenesis-tailored silicate biomaterials	1884:1938	fabricating osteogenesis-tailored silicate biomaterials	1884:1938	Extensive in vitro and in vivo evaluation showed that the CaSiO3 @Ca2 SiO4 and MBG@Ca2 SiO4 granules exhibited many superior properties such as controllable ion release, improved biodegradation and enhanced osteogenic capability in comparison with the pure Ca2 SiO4 @Ca2 SiO4 , thereby opening new mild-condition approach in fabricating osteogenesis-tailored silicate biomaterials for bone regenerative medicine, especially for efficient reconstruction of challenging pathological bone defects.
31037822	4	96	theme	CaSiO3	997:1002	arg1	SiO4					1024:1027	MBG@Ca2 SiO4	1016:1027	MBG@Ca2 SiO4	1016:1027	The introduction of β-calcium silicate (CaSiO3 ) or mesoporous bioactive glass (MBG) into self-curing β-dicalcium silicate (Ca2 SiO4 ) cement shell to form spherical granules (CaSiO3 @Ca2 SiO4 , MBG@Ca2 SiO4 ) was to retain the physicochemical property and/or microstructure of each component for optimizing bioactive ion release that could maximize osteostimulation in osteoporosis.
31037822	4	96	theme	CaSiO3	997:1002	arg1	SiO4					1009:1012	CaSiO3 @Ca2 SiO4	997:1012	CaSiO3 @Ca2 SiO4	997:1012	The introduction of β-calcium silicate (CaSiO3 ) or mesoporous bioactive glass (MBG) into self-curing β-dicalcium silicate (Ca2 SiO4 ) cement shell to form spherical granules (CaSiO3 @Ca2 SiO4 , MBG@Ca2 SiO4 ) was to retain the physicochemical property and/or microstructure of each component for optimizing bioactive ion release that could maximize osteostimulation in osteoporosis.
31037822	6	97	theme	inorganic	1514:1522	arg1	powders					1524:1530	inorganic powders	1514:1530	inorganic powders	1514:1530	This unique heterostructure preparation is governed by coaxially aligned bilayer nozzle, inorganic powders and biocompatible binders.
31037822	2	98	theme	biomaterials	409:420	arg1	performances					383:394	biological performances	372:394	biological performances of inorganic biomaterials	372:420	However, balancing the preparation conditions and biological performances of inorganic biomaterials remain a great challenge, especially when there is lack of biomaterial design on how to control component distribution and how pathological bone responds to the biomaterial stimulations and osteogenesis.
31037822	0	99	theme	biphasic	23:30	arg1	microspheres					41:52	biphasic silicate microspheres	23:52	biphasic silicate microspheres	23:52	Composition control in biphasic silicate microspheres on stimulating new bone regeneration and repair of osteoporotic femoral bone defect.
31037822	1	100	theme	challengeable	294:306	arg1	defects					313:319	challengeable bone defects	294:319	challengeable bone defects	294:319	Application of bioactive materials as synthetic bone graft substitutes in regenerative medicine has seen great evolution over the past decades in treating challengeable bone defects.
31037822	5	101	theme	macropore	1365:1373	arg1	networks					1375:1382	interconnected macropore networks	1350:1382	interconnected macropore networks	1350:1382	We report a scalable shape-controlled mild fabrication protocol to yield the yolk-shell granules, endowing to different phases in yolk layer and interconnected macropore networks in the closely packed granule scaffolds.
31037822	6	102	theme	aligned	1490:1496	arg1	nozzle					1506:1511	coaxially aligned bilayer nozzle	1480:1511	coaxially aligned bilayer nozzle	1480:1511	This unique heterostructure preparation is governed by coaxially aligned bilayer nozzle, inorganic powders and biocompatible binders.
31037822	5	103	theme	shape-controlled	1226:1241	arg1	protocol					1260:1267	a scalable shape-controlled mild fabrication protocol	1215:1267	a scalable shape-controlled mild fabrication protocol to yield the yolk-shell granules, endowing to different phases in yolk layer and interconnected macropore networks in the closely packed granule scaffolds	1215:1422	We report a scalable shape-controlled mild fabrication protocol to yield the yolk-shell granules, endowing to different phases in yolk layer and interconnected macropore networks in the closely packed granule scaffolds.
31037822	7	104	theme	CaSiO3	1617:1622	arg1	SiO4					1629:1632	the CaSiO3 @Ca2 SiO4	1613:1632	the CaSiO3 @Ca2 SiO4	1613:1632	Extensive in vitro and in vivo evaluation showed that the CaSiO3 @Ca2 SiO4 and MBG@Ca2 SiO4 granules exhibited many superior properties such as controllable ion release, improved biodegradation and enhanced osteogenic capability in comparison with the pure Ca2 SiO4 @Ca2 SiO4 , thereby opening new mild-condition approach in fabricating osteogenesis-tailored silicate biomaterials for bone regenerative medicine, especially for efficient reconstruction of challenging pathological bone defects.
31037822	5	105	theme	fabrication	1248:1258	arg1	protocol					1260:1267	a scalable shape-controlled mild fabrication protocol	1215:1267	a scalable shape-controlled mild fabrication protocol to yield the yolk-shell granules, endowing to different phases in yolk layer and interconnected macropore networks in the closely packed granule scaffolds	1215:1422	We report a scalable shape-controlled mild fabrication protocol to yield the yolk-shell granules, endowing to different phases in yolk layer and interconnected macropore networks in the closely packed granule scaffolds.
31037822	4	106	theme	@	1019:1019	arg1	SiO4					1024:1027	MBG@Ca2 SiO4	1016:1027	MBG@Ca2 SiO4	1016:1027	The introduction of β-calcium silicate (CaSiO3 ) or mesoporous bioactive glass (MBG) into self-curing β-dicalcium silicate (Ca2 SiO4 ) cement shell to form spherical granules (CaSiO3 @Ca2 SiO4 , MBG@Ca2 SiO4 ) was to retain the physicochemical property and/or microstructure of each component for optimizing bioactive ion release that could maximize osteostimulation in osteoporosis.
31037822	4	106	theme	@	1019:1019	arg1	SiO4					1009:1012	CaSiO3 @Ca2 SiO4	997:1012	CaSiO3 @Ca2 SiO4	997:1012	The introduction of β-calcium silicate (CaSiO3 ) or mesoporous bioactive glass (MBG) into self-curing β-dicalcium silicate (Ca2 SiO4 ) cement shell to form spherical granules (CaSiO3 @Ca2 SiO4 , MBG@Ca2 SiO4 ) was to retain the physicochemical property and/or microstructure of each component for optimizing bioactive ion release that could maximize osteostimulation in osteoporosis.
31037822	3	107	theme	Ca-silicate	671:681	arg1	microspheres					683:694	yolk-shell Ca-silicate microspheres	660:694	yolk-shell Ca-silicate microspheres	660:694	Here, our objective is to develop yolk-shell Ca-silicate microspheres and to investigate the potential biological performances to overcome the limitations in repair of osteoporotic bone defects.
31037822	7	108	theme	bone	1944:1947	arg1	medicine					1962:1969	bone regenerative medicine	1944:1969	bone regenerative medicine	1944:1969	Extensive in vitro and in vivo evaluation showed that the CaSiO3 @Ca2 SiO4 and MBG@Ca2 SiO4 granules exhibited many superior properties such as controllable ion release, improved biodegradation and enhanced osteogenic capability in comparison with the pure Ca2 SiO4 @Ca2 SiO4 , thereby opening new mild-condition approach in fabricating osteogenesis-tailored silicate biomaterials for bone regenerative medicine, especially for efficient reconstruction of challenging pathological bone defects.
31037822	2	109	theme	biomaterial	481:491	arg1	design					493:498	biomaterial design	481:498	biomaterial design	481:498	However, balancing the preparation conditions and biological performances of inorganic biomaterials remain a great challenge, especially when there is lack of biomaterial design on how to control component distribution and how pathological bone responds to the biomaterial stimulations and osteogenesis.
31037822	3	110	theme	potential	719:727	arg1	performances					740:751	the potential biological performances	715:751	the potential biological performances	715:751	Here, our objective is to develop yolk-shell Ca-silicate microspheres and to investigate the potential biological performances to overcome the limitations in repair of osteoporotic bone defects.
31037822	4	111	theme	bioactive	884:892	arg1	MBG					901:903	MBG	901:903	MBG	901:903	The introduction of β-calcium silicate (CaSiO3 ) or mesoporous bioactive glass (MBG) into self-curing β-dicalcium silicate (Ca2 SiO4 ) cement shell to form spherical granules (CaSiO3 @Ca2 SiO4 , MBG@Ca2 SiO4 ) was to retain the physicochemical property and/or microstructure of each component for optimizing bioactive ion release that could maximize osteostimulation in osteoporosis.
31037822	4	111	theme	bioactive	884:892	arg1	glass					894:898	mesoporous bioactive glass	873:898	mesoporous bioactive glass (MBG)	873:904	The introduction of β-calcium silicate (CaSiO3 ) or mesoporous bioactive glass (MBG) into self-curing β-dicalcium silicate (Ca2 SiO4 ) cement shell to form spherical granules (CaSiO3 @Ca2 SiO4 , MBG@Ca2 SiO4 ) was to retain the physicochemical property and/or microstructure of each component for optimizing bioactive ion release that could maximize osteostimulation in osteoporosis.
31037822	7	112	theme	regenerative	1949:1960	arg1	medicine					1962:1969	bone regenerative medicine	1944:1969	bone regenerative medicine	1944:1969	Extensive in vitro and in vivo evaluation showed that the CaSiO3 @Ca2 SiO4 and MBG@Ca2 SiO4 granules exhibited many superior properties such as controllable ion release, improved biodegradation and enhanced osteogenic capability in comparison with the pure Ca2 SiO4 @Ca2 SiO4 , thereby opening new mild-condition approach in fabricating osteogenesis-tailored silicate biomaterials for bone regenerative medicine, especially for efficient reconstruction of challenging pathological bone defects.
31037822	4	113	theme	silicate	851:858	arg1	introduction					825:836	The introduction	821:836	The introduction of β-calcium silicate (CaSiO3 ) or mesoporous bioactive glass (MBG) into self-curing β-dicalcium silicate (Ca2 SiO4 ) cement shell to form spherical granules (CaSiO3 @Ca2 SiO4 , MBG@Ca2 SiO4 )	821:1029	The introduction of β-calcium silicate (CaSiO3 ) or mesoporous bioactive glass (MBG) into self-curing β-dicalcium silicate (Ca2 SiO4 ) cement shell to form spherical granules (CaSiO3 @Ca2 SiO4 , MBG@Ca2 SiO4 ) was to retain the physicochemical property and/or microstructure of each component for optimizing bioactive ion release that could maximize osteostimulation in osteoporosis.
31037822	5	114	theme	granule	1406:1412	arg1	scaffolds					1414:1422	the closely packed granule scaffolds	1387:1422	the closely packed granule scaffolds	1387:1422	We report a scalable shape-controlled mild fabrication protocol to yield the yolk-shell granules, endowing to different phases in yolk layer and interconnected macropore networks in the closely packed granule scaffolds.
31037822	1	115	theme	great	244:248	arg1	evolution					250:258	great evolution	244:258	great evolution over the past decades	244:280	Application of bioactive materials as synthetic bone graft substitutes in regenerative medicine has seen great evolution over the past decades in treating challengeable bone defects.
31037822	7	116	theme	in	1582:1583	arg1	evaluation					1590:1599	Extensive in vitro and in vivo evaluation	1559:1599	Extensive in vitro and in vivo evaluation	1559:1599	Extensive in vitro and in vivo evaluation showed that the CaSiO3 @Ca2 SiO4 and MBG@Ca2 SiO4 granules exhibited many superior properties such as controllable ion release, improved biodegradation and enhanced osteogenic capability in comparison with the pure Ca2 SiO4 @Ca2 SiO4 , thereby opening new mild-condition approach in fabricating osteogenesis-tailored silicate biomaterials for bone regenerative medicine, especially for efficient reconstruction of challenging pathological bone defects.
31037822	7	117	theme	osteogenesis-tailored	1896:1916	arg1	biomaterials					1927:1938	fabricating osteogenesis-tailored silicate biomaterials	1884:1938	fabricating osteogenesis-tailored silicate biomaterials	1884:1938	Extensive in vitro and in vivo evaluation showed that the CaSiO3 @Ca2 SiO4 and MBG@Ca2 SiO4 granules exhibited many superior properties such as controllable ion release, improved biodegradation and enhanced osteogenic capability in comparison with the pure Ca2 SiO4 @Ca2 SiO4 , thereby opening new mild-condition approach in fabricating osteogenesis-tailored silicate biomaterials for bone regenerative medicine, especially for efficient reconstruction of challenging pathological bone defects.
31037822	4	118	theme	component	1104:1112	arg1	property					1065:1072	the physicochemical property	1045:1072	the physicochemical property	1045:1072	The introduction of β-calcium silicate (CaSiO3 ) or mesoporous bioactive glass (MBG) into self-curing β-dicalcium silicate (Ca2 SiO4 ) cement shell to form spherical granules (CaSiO3 @Ca2 SiO4 , MBG@Ca2 SiO4 ) was to retain the physicochemical property and/or microstructure of each component for optimizing bioactive ion release that could maximize osteostimulation in osteoporosis.
34354519	9	0	theme	glycans	1378:1384	arg1	bioconjugation					1360:1373	the bioconjugation	1356:1373	the bioconjugation of glycans by using click chemistry	1356:1409	Because the glycosyl azide is applicable in the bioconjugation of glycans by using click chemistry, the enzyme-assisted production of the glycosyl azide may contribute to the field of glycobiology.
34354519	5	1	theme	catalytic	672:680	arg1	dyad					682:685	a catalytic dyad	670:685	a catalytic dyad with enhanced stability	670:709	In the present study, we engineered a catalytic dyad with enhanced stability by site-directed mutagenesis and crystallographic study of N127S/E128H.
34354519	8	2	from	presence	1145:1152	arg1	complex					1205:1211	the Michaelis complex	1191:1211	the Michaelis complex of the triple mutant, which forms the productive tautomer of His128 that acts as an acid catalyst	1191:1309	We confirmed the presence of a stable Ser-His hydrogen bond in the Michaelis complex of the triple mutant, which forms the productive tautomer of His128 that acts as an acid catalyst.
34354519	9	3	theme	glycosyl	1324:1331	arg1	azide					1333:1337	the glycosyl azide	1320:1337	the glycosyl azide	1320:1337	Because the glycosyl azide is applicable in the bioconjugation of glycans by using click chemistry, the enzyme-assisted production of the glycosyl azide may contribute to the field of glycobiology.
34354519	9	3	theme	glycosyl	1324:1331	arg1	applicable					1342:1351	applicable	1342:1351	applicable	1342:1351	Because the glycosyl azide is applicable in the bioconjugation of glycans by using click chemistry, the enzyme-assisted production of the glycosyl azide may contribute to the field of glycobiology.
34354519	4	4	theme	azidolysis	613:622	arg1	reaction					624:631	the azidolysis reaction	609:631	the azidolysis reaction	609:631	A crystallographic study reported that the transient formation of a Ser-His catalytic dyad in the reaction cycle possibly reduced the azidolysis reaction.
34354519	0	5	theme	Chemical	67:74	arg1	Properties					76:85	Soil Chemical Properties	62:85	Soil Chemical Properties	62:85	Sugar Composition in Asparagus Spears and Its Relationship to Soil Chemical Properties.
34354519	4	6	theme	Ser-His	547:553	arg1	dyad					565:568	a Ser-His catalytic dyad	545:568	a Ser-His catalytic dyad	545:568	A crystallographic study reported that the transient formation of a Ser-His catalytic dyad in the reaction cycle possibly reduced the azidolysis reaction.
34354519	5	7	theme	enhanced	692:699	arg1	stability					701:709	enhanced stability	692:709	enhanced stability	692:709	In the present study, we engineered a catalytic dyad with enhanced stability by site-directed mutagenesis and crystallographic study of N127S/E128H.
34354519	2	8	contain	possesses	269:277	arg1	xylanase					178:185	A retaining xylanase	166:185	A retaining xylanase from Streptomyces olivaceoviridis E-86 belonging to glycoside hydrolase family 10	166:267	A retaining xylanase from Streptomyces olivaceoviridis E-86 belonging to glycoside hydrolase family 10 possesses Glu128 and Glu236 that respectively function as acid/base and nucleophile.
34354519	2	8	contain	possesses	269:277	arg2	Glu236					290:295	Glu236	290:295	Glu236	290:295	A retaining xylanase from Streptomyces olivaceoviridis E-86 belonging to glycoside hydrolase family 10 possesses Glu128 and Glu236 that respectively function as acid/base and nucleophile.
34354519	2	8	contain	possesses	269:277	arg2	Glu128					279:284	Glu128	279:284	Glu128	279:284	A retaining xylanase from Streptomyces olivaceoviridis E-86 belonging to glycoside hydrolase family 10 possesses Glu128 and Glu236 that respectively function as acid/base and nucleophile.
34354519	5	9	theme	N127S/E128H	770:780	arg1	mutagenesis					728:738	site-directed mutagenesis	714:738	site-directed mutagenesis	714:738	In the present study, we engineered a catalytic dyad with enhanced stability by site-directed mutagenesis and crystallographic study of N127S/E128H.
34354519	5	9	theme	N127S/E128H	770:780	arg1	study					761:765	crystallographic study	744:765	crystallographic study	744:765	In the present study, we engineered a catalytic dyad with enhanced stability by site-directed mutagenesis and crystallographic study of N127S/E128H.
34354519	7	10	theme	T82A	1021:1024	arg1	mutation					1026:1033	T82A mutation	1021:1033	T82A mutation	1021:1033	The introduction of T82A mutation in N127S/E128H produces an enhanced first-order rate constant (6 times that of N127S/E128H).
34354519	0	11	from	Relationship	46:57	arg1	Spears					31:36	Asparagus Spears	21:36	Asparagus Spears	21:36	Sugar Composition in Asparagus Spears and Its Relationship to Soil Chemical Properties.
34354519	4	12	from	formation	532:540	arg1	cycle					586:590	the reaction cycle	573:590	the reaction cycle	573:590	A crystallographic study reported that the transient formation of a Ser-His catalytic dyad in the reaction cycle possibly reduced the azidolysis reaction.
34354519	5	13	theme	present	641:647	arg1	study					649:653	the present study	637:653	the present study	637:653	In the present study, we engineered a catalytic dyad with enhanced stability by site-directed mutagenesis and crystallographic study of N127S/E128H.
34354519	8	14	theme	triple	1220:1225	arg1	mutant					1227:1232	the triple mutant	1216:1232	the triple mutant	1216:1232	We confirmed the presence of a stable Ser-His hydrogen bond in the Michaelis complex of the triple mutant, which forms the productive tautomer of His128 that acts as an acid catalyst.
34354519	9	15	from	bioconjugation	1360:1373	arg1	azide					1333:1337	the glycosyl azide	1320:1337	the glycosyl azide	1320:1337	Because the glycosyl azide is applicable in the bioconjugation of glycans by using click chemistry, the enzyme-assisted production of the glycosyl azide may contribute to the field of glycobiology.
34354519	9	15	from	bioconjugation	1360:1373	arg1	applicable					1342:1351	applicable	1342:1351	applicable	1342:1351	Because the glycosyl azide is applicable in the bioconjugation of glycans by using click chemistry, the enzyme-assisted production of the glycosyl azide may contribute to the field of glycobiology.
34354519	9	16	theme	glycosyl	1450:1457	arg1	azide					1459:1463	the glycosyl azide	1446:1463	the glycosyl azide	1446:1463	Because the glycosyl azide is applicable in the bioconjugation of glycans by using click chemistry, the enzyme-assisted production of the glycosyl azide may contribute to the field of glycobiology.
34354519	6	17	with	Comparison	783:792	arg1	pNP-X2					841:846	pNP-X2	841:846	pNP-X2	841:846	Comparison of the Michaelis complexes of N127S/E128H with pNP-X2 and with xylopentaose showed that Ser127 could form an alternative hydrogen bond with Thr82, which disrupts the formation of the Ser-His catalytic dyad.
34354519	6	17	with	Comparison	783:792	arg1	xylopentaose					857:868	xylopentaose	857:868	xylopentaose	857:868	Comparison of the Michaelis complexes of N127S/E128H with pNP-X2 and with xylopentaose showed that Ser127 could form an alternative hydrogen bond with Thr82, which disrupts the formation of the Ser-His catalytic dyad.
34354519	7	18	dep	constant	1088:1095	arg1	times					1100:1104	6 times	1098:1104	6 times that of N127S/E128H	1098:1124	The introduction of T82A mutation in N127S/E128H produces an enhanced first-order rate constant (6 times that of N127S/E128H).
34354519	2	19	from	E-86	221:224	arg1	xylanase					178:185	A retaining xylanase	166:185	A retaining xylanase from Streptomyces olivaceoviridis E-86 belonging to glycoside hydrolase family 10	166:267	A retaining xylanase from Streptomyces olivaceoviridis E-86 belonging to glycoside hydrolase family 10 possesses Glu128 and Glu236 that respectively function as acid/base and nucleophile.
34354519	4	20	theme	transient	522:530	arg1	formation					532:540	the transient formation	518:540	the transient formation of a Ser-His catalytic dyad in the reaction cycle	518:590	A crystallographic study reported that the transient formation of a Ser-His catalytic dyad in the reaction cycle possibly reduced the azidolysis reaction.
34354519	6	21	theme	alternative	903:913	arg1	bond					924:927	an alternative hydrogen bond	900:927	an alternative hydrogen bond with Thr82, which disrupts the formation of the Ser-His catalytic dyad	900:998	Comparison of the Michaelis complexes of N127S/E128H with pNP-X2 and with xylopentaose showed that Ser127 could form an alternative hydrogen bond with Thr82, which disrupts the formation of the Ser-His catalytic dyad.
34354519	0	22	theme	Sugar	0:4	arg1	Composition					6:16	Sugar Composition	0:16	Sugar Composition in Asparagus Spears	0:36	Sugar Composition in Asparagus Spears and Its Relationship to Soil Chemical Properties.
34354519	7	23	theme	constant	1088:1095	arg1	rate					1083:1086	an enhanced first-order rate	1059:1086	an enhanced first-order rate constant (6 times that of N127S/E128H)	1059:1125	The introduction of T82A mutation in N127S/E128H produces an enhanced first-order rate constant (6 times that of N127S/E128H).
34354519	3	24	theme	xylanase	411:418	arg1	mutant					387:392	a unique mutant	378:392	a unique mutant of the retaining xylanase	378:418	We previously developed a unique mutant of the retaining xylanase, N127S/E128H, whose deglycosylation is triggered by azide.
34354519	3	24	theme	xylanase	411:418	arg1	N127S/E128H					421:431	N127S/E128H	421:431	N127S/E128H	421:431	We previously developed a unique mutant of the retaining xylanase, N127S/E128H, whose deglycosylation is triggered by azide.
34354519	8	25	theme	bond	1183:1186	arg1	presence					1145:1152	the presence	1141:1152	the presence of a stable Ser-His hydrogen bond in the Michaelis complex of the triple mutant, which forms the productive tautomer of His128 that acts as an acid catalyst	1141:1309	We confirmed the presence of a stable Ser-His hydrogen bond in the Michaelis complex of the triple mutant, which forms the productive tautomer of His128 that acts as an acid catalyst.
34354519	8	26	theme	hydrogen	1174:1181	arg1	bond					1183:1186	a stable Ser-His hydrogen bond	1157:1186	a stable Ser-His hydrogen bond in the Michaelis complex of the triple mutant, which forms the productive tautomer of His128 that acts as an acid catalyst	1157:1309	We confirmed the presence of a stable Ser-His hydrogen bond in the Michaelis complex of the triple mutant, which forms the productive tautomer of His128 that acts as an acid catalyst.
34354519	0	27	from	Composition	6:16	arg1	Spears					31:36	Asparagus Spears	21:36	Asparagus Spears	21:36	Sugar Composition in Asparagus Spears and Its Relationship to Soil Chemical Properties.
34354519	1	28	theme	Glycoside	88:96	arg1	hydrolases					98:107	Glycoside hydrolases	88:107	Glycoside hydrolases	88:107	Glycoside hydrolases require carboxyl groups as catalysts for their activity.
34354519	6	29	with	bond	924:927	arg1	Thr82					934:938	Thr82	934:938	Thr82	934:938	Comparison of the Michaelis complexes of N127S/E128H with pNP-X2 and with xylopentaose showed that Ser127 could form an alternative hydrogen bond with Thr82, which disrupts the formation of the Ser-His catalytic dyad.
34354519	0	30	theme	Asparagus	21:29	arg1	Spears					31:36	Asparagus Spears	21:36	Asparagus Spears	21:36	Sugar Composition in Asparagus Spears and Its Relationship to Soil Chemical Properties.
34354519	4	31	theme	reaction	577:584	arg1	cycle					586:590	the reaction cycle	573:590	the reaction cycle	573:590	A crystallographic study reported that the transient formation of a Ser-His catalytic dyad in the reaction cycle possibly reduced the azidolysis reaction.
34354519	7	32	theme	N127S/E128H	1114:1124	arg1	times					1100:1104	6 times	1098:1104	6 times that of N127S/E128H	1098:1124	The introduction of T82A mutation in N127S/E128H produces an enhanced first-order rate constant (6 times that of N127S/E128H).
34354519	6	33	theme	N127S/E128H	824:834	arg1	complexes					811:819	the Michaelis complexes	797:819	the Michaelis complexes of N127S/E128H	797:834	Comparison of the Michaelis complexes of N127S/E128H with pNP-X2 and with xylopentaose showed that Ser127 could form an alternative hydrogen bond with Thr82, which disrupts the formation of the Ser-His catalytic dyad.
34354519	3	34	theme	retaining	401:409	arg1	xylanase					411:418	the retaining xylanase	397:418	the retaining xylanase	397:418	We previously developed a unique mutant of the retaining xylanase, N127S/E128H, whose deglycosylation is triggered by azide.
34354519	5	35	theme	crystallographic	744:759	arg1	study					761:765	crystallographic study	744:765	crystallographic study	744:765	In the present study, we engineered a catalytic dyad with enhanced stability by site-directed mutagenesis and crystallographic study of N127S/E128H.
34354519	5	36	with	dyad	682:685	arg1	stability					701:709	enhanced stability	692:709	enhanced stability	692:709	In the present study, we engineered a catalytic dyad with enhanced stability by site-directed mutagenesis and crystallographic study of N127S/E128H.
34354519	8	37	from	bond	1183:1186	arg1	complex					1205:1211	the Michaelis complex	1191:1211	the Michaelis complex of the triple mutant, which forms the productive tautomer of His128 that acts as an acid catalyst	1191:1309	We confirmed the presence of a stable Ser-His hydrogen bond in the Michaelis complex of the triple mutant, which forms the productive tautomer of His128 that acts as an acid catalyst.
34354519	8	38	theme	His128	1274:1279	arg1	tautomer					1262:1269	the productive tautomer	1247:1269	the productive tautomer of His128 that acts as an acid catalyst	1247:1309	We confirmed the presence of a stable Ser-His hydrogen bond in the Michaelis complex of the triple mutant, which forms the productive tautomer of His128 that acts as an acid catalyst.
34354519	8	38	theme	His128	1274:1279	arg1	catalyst					1302:1309	an acid catalyst	1294:1309	an acid catalyst	1294:1309	We confirmed the presence of a stable Ser-His hydrogen bond in the Michaelis complex of the triple mutant, which forms the productive tautomer of His128 that acts as an acid catalyst.
34354519	8	39	theme	acid	1297:1300	arg1	catalyst					1302:1309	an acid catalyst	1294:1309	an acid catalyst	1294:1309	We confirmed the presence of a stable Ser-His hydrogen bond in the Michaelis complex of the triple mutant, which forms the productive tautomer of His128 that acts as an acid catalyst.
34354519	8	39	theme	acid	1297:1300	arg1	tautomer					1262:1269	the productive tautomer	1247:1269	the productive tautomer of His128 that acts as an acid catalyst	1247:1309	We confirmed the presence of a stable Ser-His hydrogen bond in the Michaelis complex of the triple mutant, which forms the productive tautomer of His128 that acts as an acid catalyst.
34354519	8	40	theme	stable	1159:1164	arg1	bond					1183:1186	a stable Ser-His hydrogen bond	1157:1186	a stable Ser-His hydrogen bond in the Michaelis complex of the triple mutant, which forms the productive tautomer of His128 that acts as an acid catalyst	1157:1309	We confirmed the presence of a stable Ser-His hydrogen bond in the Michaelis complex of the triple mutant, which forms the productive tautomer of His128 that acts as an acid catalyst.
34354519	4	41	theme	dyad	565:568	arg1	formation					532:540	the transient formation	518:540	the transient formation of a Ser-His catalytic dyad in the reaction cycle	518:590	A crystallographic study reported that the transient formation of a Ser-His catalytic dyad in the reaction cycle possibly reduced the azidolysis reaction.
34354519	9	42	theme	click	1395:1399	arg1	chemistry					1401:1409	click chemistry	1395:1409	click chemistry	1395:1409	Because the glycosyl azide is applicable in the bioconjugation of glycans by using click chemistry, the enzyme-assisted production of the glycosyl azide may contribute to the field of glycobiology.
34354519	2	43	theme	retaining	168:176	arg1	xylanase					178:185	A retaining xylanase	166:185	A retaining xylanase from Streptomyces olivaceoviridis E-86 belonging to glycoside hydrolase family 10	166:267	A retaining xylanase from Streptomyces olivaceoviridis E-86 belonging to glycoside hydrolase family 10 possesses Glu128 and Glu236 that respectively function as acid/base and nucleophile.
34354519	2	44	theme	hydrolase	249:257	arg1	family					259:264	glycoside hydrolase family 10	239:267	glycoside hydrolase family 10	239:267	A retaining xylanase from Streptomyces olivaceoviridis E-86 belonging to glycoside hydrolase family 10 possesses Glu128 and Glu236 that respectively function as acid/base and nucleophile.
34354519	7	45	theme	first-order	1071:1081	arg1	rate					1083:1086	an enhanced first-order rate	1059:1086	an enhanced first-order rate constant (6 times that of N127S/E128H)	1059:1125	The introduction of T82A mutation in N127S/E128H produces an enhanced first-order rate constant (6 times that of N127S/E128H).
34354519	3	46	theme	unique	380:385	arg1	mutant					387:392	a unique mutant	378:392	a unique mutant of the retaining xylanase	378:418	We previously developed a unique mutant of the retaining xylanase, N127S/E128H, whose deglycosylation is triggered by azide.
34354519	3	46	theme	unique	380:385	arg1	N127S/E128H					421:431	N127S/E128H	421:431	N127S/E128H	421:431	We previously developed a unique mutant of the retaining xylanase, N127S/E128H, whose deglycosylation is triggered by azide.
34354519	8	47	theme	productive	1251:1260	arg1	tautomer					1262:1269	the productive tautomer	1247:1269	the productive tautomer of His128 that acts as an acid catalyst	1247:1309	We confirmed the presence of a stable Ser-His hydrogen bond in the Michaelis complex of the triple mutant, which forms the productive tautomer of His128 that acts as an acid catalyst.
34354519	8	47	theme	productive	1251:1260	arg1	catalyst					1302:1309	an acid catalyst	1294:1309	an acid catalyst	1294:1309	We confirmed the presence of a stable Ser-His hydrogen bond in the Michaelis complex of the triple mutant, which forms the productive tautomer of His128 that acts as an acid catalyst.
34354519	4	48	theme	catalytic	555:563	arg1	dyad					565:568	a Ser-His catalytic dyad	545:568	a Ser-His catalytic dyad	545:568	A crystallographic study reported that the transient formation of a Ser-His catalytic dyad in the reaction cycle possibly reduced the azidolysis reaction.
34354519	4	49	theme	crystallographic	481:496	arg1	study					498:502	A crystallographic study	479:502	A crystallographic study	479:502	A crystallographic study reported that the transient formation of a Ser-His catalytic dyad in the reaction cycle possibly reduced the azidolysis reaction.
34354519	2	50	theme	glycoside	239:247	arg1	family					259:264	glycoside hydrolase family 10	239:267	glycoside hydrolase family 10	239:267	A retaining xylanase from Streptomyces olivaceoviridis E-86 belonging to glycoside hydrolase family 10 possesses Glu128 and Glu236 that respectively function as acid/base and nucleophile.
34354519	6	51	theme	complexes	811:819	arg1	Comparison					783:792	Comparison	783:792	Comparison of the Michaelis complexes of N127S/E128H with pNP-X2 and with xylopentaose	783:868	Comparison of the Michaelis complexes of N127S/E128H with pNP-X2 and with xylopentaose showed that Ser127 could form an alternative hydrogen bond with Thr82, which disrupts the formation of the Ser-His catalytic dyad.
34354519	1	52	theme	carboxyl	117:124	arg1	catalysts					136:144	catalysts	136:144	catalysts for their activity	136:163	Glycoside hydrolases require carboxyl groups as catalysts for their activity.
34354519	1	52	theme	carboxyl	117:124	arg1	groups					126:131	carboxyl groups	117:131	carboxyl groups	117:131	Glycoside hydrolases require carboxyl groups as catalysts for their activity.
34354519	6	53	theme	catalytic	985:993	arg1	dyad					995:998	the Ser-His catalytic dyad	973:998	the Ser-His catalytic dyad	973:998	Comparison of the Michaelis complexes of N127S/E128H with pNP-X2 and with xylopentaose showed that Ser127 could form an alternative hydrogen bond with Thr82, which disrupts the formation of the Ser-His catalytic dyad.
34354519	9	54	theme	azide	1459:1463	arg1	production					1432:1441	the enzyme-assisted production	1412:1441	the enzyme-assisted production of the glycosyl azide	1412:1463	Because the glycosyl azide is applicable in the bioconjugation of glycans by using click chemistry, the enzyme-assisted production of the glycosyl azide may contribute to the field of glycobiology.
34354519	6	55	theme	Michaelis	801:809	arg1	complexes					811:819	the Michaelis complexes	797:819	the Michaelis complexes of N127S/E128H	797:834	Comparison of the Michaelis complexes of N127S/E128H with pNP-X2 and with xylopentaose showed that Ser127 could form an alternative hydrogen bond with Thr82, which disrupts the formation of the Ser-His catalytic dyad.
34354519	6	56	theme	hydrogen	915:922	arg1	bond					924:927	an alternative hydrogen bond	900:927	an alternative hydrogen bond with Thr82, which disrupts the formation of the Ser-His catalytic dyad	900:998	Comparison of the Michaelis complexes of N127S/E128H with pNP-X2 and with xylopentaose showed that Ser127 could form an alternative hydrogen bond with Thr82, which disrupts the formation of the Ser-His catalytic dyad.
34354519	6	57	theme	Ser-His	977:983	arg1	dyad					995:998	the Ser-His catalytic dyad	973:998	the Ser-His catalytic dyad	973:998	Comparison of the Michaelis complexes of N127S/E128H with pNP-X2 and with xylopentaose showed that Ser127 could form an alternative hydrogen bond with Thr82, which disrupts the formation of the Ser-His catalytic dyad.
34354519	5	58	theme	site-directed	714:726	arg1	mutagenesis					728:738	site-directed mutagenesis	714:738	site-directed mutagenesis	714:738	In the present study, we engineered a catalytic dyad with enhanced stability by site-directed mutagenesis and crystallographic study of N127S/E128H.
34354519	8	59	from	complex	1205:1211	arg1	presence					1145:1152	the presence	1141:1152	the presence of a stable Ser-His hydrogen bond in the Michaelis complex of the triple mutant, which forms the productive tautomer of His128 that acts as an acid catalyst	1141:1309	We confirmed the presence of a stable Ser-His hydrogen bond in the Michaelis complex of the triple mutant, which forms the productive tautomer of His128 that acts as an acid catalyst.
34354519	7	60	theme	enhanced	1062:1069	arg1	rate					1083:1086	an enhanced first-order rate	1059:1086	an enhanced first-order rate constant (6 times that of N127S/E128H)	1059:1125	The introduction of T82A mutation in N127S/E128H produces an enhanced first-order rate constant (6 times that of N127S/E128H).
34354519	8	61	theme	Michaelis	1195:1203	arg1	complex					1205:1211	the Michaelis complex	1191:1211	the Michaelis complex of the triple mutant, which forms the productive tautomer of His128 that acts as an acid catalyst	1191:1309	We confirmed the presence of a stable Ser-His hydrogen bond in the Michaelis complex of the triple mutant, which forms the productive tautomer of His128 that acts as an acid catalyst.
34354519	2	62	theme	olivaceoviridis	205:219	arg1	E-86					221:224	Streptomyces olivaceoviridis E-86	192:224	Streptomyces olivaceoviridis E-86 belonging to glycoside hydrolase family 10	192:267	A retaining xylanase from Streptomyces olivaceoviridis E-86 belonging to glycoside hydrolase family 10 possesses Glu128 and Glu236 that respectively function as acid/base and nucleophile.
34354519	9	63	from	applicable	1342:1351	arg1	bioconjugation					1360:1373	the bioconjugation	1356:1373	the bioconjugation of glycans by using click chemistry	1356:1409	Because the glycosyl azide is applicable in the bioconjugation of glycans by using click chemistry, the enzyme-assisted production of the glycosyl azide may contribute to the field of glycobiology.
34354519	7	64	dep	times	1100:1104	arg1	that					1106:1109	that	1106:1109	that	1106:1109	The introduction of T82A mutation in N127S/E128H produces an enhanced first-order rate constant (6 times that of N127S/E128H).
34354519	0	65	theme	Soil	62:65	arg1	Properties					76:85	Soil Chemical Properties	62:85	Soil Chemical Properties	62:85	Sugar Composition in Asparagus Spears and Its Relationship to Soil Chemical Properties.
34354519	2	66	theme	Streptomyces	192:203	arg1	E-86					221:224	Streptomyces olivaceoviridis E-86	192:224	Streptomyces olivaceoviridis E-86 belonging to glycoside hydrolase family 10	192:267	A retaining xylanase from Streptomyces olivaceoviridis E-86 belonging to glycoside hydrolase family 10 possesses Glu128 and Glu236 that respectively function as acid/base and nucleophile.
34354519	9	67	theme	glycobiology	1496:1507	arg1	field					1487:1491	the field	1483:1491	the field of glycobiology	1483:1507	Because the glycosyl azide is applicable in the bioconjugation of glycans by using click chemistry, the enzyme-assisted production of the glycosyl azide may contribute to the field of glycobiology.
34354519	8	68	theme	Ser-His	1166:1172	arg1	bond					1183:1186	a stable Ser-His hydrogen bond	1157:1186	a stable Ser-His hydrogen bond in the Michaelis complex of the triple mutant, which forms the productive tautomer of His128 that acts as an acid catalyst	1157:1309	We confirmed the presence of a stable Ser-His hydrogen bond in the Michaelis complex of the triple mutant, which forms the productive tautomer of His128 that acts as an acid catalyst.
34354519	8	69	theme	mutant	1227:1232	arg1	complex					1205:1211	the Michaelis complex	1191:1211	the Michaelis complex of the triple mutant, which forms the productive tautomer of His128 that acts as an acid catalyst	1191:1309	We confirmed the presence of a stable Ser-His hydrogen bond in the Michaelis complex of the triple mutant, which forms the productive tautomer of His128 that acts as an acid catalyst.
34354519	7	70	theme	mutation	1026:1033	arg1	introduction					1005:1016	The introduction	1001:1016	The introduction of T82A mutation in N127S/E128H	1001:1048	The introduction of T82A mutation in N127S/E128H produces an enhanced first-order rate constant (6 times that of N127S/E128H).
34354519	7	71	from	introduction	1005:1016	arg1	N127S/E128H					1038:1048	N127S/E128H	1038:1048	N127S/E128H	1038:1048	The introduction of T82A mutation in N127S/E128H produces an enhanced first-order rate constant (6 times that of N127S/E128H).
34354519	9	72	theme	enzyme-assisted	1416:1430	arg1	production					1432:1441	the enzyme-assisted production	1412:1441	the enzyme-assisted production of the glycosyl azide	1412:1463	Because the glycosyl azide is applicable in the bioconjugation of glycans by using click chemistry, the enzyme-assisted production of the glycosyl azide may contribute to the field of glycobiology.
34354519	6	73	theme	dyad	995:998	arg1	formation					960:968	the formation	956:968	the formation of the Ser-His catalytic dyad	956:998	Comparison of the Michaelis complexes of N127S/E128H with pNP-X2 and with xylopentaose showed that Ser127 could form an alternative hydrogen bond with Thr82, which disrupts the formation of the Ser-His catalytic dyad.
30956301	7	0	theme	Hep-G2	971:976	arg1	cells					985:989	HeLa and Hep-G2 cancer cells	962:989	HeLa and Hep-G2 cancer cells	962:989	All the recovered fucoidans showed strong growth inhibition against HeLa and Hep-G2 cancer cells.
30956301	10	1	theme	fucoidan	1278:1285	arg1	structure					1287:1295	fucoidan structure	1278:1295	fucoidan structure influencing its anticancer and immunostimulatory activities	1278:1355	These results suggested that enzyme treatment preserved more sulfate groups in fucoidan structure influencing its anticancer and immunostimulatory activities.
30956301	10	2	theme	enzyme	1228:1233	arg1	treatment					1235:1243	enzyme treatment	1228:1243	enzyme treatment	1228:1243	These results suggested that enzyme treatment preserved more sulfate groups in fucoidan structure influencing its anticancer and immunostimulatory activities.
30956301	5	3	theme	fucoidans	754:762	arg1	weight					744:749	The weight mean average molecular weight	710:749	The weight mean average molecular weight of fucoidans	710:762	The weight mean average molecular weight of fucoidans varied between 443.7 and 1020.85 kDa.
30956301	6	4	theme	polysaccharide	806:819	arg1	chains					821:826	The polysaccharide chains	802:826	The polysaccharide chains	802:826	The polysaccharide chains were consisted of fucose, galactose, glucose, mannose and xylose.
30956301	4	5	theme	acids	690:694	arg1	levels					593:598	different levels	583:598	different levels of carbohydrates (52.78-58.65%), proteins (6.98-8.91%), sulfates (21.78-29.6%) and uronic acids (0.42-1.08%)	583:707	fucoidans were composed of different levels of carbohydrates (52.78-58.65%), proteins (6.98-8.91%), sulfates (21.78-29.6%) and uronic acids (0.42-1.08%).
30956301	3	6	theme	Enzyme-ultrasonic	412:428	arg1	fucoidan					439:446	Enzyme-ultrasonic isolated fucoidan	412:446	Enzyme-ultrasonic isolated fucoidan	412:446	Enzyme-ultrasonic isolated fucoidan showed the maximum extraction yield (7.87%) while that obtained by ultrasonic had the minimum value (3.6%).
30956301	7	7	theme	HeLa	962:965	arg1	cells					985:989	HeLa and Hep-G2 cancer cells	962:989	HeLa and Hep-G2 cancer cells	962:989	All the recovered fucoidans showed strong growth inhibition against HeLa and Hep-G2 cancer cells.
30956301	2	8	theme	chemical	318:325	arg1	activities					400:409	anticancer and immunomodulatory activities	368:409	anticancer and immunomodulatory activities	368:409	The extracted fucoidans were assessed for their chemical compositions, molecular characteristics, anticancer and immunomodulatory activities.
30956301	2	8	theme	chemical	318:325	arg1	compositions					327:338	their chemical compositions	312:338	their chemical compositions	312:338	The extracted fucoidans were assessed for their chemical compositions, molecular characteristics, anticancer and immunomodulatory activities.
30956301	2	8	theme	chemical	318:325	arg1	characteristics					351:365	molecular characteristics	341:365	molecular characteristics	341:365	The extracted fucoidans were assessed for their chemical compositions, molecular characteristics, anticancer and immunomodulatory activities.
30956301	3	9	theme	isolated	430:437	arg1	fucoidan					439:446	Enzyme-ultrasonic isolated fucoidan	412:446	Enzyme-ultrasonic isolated fucoidan	412:446	Enzyme-ultrasonic isolated fucoidan showed the maximum extraction yield (7.87%) while that obtained by ultrasonic had the minimum value (3.6%).
30956301	1	10	attach	isolated	168:175	arg2	polysaccharide					138:151	Sulfated polysaccharide	129:151	Sulfated polysaccharide (fucoidan)	129:162	Sulfated polysaccharide (fucoidan) was isolated from Nizamuddinia zanardinii by enzyme (alcalase), ultrasonic and enzyme-ultrasonic methods.
30956301	1	10	attach	isolated	168:175	arg2	fucoidan					154:161	fucoidan	154:161	fucoidan	154:161	Sulfated polysaccharide (fucoidan) was isolated from Nizamuddinia zanardinii by enzyme (alcalase), ultrasonic and enzyme-ultrasonic methods.
30956301	1	10	attach	isolated	168:175	arg1	Nizamuddinia					182:193	Nizamuddinia	182:193	Nizamuddinia	182:193	Sulfated polysaccharide (fucoidan) was isolated from Nizamuddinia zanardinii by enzyme (alcalase), ultrasonic and enzyme-ultrasonic methods.
30956301	7	11	theme	growth	936:941	arg1	inhibition					943:952	strong growth inhibition	929:952	strong growth inhibition	929:952	All the recovered fucoidans showed strong growth inhibition against HeLa and Hep-G2 cancer cells.
30956301	5	12	theme	mean	721:724	arg1	weight					744:749	The weight mean average molecular weight	710:749	The weight mean average molecular weight of fucoidans	710:762	The weight mean average molecular weight of fucoidans varied between 443.7 and 1020.85 kDa.
30956301	10	13	theme	anticancer	1313:1322	arg1	activities					1346:1355	its anticancer and immunostimulatory activities	1309:1355	its anticancer and immunostimulatory activities	1309:1355	These results suggested that enzyme treatment preserved more sulfate groups in fucoidan structure influencing its anticancer and immunostimulatory activities.
30956301	5	14	theme	average	726:732	arg1	weight					744:749	The weight mean average molecular weight	710:749	The weight mean average molecular weight of fucoidans	710:762	The weight mean average molecular weight of fucoidans varied between 443.7 and 1020.85 kDa.
30956301	7	15	theme	strong	929:934	arg1	inhibition					943:952	strong growth inhibition	929:952	strong growth inhibition	929:952	All the recovered fucoidans showed strong growth inhibition against HeLa and Hep-G2 cancer cells.
30956301	1	16	theme	enzyme	209:214	arg1	alcalase					217:224	enzyme (alcalase)	209:225	enzyme (alcalase)	209:225	Sulfated polysaccharide (fucoidan) was isolated from Nizamuddinia zanardinii by enzyme (alcalase), ultrasonic and enzyme-ultrasonic methods.
30956301	3	17	theme	maximum	459:465	arg1	yield					478:482	the maximum extraction yield	455:482	the maximum extraction yield (7.87%)	455:490	Enzyme-ultrasonic isolated fucoidan showed the maximum extraction yield (7.87%) while that obtained by ultrasonic had the minimum value (3.6%).
30956301	3	17	theme	maximum	459:465	arg1	%					489:489	7.87%	485:489	7.87%	485:489	Enzyme-ultrasonic isolated fucoidan showed the maximum extraction yield (7.87%) while that obtained by ultrasonic had the minimum value (3.6%).
30956301	8	18	theme	macrophage	1062:1071	arg1	cells					1073:1077	the macrophage cells	1058:1077	the macrophage cells	1058:1077	The isolated fucoidans were non-toxic and considerably stimulated the macrophage cells to release nitric oxide.
30956301	7	19	theme	recovered	902:910	arg1	fucoidans					912:920	All the recovered fucoidans	894:920	All the recovered fucoidans	894:920	All the recovered fucoidans showed strong growth inhibition against HeLa and Hep-G2 cancer cells.
30956301	2	20	theme	molecular	341:349	arg1	compositions					327:338	their chemical compositions	312:338	their chemical compositions	312:338	The extracted fucoidans were assessed for their chemical compositions, molecular characteristics, anticancer and immunomodulatory activities.
30956301	2	20	theme	molecular	341:349	arg1	characteristics					351:365	molecular characteristics	341:365	molecular characteristics	341:365	The extracted fucoidans were assessed for their chemical compositions, molecular characteristics, anticancer and immunomodulatory activities.
30956301	0	21	theme	zanardinii	30:39	arg1	polysaccharides					50:64	Nizamuddinia zanardinii sulfated polysaccharides	17:64	Nizamuddinia zanardinii sulfated polysaccharides	17:64	Bioactivities of Nizamuddinia zanardinii sulfated polysaccharides extracted by enzyme, ultrasound and enzyme-ultrasound methods.
30956301	4	22	theme	different	583:591	arg1	levels					593:598	different levels	583:598	different levels of carbohydrates (52.78-58.65%), proteins (6.98-8.91%), sulfates (21.78-29.6%) and uronic acids (0.42-1.08%)	583:707	fucoidans were composed of different levels of carbohydrates (52.78-58.65%), proteins (6.98-8.91%), sulfates (21.78-29.6%) and uronic acids (0.42-1.08%).
30956301	8	23	theme	isolated	996:1003	arg1	fucoidans					1005:1013	The isolated fucoidans	992:1013	The isolated fucoidans	992:1013	The isolated fucoidans were non-toxic and considerably stimulated the macrophage cells to release nitric oxide.
30956301	9	24	theme	Enzyme	1104:1109	arg1	extraction					1111:1120	Enzyme extraction	1104:1120	Enzyme extraction	1104:1120	Enzyme extraction produced fucoidan with the most macrophage stimulation capacity (> 42 µmol).
30956301	1	25	dep	Nizamuddinia	182:193	arg1	zanardinii					195:204	Nizamuddinia zanardinii	182:204	Nizamuddinia zanardinii	182:204	Sulfated polysaccharide (fucoidan) was isolated from Nizamuddinia zanardinii by enzyme (alcalase), ultrasonic and enzyme-ultrasonic methods.
30956301	4	26	theme	carbohydrates	603:615	arg1	levels					593:598	different levels	583:598	different levels of carbohydrates (52.78-58.65%), proteins (6.98-8.91%), sulfates (21.78-29.6%) and uronic acids (0.42-1.08%)	583:707	fucoidans were composed of different levels of carbohydrates (52.78-58.65%), proteins (6.98-8.91%), sulfates (21.78-29.6%) and uronic acids (0.42-1.08%).
30956301	1	27	theme	Sulfated	129:136	arg1	fucoidan					154:161	fucoidan	154:161	fucoidan	154:161	Sulfated polysaccharide (fucoidan) was isolated from Nizamuddinia zanardinii by enzyme (alcalase), ultrasonic and enzyme-ultrasonic methods.
30956301	1	27	theme	Sulfated	129:136	arg1	polysaccharide					138:151	Sulfated polysaccharide	129:151	Sulfated polysaccharide (fucoidan)	129:162	Sulfated polysaccharide (fucoidan) was isolated from Nizamuddinia zanardinii by enzyme (alcalase), ultrasonic and enzyme-ultrasonic methods.
30956301	0	28	theme	polysaccharides	50:64	arg1	Bioactivities					0:12	Bioactivities	0:12	Bioactivities of Nizamuddinia zanardinii sulfated polysaccharides	0:64	Bioactivities of Nizamuddinia zanardinii sulfated polysaccharides extracted by enzyme, ultrasound and enzyme-ultrasound methods.
30956301	10	29	theme	immunostimulatory	1328:1344	arg1	activities					1346:1355	its anticancer and immunostimulatory activities	1309:1355	its anticancer and immunostimulatory activities	1309:1355	These results suggested that enzyme treatment preserved more sulfate groups in fucoidan structure influencing its anticancer and immunostimulatory activities.
30956301	4	30	theme	sulfates	656:663	arg1	levels					593:598	different levels	583:598	different levels of carbohydrates (52.78-58.65%), proteins (6.98-8.91%), sulfates (21.78-29.6%) and uronic acids (0.42-1.08%)	583:707	fucoidans were composed of different levels of carbohydrates (52.78-58.65%), proteins (6.98-8.91%), sulfates (21.78-29.6%) and uronic acids (0.42-1.08%).
30956301	0	31	theme	sulfated	41:48	arg1	polysaccharides					50:64	Nizamuddinia zanardinii sulfated polysaccharides	17:64	Nizamuddinia zanardinii sulfated polysaccharides	17:64	Bioactivities of Nizamuddinia zanardinii sulfated polysaccharides extracted by enzyme, ultrasound and enzyme-ultrasound methods.
30956301	8	32	theme	nitric	1090:1095	arg1	oxide					1097:1101	nitric oxide	1090:1101	nitric oxide	1090:1101	The isolated fucoidans were non-toxic and considerably stimulated the macrophage cells to release nitric oxide.
30956301	3	33	contain	had	526:528	arg2	%					552:552	3.6%	549:552	3.6%	549:552	Enzyme-ultrasonic isolated fucoidan showed the maximum extraction yield (7.87%) while that obtained by ultrasonic had the minimum value (3.6%).
30956301	3	33	contain	had	526:528	arg1	that					498:501	that	498:501	that	498:501	Enzyme-ultrasonic isolated fucoidan showed the maximum extraction yield (7.87%) while that obtained by ultrasonic had the minimum value (3.6%).
30956301	3	33	contain	had	526:528	arg2	value					542:546	the minimum value	530:546	the minimum value (3.6%)	530:553	Enzyme-ultrasonic isolated fucoidan showed the maximum extraction yield (7.87%) while that obtained by ultrasonic had the minimum value (3.6%).
30956301	3	34	theme	extraction	467:476	arg1	yield					478:482	the maximum extraction yield	455:482	the maximum extraction yield (7.87%)	455:490	Enzyme-ultrasonic isolated fucoidan showed the maximum extraction yield (7.87%) while that obtained by ultrasonic had the minimum value (3.6%).
30956301	3	34	theme	extraction	467:476	arg1	%					489:489	7.87%	485:489	7.87%	485:489	Enzyme-ultrasonic isolated fucoidan showed the maximum extraction yield (7.87%) while that obtained by ultrasonic had the minimum value (3.6%).
30956301	2	35	theme	immunomodulatory	383:398	arg1	compositions					327:338	their chemical compositions	312:338	their chemical compositions	312:338	The extracted fucoidans were assessed for their chemical compositions, molecular characteristics, anticancer and immunomodulatory activities.
30956301	2	35	theme	immunomodulatory	383:398	arg1	activities					400:409	anticancer and immunomodulatory activities	368:409	anticancer and immunomodulatory activities	368:409	The extracted fucoidans were assessed for their chemical compositions, molecular characteristics, anticancer and immunomodulatory activities.
30956301	9	36	theme	macrophage	1154:1163	arg1	> 42 µmol					1187:1195	> 42 µmol	1187:1195	> 42 µmol	1187:1195	Enzyme extraction produced fucoidan with the most macrophage stimulation capacity (> 42 µmol).
30956301	9	36	theme	macrophage	1154:1163	arg1	capacity					1177:1184	the most macrophage stimulation capacity	1145:1184	the most macrophage stimulation capacity (> 42 µmol)	1145:1196	Enzyme extraction produced fucoidan with the most macrophage stimulation capacity (> 42 µmol).
30956301	10	37	theme	sulfate	1260:1266	arg1	groups					1268:1273	more sulfate groups	1255:1273	more sulfate groups	1255:1273	These results suggested that enzyme treatment preserved more sulfate groups in fucoidan structure influencing its anticancer and immunostimulatory activities.
30956301	9	38	theme	most	1149:1152	arg1	> 42 µmol					1187:1195	> 42 µmol	1187:1195	> 42 µmol	1187:1195	Enzyme extraction produced fucoidan with the most macrophage stimulation capacity (> 42 µmol).
30956301	9	38	theme	most	1149:1152	arg1	capacity					1177:1184	the most macrophage stimulation capacity	1145:1184	the most macrophage stimulation capacity (> 42 µmol)	1145:1196	Enzyme extraction produced fucoidan with the most macrophage stimulation capacity (> 42 µmol).
30956301	2	39	theme	anticancer	368:377	arg1	compositions					327:338	their chemical compositions	312:338	their chemical compositions	312:338	The extracted fucoidans were assessed for their chemical compositions, molecular characteristics, anticancer and immunomodulatory activities.
30956301	2	39	theme	anticancer	368:377	arg1	activities					400:409	anticancer and immunomodulatory activities	368:409	anticancer and immunomodulatory activities	368:409	The extracted fucoidans were assessed for their chemical compositions, molecular characteristics, anticancer and immunomodulatory activities.
30956301	10	40	theme	more	1255:1258	arg1	groups					1268:1273	more sulfate groups	1255:1273	more sulfate groups	1255:1273	These results suggested that enzyme treatment preserved more sulfate groups in fucoidan structure influencing its anticancer and immunostimulatory activities.
30956301	4	41	theme	proteins	633:640	arg1	levels					593:598	different levels	583:598	different levels of carbohydrates (52.78-58.65%), proteins (6.98-8.91%), sulfates (21.78-29.6%) and uronic acids (0.42-1.08%)	583:707	fucoidans were composed of different levels of carbohydrates (52.78-58.65%), proteins (6.98-8.91%), sulfates (21.78-29.6%) and uronic acids (0.42-1.08%).
30956301	7	42	theme	cancer	978:983	arg1	cells					985:989	HeLa and Hep-G2 cancer cells	962:989	HeLa and Hep-G2 cancer cells	962:989	All the recovered fucoidans showed strong growth inhibition against HeLa and Hep-G2 cancer cells.
30956301	3	43	theme	minimum	534:540	arg1	value					542:546	the minimum value	530:546	the minimum value (3.6%)	530:553	Enzyme-ultrasonic isolated fucoidan showed the maximum extraction yield (7.87%) while that obtained by ultrasonic had the minimum value (3.6%).
30956301	3	43	theme	minimum	534:540	arg1	%					552:552	3.6%	549:552	3.6%	549:552	Enzyme-ultrasonic isolated fucoidan showed the maximum extraction yield (7.87%) while that obtained by ultrasonic had the minimum value (3.6%).
30956301	1	44	theme	enzyme-ultrasonic	243:259	arg1	methods					261:267	enzyme-ultrasonic methods	243:267	enzyme-ultrasonic methods	243:267	Sulfated polysaccharide (fucoidan) was isolated from Nizamuddinia zanardinii by enzyme (alcalase), ultrasonic and enzyme-ultrasonic methods.
30956301	5	45	theme	molecular	734:742	arg1	weight					744:749	The weight mean average molecular weight	710:749	The weight mean average molecular weight of fucoidans	710:762	The weight mean average molecular weight of fucoidans varied between 443.7 and 1020.85 kDa.
30956301	5	46	theme	weight	714:719	arg1	weight					744:749	The weight mean average molecular weight	710:749	The weight mean average molecular weight of fucoidans	710:762	The weight mean average molecular weight of fucoidans varied between 443.7 and 1020.85 kDa.
30956301	2	47	theme	extracted	274:282	arg1	fucoidans					284:292	The extracted fucoidans	270:292	The extracted fucoidans	270:292	The extracted fucoidans were assessed for their chemical compositions, molecular characteristics, anticancer and immunomodulatory activities.
30956301	9	48	theme	stimulation	1165:1175	arg1	> 42 µmol					1187:1195	> 42 µmol	1187:1195	> 42 µmol	1187:1195	Enzyme extraction produced fucoidan with the most macrophage stimulation capacity (> 42 µmol).
30956301	9	48	theme	stimulation	1165:1175	arg1	capacity					1177:1184	the most macrophage stimulation capacity	1145:1184	the most macrophage stimulation capacity (> 42 µmol)	1145:1196	Enzyme extraction produced fucoidan with the most macrophage stimulation capacity (> 42 µmol).
30956301	4	49	theme	uronic	683:688	arg1	acids					690:694	uronic acids	683:694	uronic acids (0.42-1.08%)	683:707	fucoidans were composed of different levels of carbohydrates (52.78-58.65%), proteins (6.98-8.91%), sulfates (21.78-29.6%) and uronic acids (0.42-1.08%).
30956301	4	49	theme	uronic	683:688	arg1	%					706:706	0.42-1.08%	697:706	0.42-1.08%	697:706	fucoidans were composed of different levels of carbohydrates (52.78-58.65%), proteins (6.98-8.91%), sulfates (21.78-29.6%) and uronic acids (0.42-1.08%).
30956301	0	50	theme	Nizamuddinia	17:28	arg1	polysaccharides					50:64	Nizamuddinia zanardinii sulfated polysaccharides	17:64	Nizamuddinia zanardinii sulfated polysaccharides	17:64	Bioactivities of Nizamuddinia zanardinii sulfated polysaccharides extracted by enzyme, ultrasound and enzyme-ultrasound methods.
30956301	0	51	theme	enzyme-ultrasound	102:118	arg1	methods					120:126	enzyme-ultrasound methods	102:126	enzyme-ultrasound methods	102:126	Bioactivities of Nizamuddinia zanardinii sulfated polysaccharides extracted by enzyme, ultrasound and enzyme-ultrasound methods.
31254739	1	0	theme	traditional	159:169	arg1	manihot					133:139	Flos Abelmoschus manihot	116:139	Flos Abelmoschus manihot	116:139	Flos Abelmoschus manihot is widely used as traditional drug in China.
31254739	1	0	theme	traditional	159:169	arg1	drug					171:174	traditional drug	159:174	traditional drug in China	159:183	Flos Abelmoschus manihot is widely used as traditional drug in China.
31254739	4	1	theme	fatty	524:528	arg1	SCFAs					537:541	SCFAs	537:541	SCFAs	537:541	AM modified gut microbiota composition, increased microbial diversity, and in particularly, elevated the abundance of short chain fatty acids (SCFAs)-producing gut microbiota in colitic mice.
31254739	4	1	theme	fatty	524:528	arg1	acids					530:534	short chain fatty acids	512:534	short chain fatty acids (SCFAs)-producing gut microbiota in colitic mice	512:583	AM modified gut microbiota composition, increased microbial diversity, and in particularly, elevated the abundance of short chain fatty acids (SCFAs)-producing gut microbiota in colitic mice.
31254739	4	2	theme	gut	406:408	arg1	composition					421:431	gut microbiota composition	406:431	gut microbiota composition	406:431	AM modified gut microbiota composition, increased microbial diversity, and in particularly, elevated the abundance of short chain fatty acids (SCFAs)-producing gut microbiota in colitic mice.
31254739	4	3	theme	colitic	572:578	arg1	mice					580:583	colitic mice	572:583	colitic mice	572:583	AM modified gut microbiota composition, increased microbial diversity, and in particularly, elevated the abundance of short chain fatty acids (SCFAs)-producing gut microbiota in colitic mice.
31254739	3	4	theme	DSS-induced	365:375	arg1	colitis					377:383	DSS-induced colitis	365:383	DSS-induced colitis in mice	365:391	Here we showed that AM significantly alleviated DSS-induced colitis in mice.
31254739	4	5	theme	chain	518:522	arg1	SCFAs					537:541	SCFAs	537:541	SCFAs	537:541	AM modified gut microbiota composition, increased microbial diversity, and in particularly, elevated the abundance of short chain fatty acids (SCFAs)-producing gut microbiota in colitic mice.
31254739	4	5	theme	chain	518:522	arg1	acids					530:534	short chain fatty acids	512:534	short chain fatty acids (SCFAs)-producing gut microbiota in colitic mice	512:583	AM modified gut microbiota composition, increased microbial diversity, and in particularly, elevated the abundance of short chain fatty acids (SCFAs)-producing gut microbiota in colitic mice.
31254739	1	6	from	drug	171:174	arg1	China					179:183	China	179:183	China	179:183	Flos Abelmoschus manihot is widely used as traditional drug in China.
31254739	2	7	theme	chronic	286:292	arg1	diseases					307:314	chronic inflammatory diseases	286:314	chronic inflammatory diseases	286:314	Abelmoschus manihot (AM) extracted from Flos Abelmoschus manihot that has been applied for treating chronic inflammatory diseases.
31254739	2	8	theme	inflammatory	294:305	arg1	diseases					307:314	chronic inflammatory diseases	286:314	chronic inflammatory diseases	286:314	Abelmoschus manihot (AM) extracted from Flos Abelmoschus manihot that has been applied for treating chronic inflammatory diseases.
31254739	0	9	theme	Th17/Treg	97:105	arg1	balance					107:113	Th17/Treg balance	97:113	Th17/Treg balance	97:113	Flos Abelmoschus manihot extract attenuates DSS-induced colitis by regulating gut microbiota and Th17/Treg balance.
31254739	5	10	theme	AM	668:669	arg1	treatment					671:679	AM treatment	668:679	AM treatment	668:679	Consequently, levels of SCFAs especially butyrate and acetate were increased upon AM treatment, which, primarily through peroxisome proliferator-activated receptor gamma (PPARγ) pathway, led to the enhanced Treg generation and the suppressed Th17 development.
31254739	6	11	theme	gut	969:971	arg1	microbiome					973:982	gut microbiome	969:982	gut microbiome	969:982	Together, we herein provide the first evidences to support that AM, a natural plant-derived complex, can potentially reset gut microbiome and metabolism, resume immune and tissue homeostasis, and hence prevent colitis, which may provide a new perspective on IBD pathogenesis and suggest a novel microbiota-targeting therapy for inflammatory gut diseases.
31254739	6	12	theme	IBD	1104:1106	arg1	pathogenesis					1108:1119	IBD pathogenesis	1104:1119	IBD pathogenesis	1104:1119	Together, we herein provide the first evidences to support that AM, a natural plant-derived complex, can potentially reset gut microbiome and metabolism, resume immune and tissue homeostasis, and hence prevent colitis, which may provide a new perspective on IBD pathogenesis and suggest a novel microbiota-targeting therapy for inflammatory gut diseases.
31254739	4	13	theme	microbiota	410:419	arg1	composition					421:431	gut microbiota composition	406:431	gut microbiota composition	406:431	AM modified gut microbiota composition, increased microbial diversity, and in particularly, elevated the abundance of short chain fatty acids (SCFAs)-producing gut microbiota in colitic mice.
31254739	6	14	theme	microbiota-targeting	1141:1160	arg1	therapy					1162:1168	a novel microbiota-targeting therapy	1133:1168	a novel microbiota-targeting therapy for inflammatory gut diseases	1133:1198	Together, we herein provide the first evidences to support that AM, a natural plant-derived complex, can potentially reset gut microbiome and metabolism, resume immune and tissue homeostasis, and hence prevent colitis, which may provide a new perspective on IBD pathogenesis and suggest a novel microbiota-targeting therapy for inflammatory gut diseases.
31254739	6	15	theme	first	878:882	arg1	evidences					884:892	the first evidences	874:892	the first evidences to support that AM, a natural plant-derived complex, can potentially reset gut microbiome and metabolism, resume immune and tissue homeostasis, and hence prevent colitis	874:1062	Together, we herein provide the first evidences to support that AM, a natural plant-derived complex, can potentially reset gut microbiome and metabolism, resume immune and tissue homeostasis, and hence prevent colitis, which may provide a new perspective on IBD pathogenesis and suggest a novel microbiota-targeting therapy for inflammatory gut diseases.
31254739	1	16	used	used	151:154	arg2	drug					171:174	traditional drug	159:174	traditional drug in China	159:183	Flos Abelmoschus manihot is widely used as traditional drug in China.
31254739	1	16	used	used	151:154	arg2	manihot					133:139	Flos Abelmoschus manihot	116:139	Flos Abelmoschus manihot	116:139	Flos Abelmoschus manihot is widely used as traditional drug in China.
31254739	6	17	dep	provide	866:872	arg1	suggest					1125:1131	suggest	1125:1131	suggest a novel microbiota-targeting therapy for inflammatory gut diseases	1125:1198	Together, we herein provide the first evidences to support that AM, a natural plant-derived complex, can potentially reset gut microbiome and metabolism, resume immune and tissue homeostasis, and hence prevent colitis, which may provide a new perspective on IBD pathogenesis and suggest a novel microbiota-targeting therapy for inflammatory gut diseases.
31254739	6	17	dep	provide	866:872	arg1	provide					1075:1081	provide	1075:1081	provide a new perspective on IBD pathogenesis	1075:1119	Together, we herein provide the first evidences to support that AM, a natural plant-derived complex, can potentially reset gut microbiome and metabolism, resume immune and tissue homeostasis, and hence prevent colitis, which may provide a new perspective on IBD pathogenesis and suggest a novel microbiota-targeting therapy for inflammatory gut diseases.
31254739	0	18	theme	Flos	0:3	arg1	extract					25:31	Flos Abelmoschus manihot extract	0:31	Flos Abelmoschus manihot extract	0:31	Flos Abelmoschus manihot extract attenuates DSS-induced colitis by regulating gut microbiota and Th17/Treg balance.
31254739	4	19	theme	gut	554:556	arg1	microbiota					558:567	gut microbiota	554:567	gut microbiota in colitic mice	554:583	AM modified gut microbiota composition, increased microbial diversity, and in particularly, elevated the abundance of short chain fatty acids (SCFAs)-producing gut microbiota in colitic mice.
31254739	5	20	theme	receptor	741:748	arg1	PPARγ					757:761	PPARγ	757:761	PPARγ	757:761	Consequently, levels of SCFAs especially butyrate and acetate were increased upon AM treatment, which, primarily through peroxisome proliferator-activated receptor gamma (PPARγ) pathway, led to the enhanced Treg generation and the suppressed Th17 development.
31254739	5	20	theme	receptor	741:748	arg1	gamma					750:754	peroxisome proliferator-activated receptor gamma	707:754	peroxisome proliferator-activated receptor gamma (PPARγ) pathway	707:770	Consequently, levels of SCFAs especially butyrate and acetate were increased upon AM treatment, which, primarily through peroxisome proliferator-activated receptor gamma (PPARγ) pathway, led to the enhanced Treg generation and the suppressed Th17 development.
31254739	4	21	from	microbiota	558:567	arg1	mice					580:583	colitic mice	572:583	colitic mice	572:583	AM modified gut microbiota composition, increased microbial diversity, and in particularly, elevated the abundance of short chain fatty acids (SCFAs)-producing gut microbiota in colitic mice.
31254739	5	22	theme	SCFAs	610:614	arg1	levels					600:605	levels	600:605	levels of SCFAs especially butyrate and acetate	600:646	Consequently, levels of SCFAs especially butyrate and acetate were increased upon AM treatment, which, primarily through peroxisome proliferator-activated receptor gamma (PPARγ) pathway, led to the enhanced Treg generation and the suppressed Th17 development.
31254739	5	23	theme	Th17	828:831	arg1	development					833:843	the suppressed Th17 development	813:843	the suppressed Th17 development	813:843	Consequently, levels of SCFAs especially butyrate and acetate were increased upon AM treatment, which, primarily through peroxisome proliferator-activated receptor gamma (PPARγ) pathway, led to the enhanced Treg generation and the suppressed Th17 development.
31254739	0	24	theme	manihot	17:23	arg1	extract					25:31	Flos Abelmoschus manihot extract	0:31	Flos Abelmoschus manihot extract	0:31	Flos Abelmoschus manihot extract attenuates DSS-induced colitis by regulating gut microbiota and Th17/Treg balance.
31254739	2	25	theme	Abelmoschus	186:196	arg1	AM					207:208	AM	207:208	AM	207:208	Abelmoschus manihot (AM) extracted from Flos Abelmoschus manihot that has been applied for treating chronic inflammatory diseases.
31254739	2	25	theme	Abelmoschus	186:196	arg1	manihot					198:204	Abelmoschus manihot	186:204	Abelmoschus manihot (AM)	186:209	Abelmoschus manihot (AM) extracted from Flos Abelmoschus manihot that has been applied for treating chronic inflammatory diseases.
31254739	1	26	theme	Flos	116:119	arg1	manihot					133:139	Flos Abelmoschus manihot	116:139	Flos Abelmoschus manihot	116:139	Flos Abelmoschus manihot is widely used as traditional drug in China.
31254739	1	26	theme	Flos	116:119	arg1	drug					171:174	traditional drug	159:174	traditional drug in China	159:183	Flos Abelmoschus manihot is widely used as traditional drug in China.
31254739	0	27	theme	DSS-induced	44:54	arg1	colitis					56:62	DSS-induced colitis	44:62	DSS-induced colitis	44:62	Flos Abelmoschus manihot extract attenuates DSS-induced colitis by regulating gut microbiota and Th17/Treg balance.
31254739	5	28	theme	peroxisome	707:716	arg1	PPARγ					757:761	PPARγ	757:761	PPARγ	757:761	Consequently, levels of SCFAs especially butyrate and acetate were increased upon AM treatment, which, primarily through peroxisome proliferator-activated receptor gamma (PPARγ) pathway, led to the enhanced Treg generation and the suppressed Th17 development.
31254739	5	28	theme	peroxisome	707:716	arg1	gamma					750:754	peroxisome proliferator-activated receptor gamma	707:754	peroxisome proliferator-activated receptor gamma (PPARγ) pathway	707:770	Consequently, levels of SCFAs especially butyrate and acetate were increased upon AM treatment, which, primarily through peroxisome proliferator-activated receptor gamma (PPARγ) pathway, led to the enhanced Treg generation and the suppressed Th17 development.
31254739	6	29	theme	inflammatory	1174:1185	arg1	diseases					1191:1198	inflammatory gut diseases	1174:1198	inflammatory gut diseases	1174:1198	Together, we herein provide the first evidences to support that AM, a natural plant-derived complex, can potentially reset gut microbiome and metabolism, resume immune and tissue homeostasis, and hence prevent colitis, which may provide a new perspective on IBD pathogenesis and suggest a novel microbiota-targeting therapy for inflammatory gut diseases.
31254739	5	30	theme	proliferator-activated	718:739	arg1	PPARγ					757:761	PPARγ	757:761	PPARγ	757:761	Consequently, levels of SCFAs especially butyrate and acetate were increased upon AM treatment, which, primarily through peroxisome proliferator-activated receptor gamma (PPARγ) pathway, led to the enhanced Treg generation and the suppressed Th17 development.
31254739	5	30	theme	proliferator-activated	718:739	arg1	gamma					750:754	peroxisome proliferator-activated receptor gamma	707:754	peroxisome proliferator-activated receptor gamma (PPARγ) pathway	707:770	Consequently, levels of SCFAs especially butyrate and acetate were increased upon AM treatment, which, primarily through peroxisome proliferator-activated receptor gamma (PPARγ) pathway, led to the enhanced Treg generation and the suppressed Th17 development.
31254739	2	31	theme	Flos	226:229	arg1	manihot					243:249	Flos Abelmoschus manihot	226:249	Flos Abelmoschus manihot that has been applied for treating chronic inflammatory diseases	226:314	Abelmoschus manihot (AM) extracted from Flos Abelmoschus manihot that has been applied for treating chronic inflammatory diseases.
31254739	6	32	from	perspective	1089:1099	arg1	pathogenesis					1108:1119	IBD pathogenesis	1104:1119	IBD pathogenesis	1104:1119	Together, we herein provide the first evidences to support that AM, a natural plant-derived complex, can potentially reset gut microbiome and metabolism, resume immune and tissue homeostasis, and hence prevent colitis, which may provide a new perspective on IBD pathogenesis and suggest a novel microbiota-targeting therapy for inflammatory gut diseases.
31254739	2	33	dep	Flos	226:229	arg1	Abelmoschus					231:241	Abelmoschus	231:241	Abelmoschus	231:241	Abelmoschus manihot (AM) extracted from Flos Abelmoschus manihot that has been applied for treating chronic inflammatory diseases.
31254739	5	34	theme	enhanced	784:791	arg1	generation					798:807	the enhanced Treg generation	780:807	the enhanced Treg generation	780:807	Consequently, levels of SCFAs especially butyrate and acetate were increased upon AM treatment, which, primarily through peroxisome proliferator-activated receptor gamma (PPARγ) pathway, led to the enhanced Treg generation and the suppressed Th17 development.
31254739	4	35	theme	acids	530:534	arg1	abundance					499:507	the abundance	495:507	the abundance of short chain fatty acids (SCFAs)-producing gut microbiota in colitic mice	495:583	AM modified gut microbiota composition, increased microbial diversity, and in particularly, elevated the abundance of short chain fatty acids (SCFAs)-producing gut microbiota in colitic mice.
31254739	6	36	theme	tissue	1018:1023	arg1	homeostasis					1025:1035	immune and tissue homeostasis	1007:1035	homeostasis	1025:1035	Together, we herein provide the first evidences to support that AM, a natural plant-derived complex, can potentially reset gut microbiome and metabolism, resume immune and tissue homeostasis, and hence prevent colitis, which may provide a new perspective on IBD pathogenesis and suggest a novel microbiota-targeting therapy for inflammatory gut diseases.
31254739	3	37	from	colitis	377:383	arg1	mice					388:391	mice	388:391	mice	388:391	Here we showed that AM significantly alleviated DSS-induced colitis in mice.
31254739	1	38	theme	Abelmoschus	121:131	arg1	manihot					133:139	Flos Abelmoschus manihot	116:139	Flos Abelmoschus manihot	116:139	Flos Abelmoschus manihot is widely used as traditional drug in China.
31254739	1	38	theme	Abelmoschus	121:131	arg1	drug					171:174	traditional drug	159:174	traditional drug in China	159:183	Flos Abelmoschus manihot is widely used as traditional drug in China.
31254739	5	39	theme	Treg	793:796	arg1	generation					798:807	the enhanced Treg generation	780:807	the enhanced Treg generation	780:807	Consequently, levels of SCFAs especially butyrate and acetate were increased upon AM treatment, which, primarily through peroxisome proliferator-activated receptor gamma (PPARγ) pathway, led to the enhanced Treg generation and the suppressed Th17 development.
31254739	6	40	theme	natural	916:922	arg1	complex					938:944	a natural plant-derived complex	914:944	a natural plant-derived complex	914:944	Together, we herein provide the first evidences to support that AM, a natural plant-derived complex, can potentially reset gut microbiome and metabolism, resume immune and tissue homeostasis, and hence prevent colitis, which may provide a new perspective on IBD pathogenesis and suggest a novel microbiota-targeting therapy for inflammatory gut diseases.
31254739	6	40	theme	natural	916:922	arg1	AM					910:911	AM	910:911	AM	910:911	Together, we herein provide the first evidences to support that AM, a natural plant-derived complex, can potentially reset gut microbiome and metabolism, resume immune and tissue homeostasis, and hence prevent colitis, which may provide a new perspective on IBD pathogenesis and suggest a novel microbiota-targeting therapy for inflammatory gut diseases.
31254739	6	41	link	plant-derived	924:936	arg1	complex					938:944	a natural plant-derived complex	914:944	a natural plant-derived complex	914:944	Together, we herein provide the first evidences to support that AM, a natural plant-derived complex, can potentially reset gut microbiome and metabolism, resume immune and tissue homeostasis, and hence prevent colitis, which may provide a new perspective on IBD pathogenesis and suggest a novel microbiota-targeting therapy for inflammatory gut diseases.
31254739	6	41	link	plant-derived	924:936	arg1	AM					910:911	AM	910:911	AM	910:911	Together, we herein provide the first evidences to support that AM, a natural plant-derived complex, can potentially reset gut microbiome and metabolism, resume immune and tissue homeostasis, and hence prevent colitis, which may provide a new perspective on IBD pathogenesis and suggest a novel microbiota-targeting therapy for inflammatory gut diseases.
31254739	5	42	dep	SCFAs	610:614	arg1	acetate					640:646	acetate	640:646	acetate	640:646	Consequently, levels of SCFAs especially butyrate and acetate were increased upon AM treatment, which, primarily through peroxisome proliferator-activated receptor gamma (PPARγ) pathway, led to the enhanced Treg generation and the suppressed Th17 development.
31254739	5	42	dep	SCFAs	610:614	arg1	butyrate					627:634	butyrate	627:634	butyrate	627:634	Consequently, levels of SCFAs especially butyrate and acetate were increased upon AM treatment, which, primarily through peroxisome proliferator-activated receptor gamma (PPARγ) pathway, led to the enhanced Treg generation and the suppressed Th17 development.
31254739	6	43	theme	novel	1135:1139	arg1	therapy					1162:1168	a novel microbiota-targeting therapy	1133:1168	a novel microbiota-targeting therapy for inflammatory gut diseases	1133:1198	Together, we herein provide the first evidences to support that AM, a natural plant-derived complex, can potentially reset gut microbiome and metabolism, resume immune and tissue homeostasis, and hence prevent colitis, which may provide a new perspective on IBD pathogenesis and suggest a novel microbiota-targeting therapy for inflammatory gut diseases.
31254739	5	44	theme	suppressed	817:826	arg1	development					833:843	the suppressed Th17 development	813:843	the suppressed Th17 development	813:843	Consequently, levels of SCFAs especially butyrate and acetate were increased upon AM treatment, which, primarily through peroxisome proliferator-activated receptor gamma (PPARγ) pathway, led to the enhanced Treg generation and the suppressed Th17 development.
31254739	6	45	theme	new	1085:1087	arg1	perspective					1089:1099	a new perspective	1083:1099	a new perspective on IBD pathogenesis	1083:1119	Together, we herein provide the first evidences to support that AM, a natural plant-derived complex, can potentially reset gut microbiome and metabolism, resume immune and tissue homeostasis, and hence prevent colitis, which may provide a new perspective on IBD pathogenesis and suggest a novel microbiota-targeting therapy for inflammatory gut diseases.
31254739	6	46	theme	immune	1007:1012	arg1	homeostasis					1025:1035	immune and tissue homeostasis	1007:1035	homeostasis	1025:1035	Together, we herein provide the first evidences to support that AM, a natural plant-derived complex, can potentially reset gut microbiome and metabolism, resume immune and tissue homeostasis, and hence prevent colitis, which may provide a new perspective on IBD pathogenesis and suggest a novel microbiota-targeting therapy for inflammatory gut diseases.
31254739	0	47	theme	gut	78:80	arg1	microbiota					82:91	gut microbiota	78:91	gut microbiota	78:91	Flos Abelmoschus manihot extract attenuates DSS-induced colitis by regulating gut microbiota and Th17/Treg balance.
31254739	4	48	theme	microbial	444:452	arg1	diversity					454:462	microbial diversity	444:462	microbial diversity	444:462	AM modified gut microbiota composition, increased microbial diversity, and in particularly, elevated the abundance of short chain fatty acids (SCFAs)-producing gut microbiota in colitic mice.
31254739	6	49	theme	gut	1187:1189	arg1	diseases					1191:1198	inflammatory gut diseases	1174:1198	inflammatory gut diseases	1174:1198	Together, we herein provide the first evidences to support that AM, a natural plant-derived complex, can potentially reset gut microbiome and metabolism, resume immune and tissue homeostasis, and hence prevent colitis, which may provide a new perspective on IBD pathogenesis and suggest a novel microbiota-targeting therapy for inflammatory gut diseases.
31254739	4	50	theme	short	512:516	arg1	SCFAs					537:541	SCFAs	537:541	SCFAs	537:541	AM modified gut microbiota composition, increased microbial diversity, and in particularly, elevated the abundance of short chain fatty acids (SCFAs)-producing gut microbiota in colitic mice.
31254739	4	50	theme	short	512:516	arg1	acids					530:534	short chain fatty acids	512:534	short chain fatty acids (SCFAs)-producing gut microbiota in colitic mice	512:583	AM modified gut microbiota composition, increased microbial diversity, and in particularly, elevated the abundance of short chain fatty acids (SCFAs)-producing gut microbiota in colitic mice.
31254739	0	51	dep	Flos	0:3	arg1	Abelmoschus					5:15	Abelmoschus	5:15	Abelmoschus	5:15	Flos Abelmoschus manihot extract attenuates DSS-induced colitis by regulating gut microbiota and Th17/Treg balance.
31254739	6	52	theme	plant-derived	924:936	arg1	complex					938:944	a natural plant-derived complex	914:944	a natural plant-derived complex	914:944	Together, we herein provide the first evidences to support that AM, a natural plant-derived complex, can potentially reset gut microbiome and metabolism, resume immune and tissue homeostasis, and hence prevent colitis, which may provide a new perspective on IBD pathogenesis and suggest a novel microbiota-targeting therapy for inflammatory gut diseases.
31254739	6	52	theme	plant-derived	924:936	arg1	AM					910:911	AM	910:911	AM	910:911	Together, we herein provide the first evidences to support that AM, a natural plant-derived complex, can potentially reset gut microbiome and metabolism, resume immune and tissue homeostasis, and hence prevent colitis, which may provide a new perspective on IBD pathogenesis and suggest a novel microbiota-targeting therapy for inflammatory gut diseases.
31254739	5	53	theme	gamma	750:754	arg1	pathway					764:770	peroxisome proliferator-activated receptor gamma (PPARγ) pathway	707:770	peroxisome proliferator-activated receptor gamma (PPARγ) pathway	707:770	Consequently, levels of SCFAs especially butyrate and acetate were increased upon AM treatment, which, primarily through peroxisome proliferator-activated receptor gamma (PPARγ) pathway, led to the enhanced Treg generation and the suppressed Th17 development.
31212928	2	0	theme	total	550:554	arg1	phenol					556:561	total phenol and flavonoid contents	550:584	phenol	556:561	The results show that total phenol and flavonoid contents were both significantly higher in steamed versus raw GSEs, and the same trend was found for 2,2'-diphenyl-1-picrylhydrazyl (DPPH•) and 2,2'-azobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS•+) scavenging capacities.
31212928	3	1	from	GSEs	967:970	arg1	abundant					951:958	abundant	951:958	abundant	951:958	Among the 18 ginsenosides quantified using high-performance liquid chromatography (HPLC) with the aid of pure standards, polar ginsenosides were abundant in raw GSEs, whereas less-polar or rare ginsenosides appeared after steaming at 120 °C and increased with steaming time.
31212928	2	2	theme	scavenging	783:792	arg1	capacities					794:803	scavenging capacities	783:803	scavenging capacities	783:803	The results show that total phenol and flavonoid contents were both significantly higher in steamed versus raw GSEs, and the same trend was found for 2,2'-diphenyl-1-picrylhydrazyl (DPPH•) and 2,2'-azobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS•+) scavenging capacities.
31212928	4	3	theme	tumor	1270:1274	arg1	TNF					1293:1295	TNF	1293:1295	TNF	1293:1295	Furthermore, steamed GSEs exhibited a greater ability to inhibit the production of inflammatory mediators and pro-inflammatory cytokines, such as nitric oxide (NO), interleukin (IL)-6, and tumor necrosis factor (TNF)-α in LPS-induced RAW264.7 cells at the same concentration.
31212928	4	3	theme	tumor	1270:1274	arg1	factor					1285:1290	tumor necrosis factor	1270:1290	tumor necrosis factor (TNF)-α	1270:1298	Furthermore, steamed GSEs exhibited a greater ability to inhibit the production of inflammatory mediators and pro-inflammatory cytokines, such as nitric oxide (NO), interleukin (IL)-6, and tumor necrosis factor (TNF)-α in LPS-induced RAW264.7 cells at the same concentration.
31212928	1	4	theme	compounds	259:267	arg1	contents					238:245	contents	238:245	contents	238:245	We conducted this study for the first time to evaluate changes in the composition and contents of phenolic compounds and ginsenosides in ginseng shoot extracts (GSEs) prepared with different steaming times (2, 4, and 6 h) at 120 °C, as well as their antioxidant and anti-inflammatory activities in lipopolysaccharide (LPS)-induced RAW264.7 mouse macrophages (RAW264.7 cells).
31212928	1	4	theme	compounds	259:267	arg1	composition					222:232	the composition	218:232	the composition	218:232	We conducted this study for the first time to evaluate changes in the composition and contents of phenolic compounds and ginsenosides in ginseng shoot extracts (GSEs) prepared with different steaming times (2, 4, and 6 h) at 120 °C, as well as their antioxidant and anti-inflammatory activities in lipopolysaccharide (LPS)-induced RAW264.7 mouse macrophages (RAW264.7 cells).
31212928	1	5	theme	ginsenosides	273:284	arg1	contents					238:245	contents	238:245	contents	238:245	We conducted this study for the first time to evaluate changes in the composition and contents of phenolic compounds and ginsenosides in ginseng shoot extracts (GSEs) prepared with different steaming times (2, 4, and 6 h) at 120 °C, as well as their antioxidant and anti-inflammatory activities in lipopolysaccharide (LPS)-induced RAW264.7 mouse macrophages (RAW264.7 cells).
31212928	1	5	theme	ginsenosides	273:284	arg1	composition					222:232	the composition	218:232	the composition	218:232	We conducted this study for the first time to evaluate changes in the composition and contents of phenolic compounds and ginsenosides in ginseng shoot extracts (GSEs) prepared with different steaming times (2, 4, and 6 h) at 120 °C, as well as their antioxidant and anti-inflammatory activities in lipopolysaccharide (LPS)-induced RAW264.7 mouse macrophages (RAW264.7 cells).
31212928	5	6	from	enrichment	1524:1533	arg1	GSEs					1607:1610	steamed GSEs	1599:1610	steamed GSEs	1599:1610	Relative expression levels of inducible nitric oxide synthase (iNOS), IL-6, TNF-α, and cyclooxygenase-2 (COX-2) mRNAs were attenuated by the GSEs, probably due to the enrichment of less-polar ginsenosides and enhanced antioxidant activity in steamed GSEs.
31212928	3	7	theme	raw	963:965	arg1	GSEs					967:970	raw GSEs	963:970	raw GSEs	963:970	Among the 18 ginsenosides quantified using high-performance liquid chromatography (HPLC) with the aid of pure standards, polar ginsenosides were abundant in raw GSEs, whereas less-polar or rare ginsenosides appeared after steaming at 120 °C and increased with steaming time.
31212928	6	8	theme	inhibitory	1808:1817	arg1	effects					1819:1825	the inhibitory effects	1804:1825	the inhibitory effects of total phenols and total flavonoids	1804:1863	These findings, combined with correlation analysis, showed that less-polar ginsenosides were major contributors to the inhibition of the overproduction of various inflammatory factors, while the inhibitory effects of total phenols and total flavonoids, and their antioxidant abilities, are also important.
31212928	6	8	theme	inhibitory	1808:1817	arg1	important					1908:1916	important	1908:1916	important	1908:1916	These findings, combined with correlation analysis, showed that less-polar ginsenosides were major contributors to the inhibition of the overproduction of various inflammatory factors, while the inhibitory effects of total phenols and total flavonoids, and their antioxidant abilities, are also important.
31212928	1	9	theme	first	184:188	arg1	time					190:193	the first time	180:193	the first time	180:193	We conducted this study for the first time to evaluate changes in the composition and contents of phenolic compounds and ginsenosides in ginseng shoot extracts (GSEs) prepared with different steaming times (2, 4, and 6 h) at 120 °C, as well as their antioxidant and anti-inflammatory activities in lipopolysaccharide (LPS)-induced RAW264.7 mouse macrophages (RAW264.7 cells).
31212928	5	10	dep	synthase	1410:1417	arg1	mRNAs					1469:1473	mRNAs	1469:1473	mRNAs	1469:1473	Relative expression levels of inducible nitric oxide synthase (iNOS), IL-6, TNF-α, and cyclooxygenase-2 (COX-2) mRNAs were attenuated by the GSEs, probably due to the enrichment of less-polar ginsenosides and enhanced antioxidant activity in steamed GSEs.
31212928	3	11	theme	rare	995:998	arg1	ginsenosides					1000:1011	less-polar or rare ginsenosides	981:1011	less-polar or rare ginsenosides	981:1011	Among the 18 ginsenosides quantified using high-performance liquid chromatography (HPLC) with the aid of pure standards, polar ginsenosides were abundant in raw GSEs, whereas less-polar or rare ginsenosides appeared after steaming at 120 °C and increased with steaming time.
31212928	4	12	theme	mediators	1177:1185	arg1	production					1150:1159	the production	1146:1159	the production of inflammatory mediators and pro-inflammatory cytokines, such as nitric oxide (NO), interleukin (IL)-6, and tumor necrosis factor (TNF)-α in LPS-induced RAW264.7 cells	1146:1328	Furthermore, steamed GSEs exhibited a greater ability to inhibit the production of inflammatory mediators and pro-inflammatory cytokines, such as nitric oxide (NO), interleukin (IL)-6, and tumor necrosis factor (TNF)-α in LPS-induced RAW264.7 cells at the same concentration.
31212928	3	13	theme	less-polar	981:990	arg1	ginsenosides					1000:1011	less-polar or rare ginsenosides	981:1011	less-polar or rare ginsenosides	981:1011	Among the 18 ginsenosides quantified using high-performance liquid chromatography (HPLC) with the aid of pure standards, polar ginsenosides were abundant in raw GSEs, whereas less-polar or rare ginsenosides appeared after steaming at 120 °C and increased with steaming time.
31212928	6	14	theme	factors	1789:1795	arg1	overproduction					1750:1763	the overproduction	1746:1763	the overproduction of various inflammatory factors	1746:1795	These findings, combined with correlation analysis, showed that less-polar ginsenosides were major contributors to the inhibition of the overproduction of various inflammatory factors, while the inhibitory effects of total phenols and total flavonoids, and their antioxidant abilities, are also important.
31212928	4	15	theme	LPS-induced	1303:1313	arg1	cells					1324:1328	LPS-induced RAW264.7 cells	1303:1328	LPS-induced RAW264.7 cells	1303:1328	Furthermore, steamed GSEs exhibited a greater ability to inhibit the production of inflammatory mediators and pro-inflammatory cytokines, such as nitric oxide (NO), interleukin (IL)-6, and tumor necrosis factor (TNF)-α in LPS-induced RAW264.7 cells at the same concentration.
31212928	0	16	theme	Mouse	133:137	arg1	Macrophages					139:149	LPS-Induced RAW264.7 Mouse Macrophages	112:149	LPS-Induced RAW264.7 Mouse Macrophages	112:149	Phenolic Compounds and Ginsenosides in Ginseng Shoots and Their Antioxidant and Anti-Inflammatory Capacities in LPS-Induced RAW264.7 Mouse Macrophages.
31212928	6	17	theme	various	1768:1774	arg1	factors					1789:1795	various inflammatory factors	1768:1795	various inflammatory factors	1768:1795	These findings, combined with correlation analysis, showed that less-polar ginsenosides were major contributors to the inhibition of the overproduction of various inflammatory factors, while the inhibitory effects of total phenols and total flavonoids, and their antioxidant abilities, are also important.
31212928	4	18	theme	factor	1285:1290	arg1	-α					1297:1298	tumor necrosis factor (TNF)-α	1270:1298	tumor necrosis factor (TNF)-α	1270:1298	Furthermore, steamed GSEs exhibited a greater ability to inhibit the production of inflammatory mediators and pro-inflammatory cytokines, such as nitric oxide (NO), interleukin (IL)-6, and tumor necrosis factor (TNF)-α in LPS-induced RAW264.7 cells at the same concentration.
31212928	6	19	theme	total	1848:1852	arg1	flavonoids					1854:1863	total flavonoids	1848:1863	total flavonoids	1848:1863	These findings, combined with correlation analysis, showed that less-polar ginsenosides were major contributors to the inhibition of the overproduction of various inflammatory factors, while the inhibitory effects of total phenols and total flavonoids, and their antioxidant abilities, are also important.
31212928	0	20	from	Capacities	98:107	arg1	Shoots					47:52	Ginseng Shoots	39:52	Ginseng Shoots	39:52	Phenolic Compounds and Ginsenosides in Ginseng Shoots and Their Antioxidant and Anti-Inflammatory Capacities in LPS-Induced RAW264.7 Mouse Macrophages.
31212928	0	20	from	Capacities	98:107	arg1	Macrophages					139:149	LPS-Induced RAW264.7 Mouse Macrophages	112:149	LPS-Induced RAW264.7 Mouse Macrophages	112:149	Phenolic Compounds and Ginsenosides in Ginseng Shoots and Their Antioxidant and Anti-Inflammatory Capacities in LPS-Induced RAW264.7 Mouse Macrophages.
31212928	6	21	theme	phenols	1836:1842	arg1	effects					1819:1825	the inhibitory effects	1804:1825	the inhibitory effects of total phenols and total flavonoids	1804:1863	These findings, combined with correlation analysis, showed that less-polar ginsenosides were major contributors to the inhibition of the overproduction of various inflammatory factors, while the inhibitory effects of total phenols and total flavonoids, and their antioxidant abilities, are also important.
31212928	6	21	theme	phenols	1836:1842	arg1	abilities					1888:1896	their antioxidant abilities	1870:1896	their antioxidant abilities	1870:1896	These findings, combined with correlation analysis, showed that less-polar ginsenosides were major contributors to the inhibition of the overproduction of various inflammatory factors, while the inhibitory effects of total phenols and total flavonoids, and their antioxidant abilities, are also important.
31212928	6	21	theme	phenols	1836:1842	arg1	important					1908:1916	important	1908:1916	important	1908:1916	These findings, combined with correlation analysis, showed that less-polar ginsenosides were major contributors to the inhibition of the overproduction of various inflammatory factors, while the inhibitory effects of total phenols and total flavonoids, and their antioxidant abilities, are also important.
31212928	1	22	from	°C	381:382	arg1	antioxidant					402:412	their antioxidant	396:412	their antioxidant	396:412	We conducted this study for the first time to evaluate changes in the composition and contents of phenolic compounds and ginsenosides in ginseng shoot extracts (GSEs) prepared with different steaming times (2, 4, and 6 h) at 120 °C, as well as their antioxidant and anti-inflammatory activities in lipopolysaccharide (LPS)-induced RAW264.7 mouse macrophages (RAW264.7 cells).
31212928	1	22	from	°C	381:382	arg1	activities					436:445	anti-inflammatory activities	418:445	anti-inflammatory activities in lipopolysaccharide (LPS)-induced RAW264.7 mouse macrophages (RAW264.7 cells)	418:525	We conducted this study for the first time to evaluate changes in the composition and contents of phenolic compounds and ginsenosides in ginseng shoot extracts (GSEs) prepared with different steaming times (2, 4, and 6 h) at 120 °C, as well as their antioxidant and anti-inflammatory activities in lipopolysaccharide (LPS)-induced RAW264.7 mouse macrophages (RAW264.7 cells).
31212928	1	22	from	°C	381:382	arg1	times					352:356	different steaming times	333:356	different steaming times (2, 4, and 6 h) at 120 °C	333:382	We conducted this study for the first time to evaluate changes in the composition and contents of phenolic compounds and ginsenosides in ginseng shoot extracts (GSEs) prepared with different steaming times (2, 4, and 6 h) at 120 °C, as well as their antioxidant and anti-inflammatory activities in lipopolysaccharide (LPS)-induced RAW264.7 mouse macrophages (RAW264.7 cells).
31212928	1	22	from	°C	381:382	arg1	h					371:371	2, 4, and 6 h	359:371	2, 4, and 6 h	359:371	We conducted this study for the first time to evaluate changes in the composition and contents of phenolic compounds and ginsenosides in ginseng shoot extracts (GSEs) prepared with different steaming times (2, 4, and 6 h) at 120 °C, as well as their antioxidant and anti-inflammatory activities in lipopolysaccharide (LPS)-induced RAW264.7 mouse macrophages (RAW264.7 cells).
31212928	3	23	dep	the	900:902	arg1	aid					904:906	aid	904:906	aid	904:906	Among the 18 ginsenosides quantified using high-performance liquid chromatography (HPLC) with the aid of pure standards, polar ginsenosides were abundant in raw GSEs, whereas less-polar or rare ginsenosides appeared after steaming at 120 °C and increased with steaming time.
31212928	5	24	theme	nitric	1397:1402	arg1	iNOS					1420:1423	iNOS	1420:1423	iNOS	1420:1423	Relative expression levels of inducible nitric oxide synthase (iNOS), IL-6, TNF-α, and cyclooxygenase-2 (COX-2) mRNAs were attenuated by the GSEs, probably due to the enrichment of less-polar ginsenosides and enhanced antioxidant activity in steamed GSEs.
31212928	5	24	theme	nitric	1397:1402	arg1	synthase					1410:1417	inducible nitric oxide synthase	1387:1417	inducible nitric oxide synthase (iNOS)	1387:1424	Relative expression levels of inducible nitric oxide synthase (iNOS), IL-6, TNF-α, and cyclooxygenase-2 (COX-2) mRNAs were attenuated by the GSEs, probably due to the enrichment of less-polar ginsenosides and enhanced antioxidant activity in steamed GSEs.
31212928	2	25	theme	same	653:656	arg1	trend					658:662	the same trend	649:662	the same trend	649:662	The results show that total phenol and flavonoid contents were both significantly higher in steamed versus raw GSEs, and the same trend was found for 2,2'-diphenyl-1-picrylhydrazyl (DPPH•) and 2,2'-azobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS•+) scavenging capacities.
31212928	1	26	theme	steaming	343:350	arg1	times					352:356	different steaming times	333:356	different steaming times (2, 4, and 6 h) at 120 °C	333:382	We conducted this study for the first time to evaluate changes in the composition and contents of phenolic compounds and ginsenosides in ginseng shoot extracts (GSEs) prepared with different steaming times (2, 4, and 6 h) at 120 °C, as well as their antioxidant and anti-inflammatory activities in lipopolysaccharide (LPS)-induced RAW264.7 mouse macrophages (RAW264.7 cells).
31212928	1	26	theme	steaming	343:350	arg1	h					371:371	2, 4, and 6 h	359:371	2, 4, and 6 h	359:371	We conducted this study for the first time to evaluate changes in the composition and contents of phenolic compounds and ginsenosides in ginseng shoot extracts (GSEs) prepared with different steaming times (2, 4, and 6 h) at 120 °C, as well as their antioxidant and anti-inflammatory activities in lipopolysaccharide (LPS)-induced RAW264.7 mouse macrophages (RAW264.7 cells).
31212928	2	27	dep	higher	610:615	arg1	both					591:594	both	591:594	both	591:594	The results show that total phenol and flavonoid contents were both significantly higher in steamed versus raw GSEs, and the same trend was found for 2,2'-diphenyl-1-picrylhydrazyl (DPPH•) and 2,2'-azobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS•+) scavenging capacities.
31212928	5	28	theme	synthase	1410:1417	arg1	levels					1377:1382	Relative expression levels	1357:1382	Relative expression levels of inducible nitric oxide synthase (iNOS), IL-6, TNF-α, and cyclooxygenase-2 (COX-2) mRNAs	1357:1473	Relative expression levels of inducible nitric oxide synthase (iNOS), IL-6, TNF-α, and cyclooxygenase-2 (COX-2) mRNAs were attenuated by the GSEs, probably due to the enrichment of less-polar ginsenosides and enhanced antioxidant activity in steamed GSEs.
31212928	1	29	dep	lipopolysaccharide	450:467	arg1	macrophages					498:508	-induced RAW264.7 mouse macrophages	474:508	lipopolysaccharide (LPS)-induced RAW264.7 mouse macrophages (RAW264.7 cells)	450:525	We conducted this study for the first time to evaluate changes in the composition and contents of phenolic compounds and ginsenosides in ginseng shoot extracts (GSEs) prepared with different steaming times (2, 4, and 6 h) at 120 °C, as well as their antioxidant and anti-inflammatory activities in lipopolysaccharide (LPS)-induced RAW264.7 mouse macrophages (RAW264.7 cells).
31212928	1	29	dep	lipopolysaccharide	450:467	arg1	cells					520:524	RAW264.7 cells	511:524	RAW264.7 cells	511:524	We conducted this study for the first time to evaluate changes in the composition and contents of phenolic compounds and ginsenosides in ginseng shoot extracts (GSEs) prepared with different steaming times (2, 4, and 6 h) at 120 °C, as well as their antioxidant and anti-inflammatory activities in lipopolysaccharide (LPS)-induced RAW264.7 mouse macrophages (RAW264.7 cells).
31212928	5	30	theme	expression	1366:1375	arg1	levels					1377:1382	Relative expression levels	1357:1382	Relative expression levels of inducible nitric oxide synthase (iNOS), IL-6, TNF-α, and cyclooxygenase-2 (COX-2) mRNAs	1357:1473	Relative expression levels of inducible nitric oxide synthase (iNOS), IL-6, TNF-α, and cyclooxygenase-2 (COX-2) mRNAs were attenuated by the GSEs, probably due to the enrichment of less-polar ginsenosides and enhanced antioxidant activity in steamed GSEs.
31212928	4	31	theme	cytokines	1208:1216	arg1	production					1150:1159	the production	1146:1159	the production of inflammatory mediators and pro-inflammatory cytokines, such as nitric oxide (NO), interleukin (IL)-6, and tumor necrosis factor (TNF)-α in LPS-induced RAW264.7 cells	1146:1328	Furthermore, steamed GSEs exhibited a greater ability to inhibit the production of inflammatory mediators and pro-inflammatory cytokines, such as nitric oxide (NO), interleukin (IL)-6, and tumor necrosis factor (TNF)-α in LPS-induced RAW264.7 cells at the same concentration.
31212928	5	32	theme	enhanced	1566:1573	arg1	activity					1587:1594	enhanced antioxidant activity	1566:1594	enhanced antioxidant activity in steamed GSEs	1566:1610	Relative expression levels of inducible nitric oxide synthase (iNOS), IL-6, TNF-α, and cyclooxygenase-2 (COX-2) mRNAs were attenuated by the GSEs, probably due to the enrichment of less-polar ginsenosides and enhanced antioxidant activity in steamed GSEs.
31212928	0	33	theme	LPS-Induced	112:122	arg1	Macrophages					139:149	LPS-Induced RAW264.7 Mouse Macrophages	112:149	LPS-Induced RAW264.7 Mouse Macrophages	112:149	Phenolic Compounds and Ginsenosides in Ginseng Shoots and Their Antioxidant and Anti-Inflammatory Capacities in LPS-Induced RAW264.7 Mouse Macrophages.
31212928	3	34	theme	liquid	866:871	arg1	HPLC					889:892	HPLC	889:892	HPLC	889:892	Among the 18 ginsenosides quantified using high-performance liquid chromatography (HPLC) with the aid of pure standards, polar ginsenosides were abundant in raw GSEs, whereas less-polar or rare ginsenosides appeared after steaming at 120 °C and increased with steaming time.
31212928	3	34	theme	liquid	866:871	arg1	chromatography					873:886	high-performance liquid chromatography	849:886	high-performance liquid chromatography (HPLC)	849:893	Among the 18 ginsenosides quantified using high-performance liquid chromatography (HPLC) with the aid of pure standards, polar ginsenosides were abundant in raw GSEs, whereas less-polar or rare ginsenosides appeared after steaming at 120 °C and increased with steaming time.
31212928	1	35	theme	RAW264.7	511:518	arg1	macrophages					498:508	-induced RAW264.7 mouse macrophages	474:508	lipopolysaccharide (LPS)-induced RAW264.7 mouse macrophages (RAW264.7 cells)	450:525	We conducted this study for the first time to evaluate changes in the composition and contents of phenolic compounds and ginsenosides in ginseng shoot extracts (GSEs) prepared with different steaming times (2, 4, and 6 h) at 120 °C, as well as their antioxidant and anti-inflammatory activities in lipopolysaccharide (LPS)-induced RAW264.7 mouse macrophages (RAW264.7 cells).
31212928	1	35	theme	RAW264.7	511:518	arg1	cells					520:524	RAW264.7 cells	511:524	RAW264.7 cells	511:524	We conducted this study for the first time to evaluate changes in the composition and contents of phenolic compounds and ginsenosides in ginseng shoot extracts (GSEs) prepared with different steaming times (2, 4, and 6 h) at 120 °C, as well as their antioxidant and anti-inflammatory activities in lipopolysaccharide (LPS)-induced RAW264.7 mouse macrophages (RAW264.7 cells).
31212928	0	36	theme	Phenolic	0:7	arg1	Compounds					9:17	Phenolic Compounds	0:17	Phenolic Compounds	0:17	Phenolic Compounds and Ginsenosides in Ginseng Shoots and Their Antioxidant and Anti-Inflammatory Capacities in LPS-Induced RAW264.7 Mouse Macrophages.
31212928	3	37	from	abundant	951:958	arg1	GSEs					967:970	raw GSEs	963:970	raw GSEs	963:970	Among the 18 ginsenosides quantified using high-performance liquid chromatography (HPLC) with the aid of pure standards, polar ginsenosides were abundant in raw GSEs, whereas less-polar or rare ginsenosides appeared after steaming at 120 °C and increased with steaming time.
31212928	4	38	theme	inflammatory	1164:1175	arg1	IL					1259:1260	interleukin (IL)-6	1246:1263	interleukin (IL)-6	1246:1263	Furthermore, steamed GSEs exhibited a greater ability to inhibit the production of inflammatory mediators and pro-inflammatory cytokines, such as nitric oxide (NO), interleukin (IL)-6, and tumor necrosis factor (TNF)-α in LPS-induced RAW264.7 cells at the same concentration.
31212928	4	38	theme	inflammatory	1164:1175	arg1	-α					1297:1298	tumor necrosis factor (TNF)-α	1270:1298	tumor necrosis factor (TNF)-α	1270:1298	Furthermore, steamed GSEs exhibited a greater ability to inhibit the production of inflammatory mediators and pro-inflammatory cytokines, such as nitric oxide (NO), interleukin (IL)-6, and tumor necrosis factor (TNF)-α in LPS-induced RAW264.7 cells at the same concentration.
31212928	4	38	theme	inflammatory	1164:1175	arg1	mediators					1177:1185	inflammatory mediators	1164:1185	inflammatory mediators	1164:1185	Furthermore, steamed GSEs exhibited a greater ability to inhibit the production of inflammatory mediators and pro-inflammatory cytokines, such as nitric oxide (NO), interleukin (IL)-6, and tumor necrosis factor (TNF)-α in LPS-induced RAW264.7 cells at the same concentration.
31212928	4	38	theme	inflammatory	1164:1175	arg1	oxide					1234:1238	nitric oxide	1227:1238	nitric oxide (NO)	1227:1243	Furthermore, steamed GSEs exhibited a greater ability to inhibit the production of inflammatory mediators and pro-inflammatory cytokines, such as nitric oxide (NO), interleukin (IL)-6, and tumor necrosis factor (TNF)-α in LPS-induced RAW264.7 cells at the same concentration.
31212928	1	39	theme	shoot	297:301	arg1	GSEs					313:316	GSEs	313:316	GSEs	313:316	We conducted this study for the first time to evaluate changes in the composition and contents of phenolic compounds and ginsenosides in ginseng shoot extracts (GSEs) prepared with different steaming times (2, 4, and 6 h) at 120 °C, as well as their antioxidant and anti-inflammatory activities in lipopolysaccharide (LPS)-induced RAW264.7 mouse macrophages (RAW264.7 cells).
31212928	1	39	theme	shoot	297:301	arg1	extracts					303:310	ginseng shoot extracts	289:310	ginseng shoot extracts (GSEs) prepared with different steaming times (2, 4, and 6 h) at 120 °C, as well as their antioxidant and anti-inflammatory activities in lipopolysaccharide (LPS)-induced RAW264.7 mouse macrophages (RAW264.7 cells)	289:525	We conducted this study for the first time to evaluate changes in the composition and contents of phenolic compounds and ginsenosides in ginseng shoot extracts (GSEs) prepared with different steaming times (2, 4, and 6 h) at 120 °C, as well as their antioxidant and anti-inflammatory activities in lipopolysaccharide (LPS)-induced RAW264.7 mouse macrophages (RAW264.7 cells).
31212928	4	40	from	production	1150:1159	arg1	cells					1324:1328	LPS-induced RAW264.7 cells	1303:1328	LPS-induced RAW264.7 cells	1303:1328	Furthermore, steamed GSEs exhibited a greater ability to inhibit the production of inflammatory mediators and pro-inflammatory cytokines, such as nitric oxide (NO), interleukin (IL)-6, and tumor necrosis factor (TNF)-α in LPS-induced RAW264.7 cells at the same concentration.
31212928	5	41	theme	ginsenosides	1549:1560	arg1	activity					1587:1594	enhanced antioxidant activity	1566:1594	enhanced antioxidant activity in steamed GSEs	1566:1610	Relative expression levels of inducible nitric oxide synthase (iNOS), IL-6, TNF-α, and cyclooxygenase-2 (COX-2) mRNAs were attenuated by the GSEs, probably due to the enrichment of less-polar ginsenosides and enhanced antioxidant activity in steamed GSEs.
31212928	5	41	theme	ginsenosides	1549:1560	arg1	enrichment					1524:1533	the enrichment	1520:1533	the enrichment of less-polar ginsenosides	1520:1560	Relative expression levels of inducible nitric oxide synthase (iNOS), IL-6, TNF-α, and cyclooxygenase-2 (COX-2) mRNAs were attenuated by the GSEs, probably due to the enrichment of less-polar ginsenosides and enhanced antioxidant activity in steamed GSEs.
31212928	1	42	from	times	352:356	arg1	lipopolysaccharide					450:467	lipopolysaccharide	450:467	lipopolysaccharide (LPS)-induced RAW264.7 mouse macrophages (RAW264.7 cells)	450:525	We conducted this study for the first time to evaluate changes in the composition and contents of phenolic compounds and ginsenosides in ginseng shoot extracts (GSEs) prepared with different steaming times (2, 4, and 6 h) at 120 °C, as well as their antioxidant and anti-inflammatory activities in lipopolysaccharide (LPS)-induced RAW264.7 mouse macrophages (RAW264.7 cells).
31212928	1	42	from	times	352:356	arg1	LPS					470:472	LPS	470:472	LPS	470:472	We conducted this study for the first time to evaluate changes in the composition and contents of phenolic compounds and ginsenosides in ginseng shoot extracts (GSEs) prepared with different steaming times (2, 4, and 6 h) at 120 °C, as well as their antioxidant and anti-inflammatory activities in lipopolysaccharide (LPS)-induced RAW264.7 mouse macrophages (RAW264.7 cells).
31212928	6	43	theme	correlation	1643:1653	arg1	analysis					1655:1662	correlation analysis	1643:1662	correlation analysis	1643:1662	These findings, combined with correlation analysis, showed that less-polar ginsenosides were major contributors to the inhibition of the overproduction of various inflammatory factors, while the inhibitory effects of total phenols and total flavonoids, and their antioxidant abilities, are also important.
31212928	5	44	from	activity	1587:1594	arg1	GSEs					1607:1610	steamed GSEs	1599:1610	steamed GSEs	1599:1610	Relative expression levels of inducible nitric oxide synthase (iNOS), IL-6, TNF-α, and cyclooxygenase-2 (COX-2) mRNAs were attenuated by the GSEs, probably due to the enrichment of less-polar ginsenosides and enhanced antioxidant activity in steamed GSEs.
31212928	1	45	from	antioxidant	402:412	arg1	lipopolysaccharide					450:467	lipopolysaccharide	450:467	lipopolysaccharide (LPS)-induced RAW264.7 mouse macrophages (RAW264.7 cells)	450:525	We conducted this study for the first time to evaluate changes in the composition and contents of phenolic compounds and ginsenosides in ginseng shoot extracts (GSEs) prepared with different steaming times (2, 4, and 6 h) at 120 °C, as well as their antioxidant and anti-inflammatory activities in lipopolysaccharide (LPS)-induced RAW264.7 mouse macrophages (RAW264.7 cells).
31212928	1	45	from	antioxidant	402:412	arg1	LPS					470:472	LPS	470:472	LPS	470:472	We conducted this study for the first time to evaluate changes in the composition and contents of phenolic compounds and ginsenosides in ginseng shoot extracts (GSEs) prepared with different steaming times (2, 4, and 6 h) at 120 °C, as well as their antioxidant and anti-inflammatory activities in lipopolysaccharide (LPS)-induced RAW264.7 mouse macrophages (RAW264.7 cells).
31212928	4	46	theme	nitric	1227:1232	arg1	NO					1241:1242	NO	1241:1242	NO	1241:1242	Furthermore, steamed GSEs exhibited a greater ability to inhibit the production of inflammatory mediators and pro-inflammatory cytokines, such as nitric oxide (NO), interleukin (IL)-6, and tumor necrosis factor (TNF)-α in LPS-induced RAW264.7 cells at the same concentration.
31212928	4	46	theme	nitric	1227:1232	arg1	oxide					1234:1238	nitric oxide	1227:1238	nitric oxide (NO)	1227:1243	Furthermore, steamed GSEs exhibited a greater ability to inhibit the production of inflammatory mediators and pro-inflammatory cytokines, such as nitric oxide (NO), interleukin (IL)-6, and tumor necrosis factor (TNF)-α in LPS-induced RAW264.7 cells at the same concentration.
31212928	5	47	theme	steamed	1599:1605	arg1	GSEs					1607:1610	steamed GSEs	1599:1610	steamed GSEs	1599:1610	Relative expression levels of inducible nitric oxide synthase (iNOS), IL-6, TNF-α, and cyclooxygenase-2 (COX-2) mRNAs were attenuated by the GSEs, probably due to the enrichment of less-polar ginsenosides and enhanced antioxidant activity in steamed GSEs.
31212928	3	48	theme	polar	927:931	arg1	ginsenosides					933:944	polar ginsenosides	927:944	polar ginsenosides	927:944	Among the 18 ginsenosides quantified using high-performance liquid chromatography (HPLC) with the aid of pure standards, polar ginsenosides were abundant in raw GSEs, whereas less-polar or rare ginsenosides appeared after steaming at 120 °C and increased with steaming time.
31212928	1	49	theme	RAW264.7	483:490	arg1	macrophages					498:508	-induced RAW264.7 mouse macrophages	474:508	lipopolysaccharide (LPS)-induced RAW264.7 mouse macrophages (RAW264.7 cells)	450:525	We conducted this study for the first time to evaluate changes in the composition and contents of phenolic compounds and ginsenosides in ginseng shoot extracts (GSEs) prepared with different steaming times (2, 4, and 6 h) at 120 °C, as well as their antioxidant and anti-inflammatory activities in lipopolysaccharide (LPS)-induced RAW264.7 mouse macrophages (RAW264.7 cells).
31212928	1	49	theme	RAW264.7	483:490	arg1	cells					520:524	RAW264.7 cells	511:524	RAW264.7 cells	511:524	We conducted this study for the first time to evaluate changes in the composition and contents of phenolic compounds and ginsenosides in ginseng shoot extracts (GSEs) prepared with different steaming times (2, 4, and 6 h) at 120 °C, as well as their antioxidant and anti-inflammatory activities in lipopolysaccharide (LPS)-induced RAW264.7 mouse macrophages (RAW264.7 cells).
31212928	1	50	theme	phenolic	250:257	arg1	compounds					259:267	phenolic compounds	250:267	phenolic compounds	250:267	We conducted this study for the first time to evaluate changes in the composition and contents of phenolic compounds and ginsenosides in ginseng shoot extracts (GSEs) prepared with different steaming times (2, 4, and 6 h) at 120 °C, as well as their antioxidant and anti-inflammatory activities in lipopolysaccharide (LPS)-induced RAW264.7 mouse macrophages (RAW264.7 cells).
31212928	5	51	theme	cyclooxygenase-2	1444:1459	arg1	levels					1377:1382	Relative expression levels	1357:1382	Relative expression levels of inducible nitric oxide synthase (iNOS), IL-6, TNF-α, and cyclooxygenase-2 (COX-2) mRNAs	1357:1473	Relative expression levels of inducible nitric oxide synthase (iNOS), IL-6, TNF-α, and cyclooxygenase-2 (COX-2) mRNAs were attenuated by the GSEs, probably due to the enrichment of less-polar ginsenosides and enhanced antioxidant activity in steamed GSEs.
31212928	6	52	theme	major	1706:1710	arg1	contributors					1712:1723	major contributors	1706:1723	major contributors to the inhibition of the overproduction of various inflammatory factors	1706:1795	These findings, combined with correlation analysis, showed that less-polar ginsenosides were major contributors to the inhibition of the overproduction of various inflammatory factors, while the inhibitory effects of total phenols and total flavonoids, and their antioxidant abilities, are also important.
31212928	4	53	theme	interleukin	1246:1256	arg1	IL					1259:1260	interleukin (IL)-6	1246:1263	interleukin (IL)-6	1246:1263	Furthermore, steamed GSEs exhibited a greater ability to inhibit the production of inflammatory mediators and pro-inflammatory cytokines, such as nitric oxide (NO), interleukin (IL)-6, and tumor necrosis factor (TNF)-α in LPS-induced RAW264.7 cells at the same concentration.
31212928	5	54	theme	Relative	1357:1364	arg1	levels					1377:1382	Relative expression levels	1357:1382	Relative expression levels of inducible nitric oxide synthase (iNOS), IL-6, TNF-α, and cyclooxygenase-2 (COX-2) mRNAs	1357:1473	Relative expression levels of inducible nitric oxide synthase (iNOS), IL-6, TNF-α, and cyclooxygenase-2 (COX-2) mRNAs were attenuated by the GSEs, probably due to the enrichment of less-polar ginsenosides and enhanced antioxidant activity in steamed GSEs.
31212928	2	55	theme	flavonoid	567:575	arg1	contents					577:584	total phenol and flavonoid contents	550:584	contents	577:584	The results show that total phenol and flavonoid contents were both significantly higher in steamed versus raw GSEs, and the same trend was found for 2,2'-diphenyl-1-picrylhydrazyl (DPPH•) and 2,2'-azobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS•+) scavenging capacities.
31212928	4	56	theme	steamed	1094:1100	arg1	GSEs					1102:1105	steamed GSEs	1094:1105	steamed GSEs	1094:1105	Furthermore, steamed GSEs exhibited a greater ability to inhibit the production of inflammatory mediators and pro-inflammatory cytokines, such as nitric oxide (NO), interleukin (IL)-6, and tumor necrosis factor (TNF)-α in LPS-induced RAW264.7 cells at the same concentration.
31212928	1	57	theme	anti-inflammatory	418:434	arg1	activities					436:445	anti-inflammatory activities	418:445	anti-inflammatory activities in lipopolysaccharide (LPS)-induced RAW264.7 mouse macrophages (RAW264.7 cells)	418:525	We conducted this study for the first time to evaluate changes in the composition and contents of phenolic compounds and ginsenosides in ginseng shoot extracts (GSEs) prepared with different steaming times (2, 4, and 6 h) at 120 °C, as well as their antioxidant and anti-inflammatory activities in lipopolysaccharide (LPS)-induced RAW264.7 mouse macrophages (RAW264.7 cells).
31212928	6	58	theme	inflammatory	1776:1787	arg1	factors					1789:1795	various inflammatory factors	1768:1795	various inflammatory factors	1768:1795	These findings, combined with correlation analysis, showed that less-polar ginsenosides were major contributors to the inhibition of the overproduction of various inflammatory factors, while the inhibitory effects of total phenols and total flavonoids, and their antioxidant abilities, are also important.
31212928	4	59	theme	necrosis	1276:1283	arg1	TNF					1293:1295	TNF	1293:1295	TNF	1293:1295	Furthermore, steamed GSEs exhibited a greater ability to inhibit the production of inflammatory mediators and pro-inflammatory cytokines, such as nitric oxide (NO), interleukin (IL)-6, and tumor necrosis factor (TNF)-α in LPS-induced RAW264.7 cells at the same concentration.
31212928	4	59	theme	necrosis	1276:1283	arg1	factor					1285:1290	tumor necrosis factor	1270:1290	tumor necrosis factor (TNF)-α	1270:1298	Furthermore, steamed GSEs exhibited a greater ability to inhibit the production of inflammatory mediators and pro-inflammatory cytokines, such as nitric oxide (NO), interleukin (IL)-6, and tumor necrosis factor (TNF)-α in LPS-induced RAW264.7 cells at the same concentration.
31212928	6	60	theme	flavonoids	1854:1863	arg1	effects					1819:1825	the inhibitory effects	1804:1825	the inhibitory effects of total phenols and total flavonoids	1804:1863	These findings, combined with correlation analysis, showed that less-polar ginsenosides were major contributors to the inhibition of the overproduction of various inflammatory factors, while the inhibitory effects of total phenols and total flavonoids, and their antioxidant abilities, are also important.
31212928	6	60	theme	flavonoids	1854:1863	arg1	abilities					1888:1896	their antioxidant abilities	1870:1896	their antioxidant abilities	1870:1896	These findings, combined with correlation analysis, showed that less-polar ginsenosides were major contributors to the inhibition of the overproduction of various inflammatory factors, while the inhibitory effects of total phenols and total flavonoids, and their antioxidant abilities, are also important.
31212928	6	60	theme	flavonoids	1854:1863	arg1	important					1908:1916	important	1908:1916	important	1908:1916	These findings, combined with correlation analysis, showed that less-polar ginsenosides were major contributors to the inhibition of the overproduction of various inflammatory factors, while the inhibitory effects of total phenols and total flavonoids, and their antioxidant abilities, are also important.
31212928	1	61	from	changes	207:213	arg1	contents					238:245	contents	238:245	contents	238:245	We conducted this study for the first time to evaluate changes in the composition and contents of phenolic compounds and ginsenosides in ginseng shoot extracts (GSEs) prepared with different steaming times (2, 4, and 6 h) at 120 °C, as well as their antioxidant and anti-inflammatory activities in lipopolysaccharide (LPS)-induced RAW264.7 mouse macrophages (RAW264.7 cells).
31212928	1	61	from	changes	207:213	arg1	GSEs					313:316	GSEs	313:316	GSEs	313:316	We conducted this study for the first time to evaluate changes in the composition and contents of phenolic compounds and ginsenosides in ginseng shoot extracts (GSEs) prepared with different steaming times (2, 4, and 6 h) at 120 °C, as well as their antioxidant and anti-inflammatory activities in lipopolysaccharide (LPS)-induced RAW264.7 mouse macrophages (RAW264.7 cells).
31212928	1	61	from	changes	207:213	arg1	composition					222:232	the composition	218:232	the composition	218:232	We conducted this study for the first time to evaluate changes in the composition and contents of phenolic compounds and ginsenosides in ginseng shoot extracts (GSEs) prepared with different steaming times (2, 4, and 6 h) at 120 °C, as well as their antioxidant and anti-inflammatory activities in lipopolysaccharide (LPS)-induced RAW264.7 mouse macrophages (RAW264.7 cells).
31212928	1	61	from	changes	207:213	arg1	extracts					303:310	ginseng shoot extracts	289:310	ginseng shoot extracts (GSEs) prepared with different steaming times (2, 4, and 6 h) at 120 °C, as well as their antioxidant and anti-inflammatory activities in lipopolysaccharide (LPS)-induced RAW264.7 mouse macrophages (RAW264.7 cells)	289:525	We conducted this study for the first time to evaluate changes in the composition and contents of phenolic compounds and ginsenosides in ginseng shoot extracts (GSEs) prepared with different steaming times (2, 4, and 6 h) at 120 °C, as well as their antioxidant and anti-inflammatory activities in lipopolysaccharide (LPS)-induced RAW264.7 mouse macrophages (RAW264.7 cells).
31212928	4	62	theme	same	1337:1340	arg1	concentration					1342:1354	the same concentration	1333:1354	the same concentration	1333:1354	Furthermore, steamed GSEs exhibited a greater ability to inhibit the production of inflammatory mediators and pro-inflammatory cytokines, such as nitric oxide (NO), interleukin (IL)-6, and tumor necrosis factor (TNF)-α in LPS-induced RAW264.7 cells at the same concentration.
31212928	0	63	theme	Antioxidant	64:74	arg1	Capacities					98:107	Their Antioxidant and Anti-Inflammatory Capacities	58:107	Their Antioxidant and Anti-Inflammatory Capacities in LPS-Induced RAW264.7 Mouse Macrophages	58:149	Phenolic Compounds and Ginsenosides in Ginseng Shoots and Their Antioxidant and Anti-Inflammatory Capacities in LPS-Induced RAW264.7 Mouse Macrophages.
31212928	0	64	from	Ginsenosides	23:34	arg1	Shoots					47:52	Ginseng Shoots	39:52	Ginseng Shoots	39:52	Phenolic Compounds and Ginsenosides in Ginseng Shoots and Their Antioxidant and Anti-Inflammatory Capacities in LPS-Induced RAW264.7 Mouse Macrophages.
31212928	0	64	from	Ginsenosides	23:34	arg1	Macrophages					139:149	LPS-Induced RAW264.7 Mouse Macrophages	112:149	LPS-Induced RAW264.7 Mouse Macrophages	112:149	Phenolic Compounds and Ginsenosides in Ginseng Shoots and Their Antioxidant and Anti-Inflammatory Capacities in LPS-Induced RAW264.7 Mouse Macrophages.
31212928	4	65	theme	greater	1119:1125	arg1	ability					1127:1133	a greater ability	1117:1133	a greater ability to inhibit the production of inflammatory mediators and pro-inflammatory cytokines, such as nitric oxide (NO), interleukin (IL)-6, and tumor necrosis factor (TNF)-α in LPS-induced RAW264.7 cells at the same concentration	1117:1354	Furthermore, steamed GSEs exhibited a greater ability to inhibit the production of inflammatory mediators and pro-inflammatory cytokines, such as nitric oxide (NO), interleukin (IL)-6, and tumor necrosis factor (TNF)-α in LPS-induced RAW264.7 cells at the same concentration.
31212928	6	66	theme	total	1830:1834	arg1	phenols					1836:1842	total phenols	1830:1842	total phenols	1830:1842	These findings, combined with correlation analysis, showed that less-polar ginsenosides were major contributors to the inhibition of the overproduction of various inflammatory factors, while the inhibitory effects of total phenols and total flavonoids, and their antioxidant abilities, are also important.
31212928	0	67	theme	Anti-Inflammatory	80:96	arg1	Capacities					98:107	Their Antioxidant and Anti-Inflammatory Capacities	58:107	Their Antioxidant and Anti-Inflammatory Capacities in LPS-Induced RAW264.7 Mouse Macrophages	58:149	Phenolic Compounds and Ginsenosides in Ginseng Shoots and Their Antioxidant and Anti-Inflammatory Capacities in LPS-Induced RAW264.7 Mouse Macrophages.
31212928	1	68	theme	different	333:341	arg1	times					352:356	different steaming times	333:356	different steaming times (2, 4, and 6 h) at 120 °C	333:382	We conducted this study for the first time to evaluate changes in the composition and contents of phenolic compounds and ginsenosides in ginseng shoot extracts (GSEs) prepared with different steaming times (2, 4, and 6 h) at 120 °C, as well as their antioxidant and anti-inflammatory activities in lipopolysaccharide (LPS)-induced RAW264.7 mouse macrophages (RAW264.7 cells).
31212928	1	68	theme	different	333:341	arg1	h					371:371	2, 4, and 6 h	359:371	2, 4, and 6 h	359:371	We conducted this study for the first time to evaluate changes in the composition and contents of phenolic compounds and ginsenosides in ginseng shoot extracts (GSEs) prepared with different steaming times (2, 4, and 6 h) at 120 °C, as well as their antioxidant and anti-inflammatory activities in lipopolysaccharide (LPS)-induced RAW264.7 mouse macrophages (RAW264.7 cells).
31212928	3	69	theme	high-performance	849:864	arg1	HPLC					889:892	HPLC	889:892	HPLC	889:892	Among the 18 ginsenosides quantified using high-performance liquid chromatography (HPLC) with the aid of pure standards, polar ginsenosides were abundant in raw GSEs, whereas less-polar or rare ginsenosides appeared after steaming at 120 °C and increased with steaming time.
31212928	3	69	theme	high-performance	849:864	arg1	chromatography					873:886	high-performance liquid chromatography	849:886	high-performance liquid chromatography (HPLC)	849:893	Among the 18 ginsenosides quantified using high-performance liquid chromatography (HPLC) with the aid of pure standards, polar ginsenosides were abundant in raw GSEs, whereas less-polar or rare ginsenosides appeared after steaming at 120 °C and increased with steaming time.
31212928	0	70	from	Compounds	9:17	arg1	Shoots					47:52	Ginseng Shoots	39:52	Ginseng Shoots	39:52	Phenolic Compounds and Ginsenosides in Ginseng Shoots and Their Antioxidant and Anti-Inflammatory Capacities in LPS-Induced RAW264.7 Mouse Macrophages.
31212928	0	70	from	Compounds	9:17	arg1	Macrophages					139:149	LPS-Induced RAW264.7 Mouse Macrophages	112:149	LPS-Induced RAW264.7 Mouse Macrophages	112:149	Phenolic Compounds and Ginsenosides in Ginseng Shoots and Their Antioxidant and Anti-Inflammatory Capacities in LPS-Induced RAW264.7 Mouse Macrophages.
31212928	5	71	theme	oxide	1404:1408	arg1	iNOS					1420:1423	iNOS	1420:1423	iNOS	1420:1423	Relative expression levels of inducible nitric oxide synthase (iNOS), IL-6, TNF-α, and cyclooxygenase-2 (COX-2) mRNAs were attenuated by the GSEs, probably due to the enrichment of less-polar ginsenosides and enhanced antioxidant activity in steamed GSEs.
31212928	5	71	theme	oxide	1404:1408	arg1	synthase					1410:1417	inducible nitric oxide synthase	1387:1417	inducible nitric oxide synthase (iNOS)	1387:1424	Relative expression levels of inducible nitric oxide synthase (iNOS), IL-6, TNF-α, and cyclooxygenase-2 (COX-2) mRNAs were attenuated by the GSEs, probably due to the enrichment of less-polar ginsenosides and enhanced antioxidant activity in steamed GSEs.
31212928	4	72	theme	pro-inflammatory	1191:1206	arg1	IL					1259:1260	interleukin (IL)-6	1246:1263	interleukin (IL)-6	1246:1263	Furthermore, steamed GSEs exhibited a greater ability to inhibit the production of inflammatory mediators and pro-inflammatory cytokines, such as nitric oxide (NO), interleukin (IL)-6, and tumor necrosis factor (TNF)-α in LPS-induced RAW264.7 cells at the same concentration.
31212928	4	72	theme	pro-inflammatory	1191:1206	arg1	-α					1297:1298	tumor necrosis factor (TNF)-α	1270:1298	tumor necrosis factor (TNF)-α	1270:1298	Furthermore, steamed GSEs exhibited a greater ability to inhibit the production of inflammatory mediators and pro-inflammatory cytokines, such as nitric oxide (NO), interleukin (IL)-6, and tumor necrosis factor (TNF)-α in LPS-induced RAW264.7 cells at the same concentration.
31212928	4	72	theme	pro-inflammatory	1191:1206	arg1	oxide					1234:1238	nitric oxide	1227:1238	nitric oxide (NO)	1227:1243	Furthermore, steamed GSEs exhibited a greater ability to inhibit the production of inflammatory mediators and pro-inflammatory cytokines, such as nitric oxide (NO), interleukin (IL)-6, and tumor necrosis factor (TNF)-α in LPS-induced RAW264.7 cells at the same concentration.
31212928	4	72	theme	pro-inflammatory	1191:1206	arg1	cytokines					1208:1216	pro-inflammatory cytokines	1191:1216	pro-inflammatory cytokines	1191:1216	Furthermore, steamed GSEs exhibited a greater ability to inhibit the production of inflammatory mediators and pro-inflammatory cytokines, such as nitric oxide (NO), interleukin (IL)-6, and tumor necrosis factor (TNF)-α in LPS-induced RAW264.7 cells at the same concentration.
31212928	4	73	theme	RAW264.7	1315:1322	arg1	cells					1324:1328	LPS-induced RAW264.7 cells	1303:1328	LPS-induced RAW264.7 cells	1303:1328	Furthermore, steamed GSEs exhibited a greater ability to inhibit the production of inflammatory mediators and pro-inflammatory cytokines, such as nitric oxide (NO), interleukin (IL)-6, and tumor necrosis factor (TNF)-α in LPS-induced RAW264.7 cells at the same concentration.
31212928	2	74	dep	2,2'-diphenyl-1-picrylhydrazyl	678:707	arg1	capacities					794:803	scavenging capacities	783:803	scavenging capacities	783:803	The results show that total phenol and flavonoid contents were both significantly higher in steamed versus raw GSEs, and the same trend was found for 2,2'-diphenyl-1-picrylhydrazyl (DPPH•) and 2,2'-azobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS•+) scavenging capacities.
31212928	1	75	theme	mouse	492:496	arg1	macrophages					498:508	-induced RAW264.7 mouse macrophages	474:508	lipopolysaccharide (LPS)-induced RAW264.7 mouse macrophages (RAW264.7 cells)	450:525	We conducted this study for the first time to evaluate changes in the composition and contents of phenolic compounds and ginsenosides in ginseng shoot extracts (GSEs) prepared with different steaming times (2, 4, and 6 h) at 120 °C, as well as their antioxidant and anti-inflammatory activities in lipopolysaccharide (LPS)-induced RAW264.7 mouse macrophages (RAW264.7 cells).
31212928	1	75	theme	mouse	492:496	arg1	cells					520:524	RAW264.7 cells	511:524	RAW264.7 cells	511:524	We conducted this study for the first time to evaluate changes in the composition and contents of phenolic compounds and ginsenosides in ginseng shoot extracts (GSEs) prepared with different steaming times (2, 4, and 6 h) at 120 °C, as well as their antioxidant and anti-inflammatory activities in lipopolysaccharide (LPS)-induced RAW264.7 mouse macrophages (RAW264.7 cells).
31212928	0	76	theme	RAW264.7	124:131	arg1	Macrophages					139:149	LPS-Induced RAW264.7 Mouse Macrophages	112:149	LPS-Induced RAW264.7 Mouse Macrophages	112:149	Phenolic Compounds and Ginsenosides in Ginseng Shoots and Their Antioxidant and Anti-Inflammatory Capacities in LPS-Induced RAW264.7 Mouse Macrophages.
31212928	2	77	theme	raw	635:637	arg1	GSEs					639:642	steamed versus raw GSEs	620:642	steamed versus raw GSEs	620:642	The results show that total phenol and flavonoid contents were both significantly higher in steamed versus raw GSEs, and the same trend was found for 2,2'-diphenyl-1-picrylhydrazyl (DPPH•) and 2,2'-azobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS•+) scavenging capacities.
31212928	5	78	theme	antioxidant	1575:1585	arg1	activity					1587:1594	enhanced antioxidant activity	1566:1594	enhanced antioxidant activity in steamed GSEs	1566:1610	Relative expression levels of inducible nitric oxide synthase (iNOS), IL-6, TNF-α, and cyclooxygenase-2 (COX-2) mRNAs were attenuated by the GSEs, probably due to the enrichment of less-polar ginsenosides and enhanced antioxidant activity in steamed GSEs.
31212928	5	79	theme	inducible	1387:1395	arg1	iNOS					1420:1423	iNOS	1420:1423	iNOS	1420:1423	Relative expression levels of inducible nitric oxide synthase (iNOS), IL-6, TNF-α, and cyclooxygenase-2 (COX-2) mRNAs were attenuated by the GSEs, probably due to the enrichment of less-polar ginsenosides and enhanced antioxidant activity in steamed GSEs.
31212928	5	79	theme	inducible	1387:1395	arg1	synthase					1410:1417	inducible nitric oxide synthase	1387:1417	inducible nitric oxide synthase (iNOS)	1387:1424	Relative expression levels of inducible nitric oxide synthase (iNOS), IL-6, TNF-α, and cyclooxygenase-2 (COX-2) mRNAs were attenuated by the GSEs, probably due to the enrichment of less-polar ginsenosides and enhanced antioxidant activity in steamed GSEs.
31212928	2	80	theme	steamed	620:626	arg1	GSEs					639:642	steamed versus raw GSEs	620:642	steamed versus raw GSEs	620:642	The results show that total phenol and flavonoid contents were both significantly higher in steamed versus raw GSEs, and the same trend was found for 2,2'-diphenyl-1-picrylhydrazyl (DPPH•) and 2,2'-azobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS•+) scavenging capacities.
31212928	6	81	theme	antioxidant	1876:1886	arg1	abilities					1888:1896	their antioxidant abilities	1870:1896	their antioxidant abilities	1870:1896	These findings, combined with correlation analysis, showed that less-polar ginsenosides were major contributors to the inhibition of the overproduction of various inflammatory factors, while the inhibitory effects of total phenols and total flavonoids, and their antioxidant abilities, are also important.
31212928	1	82	theme	ginseng	289:295	arg1	GSEs					313:316	GSEs	313:316	GSEs	313:316	We conducted this study for the first time to evaluate changes in the composition and contents of phenolic compounds and ginsenosides in ginseng shoot extracts (GSEs) prepared with different steaming times (2, 4, and 6 h) at 120 °C, as well as their antioxidant and anti-inflammatory activities in lipopolysaccharide (LPS)-induced RAW264.7 mouse macrophages (RAW264.7 cells).
31212928	1	82	theme	ginseng	289:295	arg1	extracts					303:310	ginseng shoot extracts	289:310	ginseng shoot extracts (GSEs) prepared with different steaming times (2, 4, and 6 h) at 120 °C, as well as their antioxidant and anti-inflammatory activities in lipopolysaccharide (LPS)-induced RAW264.7 mouse macrophages (RAW264.7 cells)	289:525	We conducted this study for the first time to evaluate changes in the composition and contents of phenolic compounds and ginsenosides in ginseng shoot extracts (GSEs) prepared with different steaming times (2, 4, and 6 h) at 120 °C, as well as their antioxidant and anti-inflammatory activities in lipopolysaccharide (LPS)-induced RAW264.7 mouse macrophages (RAW264.7 cells).
31212928	3	83	theme	steaming	1066:1073	arg1	time					1075:1078	steaming time	1066:1078	steaming time	1066:1078	Among the 18 ginsenosides quantified using high-performance liquid chromatography (HPLC) with the aid of pure standards, polar ginsenosides were abundant in raw GSEs, whereas less-polar or rare ginsenosides appeared after steaming at 120 °C and increased with steaming time.
31212928	6	84	theme	less-polar	1677:1686	arg1	ginsenosides					1688:1699	less-polar ginsenosides	1677:1699	less-polar ginsenosides	1677:1699	These findings, combined with correlation analysis, showed that less-polar ginsenosides were major contributors to the inhibition of the overproduction of various inflammatory factors, while the inhibitory effects of total phenols and total flavonoids, and their antioxidant abilities, are also important.
31212928	5	85	theme	TNF-α	1433:1437	arg1	levels					1377:1382	Relative expression levels	1357:1382	Relative expression levels of inducible nitric oxide synthase (iNOS), IL-6, TNF-α, and cyclooxygenase-2 (COX-2) mRNAs	1357:1473	Relative expression levels of inducible nitric oxide synthase (iNOS), IL-6, TNF-α, and cyclooxygenase-2 (COX-2) mRNAs were attenuated by the GSEs, probably due to the enrichment of less-polar ginsenosides and enhanced antioxidant activity in steamed GSEs.
31212928	5	86	theme	less-polar	1538:1547	arg1	ginsenosides					1549:1560	less-polar ginsenosides	1538:1560	less-polar ginsenosides	1538:1560	Relative expression levels of inducible nitric oxide synthase (iNOS), IL-6, TNF-α, and cyclooxygenase-2 (COX-2) mRNAs were attenuated by the GSEs, probably due to the enrichment of less-polar ginsenosides and enhanced antioxidant activity in steamed GSEs.
31212928	0	87	theme	Ginseng	39:45	arg1	Shoots					47:52	Ginseng Shoots	39:52	Ginseng Shoots	39:52	Phenolic Compounds and Ginsenosides in Ginseng Shoots and Their Antioxidant and Anti-Inflammatory Capacities in LPS-Induced RAW264.7 Mouse Macrophages.
31212928	1	88	from	activities	436:445	arg1	lipopolysaccharide					450:467	lipopolysaccharide	450:467	lipopolysaccharide (LPS)-induced RAW264.7 mouse macrophages (RAW264.7 cells)	450:525	We conducted this study for the first time to evaluate changes in the composition and contents of phenolic compounds and ginsenosides in ginseng shoot extracts (GSEs) prepared with different steaming times (2, 4, and 6 h) at 120 °C, as well as their antioxidant and anti-inflammatory activities in lipopolysaccharide (LPS)-induced RAW264.7 mouse macrophages (RAW264.7 cells).
31212928	1	88	from	activities	436:445	arg1	LPS					470:472	LPS	470:472	LPS	470:472	We conducted this study for the first time to evaluate changes in the composition and contents of phenolic compounds and ginsenosides in ginseng shoot extracts (GSEs) prepared with different steaming times (2, 4, and 6 h) at 120 °C, as well as their antioxidant and anti-inflammatory activities in lipopolysaccharide (LPS)-induced RAW264.7 mouse macrophages (RAW264.7 cells).
31212928	3	89	theme	pure	911:914	arg1	standards					916:924	pure standards	911:924	pure standards	911:924	Among the 18 ginsenosides quantified using high-performance liquid chromatography (HPLC) with the aid of pure standards, polar ginsenosides were abundant in raw GSEs, whereas less-polar or rare ginsenosides appeared after steaming at 120 °C and increased with steaming time.
31212928	6	90	theme	overproduction	1750:1763	arg1	inhibition					1732:1741	the inhibition	1728:1741	the inhibition of the overproduction of various inflammatory factors	1728:1795	These findings, combined with correlation analysis, showed that less-polar ginsenosides were major contributors to the inhibition of the overproduction of various inflammatory factors, while the inhibitory effects of total phenols and total flavonoids, and their antioxidant abilities, are also important.
31212928	5	91	theme	IL-6	1427:1430	arg1	levels					1377:1382	Relative expression levels	1357:1382	Relative expression levels of inducible nitric oxide synthase (iNOS), IL-6, TNF-α, and cyclooxygenase-2 (COX-2) mRNAs	1357:1473	Relative expression levels of inducible nitric oxide synthase (iNOS), IL-6, TNF-α, and cyclooxygenase-2 (COX-2) mRNAs were attenuated by the GSEs, probably due to the enrichment of less-polar ginsenosides and enhanced antioxidant activity in steamed GSEs.
31212928	2	92	theme	3-ethylbenzothiazoline-6-sulfonic	734:766	arg1	2,2'-azobis-					721:732	2,2'-azobis-	721:732	2,2'-azobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS•+)	721:781	The results show that total phenol and flavonoid contents were both significantly higher in steamed versus raw GSEs, and the same trend was found for 2,2'-diphenyl-1-picrylhydrazyl (DPPH•) and 2,2'-azobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS•+) scavenging capacities.
31212928	2	92	theme	3-ethylbenzothiazoline-6-sulfonic	734:766	arg1	acid					768:771	3-ethylbenzothiazoline-6-sulfonic acid	734:771	3-ethylbenzothiazoline-6-sulfonic acid	734:771	The results show that total phenol and flavonoid contents were both significantly higher in steamed versus raw GSEs, and the same trend was found for 2,2'-diphenyl-1-picrylhydrazyl (DPPH•) and 2,2'-azobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS•+) scavenging capacities.
31212928	1	93	theme	-induced	474:481	arg1	macrophages					498:508	-induced RAW264.7 mouse macrophages	474:508	lipopolysaccharide (LPS)-induced RAW264.7 mouse macrophages (RAW264.7 cells)	450:525	We conducted this study for the first time to evaluate changes in the composition and contents of phenolic compounds and ginsenosides in ginseng shoot extracts (GSEs) prepared with different steaming times (2, 4, and 6 h) at 120 °C, as well as their antioxidant and anti-inflammatory activities in lipopolysaccharide (LPS)-induced RAW264.7 mouse macrophages (RAW264.7 cells).
31212928	1	93	theme	-induced	474:481	arg1	cells					520:524	RAW264.7 cells	511:524	RAW264.7 cells	511:524	We conducted this study for the first time to evaluate changes in the composition and contents of phenolic compounds and ginsenosides in ginseng shoot extracts (GSEs) prepared with different steaming times (2, 4, and 6 h) at 120 °C, as well as their antioxidant and anti-inflammatory activities in lipopolysaccharide (LPS)-induced RAW264.7 mouse macrophages (RAW264.7 cells).
30556368	2	0	dep	forages	548:554	arg1	starch					561:566	starch	561:566	starch	561:566	A total of 20 Holstein cows were assigned to 1 of 2 treatment diets composed of either mixed forages (MF, starch : 21.50%) or corn stover forage (CS, starch : 25.39%).
30556368	2	0	dep	forages	548:554	arg1	MF					557:558	MF	557:558	MF	557:558	A total of 20 Holstein cows were assigned to 1 of 2 treatment diets composed of either mixed forages (MF, starch : 21.50%) or corn stover forage (CS, starch : 25.39%).
30556368	1	1	from	enzymes	432:438	arg1	cows					449:452	dairy cows	443:452	dairy cows	443:452	The aim of this study was to determine the effects of isonitrogenous and isocaloric diets containing different qualities of forages and concentrate content on milk fat composition and genes that encode mammary lipogenic enzymes in dairy cows.
30556368	0	2	theme	lipogenic	165:173	arg1	enzymes					175:181	mammary lipogenic enzymes	157:181	mammary lipogenic enzymes	157:181	Effects of isonitrogenous and isocaloric total mixed ration composed of forages with different quality on milk fatty acid composition and gene expression of mammary lipogenic enzymes in mid-lactating dairy cows.
30556368	0	3	from	expression	143:152	arg1	cows					206:209	mid-lactating dairy cows	186:209	mid-lactating dairy cows	186:209	Effects of isonitrogenous and isocaloric total mixed ration composed of forages with different quality on milk fatty acid composition and gene expression of mammary lipogenic enzymes in mid-lactating dairy cows.
30556368	3	4	theme	tissue	631:636	arg1	biopsies					638:645	Mammary tissue biopsies	623:645	Mammary tissue biopsies	623:645	Mammary tissue biopsies were performed to analyze the mRNA expression of lipogenic enzymes.
30556368	1	5	theme	dairy	443:447	arg1	cows					449:452	dairy cows	443:452	dairy cows	443:452	The aim of this study was to determine the effects of isonitrogenous and isocaloric diets containing different qualities of forages and concentrate content on milk fat composition and genes that encode mammary lipogenic enzymes in dairy cows.
30556368	7	6	theme	nuclear	1198:1204	arg1	factors					1220:1226	the nuclear transcription factors	1194:1226	the nuclear transcription factors	1194:1226	No effects on the nuclear transcription factors were observed between the two treatments.
30556368	0	7	theme	dairy	200:204	arg1	cows					206:209	mid-lactating dairy cows	186:209	mid-lactating dairy cows	186:209	Effects of isonitrogenous and isocaloric total mixed ration composed of forages with different quality on milk fatty acid composition and gene expression of mammary lipogenic enzymes in mid-lactating dairy cows.
30556368	6	8	theme	de	1056:1057	arg1	synthesis					1067:1075	de novo FA synthesis	1056:1075	de novo FA synthesis	1056:1075	Genes involved in de novo FA synthesis, FA uptake and transport, and Δ9-desaturation were lower in the CS treatment than in the MF treatment.
30556368	1	9	contain	containing	302:311	arg1	diets					296:300	isonitrogenous and isocaloric diets	266:300	isonitrogenous and isocaloric diets containing different qualities of forages and concentrate content	266:366	The aim of this study was to determine the effects of isonitrogenous and isocaloric diets containing different qualities of forages and concentrate content on milk fat composition and genes that encode mammary lipogenic enzymes in dairy cows.
30556368	1	9	contain	containing	302:311	arg2	qualities					323:331	different qualities	313:331	different qualities of forages and concentrate content	313:366	The aim of this study was to determine the effects of isonitrogenous and isocaloric diets containing different qualities of forages and concentrate content on milk fat composition and genes that encode mammary lipogenic enzymes in dairy cows.
30556368	0	10	theme	fatty	111:115	arg1	composition					122:132	milk fatty acid composition	106:132	milk fatty acid composition	106:132	Effects of isonitrogenous and isocaloric total mixed ration composed of forages with different quality on milk fatty acid composition and gene expression of mammary lipogenic enzymes in mid-lactating dairy cows.
30556368	1	11	theme	fat	376:378	arg1	composition					380:390	milk fat composition	371:390	milk fat composition	371:390	The aim of this study was to determine the effects of isonitrogenous and isocaloric diets containing different qualities of forages and concentrate content on milk fat composition and genes that encode mammary lipogenic enzymes in dairy cows.
30556368	2	12	dep	MF	557:558	arg1	%					575:575	21.50%	570:575	21.50%	570:575	A total of 20 Holstein cows were assigned to 1 of 2 treatment diets composed of either mixed forages (MF, starch : 21.50%) or corn stover forage (CS, starch : 25.39%).
30556368	2	13	theme	treatment	507:515	arg1	diets					517:521	1 of 2 treatment diets	500:521	1 of 2 treatment diets composed of either mixed forages (MF, starch : 21.50%) or corn stover forage (CS, starch : 25.39%)	500:620	A total of 20 Holstein cows were assigned to 1 of 2 treatment diets composed of either mixed forages (MF, starch : 21.50%) or corn stover forage (CS, starch : 25.39%).
30556368	0	14	theme	gene	138:141	arg1	expression					143:152	gene expression	138:152	gene expression of mammary lipogenic enzymes in mid-lactating dairy cows	138:209	Effects of isonitrogenous and isocaloric total mixed ration composed of forages with different quality on milk fatty acid composition and gene expression of mammary lipogenic enzymes in mid-lactating dairy cows.
30556368	6	15	theme	FA	1078:1079	arg1	uptake					1081:1086	FA uptake	1078:1086	FA uptake	1078:1086	Genes involved in de novo FA synthesis, FA uptake and transport, and Δ9-desaturation were lower in the CS treatment than in the MF treatment.
30556368	2	16	dep	CS	601:602	arg1	%					619:619	25.39%	614:619	25.39%	614:619	A total of 20 Holstein cows were assigned to 1 of 2 treatment diets composed of either mixed forages (MF, starch : 21.50%) or corn stover forage (CS, starch : 25.39%).
30556368	1	17	theme	mammary	414:420	arg1	enzymes					432:438	mammary lipogenic enzymes	414:438	mammary lipogenic enzymes in dairy cows	414:452	The aim of this study was to determine the effects of isonitrogenous and isocaloric diets containing different qualities of forages and concentrate content on milk fat composition and genes that encode mammary lipogenic enzymes in dairy cows.
30556368	1	17	theme	mammary	414:420	arg1	genes					396:400	genes	396:400	genes that encode mammary lipogenic enzymes in dairy cows	396:452	The aim of this study was to determine the effects of isonitrogenous and isocaloric diets containing different qualities of forages and concentrate content on milk fat composition and genes that encode mammary lipogenic enzymes in dairy cows.
30556368	5	18	theme	yield	815:819	arg1	contents					911:918	The milk yield, fat content and saturated fatty acid (SFA) and short- and medium-chain fatty acid (SMFA) contents	806:918	The milk yield, fat content and saturated fatty acid (SFA) and short- and medium-chain fatty acid (SMFA) contents in milk	806:926	The milk yield, fat content and saturated fatty acid (SFA) and short- and medium-chain fatty acid (SMFA) contents in milk were lower in the CS diet than in the MF diet, but the unsaturated FA and long-chain FA contents were higher.
30556368	3	19	theme	lipogenic	696:704	arg1	enzymes					706:712	lipogenic enzymes	696:712	lipogenic enzymes	696:712	Mammary tissue biopsies were performed to analyze the mRNA expression of lipogenic enzymes.
30556368	5	20	from	contents	911:918	arg1	milk					923:926	milk	923:926	milk	923:926	The milk yield, fat content and saturated fatty acid (SFA) and short- and medium-chain fatty acid (SMFA) contents in milk were lower in the CS diet than in the MF diet, but the unsaturated FA and long-chain FA contents were higher.
30556368	2	21	theme	Holstein	469:476	arg1	cows					478:481	20 Holstein cows	466:481	20 Holstein cows	466:481	A total of 20 Holstein cows were assigned to 1 of 2 treatment diets composed of either mixed forages (MF, starch : 21.50%) or corn stover forage (CS, starch : 25.39%).
30556368	1	22	theme	forages	336:342	arg1	qualities					323:331	different qualities	313:331	different qualities of forages and concentrate content	313:366	The aim of this study was to determine the effects of isonitrogenous and isocaloric diets containing different qualities of forages and concentrate content on milk fat composition and genes that encode mammary lipogenic enzymes in dairy cows.
30556368	8	23	theme	corn	1294:1297	arg1	diet					1306:1309	corn stover diet	1294:1309	corn stover diet	1294:1309	The data indicated that corn stover diet reduced the milk yield, fat content, SMFA, and SFA contents in milk, as well as the gene expression of mammary lipogenic enzymes in dairy cows.
30556368	0	24	theme	different	85:93	arg1	quality					95:101	different quality	85:101	different quality on milk fatty acid composition and gene expression of mammary lipogenic enzymes in mid-lactating dairy cows	85:209	Effects of isonitrogenous and isocaloric total mixed ration composed of forages with different quality on milk fatty acid composition and gene expression of mammary lipogenic enzymes in mid-lactating dairy cows.
30556368	1	25	theme	concentrate	348:358	arg1	content					360:366	concentrate content	348:366	concentrate content	348:366	The aim of this study was to determine the effects of isonitrogenous and isocaloric diets containing different qualities of forages and concentrate content on milk fat composition and genes that encode mammary lipogenic enzymes in dairy cows.
30556368	5	26	theme	fatty	848:852	arg1	SFA					860:862	SFA	860:862	SFA	860:862	The milk yield, fat content and saturated fatty acid (SFA) and short- and medium-chain fatty acid (SMFA) contents in milk were lower in the CS diet than in the MF diet, but the unsaturated FA and long-chain FA contents were higher.
30556368	5	26	theme	fatty	848:852	arg1	acid					854:857	saturated fatty acid	838:857	saturated fatty acid (SFA)	838:863	The milk yield, fat content and saturated fatty acid (SFA) and short- and medium-chain fatty acid (SMFA) contents in milk were lower in the CS diet than in the MF diet, but the unsaturated FA and long-chain FA contents were higher.
30556368	5	26	theme	fatty	848:852	arg1	SMFA					905:908	SMFA	905:908	SMFA	905:908	The milk yield, fat content and saturated fatty acid (SFA) and short- and medium-chain fatty acid (SMFA) contents in milk were lower in the CS diet than in the MF diet, but the unsaturated FA and long-chain FA contents were higher.
30556368	6	27	theme	CS	1141:1142	arg1	treatment					1144:1152	the CS treatment	1137:1152	the CS treatment	1137:1152	Genes involved in de novo FA synthesis, FA uptake and transport, and Δ9-desaturation were lower in the CS treatment than in the MF treatment.
30556368	5	28	theme	CS	946:947	arg1	diet					949:952	the CS diet	942:952	the CS diet	942:952	The milk yield, fat content and saturated fatty acid (SFA) and short- and medium-chain fatty acid (SMFA) contents in milk were lower in the CS diet than in the MF diet, but the unsaturated FA and long-chain FA contents were higher.
30556368	5	29	theme	unsaturated	983:993	arg1	FA					995:996	the unsaturated FA and long-chain FA contents	979:1023	FA	995:996	The milk yield, fat content and saturated fatty acid (SFA) and short- and medium-chain fatty acid (SMFA) contents in milk were lower in the CS diet than in the MF diet, but the unsaturated FA and long-chain FA contents were higher.
30556368	5	29	theme	unsaturated	983:993	arg1	higher					1030:1035	higher	1030:1035	higher	1030:1035	The milk yield, fat content and saturated fatty acid (SFA) and short- and medium-chain fatty acid (SMFA) contents in milk were lower in the CS diet than in the MF diet, but the unsaturated FA and long-chain FA contents were higher.
30556368	5	30	theme	fat	822:824	arg1	content					826:832	fat content	822:832	fat content	822:832	The milk yield, fat content and saturated fatty acid (SFA) and short- and medium-chain fatty acid (SMFA) contents in milk were lower in the CS diet than in the MF diet, but the unsaturated FA and long-chain FA contents were higher.
30556368	8	31	theme	yield	1328:1332	arg1	contents					1362:1369	the milk yield, fat content, SMFA, and SFA contents	1319:1369	the milk yield, fat content, SMFA, and SFA contents in milk	1319:1377	The data indicated that corn stover diet reduced the milk yield, fat content, SMFA, and SFA contents in milk, as well as the gene expression of mammary lipogenic enzymes in dairy cows.
30556368	0	32	theme	isonitrogenous	11:24	arg1	ration					53:58	isonitrogenous and isocaloric total mixed ration	11:58	isonitrogenous and isocaloric total mixed ration	11:58	Effects of isonitrogenous and isocaloric total mixed ration composed of forages with different quality on milk fatty acid composition and gene expression of mammary lipogenic enzymes in mid-lactating dairy cows.
30556368	1	33	theme	isonitrogenous	266:279	arg1	diets					296:300	isonitrogenous and isocaloric diets	266:300	isonitrogenous and isocaloric diets containing different qualities of forages and concentrate content	266:366	The aim of this study was to determine the effects of isonitrogenous and isocaloric diets containing different qualities of forages and concentrate content on milk fat composition and genes that encode mammary lipogenic enzymes in dairy cows.
30556368	8	34	theme	fat	1335:1337	arg1	content					1339:1345	fat content	1335:1345	fat content	1335:1345	The data indicated that corn stover diet reduced the milk yield, fat content, SMFA, and SFA contents in milk, as well as the gene expression of mammary lipogenic enzymes in dairy cows.
30556368	0	35	theme	isocaloric	30:39	arg1	ration					53:58	isonitrogenous and isocaloric total mixed ration	11:58	isonitrogenous and isocaloric total mixed ration	11:58	Effects of isonitrogenous and isocaloric total mixed ration composed of forages with different quality on milk fatty acid composition and gene expression of mammary lipogenic enzymes in mid-lactating dairy cows.
30556368	1	36	theme	isocaloric	285:294	arg1	diets					296:300	isonitrogenous and isocaloric diets	266:300	isonitrogenous and isocaloric diets containing different qualities of forages and concentrate content	266:366	The aim of this study was to determine the effects of isonitrogenous and isocaloric diets containing different qualities of forages and concentrate content on milk fat composition and genes that encode mammary lipogenic enzymes in dairy cows.
30556368	5	37	theme	medium-chain	880:891	arg1	acid					899:902	short- and medium-chain fatty acid	869:902	short- and medium-chain fatty acid	869:902	The milk yield, fat content and saturated fatty acid (SFA) and short- and medium-chain fatty acid (SMFA) contents in milk were lower in the CS diet than in the MF diet, but the unsaturated FA and long-chain FA contents were higher.
30556368	4	38	theme	body	734:737	arg1	weight					739:744	body weight	734:744	body weight	734:744	Dry matter intake, body weight, milk protein, and lactose were not affected by treatments.
30556368	0	39	theme	mixed	47:51	arg1	ration					53:58	isonitrogenous and isocaloric total mixed ration	11:58	isonitrogenous and isocaloric total mixed ration	11:58	Effects of isonitrogenous and isocaloric total mixed ration composed of forages with different quality on milk fatty acid composition and gene expression of mammary lipogenic enzymes in mid-lactating dairy cows.
30556368	6	40	theme	FA	1064:1065	arg1	synthesis					1067:1075	de novo FA synthesis	1056:1075	de novo FA synthesis	1056:1075	Genes involved in de novo FA synthesis, FA uptake and transport, and Δ9-desaturation were lower in the CS treatment than in the MF treatment.
30556368	8	41	theme	SFA	1358:1360	arg1	contents					1362:1369	the milk yield, fat content, SMFA, and SFA contents	1319:1369	the milk yield, fat content, SMFA, and SFA contents in milk	1319:1377	The data indicated that corn stover diet reduced the milk yield, fat content, SMFA, and SFA contents in milk, as well as the gene expression of mammary lipogenic enzymes in dairy cows.
30556368	4	42	theme	Dry	715:717	arg1	intake					726:731	Dry matter intake	715:731	Dry matter intake	715:731	Dry matter intake, body weight, milk protein, and lactose were not affected by treatments.
30556368	6	43	dep	de	1056:1057	arg1	novo					1059:1062	novo	1059:1062	novo	1059:1062	Genes involved in de novo FA synthesis, FA uptake and transport, and Δ9-desaturation were lower in the CS treatment than in the MF treatment.
30556368	5	44	theme	short-	869:874	arg1	acid					899:902	short- and medium-chain fatty acid	869:902	short- and medium-chain fatty acid	869:902	The milk yield, fat content and saturated fatty acid (SFA) and short- and medium-chain fatty acid (SMFA) contents in milk were lower in the CS diet than in the MF diet, but the unsaturated FA and long-chain FA contents were higher.
30556368	0	45	theme	enzymes	175:181	arg1	composition					122:132	milk fatty acid composition	106:132	milk fatty acid composition	106:132	Effects of isonitrogenous and isocaloric total mixed ration composed of forages with different quality on milk fatty acid composition and gene expression of mammary lipogenic enzymes in mid-lactating dairy cows.
30556368	0	45	theme	enzymes	175:181	arg1	expression					143:152	gene expression	138:152	gene expression of mammary lipogenic enzymes in mid-lactating dairy cows	138:209	Effects of isonitrogenous and isocaloric total mixed ration composed of forages with different quality on milk fatty acid composition and gene expression of mammary lipogenic enzymes in mid-lactating dairy cows.
30556368	8	46	theme	mammary	1414:1420	arg1	enzymes					1432:1438	mammary lipogenic enzymes	1414:1438	mammary lipogenic enzymes	1414:1438	The data indicated that corn stover diet reduced the milk yield, fat content, SMFA, and SFA contents in milk, as well as the gene expression of mammary lipogenic enzymes in dairy cows.
30556368	5	47	theme	FA	1013:1014	arg1	contents					1016:1023	the unsaturated FA and long-chain FA contents	979:1023	contents	1016:1023	The milk yield, fat content and saturated fatty acid (SFA) and short- and medium-chain fatty acid (SMFA) contents in milk were lower in the CS diet than in the MF diet, but the unsaturated FA and long-chain FA contents were higher.
30556368	0	48	theme	mid-lactating	186:198	arg1	cows					206:209	mid-lactating dairy cows	186:209	mid-lactating dairy cows	186:209	Effects of isonitrogenous and isocaloric total mixed ration composed of forages with different quality on milk fatty acid composition and gene expression of mammary lipogenic enzymes in mid-lactating dairy cows.
30556368	0	49	with	forages	72:78	arg1	quality					95:101	different quality	85:101	different quality on milk fatty acid composition and gene expression of mammary lipogenic enzymes in mid-lactating dairy cows	85:209	Effects of isonitrogenous and isocaloric total mixed ration composed of forages with different quality on milk fatty acid composition and gene expression of mammary lipogenic enzymes in mid-lactating dairy cows.
30556368	8	50	theme	enzymes	1432:1438	arg1	expression					1400:1409	the gene expression	1391:1409	the gene expression of mammary lipogenic enzymes in dairy cows	1391:1452	The data indicated that corn stover diet reduced the milk yield, fat content, SMFA, and SFA contents in milk, as well as the gene expression of mammary lipogenic enzymes in dairy cows.
30556368	8	50	theme	enzymes	1432:1438	arg1	contents					1362:1369	the milk yield, fat content, SMFA, and SFA contents	1319:1369	the milk yield, fat content, SMFA, and SFA contents in milk	1319:1377	The data indicated that corn stover diet reduced the milk yield, fat content, SMFA, and SFA contents in milk, as well as the gene expression of mammary lipogenic enzymes in dairy cows.
30556368	8	51	theme	dairy	1443:1447	arg1	cows					1449:1452	dairy cows	1443:1452	dairy cows	1443:1452	The data indicated that corn stover diet reduced the milk yield, fat content, SMFA, and SFA contents in milk, as well as the gene expression of mammary lipogenic enzymes in dairy cows.
30556368	7	52	theme	transcription	1206:1218	arg1	factors					1220:1226	the nuclear transcription factors	1194:1226	the nuclear transcription factors	1194:1226	No effects on the nuclear transcription factors were observed between the two treatments.
30556368	3	53	theme	Mammary	623:629	arg1	biopsies					638:645	Mammary tissue biopsies	623:645	Mammary tissue biopsies	623:645	Mammary tissue biopsies were performed to analyze the mRNA expression of lipogenic enzymes.
30556368	1	54	from	effects	255:261	arg1	enzymes					432:438	mammary lipogenic enzymes	414:438	mammary lipogenic enzymes in dairy cows	414:452	The aim of this study was to determine the effects of isonitrogenous and isocaloric diets containing different qualities of forages and concentrate content on milk fat composition and genes that encode mammary lipogenic enzymes in dairy cows.
30556368	1	54	from	effects	255:261	arg1	genes					396:400	genes	396:400	genes that encode mammary lipogenic enzymes in dairy cows	396:452	The aim of this study was to determine the effects of isonitrogenous and isocaloric diets containing different qualities of forages and concentrate content on milk fat composition and genes that encode mammary lipogenic enzymes in dairy cows.
30556368	1	54	from	effects	255:261	arg1	composition					380:390	milk fat composition	371:390	milk fat composition	371:390	The aim of this study was to determine the effects of isonitrogenous and isocaloric diets containing different qualities of forages and concentrate content on milk fat composition and genes that encode mammary lipogenic enzymes in dairy cows.
30556368	4	55	theme	milk	747:750	arg1	protein					752:758	milk protein	747:758	milk protein	747:758	Dry matter intake, body weight, milk protein, and lactose were not affected by treatments.
30556368	0	56	theme	acid	117:120	arg1	composition					122:132	milk fatty acid composition	106:132	milk fatty acid composition	106:132	Effects of isonitrogenous and isocaloric total mixed ration composed of forages with different quality on milk fatty acid composition and gene expression of mammary lipogenic enzymes in mid-lactating dairy cows.
30556368	8	57	from	contents	1362:1369	arg1	milk					1374:1377	milk	1374:1377	milk	1374:1377	The data indicated that corn stover diet reduced the milk yield, fat content, SMFA, and SFA contents in milk, as well as the gene expression of mammary lipogenic enzymes in dairy cows.
30556368	8	57	from	contents	1362:1369	arg1	cows					1449:1452	dairy cows	1443:1452	dairy cows	1443:1452	The data indicated that corn stover diet reduced the milk yield, fat content, SMFA, and SFA contents in milk, as well as the gene expression of mammary lipogenic enzymes in dairy cows.
30556368	2	58	theme	mixed	542:546	arg1	forages					548:554	mixed forages	542:554	mixed forages (MF, starch : 21.50%)	542:576	A total of 20 Holstein cows were assigned to 1 of 2 treatment diets composed of either mixed forages (MF, starch : 21.50%) or corn stover forage (CS, starch : 25.39%).
30556368	3	59	theme	mRNA	677:680	arg1	expression					682:691	the mRNA expression	673:691	the mRNA expression of lipogenic enzymes	673:712	Mammary tissue biopsies were performed to analyze the mRNA expression of lipogenic enzymes.
30556368	0	60	theme	mammary	157:163	arg1	enzymes					175:181	mammary lipogenic enzymes	157:181	mammary lipogenic enzymes	157:181	Effects of isonitrogenous and isocaloric total mixed ration composed of forages with different quality on milk fatty acid composition and gene expression of mammary lipogenic enzymes in mid-lactating dairy cows.
30556368	5	61	theme	milk	810:813	arg1	yield					815:819	The milk yield	806:819	The milk yield	806:819	The milk yield, fat content and saturated fatty acid (SFA) and short- and medium-chain fatty acid (SMFA) contents in milk were lower in the CS diet than in the MF diet, but the unsaturated FA and long-chain FA contents were higher.
30556368	7	62	from	effects	1183:1189	arg1	factors					1220:1226	the nuclear transcription factors	1194:1226	the nuclear transcription factors	1194:1226	No effects on the nuclear transcription factors were observed between the two treatments.
30556368	0	63	from	quality	95:101	arg1	composition					122:132	milk fatty acid composition	106:132	milk fatty acid composition	106:132	Effects of isonitrogenous and isocaloric total mixed ration composed of forages with different quality on milk fatty acid composition and gene expression of mammary lipogenic enzymes in mid-lactating dairy cows.
30556368	0	63	from	quality	95:101	arg1	expression					143:152	gene expression	138:152	gene expression of mammary lipogenic enzymes in mid-lactating dairy cows	138:209	Effects of isonitrogenous and isocaloric total mixed ration composed of forages with different quality on milk fatty acid composition and gene expression of mammary lipogenic enzymes in mid-lactating dairy cows.
30556368	1	64	theme	lipogenic	422:430	arg1	enzymes					432:438	mammary lipogenic enzymes	414:438	mammary lipogenic enzymes in dairy cows	414:452	The aim of this study was to determine the effects of isonitrogenous and isocaloric diets containing different qualities of forages and concentrate content on milk fat composition and genes that encode mammary lipogenic enzymes in dairy cows.
30556368	1	64	theme	lipogenic	422:430	arg1	genes					396:400	genes	396:400	genes that encode mammary lipogenic enzymes in dairy cows	396:452	The aim of this study was to determine the effects of isonitrogenous and isocaloric diets containing different qualities of forages and concentrate content on milk fat composition and genes that encode mammary lipogenic enzymes in dairy cows.
30556368	2	65	theme	cows	478:481	arg1	total					457:461	A total	455:461	A total of 20 Holstein cows	455:481	A total of 20 Holstein cows were assigned to 1 of 2 treatment diets composed of either mixed forages (MF, starch : 21.50%) or corn stover forage (CS, starch : 25.39%).
30556368	3	66	theme	enzymes	706:712	arg1	expression					682:691	the mRNA expression	673:691	the mRNA expression of lipogenic enzymes	673:712	Mammary tissue biopsies were performed to analyze the mRNA expression of lipogenic enzymes.
30556368	6	67	theme	MF	1166:1167	arg1	treatment					1169:1177	the MF treatment	1162:1177	the MF treatment	1162:1177	Genes involved in de novo FA synthesis, FA uptake and transport, and Δ9-desaturation were lower in the CS treatment than in the MF treatment.
30556368	8	68	theme	stover	1299:1304	arg1	diet					1306:1309	corn stover diet	1294:1309	corn stover diet	1294:1309	The data indicated that corn stover diet reduced the milk yield, fat content, SMFA, and SFA contents in milk, as well as the gene expression of mammary lipogenic enzymes in dairy cows.
30556368	5	69	theme	MF	966:967	arg1	diet					969:972	the MF diet	962:972	the MF diet	962:972	The milk yield, fat content and saturated fatty acid (SFA) and short- and medium-chain fatty acid (SMFA) contents in milk were lower in the CS diet than in the MF diet, but the unsaturated FA and long-chain FA contents were higher.
30556368	5	70	theme	saturated	838:846	arg1	SFA					860:862	SFA	860:862	SFA	860:862	The milk yield, fat content and saturated fatty acid (SFA) and short- and medium-chain fatty acid (SMFA) contents in milk were lower in the CS diet than in the MF diet, but the unsaturated FA and long-chain FA contents were higher.
30556368	5	70	theme	saturated	838:846	arg1	acid					854:857	saturated fatty acid	838:857	saturated fatty acid (SFA)	838:863	The milk yield, fat content and saturated fatty acid (SFA) and short- and medium-chain fatty acid (SMFA) contents in milk were lower in the CS diet than in the MF diet, but the unsaturated FA and long-chain FA contents were higher.
30556368	5	70	theme	saturated	838:846	arg1	SMFA					905:908	SMFA	905:908	SMFA	905:908	The milk yield, fat content and saturated fatty acid (SFA) and short- and medium-chain fatty acid (SMFA) contents in milk were lower in the CS diet than in the MF diet, but the unsaturated FA and long-chain FA contents were higher.
30556368	2	71	dep	stover	586:591	arg1	CS					601:602	CS	601:602	CS	601:602	A total of 20 Holstein cows were assigned to 1 of 2 treatment diets composed of either mixed forages (MF, starch : 21.50%) or corn stover forage (CS, starch : 25.39%).
30556368	2	71	dep	stover	586:591	arg1	forage					593:598	forage	593:598	forage	593:598	A total of 20 Holstein cows were assigned to 1 of 2 treatment diets composed of either mixed forages (MF, starch : 21.50%) or corn stover forage (CS, starch : 25.39%).
30556368	2	71	dep	stover	586:591	arg1	starch					605:610	starch	605:610	starch	605:610	A total of 20 Holstein cows were assigned to 1 of 2 treatment diets composed of either mixed forages (MF, starch : 21.50%) or corn stover forage (CS, starch : 25.39%).
30556368	2	72	theme	corn	581:584	arg1	stover					586:591	corn stover forage	581:598	corn stover forage (CS, starch : 25.39%)	581:620	A total of 20 Holstein cows were assigned to 1 of 2 treatment diets composed of either mixed forages (MF, starch : 21.50%) or corn stover forage (CS, starch : 25.39%).
30556368	8	73	from	expression	1400:1409	arg1	milk					1374:1377	milk	1374:1377	milk	1374:1377	The data indicated that corn stover diet reduced the milk yield, fat content, SMFA, and SFA contents in milk, as well as the gene expression of mammary lipogenic enzymes in dairy cows.
30556368	8	73	from	expression	1400:1409	arg1	cows					1449:1452	dairy cows	1443:1452	dairy cows	1443:1452	The data indicated that corn stover diet reduced the milk yield, fat content, SMFA, and SFA contents in milk, as well as the gene expression of mammary lipogenic enzymes in dairy cows.
30556368	0	74	theme	milk	106:109	arg1	composition					122:132	milk fatty acid composition	106:132	milk fatty acid composition	106:132	Effects of isonitrogenous and isocaloric total mixed ration composed of forages with different quality on milk fatty acid composition and gene expression of mammary lipogenic enzymes in mid-lactating dairy cows.
30556368	1	75	theme	content	360:366	arg1	qualities					323:331	different qualities	313:331	different qualities of forages and concentrate content	313:366	The aim of this study was to determine the effects of isonitrogenous and isocaloric diets containing different qualities of forages and concentrate content on milk fat composition and genes that encode mammary lipogenic enzymes in dairy cows.
30556368	5	76	theme	acid	854:857	arg1	contents					911:918	The milk yield, fat content and saturated fatty acid (SFA) and short- and medium-chain fatty acid (SMFA) contents	806:918	The milk yield, fat content and saturated fatty acid (SFA) and short- and medium-chain fatty acid (SMFA) contents in milk	806:926	The milk yield, fat content and saturated fatty acid (SFA) and short- and medium-chain fatty acid (SMFA) contents in milk were lower in the CS diet than in the MF diet, but the unsaturated FA and long-chain FA contents were higher.
30556368	8	77	theme	milk	1323:1326	arg1	yield					1328:1332	milk yield	1323:1332	milk yield	1323:1332	The data indicated that corn stover diet reduced the milk yield, fat content, SMFA, and SFA contents in milk, as well as the gene expression of mammary lipogenic enzymes in dairy cows.
30556368	0	78	from	composition	122:132	arg1	cows					206:209	mid-lactating dairy cows	186:209	mid-lactating dairy cows	186:209	Effects of isonitrogenous and isocaloric total mixed ration composed of forages with different quality on milk fatty acid composition and gene expression of mammary lipogenic enzymes in mid-lactating dairy cows.
30556368	1	79	theme	milk	371:374	arg1	composition					380:390	milk fat composition	371:390	milk fat composition	371:390	The aim of this study was to determine the effects of isonitrogenous and isocaloric diets containing different qualities of forages and concentrate content on milk fat composition and genes that encode mammary lipogenic enzymes in dairy cows.
30556368	5	80	theme	content	826:832	arg1	contents					911:918	The milk yield, fat content and saturated fatty acid (SFA) and short- and medium-chain fatty acid (SMFA) contents	806:918	The milk yield, fat content and saturated fatty acid (SFA) and short- and medium-chain fatty acid (SMFA) contents in milk	806:926	The milk yield, fat content and saturated fatty acid (SFA) and short- and medium-chain fatty acid (SMFA) contents in milk were lower in the CS diet than in the MF diet, but the unsaturated FA and long-chain FA contents were higher.
30556368	8	81	theme	content	1339:1345	arg1	contents					1362:1369	the milk yield, fat content, SMFA, and SFA contents	1319:1369	the milk yield, fat content, SMFA, and SFA contents in milk	1319:1377	The data indicated that corn stover diet reduced the milk yield, fat content, SMFA, and SFA contents in milk, as well as the gene expression of mammary lipogenic enzymes in dairy cows.
30556368	0	82	theme	total	41:45	arg1	ration					53:58	isonitrogenous and isocaloric total mixed ration	11:58	isonitrogenous and isocaloric total mixed ration	11:58	Effects of isonitrogenous and isocaloric total mixed ration composed of forages with different quality on milk fatty acid composition and gene expression of mammary lipogenic enzymes in mid-lactating dairy cows.
30556368	5	83	theme	long-chain	1002:1011	arg1	contents					1016:1023	the unsaturated FA and long-chain FA contents	979:1023	contents	1016:1023	The milk yield, fat content and saturated fatty acid (SFA) and short- and medium-chain fatty acid (SMFA) contents in milk were lower in the CS diet than in the MF diet, but the unsaturated FA and long-chain FA contents were higher.
30556368	8	84	theme	SMFA	1348:1351	arg1	contents					1362:1369	the milk yield, fat content, SMFA, and SFA contents	1319:1369	the milk yield, fat content, SMFA, and SFA contents in milk	1319:1377	The data indicated that corn stover diet reduced the milk yield, fat content, SMFA, and SFA contents in milk, as well as the gene expression of mammary lipogenic enzymes in dairy cows.
30556368	4	85	theme	matter	719:724	arg1	intake					726:731	Dry matter intake	715:731	Dry matter intake	715:731	Dry matter intake, body weight, milk protein, and lactose were not affected by treatments.
30556368	0	86	theme	ration	53:58	arg1	Effects					0:6	Effects	0:6	Effects of isonitrogenous and isocaloric total mixed ration	0:58	Effects of isonitrogenous and isocaloric total mixed ration composed of forages with different quality on milk fatty acid composition and gene expression of mammary lipogenic enzymes in mid-lactating dairy cows.
30556368	1	87	theme	diets	296:300	arg1	effects					255:261	the effects	251:261	the effects of isonitrogenous and isocaloric diets containing different qualities of forages and concentrate content on milk fat composition and genes that encode mammary lipogenic enzymes in dairy cows	251:452	The aim of this study was to determine the effects of isonitrogenous and isocaloric diets containing different qualities of forages and concentrate content on milk fat composition and genes that encode mammary lipogenic enzymes in dairy cows.
30556368	5	88	theme	fatty	893:897	arg1	acid					899:902	short- and medium-chain fatty acid	869:902	short- and medium-chain fatty acid	869:902	The milk yield, fat content and saturated fatty acid (SFA) and short- and medium-chain fatty acid (SMFA) contents in milk were lower in the CS diet than in the MF diet, but the unsaturated FA and long-chain FA contents were higher.
30556368	8	89	theme	gene	1395:1398	arg1	expression					1400:1409	the gene expression	1391:1409	the gene expression of mammary lipogenic enzymes in dairy cows	1391:1452	The data indicated that corn stover diet reduced the milk yield, fat content, SMFA, and SFA contents in milk, as well as the gene expression of mammary lipogenic enzymes in dairy cows.
30556368	1	90	theme	different	313:321	arg1	qualities					323:331	different qualities	313:331	different qualities of forages and concentrate content	313:366	The aim of this study was to determine the effects of isonitrogenous and isocaloric diets containing different qualities of forages and concentrate content on milk fat composition and genes that encode mammary lipogenic enzymes in dairy cows.
30556368	8	91	theme	lipogenic	1422:1430	arg1	enzymes					1432:1438	mammary lipogenic enzymes	1414:1438	mammary lipogenic enzymes	1414:1438	The data indicated that corn stover diet reduced the milk yield, fat content, SMFA, and SFA contents in milk, as well as the gene expression of mammary lipogenic enzymes in dairy cows.
30556368	1	92	theme	study	228:232	arg1	aim					216:218	The aim	212:218	The aim of this study	212:232	The aim of this study was to determine the effects of isonitrogenous and isocaloric diets containing different qualities of forages and concentrate content on milk fat composition and genes that encode mammary lipogenic enzymes in dairy cows.
29951005	0	0	theme	N-Acetylglucosamine	109:127	arg1	Transferase					129:139	O-linked N-Acetylglucosamine Transferase	100:139	O-linked N-Acetylglucosamine Transferase Expression	100:150	Corrigendum: MicroRNA-200a/200b Modulate High Glucose-Induced Endothelial Inflammation by Targeting O-linked N-Acetylglucosamine Transferase Expression.
29951005	0	1	dep	Corrigendum	0:10	arg1	MicroRNA-200a/200b					13:30	MicroRNA-200a/200b	13:30	Corrigendum: MicroRNA-200a/200b	0:30	Corrigendum: MicroRNA-200a/200b Modulate High Glucose-Induced Endothelial Inflammation by Targeting O-linked N-Acetylglucosamine Transferase Expression.
29951005	1	2	from	article	172:178	arg1	p.					183:184	p.	183:184	p.	183:184	[This corrects the article on p. 355 in vol.
29951005	1	2	from	article	172:178	arg1	vol					193:195	vol	193:195	vol	193:195	[This corrects the article on p. 355 in vol.
29951005	0	3	theme	Glucose-Induced	46:60	arg1	Inflammation					74:85	High Glucose-Induced Endothelial Inflammation	41:85	High Glucose-Induced Endothelial Inflammation	41:85	Corrigendum: MicroRNA-200a/200b Modulate High Glucose-Induced Endothelial Inflammation by Targeting O-linked N-Acetylglucosamine Transferase Expression.
29951005	0	4	theme	O-linked	100:107	arg1	Transferase					129:139	O-linked N-Acetylglucosamine Transferase	100:139	O-linked N-Acetylglucosamine Transferase Expression	100:150	Corrigendum: MicroRNA-200a/200b Modulate High Glucose-Induced Endothelial Inflammation by Targeting O-linked N-Acetylglucosamine Transferase Expression.
29951005	0	5	theme	High	41:44	arg1	Inflammation					74:85	High Glucose-Induced Endothelial Inflammation	41:85	High Glucose-Induced Endothelial Inflammation	41:85	Corrigendum: MicroRNA-200a/200b Modulate High Glucose-Induced Endothelial Inflammation by Targeting O-linked N-Acetylglucosamine Transferase Expression.
29951005	0	6	theme	Transferase	129:139	arg1	Expression					141:150	O-linked N-Acetylglucosamine Transferase Expression	100:150	O-linked N-Acetylglucosamine Transferase Expression	100:150	Corrigendum: MicroRNA-200a/200b Modulate High Glucose-Induced Endothelial Inflammation by Targeting O-linked N-Acetylglucosamine Transferase Expression.
29951005	0	7	link	O-linked	100:107	arg1	Transferase					129:139	O-linked N-Acetylglucosamine Transferase	100:139	O-linked N-Acetylglucosamine Transferase Expression	100:150	Corrigendum: MicroRNA-200a/200b Modulate High Glucose-Induced Endothelial Inflammation by Targeting O-linked N-Acetylglucosamine Transferase Expression.
29951005	2	8	dep	9	198:198	arg1	29720943					207:214	29720943	207:214	29720943	207:214	9, PMID: 29720943.]
29951005	0	9	theme	Endothelial	62:72	arg1	Inflammation					74:85	High Glucose-Induced Endothelial Inflammation	41:85	High Glucose-Induced Endothelial Inflammation	41:85	Corrigendum: MicroRNA-200a/200b Modulate High Glucose-Induced Endothelial Inflammation by Targeting O-linked N-Acetylglucosamine Transferase Expression.
31211803	11	0	theme	decreased	1422:1430	arg1	expressions					1455:1465	decreased tight junction protein expressions	1422:1465	decreased tight junction protein expressions	1422:1465	In addition, the intestinal tight junction barrier was disrupted by antibiotic treatment, as evidenced by increased intestinal paracellular permeability to FITC-dextran, decreased tight junction protein expressions, and disrupted ZO-1 morphology.
31211803	5	1	theme	female	590:595	arg1	mice					605:608	Healthy adult female C57BL/6 mice	576:608	Healthy adult female C57BL/6 mice	576:608	Healthy adult female C57BL/6 mice were treated with a broad-spectrum antibiotic cocktail for 14 days.
31211803	4	2	from	influence	474:482	arg1	barrier					528:534	intestinal tight junction barrier	502:534	intestinal tight junction barrier	502:534	Therefore, this study was aimed to investigate the influence of antibiotics on intestinal tight junction barrier and the possible underlying mechanisms.
31211803	4	2	from	influence	474:482	arg1	mechanisms					564:573	the possible underlying mechanisms	540:573	the possible underlying mechanisms	540:573	Therefore, this study was aimed to investigate the influence of antibiotics on intestinal tight junction barrier and the possible underlying mechanisms.
31211803	0	3	theme	microbiota	85:94	arg1	dysbiosis					96:104	microbiota dysbiosis	85:104	microbiota dysbiosis	85:104	Antibiotics induced intestinal tight junction barrier dysfunction is associated with microbiota dysbiosis, activated NLRP3 inflammasome and autophagy.
31211803	10	4	theme	healthy	1194:1200	arg1	mice					1202:1205	healthy mice	1194:1205	healthy mice	1194:1205	Antibiotics remarkably altered intestinal microbiota composition in healthy mice, accompanying reduced SCFAs' concentrations.
31211803	11	5	theme	tight	1432:1436	arg1	expressions					1455:1465	decreased tight junction protein expressions	1422:1465	decreased tight junction protein expressions	1422:1465	In addition, the intestinal tight junction barrier was disrupted by antibiotic treatment, as evidenced by increased intestinal paracellular permeability to FITC-dextran, decreased tight junction protein expressions, and disrupted ZO-1 morphology.
31211803	7	6	theme	intestinal	887:896	arg1	permeability					911:922	In vivo intestinal paracellular permeability	879:922	In vivo intestinal paracellular permeability to fluorescein isothiocyanate-dextran (FITC-dextran)	879:975	In vivo intestinal paracellular permeability to fluorescein isothiocyanate-dextran (FITC-dextran) was measured.
31211803	6	7	theme	16S	678:680	arg1	sequencing					696:705	16S rDNA Illumina sequencing	678:705	16S rDNA Illumina sequencing	678:705	16S rDNA Illumina sequencing and headspace gas chromatography-mass spectrometry (HS-GC/MS) were respectively used to analyze microbial community and to detect short-chain fatty acids (SCFAs) contents.
31211803	0	8	theme	activated	107:115	arg1	inflammasome					123:134	activated NLRP3 inflammasome	107:134	activated NLRP3 inflammasome	107:134	Antibiotics induced intestinal tight junction barrier dysfunction is associated with microbiota dysbiosis, activated NLRP3 inflammasome and autophagy.
31211803	4	9	theme	underlying	553:562	arg1	mechanisms					564:573	the possible underlying mechanisms	540:573	the possible underlying mechanisms	540:573	Therefore, this study was aimed to investigate the influence of antibiotics on intestinal tight junction barrier and the possible underlying mechanisms.
31211803	11	10	theme	tight	1280:1284	arg1	barrier					1295:1301	the intestinal tight junction barrier	1265:1301	the intestinal tight junction barrier	1265:1301	In addition, the intestinal tight junction barrier was disrupted by antibiotic treatment, as evidenced by increased intestinal paracellular permeability to FITC-dextran, decreased tight junction protein expressions, and disrupted ZO-1 morphology.
31211803	11	11	theme	antibiotic	1320:1329	arg1	treatment					1331:1339	antibiotic treatment	1320:1339	antibiotic treatment	1320:1339	In addition, the intestinal tight junction barrier was disrupted by antibiotic treatment, as evidenced by increased intestinal paracellular permeability to FITC-dextran, decreased tight junction protein expressions, and disrupted ZO-1 morphology.
31211803	3	12	theme	gastrointestinal	396:411	arg1	diseases					413:420	some gastrointestinal diseases	391:420	some gastrointestinal diseases	391:420	Intestinal microbiota dysbiosis is involved in the occurrence of some gastrointestinal diseases.
31211803	4	13	theme	junction	519:526	arg1	barrier					528:534	intestinal tight junction barrier	502:534	intestinal tight junction barrier	502:534	Therefore, this study was aimed to investigate the influence of antibiotics on intestinal tight junction barrier and the possible underlying mechanisms.
31211803	2	14	theme	intestinal	303:312	arg1	microbiota					314:323	intestinal microbiota	303:323	intestinal microbiota	303:323	Applying antibiotics to treat infections is common in clinical practice, which may affect intestinal microbiota.
31211803	13	15	from	dysbiosis	1721:1729	arg1	mice					1778:1781	mice	1778:1781	mice	1778:1781	In conclusion, intestinal epithelial tight junction barrier dysfunction induced by antibiotics is associated with intestinal microbiota dysbiosis, activated NLRP3 inflammasome and autophagy in mice.
31211803	7	16	theme	fluorescein	927:937	arg1	FITC-dextran					963:974	FITC-dextran	963:974	FITC-dextran	963:974	In vivo intestinal paracellular permeability to fluorescein isothiocyanate-dextran (FITC-dextran) was measured.
31211803	7	16	theme	fluorescein	927:937	arg1	isothiocyanate-dextran					939:960	fluorescein isothiocyanate-dextran	927:960	fluorescein isothiocyanate-dextran (FITC-dextran)	927:975	In vivo intestinal paracellular permeability to fluorescein isothiocyanate-dextran (FITC-dextran) was measured.
31211803	4	17	theme	tight	513:517	arg1	barrier					528:534	intestinal tight junction barrier	502:534	intestinal tight junction barrier	502:534	Therefore, this study was aimed to investigate the influence of antibiotics on intestinal tight junction barrier and the possible underlying mechanisms.
31211803	6	18	theme	rDNA	682:685	arg1	sequencing					696:705	16S rDNA Illumina sequencing	678:705	16S rDNA Illumina sequencing	678:705	16S rDNA Illumina sequencing and headspace gas chromatography-mass spectrometry (HS-GC/MS) were respectively used to analyze microbial community and to detect short-chain fatty acids (SCFAs) contents.
31211803	11	19	theme	junction	1286:1293	arg1	barrier					1295:1301	the intestinal tight junction barrier	1265:1301	the intestinal tight junction barrier	1265:1301	In addition, the intestinal tight junction barrier was disrupted by antibiotic treatment, as evidenced by increased intestinal paracellular permeability to FITC-dextran, decreased tight junction protein expressions, and disrupted ZO-1 morphology.
31211803	0	20	theme	NLRP3	117:121	arg1	inflammasome					123:134	activated NLRP3 inflammasome	107:134	activated NLRP3 inflammasome	107:134	Antibiotics induced intestinal tight junction barrier dysfunction is associated with microbiota dysbiosis, activated NLRP3 inflammasome and autophagy.
31211803	10	21	theme	reduced	1221:1227	arg1	concentrations					1236:1249	reduced SCFAs' concentrations	1221:1249	reduced SCFAs' concentrations	1221:1249	Antibiotics remarkably altered intestinal microbiota composition in healthy mice, accompanying reduced SCFAs' concentrations.
31211803	12	22	theme	antibiotic	1563:1572	arg1	treatment					1574:1582	antibiotic treatment	1563:1582	antibiotic treatment	1563:1582	Furthermore, NLRP3 inflammasome and autophagy were activated by antibiotic treatment.
31211803	1	23	theme	intestinal	189:198	arg1	homeostasis					200:210	intestinal homeostasis	189:210	intestinal homeostasis	189:210	Tight junction barrier is critical to intestinal homeostasis.
31211803	11	24	theme	ZO-1	1482:1485	arg1	morphology					1487:1496	disrupted ZO-1 morphology	1472:1496	disrupted ZO-1 morphology	1472:1496	In addition, the intestinal tight junction barrier was disrupted by antibiotic treatment, as evidenced by increased intestinal paracellular permeability to FITC-dextran, decreased tight junction protein expressions, and disrupted ZO-1 morphology.
31211803	11	25	theme	junction	1438:1445	arg1	expressions					1455:1465	decreased tight junction protein expressions	1422:1465	decreased tight junction protein expressions	1422:1465	In addition, the intestinal tight junction barrier was disrupted by antibiotic treatment, as evidenced by increased intestinal paracellular permeability to FITC-dextran, decreased tight junction protein expressions, and disrupted ZO-1 morphology.
31211803	9	26	theme	ASC	1121:1123	arg1	distributions					1089:1101	the distributions	1085:1101	the distributions of ZO-1, LC3B and ASC	1085:1123	Immunofluoresence was applied to observe the distributions of ZO-1, LC3B and ASC.
31211803	2	27	from	common	257:262	arg1	practice					276:283	clinical practice	267:283	clinical practice	267:283	Applying antibiotics to treat infections is common in clinical practice, which may affect intestinal microbiota.
31211803	11	28	theme	protein	1447:1453	arg1	expressions					1455:1465	decreased tight junction protein expressions	1422:1465	decreased tight junction protein expressions	1422:1465	In addition, the intestinal tight junction barrier was disrupted by antibiotic treatment, as evidenced by increased intestinal paracellular permeability to FITC-dextran, decreased tight junction protein expressions, and disrupted ZO-1 morphology.
31211803	5	29	theme	broad-spectrum	630:643	arg1	cocktail					656:663	a broad-spectrum antibiotic cocktail	628:663	a broad-spectrum antibiotic cocktail	628:663	Healthy adult female C57BL/6 mice were treated with a broad-spectrum antibiotic cocktail for 14 days.
31211803	13	30	theme	intestinal	1699:1708	arg1	dysbiosis					1721:1729	intestinal microbiota dysbiosis	1699:1729	intestinal microbiota dysbiosis	1699:1729	In conclusion, intestinal epithelial tight junction barrier dysfunction induced by antibiotics is associated with intestinal microbiota dysbiosis, activated NLRP3 inflammasome and autophagy in mice.
31211803	2	31	from	practice	276:283	arg1	common					257:262	common	257:262	common	257:262	Applying antibiotics to treat infections is common in clinical practice, which may affect intestinal microbiota.
31211803	3	32	theme	diseases	413:420	arg1	occurrence					377:386	the occurrence	373:386	the occurrence of some gastrointestinal diseases	373:420	Intestinal microbiota dysbiosis is involved in the occurrence of some gastrointestinal diseases.
31211803	6	33	theme	acids	855:859	arg1	contents					869:876	short-chain fatty acids (SCFAs) contents	837:876	short-chain fatty acids (SCFAs) contents	837:876	16S rDNA Illumina sequencing and headspace gas chromatography-mass spectrometry (HS-GC/MS) were respectively used to analyze microbial community and to detect short-chain fatty acids (SCFAs) contents.
31211803	5	34	theme	antibiotic	645:654	arg1	cocktail					656:663	a broad-spectrum antibiotic cocktail	628:663	a broad-spectrum antibiotic cocktail	628:663	Healthy adult female C57BL/6 mice were treated with a broad-spectrum antibiotic cocktail for 14 days.
31211803	12	35	dep	NLRP3	1512:1516	arg1	inflammasome					1518:1529	inflammasome	1518:1529	inflammasome	1518:1529	Furthermore, NLRP3 inflammasome and autophagy were activated by antibiotic treatment.
31211803	0	36	theme	intestinal	20:29	arg1	barrier					46:52	intestinal tight junction barrier	20:52	intestinal tight junction barrier dysfunction is associated with microbiota dysbiosis, activated NLRP3 inflammasome and autophagy	20:148	Antibiotics induced intestinal tight junction barrier dysfunction is associated with microbiota dysbiosis, activated NLRP3 inflammasome and autophagy.
31211803	11	37	theme	increased	1358:1366	arg1	permeability					1392:1403	increased intestinal paracellular permeability	1358:1403	increased intestinal paracellular permeability to FITC-dextran, decreased tight junction protein expressions, and disrupted ZO-1 morphology	1358:1496	In addition, the intestinal tight junction barrier was disrupted by antibiotic treatment, as evidenced by increased intestinal paracellular permeability to FITC-dextran, decreased tight junction protein expressions, and disrupted ZO-1 morphology.
31211803	4	38	theme	antibiotics	487:497	arg1	influence					474:482	the influence	470:482	the influence of antibiotics on intestinal tight junction barrier and the possible underlying mechanisms	470:573	Therefore, this study was aimed to investigate the influence of antibiotics on intestinal tight junction barrier and the possible underlying mechanisms.
31211803	6	39	used	used	787:790	arg2	HS-GC/MS					759:766	HS-GC/MS	759:766	HS-GC/MS	759:766	16S rDNA Illumina sequencing and headspace gas chromatography-mass spectrometry (HS-GC/MS) were respectively used to analyze microbial community and to detect short-chain fatty acids (SCFAs) contents.
31211803	6	39	used	used	787:790	arg2	sequencing					696:705	16S rDNA Illumina sequencing	678:705	16S rDNA Illumina sequencing	678:705	16S rDNA Illumina sequencing and headspace gas chromatography-mass spectrometry (HS-GC/MS) were respectively used to analyze microbial community and to detect short-chain fatty acids (SCFAs) contents.
31211803	6	39	used	used	787:790	arg2	spectrometry					745:756	headspace gas chromatography-mass spectrometry	711:756	headspace gas chromatography-mass spectrometry (HS-GC/MS)	711:767	16S rDNA Illumina sequencing and headspace gas chromatography-mass spectrometry (HS-GC/MS) were respectively used to analyze microbial community and to detect short-chain fatty acids (SCFAs) contents.
31211803	7	40	theme	paracellular	898:909	arg1	permeability					911:922	In vivo intestinal paracellular permeability	879:922	In vivo intestinal paracellular permeability to fluorescein isothiocyanate-dextran (FITC-dextran)	879:975	In vivo intestinal paracellular permeability to fluorescein isothiocyanate-dextran (FITC-dextran) was measured.
31211803	9	41	theme	ZO-1	1106:1109	arg1	distributions					1089:1101	the distributions	1085:1101	the distributions of ZO-1, LC3B and ASC	1085:1123	Immunofluoresence was applied to observe the distributions of ZO-1, LC3B and ASC.
31211803	13	42	theme	activated	1732:1740	arg1	inflammasome					1748:1759	activated NLRP3 inflammasome	1732:1759	activated NLRP3 inflammasome	1732:1759	In conclusion, intestinal epithelial tight junction barrier dysfunction induced by antibiotics is associated with intestinal microbiota dysbiosis, activated NLRP3 inflammasome and autophagy in mice.
31211803	6	43	theme	chromatography-mass	725:743	arg1	HS-GC/MS					759:766	HS-GC/MS	759:766	HS-GC/MS	759:766	16S rDNA Illumina sequencing and headspace gas chromatography-mass spectrometry (HS-GC/MS) were respectively used to analyze microbial community and to detect short-chain fatty acids (SCFAs) contents.
31211803	6	43	theme	chromatography-mass	725:743	arg1	spectrometry					745:756	headspace gas chromatography-mass spectrometry	711:756	headspace gas chromatography-mass spectrometry (HS-GC/MS)	711:767	16S rDNA Illumina sequencing and headspace gas chromatography-mass spectrometry (HS-GC/MS) were respectively used to analyze microbial community and to detect short-chain fatty acids (SCFAs) contents.
31211803	0	44	theme	junction	37:44	arg1	barrier					46:52	intestinal tight junction barrier	20:52	intestinal tight junction barrier dysfunction is associated with microbiota dysbiosis, activated NLRP3 inflammasome and autophagy	20:148	Antibiotics induced intestinal tight junction barrier dysfunction is associated with microbiota dysbiosis, activated NLRP3 inflammasome and autophagy.
31211803	13	45	theme	microbiota	1710:1719	arg1	dysbiosis					1721:1729	intestinal microbiota dysbiosis	1699:1729	intestinal microbiota dysbiosis	1699:1729	In conclusion, intestinal epithelial tight junction barrier dysfunction induced by antibiotics is associated with intestinal microbiota dysbiosis, activated NLRP3 inflammasome and autophagy in mice.
31211803	4	46	theme	intestinal	502:511	arg1	barrier					528:534	intestinal tight junction barrier	502:534	intestinal tight junction barrier	502:534	Therefore, this study was aimed to investigate the influence of antibiotics on intestinal tight junction barrier and the possible underlying mechanisms.
31211803	0	47	theme	tight	31:35	arg1	barrier					46:52	intestinal tight junction barrier	20:52	intestinal tight junction barrier dysfunction is associated with microbiota dysbiosis, activated NLRP3 inflammasome and autophagy	20:148	Antibiotics induced intestinal tight junction barrier dysfunction is associated with microbiota dysbiosis, activated NLRP3 inflammasome and autophagy.
31211803	5	48	theme	C57BL/6	597:603	arg1	mice					605:608	Healthy adult female C57BL/6 mice	576:608	Healthy adult female C57BL/6 mice	576:608	Healthy adult female C57BL/6 mice were treated with a broad-spectrum antibiotic cocktail for 14 days.
31211803	6	49	theme	microbial	803:811	arg1	community					813:821	microbial community	803:821	microbial community	803:821	16S rDNA Illumina sequencing and headspace gas chromatography-mass spectrometry (HS-GC/MS) were respectively used to analyze microbial community and to detect short-chain fatty acids (SCFAs) contents.
31211803	9	50	theme	LC3B	1112:1115	arg1	distributions					1089:1101	the distributions	1085:1101	the distributions of ZO-1, LC3B and ASC	1085:1123	Immunofluoresence was applied to observe the distributions of ZO-1, LC3B and ASC.
31211803	3	51	theme	microbiota	337:346	arg1	dysbiosis					348:356	Intestinal microbiota dysbiosis	326:356	Intestinal microbiota dysbiosis	326:356	Intestinal microbiota dysbiosis is involved in the occurrence of some gastrointestinal diseases.
31211803	7	52	theme	In	879:880	arg1	permeability					911:922	In vivo intestinal paracellular permeability	879:922	In vivo intestinal paracellular permeability to fluorescein isothiocyanate-dextran (FITC-dextran)	879:975	In vivo intestinal paracellular permeability to fluorescein isothiocyanate-dextran (FITC-dextran) was measured.
31211803	0	53	dep	barrier	46:52	arg1	associated					69:78	associated	69:78	is associated with microbiota dysbiosis, activated NLRP3 inflammasome and autophagy	66:148	Antibiotics induced intestinal tight junction barrier dysfunction is associated with microbiota dysbiosis, activated NLRP3 inflammasome and autophagy.
31211803	13	54	theme	intestinal	1600:1609	arg1	barrier					1637:1643	intestinal epithelial tight junction barrier	1600:1643	intestinal epithelial tight junction barrier dysfunction induced by antibiotics	1600:1678	In conclusion, intestinal epithelial tight junction barrier dysfunction induced by antibiotics is associated with intestinal microbiota dysbiosis, activated NLRP3 inflammasome and autophagy in mice.
31211803	7	55	dep	In	879:880	arg1	vivo					882:885	vivo	882:885	vivo	882:885	In vivo intestinal paracellular permeability to fluorescein isothiocyanate-dextran (FITC-dextran) was measured.
31211803	13	56	theme	barrier	1637:1643	arg1	dysfunction					1645:1655	intestinal epithelial tight junction barrier dysfunction	1600:1655	intestinal epithelial tight junction barrier dysfunction induced by antibiotics	1600:1678	In conclusion, intestinal epithelial tight junction barrier dysfunction induced by antibiotics is associated with intestinal microbiota dysbiosis, activated NLRP3 inflammasome and autophagy in mice.
31211803	8	57	theme	Protein	991:997	arg1	expression					999:1008	Protein expression	991:1008	Protein expression	991:1008	Protein expression was determined by immunoblotting.
31211803	13	58	theme	epithelial	1611:1620	arg1	barrier					1637:1643	intestinal epithelial tight junction barrier	1600:1643	intestinal epithelial tight junction barrier dysfunction induced by antibiotics	1600:1678	In conclusion, intestinal epithelial tight junction barrier dysfunction induced by antibiotics is associated with intestinal microbiota dysbiosis, activated NLRP3 inflammasome and autophagy in mice.
31211803	6	59	theme	gas	721:723	arg1	HS-GC/MS					759:766	HS-GC/MS	759:766	HS-GC/MS	759:766	16S rDNA Illumina sequencing and headspace gas chromatography-mass spectrometry (HS-GC/MS) were respectively used to analyze microbial community and to detect short-chain fatty acids (SCFAs) contents.
31211803	6	59	theme	gas	721:723	arg1	spectrometry					745:756	headspace gas chromatography-mass spectrometry	711:756	headspace gas chromatography-mass spectrometry (HS-GC/MS)	711:767	16S rDNA Illumina sequencing and headspace gas chromatography-mass spectrometry (HS-GC/MS) were respectively used to analyze microbial community and to detect short-chain fatty acids (SCFAs) contents.
31211803	6	60	theme	fatty	849:853	arg1	SCFAs					862:866	SCFAs	862:866	SCFAs	862:866	16S rDNA Illumina sequencing and headspace gas chromatography-mass spectrometry (HS-GC/MS) were respectively used to analyze microbial community and to detect short-chain fatty acids (SCFAs) contents.
31211803	6	60	theme	fatty	849:853	arg1	acids					855:859	short-chain fatty acids	837:859	short-chain fatty acids (SCFAs) contents	837:876	16S rDNA Illumina sequencing and headspace gas chromatography-mass spectrometry (HS-GC/MS) were respectively used to analyze microbial community and to detect short-chain fatty acids (SCFAs) contents.
31211803	11	61	theme	intestinal	1368:1377	arg1	permeability					1392:1403	increased intestinal paracellular permeability	1358:1403	increased intestinal paracellular permeability to FITC-dextran, decreased tight junction protein expressions, and disrupted ZO-1 morphology	1358:1496	In addition, the intestinal tight junction barrier was disrupted by antibiotic treatment, as evidenced by increased intestinal paracellular permeability to FITC-dextran, decreased tight junction protein expressions, and disrupted ZO-1 morphology.
31211803	10	62	theme	microbiota	1168:1177	arg1	composition					1179:1189	intestinal microbiota composition	1157:1189	intestinal microbiota composition	1157:1189	Antibiotics remarkably altered intestinal microbiota composition in healthy mice, accompanying reduced SCFAs' concentrations.
31211803	11	63	theme	intestinal	1269:1278	arg1	barrier					1295:1301	the intestinal tight junction barrier	1265:1301	the intestinal tight junction barrier	1265:1301	In addition, the intestinal tight junction barrier was disrupted by antibiotic treatment, as evidenced by increased intestinal paracellular permeability to FITC-dextran, decreased tight junction protein expressions, and disrupted ZO-1 morphology.
31211803	13	64	theme	NLRP3	1742:1746	arg1	inflammasome					1748:1759	activated NLRP3 inflammasome	1732:1759	activated NLRP3 inflammasome	1732:1759	In conclusion, intestinal epithelial tight junction barrier dysfunction induced by antibiotics is associated with intestinal microbiota dysbiosis, activated NLRP3 inflammasome and autophagy in mice.
31211803	13	65	theme	tight	1622:1626	arg1	barrier					1637:1643	intestinal epithelial tight junction barrier	1600:1643	intestinal epithelial tight junction barrier dysfunction induced by antibiotics	1600:1678	In conclusion, intestinal epithelial tight junction barrier dysfunction induced by antibiotics is associated with intestinal microbiota dysbiosis, activated NLRP3 inflammasome and autophagy in mice.
31211803	6	66	theme	headspace	711:719	arg1	HS-GC/MS					759:766	HS-GC/MS	759:766	HS-GC/MS	759:766	16S rDNA Illumina sequencing and headspace gas chromatography-mass spectrometry (HS-GC/MS) were respectively used to analyze microbial community and to detect short-chain fatty acids (SCFAs) contents.
31211803	6	66	theme	headspace	711:719	arg1	spectrometry					745:756	headspace gas chromatography-mass spectrometry	711:756	headspace gas chromatography-mass spectrometry (HS-GC/MS)	711:767	16S rDNA Illumina sequencing and headspace gas chromatography-mass spectrometry (HS-GC/MS) were respectively used to analyze microbial community and to detect short-chain fatty acids (SCFAs) contents.
31211803	11	67	theme	disrupted	1472:1480	arg1	morphology					1487:1496	disrupted ZO-1 morphology	1472:1496	disrupted ZO-1 morphology	1472:1496	In addition, the intestinal tight junction barrier was disrupted by antibiotic treatment, as evidenced by increased intestinal paracellular permeability to FITC-dextran, decreased tight junction protein expressions, and disrupted ZO-1 morphology.
31211803	3	68	theme	Intestinal	326:335	arg1	dysbiosis					348:356	Intestinal microbiota dysbiosis	326:356	Intestinal microbiota dysbiosis	326:356	Intestinal microbiota dysbiosis is involved in the occurrence of some gastrointestinal diseases.
31211803	10	69	theme	intestinal	1157:1166	arg1	composition					1179:1189	intestinal microbiota composition	1157:1189	intestinal microbiota composition	1157:1189	Antibiotics remarkably altered intestinal microbiota composition in healthy mice, accompanying reduced SCFAs' concentrations.
31211803	6	70	theme	short-chain	837:847	arg1	SCFAs					862:866	SCFAs	862:866	SCFAs	862:866	16S rDNA Illumina sequencing and headspace gas chromatography-mass spectrometry (HS-GC/MS) were respectively used to analyze microbial community and to detect short-chain fatty acids (SCFAs) contents.
31211803	6	70	theme	short-chain	837:847	arg1	acids					855:859	short-chain fatty acids	837:859	short-chain fatty acids (SCFAs) contents	837:876	16S rDNA Illumina sequencing and headspace gas chromatography-mass spectrometry (HS-GC/MS) were respectively used to analyze microbial community and to detect short-chain fatty acids (SCFAs) contents.
31211803	1	71	theme	Tight	151:155	arg1	barrier					166:172	Tight junction barrier	151:172	Tight junction barrier	151:172	Tight junction barrier is critical to intestinal homeostasis.
31211803	13	72	from	autophagy	1765:1773	arg1	mice					1778:1781	mice	1778:1781	mice	1778:1781	In conclusion, intestinal epithelial tight junction barrier dysfunction induced by antibiotics is associated with intestinal microbiota dysbiosis, activated NLRP3 inflammasome and autophagy in mice.
31211803	5	73	theme	Healthy	576:582	arg1	mice					605:608	Healthy adult female C57BL/6 mice	576:608	Healthy adult female C57BL/6 mice	576:608	Healthy adult female C57BL/6 mice were treated with a broad-spectrum antibiotic cocktail for 14 days.
31211803	1	74	theme	junction	157:164	arg1	barrier					166:172	Tight junction barrier	151:172	Tight junction barrier	151:172	Tight junction barrier is critical to intestinal homeostasis.
31211803	13	75	theme	junction	1628:1635	arg1	barrier					1637:1643	intestinal epithelial tight junction barrier	1600:1643	intestinal epithelial tight junction barrier dysfunction induced by antibiotics	1600:1678	In conclusion, intestinal epithelial tight junction barrier dysfunction induced by antibiotics is associated with intestinal microbiota dysbiosis, activated NLRP3 inflammasome and autophagy in mice.
31211803	13	76	from	inflammasome	1748:1759	arg1	mice					1778:1781	mice	1778:1781	mice	1778:1781	In conclusion, intestinal epithelial tight junction barrier dysfunction induced by antibiotics is associated with intestinal microbiota dysbiosis, activated NLRP3 inflammasome and autophagy in mice.
31211803	4	77	theme	possible	544:551	arg1	mechanisms					564:573	the possible underlying mechanisms	540:573	the possible underlying mechanisms	540:573	Therefore, this study was aimed to investigate the influence of antibiotics on intestinal tight junction barrier and the possible underlying mechanisms.
31211803	2	78	theme	clinical	267:274	arg1	practice					276:283	clinical practice	267:283	clinical practice	267:283	Applying antibiotics to treat infections is common in clinical practice, which may affect intestinal microbiota.
31211803	5	79	theme	adult	584:588	arg1	mice					605:608	Healthy adult female C57BL/6 mice	576:608	Healthy adult female C57BL/6 mice	576:608	Healthy adult female C57BL/6 mice were treated with a broad-spectrum antibiotic cocktail for 14 days.
31211803	6	80	theme	Illumina	687:694	arg1	sequencing					696:705	16S rDNA Illumina sequencing	678:705	16S rDNA Illumina sequencing	678:705	16S rDNA Illumina sequencing and headspace gas chromatography-mass spectrometry (HS-GC/MS) were respectively used to analyze microbial community and to detect short-chain fatty acids (SCFAs) contents.
31211803	11	81	theme	paracellular	1379:1390	arg1	permeability					1392:1403	increased intestinal paracellular permeability	1358:1403	increased intestinal paracellular permeability to FITC-dextran, decreased tight junction protein expressions, and disrupted ZO-1 morphology	1358:1496	In addition, the intestinal tight junction barrier was disrupted by antibiotic treatment, as evidenced by increased intestinal paracellular permeability to FITC-dextran, decreased tight junction protein expressions, and disrupted ZO-1 morphology.
30862039	7	0	theme	OVX	1213:1215	arg1	mice					1217:1220	The OVX mice	1209:1220	The OVX mice given a high-fat diet (HFD)	1209:1248	The OVX mice given a high-fat diet (HFD) were randomly divided into three groups: OVX+SO, OVX+TO, and OVX+LO.
30862039	4	1	theme	polyunsaturated	710:724	arg1	PUFA					739:742	PUFA	739:742	PUFA	739:742	SO, TO, and LO comprise mainly polyunsaturated fatty acids (PUFA), monounsaturated fatty acids (MUFA), and saturated fatty acids (SFA), respectively.
30862039	4	1	theme	polyunsaturated	710:724	arg1	acids					732:736	polyunsaturated fatty acids	710:736	polyunsaturated fatty acids (PUFA)	710:743	SO, TO, and LO comprise mainly polyunsaturated fatty acids (PUFA), monounsaturated fatty acids (MUFA), and saturated fatty acids (SFA), respectively.
30862039	5	2	theme	fatty	916:920	arg1	MUFA					935:938	MUFA	935:938	MUFA	935:938	However, there have been quite limited studies to investigate the effects of different fatty acids (PUFA, MUFA, and SFA) on physiological adaption and metabolic homeostasis in a menopausal population.
30862039	5	2	theme	fatty	916:920	arg1	SFA					945:947	SFA	945:947	SFA	945:947	However, there have been quite limited studies to investigate the effects of different fatty acids (PUFA, MUFA, and SFA) on physiological adaption and metabolic homeostasis in a menopausal population.
30862039	5	2	theme	fatty	916:920	arg1	PUFA					929:932	PUFA	929:932	PUFA	929:932	However, there have been quite limited studies to investigate the effects of different fatty acids (PUFA, MUFA, and SFA) on physiological adaption and metabolic homeostasis in a menopausal population.
30862039	5	2	theme	fatty	916:920	arg1	acids					922:926	different fatty acids	906:926	different fatty acids (PUFA, MUFA, and SFA)	906:948	However, there have been quite limited studies to investigate the effects of different fatty acids (PUFA, MUFA, and SFA) on physiological adaption and metabolic homeostasis in a menopausal population.
30862039	12	3	theme	glucose	2031:2037	arg1	tolerance					2039:2047	significantly increased oral glucose tolerance	2002:2047	significantly increased oral glucose tolerance	2002:2047	Moreover, replacing SO or LO with TO significantly increased oral glucose tolerance.
30862039	3	4	theme	seed	608:611	arg1	oil					613:615	tea seed oil	604:615	tea seed oil (TO)	604:620	In the present study, we investigated the effect of long-term feeding of edible oils (soybean oil (SO), tea seed oil (TO), and lard oil (LO)) on female ovariectomized (OVX) mice.
30862039	3	4	theme	seed	608:611	arg1	oils					580:583	edible oils	573:583	edible oils (soybean oil (SO), tea seed oil (TO), and lard oil (LO))	573:640	In the present study, we investigated the effect of long-term feeding of edible oils (soybean oil (SO), tea seed oil (TO), and lard oil (LO)) on female ovariectomized (OVX) mice.
30862039	5	5	theme	metabolic	980:988	arg1	homeostasis					990:1000	metabolic homeostasis	980:1000	metabolic homeostasis	980:1000	However, there have been quite limited studies to investigate the effects of different fatty acids (PUFA, MUFA, and SFA) on physiological adaption and metabolic homeostasis in a menopausal population.
30862039	11	6	theme	rich	1947:1950	arg1	HFD					1943:1945	an HFD	1940:1945	an HFD rich in SO or LO	1940:1962	Furthermore, mice given an HFD rich in TO exhibited significantly decreased accumulation of liver lipid droplets and adipocyte sizes of UFP and brown adipose tissue (BAT) compared with those given an HFD rich in SO or LO.
30862039	11	7	theme	tissue	1901:1906	arg1	accumulation					1819:1830	significantly decreased accumulation	1795:1830	significantly decreased accumulation of liver lipid droplets	1795:1854	Furthermore, mice given an HFD rich in TO exhibited significantly decreased accumulation of liver lipid droplets and adipocyte sizes of UFP and brown adipose tissue (BAT) compared with those given an HFD rich in SO or LO.
30862039	11	7	theme	tissue	1901:1906	arg1	sizes					1870:1874	adipocyte sizes	1860:1874	adipocyte sizes of UFP and brown adipose tissue (BAT)	1860:1912	Furthermore, mice given an HFD rich in TO exhibited significantly decreased accumulation of liver lipid droplets and adipocyte sizes of UFP and brown adipose tissue (BAT) compared with those given an HFD rich in SO or LO.
30862039	13	8	theme	nitrogen	2165:2172	arg1	levels					2195:2200	ammonia, blood urea nitrogen, and creatine kinase levels	2145:2200	ammonia, blood urea nitrogen, and creatine kinase levels	2145:2200	Additionally, TO improved endurance performance and exhibited antifatigue activity by lowering ammonia, blood urea nitrogen, and creatine kinase levels.
30862039	6	9	theme	7-week-old	1045:1054	arg1	Institute					1063:1071	7-week-old female Institute	1045:1071	7-week-old female Institute of Cancer Research (ICR) mice	1045:1101	In this study, 7-week-old female Institute of Cancer Research (ICR) mice underwent either bilateral laparotomy (sham group, n = 8) or bilateral oophorectomy (OVX groups, n = 24).
30862039	3	10	theme	present	507:513	arg1	study					515:519	the present study	503:519	the present study	503:519	In the present study, we investigated the effect of long-term feeding of edible oils (soybean oil (SO), tea seed oil (TO), and lard oil (LO)) on female ovariectomized (OVX) mice.
30862039	1	11	theme	total	248:252	arg1	mass					258:261	total fat mass	248:261	total fat mass	248:261	Menopause is associated with changes in body composition (a decline in lean body mass and an increase in total fat mass), leading to an increased risk of metabolic syndrome, nonalcoholic fatty liver disease, and heart disease.
30862039	10	12	theme	TF	1694:1695	arg1	tissues					1675:1681	the relative tissues	1662:1681	the relative tissues of UFP and TF	1662:1695	However, no significant difference in body weight or in the relative tissues of UFP and TF was noted among the OVX+SO and OVX+LO groups.
30862039	5	13	from	adaption	967:974	arg1	population					1018:1027	a menopausal population	1005:1027	a menopausal population	1005:1027	However, there have been quite limited studies to investigate the effects of different fatty acids (PUFA, MUFA, and SFA) on physiological adaption and metabolic homeostasis in a menopausal population.
30862039	6	14	theme	OVX	1188:1190	arg1	groups					1192:1197	OVX groups	1188:1197	OVX groups	1188:1197	In this study, 7-week-old female Institute of Cancer Research (ICR) mice underwent either bilateral laparotomy (sham group, n = 8) or bilateral oophorectomy (OVX groups, n = 24).
30862039	10	15	theme	UFP	1686:1688	arg1	tissues					1675:1681	the relative tissues	1662:1681	the relative tissues of UFP and TF	1662:1695	However, no significant difference in body weight or in the relative tissues of UFP and TF was noted among the OVX+SO and OVX+LO groups.
30862039	4	16	theme	fatty	762:766	arg1	MUFA					775:778	MUFA	775:778	MUFA	775:778	SO, TO, and LO comprise mainly polyunsaturated fatty acids (PUFA), monounsaturated fatty acids (MUFA), and saturated fatty acids (SFA), respectively.
30862039	4	16	theme	fatty	762:766	arg1	acids					768:772	monounsaturated fatty acids	746:772	monounsaturated fatty acids (MUFA)	746:779	SO, TO, and LO comprise mainly polyunsaturated fatty acids (PUFA), monounsaturated fatty acids (MUFA), and saturated fatty acids (SFA), respectively.
30862039	0	17	theme	Obese	116:120	arg1	Mice					137:140	High-Fat-Diet-Induced Obese Ovariectomized Mice	94:140	High-Fat-Diet-Induced Obese Ovariectomized Mice	94:140	Tea Seed Oil Prevents Obesity, Reduces Physical Fatigue, and Improves Exercise Performance in High-Fat-Diet-Induced Obese Ovariectomized Mice.
30862039	1	18	theme	disease	342:348	arg1	risk					289:292	an increased risk	276:292	an increased risk of metabolic syndrome, nonalcoholic fatty liver disease, and heart disease	276:367	Menopause is associated with changes in body composition (a decline in lean body mass and an increase in total fat mass), leading to an increased risk of metabolic syndrome, nonalcoholic fatty liver disease, and heart disease.
30862039	11	19	theme	adipose	1893:1899	arg1	tissue					1901:1906	brown adipose tissue	1887:1906	brown adipose tissue (BAT)	1887:1912	Furthermore, mice given an HFD rich in TO exhibited significantly decreased accumulation of liver lipid droplets and adipocyte sizes of UFP and brown adipose tissue (BAT) compared with those given an HFD rich in SO or LO.
30862039	11	19	theme	adipose	1893:1899	arg1	BAT					1909:1911	BAT	1909:1911	BAT	1909:1911	Furthermore, mice given an HFD rich in TO exhibited significantly decreased accumulation of liver lipid droplets and adipocyte sizes of UFP and brown adipose tissue (BAT) compared with those given an HFD rich in SO or LO.
30862039	14	20	theme	exercise	2300:2307	arg1	performance					2309:2319	exercise performance	2300:2319	exercise performance	2300:2319	Thus, tea seed oil (TO) rich in MUFA could prevent obesity, reduce physical fatigue, and improve exercise performance compared with either SO (PUFA)- or LO(SFA)-rich diets in this HFD-induced obese OVX mice model.
30862039	3	21	theme	female	645:650	arg1	mice					673:676	female ovariectomized (OVX) mice	645:676	female ovariectomized (OVX) mice	645:676	In the present study, we investigated the effect of long-term feeding of edible oils (soybean oil (SO), tea seed oil (TO), and lard oil (LO)) on female ovariectomized (OVX) mice.
30862039	1	22	from	increase	236:243	arg1	mass					224:227	lean body mass	214:227	lean body mass	214:227	Menopause is associated with changes in body composition (a decline in lean body mass and an increase in total fat mass), leading to an increased risk of metabolic syndrome, nonalcoholic fatty liver disease, and heart disease.
30862039	1	22	from	increase	236:243	arg1	mass					258:261	total fat mass	248:261	total fat mass	248:261	Menopause is associated with changes in body composition (a decline in lean body mass and an increase in total fat mass), leading to an increased risk of metabolic syndrome, nonalcoholic fatty liver disease, and heart disease.
30862039	14	23	from	diets	2369:2373	arg1	model					2410:2414	this HFD-induced obese OVX mice model	2378:2414	this HFD-induced obese OVX mice model	2378:2414	Thus, tea seed oil (TO) rich in MUFA could prevent obesity, reduce physical fatigue, and improve exercise performance compared with either SO (PUFA)- or LO(SFA)-rich diets in this HFD-induced obese OVX mice model.
30862039	3	24	theme	long-term	552:560	arg1	feeding					562:568	long-term feeding	552:568	long-term feeding of edible oils (soybean oil (SO), tea seed oil (TO), and lard oil (LO))	552:640	In the present study, we investigated the effect of long-term feeding of edible oils (soybean oil (SO), tea seed oil (TO), and lard oil (LO)) on female ovariectomized (OVX) mice.
30862039	1	25	from	decline	203:209	arg1	mass					224:227	lean body mass	214:227	lean body mass	214:227	Menopause is associated with changes in body composition (a decline in lean body mass and an increase in total fat mass), leading to an increased risk of metabolic syndrome, nonalcoholic fatty liver disease, and heart disease.
30862039	1	25	from	decline	203:209	arg1	mass					258:261	total fat mass	248:261	total fat mass	248:261	Menopause is associated with changes in body composition (a decline in lean body mass and an increase in total fat mass), leading to an increased risk of metabolic syndrome, nonalcoholic fatty liver disease, and heart disease.
30862039	3	26	dep	oils	580:583	arg1	oil					594:596	soybean oil	586:596	soybean oil (SO)	586:601	In the present study, we investigated the effect of long-term feeding of edible oils (soybean oil (SO), tea seed oil (TO), and lard oil (LO)) on female ovariectomized (OVX) mice.
30862039	3	26	dep	oils	580:583	arg1	LO					637:638	LO	637:638	LO	637:638	In the present study, we investigated the effect of long-term feeding of edible oils (soybean oil (SO), tea seed oil (TO), and lard oil (LO)) on female ovariectomized (OVX) mice.
30862039	3	26	dep	oils	580:583	arg1	oil					632:634	lard oil	627:634	lard oil (LO)	627:639	In the present study, we investigated the effect of long-term feeding of edible oils (soybean oil (SO), tea seed oil (TO), and lard oil (LO)) on female ovariectomized (OVX) mice.
30862039	3	26	dep	oils	580:583	arg1	oil					613:615	tea seed oil	604:615	tea seed oil (TO)	604:620	In the present study, we investigated the effect of long-term feeding of edible oils (soybean oil (SO), tea seed oil (TO), and lard oil (LO)) on female ovariectomized (OVX) mice.
30862039	3	26	dep	oils	580:583	arg1	oils					580:583	edible oils	573:583	edible oils (soybean oil (SO), tea seed oil (TO), and lard oil (LO))	573:640	In the present study, we investigated the effect of long-term feeding of edible oils (soybean oil (SO), tea seed oil (TO), and lard oil (LO)) on female ovariectomized (OVX) mice.
30862039	1	27	theme	disease	361:367	arg1	risk					289:292	an increased risk	276:292	an increased risk of metabolic syndrome, nonalcoholic fatty liver disease, and heart disease	276:367	Menopause is associated with changes in body composition (a decline in lean body mass and an increase in total fat mass), leading to an increased risk of metabolic syndrome, nonalcoholic fatty liver disease, and heart disease.
30862039	9	28	dep	weight	1424:1429	arg1	the					1415:1417	the	1415:1417	the	1415:1417	Our findings revealed that the body weight and relative tissues of UFP (uterus fatty peripheral) and total fat (TF) were significantly decreased in the OVX+TO group compared with those in the OVX+SO and OVX+LO groups.
30862039	9	28	dep	weight	1424:1429	arg1	peripheral					1473:1482	peripheral	1473:1482	peripheral	1473:1482	Our findings revealed that the body weight and relative tissues of UFP (uterus fatty peripheral) and total fat (TF) were significantly decreased in the OVX+TO group compared with those in the OVX+SO and OVX+LO groups.
30862039	6	29	theme	Cancer	1076:1081	arg1	mice					1098:1101	Cancer Research (ICR) mice	1076:1101	Cancer Research (ICR) mice	1076:1101	In this study, 7-week-old female Institute of Cancer Research (ICR) mice underwent either bilateral laparotomy (sham group, n = 8) or bilateral oophorectomy (OVX groups, n = 24).
30862039	3	30	theme	oils	580:583	arg1	feeding					562:568	long-term feeding	552:568	long-term feeding of edible oils (soybean oil (SO), tea seed oil (TO), and lard oil (LO))	552:640	In the present study, we investigated the effect of long-term feeding of edible oils (soybean oil (SO), tea seed oil (TO), and lard oil (LO)) on female ovariectomized (OVX) mice.
30862039	4	31	theme	fatty	796:800	arg1	SFA					809:811	SFA	809:811	SFA	809:811	SO, TO, and LO comprise mainly polyunsaturated fatty acids (PUFA), monounsaturated fatty acids (MUFA), and saturated fatty acids (SFA), respectively.
30862039	4	31	theme	fatty	796:800	arg1	acids					802:806	saturated fatty acids	786:806	saturated fatty acids (SFA)	786:812	SO, TO, and LO comprise mainly polyunsaturated fatty acids (PUFA), monounsaturated fatty acids (MUFA), and saturated fatty acids (SFA), respectively.
30862039	5	32	theme	acids	922:926	arg1	effects					895:901	the effects	891:901	the effects of different fatty acids (PUFA, MUFA, and SFA) on physiological adaption and metabolic homeostasis in a menopausal population	891:1027	However, there have been quite limited studies to investigate the effects of different fatty acids (PUFA, MUFA, and SFA) on physiological adaption and metabolic homeostasis in a menopausal population.
30862039	1	33	from	changes	172:178	arg1	composition					188:198	body composition	183:198	body composition (a decline in lean body mass and an increase in total fat mass)	183:262	Menopause is associated with changes in body composition (a decline in lean body mass and an increase in total fat mass), leading to an increased risk of metabolic syndrome, nonalcoholic fatty liver disease, and heart disease.
30862039	13	34	theme	kinase	2188:2193	arg1	levels					2195:2200	ammonia, blood urea nitrogen, and creatine kinase levels	2145:2200	ammonia, blood urea nitrogen, and creatine kinase levels	2145:2200	Additionally, TO improved endurance performance and exhibited antifatigue activity by lowering ammonia, blood urea nitrogen, and creatine kinase levels.
30862039	1	35	theme	metabolic	297:305	arg1	syndrome					307:314	metabolic syndrome	297:314	metabolic syndrome	297:314	Menopause is associated with changes in body composition (a decline in lean body mass and an increase in total fat mass), leading to an increased risk of metabolic syndrome, nonalcoholic fatty liver disease, and heart disease.
30862039	13	36	theme	endurance	2076:2084	arg1	performance					2086:2096	endurance performance	2076:2096	endurance performance	2076:2096	Additionally, TO improved endurance performance and exhibited antifatigue activity by lowering ammonia, blood urea nitrogen, and creatine kinase levels.
30862039	10	37	theme	significant	1618:1628	arg1	difference					1630:1639	no significant difference	1615:1639	no significant difference in body weight or in the relative tissues of UFP and TF	1615:1695	However, no significant difference in body weight or in the relative tissues of UFP and TF was noted among the OVX+SO and OVX+LO groups.
30862039	0	38	theme	Exercise	70:77	arg1	Performance					79:89	Exercise Performance	70:89	Exercise Performance in High-Fat-Diet-Induced Obese Ovariectomized Mice	70:140	Tea Seed Oil Prevents Obesity, Reduces Physical Fatigue, and Improves Exercise Performance in High-Fat-Diet-Induced Obese Ovariectomized Mice.
30862039	2	39	theme	menopause-related	461:477	arg1	syndromes					489:497	menopause-related metabolic syndromes	461:497	menopause-related metabolic syndromes	461:497	A healthy diet to control body weight is an effective strategy for preventing and treating menopause-related metabolic syndromes.
30862039	14	40	from	MUFA	2235:2238	arg1	rich					2227:2230	rich	2227:2230	rich	2227:2230	Thus, tea seed oil (TO) rich in MUFA could prevent obesity, reduce physical fatigue, and improve exercise performance compared with either SO (PUFA)- or LO(SFA)-rich diets in this HFD-induced obese OVX mice model.
30862039	6	41	theme	bilateral	1120:1128	arg1	laparotomy					1130:1139	bilateral laparotomy	1120:1139	bilateral laparotomy (sham group, n = 8)	1120:1159	In this study, 7-week-old female Institute of Cancer Research (ICR) mice underwent either bilateral laparotomy (sham group, n = 8) or bilateral oophorectomy (OVX groups, n = 24).
30862039	1	42	theme	fatty	330:334	arg1	disease					342:348	nonalcoholic fatty liver disease	317:348	nonalcoholic fatty liver disease	317:348	Menopause is associated with changes in body composition (a decline in lean body mass and an increase in total fat mass), leading to an increased risk of metabolic syndrome, nonalcoholic fatty liver disease, and heart disease.
30862039	2	43	dep	diet	380:383	arg1	control					388:394	control	388:394	to control body weight	385:406	A healthy diet to control body weight is an effective strategy for preventing and treating menopause-related metabolic syndromes.
30862039	2	44	theme	body	396:399	arg1	weight					401:406	body weight	396:406	body weight	396:406	A healthy diet to control body weight is an effective strategy for preventing and treating menopause-related metabolic syndromes.
30862039	11	45	theme	rich	1774:1777	arg1	HFD					1770:1772	an HFD	1767:1772	an HFD rich in TO exhibited significantly decreased accumulation of liver lipid droplets and adipocyte sizes of UFP and brown adipose tissue (BAT) compared with those given an HFD rich in SO or LO	1767:1962	Furthermore, mice given an HFD rich in TO exhibited significantly decreased accumulation of liver lipid droplets and adipocyte sizes of UFP and brown adipose tissue (BAT) compared with those given an HFD rich in SO or LO.
30862039	14	46	theme	mice	2405:2408	arg1	model					2410:2414	this HFD-induced obese OVX mice model	2378:2414	this HFD-induced obese OVX mice model	2378:2414	Thus, tea seed oil (TO) rich in MUFA could prevent obesity, reduce physical fatigue, and improve exercise performance compared with either SO (PUFA)- or LO(SFA)-rich diets in this HFD-induced obese OVX mice model.
30862039	0	47	theme	Tea	0:2	arg1	Oil					9:11	Tea Seed Oil	0:11	Tea Seed Oil	0:11	Tea Seed Oil Prevents Obesity, Reduces Physical Fatigue, and Improves Exercise Performance in High-Fat-Diet-Induced Obese Ovariectomized Mice.
30862039	1	48	theme	fat	254:256	arg1	mass					258:261	total fat mass	248:261	total fat mass	248:261	Menopause is associated with changes in body composition (a decline in lean body mass and an increase in total fat mass), leading to an increased risk of metabolic syndrome, nonalcoholic fatty liver disease, and heart disease.
30862039	9	49	theme	relative	1435:1442	arg1	tissues					1444:1450	relative tissues	1435:1450	relative tissues	1435:1450	Our findings revealed that the body weight and relative tissues of UFP (uterus fatty peripheral) and total fat (TF) were significantly decreased in the OVX+TO group compared with those in the OVX+SO and OVX+LO groups.
30862039	3	50	from	effect	542:547	arg1	mice					673:676	female ovariectomized (OVX) mice	645:676	female ovariectomized (OVX) mice	645:676	In the present study, we investigated the effect of long-term feeding of edible oils (soybean oil (SO), tea seed oil (TO), and lard oil (LO)) on female ovariectomized (OVX) mice.
30862039	2	51	theme	healthy	372:378	arg1	diet					380:383	A healthy diet	370:383	A healthy diet to control body weight	370:406	A healthy diet to control body weight is an effective strategy for preventing and treating menopause-related metabolic syndromes.
30862039	2	51	theme	healthy	372:378	arg1	strategy					424:431	an effective strategy	411:431	an effective strategy for preventing and treating menopause-related metabolic syndromes	411:497	A healthy diet to control body weight is an effective strategy for preventing and treating menopause-related metabolic syndromes.
30862039	13	52	theme	ammonia	2145:2151	arg1	levels					2195:2200	ammonia, blood urea nitrogen, and creatine kinase levels	2145:2200	ammonia, blood urea nitrogen, and creatine kinase levels	2145:2200	Additionally, TO improved endurance performance and exhibited antifatigue activity by lowering ammonia, blood urea nitrogen, and creatine kinase levels.
30862039	5	53	theme	physiological	953:965	arg1	adaption					967:974	physiological adaption	953:974	physiological adaption	953:974	However, there have been quite limited studies to investigate the effects of different fatty acids (PUFA, MUFA, and SFA) on physiological adaption and metabolic homeostasis in a menopausal population.
30862039	14	54	theme	rich	2227:2230	arg1	oil					2218:2220	tea seed oil	2209:2220	tea seed oil (TO) rich in MUFA	2209:2238	Thus, tea seed oil (TO) rich in MUFA could prevent obesity, reduce physical fatigue, and improve exercise performance compared with either SO (PUFA)- or LO(SFA)-rich diets in this HFD-induced obese OVX mice model.
30862039	2	55	theme	effective	414:422	arg1	strategy					424:431	an effective strategy	411:431	an effective strategy for preventing and treating menopause-related metabolic syndromes	411:497	A healthy diet to control body weight is an effective strategy for preventing and treating menopause-related metabolic syndromes.
30862039	2	55	theme	effective	414:422	arg1	diet					380:383	A healthy diet	370:383	A healthy diet to control body weight	370:406	A healthy diet to control body weight is an effective strategy for preventing and treating menopause-related metabolic syndromes.
30862039	11	56	theme	lipid	1841:1845	arg1	droplets					1847:1854	liver lipid droplets	1835:1854	liver lipid droplets	1835:1854	Furthermore, mice given an HFD rich in TO exhibited significantly decreased accumulation of liver lipid droplets and adipocyte sizes of UFP and brown adipose tissue (BAT) compared with those given an HFD rich in SO or LO.
30862039	14	57	from	rich	2227:2230	arg1	MUFA					2235:2238	MUFA	2235:2238	MUFA	2235:2238	Thus, tea seed oil (TO) rich in MUFA could prevent obesity, reduce physical fatigue, and improve exercise performance compared with either SO (PUFA)- or LO(SFA)-rich diets in this HFD-induced obese OVX mice model.
30862039	9	58	theme	UFP	1455:1457	arg1	fat					1495:1497	total fat	1489:1497	total fat (TF)	1489:1502	Our findings revealed that the body weight and relative tissues of UFP (uterus fatty peripheral) and total fat (TF) were significantly decreased in the OVX+TO group compared with those in the OVX+SO and OVX+LO groups.
30862039	9	58	theme	UFP	1455:1457	arg1	weight					1424:1429	body weight	1419:1429	body weight	1419:1429	Our findings revealed that the body weight and relative tissues of UFP (uterus fatty peripheral) and total fat (TF) were significantly decreased in the OVX+TO group compared with those in the OVX+SO and OVX+LO groups.
30862039	9	58	theme	UFP	1455:1457	arg1	tissues					1444:1450	relative tissues	1435:1450	relative tissues	1435:1450	Our findings revealed that the body weight and relative tissues of UFP (uterus fatty peripheral) and total fat (TF) were significantly decreased in the OVX+TO group compared with those in the OVX+SO and OVX+LO groups.
30862039	9	58	theme	UFP	1455:1457	arg1	TF					1500:1501	TF	1500:1501	TF	1500:1501	Our findings revealed that the body weight and relative tissues of UFP (uterus fatty peripheral) and total fat (TF) were significantly decreased in the OVX+TO group compared with those in the OVX+SO and OVX+LO groups.
30862039	3	59	theme	soybean	586:592	arg1	oil					594:596	soybean oil	586:596	soybean oil (SO)	586:601	In the present study, we investigated the effect of long-term feeding of edible oils (soybean oil (SO), tea seed oil (TO), and lard oil (LO)) on female ovariectomized (OVX) mice.
30862039	3	59	theme	soybean	586:592	arg1	oils					580:583	edible oils	573:583	edible oils (soybean oil (SO), tea seed oil (TO), and lard oil (LO))	573:640	In the present study, we investigated the effect of long-term feeding of edible oils (soybean oil (SO), tea seed oil (TO), and lard oil (LO)) on female ovariectomized (OVX) mice.
30862039	13	60	theme	antifatigue	2112:2122	arg1	activity					2124:2131	antifatigue activity	2112:2131	antifatigue activity	2112:2131	Additionally, TO improved endurance performance and exhibited antifatigue activity by lowering ammonia, blood urea nitrogen, and creatine kinase levels.
30862039	6	61	dep	oophorectomy	1174:1185	arg1	=					1202:1202	=	1202:1202	=	1202:1202	In this study, 7-week-old female Institute of Cancer Research (ICR) mice underwent either bilateral laparotomy (sham group, n = 8) or bilateral oophorectomy (OVX groups, n = 24).
30862039	14	62	theme	HFD-induced	2383:2393	arg1	model					2410:2414	this HFD-induced obese OVX mice model	2378:2414	this HFD-induced obese OVX mice model	2378:2414	Thus, tea seed oil (TO) rich in MUFA could prevent obesity, reduce physical fatigue, and improve exercise performance compared with either SO (PUFA)- or LO(SFA)-rich diets in this HFD-induced obese OVX mice model.
30862039	13	63	theme	blood	2154:2158	arg1	nitrogen					2165:2172	blood urea nitrogen	2154:2172	blood urea nitrogen	2154:2172	Additionally, TO improved endurance performance and exhibited antifatigue activity by lowering ammonia, blood urea nitrogen, and creatine kinase levels.
30862039	3	64	theme	tea	604:606	arg1	oil					613:615	tea seed oil	604:615	tea seed oil (TO)	604:620	In the present study, we investigated the effect of long-term feeding of edible oils (soybean oil (SO), tea seed oil (TO), and lard oil (LO)) on female ovariectomized (OVX) mice.
30862039	3	64	theme	tea	604:606	arg1	oils					580:583	edible oils	573:583	edible oils (soybean oil (SO), tea seed oil (TO), and lard oil (LO))	573:640	In the present study, we investigated the effect of long-term feeding of edible oils (soybean oil (SO), tea seed oil (TO), and lard oil (LO)) on female ovariectomized (OVX) mice.
30862039	8	65	theme	rich	1326:1329	arg1	HFD					1322:1324	An HFD	1319:1324	An HFD rich in SO, TO	1319:1339	An HFD rich in SO, TO, or LO was given to the OVX mice for 12 weeks.
30862039	1	66	theme	lean	214:217	arg1	mass					224:227	lean body mass	214:227	lean body mass	214:227	Menopause is associated with changes in body composition (a decline in lean body mass and an increase in total fat mass), leading to an increased risk of metabolic syndrome, nonalcoholic fatty liver disease, and heart disease.
30862039	10	67	from	difference	1630:1639	arg1	tissues					1675:1681	the relative tissues	1662:1681	the relative tissues of UFP and TF	1662:1695	However, no significant difference in body weight or in the relative tissues of UFP and TF was noted among the OVX+SO and OVX+LO groups.
30862039	10	67	from	difference	1630:1639	arg1	weight					1649:1654	body weight	1644:1654	body weight	1644:1654	However, no significant difference in body weight or in the relative tissues of UFP and TF was noted among the OVX+SO and OVX+LO groups.
30862039	14	68	theme	tea	2209:2211	arg1	oil					2218:2220	tea seed oil	2209:2220	tea seed oil (TO) rich in MUFA	2209:2238	Thus, tea seed oil (TO) rich in MUFA could prevent obesity, reduce physical fatigue, and improve exercise performance compared with either SO (PUFA)- or LO(SFA)-rich diets in this HFD-induced obese OVX mice model.
30862039	3	69	dep	oil	594:596	arg1	SO					599:600	SO	599:600	SO	599:600	In the present study, we investigated the effect of long-term feeding of edible oils (soybean oil (SO), tea seed oil (TO), and lard oil (LO)) on female ovariectomized (OVX) mice.
30862039	9	70	theme	total	1489:1493	arg1	fat					1495:1497	total fat	1489:1497	total fat (TF)	1489:1502	Our findings revealed that the body weight and relative tissues of UFP (uterus fatty peripheral) and total fat (TF) were significantly decreased in the OVX+TO group compared with those in the OVX+SO and OVX+LO groups.
30862039	9	70	theme	total	1489:1493	arg1	TF					1500:1501	TF	1500:1501	TF	1500:1501	Our findings revealed that the body weight and relative tissues of UFP (uterus fatty peripheral) and total fat (TF) were significantly decreased in the OVX+TO group compared with those in the OVX+SO and OVX+LO groups.
30862039	10	71	theme	relative	1666:1673	arg1	tissues					1675:1681	the relative tissues	1662:1681	the relative tissues of UFP and TF	1662:1695	However, no significant difference in body weight or in the relative tissues of UFP and TF was noted among the OVX+SO and OVX+LO groups.
30862039	6	72	theme	female	1056:1061	arg1	Institute					1063:1071	7-week-old female Institute	1045:1071	7-week-old female Institute of Cancer Research (ICR) mice	1045:1101	In this study, 7-week-old female Institute of Cancer Research (ICR) mice underwent either bilateral laparotomy (sham group, n = 8) or bilateral oophorectomy (OVX groups, n = 24).
30862039	4	73	theme	fatty	726:730	arg1	PUFA					739:742	PUFA	739:742	PUFA	739:742	SO, TO, and LO comprise mainly polyunsaturated fatty acids (PUFA), monounsaturated fatty acids (MUFA), and saturated fatty acids (SFA), respectively.
30862039	4	73	theme	fatty	726:730	arg1	acids					732:736	polyunsaturated fatty acids	710:736	polyunsaturated fatty acids (PUFA)	710:743	SO, TO, and LO comprise mainly polyunsaturated fatty acids (PUFA), monounsaturated fatty acids (MUFA), and saturated fatty acids (SFA), respectively.
30862039	11	74	theme	liver	1835:1839	arg1	droplets					1847:1854	liver lipid droplets	1835:1854	liver lipid droplets	1835:1854	Furthermore, mice given an HFD rich in TO exhibited significantly decreased accumulation of liver lipid droplets and adipocyte sizes of UFP and brown adipose tissue (BAT) compared with those given an HFD rich in SO or LO.
30862039	12	75	theme	increased	2016:2024	arg1	tolerance					2039:2047	significantly increased oral glucose tolerance	2002:2047	significantly increased oral glucose tolerance	2002:2047	Moreover, replacing SO or LO with TO significantly increased oral glucose tolerance.
30862039	3	76	theme	lard	627:630	arg1	LO					637:638	LO	637:638	LO	637:638	In the present study, we investigated the effect of long-term feeding of edible oils (soybean oil (SO), tea seed oil (TO), and lard oil (LO)) on female ovariectomized (OVX) mice.
30862039	3	76	theme	lard	627:630	arg1	oil					632:634	lard oil	627:634	lard oil (LO)	627:639	In the present study, we investigated the effect of long-term feeding of edible oils (soybean oil (SO), tea seed oil (TO), and lard oil (LO)) on female ovariectomized (OVX) mice.
30862039	3	76	theme	lard	627:630	arg1	oils					580:583	edible oils	573:583	edible oils (soybean oil (SO), tea seed oil (TO), and lard oil (LO))	573:640	In the present study, we investigated the effect of long-term feeding of edible oils (soybean oil (SO), tea seed oil (TO), and lard oil (LO)) on female ovariectomized (OVX) mice.
30862039	14	77	theme	obese	2395:2399	arg1	model					2410:2414	this HFD-induced obese OVX mice model	2378:2414	this HFD-induced obese OVX mice model	2378:2414	Thus, tea seed oil (TO) rich in MUFA could prevent obesity, reduce physical fatigue, and improve exercise performance compared with either SO (PUFA)- or LO(SFA)-rich diets in this HFD-induced obese OVX mice model.
30862039	12	78	theme	oral	2026:2029	arg1	tolerance					2039:2047	significantly increased oral glucose tolerance	2002:2047	significantly increased oral glucose tolerance	2002:2047	Moreover, replacing SO or LO with TO significantly increased oral glucose tolerance.
30862039	1	79	theme	body	183:186	arg1	composition					188:198	body composition	183:198	body composition (a decline in lean body mass and an increase in total fat mass)	183:262	Menopause is associated with changes in body composition (a decline in lean body mass and an increase in total fat mass), leading to an increased risk of metabolic syndrome, nonalcoholic fatty liver disease, and heart disease.
30862039	10	80	theme	OVX+SO	1717:1722	arg1	groups					1735:1740	the OVX+SO and OVX+LO groups	1713:1740	groups	1735:1740	However, no significant difference in body weight or in the relative tissues of UFP and TF was noted among the OVX+SO and OVX+LO groups.
30862039	0	81	theme	Ovariectomized	122:135	arg1	Mice					137:140	High-Fat-Diet-Induced Obese Ovariectomized Mice	94:140	High-Fat-Diet-Induced Obese Ovariectomized Mice	94:140	Tea Seed Oil Prevents Obesity, Reduces Physical Fatigue, and Improves Exercise Performance in High-Fat-Diet-Induced Obese Ovariectomized Mice.
30862039	8	82	theme	OVX	1365:1367	arg1	mice					1369:1372	the OVX mice	1361:1372	the OVX mice	1361:1372	An HFD rich in SO, TO, or LO was given to the OVX mice for 12 weeks.
30862039	4	83	theme	monounsaturated	746:760	arg1	MUFA					775:778	MUFA	775:778	MUFA	775:778	SO, TO, and LO comprise mainly polyunsaturated fatty acids (PUFA), monounsaturated fatty acids (MUFA), and saturated fatty acids (SFA), respectively.
30862039	4	83	theme	monounsaturated	746:760	arg1	acids					768:772	monounsaturated fatty acids	746:772	monounsaturated fatty acids (MUFA)	746:779	SO, TO, and LO comprise mainly polyunsaturated fatty acids (PUFA), monounsaturated fatty acids (MUFA), and saturated fatty acids (SFA), respectively.
30862039	9	84	theme	OVX+TO	1540:1545	arg1	group					1547:1551	the OVX+TO group	1536:1551	the OVX+TO group	1536:1551	Our findings revealed that the body weight and relative tissues of UFP (uterus fatty peripheral) and total fat (TF) were significantly decreased in the OVX+TO group compared with those in the OVX+SO and OVX+LO groups.
30862039	11	85	theme	adipocyte	1860:1868	arg1	sizes					1870:1874	adipocyte sizes	1860:1874	adipocyte sizes of UFP and brown adipose tissue (BAT)	1860:1912	Furthermore, mice given an HFD rich in TO exhibited significantly decreased accumulation of liver lipid droplets and adipocyte sizes of UFP and brown adipose tissue (BAT) compared with those given an HFD rich in SO or LO.
30862039	6	86	theme	mice	1098:1101	arg1	Institute					1063:1071	7-week-old female Institute	1045:1071	7-week-old female Institute of Cancer Research (ICR) mice	1045:1101	In this study, 7-week-old female Institute of Cancer Research (ICR) mice underwent either bilateral laparotomy (sham group, n = 8) or bilateral oophorectomy (OVX groups, n = 24).
30862039	7	87	dep	groups	1283:1288	arg1	groups					1283:1288	three groups	1277:1288	three groups: OVX+SO, OVX+TO, and OVX+LO	1277:1316	The OVX mice given a high-fat diet (HFD) were randomly divided into three groups: OVX+SO, OVX+TO, and OVX+LO.
30862039	7	87	dep	groups	1283:1288	arg1	OVX+TO					1299:1304	OVX+TO	1299:1304	OVX+TO	1299:1304	The OVX mice given a high-fat diet (HFD) were randomly divided into three groups: OVX+SO, OVX+TO, and OVX+LO.
30862039	7	87	dep	groups	1283:1288	arg1	OVX+LO					1311:1316	OVX+LO	1311:1316	OVX+LO	1311:1316	The OVX mice given a high-fat diet (HFD) were randomly divided into three groups: OVX+SO, OVX+TO, and OVX+LO.
30862039	7	87	dep	groups	1283:1288	arg1	OVX+SO					1291:1296	OVX+SO	1291:1296	OVX+SO	1291:1296	The OVX mice given a high-fat diet (HFD) were randomly divided into three groups: OVX+SO, OVX+TO, and OVX+LO.
30862039	9	88	dep	OVX+SO	1580:1585	arg1	groups					1598:1603	groups	1598:1603	groups	1598:1603	Our findings revealed that the body weight and relative tissues of UFP (uterus fatty peripheral) and total fat (TF) were significantly decreased in the OVX+TO group compared with those in the OVX+SO and OVX+LO groups.
30862039	9	88	dep	OVX+SO	1580:1585	arg1	the					1576:1578	the	1576:1578	the	1576:1578	Our findings revealed that the body weight and relative tissues of UFP (uterus fatty peripheral) and total fat (TF) were significantly decreased in the OVX+TO group compared with those in the OVX+SO and OVX+LO groups.
30862039	14	89	theme	OVX	2401:2403	arg1	model					2410:2414	this HFD-induced obese OVX mice model	2378:2414	this HFD-induced obese OVX mice model	2378:2414	Thus, tea seed oil (TO) rich in MUFA could prevent obesity, reduce physical fatigue, and improve exercise performance compared with either SO (PUFA)- or LO(SFA)-rich diets in this HFD-induced obese OVX mice model.
30862039	3	90	theme	ovariectomized	652:665	arg1	mice					673:676	female ovariectomized (OVX) mice	645:676	female ovariectomized (OVX) mice	645:676	In the present study, we investigated the effect of long-term feeding of edible oils (soybean oil (SO), tea seed oil (TO), and lard oil (LO)) on female ovariectomized (OVX) mice.
30862039	6	91	theme	ICR	1093:1095	arg1	mice					1098:1101	Cancer Research (ICR) mice	1076:1101	Cancer Research (ICR) mice	1076:1101	In this study, 7-week-old female Institute of Cancer Research (ICR) mice underwent either bilateral laparotomy (sham group, n = 8) or bilateral oophorectomy (OVX groups, n = 24).
30862039	1	92	theme	heart	355:359	arg1	disease					361:367	heart disease	355:367	heart disease	355:367	Menopause is associated with changes in body composition (a decline in lean body mass and an increase in total fat mass), leading to an increased risk of metabolic syndrome, nonalcoholic fatty liver disease, and heart disease.
30862039	5	93	from	homeostasis	990:1000	arg1	population					1018:1027	a menopausal population	1005:1027	a menopausal population	1005:1027	However, there have been quite limited studies to investigate the effects of different fatty acids (PUFA, MUFA, and SFA) on physiological adaption and metabolic homeostasis in a menopausal population.
30862039	10	94	theme	OVX+LO	1728:1733	arg1	groups					1735:1740	the OVX+SO and OVX+LO groups	1713:1740	groups	1735:1740	However, no significant difference in body weight or in the relative tissues of UFP and TF was noted among the OVX+SO and OVX+LO groups.
30862039	6	95	theme	Research	1083:1090	arg1	mice					1098:1101	Cancer Research (ICR) mice	1076:1101	Cancer Research (ICR) mice	1076:1101	In this study, 7-week-old female Institute of Cancer Research (ICR) mice underwent either bilateral laparotomy (sham group, n = 8) or bilateral oophorectomy (OVX groups, n = 24).
30862039	3	96	theme	edible	573:578	arg1	oil					594:596	soybean oil	586:596	soybean oil (SO)	586:601	In the present study, we investigated the effect of long-term feeding of edible oils (soybean oil (SO), tea seed oil (TO), and lard oil (LO)) on female ovariectomized (OVX) mice.
30862039	3	96	theme	edible	573:578	arg1	oil					632:634	lard oil	627:634	lard oil (LO)	627:639	In the present study, we investigated the effect of long-term feeding of edible oils (soybean oil (SO), tea seed oil (TO), and lard oil (LO)) on female ovariectomized (OVX) mice.
30862039	3	96	theme	edible	573:578	arg1	oil					613:615	tea seed oil	604:615	tea seed oil (TO)	604:620	In the present study, we investigated the effect of long-term feeding of edible oils (soybean oil (SO), tea seed oil (TO), and lard oil (LO)) on female ovariectomized (OVX) mice.
30862039	3	96	theme	edible	573:578	arg1	oils					580:583	edible oils	573:583	edible oils (soybean oil (SO), tea seed oil (TO), and lard oil (LO))	573:640	In the present study, we investigated the effect of long-term feeding of edible oils (soybean oil (SO), tea seed oil (TO), and lard oil (LO)) on female ovariectomized (OVX) mice.
30862039	0	97	from	Performance	79:89	arg1	Mice					137:140	High-Fat-Diet-Induced Obese Ovariectomized Mice	94:140	High-Fat-Diet-Induced Obese Ovariectomized Mice	94:140	Tea Seed Oil Prevents Obesity, Reduces Physical Fatigue, and Improves Exercise Performance in High-Fat-Diet-Induced Obese Ovariectomized Mice.
30862039	14	98	theme	physical	2270:2277	arg1	fatigue					2279:2285	physical fatigue	2270:2285	physical fatigue	2270:2285	Thus, tea seed oil (TO) rich in MUFA could prevent obesity, reduce physical fatigue, and improve exercise performance compared with either SO (PUFA)- or LO(SFA)-rich diets in this HFD-induced obese OVX mice model.
30862039	5	99	theme	limited	860:866	arg1	studies					868:874	quite limited studies	854:874	quite limited studies	854:874	However, there have been quite limited studies to investigate the effects of different fatty acids (PUFA, MUFA, and SFA) on physiological adaption and metabolic homeostasis in a menopausal population.
30862039	3	100	theme	feeding	562:568	arg1	effect					542:547	the effect	538:547	the effect of long-term feeding of edible oils (soybean oil (SO), tea seed oil (TO), and lard oil (LO)) on female ovariectomized (OVX) mice	538:676	In the present study, we investigated the effect of long-term feeding of edible oils (soybean oil (SO), tea seed oil (TO), and lard oil (LO)) on female ovariectomized (OVX) mice.
30862039	4	101	theme	saturated	786:794	arg1	SFA					809:811	SFA	809:811	SFA	809:811	SO, TO, and LO comprise mainly polyunsaturated fatty acids (PUFA), monounsaturated fatty acids (MUFA), and saturated fatty acids (SFA), respectively.
30862039	4	101	theme	saturated	786:794	arg1	acids					802:806	saturated fatty acids	786:806	saturated fatty acids (SFA)	786:812	SO, TO, and LO comprise mainly polyunsaturated fatty acids (PUFA), monounsaturated fatty acids (MUFA), and saturated fatty acids (SFA), respectively.
30862039	11	102	theme	decreased	1809:1817	arg1	accumulation					1819:1830	significantly decreased accumulation	1795:1830	significantly decreased accumulation of liver lipid droplets	1795:1854	Furthermore, mice given an HFD rich in TO exhibited significantly decreased accumulation of liver lipid droplets and adipocyte sizes of UFP and brown adipose tissue (BAT) compared with those given an HFD rich in SO or LO.
30862039	13	103	theme	creatine	2179:2186	arg1	kinase					2188:2193	creatine kinase	2179:2193	creatine kinase	2179:2193	Additionally, TO improved endurance performance and exhibited antifatigue activity by lowering ammonia, blood urea nitrogen, and creatine kinase levels.
30862039	6	104	dep	laparotomy	1130:1139	arg1	=					1156:1156	=	1156:1156	=	1156:1156	In this study, 7-week-old female Institute of Cancer Research (ICR) mice underwent either bilateral laparotomy (sham group, n = 8) or bilateral oophorectomy (OVX groups, n = 24).
30862039	6	105	theme	sham	1142:1145	arg1	group					1147:1151	sham group	1142:1151	sham group	1142:1151	In this study, 7-week-old female Institute of Cancer Research (ICR) mice underwent either bilateral laparotomy (sham group, n = 8) or bilateral oophorectomy (OVX groups, n = 24).
30862039	1	106	theme	syndrome	307:314	arg1	risk					289:292	an increased risk	276:292	an increased risk of metabolic syndrome, nonalcoholic fatty liver disease, and heart disease	276:367	Menopause is associated with changes in body composition (a decline in lean body mass and an increase in total fat mass), leading to an increased risk of metabolic syndrome, nonalcoholic fatty liver disease, and heart disease.
30862039	2	107	theme	metabolic	479:487	arg1	syndromes					489:497	menopause-related metabolic syndromes	461:497	menopause-related metabolic syndromes	461:497	A healthy diet to control body weight is an effective strategy for preventing and treating menopause-related metabolic syndromes.
30862039	11	108	theme	droplets	1847:1854	arg1	accumulation					1819:1830	significantly decreased accumulation	1795:1830	significantly decreased accumulation of liver lipid droplets	1795:1854	Furthermore, mice given an HFD rich in TO exhibited significantly decreased accumulation of liver lipid droplets and adipocyte sizes of UFP and brown adipose tissue (BAT) compared with those given an HFD rich in SO or LO.
30862039	11	108	theme	droplets	1847:1854	arg1	sizes					1870:1874	adipocyte sizes	1860:1874	adipocyte sizes of UFP and brown adipose tissue (BAT)	1860:1912	Furthermore, mice given an HFD rich in TO exhibited significantly decreased accumulation of liver lipid droplets and adipocyte sizes of UFP and brown adipose tissue (BAT) compared with those given an HFD rich in SO or LO.
30862039	0	109	theme	High-Fat-Diet-Induced	94:114	arg1	Mice					137:140	High-Fat-Diet-Induced Obese Ovariectomized Mice	94:140	High-Fat-Diet-Induced Obese Ovariectomized Mice	94:140	Tea Seed Oil Prevents Obesity, Reduces Physical Fatigue, and Improves Exercise Performance in High-Fat-Diet-Induced Obese Ovariectomized Mice.
30862039	1	110	theme	nonalcoholic	317:328	arg1	disease					342:348	nonalcoholic fatty liver disease	317:348	nonalcoholic fatty liver disease	317:348	Menopause is associated with changes in body composition (a decline in lean body mass and an increase in total fat mass), leading to an increased risk of metabolic syndrome, nonalcoholic fatty liver disease, and heart disease.
30862039	9	111	theme	body	1419:1422	arg1	weight					1424:1429	body weight	1419:1429	body weight	1419:1429	Our findings revealed that the body weight and relative tissues of UFP (uterus fatty peripheral) and total fat (TF) were significantly decreased in the OVX+TO group compared with those in the OVX+SO and OVX+LO groups.
30862039	1	112	theme	body	219:222	arg1	mass					224:227	lean body mass	214:227	lean body mass	214:227	Menopause is associated with changes in body composition (a decline in lean body mass and an increase in total fat mass), leading to an increased risk of metabolic syndrome, nonalcoholic fatty liver disease, and heart disease.
30862039	13	113	theme	urea	2160:2163	arg1	nitrogen					2165:2172	blood urea nitrogen	2154:2172	blood urea nitrogen	2154:2172	Additionally, TO improved endurance performance and exhibited antifatigue activity by lowering ammonia, blood urea nitrogen, and creatine kinase levels.
30862039	0	114	theme	Seed	4:7	arg1	Oil					9:11	Tea Seed Oil	0:11	Tea Seed Oil	0:11	Tea Seed Oil Prevents Obesity, Reduces Physical Fatigue, and Improves Exercise Performance in High-Fat-Diet-Induced Obese Ovariectomized Mice.
30862039	3	115	dep	ovariectomized	652:665	arg1	OVX					668:670	OVX	668:670	OVX	668:670	In the present study, we investigated the effect of long-term feeding of edible oils (soybean oil (SO), tea seed oil (TO), and lard oil (LO)) on female ovariectomized (OVX) mice.
30862039	6	116	dep	=	1156:1156	arg1	group					1147:1151	sham group	1142:1151	sham group	1142:1151	In this study, 7-week-old female Institute of Cancer Research (ICR) mice underwent either bilateral laparotomy (sham group, n = 8) or bilateral oophorectomy (OVX groups, n = 24).
30862039	5	117	from	effects	895:901	arg1	homeostasis					990:1000	metabolic homeostasis	980:1000	metabolic homeostasis	980:1000	However, there have been quite limited studies to investigate the effects of different fatty acids (PUFA, MUFA, and SFA) on physiological adaption and metabolic homeostasis in a menopausal population.
30862039	5	117	from	effects	895:901	arg1	adaption					967:974	physiological adaption	953:974	physiological adaption	953:974	However, there have been quite limited studies to investigate the effects of different fatty acids (PUFA, MUFA, and SFA) on physiological adaption and metabolic homeostasis in a menopausal population.
30862039	5	118	dep	acids	922:926	arg1	MUFA					935:938	MUFA	935:938	MUFA	935:938	However, there have been quite limited studies to investigate the effects of different fatty acids (PUFA, MUFA, and SFA) on physiological adaption and metabolic homeostasis in a menopausal population.
30862039	5	118	dep	acids	922:926	arg1	SFA					945:947	SFA	945:947	SFA	945:947	However, there have been quite limited studies to investigate the effects of different fatty acids (PUFA, MUFA, and SFA) on physiological adaption and metabolic homeostasis in a menopausal population.
30862039	5	118	dep	acids	922:926	arg1	PUFA					929:932	PUFA	929:932	PUFA	929:932	However, there have been quite limited studies to investigate the effects of different fatty acids (PUFA, MUFA, and SFA) on physiological adaption and metabolic homeostasis in a menopausal population.
30862039	5	118	dep	acids	922:926	arg1	acids					922:926	different fatty acids	906:926	different fatty acids (PUFA, MUFA, and SFA)	906:948	However, there have been quite limited studies to investigate the effects of different fatty acids (PUFA, MUFA, and SFA) on physiological adaption and metabolic homeostasis in a menopausal population.
30862039	11	119	from	SO	1955:1956	arg1	rich					1947:1950	rich	1947:1950	rich	1947:1950	Furthermore, mice given an HFD rich in TO exhibited significantly decreased accumulation of liver lipid droplets and adipocyte sizes of UFP and brown adipose tissue (BAT) compared with those given an HFD rich in SO or LO.
30862039	6	120	theme	bilateral	1164:1172	arg1	oophorectomy					1174:1185	bilateral oophorectomy	1164:1185	bilateral oophorectomy (OVX groups, n = 24)	1164:1206	In this study, 7-week-old female Institute of Cancer Research (ICR) mice underwent either bilateral laparotomy (sham group, n = 8) or bilateral oophorectomy (OVX groups, n = 24).
30862039	11	121	theme	UFP	1879:1881	arg1	accumulation					1819:1830	significantly decreased accumulation	1795:1830	significantly decreased accumulation of liver lipid droplets	1795:1854	Furthermore, mice given an HFD rich in TO exhibited significantly decreased accumulation of liver lipid droplets and adipocyte sizes of UFP and brown adipose tissue (BAT) compared with those given an HFD rich in SO or LO.
30862039	11	121	theme	UFP	1879:1881	arg1	sizes					1870:1874	adipocyte sizes	1860:1874	adipocyte sizes of UFP and brown adipose tissue (BAT)	1860:1912	Furthermore, mice given an HFD rich in TO exhibited significantly decreased accumulation of liver lipid droplets and adipocyte sizes of UFP and brown adipose tissue (BAT) compared with those given an HFD rich in SO or LO.
30862039	1	122	theme	liver	336:340	arg1	disease					342:348	nonalcoholic fatty liver disease	317:348	nonalcoholic fatty liver disease	317:348	Menopause is associated with changes in body composition (a decline in lean body mass and an increase in total fat mass), leading to an increased risk of metabolic syndrome, nonalcoholic fatty liver disease, and heart disease.
30862039	1	123	dep	composition	188:198	arg1	increase					236:243	an increase	233:243	an increase in total fat mass	233:261	Menopause is associated with changes in body composition (a decline in lean body mass and an increase in total fat mass), leading to an increased risk of metabolic syndrome, nonalcoholic fatty liver disease, and heart disease.
30862039	1	123	dep	composition	188:198	arg1	decline					203:209	a decline	201:209	a decline in lean body mass	201:227	Menopause is associated with changes in body composition (a decline in lean body mass and an increase in total fat mass), leading to an increased risk of metabolic syndrome, nonalcoholic fatty liver disease, and heart disease.
30862039	11	124	from	LO	1961:1962	arg1	rich					1947:1950	rich	1947:1950	rich	1947:1950	Furthermore, mice given an HFD rich in TO exhibited significantly decreased accumulation of liver lipid droplets and adipocyte sizes of UFP and brown adipose tissue (BAT) compared with those given an HFD rich in SO or LO.
30862039	0	125	theme	Physical	39:46	arg1	Fatigue					48:54	Physical Fatigue	39:54	Physical Fatigue	39:54	Tea Seed Oil Prevents Obesity, Reduces Physical Fatigue, and Improves Exercise Performance in High-Fat-Diet-Induced Obese Ovariectomized Mice.
30862039	5	126	theme	different	906:914	arg1	MUFA					935:938	MUFA	935:938	MUFA	935:938	However, there have been quite limited studies to investigate the effects of different fatty acids (PUFA, MUFA, and SFA) on physiological adaption and metabolic homeostasis in a menopausal population.
30862039	5	126	theme	different	906:914	arg1	SFA					945:947	SFA	945:947	SFA	945:947	However, there have been quite limited studies to investigate the effects of different fatty acids (PUFA, MUFA, and SFA) on physiological adaption and metabolic homeostasis in a menopausal population.
30862039	5	126	theme	different	906:914	arg1	PUFA					929:932	PUFA	929:932	PUFA	929:932	However, there have been quite limited studies to investigate the effects of different fatty acids (PUFA, MUFA, and SFA) on physiological adaption and metabolic homeostasis in a menopausal population.
30862039	5	126	theme	different	906:914	arg1	acids					922:926	different fatty acids	906:926	different fatty acids (PUFA, MUFA, and SFA)	906:948	However, there have been quite limited studies to investigate the effects of different fatty acids (PUFA, MUFA, and SFA) on physiological adaption and metabolic homeostasis in a menopausal population.
30862039	11	127	dep	exhibited	1785:1793	arg1	TO					1782:1783	TO	1782:1783	TO	1782:1783	Furthermore, mice given an HFD rich in TO exhibited significantly decreased accumulation of liver lipid droplets and adipocyte sizes of UFP and brown adipose tissue (BAT) compared with those given an HFD rich in SO or LO.
30862039	1	128	theme	increased	279:287	arg1	risk					289:292	an increased risk	276:292	an increased risk of metabolic syndrome, nonalcoholic fatty liver disease, and heart disease	276:367	Menopause is associated with changes in body composition (a decline in lean body mass and an increase in total fat mass), leading to an increased risk of metabolic syndrome, nonalcoholic fatty liver disease, and heart disease.
30862039	14	129	dep	diets	2369:2373	arg1	-rich					2363:2367	-rich	2363:2367	-rich	2363:2367	Thus, tea seed oil (TO) rich in MUFA could prevent obesity, reduce physical fatigue, and improve exercise performance compared with either SO (PUFA)- or LO(SFA)-rich diets in this HFD-induced obese OVX mice model.
30862039	7	130	theme	high-fat	1230:1237	arg1	HFD					1245:1247	HFD	1245:1247	HFD	1245:1247	The OVX mice given a high-fat diet (HFD) were randomly divided into three groups: OVX+SO, OVX+TO, and OVX+LO.
30862039	7	130	theme	high-fat	1230:1237	arg1	diet					1239:1242	a high-fat diet	1228:1242	a high-fat diet (HFD)	1228:1248	The OVX mice given a high-fat diet (HFD) were randomly divided into three groups: OVX+SO, OVX+TO, and OVX+LO.
30862039	6	131	dep	=	1202:1202	arg1	groups					1192:1197	OVX groups	1188:1197	OVX groups	1188:1197	In this study, 7-week-old female Institute of Cancer Research (ICR) mice underwent either bilateral laparotomy (sham group, n = 8) or bilateral oophorectomy (OVX groups, n = 24).
30862039	14	132	theme	seed	2213:2216	arg1	oil					2218:2220	tea seed oil	2209:2220	tea seed oil (TO) rich in MUFA	2209:2238	Thus, tea seed oil (TO) rich in MUFA could prevent obesity, reduce physical fatigue, and improve exercise performance compared with either SO (PUFA)- or LO(SFA)-rich diets in this HFD-induced obese OVX mice model.
30862039	11	133	theme	brown	1887:1891	arg1	tissue					1901:1906	brown adipose tissue	1887:1906	brown adipose tissue (BAT)	1887:1912	Furthermore, mice given an HFD rich in TO exhibited significantly decreased accumulation of liver lipid droplets and adipocyte sizes of UFP and brown adipose tissue (BAT) compared with those given an HFD rich in SO or LO.
30862039	11	133	theme	brown	1887:1891	arg1	BAT					1909:1911	BAT	1909:1911	BAT	1909:1911	Furthermore, mice given an HFD rich in TO exhibited significantly decreased accumulation of liver lipid droplets and adipocyte sizes of UFP and brown adipose tissue (BAT) compared with those given an HFD rich in SO or LO.
30862039	11	134	from	rich	1947:1950	arg1	LO					1961:1962	LO	1961:1962	LO	1961:1962	Furthermore, mice given an HFD rich in TO exhibited significantly decreased accumulation of liver lipid droplets and adipocyte sizes of UFP and brown adipose tissue (BAT) compared with those given an HFD rich in SO or LO.
30862039	11	134	from	rich	1947:1950	arg1	SO					1955:1956	SO	1955:1956	SO	1955:1956	Furthermore, mice given an HFD rich in TO exhibited significantly decreased accumulation of liver lipid droplets and adipocyte sizes of UFP and brown adipose tissue (BAT) compared with those given an HFD rich in SO or LO.
30862039	5	135	theme	menopausal	1007:1016	arg1	population					1018:1027	a menopausal population	1005:1027	a menopausal population	1005:1027	However, there have been quite limited studies to investigate the effects of different fatty acids (PUFA, MUFA, and SFA) on physiological adaption and metabolic homeostasis in a menopausal population.
30862039	10	136	theme	body	1644:1647	arg1	weight					1649:1654	body weight	1644:1654	body weight	1644:1654	However, no significant difference in body weight or in the relative tissues of UFP and TF was noted among the OVX+SO and OVX+LO groups.
30862039	13	137	dep	improved	2067:2074	arg1	TO					2064:2065	TO	2064:2065	TO	2064:2065	Additionally, TO improved endurance performance and exhibited antifatigue activity by lowering ammonia, blood urea nitrogen, and creatine kinase levels.
31502452	0	0	theme	Cargo	86:90	arg1	Encapsulation					46:58	Encapsulation	46:58	Encapsulation	46:58	Phosphonium Polyelectrolyte Complexes for the Encapsulation and Slow Release of Ionic Cargo.
31502452	0	0	theme	Cargo	86:90	arg1	Release					69:75	Slow Release	64:75	Slow Release	64:75	Phosphonium Polyelectrolyte Complexes for the Encapsulation and Slow Release of Ionic Cargo.
31502452	2	1	theme	mechanical	448:457	arg1	properties					459:468	weak, fluid-like mechanical properties	431:468	weak, fluid-like mechanical properties	431:468	These networks can be used to encapsulate and release cargo, but the release of cargo is typically rapid, occurring over a period of hours to a few days and they often exhibit weak, fluid-like mechanical properties.
31502452	8	2	theme	polyphosphonium-HA	1763:1780	arg1	networks					1782:1789	polyphosphonium-HA networks	1763:1789	polyphosphonium-HA networks	1763:1789	Overall, this work demonstrates the utility of polyphosphonium-HA networks for the loading and slow release of ionic drugs and that their physical and biological properties can be readily tuned according to the structure of the phosphonium polymer.
31502452	7	3	with	cytotoxicities	1510:1523	arg1	HA					1579:1580	HA	1579:1580	HA	1579:1580	The cytotoxicities of the polymers and their corresponding networks with HA to C2C12 mouse myoblast cells was investigated and found to depend on the structure of the polymer and the properties of the network.
31502452	1	4	theme	charged	171:177	arg1	polymers					179:186	anionically and cationically charged polymers	142:186	anionically and cationically charged polymers	142:186	Polyelectrolyte complexation, the combination of anionically and cationically charged polymers through ionic interactions, can be used to form hydrogel networks.
31502452	0	5	theme	Ionic	80:84	arg1	Cargo					86:90	Ionic Cargo	80:90	Ionic Cargo	80:90	Phosphonium Polyelectrolyte Complexes for the Encapsulation and Slow Release of Ionic Cargo.
31502452	5	6	theme	phosphonium	995:1005	arg1	polymer					1007:1013	the phosphonium polymer	991:1013	the phosphonium polymer	991:1013	Their properties depended on the structure of the phosphonium polymer and the salt concentration, but in general, they exhibited predominantly gel-like behavior with relaxation times greater than 40 s and self-healing over 2-18 h. Anionic molecules, including fluorescein, diclofenac, and adenosine-5'-triphosphate, were encapsulated into the PECs with high loading capacities of up to 16 wt %.
31502452	1	7	theme	polymers	179:186	arg1	complexation					109:120	Polyelectrolyte complexation	93:120	Polyelectrolyte complexation	93:120	Polyelectrolyte complexation, the combination of anionically and cationically charged polymers through ionic interactions, can be used to form hydrogel networks.
31502452	1	7	theme	polymers	179:186	arg1	combination					127:137	the combination	123:137	the combination of anionically and cationically charged polymers through ionic interactions	123:213	Polyelectrolyte complexation, the combination of anionically and cationically charged polymers through ionic interactions, can be used to form hydrogel networks.
31502452	3	8	theme	poly[triethyl	753:765	arg1	chloride					793:800	poly[triethyl(4-vinylbenzyl)phosphonium chloride	753:800	poly[triethyl(4-vinylbenzyl)phosphonium chloride	753:800	Here we report the preparation and study of polyelectrolyte complexes (PECs) from sodium hyaluronate (HA) and poly[tris(hydroxypropyl)(4-vinylbenzyl)phosphonium chloride], poly[triphenyl(4-vinylbenzyl)phosphonium chloride], poly[tri(n-butyl)(4-vinylbenzyl)phosphonium chloride], or poly[triethyl(4-vinylbenzyl)phosphonium chloride].
31502452	6	9	theme	dense	1487:1491	arg1	network					1497:1503	the dense PEC network	1483:1503	the dense PEC network	1483:1503	Fluorescein and diclofenac were slowly released over 60 days, which was attributed to a combination of hydrophobic and ionic interactions with the dense PEC network.
31502452	5	10	dep	16	1331:1332	arg1	to					1328:1329	to	1328:1329	to	1328:1329	Their properties depended on the structure of the phosphonium polymer and the salt concentration, but in general, they exhibited predominantly gel-like behavior with relaxation times greater than 40 s and self-healing over 2-18 h. Anionic molecules, including fluorescein, diclofenac, and adenosine-5'-triphosphate, were encapsulated into the PECs with high loading capacities of up to 16 wt %.
31502452	3	11	from	chloride	793:800	arg1	study					506:510	study	506:510	study	506:510	Here we report the preparation and study of polyelectrolyte complexes (PECs) from sodium hyaluronate (HA) and poly[tris(hydroxypropyl)(4-vinylbenzyl)phosphonium chloride], poly[triphenyl(4-vinylbenzyl)phosphonium chloride], poly[tri(n-butyl)(4-vinylbenzyl)phosphonium chloride], or poly[triethyl(4-vinylbenzyl)phosphonium chloride].
31502452	3	11	from	chloride	793:800	arg1	preparation					490:500	the preparation	486:500	the preparation	486:500	Here we report the preparation and study of polyelectrolyte complexes (PECs) from sodium hyaluronate (HA) and poly[tris(hydroxypropyl)(4-vinylbenzyl)phosphonium chloride], poly[triphenyl(4-vinylbenzyl)phosphonium chloride], poly[tri(n-butyl)(4-vinylbenzyl)phosphonium chloride], or poly[triethyl(4-vinylbenzyl)phosphonium chloride].
31502452	8	12	theme	biological	1867:1876	arg1	properties					1878:1887	their physical and biological properties	1848:1887	their physical and biological properties	1848:1887	Overall, this work demonstrates the utility of polyphosphonium-HA networks for the loading and slow release of ionic drugs and that their physical and biological properties can be readily tuned according to the structure of the phosphonium polymer.
31502452	5	13	theme	salt	1023:1026	arg1	concentration					1028:1040	the salt concentration	1019:1040	the salt concentration	1019:1040	Their properties depended on the structure of the phosphonium polymer and the salt concentration, but in general, they exhibited predominantly gel-like behavior with relaxation times greater than 40 s and self-healing over 2-18 h. Anionic molecules, including fluorescein, diclofenac, and adenosine-5'-triphosphate, were encapsulated into the PECs with high loading capacities of up to 16 wt %.
31502452	2	14	dep	weak	431:434	arg1	fluid-like					437:446	fluid-like	437:446	fluid-like	437:446	These networks can be used to encapsulate and release cargo, but the release of cargo is typically rapid, occurring over a period of hours to a few days and they often exhibit weak, fluid-like mechanical properties.
31502452	6	15	theme	interactions	1465:1476	arg1	combination					1428:1438	a combination	1426:1438	a combination of hydrophobic and ionic interactions with the dense PEC network	1426:1503	Fluorescein and diclofenac were slowly released over 60 days, which was attributed to a combination of hydrophobic and ionic interactions with the dense PEC network.
31502452	6	16	theme	hydrophobic	1443:1453	arg1	interactions					1465:1476	hydrophobic and ionic interactions	1443:1476	hydrophobic and ionic interactions with the dense PEC network	1443:1503	Fluorescein and diclofenac were slowly released over 60 days, which was attributed to a combination of hydrophobic and ionic interactions with the dense PEC network.
31502452	3	17	theme	phosphonium	672:682	arg1	HA					573:574	sodium hyaluronate (HA) and poly[tris(hydroxypropyl)(4-vinylbenzyl)phosphonium chloride]	553:640	HA	573:574	Here we report the preparation and study of polyelectrolyte complexes (PECs) from sodium hyaluronate (HA) and poly[tris(hydroxypropyl)(4-vinylbenzyl)phosphonium chloride], poly[triphenyl(4-vinylbenzyl)phosphonium chloride], poly[tri(n-butyl)(4-vinylbenzyl)phosphonium chloride], or poly[triethyl(4-vinylbenzyl)phosphonium chloride].
31502452	3	17	theme	phosphonium	672:682	arg1	chloride					684:691	poly[triphenyl(4-vinylbenzyl)phosphonium chloride]	643:692	poly[triphenyl(4-vinylbenzyl)phosphonium chloride]	643:692	Here we report the preparation and study of polyelectrolyte complexes (PECs) from sodium hyaluronate (HA) and poly[tris(hydroxypropyl)(4-vinylbenzyl)phosphonium chloride], poly[triphenyl(4-vinylbenzyl)phosphonium chloride], poly[tri(n-butyl)(4-vinylbenzyl)phosphonium chloride], or poly[triethyl(4-vinylbenzyl)phosphonium chloride].
31502452	3	18	from	HA	573:574	arg1	study					506:510	study	506:510	study	506:510	Here we report the preparation and study of polyelectrolyte complexes (PECs) from sodium hyaluronate (HA) and poly[tris(hydroxypropyl)(4-vinylbenzyl)phosphonium chloride], poly[triphenyl(4-vinylbenzyl)phosphonium chloride], poly[tri(n-butyl)(4-vinylbenzyl)phosphonium chloride], or poly[triethyl(4-vinylbenzyl)phosphonium chloride].
31502452	3	18	from	HA	573:574	arg1	preparation					490:500	the preparation	486:500	the preparation	486:500	Here we report the preparation and study of polyelectrolyte complexes (PECs) from sodium hyaluronate (HA) and poly[tris(hydroxypropyl)(4-vinylbenzyl)phosphonium chloride], poly[triphenyl(4-vinylbenzyl)phosphonium chloride], poly[tri(n-butyl)(4-vinylbenzyl)phosphonium chloride], or poly[triethyl(4-vinylbenzyl)phosphonium chloride].
31502452	8	19	theme	phosphonium	1944:1954	arg1	polymer					1956:1962	the phosphonium polymer	1940:1962	the phosphonium polymer	1940:1962	Overall, this work demonstrates the utility of polyphosphonium-HA networks for the loading and slow release of ionic drugs and that their physical and biological properties can be readily tuned according to the structure of the phosphonium polymer.
31502452	5	20	theme	wt	1334:1335	arg1	%					1337:1337	up to 16 wt %	1325:1337	up to 16 wt %	1325:1337	Their properties depended on the structure of the phosphonium polymer and the salt concentration, but in general, they exhibited predominantly gel-like behavior with relaxation times greater than 40 s and self-healing over 2-18 h. Anionic molecules, including fluorescein, diclofenac, and adenosine-5'-triphosphate, were encapsulated into the PECs with high loading capacities of up to 16 wt %.
31502452	7	21	theme	myoblast	1597:1604	arg1	cells					1606:1610	C2C12 mouse myoblast cells	1585:1610	C2C12 mouse myoblast cells	1585:1610	The cytotoxicities of the polymers and their corresponding networks with HA to C2C12 mouse myoblast cells was investigated and found to depend on the structure of the polymer and the properties of the network.
31502452	1	22	theme	ionic	196:200	arg1	interactions					202:213	ionic interactions	196:213	ionic interactions	196:213	Polyelectrolyte complexation, the combination of anionically and cationically charged polymers through ionic interactions, can be used to form hydrogel networks.
31502452	0	23	theme	Polyelectrolyte	12:26	arg1	Complexes					28:36	Phosphonium Polyelectrolyte Complexes	0:36	Phosphonium Polyelectrolyte Complexes for the Encapsulation and Slow Release of Ionic Cargo.	0:91	Phosphonium Polyelectrolyte Complexes for the Encapsulation and Slow Release of Ionic Cargo.
31502452	3	24	theme	4-vinylbenzyl	658:670	arg1	HA					573:574	sodium hyaluronate (HA) and poly[tris(hydroxypropyl)(4-vinylbenzyl)phosphonium chloride]	553:640	HA	573:574	Here we report the preparation and study of polyelectrolyte complexes (PECs) from sodium hyaluronate (HA) and poly[tris(hydroxypropyl)(4-vinylbenzyl)phosphonium chloride], poly[triphenyl(4-vinylbenzyl)phosphonium chloride], poly[tri(n-butyl)(4-vinylbenzyl)phosphonium chloride], or poly[triethyl(4-vinylbenzyl)phosphonium chloride].
31502452	3	24	theme	4-vinylbenzyl	658:670	arg1	chloride					684:691	poly[triphenyl(4-vinylbenzyl)phosphonium chloride]	643:692	poly[triphenyl(4-vinylbenzyl)phosphonium chloride]	643:692	Here we report the preparation and study of polyelectrolyte complexes (PECs) from sodium hyaluronate (HA) and poly[tris(hydroxypropyl)(4-vinylbenzyl)phosphonium chloride], poly[triphenyl(4-vinylbenzyl)phosphonium chloride], poly[tri(n-butyl)(4-vinylbenzyl)phosphonium chloride], or poly[triethyl(4-vinylbenzyl)phosphonium chloride].
31502452	8	25	theme	slow	1811:1814	arg1	release					1816:1822	slow release	1811:1822	slow release	1811:1822	Overall, this work demonstrates the utility of polyphosphonium-HA networks for the loading and slow release of ionic drugs and that their physical and biological properties can be readily tuned according to the structure of the phosphonium polymer.
31502452	5	26	theme	%	1337:1337	arg1	capacities					1311:1320	high loading capacities	1298:1320	high loading capacities of up to 16 wt %	1298:1337	Their properties depended on the structure of the phosphonium polymer and the salt concentration, but in general, they exhibited predominantly gel-like behavior with relaxation times greater than 40 s and self-healing over 2-18 h. Anionic molecules, including fluorescein, diclofenac, and adenosine-5'-triphosphate, were encapsulated into the PECs with high loading capacities of up to 16 wt %.
31502452	5	27	theme	concentration	1028:1040	arg1	structure					978:986	the structure	974:986	the structure of the phosphonium polymer and the salt concentration	974:1040	Their properties depended on the structure of the phosphonium polymer and the salt concentration, but in general, they exhibited predominantly gel-like behavior with relaxation times greater than 40 s and self-healing over 2-18 h. Anionic molecules, including fluorescein, diclofenac, and adenosine-5'-triphosphate, were encapsulated into the PECs with high loading capacities of up to 16 wt %.
31502452	0	28	theme	Phosphonium	0:10	arg1	Complexes					28:36	Phosphonium Polyelectrolyte Complexes	0:36	Phosphonium Polyelectrolyte Complexes for the Encapsulation and Slow Release of Ionic Cargo.	0:91	Phosphonium Polyelectrolyte Complexes for the Encapsulation and Slow Release of Ionic Cargo.
31502452	7	29	theme	networks	1565:1572	arg1	cytotoxicities					1510:1523	The cytotoxicities	1506:1523	The cytotoxicities of the polymers and their corresponding networks with HA to C2C12 mouse myoblast cells	1506:1610	The cytotoxicities of the polymers and their corresponding networks with HA to C2C12 mouse myoblast cells was investigated and found to depend on the structure of the polymer and the properties of the network.
31502452	5	30	theme	high	1298:1301	arg1	capacities					1311:1320	high loading capacities	1298:1320	high loading capacities of up to 16 wt %	1298:1337	Their properties depended on the structure of the phosphonium polymer and the salt concentration, but in general, they exhibited predominantly gel-like behavior with relaxation times greater than 40 s and self-healing over 2-18 h. Anionic molecules, including fluorescein, diclofenac, and adenosine-5'-triphosphate, were encapsulated into the PECs with high loading capacities of up to 16 wt %.
31502452	1	31	theme	Polyelectrolyte	93:107	arg1	complexation					109:120	Polyelectrolyte complexation	93:120	Polyelectrolyte complexation	93:120	Polyelectrolyte complexation, the combination of anionically and cationically charged polymers through ionic interactions, can be used to form hydrogel networks.
31502452	1	31	theme	Polyelectrolyte	93:107	arg1	combination					127:137	the combination	123:137	the combination of anionically and cationically charged polymers through ionic interactions	123:213	Polyelectrolyte complexation, the combination of anionically and cationically charged polymers through ionic interactions, can be used to form hydrogel networks.
31502452	7	32	theme	C2C12	1585:1589	arg1	cells					1606:1610	C2C12 mouse myoblast cells	1585:1610	C2C12 mouse myoblast cells	1585:1610	The cytotoxicities of the polymers and their corresponding networks with HA to C2C12 mouse myoblast cells was investigated and found to depend on the structure of the polymer and the properties of the network.
31502452	5	33	dep	h.	1173:1174	arg1	encapsulated					1266:1277	encapsulated	1266:1277	were encapsulated into the PECs with high loading capacities of up to 16 wt %	1261:1337	Their properties depended on the structure of the phosphonium polymer and the salt concentration, but in general, they exhibited predominantly gel-like behavior with relaxation times greater than 40 s and self-healing over 2-18 h. Anionic molecules, including fluorescein, diclofenac, and adenosine-5'-triphosphate, were encapsulated into the PECs with high loading capacities of up to 16 wt %.
31502452	3	34	theme	poly[tri	695:702	arg1	chloride					739:746	poly[tri(n-butyl)(4-vinylbenzyl)phosphonium chloride]	695:747	poly[tri(n-butyl)(4-vinylbenzyl)phosphonium chloride]	695:747	Here we report the preparation and study of polyelectrolyte complexes (PECs) from sodium hyaluronate (HA) and poly[tris(hydroxypropyl)(4-vinylbenzyl)phosphonium chloride], poly[triphenyl(4-vinylbenzyl)phosphonium chloride], poly[tri(n-butyl)(4-vinylbenzyl)phosphonium chloride], or poly[triethyl(4-vinylbenzyl)phosphonium chloride].
31502452	7	35	theme	corresponding	1551:1563	arg1	networks					1565:1572	their corresponding networks	1545:1572	their corresponding networks	1545:1572	The cytotoxicities of the polymers and their corresponding networks with HA to C2C12 mouse myoblast cells was investigated and found to depend on the structure of the polymer and the properties of the network.
31502452	7	36	theme	polymer	1673:1679	arg1	structure					1656:1664	the structure	1652:1664	the structure of the polymer	1652:1679	The cytotoxicities of the polymers and their corresponding networks with HA to C2C12 mouse myoblast cells was investigated and found to depend on the structure of the polymer and the properties of the network.
31502452	7	36	theme	polymer	1673:1679	arg1	properties					1689:1698	the properties	1685:1698	the properties of the network	1685:1713	The cytotoxicities of the polymers and their corresponding networks with HA to C2C12 mouse myoblast cells was investigated and found to depend on the structure of the polymer and the properties of the network.
31502452	3	37	theme	polyelectrolyte	515:529	arg1	complexes					531:539	polyelectrolyte complexes	515:539	polyelectrolyte complexes (PECs)	515:546	Here we report the preparation and study of polyelectrolyte complexes (PECs) from sodium hyaluronate (HA) and poly[tris(hydroxypropyl)(4-vinylbenzyl)phosphonium chloride], poly[triphenyl(4-vinylbenzyl)phosphonium chloride], poly[tri(n-butyl)(4-vinylbenzyl)phosphonium chloride], or poly[triethyl(4-vinylbenzyl)phosphonium chloride].
31502452	3	37	theme	polyelectrolyte	515:529	arg1	PECs					542:545	PECs	542:545	PECs	542:545	Here we report the preparation and study of polyelectrolyte complexes (PECs) from sodium hyaluronate (HA) and poly[tris(hydroxypropyl)(4-vinylbenzyl)phosphonium chloride], poly[triphenyl(4-vinylbenzyl)phosphonium chloride], poly[tri(n-butyl)(4-vinylbenzyl)phosphonium chloride], or poly[triethyl(4-vinylbenzyl)phosphonium chloride].
31502452	7	38	theme	mouse	1591:1595	arg1	cells					1606:1610	C2C12 mouse myoblast cells	1585:1610	C2C12 mouse myoblast cells	1585:1610	The cytotoxicities of the polymers and their corresponding networks with HA to C2C12 mouse myoblast cells was investigated and found to depend on the structure of the polymer and the properties of the network.
31502452	2	39	theme	few	399:401	arg1	days					403:406	a few days	397:406	a few days	397:406	These networks can be used to encapsulate and release cargo, but the release of cargo is typically rapid, occurring over a period of hours to a few days and they often exhibit weak, fluid-like mechanical properties.
31502452	3	40	theme	hydroxypropyl	591:603	arg1	chloride					632:639	sodium hyaluronate (HA) and poly[tris(hydroxypropyl)(4-vinylbenzyl)phosphonium chloride]	553:640	chloride	632:639	Here we report the preparation and study of polyelectrolyte complexes (PECs) from sodium hyaluronate (HA) and poly[tris(hydroxypropyl)(4-vinylbenzyl)phosphonium chloride], poly[triphenyl(4-vinylbenzyl)phosphonium chloride], poly[tri(n-butyl)(4-vinylbenzyl)phosphonium chloride], or poly[triethyl(4-vinylbenzyl)phosphonium chloride].
31502452	3	41	theme	complexes	531:539	arg1	study					506:510	study	506:510	study	506:510	Here we report the preparation and study of polyelectrolyte complexes (PECs) from sodium hyaluronate (HA) and poly[tris(hydroxypropyl)(4-vinylbenzyl)phosphonium chloride], poly[triphenyl(4-vinylbenzyl)phosphonium chloride], poly[tri(n-butyl)(4-vinylbenzyl)phosphonium chloride], or poly[triethyl(4-vinylbenzyl)phosphonium chloride].
31502452	3	41	theme	complexes	531:539	arg1	preparation					490:500	the preparation	486:500	the preparation	486:500	Here we report the preparation and study of polyelectrolyte complexes (PECs) from sodium hyaluronate (HA) and poly[tris(hydroxypropyl)(4-vinylbenzyl)phosphonium chloride], poly[triphenyl(4-vinylbenzyl)phosphonium chloride], poly[tri(n-butyl)(4-vinylbenzyl)phosphonium chloride], or poly[triethyl(4-vinylbenzyl)phosphonium chloride].
31502452	8	42	dep	loading	1799:1805	arg1	the					1795:1797	the	1795:1797	the	1795:1797	Overall, this work demonstrates the utility of polyphosphonium-HA networks for the loading and slow release of ionic drugs and that their physical and biological properties can be readily tuned according to the structure of the phosphonium polymer.
31502452	3	43	from	chloride	632:639	arg1	study					506:510	study	506:510	study	506:510	Here we report the preparation and study of polyelectrolyte complexes (PECs) from sodium hyaluronate (HA) and poly[tris(hydroxypropyl)(4-vinylbenzyl)phosphonium chloride], poly[triphenyl(4-vinylbenzyl)phosphonium chloride], poly[tri(n-butyl)(4-vinylbenzyl)phosphonium chloride], or poly[triethyl(4-vinylbenzyl)phosphonium chloride].
31502452	3	43	from	chloride	632:639	arg1	preparation					490:500	the preparation	486:500	the preparation	486:500	Here we report the preparation and study of polyelectrolyte complexes (PECs) from sodium hyaluronate (HA) and poly[tris(hydroxypropyl)(4-vinylbenzyl)phosphonium chloride], poly[triphenyl(4-vinylbenzyl)phosphonium chloride], poly[tri(n-butyl)(4-vinylbenzyl)phosphonium chloride], or poly[triethyl(4-vinylbenzyl)phosphonium chloride].
31502452	5	44	theme	greater	1128:1134	arg1	times					1122:1126	relaxation times	1111:1126	relaxation times greater than 40 s and self-healing over 2-18 h. Anionic molecules, including fluorescein, diclofenac, and adenosine-5'-triphosphate, were encapsulated into the PECs with high loading capacities of up to 16 wt %	1111:1337	Their properties depended on the structure of the phosphonium polymer and the salt concentration, but in general, they exhibited predominantly gel-like behavior with relaxation times greater than 40 s and self-healing over 2-18 h. Anionic molecules, including fluorescein, diclofenac, and adenosine-5'-triphosphate, were encapsulated into the PECs with high loading capacities of up to 16 wt %.
31502452	6	45	with	interactions	1465:1476	arg1	network					1497:1503	the dense PEC network	1483:1503	the dense PEC network	1483:1503	Fluorescein and diclofenac were slowly released over 60 days, which was attributed to a combination of hydrophobic and ionic interactions with the dense PEC network.
31502452	5	46	theme	polymer	1007:1013	arg1	structure					978:986	the structure	974:986	the structure of the phosphonium polymer and the salt concentration	974:1040	Their properties depended on the structure of the phosphonium polymer and the salt concentration, but in general, they exhibited predominantly gel-like behavior with relaxation times greater than 40 s and self-healing over 2-18 h. Anionic molecules, including fluorescein, diclofenac, and adenosine-5'-triphosphate, were encapsulated into the PECs with high loading capacities of up to 16 wt %.
31502452	3	47	theme	sodium	553:558	arg1	HA					573:574	sodium hyaluronate (HA) and poly[tris(hydroxypropyl)(4-vinylbenzyl)phosphonium chloride]	553:640	HA	573:574	Here we report the preparation and study of polyelectrolyte complexes (PECs) from sodium hyaluronate (HA) and poly[tris(hydroxypropyl)(4-vinylbenzyl)phosphonium chloride], poly[triphenyl(4-vinylbenzyl)phosphonium chloride], poly[tri(n-butyl)(4-vinylbenzyl)phosphonium chloride], or poly[triethyl(4-vinylbenzyl)phosphonium chloride].
31502452	3	47	theme	sodium	553:558	arg1	chloride					684:691	poly[triphenyl(4-vinylbenzyl)phosphonium chloride]	643:692	poly[triphenyl(4-vinylbenzyl)phosphonium chloride]	643:692	Here we report the preparation and study of polyelectrolyte complexes (PECs) from sodium hyaluronate (HA) and poly[tris(hydroxypropyl)(4-vinylbenzyl)phosphonium chloride], poly[triphenyl(4-vinylbenzyl)phosphonium chloride], poly[tri(n-butyl)(4-vinylbenzyl)phosphonium chloride], or poly[triethyl(4-vinylbenzyl)phosphonium chloride].
31502452	2	48	theme	hours	388:392	arg1	period					378:383	a period	376:383	a period of hours to a few days	376:406	These networks can be used to encapsulate and release cargo, but the release of cargo is typically rapid, occurring over a period of hours to a few days and they often exhibit weak, fluid-like mechanical properties.
31502452	2	49	theme	weak	431:434	arg1	properties					459:468	weak, fluid-like mechanical properties	431:468	weak, fluid-like mechanical properties	431:468	These networks can be used to encapsulate and release cargo, but the release of cargo is typically rapid, occurring over a period of hours to a few days and they often exhibit weak, fluid-like mechanical properties.
31502452	7	50	theme	polymers	1532:1539	arg1	cytotoxicities					1510:1523	The cytotoxicities	1506:1523	The cytotoxicities of the polymers and their corresponding networks with HA to C2C12 mouse myoblast cells	1506:1610	The cytotoxicities of the polymers and their corresponding networks with HA to C2C12 mouse myoblast cells was investigated and found to depend on the structure of the polymer and the properties of the network.
31502452	8	51	theme	ionic	1827:1831	arg1	drugs					1833:1837	ionic drugs	1827:1837	ionic drugs	1827:1837	Overall, this work demonstrates the utility of polyphosphonium-HA networks for the loading and slow release of ionic drugs and that their physical and biological properties can be readily tuned according to the structure of the phosphonium polymer.
31502452	3	52	theme	poly[tris	581:589	arg1	chloride					632:639	sodium hyaluronate (HA) and poly[tris(hydroxypropyl)(4-vinylbenzyl)phosphonium chloride]	553:640	chloride	632:639	Here we report the preparation and study of polyelectrolyte complexes (PECs) from sodium hyaluronate (HA) and poly[tris(hydroxypropyl)(4-vinylbenzyl)phosphonium chloride], poly[triphenyl(4-vinylbenzyl)phosphonium chloride], poly[tri(n-butyl)(4-vinylbenzyl)phosphonium chloride], or poly[triethyl(4-vinylbenzyl)phosphonium chloride].
31502452	3	53	theme	hyaluronate	560:570	arg1	HA					573:574	sodium hyaluronate (HA) and poly[tris(hydroxypropyl)(4-vinylbenzyl)phosphonium chloride]	553:640	HA	573:574	Here we report the preparation and study of polyelectrolyte complexes (PECs) from sodium hyaluronate (HA) and poly[tris(hydroxypropyl)(4-vinylbenzyl)phosphonium chloride], poly[triphenyl(4-vinylbenzyl)phosphonium chloride], poly[tri(n-butyl)(4-vinylbenzyl)phosphonium chloride], or poly[triethyl(4-vinylbenzyl)phosphonium chloride].
31502452	3	53	theme	hyaluronate	560:570	arg1	chloride					684:691	poly[triphenyl(4-vinylbenzyl)phosphonium chloride]	643:692	poly[triphenyl(4-vinylbenzyl)phosphonium chloride]	643:692	Here we report the preparation and study of polyelectrolyte complexes (PECs) from sodium hyaluronate (HA) and poly[tris(hydroxypropyl)(4-vinylbenzyl)phosphonium chloride], poly[triphenyl(4-vinylbenzyl)phosphonium chloride], poly[tri(n-butyl)(4-vinylbenzyl)phosphonium chloride], or poly[triethyl(4-vinylbenzyl)phosphonium chloride].
31502452	6	54	theme	ionic	1459:1463	arg1	interactions					1465:1476	hydrophobic and ionic interactions	1443:1476	hydrophobic and ionic interactions with the dense PEC network	1443:1503	Fluorescein and diclofenac were slowly released over 60 days, which was attributed to a combination of hydrophobic and ionic interactions with the dense PEC network.
31502452	0	55	theme	Slow	64:67	arg1	Release					69:75	Slow Release	64:75	Slow Release	64:75	Phosphonium Polyelectrolyte Complexes for the Encapsulation and Slow Release of Ionic Cargo.
31502452	8	56	theme	drugs	1833:1837	arg1	loading					1799:1805	loading	1799:1805	loading	1799:1805	Overall, this work demonstrates the utility of polyphosphonium-HA networks for the loading and slow release of ionic drugs and that their physical and biological properties can be readily tuned according to the structure of the phosphonium polymer.
31502452	8	56	theme	drugs	1833:1837	arg1	release					1816:1822	slow release	1811:1822	slow release	1811:1822	Overall, this work demonstrates the utility of polyphosphonium-HA networks for the loading and slow release of ionic drugs and that their physical and biological properties can be readily tuned according to the structure of the phosphonium polymer.
31502452	5	57	theme	relaxation	1111:1120	arg1	times					1122:1126	relaxation times	1111:1126	relaxation times greater than 40 s and self-healing over 2-18 h. Anionic molecules, including fluorescein, diclofenac, and adenosine-5'-triphosphate, were encapsulated into the PECs with high loading capacities of up to 16 wt %	1111:1337	Their properties depended on the structure of the phosphonium polymer and the salt concentration, but in general, they exhibited predominantly gel-like behavior with relaxation times greater than 40 s and self-healing over 2-18 h. Anionic molecules, including fluorescein, diclofenac, and adenosine-5'-triphosphate, were encapsulated into the PECs with high loading capacities of up to 16 wt %.
31502452	0	58	dep	Encapsulation	46:58	arg1	the					42:44	the	42:44	the	42:44	Phosphonium Polyelectrolyte Complexes for the Encapsulation and Slow Release of Ionic Cargo.
31502452	8	59	theme	networks	1782:1789	arg1	utility					1752:1758	the utility	1748:1758	the utility of polyphosphonium-HA networks for the loading and slow release of ionic drugs	1748:1837	Overall, this work demonstrates the utility of polyphosphonium-HA networks for the loading and slow release of ionic drugs and that their physical and biological properties can be readily tuned according to the structure of the phosphonium polymer.
31502452	2	60	theme	cargo	335:339	arg1	rapid					354:358	rapid	354:358	rapid	354:358	These networks can be used to encapsulate and release cargo, but the release of cargo is typically rapid, occurring over a period of hours to a few days and they often exhibit weak, fluid-like mechanical properties.
31502452	2	60	theme	cargo	335:339	arg1	release					324:330	the release	320:330	the release of cargo	320:339	These networks can be used to encapsulate and release cargo, but the release of cargo is typically rapid, occurring over a period of hours to a few days and they often exhibit weak, fluid-like mechanical properties.
31502452	8	61	theme	polymer	1956:1962	arg1	structure					1927:1935	the structure	1923:1935	the structure of the phosphonium polymer	1923:1962	Overall, this work demonstrates the utility of polyphosphonium-HA networks for the loading and slow release of ionic drugs and that their physical and biological properties can be readily tuned according to the structure of the phosphonium polymer.
31502452	5	62	theme	gel-like	1088:1095	arg1	behavior					1097:1104	predominantly gel-like behavior	1074:1104	predominantly gel-like behavior	1074:1104	Their properties depended on the structure of the phosphonium polymer and the salt concentration, but in general, they exhibited predominantly gel-like behavior with relaxation times greater than 40 s and self-healing over 2-18 h. Anionic molecules, including fluorescein, diclofenac, and adenosine-5'-triphosphate, were encapsulated into the PECs with high loading capacities of up to 16 wt %.
31502452	5	63	with	PECs	1288:1291	arg1	capacities					1311:1320	high loading capacities	1298:1320	high loading capacities of up to 16 wt %	1298:1337	Their properties depended on the structure of the phosphonium polymer and the salt concentration, but in general, they exhibited predominantly gel-like behavior with relaxation times greater than 40 s and self-healing over 2-18 h. Anionic molecules, including fluorescein, diclofenac, and adenosine-5'-triphosphate, were encapsulated into the PECs with high loading capacities of up to 16 wt %.
31502452	3	64	theme	n-butyl	704:710	arg1	chloride					739:746	poly[tri(n-butyl)(4-vinylbenzyl)phosphonium chloride]	695:747	poly[tri(n-butyl)(4-vinylbenzyl)phosphonium chloride]	695:747	Here we report the preparation and study of polyelectrolyte complexes (PECs) from sodium hyaluronate (HA) and poly[tris(hydroxypropyl)(4-vinylbenzyl)phosphonium chloride], poly[triphenyl(4-vinylbenzyl)phosphonium chloride], poly[tri(n-butyl)(4-vinylbenzyl)phosphonium chloride], or poly[triethyl(4-vinylbenzyl)phosphonium chloride].
31502452	8	65	theme	physical	1854:1861	arg1	properties					1878:1887	their physical and biological properties	1848:1887	their physical and biological properties	1848:1887	Overall, this work demonstrates the utility of polyphosphonium-HA networks for the loading and slow release of ionic drugs and that their physical and biological properties can be readily tuned according to the structure of the phosphonium polymer.
31502452	3	66	theme	phosphonium	620:630	arg1	chloride					632:639	sodium hyaluronate (HA) and poly[tris(hydroxypropyl)(4-vinylbenzyl)phosphonium chloride]	553:640	chloride	632:639	Here we report the preparation and study of polyelectrolyte complexes (PECs) from sodium hyaluronate (HA) and poly[tris(hydroxypropyl)(4-vinylbenzyl)phosphonium chloride], poly[triphenyl(4-vinylbenzyl)phosphonium chloride], poly[tri(n-butyl)(4-vinylbenzyl)phosphonium chloride], or poly[triethyl(4-vinylbenzyl)phosphonium chloride].
31502452	1	67	used	used	223:226	arg2	complexation					109:120	Polyelectrolyte complexation	93:120	Polyelectrolyte complexation	93:120	Polyelectrolyte complexation, the combination of anionically and cationically charged polymers through ionic interactions, can be used to form hydrogel networks.
31502452	1	67	used	used	223:226	arg2	combination					127:137	the combination	123:137	the combination of anionically and cationically charged polymers through ionic interactions	123:213	Polyelectrolyte complexation, the combination of anionically and cationically charged polymers through ionic interactions, can be used to form hydrogel networks.
31502452	3	68	theme	phosphonium	781:791	arg1	chloride					793:800	poly[triethyl(4-vinylbenzyl)phosphonium chloride	753:800	poly[triethyl(4-vinylbenzyl)phosphonium chloride	753:800	Here we report the preparation and study of polyelectrolyte complexes (PECs) from sodium hyaluronate (HA) and poly[tris(hydroxypropyl)(4-vinylbenzyl)phosphonium chloride], poly[triphenyl(4-vinylbenzyl)phosphonium chloride], poly[tri(n-butyl)(4-vinylbenzyl)phosphonium chloride], or poly[triethyl(4-vinylbenzyl)phosphonium chloride].
31502452	3	69	theme	phosphonium	727:737	arg1	chloride					739:746	poly[tri(n-butyl)(4-vinylbenzyl)phosphonium chloride]	695:747	poly[tri(n-butyl)(4-vinylbenzyl)phosphonium chloride]	695:747	Here we report the preparation and study of polyelectrolyte complexes (PECs) from sodium hyaluronate (HA) and poly[tris(hydroxypropyl)(4-vinylbenzyl)phosphonium chloride], poly[triphenyl(4-vinylbenzyl)phosphonium chloride], poly[tri(n-butyl)(4-vinylbenzyl)phosphonium chloride], or poly[triethyl(4-vinylbenzyl)phosphonium chloride].
31502452	3	70	from	chloride	739:746	arg1	study					506:510	study	506:510	study	506:510	Here we report the preparation and study of polyelectrolyte complexes (PECs) from sodium hyaluronate (HA) and poly[tris(hydroxypropyl)(4-vinylbenzyl)phosphonium chloride], poly[triphenyl(4-vinylbenzyl)phosphonium chloride], poly[tri(n-butyl)(4-vinylbenzyl)phosphonium chloride], or poly[triethyl(4-vinylbenzyl)phosphonium chloride].
31502452	3	70	from	chloride	739:746	arg1	preparation					490:500	the preparation	486:500	the preparation	486:500	Here we report the preparation and study of polyelectrolyte complexes (PECs) from sodium hyaluronate (HA) and poly[tris(hydroxypropyl)(4-vinylbenzyl)phosphonium chloride], poly[triphenyl(4-vinylbenzyl)phosphonium chloride], poly[tri(n-butyl)(4-vinylbenzyl)phosphonium chloride], or poly[triethyl(4-vinylbenzyl)phosphonium chloride].
31502452	6	71	theme	PEC	1493:1495	arg1	network					1497:1503	the dense PEC network	1483:1503	the dense PEC network	1483:1503	Fluorescein and diclofenac were slowly released over 60 days, which was attributed to a combination of hydrophobic and ionic interactions with the dense PEC network.
31502452	1	72	theme	anionically	142:152	arg1	polymers					179:186	anionically and cationically charged polymers	142:186	anionically and cationically charged polymers	142:186	Polyelectrolyte complexation, the combination of anionically and cationically charged polymers through ionic interactions, can be used to form hydrogel networks.
31502452	1	73	theme	hydrogel	236:243	arg1	networks					245:252	hydrogel networks	236:252	hydrogel networks	236:252	Polyelectrolyte complexation, the combination of anionically and cationically charged polymers through ionic interactions, can be used to form hydrogel networks.
31502452	5	74	theme	loading	1303:1309	arg1	capacities					1311:1320	high loading capacities	1298:1320	high loading capacities of up to 16 wt %	1298:1337	Their properties depended on the structure of the phosphonium polymer and the salt concentration, but in general, they exhibited predominantly gel-like behavior with relaxation times greater than 40 s and self-healing over 2-18 h. Anionic molecules, including fluorescein, diclofenac, and adenosine-5'-triphosphate, were encapsulated into the PECs with high loading capacities of up to 16 wt %.
31502452	3	75	theme	4-vinylbenzyl	606:618	arg1	chloride					632:639	sodium hyaluronate (HA) and poly[tris(hydroxypropyl)(4-vinylbenzyl)phosphonium chloride]	553:640	chloride	632:639	Here we report the preparation and study of polyelectrolyte complexes (PECs) from sodium hyaluronate (HA) and poly[tris(hydroxypropyl)(4-vinylbenzyl)phosphonium chloride], poly[triphenyl(4-vinylbenzyl)phosphonium chloride], poly[tri(n-butyl)(4-vinylbenzyl)phosphonium chloride], or poly[triethyl(4-vinylbenzyl)phosphonium chloride].
31502452	7	76	theme	network	1707:1713	arg1	structure					1656:1664	the structure	1652:1664	the structure of the polymer	1652:1679	The cytotoxicities of the polymers and their corresponding networks with HA to C2C12 mouse myoblast cells was investigated and found to depend on the structure of the polymer and the properties of the network.
31502452	7	76	theme	network	1707:1713	arg1	properties					1689:1698	the properties	1685:1698	the properties of the network	1685:1713	The cytotoxicities of the polymers and their corresponding networks with HA to C2C12 mouse myoblast cells was investigated and found to depend on the structure of the polymer and the properties of the network.
31502452	5	77	theme	Anionic	1176:1182	arg1	diclofenac					1218:1227	diclofenac	1218:1227	diclofenac	1218:1227	Their properties depended on the structure of the phosphonium polymer and the salt concentration, but in general, they exhibited predominantly gel-like behavior with relaxation times greater than 40 s and self-healing over 2-18 h. Anionic molecules, including fluorescein, diclofenac, and adenosine-5'-triphosphate, were encapsulated into the PECs with high loading capacities of up to 16 wt %.
31502452	5	77	theme	Anionic	1176:1182	arg1	molecules					1184:1192	Anionic molecules	1176:1192	Anionic molecules	1176:1192	Their properties depended on the structure of the phosphonium polymer and the salt concentration, but in general, they exhibited predominantly gel-like behavior with relaxation times greater than 40 s and self-healing over 2-18 h. Anionic molecules, including fluorescein, diclofenac, and adenosine-5'-triphosphate, were encapsulated into the PECs with high loading capacities of up to 16 wt %.
31502452	5	77	theme	Anionic	1176:1182	arg1	fluorescein					1205:1215	fluorescein	1205:1215	fluorescein	1205:1215	Their properties depended on the structure of the phosphonium polymer and the salt concentration, but in general, they exhibited predominantly gel-like behavior with relaxation times greater than 40 s and self-healing over 2-18 h. Anionic molecules, including fluorescein, diclofenac, and adenosine-5'-triphosphate, were encapsulated into the PECs with high loading capacities of up to 16 wt %.
31502452	5	77	theme	Anionic	1176:1182	arg1	adenosine-5'-triphosphate					1234:1258	adenosine-5'-triphosphate	1234:1258	adenosine-5'-triphosphate	1234:1258	Their properties depended on the structure of the phosphonium polymer and the salt concentration, but in general, they exhibited predominantly gel-like behavior with relaxation times greater than 40 s and self-healing over 2-18 h. Anionic molecules, including fluorescein, diclofenac, and adenosine-5'-triphosphate, were encapsulated into the PECs with high loading capacities of up to 16 wt %.
31502452	3	78	theme	4-vinylbenzyl	767:779	arg1	chloride					793:800	poly[triethyl(4-vinylbenzyl)phosphonium chloride	753:800	poly[triethyl(4-vinylbenzyl)phosphonium chloride	753:800	Here we report the preparation and study of polyelectrolyte complexes (PECs) from sodium hyaluronate (HA) and poly[tris(hydroxypropyl)(4-vinylbenzyl)phosphonium chloride], poly[triphenyl(4-vinylbenzyl)phosphonium chloride], poly[tri(n-butyl)(4-vinylbenzyl)phosphonium chloride], or poly[triethyl(4-vinylbenzyl)phosphonium chloride].
31502452	3	79	theme	4-vinylbenzyl	713:725	arg1	chloride					739:746	poly[tri(n-butyl)(4-vinylbenzyl)phosphonium chloride]	695:747	poly[tri(n-butyl)(4-vinylbenzyl)phosphonium chloride]	695:747	Here we report the preparation and study of polyelectrolyte complexes (PECs) from sodium hyaluronate (HA) and poly[tris(hydroxypropyl)(4-vinylbenzyl)phosphonium chloride], poly[triphenyl(4-vinylbenzyl)phosphonium chloride], poly[tri(n-butyl)(4-vinylbenzyl)phosphonium chloride], or poly[triethyl(4-vinylbenzyl)phosphonium chloride].
31502452	4	80	theme	self-healing	907:918	arg1	properties					920:929	self-healing properties	907:929	self-healing properties	907:929	The networks were compacted by ultracentrifugation, then their composition, swelling, rheological, and self-healing properties were studied.
31502452	3	81	theme	poly[triphenyl	643:656	arg1	HA					573:574	sodium hyaluronate (HA) and poly[tris(hydroxypropyl)(4-vinylbenzyl)phosphonium chloride]	553:640	HA	573:574	Here we report the preparation and study of polyelectrolyte complexes (PECs) from sodium hyaluronate (HA) and poly[tris(hydroxypropyl)(4-vinylbenzyl)phosphonium chloride], poly[triphenyl(4-vinylbenzyl)phosphonium chloride], poly[tri(n-butyl)(4-vinylbenzyl)phosphonium chloride], or poly[triethyl(4-vinylbenzyl)phosphonium chloride].
31502452	3	81	theme	poly[triphenyl	643:656	arg1	chloride					684:691	poly[triphenyl(4-vinylbenzyl)phosphonium chloride]	643:692	poly[triphenyl(4-vinylbenzyl)phosphonium chloride]	643:692	Here we report the preparation and study of polyelectrolyte complexes (PECs) from sodium hyaluronate (HA) and poly[tris(hydroxypropyl)(4-vinylbenzyl)phosphonium chloride], poly[triphenyl(4-vinylbenzyl)phosphonium chloride], poly[tri(n-butyl)(4-vinylbenzyl)phosphonium chloride], or poly[triethyl(4-vinylbenzyl)phosphonium chloride].
31502452	5	82	theme	self-healing	1150:1161	arg1	times					1122:1126	relaxation times	1111:1126	relaxation times greater than 40 s and self-healing over 2-18 h. Anionic molecules, including fluorescein, diclofenac, and adenosine-5'-triphosphate, were encapsulated into the PECs with high loading capacities of up to 16 wt %	1111:1337	Their properties depended on the structure of the phosphonium polymer and the salt concentration, but in general, they exhibited predominantly gel-like behavior with relaxation times greater than 40 s and self-healing over 2-18 h. Anionic molecules, including fluorescein, diclofenac, and adenosine-5'-triphosphate, were encapsulated into the PECs with high loading capacities of up to 16 wt %.
31502452	2	83	used	used	277:280	arg2	networks					261:268	These networks	255:268	These networks	255:268	These networks can be used to encapsulate and release cargo, but the release of cargo is typically rapid, occurring over a period of hours to a few days and they often exhibit weak, fluid-like mechanical properties.
31531910	11	0	theme	ranking	2018:2024	arg1	animals					2026:2032	ranking animals	2018:2032	ranking animals	2018:2032	The accuracies of prediction are relatively low if the interest is in precisely predicting faecal nutrient digestibility of individual pigs, but are promising from the perspective of ranking animals in a genetic selection context.
31531910	7	1	theme	non-starch	1169:1178	arg1	polysaccharides					1180:1194	non-starch polysaccharides	1169:1194	non-starch polysaccharides	1169:1194	The microbiability values were significantly greater than zero for dry matter, organic matter, crude protein, crude fibre and non-starch polysaccharides, ranging from 0.58 to 0.93, as well as for crude fat with a value of 0.37, but not significantly different from zero for ash.
31531910	10	2	theme	fibre	1797:1801	arg1	digestibility					1731:1743	faecal digestibility	1724:1743	faecal digestibility of dry matter, organic matter, crude protein, crude fibre and non-starch polysaccharides	1724:1832	In conclusion, the faecal microbiota composition gave high microbiability values for faecal digestibility of dry matter, organic matter, crude protein, crude fibre and non-starch polysaccharides.
31531910	2	3	theme	nutrient	272:279	arg1	digestibility					281:293	faecal nutrient digestibility	265:293	faecal nutrient digestibility in grower-finisher pigs	265:317	This study aimed to use metagenomics to predict faecal nutrient digestibility in grower-finisher pigs.
31531910	5	4	theme	faecal	849:854	arg1	microbiome					856:865	the faecal microbiome	845:865	the faecal microbiome	845:865	The faecal samples were also sequenced for the 16S hypervariable region of bacteria (V3/V4) to profile the faecal microbiome.
31531910	11	5	theme	genetic	2039:2045	arg1	context					2057:2063	a genetic selection context	2037:2063	a genetic selection context	2037:2063	The accuracies of prediction are relatively low if the interest is in precisely predicting faecal nutrient digestibility of individual pigs, but are promising from the perspective of ranking animals in a genetic selection context.
31531910	4	6	theme	dry	639:641	arg1	matter					643:648	dry matter	639:648	dry matter	639:648	On the day before slaughter, faecal samples were collected and used to determine faecal digestibility of dry matter, ash, organic matter, crude protein, crude fat, crude fibre and non-starch polysaccharides.
31531910	5	7	theme	bacteria	817:824	arg1	region					807:812	the 16S hypervariable region	785:812	the 16S hypervariable region of bacteria (V3/V4) to profile the faecal microbiome	785:865	The faecal samples were also sequenced for the 16S hypervariable region of bacteria (V3/V4) to profile the faecal microbiome.
31531910	6	8	theme	between-animal	903:916	arg1	variation					918:926	the between-animal variation	899:926	the between-animal variation in faecal nutrient digestibility associated with variation in the faecal microbiome, that is the "microbiability"	899:1040	With these data, we calculated the between-animal variation in faecal nutrient digestibility associated with variation in the faecal microbiome, that is the "microbiability".
31531910	11	9	theme	prediction	1853:1862	arg1	promising					1984:1992	promising	1984:1992	promising	1984:1992	The accuracies of prediction are relatively low if the interest is in precisely predicting faecal nutrient digestibility of individual pigs, but are promising from the perspective of ranking animals in a genetic selection context.
31531910	11	9	theme	prediction	1853:1862	arg1	low					1879:1881	low	1879:1881	low	1879:1881	The accuracies of prediction are relatively low if the interest is in precisely predicting faecal nutrient digestibility of individual pigs, but are promising from the perspective of ranking animals in a genetic selection context.
31531910	11	9	theme	prediction	1853:1862	arg1	accuracies					1839:1848	The accuracies	1835:1848	The accuracies of prediction	1835:1862	The accuracies of prediction are relatively low if the interest is in precisely predicting faecal nutrient digestibility of individual pigs, but are promising from the perspective of ranking animals in a genetic selection context.
31531910	4	10	used	used	597:600	arg2	samples					570:576	faecal samples	563:576	faecal samples	563:576	On the day before slaughter, faecal samples were collected and used to determine faecal digestibility of dry matter, ash, organic matter, crude protein, crude fat, crude fibre and non-starch polysaccharides.
31531910	10	11	theme	faecal	1658:1663	arg1	composition					1676:1686	the faecal microbiota composition	1654:1686	the faecal microbiota composition	1654:1686	In conclusion, the faecal microbiota composition gave high microbiability values for faecal digestibility of dry matter, organic matter, crude protein, crude fibre and non-starch polysaccharides.
31531910	5	12	theme	16S	789:791	arg1	region					807:812	the 16S hypervariable region	785:812	the 16S hypervariable region of bacteria (V3/V4) to profile the faecal microbiome	785:865	The faecal samples were also sequenced for the 16S hypervariable region of bacteria (V3/V4) to profile the faecal microbiome.
31531910	11	13	theme	individual	1959:1968	arg1	pigs					1970:1973	individual pigs	1959:1973	individual pigs	1959:1973	The accuracies of prediction are relatively low if the interest is in precisely predicting faecal nutrient digestibility of individual pigs, but are promising from the perspective of ranking animals in a genetic selection context.
31531910	11	14	from	perspective	2003:2013	arg1	promising					1984:1992	promising	1984:1992	promising	1984:1992	The accuracies of prediction are relatively low if the interest is in precisely predicting faecal nutrient digestibility of individual pigs, but are promising from the perspective of ranking animals in a genetic selection context.
31531910	11	14	from	perspective	2003:2013	arg1	context					2057:2063	a genetic selection context	2037:2063	a genetic selection context	2037:2063	The accuracies of prediction are relatively low if the interest is in precisely predicting faecal nutrient digestibility of individual pigs, but are promising from the perspective of ranking animals in a genetic selection context.
31531910	11	14	from	perspective	2003:2013	arg1	low					1879:1881	low	1879:1881	low	1879:1881	The accuracies of prediction are relatively low if the interest is in precisely predicting faecal nutrient digestibility of individual pigs, but are promising from the perspective of ranking animals in a genetic selection context.
31531910	11	14	from	perspective	2003:2013	arg1	accuracies					1839:1848	The accuracies	1835:1848	The accuracies of prediction	1835:1862	The accuracies of prediction are relatively low if the interest is in precisely predicting faecal nutrient digestibility of individual pigs, but are promising from the perspective of ranking animals in a genetic selection context.
31531910	7	15	theme	dry	1110:1112	arg1	matter					1114:1119	dry matter	1110:1119	dry matter	1110:1119	The microbiability values were significantly greater than zero for dry matter, organic matter, crude protein, crude fibre and non-starch polysaccharides, ranging from 0.58 to 0.93, as well as for crude fat with a value of 0.37, but not significantly different from zero for ash.
31531910	4	16	theme	crude	672:676	arg1	protein					678:684	crude protein	672:684	crude protein	672:684	On the day before slaughter, faecal samples were collected and used to determine faecal digestibility of dry matter, ash, organic matter, crude protein, crude fat, crude fibre and non-starch polysaccharides.
31531910	10	17	theme	high	1693:1696	arg1	values					1713:1718	high microbiability values	1693:1718	high microbiability values for faecal digestibility of dry matter, organic matter, crude protein, crude fibre and non-starch polysaccharides	1693:1832	In conclusion, the faecal microbiota composition gave high microbiability values for faecal digestibility of dry matter, organic matter, crude protein, crude fibre and non-starch polysaccharides.
31531910	1	18	theme	total	138:142	arg1	digestion					159:167	total tract nutrient digestion	138:167	total tract nutrient digestion	138:167	Microbiota play an important role in total tract nutrient digestion, especially when fibrous diets are fed to pigs.
31531910	7	19	from	zero	1308:1311	arg1	different					1293:1301	different	1293:1301	different	1293:1301	The microbiability values were significantly greater than zero for dry matter, organic matter, crude protein, crude fibre and non-starch polysaccharides, ranging from 0.58 to 0.93, as well as for crude fat with a value of 0.37, but not significantly different from zero for ash.
31531910	10	20	theme	faecal	1724:1729	arg1	digestibility					1731:1743	faecal digestibility	1724:1743	faecal digestibility of dry matter, organic matter, crude protein, crude fibre and non-starch polysaccharides	1724:1832	In conclusion, the faecal microbiota composition gave high microbiability values for faecal digestibility of dry matter, organic matter, crude protein, crude fibre and non-starch polysaccharides.
31531910	1	21	theme	nutrient	150:157	arg1	digestion					159:167	total tract nutrient digestion	138:167	total tract nutrient digestion	138:167	Microbiota play an important role in total tract nutrient digestion, especially when fibrous diets are fed to pigs.
31531910	8	22	theme	digestibility	1400:1412	arg1	values					1414:1419	digestibility values	1400:1419	digestibility values of individual pigs based on their faecal microbiota composition	1400:1483	Using leave-one-out cross-validation, we estimated the accuracy of predicting digestibility values of individual pigs based on their faecal microbiota composition.
31531910	4	23	theme	matter	664:669	arg1	digestibility					622:634	faecal digestibility	615:634	faecal digestibility of dry matter, ash, organic matter, crude protein, crude fat, crude fibre and non-starch polysaccharides	615:739	On the day before slaughter, faecal samples were collected and used to determine faecal digestibility of dry matter, ash, organic matter, crude protein, crude fat, crude fibre and non-starch polysaccharides.
31531910	0	24	theme	microbiota	77:86	arg1	composition					88:98	faecal microbiota composition	70:98	faecal microbiota composition	70:98	Prediction of nutrient digestibility in grower-finisher pigs based on faecal microbiota composition.
31531910	6	25	theme	nutrient	938:945	arg1	digestibility					947:959	faecal nutrient digestibility	931:959	faecal nutrient digestibility associated with variation in the faecal microbiome, that is the "microbiability"	931:1040	With these data, we calculated the between-animal variation in faecal nutrient digestibility associated with variation in the faecal microbiome, that is the "microbiability".
31531910	3	26	theme	three-way	354:362	arg1	pigs					391:394	160 three-way crossbreed grower-finisher pigs	350:394	160 three-way crossbreed grower-finisher pigs (80 female and 80 male) which were either fed a diet based on corn/soybean meal or a more fibrous diet based on wheat/barley/by-products	350:531	The study design consisted of 160 three-way crossbreed grower-finisher pigs (80 female and 80 male) which were either fed a diet based on corn/soybean meal or a more fibrous diet based on wheat/barley/by-products.
31531910	3	26	theme	three-way	354:362	arg1	female					400:405	80 female	397:405	80 female	397:405	The study design consisted of 160 three-way crossbreed grower-finisher pigs (80 female and 80 male) which were either fed a diet based on corn/soybean meal or a more fibrous diet based on wheat/barley/by-products.
31531910	3	26	theme	three-way	354:362	arg1	male					414:417	80 male	411:417	80 male	411:417	The study design consisted of 160 three-way crossbreed grower-finisher pigs (80 female and 80 male) which were either fed a diet based on corn/soybean meal or a more fibrous diet based on wheat/barley/by-products.
31531910	0	27	from	Prediction	0:9	arg1	pigs					56:59	grower-finisher pigs	40:59	grower-finisher pigs	40:59	Prediction of nutrient digestibility in grower-finisher pigs based on faecal microbiota composition.
31531910	8	28	theme	pigs	1435:1438	arg1	values					1414:1419	digestibility values	1400:1419	digestibility values of individual pigs based on their faecal microbiota composition	1400:1483	Using leave-one-out cross-validation, we estimated the accuracy of predicting digestibility values of individual pigs based on their faecal microbiota composition.
31531910	4	29	theme	polysaccharides	725:739	arg1	digestibility					622:634	faecal digestibility	615:634	faecal digestibility of dry matter, ash, organic matter, crude protein, crude fat, crude fibre and non-starch polysaccharides	615:739	On the day before slaughter, faecal samples were collected and used to determine faecal digestibility of dry matter, ash, organic matter, crude protein, crude fat, crude fibre and non-starch polysaccharides.
31531910	2	30	from	digestibility	281:293	arg1	pigs					314:317	grower-finisher pigs	298:317	grower-finisher pigs	298:317	This study aimed to use metagenomics to predict faecal nutrient digestibility in grower-finisher pigs.
31531910	7	31	dep	0.93	1218:1221	arg1	to					1215:1216	to	1215:1216	to	1215:1216	The microbiability values were significantly greater than zero for dry matter, organic matter, crude protein, crude fibre and non-starch polysaccharides, ranging from 0.58 to 0.93, as well as for crude fat with a value of 0.37, but not significantly different from zero for ash.
31531910	4	32	theme	fat	693:695	arg1	digestibility					622:634	faecal digestibility	615:634	faecal digestibility of dry matter, ash, organic matter, crude protein, crude fat, crude fibre and non-starch polysaccharides	615:739	On the day before slaughter, faecal samples were collected and used to determine faecal digestibility of dry matter, ash, organic matter, crude protein, crude fat, crude fibre and non-starch polysaccharides.
31531910	7	33	theme	organic	1122:1128	arg1	matter					1130:1135	organic matter	1122:1135	organic matter	1122:1135	The microbiability values were significantly greater than zero for dry matter, organic matter, crude protein, crude fibre and non-starch polysaccharides, ranging from 0.58 to 0.93, as well as for crude fat with a value of 0.37, but not significantly different from zero for ash.
31531910	7	34	theme	crude	1239:1243	arg1	fat					1245:1247	crude fat	1239:1247	crude fat with a value of 0.37	1239:1268	The microbiability values were significantly greater than zero for dry matter, organic matter, crude protein, crude fibre and non-starch polysaccharides, ranging from 0.58 to 0.93, as well as for crude fat with a value of 0.37, but not significantly different from zero for ash.
31531910	3	35	theme	grower-finisher	375:389	arg1	pigs					391:394	160 three-way crossbreed grower-finisher pigs	350:394	160 three-way crossbreed grower-finisher pigs (80 female and 80 male) which were either fed a diet based on corn/soybean meal or a more fibrous diet based on wheat/barley/by-products	350:531	The study design consisted of 160 three-way crossbreed grower-finisher pigs (80 female and 80 male) which were either fed a diet based on corn/soybean meal or a more fibrous diet based on wheat/barley/by-products.
31531910	3	35	theme	grower-finisher	375:389	arg1	female					400:405	80 female	397:405	80 female	397:405	The study design consisted of 160 three-way crossbreed grower-finisher pigs (80 female and 80 male) which were either fed a diet based on corn/soybean meal or a more fibrous diet based on wheat/barley/by-products.
31531910	3	35	theme	grower-finisher	375:389	arg1	male					414:417	80 male	411:417	80 male	411:417	The study design consisted of 160 three-way crossbreed grower-finisher pigs (80 female and 80 male) which were either fed a diet based on corn/soybean meal or a more fibrous diet based on wheat/barley/by-products.
31531910	8	36	theme	faecal	1455:1460	arg1	composition					1473:1483	their faecal microbiota composition	1449:1483	their faecal microbiota composition	1449:1483	Using leave-one-out cross-validation, we estimated the accuracy of predicting digestibility values of individual pigs based on their faecal microbiota composition.
31531910	0	37	theme	nutrient	14:21	arg1	digestibility					23:35	nutrient digestibility	14:35	nutrient digestibility	14:35	Prediction of nutrient digestibility in grower-finisher pigs based on faecal microbiota composition.
31531910	9	38	theme	prediction	1504:1513	arg1	accuracies					1490:1499	The accuracies	1486:1499	The accuracies of prediction for crude fat and ash digestibility	1486:1549	The accuracies of prediction for crude fat and ash digestibility were virtually 0, and for the other nutrients, the accuracies ranged from 0.42 to 0.63.
31531910	9	38	theme	prediction	1504:1513	arg1	0					1566:1566	0	1566:1566	0	1566:1566	The accuracies of prediction for crude fat and ash digestibility were virtually 0, and for the other nutrients, the accuracies ranged from 0.42 to 0.63.
31531910	6	39	from	variation	918:926	arg1	digestibility					947:959	faecal nutrient digestibility	931:959	faecal nutrient digestibility associated with variation in the faecal microbiome, that is the "microbiability"	931:1040	With these data, we calculated the between-animal variation in faecal nutrient digestibility associated with variation in the faecal microbiome, that is the "microbiability".
31531910	10	40	theme	organic	1760:1766	arg1	matter					1768:1773	organic matter	1760:1773	organic matter	1760:1773	In conclusion, the faecal microbiota composition gave high microbiability values for faecal digestibility of dry matter, organic matter, crude protein, crude fibre and non-starch polysaccharides.
31531910	9	41	theme	crude	1519:1523	arg1	fat					1525:1527	crude fat	1519:1527	crude fat	1519:1527	The accuracies of prediction for crude fat and ash digestibility were virtually 0, and for the other nutrients, the accuracies ranged from 0.42 to 0.63.
31531910	10	42	theme	matter	1752:1757	arg1	digestibility					1731:1743	faecal digestibility	1724:1743	faecal digestibility of dry matter, organic matter, crude protein, crude fibre and non-starch polysaccharides	1724:1832	In conclusion, the faecal microbiota composition gave high microbiability values for faecal digestibility of dry matter, organic matter, crude protein, crude fibre and non-starch polysaccharides.
31531910	11	43	theme	selection	2047:2055	arg1	context					2057:2063	a genetic selection context	2037:2063	a genetic selection context	2037:2063	The accuracies of prediction are relatively low if the interest is in precisely predicting faecal nutrient digestibility of individual pigs, but are promising from the perspective of ranking animals in a genetic selection context.
31531910	10	44	theme	protein	1782:1788	arg1	digestibility					1731:1743	faecal digestibility	1724:1743	faecal digestibility of dry matter, organic matter, crude protein, crude fibre and non-starch polysaccharides	1724:1832	In conclusion, the faecal microbiota composition gave high microbiability values for faecal digestibility of dry matter, organic matter, crude protein, crude fibre and non-starch polysaccharides.
31531910	8	45	theme	leave-one-out	1328:1340	arg1	cross-validation					1342:1357	leave-one-out cross-validation	1328:1357	leave-one-out cross-validation	1328:1357	Using leave-one-out cross-validation, we estimated the accuracy of predicting digestibility values of individual pigs based on their faecal microbiota composition.
31531910	3	46	theme	fibrous	486:492	arg1	diet					494:497	a more fibrous diet	479:497	a more fibrous diet based on wheat/barley/by-products	479:531	The study design consisted of 160 three-way crossbreed grower-finisher pigs (80 female and 80 male) which were either fed a diet based on corn/soybean meal or a more fibrous diet based on wheat/barley/by-products.
31531910	11	47	theme	animals	2026:2032	arg1	perspective					2003:2013	the perspective	1999:2013	the perspective of ranking animals in a genetic selection context	1999:2063	The accuracies of prediction are relatively low if the interest is in precisely predicting faecal nutrient digestibility of individual pigs, but are promising from the perspective of ranking animals in a genetic selection context.
31531910	6	48	theme	microbiability	1026:1039	arg1	microbiome					1001:1010	the faecal microbiome	990:1010	the faecal microbiome	990:1010	With these data, we calculated the between-animal variation in faecal nutrient digestibility associated with variation in the faecal microbiome, that is the "microbiability".
31531910	6	48	theme	microbiability	1026:1039	arg1	"					1040:1040	the "microbiability"	1021:1040	the "microbiability"	1021:1040	With these data, we calculated the between-animal variation in faecal nutrient digestibility associated with variation in the faecal microbiome, that is the "microbiability".
31531910	7	49	theme	microbiability	1047:1060	arg1	values					1062:1067	The microbiability values	1043:1067	The microbiability values	1043:1067	The microbiability values were significantly greater than zero for dry matter, organic matter, crude protein, crude fibre and non-starch polysaccharides, ranging from 0.58 to 0.93, as well as for crude fat with a value of 0.37, but not significantly different from zero for ash.
31531910	7	49	theme	microbiability	1047:1060	arg1	greater					1088:1094	greater	1088:1094	greater	1088:1094	The microbiability values were significantly greater than zero for dry matter, organic matter, crude protein, crude fibre and non-starch polysaccharides, ranging from 0.58 to 0.93, as well as for crude fat with a value of 0.37, but not significantly different from zero for ash.
31531910	4	50	theme	faecal	563:568	arg1	samples					570:576	faecal samples	563:576	faecal samples	563:576	On the day before slaughter, faecal samples were collected and used to determine faecal digestibility of dry matter, ash, organic matter, crude protein, crude fat, crude fibre and non-starch polysaccharides.
31531910	2	51	theme	faecal	265:270	arg1	digestibility					281:293	faecal nutrient digestibility	265:293	faecal nutrient digestibility in grower-finisher pigs	265:317	This study aimed to use metagenomics to predict faecal nutrient digestibility in grower-finisher pigs.
31531910	9	52	theme	other	1581:1585	arg1	nutrients					1587:1595	the other nutrients	1577:1595	the other nutrients	1577:1595	The accuracies of prediction for crude fat and ash digestibility were virtually 0, and for the other nutrients, the accuracies ranged from 0.42 to 0.63.
31531910	10	53	theme	polysaccharides	1818:1832	arg1	digestibility					1731:1743	faecal digestibility	1724:1743	faecal digestibility of dry matter, organic matter, crude protein, crude fibre and non-starch polysaccharides	1724:1832	In conclusion, the faecal microbiota composition gave high microbiability values for faecal digestibility of dry matter, organic matter, crude protein, crude fibre and non-starch polysaccharides.
31531910	1	54	theme	fibrous	186:192	arg1	diets					194:198	fibrous diets	186:198	fibrous diets	186:198	Microbiota play an important role in total tract nutrient digestion, especially when fibrous diets are fed to pigs.
31531910	4	55	theme	matter	643:648	arg1	digestibility					622:634	faecal digestibility	615:634	faecal digestibility of dry matter, ash, organic matter, crude protein, crude fat, crude fibre and non-starch polysaccharides	615:739	On the day before slaughter, faecal samples were collected and used to determine faecal digestibility of dry matter, ash, organic matter, crude protein, crude fat, crude fibre and non-starch polysaccharides.
31531910	2	56	theme	grower-finisher	298:312	arg1	pigs					314:317	grower-finisher pigs	298:317	grower-finisher pigs	298:317	This study aimed to use metagenomics to predict faecal nutrient digestibility in grower-finisher pigs.
31531910	5	57	theme	hypervariable	793:805	arg1	region					807:812	the 16S hypervariable region	785:812	the 16S hypervariable region of bacteria (V3/V4) to profile the faecal microbiome	785:865	The faecal samples were also sequenced for the 16S hypervariable region of bacteria (V3/V4) to profile the faecal microbiome.
31531910	11	58	theme	pigs	1970:1973	arg1	digestibility					1942:1954	faecal nutrient digestibility	1926:1954	faecal nutrient digestibility of individual pigs	1926:1973	The accuracies of prediction are relatively low if the interest is in precisely predicting faecal nutrient digestibility of individual pigs, but are promising from the perspective of ranking animals in a genetic selection context.
31531910	4	59	theme	faecal	615:620	arg1	digestibility					622:634	faecal digestibility	615:634	faecal digestibility of dry matter, ash, organic matter, crude protein, crude fat, crude fibre and non-starch polysaccharides	615:739	On the day before slaughter, faecal samples were collected and used to determine faecal digestibility of dry matter, ash, organic matter, crude protein, crude fat, crude fibre and non-starch polysaccharides.
31531910	9	60	dep	0.63	1633:1636	arg1	to					1630:1631	to	1630:1631	to	1630:1631	The accuracies of prediction for crude fat and ash digestibility were virtually 0, and for the other nutrients, the accuracies ranged from 0.42 to 0.63.
31531910	10	61	theme	microbiability	1698:1711	arg1	values					1713:1718	high microbiability values	1693:1718	high microbiability values for faecal digestibility of dry matter, organic matter, crude protein, crude fibre and non-starch polysaccharides	1693:1832	In conclusion, the faecal microbiota composition gave high microbiability values for faecal digestibility of dry matter, organic matter, crude protein, crude fibre and non-starch polysaccharides.
31531910	1	62	theme	important	120:128	arg1	role					130:133	an important role	117:133	an important role	117:133	Microbiota play an important role in total tract nutrient digestion, especially when fibrous diets are fed to pigs.
31531910	7	63	with	fat	1245:1247	arg1	value					1256:1260	a value	1254:1260	a value of 0.37	1254:1268	The microbiability values were significantly greater than zero for dry matter, organic matter, crude protein, crude fibre and non-starch polysaccharides, ranging from 0.58 to 0.93, as well as for crude fat with a value of 0.37, but not significantly different from zero for ash.
31531910	10	64	theme	dry	1748:1750	arg1	matter					1752:1757	dry matter	1748:1757	dry matter	1748:1757	In conclusion, the faecal microbiota composition gave high microbiability values for faecal digestibility of dry matter, organic matter, crude protein, crude fibre and non-starch polysaccharides.
31531910	10	65	theme	microbiota	1665:1674	arg1	composition					1676:1686	the faecal microbiota composition	1654:1686	the faecal microbiota composition	1654:1686	In conclusion, the faecal microbiota composition gave high microbiability values for faecal digestibility of dry matter, organic matter, crude protein, crude fibre and non-starch polysaccharides.
31531910	7	66	theme	0.37	1265:1268	arg1	value					1256:1260	a value	1254:1260	a value of 0.37	1254:1268	The microbiability values were significantly greater than zero for dry matter, organic matter, crude protein, crude fibre and non-starch polysaccharides, ranging from 0.58 to 0.93, as well as for crude fat with a value of 0.37, but not significantly different from zero for ash.
31531910	4	67	theme	protein	678:684	arg1	digestibility					622:634	faecal digestibility	615:634	faecal digestibility of dry matter, ash, organic matter, crude protein, crude fat, crude fibre and non-starch polysaccharides	615:739	On the day before slaughter, faecal samples were collected and used to determine faecal digestibility of dry matter, ash, organic matter, crude protein, crude fat, crude fibre and non-starch polysaccharides.
31531910	0	68	theme	faecal	70:75	arg1	composition					88:98	faecal microbiota composition	70:98	faecal microbiota composition	70:98	Prediction of nutrient digestibility in grower-finisher pigs based on faecal microbiota composition.
31531910	1	69	theme	tract	144:148	arg1	digestion					159:167	total tract nutrient digestion	138:167	total tract nutrient digestion	138:167	Microbiota play an important role in total tract nutrient digestion, especially when fibrous diets are fed to pigs.
31531910	4	70	theme	ash	651:653	arg1	digestibility					622:634	faecal digestibility	615:634	faecal digestibility of dry matter, ash, organic matter, crude protein, crude fat, crude fibre and non-starch polysaccharides	615:739	On the day before slaughter, faecal samples were collected and used to determine faecal digestibility of dry matter, ash, organic matter, crude protein, crude fat, crude fibre and non-starch polysaccharides.
31531910	3	71	theme	study	324:328	arg1	design					330:335	The study design	320:335	The study design	320:335	The study design consisted of 160 three-way crossbreed grower-finisher pigs (80 female and 80 male) which were either fed a diet based on corn/soybean meal or a more fibrous diet based on wheat/barley/by-products.
31531910	8	72	theme	individual	1424:1433	arg1	pigs					1435:1438	individual pigs	1424:1438	individual pigs based on their faecal microbiota composition	1424:1483	Using leave-one-out cross-validation, we estimated the accuracy of predicting digestibility values of individual pigs based on their faecal microbiota composition.
31531910	3	73	dep	pigs	391:394	arg1	pigs					391:394	160 three-way crossbreed grower-finisher pigs	350:394	160 three-way crossbreed grower-finisher pigs (80 female and 80 male) which were either fed a diet based on corn/soybean meal or a more fibrous diet based on wheat/barley/by-products	350:531	The study design consisted of 160 three-way crossbreed grower-finisher pigs (80 female and 80 male) which were either fed a diet based on corn/soybean meal or a more fibrous diet based on wheat/barley/by-products.
31531910	3	73	dep	pigs	391:394	arg1	female					400:405	80 female	397:405	80 female	397:405	The study design consisted of 160 three-way crossbreed grower-finisher pigs (80 female and 80 male) which were either fed a diet based on corn/soybean meal or a more fibrous diet based on wheat/barley/by-products.
31531910	3	73	dep	pigs	391:394	arg1	male					414:417	80 male	411:417	80 male	411:417	The study design consisted of 160 three-way crossbreed grower-finisher pigs (80 female and 80 male) which were either fed a diet based on corn/soybean meal or a more fibrous diet based on wheat/barley/by-products.
31531910	4	74	theme	organic	656:662	arg1	matter					664:669	organic matter	656:669	organic matter	656:669	On the day before slaughter, faecal samples were collected and used to determine faecal digestibility of dry matter, ash, organic matter, crude protein, crude fat, crude fibre and non-starch polysaccharides.
31531910	7	75	theme	crude	1138:1142	arg1	protein					1144:1150	crude protein	1138:1150	crude protein	1138:1150	The microbiability values were significantly greater than zero for dry matter, organic matter, crude protein, crude fibre and non-starch polysaccharides, ranging from 0.58 to 0.93, as well as for crude fat with a value of 0.37, but not significantly different from zero for ash.
31531910	11	76	theme	nutrient	1933:1940	arg1	digestibility					1942:1954	faecal nutrient digestibility	1926:1954	faecal nutrient digestibility of individual pigs	1926:1973	The accuracies of prediction are relatively low if the interest is in precisely predicting faecal nutrient digestibility of individual pigs, but are promising from the perspective of ranking animals in a genetic selection context.
31531910	6	77	theme	faecal	931:936	arg1	digestibility					947:959	faecal nutrient digestibility	931:959	faecal nutrient digestibility associated with variation in the faecal microbiome, that is the "microbiability"	931:1040	With these data, we calculated the between-animal variation in faecal nutrient digestibility associated with variation in the faecal microbiome, that is the "microbiability".
31531910	3	78	theme	crossbreed	364:373	arg1	pigs					391:394	160 three-way crossbreed grower-finisher pigs	350:394	160 three-way crossbreed grower-finisher pigs (80 female and 80 male) which were either fed a diet based on corn/soybean meal or a more fibrous diet based on wheat/barley/by-products	350:531	The study design consisted of 160 three-way crossbreed grower-finisher pigs (80 female and 80 male) which were either fed a diet based on corn/soybean meal or a more fibrous diet based on wheat/barley/by-products.
31531910	3	78	theme	crossbreed	364:373	arg1	female					400:405	80 female	397:405	80 female	397:405	The study design consisted of 160 three-way crossbreed grower-finisher pigs (80 female and 80 male) which were either fed a diet based on corn/soybean meal or a more fibrous diet based on wheat/barley/by-products.
31531910	3	78	theme	crossbreed	364:373	arg1	male					414:417	80 male	411:417	80 male	411:417	The study design consisted of 160 three-way crossbreed grower-finisher pigs (80 female and 80 male) which were either fed a diet based on corn/soybean meal or a more fibrous diet based on wheat/barley/by-products.
31531910	6	79	from	variation	977:985	arg1	microbiome					1001:1010	the faecal microbiome	990:1010	the faecal microbiome	990:1010	With these data, we calculated the between-animal variation in faecal nutrient digestibility associated with variation in the faecal microbiome, that is the "microbiability".
31531910	6	79	from	variation	977:985	arg1	"					1040:1040	the "microbiability"	1021:1040	the "microbiability"	1021:1040	With these data, we calculated the between-animal variation in faecal nutrient digestibility associated with variation in the faecal microbiome, that is the "microbiability".
31531910	4	80	theme	fibre	704:708	arg1	digestibility					622:634	faecal digestibility	615:634	faecal digestibility of dry matter, ash, organic matter, crude protein, crude fat, crude fibre and non-starch polysaccharides	615:739	On the day before slaughter, faecal samples were collected and used to determine faecal digestibility of dry matter, ash, organic matter, crude protein, crude fat, crude fibre and non-starch polysaccharides.
31531910	4	81	theme	non-starch	714:723	arg1	polysaccharides					725:739	non-starch polysaccharides	714:739	non-starch polysaccharides	714:739	On the day before slaughter, faecal samples were collected and used to determine faecal digestibility of dry matter, ash, organic matter, crude protein, crude fat, crude fibre and non-starch polysaccharides.
31531910	8	82	theme	microbiota	1462:1471	arg1	composition					1473:1483	their faecal microbiota composition	1449:1483	their faecal microbiota composition	1449:1483	Using leave-one-out cross-validation, we estimated the accuracy of predicting digestibility values of individual pigs based on their faecal microbiota composition.
31531910	4	83	theme	crude	687:691	arg1	fat					693:695	crude fat	687:695	crude fat	687:695	On the day before slaughter, faecal samples were collected and used to determine faecal digestibility of dry matter, ash, organic matter, crude protein, crude fat, crude fibre and non-starch polysaccharides.
31531910	11	84	theme	faecal	1926:1931	arg1	digestibility					1942:1954	faecal nutrient digestibility	1926:1954	faecal nutrient digestibility of individual pigs	1926:1973	The accuracies of prediction are relatively low if the interest is in precisely predicting faecal nutrient digestibility of individual pigs, but are promising from the perspective of ranking animals in a genetic selection context.
31531910	0	85	theme	digestibility	23:35	arg1	Prediction					0:9	Prediction	0:9	Prediction of nutrient digestibility in grower-finisher pigs	0:59	Prediction of nutrient digestibility in grower-finisher pigs based on faecal microbiota composition.
31531910	10	86	theme	matter	1768:1773	arg1	digestibility					1731:1743	faecal digestibility	1724:1743	faecal digestibility of dry matter, organic matter, crude protein, crude fibre and non-starch polysaccharides	1724:1832	In conclusion, the faecal microbiota composition gave high microbiability values for faecal digestibility of dry matter, organic matter, crude protein, crude fibre and non-starch polysaccharides.
31531910	0	87	theme	grower-finisher	40:54	arg1	pigs					56:59	grower-finisher pigs	40:59	grower-finisher pigs	40:59	Prediction of nutrient digestibility in grower-finisher pigs based on faecal microbiota composition.
31531910	6	88	theme	faecal	994:999	arg1	microbiome					1001:1010	the faecal microbiome	990:1010	the faecal microbiome	990:1010	With these data, we calculated the between-animal variation in faecal nutrient digestibility associated with variation in the faecal microbiome, that is the "microbiability".
31531910	6	88	theme	faecal	994:999	arg1	"					1040:1040	the "microbiability"	1021:1040	the "microbiability"	1021:1040	With these data, we calculated the between-animal variation in faecal nutrient digestibility associated with variation in the faecal microbiome, that is the "microbiability".
31531910	5	89	theme	faecal	746:751	arg1	samples					753:759	The faecal samples	742:759	The faecal samples	742:759	The faecal samples were also sequenced for the 16S hypervariable region of bacteria (V3/V4) to profile the faecal microbiome.
31531910	9	90	theme	ash	1533:1535	arg1	digestibility					1537:1549	ash digestibility	1533:1549	ash digestibility	1533:1549	The accuracies of prediction for crude fat and ash digestibility were virtually 0, and for the other nutrients, the accuracies ranged from 0.42 to 0.63.
31531910	10	91	theme	crude	1776:1780	arg1	protein					1782:1788	crude protein	1776:1788	crude protein	1776:1788	In conclusion, the faecal microbiota composition gave high microbiability values for faecal digestibility of dry matter, organic matter, crude protein, crude fibre and non-starch polysaccharides.
31531910	3	92	theme	corn/soybean	458:469	arg1	meal					471:474	corn/soybean meal	458:474	corn/soybean meal	458:474	The study design consisted of 160 three-way crossbreed grower-finisher pigs (80 female and 80 male) which were either fed a diet based on corn/soybean meal or a more fibrous diet based on wheat/barley/by-products.
31531910	10	93	theme	non-starch	1807:1816	arg1	polysaccharides					1818:1832	non-starch polysaccharides	1807:1832	non-starch polysaccharides	1807:1832	In conclusion, the faecal microbiota composition gave high microbiability values for faecal digestibility of dry matter, organic matter, crude protein, crude fibre and non-starch polysaccharides.
30326910	2	0	theme	different	338:346	arg1	growths					364:370	different fungal mycelial growths	338:370	different fungal mycelial growths	338:370	Both articles also appear to use the same image representing different fungal mycelial growths.
30326910	0	1	theme	antimicrobial	68:80	arg1	activities					82:91	antimicrobial activities	68:91	antimicrobial activities	68:91	Retraction Note: Optimized extraction, composition, antioxidant and antimicrobial activities of exo and intracellular polysaccharides from submerged culture of Cordyceps cicadae.
30326910	4	2	theme	N.	432:433	arg1	Gautam					435:440	N. Gautam	432:440	N. Gautam	432:440	N. Gautam has not responded to correspondence from the editor about this retraction.
30326910	1	3	dep	overlap	228:234	arg1	due					221:223	due	221:223	due	221:223	The editor has retracted this article [1] due to overlap with a previously published article [2].
30326910	0	4	theme	exo	96:98	arg1	polysaccharides					118:132	exo and intracellular polysaccharides	96:132	exo and intracellular polysaccharides from submerged culture of Cordyceps cicadae	96:176	Retraction Note: Optimized extraction, composition, antioxidant and antimicrobial activities of exo and intracellular polysaccharides from submerged culture of Cordyceps cicadae.
30326910	1	5	theme	published	254:262	arg1	[2					272:273	a previously published article [2	241:273	a previously published article [2	241:273	The editor has retracted this article [1] due to overlap with a previously published article [2].
30326910	1	6	theme	article	264:270	arg1	[2					272:273	a previously published article [2	241:273	a previously published article [2	241:273	The editor has retracted this article [1] due to overlap with a previously published article [2].
30326910	0	7	theme	intracellular	104:116	arg1	polysaccharides					118:132	exo and intracellular polysaccharides	96:132	exo and intracellular polysaccharides from submerged culture of Cordyceps cicadae	96:176	Retraction Note: Optimized extraction, composition, antioxidant and antimicrobial activities of exo and intracellular polysaccharides from submerged culture of Cordyceps cicadae.
30326910	0	8	from	composition	39:49	arg1	culture					149:155	submerged culture	139:155	submerged culture of Cordyceps cicadae	139:176	Retraction Note: Optimized extraction, composition, antioxidant and antimicrobial activities of exo and intracellular polysaccharides from submerged culture of Cordyceps cicadae.
30326910	3	9	theme	S.K.	373:376	arg1	Sharma					378:383	S.K. Sharma	373:383	S.K. Sharma	373:383	S.K. Sharma and N.S. Atri do not agree to this retraction.
30326910	0	10	from	extraction	27:36	arg1	culture					149:155	submerged culture	139:155	submerged culture of Cordyceps cicadae	139:176	Retraction Note: Optimized extraction, composition, antioxidant and antimicrobial activities of exo and intracellular polysaccharides from submerged culture of Cordyceps cicadae.
30326910	0	11	theme	Retraction	0:9	arg1	Note					11:14	Retraction Note	0:14	Retraction Note: Optimized extraction, composition, antioxidant and antimicrobial activities of exo and intracellular polysaccharides from submerged culture of Cordyceps cicadae.	0:177	Retraction Note: Optimized extraction, composition, antioxidant and antimicrobial activities of exo and intracellular polysaccharides from submerged culture of Cordyceps cicadae.
30326910	0	12	theme	Optimized	17:25	arg1	extraction					27:36	extraction	27:36	extraction	27:36	Retraction Note: Optimized extraction, composition, antioxidant and antimicrobial activities of exo and intracellular polysaccharides from submerged culture of Cordyceps cicadae.
30326910	0	13	theme	polysaccharides	118:132	arg1	antioxidant					52:62	antioxidant	52:62	antioxidant	52:62	Retraction Note: Optimized extraction, composition, antioxidant and antimicrobial activities of exo and intracellular polysaccharides from submerged culture of Cordyceps cicadae.
30326910	0	13	theme	polysaccharides	118:132	arg1	activities					82:91	antimicrobial activities	68:91	antimicrobial activities	68:91	Retraction Note: Optimized extraction, composition, antioxidant and antimicrobial activities of exo and intracellular polysaccharides from submerged culture of Cordyceps cicadae.
30326910	0	13	theme	polysaccharides	118:132	arg1	composition					39:49	composition	39:49	composition	39:49	Retraction Note: Optimized extraction, composition, antioxidant and antimicrobial activities of exo and intracellular polysaccharides from submerged culture of Cordyceps cicadae.
30326910	0	13	theme	polysaccharides	118:132	arg1	extraction					27:36	extraction	27:36	extraction	27:36	Retraction Note: Optimized extraction, composition, antioxidant and antimicrobial activities of exo and intracellular polysaccharides from submerged culture of Cordyceps cicadae.
30326910	0	14	from	activities	82:91	arg1	culture					149:155	submerged culture	139:155	submerged culture of Cordyceps cicadae	139:176	Retraction Note: Optimized extraction, composition, antioxidant and antimicrobial activities of exo and intracellular polysaccharides from submerged culture of Cordyceps cicadae.
30326910	0	15	theme	submerged	139:147	arg1	culture					149:155	submerged culture	139:155	submerged culture of Cordyceps cicadae	139:176	Retraction Note: Optimized extraction, composition, antioxidant and antimicrobial activities of exo and intracellular polysaccharides from submerged culture of Cordyceps cicadae.
30326910	0	16	theme	Cordyceps	160:168	arg1	cicadae					170:176	Cordyceps cicadae	160:176	Cordyceps cicadae	160:176	Retraction Note: Optimized extraction, composition, antioxidant and antimicrobial activities of exo and intracellular polysaccharides from submerged culture of Cordyceps cicadae.
30326910	0	17	from	antioxidant	52:62	arg1	culture					149:155	submerged culture	139:155	submerged culture of Cordyceps cicadae	139:176	Retraction Note: Optimized extraction, composition, antioxidant and antimicrobial activities of exo and intracellular polysaccharides from submerged culture of Cordyceps cicadae.
30326910	2	18	theme	same	314:317	arg1	image					319:323	the same image	310:323	the same image representing different fungal mycelial growths	310:370	Both articles also appear to use the same image representing different fungal mycelial growths.
30326910	0	19	from	culture	149:155	arg1	antioxidant					52:62	antioxidant	52:62	antioxidant	52:62	Retraction Note: Optimized extraction, composition, antioxidant and antimicrobial activities of exo and intracellular polysaccharides from submerged culture of Cordyceps cicadae.
30326910	0	19	from	culture	149:155	arg1	activities					82:91	antimicrobial activities	68:91	antimicrobial activities	68:91	Retraction Note: Optimized extraction, composition, antioxidant and antimicrobial activities of exo and intracellular polysaccharides from submerged culture of Cordyceps cicadae.
30326910	0	19	from	culture	149:155	arg1	composition					39:49	composition	39:49	composition	39:49	Retraction Note: Optimized extraction, composition, antioxidant and antimicrobial activities of exo and intracellular polysaccharides from submerged culture of Cordyceps cicadae.
30326910	0	19	from	culture	149:155	arg1	polysaccharides					118:132	exo and intracellular polysaccharides	96:132	exo and intracellular polysaccharides from submerged culture of Cordyceps cicadae	96:176	Retraction Note: Optimized extraction, composition, antioxidant and antimicrobial activities of exo and intracellular polysaccharides from submerged culture of Cordyceps cicadae.
30326910	0	19	from	culture	149:155	arg1	extraction					27:36	extraction	27:36	extraction	27:36	Retraction Note: Optimized extraction, composition, antioxidant and antimicrobial activities of exo and intracellular polysaccharides from submerged culture of Cordyceps cicadae.
30326910	0	20	theme	cicadae	170:176	arg1	culture					149:155	submerged culture	139:155	submerged culture of Cordyceps cicadae	139:176	Retraction Note: Optimized extraction, composition, antioxidant and antimicrobial activities of exo and intracellular polysaccharides from submerged culture of Cordyceps cicadae.
30326910	0	21	dep	Note	11:14	arg1	antioxidant					52:62	antioxidant	52:62	antioxidant	52:62	Retraction Note: Optimized extraction, composition, antioxidant and antimicrobial activities of exo and intracellular polysaccharides from submerged culture of Cordyceps cicadae.
30326910	0	21	dep	Note	11:14	arg1	activities					82:91	antimicrobial activities	68:91	antimicrobial activities	68:91	Retraction Note: Optimized extraction, composition, antioxidant and antimicrobial activities of exo and intracellular polysaccharides from submerged culture of Cordyceps cicadae.
30326910	0	21	dep	Note	11:14	arg1	composition					39:49	composition	39:49	composition	39:49	Retraction Note: Optimized extraction, composition, antioxidant and antimicrobial activities of exo and intracellular polysaccharides from submerged culture of Cordyceps cicadae.
30326910	0	21	dep	Note	11:14	arg1	extraction					27:36	extraction	27:36	extraction	27:36	Retraction Note: Optimized extraction, composition, antioxidant and antimicrobial activities of exo and intracellular polysaccharides from submerged culture of Cordyceps cicadae.
30326910	2	22	theme	mycelial	355:362	arg1	growths					364:370	different fungal mycelial growths	338:370	different fungal mycelial growths	338:370	Both articles also appear to use the same image representing different fungal mycelial growths.
30326910	2	23	theme	fungal	348:353	arg1	growths					364:370	different fungal mycelial growths	338:370	different fungal mycelial growths	338:370	Both articles also appear to use the same image representing different fungal mycelial growths.
31614594	0	0	theme	RAW264.7	96:103	arg1	Cells					105:109	LPS-Stimulated RAW264.7 Cells	81:109	LPS-Stimulated RAW264.7 Cells	81:109	Anti-Inflammatory Effects of Lipids Extracted from Arctoscopus japonicus Eggs on LPS-Stimulated RAW264.7 Cells.
31614594	3	1	theme	A.	348:349	arg1	lipids					365:370	A. japonicus egg lipids	348:370	A. japonicus egg lipids	348:370	The results showed that A. japonicus egg lipids contained primarily polyunsaturated fatty acids (52.9% of the total fatty acid content; mostly eicosapentaenoic acid [EPA, 21.2 ± 0.5%] and docosahexaenoic acid [DHA, 25.9 ± 0.1%]), followed by monounsaturated fatty acids and saturated fatty acids (23.7% and 23.4%, respectively).
31614594	4	2	theme	IL-1β	808:812	arg1	macrophages					855:865	iNOS, COX-2, IL-1β, IL-6, and TNF-α LPS-stimulated RAW246.7 macrophages	795:865	iNOS, COX-2, IL-1β, IL-6, and TNF-α LPS-stimulated RAW246.7 macrophages	795:865	A. japonicus egg lipids significantly decreased nitric oxide (NO) production and suppressed the expression of immune-associated genes such as iNOS, COX-2, IL-1β, IL-6, and TNF-α LPS-stimulated RAW246.7 macrophages in dose-dependent manner.
31614594	6	3	theme	potential	1219:1227	arg1	biofunctions					1229:1240	potential biofunctions	1219:1240	potential biofunctions	1219:1240	These results indicate that the lipids extracted from A. japonicus eggs have potential biofunctions and might be useful for regulating inflammation in macrophages.
31614594	0	4	theme	LPS-Stimulated	81:94	arg1	Cells					105:109	LPS-Stimulated RAW264.7 Cells	81:109	LPS-Stimulated RAW264.7 Cells	81:109	Anti-Inflammatory Effects of Lipids Extracted from Arctoscopus japonicus Eggs on LPS-Stimulated RAW264.7 Cells.
31614594	5	5	theme	key	994:996	arg1	components					998:1007	key components	994:1007	key components of the NF-κB	994:1020	A. japonicus egg lipids also reduced the phosphorylation levels of NF-κB p-65, p38, ERK1/2, and JNK, key components of the NF-κB and MAPK pathways, suggesting that the lipid-induced anti-inflammatory activity is related to these signaling pathways.
31614594	6	6	theme	A.	1196:1197	arg1	eggs					1209:1212	A. japonicus eggs	1196:1212	A. japonicus eggs	1196:1212	These results indicate that the lipids extracted from A. japonicus eggs have potential biofunctions and might be useful for regulating inflammation in macrophages.
31614594	5	7	theme	signaling	1122:1130	arg1	pathways					1132:1139	these signaling pathways	1116:1139	these signaling pathways	1116:1139	A. japonicus egg lipids also reduced the phosphorylation levels of NF-κB p-65, p38, ERK1/2, and JNK, key components of the NF-κB and MAPK pathways, suggesting that the lipid-induced anti-inflammatory activity is related to these signaling pathways.
31614594	2	8	from	composition	209:219	arg1	macrophages					311:321	LPS-stimulated RAW246.7 macrophages	287:321	LPS-stimulated RAW246.7 macrophages	287:321	The present study investigated the fatty acid composition of A. japonicus egg lipids and their anti-inflammatory effects on LPS-stimulated RAW246.7 macrophages.
31614594	5	9	theme	phosphorylation	934:948	arg1	levels					950:955	the phosphorylation levels	930:955	the phosphorylation levels of NF-κB p-65, p38, ERK1/2, and JNK	930:991	A. japonicus egg lipids also reduced the phosphorylation levels of NF-κB p-65, p38, ERK1/2, and JNK, key components of the NF-κB and MAPK pathways, suggesting that the lipid-induced anti-inflammatory activity is related to these signaling pathways.
31614594	4	10	theme	IL-6	815:818	arg1	macrophages					855:865	iNOS, COX-2, IL-1β, IL-6, and TNF-α LPS-stimulated RAW246.7 macrophages	795:865	iNOS, COX-2, IL-1β, IL-6, and TNF-α LPS-stimulated RAW246.7 macrophages	795:865	A. japonicus egg lipids significantly decreased nitric oxide (NO) production and suppressed the expression of immune-associated genes such as iNOS, COX-2, IL-1β, IL-6, and TNF-α LPS-stimulated RAW246.7 macrophages in dose-dependent manner.
31614594	3	11	theme	25.9	539:542	arg1	±					544:544	±	544:544	±	544:544	The results showed that A. japonicus egg lipids contained primarily polyunsaturated fatty acids (52.9% of the total fatty acid content; mostly eicosapentaenoic acid [EPA, 21.2 ± 0.5%] and docosahexaenoic acid [DHA, 25.9 ± 0.1%]), followed by monounsaturated fatty acids and saturated fatty acids (23.7% and 23.4%, respectively).
31614594	4	12	dep	A.	653:654	arg1	japonicus					656:664	japonicus	656:664	japonicus	656:664	A. japonicus egg lipids significantly decreased nitric oxide (NO) production and suppressed the expression of immune-associated genes such as iNOS, COX-2, IL-1β, IL-6, and TNF-α LPS-stimulated RAW246.7 macrophages in dose-dependent manner.
31614594	3	13	theme	fatty	408:412	arg1	%					425:425	52.9%	421:425	52.9% of the total fatty acid content	421:457	The results showed that A. japonicus egg lipids contained primarily polyunsaturated fatty acids (52.9% of the total fatty acid content; mostly eicosapentaenoic acid [EPA, 21.2 ± 0.5%] and docosahexaenoic acid [DHA, 25.9 ± 0.1%]), followed by monounsaturated fatty acids and saturated fatty acids (23.7% and 23.4%, respectively).
31614594	3	13	theme	fatty	408:412	arg1	content					451:457	the total fatty acid content	430:457	the total fatty acid content	430:457	The results showed that A. japonicus egg lipids contained primarily polyunsaturated fatty acids (52.9% of the total fatty acid content; mostly eicosapentaenoic acid [EPA, 21.2 ± 0.5%] and docosahexaenoic acid [DHA, 25.9 ± 0.1%]), followed by monounsaturated fatty acids and saturated fatty acids (23.7% and 23.4%, respectively).
31614594	3	13	theme	fatty	408:412	arg1	[DHA					533:536	docosahexaenoic acid [DHA	512:536	docosahexaenoic acid [DHA	512:536	The results showed that A. japonicus egg lipids contained primarily polyunsaturated fatty acids (52.9% of the total fatty acid content; mostly eicosapentaenoic acid [EPA, 21.2 ± 0.5%] and docosahexaenoic acid [DHA, 25.9 ± 0.1%]), followed by monounsaturated fatty acids and saturated fatty acids (23.7% and 23.4%, respectively).
31614594	3	13	theme	fatty	408:412	arg1	%					505:505	21.2 ± 0.5%]	495:506	21.2 ± 0.5%]	495:506	The results showed that A. japonicus egg lipids contained primarily polyunsaturated fatty acids (52.9% of the total fatty acid content; mostly eicosapentaenoic acid [EPA, 21.2 ± 0.5%] and docosahexaenoic acid [DHA, 25.9 ± 0.1%]), followed by monounsaturated fatty acids and saturated fatty acids (23.7% and 23.4%, respectively).
31614594	3	13	theme	fatty	408:412	arg1	acids					414:418	polyunsaturated fatty acids	392:418	polyunsaturated fatty acids (52.9% of the total fatty acid content; mostly eicosapentaenoic acid [EPA, 21.2 ± 0.5%] and docosahexaenoic acid [DHA, 25.9 ± 0.1%])	392:551	The results showed that A. japonicus egg lipids contained primarily polyunsaturated fatty acids (52.9% of the total fatty acid content; mostly eicosapentaenoic acid [EPA, 21.2 ± 0.5%] and docosahexaenoic acid [DHA, 25.9 ± 0.1%]), followed by monounsaturated fatty acids and saturated fatty acids (23.7% and 23.4%, respectively).
31614594	3	13	theme	fatty	408:412	arg1	[EPA					489:492	mostly eicosapentaenoic acid [EPA	460:492	mostly eicosapentaenoic acid [EPA	460:492	The results showed that A. japonicus egg lipids contained primarily polyunsaturated fatty acids (52.9% of the total fatty acid content; mostly eicosapentaenoic acid [EPA, 21.2 ± 0.5%] and docosahexaenoic acid [DHA, 25.9 ± 0.1%]), followed by monounsaturated fatty acids and saturated fatty acids (23.7% and 23.4%, respectively).
31614594	2	14	theme	present	167:173	arg1	study					175:179	The present study	163:179	The present study	163:179	The present study investigated the fatty acid composition of A. japonicus egg lipids and their anti-inflammatory effects on LPS-stimulated RAW246.7 macrophages.
31614594	3	15	dep	%	425:425	arg1	%					549:549	25.9 ± 0.1%	539:549	25.9 ± 0.1%	539:549	The results showed that A. japonicus egg lipids contained primarily polyunsaturated fatty acids (52.9% of the total fatty acid content; mostly eicosapentaenoic acid [EPA, 21.2 ± 0.5%] and docosahexaenoic acid [DHA, 25.9 ± 0.1%]), followed by monounsaturated fatty acids and saturated fatty acids (23.7% and 23.4%, respectively).
31614594	3	16	theme	±	500:500	arg1	acids					414:418	polyunsaturated fatty acids	392:418	polyunsaturated fatty acids (52.9% of the total fatty acid content; mostly eicosapentaenoic acid [EPA, 21.2 ± 0.5%] and docosahexaenoic acid [DHA, 25.9 ± 0.1%])	392:551	The results showed that A. japonicus egg lipids contained primarily polyunsaturated fatty acids (52.9% of the total fatty acid content; mostly eicosapentaenoic acid [EPA, 21.2 ± 0.5%] and docosahexaenoic acid [DHA, 25.9 ± 0.1%]), followed by monounsaturated fatty acids and saturated fatty acids (23.7% and 23.4%, respectively).
31614594	3	16	theme	±	500:500	arg1	%					505:505	21.2 ± 0.5%]	495:506	21.2 ± 0.5%]	495:506	The results showed that A. japonicus egg lipids contained primarily polyunsaturated fatty acids (52.9% of the total fatty acid content; mostly eicosapentaenoic acid [EPA, 21.2 ± 0.5%] and docosahexaenoic acid [DHA, 25.9 ± 0.1%]), followed by monounsaturated fatty acids and saturated fatty acids (23.7% and 23.4%, respectively).
31614594	3	16	theme	±	500:500	arg1	content					451:457	the total fatty acid content	430:457	the total fatty acid content	430:457	The results showed that A. japonicus egg lipids contained primarily polyunsaturated fatty acids (52.9% of the total fatty acid content; mostly eicosapentaenoic acid [EPA, 21.2 ± 0.5%] and docosahexaenoic acid [DHA, 25.9 ± 0.1%]), followed by monounsaturated fatty acids and saturated fatty acids (23.7% and 23.4%, respectively).
31614594	6	17	from	inflammation	1277:1288	arg1	macrophages					1293:1303	macrophages	1293:1303	macrophages	1293:1303	These results indicate that the lipids extracted from A. japonicus eggs have potential biofunctions and might be useful for regulating inflammation in macrophages.
31614594	4	18	theme	iNOS	795:798	arg1	macrophages					855:865	iNOS, COX-2, IL-1β, IL-6, and TNF-α LPS-stimulated RAW246.7 macrophages	795:865	iNOS, COX-2, IL-1β, IL-6, and TNF-α LPS-stimulated RAW246.7 macrophages	795:865	A. japonicus egg lipids significantly decreased nitric oxide (NO) production and suppressed the expression of immune-associated genes such as iNOS, COX-2, IL-1β, IL-6, and TNF-α LPS-stimulated RAW246.7 macrophages in dose-dependent manner.
31614594	2	19	theme	RAW246.7	302:309	arg1	macrophages					311:321	LPS-stimulated RAW246.7 macrophages	287:321	LPS-stimulated RAW246.7 macrophages	287:321	The present study investigated the fatty acid composition of A. japonicus egg lipids and their anti-inflammatory effects on LPS-stimulated RAW246.7 macrophages.
31614594	5	20	theme	NF-κB	1016:1020	arg1	levels					950:955	the phosphorylation levels	930:955	the phosphorylation levels of NF-κB p-65, p38, ERK1/2, and JNK	930:991	A. japonicus egg lipids also reduced the phosphorylation levels of NF-κB p-65, p38, ERK1/2, and JNK, key components of the NF-κB and MAPK pathways, suggesting that the lipid-induced anti-inflammatory activity is related to these signaling pathways.
31614594	5	20	theme	NF-κB	1016:1020	arg1	pathways					1031:1038	MAPK pathways	1026:1038	MAPK pathways	1026:1038	A. japonicus egg lipids also reduced the phosphorylation levels of NF-κB p-65, p38, ERK1/2, and JNK, key components of the NF-κB and MAPK pathways, suggesting that the lipid-induced anti-inflammatory activity is related to these signaling pathways.
31614594	5	20	theme	NF-κB	1016:1020	arg1	components					998:1007	key components	994:1007	key components of the NF-κB	994:1020	A. japonicus egg lipids also reduced the phosphorylation levels of NF-κB p-65, p38, ERK1/2, and JNK, key components of the NF-κB and MAPK pathways, suggesting that the lipid-induced anti-inflammatory activity is related to these signaling pathways.
31614594	5	21	dep	A.	893:894	arg1	japonicus					896:904	japonicus	896:904	japonicus	896:904	A. japonicus egg lipids also reduced the phosphorylation levels of NF-κB p-65, p38, ERK1/2, and JNK, key components of the NF-κB and MAPK pathways, suggesting that the lipid-induced anti-inflammatory activity is related to these signaling pathways.
31614594	2	22	theme	LPS-stimulated	287:300	arg1	macrophages					311:321	LPS-stimulated RAW246.7 macrophages	287:321	LPS-stimulated RAW246.7 macrophages	287:321	The present study investigated the fatty acid composition of A. japonicus egg lipids and their anti-inflammatory effects on LPS-stimulated RAW246.7 macrophages.
31614594	4	23	theme	COX-2	801:805	arg1	macrophages					855:865	iNOS, COX-2, IL-1β, IL-6, and TNF-α LPS-stimulated RAW246.7 macrophages	795:865	iNOS, COX-2, IL-1β, IL-6, and TNF-α LPS-stimulated RAW246.7 macrophages	795:865	A. japonicus egg lipids significantly decreased nitric oxide (NO) production and suppressed the expression of immune-associated genes such as iNOS, COX-2, IL-1β, IL-6, and TNF-α LPS-stimulated RAW246.7 macrophages in dose-dependent manner.
31614594	5	24	theme	A.	893:894	arg1	lipids					910:915	A. japonicus egg lipids	893:915	A. japonicus egg lipids	893:915	A. japonicus egg lipids also reduced the phosphorylation levels of NF-κB p-65, p38, ERK1/2, and JNK, key components of the NF-κB and MAPK pathways, suggesting that the lipid-induced anti-inflammatory activity is related to these signaling pathways.
31614594	6	25	contain	have	1214:1217	arg1	useful					1255:1260	useful	1255:1260	useful	1255:1260	These results indicate that the lipids extracted from A. japonicus eggs have potential biofunctions and might be useful for regulating inflammation in macrophages.
31614594	6	25	contain	have	1214:1217	arg1	lipids					1174:1179	the lipids	1170:1179	the lipids extracted from A. japonicus eggs	1170:1212	These results indicate that the lipids extracted from A. japonicus eggs have potential biofunctions and might be useful for regulating inflammation in macrophages.
31614594	6	25	contain	have	1214:1217	arg2	biofunctions					1229:1240	potential biofunctions	1219:1240	potential biofunctions	1219:1240	These results indicate that the lipids extracted from A. japonicus eggs have potential biofunctions and might be useful for regulating inflammation in macrophages.
31614594	3	26	theme	acid	446:449	arg1	content					451:457	the total fatty acid content	430:457	the total fatty acid content	430:457	The results showed that A. japonicus egg lipids contained primarily polyunsaturated fatty acids (52.9% of the total fatty acid content; mostly eicosapentaenoic acid [EPA, 21.2 ± 0.5%] and docosahexaenoic acid [DHA, 25.9 ± 0.1%]), followed by monounsaturated fatty acids and saturated fatty acids (23.7% and 23.4%, respectively).
31614594	0	27	theme	Anti-Inflammatory	0:16	arg1	Effects					18:24	Anti-Inflammatory Effects	0:24	Anti-Inflammatory Effects of Lipids	0:34	Anti-Inflammatory Effects of Lipids Extracted from Arctoscopus japonicus Eggs on LPS-Stimulated RAW264.7 Cells.
31614594	4	28	theme	egg	666:668	arg1	lipids					670:675	A. japonicus egg lipids	653:675	A. japonicus egg lipids	653:675	A. japonicus egg lipids significantly decreased nitric oxide (NO) production and suppressed the expression of immune-associated genes such as iNOS, COX-2, IL-1β, IL-6, and TNF-α LPS-stimulated RAW246.7 macrophages in dose-dependent manner.
31614594	3	29	theme	content	451:457	arg1	%					425:425	52.9%	421:425	52.9% of the total fatty acid content	421:457	The results showed that A. japonicus egg lipids contained primarily polyunsaturated fatty acids (52.9% of the total fatty acid content; mostly eicosapentaenoic acid [EPA, 21.2 ± 0.5%] and docosahexaenoic acid [DHA, 25.9 ± 0.1%]), followed by monounsaturated fatty acids and saturated fatty acids (23.7% and 23.4%, respectively).
31614594	3	29	theme	content	451:457	arg1	content					451:457	the total fatty acid content	430:457	the total fatty acid content	430:457	The results showed that A. japonicus egg lipids contained primarily polyunsaturated fatty acids (52.9% of the total fatty acid content; mostly eicosapentaenoic acid [EPA, 21.2 ± 0.5%] and docosahexaenoic acid [DHA, 25.9 ± 0.1%]), followed by monounsaturated fatty acids and saturated fatty acids (23.7% and 23.4%, respectively).
31614594	3	29	theme	content	451:457	arg1	[DHA					533:536	docosahexaenoic acid [DHA	512:536	docosahexaenoic acid [DHA	512:536	The results showed that A. japonicus egg lipids contained primarily polyunsaturated fatty acids (52.9% of the total fatty acid content; mostly eicosapentaenoic acid [EPA, 21.2 ± 0.5%] and docosahexaenoic acid [DHA, 25.9 ± 0.1%]), followed by monounsaturated fatty acids and saturated fatty acids (23.7% and 23.4%, respectively).
31614594	3	29	theme	content	451:457	arg1	%					505:505	21.2 ± 0.5%]	495:506	21.2 ± 0.5%]	495:506	The results showed that A. japonicus egg lipids contained primarily polyunsaturated fatty acids (52.9% of the total fatty acid content; mostly eicosapentaenoic acid [EPA, 21.2 ± 0.5%] and docosahexaenoic acid [DHA, 25.9 ± 0.1%]), followed by monounsaturated fatty acids and saturated fatty acids (23.7% and 23.4%, respectively).
31614594	3	29	theme	content	451:457	arg1	acids					414:418	polyunsaturated fatty acids	392:418	polyunsaturated fatty acids (52.9% of the total fatty acid content; mostly eicosapentaenoic acid [EPA, 21.2 ± 0.5%] and docosahexaenoic acid [DHA, 25.9 ± 0.1%])	392:551	The results showed that A. japonicus egg lipids contained primarily polyunsaturated fatty acids (52.9% of the total fatty acid content; mostly eicosapentaenoic acid [EPA, 21.2 ± 0.5%] and docosahexaenoic acid [DHA, 25.9 ± 0.1%]), followed by monounsaturated fatty acids and saturated fatty acids (23.7% and 23.4%, respectively).
31614594	3	29	theme	content	451:457	arg1	[EPA					489:492	mostly eicosapentaenoic acid [EPA	460:492	mostly eicosapentaenoic acid [EPA	460:492	The results showed that A. japonicus egg lipids contained primarily polyunsaturated fatty acids (52.9% of the total fatty acid content; mostly eicosapentaenoic acid [EPA, 21.2 ± 0.5%] and docosahexaenoic acid [DHA, 25.9 ± 0.1%]), followed by monounsaturated fatty acids and saturated fatty acids (23.7% and 23.4%, respectively).
31614594	0	30	theme	Lipids	29:34	arg1	Effects					18:24	Anti-Inflammatory Effects	0:24	Anti-Inflammatory Effects of Lipids	0:34	Anti-Inflammatory Effects of Lipids Extracted from Arctoscopus japonicus Eggs on LPS-Stimulated RAW264.7 Cells.
31614594	3	31	theme	fatty	582:586	arg1	acids					588:592	monounsaturated fatty acids	566:592	monounsaturated fatty acids	566:592	The results showed that A. japonicus egg lipids contained primarily polyunsaturated fatty acids (52.9% of the total fatty acid content; mostly eicosapentaenoic acid [EPA, 21.2 ± 0.5%] and docosahexaenoic acid [DHA, 25.9 ± 0.1%]), followed by monounsaturated fatty acids and saturated fatty acids (23.7% and 23.4%, respectively).
31614594	3	32	dep	A.	348:349	arg1	japonicus					351:359	japonicus	351:359	japonicus	351:359	The results showed that A. japonicus egg lipids contained primarily polyunsaturated fatty acids (52.9% of the total fatty acid content; mostly eicosapentaenoic acid [EPA, 21.2 ± 0.5%] and docosahexaenoic acid [DHA, 25.9 ± 0.1%]), followed by monounsaturated fatty acids and saturated fatty acids (23.7% and 23.4%, respectively).
31614594	3	33	theme	0.1	546:548	arg1	±					544:544	±	544:544	±	544:544	The results showed that A. japonicus egg lipids contained primarily polyunsaturated fatty acids (52.9% of the total fatty acid content; mostly eicosapentaenoic acid [EPA, 21.2 ± 0.5%] and docosahexaenoic acid [DHA, 25.9 ± 0.1%]), followed by monounsaturated fatty acids and saturated fatty acids (23.7% and 23.4%, respectively).
31614594	5	34	theme	NF-κB	960:964	arg1	levels					950:955	the phosphorylation levels	930:955	the phosphorylation levels of NF-κB p-65, p38, ERK1/2, and JNK	930:991	A. japonicus egg lipids also reduced the phosphorylation levels of NF-κB p-65, p38, ERK1/2, and JNK, key components of the NF-κB and MAPK pathways, suggesting that the lipid-induced anti-inflammatory activity is related to these signaling pathways.
31614594	5	34	theme	NF-κB	960:964	arg1	pathways					1031:1038	MAPK pathways	1026:1038	MAPK pathways	1026:1038	A. japonicus egg lipids also reduced the phosphorylation levels of NF-κB p-65, p38, ERK1/2, and JNK, key components of the NF-κB and MAPK pathways, suggesting that the lipid-induced anti-inflammatory activity is related to these signaling pathways.
31614594	5	34	theme	NF-κB	960:964	arg1	components					998:1007	key components	994:1007	key components of the NF-κB	994:1020	A. japonicus egg lipids also reduced the phosphorylation levels of NF-κB p-65, p38, ERK1/2, and JNK, key components of the NF-κB and MAPK pathways, suggesting that the lipid-induced anti-inflammatory activity is related to these signaling pathways.
31614594	5	35	theme	egg	906:908	arg1	lipids					910:915	A. japonicus egg lipids	893:915	A. japonicus egg lipids	893:915	A. japonicus egg lipids also reduced the phosphorylation levels of NF-κB p-65, p38, ERK1/2, and JNK, key components of the NF-κB and MAPK pathways, suggesting that the lipid-induced anti-inflammatory activity is related to these signaling pathways.
31614594	4	36	theme	RAW246.7	846:853	arg1	macrophages					855:865	iNOS, COX-2, IL-1β, IL-6, and TNF-α LPS-stimulated RAW246.7 macrophages	795:865	iNOS, COX-2, IL-1β, IL-6, and TNF-α LPS-stimulated RAW246.7 macrophages	795:865	A. japonicus egg lipids significantly decreased nitric oxide (NO) production and suppressed the expression of immune-associated genes such as iNOS, COX-2, IL-1β, IL-6, and TNF-α LPS-stimulated RAW246.7 macrophages in dose-dependent manner.
31614594	2	37	theme	fatty	198:202	arg1	composition					209:219	the fatty acid composition	194:219	the fatty acid composition of A. japonicus egg lipids	194:246	The present study investigated the fatty acid composition of A. japonicus egg lipids and their anti-inflammatory effects on LPS-stimulated RAW246.7 macrophages.
31614594	4	38	theme	nitric	701:706	arg1	NO					715:716	NO	715:716	NO	715:716	A. japonicus egg lipids significantly decreased nitric oxide (NO) production and suppressed the expression of immune-associated genes such as iNOS, COX-2, IL-1β, IL-6, and TNF-α LPS-stimulated RAW246.7 macrophages in dose-dependent manner.
31614594	4	38	theme	nitric	701:706	arg1	oxide					708:712	nitric oxide	701:712	nitric oxide (NO) production	701:728	A. japonicus egg lipids significantly decreased nitric oxide (NO) production and suppressed the expression of immune-associated genes such as iNOS, COX-2, IL-1β, IL-6, and TNF-α LPS-stimulated RAW246.7 macrophages in dose-dependent manner.
31614594	3	39	dep	acids	588:592	arg1	%					625:625	23.7%	621:625	23.7%	621:625	The results showed that A. japonicus egg lipids contained primarily polyunsaturated fatty acids (52.9% of the total fatty acid content; mostly eicosapentaenoic acid [EPA, 21.2 ± 0.5%] and docosahexaenoic acid [DHA, 25.9 ± 0.1%]), followed by monounsaturated fatty acids and saturated fatty acids (23.7% and 23.4%, respectively).
31614594	3	39	dep	acids	588:592	arg1	%					635:635	23.4%	631:635	23.4%	631:635	The results showed that A. japonicus egg lipids contained primarily polyunsaturated fatty acids (52.9% of the total fatty acid content; mostly eicosapentaenoic acid [EPA, 21.2 ± 0.5%] and docosahexaenoic acid [DHA, 25.9 ± 0.1%]), followed by monounsaturated fatty acids and saturated fatty acids (23.7% and 23.4%, respectively).
31614594	3	40	theme	total	434:438	arg1	content					451:457	the total fatty acid content	430:457	the total fatty acid content	430:457	The results showed that A. japonicus egg lipids contained primarily polyunsaturated fatty acids (52.9% of the total fatty acid content; mostly eicosapentaenoic acid [EPA, 21.2 ± 0.5%] and docosahexaenoic acid [DHA, 25.9 ± 0.1%]), followed by monounsaturated fatty acids and saturated fatty acids (23.7% and 23.4%, respectively).
31614594	4	41	theme	dose-dependent	870:883	arg1	manner					885:890	dose-dependent manner	870:890	dose-dependent manner	870:890	A. japonicus egg lipids significantly decreased nitric oxide (NO) production and suppressed the expression of immune-associated genes such as iNOS, COX-2, IL-1β, IL-6, and TNF-α LPS-stimulated RAW246.7 macrophages in dose-dependent manner.
31614594	4	42	theme	immune-associated	763:779	arg1	macrophages					855:865	iNOS, COX-2, IL-1β, IL-6, and TNF-α LPS-stimulated RAW246.7 macrophages	795:865	iNOS, COX-2, IL-1β, IL-6, and TNF-α LPS-stimulated RAW246.7 macrophages	795:865	A. japonicus egg lipids significantly decreased nitric oxide (NO) production and suppressed the expression of immune-associated genes such as iNOS, COX-2, IL-1β, IL-6, and TNF-α LPS-stimulated RAW246.7 macrophages in dose-dependent manner.
31614594	4	42	theme	immune-associated	763:779	arg1	genes					781:785	immune-associated genes	763:785	immune-associated genes such as iNOS, COX-2, IL-1β, IL-6, and TNF-α LPS-stimulated RAW246.7 macrophages	763:865	A. japonicus egg lipids significantly decreased nitric oxide (NO) production and suppressed the expression of immune-associated genes such as iNOS, COX-2, IL-1β, IL-6, and TNF-α LPS-stimulated RAW246.7 macrophages in dose-dependent manner.
31614594	2	43	from	effects	276:282	arg1	macrophages					311:321	LPS-stimulated RAW246.7 macrophages	287:321	LPS-stimulated RAW246.7 macrophages	287:321	The present study investigated the fatty acid composition of A. japonicus egg lipids and their anti-inflammatory effects on LPS-stimulated RAW246.7 macrophages.
31614594	3	44	theme	eicosapentaenoic	467:482	arg1	acids					414:418	polyunsaturated fatty acids	392:418	polyunsaturated fatty acids (52.9% of the total fatty acid content; mostly eicosapentaenoic acid [EPA, 21.2 ± 0.5%] and docosahexaenoic acid [DHA, 25.9 ± 0.1%])	392:551	The results showed that A. japonicus egg lipids contained primarily polyunsaturated fatty acids (52.9% of the total fatty acid content; mostly eicosapentaenoic acid [EPA, 21.2 ± 0.5%] and docosahexaenoic acid [DHA, 25.9 ± 0.1%]), followed by monounsaturated fatty acids and saturated fatty acids (23.7% and 23.4%, respectively).
31614594	3	44	theme	eicosapentaenoic	467:482	arg1	[EPA					489:492	mostly eicosapentaenoic acid [EPA	460:492	mostly eicosapentaenoic acid [EPA	460:492	The results showed that A. japonicus egg lipids contained primarily polyunsaturated fatty acids (52.9% of the total fatty acid content; mostly eicosapentaenoic acid [EPA, 21.2 ± 0.5%] and docosahexaenoic acid [DHA, 25.9 ± 0.1%]), followed by monounsaturated fatty acids and saturated fatty acids (23.7% and 23.4%, respectively).
31614594	3	45	theme	fatty	440:444	arg1	acid					446:449	fatty acid	440:449	the total fatty acid content	430:457	The results showed that A. japonicus egg lipids contained primarily polyunsaturated fatty acids (52.9% of the total fatty acid content; mostly eicosapentaenoic acid [EPA, 21.2 ± 0.5%] and docosahexaenoic acid [DHA, 25.9 ± 0.1%]), followed by monounsaturated fatty acids and saturated fatty acids (23.7% and 23.4%, respectively).
31614594	3	46	theme	fatty	608:612	arg1	acids					614:618	saturated fatty acids	598:618	saturated fatty acids	598:618	The results showed that A. japonicus egg lipids contained primarily polyunsaturated fatty acids (52.9% of the total fatty acid content; mostly eicosapentaenoic acid [EPA, 21.2 ± 0.5%] and docosahexaenoic acid [DHA, 25.9 ± 0.1%]), followed by monounsaturated fatty acids and saturated fatty acids (23.7% and 23.4%, respectively).
31614594	2	47	theme	lipids	241:246	arg1	composition					209:219	the fatty acid composition	194:219	the fatty acid composition of A. japonicus egg lipids	194:246	The present study investigated the fatty acid composition of A. japonicus egg lipids and their anti-inflammatory effects on LPS-stimulated RAW246.7 macrophages.
31614594	2	47	theme	lipids	241:246	arg1	effects					276:282	their anti-inflammatory effects	252:282	their anti-inflammatory effects on LPS-stimulated RAW246.7 macrophages	252:321	The present study investigated the fatty acid composition of A. japonicus egg lipids and their anti-inflammatory effects on LPS-stimulated RAW246.7 macrophages.
31614594	3	48	theme	acid	484:487	arg1	acids					414:418	polyunsaturated fatty acids	392:418	polyunsaturated fatty acids (52.9% of the total fatty acid content; mostly eicosapentaenoic acid [EPA, 21.2 ± 0.5%] and docosahexaenoic acid [DHA, 25.9 ± 0.1%])	392:551	The results showed that A. japonicus egg lipids contained primarily polyunsaturated fatty acids (52.9% of the total fatty acid content; mostly eicosapentaenoic acid [EPA, 21.2 ± 0.5%] and docosahexaenoic acid [DHA, 25.9 ± 0.1%]), followed by monounsaturated fatty acids and saturated fatty acids (23.7% and 23.4%, respectively).
31614594	3	48	theme	acid	484:487	arg1	[EPA					489:492	mostly eicosapentaenoic acid [EPA	460:492	mostly eicosapentaenoic acid [EPA	460:492	The results showed that A. japonicus egg lipids contained primarily polyunsaturated fatty acids (52.9% of the total fatty acid content; mostly eicosapentaenoic acid [EPA, 21.2 ± 0.5%] and docosahexaenoic acid [DHA, 25.9 ± 0.1%]), followed by monounsaturated fatty acids and saturated fatty acids (23.7% and 23.4%, respectively).
31614594	0	49	theme	japonicus	63:71	arg1	Eggs					73:76	Arctoscopus japonicus Eggs	51:76	Arctoscopus japonicus Eggs on LPS-Stimulated RAW264.7 Cells	51:109	Anti-Inflammatory Effects of Lipids Extracted from Arctoscopus japonicus Eggs on LPS-Stimulated RAW264.7 Cells.
31614594	3	50	theme	polyunsaturated	392:406	arg1	%					425:425	52.9%	421:425	52.9% of the total fatty acid content	421:457	The results showed that A. japonicus egg lipids contained primarily polyunsaturated fatty acids (52.9% of the total fatty acid content; mostly eicosapentaenoic acid [EPA, 21.2 ± 0.5%] and docosahexaenoic acid [DHA, 25.9 ± 0.1%]), followed by monounsaturated fatty acids and saturated fatty acids (23.7% and 23.4%, respectively).
31614594	3	50	theme	polyunsaturated	392:406	arg1	content					451:457	the total fatty acid content	430:457	the total fatty acid content	430:457	The results showed that A. japonicus egg lipids contained primarily polyunsaturated fatty acids (52.9% of the total fatty acid content; mostly eicosapentaenoic acid [EPA, 21.2 ± 0.5%] and docosahexaenoic acid [DHA, 25.9 ± 0.1%]), followed by monounsaturated fatty acids and saturated fatty acids (23.7% and 23.4%, respectively).
31614594	3	50	theme	polyunsaturated	392:406	arg1	[DHA					533:536	docosahexaenoic acid [DHA	512:536	docosahexaenoic acid [DHA	512:536	The results showed that A. japonicus egg lipids contained primarily polyunsaturated fatty acids (52.9% of the total fatty acid content; mostly eicosapentaenoic acid [EPA, 21.2 ± 0.5%] and docosahexaenoic acid [DHA, 25.9 ± 0.1%]), followed by monounsaturated fatty acids and saturated fatty acids (23.7% and 23.4%, respectively).
31614594	3	50	theme	polyunsaturated	392:406	arg1	%					505:505	21.2 ± 0.5%]	495:506	21.2 ± 0.5%]	495:506	The results showed that A. japonicus egg lipids contained primarily polyunsaturated fatty acids (52.9% of the total fatty acid content; mostly eicosapentaenoic acid [EPA, 21.2 ± 0.5%] and docosahexaenoic acid [DHA, 25.9 ± 0.1%]), followed by monounsaturated fatty acids and saturated fatty acids (23.7% and 23.4%, respectively).
31614594	3	50	theme	polyunsaturated	392:406	arg1	acids					414:418	polyunsaturated fatty acids	392:418	polyunsaturated fatty acids (52.9% of the total fatty acid content; mostly eicosapentaenoic acid [EPA, 21.2 ± 0.5%] and docosahexaenoic acid [DHA, 25.9 ± 0.1%])	392:551	The results showed that A. japonicus egg lipids contained primarily polyunsaturated fatty acids (52.9% of the total fatty acid content; mostly eicosapentaenoic acid [EPA, 21.2 ± 0.5%] and docosahexaenoic acid [DHA, 25.9 ± 0.1%]), followed by monounsaturated fatty acids and saturated fatty acids (23.7% and 23.4%, respectively).
31614594	3	50	theme	polyunsaturated	392:406	arg1	[EPA					489:492	mostly eicosapentaenoic acid [EPA	460:492	mostly eicosapentaenoic acid [EPA	460:492	The results showed that A. japonicus egg lipids contained primarily polyunsaturated fatty acids (52.9% of the total fatty acid content; mostly eicosapentaenoic acid [EPA, 21.2 ± 0.5%] and docosahexaenoic acid [DHA, 25.9 ± 0.1%]), followed by monounsaturated fatty acids and saturated fatty acids (23.7% and 23.4%, respectively).
31614594	0	51	from	Eggs	73:76	arg1	Cells					105:109	LPS-Stimulated RAW264.7 Cells	81:109	LPS-Stimulated RAW264.7 Cells	81:109	Anti-Inflammatory Effects of Lipids Extracted from Arctoscopus japonicus Eggs on LPS-Stimulated RAW264.7 Cells.
31614594	2	52	theme	egg	237:239	arg1	lipids					241:246	A. japonicus egg lipids	224:246	A. japonicus egg lipids	224:246	The present study investigated the fatty acid composition of A. japonicus egg lipids and their anti-inflammatory effects on LPS-stimulated RAW246.7 macrophages.
31614594	3	53	contain	contained	372:380	arg2	%					505:505	21.2 ± 0.5%]	495:506	21.2 ± 0.5%]	495:506	The results showed that A. japonicus egg lipids contained primarily polyunsaturated fatty acids (52.9% of the total fatty acid content; mostly eicosapentaenoic acid [EPA, 21.2 ± 0.5%] and docosahexaenoic acid [DHA, 25.9 ± 0.1%]), followed by monounsaturated fatty acids and saturated fatty acids (23.7% and 23.4%, respectively).
31614594	3	53	contain	contained	372:380	arg2	[DHA					533:536	docosahexaenoic acid [DHA	512:536	docosahexaenoic acid [DHA	512:536	The results showed that A. japonicus egg lipids contained primarily polyunsaturated fatty acids (52.9% of the total fatty acid content; mostly eicosapentaenoic acid [EPA, 21.2 ± 0.5%] and docosahexaenoic acid [DHA, 25.9 ± 0.1%]), followed by monounsaturated fatty acids and saturated fatty acids (23.7% and 23.4%, respectively).
31614594	3	53	contain	contained	372:380	arg2	[EPA					489:492	mostly eicosapentaenoic acid [EPA	460:492	mostly eicosapentaenoic acid [EPA	460:492	The results showed that A. japonicus egg lipids contained primarily polyunsaturated fatty acids (52.9% of the total fatty acid content; mostly eicosapentaenoic acid [EPA, 21.2 ± 0.5%] and docosahexaenoic acid [DHA, 25.9 ± 0.1%]), followed by monounsaturated fatty acids and saturated fatty acids (23.7% and 23.4%, respectively).
31614594	3	53	contain	contained	372:380	arg2	acids					414:418	polyunsaturated fatty acids	392:418	polyunsaturated fatty acids (52.9% of the total fatty acid content; mostly eicosapentaenoic acid [EPA, 21.2 ± 0.5%] and docosahexaenoic acid [DHA, 25.9 ± 0.1%])	392:551	The results showed that A. japonicus egg lipids contained primarily polyunsaturated fatty acids (52.9% of the total fatty acid content; mostly eicosapentaenoic acid [EPA, 21.2 ± 0.5%] and docosahexaenoic acid [DHA, 25.9 ± 0.1%]), followed by monounsaturated fatty acids and saturated fatty acids (23.7% and 23.4%, respectively).
31614594	3	53	contain	contained	372:380	arg2	%					425:425	52.9%	421:425	52.9% of the total fatty acid content	421:457	The results showed that A. japonicus egg lipids contained primarily polyunsaturated fatty acids (52.9% of the total fatty acid content; mostly eicosapentaenoic acid [EPA, 21.2 ± 0.5%] and docosahexaenoic acid [DHA, 25.9 ± 0.1%]), followed by monounsaturated fatty acids and saturated fatty acids (23.7% and 23.4%, respectively).
31614594	3	53	contain	contained	372:380	arg2	content					451:457	the total fatty acid content	430:457	the total fatty acid content	430:457	The results showed that A. japonicus egg lipids contained primarily polyunsaturated fatty acids (52.9% of the total fatty acid content; mostly eicosapentaenoic acid [EPA, 21.2 ± 0.5%] and docosahexaenoic acid [DHA, 25.9 ± 0.1%]), followed by monounsaturated fatty acids and saturated fatty acids (23.7% and 23.4%, respectively).
31614594	3	53	contain	contained	372:380	arg1	lipids					365:370	A. japonicus egg lipids	348:370	A. japonicus egg lipids	348:370	The results showed that A. japonicus egg lipids contained primarily polyunsaturated fatty acids (52.9% of the total fatty acid content; mostly eicosapentaenoic acid [EPA, 21.2 ± 0.5%] and docosahexaenoic acid [DHA, 25.9 ± 0.1%]), followed by monounsaturated fatty acids and saturated fatty acids (23.7% and 23.4%, respectively).
31614594	0	54	theme	Arctoscopus	51:61	arg1	Eggs					73:76	Arctoscopus japonicus Eggs	51:76	Arctoscopus japonicus Eggs on LPS-Stimulated RAW264.7 Cells	51:109	Anti-Inflammatory Effects of Lipids Extracted from Arctoscopus japonicus Eggs on LPS-Stimulated RAW264.7 Cells.
31614594	5	55	theme	lipid-induced	1061:1073	arg1	related					1105:1111	related	1105:1111	related	1105:1111	A. japonicus egg lipids also reduced the phosphorylation levels of NF-κB p-65, p38, ERK1/2, and JNK, key components of the NF-κB and MAPK pathways, suggesting that the lipid-induced anti-inflammatory activity is related to these signaling pathways.
31614594	5	55	theme	lipid-induced	1061:1073	arg1	activity					1093:1100	the lipid-induced anti-inflammatory activity	1057:1100	the lipid-induced anti-inflammatory activity	1057:1100	A. japonicus egg lipids also reduced the phosphorylation levels of NF-κB p-65, p38, ERK1/2, and JNK, key components of the NF-κB and MAPK pathways, suggesting that the lipid-induced anti-inflammatory activity is related to these signaling pathways.
31614594	2	56	theme	A.	224:225	arg1	lipids					241:246	A. japonicus egg lipids	224:246	A. japonicus egg lipids	224:246	The present study investigated the fatty acid composition of A. japonicus egg lipids and their anti-inflammatory effects on LPS-stimulated RAW246.7 macrophages.
31614594	4	57	theme	genes	781:785	arg1	expression					749:758	the expression	745:758	the expression of immune-associated genes such as iNOS, COX-2, IL-1β, IL-6, and TNF-α LPS-stimulated RAW246.7 macrophages in dose-dependent manner	745:890	A. japonicus egg lipids significantly decreased nitric oxide (NO) production and suppressed the expression of immune-associated genes such as iNOS, COX-2, IL-1β, IL-6, and TNF-α LPS-stimulated RAW246.7 macrophages in dose-dependent manner.
31614594	4	58	theme	A.	653:654	arg1	lipids					670:675	A. japonicus egg lipids	653:675	A. japonicus egg lipids	653:675	A. japonicus egg lipids significantly decreased nitric oxide (NO) production and suppressed the expression of immune-associated genes such as iNOS, COX-2, IL-1β, IL-6, and TNF-α LPS-stimulated RAW246.7 macrophages in dose-dependent manner.
31614594	5	59	theme	anti-inflammatory	1075:1091	arg1	related					1105:1111	related	1105:1111	related	1105:1111	A. japonicus egg lipids also reduced the phosphorylation levels of NF-κB p-65, p38, ERK1/2, and JNK, key components of the NF-κB and MAPK pathways, suggesting that the lipid-induced anti-inflammatory activity is related to these signaling pathways.
31614594	5	59	theme	anti-inflammatory	1075:1091	arg1	activity					1093:1100	the lipid-induced anti-inflammatory activity	1057:1100	the lipid-induced anti-inflammatory activity	1057:1100	A. japonicus egg lipids also reduced the phosphorylation levels of NF-κB p-65, p38, ERK1/2, and JNK, key components of the NF-κB and MAPK pathways, suggesting that the lipid-induced anti-inflammatory activity is related to these signaling pathways.
31614594	4	60	theme	LPS-stimulated	831:844	arg1	macrophages					855:865	iNOS, COX-2, IL-1β, IL-6, and TNF-α LPS-stimulated RAW246.7 macrophages	795:865	iNOS, COX-2, IL-1β, IL-6, and TNF-α LPS-stimulated RAW246.7 macrophages	795:865	A. japonicus egg lipids significantly decreased nitric oxide (NO) production and suppressed the expression of immune-associated genes such as iNOS, COX-2, IL-1β, IL-6, and TNF-α LPS-stimulated RAW246.7 macrophages in dose-dependent manner.
31614594	3	61	theme	monounsaturated	566:580	arg1	acids					588:592	monounsaturated fatty acids	566:592	monounsaturated fatty acids	566:592	The results showed that A. japonicus egg lipids contained primarily polyunsaturated fatty acids (52.9% of the total fatty acid content; mostly eicosapentaenoic acid [EPA, 21.2 ± 0.5%] and docosahexaenoic acid [DHA, 25.9 ± 0.1%]), followed by monounsaturated fatty acids and saturated fatty acids (23.7% and 23.4%, respectively).
31614594	1	62	theme	cold-water	139:148	arg1	fish					157:160	a cold-water marine fish	137:160	a cold-water marine fish	137:160	Arctoscopus japonicus is a cold-water marine fish.
31614594	1	62	theme	cold-water	139:148	arg1	japonicus					124:132	Arctoscopus japonicus	112:132	Arctoscopus japonicus	112:132	Arctoscopus japonicus is a cold-water marine fish.
31614594	4	63	theme	oxide	708:712	arg1	production					719:728	nitric oxide (NO) production	701:728	nitric oxide (NO) production	701:728	A. japonicus egg lipids significantly decreased nitric oxide (NO) production and suppressed the expression of immune-associated genes such as iNOS, COX-2, IL-1β, IL-6, and TNF-α LPS-stimulated RAW246.7 macrophages in dose-dependent manner.
31614594	4	64	theme	TNF-α	825:829	arg1	macrophages					855:865	iNOS, COX-2, IL-1β, IL-6, and TNF-α LPS-stimulated RAW246.7 macrophages	795:865	iNOS, COX-2, IL-1β, IL-6, and TNF-α LPS-stimulated RAW246.7 macrophages	795:865	A. japonicus egg lipids significantly decreased nitric oxide (NO) production and suppressed the expression of immune-associated genes such as iNOS, COX-2, IL-1β, IL-6, and TNF-α LPS-stimulated RAW246.7 macrophages in dose-dependent manner.
31614594	3	65	theme	saturated	598:606	arg1	acids					614:618	saturated fatty acids	598:618	saturated fatty acids	598:618	The results showed that A. japonicus egg lipids contained primarily polyunsaturated fatty acids (52.9% of the total fatty acid content; mostly eicosapentaenoic acid [EPA, 21.2 ± 0.5%] and docosahexaenoic acid [DHA, 25.9 ± 0.1%]), followed by monounsaturated fatty acids and saturated fatty acids (23.7% and 23.4%, respectively).
31614594	4	66	from	expression	749:758	arg1	manner					885:890	dose-dependent manner	870:890	dose-dependent manner	870:890	A. japonicus egg lipids significantly decreased nitric oxide (NO) production and suppressed the expression of immune-associated genes such as iNOS, COX-2, IL-1β, IL-6, and TNF-α LPS-stimulated RAW246.7 macrophages in dose-dependent manner.
31614594	1	67	theme	marine	150:155	arg1	fish					157:160	a cold-water marine fish	137:160	a cold-water marine fish	137:160	Arctoscopus japonicus is a cold-water marine fish.
31614594	1	67	theme	marine	150:155	arg1	japonicus					124:132	Arctoscopus japonicus	112:132	Arctoscopus japonicus	112:132	Arctoscopus japonicus is a cold-water marine fish.
31614594	3	68	theme	egg	361:363	arg1	lipids					365:370	A. japonicus egg lipids	348:370	A. japonicus egg lipids	348:370	The results showed that A. japonicus egg lipids contained primarily polyunsaturated fatty acids (52.9% of the total fatty acid content; mostly eicosapentaenoic acid [EPA, 21.2 ± 0.5%] and docosahexaenoic acid [DHA, 25.9 ± 0.1%]), followed by monounsaturated fatty acids and saturated fatty acids (23.7% and 23.4%, respectively).
31614594	2	69	theme	acid	204:207	arg1	composition					209:219	the fatty acid composition	194:219	the fatty acid composition of A. japonicus egg lipids	194:246	The present study investigated the fatty acid composition of A. japonicus egg lipids and their anti-inflammatory effects on LPS-stimulated RAW246.7 macrophages.
31614594	2	70	theme	anti-inflammatory	258:274	arg1	effects					276:282	their anti-inflammatory effects	252:282	their anti-inflammatory effects on LPS-stimulated RAW246.7 macrophages	252:321	The present study investigated the fatty acid composition of A. japonicus egg lipids and their anti-inflammatory effects on LPS-stimulated RAW246.7 macrophages.
31614594	3	71	theme	docosahexaenoic	512:526	arg1	acid					528:531	docosahexaenoic acid	512:531	docosahexaenoic acid [DHA	512:536	The results showed that A. japonicus egg lipids contained primarily polyunsaturated fatty acids (52.9% of the total fatty acid content; mostly eicosapentaenoic acid [EPA, 21.2 ± 0.5%] and docosahexaenoic acid [DHA, 25.9 ± 0.1%]), followed by monounsaturated fatty acids and saturated fatty acids (23.7% and 23.4%, respectively).
31614594	2	72	dep	A.	224:225	arg1	japonicus					227:235	japonicus	227:235	japonicus	227:235	The present study investigated the fatty acid composition of A. japonicus egg lipids and their anti-inflammatory effects on LPS-stimulated RAW246.7 macrophages.
31614594	5	73	theme	MAPK	1026:1029	arg1	pathways					1031:1038	MAPK pathways	1026:1038	MAPK pathways	1026:1038	A. japonicus egg lipids also reduced the phosphorylation levels of NF-κB p-65, p38, ERK1/2, and JNK, key components of the NF-κB and MAPK pathways, suggesting that the lipid-induced anti-inflammatory activity is related to these signaling pathways.
31614594	3	74	theme	acid	528:531	arg1	[DHA					533:536	docosahexaenoic acid [DHA	512:536	docosahexaenoic acid [DHA	512:536	The results showed that A. japonicus egg lipids contained primarily polyunsaturated fatty acids (52.9% of the total fatty acid content; mostly eicosapentaenoic acid [EPA, 21.2 ± 0.5%] and docosahexaenoic acid [DHA, 25.9 ± 0.1%]), followed by monounsaturated fatty acids and saturated fatty acids (23.7% and 23.4%, respectively).
31614594	3	74	theme	acid	528:531	arg1	acids					414:418	polyunsaturated fatty acids	392:418	polyunsaturated fatty acids (52.9% of the total fatty acid content; mostly eicosapentaenoic acid [EPA, 21.2 ± 0.5%] and docosahexaenoic acid [DHA, 25.9 ± 0.1%])	392:551	The results showed that A. japonicus egg lipids contained primarily polyunsaturated fatty acids (52.9% of the total fatty acid content; mostly eicosapentaenoic acid [EPA, 21.2 ± 0.5%] and docosahexaenoic acid [DHA, 25.9 ± 0.1%]), followed by monounsaturated fatty acids and saturated fatty acids (23.7% and 23.4%, respectively).
31614594	6	75	theme	japonicus	1199:1207	arg1	eggs					1209:1212	A. japonicus eggs	1196:1212	A. japonicus eggs	1196:1212	These results indicate that the lipids extracted from A. japonicus eggs have potential biofunctions and might be useful for regulating inflammation in macrophages.
31240650	9	0	theme	characteristics	1143:1157	arg1	knowledge					1116:1124	the knowledge	1112:1124	the knowledge of nitrification characteristics, auto-aggregation performance, and EPS compositions of TN-10 during NaCl shock	1112:1236	These results provided further insights into the knowledge of nitrification characteristics, auto-aggregation performance, and EPS compositions of TN-10 during NaCl shock.
31240650	7	1	theme	main	878:881	arg1	polysaccharides					853:867	polysaccharides	853:867	polysaccharides	853:867	Furthermore, proteins and polysaccharides were the main components of EPS, and the contents were greatly influenced by salinity.
31240650	7	1	theme	main	878:881	arg1	proteins					840:847	proteins	840:847	proteins	840:847	Furthermore, proteins and polysaccharides were the main components of EPS, and the contents were greatly influenced by salinity.
31240650	7	1	theme	main	878:881	arg1	components					883:892	the main components	874:892	the main components of EPS	874:899	Furthermore, proteins and polysaccharides were the main components of EPS, and the contents were greatly influenced by salinity.
31240650	2	2	theme	Klebsiella	122:131	arg1	sp					133:134	Klebsiella sp	122:134	Klebsiella sp.	122:135	Klebsiella sp.
31240650	3	3	theme	NaCl	245:248	arg1	contents					250:257	NaCl contents	245:257	NaCl contents ranging from 0 to 35 g/L	245:282	TN-10, isolated from tannery wastewater, exhibited excellent nitrification ability cultivated in media with NaCl contents ranging from 0 to 35 g/L.
31240650	6	4	theme	NaCl	802:805	arg1	shock					807:811	NaCl shock	802:811	NaCl shock	802:811	Extinction-emission matrix (EEM) and Fourier-transform infrared (FT-IR) spectrum analyses showed that the compositions of extracellular polymeric substances (EPS) by TN-10 after NaCl shock were similar.
31240650	0	5	theme	sp	111:112	arg1	characteristic					82:95	extracellular polymeric substance characteristic	48:95	extracellular polymeric substance characteristic of Klebsiella sp	48:112	Effect of NaCl on nitrification performance and extracellular polymeric substance characteristic of Klebsiella sp.
31240650	0	5	theme	sp	111:112	arg1	performance					32:42	nitrification performance	18:42	nitrification performance	18:42	Effect of NaCl on nitrification performance and extracellular polymeric substance characteristic of Klebsiella sp.
31240650	0	6	from	Effect	0:5	arg1	characteristic					82:95	extracellular polymeric substance characteristic	48:95	extracellular polymeric substance characteristic of Klebsiella sp	48:112	Effect of NaCl on nitrification performance and extracellular polymeric substance characteristic of Klebsiella sp.
31240650	0	6	from	Effect	0:5	arg1	performance					32:42	nitrification performance	18:42	nitrification performance	18:42	Effect of NaCl on nitrification performance and extracellular polymeric substance characteristic of Klebsiella sp.
31240650	8	7	theme	circular	962:969	arg1	CD					982:983	CD	982:983	CD	982:983	Then, circular dichroism (CD) spectrum was used to calculate the contents of each protein secondary structure.
31240650	8	7	theme	circular	962:969	arg1	dichroism					971:979	circular dichroism	962:979	circular dichroism (CD) spectrum	962:993	Then, circular dichroism (CD) spectrum was used to calculate the contents of each protein secondary structure.
31240650	0	8	theme	Klebsiella	100:109	arg1	sp					111:112	Klebsiella sp	100:112	Klebsiella sp	100:112	Effect of NaCl on nitrification performance and extracellular polymeric substance characteristic of Klebsiella sp.
31240650	4	9	theme	stress	459:464	arg1	conditions					466:475	salt stress conditions	454:475	salt stress conditions	454:475	Analysis of antioxidative enzyme activities of TN-10 stressed by NaCl showed that the stain produced more superoxide dismutase (SOD) and catalase (CAT) to fight against salt stress conditions.
31240650	6	10	theme	extracellular	746:758	arg1	EPS					782:784	EPS	782:784	EPS	782:784	Extinction-emission matrix (EEM) and Fourier-transform infrared (FT-IR) spectrum analyses showed that the compositions of extracellular polymeric substances (EPS) by TN-10 after NaCl shock were similar.
31240650	6	10	theme	extracellular	746:758	arg1	substances					770:779	extracellular polymeric substances	746:779	extracellular polymeric substances (EPS)	746:785	Extinction-emission matrix (EEM) and Fourier-transform infrared (FT-IR) spectrum analyses showed that the compositions of extracellular polymeric substances (EPS) by TN-10 after NaCl shock were similar.
31240650	3	11	theme	tannery	158:164	arg1	wastewater					166:175	tannery wastewater	158:175	tannery wastewater	158:175	TN-10, isolated from tannery wastewater, exhibited excellent nitrification ability cultivated in media with NaCl contents ranging from 0 to 35 g/L.
31240650	4	12	theme	antioxidative	297:309	arg1	activities					318:327	antioxidative enzyme activities	297:327	antioxidative enzyme activities of TN-10 stressed by NaCl	297:353	Analysis of antioxidative enzyme activities of TN-10 stressed by NaCl showed that the stain produced more superoxide dismutase (SOD) and catalase (CAT) to fight against salt stress conditions.
31240650	5	13	theme	auto-aggregation	492:507	arg1	index					509:513	the auto-aggregation index	488:513	the auto-aggregation index of TN-10	488:522	Moreover, the auto-aggregation index of TN-10 decreased with the increment of NaCl contents, which is consistent with results of zeta potentials.
31240650	8	14	theme	dichroism	971:979	arg1	spectrum					986:993	circular dichroism (CD) spectrum	962:993	circular dichroism (CD) spectrum	962:993	Then, circular dichroism (CD) spectrum was used to calculate the contents of each protein secondary structure.
31240650	4	15	theme	salt	454:457	arg1	conditions					466:475	salt stress conditions	454:475	salt stress conditions	454:475	Analysis of antioxidative enzyme activities of TN-10 stressed by NaCl showed that the stain produced more superoxide dismutase (SOD) and catalase (CAT) to fight against salt stress conditions.
31240650	5	16	theme	zeta	607:610	arg1	potentials					612:621	zeta potentials	607:621	zeta potentials	607:621	Moreover, the auto-aggregation index of TN-10 decreased with the increment of NaCl contents, which is consistent with results of zeta potentials.
31240650	5	17	theme	NaCl	556:559	arg1	contents					561:568	NaCl contents	556:568	NaCl contents	556:568	Moreover, the auto-aggregation index of TN-10 decreased with the increment of NaCl contents, which is consistent with results of zeta potentials.
31240650	4	18	theme	activities	318:327	arg1	Analysis					285:292	Analysis	285:292	Analysis of antioxidative enzyme activities of TN-10 stressed by NaCl	285:353	Analysis of antioxidative enzyme activities of TN-10 stressed by NaCl showed that the stain produced more superoxide dismutase (SOD) and catalase (CAT) to fight against salt stress conditions.
31240650	5	19	theme	potentials	612:621	arg1	results					596:602	results	596:602	results of zeta potentials	596:621	Moreover, the auto-aggregation index of TN-10 decreased with the increment of NaCl contents, which is consistent with results of zeta potentials.
31240650	5	20	theme	contents	561:568	arg1	consistent					580:589	consistent	580:589	consistent	580:589	Moreover, the auto-aggregation index of TN-10 decreased with the increment of NaCl contents, which is consistent with results of zeta potentials.
31240650	5	20	theme	contents	561:568	arg1	increment					543:551	the increment	539:551	the increment	539:551	Moreover, the auto-aggregation index of TN-10 decreased with the increment of NaCl contents, which is consistent with results of zeta potentials.
31240650	4	21	theme	enzyme	311:316	arg1	activities					318:327	antioxidative enzyme activities	297:327	antioxidative enzyme activities of TN-10 stressed by NaCl	297:353	Analysis of antioxidative enzyme activities of TN-10 stressed by NaCl showed that the stain produced more superoxide dismutase (SOD) and catalase (CAT) to fight against salt stress conditions.
31240650	3	22	theme	excellent	188:196	arg1	ability					212:218	excellent nitrification ability	188:218	excellent nitrification ability cultivated in media with NaCl contents ranging from 0 to 35 g/L	188:282	TN-10, isolated from tannery wastewater, exhibited excellent nitrification ability cultivated in media with NaCl contents ranging from 0 to 35 g/L.
31240650	6	23	theme	infrared	679:686	arg1	analyses					705:712	Extinction-emission matrix (EEM) and Fourier-transform infrared (FT-IR) spectrum analyses	624:712	analyses	705:712	Extinction-emission matrix (EEM) and Fourier-transform infrared (FT-IR) spectrum analyses showed that the compositions of extracellular polymeric substances (EPS) by TN-10 after NaCl shock were similar.
31240650	4	24	theme	superoxide	391:400	arg1	SOD					413:415	SOD	413:415	SOD	413:415	Analysis of antioxidative enzyme activities of TN-10 stressed by NaCl showed that the stain produced more superoxide dismutase (SOD) and catalase (CAT) to fight against salt stress conditions.
31240650	4	24	theme	superoxide	391:400	arg1	dismutase					402:410	more superoxide dismutase	386:410	more superoxide dismutase (SOD)	386:416	Analysis of antioxidative enzyme activities of TN-10 stressed by NaCl showed that the stain produced more superoxide dismutase (SOD) and catalase (CAT) to fight against salt stress conditions.
31240650	8	25	theme	protein	1038:1044	arg1	structure					1056:1064	each protein secondary structure	1033:1064	each protein secondary structure	1033:1064	Then, circular dichroism (CD) spectrum was used to calculate the contents of each protein secondary structure.
31240650	4	26	theme	more	386:389	arg1	SOD					413:415	SOD	413:415	SOD	413:415	Analysis of antioxidative enzyme activities of TN-10 stressed by NaCl showed that the stain produced more superoxide dismutase (SOD) and catalase (CAT) to fight against salt stress conditions.
31240650	4	26	theme	more	386:389	arg1	dismutase					402:410	more superoxide dismutase	386:410	more superoxide dismutase (SOD)	386:416	Analysis of antioxidative enzyme activities of TN-10 stressed by NaCl showed that the stain produced more superoxide dismutase (SOD) and catalase (CAT) to fight against salt stress conditions.
31240650	6	27	theme	Fourier-transform	661:677	arg1	analyses					705:712	Extinction-emission matrix (EEM) and Fourier-transform infrared (FT-IR) spectrum analyses	624:712	analyses	705:712	Extinction-emission matrix (EEM) and Fourier-transform infrared (FT-IR) spectrum analyses showed that the compositions of extracellular polymeric substances (EPS) by TN-10 after NaCl shock were similar.
31240650	0	28	theme	NaCl	10:13	arg1	Effect					0:5	Effect	0:5	Effect of NaCl on nitrification performance and extracellular polymeric substance characteristic of Klebsiella sp.	0:113	Effect of NaCl on nitrification performance and extracellular polymeric substance characteristic of Klebsiella sp.
31240650	9	29	theme	TN-10	1214:1218	arg1	performance					1177:1187	auto-aggregation performance	1160:1187	auto-aggregation performance	1160:1187	These results provided further insights into the knowledge of nitrification characteristics, auto-aggregation performance, and EPS compositions of TN-10 during NaCl shock.
31240650	9	29	theme	TN-10	1214:1218	arg1	compositions					1198:1209	EPS compositions	1194:1209	EPS compositions	1194:1209	These results provided further insights into the knowledge of nitrification characteristics, auto-aggregation performance, and EPS compositions of TN-10 during NaCl shock.
31240650	9	29	theme	TN-10	1214:1218	arg1	characteristics					1143:1157	nitrification characteristics	1129:1157	nitrification characteristics	1129:1157	These results provided further insights into the knowledge of nitrification characteristics, auto-aggregation performance, and EPS compositions of TN-10 during NaCl shock.
31240650	8	30	theme	structure	1056:1064	arg1	contents					1021:1028	the contents	1017:1028	the contents of each protein secondary structure	1017:1064	Then, circular dichroism (CD) spectrum was used to calculate the contents of each protein secondary structure.
31240650	0	31	theme	nitrification	18:30	arg1	performance					32:42	nitrification performance	18:42	nitrification performance	18:42	Effect of NaCl on nitrification performance and extracellular polymeric substance characteristic of Klebsiella sp.
31240650	8	32	theme	secondary	1046:1054	arg1	structure					1056:1064	each protein secondary structure	1033:1064	each protein secondary structure	1033:1064	Then, circular dichroism (CD) spectrum was used to calculate the contents of each protein secondary structure.
31240650	9	33	theme	NaCl	1227:1230	arg1	shock					1232:1236	NaCl shock	1227:1236	NaCl shock	1227:1236	These results provided further insights into the knowledge of nitrification characteristics, auto-aggregation performance, and EPS compositions of TN-10 during NaCl shock.
31240650	6	34	theme	polymeric	760:768	arg1	EPS					782:784	EPS	782:784	EPS	782:784	Extinction-emission matrix (EEM) and Fourier-transform infrared (FT-IR) spectrum analyses showed that the compositions of extracellular polymeric substances (EPS) by TN-10 after NaCl shock were similar.
31240650	6	34	theme	polymeric	760:768	arg1	substances					770:779	extracellular polymeric substances	746:779	extracellular polymeric substances (EPS)	746:785	Extinction-emission matrix (EEM) and Fourier-transform infrared (FT-IR) spectrum analyses showed that the compositions of extracellular polymeric substances (EPS) by TN-10 after NaCl shock were similar.
31240650	5	35	with	consistent	580:589	arg1	results					596:602	results	596:602	results of zeta potentials	596:621	Moreover, the auto-aggregation index of TN-10 decreased with the increment of NaCl contents, which is consistent with results of zeta potentials.
31240650	0	36	theme	extracellular	48:60	arg1	characteristic					82:95	extracellular polymeric substance characteristic	48:95	extracellular polymeric substance characteristic of Klebsiella sp	48:112	Effect of NaCl on nitrification performance and extracellular polymeric substance characteristic of Klebsiella sp.
31240650	9	37	theme	performance	1177:1187	arg1	knowledge					1116:1124	the knowledge	1112:1124	the knowledge of nitrification characteristics, auto-aggregation performance, and EPS compositions of TN-10 during NaCl shock	1112:1236	These results provided further insights into the knowledge of nitrification characteristics, auto-aggregation performance, and EPS compositions of TN-10 during NaCl shock.
31240650	8	38	used	used	999:1002	arg2	spectrum					986:993	circular dichroism (CD) spectrum	962:993	circular dichroism (CD) spectrum	962:993	Then, circular dichroism (CD) spectrum was used to calculate the contents of each protein secondary structure.
31240650	6	39	theme	substances	770:779	arg1	similar					818:824	similar	818:824	similar	818:824	Extinction-emission matrix (EEM) and Fourier-transform infrared (FT-IR) spectrum analyses showed that the compositions of extracellular polymeric substances (EPS) by TN-10 after NaCl shock were similar.
31240650	6	39	theme	substances	770:779	arg1	compositions					730:741	the compositions	726:741	the compositions of extracellular polymeric substances (EPS) by TN-10 after NaCl shock	726:811	Extinction-emission matrix (EEM) and Fourier-transform infrared (FT-IR) spectrum analyses showed that the compositions of extracellular polymeric substances (EPS) by TN-10 after NaCl shock were similar.
31240650	9	40	theme	compositions	1198:1209	arg1	knowledge					1116:1124	the knowledge	1112:1124	the knowledge of nitrification characteristics, auto-aggregation performance, and EPS compositions of TN-10 during NaCl shock	1112:1236	These results provided further insights into the knowledge of nitrification characteristics, auto-aggregation performance, and EPS compositions of TN-10 during NaCl shock.
31240650	3	41	attach	isolated	144:151	arg2	TN-10					137:141	TN-10	137:141	TN-10	137:141	TN-10, isolated from tannery wastewater, exhibited excellent nitrification ability cultivated in media with NaCl contents ranging from 0 to 35 g/L.
31240650	3	41	attach	isolated	144:151	arg1	wastewater					166:175	tannery wastewater	158:175	tannery wastewater	158:175	TN-10, isolated from tannery wastewater, exhibited excellent nitrification ability cultivated in media with NaCl contents ranging from 0 to 35 g/L.
31240650	7	42	theme	EPS	897:899	arg1	polysaccharides					853:867	polysaccharides	853:867	polysaccharides	853:867	Furthermore, proteins and polysaccharides were the main components of EPS, and the contents were greatly influenced by salinity.
31240650	7	42	theme	EPS	897:899	arg1	proteins					840:847	proteins	840:847	proteins	840:847	Furthermore, proteins and polysaccharides were the main components of EPS, and the contents were greatly influenced by salinity.
31240650	7	42	theme	EPS	897:899	arg1	components					883:892	the main components	874:892	the main components of EPS	874:899	Furthermore, proteins and polysaccharides were the main components of EPS, and the contents were greatly influenced by salinity.
31240650	3	43	theme	nitrification	198:210	arg1	ability					212:218	excellent nitrification ability	188:218	excellent nitrification ability cultivated in media with NaCl contents ranging from 0 to 35 g/L	188:282	TN-10, isolated from tannery wastewater, exhibited excellent nitrification ability cultivated in media with NaCl contents ranging from 0 to 35 g/L.
31240650	9	44	theme	auto-aggregation	1160:1175	arg1	performance					1177:1187	auto-aggregation performance	1160:1187	auto-aggregation performance	1160:1187	These results provided further insights into the knowledge of nitrification characteristics, auto-aggregation performance, and EPS compositions of TN-10 during NaCl shock.
31240650	9	45	theme	nitrification	1129:1141	arg1	characteristics					1143:1157	nitrification characteristics	1129:1157	nitrification characteristics	1129:1157	These results provided further insights into the knowledge of nitrification characteristics, auto-aggregation performance, and EPS compositions of TN-10 during NaCl shock.
31240650	9	46	theme	further	1090:1096	arg1	insights					1098:1105	further insights	1090:1105	further insights into the knowledge of nitrification characteristics, auto-aggregation performance, and EPS compositions of TN-10 during NaCl shock	1090:1236	These results provided further insights into the knowledge of nitrification characteristics, auto-aggregation performance, and EPS compositions of TN-10 during NaCl shock.
31240650	4	47	theme	TN-10	332:336	arg1	activities					318:327	antioxidative enzyme activities	297:327	antioxidative enzyme activities of TN-10 stressed by NaCl	297:353	Analysis of antioxidative enzyme activities of TN-10 stressed by NaCl showed that the stain produced more superoxide dismutase (SOD) and catalase (CAT) to fight against salt stress conditions.
31240650	6	48	theme	matrix	644:649	arg1	EEM					652:654	Extinction-emission matrix (EEM) and Fourier-transform infrared (FT-IR) spectrum analyses	624:712	EEM	652:654	Extinction-emission matrix (EEM) and Fourier-transform infrared (FT-IR) spectrum analyses showed that the compositions of extracellular polymeric substances (EPS) by TN-10 after NaCl shock were similar.
31240650	6	49	theme	FT-IR	689:693	arg1	analyses					705:712	Extinction-emission matrix (EEM) and Fourier-transform infrared (FT-IR) spectrum analyses	624:712	analyses	705:712	Extinction-emission matrix (EEM) and Fourier-transform infrared (FT-IR) spectrum analyses showed that the compositions of extracellular polymeric substances (EPS) by TN-10 after NaCl shock were similar.
31240650	0	50	theme	substance	72:80	arg1	characteristic					82:95	extracellular polymeric substance characteristic	48:95	extracellular polymeric substance characteristic of Klebsiella sp	48:112	Effect of NaCl on nitrification performance and extracellular polymeric substance characteristic of Klebsiella sp.
31240650	6	51	theme	Extinction-emission	624:642	arg1	EEM					652:654	Extinction-emission matrix (EEM) and Fourier-transform infrared (FT-IR) spectrum analyses	624:712	EEM	652:654	Extinction-emission matrix (EEM) and Fourier-transform infrared (FT-IR) spectrum analyses showed that the compositions of extracellular polymeric substances (EPS) by TN-10 after NaCl shock were similar.
31240650	9	52	theme	EPS	1194:1196	arg1	compositions					1198:1209	EPS compositions	1194:1209	EPS compositions	1194:1209	These results provided further insights into the knowledge of nitrification characteristics, auto-aggregation performance, and EPS compositions of TN-10 during NaCl shock.
31240650	0	53	theme	polymeric	62:70	arg1	characteristic					82:95	extracellular polymeric substance characteristic	48:95	extracellular polymeric substance characteristic of Klebsiella sp	48:112	Effect of NaCl on nitrification performance and extracellular polymeric substance characteristic of Klebsiella sp.
31240650	5	54	theme	TN-10	518:522	arg1	index					509:513	the auto-aggregation index	488:513	the auto-aggregation index of TN-10	488:522	Moreover, the auto-aggregation index of TN-10 decreased with the increment of NaCl contents, which is consistent with results of zeta potentials.
31240650	3	55	with	media	234:238	arg1	contents					250:257	NaCl contents	245:257	NaCl contents ranging from 0 to 35 g/L	245:282	TN-10, isolated from tannery wastewater, exhibited excellent nitrification ability cultivated in media with NaCl contents ranging from 0 to 35 g/L.
31240650	6	56	theme	spectrum	696:703	arg1	analyses					705:712	Extinction-emission matrix (EEM) and Fourier-transform infrared (FT-IR) spectrum analyses	624:712	analyses	705:712	Extinction-emission matrix (EEM) and Fourier-transform infrared (FT-IR) spectrum analyses showed that the compositions of extracellular polymeric substances (EPS) by TN-10 after NaCl shock were similar.
31921729	4	0	theme	obese	521:525	arg1	children					537:544	18 obese non-NAFLD children	518:544	18 obese non-NAFLD children	518:544	A total of 58 children and adolescents were enrolled in this study, including 25 obese NAFLD patients, 18 obese non-NAFLD children, and 15 healthy children.
31921729	15	1	theme	other	1877:1881	arg1	acids					1889:1893	other amino acids	1877:1893	other amino acids	1877:1893	Functional annotations demonstrated that several pathways were differentially enriched between groups, including metabolism of other amino acids, replication and repair, folding, sorting, degradation, and glycan biosynthesis and metabolism.
31921729	2	2	theme	resonance	274:282	arg1	MRS					298:300	MRS	298:300	MRS	298:300	Methods: All participants underwent magnetic resonance spectroscopy (MRS) to quantify liver fat content.
31921729	2	2	theme	resonance	274:282	arg1	spectroscopy					284:295	magnetic resonance spectroscopy	265:295	magnetic resonance spectroscopy (MRS)	265:301	Methods: All participants underwent magnetic resonance spectroscopy (MRS) to quantify liver fat content.
31921729	10	3	theme	gut	1169:1171	arg1	microbiota					1173:1182	gut microbiota	1169:1182	gut microbiota	1169:1182	Deep sequencing of gut microbiota revealed high abundance of phylum Proteobacteria (Gammaproteobacteria) in obese NAFLD patients, comparing with the control group.
31921729	11	4	theme	Helicobacter	1387:1398	arg1	pylori					1400:1405	less abundant Helicobacter and Helicobacter pylori	1356:1405	pylori	1400:1405	Overall, obese children without NAFLD had less abundant Helicobacter and Helicobacter pylori.
31921729	16	5	theme	children	2174:2181	arg1	group					2183:2187	the healthy children group	2162:2187	the healthy children group	2162:2187	Conclusion: Significantly differences are observed in gut microbiota composition and functional annotations between obese children with and without NAFLD in comparison to the healthy children group.
31921729	14	6	from	differences	1690:1700	arg1	terms					1705:1709	terms	1705:1709	terms of alpha diversity	1705:1728	There were not significant differences in terms of alpha diversity among three groups.
31921729	13	7	theme	only	1577:1580	arg1	prausnitzii					1557:1567	Faecalibacterium prausnitzii	1540:1567	Faecalibacterium prausnitzii	1540:1567	Faecalibacterium prausnitzii was the only species representing a difference between obese children with and without NAFLD.
31921729	13	7	theme	only	1577:1580	arg1	species					1582:1588	the only species	1573:1588	the only species representing a difference between obese children with and without NAFLD	1573:1660	Faecalibacterium prausnitzii was the only species representing a difference between obese children with and without NAFLD.
31921729	10	8	theme	control	1299:1305	arg1	group					1307:1311	the control group	1295:1311	the control group	1295:1311	Deep sequencing of gut microbiota revealed high abundance of phylum Proteobacteria (Gammaproteobacteria) in obese NAFLD patients, comparing with the control group.
31921729	15	9	theme	Functional	1750:1759	arg1	annotations					1761:1771	Functional annotations	1750:1771	Functional annotations	1750:1771	Functional annotations demonstrated that several pathways were differentially enriched between groups, including metabolism of other amino acids, replication and repair, folding, sorting, degradation, and glycan biosynthesis and metabolism.
31921729	14	10	theme	significant	1678:1688	arg1	differences					1690:1700	significant differences	1678:1700	significant differences in terms of alpha diversity among three groups	1678:1747	There were not significant differences in terms of alpha diversity among three groups.
31921729	13	11	theme	Faecalibacterium	1540:1555	arg1	prausnitzii					1557:1567	Faecalibacterium prausnitzii	1540:1567	Faecalibacterium prausnitzii	1540:1567	Faecalibacterium prausnitzii was the only species representing a difference between obese children with and without NAFLD.
31921729	13	11	theme	Faecalibacterium	1540:1555	arg1	species					1582:1588	the only species	1573:1588	the only species representing a difference between obese children with and without NAFLD	1573:1660	Faecalibacterium prausnitzii was the only species representing a difference between obese children with and without NAFLD.
31921729	1	12	theme	intestinal	104:113	arg1	flora					115:119	the intestinal flora	100:119	the intestinal flora of nonalcoholic fatty liver disease (NAFLD) in Chinese children and adolescents	100:199	Aim: To investigate the intestinal flora of nonalcoholic fatty liver disease (NAFLD) in Chinese children and adolescents using metagenomic approach.
31921729	10	13	theme	phylum	1211:1216	arg1	Gammaproteobacteria					1234:1252	Gammaproteobacteria	1234:1252	Gammaproteobacteria	1234:1252	Deep sequencing of gut microbiota revealed high abundance of phylum Proteobacteria (Gammaproteobacteria) in obese NAFLD patients, comparing with the control group.
31921729	10	13	theme	phylum	1211:1216	arg1	Proteobacteria					1218:1231	phylum Proteobacteria	1211:1231	phylum Proteobacteria (Gammaproteobacteria)	1211:1253	Deep sequencing of gut microbiota revealed high abundance of phylum Proteobacteria (Gammaproteobacteria) in obese NAFLD patients, comparing with the control group.
31921729	0	14	theme	Fatty	59:63	arg1	Disease					71:77	Nonalcoholic Fatty Liver Disease	46:77	Nonalcoholic Fatty Liver Disease	46:77	Metagenome of Gut Microbiota of Children With Nonalcoholic Fatty Liver Disease.
31921729	1	15	theme	fatty	137:141	arg1	NAFLD					158:162	NAFLD	158:162	NAFLD	158:162	Aim: To investigate the intestinal flora of nonalcoholic fatty liver disease (NAFLD) in Chinese children and adolescents using metagenomic approach.
31921729	1	15	theme	fatty	137:141	arg1	disease					149:155	nonalcoholic fatty liver disease	124:155	nonalcoholic fatty liver disease (NAFLD)	124:163	Aim: To investigate the intestinal flora of nonalcoholic fatty liver disease (NAFLD) in Chinese children and adolescents using metagenomic approach.
31921729	17	16	theme	further	2261:2267	arg1	understanding					2269:2281	a further understanding the gut-liver axis	2259:2300	a further understanding the gut-liver axis of pediatric NAFLD in China	2259:2328	The characteristic of gut microbiota in this study may contribute to a further understanding the gut-liver axis of pediatric NAFLD in China.
31921729	9	17	theme	Significant	1002:1012	arg1	differences					1014:1024	Significant differences	1002:1024	Significant differences in gut microbiota composition and functional annotations among three groups of children and adolescents	1002:1128	Results: Significant differences in gut microbiota composition and functional annotations among three groups of children and adolescents have been observed.
31921729	8	18	theme	Kruskal-Wallis	972:985	arg1	test					987:990	Kruskal-Wallis test	972:990	Kruskal-Wallis test	972:990	At last, the differences of gut microbiota composition and functional annotations between obese with and without NAFLD and healthy children were assessed by Kruskal-Wallis test.
31921729	1	19	theme	disease	149:155	arg1	flora					115:119	the intestinal flora	100:119	the intestinal flora of nonalcoholic fatty liver disease (NAFLD) in Chinese children and adolescents	100:199	Aim: To investigate the intestinal flora of nonalcoholic fatty liver disease (NAFLD) in Chinese children and adolescents using metagenomic approach.
31921729	15	20	theme	glycan	1955:1960	arg1	biosynthesis					1962:1973	glycan biosynthesis	1955:1973	glycan biosynthesis	1955:1973	Functional annotations demonstrated that several pathways were differentially enriched between groups, including metabolism of other amino acids, replication and repair, folding, sorting, degradation, and glycan biosynthesis and metabolism.
31921729	9	21	from	differences	1014:1024	arg1	composition					1044:1054	gut microbiota composition	1029:1054	gut microbiota composition	1029:1054	Results: Significant differences in gut microbiota composition and functional annotations among three groups of children and adolescents have been observed.
31921729	9	21	from	differences	1014:1024	arg1	annotations					1071:1081	functional annotations	1060:1081	functional annotations	1060:1081	Results: Significant differences in gut microbiota composition and functional annotations among three groups of children and adolescents have been observed.
31921729	17	22	from	microbiota	2216:2225	arg1	study					2235:2239	this study	2230:2239	this study	2230:2239	The characteristic of gut microbiota in this study may contribute to a further understanding the gut-liver axis of pediatric NAFLD in China.
31921729	9	23	theme	microbiota	1033:1042	arg1	composition					1044:1054	gut microbiota composition	1029:1054	gut microbiota composition	1029:1054	Results: Significant differences in gut microbiota composition and functional annotations among three groups of children and adolescents have been observed.
31921729	3	24	theme	>5	410:411	arg1	%					412:412	MRS proton density fat fraction (MRS-PDFF) >5%	367:412	MRS proton density fat fraction (MRS-PDFF) >5%	367:412	Hepatic steatosis was defined as MRS proton density fat fraction (MRS-PDFF) >5%.
31921729	3	24	theme	>5	410:411	arg1	steatosis					342:350	Hepatic steatosis	334:350	Hepatic steatosis	334:350	Hepatic steatosis was defined as MRS proton density fat fraction (MRS-PDFF) >5%.
31921729	14	25	theme	alpha	1714:1718	arg1	diversity					1720:1728	alpha diversity	1714:1728	alpha diversity	1714:1728	There were not significant differences in terms of alpha diversity among three groups.
31921729	16	26	dep	group	2183:2187	arg1	comparison					2148:2157	comparison	2148:2157	comparison	2148:2157	Conclusion: Significantly differences are observed in gut microbiota composition and functional annotations between obese children with and without NAFLD in comparison to the healthy children group.
31921729	1	27	from	flora	115:119	arg1	children					176:183	Chinese children	168:183	Chinese children	168:183	Aim: To investigate the intestinal flora of nonalcoholic fatty liver disease (NAFLD) in Chinese children and adolescents using metagenomic approach.
31921729	1	27	from	flora	115:119	arg1	adolescents					189:199	adolescents	189:199	adolescents	189:199	Aim: To investigate the intestinal flora of nonalcoholic fatty liver disease (NAFLD) in Chinese children and adolescents using metagenomic approach.
31921729	3	28	theme	MRS	367:369	arg1	fraction					390:397	MRS proton density fat fraction	367:397	MRS proton density fat fraction	367:397	Hepatic steatosis was defined as MRS proton density fat fraction (MRS-PDFF) >5%.
31921729	17	29	theme	pediatric	2305:2313	arg1	NAFLD					2315:2319	pediatric NAFLD	2305:2319	pediatric NAFLD in China	2305:2328	The characteristic of gut microbiota in this study may contribute to a further understanding the gut-liver axis of pediatric NAFLD in China.
31921729	10	30	theme	obese	1258:1262	arg1	patients					1270:1277	obese NAFLD patients	1258:1277	obese NAFLD patients	1258:1277	Deep sequencing of gut microbiota revealed high abundance of phylum Proteobacteria (Gammaproteobacteria) in obese NAFLD patients, comparing with the control group.
31921729	4	31	theme	obese	496:500	arg1	patients					508:515	25 obese NAFLD patients	493:515	25 obese NAFLD patients	493:515	A total of 58 children and adolescents were enrolled in this study, including 25 obese NAFLD patients, 18 obese non-NAFLD children, and 15 healthy children.
31921729	16	32	theme	microbiota	2049:2058	arg1	composition					2060:2070	gut microbiota composition	2045:2070	gut microbiota composition	2045:2070	Conclusion: Significantly differences are observed in gut microbiota composition and functional annotations between obese children with and without NAFLD in comparison to the healthy children group.
31921729	0	33	theme	Gut	14:16	arg1	Microbiota					18:27	Gut Microbiota	14:27	Gut Microbiota of Children With Nonalcoholic Fatty Liver Disease	14:77	Metagenome of Gut Microbiota of Children With Nonalcoholic Fatty Liver Disease.
31921729	8	34	theme	microbiota	847:856	arg1	composition					858:868	gut microbiota composition	843:868	gut microbiota composition	843:868	At last, the differences of gut microbiota composition and functional annotations between obese with and without NAFLD and healthy children were assessed by Kruskal-Wallis test.
31921729	7	35	theme	sum	721:723	arg1	test					725:728	Wilcoxon rank sum test	707:728	Wilcoxon rank sum test	707:728	Wilcoxon rank sum test and Kruskal-Wallis test were performed to evaluate alpha diversities between groups.
31921729	12	36	theme	Bacteroidetes	1486:1498	arg1	abundance					1473:1481	the abundance	1469:1481	the abundance of Bacteroidetes (Alistipes)	1469:1510	Compared to the control group, in obese children with NAFLD, the abundance of Bacteroidetes (Alistipes) were significantly reduced.
31921729	3	37	theme	density	378:384	arg1	fraction					390:397	MRS proton density fat fraction	367:397	MRS proton density fat fraction	367:397	Hepatic steatosis was defined as MRS proton density fat fraction (MRS-PDFF) >5%.
31921729	6	38	theme	microbiota	695:704	arg1	diversities					676:686	the alpha diversities	666:686	the alpha diversities of gut microbiota	666:704	We used Shannon index to reflect the alpha diversities of gut microbiota.
31921729	7	39	theme	Wilcoxon	707:714	arg1	test					725:728	Wilcoxon rank sum test	707:728	Wilcoxon rank sum test	707:728	Wilcoxon rank sum test and Kruskal-Wallis test were performed to evaluate alpha diversities between groups.
31921729	8	40	theme	annotations	885:895	arg1	differences					828:838	the differences	824:838	the differences of gut microbiota composition and functional annotations between obese with and without NAFLD and healthy children	824:953	At last, the differences of gut microbiota composition and functional annotations between obese with and without NAFLD and healthy children were assessed by Kruskal-Wallis test.
31921729	16	41	theme	obese	2107:2111	arg1	children					2113:2120	obese children	2107:2120	obese children with and without NAFLD in comparison to the healthy children group	2107:2187	Conclusion: Significantly differences are observed in gut microbiota composition and functional annotations between obese children with and without NAFLD in comparison to the healthy children group.
31921729	2	42	theme	liver	315:319	arg1	content					325:331	liver fat content	315:331	liver fat content	315:331	Methods: All participants underwent magnetic resonance spectroscopy (MRS) to quantify liver fat content.
31921729	11	43	theme	abundant	1361:1368	arg1	Helicobacter					1370:1381	less abundant Helicobacter and Helicobacter pylori	1356:1405	Helicobacter	1370:1381	Overall, obese children without NAFLD had less abundant Helicobacter and Helicobacter pylori.
31921729	6	44	theme	alpha	670:674	arg1	diversities					676:686	the alpha diversities	666:686	the alpha diversities of gut microbiota	666:704	We used Shannon index to reflect the alpha diversities of gut microbiota.
31921729	10	45	theme	Deep	1150:1153	arg1	sequencing					1155:1164	Deep sequencing	1150:1164	Deep sequencing of gut microbiota	1150:1182	Deep sequencing of gut microbiota revealed high abundance of phylum Proteobacteria (Gammaproteobacteria) in obese NAFLD patients, comparing with the control group.
31921729	10	46	theme	high	1193:1196	arg1	abundance					1198:1206	high abundance	1193:1206	high abundance of phylum Proteobacteria (Gammaproteobacteria) in obese NAFLD patients	1193:1277	Deep sequencing of gut microbiota revealed high abundance of phylum Proteobacteria (Gammaproteobacteria) in obese NAFLD patients, comparing with the control group.
31921729	12	47	theme	obese	1442:1446	arg1	children					1448:1455	obese children	1442:1455	obese children with NAFLD	1442:1466	Compared to the control group, in obese children with NAFLD, the abundance of Bacteroidetes (Alistipes) were significantly reduced.
31921729	8	48	theme	NAFLD	928:932	arg1	children					946:953	NAFLD and healthy children	928:953	NAFLD and healthy children	928:953	At last, the differences of gut microbiota composition and functional annotations between obese with and without NAFLD and healthy children were assessed by Kruskal-Wallis test.
31921729	17	49	from	China	2324:2328	arg1	understanding					2269:2281	a further understanding the gut-liver axis	2259:2300	a further understanding the gut-liver axis of pediatric NAFLD in China	2259:2328	The characteristic of gut microbiota in this study may contribute to a further understanding the gut-liver axis of pediatric NAFLD in China.
31921729	10	50	theme	microbiota	1173:1182	arg1	sequencing					1155:1164	Deep sequencing	1150:1164	Deep sequencing of gut microbiota	1150:1182	Deep sequencing of gut microbiota revealed high abundance of phylum Proteobacteria (Gammaproteobacteria) in obese NAFLD patients, comparing with the control group.
31921729	8	51	theme	healthy	938:944	arg1	children					946:953	NAFLD and healthy children	928:953	NAFLD and healthy children	928:953	At last, the differences of gut microbiota composition and functional annotations between obese with and without NAFLD and healthy children were assessed by Kruskal-Wallis test.
31921729	10	52	from	abundance	1198:1206	arg1	patients					1270:1277	obese NAFLD patients	1258:1277	obese NAFLD patients	1258:1277	Deep sequencing of gut microbiota revealed high abundance of phylum Proteobacteria (Gammaproteobacteria) in obese NAFLD patients, comparing with the control group.
31921729	4	53	theme	non-NAFLD	527:535	arg1	children					537:544	18 obese non-NAFLD children	518:544	18 obese non-NAFLD children	518:544	A total of 58 children and adolescents were enrolled in this study, including 25 obese NAFLD patients, 18 obese non-NAFLD children, and 15 healthy children.
31921729	17	54	from	NAFLD	2315:2319	arg1	China					2324:2328	China	2324:2328	China	2324:2328	The characteristic of gut microbiota in this study may contribute to a further understanding the gut-liver axis of pediatric NAFLD in China.
31921729	2	55	theme	magnetic	265:272	arg1	MRS					298:300	MRS	298:300	MRS	298:300	Methods: All participants underwent magnetic resonance spectroscopy (MRS) to quantify liver fat content.
31921729	2	55	theme	magnetic	265:272	arg1	spectroscopy					284:295	magnetic resonance spectroscopy	265:295	magnetic resonance spectroscopy (MRS)	265:301	Methods: All participants underwent magnetic resonance spectroscopy (MRS) to quantify liver fat content.
31921729	12	56	theme	control	1424:1430	arg1	group					1432:1436	the control group	1420:1436	the control group	1420:1436	Compared to the control group, in obese children with NAFLD, the abundance of Bacteroidetes (Alistipes) were significantly reduced.
31921729	16	57	located	observed	2033:2040	arg1	composition					2060:2070	gut microbiota composition	2045:2070	gut microbiota composition	2045:2070	Conclusion: Significantly differences are observed in gut microbiota composition and functional annotations between obese children with and without NAFLD in comparison to the healthy children group.
31921729	16	57	located	observed	2033:2040	arg1	annotations					2087:2097	functional annotations	2076:2097	functional annotations	2076:2097	Conclusion: Significantly differences are observed in gut microbiota composition and functional annotations between obese children with and without NAFLD in comparison to the healthy children group.
31921729	16	57	located	observed	2033:2040	arg2	differences					2017:2027	differences	2017:2027	differences	2017:2027	Conclusion: Significantly differences are observed in gut microbiota composition and functional annotations between obese children with and without NAFLD in comparison to the healthy children group.
31921729	16	58	theme	healthy	2166:2172	arg1	group					2183:2187	the healthy children group	2162:2187	the healthy children group	2162:2187	Conclusion: Significantly differences are observed in gut microbiota composition and functional annotations between obese children with and without NAFLD in comparison to the healthy children group.
31921729	5	59	theme	Stool	572:576	arg1	samples					578:584	Stool samples	572:584	Stool samples	572:584	Stool samples were collected and analyzed with metagenomics.
31921729	16	60	theme	functional	2076:2085	arg1	annotations					2087:2097	functional annotations	2076:2097	functional annotations	2076:2097	Conclusion: Significantly differences are observed in gut microbiota composition and functional annotations between obese children with and without NAFLD in comparison to the healthy children group.
31921729	17	61	dep	understanding	2269:2281	arg1	axis					2297:2300	the gut-liver axis	2283:2300	a further understanding the gut-liver axis of pediatric NAFLD in China	2259:2328	The characteristic of gut microbiota in this study may contribute to a further understanding the gut-liver axis of pediatric NAFLD in China.
31921729	11	62	contain	had	1352:1354	arg1	children					1329:1336	obese children	1323:1336	obese children without NAFLD	1323:1350	Overall, obese children without NAFLD had less abundant Helicobacter and Helicobacter pylori.
31921729	11	62	contain	had	1352:1354	arg2	pylori					1400:1405	less abundant Helicobacter and Helicobacter pylori	1356:1405	pylori	1400:1405	Overall, obese children without NAFLD had less abundant Helicobacter and Helicobacter pylori.
31921729	11	62	contain	had	1352:1354	arg2	Helicobacter					1370:1381	less abundant Helicobacter and Helicobacter pylori	1356:1405	Helicobacter	1370:1381	Overall, obese children without NAFLD had less abundant Helicobacter and Helicobacter pylori.
31921729	14	63	theme	diversity	1720:1728	arg1	terms					1705:1709	terms	1705:1709	terms of alpha diversity	1705:1728	There were not significant differences in terms of alpha diversity among three groups.
31921729	7	64	theme	alpha	781:785	arg1	diversities					787:797	alpha diversities	781:797	alpha diversities between groups	781:812	Wilcoxon rank sum test and Kruskal-Wallis test were performed to evaluate alpha diversities between groups.
31921729	1	65	theme	Chinese	168:174	arg1	children					176:183	Chinese children	168:183	Chinese children	168:183	Aim: To investigate the intestinal flora of nonalcoholic fatty liver disease (NAFLD) in Chinese children and adolescents using metagenomic approach.
31921729	6	66	theme	gut	691:693	arg1	microbiota					695:704	gut microbiota	691:704	gut microbiota	691:704	We used Shannon index to reflect the alpha diversities of gut microbiota.
31921729	17	67	theme	NAFLD	2315:2319	arg1	understanding					2269:2281	a further understanding the gut-liver axis	2259:2300	a further understanding the gut-liver axis of pediatric NAFLD in China	2259:2328	The characteristic of gut microbiota in this study may contribute to a further understanding the gut-liver axis of pediatric NAFLD in China.
31921729	17	68	from	understanding	2269:2281	arg1	China					2324:2328	China	2324:2328	China	2324:2328	The characteristic of gut microbiota in this study may contribute to a further understanding the gut-liver axis of pediatric NAFLD in China.
31921729	11	69	theme	obese	1323:1327	arg1	children					1329:1336	obese children	1323:1336	obese children without NAFLD	1323:1350	Overall, obese children without NAFLD had less abundant Helicobacter and Helicobacter pylori.
31921729	10	70	theme	Proteobacteria	1218:1231	arg1	abundance					1198:1206	high abundance	1193:1206	high abundance of phylum Proteobacteria (Gammaproteobacteria) in obese NAFLD patients	1193:1277	Deep sequencing of gut microbiota revealed high abundance of phylum Proteobacteria (Gammaproteobacteria) in obese NAFLD patients, comparing with the control group.
31921729	15	71	theme	several	1791:1797	arg1	pathways					1799:1806	several pathways	1791:1806	several pathways	1791:1806	Functional annotations demonstrated that several pathways were differentially enriched between groups, including metabolism of other amino acids, replication and repair, folding, sorting, degradation, and glycan biosynthesis and metabolism.
31921729	17	72	theme	gut	2212:2214	arg1	microbiota					2216:2225	gut microbiota	2212:2225	gut microbiota in this study	2212:2239	The characteristic of gut microbiota in this study may contribute to a further understanding the gut-liver axis of pediatric NAFLD in China.
31921729	17	73	from	study	2235:2239	arg1	characteristic					2194:2207	The characteristic	2190:2207	The characteristic of gut microbiota in this study	2190:2239	The characteristic of gut microbiota in this study may contribute to a further understanding the gut-liver axis of pediatric NAFLD in China.
31921729	0	74	theme	Liver	65:69	arg1	Disease					71:77	Nonalcoholic Fatty Liver Disease	46:77	Nonalcoholic Fatty Liver Disease	46:77	Metagenome of Gut Microbiota of Children With Nonalcoholic Fatty Liver Disease.
31921729	1	75	theme	nonalcoholic	124:135	arg1	NAFLD					158:162	NAFLD	158:162	NAFLD	158:162	Aim: To investigate the intestinal flora of nonalcoholic fatty liver disease (NAFLD) in Chinese children and adolescents using metagenomic approach.
31921729	1	75	theme	nonalcoholic	124:135	arg1	disease					149:155	nonalcoholic fatty liver disease	124:155	nonalcoholic fatty liver disease (NAFLD)	124:163	Aim: To investigate the intestinal flora of nonalcoholic fatty liver disease (NAFLD) in Chinese children and adolescents using metagenomic approach.
31921729	1	76	theme	liver	143:147	arg1	NAFLD					158:162	NAFLD	158:162	NAFLD	158:162	Aim: To investigate the intestinal flora of nonalcoholic fatty liver disease (NAFLD) in Chinese children and adolescents using metagenomic approach.
31921729	1	76	theme	liver	143:147	arg1	disease					149:155	nonalcoholic fatty liver disease	124:155	nonalcoholic fatty liver disease (NAFLD)	124:163	Aim: To investigate the intestinal flora of nonalcoholic fatty liver disease (NAFLD) in Chinese children and adolescents using metagenomic approach.
31921729	17	77	theme	gut-liver	2287:2295	arg1	axis					2297:2300	the gut-liver axis	2283:2300	a further understanding the gut-liver axis of pediatric NAFLD in China	2259:2328	The characteristic of gut microbiota in this study may contribute to a further understanding the gut-liver axis of pediatric NAFLD in China.
31921729	4	78	theme	children	429:436	arg1	total					417:421	A total	415:421	A total of 58 children and adolescents	415:452	A total of 58 children and adolescents were enrolled in this study, including 25 obese NAFLD patients, 18 obese non-NAFLD children, and 15 healthy children.
31921729	17	79	theme	microbiota	2216:2225	arg1	characteristic					2194:2207	The characteristic	2190:2207	The characteristic of gut microbiota in this study	2190:2239	The characteristic of gut microbiota in this study may contribute to a further understanding the gut-liver axis of pediatric NAFLD in China.
31921729	9	80	theme	gut	1029:1031	arg1	composition					1044:1054	gut microbiota composition	1029:1054	gut microbiota composition	1029:1054	Results: Significant differences in gut microbiota composition and functional annotations among three groups of children and adolescents have been observed.
31921729	17	81	from	characteristic	2194:2207	arg1	study					2235:2239	this study	2230:2239	this study	2230:2239	The characteristic of gut microbiota in this study may contribute to a further understanding the gut-liver axis of pediatric NAFLD in China.
31921729	12	82	with	children	1448:1455	arg1	NAFLD					1462:1466	NAFLD	1462:1466	NAFLD	1462:1466	Compared to the control group, in obese children with NAFLD, the abundance of Bacteroidetes (Alistipes) were significantly reduced.
31921729	4	83	theme	adolescents	442:452	arg1	total					417:421	A total	415:421	A total of 58 children and adolescents	415:452	A total of 58 children and adolescents were enrolled in this study, including 25 obese NAFLD patients, 18 obese non-NAFLD children, and 15 healthy children.
31921729	1	84	theme	metagenomic	207:217	arg1	approach					219:226	metagenomic approach	207:226	metagenomic approach	207:226	Aim: To investigate the intestinal flora of nonalcoholic fatty liver disease (NAFLD) in Chinese children and adolescents using metagenomic approach.
31921729	6	85	theme	Shannon	641:647	arg1	index					649:653	Shannon index	641:653	Shannon index	641:653	We used Shannon index to reflect the alpha diversities of gut microbiota.
31921729	15	86	theme	amino	1883:1887	arg1	acids					1889:1893	other amino acids	1877:1893	other amino acids	1877:1893	Functional annotations demonstrated that several pathways were differentially enriched between groups, including metabolism of other amino acids, replication and repair, folding, sorting, degradation, and glycan biosynthesis and metabolism.
31921729	6	87	used	used	636:639	arg2	We					633:634	We	633:634	We	633:634	We used Shannon index to reflect the alpha diversities of gut microbiota.
31921729	3	88	theme	proton	371:376	arg1	fraction					390:397	MRS proton density fat fraction	367:397	MRS proton density fat fraction	367:397	Hepatic steatosis was defined as MRS proton density fat fraction (MRS-PDFF) >5%.
31921729	4	89	theme	NAFLD	502:506	arg1	patients					508:515	25 obese NAFLD patients	493:515	25 obese NAFLD patients	493:515	A total of 58 children and adolescents were enrolled in this study, including 25 obese NAFLD patients, 18 obese non-NAFLD children, and 15 healthy children.
31921729	9	90	theme	functional	1060:1069	arg1	annotations					1071:1081	functional annotations	1060:1081	functional annotations	1060:1081	Results: Significant differences in gut microbiota composition and functional annotations among three groups of children and adolescents have been observed.
31921729	0	91	theme	Microbiota	18:27	arg1	Metagenome					0:9	Metagenome	0:9	Metagenome of Gut Microbiota of Children With Nonalcoholic Fatty Liver Disease.	0:78	Metagenome of Gut Microbiota of Children With Nonalcoholic Fatty Liver Disease.
31921729	16	92	theme	gut	2045:2047	arg1	composition					2060:2070	gut microbiota composition	2045:2070	gut microbiota composition	2045:2070	Conclusion: Significantly differences are observed in gut microbiota composition and functional annotations between obese children with and without NAFLD in comparison to the healthy children group.
31921729	0	93	theme	Children	32:39	arg1	Microbiota					18:27	Gut Microbiota	14:27	Gut Microbiota of Children With Nonalcoholic Fatty Liver Disease	14:77	Metagenome of Gut Microbiota of Children With Nonalcoholic Fatty Liver Disease.
31921729	7	94	theme	Kruskal-Wallis	734:747	arg1	test					749:752	Kruskal-Wallis test	734:752	Kruskal-Wallis test	734:752	Wilcoxon rank sum test and Kruskal-Wallis test were performed to evaluate alpha diversities between groups.
31921729	8	95	theme	gut	843:845	arg1	composition					858:868	gut microbiota composition	843:868	gut microbiota composition	843:868	At last, the differences of gut microbiota composition and functional annotations between obese with and without NAFLD and healthy children were assessed by Kruskal-Wallis test.
31921729	3	96	theme	Hepatic	334:340	arg1	%					412:412	MRS proton density fat fraction (MRS-PDFF) >5%	367:412	MRS proton density fat fraction (MRS-PDFF) >5%	367:412	Hepatic steatosis was defined as MRS proton density fat fraction (MRS-PDFF) >5%.
31921729	3	96	theme	Hepatic	334:340	arg1	steatosis					342:350	Hepatic steatosis	334:350	Hepatic steatosis	334:350	Hepatic steatosis was defined as MRS proton density fat fraction (MRS-PDFF) >5%.
31921729	0	97	theme	Nonalcoholic	46:57	arg1	Disease					71:77	Nonalcoholic Fatty Liver Disease	46:77	Nonalcoholic Fatty Liver Disease	46:77	Metagenome of Gut Microbiota of Children With Nonalcoholic Fatty Liver Disease.
31921729	3	98	dep	>5	410:411	arg1	fraction					390:397	MRS proton density fat fraction	367:397	MRS proton density fat fraction	367:397	Hepatic steatosis was defined as MRS proton density fat fraction (MRS-PDFF) >5%.
31921729	8	99	theme	composition	858:868	arg1	differences					828:838	the differences	824:838	the differences of gut microbiota composition and functional annotations between obese with and without NAFLD and healthy children	824:953	At last, the differences of gut microbiota composition and functional annotations between obese with and without NAFLD and healthy children were assessed by Kruskal-Wallis test.
31921729	3	100	theme	fat	386:388	arg1	fraction					390:397	MRS proton density fat fraction	367:397	MRS proton density fat fraction	367:397	Hepatic steatosis was defined as MRS proton density fat fraction (MRS-PDFF) >5%.
31921729	13	101	theme	obese	1624:1628	arg1	children					1630:1637	obese children	1624:1637	obese children with and without NAFLD	1624:1660	Faecalibacterium prausnitzii was the only species representing a difference between obese children with and without NAFLD.
31921729	9	102	theme	children	1105:1112	arg1	children					1105:1112	children	1105:1112	children	1105:1112	Results: Significant differences in gut microbiota composition and functional annotations among three groups of children and adolescents have been observed.
31921729	9	102	theme	children	1105:1112	arg1	groups					1095:1100	three groups	1089:1100	three groups of children and adolescents	1089:1128	Results: Significant differences in gut microbiota composition and functional annotations among three groups of children and adolescents have been observed.
31921729	9	102	theme	children	1105:1112	arg1	adolescents					1118:1128	adolescents	1118:1128	adolescents	1118:1128	Results: Significant differences in gut microbiota composition and functional annotations among three groups of children and adolescents have been observed.
31921729	2	103	theme	fat	321:323	arg1	content					325:331	liver fat content	315:331	liver fat content	315:331	Methods: All participants underwent magnetic resonance spectroscopy (MRS) to quantify liver fat content.
31921729	7	104	theme	rank	716:719	arg1	test					725:728	Wilcoxon rank sum test	707:728	Wilcoxon rank sum test	707:728	Wilcoxon rank sum test and Kruskal-Wallis test were performed to evaluate alpha diversities between groups.
31921729	8	105	theme	functional	874:883	arg1	annotations					885:895	functional annotations	874:895	functional annotations	874:895	At last, the differences of gut microbiota composition and functional annotations between obese with and without NAFLD and healthy children were assessed by Kruskal-Wallis test.
31921729	9	106	theme	adolescents	1118:1128	arg1	children					1105:1112	children	1105:1112	children	1105:1112	Results: Significant differences in gut microbiota composition and functional annotations among three groups of children and adolescents have been observed.
31921729	9	106	theme	adolescents	1118:1128	arg1	groups					1095:1100	three groups	1089:1100	three groups of children and adolescents	1089:1128	Results: Significant differences in gut microbiota composition and functional annotations among three groups of children and adolescents have been observed.
31921729	9	106	theme	adolescents	1118:1128	arg1	adolescents					1118:1128	adolescents	1118:1128	adolescents	1118:1128	Results: Significant differences in gut microbiota composition and functional annotations among three groups of children and adolescents have been observed.
31921729	10	107	theme	NAFLD	1264:1268	arg1	patients					1270:1277	obese NAFLD patients	1258:1277	obese NAFLD patients	1258:1277	Deep sequencing of gut microbiota revealed high abundance of phylum Proteobacteria (Gammaproteobacteria) in obese NAFLD patients, comparing with the control group.
31921729	4	108	theme	healthy	554:560	arg1	children					562:569	15 healthy children	551:569	15 healthy children	551:569	A total of 58 children and adolescents were enrolled in this study, including 25 obese NAFLD patients, 18 obese non-NAFLD children, and 15 healthy children.
31921729	15	109	theme	acids	1889:1893	arg1	folding					1920:1926	folding	1920:1926	folding	1920:1926	Functional annotations demonstrated that several pathways were differentially enriched between groups, including metabolism of other amino acids, replication and repair, folding, sorting, degradation, and glycan biosynthesis and metabolism.
31921729	15	109	theme	acids	1889:1893	arg1	sorting					1929:1935	sorting	1929:1935	sorting	1929:1935	Functional annotations demonstrated that several pathways were differentially enriched between groups, including metabolism of other amino acids, replication and repair, folding, sorting, degradation, and glycan biosynthesis and metabolism.
31921729	15	109	theme	acids	1889:1893	arg1	biosynthesis					1962:1973	glycan biosynthesis	1955:1973	glycan biosynthesis	1955:1973	Functional annotations demonstrated that several pathways were differentially enriched between groups, including metabolism of other amino acids, replication and repair, folding, sorting, degradation, and glycan biosynthesis and metabolism.
31921729	15	109	theme	acids	1889:1893	arg1	metabolism					1863:1872	metabolism	1863:1872	metabolism of other amino acids	1863:1893	Functional annotations demonstrated that several pathways were differentially enriched between groups, including metabolism of other amino acids, replication and repair, folding, sorting, degradation, and glycan biosynthesis and metabolism.
31921729	15	109	theme	acids	1889:1893	arg1	replication					1896:1906	replication	1896:1906	replication	1896:1906	Functional annotations demonstrated that several pathways were differentially enriched between groups, including metabolism of other amino acids, replication and repair, folding, sorting, degradation, and glycan biosynthesis and metabolism.
31921729	15	109	theme	acids	1889:1893	arg1	metabolism					1979:1988	metabolism	1979:1988	metabolism	1979:1988	Functional annotations demonstrated that several pathways were differentially enriched between groups, including metabolism of other amino acids, replication and repair, folding, sorting, degradation, and glycan biosynthesis and metabolism.
31921729	15	109	theme	acids	1889:1893	arg1	repair					1912:1917	repair	1912:1917	repair	1912:1917	Functional annotations demonstrated that several pathways were differentially enriched between groups, including metabolism of other amino acids, replication and repair, folding, sorting, degradation, and glycan biosynthesis and metabolism.
31921729	15	109	theme	acids	1889:1893	arg1	degradation					1938:1948	degradation	1938:1948	degradation	1938:1948	Functional annotations demonstrated that several pathways were differentially enriched between groups, including metabolism of other amino acids, replication and repair, folding, sorting, degradation, and glycan biosynthesis and metabolism.
31921729	0	110	with	Children	32:39	arg1	Disease					71:77	Nonalcoholic Fatty Liver Disease	46:77	Nonalcoholic Fatty Liver Disease	46:77	Metagenome of Gut Microbiota of Children With Nonalcoholic Fatty Liver Disease.
29922272	12	0	theme	obesity	1874:1880	arg1	markers					1892:1898	novel obesity bacterial markers	1868:1898	novel obesity bacterial markers	1868:1898	The assignment of novel obesity bacterial markers may open avenues for the development of patient-tailored treatments dependent on age-related microbiota profiles.
29922272	10	1	theme	bile	1616:1619	arg1	acid					1621:1624	secondary bile acid	1606:1624	secondary bile acid	1606:1624	Particularly, biosynthesis of primary bile acid and steroid acids, metabolism of fructose, mannose, galactose, butanoate, and pentose phosphate and glycolysis/gluconeogenesis were for the majority associated to obese, while biosynthesis and metabolism of glycan, biosynthesis of secondary bile acid, metabolism of steroid hormone and lipoic acid were associated to NW adolescents.
29922272	10	2	theme	phosphate	1461:1469	arg1	glycolysis/gluconeogenesis					1475:1500	glycolysis/gluconeogenesis	1475:1500	glycolysis/gluconeogenesis	1475:1500	Particularly, biosynthesis of primary bile acid and steroid acids, metabolism of fructose, mannose, galactose, butanoate, and pentose phosphate and glycolysis/gluconeogenesis were for the majority associated to obese, while biosynthesis and metabolism of glycan, biosynthesis of secondary bile acid, metabolism of steroid hormone and lipoic acid were associated to NW adolescents.
29922272	10	2	theme	phosphate	1461:1469	arg1	biosynthesis					1341:1352	biosynthesis	1341:1352	biosynthesis of primary bile acid and steroid acids	1341:1391	Particularly, biosynthesis of primary bile acid and steroid acids, metabolism of fructose, mannose, galactose, butanoate, and pentose phosphate and glycolysis/gluconeogenesis were for the majority associated to obese, while biosynthesis and metabolism of glycan, biosynthesis of secondary bile acid, metabolism of steroid hormone and lipoic acid were associated to NW adolescents.
29922272	10	2	theme	phosphate	1461:1469	arg1	metabolism					1394:1403	metabolism	1394:1403	metabolism of fructose, mannose, galactose, butanoate, and pentose phosphate	1394:1469	Particularly, biosynthesis of primary bile acid and steroid acids, metabolism of fructose, mannose, galactose, butanoate, and pentose phosphate and glycolysis/gluconeogenesis were for the majority associated to obese, while biosynthesis and metabolism of glycan, biosynthesis of secondary bile acid, metabolism of steroid hormone and lipoic acid were associated to NW adolescents.
29922272	7	3	theme	microbiota	911:920	arg1	profiles					922:929	resultant microbiota profiles	901:929	resultant microbiota profiles	901:929	Ecological analyses revealed a dissimilarity among the subgroups, and resultant microbiota profiles differed between obese adolescents and adults.
29922272	10	4	theme	hormone	1649:1655	arg1	biosynthesis					1590:1601	biosynthesis	1590:1601	biosynthesis of secondary bile acid	1590:1624	Particularly, biosynthesis of primary bile acid and steroid acids, metabolism of fructose, mannose, galactose, butanoate, and pentose phosphate and glycolysis/gluconeogenesis were for the majority associated to obese, while biosynthesis and metabolism of glycan, biosynthesis of secondary bile acid, metabolism of steroid hormone and lipoic acid were associated to NW adolescents.
29922272	10	4	theme	hormone	1649:1655	arg1	metabolism					1627:1636	metabolism	1627:1636	metabolism of steroid hormone	1627:1655	Particularly, biosynthesis of primary bile acid and steroid acids, metabolism of fructose, mannose, galactose, butanoate, and pentose phosphate and glycolysis/gluconeogenesis were for the majority associated to obese, while biosynthesis and metabolism of glycan, biosynthesis of secondary bile acid, metabolism of steroid hormone and lipoic acid were associated to NW adolescents.
29922272	10	4	theme	hormone	1649:1655	arg1	acid					1668:1671	lipoic acid	1661:1671	lipoic acid	1661:1671	Particularly, biosynthesis of primary bile acid and steroid acids, metabolism of fructose, mannose, galactose, butanoate, and pentose phosphate and glycolysis/gluconeogenesis were for the majority associated to obese, while biosynthesis and metabolism of glycan, biosynthesis of secondary bile acid, metabolism of steroid hormone and lipoic acid were associated to NW adolescents.
29922272	10	4	theme	hormone	1649:1655	arg1	metabolism					1568:1577	metabolism	1568:1577	metabolism	1568:1577	Particularly, biosynthesis of primary bile acid and steroid acids, metabolism of fructose, mannose, galactose, butanoate, and pentose phosphate and glycolysis/gluconeogenesis were for the majority associated to obese, while biosynthesis and metabolism of glycan, biosynthesis of secondary bile acid, metabolism of steroid hormone and lipoic acid were associated to NW adolescents.
29922272	10	4	theme	hormone	1649:1655	arg1	biosynthesis					1551:1562	biosynthesis	1551:1562	biosynthesis	1551:1562	Particularly, biosynthesis of primary bile acid and steroid acids, metabolism of fructose, mannose, galactose, butanoate, and pentose phosphate and glycolysis/gluconeogenesis were for the majority associated to obese, while biosynthesis and metabolism of glycan, biosynthesis of secondary bile acid, metabolism of steroid hormone and lipoic acid were associated to NW adolescents.
29922272	11	5	theme	gut	1746:1748	arg1	microbiota					1750:1759	gut microbiota	1746:1759	gut microbiota	1746:1759	Our study revealed unique features of gut microbiota in terms of ecological patterns, microbial composition and metabolism in obese patients.
29922272	5	6	theme	correlation	685:695	arg1	analyses					697:704	correlation analyses	685:704	correlation analyses	685:704	Ecological representations of microbial communities were computed, and univariate, multivariate, and correlation analyses performed on bacterial profiles.
29922272	8	7	theme	NW	1237:1238	arg1	adolescents					1240:1250	NW adolescents	1237:1250	NW adolescents	1237:1250	Using statistical analyses, we assigned, as microbial markers, Faecalibacterium prausnitzii and Actinomyces to the microbiota of obese adolescents, and Parabacteroides, Rikenellaceae, Bacteroides caccae, Barnesiellaceae, and Oscillospira to the microbiota of NW adolescents.
29922272	6	8	from	prediction	743:752	arg1	surveys					806:812	16S rRNA gene surveys	792:812	16S rRNA gene surveys	792:812	The prediction of metagenome functional content from 16S rRNA gene surveys was carried out.
29922272	3	9	dep	investigated	305:316	arg1	compared					384:391	compared	384:391	compared to age-matched normal weight (NW) volunteers in order to assemble age- and obesity-related microbiota profiles	384:502	We investigated the composition of gut microbiota in obese adolescents and adults compared to age-matched normal weight (NW) volunteers in order to assemble age- and obesity-related microbiota profiles.
29922272	9	10	theme	metabolic	1267:1275	arg1	profiles					1277:1284	The predicted metabolic profiles	1253:1284	The predicted metabolic profiles	1253:1284	The predicted metabolic profiles resulted different in adolescent groups.
29922272	1	11	theme	few	182:184	arg1	decades					186:192	the last few decades	173:192	the last few decades	173:192	Obesity levels, especially in children, have dramatically increased over the last few decades.
29922272	11	12	from	patients	1840:1847	arg1	terms					1764:1768	terms	1764:1768	terms of ecological patterns, microbial composition and metabolism in obese patients	1764:1847	Our study revealed unique features of gut microbiota in terms of ecological patterns, microbial composition and metabolism in obese patients.
29922272	10	13	theme	lipoic	1661:1666	arg1	acid					1668:1671	lipoic acid	1661:1671	lipoic acid	1661:1671	Particularly, biosynthesis of primary bile acid and steroid acids, metabolism of fructose, mannose, galactose, butanoate, and pentose phosphate and glycolysis/gluconeogenesis were for the majority associated to obese, while biosynthesis and metabolism of glycan, biosynthesis of secondary bile acid, metabolism of steroid hormone and lipoic acid were associated to NW adolescents.
29922272	2	14	theme	obesity	293:299	arg1	pathophysiology					274:288	the pathophysiology	270:288	the pathophysiology of obesity	270:299	Recently, several studies highlighted the involvement of gut microbiota in the pathophysiology of obesity.
29922272	2	15	theme	several	205:211	arg1	studies					213:219	several studies	205:219	several studies	205:219	Recently, several studies highlighted the involvement of gut microbiota in the pathophysiology of obesity.
29922272	5	16	theme	bacterial	719:727	arg1	profiles					729:736	bacterial profiles	719:736	bacterial profiles	719:736	Ecological representations of microbial communities were computed, and univariate, multivariate, and correlation analyses performed on bacterial profiles.
29922272	2	17	theme	gut	252:254	arg1	microbiota					256:265	gut microbiota	252:265	gut microbiota	252:265	Recently, several studies highlighted the involvement of gut microbiota in the pathophysiology of obesity.
29922272	11	18	from	patterns	1784:1791	arg1	patients					1840:1847	obese patients	1834:1847	obese patients	1834:1847	Our study revealed unique features of gut microbiota in terms of ecological patterns, microbial composition and metabolism in obese patients.
29922272	0	19	from	Markers	15:21	arg1	Adolescent					32:41	Adolescent	32:41	Adolescent	32:41	Gut Microbiota Markers in Obese Adolescent and Adult Patients: Age-Dependent Differential Patterns.
29922272	0	19	from	Markers	15:21	arg1	Adult					47:51	Adult	47:51	Adult	47:51	Gut Microbiota Markers in Obese Adolescent and Adult Patients: Age-Dependent Differential Patterns.
29922272	0	20	theme	Age-Dependent	63:75	arg1	Patterns					90:97	Age-Dependent Differential Patterns	63:97	Age-Dependent Differential Patterns	63:97	Gut Microbiota Markers in Obese Adolescent and Adult Patients: Age-Dependent Differential Patterns.
29922272	5	21	theme	microbial	614:622	arg1	communities					624:634	microbial communities	614:634	microbial communities	614:634	Ecological representations of microbial communities were computed, and univariate, multivariate, and correlation analyses performed on bacterial profiles.
29922272	3	22	theme	obese	355:359	arg1	adolescents					361:371	obese adolescents	355:371	obese adolescents	355:371	We investigated the composition of gut microbiota in obese adolescents and adults compared to age-matched normal weight (NW) volunteers in order to assemble age- and obesity-related microbiota profiles.
29922272	3	23	theme	microbiota	341:350	arg1	composition					322:332	the composition	318:332	the composition of gut microbiota in obese adolescents and adults	318:382	We investigated the composition of gut microbiota in obese adolescents and adults compared to age-matched normal weight (NW) volunteers in order to assemble age- and obesity-related microbiota profiles.
29922272	10	24	theme	acid	1370:1373	arg1	glycolysis/gluconeogenesis					1475:1500	glycolysis/gluconeogenesis	1475:1500	glycolysis/gluconeogenesis	1475:1500	Particularly, biosynthesis of primary bile acid and steroid acids, metabolism of fructose, mannose, galactose, butanoate, and pentose phosphate and glycolysis/gluconeogenesis were for the majority associated to obese, while biosynthesis and metabolism of glycan, biosynthesis of secondary bile acid, metabolism of steroid hormone and lipoic acid were associated to NW adolescents.
29922272	10	24	theme	acid	1370:1373	arg1	biosynthesis					1341:1352	biosynthesis	1341:1352	biosynthesis of primary bile acid and steroid acids	1341:1391	Particularly, biosynthesis of primary bile acid and steroid acids, metabolism of fructose, mannose, galactose, butanoate, and pentose phosphate and glycolysis/gluconeogenesis were for the majority associated to obese, while biosynthesis and metabolism of glycan, biosynthesis of secondary bile acid, metabolism of steroid hormone and lipoic acid were associated to NW adolescents.
29922272	10	24	theme	acid	1370:1373	arg1	metabolism					1394:1403	metabolism	1394:1403	metabolism of fructose, mannose, galactose, butanoate, and pentose phosphate	1394:1469	Particularly, biosynthesis of primary bile acid and steroid acids, metabolism of fructose, mannose, galactose, butanoate, and pentose phosphate and glycolysis/gluconeogenesis were for the majority associated to obese, while biosynthesis and metabolism of glycan, biosynthesis of secondary bile acid, metabolism of steroid hormone and lipoic acid were associated to NW adolescents.
29922272	8	25	theme	obese	1107:1111	arg1	adolescents					1113:1123	obese adolescents	1107:1123	obese adolescents	1107:1123	Using statistical analyses, we assigned, as microbial markers, Faecalibacterium prausnitzii and Actinomyces to the microbiota of obese adolescents, and Parabacteroides, Rikenellaceae, Bacteroides caccae, Barnesiellaceae, and Oscillospira to the microbiota of NW adolescents.
29922272	0	26	dep	Patterns	90:97	arg1	Markers					15:21	Gut Microbiota Markers	0:21	Gut Microbiota Markers in Obese Adolescent and Adult Patients	0:60	Gut Microbiota Markers in Obese Adolescent and Adult Patients: Age-Dependent Differential Patterns.
29922272	10	27	theme	primary	1357:1363	arg1	acid					1370:1373	primary bile acid	1357:1373	primary bile acid	1357:1373	Particularly, biosynthesis of primary bile acid and steroid acids, metabolism of fructose, mannose, galactose, butanoate, and pentose phosphate and glycolysis/gluconeogenesis were for the majority associated to obese, while biosynthesis and metabolism of glycan, biosynthesis of secondary bile acid, metabolism of steroid hormone and lipoic acid were associated to NW adolescents.
29922272	3	28	theme	NW	423:424	arg1	volunteers					427:436	age-matched normal weight (NW) volunteers	396:436	age-matched normal weight (NW) volunteers	396:436	We investigated the composition of gut microbiota in obese adolescents and adults compared to age-matched normal weight (NW) volunteers in order to assemble age- and obesity-related microbiota profiles.
29922272	0	29	theme	Gut	0:2	arg1	Markers					15:21	Gut Microbiota Markers	0:21	Gut Microbiota Markers in Obese Adolescent and Adult Patients	0:60	Gut Microbiota Markers in Obese Adolescent and Adult Patients: Age-Dependent Differential Patterns.
29922272	10	30	theme	glycan	1582:1587	arg1	biosynthesis					1590:1601	biosynthesis	1590:1601	biosynthesis of secondary bile acid	1590:1624	Particularly, biosynthesis of primary bile acid and steroid acids, metabolism of fructose, mannose, galactose, butanoate, and pentose phosphate and glycolysis/gluconeogenesis were for the majority associated to obese, while biosynthesis and metabolism of glycan, biosynthesis of secondary bile acid, metabolism of steroid hormone and lipoic acid were associated to NW adolescents.
29922272	10	30	theme	glycan	1582:1587	arg1	metabolism					1627:1636	metabolism	1627:1636	metabolism of steroid hormone	1627:1655	Particularly, biosynthesis of primary bile acid and steroid acids, metabolism of fructose, mannose, galactose, butanoate, and pentose phosphate and glycolysis/gluconeogenesis were for the majority associated to obese, while biosynthesis and metabolism of glycan, biosynthesis of secondary bile acid, metabolism of steroid hormone and lipoic acid were associated to NW adolescents.
29922272	10	30	theme	glycan	1582:1587	arg1	acid					1668:1671	lipoic acid	1661:1671	lipoic acid	1661:1671	Particularly, biosynthesis of primary bile acid and steroid acids, metabolism of fructose, mannose, galactose, butanoate, and pentose phosphate and glycolysis/gluconeogenesis were for the majority associated to obese, while biosynthesis and metabolism of glycan, biosynthesis of secondary bile acid, metabolism of steroid hormone and lipoic acid were associated to NW adolescents.
29922272	10	30	theme	glycan	1582:1587	arg1	metabolism					1568:1577	metabolism	1568:1577	metabolism	1568:1577	Particularly, biosynthesis of primary bile acid and steroid acids, metabolism of fructose, mannose, galactose, butanoate, and pentose phosphate and glycolysis/gluconeogenesis were for the majority associated to obese, while biosynthesis and metabolism of glycan, biosynthesis of secondary bile acid, metabolism of steroid hormone and lipoic acid were associated to NW adolescents.
29922272	10	30	theme	glycan	1582:1587	arg1	biosynthesis					1551:1562	biosynthesis	1551:1562	biosynthesis	1551:1562	Particularly, biosynthesis of primary bile acid and steroid acids, metabolism of fructose, mannose, galactose, butanoate, and pentose phosphate and glycolysis/gluconeogenesis were for the majority associated to obese, while biosynthesis and metabolism of glycan, biosynthesis of secondary bile acid, metabolism of steroid hormone and lipoic acid were associated to NW adolescents.
29922272	12	31	theme	patient-tailored	1940:1955	arg1	treatments					1957:1966	patient-tailored treatments	1940:1966	patient-tailored treatments dependent on age-related microbiota profiles	1940:2011	The assignment of novel obesity bacterial markers may open avenues for the development of patient-tailored treatments dependent on age-related microbiota profiles.
29922272	6	32	from	surveys	806:812	arg1	content					779:785	metagenome functional content	757:785	metagenome functional content from 16S rRNA gene surveys	757:812	The prediction of metagenome functional content from 16S rRNA gene surveys was carried out.
29922272	6	32	from	surveys	806:812	arg1	prediction					743:752	The prediction	739:752	The prediction of metagenome functional content from 16S rRNA gene surveys	739:812	The prediction of metagenome functional content from 16S rRNA gene surveys was carried out.
29922272	3	33	theme	weight	415:420	arg1	volunteers					427:436	age-matched normal weight (NW) volunteers	396:436	age-matched normal weight (NW) volunteers	396:436	We investigated the composition of gut microbiota in obese adolescents and adults compared to age-matched normal weight (NW) volunteers in order to assemble age- and obesity-related microbiota profiles.
29922272	4	34	theme	rRNA-based	559:568	arg1	metagenomics					570:581	rRNA-based metagenomics	559:581	rRNA-based metagenomics	559:581	The composition of gut microbiota was analyzed by 16S rRNA-based metagenomics.
29922272	11	35	theme	ecological	1773:1782	arg1	patterns					1784:1791	ecological patterns	1773:1791	ecological patterns	1773:1791	Our study revealed unique features of gut microbiota in terms of ecological patterns, microbial composition and metabolism in obese patients.
29922272	10	36	theme	steroid	1379:1385	arg1	acids					1387:1391	steroid acids	1379:1391	steroid acids	1379:1391	Particularly, biosynthesis of primary bile acid and steroid acids, metabolism of fructose, mannose, galactose, butanoate, and pentose phosphate and glycolysis/gluconeogenesis were for the majority associated to obese, while biosynthesis and metabolism of glycan, biosynthesis of secondary bile acid, metabolism of steroid hormone and lipoic acid were associated to NW adolescents.
29922272	12	37	theme	dependent	1968:1976	arg1	treatments					1957:1966	patient-tailored treatments	1940:1966	patient-tailored treatments dependent on age-related microbiota profiles	1940:2011	The assignment of novel obesity bacterial markers may open avenues for the development of patient-tailored treatments dependent on age-related microbiota profiles.
29922272	3	38	theme	age-matched	396:406	arg1	volunteers					427:436	age-matched normal weight (NW) volunteers	396:436	age-matched normal weight (NW) volunteers	396:436	We investigated the composition of gut microbiota in obese adolescents and adults compared to age-matched normal weight (NW) volunteers in order to assemble age- and obesity-related microbiota profiles.
29922272	4	39	theme	gut	524:526	arg1	microbiota					528:537	gut microbiota	524:537	gut microbiota	524:537	The composition of gut microbiota was analyzed by 16S rRNA-based metagenomics.
29922272	10	40	theme	secondary	1606:1614	arg1	acid					1621:1624	secondary bile acid	1606:1624	secondary bile acid	1606:1624	Particularly, biosynthesis of primary bile acid and steroid acids, metabolism of fructose, mannose, galactose, butanoate, and pentose phosphate and glycolysis/gluconeogenesis were for the majority associated to obese, while biosynthesis and metabolism of glycan, biosynthesis of secondary bile acid, metabolism of steroid hormone and lipoic acid were associated to NW adolescents.
29922272	6	41	theme	rRNA	796:799	arg1	surveys					806:812	16S rRNA gene surveys	792:812	16S rRNA gene surveys	792:812	The prediction of metagenome functional content from 16S rRNA gene surveys was carried out.
29922272	3	42	theme	microbiota	484:493	arg1	profiles					495:502	age- and obesity-related microbiota profiles	459:502	age- and obesity-related microbiota profiles	459:502	We investigated the composition of gut microbiota in obese adolescents and adults compared to age-matched normal weight (NW) volunteers in order to assemble age- and obesity-related microbiota profiles.
29922272	11	43	theme	composition	1804:1814	arg1	terms					1764:1768	terms	1764:1768	terms of ecological patterns, microbial composition and metabolism in obese patients	1764:1847	Our study revealed unique features of gut microbiota in terms of ecological patterns, microbial composition and metabolism in obese patients.
29922272	1	44	from	levels	108:113	arg1	children					130:137	children	130:137	children	130:137	Obesity levels, especially in children, have dramatically increased over the last few decades.
29922272	6	45	theme	gene	801:804	arg1	surveys					806:812	16S rRNA gene surveys	792:812	16S rRNA gene surveys	792:812	The prediction of metagenome functional content from 16S rRNA gene surveys was carried out.
29922272	12	46	theme	novel	1868:1872	arg1	markers					1892:1898	novel obesity bacterial markers	1868:1898	novel obesity bacterial markers	1868:1898	The assignment of novel obesity bacterial markers may open avenues for the development of patient-tailored treatments dependent on age-related microbiota profiles.
29922272	6	47	theme	functional	768:777	arg1	content					779:785	metagenome functional content	757:785	metagenome functional content from 16S rRNA gene surveys	757:812	The prediction of metagenome functional content from 16S rRNA gene surveys was carried out.
29922272	12	48	theme	bacterial	1882:1890	arg1	markers					1892:1898	novel obesity bacterial markers	1868:1898	novel obesity bacterial markers	1868:1898	The assignment of novel obesity bacterial markers may open avenues for the development of patient-tailored treatments dependent on age-related microbiota profiles.
29922272	10	49	theme	acid	1621:1624	arg1	biosynthesis					1590:1601	biosynthesis	1590:1601	biosynthesis of secondary bile acid	1590:1624	Particularly, biosynthesis of primary bile acid and steroid acids, metabolism of fructose, mannose, galactose, butanoate, and pentose phosphate and glycolysis/gluconeogenesis were for the majority associated to obese, while biosynthesis and metabolism of glycan, biosynthesis of secondary bile acid, metabolism of steroid hormone and lipoic acid were associated to NW adolescents.
29922272	10	49	theme	acid	1621:1624	arg1	metabolism					1627:1636	metabolism	1627:1636	metabolism of steroid hormone	1627:1655	Particularly, biosynthesis of primary bile acid and steroid acids, metabolism of fructose, mannose, galactose, butanoate, and pentose phosphate and glycolysis/gluconeogenesis were for the majority associated to obese, while biosynthesis and metabolism of glycan, biosynthesis of secondary bile acid, metabolism of steroid hormone and lipoic acid were associated to NW adolescents.
29922272	10	49	theme	acid	1621:1624	arg1	acid					1668:1671	lipoic acid	1661:1671	lipoic acid	1661:1671	Particularly, biosynthesis of primary bile acid and steroid acids, metabolism of fructose, mannose, galactose, butanoate, and pentose phosphate and glycolysis/gluconeogenesis were for the majority associated to obese, while biosynthesis and metabolism of glycan, biosynthesis of secondary bile acid, metabolism of steroid hormone and lipoic acid were associated to NW adolescents.
29922272	10	49	theme	acid	1621:1624	arg1	metabolism					1568:1577	metabolism	1568:1577	metabolism	1568:1577	Particularly, biosynthesis of primary bile acid and steroid acids, metabolism of fructose, mannose, galactose, butanoate, and pentose phosphate and glycolysis/gluconeogenesis were for the majority associated to obese, while biosynthesis and metabolism of glycan, biosynthesis of secondary bile acid, metabolism of steroid hormone and lipoic acid were associated to NW adolescents.
29922272	10	49	theme	acid	1621:1624	arg1	biosynthesis					1551:1562	biosynthesis	1551:1562	biosynthesis	1551:1562	Particularly, biosynthesis of primary bile acid and steroid acids, metabolism of fructose, mannose, galactose, butanoate, and pentose phosphate and glycolysis/gluconeogenesis were for the majority associated to obese, while biosynthesis and metabolism of glycan, biosynthesis of secondary bile acid, metabolism of steroid hormone and lipoic acid were associated to NW adolescents.
29922272	11	50	theme	unique	1727:1732	arg1	features					1734:1741	unique features	1727:1741	unique features of gut microbiota	1727:1759	Our study revealed unique features of gut microbiota in terms of ecological patterns, microbial composition and metabolism in obese patients.
29922272	8	51	theme	microbial	1022:1030	arg1	markers					1032:1038	microbial markers	1022:1038	microbial markers	1022:1038	Using statistical analyses, we assigned, as microbial markers, Faecalibacterium prausnitzii and Actinomyces to the microbiota of obese adolescents, and Parabacteroides, Rikenellaceae, Bacteroides caccae, Barnesiellaceae, and Oscillospira to the microbiota of NW adolescents.
29922272	8	51	theme	microbial	1022:1030	arg1	caccae					1174:1179	caccae	1174:1179	caccae	1174:1179	Using statistical analyses, we assigned, as microbial markers, Faecalibacterium prausnitzii and Actinomyces to the microbiota of obese adolescents, and Parabacteroides, Rikenellaceae, Bacteroides caccae, Barnesiellaceae, and Oscillospira to the microbiota of NW adolescents.
29922272	8	51	theme	microbial	1022:1030	arg1	Barnesiellaceae					1182:1196	Barnesiellaceae	1182:1196	Barnesiellaceae	1182:1196	Using statistical analyses, we assigned, as microbial markers, Faecalibacterium prausnitzii and Actinomyces to the microbiota of obese adolescents, and Parabacteroides, Rikenellaceae, Bacteroides caccae, Barnesiellaceae, and Oscillospira to the microbiota of NW adolescents.
29922272	8	51	theme	microbial	1022:1030	arg1	Faecalibacterium					1041:1056	Faecalibacterium	1041:1056	Faecalibacterium	1041:1056	Using statistical analyses, we assigned, as microbial markers, Faecalibacterium prausnitzii and Actinomyces to the microbiota of obese adolescents, and Parabacteroides, Rikenellaceae, Bacteroides caccae, Barnesiellaceae, and Oscillospira to the microbiota of NW adolescents.
29922272	8	51	theme	microbial	1022:1030	arg1	Rikenellaceae					1147:1159	Rikenellaceae	1147:1159	Rikenellaceae	1147:1159	Using statistical analyses, we assigned, as microbial markers, Faecalibacterium prausnitzii and Actinomyces to the microbiota of obese adolescents, and Parabacteroides, Rikenellaceae, Bacteroides caccae, Barnesiellaceae, and Oscillospira to the microbiota of NW adolescents.
29922272	8	51	theme	microbial	1022:1030	arg1	Actinomyces					1074:1084	Actinomyces	1074:1084	Actinomyces	1074:1084	Using statistical analyses, we assigned, as microbial markers, Faecalibacterium prausnitzii and Actinomyces to the microbiota of obese adolescents, and Parabacteroides, Rikenellaceae, Bacteroides caccae, Barnesiellaceae, and Oscillospira to the microbiota of NW adolescents.
29922272	8	51	theme	microbial	1022:1030	arg1	Oscillospira					1203:1214	Oscillospira	1203:1214	Oscillospira	1203:1214	Using statistical analyses, we assigned, as microbial markers, Faecalibacterium prausnitzii and Actinomyces to the microbiota of obese adolescents, and Parabacteroides, Rikenellaceae, Bacteroides caccae, Barnesiellaceae, and Oscillospira to the microbiota of NW adolescents.
29922272	10	52	theme	pentose	1453:1459	arg1	phosphate					1461:1469	pentose phosphate	1453:1469	pentose phosphate	1453:1469	Particularly, biosynthesis of primary bile acid and steroid acids, metabolism of fructose, mannose, galactose, butanoate, and pentose phosphate and glycolysis/gluconeogenesis were for the majority associated to obese, while biosynthesis and metabolism of glycan, biosynthesis of secondary bile acid, metabolism of steroid hormone and lipoic acid were associated to NW adolescents.
29922272	10	53	theme	steroid	1641:1647	arg1	hormone					1649:1655	steroid hormone	1641:1655	steroid hormone	1641:1655	Particularly, biosynthesis of primary bile acid and steroid acids, metabolism of fructose, mannose, galactose, butanoate, and pentose phosphate and glycolysis/gluconeogenesis were for the majority associated to obese, while biosynthesis and metabolism of glycan, biosynthesis of secondary bile acid, metabolism of steroid hormone and lipoic acid were associated to NW adolescents.
29922272	11	54	theme	microbiota	1750:1759	arg1	features					1734:1741	unique features	1727:1741	unique features of gut microbiota	1727:1759	Our study revealed unique features of gut microbiota in terms of ecological patterns, microbial composition and metabolism in obese patients.
29922272	9	55	theme	predicted	1257:1265	arg1	profiles					1277:1284	The predicted metabolic profiles	1253:1284	The predicted metabolic profiles	1253:1284	The predicted metabolic profiles resulted different in adolescent groups.
29922272	7	56	theme	resultant	901:909	arg1	profiles					922:929	resultant microbiota profiles	901:929	resultant microbiota profiles	901:929	Ecological analyses revealed a dissimilarity among the subgroups, and resultant microbiota profiles differed between obese adolescents and adults.
29922272	1	57	theme	last	177:180	arg1	decades					186:192	the last few decades	173:192	the last few decades	173:192	Obesity levels, especially in children, have dramatically increased over the last few decades.
29922272	8	58	theme	adolescents	1240:1250	arg1	microbiota					1223:1232	the microbiota	1219:1232	the microbiota of NW adolescents	1219:1250	Using statistical analyses, we assigned, as microbial markers, Faecalibacterium prausnitzii and Actinomyces to the microbiota of obese adolescents, and Parabacteroides, Rikenellaceae, Bacteroides caccae, Barnesiellaceae, and Oscillospira to the microbiota of NW adolescents.
29922272	6	59	theme	16S	792:794	arg1	surveys					806:812	16S rRNA gene surveys	792:812	16S rRNA gene surveys	792:812	The prediction of metagenome functional content from 16S rRNA gene surveys was carried out.
29922272	9	60	theme	adolescent	1308:1317	arg1	groups					1319:1324	adolescent groups	1308:1324	adolescent groups	1308:1324	The predicted metabolic profiles resulted different in adolescent groups.
29922272	10	61	theme	NW	1692:1693	arg1	adolescents					1695:1705	NW adolescents	1692:1705	NW adolescents	1692:1705	Particularly, biosynthesis of primary bile acid and steroid acids, metabolism of fructose, mannose, galactose, butanoate, and pentose phosphate and glycolysis/gluconeogenesis were for the majority associated to obese, while biosynthesis and metabolism of glycan, biosynthesis of secondary bile acid, metabolism of steroid hormone and lipoic acid were associated to NW adolescents.
29922272	11	62	from	metabolism	1820:1829	arg1	patients					1840:1847	obese patients	1834:1847	obese patients	1834:1847	Our study revealed unique features of gut microbiota in terms of ecological patterns, microbial composition and metabolism in obese patients.
29922272	3	63	from	composition	322:332	arg1	adults					377:382	adults	377:382	adults	377:382	We investigated the composition of gut microbiota in obese adolescents and adults compared to age-matched normal weight (NW) volunteers in order to assemble age- and obesity-related microbiota profiles.
29922272	3	63	from	composition	322:332	arg1	adolescents					361:371	obese adolescents	355:371	obese adolescents	355:371	We investigated the composition of gut microbiota in obese adolescents and adults compared to age-matched normal weight (NW) volunteers in order to assemble age- and obesity-related microbiota profiles.
29922272	2	64	theme	microbiota	256:265	arg1	involvement					237:247	the involvement	233:247	the involvement of gut microbiota in the pathophysiology of obesity	233:299	Recently, several studies highlighted the involvement of gut microbiota in the pathophysiology of obesity.
29922272	11	65	theme	obese	1834:1838	arg1	patients					1840:1847	obese patients	1834:1847	obese patients	1834:1847	Our study revealed unique features of gut microbiota in terms of ecological patterns, microbial composition and metabolism in obese patients.
29922272	11	66	from	terms	1764:1768	arg1	patients					1840:1847	obese patients	1834:1847	obese patients	1834:1847	Our study revealed unique features of gut microbiota in terms of ecological patterns, microbial composition and metabolism in obese patients.
29922272	3	67	theme	gut	337:339	arg1	microbiota					341:350	gut microbiota	337:350	gut microbiota	337:350	We investigated the composition of gut microbiota in obese adolescents and adults compared to age-matched normal weight (NW) volunteers in order to assemble age- and obesity-related microbiota profiles.
29922272	11	68	theme	metabolism	1820:1829	arg1	terms					1764:1768	terms	1764:1768	terms of ecological patterns, microbial composition and metabolism in obese patients	1764:1847	Our study revealed unique features of gut microbiota in terms of ecological patterns, microbial composition and metabolism in obese patients.
29922272	0	69	theme	Differential	77:88	arg1	Patterns					90:97	Age-Dependent Differential Patterns	63:97	Age-Dependent Differential Patterns	63:97	Gut Microbiota Markers in Obese Adolescent and Adult Patients: Age-Dependent Differential Patterns.
29922272	11	70	from	composition	1804:1814	arg1	patients					1840:1847	obese patients	1834:1847	obese patients	1834:1847	Our study revealed unique features of gut microbiota in terms of ecological patterns, microbial composition and metabolism in obese patients.
29922272	5	71	theme	communities	624:634	arg1	representations					595:609	Ecological representations	584:609	Ecological representations of microbial communities	584:634	Ecological representations of microbial communities were computed, and univariate, multivariate, and correlation analyses performed on bacterial profiles.
29922272	6	72	theme	content	779:785	arg1	prediction					743:752	The prediction	739:752	The prediction of metagenome functional content from 16S rRNA gene surveys	739:812	The prediction of metagenome functional content from 16S rRNA gene surveys was carried out.
29922272	8	73	theme	adolescents	1113:1123	arg1	microbiota					1093:1102	the microbiota	1089:1102	the microbiota of obese adolescents	1089:1123	Using statistical analyses, we assigned, as microbial markers, Faecalibacterium prausnitzii and Actinomyces to the microbiota of obese adolescents, and Parabacteroides, Rikenellaceae, Bacteroides caccae, Barnesiellaceae, and Oscillospira to the microbiota of NW adolescents.
29922272	10	74	theme	bile	1365:1368	arg1	acid					1370:1373	primary bile acid	1357:1373	primary bile acid	1357:1373	Particularly, biosynthesis of primary bile acid and steroid acids, metabolism of fructose, mannose, galactose, butanoate, and pentose phosphate and glycolysis/gluconeogenesis were for the majority associated to obese, while biosynthesis and metabolism of glycan, biosynthesis of secondary bile acid, metabolism of steroid hormone and lipoic acid were associated to NW adolescents.
29922272	0	75	theme	Microbiota	4:13	arg1	Markers					15:21	Gut Microbiota Markers	0:21	Gut Microbiota Markers in Obese Adolescent and Adult Patients	0:60	Gut Microbiota Markers in Obese Adolescent and Adult Patients: Age-Dependent Differential Patterns.
29922272	5	76	theme	Ecological	584:593	arg1	representations					595:609	Ecological representations	584:609	Ecological representations of microbial communities	584:634	Ecological representations of microbial communities were computed, and univariate, multivariate, and correlation analyses performed on bacterial profiles.
29922272	12	77	theme	microbiota	1993:2002	arg1	profiles					2004:2011	age-related microbiota profiles	1981:2011	age-related microbiota profiles	1981:2011	The assignment of novel obesity bacterial markers may open avenues for the development of patient-tailored treatments dependent on age-related microbiota profiles.
29922272	12	78	theme	treatments	1957:1966	arg1	development					1925:1935	the development	1921:1935	the development of patient-tailored treatments dependent on age-related microbiota profiles	1921:2011	The assignment of novel obesity bacterial markers may open avenues for the development of patient-tailored treatments dependent on age-related microbiota profiles.
29922272	1	79	theme	Obesity	100:106	arg1	levels					108:113	Obesity levels	100:113	Obesity levels	100:113	Obesity levels, especially in children, have dramatically increased over the last few decades.
29922272	2	80	from	involvement	237:247	arg1	pathophysiology					274:288	the pathophysiology	270:288	the pathophysiology of obesity	270:299	Recently, several studies highlighted the involvement of gut microbiota in the pathophysiology of obesity.
29922272	10	81	theme	acids	1387:1391	arg1	glycolysis/gluconeogenesis					1475:1500	glycolysis/gluconeogenesis	1475:1500	glycolysis/gluconeogenesis	1475:1500	Particularly, biosynthesis of primary bile acid and steroid acids, metabolism of fructose, mannose, galactose, butanoate, and pentose phosphate and glycolysis/gluconeogenesis were for the majority associated to obese, while biosynthesis and metabolism of glycan, biosynthesis of secondary bile acid, metabolism of steroid hormone and lipoic acid were associated to NW adolescents.
29922272	10	81	theme	acids	1387:1391	arg1	biosynthesis					1341:1352	biosynthesis	1341:1352	biosynthesis of primary bile acid and steroid acids	1341:1391	Particularly, biosynthesis of primary bile acid and steroid acids, metabolism of fructose, mannose, galactose, butanoate, and pentose phosphate and glycolysis/gluconeogenesis were for the majority associated to obese, while biosynthesis and metabolism of glycan, biosynthesis of secondary bile acid, metabolism of steroid hormone and lipoic acid were associated to NW adolescents.
29922272	10	81	theme	acids	1387:1391	arg1	metabolism					1394:1403	metabolism	1394:1403	metabolism of fructose, mannose, galactose, butanoate, and pentose phosphate	1394:1469	Particularly, biosynthesis of primary bile acid and steroid acids, metabolism of fructose, mannose, galactose, butanoate, and pentose phosphate and glycolysis/gluconeogenesis were for the majority associated to obese, while biosynthesis and metabolism of glycan, biosynthesis of secondary bile acid, metabolism of steroid hormone and lipoic acid were associated to NW adolescents.
29922272	3	82	theme	age-	459:462	arg1	profiles					495:502	age- and obesity-related microbiota profiles	459:502	age- and obesity-related microbiota profiles	459:502	We investigated the composition of gut microbiota in obese adolescents and adults compared to age-matched normal weight (NW) volunteers in order to assemble age- and obesity-related microbiota profiles.
29922272	7	83	theme	Ecological	831:840	arg1	analyses					842:849	Ecological analyses	831:849	Ecological analyses	831:849	Ecological analyses revealed a dissimilarity among the subgroups, and resultant microbiota profiles differed between obese adolescents and adults.
29922272	4	84	theme	microbiota	528:537	arg1	composition					509:519	The composition	505:519	The composition of gut microbiota	505:537	The composition of gut microbiota was analyzed by 16S rRNA-based metagenomics.
29922272	10	85	theme	mannose	1418:1424	arg1	glycolysis/gluconeogenesis					1475:1500	glycolysis/gluconeogenesis	1475:1500	glycolysis/gluconeogenesis	1475:1500	Particularly, biosynthesis of primary bile acid and steroid acids, metabolism of fructose, mannose, galactose, butanoate, and pentose phosphate and glycolysis/gluconeogenesis were for the majority associated to obese, while biosynthesis and metabolism of glycan, biosynthesis of secondary bile acid, metabolism of steroid hormone and lipoic acid were associated to NW adolescents.
29922272	10	85	theme	mannose	1418:1424	arg1	biosynthesis					1341:1352	biosynthesis	1341:1352	biosynthesis of primary bile acid and steroid acids	1341:1391	Particularly, biosynthesis of primary bile acid and steroid acids, metabolism of fructose, mannose, galactose, butanoate, and pentose phosphate and glycolysis/gluconeogenesis were for the majority associated to obese, while biosynthesis and metabolism of glycan, biosynthesis of secondary bile acid, metabolism of steroid hormone and lipoic acid were associated to NW adolescents.
29922272	10	85	theme	mannose	1418:1424	arg1	metabolism					1394:1403	metabolism	1394:1403	metabolism of fructose, mannose, galactose, butanoate, and pentose phosphate	1394:1469	Particularly, biosynthesis of primary bile acid and steroid acids, metabolism of fructose, mannose, galactose, butanoate, and pentose phosphate and glycolysis/gluconeogenesis were for the majority associated to obese, while biosynthesis and metabolism of glycan, biosynthesis of secondary bile acid, metabolism of steroid hormone and lipoic acid were associated to NW adolescents.
29922272	0	86	dep	Adolescent	32:41	arg1	Patients					53:60	Patients	53:60	Patients	53:60	Gut Microbiota Markers in Obese Adolescent and Adult Patients: Age-Dependent Differential Patterns.
29922272	12	87	theme	markers	1892:1898	arg1	assignment					1854:1863	The assignment	1850:1863	The assignment of novel obesity bacterial markers	1850:1898	The assignment of novel obesity bacterial markers may open avenues for the development of patient-tailored treatments dependent on age-related microbiota profiles.
29922272	3	88	theme	normal	408:413	arg1	volunteers					427:436	age-matched normal weight (NW) volunteers	396:436	age-matched normal weight (NW) volunteers	396:436	We investigated the composition of gut microbiota in obese adolescents and adults compared to age-matched normal weight (NW) volunteers in order to assemble age- and obesity-related microbiota profiles.
29922272	11	89	theme	microbial	1794:1802	arg1	composition					1804:1814	microbial composition	1794:1814	microbial composition	1794:1814	Our study revealed unique features of gut microbiota in terms of ecological patterns, microbial composition and metabolism in obese patients.
29922272	12	90	theme	age-related	1981:1991	arg1	profiles					2004:2011	age-related microbiota profiles	1981:2011	age-related microbiota profiles	1981:2011	The assignment of novel obesity bacterial markers may open avenues for the development of patient-tailored treatments dependent on age-related microbiota profiles.
29922272	10	91	theme	fructose	1408:1415	arg1	glycolysis/gluconeogenesis					1475:1500	glycolysis/gluconeogenesis	1475:1500	glycolysis/gluconeogenesis	1475:1500	Particularly, biosynthesis of primary bile acid and steroid acids, metabolism of fructose, mannose, galactose, butanoate, and pentose phosphate and glycolysis/gluconeogenesis were for the majority associated to obese, while biosynthesis and metabolism of glycan, biosynthesis of secondary bile acid, metabolism of steroid hormone and lipoic acid were associated to NW adolescents.
29922272	10	91	theme	fructose	1408:1415	arg1	biosynthesis					1341:1352	biosynthesis	1341:1352	biosynthesis of primary bile acid and steroid acids	1341:1391	Particularly, biosynthesis of primary bile acid and steroid acids, metabolism of fructose, mannose, galactose, butanoate, and pentose phosphate and glycolysis/gluconeogenesis were for the majority associated to obese, while biosynthesis and metabolism of glycan, biosynthesis of secondary bile acid, metabolism of steroid hormone and lipoic acid were associated to NW adolescents.
29922272	10	91	theme	fructose	1408:1415	arg1	metabolism					1394:1403	metabolism	1394:1403	metabolism of fructose, mannose, galactose, butanoate, and pentose phosphate	1394:1469	Particularly, biosynthesis of primary bile acid and steroid acids, metabolism of fructose, mannose, galactose, butanoate, and pentose phosphate and glycolysis/gluconeogenesis were for the majority associated to obese, while biosynthesis and metabolism of glycan, biosynthesis of secondary bile acid, metabolism of steroid hormone and lipoic acid were associated to NW adolescents.
29922272	8	92	theme	statistical	984:994	arg1	analyses					996:1003	statistical analyses	984:1003	statistical analyses	984:1003	Using statistical analyses, we assigned, as microbial markers, Faecalibacterium prausnitzii and Actinomyces to the microbiota of obese adolescents, and Parabacteroides, Rikenellaceae, Bacteroides caccae, Barnesiellaceae, and Oscillospira to the microbiota of NW adolescents.
29922272	6	93	theme	metagenome	757:766	arg1	content					779:785	metagenome functional content	757:785	metagenome functional content from 16S rRNA gene surveys	757:812	The prediction of metagenome functional content from 16S rRNA gene surveys was carried out.
29922272	11	94	theme	patterns	1784:1791	arg1	terms					1764:1768	terms	1764:1768	terms of ecological patterns, microbial composition and metabolism in obese patients	1764:1847	Our study revealed unique features of gut microbiota in terms of ecological patterns, microbial composition and metabolism in obese patients.
29922272	10	95	theme	butanoate	1438:1446	arg1	glycolysis/gluconeogenesis					1475:1500	glycolysis/gluconeogenesis	1475:1500	glycolysis/gluconeogenesis	1475:1500	Particularly, biosynthesis of primary bile acid and steroid acids, metabolism of fructose, mannose, galactose, butanoate, and pentose phosphate and glycolysis/gluconeogenesis were for the majority associated to obese, while biosynthesis and metabolism of glycan, biosynthesis of secondary bile acid, metabolism of steroid hormone and lipoic acid were associated to NW adolescents.
29922272	10	95	theme	butanoate	1438:1446	arg1	biosynthesis					1341:1352	biosynthesis	1341:1352	biosynthesis of primary bile acid and steroid acids	1341:1391	Particularly, biosynthesis of primary bile acid and steroid acids, metabolism of fructose, mannose, galactose, butanoate, and pentose phosphate and glycolysis/gluconeogenesis were for the majority associated to obese, while biosynthesis and metabolism of glycan, biosynthesis of secondary bile acid, metabolism of steroid hormone and lipoic acid were associated to NW adolescents.
29922272	10	95	theme	butanoate	1438:1446	arg1	metabolism					1394:1403	metabolism	1394:1403	metabolism of fructose, mannose, galactose, butanoate, and pentose phosphate	1394:1469	Particularly, biosynthesis of primary bile acid and steroid acids, metabolism of fructose, mannose, galactose, butanoate, and pentose phosphate and glycolysis/gluconeogenesis were for the majority associated to obese, while biosynthesis and metabolism of glycan, biosynthesis of secondary bile acid, metabolism of steroid hormone and lipoic acid were associated to NW adolescents.
29922272	3	96	theme	obesity-related	468:482	arg1	microbiota					484:493	obesity-related microbiota	468:493	obesity-related microbiota	468:493	We investigated the composition of gut microbiota in obese adolescents and adults compared to age-matched normal weight (NW) volunteers in order to assemble age- and obesity-related microbiota profiles.
29922272	7	97	theme	obese	948:952	arg1	adolescents					954:964	obese adolescents	948:964	obese adolescents	948:964	Ecological analyses revealed a dissimilarity among the subgroups, and resultant microbiota profiles differed between obese adolescents and adults.
29922272	10	98	theme	galactose	1427:1435	arg1	glycolysis/gluconeogenesis					1475:1500	glycolysis/gluconeogenesis	1475:1500	glycolysis/gluconeogenesis	1475:1500	Particularly, biosynthesis of primary bile acid and steroid acids, metabolism of fructose, mannose, galactose, butanoate, and pentose phosphate and glycolysis/gluconeogenesis were for the majority associated to obese, while biosynthesis and metabolism of glycan, biosynthesis of secondary bile acid, metabolism of steroid hormone and lipoic acid were associated to NW adolescents.
29922272	10	98	theme	galactose	1427:1435	arg1	biosynthesis					1341:1352	biosynthesis	1341:1352	biosynthesis of primary bile acid and steroid acids	1341:1391	Particularly, biosynthesis of primary bile acid and steroid acids, metabolism of fructose, mannose, galactose, butanoate, and pentose phosphate and glycolysis/gluconeogenesis were for the majority associated to obese, while biosynthesis and metabolism of glycan, biosynthesis of secondary bile acid, metabolism of steroid hormone and lipoic acid were associated to NW adolescents.
29922272	10	98	theme	galactose	1427:1435	arg1	metabolism					1394:1403	metabolism	1394:1403	metabolism of fructose, mannose, galactose, butanoate, and pentose phosphate	1394:1469	Particularly, biosynthesis of primary bile acid and steroid acids, metabolism of fructose, mannose, galactose, butanoate, and pentose phosphate and glycolysis/gluconeogenesis were for the majority associated to obese, while biosynthesis and metabolism of glycan, biosynthesis of secondary bile acid, metabolism of steroid hormone and lipoic acid were associated to NW adolescents.
31384746	3	0	theme	human	671:675	arg1	cells					695:699	primary human airway epithelial cells	663:699	primary human airway epithelial cells of nasal origin (hAECN)	663:723	We evaluated effectiveness and quality of newly developed and previous A(H1N1)pdm09 LAIV reassortants through assessment of their thermal and pH stability, receptor binding specificity and replication fitness in primary human airway epithelial cells of nasal origin (hAECN).
31384746	5	1	theme	SA	1341:1342	arg1	preference					1361:1370	an α2,3 SA receptor binding preference	1333:1370	an α2,3 SA receptor binding preference	1333:1370	Reassortants A/South Africa/3626/2013-CDC-LV14A (SA-LV14A) and NY-LV16A showed preferential binding to α2,6 sialic acid (SA) receptors and replicated at higher titers and more extensively in hAECN compared to A17/Cal09 and A17/Bol13, which had an α2,3 SA receptor binding preference.
31384746	1	2	theme	recent	133:138	arg1	reduction					140:148	The recent reduction	129:148	The recent reduction of live attenuated influenza vaccine (LAIV) effectiveness in multivalent formulations	129:234	The recent reduction of live attenuated influenza vaccine (LAIV) effectiveness in multivalent formulations was particularly associated with the A(H1N1)pdm09 component.
31384746	5	3	theme	α2,6	1192:1195	arg1	receptors					1214:1222	α2,6 sialic acid (SA) receptors	1192:1222	α2,6 sialic acid (SA) receptors	1192:1222	Reassortants A/South Africa/3626/2013-CDC-LV14A (SA-LV14A) and NY-LV16A showed preferential binding to α2,6 sialic acid (SA) receptors and replicated at higher titers and more extensively in hAECN compared to A17/Cal09 and A17/Bol13, which had an α2,3 SA receptor binding preference.
31384746	4	4	theme	pH	935:936	arg1	stability					938:946	higher thermal and pH stability	916:946	stability	938:946	Our analysis showed that LAIV expressed hemagglutinin (HA) and neuraminidase (NA) from an A/Michigan/45/2015-like strain A/New York/61/2015 (A/New York/61/2015-CDC-LV16A, NY-LV16A), exhibit higher thermal and pH stability compared to the previous vaccine candidates expressing HA and NA from A/California/07/2009 and A/Bolivia/559/2013 (A17/Cal09 and A17/Bol13).
31384746	5	5	dep	showed	1161:1166	arg1	replicated					1228:1237	replicated	1228:1237	replicated at higher titers	1228:1254	Reassortants A/South Africa/3626/2013-CDC-LV14A (SA-LV14A) and NY-LV16A showed preferential binding to α2,6 sialic acid (SA) receptors and replicated at higher titers and more extensively in hAECN compared to A17/Cal09 and A17/Bol13, which had an α2,3 SA receptor binding preference.
31384746	5	5	dep	showed	1161:1166	arg1	extensively					1265:1275	extensively	1265:1275	extensively	1265:1275	Reassortants A/South Africa/3626/2013-CDC-LV14A (SA-LV14A) and NY-LV16A showed preferential binding to α2,6 sialic acid (SA) receptors and replicated at higher titers and more extensively in hAECN compared to A17/Cal09 and A17/Bol13, which had an α2,3 SA receptor binding preference.
31384746	4	6	dep	A/California/07/2009	1018:1037	arg1	A17/Bol13					1077:1085	A17/Bol13	1077:1085	A17/Bol13	1077:1085	Our analysis showed that LAIV expressed hemagglutinin (HA) and neuraminidase (NA) from an A/Michigan/45/2015-like strain A/New York/61/2015 (A/New York/61/2015-CDC-LV16A, NY-LV16A), exhibit higher thermal and pH stability compared to the previous vaccine candidates expressing HA and NA from A/California/07/2009 and A/Bolivia/559/2013 (A17/Cal09 and A17/Bol13).
31384746	4	6	dep	A/California/07/2009	1018:1037	arg1	A17/Cal09					1063:1071	A17/Cal09	1063:1071	A17/Cal09	1063:1071	Our analysis showed that LAIV expressed hemagglutinin (HA) and neuraminidase (NA) from an A/Michigan/45/2015-like strain A/New York/61/2015 (A/New York/61/2015-CDC-LV16A, NY-LV16A), exhibit higher thermal and pH stability compared to the previous vaccine candidates expressing HA and NA from A/California/07/2009 and A/Bolivia/559/2013 (A17/Cal09 and A17/Bol13).
31384746	5	7	contain	had	1329:1331	arg2	preference					1361:1370	an α2,3 SA receptor binding preference	1333:1370	an α2,3 SA receptor binding preference	1333:1370	Reassortants A/South Africa/3626/2013-CDC-LV14A (SA-LV14A) and NY-LV16A showed preferential binding to α2,6 sialic acid (SA) receptors and replicated at higher titers and more extensively in hAECN compared to A17/Cal09 and A17/Bol13, which had an α2,3 SA receptor binding preference.
31384746	5	7	contain	had	1329:1331	arg1	A17/Cal09					1298:1306	A17/Cal09	1298:1306	A17/Cal09	1298:1306	Reassortants A/South Africa/3626/2013-CDC-LV14A (SA-LV14A) and NY-LV16A showed preferential binding to α2,6 sialic acid (SA) receptors and replicated at higher titers and more extensively in hAECN compared to A17/Cal09 and A17/Bol13, which had an α2,3 SA receptor binding preference.
31384746	5	7	contain	had	1329:1331	arg1	A17/Bol13					1312:1320	A17/Bol13	1312:1320	A17/Bol13	1312:1320	Reassortants A/South Africa/3626/2013-CDC-LV14A (SA-LV14A) and NY-LV16A showed preferential binding to α2,6 sialic acid (SA) receptors and replicated at higher titers and more extensively in hAECN compared to A17/Cal09 and A17/Bol13, which had an α2,3 SA receptor binding preference.
31384746	5	8	theme	higher	1242:1247	arg1	titers					1249:1254	higher titers	1242:1254	higher titers	1242:1254	Reassortants A/South Africa/3626/2013-CDC-LV14A (SA-LV14A) and NY-LV16A showed preferential binding to α2,6 sialic acid (SA) receptors and replicated at higher titers and more extensively in hAECN compared to A17/Cal09 and A17/Bol13, which had an α2,3 SA receptor binding preference.
31384746	5	9	theme	sialic	1197:1202	arg1	acid					1204:1207	sialic acid	1197:1207	α2,6 sialic acid (SA) receptors	1192:1222	Reassortants A/South Africa/3626/2013-CDC-LV14A (SA-LV14A) and NY-LV16A showed preferential binding to α2,6 sialic acid (SA) receptors and replicated at higher titers and more extensively in hAECN compared to A17/Cal09 and A17/Bol13, which had an α2,3 SA receptor binding preference.
31384746	5	9	theme	sialic	1197:1202	arg1	SA					1210:1211	SA	1210:1211	SA	1210:1211	Reassortants A/South Africa/3626/2013-CDC-LV14A (SA-LV14A) and NY-LV16A showed preferential binding to α2,6 sialic acid (SA) receptors and replicated at higher titers and more extensively in hAECN compared to A17/Cal09 and A17/Bol13, which had an α2,3 SA receptor binding preference.
31384746	4	10	theme	A/New	867:871	arg1	York/61/2015-CDC-LV16A					873:894	A/New York/61/2015-CDC-LV16A	867:894	A/New York/61/2015-CDC-LV16A	867:894	Our analysis showed that LAIV expressed hemagglutinin (HA) and neuraminidase (NA) from an A/Michigan/45/2015-like strain A/New York/61/2015 (A/New York/61/2015-CDC-LV16A, NY-LV16A), exhibit higher thermal and pH stability compared to the previous vaccine candidates expressing HA and NA from A/California/07/2009 and A/Bolivia/559/2013 (A17/Cal09 and A17/Bol13).
31384746	4	10	theme	A/New	867:871	arg1	NY-LV16A					897:904	NY-LV16A	897:904	NY-LV16A	897:904	Our analysis showed that LAIV expressed hemagglutinin (HA) and neuraminidase (NA) from an A/Michigan/45/2015-like strain A/New York/61/2015 (A/New York/61/2015-CDC-LV16A, NY-LV16A), exhibit higher thermal and pH stability compared to the previous vaccine candidates expressing HA and NA from A/California/07/2009 and A/Bolivia/559/2013 (A17/Cal09 and A17/Bol13).
31384746	0	11	from	Evaluation	0:9	arg1	cells					122:126	primary human nasal epithelial cells	91:126	primary human nasal epithelial cells	91:126	Evaluation of A(H1N1)pdm09 LAIV vaccine candidates stability and replication efficiency in primary human nasal epithelial cells.
31384746	4	12	theme	A/Michigan/45/2015-like	816:838	arg1	York/61/2015					853:864	an A/Michigan/45/2015-like strain A/New York/61/2015	813:864	an A/Michigan/45/2015-like strain A/New York/61/2015 (A/New York/61/2015-CDC-LV16A, NY-LV16A)	813:905	Our analysis showed that LAIV expressed hemagglutinin (HA) and neuraminidase (NA) from an A/Michigan/45/2015-like strain A/New York/61/2015 (A/New York/61/2015-CDC-LV16A, NY-LV16A), exhibit higher thermal and pH stability compared to the previous vaccine candidates expressing HA and NA from A/California/07/2009 and A/Bolivia/559/2013 (A17/Cal09 and A17/Bol13).
31384746	1	13	from	reduction	140:148	arg1	formulations					223:234	multivalent formulations	211:234	multivalent formulations	211:234	The recent reduction of live attenuated influenza vaccine (LAIV) effectiveness in multivalent formulations was particularly associated with the A(H1N1)pdm09 component.
31384746	3	14	theme	previous	513:520	arg1	H1N1					524:527	H1N1	524:527	H1N1	524:527	We evaluated effectiveness and quality of newly developed and previous A(H1N1)pdm09 LAIV reassortants through assessment of their thermal and pH stability, receptor binding specificity and replication fitness in primary human airway epithelial cells of nasal origin (hAECN).
31384746	3	14	theme	previous	513:520	arg1	A					522:522	newly developed and previous A	493:522	newly developed and previous A(H1N1)pdm09 LAIV reassortants	493:551	We evaluated effectiveness and quality of newly developed and previous A(H1N1)pdm09 LAIV reassortants through assessment of their thermal and pH stability, receptor binding specificity and replication fitness in primary human airway epithelial cells of nasal origin (hAECN).
31384746	3	15	from	specificity	624:634	arg1	cells					695:699	primary human airway epithelial cells	663:699	primary human airway epithelial cells of nasal origin (hAECN)	663:723	We evaluated effectiveness and quality of newly developed and previous A(H1N1)pdm09 LAIV reassortants through assessment of their thermal and pH stability, receptor binding specificity and replication fitness in primary human airway epithelial cells of nasal origin (hAECN).
31384746	5	16	theme	acid	1204:1207	arg1	receptors					1214:1222	α2,6 sialic acid (SA) receptors	1192:1222	α2,6 sialic acid (SA) receptors	1192:1222	Reassortants A/South Africa/3626/2013-CDC-LV14A (SA-LV14A) and NY-LV16A showed preferential binding to α2,6 sialic acid (SA) receptors and replicated at higher titers and more extensively in hAECN compared to A17/Cal09 and A17/Bol13, which had an α2,3 SA receptor binding preference.
31384746	0	17	theme	replication	65:75	arg1	efficiency					77:86	replication efficiency	65:86	replication efficiency	65:86	Evaluation of A(H1N1)pdm09 LAIV vaccine candidates stability and replication efficiency in primary human nasal epithelial cells.
31384746	0	17	theme	replication	65:75	arg1	candidates					40:49	A(H1N1)pdm09 LAIV vaccine candidates	14:49	A(H1N1)pdm09 LAIV vaccine candidates stability and replication efficiency	14:86	Evaluation of A(H1N1)pdm09 LAIV vaccine candidates stability and replication efficiency in primary human nasal epithelial cells.
31384746	3	18	theme	A	522:522	arg1	reassortants					540:551	newly developed and previous A(H1N1)pdm09 LAIV reassortants	493:551	newly developed and previous A(H1N1)pdm09 LAIV reassortants	493:551	We evaluated effectiveness and quality of newly developed and previous A(H1N1)pdm09 LAIV reassortants through assessment of their thermal and pH stability, receptor binding specificity and replication fitness in primary human airway epithelial cells of nasal origin (hAECN).
31384746	2	19	theme	vaccine	317:323	arg1	composition					325:335	vaccine composition committee	317:345	the WHO vaccine composition committee	309:345	In the 2017 the WHO vaccine composition committee changed its recommendations for the A(H1N1)pdm09 component to include an A/Michigan/45/2015-like virus.
31384746	0	20	theme	primary	91:97	arg1	cells					122:126	primary human nasal epithelial cells	91:126	primary human nasal epithelial cells	91:126	Evaluation of A(H1N1)pdm09 LAIV vaccine candidates stability and replication efficiency in primary human nasal epithelial cells.
31384746	3	21	theme	developed	499:507	arg1	H1N1					524:527	H1N1	524:527	H1N1	524:527	We evaluated effectiveness and quality of newly developed and previous A(H1N1)pdm09 LAIV reassortants through assessment of their thermal and pH stability, receptor binding specificity and replication fitness in primary human airway epithelial cells of nasal origin (hAECN).
31384746	3	21	theme	developed	499:507	arg1	A					522:522	newly developed and previous A	493:522	newly developed and previous A(H1N1)pdm09 LAIV reassortants	493:551	We evaluated effectiveness and quality of newly developed and previous A(H1N1)pdm09 LAIV reassortants through assessment of their thermal and pH stability, receptor binding specificity and replication fitness in primary human airway epithelial cells of nasal origin (hAECN).
31384746	4	22	dep	expressed	756:764	arg1	exhibit					908:914	exhibit	908:914	exhibit higher thermal and pH stability compared to the previous vaccine candidates expressing HA and NA from A/California/07/2009 and A/Bolivia/559/2013 (A17/Cal09 and A17/Bol13)	908:1086	Our analysis showed that LAIV expressed hemagglutinin (HA) and neuraminidase (NA) from an A/Michigan/45/2015-like strain A/New York/61/2015 (A/New York/61/2015-CDC-LV16A, NY-LV16A), exhibit higher thermal and pH stability compared to the previous vaccine candidates expressing HA and NA from A/California/07/2009 and A/Bolivia/559/2013 (A17/Cal09 and A17/Bol13).
31384746	3	23	theme	airway	677:682	arg1	cells					695:699	primary human airway epithelial cells	663:699	primary human airway epithelial cells of nasal origin (hAECN)	663:723	We evaluated effectiveness and quality of newly developed and previous A(H1N1)pdm09 LAIV reassortants through assessment of their thermal and pH stability, receptor binding specificity and replication fitness in primary human airway epithelial cells of nasal origin (hAECN).
31384746	5	24	theme	A/South	1102:1108	arg1	SA-LV14A					1138:1145	SA-LV14A	1138:1145	SA-LV14A	1138:1145	Reassortants A/South Africa/3626/2013-CDC-LV14A (SA-LV14A) and NY-LV16A showed preferential binding to α2,6 sialic acid (SA) receptors and replicated at higher titers and more extensively in hAECN compared to A17/Cal09 and A17/Bol13, which had an α2,3 SA receptor binding preference.
31384746	5	24	theme	A/South	1102:1108	arg1	Africa/3626/2013-CDC-LV14A					1110:1135	A/South Africa/3626/2013-CDC-LV14A	1102:1135	A/South Africa/3626/2013-CDC-LV14A (SA-LV14A)	1102:1146	Reassortants A/South Africa/3626/2013-CDC-LV14A (SA-LV14A) and NY-LV16A showed preferential binding to α2,6 sialic acid (SA) receptors and replicated at higher titers and more extensively in hAECN compared to A17/Cal09 and A17/Bol13, which had an α2,3 SA receptor binding preference.
31384746	5	24	theme	A/South	1102:1108	arg1	Reassortants					1089:1100	Reassortants A/South Africa/3626/2013-CDC-LV14A (SA-LV14A) and NY-LV16A	1089:1159	Reassortants A/South Africa/3626/2013-CDC-LV14A (SA-LV14A) and NY-LV16A	1089:1159	Reassortants A/South Africa/3626/2013-CDC-LV14A (SA-LV14A) and NY-LV16A showed preferential binding to α2,6 sialic acid (SA) receptors and replicated at higher titers and more extensively in hAECN compared to A17/Cal09 and A17/Bol13, which had an α2,3 SA receptor binding preference.
31384746	2	25	dep	composition	325:335	arg1	WHO					313:315	WHO	313:315	WHO	313:315	In the 2017 the WHO vaccine composition committee changed its recommendations for the A(H1N1)pdm09 component to include an A/Michigan/45/2015-like virus.
31384746	1	26	theme	live	153:156	arg1	LAIV					188:191	LAIV	188:191	LAIV	188:191	The recent reduction of live attenuated influenza vaccine (LAIV) effectiveness in multivalent formulations was particularly associated with the A(H1N1)pdm09 component.
31384746	1	26	theme	live	153:156	arg1	vaccine					179:185	live attenuated influenza vaccine	153:185	live attenuated influenza vaccine (LAIV) effectiveness	153:206	The recent reduction of live attenuated influenza vaccine (LAIV) effectiveness in multivalent formulations was particularly associated with the A(H1N1)pdm09 component.
31384746	6	27	theme	York/61/2015-CDC-LV16A	1419:1440	arg1	selection					1400:1408	selection	1400:1408	selection of A/New York/61/2015-CDC-LV16A for LAIV formulation and the introduction of new assays for LAIV characterization	1400:1522	Our data analysis supports selection of A/New York/61/2015-CDC-LV16A for LAIV formulation and the introduction of new assays for LAIV characterization.
31384746	3	28	theme	epithelial	684:693	arg1	cells					695:699	primary human airway epithelial cells	663:699	primary human airway epithelial cells of nasal origin (hAECN)	663:723	We evaluated effectiveness and quality of newly developed and previous A(H1N1)pdm09 LAIV reassortants through assessment of their thermal and pH stability, receptor binding specificity and replication fitness in primary human airway epithelial cells of nasal origin (hAECN).
31384746	5	29	theme	preferential	1168:1179	arg1	binding					1181:1187	preferential binding	1168:1187	preferential binding to α2,6 sialic acid (SA) receptors	1168:1222	Reassortants A/South Africa/3626/2013-CDC-LV14A (SA-LV14A) and NY-LV16A showed preferential binding to α2,6 sialic acid (SA) receptors and replicated at higher titers and more extensively in hAECN compared to A17/Cal09 and A17/Bol13, which had an α2,3 SA receptor binding preference.
31384746	6	30	theme	LAIV	1446:1449	arg1	formulation					1451:1461	LAIV formulation	1446:1461	LAIV formulation	1446:1461	Our data analysis supports selection of A/New York/61/2015-CDC-LV16A for LAIV formulation and the introduction of new assays for LAIV characterization.
31384746	1	31	theme	attenuated	158:167	arg1	LAIV					188:191	LAIV	188:191	LAIV	188:191	The recent reduction of live attenuated influenza vaccine (LAIV) effectiveness in multivalent formulations was particularly associated with the A(H1N1)pdm09 component.
31384746	1	31	theme	attenuated	158:167	arg1	vaccine					179:185	live attenuated influenza vaccine	153:185	live attenuated influenza vaccine (LAIV) effectiveness	153:206	The recent reduction of live attenuated influenza vaccine (LAIV) effectiveness in multivalent formulations was particularly associated with the A(H1N1)pdm09 component.
31384746	4	32	theme	vaccine	973:979	arg1	candidates					981:990	the previous vaccine candidates	960:990	the previous vaccine candidates expressing HA and NA from A/California/07/2009 and A/Bolivia/559/2013 (A17/Cal09 and A17/Bol13)	960:1086	Our analysis showed that LAIV expressed hemagglutinin (HA) and neuraminidase (NA) from an A/Michigan/45/2015-like strain A/New York/61/2015 (A/New York/61/2015-CDC-LV16A, NY-LV16A), exhibit higher thermal and pH stability compared to the previous vaccine candidates expressing HA and NA from A/California/07/2009 and A/Bolivia/559/2013 (A17/Cal09 and A17/Bol13).
31384746	4	33	theme	previous	964:971	arg1	candidates					981:990	the previous vaccine candidates	960:990	the previous vaccine candidates expressing HA and NA from A/California/07/2009 and A/Bolivia/559/2013 (A17/Cal09 and A17/Bol13)	960:1086	Our analysis showed that LAIV expressed hemagglutinin (HA) and neuraminidase (NA) from an A/Michigan/45/2015-like strain A/New York/61/2015 (A/New York/61/2015-CDC-LV16A, NY-LV16A), exhibit higher thermal and pH stability compared to the previous vaccine candidates expressing HA and NA from A/California/07/2009 and A/Bolivia/559/2013 (A17/Cal09 and A17/Bol13).
31384746	3	34	theme	pdm09	529:533	arg1	reassortants					540:551	newly developed and previous A(H1N1)pdm09 LAIV reassortants	493:551	newly developed and previous A(H1N1)pdm09 LAIV reassortants	493:551	We evaluated effectiveness and quality of newly developed and previous A(H1N1)pdm09 LAIV reassortants through assessment of their thermal and pH stability, receptor binding specificity and replication fitness in primary human airway epithelial cells of nasal origin (hAECN).
31384746	1	35	theme	influenza	169:177	arg1	LAIV					188:191	LAIV	188:191	LAIV	188:191	The recent reduction of live attenuated influenza vaccine (LAIV) effectiveness in multivalent formulations was particularly associated with the A(H1N1)pdm09 component.
31384746	1	35	theme	influenza	169:177	arg1	vaccine					179:185	live attenuated influenza vaccine	153:185	live attenuated influenza vaccine (LAIV) effectiveness	153:206	The recent reduction of live attenuated influenza vaccine (LAIV) effectiveness in multivalent formulations was particularly associated with the A(H1N1)pdm09 component.
31384746	2	36	theme	pdm09	390:394	arg1	component					396:404	the A(H1N1)pdm09 component	379:404	the A(H1N1)pdm09 component	379:404	In the 2017 the WHO vaccine composition committee changed its recommendations for the A(H1N1)pdm09 component to include an A/Michigan/45/2015-like virus.
31384746	1	37	theme	A	273:273	arg1	component					286:294	the A(H1N1)pdm09 component	269:294	the A(H1N1)pdm09 component	269:294	The recent reduction of live attenuated influenza vaccine (LAIV) effectiveness in multivalent formulations was particularly associated with the A(H1N1)pdm09 component.
31384746	0	38	theme	nasal	105:109	arg1	cells					122:126	primary human nasal epithelial cells	91:126	primary human nasal epithelial cells	91:126	Evaluation of A(H1N1)pdm09 LAIV vaccine candidates stability and replication efficiency in primary human nasal epithelial cells.
31384746	0	39	dep	candidates	40:49	arg1	stability					51:59	stability	51:59	stability	51:59	Evaluation of A(H1N1)pdm09 LAIV vaccine candidates stability and replication efficiency in primary human nasal epithelial cells.
31384746	0	39	dep	candidates	40:49	arg1	efficiency					77:86	replication efficiency	65:86	replication efficiency	65:86	Evaluation of A(H1N1)pdm09 LAIV vaccine candidates stability and replication efficiency in primary human nasal epithelial cells.
31384746	0	39	dep	candidates	40:49	arg1	candidates					40:49	A(H1N1)pdm09 LAIV vaccine candidates	14:49	A(H1N1)pdm09 LAIV vaccine candidates stability and replication efficiency	14:86	Evaluation of A(H1N1)pdm09 LAIV vaccine candidates stability and replication efficiency in primary human nasal epithelial cells.
31384746	6	40	theme	LAIV	1502:1505	arg1	characterization					1507:1522	LAIV characterization	1502:1522	LAIV characterization	1502:1522	Our data analysis supports selection of A/New York/61/2015-CDC-LV16A for LAIV formulation and the introduction of new assays for LAIV characterization.
31384746	1	41	theme	vaccine	179:185	arg1	effectiveness					194:206	live attenuated influenza vaccine (LAIV) effectiveness	153:206	live attenuated influenza vaccine (LAIV) effectiveness	153:206	The recent reduction of live attenuated influenza vaccine (LAIV) effectiveness in multivalent formulations was particularly associated with the A(H1N1)pdm09 component.
31384746	0	42	theme	A	14:14	arg1	stability					51:59	stability	51:59	stability	51:59	Evaluation of A(H1N1)pdm09 LAIV vaccine candidates stability and replication efficiency in primary human nasal epithelial cells.
31384746	0	42	theme	A	14:14	arg1	efficiency					77:86	replication efficiency	65:86	replication efficiency	65:86	Evaluation of A(H1N1)pdm09 LAIV vaccine candidates stability and replication efficiency in primary human nasal epithelial cells.
31384746	0	42	theme	A	14:14	arg1	candidates					40:49	A(H1N1)pdm09 LAIV vaccine candidates	14:49	A(H1N1)pdm09 LAIV vaccine candidates stability and replication efficiency	14:86	Evaluation of A(H1N1)pdm09 LAIV vaccine candidates stability and replication efficiency in primary human nasal epithelial cells.
31384746	3	43	theme	nasal	704:708	arg1	hAECN					718:722	hAECN	718:722	hAECN	718:722	We evaluated effectiveness and quality of newly developed and previous A(H1N1)pdm09 LAIV reassortants through assessment of their thermal and pH stability, receptor binding specificity and replication fitness in primary human airway epithelial cells of nasal origin (hAECN).
31384746	3	43	theme	nasal	704:708	arg1	origin					710:715	nasal origin	704:715	nasal origin (hAECN)	704:723	We evaluated effectiveness and quality of newly developed and previous A(H1N1)pdm09 LAIV reassortants through assessment of their thermal and pH stability, receptor binding specificity and replication fitness in primary human airway epithelial cells of nasal origin (hAECN).
31384746	0	44	theme	human	99:103	arg1	cells					122:126	primary human nasal epithelial cells	91:126	primary human nasal epithelial cells	91:126	Evaluation of A(H1N1)pdm09 LAIV vaccine candidates stability and replication efficiency in primary human nasal epithelial cells.
31384746	6	45	theme	assays	1491:1496	arg1	formulation					1451:1461	LAIV formulation	1446:1461	LAIV formulation	1446:1461	Our data analysis supports selection of A/New York/61/2015-CDC-LV16A for LAIV formulation and the introduction of new assays for LAIV characterization.
31384746	6	45	theme	assays	1491:1496	arg1	introduction					1471:1482	the introduction	1467:1482	the introduction of new assays for LAIV characterization	1467:1522	Our data analysis supports selection of A/New York/61/2015-CDC-LV16A for LAIV formulation and the introduction of new assays for LAIV characterization.
31384746	5	46	dep	Reassortants	1089:1100	arg1	SA-LV14A					1138:1145	SA-LV14A	1138:1145	SA-LV14A	1138:1145	Reassortants A/South Africa/3626/2013-CDC-LV14A (SA-LV14A) and NY-LV16A showed preferential binding to α2,6 sialic acid (SA) receptors and replicated at higher titers and more extensively in hAECN compared to A17/Cal09 and A17/Bol13, which had an α2,3 SA receptor binding preference.
31384746	5	46	dep	Reassortants	1089:1100	arg1	NY-LV16A					1152:1159	NY-LV16A	1152:1159	NY-LV16A	1152:1159	Reassortants A/South Africa/3626/2013-CDC-LV14A (SA-LV14A) and NY-LV16A showed preferential binding to α2,6 sialic acid (SA) receptors and replicated at higher titers and more extensively in hAECN compared to A17/Cal09 and A17/Bol13, which had an α2,3 SA receptor binding preference.
31384746	5	46	dep	Reassortants	1089:1100	arg1	Africa/3626/2013-CDC-LV14A					1110:1135	A/South Africa/3626/2013-CDC-LV14A	1102:1135	A/South Africa/3626/2013-CDC-LV14A (SA-LV14A)	1102:1146	Reassortants A/South Africa/3626/2013-CDC-LV14A (SA-LV14A) and NY-LV16A showed preferential binding to α2,6 sialic acid (SA) receptors and replicated at higher titers and more extensively in hAECN compared to A17/Cal09 and A17/Bol13, which had an α2,3 SA receptor binding preference.
31384746	5	46	dep	Reassortants	1089:1100	arg1	Reassortants					1089:1100	Reassortants A/South Africa/3626/2013-CDC-LV14A (SA-LV14A) and NY-LV16A	1089:1159	Reassortants A/South Africa/3626/2013-CDC-LV14A (SA-LV14A) and NY-LV16A	1089:1159	Reassortants A/South Africa/3626/2013-CDC-LV14A (SA-LV14A) and NY-LV16A showed preferential binding to α2,6 sialic acid (SA) receptors and replicated at higher titers and more extensively in hAECN compared to A17/Cal09 and A17/Bol13, which had an α2,3 SA receptor binding preference.
31384746	3	47	from	fitness	652:658	arg1	cells					695:699	primary human airway epithelial cells	663:699	primary human airway epithelial cells of nasal origin (hAECN)	663:723	We evaluated effectiveness and quality of newly developed and previous A(H1N1)pdm09 LAIV reassortants through assessment of their thermal and pH stability, receptor binding specificity and replication fitness in primary human airway epithelial cells of nasal origin (hAECN).
31384746	3	48	theme	origin	710:715	arg1	cells					695:699	primary human airway epithelial cells	663:699	primary human airway epithelial cells of nasal origin (hAECN)	663:723	We evaluated effectiveness and quality of newly developed and previous A(H1N1)pdm09 LAIV reassortants through assessment of their thermal and pH stability, receptor binding specificity and replication fitness in primary human airway epithelial cells of nasal origin (hAECN).
31384746	3	49	theme	receptor	607:614	arg1	specificity					624:634	receptor binding specificity	607:634	receptor binding specificity	607:634	We evaluated effectiveness and quality of newly developed and previous A(H1N1)pdm09 LAIV reassortants through assessment of their thermal and pH stability, receptor binding specificity and replication fitness in primary human airway epithelial cells of nasal origin (hAECN).
31384746	3	50	from	assessment	561:570	arg1	cells					695:699	primary human airway epithelial cells	663:699	primary human airway epithelial cells of nasal origin (hAECN)	663:723	We evaluated effectiveness and quality of newly developed and previous A(H1N1)pdm09 LAIV reassortants through assessment of their thermal and pH stability, receptor binding specificity and replication fitness in primary human airway epithelial cells of nasal origin (hAECN).
31384746	2	51	theme	A	383:383	arg1	component					396:404	the A(H1N1)pdm09 component	379:404	the A(H1N1)pdm09 component	379:404	In the 2017 the WHO vaccine composition committee changed its recommendations for the A(H1N1)pdm09 component to include an A/Michigan/45/2015-like virus.
31384746	2	52	theme	A/Michigan/45/2015-like	420:442	arg1	virus					444:448	an A/Michigan/45/2015-like virus	417:448	an A/Michigan/45/2015-like virus	417:448	In the 2017 the WHO vaccine composition committee changed its recommendations for the A(H1N1)pdm09 component to include an A/Michigan/45/2015-like virus.
31384746	0	53	theme	epithelial	111:120	arg1	cells					122:126	primary human nasal epithelial cells	91:126	primary human nasal epithelial cells	91:126	Evaluation of A(H1N1)pdm09 LAIV vaccine candidates stability and replication efficiency in primary human nasal epithelial cells.
31384746	3	54	theme	pH	593:594	arg1	stability					596:604	their thermal and pH stability	575:604	stability	596:604	We evaluated effectiveness and quality of newly developed and previous A(H1N1)pdm09 LAIV reassortants through assessment of their thermal and pH stability, receptor binding specificity and replication fitness in primary human airway epithelial cells of nasal origin (hAECN).
31384746	5	55	theme	receptor	1344:1351	arg1	preference					1361:1370	an α2,3 SA receptor binding preference	1333:1370	an α2,3 SA receptor binding preference	1333:1370	Reassortants A/South Africa/3626/2013-CDC-LV14A (SA-LV14A) and NY-LV16A showed preferential binding to α2,6 sialic acid (SA) receptors and replicated at higher titers and more extensively in hAECN compared to A17/Cal09 and A17/Bol13, which had an α2,3 SA receptor binding preference.
31384746	0	56	theme	LAIV	27:30	arg1	stability					51:59	stability	51:59	stability	51:59	Evaluation of A(H1N1)pdm09 LAIV vaccine candidates stability and replication efficiency in primary human nasal epithelial cells.
31384746	0	56	theme	LAIV	27:30	arg1	efficiency					77:86	replication efficiency	65:86	replication efficiency	65:86	Evaluation of A(H1N1)pdm09 LAIV vaccine candidates stability and replication efficiency in primary human nasal epithelial cells.
31384746	0	56	theme	LAIV	27:30	arg1	candidates					40:49	A(H1N1)pdm09 LAIV vaccine candidates	14:49	A(H1N1)pdm09 LAIV vaccine candidates stability and replication efficiency	14:86	Evaluation of A(H1N1)pdm09 LAIV vaccine candidates stability and replication efficiency in primary human nasal epithelial cells.
31384746	1	57	theme	pdm09	280:284	arg1	component					286:294	the A(H1N1)pdm09 component	269:294	the A(H1N1)pdm09 component	269:294	The recent reduction of live attenuated influenza vaccine (LAIV) effectiveness in multivalent formulations was particularly associated with the A(H1N1)pdm09 component.
31384746	4	58	theme	A/New	847:851	arg1	York/61/2015					853:864	an A/Michigan/45/2015-like strain A/New York/61/2015	813:864	an A/Michigan/45/2015-like strain A/New York/61/2015 (A/New York/61/2015-CDC-LV16A, NY-LV16A)	813:905	Our analysis showed that LAIV expressed hemagglutinin (HA) and neuraminidase (NA) from an A/Michigan/45/2015-like strain A/New York/61/2015 (A/New York/61/2015-CDC-LV16A, NY-LV16A), exhibit higher thermal and pH stability compared to the previous vaccine candidates expressing HA and NA from A/California/07/2009 and A/Bolivia/559/2013 (A17/Cal09 and A17/Bol13).
31384746	3	59	theme	stability	596:604	arg1	specificity					624:634	receptor binding specificity	607:634	receptor binding specificity	607:634	We evaluated effectiveness and quality of newly developed and previous A(H1N1)pdm09 LAIV reassortants through assessment of their thermal and pH stability, receptor binding specificity and replication fitness in primary human airway epithelial cells of nasal origin (hAECN).
31384746	3	59	theme	stability	596:604	arg1	fitness					652:658	replication fitness	640:658	replication fitness	640:658	We evaluated effectiveness and quality of newly developed and previous A(H1N1)pdm09 LAIV reassortants through assessment of their thermal and pH stability, receptor binding specificity and replication fitness in primary human airway epithelial cells of nasal origin (hAECN).
31384746	3	59	theme	stability	596:604	arg1	assessment					561:570	assessment	561:570	assessment of their thermal and pH stability	561:604	We evaluated effectiveness and quality of newly developed and previous A(H1N1)pdm09 LAIV reassortants through assessment of their thermal and pH stability, receptor binding specificity and replication fitness in primary human airway epithelial cells of nasal origin (hAECN).
31384746	3	60	theme	LAIV	535:538	arg1	reassortants					540:551	newly developed and previous A(H1N1)pdm09 LAIV reassortants	493:551	newly developed and previous A(H1N1)pdm09 LAIV reassortants	493:551	We evaluated effectiveness and quality of newly developed and previous A(H1N1)pdm09 LAIV reassortants through assessment of their thermal and pH stability, receptor binding specificity and replication fitness in primary human airway epithelial cells of nasal origin (hAECN).
31384746	5	61	theme	binding	1353:1359	arg1	preference					1361:1370	an α2,3 SA receptor binding preference	1333:1370	an α2,3 SA receptor binding preference	1333:1370	Reassortants A/South Africa/3626/2013-CDC-LV14A (SA-LV14A) and NY-LV16A showed preferential binding to α2,6 sialic acid (SA) receptors and replicated at higher titers and more extensively in hAECN compared to A17/Cal09 and A17/Bol13, which had an α2,3 SA receptor binding preference.
31384746	6	62	theme	A/New	1413:1417	arg1	York/61/2015-CDC-LV16A					1419:1440	A/New York/61/2015-CDC-LV16A	1413:1440	A/New York/61/2015-CDC-LV16A	1413:1440	Our data analysis supports selection of A/New York/61/2015-CDC-LV16A for LAIV formulation and the introduction of new assays for LAIV characterization.
31384746	6	63	theme	data	1377:1380	arg1	analysis					1382:1389	Our data analysis	1373:1389	Our data analysis	1373:1389	Our data analysis supports selection of A/New York/61/2015-CDC-LV16A for LAIV formulation and the introduction of new assays for LAIV characterization.
31384746	4	64	theme	strain	840:845	arg1	York/61/2015					853:864	an A/Michigan/45/2015-like strain A/New York/61/2015	813:864	an A/Michigan/45/2015-like strain A/New York/61/2015 (A/New York/61/2015-CDC-LV16A, NY-LV16A)	813:905	Our analysis showed that LAIV expressed hemagglutinin (HA) and neuraminidase (NA) from an A/Michigan/45/2015-like strain A/New York/61/2015 (A/New York/61/2015-CDC-LV16A, NY-LV16A), exhibit higher thermal and pH stability compared to the previous vaccine candidates expressing HA and NA from A/California/07/2009 and A/Bolivia/559/2013 (A17/Cal09 and A17/Bol13).
31384746	3	65	theme	reassortants	540:551	arg1	quality					482:488	quality	482:488	quality	482:488	We evaluated effectiveness and quality of newly developed and previous A(H1N1)pdm09 LAIV reassortants through assessment of their thermal and pH stability, receptor binding specificity and replication fitness in primary human airway epithelial cells of nasal origin (hAECN).
31384746	3	65	theme	reassortants	540:551	arg1	effectiveness					464:476	effectiveness	464:476	effectiveness	464:476	We evaluated effectiveness and quality of newly developed and previous A(H1N1)pdm09 LAIV reassortants through assessment of their thermal and pH stability, receptor binding specificity and replication fitness in primary human airway epithelial cells of nasal origin (hAECN).
31384746	6	66	theme	new	1487:1489	arg1	assays					1491:1496	new assays	1487:1496	new assays for LAIV characterization	1487:1522	Our data analysis supports selection of A/New York/61/2015-CDC-LV16A for LAIV formulation and the introduction of new assays for LAIV characterization.
31384746	3	67	theme	replication	640:650	arg1	fitness					652:658	replication fitness	640:658	replication fitness	640:658	We evaluated effectiveness and quality of newly developed and previous A(H1N1)pdm09 LAIV reassortants through assessment of their thermal and pH stability, receptor binding specificity and replication fitness in primary human airway epithelial cells of nasal origin (hAECN).
31384746	3	68	theme	thermal	581:587	arg1	specificity					624:634	receptor binding specificity	607:634	receptor binding specificity	607:634	We evaluated effectiveness and quality of newly developed and previous A(H1N1)pdm09 LAIV reassortants through assessment of their thermal and pH stability, receptor binding specificity and replication fitness in primary human airway epithelial cells of nasal origin (hAECN).
31384746	3	68	theme	thermal	581:587	arg1	fitness					652:658	replication fitness	640:658	replication fitness	640:658	We evaluated effectiveness and quality of newly developed and previous A(H1N1)pdm09 LAIV reassortants through assessment of their thermal and pH stability, receptor binding specificity and replication fitness in primary human airway epithelial cells of nasal origin (hAECN).
31384746	3	68	theme	thermal	581:587	arg1	assessment					561:570	assessment	561:570	assessment of their thermal and pH stability	561:604	We evaluated effectiveness and quality of newly developed and previous A(H1N1)pdm09 LAIV reassortants through assessment of their thermal and pH stability, receptor binding specificity and replication fitness in primary human airway epithelial cells of nasal origin (hAECN).
31384746	1	69	theme	effectiveness	194:206	arg1	reduction					140:148	The recent reduction	129:148	The recent reduction of live attenuated influenza vaccine (LAIV) effectiveness in multivalent formulations	129:234	The recent reduction of live attenuated influenza vaccine (LAIV) effectiveness in multivalent formulations was particularly associated with the A(H1N1)pdm09 component.
31384746	4	70	dep	thermal	923:929	arg1	higher					916:921	higher	916:921	higher	916:921	Our analysis showed that LAIV expressed hemagglutinin (HA) and neuraminidase (NA) from an A/Michigan/45/2015-like strain A/New York/61/2015 (A/New York/61/2015-CDC-LV16A, NY-LV16A), exhibit higher thermal and pH stability compared to the previous vaccine candidates expressing HA and NA from A/California/07/2009 and A/Bolivia/559/2013 (A17/Cal09 and A17/Bol13).
31384746	3	71	theme	binding	616:622	arg1	specificity					624:634	receptor binding specificity	607:634	receptor binding specificity	607:634	We evaluated effectiveness and quality of newly developed and previous A(H1N1)pdm09 LAIV reassortants through assessment of their thermal and pH stability, receptor binding specificity and replication fitness in primary human airway epithelial cells of nasal origin (hAECN).
31384746	0	72	theme	candidates	40:49	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of A(H1N1)pdm09 LAIV vaccine candidates stability and replication efficiency in primary human nasal epithelial cells.	0:127	Evaluation of A(H1N1)pdm09 LAIV vaccine candidates stability and replication efficiency in primary human nasal epithelial cells.
31384746	1	73	theme	multivalent	211:221	arg1	formulations					223:234	multivalent formulations	211:234	multivalent formulations	211:234	The recent reduction of live attenuated influenza vaccine (LAIV) effectiveness in multivalent formulations was particularly associated with the A(H1N1)pdm09 component.
31384746	0	74	theme	vaccine	32:38	arg1	stability					51:59	stability	51:59	stability	51:59	Evaluation of A(H1N1)pdm09 LAIV vaccine candidates stability and replication efficiency in primary human nasal epithelial cells.
31384746	0	74	theme	vaccine	32:38	arg1	efficiency					77:86	replication efficiency	65:86	replication efficiency	65:86	Evaluation of A(H1N1)pdm09 LAIV vaccine candidates stability and replication efficiency in primary human nasal epithelial cells.
31384746	0	74	theme	vaccine	32:38	arg1	candidates					40:49	A(H1N1)pdm09 LAIV vaccine candidates	14:49	A(H1N1)pdm09 LAIV vaccine candidates stability and replication efficiency	14:86	Evaluation of A(H1N1)pdm09 LAIV vaccine candidates stability and replication efficiency in primary human nasal epithelial cells.
31384746	3	75	theme	primary	663:669	arg1	cells					695:699	primary human airway epithelial cells	663:699	primary human airway epithelial cells of nasal origin (hAECN)	663:723	We evaluated effectiveness and quality of newly developed and previous A(H1N1)pdm09 LAIV reassortants through assessment of their thermal and pH stability, receptor binding specificity and replication fitness in primary human airway epithelial cells of nasal origin (hAECN).
31384746	4	76	dep	York/61/2015	853:864	arg1	York/61/2015-CDC-LV16A					873:894	A/New York/61/2015-CDC-LV16A	867:894	A/New York/61/2015-CDC-LV16A	867:894	Our analysis showed that LAIV expressed hemagglutinin (HA) and neuraminidase (NA) from an A/Michigan/45/2015-like strain A/New York/61/2015 (A/New York/61/2015-CDC-LV16A, NY-LV16A), exhibit higher thermal and pH stability compared to the previous vaccine candidates expressing HA and NA from A/California/07/2009 and A/Bolivia/559/2013 (A17/Cal09 and A17/Bol13).
31384746	4	76	dep	York/61/2015	853:864	arg1	NY-LV16A					897:904	NY-LV16A	897:904	NY-LV16A	897:904	Our analysis showed that LAIV expressed hemagglutinin (HA) and neuraminidase (NA) from an A/Michigan/45/2015-like strain A/New York/61/2015 (A/New York/61/2015-CDC-LV16A, NY-LV16A), exhibit higher thermal and pH stability compared to the previous vaccine candidates expressing HA and NA from A/California/07/2009 and A/Bolivia/559/2013 (A17/Cal09 and A17/Bol13).
31384746	5	77	theme	α2,3	1336:1339	arg1	preference					1361:1370	an α2,3 SA receptor binding preference	1333:1370	an α2,3 SA receptor binding preference	1333:1370	Reassortants A/South Africa/3626/2013-CDC-LV14A (SA-LV14A) and NY-LV16A showed preferential binding to α2,6 sialic acid (SA) receptors and replicated at higher titers and more extensively in hAECN compared to A17/Cal09 and A17/Bol13, which had an α2,3 SA receptor binding preference.
31508799	5	0	theme	signal	1053:1058	arg1	sites					1112:1116	glycosylphosphatidylinositol modification sites	1070:1116	glycosylphosphatidylinositol modification sites	1070:1116	Main package features include prediction of proline hydroxylation sites, amino acid motif and bias analyses, efficient communication with web servers for prediction of N-terminal signal peptides, glycosylphosphatidylinositol modification sites and disordered regions and the ability to annotate sequences through hmmscan and subsequent GO enrichment, based on predicted Pfam domains.
31508799	5	0	theme	signal	1053:1058	arg1	regions					1133:1139	disordered regions	1122:1139	disordered regions	1122:1139	Main package features include prediction of proline hydroxylation sites, amino acid motif and bias analyses, efficient communication with web servers for prediction of N-terminal signal peptides, glycosylphosphatidylinositol modification sites and disordered regions and the ability to annotate sequences through hmmscan and subsequent GO enrichment, based on predicted Pfam domains.
31508799	5	0	theme	signal	1053:1058	arg1	peptides					1060:1067	N-terminal signal peptides	1042:1067	N-terminal signal peptides	1042:1067	Main package features include prediction of proline hydroxylation sites, amino acid motif and bias analyses, efficient communication with web servers for prediction of N-terminal signal peptides, glycosylphosphatidylinositol modification sites and disordered regions and the ability to annotate sequences through hmmscan and subsequent GO enrichment, based on predicted Pfam domains.
31508799	5	1	dep	enrichment	1213:1222	arg1	GO					1210:1211	GO	1210:1211	GO	1210:1211	Main package features include prediction of proline hydroxylation sites, amino acid motif and bias analyses, efficient communication with web servers for prediction of N-terminal signal peptides, glycosylphosphatidylinositol modification sites and disordered regions and the ability to annotate sequences through hmmscan and subsequent GO enrichment, based on predicted Pfam domains.
31508799	0	2	with	mining	19:24	arg1	implementation					74:87	implementation	74:87	implementation in R. Hydroxyproline-rich glycoproteins (HRGPs)	74:135	ragp: Pipeline for mining of plant hydroxyproline-rich glycoproteins with implementation in R. Hydroxyproline-rich glycoproteins (HRGPs) are one of the most complex families of macromolecules found in plants, due to the diversity of glycans decorating the protein backbone, as well as the heterogeneity of the protein backbones.
31508799	5	3	theme	package	879:885	arg1	features					887:894	Main package features	874:894	Main package features	874:894	Main package features include prediction of proline hydroxylation sites, amino acid motif and bias analyses, efficient communication with web servers for prediction of N-terminal signal peptides, glycosylphosphatidylinositol modification sites and disordered regions and the ability to annotate sequences through hmmscan and subsequent GO enrichment, based on predicted Pfam domains.
31508799	6	4	theme	data	1328:1331	arg1	analyses					1333:1340	high-throughput sequence data analyses	1303:1340	high-throughput sequence data analyses	1303:1340	As such, ragp extends R's rich ecosystem for high-throughput sequence data analyses.
31508799	0	5	theme	families	165:172	arg1	families					165:172	the most complex families	148:172	the most complex families of macromolecules found in plants, due to the diversity of glycans decorating the protein backbone, as well as the heterogeneity of the protein backbones	148:326	ragp: Pipeline for mining of plant hydroxyproline-rich glycoproteins with implementation in R. Hydroxyproline-rich glycoproteins (HRGPs) are one of the most complex families of macromolecules found in plants, due to the diversity of glycans decorating the protein backbone, as well as the heterogeneity of the protein backbones.
31508799	0	5	theme	families	165:172	arg1	one					141:143	one	141:143	one	141:143	ragp: Pipeline for mining of plant hydroxyproline-rich glycoproteins with implementation in R. Hydroxyproline-rich glycoproteins (HRGPs) are one of the most complex families of macromolecules found in plants, due to the diversity of glycans decorating the protein backbone, as well as the heterogeneity of the protein backbones.
31508799	0	5	theme	families	165:172	arg1	ragp					0:3	ragp	0:3	ragp: Pipeline for mining of plant hydroxyproline-rich glycoproteins with implementation in R. Hydroxyproline-rich glycoproteins (HRGPs) are one of the most complex families of macromolecules found in plants, due to the diversity of glycans decorating the protein backbone, as well as the heterogeneity of the protein backbones.	0:327	ragp: Pipeline for mining of plant hydroxyproline-rich glycoproteins with implementation in R. Hydroxyproline-rich glycoproteins (HRGPs) are one of the most complex families of macromolecules found in plants, due to the diversity of glycans decorating the protein backbone, as well as the heterogeneity of the protein backbones.
31508799	2	6	theme	amino	576:580	arg1	composition					587:597	biased amino acid composition	569:597	biased amino acid composition	569:597	Current approaches, based on identifying sequences with characteristic motifs and biased amino acid composition, are limited to prototypical sequences.
31508799	5	7	theme	bias	968:971	arg1	analyses					973:980	bias analyses	968:980	bias analyses	968:980	Main package features include prediction of proline hydroxylation sites, amino acid motif and bias analyses, efficient communication with web servers for prediction of N-terminal signal peptides, glycosylphosphatidylinositol modification sites and disordered regions and the ability to annotate sequences through hmmscan and subsequent GO enrichment, based on predicted Pfam domains.
31508799	0	8	theme	Hydroxyproline-rich	95:113	arg1	HRGPs					130:134	HRGPs	130:134	HRGPs	130:134	ragp: Pipeline for mining of plant hydroxyproline-rich glycoproteins with implementation in R. Hydroxyproline-rich glycoproteins (HRGPs) are one of the most complex families of macromolecules found in plants, due to the diversity of glycans decorating the protein backbone, as well as the heterogeneity of the protein backbones.
31508799	0	8	theme	Hydroxyproline-rich	95:113	arg1	glycoproteins					115:127	R. Hydroxyproline-rich glycoproteins	92:127	R. Hydroxyproline-rich glycoproteins (HRGPs)	92:135	ragp: Pipeline for mining of plant hydroxyproline-rich glycoproteins with implementation in R. Hydroxyproline-rich glycoproteins (HRGPs) are one of the most complex families of macromolecules found in plants, due to the diversity of glycans decorating the protein backbone, as well as the heterogeneity of the protein backbones.
31508799	2	9	theme	acid	582:585	arg1	composition					587:597	biased amino acid composition	569:597	biased amino acid composition	569:597	Current approaches, based on identifying sequences with characteristic motifs and biased amino acid composition, are limited to prototypical sequences.
31508799	0	10	gly	glycoproteins	55:67	arg1	glycoproteins					55:67	plant hydroxyproline-rich glycoproteins	29:67	plant hydroxyproline-rich glycoproteins	29:67	ragp: Pipeline for mining of plant hydroxyproline-rich glycoproteins with implementation in R. Hydroxyproline-rich glycoproteins (HRGPs) are one of the most complex families of macromolecules found in plants, due to the diversity of glycans decorating the protein backbone, as well as the heterogeneity of the protein backbones.
31508799	1	11	dep	hinders	435:441	arg1	attempts					443:450	attempts	443:450	hinders attempts for homology based identification	435:484	While this diversity is responsible for a wide array of physiological functions associated with HRGPs, it hinders attempts for homology based identification.
31508799	0	12	theme	macromolecules	177:190	arg1	families					165:172	the most complex families	148:172	the most complex families of macromolecules found in plants, due to the diversity of glycans decorating the protein backbone, as well as the heterogeneity of the protein backbones	148:326	ragp: Pipeline for mining of plant hydroxyproline-rich glycoproteins with implementation in R. Hydroxyproline-rich glycoproteins (HRGPs) are one of the most complex families of macromolecules found in plants, due to the diversity of glycans decorating the protein backbone, as well as the heterogeneity of the protein backbones.
31508799	6	13	theme	R's	1280:1282	arg1	ecosystem					1289:1297	R's rich ecosystem	1280:1297	R's rich ecosystem for high-throughput sequence data analyses	1280:1340	As such, ragp extends R's rich ecosystem for high-throughput sequence data analyses.
31508799	5	14	theme	motif	958:962	arg1	prediction					904:913	prediction	904:913	prediction of proline hydroxylation sites, amino acid motif and bias analyses	904:980	Main package features include prediction of proline hydroxylation sites, amino acid motif and bias analyses, efficient communication with web servers for prediction of N-terminal signal peptides, glycosylphosphatidylinositol modification sites and disordered regions and the ability to annotate sequences through hmmscan and subsequent GO enrichment, based on predicted Pfam domains.
31508799	5	14	theme	motif	958:962	arg1	communication					993:1005	efficient communication	983:1005	efficient communication with web servers for prediction of N-terminal signal peptides, glycosylphosphatidylinositol modification sites and disordered regions	983:1139	Main package features include prediction of proline hydroxylation sites, amino acid motif and bias analyses, efficient communication with web servers for prediction of N-terminal signal peptides, glycosylphosphatidylinositol modification sites and disordered regions and the ability to annotate sequences through hmmscan and subsequent GO enrichment, based on predicted Pfam domains.
31508799	5	14	theme	motif	958:962	arg1	ability					1149:1155	the ability	1145:1155	the ability to annotate sequences through hmmscan and subsequent GO enrichment, based on predicted Pfam domains	1145:1255	Main package features include prediction of proline hydroxylation sites, amino acid motif and bias analyses, efficient communication with web servers for prediction of N-terminal signal peptides, glycosylphosphatidylinositol modification sites and disordered regions and the ability to annotate sequences through hmmscan and subsequent GO enrichment, based on predicted Pfam domains.
31508799	0	15	theme	protein	310:316	arg1	backbones					318:326	the protein backbones	306:326	the protein backbones	306:326	ragp: Pipeline for mining of plant hydroxyproline-rich glycoproteins with implementation in R. Hydroxyproline-rich glycoproteins (HRGPs) are one of the most complex families of macromolecules found in plants, due to the diversity of glycans decorating the protein backbone, as well as the heterogeneity of the protein backbones.
31508799	5	16	theme	predicted	1234:1242	arg1	domains					1249:1255	predicted Pfam domains	1234:1255	predicted Pfam domains	1234:1255	Main package features include prediction of proline hydroxylation sites, amino acid motif and bias analyses, efficient communication with web servers for prediction of N-terminal signal peptides, glycosylphosphatidylinositol modification sites and disordered regions and the ability to annotate sequences through hmmscan and subsequent GO enrichment, based on predicted Pfam domains.
31508799	6	17	theme	sequence	1319:1326	arg1	analyses					1333:1340	high-throughput sequence data analyses	1303:1340	high-throughput sequence data analyses	1303:1340	As such, ragp extends R's rich ecosystem for high-throughput sequence data analyses.
31508799	2	18	theme	prototypical	615:626	arg1	sequences					628:636	prototypical sequences	615:636	prototypical sequences	615:636	Current approaches, based on identifying sequences with characteristic motifs and biased amino acid composition, are limited to prototypical sequences.
31508799	7	19	theme	MIT	1385:1387	arg1	Source					1394:1399	the MIT Open Source license	1381:1407	the MIT Open Source license	1381:1407	The ragp R package is available under the MIT Open Source license and is freely available to download from GitHub at: https://github.com/missuse/ragp.
31508799	5	20	theme	modification	1099:1110	arg1	sites					1112:1116	glycosylphosphatidylinositol modification sites	1070:1116	glycosylphosphatidylinositol modification sites	1070:1116	Main package features include prediction of proline hydroxylation sites, amino acid motif and bias analyses, efficient communication with web servers for prediction of N-terminal signal peptides, glycosylphosphatidylinositol modification sites and disordered regions and the ability to annotate sequences through hmmscan and subsequent GO enrichment, based on predicted Pfam domains.
31508799	5	20	theme	modification	1099:1110	arg1	peptides					1060:1067	N-terminal signal peptides	1042:1067	N-terminal signal peptides	1042:1067	Main package features include prediction of proline hydroxylation sites, amino acid motif and bias analyses, efficient communication with web servers for prediction of N-terminal signal peptides, glycosylphosphatidylinositol modification sites and disordered regions and the ability to annotate sequences through hmmscan and subsequent GO enrichment, based on predicted Pfam domains.
31508799	2	21	theme	Current	487:493	arg1	approaches					495:504	Current approaches	487:504	Current approaches	487:504	Current approaches, based on identifying sequences with characteristic motifs and biased amino acid composition, are limited to prototypical sequences.
31508799	3	22	theme	HRGPs	687:691	arg1	analysis					675:682	analysis	675:682	analysis of HRGPs	675:691	Ragp is an R package for mining and analysis of HRGPs, with emphasis on arabinogalactan proteins.
31508799	3	22	theme	HRGPs	687:691	arg1	mining					664:669	mining	664:669	mining	664:669	Ragp is an R package for mining and analysis of HRGPs, with emphasis on arabinogalactan proteins.
31508799	4	23	theme	filtering	746:754	arg1	pipeline					756:763	The ragp filtering pipeline	737:763	The ragp filtering pipeline	737:763	The ragp filtering pipeline exploits one of the HRGPs key features, the presence of hydroxyprolines which represent glycosylation sites.
31508799	3	24	with	package	652:658	arg1	emphasis					699:706	emphasis	699:706	emphasis on arabinogalactan proteins	699:734	Ragp is an R package for mining and analysis of HRGPs, with emphasis on arabinogalactan proteins.
31508799	7	25	theme	R	1352:1352	arg1	available					1365:1373	available	1365:1373	available	1365:1373	The ragp R package is available under the MIT Open Source license and is freely available to download from GitHub at: https://github.com/missuse/ragp.
31508799	7	25	theme	R	1352:1352	arg1	//github.com/missuse/ragp					1467:1491	//github.com/missuse/ragp	1467:1491	//github.com/missuse/ragp	1467:1491	The ragp R package is available under the MIT Open Source license and is freely available to download from GitHub at: https://github.com/missuse/ragp.
31508799	7	25	theme	R	1352:1352	arg1	package					1354:1360	The ragp R package	1343:1360	The ragp R package	1343:1360	The ragp R package is available under the MIT Open Source license and is freely available to download from GitHub at: https://github.com/missuse/ragp.
31508799	7	25	theme	R	1352:1352	arg1	available					1423:1431	available	1423:1431	available	1423:1431	The ragp R package is available under the MIT Open Source license and is freely available to download from GitHub at: https://github.com/missuse/ragp.
31508799	7	26	from	GitHub	1450:1455	arg1	download					1436:1443	download	1436:1443	download from GitHub	1436:1455	The ragp R package is available under the MIT Open Source license and is freely available to download from GitHub at: https://github.com/missuse/ragp.
31508799	0	27	theme	due	209:211	arg1	plants					201:206	plants	201:206	plants	201:206	ragp: Pipeline for mining of plant hydroxyproline-rich glycoproteins with implementation in R. Hydroxyproline-rich glycoproteins (HRGPs) are one of the most complex families of macromolecules found in plants, due to the diversity of glycans decorating the protein backbone, as well as the heterogeneity of the protein backbones.
31508799	0	28	theme	glycans	233:239	arg1	diversity					220:228	the diversity	216:228	the diversity of glycans decorating the protein backbone, as well as the heterogeneity of the protein backbones	216:326	ragp: Pipeline for mining of plant hydroxyproline-rich glycoproteins with implementation in R. Hydroxyproline-rich glycoproteins (HRGPs) are one of the most complex families of macromolecules found in plants, due to the diversity of glycans decorating the protein backbone, as well as the heterogeneity of the protein backbones.
31508799	5	29	theme	hydroxylation	926:938	arg1	sites					940:944	proline hydroxylation sites	918:944	proline hydroxylation sites	918:944	Main package features include prediction of proline hydroxylation sites, amino acid motif and bias analyses, efficient communication with web servers for prediction of N-terminal signal peptides, glycosylphosphatidylinositol modification sites and disordered regions and the ability to annotate sequences through hmmscan and subsequent GO enrichment, based on predicted Pfam domains.
31508799	0	30	theme	backbones	318:326	arg1	backbone					264:271	the protein backbone	252:271	the protein backbone	252:271	ragp: Pipeline for mining of plant hydroxyproline-rich glycoproteins with implementation in R. Hydroxyproline-rich glycoproteins (HRGPs) are one of the most complex families of macromolecules found in plants, due to the diversity of glycans decorating the protein backbone, as well as the heterogeneity of the protein backbones.
31508799	0	30	theme	backbones	318:326	arg1	heterogeneity					289:301	the heterogeneity	285:301	the heterogeneity of the protein backbones	285:326	ragp: Pipeline for mining of plant hydroxyproline-rich glycoproteins with implementation in R. Hydroxyproline-rich glycoproteins (HRGPs) are one of the most complex families of macromolecules found in plants, due to the diversity of glycans decorating the protein backbone, as well as the heterogeneity of the protein backbones.
31508799	4	31	gly	glycosylation	853:865	arg2	sites					867:871	glycosylation sites	853:871	glycosylation sites	853:871	The ragp filtering pipeline exploits one of the HRGPs key features, the presence of hydroxyprolines which represent glycosylation sites.
31508799	3	32	theme	R	650:650	arg1	Ragp					639:642	Ragp	639:642	Ragp	639:642	Ragp is an R package for mining and analysis of HRGPs, with emphasis on arabinogalactan proteins.
31508799	3	32	theme	R	650:650	arg1	package					652:658	an R package	647:658	an R package for mining and analysis of HRGPs	647:691	Ragp is an R package for mining and analysis of HRGPs, with emphasis on arabinogalactan proteins.
31508799	0	33	theme	protein	256:262	arg1	backbone					264:271	the protein backbone	252:271	the protein backbone	252:271	ragp: Pipeline for mining of plant hydroxyproline-rich glycoproteins with implementation in R. Hydroxyproline-rich glycoproteins (HRGPs) are one of the most complex families of macromolecules found in plants, due to the diversity of glycans decorating the protein backbone, as well as the heterogeneity of the protein backbones.
31508799	5	34	theme	sites	940:944	arg1	prediction					904:913	prediction	904:913	prediction of proline hydroxylation sites, amino acid motif and bias analyses	904:980	Main package features include prediction of proline hydroxylation sites, amino acid motif and bias analyses, efficient communication with web servers for prediction of N-terminal signal peptides, glycosylphosphatidylinositol modification sites and disordered regions and the ability to annotate sequences through hmmscan and subsequent GO enrichment, based on predicted Pfam domains.
31508799	5	34	theme	sites	940:944	arg1	communication					993:1005	efficient communication	983:1005	efficient communication with web servers for prediction of N-terminal signal peptides, glycosylphosphatidylinositol modification sites and disordered regions	983:1139	Main package features include prediction of proline hydroxylation sites, amino acid motif and bias analyses, efficient communication with web servers for prediction of N-terminal signal peptides, glycosylphosphatidylinositol modification sites and disordered regions and the ability to annotate sequences through hmmscan and subsequent GO enrichment, based on predicted Pfam domains.
31508799	5	34	theme	sites	940:944	arg1	ability					1149:1155	the ability	1145:1155	the ability to annotate sequences through hmmscan and subsequent GO enrichment, based on predicted Pfam domains	1145:1255	Main package features include prediction of proline hydroxylation sites, amino acid motif and bias analyses, efficient communication with web servers for prediction of N-terminal signal peptides, glycosylphosphatidylinositol modification sites and disordered regions and the ability to annotate sequences through hmmscan and subsequent GO enrichment, based on predicted Pfam domains.
31508799	7	35	dep	Source	1394:1399	arg1	license					1401:1407	license	1401:1407	license	1401:1407	The ragp R package is available under the MIT Open Source license and is freely available to download from GitHub at: https://github.com/missuse/ragp.
31508799	5	36	theme	subsequent	1199:1208	arg1	enrichment					1213:1222	subsequent GO enrichment	1199:1222	subsequent GO enrichment	1199:1222	Main package features include prediction of proline hydroxylation sites, amino acid motif and bias analyses, efficient communication with web servers for prediction of N-terminal signal peptides, glycosylphosphatidylinositol modification sites and disordered regions and the ability to annotate sequences through hmmscan and subsequent GO enrichment, based on predicted Pfam domains.
31508799	2	37	theme	characteristic	543:556	arg1	motifs					558:563	characteristic motifs	543:563	characteristic motifs	543:563	Current approaches, based on identifying sequences with characteristic motifs and biased amino acid composition, are limited to prototypical sequences.
31508799	0	38	gly	glycoproteins	115:127	arg1	HRGPs					130:134	HRGPs	130:134	HRGPs	130:134	ragp: Pipeline for mining of plant hydroxyproline-rich glycoproteins with implementation in R. Hydroxyproline-rich glycoproteins (HRGPs) are one of the most complex families of macromolecules found in plants, due to the diversity of glycans decorating the protein backbone, as well as the heterogeneity of the protein backbones.
31508799	0	38	gly	glycoproteins	115:127	arg1	glycoproteins					115:127	R. Hydroxyproline-rich glycoproteins	92:127	R. Hydroxyproline-rich glycoproteins (HRGPs)	92:135	ragp: Pipeline for mining of plant hydroxyproline-rich glycoproteins with implementation in R. Hydroxyproline-rich glycoproteins (HRGPs) are one of the most complex families of macromolecules found in plants, due to the diversity of glycans decorating the protein backbone, as well as the heterogeneity of the protein backbones.
31508799	7	39	theme	Open	1389:1392	arg1	Source					1394:1399	the MIT Open Source license	1381:1407	the MIT Open Source license	1381:1407	The ragp R package is available under the MIT Open Source license and is freely available to download from GitHub at: https://github.com/missuse/ragp.
31508799	5	40	theme	acid	953:956	arg1	motif					958:962	amino acid motif	947:962	amino acid motif	947:962	Main package features include prediction of proline hydroxylation sites, amino acid motif and bias analyses, efficient communication with web servers for prediction of N-terminal signal peptides, glycosylphosphatidylinositol modification sites and disordered regions and the ability to annotate sequences through hmmscan and subsequent GO enrichment, based on predicted Pfam domains.
31508799	3	41	from	emphasis	699:706	arg1	proteins					727:734	arabinogalactan proteins	711:734	arabinogalactan proteins	711:734	Ragp is an R package for mining and analysis of HRGPs, with emphasis on arabinogalactan proteins.
31508799	5	42	theme	peptides	1060:1067	arg1	prediction					1028:1037	prediction	1028:1037	prediction of N-terminal signal peptides, glycosylphosphatidylinositol modification sites and disordered regions	1028:1139	Main package features include prediction of proline hydroxylation sites, amino acid motif and bias analyses, efficient communication with web servers for prediction of N-terminal signal peptides, glycosylphosphatidylinositol modification sites and disordered regions and the ability to annotate sequences through hmmscan and subsequent GO enrichment, based on predicted Pfam domains.
31508799	5	43	theme	amino	947:951	arg1	motif					958:962	amino acid motif	947:962	amino acid motif	947:962	Main package features include prediction of proline hydroxylation sites, amino acid motif and bias analyses, efficient communication with web servers for prediction of N-terminal signal peptides, glycosylphosphatidylinositol modification sites and disordered regions and the ability to annotate sequences through hmmscan and subsequent GO enrichment, based on predicted Pfam domains.
31508799	3	44	theme	arabinogalactan	711:725	arg1	proteins					727:734	arabinogalactan proteins	711:734	arabinogalactan proteins	711:734	Ragp is an R package for mining and analysis of HRGPs, with emphasis on arabinogalactan proteins.
31508799	2	45	theme	identifying	516:526	arg1	sequences					528:536	identifying sequences	516:536	identifying sequences with characteristic motifs and biased amino acid composition	516:597	Current approaches, based on identifying sequences with characteristic motifs and biased amino acid composition, are limited to prototypical sequences.
31508799	0	46	from	implementation	74:87	arg1	HRGPs					130:134	HRGPs	130:134	HRGPs	130:134	ragp: Pipeline for mining of plant hydroxyproline-rich glycoproteins with implementation in R. Hydroxyproline-rich glycoproteins (HRGPs) are one of the most complex families of macromolecules found in plants, due to the diversity of glycans decorating the protein backbone, as well as the heterogeneity of the protein backbones.
31508799	0	46	from	implementation	74:87	arg1	glycoproteins					115:127	R. Hydroxyproline-rich glycoproteins	92:127	R. Hydroxyproline-rich glycoproteins (HRGPs)	92:135	ragp: Pipeline for mining of plant hydroxyproline-rich glycoproteins with implementation in R. Hydroxyproline-rich glycoproteins (HRGPs) are one of the most complex families of macromolecules found in plants, due to the diversity of glycans decorating the protein backbone, as well as the heterogeneity of the protein backbones.
31508799	4	47	theme	key	791:793	arg1	features					795:802	the HRGPs key features	781:802	the HRGPs key features	781:802	The ragp filtering pipeline exploits one of the HRGPs key features, the presence of hydroxyprolines which represent glycosylation sites.
31508799	4	47	theme	key	791:793	arg1	presence					809:816	the presence	805:816	the presence of hydroxyprolines which represent glycosylation sites	805:871	The ragp filtering pipeline exploits one of the HRGPs key features, the presence of hydroxyprolines which represent glycosylation sites.
31508799	2	48	with	sequences	528:536	arg1	composition					587:597	biased amino acid composition	569:597	biased amino acid composition	569:597	Current approaches, based on identifying sequences with characteristic motifs and biased amino acid composition, are limited to prototypical sequences.
31508799	2	48	with	sequences	528:536	arg1	motifs					558:563	characteristic motifs	543:563	characteristic motifs	543:563	Current approaches, based on identifying sequences with characteristic motifs and biased amino acid composition, are limited to prototypical sequences.
31508799	7	49	dep	available	1365:1373	arg1	available					1365:1373	available	1365:1373	available	1365:1373	The ragp R package is available under the MIT Open Source license and is freely available to download from GitHub at: https://github.com/missuse/ragp.
31508799	7	49	dep	available	1365:1373	arg1	//github.com/missuse/ragp					1467:1491	//github.com/missuse/ragp	1467:1491	//github.com/missuse/ragp	1467:1491	The ragp R package is available under the MIT Open Source license and is freely available to download from GitHub at: https://github.com/missuse/ragp.
31508799	7	49	dep	available	1365:1373	arg1	package					1354:1360	The ragp R package	1343:1360	The ragp R package	1343:1360	The ragp R package is available under the MIT Open Source license and is freely available to download from GitHub at: https://github.com/missuse/ragp.
31508799	7	49	dep	available	1365:1373	arg1	available					1423:1431	available	1423:1431	available	1423:1431	The ragp R package is available under the MIT Open Source license and is freely available to download from GitHub at: https://github.com/missuse/ragp.
31508799	7	50	theme	ragp	1347:1350	arg1	available					1365:1373	available	1365:1373	available	1365:1373	The ragp R package is available under the MIT Open Source license and is freely available to download from GitHub at: https://github.com/missuse/ragp.
31508799	7	50	theme	ragp	1347:1350	arg1	//github.com/missuse/ragp					1467:1491	//github.com/missuse/ragp	1467:1491	//github.com/missuse/ragp	1467:1491	The ragp R package is available under the MIT Open Source license and is freely available to download from GitHub at: https://github.com/missuse/ragp.
31508799	7	50	theme	ragp	1347:1350	arg1	package					1354:1360	The ragp R package	1343:1360	The ragp R package	1343:1360	The ragp R package is available under the MIT Open Source license and is freely available to download from GitHub at: https://github.com/missuse/ragp.
31508799	7	50	theme	ragp	1347:1350	arg1	available					1423:1431	available	1423:1431	available	1423:1431	The ragp R package is available under the MIT Open Source license and is freely available to download from GitHub at: https://github.com/missuse/ragp.
31508799	4	51	theme	hydroxyprolines	821:835	arg1	features					795:802	the HRGPs key features	781:802	the HRGPs key features	781:802	The ragp filtering pipeline exploits one of the HRGPs key features, the presence of hydroxyprolines which represent glycosylation sites.
31508799	4	51	theme	hydroxyprolines	821:835	arg1	presence					809:816	the presence	805:816	the presence of hydroxyprolines which represent glycosylation sites	805:871	The ragp filtering pipeline exploits one of the HRGPs key features, the presence of hydroxyprolines which represent glycosylation sites.
31508799	5	52	theme	glycosylphosphatidylinositol	1070:1097	arg1	sites					1112:1116	glycosylphosphatidylinositol modification sites	1070:1116	glycosylphosphatidylinositol modification sites	1070:1116	Main package features include prediction of proline hydroxylation sites, amino acid motif and bias analyses, efficient communication with web servers for prediction of N-terminal signal peptides, glycosylphosphatidylinositol modification sites and disordered regions and the ability to annotate sequences through hmmscan and subsequent GO enrichment, based on predicted Pfam domains.
31508799	5	52	theme	glycosylphosphatidylinositol	1070:1097	arg1	peptides					1060:1067	N-terminal signal peptides	1042:1067	N-terminal signal peptides	1042:1067	Main package features include prediction of proline hydroxylation sites, amino acid motif and bias analyses, efficient communication with web servers for prediction of N-terminal signal peptides, glycosylphosphatidylinositol modification sites and disordered regions and the ability to annotate sequences through hmmscan and subsequent GO enrichment, based on predicted Pfam domains.
31508799	4	53	theme	HRGPs	785:789	arg1	features					795:802	the HRGPs key features	781:802	the HRGPs key features	781:802	The ragp filtering pipeline exploits one of the HRGPs key features, the presence of hydroxyprolines which represent glycosylation sites.
31508799	4	53	theme	HRGPs	785:789	arg1	presence					809:816	the presence	805:816	the presence of hydroxyprolines which represent glycosylation sites	805:871	The ragp filtering pipeline exploits one of the HRGPs key features, the presence of hydroxyprolines which represent glycosylation sites.
31508799	1	54	theme	homology	456:463	arg1	identification					471:484	homology based identification	456:484	homology based identification	456:484	While this diversity is responsible for a wide array of physiological functions associated with HRGPs, it hinders attempts for homology based identification.
31508799	6	55	theme	high-throughput	1303:1317	arg1	analyses					1333:1340	high-throughput sequence data analyses	1303:1340	high-throughput sequence data analyses	1303:1340	As such, ragp extends R's rich ecosystem for high-throughput sequence data analyses.
31508799	0	56	theme	hydroxyproline-rich	35:53	arg1	glycoproteins					55:67	plant hydroxyproline-rich glycoproteins	29:67	plant hydroxyproline-rich glycoproteins	29:67	ragp: Pipeline for mining of plant hydroxyproline-rich glycoproteins with implementation in R. Hydroxyproline-rich glycoproteins (HRGPs) are one of the most complex families of macromolecules found in plants, due to the diversity of glycans decorating the protein backbone, as well as the heterogeneity of the protein backbones.
31508799	2	57	theme	biased	569:574	arg1	composition					587:597	biased amino acid composition	569:597	biased amino acid composition	569:597	Current approaches, based on identifying sequences with characteristic motifs and biased amino acid composition, are limited to prototypical sequences.
31508799	1	58	theme	wide	371:374	arg1	array					376:380	a wide array	369:380	a wide array of physiological functions associated with HRGPs	369:429	While this diversity is responsible for a wide array of physiological functions associated with HRGPs, it hinders attempts for homology based identification.
31508799	1	59	theme	based	465:469	arg1	identification					471:484	homology based identification	456:484	homology based identification	456:484	While this diversity is responsible for a wide array of physiological functions associated with HRGPs, it hinders attempts for homology based identification.
31508799	0	60	theme	plant	29:33	arg1	glycoproteins					55:67	plant hydroxyproline-rich glycoproteins	29:67	plant hydroxyproline-rich glycoproteins	29:67	ragp: Pipeline for mining of plant hydroxyproline-rich glycoproteins with implementation in R. Hydroxyproline-rich glycoproteins (HRGPs) are one of the most complex families of macromolecules found in plants, due to the diversity of glycans decorating the protein backbone, as well as the heterogeneity of the protein backbones.
31508799	0	61	gly	heterogeneity	289:301	arg1	backbones					318:326	the protein backbones	306:326	the protein backbones	306:326	ragp: Pipeline for mining of plant hydroxyproline-rich glycoproteins with implementation in R. Hydroxyproline-rich glycoproteins (HRGPs) are one of the most complex families of macromolecules found in plants, due to the diversity of glycans decorating the protein backbone, as well as the heterogeneity of the protein backbones.
31508799	5	62	with	prediction	904:913	arg1	servers					1016:1022	web servers	1012:1022	web servers for prediction of N-terminal signal peptides, glycosylphosphatidylinositol modification sites and disordered regions	1012:1139	Main package features include prediction of proline hydroxylation sites, amino acid motif and bias analyses, efficient communication with web servers for prediction of N-terminal signal peptides, glycosylphosphatidylinositol modification sites and disordered regions and the ability to annotate sequences through hmmscan and subsequent GO enrichment, based on predicted Pfam domains.
31508799	4	63	theme	features	795:802	arg1	features					795:802	the HRGPs key features	781:802	the HRGPs key features	781:802	The ragp filtering pipeline exploits one of the HRGPs key features, the presence of hydroxyprolines which represent glycosylation sites.
31508799	4	63	theme	features	795:802	arg1	one					774:776	one	774:776	one	774:776	The ragp filtering pipeline exploits one of the HRGPs key features, the presence of hydroxyprolines which represent glycosylation sites.
31508799	4	63	theme	features	795:802	arg1	presence					809:816	the presence	805:816	the presence of hydroxyprolines which represent glycosylation sites	805:871	The ragp filtering pipeline exploits one of the HRGPs key features, the presence of hydroxyprolines which represent glycosylation sites.
31508799	6	64	theme	rich	1284:1287	arg1	ecosystem					1289:1297	R's rich ecosystem	1280:1297	R's rich ecosystem for high-throughput sequence data analyses	1280:1340	As such, ragp extends R's rich ecosystem for high-throughput sequence data analyses.
31508799	5	65	with	communication	993:1005	arg1	servers					1016:1022	web servers	1012:1022	web servers for prediction of N-terminal signal peptides, glycosylphosphatidylinositol modification sites and disordered regions	1012:1139	Main package features include prediction of proline hydroxylation sites, amino acid motif and bias analyses, efficient communication with web servers for prediction of N-terminal signal peptides, glycosylphosphatidylinositol modification sites and disordered regions and the ability to annotate sequences through hmmscan and subsequent GO enrichment, based on predicted Pfam domains.
31508799	0	66	theme	complex	157:163	arg1	families					165:172	the most complex families	148:172	the most complex families of macromolecules found in plants, due to the diversity of glycans decorating the protein backbone, as well as the heterogeneity of the protein backbones	148:326	ragp: Pipeline for mining of plant hydroxyproline-rich glycoproteins with implementation in R. Hydroxyproline-rich glycoproteins (HRGPs) are one of the most complex families of macromolecules found in plants, due to the diversity of glycans decorating the protein backbone, as well as the heterogeneity of the protein backbones.
31508799	5	67	theme	analyses	973:980	arg1	prediction					904:913	prediction	904:913	prediction of proline hydroxylation sites, amino acid motif and bias analyses	904:980	Main package features include prediction of proline hydroxylation sites, amino acid motif and bias analyses, efficient communication with web servers for prediction of N-terminal signal peptides, glycosylphosphatidylinositol modification sites and disordered regions and the ability to annotate sequences through hmmscan and subsequent GO enrichment, based on predicted Pfam domains.
31508799	5	67	theme	analyses	973:980	arg1	communication					993:1005	efficient communication	983:1005	efficient communication with web servers for prediction of N-terminal signal peptides, glycosylphosphatidylinositol modification sites and disordered regions	983:1139	Main package features include prediction of proline hydroxylation sites, amino acid motif and bias analyses, efficient communication with web servers for prediction of N-terminal signal peptides, glycosylphosphatidylinositol modification sites and disordered regions and the ability to annotate sequences through hmmscan and subsequent GO enrichment, based on predicted Pfam domains.
31508799	5	67	theme	analyses	973:980	arg1	ability					1149:1155	the ability	1145:1155	the ability to annotate sequences through hmmscan and subsequent GO enrichment, based on predicted Pfam domains	1145:1255	Main package features include prediction of proline hydroxylation sites, amino acid motif and bias analyses, efficient communication with web servers for prediction of N-terminal signal peptides, glycosylphosphatidylinositol modification sites and disordered regions and the ability to annotate sequences through hmmscan and subsequent GO enrichment, based on predicted Pfam domains.
31508799	5	68	theme	web	1012:1014	arg1	servers					1016:1022	web servers	1012:1022	web servers for prediction of N-terminal signal peptides, glycosylphosphatidylinositol modification sites and disordered regions	1012:1139	Main package features include prediction of proline hydroxylation sites, amino acid motif and bias analyses, efficient communication with web servers for prediction of N-terminal signal peptides, glycosylphosphatidylinositol modification sites and disordered regions and the ability to annotate sequences through hmmscan and subsequent GO enrichment, based on predicted Pfam domains.
31508799	5	69	theme	Pfam	1244:1247	arg1	domains					1249:1255	predicted Pfam domains	1234:1255	predicted Pfam domains	1234:1255	Main package features include prediction of proline hydroxylation sites, amino acid motif and bias analyses, efficient communication with web servers for prediction of N-terminal signal peptides, glycosylphosphatidylinositol modification sites and disordered regions and the ability to annotate sequences through hmmscan and subsequent GO enrichment, based on predicted Pfam domains.
31508799	0	70	theme	most	152:155	arg1	families					165:172	the most complex families	148:172	the most complex families of macromolecules found in plants, due to the diversity of glycans decorating the protein backbone, as well as the heterogeneity of the protein backbones	148:326	ragp: Pipeline for mining of plant hydroxyproline-rich glycoproteins with implementation in R. Hydroxyproline-rich glycoproteins (HRGPs) are one of the most complex families of macromolecules found in plants, due to the diversity of glycans decorating the protein backbone, as well as the heterogeneity of the protein backbones.
31508799	1	71	theme	physiological	385:397	arg1	functions					399:407	physiological functions	385:407	physiological functions associated with HRGPs	385:429	While this diversity is responsible for a wide array of physiological functions associated with HRGPs, it hinders attempts for homology based identification.
31508799	4	72	theme	ragp	741:744	arg1	pipeline					756:763	The ragp filtering pipeline	737:763	The ragp filtering pipeline	737:763	The ragp filtering pipeline exploits one of the HRGPs key features, the presence of hydroxyprolines which represent glycosylation sites.
31508799	5	73	theme	proline	918:924	arg1	sites					940:944	proline hydroxylation sites	918:944	proline hydroxylation sites	918:944	Main package features include prediction of proline hydroxylation sites, amino acid motif and bias analyses, efficient communication with web servers for prediction of N-terminal signal peptides, glycosylphosphatidylinositol modification sites and disordered regions and the ability to annotate sequences through hmmscan and subsequent GO enrichment, based on predicted Pfam domains.
31508799	4	74	theme	glycosylation	853:865	arg1	sites					867:871	glycosylation sites	853:871	glycosylation sites	853:871	The ragp filtering pipeline exploits one of the HRGPs key features, the presence of hydroxyprolines which represent glycosylation sites.
31508799	5	75	theme	N-terminal	1042:1051	arg1	sites					1112:1116	glycosylphosphatidylinositol modification sites	1070:1116	glycosylphosphatidylinositol modification sites	1070:1116	Main package features include prediction of proline hydroxylation sites, amino acid motif and bias analyses, efficient communication with web servers for prediction of N-terminal signal peptides, glycosylphosphatidylinositol modification sites and disordered regions and the ability to annotate sequences through hmmscan and subsequent GO enrichment, based on predicted Pfam domains.
31508799	5	75	theme	N-terminal	1042:1051	arg1	regions					1133:1139	disordered regions	1122:1139	disordered regions	1122:1139	Main package features include prediction of proline hydroxylation sites, amino acid motif and bias analyses, efficient communication with web servers for prediction of N-terminal signal peptides, glycosylphosphatidylinositol modification sites and disordered regions and the ability to annotate sequences through hmmscan and subsequent GO enrichment, based on predicted Pfam domains.
31508799	5	75	theme	N-terminal	1042:1051	arg1	peptides					1060:1067	N-terminal signal peptides	1042:1067	N-terminal signal peptides	1042:1067	Main package features include prediction of proline hydroxylation sites, amino acid motif and bias analyses, efficient communication with web servers for prediction of N-terminal signal peptides, glycosylphosphatidylinositol modification sites and disordered regions and the ability to annotate sequences through hmmscan and subsequent GO enrichment, based on predicted Pfam domains.
31508799	1	76	theme	functions	399:407	arg1	array					376:380	a wide array	369:380	a wide array of physiological functions associated with HRGPs	369:429	While this diversity is responsible for a wide array of physiological functions associated with HRGPs, it hinders attempts for homology based identification.
31508799	5	77	theme	efficient	983:991	arg1	communication					993:1005	efficient communication	983:1005	efficient communication with web servers for prediction of N-terminal signal peptides, glycosylphosphatidylinositol modification sites and disordered regions	983:1139	Main package features include prediction of proline hydroxylation sites, amino acid motif and bias analyses, efficient communication with web servers for prediction of N-terminal signal peptides, glycosylphosphatidylinositol modification sites and disordered regions and the ability to annotate sequences through hmmscan and subsequent GO enrichment, based on predicted Pfam domains.
31508799	0	78	located	found	192:196	arg1	plants					201:206	plants	201:206	plants	201:206	ragp: Pipeline for mining of plant hydroxyproline-rich glycoproteins with implementation in R. Hydroxyproline-rich glycoproteins (HRGPs) are one of the most complex families of macromolecules found in plants, due to the diversity of glycans decorating the protein backbone, as well as the heterogeneity of the protein backbones.
31508799	0	78	located	found	192:196	arg2	macromolecules					177:190	macromolecules	177:190	macromolecules found in plants, due to the diversity of glycans decorating the protein backbone, as well as the heterogeneity of the protein backbones	177:326	ragp: Pipeline for mining of plant hydroxyproline-rich glycoproteins with implementation in R. Hydroxyproline-rich glycoproteins (HRGPs) are one of the most complex families of macromolecules found in plants, due to the diversity of glycans decorating the protein backbone, as well as the heterogeneity of the protein backbones.
31508799	0	79	theme	glycoproteins	55:67	arg1	mining					19:24	mining	19:24	mining of plant hydroxyproline-rich glycoproteins with implementation in R. Hydroxyproline-rich glycoproteins (HRGPs)	19:135	ragp: Pipeline for mining of plant hydroxyproline-rich glycoproteins with implementation in R. Hydroxyproline-rich glycoproteins (HRGPs) are one of the most complex families of macromolecules found in plants, due to the diversity of glycans decorating the protein backbone, as well as the heterogeneity of the protein backbones.
31508799	7	80	from	https	1461:1465	arg1	available					1365:1373	available	1365:1373	available	1365:1373	The ragp R package is available under the MIT Open Source license and is freely available to download from GitHub at: https://github.com/missuse/ragp.
31508799	7	80	from	https	1461:1465	arg1	//github.com/missuse/ragp					1467:1491	//github.com/missuse/ragp	1467:1491	//github.com/missuse/ragp	1467:1491	The ragp R package is available under the MIT Open Source license and is freely available to download from GitHub at: https://github.com/missuse/ragp.
31508799	7	80	from	https	1461:1465	arg1	package					1354:1360	The ragp R package	1343:1360	The ragp R package	1343:1360	The ragp R package is available under the MIT Open Source license and is freely available to download from GitHub at: https://github.com/missuse/ragp.
31508799	7	80	from	https	1461:1465	arg1	available					1423:1431	available	1423:1431	available	1423:1431	The ragp R package is available under the MIT Open Source license and is freely available to download from GitHub at: https://github.com/missuse/ragp.
31508799	5	81	theme	disordered	1122:1131	arg1	regions					1133:1139	disordered regions	1122:1139	disordered regions	1122:1139	Main package features include prediction of proline hydroxylation sites, amino acid motif and bias analyses, efficient communication with web servers for prediction of N-terminal signal peptides, glycosylphosphatidylinositol modification sites and disordered regions and the ability to annotate sequences through hmmscan and subsequent GO enrichment, based on predicted Pfam domains.
31508799	5	81	theme	disordered	1122:1131	arg1	peptides					1060:1067	N-terminal signal peptides	1042:1067	N-terminal signal peptides	1042:1067	Main package features include prediction of proline hydroxylation sites, amino acid motif and bias analyses, efficient communication with web servers for prediction of N-terminal signal peptides, glycosylphosphatidylinositol modification sites and disordered regions and the ability to annotate sequences through hmmscan and subsequent GO enrichment, based on predicted Pfam domains.
31508799	5	82	theme	Main	874:877	arg1	features					887:894	Main package features	874:894	Main package features	874:894	Main package features include prediction of proline hydroxylation sites, amino acid motif and bias analyses, efficient communication with web servers for prediction of N-terminal signal peptides, glycosylphosphatidylinositol modification sites and disordered regions and the ability to annotate sequences through hmmscan and subsequent GO enrichment, based on predicted Pfam domains.
31508799	5	83	with	ability	1149:1155	arg1	servers					1016:1022	web servers	1012:1022	web servers for prediction of N-terminal signal peptides, glycosylphosphatidylinositol modification sites and disordered regions	1012:1139	Main package features include prediction of proline hydroxylation sites, amino acid motif and bias analyses, efficient communication with web servers for prediction of N-terminal signal peptides, glycosylphosphatidylinositol modification sites and disordered regions and the ability to annotate sequences through hmmscan and subsequent GO enrichment, based on predicted Pfam domains.
30077759	11	0	theme	promising	1669:1677	arg1	formulation					1648:1658	the optimized CCMD formulation	1629:1658	the optimized CCMD formulation	1629:1658	Consequently, the optimized CCMD formulation can be a promising, safe and efficient platform for the transcorneal delivery of lipophilic drugs including most antifungals.
30077759	11	0	theme	promising	1669:1677	arg1	platform					1699:1706	a promising, safe and efficient platform	1667:1706	a promising, safe and efficient platform for the transcorneal delivery of lipophilic drugs including most antifungals	1667:1783	Consequently, the optimized CCMD formulation can be a promising, safe and efficient platform for the transcorneal delivery of lipophilic drugs including most antifungals.
30077759	7	1	theme	polyethylene	1143:1154	arg1	oxide					1156:1160	0.02% polyethylene oxide	1137:1160	0.02% polyethylene oxide	1137:1160	The optimum formula was chosen based on a factorial response surface analysis and it was composed of 17:1 w/w surfactant/drug, 30:1 w/w cyclodextrin/drug ratios and 0.02% polyethylene oxide.
30077759	11	2	theme	CCMD	1643:1646	arg1	formulation					1648:1658	the optimized CCMD formulation	1629:1658	the optimized CCMD formulation	1629:1658	Consequently, the optimized CCMD formulation can be a promising, safe and efficient platform for the transcorneal delivery of lipophilic drugs including most antifungals.
30077759	11	2	theme	CCMD	1643:1646	arg1	platform					1699:1706	a promising, safe and efficient platform	1667:1706	a promising, safe and efficient platform for the transcorneal delivery of lipophilic drugs including most antifungals	1667:1783	Consequently, the optimized CCMD formulation can be a promising, safe and efficient platform for the transcorneal delivery of lipophilic drugs including most antifungals.
30077759	1	3	theme	delivery	176:183	arg1	system					185:190	efficient ocular drug delivery system	154:190	efficient ocular drug delivery system for antifungal drugs	154:211	Development of efficient ocular drug delivery system for antifungal drugs becomes a must nowadays to face and eradicate the widely spread ophthalmic fungal infections.
30077759	6	4	theme	potential	931:939	arg1	analysis					893:900	the analysis	889:900	the analysis of their particle size, zeta potential and solubilization efficiency	889:969	The prepared CCMD were characterized through the analysis of their particle size, zeta potential and solubilization efficiency.
30077759	8	5	dep	in	1193:1194	arg1	vitro					1196:1200	vitro	1196:1200	vitro	1196:1200	This formula was subjected to in vitro characterization including release, imaging by transmission electron microscope, mucoadhesion, stability, in addition to the determination of the minimum inhibitory concentration.
30077759	7	6	theme	cyclodextrin/drug	1108:1124	arg1	ratios					1126:1131	30:1 w/w cyclodextrin/drug ratios	1099:1131	30:1 w/w cyclodextrin/drug ratios	1099:1131	The optimum formula was chosen based on a factorial response surface analysis and it was composed of 17:1 w/w surfactant/drug, 30:1 w/w cyclodextrin/drug ratios and 0.02% polyethylene oxide.
30077759	1	7	theme	system	185:190	arg1	Development					139:149	Development	139:149	Development of efficient ocular drug delivery system for antifungal drugs	139:211	Development of efficient ocular drug delivery system for antifungal drugs becomes a must nowadays to face and eradicate the widely spread ophthalmic fungal infections.
30077759	1	8	theme	spread	270:275	arg1	infections					295:304	the widely spread ophthalmic fungal infections	259:304	the widely spread ophthalmic fungal infections	259:304	Development of efficient ocular drug delivery system for antifungal drugs becomes a must nowadays to face and eradicate the widely spread ophthalmic fungal infections.
30077759	6	9	theme	zeta	926:929	arg1	potential					931:939	zeta potential	926:939	zeta potential	926:939	The prepared CCMD were characterized through the analysis of their particle size, zeta potential and solubilization efficiency.
30077759	7	10	theme	0.02	1137:1140	arg1	%					1141:1141	%	1141:1141	%	1141:1141	The optimum formula was chosen based on a factorial response surface analysis and it was composed of 17:1 w/w surfactant/drug, 30:1 w/w cyclodextrin/drug ratios and 0.02% polyethylene oxide.
30077759	8	11	theme	transmission	1249:1260	arg1	mucoadhesion					1283:1294	mucoadhesion	1283:1294	mucoadhesion	1283:1294	This formula was subjected to in vitro characterization including release, imaging by transmission electron microscope, mucoadhesion, stability, in addition to the determination of the minimum inhibitory concentration.
30077759	8	11	theme	transmission	1249:1260	arg1	microscope					1271:1280	transmission electron microscope	1249:1280	transmission electron microscope	1249:1280	This formula was subjected to in vitro characterization including release, imaging by transmission electron microscope, mucoadhesion, stability, in addition to the determination of the minimum inhibitory concentration.
30077759	8	11	theme	transmission	1249:1260	arg1	stability					1297:1305	stability	1297:1305	stability	1297:1305	This formula was subjected to in vitro characterization including release, imaging by transmission electron microscope, mucoadhesion, stability, in addition to the determination of the minimum inhibitory concentration.
30077759	3	12	theme	β-cyclodextrin	558:571	arg1	presence					546:553	presence	546:553	presence of β-cyclodextrin which acted as a dissolution and permeation enhancer	546:624	The aim of this study was to enhance itraconazole corneal penetration through utilizing the minimum surfactant amount in presence of β-cyclodextrin which acted as a dissolution and permeation enhancer.
30077759	1	13	theme	ophthalmic	277:286	arg1	infections					295:304	the widely spread ophthalmic fungal infections	259:304	the widely spread ophthalmic fungal infections	259:304	Development of efficient ocular drug delivery system for antifungal drugs becomes a must nowadays to face and eradicate the widely spread ophthalmic fungal infections.
30077759	11	14	theme	efficient	1689:1697	arg1	formulation					1648:1658	the optimized CCMD formulation	1629:1658	the optimized CCMD formulation	1629:1658	Consequently, the optimized CCMD formulation can be a promising, safe and efficient platform for the transcorneal delivery of lipophilic drugs including most antifungals.
30077759	11	14	theme	efficient	1689:1697	arg1	platform					1699:1706	a promising, safe and efficient platform	1667:1706	a promising, safe and efficient platform for the transcorneal delivery of lipophilic drugs including most antifungals	1667:1783	Consequently, the optimized CCMD formulation can be a promising, safe and efficient platform for the transcorneal delivery of lipophilic drugs including most antifungals.
30077759	7	15	theme	%	1141:1141	arg1	oxide					1156:1160	0.02% polyethylene oxide	1137:1160	0.02% polyethylene oxide	1137:1160	The optimum formula was chosen based on a factorial response surface analysis and it was composed of 17:1 w/w surfactant/drug, 30:1 w/w cyclodextrin/drug ratios and 0.02% polyethylene oxide.
30077759	3	16	from	amount	536:541	arg1	presence					546:553	presence	546:553	presence of β-cyclodextrin which acted as a dissolution and permeation enhancer	546:624	The aim of this study was to enhance itraconazole corneal penetration through utilizing the minimum surfactant amount in presence of β-cyclodextrin which acted as a dissolution and permeation enhancer.
30077759	3	17	theme	minimum	517:523	arg1	amount					536:541	the minimum surfactant amount	513:541	the minimum surfactant amount in presence of β-cyclodextrin which acted as a dissolution and permeation enhancer	513:624	The aim of this study was to enhance itraconazole corneal penetration through utilizing the minimum surfactant amount in presence of β-cyclodextrin which acted as a dissolution and permeation enhancer.
30077759	1	18	theme	fungal	288:293	arg1	infections					295:304	the widely spread ophthalmic fungal infections	259:304	the widely spread ophthalmic fungal infections	259:304	Development of efficient ocular drug delivery system for antifungal drugs becomes a must nowadays to face and eradicate the widely spread ophthalmic fungal infections.
30077759	0	19	theme	drug	133:136	arg1	permeation					95:104	the transcorneal permeation	78:104	the transcorneal permeation of a practically insoluble drug	78:136	Optimization of β-cyclodextrin consolidated micellar dispersion for promoting the transcorneal permeation of a practically insoluble drug.
30077759	6	20	theme	size	920:923	arg1	analysis					893:900	the analysis	889:900	the analysis of their particle size, zeta potential and solubilization efficiency	889:969	The prepared CCMD were characterized through the analysis of their particle size, zeta potential and solubilization efficiency.
30077759	11	21	theme	safe	1680:1683	arg1	formulation					1648:1658	the optimized CCMD formulation	1629:1658	the optimized CCMD formulation	1629:1658	Consequently, the optimized CCMD formulation can be a promising, safe and efficient platform for the transcorneal delivery of lipophilic drugs including most antifungals.
30077759	11	21	theme	safe	1680:1683	arg1	platform					1699:1706	a promising, safe and efficient platform	1667:1706	a promising, safe and efficient platform for the transcorneal delivery of lipophilic drugs including most antifungals	1667:1783	Consequently, the optimized CCMD formulation can be a promising, safe and efficient platform for the transcorneal delivery of lipophilic drugs including most antifungals.
30077759	10	22	theme	CCMD	1484:1487	arg1	formula					1489:1495	The optimized CCMD formula	1470:1495	The optimized CCMD formula	1470:1495	The optimized CCMD formula was found to be significantly safe, stable, mucoadhesive and efficient to permeate the drug through rabbits' corneas.
30077759	10	22	theme	CCMD	1484:1487	arg1	safe					1527:1530	safe	1527:1530	safe	1527:1530	The optimized CCMD formula was found to be significantly safe, stable, mucoadhesive and efficient to permeate the drug through rabbits' corneas.
30077759	3	23	theme	study	441:445	arg1	aim					429:431	The aim	425:431	The aim of this study	425:445	The aim of this study was to enhance itraconazole corneal penetration through utilizing the minimum surfactant amount in presence of β-cyclodextrin which acted as a dissolution and permeation enhancer.
30077759	0	24	theme	insoluble	123:131	arg1	drug					133:136	a practically insoluble drug	109:136	a practically insoluble drug	109:136	Optimization of β-cyclodextrin consolidated micellar dispersion for promoting the transcorneal permeation of a practically insoluble drug.
30077759	6	25	theme	particle	911:918	arg1	size					920:923	particle size	911:923	particle size	911:923	The prepared CCMD were characterized through the analysis of their particle size, zeta potential and solubilization efficiency.
30077759	9	26	theme	safety	1424:1429	arg1	profiles					1444:1451	the ex vivo/in vivo permeation, safety and efficacy profiles	1392:1451	the ex vivo/in vivo permeation, safety and efficacy profiles	1392:1451	Moreover, the ex vivo/in vivo permeation, safety and efficacy profiles were determined.
30077759	1	27	theme	antifungal	196:205	arg1	drugs					207:211	antifungal drugs	196:211	antifungal drugs	196:211	Development of efficient ocular drug delivery system for antifungal drugs becomes a must nowadays to face and eradicate the widely spread ophthalmic fungal infections.
30077759	5	28	theme	dispersion	822:831	arg1	technique					833:841	a modified melt dispersion technique	806:841	a modified melt dispersion technique	806:841	The preparation was done according to a modified melt dispersion technique.
30077759	7	29	theme	w/w	1104:1106	arg1	ratios					1126:1131	30:1 w/w cyclodextrin/drug ratios	1099:1131	30:1 w/w cyclodextrin/drug ratios	1099:1131	The optimum formula was chosen based on a factorial response surface analysis and it was composed of 17:1 w/w surfactant/drug, 30:1 w/w cyclodextrin/drug ratios and 0.02% polyethylene oxide.
30077759	7	30	theme	optimum	976:982	arg1	formula					984:990	The optimum formula	972:990	The optimum formula	972:990	The optimum formula was chosen based on a factorial response surface analysis and it was composed of 17:1 w/w surfactant/drug, 30:1 w/w cyclodextrin/drug ratios and 0.02% polyethylene oxide.
30077759	4	31	theme	initial	706:712	arg1	screening					714:722	an initial screening	703:722	an initial screening to select the composition of surfactant(s)	703:765	β-Cyclodextrin consolidated micellar dispersions (CCMD) were prepared after an initial screening to select the composition of surfactant(s).
30077759	4	32	theme	surfactant	753:762	arg1	composition					738:748	the composition	734:748	the composition of surfactant(s)	734:765	β-Cyclodextrin consolidated micellar dispersions (CCMD) were prepared after an initial screening to select the composition of surfactant(s).
30077759	2	33	theme	antifungal	332:341	arg1	Itraconazole					307:318	Itraconazole	307:318	Itraconazole	307:318	Itraconazole, a triazole antifungal, is struggling to penetrate the cornea and subsequently, its efficacy is limited.
30077759	2	33	theme	antifungal	332:341	arg1	triazole					323:330	a triazole antifungal	321:341	a triazole antifungal	321:341	Itraconazole, a triazole antifungal, is struggling to penetrate the cornea and subsequently, its efficacy is limited.
30077759	9	34	theme	efficacy	1435:1442	arg1	profiles					1444:1451	the ex vivo/in vivo permeation, safety and efficacy profiles	1392:1451	the ex vivo/in vivo permeation, safety and efficacy profiles	1392:1451	Moreover, the ex vivo/in vivo permeation, safety and efficacy profiles were determined.
30077759	8	35	theme	inhibitory	1356:1365	arg1	concentration					1367:1379	the minimum inhibitory concentration	1344:1379	the minimum inhibitory concentration	1344:1379	This formula was subjected to in vitro characterization including release, imaging by transmission electron microscope, mucoadhesion, stability, in addition to the determination of the minimum inhibitory concentration.
30077759	3	36	theme	itraconazole	462:473	arg1	penetration					483:493	itraconazole corneal penetration	462:493	itraconazole corneal penetration	462:493	The aim of this study was to enhance itraconazole corneal penetration through utilizing the minimum surfactant amount in presence of β-cyclodextrin which acted as a dissolution and permeation enhancer.
30077759	3	37	theme	dissolution	590:600	arg1	enhancer					617:624	a dissolution and permeation enhancer	588:624	a dissolution and permeation enhancer	588:624	The aim of this study was to enhance itraconazole corneal penetration through utilizing the minimum surfactant amount in presence of β-cyclodextrin which acted as a dissolution and permeation enhancer.
30077759	3	37	theme	dissolution	590:600	arg1	β-cyclodextrin					558:571	β-cyclodextrin	558:571	β-cyclodextrin which acted as a dissolution and permeation enhancer	558:624	The aim of this study was to enhance itraconazole corneal penetration through utilizing the minimum surfactant amount in presence of β-cyclodextrin which acted as a dissolution and permeation enhancer.
30077759	7	38	theme	30:1	1099:1102	arg1	ratios					1126:1131	30:1 w/w cyclodextrin/drug ratios	1099:1131	30:1 w/w cyclodextrin/drug ratios	1099:1131	The optimum formula was chosen based on a factorial response surface analysis and it was composed of 17:1 w/w surfactant/drug, 30:1 w/w cyclodextrin/drug ratios and 0.02% polyethylene oxide.
30077759	11	39	theme	transcorneal	1716:1727	arg1	delivery					1729:1736	the transcorneal delivery	1712:1736	the transcorneal delivery of lipophilic drugs including most antifungals	1712:1783	Consequently, the optimized CCMD formulation can be a promising, safe and efficient platform for the transcorneal delivery of lipophilic drugs including most antifungals.
30077759	3	40	theme	surfactant	525:534	arg1	amount					536:541	the minimum surfactant amount	513:541	the minimum surfactant amount in presence of β-cyclodextrin which acted as a dissolution and permeation enhancer	513:624	The aim of this study was to enhance itraconazole corneal penetration through utilizing the minimum surfactant amount in presence of β-cyclodextrin which acted as a dissolution and permeation enhancer.
30077759	3	41	theme	corneal	475:481	arg1	penetration					483:493	itraconazole corneal penetration	462:493	itraconazole corneal penetration	462:493	The aim of this study was to enhance itraconazole corneal penetration through utilizing the minimum surfactant amount in presence of β-cyclodextrin which acted as a dissolution and permeation enhancer.
30077759	7	42	theme	w/w	1078:1080	arg1	surfactant/drug					1082:1096	17:1 w/w surfactant/drug	1073:1096	17:1 w/w surfactant/drug	1073:1096	The optimum formula was chosen based on a factorial response surface analysis and it was composed of 17:1 w/w surfactant/drug, 30:1 w/w cyclodextrin/drug ratios and 0.02% polyethylene oxide.
30077759	0	43	theme	micellar	44:51	arg1	dispersion					53:62	micellar dispersion	44:62	micellar dispersion	44:62	Optimization of β-cyclodextrin consolidated micellar dispersion for promoting the transcorneal permeation of a practically insoluble drug.
30077759	9	44	theme	ex	1396:1397	arg1	profiles					1444:1451	the ex vivo/in vivo permeation, safety and efficacy profiles	1392:1451	the ex vivo/in vivo permeation, safety and efficacy profiles	1392:1451	Moreover, the ex vivo/in vivo permeation, safety and efficacy profiles were determined.
30077759	9	45	theme	permeation	1412:1421	arg1	profiles					1444:1451	the ex vivo/in vivo permeation, safety and efficacy profiles	1392:1451	the ex vivo/in vivo permeation, safety and efficacy profiles	1392:1451	Moreover, the ex vivo/in vivo permeation, safety and efficacy profiles were determined.
30077759	8	46	theme	in	1193:1194	arg1	characterization					1202:1217	in vitro characterization	1193:1217	in vitro characterization including release, imaging by transmission electron microscope, mucoadhesion, stability, in addition to the determination of the minimum inhibitory concentration	1193:1379	This formula was subjected to in vitro characterization including release, imaging by transmission electron microscope, mucoadhesion, stability, in addition to the determination of the minimum inhibitory concentration.
30077759	5	47	theme	modified	808:815	arg1	technique					833:841	a modified melt dispersion technique	806:841	a modified melt dispersion technique	806:841	The preparation was done according to a modified melt dispersion technique.
30077759	9	48	dep	ex	1396:1397	arg1	vivo					1407:1410	vivo	1407:1410	vivo	1407:1410	Moreover, the ex vivo/in vivo permeation, safety and efficacy profiles were determined.
30077759	5	49	theme	melt	817:820	arg1	technique					833:841	a modified melt dispersion technique	806:841	a modified melt dispersion technique	806:841	The preparation was done according to a modified melt dispersion technique.
30077759	7	50	theme	17:1	1073:1076	arg1	surfactant/drug					1082:1096	17:1 w/w surfactant/drug	1073:1096	17:1 w/w surfactant/drug	1073:1096	The optimum formula was chosen based on a factorial response surface analysis and it was composed of 17:1 w/w surfactant/drug, 30:1 w/w cyclodextrin/drug ratios and 0.02% polyethylene oxide.
30077759	11	51	theme	drugs	1752:1756	arg1	delivery					1729:1736	the transcorneal delivery	1712:1736	the transcorneal delivery of lipophilic drugs including most antifungals	1712:1783	Consequently, the optimized CCMD formulation can be a promising, safe and efficient platform for the transcorneal delivery of lipophilic drugs including most antifungals.
30077759	3	52	theme	permeation	606:615	arg1	enhancer					617:624	a dissolution and permeation enhancer	588:624	a dissolution and permeation enhancer	588:624	The aim of this study was to enhance itraconazole corneal penetration through utilizing the minimum surfactant amount in presence of β-cyclodextrin which acted as a dissolution and permeation enhancer.
30077759	3	52	theme	permeation	606:615	arg1	β-cyclodextrin					558:571	β-cyclodextrin	558:571	β-cyclodextrin which acted as a dissolution and permeation enhancer	558:624	The aim of this study was to enhance itraconazole corneal penetration through utilizing the minimum surfactant amount in presence of β-cyclodextrin which acted as a dissolution and permeation enhancer.
30077759	7	53	theme	surface	1033:1039	arg1	analysis					1041:1048	a factorial response surface analysis	1012:1048	a factorial response surface analysis	1012:1048	The optimum formula was chosen based on a factorial response surface analysis and it was composed of 17:1 w/w surfactant/drug, 30:1 w/w cyclodextrin/drug ratios and 0.02% polyethylene oxide.
30077759	10	54	theme	optimized	1474:1482	arg1	formula					1489:1495	The optimized CCMD formula	1470:1495	The optimized CCMD formula	1470:1495	The optimized CCMD formula was found to be significantly safe, stable, mucoadhesive and efficient to permeate the drug through rabbits' corneas.
30077759	10	54	theme	optimized	1474:1482	arg1	safe					1527:1530	safe	1527:1530	safe	1527:1530	The optimized CCMD formula was found to be significantly safe, stable, mucoadhesive and efficient to permeate the drug through rabbits' corneas.
30077759	6	55	theme	efficiency	960:969	arg1	analysis					893:900	the analysis	889:900	the analysis of their particle size, zeta potential and solubilization efficiency	889:969	The prepared CCMD were characterized through the analysis of their particle size, zeta potential and solubilization efficiency.
30077759	11	56	theme	lipophilic	1741:1750	arg1	drugs					1752:1756	lipophilic drugs	1741:1756	lipophilic drugs including most antifungals	1741:1783	Consequently, the optimized CCMD formulation can be a promising, safe and efficient platform for the transcorneal delivery of lipophilic drugs including most antifungals.
30077759	11	56	theme	lipophilic	1741:1750	arg1	antifungals					1773:1783	most antifungals	1768:1783	most antifungals	1768:1783	Consequently, the optimized CCMD formulation can be a promising, safe and efficient platform for the transcorneal delivery of lipophilic drugs including most antifungals.
30077759	4	57	theme	consolidated	642:653	arg1	CCMD					677:680	CCMD	677:680	CCMD	677:680	β-Cyclodextrin consolidated micellar dispersions (CCMD) were prepared after an initial screening to select the composition of surfactant(s).
30077759	4	57	theme	consolidated	642:653	arg1	dispersions					664:674	β-Cyclodextrin consolidated micellar dispersions	627:674	β-Cyclodextrin consolidated micellar dispersions (CCMD)	627:681	β-Cyclodextrin consolidated micellar dispersions (CCMD) were prepared after an initial screening to select the composition of surfactant(s).
30077759	8	58	theme	minimum	1348:1354	arg1	concentration					1367:1379	the minimum inhibitory concentration	1344:1379	the minimum inhibitory concentration	1344:1379	This formula was subjected to in vitro characterization including release, imaging by transmission electron microscope, mucoadhesion, stability, in addition to the determination of the minimum inhibitory concentration.
30077759	7	59	theme	factorial	1014:1022	arg1	analysis					1041:1048	a factorial response surface analysis	1012:1048	a factorial response surface analysis	1012:1048	The optimum formula was chosen based on a factorial response surface analysis and it was composed of 17:1 w/w surfactant/drug, 30:1 w/w cyclodextrin/drug ratios and 0.02% polyethylene oxide.
30077759	1	60	theme	efficient	154:162	arg1	system					185:190	efficient ocular drug delivery system	154:190	efficient ocular drug delivery system for antifungal drugs	154:211	Development of efficient ocular drug delivery system for antifungal drugs becomes a must nowadays to face and eradicate the widely spread ophthalmic fungal infections.
30077759	8	61	theme	concentration	1367:1379	arg1	determination					1327:1339	the determination	1323:1339	the determination of the minimum inhibitory concentration	1323:1379	This formula was subjected to in vitro characterization including release, imaging by transmission electron microscope, mucoadhesion, stability, in addition to the determination of the minimum inhibitory concentration.
30077759	4	62	theme	β-Cyclodextrin	627:640	arg1	CCMD					677:680	CCMD	677:680	CCMD	677:680	β-Cyclodextrin consolidated micellar dispersions (CCMD) were prepared after an initial screening to select the composition of surfactant(s).
30077759	4	62	theme	β-Cyclodextrin	627:640	arg1	dispersions					664:674	β-Cyclodextrin consolidated micellar dispersions	627:674	β-Cyclodextrin consolidated micellar dispersions (CCMD)	627:681	β-Cyclodextrin consolidated micellar dispersions (CCMD) were prepared after an initial screening to select the composition of surfactant(s).
30077759	0	63	theme	transcorneal	82:93	arg1	permeation					95:104	the transcorneal permeation	78:104	the transcorneal permeation of a practically insoluble drug	78:136	Optimization of β-cyclodextrin consolidated micellar dispersion for promoting the transcorneal permeation of a practically insoluble drug.
30077759	6	64	theme	prepared	848:855	arg1	CCMD					857:860	The prepared CCMD	844:860	The prepared CCMD	844:860	The prepared CCMD were characterized through the analysis of their particle size, zeta potential and solubilization efficiency.
30077759	1	65	dep	a	221:221	arg1	must					223:226	must	223:226	must	223:226	Development of efficient ocular drug delivery system for antifungal drugs becomes a must nowadays to face and eradicate the widely spread ophthalmic fungal infections.
30077759	7	66	theme	response	1024:1031	arg1	analysis					1041:1048	a factorial response surface analysis	1012:1048	a factorial response surface analysis	1012:1048	The optimum formula was chosen based on a factorial response surface analysis and it was composed of 17:1 w/w surfactant/drug, 30:1 w/w cyclodextrin/drug ratios and 0.02% polyethylene oxide.
30077759	1	67	theme	ocular	164:169	arg1	system					185:190	efficient ocular drug delivery system	154:190	efficient ocular drug delivery system for antifungal drugs	154:211	Development of efficient ocular drug delivery system for antifungal drugs becomes a must nowadays to face and eradicate the widely spread ophthalmic fungal infections.
30077759	11	68	theme	most	1768:1771	arg1	antifungals					1773:1783	most antifungals	1768:1783	most antifungals	1768:1783	Consequently, the optimized CCMD formulation can be a promising, safe and efficient platform for the transcorneal delivery of lipophilic drugs including most antifungals.
30077759	8	69	theme	electron	1262:1269	arg1	mucoadhesion					1283:1294	mucoadhesion	1283:1294	mucoadhesion	1283:1294	This formula was subjected to in vitro characterization including release, imaging by transmission electron microscope, mucoadhesion, stability, in addition to the determination of the minimum inhibitory concentration.
30077759	8	69	theme	electron	1262:1269	arg1	microscope					1271:1280	transmission electron microscope	1249:1280	transmission electron microscope	1249:1280	This formula was subjected to in vitro characterization including release, imaging by transmission electron microscope, mucoadhesion, stability, in addition to the determination of the minimum inhibitory concentration.
30077759	8	69	theme	electron	1262:1269	arg1	stability					1297:1305	stability	1297:1305	stability	1297:1305	This formula was subjected to in vitro characterization including release, imaging by transmission electron microscope, mucoadhesion, stability, in addition to the determination of the minimum inhibitory concentration.
30077759	6	70	theme	solubilization	945:958	arg1	efficiency					960:969	solubilization efficiency	945:969	solubilization efficiency	945:969	The prepared CCMD were characterized through the analysis of their particle size, zeta potential and solubilization efficiency.
30077759	0	71	theme	β-cyclodextrin	16:29	arg1	Optimization					0:11	Optimization	0:11	Optimization of β-cyclodextrin	0:29	Optimization of β-cyclodextrin consolidated micellar dispersion for promoting the transcorneal permeation of a practically insoluble drug.
30077759	11	72	theme	optimized	1633:1641	arg1	formulation					1648:1658	the optimized CCMD formulation	1629:1658	the optimized CCMD formulation	1629:1658	Consequently, the optimized CCMD formulation can be a promising, safe and efficient platform for the transcorneal delivery of lipophilic drugs including most antifungals.
30077759	11	72	theme	optimized	1633:1641	arg1	platform					1699:1706	a promising, safe and efficient platform	1667:1706	a promising, safe and efficient platform for the transcorneal delivery of lipophilic drugs including most antifungals	1667:1783	Consequently, the optimized CCMD formulation can be a promising, safe and efficient platform for the transcorneal delivery of lipophilic drugs including most antifungals.
30077759	1	73	theme	drug	171:174	arg1	system					185:190	efficient ocular drug delivery system	154:190	efficient ocular drug delivery system for antifungal drugs	154:211	Development of efficient ocular drug delivery system for antifungal drugs becomes a must nowadays to face and eradicate the widely spread ophthalmic fungal infections.
30077759	4	74	theme	micellar	655:662	arg1	CCMD					677:680	CCMD	677:680	CCMD	677:680	β-Cyclodextrin consolidated micellar dispersions (CCMD) were prepared after an initial screening to select the composition of surfactant(s).
30077759	4	74	theme	micellar	655:662	arg1	dispersions					664:674	β-Cyclodextrin consolidated micellar dispersions	627:674	β-Cyclodextrin consolidated micellar dispersions (CCMD)	627:681	β-Cyclodextrin consolidated micellar dispersions (CCMD) were prepared after an initial screening to select the composition of surfactant(s).
29757056	2	0	theme	hydrolyzed	645:654	arg1	COLLAGEN					672:679	a hydrolyzed collagen blend (COLLAGEN) or whey (WHEY) protein 45 min	643:710	COLLAGEN	672:679	In a repeated-measures design (after adhering to a 36-hr exercise-dietary intervention to standardize preexercise muscle glycogen), eight males completed a 75-min nonexhaustive cycling protocol and consumed 22 g of a hydrolyzed collagen blend (COLLAGEN) or whey (WHEY) protein 45 min prior to exercise, 22 g during exercise, and 22 g immediately postexercise.
29757056	9	1	theme	leucine	1903:1909	arg1	availability					1911:1922	increased leucine availability	1893:1922	increased leucine availability	1893:1922	By contrast, whey protein augments postexercise p70S6K activity compared with hydrolyzed collagen, as likely mediated via increased leucine availability.
29757056	1	2	theme	signaling	256:264	arg1	responses					266:274	skeletal muscle cell signaling responses	235:274	skeletal muscle cell signaling responses associated with mitochondrial biogenesis and protein synthesis in recovery from an acute training session completed with low carbohydrate availability	235:425	We examined the effects of whey versus collagen protein on skeletal muscle cell signaling responses associated with mitochondrial biogenesis and protein synthesis in recovery from an acute training session completed with low carbohydrate availability.
29757056	7	3	theme	1.5 hr	1425:1430	arg1	p < .05					1446:1452	p < .05	1446:1452	p < .05	1446:1452	Postexercise feeding increased p70S6K1 activity at 1.5 hr postexercise (p < .05), the magnitude of which was greater (p < .05) in WHEY (180 ± 105 fmol·min-1·mg-1) versus COLLAGEN (73 ± 42 fmol·min-1·mg-1).
29757056	7	3	theme	1.5 hr	1425:1430	arg1	postexercise					1432:1443	1.5 hr postexercise	1425:1443	1.5 hr postexercise (p < .05)	1425:1453	Postexercise feeding increased p70S6K1 activity at 1.5 hr postexercise (p < .05), the magnitude of which was greater (p < .05) in WHEY (180 ± 105 fmol·min-1·mg-1) versus COLLAGEN (73 ± 42 fmol·min-1·mg-1).
29757056	1	4	from	synthesis	329:337	arg1	session					374:380	an acute training session	356:380	an acute training session completed with low carbohydrate availability	356:425	We examined the effects of whey versus collagen protein on skeletal muscle cell signaling responses associated with mitochondrial biogenesis and protein synthesis in recovery from an acute training session completed with low carbohydrate availability.
29757056	1	4	from	synthesis	329:337	arg1	recovery					342:349	recovery	342:349	recovery from an acute training session completed with low carbohydrate availability	342:425	We examined the effects of whey versus collagen protein on skeletal muscle cell signaling responses associated with mitochondrial biogenesis and protein synthesis in recovery from an acute training session completed with low carbohydrate availability.
29757056	8	5	theme	training	1695:1702	arg1	session					1704:1710	a training session	1693:1710	a training session deliberately completed with low carbohydrate availability	1693:1768	We conclude that protein composition does not modulate markers of mitochondrial biogenesis when in recovery from a training session deliberately completed with low carbohydrate availability.
29757056	4	6	from	increases	963:971	arg1	p = .02					1033:1039	p = .02	1033:1039	p = .02	1033:1039	WHEY protein induced greater increases in plasma branched chain amino acids (p = .03) and leucine (p = .02) than COLLAGEN.
29757056	4	6	from	increases	963:971	arg1	p = .03					1011:1017	p = .03	1011:1017	p = .03	1011:1017	WHEY protein induced greater increases in plasma branched chain amino acids (p = .03) and leucine (p = .02) than COLLAGEN.
29757056	4	6	from	increases	963:971	arg1	leucine					1024:1030	leucine	1024:1030	leucine (p = .02)	1024:1040	WHEY protein induced greater increases in plasma branched chain amino acids (p = .03) and leucine (p = .02) than COLLAGEN.
29757056	4	6	from	increases	963:971	arg1	acids					1004:1008	plasma branched chain amino acids	976:1008	plasma branched chain amino acids (p = .03)	976:1018	WHEY protein induced greater increases in plasma branched chain amino acids (p = .03) and leucine (p = .02) than COLLAGEN.
29757056	2	7	theme	COLLAGEN	672:679	arg1	22 g					635:638	22 g	635:638	22 g of a hydrolyzed collagen blend (COLLAGEN) or whey (WHEY) protein 45 min prior to exercise, 22 g during exercise, and 22 g immediately postexercise	635:785	In a repeated-measures design (after adhering to a 36-hr exercise-dietary intervention to standardize preexercise muscle glycogen), eight males completed a 75-min nonexhaustive cycling protocol and consumed 22 g of a hydrolyzed collagen blend (COLLAGEN) or whey (WHEY) protein 45 min prior to exercise, 22 g during exercise, and 22 g immediately postexercise.
29757056	6	8	dep	suppressed	1269:1278	arg1	≈25 fmol·min-1·mg-1					1352:1370	≈25 fmol·min-1·mg-1	1352:1370	≈25 fmol·min-1·mg-1	1352:1370	Exercise suppressed (p < .05) p70S6K1 activity in both conditions immediately postexercise (≈25 fmol·min-1·mg-1).
29757056	2	9	theme	blend	665:669	arg1	COLLAGEN					672:679	a hydrolyzed collagen blend (COLLAGEN) or whey (WHEY) protein 45 min	643:710	COLLAGEN	672:679	In a repeated-measures design (after adhering to a 36-hr exercise-dietary intervention to standardize preexercise muscle glycogen), eight males completed a 75-min nonexhaustive cycling protocol and consumed 22 g of a hydrolyzed collagen blend (COLLAGEN) or whey (WHEY) protein 45 min prior to exercise, 22 g during exercise, and 22 g immediately postexercise.
29757056	2	10	theme	nonexhaustive	591:603	arg1	protocol					613:620	a 75-min nonexhaustive cycling protocol	582:620	a 75-min nonexhaustive cycling protocol	582:620	In a repeated-measures design (after adhering to a 36-hr exercise-dietary intervention to standardize preexercise muscle glycogen), eight males completed a 75-min nonexhaustive cycling protocol and consumed 22 g of a hydrolyzed collagen blend (COLLAGEN) or whey (WHEY) protein 45 min prior to exercise, 22 g during exercise, and 22 g immediately postexercise.
29757056	7	11	theme	Postexercise	1374:1385	arg1	feeding					1387:1393	Postexercise feeding	1374:1393	Postexercise feeding	1374:1393	Postexercise feeding increased p70S6K1 activity at 1.5 hr postexercise (p < .05), the magnitude of which was greater (p < .05) in WHEY (180 ± 105 fmol·min-1·mg-1) versus COLLAGEN (73 ± 42 fmol·min-1·mg-1).
29757056	1	12	theme	carbohydrate	401:412	arg1	availability					414:425	low carbohydrate availability	397:425	low carbohydrate availability	397:425	We examined the effects of whey versus collagen protein on skeletal muscle cell signaling responses associated with mitochondrial biogenesis and protein synthesis in recovery from an acute training session completed with low carbohydrate availability.
29757056	8	13	theme	carbohydrate	1744:1755	arg1	availability					1757:1768	low carbohydrate availability	1740:1768	low carbohydrate availability	1740:1768	We conclude that protein composition does not modulate markers of mitochondrial biogenesis when in recovery from a training session deliberately completed with low carbohydrate availability.
29757056	1	14	theme	whey	203:206	arg1	protein					224:230	whey versus collagen protein	203:230	protein	224:230	We examined the effects of whey versus collagen protein on skeletal muscle cell signaling responses associated with mitochondrial biogenesis and protein synthesis in recovery from an acute training session completed with low carbohydrate availability.
29757056	4	15	theme	greater	955:961	arg1	increases					963:971	greater increases	955:971	greater increases in plasma branched chain amino acids (p = .03) and leucine (p = .02)	955:1040	WHEY protein induced greater increases in plasma branched chain amino acids (p = .03) and leucine (p = .02) than COLLAGEN.
29757056	7	16	dep	greater	1483:1489	arg1	p < .05					1492:1498	p < .05	1492:1498	p < .05	1492:1498	Postexercise feeding increased p70S6K1 activity at 1.5 hr postexercise (p < .05), the magnitude of which was greater (p < .05) in WHEY (180 ± 105 fmol·min-1·mg-1) versus COLLAGEN (73 ± 42 fmol·min-1·mg-1).
29757056	5	17	from	increases	1092:1100	arg1	mRNA					1123:1126	PGC-1α (fivefold) mRNA	1105:1126	PGC-1α (fivefold) mRNA	1105:1126	Exercise induced (p < .05) similar increases in PGC-1α (fivefold) mRNA at 1.5 hr postexercise between conditions, although no effect of exercise (p > .05) was observed for p53, Parkin, and Beclin1 mRNA.
29757056	1	18	theme	collagen	215:222	arg1	protein					224:230	whey versus collagen protein	203:230	protein	224:230	We examined the effects of whey versus collagen protein on skeletal muscle cell signaling responses associated with mitochondrial biogenesis and protein synthesis in recovery from an acute training session completed with low carbohydrate availability.
29757056	0	19	theme	Low	145:147	arg1	Availability					162:173	Low Carbohydrate Availability	145:173	Low Carbohydrate Availability	145:173	Whey Protein Augments Leucinemia and Postexercise p70S6K1 Activity Compared With a Hydrolyzed Collagen Blend When in Recovery From Training With Low Carbohydrate Availability.
29757056	5	20	theme	Beclin1	1246:1252	arg1	mRNA					1254:1257	Beclin1 mRNA	1246:1257	Beclin1 mRNA	1246:1257	Exercise induced (p < .05) similar increases in PGC-1α (fivefold) mRNA at 1.5 hr postexercise between conditions, although no effect of exercise (p > .05) was observed for p53, Parkin, and Beclin1 mRNA.
29757056	3	21	theme	muscle	817:822	arg1	content					833:839	muscle glycogen content	817:839	muscle glycogen content	817:839	Exercise decreased (p < .05) muscle glycogen content by comparable levels from pre- to postexercise in both trials (≈300-150 mmol/kg·dry weight).
29757056	4	22	theme	amino	998:1002	arg1	p = .03					1011:1017	p = .03	1011:1017	p = .03	1011:1017	WHEY protein induced greater increases in plasma branched chain amino acids (p = .03) and leucine (p = .02) than COLLAGEN.
29757056	4	22	theme	amino	998:1002	arg1	acids					1004:1008	plasma branched chain amino acids	976:1008	plasma branched chain amino acids (p = .03)	976:1018	WHEY protein induced greater increases in plasma branched chain amino acids (p = .03) and leucine (p = .02) than COLLAGEN.
29757056	0	23	with	Recovery	117:124	arg1	Availability					162:173	Low Carbohydrate Availability	145:173	Low Carbohydrate Availability	145:173	Whey Protein Augments Leucinemia and Postexercise p70S6K1 Activity Compared With a Hydrolyzed Collagen Blend When in Recovery From Training With Low Carbohydrate Availability.
29757056	5	24	theme	fivefold	1113:1120	arg1	mRNA					1123:1126	PGC-1α (fivefold) mRNA	1105:1126	PGC-1α (fivefold) mRNA	1105:1126	Exercise induced (p < .05) similar increases in PGC-1α (fivefold) mRNA at 1.5 hr postexercise between conditions, although no effect of exercise (p > .05) was observed for p53, Parkin, and Beclin1 mRNA.
29757056	2	25	theme	preexercise	530:540	arg1	glycogen					549:556	preexercise muscle glycogen	530:556	preexercise muscle glycogen	530:556	In a repeated-measures design (after adhering to a 36-hr exercise-dietary intervention to standardize preexercise muscle glycogen), eight males completed a 75-min nonexhaustive cycling protocol and consumed 22 g of a hydrolyzed collagen blend (COLLAGEN) or whey (WHEY) protein 45 min prior to exercise, 22 g during exercise, and 22 g immediately postexercise.
29757056	3	26	theme	comparable	844:853	arg1	levels					855:860	comparable levels	844:860	comparable levels from pre- to postexercise in both trials (≈300-150 mmol/kg·dry weight)	844:931	Exercise decreased (p < .05) muscle glycogen content by comparable levels from pre- to postexercise in both trials (≈300-150 mmol/kg·dry weight).
29757056	1	27	theme	acute	359:363	arg1	session					374:380	an acute training session	356:380	an acute training session completed with low carbohydrate availability	356:425	We examined the effects of whey versus collagen protein on skeletal muscle cell signaling responses associated with mitochondrial biogenesis and protein synthesis in recovery from an acute training session completed with low carbohydrate availability.
29757056	2	28	theme	whey	685:688	arg1	45 min					705:710	a hydrolyzed collagen blend (COLLAGEN) or whey (WHEY) protein 45 min	643:710	45 min	705:710	In a repeated-measures design (after adhering to a 36-hr exercise-dietary intervention to standardize preexercise muscle glycogen), eight males completed a 75-min nonexhaustive cycling protocol and consumed 22 g of a hydrolyzed collagen blend (COLLAGEN) or whey (WHEY) protein 45 min prior to exercise, 22 g during exercise, and 22 g immediately postexercise.
29757056	0	29	theme	Collagen	94:101	arg1	Blend					103:107	a Hydrolyzed Collagen Blend	81:107	a Hydrolyzed Collagen Blend When in Recovery From Training With Low Carbohydrate Availability	81:173	Whey Protein Augments Leucinemia and Postexercise p70S6K1 Activity Compared With a Hydrolyzed Collagen Blend When in Recovery From Training With Low Carbohydrate Availability.
29757056	2	30	dep	intervention	502:513	arg1	standardize					518:528	standardize	518:528	to standardize preexercise muscle glycogen	515:556	In a repeated-measures design (after adhering to a 36-hr exercise-dietary intervention to standardize preexercise muscle glycogen), eight males completed a 75-min nonexhaustive cycling protocol and consumed 22 g of a hydrolyzed collagen blend (COLLAGEN) or whey (WHEY) protein 45 min prior to exercise, 22 g during exercise, and 22 g immediately postexercise.
29757056	4	31	theme	branched	983:990	arg1	p = .03					1011:1017	p = .03	1011:1017	p = .03	1011:1017	WHEY protein induced greater increases in plasma branched chain amino acids (p = .03) and leucine (p = .02) than COLLAGEN.
29757056	4	31	theme	branched	983:990	arg1	acids					1004:1008	plasma branched chain amino acids	976:1008	plasma branched chain amino acids (p = .03)	976:1018	WHEY protein induced greater increases in plasma branched chain amino acids (p = .03) and leucine (p = .02) than COLLAGEN.
29757056	3	32	dep	decreased	797:805	arg1	p < .05					808:814	p < .05	808:814	p < .05	808:814	Exercise decreased (p < .05) muscle glycogen content by comparable levels from pre- to postexercise in both trials (≈300-150 mmol/kg·dry weight).
29757056	3	33	theme	≈300-150 mmol/kg·dry	904:923	arg1	trials					896:901	both trials	891:901	both trials (≈300-150 mmol/kg·dry weight)	891:931	Exercise decreased (p < .05) muscle glycogen content by comparable levels from pre- to postexercise in both trials (≈300-150 mmol/kg·dry weight).
29757056	3	33	theme	≈300-150 mmol/kg·dry	904:923	arg1	weight					925:930	≈300-150 mmol/kg·dry weight	904:930	≈300-150 mmol/kg·dry weight	904:930	Exercise decreased (p < .05) muscle glycogen content by comparable levels from pre- to postexercise in both trials (≈300-150 mmol/kg·dry weight).
29757056	9	34	theme	postexercise	1806:1817	arg1	activity					1826:1833	postexercise p70S6K activity	1806:1833	postexercise p70S6K activity	1806:1833	By contrast, whey protein augments postexercise p70S6K activity compared with hydrolyzed collagen, as likely mediated via increased leucine availability.
29757056	2	35	theme	protein	697:703	arg1	45 min					705:710	a hydrolyzed collagen blend (COLLAGEN) or whey (WHEY) protein 45 min	643:710	45 min	705:710	In a repeated-measures design (after adhering to a 36-hr exercise-dietary intervention to standardize preexercise muscle glycogen), eight males completed a 75-min nonexhaustive cycling protocol and consumed 22 g of a hydrolyzed collagen blend (COLLAGEN) or whey (WHEY) protein 45 min prior to exercise, 22 g during exercise, and 22 g immediately postexercise.
29757056	0	36	theme	Whey	0:3	arg1	Protein					5:11	Whey Protein	0:11	Whey Protein	0:11	Whey Protein Augments Leucinemia and Postexercise p70S6K1 Activity Compared With a Hydrolyzed Collagen Blend When in Recovery From Training With Low Carbohydrate Availability.
29757056	2	37	theme	WHEY	691:694	arg1	45 min					705:710	a hydrolyzed collagen blend (COLLAGEN) or whey (WHEY) protein 45 min	643:710	45 min	705:710	In a repeated-measures design (after adhering to a 36-hr exercise-dietary intervention to standardize preexercise muscle glycogen), eight males completed a 75-min nonexhaustive cycling protocol and consumed 22 g of a hydrolyzed collagen blend (COLLAGEN) or whey (WHEY) protein 45 min prior to exercise, 22 g during exercise, and 22 g immediately postexercise.
29757056	7	38	theme	WHEY	1504:1507	arg1	180 ± 105 fmol·min-1·mg-1					1510:1534	WHEY (180 ± 105 fmol·min-1·mg-1)	1504:1535	WHEY (180 ± 105 fmol·min-1·mg-1)	1504:1535	Postexercise feeding increased p70S6K1 activity at 1.5 hr postexercise (p < .05), the magnitude of which was greater (p < .05) in WHEY (180 ± 105 fmol·min-1·mg-1) versus COLLAGEN (73 ± 42 fmol·min-1·mg-1).
29757056	2	39	theme	repeated-measures	433:449	arg1	design					451:456	a repeated-measures design	431:456	a repeated-measures design (after adhering to a 36-hr exercise-dietary intervention to standardize preexercise muscle glycogen)	431:557	In a repeated-measures design (after adhering to a 36-hr exercise-dietary intervention to standardize preexercise muscle glycogen), eight males completed a 75-min nonexhaustive cycling protocol and consumed 22 g of a hydrolyzed collagen blend (COLLAGEN) or whey (WHEY) protein 45 min prior to exercise, 22 g during exercise, and 22 g immediately postexercise.
29757056	1	40	theme	protein	321:327	arg1	synthesis					329:337	protein synthesis	321:337	protein synthesis	321:337	We examined the effects of whey versus collagen protein on skeletal muscle cell signaling responses associated with mitochondrial biogenesis and protein synthesis in recovery from an acute training session completed with low carbohydrate availability.
29757056	2	41	theme	exercise-dietary	485:500	arg1	intervention					502:513	a 36-hr exercise-dietary intervention	477:513	a 36-hr exercise-dietary intervention to standardize preexercise muscle glycogen	477:556	In a repeated-measures design (after adhering to a 36-hr exercise-dietary intervention to standardize preexercise muscle glycogen), eight males completed a 75-min nonexhaustive cycling protocol and consumed 22 g of a hydrolyzed collagen blend (COLLAGEN) or whey (WHEY) protein 45 min prior to exercise, 22 g during exercise, and 22 g immediately postexercise.
29757056	5	42	theme	1.5 hr	1131:1136	arg1	postexercise					1138:1149	1.5 hr postexercise	1131:1149	1.5 hr postexercise between conditions	1131:1168	Exercise induced (p < .05) similar increases in PGC-1α (fivefold) mRNA at 1.5 hr postexercise between conditions, although no effect of exercise (p > .05) was observed for p53, Parkin, and Beclin1 mRNA.
29757056	2	43	theme	muscle	542:547	arg1	glycogen					549:556	preexercise muscle glycogen	530:556	preexercise muscle glycogen	530:556	In a repeated-measures design (after adhering to a 36-hr exercise-dietary intervention to standardize preexercise muscle glycogen), eight males completed a 75-min nonexhaustive cycling protocol and consumed 22 g of a hydrolyzed collagen blend (COLLAGEN) or whey (WHEY) protein 45 min prior to exercise, 22 g during exercise, and 22 g immediately postexercise.
29757056	0	44	theme	p70S6K1	50:56	arg1	Activity					58:65	Postexercise p70S6K1 Activity	37:65	Postexercise p70S6K1 Activity	37:65	Whey Protein Augments Leucinemia and Postexercise p70S6K1 Activity Compared With a Hydrolyzed Collagen Blend When in Recovery From Training With Low Carbohydrate Availability.
29757056	1	45	theme	protein	224:230	arg1	effects					192:198	the effects	188:198	the effects of whey versus collagen protein on skeletal muscle cell signaling responses associated with mitochondrial biogenesis and protein synthesis in recovery from an acute training session completed with low carbohydrate availability	188:425	We examined the effects of whey versus collagen protein on skeletal muscle cell signaling responses associated with mitochondrial biogenesis and protein synthesis in recovery from an acute training session completed with low carbohydrate availability.
29757056	0	46	from	Training	131:138	arg1	Recovery					117:124	Recovery	117:124	Recovery From Training With Low Carbohydrate Availability	117:173	Whey Protein Augments Leucinemia and Postexercise p70S6K1 Activity Compared With a Hydrolyzed Collagen Blend When in Recovery From Training With Low Carbohydrate Availability.
29757056	8	47	theme	mitochondrial	1646:1658	arg1	biogenesis					1660:1669	mitochondrial biogenesis	1646:1669	mitochondrial biogenesis	1646:1669	We conclude that protein composition does not modulate markers of mitochondrial biogenesis when in recovery from a training session deliberately completed with low carbohydrate availability.
29757056	1	48	theme	skeletal	235:242	arg1	responses					266:274	skeletal muscle cell signaling responses	235:274	skeletal muscle cell signaling responses associated with mitochondrial biogenesis and protein synthesis in recovery from an acute training session completed with low carbohydrate availability	235:425	We examined the effects of whey versus collagen protein on skeletal muscle cell signaling responses associated with mitochondrial biogenesis and protein synthesis in recovery from an acute training session completed with low carbohydrate availability.
29757056	9	49	theme	increased	1893:1901	arg1	availability					1911:1922	increased leucine availability	1893:1922	increased leucine availability	1893:1922	By contrast, whey protein augments postexercise p70S6K activity compared with hydrolyzed collagen, as likely mediated via increased leucine availability.
29757056	1	50	theme	cell	251:254	arg1	responses					266:274	skeletal muscle cell signaling responses	235:274	skeletal muscle cell signaling responses associated with mitochondrial biogenesis and protein synthesis in recovery from an acute training session completed with low carbohydrate availability	235:425	We examined the effects of whey versus collagen protein on skeletal muscle cell signaling responses associated with mitochondrial biogenesis and protein synthesis in recovery from an acute training session completed with low carbohydrate availability.
29757056	7	51	theme	postexercise	1432:1443	arg1	magnitude					1460:1468	the magnitude	1456:1468	the magnitude	1456:1468	Postexercise feeding increased p70S6K1 activity at 1.5 hr postexercise (p < .05), the magnitude of which was greater (p < .05) in WHEY (180 ± 105 fmol·min-1·mg-1) versus COLLAGEN (73 ± 42 fmol·min-1·mg-1).
29757056	7	51	theme	postexercise	1432:1443	arg1	greater					1483:1489	greater	1483:1489	greater	1483:1489	Postexercise feeding increased p70S6K1 activity at 1.5 hr postexercise (p < .05), the magnitude of which was greater (p < .05) in WHEY (180 ± 105 fmol·min-1·mg-1) versus COLLAGEN (73 ± 42 fmol·min-1·mg-1).
29757056	2	52	theme	postexercise	774:785	arg1	exercise					721:728	exercise	721:728	exercise	721:728	In a repeated-measures design (after adhering to a 36-hr exercise-dietary intervention to standardize preexercise muscle glycogen), eight males completed a 75-min nonexhaustive cycling protocol and consumed 22 g of a hydrolyzed collagen blend (COLLAGEN) or whey (WHEY) protein 45 min prior to exercise, 22 g during exercise, and 22 g immediately postexercise.
29757056	1	53	from	biogenesis	306:315	arg1	session					374:380	an acute training session	356:380	an acute training session completed with low carbohydrate availability	356:425	We examined the effects of whey versus collagen protein on skeletal muscle cell signaling responses associated with mitochondrial biogenesis and protein synthesis in recovery from an acute training session completed with low carbohydrate availability.
29757056	1	53	from	biogenesis	306:315	arg1	recovery					342:349	recovery	342:349	recovery from an acute training session completed with low carbohydrate availability	342:425	We examined the effects of whey versus collagen protein on skeletal muscle cell signaling responses associated with mitochondrial biogenesis and protein synthesis in recovery from an acute training session completed with low carbohydrate availability.
29757056	2	54	theme	collagen	656:663	arg1	COLLAGEN					672:679	a hydrolyzed collagen blend (COLLAGEN) or whey (WHEY) protein 45 min	643:710	COLLAGEN	672:679	In a repeated-measures design (after adhering to a 36-hr exercise-dietary intervention to standardize preexercise muscle glycogen), eight males completed a 75-min nonexhaustive cycling protocol and consumed 22 g of a hydrolyzed collagen blend (COLLAGEN) or whey (WHEY) protein 45 min prior to exercise, 22 g during exercise, and 22 g immediately postexercise.
29757056	7	55	theme	p70S6K1	1405:1411	arg1	activity					1413:1420	p70S6K1 activity	1405:1420	p70S6K1 activity	1405:1420	Postexercise feeding increased p70S6K1 activity at 1.5 hr postexercise (p < .05), the magnitude of which was greater (p < .05) in WHEY (180 ± 105 fmol·min-1·mg-1) versus COLLAGEN (73 ± 42 fmol·min-1·mg-1).
29757056	3	56	from	pre-	867:870	arg1	levels					855:860	comparable levels	844:860	comparable levels from pre- to postexercise in both trials (≈300-150 mmol/kg·dry weight)	844:931	Exercise decreased (p < .05) muscle glycogen content by comparable levels from pre- to postexercise in both trials (≈300-150 mmol/kg·dry weight).
29757056	4	57	theme	WHEY	934:937	arg1	protein					939:945	WHEY protein	934:945	WHEY protein	934:945	WHEY protein induced greater increases in plasma branched chain amino acids (p = .03) and leucine (p = .02) than COLLAGEN.
29757056	3	58	from	postexercise	875:886	arg1	trials					896:901	both trials	891:901	both trials (≈300-150 mmol/kg·dry weight)	891:931	Exercise decreased (p < .05) muscle glycogen content by comparable levels from pre- to postexercise in both trials (≈300-150 mmol/kg·dry weight).
29757056	3	58	from	postexercise	875:886	arg1	weight					925:930	≈300-150 mmol/kg·dry weight	904:930	≈300-150 mmol/kg·dry weight	904:930	Exercise decreased (p < .05) muscle glycogen content by comparable levels from pre- to postexercise in both trials (≈300-150 mmol/kg·dry weight).
29757056	6	59	theme	p70S6K1	1290:1296	arg1	activity					1298:1305	(p < .05) p70S6K1 activity	1280:1305	(p < .05) p70S6K1 activity	1280:1305	Exercise suppressed (p < .05) p70S6K1 activity in both conditions immediately postexercise (≈25 fmol·min-1·mg-1).
29757056	8	60	theme	low	1740:1742	arg1	availability					1757:1768	low carbohydrate availability	1740:1768	low carbohydrate availability	1740:1768	We conclude that protein composition does not modulate markers of mitochondrial biogenesis when in recovery from a training session deliberately completed with low carbohydrate availability.
29757056	0	61	theme	Carbohydrate	149:160	arg1	Availability					162:173	Low Carbohydrate Availability	145:173	Low Carbohydrate Availability	145:173	Whey Protein Augments Leucinemia and Postexercise p70S6K1 Activity Compared With a Hydrolyzed Collagen Blend When in Recovery From Training With Low Carbohydrate Availability.
29757056	5	62	theme	similar	1084:1090	arg1	increases					1092:1100	(p < .05) similar increases	1074:1100	(p < .05) similar increases in PGC-1α (fivefold) mRNA	1074:1126	Exercise induced (p < .05) similar increases in PGC-1α (fivefold) mRNA at 1.5 hr postexercise between conditions, although no effect of exercise (p > .05) was observed for p53, Parkin, and Beclin1 mRNA.
29757056	2	63	theme	cycling	605:611	arg1	protocol					613:620	a 75-min nonexhaustive cycling protocol	582:620	a 75-min nonexhaustive cycling protocol	582:620	In a repeated-measures design (after adhering to a 36-hr exercise-dietary intervention to standardize preexercise muscle glycogen), eight males completed a 75-min nonexhaustive cycling protocol and consumed 22 g of a hydrolyzed collagen blend (COLLAGEN) or whey (WHEY) protein 45 min prior to exercise, 22 g during exercise, and 22 g immediately postexercise.
29757056	3	64	theme	glycogen	824:831	arg1	content					833:839	muscle glycogen content	817:839	muscle glycogen content	817:839	Exercise decreased (p < .05) muscle glycogen content by comparable levels from pre- to postexercise in both trials (≈300-150 mmol/kg·dry weight).
29757056	1	65	from	effects	192:198	arg1	responses					266:274	skeletal muscle cell signaling responses	235:274	skeletal muscle cell signaling responses associated with mitochondrial biogenesis and protein synthesis in recovery from an acute training session completed with low carbohydrate availability	235:425	We examined the effects of whey versus collagen protein on skeletal muscle cell signaling responses associated with mitochondrial biogenesis and protein synthesis in recovery from an acute training session completed with low carbohydrate availability.
29757056	4	66	theme	chain	992:996	arg1	p = .03					1011:1017	p = .03	1011:1017	p = .03	1011:1017	WHEY protein induced greater increases in plasma branched chain amino acids (p = .03) and leucine (p = .02) than COLLAGEN.
29757056	4	66	theme	chain	992:996	arg1	acids					1004:1008	plasma branched chain amino acids	976:1008	plasma branched chain amino acids (p = .03)	976:1018	WHEY protein induced greater increases in plasma branched chain amino acids (p = .03) and leucine (p = .02) than COLLAGEN.
29757056	0	67	theme	Hydrolyzed	83:92	arg1	Blend					103:107	a Hydrolyzed Collagen Blend	81:107	a Hydrolyzed Collagen Blend When in Recovery From Training With Low Carbohydrate Availability	81:173	Whey Protein Augments Leucinemia and Postexercise p70S6K1 Activity Compared With a Hydrolyzed Collagen Blend When in Recovery From Training With Low Carbohydrate Availability.
29757056	2	68	theme	75-min	584:589	arg1	protocol					613:620	a 75-min nonexhaustive cycling protocol	582:620	a 75-min nonexhaustive cycling protocol	582:620	In a repeated-measures design (after adhering to a 36-hr exercise-dietary intervention to standardize preexercise muscle glycogen), eight males completed a 75-min nonexhaustive cycling protocol and consumed 22 g of a hydrolyzed collagen blend (COLLAGEN) or whey (WHEY) protein 45 min prior to exercise, 22 g during exercise, and 22 g immediately postexercise.
29757056	6	69	theme	postexercise	1338:1349	arg1	conditions					1315:1324	both conditions	1310:1324	both conditions immediately postexercise	1310:1349	Exercise suppressed (p < .05) p70S6K1 activity in both conditions immediately postexercise (≈25 fmol·min-1·mg-1).
29757056	1	70	theme	training	365:372	arg1	session					374:380	an acute training session	356:380	an acute training session completed with low carbohydrate availability	356:425	We examined the effects of whey versus collagen protein on skeletal muscle cell signaling responses associated with mitochondrial biogenesis and protein synthesis in recovery from an acute training session completed with low carbohydrate availability.
29757056	9	71	theme	whey	1784:1787	arg1	protein					1789:1795	whey protein	1784:1795	whey protein	1784:1795	By contrast, whey protein augments postexercise p70S6K activity compared with hydrolyzed collagen, as likely mediated via increased leucine availability.
29757056	4	72	theme	plasma	976:981	arg1	p = .03					1011:1017	p = .03	1011:1017	p = .03	1011:1017	WHEY protein induced greater increases in plasma branched chain amino acids (p = .03) and leucine (p = .02) than COLLAGEN.
29757056	4	72	theme	plasma	976:981	arg1	acids					1004:1008	plasma branched chain amino acids	976:1008	plasma branched chain amino acids (p = .03)	976:1018	WHEY protein induced greater increases in plasma branched chain amino acids (p = .03) and leucine (p = .02) than COLLAGEN.
29757056	2	73	theme	45 min	705:710	arg1	22 g					635:638	22 g	635:638	22 g of a hydrolyzed collagen blend (COLLAGEN) or whey (WHEY) protein 45 min prior to exercise, 22 g during exercise, and 22 g immediately postexercise	635:785	In a repeated-measures design (after adhering to a 36-hr exercise-dietary intervention to standardize preexercise muscle glycogen), eight males completed a 75-min nonexhaustive cycling protocol and consumed 22 g of a hydrolyzed collagen blend (COLLAGEN) or whey (WHEY) protein 45 min prior to exercise, 22 g during exercise, and 22 g immediately postexercise.
29757056	1	74	theme	low	397:399	arg1	availability					414:425	low carbohydrate availability	397:425	low carbohydrate availability	397:425	We examined the effects of whey versus collagen protein on skeletal muscle cell signaling responses associated with mitochondrial biogenesis and protein synthesis in recovery from an acute training session completed with low carbohydrate availability.
29757056	9	75	theme	p70S6K	1819:1824	arg1	activity					1826:1833	postexercise p70S6K activity	1806:1833	postexercise p70S6K activity	1806:1833	By contrast, whey protein augments postexercise p70S6K activity compared with hydrolyzed collagen, as likely mediated via increased leucine availability.
29757056	1	76	from	session	374:380	arg1	biogenesis					306:315	mitochondrial biogenesis	292:315	mitochondrial biogenesis	292:315	We examined the effects of whey versus collagen protein on skeletal muscle cell signaling responses associated with mitochondrial biogenesis and protein synthesis in recovery from an acute training session completed with low carbohydrate availability.
29757056	1	76	from	session	374:380	arg1	synthesis					329:337	protein synthesis	321:337	protein synthesis	321:337	We examined the effects of whey versus collagen protein on skeletal muscle cell signaling responses associated with mitochondrial biogenesis and protein synthesis in recovery from an acute training session completed with low carbohydrate availability.
29757056	1	76	from	session	374:380	arg1	recovery					342:349	recovery	342:349	recovery from an acute training session completed with low carbohydrate availability	342:425	We examined the effects of whey versus collagen protein on skeletal muscle cell signaling responses associated with mitochondrial biogenesis and protein synthesis in recovery from an acute training session completed with low carbohydrate availability.
29757056	1	77	theme	mitochondrial	292:304	arg1	biogenesis					306:315	mitochondrial biogenesis	292:315	mitochondrial biogenesis	292:315	We examined the effects of whey versus collagen protein on skeletal muscle cell signaling responses associated with mitochondrial biogenesis and protein synthesis in recovery from an acute training session completed with low carbohydrate availability.
29757056	0	78	theme	Postexercise	37:48	arg1	Activity					58:65	Postexercise p70S6K1 Activity	37:65	Postexercise p70S6K1 Activity	37:65	Whey Protein Augments Leucinemia and Postexercise p70S6K1 Activity Compared With a Hydrolyzed Collagen Blend When in Recovery From Training With Low Carbohydrate Availability.
29757056	5	79	theme	exercise	1193:1200	arg1	effect					1183:1188	no effect	1180:1188	no effect of exercise (p > .05)	1180:1210	Exercise induced (p < .05) similar increases in PGC-1α (fivefold) mRNA at 1.5 hr postexercise between conditions, although no effect of exercise (p > .05) was observed for p53, Parkin, and Beclin1 mRNA.
29757056	9	80	theme	hydrolyzed	1849:1858	arg1	collagen					1860:1867	hydrolyzed collagen	1849:1867	hydrolyzed collagen	1849:1867	By contrast, whey protein augments postexercise p70S6K activity compared with hydrolyzed collagen, as likely mediated via increased leucine availability.
29757056	8	81	from	session	1704:1710	arg1	recovery					1679:1686	recovery	1679:1686	recovery from a training session deliberately completed with low carbohydrate availability	1679:1768	We conclude that protein composition does not modulate markers of mitochondrial biogenesis when in recovery from a training session deliberately completed with low carbohydrate availability.
29757056	5	82	theme	PGC-1α	1105:1110	arg1	mRNA					1123:1126	PGC-1α (fivefold) mRNA	1105:1126	PGC-1α (fivefold) mRNA	1105:1126	Exercise induced (p < .05) similar increases in PGC-1α (fivefold) mRNA at 1.5 hr postexercise between conditions, although no effect of exercise (p > .05) was observed for p53, Parkin, and Beclin1 mRNA.
29757056	2	83	theme	36-hr	479:483	arg1	intervention					502:513	a 36-hr exercise-dietary intervention	477:513	a 36-hr exercise-dietary intervention to standardize preexercise muscle glycogen	477:556	In a repeated-measures design (after adhering to a 36-hr exercise-dietary intervention to standardize preexercise muscle glycogen), eight males completed a 75-min nonexhaustive cycling protocol and consumed 22 g of a hydrolyzed collagen blend (COLLAGEN) or whey (WHEY) protein 45 min prior to exercise, 22 g during exercise, and 22 g immediately postexercise.
29757056	8	84	theme	biogenesis	1660:1669	arg1	markers					1635:1641	markers	1635:1641	markers of mitochondrial biogenesis	1635:1669	We conclude that protein composition does not modulate markers of mitochondrial biogenesis when in recovery from a training session deliberately completed with low carbohydrate availability.
29757056	8	85	theme	protein	1597:1603	arg1	composition					1605:1615	protein composition	1597:1615	protein composition	1597:1615	We conclude that protein composition does not modulate markers of mitochondrial biogenesis when in recovery from a training session deliberately completed with low carbohydrate availability.
29757056	1	86	theme	muscle	244:249	arg1	responses					266:274	skeletal muscle cell signaling responses	235:274	skeletal muscle cell signaling responses associated with mitochondrial biogenesis and protein synthesis in recovery from an acute training session completed with low carbohydrate availability	235:425	We examined the effects of whey versus collagen protein on skeletal muscle cell signaling responses associated with mitochondrial biogenesis and protein synthesis in recovery from an acute training session completed with low carbohydrate availability.
29957055	7	0	theme	molecular	1430:1438	arg1	mechanisms					1440:1449	molecular mechanisms	1430:1449	molecular mechanisms involved in the progression toward T2DM manifestation.-Paschen, M., Moede, T., Valladolid-Acebes, I., Leibiger, B., Moruzzi, N., Jacob, S., García-Prieto, C. F.	1430:1610	Employing the in vivo reporter islet-monitoring approach will allow researchers to define key times in the dynamics of reversible loss of functional β-cell mass and, thus, to investigate the underlying, molecular mechanisms involved in the progression toward T2DM manifestation.-Paschen, M., Moede, T., Valladolid-Acebes, I., Leibiger, B., Moruzzi, N., Jacob, S., García-Prieto, C. F., Brismar, K., Leibiger, I. B., Berggren, P.-O.
29957055	7	0	theme	molecular	1430:1438	arg1	B.					1639:1640	B.	1639:1640	B.	1639:1640	Employing the in vivo reporter islet-monitoring approach will allow researchers to define key times in the dynamics of reversible loss of functional β-cell mass and, thus, to investigate the underlying, molecular mechanisms involved in the progression toward T2DM manifestation.-Paschen, M., Moede, T., Valladolid-Acebes, I., Leibiger, B., Moruzzi, N., Jacob, S., García-Prieto, C. F., Brismar, K., Leibiger, I. B., Berggren, P.-O.
29957055	7	0	theme	molecular	1430:1438	arg1	P.-O					1653:1656	P.-O	1653:1656	P.-O	1653:1656	Employing the in vivo reporter islet-monitoring approach will allow researchers to define key times in the dynamics of reversible loss of functional β-cell mass and, thus, to investigate the underlying, molecular mechanisms involved in the progression toward T2DM manifestation.-Paschen, M., Moede, T., Valladolid-Acebes, I., Leibiger, B., Moruzzi, N., Jacob, S., García-Prieto, C. F., Brismar, K., Leibiger, I. B., Berggren, P.-O.
29957055	4	1	theme	concomitant	814:824	arg1	loss					826:829	a concomitant loss	812:829	a concomitant loss of functional β-cell mass	812:855	Through reporter islets transplanted to the anterior chamber of the eye, we demonstrated a concomitant loss of functional β-cell mass.
29957055	7	2	theme	underlying	1418:1427	arg1	mechanisms					1440:1449	molecular mechanisms	1430:1449	molecular mechanisms involved in the progression toward T2DM manifestation.-Paschen, M., Moede, T., Valladolid-Acebes, I., Leibiger, B., Moruzzi, N., Jacob, S., García-Prieto, C. F.	1430:1610	Employing the in vivo reporter islet-monitoring approach will allow researchers to define key times in the dynamics of reversible loss of functional β-cell mass and, thus, to investigate the underlying, molecular mechanisms involved in the progression toward T2DM manifestation.-Paschen, M., Moede, T., Valladolid-Acebes, I., Leibiger, B., Moruzzi, N., Jacob, S., García-Prieto, C. F., Brismar, K., Leibiger, I. B., Berggren, P.-O.
29957055	7	2	theme	underlying	1418:1427	arg1	B.					1639:1640	B.	1639:1640	B.	1639:1640	Employing the in vivo reporter islet-monitoring approach will allow researchers to define key times in the dynamics of reversible loss of functional β-cell mass and, thus, to investigate the underlying, molecular mechanisms involved in the progression toward T2DM manifestation.-Paschen, M., Moede, T., Valladolid-Acebes, I., Leibiger, B., Moruzzi, N., Jacob, S., García-Prieto, C. F., Brismar, K., Leibiger, I. B., Berggren, P.-O.
29957055	7	2	theme	underlying	1418:1427	arg1	P.-O					1653:1656	P.-O	1653:1656	P.-O	1653:1656	Employing the in vivo reporter islet-monitoring approach will allow researchers to define key times in the dynamics of reversible loss of functional β-cell mass and, thus, to investigate the underlying, molecular mechanisms involved in the progression toward T2DM manifestation.-Paschen, M., Moede, T., Valladolid-Acebes, I., Leibiger, B., Moruzzi, N., Jacob, S., García-Prieto, C. F., Brismar, K., Leibiger, I. B., Berggren, P.-O.
29957055	8	3	theme	functional	1728:1737	arg1	mass					1746:1749	functional β-cell mass	1728:1749	functional β-cell mass	1728:1749	Diet-induced β-cell insulin resistance results in reversible loss of functional β-cell mass.
29957055	7	4	theme	β-cell	1376:1381	arg1	mass					1383:1386	functional β-cell mass	1365:1386	functional β-cell mass	1365:1386	Employing the in vivo reporter islet-monitoring approach will allow researchers to define key times in the dynamics of reversible loss of functional β-cell mass and, thus, to investigate the underlying, molecular mechanisms involved in the progression toward T2DM manifestation.-Paschen, M., Moede, T., Valladolid-Acebes, I., Leibiger, B., Moruzzi, N., Jacob, S., García-Prieto, C. F., Brismar, K., Leibiger, I. B., Berggren, P.-O.
29957055	6	5	theme	given	1201:1205	arg1	background					1215:1224	a given genetic background	1199:1224	a given genetic background	1199:1224	Our data reinforce the relevance of diet composition as an environmental factor determining different routes of diabetes progression in a given genetic background.
29957055	2	6	theme	male	262:265	arg1	mice					277:280	male, C57B1/6J mice	262:280	male, C57B1/6J mice	262:280	Exemplified by diabetes-prone, male, C57B1/6J mice being fed different combinations of Western-style diet, we show that β-cell insulin resistance occurs early during T2DM progression and is due to a combination of lipotoxicity and increased β-cell workload.
29957055	2	7	theme	different	292:300	arg1	combinations					302:313	fed different combinations	288:313	fed different combinations of Western-style diet	288:335	Exemplified by diabetes-prone, male, C57B1/6J mice being fed different combinations of Western-style diet, we show that β-cell insulin resistance occurs early during T2DM progression and is due to a combination of lipotoxicity and increased β-cell workload.
29957055	1	8	theme	type	191:194	arg1	diabetes					198:205	diet-induced type 2 diabetes (T2DM)	178:212	diet-induced type 2 diabetes (T2DM)	178:212	Although convincing in genetic models, the relevance of β-cell insulin resistance in diet-induced type 2 diabetes (T2DM) remains unclear.
29957055	1	8	theme	type	191:194	arg1	T2DM					208:211	T2DM	208:211	T2DM	208:211	Although convincing in genetic models, the relevance of β-cell insulin resistance in diet-induced type 2 diabetes (T2DM) remains unclear.
29957055	7	9	theme	reversible	1346:1355	arg1	loss					1357:1360	reversible loss	1346:1360	reversible loss of functional β-cell mass and, thus, to investigate the underlying, molecular mechanisms involved in the progression toward T2DM manifestation.-Paschen, M., Moede, T., Valladolid-Acebes, I., Leibiger, B., Moruzzi, N., Jacob, S., García-Prieto, C. F., Brismar, K., Leibiger, I. B., Berggren, P.-O	1346:1656	Employing the in vivo reporter islet-monitoring approach will allow researchers to define key times in the dynamics of reversible loss of functional β-cell mass and, thus, to investigate the underlying, molecular mechanisms involved in the progression toward T2DM manifestation.-Paschen, M., Moede, T., Valladolid-Acebes, I., Leibiger, B., Moruzzi, N., Jacob, S., García-Prieto, C. F., Brismar, K., Leibiger, I. B., Berggren, P.-O.
29957055	5	10	theme	β-cell	1011:1016	arg1	mass					1018:1021	functional β-cell mass	1000:1021	functional β-cell mass	1000:1021	When mice were changed from diabetogenic diet to normal chow diet, the diabetes phenotype was reversed, suggesting a remarkable plasticity of functional β-cell mass in the early phase of T2DM development.
29957055	3	11	theme	high-sucrose	526:537	arg1	high-fat					516:523	a high-fat	514:523	a high-fat	514:523	Within 8 wk of being fed a high-fat, high-sucrose diet, mice became obese, developed impaired insulin and glucose tolerances, and displayed noncompensatory insulin release, due, at least in part, to reduced expression of syntaxin-1A.
29957055	3	11	theme	high-sucrose	526:537	arg1	diet					539:542	high-sucrose diet	526:542	high-sucrose diet	526:542	Within 8 wk of being fed a high-fat, high-sucrose diet, mice became obese, developed impaired insulin and glucose tolerances, and displayed noncompensatory insulin release, due, at least in part, to reduced expression of syntaxin-1A.
29957055	6	12	theme	progression	1184:1194	arg1	routes					1165:1170	different routes	1155:1170	different routes of diabetes progression	1155:1194	Our data reinforce the relevance of diet composition as an environmental factor determining different routes of diabetes progression in a given genetic background.
29957055	4	13	theme	β-cell	845:850	arg1	mass					852:855	functional β-cell mass	834:855	functional β-cell mass	834:855	Through reporter islets transplanted to the anterior chamber of the eye, we demonstrated a concomitant loss of functional β-cell mass.
29957055	3	14	theme	syntaxin-1A	710:720	arg1	expression					696:705	reduced expression	688:705	reduced expression of syntaxin-1A	688:720	Within 8 wk of being fed a high-fat, high-sucrose diet, mice became obese, developed impaired insulin and glucose tolerances, and displayed noncompensatory insulin release, due, at least in part, to reduced expression of syntaxin-1A.
29957055	5	15	theme	remarkable	975:984	arg1	plasticity					986:995	a remarkable plasticity	973:995	a remarkable plasticity of functional β-cell mass in the early phase of T2DM development	973:1060	When mice were changed from diabetogenic diet to normal chow diet, the diabetes phenotype was reversed, suggesting a remarkable plasticity of functional β-cell mass in the early phase of T2DM development.
29957055	6	16	theme	different	1155:1163	arg1	routes					1165:1170	different routes	1155:1170	different routes of diabetes progression	1155:1194	Our data reinforce the relevance of diet composition as an environmental factor determining different routes of diabetes progression in a given genetic background.
29957055	7	17	theme	key	1317:1319	arg1	times					1321:1325	key times	1317:1325	key times	1317:1325	Employing the in vivo reporter islet-monitoring approach will allow researchers to define key times in the dynamics of reversible loss of functional β-cell mass and, thus, to investigate the underlying, molecular mechanisms involved in the progression toward T2DM manifestation.-Paschen, M., Moede, T., Valladolid-Acebes, I., Leibiger, B., Moruzzi, N., Jacob, S., García-Prieto, C. F., Brismar, K., Leibiger, I. B., Berggren, P.-O.
29957055	5	18	theme	development	1050:1060	arg1	phase					1036:1040	the early phase	1026:1040	the early phase of T2DM development	1026:1060	When mice were changed from diabetogenic diet to normal chow diet, the diabetes phenotype was reversed, suggesting a remarkable plasticity of functional β-cell mass in the early phase of T2DM development.
29957055	3	19	theme	impaired	574:581	arg1	insulin					583:589	impaired insulin	574:589	impaired insulin	574:589	Within 8 wk of being fed a high-fat, high-sucrose diet, mice became obese, developed impaired insulin and glucose tolerances, and displayed noncompensatory insulin release, due, at least in part, to reduced expression of syntaxin-1A.
29957055	2	20	theme	β-cell	472:477	arg1	workload					479:486	increased β-cell workload	462:486	increased β-cell workload	462:486	Exemplified by diabetes-prone, male, C57B1/6J mice being fed different combinations of Western-style diet, we show that β-cell insulin resistance occurs early during T2DM progression and is due to a combination of lipotoxicity and increased β-cell workload.
29957055	5	21	from	plasticity	986:995	arg1	phase					1036:1040	the early phase	1026:1040	the early phase of T2DM development	1026:1060	When mice were changed from diabetogenic diet to normal chow diet, the diabetes phenotype was reversed, suggesting a remarkable plasticity of functional β-cell mass in the early phase of T2DM development.
29957055	8	22	theme	Diet-induced	1659:1670	arg1	resistance					1687:1696	Diet-induced β-cell insulin resistance	1659:1696	Diet-induced β-cell insulin resistance	1659:1696	Diet-induced β-cell insulin resistance results in reversible loss of functional β-cell mass.
29957055	1	23	theme	insulin	156:162	arg1	resistance					164:173	β-cell insulin resistance	149:173	β-cell insulin resistance	149:173	Although convincing in genetic models, the relevance of β-cell insulin resistance in diet-induced type 2 diabetes (T2DM) remains unclear.
29957055	5	24	theme	early	1030:1034	arg1	phase					1036:1040	the early phase	1026:1040	the early phase of T2DM development	1026:1060	When mice were changed from diabetogenic diet to normal chow diet, the diabetes phenotype was reversed, suggesting a remarkable plasticity of functional β-cell mass in the early phase of T2DM development.
29957055	1	25	from	relevance	136:144	arg1	diabetes					198:205	diet-induced type 2 diabetes (T2DM)	178:212	diet-induced type 2 diabetes (T2DM)	178:212	Although convincing in genetic models, the relevance of β-cell insulin resistance in diet-induced type 2 diabetes (T2DM) remains unclear.
29957055	1	25	from	relevance	136:144	arg1	T2DM					208:211	T2DM	208:211	T2DM	208:211	Although convincing in genetic models, the relevance of β-cell insulin resistance in diet-induced type 2 diabetes (T2DM) remains unclear.
29957055	5	26	theme	chow	914:917	arg1	diet					919:922	normal chow diet	907:922	normal chow diet	907:922	When mice were changed from diabetogenic diet to normal chow diet, the diabetes phenotype was reversed, suggesting a remarkable plasticity of functional β-cell mass in the early phase of T2DM development.
29957055	4	27	theme	reporter	731:738	arg1	islets					740:745	reporter islets	731:745	reporter islets transplanted to the anterior chamber of the eye	731:793	Through reporter islets transplanted to the anterior chamber of the eye, we demonstrated a concomitant loss of functional β-cell mass.
29957055	3	28	from	expression	696:705	arg1	part					679:682	part	679:682	part	679:682	Within 8 wk of being fed a high-fat, high-sucrose diet, mice became obese, developed impaired insulin and glucose tolerances, and displayed noncompensatory insulin release, due, at least in part, to reduced expression of syntaxin-1A.
29957055	0	29	theme	Diet-induced	0:11	arg1	resistance					28:37	Diet-induced β-cell insulin resistance	0:37	Diet-induced β-cell insulin resistance	0:37	Diet-induced β-cell insulin resistance results in reversible loss of functional β-cell mass.
29957055	8	30	theme	reversible	1709:1718	arg1	loss					1720:1723	reversible loss	1709:1723	reversible loss of functional β-cell mass	1709:1749	Diet-induced β-cell insulin resistance results in reversible loss of functional β-cell mass.
29957055	5	31	theme	diabetogenic	886:897	arg1	diet					899:902	diabetogenic diet	886:902	diabetogenic diet to normal chow diet	886:922	When mice were changed from diabetogenic diet to normal chow diet, the diabetes phenotype was reversed, suggesting a remarkable plasticity of functional β-cell mass in the early phase of T2DM development.
29957055	0	32	theme	insulin	20:26	arg1	resistance					28:37	Diet-induced β-cell insulin resistance	0:37	Diet-induced β-cell insulin resistance	0:37	Diet-induced β-cell insulin resistance results in reversible loss of functional β-cell mass.
29957055	6	33	theme	composition	1104:1114	arg1	relevance					1086:1094	the relevance	1082:1094	the relevance of diet composition	1082:1114	Our data reinforce the relevance of diet composition as an environmental factor determining different routes of diabetes progression in a given genetic background.
29957055	6	33	theme	composition	1104:1114	arg1	factor					1136:1141	an environmental factor	1119:1141	an environmental factor determining different routes of diabetes progression in a given genetic background	1119:1224	Our data reinforce the relevance of diet composition as an environmental factor determining different routes of diabetes progression in a given genetic background.
29957055	7	34	theme	T2DM	1486:1489	arg1	F.					1609:1610	F.	1609:1610	F.	1609:1610	Employing the in vivo reporter islet-monitoring approach will allow researchers to define key times in the dynamics of reversible loss of functional β-cell mass and, thus, to investigate the underlying, molecular mechanisms involved in the progression toward T2DM manifestation.-Paschen, M., Moede, T., Valladolid-Acebes, I., Leibiger, B., Moruzzi, N., Jacob, S., García-Prieto, C. F., Brismar, K., Leibiger, I. B., Berggren, P.-O.
29957055	7	34	theme	T2DM	1486:1489	arg1	manifestation.-Paschen					1491:1512	T2DM manifestation.-Paschen	1486:1512	T2DM manifestation.-Paschen	1486:1512	Employing the in vivo reporter islet-monitoring approach will allow researchers to define key times in the dynamics of reversible loss of functional β-cell mass and, thus, to investigate the underlying, molecular mechanisms involved in the progression toward T2DM manifestation.-Paschen, M., Moede, T., Valladolid-Acebes, I., Leibiger, B., Moruzzi, N., Jacob, S., García-Prieto, C. F., Brismar, K., Leibiger, I. B., Berggren, P.-O.
29957055	7	35	theme	islet-monitoring	1258:1273	arg1	approach					1275:1282	the in vivo reporter islet-monitoring approach	1237:1282	the in vivo reporter islet-monitoring approach	1237:1282	Employing the in vivo reporter islet-monitoring approach will allow researchers to define key times in the dynamics of reversible loss of functional β-cell mass and, thus, to investigate the underlying, molecular mechanisms involved in the progression toward T2DM manifestation.-Paschen, M., Moede, T., Valladolid-Acebes, I., Leibiger, B., Moruzzi, N., Jacob, S., García-Prieto, C. F., Brismar, K., Leibiger, I. B., Berggren, P.-O.
29957055	2	36	theme	T2DM	397:400	arg1	progression					402:412	T2DM progression	397:412	T2DM progression	397:412	Exemplified by diabetes-prone, male, C57B1/6J mice being fed different combinations of Western-style diet, we show that β-cell insulin resistance occurs early during T2DM progression and is due to a combination of lipotoxicity and increased β-cell workload.
29957055	3	37	dep	mice	545:548	arg1	displayed					619:627	displayed	619:627	displayed noncompensatory insulin release	619:659	Within 8 wk of being fed a high-fat, high-sucrose diet, mice became obese, developed impaired insulin and glucose tolerances, and displayed noncompensatory insulin release, due, at least in part, to reduced expression of syntaxin-1A.
29957055	3	37	dep	mice	545:548	arg1	developed					564:572	developed	564:572	developed impaired insulin and glucose tolerances	564:612	Within 8 wk of being fed a high-fat, high-sucrose diet, mice became obese, developed impaired insulin and glucose tolerances, and displayed noncompensatory insulin release, due, at least in part, to reduced expression of syntaxin-1A.
29957055	3	37	dep	mice	545:548	arg1	became					550:555	became	550:555	became obese	550:561	Within 8 wk of being fed a high-fat, high-sucrose diet, mice became obese, developed impaired insulin and glucose tolerances, and displayed noncompensatory insulin release, due, at least in part, to reduced expression of syntaxin-1A.
29957055	7	38	dep	thus	1393:1396	arg1	investigate					1402:1412	investigate	1402:1412	to investigate the underlying, molecular mechanisms involved in the progression toward T2DM manifestation.-Paschen, M., Moede, T., Valladolid-Acebes, I., Leibiger, B., Moruzzi, N., Jacob, S., García-Prieto, C. F., Brismar, K., Leibiger, I. B., Berggren, P.-O	1399:1656	Employing the in vivo reporter islet-monitoring approach will allow researchers to define key times in the dynamics of reversible loss of functional β-cell mass and, thus, to investigate the underlying, molecular mechanisms involved in the progression toward T2DM manifestation.-Paschen, M., Moede, T., Valladolid-Acebes, I., Leibiger, B., Moruzzi, N., Jacob, S., García-Prieto, C. F., Brismar, K., Leibiger, I. B., Berggren, P.-O.
29957055	0	39	theme	reversible	50:59	arg1	loss					61:64	reversible loss	50:64	reversible loss of functional β-cell mass	50:90	Diet-induced β-cell insulin resistance results in reversible loss of functional β-cell mass.
29957055	8	40	theme	mass	1746:1749	arg1	loss					1720:1723	reversible loss	1709:1723	reversible loss of functional β-cell mass	1709:1749	Diet-induced β-cell insulin resistance results in reversible loss of functional β-cell mass.
29957055	2	41	theme	Western-style	318:330	arg1	diet					332:335	Western-style diet	318:335	Western-style diet	318:335	Exemplified by diabetes-prone, male, C57B1/6J mice being fed different combinations of Western-style diet, we show that β-cell insulin resistance occurs early during T2DM progression and is due to a combination of lipotoxicity and increased β-cell workload.
29957055	7	42	theme	mass	1383:1386	arg1	loss					1357:1360	reversible loss	1346:1360	reversible loss of functional β-cell mass and, thus, to investigate the underlying, molecular mechanisms involved in the progression toward T2DM manifestation.-Paschen, M., Moede, T., Valladolid-Acebes, I., Leibiger, B., Moruzzi, N., Jacob, S., García-Prieto, C. F., Brismar, K., Leibiger, I. B., Berggren, P.-O	1346:1656	Employing the in vivo reporter islet-monitoring approach will allow researchers to define key times in the dynamics of reversible loss of functional β-cell mass and, thus, to investigate the underlying, molecular mechanisms involved in the progression toward T2DM manifestation.-Paschen, M., Moede, T., Valladolid-Acebes, I., Leibiger, B., Moruzzi, N., Jacob, S., García-Prieto, C. F., Brismar, K., Leibiger, I. B., Berggren, P.-O.
29957055	5	43	theme	diabetes	929:936	arg1	phenotype					938:946	the diabetes phenotype	925:946	the diabetes phenotype	925:946	When mice were changed from diabetogenic diet to normal chow diet, the diabetes phenotype was reversed, suggesting a remarkable plasticity of functional β-cell mass in the early phase of T2DM development.
29957055	3	44	theme	noncompensatory	629:643	arg1	release					653:659	noncompensatory insulin release	629:659	noncompensatory insulin release	629:659	Within 8 wk of being fed a high-fat, high-sucrose diet, mice became obese, developed impaired insulin and glucose tolerances, and displayed noncompensatory insulin release, due, at least in part, to reduced expression of syntaxin-1A.
29957055	2	45	theme	β-cell	351:356	arg1	resistance					366:375	β-cell insulin resistance	351:375	β-cell insulin resistance	351:375	Exemplified by diabetes-prone, male, C57B1/6J mice being fed different combinations of Western-style diet, we show that β-cell insulin resistance occurs early during T2DM progression and is due to a combination of lipotoxicity and increased β-cell workload.
29957055	8	46	theme	β-cell	1739:1744	arg1	mass					1746:1749	functional β-cell mass	1728:1749	functional β-cell mass	1728:1749	Diet-induced β-cell insulin resistance results in reversible loss of functional β-cell mass.
29957055	6	47	theme	genetic	1207:1213	arg1	background					1215:1224	a given genetic background	1199:1224	a given genetic background	1199:1224	Our data reinforce the relevance of diet composition as an environmental factor determining different routes of diabetes progression in a given genetic background.
29957055	1	48	theme	resistance	164:173	arg1	relevance					136:144	the relevance	132:144	the relevance of β-cell insulin resistance in diet-induced type 2 diabetes (T2DM)	132:212	Although convincing in genetic models, the relevance of β-cell insulin resistance in diet-induced type 2 diabetes (T2DM) remains unclear.
29957055	7	49	theme	thus	1393:1396	arg1	loss					1357:1360	reversible loss	1346:1360	reversible loss of functional β-cell mass and, thus, to investigate the underlying, molecular mechanisms involved in the progression toward T2DM manifestation.-Paschen, M., Moede, T., Valladolid-Acebes, I., Leibiger, B., Moruzzi, N., Jacob, S., García-Prieto, C. F., Brismar, K., Leibiger, I. B., Berggren, P.-O	1346:1656	Employing the in vivo reporter islet-monitoring approach will allow researchers to define key times in the dynamics of reversible loss of functional β-cell mass and, thus, to investigate the underlying, molecular mechanisms involved in the progression toward T2DM manifestation.-Paschen, M., Moede, T., Valladolid-Acebes, I., Leibiger, B., Moruzzi, N., Jacob, S., García-Prieto, C. F., Brismar, K., Leibiger, I. B., Berggren, P.-O.
29957055	4	50	theme	eye	791:793	arg1	chamber					776:782	the anterior chamber	763:782	the anterior chamber of the eye	763:793	Through reporter islets transplanted to the anterior chamber of the eye, we demonstrated a concomitant loss of functional β-cell mass.
29957055	6	51	theme	diet	1099:1102	arg1	composition					1104:1114	diet composition	1099:1114	diet composition	1099:1114	Our data reinforce the relevance of diet composition as an environmental factor determining different routes of diabetes progression in a given genetic background.
29957055	2	52	dep	Exemplified	231:241	arg1	combinations					302:313	fed different combinations	288:313	fed different combinations of Western-style diet	288:335	Exemplified by diabetes-prone, male, C57B1/6J mice being fed different combinations of Western-style diet, we show that β-cell insulin resistance occurs early during T2DM progression and is due to a combination of lipotoxicity and increased β-cell workload.
29957055	0	53	theme	β-cell	80:85	arg1	mass					87:90	functional β-cell mass	69:90	functional β-cell mass	69:90	Diet-induced β-cell insulin resistance results in reversible loss of functional β-cell mass.
29957055	1	54	theme	diet-induced	178:189	arg1	diabetes					198:205	diet-induced type 2 diabetes (T2DM)	178:212	diet-induced type 2 diabetes (T2DM)	178:212	Although convincing in genetic models, the relevance of β-cell insulin resistance in diet-induced type 2 diabetes (T2DM) remains unclear.
29957055	1	54	theme	diet-induced	178:189	arg1	T2DM					208:211	T2DM	208:211	T2DM	208:211	Although convincing in genetic models, the relevance of β-cell insulin resistance in diet-induced type 2 diabetes (T2DM) remains unclear.
29957055	7	55	theme	functional	1365:1374	arg1	mass					1383:1386	functional β-cell mass	1365:1386	functional β-cell mass	1365:1386	Employing the in vivo reporter islet-monitoring approach will allow researchers to define key times in the dynamics of reversible loss of functional β-cell mass and, thus, to investigate the underlying, molecular mechanisms involved in the progression toward T2DM manifestation.-Paschen, M., Moede, T., Valladolid-Acebes, I., Leibiger, B., Moruzzi, N., Jacob, S., García-Prieto, C. F., Brismar, K., Leibiger, I. B., Berggren, P.-O.
29957055	2	56	theme	fed	288:290	arg1	combinations					302:313	fed different combinations	288:313	fed different combinations of Western-style diet	288:335	Exemplified by diabetes-prone, male, C57B1/6J mice being fed different combinations of Western-style diet, we show that β-cell insulin resistance occurs early during T2DM progression and is due to a combination of lipotoxicity and increased β-cell workload.
29957055	7	57	theme	loss	1357:1360	arg1	dynamics					1334:1341	the dynamics	1330:1341	the dynamics of reversible loss of functional β-cell mass and, thus, to investigate the underlying, molecular mechanisms involved in the progression toward T2DM manifestation.-Paschen, M., Moede, T., Valladolid-Acebes, I., Leibiger, B., Moruzzi, N., Jacob, S., García-Prieto, C. F., Brismar, K., Leibiger, I. B., Berggren, P.-O	1330:1656	Employing the in vivo reporter islet-monitoring approach will allow researchers to define key times in the dynamics of reversible loss of functional β-cell mass and, thus, to investigate the underlying, molecular mechanisms involved in the progression toward T2DM manifestation.-Paschen, M., Moede, T., Valladolid-Acebes, I., Leibiger, B., Moruzzi, N., Jacob, S., García-Prieto, C. F., Brismar, K., Leibiger, I. B., Berggren, P.-O.
29957055	3	58	theme	reduced	688:694	arg1	expression					696:705	reduced expression	688:705	reduced expression of syntaxin-1A	688:720	Within 8 wk of being fed a high-fat, high-sucrose diet, mice became obese, developed impaired insulin and glucose tolerances, and displayed noncompensatory insulin release, due, at least in part, to reduced expression of syntaxin-1A.
29957055	5	59	theme	mass	1018:1021	arg1	plasticity					986:995	a remarkable plasticity	973:995	a remarkable plasticity of functional β-cell mass in the early phase of T2DM development	973:1060	When mice were changed from diabetogenic diet to normal chow diet, the diabetes phenotype was reversed, suggesting a remarkable plasticity of functional β-cell mass in the early phase of T2DM development.
29957055	6	60	theme	diabetes	1175:1182	arg1	progression					1184:1194	diabetes progression	1175:1194	diabetes progression	1175:1194	Our data reinforce the relevance of diet composition as an environmental factor determining different routes of diabetes progression in a given genetic background.
29957055	1	61	theme	genetic	116:122	arg1	models					124:129	genetic models	116:129	genetic models	116:129	Although convincing in genetic models, the relevance of β-cell insulin resistance in diet-induced type 2 diabetes (T2DM) remains unclear.
29957055	4	62	theme	functional	834:843	arg1	mass					852:855	functional β-cell mass	834:855	functional β-cell mass	834:855	Through reporter islets transplanted to the anterior chamber of the eye, we demonstrated a concomitant loss of functional β-cell mass.
29957055	4	63	theme	mass	852:855	arg1	loss					826:829	a concomitant loss	812:829	a concomitant loss of functional β-cell mass	812:855	Through reporter islets transplanted to the anterior chamber of the eye, we demonstrated a concomitant loss of functional β-cell mass.
29957055	0	64	theme	functional	69:78	arg1	mass					87:90	functional β-cell mass	69:90	functional β-cell mass	69:90	Diet-induced β-cell insulin resistance results in reversible loss of functional β-cell mass.
29957055	5	65	theme	functional	1000:1009	arg1	mass					1018:1021	functional β-cell mass	1000:1021	functional β-cell mass	1000:1021	When mice were changed from diabetogenic diet to normal chow diet, the diabetes phenotype was reversed, suggesting a remarkable plasticity of functional β-cell mass in the early phase of T2DM development.
29957055	2	66	theme	workload	479:486	arg1	combination					430:440	a combination	428:440	a combination of lipotoxicity and increased β-cell workload	428:486	Exemplified by diabetes-prone, male, C57B1/6J mice being fed different combinations of Western-style diet, we show that β-cell insulin resistance occurs early during T2DM progression and is due to a combination of lipotoxicity and increased β-cell workload.
29957055	6	67	theme	environmental	1122:1134	arg1	relevance					1086:1094	the relevance	1082:1094	the relevance of diet composition	1082:1114	Our data reinforce the relevance of diet composition as an environmental factor determining different routes of diabetes progression in a given genetic background.
29957055	6	67	theme	environmental	1122:1134	arg1	factor					1136:1141	an environmental factor	1119:1141	an environmental factor determining different routes of diabetes progression in a given genetic background	1119:1224	Our data reinforce the relevance of diet composition as an environmental factor determining different routes of diabetes progression in a given genetic background.
29957055	2	68	dep	male	262:265	arg1	C57B1/6J					268:275	C57B1/6J	268:275	C57B1/6J	268:275	Exemplified by diabetes-prone, male, C57B1/6J mice being fed different combinations of Western-style diet, we show that β-cell insulin resistance occurs early during T2DM progression and is due to a combination of lipotoxicity and increased β-cell workload.
29957055	0	69	theme	mass	87:90	arg1	loss					61:64	reversible loss	50:64	reversible loss of functional β-cell mass	50:90	Diet-induced β-cell insulin resistance results in reversible loss of functional β-cell mass.
29957055	1	70	theme	β-cell	149:154	arg1	resistance					164:173	β-cell insulin resistance	149:173	β-cell insulin resistance	149:173	Although convincing in genetic models, the relevance of β-cell insulin resistance in diet-induced type 2 diabetes (T2DM) remains unclear.
29957055	2	71	theme	increased	462:470	arg1	workload					479:486	increased β-cell workload	462:486	increased β-cell workload	462:486	Exemplified by diabetes-prone, male, C57B1/6J mice being fed different combinations of Western-style diet, we show that β-cell insulin resistance occurs early during T2DM progression and is due to a combination of lipotoxicity and increased β-cell workload.
29957055	8	72	theme	insulin	1679:1685	arg1	resistance					1687:1696	Diet-induced β-cell insulin resistance	1659:1696	Diet-induced β-cell insulin resistance	1659:1696	Diet-induced β-cell insulin resistance results in reversible loss of functional β-cell mass.
29957055	5	73	theme	normal	907:912	arg1	diet					919:922	normal chow diet	907:922	normal chow diet	907:922	When mice were changed from diabetogenic diet to normal chow diet, the diabetes phenotype was reversed, suggesting a remarkable plasticity of functional β-cell mass in the early phase of T2DM development.
29957055	8	74	theme	β-cell	1672:1677	arg1	resistance					1687:1696	Diet-induced β-cell insulin resistance	1659:1696	Diet-induced β-cell insulin resistance	1659:1696	Diet-induced β-cell insulin resistance results in reversible loss of functional β-cell mass.
29957055	0	75	theme	β-cell	13:18	arg1	resistance					28:37	Diet-induced β-cell insulin resistance	0:37	Diet-induced β-cell insulin resistance	0:37	Diet-induced β-cell insulin resistance results in reversible loss of functional β-cell mass.
29957055	5	76	theme	T2DM	1045:1048	arg1	development					1050:1060	T2DM development	1045:1060	T2DM development	1045:1060	When mice were changed from diabetogenic diet to normal chow diet, the diabetes phenotype was reversed, suggesting a remarkable plasticity of functional β-cell mass in the early phase of T2DM development.
29957055	2	77	theme	insulin	358:364	arg1	resistance					366:375	β-cell insulin resistance	351:375	β-cell insulin resistance	351:375	Exemplified by diabetes-prone, male, C57B1/6J mice being fed different combinations of Western-style diet, we show that β-cell insulin resistance occurs early during T2DM progression and is due to a combination of lipotoxicity and increased β-cell workload.
29957055	7	78	theme	reporter	1249:1256	arg1	approach					1275:1282	the in vivo reporter islet-monitoring approach	1237:1282	the in vivo reporter islet-monitoring approach	1237:1282	Employing the in vivo reporter islet-monitoring approach will allow researchers to define key times in the dynamics of reversible loss of functional β-cell mass and, thus, to investigate the underlying, molecular mechanisms involved in the progression toward T2DM manifestation.-Paschen, M., Moede, T., Valladolid-Acebes, I., Leibiger, B., Moruzzi, N., Jacob, S., García-Prieto, C. F., Brismar, K., Leibiger, I. B., Berggren, P.-O.
29957055	7	79	theme	in	1241:1242	arg1	approach					1275:1282	the in vivo reporter islet-monitoring approach	1237:1282	the in vivo reporter islet-monitoring approach	1237:1282	Employing the in vivo reporter islet-monitoring approach will allow researchers to define key times in the dynamics of reversible loss of functional β-cell mass and, thus, to investigate the underlying, molecular mechanisms involved in the progression toward T2DM manifestation.-Paschen, M., Moede, T., Valladolid-Acebes, I., Leibiger, B., Moruzzi, N., Jacob, S., García-Prieto, C. F., Brismar, K., Leibiger, I. B., Berggren, P.-O.
29957055	2	80	theme	lipotoxicity	445:456	arg1	combination					430:440	a combination	428:440	a combination of lipotoxicity and increased β-cell workload	428:486	Exemplified by diabetes-prone, male, C57B1/6J mice being fed different combinations of Western-style diet, we show that β-cell insulin resistance occurs early during T2DM progression and is due to a combination of lipotoxicity and increased β-cell workload.
29957055	3	81	theme	glucose	595:601	arg1	tolerances					603:612	glucose tolerances	595:612	glucose tolerances	595:612	Within 8 wk of being fed a high-fat, high-sucrose diet, mice became obese, developed impaired insulin and glucose tolerances, and displayed noncompensatory insulin release, due, at least in part, to reduced expression of syntaxin-1A.
29957055	4	82	theme	anterior	767:774	arg1	chamber					776:782	the anterior chamber	763:782	the anterior chamber of the eye	763:793	Through reporter islets transplanted to the anterior chamber of the eye, we demonstrated a concomitant loss of functional β-cell mass.
29957055	3	83	theme	insulin	645:651	arg1	release					653:659	noncompensatory insulin release	629:659	noncompensatory insulin release	629:659	Within 8 wk of being fed a high-fat, high-sucrose diet, mice became obese, developed impaired insulin and glucose tolerances, and displayed noncompensatory insulin release, due, at least in part, to reduced expression of syntaxin-1A.
29957055	2	84	theme	diet	332:335	arg1	combinations					302:313	fed different combinations	288:313	fed different combinations of Western-style diet	288:335	Exemplified by diabetes-prone, male, C57B1/6J mice being fed different combinations of Western-style diet, we show that β-cell insulin resistance occurs early during T2DM progression and is due to a combination of lipotoxicity and increased β-cell workload.
29957055	7	85	dep	in	1241:1242	arg1	vivo					1244:1247	vivo	1244:1247	vivo	1244:1247	Employing the in vivo reporter islet-monitoring approach will allow researchers to define key times in the dynamics of reversible loss of functional β-cell mass and, thus, to investigate the underlying, molecular mechanisms involved in the progression toward T2DM manifestation.-Paschen, M., Moede, T., Valladolid-Acebes, I., Leibiger, B., Moruzzi, N., Jacob, S., García-Prieto, C. F., Brismar, K., Leibiger, I. B., Berggren, P.-O.
29675216	0	0	theme	improved	78:85	arg1	activity					101:108	improved antimicrobial activity	78:108	improved antimicrobial activity	78:108	Total chemical synthesis of glycocin F and analogues: S-glycosylation confers improved antimicrobial activity.
29675216	6	1	theme	biologically-inspired	1263:1283	arg1	route					1285:1289	a biologically-inspired route	1261:1289	a biologically-inspired route towards more active bacteriocins	1261:1322	Replacing the S-linked GlcNAc at residue 43 with an O-linked GlcNAc decreased the antibacterial activity, while replacing O-linked GlcNAc at position 18 with an S-linked GlcNAc increased the bioactivity suggesting that the S-glycosidic linkage may offer a biologically-inspired route towards more active bacteriocins.
29675216	1	2	theme	unique	134:139	arg1	F					120:120	Glycocin F	111:120	Glycocin F (GccF)	111:127	Glycocin F (GccF) is a unique diglycosylated bacteriocin peptide that possesses potent and reversible bacteriostatic activity against a range of Gram-positive bacteria.
29675216	1	2	theme	unique	134:139	arg1	peptide					168:174	a unique diglycosylated bacteriocin peptide	132:174	a unique diglycosylated bacteriocin peptide that possesses potent and reversible bacteriostatic activity against a range of Gram-positive bacteria	132:277	Glycocin F (GccF) is a unique diglycosylated bacteriocin peptide that possesses potent and reversible bacteriostatic activity against a range of Gram-positive bacteria.
29675216	5	3	theme	glycosidic	960:969	arg1	linkage					971:977	glycosidic linkage	960:977	glycosidic linkage	960:977	A second-generation synthetic strategy was used to prepare 2 site selective 'glyco-mutants' containing either two S-linked or two O-linked GlcNAc moieties; these mutants were used to probe the contribution of each type of glycosidic linkage to bacteriostatic activity.
29675216	6	4	theme	O-linked	1059:1066	arg1	GlcNAc					1068:1073	an O-linked GlcNAc	1056:1073	an O-linked GlcNAc	1056:1073	Replacing the S-linked GlcNAc at residue 43 with an O-linked GlcNAc decreased the antibacterial activity, while replacing O-linked GlcNAc at position 18 with an S-linked GlcNAc increased the bioactivity suggesting that the S-glycosidic linkage may offer a biologically-inspired route towards more active bacteriocins.
29675216	5	5	used	used	781:784	arg2	strategy					768:775	A second-generation synthetic strategy	738:775	A second-generation synthetic strategy	738:775	A second-generation synthetic strategy was used to prepare 2 site selective 'glyco-mutants' containing either two S-linked or two O-linked GlcNAc moieties; these mutants were used to probe the contribution of each type of glycosidic linkage to bacteriostatic activity.
29675216	1	6	theme	diglycosylated	141:154	arg1	F					120:120	Glycocin F	111:120	Glycocin F (GccF)	111:127	Glycocin F (GccF) is a unique diglycosylated bacteriocin peptide that possesses potent and reversible bacteriostatic activity against a range of Gram-positive bacteria.
29675216	1	6	theme	diglycosylated	141:154	arg1	peptide					168:174	a unique diglycosylated bacteriocin peptide	132:174	a unique diglycosylated bacteriocin peptide that possesses potent and reversible bacteriostatic activity against a range of Gram-positive bacteria	132:277	Glycocin F (GccF) is a unique diglycosylated bacteriocin peptide that possesses potent and reversible bacteriostatic activity against a range of Gram-positive bacteria.
29675216	1	7	theme	Gram-positive	256:268	arg1	bacteria					270:277	Gram-positive bacteria	256:277	Gram-positive bacteria	256:277	Glycocin F (GccF) is a unique diglycosylated bacteriocin peptide that possesses potent and reversible bacteriostatic activity against a range of Gram-positive bacteria.
29675216	6	8	from	residue	1040:1046	arg1	GlcNAc					1030:1035	the S-linked GlcNAc	1017:1035	the S-linked GlcNAc at residue 43	1017:1049	Replacing the S-linked GlcNAc at residue 43 with an O-linked GlcNAc decreased the antibacterial activity, while replacing O-linked GlcNAc at position 18 with an S-linked GlcNAc increased the bioactivity suggesting that the S-glycosidic linkage may offer a biologically-inspired route towards more active bacteriocins.
29675216	2	9	theme	rare	290:293	arg1	GccF					280:283	GccF	280:283	GccF	280:283	GccF is a rare example of a 'glycoactive' bacteriocin, with both the O-linked N-acetylglucosamine (GlcNAc) and the unusual S-linked GlcNAc moiety important for antibacterial activity.
29675216	2	9	theme	rare	290:293	arg1	example					295:301	a rare example	288:301	a rare example of a 'glycoactive' bacteriocin	288:332	GccF is a rare example of a 'glycoactive' bacteriocin, with both the O-linked N-acetylglucosamine (GlcNAc) and the unusual S-linked GlcNAc moiety important for antibacterial activity.
29675216	1	10	theme	bacteria	270:277	arg1	range					247:251	a range	245:251	a range of Gram-positive bacteria	245:277	Glycocin F (GccF) is a unique diglycosylated bacteriocin peptide that possesses potent and reversible bacteriostatic activity against a range of Gram-positive bacteria.
29675216	0	11	theme	antimicrobial	87:99	arg1	activity					101:108	improved antimicrobial activity	78:108	improved antimicrobial activity	78:108	Total chemical synthesis of glycocin F and analogues: S-glycosylation confers improved antimicrobial activity.
29675216	5	12	theme	linkage	971:977	arg1	type					952:955	each type	947:955	each type of glycosidic linkage	947:977	A second-generation synthetic strategy was used to prepare 2 site selective 'glyco-mutants' containing either two S-linked or two O-linked GlcNAc moieties; these mutants were used to probe the contribution of each type of glycosidic linkage to bacteriostatic activity.
29675216	6	13	theme	active	1304:1309	arg1	bacteriocins					1311:1322	more active bacteriocins	1299:1322	more active bacteriocins	1299:1322	Replacing the S-linked GlcNAc at residue 43 with an O-linked GlcNAc decreased the antibacterial activity, while replacing O-linked GlcNAc at position 18 with an S-linked GlcNAc increased the bioactivity suggesting that the S-glycosidic linkage may offer a biologically-inspired route towards more active bacteriocins.
29675216	3	14	theme	chemical	532:539	arg1	strategy					550:557	a native chemical ligation strategy	523:557	a native chemical ligation strategy	523:557	In this report, glycocin F was successfully prepared using a native chemical ligation strategy and folded into its native structure.
29675216	5	15	theme	O-linked	868:875	arg1	moieties					884:891	two O-linked GlcNAc moieties	864:891	two O-linked GlcNAc moieties	864:891	A second-generation synthetic strategy was used to prepare 2 site selective 'glyco-mutants' containing either two S-linked or two O-linked GlcNAc moieties; these mutants were used to probe the contribution of each type of glycosidic linkage to bacteriostatic activity.
29675216	5	16	theme	synthetic	758:766	arg1	strategy					768:775	A second-generation synthetic strategy	738:775	A second-generation synthetic strategy	738:775	A second-generation synthetic strategy was used to prepare 2 site selective 'glyco-mutants' containing either two S-linked or two O-linked GlcNAc moieties; these mutants were used to probe the contribution of each type of glycosidic linkage to bacteriostatic activity.
29675216	6	17	theme	antibacterial	1089:1101	arg1	activity					1103:1110	the antibacterial activity	1085:1110	the antibacterial activity	1085:1110	Replacing the S-linked GlcNAc at residue 43 with an O-linked GlcNAc decreased the antibacterial activity, while replacing O-linked GlcNAc at position 18 with an S-linked GlcNAc increased the bioactivity suggesting that the S-glycosidic linkage may offer a biologically-inspired route towards more active bacteriocins.
29675216	1	18	theme	bacteriocin	156:166	arg1	F					120:120	Glycocin F	111:120	Glycocin F (GccF)	111:127	Glycocin F (GccF) is a unique diglycosylated bacteriocin peptide that possesses potent and reversible bacteriostatic activity against a range of Gram-positive bacteria.
29675216	1	18	theme	bacteriocin	156:166	arg1	peptide					168:174	a unique diglycosylated bacteriocin peptide	132:174	a unique diglycosylated bacteriocin peptide that possesses potent and reversible bacteriostatic activity against a range of Gram-positive bacteria	132:277	Glycocin F (GccF) is a unique diglycosylated bacteriocin peptide that possesses potent and reversible bacteriostatic activity against a range of Gram-positive bacteria.
29675216	2	19	link	O-linked	349:356	arg1	GlcNAc					379:384	GlcNAc	379:384	GlcNAc	379:384	GccF is a rare example of a 'glycoactive' bacteriocin, with both the O-linked N-acetylglucosamine (GlcNAc) and the unusual S-linked GlcNAc moiety important for antibacterial activity.
29675216	2	19	link	O-linked	349:356	arg1	N-acetylglucosamine					358:376	O-linked N-acetylglucosamine	349:376	the O-linked N-acetylglucosamine (GlcNAc)	345:385	GccF is a rare example of a 'glycoactive' bacteriocin, with both the O-linked N-acetylglucosamine (GlcNAc) and the unusual S-linked GlcNAc moiety important for antibacterial activity.
29675216	2	20	theme	important	426:434	arg1	moiety					419:424	the unusual S-linked GlcNAc moiety	391:424	the unusual S-linked GlcNAc moiety important for antibacterial activity	391:461	GccF is a rare example of a 'glycoactive' bacteriocin, with both the O-linked N-acetylglucosamine (GlcNAc) and the unusual S-linked GlcNAc moiety important for antibacterial activity.
29675216	5	21	theme	GlcNAc	877:882	arg1	moieties					884:891	two O-linked GlcNAc moieties	864:891	two O-linked GlcNAc moieties	864:891	A second-generation synthetic strategy was used to prepare 2 site selective 'glyco-mutants' containing either two S-linked or two O-linked GlcNAc moieties; these mutants were used to probe the contribution of each type of glycosidic linkage to bacteriostatic activity.
29675216	0	22	theme	chemical	6:13	arg1	synthesis					15:23	Total chemical synthesis	0:23	Total chemical synthesis of glycocin F and analogues	0:51	Total chemical synthesis of glycocin F and analogues: S-glycosylation confers improved antimicrobial activity.
29675216	5	23	link	O-linked	868:875	arg1	moieties					884:891	two O-linked GlcNAc moieties	864:891	two O-linked GlcNAc moieties	864:891	A second-generation synthetic strategy was used to prepare 2 site selective 'glyco-mutants' containing either two S-linked or two O-linked GlcNAc moieties; these mutants were used to probe the contribution of each type of glycosidic linkage to bacteriostatic activity.
29675216	6	24	theme	S-linked	1168:1175	arg1	GlcNAc					1177:1182	an S-linked GlcNAc	1165:1182	an S-linked GlcNAc	1165:1182	Replacing the S-linked GlcNAc at residue 43 with an O-linked GlcNAc decreased the antibacterial activity, while replacing O-linked GlcNAc at position 18 with an S-linked GlcNAc increased the bioactivity suggesting that the S-glycosidic linkage may offer a biologically-inspired route towards more active bacteriocins.
29675216	0	25	theme	Total	0:4	arg1	synthesis					15:23	Total chemical synthesis	0:23	Total chemical synthesis of glycocin F and analogues	0:51	Total chemical synthesis of glycocin F and analogues: S-glycosylation confers improved antimicrobial activity.
29675216	5	26	dep	site	799:802	arg1	glyco-mutants					815:827	glyco-mutants	815:827	glyco-mutants	815:827	A second-generation synthetic strategy was used to prepare 2 site selective 'glyco-mutants' containing either two S-linked or two O-linked GlcNAc moieties; these mutants were used to probe the contribution of each type of glycosidic linkage to bacteriostatic activity.
29675216	6	27	link	O-linked	1129:1136	arg1	GlcNAc					1138:1143	O-linked GlcNAc	1129:1143	O-linked GlcNAc	1129:1143	Replacing the S-linked GlcNAc at residue 43 with an O-linked GlcNAc decreased the antibacterial activity, while replacing O-linked GlcNAc at position 18 with an S-linked GlcNAc increased the bioactivity suggesting that the S-glycosidic linkage may offer a biologically-inspired route towards more active bacteriocins.
29675216	3	28	theme	ligation	541:548	arg1	strategy					550:557	a native chemical ligation strategy	523:557	a native chemical ligation strategy	523:557	In this report, glycocin F was successfully prepared using a native chemical ligation strategy and folded into its native structure.
29675216	5	29	used	used	913:916	arg2	mutants					900:906	these mutants	894:906	these mutants	894:906	A second-generation synthetic strategy was used to prepare 2 site selective 'glyco-mutants' containing either two S-linked or two O-linked GlcNAc moieties; these mutants were used to probe the contribution of each type of glycosidic linkage to bacteriostatic activity.
29675216	1	30	theme	potent	191:196	arg1	activity					228:235	potent and reversible bacteriostatic activity	191:235	potent and reversible bacteriostatic activity	191:235	Glycocin F (GccF) is a unique diglycosylated bacteriocin peptide that possesses potent and reversible bacteriostatic activity against a range of Gram-positive bacteria.
29675216	2	31	theme	GlcNAc	412:417	arg1	moiety					419:424	the unusual S-linked GlcNAc moiety	391:424	the unusual S-linked GlcNAc moiety important for antibacterial activity	391:461	GccF is a rare example of a 'glycoactive' bacteriocin, with both the O-linked N-acetylglucosamine (GlcNAc) and the unusual S-linked GlcNAc moiety important for antibacterial activity.
29675216	1	32	contain	possesses	181:189	arg2	activity					228:235	potent and reversible bacteriostatic activity	191:235	potent and reversible bacteriostatic activity	191:235	Glycocin F (GccF) is a unique diglycosylated bacteriocin peptide that possesses potent and reversible bacteriostatic activity against a range of Gram-positive bacteria.
29675216	1	32	contain	possesses	181:189	arg1	F					120:120	Glycocin F	111:120	Glycocin F (GccF)	111:127	Glycocin F (GccF) is a unique diglycosylated bacteriocin peptide that possesses potent and reversible bacteriostatic activity against a range of Gram-positive bacteria.
29675216	1	32	contain	possesses	181:189	arg1	peptide					168:174	a unique diglycosylated bacteriocin peptide	132:174	a unique diglycosylated bacteriocin peptide that possesses potent and reversible bacteriostatic activity against a range of Gram-positive bacteria	132:277	Glycocin F (GccF) is a unique diglycosylated bacteriocin peptide that possesses potent and reversible bacteriostatic activity against a range of Gram-positive bacteria.
29675216	1	33	theme	Glycocin	111:118	arg1	GccF					123:126	GccF	123:126	GccF	123:126	Glycocin F (GccF) is a unique diglycosylated bacteriocin peptide that possesses potent and reversible bacteriostatic activity against a range of Gram-positive bacteria.
29675216	1	33	theme	Glycocin	111:118	arg1	F					120:120	Glycocin F	111:120	Glycocin F (GccF)	111:127	Glycocin F (GccF) is a unique diglycosylated bacteriocin peptide that possesses potent and reversible bacteriostatic activity against a range of Gram-positive bacteria.
29675216	1	33	theme	Glycocin	111:118	arg1	peptide					168:174	a unique diglycosylated bacteriocin peptide	132:174	a unique diglycosylated bacteriocin peptide that possesses potent and reversible bacteriostatic activity against a range of Gram-positive bacteria	132:277	Glycocin F (GccF) is a unique diglycosylated bacteriocin peptide that possesses potent and reversible bacteriostatic activity against a range of Gram-positive bacteria.
29675216	6	34	link	S-linked	1021:1028	arg1	GlcNAc					1030:1035	the S-linked GlcNAc	1017:1035	the S-linked GlcNAc at residue 43	1017:1049	Replacing the S-linked GlcNAc at residue 43 with an O-linked GlcNAc decreased the antibacterial activity, while replacing O-linked GlcNAc at position 18 with an S-linked GlcNAc increased the bioactivity suggesting that the S-glycosidic linkage may offer a biologically-inspired route towards more active bacteriocins.
29675216	2	35	theme	unusual	395:401	arg1	moiety					419:424	the unusual S-linked GlcNAc moiety	391:424	the unusual S-linked GlcNAc moiety important for antibacterial activity	391:461	GccF is a rare example of a 'glycoactive' bacteriocin, with both the O-linked N-acetylglucosamine (GlcNAc) and the unusual S-linked GlcNAc moiety important for antibacterial activity.
29675216	0	36	theme	F	37:37	arg1	synthesis					15:23	Total chemical synthesis	0:23	Total chemical synthesis of glycocin F and analogues	0:51	Total chemical synthesis of glycocin F and analogues: S-glycosylation confers improved antimicrobial activity.
29675216	3	37	theme	glycocin	480:487	arg1	F					489:489	glycocin F	480:489	glycocin F	480:489	In this report, glycocin F was successfully prepared using a native chemical ligation strategy and folded into its native structure.
29675216	5	38	contain	containing	830:839	arg2	moieties					884:891	two O-linked GlcNAc moieties	864:891	two O-linked GlcNAc moieties	864:891	A second-generation synthetic strategy was used to prepare 2 site selective 'glyco-mutants' containing either two S-linked or two O-linked GlcNAc moieties; these mutants were used to probe the contribution of each type of glycosidic linkage to bacteriostatic activity.
29675216	5	38	contain	containing	830:839	arg1	site					799:802	2 site selective	797:812	2 site selective 'glyco-mutants' containing either two S-linked or two O-linked GlcNAc moieties	797:891	A second-generation synthetic strategy was used to prepare 2 site selective 'glyco-mutants' containing either two S-linked or two O-linked GlcNAc moieties; these mutants were used to probe the contribution of each type of glycosidic linkage to bacteriostatic activity.
29675216	5	38	contain	containing	830:839	arg2	S-linked					852:859	S-linked	852:859	S-linked	852:859	A second-generation synthetic strategy was used to prepare 2 site selective 'glyco-mutants' containing either two S-linked or two O-linked GlcNAc moieties; these mutants were used to probe the contribution of each type of glycosidic linkage to bacteriostatic activity.
29675216	3	39	theme	native	579:584	arg1	structure					586:594	its native structure	575:594	its native structure	575:594	In this report, glycocin F was successfully prepared using a native chemical ligation strategy and folded into its native structure.
29675216	1	40	theme	reversible	202:211	arg1	activity					228:235	potent and reversible bacteriostatic activity	191:235	potent and reversible bacteriostatic activity	191:235	Glycocin F (GccF) is a unique diglycosylated bacteriocin peptide that possesses potent and reversible bacteriostatic activity against a range of Gram-positive bacteria.
29675216	0	41	theme	glycocin	28:35	arg1	F					37:37	glycocin F	28:37	glycocin F	28:37	Total chemical synthesis of glycocin F and analogues: S-glycosylation confers improved antimicrobial activity.
29675216	2	42	with	example	295:301	arg1	GlcNAc					379:384	GlcNAc	379:384	GlcNAc	379:384	GccF is a rare example of a 'glycoactive' bacteriocin, with both the O-linked N-acetylglucosamine (GlcNAc) and the unusual S-linked GlcNAc moiety important for antibacterial activity.
29675216	2	42	with	example	295:301	arg1	N-acetylglucosamine					358:376	O-linked N-acetylglucosamine	349:376	the O-linked N-acetylglucosamine (GlcNAc)	345:385	GccF is a rare example of a 'glycoactive' bacteriocin, with both the O-linked N-acetylglucosamine (GlcNAc) and the unusual S-linked GlcNAc moiety important for antibacterial activity.
29675216	2	42	with	example	295:301	arg1	moiety					419:424	the unusual S-linked GlcNAc moiety	391:424	the unusual S-linked GlcNAc moiety important for antibacterial activity	391:461	GccF is a rare example of a 'glycoactive' bacteriocin, with both the O-linked N-acetylglucosamine (GlcNAc) and the unusual S-linked GlcNAc moiety important for antibacterial activity.
29675216	6	43	theme	S-linked	1021:1028	arg1	GlcNAc					1030:1035	the S-linked GlcNAc	1017:1035	the S-linked GlcNAc at residue 43	1017:1049	Replacing the S-linked GlcNAc at residue 43 with an O-linked GlcNAc decreased the antibacterial activity, while replacing O-linked GlcNAc at position 18 with an S-linked GlcNAc increased the bioactivity suggesting that the S-glycosidic linkage may offer a biologically-inspired route towards more active bacteriocins.
29675216	1	44	theme	bacteriostatic	213:226	arg1	activity					228:235	potent and reversible bacteriostatic activity	191:235	potent and reversible bacteriostatic activity	191:235	Glycocin F (GccF) is a unique diglycosylated bacteriocin peptide that possesses potent and reversible bacteriostatic activity against a range of Gram-positive bacteria.
29675216	0	45	theme	analogues	43:51	arg1	synthesis					15:23	Total chemical synthesis	0:23	Total chemical synthesis of glycocin F and analogues	0:51	Total chemical synthesis of glycocin F and analogues: S-glycosylation confers improved antimicrobial activity.
29675216	6	46	link	O-linked	1059:1066	arg1	GlcNAc					1068:1073	an O-linked GlcNAc	1056:1073	an O-linked GlcNAc	1056:1073	Replacing the S-linked GlcNAc at residue 43 with an O-linked GlcNAc decreased the antibacterial activity, while replacing O-linked GlcNAc at position 18 with an S-linked GlcNAc increased the bioactivity suggesting that the S-glycosidic linkage may offer a biologically-inspired route towards more active bacteriocins.
29675216	4	47	theme	Lactobacillus	713:725	arg1	plantarum					727:735	Lactobacillus plantarum	713:735	Lactobacillus plantarum	713:735	The chemically synthesised glycocin appeared to be slightly more active than the recombinant material produced from Lactobacillus plantarum.
29675216	2	48	theme	antibacterial	440:452	arg1	activity					454:461	antibacterial activity	440:461	antibacterial activity	440:461	GccF is a rare example of a 'glycoactive' bacteriocin, with both the O-linked N-acetylglucosamine (GlcNAc) and the unusual S-linked GlcNAc moiety important for antibacterial activity.
29675216	2	49	theme	bacteriocin	322:332	arg1	GccF					280:283	GccF	280:283	GccF	280:283	GccF is a rare example of a 'glycoactive' bacteriocin, with both the O-linked N-acetylglucosamine (GlcNAc) and the unusual S-linked GlcNAc moiety important for antibacterial activity.
29675216	2	49	theme	bacteriocin	322:332	arg1	example					295:301	a rare example	288:301	a rare example of a 'glycoactive' bacteriocin	288:332	GccF is a rare example of a 'glycoactive' bacteriocin, with both the O-linked N-acetylglucosamine (GlcNAc) and the unusual S-linked GlcNAc moiety important for antibacterial activity.
29675216	1	50	gly	diglycosylated	141:154	arg1	F					120:120	Glycocin F	111:120	Glycocin F (GccF)	111:127	Glycocin F (GccF) is a unique diglycosylated bacteriocin peptide that possesses potent and reversible bacteriostatic activity against a range of Gram-positive bacteria.
29675216	1	50	gly	diglycosylated	141:154	arg1	peptide					168:174	a unique diglycosylated bacteriocin peptide	132:174	a unique diglycosylated bacteriocin peptide that possesses potent and reversible bacteriostatic activity against a range of Gram-positive bacteria	132:277	Glycocin F (GccF) is a unique diglycosylated bacteriocin peptide that possesses potent and reversible bacteriostatic activity against a range of Gram-positive bacteria.
29675216	6	51	theme	S-glycosidic	1230:1241	arg1	linkage					1243:1249	the S-glycosidic linkage	1226:1249	the S-glycosidic linkage	1226:1249	Replacing the S-linked GlcNAc at residue 43 with an O-linked GlcNAc decreased the antibacterial activity, while replacing O-linked GlcNAc at position 18 with an S-linked GlcNAc increased the bioactivity suggesting that the S-glycosidic linkage may offer a biologically-inspired route towards more active bacteriocins.
29675216	5	52	theme	type	952:955	arg1	contribution					931:942	the contribution	927:942	the contribution of each type of glycosidic linkage to bacteriostatic activity	927:1004	A second-generation synthetic strategy was used to prepare 2 site selective 'glyco-mutants' containing either two S-linked or two O-linked GlcNAc moieties; these mutants were used to probe the contribution of each type of glycosidic linkage to bacteriostatic activity.
29675216	5	53	theme	bacteriostatic	982:995	arg1	activity					997:1004	bacteriostatic activity	982:1004	bacteriostatic activity	982:1004	A second-generation synthetic strategy was used to prepare 2 site selective 'glyco-mutants' containing either two S-linked or two O-linked GlcNAc moieties; these mutants were used to probe the contribution of each type of glycosidic linkage to bacteriostatic activity.
29675216	2	54	link	S-linked	403:410	arg1	moiety					419:424	the unusual S-linked GlcNAc moiety	391:424	the unusual S-linked GlcNAc moiety important for antibacterial activity	391:461	GccF is a rare example of a 'glycoactive' bacteriocin, with both the O-linked N-acetylglucosamine (GlcNAc) and the unusual S-linked GlcNAc moiety important for antibacterial activity.
29675216	3	55	theme	native	525:530	arg1	strategy					550:557	a native chemical ligation strategy	523:557	a native chemical ligation strategy	523:557	In this report, glycocin F was successfully prepared using a native chemical ligation strategy and folded into its native structure.
29675216	2	56	theme	glycoactive	309:319	arg1	bacteriocin					322:332	a 'glycoactive' bacteriocin	306:332	a 'glycoactive' bacteriocin	306:332	GccF is a rare example of a 'glycoactive' bacteriocin, with both the O-linked N-acetylglucosamine (GlcNAc) and the unusual S-linked GlcNAc moiety important for antibacterial activity.
29675216	6	57	link	S-linked	1168:1175	arg1	GlcNAc					1177:1182	an S-linked GlcNAc	1165:1182	an S-linked GlcNAc	1165:1182	Replacing the S-linked GlcNAc at residue 43 with an O-linked GlcNAc decreased the antibacterial activity, while replacing O-linked GlcNAc at position 18 with an S-linked GlcNAc increased the bioactivity suggesting that the S-glycosidic linkage may offer a biologically-inspired route towards more active bacteriocins.
29675216	2	58	theme	S-linked	403:410	arg1	moiety					419:424	the unusual S-linked GlcNAc moiety	391:424	the unusual S-linked GlcNAc moiety important for antibacterial activity	391:461	GccF is a rare example of a 'glycoactive' bacteriocin, with both the O-linked N-acetylglucosamine (GlcNAc) and the unusual S-linked GlcNAc moiety important for antibacterial activity.
29675216	2	59	theme	O-linked	349:356	arg1	GlcNAc					379:384	GlcNAc	379:384	GlcNAc	379:384	GccF is a rare example of a 'glycoactive' bacteriocin, with both the O-linked N-acetylglucosamine (GlcNAc) and the unusual S-linked GlcNAc moiety important for antibacterial activity.
29675216	2	59	theme	O-linked	349:356	arg1	N-acetylglucosamine					358:376	O-linked N-acetylglucosamine	349:376	the O-linked N-acetylglucosamine (GlcNAc)	345:385	GccF is a rare example of a 'glycoactive' bacteriocin, with both the O-linked N-acetylglucosamine (GlcNAc) and the unusual S-linked GlcNAc moiety important for antibacterial activity.
29675216	4	60	theme	synthesised	612:622	arg1	glycocin					624:631	The chemically synthesised glycocin	597:631	The chemically synthesised glycocin	597:631	The chemically synthesised glycocin appeared to be slightly more active than the recombinant material produced from Lactobacillus plantarum.
29675216	4	60	theme	synthesised	612:622	arg1	active					662:667	active	662:667	active	662:667	The chemically synthesised glycocin appeared to be slightly more active than the recombinant material produced from Lactobacillus plantarum.
29675216	5	61	theme	selective	804:812	arg1	site					799:802	2 site selective	797:812	2 site selective 'glyco-mutants' containing either two S-linked or two O-linked GlcNAc moieties	797:891	A second-generation synthetic strategy was used to prepare 2 site selective 'glyco-mutants' containing either two S-linked or two O-linked GlcNAc moieties; these mutants were used to probe the contribution of each type of glycosidic linkage to bacteriostatic activity.
29675216	6	62	theme	O-linked	1129:1136	arg1	GlcNAc					1138:1143	O-linked GlcNAc	1129:1143	O-linked GlcNAc	1129:1143	Replacing the S-linked GlcNAc at residue 43 with an O-linked GlcNAc decreased the antibacterial activity, while replacing O-linked GlcNAc at position 18 with an S-linked GlcNAc increased the bioactivity suggesting that the S-glycosidic linkage may offer a biologically-inspired route towards more active bacteriocins.
29675216	5	63	theme	second-generation	740:756	arg1	strategy					768:775	A second-generation synthetic strategy	738:775	A second-generation synthetic strategy	738:775	A second-generation synthetic strategy was used to prepare 2 site selective 'glyco-mutants' containing either two S-linked or two O-linked GlcNAc moieties; these mutants were used to probe the contribution of each type of glycosidic linkage to bacteriostatic activity.
29948824	6	0	theme	sequence	731:738	arg1	similarity					707:716	The similarity	703:716	The similarity of rpoB gene sequence between strain YT-3T and B. drentensis JCM 21707T, B. ginsengisoli JCM 17335T and B. fumarioli JCM 21708T	703:844	The similarity of rpoB gene sequence between strain YT-3T and B. drentensis JCM 21707T, B. ginsengisoli JCM 17335T and B. fumarioli JCM 21708T was 80.4%, 81.5%, and 82.1%, respectively.
29948824	6	0	theme	sequence	731:738	arg1	%					854:854	80.4%	850:854	80.4%	850:854	The similarity of rpoB gene sequence between strain YT-3T and B. drentensis JCM 21707T, B. ginsengisoli JCM 17335T and B. fumarioli JCM 21708T was 80.4%, 81.5%, and 82.1%, respectively.
29948824	3	1	dep	Gram-positive	226:238	arg1	rod-shaped					241:250	rod-shaped	241:250	rod-shaped	241:250	Cells of strain YT-3T were Gram-positive, rod-shaped, facultative anaerobic, catalase, and oxidase positive.
29948824	3	1	dep	Gram-positive	226:238	arg1	facultative					253:263	facultative	253:263	facultative	253:263	Cells of strain YT-3T were Gram-positive, rod-shaped, facultative anaerobic, catalase, and oxidase positive.
29948824	7	2	theme	genomic	893:899	arg1	%					931:931	44.9 mol%	923:931	44.9 mol%	923:931	The genomic DNA G + C content was 44.9 mol%.
29948824	7	2	theme	genomic	893:899	arg1	G					905:905	The genomic DNA G + C content	889:917	G	905:905	The genomic DNA G + C content was 44.9 mol%.
29948824	6	3	theme	rpoB	721:724	arg1	sequence					731:738	rpoB gene sequence	721:738	rpoB gene sequence	721:738	The similarity of rpoB gene sequence between strain YT-3T and B. drentensis JCM 21707T, B. ginsengisoli JCM 17335T and B. fumarioli JCM 21708T was 80.4%, 81.5%, and 82.1%, respectively.
29948824	5	4	theme	highest	549:555	arg1	similarity					557:566	the highest similarity	545:566	the highest similarity to that of Bacillus drentensis JCM 21707T	545:608	Analysis of the 16S rRNA gene sequence showed that strain YT-3T was affiliated to the genus Bacillus and displayed the highest similarity to that of Bacillus drentensis JCM 21707T (98.3%), followed by B. ginsengisoli JCM 17335T (97.8%) and B. fumarioli JCM 21708T (97.0%).
29948824	12	5	theme	novel	1612:1616	arg1	species					1618:1624	a novel species	1610:1624	a novel species	1610:1624	On the basis of physiological, genetic and biochemical data, strain YT-3T represented a novel species of the genus Bacillus, for which the name Bacillus ferrooxidans sp.
29948824	14	6	theme	CCTCC	1754:1758	arg1	2017049T					1763:1770	= KCTC 33875T = CCTCC AB 2017049T	1738:1770	= KCTC 33875T = CCTCC AB 2017049T	1738:1770	The type strain is YT-3T (= KCTC 33875T = CCTCC AB 2017049T).
29948824	14	6	theme	CCTCC	1754:1758	arg1	YT-3T					1731:1735	YT-3T	1731:1735	YT-3T (= KCTC 33875T = CCTCC AB 2017049T)	1731:1771	The type strain is YT-3T (= KCTC 33875T = CCTCC AB 2017049T).
29948824	11	7	theme	hybridization	1345:1357	arg1	values					1359:1364	The DNA-DNA hybridization values	1333:1364	The DNA-DNA hybridization values between isolate YT-3T and B. drentensis (JCM 21707T), B. ginsengisoli (JCM 17335T), and B. fumarioli (JCM 21708T)	1333:1478	The DNA-DNA hybridization values between isolate YT-3T and B. drentensis (JCM 21707T), B. ginsengisoli (JCM 17335T), and B. fumarioli (JCM 21708T) were 36.3%, 30.3%, and 25.3%, respectively.
29948824	11	7	theme	hybridization	1345:1357	arg1	%					1489:1489	36.3%	1485:1489	36.3%	1485:1489	The DNA-DNA hybridization values between isolate YT-3T and B. drentensis (JCM 21707T), B. ginsengisoli (JCM 17335T), and B. fumarioli (JCM 21708T) were 36.3%, 30.3%, and 25.3%, respectively.
29948824	8	8	attach	present	1022:1028	arg2	acid					1013:1016	meso-diaminopimelic acid	993:1016	meso-diaminopimelic acid	993:1016	The predominant respiratory quinone was Menaquinone-7, and meso-diaminopimelic acid was present in the peptidoglycan layer of cell wall.
29948824	8	8	attach	present	1022:1028	arg1	layer					1051:1055	the peptidoglycan layer	1033:1055	the peptidoglycan layer of cell wall	1033:1068	The predominant respiratory quinone was Menaquinone-7, and meso-diaminopimelic acid was present in the peptidoglycan layer of cell wall.
29948824	7	9	theme	mol	928:930	arg1	%					931:931	44.9 mol%	923:931	44.9 mol%	923:931	The genomic DNA G + C content was 44.9 mol%.
29948824	7	9	theme	mol	928:930	arg1	G					905:905	The genomic DNA G + C content	889:917	G	905:905	The genomic DNA G + C content was 44.9 mol%.
29948824	9	10	theme	C15:0	1140:1144	arg1	iso					1146:1148	C15:0 iso	1140:1148	C15:0 iso (17.4%)	1140:1156	The major fatty acids were C15:0 anteiso (36.2%), C14:0 iso (19.6%), C15:0 iso (17.4%), and C16:0 iso (9.8%).
29948824	9	10	theme	C15:0	1140:1144	arg1	%					1155:1155	17.4%	1151:1155	17.4%	1151:1155	The major fatty acids were C15:0 anteiso (36.2%), C14:0 iso (19.6%), C15:0 iso (17.4%), and C16:0 iso (9.8%).
29948824	14	11	theme	33875T	1745:1750	arg1	2017049T					1763:1770	= KCTC 33875T = CCTCC AB 2017049T	1738:1770	= KCTC 33875T = CCTCC AB 2017049T	1738:1770	The type strain is YT-3T (= KCTC 33875T = CCTCC AB 2017049T).
29948824	14	11	theme	33875T	1745:1750	arg1	YT-3T					1731:1735	YT-3T	1731:1735	YT-3T (= KCTC 33875T = CCTCC AB 2017049T)	1731:1771	The type strain is YT-3T (= KCTC 33875T = CCTCC AB 2017049T).
29948824	12	12	theme	strain	1585:1590	arg1	YT-3T					1592:1596	strain YT-3T	1585:1596	strain YT-3T	1585:1596	On the basis of physiological, genetic and biochemical data, strain YT-3T represented a novel species of the genus Bacillus, for which the name Bacillus ferrooxidans sp.
29948824	4	13	theme	growth	320:325	arg1	temperature					327:337	The optimum growth temperature	308:337	The optimum growth temperature	308:337	The optimum growth temperature and pH were 30°C (ranged from 15 to 50°C) and 6.5-7.0 (ranged from 3 to 11), respectively.
29948824	4	13	theme	growth	320:325	arg1	30°C					351:354	30°C	351:354	30°C	351:354	The optimum growth temperature and pH were 30°C (ranged from 15 to 50°C) and 6.5-7.0 (ranged from 3 to 11), respectively.
29948824	14	14	theme	=	1738:1738	arg1	2017049T					1763:1770	= KCTC 33875T = CCTCC AB 2017049T	1738:1770	= KCTC 33875T = CCTCC AB 2017049T	1738:1770	The type strain is YT-3T (= KCTC 33875T = CCTCC AB 2017049T).
29948824	14	14	theme	=	1738:1738	arg1	YT-3T					1731:1735	YT-3T	1731:1735	YT-3T (= KCTC 33875T = CCTCC AB 2017049T)	1731:1771	The type strain is YT-3T (= KCTC 33875T = CCTCC AB 2017049T).
29948824	5	15	theme	drentensis	588:597	arg1	21707T					603:608	Bacillus drentensis JCM 21707T	579:608	Bacillus drentensis JCM 21707T	579:608	Analysis of the 16S rRNA gene sequence showed that strain YT-3T was affiliated to the genus Bacillus and displayed the highest similarity to that of Bacillus drentensis JCM 21707T (98.3%), followed by B. ginsengisoli JCM 17335T (97.8%) and B. fumarioli JCM 21708T (97.0%).
29948824	5	16	theme	fumarioli	673:681	arg1	21708T					687:692	B. fumarioli JCM 21708T	670:692	B. fumarioli JCM 21708T (97.0%)	670:700	Analysis of the 16S rRNA gene sequence showed that strain YT-3T was affiliated to the genus Bacillus and displayed the highest similarity to that of Bacillus drentensis JCM 21707T (98.3%), followed by B. ginsengisoli JCM 17335T (97.8%) and B. fumarioli JCM 21708T (97.0%).
29948824	5	16	theme	fumarioli	673:681	arg1	%					699:699	97.0%	695:699	97.0%	695:699	Analysis of the 16S rRNA gene sequence showed that strain YT-3T was affiliated to the genus Bacillus and displayed the highest similarity to that of Bacillus drentensis JCM 21707T (98.3%), followed by B. ginsengisoli JCM 17335T (97.8%) and B. fumarioli JCM 21708T (97.0%).
29948824	3	17	theme	strain	208:213	arg1	YT-3T					215:219	strain YT-3T	208:219	strain YT-3T	208:219	Cells of strain YT-3T were Gram-positive, rod-shaped, facultative anaerobic, catalase, and oxidase positive.
29948824	11	18	theme	JCM	1468:1470	arg1	fumarioli					1457:1465	B. drentensis (JCM 21707T), B. ginsengisoli (JCM 17335T), and B. fumarioli	1392:1465	B. drentensis (JCM 21707T), B. ginsengisoli (JCM 17335T), and B. fumarioli (JCM 21708T)	1392:1478	The DNA-DNA hybridization values between isolate YT-3T and B. drentensis (JCM 21707T), B. ginsengisoli (JCM 17335T), and B. fumarioli (JCM 21708T) were 36.3%, 30.3%, and 25.3%, respectively.
29948824	11	18	theme	JCM	1468:1470	arg1	21708T					1472:1477	JCM 21708T	1468:1477	JCM 21708T	1468:1477	The DNA-DNA hybridization values between isolate YT-3T and B. drentensis (JCM 21707T), B. ginsengisoli (JCM 17335T), and B. fumarioli (JCM 21708T) were 36.3%, 30.3%, and 25.3%, respectively.
29948824	5	19	theme	16S	446:448	arg1	rRNA					450:453	the 16S rRNA	442:453	the 16S rRNA gene sequence	442:467	Analysis of the 16S rRNA gene sequence showed that strain YT-3T was affiliated to the genus Bacillus and displayed the highest similarity to that of Bacillus drentensis JCM 21707T (98.3%), followed by B. ginsengisoli JCM 17335T (97.8%) and B. fumarioli JCM 21708T (97.0%).
29948824	9	20	theme	C16:0	1163:1167	arg1	iso					1169:1171	C16:0 iso	1163:1171	C16:0 iso (9.8%)	1163:1178	The major fatty acids were C15:0 anteiso (36.2%), C14:0 iso (19.6%), C15:0 iso (17.4%), and C16:0 iso (9.8%).
29948824	9	20	theme	C16:0	1163:1167	arg1	%					1177:1177	9.8%	1174:1177	9.8%	1174:1177	The major fatty acids were C15:0 anteiso (36.2%), C14:0 iso (19.6%), C15:0 iso (17.4%), and C16:0 iso (9.8%).
29948824	6	21	theme	strain	748:753	arg1	YT-3T					755:759	strain YT-3T	748:759	strain YT-3T	748:759	The similarity of rpoB gene sequence between strain YT-3T and B. drentensis JCM 21707T, B. ginsengisoli JCM 17335T and B. fumarioli JCM 21708T was 80.4%, 81.5%, and 82.1%, respectively.
29948824	12	22	theme	ferrooxidans	1677:1688	arg1	sp					1690:1691	Bacillus ferrooxidans sp	1668:1691	the name Bacillus ferrooxidans sp	1659:1691	On the basis of physiological, genetic and biochemical data, strain YT-3T represented a novel species of the genus Bacillus, for which the name Bacillus ferrooxidans sp.
29948824	11	23	theme	ginsengisoli	1423:1434	arg1	fumarioli					1457:1465	B. drentensis (JCM 21707T), B. ginsengisoli (JCM 17335T), and B. fumarioli	1392:1465	B. drentensis (JCM 21707T), B. ginsengisoli (JCM 17335T), and B. fumarioli (JCM 21708T)	1392:1478	The DNA-DNA hybridization values between isolate YT-3T and B. drentensis (JCM 21707T), B. ginsengisoli (JCM 17335T), and B. fumarioli (JCM 21708T) were 36.3%, 30.3%, and 25.3%, respectively.
29948824	11	23	theme	ginsengisoli	1423:1434	arg1	21708T					1472:1477	JCM 21708T	1468:1477	JCM 21708T	1468:1477	The DNA-DNA hybridization values between isolate YT-3T and B. drentensis (JCM 21707T), B. ginsengisoli (JCM 17335T), and B. fumarioli (JCM 21708T) were 36.3%, 30.3%, and 25.3%, respectively.
29948824	8	24	theme	cell	1060:1063	arg1	wall					1065:1068	cell wall	1060:1068	cell wall	1060:1068	The predominant respiratory quinone was Menaquinone-7, and meso-diaminopimelic acid was present in the peptidoglycan layer of cell wall.
29948824	8	25	from	present	1022:1028	arg1	layer					1051:1055	the peptidoglycan layer	1033:1055	the peptidoglycan layer of cell wall	1033:1068	The predominant respiratory quinone was Menaquinone-7, and meso-diaminopimelic acid was present in the peptidoglycan layer of cell wall.
29948824	14	26	theme	type	1716:1719	arg1	YT-3T					1731:1735	YT-3T	1731:1735	YT-3T (= KCTC 33875T = CCTCC AB 2017049T)	1731:1771	The type strain is YT-3T (= KCTC 33875T = CCTCC AB 2017049T).
29948824	14	26	theme	type	1716:1719	arg1	strain					1721:1726	The type strain	1712:1726	The type strain	1712:1726	The type strain is YT-3T (= KCTC 33875T = CCTCC AB 2017049T).
29948824	5	27	theme	B.	631:632	arg1	17335T					651:656	B. ginsengisoli JCM 17335T	631:656	B. ginsengisoli JCM 17335T (97.8%)	631:664	Analysis of the 16S rRNA gene sequence showed that strain YT-3T was affiliated to the genus Bacillus and displayed the highest similarity to that of Bacillus drentensis JCM 21707T (98.3%), followed by B. ginsengisoli JCM 17335T (97.8%) and B. fumarioli JCM 21708T (97.0%).
29948824	5	27	theme	B.	631:632	arg1	%					663:663	97.8%	659:663	97.8%	659:663	Analysis of the 16S rRNA gene sequence showed that strain YT-3T was affiliated to the genus Bacillus and displayed the highest similarity to that of Bacillus drentensis JCM 21707T (98.3%), followed by B. ginsengisoli JCM 17335T (97.8%) and B. fumarioli JCM 21708T (97.0%).
29948824	5	28	theme	strain	481:486	arg1	YT-3T					488:492	strain YT-3T	481:492	strain YT-3T	481:492	Analysis of the 16S rRNA gene sequence showed that strain YT-3T was affiliated to the genus Bacillus and displayed the highest similarity to that of Bacillus drentensis JCM 21707T (98.3%), followed by B. ginsengisoli JCM 17335T (97.8%) and B. fumarioli JCM 21708T (97.0%).
29948824	6	29	theme	JCM	807:809	arg1	17335T					811:816	B. ginsengisoli JCM 17335T	791:816	B. ginsengisoli JCM 17335T	791:816	The similarity of rpoB gene sequence between strain YT-3T and B. drentensis JCM 21707T, B. ginsengisoli JCM 17335T and B. fumarioli JCM 21708T was 80.4%, 81.5%, and 82.1%, respectively.
29948824	11	30	theme	JCM	1437:1439	arg1	ginsengisoli					1423:1434	ginsengisoli	1423:1434	ginsengisoli	1423:1434	The DNA-DNA hybridization values between isolate YT-3T and B. drentensis (JCM 21707T), B. ginsengisoli (JCM 17335T), and B. fumarioli (JCM 21708T) were 36.3%, 30.3%, and 25.3%, respectively.
29948824	11	30	theme	JCM	1437:1439	arg1	17335T					1441:1446	JCM 17335T	1437:1446	JCM 17335T	1437:1446	The DNA-DNA hybridization values between isolate YT-3T and B. drentensis (JCM 21707T), B. ginsengisoli (JCM 17335T), and B. fumarioli (JCM 21708T) were 36.3%, 30.3%, and 25.3%, respectively.
29948824	8	31	from	layer	1051:1055	arg1	present					1022:1028	present	1022:1028	present	1022:1028	The predominant respiratory quinone was Menaquinone-7, and meso-diaminopimelic acid was present in the peptidoglycan layer of cell wall.
29948824	6	32	theme	B.	791:792	arg1	17335T					811:816	B. ginsengisoli JCM 17335T	791:816	B. ginsengisoli JCM 17335T	791:816	The similarity of rpoB gene sequence between strain YT-3T and B. drentensis JCM 21707T, B. ginsengisoli JCM 17335T and B. fumarioli JCM 21708T was 80.4%, 81.5%, and 82.1%, respectively.
29948824	8	33	located	present	1022:1028	arg2	acid					1013:1016	meso-diaminopimelic acid	993:1016	meso-diaminopimelic acid	993:1016	The predominant respiratory quinone was Menaquinone-7, and meso-diaminopimelic acid was present in the peptidoglycan layer of cell wall.
29948824	8	33	located	present	1022:1028	arg1	layer					1051:1055	the peptidoglycan layer	1033:1055	the peptidoglycan layer of cell wall	1033:1068	The predominant respiratory quinone was Menaquinone-7, and meso-diaminopimelic acid was present in the peptidoglycan layer of cell wall.
29948824	5	34	theme	gene	455:458	arg1	sequence					460:467	the 16S rRNA gene sequence	442:467	the 16S rRNA gene sequence	442:467	Analysis of the 16S rRNA gene sequence showed that strain YT-3T was affiliated to the genus Bacillus and displayed the highest similarity to that of Bacillus drentensis JCM 21707T (98.3%), followed by B. ginsengisoli JCM 17335T (97.8%) and B. fumarioli JCM 21708T (97.0%).
29948824	1	35	theme	-oxidizing	43:52	arg1	nov.					26:29	nov.	26:29	nov.	26:29	nov., an iron(II)-oxidizing bacterium isolated from paddy soil.
29948824	1	35	theme	-oxidizing	43:52	arg1	bacterium					54:62	an iron(II)-oxidizing bacterium	32:62	an iron(II)-oxidizing bacterium	32:62	nov., an iron(II)-oxidizing bacterium isolated from paddy soil.
29948824	12	36	dep	data	1579:1582	arg1	the					1527:1529	the	1527:1529	the	1527:1529	On the basis of physiological, genetic and biochemical data, strain YT-3T represented a novel species of the genus Bacillus, for which the name Bacillus ferrooxidans sp.
29948824	12	36	dep	data	1579:1582	arg1	basis					1531:1535	basis	1531:1535	basis	1531:1535	On the basis of physiological, genetic and biochemical data, strain YT-3T represented a novel species of the genus Bacillus, for which the name Bacillus ferrooxidans sp.
29948824	0	37	theme	Bacillus	0:7	arg1	sp					22:23	Bacillus ferrooxidans sp	0:23	Bacillus ferrooxidans sp.	0:24	Bacillus ferrooxidans sp.
29948824	9	38	theme	fatty	1081:1085	arg1	iso					1127:1129	C15:0 anteiso (36.2%), C14:0 iso	1098:1129	C15:0 anteiso (36.2%), C14:0 iso (19.6%)	1098:1137	The major fatty acids were C15:0 anteiso (36.2%), C14:0 iso (19.6%), C15:0 iso (17.4%), and C16:0 iso (9.8%).
29948824	9	38	theme	fatty	1081:1085	arg1	acids					1087:1091	The major fatty acids	1071:1091	The major fatty acids	1071:1091	The major fatty acids were C15:0 anteiso (36.2%), C14:0 iso (19.6%), C15:0 iso (17.4%), and C16:0 iso (9.8%).
29948824	1	39	theme	paddy	78:82	arg1	soil					84:87	paddy soil	78:87	paddy soil	78:87	nov., an iron(II)-oxidizing bacterium isolated from paddy soil.
29948824	7	40	theme	C	909:909	arg1	content					911:917	The genomic DNA G + C content	889:917	content	911:917	The genomic DNA G + C content was 44.9 mol%.
29948824	9	41	theme	anteiso	1104:1110	arg1	iso					1127:1129	C15:0 anteiso (36.2%), C14:0 iso	1098:1129	C15:0 anteiso (36.2%), C14:0 iso (19.6%)	1098:1137	The major fatty acids were C15:0 anteiso (36.2%), C14:0 iso (19.6%), C15:0 iso (17.4%), and C16:0 iso (9.8%).
29948824	9	41	theme	anteiso	1104:1110	arg1	%					1136:1136	19.6%	1132:1136	19.6%	1132:1136	The major fatty acids were C15:0 anteiso (36.2%), C14:0 iso (19.6%), C15:0 iso (17.4%), and C16:0 iso (9.8%).
29948824	9	41	theme	anteiso	1104:1110	arg1	acids					1087:1091	The major fatty acids	1071:1091	The major fatty acids	1071:1091	The major fatty acids were C15:0 anteiso (36.2%), C14:0 iso (19.6%), C15:0 iso (17.4%), and C16:0 iso (9.8%).
29948824	5	42	theme	JCM	647:649	arg1	17335T					651:656	B. ginsengisoli JCM 17335T	631:656	B. ginsengisoli JCM 17335T (97.8%)	631:664	Analysis of the 16S rRNA gene sequence showed that strain YT-3T was affiliated to the genus Bacillus and displayed the highest similarity to that of Bacillus drentensis JCM 21707T (98.3%), followed by B. ginsengisoli JCM 17335T (97.8%) and B. fumarioli JCM 21708T (97.0%).
29948824	5	42	theme	JCM	647:649	arg1	%					663:663	97.8%	659:663	97.8%	659:663	Analysis of the 16S rRNA gene sequence showed that strain YT-3T was affiliated to the genus Bacillus and displayed the highest similarity to that of Bacillus drentensis JCM 21707T (98.3%), followed by B. ginsengisoli JCM 17335T (97.8%) and B. fumarioli JCM 21708T (97.0%).
29948824	6	43	theme	fumarioli	825:833	arg1	21708T					839:844	B. fumarioli JCM 21708T	822:844	B. fumarioli JCM 21708T	822:844	The similarity of rpoB gene sequence between strain YT-3T and B. drentensis JCM 21707T, B. ginsengisoli JCM 17335T and B. fumarioli JCM 21708T was 80.4%, 81.5%, and 82.1%, respectively.
29948824	8	44	theme	wall	1065:1068	arg1	layer					1051:1055	the peptidoglycan layer	1033:1055	the peptidoglycan layer of cell wall	1033:1068	The predominant respiratory quinone was Menaquinone-7, and meso-diaminopimelic acid was present in the peptidoglycan layer of cell wall.
29948824	11	45	theme	JCM	1407:1409	arg1	B.					1392:1393	B.	1392:1393	B.	1392:1393	The DNA-DNA hybridization values between isolate YT-3T and B. drentensis (JCM 21707T), B. ginsengisoli (JCM 17335T), and B. fumarioli (JCM 21708T) were 36.3%, 30.3%, and 25.3%, respectively.
29948824	11	45	theme	JCM	1407:1409	arg1	21707T					1411:1416	JCM 21707T	1407:1416	JCM 21707T	1407:1416	The DNA-DNA hybridization values between isolate YT-3T and B. drentensis (JCM 21707T), B. ginsengisoli (JCM 17335T), and B. fumarioli (JCM 21708T) were 36.3%, 30.3%, and 25.3%, respectively.
29948824	11	46	dep	B.	1392:1393	arg1	drentensis					1395:1404	B. drentensis	1392:1404	B. drentensis (JCM 21707T)	1392:1417	The DNA-DNA hybridization values between isolate YT-3T and B. drentensis (JCM 21707T), B. ginsengisoli (JCM 17335T), and B. fumarioli (JCM 21708T) were 36.3%, 30.3%, and 25.3%, respectively.
29948824	1	47	theme	iron	35:38	arg1	nov.					26:29	nov.	26:29	nov.	26:29	nov., an iron(II)-oxidizing bacterium isolated from paddy soil.
29948824	1	47	theme	iron	35:38	arg1	bacterium					54:62	an iron(II)-oxidizing bacterium	32:62	an iron(II)-oxidizing bacterium	32:62	nov., an iron(II)-oxidizing bacterium isolated from paddy soil.
29948824	10	48	theme	polar	1185:1189	arg1	profile					1197:1203	The polar lipid profile	1181:1203	The polar lipid profile	1181:1203	The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phospholipids, and ammoniac phospholipids.
29948824	11	49	theme	DNA-DNA	1337:1343	arg1	values					1359:1364	The DNA-DNA hybridization values	1333:1364	The DNA-DNA hybridization values between isolate YT-3T and B. drentensis (JCM 21707T), B. ginsengisoli (JCM 17335T), and B. fumarioli (JCM 21708T)	1333:1478	The DNA-DNA hybridization values between isolate YT-3T and B. drentensis (JCM 21707T), B. ginsengisoli (JCM 17335T), and B. fumarioli (JCM 21708T) were 36.3%, 30.3%, and 25.3%, respectively.
29948824	11	49	theme	DNA-DNA	1337:1343	arg1	%					1489:1489	36.3%	1485:1489	36.3%	1485:1489	The DNA-DNA hybridization values between isolate YT-3T and B. drentensis (JCM 21707T), B. ginsengisoli (JCM 17335T), and B. fumarioli (JCM 21708T) were 36.3%, 30.3%, and 25.3%, respectively.
29948824	6	50	theme	gene	726:729	arg1	sequence					731:738	rpoB gene sequence	721:738	rpoB gene sequence	721:738	The similarity of rpoB gene sequence between strain YT-3T and B. drentensis JCM 21707T, B. ginsengisoli JCM 17335T and B. fumarioli JCM 21708T was 80.4%, 81.5%, and 82.1%, respectively.
29948824	4	51	dep	50°C	375:378	arg1	to					372:373	to	372:373	to	372:373	The optimum growth temperature and pH were 30°C (ranged from 15 to 50°C) and 6.5-7.0 (ranged from 3 to 11), respectively.
29948824	14	52	theme	AB	1760:1761	arg1	2017049T					1763:1770	= KCTC 33875T = CCTCC AB 2017049T	1738:1770	= KCTC 33875T = CCTCC AB 2017049T	1738:1770	The type strain is YT-3T (= KCTC 33875T = CCTCC AB 2017049T).
29948824	14	52	theme	AB	1760:1761	arg1	YT-3T					1731:1735	YT-3T	1731:1735	YT-3T (= KCTC 33875T = CCTCC AB 2017049T)	1731:1771	The type strain is YT-3T (= KCTC 33875T = CCTCC AB 2017049T).
29948824	11	53	theme	isolate	1374:1380	arg1	YT-3T					1382:1386	isolate YT-3T	1374:1386	isolate YT-3T	1374:1386	The DNA-DNA hybridization values between isolate YT-3T and B. drentensis (JCM 21707T), B. ginsengisoli (JCM 17335T), and B. fumarioli (JCM 21708T) were 36.3%, 30.3%, and 25.3%, respectively.
29948824	2	54	from	soil	166:169	arg1	Jiangxi					183:189	Jiangxi	183:189	Jiangxi	183:189	An endospore-forming bacterium, designated YT-3T, was isolated from a paddy soil in Yingtan, Jiangxi, China.
29948824	2	54	from	soil	166:169	arg1	China					192:196	China	192:196	China	192:196	An endospore-forming bacterium, designated YT-3T, was isolated from a paddy soil in Yingtan, Jiangxi, China.
29948824	4	55	theme	optimum	312:318	arg1	temperature					327:337	The optimum growth temperature	308:337	The optimum growth temperature	308:337	The optimum growth temperature and pH were 30°C (ranged from 15 to 50°C) and 6.5-7.0 (ranged from 3 to 11), respectively.
29948824	4	55	theme	optimum	312:318	arg1	30°C					351:354	30°C	351:354	30°C	351:354	The optimum growth temperature and pH were 30°C (ranged from 15 to 50°C) and 6.5-7.0 (ranged from 3 to 11), respectively.
29948824	14	56	theme	=	1752:1752	arg1	2017049T					1763:1770	= KCTC 33875T = CCTCC AB 2017049T	1738:1770	= KCTC 33875T = CCTCC AB 2017049T	1738:1770	The type strain is YT-3T (= KCTC 33875T = CCTCC AB 2017049T).
29948824	14	56	theme	=	1752:1752	arg1	YT-3T					1731:1735	YT-3T	1731:1735	YT-3T (= KCTC 33875T = CCTCC AB 2017049T)	1731:1771	The type strain is YT-3T (= KCTC 33875T = CCTCC AB 2017049T).
29948824	12	57	theme	biochemical	1567:1577	arg1	data					1579:1582	physiological, genetic and biochemical data	1540:1582	physiological, genetic and biochemical data	1540:1582	On the basis of physiological, genetic and biochemical data, strain YT-3T represented a novel species of the genus Bacillus, for which the name Bacillus ferrooxidans sp.
29948824	8	58	theme	meso-diaminopimelic	993:1011	arg1	acid					1013:1016	meso-diaminopimelic acid	993:1016	meso-diaminopimelic acid	993:1016	The predominant respiratory quinone was Menaquinone-7, and meso-diaminopimelic acid was present in the peptidoglycan layer of cell wall.
29948824	14	59	theme	KCTC	1740:1743	arg1	2017049T					1763:1770	= KCTC 33875T = CCTCC AB 2017049T	1738:1770	= KCTC 33875T = CCTCC AB 2017049T	1738:1770	The type strain is YT-3T (= KCTC 33875T = CCTCC AB 2017049T).
29948824	14	59	theme	KCTC	1740:1743	arg1	YT-3T					1731:1735	YT-3T	1731:1735	YT-3T (= KCTC 33875T = CCTCC AB 2017049T)	1731:1771	The type strain is YT-3T (= KCTC 33875T = CCTCC AB 2017049T).
29948824	5	60	theme	Bacillus	579:586	arg1	21707T					603:608	Bacillus drentensis JCM 21707T	579:608	Bacillus drentensis JCM 21707T	579:608	Analysis of the 16S rRNA gene sequence showed that strain YT-3T was affiliated to the genus Bacillus and displayed the highest similarity to that of Bacillus drentensis JCM 21707T (98.3%), followed by B. ginsengisoli JCM 17335T (97.8%) and B. fumarioli JCM 21708T (97.0%).
29948824	5	61	theme	B.	670:671	arg1	21708T					687:692	B. fumarioli JCM 21708T	670:692	B. fumarioli JCM 21708T (97.0%)	670:700	Analysis of the 16S rRNA gene sequence showed that strain YT-3T was affiliated to the genus Bacillus and displayed the highest similarity to that of Bacillus drentensis JCM 21707T (98.3%), followed by B. ginsengisoli JCM 17335T (97.8%) and B. fumarioli JCM 21708T (97.0%).
29948824	5	61	theme	B.	670:671	arg1	%					699:699	97.0%	695:699	97.0%	695:699	Analysis of the 16S rRNA gene sequence showed that strain YT-3T was affiliated to the genus Bacillus and displayed the highest similarity to that of Bacillus drentensis JCM 21707T (98.3%), followed by B. ginsengisoli JCM 17335T (97.8%) and B. fumarioli JCM 21708T (97.0%).
29948824	6	62	theme	JCM	779:781	arg1	21707T					783:788	B. drentensis JCM 21707T	765:788	B. drentensis JCM 21707T	765:788	The similarity of rpoB gene sequence between strain YT-3T and B. drentensis JCM 21707T, B. ginsengisoli JCM 17335T and B. fumarioli JCM 21708T was 80.4%, 81.5%, and 82.1%, respectively.
29948824	3	63	theme	Gram-positive	226:238	arg1	anaerobic					265:273	anaerobic	265:273	anaerobic	265:273	Cells of strain YT-3T were Gram-positive, rod-shaped, facultative anaerobic, catalase, and oxidase positive.
29948824	10	64	theme	ammoniac	1309:1316	arg1	phospholipids					1318:1330	ammoniac phospholipids	1309:1330	ammoniac phospholipids	1309:1330	The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phospholipids, and ammoniac phospholipids.
29948824	11	65	theme	B.	1454:1455	arg1	fumarioli					1457:1465	B. drentensis (JCM 21707T), B. ginsengisoli (JCM 17335T), and B. fumarioli	1392:1465	B. drentensis (JCM 21707T), B. ginsengisoli (JCM 17335T), and B. fumarioli (JCM 21708T)	1392:1478	The DNA-DNA hybridization values between isolate YT-3T and B. drentensis (JCM 21707T), B. ginsengisoli (JCM 17335T), and B. fumarioli (JCM 21708T) were 36.3%, 30.3%, and 25.3%, respectively.
29948824	11	65	theme	B.	1454:1455	arg1	21708T					1472:1477	JCM 21708T	1468:1477	JCM 21708T	1468:1477	The DNA-DNA hybridization values between isolate YT-3T and B. drentensis (JCM 21707T), B. ginsengisoli (JCM 17335T), and B. fumarioli (JCM 21708T) were 36.3%, 30.3%, and 25.3%, respectively.
29948824	5	66	theme	JCM	599:601	arg1	21707T					603:608	Bacillus drentensis JCM 21707T	579:608	Bacillus drentensis JCM 21707T	579:608	Analysis of the 16S rRNA gene sequence showed that strain YT-3T was affiliated to the genus Bacillus and displayed the highest similarity to that of Bacillus drentensis JCM 21707T (98.3%), followed by B. ginsengisoli JCM 17335T (97.8%) and B. fumarioli JCM 21708T (97.0%).
29948824	5	67	theme	JCM	683:685	arg1	21708T					687:692	B. fumarioli JCM 21708T	670:692	B. fumarioli JCM 21708T (97.0%)	670:700	Analysis of the 16S rRNA gene sequence showed that strain YT-3T was affiliated to the genus Bacillus and displayed the highest similarity to that of Bacillus drentensis JCM 21707T (98.3%), followed by B. ginsengisoli JCM 17335T (97.8%) and B. fumarioli JCM 21708T (97.0%).
29948824	5	67	theme	JCM	683:685	arg1	%					699:699	97.0%	695:699	97.0%	695:699	Analysis of the 16S rRNA gene sequence showed that strain YT-3T was affiliated to the genus Bacillus and displayed the highest similarity to that of Bacillus drentensis JCM 21707T (98.3%), followed by B. ginsengisoli JCM 17335T (97.8%) and B. fumarioli JCM 21708T (97.0%).
29948824	12	68	theme	physiological	1540:1552	arg1	data					1579:1582	physiological, genetic and biochemical data	1540:1582	physiological, genetic and biochemical data	1540:1582	On the basis of physiological, genetic and biochemical data, strain YT-3T represented a novel species of the genus Bacillus, for which the name Bacillus ferrooxidans sp.
29948824	6	69	theme	B.	765:766	arg1	21707T					783:788	B. drentensis JCM 21707T	765:788	B. drentensis JCM 21707T	765:788	The similarity of rpoB gene sequence between strain YT-3T and B. drentensis JCM 21707T, B. ginsengisoli JCM 17335T and B. fumarioli JCM 21708T was 80.4%, 81.5%, and 82.1%, respectively.
29948824	3	70	theme	YT-3T	215:219	arg1	Cells					199:203	Cells	199:203	Cells of strain YT-3T	199:219	Cells of strain YT-3T were Gram-positive, rod-shaped, facultative anaerobic, catalase, and oxidase positive.
29948824	6	71	dep	B.	765:766	arg1	drentensis					768:777	drentensis	768:777	drentensis	768:777	The similarity of rpoB gene sequence between strain YT-3T and B. drentensis JCM 21707T, B. ginsengisoli JCM 17335T and B. fumarioli JCM 21708T was 80.4%, 81.5%, and 82.1%, respectively.
29948824	8	72	theme	predominant	938:948	arg1	quinone					962:968	The predominant respiratory quinone	934:968	The predominant respiratory quinone	934:968	The predominant respiratory quinone was Menaquinone-7, and meso-diaminopimelic acid was present in the peptidoglycan layer of cell wall.
29948824	8	72	theme	predominant	938:948	arg1	Menaquinone-7					974:986	Menaquinone-7	974:986	Menaquinone-7	974:986	The predominant respiratory quinone was Menaquinone-7, and meso-diaminopimelic acid was present in the peptidoglycan layer of cell wall.
29948824	12	73	theme	genetic	1555:1561	arg1	data					1579:1582	physiological, genetic and biochemical data	1540:1582	physiological, genetic and biochemical data	1540:1582	On the basis of physiological, genetic and biochemical data, strain YT-3T represented a novel species of the genus Bacillus, for which the name Bacillus ferrooxidans sp.
29948824	8	74	theme	peptidoglycan	1037:1049	arg1	layer					1051:1055	the peptidoglycan layer	1033:1055	the peptidoglycan layer of cell wall	1033:1068	The predominant respiratory quinone was Menaquinone-7, and meso-diaminopimelic acid was present in the peptidoglycan layer of cell wall.
29948824	5	75	theme	rRNA	450:453	arg1	sequence					460:467	the 16S rRNA gene sequence	442:467	the 16S rRNA gene sequence	442:467	Analysis of the 16S rRNA gene sequence showed that strain YT-3T was affiliated to the genus Bacillus and displayed the highest similarity to that of Bacillus drentensis JCM 21707T (98.3%), followed by B. ginsengisoli JCM 17335T (97.8%) and B. fumarioli JCM 21708T (97.0%).
29948824	6	76	theme	JCM	835:837	arg1	21708T					839:844	B. fumarioli JCM 21708T	822:844	B. fumarioli JCM 21708T	822:844	The similarity of rpoB gene sequence between strain YT-3T and B. drentensis JCM 21707T, B. ginsengisoli JCM 17335T and B. fumarioli JCM 21708T was 80.4%, 81.5%, and 82.1%, respectively.
29948824	12	77	theme	Bacillus	1668:1675	arg1	sp					1690:1691	Bacillus ferrooxidans sp	1668:1691	the name Bacillus ferrooxidans sp	1659:1691	On the basis of physiological, genetic and biochemical data, strain YT-3T represented a novel species of the genus Bacillus, for which the name Bacillus ferrooxidans sp.
29948824	6	78	theme	B.	822:823	arg1	21708T					839:844	B. fumarioli JCM 21708T	822:844	B. fumarioli JCM 21708T	822:844	The similarity of rpoB gene sequence between strain YT-3T and B. drentensis JCM 21707T, B. ginsengisoli JCM 17335T and B. fumarioli JCM 21708T was 80.4%, 81.5%, and 82.1%, respectively.
29948824	12	79	theme	Bacillus	1639:1646	arg1	species					1618:1624	a novel species	1610:1624	a novel species	1610:1624	On the basis of physiological, genetic and biochemical data, strain YT-3T represented a novel species of the genus Bacillus, for which the name Bacillus ferrooxidans sp.
29948824	6	80	theme	ginsengisoli	794:805	arg1	17335T					811:816	B. ginsengisoli JCM 17335T	791:816	B. ginsengisoli JCM 17335T	791:816	The similarity of rpoB gene sequence between strain YT-3T and B. drentensis JCM 21707T, B. ginsengisoli JCM 17335T and B. fumarioli JCM 21708T was 80.4%, 81.5%, and 82.1%, respectively.
29948824	0	81	theme	ferrooxidans	9:20	arg1	sp					22:23	Bacillus ferrooxidans sp	0:23	Bacillus ferrooxidans sp.	0:24	Bacillus ferrooxidans sp.
29948824	9	82	theme	major	1075:1079	arg1	iso					1127:1129	C15:0 anteiso (36.2%), C14:0 iso	1098:1129	C15:0 anteiso (36.2%), C14:0 iso (19.6%)	1098:1137	The major fatty acids were C15:0 anteiso (36.2%), C14:0 iso (19.6%), C15:0 iso (17.4%), and C16:0 iso (9.8%).
29948824	9	82	theme	major	1075:1079	arg1	acids					1087:1091	The major fatty acids	1071:1091	The major fatty acids	1071:1091	The major fatty acids were C15:0 anteiso (36.2%), C14:0 iso (19.6%), C15:0 iso (17.4%), and C16:0 iso (9.8%).
29948824	5	83	theme	sequence	460:467	arg1	Analysis					430:437	Analysis	430:437	Analysis of the 16S rRNA gene sequence	430:467	Analysis of the 16S rRNA gene sequence showed that strain YT-3T was affiliated to the genus Bacillus and displayed the highest similarity to that of Bacillus drentensis JCM 21707T (98.3%), followed by B. ginsengisoli JCM 17335T (97.8%) and B. fumarioli JCM 21708T (97.0%).
29948824	12	84	dep	name	1663:1666	arg1	sp					1690:1691	Bacillus ferrooxidans sp	1668:1691	the name Bacillus ferrooxidans sp	1659:1691	On the basis of physiological, genetic and biochemical data, strain YT-3T represented a novel species of the genus Bacillus, for which the name Bacillus ferrooxidans sp.
29948824	2	85	attach	isolated	144:151	arg1	soil					166:169	a paddy soil	158:169	a paddy soil in Yingtan, Jiangxi, China	158:196	An endospore-forming bacterium, designated YT-3T, was isolated from a paddy soil in Yingtan, Jiangxi, China.
29948824	2	85	attach	isolated	144:151	arg2	bacterium					111:119	An endospore-forming bacterium	90:119	An endospore-forming bacterium	90:119	An endospore-forming bacterium, designated YT-3T, was isolated from a paddy soil in Yingtan, Jiangxi, China.
29948824	5	86	dep	displayed	535:543	arg1	%					615:615	98.3%	611:615	98.3%	611:615	Analysis of the 16S rRNA gene sequence showed that strain YT-3T was affiliated to the genus Bacillus and displayed the highest similarity to that of Bacillus drentensis JCM 21707T (98.3%), followed by B. ginsengisoli JCM 17335T (97.8%) and B. fumarioli JCM 21708T (97.0%).
29948824	11	87	theme	B.	1392:1393	arg1	fumarioli					1457:1465	B. drentensis (JCM 21707T), B. ginsengisoli (JCM 17335T), and B. fumarioli	1392:1465	B. drentensis (JCM 21707T), B. ginsengisoli (JCM 17335T), and B. fumarioli (JCM 21708T)	1392:1478	The DNA-DNA hybridization values between isolate YT-3T and B. drentensis (JCM 21707T), B. ginsengisoli (JCM 17335T), and B. fumarioli (JCM 21708T) were 36.3%, 30.3%, and 25.3%, respectively.
29948824	11	87	theme	B.	1392:1393	arg1	21708T					1472:1477	JCM 21708T	1468:1477	JCM 21708T	1468:1477	The DNA-DNA hybridization values between isolate YT-3T and B. drentensis (JCM 21707T), B. ginsengisoli (JCM 17335T), and B. fumarioli (JCM 21708T) were 36.3%, 30.3%, and 25.3%, respectively.
29948824	5	88	theme	ginsengisoli	634:645	arg1	17335T					651:656	B. ginsengisoli JCM 17335T	631:656	B. ginsengisoli JCM 17335T (97.8%)	631:664	Analysis of the 16S rRNA gene sequence showed that strain YT-3T was affiliated to the genus Bacillus and displayed the highest similarity to that of Bacillus drentensis JCM 21707T (98.3%), followed by B. ginsengisoli JCM 17335T (97.8%) and B. fumarioli JCM 21708T (97.0%).
29948824	5	88	theme	ginsengisoli	634:645	arg1	%					663:663	97.8%	659:663	97.8%	659:663	Analysis of the 16S rRNA gene sequence showed that strain YT-3T was affiliated to the genus Bacillus and displayed the highest similarity to that of Bacillus drentensis JCM 21707T (98.3%), followed by B. ginsengisoli JCM 17335T (97.8%) and B. fumarioli JCM 21708T (97.0%).
29948824	8	89	theme	respiratory	950:960	arg1	quinone					962:968	The predominant respiratory quinone	934:968	The predominant respiratory quinone	934:968	The predominant respiratory quinone was Menaquinone-7, and meso-diaminopimelic acid was present in the peptidoglycan layer of cell wall.
29948824	8	89	theme	respiratory	950:960	arg1	Menaquinone-7					974:986	Menaquinone-7	974:986	Menaquinone-7	974:986	The predominant respiratory quinone was Menaquinone-7, and meso-diaminopimelic acid was present in the peptidoglycan layer of cell wall.
29948824	7	90	theme	DNA	901:903	arg1	%					931:931	44.9 mol%	923:931	44.9 mol%	923:931	The genomic DNA G + C content was 44.9 mol%.
29948824	7	90	theme	DNA	901:903	arg1	G					905:905	The genomic DNA G + C content	889:917	G	905:905	The genomic DNA G + C content was 44.9 mol%.
29948824	4	91	dep	11	411:412	arg1	to					408:409	to	408:409	to	408:409	The optimum growth temperature and pH were 30°C (ranged from 15 to 50°C) and 6.5-7.0 (ranged from 3 to 11), respectively.
29948824	2	92	theme	paddy	160:164	arg1	soil					166:169	a paddy soil	158:169	a paddy soil in Yingtan, Jiangxi, China	158:196	An endospore-forming bacterium, designated YT-3T, was isolated from a paddy soil in Yingtan, Jiangxi, China.
29948824	10	93	theme	lipid	1191:1195	arg1	profile					1197:1203	The polar lipid profile	1181:1203	The polar lipid profile	1181:1203	The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phospholipids, and ammoniac phospholipids.
29948824	2	94	theme	endospore-forming	93:109	arg1	bacterium					111:119	An endospore-forming bacterium	90:119	An endospore-forming bacterium	90:119	An endospore-forming bacterium, designated YT-3T, was isolated from a paddy soil in Yingtan, Jiangxi, China.
31800596	0	0	theme	sinensis	78:85	arg1	composition					25:35	the composition	21:35	the composition of cultivated, naturally grown Cordyceps sinensis, and stiff worms across different sampling years	21:134	Comparative study of the composition of cultivated, naturally grown Cordyceps sinensis, and stiff worms across different sampling years.
31800596	5	1	from	difference	805:814	arg1	content					847:853	the average soluble protein content	819:853	the average soluble protein content	819:853	No distinct difference in the average soluble protein content was observed.
31800596	1	2	theme	Cordyceps	145:153	arg1	agent					229:233	a valuable anti-tumor, immunomodulatory, and antiviral agent	174:233	a valuable anti-tumor, immunomodulatory, and antiviral agent in Asia	174:241	Natural Cordyceps sinensis, which is a valuable anti-tumor, immunomodulatory, and antiviral agent in Asia, has been overexploited in recent years.
31800596	1	2	theme	Cordyceps	145:153	arg1	sinensis					155:162	Natural Cordyceps sinensis	137:162	Natural Cordyceps sinensis	137:162	Natural Cordyceps sinensis, which is a valuable anti-tumor, immunomodulatory, and antiviral agent in Asia, has been overexploited in recent years.
31800596	6	3	theme	natural	931:937	arg1	herb					939:942	the natural herb	927:942	the natural herb	927:942	The composition of the stiff worms was similar to that of the natural herb, except that the total soluble protein content was higher, and that of mannitol was lower.
31800596	0	4	theme	Cordyceps	68:76	arg1	sinensis					78:85	naturally grown Cordyceps sinensis	52:85	naturally grown Cordyceps sinensis	52:85	Comparative study of the composition of cultivated, naturally grown Cordyceps sinensis, and stiff worms across different sampling years.
31800596	5	5	theme	protein	839:845	arg1	content					847:853	the average soluble protein content	819:853	the average soluble protein content	819:853	No distinct difference in the average soluble protein content was observed.
31800596	2	6	theme	cultivated	315:324	arg1	sinensis					329:336	cultivated C. sinensis	315:336	cultivated C. sinensis	315:336	Therefore, it is important for cultivated C. sinensis to be recognized in the market.
31800596	3	7	theme	different	484:492	arg1	years					503:507	different sampling years	484:507	different sampling years	484:507	In this research, the main components of entirely cultivated, naturally grown C. sinensis, and stiff worms across different sampling years were detected and compared by HPLC-MS and UV spectrometry.
31800596	7	8	theme	types	1101:1105	arg1	spectroscopy					1075:1086	the ultraviolet absorption spectroscopy	1048:1086	the ultraviolet absorption spectroscopy of the three types	1048:1105	In addition, the ultraviolet absorption spectroscopy of the three types showed high similarity at 260 nm.
31800596	8	9	theme	cultivated	1206:1215	arg1	sinensis					1232:1239	cultivated and natural C. sinensis	1206:1239	cultivated and natural C. sinensis	1206:1239	This research indicates that the main nutritional composition of cultivated and natural C. sinensis is identical and that cultivated type can be used as an effective substitute.
31800596	3	10	theme	sampling	494:501	arg1	years					503:507	different sampling years	484:507	different sampling years	484:507	In this research, the main components of entirely cultivated, naturally grown C. sinensis, and stiff worms across different sampling years were detected and compared by HPLC-MS and UV spectrometry.
31800596	2	11	theme	C.	326:327	arg1	sinensis					329:336	cultivated C. sinensis	315:336	cultivated C. sinensis	315:336	Therefore, it is important for cultivated C. sinensis to be recognized in the market.
31800596	8	12	used	used	1286:1289	arg2	type					1274:1277	cultivated type	1263:1277	cultivated type	1263:1277	This research indicates that the main nutritional composition of cultivated and natural C. sinensis is identical and that cultivated type can be used as an effective substitute.
31800596	8	12	used	used	1286:1289	arg2	effective					1297:1305	effective	1297:1305	effective	1297:1305	This research indicates that the main nutritional composition of cultivated and natural C. sinensis is identical and that cultivated type can be used as an effective substitute.
31800596	0	13	theme	worms	98:102	arg1	composition					25:35	the composition	21:35	the composition of cultivated, naturally grown Cordyceps sinensis, and stiff worms across different sampling years	21:134	Comparative study of the composition of cultivated, naturally grown Cordyceps sinensis, and stiff worms across different sampling years.
31800596	3	14	theme	cultivated	420:429	arg1	components					397:406	the main components	388:406	the main components of entirely cultivated, naturally grown C. sinensis, and stiff worms across different sampling years	388:507	In this research, the main components of entirely cultivated, naturally grown C. sinensis, and stiff worms across different sampling years were detected and compared by HPLC-MS and UV spectrometry.
31800596	3	15	theme	worms	471:475	arg1	components					397:406	the main components	388:406	the main components of entirely cultivated, naturally grown C. sinensis, and stiff worms across different sampling years	388:507	In this research, the main components of entirely cultivated, naturally grown C. sinensis, and stiff worms across different sampling years were detected and compared by HPLC-MS and UV spectrometry.
31800596	3	16	located	detected	514:521	arg1	research					378:385	this research	373:385	this research	373:385	In this research, the main components of entirely cultivated, naturally grown C. sinensis, and stiff worms across different sampling years were detected and compared by HPLC-MS and UV spectrometry.
31800596	3	16	located	detected	514:521	arg2	components					397:406	the main components	388:406	the main components of entirely cultivated, naturally grown C. sinensis, and stiff worms across different sampling years	388:507	In this research, the main components of entirely cultivated, naturally grown C. sinensis, and stiff worms across different sampling years were detected and compared by HPLC-MS and UV spectrometry.
31800596	5	17	theme	distinct	796:803	arg1	difference					805:814	No distinct difference	793:814	No distinct difference in the average soluble protein content	793:853	No distinct difference in the average soluble protein content was observed.
31800596	0	18	theme	stiff	92:96	arg1	worms					98:102	stiff worms	92:102	stiff worms	92:102	Comparative study of the composition of cultivated, naturally grown Cordyceps sinensis, and stiff worms across different sampling years.
31800596	4	19	theme	natural	779:785	arg1	type					787:790	the natural type	775:790	the natural type	775:790	The results indicated that the mean levels of adenosine and cordycepin were significantly higher, whereas the mean levels of mannitol and polysaccharides were remarkably lower in the cultivated type than in the natural type.
31800596	3	20	theme	main	392:395	arg1	components					397:406	the main components	388:406	the main components of entirely cultivated, naturally grown C. sinensis, and stiff worms across different sampling years	388:507	In this research, the main components of entirely cultivated, naturally grown C. sinensis, and stiff worms across different sampling years were detected and compared by HPLC-MS and UV spectrometry.
31800596	6	21	theme	worms	898:902	arg1	composition					873:883	The composition	869:883	The composition of the stiff worms	869:902	The composition of the stiff worms was similar to that of the natural herb, except that the total soluble protein content was higher, and that of mannitol was lower.
31800596	6	21	theme	worms	898:902	arg1	similar					908:914	similar	908:914	similar	908:914	The composition of the stiff worms was similar to that of the natural herb, except that the total soluble protein content was higher, and that of mannitol was lower.
31800596	4	22	theme	adenosine	614:622	arg1	levels					604:609	the mean levels	595:609	the mean levels of adenosine and cordycepin	595:637	The results indicated that the mean levels of adenosine and cordycepin were significantly higher, whereas the mean levels of mannitol and polysaccharides were remarkably lower in the cultivated type than in the natural type.
31800596	4	22	theme	adenosine	614:622	arg1	higher					658:663	higher	658:663	higher	658:663	The results indicated that the mean levels of adenosine and cordycepin were significantly higher, whereas the mean levels of mannitol and polysaccharides were remarkably lower in the cultivated type than in the natural type.
31800596	0	23	theme	Comparative	0:10	arg1	study					12:16	Comparative study	0:16	Comparative study of the composition of cultivated, naturally grown Cordyceps sinensis, and stiff worms across different sampling years.	0:135	Comparative study of the composition of cultivated, naturally grown Cordyceps sinensis, and stiff worms across different sampling years.
31800596	7	24	theme	ultraviolet	1052:1062	arg1	spectroscopy					1075:1086	the ultraviolet absorption spectroscopy	1048:1086	the ultraviolet absorption spectroscopy of the three types	1048:1105	In addition, the ultraviolet absorption spectroscopy of the three types showed high similarity at 260 nm.
31800596	4	25	theme	mean	678:681	arg1	lower					738:742	lower	738:742	lower	738:742	The results indicated that the mean levels of adenosine and cordycepin were significantly higher, whereas the mean levels of mannitol and polysaccharides were remarkably lower in the cultivated type than in the natural type.
31800596	4	25	theme	mean	678:681	arg1	levels					683:688	the mean levels	674:688	the mean levels of mannitol	674:700	The results indicated that the mean levels of adenosine and cordycepin were significantly higher, whereas the mean levels of mannitol and polysaccharides were remarkably lower in the cultivated type than in the natural type.
31800596	7	26	theme	absorption	1064:1073	arg1	spectroscopy					1075:1086	the ultraviolet absorption spectroscopy	1048:1086	the ultraviolet absorption spectroscopy of the three types	1048:1105	In addition, the ultraviolet absorption spectroscopy of the three types showed high similarity at 260 nm.
31800596	4	27	theme	cultivated	751:760	arg1	type					762:765	the cultivated type	747:765	the cultivated type	747:765	The results indicated that the mean levels of adenosine and cordycepin were significantly higher, whereas the mean levels of mannitol and polysaccharides were remarkably lower in the cultivated type than in the natural type.
31800596	0	28	theme	different	111:119	arg1	years					130:134	different sampling years	111:134	different sampling years	111:134	Comparative study of the composition of cultivated, naturally grown Cordyceps sinensis, and stiff worms across different sampling years.
31800596	4	29	theme	mannitol	693:700	arg1	polysaccharides					706:720	polysaccharides	706:720	polysaccharides	706:720	The results indicated that the mean levels of adenosine and cordycepin were significantly higher, whereas the mean levels of mannitol and polysaccharides were remarkably lower in the cultivated type than in the natural type.
31800596	4	29	theme	mannitol	693:700	arg1	lower					738:742	lower	738:742	lower	738:742	The results indicated that the mean levels of adenosine and cordycepin were significantly higher, whereas the mean levels of mannitol and polysaccharides were remarkably lower in the cultivated type than in the natural type.
31800596	4	29	theme	mannitol	693:700	arg1	levels					683:688	the mean levels	674:688	the mean levels of mannitol	674:700	The results indicated that the mean levels of adenosine and cordycepin were significantly higher, whereas the mean levels of mannitol and polysaccharides were remarkably lower in the cultivated type than in the natural type.
31800596	1	30	theme	valuable	176:183	arg1	agent					229:233	a valuable anti-tumor, immunomodulatory, and antiviral agent	174:233	a valuable anti-tumor, immunomodulatory, and antiviral agent in Asia	174:241	Natural Cordyceps sinensis, which is a valuable anti-tumor, immunomodulatory, and antiviral agent in Asia, has been overexploited in recent years.
31800596	1	30	theme	valuable	176:183	arg1	sinensis					155:162	Natural Cordyceps sinensis	137:162	Natural Cordyceps sinensis	137:162	Natural Cordyceps sinensis, which is a valuable anti-tumor, immunomodulatory, and antiviral agent in Asia, has been overexploited in recent years.
31800596	8	31	theme	cultivated	1263:1272	arg1	effective					1297:1305	effective	1297:1305	effective	1297:1305	This research indicates that the main nutritional composition of cultivated and natural C. sinensis is identical and that cultivated type can be used as an effective substitute.
31800596	8	31	theme	cultivated	1263:1272	arg1	type					1274:1277	cultivated type	1263:1277	cultivated type	1263:1277	This research indicates that the main nutritional composition of cultivated and natural C. sinensis is identical and that cultivated type can be used as an effective substitute.
31800596	8	32	theme	sinensis	1232:1239	arg1	composition					1191:1201	the main nutritional composition	1170:1201	the main nutritional composition of cultivated and natural C. sinensis	1170:1239	This research indicates that the main nutritional composition of cultivated and natural C. sinensis is identical and that cultivated type can be used as an effective substitute.
31800596	8	32	theme	sinensis	1232:1239	arg1	identical					1244:1252	identical	1244:1252	identical	1244:1252	This research indicates that the main nutritional composition of cultivated and natural C. sinensis is identical and that cultivated type can be used as an effective substitute.
31800596	1	33	theme	anti-tumor	185:194	arg1	agent					229:233	a valuable anti-tumor, immunomodulatory, and antiviral agent	174:233	a valuable anti-tumor, immunomodulatory, and antiviral agent in Asia	174:241	Natural Cordyceps sinensis, which is a valuable anti-tumor, immunomodulatory, and antiviral agent in Asia, has been overexploited in recent years.
31800596	1	33	theme	anti-tumor	185:194	arg1	sinensis					155:162	Natural Cordyceps sinensis	137:162	Natural Cordyceps sinensis	137:162	Natural Cordyceps sinensis, which is a valuable anti-tumor, immunomodulatory, and antiviral agent in Asia, has been overexploited in recent years.
31800596	3	34	theme	sinensis	451:458	arg1	components					397:406	the main components	388:406	the main components of entirely cultivated, naturally grown C. sinensis, and stiff worms across different sampling years	388:507	In this research, the main components of entirely cultivated, naturally grown C. sinensis, and stiff worms across different sampling years were detected and compared by HPLC-MS and UV spectrometry.
31800596	1	35	theme	recent	270:275	arg1	years					277:281	recent years	270:281	recent years	270:281	Natural Cordyceps sinensis, which is a valuable anti-tumor, immunomodulatory, and antiviral agent in Asia, has been overexploited in recent years.
31800596	8	36	theme	nutritional	1179:1189	arg1	composition					1191:1201	the main nutritional composition	1170:1201	the main nutritional composition of cultivated and natural C. sinensis	1170:1239	This research indicates that the main nutritional composition of cultivated and natural C. sinensis is identical and that cultivated type can be used as an effective substitute.
31800596	8	36	theme	nutritional	1179:1189	arg1	identical					1244:1252	identical	1244:1252	identical	1244:1252	This research indicates that the main nutritional composition of cultivated and natural C. sinensis is identical and that cultivated type can be used as an effective substitute.
31800596	6	37	theme	total	961:965	arg1	higher					995:1000	higher	995:1000	higher	995:1000	The composition of the stiff worms was similar to that of the natural herb, except that the total soluble protein content was higher, and that of mannitol was lower.
31800596	6	37	theme	total	961:965	arg1	content					983:989	the total soluble protein content	957:989	the total soluble protein content	957:989	The composition of the stiff worms was similar to that of the natural herb, except that the total soluble protein content was higher, and that of mannitol was lower.
31800596	0	38	theme	composition	25:35	arg1	study					12:16	Comparative study	0:16	Comparative study of the composition of cultivated, naturally grown Cordyceps sinensis, and stiff worms across different sampling years.	0:135	Comparative study of the composition of cultivated, naturally grown Cordyceps sinensis, and stiff worms across different sampling years.
31800596	6	39	theme	stiff	892:896	arg1	worms					898:902	the stiff worms	888:902	the stiff worms	888:902	The composition of the stiff worms was similar to that of the natural herb, except that the total soluble protein content was higher, and that of mannitol was lower.
31800596	0	40	theme	sampling	121:128	arg1	years					130:134	different sampling years	111:134	different sampling years	111:134	Comparative study of the composition of cultivated, naturally grown Cordyceps sinensis, and stiff worms across different sampling years.
31800596	6	41	theme	soluble	967:973	arg1	higher					995:1000	higher	995:1000	higher	995:1000	The composition of the stiff worms was similar to that of the natural herb, except that the total soluble protein content was higher, and that of mannitol was lower.
31800596	6	41	theme	soluble	967:973	arg1	content					983:989	the total soluble protein content	957:989	the total soluble protein content	957:989	The composition of the stiff worms was similar to that of the natural herb, except that the total soluble protein content was higher, and that of mannitol was lower.
31800596	1	42	theme	immunomodulatory	197:212	arg1	agent					229:233	a valuable anti-tumor, immunomodulatory, and antiviral agent	174:233	a valuable anti-tumor, immunomodulatory, and antiviral agent in Asia	174:241	Natural Cordyceps sinensis, which is a valuable anti-tumor, immunomodulatory, and antiviral agent in Asia, has been overexploited in recent years.
31800596	1	42	theme	immunomodulatory	197:212	arg1	sinensis					155:162	Natural Cordyceps sinensis	137:162	Natural Cordyceps sinensis	137:162	Natural Cordyceps sinensis, which is a valuable anti-tumor, immunomodulatory, and antiviral agent in Asia, has been overexploited in recent years.
31800596	5	43	theme	average	823:829	arg1	content					847:853	the average soluble protein content	819:853	the average soluble protein content	819:853	No distinct difference in the average soluble protein content was observed.
31800596	8	44	dep	effective	1297:1305	arg1	substitute					1307:1316	substitute	1307:1316	substitute	1307:1316	This research indicates that the main nutritional composition of cultivated and natural C. sinensis is identical and that cultivated type can be used as an effective substitute.
31800596	8	45	theme	natural	1221:1227	arg1	sinensis					1232:1239	cultivated and natural C. sinensis	1206:1239	cultivated and natural C. sinensis	1206:1239	This research indicates that the main nutritional composition of cultivated and natural C. sinensis is identical and that cultivated type can be used as an effective substitute.
31800596	0	46	theme	cultivated	40:49	arg1	composition					25:35	the composition	21:35	the composition of cultivated, naturally grown Cordyceps sinensis, and stiff worms across different sampling years	21:134	Comparative study of the composition of cultivated, naturally grown Cordyceps sinensis, and stiff worms across different sampling years.
31800596	1	47	from	agent	229:233	arg1	Asia					238:241	Asia	238:241	Asia	238:241	Natural Cordyceps sinensis, which is a valuable anti-tumor, immunomodulatory, and antiviral agent in Asia, has been overexploited in recent years.
31800596	8	48	theme	main	1174:1177	arg1	composition					1191:1201	the main nutritional composition	1170:1201	the main nutritional composition of cultivated and natural C. sinensis	1170:1239	This research indicates that the main nutritional composition of cultivated and natural C. sinensis is identical and that cultivated type can be used as an effective substitute.
31800596	8	48	theme	main	1174:1177	arg1	identical					1244:1252	identical	1244:1252	identical	1244:1252	This research indicates that the main nutritional composition of cultivated and natural C. sinensis is identical and that cultivated type can be used as an effective substitute.
31800596	8	49	theme	C.	1229:1230	arg1	sinensis					1232:1239	cultivated and natural C. sinensis	1206:1239	cultivated and natural C. sinensis	1206:1239	This research indicates that the main nutritional composition of cultivated and natural C. sinensis is identical and that cultivated type can be used as an effective substitute.
31800596	3	50	theme	stiff	465:469	arg1	worms					471:475	stiff worms	465:475	stiff worms	465:475	In this research, the main components of entirely cultivated, naturally grown C. sinensis, and stiff worms across different sampling years were detected and compared by HPLC-MS and UV spectrometry.
31800596	0	51	theme	grown	62:66	arg1	sinensis					78:85	naturally grown Cordyceps sinensis	52:85	naturally grown Cordyceps sinensis	52:85	Comparative study of the composition of cultivated, naturally grown Cordyceps sinensis, and stiff worms across different sampling years.
31800596	5	52	theme	soluble	831:837	arg1	content					847:853	the average soluble protein content	819:853	the average soluble protein content	819:853	No distinct difference in the average soluble protein content was observed.
31800596	3	53	theme	UV	551:552	arg1	spectrometry					554:565	UV spectrometry	551:565	UV spectrometry	551:565	In this research, the main components of entirely cultivated, naturally grown C. sinensis, and stiff worms across different sampling years were detected and compared by HPLC-MS and UV spectrometry.
31800596	6	54	theme	protein	975:981	arg1	higher					995:1000	higher	995:1000	higher	995:1000	The composition of the stiff worms was similar to that of the natural herb, except that the total soluble protein content was higher, and that of mannitol was lower.
31800596	6	54	theme	protein	975:981	arg1	content					983:989	the total soluble protein content	957:989	the total soluble protein content	957:989	The composition of the stiff worms was similar to that of the natural herb, except that the total soluble protein content was higher, and that of mannitol was lower.
31800596	4	55	theme	cordycepin	628:637	arg1	levels					604:609	the mean levels	595:609	the mean levels of adenosine and cordycepin	595:637	The results indicated that the mean levels of adenosine and cordycepin were significantly higher, whereas the mean levels of mannitol and polysaccharides were remarkably lower in the cultivated type than in the natural type.
31800596	4	55	theme	cordycepin	628:637	arg1	higher					658:663	higher	658:663	higher	658:663	The results indicated that the mean levels of adenosine and cordycepin were significantly higher, whereas the mean levels of mannitol and polysaccharides were remarkably lower in the cultivated type than in the natural type.
31800596	4	56	theme	mean	599:602	arg1	levels					604:609	the mean levels	595:609	the mean levels of adenosine and cordycepin	595:637	The results indicated that the mean levels of adenosine and cordycepin were significantly higher, whereas the mean levels of mannitol and polysaccharides were remarkably lower in the cultivated type than in the natural type.
31800596	4	56	theme	mean	599:602	arg1	higher					658:663	higher	658:663	higher	658:663	The results indicated that the mean levels of adenosine and cordycepin were significantly higher, whereas the mean levels of mannitol and polysaccharides were remarkably lower in the cultivated type than in the natural type.
31800596	1	57	theme	antiviral	219:227	arg1	agent					229:233	a valuable anti-tumor, immunomodulatory, and antiviral agent	174:233	a valuable anti-tumor, immunomodulatory, and antiviral agent in Asia	174:241	Natural Cordyceps sinensis, which is a valuable anti-tumor, immunomodulatory, and antiviral agent in Asia, has been overexploited in recent years.
31800596	1	57	theme	antiviral	219:227	arg1	sinensis					155:162	Natural Cordyceps sinensis	137:162	Natural Cordyceps sinensis	137:162	Natural Cordyceps sinensis, which is a valuable anti-tumor, immunomodulatory, and antiviral agent in Asia, has been overexploited in recent years.
31800596	1	58	theme	Natural	137:143	arg1	agent					229:233	a valuable anti-tumor, immunomodulatory, and antiviral agent	174:233	a valuable anti-tumor, immunomodulatory, and antiviral agent in Asia	174:241	Natural Cordyceps sinensis, which is a valuable anti-tumor, immunomodulatory, and antiviral agent in Asia, has been overexploited in recent years.
31800596	1	58	theme	Natural	137:143	arg1	sinensis					155:162	Natural Cordyceps sinensis	137:162	Natural Cordyceps sinensis	137:162	Natural Cordyceps sinensis, which is a valuable anti-tumor, immunomodulatory, and antiviral agent in Asia, has been overexploited in recent years.
31800596	7	59	theme	high	1114:1117	arg1	similarity					1119:1128	high similarity	1114:1128	high similarity	1114:1128	In addition, the ultraviolet absorption spectroscopy of the three types showed high similarity at 260 nm.
29867910	8	0	theme	strain	923:928	arg1	NCFM					930:933	strain NCFM	923:933	strain NCFM	923:933	The activities of β-GAL, LDH, hexokinase (HK), 6-phosphofructokinase (PFK), and pyruvate kinase (PK) of strain NCFM significantly increased under those conditions.
29867910	11	1	theme	carbon	1281:1286	arg1	metabolism					1288:1297	carbon metabolism	1281:1297	carbon metabolism	1281:1297	Pyruvate metabolism, carbon metabolism, phosphotransferase system (PTS), and glycolysis/gluconeogenesis genes were overexpressed.
29867910	1	2	theme	goji	153:156	arg1	berries					158:164	the goji berries	149:164	the goji berries	149:164	Lycium barbarum is a boxthorn that produces the goji berries.
29867910	10	3	theme	metabolic	1178:1186	arg1	processes					1188:1196	metabolic processes	1178:1196	metabolic processes	1178:1196	The data revealed that LBP promoted the bacterial biosynthetic and metabolic processes, gene expression, transcription, and transmembrane transport.
29867910	12	4	theme	environment	1489:1499	arg1	freeze-drying					1437:1449	freeze-drying	1437:1449	freeze-drying	1437:1449	Furthermore, LBP improved cell vitality during freeze-drying and tolerance of the gastrointestinal environment.
29867910	12	4	theme	environment	1489:1499	arg1	tolerance					1455:1463	tolerance	1455:1463	tolerance	1455:1463	Furthermore, LBP improved cell vitality during freeze-drying and tolerance of the gastrointestinal environment.
29867910	11	5	theme	glycolysis/gluconeogenesis	1337:1362	arg1	genes					1364:1368	glycolysis/gluconeogenesis genes	1337:1368	glycolysis/gluconeogenesis genes	1337:1368	Pyruvate metabolism, carbon metabolism, phosphotransferase system (PTS), and glycolysis/gluconeogenesis genes were overexpressed.
29867910	8	6	theme	kinase	908:913	arg1	activities					823:832	The activities	819:832	The activities of β-GAL, LDH, hexokinase (HK), 6-phosphofructokinase (PFK), and pyruvate kinase (PK) of strain NCFM	819:933	The activities of β-GAL, LDH, hexokinase (HK), 6-phosphofructokinase (PFK), and pyruvate kinase (PK) of strain NCFM significantly increased under those conditions.
29867910	8	7	theme	pyruvate	899:906	arg1	PK					916:917	PK	916:917	PK	916:917	The activities of β-GAL, LDH, hexokinase (HK), 6-phosphofructokinase (PFK), and pyruvate kinase (PK) of strain NCFM significantly increased under those conditions.
29867910	8	7	theme	pyruvate	899:906	arg1	kinase					908:913	pyruvate kinase	899:913	pyruvate kinase (PK)	899:918	The activities of β-GAL, LDH, hexokinase (HK), 6-phosphofructokinase (PFK), and pyruvate kinase (PK) of strain NCFM significantly increased under those conditions.
29867910	13	8	theme	potential	1535:1543	arg1	LBP					1514:1516	LBP	1514:1516	LBP	1514:1516	In summary, LBP can be used as a potential prebiotic for Bifidobacterium and Lactobacillus.
29867910	13	8	theme	potential	1535:1543	arg1	prebiotic					1545:1553	a potential prebiotic	1533:1553	a potential prebiotic for Bifidobacterium and Lactobacillus	1533:1591	In summary, LBP can be used as a potential prebiotic for Bifidobacterium and Lactobacillus.
29867910	8	9	theme	NCFM	930:933	arg1	LDH					844:846	LDH	844:846	LDH	844:846	The activities of β-GAL, LDH, hexokinase (HK), 6-phosphofructokinase (PFK), and pyruvate kinase (PK) of strain NCFM significantly increased under those conditions.
29867910	8	9	theme	NCFM	930:933	arg1	6-phosphofructokinase					866:886	6-phosphofructokinase	866:886	6-phosphofructokinase (PFK)	866:892	The activities of β-GAL, LDH, hexokinase (HK), 6-phosphofructokinase (PFK), and pyruvate kinase (PK) of strain NCFM significantly increased under those conditions.
29867910	8	9	theme	NCFM	930:933	arg1	kinase					908:913	pyruvate kinase	899:913	pyruvate kinase (PK)	899:918	The activities of β-GAL, LDH, hexokinase (HK), 6-phosphofructokinase (PFK), and pyruvate kinase (PK) of strain NCFM significantly increased under those conditions.
29867910	8	9	theme	NCFM	930:933	arg1	PFK					889:891	PFK	889:891	PFK	889:891	The activities of β-GAL, LDH, hexokinase (HK), 6-phosphofructokinase (PFK), and pyruvate kinase (PK) of strain NCFM significantly increased under those conditions.
29867910	8	9	theme	NCFM	930:933	arg1	hexokinase					849:858	hexokinase	849:858	hexokinase (HK)	849:863	The activities of β-GAL, LDH, hexokinase (HK), 6-phosphofructokinase (PFK), and pyruvate kinase (PK) of strain NCFM significantly increased under those conditions.
29867910	8	9	theme	NCFM	930:933	arg1	PK					916:917	PK	916:917	PK	916:917	The activities of β-GAL, LDH, hexokinase (HK), 6-phosphofructokinase (PFK), and pyruvate kinase (PK) of strain NCFM significantly increased under those conditions.
29867910	8	9	theme	NCFM	930:933	arg1	HK					861:862	HK	861:862	HK	861:862	The activities of β-GAL, LDH, hexokinase (HK), 6-phosphofructokinase (PFK), and pyruvate kinase (PK) of strain NCFM significantly increased under those conditions.
29867910	8	9	theme	NCFM	930:933	arg1	β-GAL					837:841	β-GAL	837:841	β-GAL	837:841	The activities of β-GAL, LDH, hexokinase (HK), 6-phosphofructokinase (PFK), and pyruvate kinase (PK) of strain NCFM significantly increased under those conditions.
29867910	6	10	dep	NCFM	656:659	arg1	acidophilus					644:654	Lactobacillus acidophilus	630:654	Lactobacillus acidophilus	630:654	infantis Bi-26 and Lactobacillus acidophilus NCFM.
29867910	6	10	dep	NCFM	656:659	arg1	infantis					611:618	infantis	611:618	infantis	611:618	infantis Bi-26 and Lactobacillus acidophilus NCFM.
29867910	7	11	theme	LBP	681:683	arg1	presence					669:676	the presence	665:676	the presence of LBP in the growth medium	665:704	In the presence of LBP in the growth medium, the β-galactosidase (β-GAL) and lactate dehydrogenase (LDH) activities of strain Bi-26 significantly increased.
29867910	3	12	theme	goji	312:315	arg1	berries					317:323	goji berries	312:323	goji berries	312:323	LBP was extracted from goji berries and its monosaccharide composition characterized by gas chromatography (GC).
29867910	5	13	theme	strains	561:567	arg1	proliferation					517:529	the proliferation	513:529	the proliferation of lactic acid bacteria (LAB) strains, especially Bifidobacterium longum subsp	513:608	LBP obviously promoted the proliferation of lactic acid bacteria (LAB) strains, especially Bifidobacterium longum subsp.
29867910	2	14	theme	polysaccharides	252:266	arg1	effect					230:235	the proliferative effect	212:235	the proliferative effect of L. barbarum polysaccharides (LBP) on probiotics	212:286	The aim of the current study was to evaluate the proliferative effect of L. barbarum polysaccharides (LBP) on probiotics.
29867910	9	15	theme	LAB	983:985	arg1	analysis					1012:1019	LAB transcriptome sequencing analysis	983:1019	LAB transcriptome sequencing analysis	983:1019	LAB transcriptome sequencing analysis was performed to elucidate the mechanism responsible for the proliferative effect of LBP.
29867910	7	16	from	presence	669:676	arg1	medium					699:704	the growth medium	688:704	the growth medium	688:704	In the presence of LBP in the growth medium, the β-galactosidase (β-GAL) and lactate dehydrogenase (LDH) activities of strain Bi-26 significantly increased.
29867910	2	17	theme	barbarum	243:250	arg1	LBP					269:271	LBP	269:271	LBP	269:271	The aim of the current study was to evaluate the proliferative effect of L. barbarum polysaccharides (LBP) on probiotics.
29867910	2	17	theme	barbarum	243:250	arg1	polysaccharides					252:266	L. barbarum polysaccharides	240:266	L. barbarum polysaccharides (LBP)	240:272	The aim of the current study was to evaluate the proliferative effect of L. barbarum polysaccharides (LBP) on probiotics.
29867910	7	18	theme	strain	781:786	arg1	Bi-26					788:792	strain Bi-26	781:792	strain Bi-26	781:792	In the presence of LBP in the growth medium, the β-galactosidase (β-GAL) and lactate dehydrogenase (LDH) activities of strain Bi-26 significantly increased.
29867910	5	19	theme	lactic	534:539	arg1	LAB					556:558	LAB	556:558	LAB	556:558	LBP obviously promoted the proliferation of lactic acid bacteria (LAB) strains, especially Bifidobacterium longum subsp.
29867910	5	19	theme	lactic	534:539	arg1	bacteria					546:553	lactic acid bacteria	534:553	lactic acid bacteria (LAB) strains	534:567	LBP obviously promoted the proliferation of lactic acid bacteria (LAB) strains, especially Bifidobacterium longum subsp.
29867910	7	20	theme	Bi-26	788:792	arg1	activities					767:776	the β-galactosidase (β-GAL) and lactate dehydrogenase (LDH) activities	707:776	the β-galactosidase (β-GAL) and lactate dehydrogenase (LDH) activities of strain Bi-26	707:792	In the presence of LBP in the growth medium, the β-galactosidase (β-GAL) and lactate dehydrogenase (LDH) activities of strain Bi-26 significantly increased.
29867910	0	21	theme	Lyciumbarbarum	0:13	arg1	LBP					31:33	LBP	31:33	LBP	31:33	Lyciumbarbarum Polysaccharide (LBP): A Novel Prebiotics Candidate for Bifidobacterium and Lactobacillus.
29867910	0	21	theme	Lyciumbarbarum	0:13	arg1	Polysaccharide					15:28	Lyciumbarbarum Polysaccharide	0:28	Lyciumbarbarum Polysaccharide (LBP): A Novel Prebiotics Candidate for Bifidobacterium and Lactobacillus.	0:103	Lyciumbarbarum Polysaccharide (LBP): A Novel Prebiotics Candidate for Bifidobacterium and Lactobacillus.
29867910	7	22	theme	dehydrogenase	747:759	arg1	activities					767:776	the β-galactosidase (β-GAL) and lactate dehydrogenase (LDH) activities	707:776	the β-galactosidase (β-GAL) and lactate dehydrogenase (LDH) activities of strain Bi-26	707:792	In the presence of LBP in the growth medium, the β-galactosidase (β-GAL) and lactate dehydrogenase (LDH) activities of strain Bi-26 significantly increased.
29867910	5	23	theme	acid	541:544	arg1	LAB					556:558	LAB	556:558	LAB	556:558	LBP obviously promoted the proliferation of lactic acid bacteria (LAB) strains, especially Bifidobacterium longum subsp.
29867910	5	23	theme	acid	541:544	arg1	bacteria					546:553	lactic acid bacteria	534:553	lactic acid bacteria (LAB) strains	534:567	LBP obviously promoted the proliferation of lactic acid bacteria (LAB) strains, especially Bifidobacterium longum subsp.
29867910	2	24	from	effect	230:235	arg1	probiotics					277:286	probiotics	277:286	probiotics	277:286	The aim of the current study was to evaluate the proliferative effect of L. barbarum polysaccharides (LBP) on probiotics.
29867910	3	25	theme	monosaccharide	333:346	arg1	composition					348:358	its monosaccharide composition	329:358	its monosaccharide composition	329:358	LBP was extracted from goji berries and its monosaccharide composition characterized by gas chromatography (GC).
29867910	10	26	dep	biosynthetic	1161:1172	arg1	transport					1249:1257	transport	1249:1257	transport	1249:1257	The data revealed that LBP promoted the bacterial biosynthetic and metabolic processes, gene expression, transcription, and transmembrane transport.
29867910	5	27	theme	bacteria	546:553	arg1	longum					597:602	Bifidobacterium longum	581:602	Bifidobacterium longum subsp	581:608	LBP obviously promoted the proliferation of lactic acid bacteria (LAB) strains, especially Bifidobacterium longum subsp.
29867910	5	27	theme	bacteria	546:553	arg1	strains					561:567	lactic acid bacteria (LAB) strains	534:567	lactic acid bacteria (LAB) strains	534:567	LBP obviously promoted the proliferation of lactic acid bacteria (LAB) strains, especially Bifidobacterium longum subsp.
29867910	8	28	theme	β-GAL	837:841	arg1	activities					823:832	The activities	819:832	The activities of β-GAL, LDH, hexokinase (HK), 6-phosphofructokinase (PFK), and pyruvate kinase (PK) of strain NCFM	819:933	The activities of β-GAL, LDH, hexokinase (HK), 6-phosphofructokinase (PFK), and pyruvate kinase (PK) of strain NCFM significantly increased under those conditions.
29867910	7	29	attach	presence	669:676	arg1	medium					699:704	the growth medium	688:704	the growth medium	688:704	In the presence of LBP in the growth medium, the β-galactosidase (β-GAL) and lactate dehydrogenase (LDH) activities of strain Bi-26 significantly increased.
29867910	7	29	attach	presence	669:676	arg2	LBP					681:683	LBP	681:683	LBP	681:683	In the presence of LBP in the growth medium, the β-galactosidase (β-GAL) and lactate dehydrogenase (LDH) activities of strain Bi-26 significantly increased.
29867910	11	30	theme	Pyruvate	1260:1267	arg1	metabolism					1269:1278	Pyruvate metabolism	1260:1278	Pyruvate metabolism	1260:1278	Pyruvate metabolism, carbon metabolism, phosphotransferase system (PTS), and glycolysis/gluconeogenesis genes were overexpressed.
29867910	9	31	theme	sequencing	1001:1010	arg1	analysis					1012:1019	LAB transcriptome sequencing analysis	983:1019	LAB transcriptome sequencing analysis	983:1019	LAB transcriptome sequencing analysis was performed to elucidate the mechanism responsible for the proliferative effect of LBP.
29867910	1	32	theme	Lycium	105:110	arg1	barbarum					112:119	Lycium barbarum	105:119	Lycium barbarum	105:119	Lycium barbarum is a boxthorn that produces the goji berries.
29867910	1	32	theme	Lycium	105:110	arg1	boxthorn					126:133	a boxthorn	124:133	a boxthorn that produces the goji berries	124:164	Lycium barbarum is a boxthorn that produces the goji berries.
29867910	7	33	theme	lactate	739:745	arg1	LDH					762:764	LDH	762:764	LDH	762:764	In the presence of LBP in the growth medium, the β-galactosidase (β-GAL) and lactate dehydrogenase (LDH) activities of strain Bi-26 significantly increased.
29867910	7	33	theme	lactate	739:745	arg1	dehydrogenase					747:759	lactate dehydrogenase	739:759	lactate dehydrogenase (LDH)	739:765	In the presence of LBP in the growth medium, the β-galactosidase (β-GAL) and lactate dehydrogenase (LDH) activities of strain Bi-26 significantly increased.
29867910	8	34	theme	LDH	844:846	arg1	activities					823:832	The activities	819:832	The activities of β-GAL, LDH, hexokinase (HK), 6-phosphofructokinase (PFK), and pyruvate kinase (PK) of strain NCFM	819:933	The activities of β-GAL, LDH, hexokinase (HK), 6-phosphofructokinase (PFK), and pyruvate kinase (PK) of strain NCFM significantly increased under those conditions.
29867910	5	35	dep	longum	597:602	arg1	subsp					604:608	subsp	604:608	Bifidobacterium longum subsp	581:608	LBP obviously promoted the proliferation of lactic acid bacteria (LAB) strains, especially Bifidobacterium longum subsp.
29867910	12	36	theme	cell	1416:1419	arg1	vitality					1421:1428	cell vitality	1416:1428	cell vitality	1416:1428	Furthermore, LBP improved cell vitality during freeze-drying and tolerance of the gastrointestinal environment.
29867910	11	37	theme	phosphotransferase	1300:1317	arg1	PTS					1327:1329	PTS	1327:1329	PTS	1327:1329	Pyruvate metabolism, carbon metabolism, phosphotransferase system (PTS), and glycolysis/gluconeogenesis genes were overexpressed.
29867910	11	37	theme	phosphotransferase	1300:1317	arg1	system					1319:1324	phosphotransferase system	1300:1324	phosphotransferase system (PTS)	1300:1330	Pyruvate metabolism, carbon metabolism, phosphotransferase system (PTS), and glycolysis/gluconeogenesis genes were overexpressed.
29867910	12	38	theme	gastrointestinal	1472:1487	arg1	environment					1489:1499	the gastrointestinal environment	1468:1499	the gastrointestinal environment	1468:1499	Furthermore, LBP improved cell vitality during freeze-drying and tolerance of the gastrointestinal environment.
29867910	4	39	contain	contained	418:426	arg2	rhamnose					439:446	rhamnose	439:446	rhamnose	439:446	The LBP extract contained arabinose, rhamnose, xylose, mannose, galactose, and glucose.
29867910	4	39	contain	contained	418:426	arg2	glucose					481:487	glucose	481:487	glucose	481:487	The LBP extract contained arabinose, rhamnose, xylose, mannose, galactose, and glucose.
29867910	4	39	contain	contained	418:426	arg2	xylose					449:454	xylose	449:454	xylose	449:454	The LBP extract contained arabinose, rhamnose, xylose, mannose, galactose, and glucose.
29867910	4	39	contain	contained	418:426	arg2	arabinose					428:436	arabinose	428:436	arabinose	428:436	The LBP extract contained arabinose, rhamnose, xylose, mannose, galactose, and glucose.
29867910	4	39	contain	contained	418:426	arg2	mannose					457:463	mannose	457:463	mannose	457:463	The LBP extract contained arabinose, rhamnose, xylose, mannose, galactose, and glucose.
29867910	4	39	contain	contained	418:426	arg2	galactose					466:474	galactose	466:474	galactose	466:474	The LBP extract contained arabinose, rhamnose, xylose, mannose, galactose, and glucose.
29867910	4	39	contain	contained	418:426	arg1	extract					410:416	The LBP extract	402:416	The LBP extract	402:416	The LBP extract contained arabinose, rhamnose, xylose, mannose, galactose, and glucose.
29867910	3	40	theme	gas	377:379	arg1	chromatography					381:394	gas chromatography	377:394	gas chromatography (GC)	377:399	LBP was extracted from goji berries and its monosaccharide composition characterized by gas chromatography (GC).
29867910	3	40	theme	gas	377:379	arg1	GC					397:398	GC	397:398	GC	397:398	LBP was extracted from goji berries and its monosaccharide composition characterized by gas chromatography (GC).
29867910	0	41	theme	Prebiotics	45:54	arg1	Candidate					56:64	A Novel Prebiotics Candidate	37:64	Lyciumbarbarum Polysaccharide (LBP): A Novel Prebiotics Candidate for Bifidobacterium and Lactobacillus.	0:103	Lyciumbarbarum Polysaccharide (LBP): A Novel Prebiotics Candidate for Bifidobacterium and Lactobacillus.
29867910	8	42	theme	hexokinase	849:858	arg1	activities					823:832	The activities	819:832	The activities of β-GAL, LDH, hexokinase (HK), 6-phosphofructokinase (PFK), and pyruvate kinase (PK) of strain NCFM	819:933	The activities of β-GAL, LDH, hexokinase (HK), 6-phosphofructokinase (PFK), and pyruvate kinase (PK) of strain NCFM significantly increased under those conditions.
29867910	7	43	theme	β-galactosidase	711:725	arg1	activities					767:776	the β-galactosidase (β-GAL) and lactate dehydrogenase (LDH) activities	707:776	the β-galactosidase (β-GAL) and lactate dehydrogenase (LDH) activities of strain Bi-26	707:792	In the presence of LBP in the growth medium, the β-galactosidase (β-GAL) and lactate dehydrogenase (LDH) activities of strain Bi-26 significantly increased.
29867910	2	44	theme	study	190:194	arg1	aim					171:173	The aim	167:173	The aim of the current study	167:194	The aim of the current study was to evaluate the proliferative effect of L. barbarum polysaccharides (LBP) on probiotics.
29867910	2	45	theme	current	182:188	arg1	study					190:194	the current study	178:194	the current study	178:194	The aim of the current study was to evaluate the proliferative effect of L. barbarum polysaccharides (LBP) on probiotics.
29867910	2	46	theme	L.	240:241	arg1	LBP					269:271	LBP	269:271	LBP	269:271	The aim of the current study was to evaluate the proliferative effect of L. barbarum polysaccharides (LBP) on probiotics.
29867910	2	46	theme	L.	240:241	arg1	polysaccharides					252:266	L. barbarum polysaccharides	240:266	L. barbarum polysaccharides (LBP)	240:272	The aim of the current study was to evaluate the proliferative effect of L. barbarum polysaccharides (LBP) on probiotics.
29867910	9	47	theme	LBP	1106:1108	arg1	effect					1096:1101	the proliferative effect	1078:1101	the proliferative effect of LBP	1078:1108	LAB transcriptome sequencing analysis was performed to elucidate the mechanism responsible for the proliferative effect of LBP.
29867910	9	48	theme	responsible	1062:1072	arg1	mechanism					1052:1060	the mechanism	1048:1060	the mechanism responsible for the proliferative effect of LBP	1048:1108	LAB transcriptome sequencing analysis was performed to elucidate the mechanism responsible for the proliferative effect of LBP.
29867910	4	49	theme	LBP	406:408	arg1	extract					410:416	The LBP extract	402:416	The LBP extract	402:416	The LBP extract contained arabinose, rhamnose, xylose, mannose, galactose, and glucose.
29867910	10	50	theme	gene	1199:1202	arg1	expression					1204:1213	gene expression	1199:1213	gene expression	1199:1213	The data revealed that LBP promoted the bacterial biosynthetic and metabolic processes, gene expression, transcription, and transmembrane transport.
29867910	8	51	theme	6-phosphofructokinase	866:886	arg1	activities					823:832	The activities	819:832	The activities of β-GAL, LDH, hexokinase (HK), 6-phosphofructokinase (PFK), and pyruvate kinase (PK) of strain NCFM	819:933	The activities of β-GAL, LDH, hexokinase (HK), 6-phosphofructokinase (PFK), and pyruvate kinase (PK) of strain NCFM significantly increased under those conditions.
29867910	9	52	theme	transcriptome	987:999	arg1	analysis					1012:1019	LAB transcriptome sequencing analysis	983:1019	LAB transcriptome sequencing analysis	983:1019	LAB transcriptome sequencing analysis was performed to elucidate the mechanism responsible for the proliferative effect of LBP.
29867910	0	53	dep	Polysaccharide	15:28	arg1	Candidate					56:64	A Novel Prebiotics Candidate	37:64	Lyciumbarbarum Polysaccharide (LBP): A Novel Prebiotics Candidate for Bifidobacterium and Lactobacillus.	0:103	Lyciumbarbarum Polysaccharide (LBP): A Novel Prebiotics Candidate for Bifidobacterium and Lactobacillus.
29867910	13	54	used	used	1525:1528	arg2	LBP					1514:1516	LBP	1514:1516	LBP	1514:1516	In summary, LBP can be used as a potential prebiotic for Bifidobacterium and Lactobacillus.
29867910	13	54	used	used	1525:1528	arg2	prebiotic					1545:1553	a potential prebiotic	1533:1553	a potential prebiotic for Bifidobacterium and Lactobacillus	1533:1591	In summary, LBP can be used as a potential prebiotic for Bifidobacterium and Lactobacillus.
29867910	7	55	theme	growth	692:697	arg1	medium					699:704	the growth medium	688:704	the growth medium	688:704	In the presence of LBP in the growth medium, the β-galactosidase (β-GAL) and lactate dehydrogenase (LDH) activities of strain Bi-26 significantly increased.
29867910	0	56	theme	Novel	39:43	arg1	Candidate					56:64	A Novel Prebiotics Candidate	37:64	Lyciumbarbarum Polysaccharide (LBP): A Novel Prebiotics Candidate for Bifidobacterium and Lactobacillus.	0:103	Lyciumbarbarum Polysaccharide (LBP): A Novel Prebiotics Candidate for Bifidobacterium and Lactobacillus.
29867910	2	57	theme	proliferative	216:228	arg1	effect					230:235	the proliferative effect	212:235	the proliferative effect of L. barbarum polysaccharides (LBP) on probiotics	212:286	The aim of the current study was to evaluate the proliferative effect of L. barbarum polysaccharides (LBP) on probiotics.
29867910	9	58	theme	proliferative	1082:1094	arg1	effect					1096:1101	the proliferative effect	1078:1101	the proliferative effect of LBP	1078:1108	LAB transcriptome sequencing analysis was performed to elucidate the mechanism responsible for the proliferative effect of LBP.
30510995	11	0	dep	Conclusions	1593:1603	arg1	suggest					1619:1625	suggest	1619:1625	suggest that skin inflammation may contribute to pathogenic conditions in the gut via immunologic and microbiological changes	1619:1743	Conclusions These results suggest that skin inflammation may contribute to pathogenic conditions in the gut via immunologic and microbiological changes.
30510995	8	1	theme	cells	1229:1233	arg1	numbers					1249:1255	increased numbers	1239:1255	increased numbers of non-cytokine-producing macrophages in the gut	1239:1304	IMQ mice had decreased numbers of IgD+ and IgM+ B cells and increased numbers of non-cytokine-producing macrophages in the gut.
30510995	8	1	theme	cells	1229:1233	arg1	numbers					1202:1208	decreased numbers	1192:1208	decreased numbers of IgD+ and IgM+ B cells	1192:1233	IMQ mice had decreased numbers of IgD+ and IgM+ B cells and increased numbers of non-cytokine-producing macrophages in the gut.
30510995	3	2	theme	sodium	680:685	arg1	DSS					688:690	fed dextran sulfate sodium (DSS)	660:691	fed dextran sulfate sodium (DSS)	660:691	To study this connection, we used a combination murine model of psoriatic dermatitis and colitis in which mice were treated topically with the Toll-like receptor 7 agonist imiquimod (IMQ) and fed dextran sulfate sodium (DSS).
30510995	3	3	theme	murine	516:521	arg1	model					523:527	a combination murine model	502:527	a combination murine model of psoriatic dermatitis and colitis in which mice were treated topically with the Toll-like receptor 7 agonist imiquimod (IMQ) and fed dextran sulfate sodium (DSS)	502:691	To study this connection, we used a combination murine model of psoriatic dermatitis and colitis in which mice were treated topically with the Toll-like receptor 7 agonist imiquimod (IMQ) and fed dextran sulfate sodium (DSS).
30510995	8	4	from	macrophages	1283:1293	arg1	gut					1302:1304	the gut	1298:1304	the gut	1298:1304	IMQ mice had decreased numbers of IgD+ and IgM+ B cells and increased numbers of non-cytokine-producing macrophages in the gut.
30510995	11	5	from	conditions	1679:1688	arg1	gut					1697:1699	the gut	1693:1699	the gut	1693:1699	Conclusions These results suggest that skin inflammation may contribute to pathogenic conditions in the gut via immunologic and microbiological changes.
30510995	3	6	theme	dextran	664:670	arg1	DSS					688:690	fed dextran sulfate sodium (DSS)	660:691	fed dextran sulfate sodium (DSS)	660:691	To study this connection, we used a combination murine model of psoriatic dermatitis and colitis in which mice were treated topically with the Toll-like receptor 7 agonist imiquimod (IMQ) and fed dextran sulfate sodium (DSS).
30510995	1	7	theme	inflammatory	174:185	arg1	IBD					202:204	IBD	202:204	IBD	202:204	Background & Aims Psoriasis and inflammatory bowel disease (IBD) are both chronic inflammatory diseases occurring in the skin and gut, respectively.
30510995	1	7	theme	inflammatory	174:185	arg1	disease					193:199	inflammatory bowel disease	174:199	inflammatory bowel disease (IBD)	174:205	Background & Aims Psoriasis and inflammatory bowel disease (IBD) are both chronic inflammatory diseases occurring in the skin and gut, respectively.
30510995	1	7	theme	inflammatory	174:185	arg1	Aims					155:158	Background & Aims	142:158	Background & Aims Psoriasis and inflammatory bowel disease (IBD)	142:205	Background & Aims Psoriasis and inflammatory bowel disease (IBD) are both chronic inflammatory diseases occurring in the skin and gut, respectively.
30510995	3	8	theme	dermatitis	542:551	arg1	model					523:527	a combination murine model	502:527	a combination murine model of psoriatic dermatitis and colitis in which mice were treated topically with the Toll-like receptor 7 agonist imiquimod (IMQ) and fed dextran sulfate sodium (DSS)	502:691	To study this connection, we used a combination murine model of psoriatic dermatitis and colitis in which mice were treated topically with the Toll-like receptor 7 agonist imiquimod (IMQ) and fed dextran sulfate sodium (DSS).
30510995	9	9	theme	gut	1321:1323	arg1	microbiomes					1325:1335	the gut microbiomes	1317:1335	the gut microbiomes of IMQ mice	1317:1347	Moreover, the gut microbiomes of IMQ mice were perturbed, with significant reductions of Lactobacillus johnsonii and Lactobacillus reuteri populations.
30510995	8	10	theme	B	1227:1227	arg1	cells					1229:1233	IgD+ and IgM+ B cells	1213:1233	IgD+ and IgM+ B cells	1213:1233	IMQ mice had decreased numbers of IgD+ and IgM+ B cells and increased numbers of non-cytokine-producing macrophages in the gut.
30510995	12	11	theme	psoriasis	1846:1854	arg1	coincidence					1831:1841	the coincidence	1827:1841	the coincidence of psoriasis and IBD	1827:1862	Our finding of a novel potential skin-gut interaction provides new insights into the coincidence of psoriasis and IBD.
30510995	9	12	theme	mice	1344:1347	arg1	microbiomes					1325:1335	the gut microbiomes	1317:1335	the gut microbiomes of IMQ mice	1317:1347	Moreover, the gut microbiomes of IMQ mice were perturbed, with significant reductions of Lactobacillus johnsonii and Lactobacillus reuteri populations.
30510995	12	13	theme	IBD	1860:1862	arg1	coincidence					1831:1841	the coincidence	1827:1841	the coincidence of psoriasis and IBD	1827:1862	Our finding of a novel potential skin-gut interaction provides new insights into the coincidence of psoriasis and IBD.
30510995	9	14	dep	Lactobacillus	1424:1436	arg1	reuteri					1438:1444	reuteri	1438:1444	reuteri	1438:1444	Moreover, the gut microbiomes of IMQ mice were perturbed, with significant reductions of Lactobacillus johnsonii and Lactobacillus reuteri populations.
30510995	10	15	theme	IMQ	1503:1505	arg1	mice					1507:1510	IMQ mice	1503:1510	IMQ mice	1503:1510	Germ-free mice transplanted with feces from IMQ mice, but not with feces from untreated mice, also developed exacerbated DSS colitis.
30510995	5	16	theme	RNA	950:952	arg1	sequencing					954:963	16S ribosomal RNA sequencing	936:963	16S ribosomal RNA sequencing	936:963	Disease activity and immune cell phenotypes were analyzed, and the microbial composition of fecal samples was investigated using 16S ribosomal RNA sequencing.
30510995	3	17	theme	colitis	557:563	arg1	model					523:527	a combination murine model	502:527	a combination murine model of psoriatic dermatitis and colitis in which mice were treated topically with the Toll-like receptor 7 agonist imiquimod (IMQ) and fed dextran sulfate sodium (DSS)	502:691	To study this connection, we used a combination murine model of psoriatic dermatitis and colitis in which mice were treated topically with the Toll-like receptor 7 agonist imiquimod (IMQ) and fed dextran sulfate sodium (DSS).
30510995	1	18	dep	skin	263:266	arg1	the					259:261	the	259:261	the	259:261	Background & Aims Psoriasis and inflammatory bowel disease (IBD) are both chronic inflammatory diseases occurring in the skin and gut, respectively.
30510995	6	19	theme	microbiome	1083:1092	arg1	effect					1065:1070	the effect	1061:1070	the effect of the gut microbiome on disease	1061:1103	We transplanted feces from IMQ mice to germ-free IQI/Jic (IQI) mice and fed them DSS to assess the effect of the gut microbiome on disease.
30510995	8	20	theme	IMQ	1179:1181	arg1	mice					1183:1186	IMQ mice	1179:1186	IMQ mice	1179:1186	IMQ mice had decreased numbers of IgD+ and IgM+ B cells and increased numbers of non-cytokine-producing macrophages in the gut.
30510995	11	21	theme	skin	1632:1635	arg1	inflammation					1637:1648	skin inflammation	1632:1648	skin inflammation	1632:1648	Conclusions These results suggest that skin inflammation may contribute to pathogenic conditions in the gut via immunologic and microbiological changes.
30510995	5	22	theme	cell	835:838	arg1	phenotypes					840:849	immune cell phenotypes	828:849	immune cell phenotypes	828:849	Disease activity and immune cell phenotypes were analyzed, and the microbial composition of fecal samples was investigated using 16S ribosomal RNA sequencing.
30510995	0	23	theme	Sulfate	70:76	arg1	Colitis					85:91	Severe Dextran Sulfate Sodium Colitis	55:91	Severe Dextran Sulfate Sodium Colitis	55:91	Toll-Like Receptor 7 Agonist-Induced Dermatitis Causes Severe Dextran Sulfate Sodium Colitis by Altering the Gut Microbiome and Immune Cells.
30510995	1	24	theme	&	153:153	arg1	disease					193:199	inflammatory bowel disease	174:199	inflammatory bowel disease (IBD)	174:205	Background & Aims Psoriasis and inflammatory bowel disease (IBD) are both chronic inflammatory diseases occurring in the skin and gut, respectively.
30510995	1	24	theme	&	153:153	arg1	diseases					237:244	both chronic inflammatory diseases	211:244	both chronic inflammatory diseases occurring in the skin and gut, respectively	211:288	Background & Aims Psoriasis and inflammatory bowel disease (IBD) are both chronic inflammatory diseases occurring in the skin and gut, respectively.
30510995	1	24	theme	&	153:153	arg1	Aims					155:158	Background & Aims	142:158	Background & Aims Psoriasis and inflammatory bowel disease (IBD)	142:205	Background & Aims Psoriasis and inflammatory bowel disease (IBD) are both chronic inflammatory diseases occurring in the skin and gut, respectively.
30510995	1	24	theme	&	153:153	arg1	Psoriasis					160:168	Psoriasis	160:168	Psoriasis	160:168	Background & Aims Psoriasis and inflammatory bowel disease (IBD) are both chronic inflammatory diseases occurring in the skin and gut, respectively.
30510995	10	25	theme	Germ-free	1459:1467	arg1	mice					1469:1472	Germ-free mice	1459:1472	Germ-free mice	1459:1472	Germ-free mice transplanted with feces from IMQ mice, but not with feces from untreated mice, also developed exacerbated DSS colitis.
30510995	4	26	from	DSS	778:780	arg1	water					800:804	their drinking water	785:804	their drinking water	785:804	Methods We applied IMQ topically to B6 mice (IMQ mice) and subsequently fed them 2% DSS in their drinking water.
30510995	5	27	theme	Disease	807:813	arg1	activity					815:822	Disease activity	807:822	Disease activity	807:822	Disease activity and immune cell phenotypes were analyzed, and the microbial composition of fecal samples was investigated using 16S ribosomal RNA sequencing.
30510995	8	28	theme	IgD+	1213:1216	arg1	cells					1229:1233	IgD+ and IgM+ B cells	1213:1233	IgD+ and IgM+ B cells	1213:1233	IMQ mice had decreased numbers of IgD+ and IgM+ B cells and increased numbers of non-cytokine-producing macrophages in the gut.
30510995	8	29	theme	IgM+	1222:1225	arg1	cells					1229:1233	IgD+ and IgM+ B cells	1213:1233	IgD+ and IgM+ B cells	1213:1233	IMQ mice had decreased numbers of IgD+ and IgM+ B cells and increased numbers of non-cytokine-producing macrophages in the gut.
30510995	2	30	theme	similar	370:376	arg1	changes					378:384	similar changes	370:384	similar changes in immune cells and microbiome composition	370:427	It is well established that psoriasis and IBD have high concordance rates, and similar changes in immune cells and microbiome composition have been reported in both conditions.
30510995	0	31	theme	Gut	109:111	arg1	Microbiome					113:122	the Gut Microbiome	105:122	the Gut Microbiome	105:122	Toll-Like Receptor 7 Agonist-Induced Dermatitis Causes Severe Dextran Sulfate Sodium Colitis by Altering the Gut Microbiome and Immune Cells.
30510995	12	32	theme	skin-gut	1779:1786	arg1	interaction					1788:1798	a novel potential skin-gut interaction	1761:1798	a novel potential skin-gut interaction	1761:1798	Our finding of a novel potential skin-gut interaction provides new insights into the coincidence of psoriasis and IBD.
30510995	8	33	from	numbers	1202:1208	arg1	gut					1302:1304	the gut	1298:1304	the gut	1298:1304	IMQ mice had decreased numbers of IgD+ and IgM+ B cells and increased numbers of non-cytokine-producing macrophages in the gut.
30510995	1	34	dep	Aims	155:158	arg1	IBD					202:204	IBD	202:204	IBD	202:204	Background & Aims Psoriasis and inflammatory bowel disease (IBD) are both chronic inflammatory diseases occurring in the skin and gut, respectively.
30510995	1	34	dep	Aims	155:158	arg1	disease					193:199	inflammatory bowel disease	174:199	inflammatory bowel disease (IBD)	174:205	Background & Aims Psoriasis and inflammatory bowel disease (IBD) are both chronic inflammatory diseases occurring in the skin and gut, respectively.
30510995	1	34	dep	Aims	155:158	arg1	Aims					155:158	Background & Aims	142:158	Background & Aims Psoriasis and inflammatory bowel disease (IBD)	142:205	Background & Aims Psoriasis and inflammatory bowel disease (IBD) are both chronic inflammatory diseases occurring in the skin and gut, respectively.
30510995	1	34	dep	Aims	155:158	arg1	Psoriasis					160:168	Psoriasis	160:168	Psoriasis	160:168	Background & Aims Psoriasis and inflammatory bowel disease (IBD) are both chronic inflammatory diseases occurring in the skin and gut, respectively.
30510995	0	35	theme	Toll-Like	0:8	arg1	Receptor					10:17	Toll-Like Receptor 7	0:19	Toll-Like Receptor 7 Agonist-Induced Dermatitis	0:46	Toll-Like Receptor 7 Agonist-Induced Dermatitis Causes Severe Dextran Sulfate Sodium Colitis by Altering the Gut Microbiome and Immune Cells.
30510995	2	36	theme	microbiome	406:415	arg1	composition					417:427	microbiome composition	406:427	microbiome composition	406:427	It is well established that psoriasis and IBD have high concordance rates, and similar changes in immune cells and microbiome composition have been reported in both conditions.
30510995	8	37	theme	increased	1239:1247	arg1	numbers					1249:1255	increased numbers	1239:1255	increased numbers of non-cytokine-producing macrophages in the gut	1239:1304	IMQ mice had decreased numbers of IgD+ and IgM+ B cells and increased numbers of non-cytokine-producing macrophages in the gut.
30510995	9	38	theme	significant	1370:1380	arg1	reductions					1382:1391	significant reductions	1370:1391	significant reductions of Lactobacillus johnsonii and Lactobacillus reuteri populations	1370:1456	Moreover, the gut microbiomes of IMQ mice were perturbed, with significant reductions of Lactobacillus johnsonii and Lactobacillus reuteri populations.
30510995	3	39	theme	receptor	621:628	arg1	IMQ					651:653	IMQ	651:653	IMQ	651:653	To study this connection, we used a combination murine model of psoriatic dermatitis and colitis in which mice were treated topically with the Toll-like receptor 7 agonist imiquimod (IMQ) and fed dextran sulfate sodium (DSS).
30510995	3	39	theme	receptor	621:628	arg1	imiquimod					640:648	the Toll-like receptor 7 agonist imiquimod	607:648	the Toll-like receptor 7 agonist imiquimod (IMQ)	607:654	To study this connection, we used a combination murine model of psoriatic dermatitis and colitis in which mice were treated topically with the Toll-like receptor 7 agonist imiquimod (IMQ) and fed dextran sulfate sodium (DSS).
30510995	12	40	theme	novel	1763:1767	arg1	interaction					1788:1798	a novel potential skin-gut interaction	1761:1798	a novel potential skin-gut interaction	1761:1798	Our finding of a novel potential skin-gut interaction provides new insights into the coincidence of psoriasis and IBD.
30510995	8	41	theme	macrophages	1283:1293	arg1	numbers					1249:1255	increased numbers	1239:1255	increased numbers of non-cytokine-producing macrophages in the gut	1239:1304	IMQ mice had decreased numbers of IgD+ and IgM+ B cells and increased numbers of non-cytokine-producing macrophages in the gut.
30510995	8	41	theme	macrophages	1283:1293	arg1	numbers					1202:1208	decreased numbers	1192:1208	decreased numbers of IgD+ and IgM+ B cells	1192:1233	IMQ mice had decreased numbers of IgD+ and IgM+ B cells and increased numbers of non-cytokine-producing macrophages in the gut.
30510995	0	42	theme	Severe	55:60	arg1	Colitis					85:91	Severe Dextran Sulfate Sodium Colitis	55:91	Severe Dextran Sulfate Sodium Colitis	55:91	Toll-Like Receptor 7 Agonist-Induced Dermatitis Causes Severe Dextran Sulfate Sodium Colitis by Altering the Gut Microbiome and Immune Cells.
30510995	5	43	theme	ribosomal	940:948	arg1	sequencing					954:963	16S ribosomal RNA sequencing	936:963	16S ribosomal RNA sequencing	936:963	Disease activity and immune cell phenotypes were analyzed, and the microbial composition of fecal samples was investigated using 16S ribosomal RNA sequencing.
30510995	7	44	theme	accelerated	1154:1164	arg1	colitis					1170:1176	accelerated DSS colitis	1154:1176	accelerated DSS colitis	1154:1176	Results We first confirmed that IMQ mice showed accelerated DSS colitis.
30510995	4	45	theme	B6	730:731	arg1	mice					733:736	B6 mice	730:736	B6 mice (IMQ mice)	730:747	Methods We applied IMQ topically to B6 mice (IMQ mice) and subsequently fed them 2% DSS in their drinking water.
30510995	4	45	theme	B6	730:731	arg1	mice					743:746	IMQ mice	739:746	IMQ mice	739:746	Methods We applied IMQ topically to B6 mice (IMQ mice) and subsequently fed them 2% DSS in their drinking water.
30510995	10	46	theme	exacerbated	1568:1578	arg1	colitis					1584:1590	exacerbated DSS colitis	1568:1590	exacerbated DSS colitis	1568:1590	Germ-free mice transplanted with feces from IMQ mice, but not with feces from untreated mice, also developed exacerbated DSS colitis.
30510995	5	47	theme	fecal	899:903	arg1	samples					905:911	fecal samples	899:911	fecal samples	899:911	Disease activity and immune cell phenotypes were analyzed, and the microbial composition of fecal samples was investigated using 16S ribosomal RNA sequencing.
30510995	10	48	from	mice	1547:1550	arg1	feces					1526:1530	feces	1526:1530	feces from untreated mice	1526:1550	Germ-free mice transplanted with feces from IMQ mice, but not with feces from untreated mice, also developed exacerbated DSS colitis.
30510995	3	49	theme	agonist	632:638	arg1	IMQ					651:653	IMQ	651:653	IMQ	651:653	To study this connection, we used a combination murine model of psoriatic dermatitis and colitis in which mice were treated topically with the Toll-like receptor 7 agonist imiquimod (IMQ) and fed dextran sulfate sodium (DSS).
30510995	3	49	theme	agonist	632:638	arg1	imiquimod					640:648	the Toll-like receptor 7 agonist imiquimod	607:648	the Toll-like receptor 7 agonist imiquimod (IMQ)	607:654	To study this connection, we used a combination murine model of psoriatic dermatitis and colitis in which mice were treated topically with the Toll-like receptor 7 agonist imiquimod (IMQ) and fed dextran sulfate sodium (DSS).
30510995	6	50	from	mice	997:1000	arg1	feces					982:986	feces	982:986	feces from IMQ mice to germ-free IQI/Jic (IQI) mice	982:1032	We transplanted feces from IMQ mice to germ-free IQI/Jic (IQI) mice and fed them DSS to assess the effect of the gut microbiome on disease.
30510995	2	51	theme	concordance	347:357	arg1	rates					359:363	high concordance rates	342:363	high concordance rates	342:363	It is well established that psoriasis and IBD have high concordance rates, and similar changes in immune cells and microbiome composition have been reported in both conditions.
30510995	1	52	theme	inflammatory	224:235	arg1	diseases					237:244	both chronic inflammatory diseases	211:244	both chronic inflammatory diseases occurring in the skin and gut, respectively	211:288	Background & Aims Psoriasis and inflammatory bowel disease (IBD) are both chronic inflammatory diseases occurring in the skin and gut, respectively.
30510995	1	52	theme	inflammatory	224:235	arg1	Aims					155:158	Background & Aims	142:158	Background & Aims Psoriasis and inflammatory bowel disease (IBD)	142:205	Background & Aims Psoriasis and inflammatory bowel disease (IBD) are both chronic inflammatory diseases occurring in the skin and gut, respectively.
30510995	6	53	theme	germ-free	1005:1013	arg1	mice					1029:1032	germ-free IQI/Jic (IQI) mice	1005:1032	germ-free IQI/Jic (IQI) mice	1005:1032	We transplanted feces from IMQ mice to germ-free IQI/Jic (IQI) mice and fed them DSS to assess the effect of the gut microbiome on disease.
30510995	3	54	theme	fed	660:662	arg1	DSS					688:690	fed dextran sulfate sodium (DSS)	660:691	fed dextran sulfate sodium (DSS)	660:691	To study this connection, we used a combination murine model of psoriatic dermatitis and colitis in which mice were treated topically with the Toll-like receptor 7 agonist imiquimod (IMQ) and fed dextran sulfate sodium (DSS).
30510995	4	55	theme	%	776:776	arg1	DSS					778:780	2% DSS	775:780	2% DSS in their drinking water	775:804	Methods We applied IMQ topically to B6 mice (IMQ mice) and subsequently fed them 2% DSS in their drinking water.
30510995	3	56	theme	combination	504:514	arg1	model					523:527	a combination murine model	502:527	a combination murine model of psoriatic dermatitis and colitis in which mice were treated topically with the Toll-like receptor 7 agonist imiquimod (IMQ) and fed dextran sulfate sodium (DSS)	502:691	To study this connection, we used a combination murine model of psoriatic dermatitis and colitis in which mice were treated topically with the Toll-like receptor 7 agonist imiquimod (IMQ) and fed dextran sulfate sodium (DSS).
30510995	10	57	from	mice	1507:1510	arg1	feces					1492:1496	feces	1492:1496	feces from IMQ mice	1492:1510	Germ-free mice transplanted with feces from IMQ mice, but not with feces from untreated mice, also developed exacerbated DSS colitis.
30510995	3	58	used	used	497:500	arg2	we					494:495	we	494:495	we	494:495	To study this connection, we used a combination murine model of psoriatic dermatitis and colitis in which mice were treated topically with the Toll-like receptor 7 agonist imiquimod (IMQ) and fed dextran sulfate sodium (DSS).
30510995	3	59	theme	sulfate	672:678	arg1	DSS					688:690	fed dextran sulfate sodium (DSS)	660:691	fed dextran sulfate sodium (DSS)	660:691	To study this connection, we used a combination murine model of psoriatic dermatitis and colitis in which mice were treated topically with the Toll-like receptor 7 agonist imiquimod (IMQ) and fed dextran sulfate sodium (DSS).
30510995	3	60	theme	psoriatic	532:540	arg1	dermatitis					542:551	psoriatic dermatitis	532:551	psoriatic dermatitis	532:551	To study this connection, we used a combination murine model of psoriatic dermatitis and colitis in which mice were treated topically with the Toll-like receptor 7 agonist imiquimod (IMQ) and fed dextran sulfate sodium (DSS).
30510995	8	61	from	numbers	1249:1255	arg1	gut					1302:1304	the gut	1298:1304	the gut	1298:1304	IMQ mice had decreased numbers of IgD+ and IgM+ B cells and increased numbers of non-cytokine-producing macrophages in the gut.
30510995	1	62	theme	bowel	187:191	arg1	IBD					202:204	IBD	202:204	IBD	202:204	Background & Aims Psoriasis and inflammatory bowel disease (IBD) are both chronic inflammatory diseases occurring in the skin and gut, respectively.
30510995	1	62	theme	bowel	187:191	arg1	disease					193:199	inflammatory bowel disease	174:199	inflammatory bowel disease (IBD)	174:205	Background & Aims Psoriasis and inflammatory bowel disease (IBD) are both chronic inflammatory diseases occurring in the skin and gut, respectively.
30510995	1	62	theme	bowel	187:191	arg1	Aims					155:158	Background & Aims	142:158	Background & Aims Psoriasis and inflammatory bowel disease (IBD)	142:205	Background & Aims Psoriasis and inflammatory bowel disease (IBD) are both chronic inflammatory diseases occurring in the skin and gut, respectively.
30510995	0	63	theme	Immune	128:133	arg1	Cells					135:139	Immune Cells	128:139	Immune Cells	128:139	Toll-Like Receptor 7 Agonist-Induced Dermatitis Causes Severe Dextran Sulfate Sodium Colitis by Altering the Gut Microbiome and Immune Cells.
30510995	8	64	from	gut	1302:1304	arg1	numbers					1249:1255	increased numbers	1239:1255	increased numbers of non-cytokine-producing macrophages in the gut	1239:1304	IMQ mice had decreased numbers of IgD+ and IgM+ B cells and increased numbers of non-cytokine-producing macrophages in the gut.
30510995	8	64	from	gut	1302:1304	arg1	numbers					1202:1208	decreased numbers	1192:1208	decreased numbers of IgD+ and IgM+ B cells	1192:1233	IMQ mice had decreased numbers of IgD+ and IgM+ B cells and increased numbers of non-cytokine-producing macrophages in the gut.
30510995	9	65	theme	Lactobacillus	1396:1408	arg1	populations					1446:1456	Lactobacillus johnsonii and Lactobacillus reuteri populations	1396:1456	Lactobacillus johnsonii and Lactobacillus reuteri populations	1396:1456	Moreover, the gut microbiomes of IMQ mice were perturbed, with significant reductions of Lactobacillus johnsonii and Lactobacillus reuteri populations.
30510995	9	66	theme	IMQ	1340:1342	arg1	mice					1344:1347	IMQ mice	1340:1347	IMQ mice	1340:1347	Moreover, the gut microbiomes of IMQ mice were perturbed, with significant reductions of Lactobacillus johnsonii and Lactobacillus reuteri populations.
30510995	8	67	contain	had	1188:1190	arg2	numbers					1202:1208	decreased numbers	1192:1208	decreased numbers of IgD+ and IgM+ B cells	1192:1233	IMQ mice had decreased numbers of IgD+ and IgM+ B cells and increased numbers of non-cytokine-producing macrophages in the gut.
30510995	8	67	contain	had	1188:1190	arg2	numbers					1249:1255	increased numbers	1239:1255	increased numbers of non-cytokine-producing macrophages in the gut	1239:1304	IMQ mice had decreased numbers of IgD+ and IgM+ B cells and increased numbers of non-cytokine-producing macrophages in the gut.
30510995	8	67	contain	had	1188:1190	arg1	mice					1183:1186	IMQ mice	1179:1186	IMQ mice	1179:1186	IMQ mice had decreased numbers of IgD+ and IgM+ B cells and increased numbers of non-cytokine-producing macrophages in the gut.
30510995	11	68	theme	microbiological	1721:1735	arg1	changes					1737:1743	immunologic and microbiological changes	1705:1743	immunologic and microbiological changes	1705:1743	Conclusions These results suggest that skin inflammation may contribute to pathogenic conditions in the gut via immunologic and microbiological changes.
30510995	4	69	dep	Methods	694:700	arg1	applied					705:711	applied	705:711	applied IMQ topically to B6 mice (IMQ mice)	705:747	Methods We applied IMQ topically to B6 mice (IMQ mice) and subsequently fed them 2% DSS in their drinking water.
30510995	4	69	dep	Methods	694:700	arg1	fed					766:768	fed	766:768	subsequently fed them	753:773	Methods We applied IMQ topically to B6 mice (IMQ mice) and subsequently fed them 2% DSS in their drinking water.
30510995	4	70	theme	drinking	791:798	arg1	water					800:804	their drinking water	785:804	their drinking water	785:804	Methods We applied IMQ topically to B6 mice (IMQ mice) and subsequently fed them 2% DSS in their drinking water.
30510995	11	71	theme	immunologic	1705:1715	arg1	changes					1737:1743	immunologic and microbiological changes	1705:1743	immunologic and microbiological changes	1705:1743	Conclusions These results suggest that skin inflammation may contribute to pathogenic conditions in the gut via immunologic and microbiological changes.
30510995	5	72	theme	immune	828:833	arg1	phenotypes					840:849	immune cell phenotypes	828:849	immune cell phenotypes	828:849	Disease activity and immune cell phenotypes were analyzed, and the microbial composition of fecal samples was investigated using 16S ribosomal RNA sequencing.
30510995	6	73	theme	gut	1079:1081	arg1	microbiome					1083:1092	the gut microbiome	1075:1092	the gut microbiome	1075:1092	We transplanted feces from IMQ mice to germ-free IQI/Jic (IQI) mice and fed them DSS to assess the effect of the gut microbiome on disease.
30510995	7	74	theme	IMQ	1138:1140	arg1	mice					1142:1145	IMQ mice	1138:1145	IMQ mice	1138:1145	Results We first confirmed that IMQ mice showed accelerated DSS colitis.
30510995	0	75	theme	Sodium	78:83	arg1	Colitis					85:91	Severe Dextran Sulfate Sodium Colitis	55:91	Severe Dextran Sulfate Sodium Colitis	55:91	Toll-Like Receptor 7 Agonist-Induced Dermatitis Causes Severe Dextran Sulfate Sodium Colitis by Altering the Gut Microbiome and Immune Cells.
30510995	1	76	theme	Background	142:151	arg1	disease					193:199	inflammatory bowel disease	174:199	inflammatory bowel disease (IBD)	174:205	Background & Aims Psoriasis and inflammatory bowel disease (IBD) are both chronic inflammatory diseases occurring in the skin and gut, respectively.
30510995	1	76	theme	Background	142:151	arg1	diseases					237:244	both chronic inflammatory diseases	211:244	both chronic inflammatory diseases occurring in the skin and gut, respectively	211:288	Background & Aims Psoriasis and inflammatory bowel disease (IBD) are both chronic inflammatory diseases occurring in the skin and gut, respectively.
30510995	1	76	theme	Background	142:151	arg1	Aims					155:158	Background & Aims	142:158	Background & Aims Psoriasis and inflammatory bowel disease (IBD)	142:205	Background & Aims Psoriasis and inflammatory bowel disease (IBD) are both chronic inflammatory diseases occurring in the skin and gut, respectively.
30510995	1	76	theme	Background	142:151	arg1	Psoriasis					160:168	Psoriasis	160:168	Psoriasis	160:168	Background & Aims Psoriasis and inflammatory bowel disease (IBD) are both chronic inflammatory diseases occurring in the skin and gut, respectively.
30510995	8	77	theme	decreased	1192:1200	arg1	numbers					1202:1208	decreased numbers	1192:1208	decreased numbers of IgD+ and IgM+ B cells	1192:1233	IMQ mice had decreased numbers of IgD+ and IgM+ B cells and increased numbers of non-cytokine-producing macrophages in the gut.
30510995	11	78	theme	pathogenic	1668:1677	arg1	conditions					1679:1688	pathogenic conditions	1668:1688	pathogenic conditions in the gut	1668:1699	Conclusions These results suggest that skin inflammation may contribute to pathogenic conditions in the gut via immunologic and microbiological changes.
30510995	2	79	contain	have	337:340	arg1	psoriasis					319:327	psoriasis	319:327	psoriasis	319:327	It is well established that psoriasis and IBD have high concordance rates, and similar changes in immune cells and microbiome composition have been reported in both conditions.
30510995	2	79	contain	have	337:340	arg2	rates					359:363	high concordance rates	342:363	high concordance rates	342:363	It is well established that psoriasis and IBD have high concordance rates, and similar changes in immune cells and microbiome composition have been reported in both conditions.
30510995	2	79	contain	have	337:340	arg1	IBD					333:335	IBD	333:335	IBD	333:335	It is well established that psoriasis and IBD have high concordance rates, and similar changes in immune cells and microbiome composition have been reported in both conditions.
30510995	12	80	theme	interaction	1788:1798	arg1	finding					1750:1756	Our finding	1746:1756	Our finding of a novel potential skin-gut interaction	1746:1798	Our finding of a novel potential skin-gut interaction provides new insights into the coincidence of psoriasis and IBD.
30510995	9	81	theme	Lactobacillus	1424:1436	arg1	populations					1446:1456	Lactobacillus johnsonii and Lactobacillus reuteri populations	1396:1456	Lactobacillus johnsonii and Lactobacillus reuteri populations	1396:1456	Moreover, the gut microbiomes of IMQ mice were perturbed, with significant reductions of Lactobacillus johnsonii and Lactobacillus reuteri populations.
30510995	6	82	theme	IMQ	993:995	arg1	mice					997:1000	IMQ mice	993:1000	IMQ mice	993:1000	We transplanted feces from IMQ mice to germ-free IQI/Jic (IQI) mice and fed them DSS to assess the effect of the gut microbiome on disease.
30510995	6	83	from	effect	1065:1070	arg1	disease					1097:1103	disease	1097:1103	disease	1097:1103	We transplanted feces from IMQ mice to germ-free IQI/Jic (IQI) mice and fed them DSS to assess the effect of the gut microbiome on disease.
30510995	0	84	theme	Receptor	10:17	arg1	Dermatitis					37:46	Toll-Like Receptor 7 Agonist-Induced Dermatitis	0:46	Toll-Like Receptor 7 Agonist-Induced Dermatitis	0:46	Toll-Like Receptor 7 Agonist-Induced Dermatitis Causes Severe Dextran Sulfate Sodium Colitis by Altering the Gut Microbiome and Immune Cells.
30510995	12	85	theme	new	1809:1811	arg1	insights					1813:1820	new insights	1809:1820	new insights into the coincidence of psoriasis and IBD	1809:1862	Our finding of a novel potential skin-gut interaction provides new insights into the coincidence of psoriasis and IBD.
30510995	9	86	theme	populations	1446:1456	arg1	reductions					1382:1391	significant reductions	1370:1391	significant reductions of Lactobacillus johnsonii and Lactobacillus reuteri populations	1370:1456	Moreover, the gut microbiomes of IMQ mice were perturbed, with significant reductions of Lactobacillus johnsonii and Lactobacillus reuteri populations.
30510995	9	87	dep	Lactobacillus	1396:1408	arg1	johnsonii					1410:1418	johnsonii	1410:1418	johnsonii	1410:1418	Moreover, the gut microbiomes of IMQ mice were perturbed, with significant reductions of Lactobacillus johnsonii and Lactobacillus reuteri populations.
30510995	0	88	theme	Agonist-Induced	21:35	arg1	Dermatitis					37:46	Toll-Like Receptor 7 Agonist-Induced Dermatitis	0:46	Toll-Like Receptor 7 Agonist-Induced Dermatitis	0:46	Toll-Like Receptor 7 Agonist-Induced Dermatitis Causes Severe Dextran Sulfate Sodium Colitis by Altering the Gut Microbiome and Immune Cells.
30510995	5	89	theme	microbial	874:882	arg1	composition					884:894	the microbial composition	870:894	the microbial composition of fecal samples	870:911	Disease activity and immune cell phenotypes were analyzed, and the microbial composition of fecal samples was investigated using 16S ribosomal RNA sequencing.
30510995	3	90	theme	Toll-like	611:619	arg1	IMQ					651:653	IMQ	651:653	IMQ	651:653	To study this connection, we used a combination murine model of psoriatic dermatitis and colitis in which mice were treated topically with the Toll-like receptor 7 agonist imiquimod (IMQ) and fed dextran sulfate sodium (DSS).
30510995	3	90	theme	Toll-like	611:619	arg1	imiquimod					640:648	the Toll-like receptor 7 agonist imiquimod	607:648	the Toll-like receptor 7 agonist imiquimod (IMQ)	607:654	To study this connection, we used a combination murine model of psoriatic dermatitis and colitis in which mice were treated topically with the Toll-like receptor 7 agonist imiquimod (IMQ) and fed dextran sulfate sodium (DSS).
30510995	2	91	theme	immune	389:394	arg1	cells					396:400	immune cells	389:400	immune cells	389:400	It is well established that psoriasis and IBD have high concordance rates, and similar changes in immune cells and microbiome composition have been reported in both conditions.
30510995	12	92	theme	potential	1769:1777	arg1	interaction					1788:1798	a novel potential skin-gut interaction	1761:1798	a novel potential skin-gut interaction	1761:1798	Our finding of a novel potential skin-gut interaction provides new insights into the coincidence of psoriasis and IBD.
30510995	7	93	theme	DSS	1166:1168	arg1	colitis					1170:1176	accelerated DSS colitis	1154:1176	accelerated DSS colitis	1154:1176	Results We first confirmed that IMQ mice showed accelerated DSS colitis.
30510995	7	94	dep	Results	1106:1112	arg1	We					1114:1115	We	1114:1115	We	1114:1115	Results We first confirmed that IMQ mice showed accelerated DSS colitis.
30510995	4	95	theme	IMQ	739:741	arg1	mice					733:736	B6 mice	730:736	B6 mice (IMQ mice)	730:747	Methods We applied IMQ topically to B6 mice (IMQ mice) and subsequently fed them 2% DSS in their drinking water.
30510995	4	95	theme	IMQ	739:741	arg1	mice					743:746	IMQ mice	739:746	IMQ mice	739:746	Methods We applied IMQ topically to B6 mice (IMQ mice) and subsequently fed them 2% DSS in their drinking water.
30510995	10	96	theme	untreated	1537:1545	arg1	mice					1547:1550	untreated mice	1537:1550	untreated mice	1537:1550	Germ-free mice transplanted with feces from IMQ mice, but not with feces from untreated mice, also developed exacerbated DSS colitis.
30510995	0	97	theme	Dextran	62:68	arg1	Sulfate					70:76	Dextran Sulfate	62:76	Severe Dextran Sulfate Sodium Colitis	55:91	Toll-Like Receptor 7 Agonist-Induced Dermatitis Causes Severe Dextran Sulfate Sodium Colitis by Altering the Gut Microbiome and Immune Cells.
30510995	5	98	theme	16S	936:938	arg1	sequencing					954:963	16S ribosomal RNA sequencing	936:963	16S ribosomal RNA sequencing	936:963	Disease activity and immune cell phenotypes were analyzed, and the microbial composition of fecal samples was investigated using 16S ribosomal RNA sequencing.
30510995	8	99	theme	non-cytokine-producing	1260:1281	arg1	macrophages					1283:1293	non-cytokine-producing macrophages	1260:1293	non-cytokine-producing macrophages in the gut	1260:1304	IMQ mice had decreased numbers of IgD+ and IgM+ B cells and increased numbers of non-cytokine-producing macrophages in the gut.
30510995	10	100	theme	DSS	1580:1582	arg1	colitis					1584:1590	exacerbated DSS colitis	1568:1590	exacerbated DSS colitis	1568:1590	Germ-free mice transplanted with feces from IMQ mice, but not with feces from untreated mice, also developed exacerbated DSS colitis.
30510995	6	101	theme	IQI	1024:1026	arg1	mice					1029:1032	germ-free IQI/Jic (IQI) mice	1005:1032	germ-free IQI/Jic (IQI) mice	1005:1032	We transplanted feces from IMQ mice to germ-free IQI/Jic (IQI) mice and fed them DSS to assess the effect of the gut microbiome on disease.
30510995	1	102	theme	chronic	216:222	arg1	diseases					237:244	both chronic inflammatory diseases	211:244	both chronic inflammatory diseases occurring in the skin and gut, respectively	211:288	Background & Aims Psoriasis and inflammatory bowel disease (IBD) are both chronic inflammatory diseases occurring in the skin and gut, respectively.
30510995	1	102	theme	chronic	216:222	arg1	Aims					155:158	Background & Aims	142:158	Background & Aims Psoriasis and inflammatory bowel disease (IBD)	142:205	Background & Aims Psoriasis and inflammatory bowel disease (IBD) are both chronic inflammatory diseases occurring in the skin and gut, respectively.
30510995	5	103	theme	samples	905:911	arg1	composition					884:894	the microbial composition	870:894	the microbial composition of fecal samples	870:911	Disease activity and immune cell phenotypes were analyzed, and the microbial composition of fecal samples was investigated using 16S ribosomal RNA sequencing.
30510995	2	104	from	changes	378:384	arg1	composition					417:427	microbiome composition	406:427	microbiome composition	406:427	It is well established that psoriasis and IBD have high concordance rates, and similar changes in immune cells and microbiome composition have been reported in both conditions.
30510995	2	104	from	changes	378:384	arg1	cells					396:400	immune cells	389:400	immune cells	389:400	It is well established that psoriasis and IBD have high concordance rates, and similar changes in immune cells and microbiome composition have been reported in both conditions.
30510995	6	105	theme	IQI/Jic	1015:1021	arg1	mice					1029:1032	germ-free IQI/Jic (IQI) mice	1005:1032	germ-free IQI/Jic (IQI) mice	1005:1032	We transplanted feces from IMQ mice to germ-free IQI/Jic (IQI) mice and fed them DSS to assess the effect of the gut microbiome on disease.
30510995	2	106	theme	high	342:345	arg1	rates					359:363	high concordance rates	342:363	high concordance rates	342:363	It is well established that psoriasis and IBD have high concordance rates, and similar changes in immune cells and microbiome composition have been reported in both conditions.
31677408	0	0	theme	quorum	59:64	arg1	sensing					66:72	quorum sensing	59:72	quorum sensing	59:72	Impact of the acetate/oleic acid ratio on the performance, quorum sensing, and microbial community of sequencing batch reactor system.
31677408	1	1	theme	%	249:249	arg1	%					253:253	20%/80%	247:253	20%/80%	247:253	This study comprehensively investigated the impact of acetate/oleic acid ratios (80%/20%, 60%/40%, 40%/60%, and 20%/80%) on sequencing batch reactor (SBR) with respect to the variations in performance, quorum sensing (QS), and microbial community.
31677408	2	2	theme	acid	456:459	arg1	ratios					461:466	the acetate/oleic acid ratios	438:466	the acetate/oleic acid ratios	438:466	Results showed that NH+4-N removal was not affected by the acetate/oleic acid ratios, while the COD, total nitrogen (TN), and PO3-4-P removal was considerably affected.
31677408	1	3	from	variations	310:319	arg1	community					372:380	microbial community	362:380	microbial community	362:380	This study comprehensively investigated the impact of acetate/oleic acid ratios (80%/20%, 60%/40%, 40%/60%, and 20%/80%) on sequencing batch reactor (SBR) with respect to the variations in performance, quorum sensing (QS), and microbial community.
31677408	1	3	from	variations	310:319	arg1	QS					353:354	QS	353:354	QS	353:354	This study comprehensively investigated the impact of acetate/oleic acid ratios (80%/20%, 60%/40%, 40%/60%, and 20%/80%) on sequencing batch reactor (SBR) with respect to the variations in performance, quorum sensing (QS), and microbial community.
31677408	1	3	from	variations	310:319	arg1	performance					324:334	performance	324:334	performance	324:334	This study comprehensively investigated the impact of acetate/oleic acid ratios (80%/20%, 60%/40%, 40%/60%, and 20%/80%) on sequencing batch reactor (SBR) with respect to the variations in performance, quorum sensing (QS), and microbial community.
31677408	1	3	from	variations	310:319	arg1	sensing					344:350	quorum sensing	337:350	quorum sensing (QS)	337:355	This study comprehensively investigated the impact of acetate/oleic acid ratios (80%/20%, 60%/40%, 40%/60%, and 20%/80%) on sequencing batch reactor (SBR) with respect to the variations in performance, quorum sensing (QS), and microbial community.
31677408	4	4	theme	sludge	751:756	arg1	properties					758:767	the performance and sludge properties	731:767	properties	758:767	The correlation of QS with the performance and sludge properties was also observed.
31677408	0	5	from	Impact	0:5	arg1	performance					46:56	performance	46:56	performance	46:56	Impact of the acetate/oleic acid ratio on the performance, quorum sensing, and microbial community of sequencing batch reactor system.
31677408	0	5	from	Impact	0:5	arg1	sensing					66:72	quorum sensing	59:72	quorum sensing	59:72	Impact of the acetate/oleic acid ratio on the performance, quorum sensing, and microbial community of sequencing batch reactor system.
31677408	0	5	from	Impact	0:5	arg1	community					89:97	microbial community	79:97	microbial community	79:97	Impact of the acetate/oleic acid ratio on the performance, quorum sensing, and microbial community of sequencing batch reactor system.
31677408	2	6	theme	acetate/oleic	442:454	arg1	ratios					461:466	the acetate/oleic acid ratios	438:466	the acetate/oleic acid ratios	438:466	Results showed that NH+4-N removal was not affected by the acetate/oleic acid ratios, while the COD, total nitrogen (TN), and PO3-4-P removal was considerably affected.
31677408	7	7	theme	ratios	1074:1079	arg1	effect					1045:1050	the effect	1041:1050	the effect of acetate/oleic acid ratios on SBR from microbial viewpoint, which provided insights into fully understanding the essential roles of carbon source on wastewater treatment	1041:1222	This study elucidated the effect of acetate/oleic acid ratios on SBR from microbial viewpoint, which provided insights into fully understanding the essential roles of carbon source on wastewater treatment.
31677408	2	8	theme	total	484:488	arg1	nitrogen					490:497	total nitrogen	484:497	total nitrogen (TN)	484:502	Results showed that NH+4-N removal was not affected by the acetate/oleic acid ratios, while the COD, total nitrogen (TN), and PO3-4-P removal was considerably affected.
31677408	2	8	theme	total	484:488	arg1	TN					500:501	TN	500:501	TN	500:501	Results showed that NH+4-N removal was not affected by the acetate/oleic acid ratios, while the COD, total nitrogen (TN), and PO3-4-P removal was considerably affected.
31677408	1	9	theme	%	227:227	arg1	%					231:231	60%/40%	225:231	60%/40%	225:231	This study comprehensively investigated the impact of acetate/oleic acid ratios (80%/20%, 60%/40%, 40%/60%, and 20%/80%) on sequencing batch reactor (SBR) with respect to the variations in performance, quorum sensing (QS), and microbial community.
31677408	7	10	theme	acid	1069:1072	arg1	ratios					1074:1079	acetate/oleic acid ratios	1055:1079	acetate/oleic acid ratios	1055:1079	This study elucidated the effect of acetate/oleic acid ratios on SBR from microbial viewpoint, which provided insights into fully understanding the essential roles of carbon source on wastewater treatment.
31677408	3	11	theme	increasing	556:565	arg1	acid					573:576	The increasing oleic acid	552:576	The increasing oleic acid ratios	552:583	The increasing oleic acid ratios led to severe sludge bulking, which was significantly positively correlated with proteins/polysaccharides (p < 0.001).
31677408	7	12	theme	source	1193:1198	arg1	roles					1177:1181	the essential roles	1163:1181	the essential roles of carbon source on wastewater treatment	1163:1222	This study elucidated the effect of acetate/oleic acid ratios on SBR from microbial viewpoint, which provided insights into fully understanding the essential roles of carbon source on wastewater treatment.
31677408	5	13	theme	microbial	833:841	arg1	compositions					843:854	microbial compositions	833:854	microbial compositions	833:854	High-throughput sequencing demonstrated that microbial compositions considerably shifted with varying acetate/oleic acid ratios.
31677408	6	14	theme	genera	966:971	arg1	correlation					941:951	the potential correlation	927:951	the potential correlation of bacterial genera with the SBR performance and QS	927:1003	Moreover, the potential correlation of bacterial genera with the SBR performance and QS was proposed.
31677408	0	15	theme	microbial	79:87	arg1	community					89:97	microbial community	79:97	microbial community	79:97	Impact of the acetate/oleic acid ratio on the performance, quorum sensing, and microbial community of sequencing batch reactor system.
31677408	6	16	theme	bacterial	956:964	arg1	genera					966:971	bacterial genera	956:971	bacterial genera	956:971	Moreover, the potential correlation of bacterial genera with the SBR performance and QS was proposed.
31677408	1	17	theme	sequencing	259:268	arg1	SBR					285:287	SBR	285:287	SBR	285:287	This study comprehensively investigated the impact of acetate/oleic acid ratios (80%/20%, 60%/40%, 40%/60%, and 20%/80%) on sequencing batch reactor (SBR) with respect to the variations in performance, quorum sensing (QS), and microbial community.
31677408	1	17	theme	sequencing	259:268	arg1	reactor					276:282	sequencing batch reactor	259:282	sequencing batch reactor (SBR)	259:288	This study comprehensively investigated the impact of acetate/oleic acid ratios (80%/20%, 60%/40%, 40%/60%, and 20%/80%) on sequencing batch reactor (SBR) with respect to the variations in performance, quorum sensing (QS), and microbial community.
31677408	7	18	theme	microbial	1093:1101	arg1	viewpoint					1103:1111	microbial viewpoint	1093:1111	microbial viewpoint	1093:1111	This study elucidated the effect of acetate/oleic acid ratios on SBR from microbial viewpoint, which provided insights into fully understanding the essential roles of carbon source on wastewater treatment.
31677408	1	19	theme	quorum	337:342	arg1	QS					353:354	QS	353:354	QS	353:354	This study comprehensively investigated the impact of acetate/oleic acid ratios (80%/20%, 60%/40%, 40%/60%, and 20%/80%) on sequencing batch reactor (SBR) with respect to the variations in performance, quorum sensing (QS), and microbial community.
31677408	1	19	theme	quorum	337:342	arg1	sensing					344:350	quorum sensing	337:350	quorum sensing (QS)	337:355	This study comprehensively investigated the impact of acetate/oleic acid ratios (80%/20%, 60%/40%, 40%/60%, and 20%/80%) on sequencing batch reactor (SBR) with respect to the variations in performance, quorum sensing (QS), and microbial community.
31677408	7	20	from	effect	1045:1050	arg1	SBR					1084:1086	SBR	1084:1086	SBR	1084:1086	This study elucidated the effect of acetate/oleic acid ratios on SBR from microbial viewpoint, which provided insights into fully understanding the essential roles of carbon source on wastewater treatment.
31677408	7	21	theme	acetate/oleic	1055:1067	arg1	ratios					1074:1079	acetate/oleic acid ratios	1055:1079	acetate/oleic acid ratios	1055:1079	This study elucidated the effect of acetate/oleic acid ratios on SBR from microbial viewpoint, which provided insights into fully understanding the essential roles of carbon source on wastewater treatment.
31677408	7	22	theme	wastewater	1203:1212	arg1	treatment					1214:1222	wastewater treatment	1203:1222	wastewater treatment	1203:1222	This study elucidated the effect of acetate/oleic acid ratios on SBR from microbial viewpoint, which provided insights into fully understanding the essential roles of carbon source on wastewater treatment.
31677408	0	23	theme	acetate/oleic	14:26	arg1	ratio					33:37	the acetate/oleic acid ratio	10:37	the acetate/oleic acid ratio	10:37	Impact of the acetate/oleic acid ratio on the performance, quorum sensing, and microbial community of sequencing batch reactor system.
31677408	0	24	theme	sequencing	102:111	arg1	reactor					119:125	sequencing batch reactor	102:125	sequencing batch reactor system	102:132	Impact of the acetate/oleic acid ratio on the performance, quorum sensing, and microbial community of sequencing batch reactor system.
31677408	5	25	theme	High-throughput	788:802	arg1	sequencing					804:813	High-throughput sequencing	788:813	High-throughput sequencing	788:813	High-throughput sequencing demonstrated that microbial compositions considerably shifted with varying acetate/oleic acid ratios.
31677408	6	26	theme	potential	931:939	arg1	correlation					941:951	the potential correlation	927:951	the potential correlation of bacterial genera with the SBR performance and QS	927:1003	Moreover, the potential correlation of bacterial genera with the SBR performance and QS was proposed.
31677408	3	27	theme	oleic	567:571	arg1	acid					573:576	The increasing oleic acid	552:576	The increasing oleic acid ratios	552:583	The increasing oleic acid ratios led to severe sludge bulking, which was significantly positively correlated with proteins/polysaccharides (p < 0.001).
31677408	2	28	theme	NH+4-N	403:408	arg1	removal					410:416	NH+4-N removal	403:416	NH+4-N removal	403:416	Results showed that NH+4-N removal was not affected by the acetate/oleic acid ratios, while the COD, total nitrogen (TN), and PO3-4-P removal was considerably affected.
31677408	1	29	theme	batch	270:274	arg1	SBR					285:287	SBR	285:287	SBR	285:287	This study comprehensively investigated the impact of acetate/oleic acid ratios (80%/20%, 60%/40%, 40%/60%, and 20%/80%) on sequencing batch reactor (SBR) with respect to the variations in performance, quorum sensing (QS), and microbial community.
31677408	1	29	theme	batch	270:274	arg1	reactor					276:282	sequencing batch reactor	259:282	sequencing batch reactor (SBR)	259:288	This study comprehensively investigated the impact of acetate/oleic acid ratios (80%/20%, 60%/40%, 40%/60%, and 20%/80%) on sequencing batch reactor (SBR) with respect to the variations in performance, quorum sensing (QS), and microbial community.
31677408	0	30	dep	performance	46:56	arg1	the					42:44	the	42:44	the	42:44	Impact of the acetate/oleic acid ratio on the performance, quorum sensing, and microbial community of sequencing batch reactor system.
31677408	1	31	theme	acetate/oleic	189:201	arg1	ratios					208:213	acetate/oleic acid ratios	189:213	acetate/oleic acid ratios (80%/20%, 60%/40%, 40%/60%, and 20%/80%)	189:254	This study comprehensively investigated the impact of acetate/oleic acid ratios (80%/20%, 60%/40%, 40%/60%, and 20%/80%) on sequencing batch reactor (SBR) with respect to the variations in performance, quorum sensing (QS), and microbial community.
31677408	0	32	theme	ratio	33:37	arg1	Impact					0:5	Impact	0:5	Impact of the acetate/oleic acid ratio on the performance, quorum sensing, and microbial community of sequencing batch reactor system.	0:133	Impact of the acetate/oleic acid ratio on the performance, quorum sensing, and microbial community of sequencing batch reactor system.
31677408	3	33	theme	acid	573:576	arg1	ratios					578:583	The increasing oleic acid ratios	552:583	The increasing oleic acid ratios	552:583	The increasing oleic acid ratios led to severe sludge bulking, which was significantly positively correlated with proteins/polysaccharides (p < 0.001).
31677408	0	34	theme	reactor	119:125	arg1	system					127:132	sequencing batch reactor system	102:132	sequencing batch reactor system	102:132	Impact of the acetate/oleic acid ratio on the performance, quorum sensing, and microbial community of sequencing batch reactor system.
31677408	6	35	with	correlation	941:951	arg1	QS					1002:1003	QS	1002:1003	QS	1002:1003	Moreover, the potential correlation of bacterial genera with the SBR performance and QS was proposed.
31677408	6	35	with	correlation	941:951	arg1	performance					986:996	the SBR performance	978:996	the SBR performance	978:996	Moreover, the potential correlation of bacterial genera with the SBR performance and QS was proposed.
31677408	5	36	theme	acetate/oleic	890:902	arg1	ratios					909:914	acetate/oleic acid ratios	890:914	acetate/oleic acid ratios	890:914	High-throughput sequencing demonstrated that microbial compositions considerably shifted with varying acetate/oleic acid ratios.
31677408	1	37	theme	acid	203:206	arg1	ratios					208:213	acetate/oleic acid ratios	189:213	acetate/oleic acid ratios (80%/20%, 60%/40%, 40%/60%, and 20%/80%)	189:254	This study comprehensively investigated the impact of acetate/oleic acid ratios (80%/20%, 60%/40%, 40%/60%, and 20%/80%) on sequencing batch reactor (SBR) with respect to the variations in performance, quorum sensing (QS), and microbial community.
31677408	0	38	theme	acid	28:31	arg1	ratio					33:37	the acetate/oleic acid ratio	10:37	the acetate/oleic acid ratio	10:37	Impact of the acetate/oleic acid ratio on the performance, quorum sensing, and microbial community of sequencing batch reactor system.
31677408	1	39	theme	%	236:236	arg1	%					240:240	40%/60%	234:240	40%/60%	234:240	This study comprehensively investigated the impact of acetate/oleic acid ratios (80%/20%, 60%/40%, 40%/60%, and 20%/80%) on sequencing batch reactor (SBR) with respect to the variations in performance, quorum sensing (QS), and microbial community.
31677408	0	40	theme	batch	113:117	arg1	reactor					119:125	sequencing batch reactor	102:125	sequencing batch reactor system	102:132	Impact of the acetate/oleic acid ratio on the performance, quorum sensing, and microbial community of sequencing batch reactor system.
31677408	5	41	theme	acid	904:907	arg1	ratios					909:914	acetate/oleic acid ratios	890:914	acetate/oleic acid ratios	890:914	High-throughput sequencing demonstrated that microbial compositions considerably shifted with varying acetate/oleic acid ratios.
31677408	7	42	from	roles	1177:1181	arg1	treatment					1214:1222	wastewater treatment	1203:1222	wastewater treatment	1203:1222	This study elucidated the effect of acetate/oleic acid ratios on SBR from microbial viewpoint, which provided insights into fully understanding the essential roles of carbon source on wastewater treatment.
31677408	1	43	theme	ratios	208:213	arg1	impact					179:184	the impact	175:184	the impact of acetate/oleic acid ratios (80%/20%, 60%/40%, 40%/60%, and 20%/80%) on sequencing batch reactor (SBR)	175:288	This study comprehensively investigated the impact of acetate/oleic acid ratios (80%/20%, 60%/40%, 40%/60%, and 20%/80%) on sequencing batch reactor (SBR) with respect to the variations in performance, quorum sensing (QS), and microbial community.
31677408	3	44	theme	sludge	599:604	arg1	bulking					606:612	severe sludge bulking	592:612	severe sludge bulking	592:612	The increasing oleic acid ratios led to severe sludge bulking, which was significantly positively correlated with proteins/polysaccharides (p < 0.001).
31677408	0	45	theme	system	127:132	arg1	performance					46:56	performance	46:56	performance	46:56	Impact of the acetate/oleic acid ratio on the performance, quorum sensing, and microbial community of sequencing batch reactor system.
31677408	0	45	theme	system	127:132	arg1	sensing					66:72	quorum sensing	59:72	quorum sensing	59:72	Impact of the acetate/oleic acid ratio on the performance, quorum sensing, and microbial community of sequencing batch reactor system.
31677408	0	45	theme	system	127:132	arg1	community					89:97	microbial community	79:97	microbial community	79:97	Impact of the acetate/oleic acid ratio on the performance, quorum sensing, and microbial community of sequencing batch reactor system.
31677408	4	46	theme	QS	723:724	arg1	correlation					708:718	The correlation	704:718	The correlation of QS with the performance and sludge properties	704:767	The correlation of QS with the performance and sludge properties was also observed.
31677408	3	47	theme	severe	592:597	arg1	bulking					606:612	severe sludge bulking	592:612	severe sludge bulking	592:612	The increasing oleic acid ratios led to severe sludge bulking, which was significantly positively correlated with proteins/polysaccharides (p < 0.001).
31677408	2	48	theme	PO3-4-P	509:515	arg1	removal					517:523	PO3-4-P removal	509:523	PO3-4-P removal	509:523	Results showed that NH+4-N removal was not affected by the acetate/oleic acid ratios, while the COD, total nitrogen (TN), and PO3-4-P removal was considerably affected.
31677408	4	49	theme	performance	735:745	arg1	properties					758:767	the performance and sludge properties	731:767	properties	758:767	The correlation of QS with the performance and sludge properties was also observed.
31677408	7	50	from	viewpoint	1103:1111	arg1	SBR					1084:1086	SBR	1084:1086	SBR	1084:1086	This study elucidated the effect of acetate/oleic acid ratios on SBR from microbial viewpoint, which provided insights into fully understanding the essential roles of carbon source on wastewater treatment.
31677408	1	51	dep	ratios	208:213	arg1	%					253:253	20%/80%	247:253	20%/80%	247:253	This study comprehensively investigated the impact of acetate/oleic acid ratios (80%/20%, 60%/40%, 40%/60%, and 20%/80%) on sequencing batch reactor (SBR) with respect to the variations in performance, quorum sensing (QS), and microbial community.
31677408	1	51	dep	ratios	208:213	arg1	%					240:240	40%/60%	234:240	40%/60%	234:240	This study comprehensively investigated the impact of acetate/oleic acid ratios (80%/20%, 60%/40%, 40%/60%, and 20%/80%) on sequencing batch reactor (SBR) with respect to the variations in performance, quorum sensing (QS), and microbial community.
31677408	1	51	dep	ratios	208:213	arg1	%					231:231	60%/40%	225:231	60%/40%	225:231	This study comprehensively investigated the impact of acetate/oleic acid ratios (80%/20%, 60%/40%, 40%/60%, and 20%/80%) on sequencing batch reactor (SBR) with respect to the variations in performance, quorum sensing (QS), and microbial community.
31677408	1	51	dep	ratios	208:213	arg1	%					222:222	80%/20%	216:222	80%/20%	216:222	This study comprehensively investigated the impact of acetate/oleic acid ratios (80%/20%, 60%/40%, 40%/60%, and 20%/80%) on sequencing batch reactor (SBR) with respect to the variations in performance, quorum sensing (QS), and microbial community.
31677408	1	52	theme	microbial	362:370	arg1	community					372:380	microbial community	362:380	microbial community	362:380	This study comprehensively investigated the impact of acetate/oleic acid ratios (80%/20%, 60%/40%, 40%/60%, and 20%/80%) on sequencing batch reactor (SBR) with respect to the variations in performance, quorum sensing (QS), and microbial community.
31677408	4	53	with	correlation	708:718	arg1	properties					758:767	the performance and sludge properties	731:767	properties	758:767	The correlation of QS with the performance and sludge properties was also observed.
31677408	1	54	from	impact	179:184	arg1	SBR					285:287	SBR	285:287	SBR	285:287	This study comprehensively investigated the impact of acetate/oleic acid ratios (80%/20%, 60%/40%, 40%/60%, and 20%/80%) on sequencing batch reactor (SBR) with respect to the variations in performance, quorum sensing (QS), and microbial community.
31677408	1	54	from	impact	179:184	arg1	reactor					276:282	sequencing batch reactor	259:282	sequencing batch reactor (SBR)	259:288	This study comprehensively investigated the impact of acetate/oleic acid ratios (80%/20%, 60%/40%, 40%/60%, and 20%/80%) on sequencing batch reactor (SBR) with respect to the variations in performance, quorum sensing (QS), and microbial community.
31677408	7	55	theme	carbon	1186:1191	arg1	source					1193:1198	carbon source	1186:1198	carbon source	1186:1198	This study elucidated the effect of acetate/oleic acid ratios on SBR from microbial viewpoint, which provided insights into fully understanding the essential roles of carbon source on wastewater treatment.
31677408	1	56	theme	%	218:218	arg1	%					222:222	80%/20%	216:222	80%/20%	216:222	This study comprehensively investigated the impact of acetate/oleic acid ratios (80%/20%, 60%/40%, 40%/60%, and 20%/80%) on sequencing batch reactor (SBR) with respect to the variations in performance, quorum sensing (QS), and microbial community.
31677408	7	57	theme	essential	1167:1175	arg1	roles					1177:1181	the essential roles	1163:1181	the essential roles of carbon source on wastewater treatment	1163:1222	This study elucidated the effect of acetate/oleic acid ratios on SBR from microbial viewpoint, which provided insights into fully understanding the essential roles of carbon source on wastewater treatment.
31677408	6	58	theme	SBR	982:984	arg1	performance					986:996	the SBR performance	978:996	the SBR performance	978:996	Moreover, the potential correlation of bacterial genera with the SBR performance and QS was proposed.
30505389	5	0	theme	245-kb	781:786	arg1	island					796:801	a 245-kb genomic island	779:801	a 245-kb genomic island	779:801	Moreover, the genome contained a 245-kb genomic island and a 15-kb incomplete prophage region.
30505389	7	1	theme	genetic	1034:1040	arg1	basis					1042:1046	genetic basis	1034:1046	genetic basis for adapting to the diverse extreme Arctic environments	1034:1102	These related genes and metabolic characteristics revealed genetic basis for adapting to the diverse extreme Arctic environments.
30505389	3	2	theme	GC	541:542	arg1	content					544:550	an average GC content	530:550	an average GC content of 37.20%	530:560	Here, we reported the complete genome sequence of A. luteifluviistationis SM1504T, which comprises 5,379,839 bp with an average GC content of 37.20%.
30505389	5	3	theme	genomic	788:794	arg1	island					796:801	a 245-kb genomic island	779:801	a 245-kb genomic island	779:801	Moreover, the genome contained a 245-kb genomic island and a 15-kb incomplete prophage region.
30505389	6	4	theme	polysaccharides	935:949	arg1	utilization					951:961	polysaccharides utilization	935:961	polysaccharides utilization	935:961	A great percentage of proteins belonging to carbohydrate metabolism especially in regard to polysaccharides utilization were found.
30505389	7	5	theme	metabolic	999:1007	arg1	characteristics					1009:1023	metabolic characteristics	999:1023	metabolic characteristics	999:1023	These related genes and metabolic characteristics revealed genetic basis for adapting to the diverse extreme Arctic environments.
30505389	1	6	theme	Arctic	204:209	arg1	seawater					219:226	Arctic surface seawater	204:226	Arctic surface seawater	204:226	Arcticibacterium luteifluviistationis SM1504T was isolated from Arctic surface seawater and classified as a novel genus of the phylum Bacteroides.
30505389	0	7	theme	Arctic	115:120	arg1	seawater					130:137	Arctic surface seawater	115:137	Arctic surface seawater	115:137	Complete genome sequence of Arcticibacterium luteifluviistationis SM1504T, a cytophagaceae bacterium isolated from Arctic surface seawater.
30505389	5	8	contain	contained	769:777	arg2	island					796:801	a 245-kb genomic island	779:801	a 245-kb genomic island	779:801	Moreover, the genome contained a 245-kb genomic island and a 15-kb incomplete prophage region.
30505389	5	8	contain	contained	769:777	arg1	genome					762:767	the genome	758:767	the genome	758:767	Moreover, the genome contained a 245-kb genomic island and a 15-kb incomplete prophage region.
30505389	5	8	contain	contained	769:777	arg2	region					835:840	a 15-kb incomplete prophage region	807:840	a 15-kb incomplete prophage region	807:840	Moreover, the genome contained a 245-kb genomic island and a 15-kb incomplete prophage region.
30505389	1	9	attach	isolated	190:197	arg2	genus					254:258	a novel genus	246:258	a novel genus of the phylum Bacteroides	246:284	Arcticibacterium luteifluviistationis SM1504T was isolated from Arctic surface seawater and classified as a novel genus of the phylum Bacteroides.
30505389	1	9	attach	isolated	190:197	arg2	SM1504T					178:184	Arcticibacterium luteifluviistationis SM1504T	140:184	Arcticibacterium luteifluviistationis SM1504T	140:184	Arcticibacterium luteifluviistationis SM1504T was isolated from Arctic surface seawater and classified as a novel genus of the phylum Bacteroides.
30505389	1	9	attach	isolated	190:197	arg1	seawater					219:226	Arctic surface seawater	204:226	Arctic surface seawater	204:226	Arcticibacterium luteifluviistationis SM1504T was isolated from Arctic surface seawater and classified as a novel genus of the phylum Bacteroides.
30505389	8	10	theme	carbon	1232:1237	arg1	Arcticibacterium					1188:1203	the genus Arcticibacterium	1178:1203	the genus Arcticibacterium	1178:1203	The genome sequence of A. luteifluviistationis SM1504T also implied that the genus Arcticibacterium may act as a vital organic carbon matter decomposer in the Arctic seawater ecosystem.
30505389	8	10	theme	carbon	1232:1237	arg1	decomposer					1246:1255	a vital organic carbon matter decomposer	1216:1255	a vital organic carbon matter decomposer in the Arctic seawater ecosystem	1216:1288	The genome sequence of A. luteifluviistationis SM1504T also implied that the genus Arcticibacterium may act as a vital organic carbon matter decomposer in the Arctic seawater ecosystem.
30505389	4	11	dep	stress	588:593	arg1	such					596:599	such	596:599	such	596:599	Genes related to various stress (such as radiation, osmosis and antibiotics) resistance and gene clusters coding for carotenoid and flexirubin biosynthesis were detected in the genome.
30505389	8	12	theme	genome	1109:1114	arg1	sequence					1116:1123	The genome sequence	1105:1123	The genome sequence of A. luteifluviistationis SM1504T	1105:1158	The genome sequence of A. luteifluviistationis SM1504T also implied that the genus Arcticibacterium may act as a vital organic carbon matter decomposer in the Arctic seawater ecosystem.
30505389	5	13	theme	incomplete	815:824	arg1	region					835:840	a 15-kb incomplete prophage region	807:840	a 15-kb incomplete prophage region	807:840	Moreover, the genome contained a 245-kb genomic island and a 15-kb incomplete prophage region.
30505389	2	14	theme	Arcticibacterium	299:314	arg1	genomes					316:322	no Arcticibacterium genomes	296:322	no Arcticibacterium genomes	296:322	To date, no Arcticibacterium genomes have been reported, their genomic compositions and metabolic features are still unknown.
30505389	1	15	theme	surface	211:217	arg1	seawater					219:226	Arctic surface seawater	204:226	Arctic surface seawater	204:226	Arcticibacterium luteifluviistationis SM1504T was isolated from Arctic surface seawater and classified as a novel genus of the phylum Bacteroides.
30505389	0	16	theme	surface	122:128	arg1	seawater					130:137	Arctic surface seawater	115:137	Arctic surface seawater	115:137	Complete genome sequence of Arcticibacterium luteifluviistationis SM1504T, a cytophagaceae bacterium isolated from Arctic surface seawater.
30505389	3	17	theme	complete	435:442	arg1	sequence					451:458	the complete genome sequence	431:458	the complete genome sequence of A. luteifluviistationis SM1504T, which comprises 5,379,839 bp with an average GC content of 37.20%	431:560	Here, we reported the complete genome sequence of A. luteifluviistationis SM1504T, which comprises 5,379,839 bp with an average GC content of 37.20%.
30505389	5	18	theme	prophage	826:833	arg1	region					835:840	a 15-kb incomplete prophage region	807:840	a 15-kb incomplete prophage region	807:840	Moreover, the genome contained a 245-kb genomic island and a 15-kb incomplete prophage region.
30505389	0	19	theme	genome	9:14	arg1	sequence					16:23	Complete genome sequence	0:23	Complete genome sequence of Arcticibacterium luteifluviistationis SM1504T	0:72	Complete genome sequence of Arcticibacterium luteifluviistationis SM1504T, a cytophagaceae bacterium isolated from Arctic surface seawater.
30505389	0	19	theme	genome	9:14	arg1	bacterium					91:99	a cytophagaceae bacterium	75:99	a cytophagaceae bacterium isolated from Arctic surface seawater	75:137	Complete genome sequence of Arcticibacterium luteifluviistationis SM1504T, a cytophagaceae bacterium isolated from Arctic surface seawater.
30505389	4	20	located	detected	724:731	arg2	clusters					660:667	gene clusters	655:667	gene clusters	655:667	Genes related to various stress (such as radiation, osmosis and antibiotics) resistance and gene clusters coding for carotenoid and flexirubin biosynthesis were detected in the genome.
30505389	4	20	located	detected	724:731	arg1	genome					740:745	the genome	736:745	the genome	736:745	Genes related to various stress (such as radiation, osmosis and antibiotics) resistance and gene clusters coding for carotenoid and flexirubin biosynthesis were detected in the genome.
30505389	4	20	located	detected	724:731	arg2	Genes					563:567	Genes	563:567	Genes related to various stress (such as radiation, osmosis and antibiotics) resistance	563:649	Genes related to various stress (such as radiation, osmosis and antibiotics) resistance and gene clusters coding for carotenoid and flexirubin biosynthesis were detected in the genome.
30505389	7	21	theme	related	981:987	arg1	genes					989:993	related genes	981:993	related genes	981:993	These related genes and metabolic characteristics revealed genetic basis for adapting to the diverse extreme Arctic environments.
30505389	0	22	theme	Complete	0:7	arg1	sequence					16:23	Complete genome sequence	0:23	Complete genome sequence of Arcticibacterium luteifluviistationis SM1504T	0:72	Complete genome sequence of Arcticibacterium luteifluviistationis SM1504T, a cytophagaceae bacterium isolated from Arctic surface seawater.
30505389	0	22	theme	Complete	0:7	arg1	bacterium					91:99	a cytophagaceae bacterium	75:99	a cytophagaceae bacterium isolated from Arctic surface seawater	75:137	Complete genome sequence of Arcticibacterium luteifluviistationis SM1504T, a cytophagaceae bacterium isolated from Arctic surface seawater.
30505389	4	23	theme	various	580:586	arg1	stress					588:593	various stress	580:593	various stress (such as radiation, osmosis and antibiotics) resistance	580:649	Genes related to various stress (such as radiation, osmosis and antibiotics) resistance and gene clusters coding for carotenoid and flexirubin biosynthesis were detected in the genome.
30505389	4	24	theme	carotenoid	680:689	arg1	biosynthesis					706:717	carotenoid and flexirubin biosynthesis	680:717	biosynthesis	706:717	Genes related to various stress (such as radiation, osmosis and antibiotics) resistance and gene clusters coding for carotenoid and flexirubin biosynthesis were detected in the genome.
30505389	8	25	theme	organic	1224:1230	arg1	Arcticibacterium					1188:1203	the genus Arcticibacterium	1178:1203	the genus Arcticibacterium	1178:1203	The genome sequence of A. luteifluviistationis SM1504T also implied that the genus Arcticibacterium may act as a vital organic carbon matter decomposer in the Arctic seawater ecosystem.
30505389	8	25	theme	organic	1224:1230	arg1	decomposer					1246:1255	a vital organic carbon matter decomposer	1216:1255	a vital organic carbon matter decomposer in the Arctic seawater ecosystem	1216:1288	The genome sequence of A. luteifluviistationis SM1504T also implied that the genus Arcticibacterium may act as a vital organic carbon matter decomposer in the Arctic seawater ecosystem.
30505389	8	26	theme	luteifluviistationis	1131:1150	arg1	SM1504T					1152:1158	A. luteifluviistationis SM1504T	1128:1158	A. luteifluviistationis SM1504T	1128:1158	The genome sequence of A. luteifluviistationis SM1504T also implied that the genus Arcticibacterium may act as a vital organic carbon matter decomposer in the Arctic seawater ecosystem.
30505389	8	27	theme	seawater	1271:1278	arg1	ecosystem					1280:1288	the Arctic seawater ecosystem	1260:1288	the Arctic seawater ecosystem	1260:1288	The genome sequence of A. luteifluviistationis SM1504T also implied that the genus Arcticibacterium may act as a vital organic carbon matter decomposer in the Arctic seawater ecosystem.
30505389	4	28	theme	stress	588:593	arg1	resistance					640:649	various stress (such as radiation, osmosis and antibiotics) resistance	580:649	various stress (such as radiation, osmosis and antibiotics) resistance	580:649	Genes related to various stress (such as radiation, osmosis and antibiotics) resistance and gene clusters coding for carotenoid and flexirubin biosynthesis were detected in the genome.
30505389	3	29	theme	%	560:560	arg1	content					544:550	an average GC content	530:550	an average GC content of 37.20%	530:560	Here, we reported the complete genome sequence of A. luteifluviistationis SM1504T, which comprises 5,379,839 bp with an average GC content of 37.20%.
30505389	6	30	theme	carbohydrate	887:898	arg1	metabolism					900:909	carbohydrate metabolism	887:909	carbohydrate metabolism	887:909	A great percentage of proteins belonging to carbohydrate metabolism especially in regard to polysaccharides utilization were found.
30505389	0	31	theme	luteifluviistationis	45:64	arg1	SM1504T					66:72	Arcticibacterium luteifluviistationis SM1504T	28:72	Arcticibacterium luteifluviistationis SM1504T	28:72	Complete genome sequence of Arcticibacterium luteifluviistationis SM1504T, a cytophagaceae bacterium isolated from Arctic surface seawater.
30505389	3	32	theme	average	533:539	arg1	content					544:550	an average GC content	530:550	an average GC content of 37.20%	530:560	Here, we reported the complete genome sequence of A. luteifluviistationis SM1504T, which comprises 5,379,839 bp with an average GC content of 37.20%.
30505389	3	33	theme	SM1504T	487:493	arg1	sequence					451:458	the complete genome sequence	431:458	the complete genome sequence of A. luteifluviistationis SM1504T, which comprises 5,379,839 bp with an average GC content of 37.20%	431:560	Here, we reported the complete genome sequence of A. luteifluviistationis SM1504T, which comprises 5,379,839 bp with an average GC content of 37.20%.
30505389	8	34	theme	A.	1128:1129	arg1	SM1504T					1152:1158	A. luteifluviistationis SM1504T	1128:1158	A. luteifluviistationis SM1504T	1128:1158	The genome sequence of A. luteifluviistationis SM1504T also implied that the genus Arcticibacterium may act as a vital organic carbon matter decomposer in the Arctic seawater ecosystem.
30505389	1	35	theme	novel	248:252	arg1	SM1504T					178:184	Arcticibacterium luteifluviistationis SM1504T	140:184	Arcticibacterium luteifluviistationis SM1504T	140:184	Arcticibacterium luteifluviistationis SM1504T was isolated from Arctic surface seawater and classified as a novel genus of the phylum Bacteroides.
30505389	1	35	theme	novel	248:252	arg1	genus					254:258	a novel genus	246:258	a novel genus of the phylum Bacteroides	246:284	Arcticibacterium luteifluviistationis SM1504T was isolated from Arctic surface seawater and classified as a novel genus of the phylum Bacteroides.
30505389	3	36	theme	genome	444:449	arg1	sequence					451:458	the complete genome sequence	431:458	the complete genome sequence of A. luteifluviistationis SM1504T, which comprises 5,379,839 bp with an average GC content of 37.20%	431:560	Here, we reported the complete genome sequence of A. luteifluviistationis SM1504T, which comprises 5,379,839 bp with an average GC content of 37.20%.
30505389	0	37	theme	Arcticibacterium	28:43	arg1	SM1504T					66:72	Arcticibacterium luteifluviistationis SM1504T	28:72	Arcticibacterium luteifluviistationis SM1504T	28:72	Complete genome sequence of Arcticibacterium luteifluviistationis SM1504T, a cytophagaceae bacterium isolated from Arctic surface seawater.
30505389	4	38	theme	gene	655:658	arg1	clusters					660:667	gene clusters	655:667	gene clusters	655:667	Genes related to various stress (such as radiation, osmosis and antibiotics) resistance and gene clusters coding for carotenoid and flexirubin biosynthesis were detected in the genome.
30505389	8	39	theme	vital	1218:1222	arg1	Arcticibacterium					1188:1203	the genus Arcticibacterium	1178:1203	the genus Arcticibacterium	1178:1203	The genome sequence of A. luteifluviistationis SM1504T also implied that the genus Arcticibacterium may act as a vital organic carbon matter decomposer in the Arctic seawater ecosystem.
30505389	8	39	theme	vital	1218:1222	arg1	decomposer					1246:1255	a vital organic carbon matter decomposer	1216:1255	a vital organic carbon matter decomposer in the Arctic seawater ecosystem	1216:1288	The genome sequence of A. luteifluviistationis SM1504T also implied that the genus Arcticibacterium may act as a vital organic carbon matter decomposer in the Arctic seawater ecosystem.
30505389	5	40	theme	15-kb	809:813	arg1	region					835:840	a 15-kb incomplete prophage region	807:840	a 15-kb incomplete prophage region	807:840	Moreover, the genome contained a 245-kb genomic island and a 15-kb incomplete prophage region.
30505389	6	41	theme	proteins	865:872	arg1	percentage					851:860	A great percentage	843:860	A great percentage of proteins belonging to carbohydrate metabolism especially in regard to polysaccharides utilization	843:961	A great percentage of proteins belonging to carbohydrate metabolism especially in regard to polysaccharides utilization were found.
30505389	7	42	theme	diverse	1068:1074	arg1	environments					1091:1102	the diverse extreme Arctic environments	1064:1102	the diverse extreme Arctic environments	1064:1102	These related genes and metabolic characteristics revealed genetic basis for adapting to the diverse extreme Arctic environments.
30505389	2	43	theme	metabolic	375:383	arg1	features					385:392	metabolic features	375:392	metabolic features	375:392	To date, no Arcticibacterium genomes have been reported, their genomic compositions and metabolic features are still unknown.
30505389	0	44	theme	SM1504T	66:72	arg1	sequence					16:23	Complete genome sequence	0:23	Complete genome sequence of Arcticibacterium luteifluviistationis SM1504T	0:72	Complete genome sequence of Arcticibacterium luteifluviistationis SM1504T, a cytophagaceae bacterium isolated from Arctic surface seawater.
30505389	0	44	theme	SM1504T	66:72	arg1	bacterium					91:99	a cytophagaceae bacterium	75:99	a cytophagaceae bacterium isolated from Arctic surface seawater	75:137	Complete genome sequence of Arcticibacterium luteifluviistationis SM1504T, a cytophagaceae bacterium isolated from Arctic surface seawater.
30505389	4	45	theme	related	569:575	arg1	Genes					563:567	Genes	563:567	Genes related to various stress (such as radiation, osmosis and antibiotics) resistance	563:649	Genes related to various stress (such as radiation, osmosis and antibiotics) resistance and gene clusters coding for carotenoid and flexirubin biosynthesis were detected in the genome.
30505389	8	46	from	decomposer	1246:1255	arg1	ecosystem					1280:1288	the Arctic seawater ecosystem	1260:1288	the Arctic seawater ecosystem	1260:1288	The genome sequence of A. luteifluviistationis SM1504T also implied that the genus Arcticibacterium may act as a vital organic carbon matter decomposer in the Arctic seawater ecosystem.
30505389	1	47	theme	Arcticibacterium	140:155	arg1	SM1504T					178:184	Arcticibacterium luteifluviistationis SM1504T	140:184	Arcticibacterium luteifluviistationis SM1504T	140:184	Arcticibacterium luteifluviistationis SM1504T was isolated from Arctic surface seawater and classified as a novel genus of the phylum Bacteroides.
30505389	1	47	theme	Arcticibacterium	140:155	arg1	genus					254:258	a novel genus	246:258	a novel genus of the phylum Bacteroides	246:284	Arcticibacterium luteifluviistationis SM1504T was isolated from Arctic surface seawater and classified as a novel genus of the phylum Bacteroides.
30505389	7	48	theme	extreme	1076:1082	arg1	environments					1091:1102	the diverse extreme Arctic environments	1064:1102	the diverse extreme Arctic environments	1064:1102	These related genes and metabolic characteristics revealed genetic basis for adapting to the diverse extreme Arctic environments.
30505389	0	49	attach	isolated	101:108	arg1	seawater					130:137	Arctic surface seawater	115:137	Arctic surface seawater	115:137	Complete genome sequence of Arcticibacterium luteifluviistationis SM1504T, a cytophagaceae bacterium isolated from Arctic surface seawater.
30505389	0	49	attach	isolated	101:108	arg2	sequence					16:23	Complete genome sequence	0:23	Complete genome sequence of Arcticibacterium luteifluviistationis SM1504T	0:72	Complete genome sequence of Arcticibacterium luteifluviistationis SM1504T, a cytophagaceae bacterium isolated from Arctic surface seawater.
30505389	0	49	attach	isolated	101:108	arg2	bacterium					91:99	a cytophagaceae bacterium	75:99	a cytophagaceae bacterium isolated from Arctic surface seawater	75:137	Complete genome sequence of Arcticibacterium luteifluviistationis SM1504T, a cytophagaceae bacterium isolated from Arctic surface seawater.
30505389	1	50	theme	luteifluviistationis	157:176	arg1	SM1504T					178:184	Arcticibacterium luteifluviistationis SM1504T	140:184	Arcticibacterium luteifluviistationis SM1504T	140:184	Arcticibacterium luteifluviistationis SM1504T was isolated from Arctic surface seawater and classified as a novel genus of the phylum Bacteroides.
30505389	1	50	theme	luteifluviistationis	157:176	arg1	genus					254:258	a novel genus	246:258	a novel genus of the phylum Bacteroides	246:284	Arcticibacterium luteifluviistationis SM1504T was isolated from Arctic surface seawater and classified as a novel genus of the phylum Bacteroides.
30505389	7	51	theme	Arctic	1084:1089	arg1	environments					1091:1102	the diverse extreme Arctic environments	1064:1102	the diverse extreme Arctic environments	1064:1102	These related genes and metabolic characteristics revealed genetic basis for adapting to the diverse extreme Arctic environments.
30505389	6	52	theme	great	845:849	arg1	percentage					851:860	A great percentage	843:860	A great percentage of proteins belonging to carbohydrate metabolism especially in regard to polysaccharides utilization	843:961	A great percentage of proteins belonging to carbohydrate metabolism especially in regard to polysaccharides utilization were found.
30505389	8	53	theme	matter	1239:1244	arg1	Arcticibacterium					1188:1203	the genus Arcticibacterium	1178:1203	the genus Arcticibacterium	1178:1203	The genome sequence of A. luteifluviistationis SM1504T also implied that the genus Arcticibacterium may act as a vital organic carbon matter decomposer in the Arctic seawater ecosystem.
30505389	8	53	theme	matter	1239:1244	arg1	decomposer					1246:1255	a vital organic carbon matter decomposer	1216:1255	a vital organic carbon matter decomposer in the Arctic seawater ecosystem	1216:1288	The genome sequence of A. luteifluviistationis SM1504T also implied that the genus Arcticibacterium may act as a vital organic carbon matter decomposer in the Arctic seawater ecosystem.
30505389	8	54	theme	genus	1182:1186	arg1	Arcticibacterium					1188:1203	the genus Arcticibacterium	1178:1203	the genus Arcticibacterium	1178:1203	The genome sequence of A. luteifluviistationis SM1504T also implied that the genus Arcticibacterium may act as a vital organic carbon matter decomposer in the Arctic seawater ecosystem.
30505389	8	54	theme	genus	1182:1186	arg1	decomposer					1246:1255	a vital organic carbon matter decomposer	1216:1255	a vital organic carbon matter decomposer in the Arctic seawater ecosystem	1216:1288	The genome sequence of A. luteifluviistationis SM1504T also implied that the genus Arcticibacterium may act as a vital organic carbon matter decomposer in the Arctic seawater ecosystem.
30505389	2	55	theme	genomic	350:356	arg1	compositions					358:369	their genomic compositions	344:369	their genomic compositions	344:369	To date, no Arcticibacterium genomes have been reported, their genomic compositions and metabolic features are still unknown.
30505389	8	56	theme	Arctic	1264:1269	arg1	ecosystem					1280:1288	the Arctic seawater ecosystem	1260:1288	the Arctic seawater ecosystem	1260:1288	The genome sequence of A. luteifluviistationis SM1504T also implied that the genus Arcticibacterium may act as a vital organic carbon matter decomposer in the Arctic seawater ecosystem.
30505389	0	57	theme	cytophagaceae	77:89	arg1	bacterium					91:99	a cytophagaceae bacterium	75:99	a cytophagaceae bacterium isolated from Arctic surface seawater	75:137	Complete genome sequence of Arcticibacterium luteifluviistationis SM1504T, a cytophagaceae bacterium isolated from Arctic surface seawater.
30505389	0	57	theme	cytophagaceae	77:89	arg1	sequence					16:23	Complete genome sequence	0:23	Complete genome sequence of Arcticibacterium luteifluviistationis SM1504T	0:72	Complete genome sequence of Arcticibacterium luteifluviistationis SM1504T, a cytophagaceae bacterium isolated from Arctic surface seawater.
30505389	1	58	theme	phylum	267:272	arg1	Bacteroides					274:284	the phylum Bacteroides	263:284	the phylum Bacteroides	263:284	Arcticibacterium luteifluviistationis SM1504T was isolated from Arctic surface seawater and classified as a novel genus of the phylum Bacteroides.
30505389	4	59	theme	flexirubin	695:704	arg1	biosynthesis					706:717	carotenoid and flexirubin biosynthesis	680:717	biosynthesis	706:717	Genes related to various stress (such as radiation, osmosis and antibiotics) resistance and gene clusters coding for carotenoid and flexirubin biosynthesis were detected in the genome.
30505389	8	60	theme	SM1504T	1152:1158	arg1	sequence					1116:1123	The genome sequence	1105:1123	The genome sequence of A. luteifluviistationis SM1504T	1105:1158	The genome sequence of A. luteifluviistationis SM1504T also implied that the genus Arcticibacterium may act as a vital organic carbon matter decomposer in the Arctic seawater ecosystem.
30505389	1	61	theme	Bacteroides	274:284	arg1	SM1504T					178:184	Arcticibacterium luteifluviistationis SM1504T	140:184	Arcticibacterium luteifluviistationis SM1504T	140:184	Arcticibacterium luteifluviistationis SM1504T was isolated from Arctic surface seawater and classified as a novel genus of the phylum Bacteroides.
30505389	1	61	theme	Bacteroides	274:284	arg1	genus					254:258	a novel genus	246:258	a novel genus of the phylum Bacteroides	246:284	Arcticibacterium luteifluviistationis SM1504T was isolated from Arctic surface seawater and classified as a novel genus of the phylum Bacteroides.
31109707	10	0	theme	plaque	1477:1482	arg1	calcification					1484:1496	plaque calcification	1477:1496	plaque calcification	1477:1496	Proteoglycan 4 (PRG4) was identified as the most upregulated gene in association with plaque calcification and found in the ECM, SMA+ and CD68+/TRAP + cells.
31109707	8	1	theme	enriched	1130:1137	arg1	processes					1139:1147	The most enriched processes	1121:1147	The most enriched processes in high-calcified plaques	1121:1173	The most enriched processes in high-calcified plaques were related to SMCs and extracellular matrix (ECM) organization, while inflammation, lipid transport and chemokine signaling were repressed.
31109707	8	1	theme	enriched	1130:1137	arg1	related					1180:1186	related	1180:1186	related	1180:1186	The most enriched processes in high-calcified plaques were related to SMCs and extracellular matrix (ECM) organization, while inflammation, lipid transport and chemokine signaling were repressed.
31109707	9	2	theme	medial	1369:1374	arg1	calcification					1376:1388	high medial calcification	1364:1388	high medial calcification	1364:1388	These findings were confirmed in arteries with high medial calcification.
31109707	11	3	theme	stable	1654:1659	arg1	plaques					1661:1667	stable plaques	1654:1667	stable plaques	1654:1667	CONCLUSIONS Macro-calcification in carotid lesions correlated with a transcriptional profile typical for stable plaques, with altered SMC phenotype and ECM composition and repressed inflammation.
31109707	7	4	dep	upregulated	987:997	arg1	whereas					1074:1080	whereas	1074:1080	whereas	1074:1080	RESULTS Smooth muscle cell (SMC) markers were upregulated in high-calcified plaques and calcified plaques from symptomatic patients, whereas macrophage markers were downregulated.
31109707	13	5	theme	stroke	2009:2014	arg1	risk					2016:2019	stroke risk	2009:2019	stroke risk	2009:2019	This study strengthens the notion that assessment of calcification may aid evaluation of plaque phenotype and stroke risk.
31109707	7	6	theme	symptomatic	1052:1062	arg1	patients					1064:1071	symptomatic patients	1052:1071	symptomatic patients	1052:1071	RESULTS Smooth muscle cell (SMC) markers were upregulated in high-calcified plaques and calcified plaques from symptomatic patients, whereas macrophage markers were downregulated.
31109707	1	7	dep	BACKGROUND	122:131	arg1	causes					175:180	causes	175:180	causes stroke	175:187	BACKGROUND AND AIMS Unstable carotid atherosclerosis causes stroke, but methods to identify patients and lesions at risk are lacking.
31109707	7	8	theme	cell	963:966	arg1	markers					974:980	Smooth muscle cell (SMC) markers	949:980	Smooth muscle cell (SMC) markers	949:980	RESULTS Smooth muscle cell (SMC) markers were upregulated in high-calcified plaques and calcified plaques from symptomatic patients, whereas macrophage markers were downregulated.
31109707	8	9	theme	lipid	1261:1265	arg1	transport					1267:1275	lipid transport	1261:1275	lipid transport	1261:1275	The most enriched processes in high-calcified plaques were related to SMCs and extracellular matrix (ECM) organization, while inflammation, lipid transport and chemokine signaling were repressed.
31109707	11	10	theme	ECM	1701:1703	arg1	composition					1705:1715	ECM composition	1701:1715	ECM composition	1701:1715	CONCLUSIONS Macro-calcification in carotid lesions correlated with a transcriptional profile typical for stable plaques, with altered SMC phenotype and ECM composition and repressed inflammation.
31109707	2	11	from	patients	361:368	arg1	calcification					310:322	calcification	310:322	calcification in carotid plaques from asymptomatic patients	310:368	We recently found enrichment of genes associated with calcification in carotid plaques from asymptomatic patients.
31109707	2	11	from	patients	361:368	arg1	plaques					335:341	carotid plaques	327:341	carotid plaques from asymptomatic patients	327:368	We recently found enrichment of genes associated with calcification in carotid plaques from asymptomatic patients.
31109707	11	12	theme	typical	1642:1648	arg1	profile					1634:1640	a transcriptional profile	1616:1640	a transcriptional profile typical for stable plaques	1616:1667	CONCLUSIONS Macro-calcification in carotid lesions correlated with a transcriptional profile typical for stable plaques, with altered SMC phenotype and ECM composition and repressed inflammation.
31109707	4	13	theme	CT	652:653	arg1	angiographies					655:667	pre-operative CT angiographies	638:667	pre-operative CT angiographies	638:667	METHODS Plaque calcification was measured in pre-operative CT angiographies.
31109707	3	14	dep	correlates	540:549	arg1	estimated					501:509	estimated	501:509	estimated by computed tomography (CT)	501:537	Here, we hypothesized that calcification represents a stabilising feature of plaques and investigated how macro-calcification, as estimated by computed tomography (CT), correlates with gene expression profiles in lesions.
31109707	10	15	theme	upregulated	1440:1450	arg1	PRG4					1407:1410	Proteoglycan 4 (PRG4)	1391:1411	Proteoglycan 4 (PRG4)	1391:1411	Proteoglycan 4 (PRG4) was identified as the most upregulated gene in association with plaque calcification and found in the ECM, SMA+ and CD68+/TRAP + cells.
31109707	10	15	theme	upregulated	1440:1450	arg1	gene					1452:1455	the most upregulated gene	1431:1455	the most upregulated gene in association with plaque calcification	1431:1496	Proteoglycan 4 (PRG4) was identified as the most upregulated gene in association with plaque calcification and found in the ECM, SMA+ and CD68+/TRAP + cells.
31109707	6	16	theme	medial	882:887	arg1	calcification					889:901	medial calcification	882:901	medial calcification	882:901	Immunohistochemistry and qPCR were performed to evaluate the findings in plaques and arteries with medial calcification from chronic kidney disease patients.
31109707	7	17	theme	Smooth	949:954	arg1	SMC					969:971	SMC	969:971	SMC	969:971	RESULTS Smooth muscle cell (SMC) markers were upregulated in high-calcified plaques and calcified plaques from symptomatic patients, whereas macrophage markers were downregulated.
31109707	7	17	theme	Smooth	949:954	arg1	cell					963:966	Smooth muscle cell	949:966	Smooth muscle cell (SMC) markers	949:980	RESULTS Smooth muscle cell (SMC) markers were upregulated in high-calcified plaques and calcified plaques from symptomatic patients, whereas macrophage markers were downregulated.
31109707	4	18	theme	pre-operative	638:650	arg1	angiographies					655:667	pre-operative CT angiographies	638:667	pre-operative CT angiographies	638:667	METHODS Plaque calcification was measured in pre-operative CT angiographies.
31109707	7	19	theme	muscle	956:961	arg1	SMC					969:971	SMC	969:971	SMC	969:971	RESULTS Smooth muscle cell (SMC) markers were upregulated in high-calcified plaques and calcified plaques from symptomatic patients, whereas macrophage markers were downregulated.
31109707	7	19	theme	muscle	956:961	arg1	cell					963:966	Smooth muscle cell	949:966	Smooth muscle cell (SMC) markers	949:980	RESULTS Smooth muscle cell (SMC) markers were upregulated in high-calcified plaques and calcified plaques from symptomatic patients, whereas macrophage markers were downregulated.
31109707	0	20	theme	plaque	48:53	arg1	transcriptomes					55:68	carotid plaque transcriptomes	40:68	carotid plaque transcriptomes	40:68	Correlation of computed tomography with carotid plaque transcriptomes associates calcification with lesion-stabilization.
31109707	6	21	with	plaques	856:862	arg1	calcification					889:901	medial calcification	882:901	medial calcification	882:901	Immunohistochemistry and qPCR were performed to evaluate the findings in plaques and arteries with medial calcification from chronic kidney disease patients.
31109707	12	22	theme	smooth	1873:1878	arg1	cells					1892:1896	smooth muscle-like cells	1873:1896	smooth muscle-like cells	1873:1896	PRG4, previously not described in atherosclerosis, was enriched in the calcified ECM and localized to activated macrophages and smooth muscle-like cells.
31109707	13	23	theme	risk	2016:2019	arg1	evaluation					1974:1983	evaluation	1974:1983	evaluation of plaque phenotype and stroke risk	1974:2019	This study strengthens the notion that assessment of calcification may aid evaluation of plaque phenotype and stroke risk.
31109707	3	24	theme	stabilising	425:435	arg1	feature					437:443	a stabilising feature	423:443	a stabilising feature of plaques	423:454	Here, we hypothesized that calcification represents a stabilising feature of plaques and investigated how macro-calcification, as estimated by computed tomography (CT), correlates with gene expression profiles in lesions.
31109707	7	25	theme	calcified	1029:1037	arg1	plaques					1039:1045	calcified plaques	1029:1045	calcified plaques	1029:1045	RESULTS Smooth muscle cell (SMC) markers were upregulated in high-calcified plaques and calcified plaques from symptomatic patients, whereas macrophage markers were downregulated.
31109707	2	26	theme	asymptomatic	348:359	arg1	patients					361:368	asymptomatic patients	348:368	asymptomatic patients	348:368	We recently found enrichment of genes associated with calcification in carotid plaques from asymptomatic patients.
31109707	10	27	theme	Proteoglycan	1391:1402	arg1	PRG4					1407:1410	Proteoglycan 4 (PRG4)	1391:1411	Proteoglycan 4 (PRG4)	1391:1411	Proteoglycan 4 (PRG4) was identified as the most upregulated gene in association with plaque calcification and found in the ECM, SMA+ and CD68+/TRAP + cells.
31109707	10	27	theme	Proteoglycan	1391:1402	arg1	gene					1452:1455	the most upregulated gene	1431:1455	the most upregulated gene in association with plaque calcification	1431:1496	Proteoglycan 4 (PRG4) was identified as the most upregulated gene in association with plaque calcification and found in the ECM, SMA+ and CD68+/TRAP + cells.
31109707	10	28	from	gene	1452:1455	arg1	association					1460:1470	association	1460:1470	association with plaque calcification	1460:1496	Proteoglycan 4 (PRG4) was identified as the most upregulated gene in association with plaque calcification and found in the ECM, SMA+ and CD68+/TRAP + cells.
31109707	8	29	theme	chemokine	1281:1289	arg1	signaling					1291:1299	chemokine signaling	1281:1299	chemokine signaling	1281:1299	The most enriched processes in high-calcified plaques were related to SMCs and extracellular matrix (ECM) organization, while inflammation, lipid transport and chemokine signaling were repressed.
31109707	4	30	theme	Plaque	601:606	arg1	calcification					608:620	METHODS Plaque calcification	593:620	METHODS Plaque calcification	593:620	METHODS Plaque calcification was measured in pre-operative CT angiographies.
31109707	7	31	theme	high-calcified	1002:1015	arg1	plaques					1017:1023	high-calcified plaques	1002:1023	high-calcified plaques	1002:1023	RESULTS Smooth muscle cell (SMC) markers were upregulated in high-calcified plaques and calcified plaques from symptomatic patients, whereas macrophage markers were downregulated.
31109707	1	32	dep	methods	194:200	arg1	identify					205:212	identify	205:212	to identify patients and lesions at risk	202:241	BACKGROUND AND AIMS Unstable carotid atherosclerosis causes stroke, but methods to identify patients and lesions at risk are lacking.
31109707	9	33	with	arteries	1350:1357	arg1	calcification					1376:1388	high medial calcification	1364:1388	high medial calcification	1364:1388	These findings were confirmed in arteries with high medial calcification.
31109707	0	34	theme	tomography	24:33	arg1	Correlation					0:10	Correlation	0:10	Correlation of computed tomography with carotid plaque transcriptomes	0:68	Correlation of computed tomography with carotid plaque transcriptomes associates calcification with lesion-stabilization.
31109707	4	35	theme	METHODS	593:599	arg1	calcification					608:620	METHODS Plaque calcification	593:620	METHODS Plaque calcification	593:620	METHODS Plaque calcification was measured in pre-operative CT angiographies.
31109707	5	36	theme	bioinformatic	759:771	arg1	analyses					773:780	bioinformatic analyses	759:780	bioinformatic analyses	759:780	Plaques were sorted into high- and low-calcified, profiled with microarrays, followed by bioinformatic analyses.
31109707	12	37	theme	muscle-like	1880:1890	arg1	cells					1892:1896	smooth muscle-like cells	1873:1896	smooth muscle-like cells	1873:1896	PRG4, previously not described in atherosclerosis, was enriched in the calcified ECM and localized to activated macrophages and smooth muscle-like cells.
31109707	3	38	theme	computed	514:521	arg1	CT					535:536	CT	535:536	CT	535:536	Here, we hypothesized that calcification represents a stabilising feature of plaques and investigated how macro-calcification, as estimated by computed tomography (CT), correlates with gene expression profiles in lesions.
31109707	3	38	theme	computed	514:521	arg1	tomography					523:532	computed tomography	514:532	computed tomography (CT)	514:537	Here, we hypothesized that calcification represents a stabilising feature of plaques and investigated how macro-calcification, as estimated by computed tomography (CT), correlates with gene expression profiles in lesions.
31109707	0	39	theme	computed	15:22	arg1	tomography					24:33	computed tomography	15:33	computed tomography	15:33	Correlation of computed tomography with carotid plaque transcriptomes associates calcification with lesion-stabilization.
31109707	13	40	theme	calcification	1952:1964	arg1	assessment					1938:1947	assessment	1938:1947	assessment of calcification	1938:1964	This study strengthens the notion that assessment of calcification may aid evaluation of plaque phenotype and stroke risk.
31109707	2	41	theme	carotid	327:333	arg1	plaques					335:341	carotid plaques	327:341	carotid plaques from asymptomatic patients	327:368	We recently found enrichment of genes associated with calcification in carotid plaques from asymptomatic patients.
31109707	13	42	theme	plaque	1988:1993	arg1	phenotype					1995:2003	plaque phenotype	1988:2003	plaque phenotype	1988:2003	This study strengthens the notion that assessment of calcification may aid evaluation of plaque phenotype and stroke risk.
31109707	11	43	theme	carotid	1584:1590	arg1	lesions					1592:1598	carotid lesions	1584:1598	carotid lesions	1584:1598	CONCLUSIONS Macro-calcification in carotid lesions correlated with a transcriptional profile typical for stable plaques, with altered SMC phenotype and ECM composition and repressed inflammation.
31109707	0	44	theme	carotid	40:46	arg1	transcriptomes					55:68	carotid plaque transcriptomes	40:68	carotid plaque transcriptomes	40:68	Correlation of computed tomography with carotid plaque transcriptomes associates calcification with lesion-stabilization.
31109707	11	45	theme	CONCLUSIONS	1549:1559	arg1	Macro-calcification					1561:1579	CONCLUSIONS Macro-calcification	1549:1579	CONCLUSIONS Macro-calcification in carotid lesions	1549:1598	CONCLUSIONS Macro-calcification in carotid lesions correlated with a transcriptional profile typical for stable plaques, with altered SMC phenotype and ECM composition and repressed inflammation.
31109707	8	46	theme	extracellular	1200:1212	arg1	ECM					1222:1224	ECM	1222:1224	ECM	1222:1224	The most enriched processes in high-calcified plaques were related to SMCs and extracellular matrix (ECM) organization, while inflammation, lipid transport and chemokine signaling were repressed.
31109707	8	46	theme	extracellular	1200:1212	arg1	matrix					1214:1219	extracellular matrix	1200:1219	extracellular matrix (ECM) organization	1200:1238	The most enriched processes in high-calcified plaques were related to SMCs and extracellular matrix (ECM) organization, while inflammation, lipid transport and chemokine signaling were repressed.
31109707	11	47	from	Macro-calcification	1561:1579	arg1	lesions					1592:1598	carotid lesions	1584:1598	carotid lesions	1584:1598	CONCLUSIONS Macro-calcification in carotid lesions correlated with a transcriptional profile typical for stable plaques, with altered SMC phenotype and ECM composition and repressed inflammation.
31109707	12	48	theme	activated	1847:1855	arg1	macrophages					1857:1867	activated macrophages	1847:1867	activated macrophages	1847:1867	PRG4, previously not described in atherosclerosis, was enriched in the calcified ECM and localized to activated macrophages and smooth muscle-like cells.
31109707	7	49	from	patients	1064:1071	arg1	plaques					1017:1023	high-calcified plaques	1002:1023	high-calcified plaques	1002:1023	RESULTS Smooth muscle cell (SMC) markers were upregulated in high-calcified plaques and calcified plaques from symptomatic patients, whereas macrophage markers were downregulated.
31109707	7	49	from	patients	1064:1071	arg1	plaques					1039:1045	calcified plaques	1029:1045	calcified plaques	1029:1045	RESULTS Smooth muscle cell (SMC) markers were upregulated in high-calcified plaques and calcified plaques from symptomatic patients, whereas macrophage markers were downregulated.
31109707	13	50	theme	phenotype	1995:2003	arg1	evaluation					1974:1983	evaluation	1974:1983	evaluation of plaque phenotype and stroke risk	1974:2019	This study strengthens the notion that assessment of calcification may aid evaluation of plaque phenotype and stroke risk.
31109707	11	51	theme	SMC	1683:1685	arg1	phenotype					1687:1695	altered SMC phenotype	1675:1695	altered SMC phenotype	1675:1695	CONCLUSIONS Macro-calcification in carotid lesions correlated with a transcriptional profile typical for stable plaques, with altered SMC phenotype and ECM composition and repressed inflammation.
31109707	5	52	dep	sorted	683:688	arg1	followed					747:754	followed	747:754	followed by bioinformatic analyses	747:780	Plaques were sorted into high- and low-calcified, profiled with microarrays, followed by bioinformatic analyses.
31109707	8	53	from	processes	1139:1147	arg1	plaques					1167:1173	high-calcified plaques	1152:1173	high-calcified plaques	1152:1173	The most enriched processes in high-calcified plaques were related to SMCs and extracellular matrix (ECM) organization, while inflammation, lipid transport and chemokine signaling were repressed.
31109707	6	54	with	arteries	868:875	arg1	calcification					889:901	medial calcification	882:901	medial calcification	882:901	Immunohistochemistry and qPCR were performed to evaluate the findings in plaques and arteries with medial calcification from chronic kidney disease patients.
31109707	7	55	dep	RESULTS	941:947	arg1	upregulated					987:997	upregulated	987:997	were upregulated in high-calcified plaques and calcified plaques from symptomatic patients	982:1071	RESULTS Smooth muscle cell (SMC) markers were upregulated in high-calcified plaques and calcified plaques from symptomatic patients, whereas macrophage markers were downregulated.
31109707	1	56	theme	Unstable	142:149	arg1	atherosclerosis					159:173	Unstable carotid atherosclerosis	142:173	Unstable carotid atherosclerosis	142:173	BACKGROUND AND AIMS Unstable carotid atherosclerosis causes stroke, but methods to identify patients and lesions at risk are lacking.
31109707	3	57	theme	plaques	448:454	arg1	feature					437:443	a stabilising feature	423:443	a stabilising feature of plaques	423:454	Here, we hypothesized that calcification represents a stabilising feature of plaques and investigated how macro-calcification, as estimated by computed tomography (CT), correlates with gene expression profiles in lesions.
31109707	8	58	theme	matrix	1214:1219	arg1	organization					1227:1238	extracellular matrix (ECM) organization	1200:1238	extracellular matrix (ECM) organization	1200:1238	The most enriched processes in high-calcified plaques were related to SMCs and extracellular matrix (ECM) organization, while inflammation, lipid transport and chemokine signaling were repressed.
31109707	9	59	theme	high	1364:1367	arg1	calcification					1376:1388	high medial calcification	1364:1388	high medial calcification	1364:1388	These findings were confirmed in arteries with high medial calcification.
31109707	8	60	theme	high-calcified	1152:1165	arg1	plaques					1167:1173	high-calcified plaques	1152:1173	high-calcified plaques	1152:1173	The most enriched processes in high-calcified plaques were related to SMCs and extracellular matrix (ECM) organization, while inflammation, lipid transport and chemokine signaling were repressed.
31109707	1	61	theme	carotid	151:157	arg1	atherosclerosis					159:173	Unstable carotid atherosclerosis	142:173	Unstable carotid atherosclerosis	142:173	BACKGROUND AND AIMS Unstable carotid atherosclerosis causes stroke, but methods to identify patients and lesions at risk are lacking.
31109707	11	62	theme	altered	1675:1681	arg1	phenotype					1687:1695	altered SMC phenotype	1675:1695	altered SMC phenotype	1675:1695	CONCLUSIONS Macro-calcification in carotid lesions correlated with a transcriptional profile typical for stable plaques, with altered SMC phenotype and ECM composition and repressed inflammation.
31109707	6	63	theme	disease	923:929	arg1	patients					931:938	chronic kidney disease patients	908:938	chronic kidney disease patients	908:938	Immunohistochemistry and qPCR were performed to evaluate the findings in plaques and arteries with medial calcification from chronic kidney disease patients.
31109707	2	64	theme	genes	288:292	arg1	enrichment					274:283	enrichment	274:283	enrichment of genes associated with calcification in carotid plaques from asymptomatic patients	274:368	We recently found enrichment of genes associated with calcification in carotid plaques from asymptomatic patients.
31109707	0	65	with	Correlation	0:10	arg1	transcriptomes					55:68	carotid plaque transcriptomes	40:68	carotid plaque transcriptomes	40:68	Correlation of computed tomography with carotid plaque transcriptomes associates calcification with lesion-stabilization.
31109707	6	66	from	findings	844:851	arg1	patients					931:938	chronic kidney disease patients	908:938	chronic kidney disease patients	908:938	Immunohistochemistry and qPCR were performed to evaluate the findings in plaques and arteries with medial calcification from chronic kidney disease patients.
31109707	6	66	from	findings	844:851	arg1	plaques					856:862	plaques	856:862	plaques	856:862	Immunohistochemistry and qPCR were performed to evaluate the findings in plaques and arteries with medial calcification from chronic kidney disease patients.
31109707	6	66	from	findings	844:851	arg1	arteries					868:875	arteries	868:875	arteries with medial calcification from chronic kidney disease patients	868:938	Immunohistochemistry and qPCR were performed to evaluate the findings in plaques and arteries with medial calcification from chronic kidney disease patients.
31109707	11	67	theme	transcriptional	1618:1632	arg1	profile					1634:1640	a transcriptional profile	1616:1640	a transcriptional profile typical for stable plaques	1616:1667	CONCLUSIONS Macro-calcification in carotid lesions correlated with a transcriptional profile typical for stable plaques, with altered SMC phenotype and ECM composition and repressed inflammation.
31109707	6	68	theme	kidney	916:921	arg1	patients					931:938	chronic kidney disease patients	908:938	chronic kidney disease patients	908:938	Immunohistochemistry and qPCR were performed to evaluate the findings in plaques and arteries with medial calcification from chronic kidney disease patients.
31109707	7	69	theme	macrophage	1082:1091	arg1	markers					1093:1099	macrophage markers	1082:1099	macrophage markers	1082:1099	RESULTS Smooth muscle cell (SMC) markers were upregulated in high-calcified plaques and calcified plaques from symptomatic patients, whereas macrophage markers were downregulated.
31109707	6	70	theme	chronic	908:914	arg1	patients					931:938	chronic kidney disease patients	908:938	chronic kidney disease patients	908:938	Immunohistochemistry and qPCR were performed to evaluate the findings in plaques and arteries with medial calcification from chronic kidney disease patients.
31109707	12	71	theme	calcified	1816:1824	arg1	ECM					1826:1828	the calcified ECM	1812:1828	the calcified ECM	1812:1828	PRG4, previously not described in atherosclerosis, was enriched in the calcified ECM and localized to activated macrophages and smooth muscle-like cells.
31109707	3	72	theme	gene	556:559	arg1	profiles					572:579	gene expression profiles	556:579	gene expression profiles in lesions	556:590	Here, we hypothesized that calcification represents a stabilising feature of plaques and investigated how macro-calcification, as estimated by computed tomography (CT), correlates with gene expression profiles in lesions.
31109707	2	73	from	calcification	310:322	arg1	plaques					335:341	carotid plaques	327:341	carotid plaques from asymptomatic patients	327:368	We recently found enrichment of genes associated with calcification in carotid plaques from asymptomatic patients.
31109707	2	73	from	calcification	310:322	arg1	patients					361:368	asymptomatic patients	348:368	asymptomatic patients	348:368	We recently found enrichment of genes associated with calcification in carotid plaques from asymptomatic patients.
31109707	6	74	from	patients	931:938	arg1	arteries					868:875	arteries	868:875	arteries with medial calcification from chronic kidney disease patients	868:938	Immunohistochemistry and qPCR were performed to evaluate the findings in plaques and arteries with medial calcification from chronic kidney disease patients.
31109707	6	74	from	patients	931:938	arg1	plaques					856:862	plaques	856:862	plaques	856:862	Immunohistochemistry and qPCR were performed to evaluate the findings in plaques and arteries with medial calcification from chronic kidney disease patients.
31109707	6	74	from	patients	931:938	arg1	findings					844:851	the findings	840:851	the findings in plaques and arteries with medial calcification from chronic kidney disease patients	840:938	Immunohistochemistry and qPCR were performed to evaluate the findings in plaques and arteries with medial calcification from chronic kidney disease patients.
31109707	10	75	with	association	1460:1470	arg1	calcification					1484:1496	plaque calcification	1477:1496	plaque calcification	1477:1496	Proteoglycan 4 (PRG4) was identified as the most upregulated gene in association with plaque calcification and found in the ECM, SMA+ and CD68+/TRAP + cells.
31109707	3	76	theme	expression	561:570	arg1	profiles					572:579	gene expression profiles	556:579	gene expression profiles in lesions	556:590	Here, we hypothesized that calcification represents a stabilising feature of plaques and investigated how macro-calcification, as estimated by computed tomography (CT), correlates with gene expression profiles in lesions.
31109707	3	77	from	profiles	572:579	arg1	lesions					584:590	lesions	584:590	lesions	584:590	Here, we hypothesized that calcification represents a stabilising feature of plaques and investigated how macro-calcification, as estimated by computed tomography (CT), correlates with gene expression profiles in lesions.
31674784	9	0	theme	Caco-2	961:966	arg1	cells					968:972	Caco-2 cells	961:972	Caco-2 cells	961:972	Lastly, Caco-2 cells were used to confirm the protective effects of l-arabinose in colitis or inflammatory bowel disease.
31674784	11	1	theme	DSS-induced	1328:1338	arg1	colitis					1340:1346	DSS-induced colitis	1328:1346	DSS-induced colitis	1328:1346	Our work suggested that l-arabinose exerts anti-inflammation effects in DSS-induced colitis.
31674784	5	2	theme	16S-rRNA	498:505	arg1	analysis					516:523	16S-rRNA sequence analysis	498:523	16S-rRNA sequence analysis	498:523	16S-rRNA sequence analysis was performed to investigate gut microbiota.
31674784	10	3	theme	pro-inflammatory	1137:1152	arg1	genes					1154:1158	pro-inflammatory genes	1137:1158	pro-inflammatory genes	1137:1158	As expected, the protein expression levels in Caco-2 cells of pro-inflammatory genes, which were treated with l-arabinose and incubated with or without tumor necrosis factor alpha.
31674784	4	4	theme	genes	477:481	arg1	analysis					417:424	The histological analysis	400:424	The histological analysis	400:424	The histological analysis, disease index, and the expression of inflammatory genes were measured.
31674784	4	4	theme	genes	477:481	arg1	index					435:439	disease index	427:439	disease index	427:439	The histological analysis, disease index, and the expression of inflammatory genes were measured.
31674784	4	4	theme	genes	477:481	arg1	expression					450:459	the expression	446:459	the expression of inflammatory genes	446:481	The histological analysis, disease index, and the expression of inflammatory genes were measured.
31674784	13	5	theme	therapeutic	1561:1571	arg1	strategy					1573:1580	a functional food or novel therapeutic strategy	1534:1580	strategy	1573:1580	l-Arabinose could be a remarkable candidate as a functional food or novel therapeutic strategy for intestinal health.
31674784	10	6	theme	factor	1242:1247	arg1	alpha					1249:1253	tumor necrosis factor alpha	1227:1253	tumor necrosis factor alpha	1227:1253	As expected, the protein expression levels in Caco-2 cells of pro-inflammatory genes, which were treated with l-arabinose and incubated with or without tumor necrosis factor alpha.
31674784	4	7	theme	inflammatory	464:475	arg1	genes					477:481	inflammatory genes	464:481	inflammatory genes	464:481	The histological analysis, disease index, and the expression of inflammatory genes were measured.
31674784	12	8	theme	microbiota	1450:1459	arg1	diversity					1414:1422	diversity	1414:1422	diversity	1414:1422	These beneficial effects have correlations with the composition, diversity, and abundance of the gut microbiota regulated by l-arabinose.
31674784	12	8	theme	microbiota	1450:1459	arg1	abundance					1429:1437	abundance	1429:1437	abundance	1429:1437	These beneficial effects have correlations with the composition, diversity, and abundance of the gut microbiota regulated by l-arabinose.
31674784	12	8	theme	microbiota	1450:1459	arg1	composition					1401:1411	composition	1401:1411	composition	1401:1411	These beneficial effects have correlations with the composition, diversity, and abundance of the gut microbiota regulated by l-arabinose.
31674784	8	9	theme	gut	830:832	arg1	responsible					849:859	responsible	849:859	responsible	849:859	Additionally, the perturbed gut microbiota was responsible for the suppressive effects of l-arabinose on DSS-induced colitis treated with antibiotics.
31674784	8	9	theme	gut	830:832	arg1	microbiota					834:843	the perturbed gut microbiota	816:843	the perturbed gut microbiota	816:843	Additionally, the perturbed gut microbiota was responsible for the suppressive effects of l-arabinose on DSS-induced colitis treated with antibiotics.
31674784	9	10	from	effects	1010:1016	arg1	disease					1066:1072	inflammatory bowel disease	1047:1072	inflammatory bowel disease	1047:1072	Lastly, Caco-2 cells were used to confirm the protective effects of l-arabinose in colitis or inflammatory bowel disease.
31674784	9	10	from	effects	1010:1016	arg1	colitis					1036:1042	colitis	1036:1042	colitis	1036:1042	Lastly, Caco-2 cells were used to confirm the protective effects of l-arabinose in colitis or inflammatory bowel disease.
31674784	12	11	with	correlations	1379:1390	arg1	diversity					1414:1422	diversity	1414:1422	diversity	1414:1422	These beneficial effects have correlations with the composition, diversity, and abundance of the gut microbiota regulated by l-arabinose.
31674784	12	11	with	correlations	1379:1390	arg1	abundance					1429:1437	abundance	1429:1437	abundance	1429:1437	These beneficial effects have correlations with the composition, diversity, and abundance of the gut microbiota regulated by l-arabinose.
31674784	12	11	with	correlations	1379:1390	arg1	composition					1401:1411	composition	1401:1411	composition	1401:1411	These beneficial effects have correlations with the composition, diversity, and abundance of the gut microbiota regulated by l-arabinose.
31674784	12	12	theme	gut	1446:1448	arg1	microbiota					1450:1459	the gut microbiota	1442:1459	the gut microbiota regulated by l-arabinose	1442:1484	These beneficial effects have correlations with the composition, diversity, and abundance of the gut microbiota regulated by l-arabinose.
31674784	10	13	theme	expression	1100:1109	arg1	levels					1111:1116	the protein expression levels	1088:1116	the protein expression levels in Caco-2 cells of pro-inflammatory genes, which were treated with l-arabinose and incubated with or without tumor necrosis factor alpha	1088:1253	As expected, the protein expression levels in Caco-2 cells of pro-inflammatory genes, which were treated with l-arabinose and incubated with or without tumor necrosis factor alpha.
31674784	8	14	theme	perturbed	820:828	arg1	responsible					849:859	responsible	849:859	responsible	849:859	Additionally, the perturbed gut microbiota was responsible for the suppressive effects of l-arabinose on DSS-induced colitis treated with antibiotics.
31674784	8	14	theme	perturbed	820:828	arg1	microbiota					834:843	the perturbed gut microbiota	816:843	the perturbed gut microbiota	816:843	Additionally, the perturbed gut microbiota was responsible for the suppressive effects of l-arabinose on DSS-induced colitis treated with antibiotics.
31674784	0	15	from	Microbiota	47:56	arg1	Mice					61:64	Mice	61:64	Mice	61:64	l-Arabinose Inhibits Colitis by Modulating Gut Microbiota in Mice.
31674784	8	16	theme	l-arabinose	892:902	arg1	effects					881:887	the suppressive effects	865:887	the suppressive effects of l-arabinose on DSS-induced colitis treated with antibiotics	865:950	Additionally, the perturbed gut microbiota was responsible for the suppressive effects of l-arabinose on DSS-induced colitis treated with antibiotics.
31674784	10	17	theme	tumor	1227:1231	arg1	alpha					1249:1253	tumor necrosis factor alpha	1227:1253	tumor necrosis factor alpha	1227:1253	As expected, the protein expression levels in Caco-2 cells of pro-inflammatory genes, which were treated with l-arabinose and incubated with or without tumor necrosis factor alpha.
31674784	9	18	theme	l-arabinose	1021:1031	arg1	effects					1010:1016	the protective effects	995:1016	the protective effects of l-arabinose in colitis or inflammatory bowel disease	995:1072	Lastly, Caco-2 cells were used to confirm the protective effects of l-arabinose in colitis or inflammatory bowel disease.
31674784	13	19	theme	novel	1555:1559	arg1	strategy					1573:1580	a functional food or novel therapeutic strategy	1534:1580	strategy	1573:1580	l-Arabinose could be a remarkable candidate as a functional food or novel therapeutic strategy for intestinal health.
31674784	10	20	theme	Caco-2	1121:1126	arg1	cells					1128:1132	Caco-2 cells	1121:1132	Caco-2 cells	1121:1132	As expected, the protein expression levels in Caco-2 cells of pro-inflammatory genes, which were treated with l-arabinose and incubated with or without tumor necrosis factor alpha.
31674784	8	21	theme	DSS-induced	907:917	arg1	colitis					919:925	DSS-induced colitis	907:925	DSS-induced colitis treated with antibiotics	907:950	Additionally, the perturbed gut microbiota was responsible for the suppressive effects of l-arabinose on DSS-induced colitis treated with antibiotics.
31674784	3	22	theme	colitis	385:391	arg1	model					393:397	a dextran sodium sulfate (DSS)-induced colitis model	346:397	a dextran sodium sulfate (DSS)-induced colitis model	346:397	l-Arabinose was administered in mice daily in a dextran sodium sulfate (DSS)-induced colitis model.
31674784	9	23	theme	protective	999:1008	arg1	effects					1010:1016	the protective effects	995:1016	the protective effects of l-arabinose in colitis or inflammatory bowel disease	995:1072	Lastly, Caco-2 cells were used to confirm the protective effects of l-arabinose in colitis or inflammatory bowel disease.
31674784	5	24	theme	gut	554:556	arg1	microbiota					558:567	gut microbiota	554:567	gut microbiota	554:567	16S-rRNA sequence analysis was performed to investigate gut microbiota.
31674784	2	25	theme	gut	284:286	arg1	microbiota					288:297	gut microbiota	284:297	gut microbiota	284:297	In this study, we aim to investigate whether l-arabinose could inhibit colitis by modulating gut microbiota.
31674784	12	26	theme	beneficial	1355:1364	arg1	effects					1366:1372	These beneficial effects	1349:1372	These beneficial effects	1349:1372	These beneficial effects have correlations with the composition, diversity, and abundance of the gut microbiota regulated by l-arabinose.
31674784	12	27	contain	have	1374:1377	arg2	correlations					1379:1390	correlations	1379:1390	correlations with the composition, diversity, and abundance of the gut microbiota regulated by l-arabinose	1379:1484	These beneficial effects have correlations with the composition, diversity, and abundance of the gut microbiota regulated by l-arabinose.
31674784	12	27	contain	have	1374:1377	arg1	effects					1366:1372	These beneficial effects	1349:1372	These beneficial effects	1349:1372	These beneficial effects have correlations with the composition, diversity, and abundance of the gut microbiota regulated by l-arabinose.
31674784	6	28	theme	inflammation	675:686	arg1	activation					688:697	p38-/p65-dependent inflammation activation	656:697	p38-/p65-dependent inflammation activation	656:697	Intriguingly, we found that l-arabinose could repress DSS-induced colitis and inhibit p38-/p65-dependent inflammation activation.
31674784	1	29	theme	functional	168:177	arg1	properties					179:188	functional properties	168:188	functional properties	168:188	l-Arabinose is a monosaccharide extracted from plants or fibers, which is known to have a variety of functional properties.
31674784	8	30	from	effects	881:887	arg1	colitis					919:925	DSS-induced colitis	907:925	DSS-induced colitis treated with antibiotics	907:950	Additionally, the perturbed gut microbiota was responsible for the suppressive effects of l-arabinose on DSS-induced colitis treated with antibiotics.
31674784	6	31	theme	p38-/p65-dependent	656:673	arg1	activation					688:697	p38-/p65-dependent inflammation activation	656:697	p38-/p65-dependent inflammation activation	656:697	Intriguingly, we found that l-arabinose could repress DSS-induced colitis and inhibit p38-/p65-dependent inflammation activation.
31674784	1	32	theme	properties	179:188	arg1	variety					157:163	a variety	155:163	a variety of functional properties	155:188	l-Arabinose is a monosaccharide extracted from plants or fibers, which is known to have a variety of functional properties.
31674784	1	32	theme	properties	179:188	arg1	properties					179:188	functional properties	168:188	functional properties	168:188	l-Arabinose is a monosaccharide extracted from plants or fibers, which is known to have a variety of functional properties.
31674784	0	33	theme	Gut	43:45	arg1	Microbiota					47:56	Gut Microbiota	43:56	Gut Microbiota in Mice	43:64	l-Arabinose Inhibits Colitis by Modulating Gut Microbiota in Mice.
31674784	10	34	theme	genes	1154:1158	arg1	cells					1128:1132	Caco-2 cells	1121:1132	Caco-2 cells	1121:1132	As expected, the protein expression levels in Caco-2 cells of pro-inflammatory genes, which were treated with l-arabinose and incubated with or without tumor necrosis factor alpha.
31674784	4	35	theme	histological	404:415	arg1	analysis					417:424	The histological analysis	400:424	The histological analysis	400:424	The histological analysis, disease index, and the expression of inflammatory genes were measured.
31674784	13	36	theme	functional	1536:1545	arg1	food					1547:1550	a functional food or novel therapeutic strategy	1534:1580	food	1547:1550	l-Arabinose could be a remarkable candidate as a functional food or novel therapeutic strategy for intestinal health.
31674784	3	37	theme	-induced	376:383	arg1	model					393:397	a dextran sodium sulfate (DSS)-induced colitis model	346:397	a dextran sodium sulfate (DSS)-induced colitis model	346:397	l-Arabinose was administered in mice daily in a dextran sodium sulfate (DSS)-induced colitis model.
31674784	4	38	theme	disease	427:433	arg1	index					435:439	disease index	427:439	disease index	427:439	The histological analysis, disease index, and the expression of inflammatory genes were measured.
31674784	9	39	used	used	979:982	arg2	cells					968:972	Caco-2 cells	961:972	Caco-2 cells	961:972	Lastly, Caco-2 cells were used to confirm the protective effects of l-arabinose in colitis or inflammatory bowel disease.
31674784	5	40	theme	sequence	507:514	arg1	analysis					516:523	16S-rRNA sequence analysis	498:523	16S-rRNA sequence analysis	498:523	16S-rRNA sequence analysis was performed to investigate gut microbiota.
31674784	1	41	contain	have	150:153	arg1	l-Arabinose					67:77	l-Arabinose	67:77	l-Arabinose	67:77	l-Arabinose is a monosaccharide extracted from plants or fibers, which is known to have a variety of functional properties.
31674784	1	41	contain	have	150:153	arg1	monosaccharide					84:97	a monosaccharide	82:97	a monosaccharide	82:97	l-Arabinose is a monosaccharide extracted from plants or fibers, which is known to have a variety of functional properties.
31674784	1	41	contain	have	150:153	arg2	properties					179:188	functional properties	168:188	functional properties	168:188	l-Arabinose is a monosaccharide extracted from plants or fibers, which is known to have a variety of functional properties.
31674784	1	41	contain	have	150:153	arg2	variety					157:163	a variety	155:163	a variety of functional properties	155:188	l-Arabinose is a monosaccharide extracted from plants or fibers, which is known to have a variety of functional properties.
31674784	10	42	theme	protein	1092:1098	arg1	levels					1111:1116	the protein expression levels	1088:1116	the protein expression levels in Caco-2 cells of pro-inflammatory genes, which were treated with l-arabinose and incubated with or without tumor necrosis factor alpha	1088:1253	As expected, the protein expression levels in Caco-2 cells of pro-inflammatory genes, which were treated with l-arabinose and incubated with or without tumor necrosis factor alpha.
31674784	11	43	theme	anti-inflammation	1299:1315	arg1	effects					1317:1323	anti-inflammation effects	1299:1323	anti-inflammation effects	1299:1323	Our work suggested that l-arabinose exerts anti-inflammation effects in DSS-induced colitis.
31674784	9	44	theme	inflammatory	1047:1058	arg1	disease					1066:1072	inflammatory bowel disease	1047:1072	inflammatory bowel disease	1047:1072	Lastly, Caco-2 cells were used to confirm the protective effects of l-arabinose in colitis or inflammatory bowel disease.
31674784	13	45	theme	remarkable	1510:1519	arg1	l-Arabinose					1487:1497	l-Arabinose	1487:1497	l-Arabinose	1487:1497	l-Arabinose could be a remarkable candidate as a functional food or novel therapeutic strategy for intestinal health.
31674784	13	45	theme	remarkable	1510:1519	arg1	candidate					1521:1529	a remarkable candidate	1508:1529	a remarkable candidate as a functional food or novel therapeutic strategy for intestinal health	1508:1602	l-Arabinose could be a remarkable candidate as a functional food or novel therapeutic strategy for intestinal health.
31674784	9	46	theme	bowel	1060:1064	arg1	disease					1066:1072	inflammatory bowel disease	1047:1072	inflammatory bowel disease	1047:1072	Lastly, Caco-2 cells were used to confirm the protective effects of l-arabinose in colitis or inflammatory bowel disease.
31674784	7	47	theme	gut	771:773	arg1	microbiota					775:784	l-arabinose-modulated DSS-induced gut microbiota	737:784	l-arabinose-modulated DSS-induced gut microbiota	737:784	Besides that, our data revealed that l-arabinose-modulated DSS-induced gut microbiota were disturbed.
31674784	13	48	theme	intestinal	1586:1595	arg1	health					1597:1602	intestinal health	1586:1602	intestinal health	1586:1602	l-Arabinose could be a remarkable candidate as a functional food or novel therapeutic strategy for intestinal health.
31674784	2	49	from	aim	209:211	arg1	study					199:203	this study	194:203	this study	194:203	In this study, we aim to investigate whether l-arabinose could inhibit colitis by modulating gut microbiota.
31674784	6	50	theme	DSS-induced	624:634	arg1	colitis					636:642	DSS-induced colitis	624:642	DSS-induced colitis	624:642	Intriguingly, we found that l-arabinose could repress DSS-induced colitis and inhibit p38-/p65-dependent inflammation activation.
31674784	7	51	theme	l-arabinose-modulated	737:757	arg1	microbiota					775:784	l-arabinose-modulated DSS-induced gut microbiota	737:784	l-arabinose-modulated DSS-induced gut microbiota	737:784	Besides that, our data revealed that l-arabinose-modulated DSS-induced gut microbiota were disturbed.
31674784	10	52	theme	necrosis	1233:1240	arg1	alpha					1249:1253	tumor necrosis factor alpha	1227:1253	tumor necrosis factor alpha	1227:1253	As expected, the protein expression levels in Caco-2 cells of pro-inflammatory genes, which were treated with l-arabinose and incubated with or without tumor necrosis factor alpha.
31674784	10	53	from	levels	1111:1116	arg1	cells					1128:1132	Caco-2 cells	1121:1132	Caco-2 cells	1121:1132	As expected, the protein expression levels in Caco-2 cells of pro-inflammatory genes, which were treated with l-arabinose and incubated with or without tumor necrosis factor alpha.
31674784	10	54	dep	expected	1078:1085	arg1	levels					1111:1116	the protein expression levels	1088:1116	the protein expression levels in Caco-2 cells of pro-inflammatory genes, which were treated with l-arabinose and incubated with or without tumor necrosis factor alpha	1088:1253	As expected, the protein expression levels in Caco-2 cells of pro-inflammatory genes, which were treated with l-arabinose and incubated with or without tumor necrosis factor alpha.
31674784	8	55	theme	suppressive	869:879	arg1	effects					881:887	the suppressive effects	865:887	the suppressive effects of l-arabinose on DSS-induced colitis treated with antibiotics	865:950	Additionally, the perturbed gut microbiota was responsible for the suppressive effects of l-arabinose on DSS-induced colitis treated with antibiotics.
31674784	7	56	theme	DSS-induced	759:769	arg1	microbiota					775:784	l-arabinose-modulated DSS-induced gut microbiota	737:784	l-arabinose-modulated DSS-induced gut microbiota	737:784	Besides that, our data revealed that l-arabinose-modulated DSS-induced gut microbiota were disturbed.
30282906	2	0	theme	C57BL/6J	488:495	arg1	mice					497:500	Nine-week-old male C57BL/6J mice	469:500	Nine-week-old male C57BL/6J mice	469:500	Nine-week-old male C57BL/6J mice were divided into four groups: group 1 received normal diet, whereas groups 2, 3 and 4 received normal diet containing 0.3% paramylon, 1.0% Euglena, or 1.0% Euglena plus 0.3% vegetables (barley leaf, kale and ashitaba), respectively.
30282906	7	1	theme	genes	1369:1373	arg1	expression					1355:1364	the expression	1351:1364	the expression of genes related to adipocyte growth and lipolysis	1351:1415	Subsequent analyses showed that co-consumption of Euglena with vegetables reduced adipocyte area, suppressed the expression of genes related to fatty acid synthesis and increased the expression of genes related to adipocyte growth and lipolysis.
30282906	1	2	from	alga	325:328	arg1	paramylon					366:374	insoluble dietary fiber paramylon	342:374	insoluble dietary fiber paramylon	342:374	We determined whether the benefits provided by the consumption of Euglena gracilis (Euglena), which is a unicellular photosynthesizing green alga and rich in insoluble dietary fiber paramylon, can be enhanced by the co-consumption of vegetables that are rich in soluble dietary fiber.
30282906	2	3	theme	%	657:657	arg1	Euglena					659:665	1.0% Euglena	654:665	1.0% Euglena	654:665	Nine-week-old male C57BL/6J mice were divided into four groups: group 1 received normal diet, whereas groups 2, 3 and 4 received normal diet containing 0.3% paramylon, 1.0% Euglena, or 1.0% Euglena plus 0.3% vegetables (barley leaf, kale and ashitaba), respectively.
30282906	0	4	theme	Anti-Inflammatory	81:97	arg1	Effect					99:104	an Anti-Inflammatory Effect	78:104	an Anti-Inflammatory Effect	78:104	Simultaneous Intake of Euglena Gracilis and Vegetables Synergistically Exerts an Anti-Inflammatory Effect and Attenuates Visceral Fat Accumulation by Affecting Gut Microbiota in Mice.
30282906	1	5	theme	insoluble	342:350	arg1	paramylon					366:374	insoluble dietary fiber paramylon	342:374	insoluble dietary fiber paramylon	342:374	We determined whether the benefits provided by the consumption of Euglena gracilis (Euglena), which is a unicellular photosynthesizing green alga and rich in insoluble dietary fiber paramylon, can be enhanced by the co-consumption of vegetables that are rich in soluble dietary fiber.
30282906	5	6	theme	beneficial	963:972	arg1	bacteria					974:981	beneficial bacteria	963:981	beneficial bacteria producing short-chain fatty acids	963:1015	Notably, we observed an increase in the fraction of beneficial bacteria producing short-chain fatty acids, a reduction in harmful bacteria that cause inflammation and an increase in short-chain fatty acid production.
30282906	2	7	theme	%	624:624	arg1	paramylon					626:634	0.3% paramylon	621:634	0.3% paramylon	621:634	Nine-week-old male C57BL/6J mice were divided into four groups: group 1 received normal diet, whereas groups 2, 3 and 4 received normal diet containing 0.3% paramylon, 1.0% Euglena, or 1.0% Euglena plus 0.3% vegetables (barley leaf, kale and ashitaba), respectively.
30282906	1	8	from	rich	438:441	arg1	fiber					462:466	soluble dietary fiber	446:466	soluble dietary fiber	446:466	We determined whether the benefits provided by the consumption of Euglena gracilis (Euglena), which is a unicellular photosynthesizing green alga and rich in insoluble dietary fiber paramylon, can be enhanced by the co-consumption of vegetables that are rich in soluble dietary fiber.
30282906	8	9	from	effect	1528:1533	arg1	accumulation					1551:1562	visceral fat accumulation	1538:1562	visceral fat accumulation	1538:1562	Therefore, co-consumption of Euglena with vegetables enhanced its anti-inflammatory effect and the inhibitory effect on visceral fat accumulation likely by modulating the composition of gut microbiota.
30282906	1	10	theme	dietary	352:358	arg1	paramylon					366:374	insoluble dietary fiber paramylon	342:374	insoluble dietary fiber paramylon	342:374	We determined whether the benefits provided by the consumption of Euglena gracilis (Euglena), which is a unicellular photosynthesizing green alga and rich in insoluble dietary fiber paramylon, can be enhanced by the co-consumption of vegetables that are rich in soluble dietary fiber.
30282906	2	11	theme	0.3	621:623	arg1	%					624:624	%	624:624	%	624:624	Nine-week-old male C57BL/6J mice were divided into four groups: group 1 received normal diet, whereas groups 2, 3 and 4 received normal diet containing 0.3% paramylon, 1.0% Euglena, or 1.0% Euglena plus 0.3% vegetables (barley leaf, kale and ashitaba), respectively.
30282906	7	12	theme	related	1375:1381	arg1	genes					1369:1373	genes	1369:1373	genes related to adipocyte growth and lipolysis	1369:1415	Subsequent analyses showed that co-consumption of Euglena with vegetables reduced adipocyte area, suppressed the expression of genes related to fatty acid synthesis and increased the expression of genes related to adipocyte growth and lipolysis.
30282906	8	13	theme	microbiota	1608:1617	arg1	composition					1589:1599	the composition	1585:1599	the composition of gut microbiota	1585:1617	Therefore, co-consumption of Euglena with vegetables enhanced its anti-inflammatory effect and the inhibitory effect on visceral fat accumulation likely by modulating the composition of gut microbiota.
30282906	2	14	dep	vegetables	677:686	arg1	ashitaba					711:718	ashitaba	711:718	ashitaba	711:718	Nine-week-old male C57BL/6J mice were divided into four groups: group 1 received normal diet, whereas groups 2, 3 and 4 received normal diet containing 0.3% paramylon, 1.0% Euglena, or 1.0% Euglena plus 0.3% vegetables (barley leaf, kale and ashitaba), respectively.
30282906	2	14	dep	vegetables	677:686	arg1	kale					702:705	kale	702:705	kale	702:705	Nine-week-old male C57BL/6J mice were divided into four groups: group 1 received normal diet, whereas groups 2, 3 and 4 received normal diet containing 0.3% paramylon, 1.0% Euglena, or 1.0% Euglena plus 0.3% vegetables (barley leaf, kale and ashitaba), respectively.
30282906	2	14	dep	vegetables	677:686	arg1	vegetables					677:686	0.3% vegetables	672:686	0.3% vegetables (barley leaf, kale and ashitaba)	672:719	Nine-week-old male C57BL/6J mice were divided into four groups: group 1 received normal diet, whereas groups 2, 3 and 4 received normal diet containing 0.3% paramylon, 1.0% Euglena, or 1.0% Euglena plus 0.3% vegetables (barley leaf, kale and ashitaba), respectively.
30282906	2	14	dep	vegetables	677:686	arg1	leaf					696:699	barley leaf	689:699	barley leaf	689:699	Nine-week-old male C57BL/6J mice were divided into four groups: group 1 received normal diet, whereas groups 2, 3 and 4 received normal diet containing 0.3% paramylon, 1.0% Euglena, or 1.0% Euglena plus 0.3% vegetables (barley leaf, kale and ashitaba), respectively.
30282906	1	15	theme	fiber	360:364	arg1	paramylon					366:374	insoluble dietary fiber paramylon	342:374	insoluble dietary fiber paramylon	342:374	We determined whether the benefits provided by the consumption of Euglena gracilis (Euglena), which is a unicellular photosynthesizing green alga and rich in insoluble dietary fiber paramylon, can be enhanced by the co-consumption of vegetables that are rich in soluble dietary fiber.
30282906	8	16	theme	gut	1604:1606	arg1	microbiota					1608:1617	gut microbiota	1604:1617	gut microbiota	1604:1617	Therefore, co-consumption of Euglena with vegetables enhanced its anti-inflammatory effect and the inhibitory effect on visceral fat accumulation likely by modulating the composition of gut microbiota.
30282906	2	17	theme	barley	689:694	arg1	vegetables					677:686	0.3% vegetables	672:686	0.3% vegetables (barley leaf, kale and ashitaba)	672:719	Nine-week-old male C57BL/6J mice were divided into four groups: group 1 received normal diet, whereas groups 2, 3 and 4 received normal diet containing 0.3% paramylon, 1.0% Euglena, or 1.0% Euglena plus 0.3% vegetables (barley leaf, kale and ashitaba), respectively.
30282906	2	17	theme	barley	689:694	arg1	leaf					696:699	barley leaf	689:699	barley leaf	689:699	Nine-week-old male C57BL/6J mice were divided into four groups: group 1 received normal diet, whereas groups 2, 3 and 4 received normal diet containing 0.3% paramylon, 1.0% Euglena, or 1.0% Euglena plus 0.3% vegetables (barley leaf, kale and ashitaba), respectively.
30282906	0	18	from	Microbiota	164:173	arg1	Mice					178:181	Mice	178:181	Mice	178:181	Simultaneous Intake of Euglena Gracilis and Vegetables Synergistically Exerts an Anti-Inflammatory Effect and Attenuates Visceral Fat Accumulation by Affecting Gut Microbiota in Mice.
30282906	0	19	theme	Fat	130:132	arg1	Accumulation					134:145	Visceral Fat Accumulation	121:145	Visceral Fat Accumulation	121:145	Simultaneous Intake of Euglena Gracilis and Vegetables Synergistically Exerts an Anti-Inflammatory Effect and Attenuates Visceral Fat Accumulation by Affecting Gut Microbiota in Mice.
30282906	2	20	theme	normal	598:603	arg1	diet					605:608	normal diet	598:608	normal diet containing 0.3% paramylon, 1.0% Euglena, or 1.0% Euglena	598:665	Nine-week-old male C57BL/6J mice were divided into four groups: group 1 received normal diet, whereas groups 2, 3 and 4 received normal diet containing 0.3% paramylon, 1.0% Euglena, or 1.0% Euglena plus 0.3% vegetables (barley leaf, kale and ashitaba), respectively.
30282906	1	21	theme	soluble	446:452	arg1	fiber					462:466	soluble dietary fiber	446:466	soluble dietary fiber	446:466	We determined whether the benefits provided by the consumption of Euglena gracilis (Euglena), which is a unicellular photosynthesizing green alga and rich in insoluble dietary fiber paramylon, can be enhanced by the co-consumption of vegetables that are rich in soluble dietary fiber.
30282906	0	22	theme	Visceral	121:128	arg1	Accumulation					134:145	Visceral Fat Accumulation	121:145	Visceral Fat Accumulation	121:145	Simultaneous Intake of Euglena Gracilis and Vegetables Synergistically Exerts an Anti-Inflammatory Effect and Attenuates Visceral Fat Accumulation by Affecting Gut Microbiota in Mice.
30282906	7	23	theme	adipocyte	1386:1394	arg1	growth					1396:1401	adipocyte growth	1386:1401	adipocyte growth	1386:1401	Subsequent analyses showed that co-consumption of Euglena with vegetables reduced adipocyte area, suppressed the expression of genes related to fatty acid synthesis and increased the expression of genes related to adipocyte growth and lipolysis.
30282906	7	24	with	co-consumption	1204:1217	arg1	vegetables					1235:1244	vegetables	1235:1244	vegetables	1235:1244	Subsequent analyses showed that co-consumption of Euglena with vegetables reduced adipocyte area, suppressed the expression of genes related to fatty acid synthesis and increased the expression of genes related to adipocyte growth and lipolysis.
30282906	1	25	theme	dietary	454:460	arg1	fiber					462:466	soluble dietary fiber	446:466	soluble dietary fiber	446:466	We determined whether the benefits provided by the consumption of Euglena gracilis (Euglena), which is a unicellular photosynthesizing green alga and rich in insoluble dietary fiber paramylon, can be enhanced by the co-consumption of vegetables that are rich in soluble dietary fiber.
30282906	2	26	contain	containing	610:619	arg2	Euglena					642:648	1.0% Euglena	637:648	1.0% Euglena	637:648	Nine-week-old male C57BL/6J mice were divided into four groups: group 1 received normal diet, whereas groups 2, 3 and 4 received normal diet containing 0.3% paramylon, 1.0% Euglena, or 1.0% Euglena plus 0.3% vegetables (barley leaf, kale and ashitaba), respectively.
30282906	2	26	contain	containing	610:619	arg1	diet					605:608	normal diet	598:608	normal diet containing 0.3% paramylon, 1.0% Euglena, or 1.0% Euglena	598:665	Nine-week-old male C57BL/6J mice were divided into four groups: group 1 received normal diet, whereas groups 2, 3 and 4 received normal diet containing 0.3% paramylon, 1.0% Euglena, or 1.0% Euglena plus 0.3% vegetables (barley leaf, kale and ashitaba), respectively.
30282906	2	26	contain	containing	610:619	arg2	Euglena					659:665	1.0% Euglena	654:665	1.0% Euglena	654:665	Nine-week-old male C57BL/6J mice were divided into four groups: group 1 received normal diet, whereas groups 2, 3 and 4 received normal diet containing 0.3% paramylon, 1.0% Euglena, or 1.0% Euglena plus 0.3% vegetables (barley leaf, kale and ashitaba), respectively.
30282906	2	26	contain	containing	610:619	arg2	paramylon					626:634	0.3% paramylon	621:634	0.3% paramylon	621:634	Nine-week-old male C57BL/6J mice were divided into four groups: group 1 received normal diet, whereas groups 2, 3 and 4 received normal diet containing 0.3% paramylon, 1.0% Euglena, or 1.0% Euglena plus 0.3% vegetables (barley leaf, kale and ashitaba), respectively.
30282906	4	27	theme	inflammation	840:851	arg1	markers					829:835	serum markers	823:835	serum markers of inflammation	823:851	Euglena intake significantly decreased serum markers of inflammation and co-consumption of vegetables enhanced this reduction.
30282906	2	28	dep	received	541:548	arg1	whereas					563:569	whereas	563:569	whereas	563:569	Nine-week-old male C57BL/6J mice were divided into four groups: group 1 received normal diet, whereas groups 2, 3 and 4 received normal diet containing 0.3% paramylon, 1.0% Euglena, or 1.0% Euglena plus 0.3% vegetables (barley leaf, kale and ashitaba), respectively.
30282906	5	29	from	reduction	1020:1028	arg1	bacteria					1041:1048	harmful bacteria	1033:1048	harmful bacteria that cause inflammation	1033:1072	Notably, we observed an increase in the fraction of beneficial bacteria producing short-chain fatty acids, a reduction in harmful bacteria that cause inflammation and an increase in short-chain fatty acid production.
30282906	5	29	from	reduction	1020:1028	arg1	production					1116:1125	short-chain fatty acid production	1093:1125	short-chain fatty acid production	1093:1125	Notably, we observed an increase in the fraction of beneficial bacteria producing short-chain fatty acids, a reduction in harmful bacteria that cause inflammation and an increase in short-chain fatty acid production.
30282906	5	29	from	reduction	1020:1028	arg1	fraction					951:958	the fraction	947:958	the fraction of beneficial bacteria producing short-chain fatty acids	947:1015	Notably, we observed an increase in the fraction of beneficial bacteria producing short-chain fatty acids, a reduction in harmful bacteria that cause inflammation and an increase in short-chain fatty acid production.
30282906	2	30	theme	male	483:486	arg1	mice					497:500	Nine-week-old male C57BL/6J mice	469:500	Nine-week-old male C57BL/6J mice	469:500	Nine-week-old male C57BL/6J mice were divided into four groups: group 1 received normal diet, whereas groups 2, 3 and 4 received normal diet containing 0.3% paramylon, 1.0% Euglena, or 1.0% Euglena plus 0.3% vegetables (barley leaf, kale and ashitaba), respectively.
30282906	2	31	theme	1.0	637:639	arg1	%					640:640	%	640:640	%	640:640	Nine-week-old male C57BL/6J mice were divided into four groups: group 1 received normal diet, whereas groups 2, 3 and 4 received normal diet containing 0.3% paramylon, 1.0% Euglena, or 1.0% Euglena plus 0.3% vegetables (barley leaf, kale and ashitaba), respectively.
30282906	2	32	theme	%	675:675	arg1	ashitaba					711:718	ashitaba	711:718	ashitaba	711:718	Nine-week-old male C57BL/6J mice were divided into four groups: group 1 received normal diet, whereas groups 2, 3 and 4 received normal diet containing 0.3% paramylon, 1.0% Euglena, or 1.0% Euglena plus 0.3% vegetables (barley leaf, kale and ashitaba), respectively.
30282906	2	32	theme	%	675:675	arg1	kale					702:705	kale	702:705	kale	702:705	Nine-week-old male C57BL/6J mice were divided into four groups: group 1 received normal diet, whereas groups 2, 3 and 4 received normal diet containing 0.3% paramylon, 1.0% Euglena, or 1.0% Euglena plus 0.3% vegetables (barley leaf, kale and ashitaba), respectively.
30282906	2	32	theme	%	675:675	arg1	vegetables					677:686	0.3% vegetables	672:686	0.3% vegetables (barley leaf, kale and ashitaba)	672:719	Nine-week-old male C57BL/6J mice were divided into four groups: group 1 received normal diet, whereas groups 2, 3 and 4 received normal diet containing 0.3% paramylon, 1.0% Euglena, or 1.0% Euglena plus 0.3% vegetables (barley leaf, kale and ashitaba), respectively.
30282906	2	32	theme	%	675:675	arg1	leaf					696:699	barley leaf	689:699	barley leaf	689:699	Nine-week-old male C57BL/6J mice were divided into four groups: group 1 received normal diet, whereas groups 2, 3 and 4 received normal diet containing 0.3% paramylon, 1.0% Euglena, or 1.0% Euglena plus 0.3% vegetables (barley leaf, kale and ashitaba), respectively.
30282906	0	33	theme	Simultaneous	0:11	arg1	Intake					13:18	Simultaneous Intake	0:18	Simultaneous Intake of Euglena Gracilis and Vegetables	0:53	Simultaneous Intake of Euglena Gracilis and Vegetables Synergistically Exerts an Anti-Inflammatory Effect and Attenuates Visceral Fat Accumulation by Affecting Gut Microbiota in Mice.
30282906	7	34	theme	genes	1299:1303	arg1	expression					1285:1294	the expression	1281:1294	the expression of genes related to fatty acid synthesis	1281:1335	Subsequent analyses showed that co-consumption of Euglena with vegetables reduced adipocyte area, suppressed the expression of genes related to fatty acid synthesis and increased the expression of genes related to adipocyte growth and lipolysis.
30282906	7	35	theme	related	1305:1311	arg1	genes					1299:1303	genes	1299:1303	genes related to fatty acid synthesis	1299:1335	Subsequent analyses showed that co-consumption of Euglena with vegetables reduced adipocyte area, suppressed the expression of genes related to fatty acid synthesis and increased the expression of genes related to adipocyte growth and lipolysis.
30282906	5	36	from	increase	1081:1088	arg1	bacteria					1041:1048	harmful bacteria	1033:1048	harmful bacteria that cause inflammation	1033:1072	Notably, we observed an increase in the fraction of beneficial bacteria producing short-chain fatty acids, a reduction in harmful bacteria that cause inflammation and an increase in short-chain fatty acid production.
30282906	5	36	from	increase	1081:1088	arg1	production					1116:1125	short-chain fatty acid production	1093:1125	short-chain fatty acid production	1093:1125	Notably, we observed an increase in the fraction of beneficial bacteria producing short-chain fatty acids, a reduction in harmful bacteria that cause inflammation and an increase in short-chain fatty acid production.
30282906	5	36	from	increase	1081:1088	arg1	fraction					951:958	the fraction	947:958	the fraction of beneficial bacteria producing short-chain fatty acids	947:1015	Notably, we observed an increase in the fraction of beneficial bacteria producing short-chain fatty acids, a reduction in harmful bacteria that cause inflammation and an increase in short-chain fatty acid production.
30282906	0	37	theme	Euglena	23:29	arg1	Gracilis					31:38	Euglena Gracilis	23:38	Euglena Gracilis	23:38	Simultaneous Intake of Euglena Gracilis and Vegetables Synergistically Exerts an Anti-Inflammatory Effect and Attenuates Visceral Fat Accumulation by Affecting Gut Microbiota in Mice.
30282906	5	38	theme	short-chain	1093:1103	arg1	acid					1111:1114	short-chain fatty acid	1093:1114	short-chain fatty acid production	1093:1125	Notably, we observed an increase in the fraction of beneficial bacteria producing short-chain fatty acids, a reduction in harmful bacteria that cause inflammation and an increase in short-chain fatty acid production.
30282906	8	39	theme	visceral	1538:1545	arg1	fat					1547:1549	visceral fat	1538:1549	visceral fat accumulation	1538:1562	Therefore, co-consumption of Euglena with vegetables enhanced its anti-inflammatory effect and the inhibitory effect on visceral fat accumulation likely by modulating the composition of gut microbiota.
30282906	8	40	theme	Euglena	1447:1453	arg1	co-consumption					1429:1442	co-consumption	1429:1442	co-consumption of Euglena with vegetables	1429:1469	Therefore, co-consumption of Euglena with vegetables enhanced its anti-inflammatory effect and the inhibitory effect on visceral fat accumulation likely by modulating the composition of gut microbiota.
30282906	5	41	theme	fatty	1105:1109	arg1	acid					1111:1114	short-chain fatty acid	1093:1114	short-chain fatty acid production	1093:1125	Notably, we observed an increase in the fraction of beneficial bacteria producing short-chain fatty acids, a reduction in harmful bacteria that cause inflammation and an increase in short-chain fatty acid production.
30282906	4	42	theme	serum	823:827	arg1	markers					829:835	serum markers	823:835	serum markers of inflammation	823:851	Euglena intake significantly decreased serum markers of inflammation and co-consumption of vegetables enhanced this reduction.
30282906	7	43	theme	Subsequent	1172:1181	arg1	analyses					1183:1190	Subsequent analyses	1172:1190	Subsequent analyses	1172:1190	Subsequent analyses showed that co-consumption of Euglena with vegetables reduced adipocyte area, suppressed the expression of genes related to fatty acid synthesis and increased the expression of genes related to adipocyte growth and lipolysis.
30282906	7	44	theme	adipocyte	1254:1262	arg1	area					1264:1267	adipocyte area	1254:1267	adipocyte area	1254:1267	Subsequent analyses showed that co-consumption of Euglena with vegetables reduced adipocyte area, suppressed the expression of genes related to fatty acid synthesis and increased the expression of genes related to adipocyte growth and lipolysis.
30282906	2	45	theme	Nine-week-old	469:481	arg1	mice					497:500	Nine-week-old male C57BL/6J mice	469:500	Nine-week-old male C57BL/6J mice	469:500	Nine-week-old male C57BL/6J mice were divided into four groups: group 1 received normal diet, whereas groups 2, 3 and 4 received normal diet containing 0.3% paramylon, 1.0% Euglena, or 1.0% Euglena plus 0.3% vegetables (barley leaf, kale and ashitaba), respectively.
30282906	1	46	theme	unicellular	289:299	arg1	gracilis					258:265	Euglena gracilis	250:265	Euglena gracilis (Euglena)	250:275	We determined whether the benefits provided by the consumption of Euglena gracilis (Euglena), which is a unicellular photosynthesizing green alga and rich in insoluble dietary fiber paramylon, can be enhanced by the co-consumption of vegetables that are rich in soluble dietary fiber.
30282906	1	46	theme	unicellular	289:299	arg1	alga					325:328	a unicellular photosynthesizing green alga	287:328	a unicellular photosynthesizing green alga	287:328	We determined whether the benefits provided by the consumption of Euglena gracilis (Euglena), which is a unicellular photosynthesizing green alga and rich in insoluble dietary fiber paramylon, can be enhanced by the co-consumption of vegetables that are rich in soluble dietary fiber.
30282906	0	47	theme	Gut	160:162	arg1	Microbiota					164:173	Gut Microbiota	160:173	Gut Microbiota in Mice	160:181	Simultaneous Intake of Euglena Gracilis and Vegetables Synergistically Exerts an Anti-Inflammatory Effect and Attenuates Visceral Fat Accumulation by Affecting Gut Microbiota in Mice.
30282906	5	48	theme	short-chain	993:1003	arg1	acids					1011:1015	short-chain fatty acids	993:1015	short-chain fatty acids	993:1015	Notably, we observed an increase in the fraction of beneficial bacteria producing short-chain fatty acids, a reduction in harmful bacteria that cause inflammation and an increase in short-chain fatty acid production.
30282906	7	49	theme	acid	1322:1325	arg1	synthesis					1327:1335	fatty acid synthesis	1316:1335	fatty acid synthesis	1316:1335	Subsequent analyses showed that co-consumption of Euglena with vegetables reduced adipocyte area, suppressed the expression of genes related to fatty acid synthesis and increased the expression of genes related to adipocyte growth and lipolysis.
30282906	5	50	from	increase	935:942	arg1	bacteria					1041:1048	harmful bacteria	1033:1048	harmful bacteria that cause inflammation	1033:1072	Notably, we observed an increase in the fraction of beneficial bacteria producing short-chain fatty acids, a reduction in harmful bacteria that cause inflammation and an increase in short-chain fatty acid production.
30282906	5	50	from	increase	935:942	arg1	production					1116:1125	short-chain fatty acid production	1093:1125	short-chain fatty acid production	1093:1125	Notably, we observed an increase in the fraction of beneficial bacteria producing short-chain fatty acids, a reduction in harmful bacteria that cause inflammation and an increase in short-chain fatty acid production.
30282906	5	50	from	increase	935:942	arg1	fraction					951:958	the fraction	947:958	the fraction of beneficial bacteria producing short-chain fatty acids	947:1015	Notably, we observed an increase in the fraction of beneficial bacteria producing short-chain fatty acids, a reduction in harmful bacteria that cause inflammation and an increase in short-chain fatty acid production.
30282906	0	51	theme	Gracilis	31:38	arg1	Intake					13:18	Simultaneous Intake	0:18	Simultaneous Intake of Euglena Gracilis and Vegetables	0:53	Simultaneous Intake of Euglena Gracilis and Vegetables Synergistically Exerts an Anti-Inflammatory Effect and Attenuates Visceral Fat Accumulation by Affecting Gut Microbiota in Mice.
30282906	1	52	theme	photosynthesizing	301:317	arg1	gracilis					258:265	Euglena gracilis	250:265	Euglena gracilis (Euglena)	250:275	We determined whether the benefits provided by the consumption of Euglena gracilis (Euglena), which is a unicellular photosynthesizing green alga and rich in insoluble dietary fiber paramylon, can be enhanced by the co-consumption of vegetables that are rich in soluble dietary fiber.
30282906	1	52	theme	photosynthesizing	301:317	arg1	alga					325:328	a unicellular photosynthesizing green alga	287:328	a unicellular photosynthesizing green alga	287:328	We determined whether the benefits provided by the consumption of Euglena gracilis (Euglena), which is a unicellular photosynthesizing green alga and rich in insoluble dietary fiber paramylon, can be enhanced by the co-consumption of vegetables that are rich in soluble dietary fiber.
30282906	2	53	dep	groups	571:576	arg1	4					587:587	4	587:587	4	587:587	Nine-week-old male C57BL/6J mice were divided into four groups: group 1 received normal diet, whereas groups 2, 3 and 4 received normal diet containing 0.3% paramylon, 1.0% Euglena, or 1.0% Euglena plus 0.3% vegetables (barley leaf, kale and ashitaba), respectively.
30282906	2	53	dep	groups	571:576	arg1	2					578:578	2	578:578	2	578:578	Nine-week-old male C57BL/6J mice were divided into four groups: group 1 received normal diet, whereas groups 2, 3 and 4 received normal diet containing 0.3% paramylon, 1.0% Euglena, or 1.0% Euglena plus 0.3% vegetables (barley leaf, kale and ashitaba), respectively.
30282906	5	54	theme	fatty	1005:1009	arg1	acids					1011:1015	short-chain fatty acids	993:1015	short-chain fatty acids	993:1015	Notably, we observed an increase in the fraction of beneficial bacteria producing short-chain fatty acids, a reduction in harmful bacteria that cause inflammation and an increase in short-chain fatty acid production.
30282906	2	55	theme	1.0	654:656	arg1	%					657:657	%	657:657	%	657:657	Nine-week-old male C57BL/6J mice were divided into four groups: group 1 received normal diet, whereas groups 2, 3 and 4 received normal diet containing 0.3% paramylon, 1.0% Euglena, or 1.0% Euglena plus 0.3% vegetables (barley leaf, kale and ashitaba), respectively.
30282906	2	56	dep	groups	525:530	arg1	received					541:548	received	541:548	received normal diet	541:560	Nine-week-old male C57BL/6J mice were divided into four groups: group 1 received normal diet, whereas groups 2, 3 and 4 received normal diet containing 0.3% paramylon, 1.0% Euglena, or 1.0% Euglena plus 0.3% vegetables (barley leaf, kale and ashitaba), respectively.
30282906	1	57	theme	green	319:323	arg1	gracilis					258:265	Euglena gracilis	250:265	Euglena gracilis (Euglena)	250:275	We determined whether the benefits provided by the consumption of Euglena gracilis (Euglena), which is a unicellular photosynthesizing green alga and rich in insoluble dietary fiber paramylon, can be enhanced by the co-consumption of vegetables that are rich in soluble dietary fiber.
30282906	1	57	theme	green	319:323	arg1	alga					325:328	a unicellular photosynthesizing green alga	287:328	a unicellular photosynthesizing green alga	287:328	We determined whether the benefits provided by the consumption of Euglena gracilis (Euglena), which is a unicellular photosynthesizing green alga and rich in insoluble dietary fiber paramylon, can be enhanced by the co-consumption of vegetables that are rich in soluble dietary fiber.
30282906	8	58	from	effect	1502:1507	arg1	accumulation					1551:1562	visceral fat accumulation	1538:1562	visceral fat accumulation	1538:1562	Therefore, co-consumption of Euglena with vegetables enhanced its anti-inflammatory effect and the inhibitory effect on visceral fat accumulation likely by modulating the composition of gut microbiota.
30282906	0	59	theme	Vegetables	44:53	arg1	Intake					13:18	Simultaneous Intake	0:18	Simultaneous Intake of Euglena Gracilis and Vegetables	0:53	Simultaneous Intake of Euglena Gracilis and Vegetables Synergistically Exerts an Anti-Inflammatory Effect and Attenuates Visceral Fat Accumulation by Affecting Gut Microbiota in Mice.
30282906	5	60	theme	acid	1111:1114	arg1	production					1116:1125	short-chain fatty acid production	1093:1125	short-chain fatty acid production	1093:1125	Notably, we observed an increase in the fraction of beneficial bacteria producing short-chain fatty acids, a reduction in harmful bacteria that cause inflammation and an increase in short-chain fatty acid production.
30282906	4	61	theme	Euglena	784:790	arg1	intake					792:797	Euglena intake	784:797	Euglena intake	784:797	Euglena intake significantly decreased serum markers of inflammation and co-consumption of vegetables enhanced this reduction.
30282906	8	62	theme	inhibitory	1517:1526	arg1	effect					1528:1533	the inhibitory effect	1513:1533	the inhibitory effect on visceral fat accumulation	1513:1562	Therefore, co-consumption of Euglena with vegetables enhanced its anti-inflammatory effect and the inhibitory effect on visceral fat accumulation likely by modulating the composition of gut microbiota.
30282906	7	63	theme	fatty	1316:1320	arg1	synthesis					1327:1335	fatty acid synthesis	1316:1335	fatty acid synthesis	1316:1335	Subsequent analyses showed that co-consumption of Euglena with vegetables reduced adipocyte area, suppressed the expression of genes related to fatty acid synthesis and increased the expression of genes related to adipocyte growth and lipolysis.
30282906	7	64	theme	Euglena	1222:1228	arg1	co-consumption					1204:1217	co-consumption	1204:1217	co-consumption of Euglena with vegetables	1204:1244	Subsequent analyses showed that co-consumption of Euglena with vegetables reduced adipocyte area, suppressed the expression of genes related to fatty acid synthesis and increased the expression of genes related to adipocyte growth and lipolysis.
30282906	4	65	theme	vegetables	875:884	arg1	co-consumption					857:870	co-consumption	857:870	co-consumption of vegetables	857:884	Euglena intake significantly decreased serum markers of inflammation and co-consumption of vegetables enhanced this reduction.
30282906	8	66	theme	fat	1547:1549	arg1	accumulation					1551:1562	visceral fat accumulation	1538:1562	visceral fat accumulation	1538:1562	Therefore, co-consumption of Euglena with vegetables enhanced its anti-inflammatory effect and the inhibitory effect on visceral fat accumulation likely by modulating the composition of gut microbiota.
30282906	2	67	theme	%	640:640	arg1	Euglena					642:648	1.0% Euglena	637:648	1.0% Euglena	637:648	Nine-week-old male C57BL/6J mice were divided into four groups: group 1 received normal diet, whereas groups 2, 3 and 4 received normal diet containing 0.3% paramylon, 1.0% Euglena, or 1.0% Euglena plus 0.3% vegetables (barley leaf, kale and ashitaba), respectively.
30282906	6	68	theme	fat	1137:1139	arg1	accumulation					1141:1152	Visceral fat accumulation	1128:1152	Visceral fat accumulation	1128:1152	Visceral fat accumulation was also reduced.
30282906	2	69	theme	normal	550:555	arg1	diet					557:560	normal diet	550:560	normal diet	550:560	Nine-week-old male C57BL/6J mice were divided into four groups: group 1 received normal diet, whereas groups 2, 3 and 4 received normal diet containing 0.3% paramylon, 1.0% Euglena, or 1.0% Euglena plus 0.3% vegetables (barley leaf, kale and ashitaba), respectively.
30282906	1	70	theme	vegetables	418:427	arg1	co-consumption					400:413	the co-consumption	396:413	the co-consumption of vegetables that are rich in soluble dietary fiber	396:466	We determined whether the benefits provided by the consumption of Euglena gracilis (Euglena), which is a unicellular photosynthesizing green alga and rich in insoluble dietary fiber paramylon, can be enhanced by the co-consumption of vegetables that are rich in soluble dietary fiber.
30282906	6	71	theme	Visceral	1128:1135	arg1	accumulation					1141:1152	Visceral fat accumulation	1128:1152	Visceral fat accumulation	1128:1152	Visceral fat accumulation was also reduced.
30282906	2	72	theme	0.3	672:674	arg1	%					675:675	%	675:675	%	675:675	Nine-week-old male C57BL/6J mice were divided into four groups: group 1 received normal diet, whereas groups 2, 3 and 4 received normal diet containing 0.3% paramylon, 1.0% Euglena, or 1.0% Euglena plus 0.3% vegetables (barley leaf, kale and ashitaba), respectively.
30282906	1	73	from	fiber	462:466	arg1	rich					438:441	rich	438:441	rich	438:441	We determined whether the benefits provided by the consumption of Euglena gracilis (Euglena), which is a unicellular photosynthesizing green alga and rich in insoluble dietary fiber paramylon, can be enhanced by the co-consumption of vegetables that are rich in soluble dietary fiber.
30282906	3	74	theme	ad	750:751	arg1	libitum					753:759	ad libitum	750:759	ad libitum	750:759	Mice were fed ad libitum until 18 weeks of age.
30282906	5	75	theme	bacteria	974:981	arg1	fraction					951:958	the fraction	947:958	the fraction of beneficial bacteria producing short-chain fatty acids	947:1015	Notably, we observed an increase in the fraction of beneficial bacteria producing short-chain fatty acids, a reduction in harmful bacteria that cause inflammation and an increase in short-chain fatty acid production.
30282906	8	76	theme	anti-inflammatory	1484:1500	arg1	effect					1502:1507	its anti-inflammatory effect	1480:1507	its anti-inflammatory effect	1480:1507	Therefore, co-consumption of Euglena with vegetables enhanced its anti-inflammatory effect and the inhibitory effect on visceral fat accumulation likely by modulating the composition of gut microbiota.
30282906	8	77	with	co-consumption	1429:1442	arg1	vegetables					1460:1469	vegetables	1460:1469	vegetables	1460:1469	Therefore, co-consumption of Euglena with vegetables enhanced its anti-inflammatory effect and the inhibitory effect on visceral fat accumulation likely by modulating the composition of gut microbiota.
30282906	1	78	theme	Euglena	250:256	arg1	Euglena					268:274	Euglena	268:274	Euglena	268:274	We determined whether the benefits provided by the consumption of Euglena gracilis (Euglena), which is a unicellular photosynthesizing green alga and rich in insoluble dietary fiber paramylon, can be enhanced by the co-consumption of vegetables that are rich in soluble dietary fiber.
30282906	1	78	theme	Euglena	250:256	arg1	gracilis					258:265	Euglena gracilis	250:265	Euglena gracilis (Euglena)	250:275	We determined whether the benefits provided by the consumption of Euglena gracilis (Euglena), which is a unicellular photosynthesizing green alga and rich in insoluble dietary fiber paramylon, can be enhanced by the co-consumption of vegetables that are rich in soluble dietary fiber.
30282906	1	78	theme	Euglena	250:256	arg1	alga					325:328	a unicellular photosynthesizing green alga	287:328	a unicellular photosynthesizing green alga	287:328	We determined whether the benefits provided by the consumption of Euglena gracilis (Euglena), which is a unicellular photosynthesizing green alga and rich in insoluble dietary fiber paramylon, can be enhanced by the co-consumption of vegetables that are rich in soluble dietary fiber.
30282906	5	79	theme	harmful	1033:1039	arg1	bacteria					1041:1048	harmful bacteria	1033:1048	harmful bacteria that cause inflammation	1033:1072	Notably, we observed an increase in the fraction of beneficial bacteria producing short-chain fatty acids, a reduction in harmful bacteria that cause inflammation and an increase in short-chain fatty acid production.
30282906	3	80	theme	age	779:781	arg1	weeks					770:774	18 weeks	767:774	18 weeks of age	767:781	Mice were fed ad libitum until 18 weeks of age.
30282906	1	81	theme	gracilis	258:265	arg1	consumption					235:245	the consumption	231:245	the consumption of Euglena gracilis (Euglena), which is a unicellular photosynthesizing green alga and rich in insoluble dietary fiber paramylon,	231:375	We determined whether the benefits provided by the consumption of Euglena gracilis (Euglena), which is a unicellular photosynthesizing green alga and rich in insoluble dietary fiber paramylon, can be enhanced by the co-consumption of vegetables that are rich in soluble dietary fiber.
31086081	4	0	gly	homogeneity	617:627	arg1	hydrogels					645:653	the prepared hydrogels	632:653	the prepared hydrogels	632:653	The homogeneity of the prepared hydrogels as revealed by the SEM show an excellent distribution of the oxidized polysaccharides inside the PVA matrix.
31086081	2	1	theme	vinyl	328:332	arg1	poly					323:326	poly	323:326	poly(vinyl alcohol) (PVA)	323:347	Thereafter, the composite hydrogels of poly(vinyl alcohol) (PVA) and different content of the oxidized polysaccharides were prepared by the freezing/thawing method.
31086081	2	1	theme	vinyl	328:332	arg1	alcohol					334:340	vinyl alcohol	328:340	vinyl alcohol	328:340	Thereafter, the composite hydrogels of poly(vinyl alcohol) (PVA) and different content of the oxidized polysaccharides were prepared by the freezing/thawing method.
31086081	5	2	theme	large	883:887	arg1	deformation					889:899	a large deformation	881:899	a large deformation	881:899	The samples exhibit self-healing features, since they quickly recover the initial structure after being subjected to a large deformation.
31086081	8	3	theme	physical	1255:1262	arg1	network					1264:1270	the physical network	1251:1270	the physical network	1251:1270	The synergism between the two constituents, PVA and oxidized polysaccharides, into the physical network, propose these hydrogels for many other biomedical applications.
31086081	0	4	theme	Dressing	81:88	arg1	Applications					90:101	Wound Dressing Applications	75:101	Wound Dressing Applications	75:101	Physical Hydrogels of Oxidized Polysaccharides and Poly(Vinyl Alcohol) for Wound Dressing Applications.
31086081	4	5	theme	polysaccharides	725:739	arg1	distribution					696:707	an excellent distribution	683:707	an excellent distribution of the oxidized polysaccharides inside the PVA matrix	683:761	The homogeneity of the prepared hydrogels as revealed by the SEM show an excellent distribution of the oxidized polysaccharides inside the PVA matrix.
31086081	0	6	theme	Wound	75:79	arg1	Applications					90:101	Wound Dressing Applications	75:101	Wound Dressing Applications	75:101	Physical Hydrogels of Oxidized Polysaccharides and Poly(Vinyl Alcohol) for Wound Dressing Applications.
31086081	4	7	theme	oxidized	716:723	arg1	polysaccharides					725:739	the oxidized polysaccharides	712:739	the oxidized polysaccharides	712:739	The homogeneity of the prepared hydrogels as revealed by the SEM show an excellent distribution of the oxidized polysaccharides inside the PVA matrix.
31086081	7	8	theme	imine-type	1126:1135	arg1	bonds					1137:1141	imine-type bonds	1126:1141	imine-type bonds	1126:1141	The samples are able to load L-arginine both by physical phenomena, such as diffusion, and also by chemical phenomena, when imine-type bonds are likely to be formed.
31086081	8	9	theme	oxidized	1220:1227	arg1	polysaccharides					1229:1243	oxidized polysaccharides	1220:1243	oxidized polysaccharides	1220:1243	The synergism between the two constituents, PVA and oxidized polysaccharides, into the physical network, propose these hydrogels for many other biomedical applications.
31086081	8	9	theme	oxidized	1220:1227	arg1	constituents					1198:1209	the two constituents	1190:1209	the two constituents	1190:1209	The synergism between the two constituents, PVA and oxidized polysaccharides, into the physical network, propose these hydrogels for many other biomedical applications.
31086081	7	10	theme	physical	1050:1057	arg1	phenomena					1059:1067	physical phenomena	1050:1067	physical phenomena	1050:1067	The samples are able to load L-arginine both by physical phenomena, such as diffusion, and also by chemical phenomena, when imine-type bonds are likely to be formed.
31086081	7	10	theme	physical	1050:1057	arg1	diffusion					1078:1086	diffusion	1078:1086	diffusion	1078:1086	The samples are able to load L-arginine both by physical phenomena, such as diffusion, and also by chemical phenomena, when imine-type bonds are likely to be formed.
31086081	3	11	dep	Fourier	453:459	arg1	transform					461:469	transform	461:469	transform infrared spectroscopy (FTIR)	461:498	The Fourier transform infrared spectroscopy (FTIR) has been used to discuss the degree of interaction between the hydrogels constituents into the physical network.
31086081	4	12	theme	prepared	636:643	arg1	hydrogels					645:653	the prepared hydrogels	632:653	the prepared hydrogels	632:653	The homogeneity of the prepared hydrogels as revealed by the SEM show an excellent distribution of the oxidized polysaccharides inside the PVA matrix.
31086081	1	13	theme	water	146:150	arg1	pullulan					160:167	water soluble pullulan	146:167	water soluble pullulan	146:167	Two natural polymers, i.e., cellulose and water soluble pullulan, have been selectively oxidized employing the TEMPO-mediated protocol, to allow the introduction of C6-OOH groups.
31086081	3	14	dep	transform	461:469	arg1	infrared					471:478	infrared	471:478	transform infrared spectroscopy (FTIR)	461:498	The Fourier transform infrared spectroscopy (FTIR) has been used to discuss the degree of interaction between the hydrogels constituents into the physical network.
31086081	1	15	theme	soluble	152:158	arg1	pullulan					160:167	water soluble pullulan	146:167	water soluble pullulan	146:167	Two natural polymers, i.e., cellulose and water soluble pullulan, have been selectively oxidized employing the TEMPO-mediated protocol, to allow the introduction of C6-OOH groups.
31086081	1	16	dep	cellulose	132:140	arg1	i.e.					126:129	i.e.	126:129	i.e.	126:129	Two natural polymers, i.e., cellulose and water soluble pullulan, have been selectively oxidized employing the TEMPO-mediated protocol, to allow the introduction of C6-OOH groups.
31086081	3	17	used	used	509:512	arg2	Fourier					453:459	The Fourier	449:459	The Fourier transform infrared spectroscopy (FTIR)	449:498	The Fourier transform infrared spectroscopy (FTIR) has been used to discuss the degree of interaction between the hydrogels constituents into the physical network.
31086081	0	18	theme	Physical	0:7	arg1	Hydrogels					9:17	Physical Hydrogels	0:17	Physical Hydrogels of Oxidized Polysaccharides and Poly(Vinyl Alcohol) for Wound Dressing Applications.	0:102	Physical Hydrogels of Oxidized Polysaccharides and Poly(Vinyl Alcohol) for Wound Dressing Applications.
31086081	6	19	theme	selected	943:950	arg1	all					963:965	all	963:965	all	963:965	The cell viability was performed for the selected hydrogels, all of them showing promising results.
31086081	6	19	theme	selected	943:950	arg1	hydrogels					952:960	the selected hydrogels	939:960	the selected hydrogels	939:960	The cell viability was performed for the selected hydrogels, all of them showing promising results.
31086081	6	19	theme	selected	943:950	arg1	them					970:973	them	970:973	them	970:973	The cell viability was performed for the selected hydrogels, all of them showing promising results.
31086081	4	20	theme	excellent	686:694	arg1	distribution					696:707	an excellent distribution	683:707	an excellent distribution of the oxidized polysaccharides inside the PVA matrix	683:761	The homogeneity of the prepared hydrogels as revealed by the SEM show an excellent distribution of the oxidized polysaccharides inside the PVA matrix.
31086081	2	21	theme	polysaccharides	387:401	arg1	content					363:369	different content	353:369	different content of the oxidized polysaccharides	353:401	Thereafter, the composite hydrogels of poly(vinyl alcohol) (PVA) and different content of the oxidized polysaccharides were prepared by the freezing/thawing method.
31086081	2	21	theme	polysaccharides	387:401	arg1	poly					323:326	poly	323:326	poly(vinyl alcohol) (PVA)	323:347	Thereafter, the composite hydrogels of poly(vinyl alcohol) (PVA) and different content of the oxidized polysaccharides were prepared by the freezing/thawing method.
31086081	2	21	theme	polysaccharides	387:401	arg1	alcohol					334:340	vinyl alcohol	328:340	vinyl alcohol	328:340	Thereafter, the composite hydrogels of poly(vinyl alcohol) (PVA) and different content of the oxidized polysaccharides were prepared by the freezing/thawing method.
31086081	2	21	theme	polysaccharides	387:401	arg1	PVA					344:346	PVA	344:346	PVA	344:346	Thereafter, the composite hydrogels of poly(vinyl alcohol) (PVA) and different content of the oxidized polysaccharides were prepared by the freezing/thawing method.
31086081	7	22	theme	load	1026:1029	arg1	L-arginine					1031:1040	load L-arginine	1026:1040	load L-arginine	1026:1040	The samples are able to load L-arginine both by physical phenomena, such as diffusion, and also by chemical phenomena, when imine-type bonds are likely to be formed.
31086081	8	23	theme	biomedical	1312:1321	arg1	applications					1323:1334	many other biomedical applications	1301:1334	many other biomedical applications	1301:1334	The synergism between the two constituents, PVA and oxidized polysaccharides, into the physical network, propose these hydrogels for many other biomedical applications.
31086081	2	24	theme	oxidized	378:385	arg1	polysaccharides					387:401	the oxidized polysaccharides	374:401	the oxidized polysaccharides	374:401	Thereafter, the composite hydrogels of poly(vinyl alcohol) (PVA) and different content of the oxidized polysaccharides were prepared by the freezing/thawing method.
31086081	1	25	theme	C6-OOH	269:274	arg1	groups					276:281	C6-OOH groups	269:281	C6-OOH groups	269:281	Two natural polymers, i.e., cellulose and water soluble pullulan, have been selectively oxidized employing the TEMPO-mediated protocol, to allow the introduction of C6-OOH groups.
31086081	8	26	theme	other	1306:1310	arg1	applications					1323:1334	many other biomedical applications	1301:1334	many other biomedical applications	1301:1334	The synergism between the two constituents, PVA and oxidized polysaccharides, into the physical network, propose these hydrogels for many other biomedical applications.
31086081	5	27	theme	self-healing	784:795	arg1	features					797:804	self-healing features	784:804	self-healing features	784:804	The samples exhibit self-healing features, since they quickly recover the initial structure after being subjected to a large deformation.
31086081	1	28	theme	groups	276:281	arg1	introduction					253:264	the introduction	249:264	the introduction of C6-OOH groups	249:281	Two natural polymers, i.e., cellulose and water soluble pullulan, have been selectively oxidized employing the TEMPO-mediated protocol, to allow the introduction of C6-OOH groups.
31086081	2	29	theme	content	363:369	arg1	hydrogels					310:318	the composite hydrogels	296:318	the composite hydrogels of poly(vinyl alcohol) (PVA) and different content of the oxidized polysaccharides	296:401	Thereafter, the composite hydrogels of poly(vinyl alcohol) (PVA) and different content of the oxidized polysaccharides were prepared by the freezing/thawing method.
31086081	2	30	theme	freezing/thawing	424:439	arg1	method					441:446	the freezing/thawing method	420:446	the freezing/thawing method	420:446	Thereafter, the composite hydrogels of poly(vinyl alcohol) (PVA) and different content of the oxidized polysaccharides were prepared by the freezing/thawing method.
31086081	0	31	theme	Polysaccharides	31:45	arg1	Hydrogels					9:17	Physical Hydrogels	0:17	Physical Hydrogels of Oxidized Polysaccharides and Poly(Vinyl Alcohol) for Wound Dressing Applications.	0:102	Physical Hydrogels of Oxidized Polysaccharides and Poly(Vinyl Alcohol) for Wound Dressing Applications.
31086081	3	32	theme	physical	595:602	arg1	network					604:610	the physical network	591:610	the physical network	591:610	The Fourier transform infrared spectroscopy (FTIR) has been used to discuss the degree of interaction between the hydrogels constituents into the physical network.
31086081	0	33	theme	Oxidized	22:29	arg1	Polysaccharides					31:45	Oxidized Polysaccharides	22:45	Oxidized Polysaccharides	22:45	Physical Hydrogels of Oxidized Polysaccharides and Poly(Vinyl Alcohol) for Wound Dressing Applications.
31086081	2	34	theme	different	353:361	arg1	content					363:369	different content	353:369	different content of the oxidized polysaccharides	353:401	Thereafter, the composite hydrogels of poly(vinyl alcohol) (PVA) and different content of the oxidized polysaccharides were prepared by the freezing/thawing method.
31086081	5	35	theme	initial	838:844	arg1	structure					846:854	the initial structure	834:854	the initial structure	834:854	The samples exhibit self-healing features, since they quickly recover the initial structure after being subjected to a large deformation.
31086081	8	36	theme	many	1301:1304	arg1	applications					1323:1334	many other biomedical applications	1301:1334	many other biomedical applications	1301:1334	The synergism between the two constituents, PVA and oxidized polysaccharides, into the physical network, propose these hydrogels for many other biomedical applications.
31086081	2	37	theme	poly	323:326	arg1	hydrogels					310:318	the composite hydrogels	296:318	the composite hydrogels of poly(vinyl alcohol) (PVA) and different content of the oxidized polysaccharides	296:401	Thereafter, the composite hydrogels of poly(vinyl alcohol) (PVA) and different content of the oxidized polysaccharides were prepared by the freezing/thawing method.
31086081	0	38	theme	Poly	51:54	arg1	Hydrogels					9:17	Physical Hydrogels	0:17	Physical Hydrogels of Oxidized Polysaccharides and Poly(Vinyl Alcohol) for Wound Dressing Applications.	0:102	Physical Hydrogels of Oxidized Polysaccharides and Poly(Vinyl Alcohol) for Wound Dressing Applications.
31086081	6	39	theme	cell	906:909	arg1	viability					911:919	The cell viability	902:919	The cell viability	902:919	The cell viability was performed for the selected hydrogels, all of them showing promising results.
31086081	3	40	theme	interaction	539:549	arg1	degree					529:534	the degree	525:534	the degree of interaction between the hydrogels constituents into the physical network	525:610	The Fourier transform infrared spectroscopy (FTIR) has been used to discuss the degree of interaction between the hydrogels constituents into the physical network.
31086081	1	41	theme	natural	108:114	arg1	polymers					116:123	Two natural polymers	104:123	Two natural polymers	104:123	Two natural polymers, i.e., cellulose and water soluble pullulan, have been selectively oxidized employing the TEMPO-mediated protocol, to allow the introduction of C6-OOH groups.
31086081	7	42	theme	chemical	1101:1108	arg1	phenomena					1110:1118	chemical phenomena	1101:1118	chemical phenomena	1101:1118	The samples are able to load L-arginine both by physical phenomena, such as diffusion, and also by chemical phenomena, when imine-type bonds are likely to be formed.
31086081	4	43	theme	PVA	752:754	arg1	matrix					756:761	the PVA matrix	748:761	the PVA matrix	748:761	The homogeneity of the prepared hydrogels as revealed by the SEM show an excellent distribution of the oxidized polysaccharides inside the PVA matrix.
31086081	2	44	theme	composite	300:308	arg1	hydrogels					310:318	the composite hydrogels	296:318	the composite hydrogels of poly(vinyl alcohol) (PVA) and different content of the oxidized polysaccharides	296:401	Thereafter, the composite hydrogels of poly(vinyl alcohol) (PVA) and different content of the oxidized polysaccharides were prepared by the freezing/thawing method.
31086081	6	45	theme	promising	983:991	arg1	results					993:999	promising results	983:999	promising results	983:999	The cell viability was performed for the selected hydrogels, all of them showing promising results.
31086081	3	46	theme	hydrogels	563:571	arg1	constituents					573:584	the hydrogels constituents	559:584	the hydrogels constituents into the physical network	559:610	The Fourier transform infrared spectroscopy (FTIR) has been used to discuss the degree of interaction between the hydrogels constituents into the physical network.
31086081	1	47	theme	TEMPO-mediated	215:228	arg1	protocol					230:237	the TEMPO-mediated protocol	211:237	the TEMPO-mediated protocol	211:237	Two natural polymers, i.e., cellulose and water soluble pullulan, have been selectively oxidized employing the TEMPO-mediated protocol, to allow the introduction of C6-OOH groups.
31086081	0	48	theme	Vinyl	56:60	arg1	Poly					51:54	Poly	51:54	Poly(Vinyl Alcohol)	51:69	Physical Hydrogels of Oxidized Polysaccharides and Poly(Vinyl Alcohol) for Wound Dressing Applications.
31086081	0	48	theme	Vinyl	56:60	arg1	Alcohol					62:68	Vinyl Alcohol	56:68	Vinyl Alcohol	56:68	Physical Hydrogels of Oxidized Polysaccharides and Poly(Vinyl Alcohol) for Wound Dressing Applications.
31086081	4	49	dep	homogeneity	617:627	arg1	revealed					658:665	revealed	658:665	revealed by the SEM	658:676	The homogeneity of the prepared hydrogels as revealed by the SEM show an excellent distribution of the oxidized polysaccharides inside the PVA matrix.
31086081	4	50	theme	hydrogels	645:653	arg1	homogeneity					617:627	The homogeneity	613:627	The homogeneity of the prepared hydrogels as revealed by the SEM	613:676	The homogeneity of the prepared hydrogels as revealed by the SEM show an excellent distribution of the oxidized polysaccharides inside the PVA matrix.
29959009	4	0	theme	breaking	445:452	arg1	strengths					454:462	the water absorption, porosity and breaking strengths	410:462	the water absorption, porosity and breaking strengths of the hydrogel	410:478	With the increase of HA content, the water absorption, porosity and breaking strengths of the hydrogel increased, while hydrophilic of the hydrogel was enhanced extremely determined by contact angle decreasing.
29959009	5	1	theme	SF/HA	604:608	arg1	hydrogel					610:617	the SF/HA hydrogel	600:617	the SF/HA hydrogel with a ratio of 5:5	600:637	Especially, the SF/HA hydrogel with a ratio of 5:5 presented the highest water absorption and mechanical properties.
29959009	9	2	theme	SF/HA	1080:1084	arg1	hydrogels					1086:1094	the SF/HA hydrogels	1076:1094	the SF/HA hydrogels	1076:1094	In vivo, the SF/HA hydrogels presented good histocompatibility and promoted vascular-like tissue regeneration when were implanted subcutaneously of Sprague Dawley rats.
29959009	6	3	theme	content	881:887	arg1	increase					866:873	the increase	862:873	the increase of HA content	862:887	The molecule conformation of the composite hydrogel was mainly amorphous structure and contained the small number of β-sheets which gradually decreases with the increase of HA content.
29959009	6	4	theme	HA	878:879	arg1	content					881:887	HA content	878:887	HA content	878:887	The molecule conformation of the composite hydrogel was mainly amorphous structure and contained the small number of β-sheets which gradually decreases with the increase of HA content.
29959009	7	5	theme	release	925:931	arg1	ratio					933:937	accumulative release ratio	912:937	accumulative release ratio of the composite gel	912:958	In drug release test, accumulative release ratio of the composite gel was about 80% at day 40.
29959009	5	6	with	hydrogel	610:617	arg1	ratio					626:630	a ratio	624:630	a ratio of 5:5	624:637	Especially, the SF/HA hydrogel with a ratio of 5:5 presented the highest water absorption and mechanical properties.
29959009	6	7	theme	β-sheets	822:829	arg1	number					812:817	the small number	802:817	the small number of β-sheets which gradually decreases with the increase of HA content	802:887	The molecule conformation of the composite hydrogel was mainly amorphous structure and contained the small number of β-sheets which gradually decreases with the increase of HA content.
29959009	9	8	theme	good	1106:1109	arg1	histocompatibility					1111:1128	good histocompatibility	1106:1128	good histocompatibility	1106:1128	In vivo, the SF/HA hydrogels presented good histocompatibility and promoted vascular-like tissue regeneration when were implanted subcutaneously of Sprague Dawley rats.
29959009	5	9	theme	mechanical	682:691	arg1	properties					693:702	mechanical properties	682:702	mechanical properties	682:702	Especially, the SF/HA hydrogel with a ratio of 5:5 presented the highest water absorption and mechanical properties.
29959009	6	10	theme	amorphous	768:776	arg1	conformation					718:729	The molecule conformation	705:729	The molecule conformation of the composite hydrogel	705:755	The molecule conformation of the composite hydrogel was mainly amorphous structure and contained the small number of β-sheets which gradually decreases with the increase of HA content.
29959009	6	10	theme	amorphous	768:776	arg1	structure					778:786	amorphous structure	768:786	amorphous structure	768:786	The molecule conformation of the composite hydrogel was mainly amorphous structure and contained the small number of β-sheets which gradually decreases with the increase of HA content.
29959009	2	11	theme	hyaluronic	263:272	arg1	HA					280:281	HA	280:281	HA	280:281	In this study, we established a rapid hydrogel to imitate the nature extracellular matrix via silk fibroin (SF) and hyaluronic acid (HA) blend hydrogel.
29959009	2	11	theme	hyaluronic	263:272	arg1	acid					274:277	hyaluronic acid	263:277	hyaluronic acid (HA)	263:282	In this study, we established a rapid hydrogel to imitate the nature extracellular matrix via silk fibroin (SF) and hyaluronic acid (HA) blend hydrogel.
29959009	4	12	theme	absorption	420:429	arg1	strengths					454:462	the water absorption, porosity and breaking strengths	410:462	the water absorption, porosity and breaking strengths of the hydrogel	410:478	With the increase of HA content, the water absorption, porosity and breaking strengths of the hydrogel increased, while hydrophilic of the hydrogel was enhanced extremely determined by contact angle decreasing.
29959009	7	13	theme	accumulative	912:923	arg1	ratio					933:937	accumulative release ratio	912:937	accumulative release ratio of the composite gel	912:958	In drug release test, accumulative release ratio of the composite gel was about 80% at day 40.
29959009	2	14	dep	fibroin	246:252	arg1	hydrogel					290:297	blend hydrogel	284:297	blend hydrogel	284:297	In this study, we established a rapid hydrogel to imitate the nature extracellular matrix via silk fibroin (SF) and hyaluronic acid (HA) blend hydrogel.
29959009	7	15	theme	release	898:904	arg1	test					906:909	drug release test	893:909	drug release test	893:909	In drug release test, accumulative release ratio of the composite gel was about 80% at day 40.
29959009	3	16	theme	formation	349:357	arg1	time					359:362	its formation time	345:362	its formation time	345:362	SF/HA hydrogel was prepared effectively, and its formation time was shorted.
29959009	4	17	theme	porosity	432:439	arg1	strengths					454:462	the water absorption, porosity and breaking strengths	410:462	the water absorption, porosity and breaking strengths of the hydrogel	410:478	With the increase of HA content, the water absorption, porosity and breaking strengths of the hydrogel increased, while hydrophilic of the hydrogel was enhanced extremely determined by contact angle decreasing.
29959009	6	18	theme	hydrogel	748:755	arg1	structure					778:786	amorphous structure	768:786	amorphous structure	768:786	The molecule conformation of the composite hydrogel was mainly amorphous structure and contained the small number of β-sheets which gradually decreases with the increase of HA content.
29959009	6	18	theme	hydrogel	748:755	arg1	conformation					718:729	The molecule conformation	705:729	The molecule conformation of the composite hydrogel	705:755	The molecule conformation of the composite hydrogel was mainly amorphous structure and contained the small number of β-sheets which gradually decreases with the increase of HA content.
29959009	2	19	theme	blend	284:288	arg1	hydrogel					290:297	blend hydrogel	284:297	blend hydrogel	284:297	In this study, we established a rapid hydrogel to imitate the nature extracellular matrix via silk fibroin (SF) and hyaluronic acid (HA) blend hydrogel.
29959009	5	20	theme	water	661:665	arg1	absorption					667:676	water absorption	661:676	water absorption	661:676	Especially, the SF/HA hydrogel with a ratio of 5:5 presented the highest water absorption and mechanical properties.
29959009	0	21	theme	Facile	0:5	arg1	preparation					7:17	Facile preparation	0:17	Facile preparation of bioactive silk	0:35	Facile preparation of bioactive silk fibroin/hyaluronic acid hydrogels.
29959009	8	22	theme	mass	993:996	arg1	loss					998:1001	the mass loss	989:1001	the mass loss of the hydrogel	989:1017	And the mass loss of the hydrogel reached approximately 78% in vitro degradation.
29959009	6	23	theme	composite	738:746	arg1	hydrogel					748:755	the composite hydrogel	734:755	the composite hydrogel	734:755	The molecule conformation of the composite hydrogel was mainly amorphous structure and contained the small number of β-sheets which gradually decreases with the increase of HA content.
29959009	1	24	theme	primary	105:111	arg1	polysaccharides					85:99	polysaccharides	85:99	polysaccharides	85:99	Proteins and polysaccharides are primary components in mammal soft tissue.
29959009	1	24	theme	primary	105:111	arg1	components					113:122	primary components	105:122	primary components in mammal soft tissue	105:144	Proteins and polysaccharides are primary components in mammal soft tissue.
29959009	1	24	theme	primary	105:111	arg1	Proteins					72:79	Proteins	72:79	Proteins	72:79	Proteins and polysaccharides are primary components in mammal soft tissue.
29959009	0	25	theme	bioactive	22:30	arg1	silk					32:35	bioactive silk	22:35	bioactive silk	22:35	Facile preparation of bioactive silk fibroin/hyaluronic acid hydrogels.
29959009	9	26	theme	vascular-like	1143:1155	arg1	regeneration					1164:1175	vascular-like tissue regeneration	1143:1175	vascular-like tissue regeneration	1143:1175	In vivo, the SF/HA hydrogels presented good histocompatibility and promoted vascular-like tissue regeneration when were implanted subcutaneously of Sprague Dawley rats.
29959009	7	27	theme	drug	893:896	arg1	test					906:909	drug release test	893:909	drug release test	893:909	In drug release test, accumulative release ratio of the composite gel was about 80% at day 40.
29959009	10	28	theme	new	1258:1260	arg1	approach					1262:1269	a new approach	1256:1269	a new approach to fabricate silk-based biomaterials for soft tissue regeneration	1256:1335	This study provides a new approach to fabricate silk-based biomaterials for soft tissue regeneration.
29959009	10	29	theme	tissue	1317:1322	arg1	regeneration					1324:1335	soft tissue regeneration	1312:1335	soft tissue regeneration	1312:1335	This study provides a new approach to fabricate silk-based biomaterials for soft tissue regeneration.
29959009	4	30	theme	hydrogel	516:523	arg1	hydrophilic					497:507	hydrophilic	497:507	hydrophilic	497:507	With the increase of HA content, the water absorption, porosity and breaking strengths of the hydrogel increased, while hydrophilic of the hydrogel was enhanced extremely determined by contact angle decreasing.
29959009	5	31	theme	highest	653:659	arg1	absorption					667:676	water absorption	661:676	water absorption	661:676	Especially, the SF/HA hydrogel with a ratio of 5:5 presented the highest water absorption and mechanical properties.
29959009	2	32	theme	rapid	179:183	arg1	hydrogel					185:192	a rapid hydrogel	177:192	a rapid hydrogel to imitate the nature extracellular matrix via silk fibroin (SF) and hyaluronic acid (HA) blend hydrogel	177:297	In this study, we established a rapid hydrogel to imitate the nature extracellular matrix via silk fibroin (SF) and hyaluronic acid (HA) blend hydrogel.
29959009	4	33	dep	enhanced	529:536	arg1	determined					548:557	determined	548:557	was enhanced extremely determined by contact angle decreasing	525:585	With the increase of HA content, the water absorption, porosity and breaking strengths of the hydrogel increased, while hydrophilic of the hydrogel was enhanced extremely determined by contact angle decreasing.
29959009	4	34	theme	content	401:407	arg1	increase					386:393	the increase	382:393	the increase of HA content	382:407	With the increase of HA content, the water absorption, porosity and breaking strengths of the hydrogel increased, while hydrophilic of the hydrogel was enhanced extremely determined by contact angle decreasing.
29959009	0	35	theme	silk	32:35	arg1	preparation					7:17	Facile preparation	0:17	Facile preparation of bioactive silk	0:35	Facile preparation of bioactive silk fibroin/hyaluronic acid hydrogels.
29959009	8	36	theme	hydrogel	1010:1017	arg1	loss					998:1001	the mass loss	989:1001	the mass loss of the hydrogel	989:1017	And the mass loss of the hydrogel reached approximately 78% in vitro degradation.
29959009	4	37	theme	water	414:418	arg1	absorption					420:429	water absorption	414:429	water absorption	414:429	With the increase of HA content, the water absorption, porosity and breaking strengths of the hydrogel increased, while hydrophilic of the hydrogel was enhanced extremely determined by contact angle decreasing.
29959009	7	38	from	%	972:972	arg1	test					906:909	drug release test	893:909	drug release test	893:909	In drug release test, accumulative release ratio of the composite gel was about 80% at day 40.
29959009	4	39	theme	hydrogel	471:478	arg1	strengths					454:462	the water absorption, porosity and breaking strengths	410:462	the water absorption, porosity and breaking strengths of the hydrogel	410:478	With the increase of HA content, the water absorption, porosity and breaking strengths of the hydrogel increased, while hydrophilic of the hydrogel was enhanced extremely determined by contact angle decreasing.
29959009	2	40	theme	extracellular	216:228	arg1	matrix					230:235	the nature extracellular matrix	205:235	the nature extracellular matrix	205:235	In this study, we established a rapid hydrogel to imitate the nature extracellular matrix via silk fibroin (SF) and hyaluronic acid (HA) blend hydrogel.
29959009	10	41	theme	silk-based	1284:1293	arg1	biomaterials					1295:1306	silk-based biomaterials	1284:1306	silk-based biomaterials	1284:1306	This study provides a new approach to fabricate silk-based biomaterials for soft tissue regeneration.
29959009	1	42	theme	mammal	127:132	arg1	tissue					139:144	mammal soft tissue	127:144	mammal soft tissue	127:144	Proteins and polysaccharides are primary components in mammal soft tissue.
29959009	0	43	theme	acid	56:59	arg1	hydrogels					61:69	acid hydrogels	56:69	acid hydrogels	56:69	Facile preparation of bioactive silk fibroin/hyaluronic acid hydrogels.
29959009	2	44	theme	nature	209:214	arg1	matrix					230:235	the nature extracellular matrix	205:235	the nature extracellular matrix	205:235	In this study, we established a rapid hydrogel to imitate the nature extracellular matrix via silk fibroin (SF) and hyaluronic acid (HA) blend hydrogel.
29959009	6	45	theme	molecule	709:716	arg1	structure					778:786	amorphous structure	768:786	amorphous structure	768:786	The molecule conformation of the composite hydrogel was mainly amorphous structure and contained the small number of β-sheets which gradually decreases with the increase of HA content.
29959009	6	45	theme	molecule	709:716	arg1	conformation					718:729	The molecule conformation	705:729	The molecule conformation of the composite hydrogel	705:755	The molecule conformation of the composite hydrogel was mainly amorphous structure and contained the small number of β-sheets which gradually decreases with the increase of HA content.
29959009	1	46	theme	soft	134:137	arg1	tissue					139:144	mammal soft tissue	127:144	mammal soft tissue	127:144	Proteins and polysaccharides are primary components in mammal soft tissue.
29959009	1	47	from	components	113:122	arg1	tissue					139:144	mammal soft tissue	127:144	mammal soft tissue	127:144	Proteins and polysaccharides are primary components in mammal soft tissue.
29959009	9	48	theme	tissue	1157:1162	arg1	regeneration					1164:1175	vascular-like tissue regeneration	1143:1175	vascular-like tissue regeneration	1143:1175	In vivo, the SF/HA hydrogels presented good histocompatibility and promoted vascular-like tissue regeneration when were implanted subcutaneously of Sprague Dawley rats.
29959009	5	49	theme	5:5	635:637	arg1	ratio					626:630	a ratio	624:630	a ratio of 5:5	624:637	Especially, the SF/HA hydrogel with a ratio of 5:5 presented the highest water absorption and mechanical properties.
29959009	6	50	theme	small	806:810	arg1	number					812:817	the small number	802:817	the small number of β-sheets which gradually decreases with the increase of HA content	802:887	The molecule conformation of the composite hydrogel was mainly amorphous structure and contained the small number of β-sheets which gradually decreases with the increase of HA content.
29959009	4	51	theme	contact	562:568	arg1	angle					570:574	contact angle decreasing	562:585	contact angle decreasing	562:585	With the increase of HA content, the water absorption, porosity and breaking strengths of the hydrogel increased, while hydrophilic of the hydrogel was enhanced extremely determined by contact angle decreasing.
29959009	4	52	theme	HA	398:399	arg1	content					401:407	HA content	398:407	HA content	398:407	With the increase of HA content, the water absorption, porosity and breaking strengths of the hydrogel increased, while hydrophilic of the hydrogel was enhanced extremely determined by contact angle decreasing.
29959009	7	53	theme	composite	946:954	arg1	gel					956:958	the composite gel	942:958	the composite gel	942:958	In drug release test, accumulative release ratio of the composite gel was about 80% at day 40.
29959009	7	54	from	day	977:979	arg1	%					972:972	about 80%	964:972	about 80%	964:972	In drug release test, accumulative release ratio of the composite gel was about 80% at day 40.
29959009	3	55	theme	SF/HA	300:304	arg1	hydrogel					306:313	SF/HA hydrogel	300:313	SF/HA hydrogel	300:313	SF/HA hydrogel was prepared effectively, and its formation time was shorted.
29959009	9	56	theme	Dawley	1223:1228	arg1	rats					1230:1233	Sprague Dawley rats	1215:1233	Sprague Dawley rats	1215:1233	In vivo, the SF/HA hydrogels presented good histocompatibility and promoted vascular-like tissue regeneration when were implanted subcutaneously of Sprague Dawley rats.
29959009	7	57	theme	gel	956:958	arg1	ratio					933:937	accumulative release ratio	912:937	accumulative release ratio of the composite gel	912:958	In drug release test, accumulative release ratio of the composite gel was about 80% at day 40.
29959009	6	58	contain	contained	792:800	arg2	number					812:817	the small number	802:817	the small number of β-sheets which gradually decreases with the increase of HA content	802:887	The molecule conformation of the composite hydrogel was mainly amorphous structure and contained the small number of β-sheets which gradually decreases with the increase of HA content.
29959009	6	58	contain	contained	792:800	arg1	structure					778:786	amorphous structure	768:786	amorphous structure	768:786	The molecule conformation of the composite hydrogel was mainly amorphous structure and contained the small number of β-sheets which gradually decreases with the increase of HA content.
29959009	6	58	contain	contained	792:800	arg1	conformation					718:729	The molecule conformation	705:729	The molecule conformation of the composite hydrogel	705:755	The molecule conformation of the composite hydrogel was mainly amorphous structure and contained the small number of β-sheets which gradually decreases with the increase of HA content.
29959009	4	59	theme	decreasing	576:585	arg1	angle					570:574	contact angle decreasing	562:585	contact angle decreasing	562:585	With the increase of HA content, the water absorption, porosity and breaking strengths of the hydrogel increased, while hydrophilic of the hydrogel was enhanced extremely determined by contact angle decreasing.
29959009	8	60	theme	vitro	1048:1052	arg1	degradation					1054:1064	vitro degradation	1048:1064	vitro degradation	1048:1064	And the mass loss of the hydrogel reached approximately 78% in vitro degradation.
29959009	2	61	theme	silk	241:244	arg1	fibroin					246:252	silk fibroin	241:252	silk fibroin (SF)	241:257	In this study, we established a rapid hydrogel to imitate the nature extracellular matrix via silk fibroin (SF) and hyaluronic acid (HA) blend hydrogel.
29959009	2	61	theme	silk	241:244	arg1	SF					255:256	SF	255:256	SF	255:256	In this study, we established a rapid hydrogel to imitate the nature extracellular matrix via silk fibroin (SF) and hyaluronic acid (HA) blend hydrogel.
29959009	10	62	theme	soft	1312:1315	arg1	regeneration					1324:1335	soft tissue regeneration	1312:1335	soft tissue regeneration	1312:1335	This study provides a new approach to fabricate silk-based biomaterials for soft tissue regeneration.
35541777	0	0	theme	Ca2+	62:65	arg1	mechanism					38:46	the organic fouling mechanism	18:46	the organic fouling mechanism of an in situ Ca2+	18:65	New insights into the organic fouling mechanism of an in situ Ca2+ modified thin film composite forward osmosis membrane.
35541777	1	1	dep	in	239:240	arg1	situ					242:245	situ	242:245	situ	242:245	In this study, the effect of organic substances on the fouling behavior of a thin film composite (TFC) membrane with in situ Ca2+ addition (TFC-Ca membrane) was evaluated.
35541777	1	2	from	effect	141:146	arg1	behavior					185:192	the fouling behavior	173:192	the fouling behavior of a thin film composite (TFC) membrane with in situ Ca2+ addition (TFC-Ca membrane)	173:277	In this study, the effect of organic substances on the fouling behavior of a thin film composite (TFC) membrane with in situ Ca2+ addition (TFC-Ca membrane) was evaluated.
35541777	1	3	theme	film	204:207	arg1	membrane					225:232	a thin film composite (TFC) membrane	197:232	a thin film composite (TFC) membrane with in situ Ca2+ addition (TFC-Ca membrane)	197:277	In this study, the effect of organic substances on the fouling behavior of a thin film composite (TFC) membrane with in situ Ca2+ addition (TFC-Ca membrane) was evaluated.
35541777	8	4	theme	membranes	1436:1444	arg1	development					1405:1415	the development	1401:1415	the development of antifouling TFC membranes	1401:1444	The antifouling mechanism of Ca2+ pre-binding carboxyl groups in the TFC-Ca FO membrane was demonstrated, which provides new insights into the development of antifouling TFC membranes in the future.
35541777	4	5	theme	TFC-Ca	777:782	arg1	membrane					784:791	the fouled TFC-Ca membrane	766:791	the fouled TFC-Ca membrane	766:791	SEM-EDX, ICP-OES and TOC analysis were applied to characterize the fouled TFC-Ca membrane.
35541777	5	6	theme	interfacial	821:831	arg1	energies					838:845	the interfacial free energies	817:845	the interfacial free energies obtained from advanced contact angle measurements	817:895	Results suggested that the interfacial free energies obtained from advanced contact angle measurements were correlated strongly with the rates of membrane fouling.
35541777	1	7	theme	composite	209:217	arg1	membrane					225:232	a thin film composite (TFC) membrane	197:232	a thin film composite (TFC) membrane with in situ Ca2+ addition (TFC-Ca membrane)	197:277	In this study, the effect of organic substances on the fouling behavior of a thin film composite (TFC) membrane with in situ Ca2+ addition (TFC-Ca membrane) was evaluated.
35541777	0	8	theme	thin	76:79	arg1	film					81:84	thin film	76:84	thin film composite forward osmosis membrane	76:119	New insights into the organic fouling mechanism of an in situ Ca2+ modified thin film composite forward osmosis membrane.
35541777	8	9	theme	TFC	1432:1434	arg1	membranes					1436:1444	antifouling TFC membranes	1420:1444	antifouling TFC membranes	1420:1444	The antifouling mechanism of Ca2+ pre-binding carboxyl groups in the TFC-Ca FO membrane was demonstrated, which provides new insights into the development of antifouling TFC membranes in the future.
35541777	2	10	theme	fouling	545:551	arg1	process					553:559	the membrane fouling process	532:559	the membrane fouling process	532:559	Bovine serum albumin (BSA), humic acid (HA) and sodium alginate (SA) were used as surrogate foulants for protein, natural organic substances and polysaccharides, respectively, thus enabling the analysis of foulant-membrane interaction in the membrane fouling process.
35541777	8	11	theme	pre-binding	1296:1306	arg1	groups					1317:1322	Ca2+ pre-binding carboxyl groups	1291:1322	Ca2+ pre-binding carboxyl groups in the TFC-Ca FO membrane	1291:1348	The antifouling mechanism of Ca2+ pre-binding carboxyl groups in the TFC-Ca FO membrane was demonstrated, which provides new insights into the development of antifouling TFC membranes in the future.
35541777	6	12	theme	foulant-membrane	1072:1087	arg1	interaction					1089:1099	foulant-membrane interaction	1072:1099	foulant-membrane interaction	1072:1099	In situ Ca2+ addition in the TFC membrane resulted in the decrease of the interfacial adhesion free energy (i.e., foulant-membrane interaction) and thus the mitigation of membrane fouling.
35541777	6	12	theme	foulant-membrane	1072:1087	arg1	energy					1058:1063	the interfacial adhesion free energy	1028:1063	the interfacial adhesion free energy (i.e., foulant-membrane interaction)	1028:1100	In situ Ca2+ addition in the TFC membrane resulted in the decrease of the interfacial adhesion free energy (i.e., foulant-membrane interaction) and thus the mitigation of membrane fouling.
35541777	2	13	theme	membrane	536:543	arg1	process					553:559	the membrane fouling process	532:559	the membrane fouling process	532:559	Bovine serum albumin (BSA), humic acid (HA) and sodium alginate (SA) were used as surrogate foulants for protein, natural organic substances and polysaccharides, respectively, thus enabling the analysis of foulant-membrane interaction in the membrane fouling process.
35541777	1	14	theme	TFC	220:222	arg1	membrane					225:232	a thin film composite (TFC) membrane	197:232	a thin film composite (TFC) membrane with in situ Ca2+ addition (TFC-Ca membrane)	197:277	In this study, the effect of organic substances on the fouling behavior of a thin film composite (TFC) membrane with in situ Ca2+ addition (TFC-Ca membrane) was evaluated.
35541777	0	15	theme	composite	86:94	arg1	membrane					112:119	thin film composite forward osmosis membrane	76:119	thin film composite forward osmosis membrane	76:119	New insights into the organic fouling mechanism of an in situ Ca2+ modified thin film composite forward osmosis membrane.
35541777	6	16	theme	energy	1058:1063	arg1	decrease					1016:1023	the decrease	1012:1023	the decrease of the interfacial adhesion free energy (i.e., foulant-membrane interaction)	1012:1100	In situ Ca2+ addition in the TFC membrane resulted in the decrease of the interfacial adhesion free energy (i.e., foulant-membrane interaction) and thus the mitigation of membrane fouling.
35541777	7	17	theme	physical	1243:1250	arg1	cleaning					1252:1259	simple physical cleaning	1236:1259	simple physical cleaning	1236:1259	The permeate flux of TFC-Ca FO membrane after organic fouling could be fully restored by simple physical cleaning.
35541777	0	18	theme	film	81:84	arg1	membrane					112:119	thin film composite forward osmosis membrane	76:119	thin film composite forward osmosis membrane	76:119	New insights into the organic fouling mechanism of an in situ Ca2+ modified thin film composite forward osmosis membrane.
35541777	3	19	theme	fouling	607:613	arg1	mechanism					615:623	the fouling mechanism	603:623	the fouling mechanism	603:623	Fouling experiments were carried out and the fouling mechanism was investigated by extended Derjaguin-Landau-Verwey-Overbeek (XDLVO) theory.
35541777	2	20	theme	interaction	517:527	arg1	analysis					488:495	the analysis	484:495	the analysis of foulant-membrane interaction	484:527	Bovine serum albumin (BSA), humic acid (HA) and sodium alginate (SA) were used as surrogate foulants for protein, natural organic substances and polysaccharides, respectively, thus enabling the analysis of foulant-membrane interaction in the membrane fouling process.
35541777	6	21	from	addition	971:978	arg1	membrane					991:998	the TFC membrane	983:998	the TFC membrane	983:998	In situ Ca2+ addition in the TFC membrane resulted in the decrease of the interfacial adhesion free energy (i.e., foulant-membrane interaction) and thus the mitigation of membrane fouling.
35541777	8	22	theme	antifouling	1266:1276	arg1	mechanism					1278:1286	The antifouling mechanism	1262:1286	The antifouling mechanism of Ca2+ pre-binding carboxyl groups in the TFC-Ca FO membrane	1262:1348	The antifouling mechanism of Ca2+ pre-binding carboxyl groups in the TFC-Ca FO membrane was demonstrated, which provides new insights into the development of antifouling TFC membranes in the future.
35541777	2	23	theme	sodium	342:347	arg1	polysaccharides					439:453	polysaccharides	439:453	polysaccharides	439:453	Bovine serum albumin (BSA), humic acid (HA) and sodium alginate (SA) were used as surrogate foulants for protein, natural organic substances and polysaccharides, respectively, thus enabling the analysis of foulant-membrane interaction in the membrane fouling process.
35541777	2	23	theme	sodium	342:347	arg1	acid					328:331	humic acid	322:331	humic acid (HA)	322:336	Bovine serum albumin (BSA), humic acid (HA) and sodium alginate (SA) were used as surrogate foulants for protein, natural organic substances and polysaccharides, respectively, thus enabling the analysis of foulant-membrane interaction in the membrane fouling process.
35541777	2	23	theme	sodium	342:347	arg1	alginate					349:356	sodium alginate	342:356	sodium alginate (SA)	342:361	Bovine serum albumin (BSA), humic acid (HA) and sodium alginate (SA) were used as surrogate foulants for protein, natural organic substances and polysaccharides, respectively, thus enabling the analysis of foulant-membrane interaction in the membrane fouling process.
35541777	2	23	theme	sodium	342:347	arg1	substances					424:433	natural organic substances	408:433	natural organic substances	408:433	Bovine serum albumin (BSA), humic acid (HA) and sodium alginate (SA) were used as surrogate foulants for protein, natural organic substances and polysaccharides, respectively, thus enabling the analysis of foulant-membrane interaction in the membrane fouling process.
35541777	2	23	theme	sodium	342:347	arg1	albumin					307:313	Bovine serum albumin	294:313	Bovine serum albumin (BSA)	294:319	Bovine serum albumin (BSA), humic acid (HA) and sodium alginate (SA) were used as surrogate foulants for protein, natural organic substances and polysaccharides, respectively, thus enabling the analysis of foulant-membrane interaction in the membrane fouling process.
35541777	2	23	theme	sodium	342:347	arg1	SA					359:360	SA	359:360	SA	359:360	Bovine serum albumin (BSA), humic acid (HA) and sodium alginate (SA) were used as surrogate foulants for protein, natural organic substances and polysaccharides, respectively, thus enabling the analysis of foulant-membrane interaction in the membrane fouling process.
35541777	2	23	theme	sodium	342:347	arg1	foulants					386:393	surrogate foulants	376:393	surrogate foulants for protein	376:405	Bovine serum albumin (BSA), humic acid (HA) and sodium alginate (SA) were used as surrogate foulants for protein, natural organic substances and polysaccharides, respectively, thus enabling the analysis of foulant-membrane interaction in the membrane fouling process.
35541777	3	24	theme	extended	645:652	arg1	theory					695:700	extended Derjaguin-Landau-Verwey-Overbeek (XDLVO) theory	645:700	extended Derjaguin-Landau-Verwey-Overbeek (XDLVO) theory	645:700	Fouling experiments were carried out and the fouling mechanism was investigated by extended Derjaguin-Landau-Verwey-Overbeek (XDLVO) theory.
35541777	2	25	theme	foulant-membrane	500:515	arg1	interaction					517:527	foulant-membrane interaction	500:527	foulant-membrane interaction	500:527	Bovine serum albumin (BSA), humic acid (HA) and sodium alginate (SA) were used as surrogate foulants for protein, natural organic substances and polysaccharides, respectively, thus enabling the analysis of foulant-membrane interaction in the membrane fouling process.
35541777	2	26	theme	surrogate	376:384	arg1	albumin					307:313	Bovine serum albumin	294:313	Bovine serum albumin (BSA)	294:319	Bovine serum albumin (BSA), humic acid (HA) and sodium alginate (SA) were used as surrogate foulants for protein, natural organic substances and polysaccharides, respectively, thus enabling the analysis of foulant-membrane interaction in the membrane fouling process.
35541777	2	26	theme	surrogate	376:384	arg1	acid					328:331	humic acid	322:331	humic acid (HA)	322:336	Bovine serum albumin (BSA), humic acid (HA) and sodium alginate (SA) were used as surrogate foulants for protein, natural organic substances and polysaccharides, respectively, thus enabling the analysis of foulant-membrane interaction in the membrane fouling process.
35541777	2	26	theme	surrogate	376:384	arg1	alginate					349:356	sodium alginate	342:356	sodium alginate (SA)	342:361	Bovine serum albumin (BSA), humic acid (HA) and sodium alginate (SA) were used as surrogate foulants for protein, natural organic substances and polysaccharides, respectively, thus enabling the analysis of foulant-membrane interaction in the membrane fouling process.
35541777	2	26	theme	surrogate	376:384	arg1	foulants					386:393	surrogate foulants	376:393	surrogate foulants for protein	376:405	Bovine serum albumin (BSA), humic acid (HA) and sodium alginate (SA) were used as surrogate foulants for protein, natural organic substances and polysaccharides, respectively, thus enabling the analysis of foulant-membrane interaction in the membrane fouling process.
35541777	5	27	theme	advanced	861:868	arg1	measurements					884:895	advanced contact angle measurements	861:895	advanced contact angle measurements	861:895	Results suggested that the interfacial free energies obtained from advanced contact angle measurements were correlated strongly with the rates of membrane fouling.
35541777	8	28	from	groups	1317:1322	arg1	membrane					1341:1348	the TFC-Ca FO membrane	1327:1348	the TFC-Ca FO membrane	1327:1348	The antifouling mechanism of Ca2+ pre-binding carboxyl groups in the TFC-Ca FO membrane was demonstrated, which provides new insights into the development of antifouling TFC membranes in the future.
35541777	0	29	theme	New	0:2	arg1	insights					4:11	New insights	0:11	New insights into the organic fouling mechanism of an in situ Ca2+	0:65	New insights into the organic fouling mechanism of an in situ Ca2+ modified thin film composite forward osmosis membrane.
35541777	3	30	theme	Derjaguin-Landau-Verwey-Overbeek	654:685	arg1	theory					695:700	extended Derjaguin-Landau-Verwey-Overbeek (XDLVO) theory	645:700	extended Derjaguin-Landau-Verwey-Overbeek (XDLVO) theory	645:700	Fouling experiments were carried out and the fouling mechanism was investigated by extended Derjaguin-Landau-Verwey-Overbeek (XDLVO) theory.
35541777	6	31	dep	In	958:959	arg1	situ					961:964	situ	961:964	situ	961:964	In situ Ca2+ addition in the TFC membrane resulted in the decrease of the interfacial adhesion free energy (i.e., foulant-membrane interaction) and thus the mitigation of membrane fouling.
35541777	5	32	theme	contact	870:876	arg1	measurements					884:895	advanced contact angle measurements	861:895	advanced contact angle measurements	861:895	Results suggested that the interfacial free energies obtained from advanced contact angle measurements were correlated strongly with the rates of membrane fouling.
35541777	1	33	theme	organic	151:157	arg1	substances					159:168	organic substances	151:168	organic substances	151:168	In this study, the effect of organic substances on the fouling behavior of a thin film composite (TFC) membrane with in situ Ca2+ addition (TFC-Ca membrane) was evaluated.
35541777	1	34	theme	membrane	225:232	arg1	behavior					185:192	the fouling behavior	173:192	the fouling behavior of a thin film composite (TFC) membrane with in situ Ca2+ addition (TFC-Ca membrane)	173:277	In this study, the effect of organic substances on the fouling behavior of a thin film composite (TFC) membrane with in situ Ca2+ addition (TFC-Ca membrane) was evaluated.
35541777	0	35	theme	osmosis	104:110	arg1	membrane					112:119	thin film composite forward osmosis membrane	76:119	thin film composite forward osmosis membrane	76:119	New insights into the organic fouling mechanism of an in situ Ca2+ modified thin film composite forward osmosis membrane.
35541777	1	36	with	membrane	225:232	arg1	addition					252:259	in situ Ca2+ addition	239:259	in situ Ca2+ addition (TFC-Ca membrane)	239:277	In this study, the effect of organic substances on the fouling behavior of a thin film composite (TFC) membrane with in situ Ca2+ addition (TFC-Ca membrane) was evaluated.
35541777	1	36	with	membrane	225:232	arg1	membrane					269:276	TFC-Ca membrane	262:276	TFC-Ca membrane	262:276	In this study, the effect of organic substances on the fouling behavior of a thin film composite (TFC) membrane with in situ Ca2+ addition (TFC-Ca membrane) was evaluated.
35541777	0	37	dep	in	54:55	arg1	situ					57:60	situ	57:60	situ	57:60	New insights into the organic fouling mechanism of an in situ Ca2+ modified thin film composite forward osmosis membrane.
35541777	8	38	theme	antifouling	1420:1430	arg1	membranes					1436:1444	antifouling TFC membranes	1420:1444	antifouling TFC membranes	1420:1444	The antifouling mechanism of Ca2+ pre-binding carboxyl groups in the TFC-Ca FO membrane was demonstrated, which provides new insights into the development of antifouling TFC membranes in the future.
35541777	1	39	theme	substances	159:168	arg1	effect					141:146	the effect	137:146	the effect of organic substances on the fouling behavior of a thin film composite (TFC) membrane with in situ Ca2+ addition (TFC-Ca membrane)	137:277	In this study, the effect of organic substances on the fouling behavior of a thin film composite (TFC) membrane with in situ Ca2+ addition (TFC-Ca membrane) was evaluated.
35541777	4	40	theme	fouled	770:775	arg1	membrane					784:791	the fouled TFC-Ca membrane	766:791	the fouled TFC-Ca membrane	766:791	SEM-EDX, ICP-OES and TOC analysis were applied to characterize the fouled TFC-Ca membrane.
35541777	0	41	theme	forward	96:102	arg1	membrane					112:119	thin film composite forward osmosis membrane	76:119	thin film composite forward osmosis membrane	76:119	New insights into the organic fouling mechanism of an in situ Ca2+ modified thin film composite forward osmosis membrane.
35541777	8	42	theme	Ca2+	1291:1294	arg1	groups					1317:1322	Ca2+ pre-binding carboxyl groups	1291:1322	Ca2+ pre-binding carboxyl groups in the TFC-Ca FO membrane	1291:1348	The antifouling mechanism of Ca2+ pre-binding carboxyl groups in the TFC-Ca FO membrane was demonstrated, which provides new insights into the development of antifouling TFC membranes in the future.
35541777	8	43	from	membrane	1341:1348	arg1	mechanism					1278:1286	The antifouling mechanism	1262:1286	The antifouling mechanism of Ca2+ pre-binding carboxyl groups in the TFC-Ca FO membrane	1262:1348	The antifouling mechanism of Ca2+ pre-binding carboxyl groups in the TFC-Ca FO membrane was demonstrated, which provides new insights into the development of antifouling TFC membranes in the future.
35541777	6	44	dep	interaction	1089:1099	arg1	i.e.					1066:1069	i.e.	1066:1069	i.e.	1066:1069	In situ Ca2+ addition in the TFC membrane resulted in the decrease of the interfacial adhesion free energy (i.e., foulant-membrane interaction) and thus the mitigation of membrane fouling.
35541777	0	45	theme	fouling	30:36	arg1	mechanism					38:46	the organic fouling mechanism	18:46	the organic fouling mechanism of an in situ Ca2+	18:65	New insights into the organic fouling mechanism of an in situ Ca2+ modified thin film composite forward osmosis membrane.
35541777	1	46	theme	in	239:240	arg1	addition					252:259	in situ Ca2+ addition	239:259	in situ Ca2+ addition (TFC-Ca membrane)	239:277	In this study, the effect of organic substances on the fouling behavior of a thin film composite (TFC) membrane with in situ Ca2+ addition (TFC-Ca membrane) was evaluated.
35541777	1	46	theme	in	239:240	arg1	membrane					269:276	TFC-Ca membrane	262:276	TFC-Ca membrane	262:276	In this study, the effect of organic substances on the fouling behavior of a thin film composite (TFC) membrane with in situ Ca2+ addition (TFC-Ca membrane) was evaluated.
35541777	2	47	theme	organic	416:422	arg1	substances					424:433	natural organic substances	408:433	natural organic substances	408:433	Bovine serum albumin (BSA), humic acid (HA) and sodium alginate (SA) were used as surrogate foulants for protein, natural organic substances and polysaccharides, respectively, thus enabling the analysis of foulant-membrane interaction in the membrane fouling process.
35541777	2	47	theme	organic	416:422	arg1	albumin					307:313	Bovine serum albumin	294:313	Bovine serum albumin (BSA)	294:319	Bovine serum albumin (BSA), humic acid (HA) and sodium alginate (SA) were used as surrogate foulants for protein, natural organic substances and polysaccharides, respectively, thus enabling the analysis of foulant-membrane interaction in the membrane fouling process.
35541777	2	47	theme	organic	416:422	arg1	acid					328:331	humic acid	322:331	humic acid (HA)	322:336	Bovine serum albumin (BSA), humic acid (HA) and sodium alginate (SA) were used as surrogate foulants for protein, natural organic substances and polysaccharides, respectively, thus enabling the analysis of foulant-membrane interaction in the membrane fouling process.
35541777	2	47	theme	organic	416:422	arg1	alginate					349:356	sodium alginate	342:356	sodium alginate (SA)	342:361	Bovine serum albumin (BSA), humic acid (HA) and sodium alginate (SA) were used as surrogate foulants for protein, natural organic substances and polysaccharides, respectively, thus enabling the analysis of foulant-membrane interaction in the membrane fouling process.
35541777	7	48	theme	TFC-Ca	1168:1173	arg1	membrane					1178:1185	TFC-Ca FO membrane	1168:1185	TFC-Ca FO membrane	1168:1185	The permeate flux of TFC-Ca FO membrane after organic fouling could be fully restored by simple physical cleaning.
35541777	5	49	theme	fouling	949:955	arg1	rates					931:935	the rates	927:935	the rates of membrane fouling	927:955	Results suggested that the interfacial free energies obtained from advanced contact angle measurements were correlated strongly with the rates of membrane fouling.
35541777	6	50	theme	TFC	987:989	arg1	membrane					991:998	the TFC membrane	983:998	the TFC membrane	983:998	In situ Ca2+ addition in the TFC membrane resulted in the decrease of the interfacial adhesion free energy (i.e., foulant-membrane interaction) and thus the mitigation of membrane fouling.
35541777	2	51	theme	humic	322:326	arg1	HA					334:335	HA	334:335	HA	334:335	Bovine serum albumin (BSA), humic acid (HA) and sodium alginate (SA) were used as surrogate foulants for protein, natural organic substances and polysaccharides, respectively, thus enabling the analysis of foulant-membrane interaction in the membrane fouling process.
35541777	2	51	theme	humic	322:326	arg1	polysaccharides					439:453	polysaccharides	439:453	polysaccharides	439:453	Bovine serum albumin (BSA), humic acid (HA) and sodium alginate (SA) were used as surrogate foulants for protein, natural organic substances and polysaccharides, respectively, thus enabling the analysis of foulant-membrane interaction in the membrane fouling process.
35541777	2	51	theme	humic	322:326	arg1	acid					328:331	humic acid	322:331	humic acid (HA)	322:336	Bovine serum albumin (BSA), humic acid (HA) and sodium alginate (SA) were used as surrogate foulants for protein, natural organic substances and polysaccharides, respectively, thus enabling the analysis of foulant-membrane interaction in the membrane fouling process.
35541777	2	51	theme	humic	322:326	arg1	alginate					349:356	sodium alginate	342:356	sodium alginate (SA)	342:361	Bovine serum albumin (BSA), humic acid (HA) and sodium alginate (SA) were used as surrogate foulants for protein, natural organic substances and polysaccharides, respectively, thus enabling the analysis of foulant-membrane interaction in the membrane fouling process.
35541777	2	51	theme	humic	322:326	arg1	substances					424:433	natural organic substances	408:433	natural organic substances	408:433	Bovine serum albumin (BSA), humic acid (HA) and sodium alginate (SA) were used as surrogate foulants for protein, natural organic substances and polysaccharides, respectively, thus enabling the analysis of foulant-membrane interaction in the membrane fouling process.
35541777	2	51	theme	humic	322:326	arg1	albumin					307:313	Bovine serum albumin	294:313	Bovine serum albumin (BSA)	294:319	Bovine serum albumin (BSA), humic acid (HA) and sodium alginate (SA) were used as surrogate foulants for protein, natural organic substances and polysaccharides, respectively, thus enabling the analysis of foulant-membrane interaction in the membrane fouling process.
35541777	2	51	theme	humic	322:326	arg1	foulants					386:393	surrogate foulants	376:393	surrogate foulants for protein	376:405	Bovine serum albumin (BSA), humic acid (HA) and sodium alginate (SA) were used as surrogate foulants for protein, natural organic substances and polysaccharides, respectively, thus enabling the analysis of foulant-membrane interaction in the membrane fouling process.
35541777	0	52	theme	organic	22:28	arg1	mechanism					38:46	the organic fouling mechanism	18:46	the organic fouling mechanism of an in situ Ca2+	18:65	New insights into the organic fouling mechanism of an in situ Ca2+ modified thin film composite forward osmosis membrane.
35541777	2	53	theme	natural	408:414	arg1	substances					424:433	natural organic substances	408:433	natural organic substances	408:433	Bovine serum albumin (BSA), humic acid (HA) and sodium alginate (SA) were used as surrogate foulants for protein, natural organic substances and polysaccharides, respectively, thus enabling the analysis of foulant-membrane interaction in the membrane fouling process.
35541777	2	53	theme	natural	408:414	arg1	albumin					307:313	Bovine serum albumin	294:313	Bovine serum albumin (BSA)	294:319	Bovine serum albumin (BSA), humic acid (HA) and sodium alginate (SA) were used as surrogate foulants for protein, natural organic substances and polysaccharides, respectively, thus enabling the analysis of foulant-membrane interaction in the membrane fouling process.
35541777	2	53	theme	natural	408:414	arg1	acid					328:331	humic acid	322:331	humic acid (HA)	322:336	Bovine serum albumin (BSA), humic acid (HA) and sodium alginate (SA) were used as surrogate foulants for protein, natural organic substances and polysaccharides, respectively, thus enabling the analysis of foulant-membrane interaction in the membrane fouling process.
35541777	2	53	theme	natural	408:414	arg1	alginate					349:356	sodium alginate	342:356	sodium alginate (SA)	342:361	Bovine serum albumin (BSA), humic acid (HA) and sodium alginate (SA) were used as surrogate foulants for protein, natural organic substances and polysaccharides, respectively, thus enabling the analysis of foulant-membrane interaction in the membrane fouling process.
35541777	8	54	theme	new	1383:1385	arg1	insights					1387:1394	new insights	1383:1394	new insights into the development of antifouling TFC membranes	1383:1444	The antifouling mechanism of Ca2+ pre-binding carboxyl groups in the TFC-Ca FO membrane was demonstrated, which provides new insights into the development of antifouling TFC membranes in the future.
35541777	4	55	theme	TOC	724:726	arg1	analysis					728:735	TOC analysis	724:735	TOC analysis	724:735	SEM-EDX, ICP-OES and TOC analysis were applied to characterize the fouled TFC-Ca membrane.
35541777	1	56	theme	fouling	177:183	arg1	behavior					185:192	the fouling behavior	173:192	the fouling behavior of a thin film composite (TFC) membrane with in situ Ca2+ addition (TFC-Ca membrane)	173:277	In this study, the effect of organic substances on the fouling behavior of a thin film composite (TFC) membrane with in situ Ca2+ addition (TFC-Ca membrane) was evaluated.
35541777	1	57	theme	Ca2+	247:250	arg1	addition					252:259	in situ Ca2+ addition	239:259	in situ Ca2+ addition (TFC-Ca membrane)	239:277	In this study, the effect of organic substances on the fouling behavior of a thin film composite (TFC) membrane with in situ Ca2+ addition (TFC-Ca membrane) was evaluated.
35541777	1	57	theme	Ca2+	247:250	arg1	membrane					269:276	TFC-Ca membrane	262:276	TFC-Ca membrane	262:276	In this study, the effect of organic substances on the fouling behavior of a thin film composite (TFC) membrane with in situ Ca2+ addition (TFC-Ca membrane) was evaluated.
35541777	6	58	theme	Ca2+	966:969	arg1	addition					971:978	In situ Ca2+ addition	958:978	In situ Ca2+ addition in the TFC membrane	958:998	In situ Ca2+ addition in the TFC membrane resulted in the decrease of the interfacial adhesion free energy (i.e., foulant-membrane interaction) and thus the mitigation of membrane fouling.
35541777	8	59	theme	TFC-Ca	1331:1336	arg1	membrane					1341:1348	the TFC-Ca FO membrane	1327:1348	the TFC-Ca FO membrane	1327:1348	The antifouling mechanism of Ca2+ pre-binding carboxyl groups in the TFC-Ca FO membrane was demonstrated, which provides new insights into the development of antifouling TFC membranes in the future.
35541777	5	60	theme	free	833:836	arg1	energies					838:845	the interfacial free energies	817:845	the interfacial free energies obtained from advanced contact angle measurements	817:895	Results suggested that the interfacial free energies obtained from advanced contact angle measurements were correlated strongly with the rates of membrane fouling.
35541777	7	61	theme	simple	1236:1241	arg1	cleaning					1252:1259	simple physical cleaning	1236:1259	simple physical cleaning	1236:1259	The permeate flux of TFC-Ca FO membrane after organic fouling could be fully restored by simple physical cleaning.
35541777	6	62	theme	fouling	1138:1144	arg1	mitigation					1115:1124	the mitigation	1111:1124	the mitigation of membrane fouling	1111:1144	In situ Ca2+ addition in the TFC membrane resulted in the decrease of the interfacial adhesion free energy (i.e., foulant-membrane interaction) and thus the mitigation of membrane fouling.
35541777	7	63	theme	organic	1193:1199	arg1	fouling					1201:1207	organic fouling	1193:1207	organic fouling	1193:1207	The permeate flux of TFC-Ca FO membrane after organic fouling could be fully restored by simple physical cleaning.
35541777	6	64	theme	free	1053:1056	arg1	interaction					1089:1099	foulant-membrane interaction	1072:1099	foulant-membrane interaction	1072:1099	In situ Ca2+ addition in the TFC membrane resulted in the decrease of the interfacial adhesion free energy (i.e., foulant-membrane interaction) and thus the mitigation of membrane fouling.
35541777	6	64	theme	free	1053:1056	arg1	energy					1058:1063	the interfacial adhesion free energy	1028:1063	the interfacial adhesion free energy (i.e., foulant-membrane interaction)	1028:1100	In situ Ca2+ addition in the TFC membrane resulted in the decrease of the interfacial adhesion free energy (i.e., foulant-membrane interaction) and thus the mitigation of membrane fouling.
35541777	8	65	from	mechanism	1278:1286	arg1	membrane					1341:1348	the TFC-Ca FO membrane	1327:1348	the TFC-Ca FO membrane	1327:1348	The antifouling mechanism of Ca2+ pre-binding carboxyl groups in the TFC-Ca FO membrane was demonstrated, which provides new insights into the development of antifouling TFC membranes in the future.
35541777	7	66	theme	FO	1175:1176	arg1	membrane					1178:1185	TFC-Ca FO membrane	1168:1185	TFC-Ca FO membrane	1168:1185	The permeate flux of TFC-Ca FO membrane after organic fouling could be fully restored by simple physical cleaning.
35541777	2	67	used	used	368:371	arg2	acid					328:331	humic acid	322:331	humic acid (HA)	322:336	Bovine serum albumin (BSA), humic acid (HA) and sodium alginate (SA) were used as surrogate foulants for protein, natural organic substances and polysaccharides, respectively, thus enabling the analysis of foulant-membrane interaction in the membrane fouling process.
35541777	2	67	used	used	368:371	arg2	alginate					349:356	sodium alginate	342:356	sodium alginate (SA)	342:361	Bovine serum albumin (BSA), humic acid (HA) and sodium alginate (SA) were used as surrogate foulants for protein, natural organic substances and polysaccharides, respectively, thus enabling the analysis of foulant-membrane interaction in the membrane fouling process.
35541777	2	67	used	used	368:371	arg2	SA					359:360	SA	359:360	SA	359:360	Bovine serum albumin (BSA), humic acid (HA) and sodium alginate (SA) were used as surrogate foulants for protein, natural organic substances and polysaccharides, respectively, thus enabling the analysis of foulant-membrane interaction in the membrane fouling process.
35541777	2	67	used	used	368:371	arg2	foulants					386:393	surrogate foulants	376:393	surrogate foulants for protein	376:405	Bovine serum albumin (BSA), humic acid (HA) and sodium alginate (SA) were used as surrogate foulants for protein, natural organic substances and polysaccharides, respectively, thus enabling the analysis of foulant-membrane interaction in the membrane fouling process.
35541777	2	67	used	used	368:371	arg2	substances					424:433	natural organic substances	408:433	natural organic substances	408:433	Bovine serum albumin (BSA), humic acid (HA) and sodium alginate (SA) were used as surrogate foulants for protein, natural organic substances and polysaccharides, respectively, thus enabling the analysis of foulant-membrane interaction in the membrane fouling process.
35541777	2	67	used	used	368:371	arg2	albumin					307:313	Bovine serum albumin	294:313	Bovine serum albumin (BSA)	294:319	Bovine serum albumin (BSA), humic acid (HA) and sodium alginate (SA) were used as surrogate foulants for protein, natural organic substances and polysaccharides, respectively, thus enabling the analysis of foulant-membrane interaction in the membrane fouling process.
35541777	2	67	used	used	368:371	arg2	polysaccharides					439:453	polysaccharides	439:453	polysaccharides	439:453	Bovine serum albumin (BSA), humic acid (HA) and sodium alginate (SA) were used as surrogate foulants for protein, natural organic substances and polysaccharides, respectively, thus enabling the analysis of foulant-membrane interaction in the membrane fouling process.
35541777	2	67	used	used	368:371	arg2	BSA					316:318	BSA	316:318	BSA	316:318	Bovine serum albumin (BSA), humic acid (HA) and sodium alginate (SA) were used as surrogate foulants for protein, natural organic substances and polysaccharides, respectively, thus enabling the analysis of foulant-membrane interaction in the membrane fouling process.
35541777	2	67	used	used	368:371	arg2	HA					334:335	HA	334:335	HA	334:335	Bovine serum albumin (BSA), humic acid (HA) and sodium alginate (SA) were used as surrogate foulants for protein, natural organic substances and polysaccharides, respectively, thus enabling the analysis of foulant-membrane interaction in the membrane fouling process.
35541777	6	68	theme	membrane	1129:1136	arg1	fouling					1138:1144	membrane fouling	1129:1144	membrane fouling	1129:1144	In situ Ca2+ addition in the TFC membrane resulted in the decrease of the interfacial adhesion free energy (i.e., foulant-membrane interaction) and thus the mitigation of membrane fouling.
35541777	8	69	theme	groups	1317:1322	arg1	mechanism					1278:1286	The antifouling mechanism	1262:1286	The antifouling mechanism of Ca2+ pre-binding carboxyl groups in the TFC-Ca FO membrane	1262:1348	The antifouling mechanism of Ca2+ pre-binding carboxyl groups in the TFC-Ca FO membrane was demonstrated, which provides new insights into the development of antifouling TFC membranes in the future.
35541777	6	70	theme	adhesion	1044:1051	arg1	interaction					1089:1099	foulant-membrane interaction	1072:1099	foulant-membrane interaction	1072:1099	In situ Ca2+ addition in the TFC membrane resulted in the decrease of the interfacial adhesion free energy (i.e., foulant-membrane interaction) and thus the mitigation of membrane fouling.
35541777	6	70	theme	adhesion	1044:1051	arg1	energy					1058:1063	the interfacial adhesion free energy	1028:1063	the interfacial adhesion free energy (i.e., foulant-membrane interaction)	1028:1100	In situ Ca2+ addition in the TFC membrane resulted in the decrease of the interfacial adhesion free energy (i.e., foulant-membrane interaction) and thus the mitigation of membrane fouling.
35541777	5	71	theme	angle	878:882	arg1	measurements					884:895	advanced contact angle measurements	861:895	advanced contact angle measurements	861:895	Results suggested that the interfacial free energies obtained from advanced contact angle measurements were correlated strongly with the rates of membrane fouling.
35541777	8	72	theme	FO	1338:1339	arg1	membrane					1341:1348	the TFC-Ca FO membrane	1327:1348	the TFC-Ca FO membrane	1327:1348	The antifouling mechanism of Ca2+ pre-binding carboxyl groups in the TFC-Ca FO membrane was demonstrated, which provides new insights into the development of antifouling TFC membranes in the future.
35541777	6	73	theme	interfacial	1032:1042	arg1	interaction					1089:1099	foulant-membrane interaction	1072:1099	foulant-membrane interaction	1072:1099	In situ Ca2+ addition in the TFC membrane resulted in the decrease of the interfacial adhesion free energy (i.e., foulant-membrane interaction) and thus the mitigation of membrane fouling.
35541777	6	73	theme	interfacial	1032:1042	arg1	energy					1058:1063	the interfacial adhesion free energy	1028:1063	the interfacial adhesion free energy (i.e., foulant-membrane interaction)	1028:1100	In situ Ca2+ addition in the TFC membrane resulted in the decrease of the interfacial adhesion free energy (i.e., foulant-membrane interaction) and thus the mitigation of membrane fouling.
35541777	6	74	theme	In	958:959	arg1	addition					971:978	In situ Ca2+ addition	958:978	In situ Ca2+ addition in the TFC membrane	958:998	In situ Ca2+ addition in the TFC membrane resulted in the decrease of the interfacial adhesion free energy (i.e., foulant-membrane interaction) and thus the mitigation of membrane fouling.
35541777	0	75	theme	in	54:55	arg1	Ca2+					62:65	an in situ Ca2+	51:65	an in situ Ca2+	51:65	New insights into the organic fouling mechanism of an in situ Ca2+ modified thin film composite forward osmosis membrane.
35541777	3	76	theme	Fouling	562:568	arg1	experiments					570:580	Fouling experiments	562:580	Fouling experiments	562:580	Fouling experiments were carried out and the fouling mechanism was investigated by extended Derjaguin-Landau-Verwey-Overbeek (XDLVO) theory.
35541777	2	77	theme	serum	301:305	arg1	BSA					316:318	BSA	316:318	BSA	316:318	Bovine serum albumin (BSA), humic acid (HA) and sodium alginate (SA) were used as surrogate foulants for protein, natural organic substances and polysaccharides, respectively, thus enabling the analysis of foulant-membrane interaction in the membrane fouling process.
35541777	2	77	theme	serum	301:305	arg1	polysaccharides					439:453	polysaccharides	439:453	polysaccharides	439:453	Bovine serum albumin (BSA), humic acid (HA) and sodium alginate (SA) were used as surrogate foulants for protein, natural organic substances and polysaccharides, respectively, thus enabling the analysis of foulant-membrane interaction in the membrane fouling process.
35541777	2	77	theme	serum	301:305	arg1	acid					328:331	humic acid	322:331	humic acid (HA)	322:336	Bovine serum albumin (BSA), humic acid (HA) and sodium alginate (SA) were used as surrogate foulants for protein, natural organic substances and polysaccharides, respectively, thus enabling the analysis of foulant-membrane interaction in the membrane fouling process.
35541777	2	77	theme	serum	301:305	arg1	alginate					349:356	sodium alginate	342:356	sodium alginate (SA)	342:361	Bovine serum albumin (BSA), humic acid (HA) and sodium alginate (SA) were used as surrogate foulants for protein, natural organic substances and polysaccharides, respectively, thus enabling the analysis of foulant-membrane interaction in the membrane fouling process.
35541777	2	77	theme	serum	301:305	arg1	substances					424:433	natural organic substances	408:433	natural organic substances	408:433	Bovine serum albumin (BSA), humic acid (HA) and sodium alginate (SA) were used as surrogate foulants for protein, natural organic substances and polysaccharides, respectively, thus enabling the analysis of foulant-membrane interaction in the membrane fouling process.
35541777	2	77	theme	serum	301:305	arg1	albumin					307:313	Bovine serum albumin	294:313	Bovine serum albumin (BSA)	294:319	Bovine serum albumin (BSA), humic acid (HA) and sodium alginate (SA) were used as surrogate foulants for protein, natural organic substances and polysaccharides, respectively, thus enabling the analysis of foulant-membrane interaction in the membrane fouling process.
35541777	2	77	theme	serum	301:305	arg1	foulants					386:393	surrogate foulants	376:393	surrogate foulants for protein	376:405	Bovine serum albumin (BSA), humic acid (HA) and sodium alginate (SA) were used as surrogate foulants for protein, natural organic substances and polysaccharides, respectively, thus enabling the analysis of foulant-membrane interaction in the membrane fouling process.
35541777	8	78	theme	carboxyl	1308:1315	arg1	groups					1317:1322	Ca2+ pre-binding carboxyl groups	1291:1322	Ca2+ pre-binding carboxyl groups in the TFC-Ca FO membrane	1291:1348	The antifouling mechanism of Ca2+ pre-binding carboxyl groups in the TFC-Ca FO membrane was demonstrated, which provides new insights into the development of antifouling TFC membranes in the future.
35541777	7	79	theme	permeate	1151:1158	arg1	flux					1160:1163	The permeate flux	1147:1163	The permeate flux of TFC-Ca FO membrane after organic fouling	1147:1207	The permeate flux of TFC-Ca FO membrane after organic fouling could be fully restored by simple physical cleaning.
35541777	1	80	theme	TFC-Ca	262:267	arg1	addition					252:259	in situ Ca2+ addition	239:259	in situ Ca2+ addition (TFC-Ca membrane)	239:277	In this study, the effect of organic substances on the fouling behavior of a thin film composite (TFC) membrane with in situ Ca2+ addition (TFC-Ca membrane) was evaluated.
35541777	1	80	theme	TFC-Ca	262:267	arg1	membrane					269:276	TFC-Ca membrane	262:276	TFC-Ca membrane	262:276	In this study, the effect of organic substances on the fouling behavior of a thin film composite (TFC) membrane with in situ Ca2+ addition (TFC-Ca membrane) was evaluated.
35541777	5	81	theme	membrane	940:947	arg1	fouling					949:955	membrane fouling	940:955	membrane fouling	940:955	Results suggested that the interfacial free energies obtained from advanced contact angle measurements were correlated strongly with the rates of membrane fouling.
35541777	7	82	theme	membrane	1178:1185	arg1	flux					1160:1163	The permeate flux	1147:1163	The permeate flux of TFC-Ca FO membrane after organic fouling	1147:1207	The permeate flux of TFC-Ca FO membrane after organic fouling could be fully restored by simple physical cleaning.
35541777	2	83	theme	Bovine	294:299	arg1	BSA					316:318	BSA	316:318	BSA	316:318	Bovine serum albumin (BSA), humic acid (HA) and sodium alginate (SA) were used as surrogate foulants for protein, natural organic substances and polysaccharides, respectively, thus enabling the analysis of foulant-membrane interaction in the membrane fouling process.
35541777	2	83	theme	Bovine	294:299	arg1	polysaccharides					439:453	polysaccharides	439:453	polysaccharides	439:453	Bovine serum albumin (BSA), humic acid (HA) and sodium alginate (SA) were used as surrogate foulants for protein, natural organic substances and polysaccharides, respectively, thus enabling the analysis of foulant-membrane interaction in the membrane fouling process.
35541777	2	83	theme	Bovine	294:299	arg1	acid					328:331	humic acid	322:331	humic acid (HA)	322:336	Bovine serum albumin (BSA), humic acid (HA) and sodium alginate (SA) were used as surrogate foulants for protein, natural organic substances and polysaccharides, respectively, thus enabling the analysis of foulant-membrane interaction in the membrane fouling process.
35541777	2	83	theme	Bovine	294:299	arg1	alginate					349:356	sodium alginate	342:356	sodium alginate (SA)	342:361	Bovine serum albumin (BSA), humic acid (HA) and sodium alginate (SA) were used as surrogate foulants for protein, natural organic substances and polysaccharides, respectively, thus enabling the analysis of foulant-membrane interaction in the membrane fouling process.
35541777	2	83	theme	Bovine	294:299	arg1	substances					424:433	natural organic substances	408:433	natural organic substances	408:433	Bovine serum albumin (BSA), humic acid (HA) and sodium alginate (SA) were used as surrogate foulants for protein, natural organic substances and polysaccharides, respectively, thus enabling the analysis of foulant-membrane interaction in the membrane fouling process.
35541777	2	83	theme	Bovine	294:299	arg1	albumin					307:313	Bovine serum albumin	294:313	Bovine serum albumin (BSA)	294:319	Bovine serum albumin (BSA), humic acid (HA) and sodium alginate (SA) were used as surrogate foulants for protein, natural organic substances and polysaccharides, respectively, thus enabling the analysis of foulant-membrane interaction in the membrane fouling process.
35541777	2	83	theme	Bovine	294:299	arg1	foulants					386:393	surrogate foulants	376:393	surrogate foulants for protein	376:405	Bovine serum albumin (BSA), humic acid (HA) and sodium alginate (SA) were used as surrogate foulants for protein, natural organic substances and polysaccharides, respectively, thus enabling the analysis of foulant-membrane interaction in the membrane fouling process.
35541777	1	84	theme	thin	199:202	arg1	film					204:207	a thin film	197:207	a thin film composite (TFC) membrane with in situ Ca2+ addition (TFC-Ca membrane)	197:277	In this study, the effect of organic substances on the fouling behavior of a thin film composite (TFC) membrane with in situ Ca2+ addition (TFC-Ca membrane) was evaluated.
30086833	0	0	theme	cationic	78:85	arg1	tags					87:90	cationic tags	78:90	cationic tags	78:90	Investigation of a side reaction occurring during N-linked glycan labeling by cationic tags.
30086833	5	1	theme	sensitive	1066:1074	arg1	analyses					1086:1093	high sensitive CE-ESI/MS analyses	1061:1093	high sensitive CE-ESI/MS analyses of N-linked glycans	1061:1113	Finally, it was confirmed that derivatization of sugars via a hydrazone formation can be a simpler method with a high reaction yield suitable for high sensitive CE-ESI/MS analyses of N-linked glycans.
30086833	3	2	theme	ion	562:564	arg1	mode					566:569	a positive ion mode	551:569	a positive ion mode	551:569	However, a formation of multiple labeled N-linked glycans was observed by CE-MS in a positive ion mode when positively charged labels such as aliphatic amines containing a quaternary ammonium group were attached to N-linked glycans by reductive amination.
30086833	1	3	theme	electrospray	280:291	arg1	efficiency					304:313	electrospray ionization efficiency	280:313	electrospray ionization efficiency	280:313	Capillary electrophoresis-mass spectrometry was applied for the analysis of oligosaccharides and N-linked glycans with an attached charge label facilitating electrophoretic migration and electrospray ionization efficiency.
30086833	5	4	theme	reaction	1033:1040	arg1	yield					1042:1046	a high reaction yield	1026:1046	a high reaction yield suitable for high sensitive CE-ESI/MS analyses of N-linked glycans	1026:1113	Finally, it was confirmed that derivatization of sugars via a hydrazone formation can be a simpler method with a high reaction yield suitable for high sensitive CE-ESI/MS analyses of N-linked glycans.
30086833	1	5	with	oligosaccharides	169:184	arg1	charge					224:229	an attached charge	212:229	an attached charge label facilitating electrophoretic migration and electrospray ionization efficiency	212:313	Capillary electrophoresis-mass spectrometry was applied for the analysis of oligosaccharides and N-linked glycans with an attached charge label facilitating electrophoretic migration and electrospray ionization efficiency.
30086833	1	6	theme	oligosaccharides	169:184	arg1	analysis					157:164	the analysis	153:164	the analysis of oligosaccharides and N-linked glycans with an attached charge label facilitating electrophoretic migration and electrospray ionization efficiency	153:313	Capillary electrophoresis-mass spectrometry was applied for the analysis of oligosaccharides and N-linked glycans with an attached charge label facilitating electrophoretic migration and electrospray ionization efficiency.
30086833	1	7	theme	ionization	293:302	arg1	efficiency					304:313	electrospray ionization efficiency	280:313	electrospray ionization efficiency	280:313	Capillary electrophoresis-mass spectrometry was applied for the analysis of oligosaccharides and N-linked glycans with an attached charge label facilitating electrophoretic migration and electrospray ionization efficiency.
30086833	5	8	link	N-linked	1098:1105	arg1	glycans					1107:1113	N-linked glycans	1098:1113	N-linked glycans	1098:1113	Finally, it was confirmed that derivatization of sugars via a hydrazone formation can be a simpler method with a high reaction yield suitable for high sensitive CE-ESI/MS analyses of N-linked glycans.
30086833	3	9	located	observed	530:537	arg2	formation					479:487	a formation	477:487	a formation of multiple labeled N-linked glycans	477:524	However, a formation of multiple labeled N-linked glycans was observed by CE-MS in a positive ion mode when positively charged labels such as aliphatic amines containing a quaternary ammonium group were attached to N-linked glycans by reductive amination.
30086833	3	9	located	observed	530:537	arg1	mode					566:569	a positive ion mode	551:569	a positive ion mode	551:569	However, a formation of multiple labeled N-linked glycans was observed by CE-MS in a positive ion mode when positively charged labels such as aliphatic amines containing a quaternary ammonium group were attached to N-linked glycans by reductive amination.
30086833	5	10	theme	hydrazone	977:985	arg1	formation					987:995	a hydrazone formation	975:995	a hydrazone formation	975:995	Finally, it was confirmed that derivatization of sugars via a hydrazone formation can be a simpler method with a high reaction yield suitable for high sensitive CE-ESI/MS analyses of N-linked glycans.
30086833	4	11	theme	reaction	726:733	arg1	mechanism					735:743	A reaction mechanism	724:743	A reaction mechanism explaining a side reaction occurring during the labeling	724:800	A reaction mechanism explaining a side reaction occurring during the labeling and the multiple product formation was proposed and confirmed by using isotopically labeled N-acetylglucosamine.
30086833	5	12	theme	suitable	1048:1055	arg1	yield					1042:1046	a high reaction yield	1026:1046	a high reaction yield suitable for high sensitive CE-ESI/MS analyses of N-linked glycans	1026:1113	Finally, it was confirmed that derivatization of sugars via a hydrazone formation can be a simpler method with a high reaction yield suitable for high sensitive CE-ESI/MS analyses of N-linked glycans.
30086833	2	13	theme	labeling	334:341	arg1	strategies					343:352	Several different labeling strategies	316:352	Several different labeling strategies	316:352	Several different labeling strategies have been tested with different tags and tagging reactions including reductive amination and hydrazone formation.
30086833	2	14	theme	different	324:332	arg1	strategies					343:352	Several different labeling strategies	316:352	Several different labeling strategies	316:352	Several different labeling strategies have been tested with different tags and tagging reactions including reductive amination and hydrazone formation.
30086833	2	15	theme	hydrazone	447:455	arg1	formation					457:465	hydrazone formation	447:465	hydrazone formation	447:465	Several different labeling strategies have been tested with different tags and tagging reactions including reductive amination and hydrazone formation.
30086833	3	16	theme	labeled	501:507	arg1	glycans					518:524	multiple labeled N-linked glycans	492:524	multiple labeled N-linked glycans	492:524	However, a formation of multiple labeled N-linked glycans was observed by CE-MS in a positive ion mode when positively charged labels such as aliphatic amines containing a quaternary ammonium group were attached to N-linked glycans by reductive amination.
30086833	5	17	with	method	1014:1019	arg1	yield					1042:1046	a high reaction yield	1026:1046	a high reaction yield suitable for high sensitive CE-ESI/MS analyses of N-linked glycans	1026:1113	Finally, it was confirmed that derivatization of sugars via a hydrazone formation can be a simpler method with a high reaction yield suitable for high sensitive CE-ESI/MS analyses of N-linked glycans.
30086833	0	18	link	N-linked	50:57	arg1	labeling					66:73	N-linked glycan labeling	50:73	N-linked glycan labeling by cationic tags	50:90	Investigation of a side reaction occurring during N-linked glycan labeling by cationic tags.
30086833	2	19	theme	Several	316:322	arg1	strategies					343:352	Several different labeling strategies	316:352	Several different labeling strategies	316:352	Several different labeling strategies have been tested with different tags and tagging reactions including reductive amination and hydrazone formation.
30086833	1	20	theme	N-linked	190:197	arg1	glycans					199:205	N-linked glycans	190:205	N-linked glycans	190:205	Capillary electrophoresis-mass spectrometry was applied for the analysis of oligosaccharides and N-linked glycans with an attached charge label facilitating electrophoretic migration and electrospray ionization efficiency.
30086833	4	21	theme	multiple	810:817	arg1	formation					827:835	the multiple product formation	806:835	the multiple product formation	806:835	A reaction mechanism explaining a side reaction occurring during the labeling and the multiple product formation was proposed and confirmed by using isotopically labeled N-acetylglucosamine.
30086833	1	22	theme	glycans	199:205	arg1	analysis					157:164	the analysis	153:164	the analysis of oligosaccharides and N-linked glycans with an attached charge label facilitating electrophoretic migration and electrospray ionization efficiency	153:313	Capillary electrophoresis-mass spectrometry was applied for the analysis of oligosaccharides and N-linked glycans with an attached charge label facilitating electrophoretic migration and electrospray ionization efficiency.
30086833	1	23	link	N-linked	190:197	arg1	glycans					199:205	N-linked glycans	190:205	N-linked glycans	190:205	Capillary electrophoresis-mass spectrometry was applied for the analysis of oligosaccharides and N-linked glycans with an attached charge label facilitating electrophoretic migration and electrospray ionization efficiency.
30086833	3	24	theme	multiple	492:499	arg1	glycans					518:524	multiple labeled N-linked glycans	492:524	multiple labeled N-linked glycans	492:524	However, a formation of multiple labeled N-linked glycans was observed by CE-MS in a positive ion mode when positively charged labels such as aliphatic amines containing a quaternary ammonium group were attached to N-linked glycans by reductive amination.
30086833	0	25	theme	side	19:22	arg1	reaction					24:31	a side reaction	17:31	a side reaction	17:31	Investigation of a side reaction occurring during N-linked glycan labeling by cationic tags.
30086833	1	26	theme	Capillary	93:101	arg1	spectrometry					124:135	Capillary electrophoresis-mass spectrometry	93:135	Capillary electrophoresis-mass spectrometry	93:135	Capillary electrophoresis-mass spectrometry was applied for the analysis of oligosaccharides and N-linked glycans with an attached charge label facilitating electrophoretic migration and electrospray ionization efficiency.
30086833	3	27	theme	charged	587:593	arg1	amines					620:625	aliphatic amines	610:625	aliphatic amines containing a quaternary ammonium group	610:664	However, a formation of multiple labeled N-linked glycans was observed by CE-MS in a positive ion mode when positively charged labels such as aliphatic amines containing a quaternary ammonium group were attached to N-linked glycans by reductive amination.
30086833	3	27	theme	charged	587:593	arg1	labels					595:600	positively charged labels	576:600	positively charged labels such as aliphatic amines containing a quaternary ammonium group	576:664	However, a formation of multiple labeled N-linked glycans was observed by CE-MS in a positive ion mode when positively charged labels such as aliphatic amines containing a quaternary ammonium group were attached to N-linked glycans by reductive amination.
30086833	1	28	theme	electrophoresis-mass	103:122	arg1	spectrometry					124:135	Capillary electrophoresis-mass spectrometry	93:135	Capillary electrophoresis-mass spectrometry	93:135	Capillary electrophoresis-mass spectrometry was applied for the analysis of oligosaccharides and N-linked glycans with an attached charge label facilitating electrophoretic migration and electrospray ionization efficiency.
30086833	1	29	theme	attached	215:222	arg1	charge					224:229	an attached charge	212:229	an attached charge label facilitating electrophoretic migration and electrospray ionization efficiency	212:313	Capillary electrophoresis-mass spectrometry was applied for the analysis of oligosaccharides and N-linked glycans with an attached charge label facilitating electrophoretic migration and electrospray ionization efficiency.
30086833	3	30	contain	containing	627:636	arg1	amines					620:625	aliphatic amines	610:625	aliphatic amines containing a quaternary ammonium group	610:664	However, a formation of multiple labeled N-linked glycans was observed by CE-MS in a positive ion mode when positively charged labels such as aliphatic amines containing a quaternary ammonium group were attached to N-linked glycans by reductive amination.
30086833	3	30	contain	containing	627:636	arg2	group					660:664	a quaternary ammonium group	638:664	a quaternary ammonium group	638:664	However, a formation of multiple labeled N-linked glycans was observed by CE-MS in a positive ion mode when positively charged labels such as aliphatic amines containing a quaternary ammonium group were attached to N-linked glycans by reductive amination.
30086833	3	31	link	N-linked	509:516	arg1	glycans					518:524	multiple labeled N-linked glycans	492:524	multiple labeled N-linked glycans	492:524	However, a formation of multiple labeled N-linked glycans was observed by CE-MS in a positive ion mode when positively charged labels such as aliphatic amines containing a quaternary ammonium group were attached to N-linked glycans by reductive amination.
30086833	5	32	theme	N-linked	1098:1105	arg1	glycans					1107:1113	N-linked glycans	1098:1113	N-linked glycans	1098:1113	Finally, it was confirmed that derivatization of sugars via a hydrazone formation can be a simpler method with a high reaction yield suitable for high sensitive CE-ESI/MS analyses of N-linked glycans.
30086833	3	33	theme	glycans	518:524	arg1	formation					479:487	a formation	477:487	a formation of multiple labeled N-linked glycans	477:524	However, a formation of multiple labeled N-linked glycans was observed by CE-MS in a positive ion mode when positively charged labels such as aliphatic amines containing a quaternary ammonium group were attached to N-linked glycans by reductive amination.
30086833	0	34	theme	reaction	24:31	arg1	Investigation					0:12	Investigation	0:12	Investigation of a side reaction	0:31	Investigation of a side reaction occurring during N-linked glycan labeling by cationic tags.
30086833	5	35	theme	sugars	964:969	arg1	derivatization					946:959	derivatization	946:959	derivatization of sugars via a hydrazone formation	946:995	Finally, it was confirmed that derivatization of sugars via a hydrazone formation can be a simpler method with a high reaction yield suitable for high sensitive CE-ESI/MS analyses of N-linked glycans.
30086833	5	35	theme	sugars	964:969	arg1	method					1014:1019	a simpler method	1004:1019	a simpler method with a high reaction yield suitable for high sensitive CE-ESI/MS analyses of N-linked glycans	1004:1113	Finally, it was confirmed that derivatization of sugars via a hydrazone formation can be a simpler method with a high reaction yield suitable for high sensitive CE-ESI/MS analyses of N-linked glycans.
30086833	3	36	link	N-linked	683:690	arg1	glycans					692:698	N-linked glycans	683:698	N-linked glycans	683:698	However, a formation of multiple labeled N-linked glycans was observed by CE-MS in a positive ion mode when positively charged labels such as aliphatic amines containing a quaternary ammonium group were attached to N-linked glycans by reductive amination.
30086833	0	37	theme	N-linked	50:57	arg1	labeling					66:73	N-linked glycan labeling	50:73	N-linked glycan labeling by cationic tags	50:90	Investigation of a side reaction occurring during N-linked glycan labeling by cationic tags.
30086833	1	38	with	glycans	199:205	arg1	charge					224:229	an attached charge	212:229	an attached charge label facilitating electrophoretic migration and electrospray ionization efficiency	212:313	Capillary electrophoresis-mass spectrometry was applied for the analysis of oligosaccharides and N-linked glycans with an attached charge label facilitating electrophoretic migration and electrospray ionization efficiency.
30086833	3	39	theme	reductive	703:711	arg1	amination					713:721	reductive amination	703:721	reductive amination	703:721	However, a formation of multiple labeled N-linked glycans was observed by CE-MS in a positive ion mode when positively charged labels such as aliphatic amines containing a quaternary ammonium group were attached to N-linked glycans by reductive amination.
30086833	3	40	theme	quaternary	640:649	arg1	group					660:664	a quaternary ammonium group	638:664	a quaternary ammonium group	638:664	However, a formation of multiple labeled N-linked glycans was observed by CE-MS in a positive ion mode when positively charged labels such as aliphatic amines containing a quaternary ammonium group were attached to N-linked glycans by reductive amination.
30086833	2	41	theme	tagging	395:401	arg1	formation					457:465	hydrazone formation	447:465	hydrazone formation	447:465	Several different labeling strategies have been tested with different tags and tagging reactions including reductive amination and hydrazone formation.
30086833	2	41	theme	tagging	395:401	arg1	reactions					403:411	tagging reactions	395:411	tagging reactions	395:411	Several different labeling strategies have been tested with different tags and tagging reactions including reductive amination and hydrazone formation.
30086833	2	41	theme	tagging	395:401	arg1	amination					433:441	reductive amination	423:441	reductive amination	423:441	Several different labeling strategies have been tested with different tags and tagging reactions including reductive amination and hydrazone formation.
30086833	5	42	theme	high	1028:1031	arg1	yield					1042:1046	a high reaction yield	1026:1046	a high reaction yield suitable for high sensitive CE-ESI/MS analyses of N-linked glycans	1026:1113	Finally, it was confirmed that derivatization of sugars via a hydrazone formation can be a simpler method with a high reaction yield suitable for high sensitive CE-ESI/MS analyses of N-linked glycans.
30086833	3	43	theme	ammonium	651:658	arg1	group					660:664	a quaternary ammonium group	638:664	a quaternary ammonium group	638:664	However, a formation of multiple labeled N-linked glycans was observed by CE-MS in a positive ion mode when positively charged labels such as aliphatic amines containing a quaternary ammonium group were attached to N-linked glycans by reductive amination.
30086833	3	44	theme	positive	553:560	arg1	mode					566:569	a positive ion mode	551:569	a positive ion mode	551:569	However, a formation of multiple labeled N-linked glycans was observed by CE-MS in a positive ion mode when positively charged labels such as aliphatic amines containing a quaternary ammonium group were attached to N-linked glycans by reductive amination.
30086833	3	45	theme	N-linked	683:690	arg1	glycans					692:698	N-linked glycans	683:698	N-linked glycans	683:698	However, a formation of multiple labeled N-linked glycans was observed by CE-MS in a positive ion mode when positively charged labels such as aliphatic amines containing a quaternary ammonium group were attached to N-linked glycans by reductive amination.
30086833	1	46	dep	charge	224:229	arg1	label					231:235	label	231:235	label facilitating electrophoretic migration and electrospray ionization efficiency	231:313	Capillary electrophoresis-mass spectrometry was applied for the analysis of oligosaccharides and N-linked glycans with an attached charge label facilitating electrophoretic migration and electrospray ionization efficiency.
30086833	5	47	theme	glycans	1107:1113	arg1	analyses					1086:1093	high sensitive CE-ESI/MS analyses	1061:1093	high sensitive CE-ESI/MS analyses of N-linked glycans	1061:1113	Finally, it was confirmed that derivatization of sugars via a hydrazone formation can be a simpler method with a high reaction yield suitable for high sensitive CE-ESI/MS analyses of N-linked glycans.
30086833	5	48	theme	simpler	1006:1012	arg1	derivatization					946:959	derivatization	946:959	derivatization of sugars via a hydrazone formation	946:995	Finally, it was confirmed that derivatization of sugars via a hydrazone formation can be a simpler method with a high reaction yield suitable for high sensitive CE-ESI/MS analyses of N-linked glycans.
30086833	5	48	theme	simpler	1006:1012	arg1	method					1014:1019	a simpler method	1004:1019	a simpler method with a high reaction yield suitable for high sensitive CE-ESI/MS analyses of N-linked glycans	1004:1113	Finally, it was confirmed that derivatization of sugars via a hydrazone formation can be a simpler method with a high reaction yield suitable for high sensitive CE-ESI/MS analyses of N-linked glycans.
30086833	3	49	theme	aliphatic	610:618	arg1	amines					620:625	aliphatic amines	610:625	aliphatic amines containing a quaternary ammonium group	610:664	However, a formation of multiple labeled N-linked glycans was observed by CE-MS in a positive ion mode when positively charged labels such as aliphatic amines containing a quaternary ammonium group were attached to N-linked glycans by reductive amination.
30086833	5	50	theme	high	1061:1064	arg1	analyses					1086:1093	high sensitive CE-ESI/MS analyses	1061:1093	high sensitive CE-ESI/MS analyses of N-linked glycans	1061:1113	Finally, it was confirmed that derivatization of sugars via a hydrazone formation can be a simpler method with a high reaction yield suitable for high sensitive CE-ESI/MS analyses of N-linked glycans.
30086833	5	51	theme	CE-ESI/MS	1076:1084	arg1	analyses					1086:1093	high sensitive CE-ESI/MS analyses	1061:1093	high sensitive CE-ESI/MS analyses of N-linked glycans	1061:1113	Finally, it was confirmed that derivatization of sugars via a hydrazone formation can be a simpler method with a high reaction yield suitable for high sensitive CE-ESI/MS analyses of N-linked glycans.
30086833	4	52	theme	product	819:825	arg1	formation					827:835	the multiple product formation	806:835	the multiple product formation	806:835	A reaction mechanism explaining a side reaction occurring during the labeling and the multiple product formation was proposed and confirmed by using isotopically labeled N-acetylglucosamine.
30086833	2	53	theme	different	376:384	arg1	formation					457:465	hydrazone formation	447:465	hydrazone formation	447:465	Several different labeling strategies have been tested with different tags and tagging reactions including reductive amination and hydrazone formation.
30086833	2	53	theme	different	376:384	arg1	tags					386:389	different tags	376:389	different tags	376:389	Several different labeling strategies have been tested with different tags and tagging reactions including reductive amination and hydrazone formation.
30086833	2	53	theme	different	376:384	arg1	amination					433:441	reductive amination	423:441	reductive amination	423:441	Several different labeling strategies have been tested with different tags and tagging reactions including reductive amination and hydrazone formation.
30086833	1	54	theme	electrophoretic	250:264	arg1	migration					266:274	electrophoretic migration	250:274	electrophoretic migration	250:274	Capillary electrophoresis-mass spectrometry was applied for the analysis of oligosaccharides and N-linked glycans with an attached charge label facilitating electrophoretic migration and electrospray ionization efficiency.
30086833	3	55	theme	N-linked	509:516	arg1	glycans					518:524	multiple labeled N-linked glycans	492:524	multiple labeled N-linked glycans	492:524	However, a formation of multiple labeled N-linked glycans was observed by CE-MS in a positive ion mode when positively charged labels such as aliphatic amines containing a quaternary ammonium group were attached to N-linked glycans by reductive amination.
30086833	4	56	theme	labeled	886:892	arg1	N-acetylglucosamine					894:912	isotopically labeled N-acetylglucosamine	873:912	isotopically labeled N-acetylglucosamine	873:912	A reaction mechanism explaining a side reaction occurring during the labeling and the multiple product formation was proposed and confirmed by using isotopically labeled N-acetylglucosamine.
30086833	4	57	theme	side	758:761	arg1	reaction					763:770	a side reaction	756:770	a side reaction occurring during the labeling	756:800	A reaction mechanism explaining a side reaction occurring during the labeling and the multiple product formation was proposed and confirmed by using isotopically labeled N-acetylglucosamine.
30086833	3	58	attach	attached	671:678	arg3	amination					713:721	reductive amination	703:721	reductive amination	703:721	However, a formation of multiple labeled N-linked glycans was observed by CE-MS in a positive ion mode when positively charged labels such as aliphatic amines containing a quaternary ammonium group were attached to N-linked glycans by reductive amination.
30086833	3	58	attach	attached	671:678	arg2	amines					620:625	aliphatic amines	610:625	aliphatic amines containing a quaternary ammonium group	610:664	However, a formation of multiple labeled N-linked glycans was observed by CE-MS in a positive ion mode when positively charged labels such as aliphatic amines containing a quaternary ammonium group were attached to N-linked glycans by reductive amination.
30086833	3	58	attach	attached	671:678	arg2	labels					595:600	positively charged labels	576:600	positively charged labels such as aliphatic amines containing a quaternary ammonium group	576:664	However, a formation of multiple labeled N-linked glycans was observed by CE-MS in a positive ion mode when positively charged labels such as aliphatic amines containing a quaternary ammonium group were attached to N-linked glycans by reductive amination.
30086833	3	58	attach	attached	671:678	arg1	glycans					692:698	N-linked glycans	683:698	N-linked glycans	683:698	However, a formation of multiple labeled N-linked glycans was observed by CE-MS in a positive ion mode when positively charged labels such as aliphatic amines containing a quaternary ammonium group were attached to N-linked glycans by reductive amination.
30086833	0	59	theme	glycan	59:64	arg1	labeling					66:73	N-linked glycan labeling	50:73	N-linked glycan labeling by cationic tags	50:90	Investigation of a side reaction occurring during N-linked glycan labeling by cationic tags.
30086833	2	60	theme	reductive	423:431	arg1	amination					433:441	reductive amination	423:441	reductive amination	423:441	Several different labeling strategies have been tested with different tags and tagging reactions including reductive amination and hydrazone formation.
30552927	1	0	theme	gut	314:316	arg1	microbiota					318:327	human gut microbiota	308:327	human gut microbiota of Lycium barbarum polysaccharides (LBPS)	308:369	In the present study, the digestion under simulated saliva, gastric and small intestinal conditions and in vitro fermentation by human gut microbiota of Lycium barbarum polysaccharides (LBPS) were investigated.
30552927	0	1	theme	barbarum	169:176	arg1	fruits					152:157	the fruits	148:157	the fruits of Lycium barbarum	148:176	In vitro digestion under simulated saliva, gastric and small intestinal conditions and fermentation by human gut microbiota of polysaccharides from the fruits of Lycium barbarum.
30552927	3	2	theme	2.15 mol	658:665	arg1	glucose					649:655	glucose	649:655	glucose (2.15 mol%)	649:667	Furthermore, LBPS was mainly consisted of glucuronic acid (5.12 mol%), galacturonic acid (12.40 mol%), glucose (2.15 mol%), galactose (39.67 mol%) and arabinose (40.66 mol%).
30552927	3	2	theme	2.15 mol	658:665	arg1	%					666:666	2.15 mol%	658:666	2.15 mol%	658:666	Furthermore, LBPS was mainly consisted of glucuronic acid (5.12 mol%), galacturonic acid (12.40 mol%), glucose (2.15 mol%), galactose (39.67 mol%) and arabinose (40.66 mol%).
30552927	0	3	theme	intestinal	61:70	arg1	conditions					72:81	gastric and small intestinal conditions	43:81	gastric and small intestinal conditions	43:81	In vitro digestion under simulated saliva, gastric and small intestinal conditions and fermentation by human gut microbiota of polysaccharides from the fruits of Lycium barbarum.
30552927	3	4	theme	12.40 mol	636:644	arg1	acid					630:633	galacturonic acid	617:633	galacturonic acid (12.40 mol%)	617:646	Furthermore, LBPS was mainly consisted of glucuronic acid (5.12 mol%), galacturonic acid (12.40 mol%), glucose (2.15 mol%), galactose (39.67 mol%) and arabinose (40.66 mol%).
30552927	3	4	theme	12.40 mol	636:644	arg1	%					645:645	12.40 mol%	636:645	12.40 mol%	636:645	Furthermore, LBPS was mainly consisted of glucuronic acid (5.12 mol%), galacturonic acid (12.40 mol%), glucose (2.15 mol%), galactose (39.67 mol%) and arabinose (40.66 mol%).
30552927	0	5	theme	Lycium	162:167	arg1	barbarum					169:176	Lycium barbarum	162:176	Lycium barbarum	162:176	In vitro digestion under simulated saliva, gastric and small intestinal conditions and fermentation by human gut microbiota of polysaccharides from the fruits of Lycium barbarum.
30552927	7	6	theme	microbial	1141:1149	arg1	community					1151:1159	the microbial community	1137:1159	the microbial community	1137:1159	Meanwhile, LBPS could alter the microbial community after 24 h fermentation, especially enhancing the relative abundances of genera Bacteroides, Bifidobacterium, Phascolarctobacterium, Clostridium XlVb, Prevotella and Collinsella.
30552927	0	7	from	fruits	152:157	arg1	polysaccharides					127:141	polysaccharides	127:141	polysaccharides from the fruits of Lycium barbarum	127:176	In vitro digestion under simulated saliva, gastric and small intestinal conditions and fermentation by human gut microbiota of polysaccharides from the fruits of Lycium barbarum.
30552927	0	7	from	fruits	152:157	arg1	microbiota					113:122	human gut microbiota	103:122	human gut microbiota of polysaccharides from the fruits of Lycium barbarum	103:176	In vitro digestion under simulated saliva, gastric and small intestinal conditions and fermentation by human gut microbiota of polysaccharides from the fruits of Lycium barbarum.
30552927	8	8	theme	microbiota	1437:1446	arg1	composition					1448:1458	gut microbiota composition	1433:1458	gut microbiota composition	1433:1458	All the results suggested that LBPS could be developed as special ingredients for modulating gut microbiota composition and promoting the growth of beneficial bacteria like Bifidobacterium.
30552927	5	9	theme	molecular	914:922	arg1	weight					924:929	molecular weight	914:929	molecular weight	914:929	Whereas, LBPS could be utilized by gut microbiota based on the consumption of monosaccharides, decreased molecular weight and lower content of total carbohydrates after fermentation in vitro.
30552927	5	10	theme	gut	844:846	arg1	microbiota					848:857	gut microbiota	844:857	gut microbiota based on the consumption of monosaccharides	844:901	Whereas, LBPS could be utilized by gut microbiota based on the consumption of monosaccharides, decreased molecular weight and lower content of total carbohydrates after fermentation in vitro.
30552927	1	11	theme	gastric	239:245	arg1	conditions					268:277	gastric and small intestinal conditions	239:277	gastric and small intestinal conditions	239:277	In the present study, the digestion under simulated saliva, gastric and small intestinal conditions and in vitro fermentation by human gut microbiota of Lycium barbarum polysaccharides (LBPS) were investigated.
30552927	1	12	theme	Lycium	332:337	arg1	LBPS					365:368	LBPS	365:368	LBPS	365:368	In the present study, the digestion under simulated saliva, gastric and small intestinal conditions and in vitro fermentation by human gut microbiota of Lycium barbarum polysaccharides (LBPS) were investigated.
30552927	1	12	theme	Lycium	332:337	arg1	polysaccharides					348:362	Lycium barbarum polysaccharides	332:362	Lycium barbarum polysaccharides (LBPS)	332:369	In the present study, the digestion under simulated saliva, gastric and small intestinal conditions and in vitro fermentation by human gut microbiota of Lycium barbarum polysaccharides (LBPS) were investigated.
30552927	2	13	contain	contained	408:416	arg2	result					395:400	a result	393:400	a result	393:400	As a result, LBPS contained carbohydrates, uronic acids, protein and polyphenols of 60.96 ± 5.58, 20.98 ± 2.49, 5.30 ± 0.12 and 1.04 ± 0.06%, respectively.
30552927	2	13	contain	contained	408:416	arg2	protein					447:453	protein	447:453	protein	447:453	As a result, LBPS contained carbohydrates, uronic acids, protein and polyphenols of 60.96 ± 5.58, 20.98 ± 2.49, 5.30 ± 0.12 and 1.04 ± 0.06%, respectively.
30552927	2	13	contain	contained	408:416	arg1	LBPS					403:406	LBPS	403:406	LBPS	403:406	As a result, LBPS contained carbohydrates, uronic acids, protein and polyphenols of 60.96 ± 5.58, 20.98 ± 2.49, 5.30 ± 0.12 and 1.04 ± 0.06%, respectively.
30552927	2	13	contain	contained	408:416	arg2	polyphenols					459:469	polyphenols	459:469	polyphenols of 60.96 ± 5.58, 20.98 ± 2.49, 5.30 ± 0.12 and 1.04 ± 0.06%	459:529	As a result, LBPS contained carbohydrates, uronic acids, protein and polyphenols of 60.96 ± 5.58, 20.98 ± 2.49, 5.30 ± 0.12 and 1.04 ± 0.06%, respectively.
30552927	2	13	contain	contained	408:416	arg2	acids					440:444	uronic acids	433:444	uronic acids	433:444	As a result, LBPS contained carbohydrates, uronic acids, protein and polyphenols of 60.96 ± 5.58, 20.98 ± 2.49, 5.30 ± 0.12 and 1.04 ± 0.06%, respectively.
30552927	2	13	contain	contained	408:416	arg2	carbohydrates					418:430	carbohydrates	418:430	carbohydrates	418:430	As a result, LBPS contained carbohydrates, uronic acids, protein and polyphenols of 60.96 ± 5.58, 20.98 ± 2.49, 5.30 ± 0.12 and 1.04 ± 0.06%, respectively.
30552927	0	14	theme	human	103:107	arg1	microbiota					113:122	human gut microbiota	103:122	human gut microbiota of polysaccharides from the fruits of Lycium barbarum	103:176	In vitro digestion under simulated saliva, gastric and small intestinal conditions and fermentation by human gut microbiota of polysaccharides from the fruits of Lycium barbarum.
30552927	1	15	theme	barbarum	339:346	arg1	LBPS					365:368	LBPS	365:368	LBPS	365:368	In the present study, the digestion under simulated saliva, gastric and small intestinal conditions and in vitro fermentation by human gut microbiota of Lycium barbarum polysaccharides (LBPS) were investigated.
30552927	1	15	theme	barbarum	339:346	arg1	polysaccharides					348:362	Lycium barbarum polysaccharides	332:362	Lycium barbarum polysaccharides (LBPS)	332:369	In the present study, the digestion under simulated saliva, gastric and small intestinal conditions and in vitro fermentation by human gut microbiota of Lycium barbarum polysaccharides (LBPS) were investigated.
30552927	4	16	theme	intestinal	763:772	arg1	conditions					774:783	gastric and small intestinal conditions	745:783	gastric and small intestinal conditions	745:783	Under simulated saliva, gastric and small intestinal conditions, LBPS was not affected.
30552927	1	17	theme	polysaccharides	348:362	arg1	microbiota					318:327	human gut microbiota	308:327	human gut microbiota of Lycium barbarum polysaccharides (LBPS)	308:369	In the present study, the digestion under simulated saliva, gastric and small intestinal conditions and in vitro fermentation by human gut microbiota of Lycium barbarum polysaccharides (LBPS) were investigated.
30552927	5	18	used	utilized	832:839	arg2	LBPS					818:821	LBPS	818:821	LBPS	818:821	Whereas, LBPS could be utilized by gut microbiota based on the consumption of monosaccharides, decreased molecular weight and lower content of total carbohydrates after fermentation in vitro.
30552927	0	19	theme	In	0:1	arg1	digestion					9:17	In vitro digestion	0:17	In vitro digestion under simulated saliva, gastric and small intestinal conditions and fermentation by human gut microbiota of polysaccharides from the fruits of Lycium barbarum.	0:177	In vitro digestion under simulated saliva, gastric and small intestinal conditions and fermentation by human gut microbiota of polysaccharides from the fruits of Lycium barbarum.
30552927	8	20	theme	beneficial	1488:1497	arg1	bacteria					1499:1506	beneficial bacteria	1488:1506	beneficial bacteria like Bifidobacterium	1488:1527	All the results suggested that LBPS could be developed as special ingredients for modulating gut microbiota composition and promoting the growth of beneficial bacteria like Bifidobacterium.
30552927	4	21	theme	gastric	745:751	arg1	conditions					774:783	gastric and small intestinal conditions	745:783	gastric and small intestinal conditions	745:783	Under simulated saliva, gastric and small intestinal conditions, LBPS was not affected.
30552927	7	22	theme	Phascolarctobacterium	1271:1291	arg1	abundances					1220:1229	the relative abundances	1207:1229	the relative abundances of genera Bacteroides, Bifidobacterium, Phascolarctobacterium, Clostridium XlVb, Prevotella and Collinsella	1207:1337	Meanwhile, LBPS could alter the microbial community after 24 h fermentation, especially enhancing the relative abundances of genera Bacteroides, Bifidobacterium, Phascolarctobacterium, Clostridium XlVb, Prevotella and Collinsella.
30552927	1	23	theme	intestinal	257:266	arg1	conditions					268:277	gastric and small intestinal conditions	239:277	gastric and small intestinal conditions	239:277	In the present study, the digestion under simulated saliva, gastric and small intestinal conditions and in vitro fermentation by human gut microbiota of Lycium barbarum polysaccharides (LBPS) were investigated.
30552927	2	24	theme	%	529:529	arg1	polyphenols					459:469	polyphenols	459:469	polyphenols of 60.96 ± 5.58, 20.98 ± 2.49, 5.30 ± 0.12 and 1.04 ± 0.06%	459:529	As a result, LBPS contained carbohydrates, uronic acids, protein and polyphenols of 60.96 ± 5.58, 20.98 ± 2.49, 5.30 ± 0.12 and 1.04 ± 0.06%, respectively.
30552927	2	24	theme	%	529:529	arg1	protein					447:453	protein	447:453	protein	447:453	As a result, LBPS contained carbohydrates, uronic acids, protein and polyphenols of 60.96 ± 5.58, 20.98 ± 2.49, 5.30 ± 0.12 and 1.04 ± 0.06%, respectively.
30552927	2	24	theme	%	529:529	arg1	carbohydrates					418:430	carbohydrates	418:430	carbohydrates	418:430	As a result, LBPS contained carbohydrates, uronic acids, protein and polyphenols of 60.96 ± 5.58, 20.98 ± 2.49, 5.30 ± 0.12 and 1.04 ± 0.06%, respectively.
30552927	2	24	theme	%	529:529	arg1	acids					440:444	uronic acids	433:444	uronic acids	433:444	As a result, LBPS contained carbohydrates, uronic acids, protein and polyphenols of 60.96 ± 5.58, 20.98 ± 2.49, 5.30 ± 0.12 and 1.04 ± 0.06%, respectively.
30552927	2	24	theme	%	529:529	arg1	result					395:400	a result	393:400	a result	393:400	As a result, LBPS contained carbohydrates, uronic acids, protein and polyphenols of 60.96 ± 5.58, 20.98 ± 2.49, 5.30 ± 0.12 and 1.04 ± 0.06%, respectively.
30552927	7	25	theme	relative	1211:1218	arg1	abundances					1220:1229	the relative abundances	1207:1229	the relative abundances of genera Bacteroides, Bifidobacterium, Phascolarctobacterium, Clostridium XlVb, Prevotella and Collinsella	1207:1337	Meanwhile, LBPS could alter the microbial community after 24 h fermentation, especially enhancing the relative abundances of genera Bacteroides, Bifidobacterium, Phascolarctobacterium, Clostridium XlVb, Prevotella and Collinsella.
30552927	4	26	dep	saliva	737:742	arg1	conditions					774:783	gastric and small intestinal conditions	745:783	gastric and small intestinal conditions	745:783	Under simulated saliva, gastric and small intestinal conditions, LBPS was not affected.
30552927	0	27	theme	gut	109:111	arg1	microbiota					113:122	human gut microbiota	103:122	human gut microbiota of polysaccharides from the fruits of Lycium barbarum	103:176	In vitro digestion under simulated saliva, gastric and small intestinal conditions and fermentation by human gut microbiota of polysaccharides from the fruits of Lycium barbarum.
30552927	5	28	theme	total	952:956	arg1	carbohydrates					958:970	total carbohydrates	952:970	total carbohydrates	952:970	Whereas, LBPS could be utilized by gut microbiota based on the consumption of monosaccharides, decreased molecular weight and lower content of total carbohydrates after fermentation in vitro.
30552927	7	29	theme	Collinsella	1327:1337	arg1	abundances					1220:1229	the relative abundances	1207:1229	the relative abundances of genera Bacteroides, Bifidobacterium, Phascolarctobacterium, Clostridium XlVb, Prevotella and Collinsella	1207:1337	Meanwhile, LBPS could alter the microbial community after 24 h fermentation, especially enhancing the relative abundances of genera Bacteroides, Bifidobacterium, Phascolarctobacterium, Clostridium XlVb, Prevotella and Collinsella.
30552927	1	30	theme	present	186:192	arg1	study					194:198	the present study	182:198	the present study	182:198	In the present study, the digestion under simulated saliva, gastric and small intestinal conditions and in vitro fermentation by human gut microbiota of Lycium barbarum polysaccharides (LBPS) were investigated.
30552927	8	31	theme	bacteria	1499:1506	arg1	growth					1478:1483	the growth	1474:1483	the growth of beneficial bacteria like Bifidobacterium	1474:1527	All the results suggested that LBPS could be developed as special ingredients for modulating gut microbiota composition and promoting the growth of beneficial bacteria like Bifidobacterium.
30552927	0	32	theme	polysaccharides	127:141	arg1	microbiota					113:122	human gut microbiota	103:122	human gut microbiota of polysaccharides from the fruits of Lycium barbarum	103:176	In vitro digestion under simulated saliva, gastric and small intestinal conditions and fermentation by human gut microbiota of polysaccharides from the fruits of Lycium barbarum.
30552927	5	33	theme	carbohydrates	958:970	arg1	content					941:947	lower content	935:947	lower content of total carbohydrates	935:970	Whereas, LBPS could be utilized by gut microbiota based on the consumption of monosaccharides, decreased molecular weight and lower content of total carbohydrates after fermentation in vitro.
30552927	5	33	theme	carbohydrates	958:970	arg1	weight					924:929	molecular weight	914:929	molecular weight	914:929	Whereas, LBPS could be utilized by gut microbiota based on the consumption of monosaccharides, decreased molecular weight and lower content of total carbohydrates after fermentation in vitro.
30552927	6	34	theme	fatty	1096:1100	arg1	acids					1102:1106	short-chain fatty acids	1084:1106	short-chain fatty acids	1084:1106	With the proceeding of fermentation, LBPS significantly promoted the production of short-chain fatty acids.
30552927	7	35	dep	Phascolarctobacterium	1271:1291	arg1	Bifidobacterium					1254:1268	genera Bacteroides, Bifidobacterium, Phascolarctobacterium	1234:1291	Bifidobacterium	1254:1268	Meanwhile, LBPS could alter the microbial community after 24 h fermentation, especially enhancing the relative abundances of genera Bacteroides, Bifidobacterium, Phascolarctobacterium, Clostridium XlVb, Prevotella and Collinsella.
30552927	0	36	theme	simulated	25:33	arg1	saliva					35:40	simulated saliva	25:40	simulated saliva	25:40	In vitro digestion under simulated saliva, gastric and small intestinal conditions and fermentation by human gut microbiota of polysaccharides from the fruits of Lycium barbarum.
30552927	1	37	theme	in	283:284	arg1	fermentation					292:303	in vitro fermentation	283:303	in vitro fermentation by human gut microbiota of Lycium barbarum polysaccharides (LBPS)	283:369	In the present study, the digestion under simulated saliva, gastric and small intestinal conditions and in vitro fermentation by human gut microbiota of Lycium barbarum polysaccharides (LBPS) were investigated.
30552927	7	38	theme	Prevotella	1312:1321	arg1	abundances					1220:1229	the relative abundances	1207:1229	the relative abundances of genera Bacteroides, Bifidobacterium, Phascolarctobacterium, Clostridium XlVb, Prevotella and Collinsella	1207:1337	Meanwhile, LBPS could alter the microbial community after 24 h fermentation, especially enhancing the relative abundances of genera Bacteroides, Bifidobacterium, Phascolarctobacterium, Clostridium XlVb, Prevotella and Collinsella.
30552927	0	39	theme	gastric	43:49	arg1	conditions					72:81	gastric and small intestinal conditions	43:81	gastric and small intestinal conditions	43:81	In vitro digestion under simulated saliva, gastric and small intestinal conditions and fermentation by human gut microbiota of polysaccharides from the fruits of Lycium barbarum.
30552927	3	40	theme	5.12 mol	605:612	arg1	%					613:613	5.12 mol%	605:613	5.12 mol%	605:613	Furthermore, LBPS was mainly consisted of glucuronic acid (5.12 mol%), galacturonic acid (12.40 mol%), glucose (2.15 mol%), galactose (39.67 mol%) and arabinose (40.66 mol%).
30552927	3	40	theme	5.12 mol	605:612	arg1	acid					599:602	glucuronic acid	588:602	glucuronic acid (5.12 mol%)	588:614	Furthermore, LBPS was mainly consisted of glucuronic acid (5.12 mol%), galacturonic acid (12.40 mol%), glucose (2.15 mol%), galactose (39.67 mol%) and arabinose (40.66 mol%).
30552927	4	41	theme	simulated	727:735	arg1	saliva					737:742	simulated saliva	727:742	simulated saliva	727:742	Under simulated saliva, gastric and small intestinal conditions, LBPS was not affected.
30552927	1	42	dep	in	283:284	arg1	vitro					286:290	vitro	286:290	vitro	286:290	In the present study, the digestion under simulated saliva, gastric and small intestinal conditions and in vitro fermentation by human gut microbiota of Lycium barbarum polysaccharides (LBPS) were investigated.
30552927	6	43	theme	fermentation	1024:1035	arg1	proceeding					1010:1019	the proceeding	1006:1019	the proceeding of fermentation	1006:1035	With the proceeding of fermentation, LBPS significantly promoted the production of short-chain fatty acids.
30552927	3	44	theme	39.67 mol	681:689	arg1	galactose					670:678	galactose	670:678	galactose (39.67 mol%)	670:691	Furthermore, LBPS was mainly consisted of glucuronic acid (5.12 mol%), galacturonic acid (12.40 mol%), glucose (2.15 mol%), galactose (39.67 mol%) and arabinose (40.66 mol%).
30552927	3	44	theme	39.67 mol	681:689	arg1	%					690:690	39.67 mol%	681:690	39.67 mol%	681:690	Furthermore, LBPS was mainly consisted of glucuronic acid (5.12 mol%), galacturonic acid (12.40 mol%), glucose (2.15 mol%), galactose (39.67 mol%) and arabinose (40.66 mol%).
30552927	8	45	theme	gut	1433:1435	arg1	composition					1448:1458	gut microbiota composition	1433:1458	gut microbiota composition	1433:1458	All the results suggested that LBPS could be developed as special ingredients for modulating gut microbiota composition and promoting the growth of beneficial bacteria like Bifidobacterium.
30552927	8	46	theme	special	1398:1404	arg1	LBPS					1371:1374	LBPS	1371:1374	LBPS	1371:1374	All the results suggested that LBPS could be developed as special ingredients for modulating gut microbiota composition and promoting the growth of beneficial bacteria like Bifidobacterium.
30552927	8	46	theme	special	1398:1404	arg1	ingredients					1406:1416	special ingredients	1398:1416	special ingredients for modulating gut microbiota composition and promoting the growth of beneficial bacteria like Bifidobacterium	1398:1527	All the results suggested that LBPS could be developed as special ingredients for modulating gut microbiota composition and promoting the growth of beneficial bacteria like Bifidobacterium.
30552927	5	47	theme	monosaccharides	887:901	arg1	consumption					872:882	the consumption	868:882	the consumption of monosaccharides	868:901	Whereas, LBPS could be utilized by gut microbiota based on the consumption of monosaccharides, decreased molecular weight and lower content of total carbohydrates after fermentation in vitro.
30552927	5	48	theme	lower	935:939	arg1	content					941:947	lower content	935:947	lower content of total carbohydrates	935:970	Whereas, LBPS could be utilized by gut microbiota based on the consumption of monosaccharides, decreased molecular weight and lower content of total carbohydrates after fermentation in vitro.
30552927	3	49	theme	glucuronic	588:597	arg1	%					613:613	5.12 mol%	605:613	5.12 mol%	605:613	Furthermore, LBPS was mainly consisted of glucuronic acid (5.12 mol%), galacturonic acid (12.40 mol%), glucose (2.15 mol%), galactose (39.67 mol%) and arabinose (40.66 mol%).
30552927	3	49	theme	glucuronic	588:597	arg1	acid					599:602	glucuronic acid	588:602	glucuronic acid (5.12 mol%)	588:614	Furthermore, LBPS was mainly consisted of glucuronic acid (5.12 mol%), galacturonic acid (12.40 mol%), glucose (2.15 mol%), galactose (39.67 mol%) and arabinose (40.66 mol%).
30552927	7	50	theme	24 h	1167:1170	arg1	fermentation					1172:1183	24 h fermentation	1167:1183	24 h fermentation	1167:1183	Meanwhile, LBPS could alter the microbial community after 24 h fermentation, especially enhancing the relative abundances of genera Bacteroides, Bifidobacterium, Phascolarctobacterium, Clostridium XlVb, Prevotella and Collinsella.
30552927	3	51	theme	40.66 mol	708:716	arg1	arabinose					697:705	arabinose	697:705	arabinose (40.66 mol%)	697:718	Furthermore, LBPS was mainly consisted of glucuronic acid (5.12 mol%), galacturonic acid (12.40 mol%), glucose (2.15 mol%), galactose (39.67 mol%) and arabinose (40.66 mol%).
30552927	3	51	theme	40.66 mol	708:716	arg1	%					717:717	40.66 mol%	708:717	40.66 mol%	708:717	Furthermore, LBPS was mainly consisted of glucuronic acid (5.12 mol%), galacturonic acid (12.40 mol%), glucose (2.15 mol%), galactose (39.67 mol%) and arabinose (40.66 mol%).
30552927	6	52	theme	short-chain	1084:1094	arg1	acids					1102:1106	short-chain fatty acids	1084:1106	short-chain fatty acids	1084:1106	With the proceeding of fermentation, LBPS significantly promoted the production of short-chain fatty acids.
30552927	3	53	theme	galacturonic	617:628	arg1	acid					630:633	galacturonic acid	617:633	galacturonic acid (12.40 mol%)	617:646	Furthermore, LBPS was mainly consisted of glucuronic acid (5.12 mol%), galacturonic acid (12.40 mol%), glucose (2.15 mol%), galactose (39.67 mol%) and arabinose (40.66 mol%).
30552927	3	53	theme	galacturonic	617:628	arg1	%					645:645	12.40 mol%	636:645	12.40 mol%	636:645	Furthermore, LBPS was mainly consisted of glucuronic acid (5.12 mol%), galacturonic acid (12.40 mol%), glucose (2.15 mol%), galactose (39.67 mol%) and arabinose (40.66 mol%).
30552927	6	54	theme	acids	1102:1106	arg1	production					1070:1079	the production	1066:1079	the production of short-chain fatty acids	1066:1106	With the proceeding of fermentation, LBPS significantly promoted the production of short-chain fatty acids.
30552927	0	55	from	microbiota	113:122	arg1	fruits					152:157	the fruits	148:157	the fruits of Lycium barbarum	148:176	In vitro digestion under simulated saliva, gastric and small intestinal conditions and fermentation by human gut microbiota of polysaccharides from the fruits of Lycium barbarum.
30552927	2	56	theme	uronic	433:438	arg1	polyphenols					459:469	polyphenols	459:469	polyphenols of 60.96 ± 5.58, 20.98 ± 2.49, 5.30 ± 0.12 and 1.04 ± 0.06%	459:529	As a result, LBPS contained carbohydrates, uronic acids, protein and polyphenols of 60.96 ± 5.58, 20.98 ± 2.49, 5.30 ± 0.12 and 1.04 ± 0.06%, respectively.
30552927	2	56	theme	uronic	433:438	arg1	protein					447:453	protein	447:453	protein	447:453	As a result, LBPS contained carbohydrates, uronic acids, protein and polyphenols of 60.96 ± 5.58, 20.98 ± 2.49, 5.30 ± 0.12 and 1.04 ± 0.06%, respectively.
30552927	2	56	theme	uronic	433:438	arg1	carbohydrates					418:430	carbohydrates	418:430	carbohydrates	418:430	As a result, LBPS contained carbohydrates, uronic acids, protein and polyphenols of 60.96 ± 5.58, 20.98 ± 2.49, 5.30 ± 0.12 and 1.04 ± 0.06%, respectively.
30552927	2	56	theme	uronic	433:438	arg1	acids					440:444	uronic acids	433:444	uronic acids	433:444	As a result, LBPS contained carbohydrates, uronic acids, protein and polyphenols of 60.96 ± 5.58, 20.98 ± 2.49, 5.30 ± 0.12 and 1.04 ± 0.06%, respectively.
30552927	2	56	theme	uronic	433:438	arg1	result					395:400	a result	393:400	a result	393:400	As a result, LBPS contained carbohydrates, uronic acids, protein and polyphenols of 60.96 ± 5.58, 20.98 ± 2.49, 5.30 ± 0.12 and 1.04 ± 0.06%, respectively.
30552927	1	57	theme	human	308:312	arg1	microbiota					318:327	human gut microbiota	308:327	human gut microbiota of Lycium barbarum polysaccharides (LBPS)	308:369	In the present study, the digestion under simulated saliva, gastric and small intestinal conditions and in vitro fermentation by human gut microbiota of Lycium barbarum polysaccharides (LBPS) were investigated.
30552927	0	58	dep	In	0:1	arg1	vitro					3:7	vitro	3:7	vitro	3:7	In vitro digestion under simulated saliva, gastric and small intestinal conditions and fermentation by human gut microbiota of polysaccharides from the fruits of Lycium barbarum.
30552927	1	59	theme	simulated	221:229	arg1	saliva					231:236	simulated saliva	221:236	simulated saliva	221:236	In the present study, the digestion under simulated saliva, gastric and small intestinal conditions and in vitro fermentation by human gut microbiota of Lycium barbarum polysaccharides (LBPS) were investigated.
30036752	0	0	theme	aerobic	86:92	arg1	reactor					136:142	an aerobic bacterial granular photo-sequencing batch reactor	83:142	an aerobic bacterial granular photo-sequencing batch reactor	83:142	Natural sunlight induced rapid formation of water-born algal-bacterial granules in an aerobic bacterial granular photo-sequencing batch reactor.
30036752	6	1	dep	richness	1137:1144	arg1	the					1133:1135	the	1133:1135	the	1133:1135	Illumina results revealed that the introduction of algae reduced the richness and diversity of bacterial community.
30036752	2	2	theme	sunlight	383:390	arg1	exposure					392:399	natural sunlight exposure	375:399	natural sunlight exposure	375:399	Rapid granulation of algal-bacterial granules was achieved in an aerobic bacterial granular sequencing batch reactor (SBR) under natural sunlight exposure.
30036752	8	3	theme	granules	1447:1454	arg1	granulation					1416:1426	granulation	1416:1426	granulation of algal-bacterial granules	1416:1454	The possible mechanism for natural sunlight induced granulation of algal-bacterial granules was thus proposed based on the interactions between algae and bacteria.
30036752	1	4	theme	algal-bacterial	178:192	arg1	granules					194:201	algal-bacterial granules	178:201	algal-bacterial granules	178:201	Wastewater treatment by means of algal-bacterial granules has become a hot topic worldwide recently.
30036752	0	5	theme	granular	104:111	arg1	reactor					136:142	an aerobic bacterial granular photo-sequencing batch reactor	83:142	an aerobic bacterial granular photo-sequencing batch reactor	83:142	Natural sunlight induced rapid formation of water-born algal-bacterial granules in an aerobic bacterial granular photo-sequencing batch reactor.
30036752	3	6	theme	inner	698:702	arg1	side					704:707	the inner side	694:707	the inner side of the granules	694:723	Occurrence of abundant filamentous bacteria bridging the water-born algae, and overproduction of extracellular polymeric substances (EPS) (especially polysaccharides (PS), tryptophan & protein-like, and humic acid-like substances) were observed on the first 3 days, while the algae grew into the inner side of the granules and mature granules were obtained on day 7.
30036752	3	7	theme	acid-like	611:619	arg1	substances					621:630	humic acid-like substances	605:630	humic acid-like substances	605:630	Occurrence of abundant filamentous bacteria bridging the water-born algae, and overproduction of extracellular polymeric substances (EPS) (especially polysaccharides (PS), tryptophan & protein-like, and humic acid-like substances) were observed on the first 3 days, while the algae grew into the inner side of the granules and mature granules were obtained on day 7.
30036752	2	8	theme	bacterial	319:327	arg1	SBR					364:366	SBR	364:366	SBR	364:366	Rapid granulation of algal-bacterial granules was achieved in an aerobic bacterial granular sequencing batch reactor (SBR) under natural sunlight exposure.
30036752	2	8	theme	bacterial	319:327	arg1	reactor					355:361	an aerobic bacterial granular sequencing batch reactor	308:361	an aerobic bacterial granular sequencing batch reactor (SBR)	308:367	Rapid granulation of algal-bacterial granules was achieved in an aerobic bacterial granular sequencing batch reactor (SBR) under natural sunlight exposure.
30036752	2	9	theme	granular	329:336	arg1	SBR					364:366	SBR	364:366	SBR	364:366	Rapid granulation of algal-bacterial granules was achieved in an aerobic bacterial granular sequencing batch reactor (SBR) under natural sunlight exposure.
30036752	2	9	theme	granular	329:336	arg1	reactor					355:361	an aerobic bacterial granular sequencing batch reactor	308:361	an aerobic bacterial granular sequencing batch reactor (SBR)	308:367	Rapid granulation of algal-bacterial granules was achieved in an aerobic bacterial granular sequencing batch reactor (SBR) under natural sunlight exposure.
30036752	3	10	theme	bacteria	437:444	arg1	Occurrence					402:411	Occurrence	402:411	Occurrence of abundant filamentous bacteria bridging the water-born algae	402:474	Occurrence of abundant filamentous bacteria bridging the water-born algae, and overproduction of extracellular polymeric substances (EPS) (especially polysaccharides (PS), tryptophan & protein-like, and humic acid-like substances) were observed on the first 3 days, while the algae grew into the inner side of the granules and mature granules were obtained on day 7.
30036752	3	10	theme	bacteria	437:444	arg1	overproduction					481:494	overproduction	481:494	overproduction of extracellular polymeric substances (EPS) (especially polysaccharides (PS), tryptophan & protein-like, and humic acid-like substances)	481:631	Occurrence of abundant filamentous bacteria bridging the water-born algae, and overproduction of extracellular polymeric substances (EPS) (especially polysaccharides (PS), tryptophan & protein-like, and humic acid-like substances) were observed on the first 3 days, while the algae grew into the inner side of the granules and mature granules were obtained on day 7.
30036752	0	11	theme	bacterial	94:102	arg1	reactor					136:142	an aerobic bacterial granular photo-sequencing batch reactor	83:142	an aerobic bacterial granular photo-sequencing batch reactor	83:142	Natural sunlight induced rapid formation of water-born algal-bacterial granules in an aerobic bacterial granular photo-sequencing batch reactor.
30036752	3	12	theme	&	585:585	arg1	protein-like					587:598	tryptophan & protein-like	574:598	tryptophan & protein-like	574:598	Occurrence of abundant filamentous bacteria bridging the water-born algae, and overproduction of extracellular polymeric substances (EPS) (especially polysaccharides (PS), tryptophan & protein-like, and humic acid-like substances) were observed on the first 3 days, while the algae grew into the inner side of the granules and mature granules were obtained on day 7.
30036752	2	13	theme	aerobic	311:317	arg1	SBR					364:366	SBR	364:366	SBR	364:366	Rapid granulation of algal-bacterial granules was achieved in an aerobic bacterial granular sequencing batch reactor (SBR) under natural sunlight exposure.
30036752	2	13	theme	aerobic	311:317	arg1	reactor					355:361	an aerobic bacterial granular sequencing batch reactor	308:361	an aerobic bacterial granular sequencing batch reactor (SBR)	308:367	Rapid granulation of algal-bacterial granules was achieved in an aerobic bacterial granular sequencing batch reactor (SBR) under natural sunlight exposure.
30036752	7	14	theme	predominant	1294:1304	arg1	algae					1306:1310	the predominant algae	1290:1310	the predominant algae	1290:1310	Besides, few changes in structure and some compositions shifts in bacterial communities were found, while the predominant algae shifted from Diatomea to green algae Chlorophyceae.
30036752	0	15	theme	batch	130:134	arg1	reactor					136:142	an aerobic bacterial granular photo-sequencing batch reactor	83:142	an aerobic bacterial granular photo-sequencing batch reactor	83:142	Natural sunlight induced rapid formation of water-born algal-bacterial granules in an aerobic bacterial granular photo-sequencing batch reactor.
30036752	3	16	theme	mature	729:734	arg1	granules					736:743	mature granules	729:743	mature granules	729:743	Occurrence of abundant filamentous bacteria bridging the water-born algae, and overproduction of extracellular polymeric substances (EPS) (especially polysaccharides (PS), tryptophan & protein-like, and humic acid-like substances) were observed on the first 3 days, while the algae grew into the inner side of the granules and mature granules were obtained on day 7.
30036752	3	17	theme	substances	523:532	arg1	Occurrence					402:411	Occurrence	402:411	Occurrence of abundant filamentous bacteria bridging the water-born algae	402:474	Occurrence of abundant filamentous bacteria bridging the water-born algae, and overproduction of extracellular polymeric substances (EPS) (especially polysaccharides (PS), tryptophan & protein-like, and humic acid-like substances) were observed on the first 3 days, while the algae grew into the inner side of the granules and mature granules were obtained on day 7.
30036752	3	17	theme	substances	523:532	arg1	overproduction					481:494	overproduction	481:494	overproduction of extracellular polymeric substances (EPS) (especially polysaccharides (PS), tryptophan & protein-like, and humic acid-like substances)	481:631	Occurrence of abundant filamentous bacteria bridging the water-born algae, and overproduction of extracellular polymeric substances (EPS) (especially polysaccharides (PS), tryptophan & protein-like, and humic acid-like substances) were observed on the first 3 days, while the algae grew into the inner side of the granules and mature granules were obtained on day 7.
30036752	6	18	theme	bacterial	1163:1171	arg1	community					1173:1181	bacterial community	1163:1181	bacterial community	1163:1181	Illumina results revealed that the introduction of algae reduced the richness and diversity of bacterial community.
30036752	7	19	theme	green	1337:1341	arg1	algae					1343:1347	green algae	1337:1347	green algae Chlorophyceae	1337:1361	Besides, few changes in structure and some compositions shifts in bacterial communities were found, while the predominant algae shifted from Diatomea to green algae Chlorophyceae.
30036752	6	20	theme	Illumina	1068:1075	arg1	results					1077:1083	Illumina results	1068:1083	Illumina results	1068:1083	Illumina results revealed that the introduction of algae reduced the richness and diversity of bacterial community.
30036752	0	21	theme	photo-sequencing	113:128	arg1	reactor					136:142	an aerobic bacterial granular photo-sequencing batch reactor	83:142	an aerobic bacterial granular photo-sequencing batch reactor	83:142	Natural sunlight induced rapid formation of water-born algal-bacterial granules in an aerobic bacterial granular photo-sequencing batch reactor.
30036752	4	22	theme	settleability	827:839	arg1	sizes					847:851	the settleability, mean sizes	823:851	the settleability, mean sizes of the granules	823:867	The growth of the water-born algae slightly decreased the settleability, mean sizes of the granules, but stimulated the bioactivity significantly.
30036752	3	23	theme	filamentous	425:435	arg1	bacteria					437:444	abundant filamentous bacteria	416:444	abundant filamentous bacteria bridging the water-born algae	416:474	Occurrence of abundant filamentous bacteria bridging the water-born algae, and overproduction of extracellular polymeric substances (EPS) (especially polysaccharides (PS), tryptophan & protein-like, and humic acid-like substances) were observed on the first 3 days, while the algae grew into the inner side of the granules and mature granules were obtained on day 7.
30036752	3	24	theme	abundant	416:423	arg1	bacteria					437:444	abundant filamentous bacteria	416:444	abundant filamentous bacteria bridging the water-born algae	416:474	Occurrence of abundant filamentous bacteria bridging the water-born algae, and overproduction of extracellular polymeric substances (EPS) (especially polysaccharides (PS), tryptophan & protein-like, and humic acid-like substances) were observed on the first 3 days, while the algae grew into the inner side of the granules and mature granules were obtained on day 7.
30036752	3	25	theme	granules	716:723	arg1	side					704:707	the inner side	694:707	the inner side of the granules	694:723	Occurrence of abundant filamentous bacteria bridging the water-born algae, and overproduction of extracellular polymeric substances (EPS) (especially polysaccharides (PS), tryptophan & protein-like, and humic acid-like substances) were observed on the first 3 days, while the algae grew into the inner side of the granules and mature granules were obtained on day 7.
30036752	8	26	theme	natural	1391:1397	arg1	sunlight					1399:1406	natural sunlight	1391:1406	natural sunlight induced granulation of algal-bacterial granules	1391:1454	The possible mechanism for natural sunlight induced granulation of algal-bacterial granules was thus proposed based on the interactions between algae and bacteria.
30036752	4	27	dep	settleability	827:839	arg1	mean					842:845	mean	842:845	mean	842:845	The growth of the water-born algae slightly decreased the settleability, mean sizes of the granules, but stimulated the bioactivity significantly.
30036752	8	28	theme	possible	1368:1375	arg1	mechanism					1377:1385	The possible mechanism	1364:1385	The possible mechanism for natural sunlight induced granulation of algal-bacterial granules	1364:1454	The possible mechanism for natural sunlight induced granulation of algal-bacterial granules was thus proposed based on the interactions between algae and bacteria.
30036752	0	29	theme	Natural	0:6	arg1	sunlight					8:15	Natural sunlight	0:15	Natural sunlight	0:15	Natural sunlight induced rapid formation of water-born algal-bacterial granules in an aerobic bacterial granular photo-sequencing batch reactor.
30036752	2	30	theme	batch	349:353	arg1	SBR					364:366	SBR	364:366	SBR	364:366	Rapid granulation of algal-bacterial granules was achieved in an aerobic bacterial granular sequencing batch reactor (SBR) under natural sunlight exposure.
30036752	2	30	theme	batch	349:353	arg1	reactor					355:361	an aerobic bacterial granular sequencing batch reactor	308:361	an aerobic bacterial granular sequencing batch reactor (SBR)	308:367	Rapid granulation of algal-bacterial granules was achieved in an aerobic bacterial granular sequencing batch reactor (SBR) under natural sunlight exposure.
30036752	0	31	theme	rapid	25:29	arg1	formation					31:39	rapid formation	25:39	rapid formation of water-born algal-bacterial granules	25:78	Natural sunlight induced rapid formation of water-born algal-bacterial granules in an aerobic bacterial granular photo-sequencing batch reactor.
30036752	4	32	theme	water-born	787:796	arg1	algae					798:802	the water-born algae	783:802	the water-born algae	783:802	The growth of the water-born algae slightly decreased the settleability, mean sizes of the granules, but stimulated the bioactivity significantly.
30036752	5	33	theme	stable	979:984	arg1	got					975:977	got stable	975:984	got stable	975:984	Whereas, the biomass retention decreased before day 3, and got stable soon with the maturation period with symbiotic growth of algal-bacterial biomass.
30036752	7	34	theme	algae	1343:1347	arg1	Chlorophyceae					1349:1361	green algae Chlorophyceae	1337:1361	green algae Chlorophyceae	1337:1361	Besides, few changes in structure and some compositions shifts in bacterial communities were found, while the predominant algae shifted from Diatomea to green algae Chlorophyceae.
30036752	2	35	theme	sequencing	338:347	arg1	SBR					364:366	SBR	364:366	SBR	364:366	Rapid granulation of algal-bacterial granules was achieved in an aerobic bacterial granular sequencing batch reactor (SBR) under natural sunlight exposure.
30036752	2	35	theme	sequencing	338:347	arg1	reactor					355:361	an aerobic bacterial granular sequencing batch reactor	308:361	an aerobic bacterial granular sequencing batch reactor (SBR)	308:367	Rapid granulation of algal-bacterial granules was achieved in an aerobic bacterial granular sequencing batch reactor (SBR) under natural sunlight exposure.
30036752	1	36	theme	hot	216:218	arg1	worldwide					226:234	a hot topic worldwide	214:234	a hot topic worldwide	214:234	Wastewater treatment by means of algal-bacterial granules has become a hot topic worldwide recently.
30036752	3	37	theme	humic	605:609	arg1	substances					621:630	humic acid-like substances	605:630	humic acid-like substances	605:630	Occurrence of abundant filamentous bacteria bridging the water-born algae, and overproduction of extracellular polymeric substances (EPS) (especially polysaccharides (PS), tryptophan & protein-like, and humic acid-like substances) were observed on the first 3 days, while the algae grew into the inner side of the granules and mature granules were obtained on day 7.
30036752	5	38	theme	biomass	929:935	arg1	retention					937:945	the biomass retention	925:945	the biomass retention	925:945	Whereas, the biomass retention decreased before day 3, and got stable soon with the maturation period with symbiotic growth of algal-bacterial biomass.
30036752	5	39	theme	algal-bacterial	1043:1057	arg1	biomass					1059:1065	algal-bacterial biomass	1043:1065	algal-bacterial biomass	1043:1065	Whereas, the biomass retention decreased before day 3, and got stable soon with the maturation period with symbiotic growth of algal-bacterial biomass.
30036752	2	40	theme	natural	375:381	arg1	exposure					392:399	natural sunlight exposure	375:399	natural sunlight exposure	375:399	Rapid granulation of algal-bacterial granules was achieved in an aerobic bacterial granular sequencing batch reactor (SBR) under natural sunlight exposure.
30036752	5	41	theme	maturation	1000:1009	arg1	period					1011:1016	the maturation period	996:1016	the maturation period	996:1016	Whereas, the biomass retention decreased before day 3, and got stable soon with the maturation period with symbiotic growth of algal-bacterial biomass.
30036752	1	42	theme	topic	220:224	arg1	worldwide					226:234	a hot topic worldwide	214:234	a hot topic worldwide	214:234	Wastewater treatment by means of algal-bacterial granules has become a hot topic worldwide recently.
30036752	5	43	theme	biomass	1059:1065	arg1	growth					1033:1038	symbiotic growth	1023:1038	symbiotic growth of algal-bacterial biomass	1023:1065	Whereas, the biomass retention decreased before day 3, and got stable soon with the maturation period with symbiotic growth of algal-bacterial biomass.
30036752	3	44	theme	water-born	459:468	arg1	algae					470:474	the water-born algae	455:474	the water-born algae	455:474	Occurrence of abundant filamentous bacteria bridging the water-born algae, and overproduction of extracellular polymeric substances (EPS) (especially polysaccharides (PS), tryptophan & protein-like, and humic acid-like substances) were observed on the first 3 days, while the algae grew into the inner side of the granules and mature granules were obtained on day 7.
30036752	3	45	theme	first	654:658	arg1	days					662:665	the first 3 days	650:665	the first 3 days	650:665	Occurrence of abundant filamentous bacteria bridging the water-born algae, and overproduction of extracellular polymeric substances (EPS) (especially polysaccharides (PS), tryptophan & protein-like, and humic acid-like substances) were observed on the first 3 days, while the algae grew into the inner side of the granules and mature granules were obtained on day 7.
30036752	4	46	theme	algae	798:802	arg1	growth					773:778	The growth	769:778	The growth of the water-born algae	769:802	The growth of the water-born algae slightly decreased the settleability, mean sizes of the granules, but stimulated the bioactivity significantly.
30036752	3	47	theme	extracellular	499:511	arg1	EPS					535:537	EPS	535:537	EPS	535:537	Occurrence of abundant filamentous bacteria bridging the water-born algae, and overproduction of extracellular polymeric substances (EPS) (especially polysaccharides (PS), tryptophan & protein-like, and humic acid-like substances) were observed on the first 3 days, while the algae grew into the inner side of the granules and mature granules were obtained on day 7.
30036752	3	47	theme	extracellular	499:511	arg1	substances					523:532	extracellular polymeric substances	499:532	extracellular polymeric substances (EPS) (especially polysaccharides (PS), tryptophan & protein-like, and humic acid-like substances)	499:631	Occurrence of abundant filamentous bacteria bridging the water-born algae, and overproduction of extracellular polymeric substances (EPS) (especially polysaccharides (PS), tryptophan & protein-like, and humic acid-like substances) were observed on the first 3 days, while the algae grew into the inner side of the granules and mature granules were obtained on day 7.
30036752	0	48	theme	algal-bacterial	55:69	arg1	granules					71:78	water-born algal-bacterial granules	44:78	water-born algal-bacterial granules	44:78	Natural sunlight induced rapid formation of water-born algal-bacterial granules in an aerobic bacterial granular photo-sequencing batch reactor.
30036752	7	49	theme	few	1193:1195	arg1	changes					1197:1203	few changes	1193:1203	few changes in structure and some compositions shifts in bacterial communities	1193:1270	Besides, few changes in structure and some compositions shifts in bacterial communities were found, while the predominant algae shifted from Diatomea to green algae Chlorophyceae.
30036752	7	50	from	changes	1197:1203	arg1	structure					1208:1216	structure	1208:1216	structure	1208:1216	Besides, few changes in structure and some compositions shifts in bacterial communities were found, while the predominant algae shifted from Diatomea to green algae Chlorophyceae.
30036752	7	50	from	changes	1197:1203	arg1	shifts					1240:1245	some compositions shifts	1222:1245	some compositions shifts in bacterial communities	1222:1270	Besides, few changes in structure and some compositions shifts in bacterial communities were found, while the predominant algae shifted from Diatomea to green algae Chlorophyceae.
30036752	2	51	theme	Rapid	246:250	arg1	granulation					252:262	Rapid granulation	246:262	Rapid granulation of algal-bacterial granules	246:290	Rapid granulation of algal-bacterial granules was achieved in an aerobic bacterial granular sequencing batch reactor (SBR) under natural sunlight exposure.
30036752	0	52	theme	water-born	44:53	arg1	granules					71:78	water-born algal-bacterial granules	44:78	water-born algal-bacterial granules	44:78	Natural sunlight induced rapid formation of water-born algal-bacterial granules in an aerobic bacterial granular photo-sequencing batch reactor.
30036752	6	53	theme	community	1173:1181	arg1	diversity					1150:1158	diversity	1150:1158	diversity	1150:1158	Illumina results revealed that the introduction of algae reduced the richness and diversity of bacterial community.
30036752	6	53	theme	community	1173:1181	arg1	richness					1137:1144	richness	1137:1144	richness	1137:1144	Illumina results revealed that the introduction of algae reduced the richness and diversity of bacterial community.
30036752	7	54	from	structure	1208:1216	arg1	communities					1260:1270	bacterial communities	1250:1270	bacterial communities	1250:1270	Besides, few changes in structure and some compositions shifts in bacterial communities were found, while the predominant algae shifted from Diatomea to green algae Chlorophyceae.
30036752	6	55	theme	algae	1119:1123	arg1	introduction					1103:1114	the introduction	1099:1114	the introduction of algae	1099:1123	Illumina results revealed that the introduction of algae reduced the richness and diversity of bacterial community.
30036752	3	56	dep	substances	523:532	arg1	polysaccharides					552:566	polysaccharides	552:566	polysaccharides (PS)	552:571	Occurrence of abundant filamentous bacteria bridging the water-born algae, and overproduction of extracellular polymeric substances (EPS) (especially polysaccharides (PS), tryptophan & protein-like, and humic acid-like substances) were observed on the first 3 days, while the algae grew into the inner side of the granules and mature granules were obtained on day 7.
30036752	3	56	dep	substances	523:532	arg1	substances					621:630	humic acid-like substances	605:630	humic acid-like substances	605:630	Occurrence of abundant filamentous bacteria bridging the water-born algae, and overproduction of extracellular polymeric substances (EPS) (especially polysaccharides (PS), tryptophan & protein-like, and humic acid-like substances) were observed on the first 3 days, while the algae grew into the inner side of the granules and mature granules were obtained on day 7.
30036752	3	56	dep	substances	523:532	arg1	protein-like					587:598	tryptophan & protein-like	574:598	tryptophan & protein-like	574:598	Occurrence of abundant filamentous bacteria bridging the water-born algae, and overproduction of extracellular polymeric substances (EPS) (especially polysaccharides (PS), tryptophan & protein-like, and humic acid-like substances) were observed on the first 3 days, while the algae grew into the inner side of the granules and mature granules were obtained on day 7.
30036752	3	56	dep	substances	523:532	arg1	PS					569:570	PS	569:570	PS	569:570	Occurrence of abundant filamentous bacteria bridging the water-born algae, and overproduction of extracellular polymeric substances (EPS) (especially polysaccharides (PS), tryptophan & protein-like, and humic acid-like substances) were observed on the first 3 days, while the algae grew into the inner side of the granules and mature granules were obtained on day 7.
30036752	5	57	theme	symbiotic	1023:1031	arg1	growth					1033:1038	symbiotic growth	1023:1038	symbiotic growth of algal-bacterial biomass	1023:1065	Whereas, the biomass retention decreased before day 3, and got stable soon with the maturation period with symbiotic growth of algal-bacterial biomass.
30036752	8	58	theme	algal-bacterial	1431:1445	arg1	granules					1447:1454	algal-bacterial granules	1431:1454	algal-bacterial granules	1431:1454	The possible mechanism for natural sunlight induced granulation of algal-bacterial granules was thus proposed based on the interactions between algae and bacteria.
30036752	7	59	from	shifts	1240:1245	arg1	communities					1260:1270	bacterial communities	1250:1270	bacterial communities	1250:1270	Besides, few changes in structure and some compositions shifts in bacterial communities were found, while the predominant algae shifted from Diatomea to green algae Chlorophyceae.
30036752	1	60	theme	Wastewater	145:154	arg1	treatment					156:164	Wastewater treatment	145:164	Wastewater treatment by means of algal-bacterial granules	145:201	Wastewater treatment by means of algal-bacterial granules has become a hot topic worldwide recently.
30036752	3	61	theme	tryptophan	574:583	arg1	protein-like					587:598	tryptophan & protein-like	574:598	tryptophan & protein-like	574:598	Occurrence of abundant filamentous bacteria bridging the water-born algae, and overproduction of extracellular polymeric substances (EPS) (especially polysaccharides (PS), tryptophan & protein-like, and humic acid-like substances) were observed on the first 3 days, while the algae grew into the inner side of the granules and mature granules were obtained on day 7.
30036752	4	62	theme	granules	860:867	arg1	sizes					847:851	the settleability, mean sizes	823:851	the settleability, mean sizes of the granules	823:867	The growth of the water-born algae slightly decreased the settleability, mean sizes of the granules, but stimulated the bioactivity significantly.
30036752	2	63	theme	granules	283:290	arg1	granulation					252:262	Rapid granulation	246:262	Rapid granulation of algal-bacterial granules	246:290	Rapid granulation of algal-bacterial granules was achieved in an aerobic bacterial granular sequencing batch reactor (SBR) under natural sunlight exposure.
30036752	3	64	theme	polymeric	513:521	arg1	EPS					535:537	EPS	535:537	EPS	535:537	Occurrence of abundant filamentous bacteria bridging the water-born algae, and overproduction of extracellular polymeric substances (EPS) (especially polysaccharides (PS), tryptophan & protein-like, and humic acid-like substances) were observed on the first 3 days, while the algae grew into the inner side of the granules and mature granules were obtained on day 7.
30036752	3	64	theme	polymeric	513:521	arg1	substances					523:532	extracellular polymeric substances	499:532	extracellular polymeric substances (EPS) (especially polysaccharides (PS), tryptophan & protein-like, and humic acid-like substances)	499:631	Occurrence of abundant filamentous bacteria bridging the water-born algae, and overproduction of extracellular polymeric substances (EPS) (especially polysaccharides (PS), tryptophan & protein-like, and humic acid-like substances) were observed on the first 3 days, while the algae grew into the inner side of the granules and mature granules were obtained on day 7.
30036752	3	65	located	observed	638:645	arg2	Occurrence					402:411	Occurrence	402:411	Occurrence of abundant filamentous bacteria bridging the water-born algae	402:474	Occurrence of abundant filamentous bacteria bridging the water-born algae, and overproduction of extracellular polymeric substances (EPS) (especially polysaccharides (PS), tryptophan & protein-like, and humic acid-like substances) were observed on the first 3 days, while the algae grew into the inner side of the granules and mature granules were obtained on day 7.
30036752	3	65	located	observed	638:645	arg2	overproduction					481:494	overproduction	481:494	overproduction of extracellular polymeric substances (EPS) (especially polysaccharides (PS), tryptophan & protein-like, and humic acid-like substances)	481:631	Occurrence of abundant filamentous bacteria bridging the water-born algae, and overproduction of extracellular polymeric substances (EPS) (especially polysaccharides (PS), tryptophan & protein-like, and humic acid-like substances) were observed on the first 3 days, while the algae grew into the inner side of the granules and mature granules were obtained on day 7.
30036752	3	65	located	observed	638:645	arg1	days					662:665	the first 3 days	650:665	the first 3 days	650:665	Occurrence of abundant filamentous bacteria bridging the water-born algae, and overproduction of extracellular polymeric substances (EPS) (especially polysaccharides (PS), tryptophan & protein-like, and humic acid-like substances) were observed on the first 3 days, while the algae grew into the inner side of the granules and mature granules were obtained on day 7.
30036752	0	66	theme	granules	71:78	arg1	formation					31:39	rapid formation	25:39	rapid formation of water-born algal-bacterial granules	25:78	Natural sunlight induced rapid formation of water-born algal-bacterial granules in an aerobic bacterial granular photo-sequencing batch reactor.
30036752	2	67	theme	algal-bacterial	267:281	arg1	granules					283:290	algal-bacterial granules	267:290	algal-bacterial granules	267:290	Rapid granulation of algal-bacterial granules was achieved in an aerobic bacterial granular sequencing batch reactor (SBR) under natural sunlight exposure.
30036752	7	68	theme	bacterial	1250:1258	arg1	communities					1260:1270	bacterial communities	1250:1270	bacterial communities	1250:1270	Besides, few changes in structure and some compositions shifts in bacterial communities were found, while the predominant algae shifted from Diatomea to green algae Chlorophyceae.
30036752	7	69	theme	compositions	1227:1238	arg1	shifts					1240:1245	some compositions shifts	1222:1245	some compositions shifts in bacterial communities	1222:1270	Besides, few changes in structure and some compositions shifts in bacterial communities were found, while the predominant algae shifted from Diatomea to green algae Chlorophyceae.
30070847	8	0	theme	ion-trap	1229:1236	arg1	fragmentation					1242:1254	ion-trap MS2 fragmentation	1229:1254	ion-trap MS2 fragmentation	1229:1254	When performing ion-trap MS2 fragmentation, these low-molecular-mass fragments are usually not detected.
30070847	7	1	link	six-linked	1129:1138	arg1	sulfate					1140:1146	six-linked sulfate	1129:1146	six-linked sulfate to GlcNAc	1129:1156	By comparing the fragmentation of low-mass fragments of m/ z 100-320 with standards for six-linked and three-linked sulfate, it could be shown that characteristic fragmentation exists, verifying that porcine gastric mucin contains mostly six-linked sulfate to GlcNAc, whereas human MUC5B contains mostly three-linked Gal.
30070847	7	2	theme	three-linked	1195:1206	arg1	Gal					1208:1210	mostly three-linked Gal	1188:1210	mostly three-linked Gal	1188:1210	By comparing the fragmentation of low-mass fragments of m/ z 100-320 with standards for six-linked and three-linked sulfate, it could be shown that characteristic fragmentation exists, verifying that porcine gastric mucin contains mostly six-linked sulfate to GlcNAc, whereas human MUC5B contains mostly three-linked Gal.
30070847	7	3	theme	characteristic	1039:1052	arg1	fragmentation					1054:1066	characteristic fragmentation	1039:1066	characteristic fragmentation	1039:1066	By comparing the fragmentation of low-mass fragments of m/ z 100-320 with standards for six-linked and three-linked sulfate, it could be shown that characteristic fragmentation exists, verifying that porcine gastric mucin contains mostly six-linked sulfate to GlcNAc, whereas human MUC5B contains mostly three-linked Gal.
30070847	4	4	from	mucins	735:740	arg1	oligosaccharides					672:687	sulfated oligosaccharides	663:687	sulfated oligosaccharides from porcine gastric and human salivary MUC5B mucins	663:740	We adopted higher energy collisional dissociation to characterize sulfated oligosaccharides from porcine gastric and human salivary MUC5B mucins.
30070847	9	5	theme	biological	1371:1380	arg1	questions					1382:1390	biological questions	1371:1390	biological questions of sulfation low-mass fragments are important for the assignment of sulfate position	1371:1475	Hence it can be concluded that to be able to address biological questions of sulfation low-mass fragments are important for the assignment of sulfate position.
30070847	7	6	link	three-linked	994:1005	arg1	sulfate					1007:1013	six-linked and three-linked sulfate	979:1013	six-linked and three-linked sulfate	979:1013	By comparing the fragmentation of low-mass fragments of m/ z 100-320 with standards for six-linked and three-linked sulfate, it could be shown that characteristic fragmentation exists, verifying that porcine gastric mucin contains mostly six-linked sulfate to GlcNAc, whereas human MUC5B contains mostly three-linked Gal.
30070847	7	7	theme	m/	947:948	arg1	z					950:950	m/ z 100-320	947:958	m/ z 100-320	947:958	By comparing the fragmentation of low-mass fragments of m/ z 100-320 with standards for six-linked and three-linked sulfate, it could be shown that characteristic fragmentation exists, verifying that porcine gastric mucin contains mostly six-linked sulfate to GlcNAc, whereas human MUC5B contains mostly three-linked Gal.
30070847	4	8	theme	higher	608:613	arg1	dissociation					634:645	higher energy collisional dissociation	608:645	higher energy collisional dissociation	608:645	We adopted higher energy collisional dissociation to characterize sulfated oligosaccharides from porcine gastric and human salivary MUC5B mucins.
30070847	8	9	theme	MS2	1238:1240	arg1	fragmentation					1242:1254	ion-trap MS2 fragmentation	1229:1254	ion-trap MS2 fragmentation	1229:1254	When performing ion-trap MS2 fragmentation, these low-molecular-mass fragments are usually not detected.
30070847	2	10	theme	oligosaccharides	297:312	arg1	fraction					285:292	only a fraction	278:292	only a fraction of oligosaccharides	278:312	Because only a fraction of oligosaccharides undergo this type of modifications in the Golgi apparatus, sometimes also only by dedicated cells, the glycobiology of these low abundant sulfated oligosaccharides is often overlooked.
30070847	7	11	theme	low-mass	925:932	arg1	fragments					934:942	low-mass fragments	925:942	low-mass fragments of m/ z 100-320	925:958	By comparing the fragmentation of low-mass fragments of m/ z 100-320 with standards for six-linked and three-linked sulfate, it could be shown that characteristic fragmentation exists, verifying that porcine gastric mucin contains mostly six-linked sulfate to GlcNAc, whereas human MUC5B contains mostly three-linked Gal.
30070847	5	12	theme	conclusive	780:789	arg1	spectra					791:797	conclusive spectra	780:797	conclusive spectra up to nonasaccharides	780:819	With this approach we could generate conclusive spectra up to nonasaccharides.
30070847	9	13	theme	sulfation	1395:1403	arg1	questions					1382:1390	biological questions	1371:1390	biological questions of sulfation low-mass fragments are important for the assignment of sulfate position	1371:1475	Hence it can be concluded that to be able to address biological questions of sulfation low-mass fragments are important for the assignment of sulfate position.
30070847	7	14	theme	human	1167:1171	arg1	MUC5B					1173:1177	human MUC5B	1167:1177	human MUC5B	1167:1177	By comparing the fragmentation of low-mass fragments of m/ z 100-320 with standards for six-linked and three-linked sulfate, it could be shown that characteristic fragmentation exists, verifying that porcine gastric mucin contains mostly six-linked sulfate to GlcNAc, whereas human MUC5B contains mostly three-linked Gal.
30070847	6	15	theme	sulfated	845:852	arg1	oligosaccharides					854:869	Both singly and doubly sulfated oligosaccharides	822:869	Both singly and doubly sulfated oligosaccharides	822:869	Both singly and doubly sulfated oligosaccharides were characterized.
30070847	4	16	theme	human	714:718	arg1	mucins					735:740	porcine gastric and human salivary MUC5B mucins	694:740	mucins	735:740	We adopted higher energy collisional dissociation to characterize sulfated oligosaccharides from porcine gastric and human salivary MUC5B mucins.
30070847	9	17	theme	low-mass	1405:1412	arg1	fragments					1414:1422	low-mass fragments	1405:1422	low-mass fragments	1405:1422	Hence it can be concluded that to be able to address biological questions of sulfation low-mass fragments are important for the assignment of sulfate position.
30070847	2	18	theme	sulfated	452:459	arg1	oligosaccharides					461:476	these low abundant sulfated oligosaccharides	433:476	these low abundant sulfated oligosaccharides	433:476	Because only a fraction of oligosaccharides undergo this type of modifications in the Golgi apparatus, sometimes also only by dedicated cells, the glycobiology of these low abundant sulfated oligosaccharides is often overlooked.
30070847	4	19	from	gastric	702:708	arg1	oligosaccharides					672:687	sulfated oligosaccharides	663:687	sulfated oligosaccharides from porcine gastric and human salivary MUC5B mucins	663:740	We adopted higher energy collisional dissociation to characterize sulfated oligosaccharides from porcine gastric and human salivary MUC5B mucins.
30070847	0	20	theme	Energy	7:12	arg1	Spectrometry					44:55	Higher Energy Collisional Dissociation Mass Spectrometry	0:55	Higher Energy Collisional Dissociation Mass Spectrometry of Sulfated O-Linked Oligosaccharides	0:93	Higher Energy Collisional Dissociation Mass Spectrometry of Sulfated O-Linked Oligosaccharides.
30070847	8	21	theme	low-molecular-mass	1263:1280	arg1	fragments					1282:1290	these low-molecular-mass fragments	1257:1290	these low-molecular-mass fragments	1257:1290	When performing ion-trap MS2 fragmentation, these low-molecular-mass fragments are usually not detected.
30070847	2	22	theme	modifications	335:347	arg1	type					327:330	this type	322:330	this type of modifications	322:347	Because only a fraction of oligosaccharides undergo this type of modifications in the Golgi apparatus, sometimes also only by dedicated cells, the glycobiology of these low abundant sulfated oligosaccharides is often overlooked.
30070847	7	23	contain	contains	1113:1120	arg2	sulfate					1140:1146	six-linked sulfate	1129:1146	six-linked sulfate to GlcNAc	1129:1156	By comparing the fragmentation of low-mass fragments of m/ z 100-320 with standards for six-linked and three-linked sulfate, it could be shown that characteristic fragmentation exists, verifying that porcine gastric mucin contains mostly six-linked sulfate to GlcNAc, whereas human MUC5B contains mostly three-linked Gal.
30070847	7	23	contain	contains	1113:1120	arg1	mucin					1107:1111	porcine gastric mucin	1091:1111	porcine gastric mucin	1091:1111	By comparing the fragmentation of low-mass fragments of m/ z 100-320 with standards for six-linked and three-linked sulfate, it could be shown that characteristic fragmentation exists, verifying that porcine gastric mucin contains mostly six-linked sulfate to GlcNAc, whereas human MUC5B contains mostly three-linked Gal.
30070847	2	24	theme	dedicated	396:404	arg1	cells					406:410	dedicated cells	396:410	dedicated cells	396:410	Because only a fraction of oligosaccharides undergo this type of modifications in the Golgi apparatus, sometimes also only by dedicated cells, the glycobiology of these low abundant sulfated oligosaccharides is often overlooked.
30070847	0	25	theme	Higher	0:5	arg1	Spectrometry					44:55	Higher Energy Collisional Dissociation Mass Spectrometry	0:55	Higher Energy Collisional Dissociation Mass Spectrometry of Sulfated O-Linked Oligosaccharides	0:93	Higher Energy Collisional Dissociation Mass Spectrometry of Sulfated O-Linked Oligosaccharides.
30070847	0	26	theme	Dissociation	26:37	arg1	Spectrometry					44:55	Higher Energy Collisional Dissociation Mass Spectrometry	0:55	Higher Energy Collisional Dissociation Mass Spectrometry of Sulfated O-Linked Oligosaccharides	0:93	Higher Energy Collisional Dissociation Mass Spectrometry of Sulfated O-Linked Oligosaccharides.
30070847	9	27	dep	questions	1382:1390	arg1	important					1428:1436	important	1428:1436	important	1428:1436	Hence it can be concluded that to be able to address biological questions of sulfation low-mass fragments are important for the assignment of sulfate position.
30070847	5	28	dep	nonasaccharides	805:819	arg1	up					799:800	up	799:800	up	799:800	With this approach we could generate conclusive spectra up to nonasaccharides.
30070847	7	29	theme	three-linked	994:1005	arg1	sulfate					1007:1013	six-linked and three-linked sulfate	979:1013	six-linked and three-linked sulfate	979:1013	By comparing the fragmentation of low-mass fragments of m/ z 100-320 with standards for six-linked and three-linked sulfate, it could be shown that characteristic fragmentation exists, verifying that porcine gastric mucin contains mostly six-linked sulfate to GlcNAc, whereas human MUC5B contains mostly three-linked Gal.
30070847	1	30	theme	final	113:117	arg1	Sulfation					96:104	Sulfation	96:104	Sulfation	96:104	Sulfation is the final decoration of mucin-type O-linked oligosaccharides before mucins are released into the lumen of the gastrointestinal, respiratory, and genital tracts.
30070847	1	30	theme	final	113:117	arg1	decoration					119:128	the final decoration	109:128	the final decoration of mucin-type O-linked oligosaccharides	109:168	Sulfation is the final decoration of mucin-type O-linked oligosaccharides before mucins are released into the lumen of the gastrointestinal, respiratory, and genital tracts.
30070847	4	31	theme	sulfated	663:670	arg1	oligosaccharides					672:687	sulfated oligosaccharides	663:687	sulfated oligosaccharides from porcine gastric and human salivary MUC5B mucins	663:740	We adopted higher energy collisional dissociation to characterize sulfated oligosaccharides from porcine gastric and human salivary MUC5B mucins.
30070847	2	32	theme	low	439:441	arg1	oligosaccharides					461:476	these low abundant sulfated oligosaccharides	433:476	these low abundant sulfated oligosaccharides	433:476	Because only a fraction of oligosaccharides undergo this type of modifications in the Golgi apparatus, sometimes also only by dedicated cells, the glycobiology of these low abundant sulfated oligosaccharides is often overlooked.
30070847	1	33	theme	gastrointestinal	219:234	arg1	tracts					262:267	the gastrointestinal, respiratory, and genital tracts	215:267	the gastrointestinal, respiratory, and genital tracts	215:267	Sulfation is the final decoration of mucin-type O-linked oligosaccharides before mucins are released into the lumen of the gastrointestinal, respiratory, and genital tracts.
30070847	2	34	theme	Golgi	356:360	arg1	apparatus					362:370	the Golgi apparatus	352:370	the Golgi apparatus	352:370	Because only a fraction of oligosaccharides undergo this type of modifications in the Golgi apparatus, sometimes also only by dedicated cells, the glycobiology of these low abundant sulfated oligosaccharides is often overlooked.
30070847	7	35	theme	six-linked	1129:1138	arg1	sulfate					1140:1146	six-linked sulfate	1129:1146	six-linked sulfate to GlcNAc	1129:1156	By comparing the fragmentation of low-mass fragments of m/ z 100-320 with standards for six-linked and three-linked sulfate, it could be shown that characteristic fragmentation exists, verifying that porcine gastric mucin contains mostly six-linked sulfate to GlcNAc, whereas human MUC5B contains mostly three-linked Gal.
30070847	7	36	link	three-linked	1195:1206	arg1	Gal					1208:1210	mostly three-linked Gal	1188:1210	mostly three-linked Gal	1188:1210	By comparing the fragmentation of low-mass fragments of m/ z 100-320 with standards for six-linked and three-linked sulfate, it could be shown that characteristic fragmentation exists, verifying that porcine gastric mucin contains mostly six-linked sulfate to GlcNAc, whereas human MUC5B contains mostly three-linked Gal.
30070847	0	37	theme	Mass	39:42	arg1	Spectrometry					44:55	Higher Energy Collisional Dissociation Mass Spectrometry	0:55	Higher Energy Collisional Dissociation Mass Spectrometry of Sulfated O-Linked Oligosaccharides	0:93	Higher Energy Collisional Dissociation Mass Spectrometry of Sulfated O-Linked Oligosaccharides.
30070847	2	38	theme	abundant	443:450	arg1	oligosaccharides					461:476	these low abundant sulfated oligosaccharides	433:476	these low abundant sulfated oligosaccharides	433:476	Because only a fraction of oligosaccharides undergo this type of modifications in the Golgi apparatus, sometimes also only by dedicated cells, the glycobiology of these low abundant sulfated oligosaccharides is often overlooked.
30070847	1	39	theme	respiratory	237:247	arg1	tracts					262:267	the gastrointestinal, respiratory, and genital tracts	215:267	the gastrointestinal, respiratory, and genital tracts	215:267	Sulfation is the final decoration of mucin-type O-linked oligosaccharides before mucins are released into the lumen of the gastrointestinal, respiratory, and genital tracts.
30070847	0	40	theme	Sulfated	60:67	arg1	Oligosaccharides					78:93	Sulfated O-Linked Oligosaccharides	60:93	Sulfated O-Linked Oligosaccharides	60:93	Higher Energy Collisional Dissociation Mass Spectrometry of Sulfated O-Linked Oligosaccharides.
30070847	1	41	link	O-linked	144:151	arg1	oligosaccharides					153:168	mucin-type O-linked oligosaccharides	133:168	mucin-type O-linked oligosaccharides	133:168	Sulfation is the final decoration of mucin-type O-linked oligosaccharides before mucins are released into the lumen of the gastrointestinal, respiratory, and genital tracts.
30070847	7	42	theme	porcine	1091:1097	arg1	mucin					1107:1111	porcine gastric mucin	1091:1111	porcine gastric mucin	1091:1111	By comparing the fragmentation of low-mass fragments of m/ z 100-320 with standards for six-linked and three-linked sulfate, it could be shown that characteristic fragmentation exists, verifying that porcine gastric mucin contains mostly six-linked sulfate to GlcNAc, whereas human MUC5B contains mostly three-linked Gal.
30070847	3	43	theme	same	506:509	arg1	time					511:514	the same time	502:514	the same time	502:514	At the same time, the technology to consistently identify and characterize them has been lagging.
30070847	9	44	theme	sulfate	1460:1466	arg1	position					1468:1475	sulfate position	1460:1475	sulfate position	1460:1475	Hence it can be concluded that to be able to address biological questions of sulfation low-mass fragments are important for the assignment of sulfate position.
30070847	7	45	contain	contains	1179:1186	arg2	Gal					1208:1210	mostly three-linked Gal	1188:1210	mostly three-linked Gal	1188:1210	By comparing the fragmentation of low-mass fragments of m/ z 100-320 with standards for six-linked and three-linked sulfate, it could be shown that characteristic fragmentation exists, verifying that porcine gastric mucin contains mostly six-linked sulfate to GlcNAc, whereas human MUC5B contains mostly three-linked Gal.
30070847	7	45	contain	contains	1179:1186	arg1	MUC5B					1173:1177	human MUC5B	1167:1177	human MUC5B	1167:1177	By comparing the fragmentation of low-mass fragments of m/ z 100-320 with standards for six-linked and three-linked sulfate, it could be shown that characteristic fragmentation exists, verifying that porcine gastric mucin contains mostly six-linked sulfate to GlcNAc, whereas human MUC5B contains mostly three-linked Gal.
30070847	1	46	theme	mucin-type	133:142	arg1	oligosaccharides					153:168	mucin-type O-linked oligosaccharides	133:168	mucin-type O-linked oligosaccharides	133:168	Sulfation is the final decoration of mucin-type O-linked oligosaccharides before mucins are released into the lumen of the gastrointestinal, respiratory, and genital tracts.
30070847	9	47	theme	position	1468:1475	arg1	assignment					1446:1455	the assignment	1442:1455	the assignment of sulfate position	1442:1475	Hence it can be concluded that to be able to address biological questions of sulfation low-mass fragments are important for the assignment of sulfate position.
30070847	7	48	theme	gastric	1099:1105	arg1	mucin					1107:1111	porcine gastric mucin	1091:1111	porcine gastric mucin	1091:1111	By comparing the fragmentation of low-mass fragments of m/ z 100-320 with standards for six-linked and three-linked sulfate, it could be shown that characteristic fragmentation exists, verifying that porcine gastric mucin contains mostly six-linked sulfate to GlcNAc, whereas human MUC5B contains mostly three-linked Gal.
30070847	4	49	theme	salivary	720:727	arg1	mucins					735:740	porcine gastric and human salivary MUC5B mucins	694:740	mucins	735:740	We adopted higher energy collisional dissociation to characterize sulfated oligosaccharides from porcine gastric and human salivary MUC5B mucins.
30070847	1	50	theme	O-linked	144:151	arg1	oligosaccharides					153:168	mucin-type O-linked oligosaccharides	133:168	mucin-type O-linked oligosaccharides	133:168	Sulfation is the final decoration of mucin-type O-linked oligosaccharides before mucins are released into the lumen of the gastrointestinal, respiratory, and genital tracts.
30070847	7	51	theme	six-linked	979:988	arg1	sulfate					1007:1013	six-linked and three-linked sulfate	979:1013	six-linked and three-linked sulfate	979:1013	By comparing the fragmentation of low-mass fragments of m/ z 100-320 with standards for six-linked and three-linked sulfate, it could be shown that characteristic fragmentation exists, verifying that porcine gastric mucin contains mostly six-linked sulfate to GlcNAc, whereas human MUC5B contains mostly three-linked Gal.
30070847	4	52	theme	collisional	622:632	arg1	dissociation					634:645	higher energy collisional dissociation	608:645	higher energy collisional dissociation	608:645	We adopted higher energy collisional dissociation to characterize sulfated oligosaccharides from porcine gastric and human salivary MUC5B mucins.
30070847	7	53	theme	fragments	934:942	arg1	fragmentation					908:920	the fragmentation	904:920	the fragmentation of low-mass fragments of m/ z 100-320	904:958	By comparing the fragmentation of low-mass fragments of m/ z 100-320 with standards for six-linked and three-linked sulfate, it could be shown that characteristic fragmentation exists, verifying that porcine gastric mucin contains mostly six-linked sulfate to GlcNAc, whereas human MUC5B contains mostly three-linked Gal.
30070847	1	54	theme	oligosaccharides	153:168	arg1	Sulfation					96:104	Sulfation	96:104	Sulfation	96:104	Sulfation is the final decoration of mucin-type O-linked oligosaccharides before mucins are released into the lumen of the gastrointestinal, respiratory, and genital tracts.
30070847	1	54	theme	oligosaccharides	153:168	arg1	decoration					119:128	the final decoration	109:128	the final decoration of mucin-type O-linked oligosaccharides	109:168	Sulfation is the final decoration of mucin-type O-linked oligosaccharides before mucins are released into the lumen of the gastrointestinal, respiratory, and genital tracts.
30070847	4	55	theme	energy	615:620	arg1	dissociation					634:645	higher energy collisional dissociation	608:645	higher energy collisional dissociation	608:645	We adopted higher energy collisional dissociation to characterize sulfated oligosaccharides from porcine gastric and human salivary MUC5B mucins.
30070847	0	56	theme	Oligosaccharides	78:93	arg1	Spectrometry					44:55	Higher Energy Collisional Dissociation Mass Spectrometry	0:55	Higher Energy Collisional Dissociation Mass Spectrometry of Sulfated O-Linked Oligosaccharides	0:93	Higher Energy Collisional Dissociation Mass Spectrometry of Sulfated O-Linked Oligosaccharides.
30070847	7	57	link	six-linked	979:988	arg1	sulfate					1007:1013	six-linked and three-linked sulfate	979:1013	six-linked and three-linked sulfate	979:1013	By comparing the fragmentation of low-mass fragments of m/ z 100-320 with standards for six-linked and three-linked sulfate, it could be shown that characteristic fragmentation exists, verifying that porcine gastric mucin contains mostly six-linked sulfate to GlcNAc, whereas human MUC5B contains mostly three-linked Gal.
30070847	4	58	theme	MUC5B	729:733	arg1	mucins					735:740	porcine gastric and human salivary MUC5B mucins	694:740	mucins	735:740	We adopted higher energy collisional dissociation to characterize sulfated oligosaccharides from porcine gastric and human salivary MUC5B mucins.
30070847	1	59	theme	genital	254:260	arg1	tracts					262:267	the gastrointestinal, respiratory, and genital tracts	215:267	the gastrointestinal, respiratory, and genital tracts	215:267	Sulfation is the final decoration of mucin-type O-linked oligosaccharides before mucins are released into the lumen of the gastrointestinal, respiratory, and genital tracts.
30070847	0	60	theme	O-Linked	69:76	arg1	Oligosaccharides					78:93	Sulfated O-Linked Oligosaccharides	60:93	Sulfated O-Linked Oligosaccharides	60:93	Higher Energy Collisional Dissociation Mass Spectrometry of Sulfated O-Linked Oligosaccharides.
30070847	0	61	theme	Collisional	14:24	arg1	Spectrometry					44:55	Higher Energy Collisional Dissociation Mass Spectrometry	0:55	Higher Energy Collisional Dissociation Mass Spectrometry of Sulfated O-Linked Oligosaccharides	0:93	Higher Energy Collisional Dissociation Mass Spectrometry of Sulfated O-Linked Oligosaccharides.
30070847	2	62	theme	oligosaccharides	461:476	arg1	glycobiology					417:428	the glycobiology	413:428	the glycobiology of these low abundant sulfated oligosaccharides	413:476	Because only a fraction of oligosaccharides undergo this type of modifications in the Golgi apparatus, sometimes also only by dedicated cells, the glycobiology of these low abundant sulfated oligosaccharides is often overlooked.
30070847	7	63	theme	z	950:950	arg1	fragments					934:942	low-mass fragments	925:942	low-mass fragments of m/ z 100-320	925:958	By comparing the fragmentation of low-mass fragments of m/ z 100-320 with standards for six-linked and three-linked sulfate, it could be shown that characteristic fragmentation exists, verifying that porcine gastric mucin contains mostly six-linked sulfate to GlcNAc, whereas human MUC5B contains mostly three-linked Gal.
30070847	1	64	theme	tracts	262:267	arg1	lumen					206:210	the lumen	202:210	the lumen of the gastrointestinal, respiratory, and genital tracts	202:267	Sulfation is the final decoration of mucin-type O-linked oligosaccharides before mucins are released into the lumen of the gastrointestinal, respiratory, and genital tracts.
30305477	8	0	attach	presence	1317:1324	arg2	residues					1348:1355	putative galactose residues	1329:1355	putative galactose residues	1329:1355	The presence of putative galactose residues in the hypermannosylated neutral N-linked glycans is also discussed.
30305477	8	0	attach	presence	1317:1324	arg1	glycans					1399:1405	the hypermannosylated neutral N-linked glycans	1360:1405	the hypermannosylated neutral N-linked glycans	1360:1405	The presence of putative galactose residues in the hypermannosylated neutral N-linked glycans is also discussed.
30305477	2	1	theme	animals	376:382	arg1	diseases					364:371	several skin diseases	351:371	several skin diseases of animals including humans	351:399	P. antarctica is a commensal organism found in certain plant species, while M. furfur is associated with several skin diseases of animals including humans.
30305477	9	2	link	N-linked	1474:1481	arg1	glycans					1483:1489	the neutral N-linked glycans	1462:1489	the neutral N-linked glycans of P. antarctica and M. furfur	1462:1520	This report is the first to analyze the neutral N-linked glycans of P. antarctica and M. furfur.
30305477	5	3	theme	Manα1-3	796:802	arg1	Manβ1-4GlcNAcβ1-4GlcNAc					834:856	Manα1-2Manα1-6(Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc	781:856	the most abundant neutral N-linked glycan: Manα1-2Manα1-6(Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M8A)	738:862	The two species shared the most abundant neutral N-linked glycan: Manα1-2Manα1-6(Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M8A).
30305477	5	3	theme	Manα1-3	796:802	arg1	M8A					859:861	M8A	859:861	M8A	859:861	The two species shared the most abundant neutral N-linked glycan: Manα1-2Manα1-6(Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M8A).
30305477	7	4	theme	M.	1134:1135	arg1	furfur					1137:1142	M. furfur	1134:1142	M. furfur	1134:1142	In the case of M. furfur, Manα1-2Manα1-6(Manα1-3)Manα1-6(Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M7A) was the second most abundant, while both M8A and M9A were tied for the third most abundant.
30305477	7	5	from	second	1229:1234	arg1	case					1126:1129	the case	1122:1129	the case of M. furfur	1122:1142	In the case of M. furfur, Manα1-2Manα1-6(Manα1-3)Manα1-6(Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M7A) was the second most abundant, while both M8A and M9A were tied for the third most abundant.
30305477	3	6	theme	performance	530:540	arg1	HPLC					565:568	HPLC	565:568	HPLC	565:568	N-linked glycans of P. antarctica and M. furfur were prepared, digested with glycosidases, and structurally analyzed using high performance liquid chromatography (HPLC) and mass spectrometry (MS).
30305477	3	6	theme	performance	530:540	arg1	chromatography					549:562	high performance liquid chromatography	525:562	high performance liquid chromatography (HPLC)	525:569	N-linked glycans of P. antarctica and M. furfur were prepared, digested with glycosidases, and structurally analyzed using high performance liquid chromatography (HPLC) and mass spectrometry (MS).
30305477	5	7	theme	abundant	747:754	arg1	glycan					773:778	the most abundant neutral N-linked glycan	738:778	the most abundant neutral N-linked glycan: Manα1-2Manα1-6(Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M8A)	738:862	The two species shared the most abundant neutral N-linked glycan: Manα1-2Manα1-6(Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M8A).
30305477	6	8	link	N-linked	908:915	arg1	glycans					917:923	The second and third most abundant neutral N-linked glycans	865:923	The second and third most abundant neutral N-linked glycans for P. antarctica	865:941	The second and third most abundant neutral N-linked glycans for P. antarctica were Manα1-2Manα1-6(Manα1-2Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M9A) and Manα1-6(Manα1-3)Manα1-6(Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M5A), respectively.
30305477	6	8	link	N-linked	908:915	arg1	Manβ1-4GlcNAcβ1-4GlcNAc					1008:1030	Manα1-2Manα1-6(Manα1-2Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc	948:1030	Manα1-2Manα1-6(Manα1-2Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M9A)	948:1036	The second and third most abundant neutral N-linked glycans for P. antarctica were Manα1-2Manα1-6(Manα1-2Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M9A) and Manα1-6(Manα1-3)Manα1-6(Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M5A), respectively.
30305477	0	9	link	N-linked	12:19	arg1	glycans					21:27	The neutral N-linked glycans	0:27	The neutral N-linked glycans of the Basidiomycetous yeasts	0:57	The neutral N-linked glycans of the Basidiomycetous yeasts Pseudozyma antarctica and Malassezia furfur (Subphylum Ustilaginomycotina).
30305477	5	10	link	N-linked	764:771	arg1	glycan					773:778	the most abundant neutral N-linked glycan	738:778	the most abundant neutral N-linked glycan: Manα1-2Manα1-6(Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M8A)	738:862	The two species shared the most abundant neutral N-linked glycan: Manα1-2Manα1-6(Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M8A).
30305477	8	11	theme	residues	1348:1355	arg1	presence					1317:1324	The presence	1313:1324	The presence of putative galactose residues in the hypermannosylated neutral N-linked glycans	1313:1405	The presence of putative galactose residues in the hypermannosylated neutral N-linked glycans is also discussed.
30305477	5	12	theme	neutral	756:762	arg1	glycan					773:778	the most abundant neutral N-linked glycan	738:778	the most abundant neutral N-linked glycan: Manα1-2Manα1-6(Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M8A)	738:862	The two species shared the most abundant neutral N-linked glycan: Manα1-2Manα1-6(Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M8A).
30305477	9	13	theme	furfur	1515:1520	arg1	glycans					1483:1489	the neutral N-linked glycans	1462:1489	the neutral N-linked glycans of P. antarctica and M. furfur	1462:1520	This report is the first to analyze the neutral N-linked glycans of P. antarctica and M. furfur.
30305477	3	14	theme	high	525:528	arg1	HPLC					565:568	HPLC	565:568	HPLC	565:568	N-linked glycans of P. antarctica and M. furfur were prepared, digested with glycosidases, and structurally analyzed using high performance liquid chromatography (HPLC) and mass spectrometry (MS).
30305477	3	14	theme	high	525:528	arg1	chromatography					549:562	high performance liquid chromatography	525:562	high performance liquid chromatography (HPLC)	525:569	N-linked glycans of P. antarctica and M. furfur were prepared, digested with glycosidases, and structurally analyzed using high performance liquid chromatography (HPLC) and mass spectrometry (MS).
30305477	6	15	theme	Manα1-6	1042:1048	arg1	M5A					1099:1101	M5A	1099:1101	M5A	1099:1101	The second and third most abundant neutral N-linked glycans for P. antarctica were Manα1-2Manα1-6(Manα1-2Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M9A) and Manα1-6(Manα1-3)Manα1-6(Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M5A), respectively.
30305477	6	15	theme	Manα1-6	1042:1048	arg1	Manβ1-4GlcNAcβ1-4GlcNAc					1074:1096	Manα1-6(Manα1-3)Manα1-6(Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc	1042:1096	Manα1-6(Manα1-3)Manα1-6(Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M5A)	1042:1102	The second and third most abundant neutral N-linked glycans for P. antarctica were Manα1-2Manα1-6(Manα1-2Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M9A) and Manα1-6(Manα1-3)Manα1-6(Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M5A), respectively.
30305477	5	16	theme	N-linked	764:771	arg1	glycan					773:778	the most abundant neutral N-linked glycan	738:778	the most abundant neutral N-linked glycan: Manα1-2Manα1-6(Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M8A)	738:862	The two species shared the most abundant neutral N-linked glycan: Manα1-2Manα1-6(Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M8A).
30305477	6	17	theme	Manα1-3	1066:1072	arg1	M5A					1099:1101	M5A	1099:1101	M5A	1099:1101	The second and third most abundant neutral N-linked glycans for P. antarctica were Manα1-2Manα1-6(Manα1-2Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M9A) and Manα1-6(Manα1-3)Manα1-6(Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M5A), respectively.
30305477	6	17	theme	Manα1-3	1066:1072	arg1	Manβ1-4GlcNAcβ1-4GlcNAc					1074:1096	Manα1-6(Manα1-3)Manα1-6(Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc	1042:1096	Manα1-6(Manα1-3)Manα1-6(Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M5A)	1042:1102	The second and third most abundant neutral N-linked glycans for P. antarctica were Manα1-2Manα1-6(Manα1-2Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M9A) and Manα1-6(Manα1-3)Manα1-6(Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M5A), respectively.
30305477	1	18	theme	basidiomycetous	183:197	arg1	antarctica					146:155	antarctica	146:155	antarctica	146:155	Pseudozyma antarctica and Malassezia furfur are basidiomycetous yeasts under the subphylum Ustilaginomycotina.
30305477	1	18	theme	basidiomycetous	183:197	arg1	furfur					172:177	furfur	172:177	furfur	172:177	Pseudozyma antarctica and Malassezia furfur are basidiomycetous yeasts under the subphylum Ustilaginomycotina.
30305477	1	18	theme	basidiomycetous	183:197	arg1	yeasts					199:204	basidiomycetous yeasts	183:204	basidiomycetous yeasts	183:204	Pseudozyma antarctica and Malassezia furfur are basidiomycetous yeasts under the subphylum Ustilaginomycotina.
30305477	0	19	theme	Basidiomycetous	36:50	arg1	yeasts					52:57	the Basidiomycetous yeasts	32:57	the Basidiomycetous yeasts	32:57	The neutral N-linked glycans of the Basidiomycetous yeasts Pseudozyma antarctica and Malassezia furfur (Subphylum Ustilaginomycotina).
30305477	6	20	theme	N-linked	908:915	arg1	glycans					917:923	The second and third most abundant neutral N-linked glycans	865:923	The second and third most abundant neutral N-linked glycans for P. antarctica	865:941	The second and third most abundant neutral N-linked glycans for P. antarctica were Manα1-2Manα1-6(Manα1-2Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M9A) and Manα1-6(Manα1-3)Manα1-6(Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M5A), respectively.
30305477	6	20	theme	N-linked	908:915	arg1	Manβ1-4GlcNAcβ1-4GlcNAc					1008:1030	Manα1-2Manα1-6(Manα1-2Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc	948:1030	Manα1-2Manα1-6(Manα1-2Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M9A)	948:1036	The second and third most abundant neutral N-linked glycans for P. antarctica were Manα1-2Manα1-6(Manα1-2Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M9A) and Manα1-6(Manα1-3)Manα1-6(Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M5A), respectively.
30305477	2	21	theme	plant	301:305	arg1	species					307:313	certain plant species	293:313	certain plant species	293:313	P. antarctica is a commensal organism found in certain plant species, while M. furfur is associated with several skin diseases of animals including humans.
30305477	7	22	theme	Manα1-2Manα1-3	1176:1189	arg1	Manβ1-4GlcNAcβ1-4GlcNAc					1191:1213	Manα1-2Manα1-6(Manα1-3)Manα1-6(Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc	1145:1213	Manα1-2Manα1-6(Manα1-3)Manα1-6(Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M7A)	1145:1219	In the case of M. furfur, Manα1-2Manα1-6(Manα1-3)Manα1-6(Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M7A) was the second most abundant, while both M8A and M9A were tied for the third most abundant.
30305477	7	22	theme	Manα1-2Manα1-3	1176:1189	arg1	second					1229:1234	second	1229:1234	second	1229:1234	In the case of M. furfur, Manα1-2Manα1-6(Manα1-3)Manα1-6(Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M7A) was the second most abundant, while both M8A and M9A were tied for the third most abundant.
30305477	7	22	theme	Manα1-2Manα1-3	1176:1189	arg1	M7A					1216:1218	M7A	1216:1218	M7A	1216:1218	In the case of M. furfur, Manα1-2Manα1-6(Manα1-3)Manα1-6(Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M7A) was the second most abundant, while both M8A and M9A were tied for the third most abundant.
30305477	0	23	theme	neutral	4:10	arg1	glycans					21:27	The neutral N-linked glycans	0:27	The neutral N-linked glycans of the Basidiomycetous yeasts	0:57	The neutral N-linked glycans of the Basidiomycetous yeasts Pseudozyma antarctica and Malassezia furfur (Subphylum Ustilaginomycotina).
30305477	6	24	theme	neutral	900:906	arg1	glycans					917:923	The second and third most abundant neutral N-linked glycans	865:923	The second and third most abundant neutral N-linked glycans for P. antarctica	865:941	The second and third most abundant neutral N-linked glycans for P. antarctica were Manα1-2Manα1-6(Manα1-2Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M9A) and Manα1-6(Manα1-3)Manα1-6(Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M5A), respectively.
30305477	6	24	theme	neutral	900:906	arg1	Manβ1-4GlcNAcβ1-4GlcNAc					1008:1030	Manα1-2Manα1-6(Manα1-2Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc	948:1030	Manα1-2Manα1-6(Manα1-2Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M9A)	948:1036	The second and third most abundant neutral N-linked glycans for P. antarctica were Manα1-2Manα1-6(Manα1-2Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M9A) and Manα1-6(Manα1-3)Manα1-6(Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M5A), respectively.
30305477	9	25	theme	antarctica	1497:1506	arg1	glycans					1483:1489	the neutral N-linked glycans	1462:1489	the neutral N-linked glycans of P. antarctica and M. furfur	1462:1520	This report is the first to analyze the neutral N-linked glycans of P. antarctica and M. furfur.
30305477	8	26	theme	galactose	1338:1346	arg1	residues					1348:1355	putative galactose residues	1329:1355	putative galactose residues	1329:1355	The presence of putative galactose residues in the hypermannosylated neutral N-linked glycans is also discussed.
30305477	3	27	theme	furfur	443:448	arg1	glycans					411:417	N-linked glycans	402:417	N-linked glycans of P. antarctica and M. furfur	402:448	N-linked glycans of P. antarctica and M. furfur were prepared, digested with glycosidases, and structurally analyzed using high performance liquid chromatography (HPLC) and mass spectrometry (MS).
30305477	8	28	gly	hypermannosylated	1364:1380	arg1	glycans					1399:1405	the hypermannosylated neutral N-linked glycans	1360:1405	the hypermannosylated neutral N-linked glycans	1360:1405	The presence of putative galactose residues in the hypermannosylated neutral N-linked glycans is also discussed.
30305477	8	29	theme	putative	1329:1336	arg1	residues					1348:1355	putative galactose residues	1329:1355	putative galactose residues	1329:1355	The presence of putative galactose residues in the hypermannosylated neutral N-linked glycans is also discussed.
30305477	7	30	theme	Manα1-3	1160:1166	arg1	Manβ1-4GlcNAcβ1-4GlcNAc					1191:1213	Manα1-2Manα1-6(Manα1-3)Manα1-6(Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc	1145:1213	Manα1-2Manα1-6(Manα1-3)Manα1-6(Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M7A)	1145:1219	In the case of M. furfur, Manα1-2Manα1-6(Manα1-3)Manα1-6(Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M7A) was the second most abundant, while both M8A and M9A were tied for the third most abundant.
30305477	7	30	theme	Manα1-3	1160:1166	arg1	second					1229:1234	second	1229:1234	second	1229:1234	In the case of M. furfur, Manα1-2Manα1-6(Manα1-3)Manα1-6(Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M7A) was the second most abundant, while both M8A and M9A were tied for the third most abundant.
30305477	7	30	theme	Manα1-3	1160:1166	arg1	M7A					1216:1218	M7A	1216:1218	M7A	1216:1218	In the case of M. furfur, Manα1-2Manα1-6(Manα1-3)Manα1-6(Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M7A) was the second most abundant, while both M8A and M9A were tied for the third most abundant.
30305477	3	31	theme	mass	575:578	arg1	spectrometry					580:591	mass spectrometry	575:591	mass spectrometry (MS)	575:596	N-linked glycans of P. antarctica and M. furfur were prepared, digested with glycosidases, and structurally analyzed using high performance liquid chromatography (HPLC) and mass spectrometry (MS).
30305477	3	31	theme	mass	575:578	arg1	MS					594:595	MS	594:595	MS	594:595	N-linked glycans of P. antarctica and M. furfur were prepared, digested with glycosidases, and structurally analyzed using high performance liquid chromatography (HPLC) and mass spectrometry (MS).
30305477	8	32	theme	hypermannosylated	1364:1380	arg1	glycans					1399:1405	the hypermannosylated neutral N-linked glycans	1360:1405	the hypermannosylated neutral N-linked glycans	1360:1405	The presence of putative galactose residues in the hypermannosylated neutral N-linked glycans is also discussed.
30305477	1	33	theme	subphylum	216:224	arg1	Ustilaginomycotina					226:243	the subphylum Ustilaginomycotina	212:243	the subphylum Ustilaginomycotina	212:243	Pseudozyma antarctica and Malassezia furfur are basidiomycetous yeasts under the subphylum Ustilaginomycotina.
30305477	0	34	theme	N-linked	12:19	arg1	glycans					21:27	The neutral N-linked glycans	0:27	The neutral N-linked glycans of the Basidiomycetous yeasts	0:57	The neutral N-linked glycans of the Basidiomycetous yeasts Pseudozyma antarctica and Malassezia furfur (Subphylum Ustilaginomycotina).
30305477	6	35	theme	Manα1-2Manα1-2Manα1-3	986:1006	arg1	glycans					917:923	The second and third most abundant neutral N-linked glycans	865:923	The second and third most abundant neutral N-linked glycans for P. antarctica	865:941	The second and third most abundant neutral N-linked glycans for P. antarctica were Manα1-2Manα1-6(Manα1-2Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M9A) and Manα1-6(Manα1-3)Manα1-6(Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M5A), respectively.
30305477	6	35	theme	Manα1-2Manα1-2Manα1-3	986:1006	arg1	M9A					1033:1035	M9A	1033:1035	M9A	1033:1035	The second and third most abundant neutral N-linked glycans for P. antarctica were Manα1-2Manα1-6(Manα1-2Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M9A) and Manα1-6(Manα1-3)Manα1-6(Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M5A), respectively.
30305477	6	35	theme	Manα1-2Manα1-2Manα1-3	986:1006	arg1	Manβ1-4GlcNAcβ1-4GlcNAc					1008:1030	Manα1-2Manα1-6(Manα1-2Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc	948:1030	Manα1-2Manα1-6(Manα1-2Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M9A)	948:1036	The second and third most abundant neutral N-linked glycans for P. antarctica were Manα1-2Manα1-6(Manα1-2Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M9A) and Manα1-6(Manα1-3)Manα1-6(Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M5A), respectively.
30305477	9	36	theme	N-linked	1474:1481	arg1	glycans					1483:1489	the neutral N-linked glycans	1462:1489	the neutral N-linked glycans of P. antarctica and M. furfur	1462:1520	This report is the first to analyze the neutral N-linked glycans of P. antarctica and M. furfur.
30305477	6	37	theme	abundant	891:898	arg1	glycans					917:923	The second and third most abundant neutral N-linked glycans	865:923	The second and third most abundant neutral N-linked glycans for P. antarctica	865:941	The second and third most abundant neutral N-linked glycans for P. antarctica were Manα1-2Manα1-6(Manα1-2Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M9A) and Manα1-6(Manα1-3)Manα1-6(Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M5A), respectively.
30305477	6	37	theme	abundant	891:898	arg1	Manβ1-4GlcNAcβ1-4GlcNAc					1008:1030	Manα1-2Manα1-6(Manα1-2Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc	948:1030	Manα1-2Manα1-6(Manα1-2Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M9A)	948:1036	The second and third most abundant neutral N-linked glycans for P. antarctica were Manα1-2Manα1-6(Manα1-2Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M9A) and Manα1-6(Manα1-3)Manα1-6(Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M5A), respectively.
30305477	2	38	theme	M.	322:323	arg1	furfur					325:330	M. furfur	322:330	M. furfur	322:330	P. antarctica is a commensal organism found in certain plant species, while M. furfur is associated with several skin diseases of animals including humans.
30305477	4	39	theme	N-linked	641:648	arg1	glycans					650:656	neutral N-linked glycans	633:656	neutral N-linked glycans ranging in length from Man3GlcNAc2-PA to Man9GlcNAc2-PA	633:712	Analyses revealed the presence of neutral N-linked glycans ranging in length from Man3GlcNAc2-PA to Man9GlcNAc2-PA.
30305477	7	40	theme	Manα1-2Manα1-6	1145:1158	arg1	Manβ1-4GlcNAcβ1-4GlcNAc					1191:1213	Manα1-2Manα1-6(Manα1-3)Manα1-6(Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc	1145:1213	Manα1-2Manα1-6(Manα1-3)Manα1-6(Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M7A)	1145:1219	In the case of M. furfur, Manα1-2Manα1-6(Manα1-3)Manα1-6(Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M7A) was the second most abundant, while both M8A and M9A were tied for the third most abundant.
30305477	7	40	theme	Manα1-2Manα1-6	1145:1158	arg1	second					1229:1234	second	1229:1234	second	1229:1234	In the case of M. furfur, Manα1-2Manα1-6(Manα1-3)Manα1-6(Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M7A) was the second most abundant, while both M8A and M9A were tied for the third most abundant.
30305477	7	40	theme	Manα1-2Manα1-6	1145:1158	arg1	M7A					1216:1218	M7A	1216:1218	M7A	1216:1218	In the case of M. furfur, Manα1-2Manα1-6(Manα1-3)Manα1-6(Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M7A) was the second most abundant, while both M8A and M9A were tied for the third most abundant.
30305477	9	41	theme	neutral	1466:1472	arg1	glycans					1483:1489	the neutral N-linked glycans	1462:1489	the neutral N-linked glycans of P. antarctica and M. furfur	1462:1520	This report is the first to analyze the neutral N-linked glycans of P. antarctica and M. furfur.
30305477	2	42	theme	commensal	265:273	arg1	antarctica					249:258	antarctica	249:258	antarctica	249:258	P. antarctica is a commensal organism found in certain plant species, while M. furfur is associated with several skin diseases of animals including humans.
30305477	2	42	theme	commensal	265:273	arg1	organism					275:282	a commensal organism	263:282	a commensal organism found in certain plant species	263:313	P. antarctica is a commensal organism found in certain plant species, while M. furfur is associated with several skin diseases of animals including humans.
30305477	7	43	theme	furfur	1137:1142	arg1	case					1126:1129	the case	1122:1129	the case of M. furfur	1122:1142	In the case of M. furfur, Manα1-2Manα1-6(Manα1-3)Manα1-6(Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M7A) was the second most abundant, while both M8A and M9A were tied for the third most abundant.
30305477	5	44	theme	Manα1-6	804:810	arg1	Manβ1-4GlcNAcβ1-4GlcNAc					834:856	Manα1-2Manα1-6(Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc	781:856	the most abundant neutral N-linked glycan: Manα1-2Manα1-6(Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M8A)	738:862	The two species shared the most abundant neutral N-linked glycan: Manα1-2Manα1-6(Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M8A).
30305477	5	44	theme	Manα1-6	804:810	arg1	M8A					859:861	M8A	859:861	M8A	859:861	The two species shared the most abundant neutral N-linked glycan: Manα1-2Manα1-6(Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M8A).
30305477	6	45	theme	Manα1-6	978:984	arg1	glycans					917:923	The second and third most abundant neutral N-linked glycans	865:923	The second and third most abundant neutral N-linked glycans for P. antarctica	865:941	The second and third most abundant neutral N-linked glycans for P. antarctica were Manα1-2Manα1-6(Manα1-2Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M9A) and Manα1-6(Manα1-3)Manα1-6(Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M5A), respectively.
30305477	6	45	theme	Manα1-6	978:984	arg1	M9A					1033:1035	M9A	1033:1035	M9A	1033:1035	The second and third most abundant neutral N-linked glycans for P. antarctica were Manα1-2Manα1-6(Manα1-2Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M9A) and Manα1-6(Manα1-3)Manα1-6(Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M5A), respectively.
30305477	6	45	theme	Manα1-6	978:984	arg1	Manβ1-4GlcNAcβ1-4GlcNAc					1008:1030	Manα1-2Manα1-6(Manα1-2Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc	948:1030	Manα1-2Manα1-6(Manα1-2Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M9A)	948:1036	The second and third most abundant neutral N-linked glycans for P. antarctica were Manα1-2Manα1-6(Manα1-2Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M9A) and Manα1-6(Manα1-3)Manα1-6(Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M5A), respectively.
30305477	6	46	theme	third	880:884	arg1	glycans					917:923	The second and third most abundant neutral N-linked glycans	865:923	The second and third most abundant neutral N-linked glycans for P. antarctica	865:941	The second and third most abundant neutral N-linked glycans for P. antarctica were Manα1-2Manα1-6(Manα1-2Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M9A) and Manα1-6(Manα1-3)Manα1-6(Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M5A), respectively.
30305477	6	46	theme	third	880:884	arg1	Manβ1-4GlcNAcβ1-4GlcNAc					1008:1030	Manα1-2Manα1-6(Manα1-2Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc	948:1030	Manα1-2Manα1-6(Manα1-2Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M9A)	948:1036	The second and third most abundant neutral N-linked glycans for P. antarctica were Manα1-2Manα1-6(Manα1-2Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M9A) and Manα1-6(Manα1-3)Manα1-6(Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M5A), respectively.
30305477	6	47	theme	Manα1-6	1058:1064	arg1	M5A					1099:1101	M5A	1099:1101	M5A	1099:1101	The second and third most abundant neutral N-linked glycans for P. antarctica were Manα1-2Manα1-6(Manα1-2Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M9A) and Manα1-6(Manα1-3)Manα1-6(Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M5A), respectively.
30305477	6	47	theme	Manα1-6	1058:1064	arg1	Manβ1-4GlcNAcβ1-4GlcNAc					1074:1096	Manα1-6(Manα1-3)Manα1-6(Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc	1042:1096	Manα1-6(Manα1-3)Manα1-6(Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M5A)	1042:1102	The second and third most abundant neutral N-linked glycans for P. antarctica were Manα1-2Manα1-6(Manα1-2Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M9A) and Manα1-6(Manα1-3)Manα1-6(Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M5A), respectively.
30305477	0	48	theme	yeasts	52:57	arg1	glycans					21:27	The neutral N-linked glycans	0:27	The neutral N-linked glycans of the Basidiomycetous yeasts	0:57	The neutral N-linked glycans of the Basidiomycetous yeasts Pseudozyma antarctica and Malassezia furfur (Subphylum Ustilaginomycotina).
30305477	7	49	from	case	1126:1129	arg1	Manβ1-4GlcNAcβ1-4GlcNAc					1191:1213	Manα1-2Manα1-6(Manα1-3)Manα1-6(Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc	1145:1213	Manα1-2Manα1-6(Manα1-3)Manα1-6(Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M7A)	1145:1219	In the case of M. furfur, Manα1-2Manα1-6(Manα1-3)Manα1-6(Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M7A) was the second most abundant, while both M8A and M9A were tied for the third most abundant.
30305477	7	49	from	case	1126:1129	arg1	second					1229:1234	second	1229:1234	second	1229:1234	In the case of M. furfur, Manα1-2Manα1-6(Manα1-3)Manα1-6(Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M7A) was the second most abundant, while both M8A and M9A were tied for the third most abundant.
30305477	5	50	theme	Manα1-2Manα1-2Manα1-3	812:832	arg1	Manβ1-4GlcNAcβ1-4GlcNAc					834:856	Manα1-2Manα1-6(Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc	781:856	the most abundant neutral N-linked glycan: Manα1-2Manα1-6(Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M8A)	738:862	The two species shared the most abundant neutral N-linked glycan: Manα1-2Manα1-6(Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M8A).
30305477	5	50	theme	Manα1-2Manα1-2Manα1-3	812:832	arg1	M8A					859:861	M8A	859:861	M8A	859:861	The two species shared the most abundant neutral N-linked glycan: Manα1-2Manα1-6(Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M8A).
30305477	8	51	theme	N-linked	1390:1397	arg1	glycans					1399:1405	the hypermannosylated neutral N-linked glycans	1360:1405	the hypermannosylated neutral N-linked glycans	1360:1405	The presence of putative galactose residues in the hypermannosylated neutral N-linked glycans is also discussed.
30305477	3	52	theme	antarctica	425:434	arg1	glycans					411:417	N-linked glycans	402:417	N-linked glycans of P. antarctica and M. furfur	402:448	N-linked glycans of P. antarctica and M. furfur were prepared, digested with glycosidases, and structurally analyzed using high performance liquid chromatography (HPLC) and mass spectrometry (MS).
30305477	6	53	theme	Manα1-2Manα1-3	963:976	arg1	glycans					917:923	The second and third most abundant neutral N-linked glycans	865:923	The second and third most abundant neutral N-linked glycans for P. antarctica	865:941	The second and third most abundant neutral N-linked glycans for P. antarctica were Manα1-2Manα1-6(Manα1-2Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M9A) and Manα1-6(Manα1-3)Manα1-6(Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M5A), respectively.
30305477	6	53	theme	Manα1-2Manα1-3	963:976	arg1	M9A					1033:1035	M9A	1033:1035	M9A	1033:1035	The second and third most abundant neutral N-linked glycans for P. antarctica were Manα1-2Manα1-6(Manα1-2Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M9A) and Manα1-6(Manα1-3)Manα1-6(Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M5A), respectively.
30305477	6	53	theme	Manα1-2Manα1-3	963:976	arg1	Manβ1-4GlcNAcβ1-4GlcNAc					1008:1030	Manα1-2Manα1-6(Manα1-2Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc	948:1030	Manα1-2Manα1-6(Manα1-2Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M9A)	948:1036	The second and third most abundant neutral N-linked glycans for P. antarctica were Manα1-2Manα1-6(Manα1-2Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M9A) and Manα1-6(Manα1-3)Manα1-6(Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M5A), respectively.
30305477	5	54	dep	glycan	773:778	arg1	Manβ1-4GlcNAcβ1-4GlcNAc					834:856	Manα1-2Manα1-6(Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc	781:856	the most abundant neutral N-linked glycan: Manα1-2Manα1-6(Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M8A)	738:862	The two species shared the most abundant neutral N-linked glycan: Manα1-2Manα1-6(Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M8A).
30305477	5	54	dep	glycan	773:778	arg1	M8A					859:861	M8A	859:861	M8A	859:861	The two species shared the most abundant neutral N-linked glycan: Manα1-2Manα1-6(Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M8A).
30305477	6	55	theme	second	869:874	arg1	glycans					917:923	The second and third most abundant neutral N-linked glycans	865:923	The second and third most abundant neutral N-linked glycans for P. antarctica	865:941	The second and third most abundant neutral N-linked glycans for P. antarctica were Manα1-2Manα1-6(Manα1-2Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M9A) and Manα1-6(Manα1-3)Manα1-6(Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M5A), respectively.
30305477	6	55	theme	second	869:874	arg1	Manβ1-4GlcNAcβ1-4GlcNAc					1008:1030	Manα1-2Manα1-6(Manα1-2Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc	948:1030	Manα1-2Manα1-6(Manα1-2Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M9A)	948:1036	The second and third most abundant neutral N-linked glycans for P. antarctica were Manα1-2Manα1-6(Manα1-2Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M9A) and Manα1-6(Manα1-3)Manα1-6(Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M5A), respectively.
30305477	8	56	theme	neutral	1382:1388	arg1	glycans					1399:1405	the hypermannosylated neutral N-linked glycans	1360:1405	the hypermannosylated neutral N-linked glycans	1360:1405	The presence of putative galactose residues in the hypermannosylated neutral N-linked glycans is also discussed.
30305477	2	57	theme	skin	359:362	arg1	diseases					364:371	several skin diseases	351:371	several skin diseases of animals including humans	351:399	P. antarctica is a commensal organism found in certain plant species, while M. furfur is associated with several skin diseases of animals including humans.
30305477	4	58	link	N-linked	641:648	arg1	glycans					650:656	neutral N-linked glycans	633:656	neutral N-linked glycans ranging in length from Man3GlcNAc2-PA to Man9GlcNAc2-PA	633:712	Analyses revealed the presence of neutral N-linked glycans ranging in length from Man3GlcNAc2-PA to Man9GlcNAc2-PA.
30305477	2	59	theme	certain	293:299	arg1	species					307:313	certain plant species	293:313	certain plant species	293:313	P. antarctica is a commensal organism found in certain plant species, while M. furfur is associated with several skin diseases of animals including humans.
30305477	2	60	theme	several	351:357	arg1	diseases					364:371	several skin diseases	351:371	several skin diseases of animals including humans	351:399	P. antarctica is a commensal organism found in certain plant species, while M. furfur is associated with several skin diseases of animals including humans.
30305477	0	61	dep	antarctica	70:79	arg1	glycans					21:27	The neutral N-linked glycans	0:27	The neutral N-linked glycans of the Basidiomycetous yeasts	0:57	The neutral N-linked glycans of the Basidiomycetous yeasts Pseudozyma antarctica and Malassezia furfur (Subphylum Ustilaginomycotina).
30305477	0	61	dep	antarctica	70:79	arg1	Ustilaginomycotina					114:131	Subphylum Ustilaginomycotina	104:131	Subphylum Ustilaginomycotina	104:131	The neutral N-linked glycans of the Basidiomycetous yeasts Pseudozyma antarctica and Malassezia furfur (Subphylum Ustilaginomycotina).
30305477	8	62	link	N-linked	1390:1397	arg1	glycans					1399:1405	the hypermannosylated neutral N-linked glycans	1360:1405	the hypermannosylated neutral N-linked glycans	1360:1405	The presence of putative galactose residues in the hypermannosylated neutral N-linked glycans is also discussed.
30305477	3	63	link	N-linked	402:409	arg1	glycans					411:417	N-linked glycans	402:417	N-linked glycans of P. antarctica and M. furfur	402:448	N-linked glycans of P. antarctica and M. furfur were prepared, digested with glycosidases, and structurally analyzed using high performance liquid chromatography (HPLC) and mass spectrometry (MS).
30305477	6	64	theme	Manα1-3	1050:1056	arg1	M5A					1099:1101	M5A	1099:1101	M5A	1099:1101	The second and third most abundant neutral N-linked glycans for P. antarctica were Manα1-2Manα1-6(Manα1-2Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M9A) and Manα1-6(Manα1-3)Manα1-6(Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M5A), respectively.
30305477	6	64	theme	Manα1-3	1050:1056	arg1	Manβ1-4GlcNAcβ1-4GlcNAc					1074:1096	Manα1-6(Manα1-3)Manα1-6(Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc	1042:1096	Manα1-6(Manα1-3)Manα1-6(Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M5A)	1042:1102	The second and third most abundant neutral N-linked glycans for P. antarctica were Manα1-2Manα1-6(Manα1-2Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M9A) and Manα1-6(Manα1-3)Manα1-6(Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M5A), respectively.
30305477	4	65	theme	glycans	650:656	arg1	presence					621:628	the presence	617:628	the presence of neutral N-linked glycans ranging in length from Man3GlcNAc2-PA to Man9GlcNAc2-PA	617:712	Analyses revealed the presence of neutral N-linked glycans ranging in length from Man3GlcNAc2-PA to Man9GlcNAc2-PA.
30305477	3	66	theme	liquid	542:547	arg1	HPLC					565:568	HPLC	565:568	HPLC	565:568	N-linked glycans of P. antarctica and M. furfur were prepared, digested with glycosidases, and structurally analyzed using high performance liquid chromatography (HPLC) and mass spectrometry (MS).
30305477	3	66	theme	liquid	542:547	arg1	chromatography					549:562	high performance liquid chromatography	525:562	high performance liquid chromatography (HPLC)	525:569	N-linked glycans of P. antarctica and M. furfur were prepared, digested with glycosidases, and structurally analyzed using high performance liquid chromatography (HPLC) and mass spectrometry (MS).
30305477	8	67	from	presence	1317:1324	arg1	glycans					1399:1405	the hypermannosylated neutral N-linked glycans	1360:1405	the hypermannosylated neutral N-linked glycans	1360:1405	The presence of putative galactose residues in the hypermannosylated neutral N-linked glycans is also discussed.
30305477	4	68	theme	neutral	633:639	arg1	glycans					650:656	neutral N-linked glycans	633:656	neutral N-linked glycans ranging in length from Man3GlcNAc2-PA to Man9GlcNAc2-PA	633:712	Analyses revealed the presence of neutral N-linked glycans ranging in length from Man3GlcNAc2-PA to Man9GlcNAc2-PA.
30305477	5	69	theme	Manα1-2Manα1-6	781:794	arg1	Manβ1-4GlcNAcβ1-4GlcNAc					834:856	Manα1-2Manα1-6(Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc	781:856	the most abundant neutral N-linked glycan: Manα1-2Manα1-6(Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M8A)	738:862	The two species shared the most abundant neutral N-linked glycan: Manα1-2Manα1-6(Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M8A).
30305477	5	69	theme	Manα1-2Manα1-6	781:794	arg1	M8A					859:861	M8A	859:861	M8A	859:861	The two species shared the most abundant neutral N-linked glycan: Manα1-2Manα1-6(Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M8A).
30305477	6	70	theme	Manα1-2Manα1-6	948:961	arg1	glycans					917:923	The second and third most abundant neutral N-linked glycans	865:923	The second and third most abundant neutral N-linked glycans for P. antarctica	865:941	The second and third most abundant neutral N-linked glycans for P. antarctica were Manα1-2Manα1-6(Manα1-2Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M9A) and Manα1-6(Manα1-3)Manα1-6(Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M5A), respectively.
30305477	6	70	theme	Manα1-2Manα1-6	948:961	arg1	M9A					1033:1035	M9A	1033:1035	M9A	1033:1035	The second and third most abundant neutral N-linked glycans for P. antarctica were Manα1-2Manα1-6(Manα1-2Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M9A) and Manα1-6(Manα1-3)Manα1-6(Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M5A), respectively.
30305477	6	70	theme	Manα1-2Manα1-6	948:961	arg1	Manβ1-4GlcNAcβ1-4GlcNAc					1008:1030	Manα1-2Manα1-6(Manα1-2Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc	948:1030	Manα1-2Manα1-6(Manα1-2Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M9A)	948:1036	The second and third most abundant neutral N-linked glycans for P. antarctica were Manα1-2Manα1-6(Manα1-2Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M9A) and Manα1-6(Manα1-3)Manα1-6(Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M5A), respectively.
30305477	3	71	theme	N-linked	402:409	arg1	glycans					411:417	N-linked glycans	402:417	N-linked glycans of P. antarctica and M. furfur	402:448	N-linked glycans of P. antarctica and M. furfur were prepared, digested with glycosidases, and structurally analyzed using high performance liquid chromatography (HPLC) and mass spectrometry (MS).
30305477	7	72	theme	Manα1-6	1168:1174	arg1	Manβ1-4GlcNAcβ1-4GlcNAc					1191:1213	Manα1-2Manα1-6(Manα1-3)Manα1-6(Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc	1145:1213	Manα1-2Manα1-6(Manα1-3)Manα1-6(Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M7A)	1145:1219	In the case of M. furfur, Manα1-2Manα1-6(Manα1-3)Manα1-6(Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M7A) was the second most abundant, while both M8A and M9A were tied for the third most abundant.
30305477	7	72	theme	Manα1-6	1168:1174	arg1	second					1229:1234	second	1229:1234	second	1229:1234	In the case of M. furfur, Manα1-2Manα1-6(Manα1-3)Manα1-6(Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M7A) was the second most abundant, while both M8A and M9A were tied for the third most abundant.
30305477	7	72	theme	Manα1-6	1168:1174	arg1	M7A					1216:1218	M7A	1216:1218	M7A	1216:1218	In the case of M. furfur, Manα1-2Manα1-6(Manα1-3)Manα1-6(Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M7A) was the second most abundant, while both M8A and M9A were tied for the third most abundant.
30305477	2	73	located	found	284:288	arg2	antarctica					249:258	antarctica	249:258	antarctica	249:258	P. antarctica is a commensal organism found in certain plant species, while M. furfur is associated with several skin diseases of animals including humans.
30305477	2	73	located	found	284:288	arg1	species					307:313	certain plant species	293:313	certain plant species	293:313	P. antarctica is a commensal organism found in certain plant species, while M. furfur is associated with several skin diseases of animals including humans.
30305477	2	73	located	found	284:288	arg2	organism					275:282	a commensal organism	263:282	a commensal organism found in certain plant species	263:313	P. antarctica is a commensal organism found in certain plant species, while M. furfur is associated with several skin diseases of animals including humans.
30355697	7	0	used	used	1004:1007	arg2	antigens					982:989	The reference antigens	968:989	The reference antigens studied	968:997	The reference antigens studied were used in the 2014-2015 influenza season and included A/California/07/2009 H1N1, A/Texas/50/2012 H3N2, and B/Massachusetts/02/2012.
30355697	11	1	theme	occupying	1552:1560	arg1	glycan					1562:1567	their occupying glycan	1546:1567	their occupying glycan to functional regions, including antigenic sites, lectin interaction regions, and fusion domains,	1546:1665	Mapping of glycosites with their occupying glycan to functional regions, including antigenic sites, lectin interaction regions, and fusion domains, was performed and has implications for immune processing, immune responses, and antigenic shielding.
30355697	13	2	from	Differences	2204:2214	arg1	heterogeneity					2268:2280	heterogeneity	2268:2280	heterogeneity	2268:2280	Differences in hemagglutinin glycosylation site composition and heterogeneity seen in antigens produced in different cell substrates suggest differences in processing and downstream immune responses.
30355697	13	2	from	Differences	2204:2214	arg1	composition					2252:2262	hemagglutinin glycosylation site composition	2219:2262	hemagglutinin glycosylation site composition	2219:2262	Differences in hemagglutinin glycosylation site composition and heterogeneity seen in antigens produced in different cell substrates suggest differences in processing and downstream immune responses.
30355697	9	3	theme	antigen	1328:1334	arg1	glycosylation					1336:1348	HA antigen glycosylation	1325:1348	HA antigen glycosylation	1325:1348	We also examined the sensitivity of the single radial immunodiffusion (SRID) potency test to differences in HA antigen glycosylation.
30355697	12	4	gly	glycosylation	1923:1935	arg1	B/Massachusetts/02/2012					2059:2081	B/Massachusetts/02/2012	2059:2081	B/Massachusetts/02/2012	2059:2081	Differences in glycosylation patterns, as dictated by the cell system used for expression, may impact these functions.IMPORTANCE In the present study, the glycosylation patterns of the 2014-2015 influenza vaccine season standard antigens A/California/07/2009 H1N1, A/Texas/50/2012 H3N2, and B/Massachusetts/02/2012 were revealed, and the sensitivity of the single radial immunodiffusion (SRID) potency test to glycosylation was tested.
30355697	12	4	gly	glycosylation	1923:1935	arg1	H3N2					2049:2052	H3N2	2049:2052	H3N2	2049:2052	Differences in glycosylation patterns, as dictated by the cell system used for expression, may impact these functions.IMPORTANCE In the present study, the glycosylation patterns of the 2014-2015 influenza vaccine season standard antigens A/California/07/2009 H1N1, A/Texas/50/2012 H3N2, and B/Massachusetts/02/2012 were revealed, and the sensitivity of the single radial immunodiffusion (SRID) potency test to glycosylation was tested.
30355697	12	4	gly	glycosylation	1923:1935	arg1	antigens					1997:2004	the 2014-2015 influenza vaccine season standard antigens	1949:2004	the 2014-2015 influenza vaccine season standard antigens A/California/07/2009 H1N1, A/Texas/50/2012 H3N2, and B/Massachusetts/02/2012	1949:2081	Differences in glycosylation patterns, as dictated by the cell system used for expression, may impact these functions.IMPORTANCE In the present study, the glycosylation patterns of the 2014-2015 influenza vaccine season standard antigens A/California/07/2009 H1N1, A/Texas/50/2012 H3N2, and B/Massachusetts/02/2012 were revealed, and the sensitivity of the single radial immunodiffusion (SRID) potency test to glycosylation was tested.
30355697	1	5	theme	type	256:259	arg1	strains					199:205	three or four virus strains	179:205	three or four virus strains for inclusion in the annual influenza vaccine: a type A:H1N1 virus, a type A:H3N2 virus, and one or two type B viruses	179:324	Prior to each annual flu season, health authorities recommend three or four virus strains for inclusion in the annual influenza vaccine: a type A:H1N1 virus, a type A:H3N2 virus, and one or two type B viruses.
30355697	1	5	theme	type	256:259	arg1	virus					268:272	a type A:H1N1 virus	254:272	a type A:H1N1 virus	254:272	Prior to each annual flu season, health authorities recommend three or four virus strains for inclusion in the annual influenza vaccine: a type A:H1N1 virus, a type A:H3N2 virus, and one or two type B viruses.
30355697	12	6	theme	glycosylation	1923:1935	arg1	patterns					1937:1944	the glycosylation patterns	1919:1944	the glycosylation patterns of the 2014-2015 influenza vaccine season standard antigens A/California/07/2009 H1N1, A/Texas/50/2012 H3N2, and B/Massachusetts/02/2012	1919:2081	Differences in glycosylation patterns, as dictated by the cell system used for expression, may impact these functions.IMPORTANCE In the present study, the glycosylation patterns of the 2014-2015 influenza vaccine season standard antigens A/California/07/2009 H1N1, A/Texas/50/2012 H3N2, and B/Massachusetts/02/2012 were revealed, and the sensitivity of the single radial immunodiffusion (SRID) potency test to glycosylation was tested.
30355697	13	7	theme	hemagglutinin	2219:2231	arg1	composition					2252:2262	hemagglutinin glycosylation site composition	2219:2262	hemagglutinin glycosylation site composition	2219:2262	Differences in hemagglutinin glycosylation site composition and heterogeneity seen in antigens produced in different cell substrates suggest differences in processing and downstream immune responses.
30355697	8	8	theme	glycan	1143:1148	arg1	patterns					1193:1200	Released glycan and HA-specific glycopeptide glycosylation patterns	1134:1200	Released glycan and HA-specific glycopeptide glycosylation patterns	1134:1200	Released glycan and HA-specific glycopeptide glycosylation patterns were examined.
30355697	10	9	theme	glycosylation	1476:1488	arg1	status					1490:1495	any HA antigen glycosylation status	1461:1495	any HA antigen glycosylation status from any cell system	1461:1516	Based on deglycosylation studies applied using standard assay procedures, the SRID assay was not sensitive to any HA antigen glycosylation status from any cell system.
30355697	11	10	with	glycosites	1530:1539	arg1	glycan					1562:1567	their occupying glycan	1546:1567	their occupying glycan to functional regions, including antigenic sites, lectin interaction regions, and fusion domains,	1546:1665	Mapping of glycosites with their occupying glycan to functional regions, including antigenic sites, lectin interaction regions, and fusion domains, was performed and has implications for immune processing, immune responses, and antigenic shielding.
30355697	7	11	theme	reference	972:980	arg1	antigens					982:989	The reference antigens	968:989	The reference antigens studied	968:997	The reference antigens studied were used in the 2014-2015 influenza season and included A/California/07/2009 H1N1, A/Texas/50/2012 H3N2, and B/Massachusetts/02/2012.
30355697	4	12	theme	vaccine	760:766	arg1	testing					768:774	vaccine testing	760:774	vaccine testing	760:774	These reagents are supplied by the Center for Biologics Evaluation and Research (CBER) or the National Institute for Biological Standards and Control (NIBSC) for use in vaccine testing.
30355697	8	13	theme	HA-specific	1154:1164	arg1	patterns					1193:1200	Released glycan and HA-specific glycopeptide glycosylation patterns	1134:1200	Released glycan and HA-specific glycopeptide glycosylation patterns	1134:1200	Released glycan and HA-specific glycopeptide glycosylation patterns were examined.
30355697	15	14	theme	vaccine	2657:2663	arg1	preparation					2673:2683	vaccine antigen preparation	2657:2683	vaccine antigen preparation	2657:2683	This work reveals important differences in vaccine antigens and may point out areas where improvements may be made concerning vaccine antigen preparation, immune processing, and testing.
30355697	13	15	theme	different	2311:2319	arg1	substrates					2326:2335	different cell substrates	2311:2335	different cell substrates	2311:2335	Differences in hemagglutinin glycosylation site composition and heterogeneity seen in antigens produced in different cell substrates suggest differences in processing and downstream immune responses.
30355697	12	16	theme	present	1904:1910	arg1	study					1912:1916	the present study	1900:1916	the present study	1900:1916	Differences in glycosylation patterns, as dictated by the cell system used for expression, may impact these functions.IMPORTANCE In the present study, the glycosylation patterns of the 2014-2015 influenza vaccine season standard antigens A/California/07/2009 H1N1, A/Texas/50/2012 H3N2, and B/Massachusetts/02/2012 were revealed, and the sensitivity of the single radial immunodiffusion (SRID) potency test to glycosylation was tested.
30355697	2	17	located	found	369:373	arg1	patterns					396:403	the glycosylation patterns	378:403	the glycosylation patterns of the major influenza virus antigen, hemagglutinin (HA)	378:460	Antigenic differences between strains are found in the glycosylation patterns of the major influenza virus antigen, hemagglutinin (HA).
30355697	2	17	located	found	369:373	arg2	differences					337:347	Antigenic differences	327:347	Antigenic differences between strains	327:363	Antigenic differences between strains are found in the glycosylation patterns of the major influenza virus antigen, hemagglutinin (HA).
30355697	8	18	theme	glycosylation	1179:1191	arg1	patterns					1193:1200	Released glycan and HA-specific glycopeptide glycosylation patterns	1134:1200	Released glycan and HA-specific glycopeptide glycosylation patterns	1134:1200	Released glycan and HA-specific glycopeptide glycosylation patterns were examined.
30355697	10	19	theme	cell	1506:1509	arg1	system					1511:1516	any cell system	1502:1516	any cell system	1502:1516	Based on deglycosylation studies applied using standard assay procedures, the SRID assay was not sensitive to any HA antigen glycosylation status from any cell system.
30355697	11	20	theme	immune	1706:1711	arg1	processing					1713:1722	immune processing	1706:1722	immune processing	1706:1722	Mapping of glycosites with their occupying glycan to functional regions, including antigenic sites, lectin interaction regions, and fusion domains, was performed and has implications for immune processing, immune responses, and antigenic shielding.
30355697	1	21	theme	type	311:314	arg1	viruses					318:324	one or two type B viruses	300:324	one or two type B viruses	300:324	Prior to each annual flu season, health authorities recommend three or four virus strains for inclusion in the annual influenza vaccine: a type A:H1N1 virus, a type A:H3N2 virus, and one or two type B viruses.
30355697	12	22	theme	antigens	1997:2004	arg1	patterns					1937:1944	the glycosylation patterns	1919:1944	the glycosylation patterns of the 2014-2015 influenza vaccine season standard antigens A/California/07/2009 H1N1, A/Texas/50/2012 H3N2, and B/Massachusetts/02/2012	1919:2081	Differences in glycosylation patterns, as dictated by the cell system used for expression, may impact these functions.IMPORTANCE In the present study, the glycosylation patterns of the 2014-2015 influenza vaccine season standard antigens A/California/07/2009 H1N1, A/Texas/50/2012 H3N2, and B/Massachusetts/02/2012 were revealed, and the sensitivity of the single radial immunodiffusion (SRID) potency test to glycosylation was tested.
30355697	3	23	theme	antigens	525:532	arg1	patterns					497:504	the glycosylation patterns	479:504	the glycosylation patterns of seven reference antigens containing HA used in influenza vaccine potency testing	479:588	Here we examine the glycosylation patterns of seven reference antigens containing HA used in influenza vaccine potency testing.
30355697	12	24	from	Differences	1768:1778	arg1	patterns					1797:1804	glycosylation patterns	1783:1804	glycosylation patterns	1783:1804	Differences in glycosylation patterns, as dictated by the cell system used for expression, may impact these functions.IMPORTANCE In the present study, the glycosylation patterns of the 2014-2015 influenza vaccine season standard antigens A/California/07/2009 H1N1, A/Texas/50/2012 H3N2, and B/Massachusetts/02/2012 were revealed, and the sensitivity of the single radial immunodiffusion (SRID) potency test to glycosylation was tested.
30355697	4	25	theme	Biologics	637:645	arg1	Evaluation					647:656	Biologics Evaluation	637:656	Biologics Evaluation	637:656	These reagents are supplied by the Center for Biologics Evaluation and Research (CBER) or the National Institute for Biological Standards and Control (NIBSC) for use in vaccine testing.
30355697	8	26	gly	glycopeptide	1166:1177	arg2	glycopeptide					1166:1177	Released glycan and HA-specific glycopeptide glycosylation patterns	1134:1200	Released glycan and HA-specific glycopeptide glycosylation patterns	1134:1200	Released glycan and HA-specific glycopeptide glycosylation patterns were examined.
30355697	5	27	theme	canine	816:821	arg1	MDCK					831:834	MDCK	831:834	MDCK	831:834	Those produced in hen egg, Madin-Darby canine kidney (MDCK), and insect (Sf9) expression systems were examined.
30355697	5	27	theme	canine	816:821	arg1	kidney					823:828	Madin-Darby canine kidney	804:828	Madin-Darby canine kidney (MDCK)	804:835	Those produced in hen egg, Madin-Darby canine kidney (MDCK), and insect (Sf9) expression systems were examined.
30355697	1	28	theme	flu	138:140	arg1	season					142:147	each annual flu season	126:147	each annual flu season	126:147	Prior to each annual flu season, health authorities recommend three or four virus strains for inclusion in the annual influenza vaccine: a type A:H1N1 virus, a type A:H3N2 virus, and one or two type B viruses.
30355697	13	29	theme	site	2247:2250	arg1	composition					2252:2262	hemagglutinin glycosylation site composition	2219:2262	hemagglutinin glycosylation site composition	2219:2262	Differences in hemagglutinin glycosylation site composition and heterogeneity seen in antigens produced in different cell substrates suggest differences in processing and downstream immune responses.
30355697	2	30	theme	antigen	434:440	arg1	patterns					396:403	the glycosylation patterns	378:403	the glycosylation patterns of the major influenza virus antigen, hemagglutinin (HA)	378:460	Antigenic differences between strains are found in the glycosylation patterns of the major influenza virus antigen, hemagglutinin (HA).
30355697	12	31	theme	vaccine	1973:1979	arg1	season					1981:1986	the 2014-2015 influenza vaccine season	1949:1986	the 2014-2015 influenza vaccine season standard antigens A/California/07/2009 H1N1, A/Texas/50/2012 H3N2, and B/Massachusetts/02/2012	1949:2081	Differences in glycosylation patterns, as dictated by the cell system used for expression, may impact these functions.IMPORTANCE In the present study, the glycosylation patterns of the 2014-2015 influenza vaccine season standard antigens A/California/07/2009 H1N1, A/Texas/50/2012 H3N2, and B/Massachusetts/02/2012 were revealed, and the sensitivity of the single radial immunodiffusion (SRID) potency test to glycosylation was tested.
30355697	2	32	theme	influenza	418:426	arg1	hemagglutinin					443:455	hemagglutinin	443:455	hemagglutinin (HA)	443:460	Antigenic differences between strains are found in the glycosylation patterns of the major influenza virus antigen, hemagglutinin (HA).
30355697	2	32	theme	influenza	418:426	arg1	antigen					434:440	the major influenza virus antigen	408:440	the major influenza virus antigen	408:440	Antigenic differences between strains are found in the glycosylation patterns of the major influenza virus antigen, hemagglutinin (HA).
30355697	9	33	theme	radial	1264:1269	arg1	SRID					1288:1291	SRID	1288:1291	SRID	1288:1291	We also examined the sensitivity of the single radial immunodiffusion (SRID) potency test to differences in HA antigen glycosylation.
30355697	9	33	theme	radial	1264:1269	arg1	immunodiffusion					1271:1285	single radial immunodiffusion	1257:1285	the single radial immunodiffusion (SRID) potency test	1253:1305	We also examined the sensitivity of the single radial immunodiffusion (SRID) potency test to differences in HA antigen glycosylation.
30355697	12	34	theme	standard	1988:1995	arg1	B/Massachusetts/02/2012					2059:2081	B/Massachusetts/02/2012	2059:2081	B/Massachusetts/02/2012	2059:2081	Differences in glycosylation patterns, as dictated by the cell system used for expression, may impact these functions.IMPORTANCE In the present study, the glycosylation patterns of the 2014-2015 influenza vaccine season standard antigens A/California/07/2009 H1N1, A/Texas/50/2012 H3N2, and B/Massachusetts/02/2012 were revealed, and the sensitivity of the single radial immunodiffusion (SRID) potency test to glycosylation was tested.
30355697	12	34	theme	standard	1988:1995	arg1	H3N2					2049:2052	H3N2	2049:2052	H3N2	2049:2052	Differences in glycosylation patterns, as dictated by the cell system used for expression, may impact these functions.IMPORTANCE In the present study, the glycosylation patterns of the 2014-2015 influenza vaccine season standard antigens A/California/07/2009 H1N1, A/Texas/50/2012 H3N2, and B/Massachusetts/02/2012 were revealed, and the sensitivity of the single radial immunodiffusion (SRID) potency test to glycosylation was tested.
30355697	12	34	theme	standard	1988:1995	arg1	antigens					1997:2004	the 2014-2015 influenza vaccine season standard antigens	1949:2004	the 2014-2015 influenza vaccine season standard antigens A/California/07/2009 H1N1, A/Texas/50/2012 H3N2, and B/Massachusetts/02/2012	1949:2081	Differences in glycosylation patterns, as dictated by the cell system used for expression, may impact these functions.IMPORTANCE In the present study, the glycosylation patterns of the 2014-2015 influenza vaccine season standard antigens A/California/07/2009 H1N1, A/Texas/50/2012 H3N2, and B/Massachusetts/02/2012 were revealed, and the sensitivity of the single radial immunodiffusion (SRID) potency test to glycosylation was tested.
30355697	11	35	theme	lectin	1619:1624	arg1	regions					1638:1644	lectin interaction regions	1619:1644	lectin interaction regions	1619:1644	Mapping of glycosites with their occupying glycan to functional regions, including antigenic sites, lectin interaction regions, and fusion domains, was performed and has implications for immune processing, immune responses, and antigenic shielding.
30355697	14	36	from	differences	2471:2481	arg1	glycosylation					2486:2498	glycosylation	2486:2498	glycosylation	2486:2498	The SRID potency test used for vaccine release is not sensitive to differences in glycosylation under standard use conditions.
30355697	0	37	theme	Immune	98:103	arg1	Processing					105:114	Immune Processing	98:114	Immune Processing	98:114	N-Glycosylation of Seasonal Influenza Vaccine Hemagglutinins: Implication for Potency Testing and Immune Processing.
30355697	13	38	theme	glycosylation	2233:2245	arg1	composition					2252:2262	hemagglutinin glycosylation site composition	2219:2262	hemagglutinin glycosylation site composition	2219:2262	Differences in hemagglutinin glycosylation site composition and heterogeneity seen in antigens produced in different cell substrates suggest differences in processing and downstream immune responses.
30355697	10	39	theme	assay	1407:1411	arg1	procedures					1413:1422	standard assay procedures	1398:1422	standard assay procedures	1398:1422	Based on deglycosylation studies applied using standard assay procedures, the SRID assay was not sensitive to any HA antigen glycosylation status from any cell system.
30355697	13	40	from	differences	2345:2355	arg1	processing					2360:2369	processing	2360:2369	processing	2360:2369	Differences in hemagglutinin glycosylation site composition and heterogeneity seen in antigens produced in different cell substrates suggest differences in processing and downstream immune responses.
30355697	13	40	from	differences	2345:2355	arg1	responses					2393:2401	downstream immune responses	2375:2401	downstream immune responses	2375:2401	Differences in hemagglutinin glycosylation site composition and heterogeneity seen in antigens produced in different cell substrates suggest differences in processing and downstream immune responses.
30355697	4	41	from	use	753:755	arg1	testing					768:774	vaccine testing	760:774	vaccine testing	760:774	These reagents are supplied by the Center for Biologics Evaluation and Research (CBER) or the National Institute for Biological Standards and Control (NIBSC) for use in vaccine testing.
30355697	0	42	theme	Hemagglutinins	46:59	arg1	N-Glycosylation					0:14	N-Glycosylation	0:14	N-Glycosylation of Seasonal Influenza Vaccine Hemagglutinins: Implication for Potency Testing and Immune Processing.	0:115	N-Glycosylation of Seasonal Influenza Vaccine Hemagglutinins: Implication for Potency Testing and Immune Processing.
30355697	15	43	theme	immune	2686:2691	arg1	processing					2693:2702	immune processing	2686:2702	immune processing	2686:2702	This work reveals important differences in vaccine antigens and may point out areas where improvements may be made concerning vaccine antigen preparation, immune processing, and testing.
30355697	1	44	theme	A	261:261	arg1	strains					199:205	three or four virus strains	179:205	three or four virus strains for inclusion in the annual influenza vaccine: a type A:H1N1 virus, a type A:H3N2 virus, and one or two type B viruses	179:324	Prior to each annual flu season, health authorities recommend three or four virus strains for inclusion in the annual influenza vaccine: a type A:H1N1 virus, a type A:H3N2 virus, and one or two type B viruses.
30355697	1	44	theme	A	261:261	arg1	virus					268:272	a type A:H1N1 virus	254:272	a type A:H1N1 virus	254:272	Prior to each annual flu season, health authorities recommend three or four virus strains for inclusion in the annual influenza vaccine: a type A:H1N1 virus, a type A:H3N2 virus, and one or two type B viruses.
30355697	10	45	theme	SRID	1429:1432	arg1	assay					1434:1438	the SRID assay	1425:1438	the SRID assay	1425:1438	Based on deglycosylation studies applied using standard assay procedures, the SRID assay was not sensitive to any HA antigen glycosylation status from any cell system.
30355697	10	45	theme	SRID	1429:1432	arg1	sensitive					1448:1456	sensitive	1448:1456	sensitive	1448:1456	Based on deglycosylation studies applied using standard assay procedures, the SRID assay was not sensitive to any HA antigen glycosylation status from any cell system.
30355697	12	46	theme	potency	2162:2168	arg1	SRID					2156:2159	SRID	2156:2159	SRID	2156:2159	Differences in glycosylation patterns, as dictated by the cell system used for expression, may impact these functions.IMPORTANCE In the present study, the glycosylation patterns of the 2014-2015 influenza vaccine season standard antigens A/California/07/2009 H1N1, A/Texas/50/2012 H3N2, and B/Massachusetts/02/2012 were revealed, and the sensitivity of the single radial immunodiffusion (SRID) potency test to glycosylation was tested.
30355697	12	46	theme	potency	2162:2168	arg1	test					2170:2173	the single radial immunodiffusion (SRID) potency test	2121:2173	the single radial immunodiffusion (SRID) potency test	2121:2173	Differences in glycosylation patterns, as dictated by the cell system used for expression, may impact these functions.IMPORTANCE In the present study, the glycosylation patterns of the 2014-2015 influenza vaccine season standard antigens A/California/07/2009 H1N1, A/Texas/50/2012 H3N2, and B/Massachusetts/02/2012 were revealed, and the sensitivity of the single radial immunodiffusion (SRID) potency test to glycosylation was tested.
30355697	0	47	theme	Seasonal	19:26	arg1	Hemagglutinins					46:59	Seasonal Influenza Vaccine Hemagglutinins	19:59	Seasonal Influenza Vaccine Hemagglutinins	19:59	N-Glycosylation of Seasonal Influenza Vaccine Hemagglutinins: Implication for Potency Testing and Immune Processing.
30355697	1	48	theme	H1N1	263:266	arg1	strains					199:205	three or four virus strains	179:205	three or four virus strains for inclusion in the annual influenza vaccine: a type A:H1N1 virus, a type A:H3N2 virus, and one or two type B viruses	179:324	Prior to each annual flu season, health authorities recommend three or four virus strains for inclusion in the annual influenza vaccine: a type A:H1N1 virus, a type A:H3N2 virus, and one or two type B viruses.
30355697	1	48	theme	H1N1	263:266	arg1	virus					268:272	a type A:H1N1 virus	254:272	a type A:H1N1 virus	254:272	Prior to each annual flu season, health authorities recommend three or four virus strains for inclusion in the annual influenza vaccine: a type A:H1N1 virus, a type A:H3N2 virus, and one or two type B viruses.
30355697	12	49	theme	glycosylation	1783:1795	arg1	patterns					1797:1804	glycosylation patterns	1783:1804	glycosylation patterns	1783:1804	Differences in glycosylation patterns, as dictated by the cell system used for expression, may impact these functions.IMPORTANCE In the present study, the glycosylation patterns of the 2014-2015 influenza vaccine season standard antigens A/California/07/2009 H1N1, A/Texas/50/2012 H3N2, and B/Massachusetts/02/2012 were revealed, and the sensitivity of the single radial immunodiffusion (SRID) potency test to glycosylation was tested.
30355697	12	50	dep	antigens	1997:2004	arg1	A/Texas/50/2012					2033:2047	A/Texas/50/2012	2033:2047	A/Texas/50/2012	2033:2047	Differences in glycosylation patterns, as dictated by the cell system used for expression, may impact these functions.IMPORTANCE In the present study, the glycosylation patterns of the 2014-2015 influenza vaccine season standard antigens A/California/07/2009 H1N1, A/Texas/50/2012 H3N2, and B/Massachusetts/02/2012 were revealed, and the sensitivity of the single radial immunodiffusion (SRID) potency test to glycosylation was tested.
30355697	12	50	dep	antigens	1997:2004	arg1	antigens					1997:2004	the 2014-2015 influenza vaccine season standard antigens	1949:2004	the 2014-2015 influenza vaccine season standard antigens A/California/07/2009 H1N1, A/Texas/50/2012 H3N2, and B/Massachusetts/02/2012	1949:2081	Differences in glycosylation patterns, as dictated by the cell system used for expression, may impact these functions.IMPORTANCE In the present study, the glycosylation patterns of the 2014-2015 influenza vaccine season standard antigens A/California/07/2009 H1N1, A/Texas/50/2012 H3N2, and B/Massachusetts/02/2012 were revealed, and the sensitivity of the single radial immunodiffusion (SRID) potency test to glycosylation was tested.
30355697	12	50	dep	antigens	1997:2004	arg1	H3N2					2049:2052	H3N2	2049:2052	H3N2	2049:2052	Differences in glycosylation patterns, as dictated by the cell system used for expression, may impact these functions.IMPORTANCE In the present study, the glycosylation patterns of the 2014-2015 influenza vaccine season standard antigens A/California/07/2009 H1N1, A/Texas/50/2012 H3N2, and B/Massachusetts/02/2012 were revealed, and the sensitivity of the single radial immunodiffusion (SRID) potency test to glycosylation was tested.
30355697	12	50	dep	antigens	1997:2004	arg1	B/Massachusetts/02/2012					2059:2081	B/Massachusetts/02/2012	2059:2081	B/Massachusetts/02/2012	2059:2081	Differences in glycosylation patterns, as dictated by the cell system used for expression, may impact these functions.IMPORTANCE In the present study, the glycosylation patterns of the 2014-2015 influenza vaccine season standard antigens A/California/07/2009 H1N1, A/Texas/50/2012 H3N2, and B/Massachusetts/02/2012 were revealed, and the sensitivity of the single radial immunodiffusion (SRID) potency test to glycosylation was tested.
30355697	11	51	theme	interaction	1626:1636	arg1	regions					1638:1644	lectin interaction regions	1619:1644	lectin interaction regions	1619:1644	Mapping of glycosites with their occupying glycan to functional regions, including antigenic sites, lectin interaction regions, and fusion domains, was performed and has implications for immune processing, immune responses, and antigenic shielding.
30355697	12	52	theme	single	2125:2130	arg1	immunodiffusion					2139:2153	the single radial immunodiffusion	2121:2153	the single radial immunodiffusion (SRID) potency test	2121:2173	Differences in glycosylation patterns, as dictated by the cell system used for expression, may impact these functions.IMPORTANCE In the present study, the glycosylation patterns of the 2014-2015 influenza vaccine season standard antigens A/California/07/2009 H1N1, A/Texas/50/2012 H3N2, and B/Massachusetts/02/2012 were revealed, and the sensitivity of the single radial immunodiffusion (SRID) potency test to glycosylation was tested.
30355697	0	53	theme	Vaccine	38:44	arg1	Hemagglutinins					46:59	Seasonal Influenza Vaccine Hemagglutinins	19:59	Seasonal Influenza Vaccine Hemagglutinins	19:59	N-Glycosylation of Seasonal Influenza Vaccine Hemagglutinins: Implication for Potency Testing and Immune Processing.
30355697	7	54	theme	influenza	1026:1034	arg1	season					1036:1041	the 2014-2015 influenza season	1012:1041	the 2014-2015 influenza season	1012:1041	The reference antigens studied were used in the 2014-2015 influenza season and included A/California/07/2009 H1N1, A/Texas/50/2012 H3N2, and B/Massachusetts/02/2012.
30355697	3	55	theme	vaccine	566:572	arg1	testing					582:588	influenza vaccine potency testing	556:588	influenza vaccine potency testing	556:588	Here we examine the glycosylation patterns of seven reference antigens containing HA used in influenza vaccine potency testing.
30355697	12	56	theme	immunodiffusion	2139:2153	arg1	SRID					2156:2159	SRID	2156:2159	SRID	2156:2159	Differences in glycosylation patterns, as dictated by the cell system used for expression, may impact these functions.IMPORTANCE In the present study, the glycosylation patterns of the 2014-2015 influenza vaccine season standard antigens A/California/07/2009 H1N1, A/Texas/50/2012 H3N2, and B/Massachusetts/02/2012 were revealed, and the sensitivity of the single radial immunodiffusion (SRID) potency test to glycosylation was tested.
30355697	12	56	theme	immunodiffusion	2139:2153	arg1	test					2170:2173	the single radial immunodiffusion (SRID) potency test	2121:2173	the single radial immunodiffusion (SRID) potency test	2121:2173	Differences in glycosylation patterns, as dictated by the cell system used for expression, may impact these functions.IMPORTANCE In the present study, the glycosylation patterns of the 2014-2015 influenza vaccine season standard antigens A/California/07/2009 H1N1, A/Texas/50/2012 H3N2, and B/Massachusetts/02/2012 were revealed, and the sensitivity of the single radial immunodiffusion (SRID) potency test to glycosylation was tested.
30355697	1	57	theme	type	277:280	arg1	virus					289:293	a type A:H3N2 virus	275:293	a type A:H3N2 virus	275:293	Prior to each annual flu season, health authorities recommend three or four virus strains for inclusion in the annual influenza vaccine: a type A:H1N1 virus, a type A:H3N2 virus, and one or two type B viruses.
30355697	1	57	theme	type	277:280	arg1	strains					199:205	three or four virus strains	179:205	three or four virus strains for inclusion in the annual influenza vaccine: a type A:H1N1 virus, a type A:H3N2 virus, and one or two type B viruses	179:324	Prior to each annual flu season, health authorities recommend three or four virus strains for inclusion in the annual influenza vaccine: a type A:H1N1 virus, a type A:H3N2 virus, and one or two type B viruses.
30355697	14	58	theme	standard	2506:2513	arg1	conditions					2519:2528	standard use conditions	2506:2528	standard use conditions	2506:2528	The SRID potency test used for vaccine release is not sensitive to differences in glycosylation under standard use conditions.
30355697	13	59	theme	downstream	2375:2384	arg1	responses					2393:2401	downstream immune responses	2375:2401	downstream immune responses	2375:2401	Differences in hemagglutinin glycosylation site composition and heterogeneity seen in antigens produced in different cell substrates suggest differences in processing and downstream immune responses.
30355697	11	60	theme	fusion	1651:1656	arg1	domains					1658:1664	fusion domains	1651:1664	fusion domains	1651:1664	Mapping of glycosites with their occupying glycan to functional regions, including antigenic sites, lectin interaction regions, and fusion domains, was performed and has implications for immune processing, immune responses, and antigenic shielding.
30355697	4	61	theme	Biological	708:717	arg1	Standards					719:727	Biological Standards	708:727	Biological Standards	708:727	These reagents are supplied by the Center for Biologics Evaluation and Research (CBER) or the National Institute for Biological Standards and Control (NIBSC) for use in vaccine testing.
30355697	14	62	theme	SRID	2408:2411	arg1	sensitive					2458:2466	sensitive	2458:2466	sensitive	2458:2466	The SRID potency test used for vaccine release is not sensitive to differences in glycosylation under standard use conditions.
30355697	14	62	theme	SRID	2408:2411	arg1	test					2421:2424	The SRID potency test	2404:2424	The SRID potency test used for vaccine release	2404:2449	The SRID potency test used for vaccine release is not sensitive to differences in glycosylation under standard use conditions.
30355697	9	63	theme	test	1302:1305	arg1	sensitivity					1238:1248	the sensitivity	1234:1248	the sensitivity of the single radial immunodiffusion (SRID) potency test to differences in HA antigen glycosylation	1234:1348	We also examined the sensitivity of the single radial immunodiffusion (SRID) potency test to differences in HA antigen glycosylation.
30355697	3	64	contain	containing	534:543	arg2	HA					545:546	HA	545:546	HA used in influenza vaccine potency testing	545:588	Here we examine the glycosylation patterns of seven reference antigens containing HA used in influenza vaccine potency testing.
30355697	3	64	contain	containing	534:543	arg1	antigens					525:532	seven reference antigens	509:532	seven reference antigens containing HA used in influenza vaccine potency testing	509:588	Here we examine the glycosylation patterns of seven reference antigens containing HA used in influenza vaccine potency testing.
30355697	10	65	theme	antigen	1468:1474	arg1	status					1490:1495	any HA antigen glycosylation status	1461:1495	any HA antigen glycosylation status from any cell system	1461:1516	Based on deglycosylation studies applied using standard assay procedures, the SRID assay was not sensitive to any HA antigen glycosylation status from any cell system.
30355697	15	66	theme	vaccine	2574:2580	arg1	antigens					2582:2589	vaccine antigens	2574:2589	vaccine antigens	2574:2589	This work reveals important differences in vaccine antigens and may point out areas where improvements may be made concerning vaccine antigen preparation, immune processing, and testing.
30355697	1	67	theme	annual	228:233	arg1	vaccine					245:251	the annual influenza vaccine	224:251	the annual influenza vaccine	224:251	Prior to each annual flu season, health authorities recommend three or four virus strains for inclusion in the annual influenza vaccine: a type A:H1N1 virus, a type A:H3N2 virus, and one or two type B viruses.
30355697	10	68	from	system	1511:1516	arg1	status					1490:1495	any HA antigen glycosylation status	1461:1495	any HA antigen glycosylation status from any cell system	1461:1516	Based on deglycosylation studies applied using standard assay procedures, the SRID assay was not sensitive to any HA antigen glycosylation status from any cell system.
30355697	0	69	dep	N-Glycosylation	0:14	arg1	Implication					62:72	Implication	62:72	N-Glycosylation of Seasonal Influenza Vaccine Hemagglutinins: Implication for Potency Testing and Immune Processing.	0:115	N-Glycosylation of Seasonal Influenza Vaccine Hemagglutinins: Implication for Potency Testing and Immune Processing.
30355697	11	70	theme	functional	1572:1581	arg1	domains					1658:1664	fusion domains	1651:1664	fusion domains	1651:1664	Mapping of glycosites with their occupying glycan to functional regions, including antigenic sites, lectin interaction regions, and fusion domains, was performed and has implications for immune processing, immune responses, and antigenic shielding.
30355697	11	70	theme	functional	1572:1581	arg1	regions					1638:1644	lectin interaction regions	1619:1644	lectin interaction regions	1619:1644	Mapping of glycosites with their occupying glycan to functional regions, including antigenic sites, lectin interaction regions, and fusion domains, was performed and has implications for immune processing, immune responses, and antigenic shielding.
30355697	11	70	theme	functional	1572:1581	arg1	regions					1583:1589	functional regions	1572:1589	functional regions	1572:1589	Mapping of glycosites with their occupying glycan to functional regions, including antigenic sites, lectin interaction regions, and fusion domains, was performed and has implications for immune processing, immune responses, and antigenic shielding.
30355697	11	70	theme	functional	1572:1581	arg1	sites					1612:1616	antigenic sites	1602:1616	antigenic sites	1602:1616	Mapping of glycosites with their occupying glycan to functional regions, including antigenic sites, lectin interaction regions, and fusion domains, was performed and has implications for immune processing, immune responses, and antigenic shielding.
30355697	11	71	theme	glycosites	1530:1539	arg1	Mapping					1519:1525	Mapping	1519:1525	Mapping of glycosites with their occupying glycan to functional regions, including antigenic sites, lectin interaction regions, and fusion domains,	1519:1665	Mapping of glycosites with their occupying glycan to functional regions, including antigenic sites, lectin interaction regions, and fusion domains, was performed and has implications for immune processing, immune responses, and antigenic shielding.
30355697	9	72	theme	HA	1325:1326	arg1	glycosylation					1336:1348	HA antigen glycosylation	1325:1348	HA antigen glycosylation	1325:1348	We also examined the sensitivity of the single radial immunodiffusion (SRID) potency test to differences in HA antigen glycosylation.
30355697	11	73	contain	has	1685:1687	arg2	implications					1689:1700	implications	1689:1700	implications for immune processing, immune responses, and antigenic shielding	1689:1765	Mapping of glycosites with their occupying glycan to functional regions, including antigenic sites, lectin interaction regions, and fusion domains, was performed and has implications for immune processing, immune responses, and antigenic shielding.
30355697	11	73	contain	has	1685:1687	arg1	Mapping					1519:1525	Mapping	1519:1525	Mapping of glycosites with their occupying glycan to functional regions, including antigenic sites, lectin interaction regions, and fusion domains,	1519:1665	Mapping of glycosites with their occupying glycan to functional regions, including antigenic sites, lectin interaction regions, and fusion domains, was performed and has implications for immune processing, immune responses, and antigenic shielding.
30355697	14	74	theme	use	2515:2517	arg1	conditions					2519:2528	standard use conditions	2506:2528	standard use conditions	2506:2528	The SRID potency test used for vaccine release is not sensitive to differences in glycosylation under standard use conditions.
30355697	11	75	theme	antigenic	1602:1610	arg1	sites					1612:1616	antigenic sites	1602:1616	antigenic sites	1602:1616	Mapping of glycosites with their occupying glycan to functional regions, including antigenic sites, lectin interaction regions, and fusion domains, was performed and has implications for immune processing, immune responses, and antigenic shielding.
30355697	14	76	theme	vaccine	2435:2441	arg1	release					2443:2449	vaccine release	2435:2449	vaccine release	2435:2449	The SRID potency test used for vaccine release is not sensitive to differences in glycosylation under standard use conditions.
30355697	8	77	theme	Released	1134:1141	arg1	patterns					1193:1200	Released glycan and HA-specific glycopeptide glycosylation patterns	1134:1200	Released glycan and HA-specific glycopeptide glycosylation patterns	1134:1200	Released glycan and HA-specific glycopeptide glycosylation patterns were examined.
30355697	3	78	theme	glycosylation	483:495	arg1	patterns					497:504	the glycosylation patterns	479:504	the glycosylation patterns of seven reference antigens containing HA used in influenza vaccine potency testing	479:588	Here we examine the glycosylation patterns of seven reference antigens containing HA used in influenza vaccine potency testing.
30355697	11	79	gly	glycosites	1530:1539	arg2	glycosites					1530:1539	glycosites	1530:1539	glycosites with their occupying glycan to functional regions, including antigenic sites, lectin interaction regions, and fusion domains,	1530:1665	Mapping of glycosites with their occupying glycan to functional regions, including antigenic sites, lectin interaction regions, and fusion domains, was performed and has implications for immune processing, immune responses, and antigenic shielding.
30355697	12	80	dep	impact	1863:1868	arg1	dictated					1810:1817	dictated	1810:1817	dictated by the cell system used for expression	1810:1856	Differences in glycosylation patterns, as dictated by the cell system used for expression, may impact these functions.IMPORTANCE In the present study, the glycosylation patterns of the 2014-2015 influenza vaccine season standard antigens A/California/07/2009 H1N1, A/Texas/50/2012 H3N2, and B/Massachusetts/02/2012 were revealed, and the sensitivity of the single radial immunodiffusion (SRID) potency test to glycosylation was tested.
30355697	11	81	theme	immune	1725:1730	arg1	responses					1732:1740	immune responses	1725:1740	immune responses	1725:1740	Mapping of glycosites with their occupying glycan to functional regions, including antigenic sites, lectin interaction regions, and fusion domains, was performed and has implications for immune processing, immune responses, and antigenic shielding.
30355697	1	82	theme	virus	193:197	arg1	virus					289:293	a type A:H3N2 virus	275:293	a type A:H3N2 virus	275:293	Prior to each annual flu season, health authorities recommend three or four virus strains for inclusion in the annual influenza vaccine: a type A:H1N1 virus, a type A:H3N2 virus, and one or two type B viruses.
30355697	1	82	theme	virus	193:197	arg1	strains					199:205	three or four virus strains	179:205	three or four virus strains for inclusion in the annual influenza vaccine: a type A:H1N1 virus, a type A:H3N2 virus, and one or two type B viruses	179:324	Prior to each annual flu season, health authorities recommend three or four virus strains for inclusion in the annual influenza vaccine: a type A:H1N1 virus, a type A:H3N2 virus, and one or two type B viruses.
30355697	1	82	theme	virus	193:197	arg1	virus					268:272	a type A:H1N1 virus	254:272	a type A:H1N1 virus	254:272	Prior to each annual flu season, health authorities recommend three or four virus strains for inclusion in the annual influenza vaccine: a type A:H1N1 virus, a type A:H3N2 virus, and one or two type B viruses.
30355697	8	83	theme	glycopeptide	1166:1177	arg1	patterns					1193:1200	Released glycan and HA-specific glycopeptide glycosylation patterns	1134:1200	Released glycan and HA-specific glycopeptide glycosylation patterns	1134:1200	Released glycan and HA-specific glycopeptide glycosylation patterns were examined.
30355697	1	84	theme	B	316:316	arg1	viruses					318:324	one or two type B viruses	300:324	one or two type B viruses	300:324	Prior to each annual flu season, health authorities recommend three or four virus strains for inclusion in the annual influenza vaccine: a type A:H1N1 virus, a type A:H3N2 virus, and one or two type B viruses.
30355697	3	85	theme	reference	515:523	arg1	antigens					525:532	seven reference antigens	509:532	seven reference antigens containing HA used in influenza vaccine potency testing	509:588	Here we examine the glycosylation patterns of seven reference antigens containing HA used in influenza vaccine potency testing.
30355697	1	86	theme	A	282:282	arg1	virus					289:293	a type A:H3N2 virus	275:293	a type A:H3N2 virus	275:293	Prior to each annual flu season, health authorities recommend three or four virus strains for inclusion in the annual influenza vaccine: a type A:H1N1 virus, a type A:H3N2 virus, and one or two type B viruses.
30355697	1	86	theme	A	282:282	arg1	strains					199:205	three or four virus strains	179:205	three or four virus strains for inclusion in the annual influenza vaccine: a type A:H1N1 virus, a type A:H3N2 virus, and one or two type B viruses	179:324	Prior to each annual flu season, health authorities recommend three or four virus strains for inclusion in the annual influenza vaccine: a type A:H1N1 virus, a type A:H3N2 virus, and one or two type B viruses.
30355697	11	87	theme	antigenic	1747:1755	arg1	shielding					1757:1765	antigenic shielding	1747:1765	antigenic shielding	1747:1765	Mapping of glycosites with their occupying glycan to functional regions, including antigenic sites, lectin interaction regions, and fusion domains, was performed and has implications for immune processing, immune responses, and antigenic shielding.
30355697	1	88	from	inclusion	211:219	arg1	vaccine					245:251	the annual influenza vaccine	224:251	the annual influenza vaccine	224:251	Prior to each annual flu season, health authorities recommend three or four virus strains for inclusion in the annual influenza vaccine: a type A:H1N1 virus, a type A:H3N2 virus, and one or two type B viruses.
30355697	5	89	theme	Madin-Darby	804:814	arg1	MDCK					831:834	MDCK	831:834	MDCK	831:834	Those produced in hen egg, Madin-Darby canine kidney (MDCK), and insect (Sf9) expression systems were examined.
30355697	5	89	theme	Madin-Darby	804:814	arg1	kidney					823:828	Madin-Darby canine kidney	804:828	Madin-Darby canine kidney (MDCK)	804:835	Those produced in hen egg, Madin-Darby canine kidney (MDCK), and insect (Sf9) expression systems were examined.
30355697	3	90	gly	glycosylation	483:495	arg1	antigens					525:532	seven reference antigens	509:532	seven reference antigens containing HA used in influenza vaccine potency testing	509:588	Here we examine the glycosylation patterns of seven reference antigens containing HA used in influenza vaccine potency testing.
30355697	13	91	theme	cell	2321:2324	arg1	substrates					2326:2335	different cell substrates	2311:2335	different cell substrates	2311:2335	Differences in hemagglutinin glycosylation site composition and heterogeneity seen in antigens produced in different cell substrates suggest differences in processing and downstream immune responses.
30355697	1	92	theme	annual	131:136	arg1	season					142:147	each annual flu season	126:147	each annual flu season	126:147	Prior to each annual flu season, health authorities recommend three or four virus strains for inclusion in the annual influenza vaccine: a type A:H1N1 virus, a type A:H3N2 virus, and one or two type B viruses.
30355697	0	93	theme	Potency	78:84	arg1	Testing					86:92	Potency Testing	78:92	Potency Testing	78:92	N-Glycosylation of Seasonal Influenza Vaccine Hemagglutinins: Implication for Potency Testing and Immune Processing.
30355697	2	94	theme	virus	428:432	arg1	hemagglutinin					443:455	hemagglutinin	443:455	hemagglutinin (HA)	443:460	Antigenic differences between strains are found in the glycosylation patterns of the major influenza virus antigen, hemagglutinin (HA).
30355697	2	94	theme	virus	428:432	arg1	antigen					434:440	the major influenza virus antigen	408:440	the major influenza virus antigen	408:440	Antigenic differences between strains are found in the glycosylation patterns of the major influenza virus antigen, hemagglutinin (HA).
30355697	13	95	gly	glycosylation	2233:2245	arg2	composition					2252:2262	hemagglutinin glycosylation site composition	2219:2262	hemagglutinin glycosylation site composition	2219:2262	Differences in hemagglutinin glycosylation site composition and heterogeneity seen in antigens produced in different cell substrates suggest differences in processing and downstream immune responses.
30355697	13	95	gly	glycosylation	2233:2245	arg2	site					2247:2250	hemagglutinin glycosylation site composition	2219:2262	hemagglutinin glycosylation site composition	2219:2262	Differences in hemagglutinin glycosylation site composition and heterogeneity seen in antigens produced in different cell substrates suggest differences in processing and downstream immune responses.
30355697	12	96	theme	influenza	1963:1971	arg1	season					1981:1986	the 2014-2015 influenza vaccine season	1949:1986	the 2014-2015 influenza vaccine season standard antigens A/California/07/2009 H1N1, A/Texas/50/2012 H3N2, and B/Massachusetts/02/2012	1949:2081	Differences in glycosylation patterns, as dictated by the cell system used for expression, may impact these functions.IMPORTANCE In the present study, the glycosylation patterns of the 2014-2015 influenza vaccine season standard antigens A/California/07/2009 H1N1, A/Texas/50/2012 H3N2, and B/Massachusetts/02/2012 were revealed, and the sensitivity of the single radial immunodiffusion (SRID) potency test to glycosylation was tested.
30355697	15	97	theme	antigen	2665:2671	arg1	preparation					2673:2683	vaccine antigen preparation	2657:2683	vaccine antigen preparation	2657:2683	This work reveals important differences in vaccine antigens and may point out areas where improvements may be made concerning vaccine antigen preparation, immune processing, and testing.
30355697	1	98	theme	H3N2	284:287	arg1	virus					289:293	a type A:H3N2 virus	275:293	a type A:H3N2 virus	275:293	Prior to each annual flu season, health authorities recommend three or four virus strains for inclusion in the annual influenza vaccine: a type A:H1N1 virus, a type A:H3N2 virus, and one or two type B viruses.
30355697	1	98	theme	H3N2	284:287	arg1	strains					199:205	three or four virus strains	179:205	three or four virus strains for inclusion in the annual influenza vaccine: a type A:H1N1 virus, a type A:H3N2 virus, and one or two type B viruses	179:324	Prior to each annual flu season, health authorities recommend three or four virus strains for inclusion in the annual influenza vaccine: a type A:H1N1 virus, a type A:H3N2 virus, and one or two type B viruses.
30355697	1	99	theme	health	150:155	arg1	authorities					157:167	health authorities	150:167	health authorities	150:167	Prior to each annual flu season, health authorities recommend three or four virus strains for inclusion in the annual influenza vaccine: a type A:H1N1 virus, a type A:H3N2 virus, and one or two type B viruses.
30355697	2	100	theme	major	412:416	arg1	hemagglutinin					443:455	hemagglutinin	443:455	hemagglutinin (HA)	443:460	Antigenic differences between strains are found in the glycosylation patterns of the major influenza virus antigen, hemagglutinin (HA).
30355697	2	100	theme	major	412:416	arg1	antigen					434:440	the major influenza virus antigen	408:440	the major influenza virus antigen	408:440	Antigenic differences between strains are found in the glycosylation patterns of the major influenza virus antigen, hemagglutinin (HA).
30355697	10	101	theme	deglycosylation	1360:1374	arg1	studies					1376:1382	deglycosylation studies	1360:1382	deglycosylation studies applied using standard assay procedures	1360:1422	Based on deglycosylation studies applied using standard assay procedures, the SRID assay was not sensitive to any HA antigen glycosylation status from any cell system.
30355697	12	102	theme	season	1981:1986	arg1	B/Massachusetts/02/2012					2059:2081	B/Massachusetts/02/2012	2059:2081	B/Massachusetts/02/2012	2059:2081	Differences in glycosylation patterns, as dictated by the cell system used for expression, may impact these functions.IMPORTANCE In the present study, the glycosylation patterns of the 2014-2015 influenza vaccine season standard antigens A/California/07/2009 H1N1, A/Texas/50/2012 H3N2, and B/Massachusetts/02/2012 were revealed, and the sensitivity of the single radial immunodiffusion (SRID) potency test to glycosylation was tested.
30355697	12	102	theme	season	1981:1986	arg1	H3N2					2049:2052	H3N2	2049:2052	H3N2	2049:2052	Differences in glycosylation patterns, as dictated by the cell system used for expression, may impact these functions.IMPORTANCE In the present study, the glycosylation patterns of the 2014-2015 influenza vaccine season standard antigens A/California/07/2009 H1N1, A/Texas/50/2012 H3N2, and B/Massachusetts/02/2012 were revealed, and the sensitivity of the single radial immunodiffusion (SRID) potency test to glycosylation was tested.
30355697	12	102	theme	season	1981:1986	arg1	antigens					1997:2004	the 2014-2015 influenza vaccine season standard antigens	1949:2004	the 2014-2015 influenza vaccine season standard antigens A/California/07/2009 H1N1, A/Texas/50/2012 H3N2, and B/Massachusetts/02/2012	1949:2081	Differences in glycosylation patterns, as dictated by the cell system used for expression, may impact these functions.IMPORTANCE In the present study, the glycosylation patterns of the 2014-2015 influenza vaccine season standard antigens A/California/07/2009 H1N1, A/Texas/50/2012 H3N2, and B/Massachusetts/02/2012 were revealed, and the sensitivity of the single radial immunodiffusion (SRID) potency test to glycosylation was tested.
30355697	7	103	theme	A/Texas/50/2012	1083:1097	arg1	H3N2					1099:1102	A/Texas/50/2012 H3N2	1083:1102	A/Texas/50/2012 H3N2	1083:1102	The reference antigens studied were used in the 2014-2015 influenza season and included A/California/07/2009 H1N1, A/Texas/50/2012 H3N2, and B/Massachusetts/02/2012.
30355697	5	104	theme	insect	842:847	arg1	systems					866:872	insect (Sf9) expression systems	842:872	insect (Sf9) expression systems	842:872	Those produced in hen egg, Madin-Darby canine kidney (MDCK), and insect (Sf9) expression systems were examined.
30355697	12	105	theme	test	2170:2173	arg1	sensitivity					2106:2116	the sensitivity	2102:2116	the sensitivity of the single radial immunodiffusion (SRID) potency test to glycosylation	2102:2190	Differences in glycosylation patterns, as dictated by the cell system used for expression, may impact these functions.IMPORTANCE In the present study, the glycosylation patterns of the 2014-2015 influenza vaccine season standard antigens A/California/07/2009 H1N1, A/Texas/50/2012 H3N2, and B/Massachusetts/02/2012 were revealed, and the sensitivity of the single radial immunodiffusion (SRID) potency test to glycosylation was tested.
30355697	5	106	theme	Sf9	850:852	arg1	systems					866:872	insect (Sf9) expression systems	842:872	insect (Sf9) expression systems	842:872	Those produced in hen egg, Madin-Darby canine kidney (MDCK), and insect (Sf9) expression systems were examined.
30355697	6	107	theme	commercial	947:956	arg1	vaccines					958:965	commercial vaccines	947:965	commercial vaccines	947:965	They are closely related or identical to antigens used in commercial vaccines.
30355697	10	108	theme	standard	1398:1405	arg1	procedures					1413:1422	standard assay procedures	1398:1422	standard assay procedures	1398:1422	Based on deglycosylation studies applied using standard assay procedures, the SRID assay was not sensitive to any HA antigen glycosylation status from any cell system.
30355697	4	109	theme	National	685:692	arg1	Institute					694:702	the National Institute	681:702	the National Institute for Biological Standards and Control (NIBSC) for use in vaccine testing	681:774	These reagents are supplied by the Center for Biologics Evaluation and Research (CBER) or the National Institute for Biological Standards and Control (NIBSC) for use in vaccine testing.
30355697	12	110	theme	cell	1826:1829	arg1	system					1831:1836	the cell system	1822:1836	the cell system used for expression	1822:1856	Differences in glycosylation patterns, as dictated by the cell system used for expression, may impact these functions.IMPORTANCE In the present study, the glycosylation patterns of the 2014-2015 influenza vaccine season standard antigens A/California/07/2009 H1N1, A/Texas/50/2012 H3N2, and B/Massachusetts/02/2012 were revealed, and the sensitivity of the single radial immunodiffusion (SRID) potency test to glycosylation was tested.
30355697	13	111	theme	immune	2386:2391	arg1	responses					2393:2401	downstream immune responses	2375:2401	downstream immune responses	2375:2401	Differences in hemagglutinin glycosylation site composition and heterogeneity seen in antigens produced in different cell substrates suggest differences in processing and downstream immune responses.
30355697	0	112	theme	Influenza	28:36	arg1	Hemagglutinins					46:59	Seasonal Influenza Vaccine Hemagglutinins	19:59	Seasonal Influenza Vaccine Hemagglutinins	19:59	N-Glycosylation of Seasonal Influenza Vaccine Hemagglutinins: Implication for Potency Testing and Immune Processing.
30355697	9	113	theme	single	1257:1262	arg1	SRID					1288:1291	SRID	1288:1291	SRID	1288:1291	We also examined the sensitivity of the single radial immunodiffusion (SRID) potency test to differences in HA antigen glycosylation.
30355697	9	113	theme	single	1257:1262	arg1	immunodiffusion					1271:1285	single radial immunodiffusion	1257:1285	the single radial immunodiffusion (SRID) potency test	1253:1305	We also examined the sensitivity of the single radial immunodiffusion (SRID) potency test to differences in HA antigen glycosylation.
30355697	3	114	theme	potency	574:580	arg1	testing					582:588	influenza vaccine potency testing	556:588	influenza vaccine potency testing	556:588	Here we examine the glycosylation patterns of seven reference antigens containing HA used in influenza vaccine potency testing.
30355697	7	115	theme	2014-2015	1016:1024	arg1	season					1036:1041	the 2014-2015 influenza season	1012:1041	the 2014-2015 influenza season	1012:1041	The reference antigens studied were used in the 2014-2015 influenza season and included A/California/07/2009 H1N1, A/Texas/50/2012 H3N2, and B/Massachusetts/02/2012.
30355697	9	116	theme	immunodiffusion	1271:1285	arg1	test					1302:1305	the single radial immunodiffusion (SRID) potency test	1253:1305	the single radial immunodiffusion (SRID) potency test	1253:1305	We also examined the sensitivity of the single radial immunodiffusion (SRID) potency test to differences in HA antigen glycosylation.
30355697	0	117	gly	N-Glycosylation	0:14	arg1	Hemagglutinins					46:59	Seasonal Influenza Vaccine Hemagglutinins	19:59	Seasonal Influenza Vaccine Hemagglutinins	19:59	N-Glycosylation of Seasonal Influenza Vaccine Hemagglutinins: Implication for Potency Testing and Immune Processing.
30355697	3	118	theme	influenza	556:564	arg1	testing					582:588	influenza vaccine potency testing	556:588	influenza vaccine potency testing	556:588	Here we examine the glycosylation patterns of seven reference antigens containing HA used in influenza vaccine potency testing.
30355697	2	119	theme	Antigenic	327:335	arg1	differences					337:347	Antigenic differences	327:347	Antigenic differences between strains	327:363	Antigenic differences between strains are found in the glycosylation patterns of the major influenza virus antigen, hemagglutinin (HA).
30355697	9	120	from	differences	1310:1320	arg1	glycosylation					1336:1348	HA antigen glycosylation	1325:1348	HA antigen glycosylation	1325:1348	We also examined the sensitivity of the single radial immunodiffusion (SRID) potency test to differences in HA antigen glycosylation.
30355697	12	121	from	functions.IMPORTANCE	1876:1895	arg1	study					1912:1916	the present study	1900:1916	the present study	1900:1916	Differences in glycosylation patterns, as dictated by the cell system used for expression, may impact these functions.IMPORTANCE In the present study, the glycosylation patterns of the 2014-2015 influenza vaccine season standard antigens A/California/07/2009 H1N1, A/Texas/50/2012 H3N2, and B/Massachusetts/02/2012 were revealed, and the sensitivity of the single radial immunodiffusion (SRID) potency test to glycosylation was tested.
30355697	2	122	gly	glycosylation	382:394	arg1	hemagglutinin					443:455	hemagglutinin	443:455	hemagglutinin (HA)	443:460	Antigenic differences between strains are found in the glycosylation patterns of the major influenza virus antigen, hemagglutinin (HA).
30355697	2	122	gly	glycosylation	382:394	arg1	antigen					434:440	the major influenza virus antigen	408:440	the major influenza virus antigen	408:440	Antigenic differences between strains are found in the glycosylation patterns of the major influenza virus antigen, hemagglutinin (HA).
30355697	2	123	theme	glycosylation	382:394	arg1	patterns					396:403	the glycosylation patterns	378:403	the glycosylation patterns of the major influenza virus antigen, hemagglutinin (HA)	378:460	Antigenic differences between strains are found in the glycosylation patterns of the major influenza virus antigen, hemagglutinin (HA).
30355697	12	124	theme	radial	2132:2137	arg1	immunodiffusion					2139:2153	the single radial immunodiffusion	2121:2153	the single radial immunodiffusion (SRID) potency test	2121:2173	Differences in glycosylation patterns, as dictated by the cell system used for expression, may impact these functions.IMPORTANCE In the present study, the glycosylation patterns of the 2014-2015 influenza vaccine season standard antigens A/California/07/2009 H1N1, A/Texas/50/2012 H3N2, and B/Massachusetts/02/2012 were revealed, and the sensitivity of the single radial immunodiffusion (SRID) potency test to glycosylation was tested.
30355697	14	125	theme	potency	2413:2419	arg1	sensitive					2458:2466	sensitive	2458:2466	sensitive	2458:2466	The SRID potency test used for vaccine release is not sensitive to differences in glycosylation under standard use conditions.
30355697	14	125	theme	potency	2413:2419	arg1	test					2421:2424	The SRID potency test	2404:2424	The SRID potency test used for vaccine release	2404:2449	The SRID potency test used for vaccine release is not sensitive to differences in glycosylation under standard use conditions.
30355697	9	126	theme	potency	1294:1300	arg1	test					1302:1305	the single radial immunodiffusion (SRID) potency test	1253:1305	the single radial immunodiffusion (SRID) potency test	1253:1305	We also examined the sensitivity of the single radial immunodiffusion (SRID) potency test to differences in HA antigen glycosylation.
30355697	5	127	theme	expression	855:864	arg1	systems					866:872	insect (Sf9) expression systems	842:872	insect (Sf9) expression systems	842:872	Those produced in hen egg, Madin-Darby canine kidney (MDCK), and insect (Sf9) expression systems were examined.
30355697	15	128	from	differences	2559:2569	arg1	antigens					2582:2589	vaccine antigens	2574:2589	vaccine antigens	2574:2589	This work reveals important differences in vaccine antigens and may point out areas where improvements may be made concerning vaccine antigen preparation, immune processing, and testing.
30355697	10	129	theme	HA	1465:1466	arg1	status					1490:1495	any HA antigen glycosylation status	1461:1495	any HA antigen glycosylation status from any cell system	1461:1516	Based on deglycosylation studies applied using standard assay procedures, the SRID assay was not sensitive to any HA antigen glycosylation status from any cell system.
30355697	1	130	dep	strains	199:205	arg1	virus					289:293	a type A:H3N2 virus	275:293	a type A:H3N2 virus	275:293	Prior to each annual flu season, health authorities recommend three or four virus strains for inclusion in the annual influenza vaccine: a type A:H1N1 virus, a type A:H3N2 virus, and one or two type B viruses.
30355697	1	130	dep	strains	199:205	arg1	viruses					318:324	one or two type B viruses	300:324	one or two type B viruses	300:324	Prior to each annual flu season, health authorities recommend three or four virus strains for inclusion in the annual influenza vaccine: a type A:H1N1 virus, a type A:H3N2 virus, and one or two type B viruses.
30355697	1	130	dep	strains	199:205	arg1	virus					268:272	a type A:H1N1 virus	254:272	a type A:H1N1 virus	254:272	Prior to each annual flu season, health authorities recommend three or four virus strains for inclusion in the annual influenza vaccine: a type A:H1N1 virus, a type A:H3N2 virus, and one or two type B viruses.
30355697	1	130	dep	strains	199:205	arg1	strains					199:205	three or four virus strains	179:205	three or four virus strains for inclusion in the annual influenza vaccine: a type A:H1N1 virus, a type A:H3N2 virus, and one or two type B viruses	179:324	Prior to each annual flu season, health authorities recommend three or four virus strains for inclusion in the annual influenza vaccine: a type A:H1N1 virus, a type A:H3N2 virus, and one or two type B viruses.
30355697	15	131	theme	important	2549:2557	arg1	differences					2559:2569	important differences	2549:2569	important differences in vaccine antigens	2549:2589	This work reveals important differences in vaccine antigens and may point out areas where improvements may be made concerning vaccine antigen preparation, immune processing, and testing.
30355697	1	132	theme	influenza	235:243	arg1	vaccine					245:251	the annual influenza vaccine	224:251	the annual influenza vaccine	224:251	Prior to each annual flu season, health authorities recommend three or four virus strains for inclusion in the annual influenza vaccine: a type A:H1N1 virus, a type A:H3N2 virus, and one or two type B viruses.
31394179	3	0	theme	mushrooms	674:682	arg1	formation					647:655	formation	647:655	formation of the Sanghuang mushrooms	647:682	However, this mushroom is rarely available from the mulberry trees in the wild because of the rigorous conditions needed for formation of the Sanghuang mushrooms.
31394179	11	1	theme	higher	1844:1849	arg1	levels					1851:1856	significantly higher levels	1830:1856	significantly higher levels of polysaccharides, amino acids, and water-soluble nutraceuticals	1830:1922	The cultured mushrooms contained significantly higher levels of polysaccharides, amino acids, and water-soluble nutraceuticals, whereas flavones in the wild Sanghuang were significantly higher (P < 0.05).
31394179	1	2	theme	China	283:287	arg1	dynasty					272:278	Tang dynasty	267:278	Tang dynasty of China 1300 years ago	267:302	ETHNOPHARMACOLOGICAL RELEVANCE Description of the pharmacological activities of Sanghuang mushrooms (Inonotus Sanghuang) can be traced back to Tang dynasty of China 1300 years ago.
31394179	12	3	theme	16 mg/kg	2030:2037	arg1	dose					2011:2014	a dose	2009:2014	a dose of 8 mg/kg or 16 mg/kg to immunoregenerate the immunodeficient mice	2009:2082	Use of a dose of 8 mg/kg or 16 mg/kg to immunoregenerate the immunodeficient mice was comparable between the cultured and wild Sanghang based on analysis of hematological parameters and histological examination of the thymus and spleen in the treated mice.
31394179	4	4	theme	mushrooms	817:825	arg1	production					793:802	production	793:802	production of Sanghuang mushrooms	793:825	AIM OF THE STUDY This study aims to establish a practical protocol for culture, particularly for a bunch of production of Sanghuang mushrooms possibly to commercialize the cultured Sanghuang based on deep comparison of quality and pharmacological activities between the cultured and the wild Sanghuang.
31394179	11	5	theme	polysaccharides	1861:1875	arg1	levels					1851:1856	significantly higher levels	1830:1856	significantly higher levels of polysaccharides, amino acids, and water-soluble nutraceuticals	1830:1922	The cultured mushrooms contained significantly higher levels of polysaccharides, amino acids, and water-soluble nutraceuticals, whereas flavones in the wild Sanghuang were significantly higher (P < 0.05).
31394179	11	6	dep	higher	1983:1988	arg1	P < 0.05					1991:1998	P < 0.05	1991:1998	P < 0.05	1991:1998	The cultured mushrooms contained significantly higher levels of polysaccharides, amino acids, and water-soluble nutraceuticals, whereas flavones in the wild Sanghuang were significantly higher (P < 0.05).
31394179	1	7	theme	Sanghuang	204:212	arg1	mushrooms					214:222	Sanghuang mushrooms	204:222	Sanghuang mushrooms (Inonotus Sanghuang)	204:243	ETHNOPHARMACOLOGICAL RELEVANCE Description of the pharmacological activities of Sanghuang mushrooms (Inonotus Sanghuang) can be traced back to Tang dynasty of China 1300 years ago.
31394179	1	7	theme	Sanghuang	204:212	arg1	Sanghuang					234:242	Sanghuang	234:242	Sanghuang	234:242	ETHNOPHARMACOLOGICAL RELEVANCE Description of the pharmacological activities of Sanghuang mushrooms (Inonotus Sanghuang) can be traced back to Tang dynasty of China 1300 years ago.
31394179	9	8	theme	Sanghuang	1653:1661	arg1	culture					1642:1648	successful culture	1631:1648	successful culture of Sanghuang	1631:1661	RESULTS Maintenance of a temperature of 22-28 °C and a high relative humidity of 90-95%, and use of a high ratio (80%) of mulberry tree sticks in the medium were critical to successful culture of Sanghuang.
31394179	12	9	from	examination	2201:2211	arg1	mice					2253:2256	the treated mice	2241:2256	the treated mice	2241:2256	Use of a dose of 8 mg/kg or 16 mg/kg to immunoregenerate the immunodeficient mice was comparable between the cultured and wild Sanghang based on analysis of hematological parameters and histological examination of the thymus and spleen in the treated mice.
31394179	9	10	theme	temperature	1482:1492	arg1	Maintenance					1465:1475	Maintenance	1465:1475	Maintenance of a temperature of 22-28 °C and a high relative humidity of 90-95%	1465:1543	RESULTS Maintenance of a temperature of 22-28 °C and a high relative humidity of 90-95%, and use of a high ratio (80%) of mulberry tree sticks in the medium were critical to successful culture of Sanghuang.
31394179	10	11	with	brown	1755:1759	arg1	shape					1790:1794	a smaller and irregular shape	1766:1794	a smaller and irregular shape	1766:1794	The cultured mushrooms were yellow with a uniform shape, while the wild Sanghuang was dark brown with a smaller and irregular shape.
31394179	4	12	theme	quality	904:910	arg1	comparison					890:899	deep comparison	885:899	deep comparison of quality and pharmacological activities between the cultured and the wild Sanghuang	885:985	AIM OF THE STUDY This study aims to establish a practical protocol for culture, particularly for a bunch of production of Sanghuang mushrooms possibly to commercialize the cultured Sanghuang based on deep comparison of quality and pharmacological activities between the cultured and the wild Sanghuang.
31394179	12	13	theme	immunodeficient	2063:2077	arg1	mice					2079:2082	the immunodeficient mice	2059:2082	the immunodeficient mice	2059:2082	Use of a dose of 8 mg/kg or 16 mg/kg to immunoregenerate the immunodeficient mice was comparable between the cultured and wild Sanghang based on analysis of hematological parameters and histological examination of the thymus and spleen in the treated mice.
31394179	5	14	theme	wild	1061:1064	arg1	Sanghuang					1066:1074	the wild Sanghuang	1057:1074	the wild Sanghuang	1057:1074	MATERIALS AND METHODS A phylogenetic tree containing five strains of the wild Sanghuang was constructed using rDNA markers.
31394179	10	15	theme	wild	1731:1734	arg1	Sanghuang					1736:1744	the wild Sanghuang	1727:1744	the wild Sanghuang	1727:1744	The cultured mushrooms were yellow with a uniform shape, while the wild Sanghuang was dark brown with a smaller and irregular shape.
31394179	10	15	theme	wild	1731:1734	arg1	brown					1755:1759	brown	1755:1759	brown	1755:1759	The cultured mushrooms were yellow with a uniform shape, while the wild Sanghuang was dark brown with a smaller and irregular shape.
31394179	9	16	theme	22-28 °C	1497:1504	arg1	temperature					1482:1492	a temperature	1480:1492	a temperature of 22-28 °C and a high relative humidity of 90-95%	1480:1543	RESULTS Maintenance of a temperature of 22-28 °C and a high relative humidity of 90-95%, and use of a high ratio (80%) of mulberry tree sticks in the medium were critical to successful culture of Sanghuang.
31394179	9	17	theme	relative	1517:1524	arg1	humidity					1526:1533	a high relative humidity	1510:1533	a high relative humidity of 90-95%	1510:1543	RESULTS Maintenance of a temperature of 22-28 °C and a high relative humidity of 90-95%, and use of a high ratio (80%) of mulberry tree sticks in the medium were critical to successful culture of Sanghuang.
31394179	4	18	theme	pharmacological	916:930	arg1	activities					932:941	pharmacological activities	916:941	pharmacological activities	916:941	AIM OF THE STUDY This study aims to establish a practical protocol for culture, particularly for a bunch of production of Sanghuang mushrooms possibly to commercialize the cultured Sanghuang based on deep comparison of quality and pharmacological activities between the cultured and the wild Sanghuang.
31394179	12	19	from	spleen	2231:2236	arg1	mice					2253:2256	the treated mice	2241:2256	the treated mice	2241:2256	Use of a dose of 8 mg/kg or 16 mg/kg to immunoregenerate the immunodeficient mice was comparable between the cultured and wild Sanghang based on analysis of hematological parameters and histological examination of the thymus and spleen in the treated mice.
31394179	6	20	theme	mushrooms	1234:1242	arg1	culture					1209:1215	culture	1209:1215	culture of the Sanghuang mushrooms	1209:1242	Different temperatures and medium compositions were surveyed to develop a practical protocol for culture of the Sanghuang mushrooms.
31394179	4	21	theme	STUDY	696:700	arg1	AIM					685:687	AIM	685:687	AIM OF THE STUDY This study aims to establish a practical protocol for culture, particularly for a bunch of production of Sanghuang mushrooms	685:825	AIM OF THE STUDY This study aims to establish a practical protocol for culture, particularly for a bunch of production of Sanghuang mushrooms possibly to commercialize the cultured Sanghuang based on deep comparison of quality and pharmacological activities between the cultured and the wild Sanghuang.
31394179	13	22	theme	immunoregenerative	2314:2331	arg1	functions					2333:2341	the immunoregenerative functions	2310:2341	the immunoregenerative functions of the cultured Sanghuang	2310:2367	CONCLUSIONS This study highlights the potential of the immunoregenerative functions of the cultured Sanghuang for cancer chemotherapy and suggests that the cultured Sanghuang can be an alternative to wild Sanghuang used for nutraceutical medicine.
31394179	1	23	theme	ETHNOPHARMACOLOGICAL	124:143	arg1	Description					155:165	ETHNOPHARMACOLOGICAL RELEVANCE Description	124:165	ETHNOPHARMACOLOGICAL RELEVANCE Description of the pharmacological activities of Sanghuang mushrooms (Inonotus Sanghuang)	124:243	ETHNOPHARMACOLOGICAL RELEVANCE Description of the pharmacological activities of Sanghuang mushrooms (Inonotus Sanghuang) can be traced back to Tang dynasty of China 1300 years ago.
31394179	9	24	dep	RESULTS	1457:1463	arg1	Maintenance					1465:1475	Maintenance	1465:1475	Maintenance of a temperature of 22-28 °C and a high relative humidity of 90-95%	1465:1543	RESULTS Maintenance of a temperature of 22-28 °C and a high relative humidity of 90-95%, and use of a high ratio (80%) of mulberry tree sticks in the medium were critical to successful culture of Sanghuang.
31394179	8	25	theme	pharmacological	1337:1351	arg1	activities					1353:1362	pharmacological activities	1337:1362	pharmacological activities	1337:1362	Chemotherapeutic components and pharmacological activities were deeply analyzed between a cultured strain (SG) and three strains of the wild Sanghuang.
31394179	4	26	dep	aims	713:716	arg1	establish					721:729	establish	721:729	establish	721:729	AIM OF THE STUDY This study aims to establish a practical protocol for culture, particularly for a bunch of production of Sanghuang mushrooms possibly to commercialize the cultured Sanghuang based on deep comparison of quality and pharmacological activities between the cultured and the wild Sanghuang.
31394179	9	27	from	ratio	1564:1568	arg1	medium					1607:1612	the medium	1603:1612	the medium	1603:1612	RESULTS Maintenance of a temperature of 22-28 °C and a high relative humidity of 90-95%, and use of a high ratio (80%) of mulberry tree sticks in the medium were critical to successful culture of Sanghuang.
31394179	1	28	theme	RELEVANCE	145:153	arg1	Description					155:165	ETHNOPHARMACOLOGICAL RELEVANCE Description	124:165	ETHNOPHARMACOLOGICAL RELEVANCE Description of the pharmacological activities of Sanghuang mushrooms (Inonotus Sanghuang)	124:243	ETHNOPHARMACOLOGICAL RELEVANCE Description of the pharmacological activities of Sanghuang mushrooms (Inonotus Sanghuang) can be traced back to Tang dynasty of China 1300 years ago.
31394179	11	29	theme	water-soluble	1895:1907	arg1	nutraceuticals					1909:1922	water-soluble nutraceuticals	1895:1922	water-soluble nutraceuticals	1895:1922	The cultured mushrooms contained significantly higher levels of polysaccharides, amino acids, and water-soluble nutraceuticals, whereas flavones in the wild Sanghuang were significantly higher (P < 0.05).
31394179	12	30	theme	dose	2011:2014	arg1	Use					2002:2004	Use	2002:2004	Use of a dose of 8 mg/kg or 16 mg/kg to immunoregenerate the immunodeficient mice	2002:2082	Use of a dose of 8 mg/kg or 16 mg/kg to immunoregenerate the immunodeficient mice was comparable between the cultured and wild Sanghang based on analysis of hematological parameters and histological examination of the thymus and spleen in the treated mice.
31394179	11	31	theme	wild	1949:1952	arg1	Sanghuang					1954:1962	the wild Sanghuang	1945:1962	the wild Sanghuang	1945:1962	The cultured mushrooms contained significantly higher levels of polysaccharides, amino acids, and water-soluble nutraceuticals, whereas flavones in the wild Sanghuang were significantly higher (P < 0.05).
31394179	9	32	theme	%	1543:1543	arg1	humidity					1526:1533	a high relative humidity	1510:1533	a high relative humidity of 90-95%	1510:1543	RESULTS Maintenance of a temperature of 22-28 °C and a high relative humidity of 90-95%, and use of a high ratio (80%) of mulberry tree sticks in the medium were critical to successful culture of Sanghuang.
31394179	9	32	theme	%	1543:1543	arg1	22-28 °C					1497:1504	22-28 °C	1497:1504	22-28 °C	1497:1504	RESULTS Maintenance of a temperature of 22-28 °C and a high relative humidity of 90-95%, and use of a high ratio (80%) of mulberry tree sticks in the medium were critical to successful culture of Sanghuang.
31394179	12	33	theme	8 mg/kg	2019:2025	arg1	dose					2011:2014	a dose	2009:2014	a dose of 8 mg/kg or 16 mg/kg to immunoregenerate the immunodeficient mice	2009:2082	Use of a dose of 8 mg/kg or 16 mg/kg to immunoregenerate the immunodeficient mice was comparable between the cultured and wild Sanghang based on analysis of hematological parameters and histological examination of the thymus and spleen in the treated mice.
31394179	12	34	from	mice	2253:2256	arg1	analysis					2147:2154	analysis	2147:2154	analysis of hematological parameters	2147:2182	Use of a dose of 8 mg/kg or 16 mg/kg to immunoregenerate the immunodeficient mice was comparable between the cultured and wild Sanghang based on analysis of hematological parameters and histological examination of the thymus and spleen in the treated mice.
31394179	12	34	from	mice	2253:2256	arg1	examination					2201:2211	histological examination	2188:2211	histological examination of the thymus and spleen in the treated mice	2188:2256	Use of a dose of 8 mg/kg or 16 mg/kg to immunoregenerate the immunodeficient mice was comparable between the cultured and wild Sanghang based on analysis of hematological parameters and histological examination of the thymus and spleen in the treated mice.
31394179	1	35	theme	activities	190:199	arg1	Description					155:165	ETHNOPHARMACOLOGICAL RELEVANCE Description	124:165	ETHNOPHARMACOLOGICAL RELEVANCE Description of the pharmacological activities of Sanghuang mushrooms (Inonotus Sanghuang)	124:243	ETHNOPHARMACOLOGICAL RELEVANCE Description of the pharmacological activities of Sanghuang mushrooms (Inonotus Sanghuang) can be traced back to Tang dynasty of China 1300 years ago.
31394179	9	36	from	use	1550:1552	arg1	medium					1607:1612	the medium	1603:1612	the medium	1603:1612	RESULTS Maintenance of a temperature of 22-28 °C and a high relative humidity of 90-95%, and use of a high ratio (80%) of mulberry tree sticks in the medium were critical to successful culture of Sanghuang.
31394179	13	37	dep	CONCLUSIONS	2259:2269	arg1	suggests					2397:2404	suggests	2397:2404	suggests that the cultured Sanghuang can be an alternative to wild Sanghuang used for nutraceutical medicine	2397:2504	CONCLUSIONS This study highlights the potential of the immunoregenerative functions of the cultured Sanghuang for cancer chemotherapy and suggests that the cultured Sanghuang can be an alternative to wild Sanghuang used for nutraceutical medicine.
31394179	13	37	dep	CONCLUSIONS	2259:2269	arg1	highlights					2282:2291	highlights	2282:2291	highlights the potential of the immunoregenerative functions of the cultured Sanghuang for cancer chemotherapy	2282:2391	CONCLUSIONS This study highlights the potential of the immunoregenerative functions of the cultured Sanghuang for cancer chemotherapy and suggests that the cultured Sanghuang can be an alternative to wild Sanghuang used for nutraceutical medicine.
31394179	4	38	dep	AIM	685:687	arg1	aims					713:716	aims	713:716	aims to establish a practical protocol for culture, particularly for a bunch of production of Sanghuang mushrooms	713:825	AIM OF THE STUDY This study aims to establish a practical protocol for culture, particularly for a bunch of production of Sanghuang mushrooms possibly to commercialize the cultured Sanghuang based on deep comparison of quality and pharmacological activities between the cultured and the wild Sanghuang.
31394179	2	39	theme	tumorigenesis	507:519	arg1	inhibition					493:502	inhibition	493:502	inhibition of tumorigenesis	493:519	This mushroom has been widely accepted in China, Japan, Korea and certain regions of Europe as a nutraceutical medicine for enhancing immunity or an alternative medicine for prevention or inhibition of tumorigenesis.
31394179	2	39	theme	tumorigenesis	507:519	arg1	prevention					479:488	prevention	479:488	prevention	479:488	This mushroom has been widely accepted in China, Japan, Korea and certain regions of Europe as a nutraceutical medicine for enhancing immunity or an alternative medicine for prevention or inhibition of tumorigenesis.
31394179	12	40	theme	spleen	2231:2236	arg1	analysis					2147:2154	analysis	2147:2154	analysis of hematological parameters	2147:2182	Use of a dose of 8 mg/kg or 16 mg/kg to immunoregenerate the immunodeficient mice was comparable between the cultured and wild Sanghang based on analysis of hematological parameters and histological examination of the thymus and spleen in the treated mice.
31394179	12	40	theme	spleen	2231:2236	arg1	examination					2201:2211	histological examination	2188:2211	histological examination of the thymus and spleen in the treated mice	2188:2256	Use of a dose of 8 mg/kg or 16 mg/kg to immunoregenerate the immunodeficient mice was comparable between the cultured and wild Sanghang based on analysis of hematological parameters and histological examination of the thymus and spleen in the treated mice.
31394179	11	41	theme	amino	1878:1882	arg1	acids					1884:1888	amino acids	1878:1888	amino acids	1878:1888	The cultured mushrooms contained significantly higher levels of polysaccharides, amino acids, and water-soluble nutraceuticals, whereas flavones in the wild Sanghuang were significantly higher (P < 0.05).
31394179	0	42	theme	immunodeficient	102:116	arg1	mice					118:121	the immunodeficient mice	98:121	the immunodeficient mice	98:121	Immunoregenerative effects of the bionically cultured Sanghuang mushrooms (Inonotus sanghuagn) on the immunodeficient mice.
31394179	9	43	theme	high	1559:1562	arg1	ratio					1564:1568	a high ratio	1557:1568	a high ratio (80%) of mulberry tree sticks in the medium	1557:1612	RESULTS Maintenance of a temperature of 22-28 °C and a high relative humidity of 90-95%, and use of a high ratio (80%) of mulberry tree sticks in the medium were critical to successful culture of Sanghuang.
31394179	9	43	theme	high	1559:1562	arg1	%					1573:1573	80%	1571:1573	80%	1571:1573	RESULTS Maintenance of a temperature of 22-28 °C and a high relative humidity of 90-95%, and use of a high ratio (80%) of mulberry tree sticks in the medium were critical to successful culture of Sanghuang.
31394179	12	44	theme	thymus	2220:2225	arg1	analysis					2147:2154	analysis	2147:2154	analysis of hematological parameters	2147:2182	Use of a dose of 8 mg/kg or 16 mg/kg to immunoregenerate the immunodeficient mice was comparable between the cultured and wild Sanghang based on analysis of hematological parameters and histological examination of the thymus and spleen in the treated mice.
31394179	12	44	theme	thymus	2220:2225	arg1	examination					2201:2211	histological examination	2188:2211	histological examination of the thymus and spleen in the treated mice	2188:2256	Use of a dose of 8 mg/kg or 16 mg/kg to immunoregenerate the immunodeficient mice was comparable between the cultured and wild Sanghang based on analysis of hematological parameters and histological examination of the thymus and spleen in the treated mice.
31394179	2	45	theme	certain	371:377	arg1	regions					379:385	certain regions	371:385	certain regions of Europe	371:395	This mushroom has been widely accepted in China, Japan, Korea and certain regions of Europe as a nutraceutical medicine for enhancing immunity or an alternative medicine for prevention or inhibition of tumorigenesis.
31394179	0	46	theme	Immunoregenerative	0:17	arg1	effects					19:25	Immunoregenerative effects	0:25	Immunoregenerative effects of the bionically cultured Sanghuang mushrooms (Inonotus sanghuagn) on the immunodeficient mice.	0:122	Immunoregenerative effects of the bionically cultured Sanghuang mushrooms (Inonotus sanghuagn) on the immunodeficient mice.
31394179	3	47	from	trees	583:587	arg1	available					555:563	available	555:563	available	555:563	However, this mushroom is rarely available from the mulberry trees in the wild because of the rigorous conditions needed for formation of the Sanghuang mushrooms.
31394179	3	47	from	trees	583:587	arg1	wild					596:599	wild	596:599	wild	596:599	However, this mushroom is rarely available from the mulberry trees in the wild because of the rigorous conditions needed for formation of the Sanghuang mushrooms.
31394179	2	48	theme	nutraceutical	402:414	arg1	mushroom					310:317	This mushroom	305:317	This mushroom	305:317	This mushroom has been widely accepted in China, Japan, Korea and certain regions of Europe as a nutraceutical medicine for enhancing immunity or an alternative medicine for prevention or inhibition of tumorigenesis.
31394179	2	48	theme	nutraceutical	402:414	arg1	medicine					416:423	a nutraceutical medicine	400:423	a nutraceutical medicine for enhancing immunity	400:446	This mushroom has been widely accepted in China, Japan, Korea and certain regions of Europe as a nutraceutical medicine for enhancing immunity or an alternative medicine for prevention or inhibition of tumorigenesis.
31394179	9	49	from	RESULTS	1457:1463	arg1	medium					1607:1612	the medium	1603:1612	the medium	1603:1612	RESULTS Maintenance of a temperature of 22-28 °C and a high relative humidity of 90-95%, and use of a high ratio (80%) of mulberry tree sticks in the medium were critical to successful culture of Sanghuang.
31394179	13	50	theme	cultured	2415:2422	arg1	alternative					2444:2454	an alternative	2441:2454	an alternative to wild Sanghuang used for nutraceutical medicine	2441:2504	CONCLUSIONS This study highlights the potential of the immunoregenerative functions of the cultured Sanghuang for cancer chemotherapy and suggests that the cultured Sanghuang can be an alternative to wild Sanghuang used for nutraceutical medicine.
31394179	13	50	theme	cultured	2415:2422	arg1	Sanghuang					2424:2432	the cultured Sanghuang	2411:2432	the cultured Sanghuang	2411:2432	CONCLUSIONS This study highlights the potential of the immunoregenerative functions of the cultured Sanghuang for cancer chemotherapy and suggests that the cultured Sanghuang can be an alternative to wild Sanghuang used for nutraceutical medicine.
31394179	5	51	theme	phylogenetic	1012:1023	arg1	tree					1025:1028	A phylogenetic tree	1010:1028	A phylogenetic tree	1010:1028	MATERIALS AND METHODS A phylogenetic tree containing five strains of the wild Sanghuang was constructed using rDNA markers.
31394179	12	52	theme	hematological	2159:2171	arg1	parameters					2173:2182	hematological parameters	2159:2182	hematological parameters	2159:2182	Use of a dose of 8 mg/kg or 16 mg/kg to immunoregenerate the immunodeficient mice was comparable between the cultured and wild Sanghang based on analysis of hematological parameters and histological examination of the thymus and spleen in the treated mice.
31394179	0	53	theme	Sanghuang	54:62	arg1	sanghuagn					84:92	sanghuagn	84:92	sanghuagn	84:92	Immunoregenerative effects of the bionically cultured Sanghuang mushrooms (Inonotus sanghuagn) on the immunodeficient mice.
31394179	0	53	theme	Sanghuang	54:62	arg1	mushrooms					64:72	the bionically cultured Sanghuang mushrooms	30:72	the bionically cultured Sanghuang mushrooms (Inonotus sanghuagn)	30:93	Immunoregenerative effects of the bionically cultured Sanghuang mushrooms (Inonotus sanghuagn) on the immunodeficient mice.
31394179	9	54	theme	tree	1588:1591	arg1	sticks					1593:1598	mulberry tree sticks	1579:1598	mulberry tree sticks	1579:1598	RESULTS Maintenance of a temperature of 22-28 °C and a high relative humidity of 90-95%, and use of a high ratio (80%) of mulberry tree sticks in the medium were critical to successful culture of Sanghuang.
31394179	9	55	theme	successful	1631:1640	arg1	culture					1642:1648	successful culture	1631:1648	successful culture of Sanghuang	1631:1661	RESULTS Maintenance of a temperature of 22-28 °C and a high relative humidity of 90-95%, and use of a high ratio (80%) of mulberry tree sticks in the medium were critical to successful culture of Sanghuang.
31394179	5	56	contain	containing	1030:1039	arg1	MATERIALS					988:996	MATERIALS	988:996	MATERIALS	988:996	MATERIALS AND METHODS A phylogenetic tree containing five strains of the wild Sanghuang was constructed using rDNA markers.
31394179	5	56	contain	containing	1030:1039	arg2	strains					1046:1052	five strains	1041:1052	five strains of the wild Sanghuang	1041:1074	MATERIALS AND METHODS A phylogenetic tree containing five strains of the wild Sanghuang was constructed using rDNA markers.
31394179	3	57	theme	Sanghuang	664:672	arg1	mushrooms					674:682	the Sanghuang mushrooms	660:682	the Sanghuang mushrooms	660:682	However, this mushroom is rarely available from the mulberry trees in the wild because of the rigorous conditions needed for formation of the Sanghuang mushrooms.
31394179	8	58	theme	Sanghuang	1446:1454	arg1	SG					1412:1413	SG	1412:1413	SG	1412:1413	Chemotherapeutic components and pharmacological activities were deeply analyzed between a cultured strain (SG) and three strains of the wild Sanghuang.
31394179	8	58	theme	Sanghuang	1446:1454	arg1	strain					1404:1409	a cultured strain	1393:1409	a cultured strain (SG)	1393:1414	Chemotherapeutic components and pharmacological activities were deeply analyzed between a cultured strain (SG) and three strains of the wild Sanghuang.
31394179	8	58	theme	Sanghuang	1446:1454	arg1	strains					1426:1432	three strains	1420:1432	three strains of the wild Sanghuang	1420:1454	Chemotherapeutic components and pharmacological activities were deeply analyzed between a cultured strain (SG) and three strains of the wild Sanghuang.
31394179	13	59	theme	cancer	2373:2378	arg1	chemotherapy					2380:2391	cancer chemotherapy	2373:2391	cancer chemotherapy	2373:2391	CONCLUSIONS This study highlights the potential of the immunoregenerative functions of the cultured Sanghuang for cancer chemotherapy and suggests that the cultured Sanghuang can be an alternative to wild Sanghuang used for nutraceutical medicine.
31394179	10	60	theme	cultured	1668:1675	arg1	mushrooms					1677:1685	The cultured mushrooms	1664:1685	The cultured mushrooms	1664:1685	The cultured mushrooms were yellow with a uniform shape, while the wild Sanghuang was dark brown with a smaller and irregular shape.
31394179	10	60	theme	cultured	1668:1675	arg1	yellow					1692:1697	yellow	1692:1697	yellow	1692:1697	The cultured mushrooms were yellow with a uniform shape, while the wild Sanghuang was dark brown with a smaller and irregular shape.
31394179	7	61	theme	immunodeficient	1283:1297	arg1	mice					1299:1302	the immunodeficient mice	1279:1302	the immunodeficient mice	1279:1302	5-fluorouracil was used to induce the immunodeficient mice.
31394179	10	62	theme	uniform	1706:1712	arg1	shape					1714:1718	a uniform shape	1704:1718	a uniform shape	1704:1718	The cultured mushrooms were yellow with a uniform shape, while the wild Sanghuang was dark brown with a smaller and irregular shape.
31394179	13	63	theme	Sanghuang	2359:2367	arg1	functions					2333:2341	the immunoregenerative functions	2310:2341	the immunoregenerative functions of the cultured Sanghuang	2310:2367	CONCLUSIONS This study highlights the potential of the immunoregenerative functions of the cultured Sanghuang for cancer chemotherapy and suggests that the cultured Sanghuang can be an alternative to wild Sanghuang used for nutraceutical medicine.
31394179	1	64	theme	Tang	267:270	arg1	dynasty					272:278	Tang dynasty	267:278	Tang dynasty of China 1300 years ago	267:302	ETHNOPHARMACOLOGICAL RELEVANCE Description of the pharmacological activities of Sanghuang mushrooms (Inonotus Sanghuang) can be traced back to Tang dynasty of China 1300 years ago.
31394179	4	65	theme	cultured	857:864	arg1	Sanghuang					866:874	the cultured Sanghuang	853:874	the cultured Sanghuang based on deep comparison of quality and pharmacological activities between the cultured and the wild Sanghuang	853:985	AIM OF THE STUDY This study aims to establish a practical protocol for culture, particularly for a bunch of production of Sanghuang mushrooms possibly to commercialize the cultured Sanghuang based on deep comparison of quality and pharmacological activities between the cultured and the wild Sanghuang.
31394179	6	66	theme	practical	1186:1194	arg1	protocol					1196:1203	a practical protocol	1184:1203	a practical protocol for culture of the Sanghuang mushrooms	1184:1242	Different temperatures and medium compositions were surveyed to develop a practical protocol for culture of the Sanghuang mushrooms.
31394179	12	67	from	thymus	2220:2225	arg1	mice					2253:2256	the treated mice	2241:2256	the treated mice	2241:2256	Use of a dose of 8 mg/kg or 16 mg/kg to immunoregenerate the immunodeficient mice was comparable between the cultured and wild Sanghang based on analysis of hematological parameters and histological examination of the thymus and spleen in the treated mice.
31394179	4	68	theme	Sanghuang	807:815	arg1	mushrooms					817:825	Sanghuang mushrooms	807:825	Sanghuang mushrooms	807:825	AIM OF THE STUDY This study aims to establish a practical protocol for culture, particularly for a bunch of production of Sanghuang mushrooms possibly to commercialize the cultured Sanghuang based on deep comparison of quality and pharmacological activities between the cultured and the wild Sanghuang.
31394179	1	69	theme	mushrooms	214:222	arg1	activities					190:199	the pharmacological activities	170:199	the pharmacological activities of Sanghuang mushrooms (Inonotus Sanghuang)	170:243	ETHNOPHARMACOLOGICAL RELEVANCE Description of the pharmacological activities of Sanghuang mushrooms (Inonotus Sanghuang) can be traced back to Tang dynasty of China 1300 years ago.
31394179	4	70	dep	cultured	955:962	arg1	the					951:953	the	951:953	the	951:953	AIM OF THE STUDY This study aims to establish a practical protocol for culture, particularly for a bunch of production of Sanghuang mushrooms possibly to commercialize the cultured Sanghuang based on deep comparison of quality and pharmacological activities between the cultured and the wild Sanghuang.
31394179	11	71	from	flavones	1933:1940	arg1	Sanghuang					1954:1962	the wild Sanghuang	1945:1962	the wild Sanghuang	1945:1962	The cultured mushrooms contained significantly higher levels of polysaccharides, amino acids, and water-soluble nutraceuticals, whereas flavones in the wild Sanghuang were significantly higher (P < 0.05).
31394179	13	72	theme	nutraceutical	2483:2495	arg1	medicine					2497:2504	nutraceutical medicine	2483:2504	nutraceutical medicine	2483:2504	CONCLUSIONS This study highlights the potential of the immunoregenerative functions of the cultured Sanghuang for cancer chemotherapy and suggests that the cultured Sanghuang can be an alternative to wild Sanghuang used for nutraceutical medicine.
31394179	5	73	theme	Sanghuang	1066:1074	arg1	strains					1046:1052	five strains	1041:1052	five strains of the wild Sanghuang	1041:1074	MATERIALS AND METHODS A phylogenetic tree containing five strains of the wild Sanghuang was constructed using rDNA markers.
31394179	10	74	theme	smaller	1768:1774	arg1	shape					1790:1794	a smaller and irregular shape	1766:1794	a smaller and irregular shape	1766:1794	The cultured mushrooms were yellow with a uniform shape, while the wild Sanghuang was dark brown with a smaller and irregular shape.
31394179	13	75	theme	functions	2333:2341	arg1	potential					2297:2305	the potential	2293:2305	the potential of the immunoregenerative functions of the cultured Sanghuang for cancer chemotherapy	2293:2391	CONCLUSIONS This study highlights the potential of the immunoregenerative functions of the cultured Sanghuang for cancer chemotherapy and suggests that the cultured Sanghuang can be an alternative to wild Sanghuang used for nutraceutical medicine.
31394179	5	76	dep	MATERIALS	988:996	arg1	tree					1025:1028	A phylogenetic tree	1010:1028	A phylogenetic tree	1010:1028	MATERIALS AND METHODS A phylogenetic tree containing five strains of the wild Sanghuang was constructed using rDNA markers.
31394179	3	77	theme	mulberry	574:581	arg1	trees					583:587	the mulberry trees	570:587	the mulberry trees in the wild because of the rigorous conditions needed for formation of the Sanghuang mushrooms	570:682	However, this mushroom is rarely available from the mulberry trees in the wild because of the rigorous conditions needed for formation of the Sanghuang mushrooms.
31394179	9	78	theme	high	1512:1515	arg1	humidity					1526:1533	a high relative humidity	1510:1533	a high relative humidity of 90-95%	1510:1543	RESULTS Maintenance of a temperature of 22-28 °C and a high relative humidity of 90-95%, and use of a high ratio (80%) of mulberry tree sticks in the medium were critical to successful culture of Sanghuang.
31394179	6	79	theme	Sanghuang	1224:1232	arg1	mushrooms					1234:1242	the Sanghuang mushrooms	1220:1242	the Sanghuang mushrooms	1220:1242	Different temperatures and medium compositions were surveyed to develop a practical protocol for culture of the Sanghuang mushrooms.
31394179	8	80	theme	Chemotherapeutic	1305:1320	arg1	components					1322:1331	Chemotherapeutic components	1305:1331	Chemotherapeutic components	1305:1331	Chemotherapeutic components and pharmacological activities were deeply analyzed between a cultured strain (SG) and three strains of the wild Sanghuang.
31394179	13	81	theme	wild	2459:2462	arg1	Sanghuang					2464:2472	wild Sanghuang	2459:2472	wild Sanghuang used for nutraceutical medicine	2459:2504	CONCLUSIONS This study highlights the potential of the immunoregenerative functions of the cultured Sanghuang for cancer chemotherapy and suggests that the cultured Sanghuang can be an alternative to wild Sanghuang used for nutraceutical medicine.
31394179	7	82	used	used	1264:1267	arg2	5-fluorouracil					1245:1258	5-fluorouracil	1245:1258	5-fluorouracil	1245:1258	5-fluorouracil was used to induce the immunodeficient mice.
31394179	9	83	theme	humidity	1526:1533	arg1	temperature					1482:1492	a temperature	1480:1492	a temperature of 22-28 °C and a high relative humidity of 90-95%	1480:1543	RESULTS Maintenance of a temperature of 22-28 °C and a high relative humidity of 90-95%, and use of a high ratio (80%) of mulberry tree sticks in the medium were critical to successful culture of Sanghuang.
31394179	4	84	theme	deep	885:888	arg1	comparison					890:899	deep comparison	885:899	deep comparison of quality and pharmacological activities between the cultured and the wild Sanghuang	885:985	AIM OF THE STUDY This study aims to establish a practical protocol for culture, particularly for a bunch of production of Sanghuang mushrooms possibly to commercialize the cultured Sanghuang based on deep comparison of quality and pharmacological activities between the cultured and the wild Sanghuang.
31394179	10	85	theme	irregular	1780:1788	arg1	shape					1790:1794	a smaller and irregular shape	1766:1794	a smaller and irregular shape	1766:1794	The cultured mushrooms were yellow with a uniform shape, while the wild Sanghuang was dark brown with a smaller and irregular shape.
31394179	1	86	theme	pharmacological	174:188	arg1	activities					190:199	the pharmacological activities	170:199	the pharmacological activities of Sanghuang mushrooms (Inonotus Sanghuang)	170:243	ETHNOPHARMACOLOGICAL RELEVANCE Description of the pharmacological activities of Sanghuang mushrooms (Inonotus Sanghuang) can be traced back to Tang dynasty of China 1300 years ago.
31394179	4	87	theme	wild	972:975	arg1	Sanghuang					977:985	the wild Sanghuang	968:985	the wild Sanghuang	968:985	AIM OF THE STUDY This study aims to establish a practical protocol for culture, particularly for a bunch of production of Sanghuang mushrooms possibly to commercialize the cultured Sanghuang based on deep comparison of quality and pharmacological activities between the cultured and the wild Sanghuang.
31394179	0	88	from	effects	19:25	arg1	mice					118:121	the immunodeficient mice	98:121	the immunodeficient mice	98:121	Immunoregenerative effects of the bionically cultured Sanghuang mushrooms (Inonotus sanghuagn) on the immunodeficient mice.
31394179	4	89	theme	activities	932:941	arg1	comparison					890:899	deep comparison	885:899	deep comparison of quality and pharmacological activities between the cultured and the wild Sanghuang	885:985	AIM OF THE STUDY This study aims to establish a practical protocol for culture, particularly for a bunch of production of Sanghuang mushrooms possibly to commercialize the cultured Sanghuang based on deep comparison of quality and pharmacological activities between the cultured and the wild Sanghuang.
31394179	4	90	theme	practical	733:741	arg1	protocol					743:750	a practical protocol	731:750	a practical protocol for culture	731:762	AIM OF THE STUDY This study aims to establish a practical protocol for culture, particularly for a bunch of production of Sanghuang mushrooms possibly to commercialize the cultured Sanghuang based on deep comparison of quality and pharmacological activities between the cultured and the wild Sanghuang.
31394179	6	91	theme	Different	1112:1120	arg1	temperatures					1122:1133	Different temperatures	1112:1133	Different temperatures	1112:1133	Different temperatures and medium compositions were surveyed to develop a practical protocol for culture of the Sanghuang mushrooms.
31394179	5	92	theme	rDNA	1098:1101	arg1	markers					1103:1109	rDNA markers	1098:1109	rDNA markers	1098:1109	MATERIALS AND METHODS A phylogenetic tree containing five strains of the wild Sanghuang was constructed using rDNA markers.
31394179	4	93	dep	production	793:802	arg1	a					782:782	a	782:782	a	782:782	AIM OF THE STUDY This study aims to establish a practical protocol for culture, particularly for a bunch of production of Sanghuang mushrooms possibly to commercialize the cultured Sanghuang based on deep comparison of quality and pharmacological activities between the cultured and the wild Sanghuang.
31394179	4	93	dep	production	793:802	arg1	bunch					784:788	bunch	784:788	bunch	784:788	AIM OF THE STUDY This study aims to establish a practical protocol for culture, particularly for a bunch of production of Sanghuang mushrooms possibly to commercialize the cultured Sanghuang based on deep comparison of quality and pharmacological activities between the cultured and the wild Sanghuang.
31394179	12	94	dep	dose	2011:2014	arg1	immunoregenerate					2042:2057	immunoregenerate	2042:2057	to immunoregenerate the immunodeficient mice	2039:2082	Use of a dose of 8 mg/kg or 16 mg/kg to immunoregenerate the immunodeficient mice was comparable between the cultured and wild Sanghang based on analysis of hematological parameters and histological examination of the thymus and spleen in the treated mice.
31394179	9	95	from	medium	1607:1612	arg1	use					1550:1552	use	1550:1552	use of a high ratio (80%) of mulberry tree sticks in the medium	1550:1612	RESULTS Maintenance of a temperature of 22-28 °C and a high relative humidity of 90-95%, and use of a high ratio (80%) of mulberry tree sticks in the medium were critical to successful culture of Sanghuang.
31394179	9	95	from	medium	1607:1612	arg1	RESULTS					1457:1463	RESULTS	1457:1463	RESULTS	1457:1463	RESULTS Maintenance of a temperature of 22-28 °C and a high relative humidity of 90-95%, and use of a high ratio (80%) of mulberry tree sticks in the medium were critical to successful culture of Sanghuang.
31394179	12	96	theme	treated	2245:2251	arg1	mice					2253:2256	the treated mice	2241:2256	the treated mice	2241:2256	Use of a dose of 8 mg/kg or 16 mg/kg to immunoregenerate the immunodeficient mice was comparable between the cultured and wild Sanghang based on analysis of hematological parameters and histological examination of the thymus and spleen in the treated mice.
31394179	8	97	theme	cultured	1395:1402	arg1	SG					1412:1413	SG	1412:1413	SG	1412:1413	Chemotherapeutic components and pharmacological activities were deeply analyzed between a cultured strain (SG) and three strains of the wild Sanghuang.
31394179	8	97	theme	cultured	1395:1402	arg1	strain					1404:1409	a cultured strain	1393:1409	a cultured strain (SG)	1393:1414	Chemotherapeutic components and pharmacological activities were deeply analyzed between a cultured strain (SG) and three strains of the wild Sanghuang.
31394179	11	98	theme	acids	1884:1888	arg1	levels					1851:1856	significantly higher levels	1830:1856	significantly higher levels of polysaccharides, amino acids, and water-soluble nutraceuticals	1830:1922	The cultured mushrooms contained significantly higher levels of polysaccharides, amino acids, and water-soluble nutraceuticals, whereas flavones in the wild Sanghuang were significantly higher (P < 0.05).
31394179	9	99	theme	ratio	1564:1568	arg1	use					1550:1552	use	1550:1552	use of a high ratio (80%) of mulberry tree sticks in the medium	1550:1612	RESULTS Maintenance of a temperature of 22-28 °C and a high relative humidity of 90-95%, and use of a high ratio (80%) of mulberry tree sticks in the medium were critical to successful culture of Sanghuang.
31394179	9	99	theme	ratio	1564:1568	arg1	RESULTS					1457:1463	RESULTS	1457:1463	RESULTS	1457:1463	RESULTS Maintenance of a temperature of 22-28 °C and a high relative humidity of 90-95%, and use of a high ratio (80%) of mulberry tree sticks in the medium were critical to successful culture of Sanghuang.
31394179	2	100	theme	enhancing	429:437	arg1	immunity					439:446	enhancing immunity	429:446	enhancing immunity	429:446	This mushroom has been widely accepted in China, Japan, Korea and certain regions of Europe as a nutraceutical medicine for enhancing immunity or an alternative medicine for prevention or inhibition of tumorigenesis.
31394179	12	101	theme	parameters	2173:2182	arg1	analysis					2147:2154	analysis	2147:2154	analysis of hematological parameters	2147:2182	Use of a dose of 8 mg/kg or 16 mg/kg to immunoregenerate the immunodeficient mice was comparable between the cultured and wild Sanghang based on analysis of hematological parameters and histological examination of the thymus and spleen in the treated mice.
31394179	12	101	theme	parameters	2173:2182	arg1	examination					2201:2211	histological examination	2188:2211	histological examination of the thymus and spleen in the treated mice	2188:2256	Use of a dose of 8 mg/kg or 16 mg/kg to immunoregenerate the immunodeficient mice was comparable between the cultured and wild Sanghang based on analysis of hematological parameters and histological examination of the thymus and spleen in the treated mice.
31394179	12	102	theme	histological	2188:2199	arg1	examination					2201:2211	histological examination	2188:2211	histological examination of the thymus and spleen in the treated mice	2188:2256	Use of a dose of 8 mg/kg or 16 mg/kg to immunoregenerate the immunodeficient mice was comparable between the cultured and wild Sanghang based on analysis of hematological parameters and histological examination of the thymus and spleen in the treated mice.
31394179	12	103	theme	wild	2124:2127	arg1	Sanghang					2129:2136	wild Sanghang	2124:2136	wild Sanghang	2124:2136	Use of a dose of 8 mg/kg or 16 mg/kg to immunoregenerate the immunodeficient mice was comparable between the cultured and wild Sanghang based on analysis of hematological parameters and histological examination of the thymus and spleen in the treated mice.
31394179	11	104	theme	nutraceuticals	1909:1922	arg1	levels					1851:1856	significantly higher levels	1830:1856	significantly higher levels of polysaccharides, amino acids, and water-soluble nutraceuticals	1830:1922	The cultured mushrooms contained significantly higher levels of polysaccharides, amino acids, and water-soluble nutraceuticals, whereas flavones in the wild Sanghuang were significantly higher (P < 0.05).
31394179	0	105	theme	cultured	45:52	arg1	sanghuagn					84:92	sanghuagn	84:92	sanghuagn	84:92	Immunoregenerative effects of the bionically cultured Sanghuang mushrooms (Inonotus sanghuagn) on the immunodeficient mice.
31394179	0	105	theme	cultured	45:52	arg1	mushrooms					64:72	the bionically cultured Sanghuang mushrooms	30:72	the bionically cultured Sanghuang mushrooms (Inonotus sanghuagn)	30:93	Immunoregenerative effects of the bionically cultured Sanghuang mushrooms (Inonotus sanghuagn) on the immunodeficient mice.
31394179	2	106	theme	alternative	454:464	arg1	mushroom					310:317	This mushroom	305:317	This mushroom	305:317	This mushroom has been widely accepted in China, Japan, Korea and certain regions of Europe as a nutraceutical medicine for enhancing immunity or an alternative medicine for prevention or inhibition of tumorigenesis.
31394179	2	106	theme	alternative	454:464	arg1	medicine					466:473	an alternative medicine	451:473	an alternative medicine for prevention or inhibition of tumorigenesis	451:519	This mushroom has been widely accepted in China, Japan, Korea and certain regions of Europe as a nutraceutical medicine for enhancing immunity or an alternative medicine for prevention or inhibition of tumorigenesis.
31394179	3	107	theme	rigorous	616:623	arg1	conditions					625:634	the rigorous conditions	612:634	the rigorous conditions needed for formation of the Sanghuang mushrooms	612:682	However, this mushroom is rarely available from the mulberry trees in the wild because of the rigorous conditions needed for formation of the Sanghuang mushrooms.
31394179	2	108	theme	Europe	390:395	arg1	regions					379:385	certain regions	371:385	certain regions of Europe	371:395	This mushroom has been widely accepted in China, Japan, Korea and certain regions of Europe as a nutraceutical medicine for enhancing immunity or an alternative medicine for prevention or inhibition of tumorigenesis.
31394179	0	109	theme	mushrooms	64:72	arg1	effects					19:25	Immunoregenerative effects	0:25	Immunoregenerative effects of the bionically cultured Sanghuang mushrooms (Inonotus sanghuagn) on the immunodeficient mice.	0:122	Immunoregenerative effects of the bionically cultured Sanghuang mushrooms (Inonotus sanghuagn) on the immunodeficient mice.
31394179	9	110	theme	mulberry	1579:1586	arg1	sticks					1593:1598	mulberry tree sticks	1579:1598	mulberry tree sticks	1579:1598	RESULTS Maintenance of a temperature of 22-28 °C and a high relative humidity of 90-95%, and use of a high ratio (80%) of mulberry tree sticks in the medium were critical to successful culture of Sanghuang.
31394179	6	111	theme	medium	1139:1144	arg1	compositions					1146:1157	medium compositions	1139:1157	medium compositions	1139:1157	Different temperatures and medium compositions were surveyed to develop a practical protocol for culture of the Sanghuang mushrooms.
31394179	11	112	contain	contained	1820:1828	arg1	mushrooms					1810:1818	The cultured mushrooms	1797:1818	The cultured mushrooms	1797:1818	The cultured mushrooms contained significantly higher levels of polysaccharides, amino acids, and water-soluble nutraceuticals, whereas flavones in the wild Sanghuang were significantly higher (P < 0.05).
31394179	11	112	contain	contained	1820:1828	arg2	levels					1851:1856	significantly higher levels	1830:1856	significantly higher levels of polysaccharides, amino acids, and water-soluble nutraceuticals	1830:1922	The cultured mushrooms contained significantly higher levels of polysaccharides, amino acids, and water-soluble nutraceuticals, whereas flavones in the wild Sanghuang were significantly higher (P < 0.05).
31394179	10	113	with	yellow	1692:1697	arg1	shape					1714:1718	a uniform shape	1704:1718	a uniform shape	1704:1718	The cultured mushrooms were yellow with a uniform shape, while the wild Sanghuang was dark brown with a smaller and irregular shape.
31394179	9	114	theme	sticks	1593:1598	arg1	ratio					1564:1568	a high ratio	1557:1568	a high ratio (80%) of mulberry tree sticks in the medium	1557:1612	RESULTS Maintenance of a temperature of 22-28 °C and a high relative humidity of 90-95%, and use of a high ratio (80%) of mulberry tree sticks in the medium were critical to successful culture of Sanghuang.
31394179	9	114	theme	sticks	1593:1598	arg1	%					1573:1573	80%	1571:1573	80%	1571:1573	RESULTS Maintenance of a temperature of 22-28 °C and a high relative humidity of 90-95%, and use of a high ratio (80%) of mulberry tree sticks in the medium were critical to successful culture of Sanghuang.
31394179	11	115	dep	contained	1820:1828	arg1	whereas					1925:1931	whereas	1925:1931	whereas	1925:1931	The cultured mushrooms contained significantly higher levels of polysaccharides, amino acids, and water-soluble nutraceuticals, whereas flavones in the wild Sanghuang were significantly higher (P < 0.05).
31394179	8	116	theme	wild	1441:1444	arg1	Sanghuang					1446:1454	the wild Sanghuang	1437:1454	the wild Sanghuang	1437:1454	Chemotherapeutic components and pharmacological activities were deeply analyzed between a cultured strain (SG) and three strains of the wild Sanghuang.
31394179	11	117	theme	cultured	1801:1808	arg1	mushrooms					1810:1818	The cultured mushrooms	1797:1818	The cultured mushrooms	1797:1818	The cultured mushrooms contained significantly higher levels of polysaccharides, amino acids, and water-soluble nutraceuticals, whereas flavones in the wild Sanghuang were significantly higher (P < 0.05).
31394179	12	118	from	analysis	2147:2154	arg1	mice					2253:2256	the treated mice	2241:2256	the treated mice	2241:2256	Use of a dose of 8 mg/kg or 16 mg/kg to immunoregenerate the immunodeficient mice was comparable between the cultured and wild Sanghang based on analysis of hematological parameters and histological examination of the thymus and spleen in the treated mice.
31394179	13	119	theme	cultured	2350:2357	arg1	Sanghuang					2359:2367	the cultured Sanghuang	2346:2367	the cultured Sanghuang	2346:2367	CONCLUSIONS This study highlights the potential of the immunoregenerative functions of the cultured Sanghuang for cancer chemotherapy and suggests that the cultured Sanghuang can be an alternative to wild Sanghuang used for nutraceutical medicine.
30102748	2	0	theme	membrane-anchored	456:472	arg1	SMc02432					511:518	SMc02432	511:518	SMc02432	511:518	Here we show that the seven-transmembrane receptor (7TMR) protein RgsP (SMc00074), together with the putative membrane-anchored peptidoglycan metallopeptidase RgsM (SMc02432), have key roles in unipolar peptidoglycan formation during growth and at mid-cell during cell division in Sinorhizobium meliloti.
30102748	2	0	theme	membrane-anchored	456:472	arg1	RgsM					505:508	the putative membrane-anchored peptidoglycan metallopeptidase RgsM	443:508	the putative membrane-anchored peptidoglycan metallopeptidase RgsM (SMc02432)	443:519	Here we show that the seven-transmembrane receptor (7TMR) protein RgsP (SMc00074), together with the putative membrane-anchored peptidoglycan metallopeptidase RgsM (SMc02432), have key roles in unipolar peptidoglycan formation during growth and at mid-cell during cell division in Sinorhizobium meliloti.
30102748	1	1	theme	other	319:323	arg1	bacteria					336:343	many other rod-shaped bacteria	314:343	many other rod-shaped bacteria	314:343	Members of the Rhizobiales (class of α-proteobacteria) display zonal peptidoglycan cell wall growth at one cell pole, contrasting with the dispersed mode of cell wall growth along the sidewalls of many other rod-shaped bacteria.
30102748	2	2	dep	together	429:436	arg1	with					438:441	with	438:441	with	438:441	Here we show that the seven-transmembrane receptor (7TMR) protein RgsP (SMc00074), together with the putative membrane-anchored peptidoglycan metallopeptidase RgsM (SMc02432), have key roles in unipolar peptidoglycan formation during growth and at mid-cell during cell division in Sinorhizobium meliloti.
30102748	5	3	theme	cell	1039:1042	arg1	site					1052:1055	cell divison site	1039:1055	cell divison site	1039:1055	RgsP and RgsM localize to sites of zonal cell wall synthesis at the new cell pole and cell divison site, suggesting a role in cell wall biogenesis.
30102748	8	4	theme	wall	1405:1408	arg1	biogenesis/remodeling					1410:1430	cell wall biogenesis/remodeling	1400:1430	cell wall biogenesis/remodeling within the Rhizobiales	1400:1453	RgsP and RgsM orthologs are functional when interchanged between α-rhizobial species pointing to a conserved mechanism for cell wall biogenesis/remodeling within the Rhizobiales.
30102748	0	5	theme	unipolar	85:92	arg1	growth					94:99	unipolar growth	85:99	unipolar growth in Rhizobiales	85:114	Seven-transmembrane receptor protein RgsP and cell wall-binding protein RgsM promote unipolar growth in Rhizobiales.
30102748	1	6	theme	rod-shaped	325:334	arg1	bacteria					336:343	many other rod-shaped bacteria	314:343	many other rod-shaped bacteria	314:343	Members of the Rhizobiales (class of α-proteobacteria) display zonal peptidoglycan cell wall growth at one cell pole, contrasting with the dispersed mode of cell wall growth along the sidewalls of many other rod-shaped bacteria.
30102748	4	7	theme	messenger	856:864	arg1	player					898:903	a key regulatory player	881:903	a key regulatory player in the transition between bacterial lifestyles	881:950	The EAL domain confers phosphodiesterase activity towards the second messenger cyclic di-GMP, a key regulatory player in the transition between bacterial lifestyles.
30102748	4	7	theme	messenger	856:864	arg1	di-GMP					873:878	the second messenger cyclic di-GMP	845:878	the second messenger cyclic di-GMP	845:878	The EAL domain confers phosphodiesterase activity towards the second messenger cyclic di-GMP, a key regulatory player in the transition between bacterial lifestyles.
30102748	8	8	theme	RgsP	1277:1280	arg1	orthologs					1291:1299	RgsP and RgsM orthologs	1277:1299	RgsP and RgsM orthologs	1277:1299	RgsP and RgsM orthologs are functional when interchanged between α-rhizobial species pointing to a conserved mechanism for cell wall biogenesis/remodeling within the Rhizobiales.
30102748	4	9	theme	cyclic	866:871	arg1	player					898:903	a key regulatory player	881:903	a key regulatory player in the transition between bacterial lifestyles	881:950	The EAL domain confers phosphodiesterase activity towards the second messenger cyclic di-GMP, a key regulatory player in the transition between bacterial lifestyles.
30102748	4	9	theme	cyclic	866:871	arg1	di-GMP					873:878	the second messenger cyclic di-GMP	845:878	the second messenger cyclic di-GMP	845:878	The EAL domain confers phosphodiesterase activity towards the second messenger cyclic di-GMP, a key regulatory player in the transition between bacterial lifestyles.
30102748	1	10	theme	bacteria	336:343	arg1	sidewalls					301:309	the sidewalls	297:309	the sidewalls of many other rod-shaped bacteria	297:343	Members of the Rhizobiales (class of α-proteobacteria) display zonal peptidoglycan cell wall growth at one cell pole, contrasting with the dispersed mode of cell wall growth along the sidewalls of many other rod-shaped bacteria.
30102748	5	11	theme	cell	1079:1082	arg1	biogenesis					1089:1098	cell wall biogenesis	1079:1098	cell wall biogenesis	1079:1098	RgsP and RgsM localize to sites of zonal cell wall synthesis at the new cell pole and cell divison site, suggesting a role in cell wall biogenesis.
30102748	5	12	theme	synthesis	1004:1012	arg1	synthesis					1004:1012	zonal cell wall synthesis	988:1012	zonal cell wall synthesis	988:1012	RgsP and RgsM localize to sites of zonal cell wall synthesis at the new cell pole and cell divison site, suggesting a role in cell wall biogenesis.
30102748	5	12	theme	synthesis	1004:1012	arg1	sites					979:983	sites	979:983	sites of zonal cell wall synthesis	979:1012	RgsP and RgsM localize to sites of zonal cell wall synthesis at the new cell pole and cell divison site, suggesting a role in cell wall biogenesis.
30102748	6	13	theme	wall	1141:1144	arg1	biogenesis					1146:1155	cell wall biogenesis	1136:1155	cell wall biogenesis	1136:1155	The two proteins are essential for cell wall biogenesis and cell growth.
30102748	9	14	theme	biogenesis	1556:1565	arg1	regulation					1523:1532	the regulation	1519:1532	the regulation of unipolar cell wall biogenesis in α-rhizobia	1519:1579	Overall, our findings suggest that RgsP and RgsM contribute to the regulation of unipolar cell wall biogenesis in α-rhizobia.
30102748	1	15	theme	dispersed	256:264	arg1	mode					266:269	the dispersed mode	252:269	the dispersed mode of cell wall growth along the sidewalls of many other rod-shaped bacteria	252:343	Members of the Rhizobiales (class of α-proteobacteria) display zonal peptidoglycan cell wall growth at one cell pole, contrasting with the dispersed mode of cell wall growth along the sidewalls of many other rod-shaped bacteria.
30102748	4	16	theme	bacterial	931:939	arg1	lifestyles					941:950	bacterial lifestyles	931:950	bacterial lifestyles	931:950	The EAL domain confers phosphodiesterase activity towards the second messenger cyclic di-GMP, a key regulatory player in the transition between bacterial lifestyles.
30102748	8	17	theme	RgsM	1286:1289	arg1	orthologs					1291:1299	RgsP and RgsM orthologs	1277:1299	RgsP and RgsM orthologs	1277:1299	RgsP and RgsM orthologs are functional when interchanged between α-rhizobial species pointing to a conserved mechanism for cell wall biogenesis/remodeling within the Rhizobiales.
30102748	6	18	theme	cell	1136:1139	arg1	biogenesis					1146:1155	cell wall biogenesis	1136:1155	cell wall biogenesis	1136:1155	The two proteins are essential for cell wall biogenesis and cell growth.
30102748	3	19	theme	membrane-spanning	717:733	arg1	region					735:740	a membrane-spanning region	715:740	a membrane-spanning region	715:740	RgsP is composed of a periplasmic globular 7TMR-DISMED2 domain, a membrane-spanning region, and cytoplasmic PAS, GGDEF and EAL domains.
30102748	2	20	contain	have	522:525	arg1	SMc00074					418:425	SMc00074	418:425	SMc00074	418:425	Here we show that the seven-transmembrane receptor (7TMR) protein RgsP (SMc00074), together with the putative membrane-anchored peptidoglycan metallopeptidase RgsM (SMc02432), have key roles in unipolar peptidoglycan formation during growth and at mid-cell during cell division in Sinorhizobium meliloti.
30102748	2	20	contain	have	522:525	arg1	SMc02432					511:518	SMc02432	511:518	SMc02432	511:518	Here we show that the seven-transmembrane receptor (7TMR) protein RgsP (SMc00074), together with the putative membrane-anchored peptidoglycan metallopeptidase RgsM (SMc02432), have key roles in unipolar peptidoglycan formation during growth and at mid-cell during cell division in Sinorhizobium meliloti.
30102748	2	20	contain	have	522:525	arg1	RgsP					412:415	the seven-transmembrane receptor (7TMR) protein RgsP	364:415	the seven-transmembrane receptor (7TMR) protein RgsP (SMc00074)	364:426	Here we show that the seven-transmembrane receptor (7TMR) protein RgsP (SMc00074), together with the putative membrane-anchored peptidoglycan metallopeptidase RgsM (SMc02432), have key roles in unipolar peptidoglycan formation during growth and at mid-cell during cell division in Sinorhizobium meliloti.
30102748	2	20	contain	have	522:525	arg2	roles					531:535	key roles	527:535	key roles in unipolar peptidoglycan formation	527:571	Here we show that the seven-transmembrane receptor (7TMR) protein RgsP (SMc00074), together with the putative membrane-anchored peptidoglycan metallopeptidase RgsM (SMc02432), have key roles in unipolar peptidoglycan formation during growth and at mid-cell during cell division in Sinorhizobium meliloti.
30102748	2	20	contain	have	522:525	arg1	RgsM					505:508	the putative membrane-anchored peptidoglycan metallopeptidase RgsM	443:508	the putative membrane-anchored peptidoglycan metallopeptidase RgsM (SMc02432)	443:519	Here we show that the seven-transmembrane receptor (7TMR) protein RgsP (SMc00074), together with the putative membrane-anchored peptidoglycan metallopeptidase RgsM (SMc02432), have key roles in unipolar peptidoglycan formation during growth and at mid-cell during cell division in Sinorhizobium meliloti.
30102748	2	21	theme	receptor	388:395	arg1	SMc00074					418:425	SMc00074	418:425	SMc00074	418:425	Here we show that the seven-transmembrane receptor (7TMR) protein RgsP (SMc00074), together with the putative membrane-anchored peptidoglycan metallopeptidase RgsM (SMc02432), have key roles in unipolar peptidoglycan formation during growth and at mid-cell during cell division in Sinorhizobium meliloti.
30102748	2	21	theme	receptor	388:395	arg1	RgsP					412:415	the seven-transmembrane receptor (7TMR) protein RgsP	364:415	the seven-transmembrane receptor (7TMR) protein RgsP (SMc00074)	364:426	Here we show that the seven-transmembrane receptor (7TMR) protein RgsP (SMc00074), together with the putative membrane-anchored peptidoglycan metallopeptidase RgsM (SMc02432), have key roles in unipolar peptidoglycan formation during growth and at mid-cell during cell division in Sinorhizobium meliloti.
30102748	2	22	theme	cell	610:613	arg1	division					615:622	cell division	610:622	cell division in Sinorhizobium meliloti	610:648	Here we show that the seven-transmembrane receptor (7TMR) protein RgsP (SMc00074), together with the putative membrane-anchored peptidoglycan metallopeptidase RgsM (SMc02432), have key roles in unipolar peptidoglycan formation during growth and at mid-cell during cell division in Sinorhizobium meliloti.
30102748	7	23	dep	composition	1232:1242	arg1	binds					1253:1257	binds	1253:1257	binds to peptidoglycan	1253:1274	Cells depleted of RgsP or RgsM had an altered muropeptide composition and RgsM binds to peptidoglycan.
30102748	1	24	theme	zonal	180:184	arg1	growth					210:215	zonal peptidoglycan cell wall growth	180:215	zonal peptidoglycan cell wall growth	180:215	Members of the Rhizobiales (class of α-proteobacteria) display zonal peptidoglycan cell wall growth at one cell pole, contrasting with the dispersed mode of cell wall growth along the sidewalls of many other rod-shaped bacteria.
30102748	0	25	theme	receptor	20:27	arg1	RgsP					37:40	Seven-transmembrane receptor protein RgsP	0:40	Seven-transmembrane receptor protein RgsP	0:40	Seven-transmembrane receptor protein RgsP and cell wall-binding protein RgsM promote unipolar growth in Rhizobiales.
30102748	2	26	theme	putative	447:454	arg1	SMc02432					511:518	SMc02432	511:518	SMc02432	511:518	Here we show that the seven-transmembrane receptor (7TMR) protein RgsP (SMc00074), together with the putative membrane-anchored peptidoglycan metallopeptidase RgsM (SMc02432), have key roles in unipolar peptidoglycan formation during growth and at mid-cell during cell division in Sinorhizobium meliloti.
30102748	2	26	theme	putative	447:454	arg1	RgsM					505:508	the putative membrane-anchored peptidoglycan metallopeptidase RgsM	443:508	the putative membrane-anchored peptidoglycan metallopeptidase RgsM (SMc02432)	443:519	Here we show that the seven-transmembrane receptor (7TMR) protein RgsP (SMc00074), together with the putative membrane-anchored peptidoglycan metallopeptidase RgsM (SMc02432), have key roles in unipolar peptidoglycan formation during growth and at mid-cell during cell division in Sinorhizobium meliloti.
30102748	4	27	theme	second	849:854	arg1	player					898:903	a key regulatory player	881:903	a key regulatory player in the transition between bacterial lifestyles	881:950	The EAL domain confers phosphodiesterase activity towards the second messenger cyclic di-GMP, a key regulatory player in the transition between bacterial lifestyles.
30102748	4	27	theme	second	849:854	arg1	di-GMP					873:878	the second messenger cyclic di-GMP	845:878	the second messenger cyclic di-GMP	845:878	The EAL domain confers phosphodiesterase activity towards the second messenger cyclic di-GMP, a key regulatory player in the transition between bacterial lifestyles.
30102748	5	28	theme	zonal	988:992	arg1	synthesis					1004:1012	zonal cell wall synthesis	988:1012	zonal cell wall synthesis	988:1012	RgsP and RgsM localize to sites of zonal cell wall synthesis at the new cell pole and cell divison site, suggesting a role in cell wall biogenesis.
30102748	9	29	theme	unipolar	1537:1544	arg1	biogenesis					1556:1565	unipolar cell wall biogenesis	1537:1565	unipolar cell wall biogenesis	1537:1565	Overall, our findings suggest that RgsP and RgsM contribute to the regulation of unipolar cell wall biogenesis in α-rhizobia.
30102748	1	30	theme	peptidoglycan	186:198	arg1	growth					210:215	zonal peptidoglycan cell wall growth	180:215	zonal peptidoglycan cell wall growth	180:215	Members of the Rhizobiales (class of α-proteobacteria) display zonal peptidoglycan cell wall growth at one cell pole, contrasting with the dispersed mode of cell wall growth along the sidewalls of many other rod-shaped bacteria.
30102748	0	31	theme	Seven-transmembrane	0:18	arg1	RgsP					37:40	Seven-transmembrane receptor protein RgsP	0:40	Seven-transmembrane receptor protein RgsP	0:40	Seven-transmembrane receptor protein RgsP and cell wall-binding protein RgsM promote unipolar growth in Rhizobiales.
30102748	7	32	contain	had	1205:1207	arg2	composition					1232:1242	an altered muropeptide composition	1209:1242	an altered muropeptide composition	1209:1242	Cells depleted of RgsP or RgsM had an altered muropeptide composition and RgsM binds to peptidoglycan.
30102748	7	32	contain	had	1205:1207	arg1	Cells					1174:1178	Cells	1174:1178	Cells depleted of RgsP or RgsM	1174:1203	Cells depleted of RgsP or RgsM had an altered muropeptide composition and RgsM binds to peptidoglycan.
30102748	7	32	contain	had	1205:1207	arg2	RgsM					1248:1251	RgsM	1248:1251	RgsM	1248:1251	Cells depleted of RgsP or RgsM had an altered muropeptide composition and RgsM binds to peptidoglycan.
30102748	5	33	theme	new	1021:1023	arg1	pole					1030:1033	the new cell pole	1017:1033	the new cell pole	1017:1033	RgsP and RgsM localize to sites of zonal cell wall synthesis at the new cell pole and cell divison site, suggesting a role in cell wall biogenesis.
30102748	8	34	theme	conserved	1376:1384	arg1	mechanism					1386:1394	a conserved mechanism	1374:1394	a conserved mechanism for cell wall biogenesis/remodeling within the Rhizobiales	1374:1453	RgsP and RgsM orthologs are functional when interchanged between α-rhizobial species pointing to a conserved mechanism for cell wall biogenesis/remodeling within the Rhizobiales.
30102748	1	35	theme	cell	200:203	arg1	growth					210:215	zonal peptidoglycan cell wall growth	180:215	zonal peptidoglycan cell wall growth	180:215	Members of the Rhizobiales (class of α-proteobacteria) display zonal peptidoglycan cell wall growth at one cell pole, contrasting with the dispersed mode of cell wall growth along the sidewalls of many other rod-shaped bacteria.
30102748	1	36	theme	cell	274:277	arg1	growth					284:289	cell wall growth	274:289	cell wall growth	274:289	Members of the Rhizobiales (class of α-proteobacteria) display zonal peptidoglycan cell wall growth at one cell pole, contrasting with the dispersed mode of cell wall growth along the sidewalls of many other rod-shaped bacteria.
30102748	8	37	theme	α-rhizobial	1342:1352	arg1	species					1354:1360	α-rhizobial species	1342:1360	α-rhizobial species pointing to a conserved mechanism for cell wall biogenesis/remodeling within the Rhizobiales	1342:1453	RgsP and RgsM orthologs are functional when interchanged between α-rhizobial species pointing to a conserved mechanism for cell wall biogenesis/remodeling within the Rhizobiales.
30102748	1	38	theme	wall	205:208	arg1	growth					210:215	zonal peptidoglycan cell wall growth	180:215	zonal peptidoglycan cell wall growth	180:215	Members of the Rhizobiales (class of α-proteobacteria) display zonal peptidoglycan cell wall growth at one cell pole, contrasting with the dispersed mode of cell wall growth along the sidewalls of many other rod-shaped bacteria.
30102748	0	39	theme	protein	29:35	arg1	RgsP					37:40	Seven-transmembrane receptor protein RgsP	0:40	Seven-transmembrane receptor protein RgsP	0:40	Seven-transmembrane receptor protein RgsP and cell wall-binding protein RgsM promote unipolar growth in Rhizobiales.
30102748	1	40	theme	wall	279:282	arg1	growth					284:289	cell wall growth	274:289	cell wall growth	274:289	Members of the Rhizobiales (class of α-proteobacteria) display zonal peptidoglycan cell wall growth at one cell pole, contrasting with the dispersed mode of cell wall growth along the sidewalls of many other rod-shaped bacteria.
30102748	2	41	theme	key	527:529	arg1	roles					531:535	key roles	527:535	key roles in unipolar peptidoglycan formation	527:571	Here we show that the seven-transmembrane receptor (7TMR) protein RgsP (SMc00074), together with the putative membrane-anchored peptidoglycan metallopeptidase RgsM (SMc02432), have key roles in unipolar peptidoglycan formation during growth and at mid-cell during cell division in Sinorhizobium meliloti.
30102748	0	42	from	growth	94:99	arg1	Rhizobiales					104:114	Rhizobiales	104:114	Rhizobiales	104:114	Seven-transmembrane receptor protein RgsP and cell wall-binding protein RgsM promote unipolar growth in Rhizobiales.
30102748	9	43	theme	cell	1546:1549	arg1	biogenesis					1556:1565	unipolar cell wall biogenesis	1537:1565	unipolar cell wall biogenesis	1537:1565	Overall, our findings suggest that RgsP and RgsM contribute to the regulation of unipolar cell wall biogenesis in α-rhizobia.
30102748	8	44	theme	cell	1400:1403	arg1	biogenesis/remodeling					1410:1430	cell wall biogenesis/remodeling	1400:1430	cell wall biogenesis/remodeling within the Rhizobiales	1400:1453	RgsP and RgsM orthologs are functional when interchanged between α-rhizobial species pointing to a conserved mechanism for cell wall biogenesis/remodeling within the Rhizobiales.
30102748	0	45	theme	cell	46:49	arg1	RgsM					72:75	cell wall-binding protein RgsM	46:75	cell wall-binding protein RgsM	46:75	Seven-transmembrane receptor protein RgsP and cell wall-binding protein RgsM promote unipolar growth in Rhizobiales.
30102748	1	46	theme	growth	284:289	arg1	mode					266:269	the dispersed mode	252:269	the dispersed mode of cell wall growth along the sidewalls of many other rod-shaped bacteria	252:343	Members of the Rhizobiales (class of α-proteobacteria) display zonal peptidoglycan cell wall growth at one cell pole, contrasting with the dispersed mode of cell wall growth along the sidewalls of many other rod-shaped bacteria.
30102748	5	47	theme	divison	1044:1050	arg1	site					1052:1055	cell divison site	1039:1055	cell divison site	1039:1055	RgsP and RgsM localize to sites of zonal cell wall synthesis at the new cell pole and cell divison site, suggesting a role in cell wall biogenesis.
30102748	5	48	theme	wall	999:1002	arg1	synthesis					1004:1012	zonal cell wall synthesis	988:1012	zonal cell wall synthesis	988:1012	RgsP and RgsM localize to sites of zonal cell wall synthesis at the new cell pole and cell divison site, suggesting a role in cell wall biogenesis.
30102748	2	49	theme	7TMR	398:401	arg1	SMc00074					418:425	SMc00074	418:425	SMc00074	418:425	Here we show that the seven-transmembrane receptor (7TMR) protein RgsP (SMc00074), together with the putative membrane-anchored peptidoglycan metallopeptidase RgsM (SMc02432), have key roles in unipolar peptidoglycan formation during growth and at mid-cell during cell division in Sinorhizobium meliloti.
30102748	2	49	theme	7TMR	398:401	arg1	RgsP					412:415	the seven-transmembrane receptor (7TMR) protein RgsP	364:415	the seven-transmembrane receptor (7TMR) protein RgsP (SMc00074)	364:426	Here we show that the seven-transmembrane receptor (7TMR) protein RgsP (SMc00074), together with the putative membrane-anchored peptidoglycan metallopeptidase RgsM (SMc02432), have key roles in unipolar peptidoglycan formation during growth and at mid-cell during cell division in Sinorhizobium meliloti.
30102748	3	50	theme	GGDEF	764:768	arg1	domains					778:784	GGDEF and EAL domains	764:784	GGDEF and EAL domains	764:784	RgsP is composed of a periplasmic globular 7TMR-DISMED2 domain, a membrane-spanning region, and cytoplasmic PAS, GGDEF and EAL domains.
30102748	3	51	theme	EAL	774:776	arg1	domains					778:784	GGDEF and EAL domains	764:784	GGDEF and EAL domains	764:784	RgsP is composed of a periplasmic globular 7TMR-DISMED2 domain, a membrane-spanning region, and cytoplasmic PAS, GGDEF and EAL domains.
30102748	0	52	theme	protein	64:70	arg1	RgsM					72:75	cell wall-binding protein RgsM	46:75	cell wall-binding protein RgsM	46:75	Seven-transmembrane receptor protein RgsP and cell wall-binding protein RgsM promote unipolar growth in Rhizobiales.
30102748	5	53	theme	wall	1084:1087	arg1	biogenesis					1089:1098	cell wall biogenesis	1079:1098	cell wall biogenesis	1079:1098	RgsP and RgsM localize to sites of zonal cell wall synthesis at the new cell pole and cell divison site, suggesting a role in cell wall biogenesis.
30102748	4	54	theme	key	883:885	arg1	player					898:903	a key regulatory player	881:903	a key regulatory player in the transition between bacterial lifestyles	881:950	The EAL domain confers phosphodiesterase activity towards the second messenger cyclic di-GMP, a key regulatory player in the transition between bacterial lifestyles.
30102748	4	54	theme	key	883:885	arg1	di-GMP					873:878	the second messenger cyclic di-GMP	845:878	the second messenger cyclic di-GMP	845:878	The EAL domain confers phosphodiesterase activity towards the second messenger cyclic di-GMP, a key regulatory player in the transition between bacterial lifestyles.
30102748	3	55	theme	cytoplasmic	747:757	arg1	PAS					759:761	cytoplasmic PAS	747:761	cytoplasmic PAS	747:761	RgsP is composed of a periplasmic globular 7TMR-DISMED2 domain, a membrane-spanning region, and cytoplasmic PAS, GGDEF and EAL domains.
30102748	4	56	theme	phosphodiesterase	810:826	arg1	activity					828:835	phosphodiesterase activity	810:835	phosphodiesterase activity	810:835	The EAL domain confers phosphodiesterase activity towards the second messenger cyclic di-GMP, a key regulatory player in the transition between bacterial lifestyles.
30102748	7	57	theme	muropeptide	1220:1230	arg1	composition					1232:1242	an altered muropeptide composition	1209:1242	an altered muropeptide composition	1209:1242	Cells depleted of RgsP or RgsM had an altered muropeptide composition and RgsM binds to peptidoglycan.
30102748	0	58	theme	wall-binding	51:62	arg1	RgsM					72:75	cell wall-binding protein RgsM	46:75	cell wall-binding protein RgsM	46:75	Seven-transmembrane receptor protein RgsP and cell wall-binding protein RgsM promote unipolar growth in Rhizobiales.
30102748	2	59	from	division	615:622	arg1	meliloti					641:648	Sinorhizobium meliloti	627:648	Sinorhizobium meliloti	627:648	Here we show that the seven-transmembrane receptor (7TMR) protein RgsP (SMc00074), together with the putative membrane-anchored peptidoglycan metallopeptidase RgsM (SMc02432), have key roles in unipolar peptidoglycan formation during growth and at mid-cell during cell division in Sinorhizobium meliloti.
30102748	2	60	theme	peptidoglycan	549:561	arg1	formation					563:571	unipolar peptidoglycan formation	540:571	unipolar peptidoglycan formation	540:571	Here we show that the seven-transmembrane receptor (7TMR) protein RgsP (SMc00074), together with the putative membrane-anchored peptidoglycan metallopeptidase RgsM (SMc02432), have key roles in unipolar peptidoglycan formation during growth and at mid-cell during cell division in Sinorhizobium meliloti.
30102748	4	61	from	player	898:903	arg1	transition					912:921	the transition	908:921	the transition between bacterial lifestyles	908:950	The EAL domain confers phosphodiesterase activity towards the second messenger cyclic di-GMP, a key regulatory player in the transition between bacterial lifestyles.
30102748	3	62	theme	globular	685:692	arg1	domain					707:712	a periplasmic globular 7TMR-DISMED2 domain	671:712	a periplasmic globular 7TMR-DISMED2 domain	671:712	RgsP is composed of a periplasmic globular 7TMR-DISMED2 domain, a membrane-spanning region, and cytoplasmic PAS, GGDEF and EAL domains.
30102748	5	63	from	role	1071:1074	arg1	biogenesis					1089:1098	cell wall biogenesis	1079:1098	cell wall biogenesis	1079:1098	RgsP and RgsM localize to sites of zonal cell wall synthesis at the new cell pole and cell divison site, suggesting a role in cell wall biogenesis.
30102748	2	64	theme	unipolar	540:547	arg1	formation					563:571	unipolar peptidoglycan formation	540:571	unipolar peptidoglycan formation	540:571	Here we show that the seven-transmembrane receptor (7TMR) protein RgsP (SMc00074), together with the putative membrane-anchored peptidoglycan metallopeptidase RgsM (SMc02432), have key roles in unipolar peptidoglycan formation during growth and at mid-cell during cell division in Sinorhizobium meliloti.
30102748	5	65	theme	cell	1025:1028	arg1	pole					1030:1033	the new cell pole	1017:1033	the new cell pole	1017:1033	RgsP and RgsM localize to sites of zonal cell wall synthesis at the new cell pole and cell divison site, suggesting a role in cell wall biogenesis.
30102748	4	66	theme	EAL	791:793	arg1	domain					795:800	The EAL domain	787:800	The EAL domain	787:800	The EAL domain confers phosphodiesterase activity towards the second messenger cyclic di-GMP, a key regulatory player in the transition between bacterial lifestyles.
30102748	1	67	theme	Rhizobiales	132:142	arg1	Members					117:123	Members	117:123	Members of the Rhizobiales (class of α-proteobacteria)	117:170	Members of the Rhizobiales (class of α-proteobacteria) display zonal peptidoglycan cell wall growth at one cell pole, contrasting with the dispersed mode of cell wall growth along the sidewalls of many other rod-shaped bacteria.
30102748	9	68	theme	wall	1551:1554	arg1	biogenesis					1556:1565	unipolar cell wall biogenesis	1537:1565	unipolar cell wall biogenesis	1537:1565	Overall, our findings suggest that RgsP and RgsM contribute to the regulation of unipolar cell wall biogenesis in α-rhizobia.
30102748	1	69	theme	cell	224:227	arg1	pole					229:232	one cell pole	220:232	one cell pole	220:232	Members of the Rhizobiales (class of α-proteobacteria) display zonal peptidoglycan cell wall growth at one cell pole, contrasting with the dispersed mode of cell wall growth along the sidewalls of many other rod-shaped bacteria.
30102748	4	70	theme	regulatory	887:896	arg1	player					898:903	a key regulatory player	881:903	a key regulatory player in the transition between bacterial lifestyles	881:950	The EAL domain confers phosphodiesterase activity towards the second messenger cyclic di-GMP, a key regulatory player in the transition between bacterial lifestyles.
30102748	4	70	theme	regulatory	887:896	arg1	di-GMP					873:878	the second messenger cyclic di-GMP	845:878	the second messenger cyclic di-GMP	845:878	The EAL domain confers phosphodiesterase activity towards the second messenger cyclic di-GMP, a key regulatory player in the transition between bacterial lifestyles.
30102748	6	71	theme	cell	1161:1164	arg1	growth					1166:1171	cell growth	1161:1171	cell growth	1161:1171	The two proteins are essential for cell wall biogenesis and cell growth.
30102748	7	72	theme	altered	1212:1218	arg1	composition					1232:1242	an altered muropeptide composition	1209:1242	an altered muropeptide composition	1209:1242	Cells depleted of RgsP or RgsM had an altered muropeptide composition and RgsM binds to peptidoglycan.
30102748	2	73	theme	metallopeptidase	488:503	arg1	SMc02432					511:518	SMc02432	511:518	SMc02432	511:518	Here we show that the seven-transmembrane receptor (7TMR) protein RgsP (SMc00074), together with the putative membrane-anchored peptidoglycan metallopeptidase RgsM (SMc02432), have key roles in unipolar peptidoglycan formation during growth and at mid-cell during cell division in Sinorhizobium meliloti.
30102748	2	73	theme	metallopeptidase	488:503	arg1	RgsM					505:508	the putative membrane-anchored peptidoglycan metallopeptidase RgsM	443:508	the putative membrane-anchored peptidoglycan metallopeptidase RgsM (SMc02432)	443:519	Here we show that the seven-transmembrane receptor (7TMR) protein RgsP (SMc00074), together with the putative membrane-anchored peptidoglycan metallopeptidase RgsM (SMc02432), have key roles in unipolar peptidoglycan formation during growth and at mid-cell during cell division in Sinorhizobium meliloti.
30102748	3	74	theme	periplasmic	673:683	arg1	domain					707:712	a periplasmic globular 7TMR-DISMED2 domain	671:712	a periplasmic globular 7TMR-DISMED2 domain	671:712	RgsP is composed of a periplasmic globular 7TMR-DISMED2 domain, a membrane-spanning region, and cytoplasmic PAS, GGDEF and EAL domains.
30102748	2	75	theme	protein	404:410	arg1	SMc00074					418:425	SMc00074	418:425	SMc00074	418:425	Here we show that the seven-transmembrane receptor (7TMR) protein RgsP (SMc00074), together with the putative membrane-anchored peptidoglycan metallopeptidase RgsM (SMc02432), have key roles in unipolar peptidoglycan formation during growth and at mid-cell during cell division in Sinorhizobium meliloti.
30102748	2	75	theme	protein	404:410	arg1	RgsP					412:415	the seven-transmembrane receptor (7TMR) protein RgsP	364:415	the seven-transmembrane receptor (7TMR) protein RgsP (SMc00074)	364:426	Here we show that the seven-transmembrane receptor (7TMR) protein RgsP (SMc00074), together with the putative membrane-anchored peptidoglycan metallopeptidase RgsM (SMc02432), have key roles in unipolar peptidoglycan formation during growth and at mid-cell during cell division in Sinorhizobium meliloti.
30102748	1	76	theme	class	145:149	arg1	Rhizobiales					132:142	the Rhizobiales	128:142	the Rhizobiales (class of α-proteobacteria)	128:170	Members of the Rhizobiales (class of α-proteobacteria) display zonal peptidoglycan cell wall growth at one cell pole, contrasting with the dispersed mode of cell wall growth along the sidewalls of many other rod-shaped bacteria.
30102748	1	76	theme	class	145:149	arg1	α-proteobacteria					154:169	class of α-proteobacteria	145:169	class of α-proteobacteria	145:169	Members of the Rhizobiales (class of α-proteobacteria) display zonal peptidoglycan cell wall growth at one cell pole, contrasting with the dispersed mode of cell wall growth along the sidewalls of many other rod-shaped bacteria.
30102748	2	77	theme	seven-transmembrane	368:386	arg1	SMc00074					418:425	SMc00074	418:425	SMc00074	418:425	Here we show that the seven-transmembrane receptor (7TMR) protein RgsP (SMc00074), together with the putative membrane-anchored peptidoglycan metallopeptidase RgsM (SMc02432), have key roles in unipolar peptidoglycan formation during growth and at mid-cell during cell division in Sinorhizobium meliloti.
30102748	2	77	theme	seven-transmembrane	368:386	arg1	RgsP					412:415	the seven-transmembrane receptor (7TMR) protein RgsP	364:415	the seven-transmembrane receptor (7TMR) protein RgsP (SMc00074)	364:426	Here we show that the seven-transmembrane receptor (7TMR) protein RgsP (SMc00074), together with the putative membrane-anchored peptidoglycan metallopeptidase RgsM (SMc02432), have key roles in unipolar peptidoglycan formation during growth and at mid-cell during cell division in Sinorhizobium meliloti.
30102748	2	78	from	roles	531:535	arg1	formation					563:571	unipolar peptidoglycan formation	540:571	unipolar peptidoglycan formation	540:571	Here we show that the seven-transmembrane receptor (7TMR) protein RgsP (SMc00074), together with the putative membrane-anchored peptidoglycan metallopeptidase RgsM (SMc02432), have key roles in unipolar peptidoglycan formation during growth and at mid-cell during cell division in Sinorhizobium meliloti.
30102748	2	79	theme	peptidoglycan	474:486	arg1	SMc02432					511:518	SMc02432	511:518	SMc02432	511:518	Here we show that the seven-transmembrane receptor (7TMR) protein RgsP (SMc00074), together with the putative membrane-anchored peptidoglycan metallopeptidase RgsM (SMc02432), have key roles in unipolar peptidoglycan formation during growth and at mid-cell during cell division in Sinorhizobium meliloti.
30102748	2	79	theme	peptidoglycan	474:486	arg1	RgsM					505:508	the putative membrane-anchored peptidoglycan metallopeptidase RgsM	443:508	the putative membrane-anchored peptidoglycan metallopeptidase RgsM (SMc02432)	443:519	Here we show that the seven-transmembrane receptor (7TMR) protein RgsP (SMc00074), together with the putative membrane-anchored peptidoglycan metallopeptidase RgsM (SMc02432), have key roles in unipolar peptidoglycan formation during growth and at mid-cell during cell division in Sinorhizobium meliloti.
30102748	1	80	theme	many	314:317	arg1	bacteria					336:343	many other rod-shaped bacteria	314:343	many other rod-shaped bacteria	314:343	Members of the Rhizobiales (class of α-proteobacteria) display zonal peptidoglycan cell wall growth at one cell pole, contrasting with the dispersed mode of cell wall growth along the sidewalls of many other rod-shaped bacteria.
30102748	5	81	theme	cell	994:997	arg1	synthesis					1004:1012	zonal cell wall synthesis	988:1012	zonal cell wall synthesis	988:1012	RgsP and RgsM localize to sites of zonal cell wall synthesis at the new cell pole and cell divison site, suggesting a role in cell wall biogenesis.
30102748	3	82	theme	7TMR-DISMED2	694:705	arg1	domain					707:712	a periplasmic globular 7TMR-DISMED2 domain	671:712	a periplasmic globular 7TMR-DISMED2 domain	671:712	RgsP is composed of a periplasmic globular 7TMR-DISMED2 domain, a membrane-spanning region, and cytoplasmic PAS, GGDEF and EAL domains.
30102748	1	83	theme	of	151:152	arg1	Rhizobiales					132:142	the Rhizobiales	128:142	the Rhizobiales (class of α-proteobacteria)	128:170	Members of the Rhizobiales (class of α-proteobacteria) display zonal peptidoglycan cell wall growth at one cell pole, contrasting with the dispersed mode of cell wall growth along the sidewalls of many other rod-shaped bacteria.
30102748	1	83	theme	of	151:152	arg1	α-proteobacteria					154:169	class of α-proteobacteria	145:169	class of α-proteobacteria	145:169	Members of the Rhizobiales (class of α-proteobacteria) display zonal peptidoglycan cell wall growth at one cell pole, contrasting with the dispersed mode of cell wall growth along the sidewalls of many other rod-shaped bacteria.
30102748	9	84	from	regulation	1523:1532	arg1	α-rhizobia					1570:1579	α-rhizobia	1570:1579	α-rhizobia	1570:1579	Overall, our findings suggest that RgsP and RgsM contribute to the regulation of unipolar cell wall biogenesis in α-rhizobia.
31358565	0	0	theme	colitis-associated	72:89	arg1	cancer					102:107	colitis-associated colorectal cancer	72:107	colitis-associated colorectal cancer	72:107	Editing of the gut microbiota reduces carcinogenesis in mouse models of colitis-associated colorectal cancer.
31358565	2	1	theme	microbiota	346:355	arg1	metabolism					357:366	gut microbiota metabolism	342:366	gut microbiota metabolism	342:366	Here, we sought to determine whether precision editing of gut microbiota metabolism and composition could decrease the risk for tumor development in mouse models of colitis-associated colorectal cancer (CAC).
31358565	3	2	from	strains	540:546	arg1	model					599:603	the azoxymethane/dextran sulfate sodium colitis model	551:603	the azoxymethane/dextran sulfate sodium colitis model	551:603	Expansion of experimentally introduced E. coli strains in the azoxymethane/dextran sulfate sodium colitis model was driven by molybdoenzyme-dependent metabolic pathways.
31358565	5	3	theme	sulfate	1011:1017	arg1	model					1034:1038	the azoxymethane/dextran sulfate sodium colitis model	986:1038	the azoxymethane/dextran sulfate sodium colitis model	986:1038	Restricting the bloom of Enterobacteriaceae decreased intestinal inflammation and reduced the incidence of colonic tumors in two models of CAC, the azoxymethane/dextran sulfate sodium colitis model and azoxymethane-treated, Il10-deficient mice.
31358565	4	4	with	colonization	766:777	arg1	coli					807:810	genotoxin-producing E. coli	784:810	genotoxin-producing E. coli	784:810	Oral administration of sodium tungstate inhibited E. coli molybdoenzymes and selectively decreased gut colonization with genotoxin-producing E. coli and other Enterobacteriaceae.
31358565	2	5	theme	gut	342:344	arg1	metabolism					357:366	gut microbiota metabolism	342:366	gut microbiota metabolism	342:366	Here, we sought to determine whether precision editing of gut microbiota metabolism and composition could decrease the risk for tumor development in mouse models of colitis-associated colorectal cancer (CAC).
31358565	6	6	theme	metabolic	1104:1112	arg1	strategy					1199:1206	a suitable strategy	1188:1206	a suitable strategy to prevent the development of malignancies arising from gut microbiota dysbiosis	1188:1287	We conclude that metabolic targeting of protumoral Enterobacteriaceae during chronic inflammation is a suitable strategy to prevent the development of malignancies arising from gut microbiota dysbiosis.
31358565	6	6	theme	metabolic	1104:1112	arg1	targeting					1114:1122	metabolic targeting	1104:1122	metabolic targeting of protumoral Enterobacteriaceae during chronic inflammation	1104:1183	We conclude that metabolic targeting of protumoral Enterobacteriaceae during chronic inflammation is a suitable strategy to prevent the development of malignancies arising from gut microbiota dysbiosis.
31358565	0	7	theme	cancer	102:107	arg1	models					62:67	mouse models	56:67	mouse models of colitis-associated colorectal cancer	56:107	Editing of the gut microbiota reduces carcinogenesis in mouse models of colitis-associated colorectal cancer.
31358565	5	8	theme	CAC	981:983	arg1	mice					1081:1084	azoxymethane-treated, Il10-deficient mice	1044:1084	azoxymethane-treated, Il10-deficient mice	1044:1084	Restricting the bloom of Enterobacteriaceae decreased intestinal inflammation and reduced the incidence of colonic tumors in two models of CAC, the azoxymethane/dextran sulfate sodium colitis model and azoxymethane-treated, Il10-deficient mice.
31358565	5	8	theme	CAC	981:983	arg1	model					1034:1038	the azoxymethane/dextran sulfate sodium colitis model	986:1038	the azoxymethane/dextran sulfate sodium colitis model	986:1038	Restricting the bloom of Enterobacteriaceae decreased intestinal inflammation and reduced the incidence of colonic tumors in two models of CAC, the azoxymethane/dextran sulfate sodium colitis model and azoxymethane-treated, Il10-deficient mice.
31358565	5	8	theme	CAC	981:983	arg1	models					971:976	two models	967:976	two models of CAC	967:983	Restricting the bloom of Enterobacteriaceae decreased intestinal inflammation and reduced the incidence of colonic tumors in two models of CAC, the azoxymethane/dextran sulfate sodium colitis model and azoxymethane-treated, Il10-deficient mice.
31358565	3	9	theme	sulfate	576:582	arg1	model					599:603	the azoxymethane/dextran sulfate sodium colitis model	551:603	the azoxymethane/dextran sulfate sodium colitis model	551:603	Expansion of experimentally introduced E. coli strains in the azoxymethane/dextran sulfate sodium colitis model was driven by molybdoenzyme-dependent metabolic pathways.
31358565	0	10	theme	colorectal	91:100	arg1	cancer					102:107	colitis-associated colorectal cancer	72:107	colitis-associated colorectal cancer	72:107	Editing of the gut microbiota reduces carcinogenesis in mouse models of colitis-associated colorectal cancer.
31358565	5	11	theme	colitis	1026:1032	arg1	model					1034:1038	the azoxymethane/dextran sulfate sodium colitis model	986:1038	the azoxymethane/dextran sulfate sodium colitis model	986:1038	Restricting the bloom of Enterobacteriaceae decreased intestinal inflammation and reduced the incidence of colonic tumors in two models of CAC, the azoxymethane/dextran sulfate sodium colitis model and azoxymethane-treated, Il10-deficient mice.
31358565	1	12	from	dysbiosis	150:158	arg1	particular					164:173	particular	164:173	particular	164:173	Chronic inflammation and gut microbiota dysbiosis, in particular the bloom of genotoxin-producing E. coli strains, are risk factors for the development of colorectal cancer.
31358565	1	13	theme	colorectal	265:274	arg1	cancer					276:281	colorectal cancer	265:281	colorectal cancer	265:281	Chronic inflammation and gut microbiota dysbiosis, in particular the bloom of genotoxin-producing E. coli strains, are risk factors for the development of colorectal cancer.
31358565	6	14	theme	microbiota	1268:1277	arg1	dysbiosis					1279:1287	gut microbiota dysbiosis	1264:1287	gut microbiota dysbiosis	1264:1287	We conclude that metabolic targeting of protumoral Enterobacteriaceae during chronic inflammation is a suitable strategy to prevent the development of malignancies arising from gut microbiota dysbiosis.
31358565	4	15	theme	Oral	663:666	arg1	administration					668:681	Oral administration	663:681	Oral administration of sodium tungstate	663:701	Oral administration of sodium tungstate inhibited E. coli molybdoenzymes and selectively decreased gut colonization with genotoxin-producing E. coli and other Enterobacteriaceae.
31358565	3	16	theme	coli	535:538	arg1	strains					540:546	experimentally introduced E. coli strains	506:546	experimentally introduced E. coli strains in the azoxymethane/dextran sulfate sodium colitis model	506:603	Expansion of experimentally introduced E. coli strains in the azoxymethane/dextran sulfate sodium colitis model was driven by molybdoenzyme-dependent metabolic pathways.
31358565	5	17	theme	sodium	1019:1024	arg1	model					1034:1038	the azoxymethane/dextran sulfate sodium colitis model	986:1038	the azoxymethane/dextran sulfate sodium colitis model	986:1038	Restricting the bloom of Enterobacteriaceae decreased intestinal inflammation and reduced the incidence of colonic tumors in two models of CAC, the azoxymethane/dextran sulfate sodium colitis model and azoxymethane-treated, Il10-deficient mice.
31358565	5	18	theme	tumors	957:962	arg1	incidence					936:944	the incidence	932:944	the incidence of colonic tumors in two models of CAC, the azoxymethane/dextran sulfate sodium colitis model and azoxymethane-treated, Il10-deficient mice	932:1084	Restricting the bloom of Enterobacteriaceae decreased intestinal inflammation and reduced the incidence of colonic tumors in two models of CAC, the azoxymethane/dextran sulfate sodium colitis model and azoxymethane-treated, Il10-deficient mice.
31358565	5	19	dep	azoxymethane-treated	1044:1063	arg1	Il10-deficient					1066:1079	Il10-deficient	1066:1079	Il10-deficient	1066:1079	Restricting the bloom of Enterobacteriaceae decreased intestinal inflammation and reduced the incidence of colonic tumors in two models of CAC, the azoxymethane/dextran sulfate sodium colitis model and azoxymethane-treated, Il10-deficient mice.
31358565	1	20	theme	risk	229:232	arg1	factors					234:240	risk factors	229:240	risk factors for the development of colorectal cancer	229:281	Chronic inflammation and gut microbiota dysbiosis, in particular the bloom of genotoxin-producing E. coli strains, are risk factors for the development of colorectal cancer.
31358565	1	20	theme	risk	229:232	arg1	inflammation					118:129	Chronic inflammation	110:129	Chronic inflammation	110:129	Chronic inflammation and gut microbiota dysbiosis, in particular the bloom of genotoxin-producing E. coli strains, are risk factors for the development of colorectal cancer.
31358565	1	20	theme	risk	229:232	arg1	dysbiosis					150:158	gut microbiota dysbiosis	135:158	gut microbiota dysbiosis	135:158	Chronic inflammation and gut microbiota dysbiosis, in particular the bloom of genotoxin-producing E. coli strains, are risk factors for the development of colorectal cancer.
31358565	4	21	theme	tungstate	693:701	arg1	administration					668:681	Oral administration	663:681	Oral administration of sodium tungstate	663:701	Oral administration of sodium tungstate inhibited E. coli molybdoenzymes and selectively decreased gut colonization with genotoxin-producing E. coli and other Enterobacteriaceae.
31358565	5	22	theme	intestinal	896:905	arg1	inflammation					907:918	intestinal inflammation	896:918	intestinal inflammation	896:918	Restricting the bloom of Enterobacteriaceae decreased intestinal inflammation and reduced the incidence of colonic tumors in two models of CAC, the azoxymethane/dextran sulfate sodium colitis model and azoxymethane-treated, Il10-deficient mice.
31358565	3	23	theme	E.	532:533	arg1	strains					540:546	experimentally introduced E. coli strains	506:546	experimentally introduced E. coli strains in the azoxymethane/dextran sulfate sodium colitis model	506:603	Expansion of experimentally introduced E. coli strains in the azoxymethane/dextran sulfate sodium colitis model was driven by molybdoenzyme-dependent metabolic pathways.
31358565	3	24	theme	sodium	584:589	arg1	model					599:603	the azoxymethane/dextran sulfate sodium colitis model	551:603	the azoxymethane/dextran sulfate sodium colitis model	551:603	Expansion of experimentally introduced E. coli strains in the azoxymethane/dextran sulfate sodium colitis model was driven by molybdoenzyme-dependent metabolic pathways.
31358565	6	25	theme	Enterobacteriaceae	1138:1155	arg1	strategy					1199:1206	a suitable strategy	1188:1206	a suitable strategy to prevent the development of malignancies arising from gut microbiota dysbiosis	1188:1287	We conclude that metabolic targeting of protumoral Enterobacteriaceae during chronic inflammation is a suitable strategy to prevent the development of malignancies arising from gut microbiota dysbiosis.
31358565	6	25	theme	Enterobacteriaceae	1138:1155	arg1	targeting					1114:1122	metabolic targeting	1104:1122	metabolic targeting of protumoral Enterobacteriaceae during chronic inflammation	1104:1183	We conclude that metabolic targeting of protumoral Enterobacteriaceae during chronic inflammation is a suitable strategy to prevent the development of malignancies arising from gut microbiota dysbiosis.
31358565	1	26	theme	cancer	276:281	arg1	development					250:260	the development	246:260	the development of colorectal cancer	246:281	Chronic inflammation and gut microbiota dysbiosis, in particular the bloom of genotoxin-producing E. coli strains, are risk factors for the development of colorectal cancer.
31358565	1	27	from	inflammation	118:129	arg1	particular					164:173	particular	164:173	particular	164:173	Chronic inflammation and gut microbiota dysbiosis, in particular the bloom of genotoxin-producing E. coli strains, are risk factors for the development of colorectal cancer.
31358565	1	28	dep	particular	164:173	arg1	bloom					179:183	bloom	179:183	bloom	179:183	Chronic inflammation and gut microbiota dysbiosis, in particular the bloom of genotoxin-producing E. coli strains, are risk factors for the development of colorectal cancer.
31358565	3	29	from	model	599:603	arg1	Expansion					493:501	Expansion	493:501	Expansion of experimentally introduced E. coli strains in the azoxymethane/dextran sulfate sodium colitis model	493:603	Expansion of experimentally introduced E. coli strains in the azoxymethane/dextran sulfate sodium colitis model was driven by molybdoenzyme-dependent metabolic pathways.
31358565	1	30	theme	genotoxin-producing	188:206	arg1	strains					216:222	genotoxin-producing E. coli strains	188:222	genotoxin-producing E. coli strains	188:222	Chronic inflammation and gut microbiota dysbiosis, in particular the bloom of genotoxin-producing E. coli strains, are risk factors for the development of colorectal cancer.
31358565	0	31	theme	gut	15:17	arg1	microbiota					19:28	the gut microbiota	11:28	the gut microbiota	11:28	Editing of the gut microbiota reduces carcinogenesis in mouse models of colitis-associated colorectal cancer.
31358565	3	32	theme	molybdoenzyme-dependent	619:641	arg1	pathways					653:660	molybdoenzyme-dependent metabolic pathways	619:660	molybdoenzyme-dependent metabolic pathways	619:660	Expansion of experimentally introduced E. coli strains in the azoxymethane/dextran sulfate sodium colitis model was driven by molybdoenzyme-dependent metabolic pathways.
31358565	3	33	from	Expansion	493:501	arg1	model					599:603	the azoxymethane/dextran sulfate sodium colitis model	551:603	the azoxymethane/dextran sulfate sodium colitis model	551:603	Expansion of experimentally introduced E. coli strains in the azoxymethane/dextran sulfate sodium colitis model was driven by molybdoenzyme-dependent metabolic pathways.
31358565	3	34	theme	azoxymethane/dextran	555:574	arg1	model					599:603	the azoxymethane/dextran sulfate sodium colitis model	551:603	the azoxymethane/dextran sulfate sodium colitis model	551:603	Expansion of experimentally introduced E. coli strains in the azoxymethane/dextran sulfate sodium colitis model was driven by molybdoenzyme-dependent metabolic pathways.
31358565	1	35	theme	E.	208:209	arg1	strains					216:222	genotoxin-producing E. coli strains	188:222	genotoxin-producing E. coli strains	188:222	Chronic inflammation and gut microbiota dysbiosis, in particular the bloom of genotoxin-producing E. coli strains, are risk factors for the development of colorectal cancer.
31358565	3	36	theme	colitis	591:597	arg1	model					599:603	the azoxymethane/dextran sulfate sodium colitis model	551:603	the azoxymethane/dextran sulfate sodium colitis model	551:603	Expansion of experimentally introduced E. coli strains in the azoxymethane/dextran sulfate sodium colitis model was driven by molybdoenzyme-dependent metabolic pathways.
31358565	5	37	theme	azoxymethane-treated	1044:1063	arg1	mice					1081:1084	azoxymethane-treated, Il10-deficient mice	1044:1084	azoxymethane-treated, Il10-deficient mice	1044:1084	Restricting the bloom of Enterobacteriaceae decreased intestinal inflammation and reduced the incidence of colonic tumors in two models of CAC, the azoxymethane/dextran sulfate sodium colitis model and azoxymethane-treated, Il10-deficient mice.
31358565	1	38	theme	Chronic	110:116	arg1	factors					234:240	risk factors	229:240	risk factors for the development of colorectal cancer	229:281	Chronic inflammation and gut microbiota dysbiosis, in particular the bloom of genotoxin-producing E. coli strains, are risk factors for the development of colorectal cancer.
31358565	1	38	theme	Chronic	110:116	arg1	inflammation					118:129	Chronic inflammation	110:129	Chronic inflammation	110:129	Chronic inflammation and gut microbiota dysbiosis, in particular the bloom of genotoxin-producing E. coli strains, are risk factors for the development of colorectal cancer.
31358565	1	38	theme	Chronic	110:116	arg1	dysbiosis					150:158	gut microbiota dysbiosis	135:158	gut microbiota dysbiosis	135:158	Chronic inflammation and gut microbiota dysbiosis, in particular the bloom of genotoxin-producing E. coli strains, are risk factors for the development of colorectal cancer.
31358565	2	39	theme	tumor	412:416	arg1	development					418:428	tumor development	412:428	tumor development in mouse models of colitis-associated colorectal cancer (CAC)	412:490	Here, we sought to determine whether precision editing of gut microbiota metabolism and composition could decrease the risk for tumor development in mouse models of colitis-associated colorectal cancer (CAC).
31358565	4	40	with	Enterobacteriaceae	822:839	arg1	coli					807:810	genotoxin-producing E. coli	784:810	genotoxin-producing E. coli	784:810	Oral administration of sodium tungstate inhibited E. coli molybdoenzymes and selectively decreased gut colonization with genotoxin-producing E. coli and other Enterobacteriaceae.
31358565	6	41	theme	gut	1264:1266	arg1	dysbiosis					1279:1287	gut microbiota dysbiosis	1264:1287	gut microbiota dysbiosis	1264:1287	We conclude that metabolic targeting of protumoral Enterobacteriaceae during chronic inflammation is a suitable strategy to prevent the development of malignancies arising from gut microbiota dysbiosis.
31358565	2	42	theme	composition	372:382	arg1	editing					331:337	precision editing	321:337	precision editing of gut microbiota metabolism and composition	321:382	Here, we sought to determine whether precision editing of gut microbiota metabolism and composition could decrease the risk for tumor development in mouse models of colitis-associated colorectal cancer (CAC).
31358565	4	43	theme	sodium	686:691	arg1	tungstate					693:701	sodium tungstate	686:701	sodium tungstate	686:701	Oral administration of sodium tungstate inhibited E. coli molybdoenzymes and selectively decreased gut colonization with genotoxin-producing E. coli and other Enterobacteriaceae.
31358565	0	44	theme	microbiota	19:28	arg1	Editing					0:6	Editing	0:6	Editing of the gut microbiota	0:28	Editing of the gut microbiota reduces carcinogenesis in mouse models of colitis-associated colorectal cancer.
31358565	2	45	theme	cancer	479:484	arg1	models					439:444	mouse models	433:444	mouse models of colitis-associated colorectal cancer (CAC)	433:490	Here, we sought to determine whether precision editing of gut microbiota metabolism and composition could decrease the risk for tumor development in mouse models of colitis-associated colorectal cancer (CAC).
31358565	2	46	theme	mouse	433:437	arg1	models					439:444	mouse models	433:444	mouse models of colitis-associated colorectal cancer (CAC)	433:490	Here, we sought to determine whether precision editing of gut microbiota metabolism and composition could decrease the risk for tumor development in mouse models of colitis-associated colorectal cancer (CAC).
31358565	5	47	theme	colonic	949:955	arg1	tumors					957:962	colonic tumors	949:962	colonic tumors	949:962	Restricting the bloom of Enterobacteriaceae decreased intestinal inflammation and reduced the incidence of colonic tumors in two models of CAC, the azoxymethane/dextran sulfate sodium colitis model and azoxymethane-treated, Il10-deficient mice.
31358565	2	48	theme	colorectal	468:477	arg1	CAC					487:489	CAC	487:489	CAC	487:489	Here, we sought to determine whether precision editing of gut microbiota metabolism and composition could decrease the risk for tumor development in mouse models of colitis-associated colorectal cancer (CAC).
31358565	2	48	theme	colorectal	468:477	arg1	cancer					479:484	colitis-associated colorectal cancer	449:484	colitis-associated colorectal cancer (CAC)	449:490	Here, we sought to determine whether precision editing of gut microbiota metabolism and composition could decrease the risk for tumor development in mouse models of colitis-associated colorectal cancer (CAC).
31358565	6	49	theme	malignancies	1238:1249	arg1	development					1223:1233	the development	1219:1233	the development of malignancies arising from gut microbiota dysbiosis	1219:1287	We conclude that metabolic targeting of protumoral Enterobacteriaceae during chronic inflammation is a suitable strategy to prevent the development of malignancies arising from gut microbiota dysbiosis.
31358565	5	50	theme	azoxymethane/dextran	990:1009	arg1	model					1034:1038	the azoxymethane/dextran sulfate sodium colitis model	986:1038	the azoxymethane/dextran sulfate sodium colitis model	986:1038	Restricting the bloom of Enterobacteriaceae decreased intestinal inflammation and reduced the incidence of colonic tumors in two models of CAC, the azoxymethane/dextran sulfate sodium colitis model and azoxymethane-treated, Il10-deficient mice.
31358565	2	51	theme	colitis-associated	449:466	arg1	CAC					487:489	CAC	487:489	CAC	487:489	Here, we sought to determine whether precision editing of gut microbiota metabolism and composition could decrease the risk for tumor development in mouse models of colitis-associated colorectal cancer (CAC).
31358565	2	51	theme	colitis-associated	449:466	arg1	cancer					479:484	colitis-associated colorectal cancer	449:484	colitis-associated colorectal cancer (CAC)	449:490	Here, we sought to determine whether precision editing of gut microbiota metabolism and composition could decrease the risk for tumor development in mouse models of colitis-associated colorectal cancer (CAC).
31358565	6	52	theme	suitable	1190:1197	arg1	strategy					1199:1206	a suitable strategy	1188:1206	a suitable strategy to prevent the development of malignancies arising from gut microbiota dysbiosis	1188:1287	We conclude that metabolic targeting of protumoral Enterobacteriaceae during chronic inflammation is a suitable strategy to prevent the development of malignancies arising from gut microbiota dysbiosis.
31358565	6	52	theme	suitable	1190:1197	arg1	targeting					1114:1122	metabolic targeting	1104:1122	metabolic targeting of protumoral Enterobacteriaceae during chronic inflammation	1104:1183	We conclude that metabolic targeting of protumoral Enterobacteriaceae during chronic inflammation is a suitable strategy to prevent the development of malignancies arising from gut microbiota dysbiosis.
31358565	4	53	theme	gut	762:764	arg1	colonization					766:777	gut colonization	762:777	gut colonization with genotoxin-producing E. coli	762:810	Oral administration of sodium tungstate inhibited E. coli molybdoenzymes and selectively decreased gut colonization with genotoxin-producing E. coli and other Enterobacteriaceae.
31358565	4	54	theme	E.	713:714	arg1	molybdoenzymes					721:734	E. coli molybdoenzymes	713:734	E. coli molybdoenzymes	713:734	Oral administration of sodium tungstate inhibited E. coli molybdoenzymes and selectively decreased gut colonization with genotoxin-producing E. coli and other Enterobacteriaceae.
31358565	5	55	from	incidence	936:944	arg1	mice					1081:1084	azoxymethane-treated, Il10-deficient mice	1044:1084	azoxymethane-treated, Il10-deficient mice	1044:1084	Restricting the bloom of Enterobacteriaceae decreased intestinal inflammation and reduced the incidence of colonic tumors in two models of CAC, the azoxymethane/dextran sulfate sodium colitis model and azoxymethane-treated, Il10-deficient mice.
31358565	5	55	from	incidence	936:944	arg1	model					1034:1038	the azoxymethane/dextran sulfate sodium colitis model	986:1038	the azoxymethane/dextran sulfate sodium colitis model	986:1038	Restricting the bloom of Enterobacteriaceae decreased intestinal inflammation and reduced the incidence of colonic tumors in two models of CAC, the azoxymethane/dextran sulfate sodium colitis model and azoxymethane-treated, Il10-deficient mice.
31358565	5	55	from	incidence	936:944	arg1	models					971:976	two models	967:976	two models of CAC	967:983	Restricting the bloom of Enterobacteriaceae decreased intestinal inflammation and reduced the incidence of colonic tumors in two models of CAC, the azoxymethane/dextran sulfate sodium colitis model and azoxymethane-treated, Il10-deficient mice.
31358565	1	56	theme	gut	135:137	arg1	factors					234:240	risk factors	229:240	risk factors for the development of colorectal cancer	229:281	Chronic inflammation and gut microbiota dysbiosis, in particular the bloom of genotoxin-producing E. coli strains, are risk factors for the development of colorectal cancer.
31358565	1	56	theme	gut	135:137	arg1	inflammation					118:129	Chronic inflammation	110:129	Chronic inflammation	110:129	Chronic inflammation and gut microbiota dysbiosis, in particular the bloom of genotoxin-producing E. coli strains, are risk factors for the development of colorectal cancer.
31358565	1	56	theme	gut	135:137	arg1	dysbiosis					150:158	gut microbiota dysbiosis	135:158	gut microbiota dysbiosis	135:158	Chronic inflammation and gut microbiota dysbiosis, in particular the bloom of genotoxin-producing E. coli strains, are risk factors for the development of colorectal cancer.
31358565	4	57	theme	other	816:820	arg1	Enterobacteriaceae					822:839	other Enterobacteriaceae	816:839	other Enterobacteriaceae	816:839	Oral administration of sodium tungstate inhibited E. coli molybdoenzymes and selectively decreased gut colonization with genotoxin-producing E. coli and other Enterobacteriaceae.
31358565	3	58	theme	strains	540:546	arg1	Expansion					493:501	Expansion	493:501	Expansion of experimentally introduced E. coli strains in the azoxymethane/dextran sulfate sodium colitis model	493:603	Expansion of experimentally introduced E. coli strains in the azoxymethane/dextran sulfate sodium colitis model was driven by molybdoenzyme-dependent metabolic pathways.
31358565	4	59	dep	E.	713:714	arg1	coli					716:719	coli	716:719	coli	716:719	Oral administration of sodium tungstate inhibited E. coli molybdoenzymes and selectively decreased gut colonization with genotoxin-producing E. coli and other Enterobacteriaceae.
31358565	1	60	theme	microbiota	139:148	arg1	factors					234:240	risk factors	229:240	risk factors for the development of colorectal cancer	229:281	Chronic inflammation and gut microbiota dysbiosis, in particular the bloom of genotoxin-producing E. coli strains, are risk factors for the development of colorectal cancer.
31358565	1	60	theme	microbiota	139:148	arg1	inflammation					118:129	Chronic inflammation	110:129	Chronic inflammation	110:129	Chronic inflammation and gut microbiota dysbiosis, in particular the bloom of genotoxin-producing E. coli strains, are risk factors for the development of colorectal cancer.
31358565	1	60	theme	microbiota	139:148	arg1	dysbiosis					150:158	gut microbiota dysbiosis	135:158	gut microbiota dysbiosis	135:158	Chronic inflammation and gut microbiota dysbiosis, in particular the bloom of genotoxin-producing E. coli strains, are risk factors for the development of colorectal cancer.
31358565	2	61	theme	precision	321:329	arg1	editing					331:337	precision editing	321:337	precision editing of gut microbiota metabolism and composition	321:382	Here, we sought to determine whether precision editing of gut microbiota metabolism and composition could decrease the risk for tumor development in mouse models of colitis-associated colorectal cancer (CAC).
31358565	6	62	theme	chronic	1164:1170	arg1	inflammation					1172:1183	chronic inflammation	1164:1183	chronic inflammation	1164:1183	We conclude that metabolic targeting of protumoral Enterobacteriaceae during chronic inflammation is a suitable strategy to prevent the development of malignancies arising from gut microbiota dysbiosis.
31358565	1	63	dep	E.	208:209	arg1	coli					211:214	coli	211:214	coli	211:214	Chronic inflammation and gut microbiota dysbiosis, in particular the bloom of genotoxin-producing E. coli strains, are risk factors for the development of colorectal cancer.
31358565	3	64	theme	introduced	521:530	arg1	strains					540:546	experimentally introduced E. coli strains	506:546	experimentally introduced E. coli strains in the azoxymethane/dextran sulfate sodium colitis model	506:603	Expansion of experimentally introduced E. coli strains in the azoxymethane/dextran sulfate sodium colitis model was driven by molybdoenzyme-dependent metabolic pathways.
31358565	6	65	theme	protumoral	1127:1136	arg1	Enterobacteriaceae					1138:1155	protumoral Enterobacteriaceae	1127:1155	protumoral Enterobacteriaceae	1127:1155	We conclude that metabolic targeting of protumoral Enterobacteriaceae during chronic inflammation is a suitable strategy to prevent the development of malignancies arising from gut microbiota dysbiosis.
31358565	0	66	theme	mouse	56:60	arg1	models					62:67	mouse models	56:67	mouse models of colitis-associated colorectal cancer	56:107	Editing of the gut microbiota reduces carcinogenesis in mouse models of colitis-associated colorectal cancer.
31358565	3	67	theme	metabolic	643:651	arg1	pathways					653:660	molybdoenzyme-dependent metabolic pathways	619:660	molybdoenzyme-dependent metabolic pathways	619:660	Expansion of experimentally introduced E. coli strains in the azoxymethane/dextran sulfate sodium colitis model was driven by molybdoenzyme-dependent metabolic pathways.
31358565	2	68	from	development	418:428	arg1	models					439:444	mouse models	433:444	mouse models of colitis-associated colorectal cancer (CAC)	433:490	Here, we sought to determine whether precision editing of gut microbiota metabolism and composition could decrease the risk for tumor development in mouse models of colitis-associated colorectal cancer (CAC).
31358565	2	69	theme	metabolism	357:366	arg1	editing					331:337	precision editing	321:337	precision editing of gut microbiota metabolism and composition	321:382	Here, we sought to determine whether precision editing of gut microbiota metabolism and composition could decrease the risk for tumor development in mouse models of colitis-associated colorectal cancer (CAC).
31446590	1	0	from	medicine	194:201	arg1	China					215:219	southern China	206:219	southern China	206:219	Rhynchosia minima root, a folk herbal medicine in southern China, is used to relieve itch and swelling.
31446590	3	1	theme	compound	462:469	arg1	composition					471:481	The compound composition	458:481	The compound composition of EEF6	458:489	The compound composition of EEF6 was determined by high-performance liquid chromatography-mass spectrometry.
31446590	0	2	theme	RAW	106:108	arg1	cells					116:120	lipopolysaccharide-stimulated RAW 264.7 cells	76:120	lipopolysaccharide-stimulated RAW 264.7 cells	76:120	Flavonoids from Rhynchosia minima root exerts anti-inflammatory activity in lipopolysaccharide-stimulated RAW 264.7 cells via MAPK/NF-κB signaling pathway.
31446590	1	3	theme	folk	182:185	arg1	medicine					194:201	a folk herbal medicine	180:201	a folk herbal medicine in southern China	180:219	Rhynchosia minima root, a folk herbal medicine in southern China, is used to relieve itch and swelling.
31446590	1	3	theme	folk	182:185	arg1	root					174:177	Rhynchosia minima root	156:177	Rhynchosia minima root	156:177	Rhynchosia minima root, a folk herbal medicine in southern China, is used to relieve itch and swelling.
31446590	5	4	from	cytoplasm	1034:1042	arg1	translocation					1008:1020	the translocation	1004:1020	the translocation of p65 from cytoplasm to nucleus	1004:1053	In addition, EEF6 exhibited potent anti-inflammatory ability against LPS-stimulated RAW 264.7 cells via MAPK/NF-κB signaling pathways by decreasing the secretion of nitric oxide (NO), interleukin (IL)-6, TNF-α, and monocyte chemotactic protein (MCP)-1, inhibiting the translocation of p65 from cytoplasm to nucleus, and suppressing the phosphorylation of ERK, JNK, and p38.
31446590	2	5	from	property	309:316	arg1	cells					414:418	lipopolysaccharide (LPS)-induced RAW 264.7 cells	371:418	lipopolysaccharide (LPS)-induced RAW 264.7 cells	371:418	In this study, we examined the anti-inflammatory property of an ethanol fraction (EEF6) from R. minima root on lipopolysaccharide (LPS)-induced RAW 264.7 cells, as well as its underlying mechanism.
31446590	2	5	from	property	309:316	arg1	root					363:366	R. minima root	353:366	R. minima root	353:366	In this study, we examined the anti-inflammatory property of an ethanol fraction (EEF6) from R. minima root on lipopolysaccharide (LPS)-induced RAW 264.7 cells, as well as its underlying mechanism.
31446590	2	5	from	property	309:316	arg1	mechanism					447:455	its underlying mechanism	432:455	its underlying mechanism	432:455	In this study, we examined the anti-inflammatory property of an ethanol fraction (EEF6) from R. minima root on lipopolysaccharide (LPS)-induced RAW 264.7 cells, as well as its underlying mechanism.
31446590	0	6	from	activity	64:71	arg1	cells					116:120	lipopolysaccharide-stimulated RAW 264.7 cells	76:120	lipopolysaccharide-stimulated RAW 264.7 cells	76:120	Flavonoids from Rhynchosia minima root exerts anti-inflammatory activity in lipopolysaccharide-stimulated RAW 264.7 cells via MAPK/NF-κB signaling pathway.
31446590	6	7	theme	inflammation	1184:1195	arg1	management					1197:1206	inflammation management	1184:1206	inflammation management	1184:1206	These results indicated that EEF6 could be a promising ingredient for inflammation management.
31446590	3	8	theme	high-performance	509:524	arg1	spectrometry					553:564	high-performance liquid chromatography-mass spectrometry	509:564	high-performance liquid chromatography-mass spectrometry	509:564	The compound composition of EEF6 was determined by high-performance liquid chromatography-mass spectrometry.
31446590	0	9	theme	signaling	137:145	arg1	pathway					147:153	MAPK/NF-κB signaling pathway	126:153	MAPK/NF-κB signaling pathway	126:153	Flavonoids from Rhynchosia minima root exerts anti-inflammatory activity in lipopolysaccharide-stimulated RAW 264.7 cells via MAPK/NF-κB signaling pathway.
31446590	1	10	theme	herbal	187:192	arg1	medicine					194:201	a folk herbal medicine	180:201	a folk herbal medicine in southern China	180:219	Rhynchosia minima root, a folk herbal medicine in southern China, is used to relieve itch and swelling.
31446590	1	10	theme	herbal	187:192	arg1	root					174:177	Rhynchosia minima root	156:177	Rhynchosia minima root	156:177	Rhynchosia minima root, a folk herbal medicine in southern China, is used to relieve itch and swelling.
31446590	0	11	theme	MAPK/NF-κB	126:135	arg1	pathway					147:153	MAPK/NF-κB signaling pathway	126:153	MAPK/NF-κB signaling pathway	126:153	Flavonoids from Rhynchosia minima root exerts anti-inflammatory activity in lipopolysaccharide-stimulated RAW 264.7 cells via MAPK/NF-κB signaling pathway.
31446590	2	12	theme	underlying	436:445	arg1	mechanism					447:455	its underlying mechanism	432:455	its underlying mechanism	432:455	In this study, we examined the anti-inflammatory property of an ethanol fraction (EEF6) from R. minima root on lipopolysaccharide (LPS)-induced RAW 264.7 cells, as well as its underlying mechanism.
31446590	5	13	theme	signaling	855:863	arg1	pathways					865:872	MAPK/NF-κB signaling pathways	844:872	MAPK/NF-κB signaling pathways	844:872	In addition, EEF6 exhibited potent anti-inflammatory ability against LPS-stimulated RAW 264.7 cells via MAPK/NF-κB signaling pathways by decreasing the secretion of nitric oxide (NO), interleukin (IL)-6, TNF-α, and monocyte chemotactic protein (MCP)-1, inhibiting the translocation of p65 from cytoplasm to nucleus, and suppressing the phosphorylation of ERK, JNK, and p38.
31446590	5	14	theme	anti-inflammatory	775:791	arg1	ability					793:799	potent anti-inflammatory ability	768:799	potent anti-inflammatory ability	768:799	In addition, EEF6 exhibited potent anti-inflammatory ability against LPS-stimulated RAW 264.7 cells via MAPK/NF-κB signaling pathways by decreasing the secretion of nitric oxide (NO), interleukin (IL)-6, TNF-α, and monocyte chemotactic protein (MCP)-1, inhibiting the translocation of p65 from cytoplasm to nucleus, and suppressing the phosphorylation of ERK, JNK, and p38.
31446590	0	15	from	root	34:37	arg1	Flavonoids					0:9	Flavonoids	0:9	Flavonoids from Rhynchosia minima root	0:37	Flavonoids from Rhynchosia minima root exerts anti-inflammatory activity in lipopolysaccharide-stimulated RAW 264.7 cells via MAPK/NF-κB signaling pathway.
31446590	6	16	theme	promising	1159:1167	arg1	ingredient					1169:1178	a promising ingredient	1157:1178	a promising ingredient for inflammation management	1157:1206	These results indicated that EEF6 could be a promising ingredient for inflammation management.
31446590	6	16	theme	promising	1159:1167	arg1	EEF6					1143:1146	EEF6	1143:1146	EEF6	1143:1146	These results indicated that EEF6 could be a promising ingredient for inflammation management.
31446590	5	17	theme	interleukin	924:934	arg1	secretion					892:900	the secretion	888:900	the secretion of nitric oxide (NO), interleukin (IL)-6, TNF-α, and monocyte chemotactic protein (MCP)-1	888:990	In addition, EEF6 exhibited potent anti-inflammatory ability against LPS-stimulated RAW 264.7 cells via MAPK/NF-κB signaling pathways by decreasing the secretion of nitric oxide (NO), interleukin (IL)-6, TNF-α, and monocyte chemotactic protein (MCP)-1, inhibiting the translocation of p65 from cytoplasm to nucleus, and suppressing the phosphorylation of ERK, JNK, and p38.
31446590	5	18	theme	JNK	1100:1102	arg1	phosphorylation					1076:1090	the phosphorylation	1072:1090	the phosphorylation of ERK, JNK, and p38	1072:1111	In addition, EEF6 exhibited potent anti-inflammatory ability against LPS-stimulated RAW 264.7 cells via MAPK/NF-κB signaling pathways by decreasing the secretion of nitric oxide (NO), interleukin (IL)-6, TNF-α, and monocyte chemotactic protein (MCP)-1, inhibiting the translocation of p65 from cytoplasm to nucleus, and suppressing the phosphorylation of ERK, JNK, and p38.
31446590	2	19	theme	minima	356:361	arg1	root					363:366	R. minima root	353:366	R. minima root	353:366	In this study, we examined the anti-inflammatory property of an ethanol fraction (EEF6) from R. minima root on lipopolysaccharide (LPS)-induced RAW 264.7 cells, as well as its underlying mechanism.
31446590	0	20	theme	minima	27:32	arg1	root					34:37	Rhynchosia minima root	16:37	Rhynchosia minima root	16:37	Flavonoids from Rhynchosia minima root exerts anti-inflammatory activity in lipopolysaccharide-stimulated RAW 264.7 cells via MAPK/NF-κB signaling pathway.
31446590	2	21	theme	anti-inflammatory	291:307	arg1	property					309:316	the anti-inflammatory property	287:316	the anti-inflammatory property of an ethanol fraction (EEF6) from R. minima root on lipopolysaccharide (LPS)-induced RAW 264.7 cells, as well as its underlying mechanism	287:455	In this study, we examined the anti-inflammatory property of an ethanol fraction (EEF6) from R. minima root on lipopolysaccharide (LPS)-induced RAW 264.7 cells, as well as its underlying mechanism.
31446590	5	22	theme	chemotactic	964:974	arg1	MCP					985:987	monocyte chemotactic protein (MCP)-1	955:990	monocyte chemotactic protein (MCP)-1	955:990	In addition, EEF6 exhibited potent anti-inflammatory ability against LPS-stimulated RAW 264.7 cells via MAPK/NF-κB signaling pathways by decreasing the secretion of nitric oxide (NO), interleukin (IL)-6, TNF-α, and monocyte chemotactic protein (MCP)-1, inhibiting the translocation of p65 from cytoplasm to nucleus, and suppressing the phosphorylation of ERK, JNK, and p38.
31446590	2	23	theme	-induced	395:402	arg1	cells					414:418	lipopolysaccharide (LPS)-induced RAW 264.7 cells	371:418	lipopolysaccharide (LPS)-induced RAW 264.7 cells	371:418	In this study, we examined the anti-inflammatory property of an ethanol fraction (EEF6) from R. minima root on lipopolysaccharide (LPS)-induced RAW 264.7 cells, as well as its underlying mechanism.
31446590	2	24	theme	RAW	404:406	arg1	cells					414:418	lipopolysaccharide (LPS)-induced RAW 264.7 cells	371:418	lipopolysaccharide (LPS)-induced RAW 264.7 cells	371:418	In this study, we examined the anti-inflammatory property of an ethanol fraction (EEF6) from R. minima root on lipopolysaccharide (LPS)-induced RAW 264.7 cells, as well as its underlying mechanism.
31446590	3	25	theme	chromatography-mass	533:551	arg1	spectrometry					553:564	high-performance liquid chromatography-mass spectrometry	509:564	high-performance liquid chromatography-mass spectrometry	509:564	The compound composition of EEF6 was determined by high-performance liquid chromatography-mass spectrometry.
31446590	5	26	theme	p38	1109:1111	arg1	phosphorylation					1076:1090	the phosphorylation	1072:1090	the phosphorylation of ERK, JNK, and p38	1072:1111	In addition, EEF6 exhibited potent anti-inflammatory ability against LPS-stimulated RAW 264.7 cells via MAPK/NF-κB signaling pathways by decreasing the secretion of nitric oxide (NO), interleukin (IL)-6, TNF-α, and monocyte chemotactic protein (MCP)-1, inhibiting the translocation of p65 from cytoplasm to nucleus, and suppressing the phosphorylation of ERK, JNK, and p38.
31446590	1	27	used	used	225:228	arg2	root					174:177	Rhynchosia minima root	156:177	Rhynchosia minima root	156:177	Rhynchosia minima root, a folk herbal medicine in southern China, is used to relieve itch and swelling.
31446590	1	27	used	used	225:228	arg2	medicine					194:201	a folk herbal medicine	180:201	a folk herbal medicine in southern China	180:219	Rhynchosia minima root, a folk herbal medicine in southern China, is used to relieve itch and swelling.
31446590	1	28	theme	southern	206:213	arg1	China					215:219	southern China	206:219	southern China	206:219	Rhynchosia minima root, a folk herbal medicine in southern China, is used to relieve itch and swelling.
31446590	5	29	theme	MAPK/NF-κB	844:853	arg1	pathways					865:872	MAPK/NF-κB signaling pathways	844:872	MAPK/NF-κB signaling pathways	844:872	In addition, EEF6 exhibited potent anti-inflammatory ability against LPS-stimulated RAW 264.7 cells via MAPK/NF-κB signaling pathways by decreasing the secretion of nitric oxide (NO), interleukin (IL)-6, TNF-α, and monocyte chemotactic protein (MCP)-1, inhibiting the translocation of p65 from cytoplasm to nucleus, and suppressing the phosphorylation of ERK, JNK, and p38.
31446590	5	30	theme	protein	976:982	arg1	MCP					985:987	monocyte chemotactic protein (MCP)-1	955:990	monocyte chemotactic protein (MCP)-1	955:990	In addition, EEF6 exhibited potent anti-inflammatory ability against LPS-stimulated RAW 264.7 cells via MAPK/NF-κB signaling pathways by decreasing the secretion of nitric oxide (NO), interleukin (IL)-6, TNF-α, and monocyte chemotactic protein (MCP)-1, inhibiting the translocation of p65 from cytoplasm to nucleus, and suppressing the phosphorylation of ERK, JNK, and p38.
31446590	2	31	from	root	363:366	arg1	fraction					332:339	an ethanol fraction	321:339	an ethanol fraction (EEF6) from R. minima root	321:366	In this study, we examined the anti-inflammatory property of an ethanol fraction (EEF6) from R. minima root on lipopolysaccharide (LPS)-induced RAW 264.7 cells, as well as its underlying mechanism.
31446590	2	31	from	root	363:366	arg1	property					309:316	the anti-inflammatory property	287:316	the anti-inflammatory property of an ethanol fraction (EEF6) from R. minima root on lipopolysaccharide (LPS)-induced RAW 264.7 cells, as well as its underlying mechanism	287:455	In this study, we examined the anti-inflammatory property of an ethanol fraction (EEF6) from R. minima root on lipopolysaccharide (LPS)-induced RAW 264.7 cells, as well as its underlying mechanism.
31446590	2	31	from	root	363:366	arg1	EEF6					342:345	EEF6	342:345	EEF6	342:345	In this study, we examined the anti-inflammatory property of an ethanol fraction (EEF6) from R. minima root on lipopolysaccharide (LPS)-induced RAW 264.7 cells, as well as its underlying mechanism.
31446590	5	32	theme	potent	768:773	arg1	ability					793:799	potent anti-inflammatory ability	768:799	potent anti-inflammatory ability	768:799	In addition, EEF6 exhibited potent anti-inflammatory ability against LPS-stimulated RAW 264.7 cells via MAPK/NF-κB signaling pathways by decreasing the secretion of nitric oxide (NO), interleukin (IL)-6, TNF-α, and monocyte chemotactic protein (MCP)-1, inhibiting the translocation of p65 from cytoplasm to nucleus, and suppressing the phosphorylation of ERK, JNK, and p38.
31446590	5	33	theme	p65	1025:1027	arg1	translocation					1008:1020	the translocation	1004:1020	the translocation of p65 from cytoplasm to nucleus	1004:1053	In addition, EEF6 exhibited potent anti-inflammatory ability against LPS-stimulated RAW 264.7 cells via MAPK/NF-κB signaling pathways by decreasing the secretion of nitric oxide (NO), interleukin (IL)-6, TNF-α, and monocyte chemotactic protein (MCP)-1, inhibiting the translocation of p65 from cytoplasm to nucleus, and suppressing the phosphorylation of ERK, JNK, and p38.
31446590	5	34	theme	nitric	905:910	arg1	oxide					912:916	nitric oxide	905:916	nitric oxide (NO)	905:921	In addition, EEF6 exhibited potent anti-inflammatory ability against LPS-stimulated RAW 264.7 cells via MAPK/NF-κB signaling pathways by decreasing the secretion of nitric oxide (NO), interleukin (IL)-6, TNF-α, and monocyte chemotactic protein (MCP)-1, inhibiting the translocation of p65 from cytoplasm to nucleus, and suppressing the phosphorylation of ERK, JNK, and p38.
31446590	5	34	theme	nitric	905:910	arg1	NO					919:920	NO	919:920	NO	919:920	In addition, EEF6 exhibited potent anti-inflammatory ability against LPS-stimulated RAW 264.7 cells via MAPK/NF-κB signaling pathways by decreasing the secretion of nitric oxide (NO), interleukin (IL)-6, TNF-α, and monocyte chemotactic protein (MCP)-1, inhibiting the translocation of p65 from cytoplasm to nucleus, and suppressing the phosphorylation of ERK, JNK, and p38.
31446590	0	35	theme	anti-inflammatory	46:62	arg1	activity					64:71	anti-inflammatory activity	46:71	anti-inflammatory activity in lipopolysaccharide-stimulated RAW 264.7 cells	46:120	Flavonoids from Rhynchosia minima root exerts anti-inflammatory activity in lipopolysaccharide-stimulated RAW 264.7 cells via MAPK/NF-κB signaling pathway.
31446590	5	36	theme	ERK	1095:1097	arg1	phosphorylation					1076:1090	the phosphorylation	1072:1090	the phosphorylation of ERK, JNK, and p38	1072:1111	In addition, EEF6 exhibited potent anti-inflammatory ability against LPS-stimulated RAW 264.7 cells via MAPK/NF-κB signaling pathways by decreasing the secretion of nitric oxide (NO), interleukin (IL)-6, TNF-α, and monocyte chemotactic protein (MCP)-1, inhibiting the translocation of p65 from cytoplasm to nucleus, and suppressing the phosphorylation of ERK, JNK, and p38.
31446590	5	37	theme	MCP	985:987	arg1	secretion					892:900	the secretion	888:900	the secretion of nitric oxide (NO), interleukin (IL)-6, TNF-α, and monocyte chemotactic protein (MCP)-1	888:990	In addition, EEF6 exhibited potent anti-inflammatory ability against LPS-stimulated RAW 264.7 cells via MAPK/NF-κB signaling pathways by decreasing the secretion of nitric oxide (NO), interleukin (IL)-6, TNF-α, and monocyte chemotactic protein (MCP)-1, inhibiting the translocation of p65 from cytoplasm to nucleus, and suppressing the phosphorylation of ERK, JNK, and p38.
31446590	2	38	theme	fraction	332:339	arg1	property					309:316	the anti-inflammatory property	287:316	the anti-inflammatory property of an ethanol fraction (EEF6) from R. minima root on lipopolysaccharide (LPS)-induced RAW 264.7 cells, as well as its underlying mechanism	287:455	In this study, we examined the anti-inflammatory property of an ethanol fraction (EEF6) from R. minima root on lipopolysaccharide (LPS)-induced RAW 264.7 cells, as well as its underlying mechanism.
31446590	5	39	theme	monocyte	955:962	arg1	MCP					985:987	monocyte chemotactic protein (MCP)-1	955:990	monocyte chemotactic protein (MCP)-1	955:990	In addition, EEF6 exhibited potent anti-inflammatory ability against LPS-stimulated RAW 264.7 cells via MAPK/NF-κB signaling pathways by decreasing the secretion of nitric oxide (NO), interleukin (IL)-6, TNF-α, and monocyte chemotactic protein (MCP)-1, inhibiting the translocation of p65 from cytoplasm to nucleus, and suppressing the phosphorylation of ERK, JNK, and p38.
31446590	5	40	theme	oxide	912:916	arg1	secretion					892:900	the secretion	888:900	the secretion of nitric oxide (NO), interleukin (IL)-6, TNF-α, and monocyte chemotactic protein (MCP)-1	888:990	In addition, EEF6 exhibited potent anti-inflammatory ability against LPS-stimulated RAW 264.7 cells via MAPK/NF-κB signaling pathways by decreasing the secretion of nitric oxide (NO), interleukin (IL)-6, TNF-α, and monocyte chemotactic protein (MCP)-1, inhibiting the translocation of p65 from cytoplasm to nucleus, and suppressing the phosphorylation of ERK, JNK, and p38.
31446590	5	41	theme	TNF-α	944:948	arg1	secretion					892:900	the secretion	888:900	the secretion of nitric oxide (NO), interleukin (IL)-6, TNF-α, and monocyte chemotactic protein (MCP)-1	888:990	In addition, EEF6 exhibited potent anti-inflammatory ability against LPS-stimulated RAW 264.7 cells via MAPK/NF-κB signaling pathways by decreasing the secretion of nitric oxide (NO), interleukin (IL)-6, TNF-α, and monocyte chemotactic protein (MCP)-1, inhibiting the translocation of p65 from cytoplasm to nucleus, and suppressing the phosphorylation of ERK, JNK, and p38.
31446590	1	42	theme	Rhynchosia	156:165	arg1	medicine					194:201	a folk herbal medicine	180:201	a folk herbal medicine in southern China	180:219	Rhynchosia minima root, a folk herbal medicine in southern China, is used to relieve itch and swelling.
31446590	1	42	theme	Rhynchosia	156:165	arg1	root					174:177	Rhynchosia minima root	156:177	Rhynchosia minima root	156:177	Rhynchosia minima root, a folk herbal medicine in southern China, is used to relieve itch and swelling.
31446590	3	43	theme	liquid	526:531	arg1	spectrometry					553:564	high-performance liquid chromatography-mass spectrometry	509:564	high-performance liquid chromatography-mass spectrometry	509:564	The compound composition of EEF6 was determined by high-performance liquid chromatography-mass spectrometry.
31446590	4	44	theme	flavonoids	595:604	arg1	2					617:617	2	617:617	2	617:617	The result showed that five flavonoids compounds, 2',4',5,7-tetrahydroxyisoflavone, genistein-8-C-glucopyranoside, tricin, genistein, and daidzein, were identified in EEF6.
31446590	4	44	theme	flavonoids	595:604	arg1	daidzein					705:712	daidzein	705:712	daidzein	705:712	The result showed that five flavonoids compounds, 2',4',5,7-tetrahydroxyisoflavone, genistein-8-C-glucopyranoside, tricin, genistein, and daidzein, were identified in EEF6.
31446590	4	44	theme	flavonoids	595:604	arg1	genistein-8-C-glucopyranoside					651:679	genistein-8-C-glucopyranoside	651:679	genistein-8-C-glucopyranoside	651:679	The result showed that five flavonoids compounds, 2',4',5,7-tetrahydroxyisoflavone, genistein-8-C-glucopyranoside, tricin, genistein, and daidzein, were identified in EEF6.
31446590	4	44	theme	flavonoids	595:604	arg1	4					620:620	4	620:620	4	620:620	The result showed that five flavonoids compounds, 2',4',5,7-tetrahydroxyisoflavone, genistein-8-C-glucopyranoside, tricin, genistein, and daidzein, were identified in EEF6.
31446590	4	44	theme	flavonoids	595:604	arg1	tricin					682:687	tricin	682:687	tricin	682:687	The result showed that five flavonoids compounds, 2',4',5,7-tetrahydroxyisoflavone, genistein-8-C-glucopyranoside, tricin, genistein, and daidzein, were identified in EEF6.
31446590	4	44	theme	flavonoids	595:604	arg1	compounds					606:614	five flavonoids compounds	590:614	five flavonoids compounds	590:614	The result showed that five flavonoids compounds, 2',4',5,7-tetrahydroxyisoflavone, genistein-8-C-glucopyranoside, tricin, genistein, and daidzein, were identified in EEF6.
31446590	4	44	theme	flavonoids	595:604	arg1	5,7-tetrahydroxyisoflavone					623:648	5,7-tetrahydroxyisoflavone	623:648	5,7-tetrahydroxyisoflavone	623:648	The result showed that five flavonoids compounds, 2',4',5,7-tetrahydroxyisoflavone, genistein-8-C-glucopyranoside, tricin, genistein, and daidzein, were identified in EEF6.
31446590	4	44	theme	flavonoids	595:604	arg1	genistein					690:698	genistein	690:698	genistein	690:698	The result showed that five flavonoids compounds, 2',4',5,7-tetrahydroxyisoflavone, genistein-8-C-glucopyranoside, tricin, genistein, and daidzein, were identified in EEF6.
31446590	5	45	theme	LPS-stimulated	809:822	arg1	cells					834:838	LPS-stimulated RAW 264.7 cells	809:838	LPS-stimulated RAW 264.7 cells	809:838	In addition, EEF6 exhibited potent anti-inflammatory ability against LPS-stimulated RAW 264.7 cells via MAPK/NF-κB signaling pathways by decreasing the secretion of nitric oxide (NO), interleukin (IL)-6, TNF-α, and monocyte chemotactic protein (MCP)-1, inhibiting the translocation of p65 from cytoplasm to nucleus, and suppressing the phosphorylation of ERK, JNK, and p38.
31446590	1	46	theme	minima	167:172	arg1	medicine					194:201	a folk herbal medicine	180:201	a folk herbal medicine in southern China	180:219	Rhynchosia minima root, a folk herbal medicine in southern China, is used to relieve itch and swelling.
31446590	1	46	theme	minima	167:172	arg1	root					174:177	Rhynchosia minima root	156:177	Rhynchosia minima root	156:177	Rhynchosia minima root, a folk herbal medicine in southern China, is used to relieve itch and swelling.
31446590	2	47	theme	ethanol	324:330	arg1	fraction					332:339	an ethanol fraction	321:339	an ethanol fraction (EEF6) from R. minima root	321:366	In this study, we examined the anti-inflammatory property of an ethanol fraction (EEF6) from R. minima root on lipopolysaccharide (LPS)-induced RAW 264.7 cells, as well as its underlying mechanism.
31446590	2	47	theme	ethanol	324:330	arg1	EEF6					342:345	EEF6	342:345	EEF6	342:345	In this study, we examined the anti-inflammatory property of an ethanol fraction (EEF6) from R. minima root on lipopolysaccharide (LPS)-induced RAW 264.7 cells, as well as its underlying mechanism.
31446590	0	48	theme	lipopolysaccharide-stimulated	76:104	arg1	cells					116:120	lipopolysaccharide-stimulated RAW 264.7 cells	76:120	lipopolysaccharide-stimulated RAW 264.7 cells	76:120	Flavonoids from Rhynchosia minima root exerts anti-inflammatory activity in lipopolysaccharide-stimulated RAW 264.7 cells via MAPK/NF-κB signaling pathway.
31446590	5	49	theme	RAW	824:826	arg1	cells					834:838	LPS-stimulated RAW 264.7 cells	809:838	LPS-stimulated RAW 264.7 cells	809:838	In addition, EEF6 exhibited potent anti-inflammatory ability against LPS-stimulated RAW 264.7 cells via MAPK/NF-κB signaling pathways by decreasing the secretion of nitric oxide (NO), interleukin (IL)-6, TNF-α, and monocyte chemotactic protein (MCP)-1, inhibiting the translocation of p65 from cytoplasm to nucleus, and suppressing the phosphorylation of ERK, JNK, and p38.
31446590	2	50	theme	R.	353:354	arg1	root					363:366	R. minima root	353:366	R. minima root	353:366	In this study, we examined the anti-inflammatory property of an ethanol fraction (EEF6) from R. minima root on lipopolysaccharide (LPS)-induced RAW 264.7 cells, as well as its underlying mechanism.
31446590	0	51	theme	Rhynchosia	16:25	arg1	root					34:37	Rhynchosia minima root	16:37	Rhynchosia minima root	16:37	Flavonoids from Rhynchosia minima root exerts anti-inflammatory activity in lipopolysaccharide-stimulated RAW 264.7 cells via MAPK/NF-κB signaling pathway.
31446590	3	52	theme	EEF6	486:489	arg1	composition					471:481	The compound composition	458:481	The compound composition of EEF6	458:489	The compound composition of EEF6 was determined by high-performance liquid chromatography-mass spectrometry.
30066486	7	0	theme	wall	1034:1037	arg1	polysaccharides					1039:1053	plant cell wall polysaccharides proteins and lipid degradation and oxidoreduction	1023:1103	polysaccharides	1039:1053	Finally, proteomic profiling of the mutant secretome revealed significant changes in plant cell wall polysaccharides proteins and lipid degradation and oxidoreduction, highlighting the importance of BcPacC in the necrotrophic lifestyle of B. cinerea.
30066486	4	1	theme	host	647:650	arg1	tissue					652:657	the host tissue	643:657	the host tissue	643:657	Characterization of the BcpacC deletion mutant revealed an alteration of both fungal growth and virulence depending on the pH of the culture medium or of the host tissue.
30066486	0	2	theme	Botrytis	60:67	arg1	cinerea					69:75	Botrytis cinerea	60:75	Botrytis cinerea	60:75	The pH regulator PacC: a host-dependent virulence factor in Botrytis cinerea.
30066486	3	3	theme	PacC	462:465	arg1	factor					481:486	the PacC transcription factor	458:486	the PacC transcription factor	458:486	In this work, we examined the Pal/Pac pathway, the fungal ambient pH-responsive signalling circuit, and investigated the role of the PacC transcription factor.
30066486	6	4	dep	production	899:908	arg1	as					892:893	as	892:893	as	892:893	The capacity of the mutant to acidify its environment and, more particularly, to produce oxalic acid was affected, as was production of reactive oxygen species.
30066486	6	4	dep	production	899:908	arg1	was					895:897	was	895:897	was	895:897	The capacity of the mutant to acidify its environment and, more particularly, to produce oxalic acid was affected, as was production of reactive oxygen species.
30066486	7	5	theme	plant	1023:1027	arg1	polysaccharides					1039:1053	plant cell wall polysaccharides proteins and lipid degradation and oxidoreduction	1023:1103	polysaccharides	1039:1053	Finally, proteomic profiling of the mutant secretome revealed significant changes in plant cell wall polysaccharides proteins and lipid degradation and oxidoreduction, highlighting the importance of BcPacC in the necrotrophic lifestyle of B. cinerea.
30066486	1	6	theme	wide	142:145	arg1	plants					158:163	plants	158:163	plants	158:163	The phytopathogenic fungus Botrytis cinerea is able to infect a wide variety of plants and plant tissues with differing chemical compositions.
30066486	1	6	theme	wide	142:145	arg1	tissues					175:181	plant tissues	169:181	plant tissues	169:181	The phytopathogenic fungus Botrytis cinerea is able to infect a wide variety of plants and plant tissues with differing chemical compositions.
30066486	1	6	theme	wide	142:145	arg1	variety					147:153	a wide variety	140:153	a wide variety of plants and plant tissues	140:181	The phytopathogenic fungus Botrytis cinerea is able to infect a wide variety of plants and plant tissues with differing chemical compositions.
30066486	7	7	theme	BcPacC	1137:1142	arg1	importance					1123:1132	the importance	1119:1132	the importance of BcPacC in the necrotrophic lifestyle of B. cinerea	1119:1186	Finally, proteomic profiling of the mutant secretome revealed significant changes in plant cell wall polysaccharides proteins and lipid degradation and oxidoreduction, highlighting the importance of BcPacC in the necrotrophic lifestyle of B. cinerea.
30066486	7	8	from	importance	1123:1132	arg1	lifestyle					1164:1172	the necrotrophic lifestyle	1147:1172	the necrotrophic lifestyle of B. cinerea	1147:1186	Finally, proteomic profiling of the mutant secretome revealed significant changes in plant cell wall polysaccharides proteins and lipid degradation and oxidoreduction, highlighting the importance of BcPacC in the necrotrophic lifestyle of B. cinerea.
30066486	7	9	theme	cell	1029:1032	arg1	polysaccharides					1039:1053	plant cell wall polysaccharides proteins and lipid degradation and oxidoreduction	1023:1103	polysaccharides	1039:1053	Finally, proteomic profiling of the mutant secretome revealed significant changes in plant cell wall polysaccharides proteins and lipid degradation and oxidoreduction, highlighting the importance of BcPacC in the necrotrophic lifestyle of B. cinerea.
30066486	7	10	theme	B.	1177:1178	arg1	cinerea					1180:1186	B. cinerea	1177:1186	B. cinerea	1177:1186	Finally, proteomic profiling of the mutant secretome revealed significant changes in plant cell wall polysaccharides proteins and lipid degradation and oxidoreduction, highlighting the importance of BcPacC in the necrotrophic lifestyle of B. cinerea.
30066486	5	11	theme	mutant	685:690	arg1	pathogenicity					664:676	The pathogenicity	660:676	The pathogenicity of the mutant	660:690	The pathogenicity of the mutant was altered on plants exhibiting a neutral pH and not on plants with acidic tissues.
30066486	4	12	theme	medium	630:635	arg1	pH					612:613	the pH	608:613	the pH of the culture medium or of the host tissue	608:657	Characterization of the BcpacC deletion mutant revealed an alteration of both fungal growth and virulence depending on the pH of the culture medium or of the host tissue.
30066486	3	13	theme	ambient	387:393	arg1	circuit					420:426	the fungal ambient pH-responsive signalling circuit	376:426	the fungal ambient pH-responsive signalling circuit	376:426	In this work, we examined the Pal/Pac pathway, the fungal ambient pH-responsive signalling circuit, and investigated the role of the PacC transcription factor.
30066486	3	13	theme	ambient	387:393	arg1	pathway					367:373	the Pal/Pac pathway	355:373	the Pal/Pac pathway	355:373	In this work, we examined the Pal/Pac pathway, the fungal ambient pH-responsive signalling circuit, and investigated the role of the PacC transcription factor.
30066486	0	14	theme	host-dependent	25:38	arg1	factor					50:55	a host-dependent virulence factor	23:55	The pH regulator PacC: a host-dependent virulence factor in Botrytis cinerea.	0:76	The pH regulator PacC: a host-dependent virulence factor in Botrytis cinerea.
30066486	7	15	theme	secretome	981:989	arg1	profiling					957:965	proteomic profiling	947:965	proteomic profiling of the mutant secretome	947:989	Finally, proteomic profiling of the mutant secretome revealed significant changes in plant cell wall polysaccharides proteins and lipid degradation and oxidoreduction, highlighting the importance of BcPacC in the necrotrophic lifestyle of B. cinerea.
30066486	6	16	theme	species	929:935	arg1	production					899:908	production	899:908	production of reactive oxygen species	899:935	The capacity of the mutant to acidify its environment and, more particularly, to produce oxalic acid was affected, as was production of reactive oxygen species.
30066486	3	17	theme	pH-responsive	395:407	arg1	circuit					420:426	the fungal ambient pH-responsive signalling circuit	376:426	the fungal ambient pH-responsive signalling circuit	376:426	In this work, we examined the Pal/Pac pathway, the fungal ambient pH-responsive signalling circuit, and investigated the role of the PacC transcription factor.
30066486	3	17	theme	pH-responsive	395:407	arg1	pathway					367:373	the Pal/Pac pathway	355:373	the Pal/Pac pathway	355:373	In this work, we examined the Pal/Pac pathway, the fungal ambient pH-responsive signalling circuit, and investigated the role of the PacC transcription factor.
30066486	0	18	theme	pH	4:5	arg1	PacC					17:20	The pH regulator PacC	0:20	The pH regulator PacC: a host-dependent virulence factor in Botrytis cinerea.	0:76	The pH regulator PacC: a host-dependent virulence factor in Botrytis cinerea.
30066486	5	19	with	plants	749:754	arg1	tissues					768:774	acidic tissues	761:774	acidic tissues	761:774	The pathogenicity of the mutant was altered on plants exhibiting a neutral pH and not on plants with acidic tissues.
30066486	1	20	theme	plants	158:163	arg1	plants					158:163	plants	158:163	plants	158:163	The phytopathogenic fungus Botrytis cinerea is able to infect a wide variety of plants and plant tissues with differing chemical compositions.
30066486	1	20	theme	plants	158:163	arg1	tissues					175:181	plant tissues	169:181	plant tissues	169:181	The phytopathogenic fungus Botrytis cinerea is able to infect a wide variety of plants and plant tissues with differing chemical compositions.
30066486	1	20	theme	plants	158:163	arg1	variety					147:153	a wide variety	140:153	a wide variety of plants and plant tissues	140:181	The phytopathogenic fungus Botrytis cinerea is able to infect a wide variety of plants and plant tissues with differing chemical compositions.
30066486	6	21	dep	acidify	807:813	arg1	produce					858:864	produce	858:864	to produce oxalic acid	855:876	The capacity of the mutant to acidify its environment and, more particularly, to produce oxalic acid was affected, as was production of reactive oxygen species.
30066486	4	22	theme	deletion	520:527	arg1	mutant					529:534	the BcpacC deletion mutant	509:534	the BcpacC deletion mutant	509:534	Characterization of the BcpacC deletion mutant revealed an alteration of both fungal growth and virulence depending on the pH of the culture medium or of the host tissue.
30066486	7	23	theme	significant	1000:1010	arg1	changes					1012:1018	significant changes	1000:1018	significant changes in plant cell wall polysaccharides proteins and lipid degradation and oxidoreduction	1000:1103	Finally, proteomic profiling of the mutant secretome revealed significant changes in plant cell wall polysaccharides proteins and lipid degradation and oxidoreduction, highlighting the importance of BcPacC in the necrotrophic lifestyle of B. cinerea.
30066486	2	24	theme	ambient	287:293	arg1	pH					295:296	its ambient pH	283:296	its ambient pH	283:296	During its interaction with the host, this pathogen modulates its ambient pH by secreting acids or ammonia.
30066486	3	25	theme	fungal	380:385	arg1	circuit					420:426	the fungal ambient pH-responsive signalling circuit	376:426	the fungal ambient pH-responsive signalling circuit	376:426	In this work, we examined the Pal/Pac pathway, the fungal ambient pH-responsive signalling circuit, and investigated the role of the PacC transcription factor.
30066486	3	25	theme	fungal	380:385	arg1	pathway					367:373	the Pal/Pac pathway	355:373	the Pal/Pac pathway	355:373	In this work, we examined the Pal/Pac pathway, the fungal ambient pH-responsive signalling circuit, and investigated the role of the PacC transcription factor.
30066486	4	26	theme	BcpacC	513:518	arg1	mutant					529:534	the BcpacC deletion mutant	509:534	the BcpacC deletion mutant	509:534	Characterization of the BcpacC deletion mutant revealed an alteration of both fungal growth and virulence depending on the pH of the culture medium or of the host tissue.
30066486	7	27	theme	mutant	974:979	arg1	secretome					981:989	the mutant secretome	970:989	the mutant secretome	970:989	Finally, proteomic profiling of the mutant secretome revealed significant changes in plant cell wall polysaccharides proteins and lipid degradation and oxidoreduction, highlighting the importance of BcPacC in the necrotrophic lifestyle of B. cinerea.
30066486	1	28	theme	phytopathogenic	82:96	arg1	able					125:128	able	125:128	able	125:128	The phytopathogenic fungus Botrytis cinerea is able to infect a wide variety of plants and plant tissues with differing chemical compositions.
30066486	1	28	theme	phytopathogenic	82:96	arg1	cinerea					114:120	The phytopathogenic fungus Botrytis cinerea	78:120	The phytopathogenic fungus Botrytis cinerea	78:120	The phytopathogenic fungus Botrytis cinerea is able to infect a wide variety of plants and plant tissues with differing chemical compositions.
30066486	1	29	theme	plant	169:173	arg1	tissues					175:181	plant tissues	169:181	plant tissues	169:181	The phytopathogenic fungus Botrytis cinerea is able to infect a wide variety of plants and plant tissues with differing chemical compositions.
30066486	0	30	theme	regulator	7:15	arg1	PacC					17:20	The pH regulator PacC	0:20	The pH regulator PacC: a host-dependent virulence factor in Botrytis cinerea.	0:76	The pH regulator PacC: a host-dependent virulence factor in Botrytis cinerea.
30066486	7	31	from	changes	1012:1018	arg1	degradation					1074:1084	lipid degradation	1068:1084	lipid degradation	1068:1084	Finally, proteomic profiling of the mutant secretome revealed significant changes in plant cell wall polysaccharides proteins and lipid degradation and oxidoreduction, highlighting the importance of BcPacC in the necrotrophic lifestyle of B. cinerea.
30066486	7	31	from	changes	1012:1018	arg1	polysaccharides					1039:1053	plant cell wall polysaccharides proteins and lipid degradation and oxidoreduction	1023:1103	polysaccharides	1039:1053	Finally, proteomic profiling of the mutant secretome revealed significant changes in plant cell wall polysaccharides proteins and lipid degradation and oxidoreduction, highlighting the importance of BcPacC in the necrotrophic lifestyle of B. cinerea.
30066486	4	32	theme	growth	574:579	arg1	alteration					548:557	an alteration	545:557	an alteration of both fungal growth and virulence	545:593	Characterization of the BcpacC deletion mutant revealed an alteration of both fungal growth and virulence depending on the pH of the culture medium or of the host tissue.
30066486	6	33	theme	mutant	797:802	arg1	capacity					781:788	The capacity	777:788	The capacity of the mutant to acidify its environment and, more particularly, to produce oxalic acid	777:876	The capacity of the mutant to acidify its environment and, more particularly, to produce oxalic acid was affected, as was production of reactive oxygen species.
30066486	1	34	theme	fungus	98:103	arg1	able					125:128	able	125:128	able	125:128	The phytopathogenic fungus Botrytis cinerea is able to infect a wide variety of plants and plant tissues with differing chemical compositions.
30066486	1	34	theme	fungus	98:103	arg1	cinerea					114:120	The phytopathogenic fungus Botrytis cinerea	78:120	The phytopathogenic fungus Botrytis cinerea	78:120	The phytopathogenic fungus Botrytis cinerea is able to infect a wide variety of plants and plant tissues with differing chemical compositions.
30066486	1	35	theme	tissues	175:181	arg1	plants					158:163	plants	158:163	plants	158:163	The phytopathogenic fungus Botrytis cinerea is able to infect a wide variety of plants and plant tissues with differing chemical compositions.
30066486	1	35	theme	tissues	175:181	arg1	tissues					175:181	plant tissues	169:181	plant tissues	169:181	The phytopathogenic fungus Botrytis cinerea is able to infect a wide variety of plants and plant tissues with differing chemical compositions.
30066486	1	35	theme	tissues	175:181	arg1	variety					147:153	a wide variety	140:153	a wide variety of plants and plant tissues	140:181	The phytopathogenic fungus Botrytis cinerea is able to infect a wide variety of plants and plant tissues with differing chemical compositions.
30066486	7	36	theme	lipid	1068:1072	arg1	degradation					1074:1084	lipid degradation	1068:1084	lipid degradation	1068:1084	Finally, proteomic profiling of the mutant secretome revealed significant changes in plant cell wall polysaccharides proteins and lipid degradation and oxidoreduction, highlighting the importance of BcPacC in the necrotrophic lifestyle of B. cinerea.
30066486	4	37	theme	mutant	529:534	arg1	Characterization					489:504	Characterization	489:504	Characterization of the BcpacC deletion mutant	489:534	Characterization of the BcpacC deletion mutant revealed an alteration of both fungal growth and virulence depending on the pH of the culture medium or of the host tissue.
30066486	7	38	theme	cinerea	1180:1186	arg1	lifestyle					1164:1172	the necrotrophic lifestyle	1147:1172	the necrotrophic lifestyle of B. cinerea	1147:1186	Finally, proteomic profiling of the mutant secretome revealed significant changes in plant cell wall polysaccharides proteins and lipid degradation and oxidoreduction, highlighting the importance of BcPacC in the necrotrophic lifestyle of B. cinerea.
30066486	4	39	theme	culture	622:628	arg1	medium					630:635	the culture medium	618:635	the culture medium	618:635	Characterization of the BcpacC deletion mutant revealed an alteration of both fungal growth and virulence depending on the pH of the culture medium or of the host tissue.
30066486	3	40	theme	signalling	409:418	arg1	circuit					420:426	the fungal ambient pH-responsive signalling circuit	376:426	the fungal ambient pH-responsive signalling circuit	376:426	In this work, we examined the Pal/Pac pathway, the fungal ambient pH-responsive signalling circuit, and investigated the role of the PacC transcription factor.
30066486	3	40	theme	signalling	409:418	arg1	pathway					367:373	the Pal/Pac pathway	355:373	the Pal/Pac pathway	355:373	In this work, we examined the Pal/Pac pathway, the fungal ambient pH-responsive signalling circuit, and investigated the role of the PacC transcription factor.
30066486	5	41	theme	neutral	727:733	arg1	pH					735:736	a neutral pH	725:736	a neutral pH	725:736	The pathogenicity of the mutant was altered on plants exhibiting a neutral pH and not on plants with acidic tissues.
30066486	2	42	with	interaction	232:242	arg1	host					253:256	the host	249:256	the host	249:256	During its interaction with the host, this pathogen modulates its ambient pH by secreting acids or ammonia.
30066486	6	43	theme	oxygen	922:927	arg1	species					929:935	reactive oxygen species	913:935	reactive oxygen species	913:935	The capacity of the mutant to acidify its environment and, more particularly, to produce oxalic acid was affected, as was production of reactive oxygen species.
30066486	0	44	theme	virulence	40:48	arg1	factor					50:55	a host-dependent virulence factor	23:55	The pH regulator PacC: a host-dependent virulence factor in Botrytis cinerea.	0:76	The pH regulator PacC: a host-dependent virulence factor in Botrytis cinerea.
30066486	6	45	theme	oxalic	866:871	arg1	acid					873:876	oxalic acid	866:876	oxalic acid	866:876	The capacity of the mutant to acidify its environment and, more particularly, to produce oxalic acid was affected, as was production of reactive oxygen species.
30066486	7	46	theme	proteomic	947:955	arg1	profiling					957:965	proteomic profiling	947:965	proteomic profiling of the mutant secretome	947:989	Finally, proteomic profiling of the mutant secretome revealed significant changes in plant cell wall polysaccharides proteins and lipid degradation and oxidoreduction, highlighting the importance of BcPacC in the necrotrophic lifestyle of B. cinerea.
30066486	0	47	from	factor	50:55	arg1	cinerea					69:75	Botrytis cinerea	60:75	Botrytis cinerea	60:75	The pH regulator PacC: a host-dependent virulence factor in Botrytis cinerea.
30066486	1	48	theme	Botrytis	105:112	arg1	able					125:128	able	125:128	able	125:128	The phytopathogenic fungus Botrytis cinerea is able to infect a wide variety of plants and plant tissues with differing chemical compositions.
30066486	1	48	theme	Botrytis	105:112	arg1	cinerea					114:120	The phytopathogenic fungus Botrytis cinerea	78:120	The phytopathogenic fungus Botrytis cinerea	78:120	The phytopathogenic fungus Botrytis cinerea is able to infect a wide variety of plants and plant tissues with differing chemical compositions.
30066486	3	49	theme	Pal/Pac	359:365	arg1	circuit					420:426	the fungal ambient pH-responsive signalling circuit	376:426	the fungal ambient pH-responsive signalling circuit	376:426	In this work, we examined the Pal/Pac pathway, the fungal ambient pH-responsive signalling circuit, and investigated the role of the PacC transcription factor.
30066486	3	49	theme	Pal/Pac	359:365	arg1	pathway					367:373	the Pal/Pac pathway	355:373	the Pal/Pac pathway	355:373	In this work, we examined the Pal/Pac pathway, the fungal ambient pH-responsive signalling circuit, and investigated the role of the PacC transcription factor.
30066486	4	50	theme	tissue	652:657	arg1	pH					612:613	the pH	608:613	the pH of the culture medium or of the host tissue	608:657	Characterization of the BcpacC deletion mutant revealed an alteration of both fungal growth and virulence depending on the pH of the culture medium or of the host tissue.
30066486	6	51	dep	capacity	781:788	arg1	acidify					807:813	acidify	807:813	to acidify its environment	804:829	The capacity of the mutant to acidify its environment and, more particularly, to produce oxalic acid was affected, as was production of reactive oxygen species.
30066486	0	52	dep	PacC	17:20	arg1	factor					50:55	a host-dependent virulence factor	23:55	The pH regulator PacC: a host-dependent virulence factor in Botrytis cinerea.	0:76	The pH regulator PacC: a host-dependent virulence factor in Botrytis cinerea.
30066486	1	53	theme	chemical	198:205	arg1	compositions					207:218	chemical compositions	198:218	chemical compositions	198:218	The phytopathogenic fungus Botrytis cinerea is able to infect a wide variety of plants and plant tissues with differing chemical compositions.
30066486	4	54	theme	fungal	567:572	arg1	growth					574:579	fungal growth	567:579	fungal growth	567:579	Characterization of the BcpacC deletion mutant revealed an alteration of both fungal growth and virulence depending on the pH of the culture medium or of the host tissue.
30066486	7	55	theme	necrotrophic	1151:1162	arg1	lifestyle					1164:1172	the necrotrophic lifestyle	1147:1172	the necrotrophic lifestyle of B. cinerea	1147:1186	Finally, proteomic profiling of the mutant secretome revealed significant changes in plant cell wall polysaccharides proteins and lipid degradation and oxidoreduction, highlighting the importance of BcPacC in the necrotrophic lifestyle of B. cinerea.
30066486	5	56	theme	acidic	761:766	arg1	tissues					768:774	acidic tissues	761:774	acidic tissues	761:774	The pathogenicity of the mutant was altered on plants exhibiting a neutral pH and not on plants with acidic tissues.
30066486	3	57	theme	transcription	467:479	arg1	factor					481:486	the PacC transcription factor	458:486	the PacC transcription factor	458:486	In this work, we examined the Pal/Pac pathway, the fungal ambient pH-responsive signalling circuit, and investigated the role of the PacC transcription factor.
30066486	4	58	theme	virulence	585:593	arg1	alteration					548:557	an alteration	545:557	an alteration of both fungal growth and virulence	545:593	Characterization of the BcpacC deletion mutant revealed an alteration of both fungal growth and virulence depending on the pH of the culture medium or of the host tissue.
30066486	7	59	dep	polysaccharides	1039:1053	arg1	proteins					1055:1062	proteins	1055:1062	proteins	1055:1062	Finally, proteomic profiling of the mutant secretome revealed significant changes in plant cell wall polysaccharides proteins and lipid degradation and oxidoreduction, highlighting the importance of BcPacC in the necrotrophic lifestyle of B. cinerea.
30066486	3	60	theme	factor	481:486	arg1	role					450:453	the role	446:453	the role of the PacC transcription factor	446:486	In this work, we examined the Pal/Pac pathway, the fungal ambient pH-responsive signalling circuit, and investigated the role of the PacC transcription factor.
30066486	6	61	theme	reactive	913:920	arg1	species					929:935	reactive oxygen species	913:935	reactive oxygen species	913:935	The capacity of the mutant to acidify its environment and, more particularly, to produce oxalic acid was affected, as was production of reactive oxygen species.
31275699	7	0	with	of bioaccessible	1189:1204	arg1	antioxidation					1235:1247	antioxidation	1235:1247	antioxidation	1235:1247	Results indicate that CY with favourable swallowing performance can serve as a reliable source of bioaccessible and bioactive compounds with antioxidation.
31275699	1	1	from	information	177:187	arg1	bioaccessibility					214:229	bioaccessibility	214:229	bioaccessibility	214:229	There is dearth of documented information on rheological behavior, bioaccessibility and antioxidant potential of cooked yam flour (CY).
31275699	1	1	from	information	177:187	arg1	potential					247:255	antioxidant potential	235:255	antioxidant potential	235:255	There is dearth of documented information on rheological behavior, bioaccessibility and antioxidant potential of cooked yam flour (CY).
31275699	1	1	from	information	177:187	arg1	behavior					204:211	rheological behavior	192:211	rheological behavior	192:211	There is dearth of documented information on rheological behavior, bioaccessibility and antioxidant potential of cooked yam flour (CY).
31275699	3	2	theme	gel-like	-1:6	arg1	characteristics					515:529	enhanced pseudoplastic and ''gel-like" characteristics	476:529	enhanced pseudoplastic and ''gel-like" characteristics	476:529	CY displayed enhanced pseudoplastic and ''gel-like" characteristics with incremental concentration (4.5-9.0%).
31275699	1	3	theme	yam	267:269	arg1	flour					271:275	cooked yam flour	260:275	cooked yam flour (CY)	260:280	There is dearth of documented information on rheological behavior, bioaccessibility and antioxidant potential of cooked yam flour (CY).
31275699	1	3	theme	yam	267:269	arg1	CY					278:279	CY	278:279	CY	278:279	There is dearth of documented information on rheological behavior, bioaccessibility and antioxidant potential of cooked yam flour (CY).
31275699	0	4	theme	functional	77:86	arg1	components					88:97	functional components	77:97	functional components	77:97	Rheological properties and effects of in vitro gastrointestinal digestion on functional components and antioxidant activities of cooked yam flour.
31275699	0	5	from	properties	12:21	arg1	components					88:97	functional components	77:97	functional components	77:97	Rheological properties and effects of in vitro gastrointestinal digestion on functional components and antioxidant activities of cooked yam flour.
31275699	0	5	from	properties	12:21	arg1	activities					115:124	antioxidant activities	103:124	antioxidant activities	103:124	Rheological properties and effects of in vitro gastrointestinal digestion on functional components and antioxidant activities of cooked yam flour.
31275699	1	6	from	dearth	156:161	arg1	bioaccessibility					214:229	bioaccessibility	214:229	bioaccessibility	214:229	There is dearth of documented information on rheological behavior, bioaccessibility and antioxidant potential of cooked yam flour (CY).
31275699	1	6	from	dearth	156:161	arg1	potential					247:255	antioxidant potential	235:255	antioxidant potential	235:255	There is dearth of documented information on rheological behavior, bioaccessibility and antioxidant potential of cooked yam flour (CY).
31275699	1	6	from	dearth	156:161	arg1	behavior					204:211	rheological behavior	192:211	rheological behavior	192:211	There is dearth of documented information on rheological behavior, bioaccessibility and antioxidant potential of cooked yam flour (CY).
31275699	3	7	theme	"	513:513	arg1	characteristics					515:529	enhanced pseudoplastic and ''gel-like" characteristics	476:529	enhanced pseudoplastic and ''gel-like" characteristics	476:529	CY displayed enhanced pseudoplastic and ''gel-like" characteristics with incremental concentration (4.5-9.0%).
31275699	1	8	theme	flour	271:275	arg1	bioaccessibility					214:229	bioaccessibility	214:229	bioaccessibility	214:229	There is dearth of documented information on rheological behavior, bioaccessibility and antioxidant potential of cooked yam flour (CY).
31275699	1	8	theme	flour	271:275	arg1	potential					247:255	antioxidant potential	235:255	antioxidant potential	235:255	There is dearth of documented information on rheological behavior, bioaccessibility and antioxidant potential of cooked yam flour (CY).
31275699	1	8	theme	flour	271:275	arg1	behavior					204:211	rheological behavior	192:211	rheological behavior	192:211	There is dearth of documented information on rheological behavior, bioaccessibility and antioxidant potential of cooked yam flour (CY).
31275699	0	9	theme	antioxidant	103:113	arg1	activities					115:124	antioxidant activities	103:124	antioxidant activities	103:124	Rheological properties and effects of in vitro gastrointestinal digestion on functional components and antioxidant activities of cooked yam flour.
31275699	5	10	theme	highest	952:958	arg1	content					960:966	highest content	952:966	highest content of 9.81 mg/g for arginine	952:992	Sixteen FAAs including 7 essential amino acids were detected with highest content of 9.81 mg/g for arginine.
31275699	4	11	theme	maximal	725:731	arg1	increment					733:741	maximal increment	725:741	maximal increment of 3.51-fold for TS followed by AP (3.05-fold), and DPPH, ABTS, FRAP and FIC assays pointed to a significant increase in antioxidant activity	725:883	After GID, contents of total polyphenols, flavonoids, sugar (TS), acidic polysaccharides (AP) and free amino acids (FAAs) significantly increased with maximal increment of 3.51-fold for TS followed by AP (3.05-fold), and DPPH, ABTS, FRAP and FIC assays pointed to a significant increase in antioxidant activity.
31275699	7	12	with	compounds	1220:1228	arg1	antioxidation					1235:1247	antioxidation	1235:1247	antioxidation	1235:1247	Results indicate that CY with favourable swallowing performance can serve as a reliable source of bioaccessible and bioactive compounds with antioxidation.
31275699	4	13	theme	acids	683:687	arg1	contents					585:592	contents	585:592	contents of total polyphenols, flavonoids, sugar (TS), acidic polysaccharides (AP) and free amino acids (FAAs)	585:694	After GID, contents of total polyphenols, flavonoids, sugar (TS), acidic polysaccharides (AP) and free amino acids (FAAs) significantly increased with maximal increment of 3.51-fold for TS followed by AP (3.05-fold), and DPPH, ABTS, FRAP and FIC assays pointed to a significant increase in antioxidant activity.
31275699	7	14	theme	favourable swallowing	1124:1144	arg1	performance					1146:1156	favourable swallowing performance	1124:1156	favourable swallowing performance	1124:1156	Results indicate that CY with favourable swallowing performance can serve as a reliable source of bioaccessible and bioactive compounds with antioxidation.
31275699	6	15	theme	scanning	1064:1071	arg1	microscopy					1082:1091	scanning electron microscopy	1064:1091	scanning electron microscopy	1064:1091	Large block remnants with a micro-porous structure were confirmed by scanning electron microscopy.
31275699	2	16	from	properties	334:343	arg1	activities					445:454	antioxidant activities	433:454	antioxidant activities	433:454	This study was carried out to evaluate rheological properties and effects of in vitro gastrointestinal digestion (GID) on functional compositions and antioxidant activities of CY.
31275699	2	16	from	properties	334:343	arg1	compositions					416:427	functional compositions	405:427	functional compositions	405:427	This study was carried out to evaluate rheological properties and effects of in vitro gastrointestinal digestion (GID) on functional compositions and antioxidant activities of CY.
31275699	3	17	theme	incremental	536:546	arg1	concentration					548:560	incremental concentration	536:560	incremental concentration (4.5-9.0%)	536:571	CY displayed enhanced pseudoplastic and ''gel-like" characteristics with incremental concentration (4.5-9.0%).
31275699	3	17	theme	incremental	536:546	arg1	%					570:570	4.5-9.0%	563:570	4.5-9.0%	563:570	CY displayed enhanced pseudoplastic and ''gel-like" characteristics with incremental concentration (4.5-9.0%).
31275699	4	18	theme	polysaccharides	647:661	arg1	contents					585:592	contents	585:592	contents of total polyphenols, flavonoids, sugar (TS), acidic polysaccharides (AP) and free amino acids (FAAs)	585:694	After GID, contents of total polyphenols, flavonoids, sugar (TS), acidic polysaccharides (AP) and free amino acids (FAAs) significantly increased with maximal increment of 3.51-fold for TS followed by AP (3.05-fold), and DPPH, ABTS, FRAP and FIC assays pointed to a significant increase in antioxidant activity.
31275699	1	19	theme	documented	166:175	arg1	information					177:187	documented information	166:187	documented information on rheological behavior, bioaccessibility and antioxidant potential of cooked yam flour (CY)	166:280	There is dearth of documented information on rheological behavior, bioaccessibility and antioxidant potential of cooked yam flour (CY).
31275699	2	20	dep	in	360:361	arg1	vitro					363:367	vitro	363:367	vitro	363:367	This study was carried out to evaluate rheological properties and effects of in vitro gastrointestinal digestion (GID) on functional compositions and antioxidant activities of CY.
31275699	4	21	theme	polyphenols	603:613	arg1	contents					585:592	contents	585:592	contents of total polyphenols, flavonoids, sugar (TS), acidic polysaccharides (AP) and free amino acids (FAAs)	585:694	After GID, contents of total polyphenols, flavonoids, sugar (TS), acidic polysaccharides (AP) and free amino acids (FAAs) significantly increased with maximal increment of 3.51-fold for TS followed by AP (3.05-fold), and DPPH, ABTS, FRAP and FIC assays pointed to a significant increase in antioxidant activity.
31275699	1	22	theme	information	177:187	arg1	dearth					156:161	dearth	156:161	dearth of documented information on rheological behavior, bioaccessibility and antioxidant potential of cooked yam flour (CY)	156:280	There is dearth of documented information on rheological behavior, bioaccessibility and antioxidant potential of cooked yam flour (CY).
31275699	2	23	from	effects	349:355	arg1	activities					445:454	antioxidant activities	433:454	antioxidant activities	433:454	This study was carried out to evaluate rheological properties and effects of in vitro gastrointestinal digestion (GID) on functional compositions and antioxidant activities of CY.
31275699	2	23	from	effects	349:355	arg1	compositions					416:427	functional compositions	405:427	functional compositions	405:427	This study was carried out to evaluate rheological properties and effects of in vitro gastrointestinal digestion (GID) on functional compositions and antioxidant activities of CY.
31275699	2	24	theme	digestion	386:394	arg1	properties					334:343	rheological properties	322:343	rheological properties	322:343	This study was carried out to evaluate rheological properties and effects of in vitro gastrointestinal digestion (GID) on functional compositions and antioxidant activities of CY.
31275699	2	24	theme	digestion	386:394	arg1	effects					349:355	effects	349:355	effects of in vitro gastrointestinal digestion (GID) on functional compositions and antioxidant activities of CY	349:460	This study was carried out to evaluate rheological properties and effects of in vitro gastrointestinal digestion (GID) on functional compositions and antioxidant activities of CY.
31275699	0	25	theme	Rheological	0:10	arg1	properties					12:21	Rheological properties	0:21	Rheological properties	0:21	Rheological properties and effects of in vitro gastrointestinal digestion on functional components and antioxidant activities of cooked yam flour.
31275699	1	26	theme	rheological	192:202	arg1	behavior					204:211	rheological behavior	192:211	rheological behavior	192:211	There is dearth of documented information on rheological behavior, bioaccessibility and antioxidant potential of cooked yam flour (CY).
31275699	2	27	theme	antioxidant	433:443	arg1	activities					445:454	antioxidant activities	433:454	antioxidant activities	433:454	This study was carried out to evaluate rheological properties and effects of in vitro gastrointestinal digestion (GID) on functional compositions and antioxidant activities of CY.
31275699	0	28	theme	yam	136:138	arg1	flour					140:144	cooked yam flour	129:144	cooked yam flour	129:144	Rheological properties and effects of in vitro gastrointestinal digestion on functional components and antioxidant activities of cooked yam flour.
31275699	7	29	with	CY	1116:1117	arg1	performance					1146:1156	favourable swallowing performance	1124:1156	favourable swallowing performance	1124:1156	Results indicate that CY with favourable swallowing performance can serve as a reliable source of bioaccessible and bioactive compounds with antioxidation.
31275699	4	30	theme	sugar	628:632	arg1	contents					585:592	contents	585:592	contents of total polyphenols, flavonoids, sugar (TS), acidic polysaccharides (AP) and free amino acids (FAAs)	585:694	After GID, contents of total polyphenols, flavonoids, sugar (TS), acidic polysaccharides (AP) and free amino acids (FAAs) significantly increased with maximal increment of 3.51-fold for TS followed by AP (3.05-fold), and DPPH, ABTS, FRAP and FIC assays pointed to a significant increase in antioxidant activity.
31275699	0	31	theme	cooked	129:134	arg1	flour					140:144	cooked yam flour	129:144	cooked yam flour	129:144	Rheological properties and effects of in vitro gastrointestinal digestion on functional components and antioxidant activities of cooked yam flour.
31275699	4	32	theme	significant	840:850	arg1	increase					852:859	a significant increase	838:859	a significant increase in antioxidant activity	838:883	After GID, contents of total polyphenols, flavonoids, sugar (TS), acidic polysaccharides (AP) and free amino acids (FAAs) significantly increased with maximal increment of 3.51-fold for TS followed by AP (3.05-fold), and DPPH, ABTS, FRAP and FIC assays pointed to a significant increase in antioxidant activity.
31275699	6	33	theme	electron	1073:1080	arg1	microscopy					1082:1091	scanning electron microscopy	1064:1091	scanning electron microscopy	1064:1091	Large block remnants with a micro-porous structure were confirmed by scanning electron microscopy.
31275699	2	34	theme	functional	405:414	arg1	compositions					416:427	functional compositions	405:427	functional compositions	405:427	This study was carried out to evaluate rheological properties and effects of in vitro gastrointestinal digestion (GID) on functional compositions and antioxidant activities of CY.
31275699	0	35	theme	in	38:39	arg1	digestion					64:72	in vitro gastrointestinal digestion	38:72	in vitro gastrointestinal digestion	38:72	Rheological properties and effects of in vitro gastrointestinal digestion on functional components and antioxidant activities of cooked yam flour.
31275699	4	36	theme	free	672:675	arg1	FAAs					690:693	FAAs	690:693	FAAs	690:693	After GID, contents of total polyphenols, flavonoids, sugar (TS), acidic polysaccharides (AP) and free amino acids (FAAs) significantly increased with maximal increment of 3.51-fold for TS followed by AP (3.05-fold), and DPPH, ABTS, FRAP and FIC assays pointed to a significant increase in antioxidant activity.
31275699	4	36	theme	free	672:675	arg1	acids					683:687	free amino acids	672:687	free amino acids (FAAs)	672:694	After GID, contents of total polyphenols, flavonoids, sugar (TS), acidic polysaccharides (AP) and free amino acids (FAAs) significantly increased with maximal increment of 3.51-fold for TS followed by AP (3.05-fold), and DPPH, ABTS, FRAP and FIC assays pointed to a significant increase in antioxidant activity.
31275699	2	37	theme	gastrointestinal	369:384	arg1	GID					397:399	GID	397:399	GID	397:399	This study was carried out to evaluate rheological properties and effects of in vitro gastrointestinal digestion (GID) on functional compositions and antioxidant activities of CY.
31275699	2	37	theme	gastrointestinal	369:384	arg1	digestion					386:394	gastrointestinal digestion	369:394	in vitro gastrointestinal digestion (GID)	360:400	This study was carried out to evaluate rheological properties and effects of in vitro gastrointestinal digestion (GID) on functional compositions and antioxidant activities of CY.
31275699	0	38	theme	flour	140:144	arg1	components					88:97	functional components	77:97	functional components	77:97	Rheological properties and effects of in vitro gastrointestinal digestion on functional components and antioxidant activities of cooked yam flour.
31275699	0	38	theme	flour	140:144	arg1	activities					115:124	antioxidant activities	103:124	antioxidant activities	103:124	Rheological properties and effects of in vitro gastrointestinal digestion on functional components and antioxidant activities of cooked yam flour.
31275699	6	39	with	remnants	1007:1014	arg1	structure					1036:1044	a micro-porous structure	1021:1044	a micro-porous structure	1021:1044	Large block remnants with a micro-porous structure were confirmed by scanning electron microscopy.
31275699	1	40	from	bioaccessibility	214:229	arg1	dearth					156:161	dearth	156:161	dearth of documented information on rheological behavior, bioaccessibility and antioxidant potential of cooked yam flour (CY)	156:280	There is dearth of documented information on rheological behavior, bioaccessibility and antioxidant potential of cooked yam flour (CY).
31275699	6	41	theme	Large	995:999	arg1	remnants					1007:1014	Large block remnants	995:1014	Large block remnants with a micro-porous structure	995:1044	Large block remnants with a micro-porous structure were confirmed by scanning electron microscopy.
31275699	0	42	theme	gastrointestinal	47:62	arg1	digestion					64:72	in vitro gastrointestinal digestion	38:72	in vitro gastrointestinal digestion	38:72	Rheological properties and effects of in vitro gastrointestinal digestion on functional components and antioxidant activities of cooked yam flour.
31275699	4	43	theme	antioxidant	864:874	arg1	activity					876:883	antioxidant activity	864:883	antioxidant activity	864:883	After GID, contents of total polyphenols, flavonoids, sugar (TS), acidic polysaccharides (AP) and free amino acids (FAAs) significantly increased with maximal increment of 3.51-fold for TS followed by AP (3.05-fold), and DPPH, ABTS, FRAP and FIC assays pointed to a significant increase in antioxidant activity.
31275699	4	44	theme	FIC	816:818	arg1	assays					820:825	FIC assays	816:825	FIC assays	816:825	After GID, contents of total polyphenols, flavonoids, sugar (TS), acidic polysaccharides (AP) and free amino acids (FAAs) significantly increased with maximal increment of 3.51-fold for TS followed by AP (3.05-fold), and DPPH, ABTS, FRAP and FIC assays pointed to a significant increase in antioxidant activity.
31275699	7	45	theme	source	1182:1187	arg1	of bioaccessible					1189:1204	a reliable source of bioaccessible	1171:1204	a reliable source of bioaccessible	1171:1204	Results indicate that CY with favourable swallowing performance can serve as a reliable source of bioaccessible and bioactive compounds with antioxidation.
31275699	7	45	theme	source	1182:1187	arg1	CY					1116:1117	CY	1116:1117	CY with favourable swallowing performance	1116:1156	Results indicate that CY with favourable swallowing performance can serve as a reliable source of bioaccessible and bioactive compounds with antioxidation.
31275699	0	46	dep	in	38:39	arg1	vitro					41:45	vitro	41:45	vitro	41:45	Rheological properties and effects of in vitro gastrointestinal digestion on functional components and antioxidant activities of cooked yam flour.
31275699	4	47	theme	amino	677:681	arg1	FAAs					690:693	FAAs	690:693	FAAs	690:693	After GID, contents of total polyphenols, flavonoids, sugar (TS), acidic polysaccharides (AP) and free amino acids (FAAs) significantly increased with maximal increment of 3.51-fold for TS followed by AP (3.05-fold), and DPPH, ABTS, FRAP and FIC assays pointed to a significant increase in antioxidant activity.
31275699	4	47	theme	amino	677:681	arg1	acids					683:687	free amino acids	672:687	free amino acids (FAAs)	672:694	After GID, contents of total polyphenols, flavonoids, sugar (TS), acidic polysaccharides (AP) and free amino acids (FAAs) significantly increased with maximal increment of 3.51-fold for TS followed by AP (3.05-fold), and DPPH, ABTS, FRAP and FIC assays pointed to a significant increase in antioxidant activity.
31275699	2	48	theme	CY	459:460	arg1	activities					445:454	antioxidant activities	433:454	antioxidant activities	433:454	This study was carried out to evaluate rheological properties and effects of in vitro gastrointestinal digestion (GID) on functional compositions and antioxidant activities of CY.
31275699	2	48	theme	CY	459:460	arg1	compositions					416:427	functional compositions	405:427	functional compositions	405:427	This study was carried out to evaluate rheological properties and effects of in vitro gastrointestinal digestion (GID) on functional compositions and antioxidant activities of CY.
31275699	4	49	from	increase	852:859	arg1	activity					876:883	antioxidant activity	864:883	antioxidant activity	864:883	After GID, contents of total polyphenols, flavonoids, sugar (TS), acidic polysaccharides (AP) and free amino acids (FAAs) significantly increased with maximal increment of 3.51-fold for TS followed by AP (3.05-fold), and DPPH, ABTS, FRAP and FIC assays pointed to a significant increase in antioxidant activity.
31275699	5	50	theme	9.81 mg/g	971:979	arg1	content					960:966	highest content	952:966	highest content of 9.81 mg/g for arginine	952:992	Sixteen FAAs including 7 essential amino acids were detected with highest content of 9.81 mg/g for arginine.
31275699	1	51	from	behavior	204:211	arg1	dearth					156:161	dearth	156:161	dearth of documented information on rheological behavior, bioaccessibility and antioxidant potential of cooked yam flour (CY)	156:280	There is dearth of documented information on rheological behavior, bioaccessibility and antioxidant potential of cooked yam flour (CY).
31275699	3	52	theme	"	513:513	arg1	characteristics					515:529	enhanced pseudoplastic and ''gel-like" characteristics	476:529	enhanced pseudoplastic and ''gel-like" characteristics	476:529	CY displayed enhanced pseudoplastic and ''gel-like" characteristics with incremental concentration (4.5-9.0%).
31275699	4	53	theme	acidic	640:645	arg1	AP					664:665	AP	664:665	AP	664:665	After GID, contents of total polyphenols, flavonoids, sugar (TS), acidic polysaccharides (AP) and free amino acids (FAAs) significantly increased with maximal increment of 3.51-fold for TS followed by AP (3.05-fold), and DPPH, ABTS, FRAP and FIC assays pointed to a significant increase in antioxidant activity.
31275699	4	53	theme	acidic	640:645	arg1	polysaccharides					647:661	acidic polysaccharides	640:661	acidic polysaccharides (AP)	640:666	After GID, contents of total polyphenols, flavonoids, sugar (TS), acidic polysaccharides (AP) and free amino acids (FAAs) significantly increased with maximal increment of 3.51-fold for TS followed by AP (3.05-fold), and DPPH, ABTS, FRAP and FIC assays pointed to a significant increase in antioxidant activity.
31275699	5	54	theme	essential	911:919	arg1	acids					927:931	7 essential amino acids	909:931	7 essential amino acids	909:931	Sixteen FAAs including 7 essential amino acids were detected with highest content of 9.81 mg/g for arginine.
31275699	2	55	theme	rheological	322:332	arg1	properties					334:343	rheological properties	322:343	rheological properties	322:343	This study was carried out to evaluate rheological properties and effects of in vitro gastrointestinal digestion (GID) on functional compositions and antioxidant activities of CY.
31275699	1	56	theme	antioxidant	235:245	arg1	potential					247:255	antioxidant potential	235:255	antioxidant potential	235:255	There is dearth of documented information on rheological behavior, bioaccessibility and antioxidant potential of cooked yam flour (CY).
31275699	1	57	from	potential	247:255	arg1	dearth					156:161	dearth	156:161	dearth of documented information on rheological behavior, bioaccessibility and antioxidant potential of cooked yam flour (CY)	156:280	There is dearth of documented information on rheological behavior, bioaccessibility and antioxidant potential of cooked yam flour (CY).
31275699	5	58	theme	amino	921:925	arg1	acids					927:931	7 essential amino acids	909:931	7 essential amino acids	909:931	Sixteen FAAs including 7 essential amino acids were detected with highest content of 9.81 mg/g for arginine.
31275699	3	59	theme	pseudoplastic	485:497	arg1	characteristics					515:529	enhanced pseudoplastic and ''gel-like" characteristics	476:529	enhanced pseudoplastic and ''gel-like" characteristics	476:529	CY displayed enhanced pseudoplastic and ''gel-like" characteristics with incremental concentration (4.5-9.0%).
31275699	3	60	theme	enhanced	476:483	arg1	characteristics					515:529	enhanced pseudoplastic and ''gel-like" characteristics	476:529	enhanced pseudoplastic and ''gel-like" characteristics	476:529	CY displayed enhanced pseudoplastic and ''gel-like" characteristics with incremental concentration (4.5-9.0%).
31275699	7	61	theme	reliable	1173:1180	arg1	of bioaccessible					1189:1204	a reliable source of bioaccessible	1171:1204	a reliable source of bioaccessible	1171:1204	Results indicate that CY with favourable swallowing performance can serve as a reliable source of bioaccessible and bioactive compounds with antioxidation.
31275699	7	61	theme	reliable	1173:1180	arg1	CY					1116:1117	CY	1116:1117	CY with favourable swallowing performance	1116:1156	Results indicate that CY with favourable swallowing performance can serve as a reliable source of bioaccessible and bioactive compounds with antioxidation.
31275699	6	62	theme	micro-porous	1023:1034	arg1	structure					1036:1044	a micro-porous structure	1021:1044	a micro-porous structure	1021:1044	Large block remnants with a micro-porous structure were confirmed by scanning electron microscopy.
31275699	7	63	theme	bioactive	1210:1218	arg1	compounds					1220:1228	bioactive compounds	1210:1228	bioactive compounds with antioxidation	1210:1247	Results indicate that CY with favourable swallowing performance can serve as a reliable source of bioaccessible and bioactive compounds with antioxidation.
31275699	7	63	theme	bioactive	1210:1218	arg1	CY					1116:1117	CY	1116:1117	CY with favourable swallowing performance	1116:1156	Results indicate that CY with favourable swallowing performance can serve as a reliable source of bioaccessible and bioactive compounds with antioxidation.
31275699	6	64	theme	block	1001:1005	arg1	remnants					1007:1014	Large block remnants	995:1014	Large block remnants with a micro-porous structure	995:1044	Large block remnants with a micro-porous structure were confirmed by scanning electron microscopy.
31275699	4	65	theme	flavonoids	616:625	arg1	contents					585:592	contents	585:592	contents of total polyphenols, flavonoids, sugar (TS), acidic polysaccharides (AP) and free amino acids (FAAs)	585:694	After GID, contents of total polyphenols, flavonoids, sugar (TS), acidic polysaccharides (AP) and free amino acids (FAAs) significantly increased with maximal increment of 3.51-fold for TS followed by AP (3.05-fold), and DPPH, ABTS, FRAP and FIC assays pointed to a significant increase in antioxidant activity.
31275699	0	66	theme	digestion	64:72	arg1	effects					27:33	effects	27:33	effects of in vitro gastrointestinal digestion on functional components and antioxidant activities of cooked yam flour	27:144	Rheological properties and effects of in vitro gastrointestinal digestion on functional components and antioxidant activities of cooked yam flour.
31275699	0	66	theme	digestion	64:72	arg1	properties					12:21	Rheological properties	0:21	Rheological properties	0:21	Rheological properties and effects of in vitro gastrointestinal digestion on functional components and antioxidant activities of cooked yam flour.
31275699	0	67	from	effects	27:33	arg1	components					88:97	functional components	77:97	functional components	77:97	Rheological properties and effects of in vitro gastrointestinal digestion on functional components and antioxidant activities of cooked yam flour.
31275699	0	67	from	effects	27:33	arg1	activities					115:124	antioxidant activities	103:124	antioxidant activities	103:124	Rheological properties and effects of in vitro gastrointestinal digestion on functional components and antioxidant activities of cooked yam flour.
31275699	4	68	theme	total	597:601	arg1	polyphenols					603:613	total polyphenols	597:613	total polyphenols	597:613	After GID, contents of total polyphenols, flavonoids, sugar (TS), acidic polysaccharides (AP) and free amino acids (FAAs) significantly increased with maximal increment of 3.51-fold for TS followed by AP (3.05-fold), and DPPH, ABTS, FRAP and FIC assays pointed to a significant increase in antioxidant activity.
31275699	2	69	theme	in	360:361	arg1	GID					397:399	GID	397:399	GID	397:399	This study was carried out to evaluate rheological properties and effects of in vitro gastrointestinal digestion (GID) on functional compositions and antioxidant activities of CY.
31275699	2	69	theme	in	360:361	arg1	digestion					386:394	gastrointestinal digestion	369:394	in vitro gastrointestinal digestion (GID)	360:400	This study was carried out to evaluate rheological properties and effects of in vitro gastrointestinal digestion (GID) on functional compositions and antioxidant activities of CY.
31275699	1	70	theme	cooked	260:265	arg1	flour					271:275	cooked yam flour	260:275	cooked yam flour (CY)	260:280	There is dearth of documented information on rheological behavior, bioaccessibility and antioxidant potential of cooked yam flour (CY).
31275699	1	70	theme	cooked	260:265	arg1	CY					278:279	CY	278:279	CY	278:279	There is dearth of documented information on rheological behavior, bioaccessibility and antioxidant potential of cooked yam flour (CY).
29321567	0	0	theme	glycocalyx	74:83	arg1	bush					28:31	bush	28:31	bush	28:31	Structure and elasticity of bush and brush-like models of the endothelial glycocalyx.
29321567	0	0	theme	glycocalyx	74:83	arg1	models					48:53	brush-like models	37:53	brush-like models	37:53	Structure and elasticity of bush and brush-like models of the endothelial glycocalyx.
29321567	6	1	theme	normal	1034:1039	arg1	stress					1041:1046	the normal stress	1030:1046	the normal stress	1030:1046	We show that variations in the glycan density determine the elasticity of the EG for small deformations, and that the normal stress may be effectively dampened by the EG layer, preventing the stress from being transferred to the cell membrane.
29321567	6	1	theme	normal	1034:1039	arg1	dampened					1067:1074	dampened	1067:1074	dampened	1067:1074	We show that variations in the glycan density determine the elasticity of the EG for small deformations, and that the normal stress may be effectively dampened by the EG layer, preventing the stress from being transferred to the cell membrane.
29321567	0	2	theme	endothelial	62:72	arg1	glycocalyx					74:83	the endothelial glycocalyx	58:83	the endothelial glycocalyx	58:83	Structure and elasticity of bush and brush-like models of the endothelial glycocalyx.
29321567	6	3	theme	glycan	947:952	arg1	density					954:960	the glycan density	943:960	the glycan density	943:960	We show that variations in the glycan density determine the elasticity of the EG for small deformations, and that the normal stress may be effectively dampened by the EG layer, preventing the stress from being transferred to the cell membrane.
29321567	7	4	dep	forces	1219:1224	arg1	the					1215:1217	the	1215:1217	the	1215:1217	Furthermore, our bush-like model allows us to evaluate the forces and energies required to overcome the mechanical resistance of the EG.
29321567	2	5	theme	chemical	252:259	arg1	composition					261:271	the chemical composition	248:271	the chemical composition of the EG	248:281	Although the chemical composition of the EG is fairly well known, there is no consensus regarding its ultrastructure.
29321567	6	6	theme	EG	994:995	arg1	elasticity					976:985	the elasticity	972:985	the elasticity of the EG for small deformations	972:1018	We show that variations in the glycan density determine the elasticity of the EG for small deformations, and that the normal stress may be effectively dampened by the EG layer, preventing the stress from being transferred to the cell membrane.
29321567	1	7	theme	vascular	222:229	arg1	system					231:236	the vascular system	218:236	the vascular system	218:236	The endothelial glycocalyx (EG), a sugar-rich layer that lines the luminal surface of blood vessels, is an important constituent of the vascular system.
29321567	3	8	theme	molecular	492:500	arg1	organisation					502:513	its molecular organisation	488:513	its molecular organisation	488:513	While previous experiments probed the properties of the layer at the continuum level, they did not provide sufficient insight into its molecular organisation.
29321567	1	9	theme	system	231:236	arg1	constituent					203:213	an important constituent	190:213	an important constituent of the vascular system	190:236	The endothelial glycocalyx (EG), a sugar-rich layer that lines the luminal surface of blood vessels, is an important constituent of the vascular system.
29321567	1	9	theme	system	231:236	arg1	glycocalyx					102:111	endothelial glycocalyx	90:111	The endothelial glycocalyx (EG)	86:116	The endothelial glycocalyx (EG), a sugar-rich layer that lines the luminal surface of blood vessels, is an important constituent of the vascular system.
29321567	7	10	theme	bush-like	1177:1185	arg1	model					1187:1191	our bush-like model	1173:1191	our bush-like model	1173:1191	Furthermore, our bush-like model allows us to evaluate the forces and energies required to overcome the mechanical resistance of the EG.
29321567	4	11	theme	simple	572:577	arg1	models					610:615	two simple brush and bush-like simulation models	568:615	two simple brush and bush-like simulation models	568:615	In this work, we investigate the EG mechanics using two simple brush and bush-like simulation models, and use these models to describe its molecular structure and elastic response to indentation.
29321567	5	12	theme	mechanical	752:761	arg1	rigidity					855:862	the filament bending rigidity	834:862	the filament bending rigidity	834:862	We analyse the relationship between the mechanical properties of the EG layer and several molecular parameters, including the filament bending rigidity, grafting density, and the type of ultrastructure .
29321567	5	12	theme	mechanical	752:761	arg1	properties					763:772	the mechanical properties	748:772	the mechanical properties of the EG layer	748:788	We analyse the relationship between the mechanical properties of the EG layer and several molecular parameters, including the filament bending rigidity, grafting density, and the type of ultrastructure .
29321567	6	13	theme	EG	1083:1084	arg1	layer					1086:1090	the EG layer	1079:1090	the EG layer	1079:1090	We show that variations in the glycan density determine the elasticity of the EG for small deformations, and that the normal stress may be effectively dampened by the EG layer, preventing the stress from being transferred to the cell membrane.
29321567	4	14	theme	simulation	599:608	arg1	models					610:615	two simple brush and bush-like simulation models	568:615	two simple brush and bush-like simulation models	568:615	In this work, we investigate the EG mechanics using two simple brush and bush-like simulation models, and use these models to describe its molecular structure and elastic response to indentation.
29321567	2	15	theme	EG	280:281	arg1	composition					261:271	the chemical composition	248:271	the chemical composition of the EG	248:281	Although the chemical composition of the EG is fairly well known, there is no consensus regarding its ultrastructure.
29321567	1	16	theme	luminal	153:159	arg1	surface					161:167	the luminal surface	149:167	the luminal surface of blood vessels	149:184	The endothelial glycocalyx (EG), a sugar-rich layer that lines the luminal surface of blood vessels, is an important constituent of the vascular system.
29321567	4	17	theme	molecular structure	655:673	arg1	response					687:694	its molecular structure and elastic response	651:694	its molecular structure and elastic response to indentation	651:709	In this work, we investigate the EG mechanics using two simple brush and bush-like simulation models, and use these models to describe its molecular structure and elastic response to indentation.
29321567	4	18	theme	elastic	679:685	arg1	response					687:694	its molecular structure and elastic response	651:694	its molecular structure and elastic response to indentation	651:709	In this work, we investigate the EG mechanics using two simple brush and bush-like simulation models, and use these models to describe its molecular structure and elastic response to indentation.
29321567	5	19	theme	several	794:800	arg1	rigidity					855:862	the filament bending rigidity	834:862	the filament bending rigidity	834:862	We analyse the relationship between the mechanical properties of the EG layer and several molecular parameters, including the filament bending rigidity, grafting density, and the type of ultrastructure .
29321567	5	19	theme	several	794:800	arg1	parameters					812:821	several molecular parameters	794:821	several molecular parameters	794:821	We analyse the relationship between the mechanical properties of the EG layer and several molecular parameters, including the filament bending rigidity, grafting density, and the type of ultrastructure .
29321567	5	20	theme	and the	883:889	arg1	rigidity					855:862	the filament bending rigidity	834:862	the filament bending rigidity	834:862	We analyse the relationship between the mechanical properties of the EG layer and several molecular parameters, including the filament bending rigidity, grafting density, and the type of ultrastructure .
29321567	5	20	theme	and the	883:889	arg1	type					891:894	and the type	883:894	and the type	883:894	We analyse the relationship between the mechanical properties of the EG layer and several molecular parameters, including the filament bending rigidity, grafting density, and the type of ultrastructure .
29321567	1	21	theme	endothelial	90:100	arg1	constituent					203:213	an important constituent	190:213	an important constituent of the vascular system	190:236	The endothelial glycocalyx (EG), a sugar-rich layer that lines the luminal surface of blood vessels, is an important constituent of the vascular system.
29321567	1	21	theme	endothelial	90:100	arg1	layer					132:136	a sugar-rich layer	119:136	a sugar-rich layer that lines the luminal surface of blood vessels	119:184	The endothelial glycocalyx (EG), a sugar-rich layer that lines the luminal surface of blood vessels, is an important constituent of the vascular system.
29321567	1	21	theme	endothelial	90:100	arg1	EG					114:115	EG	114:115	EG	114:115	The endothelial glycocalyx (EG), a sugar-rich layer that lines the luminal surface of blood vessels, is an important constituent of the vascular system.
29321567	1	21	theme	endothelial	90:100	arg1	glycocalyx					102:111	endothelial glycocalyx	90:111	The endothelial glycocalyx (EG)	86:116	The endothelial glycocalyx (EG), a sugar-rich layer that lines the luminal surface of blood vessels, is an important constituent of the vascular system.
29321567	4	22	theme	brush	579:583	arg1	models					610:615	two simple brush and bush-like simulation models	568:615	two simple brush and bush-like simulation models	568:615	In this work, we investigate the EG mechanics using two simple brush and bush-like simulation models, and use these models to describe its molecular structure and elastic response to indentation.
29321567	5	23	theme	EG	781:782	arg1	layer					784:788	the EG layer	777:788	the EG layer	777:788	We analyse the relationship between the mechanical properties of the EG layer and several molecular parameters, including the filament bending rigidity, grafting density, and the type of ultrastructure .
29321567	1	24	theme	blood	172:176	arg1	vessels					178:184	blood vessels	172:184	blood vessels	172:184	The endothelial glycocalyx (EG), a sugar-rich layer that lines the luminal surface of blood vessels, is an important constituent of the vascular system.
29321567	3	25	theme	previous	363:370	arg1	experiments					372:382	previous experiments	363:382	previous experiments	363:382	While previous experiments probed the properties of the layer at the continuum level, they did not provide sufficient insight into its molecular organisation.
29321567	6	26	from	variations	929:938	arg1	density					954:960	the glycan density	943:960	the glycan density	943:960	We show that variations in the glycan density determine the elasticity of the EG for small deformations, and that the normal stress may be effectively dampened by the EG layer, preventing the stress from being transferred to the cell membrane.
29321567	4	27	theme	bush-like	589:597	arg1	models					610:615	two simple brush and bush-like simulation models	568:615	two simple brush and bush-like simulation models	568:615	In this work, we investigate the EG mechanics using two simple brush and bush-like simulation models, and use these models to describe its molecular structure and elastic response to indentation.
29321567	1	28	theme	vessels	178:184	arg1	surface					161:167	the luminal surface	149:167	the luminal surface of blood vessels	149:184	The endothelial glycocalyx (EG), a sugar-rich layer that lines the luminal surface of blood vessels, is an important constituent of the vascular system.
29321567	0	29	theme	bush	28:31	arg1	elasticity					14:23	elasticity	14:23	elasticity	14:23	Structure and elasticity of bush and brush-like models of the endothelial glycocalyx.
29321567	0	29	theme	bush	28:31	arg1	Structure					0:8	Structure	0:8	Structure	0:8	Structure and elasticity of bush and brush-like models of the endothelial glycocalyx.
29321567	5	30	theme	molecular	802:810	arg1	rigidity					855:862	the filament bending rigidity	834:862	the filament bending rigidity	834:862	We analyse the relationship between the mechanical properties of the EG layer and several molecular parameters, including the filament bending rigidity, grafting density, and the type of ultrastructure .
29321567	5	30	theme	molecular	802:810	arg1	parameters					812:821	several molecular parameters	794:821	several molecular parameters	794:821	We analyse the relationship between the mechanical properties of the EG layer and several molecular parameters, including the filament bending rigidity, grafting density, and the type of ultrastructure .
29321567	3	31	theme	layer	413:417	arg1	properties					395:404	the properties	391:404	the properties of the layer	391:417	While previous experiments probed the properties of the layer at the continuum level, they did not provide sufficient insight into its molecular organisation.
29321567	0	32	theme	models	48:53	arg1	elasticity					14:23	elasticity	14:23	elasticity	14:23	Structure and elasticity of bush and brush-like models of the endothelial glycocalyx.
29321567	0	32	theme	models	48:53	arg1	Structure					0:8	Structure	0:8	Structure	0:8	Structure and elasticity of bush and brush-like models of the endothelial glycocalyx.
29321567	7	33	theme	EG	1293:1294	arg1	resistance					1275:1284	the mechanical resistance	1260:1284	the mechanical resistance of the EG	1260:1294	Furthermore, our bush-like model allows us to evaluate the forces and energies required to overcome the mechanical resistance of the EG.
29321567	5	34	theme	grafting	865:872	arg1	rigidity					855:862	the filament bending rigidity	834:862	the filament bending rigidity	834:862	We analyse the relationship between the mechanical properties of the EG layer and several molecular parameters, including the filament bending rigidity, grafting density, and the type of ultrastructure .
29321567	5	34	theme	grafting	865:872	arg1	density					874:880	grafting density	865:880	grafting density	865:880	We analyse the relationship between the mechanical properties of the EG layer and several molecular parameters, including the filament bending rigidity, grafting density, and the type of ultrastructure .
29321567	0	35	theme	brush-like	37:46	arg1	models					48:53	brush-like models	37:53	brush-like models	37:53	Structure and elasticity of bush and brush-like models of the endothelial glycocalyx.
29321567	6	36	theme	cell	1145:1148	arg1	membrane					1150:1157	the cell membrane	1141:1157	the cell membrane	1141:1157	We show that variations in the glycan density determine the elasticity of the EG for small deformations, and that the normal stress may be effectively dampened by the EG layer, preventing the stress from being transferred to the cell membrane.
29321567	3	37	theme	continuum	426:434	arg1	level					436:440	the continuum level	422:440	the continuum level	422:440	While previous experiments probed the properties of the layer at the continuum level, they did not provide sufficient insight into its molecular organisation.
29321567	5	38	theme	ultrastructure	899:912	arg1	rigidity					855:862	the filament bending rigidity	834:862	the filament bending rigidity	834:862	We analyse the relationship between the mechanical properties of the EG layer and several molecular parameters, including the filament bending rigidity, grafting density, and the type of ultrastructure .
29321567	5	38	theme	ultrastructure	899:912	arg1	density					874:880	grafting density	865:880	grafting density	865:880	We analyse the relationship between the mechanical properties of the EG layer and several molecular parameters, including the filament bending rigidity, grafting density, and the type of ultrastructure .
29321567	5	38	theme	ultrastructure	899:912	arg1	type					891:894	and the type	883:894	and the type	883:894	We analyse the relationship between the mechanical properties of the EG layer and several molecular parameters, including the filament bending rigidity, grafting density, and the type of ultrastructure .
29321567	6	39	theme	small	1001:1005	arg1	deformations					1007:1018	small deformations	1001:1018	small deformations	1001:1018	We show that variations in the glycan density determine the elasticity of the EG for small deformations, and that the normal stress may be effectively dampened by the EG layer, preventing the stress from being transferred to the cell membrane.
29321567	1	40	theme	sugar-rich	121:130	arg1	layer					132:136	a sugar-rich layer	119:136	a sugar-rich layer that lines the luminal surface of blood vessels	119:184	The endothelial glycocalyx (EG), a sugar-rich layer that lines the luminal surface of blood vessels, is an important constituent of the vascular system.
29321567	1	40	theme	sugar-rich	121:130	arg1	glycocalyx					102:111	endothelial glycocalyx	90:111	The endothelial glycocalyx (EG)	86:116	The endothelial glycocalyx (EG), a sugar-rich layer that lines the luminal surface of blood vessels, is an important constituent of the vascular system.
29321567	5	41	theme	bending	847:853	arg1	rigidity					855:862	the filament bending rigidity	834:862	the filament bending rigidity	834:862	We analyse the relationship between the mechanical properties of the EG layer and several molecular parameters, including the filament bending rigidity, grafting density, and the type of ultrastructure .
29321567	5	41	theme	bending	847:853	arg1	density					874:880	grafting density	865:880	grafting density	865:880	We analyse the relationship between the mechanical properties of the EG layer and several molecular parameters, including the filament bending rigidity, grafting density, and the type of ultrastructure .
29321567	5	41	theme	bending	847:853	arg1	type					891:894	and the type	883:894	and the type	883:894	We analyse the relationship between the mechanical properties of the EG layer and several molecular parameters, including the filament bending rigidity, grafting density, and the type of ultrastructure .
29321567	7	42	theme	mechanical	1264:1273	arg1	resistance					1275:1284	the mechanical resistance	1260:1284	the mechanical resistance of the EG	1260:1294	Furthermore, our bush-like model allows us to evaluate the forces and energies required to overcome the mechanical resistance of the EG.
29321567	5	43	theme	layer	784:788	arg1	rigidity					855:862	the filament bending rigidity	834:862	the filament bending rigidity	834:862	We analyse the relationship between the mechanical properties of the EG layer and several molecular parameters, including the filament bending rigidity, grafting density, and the type of ultrastructure .
29321567	5	43	theme	layer	784:788	arg1	parameters					812:821	several molecular parameters	794:821	several molecular parameters	794:821	We analyse the relationship between the mechanical properties of the EG layer and several molecular parameters, including the filament bending rigidity, grafting density, and the type of ultrastructure .
29321567	5	43	theme	layer	784:788	arg1	properties					763:772	the mechanical properties	748:772	the mechanical properties of the EG layer	748:788	We analyse the relationship between the mechanical properties of the EG layer and several molecular parameters, including the filament bending rigidity, grafting density, and the type of ultrastructure .
29321567	5	44	theme	filament	838:845	arg1	rigidity					855:862	the filament bending rigidity	834:862	the filament bending rigidity	834:862	We analyse the relationship between the mechanical properties of the EG layer and several molecular parameters, including the filament bending rigidity, grafting density, and the type of ultrastructure .
29321567	5	44	theme	filament	838:845	arg1	density					874:880	grafting density	865:880	grafting density	865:880	We analyse the relationship between the mechanical properties of the EG layer and several molecular parameters, including the filament bending rigidity, grafting density, and the type of ultrastructure .
29321567	5	44	theme	filament	838:845	arg1	type					891:894	and the type	883:894	and the type	883:894	We analyse the relationship between the mechanical properties of the EG layer and several molecular parameters, including the filament bending rigidity, grafting density, and the type of ultrastructure .
29321567	1	45	theme	important	193:201	arg1	constituent					203:213	an important constituent	190:213	an important constituent of the vascular system	190:236	The endothelial glycocalyx (EG), a sugar-rich layer that lines the luminal surface of blood vessels, is an important constituent of the vascular system.
29321567	1	45	theme	important	193:201	arg1	glycocalyx					102:111	endothelial glycocalyx	90:111	The endothelial glycocalyx (EG)	86:116	The endothelial glycocalyx (EG), a sugar-rich layer that lines the luminal surface of blood vessels, is an important constituent of the vascular system.
29321567	3	46	theme	sufficient	464:473	arg1	insight					475:481	sufficient insight	464:481	sufficient insight into its molecular organisation	464:513	While previous experiments probed the properties of the layer at the continuum level, they did not provide sufficient insight into its molecular organisation.
29778448	0	0	theme	capillary	96:104	arg1	analysis					140:147	capillary electrophoresis-mass spectrometry analysis	96:147	capillary electrophoresis-mass spectrometry analysis	96:147	Multi-charged labeling of oligosaccharides and N-linked glycans by hexahistidine-based tags for capillary electrophoresis-mass spectrometry analysis.
29778448	2	1	from	effect	468:473	arg1	analysis					530:537	CE-MS analysis	524:537	CE-MS analysis	524:537	Peptide tags with a various number of histidine residues were tested for maltooligosaccharide labeling in order to investigate the effect of the size of labels and a number of charges on CE-MS analysis.
29778448	4	2	attach	released	1272:1279	arg1	fetuin					1293:1298	bovine fetuin	1286:1298	bovine fetuin	1286:1298	This labeling approach significantly improved sensitivity with LOD of labeled maltopentaose determined to be 40 nmol/L and also significantly reduced separation time of neutral maltooligosaccharides and N-linked glycans released from bovine ribonuclease B. Furthermore, the labeling by this multi-cationic peptide hydrazine tag also allowed performing analysis of acidic glycans by CE-MS in a positive ion mode as demonstrated by separation of sialylated N-linked glycans released from bovine fetuin.
29778448	4	2	attach	released	1272:1279	arg2	glycans					1264:1270	sialylated N-linked glycans	1244:1270	sialylated N-linked glycans released from bovine fetuin	1244:1298	This labeling approach significantly improved sensitivity with LOD of labeled maltopentaose determined to be 40 nmol/L and also significantly reduced separation time of neutral maltooligosaccharides and N-linked glycans released from bovine ribonuclease B. Furthermore, the labeling by this multi-cationic peptide hydrazine tag also allowed performing analysis of acidic glycans by CE-MS in a positive ion mode as demonstrated by separation of sialylated N-linked glycans released from bovine fetuin.
29778448	1	3	theme	capillary	284:292	arg1	CE-MS					329:333	CE-MS	329:333	CE-MS	329:333	The labeling by amino acids and peptides was investigated for sensitive and fast analyses of oligosaccharides and N-linked glycans by capillary electrophoresis-mass spectrometry (CE-MS).
29778448	1	3	theme	capillary	284:292	arg1	spectrometry					315:326	capillary electrophoresis-mass spectrometry	284:326	capillary electrophoresis-mass spectrometry (CE-MS)	284:334	The labeling by amino acids and peptides was investigated for sensitive and fast analyses of oligosaccharides and N-linked glycans by capillary electrophoresis-mass spectrometry (CE-MS).
29778448	2	4	theme	charges	513:519	arg1	number					503:508	a number	501:508	a number of charges	501:519	Peptide tags with a various number of histidine residues were tested for maltooligosaccharide labeling in order to investigate the effect of the size of labels and a number of charges on CE-MS analysis.
29778448	2	4	theme	charges	513:519	arg1	size					482:485	the size	478:485	the size of labels	478:495	Peptide tags with a various number of histidine residues were tested for maltooligosaccharide labeling in order to investigate the effect of the size of labels and a number of charges on CE-MS analysis.
29778448	3	5	theme	hydrazine	713:721	arg1	functionality					723:735	hydrazine functionality	713:735	hydrazine functionality	713:735	Nevertheless, the reductive amination labeling of N-linked glycans by a hexahistidine tag resulted in a multiple products formation, therefore a peptide tag was modified by hydrazine functionality in order to perform labeling by hydrazone formation chemistry.
29778448	1	6	theme	electrophoresis-mass	294:313	arg1	CE-MS					329:333	CE-MS	329:333	CE-MS	329:333	The labeling by amino acids and peptides was investigated for sensitive and fast analyses of oligosaccharides and N-linked glycans by capillary electrophoresis-mass spectrometry (CE-MS).
29778448	1	6	theme	electrophoresis-mass	294:313	arg1	spectrometry					315:326	capillary electrophoresis-mass spectrometry	284:326	capillary electrophoresis-mass spectrometry (CE-MS)	284:334	The labeling by amino acids and peptides was investigated for sensitive and fast analyses of oligosaccharides and N-linked glycans by capillary electrophoresis-mass spectrometry (CE-MS).
29778448	0	7	theme	spectrometry	127:138	arg1	analysis					140:147	capillary electrophoresis-mass spectrometry analysis	96:147	capillary electrophoresis-mass spectrometry analysis	96:147	Multi-charged labeling of oligosaccharides and N-linked glycans by hexahistidine-based tags for capillary electrophoresis-mass spectrometry analysis.
29778448	3	8	mod	modified	701:708	arg3	functionality					723:735	hydrazine functionality	713:735	hydrazine functionality	713:735	Nevertheless, the reductive amination labeling of N-linked glycans by a hexahistidine tag resulted in a multiple products formation, therefore a peptide tag was modified by hydrazine functionality in order to perform labeling by hydrazone formation chemistry.
29778448	3	8	mod	modified	701:708	arg1	tag					693:695	a peptide tag	683:695	a peptide tag	683:695	Nevertheless, the reductive amination labeling of N-linked glycans by a hexahistidine tag resulted in a multiple products formation, therefore a peptide tag was modified by hydrazine functionality in order to perform labeling by hydrazone formation chemistry.
29778448	2	9	theme	Peptide	337:343	arg1	tags					345:348	Peptide tags	337:348	Peptide tags with a various number of histidine residues	337:392	Peptide tags with a various number of histidine residues were tested for maltooligosaccharide labeling in order to investigate the effect of the size of labels and a number of charges on CE-MS analysis.
29778448	4	10	theme	sialylated	1244:1253	arg1	glycans					1264:1270	sialylated N-linked glycans	1244:1270	sialylated N-linked glycans released from bovine fetuin	1244:1298	This labeling approach significantly improved sensitivity with LOD of labeled maltopentaose determined to be 40 nmol/L and also significantly reduced separation time of neutral maltooligosaccharides and N-linked glycans released from bovine ribonuclease B. Furthermore, the labeling by this multi-cationic peptide hydrazine tag also allowed performing analysis of acidic glycans by CE-MS in a positive ion mode as demonstrated by separation of sialylated N-linked glycans released from bovine fetuin.
29778448	0	11	theme	electrophoresis-mass	106:125	arg1	analysis					140:147	capillary electrophoresis-mass spectrometry analysis	96:147	capillary electrophoresis-mass spectrometry analysis	96:147	Multi-charged labeling of oligosaccharides and N-linked glycans by hexahistidine-based tags for capillary electrophoresis-mass spectrometry analysis.
29778448	4	12	from	CE-MS	1182:1186	arg1	mode					1206:1209	a positive ion mode	1191:1209	a positive ion mode	1191:1209	This labeling approach significantly improved sensitivity with LOD of labeled maltopentaose determined to be 40 nmol/L and also significantly reduced separation time of neutral maltooligosaccharides and N-linked glycans released from bovine ribonuclease B. Furthermore, the labeling by this multi-cationic peptide hydrazine tag also allowed performing analysis of acidic glycans by CE-MS in a positive ion mode as demonstrated by separation of sialylated N-linked glycans released from bovine fetuin.
29778448	3	13	theme	reductive	558:566	arg1	amination					568:576	the reductive amination	554:576	the reductive amination labeling of N-linked glycans by a hexahistidine tag	554:628	Nevertheless, the reductive amination labeling of N-linked glycans by a hexahistidine tag resulted in a multiple products formation, therefore a peptide tag was modified by hydrazine functionality in order to perform labeling by hydrazone formation chemistry.
29778448	4	14	theme	bovine	1034:1039	arg1	ribonuclease					1041:1052	bovine ribonuclease	1034:1052	bovine ribonuclease B. Furthermore, the labeling by this multi-cationic peptide hydrazine tag also allowed performing analysis of acidic glycans by CE-MS in a positive ion mode as demonstrated by separation of sialylated N-linked glycans released from bovine fetuin	1034:1298	This labeling approach significantly improved sensitivity with LOD of labeled maltopentaose determined to be 40 nmol/L and also significantly reduced separation time of neutral maltooligosaccharides and N-linked glycans released from bovine ribonuclease B. Furthermore, the labeling by this multi-cationic peptide hydrazine tag also allowed performing analysis of acidic glycans by CE-MS in a positive ion mode as demonstrated by separation of sialylated N-linked glycans released from bovine fetuin.
29778448	3	15	theme	amination	568:576	arg1	labeling					578:585	the reductive amination labeling	554:585	the reductive amination labeling of N-linked glycans by a hexahistidine tag	554:628	Nevertheless, the reductive amination labeling of N-linked glycans by a hexahistidine tag resulted in a multiple products formation, therefore a peptide tag was modified by hydrazine functionality in order to perform labeling by hydrazone formation chemistry.
29778448	2	16	theme	labels	490:495	arg1	number					503:508	a number	501:508	a number of charges	501:519	Peptide tags with a various number of histidine residues were tested for maltooligosaccharide labeling in order to investigate the effect of the size of labels and a number of charges on CE-MS analysis.
29778448	2	16	theme	labels	490:495	arg1	size					482:485	the size	478:485	the size of labels	478:495	Peptide tags with a various number of histidine residues were tested for maltooligosaccharide labeling in order to investigate the effect of the size of labels and a number of charges on CE-MS analysis.
29778448	2	17	theme	various	357:363	arg1	number					365:370	a various number	355:370	a various number of histidine residues	355:392	Peptide tags with a various number of histidine residues were tested for maltooligosaccharide labeling in order to investigate the effect of the size of labels and a number of charges on CE-MS analysis.
29778448	1	18	theme	sensitive	212:220	arg1	analyses					231:238	sensitive and fast analyses	212:238	sensitive and fast analyses of oligosaccharides and N-linked glycans	212:279	The labeling by amino acids and peptides was investigated for sensitive and fast analyses of oligosaccharides and N-linked glycans by capillary electrophoresis-mass spectrometry (CE-MS).
29778448	0	19	theme	Multi-charged	0:12	arg1	labeling					14:21	Multi-charged labeling	0:21	Multi-charged labeling of oligosaccharides and N-linked glycans by hexahistidine-based tags for capillary electrophoresis-mass spectrometry analysis.	0:148	Multi-charged labeling of oligosaccharides and N-linked glycans by hexahistidine-based tags for capillary electrophoresis-mass spectrometry analysis.
29778448	0	20	link	N-linked	47:54	arg1	glycans					56:62	N-linked glycans	47:62	N-linked glycans	47:62	Multi-charged labeling of oligosaccharides and N-linked glycans by hexahistidine-based tags for capillary electrophoresis-mass spectrometry analysis.
29778448	4	21	theme	peptide	1106:1112	arg1	tag					1124:1126	this multi-cationic peptide hydrazine tag	1086:1126	this multi-cationic peptide hydrazine tag	1086:1126	This labeling approach significantly improved sensitivity with LOD of labeled maltopentaose determined to be 40 nmol/L and also significantly reduced separation time of neutral maltooligosaccharides and N-linked glycans released from bovine ribonuclease B. Furthermore, the labeling by this multi-cationic peptide hydrazine tag also allowed performing analysis of acidic glycans by CE-MS in a positive ion mode as demonstrated by separation of sialylated N-linked glycans released from bovine fetuin.
29778448	4	22	link	N-linked	1255:1262	arg1	glycans					1264:1270	sialylated N-linked glycans	1244:1270	sialylated N-linked glycans released from bovine fetuin	1244:1298	This labeling approach significantly improved sensitivity with LOD of labeled maltopentaose determined to be 40 nmol/L and also significantly reduced separation time of neutral maltooligosaccharides and N-linked glycans released from bovine ribonuclease B. Furthermore, the labeling by this multi-cationic peptide hydrazine tag also allowed performing analysis of acidic glycans by CE-MS in a positive ion mode as demonstrated by separation of sialylated N-linked glycans released from bovine fetuin.
29778448	4	23	dep	improved	837:844	arg1	reduced					942:948	reduced	942:948	reduced separation time of neutral maltooligosaccharides and N-linked glycans released from bovine ribonuclease B. Furthermore, the labeling by this multi-cationic peptide hydrazine tag also allowed performing analysis of acidic glycans by CE-MS in a positive ion mode as demonstrated by separation of sialylated N-linked glycans released from bovine fetuin	942:1298	This labeling approach significantly improved sensitivity with LOD of labeled maltopentaose determined to be 40 nmol/L and also significantly reduced separation time of neutral maltooligosaccharides and N-linked glycans released from bovine ribonuclease B. Furthermore, the labeling by this multi-cationic peptide hydrazine tag also allowed performing analysis of acidic glycans by CE-MS in a positive ion mode as demonstrated by separation of sialylated N-linked glycans released from bovine fetuin.
29778448	0	24	theme	oligosaccharides	26:41	arg1	labeling					14:21	Multi-charged labeling	0:21	Multi-charged labeling of oligosaccharides and N-linked glycans by hexahistidine-based tags for capillary electrophoresis-mass spectrometry analysis.	0:148	Multi-charged labeling of oligosaccharides and N-linked glycans by hexahistidine-based tags for capillary electrophoresis-mass spectrometry analysis.
29778448	4	25	dep	ribonuclease	1041:1052	arg1	allowed					1133:1139	allowed	1133:1139	allowed performing analysis of acidic glycans by CE-MS in a positive ion mode as demonstrated by separation of sialylated N-linked glycans released from bovine fetuin	1133:1298	This labeling approach significantly improved sensitivity with LOD of labeled maltopentaose determined to be 40 nmol/L and also significantly reduced separation time of neutral maltooligosaccharides and N-linked glycans released from bovine ribonuclease B. Furthermore, the labeling by this multi-cationic peptide hydrazine tag also allowed performing analysis of acidic glycans by CE-MS in a positive ion mode as demonstrated by separation of sialylated N-linked glycans released from bovine fetuin.
29778448	1	26	link	N-linked	264:271	arg1	glycans					273:279	N-linked glycans	264:279	N-linked glycans	264:279	The labeling by amino acids and peptides was investigated for sensitive and fast analyses of oligosaccharides and N-linked glycans by capillary electrophoresis-mass spectrometry (CE-MS).
29778448	3	27	theme	N-linked	590:597	arg1	glycans					599:605	N-linked glycans	590:605	N-linked glycans	590:605	Nevertheless, the reductive amination labeling of N-linked glycans by a hexahistidine tag resulted in a multiple products formation, therefore a peptide tag was modified by hydrazine functionality in order to perform labeling by hydrazone formation chemistry.
29778448	1	28	theme	fast	226:229	arg1	analyses					231:238	sensitive and fast analyses	212:238	sensitive and fast analyses of oligosaccharides and N-linked glycans	212:279	The labeling by amino acids and peptides was investigated for sensitive and fast analyses of oligosaccharides and N-linked glycans by capillary electrophoresis-mass spectrometry (CE-MS).
29778448	3	29	theme	hydrazone	769:777	arg1	chemistry					789:797	hydrazone formation chemistry	769:797	hydrazone formation chemistry	769:797	Nevertheless, the reductive amination labeling of N-linked glycans by a hexahistidine tag resulted in a multiple products formation, therefore a peptide tag was modified by hydrazine functionality in order to perform labeling by hydrazone formation chemistry.
29778448	4	30	theme	maltooligosaccharides	977:997	arg1	time					961:964	separation time	950:964	separation time of neutral maltooligosaccharides and N-linked glycans released from bovine ribonuclease B. Furthermore, the labeling by this multi-cationic peptide hydrazine tag also allowed performing analysis of acidic glycans by CE-MS in a positive ion mode as demonstrated by separation of sialylated N-linked glycans released from bovine fetuin	950:1298	This labeling approach significantly improved sensitivity with LOD of labeled maltopentaose determined to be 40 nmol/L and also significantly reduced separation time of neutral maltooligosaccharides and N-linked glycans released from bovine ribonuclease B. Furthermore, the labeling by this multi-cationic peptide hydrazine tag also allowed performing analysis of acidic glycans by CE-MS in a positive ion mode as demonstrated by separation of sialylated N-linked glycans released from bovine fetuin.
29778448	4	31	theme	N-linked	1003:1010	arg1	glycans					1012:1018	N-linked glycans	1003:1018	N-linked glycans	1003:1018	This labeling approach significantly improved sensitivity with LOD of labeled maltopentaose determined to be 40 nmol/L and also significantly reduced separation time of neutral maltooligosaccharides and N-linked glycans released from bovine ribonuclease B. Furthermore, the labeling by this multi-cationic peptide hydrazine tag also allowed performing analysis of acidic glycans by CE-MS in a positive ion mode as demonstrated by separation of sialylated N-linked glycans released from bovine fetuin.
29778448	3	32	theme	glycans	599:605	arg1	labeling					578:585	the reductive amination labeling	554:585	the reductive amination labeling of N-linked glycans by a hexahistidine tag	554:628	Nevertheless, the reductive amination labeling of N-linked glycans by a hexahistidine tag resulted in a multiple products formation, therefore a peptide tag was modified by hydrazine functionality in order to perform labeling by hydrazone formation chemistry.
29778448	4	33	theme	ion	1202:1204	arg1	mode					1206:1209	a positive ion mode	1191:1209	a positive ion mode	1191:1209	This labeling approach significantly improved sensitivity with LOD of labeled maltopentaose determined to be 40 nmol/L and also significantly reduced separation time of neutral maltooligosaccharides and N-linked glycans released from bovine ribonuclease B. Furthermore, the labeling by this multi-cationic peptide hydrazine tag also allowed performing analysis of acidic glycans by CE-MS in a positive ion mode as demonstrated by separation of sialylated N-linked glycans released from bovine fetuin.
29778448	0	34	theme	N-linked	47:54	arg1	glycans					56:62	N-linked glycans	47:62	N-linked glycans	47:62	Multi-charged labeling of oligosaccharides and N-linked glycans by hexahistidine-based tags for capillary electrophoresis-mass spectrometry analysis.
29778448	3	35	theme	formation	779:787	arg1	chemistry					789:797	hydrazone formation chemistry	769:797	hydrazone formation chemistry	769:797	Nevertheless, the reductive amination labeling of N-linked glycans by a hexahistidine tag resulted in a multiple products formation, therefore a peptide tag was modified by hydrazine functionality in order to perform labeling by hydrazone formation chemistry.
29778448	4	36	theme	glycans	1012:1018	arg1	time					961:964	separation time	950:964	separation time of neutral maltooligosaccharides and N-linked glycans released from bovine ribonuclease B. Furthermore, the labeling by this multi-cationic peptide hydrazine tag also allowed performing analysis of acidic glycans by CE-MS in a positive ion mode as demonstrated by separation of sialylated N-linked glycans released from bovine fetuin	950:1298	This labeling approach significantly improved sensitivity with LOD of labeled maltopentaose determined to be 40 nmol/L and also significantly reduced separation time of neutral maltooligosaccharides and N-linked glycans released from bovine ribonuclease B. Furthermore, the labeling by this multi-cationic peptide hydrazine tag also allowed performing analysis of acidic glycans by CE-MS in a positive ion mode as demonstrated by separation of sialylated N-linked glycans released from bovine fetuin.
29778448	3	37	theme	peptide	685:691	arg1	tag					693:695	a peptide tag	683:695	a peptide tag	683:695	Nevertheless, the reductive amination labeling of N-linked glycans by a hexahistidine tag resulted in a multiple products formation, therefore a peptide tag was modified by hydrazine functionality in order to perform labeling by hydrazone formation chemistry.
29778448	4	38	theme	labeled	870:876	arg1	maltopentaose					878:890	labeled maltopentaose	870:890	labeled maltopentaose determined to be 40 nmol/L	870:917	This labeling approach significantly improved sensitivity with LOD of labeled maltopentaose determined to be 40 nmol/L and also significantly reduced separation time of neutral maltooligosaccharides and N-linked glycans released from bovine ribonuclease B. Furthermore, the labeling by this multi-cationic peptide hydrazine tag also allowed performing analysis of acidic glycans by CE-MS in a positive ion mode as demonstrated by separation of sialylated N-linked glycans released from bovine fetuin.
29778448	4	39	theme	positive	1193:1200	arg1	mode					1206:1209	a positive ion mode	1191:1209	a positive ion mode	1191:1209	This labeling approach significantly improved sensitivity with LOD of labeled maltopentaose determined to be 40 nmol/L and also significantly reduced separation time of neutral maltooligosaccharides and N-linked glycans released from bovine ribonuclease B. Furthermore, the labeling by this multi-cationic peptide hydrazine tag also allowed performing analysis of acidic glycans by CE-MS in a positive ion mode as demonstrated by separation of sialylated N-linked glycans released from bovine fetuin.
29778448	2	40	theme	number	503:508	arg1	effect					468:473	the effect	464:473	the effect of the size of labels and a number of charges on CE-MS analysis	464:537	Peptide tags with a various number of histidine residues were tested for maltooligosaccharide labeling in order to investigate the effect of the size of labels and a number of charges on CE-MS analysis.
29778448	2	41	with	tags	345:348	arg1	number					365:370	a various number	355:370	a various number of histidine residues	355:392	Peptide tags with a various number of histidine residues were tested for maltooligosaccharide labeling in order to investigate the effect of the size of labels and a number of charges on CE-MS analysis.
29778448	2	42	theme	size	482:485	arg1	effect					468:473	the effect	464:473	the effect of the size of labels and a number of charges on CE-MS analysis	464:537	Peptide tags with a various number of histidine residues were tested for maltooligosaccharide labeling in order to investigate the effect of the size of labels and a number of charges on CE-MS analysis.
29778448	1	43	theme	oligosaccharides	243:258	arg1	analyses					231:238	sensitive and fast analyses	212:238	sensitive and fast analyses of oligosaccharides and N-linked glycans	212:279	The labeling by amino acids and peptides was investigated for sensitive and fast analyses of oligosaccharides and N-linked glycans by capillary electrophoresis-mass spectrometry (CE-MS).
29778448	4	44	theme	multi-cationic	1091:1104	arg1	tag					1124:1126	this multi-cationic peptide hydrazine tag	1086:1126	this multi-cationic peptide hydrazine tag	1086:1126	This labeling approach significantly improved sensitivity with LOD of labeled maltopentaose determined to be 40 nmol/L and also significantly reduced separation time of neutral maltooligosaccharides and N-linked glycans released from bovine ribonuclease B. Furthermore, the labeling by this multi-cationic peptide hydrazine tag also allowed performing analysis of acidic glycans by CE-MS in a positive ion mode as demonstrated by separation of sialylated N-linked glycans released from bovine fetuin.
29778448	0	45	theme	hexahistidine-based	67:85	arg1	tags					87:90	hexahistidine-based tags	67:90	hexahistidine-based tags for capillary electrophoresis-mass spectrometry analysis	67:147	Multi-charged labeling of oligosaccharides and N-linked glycans by hexahistidine-based tags for capillary electrophoresis-mass spectrometry analysis.
29778448	3	46	theme	hexahistidine	612:624	arg1	tag					626:628	a hexahistidine tag	610:628	a hexahistidine tag	610:628	Nevertheless, the reductive amination labeling of N-linked glycans by a hexahistidine tag resulted in a multiple products formation, therefore a peptide tag was modified by hydrazine functionality in order to perform labeling by hydrazone formation chemistry.
29778448	4	47	theme	glycans	1264:1270	arg1	separation					1230:1239	separation	1230:1239	separation of sialylated N-linked glycans released from bovine fetuin	1230:1298	This labeling approach significantly improved sensitivity with LOD of labeled maltopentaose determined to be 40 nmol/L and also significantly reduced separation time of neutral maltooligosaccharides and N-linked glycans released from bovine ribonuclease B. Furthermore, the labeling by this multi-cationic peptide hydrazine tag also allowed performing analysis of acidic glycans by CE-MS in a positive ion mode as demonstrated by separation of sialylated N-linked glycans released from bovine fetuin.
29778448	2	48	theme	residues	385:392	arg1	number					365:370	a various number	355:370	a various number of histidine residues	355:392	Peptide tags with a various number of histidine residues were tested for maltooligosaccharide labeling in order to investigate the effect of the size of labels and a number of charges on CE-MS analysis.
29778448	0	49	theme	glycans	56:62	arg1	labeling					14:21	Multi-charged labeling	0:21	Multi-charged labeling of oligosaccharides and N-linked glycans by hexahistidine-based tags for capillary electrophoresis-mass spectrometry analysis.	0:148	Multi-charged labeling of oligosaccharides and N-linked glycans by hexahistidine-based tags for capillary electrophoresis-mass spectrometry analysis.
29778448	4	50	gly	sialylated	1244:1253	arg1	glycans					1264:1270	sialylated N-linked glycans	1244:1270	sialylated N-linked glycans released from bovine fetuin	1244:1298	This labeling approach significantly improved sensitivity with LOD of labeled maltopentaose determined to be 40 nmol/L and also significantly reduced separation time of neutral maltooligosaccharides and N-linked glycans released from bovine ribonuclease B. Furthermore, the labeling by this multi-cationic peptide hydrazine tag also allowed performing analysis of acidic glycans by CE-MS in a positive ion mode as demonstrated by separation of sialylated N-linked glycans released from bovine fetuin.
29778448	4	51	theme	separation	950:959	arg1	time					961:964	separation time	950:964	separation time of neutral maltooligosaccharides and N-linked glycans released from bovine ribonuclease B. Furthermore, the labeling by this multi-cationic peptide hydrazine tag also allowed performing analysis of acidic glycans by CE-MS in a positive ion mode as demonstrated by separation of sialylated N-linked glycans released from bovine fetuin	950:1298	This labeling approach significantly improved sensitivity with LOD of labeled maltopentaose determined to be 40 nmol/L and also significantly reduced separation time of neutral maltooligosaccharides and N-linked glycans released from bovine ribonuclease B. Furthermore, the labeling by this multi-cationic peptide hydrazine tag also allowed performing analysis of acidic glycans by CE-MS in a positive ion mode as demonstrated by separation of sialylated N-linked glycans released from bovine fetuin.
29778448	4	52	theme	maltopentaose	878:890	arg1	LOD					863:865	LOD	863:865	LOD of labeled maltopentaose determined to be 40 nmol/L	863:917	This labeling approach significantly improved sensitivity with LOD of labeled maltopentaose determined to be 40 nmol/L and also significantly reduced separation time of neutral maltooligosaccharides and N-linked glycans released from bovine ribonuclease B. Furthermore, the labeling by this multi-cationic peptide hydrazine tag also allowed performing analysis of acidic glycans by CE-MS in a positive ion mode as demonstrated by separation of sialylated N-linked glycans released from bovine fetuin.
29778448	4	53	theme	bovine	1286:1291	arg1	fetuin					1293:1298	bovine fetuin	1286:1298	bovine fetuin	1286:1298	This labeling approach significantly improved sensitivity with LOD of labeled maltopentaose determined to be 40 nmol/L and also significantly reduced separation time of neutral maltooligosaccharides and N-linked glycans released from bovine ribonuclease B. Furthermore, the labeling by this multi-cationic peptide hydrazine tag also allowed performing analysis of acidic glycans by CE-MS in a positive ion mode as demonstrated by separation of sialylated N-linked glycans released from bovine fetuin.
29778448	2	54	theme	histidine	375:383	arg1	residues					385:392	histidine residues	375:392	histidine residues	375:392	Peptide tags with a various number of histidine residues were tested for maltooligosaccharide labeling in order to investigate the effect of the size of labels and a number of charges on CE-MS analysis.
29778448	4	55	theme	glycans	1171:1177	arg1	analysis					1152:1159	performing analysis	1141:1159	performing analysis of acidic glycans	1141:1177	This labeling approach significantly improved sensitivity with LOD of labeled maltopentaose determined to be 40 nmol/L and also significantly reduced separation time of neutral maltooligosaccharides and N-linked glycans released from bovine ribonuclease B. Furthermore, the labeling by this multi-cationic peptide hydrazine tag also allowed performing analysis of acidic glycans by CE-MS in a positive ion mode as demonstrated by separation of sialylated N-linked glycans released from bovine fetuin.
29778448	4	56	theme	neutral	969:975	arg1	maltooligosaccharides					977:997	neutral maltooligosaccharides	969:997	neutral maltooligosaccharides	969:997	This labeling approach significantly improved sensitivity with LOD of labeled maltopentaose determined to be 40 nmol/L and also significantly reduced separation time of neutral maltooligosaccharides and N-linked glycans released from bovine ribonuclease B. Furthermore, the labeling by this multi-cationic peptide hydrazine tag also allowed performing analysis of acidic glycans by CE-MS in a positive ion mode as demonstrated by separation of sialylated N-linked glycans released from bovine fetuin.
29778448	3	57	theme	products	653:660	arg1	formation					662:670	a multiple products formation	642:670	a multiple products formation	642:670	Nevertheless, the reductive amination labeling of N-linked glycans by a hexahistidine tag resulted in a multiple products formation, therefore a peptide tag was modified by hydrazine functionality in order to perform labeling by hydrazone formation chemistry.
29778448	4	58	theme	acidic	1164:1169	arg1	glycans					1171:1177	acidic glycans	1164:1177	acidic glycans	1164:1177	This labeling approach significantly improved sensitivity with LOD of labeled maltopentaose determined to be 40 nmol/L and also significantly reduced separation time of neutral maltooligosaccharides and N-linked glycans released from bovine ribonuclease B. Furthermore, the labeling by this multi-cationic peptide hydrazine tag also allowed performing analysis of acidic glycans by CE-MS in a positive ion mode as demonstrated by separation of sialylated N-linked glycans released from bovine fetuin.
29778448	4	59	link	N-linked	1003:1010	arg1	glycans					1012:1018	N-linked glycans	1003:1018	N-linked glycans	1003:1018	This labeling approach significantly improved sensitivity with LOD of labeled maltopentaose determined to be 40 nmol/L and also significantly reduced separation time of neutral maltooligosaccharides and N-linked glycans released from bovine ribonuclease B. Furthermore, the labeling by this multi-cationic peptide hydrazine tag also allowed performing analysis of acidic glycans by CE-MS in a positive ion mode as demonstrated by separation of sialylated N-linked glycans released from bovine fetuin.
29778448	4	60	theme	labeling	805:812	arg1	approach					814:821	This labeling approach	800:821	This labeling approach	800:821	This labeling approach significantly improved sensitivity with LOD of labeled maltopentaose determined to be 40 nmol/L and also significantly reduced separation time of neutral maltooligosaccharides and N-linked glycans released from bovine ribonuclease B. Furthermore, the labeling by this multi-cationic peptide hydrazine tag also allowed performing analysis of acidic glycans by CE-MS in a positive ion mode as demonstrated by separation of sialylated N-linked glycans released from bovine fetuin.
29778448	4	61	attach	released	1020:1027	arg1	ribonuclease					1041:1052	bovine ribonuclease	1034:1052	bovine ribonuclease B. Furthermore, the labeling by this multi-cationic peptide hydrazine tag also allowed performing analysis of acidic glycans by CE-MS in a positive ion mode as demonstrated by separation of sialylated N-linked glycans released from bovine fetuin	1034:1298	This labeling approach significantly improved sensitivity with LOD of labeled maltopentaose determined to be 40 nmol/L and also significantly reduced separation time of neutral maltooligosaccharides and N-linked glycans released from bovine ribonuclease B. Furthermore, the labeling by this multi-cationic peptide hydrazine tag also allowed performing analysis of acidic glycans by CE-MS in a positive ion mode as demonstrated by separation of sialylated N-linked glycans released from bovine fetuin.
29778448	4	61	attach	released	1020:1027	arg2	maltooligosaccharides					977:997	neutral maltooligosaccharides	969:997	neutral maltooligosaccharides	969:997	This labeling approach significantly improved sensitivity with LOD of labeled maltopentaose determined to be 40 nmol/L and also significantly reduced separation time of neutral maltooligosaccharides and N-linked glycans released from bovine ribonuclease B. Furthermore, the labeling by this multi-cationic peptide hydrazine tag also allowed performing analysis of acidic glycans by CE-MS in a positive ion mode as demonstrated by separation of sialylated N-linked glycans released from bovine fetuin.
29778448	1	62	theme	amino	166:170	arg1	acids					172:176	amino acids	166:176	amino acids	166:176	The labeling by amino acids and peptides was investigated for sensitive and fast analyses of oligosaccharides and N-linked glycans by capillary electrophoresis-mass spectrometry (CE-MS).
29778448	1	63	theme	N-linked	264:271	arg1	glycans					273:279	N-linked glycans	264:279	N-linked glycans	264:279	The labeling by amino acids and peptides was investigated for sensitive and fast analyses of oligosaccharides and N-linked glycans by capillary electrophoresis-mass spectrometry (CE-MS).
29778448	3	64	link	N-linked	590:597	arg1	glycans					599:605	N-linked glycans	590:605	N-linked glycans	590:605	Nevertheless, the reductive amination labeling of N-linked glycans by a hexahistidine tag resulted in a multiple products formation, therefore a peptide tag was modified by hydrazine functionality in order to perform labeling by hydrazone formation chemistry.
29778448	2	65	theme	maltooligosaccharide	410:429	arg1	labeling					431:438	maltooligosaccharide labeling	410:438	maltooligosaccharide labeling	410:438	Peptide tags with a various number of histidine residues were tested for maltooligosaccharide labeling in order to investigate the effect of the size of labels and a number of charges on CE-MS analysis.
29778448	1	66	theme	glycans	273:279	arg1	analyses					231:238	sensitive and fast analyses	212:238	sensitive and fast analyses of oligosaccharides and N-linked glycans	212:279	The labeling by amino acids and peptides was investigated for sensitive and fast analyses of oligosaccharides and N-linked glycans by capillary electrophoresis-mass spectrometry (CE-MS).
29778448	2	67	theme	CE-MS	524:528	arg1	analysis					530:537	CE-MS analysis	524:537	CE-MS analysis	524:537	Peptide tags with a various number of histidine residues were tested for maltooligosaccharide labeling in order to investigate the effect of the size of labels and a number of charges on CE-MS analysis.
29778448	3	68	theme	multiple	644:651	arg1	formation					662:670	a multiple products formation	642:670	a multiple products formation	642:670	Nevertheless, the reductive amination labeling of N-linked glycans by a hexahistidine tag resulted in a multiple products formation, therefore a peptide tag was modified by hydrazine functionality in order to perform labeling by hydrazone formation chemistry.
29778448	4	69	theme	N-linked	1255:1262	arg1	glycans					1264:1270	sialylated N-linked glycans	1244:1270	sialylated N-linked glycans released from bovine fetuin	1244:1298	This labeling approach significantly improved sensitivity with LOD of labeled maltopentaose determined to be 40 nmol/L and also significantly reduced separation time of neutral maltooligosaccharides and N-linked glycans released from bovine ribonuclease B. Furthermore, the labeling by this multi-cationic peptide hydrazine tag also allowed performing analysis of acidic glycans by CE-MS in a positive ion mode as demonstrated by separation of sialylated N-linked glycans released from bovine fetuin.
29778448	4	70	theme	hydrazine	1114:1122	arg1	tag					1124:1126	this multi-cationic peptide hydrazine tag	1086:1126	this multi-cationic peptide hydrazine tag	1086:1126	This labeling approach significantly improved sensitivity with LOD of labeled maltopentaose determined to be 40 nmol/L and also significantly reduced separation time of neutral maltooligosaccharides and N-linked glycans released from bovine ribonuclease B. Furthermore, the labeling by this multi-cationic peptide hydrazine tag also allowed performing analysis of acidic glycans by CE-MS in a positive ion mode as demonstrated by separation of sialylated N-linked glycans released from bovine fetuin.
29778448	4	71	theme	performing	1141:1150	arg1	analysis					1152:1159	performing analysis	1141:1159	performing analysis of acidic glycans	1141:1177	This labeling approach significantly improved sensitivity with LOD of labeled maltopentaose determined to be 40 nmol/L and also significantly reduced separation time of neutral maltooligosaccharides and N-linked glycans released from bovine ribonuclease B. Furthermore, the labeling by this multi-cationic peptide hydrazine tag also allowed performing analysis of acidic glycans by CE-MS in a positive ion mode as demonstrated by separation of sialylated N-linked glycans released from bovine fetuin.
31177828	0	0	theme	abiotic	72:78	arg1	surface					80:86	an abiotic surface	69:86	an abiotic surface	69:86	In situ spectroscopic analysis of Lactobacillus rhamnosus GG flow on an abiotic surface reveals a role for nutrients in biofilm development.
31177828	1	1	theme	biochemical	216:226	arg1	composition					228:238	the biochemical composition	212:238	the biochemical composition of biofilms of the probiotic bacterium Lactobacillus rhamnosus GG (LGG)	212:310	In this work, infrared spectroscopy was used to monitor the changes in the biochemical composition of biofilms of the probiotic bacterium Lactobacillus rhamnosus GG (LGG) in three nutritive media (10-fold diluted MRS, AOAC, and mTSB), in situ and under flow conditions.
31177828	2	2	theme	Epifluorescence	411:425	arg1	microscopy					427:436	Epifluorescence microscopy	411:436	Epifluorescence microscopy	411:436	Epifluorescence microscopy was used to observe the shape of LGG cells and their distribution on the surface.
31177828	3	3	theme	acids	625:629	arg1	content					606:612	a medium-dependent content	587:612	a medium-dependent content of nucleic acids, phospholipids and polysaccharides in the biofilms	587:680	Spectroscopic fingerprints recorded as a function of time revealed a medium-dependent content of nucleic acids, phospholipids and polysaccharides in the biofilms.
31177828	3	4	theme	medium-dependent	589:604	arg1	content					606:612	a medium-dependent content	587:612	a medium-dependent content of nucleic acids, phospholipids and polysaccharides in the biofilms	587:680	Spectroscopic fingerprints recorded as a function of time revealed a medium-dependent content of nucleic acids, phospholipids and polysaccharides in the biofilms.
31177828	6	5	theme	nutritional	996:1006	arg1	quality					1008:1014	the nutritional quality	992:1014	the nutritional quality of mTSB/10	992:1025	The rod shape of the cells was preserved in MRS/10, whereas acidic stress induced in AOAC/10 and the nutritional quality of mTSB/10 led to strong morphological changes.
31177828	6	6	theme	cells	916:920	arg1	shape					903:907	The rod shape	895:907	The rod shape of the cells	895:920	The rod shape of the cells was preserved in MRS/10, whereas acidic stress induced in AOAC/10 and the nutritional quality of mTSB/10 led to strong morphological changes.
31177828	3	7	from	content	606:612	arg1	biofilms					673:680	the biofilms	669:680	the biofilms	669:680	Spectroscopic fingerprints recorded as a function of time revealed a medium-dependent content of nucleic acids, phospholipids and polysaccharides in the biofilms.
31177828	4	8	located	observed	740:747	arg1	addition					686:693	addition	686:693	addition	686:693	In addition, time-dependent synthesis of lactic acid was observed in MRS/10 and AOAC/10.
31177828	4	8	located	observed	740:747	arg2	synthesis					711:719	time-dependent synthesis	696:719	time-dependent synthesis of lactic acid	696:734	In addition, time-dependent synthesis of lactic acid was observed in MRS/10 and AOAC/10.
31177828	4	8	located	observed	740:747	arg1	AOAC/10					763:769	AOAC/10	763:769	AOAC/10	763:769	In addition, time-dependent synthesis of lactic acid was observed in MRS/10 and AOAC/10.
31177828	4	8	located	observed	740:747	arg1	MRS/10					752:757	MRS/10	752:757	MRS/10	752:757	In addition, time-dependent synthesis of lactic acid was observed in MRS/10 and AOAC/10.
31177828	1	9	theme	nutritive	321:329	arg1	media					331:335	three nutritive media	315:335	three nutritive media (10-fold diluted MRS, AOAC, and mTSB)	315:373	In this work, infrared spectroscopy was used to monitor the changes in the biochemical composition of biofilms of the probiotic bacterium Lactobacillus rhamnosus GG (LGG) in three nutritive media (10-fold diluted MRS, AOAC, and mTSB), in situ and under flow conditions.
31177828	1	9	theme	nutritive	321:329	arg1	AOAC					359:362	AOAC	359:362	AOAC	359:362	In this work, infrared spectroscopy was used to monitor the changes in the biochemical composition of biofilms of the probiotic bacterium Lactobacillus rhamnosus GG (LGG) in three nutritive media (10-fold diluted MRS, AOAC, and mTSB), in situ and under flow conditions.
31177828	1	9	theme	nutritive	321:329	arg1	mTSB					369:372	mTSB	369:372	mTSB	369:372	In this work, infrared spectroscopy was used to monitor the changes in the biochemical composition of biofilms of the probiotic bacterium Lactobacillus rhamnosus GG (LGG) in three nutritive media (10-fold diluted MRS, AOAC, and mTSB), in situ and under flow conditions.
31177828	1	9	theme	nutritive	321:329	arg1	MRS					354:356	10-fold diluted MRS	338:356	10-fold diluted MRS	338:356	In this work, infrared spectroscopy was used to monitor the changes in the biochemical composition of biofilms of the probiotic bacterium Lactobacillus rhamnosus GG (LGG) in three nutritive media (10-fold diluted MRS, AOAC, and mTSB), in situ and under flow conditions.
31177828	4	10	theme	time-dependent	696:709	arg1	synthesis					711:719	time-dependent synthesis	696:719	time-dependent synthesis of lactic acid	696:734	In addition, time-dependent synthesis of lactic acid was observed in MRS/10 and AOAC/10.
31177828	1	11	theme	biofilms	243:250	arg1	composition					228:238	the biochemical composition	212:238	the biochemical composition of biofilms of the probiotic bacterium Lactobacillus rhamnosus GG (LGG)	212:310	In this work, infrared spectroscopy was used to monitor the changes in the biochemical composition of biofilms of the probiotic bacterium Lactobacillus rhamnosus GG (LGG) in three nutritive media (10-fold diluted MRS, AOAC, and mTSB), in situ and under flow conditions.
31177828	4	12	theme	lactic	724:729	arg1	acid					731:734	lactic acid	724:734	lactic acid	724:734	In addition, time-dependent synthesis of lactic acid was observed in MRS/10 and AOAC/10.
31177828	3	13	theme	polysaccharides	650:664	arg1	content					606:612	a medium-dependent content	587:612	a medium-dependent content of nucleic acids, phospholipids and polysaccharides in the biofilms	587:680	Spectroscopic fingerprints recorded as a function of time revealed a medium-dependent content of nucleic acids, phospholipids and polysaccharides in the biofilms.
31177828	2	14	from	distribution	491:502	arg1	surface					511:517	the surface	507:517	the surface	507:517	Epifluorescence microscopy was used to observe the shape of LGG cells and their distribution on the surface.
31177828	7	15	theme	nutritive	1093:1101	arg1	environment					1103:1113	the nutritive environment	1089:1113	the nutritive environment	1089:1113	These alterations due to the nutritive environment are important to consider in research and use of LGG biofilms.
31177828	5	16	theme	highest	809:815	arg1	extent					817:822	the highest extent	805:822	the highest extent in mTSB/10	805:833	Polysaccharides were produced to the highest extent in mTSB/10, and the biofilms obtained were the densest in this medium.
31177828	1	17	theme	10-fold	338:344	arg1	media					331:335	three nutritive media	315:335	three nutritive media (10-fold diluted MRS, AOAC, and mTSB)	315:373	In this work, infrared spectroscopy was used to monitor the changes in the biochemical composition of biofilms of the probiotic bacterium Lactobacillus rhamnosus GG (LGG) in three nutritive media (10-fold diluted MRS, AOAC, and mTSB), in situ and under flow conditions.
31177828	1	17	theme	10-fold	338:344	arg1	MRS					354:356	10-fold diluted MRS	338:356	10-fold diluted MRS	338:356	In this work, infrared spectroscopy was used to monitor the changes in the biochemical composition of biofilms of the probiotic bacterium Lactobacillus rhamnosus GG (LGG) in three nutritive media (10-fold diluted MRS, AOAC, and mTSB), in situ and under flow conditions.
31177828	1	18	theme	flow	394:397	arg1	conditions					399:408	flow conditions	394:408	flow conditions	394:408	In this work, infrared spectroscopy was used to monitor the changes in the biochemical composition of biofilms of the probiotic bacterium Lactobacillus rhamnosus GG (LGG) in three nutritive media (10-fold diluted MRS, AOAC, and mTSB), in situ and under flow conditions.
31177828	1	19	theme	infrared	155:162	arg1	spectroscopy					164:175	infrared spectroscopy	155:175	infrared spectroscopy	155:175	In this work, infrared spectroscopy was used to monitor the changes in the biochemical composition of biofilms of the probiotic bacterium Lactobacillus rhamnosus GG (LGG) in three nutritive media (10-fold diluted MRS, AOAC, and mTSB), in situ and under flow conditions.
31177828	0	20	theme	In	0:1	arg1	analysis					22:29	In situ spectroscopic analysis	0:29	In situ spectroscopic analysis of Lactobacillus rhamnosus GG flow on an abiotic surface	0:86	In situ spectroscopic analysis of Lactobacillus rhamnosus GG flow on an abiotic surface reveals a role for nutrients in biofilm development.
31177828	2	21	theme	LGG	471:473	arg1	cells					475:479	LGG cells	471:479	LGG cells	471:479	Epifluorescence microscopy was used to observe the shape of LGG cells and their distribution on the surface.
31177828	6	22	theme	rod	899:901	arg1	shape					903:907	The rod shape	895:907	The rod shape of the cells	895:920	The rod shape of the cells was preserved in MRS/10, whereas acidic stress induced in AOAC/10 and the nutritional quality of mTSB/10 led to strong morphological changes.
31177828	0	23	theme	spectroscopic	8:20	arg1	analysis					22:29	In situ spectroscopic analysis	0:29	In situ spectroscopic analysis of Lactobacillus rhamnosus GG flow on an abiotic surface	0:86	In situ spectroscopic analysis of Lactobacillus rhamnosus GG flow on an abiotic surface reveals a role for nutrients in biofilm development.
31177828	1	24	theme	probiotic	259:267	arg1	LGG					307:309	LGG	307:309	LGG	307:309	In this work, infrared spectroscopy was used to monitor the changes in the biochemical composition of biofilms of the probiotic bacterium Lactobacillus rhamnosus GG (LGG) in three nutritive media (10-fold diluted MRS, AOAC, and mTSB), in situ and under flow conditions.
31177828	1	24	theme	probiotic	259:267	arg1	bacterium					269:277	the probiotic bacterium	255:277	the probiotic bacterium Lactobacillus rhamnosus GG (LGG)	255:310	In this work, infrared spectroscopy was used to monitor the changes in the biochemical composition of biofilms of the probiotic bacterium Lactobacillus rhamnosus GG (LGG) in three nutritive media (10-fold diluted MRS, AOAC, and mTSB), in situ and under flow conditions.
31177828	2	25	theme	cells	475:479	arg1	distribution					491:502	their distribution	485:502	their distribution on the surface	485:517	Epifluorescence microscopy was used to observe the shape of LGG cells and their distribution on the surface.
31177828	2	25	theme	cells	475:479	arg1	shape					462:466	the shape	458:466	the shape of LGG cells	458:479	Epifluorescence microscopy was used to observe the shape of LGG cells and their distribution on the surface.
31177828	1	26	theme	diluted	346:352	arg1	media					331:335	three nutritive media	315:335	three nutritive media (10-fold diluted MRS, AOAC, and mTSB)	315:373	In this work, infrared spectroscopy was used to monitor the changes in the biochemical composition of biofilms of the probiotic bacterium Lactobacillus rhamnosus GG (LGG) in three nutritive media (10-fold diluted MRS, AOAC, and mTSB), in situ and under flow conditions.
31177828	1	26	theme	diluted	346:352	arg1	MRS					354:356	10-fold diluted MRS	338:356	10-fold diluted MRS	338:356	In this work, infrared spectroscopy was used to monitor the changes in the biochemical composition of biofilms of the probiotic bacterium Lactobacillus rhamnosus GG (LGG) in three nutritive media (10-fold diluted MRS, AOAC, and mTSB), in situ and under flow conditions.
31177828	7	27	theme	LGG	1164:1166	arg1	biofilms					1168:1175	LGG biofilms	1164:1175	LGG biofilms	1164:1175	These alterations due to the nutritive environment are important to consider in research and use of LGG biofilms.
31177828	0	28	theme	Lactobacillus	34:46	arg1	flow					61:64	Lactobacillus rhamnosus GG flow	34:64	Lactobacillus rhamnosus GG flow	34:64	In situ spectroscopic analysis of Lactobacillus rhamnosus GG flow on an abiotic surface reveals a role for nutrients in biofilm development.
31177828	1	29	theme	bacterium	269:277	arg1	biofilms					243:250	biofilms	243:250	biofilms of the probiotic bacterium Lactobacillus rhamnosus GG (LGG)	243:310	In this work, infrared spectroscopy was used to monitor the changes in the biochemical composition of biofilms of the probiotic bacterium Lactobacillus rhamnosus GG (LGG) in three nutritive media (10-fold diluted MRS, AOAC, and mTSB), in situ and under flow conditions.
31177828	0	30	theme	biofilm	120:126	arg1	development					128:138	biofilm development	120:138	biofilm development	120:138	In situ spectroscopic analysis of Lactobacillus rhamnosus GG flow on an abiotic surface reveals a role for nutrients in biofilm development.
31177828	3	31	theme	Spectroscopic	520:532	arg1	fingerprints					534:545	Spectroscopic fingerprints	520:545	Spectroscopic fingerprints recorded as a function of time	520:576	Spectroscopic fingerprints recorded as a function of time revealed a medium-dependent content of nucleic acids, phospholipids and polysaccharides in the biofilms.
31177828	6	32	theme	acidic	955:960	arg1	stress					962:967	acidic stress	955:967	acidic stress induced in AOAC/10	955:986	The rod shape of the cells was preserved in MRS/10, whereas acidic stress induced in AOAC/10 and the nutritional quality of mTSB/10 led to strong morphological changes.
31177828	0	33	theme	GG	58:59	arg1	flow					61:64	Lactobacillus rhamnosus GG flow	34:64	Lactobacillus rhamnosus GG flow	34:64	In situ spectroscopic analysis of Lactobacillus rhamnosus GG flow on an abiotic surface reveals a role for nutrients in biofilm development.
31177828	3	34	theme	time	573:576	arg1	function					561:568	a function	559:568	a function of time	559:576	Spectroscopic fingerprints recorded as a function of time revealed a medium-dependent content of nucleic acids, phospholipids and polysaccharides in the biofilms.
31177828	1	35	used	used	181:184	arg2	spectroscopy					164:175	infrared spectroscopy	155:175	infrared spectroscopy	155:175	In this work, infrared spectroscopy was used to monitor the changes in the biochemical composition of biofilms of the probiotic bacterium Lactobacillus rhamnosus GG (LGG) in three nutritive media (10-fold diluted MRS, AOAC, and mTSB), in situ and under flow conditions.
31177828	6	36	theme	morphological	1041:1053	arg1	changes					1055:1061	strong morphological changes	1034:1061	strong morphological changes	1034:1061	The rod shape of the cells was preserved in MRS/10, whereas acidic stress induced in AOAC/10 and the nutritional quality of mTSB/10 led to strong morphological changes.
31177828	0	37	theme	rhamnosus	48:56	arg1	flow					61:64	Lactobacillus rhamnosus GG flow	34:64	Lactobacillus rhamnosus GG flow	34:64	In situ spectroscopic analysis of Lactobacillus rhamnosus GG flow on an abiotic surface reveals a role for nutrients in biofilm development.
31177828	3	38	theme	phospholipids	632:644	arg1	content					606:612	a medium-dependent content	587:612	a medium-dependent content of nucleic acids, phospholipids and polysaccharides in the biofilms	587:680	Spectroscopic fingerprints recorded as a function of time revealed a medium-dependent content of nucleic acids, phospholipids and polysaccharides in the biofilms.
31177828	0	39	from	role	98:101	arg1	development					128:138	biofilm development	120:138	biofilm development	120:138	In situ spectroscopic analysis of Lactobacillus rhamnosus GG flow on an abiotic surface reveals a role for nutrients in biofilm development.
31177828	6	40	theme	strong	1034:1039	arg1	changes					1055:1061	strong morphological changes	1034:1061	strong morphological changes	1034:1061	The rod shape of the cells was preserved in MRS/10, whereas acidic stress induced in AOAC/10 and the nutritional quality of mTSB/10 led to strong morphological changes.
31177828	1	41	dep	media	331:335	arg1	media					331:335	three nutritive media	315:335	three nutritive media (10-fold diluted MRS, AOAC, and mTSB)	315:373	In this work, infrared spectroscopy was used to monitor the changes in the biochemical composition of biofilms of the probiotic bacterium Lactobacillus rhamnosus GG (LGG) in three nutritive media (10-fold diluted MRS, AOAC, and mTSB), in situ and under flow conditions.
31177828	1	41	dep	media	331:335	arg1	AOAC					359:362	AOAC	359:362	AOAC	359:362	In this work, infrared spectroscopy was used to monitor the changes in the biochemical composition of biofilms of the probiotic bacterium Lactobacillus rhamnosus GG (LGG) in three nutritive media (10-fold diluted MRS, AOAC, and mTSB), in situ and under flow conditions.
31177828	1	41	dep	media	331:335	arg1	mTSB					369:372	mTSB	369:372	mTSB	369:372	In this work, infrared spectroscopy was used to monitor the changes in the biochemical composition of biofilms of the probiotic bacterium Lactobacillus rhamnosus GG (LGG) in three nutritive media (10-fold diluted MRS, AOAC, and mTSB), in situ and under flow conditions.
31177828	1	41	dep	media	331:335	arg1	MRS					354:356	10-fold diluted MRS	338:356	10-fold diluted MRS	338:356	In this work, infrared spectroscopy was used to monitor the changes in the biochemical composition of biofilms of the probiotic bacterium Lactobacillus rhamnosus GG (LGG) in three nutritive media (10-fold diluted MRS, AOAC, and mTSB), in situ and under flow conditions.
31177828	3	42	theme	nucleic	617:623	arg1	acids					625:629	nucleic acids	617:629	nucleic acids	617:629	Spectroscopic fingerprints recorded as a function of time revealed a medium-dependent content of nucleic acids, phospholipids and polysaccharides in the biofilms.
31177828	5	43	from	medium	887:892	arg1	densest					871:877	densest	871:877	densest	871:877	Polysaccharides were produced to the highest extent in mTSB/10, and the biofilms obtained were the densest in this medium.
31177828	5	43	from	medium	887:892	arg1	biofilms					844:851	the biofilms	840:851	the biofilms obtained	840:860	Polysaccharides were produced to the highest extent in mTSB/10, and the biofilms obtained were the densest in this medium.
31177828	1	44	from	changes	201:207	arg1	composition					228:238	the biochemical composition	212:238	the biochemical composition of biofilms of the probiotic bacterium Lactobacillus rhamnosus GG (LGG)	212:310	In this work, infrared spectroscopy was used to monitor the changes in the biochemical composition of biofilms of the probiotic bacterium Lactobacillus rhamnosus GG (LGG) in three nutritive media (10-fold diluted MRS, AOAC, and mTSB), in situ and under flow conditions.
31177828	1	44	from	changes	201:207	arg1	AOAC					359:362	AOAC	359:362	AOAC	359:362	In this work, infrared spectroscopy was used to monitor the changes in the biochemical composition of biofilms of the probiotic bacterium Lactobacillus rhamnosus GG (LGG) in three nutritive media (10-fold diluted MRS, AOAC, and mTSB), in situ and under flow conditions.
31177828	1	44	from	changes	201:207	arg1	mTSB					369:372	mTSB	369:372	mTSB	369:372	In this work, infrared spectroscopy was used to monitor the changes in the biochemical composition of biofilms of the probiotic bacterium Lactobacillus rhamnosus GG (LGG) in three nutritive media (10-fold diluted MRS, AOAC, and mTSB), in situ and under flow conditions.
31177828	1	44	from	changes	201:207	arg1	MRS					354:356	10-fold diluted MRS	338:356	10-fold diluted MRS	338:356	In this work, infrared spectroscopy was used to monitor the changes in the biochemical composition of biofilms of the probiotic bacterium Lactobacillus rhamnosus GG (LGG) in three nutritive media (10-fold diluted MRS, AOAC, and mTSB), in situ and under flow conditions.
31177828	1	44	from	changes	201:207	arg1	media					331:335	three nutritive media	315:335	three nutritive media (10-fold diluted MRS, AOAC, and mTSB)	315:373	In this work, infrared spectroscopy was used to monitor the changes in the biochemical composition of biofilms of the probiotic bacterium Lactobacillus rhamnosus GG (LGG) in three nutritive media (10-fold diluted MRS, AOAC, and mTSB), in situ and under flow conditions.
31177828	2	45	from	shape	462:466	arg1	surface					511:517	the surface	507:517	the surface	507:517	Epifluorescence microscopy was used to observe the shape of LGG cells and their distribution on the surface.
31177828	4	46	theme	acid	731:734	arg1	synthesis					711:719	time-dependent synthesis	696:719	time-dependent synthesis of lactic acid	696:734	In addition, time-dependent synthesis of lactic acid was observed in MRS/10 and AOAC/10.
31177828	5	47	from	extent	817:822	arg1	mTSB/10					827:833	mTSB/10	827:833	mTSB/10	827:833	Polysaccharides were produced to the highest extent in mTSB/10, and the biofilms obtained were the densest in this medium.
31177828	5	48	from	densest	871:877	arg1	medium					887:892	this medium	882:892	this medium	882:892	Polysaccharides were produced to the highest extent in mTSB/10, and the biofilms obtained were the densest in this medium.
31177828	2	49	used	used	442:445	arg2	microscopy					427:436	Epifluorescence microscopy	411:436	Epifluorescence microscopy	411:436	Epifluorescence microscopy was used to observe the shape of LGG cells and their distribution on the surface.
31177828	6	50	theme	mTSB/10	1019:1025	arg1	quality					1008:1014	the nutritional quality	992:1014	the nutritional quality of mTSB/10	992:1025	The rod shape of the cells was preserved in MRS/10, whereas acidic stress induced in AOAC/10 and the nutritional quality of mTSB/10 led to strong morphological changes.
31177828	0	51	theme	flow	61:64	arg1	analysis					22:29	In situ spectroscopic analysis	0:29	In situ spectroscopic analysis of Lactobacillus rhamnosus GG flow on an abiotic surface	0:86	In situ spectroscopic analysis of Lactobacillus rhamnosus GG flow on an abiotic surface reveals a role for nutrients in biofilm development.
31177828	0	52	from	analysis	22:29	arg1	surface					80:86	an abiotic surface	69:86	an abiotic surface	69:86	In situ spectroscopic analysis of Lactobacillus rhamnosus GG flow on an abiotic surface reveals a role for nutrients in biofilm development.
31177828	0	53	dep	In	0:1	arg1	situ					3:6	situ	3:6	situ	3:6	In situ spectroscopic analysis of Lactobacillus rhamnosus GG flow on an abiotic surface reveals a role for nutrients in biofilm development.
31177828	7	54	theme	biofilms	1168:1175	arg1	use					1157:1159	use	1157:1159	use	1157:1159	These alterations due to the nutritive environment are important to consider in research and use of LGG biofilms.
31177828	7	54	theme	biofilms	1168:1175	arg1	research					1144:1151	research	1144:1151	research	1144:1151	These alterations due to the nutritive environment are important to consider in research and use of LGG biofilms.
30308517	4	0	from	effects	639:645	arg1	ARTI					650:653	ARTI	650:653	ARTI	650:653	In this study, we aimed to systematically explore its composition and the mechanism of its effects in ARTI.
30308517	12	1	located	observed	1772:1779	arg1	types					1815:1819	the untreated PAK-induced mice types	1784:1819	the untreated PAK-induced mice types	1784:1819	We found that the anti-inflammatory role of verbenalin was attenuated in GPR18 knockout mice compared with wild-type mice, although no statistically significant difference was observed in the untreated PAK-induced mice types.
30308517	12	1	located	observed	1772:1779	arg2	difference					1757:1766	no statistically significant difference	1728:1766	no statistically significant difference	1728:1766	We found that the anti-inflammatory role of verbenalin was attenuated in GPR18 knockout mice compared with wild-type mice, although no statistically significant difference was observed in the untreated PAK-induced mice types.
30308517	9	2	theme	inflammatory	1225:1236	arg1	factors					1238:1244	inflammatory factors	1225:1244	inflammatory factors	1225:1244	RESULTS SJC significantly inhibited the expression and secretion of inflammatory factors from PAK-induced mouse lung tissues or lipopolysaccharide-induced peritoneal macrophages.
30308517	2	3	theme	important	374:382	arg1	antibiotics					319:329	antibiotics	319:329	antibiotics used for ARTI	319:343	Although powerful and significant in the treatment of infections, antibiotics used for ARTI inappropriately have been an important contributor to antibiotic resistance.
30308517	2	3	theme	important	374:382	arg1	contributor					384:394	an important contributor	371:394	an important contributor to antibiotic resistance	371:419	Although powerful and significant in the treatment of infections, antibiotics used for ARTI inappropriately have been an important contributor to antibiotic resistance.
30308517	12	4	theme	untreated	1788:1796	arg1	types					1815:1819	the untreated PAK-induced mice types	1784:1819	the untreated PAK-induced mice types	1784:1819	We found that the anti-inflammatory role of verbenalin was attenuated in GPR18 knockout mice compared with wild-type mice, although no statistically significant difference was observed in the untreated PAK-induced mice types.
30308517	5	5	theme	different	773:781	arg1	drugs					783:787	different drugs	773:787	different drugs	773:787	METHODS Pseudomonas aeruginosa (PAK) strain was used to generate a mouse model of ARTI, which were then treated with different drugs or compounds to determine the corresponding anti-inflammatory roles.
30308517	9	6	theme	factors	1238:1244	arg1	secretion					1212:1220	secretion	1212:1220	secretion	1212:1220	RESULTS SJC significantly inhibited the expression and secretion of inflammatory factors from PAK-induced mouse lung tissues or lipopolysaccharide-induced peritoneal macrophages.
30308517	9	6	theme	factors	1238:1244	arg1	expression					1197:1206	expression	1197:1206	expression	1197:1206	RESULTS SJC significantly inhibited the expression and secretion of inflammatory factors from PAK-induced mouse lung tissues or lipopolysaccharide-induced peritoneal macrophages.
30308517	9	7	theme	peritoneal	1312:1321	arg1	macrophages					1323:1333	lipopolysaccharide-induced peritoneal macrophages	1285:1333	lipopolysaccharide-induced peritoneal macrophages	1285:1333	RESULTS SJC significantly inhibited the expression and secretion of inflammatory factors from PAK-induced mouse lung tissues or lipopolysaccharide-induced peritoneal macrophages.
30308517	5	8	theme	ARTI	738:741	arg1	model					729:733	a mouse model	721:733	a mouse model	721:733	METHODS Pseudomonas aeruginosa (PAK) strain was used to generate a mouse model of ARTI, which were then treated with different drugs or compounds to determine the corresponding anti-inflammatory roles.
30308517	8	9	theme	lung	1018:1021	arg1	tissues					1023:1029	the lung tissues	1014:1029	the lung tissues of mice	1014:1037	RNAs from the lung tissues of mice were prepared for microarray analysis to reveal globally altered genes and the pathways involved after SJC treatment.
30308517	2	10	theme	infections	307:316	arg1	treatment					294:302	the treatment	290:302	the treatment of infections	290:316	Although powerful and significant in the treatment of infections, antibiotics used for ARTI inappropriately have been an important contributor to antibiotic resistance.
30308517	11	11	theme	different	1520:1528	arg1	groups					1540:1545	the different treatment groups	1516:1545	the different treatment groups	1516:1545	Microarray data revealed numerous differentially expressed genes among the different treatment groups; here, we focused on studying the role of GPR18.
30308517	12	12	theme	verbenalin	1640:1649	arg1	role					1632:1635	the anti-inflammatory role	1610:1635	the anti-inflammatory role of verbenalin	1610:1649	We found that the anti-inflammatory role of verbenalin was attenuated in GPR18 knockout mice compared with wild-type mice, although no statistically significant difference was observed in the untreated PAK-induced mice types.
30308517	0	13	theme	GPR18	109:113	arg1	Activation					95:104	Activation	95:104	Activation of GPR18 by Verbenalin	95:127	Shufeng Jiedu Capsules Alleviate Lipopolysaccharide-Induced Acute Lung Inflammatory Injury via Activation of GPR18 by Verbenalin.
30308517	8	14	theme	mice	1034:1037	arg1	tissues					1023:1029	the lung tissues	1014:1029	the lung tissues of mice	1014:1037	RNAs from the lung tissues of mice were prepared for microarray analysis to reveal globally altered genes and the pathways involved after SJC treatment.
30308517	9	15	from	macrophages	1323:1333	arg1	secretion					1212:1220	secretion	1212:1220	secretion	1212:1220	RESULTS SJC significantly inhibited the expression and secretion of inflammatory factors from PAK-induced mouse lung tissues or lipopolysaccharide-induced peritoneal macrophages.
30308517	9	15	from	macrophages	1323:1333	arg1	expression					1197:1206	expression	1197:1206	expression	1197:1206	RESULTS SJC significantly inhibited the expression and secretion of inflammatory factors from PAK-induced mouse lung tissues or lipopolysaccharide-induced peritoneal macrophages.
30308517	9	16	from	tissues	1274:1280	arg1	secretion					1212:1220	secretion	1212:1220	secretion	1212:1220	RESULTS SJC significantly inhibited the expression and secretion of inflammatory factors from PAK-induced mouse lung tissues or lipopolysaccharide-induced peritoneal macrophages.
30308517	9	16	from	tissues	1274:1280	arg1	expression					1197:1206	expression	1197:1206	expression	1197:1206	RESULTS SJC significantly inhibited the expression and secretion of inflammatory factors from PAK-induced mouse lung tissues or lipopolysaccharide-induced peritoneal macrophages.
30308517	4	17	from	composition	602:612	arg1	ARTI					650:653	ARTI	650:653	ARTI	650:653	In this study, we aimed to systematically explore its composition and the mechanism of its effects in ARTI.
30308517	9	18	dep	expression	1197:1206	arg1	the					1193:1195	the	1193:1195	the	1193:1195	RESULTS SJC significantly inhibited the expression and secretion of inflammatory factors from PAK-induced mouse lung tissues or lipopolysaccharide-induced peritoneal macrophages.
30308517	5	19	dep	METHODS	656:662	arg1	used					704:707	used	704:707	was used to generate a mouse model of ARTI, which were then treated with different drugs or compounds to determine the corresponding anti-inflammatory roles	700:855	METHODS Pseudomonas aeruginosa (PAK) strain was used to generate a mouse model of ARTI, which were then treated with different drugs or compounds to determine the corresponding anti-inflammatory roles.
30308517	6	20	theme	spectrometry	935:946	arg1	time					908:911	High-performance liquid chromatography-quadrupole time	858:911	High-performance liquid chromatography-quadrupole time of flight-tandem mass spectrometry	858:946	High-performance liquid chromatography-quadrupole time of flight-tandem mass spectrometry.
30308517	8	21	theme	altered	1096:1102	arg1	genes					1104:1108	globally altered genes	1087:1108	globally altered genes	1087:1108	RNAs from the lung tissues of mice were prepared for microarray analysis to reveal globally altered genes and the pathways involved after SJC treatment.
30308517	9	22	theme	lung	1269:1272	arg1	tissues					1274:1280	PAK-induced mouse lung tissues	1251:1280	PAK-induced mouse lung tissues	1251:1280	RESULTS SJC significantly inhibited the expression and secretion of inflammatory factors from PAK-induced mouse lung tissues or lipopolysaccharide-induced peritoneal macrophages.
30308517	10	23	theme	compounds	1369:1377	arg1	one					1348:1350	one	1348:1350	one	1348:1350	Verbenalin, one of the bioactive compounds identified in SJC, also showed notable anti-inflammatory effects.
30308517	10	23	theme	compounds	1369:1377	arg1	Verbenalin					1336:1345	Verbenalin	1336:1345	Verbenalin	1336:1345	Verbenalin, one of the bioactive compounds identified in SJC, also showed notable anti-inflammatory effects.
30308517	10	23	theme	compounds	1369:1377	arg1	compounds					1369:1377	the bioactive compounds	1355:1377	the bioactive compounds identified in SJC	1355:1395	Verbenalin, one of the bioactive compounds identified in SJC, also showed notable anti-inflammatory effects.
30308517	0	24	theme	Jiedu	8:12	arg1	Capsules					14:21	Shufeng Jiedu Capsules	0:21	Shufeng Jiedu Capsules	0:21	Shufeng Jiedu Capsules Alleviate Lipopolysaccharide-Induced Acute Lung Inflammatory Injury via Activation of GPR18 by Verbenalin.
30308517	6	25	theme	mass	930:933	arg1	spectrometry					935:946	flight-tandem mass spectrometry	916:946	flight-tandem mass spectrometry	916:946	High-performance liquid chromatography-quadrupole time of flight-tandem mass spectrometry.
30308517	13	26	theme	anti-inflammatory	1947:1963	arg1	compound					1965:1972	a significant anti-inflammatory compound	1933:1972	a significant anti-inflammatory compound	1933:1972	CONCLUSION Our data not only showed the chemical composition of SJC, but also demonstrated that verbenalin was a significant anti-inflammatory compound, which may function through GPR18.
30308517	13	26	theme	anti-inflammatory	1947:1963	arg1	verbenalin					1918:1927	verbenalin	1918:1927	verbenalin	1918:1927	CONCLUSION Our data not only showed the chemical composition of SJC, but also demonstrated that verbenalin was a significant anti-inflammatory compound, which may function through GPR18.
30308517	13	27	dep	CONCLUSION	1822:1831	arg1	showed					1851:1856	showed	1851:1856	showed the chemical composition of SJC	1851:1888	CONCLUSION Our data not only showed the chemical composition of SJC, but also demonstrated that verbenalin was a significant anti-inflammatory compound, which may function through GPR18.
30308517	13	27	dep	CONCLUSION	1822:1831	arg1	demonstrated					1900:1911	demonstrated	1900:1911	demonstrated that verbenalin was a significant anti-inflammatory compound, which may function through GPR18	1900:2006	CONCLUSION Our data not only showed the chemical composition of SJC, but also demonstrated that verbenalin was a significant anti-inflammatory compound, which may function through GPR18.
30308517	10	28	theme	bioactive	1359:1367	arg1	compounds					1369:1377	the bioactive compounds	1355:1377	the bioactive compounds identified in SJC	1355:1395	Verbenalin, one of the bioactive compounds identified in SJC, also showed notable anti-inflammatory effects.
30308517	3	29	theme	Jiedu	458:462	arg1	SJC					473:475	SJC	473:475	SJC	473:475	We previously reported that Shufeng Jiedu Capsule (SJC) can effectively amplify anti-inflammatory signaling during infection.
30308517	3	29	theme	Jiedu	458:462	arg1	Capsule					464:470	Shufeng Jiedu Capsule	450:470	Shufeng Jiedu Capsule (SJC)	450:476	We previously reported that Shufeng Jiedu Capsule (SJC) can effectively amplify anti-inflammatory signaling during infection.
30308517	0	30	theme	Shufeng	0:6	arg1	Capsules					14:21	Shufeng Jiedu Capsules	0:21	Shufeng Jiedu Capsules	0:21	Shufeng Jiedu Capsules Alleviate Lipopolysaccharide-Induced Acute Lung Inflammatory Injury via Activation of GPR18 by Verbenalin.
30308517	12	31	theme	wild-type	1703:1711	arg1	mice					1713:1716	wild-type mice	1703:1716	wild-type mice	1703:1716	We found that the anti-inflammatory role of verbenalin was attenuated in GPR18 knockout mice compared with wild-type mice, although no statistically significant difference was observed in the untreated PAK-induced mice types.
30308517	5	32	theme	aeruginosa	676:685	arg1	strain					693:698	Pseudomonas aeruginosa (PAK) strain	664:698	Pseudomonas aeruginosa (PAK) strain	664:698	METHODS Pseudomonas aeruginosa (PAK) strain was used to generate a mouse model of ARTI, which were then treated with different drugs or compounds to determine the corresponding anti-inflammatory roles.
30308517	7	33	theme	chemical	977:984	arg1	compounds					986:994	the chemical compounds	973:994	the chemical compounds in SJC	973:1001	was conducted to detect the chemical compounds in SJC.
30308517	13	34	theme	significant	1935:1945	arg1	compound					1965:1972	a significant anti-inflammatory compound	1933:1972	a significant anti-inflammatory compound	1933:1972	CONCLUSION Our data not only showed the chemical composition of SJC, but also demonstrated that verbenalin was a significant anti-inflammatory compound, which may function through GPR18.
30308517	13	34	theme	significant	1935:1945	arg1	verbenalin					1918:1927	verbenalin	1918:1927	verbenalin	1918:1927	CONCLUSION Our data not only showed the chemical composition of SJC, but also demonstrated that verbenalin was a significant anti-inflammatory compound, which may function through GPR18.
30308517	2	35	theme	antibiotic	399:408	arg1	resistance					410:419	antibiotic resistance	399:419	antibiotic resistance	399:419	Although powerful and significant in the treatment of infections, antibiotics used for ARTI inappropriately have been an important contributor to antibiotic resistance.
30308517	5	36	used	used	704:707	arg2	strain					693:698	Pseudomonas aeruginosa (PAK) strain	664:698	Pseudomonas aeruginosa (PAK) strain	664:698	METHODS Pseudomonas aeruginosa (PAK) strain was used to generate a mouse model of ARTI, which were then treated with different drugs or compounds to determine the corresponding anti-inflammatory roles.
30308517	8	37	theme	microarray	1057:1066	arg1	analysis					1068:1075	microarray analysis	1057:1075	microarray analysis to reveal globally altered genes and the pathways involved after SJC treatment	1057:1154	RNAs from the lung tissues of mice were prepared for microarray analysis to reveal globally altered genes and the pathways involved after SJC treatment.
30308517	9	38	theme	mouse	1263:1267	arg1	tissues					1274:1280	PAK-induced mouse lung tissues	1251:1280	PAK-induced mouse lung tissues	1251:1280	RESULTS SJC significantly inhibited the expression and secretion of inflammatory factors from PAK-induced mouse lung tissues or lipopolysaccharide-induced peritoneal macrophages.
30308517	2	39	from	powerful	262:269	arg1	treatment					294:302	the treatment	290:302	the treatment of infections	290:316	Although powerful and significant in the treatment of infections, antibiotics used for ARTI inappropriately have been an important contributor to antibiotic resistance.
30308517	13	40	theme	chemical	1862:1869	arg1	composition					1871:1881	the chemical composition	1858:1881	the chemical composition of SJC	1858:1888	CONCLUSION Our data not only showed the chemical composition of SJC, but also demonstrated that verbenalin was a significant anti-inflammatory compound, which may function through GPR18.
30308517	5	41	theme	anti-inflammatory	833:849	arg1	roles					851:855	the corresponding anti-inflammatory roles	815:855	the corresponding anti-inflammatory roles	815:855	METHODS Pseudomonas aeruginosa (PAK) strain was used to generate a mouse model of ARTI, which were then treated with different drugs or compounds to determine the corresponding anti-inflammatory roles.
30308517	12	42	theme	anti-inflammatory	1614:1630	arg1	role					1632:1635	the anti-inflammatory role	1610:1635	the anti-inflammatory role of verbenalin	1610:1649	We found that the anti-inflammatory role of verbenalin was attenuated in GPR18 knockout mice compared with wild-type mice, although no statistically significant difference was observed in the untreated PAK-induced mice types.
30308517	5	43	theme	mouse	723:727	arg1	model					729:733	a mouse model	721:733	a mouse model	721:733	METHODS Pseudomonas aeruginosa (PAK) strain was used to generate a mouse model of ARTI, which were then treated with different drugs or compounds to determine the corresponding anti-inflammatory roles.
30308517	6	44	theme	flight-tandem	916:928	arg1	spectrometry					935:946	flight-tandem mass spectrometry	916:946	flight-tandem mass spectrometry	916:946	High-performance liquid chromatography-quadrupole time of flight-tandem mass spectrometry.
30308517	12	45	theme	significant	1745:1755	arg1	difference					1757:1766	no statistically significant difference	1728:1766	no statistically significant difference	1728:1766	We found that the anti-inflammatory role of verbenalin was attenuated in GPR18 knockout mice compared with wild-type mice, although no statistically significant difference was observed in the untreated PAK-induced mice types.
30308517	9	46	theme	RESULTS	1157:1163	arg1	SJC					1165:1167	RESULTS SJC	1157:1167	RESULTS SJC	1157:1167	RESULTS SJC significantly inhibited the expression and secretion of inflammatory factors from PAK-induced mouse lung tissues or lipopolysaccharide-induced peritoneal macrophages.
30308517	0	47	theme	Acute	60:64	arg1	Injury					84:89	Lipopolysaccharide-Induced Acute Lung Inflammatory Injury	33:89	Lipopolysaccharide-Induced Acute Lung Inflammatory Injury	33:89	Shufeng Jiedu Capsules Alleviate Lipopolysaccharide-Induced Acute Lung Inflammatory Injury via Activation of GPR18 by Verbenalin.
30308517	7	48	from	compounds	986:994	arg1	SJC					999:1001	SJC	999:1001	SJC	999:1001	was conducted to detect the chemical compounds in SJC.
30308517	12	49	theme	knockout	1675:1682	arg1	mice					1684:1687	GPR18 knockout mice	1669:1687	GPR18 knockout mice	1669:1687	We found that the anti-inflammatory role of verbenalin was attenuated in GPR18 knockout mice compared with wild-type mice, although no statistically significant difference was observed in the untreated PAK-induced mice types.
30308517	1	50	theme	common	199:204	arg1	infection					170:178	BACKGROUND/AIMS Acute respiratory tract infection	130:178	BACKGROUND/AIMS Acute respiratory tract infection (ARTI)	130:185	BACKGROUND/AIMS Acute respiratory tract infection (ARTI) is the most common reason for outpatient physician office visits.
30308517	1	50	theme	common	199:204	arg1	reason					206:211	the most common reason	190:211	the most common reason for outpatient physician office visits	190:250	BACKGROUND/AIMS Acute respiratory tract infection (ARTI) is the most common reason for outpatient physician office visits.
30308517	1	51	theme	outpatient	217:226	arg1	visits					245:250	outpatient physician office visits	217:250	outpatient physician office visits	217:250	BACKGROUND/AIMS Acute respiratory tract infection (ARTI) is the most common reason for outpatient physician office visits.
30308517	0	52	theme	Lipopolysaccharide-Induced	33:58	arg1	Injury					84:89	Lipopolysaccharide-Induced Acute Lung Inflammatory Injury	33:89	Lipopolysaccharide-Induced Acute Lung Inflammatory Injury	33:89	Shufeng Jiedu Capsules Alleviate Lipopolysaccharide-Induced Acute Lung Inflammatory Injury via Activation of GPR18 by Verbenalin.
30308517	3	53	theme	Shufeng	450:456	arg1	SJC					473:475	SJC	473:475	SJC	473:475	We previously reported that Shufeng Jiedu Capsule (SJC) can effectively amplify anti-inflammatory signaling during infection.
30308517	3	53	theme	Shufeng	450:456	arg1	Capsule					464:470	Shufeng Jiedu Capsule	450:470	Shufeng Jiedu Capsule (SJC)	450:476	We previously reported that Shufeng Jiedu Capsule (SJC) can effectively amplify anti-inflammatory signaling during infection.
30308517	5	54	theme	corresponding	819:831	arg1	roles					851:855	the corresponding anti-inflammatory roles	815:855	the corresponding anti-inflammatory roles	815:855	METHODS Pseudomonas aeruginosa (PAK) strain was used to generate a mouse model of ARTI, which were then treated with different drugs or compounds to determine the corresponding anti-inflammatory roles.
30308517	1	55	theme	physician	228:236	arg1	visits					245:250	outpatient physician office visits	217:250	outpatient physician office visits	217:250	BACKGROUND/AIMS Acute respiratory tract infection (ARTI) is the most common reason for outpatient physician office visits.
30308517	0	56	theme	Inflammatory	71:82	arg1	Injury					84:89	Lipopolysaccharide-Induced Acute Lung Inflammatory Injury	33:89	Lipopolysaccharide-Induced Acute Lung Inflammatory Injury	33:89	Shufeng Jiedu Capsules Alleviate Lipopolysaccharide-Induced Acute Lung Inflammatory Injury via Activation of GPR18 by Verbenalin.
30308517	11	57	theme	treatment	1530:1538	arg1	groups					1540:1545	the different treatment groups	1516:1545	the different treatment groups	1516:1545	Microarray data revealed numerous differentially expressed genes among the different treatment groups; here, we focused on studying the role of GPR18.
30308517	9	58	theme	PAK-induced	1251:1261	arg1	tissues					1274:1280	PAK-induced mouse lung tissues	1251:1280	PAK-induced mouse lung tissues	1251:1280	RESULTS SJC significantly inhibited the expression and secretion of inflammatory factors from PAK-induced mouse lung tissues or lipopolysaccharide-induced peritoneal macrophages.
30308517	6	59	theme	chromatography-quadrupole	882:906	arg1	time					908:911	High-performance liquid chromatography-quadrupole time	858:911	High-performance liquid chromatography-quadrupole time of flight-tandem mass spectrometry	858:946	High-performance liquid chromatography-quadrupole time of flight-tandem mass spectrometry.
30308517	1	60	theme	BACKGROUND/AIMS	130:144	arg1	ARTI					181:184	ARTI	181:184	ARTI	181:184	BACKGROUND/AIMS Acute respiratory tract infection (ARTI) is the most common reason for outpatient physician office visits.
30308517	1	60	theme	BACKGROUND/AIMS	130:144	arg1	reason					206:211	the most common reason	190:211	the most common reason for outpatient physician office visits	190:250	BACKGROUND/AIMS Acute respiratory tract infection (ARTI) is the most common reason for outpatient physician office visits.
30308517	1	60	theme	BACKGROUND/AIMS	130:144	arg1	infection					170:178	BACKGROUND/AIMS Acute respiratory tract infection	130:178	BACKGROUND/AIMS Acute respiratory tract infection (ARTI)	130:185	BACKGROUND/AIMS Acute respiratory tract infection (ARTI) is the most common reason for outpatient physician office visits.
30308517	0	61	theme	Lung	66:69	arg1	Injury					84:89	Lipopolysaccharide-Induced Acute Lung Inflammatory Injury	33:89	Lipopolysaccharide-Induced Acute Lung Inflammatory Injury	33:89	Shufeng Jiedu Capsules Alleviate Lipopolysaccharide-Induced Acute Lung Inflammatory Injury via Activation of GPR18 by Verbenalin.
30308517	8	62	from	tissues	1023:1029	arg1	RNAs					1004:1007	RNAs	1004:1007	RNAs from the lung tissues of mice	1004:1037	RNAs from the lung tissues of mice were prepared for microarray analysis to reveal globally altered genes and the pathways involved after SJC treatment.
30308517	6	63	theme	liquid	875:880	arg1	time					908:911	High-performance liquid chromatography-quadrupole time	858:911	High-performance liquid chromatography-quadrupole time of flight-tandem mass spectrometry	858:946	High-performance liquid chromatography-quadrupole time of flight-tandem mass spectrometry.
30308517	10	64	theme	anti-inflammatory	1418:1434	arg1	effects					1436:1442	notable anti-inflammatory effects	1410:1442	notable anti-inflammatory effects	1410:1442	Verbenalin, one of the bioactive compounds identified in SJC, also showed notable anti-inflammatory effects.
30308517	1	65	theme	Acute	146:150	arg1	ARTI					181:184	ARTI	181:184	ARTI	181:184	BACKGROUND/AIMS Acute respiratory tract infection (ARTI) is the most common reason for outpatient physician office visits.
30308517	1	65	theme	Acute	146:150	arg1	reason					206:211	the most common reason	190:211	the most common reason for outpatient physician office visits	190:250	BACKGROUND/AIMS Acute respiratory tract infection (ARTI) is the most common reason for outpatient physician office visits.
30308517	1	65	theme	Acute	146:150	arg1	infection					170:178	BACKGROUND/AIMS Acute respiratory tract infection	130:178	BACKGROUND/AIMS Acute respiratory tract infection (ARTI)	130:185	BACKGROUND/AIMS Acute respiratory tract infection (ARTI) is the most common reason for outpatient physician office visits.
30308517	2	66	from	treatment	294:302	arg1	powerful					262:269	powerful	262:269	powerful	262:269	Although powerful and significant in the treatment of infections, antibiotics used for ARTI inappropriately have been an important contributor to antibiotic resistance.
30308517	9	67	theme	lipopolysaccharide-induced	1285:1310	arg1	macrophages					1323:1333	lipopolysaccharide-induced peritoneal macrophages	1285:1333	lipopolysaccharide-induced peritoneal macrophages	1285:1333	RESULTS SJC significantly inhibited the expression and secretion of inflammatory factors from PAK-induced mouse lung tissues or lipopolysaccharide-induced peritoneal macrophages.
30308517	11	68	theme	expressed	1494:1502	arg1	genes					1504:1508	numerous differentially expressed genes	1470:1508	numerous differentially expressed genes	1470:1508	Microarray data revealed numerous differentially expressed genes among the different treatment groups; here, we focused on studying the role of GPR18.
30308517	6	69	theme	High-performance	858:873	arg1	time					908:911	High-performance liquid chromatography-quadrupole time	858:911	High-performance liquid chromatography-quadrupole time of flight-tandem mass spectrometry	858:946	High-performance liquid chromatography-quadrupole time of flight-tandem mass spectrometry.
30308517	10	70	theme	notable	1410:1416	arg1	effects					1436:1442	notable anti-inflammatory effects	1410:1442	notable anti-inflammatory effects	1410:1442	Verbenalin, one of the bioactive compounds identified in SJC, also showed notable anti-inflammatory effects.
30308517	1	71	theme	respiratory	152:162	arg1	ARTI					181:184	ARTI	181:184	ARTI	181:184	BACKGROUND/AIMS Acute respiratory tract infection (ARTI) is the most common reason for outpatient physician office visits.
30308517	1	71	theme	respiratory	152:162	arg1	reason					206:211	the most common reason	190:211	the most common reason for outpatient physician office visits	190:250	BACKGROUND/AIMS Acute respiratory tract infection (ARTI) is the most common reason for outpatient physician office visits.
30308517	1	71	theme	respiratory	152:162	arg1	infection					170:178	BACKGROUND/AIMS Acute respiratory tract infection	130:178	BACKGROUND/AIMS Acute respiratory tract infection (ARTI)	130:185	BACKGROUND/AIMS Acute respiratory tract infection (ARTI) is the most common reason for outpatient physician office visits.
30308517	1	72	theme	office	238:243	arg1	visits					245:250	outpatient physician office visits	217:250	outpatient physician office visits	217:250	BACKGROUND/AIMS Acute respiratory tract infection (ARTI) is the most common reason for outpatient physician office visits.
30308517	11	73	theme	numerous	1470:1477	arg1	genes					1504:1508	numerous differentially expressed genes	1470:1508	numerous differentially expressed genes	1470:1508	Microarray data revealed numerous differentially expressed genes among the different treatment groups; here, we focused on studying the role of GPR18.
30308517	1	74	theme	tract	164:168	arg1	ARTI					181:184	ARTI	181:184	ARTI	181:184	BACKGROUND/AIMS Acute respiratory tract infection (ARTI) is the most common reason for outpatient physician office visits.
30308517	1	74	theme	tract	164:168	arg1	reason					206:211	the most common reason	190:211	the most common reason for outpatient physician office visits	190:250	BACKGROUND/AIMS Acute respiratory tract infection (ARTI) is the most common reason for outpatient physician office visits.
30308517	1	74	theme	tract	164:168	arg1	infection					170:178	BACKGROUND/AIMS Acute respiratory tract infection	130:178	BACKGROUND/AIMS Acute respiratory tract infection (ARTI)	130:185	BACKGROUND/AIMS Acute respiratory tract infection (ARTI) is the most common reason for outpatient physician office visits.
30308517	3	75	theme	anti-inflammatory	502:518	arg1	signaling					520:528	anti-inflammatory signaling	502:528	anti-inflammatory signaling	502:528	We previously reported that Shufeng Jiedu Capsule (SJC) can effectively amplify anti-inflammatory signaling during infection.
30308517	4	76	from	ARTI	650:653	arg1	mechanism					622:630	the mechanism	618:630	the mechanism of its effects in ARTI	618:653	In this study, we aimed to systematically explore its composition and the mechanism of its effects in ARTI.
30308517	4	76	from	ARTI	650:653	arg1	composition					602:612	its composition	598:612	its composition	598:612	In this study, we aimed to systematically explore its composition and the mechanism of its effects in ARTI.
30308517	12	77	theme	mice	1810:1813	arg1	types					1815:1819	the untreated PAK-induced mice types	1784:1819	the untreated PAK-induced mice types	1784:1819	We found that the anti-inflammatory role of verbenalin was attenuated in GPR18 knockout mice compared with wild-type mice, although no statistically significant difference was observed in the untreated PAK-induced mice types.
30308517	12	78	theme	GPR18	1669:1673	arg1	mice					1684:1687	GPR18 knockout mice	1669:1687	GPR18 knockout mice	1669:1687	We found that the anti-inflammatory role of verbenalin was attenuated in GPR18 knockout mice compared with wild-type mice, although no statistically significant difference was observed in the untreated PAK-induced mice types.
30308517	11	79	theme	Microarray	1445:1454	arg1	data					1456:1459	Microarray data	1445:1459	Microarray data	1445:1459	Microarray data revealed numerous differentially expressed genes among the different treatment groups; here, we focused on studying the role of GPR18.
30308517	13	80	theme	SJC	1886:1888	arg1	composition					1871:1881	the chemical composition	1858:1881	the chemical composition of SJC	1858:1888	CONCLUSION Our data not only showed the chemical composition of SJC, but also demonstrated that verbenalin was a significant anti-inflammatory compound, which may function through GPR18.
30308517	11	81	theme	GPR18	1589:1593	arg1	role					1581:1584	the role	1577:1584	the role of GPR18	1577:1593	Microarray data revealed numerous differentially expressed genes among the different treatment groups; here, we focused on studying the role of GPR18.
30308517	4	82	theme	effects	639:645	arg1	mechanism					622:630	the mechanism	618:630	the mechanism of its effects in ARTI	618:653	In this study, we aimed to systematically explore its composition and the mechanism of its effects in ARTI.
30308517	4	82	theme	effects	639:645	arg1	composition					602:612	its composition	598:612	its composition	598:612	In this study, we aimed to systematically explore its composition and the mechanism of its effects in ARTI.
30308517	12	83	theme	PAK-induced	1798:1808	arg1	types					1815:1819	the untreated PAK-induced mice types	1784:1819	the untreated PAK-induced mice types	1784:1819	We found that the anti-inflammatory role of verbenalin was attenuated in GPR18 knockout mice compared with wild-type mice, although no statistically significant difference was observed in the untreated PAK-induced mice types.
30308517	4	84	from	mechanism	622:630	arg1	ARTI					650:653	ARTI	650:653	ARTI	650:653	In this study, we aimed to systematically explore its composition and the mechanism of its effects in ARTI.
31405147	0	0	theme	Mushroom	90:97	arg1	Polysaccharides					100:114	Yellow Strain Flammulina velutipes (Jinhua Mushroom) Polysaccharides	47:114	Yellow Strain Flammulina velutipes (Jinhua Mushroom) Polysaccharides	47:114	Characterization and Antioxidant Activities of Yellow Strain Flammulina velutipes (Jinhua Mushroom) Polysaccharides and Their Effects on ROS Content in L929 Cell.
31405147	2	1	from	study	396:400	arg1	functions					476:484	biological functions	465:484	biological functions	465:484	However, there has been little study on the physicochemical properties, antioxidant activities, and biological functions of yellow strain F. velutipes polysaccharides (FVYs).
31405147	2	1	from	study	396:400	arg1	properties					425:434	physicochemical properties	409:434	physicochemical properties	409:434	However, there has been little study on the physicochemical properties, antioxidant activities, and biological functions of yellow strain F. velutipes polysaccharides (FVYs).
31405147	2	1	from	study	396:400	arg1	activities					449:458	antioxidant activities	437:458	antioxidant activities	437:458	However, there has been little study on the physicochemical properties, antioxidant activities, and biological functions of yellow strain F. velutipes polysaccharides (FVYs).
31405147	1	2	from	mushroom	224:231	arg1	Taiwan					236:241	Taiwan	236:241	Taiwan	236:241	Yellow strain Flammulina velutipes, which is known as Jinhua mushroom in Taiwan, has become popular among customers due to its distinct texture that is utterly different from white strain F. velutipes.
31405147	3	3	theme	cosmetic	788:795	arg1	ingredient					797:806	cosmetic ingredient	788:806	cosmetic ingredient	788:806	The specific aims of this study are to evaluate and compare the physicochemical properties, antioxidant activities, and biological functions of FVYs and white strain F. velutipes polysaccharides (FVWs) in order to select the strain appropriate for cosmetic ingredient.
31405147	0	4	theme	Jinhua	83:88	arg1	Polysaccharides					100:114	Yellow Strain Flammulina velutipes (Jinhua Mushroom) Polysaccharides	47:114	Yellow Strain Flammulina velutipes (Jinhua Mushroom) Polysaccharides	47:114	Characterization and Antioxidant Activities of Yellow Strain Flammulina velutipes (Jinhua Mushroom) Polysaccharides and Their Effects on ROS Content in L929 Cell.
31405147	2	5	dep	properties	425:434	arg1	the					405:407	the	405:407	the	405:407	However, there has been little study on the physicochemical properties, antioxidant activities, and biological functions of yellow strain F. velutipes polysaccharides (FVYs).
31405147	7	6	theme	diacetate	1418:1426	arg1	assay					1438:1442	the dichloro-dihydro-fluorescein diacetate (DCFH-DA) assay	1385:1442	the dichloro-dihydro-fluorescein diacetate (DCFH-DA) assay	1385:1442	FVY-80 significantly reduced the reactive oxygen species (ROS) content in L929 cell by 55.96%, as compared with the H2O2-induced L929 cell, according to the dichloro-dihydro-fluorescein diacetate (DCFH-DA) assay.
31405147	5	7	theme	=	1118:1118	arg1	mg/mL					1125:1129	IC50 = 2.04 mg/mL	1113:1129	IC50 = 2.04 mg/mL	1113:1129	According to the results, FVY-80 showed the greatest antioxidant activities based on 2,2-diphenyl-1-picrylhydrazyl (DPPH) (IC50 = 2.22 mg/mL) and 2,2' -azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical assay (IC50 = 2.04 mg/mL).
31405147	5	7	theme	=	1118:1118	arg1	assay					1106:1110	2,2' -azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical assay	1036:1110	2,2' -azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical assay (IC50 = 2.04 mg/mL)	1036:1130	According to the results, FVY-80 showed the greatest antioxidant activities based on 2,2-diphenyl-1-picrylhydrazyl (DPPH) (IC50 = 2.22 mg/mL) and 2,2' -azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical assay (IC50 = 2.04 mg/mL).
31405147	0	8	theme	Polysaccharides	100:114	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization and Antioxidant Activities of Yellow Strain Flammulina velutipes (Jinhua Mushroom) Polysaccharides and Their Effects on ROS Content in L929 Cell.
31405147	0	8	theme	Polysaccharides	100:114	arg1	Activities					33:42	Antioxidant Activities	21:42	Antioxidant Activities	21:42	Characterization and Antioxidant Activities of Yellow Strain Flammulina velutipes (Jinhua Mushroom) Polysaccharides and Their Effects on ROS Content in L929 Cell.
31405147	0	9	from	Characterization	0:15	arg1	Content					141:147	ROS Content	137:147	ROS Content in L929 Cell	137:160	Characterization and Antioxidant Activities of Yellow Strain Flammulina velutipes (Jinhua Mushroom) Polysaccharides and Their Effects on ROS Content in L929 Cell.
31405147	4	10	dep	precipitation	855:867	arg1	%					886:886	80%	884:886	80%	884:886	The FVYs and FVWs were prepared by fractional precipitation (40%, 60%, and 80%).
31405147	4	10	dep	precipitation	855:867	arg1	%					872:872	40%	870:872	40%	870:872	The FVYs and FVWs were prepared by fractional precipitation (40%, 60%, and 80%).
31405147	4	10	dep	precipitation	855:867	arg1	%					877:877	60%	875:877	60%	875:877	The FVYs and FVWs were prepared by fractional precipitation (40%, 60%, and 80%).
31405147	7	11	theme	oxygen	1274:1279	arg1	species					1281:1287	reactive oxygen species	1265:1287	the reactive oxygen species (ROS) content in L929 cell	1261:1314	FVY-80 significantly reduced the reactive oxygen species (ROS) content in L929 cell by 55.96%, as compared with the H2O2-induced L929 cell, according to the dichloro-dihydro-fluorescein diacetate (DCFH-DA) assay.
31405147	7	11	theme	oxygen	1274:1279	arg1	ROS					1290:1292	ROS	1290:1292	ROS	1290:1292	FVY-80 significantly reduced the reactive oxygen species (ROS) content in L929 cell by 55.96%, as compared with the H2O2-induced L929 cell, according to the dichloro-dihydro-fluorescein diacetate (DCFH-DA) assay.
31405147	3	12	theme	appropriate	772:782	arg1	strain					765:770	the strain	761:770	the strain appropriate for cosmetic ingredient	761:806	The specific aims of this study are to evaluate and compare the physicochemical properties, antioxidant activities, and biological functions of FVYs and white strain F. velutipes polysaccharides (FVWs) in order to select the strain appropriate for cosmetic ingredient.
31405147	2	13	theme	strain	496:501	arg1	FVYs					533:536	FVYs	533:536	FVYs	533:536	However, there has been little study on the physicochemical properties, antioxidant activities, and biological functions of yellow strain F. velutipes polysaccharides (FVYs).
31405147	2	13	theme	strain	496:501	arg1	polysaccharides					516:530	yellow strain F. velutipes polysaccharides	489:530	yellow strain F. velutipes polysaccharides (FVYs)	489:537	However, there has been little study on the physicochemical properties, antioxidant activities, and biological functions of yellow strain F. velutipes polysaccharides (FVYs).
31405147	7	14	theme	L929	1361:1364	arg1	cell					1366:1369	the H2O2-induced L929 cell	1344:1369	the H2O2-induced L929 cell	1344:1369	FVY-80 significantly reduced the reactive oxygen species (ROS) content in L929 cell by 55.96%, as compared with the H2O2-induced L929 cell, according to the dichloro-dihydro-fluorescein diacetate (DCFH-DA) assay.
31405147	1	15	from	velutipes	354:362	arg1	different					323:331	different	323:331	different	323:331	Yellow strain Flammulina velutipes, which is known as Jinhua mushroom in Taiwan, has become popular among customers due to its distinct texture that is utterly different from white strain F. velutipes.
31405147	1	16	theme	white	338:342	arg1	velutipes					354:362	white strain F. velutipes	338:362	white strain F. velutipes	338:362	Yellow strain Flammulina velutipes, which is known as Jinhua mushroom in Taiwan, has become popular among customers due to its distinct texture that is utterly different from white strain F. velutipes.
31405147	7	17	theme	L929	1306:1309	arg1	cell					1311:1314	L929 cell	1306:1314	L929 cell	1306:1314	FVY-80 significantly reduced the reactive oxygen species (ROS) content in L929 cell by 55.96%, as compared with the H2O2-induced L929 cell, according to the dichloro-dihydro-fluorescein diacetate (DCFH-DA) assay.
31405147	7	18	theme	species	1281:1287	arg1	content					1295:1301	the reactive oxygen species (ROS) content	1261:1301	the reactive oxygen species (ROS) content in L929 cell	1261:1314	FVY-80 significantly reduced the reactive oxygen species (ROS) content in L929 cell by 55.96%, as compared with the H2O2-induced L929 cell, according to the dichloro-dihydro-fluorescein diacetate (DCFH-DA) assay.
31405147	1	19	theme	Yellow	163:168	arg1	mushroom					224:231	Jinhua mushroom	217:231	Jinhua mushroom in Taiwan	217:241	Yellow strain Flammulina velutipes, which is known as Jinhua mushroom in Taiwan, has become popular among customers due to its distinct texture that is utterly different from white strain F. velutipes.
31405147	1	19	theme	Yellow	163:168	arg1	strain					170:175	Yellow strain Flammulina velutipes	163:196	Yellow strain Flammulina velutipes	163:196	Yellow strain Flammulina velutipes, which is known as Jinhua mushroom in Taiwan, has become popular among customers due to its distinct texture that is utterly different from white strain F. velutipes.
31405147	1	20	theme	strain	344:349	arg1	velutipes					354:362	white strain F. velutipes	338:362	white strain F. velutipes	338:362	Yellow strain Flammulina velutipes, which is known as Jinhua mushroom in Taiwan, has become popular among customers due to its distinct texture that is utterly different from white strain F. velutipes.
31405147	5	21	theme	IC50	1113:1116	arg1	mg/mL					1125:1129	IC50 = 2.04 mg/mL	1113:1129	IC50 = 2.04 mg/mL	1113:1129	According to the results, FVY-80 showed the greatest antioxidant activities based on 2,2-diphenyl-1-picrylhydrazyl (DPPH) (IC50 = 2.22 mg/mL) and 2,2' -azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical assay (IC50 = 2.04 mg/mL).
31405147	5	21	theme	IC50	1113:1116	arg1	assay					1106:1110	2,2' -azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical assay	1036:1110	2,2' -azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical assay (IC50 = 2.04 mg/mL)	1036:1130	According to the results, FVY-80 showed the greatest antioxidant activities based on 2,2-diphenyl-1-picrylhydrazyl (DPPH) (IC50 = 2.22 mg/mL) and 2,2' -azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical assay (IC50 = 2.04 mg/mL).
31405147	2	22	theme	yellow	489:494	arg1	FVYs					533:536	FVYs	533:536	FVYs	533:536	However, there has been little study on the physicochemical properties, antioxidant activities, and biological functions of yellow strain F. velutipes polysaccharides (FVYs).
31405147	2	22	theme	yellow	489:494	arg1	polysaccharides					516:530	yellow strain F. velutipes polysaccharides	489:530	yellow strain F. velutipes polysaccharides (FVYs)	489:537	However, there has been little study on the physicochemical properties, antioxidant activities, and biological functions of yellow strain F. velutipes polysaccharides (FVYs).
31405147	2	23	theme	physicochemical	409:423	arg1	properties					425:434	physicochemical properties	409:434	physicochemical properties	409:434	However, there has been little study on the physicochemical properties, antioxidant activities, and biological functions of yellow strain F. velutipes polysaccharides (FVYs).
31405147	1	24	theme	F.	351:352	arg1	velutipes					354:362	white strain F. velutipes	338:362	white strain F. velutipes	338:362	Yellow strain Flammulina velutipes, which is known as Jinhua mushroom in Taiwan, has become popular among customers due to its distinct texture that is utterly different from white strain F. velutipes.
31405147	6	25	theme	fractions	1145:1153	arg1	None					1133:1136	None	1133:1136	None of the fractions	1133:1153	None of the fractions exhibited cytotoxicity toward L929 cell under a concentration of 500 μ g/mL.
31405147	5	26	theme	=	1018:1018	arg1	IC50					1013:1016	IC50	1013:1016	IC50 = 2.22 mg/mL	1013:1029	According to the results, FVY-80 showed the greatest antioxidant activities based on 2,2-diphenyl-1-picrylhydrazyl (DPPH) (IC50 = 2.22 mg/mL) and 2,2' -azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical assay (IC50 = 2.04 mg/mL).
31405147	1	27	theme	Flammulina	177:186	arg1	velutipes					188:196	Flammulina velutipes	177:196	Yellow strain Flammulina velutipes	163:196	Yellow strain Flammulina velutipes, which is known as Jinhua mushroom in Taiwan, has become popular among customers due to its distinct texture that is utterly different from white strain F. velutipes.
31405147	6	28	theme	g/mL	1226:1229	arg1	concentration					1203:1215	a concentration	1201:1215	a concentration of 500 μ g/mL	1201:1229	None of the fractions exhibited cytotoxicity toward L929 cell under a concentration of 500 μ g/mL.
31405147	3	29	theme	polysaccharides	719:733	arg1	functions					671:679	biological functions	660:679	biological functions	660:679	The specific aims of this study are to evaluate and compare the physicochemical properties, antioxidant activities, and biological functions of FVYs and white strain F. velutipes polysaccharides (FVWs) in order to select the strain appropriate for cosmetic ingredient.
31405147	3	29	theme	polysaccharides	719:733	arg1	properties					620:629	the physicochemical properties	600:629	the physicochemical properties	600:629	The specific aims of this study are to evaluate and compare the physicochemical properties, antioxidant activities, and biological functions of FVYs and white strain F. velutipes polysaccharides (FVWs) in order to select the strain appropriate for cosmetic ingredient.
31405147	3	29	theme	polysaccharides	719:733	arg1	activities					644:653	antioxidant activities	632:653	antioxidant activities	632:653	The specific aims of this study are to evaluate and compare the physicochemical properties, antioxidant activities, and biological functions of FVYs and white strain F. velutipes polysaccharides (FVWs) in order to select the strain appropriate for cosmetic ingredient.
31405147	3	30	theme	specific	544:551	arg1	aims					553:556	The specific aims	540:556	The specific aims of this study	540:570	The specific aims of this study are to evaluate and compare the physicochemical properties, antioxidant activities, and biological functions of FVYs and white strain F. velutipes polysaccharides (FVWs) in order to select the strain appropriate for cosmetic ingredient.
31405147	5	31	theme	radical	1098:1104	arg1	mg/mL					1125:1129	IC50 = 2.04 mg/mL	1113:1129	IC50 = 2.04 mg/mL	1113:1129	According to the results, FVY-80 showed the greatest antioxidant activities based on 2,2-diphenyl-1-picrylhydrazyl (DPPH) (IC50 = 2.22 mg/mL) and 2,2' -azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical assay (IC50 = 2.04 mg/mL).
31405147	5	31	theme	radical	1098:1104	arg1	assay					1106:1110	2,2' -azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical assay	1036:1110	2,2' -azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical assay (IC50 = 2.04 mg/mL)	1036:1130	According to the results, FVY-80 showed the greatest antioxidant activities based on 2,2-diphenyl-1-picrylhydrazyl (DPPH) (IC50 = 2.22 mg/mL) and 2,2' -azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical assay (IC50 = 2.04 mg/mL).
31405147	5	32	theme	antioxidant	943:953	arg1	activities					955:964	the greatest antioxidant activities	930:964	the greatest antioxidant activities based on 2,2-diphenyl-1-picrylhydrazyl (DPPH) (IC50 = 2.22 mg/mL) and 2,2' -azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical assay (IC50 = 2.04 mg/mL)	930:1130	According to the results, FVY-80 showed the greatest antioxidant activities based on 2,2-diphenyl-1-picrylhydrazyl (DPPH) (IC50 = 2.22 mg/mL) and 2,2' -azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical assay (IC50 = 2.04 mg/mL).
31405147	2	33	theme	little	389:394	arg1	study					396:400	little study	389:400	little study on the physicochemical properties, antioxidant activities, and biological functions of yellow strain F. velutipes polysaccharides (FVYs)	389:537	However, there has been little study on the physicochemical properties, antioxidant activities, and biological functions of yellow strain F. velutipes polysaccharides (FVYs).
31405147	0	34	theme	Antioxidant	21:31	arg1	Activities					33:42	Antioxidant Activities	21:42	Antioxidant Activities	21:42	Characterization and Antioxidant Activities of Yellow Strain Flammulina velutipes (Jinhua Mushroom) Polysaccharides and Their Effects on ROS Content in L929 Cell.
31405147	3	35	theme	F.	706:707	arg1	polysaccharides					719:733	FVYs and white strain F. velutipes polysaccharides	684:733	FVYs and white strain F. velutipes polysaccharides (FVWs)	684:740	The specific aims of this study are to evaluate and compare the physicochemical properties, antioxidant activities, and biological functions of FVYs and white strain F. velutipes polysaccharides (FVWs) in order to select the strain appropriate for cosmetic ingredient.
31405147	3	35	theme	F.	706:707	arg1	FVWs					736:739	FVWs	736:739	FVWs	736:739	The specific aims of this study are to evaluate and compare the physicochemical properties, antioxidant activities, and biological functions of FVYs and white strain F. velutipes polysaccharides (FVWs) in order to select the strain appropriate for cosmetic ingredient.
31405147	0	36	theme	Effects	126:132	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization and Antioxidant Activities of Yellow Strain Flammulina velutipes (Jinhua Mushroom) Polysaccharides and Their Effects on ROS Content in L929 Cell.
31405147	0	36	theme	Effects	126:132	arg1	Activities					33:42	Antioxidant Activities	21:42	Antioxidant Activities	21:42	Characterization and Antioxidant Activities of Yellow Strain Flammulina velutipes (Jinhua Mushroom) Polysaccharides and Their Effects on ROS Content in L929 Cell.
31405147	3	37	theme	physicochemical	604:618	arg1	properties					620:629	the physicochemical properties	600:629	the physicochemical properties	600:629	The specific aims of this study are to evaluate and compare the physicochemical properties, antioxidant activities, and biological functions of FVYs and white strain F. velutipes polysaccharides (FVWs) in order to select the strain appropriate for cosmetic ingredient.
31405147	0	38	theme	Yellow	47:52	arg1	Polysaccharides					100:114	Yellow Strain Flammulina velutipes (Jinhua Mushroom) Polysaccharides	47:114	Yellow Strain Flammulina velutipes (Jinhua Mushroom) Polysaccharides	47:114	Characterization and Antioxidant Activities of Yellow Strain Flammulina velutipes (Jinhua Mushroom) Polysaccharides and Their Effects on ROS Content in L929 Cell.
31405147	1	39	theme	due	279:281	arg1	customers					269:277	customers	269:277	customers due to its distinct texture that is utterly different from white strain F. velutipes	269:362	Yellow strain Flammulina velutipes, which is known as Jinhua mushroom in Taiwan, has become popular among customers due to its distinct texture that is utterly different from white strain F. velutipes.
31405147	2	40	theme	polysaccharides	516:530	arg1	functions					476:484	biological functions	465:484	biological functions	465:484	However, there has been little study on the physicochemical properties, antioxidant activities, and biological functions of yellow strain F. velutipes polysaccharides (FVYs).
31405147	2	40	theme	polysaccharides	516:530	arg1	properties					425:434	physicochemical properties	409:434	physicochemical properties	409:434	However, there has been little study on the physicochemical properties, antioxidant activities, and biological functions of yellow strain F. velutipes polysaccharides (FVYs).
31405147	2	40	theme	polysaccharides	516:530	arg1	activities					449:458	antioxidant activities	437:458	antioxidant activities	437:458	However, there has been little study on the physicochemical properties, antioxidant activities, and biological functions of yellow strain F. velutipes polysaccharides (FVYs).
31405147	7	41	theme	DCFH-DA	1429:1435	arg1	assay					1438:1442	the dichloro-dihydro-fluorescein diacetate (DCFH-DA) assay	1385:1442	the dichloro-dihydro-fluorescein diacetate (DCFH-DA) assay	1385:1442	FVY-80 significantly reduced the reactive oxygen species (ROS) content in L929 cell by 55.96%, as compared with the H2O2-induced L929 cell, according to the dichloro-dihydro-fluorescein diacetate (DCFH-DA) assay.
31405147	2	42	theme	antioxidant	437:447	arg1	activities					449:458	antioxidant activities	437:458	antioxidant activities	437:458	However, there has been little study on the physicochemical properties, antioxidant activities, and biological functions of yellow strain F. velutipes polysaccharides (FVYs).
31405147	3	43	dep	FVYs	684:687	arg1	velutipes					709:717	velutipes	709:717	velutipes	709:717	The specific aims of this study are to evaluate and compare the physicochemical properties, antioxidant activities, and biological functions of FVYs and white strain F. velutipes polysaccharides (FVWs) in order to select the strain appropriate for cosmetic ingredient.
31405147	0	44	theme	ROS	137:139	arg1	Content					141:147	ROS Content	137:147	ROS Content in L929 Cell	137:160	Characterization and Antioxidant Activities of Yellow Strain Flammulina velutipes (Jinhua Mushroom) Polysaccharides and Their Effects on ROS Content in L929 Cell.
31405147	7	45	theme	dichloro-dihydro-fluorescein	1389:1416	arg1	assay					1438:1442	the dichloro-dihydro-fluorescein diacetate (DCFH-DA) assay	1385:1442	the dichloro-dihydro-fluorescein diacetate (DCFH-DA) assay	1385:1442	FVY-80 significantly reduced the reactive oxygen species (ROS) content in L929 cell by 55.96%, as compared with the H2O2-induced L929 cell, according to the dichloro-dihydro-fluorescein diacetate (DCFH-DA) assay.
31405147	3	46	theme	study	566:570	arg1	aims					553:556	The specific aims	540:556	The specific aims of this study	540:570	The specific aims of this study are to evaluate and compare the physicochemical properties, antioxidant activities, and biological functions of FVYs and white strain F. velutipes polysaccharides (FVWs) in order to select the strain appropriate for cosmetic ingredient.
31405147	2	47	theme	velutipes	506:514	arg1	FVYs					533:536	FVYs	533:536	FVYs	533:536	However, there has been little study on the physicochemical properties, antioxidant activities, and biological functions of yellow strain F. velutipes polysaccharides (FVYs).
31405147	2	47	theme	velutipes	506:514	arg1	polysaccharides					516:530	yellow strain F. velutipes polysaccharides	489:530	yellow strain F. velutipes polysaccharides (FVYs)	489:537	However, there has been little study on the physicochemical properties, antioxidant activities, and biological functions of yellow strain F. velutipes polysaccharides (FVYs).
31405147	5	48	dep	=	1018:1018	arg1	mg/mL					1025:1029	2.22 mg/mL	1020:1029	2.22 mg/mL	1020:1029	According to the results, FVY-80 showed the greatest antioxidant activities based on 2,2-diphenyl-1-picrylhydrazyl (DPPH) (IC50 = 2.22 mg/mL) and 2,2' -azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical assay (IC50 = 2.04 mg/mL).
31405147	0	49	theme	Flammulina	61:70	arg1	Polysaccharides					100:114	Yellow Strain Flammulina velutipes (Jinhua Mushroom) Polysaccharides	47:114	Yellow Strain Flammulina velutipes (Jinhua Mushroom) Polysaccharides	47:114	Characterization and Antioxidant Activities of Yellow Strain Flammulina velutipes (Jinhua Mushroom) Polysaccharides and Their Effects on ROS Content in L929 Cell.
31405147	2	50	theme	F.	503:504	arg1	FVYs					533:536	FVYs	533:536	FVYs	533:536	However, there has been little study on the physicochemical properties, antioxidant activities, and biological functions of yellow strain F. velutipes polysaccharides (FVYs).
31405147	2	50	theme	F.	503:504	arg1	polysaccharides					516:530	yellow strain F. velutipes polysaccharides	489:530	yellow strain F. velutipes polysaccharides (FVYs)	489:537	However, there has been little study on the physicochemical properties, antioxidant activities, and biological functions of yellow strain F. velutipes polysaccharides (FVYs).
31405147	0	51	theme	L929	152:155	arg1	Cell					157:160	L929 Cell	152:160	L929 Cell	152:160	Characterization and Antioxidant Activities of Yellow Strain Flammulina velutipes (Jinhua Mushroom) Polysaccharides and Their Effects on ROS Content in L929 Cell.
31405147	2	52	theme	biological	465:474	arg1	functions					476:484	biological functions	465:484	biological functions	465:484	However, there has been little study on the physicochemical properties, antioxidant activities, and biological functions of yellow strain F. velutipes polysaccharides (FVYs).
31405147	5	53	theme	greatest	934:941	arg1	activities					955:964	the greatest antioxidant activities	930:964	the greatest antioxidant activities based on 2,2-diphenyl-1-picrylhydrazyl (DPPH) (IC50 = 2.22 mg/mL) and 2,2' -azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical assay (IC50 = 2.04 mg/mL)	930:1130	According to the results, FVY-80 showed the greatest antioxidant activities based on 2,2-diphenyl-1-picrylhydrazyl (DPPH) (IC50 = 2.22 mg/mL) and 2,2' -azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical assay (IC50 = 2.04 mg/mL).
31405147	3	54	theme	FVYs	684:687	arg1	polysaccharides					719:733	FVYs and white strain F. velutipes polysaccharides	684:733	FVYs and white strain F. velutipes polysaccharides (FVWs)	684:740	The specific aims of this study are to evaluate and compare the physicochemical properties, antioxidant activities, and biological functions of FVYs and white strain F. velutipes polysaccharides (FVWs) in order to select the strain appropriate for cosmetic ingredient.
31405147	3	54	theme	FVYs	684:687	arg1	FVWs					736:739	FVWs	736:739	FVWs	736:739	The specific aims of this study are to evaluate and compare the physicochemical properties, antioxidant activities, and biological functions of FVYs and white strain F. velutipes polysaccharides (FVWs) in order to select the strain appropriate for cosmetic ingredient.
31405147	0	55	theme	Strain	54:59	arg1	Polysaccharides					100:114	Yellow Strain Flammulina velutipes (Jinhua Mushroom) Polysaccharides	47:114	Yellow Strain Flammulina velutipes (Jinhua Mushroom) Polysaccharides	47:114	Characterization and Antioxidant Activities of Yellow Strain Flammulina velutipes (Jinhua Mushroom) Polysaccharides and Their Effects on ROS Content in L929 Cell.
31405147	1	56	theme	distinct	290:297	arg1	texture					299:305	its distinct texture	286:305	its distinct texture that is utterly different from white strain F. velutipes	286:362	Yellow strain Flammulina velutipes, which is known as Jinhua mushroom in Taiwan, has become popular among customers due to its distinct texture that is utterly different from white strain F. velutipes.
31405147	4	57	theme	fractional	844:853	arg1	precipitation					855:867	fractional precipitation	844:867	fractional precipitation (40%, 60%, and 80%)	844:887	The FVYs and FVWs were prepared by fractional precipitation (40%, 60%, and 80%).
31405147	1	58	dep	strain	170:175	arg1	velutipes					188:196	Flammulina velutipes	177:196	Yellow strain Flammulina velutipes	163:196	Yellow strain Flammulina velutipes, which is known as Jinhua mushroom in Taiwan, has become popular among customers due to its distinct texture that is utterly different from white strain F. velutipes.
31405147	5	59	theme	-azino-bis-3-ethylbenzthiazoline-6-sulphonic	1041:1084	arg1	mg/mL					1125:1129	IC50 = 2.04 mg/mL	1113:1129	IC50 = 2.04 mg/mL	1113:1129	According to the results, FVY-80 showed the greatest antioxidant activities based on 2,2-diphenyl-1-picrylhydrazyl (DPPH) (IC50 = 2.22 mg/mL) and 2,2' -azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical assay (IC50 = 2.04 mg/mL).
31405147	5	59	theme	-azino-bis-3-ethylbenzthiazoline-6-sulphonic	1041:1084	arg1	assay					1106:1110	2,2' -azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical assay	1036:1110	2,2' -azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical assay (IC50 = 2.04 mg/mL)	1036:1130	According to the results, FVY-80 showed the greatest antioxidant activities based on 2,2-diphenyl-1-picrylhydrazyl (DPPH) (IC50 = 2.22 mg/mL) and 2,2' -azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical assay (IC50 = 2.04 mg/mL).
31405147	6	60	theme	L929	1185:1188	arg1	cell					1190:1193	L929 cell	1185:1193	L929 cell	1185:1193	None of the fractions exhibited cytotoxicity toward L929 cell under a concentration of 500 μ g/mL.
31405147	7	61	theme	H2O2-induced	1348:1359	arg1	cell					1366:1369	the H2O2-induced L929 cell	1344:1369	the H2O2-induced L929 cell	1344:1369	FVY-80 significantly reduced the reactive oxygen species (ROS) content in L929 cell by 55.96%, as compared with the H2O2-induced L929 cell, according to the dichloro-dihydro-fluorescein diacetate (DCFH-DA) assay.
31405147	5	62	theme	ABTS	1092:1095	arg1	mg/mL					1125:1129	IC50 = 2.04 mg/mL	1113:1129	IC50 = 2.04 mg/mL	1113:1129	According to the results, FVY-80 showed the greatest antioxidant activities based on 2,2-diphenyl-1-picrylhydrazyl (DPPH) (IC50 = 2.22 mg/mL) and 2,2' -azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical assay (IC50 = 2.04 mg/mL).
31405147	5	62	theme	ABTS	1092:1095	arg1	assay					1106:1110	2,2' -azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical assay	1036:1110	2,2' -azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical assay (IC50 = 2.04 mg/mL)	1036:1130	According to the results, FVY-80 showed the greatest antioxidant activities based on 2,2-diphenyl-1-picrylhydrazyl (DPPH) (IC50 = 2.22 mg/mL) and 2,2' -azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical assay (IC50 = 2.04 mg/mL).
31405147	5	63	dep	2,2-diphenyl-1-picrylhydrazyl	975:1003	arg1	IC50					1013:1016	IC50	1013:1016	IC50 = 2.22 mg/mL	1013:1029	According to the results, FVY-80 showed the greatest antioxidant activities based on 2,2-diphenyl-1-picrylhydrazyl (DPPH) (IC50 = 2.22 mg/mL) and 2,2' -azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical assay (IC50 = 2.04 mg/mL).
31405147	6	64	theme	μ	1224:1224	arg1	g/mL					1226:1229	500 μ g/mL	1220:1229	500 μ g/mL	1220:1229	None of the fractions exhibited cytotoxicity toward L929 cell under a concentration of 500 μ g/mL.
31405147	7	65	from	content	1295:1301	arg1	cell					1311:1314	L929 cell	1306:1314	L929 cell	1306:1314	FVY-80 significantly reduced the reactive oxygen species (ROS) content in L929 cell by 55.96%, as compared with the H2O2-induced L929 cell, according to the dichloro-dihydro-fluorescein diacetate (DCFH-DA) assay.
31405147	0	66	from	Content	141:147	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization and Antioxidant Activities of Yellow Strain Flammulina velutipes (Jinhua Mushroom) Polysaccharides and Their Effects on ROS Content in L929 Cell.
31405147	0	66	from	Content	141:147	arg1	Activities					33:42	Antioxidant Activities	21:42	Antioxidant Activities	21:42	Characterization and Antioxidant Activities of Yellow Strain Flammulina velutipes (Jinhua Mushroom) Polysaccharides and Their Effects on ROS Content in L929 Cell.
31405147	0	66	from	Content	141:147	arg1	Cell					157:160	L929 Cell	152:160	L929 Cell	152:160	Characterization and Antioxidant Activities of Yellow Strain Flammulina velutipes (Jinhua Mushroom) Polysaccharides and Their Effects on ROS Content in L929 Cell.
31405147	0	67	from	Polysaccharides	100:114	arg1	Content					141:147	ROS Content	137:147	ROS Content in L929 Cell	137:160	Characterization and Antioxidant Activities of Yellow Strain Flammulina velutipes (Jinhua Mushroom) Polysaccharides and Their Effects on ROS Content in L929 Cell.
31405147	7	68	theme	reactive	1265:1272	arg1	species					1281:1287	reactive oxygen species	1265:1287	the reactive oxygen species (ROS) content in L929 cell	1261:1314	FVY-80 significantly reduced the reactive oxygen species (ROS) content in L929 cell by 55.96%, as compared with the H2O2-induced L929 cell, according to the dichloro-dihydro-fluorescein diacetate (DCFH-DA) assay.
31405147	7	68	theme	reactive	1265:1272	arg1	ROS					1290:1292	ROS	1290:1292	ROS	1290:1292	FVY-80 significantly reduced the reactive oxygen species (ROS) content in L929 cell by 55.96%, as compared with the H2O2-induced L929 cell, according to the dichloro-dihydro-fluorescein diacetate (DCFH-DA) assay.
31405147	1	69	theme	Jinhua	217:222	arg1	mushroom					224:231	Jinhua mushroom	217:231	Jinhua mushroom in Taiwan	217:241	Yellow strain Flammulina velutipes, which is known as Jinhua mushroom in Taiwan, has become popular among customers due to its distinct texture that is utterly different from white strain F. velutipes.
31405147	1	69	theme	Jinhua	217:222	arg1	strain					170:175	Yellow strain Flammulina velutipes	163:196	Yellow strain Flammulina velutipes	163:196	Yellow strain Flammulina velutipes, which is known as Jinhua mushroom in Taiwan, has become popular among customers due to its distinct texture that is utterly different from white strain F. velutipes.
31405147	5	70	theme	acid	1086:1089	arg1	mg/mL					1125:1129	IC50 = 2.04 mg/mL	1113:1129	IC50 = 2.04 mg/mL	1113:1129	According to the results, FVY-80 showed the greatest antioxidant activities based on 2,2-diphenyl-1-picrylhydrazyl (DPPH) (IC50 = 2.22 mg/mL) and 2,2' -azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical assay (IC50 = 2.04 mg/mL).
31405147	5	70	theme	acid	1086:1089	arg1	assay					1106:1110	2,2' -azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical assay	1036:1110	2,2' -azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical assay (IC50 = 2.04 mg/mL)	1036:1130	According to the results, FVY-80 showed the greatest antioxidant activities based on 2,2-diphenyl-1-picrylhydrazyl (DPPH) (IC50 = 2.22 mg/mL) and 2,2' -azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical assay (IC50 = 2.04 mg/mL).
31405147	3	71	theme	antioxidant	632:642	arg1	activities					644:653	antioxidant activities	632:653	antioxidant activities	632:653	The specific aims of this study are to evaluate and compare the physicochemical properties, antioxidant activities, and biological functions of FVYs and white strain F. velutipes polysaccharides (FVWs) in order to select the strain appropriate for cosmetic ingredient.
31405147	3	72	theme	biological	660:669	arg1	functions					671:679	biological functions	660:679	biological functions	660:679	The specific aims of this study are to evaluate and compare the physicochemical properties, antioxidant activities, and biological functions of FVYs and white strain F. velutipes polysaccharides (FVWs) in order to select the strain appropriate for cosmetic ingredient.
31405147	8	73	theme	best	1490:1493	arg1	source					1495:1500	the best source	1486:1500	the best source for a cosmetics ingredient	1486:1527	In conclusion, we suggest that FVY-80 is the best source for a cosmetics ingredient.
31405147	8	73	theme	best	1490:1493	arg1	FVY-80					1476:1481	FVY-80	1476:1481	FVY-80	1476:1481	In conclusion, we suggest that FVY-80 is the best source for a cosmetics ingredient.
31405147	8	74	theme	cosmetics	1508:1516	arg1	ingredient					1518:1527	a cosmetics ingredient	1506:1527	a cosmetics ingredient	1506:1527	In conclusion, we suggest that FVY-80 is the best source for a cosmetics ingredient.
31405147	0	75	theme	velutipes	72:80	arg1	Polysaccharides					100:114	Yellow Strain Flammulina velutipes (Jinhua Mushroom) Polysaccharides	47:114	Yellow Strain Flammulina velutipes (Jinhua Mushroom) Polysaccharides	47:114	Characterization and Antioxidant Activities of Yellow Strain Flammulina velutipes (Jinhua Mushroom) Polysaccharides and Their Effects on ROS Content in L929 Cell.
31405147	0	76	from	Activities	33:42	arg1	Content					141:147	ROS Content	137:147	ROS Content in L929 Cell	137:160	Characterization and Antioxidant Activities of Yellow Strain Flammulina velutipes (Jinhua Mushroom) Polysaccharides and Their Effects on ROS Content in L929 Cell.
31405147	0	77	from	Effects	126:132	arg1	Content					141:147	ROS Content	137:147	ROS Content in L929 Cell	137:160	Characterization and Antioxidant Activities of Yellow Strain Flammulina velutipes (Jinhua Mushroom) Polysaccharides and Their Effects on ROS Content in L929 Cell.
30625594	0	0	theme	polysaccharides	76:90	arg1	effect					66:71	the synergistic effect	50:71	the synergistic effect of polysaccharides for the recognition of tyrosine enantiomers	50:134	A novel electrochemical chiral interface based on the synergistic effect of polysaccharides for the recognition of tyrosine enantiomers.
30625594	4	1	theme	peak	768:771	arg1	current					773:779	peak current	768:779	peak current	768:779	In addition, a good linear relationship between Tyr enantiomer concentration and peak current could be observed.
30625594	7	2	theme	substances	1305:1314	arg1	concentrations					1273:1286	10-fold higher concentrations	1258:1286	10-fold higher concentrations of physiological substances and various metal compounds	1258:1342	The proposed SS-CS/GCE-based chiral sensor exhibited high anti-interference ability in the presence of 10-fold higher concentrations of physiological substances and various metal compounds.
30625594	2	3	theme	Brunauer-Emmett-Teller	431:452	arg1	method					460:465	Brunauer-Emmett-Teller (BET) method	431:465	Brunauer-Emmett-Teller (BET) method	431:465	The SS-CS composite was characterized by scanning electron microscopy (SEM) and Brunauer-Emmett-Teller (BET) method.
30625594	1	4	theme	enantiomers	304:314	arg1	recognition					274:284	chiral recognition	267:284	chiral recognition of tyrosine (Tyr) enantiomers	267:314	Electrochemical chiral interface based on two polysaccharides, soluble starch (SS) and chitosan (CS), was fabricated and used for chiral recognition of tyrosine (Tyr) enantiomers via square wave voltammetry (SWV).
30625594	6	5	from	ratios	1015:1020	arg1	mixture					1051:1057	a racemic mixture	1041:1057	a racemic mixture	1041:1057	The novel SS-CS/GCE showed an ability to predict the ratios of L- and D-Tyr in a racemic mixture and exhibited the possibility of qualitative and quantitative determination of Tyr enantiomers.
30625594	5	6	dep	determination	845:857	arg1	0.99631					870:876	R2D-Tyr = 0.99631 and R2L-Tyr = 0.98333	860:898	0.99631	870:876	The SS-CS/GCE exhibited good coefficients of determination (R2D-Tyr = 0.99631 and R2L-Tyr = 0.98333) for D-Tyr and L-Tyr in the concentration range 0.01-1.00 mM.
30625594	5	6	dep	determination	845:857	arg1	0.98333					892:898	R2D-Tyr = 0.99631 and R2L-Tyr = 0.98333	860:898	0.98333	892:898	The SS-CS/GCE exhibited good coefficients of determination (R2D-Tyr = 0.99631 and R2L-Tyr = 0.98333) for D-Tyr and L-Tyr in the concentration range 0.01-1.00 mM.
30625594	8	7	contain	possessed	1370:1378	arg2	reproducibility					1413:1427	excellent reproducibility	1403:1427	excellent reproducibility	1403:1427	Meanwhile, the SS-CS/GCE possessed good repeatability and excellent reproducibility.
30625594	8	7	contain	possessed	1370:1378	arg1	SS-CS/GCE					1360:1368	the SS-CS/GCE	1356:1368	the SS-CS/GCE	1356:1368	Meanwhile, the SS-CS/GCE possessed good repeatability and excellent reproducibility.
30625594	8	7	contain	possessed	1370:1378	arg1	Meanwhile					1345:1353	Meanwhile	1345:1353	Meanwhile	1345:1353	Meanwhile, the SS-CS/GCE possessed good repeatability and excellent reproducibility.
30625594	8	7	contain	possessed	1370:1378	arg2	repeatability					1385:1397	good repeatability	1380:1397	good repeatability	1380:1397	Meanwhile, the SS-CS/GCE possessed good repeatability and excellent reproducibility.
30625594	3	8	theme	=	623:623	arg1	potential					608:616	the peak potential	599:616	the peak potential (ΔEp = ED - EL)	599:632	Under the optimized experimental conditions, the oxidation peak current ratio of L-Tyr to D-Tyr (IL/ID) and the difference between the peak potential (ΔEp = ED - EL) were observed to be 1.38 and 12 mV at the SS-CS/GCE.
30625594	3	8	theme	=	623:623	arg1	EL					630:631	ΔEp = ED - EL	619:631	ΔEp = ED - EL	619:631	Under the optimized experimental conditions, the oxidation peak current ratio of L-Tyr to D-Tyr (IL/ID) and the difference between the peak potential (ΔEp = ED - EL) were observed to be 1.38 and 12 mV at the SS-CS/GCE.
30625594	5	9	theme	=	868:868	arg1	0.99631					870:876	R2D-Tyr = 0.99631 and R2L-Tyr = 0.98333	860:898	0.99631	870:876	The SS-CS/GCE exhibited good coefficients of determination (R2D-Tyr = 0.99631 and R2L-Tyr = 0.98333) for D-Tyr and L-Tyr in the concentration range 0.01-1.00 mM.
30625594	5	10	theme	R2D-Tyr	860:866	arg1	0.99631					870:876	R2D-Tyr = 0.99631 and R2L-Tyr = 0.98333	860:898	0.99631	870:876	The SS-CS/GCE exhibited good coefficients of determination (R2D-Tyr = 0.99631 and R2L-Tyr = 0.98333) for D-Tyr and L-Tyr in the concentration range 0.01-1.00 mM.
30625594	6	11	theme	determination	1121:1133	arg1	possibility					1077:1087	the possibility	1073:1087	the possibility of qualitative and quantitative determination of Tyr enantiomers	1073:1152	The novel SS-CS/GCE showed an ability to predict the ratios of L- and D-Tyr in a racemic mixture and exhibited the possibility of qualitative and quantitative determination of Tyr enantiomers.
30625594	5	12	theme	determination	845:857	arg1	coefficients					829:840	good coefficients	824:840	good coefficients of determination (R2D-Tyr = 0.99631 and R2L-Tyr = 0.98333) for D-Tyr and L-Tyr	824:919	The SS-CS/GCE exhibited good coefficients of determination (R2D-Tyr = 0.99631 and R2L-Tyr = 0.98333) for D-Tyr and L-Tyr in the concentration range 0.01-1.00 mM.
30625594	1	13	theme	square	320:325	arg1	SWV					345:347	SWV	345:347	SWV	345:347	Electrochemical chiral interface based on two polysaccharides, soluble starch (SS) and chitosan (CS), was fabricated and used for chiral recognition of tyrosine (Tyr) enantiomers via square wave voltammetry (SWV).
30625594	1	13	theme	square	320:325	arg1	voltammetry					332:342	square wave voltammetry	320:342	square wave voltammetry (SWV)	320:348	Electrochemical chiral interface based on two polysaccharides, soluble starch (SS) and chitosan (CS), was fabricated and used for chiral recognition of tyrosine (Tyr) enantiomers via square wave voltammetry (SWV).
30625594	3	14	from	SS-CS/GCE	676:684	arg1	1.38					654:657	1.38	654:657	1.38	654:657	Under the optimized experimental conditions, the oxidation peak current ratio of L-Tyr to D-Tyr (IL/ID) and the difference between the peak potential (ΔEp = ED - EL) were observed to be 1.38 and 12 mV at the SS-CS/GCE.
30625594	3	14	from	SS-CS/GCE	676:684	arg1	difference					580:589	the difference	576:589	the difference between the peak potential (ΔEp = ED - EL)	576:632	Under the optimized experimental conditions, the oxidation peak current ratio of L-Tyr to D-Tyr (IL/ID) and the difference between the peak potential (ΔEp = ED - EL) were observed to be 1.38 and 12 mV at the SS-CS/GCE.
30625594	3	14	from	SS-CS/GCE	676:684	arg1	ratio					540:544	the oxidation peak current ratio	513:544	the oxidation peak current ratio of L-Tyr to D-Tyr (IL/ID)	513:570	Under the optimized experimental conditions, the oxidation peak current ratio of L-Tyr to D-Tyr (IL/ID) and the difference between the peak potential (ΔEp = ED - EL) were observed to be 1.38 and 12 mV at the SS-CS/GCE.
30625594	7	15	theme	compounds	1334:1342	arg1	concentrations					1273:1286	10-fold higher concentrations	1258:1286	10-fold higher concentrations of physiological substances and various metal compounds	1258:1342	The proposed SS-CS/GCE-based chiral sensor exhibited high anti-interference ability in the presence of 10-fold higher concentrations of physiological substances and various metal compounds.
30625594	1	16	theme	wave	327:330	arg1	SWV					345:347	SWV	345:347	SWV	345:347	Electrochemical chiral interface based on two polysaccharides, soluble starch (SS) and chitosan (CS), was fabricated and used for chiral recognition of tyrosine (Tyr) enantiomers via square wave voltammetry (SWV).
30625594	1	16	theme	wave	327:330	arg1	voltammetry					332:342	square wave voltammetry	320:342	square wave voltammetry (SWV)	320:348	Electrochemical chiral interface based on two polysaccharides, soluble starch (SS) and chitosan (CS), was fabricated and used for chiral recognition of tyrosine (Tyr) enantiomers via square wave voltammetry (SWV).
30625594	4	17	theme	Tyr	735:737	arg1	concentration					750:762	Tyr enantiomer concentration	735:762	Tyr enantiomer concentration	735:762	In addition, a good linear relationship between Tyr enantiomer concentration and peak current could be observed.
30625594	7	18	theme	anti-interference	1213:1229	arg1	ability					1231:1237	high anti-interference ability	1208:1237	high anti-interference ability	1208:1237	The proposed SS-CS/GCE-based chiral sensor exhibited high anti-interference ability in the presence of 10-fold higher concentrations of physiological substances and various metal compounds.
30625594	3	19	theme	optimized	478:486	arg1	conditions					501:510	the optimized experimental conditions	474:510	the optimized experimental conditions	474:510	Under the optimized experimental conditions, the oxidation peak current ratio of L-Tyr to D-Tyr (IL/ID) and the difference between the peak potential (ΔEp = ED - EL) were observed to be 1.38 and 12 mV at the SS-CS/GCE.
30625594	7	20	dep	concentrations	1273:1286	arg1	the					1242:1244	the	1242:1244	the	1242:1244	The proposed SS-CS/GCE-based chiral sensor exhibited high anti-interference ability in the presence of 10-fold higher concentrations of physiological substances and various metal compounds.
30625594	7	20	dep	concentrations	1273:1286	arg1	presence					1246:1253	presence	1246:1253	presence	1246:1253	The proposed SS-CS/GCE-based chiral sensor exhibited high anti-interference ability in the presence of 10-fold higher concentrations of physiological substances and various metal compounds.
30625594	1	21	used	used	258:261	arg2	interface					160:168	Electrochemical chiral interface	137:168	Electrochemical chiral interface based on two polysaccharides, soluble starch (SS) and chitosan (CS),	137:237	Electrochemical chiral interface based on two polysaccharides, soluble starch (SS) and chitosan (CS), was fabricated and used for chiral recognition of tyrosine (Tyr) enantiomers via square wave voltammetry (SWV).
30625594	6	22	theme	enantiomers	1142:1152	arg1	determination					1121:1133	qualitative and quantitative determination	1092:1133	qualitative and quantitative determination of Tyr enantiomers	1092:1152	The novel SS-CS/GCE showed an ability to predict the ratios of L- and D-Tyr in a racemic mixture and exhibited the possibility of qualitative and quantitative determination of Tyr enantiomers.
30625594	3	23	theme	experimental	488:499	arg1	conditions					501:510	the optimized experimental conditions	474:510	the optimized experimental conditions	474:510	Under the optimized experimental conditions, the oxidation peak current ratio of L-Tyr to D-Tyr (IL/ID) and the difference between the peak potential (ΔEp = ED - EL) were observed to be 1.38 and 12 mV at the SS-CS/GCE.
30625594	8	24	theme	excellent	1403:1411	arg1	reproducibility					1413:1427	excellent reproducibility	1403:1427	excellent reproducibility	1403:1427	Meanwhile, the SS-CS/GCE possessed good repeatability and excellent reproducibility.
30625594	6	25	theme	novel	966:970	arg1	SS-CS/GCE					972:980	The novel SS-CS/GCE	962:980	The novel SS-CS/GCE	962:980	The novel SS-CS/GCE showed an ability to predict the ratios of L- and D-Tyr in a racemic mixture and exhibited the possibility of qualitative and quantitative determination of Tyr enantiomers.
30625594	0	26	theme	novel	2:6	arg1	interface					31:39	A novel electrochemical chiral interface	0:39	A novel electrochemical chiral interface	0:39	A novel electrochemical chiral interface based on the synergistic effect of polysaccharides for the recognition of tyrosine enantiomers.
30625594	6	27	theme	Tyr	1138:1140	arg1	enantiomers					1142:1152	Tyr enantiomers	1138:1152	Tyr enantiomers	1138:1152	The novel SS-CS/GCE showed an ability to predict the ratios of L- and D-Tyr in a racemic mixture and exhibited the possibility of qualitative and quantitative determination of Tyr enantiomers.
30625594	3	28	theme	peak	527:530	arg1	1.38					654:657	1.38	654:657	1.38	654:657	Under the optimized experimental conditions, the oxidation peak current ratio of L-Tyr to D-Tyr (IL/ID) and the difference between the peak potential (ΔEp = ED - EL) were observed to be 1.38 and 12 mV at the SS-CS/GCE.
30625594	3	28	theme	peak	527:530	arg1	ratio					540:544	the oxidation peak current ratio	513:544	the oxidation peak current ratio of L-Tyr to D-Tyr (IL/ID)	513:570	Under the optimized experimental conditions, the oxidation peak current ratio of L-Tyr to D-Tyr (IL/ID) and the difference between the peak potential (ΔEp = ED - EL) were observed to be 1.38 and 12 mV at the SS-CS/GCE.
30625594	7	29	theme	proposed	1159:1166	arg1	sensor					1191:1196	The proposed SS-CS/GCE-based chiral sensor	1155:1196	The proposed SS-CS/GCE-based chiral sensor	1155:1196	The proposed SS-CS/GCE-based chiral sensor exhibited high anti-interference ability in the presence of 10-fold higher concentrations of physiological substances and various metal compounds.
30625594	2	30	theme	electron	401:408	arg1	SEM					422:424	SEM	422:424	SEM	422:424	The SS-CS composite was characterized by scanning electron microscopy (SEM) and Brunauer-Emmett-Teller (BET) method.
30625594	2	30	theme	electron	401:408	arg1	microscopy					410:419	scanning electron microscopy	392:419	scanning electron microscopy (SEM)	392:425	The SS-CS composite was characterized by scanning electron microscopy (SEM) and Brunauer-Emmett-Teller (BET) method.
30625594	4	31	theme	enantiomer	739:748	arg1	concentration					750:762	Tyr enantiomer concentration	735:762	Tyr enantiomer concentration	735:762	In addition, a good linear relationship between Tyr enantiomer concentration and peak current could be observed.
30625594	3	32	theme	current	532:538	arg1	1.38					654:657	1.38	654:657	1.38	654:657	Under the optimized experimental conditions, the oxidation peak current ratio of L-Tyr to D-Tyr (IL/ID) and the difference between the peak potential (ΔEp = ED - EL) were observed to be 1.38 and 12 mV at the SS-CS/GCE.
30625594	3	32	theme	current	532:538	arg1	ratio					540:544	the oxidation peak current ratio	513:544	the oxidation peak current ratio of L-Tyr to D-Tyr (IL/ID)	513:570	Under the optimized experimental conditions, the oxidation peak current ratio of L-Tyr to D-Tyr (IL/ID) and the difference between the peak potential (ΔEp = ED - EL) were observed to be 1.38 and 12 mV at the SS-CS/GCE.
30625594	1	33	theme	soluble	200:206	arg1	SS					216:217	SS	216:217	SS	216:217	Electrochemical chiral interface based on two polysaccharides, soluble starch (SS) and chitosan (CS), was fabricated and used for chiral recognition of tyrosine (Tyr) enantiomers via square wave voltammetry (SWV).
30625594	1	33	theme	soluble	200:206	arg1	polysaccharides					183:197	two polysaccharides	179:197	two polysaccharides	179:197	Electrochemical chiral interface based on two polysaccharides, soluble starch (SS) and chitosan (CS), was fabricated and used for chiral recognition of tyrosine (Tyr) enantiomers via square wave voltammetry (SWV).
30625594	1	33	theme	soluble	200:206	arg1	starch					208:213	soluble starch	200:213	soluble starch (SS)	200:218	Electrochemical chiral interface based on two polysaccharides, soluble starch (SS) and chitosan (CS), was fabricated and used for chiral recognition of tyrosine (Tyr) enantiomers via square wave voltammetry (SWV).
30625594	2	34	theme	scanning	392:399	arg1	SEM					422:424	SEM	422:424	SEM	422:424	The SS-CS composite was characterized by scanning electron microscopy (SEM) and Brunauer-Emmett-Teller (BET) method.
30625594	2	34	theme	scanning	392:399	arg1	microscopy					410:419	scanning electron microscopy	392:419	scanning electron microscopy (SEM)	392:425	The SS-CS composite was characterized by scanning electron microscopy (SEM) and Brunauer-Emmett-Teller (BET) method.
30625594	0	35	theme	enantiomers	124:134	arg1	recognition					100:110	the recognition	96:110	the recognition of tyrosine enantiomers	96:134	A novel electrochemical chiral interface based on the synergistic effect of polysaccharides for the recognition of tyrosine enantiomers.
30625594	6	36	theme	L-	1025:1026	arg1	ratios					1015:1020	the ratios	1011:1020	the ratios of L- and D-Tyr in a racemic mixture	1011:1057	The novel SS-CS/GCE showed an ability to predict the ratios of L- and D-Tyr in a racemic mixture and exhibited the possibility of qualitative and quantitative determination of Tyr enantiomers.
30625594	7	37	theme	various	1320:1326	arg1	compounds					1334:1342	various metal compounds	1320:1342	various metal compounds	1320:1342	The proposed SS-CS/GCE-based chiral sensor exhibited high anti-interference ability in the presence of 10-fold higher concentrations of physiological substances and various metal compounds.
30625594	4	38	located	observed	790:797	arg1	addition					690:697	addition	690:697	addition	690:697	In addition, a good linear relationship between Tyr enantiomer concentration and peak current could be observed.
30625594	4	38	located	observed	790:797	arg2	relationship					714:725	a good linear relationship	700:725	a good linear relationship between Tyr enantiomer concentration and peak current	700:779	In addition, a good linear relationship between Tyr enantiomer concentration and peak current could be observed.
30625594	7	39	theme	high	1208:1211	arg1	ability					1231:1237	high anti-interference ability	1208:1237	high anti-interference ability	1208:1237	The proposed SS-CS/GCE-based chiral sensor exhibited high anti-interference ability in the presence of 10-fold higher concentrations of physiological substances and various metal compounds.
30625594	0	40	theme	electrochemical	8:22	arg1	interface					31:39	A novel electrochemical chiral interface	0:39	A novel electrochemical chiral interface	0:39	A novel electrochemical chiral interface based on the synergistic effect of polysaccharides for the recognition of tyrosine enantiomers.
30625594	4	41	theme	good	702:705	arg1	relationship					714:725	a good linear relationship	700:725	a good linear relationship between Tyr enantiomer concentration and peak current	700:779	In addition, a good linear relationship between Tyr enantiomer concentration and peak current could be observed.
30625594	0	42	theme	tyrosine	115:122	arg1	enantiomers					124:134	tyrosine enantiomers	115:134	tyrosine enantiomers	115:134	A novel electrochemical chiral interface based on the synergistic effect of polysaccharides for the recognition of tyrosine enantiomers.
30625594	8	43	theme	good	1380:1383	arg1	repeatability					1385:1397	good repeatability	1380:1397	good repeatability	1380:1397	Meanwhile, the SS-CS/GCE possessed good repeatability and excellent reproducibility.
30625594	3	44	theme	oxidation	517:525	arg1	1.38					654:657	1.38	654:657	1.38	654:657	Under the optimized experimental conditions, the oxidation peak current ratio of L-Tyr to D-Tyr (IL/ID) and the difference between the peak potential (ΔEp = ED - EL) were observed to be 1.38 and 12 mV at the SS-CS/GCE.
30625594	3	44	theme	oxidation	517:525	arg1	ratio					540:544	the oxidation peak current ratio	513:544	the oxidation peak current ratio of L-Tyr to D-Tyr (IL/ID)	513:570	Under the optimized experimental conditions, the oxidation peak current ratio of L-Tyr to D-Tyr (IL/ID) and the difference between the peak potential (ΔEp = ED - EL) were observed to be 1.38 and 12 mV at the SS-CS/GCE.
30625594	6	45	theme	D-Tyr	1032:1036	arg1	ratios					1015:1020	the ratios	1011:1020	the ratios of L- and D-Tyr in a racemic mixture	1011:1057	The novel SS-CS/GCE showed an ability to predict the ratios of L- and D-Tyr in a racemic mixture and exhibited the possibility of qualitative and quantitative determination of Tyr enantiomers.
30625594	1	46	theme	chiral	267:272	arg1	recognition					274:284	chiral recognition	267:284	chiral recognition of tyrosine (Tyr) enantiomers	267:314	Electrochemical chiral interface based on two polysaccharides, soluble starch (SS) and chitosan (CS), was fabricated and used for chiral recognition of tyrosine (Tyr) enantiomers via square wave voltammetry (SWV).
30625594	3	47	theme	L-Tyr	549:553	arg1	difference					580:589	the difference	576:589	the difference between the peak potential (ΔEp = ED - EL)	576:632	Under the optimized experimental conditions, the oxidation peak current ratio of L-Tyr to D-Tyr (IL/ID) and the difference between the peak potential (ΔEp = ED - EL) were observed to be 1.38 and 12 mV at the SS-CS/GCE.
30625594	3	47	theme	L-Tyr	549:553	arg1	1.38					654:657	1.38	654:657	1.38	654:657	Under the optimized experimental conditions, the oxidation peak current ratio of L-Tyr to D-Tyr (IL/ID) and the difference between the peak potential (ΔEp = ED - EL) were observed to be 1.38 and 12 mV at the SS-CS/GCE.
30625594	3	47	theme	L-Tyr	549:553	arg1	ratio					540:544	the oxidation peak current ratio	513:544	the oxidation peak current ratio of L-Tyr to D-Tyr (IL/ID)	513:570	Under the optimized experimental conditions, the oxidation peak current ratio of L-Tyr to D-Tyr (IL/ID) and the difference between the peak potential (ΔEp = ED - EL) were observed to be 1.38 and 12 mV at the SS-CS/GCE.
30625594	0	48	theme	chiral	24:29	arg1	interface					31:39	A novel electrochemical chiral interface	0:39	A novel electrochemical chiral interface	0:39	A novel electrochemical chiral interface based on the synergistic effect of polysaccharides for the recognition of tyrosine enantiomers.
30625594	7	49	theme	physiological	1291:1303	arg1	substances					1305:1314	physiological substances	1291:1314	physiological substances	1291:1314	The proposed SS-CS/GCE-based chiral sensor exhibited high anti-interference ability in the presence of 10-fold higher concentrations of physiological substances and various metal compounds.
30625594	2	50	theme	SS-CS	355:359	arg1	composite					361:369	The SS-CS composite	351:369	The SS-CS composite	351:369	The SS-CS composite was characterized by scanning electron microscopy (SEM) and Brunauer-Emmett-Teller (BET) method.
30625594	0	51	theme	synergistic	54:64	arg1	effect					66:71	the synergistic effect	50:71	the synergistic effect of polysaccharides for the recognition of tyrosine enantiomers	50:134	A novel electrochemical chiral interface based on the synergistic effect of polysaccharides for the recognition of tyrosine enantiomers.
30625594	7	52	theme	SS-CS/GCE-based	1168:1182	arg1	sensor					1191:1196	The proposed SS-CS/GCE-based chiral sensor	1155:1196	The proposed SS-CS/GCE-based chiral sensor	1155:1196	The proposed SS-CS/GCE-based chiral sensor exhibited high anti-interference ability in the presence of 10-fold higher concentrations of physiological substances and various metal compounds.
30625594	1	53	theme	tyrosine	289:296	arg1	enantiomers					304:314	tyrosine (Tyr) enantiomers	289:314	tyrosine (Tyr) enantiomers	289:314	Electrochemical chiral interface based on two polysaccharides, soluble starch (SS) and chitosan (CS), was fabricated and used for chiral recognition of tyrosine (Tyr) enantiomers via square wave voltammetry (SWV).
30625594	3	54	theme	ED	625:626	arg1	potential					608:616	the peak potential	599:616	the peak potential (ΔEp = ED - EL)	599:632	Under the optimized experimental conditions, the oxidation peak current ratio of L-Tyr to D-Tyr (IL/ID) and the difference between the peak potential (ΔEp = ED - EL) were observed to be 1.38 and 12 mV at the SS-CS/GCE.
30625594	3	54	theme	ED	625:626	arg1	EL					630:631	ΔEp = ED - EL	619:631	ΔEp = ED - EL	619:631	Under the optimized experimental conditions, the oxidation peak current ratio of L-Tyr to D-Tyr (IL/ID) and the difference between the peak potential (ΔEp = ED - EL) were observed to be 1.38 and 12 mV at the SS-CS/GCE.
30625594	4	55	theme	linear	707:712	arg1	relationship					714:725	a good linear relationship	700:725	a good linear relationship between Tyr enantiomer concentration and peak current	700:779	In addition, a good linear relationship between Tyr enantiomer concentration and peak current could be observed.
30625594	7	56	theme	chiral	1184:1189	arg1	sensor					1191:1196	The proposed SS-CS/GCE-based chiral sensor	1155:1196	The proposed SS-CS/GCE-based chiral sensor	1155:1196	The proposed SS-CS/GCE-based chiral sensor exhibited high anti-interference ability in the presence of 10-fold higher concentrations of physiological substances and various metal compounds.
30625594	6	57	theme	qualitative	1092:1102	arg1	determination					1121:1133	qualitative and quantitative determination	1092:1133	qualitative and quantitative determination of Tyr enantiomers	1092:1152	The novel SS-CS/GCE showed an ability to predict the ratios of L- and D-Tyr in a racemic mixture and exhibited the possibility of qualitative and quantitative determination of Tyr enantiomers.
30625594	3	58	theme	ΔEp	619:621	arg1	potential					608:616	the peak potential	599:616	the peak potential (ΔEp = ED - EL)	599:632	Under the optimized experimental conditions, the oxidation peak current ratio of L-Tyr to D-Tyr (IL/ID) and the difference between the peak potential (ΔEp = ED - EL) were observed to be 1.38 and 12 mV at the SS-CS/GCE.
30625594	3	58	theme	ΔEp	619:621	arg1	EL					630:631	ΔEp = ED - EL	619:631	ΔEp = ED - EL	619:631	Under the optimized experimental conditions, the oxidation peak current ratio of L-Tyr to D-Tyr (IL/ID) and the difference between the peak potential (ΔEp = ED - EL) were observed to be 1.38 and 12 mV at the SS-CS/GCE.
30625594	5	59	theme	concentration	928:940	arg1	range					942:946	the concentration range 0.01-1.00 mM	924:959	the concentration range 0.01-1.00 mM	924:959	The SS-CS/GCE exhibited good coefficients of determination (R2D-Tyr = 0.99631 and R2L-Tyr = 0.98333) for D-Tyr and L-Tyr in the concentration range 0.01-1.00 mM.
30625594	5	60	theme	=	890:890	arg1	0.98333					892:898	R2D-Tyr = 0.99631 and R2L-Tyr = 0.98333	860:898	0.98333	892:898	The SS-CS/GCE exhibited good coefficients of determination (R2D-Tyr = 0.99631 and R2L-Tyr = 0.98333) for D-Tyr and L-Tyr in the concentration range 0.01-1.00 mM.
30625594	6	61	theme	quantitative	1108:1119	arg1	determination					1121:1133	qualitative and quantitative determination	1092:1133	qualitative and quantitative determination of Tyr enantiomers	1092:1152	The novel SS-CS/GCE showed an ability to predict the ratios of L- and D-Tyr in a racemic mixture and exhibited the possibility of qualitative and quantitative determination of Tyr enantiomers.
30625594	5	62	theme	good	824:827	arg1	coefficients					829:840	good coefficients	824:840	good coefficients of determination (R2D-Tyr = 0.99631 and R2L-Tyr = 0.98333) for D-Tyr and L-Tyr	824:919	The SS-CS/GCE exhibited good coefficients of determination (R2D-Tyr = 0.99631 and R2L-Tyr = 0.98333) for D-Tyr and L-Tyr in the concentration range 0.01-1.00 mM.
30625594	7	63	theme	metal	1328:1332	arg1	compounds					1334:1342	various metal compounds	1320:1342	various metal compounds	1320:1342	The proposed SS-CS/GCE-based chiral sensor exhibited high anti-interference ability in the presence of 10-fold higher concentrations of physiological substances and various metal compounds.
30625594	5	64	theme	R2L-Tyr	882:888	arg1	0.98333					892:898	R2D-Tyr = 0.99631 and R2L-Tyr = 0.98333	860:898	0.98333	892:898	The SS-CS/GCE exhibited good coefficients of determination (R2D-Tyr = 0.99631 and R2L-Tyr = 0.98333) for D-Tyr and L-Tyr in the concentration range 0.01-1.00 mM.
30625594	7	65	theme	higher	1266:1271	arg1	concentrations					1273:1286	10-fold higher concentrations	1258:1286	10-fold higher concentrations of physiological substances and various metal compounds	1258:1342	The proposed SS-CS/GCE-based chiral sensor exhibited high anti-interference ability in the presence of 10-fold higher concentrations of physiological substances and various metal compounds.
30625594	1	66	theme	Electrochemical	137:151	arg1	interface					160:168	Electrochemical chiral interface	137:168	Electrochemical chiral interface based on two polysaccharides, soluble starch (SS) and chitosan (CS),	137:237	Electrochemical chiral interface based on two polysaccharides, soluble starch (SS) and chitosan (CS), was fabricated and used for chiral recognition of tyrosine (Tyr) enantiomers via square wave voltammetry (SWV).
30625594	3	67	theme	peak	603:606	arg1	potential					608:616	the peak potential	599:616	the peak potential (ΔEp = ED - EL)	599:632	Under the optimized experimental conditions, the oxidation peak current ratio of L-Tyr to D-Tyr (IL/ID) and the difference between the peak potential (ΔEp = ED - EL) were observed to be 1.38 and 12 mV at the SS-CS/GCE.
30625594	3	67	theme	peak	603:606	arg1	EL					630:631	ΔEp = ED - EL	619:631	ΔEp = ED - EL	619:631	Under the optimized experimental conditions, the oxidation peak current ratio of L-Tyr to D-Tyr (IL/ID) and the difference between the peak potential (ΔEp = ED - EL) were observed to be 1.38 and 12 mV at the SS-CS/GCE.
30625594	1	68	theme	chiral	153:158	arg1	interface					160:168	Electrochemical chiral interface	137:168	Electrochemical chiral interface based on two polysaccharides, soluble starch (SS) and chitosan (CS),	137:237	Electrochemical chiral interface based on two polysaccharides, soluble starch (SS) and chitosan (CS), was fabricated and used for chiral recognition of tyrosine (Tyr) enantiomers via square wave voltammetry (SWV).
30625594	6	69	theme	racemic	1043:1049	arg1	mixture					1051:1057	a racemic mixture	1041:1057	a racemic mixture	1041:1057	The novel SS-CS/GCE showed an ability to predict the ratios of L- and D-Tyr in a racemic mixture and exhibited the possibility of qualitative and quantitative determination of Tyr enantiomers.
30625594	2	70	theme	BET	455:457	arg1	method					460:465	Brunauer-Emmett-Teller (BET) method	431:465	Brunauer-Emmett-Teller (BET) method	431:465	The SS-CS composite was characterized by scanning electron microscopy (SEM) and Brunauer-Emmett-Teller (BET) method.
30625594	1	71	theme	Tyr	299:301	arg1	enantiomers					304:314	tyrosine (Tyr) enantiomers	289:314	tyrosine (Tyr) enantiomers	289:314	Electrochemical chiral interface based on two polysaccharides, soluble starch (SS) and chitosan (CS), was fabricated and used for chiral recognition of tyrosine (Tyr) enantiomers via square wave voltammetry (SWV).
35519496	2	0	theme	extraction	559:568	arg1	temperature					570:580	extraction temperature	559:580	extraction temperature at 47 °C	559:589	At a water-to-material fixed ratio of 30 mL g-1, the highest yield of 3.72 ± 0.13% was obtained under the optimum conditions of extraction time of 32 min, extraction temperature at 47 °C, and extraction pressure at 1.87 Mpa, which were in agreement with the predicted value of 3.683%.
35519496	4	1	theme	chemical	967:974	arg1	analysis					988:995	chemical composition analysis	967:995	chemical composition analysis	967:995	The preliminary characteristics of TQCPS were analyzed through UV-vis, FT-IR, and chemical composition analysis.
35519496	5	2	theme	oxide	1354:1358	arg1	synthase					1360:1367	inducible nitric oxide synthase	1337:1367	inducible nitric oxide synthase (iNOS)	1337:1374	In subsequent anti-inflammatory studies, when RAW 264.7 mouse macrophage cells were treated with TQCPS, satisfactory anti-inflammatory activity was observed, and TQCPS significantly suppressed the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) and synchronously restrained the expression levels of inducible nitric oxide synthase (iNOS), TNF-α, and IL-6 mRNA induced by lipopolysaccharide (LPS) in a dose-dependent manner.
35519496	5	2	theme	oxide	1354:1358	arg1	iNOS					1370:1373	iNOS	1370:1373	iNOS	1370:1373	In subsequent anti-inflammatory studies, when RAW 264.7 mouse macrophage cells were treated with TQCPS, satisfactory anti-inflammatory activity was observed, and TQCPS significantly suppressed the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) and synchronously restrained the expression levels of inducible nitric oxide synthase (iNOS), TNF-α, and IL-6 mRNA induced by lipopolysaccharide (LPS) in a dose-dependent manner.
35519496	1	3	theme	quadrispinosa	265:277	arg1	TQCPS					296:300	TQCPS	296:300	TQCPS	296:300	A novel method was developed using pressurized-assisted extraction (PAE) to efficiently extract crude Trapa quadrispinosa polysaccharides (TQCPS) from plant stems, and the extraction process was optimized using response surface methodology (RSM).
35519496	1	3	theme	quadrispinosa	265:277	arg1	polysaccharides					279:293	crude Trapa quadrispinosa polysaccharides	253:293	crude Trapa quadrispinosa polysaccharides (TQCPS)	253:301	A novel method was developed using pressurized-assisted extraction (PAE) to efficiently extract crude Trapa quadrispinosa polysaccharides (TQCPS) from plant stems, and the extraction process was optimized using response surface methodology (RSM).
35519496	2	4	theme	min	554:556	arg1	pressure					607:614	extraction pressure	596:614	extraction pressure at 1.87 Mpa, which were in agreement with the predicted value of 3.683%	596:686	At a water-to-material fixed ratio of 30 mL g-1, the highest yield of 3.72 ± 0.13% was obtained under the optimum conditions of extraction time of 32 min, extraction temperature at 47 °C, and extraction pressure at 1.87 Mpa, which were in agreement with the predicted value of 3.683%.
35519496	2	4	theme	min	554:556	arg1	time					543:546	extraction time	532:546	extraction time of 32 min	532:556	At a water-to-material fixed ratio of 30 mL g-1, the highest yield of 3.72 ± 0.13% was obtained under the optimum conditions of extraction time of 32 min, extraction temperature at 47 °C, and extraction pressure at 1.87 Mpa, which were in agreement with the predicted value of 3.683%.
35519496	2	4	theme	min	554:556	arg1	temperature					570:580	extraction temperature	559:580	extraction temperature at 47 °C	559:589	At a water-to-material fixed ratio of 30 mL g-1, the highest yield of 3.72 ± 0.13% was obtained under the optimum conditions of extraction time of 32 min, extraction temperature at 47 °C, and extraction pressure at 1.87 Mpa, which were in agreement with the predicted value of 3.683%.
35519496	2	5	theme	%	686:686	arg1	value					672:676	the predicted value	658:676	the predicted value of 3.683%	658:686	At a water-to-material fixed ratio of 30 mL g-1, the highest yield of 3.72 ± 0.13% was obtained under the optimum conditions of extraction time of 32 min, extraction temperature at 47 °C, and extraction pressure at 1.87 Mpa, which were in agreement with the predicted value of 3.683%.
35519496	6	6	from	agent	1653:1657	arg1	medicine					1662:1669	medicine	1662:1669	medicine	1662:1669	These results indicate that PAE is a technology that can be used for efficient extraction of polysaccharides from medicinal plants, and TQCPS can be explored as a potential anti-inflammatory agent in medicine.
35519496	5	7	theme	inducible	1337:1345	arg1	synthase					1360:1367	inducible nitric oxide synthase	1337:1367	inducible nitric oxide synthase (iNOS)	1337:1374	In subsequent anti-inflammatory studies, when RAW 264.7 mouse macrophage cells were treated with TQCPS, satisfactory anti-inflammatory activity was observed, and TQCPS significantly suppressed the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) and synchronously restrained the expression levels of inducible nitric oxide synthase (iNOS), TNF-α, and IL-6 mRNA induced by lipopolysaccharide (LPS) in a dose-dependent manner.
35519496	5	7	theme	inducible	1337:1345	arg1	iNOS					1370:1373	iNOS	1370:1373	iNOS	1370:1373	In subsequent anti-inflammatory studies, when RAW 264.7 mouse macrophage cells were treated with TQCPS, satisfactory anti-inflammatory activity was observed, and TQCPS significantly suppressed the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) and synchronously restrained the expression levels of inducible nitric oxide synthase (iNOS), TNF-α, and IL-6 mRNA induced by lipopolysaccharide (LPS) in a dose-dependent manner.
35519496	1	8	theme	pressurized-assisted	192:211	arg1	PAE					225:227	PAE	225:227	PAE	225:227	A novel method was developed using pressurized-assisted extraction (PAE) to efficiently extract crude Trapa quadrispinosa polysaccharides (TQCPS) from plant stems, and the extraction process was optimized using response surface methodology (RSM).
35519496	1	8	theme	pressurized-assisted	192:211	arg1	extraction					213:222	pressurized-assisted extraction	192:222	pressurized-assisted extraction (PAE)	192:228	A novel method was developed using pressurized-assisted extraction (PAE) to efficiently extract crude Trapa quadrispinosa polysaccharides (TQCPS) from plant stems, and the extraction process was optimized using response surface methodology (RSM).
35519496	0	9	from	activity	84:91	arg1	stems					127:131	the stems	123:131	the stems of Trapa quadrispinosa	123:154	Optimization of the extraction, preliminary characterization, and anti-inflammatory activity of crude polysaccharides from the stems of Trapa quadrispinosa.
35519496	3	10	theme	conventional	703:714	arg1	HWE					738:740	HWE	738:740	HWE	738:740	Compared with conventional hot water extraction (HWE), the PAE method remarkably enhanced the extraction yield with the further advantages of short extraction time and low extraction temperature.
35519496	3	10	theme	conventional	703:714	arg1	extraction					726:735	conventional hot water extraction	703:735	conventional hot water extraction (HWE)	703:741	Compared with conventional hot water extraction (HWE), the PAE method remarkably enhanced the extraction yield with the further advantages of short extraction time and low extraction temperature.
35519496	0	11	theme	Trapa	136:140	arg1	quadrispinosa					142:154	Trapa quadrispinosa	136:154	Trapa quadrispinosa	136:154	Optimization of the extraction, preliminary characterization, and anti-inflammatory activity of crude polysaccharides from the stems of Trapa quadrispinosa.
35519496	5	12	theme	anti-inflammatory	1012:1028	arg1	studies					1030:1036	subsequent anti-inflammatory studies	1001:1036	subsequent anti-inflammatory studies	1001:1036	In subsequent anti-inflammatory studies, when RAW 264.7 mouse macrophage cells were treated with TQCPS, satisfactory anti-inflammatory activity was observed, and TQCPS significantly suppressed the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) and synchronously restrained the expression levels of inducible nitric oxide synthase (iNOS), TNF-α, and IL-6 mRNA induced by lipopolysaccharide (LPS) in a dose-dependent manner.
35519496	2	13	with	agreement	643:651	arg1	value					672:676	the predicted value	658:676	the predicted value of 3.683%	658:686	At a water-to-material fixed ratio of 30 mL g-1, the highest yield of 3.72 ± 0.13% was obtained under the optimum conditions of extraction time of 32 min, extraction temperature at 47 °C, and extraction pressure at 1.87 Mpa, which were in agreement with the predicted value of 3.683%.
35519496	6	14	theme	medicinal	1576:1584	arg1	plants					1586:1591	medicinal plants	1576:1591	medicinal plants	1576:1591	These results indicate that PAE is a technology that can be used for efficient extraction of polysaccharides from medicinal plants, and TQCPS can be explored as a potential anti-inflammatory agent in medicine.
35519496	6	15	theme	anti-inflammatory	1635:1651	arg1	agent					1653:1657	a potential anti-inflammatory agent	1623:1657	a potential anti-inflammatory agent in medicine	1623:1669	These results indicate that PAE is a technology that can be used for efficient extraction of polysaccharides from medicinal plants, and TQCPS can be explored as a potential anti-inflammatory agent in medicine.
35519496	6	15	theme	anti-inflammatory	1635:1651	arg1	TQCPS					1598:1602	TQCPS	1598:1602	TQCPS	1598:1602	These results indicate that PAE is a technology that can be used for efficient extraction of polysaccharides from medicinal plants, and TQCPS can be explored as a potential anti-inflammatory agent in medicine.
35519496	2	16	theme	extraction	532:541	arg1	time					543:546	extraction time	532:546	extraction time of 32 min	532:556	At a water-to-material fixed ratio of 30 mL g-1, the highest yield of 3.72 ± 0.13% was obtained under the optimum conditions of extraction time of 32 min, extraction temperature at 47 °C, and extraction pressure at 1.87 Mpa, which were in agreement with the predicted value of 3.683%.
35519496	6	17	theme	polysaccharides	1555:1569	arg1	extraction					1541:1550	efficient extraction	1531:1550	efficient extraction of polysaccharides from medicinal plants	1531:1591	These results indicate that PAE is a technology that can be used for efficient extraction of polysaccharides from medicinal plants, and TQCPS can be explored as a potential anti-inflammatory agent in medicine.
35519496	2	18	theme	predicted	662:670	arg1	value					672:676	the predicted value	658:676	the predicted value of 3.683%	658:686	At a water-to-material fixed ratio of 30 mL g-1, the highest yield of 3.72 ± 0.13% was obtained under the optimum conditions of extraction time of 32 min, extraction temperature at 47 °C, and extraction pressure at 1.87 Mpa, which were in agreement with the predicted value of 3.683%.
35519496	5	19	theme	tumor	1225:1229	arg1	TNF-α					1250:1254	TNF-α	1250:1254	TNF-α	1250:1254	In subsequent anti-inflammatory studies, when RAW 264.7 mouse macrophage cells were treated with TQCPS, satisfactory anti-inflammatory activity was observed, and TQCPS significantly suppressed the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) and synchronously restrained the expression levels of inducible nitric oxide synthase (iNOS), TNF-α, and IL-6 mRNA induced by lipopolysaccharide (LPS) in a dose-dependent manner.
35519496	5	19	theme	tumor	1225:1229	arg1	factor-α					1240:1247	tumor necrosis factor-α	1225:1247	tumor necrosis factor-α (TNF-α)	1225:1255	In subsequent anti-inflammatory studies, when RAW 264.7 mouse macrophage cells were treated with TQCPS, satisfactory anti-inflammatory activity was observed, and TQCPS significantly suppressed the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) and synchronously restrained the expression levels of inducible nitric oxide synthase (iNOS), TNF-α, and IL-6 mRNA induced by lipopolysaccharide (LPS) in a dose-dependent manner.
35519496	3	20	theme	water	720:724	arg1	HWE					738:740	HWE	738:740	HWE	738:740	Compared with conventional hot water extraction (HWE), the PAE method remarkably enhanced the extraction yield with the further advantages of short extraction time and low extraction temperature.
35519496	3	20	theme	water	720:724	arg1	extraction					726:735	conventional hot water extraction	703:735	conventional hot water extraction (HWE)	703:741	Compared with conventional hot water extraction (HWE), the PAE method remarkably enhanced the extraction yield with the further advantages of short extraction time and low extraction temperature.
35519496	5	21	theme	mouse	1054:1058	arg1	cells					1071:1075	RAW 264.7 mouse macrophage cells	1044:1075	RAW 264.7 mouse macrophage cells	1044:1075	In subsequent anti-inflammatory studies, when RAW 264.7 mouse macrophage cells were treated with TQCPS, satisfactory anti-inflammatory activity was observed, and TQCPS significantly suppressed the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) and synchronously restrained the expression levels of inducible nitric oxide synthase (iNOS), TNF-α, and IL-6 mRNA induced by lipopolysaccharide (LPS) in a dose-dependent manner.
35519496	4	22	theme	TQCPS	920:924	arg1	characteristics					901:915	The preliminary characteristics	885:915	The preliminary characteristics of TQCPS	885:924	The preliminary characteristics of TQCPS were analyzed through UV-vis, FT-IR, and chemical composition analysis.
35519496	6	23	theme	potential	1625:1633	arg1	agent					1653:1657	a potential anti-inflammatory agent	1623:1657	a potential anti-inflammatory agent in medicine	1623:1669	These results indicate that PAE is a technology that can be used for efficient extraction of polysaccharides from medicinal plants, and TQCPS can be explored as a potential anti-inflammatory agent in medicine.
35519496	6	23	theme	potential	1625:1633	arg1	TQCPS					1598:1602	TQCPS	1598:1602	TQCPS	1598:1602	These results indicate that PAE is a technology that can be used for efficient extraction of polysaccharides from medicinal plants, and TQCPS can be explored as a potential anti-inflammatory agent in medicine.
35519496	5	24	theme	nitric	1206:1211	arg1	oxide					1213:1217	nitric oxide	1206:1217	nitric oxide (NO)	1206:1222	In subsequent anti-inflammatory studies, when RAW 264.7 mouse macrophage cells were treated with TQCPS, satisfactory anti-inflammatory activity was observed, and TQCPS significantly suppressed the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) and synchronously restrained the expression levels of inducible nitric oxide synthase (iNOS), TNF-α, and IL-6 mRNA induced by lipopolysaccharide (LPS) in a dose-dependent manner.
35519496	5	24	theme	nitric	1206:1211	arg1	NO					1220:1221	NO	1220:1221	NO	1220:1221	In subsequent anti-inflammatory studies, when RAW 264.7 mouse macrophage cells were treated with TQCPS, satisfactory anti-inflammatory activity was observed, and TQCPS significantly suppressed the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) and synchronously restrained the expression levels of inducible nitric oxide synthase (iNOS), TNF-α, and IL-6 mRNA induced by lipopolysaccharide (LPS) in a dose-dependent manner.
35519496	0	25	theme	activity	84:91	arg1	Optimization					0:11	Optimization	0:11	Optimization of the extraction, preliminary characterization, and anti-inflammatory activity of crude polysaccharides from the stems of Trapa quadrispinosa.	0:155	Optimization of the extraction, preliminary characterization, and anti-inflammatory activity of crude polysaccharides from the stems of Trapa quadrispinosa.
35519496	6	26	theme	efficient	1531:1539	arg1	extraction					1541:1550	efficient extraction	1531:1550	efficient extraction of polysaccharides from medicinal plants	1531:1591	These results indicate that PAE is a technology that can be used for efficient extraction of polysaccharides from medicinal plants, and TQCPS can be explored as a potential anti-inflammatory agent in medicine.
35519496	3	27	theme	extraction	783:792	arg1	yield					794:798	the extraction yield	779:798	the extraction yield with the further advantages of short extraction time and low extraction temperature	779:882	Compared with conventional hot water extraction (HWE), the PAE method remarkably enhanced the extraction yield with the further advantages of short extraction time and low extraction temperature.
35519496	5	28	theme	mRNA	1393:1396	arg1	levels					1327:1332	the expression levels	1312:1332	the expression levels of inducible nitric oxide synthase (iNOS), TNF-α, and IL-6 mRNA induced by lipopolysaccharide (LPS) in a dose-dependent manner	1312:1459	In subsequent anti-inflammatory studies, when RAW 264.7 mouse macrophage cells were treated with TQCPS, satisfactory anti-inflammatory activity was observed, and TQCPS significantly suppressed the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) and synchronously restrained the expression levels of inducible nitric oxide synthase (iNOS), TNF-α, and IL-6 mRNA induced by lipopolysaccharide (LPS) in a dose-dependent manner.
35519496	2	29	theme	extraction	596:605	arg1	pressure					607:614	extraction pressure	596:614	extraction pressure at 1.87 Mpa, which were in agreement with the predicted value of 3.683%	596:686	At a water-to-material fixed ratio of 30 mL g-1, the highest yield of 3.72 ± 0.13% was obtained under the optimum conditions of extraction time of 32 min, extraction temperature at 47 °C, and extraction pressure at 1.87 Mpa, which were in agreement with the predicted value of 3.683%.
35519496	0	30	theme	crude	96:100	arg1	polysaccharides					102:116	crude polysaccharides	96:116	crude polysaccharides from the stems of Trapa quadrispinosa	96:154	Optimization of the extraction, preliminary characterization, and anti-inflammatory activity of crude polysaccharides from the stems of Trapa quadrispinosa.
35519496	2	31	theme	%	485:485	arg1	yield					465:469	the highest yield	453:469	the highest yield of 3.72 ± 0.13%	453:485	At a water-to-material fixed ratio of 30 mL g-1, the highest yield of 3.72 ± 0.13% was obtained under the optimum conditions of extraction time of 32 min, extraction temperature at 47 °C, and extraction pressure at 1.87 Mpa, which were in agreement with the predicted value of 3.683%.
35519496	0	32	from	characterization	44:59	arg1	stems					127:131	the stems	123:131	the stems of Trapa quadrispinosa	123:154	Optimization of the extraction, preliminary characterization, and anti-inflammatory activity of crude polysaccharides from the stems of Trapa quadrispinosa.
35519496	1	33	theme	surface	377:383	arg1	RSM					398:400	RSM	398:400	RSM	398:400	A novel method was developed using pressurized-assisted extraction (PAE) to efficiently extract crude Trapa quadrispinosa polysaccharides (TQCPS) from plant stems, and the extraction process was optimized using response surface methodology (RSM).
35519496	1	33	theme	surface	377:383	arg1	methodology					385:395	response surface methodology	368:395	response surface methodology (RSM)	368:401	A novel method was developed using pressurized-assisted extraction (PAE) to efficiently extract crude Trapa quadrispinosa polysaccharides (TQCPS) from plant stems, and the extraction process was optimized using response surface methodology (RSM).
35519496	3	34	theme	further	809:815	arg1	advantages					817:826	the further advantages	805:826	the further advantages of short extraction time and low extraction temperature	805:882	Compared with conventional hot water extraction (HWE), the PAE method remarkably enhanced the extraction yield with the further advantages of short extraction time and low extraction temperature.
35519496	2	35	theme	mL	445:446	arg1	g-1					448:450	30 mL g-1	442:450	30 mL g-1	442:450	At a water-to-material fixed ratio of 30 mL g-1, the highest yield of 3.72 ± 0.13% was obtained under the optimum conditions of extraction time of 32 min, extraction temperature at 47 °C, and extraction pressure at 1.87 Mpa, which were in agreement with the predicted value of 3.683%.
35519496	5	36	theme	TNF-α	1377:1381	arg1	levels					1327:1332	the expression levels	1312:1332	the expression levels of inducible nitric oxide synthase (iNOS), TNF-α, and IL-6 mRNA induced by lipopolysaccharide (LPS) in a dose-dependent manner	1312:1459	In subsequent anti-inflammatory studies, when RAW 264.7 mouse macrophage cells were treated with TQCPS, satisfactory anti-inflammatory activity was observed, and TQCPS significantly suppressed the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) and synchronously restrained the expression levels of inducible nitric oxide synthase (iNOS), TNF-α, and IL-6 mRNA induced by lipopolysaccharide (LPS) in a dose-dependent manner.
35519496	5	37	theme	interleukin-6	1262:1274	arg1	release					1195:1201	the release	1191:1201	the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6)	1191:1281	In subsequent anti-inflammatory studies, when RAW 264.7 mouse macrophage cells were treated with TQCPS, satisfactory anti-inflammatory activity was observed, and TQCPS significantly suppressed the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) and synchronously restrained the expression levels of inducible nitric oxide synthase (iNOS), TNF-α, and IL-6 mRNA induced by lipopolysaccharide (LPS) in a dose-dependent manner.
35519496	5	38	theme	satisfactory	1102:1113	arg1	activity					1133:1140	satisfactory anti-inflammatory activity	1102:1140	satisfactory anti-inflammatory activity	1102:1140	In subsequent anti-inflammatory studies, when RAW 264.7 mouse macrophage cells were treated with TQCPS, satisfactory anti-inflammatory activity was observed, and TQCPS significantly suppressed the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) and synchronously restrained the expression levels of inducible nitric oxide synthase (iNOS), TNF-α, and IL-6 mRNA induced by lipopolysaccharide (LPS) in a dose-dependent manner.
35519496	5	39	theme	factor-α	1240:1247	arg1	release					1195:1201	the release	1191:1201	the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6)	1191:1281	In subsequent anti-inflammatory studies, when RAW 264.7 mouse macrophage cells were treated with TQCPS, satisfactory anti-inflammatory activity was observed, and TQCPS significantly suppressed the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) and synchronously restrained the expression levels of inducible nitric oxide synthase (iNOS), TNF-α, and IL-6 mRNA induced by lipopolysaccharide (LPS) in a dose-dependent manner.
35519496	2	40	theme	fixed	427:431	arg1	ratio					433:437	a water-to-material fixed ratio	407:437	a water-to-material fixed ratio of 30 mL g-1	407:450	At a water-to-material fixed ratio of 30 mL g-1, the highest yield of 3.72 ± 0.13% was obtained under the optimum conditions of extraction time of 32 min, extraction temperature at 47 °C, and extraction pressure at 1.87 Mpa, which were in agreement with the predicted value of 3.683%.
35519496	3	41	theme	extraction	837:846	arg1	time					848:851	short extraction time	831:851	short extraction time	831:851	Compared with conventional hot water extraction (HWE), the PAE method remarkably enhanced the extraction yield with the further advantages of short extraction time and low extraction temperature.
35519496	6	42	used	used	1522:1525	arg2	PAE					1490:1492	PAE	1490:1492	PAE	1490:1492	These results indicate that PAE is a technology that can be used for efficient extraction of polysaccharides from medicinal plants, and TQCPS can be explored as a potential anti-inflammatory agent in medicine.
35519496	6	42	used	used	1522:1525	arg2	explored					1611:1618	explored	1611:1618	can be explored as a potential anti-inflammatory agent in medicine	1604:1669	These results indicate that PAE is a technology that can be used for efficient extraction of polysaccharides from medicinal plants, and TQCPS can be explored as a potential anti-inflammatory agent in medicine.
35519496	6	42	used	used	1522:1525	arg2	technology					1499:1508	a technology	1497:1508	a technology that can be used for efficient extraction of polysaccharides from medicinal plants	1497:1591	These results indicate that PAE is a technology that can be used for efficient extraction of polysaccharides from medicinal plants, and TQCPS can be explored as a potential anti-inflammatory agent in medicine.
35519496	2	43	theme	highest	457:463	arg1	yield					465:469	the highest yield	453:469	the highest yield of 3.72 ± 0.13%	453:485	At a water-to-material fixed ratio of 30 mL g-1, the highest yield of 3.72 ± 0.13% was obtained under the optimum conditions of extraction time of 32 min, extraction temperature at 47 °C, and extraction pressure at 1.87 Mpa, which were in agreement with the predicted value of 3.683%.
35519496	2	44	theme	3.72	474:477	arg1	±					479:479	±	479:479	±	479:479	At a water-to-material fixed ratio of 30 mL g-1, the highest yield of 3.72 ± 0.13% was obtained under the optimum conditions of extraction time of 32 min, extraction temperature at 47 °C, and extraction pressure at 1.87 Mpa, which were in agreement with the predicted value of 3.683%.
35519496	0	45	theme	characterization	44:59	arg1	Optimization					0:11	Optimization	0:11	Optimization of the extraction, preliminary characterization, and anti-inflammatory activity of crude polysaccharides from the stems of Trapa quadrispinosa.	0:155	Optimization of the extraction, preliminary characterization, and anti-inflammatory activity of crude polysaccharides from the stems of Trapa quadrispinosa.
35519496	1	46	theme	extraction	329:338	arg1	process					340:346	the extraction process	325:346	the extraction process	325:346	A novel method was developed using pressurized-assisted extraction (PAE) to efficiently extract crude Trapa quadrispinosa polysaccharides (TQCPS) from plant stems, and the extraction process was optimized using response surface methodology (RSM).
35519496	3	47	theme	extraction	861:870	arg1	temperature					872:882	low extraction temperature	857:882	low extraction temperature	857:882	Compared with conventional hot water extraction (HWE), the PAE method remarkably enhanced the extraction yield with the further advantages of short extraction time and low extraction temperature.
35519496	5	48	theme	expression	1316:1325	arg1	levels					1327:1332	the expression levels	1312:1332	the expression levels of inducible nitric oxide synthase (iNOS), TNF-α, and IL-6 mRNA induced by lipopolysaccharide (LPS) in a dose-dependent manner	1312:1459	In subsequent anti-inflammatory studies, when RAW 264.7 mouse macrophage cells were treated with TQCPS, satisfactory anti-inflammatory activity was observed, and TQCPS significantly suppressed the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) and synchronously restrained the expression levels of inducible nitric oxide synthase (iNOS), TNF-α, and IL-6 mRNA induced by lipopolysaccharide (LPS) in a dose-dependent manner.
35519496	5	49	located	observed	1146:1153	arg2	activity					1133:1140	satisfactory anti-inflammatory activity	1102:1140	satisfactory anti-inflammatory activity	1102:1140	In subsequent anti-inflammatory studies, when RAW 264.7 mouse macrophage cells were treated with TQCPS, satisfactory anti-inflammatory activity was observed, and TQCPS significantly suppressed the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) and synchronously restrained the expression levels of inducible nitric oxide synthase (iNOS), TNF-α, and IL-6 mRNA induced by lipopolysaccharide (LPS) in a dose-dependent manner.
35519496	5	49	located	observed	1146:1153	arg1	studies					1030:1036	subsequent anti-inflammatory studies	1001:1036	subsequent anti-inflammatory studies	1001:1036	In subsequent anti-inflammatory studies, when RAW 264.7 mouse macrophage cells were treated with TQCPS, satisfactory anti-inflammatory activity was observed, and TQCPS significantly suppressed the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) and synchronously restrained the expression levels of inducible nitric oxide synthase (iNOS), TNF-α, and IL-6 mRNA induced by lipopolysaccharide (LPS) in a dose-dependent manner.
35519496	4	50	theme	composition	976:986	arg1	analysis					988:995	chemical composition analysis	967:995	chemical composition analysis	967:995	The preliminary characteristics of TQCPS were analyzed through UV-vis, FT-IR, and chemical composition analysis.
35519496	0	51	from	stems	127:131	arg1	activity					84:91	anti-inflammatory activity	66:91	anti-inflammatory activity	66:91	Optimization of the extraction, preliminary characterization, and anti-inflammatory activity of crude polysaccharides from the stems of Trapa quadrispinosa.
35519496	0	51	from	stems	127:131	arg1	polysaccharides					102:116	crude polysaccharides	96:116	crude polysaccharides from the stems of Trapa quadrispinosa	96:154	Optimization of the extraction, preliminary characterization, and anti-inflammatory activity of crude polysaccharides from the stems of Trapa quadrispinosa.
35519496	0	51	from	stems	127:131	arg1	extraction					20:29	extraction	20:29	extraction	20:29	Optimization of the extraction, preliminary characterization, and anti-inflammatory activity of crude polysaccharides from the stems of Trapa quadrispinosa.
35519496	0	51	from	stems	127:131	arg1	characterization					44:59	preliminary characterization	32:59	preliminary characterization	32:59	Optimization of the extraction, preliminary characterization, and anti-inflammatory activity of crude polysaccharides from the stems of Trapa quadrispinosa.
35519496	1	52	theme	Trapa	259:263	arg1	TQCPS					296:300	TQCPS	296:300	TQCPS	296:300	A novel method was developed using pressurized-assisted extraction (PAE) to efficiently extract crude Trapa quadrispinosa polysaccharides (TQCPS) from plant stems, and the extraction process was optimized using response surface methodology (RSM).
35519496	1	52	theme	Trapa	259:263	arg1	polysaccharides					279:293	crude Trapa quadrispinosa polysaccharides	253:293	crude Trapa quadrispinosa polysaccharides (TQCPS)	253:301	A novel method was developed using pressurized-assisted extraction (PAE) to efficiently extract crude Trapa quadrispinosa polysaccharides (TQCPS) from plant stems, and the extraction process was optimized using response surface methodology (RSM).
35519496	5	53	theme	macrophage	1060:1069	arg1	cells					1071:1075	RAW 264.7 mouse macrophage cells	1044:1075	RAW 264.7 mouse macrophage cells	1044:1075	In subsequent anti-inflammatory studies, when RAW 264.7 mouse macrophage cells were treated with TQCPS, satisfactory anti-inflammatory activity was observed, and TQCPS significantly suppressed the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) and synchronously restrained the expression levels of inducible nitric oxide synthase (iNOS), TNF-α, and IL-6 mRNA induced by lipopolysaccharide (LPS) in a dose-dependent manner.
35519496	1	54	from	stems	314:318	arg1	extract					245:251	extract	245:251	extract	245:251	A novel method was developed using pressurized-assisted extraction (PAE) to efficiently extract crude Trapa quadrispinosa polysaccharides (TQCPS) from plant stems, and the extraction process was optimized using response surface methodology (RSM).
35519496	5	55	theme	synthase	1360:1367	arg1	levels					1327:1332	the expression levels	1312:1332	the expression levels of inducible nitric oxide synthase (iNOS), TNF-α, and IL-6 mRNA induced by lipopolysaccharide (LPS) in a dose-dependent manner	1312:1459	In subsequent anti-inflammatory studies, when RAW 264.7 mouse macrophage cells were treated with TQCPS, satisfactory anti-inflammatory activity was observed, and TQCPS significantly suppressed the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) and synchronously restrained the expression levels of inducible nitric oxide synthase (iNOS), TNF-α, and IL-6 mRNA induced by lipopolysaccharide (LPS) in a dose-dependent manner.
35519496	2	56	theme	temperature	570:580	arg1	conditions					518:527	the optimum conditions	506:527	the optimum conditions of extraction time of 32 min, extraction temperature at 47 °C, and extraction pressure at 1.87 Mpa, which were in agreement with the predicted value of 3.683%	506:686	At a water-to-material fixed ratio of 30 mL g-1, the highest yield of 3.72 ± 0.13% was obtained under the optimum conditions of extraction time of 32 min, extraction temperature at 47 °C, and extraction pressure at 1.87 Mpa, which were in agreement with the predicted value of 3.683%.
35519496	5	57	theme	nitric	1347:1352	arg1	synthase					1360:1367	inducible nitric oxide synthase	1337:1367	inducible nitric oxide synthase (iNOS)	1337:1374	In subsequent anti-inflammatory studies, when RAW 264.7 mouse macrophage cells were treated with TQCPS, satisfactory anti-inflammatory activity was observed, and TQCPS significantly suppressed the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) and synchronously restrained the expression levels of inducible nitric oxide synthase (iNOS), TNF-α, and IL-6 mRNA induced by lipopolysaccharide (LPS) in a dose-dependent manner.
35519496	5	57	theme	nitric	1347:1352	arg1	iNOS					1370:1373	iNOS	1370:1373	iNOS	1370:1373	In subsequent anti-inflammatory studies, when RAW 264.7 mouse macrophage cells were treated with TQCPS, satisfactory anti-inflammatory activity was observed, and TQCPS significantly suppressed the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) and synchronously restrained the expression levels of inducible nitric oxide synthase (iNOS), TNF-α, and IL-6 mRNA induced by lipopolysaccharide (LPS) in a dose-dependent manner.
35519496	2	58	theme	optimum	510:516	arg1	conditions					518:527	the optimum conditions	506:527	the optimum conditions of extraction time of 32 min, extraction temperature at 47 °C, and extraction pressure at 1.87 Mpa, which were in agreement with the predicted value of 3.683%	506:686	At a water-to-material fixed ratio of 30 mL g-1, the highest yield of 3.72 ± 0.13% was obtained under the optimum conditions of extraction time of 32 min, extraction temperature at 47 °C, and extraction pressure at 1.87 Mpa, which were in agreement with the predicted value of 3.683%.
35519496	0	59	theme	quadrispinosa	142:154	arg1	stems					127:131	the stems	123:131	the stems of Trapa quadrispinosa	123:154	Optimization of the extraction, preliminary characterization, and anti-inflammatory activity of crude polysaccharides from the stems of Trapa quadrispinosa.
35519496	5	60	theme	subsequent	1001:1010	arg1	studies					1030:1036	subsequent anti-inflammatory studies	1001:1036	subsequent anti-inflammatory studies	1001:1036	In subsequent anti-inflammatory studies, when RAW 264.7 mouse macrophage cells were treated with TQCPS, satisfactory anti-inflammatory activity was observed, and TQCPS significantly suppressed the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) and synchronously restrained the expression levels of inducible nitric oxide synthase (iNOS), TNF-α, and IL-6 mRNA induced by lipopolysaccharide (LPS) in a dose-dependent manner.
35519496	3	61	theme	hot	716:718	arg1	HWE					738:740	HWE	738:740	HWE	738:740	Compared with conventional hot water extraction (HWE), the PAE method remarkably enhanced the extraction yield with the further advantages of short extraction time and low extraction temperature.
35519496	3	61	theme	hot	716:718	arg1	extraction					726:735	conventional hot water extraction	703:735	conventional hot water extraction (HWE)	703:741	Compared with conventional hot water extraction (HWE), the PAE method remarkably enhanced the extraction yield with the further advantages of short extraction time and low extraction temperature.
35519496	2	62	theme	time	543:546	arg1	conditions					518:527	the optimum conditions	506:527	the optimum conditions of extraction time of 32 min, extraction temperature at 47 °C, and extraction pressure at 1.87 Mpa, which were in agreement with the predicted value of 3.683%	506:686	At a water-to-material fixed ratio of 30 mL g-1, the highest yield of 3.72 ± 0.13% was obtained under the optimum conditions of extraction time of 32 min, extraction temperature at 47 °C, and extraction pressure at 1.87 Mpa, which were in agreement with the predicted value of 3.683%.
35519496	0	63	theme	anti-inflammatory	66:82	arg1	activity					84:91	anti-inflammatory activity	66:91	anti-inflammatory activity	66:91	Optimization of the extraction, preliminary characterization, and anti-inflammatory activity of crude polysaccharides from the stems of Trapa quadrispinosa.
35519496	4	64	theme	preliminary	889:899	arg1	characteristics					901:915	The preliminary characteristics	885:915	The preliminary characteristics of TQCPS	885:924	The preliminary characteristics of TQCPS were analyzed through UV-vis, FT-IR, and chemical composition analysis.
35519496	0	65	from	extraction	20:29	arg1	stems					127:131	the stems	123:131	the stems of Trapa quadrispinosa	123:154	Optimization of the extraction, preliminary characterization, and anti-inflammatory activity of crude polysaccharides from the stems of Trapa quadrispinosa.
35519496	3	66	with	yield	794:798	arg1	advantages					817:826	the further advantages	805:826	the further advantages of short extraction time and low extraction temperature	805:882	Compared with conventional hot water extraction (HWE), the PAE method remarkably enhanced the extraction yield with the further advantages of short extraction time and low extraction temperature.
35519496	1	67	theme	novel	159:163	arg1	method					165:170	A novel method	157:170	A novel method	157:170	A novel method was developed using pressurized-assisted extraction (PAE) to efficiently extract crude Trapa quadrispinosa polysaccharides (TQCPS) from plant stems, and the extraction process was optimized using response surface methodology (RSM).
35519496	5	68	theme	IL-6	1388:1391	arg1	mRNA					1393:1396	IL-6 mRNA	1388:1396	IL-6 mRNA	1388:1396	In subsequent anti-inflammatory studies, when RAW 264.7 mouse macrophage cells were treated with TQCPS, satisfactory anti-inflammatory activity was observed, and TQCPS significantly suppressed the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) and synchronously restrained the expression levels of inducible nitric oxide synthase (iNOS), TNF-α, and IL-6 mRNA induced by lipopolysaccharide (LPS) in a dose-dependent manner.
35519496	2	69	theme	pressure	607:614	arg1	conditions					518:527	the optimum conditions	506:527	the optimum conditions of extraction time of 32 min, extraction temperature at 47 °C, and extraction pressure at 1.87 Mpa, which were in agreement with the predicted value of 3.683%	506:686	At a water-to-material fixed ratio of 30 mL g-1, the highest yield of 3.72 ± 0.13% was obtained under the optimum conditions of extraction time of 32 min, extraction temperature at 47 °C, and extraction pressure at 1.87 Mpa, which were in agreement with the predicted value of 3.683%.
35519496	2	70	from	Mpa	624:626	arg1	conditions					518:527	the optimum conditions	506:527	the optimum conditions of extraction time of 32 min, extraction temperature at 47 °C, and extraction pressure at 1.87 Mpa, which were in agreement with the predicted value of 3.683%	506:686	At a water-to-material fixed ratio of 30 mL g-1, the highest yield of 3.72 ± 0.13% was obtained under the optimum conditions of extraction time of 32 min, extraction temperature at 47 °C, and extraction pressure at 1.87 Mpa, which were in agreement with the predicted value of 3.683%.
35519496	2	70	from	Mpa	624:626	arg1	time					543:546	extraction time	532:546	extraction time of 32 min	532:556	At a water-to-material fixed ratio of 30 mL g-1, the highest yield of 3.72 ± 0.13% was obtained under the optimum conditions of extraction time of 32 min, extraction temperature at 47 °C, and extraction pressure at 1.87 Mpa, which were in agreement with the predicted value of 3.683%.
35519496	2	70	from	Mpa	624:626	arg1	pressure					607:614	extraction pressure	596:614	extraction pressure at 1.87 Mpa, which were in agreement with the predicted value of 3.683%	596:686	At a water-to-material fixed ratio of 30 mL g-1, the highest yield of 3.72 ± 0.13% was obtained under the optimum conditions of extraction time of 32 min, extraction temperature at 47 °C, and extraction pressure at 1.87 Mpa, which were in agreement with the predicted value of 3.683%.
35519496	2	70	from	Mpa	624:626	arg1	temperature					570:580	extraction temperature	559:580	extraction temperature at 47 °C	559:589	At a water-to-material fixed ratio of 30 mL g-1, the highest yield of 3.72 ± 0.13% was obtained under the optimum conditions of extraction time of 32 min, extraction temperature at 47 °C, and extraction pressure at 1.87 Mpa, which were in agreement with the predicted value of 3.683%.
35519496	5	71	theme	oxide	1213:1217	arg1	release					1195:1201	the release	1191:1201	the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6)	1191:1281	In subsequent anti-inflammatory studies, when RAW 264.7 mouse macrophage cells were treated with TQCPS, satisfactory anti-inflammatory activity was observed, and TQCPS significantly suppressed the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) and synchronously restrained the expression levels of inducible nitric oxide synthase (iNOS), TNF-α, and IL-6 mRNA induced by lipopolysaccharide (LPS) in a dose-dependent manner.
35519496	3	72	theme	PAE	748:750	arg1	method					752:757	the PAE method	744:757	the PAE method	744:757	Compared with conventional hot water extraction (HWE), the PAE method remarkably enhanced the extraction yield with the further advantages of short extraction time and low extraction temperature.
35519496	0	73	theme	polysaccharides	102:116	arg1	activity					84:91	anti-inflammatory activity	66:91	anti-inflammatory activity	66:91	Optimization of the extraction, preliminary characterization, and anti-inflammatory activity of crude polysaccharides from the stems of Trapa quadrispinosa.
35519496	0	73	theme	polysaccharides	102:116	arg1	extraction					20:29	extraction	20:29	extraction	20:29	Optimization of the extraction, preliminary characterization, and anti-inflammatory activity of crude polysaccharides from the stems of Trapa quadrispinosa.
35519496	0	73	theme	polysaccharides	102:116	arg1	characterization					44:59	preliminary characterization	32:59	preliminary characterization	32:59	Optimization of the extraction, preliminary characterization, and anti-inflammatory activity of crude polysaccharides from the stems of Trapa quadrispinosa.
35519496	1	74	theme	response	368:375	arg1	RSM					398:400	RSM	398:400	RSM	398:400	A novel method was developed using pressurized-assisted extraction (PAE) to efficiently extract crude Trapa quadrispinosa polysaccharides (TQCPS) from plant stems, and the extraction process was optimized using response surface methodology (RSM).
35519496	1	74	theme	response	368:375	arg1	methodology					385:395	response surface methodology	368:395	response surface methodology (RSM)	368:401	A novel method was developed using pressurized-assisted extraction (PAE) to efficiently extract crude Trapa quadrispinosa polysaccharides (TQCPS) from plant stems, and the extraction process was optimized using response surface methodology (RSM).
35519496	3	75	theme	short	831:835	arg1	time					848:851	short extraction time	831:851	short extraction time	831:851	Compared with conventional hot water extraction (HWE), the PAE method remarkably enhanced the extraction yield with the further advantages of short extraction time and low extraction temperature.
35519496	2	76	theme	0.13	481:484	arg1	±					479:479	±	479:479	±	479:479	At a water-to-material fixed ratio of 30 mL g-1, the highest yield of 3.72 ± 0.13% was obtained under the optimum conditions of extraction time of 32 min, extraction temperature at 47 °C, and extraction pressure at 1.87 Mpa, which were in agreement with the predicted value of 3.683%.
35519496	5	77	theme	necrosis	1231:1238	arg1	TNF-α					1250:1254	TNF-α	1250:1254	TNF-α	1250:1254	In subsequent anti-inflammatory studies, when RAW 264.7 mouse macrophage cells were treated with TQCPS, satisfactory anti-inflammatory activity was observed, and TQCPS significantly suppressed the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) and synchronously restrained the expression levels of inducible nitric oxide synthase (iNOS), TNF-α, and IL-6 mRNA induced by lipopolysaccharide (LPS) in a dose-dependent manner.
35519496	5	77	theme	necrosis	1231:1238	arg1	factor-α					1240:1247	tumor necrosis factor-α	1225:1247	tumor necrosis factor-α (TNF-α)	1225:1255	In subsequent anti-inflammatory studies, when RAW 264.7 mouse macrophage cells were treated with TQCPS, satisfactory anti-inflammatory activity was observed, and TQCPS significantly suppressed the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) and synchronously restrained the expression levels of inducible nitric oxide synthase (iNOS), TNF-α, and IL-6 mRNA induced by lipopolysaccharide (LPS) in a dose-dependent manner.
35519496	0	78	theme	extraction	20:29	arg1	Optimization					0:11	Optimization	0:11	Optimization of the extraction, preliminary characterization, and anti-inflammatory activity of crude polysaccharides from the stems of Trapa quadrispinosa.	0:155	Optimization of the extraction, preliminary characterization, and anti-inflammatory activity of crude polysaccharides from the stems of Trapa quadrispinosa.
35519496	5	79	theme	anti-inflammatory	1115:1131	arg1	activity					1133:1140	satisfactory anti-inflammatory activity	1102:1140	satisfactory anti-inflammatory activity	1102:1140	In subsequent anti-inflammatory studies, when RAW 264.7 mouse macrophage cells were treated with TQCPS, satisfactory anti-inflammatory activity was observed, and TQCPS significantly suppressed the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) and synchronously restrained the expression levels of inducible nitric oxide synthase (iNOS), TNF-α, and IL-6 mRNA induced by lipopolysaccharide (LPS) in a dose-dependent manner.
35519496	3	80	theme	low	857:859	arg1	temperature					872:882	low extraction temperature	857:882	low extraction temperature	857:882	Compared with conventional hot water extraction (HWE), the PAE method remarkably enhanced the extraction yield with the further advantages of short extraction time and low extraction temperature.
35519496	2	81	theme	water-to-material	409:425	arg1	ratio					433:437	a water-to-material fixed ratio	407:437	a water-to-material fixed ratio of 30 mL g-1	407:450	At a water-to-material fixed ratio of 30 mL g-1, the highest yield of 3.72 ± 0.13% was obtained under the optimum conditions of extraction time of 32 min, extraction temperature at 47 °C, and extraction pressure at 1.87 Mpa, which were in agreement with the predicted value of 3.683%.
35519496	0	82	theme	preliminary	32:42	arg1	characterization					44:59	preliminary characterization	32:59	preliminary characterization	32:59	Optimization of the extraction, preliminary characterization, and anti-inflammatory activity of crude polysaccharides from the stems of Trapa quadrispinosa.
35519496	1	83	theme	plant	308:312	arg1	stems					314:318	plant stems	308:318	plant stems	308:318	A novel method was developed using pressurized-assisted extraction (PAE) to efficiently extract crude Trapa quadrispinosa polysaccharides (TQCPS) from plant stems, and the extraction process was optimized using response surface methodology (RSM).
35519496	3	84	theme	time	848:851	arg1	advantages					817:826	the further advantages	805:826	the further advantages of short extraction time and low extraction temperature	805:882	Compared with conventional hot water extraction (HWE), the PAE method remarkably enhanced the extraction yield with the further advantages of short extraction time and low extraction temperature.
35519496	6	85	from	extraction	1541:1550	arg1	plants					1586:1591	medicinal plants	1576:1591	medicinal plants	1576:1591	These results indicate that PAE is a technology that can be used for efficient extraction of polysaccharides from medicinal plants, and TQCPS can be explored as a potential anti-inflammatory agent in medicine.
35519496	5	86	theme	dose-dependent	1439:1452	arg1	manner					1454:1459	a dose-dependent manner	1437:1459	a dose-dependent manner	1437:1459	In subsequent anti-inflammatory studies, when RAW 264.7 mouse macrophage cells were treated with TQCPS, satisfactory anti-inflammatory activity was observed, and TQCPS significantly suppressed the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) and synchronously restrained the expression levels of inducible nitric oxide synthase (iNOS), TNF-α, and IL-6 mRNA induced by lipopolysaccharide (LPS) in a dose-dependent manner.
35519496	5	87	theme	RAW	1044:1046	arg1	cells					1071:1075	RAW 264.7 mouse macrophage cells	1044:1075	RAW 264.7 mouse macrophage cells	1044:1075	In subsequent anti-inflammatory studies, when RAW 264.7 mouse macrophage cells were treated with TQCPS, satisfactory anti-inflammatory activity was observed, and TQCPS significantly suppressed the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) and synchronously restrained the expression levels of inducible nitric oxide synthase (iNOS), TNF-α, and IL-6 mRNA induced by lipopolysaccharide (LPS) in a dose-dependent manner.
35519496	2	88	theme	g-1	448:450	arg1	ratio					433:437	a water-to-material fixed ratio	407:437	a water-to-material fixed ratio of 30 mL g-1	407:450	At a water-to-material fixed ratio of 30 mL g-1, the highest yield of 3.72 ± 0.13% was obtained under the optimum conditions of extraction time of 32 min, extraction temperature at 47 °C, and extraction pressure at 1.87 Mpa, which were in agreement with the predicted value of 3.683%.
35519496	2	89	from	°C	588:589	arg1	conditions					518:527	the optimum conditions	506:527	the optimum conditions of extraction time of 32 min, extraction temperature at 47 °C, and extraction pressure at 1.87 Mpa, which were in agreement with the predicted value of 3.683%	506:686	At a water-to-material fixed ratio of 30 mL g-1, the highest yield of 3.72 ± 0.13% was obtained under the optimum conditions of extraction time of 32 min, extraction temperature at 47 °C, and extraction pressure at 1.87 Mpa, which were in agreement with the predicted value of 3.683%.
35519496	2	89	from	°C	588:589	arg1	time					543:546	extraction time	532:546	extraction time of 32 min	532:556	At a water-to-material fixed ratio of 30 mL g-1, the highest yield of 3.72 ± 0.13% was obtained under the optimum conditions of extraction time of 32 min, extraction temperature at 47 °C, and extraction pressure at 1.87 Mpa, which were in agreement with the predicted value of 3.683%.
35519496	2	89	from	°C	588:589	arg1	pressure					607:614	extraction pressure	596:614	extraction pressure at 1.87 Mpa, which were in agreement with the predicted value of 3.683%	596:686	At a water-to-material fixed ratio of 30 mL g-1, the highest yield of 3.72 ± 0.13% was obtained under the optimum conditions of extraction time of 32 min, extraction temperature at 47 °C, and extraction pressure at 1.87 Mpa, which were in agreement with the predicted value of 3.683%.
35519496	2	89	from	°C	588:589	arg1	temperature					570:580	extraction temperature	559:580	extraction temperature at 47 °C	559:589	At a water-to-material fixed ratio of 30 mL g-1, the highest yield of 3.72 ± 0.13% was obtained under the optimum conditions of extraction time of 32 min, extraction temperature at 47 °C, and extraction pressure at 1.87 Mpa, which were in agreement with the predicted value of 3.683%.
35519496	3	90	theme	temperature	872:882	arg1	advantages					817:826	the further advantages	805:826	the further advantages of short extraction time and low extraction temperature	805:882	Compared with conventional hot water extraction (HWE), the PAE method remarkably enhanced the extraction yield with the further advantages of short extraction time and low extraction temperature.
35519496	2	91	from	conditions	518:527	arg1	Mpa					624:626	1.87 Mpa	619:626	1.87 Mpa	619:626	At a water-to-material fixed ratio of 30 mL g-1, the highest yield of 3.72 ± 0.13% was obtained under the optimum conditions of extraction time of 32 min, extraction temperature at 47 °C, and extraction pressure at 1.87 Mpa, which were in agreement with the predicted value of 3.683%.
35519496	2	91	from	conditions	518:527	arg1	°C					588:589	47 °C	585:589	47 °C	585:589	At a water-to-material fixed ratio of 30 mL g-1, the highest yield of 3.72 ± 0.13% was obtained under the optimum conditions of extraction time of 32 min, extraction temperature at 47 °C, and extraction pressure at 1.87 Mpa, which were in agreement with the predicted value of 3.683%.
35519496	6	92	from	plants	1586:1591	arg1	polysaccharides					1555:1569	polysaccharides	1555:1569	polysaccharides from medicinal plants	1555:1591	These results indicate that PAE is a technology that can be used for efficient extraction of polysaccharides from medicinal plants, and TQCPS can be explored as a potential anti-inflammatory agent in medicine.
35519496	6	92	from	plants	1586:1591	arg1	extraction					1541:1550	efficient extraction	1531:1550	efficient extraction of polysaccharides from medicinal plants	1531:1591	These results indicate that PAE is a technology that can be used for efficient extraction of polysaccharides from medicinal plants, and TQCPS can be explored as a potential anti-inflammatory agent in medicine.
35519496	1	93	theme	crude	253:257	arg1	TQCPS					296:300	TQCPS	296:300	TQCPS	296:300	A novel method was developed using pressurized-assisted extraction (PAE) to efficiently extract crude Trapa quadrispinosa polysaccharides (TQCPS) from plant stems, and the extraction process was optimized using response surface methodology (RSM).
35519496	1	93	theme	crude	253:257	arg1	polysaccharides					279:293	crude Trapa quadrispinosa polysaccharides	253:293	crude Trapa quadrispinosa polysaccharides (TQCPS)	253:301	A novel method was developed using pressurized-assisted extraction (PAE) to efficiently extract crude Trapa quadrispinosa polysaccharides (TQCPS) from plant stems, and the extraction process was optimized using response surface methodology (RSM).
30483450	0	0	theme	lucidum	97:103	arg1	spores					77:82	spores	77:82	spores of Ganoderma lucidum	77:103	Supercritical fluid extraction effectively removes phthalate plasticizers in spores of Ganoderma lucidum.
30483450	6	1	contain	had	813:815	arg2	increase					825:832	a 7.45% increase	817:832	a 7.45% increase	817:832	However, the triterpenoid extracts of SFE spores had a 7.45% increase, significantly higher than that in control spores.
30483450	6	1	contain	had	813:815	arg1	extracts					790:797	the triterpenoid extracts	773:797	the triterpenoid extracts of SFE spores	773:811	However, the triterpenoid extracts of SFE spores had a 7.45% increase, significantly higher than that in control spores.
30483450	8	2	theme	related	1062:1068	arg1	products					1070:1077	food related products	1057:1077	food related products	1057:1077	Our results suggested SFE is a potential approach to remove phthalate from food related products.
30483450	4	3	theme	di-iso-butyl	533:544	arg1	phthalate					568:576	the residual di-iso-butyl phthalate, di-n-butyl phthalate	520:576	phthalate	568:576	The results showed SFE removed 100% of the residual di-iso-butyl phthalate, di-n-butyl phthalate and di-2-ethylhexyl phthalate in the spores of G. lucidum.
30483450	0	4	theme	Ganoderma	87:95	arg1	lucidum					97:103	Ganoderma lucidum	87:103	Ganoderma lucidum	87:103	Supercritical fluid extraction effectively removes phthalate plasticizers in spores of Ganoderma lucidum.
30483450	4	5	dep	showed	493:498	arg1	removed					504:510	removed	504:510	showed SFE removed 100% of the residual di-iso-butyl phthalate, di-n-butyl phthalate and di-2-ethylhexyl phthalate in the spores of G. lucidum	493:634	The results showed SFE removed 100% of the residual di-iso-butyl phthalate, di-n-butyl phthalate and di-2-ethylhexyl phthalate in the spores of G. lucidum.
30483450	6	6	theme	spores	806:811	arg1	extracts					790:797	the triterpenoid extracts	773:797	the triterpenoid extracts of SFE spores	773:811	However, the triterpenoid extracts of SFE spores had a 7.45% increase, significantly higher than that in control spores.
30483450	1	7	theme	public	168:173	arg1	health					175:180	public health	168:180	public health	168:180	Phthalate plasticizers residue in food is a serious threat to public health.
30483450	4	8	theme	residual	524:531	arg1	phthalate					568:576	the residual di-iso-butyl phthalate, di-n-butyl phthalate	520:576	phthalate	568:576	The results showed SFE removed 100% of the residual di-iso-butyl phthalate, di-n-butyl phthalate and di-2-ethylhexyl phthalate in the spores of G. lucidum.
30483450	5	9	theme	acid	701:704	arg1	composition					706:716	fatty acid composition	695:716	fatty acid composition	695:716	No significant differences in polysaccharides content and fatty acid composition were observed between SFE and control spores.
30483450	8	10	theme	potential	1013:1021	arg1	SFE					1004:1006	SFE	1004:1006	SFE	1004:1006	Our results suggested SFE is a potential approach to remove phthalate from food related products.
30483450	8	10	theme	potential	1013:1021	arg1	approach					1023:1030	a potential approach	1011:1030	a potential approach to remove phthalate from food related products	1011:1077	Our results suggested SFE is a potential approach to remove phthalate from food related products.
30483450	3	11	from	phthalates	365:374	arg1	spores					379:384	spores	379:384	spores of G. lucidum	379:398	In this study, supercritical fluid extraction (SFE) was performed to remove phthalates in spores of G. lucidum, and the effects on acid and peroxide values of spores' oil were also evaluated.
30483450	6	12	theme	triterpenoid	777:788	arg1	extracts					790:797	the triterpenoid extracts	773:797	the triterpenoid extracts of SFE spores	773:811	However, the triterpenoid extracts of SFE spores had a 7.45% increase, significantly higher than that in control spores.
30483450	4	13	theme	phthalate	546:554	arg1	phthalate					568:576	the residual di-iso-butyl phthalate, di-n-butyl phthalate	520:576	phthalate	568:576	The results showed SFE removed 100% of the residual di-iso-butyl phthalate, di-n-butyl phthalate and di-2-ethylhexyl phthalate in the spores of G. lucidum.
30483450	4	14	theme	G.	625:626	arg1	lucidum					628:634	G. lucidum	625:634	G. lucidum	625:634	The results showed SFE removed 100% of the residual di-iso-butyl phthalate, di-n-butyl phthalate and di-2-ethylhexyl phthalate in the spores of G. lucidum.
30483450	6	15	theme	higher	849:854	arg1	increase					825:832	a 7.45% increase	817:832	a 7.45% increase	817:832	However, the triterpenoid extracts of SFE spores had a 7.45% increase, significantly higher than that in control spores.
30483450	3	16	theme	acid	420:423	arg1	values					438:443	acid and peroxide values	420:443	acid and peroxide values of spores' oil	420:458	In this study, supercritical fluid extraction (SFE) was performed to remove phthalates in spores of G. lucidum, and the effects on acid and peroxide values of spores' oil were also evaluated.
30483450	0	17	theme	fluid	14:18	arg1	extraction					20:29	Supercritical fluid extraction	0:29	Supercritical fluid extraction	0:29	Supercritical fluid extraction effectively removes phthalate plasticizers in spores of Ganoderma lucidum.
30483450	5	18	theme	significant	640:650	arg1	differences					652:662	No significant differences	637:662	No significant differences in polysaccharides content and fatty acid composition	637:716	No significant differences in polysaccharides content and fatty acid composition were observed between SFE and control spores.
30483450	0	19	theme	Supercritical	0:12	arg1	extraction					20:29	Supercritical fluid extraction	0:29	Supercritical fluid extraction	0:29	Supercritical fluid extraction effectively removes phthalate plasticizers in spores of Ganoderma lucidum.
30483450	8	20	theme	food	1057:1060	arg1	products					1070:1077	food related products	1057:1077	food related products	1057:1077	Our results suggested SFE is a potential approach to remove phthalate from food related products.
30483450	6	21	theme	SFE	802:804	arg1	spores					806:811	SFE spores	802:811	SFE spores	802:811	However, the triterpenoid extracts of SFE spores had a 7.45% increase, significantly higher than that in control spores.
30483450	7	22	theme	Accelerated	885:895	arg1	tests					907:911	Accelerated oxidation tests	885:911	Accelerated oxidation tests	885:911	Accelerated oxidation tests further implied that SFE could improve the stability of spores' oil.
30483450	1	23	theme	Phthalate	106:114	arg1	plasticizers					116:127	Phthalate plasticizers	106:127	Phthalate plasticizers residue in food	106:143	Phthalate plasticizers residue in food is a serious threat to public health.
30483450	4	24	theme	lucidum	628:634	arg1	spores					615:620	the spores	611:620	the spores of G. lucidum	611:634	The results showed SFE removed 100% of the residual di-iso-butyl phthalate, di-n-butyl phthalate and di-2-ethylhexyl phthalate in the spores of G. lucidum.
30483450	4	25	from	%	515:515	arg1	spores					615:620	the spores	611:620	the spores of G. lucidum	611:634	The results showed SFE removed 100% of the residual di-iso-butyl phthalate, di-n-butyl phthalate and di-2-ethylhexyl phthalate in the spores of G. lucidum.
30483450	5	26	theme	polysaccharides	667:681	arg1	content					683:689	polysaccharides content	667:689	polysaccharides content	667:689	No significant differences in polysaccharides content and fatty acid composition were observed between SFE and control spores.
30483450	7	27	theme	oxidation	897:905	arg1	tests					907:911	Accelerated oxidation tests	885:911	Accelerated oxidation tests	885:911	Accelerated oxidation tests further implied that SFE could improve the stability of spores' oil.
30483450	1	28	theme	plasticizers	116:127	arg1	threat					158:163	a serious threat	148:163	a serious threat to public health	148:180	Phthalate plasticizers residue in food is a serious threat to public health.
30483450	1	28	theme	plasticizers	116:127	arg1	residue					129:135	Phthalate plasticizers residue	106:135	Phthalate plasticizers residue in food	106:143	Phthalate plasticizers residue in food is a serious threat to public health.
30483450	0	29	theme	phthalate	51:59	arg1	plasticizers					61:72	phthalate plasticizers	51:72	phthalate plasticizers	51:72	Supercritical fluid extraction effectively removes phthalate plasticizers in spores of Ganoderma lucidum.
30483450	4	30	from	phthalate	598:606	arg1	spores					615:620	the spores	611:620	the spores of G. lucidum	611:634	The results showed SFE removed 100% of the residual di-iso-butyl phthalate, di-n-butyl phthalate and di-2-ethylhexyl phthalate in the spores of G. lucidum.
30483450	5	31	theme	fatty	695:699	arg1	composition					706:716	fatty acid composition	695:716	fatty acid composition	695:716	No significant differences in polysaccharides content and fatty acid composition were observed between SFE and control spores.
30483450	6	32	theme	%	823:823	arg1	increase					825:832	a 7.45% increase	817:832	a 7.45% increase	817:832	However, the triterpenoid extracts of SFE spores had a 7.45% increase, significantly higher than that in control spores.
30483450	3	33	theme	peroxide	429:436	arg1	values					438:443	acid and peroxide values	420:443	acid and peroxide values of spores' oil	420:458	In this study, supercritical fluid extraction (SFE) was performed to remove phthalates in spores of G. lucidum, and the effects on acid and peroxide values of spores' oil were also evaluated.
30483450	3	34	theme	G.	389:390	arg1	lucidum					392:398	G. lucidum	389:398	G. lucidum	389:398	In this study, supercritical fluid extraction (SFE) was performed to remove phthalates in spores of G. lucidum, and the effects on acid and peroxide values of spores' oil were also evaluated.
30483450	3	35	theme	supercritical	304:316	arg1	SFE					336:338	SFE	336:338	SFE	336:338	In this study, supercritical fluid extraction (SFE) was performed to remove phthalates in spores of G. lucidum, and the effects on acid and peroxide values of spores' oil were also evaluated.
30483450	3	35	theme	supercritical	304:316	arg1	extraction					324:333	supercritical fluid extraction	304:333	supercritical fluid extraction (SFE)	304:339	In this study, supercritical fluid extraction (SFE) was performed to remove phthalates in spores of G. lucidum, and the effects on acid and peroxide values of spores' oil were also evaluated.
30483450	6	36	theme	7.45	819:822	arg1	%					823:823	%	823:823	%	823:823	However, the triterpenoid extracts of SFE spores had a 7.45% increase, significantly higher than that in control spores.
30483450	3	37	theme	oil	456:458	arg1	values					438:443	acid and peroxide values	420:443	acid and peroxide values of spores' oil	420:458	In this study, supercritical fluid extraction (SFE) was performed to remove phthalates in spores of G. lucidum, and the effects on acid and peroxide values of spores' oil were also evaluated.
30483450	3	38	theme	lucidum	392:398	arg1	spores					379:384	spores	379:384	spores of G. lucidum	379:398	In this study, supercritical fluid extraction (SFE) was performed to remove phthalates in spores of G. lucidum, and the effects on acid and peroxide values of spores' oil were also evaluated.
30483450	3	39	theme	fluid	318:322	arg1	SFE					336:338	SFE	336:338	SFE	336:338	In this study, supercritical fluid extraction (SFE) was performed to remove phthalates in spores of G. lucidum, and the effects on acid and peroxide values of spores' oil were also evaluated.
30483450	3	39	theme	fluid	318:322	arg1	extraction					324:333	supercritical fluid extraction	304:333	supercritical fluid extraction (SFE)	304:339	In this study, supercritical fluid extraction (SFE) was performed to remove phthalates in spores of G. lucidum, and the effects on acid and peroxide values of spores' oil were also evaluated.
30483450	6	40	theme	control	869:875	arg1	spores					877:882	control spores	869:882	control spores	869:882	However, the triterpenoid extracts of SFE spores had a 7.45% increase, significantly higher than that in control spores.
30483450	8	41	attach	remove	1035:1040	arg2	approach					1023:1030	a potential approach	1011:1030	a potential approach to remove phthalate from food related products	1011:1077	Our results suggested SFE is a potential approach to remove phthalate from food related products.
30483450	8	41	attach	remove	1035:1040	arg2	SFE					1004:1006	SFE	1004:1006	SFE	1004:1006	Our results suggested SFE is a potential approach to remove phthalate from food related products.
30483450	8	41	attach	remove	1035:1040	arg1	products					1070:1077	food related products	1057:1077	food related products	1057:1077	Our results suggested SFE is a potential approach to remove phthalate from food related products.
30483450	5	42	dep	SFE	740:742	arg1	spores					756:761	spores	756:761	spores	756:761	No significant differences in polysaccharides content and fatty acid composition were observed between SFE and control spores.
30483450	1	43	from	residue	129:135	arg1	food					140:143	food	140:143	food	140:143	Phthalate plasticizers residue in food is a serious threat to public health.
30483450	4	44	theme	phthalate	568:576	arg1	%					515:515	100%	512:515	100% of the residual di-iso-butyl phthalate, di-n-butyl phthalate	512:576	The results showed SFE removed 100% of the residual di-iso-butyl phthalate, di-n-butyl phthalate and di-2-ethylhexyl phthalate in the spores of G. lucidum.
30483450	4	44	theme	phthalate	568:576	arg1	phthalate					598:606	di-2-ethylhexyl phthalate	582:606	di-2-ethylhexyl phthalate in the spores of G. lucidum	582:634	The results showed SFE removed 100% of the residual di-iso-butyl phthalate, di-n-butyl phthalate and di-2-ethylhexyl phthalate in the spores of G. lucidum.
30483450	4	44	theme	phthalate	568:576	arg1	phthalate					568:576	the residual di-iso-butyl phthalate, di-n-butyl phthalate	520:576	phthalate	568:576	The results showed SFE removed 100% of the residual di-iso-butyl phthalate, di-n-butyl phthalate and di-2-ethylhexyl phthalate in the spores of G. lucidum.
30483450	2	45	theme	lucidum	203:209	arg1	Spores					183:188	Spores	183:188	Spores of Ganoderma lucidum	183:209	Spores of Ganoderma lucidum are easy to be contaminated with phthalates during collection and processing.
30483450	4	46	theme	di-n-butyl	557:566	arg1	phthalate					568:576	the residual di-iso-butyl phthalate, di-n-butyl phthalate	520:576	phthalate	568:576	The results showed SFE removed 100% of the residual di-iso-butyl phthalate, di-n-butyl phthalate and di-2-ethylhexyl phthalate in the spores of G. lucidum.
30483450	2	47	theme	Ganoderma	193:201	arg1	lucidum					203:209	Ganoderma lucidum	193:209	Ganoderma lucidum	193:209	Spores of Ganoderma lucidum are easy to be contaminated with phthalates during collection and processing.
30483450	3	48	from	effects	409:415	arg1	values					438:443	acid and peroxide values	420:443	acid and peroxide values of spores' oil	420:458	In this study, supercritical fluid extraction (SFE) was performed to remove phthalates in spores of G. lucidum, and the effects on acid and peroxide values of spores' oil were also evaluated.
30483450	1	49	theme	serious	150:156	arg1	threat					158:163	a serious threat	148:163	a serious threat to public health	148:180	Phthalate plasticizers residue in food is a serious threat to public health.
30483450	1	49	theme	serious	150:156	arg1	residue					129:135	Phthalate plasticizers residue	106:135	Phthalate plasticizers residue in food	106:143	Phthalate plasticizers residue in food is a serious threat to public health.
30483450	4	50	theme	di-2-ethylhexyl	582:596	arg1	phthalate					598:606	di-2-ethylhexyl phthalate	582:606	di-2-ethylhexyl phthalate in the spores of G. lucidum	582:634	The results showed SFE removed 100% of the residual di-iso-butyl phthalate, di-n-butyl phthalate and di-2-ethylhexyl phthalate in the spores of G. lucidum.
30483450	5	51	from	differences	652:662	arg1	content					683:689	polysaccharides content	667:689	polysaccharides content	667:689	No significant differences in polysaccharides content and fatty acid composition were observed between SFE and control spores.
30483450	5	51	from	differences	652:662	arg1	composition					706:716	fatty acid composition	695:716	fatty acid composition	695:716	No significant differences in polysaccharides content and fatty acid composition were observed between SFE and control spores.
30561033	0	0	theme	gut	92:94	arg1	microbiome					96:105	gut microbiome	92:105	gut microbiome	92:105	Synbindin deficiency inhibits colon carcinogenesis by attenuating Wnt cascade and balancing gut microbiome.
30561033	4	1	theme	colitis-associated	529:546	arg1	model					561:565	the colitis-associated cancer (CAC) model	525:565	the colitis-associated cancer (CAC) model	525:565	Intestine-specific disruption of Synbindin balanced the disturbed gut microbiota and protected mice against tumor formation in the colitis-associated cancer (CAC) model.
30561033	9	2	from	activation	1257:1266	arg1	carcinogenesis					1282:1295	colorectal carcinogenesis	1271:1295	colorectal carcinogenesis	1271:1295	These findings identify a critical role of Synbindin in gut microbiome composition and Wnt signaling activation in colorectal carcinogenesis, and highlight Synbindin as an adaptor protein with multifaceted roles.
30561033	9	3	theme	signaling	1247:1255	arg1	activation					1257:1266	Wnt signaling activation	1243:1266	Wnt signaling activation	1243:1266	These findings identify a critical role of Synbindin in gut microbiome composition and Wnt signaling activation in colorectal carcinogenesis, and highlight Synbindin as an adaptor protein with multifaceted roles.
30561033	1	4	theme	colorectal	165:174	arg1	CRC					184:186	CRC	184:186	CRC	184:186	The molecular mechanisms that control the development of colorectal cancer (CRC) remain poorly defined.
30561033	1	4	theme	colorectal	165:174	arg1	cancer					176:181	colorectal cancer	165:181	colorectal cancer (CRC)	165:187	The molecular mechanisms that control the development of colorectal cancer (CRC) remain poorly defined.
30561033	7	5	dep	signaling	863:871	arg1	node					892:895	a major regulatory node	873:895	Wnt signaling a major regulatory node downstream of Synbindin	859:919	Further transcriptomic sequencing identified Wnt signaling a major regulatory node downstream of Synbindin.
30561033	4	6	theme	CAC	556:558	arg1	model					561:565	the colitis-associated cancer (CAC) model	525:565	the colitis-associated cancer (CAC) model	525:565	Intestine-specific disruption of Synbindin balanced the disturbed gut microbiota and protected mice against tumor formation in the colitis-associated cancer (CAC) model.
30561033	8	7	theme	signaling	1145:1153	arg1	intensity					1128:1136	the intensity	1124:1136	the intensity of Wnt signaling	1124:1153	Combined molecular and cellular characterizations revealed that Synbindin confers Disheveled-3 (DVL3)-based signalosome assembly and acts as a modular scaffold for DVL3 and Axin2 complex, orchestrating the intensity of Wnt signaling.
30561033	1	8	theme	cancer	176:181	arg1	development					150:160	the development	146:160	the development of colorectal cancer (CRC)	146:187	The molecular mechanisms that control the development of colorectal cancer (CRC) remain poorly defined.
30561033	6	9	theme	goblet	655:660	arg1	cells					662:666	increased goblet cells	645:666	increased goblet cells	645:666	In host, increased goblet cells and mucin2 expression, together with increased intestinal epithelial cells (IECs) apoptosis and decreased epithelial proliferation were observed.
30561033	2	10	theme	gut	301:303	arg1	microbiome					305:314	gut microbiome	301:314	gut microbiome	301:314	Here we show Synbindin promoted CRC oncogenesis by activating Wnt signaling and altering gut microbiome.
30561033	3	11	theme	poor	374:377	arg1	prognosis					387:395	poor patient prognosis	374:395	poor patient prognosis	374:395	Synbindin upregulation in human CRCs was associated with poor patient prognosis.
30561033	9	12	theme	colorectal	1271:1280	arg1	carcinogenesis					1282:1295	colorectal carcinogenesis	1271:1295	colorectal carcinogenesis	1271:1295	These findings identify a critical role of Synbindin in gut microbiome composition and Wnt signaling activation in colorectal carcinogenesis, and highlight Synbindin as an adaptor protein with multifaceted roles.
30561033	7	13	theme	regulatory	881:890	arg1	node					892:895	a major regulatory node	873:895	Wnt signaling a major regulatory node downstream of Synbindin	859:919	Further transcriptomic sequencing identified Wnt signaling a major regulatory node downstream of Synbindin.
30561033	8	14	theme	cellular	945:952	arg1	characterizations					954:970	Combined molecular and cellular characterizations	922:970	Combined molecular and cellular characterizations	922:970	Combined molecular and cellular characterizations revealed that Synbindin confers Disheveled-3 (DVL3)-based signalosome assembly and acts as a modular scaffold for DVL3 and Axin2 complex, orchestrating the intensity of Wnt signaling.
30561033	4	15	theme	tumor	506:510	arg1	formation					512:520	tumor formation	506:520	tumor formation in the colitis-associated cancer (CAC) model	506:565	Intestine-specific disruption of Synbindin balanced the disturbed gut microbiota and protected mice against tumor formation in the colitis-associated cancer (CAC) model.
30561033	4	16	theme	Synbindin	431:439	arg1	disruption					417:426	Intestine-specific disruption	398:426	Intestine-specific disruption of Synbindin	398:439	Intestine-specific disruption of Synbindin balanced the disturbed gut microbiota and protected mice against tumor formation in the colitis-associated cancer (CAC) model.
30561033	2	17	theme	Wnt	274:276	arg1	signaling					278:286	Wnt signaling	274:286	Wnt signaling	274:286	Here we show Synbindin promoted CRC oncogenesis by activating Wnt signaling and altering gut microbiome.
30561033	7	18	theme	Synbindin	911:919	arg1	downstream					897:906	downstream	897:906	downstream	897:906	Further transcriptomic sequencing identified Wnt signaling a major regulatory node downstream of Synbindin.
30561033	4	19	from	formation	512:520	arg1	model					561:565	the colitis-associated cancer (CAC) model	525:565	the colitis-associated cancer (CAC) model	525:565	Intestine-specific disruption of Synbindin balanced the disturbed gut microbiota and protected mice against tumor formation in the colitis-associated cancer (CAC) model.
30561033	5	20	theme	gut	610:612	arg1	depletion					625:633	gut microbiota depletion	610:633	gut microbiota depletion	610:633	The protective role was compromised after gut microbiota depletion.
30561033	9	21	from	composition	1227:1237	arg1	carcinogenesis					1282:1295	colorectal carcinogenesis	1271:1295	colorectal carcinogenesis	1271:1295	These findings identify a critical role of Synbindin in gut microbiome composition and Wnt signaling activation in colorectal carcinogenesis, and highlight Synbindin as an adaptor protein with multifaceted roles.
30561033	0	22	theme	Synbindin	0:8	arg1	deficiency					10:19	Synbindin deficiency	0:19	Synbindin deficiency	0:19	Synbindin deficiency inhibits colon carcinogenesis by attenuating Wnt cascade and balancing gut microbiome.
30561033	8	23	theme	Wnt	1141:1143	arg1	signaling					1145:1153	Wnt signaling	1141:1153	Wnt signaling	1141:1153	Combined molecular and cellular characterizations revealed that Synbindin confers Disheveled-3 (DVL3)-based signalosome assembly and acts as a modular scaffold for DVL3 and Axin2 complex, orchestrating the intensity of Wnt signaling.
30561033	4	24	theme	Intestine-specific	398:415	arg1	disruption					417:426	Intestine-specific disruption	398:426	Intestine-specific disruption of Synbindin	398:439	Intestine-specific disruption of Synbindin balanced the disturbed gut microbiota and protected mice against tumor formation in the colitis-associated cancer (CAC) model.
30561033	3	25	from	upregulation	327:338	arg1	CRCs					349:352	human CRCs	343:352	human CRCs	343:352	Synbindin upregulation in human CRCs was associated with poor patient prognosis.
30561033	6	26	theme	epithelial	726:735	arg1	IECs					744:747	IECs	744:747	IECs	744:747	In host, increased goblet cells and mucin2 expression, together with increased intestinal epithelial cells (IECs) apoptosis and decreased epithelial proliferation were observed.
30561033	6	26	theme	epithelial	726:735	arg1	cells					737:741	increased intestinal epithelial cells	705:741	increased intestinal epithelial cells (IECs)	705:748	In host, increased goblet cells and mucin2 expression, together with increased intestinal epithelial cells (IECs) apoptosis and decreased epithelial proliferation were observed.
30561033	8	27	theme	Axin2	1095:1099	arg1	complex					1101:1107	Axin2 complex	1095:1107	Axin2 complex	1095:1107	Combined molecular and cellular characterizations revealed that Synbindin confers Disheveled-3 (DVL3)-based signalosome assembly and acts as a modular scaffold for DVL3 and Axin2 complex, orchestrating the intensity of Wnt signaling.
30561033	0	28	theme	colon	30:34	arg1	carcinogenesis					36:49	colon carcinogenesis	30:49	colon carcinogenesis	30:49	Synbindin deficiency inhibits colon carcinogenesis by attenuating Wnt cascade and balancing gut microbiome.
30561033	5	29	theme	microbiota	614:623	arg1	depletion					625:633	gut microbiota depletion	610:633	gut microbiota depletion	610:633	The protective role was compromised after gut microbiota depletion.
30561033	8	30	theme	modular	1065:1071	arg1	Synbindin					986:994	Synbindin	986:994	Synbindin	986:994	Combined molecular and cellular characterizations revealed that Synbindin confers Disheveled-3 (DVL3)-based signalosome assembly and acts as a modular scaffold for DVL3 and Axin2 complex, orchestrating the intensity of Wnt signaling.
30561033	8	30	theme	modular	1065:1071	arg1	scaffold					1073:1080	a modular scaffold	1063:1080	a modular scaffold for DVL3 and Axin2 complex	1063:1107	Combined molecular and cellular characterizations revealed that Synbindin confers Disheveled-3 (DVL3)-based signalosome assembly and acts as a modular scaffold for DVL3 and Axin2 complex, orchestrating the intensity of Wnt signaling.
30561033	6	31	theme	intestinal	715:724	arg1	IECs					744:747	IECs	744:747	IECs	744:747	In host, increased goblet cells and mucin2 expression, together with increased intestinal epithelial cells (IECs) apoptosis and decreased epithelial proliferation were observed.
30561033	6	31	theme	intestinal	715:724	arg1	cells					737:741	increased intestinal epithelial cells	705:741	increased intestinal epithelial cells (IECs)	705:748	In host, increased goblet cells and mucin2 expression, together with increased intestinal epithelial cells (IECs) apoptosis and decreased epithelial proliferation were observed.
30561033	8	32	theme	signalosome	1030:1040	arg1	assembly					1042:1049	Disheveled-3 (DVL3)-based signalosome assembly	1004:1049	Disheveled-3 (DVL3)-based signalosome assembly	1004:1049	Combined molecular and cellular characterizations revealed that Synbindin confers Disheveled-3 (DVL3)-based signalosome assembly and acts as a modular scaffold for DVL3 and Axin2 complex, orchestrating the intensity of Wnt signaling.
30561033	7	33	theme	Wnt	859:861	arg1	signaling					863:871	Wnt signaling a major regulatory node downstream of Synbindin	859:919	Wnt signaling a major regulatory node downstream of Synbindin	859:919	Further transcriptomic sequencing identified Wnt signaling a major regulatory node downstream of Synbindin.
30561033	9	34	theme	gut	1212:1214	arg1	composition					1227:1237	gut microbiome composition	1212:1237	gut microbiome composition	1212:1237	These findings identify a critical role of Synbindin in gut microbiome composition and Wnt signaling activation in colorectal carcinogenesis, and highlight Synbindin as an adaptor protein with multifaceted roles.
30561033	1	35	theme	molecular	112:120	arg1	mechanisms					122:131	The molecular mechanisms	108:131	The molecular mechanisms that control the development of colorectal cancer (CRC)	108:187	The molecular mechanisms that control the development of colorectal cancer (CRC) remain poorly defined.
30561033	6	36	theme	increased	705:713	arg1	IECs					744:747	IECs	744:747	IECs	744:747	In host, increased goblet cells and mucin2 expression, together with increased intestinal epithelial cells (IECs) apoptosis and decreased epithelial proliferation were observed.
30561033	6	36	theme	increased	705:713	arg1	cells					737:741	increased intestinal epithelial cells	705:741	increased intestinal epithelial cells (IECs)	705:748	In host, increased goblet cells and mucin2 expression, together with increased intestinal epithelial cells (IECs) apoptosis and decreased epithelial proliferation were observed.
30561033	6	37	located	observed	804:811	arg1	cells					662:666	increased goblet cells	645:666	increased goblet cells	645:666	In host, increased goblet cells and mucin2 expression, together with increased intestinal epithelial cells (IECs) apoptosis and decreased epithelial proliferation were observed.
30561033	6	37	located	observed	804:811	arg2	apoptosis					750:758	apoptosis	750:758	apoptosis	750:758	In host, increased goblet cells and mucin2 expression, together with increased intestinal epithelial cells (IECs) apoptosis and decreased epithelial proliferation were observed.
30561033	6	37	located	observed	804:811	arg1	host					639:642	host	639:642	host	639:642	In host, increased goblet cells and mucin2 expression, together with increased intestinal epithelial cells (IECs) apoptosis and decreased epithelial proliferation were observed.
30561033	6	37	located	observed	804:811	arg2	proliferation					785:797	decreased epithelial proliferation	764:797	decreased epithelial proliferation	764:797	In host, increased goblet cells and mucin2 expression, together with increased intestinal epithelial cells (IECs) apoptosis and decreased epithelial proliferation were observed.
30561033	6	37	located	observed	804:811	arg1	expression					679:688	mucin2 expression	672:688	mucin2 expression	672:688	In host, increased goblet cells and mucin2 expression, together with increased intestinal epithelial cells (IECs) apoptosis and decreased epithelial proliferation were observed.
30561033	8	38	theme	molecular	931:939	arg1	characterizations					954:970	Combined molecular and cellular characterizations	922:970	Combined molecular and cellular characterizations	922:970	Combined molecular and cellular characterizations revealed that Synbindin confers Disheveled-3 (DVL3)-based signalosome assembly and acts as a modular scaffold for DVL3 and Axin2 complex, orchestrating the intensity of Wnt signaling.
30561033	9	39	from	role	1191:1194	arg1	activation					1257:1266	Wnt signaling activation	1243:1266	Wnt signaling activation	1243:1266	These findings identify a critical role of Synbindin in gut microbiome composition and Wnt signaling activation in colorectal carcinogenesis, and highlight Synbindin as an adaptor protein with multifaceted roles.
30561033	9	39	from	role	1191:1194	arg1	composition					1227:1237	gut microbiome composition	1212:1237	gut microbiome composition	1212:1237	These findings identify a critical role of Synbindin in gut microbiome composition and Wnt signaling activation in colorectal carcinogenesis, and highlight Synbindin as an adaptor protein with multifaceted roles.
30561033	9	40	theme	adaptor	1328:1334	arg1	protein					1336:1342	an adaptor protein	1325:1342	an adaptor protein with multifaceted roles	1325:1366	These findings identify a critical role of Synbindin in gut microbiome composition and Wnt signaling activation in colorectal carcinogenesis, and highlight Synbindin as an adaptor protein with multifaceted roles.
30561033	9	40	theme	adaptor	1328:1334	arg1	Synbindin					1312:1320	Synbindin	1312:1320	Synbindin	1312:1320	These findings identify a critical role of Synbindin in gut microbiome composition and Wnt signaling activation in colorectal carcinogenesis, and highlight Synbindin as an adaptor protein with multifaceted roles.
30561033	3	41	theme	patient	379:385	arg1	prognosis					387:395	poor patient prognosis	374:395	poor patient prognosis	374:395	Synbindin upregulation in human CRCs was associated with poor patient prognosis.
30561033	5	42	theme	protective	572:581	arg1	role					583:586	The protective role	568:586	The protective role	568:586	The protective role was compromised after gut microbiota depletion.
30561033	0	43	theme	Wnt	66:68	arg1	cascade					70:76	Wnt cascade	66:76	Wnt cascade	66:76	Synbindin deficiency inhibits colon carcinogenesis by attenuating Wnt cascade and balancing gut microbiome.
30561033	7	44	theme	major	875:879	arg1	node					892:895	a major regulatory node	873:895	Wnt signaling a major regulatory node downstream of Synbindin	859:919	Further transcriptomic sequencing identified Wnt signaling a major regulatory node downstream of Synbindin.
30561033	8	45	theme	Combined	922:929	arg1	characterizations					954:970	Combined molecular and cellular characterizations	922:970	Combined molecular and cellular characterizations	922:970	Combined molecular and cellular characterizations revealed that Synbindin confers Disheveled-3 (DVL3)-based signalosome assembly and acts as a modular scaffold for DVL3 and Axin2 complex, orchestrating the intensity of Wnt signaling.
30561033	7	46	theme	Further	814:820	arg1	sequencing					837:846	Further transcriptomic sequencing	814:846	Further transcriptomic sequencing	814:846	Further transcriptomic sequencing identified Wnt signaling a major regulatory node downstream of Synbindin.
30561033	3	47	theme	Synbindin	317:325	arg1	upregulation					327:338	Synbindin upregulation	317:338	Synbindin upregulation in human CRCs	317:352	Synbindin upregulation in human CRCs was associated with poor patient prognosis.
30561033	4	48	theme	disturbed	454:462	arg1	microbiota					468:477	the disturbed gut microbiota	450:477	the disturbed gut microbiota	450:477	Intestine-specific disruption of Synbindin balanced the disturbed gut microbiota and protected mice against tumor formation in the colitis-associated cancer (CAC) model.
30561033	8	49	theme	-based	1023:1028	arg1	assembly					1042:1049	Disheveled-3 (DVL3)-based signalosome assembly	1004:1049	Disheveled-3 (DVL3)-based signalosome assembly	1004:1049	Combined molecular and cellular characterizations revealed that Synbindin confers Disheveled-3 (DVL3)-based signalosome assembly and acts as a modular scaffold for DVL3 and Axin2 complex, orchestrating the intensity of Wnt signaling.
30561033	9	50	with	protein	1336:1342	arg1	roles					1362:1366	multifaceted roles	1349:1366	multifaceted roles	1349:1366	These findings identify a critical role of Synbindin in gut microbiome composition and Wnt signaling activation in colorectal carcinogenesis, and highlight Synbindin as an adaptor protein with multifaceted roles.
30561033	7	51	theme	transcriptomic	822:835	arg1	sequencing					837:846	Further transcriptomic sequencing	814:846	Further transcriptomic sequencing	814:846	Further transcriptomic sequencing identified Wnt signaling a major regulatory node downstream of Synbindin.
30561033	6	52	theme	increased	645:653	arg1	cells					662:666	increased goblet cells	645:666	increased goblet cells	645:666	In host, increased goblet cells and mucin2 expression, together with increased intestinal epithelial cells (IECs) apoptosis and decreased epithelial proliferation were observed.
30561033	9	53	theme	multifaceted	1349:1360	arg1	roles					1362:1366	multifaceted roles	1349:1366	multifaceted roles	1349:1366	These findings identify a critical role of Synbindin in gut microbiome composition and Wnt signaling activation in colorectal carcinogenesis, and highlight Synbindin as an adaptor protein with multifaceted roles.
30561033	9	54	theme	microbiome	1216:1225	arg1	composition					1227:1237	gut microbiome composition	1212:1237	gut microbiome composition	1212:1237	These findings identify a critical role of Synbindin in gut microbiome composition and Wnt signaling activation in colorectal carcinogenesis, and highlight Synbindin as an adaptor protein with multifaceted roles.
30561033	9	55	theme	Synbindin	1199:1207	arg1	role					1191:1194	a critical role	1180:1194	a critical role of Synbindin in gut microbiome composition and Wnt signaling activation in colorectal carcinogenesis	1180:1295	These findings identify a critical role of Synbindin in gut microbiome composition and Wnt signaling activation in colorectal carcinogenesis, and highlight Synbindin as an adaptor protein with multifaceted roles.
30561033	6	56	theme	epithelial	774:783	arg1	proliferation					785:797	decreased epithelial proliferation	764:797	decreased epithelial proliferation	764:797	In host, increased goblet cells and mucin2 expression, together with increased intestinal epithelial cells (IECs) apoptosis and decreased epithelial proliferation were observed.
30561033	9	57	theme	critical	1182:1189	arg1	role					1191:1194	a critical role	1180:1194	a critical role of Synbindin in gut microbiome composition and Wnt signaling activation in colorectal carcinogenesis	1180:1295	These findings identify a critical role of Synbindin in gut microbiome composition and Wnt signaling activation in colorectal carcinogenesis, and highlight Synbindin as an adaptor protein with multifaceted roles.
30561033	3	58	theme	human	343:347	arg1	CRCs					349:352	human CRCs	343:352	human CRCs	343:352	Synbindin upregulation in human CRCs was associated with poor patient prognosis.
30561033	4	59	theme	gut	464:466	arg1	microbiota					468:477	the disturbed gut microbiota	450:477	the disturbed gut microbiota	450:477	Intestine-specific disruption of Synbindin balanced the disturbed gut microbiota and protected mice against tumor formation in the colitis-associated cancer (CAC) model.
30561033	6	60	theme	decreased	764:772	arg1	proliferation					785:797	decreased epithelial proliferation	764:797	decreased epithelial proliferation	764:797	In host, increased goblet cells and mucin2 expression, together with increased intestinal epithelial cells (IECs) apoptosis and decreased epithelial proliferation were observed.
30561033	6	61	theme	mucin2	672:677	arg1	expression					679:688	mucin2 expression	672:688	mucin2 expression	672:688	In host, increased goblet cells and mucin2 expression, together with increased intestinal epithelial cells (IECs) apoptosis and decreased epithelial proliferation were observed.
30561033	4	62	theme	cancer	548:553	arg1	model					561:565	the colitis-associated cancer (CAC) model	525:565	the colitis-associated cancer (CAC) model	525:565	Intestine-specific disruption of Synbindin balanced the disturbed gut microbiota and protected mice against tumor formation in the colitis-associated cancer (CAC) model.
30561033	2	63	theme	CRC	244:246	arg1	oncogenesis					248:258	CRC oncogenesis	244:258	CRC oncogenesis	244:258	Here we show Synbindin promoted CRC oncogenesis by activating Wnt signaling and altering gut microbiome.
30561033	9	64	theme	Wnt	1243:1245	arg1	activation					1257:1266	Wnt signaling activation	1243:1266	Wnt signaling activation	1243:1266	These findings identify a critical role of Synbindin in gut microbiome composition and Wnt signaling activation in colorectal carcinogenesis, and highlight Synbindin as an adaptor protein with multifaceted roles.
29652253	5	0	theme	building	609:616	arg1	tools					618:622	these enhanced building tools	594:622	these enhanced building tools	594:622	It is hoped that these enhanced building tools will help to reduce building errors of N-linked glycans and improve our knowledge of the structures of glycoproteins.
29652253	1	1	theme	residue	222:228	arg1	level					230:234	the residue level	218:234	the residue level	218:234	Coot is a graphics application that is used to build or manipulate macromolecular models; its particular forte is manipulation of the model at the residue level.
29652253	5	2	theme	glycoproteins	727:739	arg1	structures					713:722	the structures	709:722	the structures of glycoproteins	709:739	It is hoped that these enhanced building tools will help to reduce building errors of N-linked glycans and improve our knowledge of the structures of glycoproteins.
29652253	1	3	theme	macromolecular	142:155	arg1	models					157:162	macromolecular models	142:162	macromolecular models	142:162	Coot is a graphics application that is used to build or manipulate macromolecular models; its particular forte is manipulation of the model at the residue level.
29652253	2	4	theme	Coot	265:268	arg1	tools					256:260	The model-building tools	237:260	The model-building tools of Coot	237:268	The model-building tools of Coot have been combined and extended to assist or automate the building of N-linked glycans.
29652253	4	5	theme	additional	554:563	arg1	restraints					565:574	modified and additional restraints	541:574	modified and additional restraints	541:574	The subsequent model is refined by real-space refinement, which is stabilized with modified and additional restraints.
29652253	5	6	gly	glycoproteins	727:739	arg1	glycoproteins					727:739	glycoproteins	727:739	glycoproteins	727:739	It is hoped that these enhanced building tools will help to reduce building errors of N-linked glycans and improve our knowledge of the structures of glycoproteins.
29652253	4	7	theme	modified	541:548	arg1	restraints					565:574	modified and additional restraints	541:574	modified and additional restraints	541:574	The subsequent model is refined by real-space refinement, which is stabilized with modified and additional restraints.
29652253	2	8	theme	model-building	241:254	arg1	tools					256:260	The model-building tools	237:260	The model-building tools of Coot	237:268	The model-building tools of Coot have been combined and extended to assist or automate the building of N-linked glycans.
29652253	3	9	theme	monosaccharides	396:410	arg1	addition					384:391	the addition	380:391	the addition	380:391	The model is built by the addition of monosaccharides, placed by variation of internal coordinates.
29652253	0	10	link	N-linked	47:54	arg1	glycans					56:62	building N-linked glycans	38:62	Structural analysis of glycoproteins: building N-linked glycans with Coot.	0:73	Structural analysis of glycoproteins: building N-linked glycans with Coot.
29652253	2	11	theme	glycans	349:355	arg1	building					328:335	the building	324:335	the building of N-linked glycans	324:355	The model-building tools of Coot have been combined and extended to assist or automate the building of N-linked glycans.
29652253	0	12	theme	Structural	0:9	arg1	analysis					11:18	Structural analysis	0:18	Structural analysis of glycoproteins: building N-linked glycans with Coot.	0:73	Structural analysis of glycoproteins: building N-linked glycans with Coot.
29652253	5	13	theme	enhanced	600:607	arg1	tools					618:622	these enhanced building tools	594:622	these enhanced building tools	594:622	It is hoped that these enhanced building tools will help to reduce building errors of N-linked glycans and improve our knowledge of the structures of glycoproteins.
29652253	1	14	used	used	114:117	arg2	application					94:104	a graphics application	83:104	a graphics application that is used to build or manipulate macromolecular models	83:162	Coot is a graphics application that is used to build or manipulate macromolecular models; its particular forte is manipulation of the model at the residue level.
29652253	1	14	used	used	114:117	arg2	Coot					75:78	Coot	75:78	Coot	75:78	Coot is a graphics application that is used to build or manipulate macromolecular models; its particular forte is manipulation of the model at the residue level.
29652253	2	15	theme	N-linked	340:347	arg1	glycans					349:355	N-linked glycans	340:355	N-linked glycans	340:355	The model-building tools of Coot have been combined and extended to assist or automate the building of N-linked glycans.
29652253	0	16	theme	glycoproteins	23:35	arg1	analysis					11:18	Structural analysis	0:18	Structural analysis of glycoproteins: building N-linked glycans with Coot.	0:73	Structural analysis of glycoproteins: building N-linked glycans with Coot.
29652253	1	17	theme	graphics	85:92	arg1	Coot					75:78	Coot	75:78	Coot	75:78	Coot is a graphics application that is used to build or manipulate macromolecular models; its particular forte is manipulation of the model at the residue level.
29652253	1	17	theme	graphics	85:92	arg1	application					94:104	a graphics application	83:104	a graphics application that is used to build or manipulate macromolecular models	83:162	Coot is a graphics application that is used to build or manipulate macromolecular models; its particular forte is manipulation of the model at the residue level.
29652253	5	18	theme	N-linked	663:670	arg1	glycans					672:678	N-linked glycans	663:678	N-linked glycans	663:678	It is hoped that these enhanced building tools will help to reduce building errors of N-linked glycans and improve our knowledge of the structures of glycoproteins.
29652253	5	19	link	N-linked	663:670	arg1	glycans					672:678	N-linked glycans	663:678	N-linked glycans	663:678	It is hoped that these enhanced building tools will help to reduce building errors of N-linked glycans and improve our knowledge of the structures of glycoproteins.
29652253	0	20	theme	building	38:45	arg1	glycans					56:62	building N-linked glycans	38:62	Structural analysis of glycoproteins: building N-linked glycans with Coot.	0:73	Structural analysis of glycoproteins: building N-linked glycans with Coot.
29652253	5	21	theme	glycans	672:678	arg1	errors					653:658	building errors	644:658	building errors of N-linked glycans	644:678	It is hoped that these enhanced building tools will help to reduce building errors of N-linked glycans and improve our knowledge of the structures of glycoproteins.
29652253	2	22	link	N-linked	340:347	arg1	glycans					349:355	N-linked glycans	340:355	N-linked glycans	340:355	The model-building tools of Coot have been combined and extended to assist or automate the building of N-linked glycans.
29652253	1	23	from	level	230:234	arg1	manipulation					189:200	manipulation	189:200	manipulation of the model at the residue level	189:234	Coot is a graphics application that is used to build or manipulate macromolecular models; its particular forte is manipulation of the model at the residue level.
29652253	3	24	theme	coordinates	445:455	arg1	variation					423:431	variation	423:431	variation of internal coordinates	423:455	The model is built by the addition of monosaccharides, placed by variation of internal coordinates.
29652253	0	25	theme	N-linked	47:54	arg1	glycans					56:62	building N-linked glycans	38:62	Structural analysis of glycoproteins: building N-linked glycans with Coot.	0:73	Structural analysis of glycoproteins: building N-linked glycans with Coot.
29652253	0	26	gly	glycoproteins	23:35	arg1	glycoproteins					23:35	glycoproteins	23:35	glycoproteins	23:35	Structural analysis of glycoproteins: building N-linked glycans with Coot.
29652253	4	27	theme	real-space	493:502	arg1	refinement					504:513	real-space refinement	493:513	real-space refinement	493:513	The subsequent model is refined by real-space refinement, which is stabilized with modified and additional restraints.
29652253	5	28	theme	building	644:651	arg1	errors					653:658	building errors	644:658	building errors of N-linked glycans	644:678	It is hoped that these enhanced building tools will help to reduce building errors of N-linked glycans and improve our knowledge of the structures of glycoproteins.
29652253	0	29	with	glycans	56:62	arg1	Coot					69:72	Coot	69:72	Coot	69:72	Structural analysis of glycoproteins: building N-linked glycans with Coot.
29652253	3	30	theme	internal	436:443	arg1	coordinates					445:455	internal coordinates	436:455	internal coordinates	436:455	The model is built by the addition of monosaccharides, placed by variation of internal coordinates.
29652253	1	31	theme	model	209:213	arg1	manipulation					189:200	manipulation	189:200	manipulation of the model at the residue level	189:234	Coot is a graphics application that is used to build or manipulate macromolecular models; its particular forte is manipulation of the model at the residue level.
29652253	0	32	dep	analysis	11:18	arg1	glycans					56:62	building N-linked glycans	38:62	Structural analysis of glycoproteins: building N-linked glycans with Coot.	0:73	Structural analysis of glycoproteins: building N-linked glycans with Coot.
29652253	5	33	theme	structures	713:722	arg1	knowledge					696:704	our knowledge	692:704	our knowledge of the structures of glycoproteins	692:739	It is hoped that these enhanced building tools will help to reduce building errors of N-linked glycans and improve our knowledge of the structures of glycoproteins.
29652253	4	34	theme	subsequent	462:471	arg1	model					473:477	The subsequent model	458:477	The subsequent model	458:477	The subsequent model is refined by real-space refinement, which is stabilized with modified and additional restraints.
35542817	0	0	theme	Nitraria	80:87	arg1	Bobr					100:103	Nitraria tangutorum Bobr	80:103	Nitraria tangutorum Bobr	80:103	Purification, structure and anti-oxidation of polysaccharides from the fruit of Nitraria tangutorum Bobr.
35542817	3	1	theme	optimal	293:299	arg1	follows					331:337	follows	331:337	follows	331:337	The optimal conditions were determined as follows: extraction time 7 h, extraction temperature 60 °C, ratio of water to raw material 15 : 1, and with these conditions, the yield was 14.01 ± 0.11%.
35542817	3	1	theme	optimal	293:299	arg1	conditions					301:310	The optimal conditions	289:310	The optimal conditions	289:310	The optimal conditions were determined as follows: extraction time 7 h, extraction temperature 60 °C, ratio of water to raw material 15 : 1, and with these conditions, the yield was 14.01 ± 0.11%.
35542817	5	2	theme	NTWP-II	682:688	arg1	composition					667:677	monosaccharide composition	652:677	monosaccharide composition of NTWP-II	652:688	The results of GC-MS and SEC-LLS analysis suggested that monosaccharide composition of NTWP-II was composed of Rha, Ara, Man, Glc and Gal with the molar ratio of 1.14 : 2.5 : 3.00 : 2.69 : 5.28 and M w, M w/M n and R z 2.29 × 105, 1.32, 15.22.
35542817	0	3	theme	Bobr	100:103	arg1	fruit					71:75	the fruit	67:75	the fruit of Nitraria tangutorum Bobr	67:103	Purification, structure and anti-oxidation of polysaccharides from the fruit of Nitraria tangutorum Bobr.
35542817	4	4	theme	Sephadex	537:544	arg1	column					552:557	Sephadex G-200 column	537:557	Sephadex G-200 column	537:557	After purification using DEAE-cellulose column and Sephadex G-200 column, NTWP-II was successfully obtained.
35542817	3	5	dep	h	358:358	arg1	 1					426:427	 1	426:427	 1	426:427	The optimal conditions were determined as follows: extraction time 7 h, extraction temperature 60 °C, ratio of water to raw material 15 : 1, and with these conditions, the yield was 14.01 ± 0.11%.
35542817	5	6	with	Glc	721:723	arg1	ratio					748:752	the molar ratio	738:752	the molar ratio of 1.14 	738:761	The results of GC-MS and SEC-LLS analysis suggested that monosaccharide composition of NTWP-II was composed of Rha, Ara, Man, Glc and Gal with the molar ratio of 1.14 : 2.5 : 3.00 : 2.69 : 5.28 and M w, M w/M n and R z 2.29 × 105, 1.32, 15.22.
35542817	5	6	with	Glc	721:723	arg1	15.22					832:836	15.22	832:836	15.22	832:836	The results of GC-MS and SEC-LLS analysis suggested that monosaccharide composition of NTWP-II was composed of Rha, Ara, Man, Glc and Gal with the molar ratio of 1.14 : 2.5 : 3.00 : 2.69 : 5.28 and M w, M w/M n and R z 2.29 × 105, 1.32, 15.22.
35542817	5	6	with	Glc	721:723	arg1	×					819:819	M w/M n and R z 2.29 × 105	798:823	×	819:819	The results of GC-MS and SEC-LLS analysis suggested that monosaccharide composition of NTWP-II was composed of Rha, Ara, Man, Glc and Gal with the molar ratio of 1.14 : 2.5 : 3.00 : 2.69 : 5.28 and M w, M w/M n and R z 2.29 × 105, 1.32, 15.22.
35542817	5	6	with	Glc	721:723	arg1	n					804:804	M w/M n and R z 2.29 × 105	798:823	n	804:804	The results of GC-MS and SEC-LLS analysis suggested that monosaccharide composition of NTWP-II was composed of Rha, Ara, Man, Glc and Gal with the molar ratio of 1.14 : 2.5 : 3.00 : 2.69 : 5.28 and M w, M w/M n and R z 2.29 × 105, 1.32, 15.22.
35542817	4	7	theme	DEAE-cellulose	511:524	arg1	column					526:531	DEAE-cellulose column	511:531	DEAE-cellulose column	511:531	After purification using DEAE-cellulose column and Sephadex G-200 column, NTWP-II was successfully obtained.
35542817	0	8	theme	tangutorum	89:98	arg1	Bobr					100:103	Nitraria tangutorum Bobr	80:103	Nitraria tangutorum Bobr	80:103	Purification, structure and anti-oxidation of polysaccharides from the fruit of Nitraria tangutorum Bobr.
35542817	5	9	with	Man	716:718	arg1	ratio					748:752	the molar ratio	738:752	the molar ratio of 1.14 	738:761	The results of GC-MS and SEC-LLS analysis suggested that monosaccharide composition of NTWP-II was composed of Rha, Ara, Man, Glc and Gal with the molar ratio of 1.14 : 2.5 : 3.00 : 2.69 : 5.28 and M w, M w/M n and R z 2.29 × 105, 1.32, 15.22.
35542817	5	9	with	Man	716:718	arg1	15.22					832:836	15.22	832:836	15.22	832:836	The results of GC-MS and SEC-LLS analysis suggested that monosaccharide composition of NTWP-II was composed of Rha, Ara, Man, Glc and Gal with the molar ratio of 1.14 : 2.5 : 3.00 : 2.69 : 5.28 and M w, M w/M n and R z 2.29 × 105, 1.32, 15.22.
35542817	5	9	with	Man	716:718	arg1	×					819:819	M w/M n and R z 2.29 × 105	798:823	×	819:819	The results of GC-MS and SEC-LLS analysis suggested that monosaccharide composition of NTWP-II was composed of Rha, Ara, Man, Glc and Gal with the molar ratio of 1.14 : 2.5 : 3.00 : 2.69 : 5.28 and M w, M w/M n and R z 2.29 × 105, 1.32, 15.22.
35542817	5	9	with	Man	716:718	arg1	n					804:804	M w/M n and R z 2.29 × 105	798:823	n	804:804	The results of GC-MS and SEC-LLS analysis suggested that monosaccharide composition of NTWP-II was composed of Rha, Ara, Man, Glc and Gal with the molar ratio of 1.14 : 2.5 : 3.00 : 2.69 : 5.28 and M w, M w/M n and R z 2.29 × 105, 1.32, 15.22.
35542817	3	10	theme	14.01	471:475	arg1	%					483:483	14.01 ± 0.11%	471:483	14.01 ± 0.11%	471:483	The optimal conditions were determined as follows: extraction time 7 h, extraction temperature 60 °C, ratio of water to raw material 15 : 1, and with these conditions, the yield was 14.01 ± 0.11%.
35542817	3	10	theme	14.01	471:475	arg1	yield					461:465	the yield	457:465	the yield	457:465	The optimal conditions were determined as follows: extraction time 7 h, extraction temperature 60 °C, ratio of water to raw material 15 : 1, and with these conditions, the yield was 14.01 ± 0.11%.
35542817	7	11	theme	NTWP-II	970:976	arg1	unit					962:965	the repeating unit	948:965	the repeating unit of NTWP-II	948:976	Based on these analyses, the structure of the repeating unit of NTWP-II was established.
35542817	5	12	theme	R	810:810	arg1	ratio					748:752	the molar ratio	738:752	the molar ratio of 1.14 	738:761	The results of GC-MS and SEC-LLS analysis suggested that monosaccharide composition of NTWP-II was composed of Rha, Ara, Man, Glc and Gal with the molar ratio of 1.14 : 2.5 : 3.00 : 2.69 : 5.28 and M w, M w/M n and R z 2.29 × 105, 1.32, 15.22.
35542817	5	12	theme	R	810:810	arg1	×					819:819	M w/M n and R z 2.29 × 105	798:823	×	819:819	The results of GC-MS and SEC-LLS analysis suggested that monosaccharide composition of NTWP-II was composed of Rha, Ara, Man, Glc and Gal with the molar ratio of 1.14 : 2.5 : 3.00 : 2.69 : 5.28 and M w, M w/M n and R z 2.29 × 105, 1.32, 15.22.
35542817	0	13	theme	polysaccharides	46:60	arg1	structure					14:22	structure	14:22	structure	14:22	Purification, structure and anti-oxidation of polysaccharides from the fruit of Nitraria tangutorum Bobr.
35542817	0	13	theme	polysaccharides	46:60	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification, structure and anti-oxidation of polysaccharides from the fruit of Nitraria tangutorum Bobr.
35542817	0	13	theme	polysaccharides	46:60	arg1	anti-oxidation					28:41	anti-oxidation	28:41	anti-oxidation	28:41	Purification, structure and anti-oxidation of polysaccharides from the fruit of Nitraria tangutorum Bobr.
35542817	2	14	theme	extraction	244:253	arg1	conditions					255:264	extraction conditions	244:264	extraction conditions	244:264	(NTWP) using a hot water extraction method and extraction conditions were optimized by RSM.
35542817	3	15	theme	water	400:404	arg1	h					358:358	extraction time 7 h	340:358	extraction time 7 h	340:358	The optimal conditions were determined as follows: extraction time 7 h, extraction temperature 60 °C, ratio of water to raw material 15 : 1, and with these conditions, the yield was 14.01 ± 0.11%.
35542817	3	15	theme	water	400:404	arg1	ratio					391:395	ratio	391:395	ratio of water to raw material 15 	391:424	The optimal conditions were determined as follows: extraction time 7 h, extraction temperature 60 °C, ratio of water to raw material 15 : 1, and with these conditions, the yield was 14.01 ± 0.11%.
35542817	7	16	theme	unit	962:965	arg1	structure					935:943	the structure	931:943	the structure of the repeating unit of NTWP-II	931:976	Based on these analyses, the structure of the repeating unit of NTWP-II was established.
35542817	5	17	dep	ratio	748:752	arg1	 5.28					783:787	 5.28	783:787	 5.28	783:787	The results of GC-MS and SEC-LLS analysis suggested that monosaccharide composition of NTWP-II was composed of Rha, Ara, Man, Glc and Gal with the molar ratio of 1.14 : 2.5 : 3.00 : 2.69 : 5.28 and M w, M w/M n and R z 2.29 × 105, 1.32, 15.22.
35542817	5	17	dep	ratio	748:752	arg1	w					795:795	M w	793:795	M w	793:795	The results of GC-MS and SEC-LLS analysis suggested that monosaccharide composition of NTWP-II was composed of Rha, Ara, Man, Glc and Gal with the molar ratio of 1.14 : 2.5 : 3.00 : 2.69 : 5.28 and M w, M w/M n and R z 2.29 × 105, 1.32, 15.22.
35542817	5	17	dep	ratio	748:752	arg1	 2.69 					776:781	 2.69 	776:781	 2.69 	776:781	The results of GC-MS and SEC-LLS analysis suggested that monosaccharide composition of NTWP-II was composed of Rha, Ara, Man, Glc and Gal with the molar ratio of 1.14 : 2.5 : 3.00 : 2.69 : 5.28 and M w, M w/M n and R z 2.29 × 105, 1.32, 15.22.
35542817	5	17	dep	ratio	748:752	arg1	 3.00 					769:774	 3.00 	769:774	 3.00 	769:774	The results of GC-MS and SEC-LLS analysis suggested that monosaccharide composition of NTWP-II was composed of Rha, Ara, Man, Glc and Gal with the molar ratio of 1.14 : 2.5 : 3.00 : 2.69 : 5.28 and M w, M w/M n and R z 2.29 × 105, 1.32, 15.22.
35542817	5	17	dep	ratio	748:752	arg1	 2.5 					763:767	 2.5 	763:767	 2.5 	763:767	The results of GC-MS and SEC-LLS analysis suggested that monosaccharide composition of NTWP-II was composed of Rha, Ara, Man, Glc and Gal with the molar ratio of 1.14 : 2.5 : 3.00 : 2.69 : 5.28 and M w, M w/M n and R z 2.29 × 105, 1.32, 15.22.
35542817	1	18	theme	Nitraria	171:178	arg1	Bobr					191:194	Nitraria tangutorum Bobr	171:194	Nitraria tangutorum Bobr	171:194	In this paper, polysaccharides were extracted from the fruits of Nitraria tangutorum Bobr.
35542817	5	19	with	Gal	729:731	arg1	ratio					748:752	the molar ratio	738:752	the molar ratio of 1.14 	738:761	The results of GC-MS and SEC-LLS analysis suggested that monosaccharide composition of NTWP-II was composed of Rha, Ara, Man, Glc and Gal with the molar ratio of 1.14 : 2.5 : 3.00 : 2.69 : 5.28 and M w, M w/M n and R z 2.29 × 105, 1.32, 15.22.
35542817	5	19	with	Gal	729:731	arg1	15.22					832:836	15.22	832:836	15.22	832:836	The results of GC-MS and SEC-LLS analysis suggested that monosaccharide composition of NTWP-II was composed of Rha, Ara, Man, Glc and Gal with the molar ratio of 1.14 : 2.5 : 3.00 : 2.69 : 5.28 and M w, M w/M n and R z 2.29 × 105, 1.32, 15.22.
35542817	5	19	with	Gal	729:731	arg1	×					819:819	M w/M n and R z 2.29 × 105	798:823	×	819:819	The results of GC-MS and SEC-LLS analysis suggested that monosaccharide composition of NTWP-II was composed of Rha, Ara, Man, Glc and Gal with the molar ratio of 1.14 : 2.5 : 3.00 : 2.69 : 5.28 and M w, M w/M n and R z 2.29 × 105, 1.32, 15.22.
35542817	5	19	with	Gal	729:731	arg1	n					804:804	M w/M n and R z 2.29 × 105	798:823	n	804:804	The results of GC-MS and SEC-LLS analysis suggested that monosaccharide composition of NTWP-II was composed of Rha, Ara, Man, Glc and Gal with the molar ratio of 1.14 : 2.5 : 3.00 : 2.69 : 5.28 and M w, M w/M n and R z 2.29 × 105, 1.32, 15.22.
35542817	1	20	theme	tangutorum	180:189	arg1	Bobr					191:194	Nitraria tangutorum Bobr	171:194	Nitraria tangutorum Bobr	171:194	In this paper, polysaccharides were extracted from the fruits of Nitraria tangutorum Bobr.
35542817	3	21	theme	time	351:354	arg1	h					358:358	extraction time 7 h	340:358	extraction time 7 h	340:358	The optimal conditions were determined as follows: extraction time 7 h, extraction temperature 60 °C, ratio of water to raw material 15 : 1, and with these conditions, the yield was 14.01 ± 0.11%.
35542817	3	21	theme	time	351:354	arg1	ratio					391:395	ratio	391:395	ratio of water to raw material 15 	391:424	The optimal conditions were determined as follows: extraction time 7 h, extraction temperature 60 °C, ratio of water to raw material 15 : 1, and with these conditions, the yield was 14.01 ± 0.11%.
35542817	3	21	theme	time	351:354	arg1	°C					387:388	extraction temperature 60 °C	361:388	extraction temperature 60 °C	361:388	The optimal conditions were determined as follows: extraction time 7 h, extraction temperature 60 °C, ratio of water to raw material 15 : 1, and with these conditions, the yield was 14.01 ± 0.11%.
35542817	5	22	theme	SEC-LLS	620:626	arg1	analysis					628:635	GC-MS and SEC-LLS analysis	610:635	analysis	628:635	The results of GC-MS and SEC-LLS analysis suggested that monosaccharide composition of NTWP-II was composed of Rha, Ara, Man, Glc and Gal with the molar ratio of 1.14 : 2.5 : 3.00 : 2.69 : 5.28 and M w, M w/M n and R z 2.29 × 105, 1.32, 15.22.
35542817	3	23	theme	±	477:477	arg1	%					483:483	14.01 ± 0.11%	471:483	14.01 ± 0.11%	471:483	The optimal conditions were determined as follows: extraction time 7 h, extraction temperature 60 °C, ratio of water to raw material 15 : 1, and with these conditions, the yield was 14.01 ± 0.11%.
35542817	3	23	theme	±	477:477	arg1	yield					461:465	the yield	457:465	the yield	457:465	The optimal conditions were determined as follows: extraction time 7 h, extraction temperature 60 °C, ratio of water to raw material 15 : 1, and with these conditions, the yield was 14.01 ± 0.11%.
35542817	5	24	theme	1.32	826:829	arg1	ratio					748:752	the molar ratio	738:752	the molar ratio of 1.14 	738:761	The results of GC-MS and SEC-LLS analysis suggested that monosaccharide composition of NTWP-II was composed of Rha, Ara, Man, Glc and Gal with the molar ratio of 1.14 : 2.5 : 3.00 : 2.69 : 5.28 and M w, M w/M n and R z 2.29 × 105, 1.32, 15.22.
35542817	5	24	theme	1.32	826:829	arg1	15.22					832:836	15.22	832:836	15.22	832:836	The results of GC-MS and SEC-LLS analysis suggested that monosaccharide composition of NTWP-II was composed of Rha, Ara, Man, Glc and Gal with the molar ratio of 1.14 : 2.5 : 3.00 : 2.69 : 5.28 and M w, M w/M n and R z 2.29 × 105, 1.32, 15.22.
35542817	5	24	theme	1.32	826:829	arg1	×					819:819	M w/M n and R z 2.29 × 105	798:823	×	819:819	The results of GC-MS and SEC-LLS analysis suggested that monosaccharide composition of NTWP-II was composed of Rha, Ara, Man, Glc and Gal with the molar ratio of 1.14 : 2.5 : 3.00 : 2.69 : 5.28 and M w, M w/M n and R z 2.29 × 105, 1.32, 15.22.
35542817	5	24	theme	1.32	826:829	arg1	n					804:804	M w/M n and R z 2.29 × 105	798:823	n	804:804	The results of GC-MS and SEC-LLS analysis suggested that monosaccharide composition of NTWP-II was composed of Rha, Ara, Man, Glc and Gal with the molar ratio of 1.14 : 2.5 : 3.00 : 2.69 : 5.28 and M w, M w/M n and R z 2.29 × 105, 1.32, 15.22.
35542817	2	25	dep	using	204:208	arg1	NTWP					198:201	NTWP	198:201	NTWP	198:201	(NTWP) using a hot water extraction method and extraction conditions were optimized by RSM.
35542817	7	26	theme	repeating	952:960	arg1	unit					962:965	the repeating unit	948:965	the repeating unit of NTWP-II	948:976	Based on these analyses, the structure of the repeating unit of NTWP-II was established.
35542817	5	27	theme	analysis	628:635	arg1	results					599:605	The results	595:605	The results of GC-MS and SEC-LLS analysis	595:635	The results of GC-MS and SEC-LLS analysis suggested that monosaccharide composition of NTWP-II was composed of Rha, Ara, Man, Glc and Gal with the molar ratio of 1.14 : 2.5 : 3.00 : 2.69 : 5.28 and M w, M w/M n and R z 2.29 × 105, 1.32, 15.22.
35542817	5	28	theme	w/M	800:802	arg1	ratio					748:752	the molar ratio	738:752	the molar ratio of 1.14 	738:761	The results of GC-MS and SEC-LLS analysis suggested that monosaccharide composition of NTWP-II was composed of Rha, Ara, Man, Glc and Gal with the molar ratio of 1.14 : 2.5 : 3.00 : 2.69 : 5.28 and M w, M w/M n and R z 2.29 × 105, 1.32, 15.22.
35542817	5	28	theme	w/M	800:802	arg1	n					804:804	M w/M n and R z 2.29 × 105	798:823	n	804:804	The results of GC-MS and SEC-LLS analysis suggested that monosaccharide composition of NTWP-II was composed of Rha, Ara, Man, Glc and Gal with the molar ratio of 1.14 : 2.5 : 3.00 : 2.69 : 5.28 and M w, M w/M n and R z 2.29 × 105, 1.32, 15.22.
35542817	6	29	theme	detailed	843:850	arg1	structure					852:860	The detailed structure	839:860	The detailed structure of NTWP-II	839:871	The detailed structure of NTWP-II was characterized by FT-IR, NMR.
35542817	2	30	theme	hot	212:214	arg1	method					233:238	a hot water extraction method	210:238	a hot water extraction method	210:238	(NTWP) using a hot water extraction method and extraction conditions were optimized by RSM.
35542817	6	31	theme	NTWP-II	865:871	arg1	structure					852:860	The detailed structure	839:860	The detailed structure of NTWP-II	839:871	The detailed structure of NTWP-II was characterized by FT-IR, NMR.
35542817	5	32	theme	molar	742:746	arg1	ratio					748:752	the molar ratio	738:752	the molar ratio of 1.14 	738:761	The results of GC-MS and SEC-LLS analysis suggested that monosaccharide composition of NTWP-II was composed of Rha, Ara, Man, Glc and Gal with the molar ratio of 1.14 : 2.5 : 3.00 : 2.69 : 5.28 and M w, M w/M n and R z 2.29 × 105, 1.32, 15.22.
35542817	5	32	theme	molar	742:746	arg1	15.22					832:836	15.22	832:836	15.22	832:836	The results of GC-MS and SEC-LLS analysis suggested that monosaccharide composition of NTWP-II was composed of Rha, Ara, Man, Glc and Gal with the molar ratio of 1.14 : 2.5 : 3.00 : 2.69 : 5.28 and M w, M w/M n and R z 2.29 × 105, 1.32, 15.22.
35542817	5	32	theme	molar	742:746	arg1	×					819:819	M w/M n and R z 2.29 × 105	798:823	×	819:819	The results of GC-MS and SEC-LLS analysis suggested that monosaccharide composition of NTWP-II was composed of Rha, Ara, Man, Glc and Gal with the molar ratio of 1.14 : 2.5 : 3.00 : 2.69 : 5.28 and M w, M w/M n and R z 2.29 × 105, 1.32, 15.22.
35542817	5	32	theme	molar	742:746	arg1	n					804:804	M w/M n and R z 2.29 × 105	798:823	n	804:804	The results of GC-MS and SEC-LLS analysis suggested that monosaccharide composition of NTWP-II was composed of Rha, Ara, Man, Glc and Gal with the molar ratio of 1.14 : 2.5 : 3.00 : 2.69 : 5.28 and M w, M w/M n and R z 2.29 × 105, 1.32, 15.22.
35542817	0	33	from	fruit	71:75	arg1	structure					14:22	structure	14:22	structure	14:22	Purification, structure and anti-oxidation of polysaccharides from the fruit of Nitraria tangutorum Bobr.
35542817	0	33	from	fruit	71:75	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification, structure and anti-oxidation of polysaccharides from the fruit of Nitraria tangutorum Bobr.
35542817	0	33	from	fruit	71:75	arg1	anti-oxidation					28:41	anti-oxidation	28:41	anti-oxidation	28:41	Purification, structure and anti-oxidation of polysaccharides from the fruit of Nitraria tangutorum Bobr.
35542817	3	34	with	%	483:483	arg1	conditions					445:454	these conditions	439:454	these conditions	439:454	The optimal conditions were determined as follows: extraction time 7 h, extraction temperature 60 °C, ratio of water to raw material 15 : 1, and with these conditions, the yield was 14.01 ± 0.11%.
35542817	3	35	theme	raw	409:411	arg1	material					413:420	raw material 15 	409:424	raw material 15 	409:424	The optimal conditions were determined as follows: extraction time 7 h, extraction temperature 60 °C, ratio of water to raw material 15 : 1, and with these conditions, the yield was 14.01 ± 0.11%.
35542817	3	36	theme	extraction	361:370	arg1	h					358:358	extraction time 7 h	340:358	extraction time 7 h	340:358	The optimal conditions were determined as follows: extraction time 7 h, extraction temperature 60 °C, ratio of water to raw material 15 : 1, and with these conditions, the yield was 14.01 ± 0.11%.
35542817	3	36	theme	extraction	361:370	arg1	°C					387:388	extraction temperature 60 °C	361:388	extraction temperature 60 °C	361:388	The optimal conditions were determined as follows: extraction time 7 h, extraction temperature 60 °C, ratio of water to raw material 15 : 1, and with these conditions, the yield was 14.01 ± 0.11%.
35542817	5	37	theme	monosaccharide	652:665	arg1	composition					667:677	monosaccharide composition	652:677	monosaccharide composition of NTWP-II	652:688	The results of GC-MS and SEC-LLS analysis suggested that monosaccharide composition of NTWP-II was composed of Rha, Ara, Man, Glc and Gal with the molar ratio of 1.14 : 2.5 : 3.00 : 2.69 : 5.28 and M w, M w/M n and R z 2.29 × 105, 1.32, 15.22.
35542817	3	38	theme	temperature	372:382	arg1	h					358:358	extraction time 7 h	340:358	extraction time 7 h	340:358	The optimal conditions were determined as follows: extraction time 7 h, extraction temperature 60 °C, ratio of water to raw material 15 : 1, and with these conditions, the yield was 14.01 ± 0.11%.
35542817	3	38	theme	temperature	372:382	arg1	°C					387:388	extraction temperature 60 °C	361:388	extraction temperature 60 °C	361:388	The optimal conditions were determined as follows: extraction time 7 h, extraction temperature 60 °C, ratio of water to raw material 15 : 1, and with these conditions, the yield was 14.01 ± 0.11%.
35542817	3	39	dep	follows	331:337	arg1	h					358:358	extraction time 7 h	340:358	extraction time 7 h	340:358	The optimal conditions were determined as follows: extraction time 7 h, extraction temperature 60 °C, ratio of water to raw material 15 : 1, and with these conditions, the yield was 14.01 ± 0.11%.
35542817	3	39	dep	follows	331:337	arg1	ratio					391:395	ratio	391:395	ratio of water to raw material 15 	391:424	The optimal conditions were determined as follows: extraction time 7 h, extraction temperature 60 °C, ratio of water to raw material 15 : 1, and with these conditions, the yield was 14.01 ± 0.11%.
35542817	3	39	dep	follows	331:337	arg1	°C					387:388	extraction temperature 60 °C	361:388	extraction temperature 60 °C	361:388	The optimal conditions were determined as follows: extraction time 7 h, extraction temperature 60 °C, ratio of water to raw material 15 : 1, and with these conditions, the yield was 14.01 ± 0.11%.
35542817	5	40	theme	GC-MS	610:614	arg1	analysis					628:635	GC-MS and SEC-LLS analysis	610:635	analysis	628:635	The results of GC-MS and SEC-LLS analysis suggested that monosaccharide composition of NTWP-II was composed of Rha, Ara, Man, Glc and Gal with the molar ratio of 1.14 : 2.5 : 3.00 : 2.69 : 5.28 and M w, M w/M n and R z 2.29 × 105, 1.32, 15.22.
35542817	5	41	theme	M	798:798	arg1	ratio					748:752	the molar ratio	738:752	the molar ratio of 1.14 	738:761	The results of GC-MS and SEC-LLS analysis suggested that monosaccharide composition of NTWP-II was composed of Rha, Ara, Man, Glc and Gal with the molar ratio of 1.14 : 2.5 : 3.00 : 2.69 : 5.28 and M w, M w/M n and R z 2.29 × 105, 1.32, 15.22.
35542817	5	41	theme	M	798:798	arg1	n					804:804	M w/M n and R z 2.29 × 105	798:823	n	804:804	The results of GC-MS and SEC-LLS analysis suggested that monosaccharide composition of NTWP-II was composed of Rha, Ara, Man, Glc and Gal with the molar ratio of 1.14 : 2.5 : 3.00 : 2.69 : 5.28 and M w, M w/M n and R z 2.29 × 105, 1.32, 15.22.
35542817	5	42	theme	z	812:812	arg1	ratio					748:752	the molar ratio	738:752	the molar ratio of 1.14 	738:761	The results of GC-MS and SEC-LLS analysis suggested that monosaccharide composition of NTWP-II was composed of Rha, Ara, Man, Glc and Gal with the molar ratio of 1.14 : 2.5 : 3.00 : 2.69 : 5.28 and M w, M w/M n and R z 2.29 × 105, 1.32, 15.22.
35542817	5	42	theme	z	812:812	arg1	×					819:819	M w/M n and R z 2.29 × 105	798:823	×	819:819	The results of GC-MS and SEC-LLS analysis suggested that monosaccharide composition of NTWP-II was composed of Rha, Ara, Man, Glc and Gal with the molar ratio of 1.14 : 2.5 : 3.00 : 2.69 : 5.28 and M w, M w/M n and R z 2.29 × 105, 1.32, 15.22.
35542817	5	43	with	Rha	706:708	arg1	ratio					748:752	the molar ratio	738:752	the molar ratio of 1.14 	738:761	The results of GC-MS and SEC-LLS analysis suggested that monosaccharide composition of NTWP-II was composed of Rha, Ara, Man, Glc and Gal with the molar ratio of 1.14 : 2.5 : 3.00 : 2.69 : 5.28 and M w, M w/M n and R z 2.29 × 105, 1.32, 15.22.
35542817	5	43	with	Rha	706:708	arg1	15.22					832:836	15.22	832:836	15.22	832:836	The results of GC-MS and SEC-LLS analysis suggested that monosaccharide composition of NTWP-II was composed of Rha, Ara, Man, Glc and Gal with the molar ratio of 1.14 : 2.5 : 3.00 : 2.69 : 5.28 and M w, M w/M n and R z 2.29 × 105, 1.32, 15.22.
35542817	5	43	with	Rha	706:708	arg1	×					819:819	M w/M n and R z 2.29 × 105	798:823	×	819:819	The results of GC-MS and SEC-LLS analysis suggested that monosaccharide composition of NTWP-II was composed of Rha, Ara, Man, Glc and Gal with the molar ratio of 1.14 : 2.5 : 3.00 : 2.69 : 5.28 and M w, M w/M n and R z 2.29 × 105, 1.32, 15.22.
35542817	5	43	with	Rha	706:708	arg1	n					804:804	M w/M n and R z 2.29 × 105	798:823	n	804:804	The results of GC-MS and SEC-LLS analysis suggested that monosaccharide composition of NTWP-II was composed of Rha, Ara, Man, Glc and Gal with the molar ratio of 1.14 : 2.5 : 3.00 : 2.69 : 5.28 and M w, M w/M n and R z 2.29 × 105, 1.32, 15.22.
35542817	5	44	theme	M	793:793	arg1	w					795:795	M w	793:795	M w	793:795	The results of GC-MS and SEC-LLS analysis suggested that monosaccharide composition of NTWP-II was composed of Rha, Ara, Man, Glc and Gal with the molar ratio of 1.14 : 2.5 : 3.00 : 2.69 : 5.28 and M w, M w/M n and R z 2.29 × 105, 1.32, 15.22.
35542817	2	45	theme	extraction	222:231	arg1	method					233:238	a hot water extraction method	210:238	a hot water extraction method	210:238	(NTWP) using a hot water extraction method and extraction conditions were optimized by RSM.
35542817	1	46	theme	Bobr	191:194	arg1	fruits					161:166	the fruits	157:166	the fruits of Nitraria tangutorum Bobr	157:194	In this paper, polysaccharides were extracted from the fruits of Nitraria tangutorum Bobr.
35542817	5	47	theme	1.14 	757:761	arg1	ratio					748:752	the molar ratio	738:752	the molar ratio of 1.14 	738:761	The results of GC-MS and SEC-LLS analysis suggested that monosaccharide composition of NTWP-II was composed of Rha, Ara, Man, Glc and Gal with the molar ratio of 1.14 : 2.5 : 3.00 : 2.69 : 5.28 and M w, M w/M n and R z 2.29 × 105, 1.32, 15.22.
35542817	5	47	theme	1.14 	757:761	arg1	15.22					832:836	15.22	832:836	15.22	832:836	The results of GC-MS and SEC-LLS analysis suggested that monosaccharide composition of NTWP-II was composed of Rha, Ara, Man, Glc and Gal with the molar ratio of 1.14 : 2.5 : 3.00 : 2.69 : 5.28 and M w, M w/M n and R z 2.29 × 105, 1.32, 15.22.
35542817	5	47	theme	1.14 	757:761	arg1	×					819:819	M w/M n and R z 2.29 × 105	798:823	×	819:819	The results of GC-MS and SEC-LLS analysis suggested that monosaccharide composition of NTWP-II was composed of Rha, Ara, Man, Glc and Gal with the molar ratio of 1.14 : 2.5 : 3.00 : 2.69 : 5.28 and M w, M w/M n and R z 2.29 × 105, 1.32, 15.22.
35542817	5	47	theme	1.14 	757:761	arg1	n					804:804	M w/M n and R z 2.29 × 105	798:823	n	804:804	The results of GC-MS and SEC-LLS analysis suggested that monosaccharide composition of NTWP-II was composed of Rha, Ara, Man, Glc and Gal with the molar ratio of 1.14 : 2.5 : 3.00 : 2.69 : 5.28 and M w, M w/M n and R z 2.29 × 105, 1.32, 15.22.
35542817	4	48	theme	G-200	546:550	arg1	column					552:557	Sephadex G-200 column	537:557	Sephadex G-200 column	537:557	After purification using DEAE-cellulose column and Sephadex G-200 column, NTWP-II was successfully obtained.
35542817	2	49	theme	water	216:220	arg1	method					233:238	a hot water extraction method	210:238	a hot water extraction method	210:238	(NTWP) using a hot water extraction method and extraction conditions were optimized by RSM.
35542817	3	50	theme	extraction	340:349	arg1	h					358:358	extraction time 7 h	340:358	extraction time 7 h	340:358	The optimal conditions were determined as follows: extraction time 7 h, extraction temperature 60 °C, ratio of water to raw material 15 : 1, and with these conditions, the yield was 14.01 ± 0.11%.
35542817	3	50	theme	extraction	340:349	arg1	ratio					391:395	ratio	391:395	ratio of water to raw material 15 	391:424	The optimal conditions were determined as follows: extraction time 7 h, extraction temperature 60 °C, ratio of water to raw material 15 : 1, and with these conditions, the yield was 14.01 ± 0.11%.
35542817	3	50	theme	extraction	340:349	arg1	°C					387:388	extraction temperature 60 °C	361:388	extraction temperature 60 °C	361:388	The optimal conditions were determined as follows: extraction time 7 h, extraction temperature 60 °C, ratio of water to raw material 15 : 1, and with these conditions, the yield was 14.01 ± 0.11%.
29546983	6	0	link	N-linked	1196:1203	arg1	glycans					1205:1211	43 N-linked glycans	1193:1211	43 N-linked glycans identified after the enrichment of oxidized metal-free carbon	1193:1273	From a real complex sample of a healthy human serum, there were 43 N-linked glycans identified after the enrichment of oxidized metal-free carbon.
29546983	4	1	theme	detection	874:882	arg1	down					910:913	down	910:913	down	910:913	Twenty six N-linked glycans could be identified from the digest of a standard glycoprotein ovalbumin at a concentration of 0.01 μg/μL, and the detection limit of glycans could be down to 1 ng/μL with 21 N-linked glycans identified.
29546983	4	1	theme	detection	874:882	arg1	limit					884:888	the detection limit	870:888	the detection limit of glycans	870:899	Twenty six N-linked glycans could be identified from the digest of a standard glycoprotein ovalbumin at a concentration of 0.01 μg/μL, and the detection limit of glycans could be down to 1 ng/μL with 21 N-linked glycans identified.
29546983	6	2	theme	carbon	1268:1273	arg1	enrichment					1234:1243	the enrichment	1230:1243	the enrichment of oxidized metal-free carbon	1230:1273	From a real complex sample of a healthy human serum, there were 43 N-linked glycans identified after the enrichment of oxidized metal-free carbon.
29546983	1	3	theme	metal-free	245:254	arg1	material					263:270	a facile and eco-friendly synthesized highly porous metal-free carbon material	193:270	a facile and eco-friendly synthesized highly porous metal-free carbon material	193:270	In this work, a highly efficient profiling of N-linked glycans was achieved by a facile and eco-friendly synthesized highly porous metal-free carbon material.
29546983	3	4	link	N-linked	585:592	arg1	glycans					594:600	N-linked glycans	585:600	N-linked glycans from complex biological samples due to the strong interaction between carbon and glycan as well as the size-exclusion mechanism	585:728	After further oxidation, the oxidized metal-free carbon was applied to the selective isolation of N-linked glycans from complex biological samples due to the strong interaction between carbon and glycan as well as the size-exclusion mechanism.
29546983	6	5	theme	oxidized	1248:1255	arg1	carbon					1268:1273	oxidized metal-free carbon	1248:1273	oxidized metal-free carbon	1248:1273	From a real complex sample of a healthy human serum, there were 43 N-linked glycans identified after the enrichment of oxidized metal-free carbon.
29546983	3	6	theme	oxidized	516:523	arg1	carbon					536:541	the oxidized metal-free carbon	512:541	the oxidized metal-free carbon	512:541	After further oxidation, the oxidized metal-free carbon was applied to the selective isolation of N-linked glycans from complex biological samples due to the strong interaction between carbon and glycan as well as the size-exclusion mechanism.
29546983	1	7	theme	carbon	256:261	arg1	material					263:270	a facile and eco-friendly synthesized highly porous metal-free carbon material	193:270	a facile and eco-friendly synthesized highly porous metal-free carbon material	193:270	In this work, a highly efficient profiling of N-linked glycans was achieved by a facile and eco-friendly synthesized highly porous metal-free carbon material.
29546983	0	8	theme	Glycans	105:111	arg1	Profiling					83:91	Profiling	83:91	Profiling of N-Linked Glycans	83:111	Highly Porous Metal-Free Graphitic Carbon Derived from Metal-Organic Framework for Profiling of N-Linked Glycans.
29546983	7	9	theme	new	1323:1325	arg1	prospect					1327:1334	new prospect	1323:1334	new prospect for the high-throughput identification of glycan	1323:1383	In a word, the metal-free carbon is opening up new prospect for the high-throughput identification of glycan.
29546983	4	10	from	digest	788:793	arg1	concentration					837:849	a concentration	835:849	a concentration of 0.01 μg/μL	835:863	Twenty six N-linked glycans could be identified from the digest of a standard glycoprotein ovalbumin at a concentration of 0.01 μg/μL, and the detection limit of glycans could be down to 1 ng/μL with 21 N-linked glycans identified.
29546983	4	11	link	N-linked	742:749	arg1	glycans					751:757	Twenty six N-linked glycans	731:757	Twenty six N-linked glycans	731:757	Twenty six N-linked glycans could be identified from the digest of a standard glycoprotein ovalbumin at a concentration of 0.01 μg/μL, and the detection limit of glycans could be down to 1 ng/μL with 21 N-linked glycans identified.
29546983	6	12	theme	N-linked	1196:1203	arg1	glycans					1205:1211	43 N-linked glycans	1193:1211	43 N-linked glycans identified after the enrichment of oxidized metal-free carbon	1193:1273	From a real complex sample of a healthy human serum, there were 43 N-linked glycans identified after the enrichment of oxidized metal-free carbon.
29546983	0	13	theme	N-Linked	96:103	arg1	Glycans					105:111	N-Linked Glycans	96:111	N-Linked Glycans	96:111	Highly Porous Metal-Free Graphitic Carbon Derived from Metal-Organic Framework for Profiling of N-Linked Glycans.
29546983	4	14	theme	N-linked	742:749	arg1	glycans					751:757	Twenty six N-linked glycans	731:757	Twenty six N-linked glycans	731:757	Twenty six N-linked glycans could be identified from the digest of a standard glycoprotein ovalbumin at a concentration of 0.01 μg/μL, and the detection limit of glycans could be down to 1 ng/μL with 21 N-linked glycans identified.
29546983	3	15	from	samples	626:632	arg1	isolation					572:580	the selective isolation	558:580	the selective isolation of N-linked glycans from complex biological samples due to the strong interaction between carbon and glycan as well as the size-exclusion mechanism	558:728	After further oxidation, the oxidized metal-free carbon was applied to the selective isolation of N-linked glycans from complex biological samples due to the strong interaction between carbon and glycan as well as the size-exclusion mechanism.
29546983	3	15	from	samples	626:632	arg1	glycans					594:600	N-linked glycans	585:600	N-linked glycans from complex biological samples due to the strong interaction between carbon and glycan as well as the size-exclusion mechanism	585:728	After further oxidation, the oxidized metal-free carbon was applied to the selective isolation of N-linked glycans from complex biological samples due to the strong interaction between carbon and glycan as well as the size-exclusion mechanism.
29546983	3	16	theme	further	493:499	arg1	oxidation					501:509	further oxidation	493:509	further oxidation	493:509	After further oxidation, the oxidized metal-free carbon was applied to the selective isolation of N-linked glycans from complex biological samples due to the strong interaction between carbon and glycan as well as the size-exclusion mechanism.
29546983	4	17	theme	μg/μL	859:863	arg1	concentration					837:849	a concentration	835:849	a concentration of 0.01 μg/μL	835:863	Twenty six N-linked glycans could be identified from the digest of a standard glycoprotein ovalbumin at a concentration of 0.01 μg/μL, and the detection limit of glycans could be down to 1 ng/μL with 21 N-linked glycans identified.
29546983	7	18	theme	glycan	1378:1383	arg1	identification					1360:1373	the high-throughput identification	1340:1373	the high-throughput identification of glycan	1340:1383	In a word, the metal-free carbon is opening up new prospect for the high-throughput identification of glycan.
29546983	2	19	theme	metal-free	277:286	arg1	carbon					288:293	The metal-free carbon	273:293	The metal-free carbon	273:293	The metal-free carbon was derived from a well-defined nanorod zinc metal-organic framework via the metal removal under a high-temperature carbonization, which exhibited a highly specific surface area of 1700 m2/g.
29546983	4	20	with	ng/μL	920:924	arg1	glycans					943:949	21 N-linked glycans	931:949	21 N-linked glycans identified	931:960	Twenty six N-linked glycans could be identified from the digest of a standard glycoprotein ovalbumin at a concentration of 0.01 μg/μL, and the detection limit of glycans could be down to 1 ng/μL with 21 N-linked glycans identified.
29546983	1	21	theme	N-linked	160:167	arg1	glycans					169:175	N-linked glycans	160:175	N-linked glycans	160:175	In this work, a highly efficient profiling of N-linked glycans was achieved by a facile and eco-friendly synthesized highly porous metal-free carbon material.
29546983	5	22	theme	mass	972:975	arg1	ratio					977:981	the mass ratio	968:981	the mass ratio of the interfering protein bovine serum albumin vs a standard ovalbumin digest	968:1060	When the mass ratio of the interfering protein bovine serum albumin vs a standard ovalbumin digest is up to 500:1, there were 24 N-glycans confidentially identified.
29546983	5	22	theme	mass	972:975	arg1	500:1					1071:1075	500:1	1071:1075	500:1	1071:1075	When the mass ratio of the interfering protein bovine serum albumin vs a standard ovalbumin digest is up to 500:1, there were 24 N-glycans confidentially identified.
29546983	7	23	from	opening	1312:1318	arg1	word					1281:1284	a word	1279:1284	a word	1279:1284	In a word, the metal-free carbon is opening up new prospect for the high-throughput identification of glycan.
29546983	1	24	theme	glycans	169:175	arg1	profiling					147:155	a highly efficient profiling	128:155	a highly efficient profiling of N-linked glycans	128:175	In this work, a highly efficient profiling of N-linked glycans was achieved by a facile and eco-friendly synthesized highly porous metal-free carbon material.
29546983	0	25	theme	Porous	7:12	arg1	Carbon					35:40	Highly Porous Metal-Free Graphitic Carbon	0:40	Highly Porous Metal-Free Graphitic Carbon	0:40	Highly Porous Metal-Free Graphitic Carbon Derived from Metal-Organic Framework for Profiling of N-Linked Glycans.
29546983	6	26	theme	metal-free	1257:1266	arg1	carbon					1268:1273	oxidized metal-free carbon	1248:1273	oxidized metal-free carbon	1248:1273	From a real complex sample of a healthy human serum, there were 43 N-linked glycans identified after the enrichment of oxidized metal-free carbon.
29546983	1	27	link	N-linked	160:167	arg1	glycans					169:175	N-linked glycans	160:175	N-linked glycans	160:175	In this work, a highly efficient profiling of N-linked glycans was achieved by a facile and eco-friendly synthesized highly porous metal-free carbon material.
29546983	2	28	theme	well-defined	314:325	arg1	framework					354:362	a well-defined nanorod zinc metal-organic framework	312:362	a well-defined nanorod zinc metal-organic framework	312:362	The metal-free carbon was derived from a well-defined nanorod zinc metal-organic framework via the metal removal under a high-temperature carbonization, which exhibited a highly specific surface area of 1700 m2/g.
29546983	5	29	dep	protein	1002:1008	arg1	albumin					1023:1029	bovine serum albumin	1010:1029	bovine serum albumin	1010:1029	When the mass ratio of the interfering protein bovine serum albumin vs a standard ovalbumin digest is up to 500:1, there were 24 N-glycans confidentially identified.
29546983	2	30	theme	surface	460:466	arg1	area					468:471	a highly specific surface area	442:471	a highly specific surface area of 1700 m2/g	442:484	The metal-free carbon was derived from a well-defined nanorod zinc metal-organic framework via the metal removal under a high-temperature carbonization, which exhibited a highly specific surface area of 1700 m2/g.
29546983	3	31	theme	N-linked	585:592	arg1	glycans					594:600	N-linked glycans	585:600	N-linked glycans from complex biological samples due to the strong interaction between carbon and glycan as well as the size-exclusion mechanism	585:728	After further oxidation, the oxidized metal-free carbon was applied to the selective isolation of N-linked glycans from complex biological samples due to the strong interaction between carbon and glycan as well as the size-exclusion mechanism.
29546983	6	32	theme	real	1136:1139	arg1	sample					1149:1154	a real complex sample	1134:1154	a real complex sample of a healthy human serum	1134:1179	From a real complex sample of a healthy human serum, there were 43 N-linked glycans identified after the enrichment of oxidized metal-free carbon.
29546983	0	33	theme	Graphitic	25:33	arg1	Carbon					35:40	Highly Porous Metal-Free Graphitic Carbon	0:40	Highly Porous Metal-Free Graphitic Carbon	0:40	Highly Porous Metal-Free Graphitic Carbon Derived from Metal-Organic Framework for Profiling of N-Linked Glycans.
29546983	6	34	theme	human	1169:1173	arg1	serum					1175:1179	a healthy human serum	1159:1179	a healthy human serum	1159:1179	From a real complex sample of a healthy human serum, there were 43 N-linked glycans identified after the enrichment of oxidized metal-free carbon.
29546983	3	35	theme	selective	562:570	arg1	isolation					572:580	the selective isolation	558:580	the selective isolation of N-linked glycans from complex biological samples due to the strong interaction between carbon and glycan as well as the size-exclusion mechanism	558:728	After further oxidation, the oxidized metal-free carbon was applied to the selective isolation of N-linked glycans from complex biological samples due to the strong interaction between carbon and glycan as well as the size-exclusion mechanism.
29546983	5	36	theme	protein	1002:1008	arg1	ratio					977:981	the mass ratio	968:981	the mass ratio of the interfering protein bovine serum albumin vs a standard ovalbumin digest	968:1060	When the mass ratio of the interfering protein bovine serum albumin vs a standard ovalbumin digest is up to 500:1, there were 24 N-glycans confidentially identified.
29546983	5	36	theme	protein	1002:1008	arg1	500:1					1071:1075	500:1	1071:1075	500:1	1071:1075	When the mass ratio of the interfering protein bovine serum albumin vs a standard ovalbumin digest is up to 500:1, there were 24 N-glycans confidentially identified.
29546983	0	37	theme	Metal-Free	14:23	arg1	Carbon					35:40	Highly Porous Metal-Free Graphitic Carbon	0:40	Highly Porous Metal-Free Graphitic Carbon	0:40	Highly Porous Metal-Free Graphitic Carbon Derived from Metal-Organic Framework for Profiling of N-Linked Glycans.
29546983	2	38	attach	derived	299:305	arg1	framework					354:362	a well-defined nanorod zinc metal-organic framework	312:362	a well-defined nanorod zinc metal-organic framework	312:362	The metal-free carbon was derived from a well-defined nanorod zinc metal-organic framework via the metal removal under a high-temperature carbonization, which exhibited a highly specific surface area of 1700 m2/g.
29546983	2	38	attach	derived	299:305	arg2	carbon					288:293	The metal-free carbon	273:293	The metal-free carbon	273:293	The metal-free carbon was derived from a well-defined nanorod zinc metal-organic framework via the metal removal under a high-temperature carbonization, which exhibited a highly specific surface area of 1700 m2/g.
29546983	5	39	theme	digest	1055:1060	arg1	ratio					977:981	the mass ratio	968:981	the mass ratio of the interfering protein bovine serum albumin vs a standard ovalbumin digest	968:1060	When the mass ratio of the interfering protein bovine serum albumin vs a standard ovalbumin digest is up to 500:1, there were 24 N-glycans confidentially identified.
29546983	5	39	theme	digest	1055:1060	arg1	500:1					1071:1075	500:1	1071:1075	500:1	1071:1075	When the mass ratio of the interfering protein bovine serum albumin vs a standard ovalbumin digest is up to 500:1, there were 24 N-glycans confidentially identified.
29546983	5	40	theme	serum	1017:1021	arg1	albumin					1023:1029	bovine serum albumin	1010:1029	bovine serum albumin	1010:1029	When the mass ratio of the interfering protein bovine serum albumin vs a standard ovalbumin digest is up to 500:1, there were 24 N-glycans confidentially identified.
29546983	5	41	theme	interfering	990:1000	arg1	protein					1002:1008	the interfering protein bovine serum albumin vs a standard ovalbumin digest	986:1060	protein	1002:1008	When the mass ratio of the interfering protein bovine serum albumin vs a standard ovalbumin digest is up to 500:1, there were 24 N-glycans confidentially identified.
29546983	3	42	theme	complex	607:613	arg1	samples					626:632	complex biological samples	607:632	complex biological samples due to the strong interaction between carbon and glycan as well as the size-exclusion mechanism	607:728	After further oxidation, the oxidized metal-free carbon was applied to the selective isolation of N-linked glycans from complex biological samples due to the strong interaction between carbon and glycan as well as the size-exclusion mechanism.
29546983	6	43	theme	healthy	1161:1167	arg1	serum					1175:1179	a healthy human serum	1159:1179	a healthy human serum	1159:1179	From a real complex sample of a healthy human serum, there were 43 N-linked glycans identified after the enrichment of oxidized metal-free carbon.
29546983	1	44	theme	facile	195:200	arg1	material					263:270	a facile and eco-friendly synthesized highly porous metal-free carbon material	193:270	a facile and eco-friendly synthesized highly porous metal-free carbon material	193:270	In this work, a highly efficient profiling of N-linked glycans was achieved by a facile and eco-friendly synthesized highly porous metal-free carbon material.
29546983	2	45	theme	m2/g	481:484	arg1	area					468:471	a highly specific surface area	442:471	a highly specific surface area of 1700 m2/g	442:484	The metal-free carbon was derived from a well-defined nanorod zinc metal-organic framework via the metal removal under a high-temperature carbonization, which exhibited a highly specific surface area of 1700 m2/g.
29546983	7	46	theme	high-throughput	1344:1358	arg1	identification					1360:1373	the high-throughput identification	1340:1373	the high-throughput identification of glycan	1340:1383	In a word, the metal-free carbon is opening up new prospect for the high-throughput identification of glycan.
29546983	3	47	theme	strong	645:650	arg1	interaction					652:662	the strong interaction	641:662	the strong interaction between carbon and glycan as well as the size-exclusion mechanism	641:728	After further oxidation, the oxidized metal-free carbon was applied to the selective isolation of N-linked glycans from complex biological samples due to the strong interaction between carbon and glycan as well as the size-exclusion mechanism.
29546983	2	48	theme	specific	451:458	arg1	area					468:471	a highly specific surface area	442:471	a highly specific surface area of 1700 m2/g	442:484	The metal-free carbon was derived from a well-defined nanorod zinc metal-organic framework via the metal removal under a high-temperature carbonization, which exhibited a highly specific surface area of 1700 m2/g.
29546983	0	49	theme	Metal-Organic	55:67	arg1	Framework					69:77	Metal-Organic Framework	55:77	Metal-Organic Framework for Profiling of N-Linked Glycans	55:111	Highly Porous Metal-Free Graphitic Carbon Derived from Metal-Organic Framework for Profiling of N-Linked Glycans.
29546983	4	50	theme	N-linked	934:941	arg1	glycans					943:949	21 N-linked glycans	931:949	21 N-linked glycans identified	931:960	Twenty six N-linked glycans could be identified from the digest of a standard glycoprotein ovalbumin at a concentration of 0.01 μg/μL, and the detection limit of glycans could be down to 1 ng/μL with 21 N-linked glycans identified.
29546983	3	51	theme	glycans	594:600	arg1	isolation					572:580	the selective isolation	558:580	the selective isolation of N-linked glycans from complex biological samples due to the strong interaction between carbon and glycan as well as the size-exclusion mechanism	558:728	After further oxidation, the oxidized metal-free carbon was applied to the selective isolation of N-linked glycans from complex biological samples due to the strong interaction between carbon and glycan as well as the size-exclusion mechanism.
29546983	4	52	from	concentration	837:849	arg1	ovalbumin					822:830	a standard glycoprotein ovalbumin	798:830	a standard glycoprotein ovalbumin at a concentration of 0.01 μg/μL	798:863	Twenty six N-linked glycans could be identified from the digest of a standard glycoprotein ovalbumin at a concentration of 0.01 μg/μL, and the detection limit of glycans could be down to 1 ng/μL with 21 N-linked glycans identified.
29546983	4	52	from	concentration	837:849	arg1	digest					788:793	the digest	784:793	the digest of a standard glycoprotein ovalbumin at a concentration of 0.01 μg/μL	784:863	Twenty six N-linked glycans could be identified from the digest of a standard glycoprotein ovalbumin at a concentration of 0.01 μg/μL, and the detection limit of glycans could be down to 1 ng/μL with 21 N-linked glycans identified.
29546983	2	53	theme	metal	372:376	arg1	removal					378:384	the metal removal	368:384	the metal removal under a high-temperature carbonization, which exhibited a highly specific surface area of 1700 m2/g	368:484	The metal-free carbon was derived from a well-defined nanorod zinc metal-organic framework via the metal removal under a high-temperature carbonization, which exhibited a highly specific surface area of 1700 m2/g.
29546983	5	54	dep	500:1	1071:1075	arg1	up					1065:1066	up	1065:1066	up	1065:1066	When the mass ratio of the interfering protein bovine serum albumin vs a standard ovalbumin digest is up to 500:1, there were 24 N-glycans confidentially identified.
29546983	4	55	theme	glycans	893:899	arg1	down					910:913	down	910:913	down	910:913	Twenty six N-linked glycans could be identified from the digest of a standard glycoprotein ovalbumin at a concentration of 0.01 μg/μL, and the detection limit of glycans could be down to 1 ng/μL with 21 N-linked glycans identified.
29546983	4	55	theme	glycans	893:899	arg1	limit					884:888	the detection limit	870:888	the detection limit of glycans	870:899	Twenty six N-linked glycans could be identified from the digest of a standard glycoprotein ovalbumin at a concentration of 0.01 μg/μL, and the detection limit of glycans could be down to 1 ng/μL with 21 N-linked glycans identified.
29546983	7	56	theme	metal-free	1291:1300	arg1	opening					1312:1318	opening	1312:1318	opening up new prospect for the high-throughput identification of glycan	1312:1383	In a word, the metal-free carbon is opening up new prospect for the high-throughput identification of glycan.
29546983	7	56	theme	metal-free	1291:1300	arg1	carbon					1302:1307	the metal-free carbon	1287:1307	the metal-free carbon	1287:1307	In a word, the metal-free carbon is opening up new prospect for the high-throughput identification of glycan.
29546983	2	57	theme	metal-organic	340:352	arg1	framework					354:362	a well-defined nanorod zinc metal-organic framework	312:362	a well-defined nanorod zinc metal-organic framework	312:362	The metal-free carbon was derived from a well-defined nanorod zinc metal-organic framework via the metal removal under a high-temperature carbonization, which exhibited a highly specific surface area of 1700 m2/g.
29546983	4	58	theme	glycoprotein	809:820	arg1	ovalbumin					822:830	a standard glycoprotein ovalbumin	798:830	a standard glycoprotein ovalbumin at a concentration of 0.01 μg/μL	798:863	Twenty six N-linked glycans could be identified from the digest of a standard glycoprotein ovalbumin at a concentration of 0.01 μg/μL, and the detection limit of glycans could be down to 1 ng/μL with 21 N-linked glycans identified.
29546983	4	59	link	N-linked	934:941	arg1	glycans					943:949	21 N-linked glycans	931:949	21 N-linked glycans identified	931:960	Twenty six N-linked glycans could be identified from the digest of a standard glycoprotein ovalbumin at a concentration of 0.01 μg/μL, and the detection limit of glycans could be down to 1 ng/μL with 21 N-linked glycans identified.
29546983	5	60	theme	bovine	1010:1015	arg1	albumin					1023:1029	bovine serum albumin	1010:1029	bovine serum albumin	1010:1029	When the mass ratio of the interfering protein bovine serum albumin vs a standard ovalbumin digest is up to 500:1, there were 24 N-glycans confidentially identified.
29546983	4	61	theme	standard	800:807	arg1	ovalbumin					822:830	a standard glycoprotein ovalbumin	798:830	a standard glycoprotein ovalbumin at a concentration of 0.01 μg/μL	798:863	Twenty six N-linked glycans could be identified from the digest of a standard glycoprotein ovalbumin at a concentration of 0.01 μg/μL, and the detection limit of glycans could be down to 1 ng/μL with 21 N-linked glycans identified.
29546983	3	62	theme	biological	615:624	arg1	samples					626:632	complex biological samples	607:632	complex biological samples due to the strong interaction between carbon and glycan as well as the size-exclusion mechanism	607:728	After further oxidation, the oxidized metal-free carbon was applied to the selective isolation of N-linked glycans from complex biological samples due to the strong interaction between carbon and glycan as well as the size-exclusion mechanism.
29546983	2	63	theme	zinc	335:338	arg1	framework					354:362	a well-defined nanorod zinc metal-organic framework	312:362	a well-defined nanorod zinc metal-organic framework	312:362	The metal-free carbon was derived from a well-defined nanorod zinc metal-organic framework via the metal removal under a high-temperature carbonization, which exhibited a highly specific surface area of 1700 m2/g.
29546983	3	64	from	isolation	572:580	arg1	samples					626:632	complex biological samples	607:632	complex biological samples due to the strong interaction between carbon and glycan as well as the size-exclusion mechanism	607:728	After further oxidation, the oxidized metal-free carbon was applied to the selective isolation of N-linked glycans from complex biological samples due to the strong interaction between carbon and glycan as well as the size-exclusion mechanism.
29546983	6	65	theme	complex	1141:1147	arg1	sample					1149:1154	a real complex sample	1134:1154	a real complex sample of a healthy human serum	1134:1179	From a real complex sample of a healthy human serum, there were 43 N-linked glycans identified after the enrichment of oxidized metal-free carbon.
29546983	5	66	theme	standard	1036:1043	arg1	digest					1055:1060	a standard ovalbumin digest	1034:1060	a standard ovalbumin digest	1034:1060	When the mass ratio of the interfering protein bovine serum albumin vs a standard ovalbumin digest is up to 500:1, there were 24 N-glycans confidentially identified.
29546983	6	67	theme	serum	1175:1179	arg1	sample					1149:1154	a real complex sample	1134:1154	a real complex sample of a healthy human serum	1134:1179	From a real complex sample of a healthy human serum, there were 43 N-linked glycans identified after the enrichment of oxidized metal-free carbon.
29546983	2	68	theme	nanorod	327:333	arg1	framework					354:362	a well-defined nanorod zinc metal-organic framework	312:362	a well-defined nanorod zinc metal-organic framework	312:362	The metal-free carbon was derived from a well-defined nanorod zinc metal-organic framework via the metal removal under a high-temperature carbonization, which exhibited a highly specific surface area of 1700 m2/g.
29546983	1	69	theme	synthesized	219:229	arg1	material					263:270	a facile and eco-friendly synthesized highly porous metal-free carbon material	193:270	a facile and eco-friendly synthesized highly porous metal-free carbon material	193:270	In this work, a highly efficient profiling of N-linked glycans was achieved by a facile and eco-friendly synthesized highly porous metal-free carbon material.
29546983	5	70	theme	ovalbumin	1045:1053	arg1	digest					1055:1060	a standard ovalbumin digest	1034:1060	a standard ovalbumin digest	1034:1060	When the mass ratio of the interfering protein bovine serum albumin vs a standard ovalbumin digest is up to 500:1, there were 24 N-glycans confidentially identified.
29546983	4	71	theme	ovalbumin	822:830	arg1	digest					788:793	the digest	784:793	the digest of a standard glycoprotein ovalbumin at a concentration of 0.01 μg/μL	784:863	Twenty six N-linked glycans could be identified from the digest of a standard glycoprotein ovalbumin at a concentration of 0.01 μg/μL, and the detection limit of glycans could be down to 1 ng/μL with 21 N-linked glycans identified.
29546983	3	72	theme	metal-free	525:534	arg1	carbon					536:541	the oxidized metal-free carbon	512:541	the oxidized metal-free carbon	512:541	After further oxidation, the oxidized metal-free carbon was applied to the selective isolation of N-linked glycans from complex biological samples due to the strong interaction between carbon and glycan as well as the size-exclusion mechanism.
29546983	2	73	theme	high-temperature	394:409	arg1	carbonization					411:423	a high-temperature carbonization	392:423	a high-temperature carbonization	392:423	The metal-free carbon was derived from a well-defined nanorod zinc metal-organic framework via the metal removal under a high-temperature carbonization, which exhibited a highly specific surface area of 1700 m2/g.
29546983	4	74	gly	glycoprotein	809:820	arg1	glycoprotein					809:820	a standard glycoprotein ovalbumin	798:830	a standard glycoprotein ovalbumin at a concentration of 0.01 μg/μL	798:863	Twenty six N-linked glycans could be identified from the digest of a standard glycoprotein ovalbumin at a concentration of 0.01 μg/μL, and the detection limit of glycans could be down to 1 ng/μL with 21 N-linked glycans identified.
29546983	3	75	theme	size-exclusion	705:718	arg1	mechanism					720:728	the size-exclusion mechanism	701:728	the strong interaction between carbon and glycan as well as the size-exclusion mechanism	641:728	After further oxidation, the oxidized metal-free carbon was applied to the selective isolation of N-linked glycans from complex biological samples due to the strong interaction between carbon and glycan as well as the size-exclusion mechanism.
29546983	3	76	theme	due	634:636	arg1	samples					626:632	complex biological samples	607:632	complex biological samples due to the strong interaction between carbon and glycan as well as the size-exclusion mechanism	607:728	After further oxidation, the oxidized metal-free carbon was applied to the selective isolation of N-linked glycans from complex biological samples due to the strong interaction between carbon and glycan as well as the size-exclusion mechanism.
29546983	1	77	theme	efficient	137:145	arg1	profiling					147:155	a highly efficient profiling	128:155	a highly efficient profiling of N-linked glycans	128:175	In this work, a highly efficient profiling of N-linked glycans was achieved by a facile and eco-friendly synthesized highly porous metal-free carbon material.
29546983	1	78	theme	porous	238:243	arg1	material					263:270	a facile and eco-friendly synthesized highly porous metal-free carbon material	193:270	a facile and eco-friendly synthesized highly porous metal-free carbon material	193:270	In this work, a highly efficient profiling of N-linked glycans was achieved by a facile and eco-friendly synthesized highly porous metal-free carbon material.
30947244	4	0	gly	glycoproteins	728:740	arg1	glycoproteins					728:740	fungal glycoproteins	721:740	fungal glycoproteins	721:740	Each of these domains can bind to chitin, to N-linked oligosaccharides in human skin glycoproteins, and to N-linked oligosaccharides on fungal glycoproteins.
30947244	8	1	theme	pathogen	1098:1105	arg1	adhesion					1107:1114	pathogen adhesion	1098:1114	pathogen adhesion to human skin	1098:1128	Third, the LysM proteins could help facilitate pathogen adhesion to human skin.
30947244	0	2	attach	present	103:109	arg2	oligosaccharides					86:101	the N-linked oligosaccharides	73:101	the N-linked oligosaccharides present on human skin glycoproteins	73:137	Trichophyton rubrum LysM proteins bind to fungal cell wall chitin and to the N-linked oligosaccharides present on human skin glycoproteins.
30947244	0	2	attach	present	103:109	arg1	glycoproteins					125:137	human skin glycoproteins	114:137	human skin glycoproteins	114:137	Trichophyton rubrum LysM proteins bind to fungal cell wall chitin and to the N-linked oligosaccharides present on human skin glycoproteins.
30947244	1	3	theme	Trichophyton	144:155	arg1	genome					164:169	The Trichophyton rubrum genome	140:169	The Trichophyton rubrum genome	140:169	The Trichophyton rubrum genome contains six proteins containing two or more lysin M (LysM) domains.
30947244	4	4	from	oligosaccharides	639:654	arg1	glycoproteins					670:682	human skin glycoproteins	659:682	human skin glycoproteins	659:682	Each of these domains can bind to chitin, to N-linked oligosaccharides in human skin glycoproteins, and to N-linked oligosaccharides on fungal glycoproteins.
30947244	3	5	contain	contains	547:554	arg1	protein					539:545	the protein	535:545	the protein	535:545	We have characterized the individual LysM domains in LysM1, and shown that the protein contains two functional LysM domains.
30947244	3	5	contain	contains	547:554	arg2	domains					576:582	two functional LysM domains	556:582	two functional LysM domains	556:582	We have characterized the individual LysM domains in LysM1, and shown that the protein contains two functional LysM domains.
30947244	7	6	theme	immune	1030:1035	arg1	surveillance					1037:1048	immune surveillance	1030:1048	immune surveillance	1030:1048	Second, the LysM proteins could shield the pathogen's glycoproteins from host degradation and immune surveillance.
30947244	8	7	theme	human	1119:1123	arg1	skin					1125:1128	human skin	1119:1128	human skin	1119:1128	Third, the LysM proteins could help facilitate pathogen adhesion to human skin.
30947244	1	8	theme	rubrum	157:162	arg1	genome					164:169	The Trichophyton rubrum genome	140:169	The Trichophyton rubrum genome	140:169	The Trichophyton rubrum genome contains six proteins containing two or more lysin M (LysM) domains.
30947244	0	9	theme	N-linked	77:84	arg1	oligosaccharides					86:101	the N-linked oligosaccharides	73:101	the N-linked oligosaccharides present on human skin glycoproteins	73:137	Trichophyton rubrum LysM proteins bind to fungal cell wall chitin and to the N-linked oligosaccharides present on human skin glycoproteins.
30947244	4	10	theme	fungal	721:726	arg1	glycoproteins					728:740	fungal glycoproteins	721:740	fungal glycoproteins	721:740	Each of these domains can bind to chitin, to N-linked oligosaccharides in human skin glycoproteins, and to N-linked oligosaccharides on fungal glycoproteins.
30947244	4	11	link	N-linked	692:699	arg1	oligosaccharides					701:716	N-linked oligosaccharides	692:716	N-linked oligosaccharides on fungal glycoproteins	692:740	Each of these domains can bind to chitin, to N-linked oligosaccharides in human skin glycoproteins, and to N-linked oligosaccharides on fungal glycoproteins.
30947244	7	12	gly	glycoproteins	990:1002	arg1	glycoproteins					990:1002	the pathogen's glycoproteins	975:1002	the pathogen's glycoproteins	975:1002	Second, the LysM proteins could shield the pathogen's glycoproteins from host degradation and immune surveillance.
30947244	6	13	theme	LysM	857:860	arg1	proteins					862:869	the T. rubrum LysM proteins	843:869	the T. rubrum LysM proteins	843:869	First, the T. rubrum LysM proteins could shield host cell wall chitin from the human immune system.
30947244	4	14	link	N-linked	630:637	arg1	oligosaccharides					639:654	N-linked oligosaccharides	630:654	N-linked oligosaccharides in human skin glycoproteins	630:682	Each of these domains can bind to chitin, to N-linked oligosaccharides in human skin glycoproteins, and to N-linked oligosaccharides on fungal glycoproteins.
30947244	0	15	theme	present	103:109	arg1	oligosaccharides					86:101	the N-linked oligosaccharides	73:101	the N-linked oligosaccharides present on human skin glycoproteins	73:137	Trichophyton rubrum LysM proteins bind to fungal cell wall chitin and to the N-linked oligosaccharides present on human skin glycoproteins.
30947244	6	16	theme	rubrum	850:855	arg1	proteins					862:869	the T. rubrum LysM proteins	843:869	the T. rubrum LysM proteins	843:869	First, the T. rubrum LysM proteins could shield host cell wall chitin from the human immune system.
30947244	2	17	link	N-linked	392:399	arg1	oligosaccharides					401:416	N-linked oligosaccharides	392:416	N-linked oligosaccharides associated with human skin glycoproteins	392:457	We have characterized two of these proteins, LysM1 and LysM2, and demonstrated that these proteins have the capacity to bind two substrates, chitin and N-linked oligosaccharides associated with human skin glycoproteins.
30947244	2	17	link	N-linked	392:399	arg1	substrates					369:378	two substrates	365:378	two substrates	365:378	We have characterized two of these proteins, LysM1 and LysM2, and demonstrated that these proteins have the capacity to bind two substrates, chitin and N-linked oligosaccharides associated with human skin glycoproteins.
30947244	5	18	theme	LysM	763:766	arg1	proteins					768:775	LysM proteins	763:775	LysM proteins	763:775	We hypothesize that LysM proteins could provide the pathogen with three important functions.
30947244	6	19	theme	T.	847:848	arg1	proteins					862:869	the T. rubrum LysM proteins	843:869	the T. rubrum LysM proteins	843:869	First, the T. rubrum LysM proteins could shield host cell wall chitin from the human immune system.
30947244	4	20	from	oligosaccharides	701:716	arg1	glycoproteins					728:740	fungal glycoproteins	721:740	fungal glycoproteins	721:740	Each of these domains can bind to chitin, to N-linked oligosaccharides in human skin glycoproteins, and to N-linked oligosaccharides on fungal glycoproteins.
30947244	2	21	theme	N-linked	392:399	arg1	oligosaccharides					401:416	N-linked oligosaccharides	392:416	N-linked oligosaccharides associated with human skin glycoproteins	392:457	We have characterized two of these proteins, LysM1 and LysM2, and demonstrated that these proteins have the capacity to bind two substrates, chitin and N-linked oligosaccharides associated with human skin glycoproteins.
30947244	2	21	theme	N-linked	392:399	arg1	substrates					369:378	two substrates	365:378	two substrates	365:378	We have characterized two of these proteins, LysM1 and LysM2, and demonstrated that these proteins have the capacity to bind two substrates, chitin and N-linked oligosaccharides associated with human skin glycoproteins.
30947244	0	22	theme	rubrum	13:18	arg1	proteins					25:32	Trichophyton rubrum LysM proteins	0:32	Trichophyton rubrum LysM proteins	0:32	Trichophyton rubrum LysM proteins bind to fungal cell wall chitin and to the N-linked oligosaccharides present on human skin glycoproteins.
30947244	5	23	with	pathogen	795:802	arg1	functions					825:833	three important functions	809:833	three important functions	809:833	We hypothesize that LysM proteins could provide the pathogen with three important functions.
30947244	0	24	theme	Trichophyton	0:11	arg1	rubrum					13:18	Trichophyton rubrum	0:18	Trichophyton rubrum LysM proteins	0:32	Trichophyton rubrum LysM proteins bind to fungal cell wall chitin and to the N-linked oligosaccharides present on human skin glycoproteins.
30947244	7	25	theme	host	1009:1012	arg1	degradation					1014:1024	host degradation	1009:1024	host degradation	1009:1024	Second, the LysM proteins could shield the pathogen's glycoproteins from host degradation and immune surveillance.
30947244	6	26	theme	immune	921:926	arg1	system					928:933	the human immune system	911:933	the human immune system	911:933	First, the T. rubrum LysM proteins could shield host cell wall chitin from the human immune system.
30947244	0	27	theme	human	114:118	arg1	glycoproteins					125:137	human skin glycoproteins	114:137	human skin glycoproteins	114:137	Trichophyton rubrum LysM proteins bind to fungal cell wall chitin and to the N-linked oligosaccharides present on human skin glycoproteins.
30947244	2	28	gly	glycoproteins	445:457	arg1	glycoproteins					445:457	human skin glycoproteins	434:457	human skin glycoproteins	434:457	We have characterized two of these proteins, LysM1 and LysM2, and demonstrated that these proteins have the capacity to bind two substrates, chitin and N-linked oligosaccharides associated with human skin glycoproteins.
30947244	0	29	theme	LysM	20:23	arg1	proteins					25:32	Trichophyton rubrum LysM proteins	0:32	Trichophyton rubrum LysM proteins	0:32	Trichophyton rubrum LysM proteins bind to fungal cell wall chitin and to the N-linked oligosaccharides present on human skin glycoproteins.
30947244	6	30	theme	human	915:919	arg1	system					928:933	the human immune system	911:933	the human immune system	911:933	First, the T. rubrum LysM proteins could shield host cell wall chitin from the human immune system.
30947244	2	31	contain	have	339:342	arg2	capacity					348:355	the capacity to bind two substrates, chitin and N-linked oligosaccharides associated with human skin glycoproteins	344:457	the capacity to bind two substrates, chitin and N-linked oligosaccharides associated with human skin glycoproteins	344:457	We have characterized two of these proteins, LysM1 and LysM2, and demonstrated that these proteins have the capacity to bind two substrates, chitin and N-linked oligosaccharides associated with human skin glycoproteins.
30947244	2	31	contain	have	339:342	arg1	proteins					330:337	these proteins	324:337	these proteins	324:337	We have characterized two of these proteins, LysM1 and LysM2, and demonstrated that these proteins have the capacity to bind two substrates, chitin and N-linked oligosaccharides associated with human skin glycoproteins.
30947244	4	32	theme	N-linked	692:699	arg1	oligosaccharides					701:716	N-linked oligosaccharides	692:716	N-linked oligosaccharides on fungal glycoproteins	692:740	Each of these domains can bind to chitin, to N-linked oligosaccharides in human skin glycoproteins, and to N-linked oligosaccharides on fungal glycoproteins.
30947244	4	33	theme	N-linked	630:637	arg1	oligosaccharides					639:654	N-linked oligosaccharides	630:654	N-linked oligosaccharides in human skin glycoproteins	630:682	Each of these domains can bind to chitin, to N-linked oligosaccharides in human skin glycoproteins, and to N-linked oligosaccharides on fungal glycoproteins.
30947244	2	34	theme	skin	440:443	arg1	glycoproteins					445:457	human skin glycoproteins	434:457	human skin glycoproteins	434:457	We have characterized two of these proteins, LysM1 and LysM2, and demonstrated that these proteins have the capacity to bind two substrates, chitin and N-linked oligosaccharides associated with human skin glycoproteins.
30947244	4	35	gly	glycoproteins	670:682	arg1	glycoproteins					670:682	human skin glycoproteins	659:682	human skin glycoproteins	659:682	Each of these domains can bind to chitin, to N-linked oligosaccharides in human skin glycoproteins, and to N-linked oligosaccharides on fungal glycoproteins.
30947244	2	36	theme	human	434:438	arg1	glycoproteins					445:457	human skin glycoproteins	434:457	human skin glycoproteins	434:457	We have characterized two of these proteins, LysM1 and LysM2, and demonstrated that these proteins have the capacity to bind two substrates, chitin and N-linked oligosaccharides associated with human skin glycoproteins.
30947244	0	37	theme	skin	120:123	arg1	glycoproteins					125:137	human skin glycoproteins	114:137	human skin glycoproteins	114:137	Trichophyton rubrum LysM proteins bind to fungal cell wall chitin and to the N-linked oligosaccharides present on human skin glycoproteins.
30947244	1	38	contain	contains	171:178	arg1	genome					164:169	The Trichophyton rubrum genome	140:169	The Trichophyton rubrum genome	140:169	The Trichophyton rubrum genome contains six proteins containing two or more lysin M (LysM) domains.
30947244	1	38	contain	contains	171:178	arg2	proteins					184:191	six proteins	180:191	six proteins containing two or more lysin M (LysM) domains	180:237	The Trichophyton rubrum genome contains six proteins containing two or more lysin M (LysM) domains.
30947244	3	39	theme	functional	560:569	arg1	domains					576:582	two functional LysM domains	556:582	two functional LysM domains	556:582	We have characterized the individual LysM domains in LysM1, and shown that the protein contains two functional LysM domains.
30947244	5	40	theme	important	815:823	arg1	functions					825:833	three important functions	809:833	three important functions	809:833	We hypothesize that LysM proteins could provide the pathogen with three important functions.
30947244	0	41	theme	cell	49:52	arg1	chitin					59:64	fungal cell wall chitin	42:64	fungal cell wall chitin	42:64	Trichophyton rubrum LysM proteins bind to fungal cell wall chitin and to the N-linked oligosaccharides present on human skin glycoproteins.
30947244	4	42	theme	skin	665:668	arg1	glycoproteins					670:682	human skin glycoproteins	659:682	human skin glycoproteins	659:682	Each of these domains can bind to chitin, to N-linked oligosaccharides in human skin glycoproteins, and to N-linked oligosaccharides on fungal glycoproteins.
30947244	0	43	theme	fungal	42:47	arg1	chitin					59:64	fungal cell wall chitin	42:64	fungal cell wall chitin	42:64	Trichophyton rubrum LysM proteins bind to fungal cell wall chitin and to the N-linked oligosaccharides present on human skin glycoproteins.
30947244	6	44	theme	wall	894:897	arg1	chitin					899:904	host cell wall chitin	884:904	host cell wall chitin	884:904	First, the T. rubrum LysM proteins could shield host cell wall chitin from the human immune system.
30947244	7	45	theme	LysM	948:951	arg1	proteins					953:960	the LysM proteins	944:960	the LysM proteins	944:960	Second, the LysM proteins could shield the pathogen's glycoproteins from host degradation and immune surveillance.
30947244	4	46	theme	human	659:663	arg1	glycoproteins					670:682	human skin glycoproteins	659:682	human skin glycoproteins	659:682	Each of these domains can bind to chitin, to N-linked oligosaccharides in human skin glycoproteins, and to N-linked oligosaccharides on fungal glycoproteins.
30947244	6	47	theme	cell	889:892	arg1	chitin					899:904	host cell wall chitin	884:904	host cell wall chitin	884:904	First, the T. rubrum LysM proteins could shield host cell wall chitin from the human immune system.
30947244	2	48	theme	proteins	275:282	arg1	two					262:264	two	262:264	two	262:264	We have characterized two of these proteins, LysM1 and LysM2, and demonstrated that these proteins have the capacity to bind two substrates, chitin and N-linked oligosaccharides associated with human skin glycoproteins.
30947244	2	48	theme	proteins	275:282	arg1	proteins					275:282	these proteins	269:282	these proteins	269:282	We have characterized two of these proteins, LysM1 and LysM2, and demonstrated that these proteins have the capacity to bind two substrates, chitin and N-linked oligosaccharides associated with human skin glycoproteins.
30947244	2	48	theme	proteins	275:282	arg1	LysM2					295:299	LysM2	295:299	LysM2	295:299	We have characterized two of these proteins, LysM1 and LysM2, and demonstrated that these proteins have the capacity to bind two substrates, chitin and N-linked oligosaccharides associated with human skin glycoproteins.
30947244	2	48	theme	proteins	275:282	arg1	LysM1					285:289	LysM1	285:289	LysM1	285:289	We have characterized two of these proteins, LysM1 and LysM2, and demonstrated that these proteins have the capacity to bind two substrates, chitin and N-linked oligosaccharides associated with human skin glycoproteins.
30947244	1	49	theme	lysin	216:220	arg1	domains					231:237	two or more lysin M (LysM) domains	204:237	two or more lysin M (LysM) domains	204:237	The Trichophyton rubrum genome contains six proteins containing two or more lysin M (LysM) domains.
30947244	8	50	theme	LysM	1062:1065	arg1	proteins					1067:1074	the LysM proteins	1058:1074	the LysM proteins	1058:1074	Third, the LysM proteins could help facilitate pathogen adhesion to human skin.
30947244	0	51	gly	glycoproteins	125:137	arg1	glycoproteins					125:137	human skin glycoproteins	114:137	human skin glycoproteins	114:137	Trichophyton rubrum LysM proteins bind to fungal cell wall chitin and to the N-linked oligosaccharides present on human skin glycoproteins.
30947244	1	52	theme	M	222:222	arg1	domains					231:237	two or more lysin M (LysM) domains	204:237	two or more lysin M (LysM) domains	204:237	The Trichophyton rubrum genome contains six proteins containing two or more lysin M (LysM) domains.
30947244	1	53	contain	containing	193:202	arg2	domains					231:237	two or more lysin M (LysM) domains	204:237	two or more lysin M (LysM) domains	204:237	The Trichophyton rubrum genome contains six proteins containing two or more lysin M (LysM) domains.
30947244	1	53	contain	containing	193:202	arg1	proteins					184:191	six proteins	180:191	six proteins containing two or more lysin M (LysM) domains	180:237	The Trichophyton rubrum genome contains six proteins containing two or more lysin M (LysM) domains.
30947244	3	54	theme	LysM	571:574	arg1	domains					576:582	two functional LysM domains	556:582	two functional LysM domains	556:582	We have characterized the individual LysM domains in LysM1, and shown that the protein contains two functional LysM domains.
30947244	3	55	theme	individual	486:495	arg1	domains					502:508	the individual LysM domains	482:508	the individual LysM domains in LysM1	482:517	We have characterized the individual LysM domains in LysM1, and shown that the protein contains two functional LysM domains.
30947244	0	56	theme	wall	54:57	arg1	chitin					59:64	fungal cell wall chitin	42:64	fungal cell wall chitin	42:64	Trichophyton rubrum LysM proteins bind to fungal cell wall chitin and to the N-linked oligosaccharides present on human skin glycoproteins.
30947244	6	57	theme	host	884:887	arg1	chitin					899:904	host cell wall chitin	884:904	host cell wall chitin	884:904	First, the T. rubrum LysM proteins could shield host cell wall chitin from the human immune system.
30947244	0	58	link	N-linked	77:84	arg1	oligosaccharides					86:101	the N-linked oligosaccharides	73:101	the N-linked oligosaccharides present on human skin glycoproteins	73:137	Trichophyton rubrum LysM proteins bind to fungal cell wall chitin and to the N-linked oligosaccharides present on human skin glycoproteins.
30947244	3	59	theme	LysM	497:500	arg1	domains					502:508	the individual LysM domains	482:508	the individual LysM domains in LysM1	482:517	We have characterized the individual LysM domains in LysM1, and shown that the protein contains two functional LysM domains.
30947244	3	60	from	domains	502:508	arg1	LysM1					513:517	LysM1	513:517	LysM1	513:517	We have characterized the individual LysM domains in LysM1, and shown that the protein contains two functional LysM domains.
30947244	1	61	theme	LysM	225:228	arg1	domains					231:237	two or more lysin M (LysM) domains	204:237	two or more lysin M (LysM) domains	204:237	The Trichophyton rubrum genome contains six proteins containing two or more lysin M (LysM) domains.
30745390	1	0	theme	self-produced	282:294	arg1	matrices					336:343	their self-produced extracellular polymeric substance (EPS) matrices	276:343	their self-produced extracellular polymeric substance (EPS) matrices	276:343	Pseudomonas aeruginosa colonizes the sputum of most adult cystic fibrosis patients, forming difficult-to-eradicate biofilms in which bacteria are protected in their self-produced extracellular polymeric substance (EPS) matrices.
30745390	6	1	theme	Maxwell	1224:1230	arg1	analyses					1232:1239	three-element Maxwell analyses	1210:1239	three-element Maxwell analyses	1210:1239	Low-load compression testing, followed by three-element Maxwell analyses, showed that the fastest relaxation component, associated with unbound water, was most important in LB-medium-grown biofilms.
30745390	1	2	theme	polymeric	310:318	arg1	matrices					336:343	their self-produced extracellular polymeric substance (EPS) matrices	276:343	their self-produced extracellular polymeric substance (EPS) matrices	276:343	Pseudomonas aeruginosa colonizes the sputum of most adult cystic fibrosis patients, forming difficult-to-eradicate biofilms in which bacteria are protected in their self-produced extracellular polymeric substance (EPS) matrices.
30745390	7	3	theme	biofilms	1518:1525	arg1	relaxation					1493:1502	the relaxation	1489:1502	the relaxation of ASM+-grown biofilms	1489:1525	Slower components due to water with dissolved polysaccharides, insoluble polysaccharides, and eDNA were most important in the relaxation of ASM+-grown biofilms.
30745390	10	4	theme	growth	2002:2007	arg1	media					2009:2013	growth media	2002:2013	growth media	2002:2013	In conclusion, stress relaxation of P. aeruginosa biofilms grown in different media revealed differences in matrix composition that, within the constraints of the antimicrobials and growth media applied, correlated with the matrix protection offered against different antimicrobials.
30745390	1	5	theme	patients	191:198	arg1	sputum					154:159	the sputum	150:159	the sputum of most adult cystic fibrosis patients	150:198	Pseudomonas aeruginosa colonizes the sputum of most adult cystic fibrosis patients, forming difficult-to-eradicate biofilms in which bacteria are protected in their self-produced extracellular polymeric substance (EPS) matrices.
30745390	7	6	from	relaxation	1493:1502	arg1	important					1476:1484	important	1476:1484	important	1476:1484	Slower components due to water with dissolved polysaccharides, insoluble polysaccharides, and eDNA were most important in the relaxation of ASM+-grown biofilms.
30745390	4	7	theme	aeruginosa	741:750	arg1	killing					727:733	killing	727:733	killing of P. aeruginosa	727:750	Since the viscoelasticity of biofilms has been related to antimicrobial penetration but not yet bacterial killing, this study aims to relate killing of P. aeruginosa, in its biofilm mode of growth, by three antimicrobials to biofilm viscoelasticity.
30745390	5	8	theme	sputum	948:953	arg1	ASM+					963:966	ASM+	963:966	ASM+	963:966	P. aeruginosa biofilms were grown for 18 h in a constant-depth film fermenter, with mucin-containing artificial sputum medium (ASM+), artificial sputum medium without mucin (ASM-), or Luria-Bertani (LB) broth; this yielded 100-μm-thick biofilms that differed in their amounts of matrix environmental DNA (eDNA) and polysaccharides.
30745390	5	8	theme	sputum	948:953	arg1	medium					955:960	mucin-containing artificial sputum medium	920:960	mucin-containing artificial sputum medium (ASM+)	920:967	P. aeruginosa biofilms were grown for 18 h in a constant-depth film fermenter, with mucin-containing artificial sputum medium (ASM+), artificial sputum medium without mucin (ASM-), or Luria-Bertani (LB) broth; this yielded 100-μm-thick biofilms that differed in their amounts of matrix environmental DNA (eDNA) and polysaccharides.
30745390	6	9	theme	compression	1177:1187	arg1	testing					1189:1195	Low-load compression testing	1168:1195	Low-load compression testing	1168:1195	Low-load compression testing, followed by three-element Maxwell analyses, showed that the fastest relaxation component, associated with unbound water, was most important in LB-medium-grown biofilms.
30745390	5	10	theme	film	899:902	arg1	fermenter					904:912	a constant-depth film fermenter	882:912	a constant-depth film fermenter	882:912	P. aeruginosa biofilms were grown for 18 h in a constant-depth film fermenter, with mucin-containing artificial sputum medium (ASM+), artificial sputum medium without mucin (ASM-), or Luria-Bertani (LB) broth; this yielded 100-μm-thick biofilms that differed in their amounts of matrix environmental DNA (eDNA) and polysaccharides.
30745390	7	11	theme	insoluble	1430:1438	arg1	polysaccharides					1440:1454	insoluble polysaccharides	1430:1454	insoluble polysaccharides	1430:1454	Slower components due to water with dissolved polysaccharides, insoluble polysaccharides, and eDNA were most important in the relaxation of ASM+-grown biofilms.
30745390	10	12	theme	biofilms	1870:1877	arg1	relaxation					1842:1851	stress relaxation	1835:1851	stress relaxation of P. aeruginosa biofilms grown in different media	1835:1902	In conclusion, stress relaxation of P. aeruginosa biofilms grown in different media revealed differences in matrix composition that, within the constraints of the antimicrobials and growth media applied, correlated with the matrix protection offered against different antimicrobials.
30745390	9	13	theme	most	1779:1782	arg1	polysaccharides					1794:1808	most insoluble polysaccharides	1779:1808	most insoluble polysaccharides	1779:1808	P. aeruginosa in LB-medium-grown biofilms was killed most by exposure to tobramycin, colistin, or an antimicrobial peptide, while ASM+ provided the most protective matrix, with less water and most insoluble polysaccharides and eDNA.
30745390	6	14	theme	fastest	1258:1264	arg1	important					1328:1336	important	1328:1336	important	1328:1336	Low-load compression testing, followed by three-element Maxwell analyses, showed that the fastest relaxation component, associated with unbound water, was most important in LB-medium-grown biofilms.
30745390	6	14	theme	fastest	1258:1264	arg1	component					1277:1285	the fastest relaxation component	1254:1285	the fastest relaxation component	1254:1285	Low-load compression testing, followed by three-element Maxwell analyses, showed that the fastest relaxation component, associated with unbound water, was most important in LB-medium-grown biofilms.
30745390	6	15	theme	LB-medium-grown	1341:1355	arg1	biofilms					1357:1364	LB-medium-grown biofilms	1341:1364	LB-medium-grown biofilms	1341:1364	Low-load compression testing, followed by three-element Maxwell analyses, showed that the fastest relaxation component, associated with unbound water, was most important in LB-medium-grown biofilms.
30745390	5	16	theme	artificial	970:979	arg1	medium					988:993	artificial sputum medium	970:993	artificial sputum medium without mucin (ASM-)	970:1014	P. aeruginosa biofilms were grown for 18 h in a constant-depth film fermenter, with mucin-containing artificial sputum medium (ASM+), artificial sputum medium without mucin (ASM-), or Luria-Bertani (LB) broth; this yielded 100-μm-thick biofilms that differed in their amounts of matrix environmental DNA (eDNA) and polysaccharides.
30745390	7	17	with	water	1392:1396	arg1	eDNA					1461:1464	eDNA	1461:1464	eDNA	1461:1464	Slower components due to water with dissolved polysaccharides, insoluble polysaccharides, and eDNA were most important in the relaxation of ASM+-grown biofilms.
30745390	7	17	with	water	1392:1396	arg1	polysaccharides					1413:1427	dissolved polysaccharides	1403:1427	dissolved polysaccharides	1403:1427	Slower components due to water with dissolved polysaccharides, insoluble polysaccharides, and eDNA were most important in the relaxation of ASM+-grown biofilms.
30745390	7	17	with	water	1392:1396	arg1	polysaccharides					1440:1454	insoluble polysaccharides	1430:1454	insoluble polysaccharides	1430:1454	Slower components due to water with dissolved polysaccharides, insoluble polysaccharides, and eDNA were most important in the relaxation of ASM+-grown biofilms.
30745390	1	18	theme	most	164:167	arg1	patients					191:198	most adult cystic fibrosis patients	164:198	most adult cystic fibrosis patients	164:198	Pseudomonas aeruginosa colonizes the sputum of most adult cystic fibrosis patients, forming difficult-to-eradicate biofilms in which bacteria are protected in their self-produced extracellular polymeric substance (EPS) matrices.
30745390	8	19	theme	intermediate	1555:1566	arg1	relaxation					1575:1584	intermediate stress relaxation	1555:1584	intermediate stress relaxation	1555:1584	ASM--grown biofilms showed intermediate stress relaxation.
30745390	4	20	theme	biofilm	760:766	arg1	mode					768:771	its biofilm mode	756:771	its biofilm mode of growth	756:781	Since the viscoelasticity of biofilms has been related to antimicrobial penetration but not yet bacterial killing, this study aims to relate killing of P. aeruginosa, in its biofilm mode of growth, by three antimicrobials to biofilm viscoelasticity.
30745390	0	21	theme	Grown	78:82	arg1	Biofilms					107:114	Differently Grown Pseudomonas aeruginosa Biofilms	66:114	Differently Grown Pseudomonas aeruginosa Biofilms	66:114	Role of Viscoelasticity in Bacterial Killing by Antimicrobials in Differently Grown Pseudomonas aeruginosa Biofilms.
30745390	5	22	theme	polysaccharides	1151:1165	arg1	amounts					1104:1110	their amounts	1098:1110	their amounts of matrix environmental DNA (eDNA) and polysaccharides	1098:1165	P. aeruginosa biofilms were grown for 18 h in a constant-depth film fermenter, with mucin-containing artificial sputum medium (ASM+), artificial sputum medium without mucin (ASM-), or Luria-Bertani (LB) broth; this yielded 100-μm-thick biofilms that differed in their amounts of matrix environmental DNA (eDNA) and polysaccharides.
30745390	5	22	theme	polysaccharides	1151:1165	arg1	eDNA					1141:1144	eDNA	1141:1144	eDNA	1141:1144	P. aeruginosa biofilms were grown for 18 h in a constant-depth film fermenter, with mucin-containing artificial sputum medium (ASM+), artificial sputum medium without mucin (ASM-), or Luria-Bertani (LB) broth; this yielded 100-μm-thick biofilms that differed in their amounts of matrix environmental DNA (eDNA) and polysaccharides.
30745390	5	22	theme	polysaccharides	1151:1165	arg1	polysaccharides					1151:1165	polysaccharides	1151:1165	polysaccharides	1151:1165	P. aeruginosa biofilms were grown for 18 h in a constant-depth film fermenter, with mucin-containing artificial sputum medium (ASM+), artificial sputum medium without mucin (ASM-), or Luria-Bertani (LB) broth; this yielded 100-μm-thick biofilms that differed in their amounts of matrix environmental DNA (eDNA) and polysaccharides.
30745390	5	22	theme	polysaccharides	1151:1165	arg1	DNA					1136:1138	matrix environmental DNA	1115:1138	matrix environmental DNA (eDNA)	1115:1145	P. aeruginosa biofilms were grown for 18 h in a constant-depth film fermenter, with mucin-containing artificial sputum medium (ASM+), artificial sputum medium without mucin (ASM-), or Luria-Bertani (LB) broth; this yielded 100-μm-thick biofilms that differed in their amounts of matrix environmental DNA (eDNA) and polysaccharides.
30745390	1	23	theme	cystic	175:180	arg1	fibrosis					182:189	cystic fibrosis	175:189	most adult cystic fibrosis patients	164:198	Pseudomonas aeruginosa colonizes the sputum of most adult cystic fibrosis patients, forming difficult-to-eradicate biofilms in which bacteria are protected in their self-produced extracellular polymeric substance (EPS) matrices.
30745390	0	24	from	Role	0:3	arg1	Killing					37:43	Bacterial Killing	27:43	Bacterial Killing by Antimicrobials in Differently Grown Pseudomonas aeruginosa Biofilms	27:114	Role of Viscoelasticity in Bacterial Killing by Antimicrobials in Differently Grown Pseudomonas aeruginosa Biofilms.
30745390	0	25	theme	aeruginosa	96:105	arg1	Biofilms					107:114	Differently Grown Pseudomonas aeruginosa Biofilms	66:114	Differently Grown Pseudomonas aeruginosa Biofilms	66:114	Role of Viscoelasticity in Bacterial Killing by Antimicrobials in Differently Grown Pseudomonas aeruginosa Biofilms.
30745390	5	26	theme	environmental	1122:1134	arg1	eDNA					1141:1144	eDNA	1141:1144	eDNA	1141:1144	P. aeruginosa biofilms were grown for 18 h in a constant-depth film fermenter, with mucin-containing artificial sputum medium (ASM+), artificial sputum medium without mucin (ASM-), or Luria-Bertani (LB) broth; this yielded 100-μm-thick biofilms that differed in their amounts of matrix environmental DNA (eDNA) and polysaccharides.
30745390	5	26	theme	environmental	1122:1134	arg1	DNA					1136:1138	matrix environmental DNA	1115:1138	matrix environmental DNA (eDNA)	1115:1145	P. aeruginosa biofilms were grown for 18 h in a constant-depth film fermenter, with mucin-containing artificial sputum medium (ASM+), artificial sputum medium without mucin (ASM-), or Luria-Bertani (LB) broth; this yielded 100-μm-thick biofilms that differed in their amounts of matrix environmental DNA (eDNA) and polysaccharides.
30745390	2	27	theme	multiple	478:485	arg1	constants					507:515	multiple characteristic time constants	478:515	multiple characteristic time constants	478:515	EPS provide biofilms with viscoelastic properties, causing time-dependent relaxation after stress-induced deformation, according to multiple characteristic time constants.
30745390	5	28	theme	Luria-Bertani	1020:1032	arg1	broth					1039:1043	Luria-Bertani (LB) broth	1020:1043	Luria-Bertani (LB) broth	1020:1043	P. aeruginosa biofilms were grown for 18 h in a constant-depth film fermenter, with mucin-containing artificial sputum medium (ASM+), artificial sputum medium without mucin (ASM-), or Luria-Bertani (LB) broth; this yielded 100-μm-thick biofilms that differed in their amounts of matrix environmental DNA (eDNA) and polysaccharides.
30745390	7	29	theme	due	1385:1387	arg1	components					1374:1383	Slower components	1367:1383	Slower components due to water with dissolved polysaccharides, insoluble polysaccharides, and eDNA	1367:1464	Slower components due to water with dissolved polysaccharides, insoluble polysaccharides, and eDNA were most important in the relaxation of ASM+-grown biofilms.
30745390	2	30	theme	stress-induced	437:450	arg1	deformation					452:462	stress-induced deformation	437:462	stress-induced deformation	437:462	EPS provide biofilms with viscoelastic properties, causing time-dependent relaxation after stress-induced deformation, according to multiple characteristic time constants.
30745390	1	31	theme	substance	320:328	arg1	matrices					336:343	their self-produced extracellular polymeric substance (EPS) matrices	276:343	their self-produced extracellular polymeric substance (EPS) matrices	276:343	Pseudomonas aeruginosa colonizes the sputum of most adult cystic fibrosis patients, forming difficult-to-eradicate biofilms in which bacteria are protected in their self-produced extracellular polymeric substance (EPS) matrices.
30745390	7	32	theme	Slower	1367:1372	arg1	components					1374:1383	Slower components	1367:1383	Slower components due to water with dissolved polysaccharides, insoluble polysaccharides, and eDNA	1367:1464	Slower components due to water with dissolved polysaccharides, insoluble polysaccharides, and eDNA were most important in the relaxation of ASM+-grown biofilms.
30745390	2	33	theme	characteristic	487:500	arg1	constants					507:515	multiple characteristic time constants	478:515	multiple characteristic time constants	478:515	EPS provide biofilms with viscoelastic properties, causing time-dependent relaxation after stress-induced deformation, according to multiple characteristic time constants.
30745390	0	34	theme	Viscoelasticity	8:22	arg1	Role					0:3	Role	0:3	Role of Viscoelasticity in Bacterial Killing by Antimicrobials in Differently Grown Pseudomonas aeruginosa Biofilms.	0:115	Role of Viscoelasticity in Bacterial Killing by Antimicrobials in Differently Grown Pseudomonas aeruginosa Biofilms.
30745390	1	35	theme	EPS	331:333	arg1	matrices					336:343	their self-produced extracellular polymeric substance (EPS) matrices	276:343	their self-produced extracellular polymeric substance (EPS) matrices	276:343	Pseudomonas aeruginosa colonizes the sputum of most adult cystic fibrosis patients, forming difficult-to-eradicate biofilms in which bacteria are protected in their self-produced extracellular polymeric substance (EPS) matrices.
30745390	10	36	theme	matrix	1928:1933	arg1	composition					1935:1945	matrix composition	1928:1945	matrix composition	1928:1945	In conclusion, stress relaxation of P. aeruginosa biofilms grown in different media revealed differences in matrix composition that, within the constraints of the antimicrobials and growth media applied, correlated with the matrix protection offered against different antimicrobials.
30745390	3	37	theme	time	524:527	arg1	constants					529:537	These time constants	518:537	These time constants	518:537	These time constants reflect different biofilm (matrix) components.
30745390	0	38	theme	Bacterial	27:35	arg1	Killing					37:43	Bacterial Killing	27:43	Bacterial Killing by Antimicrobials in Differently Grown Pseudomonas aeruginosa Biofilms	27:114	Role of Viscoelasticity in Bacterial Killing by Antimicrobials in Differently Grown Pseudomonas aeruginosa Biofilms.
30745390	3	39	theme	matrix	566:571	arg1	components					574:583	different biofilm (matrix) components	547:583	different biofilm (matrix) components	547:583	These time constants reflect different biofilm (matrix) components.
30745390	6	40	from	biofilms	1357:1364	arg1	important					1328:1336	important	1328:1336	important	1328:1336	Low-load compression testing, followed by three-element Maxwell analyses, showed that the fastest relaxation component, associated with unbound water, was most important in LB-medium-grown biofilms.
30745390	6	40	from	biofilms	1357:1364	arg1	component					1277:1285	the fastest relaxation component	1254:1285	the fastest relaxation component	1254:1285	Low-load compression testing, followed by three-element Maxwell analyses, showed that the fastest relaxation component, associated with unbound water, was most important in LB-medium-grown biofilms.
30745390	5	41	theme	100-μm-thick	1059:1070	arg1	biofilms					1072:1079	100-μm-thick biofilms	1059:1079	100-μm-thick biofilms that differed in their amounts of matrix environmental DNA (eDNA) and polysaccharides	1059:1165	P. aeruginosa biofilms were grown for 18 h in a constant-depth film fermenter, with mucin-containing artificial sputum medium (ASM+), artificial sputum medium without mucin (ASM-), or Luria-Bertani (LB) broth; this yielded 100-μm-thick biofilms that differed in their amounts of matrix environmental DNA (eDNA) and polysaccharides.
30745390	4	42	theme	biofilms	615:622	arg1	related					633:639	related	633:639	related	633:639	Since the viscoelasticity of biofilms has been related to antimicrobial penetration but not yet bacterial killing, this study aims to relate killing of P. aeruginosa, in its biofilm mode of growth, by three antimicrobials to biofilm viscoelasticity.
30745390	4	42	theme	biofilms	615:622	arg1	viscoelasticity					596:610	the viscoelasticity	592:610	the viscoelasticity of biofilms	592:622	Since the viscoelasticity of biofilms has been related to antimicrobial penetration but not yet bacterial killing, this study aims to relate killing of P. aeruginosa, in its biofilm mode of growth, by three antimicrobials to biofilm viscoelasticity.
30745390	3	43	theme	biofilm	557:563	arg1	components					574:583	different biofilm (matrix) components	547:583	different biofilm (matrix) components	547:583	These time constants reflect different biofilm (matrix) components.
30745390	9	44	theme	antimicrobial	1688:1700	arg1	peptide					1702:1708	an antimicrobial peptide	1685:1708	an antimicrobial peptide	1685:1708	P. aeruginosa in LB-medium-grown biofilms was killed most by exposure to tobramycin, colistin, or an antimicrobial peptide, while ASM+ provided the most protective matrix, with less water and most insoluble polysaccharides and eDNA.
30745390	4	45	theme	bacterial	682:690	arg1	killing					692:698	bacterial killing	682:698	bacterial killing	682:698	Since the viscoelasticity of biofilms has been related to antimicrobial penetration but not yet bacterial killing, this study aims to relate killing of P. aeruginosa, in its biofilm mode of growth, by three antimicrobials to biofilm viscoelasticity.
30745390	5	46	theme	mucin-containing	920:935	arg1	ASM+					963:966	ASM+	963:966	ASM+	963:966	P. aeruginosa biofilms were grown for 18 h in a constant-depth film fermenter, with mucin-containing artificial sputum medium (ASM+), artificial sputum medium without mucin (ASM-), or Luria-Bertani (LB) broth; this yielded 100-μm-thick biofilms that differed in their amounts of matrix environmental DNA (eDNA) and polysaccharides.
30745390	5	46	theme	mucin-containing	920:935	arg1	medium					955:960	mucin-containing artificial sputum medium	920:960	mucin-containing artificial sputum medium (ASM+)	920:967	P. aeruginosa biofilms were grown for 18 h in a constant-depth film fermenter, with mucin-containing artificial sputum medium (ASM+), artificial sputum medium without mucin (ASM-), or Luria-Bertani (LB) broth; this yielded 100-μm-thick biofilms that differed in their amounts of matrix environmental DNA (eDNA) and polysaccharides.
30745390	10	47	from	differences	1913:1923	arg1	composition					1935:1945	matrix composition	1928:1945	matrix composition	1928:1945	In conclusion, stress relaxation of P. aeruginosa biofilms grown in different media revealed differences in matrix composition that, within the constraints of the antimicrobials and growth media applied, correlated with the matrix protection offered against different antimicrobials.
30745390	1	48	theme	extracellular	296:308	arg1	matrices					336:343	their self-produced extracellular polymeric substance (EPS) matrices	276:343	their self-produced extracellular polymeric substance (EPS) matrices	276:343	Pseudomonas aeruginosa colonizes the sputum of most adult cystic fibrosis patients, forming difficult-to-eradicate biofilms in which bacteria are protected in their self-produced extracellular polymeric substance (EPS) matrices.
30745390	7	49	theme	ASM+-grown	1507:1516	arg1	biofilms					1518:1525	ASM+-grown biofilms	1507:1525	ASM+-grown biofilms	1507:1525	Slower components due to water with dissolved polysaccharides, insoluble polysaccharides, and eDNA were most important in the relaxation of ASM+-grown biofilms.
30745390	6	50	theme	three-element	1210:1222	arg1	analyses					1232:1239	three-element Maxwell analyses	1210:1239	three-element Maxwell analyses	1210:1239	Low-load compression testing, followed by three-element Maxwell analyses, showed that the fastest relaxation component, associated with unbound water, was most important in LB-medium-grown biofilms.
30745390	10	51	theme	media	2009:2013	arg1	constraints					1964:1974	the constraints	1960:1974	the constraints of the antimicrobials and growth media applied	1960:2021	In conclusion, stress relaxation of P. aeruginosa biofilms grown in different media revealed differences in matrix composition that, within the constraints of the antimicrobials and growth media applied, correlated with the matrix protection offered against different antimicrobials.
30745390	6	52	theme	unbound	1304:1310	arg1	water					1312:1316	unbound water	1304:1316	unbound water	1304:1316	Low-load compression testing, followed by three-element Maxwell analyses, showed that the fastest relaxation component, associated with unbound water, was most important in LB-medium-grown biofilms.
30745390	1	53	theme	fibrosis	182:189	arg1	patients					191:198	most adult cystic fibrosis patients	164:198	most adult cystic fibrosis patients	164:198	Pseudomonas aeruginosa colonizes the sputum of most adult cystic fibrosis patients, forming difficult-to-eradicate biofilms in which bacteria are protected in their self-produced extracellular polymeric substance (EPS) matrices.
30745390	9	54	theme	protective	1740:1749	arg1	matrix					1751:1756	the most protective matrix	1731:1756	the most protective matrix	1731:1756	P. aeruginosa in LB-medium-grown biofilms was killed most by exposure to tobramycin, colistin, or an antimicrobial peptide, while ASM+ provided the most protective matrix, with less water and most insoluble polysaccharides and eDNA.
30745390	5	55	theme	artificial	937:946	arg1	ASM+					963:966	ASM+	963:966	ASM+	963:966	P. aeruginosa biofilms were grown for 18 h in a constant-depth film fermenter, with mucin-containing artificial sputum medium (ASM+), artificial sputum medium without mucin (ASM-), or Luria-Bertani (LB) broth; this yielded 100-μm-thick biofilms that differed in their amounts of matrix environmental DNA (eDNA) and polysaccharides.
30745390	5	55	theme	artificial	937:946	arg1	medium					955:960	mucin-containing artificial sputum medium	920:960	mucin-containing artificial sputum medium (ASM+)	920:967	P. aeruginosa biofilms were grown for 18 h in a constant-depth film fermenter, with mucin-containing artificial sputum medium (ASM+), artificial sputum medium without mucin (ASM-), or Luria-Bertani (LB) broth; this yielded 100-μm-thick biofilms that differed in their amounts of matrix environmental DNA (eDNA) and polysaccharides.
30745390	10	56	theme	stress	1835:1840	arg1	relaxation					1842:1851	stress relaxation	1835:1851	stress relaxation of P. aeruginosa biofilms grown in different media	1835:1902	In conclusion, stress relaxation of P. aeruginosa biofilms grown in different media revealed differences in matrix composition that, within the constraints of the antimicrobials and growth media applied, correlated with the matrix protection offered against different antimicrobials.
30745390	5	57	theme	constant-depth	884:897	arg1	fermenter					904:912	a constant-depth film fermenter	882:912	a constant-depth film fermenter	882:912	P. aeruginosa biofilms were grown for 18 h in a constant-depth film fermenter, with mucin-containing artificial sputum medium (ASM+), artificial sputum medium without mucin (ASM-), or Luria-Bertani (LB) broth; this yielded 100-μm-thick biofilms that differed in their amounts of matrix environmental DNA (eDNA) and polysaccharides.
30745390	6	58	theme	Low-load	1168:1175	arg1	testing					1189:1195	Low-load compression testing	1168:1195	Low-load compression testing	1168:1195	Low-load compression testing, followed by three-element Maxwell analyses, showed that the fastest relaxation component, associated with unbound water, was most important in LB-medium-grown biofilms.
30745390	9	59	theme	LB-medium-grown	1604:1618	arg1	biofilms					1620:1627	LB-medium-grown biofilms	1604:1627	LB-medium-grown biofilms	1604:1627	P. aeruginosa in LB-medium-grown biofilms was killed most by exposure to tobramycin, colistin, or an antimicrobial peptide, while ASM+ provided the most protective matrix, with less water and most insoluble polysaccharides and eDNA.
30745390	6	60	theme	relaxation	1266:1275	arg1	important					1328:1336	important	1328:1336	important	1328:1336	Low-load compression testing, followed by three-element Maxwell analyses, showed that the fastest relaxation component, associated with unbound water, was most important in LB-medium-grown biofilms.
30745390	6	60	theme	relaxation	1266:1275	arg1	component					1277:1285	the fastest relaxation component	1254:1285	the fastest relaxation component	1254:1285	Low-load compression testing, followed by three-element Maxwell analyses, showed that the fastest relaxation component, associated with unbound water, was most important in LB-medium-grown biofilms.
30745390	5	61	theme	matrix	1115:1120	arg1	eDNA					1141:1144	eDNA	1141:1144	eDNA	1141:1144	P. aeruginosa biofilms were grown for 18 h in a constant-depth film fermenter, with mucin-containing artificial sputum medium (ASM+), artificial sputum medium without mucin (ASM-), or Luria-Bertani (LB) broth; this yielded 100-μm-thick biofilms that differed in their amounts of matrix environmental DNA (eDNA) and polysaccharides.
30745390	5	61	theme	matrix	1115:1120	arg1	DNA					1136:1138	matrix environmental DNA	1115:1138	matrix environmental DNA (eDNA)	1115:1145	P. aeruginosa biofilms were grown for 18 h in a constant-depth film fermenter, with mucin-containing artificial sputum medium (ASM+), artificial sputum medium without mucin (ASM-), or Luria-Bertani (LB) broth; this yielded 100-μm-thick biofilms that differed in their amounts of matrix environmental DNA (eDNA) and polysaccharides.
30745390	10	62	theme	aeruginosa	1859:1868	arg1	biofilms					1870:1877	P. aeruginosa biofilms	1856:1877	P. aeruginosa biofilms grown in different media	1856:1902	In conclusion, stress relaxation of P. aeruginosa biofilms grown in different media revealed differences in matrix composition that, within the constraints of the antimicrobials and growth media applied, correlated with the matrix protection offered against different antimicrobials.
30745390	9	63	theme	insoluble	1784:1792	arg1	polysaccharides					1794:1808	most insoluble polysaccharides	1779:1808	most insoluble polysaccharides	1779:1808	P. aeruginosa in LB-medium-grown biofilms was killed most by exposure to tobramycin, colistin, or an antimicrobial peptide, while ASM+ provided the most protective matrix, with less water and most insoluble polysaccharides and eDNA.
30745390	8	64	theme	ASM--grown	1528:1537	arg1	biofilms					1539:1546	ASM--grown biofilms	1528:1546	ASM--grown biofilms	1528:1546	ASM--grown biofilms showed intermediate stress relaxation.
30745390	10	65	theme	matrix	2044:2049	arg1	protection					2051:2060	the matrix protection	2040:2060	the matrix protection offered against different antimicrobials	2040:2101	In conclusion, stress relaxation of P. aeruginosa biofilms grown in different media revealed differences in matrix composition that, within the constraints of the antimicrobials and growth media applied, correlated with the matrix protection offered against different antimicrobials.
30745390	5	66	theme	sputum	981:986	arg1	medium					988:993	artificial sputum medium	970:993	artificial sputum medium without mucin (ASM-)	970:1014	P. aeruginosa biofilms were grown for 18 h in a constant-depth film fermenter, with mucin-containing artificial sputum medium (ASM+), artificial sputum medium without mucin (ASM-), or Luria-Bertani (LB) broth; this yielded 100-μm-thick biofilms that differed in their amounts of matrix environmental DNA (eDNA) and polysaccharides.
30745390	7	67	theme	dissolved	1403:1411	arg1	polysaccharides					1413:1427	dissolved polysaccharides	1403:1427	dissolved polysaccharides	1403:1427	Slower components due to water with dissolved polysaccharides, insoluble polysaccharides, and eDNA were most important in the relaxation of ASM+-grown biofilms.
30745390	4	68	theme	growth	776:781	arg1	mode					768:771	its biofilm mode	756:771	its biofilm mode of growth	756:781	Since the viscoelasticity of biofilms has been related to antimicrobial penetration but not yet bacterial killing, this study aims to relate killing of P. aeruginosa, in its biofilm mode of growth, by three antimicrobials to biofilm viscoelasticity.
30745390	10	69	theme	different	1888:1896	arg1	media					1898:1902	different media	1888:1902	different media	1888:1902	In conclusion, stress relaxation of P. aeruginosa biofilms grown in different media revealed differences in matrix composition that, within the constraints of the antimicrobials and growth media applied, correlated with the matrix protection offered against different antimicrobials.
30745390	1	70	theme	difficult-to-eradicate	209:230	arg1	biofilms					232:239	difficult-to-eradicate biofilms	209:239	difficult-to-eradicate biofilms in which bacteria are protected in their self-produced extracellular polymeric substance (EPS) matrices	209:343	Pseudomonas aeruginosa colonizes the sputum of most adult cystic fibrosis patients, forming difficult-to-eradicate biofilms in which bacteria are protected in their self-produced extracellular polymeric substance (EPS) matrices.
30745390	8	71	theme	stress	1568:1573	arg1	relaxation					1575:1584	intermediate stress relaxation	1555:1584	intermediate stress relaxation	1555:1584	ASM--grown biofilms showed intermediate stress relaxation.
30745390	10	72	theme	different	2078:2086	arg1	antimicrobials					2088:2101	different antimicrobials	2078:2101	different antimicrobials	2078:2101	In conclusion, stress relaxation of P. aeruginosa biofilms grown in different media revealed differences in matrix composition that, within the constraints of the antimicrobials and growth media applied, correlated with the matrix protection offered against different antimicrobials.
30745390	1	73	theme	adult	169:173	arg1	patients					191:198	most adult cystic fibrosis patients	164:198	most adult cystic fibrosis patients	164:198	Pseudomonas aeruginosa colonizes the sputum of most adult cystic fibrosis patients, forming difficult-to-eradicate biofilms in which bacteria are protected in their self-produced extracellular polymeric substance (EPS) matrices.
30745390	7	74	from	important	1476:1484	arg1	relaxation					1493:1502	the relaxation	1489:1502	the relaxation of ASM+-grown biofilms	1489:1525	Slower components due to water with dissolved polysaccharides, insoluble polysaccharides, and eDNA were most important in the relaxation of ASM+-grown biofilms.
30745390	4	75	theme	biofilm	811:817	arg1	viscoelasticity					819:833	biofilm viscoelasticity	811:833	biofilm viscoelasticity	811:833	Since the viscoelasticity of biofilms has been related to antimicrobial penetration but not yet bacterial killing, this study aims to relate killing of P. aeruginosa, in its biofilm mode of growth, by three antimicrobials to biofilm viscoelasticity.
30745390	5	76	theme	DNA	1136:1138	arg1	amounts					1104:1110	their amounts	1098:1110	their amounts of matrix environmental DNA (eDNA) and polysaccharides	1098:1165	P. aeruginosa biofilms were grown for 18 h in a constant-depth film fermenter, with mucin-containing artificial sputum medium (ASM+), artificial sputum medium without mucin (ASM-), or Luria-Bertani (LB) broth; this yielded 100-μm-thick biofilms that differed in their amounts of matrix environmental DNA (eDNA) and polysaccharides.
30745390	5	76	theme	DNA	1136:1138	arg1	eDNA					1141:1144	eDNA	1141:1144	eDNA	1141:1144	P. aeruginosa biofilms were grown for 18 h in a constant-depth film fermenter, with mucin-containing artificial sputum medium (ASM+), artificial sputum medium without mucin (ASM-), or Luria-Bertani (LB) broth; this yielded 100-μm-thick biofilms that differed in their amounts of matrix environmental DNA (eDNA) and polysaccharides.
30745390	5	76	theme	DNA	1136:1138	arg1	polysaccharides					1151:1165	polysaccharides	1151:1165	polysaccharides	1151:1165	P. aeruginosa biofilms were grown for 18 h in a constant-depth film fermenter, with mucin-containing artificial sputum medium (ASM+), artificial sputum medium without mucin (ASM-), or Luria-Bertani (LB) broth; this yielded 100-μm-thick biofilms that differed in their amounts of matrix environmental DNA (eDNA) and polysaccharides.
30745390	5	76	theme	DNA	1136:1138	arg1	DNA					1136:1138	matrix environmental DNA	1115:1138	matrix environmental DNA (eDNA)	1115:1145	P. aeruginosa biofilms were grown for 18 h in a constant-depth film fermenter, with mucin-containing artificial sputum medium (ASM+), artificial sputum medium without mucin (ASM-), or Luria-Bertani (LB) broth; this yielded 100-μm-thick biofilms that differed in their amounts of matrix environmental DNA (eDNA) and polysaccharides.
30745390	0	77	from	Killing	37:43	arg1	Biofilms					107:114	Differently Grown Pseudomonas aeruginosa Biofilms	66:114	Differently Grown Pseudomonas aeruginosa Biofilms	66:114	Role of Viscoelasticity in Bacterial Killing by Antimicrobials in Differently Grown Pseudomonas aeruginosa Biofilms.
30745390	2	78	theme	time	502:505	arg1	constants					507:515	multiple characteristic time constants	478:515	multiple characteristic time constants	478:515	EPS provide biofilms with viscoelastic properties, causing time-dependent relaxation after stress-induced deformation, according to multiple characteristic time constants.
30745390	4	79	theme	antimicrobial	644:656	arg1	penetration					658:668	antimicrobial penetration	644:668	antimicrobial penetration	644:668	Since the viscoelasticity of biofilms has been related to antimicrobial penetration but not yet bacterial killing, this study aims to relate killing of P. aeruginosa, in its biofilm mode of growth, by three antimicrobials to biofilm viscoelasticity.
30745390	3	80	theme	different	547:555	arg1	components					574:583	different biofilm (matrix) components	547:583	different biofilm (matrix) components	547:583	These time constants reflect different biofilm (matrix) components.
30745390	5	81	theme	aeruginosa	839:848	arg1	biofilms					850:857	P. aeruginosa biofilms	836:857	P. aeruginosa biofilms	836:857	P. aeruginosa biofilms were grown for 18 h in a constant-depth film fermenter, with mucin-containing artificial sputum medium (ASM+), artificial sputum medium without mucin (ASM-), or Luria-Bertani (LB) broth; this yielded 100-μm-thick biofilms that differed in their amounts of matrix environmental DNA (eDNA) and polysaccharides.
30745390	2	82	with	biofilms	358:365	arg1	properties					385:394	viscoelastic properties	372:394	viscoelastic properties	372:394	EPS provide biofilms with viscoelastic properties, causing time-dependent relaxation after stress-induced deformation, according to multiple characteristic time constants.
30745390	6	83	from	important	1328:1336	arg1	biofilms					1357:1364	LB-medium-grown biofilms	1341:1364	LB-medium-grown biofilms	1341:1364	Low-load compression testing, followed by three-element Maxwell analyses, showed that the fastest relaxation component, associated with unbound water, was most important in LB-medium-grown biofilms.
30745390	10	84	dep	antimicrobials	1983:1996	arg1	the					1979:1981	the	1979:1981	the	1979:1981	In conclusion, stress relaxation of P. aeruginosa biofilms grown in different media revealed differences in matrix composition that, within the constraints of the antimicrobials and growth media applied, correlated with the matrix protection offered against different antimicrobials.
30745390	2	85	theme	viscoelastic	372:383	arg1	properties					385:394	viscoelastic properties	372:394	viscoelastic properties	372:394	EPS provide biofilms with viscoelastic properties, causing time-dependent relaxation after stress-induced deformation, according to multiple characteristic time constants.
30745390	10	86	theme	antimicrobials	1983:1996	arg1	constraints					1964:1974	the constraints	1960:1974	the constraints of the antimicrobials and growth media applied	1960:2021	In conclusion, stress relaxation of P. aeruginosa biofilms grown in different media revealed differences in matrix composition that, within the constraints of the antimicrobials and growth media applied, correlated with the matrix protection offered against different antimicrobials.
30745390	2	87	theme	time-dependent	405:418	arg1	relaxation					420:429	time-dependent relaxation	405:429	time-dependent relaxation	405:429	EPS provide biofilms with viscoelastic properties, causing time-dependent relaxation after stress-induced deformation, according to multiple characteristic time constants.
30172513	0	0	theme	endogenous	101:110	arg1	glycopeptides					121:133	endogenous N-linked glycopeptides	101:133	endogenous N-linked glycopeptides	101:133	Core-shell structured magnetic metal-organic framework composites for highly selective enrichment of endogenous N-linked glycopeptides and phosphopeptides.
30172513	0	1	link	N-linked	112:119	arg1	glycopeptides					121:133	endogenous N-linked glycopeptides	101:133	endogenous N-linked glycopeptides	101:133	Core-shell structured magnetic metal-organic framework composites for highly selective enrichment of endogenous N-linked glycopeptides and phosphopeptides.
30172513	1	2	theme	structured	181:190	arg1	composites					225:234	core-shell structured magnetic metal-organic framework composites	170:234	core-shell structured magnetic metal-organic framework composites denoted as Fe3O4 @MIL-100(Fe)	170:264	In this work, core-shell structured magnetic metal-organic framework composites denoted as Fe3O4 @MIL-100(Fe) were synthesized by means of a layer-by-layer assembly method selecting Fe as metal center and 1,3,5-benzenetricarboxylic acid as organic ligand.
30172513	1	2	theme	structured	181:190	arg1	center					350:355	metal center	344:355	metal center	344:355	In this work, core-shell structured magnetic metal-organic framework composites denoted as Fe3O4 @MIL-100(Fe) were synthesized by means of a layer-by-layer assembly method selecting Fe as metal center and 1,3,5-benzenetricarboxylic acid as organic ligand.
30172513	1	2	theme	structured	181:190	arg1	acid					388:391	1,3,5-benzenetricarboxylic acid	361:391	1,3,5-benzenetricarboxylic acid	361:391	In this work, core-shell structured magnetic metal-organic framework composites denoted as Fe3O4 @MIL-100(Fe) were synthesized by means of a layer-by-layer assembly method selecting Fe as metal center and 1,3,5-benzenetricarboxylic acid as organic ligand.
30172513	1	2	theme	structured	181:190	arg1	ligand					404:409	organic ligand	396:409	organic ligand	396:409	In this work, core-shell structured magnetic metal-organic framework composites denoted as Fe3O4 @MIL-100(Fe) were synthesized by means of a layer-by-layer assembly method selecting Fe as metal center and 1,3,5-benzenetricarboxylic acid as organic ligand.
30172513	3	3	theme	great	900:904	arg1	results					906:912	great results	900:912	great results (43 phosphopeptides and 39 N-linked glycopeptides)	900:963	Moreover, it was applied into the enrichment of endogenous N-linked glycopeptides and phosphopeptides in human saliva and achieved great results (43 phosphopeptides and 39 N-linked glycopeptides), revealing its promising potential in enrichment of low-abundance endogenous N-linked glycopeptides and phosphopeptides in practical samples.
30172513	3	4	theme	N-linked	828:835	arg1	glycopeptides					837:849	endogenous N-linked glycopeptides	817:849	endogenous N-linked glycopeptides	817:849	Moreover, it was applied into the enrichment of endogenous N-linked glycopeptides and phosphopeptides in human saliva and achieved great results (43 phosphopeptides and 39 N-linked glycopeptides), revealing its promising potential in enrichment of low-abundance endogenous N-linked glycopeptides and phosphopeptides in practical samples.
30172513	1	5	theme	1,3,5-benzenetricarboxylic	361:386	arg1	composites					225:234	core-shell structured magnetic metal-organic framework composites	170:234	core-shell structured magnetic metal-organic framework composites denoted as Fe3O4 @MIL-100(Fe)	170:264	In this work, core-shell structured magnetic metal-organic framework composites denoted as Fe3O4 @MIL-100(Fe) were synthesized by means of a layer-by-layer assembly method selecting Fe as metal center and 1,3,5-benzenetricarboxylic acid as organic ligand.
30172513	1	5	theme	1,3,5-benzenetricarboxylic	361:386	arg1	acid					388:391	1,3,5-benzenetricarboxylic acid	361:391	1,3,5-benzenetricarboxylic acid	361:391	In this work, core-shell structured magnetic metal-organic framework composites denoted as Fe3O4 @MIL-100(Fe) were synthesized by means of a layer-by-layer assembly method selecting Fe as metal center and 1,3,5-benzenetricarboxylic acid as organic ligand.
30172513	3	6	theme	endogenous	1031:1040	arg1	glycopeptides					1051:1063	low-abundance endogenous N-linked glycopeptides	1017:1063	low-abundance endogenous N-linked glycopeptides	1017:1063	Moreover, it was applied into the enrichment of endogenous N-linked glycopeptides and phosphopeptides in human saliva and achieved great results (43 phosphopeptides and 39 N-linked glycopeptides), revealing its promising potential in enrichment of low-abundance endogenous N-linked glycopeptides and phosphopeptides in practical samples.
30172513	1	7	theme	magnetic	192:199	arg1	composites					225:234	core-shell structured magnetic metal-organic framework composites	170:234	core-shell structured magnetic metal-organic framework composites denoted as Fe3O4 @MIL-100(Fe)	170:264	In this work, core-shell structured magnetic metal-organic framework composites denoted as Fe3O4 @MIL-100(Fe) were synthesized by means of a layer-by-layer assembly method selecting Fe as metal center and 1,3,5-benzenetricarboxylic acid as organic ligand.
30172513	1	7	theme	magnetic	192:199	arg1	center					350:355	metal center	344:355	metal center	344:355	In this work, core-shell structured magnetic metal-organic framework composites denoted as Fe3O4 @MIL-100(Fe) were synthesized by means of a layer-by-layer assembly method selecting Fe as metal center and 1,3,5-benzenetricarboxylic acid as organic ligand.
30172513	1	7	theme	magnetic	192:199	arg1	acid					388:391	1,3,5-benzenetricarboxylic acid	361:391	1,3,5-benzenetricarboxylic acid	361:391	In this work, core-shell structured magnetic metal-organic framework composites denoted as Fe3O4 @MIL-100(Fe) were synthesized by means of a layer-by-layer assembly method selecting Fe as metal center and 1,3,5-benzenetricarboxylic acid as organic ligand.
30172513	1	7	theme	magnetic	192:199	arg1	ligand					404:409	organic ligand	396:409	organic ligand	396:409	In this work, core-shell structured magnetic metal-organic framework composites denoted as Fe3O4 @MIL-100(Fe) were synthesized by means of a layer-by-layer assembly method selecting Fe as metal center and 1,3,5-benzenetricarboxylic acid as organic ligand.
30172513	3	8	gly	glycopeptides	837:849	arg2	glycopeptides					837:849	endogenous N-linked glycopeptides	817:849	endogenous N-linked glycopeptides	817:849	Moreover, it was applied into the enrichment of endogenous N-linked glycopeptides and phosphopeptides in human saliva and achieved great results (43 phosphopeptides and 39 N-linked glycopeptides), revealing its promising potential in enrichment of low-abundance endogenous N-linked glycopeptides and phosphopeptides in practical samples.
30172513	0	9	theme	glycopeptides	121:133	arg1	enrichment					87:96	highly selective enrichment	70:96	highly selective enrichment of endogenous N-linked glycopeptides and phosphopeptides	70:153	Core-shell structured magnetic metal-organic framework composites for highly selective enrichment of endogenous N-linked glycopeptides and phosphopeptides.
30172513	3	10	theme	practical	1088:1096	arg1	samples					1098:1104	practical samples	1088:1104	practical samples	1088:1104	Moreover, it was applied into the enrichment of endogenous N-linked glycopeptides and phosphopeptides in human saliva and achieved great results (43 phosphopeptides and 39 N-linked glycopeptides), revealing its promising potential in enrichment of low-abundance endogenous N-linked glycopeptides and phosphopeptides in practical samples.
30172513	3	11	theme	N-linked	1042:1049	arg1	glycopeptides					1051:1063	low-abundance endogenous N-linked glycopeptides	1017:1063	low-abundance endogenous N-linked glycopeptides	1017:1063	Moreover, it was applied into the enrichment of endogenous N-linked glycopeptides and phosphopeptides in human saliva and achieved great results (43 phosphopeptides and 39 N-linked glycopeptides), revealing its promising potential in enrichment of low-abundance endogenous N-linked glycopeptides and phosphopeptides in practical samples.
30172513	2	12	theme	standard	722:729	arg1	proteins					731:738	standard proteins	722:738	standard proteins	722:738	The as-prepared material exhibited outstanding sensitivity (0.1 fmol/μL), good selectivity (1:20 and 1:50 respectively), excellent ability of size-exclusion (1: 500), fine reusability (six cycles) and great stability (two months) in enriching N-linked glycopeptides and phosphopeptides from tryptic digests of standard proteins by combining HILIC and IMAC.
30172513	0	13	theme	N-linked	112:119	arg1	glycopeptides					121:133	endogenous N-linked glycopeptides	101:133	endogenous N-linked glycopeptides	101:133	Core-shell structured magnetic metal-organic framework composites for highly selective enrichment of endogenous N-linked glycopeptides and phosphopeptides.
30172513	3	14	link	N-linked	828:835	arg1	glycopeptides					837:849	endogenous N-linked glycopeptides	817:849	endogenous N-linked glycopeptides	817:849	Moreover, it was applied into the enrichment of endogenous N-linked glycopeptides and phosphopeptides in human saliva and achieved great results (43 phosphopeptides and 39 N-linked glycopeptides), revealing its promising potential in enrichment of low-abundance endogenous N-linked glycopeptides and phosphopeptides in practical samples.
30172513	3	15	link	N-linked	1042:1049	arg1	glycopeptides					1051:1063	low-abundance endogenous N-linked glycopeptides	1017:1063	low-abundance endogenous N-linked glycopeptides	1017:1063	Moreover, it was applied into the enrichment of endogenous N-linked glycopeptides and phosphopeptides in human saliva and achieved great results (43 phosphopeptides and 39 N-linked glycopeptides), revealing its promising potential in enrichment of low-abundance endogenous N-linked glycopeptides and phosphopeptides in practical samples.
30172513	0	16	theme	phosphopeptides	139:153	arg1	enrichment					87:96	highly selective enrichment	70:96	highly selective enrichment of endogenous N-linked glycopeptides and phosphopeptides	70:153	Core-shell structured magnetic metal-organic framework composites for highly selective enrichment of endogenous N-linked glycopeptides and phosphopeptides.
30172513	1	17	theme	organic	396:402	arg1	composites					225:234	core-shell structured magnetic metal-organic framework composites	170:234	core-shell structured magnetic metal-organic framework composites denoted as Fe3O4 @MIL-100(Fe)	170:264	In this work, core-shell structured magnetic metal-organic framework composites denoted as Fe3O4 @MIL-100(Fe) were synthesized by means of a layer-by-layer assembly method selecting Fe as metal center and 1,3,5-benzenetricarboxylic acid as organic ligand.
30172513	1	17	theme	organic	396:402	arg1	ligand					404:409	organic ligand	396:409	organic ligand	396:409	In this work, core-shell structured magnetic metal-organic framework composites denoted as Fe3O4 @MIL-100(Fe) were synthesized by means of a layer-by-layer assembly method selecting Fe as metal center and 1,3,5-benzenetricarboxylic acid as organic ligand.
30172513	3	18	from	potential	990:998	arg1	enrichment					1003:1012	enrichment	1003:1012	enrichment of low-abundance endogenous N-linked glycopeptides and phosphopeptides in practical samples	1003:1104	Moreover, it was applied into the enrichment of endogenous N-linked glycopeptides and phosphopeptides in human saliva and achieved great results (43 phosphopeptides and 39 N-linked glycopeptides), revealing its promising potential in enrichment of low-abundance endogenous N-linked glycopeptides and phosphopeptides in practical samples.
30172513	3	19	theme	low-abundance	1017:1029	arg1	glycopeptides					1051:1063	low-abundance endogenous N-linked glycopeptides	1017:1063	low-abundance endogenous N-linked glycopeptides	1017:1063	Moreover, it was applied into the enrichment of endogenous N-linked glycopeptides and phosphopeptides in human saliva and achieved great results (43 phosphopeptides and 39 N-linked glycopeptides), revealing its promising potential in enrichment of low-abundance endogenous N-linked glycopeptides and phosphopeptides in practical samples.
30172513	1	20	theme	metal-organic	201:213	arg1	composites					225:234	core-shell structured magnetic metal-organic framework composites	170:234	core-shell structured magnetic metal-organic framework composites denoted as Fe3O4 @MIL-100(Fe)	170:264	In this work, core-shell structured magnetic metal-organic framework composites denoted as Fe3O4 @MIL-100(Fe) were synthesized by means of a layer-by-layer assembly method selecting Fe as metal center and 1,3,5-benzenetricarboxylic acid as organic ligand.
30172513	1	20	theme	metal-organic	201:213	arg1	center					350:355	metal center	344:355	metal center	344:355	In this work, core-shell structured magnetic metal-organic framework composites denoted as Fe3O4 @MIL-100(Fe) were synthesized by means of a layer-by-layer assembly method selecting Fe as metal center and 1,3,5-benzenetricarboxylic acid as organic ligand.
30172513	1	20	theme	metal-organic	201:213	arg1	acid					388:391	1,3,5-benzenetricarboxylic acid	361:391	1,3,5-benzenetricarboxylic acid	361:391	In this work, core-shell structured magnetic metal-organic framework composites denoted as Fe3O4 @MIL-100(Fe) were synthesized by means of a layer-by-layer assembly method selecting Fe as metal center and 1,3,5-benzenetricarboxylic acid as organic ligand.
30172513	1	20	theme	metal-organic	201:213	arg1	ligand					404:409	organic ligand	396:409	organic ligand	396:409	In this work, core-shell structured magnetic metal-organic framework composites denoted as Fe3O4 @MIL-100(Fe) were synthesized by means of a layer-by-layer assembly method selecting Fe as metal center and 1,3,5-benzenetricarboxylic acid as organic ligand.
30172513	3	21	theme	endogenous	817:826	arg1	glycopeptides					837:849	endogenous N-linked glycopeptides	817:849	endogenous N-linked glycopeptides	817:849	Moreover, it was applied into the enrichment of endogenous N-linked glycopeptides and phosphopeptides in human saliva and achieved great results (43 phosphopeptides and 39 N-linked glycopeptides), revealing its promising potential in enrichment of low-abundance endogenous N-linked glycopeptides and phosphopeptides in practical samples.
30172513	3	22	theme	phosphopeptides	1069:1083	arg1	enrichment					1003:1012	enrichment	1003:1012	enrichment of low-abundance endogenous N-linked glycopeptides and phosphopeptides in practical samples	1003:1104	Moreover, it was applied into the enrichment of endogenous N-linked glycopeptides and phosphopeptides in human saliva and achieved great results (43 phosphopeptides and 39 N-linked glycopeptides), revealing its promising potential in enrichment of low-abundance endogenous N-linked glycopeptides and phosphopeptides in practical samples.
30172513	2	23	theme	good	486:489	arg1	selectivity					491:501	good selectivity	486:501	good selectivity (1:20 and 1:50 respectively)	486:530	The as-prepared material exhibited outstanding sensitivity (0.1 fmol/μL), good selectivity (1:20 and 1:50 respectively), excellent ability of size-exclusion (1: 500), fine reusability (six cycles) and great stability (two months) in enriching N-linked glycopeptides and phosphopeptides from tryptic digests of standard proteins by combining HILIC and IMAC.
30172513	1	24	theme	framework	215:223	arg1	composites					225:234	core-shell structured magnetic metal-organic framework composites	170:234	core-shell structured magnetic metal-organic framework composites denoted as Fe3O4 @MIL-100(Fe)	170:264	In this work, core-shell structured magnetic metal-organic framework composites denoted as Fe3O4 @MIL-100(Fe) were synthesized by means of a layer-by-layer assembly method selecting Fe as metal center and 1,3,5-benzenetricarboxylic acid as organic ligand.
30172513	1	24	theme	framework	215:223	arg1	center					350:355	metal center	344:355	metal center	344:355	In this work, core-shell structured magnetic metal-organic framework composites denoted as Fe3O4 @MIL-100(Fe) were synthesized by means of a layer-by-layer assembly method selecting Fe as metal center and 1,3,5-benzenetricarboxylic acid as organic ligand.
30172513	1	24	theme	framework	215:223	arg1	acid					388:391	1,3,5-benzenetricarboxylic acid	361:391	1,3,5-benzenetricarboxylic acid	361:391	In this work, core-shell structured magnetic metal-organic framework composites denoted as Fe3O4 @MIL-100(Fe) were synthesized by means of a layer-by-layer assembly method selecting Fe as metal center and 1,3,5-benzenetricarboxylic acid as organic ligand.
30172513	1	24	theme	framework	215:223	arg1	ligand					404:409	organic ligand	396:409	organic ligand	396:409	In this work, core-shell structured magnetic metal-organic framework composites denoted as Fe3O4 @MIL-100(Fe) were synthesized by means of a layer-by-layer assembly method selecting Fe as metal center and 1,3,5-benzenetricarboxylic acid as organic ligand.
30172513	0	25	theme	structured	11:20	arg1	framework					45:53	Core-shell structured magnetic metal-organic framework	0:53	Core-shell structured magnetic metal-organic framework	0:53	Core-shell structured magnetic metal-organic framework composites for highly selective enrichment of endogenous N-linked glycopeptides and phosphopeptides.
30172513	2	26	dep	selectivity	491:501	arg1	respectively					518:529	respectively	518:529	respectively	518:529	The as-prepared material exhibited outstanding sensitivity (0.1 fmol/μL), good selectivity (1:20 and 1:50 respectively), excellent ability of size-exclusion (1: 500), fine reusability (six cycles) and great stability (two months) in enriching N-linked glycopeptides and phosphopeptides from tryptic digests of standard proteins by combining HILIC and IMAC.
30172513	2	27	theme	as-prepared	416:426	arg1	material					428:435	The as-prepared material	412:435	The as-prepared material	412:435	The as-prepared material exhibited outstanding sensitivity (0.1 fmol/μL), good selectivity (1:20 and 1:50 respectively), excellent ability of size-exclusion (1: 500), fine reusability (six cycles) and great stability (two months) in enriching N-linked glycopeptides and phosphopeptides from tryptic digests of standard proteins by combining HILIC and IMAC.
30172513	0	28	theme	Core-shell	0:9	arg1	framework					45:53	Core-shell structured magnetic metal-organic framework	0:53	Core-shell structured magnetic metal-organic framework	0:53	Core-shell structured magnetic metal-organic framework composites for highly selective enrichment of endogenous N-linked glycopeptides and phosphopeptides.
30172513	2	29	theme	great	613:617	arg1	stability					619:627	great stability	613:627	great stability (two months)	613:640	The as-prepared material exhibited outstanding sensitivity (0.1 fmol/μL), good selectivity (1:20 and 1:50 respectively), excellent ability of size-exclusion (1: 500), fine reusability (six cycles) and great stability (two months) in enriching N-linked glycopeptides and phosphopeptides from tryptic digests of standard proteins by combining HILIC and IMAC.
30172513	2	29	theme	great	613:617	arg1	months					634:639	two months	630:639	two months	630:639	The as-prepared material exhibited outstanding sensitivity (0.1 fmol/μL), good selectivity (1:20 and 1:50 respectively), excellent ability of size-exclusion (1: 500), fine reusability (six cycles) and great stability (two months) in enriching N-linked glycopeptides and phosphopeptides from tryptic digests of standard proteins by combining HILIC and IMAC.
30172513	3	30	theme	glycopeptides	1051:1063	arg1	enrichment					1003:1012	enrichment	1003:1012	enrichment of low-abundance endogenous N-linked glycopeptides and phosphopeptides in practical samples	1003:1104	Moreover, it was applied into the enrichment of endogenous N-linked glycopeptides and phosphopeptides in human saliva and achieved great results (43 phosphopeptides and 39 N-linked glycopeptides), revealing its promising potential in enrichment of low-abundance endogenous N-linked glycopeptides and phosphopeptides in practical samples.
30172513	0	31	gly	glycopeptides	121:133	arg2	glycopeptides					121:133	endogenous N-linked glycopeptides	101:133	endogenous N-linked glycopeptides	101:133	Core-shell structured magnetic metal-organic framework composites for highly selective enrichment of endogenous N-linked glycopeptides and phosphopeptides.
30172513	3	32	theme	N-linked	941:948	arg1	glycopeptides					950:962	39 N-linked glycopeptides	938:962	39 N-linked glycopeptides	938:962	Moreover, it was applied into the enrichment of endogenous N-linked glycopeptides and phosphopeptides in human saliva and achieved great results (43 phosphopeptides and 39 N-linked glycopeptides), revealing its promising potential in enrichment of low-abundance endogenous N-linked glycopeptides and phosphopeptides in practical samples.
30172513	2	33	from	digests	711:717	arg1	glycopeptides					664:676	N-linked glycopeptides	655:676	N-linked glycopeptides	655:676	The as-prepared material exhibited outstanding sensitivity (0.1 fmol/μL), good selectivity (1:20 and 1:50 respectively), excellent ability of size-exclusion (1: 500), fine reusability (six cycles) and great stability (two months) in enriching N-linked glycopeptides and phosphopeptides from tryptic digests of standard proteins by combining HILIC and IMAC.
30172513	2	33	from	digests	711:717	arg1	phosphopeptides					682:696	phosphopeptides	682:696	phosphopeptides	682:696	The as-prepared material exhibited outstanding sensitivity (0.1 fmol/μL), good selectivity (1:20 and 1:50 respectively), excellent ability of size-exclusion (1: 500), fine reusability (six cycles) and great stability (two months) in enriching N-linked glycopeptides and phosphopeptides from tryptic digests of standard proteins by combining HILIC and IMAC.
30172513	0	34	theme	metal-organic	31:43	arg1	framework					45:53	Core-shell structured magnetic metal-organic framework	0:53	Core-shell structured magnetic metal-organic framework	0:53	Core-shell structured magnetic metal-organic framework composites for highly selective enrichment of endogenous N-linked glycopeptides and phosphopeptides.
30172513	1	35	theme	layer-by-layer	297:310	arg1	method					321:326	a layer-by-layer assembly method	295:326	a layer-by-layer assembly method selecting Fe	295:339	In this work, core-shell structured magnetic metal-organic framework composites denoted as Fe3O4 @MIL-100(Fe) were synthesized by means of a layer-by-layer assembly method selecting Fe as metal center and 1,3,5-benzenetricarboxylic acid as organic ligand.
30172513	2	36	theme	N-linked	655:662	arg1	glycopeptides					664:676	N-linked glycopeptides	655:676	N-linked glycopeptides	655:676	The as-prepared material exhibited outstanding sensitivity (0.1 fmol/μL), good selectivity (1:20 and 1:50 respectively), excellent ability of size-exclusion (1: 500), fine reusability (six cycles) and great stability (two months) in enriching N-linked glycopeptides and phosphopeptides from tryptic digests of standard proteins by combining HILIC and IMAC.
30172513	0	37	theme	magnetic	22:29	arg1	framework					45:53	Core-shell structured magnetic metal-organic framework	0:53	Core-shell structured magnetic metal-organic framework	0:53	Core-shell structured magnetic metal-organic framework composites for highly selective enrichment of endogenous N-linked glycopeptides and phosphopeptides.
30172513	1	38	theme	assembly	312:319	arg1	method					321:326	a layer-by-layer assembly method	295:326	a layer-by-layer assembly method selecting Fe	295:339	In this work, core-shell structured magnetic metal-organic framework composites denoted as Fe3O4 @MIL-100(Fe) were synthesized by means of a layer-by-layer assembly method selecting Fe as metal center and 1,3,5-benzenetricarboxylic acid as organic ligand.
30172513	3	39	link	N-linked	941:948	arg1	glycopeptides					950:962	39 N-linked glycopeptides	938:962	39 N-linked glycopeptides	938:962	Moreover, it was applied into the enrichment of endogenous N-linked glycopeptides and phosphopeptides in human saliva and achieved great results (43 phosphopeptides and 39 N-linked glycopeptides), revealing its promising potential in enrichment of low-abundance endogenous N-linked glycopeptides and phosphopeptides in practical samples.
30172513	1	40	theme	Fe3O4	247:251	arg1	Fe					262:263	Fe	262:263	Fe	262:263	In this work, core-shell structured magnetic metal-organic framework composites denoted as Fe3O4 @MIL-100(Fe) were synthesized by means of a layer-by-layer assembly method selecting Fe as metal center and 1,3,5-benzenetricarboxylic acid as organic ligand.
30172513	1	40	theme	Fe3O4	247:251	arg1	MIL-100					254:260	Fe3O4 @MIL-100	247:260	Fe3O4 @MIL-100(Fe)	247:264	In this work, core-shell structured magnetic metal-organic framework composites denoted as Fe3O4 @MIL-100(Fe) were synthesized by means of a layer-by-layer assembly method selecting Fe as metal center and 1,3,5-benzenetricarboxylic acid as organic ligand.
30172513	3	41	theme	human	874:878	arg1	saliva					880:885	human saliva	874:885	human saliva	874:885	Moreover, it was applied into the enrichment of endogenous N-linked glycopeptides and phosphopeptides in human saliva and achieved great results (43 phosphopeptides and 39 N-linked glycopeptides), revealing its promising potential in enrichment of low-abundance endogenous N-linked glycopeptides and phosphopeptides in practical samples.
30172513	3	42	theme	phosphopeptides	855:869	arg1	enrichment					803:812	the enrichment	799:812	the enrichment of endogenous N-linked glycopeptides and phosphopeptides in human saliva	799:885	Moreover, it was applied into the enrichment of endogenous N-linked glycopeptides and phosphopeptides in human saliva and achieved great results (43 phosphopeptides and 39 N-linked glycopeptides), revealing its promising potential in enrichment of low-abundance endogenous N-linked glycopeptides and phosphopeptides in practical samples.
30172513	1	43	theme	@	253:253	arg1	Fe					262:263	Fe	262:263	Fe	262:263	In this work, core-shell structured magnetic metal-organic framework composites denoted as Fe3O4 @MIL-100(Fe) were synthesized by means of a layer-by-layer assembly method selecting Fe as metal center and 1,3,5-benzenetricarboxylic acid as organic ligand.
30172513	1	43	theme	@	253:253	arg1	MIL-100					254:260	Fe3O4 @MIL-100	247:260	Fe3O4 @MIL-100(Fe)	247:264	In this work, core-shell structured magnetic metal-organic framework composites denoted as Fe3O4 @MIL-100(Fe) were synthesized by means of a layer-by-layer assembly method selecting Fe as metal center and 1,3,5-benzenetricarboxylic acid as organic ligand.
30172513	2	44	link	N-linked	655:662	arg1	glycopeptides					664:676	N-linked glycopeptides	655:676	N-linked glycopeptides	655:676	The as-prepared material exhibited outstanding sensitivity (0.1 fmol/μL), good selectivity (1:20 and 1:50 respectively), excellent ability of size-exclusion (1: 500), fine reusability (six cycles) and great stability (two months) in enriching N-linked glycopeptides and phosphopeptides from tryptic digests of standard proteins by combining HILIC and IMAC.
30172513	3	45	theme	glycopeptides	837:849	arg1	enrichment					803:812	the enrichment	799:812	the enrichment of endogenous N-linked glycopeptides and phosphopeptides in human saliva	799:885	Moreover, it was applied into the enrichment of endogenous N-linked glycopeptides and phosphopeptides in human saliva and achieved great results (43 phosphopeptides and 39 N-linked glycopeptides), revealing its promising potential in enrichment of low-abundance endogenous N-linked glycopeptides and phosphopeptides in practical samples.
30172513	3	46	gly	glycopeptides	950:962	arg2	glycopeptides					950:962	39 N-linked glycopeptides	938:962	39 N-linked glycopeptides	938:962	Moreover, it was applied into the enrichment of endogenous N-linked glycopeptides and phosphopeptides in human saliva and achieved great results (43 phosphopeptides and 39 N-linked glycopeptides), revealing its promising potential in enrichment of low-abundance endogenous N-linked glycopeptides and phosphopeptides in practical samples.
30172513	2	47	theme	outstanding	447:457	arg1	sensitivity					459:469	outstanding sensitivity	447:469	outstanding sensitivity (0.1 fmol/μL)	447:483	The as-prepared material exhibited outstanding sensitivity (0.1 fmol/μL), good selectivity (1:20 and 1:50 respectively), excellent ability of size-exclusion (1: 500), fine reusability (six cycles) and great stability (two months) in enriching N-linked glycopeptides and phosphopeptides from tryptic digests of standard proteins by combining HILIC and IMAC.
30172513	2	47	theme	outstanding	447:457	arg1	0.1 fmol/μL					472:482	0.1 fmol/μL	472:482	0.1 fmol/μL	472:482	The as-prepared material exhibited outstanding sensitivity (0.1 fmol/μL), good selectivity (1:20 and 1:50 respectively), excellent ability of size-exclusion (1: 500), fine reusability (six cycles) and great stability (two months) in enriching N-linked glycopeptides and phosphopeptides from tryptic digests of standard proteins by combining HILIC and IMAC.
30172513	2	48	theme	size-exclusion	554:567	arg1	months					634:639	two months	630:639	two months	630:639	The as-prepared material exhibited outstanding sensitivity (0.1 fmol/μL), good selectivity (1:20 and 1:50 respectively), excellent ability of size-exclusion (1: 500), fine reusability (six cycles) and great stability (two months) in enriching N-linked glycopeptides and phosphopeptides from tryptic digests of standard proteins by combining HILIC and IMAC.
30172513	2	48	theme	size-exclusion	554:567	arg1	stability					619:627	great stability	613:627	great stability (two months)	613:640	The as-prepared material exhibited outstanding sensitivity (0.1 fmol/μL), good selectivity (1:20 and 1:50 respectively), excellent ability of size-exclusion (1: 500), fine reusability (six cycles) and great stability (two months) in enriching N-linked glycopeptides and phosphopeptides from tryptic digests of standard proteins by combining HILIC and IMAC.
30172513	2	48	theme	size-exclusion	554:567	arg1	selectivity					491:501	good selectivity	486:501	good selectivity (1:20 and 1:50 respectively)	486:530	The as-prepared material exhibited outstanding sensitivity (0.1 fmol/μL), good selectivity (1:20 and 1:50 respectively), excellent ability of size-exclusion (1: 500), fine reusability (six cycles) and great stability (two months) in enriching N-linked glycopeptides and phosphopeptides from tryptic digests of standard proteins by combining HILIC and IMAC.
30172513	2	48	theme	size-exclusion	554:567	arg1	reusability					584:594	fine reusability	579:594	fine reusability (six cycles)	579:607	The as-prepared material exhibited outstanding sensitivity (0.1 fmol/μL), good selectivity (1:20 and 1:50 respectively), excellent ability of size-exclusion (1: 500), fine reusability (six cycles) and great stability (two months) in enriching N-linked glycopeptides and phosphopeptides from tryptic digests of standard proteins by combining HILIC and IMAC.
30172513	2	48	theme	size-exclusion	554:567	arg1	sensitivity					459:469	outstanding sensitivity	447:469	outstanding sensitivity (0.1 fmol/μL)	447:483	The as-prepared material exhibited outstanding sensitivity (0.1 fmol/μL), good selectivity (1:20 and 1:50 respectively), excellent ability of size-exclusion (1: 500), fine reusability (six cycles) and great stability (two months) in enriching N-linked glycopeptides and phosphopeptides from tryptic digests of standard proteins by combining HILIC and IMAC.
30172513	2	48	theme	size-exclusion	554:567	arg1	0.1 fmol/μL					472:482	0.1 fmol/μL	472:482	0.1 fmol/μL	472:482	The as-prepared material exhibited outstanding sensitivity (0.1 fmol/μL), good selectivity (1:20 and 1:50 respectively), excellent ability of size-exclusion (1: 500), fine reusability (six cycles) and great stability (two months) in enriching N-linked glycopeptides and phosphopeptides from tryptic digests of standard proteins by combining HILIC and IMAC.
30172513	2	48	theme	size-exclusion	554:567	arg1	cycles					601:606	six cycles	597:606	six cycles	597:606	The as-prepared material exhibited outstanding sensitivity (0.1 fmol/μL), good selectivity (1:20 and 1:50 respectively), excellent ability of size-exclusion (1: 500), fine reusability (six cycles) and great stability (two months) in enriching N-linked glycopeptides and phosphopeptides from tryptic digests of standard proteins by combining HILIC and IMAC.
30172513	2	48	theme	size-exclusion	554:567	arg1	ability					543:549	excellent ability	533:549	excellent ability of size-exclusion (1: 500)	533:576	The as-prepared material exhibited outstanding sensitivity (0.1 fmol/μL), good selectivity (1:20 and 1:50 respectively), excellent ability of size-exclusion (1: 500), fine reusability (six cycles) and great stability (two months) in enriching N-linked glycopeptides and phosphopeptides from tryptic digests of standard proteins by combining HILIC and IMAC.
30172513	3	49	from	enrichment	1003:1012	arg1	samples					1098:1104	practical samples	1088:1104	practical samples	1088:1104	Moreover, it was applied into the enrichment of endogenous N-linked glycopeptides and phosphopeptides in human saliva and achieved great results (43 phosphopeptides and 39 N-linked glycopeptides), revealing its promising potential in enrichment of low-abundance endogenous N-linked glycopeptides and phosphopeptides in practical samples.
30172513	2	50	dep	respectively	518:529	arg1	1:20					504:507	1:20	504:507	1:20	504:507	The as-prepared material exhibited outstanding sensitivity (0.1 fmol/μL), good selectivity (1:20 and 1:50 respectively), excellent ability of size-exclusion (1: 500), fine reusability (six cycles) and great stability (two months) in enriching N-linked glycopeptides and phosphopeptides from tryptic digests of standard proteins by combining HILIC and IMAC.
30172513	2	50	dep	respectively	518:529	arg1	1:50					513:516	1:50	513:516	1:50	513:516	The as-prepared material exhibited outstanding sensitivity (0.1 fmol/μL), good selectivity (1:20 and 1:50 respectively), excellent ability of size-exclusion (1: 500), fine reusability (six cycles) and great stability (two months) in enriching N-linked glycopeptides and phosphopeptides from tryptic digests of standard proteins by combining HILIC and IMAC.
30172513	2	51	theme	tryptic	703:709	arg1	digests					711:717	tryptic digests	703:717	tryptic digests of standard proteins	703:738	The as-prepared material exhibited outstanding sensitivity (0.1 fmol/μL), good selectivity (1:20 and 1:50 respectively), excellent ability of size-exclusion (1: 500), fine reusability (six cycles) and great stability (two months) in enriching N-linked glycopeptides and phosphopeptides from tryptic digests of standard proteins by combining HILIC and IMAC.
30172513	1	52	theme	metal	344:348	arg1	composites					225:234	core-shell structured magnetic metal-organic framework composites	170:234	core-shell structured magnetic metal-organic framework composites denoted as Fe3O4 @MIL-100(Fe)	170:264	In this work, core-shell structured magnetic metal-organic framework composites denoted as Fe3O4 @MIL-100(Fe) were synthesized by means of a layer-by-layer assembly method selecting Fe as metal center and 1,3,5-benzenetricarboxylic acid as organic ligand.
30172513	1	52	theme	metal	344:348	arg1	center					350:355	metal center	344:355	metal center	344:355	In this work, core-shell structured magnetic metal-organic framework composites denoted as Fe3O4 @MIL-100(Fe) were synthesized by means of a layer-by-layer assembly method selecting Fe as metal center and 1,3,5-benzenetricarboxylic acid as organic ligand.
30172513	2	53	theme	fine	579:582	arg1	reusability					584:594	fine reusability	579:594	fine reusability (six cycles)	579:607	The as-prepared material exhibited outstanding sensitivity (0.1 fmol/μL), good selectivity (1:20 and 1:50 respectively), excellent ability of size-exclusion (1: 500), fine reusability (six cycles) and great stability (two months) in enriching N-linked glycopeptides and phosphopeptides from tryptic digests of standard proteins by combining HILIC and IMAC.
30172513	2	53	theme	fine	579:582	arg1	cycles					601:606	six cycles	597:606	six cycles	597:606	The as-prepared material exhibited outstanding sensitivity (0.1 fmol/μL), good selectivity (1:20 and 1:50 respectively), excellent ability of size-exclusion (1: 500), fine reusability (six cycles) and great stability (two months) in enriching N-linked glycopeptides and phosphopeptides from tryptic digests of standard proteins by combining HILIC and IMAC.
30172513	3	54	theme	promising	980:988	arg1	potential					990:998	its promising potential	976:998	its promising potential in enrichment of low-abundance endogenous N-linked glycopeptides and phosphopeptides in practical samples	976:1104	Moreover, it was applied into the enrichment of endogenous N-linked glycopeptides and phosphopeptides in human saliva and achieved great results (43 phosphopeptides and 39 N-linked glycopeptides), revealing its promising potential in enrichment of low-abundance endogenous N-linked glycopeptides and phosphopeptides in practical samples.
30172513	3	55	from	enrichment	803:812	arg1	saliva					880:885	human saliva	874:885	human saliva	874:885	Moreover, it was applied into the enrichment of endogenous N-linked glycopeptides and phosphopeptides in human saliva and achieved great results (43 phosphopeptides and 39 N-linked glycopeptides), revealing its promising potential in enrichment of low-abundance endogenous N-linked glycopeptides and phosphopeptides in practical samples.
30172513	3	56	dep	results	906:912	arg1	phosphopeptides					918:932	43 phosphopeptides	915:932	43 phosphopeptides	915:932	Moreover, it was applied into the enrichment of endogenous N-linked glycopeptides and phosphopeptides in human saliva and achieved great results (43 phosphopeptides and 39 N-linked glycopeptides), revealing its promising potential in enrichment of low-abundance endogenous N-linked glycopeptides and phosphopeptides in practical samples.
30172513	3	56	dep	results	906:912	arg1	glycopeptides					950:962	39 N-linked glycopeptides	938:962	39 N-linked glycopeptides	938:962	Moreover, it was applied into the enrichment of endogenous N-linked glycopeptides and phosphopeptides in human saliva and achieved great results (43 phosphopeptides and 39 N-linked glycopeptides), revealing its promising potential in enrichment of low-abundance endogenous N-linked glycopeptides and phosphopeptides in practical samples.
30172513	2	57	theme	excellent	533:541	arg1	ability					543:549	excellent ability	533:549	excellent ability of size-exclusion (1: 500)	533:576	The as-prepared material exhibited outstanding sensitivity (0.1 fmol/μL), good selectivity (1:20 and 1:50 respectively), excellent ability of size-exclusion (1: 500), fine reusability (six cycles) and great stability (two months) in enriching N-linked glycopeptides and phosphopeptides from tryptic digests of standard proteins by combining HILIC and IMAC.
30172513	3	58	gly	glycopeptides	1051:1063	arg2	glycopeptides					1051:1063	low-abundance endogenous N-linked glycopeptides	1017:1063	low-abundance endogenous N-linked glycopeptides	1017:1063	Moreover, it was applied into the enrichment of endogenous N-linked glycopeptides and phosphopeptides in human saliva and achieved great results (43 phosphopeptides and 39 N-linked glycopeptides), revealing its promising potential in enrichment of low-abundance endogenous N-linked glycopeptides and phosphopeptides in practical samples.
30172513	0	59	theme	selective	77:85	arg1	enrichment					87:96	highly selective enrichment	70:96	highly selective enrichment of endogenous N-linked glycopeptides and phosphopeptides	70:153	Core-shell structured magnetic metal-organic framework composites for highly selective enrichment of endogenous N-linked glycopeptides and phosphopeptides.
30172513	2	60	theme	proteins	731:738	arg1	digests					711:717	tryptic digests	703:717	tryptic digests of standard proteins	703:738	The as-prepared material exhibited outstanding sensitivity (0.1 fmol/μL), good selectivity (1:20 and 1:50 respectively), excellent ability of size-exclusion (1: 500), fine reusability (six cycles) and great stability (two months) in enriching N-linked glycopeptides and phosphopeptides from tryptic digests of standard proteins by combining HILIC and IMAC.
30172513	1	61	theme	core-shell	170:179	arg1	composites					225:234	core-shell structured magnetic metal-organic framework composites	170:234	core-shell structured magnetic metal-organic framework composites denoted as Fe3O4 @MIL-100(Fe)	170:264	In this work, core-shell structured magnetic metal-organic framework composites denoted as Fe3O4 @MIL-100(Fe) were synthesized by means of a layer-by-layer assembly method selecting Fe as metal center and 1,3,5-benzenetricarboxylic acid as organic ligand.
30172513	1	61	theme	core-shell	170:179	arg1	center					350:355	metal center	344:355	metal center	344:355	In this work, core-shell structured magnetic metal-organic framework composites denoted as Fe3O4 @MIL-100(Fe) were synthesized by means of a layer-by-layer assembly method selecting Fe as metal center and 1,3,5-benzenetricarboxylic acid as organic ligand.
30172513	1	61	theme	core-shell	170:179	arg1	acid					388:391	1,3,5-benzenetricarboxylic acid	361:391	1,3,5-benzenetricarboxylic acid	361:391	In this work, core-shell structured magnetic metal-organic framework composites denoted as Fe3O4 @MIL-100(Fe) were synthesized by means of a layer-by-layer assembly method selecting Fe as metal center and 1,3,5-benzenetricarboxylic acid as organic ligand.
30172513	1	61	theme	core-shell	170:179	arg1	ligand					404:409	organic ligand	396:409	organic ligand	396:409	In this work, core-shell structured magnetic metal-organic framework composites denoted as Fe3O4 @MIL-100(Fe) were synthesized by means of a layer-by-layer assembly method selecting Fe as metal center and 1,3,5-benzenetricarboxylic acid as organic ligand.
30172513	2	62	gly	glycopeptides	664:676	arg1	digests					711:717	tryptic digests	703:717	tryptic digests of standard proteins	703:738	The as-prepared material exhibited outstanding sensitivity (0.1 fmol/μL), good selectivity (1:20 and 1:50 respectively), excellent ability of size-exclusion (1: 500), fine reusability (six cycles) and great stability (two months) in enriching N-linked glycopeptides and phosphopeptides from tryptic digests of standard proteins by combining HILIC and IMAC.
30172513	2	62	gly	glycopeptides	664:676	arg2	glycopeptides					664:676	N-linked glycopeptides	655:676	N-linked glycopeptides	655:676	The as-prepared material exhibited outstanding sensitivity (0.1 fmol/μL), good selectivity (1:20 and 1:50 respectively), excellent ability of size-exclusion (1: 500), fine reusability (six cycles) and great stability (two months) in enriching N-linked glycopeptides and phosphopeptides from tryptic digests of standard proteins by combining HILIC and IMAC.
29522825	0	0	theme	prediction	74:83	arg1	tool					85:88	A Chinese hamster ovary (CHO) genome-specific glycosylation prediction tool	14:88	Glyco-Mapper: A Chinese hamster ovary (CHO) genome-specific glycosylation prediction tool.	0:89	Glyco-Mapper: A Chinese hamster ovary (CHO) genome-specific glycosylation prediction tool.
29522825	5	1	dep	accuracy	1182:1189	arg1	an					1179:1180	an	1179:1180	an	1179:1180	A glycoprotein-producing CHO cell line reference glycoform was modeled and a novel Glyco-Mapper prediction was experimentally confirmed with an accuracy and specificity of 95% and 98%, respectively.
29522825	4	2	theme	large	839:843	arg1	range					845:849	a large range	837:849	a large range of glycoform engineering strategies, including the altered expression of glycosylation, nucleotide sugar transport, and metabolism genes, as well as an altered nutrient feeding strategy	837:1035	The modeled glycoforms span a large range of glycoform engineering strategies, including the altered expression of glycosylation, nucleotide sugar transport, and metabolism genes, as well as an altered nutrient feeding strategy.
29522825	4	2	theme	large	839:843	arg1	expression					910:919	the altered expression	898:919	the altered expression of glycosylation, nucleotide sugar transport, and metabolism genes	898:986	The modeled glycoforms span a large range of glycoform engineering strategies, including the altered expression of glycosylation, nucleotide sugar transport, and metabolism genes, as well as an altered nutrient feeding strategy.
29522825	6	3	theme	prediction	1271:1280	arg1	Glyco-Mapper					1237:1248	Glyco-Mapper	1237:1248	Glyco-Mapper	1237:1248	Glyco-Mapper is a product quality prediction tool that provides a streamlined way to design host cell line genomes to achieve specific product quality attributes.
29522825	6	3	theme	prediction	1271:1280	arg1	tool					1282:1285	a product quality prediction tool	1253:1285	a product quality prediction tool that provides a streamlined way to design host cell line genomes to achieve specific product quality attributes	1253:1397	Glyco-Mapper is a product quality prediction tool that provides a streamlined way to design host cell line genomes to achieve specific product quality attributes.
29522825	1	4	theme	prediction	147:156	arg1	Glyco-Mapper					91:102	Glyco-Mapper	91:102	Glyco-Mapper	91:102	Glyco-Mapper is a novel systems biology product quality prediction tool created using a new framework termed: Discretized Reaction Network Modeling using Fuzzy Parameters (DReaM-zyP).
29522825	1	4	theme	prediction	147:156	arg1	tool					158:161	a novel systems biology product quality prediction tool	107:161	a novel systems biology product quality prediction tool created using a new framework termed: Discretized Reaction Network Modeling using Fuzzy Parameters (DReaM-zyP)	107:272	Glyco-Mapper is a novel systems biology product quality prediction tool created using a new framework termed: Discretized Reaction Network Modeling using Fuzzy Parameters (DReaM-zyP).
29522825	4	5	theme	nutrient	1011:1018	arg1	strategy					1028:1035	an altered nutrient feeding strategy	1000:1035	a large range of glycoform engineering strategies, including the altered expression of glycosylation, nucleotide sugar transport, and metabolism genes, as well as an altered nutrient feeding strategy	837:1035	The modeled glycoforms span a large range of glycoform engineering strategies, including the altered expression of glycosylation, nucleotide sugar transport, and metabolism genes, as well as an altered nutrient feeding strategy.
29522825	1	6	theme	Fuzzy	245:249	arg1	Parameters					251:260	Fuzzy Parameters	245:260	Fuzzy Parameters (DReaM-zyP)	245:272	Glyco-Mapper is a novel systems biology product quality prediction tool created using a new framework termed: Discretized Reaction Network Modeling using Fuzzy Parameters (DReaM-zyP).
29522825	1	6	theme	Fuzzy	245:249	arg1	DReaM-zyP					263:271	DReaM-zyP	263:271	DReaM-zyP	263:271	Glyco-Mapper is a novel systems biology product quality prediction tool created using a new framework termed: Discretized Reaction Network Modeling using Fuzzy Parameters (DReaM-zyP).
29522825	0	7	theme	glycosylation	60:72	arg1	tool					85:88	A Chinese hamster ovary (CHO) genome-specific glycosylation prediction tool	14:88	Glyco-Mapper: A Chinese hamster ovary (CHO) genome-specific glycosylation prediction tool.	0:89	Glyco-Mapper: A Chinese hamster ovary (CHO) genome-specific glycosylation prediction tool.
29522825	6	8	theme	product	1255:1261	arg1	Glyco-Mapper					1237:1248	Glyco-Mapper	1237:1248	Glyco-Mapper	1237:1248	Glyco-Mapper is a product quality prediction tool that provides a streamlined way to design host cell line genomes to achieve specific product quality attributes.
29522825	6	8	theme	product	1255:1261	arg1	tool					1282:1285	a product quality prediction tool	1253:1285	a product quality prediction tool that provides a streamlined way to design host cell line genomes to achieve specific product quality attributes	1253:1397	Glyco-Mapper is a product quality prediction tool that provides a streamlined way to design host cell line genomes to achieve specific product quality attributes.
29522825	6	9	theme	host	1329:1332	arg1	genomes					1344:1350	host cell line genomes	1329:1350	host cell line genomes	1329:1350	Glyco-Mapper is a product quality prediction tool that provides a streamlined way to design host cell line genomes to achieve specific product quality attributes.
29522825	2	10	theme	feed	319:322	arg1	composition					324:334	the nutrient feed composition	306:334	the nutrient feed composition	306:334	Within Glyco-Mapper, users fix the nutrient feed composition and the glycosylation reaction fluxes to fit the model glycoform to the reference experimental glycoform, enabling cell-line specific glycoform predictions as a result of cell engineering strategies.
29522825	4	11	theme	glycosylation	924:936	arg1	expression					910:919	the altered expression	898:919	the altered expression of glycosylation, nucleotide sugar transport, and metabolism genes	898:986	The modeled glycoforms span a large range of glycoform engineering strategies, including the altered expression of glycosylation, nucleotide sugar transport, and metabolism genes, as well as an altered nutrient feeding strategy.
29522825	5	12	theme	novel	1115:1119	arg1	prediction					1134:1143	a novel Glyco-Mapper prediction	1113:1143	a novel Glyco-Mapper prediction	1113:1143	A glycoprotein-producing CHO cell line reference glycoform was modeled and a novel Glyco-Mapper prediction was experimentally confirmed with an accuracy and specificity of 95% and 98%, respectively.
29522825	2	13	theme	strategies	524:533	arg1	result					497:502	a result	495:502	a result of cell engineering strategies	495:533	Within Glyco-Mapper, users fix the nutrient feed composition and the glycosylation reaction fluxes to fit the model glycoform to the reference experimental glycoform, enabling cell-line specific glycoform predictions as a result of cell engineering strategies.
29522825	2	13	theme	strategies	524:533	arg1	predictions					480:490	cell-line specific glycoform predictions	451:490	cell-line specific glycoform predictions	451:490	Within Glyco-Mapper, users fix the nutrient feed composition and the glycosylation reaction fluxes to fit the model glycoform to the reference experimental glycoform, enabling cell-line specific glycoform predictions as a result of cell engineering strategies.
29522825	3	14	theme	%	738:738	arg1	sensitivity					704:714	sensitivity	704:714	sensitivity	704:714	Glyco-Mapper accurately predicts glycoforms associated with genetic alterations that result in the appearance or disappearance of one or more glycans with an accuracy, sensitivity, and specificity of 96%, 85%, and 97%, respectively, for publications between 1999 and 2014.
29522825	3	14	theme	%	738:738	arg1	specificity					721:731	specificity	721:731	specificity	721:731	Glyco-Mapper accurately predicts glycoforms associated with genetic alterations that result in the appearance or disappearance of one or more glycans with an accuracy, sensitivity, and specificity of 96%, 85%, and 97%, respectively, for publications between 1999 and 2014.
29522825	3	14	theme	%	738:738	arg1	accuracy					694:701	an accuracy	691:701	an accuracy	691:701	Glyco-Mapper accurately predicts glycoforms associated with genetic alterations that result in the appearance or disappearance of one or more glycans with an accuracy, sensitivity, and specificity of 96%, 85%, and 97%, respectively, for publications between 1999 and 2014.
29522825	2	15	theme	glycosylation	344:356	arg1	fluxes					367:372	the glycosylation reaction fluxes	340:372	the glycosylation reaction fluxes	340:372	Within Glyco-Mapper, users fix the nutrient feed composition and the glycosylation reaction fluxes to fit the model glycoform to the reference experimental glycoform, enabling cell-line specific glycoform predictions as a result of cell engineering strategies.
29522825	4	16	theme	metabolism	971:980	arg1	genes					982:986	metabolism genes	971:986	metabolism genes	971:986	The modeled glycoforms span a large range of glycoform engineering strategies, including the altered expression of glycosylation, nucleotide sugar transport, and metabolism genes, as well as an altered nutrient feeding strategy.
29522825	2	17	theme	nutrient	310:317	arg1	composition					324:334	the nutrient feed composition	306:334	the nutrient feed composition	306:334	Within Glyco-Mapper, users fix the nutrient feed composition and the glycosylation reaction fluxes to fit the model glycoform to the reference experimental glycoform, enabling cell-line specific glycoform predictions as a result of cell engineering strategies.
29522825	2	18	theme	engineering	512:522	arg1	strategies					524:533	cell engineering strategies	507:533	cell engineering strategies	507:533	Within Glyco-Mapper, users fix the nutrient feed composition and the glycosylation reaction fluxes to fit the model glycoform to the reference experimental glycoform, enabling cell-line specific glycoform predictions as a result of cell engineering strategies.
29522825	5	19	theme	reference	1077:1085	arg1	glycoform					1087:1095	reference glycoform	1077:1095	A glycoprotein-producing CHO cell line reference glycoform	1038:1095	A glycoprotein-producing CHO cell line reference glycoform was modeled and a novel Glyco-Mapper prediction was experimentally confirmed with an accuracy and specificity of 95% and 98%, respectively.
29522825	4	20	theme	modeled	813:819	arg1	glycoforms					821:830	The modeled glycoforms	809:830	The modeled glycoforms	809:830	The modeled glycoforms span a large range of glycoform engineering strategies, including the altered expression of glycosylation, nucleotide sugar transport, and metabolism genes, as well as an altered nutrient feeding strategy.
29522825	2	21	theme	model	385:389	arg1	glycoform					391:399	the model glycoform	381:399	the model glycoform to the reference experimental glycoform	381:439	Within Glyco-Mapper, users fix the nutrient feed composition and the glycosylation reaction fluxes to fit the model glycoform to the reference experimental glycoform, enabling cell-line specific glycoform predictions as a result of cell engineering strategies.
29522825	2	22	theme	cell	507:510	arg1	strategies					524:533	cell engineering strategies	507:533	cell engineering strategies	507:533	Within Glyco-Mapper, users fix the nutrient feed composition and the glycosylation reaction fluxes to fit the model glycoform to the reference experimental glycoform, enabling cell-line specific glycoform predictions as a result of cell engineering strategies.
29522825	5	23	theme	%	1212:1212	arg1	specificity					1195:1205	specificity	1195:1205	specificity	1195:1205	A glycoprotein-producing CHO cell line reference glycoform was modeled and a novel Glyco-Mapper prediction was experimentally confirmed with an accuracy and specificity of 95% and 98%, respectively.
29522825	5	23	theme	%	1212:1212	arg1	accuracy					1182:1189	accuracy	1182:1189	accuracy	1182:1189	A glycoprotein-producing CHO cell line reference glycoform was modeled and a novel Glyco-Mapper prediction was experimentally confirmed with an accuracy and specificity of 95% and 98%, respectively.
29522825	4	24	theme	nucleotide	939:948	arg1	transport					956:964	nucleotide sugar transport	939:964	nucleotide sugar transport	939:964	The modeled glycoforms span a large range of glycoform engineering strategies, including the altered expression of glycosylation, nucleotide sugar transport, and metabolism genes, as well as an altered nutrient feeding strategy.
29522825	4	25	theme	transport	956:964	arg1	expression					910:919	the altered expression	898:919	the altered expression of glycosylation, nucleotide sugar transport, and metabolism genes	898:986	The modeled glycoforms span a large range of glycoform engineering strategies, including the altered expression of glycosylation, nucleotide sugar transport, and metabolism genes, as well as an altered nutrient feeding strategy.
29522825	3	26	theme	genetic	596:602	arg1	alterations					604:614	genetic alterations	596:614	genetic alterations that result in the appearance or disappearance of one or more glycans with an accuracy, sensitivity, and specificity of 96%, 85%, and 97%, respectively, for publications between 1999 and 2014	596:806	Glyco-Mapper accurately predicts glycoforms associated with genetic alterations that result in the appearance or disappearance of one or more glycans with an accuracy, sensitivity, and specificity of 96%, 85%, and 97%, respectively, for publications between 1999 and 2014.
29522825	6	27	theme	quality	1380:1386	arg1	attributes					1388:1397	specific product quality attributes	1363:1397	specific product quality attributes	1363:1397	Glyco-Mapper is a product quality prediction tool that provides a streamlined way to design host cell line genomes to achieve specific product quality attributes.
29522825	1	28	theme	new	179:181	arg1	framework					183:191	a new framework	177:191	a new framework termed: Discretized Reaction Network Modeling using Fuzzy Parameters (DReaM-zyP)	177:272	Glyco-Mapper is a novel systems biology product quality prediction tool created using a new framework termed: Discretized Reaction Network Modeling using Fuzzy Parameters (DReaM-zyP).
29522825	0	29	theme	Chinese	16:22	arg1	ovary					32:36	Chinese hamster ovary	16:36	Chinese hamster ovary	16:36	Glyco-Mapper: A Chinese hamster ovary (CHO) genome-specific glycosylation prediction tool.
29522825	0	30	dep	genome-specific	44:58	arg1	ovary					32:36	Chinese hamster ovary	16:36	Chinese hamster ovary	16:36	Glyco-Mapper: A Chinese hamster ovary (CHO) genome-specific glycosylation prediction tool.
29522825	2	31	theme	reference	408:416	arg1	glycoform					431:439	the reference experimental glycoform	404:439	the reference experimental glycoform	404:439	Within Glyco-Mapper, users fix the nutrient feed composition and the glycosylation reaction fluxes to fit the model glycoform to the reference experimental glycoform, enabling cell-line specific glycoform predictions as a result of cell engineering strategies.
29522825	2	32	theme	experimental	418:429	arg1	glycoform					431:439	the reference experimental glycoform	404:439	the reference experimental glycoform	404:439	Within Glyco-Mapper, users fix the nutrient feed composition and the glycosylation reaction fluxes to fit the model glycoform to the reference experimental glycoform, enabling cell-line specific glycoform predictions as a result of cell engineering strategies.
29522825	6	33	theme	product	1372:1378	arg1	attributes					1388:1397	specific product quality attributes	1363:1397	specific product quality attributes	1363:1397	Glyco-Mapper is a product quality prediction tool that provides a streamlined way to design host cell line genomes to achieve specific product quality attributes.
29522825	4	34	theme	altered	902:908	arg1	expression					910:919	the altered expression	898:919	the altered expression of glycosylation, nucleotide sugar transport, and metabolism genes	898:986	The modeled glycoforms span a large range of glycoform engineering strategies, including the altered expression of glycosylation, nucleotide sugar transport, and metabolism genes, as well as an altered nutrient feeding strategy.
29522825	5	35	gly	glycoform	1087:1095	arg1	reference					1077:1085	reference glycoform	1077:1095	A glycoprotein-producing CHO cell line reference glycoform	1038:1095	A glycoprotein-producing CHO cell line reference glycoform was modeled and a novel Glyco-Mapper prediction was experimentally confirmed with an accuracy and specificity of 95% and 98%, respectively.
29522825	4	36	theme	genes	982:986	arg1	expression					910:919	the altered expression	898:919	the altered expression of glycosylation, nucleotide sugar transport, and metabolism genes	898:986	The modeled glycoforms span a large range of glycoform engineering strategies, including the altered expression of glycosylation, nucleotide sugar transport, and metabolism genes, as well as an altered nutrient feeding strategy.
29522825	6	37	theme	streamlined	1303:1313	arg1	way					1315:1317	a streamlined way	1301:1317	a streamlined way to design host cell line genomes to achieve specific product quality attributes	1301:1397	Glyco-Mapper is a product quality prediction tool that provides a streamlined way to design host cell line genomes to achieve specific product quality attributes.
29522825	3	38	theme	glycans	678:684	arg1	disappearance					649:661	disappearance	649:661	disappearance of one or more glycans with an accuracy, sensitivity, and specificity of 96%, 85%, and 97%, respectively, for publications between 1999 and 2014	649:806	Glyco-Mapper accurately predicts glycoforms associated with genetic alterations that result in the appearance or disappearance of one or more glycans with an accuracy, sensitivity, and specificity of 96%, 85%, and 97%, respectively, for publications between 1999 and 2014.
29522825	3	38	theme	glycans	678:684	arg1	appearance					635:644	the appearance	631:644	the appearance	631:644	Glyco-Mapper accurately predicts glycoforms associated with genetic alterations that result in the appearance or disappearance of one or more glycans with an accuracy, sensitivity, and specificity of 96%, 85%, and 97%, respectively, for publications between 1999 and 2014.
29522825	6	39	theme	quality	1263:1269	arg1	Glyco-Mapper					1237:1248	Glyco-Mapper	1237:1248	Glyco-Mapper	1237:1248	Glyco-Mapper is a product quality prediction tool that provides a streamlined way to design host cell line genomes to achieve specific product quality attributes.
29522825	6	39	theme	quality	1263:1269	arg1	tool					1282:1285	a product quality prediction tool	1253:1285	a product quality prediction tool that provides a streamlined way to design host cell line genomes to achieve specific product quality attributes	1253:1397	Glyco-Mapper is a product quality prediction tool that provides a streamlined way to design host cell line genomes to achieve specific product quality attributes.
29522825	3	40	with	glycans	678:684	arg1	sensitivity					704:714	sensitivity	704:714	sensitivity	704:714	Glyco-Mapper accurately predicts glycoforms associated with genetic alterations that result in the appearance or disappearance of one or more glycans with an accuracy, sensitivity, and specificity of 96%, 85%, and 97%, respectively, for publications between 1999 and 2014.
29522825	3	40	with	glycans	678:684	arg1	specificity					721:731	specificity	721:731	specificity	721:731	Glyco-Mapper accurately predicts glycoforms associated with genetic alterations that result in the appearance or disappearance of one or more glycans with an accuracy, sensitivity, and specificity of 96%, 85%, and 97%, respectively, for publications between 1999 and 2014.
29522825	3	40	with	glycans	678:684	arg1	accuracy					694:701	an accuracy	691:701	an accuracy	691:701	Glyco-Mapper accurately predicts glycoforms associated with genetic alterations that result in the appearance or disappearance of one or more glycans with an accuracy, sensitivity, and specificity of 96%, 85%, and 97%, respectively, for publications between 1999 and 2014.
29522825	0	41	theme	hamster	24:30	arg1	ovary					32:36	Chinese hamster ovary	16:36	Chinese hamster ovary	16:36	Glyco-Mapper: A Chinese hamster ovary (CHO) genome-specific glycosylation prediction tool.
29522825	5	42	theme	Glyco-Mapper	1121:1132	arg1	prediction					1134:1143	a novel Glyco-Mapper prediction	1113:1143	a novel Glyco-Mapper prediction	1113:1143	A glycoprotein-producing CHO cell line reference glycoform was modeled and a novel Glyco-Mapper prediction was experimentally confirmed with an accuracy and specificity of 95% and 98%, respectively.
29522825	2	43	theme	glycoform	470:478	arg1	result					497:502	a result	495:502	a result of cell engineering strategies	495:533	Within Glyco-Mapper, users fix the nutrient feed composition and the glycosylation reaction fluxes to fit the model glycoform to the reference experimental glycoform, enabling cell-line specific glycoform predictions as a result of cell engineering strategies.
29522825	2	43	theme	glycoform	470:478	arg1	predictions					480:490	cell-line specific glycoform predictions	451:490	cell-line specific glycoform predictions	451:490	Within Glyco-Mapper, users fix the nutrient feed composition and the glycosylation reaction fluxes to fit the model glycoform to the reference experimental glycoform, enabling cell-line specific glycoform predictions as a result of cell engineering strategies.
29522825	5	44	theme	CHO	1063:1065	arg1	line					1072:1075	A glycoprotein-producing CHO cell line	1038:1075	A glycoprotein-producing CHO cell line reference glycoform	1038:1095	A glycoprotein-producing CHO cell line reference glycoform was modeled and a novel Glyco-Mapper prediction was experimentally confirmed with an accuracy and specificity of 95% and 98%, respectively.
29522825	4	45	theme	altered	1003:1009	arg1	strategy					1028:1035	an altered nutrient feeding strategy	1000:1035	a large range of glycoform engineering strategies, including the altered expression of glycosylation, nucleotide sugar transport, and metabolism genes, as well as an altered nutrient feeding strategy	837:1035	The modeled glycoforms span a large range of glycoform engineering strategies, including the altered expression of glycosylation, nucleotide sugar transport, and metabolism genes, as well as an altered nutrient feeding strategy.
29522825	1	46	theme	Discretized	201:211	arg1	Modeling					230:237	Discretized Reaction Network Modeling	201:237	Discretized Reaction Network Modeling using Fuzzy Parameters (DReaM-zyP)	201:272	Glyco-Mapper is a novel systems biology product quality prediction tool created using a new framework termed: Discretized Reaction Network Modeling using Fuzzy Parameters (DReaM-zyP).
29522825	2	47	theme	specific	461:468	arg1	result					497:502	a result	495:502	a result of cell engineering strategies	495:533	Within Glyco-Mapper, users fix the nutrient feed composition and the glycosylation reaction fluxes to fit the model glycoform to the reference experimental glycoform, enabling cell-line specific glycoform predictions as a result of cell engineering strategies.
29522825	2	47	theme	specific	461:468	arg1	predictions					480:490	cell-line specific glycoform predictions	451:490	cell-line specific glycoform predictions	451:490	Within Glyco-Mapper, users fix the nutrient feed composition and the glycosylation reaction fluxes to fit the model glycoform to the reference experimental glycoform, enabling cell-line specific glycoform predictions as a result of cell engineering strategies.
29522825	6	48	theme	line	1339:1342	arg1	genomes					1344:1350	host cell line genomes	1329:1350	host cell line genomes	1329:1350	Glyco-Mapper is a product quality prediction tool that provides a streamlined way to design host cell line genomes to achieve specific product quality attributes.
29522825	4	49	theme	engineering	864:874	arg1	strategies					876:885	glycoform engineering strategies	854:885	glycoform engineering strategies	854:885	The modeled glycoforms span a large range of glycoform engineering strategies, including the altered expression of glycosylation, nucleotide sugar transport, and metabolism genes, as well as an altered nutrient feeding strategy.
29522825	4	49	theme	engineering	864:874	arg1	expression					910:919	the altered expression	898:919	the altered expression of glycosylation, nucleotide sugar transport, and metabolism genes	898:986	The modeled glycoforms span a large range of glycoform engineering strategies, including the altered expression of glycosylation, nucleotide sugar transport, and metabolism genes, as well as an altered nutrient feeding strategy.
29522825	5	50	theme	cell	1067:1070	arg1	line					1072:1075	A glycoprotein-producing CHO cell line	1038:1075	A glycoprotein-producing CHO cell line reference glycoform	1038:1095	A glycoprotein-producing CHO cell line reference glycoform was modeled and a novel Glyco-Mapper prediction was experimentally confirmed with an accuracy and specificity of 95% and 98%, respectively.
29522825	2	51	theme	reaction	358:365	arg1	fluxes					367:372	the glycosylation reaction fluxes	340:372	the glycosylation reaction fluxes	340:372	Within Glyco-Mapper, users fix the nutrient feed composition and the glycosylation reaction fluxes to fit the model glycoform to the reference experimental glycoform, enabling cell-line specific glycoform predictions as a result of cell engineering strategies.
29522825	5	52	dep	line	1072:1075	arg1	glycoform					1087:1095	reference glycoform	1077:1095	A glycoprotein-producing CHO cell line reference glycoform	1038:1095	A glycoprotein-producing CHO cell line reference glycoform was modeled and a novel Glyco-Mapper prediction was experimentally confirmed with an accuracy and specificity of 95% and 98%, respectively.
29522825	4	53	theme	glycoform	854:862	arg1	strategies					876:885	glycoform engineering strategies	854:885	glycoform engineering strategies	854:885	The modeled glycoforms span a large range of glycoform engineering strategies, including the altered expression of glycosylation, nucleotide sugar transport, and metabolism genes, as well as an altered nutrient feeding strategy.
29522825	4	53	theme	glycoform	854:862	arg1	expression					910:919	the altered expression	898:919	the altered expression of glycosylation, nucleotide sugar transport, and metabolism genes	898:986	The modeled glycoforms span a large range of glycoform engineering strategies, including the altered expression of glycosylation, nucleotide sugar transport, and metabolism genes, as well as an altered nutrient feeding strategy.
29522825	2	54	theme	cell-line	451:459	arg1	result					497:502	a result	495:502	a result of cell engineering strategies	495:533	Within Glyco-Mapper, users fix the nutrient feed composition and the glycosylation reaction fluxes to fit the model glycoform to the reference experimental glycoform, enabling cell-line specific glycoform predictions as a result of cell engineering strategies.
29522825	2	54	theme	cell-line	451:459	arg1	predictions					480:490	cell-line specific glycoform predictions	451:490	cell-line specific glycoform predictions	451:490	Within Glyco-Mapper, users fix the nutrient feed composition and the glycosylation reaction fluxes to fit the model glycoform to the reference experimental glycoform, enabling cell-line specific glycoform predictions as a result of cell engineering strategies.
29522825	1	55	theme	novel	109:113	arg1	Glyco-Mapper					91:102	Glyco-Mapper	91:102	Glyco-Mapper	91:102	Glyco-Mapper is a novel systems biology product quality prediction tool created using a new framework termed: Discretized Reaction Network Modeling using Fuzzy Parameters (DReaM-zyP).
29522825	1	55	theme	novel	109:113	arg1	tool					158:161	a novel systems biology product quality prediction tool	107:161	a novel systems biology product quality prediction tool created using a new framework termed: Discretized Reaction Network Modeling using Fuzzy Parameters (DReaM-zyP)	107:272	Glyco-Mapper is a novel systems biology product quality prediction tool created using a new framework termed: Discretized Reaction Network Modeling using Fuzzy Parameters (DReaM-zyP).
29522825	0	56	dep	Glyco-Mapper	0:11	arg1	tool					85:88	A Chinese hamster ovary (CHO) genome-specific glycosylation prediction tool	14:88	Glyco-Mapper: A Chinese hamster ovary (CHO) genome-specific glycosylation prediction tool.	0:89	Glyco-Mapper: A Chinese hamster ovary (CHO) genome-specific glycosylation prediction tool.
29522825	1	57	theme	systems	115:121	arg1	Glyco-Mapper					91:102	Glyco-Mapper	91:102	Glyco-Mapper	91:102	Glyco-Mapper is a novel systems biology product quality prediction tool created using a new framework termed: Discretized Reaction Network Modeling using Fuzzy Parameters (DReaM-zyP).
29522825	1	57	theme	systems	115:121	arg1	tool					158:161	a novel systems biology product quality prediction tool	107:161	a novel systems biology product quality prediction tool created using a new framework termed: Discretized Reaction Network Modeling using Fuzzy Parameters (DReaM-zyP)	107:272	Glyco-Mapper is a novel systems biology product quality prediction tool created using a new framework termed: Discretized Reaction Network Modeling using Fuzzy Parameters (DReaM-zyP).
29522825	1	58	theme	Reaction	213:220	arg1	Modeling					230:237	Discretized Reaction Network Modeling	201:237	Discretized Reaction Network Modeling using Fuzzy Parameters (DReaM-zyP)	201:272	Glyco-Mapper is a novel systems biology product quality prediction tool created using a new framework termed: Discretized Reaction Network Modeling using Fuzzy Parameters (DReaM-zyP).
29522825	4	59	theme	strategies	876:885	arg1	strategy					1028:1035	an altered nutrient feeding strategy	1000:1035	a large range of glycoform engineering strategies, including the altered expression of glycosylation, nucleotide sugar transport, and metabolism genes, as well as an altered nutrient feeding strategy	837:1035	The modeled glycoforms span a large range of glycoform engineering strategies, including the altered expression of glycosylation, nucleotide sugar transport, and metabolism genes, as well as an altered nutrient feeding strategy.
29522825	4	59	theme	strategies	876:885	arg1	range					845:849	a large range	837:849	a large range of glycoform engineering strategies, including the altered expression of glycosylation, nucleotide sugar transport, and metabolism genes, as well as an altered nutrient feeding strategy	837:1035	The modeled glycoforms span a large range of glycoform engineering strategies, including the altered expression of glycosylation, nucleotide sugar transport, and metabolism genes, as well as an altered nutrient feeding strategy.
29522825	4	59	theme	strategies	876:885	arg1	expression					910:919	the altered expression	898:919	the altered expression of glycosylation, nucleotide sugar transport, and metabolism genes	898:986	The modeled glycoforms span a large range of glycoform engineering strategies, including the altered expression of glycosylation, nucleotide sugar transport, and metabolism genes, as well as an altered nutrient feeding strategy.
29522825	6	60	theme	specific	1363:1370	arg1	attributes					1388:1397	specific product quality attributes	1363:1397	specific product quality attributes	1363:1397	Glyco-Mapper is a product quality prediction tool that provides a streamlined way to design host cell line genomes to achieve specific product quality attributes.
29522825	5	61	theme	glycoprotein-producing	1040:1061	arg1	line					1072:1075	A glycoprotein-producing CHO cell line	1038:1075	A glycoprotein-producing CHO cell line reference glycoform	1038:1095	A glycoprotein-producing CHO cell line reference glycoform was modeled and a novel Glyco-Mapper prediction was experimentally confirmed with an accuracy and specificity of 95% and 98%, respectively.
29522825	1	62	theme	biology	123:129	arg1	Glyco-Mapper					91:102	Glyco-Mapper	91:102	Glyco-Mapper	91:102	Glyco-Mapper is a novel systems biology product quality prediction tool created using a new framework termed: Discretized Reaction Network Modeling using Fuzzy Parameters (DReaM-zyP).
29522825	1	62	theme	biology	123:129	arg1	tool					158:161	a novel systems biology product quality prediction tool	107:161	a novel systems biology product quality prediction tool created using a new framework termed: Discretized Reaction Network Modeling using Fuzzy Parameters (DReaM-zyP)	107:272	Glyco-Mapper is a novel systems biology product quality prediction tool created using a new framework termed: Discretized Reaction Network Modeling using Fuzzy Parameters (DReaM-zyP).
29522825	6	63	theme	cell	1334:1337	arg1	genomes					1344:1350	host cell line genomes	1329:1350	host cell line genomes	1329:1350	Glyco-Mapper is a product quality prediction tool that provides a streamlined way to design host cell line genomes to achieve specific product quality attributes.
29522825	1	64	theme	Network	222:228	arg1	Modeling					230:237	Discretized Reaction Network Modeling	201:237	Discretized Reaction Network Modeling using Fuzzy Parameters (DReaM-zyP)	201:272	Glyco-Mapper is a novel systems biology product quality prediction tool created using a new framework termed: Discretized Reaction Network Modeling using Fuzzy Parameters (DReaM-zyP).
29522825	0	65	theme	genome-specific	44:58	arg1	tool					85:88	A Chinese hamster ovary (CHO) genome-specific glycosylation prediction tool	14:88	Glyco-Mapper: A Chinese hamster ovary (CHO) genome-specific glycosylation prediction tool.	0:89	Glyco-Mapper: A Chinese hamster ovary (CHO) genome-specific glycosylation prediction tool.
29522825	1	66	theme	product	131:137	arg1	Glyco-Mapper					91:102	Glyco-Mapper	91:102	Glyco-Mapper	91:102	Glyco-Mapper is a novel systems biology product quality prediction tool created using a new framework termed: Discretized Reaction Network Modeling using Fuzzy Parameters (DReaM-zyP).
29522825	1	66	theme	product	131:137	arg1	tool					158:161	a novel systems biology product quality prediction tool	107:161	a novel systems biology product quality prediction tool created using a new framework termed: Discretized Reaction Network Modeling using Fuzzy Parameters (DReaM-zyP)	107:272	Glyco-Mapper is a novel systems biology product quality prediction tool created using a new framework termed: Discretized Reaction Network Modeling using Fuzzy Parameters (DReaM-zyP).
29522825	4	67	theme	sugar	950:954	arg1	transport					956:964	nucleotide sugar transport	939:964	nucleotide sugar transport	939:964	The modeled glycoforms span a large range of glycoform engineering strategies, including the altered expression of glycosylation, nucleotide sugar transport, and metabolism genes, as well as an altered nutrient feeding strategy.
29522825	2	68	gly	glycoform	431:439	arg1	reference					408:416	the reference experimental glycoform	404:439	the reference experimental glycoform	404:439	Within Glyco-Mapper, users fix the nutrient feed composition and the glycosylation reaction fluxes to fit the model glycoform to the reference experimental glycoform, enabling cell-line specific glycoform predictions as a result of cell engineering strategies.
29522825	1	69	theme	quality	139:145	arg1	Glyco-Mapper					91:102	Glyco-Mapper	91:102	Glyco-Mapper	91:102	Glyco-Mapper is a novel systems biology product quality prediction tool created using a new framework termed: Discretized Reaction Network Modeling using Fuzzy Parameters (DReaM-zyP).
29522825	1	69	theme	quality	139:145	arg1	tool					158:161	a novel systems biology product quality prediction tool	107:161	a novel systems biology product quality prediction tool created using a new framework termed: Discretized Reaction Network Modeling using Fuzzy Parameters (DReaM-zyP)	107:272	Glyco-Mapper is a novel systems biology product quality prediction tool created using a new framework termed: Discretized Reaction Network Modeling using Fuzzy Parameters (DReaM-zyP).
29522825	4	70	theme	feeding	1020:1026	arg1	strategy					1028:1035	an altered nutrient feeding strategy	1000:1035	a large range of glycoform engineering strategies, including the altered expression of glycosylation, nucleotide sugar transport, and metabolism genes, as well as an altered nutrient feeding strategy	837:1035	The modeled glycoforms span a large range of glycoform engineering strategies, including the altered expression of glycosylation, nucleotide sugar transport, and metabolism genes, as well as an altered nutrient feeding strategy.
29522825	5	71	theme	%	1220:1220	arg1	specificity					1195:1205	specificity	1195:1205	specificity	1195:1205	A glycoprotein-producing CHO cell line reference glycoform was modeled and a novel Glyco-Mapper prediction was experimentally confirmed with an accuracy and specificity of 95% and 98%, respectively.
29522825	5	71	theme	%	1220:1220	arg1	accuracy					1182:1189	accuracy	1182:1189	accuracy	1182:1189	A glycoprotein-producing CHO cell line reference glycoform was modeled and a novel Glyco-Mapper prediction was experimentally confirmed with an accuracy and specificity of 95% and 98%, respectively.
31813081	1	0	theme	due	325:327	arg1	diseases					316:323	various diseases	308:323	various diseases due to its chemical composition and protein enrichment	308:378	Sea cucumber, Holothuria scabra, is an echinoderm marine animal that has long been used as a traditional therapeutic in various diseases due to its chemical composition and protein enrichment.
31813081	6	1	theme	transcriptional	1043:1057	arg1	interleukin-1β					1152:1165	interleukin-1β (IL-1β)	1152:1173	interleukin-1β (IL-1β)	1152:1173	It was found that ethyl acetate fraction of H. scabra extracts (EAHS) inhibited pro-inflammatory cytokines synthesis at both the transcriptional and translational levels, notably nitric oxide (NO), inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and prostaglandin E2 (PGE2).
31813081	6	1	theme	transcriptional	1043:1057	arg1	oxide					1100:1104	nitric oxide	1093:1104	nitric oxide (NO)	1093:1109	It was found that ethyl acetate fraction of H. scabra extracts (EAHS) inhibited pro-inflammatory cytokines synthesis at both the transcriptional and translational levels, notably nitric oxide (NO), inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and prostaglandin E2 (PGE2).
31813081	6	1	theme	transcriptional	1043:1057	arg1	necrosis					1182:1189	tumor necrosis factor-α (TNF-α)	1176:1206	tumor necrosis factor-α (TNF-α)	1176:1206	It was found that ethyl acetate fraction of H. scabra extracts (EAHS) inhibited pro-inflammatory cytokines synthesis at both the transcriptional and translational levels, notably nitric oxide (NO), inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and prostaglandin E2 (PGE2).
31813081	6	1	theme	transcriptional	1043:1057	arg1	synthase					1135:1142	inducible nitric oxide synthase	1112:1142	inducible nitric oxide synthase (iNOS)	1112:1149	It was found that ethyl acetate fraction of H. scabra extracts (EAHS) inhibited pro-inflammatory cytokines synthesis at both the transcriptional and translational levels, notably nitric oxide (NO), inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and prostaglandin E2 (PGE2).
31813081	6	1	theme	transcriptional	1043:1057	arg1	E2					1227:1228	prostaglandin E2	1213:1228	prostaglandin E2 (PGE2)	1213:1235	It was found that ethyl acetate fraction of H. scabra extracts (EAHS) inhibited pro-inflammatory cytokines synthesis at both the transcriptional and translational levels, notably nitric oxide (NO), inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and prostaglandin E2 (PGE2).
31813081	6	1	theme	transcriptional	1043:1057	arg1	levels					1077:1082	both the transcriptional and translational levels	1034:1082	levels	1077:1082	It was found that ethyl acetate fraction of H. scabra extracts (EAHS) inhibited pro-inflammatory cytokines synthesis at both the transcriptional and translational levels, notably nitric oxide (NO), inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and prostaglandin E2 (PGE2).
31813081	5	2	theme	RAW264.7	893:900	arg1	macrophage					902:911	RAW264.7 macrophage	893:911	RAW264.7 macrophage	893:911	In this study, we investigated the anti-inflammatory effect of H. scabra extracts by using lipopolysaccharide (LPS) from E. coli to induce an inflammatory response in RAW264.7 macrophage.
31813081	8	3	theme	phenolic	1365:1372	arg1	compound					1374:1381	phenolic compound	1365:1381	phenolic compound	1365:1381	These effects may be influenced by high contents of phenolic compound and triterpene glycosides in EAHS.
31813081	5	4	theme	H.	789:790	arg1	extracts					799:806	H. scabra extracts	789:806	H. scabra extracts	789:806	In this study, we investigated the anti-inflammatory effect of H. scabra extracts by using lipopolysaccharide (LPS) from E. coli to induce an inflammatory response in RAW264.7 macrophage.
31813081	5	5	theme	extracts	799:806	arg1	effect					779:784	the anti-inflammatory effect	757:784	the anti-inflammatory effect of H. scabra extracts	757:806	In this study, we investigated the anti-inflammatory effect of H. scabra extracts by using lipopolysaccharide (LPS) from E. coli to induce an inflammatory response in RAW264.7 macrophage.
31813081	4	6	from	role	604:607	arg1	anti-inflammation					638:654	anti-inflammation	638:654	anti-inflammation	638:654	However, the role of the H. scabra extracts in anti-inflammation and its molecular regulations has not been apparently elucidated yet.
31813081	4	6	from	role	604:607	arg1	regulations					674:684	its molecular regulations	660:684	its molecular regulations	660:684	However, the role of the H. scabra extracts in anti-inflammation and its molecular regulations has not been apparently elucidated yet.
31813081	6	7	theme	cytokines	1011:1019	arg1	synthesis					1021:1029	pro-inflammatory cytokines synthesis	994:1029	pro-inflammatory cytokines synthesis	994:1029	It was found that ethyl acetate fraction of H. scabra extracts (EAHS) inhibited pro-inflammatory cytokines synthesis at both the transcriptional and translational levels, notably nitric oxide (NO), inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and prostaglandin E2 (PGE2).
31813081	8	8	theme	high	1348:1351	arg1	contents					1353:1360	high contents	1348:1360	high contents of phenolic compound	1348:1381	These effects may be influenced by high contents of phenolic compound and triterpene glycosides in EAHS.
31813081	6	9	theme	oxide	1129:1133	arg1	levels					1077:1082	both the transcriptional and translational levels	1034:1082	levels	1077:1082	It was found that ethyl acetate fraction of H. scabra extracts (EAHS) inhibited pro-inflammatory cytokines synthesis at both the transcriptional and translational levels, notably nitric oxide (NO), inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and prostaglandin E2 (PGE2).
31813081	6	9	theme	oxide	1129:1133	arg1	iNOS					1145:1148	iNOS	1145:1148	iNOS	1145:1148	It was found that ethyl acetate fraction of H. scabra extracts (EAHS) inhibited pro-inflammatory cytokines synthesis at both the transcriptional and translational levels, notably nitric oxide (NO), inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and prostaglandin E2 (PGE2).
31813081	6	9	theme	oxide	1129:1133	arg1	synthase					1135:1142	inducible nitric oxide synthase	1112:1142	inducible nitric oxide synthase (iNOS)	1112:1149	It was found that ethyl acetate fraction of H. scabra extracts (EAHS) inhibited pro-inflammatory cytokines synthesis at both the transcriptional and translational levels, notably nitric oxide (NO), inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and prostaglandin E2 (PGE2).
31813081	9	10	theme	natural	1480:1486	arg1	agent					1506:1510	a natural anti-inflammatory agent	1478:1510	a natural anti-inflammatory agent	1478:1510	Therefore, EAHS might have the potential to be developed as a natural anti-inflammatory agent.
31813081	1	11	theme	chemical	336:343	arg1	composition					345:355	its chemical composition	332:355	its chemical composition	332:355	Sea cucumber, Holothuria scabra, is an echinoderm marine animal that has long been used as a traditional therapeutic in various diseases due to its chemical composition and protein enrichment.
31813081	1	12	from	therapeutic	293:303	arg1	diseases					316:323	various diseases	308:323	various diseases due to its chemical composition and protein enrichment	308:378	Sea cucumber, Holothuria scabra, is an echinoderm marine animal that has long been used as a traditional therapeutic in various diseases due to its chemical composition and protein enrichment.
31813081	5	13	from	response	881:888	arg1	macrophage					902:911	RAW264.7 macrophage	893:911	RAW264.7 macrophage	893:911	In this study, we investigated the anti-inflammatory effect of H. scabra extracts by using lipopolysaccharide (LPS) from E. coli to induce an inflammatory response in RAW264.7 macrophage.
31813081	6	14	theme	pro-inflammatory	994:1009	arg1	cytokines					1011:1019	pro-inflammatory cytokines	994:1019	pro-inflammatory cytokines synthesis	994:1029	It was found that ethyl acetate fraction of H. scabra extracts (EAHS) inhibited pro-inflammatory cytokines synthesis at both the transcriptional and translational levels, notably nitric oxide (NO), inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and prostaglandin E2 (PGE2).
31813081	0	15	theme	nitric	116:121	arg1	oxide					123:127	nitric oxide	116:127	nitric oxide	116:127	An ethyl-acetate fraction of Holothuria scabra modulates inflammation in vitro through inhibiting the production of nitric oxide and pro-inflammatory cytokines via NF-κB and JNK pathways.
31813081	6	16	theme	prostaglandin	1213:1225	arg1	levels					1077:1082	both the transcriptional and translational levels	1034:1082	levels	1077:1082	It was found that ethyl acetate fraction of H. scabra extracts (EAHS) inhibited pro-inflammatory cytokines synthesis at both the transcriptional and translational levels, notably nitric oxide (NO), inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and prostaglandin E2 (PGE2).
31813081	6	16	theme	prostaglandin	1213:1225	arg1	PGE2					1231:1234	PGE2	1231:1234	PGE2	1231:1234	It was found that ethyl acetate fraction of H. scabra extracts (EAHS) inhibited pro-inflammatory cytokines synthesis at both the transcriptional and translational levels, notably nitric oxide (NO), inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and prostaglandin E2 (PGE2).
31813081	6	16	theme	prostaglandin	1213:1225	arg1	E2					1227:1228	prostaglandin E2	1213:1228	prostaglandin E2 (PGE2)	1213:1235	It was found that ethyl acetate fraction of H. scabra extracts (EAHS) inhibited pro-inflammatory cytokines synthesis at both the transcriptional and translational levels, notably nitric oxide (NO), inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and prostaglandin E2 (PGE2).
31813081	4	17	theme	extracts	626:633	arg1	role					604:607	the role	600:607	the role of the H. scabra extracts in anti-inflammation and its molecular regulations	600:684	However, the role of the H. scabra extracts in anti-inflammation and its molecular regulations has not been apparently elucidated yet.
31813081	3	18	theme	complex	521:527	arg1	Inflammation					503:514	Inflammation	503:514	Inflammation	503:514	Inflammation is a complex process involved in pro-/anti-inflammatory cytokine products.
31813081	3	18	theme	complex	521:527	arg1	process					529:535	a complex process	519:535	a complex process involved in pro-/anti-inflammatory cytokine products	519:588	Inflammation is a complex process involved in pro-/anti-inflammatory cytokine products.
31813081	4	19	theme	molecular	664:672	arg1	regulations					674:684	its molecular regulations	660:684	its molecular regulations	660:684	However, the role of the H. scabra extracts in anti-inflammation and its molecular regulations has not been apparently elucidated yet.
31813081	8	20	from	glycosides	1398:1407	arg1	EAHS					1412:1415	EAHS	1412:1415	EAHS	1412:1415	These effects may be influenced by high contents of phenolic compound and triterpene glycosides in EAHS.
31813081	1	21	used	used	271:274	arg2	therapeutic					293:303	therapeutic	293:303	therapeutic	293:303	Sea cucumber, Holothuria scabra, is an echinoderm marine animal that has long been used as a traditional therapeutic in various diseases due to its chemical composition and protein enrichment.
31813081	1	21	used	used	271:274	arg2	cucumber					192:199	Sea cucumber	188:199	Sea cucumber	188:199	Sea cucumber, Holothuria scabra, is an echinoderm marine animal that has long been used as a traditional therapeutic in various diseases due to its chemical composition and protein enrichment.
31813081	1	21	used	used	271:274	arg2	animal					245:250	an echinoderm marine animal	224:250	an echinoderm marine animal that has long been used as a traditional therapeutic in various diseases due to its chemical composition and protein enrichment	224:378	Sea cucumber, Holothuria scabra, is an echinoderm marine animal that has long been used as a traditional therapeutic in various diseases due to its chemical composition and protein enrichment.
31813081	2	22	theme	health	470:475	arg1	benefits					477:484	many health benefits	465:484	many health benefits in recent years	465:500	Many researchers have extensively studied the efficacy of sea cucumber extracts for many health benefits in recent years.
31813081	6	23	theme	nitric	1093:1098	arg1	levels					1077:1082	both the transcriptional and translational levels	1034:1082	levels	1077:1082	It was found that ethyl acetate fraction of H. scabra extracts (EAHS) inhibited pro-inflammatory cytokines synthesis at both the transcriptional and translational levels, notably nitric oxide (NO), inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and prostaglandin E2 (PGE2).
31813081	6	23	theme	nitric	1093:1098	arg1	NO					1107:1108	NO	1107:1108	NO	1107:1108	It was found that ethyl acetate fraction of H. scabra extracts (EAHS) inhibited pro-inflammatory cytokines synthesis at both the transcriptional and translational levels, notably nitric oxide (NO), inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and prostaglandin E2 (PGE2).
31813081	6	23	theme	nitric	1093:1098	arg1	oxide					1100:1104	nitric oxide	1093:1104	nitric oxide (NO)	1093:1109	It was found that ethyl acetate fraction of H. scabra extracts (EAHS) inhibited pro-inflammatory cytokines synthesis at both the transcriptional and translational levels, notably nitric oxide (NO), inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and prostaglandin E2 (PGE2).
31813081	2	24	theme	Many	381:384	arg1	researchers					386:396	Many researchers	381:396	Many researchers	381:396	Many researchers have extensively studied the efficacy of sea cucumber extracts for many health benefits in recent years.
31813081	0	25	theme	ethyl-acetate	3:15	arg1	fraction					17:24	An ethyl-acetate fraction	0:24	An ethyl-acetate fraction of Holothuria scabra	0:45	An ethyl-acetate fraction of Holothuria scabra modulates inflammation in vitro through inhibiting the production of nitric oxide and pro-inflammatory cytokines via NF-κB and JNK pathways.
31813081	1	26	theme	protein	361:367	arg1	enrichment					369:378	protein enrichment	361:378	protein enrichment	361:378	Sea cucumber, Holothuria scabra, is an echinoderm marine animal that has long been used as a traditional therapeutic in various diseases due to its chemical composition and protein enrichment.
31813081	6	27	dep	levels	1077:1082	arg1	both					1034:1037	both	1034:1037	both	1034:1037	It was found that ethyl acetate fraction of H. scabra extracts (EAHS) inhibited pro-inflammatory cytokines synthesis at both the transcriptional and translational levels, notably nitric oxide (NO), inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and prostaglandin E2 (PGE2).
31813081	1	28	theme	Sea	188:190	arg1	cucumber					192:199	Sea cucumber	188:199	Sea cucumber	188:199	Sea cucumber, Holothuria scabra, is an echinoderm marine animal that has long been used as a traditional therapeutic in various diseases due to its chemical composition and protein enrichment.
31813081	1	28	theme	Sea	188:190	arg1	scabra					213:218	Holothuria scabra	202:218	Holothuria scabra	202:218	Sea cucumber, Holothuria scabra, is an echinoderm marine animal that has long been used as a traditional therapeutic in various diseases due to its chemical composition and protein enrichment.
31813081	1	28	theme	Sea	188:190	arg1	animal					245:250	an echinoderm marine animal	224:250	an echinoderm marine animal that has long been used as a traditional therapeutic in various diseases due to its chemical composition and protein enrichment	224:378	Sea cucumber, Holothuria scabra, is an echinoderm marine animal that has long been used as a traditional therapeutic in various diseases due to its chemical composition and protein enrichment.
31813081	2	29	theme	sea	439:441	arg1	extracts					452:459	sea cucumber extracts	439:459	sea cucumber extracts	439:459	Many researchers have extensively studied the efficacy of sea cucumber extracts for many health benefits in recent years.
31813081	6	30	theme	extracts	968:975	arg1	fraction					946:953	ethyl acetate fraction	932:953	ethyl acetate fraction of H. scabra extracts (EAHS)	932:982	It was found that ethyl acetate fraction of H. scabra extracts (EAHS) inhibited pro-inflammatory cytokines synthesis at both the transcriptional and translational levels, notably nitric oxide (NO), inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and prostaglandin E2 (PGE2).
31813081	9	31	theme	anti-inflammatory	1488:1504	arg1	agent					1506:1510	a natural anti-inflammatory agent	1478:1510	a natural anti-inflammatory agent	1478:1510	Therefore, EAHS might have the potential to be developed as a natural anti-inflammatory agent.
31813081	5	32	theme	inflammatory	868:879	arg1	response					881:888	an inflammatory response	865:888	an inflammatory response in RAW264.7 macrophage	865:911	In this study, we investigated the anti-inflammatory effect of H. scabra extracts by using lipopolysaccharide (LPS) from E. coli to induce an inflammatory response in RAW264.7 macrophage.
31813081	6	33	theme	scabra	961:966	arg1	EAHS					978:981	EAHS	978:981	EAHS	978:981	It was found that ethyl acetate fraction of H. scabra extracts (EAHS) inhibited pro-inflammatory cytokines synthesis at both the transcriptional and translational levels, notably nitric oxide (NO), inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and prostaglandin E2 (PGE2).
31813081	6	33	theme	scabra	961:966	arg1	extracts					968:975	H. scabra extracts	958:975	H. scabra extracts (EAHS)	958:982	It was found that ethyl acetate fraction of H. scabra extracts (EAHS) inhibited pro-inflammatory cytokines synthesis at both the transcriptional and translational levels, notably nitric oxide (NO), inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and prostaglandin E2 (PGE2).
31813081	3	34	theme	pro-/anti-inflammatory	549:570	arg1	products					581:588	pro-/anti-inflammatory cytokine products	549:588	pro-/anti-inflammatory cytokine products	549:588	Inflammation is a complex process involved in pro-/anti-inflammatory cytokine products.
31813081	8	35	from	contents	1353:1360	arg1	EAHS					1412:1415	EAHS	1412:1415	EAHS	1412:1415	These effects may be influenced by high contents of phenolic compound and triterpene glycosides in EAHS.
31813081	6	36	theme	H.	958:959	arg1	EAHS					978:981	EAHS	978:981	EAHS	978:981	It was found that ethyl acetate fraction of H. scabra extracts (EAHS) inhibited pro-inflammatory cytokines synthesis at both the transcriptional and translational levels, notably nitric oxide (NO), inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and prostaglandin E2 (PGE2).
31813081	6	36	theme	H.	958:959	arg1	extracts					968:975	H. scabra extracts	958:975	H. scabra extracts (EAHS)	958:982	It was found that ethyl acetate fraction of H. scabra extracts (EAHS) inhibited pro-inflammatory cytokines synthesis at both the transcriptional and translational levels, notably nitric oxide (NO), inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and prostaglandin E2 (PGE2).
31813081	0	37	theme	oxide	123:127	arg1	production					102:111	the production	98:111	the production of nitric oxide and pro-inflammatory cytokines	98:158	An ethyl-acetate fraction of Holothuria scabra modulates inflammation in vitro through inhibiting the production of nitric oxide and pro-inflammatory cytokines via NF-κB and JNK pathways.
31813081	8	38	theme	compound	1374:1381	arg1	contents					1353:1360	high contents	1348:1360	high contents of phenolic compound	1348:1381	These effects may be influenced by high contents of phenolic compound and triterpene glycosides in EAHS.
31813081	8	38	theme	compound	1374:1381	arg1	glycosides					1398:1407	triterpene glycosides	1387:1407	triterpene glycosides in EAHS	1387:1415	These effects may be influenced by high contents of phenolic compound and triterpene glycosides in EAHS.
31813081	3	39	theme	cytokine	572:579	arg1	products					581:588	pro-/anti-inflammatory cytokine products	549:588	pro-/anti-inflammatory cytokine products	549:588	Inflammation is a complex process involved in pro-/anti-inflammatory cytokine products.
31813081	1	40	theme	Holothuria	202:211	arg1	cucumber					192:199	Sea cucumber	188:199	Sea cucumber	188:199	Sea cucumber, Holothuria scabra, is an echinoderm marine animal that has long been used as a traditional therapeutic in various diseases due to its chemical composition and protein enrichment.
31813081	1	40	theme	Holothuria	202:211	arg1	scabra					213:218	Holothuria scabra	202:218	Holothuria scabra	202:218	Sea cucumber, Holothuria scabra, is an echinoderm marine animal that has long been used as a traditional therapeutic in various diseases due to its chemical composition and protein enrichment.
31813081	0	41	theme	scabra	40:45	arg1	fraction					17:24	An ethyl-acetate fraction	0:24	An ethyl-acetate fraction of Holothuria scabra	0:45	An ethyl-acetate fraction of Holothuria scabra modulates inflammation in vitro through inhibiting the production of nitric oxide and pro-inflammatory cytokines via NF-κB and JNK pathways.
31813081	0	42	theme	cytokines	150:158	arg1	production					102:111	the production	98:111	the production of nitric oxide and pro-inflammatory cytokines	98:158	An ethyl-acetate fraction of Holothuria scabra modulates inflammation in vitro through inhibiting the production of nitric oxide and pro-inflammatory cytokines via NF-κB and JNK pathways.
31813081	2	43	from	benefits	477:484	arg1	years					496:500	recent years	489:500	recent years	489:500	Many researchers have extensively studied the efficacy of sea cucumber extracts for many health benefits in recent years.
31813081	6	44	theme	translational	1063:1075	arg1	interleukin-1β					1152:1165	interleukin-1β (IL-1β)	1152:1173	interleukin-1β (IL-1β)	1152:1173	It was found that ethyl acetate fraction of H. scabra extracts (EAHS) inhibited pro-inflammatory cytokines synthesis at both the transcriptional and translational levels, notably nitric oxide (NO), inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and prostaglandin E2 (PGE2).
31813081	6	44	theme	translational	1063:1075	arg1	oxide					1100:1104	nitric oxide	1093:1104	nitric oxide (NO)	1093:1109	It was found that ethyl acetate fraction of H. scabra extracts (EAHS) inhibited pro-inflammatory cytokines synthesis at both the transcriptional and translational levels, notably nitric oxide (NO), inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and prostaglandin E2 (PGE2).
31813081	6	44	theme	translational	1063:1075	arg1	necrosis					1182:1189	tumor necrosis factor-α (TNF-α)	1176:1206	tumor necrosis factor-α (TNF-α)	1176:1206	It was found that ethyl acetate fraction of H. scabra extracts (EAHS) inhibited pro-inflammatory cytokines synthesis at both the transcriptional and translational levels, notably nitric oxide (NO), inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and prostaglandin E2 (PGE2).
31813081	6	44	theme	translational	1063:1075	arg1	synthase					1135:1142	inducible nitric oxide synthase	1112:1142	inducible nitric oxide synthase (iNOS)	1112:1149	It was found that ethyl acetate fraction of H. scabra extracts (EAHS) inhibited pro-inflammatory cytokines synthesis at both the transcriptional and translational levels, notably nitric oxide (NO), inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and prostaglandin E2 (PGE2).
31813081	6	44	theme	translational	1063:1075	arg1	E2					1227:1228	prostaglandin E2	1213:1228	prostaglandin E2 (PGE2)	1213:1235	It was found that ethyl acetate fraction of H. scabra extracts (EAHS) inhibited pro-inflammatory cytokines synthesis at both the transcriptional and translational levels, notably nitric oxide (NO), inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and prostaglandin E2 (PGE2).
31813081	6	44	theme	translational	1063:1075	arg1	levels					1077:1082	both the transcriptional and translational levels	1034:1082	levels	1077:1082	It was found that ethyl acetate fraction of H. scabra extracts (EAHS) inhibited pro-inflammatory cytokines synthesis at both the transcriptional and translational levels, notably nitric oxide (NO), inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and prostaglandin E2 (PGE2).
31813081	2	45	theme	many	465:468	arg1	benefits					477:484	many health benefits	465:484	many health benefits in recent years	465:500	Many researchers have extensively studied the efficacy of sea cucumber extracts for many health benefits in recent years.
31813081	0	46	theme	pro-inflammatory	133:148	arg1	cytokines					150:158	pro-inflammatory cytokines	133:158	pro-inflammatory cytokines	133:158	An ethyl-acetate fraction of Holothuria scabra modulates inflammation in vitro through inhibiting the production of nitric oxide and pro-inflammatory cytokines via NF-κB and JNK pathways.
31813081	8	47	theme	triterpene	1387:1396	arg1	glycosides					1398:1407	triterpene glycosides	1387:1407	triterpene glycosides in EAHS	1387:1415	These effects may be influenced by high contents of phenolic compound and triterpene glycosides in EAHS.
31813081	4	48	theme	scabra	619:624	arg1	extracts					626:633	the H. scabra extracts	612:633	the H. scabra extracts	612:633	However, the role of the H. scabra extracts in anti-inflammation and its molecular regulations has not been apparently elucidated yet.
31813081	1	49	from	diseases	316:323	arg1	therapeutic					293:303	therapeutic	293:303	therapeutic	293:303	Sea cucumber, Holothuria scabra, is an echinoderm marine animal that has long been used as a traditional therapeutic in various diseases due to its chemical composition and protein enrichment.
31813081	1	49	from	diseases	316:323	arg1	animal					245:250	an echinoderm marine animal	224:250	an echinoderm marine animal that has long been used as a traditional therapeutic in various diseases due to its chemical composition and protein enrichment	224:378	Sea cucumber, Holothuria scabra, is an echinoderm marine animal that has long been used as a traditional therapeutic in various diseases due to its chemical composition and protein enrichment.
31813081	6	50	theme	acetate	938:944	arg1	fraction					946:953	ethyl acetate fraction	932:953	ethyl acetate fraction of H. scabra extracts (EAHS)	932:982	It was found that ethyl acetate fraction of H. scabra extracts (EAHS) inhibited pro-inflammatory cytokines synthesis at both the transcriptional and translational levels, notably nitric oxide (NO), inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and prostaglandin E2 (PGE2).
31813081	0	51	theme	NF-κB	164:168	arg1	pathways					178:185	NF-κB and JNK pathways	164:185	pathways	178:185	An ethyl-acetate fraction of Holothuria scabra modulates inflammation in vitro through inhibiting the production of nitric oxide and pro-inflammatory cytokines via NF-κB and JNK pathways.
31813081	5	52	theme	anti-inflammatory	761:777	arg1	effect					779:784	the anti-inflammatory effect	757:784	the anti-inflammatory effect of H. scabra extracts	757:806	In this study, we investigated the anti-inflammatory effect of H. scabra extracts by using lipopolysaccharide (LPS) from E. coli to induce an inflammatory response in RAW264.7 macrophage.
31813081	5	53	from	coli	850:853	arg1	lipopolysaccharide					817:834	lipopolysaccharide	817:834	lipopolysaccharide (LPS) from E. coli	817:853	In this study, we investigated the anti-inflammatory effect of H. scabra extracts by using lipopolysaccharide (LPS) from E. coli to induce an inflammatory response in RAW264.7 macrophage.
31813081	5	53	from	coli	850:853	arg1	LPS					837:839	LPS	837:839	LPS	837:839	In this study, we investigated the anti-inflammatory effect of H. scabra extracts by using lipopolysaccharide (LPS) from E. coli to induce an inflammatory response in RAW264.7 macrophage.
31813081	6	54	theme	nitric	1122:1127	arg1	levels					1077:1082	both the transcriptional and translational levels	1034:1082	levels	1077:1082	It was found that ethyl acetate fraction of H. scabra extracts (EAHS) inhibited pro-inflammatory cytokines synthesis at both the transcriptional and translational levels, notably nitric oxide (NO), inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and prostaglandin E2 (PGE2).
31813081	6	54	theme	nitric	1122:1127	arg1	iNOS					1145:1148	iNOS	1145:1148	iNOS	1145:1148	It was found that ethyl acetate fraction of H. scabra extracts (EAHS) inhibited pro-inflammatory cytokines synthesis at both the transcriptional and translational levels, notably nitric oxide (NO), inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and prostaglandin E2 (PGE2).
31813081	6	54	theme	nitric	1122:1127	arg1	synthase					1135:1142	inducible nitric oxide synthase	1112:1142	inducible nitric oxide synthase (iNOS)	1112:1149	It was found that ethyl acetate fraction of H. scabra extracts (EAHS) inhibited pro-inflammatory cytokines synthesis at both the transcriptional and translational levels, notably nitric oxide (NO), inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and prostaglandin E2 (PGE2).
31813081	2	55	theme	extracts	452:459	arg1	efficacy					427:434	the efficacy	423:434	the efficacy of sea cucumber extracts for many health benefits in recent years	423:500	Many researchers have extensively studied the efficacy of sea cucumber extracts for many health benefits in recent years.
31813081	6	56	theme	ethyl	932:936	arg1	fraction					946:953	ethyl acetate fraction	932:953	ethyl acetate fraction of H. scabra extracts (EAHS)	932:982	It was found that ethyl acetate fraction of H. scabra extracts (EAHS) inhibited pro-inflammatory cytokines synthesis at both the transcriptional and translational levels, notably nitric oxide (NO), inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and prostaglandin E2 (PGE2).
31813081	7	57	from	addition	1241:1248	arg1	able					1260:1263	able	1260:1263	able	1260:1263	In addition, EAHS was able to downregulate IκB/NF-κB, and JNK expressions.
31813081	6	58	theme	inducible	1112:1120	arg1	levels					1077:1082	both the transcriptional and translational levels	1034:1082	levels	1077:1082	It was found that ethyl acetate fraction of H. scabra extracts (EAHS) inhibited pro-inflammatory cytokines synthesis at both the transcriptional and translational levels, notably nitric oxide (NO), inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and prostaglandin E2 (PGE2).
31813081	6	58	theme	inducible	1112:1120	arg1	iNOS					1145:1148	iNOS	1145:1148	iNOS	1145:1148	It was found that ethyl acetate fraction of H. scabra extracts (EAHS) inhibited pro-inflammatory cytokines synthesis at both the transcriptional and translational levels, notably nitric oxide (NO), inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and prostaglandin E2 (PGE2).
31813081	6	58	theme	inducible	1112:1120	arg1	synthase					1135:1142	inducible nitric oxide synthase	1112:1142	inducible nitric oxide synthase (iNOS)	1112:1149	It was found that ethyl acetate fraction of H. scabra extracts (EAHS) inhibited pro-inflammatory cytokines synthesis at both the transcriptional and translational levels, notably nitric oxide (NO), inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and prostaglandin E2 (PGE2).
31813081	1	59	theme	various	308:314	arg1	diseases					316:323	various diseases	308:323	various diseases due to its chemical composition and protein enrichment	308:378	Sea cucumber, Holothuria scabra, is an echinoderm marine animal that has long been used as a traditional therapeutic in various diseases due to its chemical composition and protein enrichment.
31813081	0	60	theme	JNK	174:176	arg1	pathways					178:185	NF-κB and JNK pathways	164:185	pathways	178:185	An ethyl-acetate fraction of Holothuria scabra modulates inflammation in vitro through inhibiting the production of nitric oxide and pro-inflammatory cytokines via NF-κB and JNK pathways.
31813081	6	61	theme	tumor	1176:1180	arg1	levels					1077:1082	both the transcriptional and translational levels	1034:1082	levels	1077:1082	It was found that ethyl acetate fraction of H. scabra extracts (EAHS) inhibited pro-inflammatory cytokines synthesis at both the transcriptional and translational levels, notably nitric oxide (NO), inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and prostaglandin E2 (PGE2).
31813081	6	61	theme	tumor	1176:1180	arg1	necrosis					1182:1189	tumor necrosis factor-α (TNF-α)	1176:1206	tumor necrosis factor-α (TNF-α)	1176:1206	It was found that ethyl acetate fraction of H. scabra extracts (EAHS) inhibited pro-inflammatory cytokines synthesis at both the transcriptional and translational levels, notably nitric oxide (NO), inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and prostaglandin E2 (PGE2).
31813081	5	62	theme	scabra	792:797	arg1	extracts					799:806	H. scabra extracts	789:806	H. scabra extracts	789:806	In this study, we investigated the anti-inflammatory effect of H. scabra extracts by using lipopolysaccharide (LPS) from E. coli to induce an inflammatory response in RAW264.7 macrophage.
31813081	9	63	contain	have	1440:1443	arg1	EAHS					1429:1432	EAHS	1429:1432	EAHS	1429:1432	Therefore, EAHS might have the potential to be developed as a natural anti-inflammatory agent.
31813081	9	63	contain	have	1440:1443	arg2	potential					1449:1457	the potential to be developed as a natural anti-inflammatory agent	1445:1510	the potential to be developed as a natural anti-inflammatory agent	1445:1510	Therefore, EAHS might have the potential to be developed as a natural anti-inflammatory agent.
31813081	7	64	dep	IκB/NF-κB	1281:1289	arg1	expressions					1300:1310	expressions	1300:1310	expressions	1300:1310	In addition, EAHS was able to downregulate IκB/NF-κB, and JNK expressions.
31813081	1	65	theme	echinoderm	227:236	arg1	therapeutic					293:303	therapeutic	293:303	therapeutic	293:303	Sea cucumber, Holothuria scabra, is an echinoderm marine animal that has long been used as a traditional therapeutic in various diseases due to its chemical composition and protein enrichment.
31813081	1	65	theme	echinoderm	227:236	arg1	cucumber					192:199	Sea cucumber	188:199	Sea cucumber	188:199	Sea cucumber, Holothuria scabra, is an echinoderm marine animal that has long been used as a traditional therapeutic in various diseases due to its chemical composition and protein enrichment.
31813081	1	65	theme	echinoderm	227:236	arg1	animal					245:250	an echinoderm marine animal	224:250	an echinoderm marine animal that has long been used as a traditional therapeutic in various diseases due to its chemical composition and protein enrichment	224:378	Sea cucumber, Holothuria scabra, is an echinoderm marine animal that has long been used as a traditional therapeutic in various diseases due to its chemical composition and protein enrichment.
31813081	2	66	theme	recent	489:494	arg1	years					496:500	recent years	489:500	recent years	489:500	Many researchers have extensively studied the efficacy of sea cucumber extracts for many health benefits in recent years.
31813081	4	67	theme	H.	616:617	arg1	extracts					626:633	the H. scabra extracts	612:633	the H. scabra extracts	612:633	However, the role of the H. scabra extracts in anti-inflammation and its molecular regulations has not been apparently elucidated yet.
31813081	7	68	from	able	1260:1263	arg1	addition					1241:1248	addition	1241:1248	addition	1241:1248	In addition, EAHS was able to downregulate IκB/NF-κB, and JNK expressions.
31813081	2	69	theme	cucumber	443:450	arg1	extracts					452:459	sea cucumber extracts	439:459	sea cucumber extracts	439:459	Many researchers have extensively studied the efficacy of sea cucumber extracts for many health benefits in recent years.
31813081	1	70	theme	marine	238:243	arg1	therapeutic					293:303	therapeutic	293:303	therapeutic	293:303	Sea cucumber, Holothuria scabra, is an echinoderm marine animal that has long been used as a traditional therapeutic in various diseases due to its chemical composition and protein enrichment.
31813081	1	70	theme	marine	238:243	arg1	cucumber					192:199	Sea cucumber	188:199	Sea cucumber	188:199	Sea cucumber, Holothuria scabra, is an echinoderm marine animal that has long been used as a traditional therapeutic in various diseases due to its chemical composition and protein enrichment.
31813081	1	70	theme	marine	238:243	arg1	animal					245:250	an echinoderm marine animal	224:250	an echinoderm marine animal that has long been used as a traditional therapeutic in various diseases due to its chemical composition and protein enrichment	224:378	Sea cucumber, Holothuria scabra, is an echinoderm marine animal that has long been used as a traditional therapeutic in various diseases due to its chemical composition and protein enrichment.
31211781	0	0	theme	acetylated	79:88	arg1	α-Synuclein					90:100	acetylated α-Synuclein	79:100	acetylated α-Synuclein	79:100	Identification of N-linked glycans as specific mediators of neuronal uptake of acetylated α-Synuclein.
31211781	7	1	link	N-linked	1066:1073	arg1	glycans					1075:1081	N-linked glycans	1066:1081	N-linked glycans	1066:1081	Importantly, our results are specific to αSacetyl because αSun does not demonstrate sensitivity for N-linked glycans in any of our assays.
31211781	3	2	from	aggregation	437:447	arg1	neurons					460:466	primary neurons	452:466	primary neurons	452:466	Here, we report that αSacetyl is more effective at inducing intracellular aggregation in primary neurons than unmodified αS (αSun).
31211781	4	3	theme	other	705:709	arg1	carbohydrates					711:723	other carbohydrates	705:723	other carbohydrates	705:723	We identify complex N-linked glycans as binding partners for αSacetyl and demonstrate that cellular internalization of αSacetyl is reduced significantly upon cleavage of extracellular N-linked glycans, but not other carbohydrates.
31211781	8	4	theme	uptake	1233:1238	arg1	modulators					1210:1219	specifically-as key modulators	1190:1219	specifically-as key modulators of neuronal uptake of αSacetyl	1190:1250	Our study identifies extracellular N-linked glycans-and the glycoprotein neurexin 1β specifically-as key modulators of neuronal uptake of αSacetyl, drawing attention to the potential therapeutic value of αSacetyl-glycan interactions.
31211781	0	5	link	N-linked	18:25	arg1	glycans					27:33	N-linked glycans	18:33	N-linked glycans	18:33	Identification of N-linked glycans as specific mediators of neuronal uptake of acetylated α-Synuclein.
31211781	4	6	theme	cellular	586:593	arg1	internalization					595:609	cellular internalization	586:609	cellular internalization of αSacetyl	586:621	We identify complex N-linked glycans as binding partners for αSacetyl and demonstrate that cellular internalization of αSacetyl is reduced significantly upon cleavage of extracellular N-linked glycans, but not other carbohydrates.
31211781	0	7	theme	α-Synuclein	90:100	arg1	uptake					69:74	neuronal uptake	60:74	neuronal uptake of acetylated α-Synuclein	60:100	Identification of N-linked glycans as specific mediators of neuronal uptake of acetylated α-Synuclein.
31211781	5	8	theme	αSacetyl	747:754	arg1	binding					736:742	binding	736:742	binding of αSacetyl to N-linked glycans	736:774	We verify binding of αSacetyl to N-linked glycans in vitro, using both isolated glycans and cell-derived proteoliposomes.
31211781	8	9	theme	interactions	1325:1336	arg1	value					1300:1304	the potential therapeutic value	1274:1304	the potential therapeutic value of αSacetyl-glycan interactions	1274:1336	Our study identifies extracellular N-linked glycans-and the glycoprotein neurexin 1β specifically-as key modulators of neuronal uptake of αSacetyl, drawing attention to the potential therapeutic value of αSacetyl-glycan interactions.
31211781	8	10	theme	potential	1278:1286	arg1	value					1300:1304	the potential therapeutic value	1274:1304	the potential therapeutic value of αSacetyl-glycan interactions	1274:1336	Our study identifies extracellular N-linked glycans-and the glycoprotein neurexin 1β specifically-as key modulators of neuronal uptake of αSacetyl, drawing attention to the potential therapeutic value of αSacetyl-glycan interactions.
31211781	3	11	theme	intracellular	423:435	arg1	aggregation					437:447	intracellular aggregation	423:447	intracellular aggregation in primary neurons than unmodified αS (αSun)	423:492	Here, we report that αSacetyl is more effective at inducing intracellular aggregation in primary neurons than unmodified αS (αSun).
31211781	8	12	theme	specifically-as	1190:1204	arg1	modulators					1210:1219	specifically-as key modulators	1190:1219	specifically-as key modulators of neuronal uptake of αSacetyl	1190:1250	Our study identifies extracellular N-linked glycans-and the glycoprotein neurexin 1β specifically-as key modulators of neuronal uptake of αSacetyl, drawing attention to the potential therapeutic value of αSacetyl-glycan interactions.
31211781	4	13	link	N-linked	515:522	arg1	glycans					524:530	complex N-linked glycans	507:530	complex N-linked glycans	507:530	We identify complex N-linked glycans as binding partners for αSacetyl and demonstrate that cellular internalization of αSacetyl is reduced significantly upon cleavage of extracellular N-linked glycans, but not other carbohydrates.
31211781	4	13	link	N-linked	515:522	arg1	partners					543:550	binding partners	535:550	binding partners for αSacetyl	535:563	We identify complex N-linked glycans as binding partners for αSacetyl and demonstrate that cellular internalization of αSacetyl is reduced significantly upon cleavage of extracellular N-linked glycans, but not other carbohydrates.
31211781	8	14	theme	αSacetyl	1243:1250	arg1	uptake					1233:1238	neuronal uptake	1224:1238	neuronal uptake of αSacetyl	1224:1250	Our study identifies extracellular N-linked glycans-and the glycoprotein neurexin 1β specifically-as key modulators of neuronal uptake of αSacetyl, drawing attention to the potential therapeutic value of αSacetyl-glycan interactions.
31211781	8	15	theme	key	1206:1208	arg1	modulators					1210:1219	specifically-as key modulators	1190:1219	specifically-as key modulators of neuronal uptake of αSacetyl	1190:1250	Our study identifies extracellular N-linked glycans-and the glycoprotein neurexin 1β specifically-as key modulators of neuronal uptake of αSacetyl, drawing attention to the potential therapeutic value of αSacetyl-glycan interactions.
31211781	8	16	dep	glycans-and	1149:1159	arg1	neurexin					1178:1185	the glycoprotein neurexin	1161:1185	extracellular N-linked glycans-and the glycoprotein neurexin 1β specifically-as key modulators of neuronal uptake of αSacetyl	1126:1250	Our study identifies extracellular N-linked glycans-and the glycoprotein neurexin 1β specifically-as key modulators of neuronal uptake of αSacetyl, drawing attention to the potential therapeutic value of αSacetyl-glycan interactions.
31211781	5	17	theme	N-linked	759:766	arg1	glycans					768:774	N-linked glycans	759:774	N-linked glycans	759:774	We verify binding of αSacetyl to N-linked glycans in vitro, using both isolated glycans and cell-derived proteoliposomes.
31211781	2	18	from	αS	229:230	arg1	humans					235:240	humans	235:240	humans	235:240	αS in humans is constitutively N-terminally acetylated (αSacetyl), although the impact of this modification is relatively unexplored.
31211781	4	19	theme	glycans	688:694	arg1	cleavage					653:660	cleavage	653:660	cleavage of extracellular N-linked glycans, but not other carbohydrates	653:723	We identify complex N-linked glycans as binding partners for αSacetyl and demonstrate that cellular internalization of αSacetyl is reduced significantly upon cleavage of extracellular N-linked glycans, but not other carbohydrates.
31211781	8	20	theme	glycoprotein	1165:1176	arg1	neurexin					1178:1185	the glycoprotein neurexin	1161:1185	extracellular N-linked glycans-and the glycoprotein neurexin 1β specifically-as key modulators of neuronal uptake of αSacetyl	1126:1250	Our study identifies extracellular N-linked glycans-and the glycoprotein neurexin 1β specifically-as key modulators of neuronal uptake of αSacetyl, drawing attention to the potential therapeutic value of αSacetyl-glycan interactions.
31211781	4	21	theme	N-linked	679:686	arg1	glycans					688:694	extracellular N-linked glycans	665:694	extracellular N-linked glycans	665:694	We identify complex N-linked glycans as binding partners for αSacetyl and demonstrate that cellular internalization of αSacetyl is reduced significantly upon cleavage of extracellular N-linked glycans, but not other carbohydrates.
31211781	0	22	theme	glycans	27:33	arg1	Identification					0:13	Identification	0:13	Identification of N-linked glycans as specific mediators of neuronal uptake of acetylated α-Synuclein.	0:101	Identification of N-linked glycans as specific mediators of neuronal uptake of acetylated α-Synuclein.
31211781	4	23	theme	binding	535:541	arg1	glycans					524:530	complex N-linked glycans	507:530	complex N-linked glycans	507:530	We identify complex N-linked glycans as binding partners for αSacetyl and demonstrate that cellular internalization of αSacetyl is reduced significantly upon cleavage of extracellular N-linked glycans, but not other carbohydrates.
31211781	4	23	theme	binding	535:541	arg1	partners					543:550	binding partners	535:550	binding partners for αSacetyl	535:563	We identify complex N-linked glycans as binding partners for αSacetyl and demonstrate that cellular internalization of αSacetyl is reduced significantly upon cleavage of extracellular N-linked glycans, but not other carbohydrates.
31211781	1	24	theme	Cell-to-cell	103:114	arg1	transmission					116:127	Cell-to-cell transmission	103:127	Cell-to-cell transmission of toxic forms of α-Synuclein (αS)	103:162	Cell-to-cell transmission of toxic forms of α-Synuclein (αS) is thought to underlie disease progression in Parkinson disease.
31211781	1	25	theme	disease	187:193	arg1	progression					195:205	disease progression	187:205	disease progression in Parkinson disease	187:226	Cell-to-cell transmission of toxic forms of α-Synuclein (αS) is thought to underlie disease progression in Parkinson disease.
31211781	0	26	theme	N-linked	18:25	arg1	glycans					27:33	N-linked glycans	18:33	N-linked glycans	18:33	Identification of N-linked glycans as specific mediators of neuronal uptake of acetylated α-Synuclein.
31211781	5	27	theme	cell-derived	818:829	arg1	proteoliposomes					831:845	cell-derived proteoliposomes	818:845	cell-derived proteoliposomes	818:845	We verify binding of αSacetyl to N-linked glycans in vitro, using both isolated glycans and cell-derived proteoliposomes.
31211781	0	28	theme	specific	38:45	arg1	mediators					47:55	specific mediators	38:55	specific mediators of neuronal uptake of acetylated α-Synuclein	38:100	Identification of N-linked glycans as specific mediators of neuronal uptake of acetylated α-Synuclein.
31211781	2	29	theme	modification	324:335	arg1	impact					309:314	the impact	305:314	the impact of this modification	305:335	αS in humans is constitutively N-terminally acetylated (αSacetyl), although the impact of this modification is relatively unexplored.
31211781	7	30	theme	N-linked	1066:1073	arg1	glycans					1075:1081	N-linked glycans	1066:1081	N-linked glycans	1066:1081	Importantly, our results are specific to αSacetyl because αSun does not demonstrate sensitivity for N-linked glycans in any of our assays.
31211781	8	31	theme	therapeutic	1288:1298	arg1	value					1300:1304	the potential therapeutic value	1274:1304	the potential therapeutic value of αSacetyl-glycan interactions	1274:1336	Our study identifies extracellular N-linked glycans-and the glycoprotein neurexin 1β specifically-as key modulators of neuronal uptake of αSacetyl, drawing attention to the potential therapeutic value of αSacetyl-glycan interactions.
31211781	8	32	theme	αSacetyl-glycan	1309:1323	arg1	interactions					1325:1336	αSacetyl-glycan interactions	1309:1336	αSacetyl-glycan interactions	1309:1336	Our study identifies extracellular N-linked glycans-and the glycoprotein neurexin 1β specifically-as key modulators of neuronal uptake of αSacetyl, drawing attention to the potential therapeutic value of αSacetyl-glycan interactions.
31211781	4	33	theme	extracellular	665:677	arg1	glycans					688:694	extracellular N-linked glycans	665:694	extracellular N-linked glycans	665:694	We identify complex N-linked glycans as binding partners for αSacetyl and demonstrate that cellular internalization of αSacetyl is reduced significantly upon cleavage of extracellular N-linked glycans, but not other carbohydrates.
31211781	4	34	theme	N-linked	515:522	arg1	glycans					524:530	complex N-linked glycans	507:530	complex N-linked glycans	507:530	We identify complex N-linked glycans as binding partners for αSacetyl and demonstrate that cellular internalization of αSacetyl is reduced significantly upon cleavage of extracellular N-linked glycans, but not other carbohydrates.
31211781	4	34	theme	N-linked	515:522	arg1	partners					543:550	binding partners	535:550	binding partners for αSacetyl	535:563	We identify complex N-linked glycans as binding partners for αSacetyl and demonstrate that cellular internalization of αSacetyl is reduced significantly upon cleavage of extracellular N-linked glycans, but not other carbohydrates.
31211781	8	35	gly	glycoprotein	1165:1176	arg1	glycoprotein					1165:1176	the glycoprotein neurexin	1161:1185	extracellular N-linked glycans-and the glycoprotein neurexin 1β specifically-as key modulators of neuronal uptake of αSacetyl	1126:1250	Our study identifies extracellular N-linked glycans-and the glycoprotein neurexin 1β specifically-as key modulators of neuronal uptake of αSacetyl, drawing attention to the potential therapeutic value of αSacetyl-glycan interactions.
31211781	4	36	theme	carbohydrates	711:723	arg1	cleavage					653:660	cleavage	653:660	cleavage of extracellular N-linked glycans, but not other carbohydrates	653:723	We identify complex N-linked glycans as binding partners for αSacetyl and demonstrate that cellular internalization of αSacetyl is reduced significantly upon cleavage of extracellular N-linked glycans, but not other carbohydrates.
31211781	4	37	link	N-linked	679:686	arg1	glycans					688:694	extracellular N-linked glycans	665:694	extracellular N-linked glycans	665:694	We identify complex N-linked glycans as binding partners for αSacetyl and demonstrate that cellular internalization of αSacetyl is reduced significantly upon cleavage of extracellular N-linked glycans, but not other carbohydrates.
31211781	3	38	theme	primary	452:458	arg1	neurons					460:466	primary neurons	452:466	primary neurons	452:466	Here, we report that αSacetyl is more effective at inducing intracellular aggregation in primary neurons than unmodified αS (αSun).
31211781	4	39	theme	complex	507:513	arg1	glycans					524:530	complex N-linked glycans	507:530	complex N-linked glycans	507:530	We identify complex N-linked glycans as binding partners for αSacetyl and demonstrate that cellular internalization of αSacetyl is reduced significantly upon cleavage of extracellular N-linked glycans, but not other carbohydrates.
31211781	4	39	theme	complex	507:513	arg1	partners					543:550	binding partners	535:550	binding partners for αSacetyl	535:563	We identify complex N-linked glycans as binding partners for αSacetyl and demonstrate that cellular internalization of αSacetyl is reduced significantly upon cleavage of extracellular N-linked glycans, but not other carbohydrates.
31211781	8	40	theme	N-linked	1140:1147	arg1	glycans-and					1149:1159	extracellular N-linked glycans-and	1126:1159	extracellular N-linked glycans-and the glycoprotein neurexin 1β specifically-as key modulators of neuronal uptake of αSacetyl	1126:1250	Our study identifies extracellular N-linked glycans-and the glycoprotein neurexin 1β specifically-as key modulators of neuronal uptake of αSacetyl, drawing attention to the potential therapeutic value of αSacetyl-glycan interactions.
31211781	6	41	theme	neuronal	884:891	arg1	glycoprotein					893:904	a neuronal glycoprotein	882:904	a neuronal glycoprotein	882:904	Finally, we identify neurexin 1β, a neuronal glycoprotein, as capable of driving glycan-dependent uptake of αSacetyl.
31211781	6	41	theme	neuronal	884:891	arg1	neurexin					869:876	neurexin 1β	869:879	neurexin 1β	869:879	Finally, we identify neurexin 1β, a neuronal glycoprotein, as capable of driving glycan-dependent uptake of αSacetyl.
31211781	5	42	theme	isolated	797:804	arg1	glycans					806:812	isolated glycans	797:812	isolated glycans	797:812	We verify binding of αSacetyl to N-linked glycans in vitro, using both isolated glycans and cell-derived proteoliposomes.
31211781	1	43	theme	toxic	132:136	arg1	forms					138:142	toxic forms	132:142	toxic forms of α-Synuclein (αS)	132:162	Cell-to-cell transmission of toxic forms of α-Synuclein (αS) is thought to underlie disease progression in Parkinson disease.
31211781	6	44	gly	glycoprotein	893:904	arg1	glycoprotein					893:904	a neuronal glycoprotein	882:904	a neuronal glycoprotein	882:904	Finally, we identify neurexin 1β, a neuronal glycoprotein, as capable of driving glycan-dependent uptake of αSacetyl.
31211781	6	44	gly	glycoprotein	893:904	arg1	neurexin					869:876	neurexin 1β	869:879	neurexin 1β	869:879	Finally, we identify neurexin 1β, a neuronal glycoprotein, as capable of driving glycan-dependent uptake of αSacetyl.
31211781	6	45	theme	glycan-dependent	929:944	arg1	uptake					946:951	glycan-dependent uptake	929:951	glycan-dependent uptake of αSacetyl	929:963	Finally, we identify neurexin 1β, a neuronal glycoprotein, as capable of driving glycan-dependent uptake of αSacetyl.
31211781	6	46	theme	αSacetyl	956:963	arg1	uptake					946:951	glycan-dependent uptake	929:951	glycan-dependent uptake of αSacetyl	929:963	Finally, we identify neurexin 1β, a neuronal glycoprotein, as capable of driving glycan-dependent uptake of αSacetyl.
31211781	5	47	link	cell-derived	818:829	arg1	proteoliposomes					831:845	cell-derived proteoliposomes	818:845	cell-derived proteoliposomes	818:845	We verify binding of αSacetyl to N-linked glycans in vitro, using both isolated glycans and cell-derived proteoliposomes.
31211781	1	48	from	progression	195:205	arg1	disease					220:226	Parkinson disease	210:226	Parkinson disease	210:226	Cell-to-cell transmission of toxic forms of α-Synuclein (αS) is thought to underlie disease progression in Parkinson disease.
31211781	1	49	theme	forms	138:142	arg1	transmission					116:127	Cell-to-cell transmission	103:127	Cell-to-cell transmission of toxic forms of α-Synuclein (αS)	103:162	Cell-to-cell transmission of toxic forms of α-Synuclein (αS) is thought to underlie disease progression in Parkinson disease.
31211781	5	50	link	N-linked	759:766	arg1	glycans					768:774	N-linked glycans	759:774	N-linked glycans	759:774	We verify binding of αSacetyl to N-linked glycans in vitro, using both isolated glycans and cell-derived proteoliposomes.
31211781	8	51	link	N-linked	1140:1147	arg1	glycans-and					1149:1159	extracellular N-linked glycans-and	1126:1159	extracellular N-linked glycans-and the glycoprotein neurexin 1β specifically-as key modulators of neuronal uptake of αSacetyl	1126:1250	Our study identifies extracellular N-linked glycans-and the glycoprotein neurexin 1β specifically-as key modulators of neuronal uptake of αSacetyl, drawing attention to the potential therapeutic value of αSacetyl-glycan interactions.
31211781	8	52	theme	extracellular	1126:1138	arg1	glycans-and					1149:1159	extracellular N-linked glycans-and	1126:1159	extracellular N-linked glycans-and the glycoprotein neurexin 1β specifically-as key modulators of neuronal uptake of αSacetyl	1126:1250	Our study identifies extracellular N-linked glycans-and the glycoprotein neurexin 1β specifically-as key modulators of neuronal uptake of αSacetyl, drawing attention to the potential therapeutic value of αSacetyl-glycan interactions.
31211781	3	53	theme	unmodified	473:482	arg1	αSun					488:491	αSun	488:491	αSun	488:491	Here, we report that αSacetyl is more effective at inducing intracellular aggregation in primary neurons than unmodified αS (αSun).
31211781	3	53	theme	unmodified	473:482	arg1	αS					484:485	unmodified αS	473:485	unmodified αS (αSun)	473:492	Here, we report that αSacetyl is more effective at inducing intracellular aggregation in primary neurons than unmodified αS (αSun).
31211781	0	54	theme	uptake	69:74	arg1	mediators					47:55	specific mediators	38:55	specific mediators of neuronal uptake of acetylated α-Synuclein	38:100	Identification of N-linked glycans as specific mediators of neuronal uptake of acetylated α-Synuclein.
31211781	1	55	theme	Parkinson	210:218	arg1	disease					220:226	Parkinson disease	210:226	Parkinson disease	210:226	Cell-to-cell transmission of toxic forms of α-Synuclein (αS) is thought to underlie disease progression in Parkinson disease.
31211781	1	56	theme	α-Synuclein	147:157	arg1	forms					138:142	toxic forms	132:142	toxic forms of α-Synuclein (αS)	132:162	Cell-to-cell transmission of toxic forms of α-Synuclein (αS) is thought to underlie disease progression in Parkinson disease.
31211781	0	57	theme	neuronal	60:67	arg1	uptake					69:74	neuronal uptake	60:74	neuronal uptake of acetylated α-Synuclein	60:100	Identification of N-linked glycans as specific mediators of neuronal uptake of acetylated α-Synuclein.
31211781	8	58	theme	neuronal	1224:1231	arg1	uptake					1233:1238	neuronal uptake	1224:1238	neuronal uptake of αSacetyl	1224:1250	Our study identifies extracellular N-linked glycans-and the glycoprotein neurexin 1β specifically-as key modulators of neuronal uptake of αSacetyl, drawing attention to the potential therapeutic value of αSacetyl-glycan interactions.
31211781	2	59	dep	acetylated	273:282	arg1	αSacetyl					285:292	αSacetyl	285:292	αSacetyl	285:292	αS in humans is constitutively N-terminally acetylated (αSacetyl), although the impact of this modification is relatively unexplored.
31211781	4	60	theme	αSacetyl	614:621	arg1	internalization					595:609	cellular internalization	586:609	cellular internalization of αSacetyl	586:621	We identify complex N-linked glycans as binding partners for αSacetyl and demonstrate that cellular internalization of αSacetyl is reduced significantly upon cleavage of extracellular N-linked glycans, but not other carbohydrates.
31371784	2	0	from	located	386:392	arg1	province					401:408	the province	397:408	the province of Ourense (Spain)	397:427	The sediment was collected from the Lobios Hot Spring located in the province of Ourense (Spain).
31371784	4	1	theme	nr	641:642	arg1	database					644:651	the NCBI protein nr database	624:651	the NCBI protein nr database	624:651	A taxonomic classification that was obtained by comparison to the NCBI protein nr database revealed a dominance of Bacteria (93%), followed by Archaea (6%).
31371784	9	2	theme	novel	1191:1195	arg1	XynA3					1221:1225	XynA3	1221:1225	XynA3	1221:1225	Function-based screening revealed a novel xylanase-encoding gene (XynA3), which was successfully expressed in E. coli BL21.
31371784	9	2	theme	novel	1191:1195	arg1	gene					1215:1218	a novel xylanase-encoding gene	1189:1218	a novel xylanase-encoding gene (XynA3)	1189:1226	Function-based screening revealed a novel xylanase-encoding gene (XynA3), which was successfully expressed in E. coli BL21.
31371784	12	3	theme	ions	1748:1751	arg1	presence					1693:1700	the presence	1689:1700	the presence of β-mercaptoethanol, dithiothreitol and Fe3+ ions	1689:1751	The protein was extremely thermostable and showed 94% remaining activity after incubation at 60 °C for 24 h and over 70% remaining activity after incubation at 70 °C for 24 h. Xylanolytic activity of the XynA3 enzyme was stimulated in the presence of β-mercaptoethanol, dithiothreitol and Fe3+ ions.
31371784	12	4	theme	β-mercaptoethanol	1705:1721	arg1	ions					1748:1751	β-mercaptoethanol, dithiothreitol and Fe3+ ions	1705:1751	β-mercaptoethanol, dithiothreitol and Fe3+ ions	1705:1751	The protein was extremely thermostable and showed 94% remaining activity after incubation at 60 °C for 24 h and over 70% remaining activity after incubation at 70 °C for 24 h. Xylanolytic activity of the XynA3 enzyme was stimulated in the presence of β-mercaptoethanol, dithiothreitol and Fe3+ ions.
31371784	1	5	theme	novel	315:319	arg1	xylanases					321:329	novel xylanases	315:329	novel xylanases	315:329	Here, we describe the metagenome composition of a microbial community in a hot spring sediment as well as a sequence-based and function-based screening of the metagenome for identification of novel xylanases.
31371784	4	6	theme	protein	633:639	arg1	database					644:651	the NCBI protein nr database	624:651	the NCBI protein nr database	624:651	A taxonomic classification that was obtained by comparison to the NCBI protein nr database revealed a dominance of Bacteria (93%), followed by Archaea (6%).
31371784	1	7	theme	xylanases	321:329	arg1	identification					297:310	identification	297:310	identification of novel xylanases	297:329	Here, we describe the metagenome composition of a microbial community in a hot spring sediment as well as a sequence-based and function-based screening of the metagenome for identification of novel xylanases.
31371784	13	8	theme	lower	1826:1830	arg1	proportion					1832:1841	lower proportion	1826:1841	lower proportion of xylotriose and xylose	1826:1866	HPLC analysis showed that XynA3 hydrolyzes xylan forming xylobiose with lower proportion of xylotriose and xylose.
31371784	5	9	theme	archaeal	818:825	arg1	phylum					827:832	the main archaeal phylum	809:832	the main archaeal phylum	809:832	The most abundant bacterial phylum was Acidobacteria (25%), while Thaumarchaeota (5%) was the main archaeal phylum.
31371784	5	9	theme	archaeal	818:825	arg1	Thaumarchaeota					785:798	Thaumarchaeota	785:798	Thaumarchaeota (5%)	785:803	The most abundant bacterial phylum was Acidobacteria (25%), while Thaumarchaeota (5%) was the main archaeal phylum.
31371784	13	10	theme	HPLC	1754:1757	arg1	analysis					1759:1766	HPLC analysis	1754:1766	HPLC analysis	1754:1766	HPLC analysis showed that XynA3 hydrolyzes xylan forming xylobiose with lower proportion of xylotriose and xylose.
31371784	13	11	theme	xylotriose	1846:1855	arg1	proportion					1832:1841	lower proportion	1826:1841	lower proportion of xylotriose and xylose	1826:1866	HPLC analysis showed that XynA3 hydrolyzes xylan forming xylobiose with lower proportion of xylotriose and xylose.
31371784	4	12	theme	taxonomic	564:572	arg1	classification					574:587	A taxonomic classification	562:587	A taxonomic classification that was obtained by comparison to the NCBI protein nr database	562:651	A taxonomic classification that was obtained by comparison to the NCBI protein nr database revealed a dominance of Bacteria (93%), followed by Archaea (6%).
31371784	7	13	theme	CAZy	959:962	arg1	database					964:971	the CAZy database	955:971	the CAZy database	955:971	Open reading frames (ORFs) predicted on these contigs were searched by BLAST against the CAZy database to retrieve xylanase encoding ORFs.
31371784	12	14	theme	24 h.	1624:1628	arg1	activity					1642:1649	24 h. Xylanolytic activity	1624:1649	24 h. Xylanolytic activity of the XynA3 enzyme	1624:1669	The protein was extremely thermostable and showed 94% remaining activity after incubation at 60 °C for 24 h and over 70% remaining activity after incubation at 70 °C for 24 h. Xylanolytic activity of the XynA3 enzyme was stimulated in the presence of β-mercaptoethanol, dithiothreitol and Fe3+ ions.
31371784	12	15	theme	dithiothreitol	1724:1737	arg1	ions					1748:1751	β-mercaptoethanol, dithiothreitol and Fe3+ ions	1705:1751	β-mercaptoethanol, dithiothreitol and Fe3+ ions	1705:1751	The protein was extremely thermostable and showed 94% remaining activity after incubation at 60 °C for 24 h and over 70% remaining activity after incubation at 70 °C for 24 h. Xylanolytic activity of the XynA3 enzyme was stimulated in the presence of β-mercaptoethanol, dithiothreitol and Fe3+ ions.
31371784	0	16	theme	Hot	102:104	arg1	Spring					106:111	the Lobios Hot Spring	91:111	the Lobios Hot Spring	91:111	Microbial diversity analysis and screening for novel xylanase enzymes from the sediment of the Lobios Hot Spring in Spain.
31371784	9	17	theme	xylanase-encoding	1197:1213	arg1	XynA3					1221:1225	XynA3	1221:1225	XynA3	1221:1225	Function-based screening revealed a novel xylanase-encoding gene (XynA3), which was successfully expressed in E. coli BL21.
31371784	9	17	theme	xylanase-encoding	1197:1213	arg1	gene					1215:1218	a novel xylanase-encoding gene	1189:1218	a novel xylanase-encoding gene (XynA3)	1189:1226	Function-based screening revealed a novel xylanase-encoding gene (XynA3), which was successfully expressed in E. coli BL21.
31371784	5	18	theme	abundant	728:735	arg1	Acidobacteria					758:770	Acidobacteria	758:770	Acidobacteria (25%)	758:776	The most abundant bacterial phylum was Acidobacteria (25%), while Thaumarchaeota (5%) was the main archaeal phylum.
31371784	5	18	theme	abundant	728:735	arg1	phylum					747:752	The most abundant bacterial phylum	719:752	The most abundant bacterial phylum	719:752	The most abundant bacterial phylum was Acidobacteria (25%), while Thaumarchaeota (5%) was the main archaeal phylum.
31371784	2	19	theme	located	386:392	arg1	Spring					379:384	the Lobios Hot Spring	364:384	the Lobios Hot Spring located in the province of Ourense (Spain)	364:427	The sediment was collected from the Lobios Hot Spring located in the province of Ourense (Spain).
31371784	2	20	theme	Lobios	368:373	arg1	Spring					379:384	the Lobios Hot Spring	364:384	the Lobios Hot Spring located in the province of Ourense (Spain)	364:427	The sediment was collected from the Lobios Hot Spring located in the province of Ourense (Spain).
31371784	0	21	theme	Lobios	95:100	arg1	Spring					106:111	the Lobios Hot Spring	91:111	the Lobios Hot Spring	91:111	Microbial diversity analysis and screening for novel xylanase enzymes from the sediment of the Lobios Hot Spring in Spain.
31371784	10	22	theme	glycoside	1323:1331	arg1	family					1343:1348	glycoside hydrolase family 11	1323:1351	glycoside hydrolase family 11	1323:1351	The resulting protein (41 kDa), a member of glycoside hydrolase family 11 was purified and biochemically characterized.
31371784	14	23	theme	Specific	1869:1876	arg1	activity					1878:1885	Specific activity	1869:1885	Specific activity of the enzyme	1869:1899	Specific activity of the enzyme was 9080 U/mg for oat arabinoxylan and 5080 U/mg for beechwood xylan, respectively, without cellulase activity.
31371784	1	24	theme	metagenome	145:154	arg1	composition					156:166	the metagenome composition	141:166	the metagenome composition of a microbial community in a hot spring sediment as well as a sequence-based and function-based screening of the metagenome for identification of novel xylanases	141:329	Here, we describe the metagenome composition of a microbial community in a hot spring sediment as well as a sequence-based and function-based screening of the metagenome for identification of novel xylanases.
31371784	0	25	theme	diversity	10:18	arg1	analysis					20:27	Microbial diversity analysis	0:27	Microbial diversity analysis	0:27	Microbial diversity analysis and screening for novel xylanase enzymes from the sediment of the Lobios Hot Spring in Spain.
31371784	4	26	theme	Bacteria	677:684	arg1	dominance					664:672	a dominance	662:672	a dominance of Bacteria (93%)	662:690	A taxonomic classification that was obtained by comparison to the NCBI protein nr database revealed a dominance of Bacteria (93%), followed by Archaea (6%).
31371784	3	27	dep	paired	535:540	arg1	reads					542:546	reads	542:546	reads	542:546	Environmental DNA was extracted and sequenced using Illumina technology, and a total of 3.6 Gbp of clean paired reads was produced.
31371784	0	28	theme	Microbial	0:8	arg1	analysis					20:27	Microbial diversity analysis	0:27	Microbial diversity analysis	0:27	Microbial diversity analysis and screening for novel xylanase enzymes from the sediment of the Lobios Hot Spring in Spain.
31371784	1	29	theme	sequence-based	231:244	arg1	screening					265:273	a sequence-based and function-based screening	229:273	a hot spring sediment as well as a sequence-based and function-based screening of the metagenome for identification of novel xylanases	196:329	Here, we describe the metagenome composition of a microbial community in a hot spring sediment as well as a sequence-based and function-based screening of the metagenome for identification of novel xylanases.
31371784	8	30	theme	functional	1098:1107	arg1	genes					1109:1113	functional genes	1098:1113	functional genes encoding thermostable xylanase enzymes	1098:1152	A metagenomic fosmid library of approximately 150,000 clones was constructed to identify functional genes encoding thermostable xylanase enzymes.
31371784	10	31	theme	resulting	1283:1291	arg1	41 kDa					1302:1307	41 kDa	1302:1307	41 kDa	1302:1307	The resulting protein (41 kDa), a member of glycoside hydrolase family 11 was purified and biochemically characterized.
31371784	10	31	theme	resulting	1283:1291	arg1	protein					1293:1299	The resulting protein	1279:1299	The resulting protein (41 kDa)	1279:1308	The resulting protein (41 kDa), a member of glycoside hydrolase family 11 was purified and biochemically characterized.
31371784	10	31	theme	resulting	1283:1291	arg1	member					1313:1318	a member	1311:1318	a member of glycoside hydrolase family 11	1311:1351	The resulting protein (41 kDa), a member of glycoside hydrolase family 11 was purified and biochemically characterized.
31371784	3	32	theme	Gbp	522:524	arg1	total					509:513	a total	507:513	a total of 3.6 Gbp of clean paired reads	507:546	Environmental DNA was extracted and sequenced using Illumina technology, and a total of 3.6 Gbp of clean paired reads was produced.
31371784	8	33	theme	thermostable	1124:1135	arg1	enzymes					1146:1152	thermostable xylanase enzymes	1124:1152	thermostable xylanase enzymes	1124:1152	A metagenomic fosmid library of approximately 150,000 clones was constructed to identify functional genes encoding thermostable xylanase enzymes.
31371784	8	34	theme	metagenomic	1011:1021	arg1	library					1030:1036	A metagenomic fosmid library	1009:1036	A metagenomic fosmid library of approximately 150,000 clones	1009:1068	A metagenomic fosmid library of approximately 150,000 clones was constructed to identify functional genes encoding thermostable xylanase enzymes.
31371784	11	35	theme	highest	1403:1409	arg1	activity					1411:1418	The highest activity	1399:1418	The highest activity	1399:1418	The highest activity was measured at 80 °C and pH 6.5.
31371784	8	36	theme	xylanase	1137:1144	arg1	enzymes					1146:1152	thermostable xylanase enzymes	1124:1152	thermostable xylanase enzymes	1124:1152	A metagenomic fosmid library of approximately 150,000 clones was constructed to identify functional genes encoding thermostable xylanase enzymes.
31371784	12	37	theme	Xylanolytic	1630:1640	arg1	activity					1642:1649	24 h. Xylanolytic activity	1624:1649	24 h. Xylanolytic activity of the XynA3 enzyme	1624:1669	The protein was extremely thermostable and showed 94% remaining activity after incubation at 60 °C for 24 h and over 70% remaining activity after incubation at 70 °C for 24 h. Xylanolytic activity of the XynA3 enzyme was stimulated in the presence of β-mercaptoethanol, dithiothreitol and Fe3+ ions.
31371784	1	38	theme	function-based	250:263	arg1	screening					265:273	a sequence-based and function-based screening	229:273	a hot spring sediment as well as a sequence-based and function-based screening of the metagenome for identification of novel xylanases	196:329	Here, we describe the metagenome composition of a microbial community in a hot spring sediment as well as a sequence-based and function-based screening of the metagenome for identification of novel xylanases.
31371784	0	39	theme	Spring	106:111	arg1	sediment					79:86	the sediment	75:86	the sediment of the Lobios Hot Spring in Spain	75:120	Microbial diversity analysis and screening for novel xylanase enzymes from the sediment of the Lobios Hot Spring in Spain.
31371784	0	40	from	sediment	79:86	arg1	Spain					116:120	Spain	116:120	Spain	116:120	Microbial diversity analysis and screening for novel xylanase enzymes from the sediment of the Lobios Hot Spring in Spain.
31371784	0	40	from	sediment	79:86	arg1	enzymes					62:68	novel xylanase enzymes	47:68	novel xylanase enzymes from the sediment of the Lobios Hot Spring in Spain	47:120	Microbial diversity analysis and screening for novel xylanase enzymes from the sediment of the Lobios Hot Spring in Spain.
31371784	5	41	theme	main	813:816	arg1	phylum					827:832	the main archaeal phylum	809:832	the main archaeal phylum	809:832	The most abundant bacterial phylum was Acidobacteria (25%), while Thaumarchaeota (5%) was the main archaeal phylum.
31371784	5	41	theme	main	813:816	arg1	Thaumarchaeota					785:798	Thaumarchaeota	785:798	Thaumarchaeota (5%)	785:803	The most abundant bacterial phylum was Acidobacteria (25%), while Thaumarchaeota (5%) was the main archaeal phylum.
31371784	1	42	theme	microbial	173:181	arg1	community					183:191	a microbial community	171:191	a microbial community	171:191	Here, we describe the metagenome composition of a microbial community in a hot spring sediment as well as a sequence-based and function-based screening of the metagenome for identification of novel xylanases.
31371784	14	43	theme	oat	1919:1921	arg1	arabinoxylan					1923:1934	oat arabinoxylan	1919:1934	oat arabinoxylan	1919:1934	Specific activity of the enzyme was 9080 U/mg for oat arabinoxylan and 5080 U/mg for beechwood xylan, respectively, without cellulase activity.
31371784	5	44	theme	bacterial	737:745	arg1	Acidobacteria					758:770	Acidobacteria	758:770	Acidobacteria (25%)	758:776	The most abundant bacterial phylum was Acidobacteria (25%), while Thaumarchaeota (5%) was the main archaeal phylum.
31371784	5	44	theme	bacterial	737:745	arg1	phylum					747:752	The most abundant bacterial phylum	719:752	The most abundant bacterial phylum	719:752	The most abundant bacterial phylum was Acidobacteria (25%), while Thaumarchaeota (5%) was the main archaeal phylum.
31371784	3	45	theme	paired	535:540	arg1	Gbp					522:524	3.6 Gbp	518:524	3.6 Gbp of clean paired reads	518:546	Environmental DNA was extracted and sequenced using Illumina technology, and a total of 3.6 Gbp of clean paired reads was produced.
31371784	1	46	theme	community	183:191	arg1	composition					156:166	the metagenome composition	141:166	the metagenome composition of a microbial community in a hot spring sediment as well as a sequence-based and function-based screening of the metagenome for identification of novel xylanases	141:329	Here, we describe the metagenome composition of a microbial community in a hot spring sediment as well as a sequence-based and function-based screening of the metagenome for identification of novel xylanases.
31371784	8	47	theme	clones	1063:1068	arg1	library					1030:1036	A metagenomic fosmid library	1009:1036	A metagenomic fosmid library of approximately 150,000 clones	1009:1068	A metagenomic fosmid library of approximately 150,000 clones was constructed to identify functional genes encoding thermostable xylanase enzymes.
31371784	10	48	theme	family	1343:1348	arg1	protein					1293:1299	The resulting protein	1279:1299	The resulting protein (41 kDa)	1279:1308	The resulting protein (41 kDa), a member of glycoside hydrolase family 11 was purified and biochemically characterized.
31371784	10	48	theme	family	1343:1348	arg1	member					1313:1318	a member	1311:1318	a member of glycoside hydrolase family 11	1311:1351	The resulting protein (41 kDa), a member of glycoside hydrolase family 11 was purified and biochemically characterized.
31371784	9	49	dep	E.	1265:1266	arg1	coli					1268:1271	coli	1268:1271	coli	1268:1271	Function-based screening revealed a novel xylanase-encoding gene (XynA3), which was successfully expressed in E. coli BL21.
31371784	7	50	theme	reading	875:881	arg1	ORFs					891:894	ORFs	891:894	ORFs	891:894	Open reading frames (ORFs) predicted on these contigs were searched by BLAST against the CAZy database to retrieve xylanase encoding ORFs.
31371784	7	50	theme	reading	875:881	arg1	frames					883:888	Open reading frames	870:888	Open reading frames (ORFs) predicted on these contigs	870:922	Open reading frames (ORFs) predicted on these contigs were searched by BLAST against the CAZy database to retrieve xylanase encoding ORFs.
31371784	2	51	theme	Hot	375:377	arg1	Spring					379:384	the Lobios Hot Spring	364:384	the Lobios Hot Spring located in the province of Ourense (Spain)	364:427	The sediment was collected from the Lobios Hot Spring located in the province of Ourense (Spain).
31371784	0	52	theme	xylanase	53:60	arg1	enzymes					62:68	novel xylanase enzymes	47:68	novel xylanase enzymes from the sediment of the Lobios Hot Spring in Spain	47:120	Microbial diversity analysis and screening for novel xylanase enzymes from the sediment of the Lobios Hot Spring in Spain.
31371784	12	53	theme	XynA3	1658:1662	arg1	enzyme					1664:1669	the XynA3 enzyme	1654:1669	the XynA3 enzyme	1654:1669	The protein was extremely thermostable and showed 94% remaining activity after incubation at 60 °C for 24 h and over 70% remaining activity after incubation at 70 °C for 24 h. Xylanolytic activity of the XynA3 enzyme was stimulated in the presence of β-mercaptoethanol, dithiothreitol and Fe3+ ions.
31371784	0	54	theme	novel	47:51	arg1	enzymes					62:68	novel xylanase enzymes	47:68	novel xylanase enzymes from the sediment of the Lobios Hot Spring in Spain	47:120	Microbial diversity analysis and screening for novel xylanase enzymes from the sediment of the Lobios Hot Spring in Spain.
31371784	1	55	theme	metagenome	282:291	arg1	screening					265:273	a sequence-based and function-based screening	229:273	a hot spring sediment as well as a sequence-based and function-based screening of the metagenome for identification of novel xylanases	196:329	Here, we describe the metagenome composition of a microbial community in a hot spring sediment as well as a sequence-based and function-based screening of the metagenome for identification of novel xylanases.
31371784	1	55	theme	metagenome	282:291	arg1	sediment					209:216	a hot spring sediment	196:216	a hot spring sediment as well as a sequence-based and function-based screening of the metagenome for identification of novel xylanases	196:329	Here, we describe the metagenome composition of a microbial community in a hot spring sediment as well as a sequence-based and function-based screening of the metagenome for identification of novel xylanases.
31371784	9	56	theme	Function-based	1155:1168	arg1	screening					1170:1178	Function-based screening	1155:1178	Function-based screening	1155:1178	Function-based screening revealed a novel xylanase-encoding gene (XynA3), which was successfully expressed in E. coli BL21.
31371784	12	57	theme	Fe3+	1743:1746	arg1	ions					1748:1751	β-mercaptoethanol, dithiothreitol and Fe3+ ions	1705:1751	β-mercaptoethanol, dithiothreitol and Fe3+ ions	1705:1751	The protein was extremely thermostable and showed 94% remaining activity after incubation at 60 °C for 24 h and over 70% remaining activity after incubation at 70 °C for 24 h. Xylanolytic activity of the XynA3 enzyme was stimulated in the presence of β-mercaptoethanol, dithiothreitol and Fe3+ ions.
31371784	12	58	dep	incubation	1600:1609	arg1	stimulated					1675:1684	stimulated	1675:1684	was stimulated in the presence of β-mercaptoethanol, dithiothreitol and Fe3+ ions	1671:1751	The protein was extremely thermostable and showed 94% remaining activity after incubation at 60 °C for 24 h and over 70% remaining activity after incubation at 70 °C for 24 h. Xylanolytic activity of the XynA3 enzyme was stimulated in the presence of β-mercaptoethanol, dithiothreitol and Fe3+ ions.
31371784	10	59	theme	hydrolase	1333:1341	arg1	family					1343:1348	glycoside hydrolase family 11	1323:1351	glycoside hydrolase family 11	1323:1351	The resulting protein (41 kDa), a member of glycoside hydrolase family 11 was purified and biochemically characterized.
31371784	7	60	theme	Open	870:873	arg1	ORFs					891:894	ORFs	891:894	ORFs	891:894	Open reading frames (ORFs) predicted on these contigs were searched by BLAST against the CAZy database to retrieve xylanase encoding ORFs.
31371784	7	60	theme	Open	870:873	arg1	frames					883:888	Open reading frames	870:888	Open reading frames (ORFs) predicted on these contigs	870:922	Open reading frames (ORFs) predicted on these contigs were searched by BLAST against the CAZy database to retrieve xylanase encoding ORFs.
31371784	3	61	theme	Environmental	430:442	arg1	DNA					444:446	Environmental DNA	430:446	Environmental DNA	430:446	Environmental DNA was extracted and sequenced using Illumina technology, and a total of 3.6 Gbp of clean paired reads was produced.
31371784	9	62	theme	E.	1265:1266	arg1	BL21					1273:1276	E. coli BL21	1265:1276	E. coli BL21	1265:1276	Function-based screening revealed a novel xylanase-encoding gene (XynA3), which was successfully expressed in E. coli BL21.
31371784	14	63	theme	enzyme	1894:1899	arg1	activity					1878:1885	Specific activity	1869:1885	Specific activity of the enzyme	1869:1899	Specific activity of the enzyme was 9080 U/mg for oat arabinoxylan and 5080 U/mg for beechwood xylan, respectively, without cellulase activity.
31371784	12	64	theme	enzyme	1664:1669	arg1	activity					1642:1649	24 h. Xylanolytic activity	1624:1649	24 h. Xylanolytic activity of the XynA3 enzyme	1624:1669	The protein was extremely thermostable and showed 94% remaining activity after incubation at 60 °C for 24 h and over 70% remaining activity after incubation at 70 °C for 24 h. Xylanolytic activity of the XynA3 enzyme was stimulated in the presence of β-mercaptoethanol, dithiothreitol and Fe3+ ions.
31371784	1	65	from	composition	156:166	arg1	screening					265:273	a sequence-based and function-based screening	229:273	a hot spring sediment as well as a sequence-based and function-based screening of the metagenome for identification of novel xylanases	196:329	Here, we describe the metagenome composition of a microbial community in a hot spring sediment as well as a sequence-based and function-based screening of the metagenome for identification of novel xylanases.
31371784	1	65	from	composition	156:166	arg1	sediment					209:216	a hot spring sediment	196:216	a hot spring sediment as well as a sequence-based and function-based screening of the metagenome for identification of novel xylanases	196:329	Here, we describe the metagenome composition of a microbial community in a hot spring sediment as well as a sequence-based and function-based screening of the metagenome for identification of novel xylanases.
31371784	3	66	theme	Illumina	482:489	arg1	technology					491:500	Illumina technology	482:500	Illumina technology	482:500	Environmental DNA was extracted and sequenced using Illumina technology, and a total of 3.6 Gbp of clean paired reads was produced.
31371784	1	67	theme	hot	198:200	arg1	sediment					209:216	a hot spring sediment	196:216	a hot spring sediment as well as a sequence-based and function-based screening of the metagenome for identification of novel xylanases	196:329	Here, we describe the metagenome composition of a microbial community in a hot spring sediment as well as a sequence-based and function-based screening of the metagenome for identification of novel xylanases.
31371784	12	68	from	60 °C	1547:1551	arg1	incubation					1533:1542	incubation	1533:1542	incubation at 60 °C for 24 h	1533:1560	The protein was extremely thermostable and showed 94% remaining activity after incubation at 60 °C for 24 h and over 70% remaining activity after incubation at 70 °C for 24 h. Xylanolytic activity of the XynA3 enzyme was stimulated in the presence of β-mercaptoethanol, dithiothreitol and Fe3+ ions.
31371784	8	69	theme	fosmid	1023:1028	arg1	library					1030:1036	A metagenomic fosmid library	1009:1036	A metagenomic fosmid library of approximately 150,000 clones	1009:1068	A metagenomic fosmid library of approximately 150,000 clones was constructed to identify functional genes encoding thermostable xylanase enzymes.
31371784	4	70	theme	NCBI	628:631	arg1	database					644:651	the NCBI protein nr database	624:651	the NCBI protein nr database	624:651	A taxonomic classification that was obtained by comparison to the NCBI protein nr database revealed a dominance of Bacteria (93%), followed by Archaea (6%).
31371784	1	71	theme	spring	202:207	arg1	sediment					209:216	a hot spring sediment	196:216	a hot spring sediment as well as a sequence-based and function-based screening of the metagenome for identification of novel xylanases	196:329	Here, we describe the metagenome composition of a microbial community in a hot spring sediment as well as a sequence-based and function-based screening of the metagenome for identification of novel xylanases.
31371784	14	72	theme	cellulase	1993:2001	arg1	activity					2003:2010	cellulase activity	1993:2010	cellulase activity	1993:2010	Specific activity of the enzyme was 9080 U/mg for oat arabinoxylan and 5080 U/mg for beechwood xylan, respectively, without cellulase activity.
31371784	2	73	from	province	401:408	arg1	located					386:392	located	386:392	located	386:392	The sediment was collected from the Lobios Hot Spring located in the province of Ourense (Spain).
31371784	13	74	theme	xylose	1861:1866	arg1	proportion					1832:1841	lower proportion	1826:1841	lower proportion of xylotriose and xylose	1826:1866	HPLC analysis showed that XynA3 hydrolyzes xylan forming xylobiose with lower proportion of xylotriose and xylose.
31371784	2	75	theme	Ourense	413:419	arg1	province					401:408	the province	397:408	the province of Ourense (Spain)	397:427	The sediment was collected from the Lobios Hot Spring located in the province of Ourense (Spain).
31371784	14	76	theme	beechwood	1954:1962	arg1	xylan					1964:1968	beechwood xylan	1954:1968	beechwood xylan	1954:1968	Specific activity of the enzyme was 9080 U/mg for oat arabinoxylan and 5080 U/mg for beechwood xylan, respectively, without cellulase activity.
29637446	0	0	theme	aging	64:68	arg1	rats					70:73	aging rats	64:73	aging rats	64:73	Effect of high chronic intake of sucrose on liver metabolism in aging rats.
29637446	6	1	dep	plasma	886:891	arg1	content					909:915	lipid content	903:915	lipid content	903:915	Sucrose induced an increase in plasma and liver lipid content, and a stimulation of liver protein synthesis rates.
29637446	4	2	theme	male	452:455	arg1	rats					457:460	22-month-old male rats	439:460	22-month-old male rats fed during 5 months with a diet containing either 13 or 62% sucrose, supplemented or not with rutin, vitamin E, A, D, selenium, and zinc	439:597	Four groups of 22-month-old male rats fed during 5 months with a diet containing either 13 or 62% sucrose, supplemented or not with rutin, vitamin E, A, D, selenium, and zinc were compared.
29637446	5	3	theme	in	800:801	arg1	rates					826:830	in vivo protein synthesis rates	800:830	in vivo protein synthesis rates	800:830	We measured liver macronutrient composition, glycation/oxidative stress, enzyme activities (lipogenesis, β-oxidation, fructokinase), gene expression (enzymes and transcription factors), in vivo protein synthesis rates and plasma parameters.
29637446	4	4	dep	%	520:520	arg1	62					518:519	62	518:519	62	518:519	Four groups of 22-month-old male rats fed during 5 months with a diet containing either 13 or 62% sucrose, supplemented or not with rutin, vitamin E, A, D, selenium, and zinc were compared.
29637446	4	4	dep	%	520:520	arg1	13					512:513	13	512:513	13	512:513	Four groups of 22-month-old male rats fed during 5 months with a diet containing either 13 or 62% sucrose, supplemented or not with rutin, vitamin E, A, D, selenium, and zinc were compared.
29637446	6	5	theme	lipid	903:907	arg1	content					909:915	lipid content	903:915	lipid content	903:915	Sucrose induced an increase in plasma and liver lipid content, and a stimulation of liver protein synthesis rates.
29637446	5	6	theme	enzyme	687:692	arg1	activities					694:703	enzyme activities	687:703	enzyme activities (lipogenesis, β-oxidation, fructokinase)	687:744	We measured liver macronutrient composition, glycation/oxidative stress, enzyme activities (lipogenesis, β-oxidation, fructokinase), gene expression (enzymes and transcription factors), in vivo protein synthesis rates and plasma parameters.
29637446	9	7	theme	old	1439:1441	arg1	animals					1443:1449	old animals	1439:1449	old animals	1439:1449	In conclusion, despite differences in enzymatic regulations, and blunted responses of gene expression, high-sucrose diet was still able to induce a marked increase in liver lipid content in old animals.
29637446	9	8	theme	gene	1335:1338	arg1	expression					1340:1349	gene expression	1335:1349	gene expression	1335:1349	In conclusion, despite differences in enzymatic regulations, and blunted responses of gene expression, high-sucrose diet was still able to induce a marked increase in liver lipid content in old animals.
29637446	3	9	theme	diet	318:321	arg1	effect					293:298	the effect	289:298	the effect of a high-sucrose diet in liver of old rats	289:342	Our aims were to examine the effect of a high-sucrose diet in liver of old rats and also the potential benefices of a polyphenol/micronutrient supplementation.
29637446	0	10	from	Effect	0:5	arg1	metabolism					50:59	liver metabolism	44:59	liver metabolism in aging rats	44:73	Effect of high chronic intake of sucrose on liver metabolism in aging rats.
29637446	9	11	from	differences	1272:1282	arg1	regulations					1297:1307	enzymatic regulations	1287:1307	enzymatic regulations	1287:1307	In conclusion, despite differences in enzymatic regulations, and blunted responses of gene expression, high-sucrose diet was still able to induce a marked increase in liver lipid content in old animals.
29637446	4	12	contain	containing	494:503	arg2	sucrose					522:528	either 13 or 62% sucrose	505:528	either 13 or 62% sucrose	505:528	Four groups of 22-month-old male rats fed during 5 months with a diet containing either 13 or 62% sucrose, supplemented or not with rutin, vitamin E, A, D, selenium, and zinc were compared.
29637446	4	12	contain	containing	494:503	arg1	diet					489:492	a diet	487:492	a diet containing either 13 or 62% sucrose	487:528	Four groups of 22-month-old male rats fed during 5 months with a diet containing either 13 or 62% sucrose, supplemented or not with rutin, vitamin E, A, D, selenium, and zinc were compared.
29637446	9	13	theme	expression	1340:1349	arg1	responses					1322:1330	blunted responses	1314:1330	blunted responses of gene expression	1314:1349	In conclusion, despite differences in enzymatic regulations, and blunted responses of gene expression, high-sucrose diet was still able to induce a marked increase in liver lipid content in old animals.
29637446	9	13	theme	expression	1340:1349	arg1	differences					1272:1282	differences	1272:1282	differences in enzymatic regulations	1272:1307	In conclusion, despite differences in enzymatic regulations, and blunted responses of gene expression, high-sucrose diet was still able to induce a marked increase in liver lipid content in old animals.
29637446	5	14	theme	macronutrient	632:644	arg1	composition					646:656	liver macronutrient composition	626:656	liver macronutrient composition	626:656	We measured liver macronutrient composition, glycation/oxidative stress, enzyme activities (lipogenesis, β-oxidation, fructokinase), gene expression (enzymes and transcription factors), in vivo protein synthesis rates and plasma parameters.
29637446	4	15	theme	22-month-old	439:450	arg1	rats					457:460	22-month-old male rats	439:460	22-month-old male rats fed during 5 months with a diet containing either 13 or 62% sucrose, supplemented or not with rutin, vitamin E, A, D, selenium, and zinc	439:597	Four groups of 22-month-old male rats fed during 5 months with a diet containing either 13 or 62% sucrose, supplemented or not with rutin, vitamin E, A, D, selenium, and zinc were compared.
29637446	8	16	theme	oxidase	1197:1203	arg1	activities					1237:1246	fatty acid synthase, acyl coA oxidase, and possibly ATP-citrate lyase activities	1167:1246	fatty acid synthase, acyl coA oxidase, and possibly ATP-citrate lyase activities	1167:1246	Polyphenol/micronutrient supplementation tended to limit liver triglyceride infiltration through variations in fatty acid synthase, acyl coA oxidase, and possibly ATP-citrate lyase activities.
29637446	8	17	theme	Polyphenol/micronutrient	1056:1079	arg1	supplementation					1081:1095	Polyphenol/micronutrient supplementation	1056:1095	Polyphenol/micronutrient supplementation	1056:1095	Polyphenol/micronutrient supplementation tended to limit liver triglyceride infiltration through variations in fatty acid synthase, acyl coA oxidase, and possibly ATP-citrate lyase activities.
29637446	9	18	theme	marked	1397:1402	arg1	increase					1404:1411	a marked increase	1395:1411	a marked increase in liver lipid content in old animals	1395:1449	In conclusion, despite differences in enzymatic regulations, and blunted responses of gene expression, high-sucrose diet was still able to induce a marked increase in liver lipid content in old animals.
29637446	5	19	theme	gene	747:750	arg1	expression					752:761	gene expression	747:761	gene expression (enzymes and transcription factors)	747:797	We measured liver macronutrient composition, glycation/oxidative stress, enzyme activities (lipogenesis, β-oxidation, fructokinase), gene expression (enzymes and transcription factors), in vivo protein synthesis rates and plasma parameters.
29637446	2	20	from	liver	200:204	arg1	particular					186:195	particular	186:195	particular	186:195	High-sugar intake and senescence share common deleterious effects, in particular in liver, but combination of these two factors was little studied.
29637446	6	21	theme	liver	939:943	arg1	synthesis					953:961	liver protein synthesis	939:961	liver protein synthesis	939:961	Sucrose induced an increase in plasma and liver lipid content, and a stimulation of liver protein synthesis rates.
29637446	2	22	from	particular	186:195	arg1	liver					200:204	liver	200:204	liver	200:204	High-sugar intake and senescence share common deleterious effects, in particular in liver, but combination of these two factors was little studied.
29637446	4	23	theme	rats	457:460	arg1	rats					457:460	22-month-old male rats	439:460	22-month-old male rats fed during 5 months with a diet containing either 13 or 62% sucrose, supplemented or not with rutin, vitamin E, A, D, selenium, and zinc	439:597	Four groups of 22-month-old male rats fed during 5 months with a diet containing either 13 or 62% sucrose, supplemented or not with rutin, vitamin E, A, D, selenium, and zinc were compared.
29637446	4	23	theme	rats	457:460	arg1	groups					429:434	Four groups	424:434	Four groups of 22-month-old male rats fed during 5 months with a diet containing either 13 or 62% sucrose, supplemented or not with rutin, vitamin E, A, D, selenium, and zinc	424:597	Four groups of 22-month-old male rats fed during 5 months with a diet containing either 13 or 62% sucrose, supplemented or not with rutin, vitamin E, A, D, selenium, and zinc were compared.
29637446	8	24	theme	triglyceride	1119:1130	arg1	infiltration					1132:1143	liver triglyceride infiltration	1113:1143	liver triglyceride infiltration	1113:1143	Polyphenol/micronutrient supplementation tended to limit liver triglyceride infiltration through variations in fatty acid synthase, acyl coA oxidase, and possibly ATP-citrate lyase activities.
29637446	3	25	theme	polyphenol/micronutrient	382:405	arg1	supplementation					407:421	a polyphenol/micronutrient supplementation	380:421	a polyphenol/micronutrient supplementation	380:421	Our aims were to examine the effect of a high-sucrose diet in liver of old rats and also the potential benefices of a polyphenol/micronutrient supplementation.
29637446	3	26	theme	old	335:337	arg1	rats					339:342	old rats	335:342	old rats	335:342	Our aims were to examine the effect of a high-sucrose diet in liver of old rats and also the potential benefices of a polyphenol/micronutrient supplementation.
29637446	2	27	theme	High-sugar	116:125	arg1	intake					127:132	High-sugar intake	116:132	High-sugar intake	116:132	High-sugar intake and senescence share common deleterious effects, in particular in liver, but combination of these two factors was little studied.
29637446	5	28	dep	activities	694:703	arg1	lipogenesis					706:716	lipogenesis	706:716	lipogenesis	706:716	We measured liver macronutrient composition, glycation/oxidative stress, enzyme activities (lipogenesis, β-oxidation, fructokinase), gene expression (enzymes and transcription factors), in vivo protein synthesis rates and plasma parameters.
29637446	5	28	dep	activities	694:703	arg1	fructokinase					732:743	fructokinase	732:743	fructokinase	732:743	We measured liver macronutrient composition, glycation/oxidative stress, enzyme activities (lipogenesis, β-oxidation, fructokinase), gene expression (enzymes and transcription factors), in vivo protein synthesis rates and plasma parameters.
29637446	5	28	dep	activities	694:703	arg1	β-oxidation					719:729	β-oxidation	719:729	β-oxidation	719:729	We measured liver macronutrient composition, glycation/oxidative stress, enzyme activities (lipogenesis, β-oxidation, fructokinase), gene expression (enzymes and transcription factors), in vivo protein synthesis rates and plasma parameters.
29637446	6	29	from	stimulation	924:934	arg1	liver					897:901	liver	897:901	liver	897:901	Sucrose induced an increase in plasma and liver lipid content, and a stimulation of liver protein synthesis rates.
29637446	6	29	from	stimulation	924:934	arg1	plasma					886:891	plasma	886:891	plasma	886:891	Sucrose induced an increase in plasma and liver lipid content, and a stimulation of liver protein synthesis rates.
29637446	5	30	theme	plasma	836:841	arg1	parameters					843:852	plasma parameters	836:852	plasma parameters	836:852	We measured liver macronutrient composition, glycation/oxidative stress, enzyme activities (lipogenesis, β-oxidation, fructokinase), gene expression (enzymes and transcription factors), in vivo protein synthesis rates and plasma parameters.
29637446	5	31	theme	transcription	776:788	arg1	factors					790:796	transcription factors	776:796	transcription factors	776:796	We measured liver macronutrient composition, glycation/oxidative stress, enzyme activities (lipogenesis, β-oxidation, fructokinase), gene expression (enzymes and transcription factors), in vivo protein synthesis rates and plasma parameters.
29637446	3	32	theme	rats	339:342	arg1	liver					326:330	liver	326:330	liver of old rats	326:342	Our aims were to examine the effect of a high-sucrose diet in liver of old rats and also the potential benefices of a polyphenol/micronutrient supplementation.
29637446	5	33	dep	in	800:801	arg1	vivo					803:806	vivo	803:806	vivo	803:806	We measured liver macronutrient composition, glycation/oxidative stress, enzyme activities (lipogenesis, β-oxidation, fructokinase), gene expression (enzymes and transcription factors), in vivo protein synthesis rates and plasma parameters.
29637446	9	34	from	conclusion	1252:1261	arg1	able					1380:1383	able	1380:1383	able	1380:1383	In conclusion, despite differences in enzymatic regulations, and blunted responses of gene expression, high-sucrose diet was still able to induce a marked increase in liver lipid content in old animals.
29637446	8	35	theme	synthase	1178:1185	arg1	activities					1237:1246	fatty acid synthase, acyl coA oxidase, and possibly ATP-citrate lyase activities	1167:1246	fatty acid synthase, acyl coA oxidase, and possibly ATP-citrate lyase activities	1167:1246	Polyphenol/micronutrient supplementation tended to limit liver triglyceride infiltration through variations in fatty acid synthase, acyl coA oxidase, and possibly ATP-citrate lyase activities.
29637446	6	36	theme	synthesis	953:961	arg1	stimulation					924:934	a stimulation	922:934	a stimulation of liver protein synthesis	922:961	Sucrose induced an increase in plasma and liver lipid content, and a stimulation of liver protein synthesis rates.
29637446	6	36	theme	synthesis	953:961	arg1	increase					874:881	an increase	871:881	an increase in plasma and liver lipid content	871:915	Sucrose induced an increase in plasma and liver lipid content, and a stimulation of liver protein synthesis rates.
29637446	0	37	theme	chronic	15:21	arg1	intake					23:28	high chronic intake	10:28	high chronic intake of sucrose	10:39	Effect of high chronic intake of sucrose on liver metabolism in aging rats.
29637446	9	38	theme	high-sucrose	1352:1363	arg1	diet					1365:1368	high-sucrose diet	1352:1368	high-sucrose diet	1352:1368	In conclusion, despite differences in enzymatic regulations, and blunted responses of gene expression, high-sucrose diet was still able to induce a marked increase in liver lipid content in old animals.
29637446	6	39	theme	protein	945:951	arg1	synthesis					953:961	liver protein synthesis	939:961	liver protein synthesis	939:961	Sucrose induced an increase in plasma and liver lipid content, and a stimulation of liver protein synthesis rates.
29637446	0	40	theme	high	10:13	arg1	intake					23:28	high chronic intake	10:28	high chronic intake of sucrose	10:39	Effect of high chronic intake of sucrose on liver metabolism in aging rats.
29637446	7	41	theme	Gene	970:973	arg1	expression					975:984	Gene expression	970:984	Gene expression	970:984	Gene expression was little changed by sucrose, with lower levels for LXR-α and LXR-β.
29637446	4	42	theme	vitamin	563:569	arg1	5 months					473:480	5 months	473:480	5 months with a diet containing either 13 or 62% sucrose, supplemented or not with rutin	473:560	Four groups of 22-month-old male rats fed during 5 months with a diet containing either 13 or 62% sucrose, supplemented or not with rutin, vitamin E, A, D, selenium, and zinc were compared.
29637446	4	42	theme	vitamin	563:569	arg1	E					571:571	vitamin E	563:571	vitamin E	563:571	Four groups of 22-month-old male rats fed during 5 months with a diet containing either 13 or 62% sucrose, supplemented or not with rutin, vitamin E, A, D, selenium, and zinc were compared.
29637446	6	43	from	increase	874:881	arg1	liver					897:901	liver	897:901	liver	897:901	Sucrose induced an increase in plasma and liver lipid content, and a stimulation of liver protein synthesis rates.
29637446	6	43	from	increase	874:881	arg1	plasma					886:891	plasma	886:891	plasma	886:891	Sucrose induced an increase in plasma and liver lipid content, and a stimulation of liver protein synthesis rates.
29637446	4	44	with	5 months	473:480	arg1	diet					489:492	a diet	487:492	a diet containing either 13 or 62% sucrose	487:528	Four groups of 22-month-old male rats fed during 5 months with a diet containing either 13 or 62% sucrose, supplemented or not with rutin, vitamin E, A, D, selenium, and zinc were compared.
29637446	9	45	from	content	1428:1434	arg1	animals					1443:1449	old animals	1439:1449	old animals	1439:1449	In conclusion, despite differences in enzymatic regulations, and blunted responses of gene expression, high-sucrose diet was still able to induce a marked increase in liver lipid content in old animals.
29637446	2	46	theme	deleterious	162:172	arg1	effects					174:180	common deleterious effects	155:180	common deleterious effects	155:180	High-sugar intake and senescence share common deleterious effects, in particular in liver, but combination of these two factors was little studied.
29637446	8	47	theme	fatty	1167:1171	arg1	synthase					1178:1185	fatty acid synthase	1167:1185	fatty acid synthase	1167:1185	Polyphenol/micronutrient supplementation tended to limit liver triglyceride infiltration through variations in fatty acid synthase, acyl coA oxidase, and possibly ATP-citrate lyase activities.
29637446	3	48	theme	supplementation	407:421	arg1	benefices					367:375	the potential benefices	353:375	the potential benefices of a polyphenol/micronutrient supplementation	353:421	Our aims were to examine the effect of a high-sucrose diet in liver of old rats and also the potential benefices of a polyphenol/micronutrient supplementation.
29637446	0	49	theme	intake	23:28	arg1	Effect					0:5	Effect	0:5	Effect of high chronic intake of sucrose on liver metabolism in aging rats.	0:74	Effect of high chronic intake of sucrose on liver metabolism in aging rats.
29637446	3	50	theme	potential	357:365	arg1	benefices					367:375	the potential benefices	353:375	the potential benefices of a polyphenol/micronutrient supplementation	353:421	Our aims were to examine the effect of a high-sucrose diet in liver of old rats and also the potential benefices of a polyphenol/micronutrient supplementation.
29637446	2	51	theme	common	155:160	arg1	effects					174:180	common deleterious effects	155:180	common deleterious effects	155:180	High-sugar intake and senescence share common deleterious effects, in particular in liver, but combination of these two factors was little studied.
29637446	5	52	theme	synthesis	816:824	arg1	rates					826:830	in vivo protein synthesis rates	800:830	in vivo protein synthesis rates	800:830	We measured liver macronutrient composition, glycation/oxidative stress, enzyme activities (lipogenesis, β-oxidation, fructokinase), gene expression (enzymes and transcription factors), in vivo protein synthesis rates and plasma parameters.
29637446	3	53	theme	high-sucrose	305:316	arg1	diet					318:321	a high-sucrose diet	303:321	a high-sucrose diet	303:321	Our aims were to examine the effect of a high-sucrose diet in liver of old rats and also the potential benefices of a polyphenol/micronutrient supplementation.
29637446	7	54	theme	lower	1022:1026	arg1	levels					1028:1033	lower levels	1022:1033	lower levels for LXR-α and LXR-β	1022:1053	Gene expression was little changed by sucrose, with lower levels for LXR-α and LXR-β.
29637446	5	55	theme	liver	626:630	arg1	composition					646:656	liver macronutrient composition	626:656	liver macronutrient composition	626:656	We measured liver macronutrient composition, glycation/oxidative stress, enzyme activities (lipogenesis, β-oxidation, fructokinase), gene expression (enzymes and transcription factors), in vivo protein synthesis rates and plasma parameters.
29637446	8	56	theme	liver	1113:1117	arg1	infiltration					1132:1143	liver triglyceride infiltration	1113:1143	liver triglyceride infiltration	1113:1143	Polyphenol/micronutrient supplementation tended to limit liver triglyceride infiltration through variations in fatty acid synthase, acyl coA oxidase, and possibly ATP-citrate lyase activities.
29637446	4	57	dep	sucrose	522:528	arg1	%					520:520	%	520:520	%	520:520	Four groups of 22-month-old male rats fed during 5 months with a diet containing either 13 or 62% sucrose, supplemented or not with rutin, vitamin E, A, D, selenium, and zinc were compared.
29637446	5	58	theme	protein	808:814	arg1	rates					826:830	in vivo protein synthesis rates	800:830	in vivo protein synthesis rates	800:830	We measured liver macronutrient composition, glycation/oxidative stress, enzyme activities (lipogenesis, β-oxidation, fructokinase), gene expression (enzymes and transcription factors), in vivo protein synthesis rates and plasma parameters.
29637446	0	59	theme	sucrose	33:39	arg1	intake					23:28	high chronic intake	10:28	high chronic intake of sucrose	10:39	Effect of high chronic intake of sucrose on liver metabolism in aging rats.
29637446	9	60	theme	enzymatic	1287:1295	arg1	regulations					1297:1307	enzymatic regulations	1287:1307	enzymatic regulations	1287:1307	In conclusion, despite differences in enzymatic regulations, and blunted responses of gene expression, high-sucrose diet was still able to induce a marked increase in liver lipid content in old animals.
29637446	9	61	from	responses	1322:1330	arg1	regulations					1297:1307	enzymatic regulations	1287:1307	enzymatic regulations	1287:1307	In conclusion, despite differences in enzymatic regulations, and blunted responses of gene expression, high-sucrose diet was still able to induce a marked increase in liver lipid content in old animals.
29637446	9	62	from	able	1380:1383	arg1	conclusion					1252:1261	conclusion	1252:1261	conclusion	1252:1261	In conclusion, despite differences in enzymatic regulations, and blunted responses of gene expression, high-sucrose diet was still able to induce a marked increase in liver lipid content in old animals.
29637446	0	63	from	metabolism	50:59	arg1	rats					70:73	aging rats	64:73	aging rats	64:73	Effect of high chronic intake of sucrose on liver metabolism in aging rats.
29637446	9	64	from	increase	1404:1411	arg1	content					1428:1434	liver lipid content	1416:1434	liver lipid content in old animals	1416:1449	In conclusion, despite differences in enzymatic regulations, and blunted responses of gene expression, high-sucrose diet was still able to induce a marked increase in liver lipid content in old animals.
29637446	8	65	theme	coA	1193:1195	arg1	oxidase					1197:1203	acyl coA oxidase	1188:1203	acyl coA oxidase	1188:1203	Polyphenol/micronutrient supplementation tended to limit liver triglyceride infiltration through variations in fatty acid synthase, acyl coA oxidase, and possibly ATP-citrate lyase activities.
29637446	5	66	dep	expression	752:761	arg1	enzymes					764:770	enzymes	764:770	enzymes	764:770	We measured liver macronutrient composition, glycation/oxidative stress, enzyme activities (lipogenesis, β-oxidation, fructokinase), gene expression (enzymes and transcription factors), in vivo protein synthesis rates and plasma parameters.
29637446	5	66	dep	expression	752:761	arg1	factors					790:796	transcription factors	776:796	transcription factors	776:796	We measured liver macronutrient composition, glycation/oxidative stress, enzyme activities (lipogenesis, β-oxidation, fructokinase), gene expression (enzymes and transcription factors), in vivo protein synthesis rates and plasma parameters.
29637446	2	67	theme	factors	236:242	arg1	combination					211:221	combination	211:221	combination of these two factors	211:242	High-sugar intake and senescence share common deleterious effects, in particular in liver, but combination of these two factors was little studied.
29637446	8	68	theme	acid	1173:1176	arg1	synthase					1178:1185	fatty acid synthase	1167:1185	fatty acid synthase	1167:1185	Polyphenol/micronutrient supplementation tended to limit liver triglyceride infiltration through variations in fatty acid synthase, acyl coA oxidase, and possibly ATP-citrate lyase activities.
29637446	7	69	with	little	990:995	arg1	levels					1028:1033	lower levels	1022:1033	lower levels for LXR-α and LXR-β	1022:1053	Gene expression was little changed by sucrose, with lower levels for LXR-α and LXR-β.
29637446	8	70	theme	acyl	1188:1191	arg1	oxidase					1197:1203	acyl coA oxidase	1188:1203	acyl coA oxidase	1188:1203	Polyphenol/micronutrient supplementation tended to limit liver triglyceride infiltration through variations in fatty acid synthase, acyl coA oxidase, and possibly ATP-citrate lyase activities.
29637446	8	71	from	variations	1153:1162	arg1	activities					1237:1246	fatty acid synthase, acyl coA oxidase, and possibly ATP-citrate lyase activities	1167:1246	fatty acid synthase, acyl coA oxidase, and possibly ATP-citrate lyase activities	1167:1246	Polyphenol/micronutrient supplementation tended to limit liver triglyceride infiltration through variations in fatty acid synthase, acyl coA oxidase, and possibly ATP-citrate lyase activities.
29637446	3	72	from	effect	293:298	arg1	liver					326:330	liver	326:330	liver of old rats	326:342	Our aims were to examine the effect of a high-sucrose diet in liver of old rats and also the potential benefices of a polyphenol/micronutrient supplementation.
29637446	9	73	theme	liver	1416:1420	arg1	content					1428:1434	liver lipid content	1416:1434	liver lipid content in old animals	1416:1449	In conclusion, despite differences in enzymatic regulations, and blunted responses of gene expression, high-sucrose diet was still able to induce a marked increase in liver lipid content in old animals.
29637446	5	74	theme	glycation/oxidative	659:677	arg1	stress					679:684	glycation/oxidative stress	659:684	glycation/oxidative stress	659:684	We measured liver macronutrient composition, glycation/oxidative stress, enzyme activities (lipogenesis, β-oxidation, fructokinase), gene expression (enzymes and transcription factors), in vivo protein synthesis rates and plasma parameters.
29637446	6	75	dep	increase	874:881	arg1	rates					963:967	rates	963:967	rates	963:967	Sucrose induced an increase in plasma and liver lipid content, and a stimulation of liver protein synthesis rates.
29637446	9	76	theme	lipid	1422:1426	arg1	content					1428:1434	liver lipid content	1416:1434	liver lipid content in old animals	1416:1449	In conclusion, despite differences in enzymatic regulations, and blunted responses of gene expression, high-sucrose diet was still able to induce a marked increase in liver lipid content in old animals.
29637446	8	77	theme	lyase	1231:1235	arg1	activities					1237:1246	fatty acid synthase, acyl coA oxidase, and possibly ATP-citrate lyase activities	1167:1246	fatty acid synthase, acyl coA oxidase, and possibly ATP-citrate lyase activities	1167:1246	Polyphenol/micronutrient supplementation tended to limit liver triglyceride infiltration through variations in fatty acid synthase, acyl coA oxidase, and possibly ATP-citrate lyase activities.
29637446	9	78	theme	blunted	1314:1320	arg1	responses					1322:1330	blunted responses	1314:1330	blunted responses of gene expression	1314:1349	In conclusion, despite differences in enzymatic regulations, and blunted responses of gene expression, high-sucrose diet was still able to induce a marked increase in liver lipid content in old animals.
29637446	0	79	theme	liver	44:48	arg1	metabolism					50:59	liver metabolism	44:59	liver metabolism in aging rats	44:73	Effect of high chronic intake of sucrose on liver metabolism in aging rats.
29637446	10	80	theme	polyphenol/micronutrients	1507:1531	arg1	impact					1497:1502	the positive impact	1484:1502	the positive impact of polyphenol/micronutrients	1484:1531	However, it probably attenuated the positive impact of polyphenol/micronutrients.
29637446	10	81	theme	positive	1488:1495	arg1	impact					1497:1502	the positive impact	1484:1502	the positive impact of polyphenol/micronutrients	1484:1531	However, it probably attenuated the positive impact of polyphenol/micronutrients.
29637446	8	82	theme	ATP-citrate	1219:1229	arg1	lyase					1231:1235	ATP-citrate lyase	1219:1235	ATP-citrate lyase	1219:1235	Polyphenol/micronutrient supplementation tended to limit liver triglyceride infiltration through variations in fatty acid synthase, acyl coA oxidase, and possibly ATP-citrate lyase activities.
30856229	6	0	theme	pelleted	897:904	arg1	feed					906:909	captive Svalbard ptarmigans fed commercial pelleted feed	854:909	captive Svalbard ptarmigans fed commercial pelleted feed	854:909	Samples from captive Svalbard ptarmigans fed commercial pelleted feed were included to investigate the effect of diet on microbial composition and function.
30856229	4	1	theme	Arctic	551:556	arg1	Norway					558:563	Arctic Norway	551:563	Arctic Norway	551:563	The cecum microbiome in wild rock ptarmigans from Arctic Norway was characterized to unveil their functional potential for PSM detoxification, methanogenesis and polysaccharides degradation.
30856229	9	2	theme	compounds	1430:1438	arg1	conversion					1409:1418	the conversion	1405:1418	the conversion of phenol compounds to pyruvate	1405:1450	The ceca of wild ptarmigans housed bacterial groups involved in PSM-degradation, and genes mediating the conversion of phenol compounds to pyruvate.
30856229	6	3	theme	fed	882:884	arg1	feed					906:909	captive Svalbard ptarmigans fed commercial pelleted feed	854:909	captive Svalbard ptarmigans fed commercial pelleted feed	854:909	Samples from captive Svalbard ptarmigans fed commercial pelleted feed were included to investigate the effect of diet on microbial composition and function.
30856229	7	4	theme	functional	1107:1116	arg1	potential					1118:1126	functional potential	1107:1126	functional potential	1107:1126	Abundances of methanogens and bacteria were determined by qRT-PCR, while microbial community composition and functional potential were studied using 16S rRNA gene sequencing and shotgun metagenomics.
30856229	13	5	theme	wild	2010:2013	arg1	ptarmigans					2015:2024	wild ptarmigans	2010:2024	wild ptarmigans on mainland Norway and Svalbard	2010:2056	In conclusion, there were no physiological and phenotypical dissimilarities in the microbiota found in the cecum of wild ptarmigans on mainland Norway and Svalbard.
30856229	6	6	theme	Svalbard	862:869	arg1	feed					906:909	captive Svalbard ptarmigans fed commercial pelleted feed	854:909	captive Svalbard ptarmigans fed commercial pelleted feed	854:909	Samples from captive Svalbard ptarmigans fed commercial pelleted feed were included to investigate the effect of diet on microbial composition and function.
30856229	12	7	theme	genes	1796:1800	arg1	suite					1787:1791	a broad suite	1779:1791	a broad suite of genes for the depolymerization of hemicellulose and non-cellulosic polysaccharides (e.g. starch)	1779:1891	Both wild and captive ptarmigans possessed a broad suite of genes for the depolymerization of hemicellulose and non-cellulosic polysaccharides (e.g. starch).
30856229	14	8	theme	diets	2234:2238	arg1	differences					2077:2087	substantial differences	2065:2087	substantial differences in the functional potential for PSM degradation and methanogenesis in wild and captive birds	2065:2180	While substantial differences in the functional potential for PSM degradation and methanogenesis in wild and captive birds seem to be a direct consequence of their dissimilar diets.
30856229	14	8	theme	diets	2234:2238	arg1	consequence					2202:2212	a direct consequence	2193:2212	a direct consequence of their dissimilar diets	2193:2238	While substantial differences in the functional potential for PSM degradation and methanogenesis in wild and captive birds seem to be a direct consequence of their dissimilar diets.
30856229	2	9	theme	plant	258:262	arg1	parts					264:268	plant parts	258:268	plant parts	258:268	Their diet varies by season, including plants or plant parts of high nutritional value, but also toxic plant secondary metabolites (PSMs).
30856229	11	10	from	degradation	1646:1656	arg1	production					1623:1632	increased methanol production	1604:1632	increased methanol production from pectin degradation in wild birds due to a diet consisting of primarily fresh pectin-rich plants	1604:1733	It might be related to increased methanol production from pectin degradation in wild birds due to a diet consisting of primarily fresh pectin-rich plants.
30856229	8	11	theme	wild	1261:1264	arg1	ptarmigans					1266:1275	wild ptarmigans	1261:1275	wild ptarmigans	1261:1275	Abundances of bacteria and methanogenic Archaea were higher in wild ptarmigans compared to captive birds.
30856229	7	12	theme	microbial	1071:1079	arg1	composition					1091:1101	microbial community composition	1071:1101	microbial community composition	1071:1101	Abundances of methanogens and bacteria were determined by qRT-PCR, while microbial community composition and functional potential were studied using 16S rRNA gene sequencing and shotgun metagenomics.
30856229	2	13	theme	nutritional	278:288	arg1	value					290:294	high nutritional value	273:294	high nutritional value	273:294	Their diet varies by season, including plants or plant parts of high nutritional value, but also toxic plant secondary metabolites (PSMs).
30856229	8	14	theme	captive	1289:1295	arg1	birds					1297:1301	captive birds	1289:1301	captive birds	1289:1301	Abundances of bacteria and methanogenic Archaea were higher in wild ptarmigans compared to captive birds.
30856229	7	15	theme	gene	1156:1159	arg1	sequencing					1161:1170	16S rRNA gene sequencing	1147:1170	16S rRNA gene sequencing	1147:1170	Abundances of methanogens and bacteria were determined by qRT-PCR, while microbial community composition and functional potential were studied using 16S rRNA gene sequencing and shotgun metagenomics.
30856229	8	16	theme	Archaea	1238:1244	arg1	Abundances					1198:1207	Abundances	1198:1207	Abundances of bacteria and methanogenic Archaea	1198:1244	Abundances of bacteria and methanogenic Archaea were higher in wild ptarmigans compared to captive birds.
30856229	12	17	theme	wild	1741:1744	arg1	ptarmigans					1758:1767	Both wild and captive ptarmigans	1736:1767	Both wild and captive ptarmigans	1736:1767	Both wild and captive ptarmigans possessed a broad suite of genes for the depolymerization of hemicellulose and non-cellulosic polysaccharides (e.g. starch).
30856229	10	18	from	family	1501:1506	arg1	ptarmigans					1516:1525	wild ptarmigans	1511:1525	wild ptarmigans	1511:1525	Methanomassiliicoccaceae was the major archaeal family in wild ptarmigans, carrying the genes for methanogenesis from methanol.
30856229	5	19	theme	Cecal	692:696	arg1	samples					698:704	Cecal samples	692:704	Cecal samples	692:704	Cecal samples were collected from wild ptarmigans from Svalbard (L. m. hyperborea) and northern Norway (L. m. muta) during autumn/winter (Sept-Dec).
30856229	10	20	contain	carrying	1528:1535	arg2	genes					1541:1545	the genes	1537:1545	the genes for methanogenesis	1537:1564	Methanomassiliicoccaceae was the major archaeal family in wild ptarmigans, carrying the genes for methanogenesis from methanol.
30856229	10	20	contain	carrying	1528:1535	arg1	family					1501:1506	the major archaeal family	1482:1506	the major archaeal family	1482:1506	Methanomassiliicoccaceae was the major archaeal family in wild ptarmigans, carrying the genes for methanogenesis from methanol.
30856229	10	20	contain	carrying	1528:1535	arg1	Methanomassiliicoccaceae					1453:1476	Methanomassiliicoccaceae	1453:1476	Methanomassiliicoccaceae	1453:1476	Methanomassiliicoccaceae was the major archaeal family in wild ptarmigans, carrying the genes for methanogenesis from methanol.
30856229	12	21	theme	captive	1750:1756	arg1	ptarmigans					1758:1767	Both wild and captive ptarmigans	1736:1767	Both wild and captive ptarmigans	1736:1767	Both wild and captive ptarmigans possessed a broad suite of genes for the depolymerization of hemicellulose and non-cellulosic polysaccharides (e.g. starch).
30856229	0	22	theme	captive	55:61	arg1	ptarmigans					68:77	wild and captive rock ptarmigans	46:77	wild and captive rock ptarmigans indigenous to Arctic Norway	46:105	Characterization of the cecum microbiome from wild and captive rock ptarmigans indigenous to Arctic Norway.
30856229	13	23	from	ptarmigans	2015:2024	arg1	Norway					2038:2043	Norway	2038:2043	Norway	2038:2043	In conclusion, there were no physiological and phenotypical dissimilarities in the microbiota found in the cecum of wild ptarmigans on mainland Norway and Svalbard.
30856229	13	23	from	ptarmigans	2015:2024	arg1	Svalbard					2049:2056	Svalbard	2049:2056	Svalbard	2049:2056	In conclusion, there were no physiological and phenotypical dissimilarities in the microbiota found in the cecum of wild ptarmigans on mainland Norway and Svalbard.
30856229	1	24	theme	gallinaceous	143:154	arg1	birds					156:160	gallinaceous birds	143:160	gallinaceous birds inhabiting arctic and sub-arctic environments	143:206	Rock ptarmigans (Lagopus muta) are gallinaceous birds inhabiting arctic and sub-arctic environments.
30856229	1	24	theme	gallinaceous	143:154	arg1	ptarmigans					113:122	Rock ptarmigans	108:122	Rock ptarmigans (Lagopus muta)	108:137	Rock ptarmigans (Lagopus muta) are gallinaceous birds inhabiting arctic and sub-arctic environments.
30856229	14	25	theme	captive	2168:2174	arg1	birds					2176:2180	wild and captive birds	2159:2180	wild and captive birds	2159:2180	While substantial differences in the functional potential for PSM degradation and methanogenesis in wild and captive birds seem to be a direct consequence of their dissimilar diets.
30856229	4	26	from	Norway	558:563	arg1	microbiome					511:520	The cecum microbiome	501:520	The cecum microbiome in wild rock ptarmigans from Arctic Norway	501:563	The cecum microbiome in wild rock ptarmigans from Arctic Norway was characterized to unveil their functional potential for PSM detoxification, methanogenesis and polysaccharides degradation.
30856229	4	26	from	Norway	558:563	arg1	ptarmigans					535:544	wild rock ptarmigans	525:544	wild rock ptarmigans from Arctic Norway	525:563	The cecum microbiome in wild rock ptarmigans from Arctic Norway was characterized to unveil their functional potential for PSM detoxification, methanogenesis and polysaccharides degradation.
30856229	14	27	theme	wild	2159:2162	arg1	birds					2176:2180	wild and captive birds	2159:2180	wild and captive birds	2159:2180	While substantial differences in the functional potential for PSM degradation and methanogenesis in wild and captive birds seem to be a direct consequence of their dissimilar diets.
30856229	9	28	theme	ptarmigans	1321:1330	arg1	ceca					1308:1311	The ceca	1304:1311	The ceca of wild ptarmigans	1304:1330	The ceca of wild ptarmigans housed bacterial groups involved in PSM-degradation, and genes mediating the conversion of phenol compounds to pyruvate.
30856229	4	29	from	microbiome	511:520	arg1	Norway					558:563	Arctic Norway	551:563	Arctic Norway	551:563	The cecum microbiome in wild rock ptarmigans from Arctic Norway was characterized to unveil their functional potential for PSM detoxification, methanogenesis and polysaccharides degradation.
30856229	4	29	from	microbiome	511:520	arg1	ptarmigans					535:544	wild rock ptarmigans	525:544	wild rock ptarmigans from Arctic Norway	525:563	The cecum microbiome in wild rock ptarmigans from Arctic Norway was characterized to unveil their functional potential for PSM detoxification, methanogenesis and polysaccharides degradation.
30856229	10	30	theme	archaeal	1492:1499	arg1	family					1501:1506	the major archaeal family	1482:1506	the major archaeal family	1482:1506	Methanomassiliicoccaceae was the major archaeal family in wild ptarmigans, carrying the genes for methanogenesis from methanol.
30856229	10	30	theme	archaeal	1492:1499	arg1	Methanomassiliicoccaceae					1453:1476	Methanomassiliicoccaceae	1453:1476	Methanomassiliicoccaceae	1453:1476	Methanomassiliicoccaceae was the major archaeal family in wild ptarmigans, carrying the genes for methanogenesis from methanol.
30856229	3	31	theme	organic	391:397	arg1	decomposition					406:418	organic matter decomposition	391:418	organic matter decomposition in the cecum of ptarmigans	391:445	Little is known about the microbes driving organic matter decomposition in the cecum of ptarmigans, especially the last steps leading to methanogenesis.
30856229	7	32	theme	shotgun	1176:1182	arg1	metagenomics					1184:1195	shotgun metagenomics	1176:1195	shotgun metagenomics	1176:1195	Abundances of methanogens and bacteria were determined by qRT-PCR, while microbial community composition and functional potential were studied using 16S rRNA gene sequencing and shotgun metagenomics.
30856229	9	33	theme	bacterial	1339:1347	arg1	groups					1349:1354	bacterial groups	1339:1354	bacterial groups involved in PSM-degradation	1339:1382	The ceca of wild ptarmigans housed bacterial groups involved in PSM-degradation, and genes mediating the conversion of phenol compounds to pyruvate.
30856229	14	34	from	potential	2107:2115	arg1	birds					2176:2180	wild and captive birds	2159:2180	wild and captive birds	2159:2180	While substantial differences in the functional potential for PSM degradation and methanogenesis in wild and captive birds seem to be a direct consequence of their dissimilar diets.
30856229	6	35	from	effect	944:949	arg1	composition					972:982	microbial composition	962:982	microbial composition	962:982	Samples from captive Svalbard ptarmigans fed commercial pelleted feed were included to investigate the effect of diet on microbial composition and function.
30856229	6	35	from	effect	944:949	arg1	function					988:995	function	988:995	function	988:995	Samples from captive Svalbard ptarmigans fed commercial pelleted feed were included to investigate the effect of diet on microbial composition and function.
30856229	4	36	theme	rock	530:533	arg1	ptarmigans					535:544	wild rock ptarmigans	525:544	wild rock ptarmigans from Arctic Norway	525:563	The cecum microbiome in wild rock ptarmigans from Arctic Norway was characterized to unveil their functional potential for PSM detoxification, methanogenesis and polysaccharides degradation.
30856229	7	37	theme	rRNA	1151:1154	arg1	sequencing					1161:1170	16S rRNA gene sequencing	1147:1170	16S rRNA gene sequencing	1147:1170	Abundances of methanogens and bacteria were determined by qRT-PCR, while microbial community composition and functional potential were studied using 16S rRNA gene sequencing and shotgun metagenomics.
30856229	12	38	theme	non-cellulosic	1848:1861	arg1	polysaccharides					1863:1877	hemicellulose and non-cellulosic polysaccharides	1830:1877	hemicellulose and non-cellulosic polysaccharides (e.g. starch)	1830:1891	Both wild and captive ptarmigans possessed a broad suite of genes for the depolymerization of hemicellulose and non-cellulosic polysaccharides (e.g. starch).
30856229	14	39	theme	functional	2096:2105	arg1	potential					2107:2115	the functional potential	2092:2115	the functional potential for PSM degradation and methanogenesis in wild and captive birds	2092:2180	While substantial differences in the functional potential for PSM degradation and methanogenesis in wild and captive birds seem to be a direct consequence of their dissimilar diets.
30856229	11	40	theme	pectin-rich	1716:1726	arg1	plants					1728:1733	primarily fresh pectin-rich plants	1700:1733	primarily fresh pectin-rich plants	1700:1733	It might be related to increased methanol production from pectin degradation in wild birds due to a diet consisting of primarily fresh pectin-rich plants.
30856229	2	41	theme	secondary	318:326	arg1	PSMs					341:344	PSMs	341:344	PSMs	341:344	Their diet varies by season, including plants or plant parts of high nutritional value, but also toxic plant secondary metabolites (PSMs).
30856229	2	41	theme	secondary	318:326	arg1	metabolites					328:338	toxic plant secondary metabolites	306:338	toxic plant secondary metabolites (PSMs)	306:345	Their diet varies by season, including plants or plant parts of high nutritional value, but also toxic plant secondary metabolites (PSMs).
30856229	0	42	theme	wild	46:49	arg1	ptarmigans					68:77	wild and captive rock ptarmigans	46:77	wild and captive rock ptarmigans indigenous to Arctic Norway	46:105	Characterization of the cecum microbiome from wild and captive rock ptarmigans indigenous to Arctic Norway.
30856229	4	43	theme	cecum	505:509	arg1	microbiome					511:520	The cecum microbiome	501:520	The cecum microbiome in wild rock ptarmigans from Arctic Norway	501:563	The cecum microbiome in wild rock ptarmigans from Arctic Norway was characterized to unveil their functional potential for PSM detoxification, methanogenesis and polysaccharides degradation.
30856229	11	44	theme	methanol	1614:1621	arg1	production					1623:1632	increased methanol production	1604:1632	increased methanol production from pectin degradation in wild birds due to a diet consisting of primarily fresh pectin-rich plants	1604:1733	It might be related to increased methanol production from pectin degradation in wild birds due to a diet consisting of primarily fresh pectin-rich plants.
30856229	2	45	theme	toxic	306:310	arg1	PSMs					341:344	PSMs	341:344	PSMs	341:344	Their diet varies by season, including plants or plant parts of high nutritional value, but also toxic plant secondary metabolites (PSMs).
30856229	2	45	theme	toxic	306:310	arg1	metabolites					328:338	toxic plant secondary metabolites	306:338	toxic plant secondary metabolites (PSMs)	306:345	Their diet varies by season, including plants or plant parts of high nutritional value, but also toxic plant secondary metabolites (PSMs).
30856229	11	46	theme	wild	1661:1664	arg1	birds					1666:1670	wild birds	1661:1670	wild birds	1661:1670	It might be related to increased methanol production from pectin degradation in wild birds due to a diet consisting of primarily fresh pectin-rich plants.
30856229	4	47	dep	detoxification	628:641	arg1	degradation					679:689	degradation	679:689	degradation	679:689	The cecum microbiome in wild rock ptarmigans from Arctic Norway was characterized to unveil their functional potential for PSM detoxification, methanogenesis and polysaccharides degradation.
30856229	12	48	theme	hemicellulose	1830:1842	arg1	polysaccharides					1863:1877	hemicellulose and non-cellulosic polysaccharides	1830:1877	hemicellulose and non-cellulosic polysaccharides (e.g. starch)	1830:1891	Both wild and captive ptarmigans possessed a broad suite of genes for the depolymerization of hemicellulose and non-cellulosic polysaccharides (e.g. starch).
30856229	6	49	from	feed	906:909	arg1	Samples					841:847	Samples	841:847	Samples from captive Svalbard ptarmigans fed commercial pelleted feed	841:909	Samples from captive Svalbard ptarmigans fed commercial pelleted feed were included to investigate the effect of diet on microbial composition and function.
30856229	9	50	theme	phenol	1423:1428	arg1	compounds					1430:1438	phenol compounds	1423:1438	phenol compounds	1423:1438	The ceca of wild ptarmigans housed bacterial groups involved in PSM-degradation, and genes mediating the conversion of phenol compounds to pyruvate.
30856229	6	51	theme	commercial	886:895	arg1	feed					906:909	captive Svalbard ptarmigans fed commercial pelleted feed	854:909	captive Svalbard ptarmigans fed commercial pelleted feed	854:909	Samples from captive Svalbard ptarmigans fed commercial pelleted feed were included to investigate the effect of diet on microbial composition and function.
30856229	2	52	theme	high	273:276	arg1	value					290:294	high nutritional value	273:294	high nutritional value	273:294	Their diet varies by season, including plants or plant parts of high nutritional value, but also toxic plant secondary metabolites (PSMs).
30856229	6	53	theme	ptarmigans	871:880	arg1	feed					906:909	captive Svalbard ptarmigans fed commercial pelleted feed	854:909	captive Svalbard ptarmigans fed commercial pelleted feed	854:909	Samples from captive Svalbard ptarmigans fed commercial pelleted feed were included to investigate the effect of diet on microbial composition and function.
30856229	5	54	dep	L.	796:797	arg1	muta					802:805	L. m. muta	796:805	L. m. muta	796:805	Cecal samples were collected from wild ptarmigans from Svalbard (L. m. hyperborea) and northern Norway (L. m. muta) during autumn/winter (Sept-Dec).
30856229	7	55	theme	community	1081:1089	arg1	composition					1091:1101	microbial community composition	1071:1101	microbial community composition	1071:1101	Abundances of methanogens and bacteria were determined by qRT-PCR, while microbial community composition and functional potential were studied using 16S rRNA gene sequencing and shotgun metagenomics.
30856229	13	56	theme	ptarmigans	2015:2024	arg1	cecum					2001:2005	the cecum	1997:2005	the cecum of wild ptarmigans on mainland Norway and Svalbard	1997:2056	In conclusion, there were no physiological and phenotypical dissimilarities in the microbiota found in the cecum of wild ptarmigans on mainland Norway and Svalbard.
30856229	13	57	from	Norway	2038:2043	arg1	cecum					2001:2005	the cecum	1997:2005	the cecum of wild ptarmigans on mainland Norway and Svalbard	1997:2056	In conclusion, there were no physiological and phenotypical dissimilarities in the microbiota found in the cecum of wild ptarmigans on mainland Norway and Svalbard.
30856229	6	58	theme	captive	854:860	arg1	feed					906:909	captive Svalbard ptarmigans fed commercial pelleted feed	854:909	captive Svalbard ptarmigans fed commercial pelleted feed	854:909	Samples from captive Svalbard ptarmigans fed commercial pelleted feed were included to investigate the effect of diet on microbial composition and function.
30856229	14	59	theme	dissimilar	2223:2232	arg1	diets					2234:2238	their dissimilar diets	2217:2238	their dissimilar diets	2217:2238	While substantial differences in the functional potential for PSM degradation and methanogenesis in wild and captive birds seem to be a direct consequence of their dissimilar diets.
30856229	1	60	theme	arctic	173:178	arg1	environments					195:206	arctic and sub-arctic environments	173:206	arctic and sub-arctic environments	173:206	Rock ptarmigans (Lagopus muta) are gallinaceous birds inhabiting arctic and sub-arctic environments.
30856229	6	61	theme	diet	954:957	arg1	effect					944:949	the effect	940:949	the effect of diet on microbial composition and function	940:995	Samples from captive Svalbard ptarmigans fed commercial pelleted feed were included to investigate the effect of diet on microbial composition and function.
30856229	12	62	theme	broad	1781:1785	arg1	suite					1787:1791	a broad suite	1779:1791	a broad suite of genes for the depolymerization of hemicellulose and non-cellulosic polysaccharides (e.g. starch)	1779:1891	Both wild and captive ptarmigans possessed a broad suite of genes for the depolymerization of hemicellulose and non-cellulosic polysaccharides (e.g. starch).
30856229	2	63	theme	value	290:294	arg1	PSMs					341:344	PSMs	341:344	PSMs	341:344	Their diet varies by season, including plants or plant parts of high nutritional value, but also toxic plant secondary metabolites (PSMs).
30856229	2	63	theme	value	290:294	arg1	plants					248:253	plants	248:253	plants	248:253	Their diet varies by season, including plants or plant parts of high nutritional value, but also toxic plant secondary metabolites (PSMs).
30856229	2	63	theme	value	290:294	arg1	parts					264:268	plant parts	258:268	plant parts	258:268	Their diet varies by season, including plants or plant parts of high nutritional value, but also toxic plant secondary metabolites (PSMs).
30856229	2	63	theme	value	290:294	arg1	metabolites					328:338	toxic plant secondary metabolites	306:338	toxic plant secondary metabolites (PSMs)	306:345	Their diet varies by season, including plants or plant parts of high nutritional value, but also toxic plant secondary metabolites (PSMs).
30856229	1	64	theme	sub-arctic	184:193	arg1	environments					195:206	arctic and sub-arctic environments	173:206	arctic and sub-arctic environments	173:206	Rock ptarmigans (Lagopus muta) are gallinaceous birds inhabiting arctic and sub-arctic environments.
30856229	4	65	theme	functional	599:608	arg1	potential					610:618	their functional potential	593:618	their functional potential for PSM detoxification, methanogenesis and polysaccharides degradation	593:689	The cecum microbiome in wild rock ptarmigans from Arctic Norway was characterized to unveil their functional potential for PSM detoxification, methanogenesis and polysaccharides degradation.
30856229	14	66	theme	direct	2195:2200	arg1	differences					2077:2087	substantial differences	2065:2087	substantial differences in the functional potential for PSM degradation and methanogenesis in wild and captive birds	2065:2180	While substantial differences in the functional potential for PSM degradation and methanogenesis in wild and captive birds seem to be a direct consequence of their dissimilar diets.
30856229	14	66	theme	direct	2195:2200	arg1	consequence					2202:2212	a direct consequence	2193:2212	a direct consequence of their dissimilar diets	2193:2238	While substantial differences in the functional potential for PSM degradation and methanogenesis in wild and captive birds seem to be a direct consequence of their dissimilar diets.
30856229	2	67	theme	plant	312:316	arg1	PSMs					341:344	PSMs	341:344	PSMs	341:344	Their diet varies by season, including plants or plant parts of high nutritional value, but also toxic plant secondary metabolites (PSMs).
30856229	2	67	theme	plant	312:316	arg1	metabolites					328:338	toxic plant secondary metabolites	306:338	toxic plant secondary metabolites (PSMs)	306:345	Their diet varies by season, including plants or plant parts of high nutritional value, but also toxic plant secondary metabolites (PSMs).
30856229	11	68	from	production	1623:1632	arg1	birds					1666:1670	wild birds	1661:1670	wild birds	1661:1670	It might be related to increased methanol production from pectin degradation in wild birds due to a diet consisting of primarily fresh pectin-rich plants.
30856229	8	69	theme	bacteria	1212:1219	arg1	Abundances					1198:1207	Abundances	1198:1207	Abundances of bacteria and methanogenic Archaea	1198:1244	Abundances of bacteria and methanogenic Archaea were higher in wild ptarmigans compared to captive birds.
30856229	5	70	theme	wild	726:729	arg1	ptarmigans					731:740	wild ptarmigans	726:740	wild ptarmigans	726:740	Cecal samples were collected from wild ptarmigans from Svalbard (L. m. hyperborea) and northern Norway (L. m. muta) during autumn/winter (Sept-Dec).
30856229	7	71	theme	bacteria	1028:1035	arg1	Abundances					998:1007	Abundances	998:1007	Abundances of methanogens and bacteria	998:1035	Abundances of methanogens and bacteria were determined by qRT-PCR, while microbial community composition and functional potential were studied using 16S rRNA gene sequencing and shotgun metagenomics.
30856229	1	72	theme	Rock	108:111	arg1	birds					156:160	gallinaceous birds	143:160	gallinaceous birds inhabiting arctic and sub-arctic environments	143:206	Rock ptarmigans (Lagopus muta) are gallinaceous birds inhabiting arctic and sub-arctic environments.
30856229	1	72	theme	Rock	108:111	arg1	ptarmigans					113:122	Rock ptarmigans	108:122	Rock ptarmigans (Lagopus muta)	108:137	Rock ptarmigans (Lagopus muta) are gallinaceous birds inhabiting arctic and sub-arctic environments.
30856229	8	73	theme	methanogenic	1225:1236	arg1	Archaea					1238:1244	methanogenic Archaea	1225:1244	methanogenic Archaea	1225:1244	Abundances of bacteria and methanogenic Archaea were higher in wild ptarmigans compared to captive birds.
30856229	5	74	dep	Svalbard	747:754	arg1	hyperborea					763:772	L. m. hyperborea	757:772	L. m. hyperborea	757:772	Cecal samples were collected from wild ptarmigans from Svalbard (L. m. hyperborea) and northern Norway (L. m. muta) during autumn/winter (Sept-Dec).
30856229	13	75	theme	phenotypical	1941:1952	arg1	dissimilarities					1954:1968	no physiological and phenotypical dissimilarities	1920:1968	no physiological and phenotypical dissimilarities	1920:1968	In conclusion, there were no physiological and phenotypical dissimilarities in the microbiota found in the cecum of wild ptarmigans on mainland Norway and Svalbard.
30856229	7	76	theme	methanogens	1012:1022	arg1	Abundances					998:1007	Abundances	998:1007	Abundances of methanogens and bacteria	998:1035	Abundances of methanogens and bacteria were determined by qRT-PCR, while microbial community composition and functional potential were studied using 16S rRNA gene sequencing and shotgun metagenomics.
30856229	0	77	theme	rock	63:66	arg1	ptarmigans					68:77	wild and captive rock ptarmigans	46:77	wild and captive rock ptarmigans indigenous to Arctic Norway	46:105	Characterization of the cecum microbiome from wild and captive rock ptarmigans indigenous to Arctic Norway.
30856229	12	78	contain	possessed	1769:1777	arg1	ptarmigans					1758:1767	Both wild and captive ptarmigans	1736:1767	Both wild and captive ptarmigans	1736:1767	Both wild and captive ptarmigans possessed a broad suite of genes for the depolymerization of hemicellulose and non-cellulosic polysaccharides (e.g. starch).
30856229	12	78	contain	possessed	1769:1777	arg2	suite					1787:1791	a broad suite	1779:1791	a broad suite of genes for the depolymerization of hemicellulose and non-cellulosic polysaccharides (e.g. starch)	1779:1891	Both wild and captive ptarmigans possessed a broad suite of genes for the depolymerization of hemicellulose and non-cellulosic polysaccharides (e.g. starch).
30856229	0	79	theme	indigenous	79:88	arg1	ptarmigans					68:77	wild and captive rock ptarmigans	46:77	wild and captive rock ptarmigans indigenous to Arctic Norway	46:105	Characterization of the cecum microbiome from wild and captive rock ptarmigans indigenous to Arctic Norway.
30856229	13	80	from	cecum	2001:2005	arg1	Norway					2038:2043	Norway	2038:2043	Norway	2038:2043	In conclusion, there were no physiological and phenotypical dissimilarities in the microbiota found in the cecum of wild ptarmigans on mainland Norway and Svalbard.
30856229	13	80	from	cecum	2001:2005	arg1	Svalbard					2049:2056	Svalbard	2049:2056	Svalbard	2049:2056	In conclusion, there were no physiological and phenotypical dissimilarities in the microbiota found in the cecum of wild ptarmigans on mainland Norway and Svalbard.
30856229	13	81	located	found	1988:1992	arg2	microbiota					1977:1986	the microbiota	1973:1986	the microbiota found in the cecum of wild ptarmigans on mainland Norway and Svalbard	1973:2056	In conclusion, there were no physiological and phenotypical dissimilarities in the microbiota found in the cecum of wild ptarmigans on mainland Norway and Svalbard.
30856229	13	81	located	found	1988:1992	arg1	cecum					2001:2005	the cecum	1997:2005	the cecum of wild ptarmigans on mainland Norway and Svalbard	1997:2056	In conclusion, there were no physiological and phenotypical dissimilarities in the microbiota found in the cecum of wild ptarmigans on mainland Norway and Svalbard.
30856229	13	82	theme	physiological	1923:1935	arg1	dissimilarities					1954:1968	no physiological and phenotypical dissimilarities	1920:1968	no physiological and phenotypical dissimilarities	1920:1968	In conclusion, there were no physiological and phenotypical dissimilarities in the microbiota found in the cecum of wild ptarmigans on mainland Norway and Svalbard.
30856229	0	83	theme	Arctic	93:98	arg1	Norway					100:105	Arctic Norway	93:105	Arctic Norway	93:105	Characterization of the cecum microbiome from wild and captive rock ptarmigans indigenous to Arctic Norway.
30856229	14	84	theme	substantial	2065:2075	arg1	differences					2077:2087	substantial differences	2065:2087	substantial differences in the functional potential for PSM degradation and methanogenesis in wild and captive birds	2065:2180	While substantial differences in the functional potential for PSM degradation and methanogenesis in wild and captive birds seem to be a direct consequence of their dissimilar diets.
30856229	14	84	theme	substantial	2065:2075	arg1	consequence					2202:2212	a direct consequence	2193:2212	a direct consequence of their dissimilar diets	2193:2238	While substantial differences in the functional potential for PSM degradation and methanogenesis in wild and captive birds seem to be a direct consequence of their dissimilar diets.
30856229	6	85	theme	microbial	962:970	arg1	composition					972:982	microbial composition	962:982	microbial composition	962:982	Samples from captive Svalbard ptarmigans fed commercial pelleted feed were included to investigate the effect of diet on microbial composition and function.
30856229	9	86	theme	wild	1316:1319	arg1	ptarmigans					1321:1330	wild ptarmigans	1316:1330	wild ptarmigans	1316:1330	The ceca of wild ptarmigans housed bacterial groups involved in PSM-degradation, and genes mediating the conversion of phenol compounds to pyruvate.
30856229	1	87	dep	ptarmigans	113:122	arg1	muta					133:136	Lagopus muta	125:136	Lagopus muta	125:136	Rock ptarmigans (Lagopus muta) are gallinaceous birds inhabiting arctic and sub-arctic environments.
30856229	13	88	from	Svalbard	2049:2056	arg1	cecum					2001:2005	the cecum	1997:2005	the cecum of wild ptarmigans on mainland Norway and Svalbard	1997:2056	In conclusion, there were no physiological and phenotypical dissimilarities in the microbiota found in the cecum of wild ptarmigans on mainland Norway and Svalbard.
30856229	4	89	theme	wild	525:528	arg1	ptarmigans					535:544	wild rock ptarmigans	525:544	wild rock ptarmigans from Arctic Norway	525:563	The cecum microbiome in wild rock ptarmigans from Arctic Norway was characterized to unveil their functional potential for PSM detoxification, methanogenesis and polysaccharides degradation.
30856229	10	90	theme	major	1486:1490	arg1	family					1501:1506	the major archaeal family	1482:1506	the major archaeal family	1482:1506	Methanomassiliicoccaceae was the major archaeal family in wild ptarmigans, carrying the genes for methanogenesis from methanol.
30856229	10	90	theme	major	1486:1490	arg1	Methanomassiliicoccaceae					1453:1476	Methanomassiliicoccaceae	1453:1476	Methanomassiliicoccaceae	1453:1476	Methanomassiliicoccaceae was the major archaeal family in wild ptarmigans, carrying the genes for methanogenesis from methanol.
30856229	3	91	theme	matter	399:404	arg1	decomposition					406:418	organic matter decomposition	391:418	organic matter decomposition in the cecum of ptarmigans	391:445	Little is known about the microbes driving organic matter decomposition in the cecum of ptarmigans, especially the last steps leading to methanogenesis.
30856229	0	92	theme	cecum	24:28	arg1	Characterization					0:15	Characterization	0:15	Characterization of the cecum	0:28	Characterization of the cecum microbiome from wild and captive rock ptarmigans indigenous to Arctic Norway.
30856229	14	93	theme	PSM	2121:2123	arg1	degradation					2125:2135	PSM degradation	2121:2135	PSM degradation	2121:2135	While substantial differences in the functional potential for PSM degradation and methanogenesis in wild and captive birds seem to be a direct consequence of their dissimilar diets.
30856229	3	94	theme	ptarmigans	436:445	arg1	cecum					427:431	the cecum	423:431	the cecum of ptarmigans	423:445	Little is known about the microbes driving organic matter decomposition in the cecum of ptarmigans, especially the last steps leading to methanogenesis.
30856229	7	95	theme	16S	1147:1149	arg1	sequencing					1161:1170	16S rRNA gene sequencing	1147:1170	16S rRNA gene sequencing	1147:1170	Abundances of methanogens and bacteria were determined by qRT-PCR, while microbial community composition and functional potential were studied using 16S rRNA gene sequencing and shotgun metagenomics.
30856229	4	96	theme	PSM	624:626	arg1	detoxification					628:641	PSM detoxification	624:641	PSM detoxification	624:641	The cecum microbiome in wild rock ptarmigans from Arctic Norway was characterized to unveil their functional potential for PSM detoxification, methanogenesis and polysaccharides degradation.
30856229	10	97	theme	wild	1511:1514	arg1	ptarmigans					1516:1525	wild ptarmigans	1511:1525	wild ptarmigans	1511:1525	Methanomassiliicoccaceae was the major archaeal family in wild ptarmigans, carrying the genes for methanogenesis from methanol.
30856229	11	98	theme	fresh	1710:1714	arg1	plants					1728:1733	primarily fresh pectin-rich plants	1700:1733	primarily fresh pectin-rich plants	1700:1733	It might be related to increased methanol production from pectin degradation in wild birds due to a diet consisting of primarily fresh pectin-rich plants.
30856229	12	99	dep	starch	1885:1890	arg1	e.g.					1880:1883	e.g.	1880:1883	e.g.	1880:1883	Both wild and captive ptarmigans possessed a broad suite of genes for the depolymerization of hemicellulose and non-cellulosic polysaccharides (e.g. starch).
30856229	11	100	theme	increased	1604:1612	arg1	production					1623:1632	increased methanol production	1604:1632	increased methanol production from pectin degradation in wild birds due to a diet consisting of primarily fresh pectin-rich plants	1604:1733	It might be related to increased methanol production from pectin degradation in wild birds due to a diet consisting of primarily fresh pectin-rich plants.
30856229	3	101	theme	last	463:466	arg1	microbes					374:381	the microbes	370:381	the microbes driving organic matter decomposition in the cecum of ptarmigans	370:445	Little is known about the microbes driving organic matter decomposition in the cecum of ptarmigans, especially the last steps leading to methanogenesis.
30856229	3	101	theme	last	463:466	arg1	steps					468:472	the last steps	459:472	the last steps leading to methanogenesis	459:498	Little is known about the microbes driving organic matter decomposition in the cecum of ptarmigans, especially the last steps leading to methanogenesis.
30856229	14	102	from	differences	2077:2087	arg1	potential					2107:2115	the functional potential	2092:2115	the functional potential for PSM degradation and methanogenesis in wild and captive birds	2092:2180	While substantial differences in the functional potential for PSM degradation and methanogenesis in wild and captive birds seem to be a direct consequence of their dissimilar diets.
30856229	12	103	theme	polysaccharides	1863:1877	arg1	depolymerization					1810:1825	the depolymerization	1806:1825	the depolymerization of hemicellulose and non-cellulosic polysaccharides (e.g. starch)	1806:1891	Both wild and captive ptarmigans possessed a broad suite of genes for the depolymerization of hemicellulose and non-cellulosic polysaccharides (e.g. starch).
30856229	3	104	from	decomposition	406:418	arg1	cecum					427:431	the cecum	423:431	the cecum of ptarmigans	423:445	Little is known about the microbes driving organic matter decomposition in the cecum of ptarmigans, especially the last steps leading to methanogenesis.
30856229	11	105	theme	pectin	1639:1644	arg1	degradation					1646:1656	pectin degradation	1639:1656	pectin degradation	1639:1656	It might be related to increased methanol production from pectin degradation in wild birds due to a diet consisting of primarily fresh pectin-rich plants.
30418545	12	0	theme	Better	1854:1859	arg1	understanding					1861:1873	Better understanding	1854:1873	Better understanding of the role of succinate in infants and its potential effects on microbiome or mucosal immunity	1854:1969	Better understanding of the role of succinate in infants and its potential effects on microbiome or mucosal immunity merits further investigations.
30418545	9	1	theme	milk	1445:1448	arg1	profile					1476:1482	the milk cytokines and metabolites profile	1441:1482	the milk cytokines and metabolites profile	1441:1482	In addition, we noticed that the type of treatment [5-aminosalicylic acid versus biologics] influenced the milk cytokines and metabolites profile.
30418545	11	2	from	impact	1824:1829	arg1	microbiome					1842:1851	the gut microbiome	1834:1851	the gut microbiome	1834:1851	On the other hand, presence of higher levels of pro-inflammatory cytokines, lactate, and succinate may predispose the offspring to an inflammatory condition or impact on the gut microbiome.
30418545	2	3	theme	breast	509:514	arg1	milk					516:519	IBD breast milk	505:519	IBD breast milk	505:519	We aimed to investigate whether breast milk composition differs in healthy control [HC] versus IBD mothers in terms of antibodies, cytokines, and metabolite,s to identify potential impact of IBD breast milk on neonatal immune system.
30418545	10	4	from	reduction	1501:1509	arg1	sIgA					1569:1572	sIgA	1569:1572	sIgA	1569:1572	CONCLUSIONS The reduction in immunoprotective components of IBD breast milk such as sIgA and lactose theoretically may modulate the potential protective effects of breastfeeding.
30418545	10	4	from	reduction	1501:1509	arg1	components					1531:1540	immunoprotective components	1514:1540	immunoprotective components of IBD breast milk such as sIgA and lactose	1514:1584	CONCLUSIONS The reduction in immunoprotective components of IBD breast milk such as sIgA and lactose theoretically may modulate the potential protective effects of breastfeeding.
30418545	10	4	from	reduction	1501:1509	arg1	lactose					1578:1584	lactose	1578:1584	lactose	1578:1584	CONCLUSIONS The reduction in immunoprotective components of IBD breast milk such as sIgA and lactose theoretically may modulate the potential protective effects of breastfeeding.
30418545	0	5	from	Impact	133:138	arg1	Newborn					147:153	Newborn	147:153	Newborn	147:153	The Profile of Human Milk Metabolome, Cytokines, and Antibodies in Inflammatory Bowel Diseases Versus Healthy Mothers, and Potential Impact on the Newborn.
30418545	0	5	from	Impact	133:138	arg1	Diseases					86:93	Inflammatory Bowel Diseases	67:93	Inflammatory Bowel Diseases	67:93	The Profile of Human Milk Metabolome, Cytokines, and Antibodies in Inflammatory Bowel Diseases Versus Healthy Mothers, and Potential Impact on the Newborn.
30418545	0	5	from	Impact	133:138	arg1	Mothers					110:116	Healthy Mothers	102:116	Healthy Mothers	102:116	The Profile of Human Milk Metabolome, Cytokines, and Antibodies in Inflammatory Bowel Diseases Versus Healthy Mothers, and Potential Impact on the Newborn.
30418545	12	6	theme	succinate	1890:1898	arg1	role					1882:1885	the role	1878:1885	the role of succinate in infants	1878:1909	Better understanding of the role of succinate in infants and its potential effects on microbiome or mucosal immunity merits further investigations.
30418545	12	6	theme	succinate	1890:1898	arg1	effects					1929:1935	its potential effects	1915:1935	its potential effects on microbiome or mucosal immunity	1915:1969	Better understanding of the role of succinate in infants and its potential effects on microbiome or mucosal immunity merits further investigations.
30418545	2	7	theme	immune	533:538	arg1	system					540:545	neonatal immune system	524:545	neonatal immune system	524:545	We aimed to investigate whether breast milk composition differs in healthy control [HC] versus IBD mothers in terms of antibodies, cytokines, and metabolite,s to identify potential impact of IBD breast milk on neonatal immune system.
30418545	6	8	theme	resonance	978:986	arg1	[NMR					988:991	1H nuclear magnetic resonance [NMR	958:991	1H nuclear magnetic resonance [NMR	958:991	Moreover, breast milk metabolites were analysed by 1H nuclear magnetic resonance [NMR].
30418545	11	9	from	condition	1811:1819	arg1	microbiome					1842:1851	the gut microbiome	1834:1851	the gut microbiome	1834:1851	On the other hand, presence of higher levels of pro-inflammatory cytokines, lactate, and succinate may predispose the offspring to an inflammatory condition or impact on the gut microbiome.
30418545	12	10	theme	mucosal	1954:1960	arg1	immunity					1962:1969	mucosal immunity	1954:1969	mucosal immunity	1954:1969	Better understanding of the role of succinate in infants and its potential effects on microbiome or mucosal immunity merits further investigations.
30418545	3	11	theme	disease	623:629	arg1	[CD					631:633	Crohn's disease [CD]	615:634	Crohn's disease [CD]	615:634	METHODS Breast milk specimens from HC [n = 17] and IBD [n = 31 for Crohn's disease [CD]; and n = 41 for ulcerative colitis [UC]; were collected at 3 and 6 months postpartum [PP3] and [PP6], respectively.
30418545	9	12	theme	cytokines	1450:1458	arg1	profile					1476:1482	the milk cytokines and metabolites profile	1441:1482	the milk cytokines and metabolites profile	1441:1482	In addition, we noticed that the type of treatment [5-aminosalicylic acid versus biologics] influenced the milk cytokines and metabolites profile.
30418545	6	13	theme	nuclear	961:967	arg1	resonance					978:986	1H nuclear magnetic resonance	958:986	1H nuclear magnetic resonance [NMR	958:991	Moreover, breast milk metabolites were analysed by 1H nuclear magnetic resonance [NMR].
30418545	0	14	theme	Healthy	102:108	arg1	Mothers					110:116	Healthy Mothers	102:116	Healthy Mothers	102:116	The Profile of Human Milk Metabolome, Cytokines, and Antibodies in Inflammatory Bowel Diseases Versus Healthy Mothers, and Potential Impact on the Newborn.
30418545	8	15	theme	cytokines	1242:1250	arg1	levels					1215:1220	increased levels	1205:1220	increased levels of pro-inflammatory cytokines	1205:1250	In contrast, we observed that breast milk from mothers with IBD had increased levels of pro-inflammatory cytokines and higher energy metabolites [lactate and succinate] than milk from healthy mothers.
30418545	8	15	theme	cytokines	1242:1250	arg1	succinate					1295:1303	succinate	1295:1303	succinate	1295:1303	In contrast, we observed that breast milk from mothers with IBD had increased levels of pro-inflammatory cytokines and higher energy metabolites [lactate and succinate] than milk from healthy mothers.
30418545	8	15	theme	cytokines	1242:1250	arg1	metabolites					1270:1280	higher energy metabolites	1256:1280	higher energy metabolites [lactate and succinate	1256:1303	In contrast, we observed that breast milk from mothers with IBD had increased levels of pro-inflammatory cytokines and higher energy metabolites [lactate and succinate] than milk from healthy mothers.
30418545	8	15	theme	cytokines	1242:1250	arg1	[lactate					1282:1289	[lactate	1282:1289	[lactate	1282:1289	In contrast, we observed that breast milk from mothers with IBD had increased levels of pro-inflammatory cytokines and higher energy metabolites [lactate and succinate] than milk from healthy mothers.
30418545	11	16	theme	pro-inflammatory	1712:1727	arg1	cytokines					1729:1737	pro-inflammatory cytokines	1712:1737	pro-inflammatory cytokines	1712:1737	On the other hand, presence of higher levels of pro-inflammatory cytokines, lactate, and succinate may predispose the offspring to an inflammatory condition or impact on the gut microbiome.
30418545	12	17	theme	effects	1929:1935	arg1	understanding					1861:1873	Better understanding	1854:1873	Better understanding of the role of succinate in infants and its potential effects on microbiome or mucosal immunity	1854:1969	Better understanding of the role of succinate in infants and its potential effects on microbiome or mucosal immunity merits further investigations.
30418545	10	18	theme	protective	1627:1636	arg1	effects					1638:1644	the potential protective effects	1613:1644	the potential protective effects of breastfeeding	1613:1661	CONCLUSIONS The reduction in immunoprotective components of IBD breast milk such as sIgA and lactose theoretically may modulate the potential protective effects of breastfeeding.
30418545	12	19	from	infants	1903:1909	arg1	understanding					1861:1873	Better understanding	1854:1873	Better understanding of the role of succinate in infants and its potential effects on microbiome or mucosal immunity	1854:1969	Better understanding of the role of succinate in infants and its potential effects on microbiome or mucosal immunity merits further investigations.
30418545	8	20	theme	higher	1256:1261	arg1	[lactate					1282:1289	[lactate	1282:1289	[lactate	1282:1289	In contrast, we observed that breast milk from mothers with IBD had increased levels of pro-inflammatory cytokines and higher energy metabolites [lactate and succinate] than milk from healthy mothers.
30418545	8	20	theme	higher	1256:1261	arg1	metabolites					1270:1280	higher energy metabolites	1256:1280	higher energy metabolites [lactate and succinate	1256:1303	In contrast, we observed that breast milk from mothers with IBD had increased levels of pro-inflammatory cytokines and higher energy metabolites [lactate and succinate] than milk from healthy mothers.
30418545	8	20	theme	higher	1256:1261	arg1	succinate					1295:1303	succinate	1295:1303	succinate	1295:1303	In contrast, we observed that breast milk from mothers with IBD had increased levels of pro-inflammatory cytokines and higher energy metabolites [lactate and succinate] than milk from healthy mothers.
30418545	5	21	dep	Cytokines	788:796	arg1	[IgA/IgG/IgE					818:829	[IgA/IgG/IgE	818:829	[IgA/IgG/IgE	818:829	Cytokines and immunoglobulins [IgA/IgG/IgE] were analysed by multiplex Meso Scale Discovery [MSD] and commercial kits.
30418545	0	22	theme	Potential	123:131	arg1	Impact					133:138	Potential Impact	123:138	Potential Impact on the Newborn	123:153	The Profile of Human Milk Metabolome, Cytokines, and Antibodies in Inflammatory Bowel Diseases Versus Healthy Mothers, and Potential Impact on the Newborn.
30418545	5	23	theme	Scale	864:868	arg1	[MSD					880:883	multiplex Meso Scale Discovery [MSD] and commercial kits	849:904	[MSD	880:883	Cytokines and immunoglobulins [IgA/IgG/IgE] were analysed by multiplex Meso Scale Discovery [MSD] and commercial kits.
30418545	2	24	theme	potential	485:493	arg1	impact					495:500	potential impact	485:500	potential impact of IBD breast milk on neonatal immune system	485:545	We aimed to investigate whether breast milk composition differs in healthy control [HC] versus IBD mothers in terms of antibodies, cytokines, and metabolite,s to identify potential impact of IBD breast milk on neonatal immune system.
30418545	12	25	from	role	1882:1885	arg1	microbiome					1940:1949	microbiome	1940:1949	microbiome	1940:1949	Better understanding of the role of succinate in infants and its potential effects on microbiome or mucosal immunity merits further investigations.
30418545	12	25	from	role	1882:1885	arg1	immunity					1962:1969	mucosal immunity	1954:1969	mucosal immunity	1954:1969	Better understanding of the role of succinate in infants and its potential effects on microbiome or mucosal immunity merits further investigations.
30418545	12	25	from	role	1882:1885	arg1	infants					1903:1909	infants	1903:1909	infants	1903:1909	Better understanding of the role of succinate in infants and its potential effects on microbiome or mucosal immunity merits further investigations.
30418545	2	26	theme	antibodies	433:442	arg1	terms					424:428	terms	424:428	terms of antibodies, cytokines, and metabolite,s to identify potential impact of IBD breast milk on neonatal immune system	424:545	We aimed to investigate whether breast milk composition differs in healthy control [HC] versus IBD mothers in terms of antibodies, cytokines, and metabolite,s to identify potential impact of IBD breast milk on neonatal immune system.
30418545	7	27	from	mothers	1038:1044	arg1	milk					1024:1027	breast milk	1017:1027	breast milk from IBD mothers	1017:1044	RESULTS We found that breast milk from IBD mothers showed significantly lower levels of IgA, sugar metabolite [lactose], and 2-aminobutyrate.
30418545	3	28	theme	months	703:708	arg1	postpartum					710:719	3 and 6 months postpartum [PP3	695:724	3 and 6 months postpartum [PP3	695:724	METHODS Breast milk specimens from HC [n = 17] and IBD [n = 31 for Crohn's disease [CD]; and n = 41 for ulcerative colitis [UC]; were collected at 3 and 6 months postpartum [PP3] and [PP6], respectively.
30418545	12	29	theme	role	1882:1885	arg1	understanding					1861:1873	Better understanding	1854:1873	Better understanding of the role of succinate in infants and its potential effects on microbiome or mucosal immunity	1854:1969	Better understanding of the role of succinate in infants and its potential effects on microbiome or mucosal immunity merits further investigations.
30418545	9	30	theme	metabolites	1464:1474	arg1	profile					1476:1482	the milk cytokines and metabolites profile	1441:1482	the milk cytokines and metabolites profile	1441:1482	In addition, we noticed that the type of treatment [5-aminosalicylic acid versus biologics] influenced the milk cytokines and metabolites profile.
30418545	0	31	theme	Inflammatory	67:78	arg1	Diseases					86:93	Inflammatory Bowel Diseases	67:93	Inflammatory Bowel Diseases	67:93	The Profile of Human Milk Metabolome, Cytokines, and Antibodies in Inflammatory Bowel Diseases Versus Healthy Mothers, and Potential Impact on the Newborn.
30418545	1	32	theme	milk	297:300	arg1	components					302:311	their breast milk components	284:311	their breast milk components	284:311	BACKGROUND AND AIMS For women with inflammatory bowel disease [IBD], it is not very well known how IBD or IBD treatment affects their breast milk components.
30418545	12	33	from	microbiome	1940:1949	arg1	understanding					1861:1873	Better understanding	1854:1873	Better understanding of the role of succinate in infants and its potential effects on microbiome or mucosal immunity	1854:1969	Better understanding of the role of succinate in infants and its potential effects on microbiome or mucosal immunity merits further investigations.
30418545	2	34	theme	cytokines	445:453	arg1	terms					424:428	terms	424:428	terms of antibodies, cytokines, and metabolite,s to identify potential impact of IBD breast milk on neonatal immune system	424:545	We aimed to investigate whether breast milk composition differs in healthy control [HC] versus IBD mothers in terms of antibodies, cytokines, and metabolite,s to identify potential impact of IBD breast milk on neonatal immune system.
30418545	8	35	theme	healthy	1321:1327	arg1	mothers					1329:1335	healthy mothers	1321:1335	healthy mothers	1321:1335	In contrast, we observed that breast milk from mothers with IBD had increased levels of pro-inflammatory cytokines and higher energy metabolites [lactate and succinate] than milk from healthy mothers.
30418545	11	36	theme	succinate	1753:1761	arg1	levels					1702:1707	higher levels	1695:1707	higher levels of pro-inflammatory cytokines, lactate, and succinate	1695:1761	On the other hand, presence of higher levels of pro-inflammatory cytokines, lactate, and succinate may predispose the offspring to an inflammatory condition or impact on the gut microbiome.
30418545	11	37	theme	cytokines	1729:1737	arg1	levels					1702:1707	higher levels	1695:1707	higher levels of pro-inflammatory cytokines, lactate, and succinate	1695:1761	On the other hand, presence of higher levels of pro-inflammatory cytokines, lactate, and succinate may predispose the offspring to an inflammatory condition or impact on the gut microbiome.
30418545	12	38	from	effects	1929:1935	arg1	microbiome					1940:1949	microbiome	1940:1949	microbiome	1940:1949	Better understanding of the role of succinate in infants and its potential effects on microbiome or mucosal immunity merits further investigations.
30418545	12	38	from	effects	1929:1935	arg1	immunity					1962:1969	mucosal immunity	1954:1969	mucosal immunity	1954:1969	Better understanding of the role of succinate in infants and its potential effects on microbiome or mucosal immunity merits further investigations.
30418545	12	38	from	effects	1929:1935	arg1	infants					1903:1909	infants	1903:1909	infants	1903:1909	Better understanding of the role of succinate in infants and its potential effects on microbiome or mucosal immunity merits further investigations.
30418545	8	39	theme	breast	1167:1172	arg1	milk					1174:1177	breast milk	1167:1177	breast milk from mothers with IBD	1167:1199	In contrast, we observed that breast milk from mothers with IBD had increased levels of pro-inflammatory cytokines and higher energy metabolites [lactate and succinate] than milk from healthy mothers.
30418545	7	40	theme	2-aminobutyrate	1120:1134	arg1	levels					1073:1078	significantly lower levels	1053:1078	significantly lower levels of IgA, sugar metabolite [lactose], and 2-aminobutyrate	1053:1134	RESULTS We found that breast milk from IBD mothers showed significantly lower levels of IgA, sugar metabolite [lactose], and 2-aminobutyrate.
30418545	2	41	theme	healthy	381:387	arg1	[HC					397:399	healthy control [HC] versus IBD mothers	381:419	[HC	397:399	We aimed to investigate whether breast milk composition differs in healthy control [HC] versus IBD mothers in terms of antibodies, cytokines, and metabolite,s to identify potential impact of IBD breast milk on neonatal immune system.
30418545	11	42	theme	lactate	1740:1746	arg1	levels					1702:1707	higher levels	1695:1707	higher levels of pro-inflammatory cytokines, lactate, and succinate	1695:1761	On the other hand, presence of higher levels of pro-inflammatory cytokines, lactate, and succinate may predispose the offspring to an inflammatory condition or impact on the gut microbiome.
30418545	2	43	theme	milk	353:356	arg1	composition					358:368	breast milk composition	346:368	breast milk composition	346:368	We aimed to investigate whether breast milk composition differs in healthy control [HC] versus IBD mothers in terms of antibodies, cytokines, and metabolite,s to identify potential impact of IBD breast milk on neonatal immune system.
30418545	0	44	theme	Milk	21:24	arg1	Metabolome					26:35	Human Milk Metabolome	15:35	Human Milk Metabolome	15:35	The Profile of Human Milk Metabolome, Cytokines, and Antibodies in Inflammatory Bowel Diseases Versus Healthy Mothers, and Potential Impact on the Newborn.
30418545	8	45	contain	had	1201:1203	arg2	[lactate					1282:1289	[lactate	1282:1289	[lactate	1282:1289	In contrast, we observed that breast milk from mothers with IBD had increased levels of pro-inflammatory cytokines and higher energy metabolites [lactate and succinate] than milk from healthy mothers.
30418545	8	45	contain	had	1201:1203	arg2	succinate					1295:1303	succinate	1295:1303	succinate	1295:1303	In contrast, we observed that breast milk from mothers with IBD had increased levels of pro-inflammatory cytokines and higher energy metabolites [lactate and succinate] than milk from healthy mothers.
30418545	8	45	contain	had	1201:1203	arg2	levels					1215:1220	increased levels	1205:1220	increased levels of pro-inflammatory cytokines	1205:1250	In contrast, we observed that breast milk from mothers with IBD had increased levels of pro-inflammatory cytokines and higher energy metabolites [lactate and succinate] than milk from healthy mothers.
30418545	8	45	contain	had	1201:1203	arg1	milk					1174:1177	breast milk	1167:1177	breast milk from mothers with IBD	1167:1199	In contrast, we observed that breast milk from mothers with IBD had increased levels of pro-inflammatory cytokines and higher energy metabolites [lactate and succinate] than milk from healthy mothers.
30418545	8	45	contain	had	1201:1203	arg2	metabolites					1270:1280	higher energy metabolites	1256:1280	higher energy metabolites [lactate and succinate	1256:1303	In contrast, we observed that breast milk from mothers with IBD had increased levels of pro-inflammatory cytokines and higher energy metabolites [lactate and succinate] than milk from healthy mothers.
30418545	9	46	theme	[5-aminosalicylic	1389:1405	arg1	acid					1407:1410	treatment [5-aminosalicylic acid	1379:1410	treatment [5-aminosalicylic acid	1379:1410	In addition, we noticed that the type of treatment [5-aminosalicylic acid versus biologics] influenced the milk cytokines and metabolites profile.
30418545	7	47	theme	[lactose	1105:1112	arg1	levels					1073:1078	significantly lower levels	1053:1078	significantly lower levels of IgA, sugar metabolite [lactose], and 2-aminobutyrate	1053:1134	RESULTS We found that breast milk from IBD mothers showed significantly lower levels of IgA, sugar metabolite [lactose], and 2-aminobutyrate.
30418545	3	48	theme	Breast	556:561	arg1	specimens					568:576	METHODS Breast milk specimens	548:576	METHODS Breast milk specimens from HC [n	548:587	METHODS Breast milk specimens from HC [n = 17] and IBD [n = 31 for Crohn's disease [CD]; and n = 41 for ulcerative colitis [UC]; were collected at 3 and 6 months postpartum [PP3] and [PP6], respectively.
30418545	5	49	theme	Meso	859:862	arg1	[MSD					880:883	multiplex Meso Scale Discovery [MSD] and commercial kits	849:904	[MSD	880:883	Cytokines and immunoglobulins [IgA/IgG/IgE] were analysed by multiplex Meso Scale Discovery [MSD] and commercial kits.
30418545	7	50	theme	sugar	1088:1092	arg1	[lactose					1105:1112	sugar metabolite [lactose]	1088:1113	sugar metabolite [lactose]	1088:1113	RESULTS We found that breast milk from IBD mothers showed significantly lower levels of IgA, sugar metabolite [lactose], and 2-aminobutyrate.
30418545	1	51	theme	inflammatory	191:202	arg1	disease					210:216	inflammatory bowel disease	191:216	inflammatory bowel disease [IBD]	191:222	BACKGROUND AND AIMS For women with inflammatory bowel disease [IBD], it is not very well known how IBD or IBD treatment affects their breast milk components.
30418545	5	52	theme	commercial	890:899	arg1	kits					901:904	multiplex Meso Scale Discovery [MSD] and commercial kits	849:904	kits	901:904	Cytokines and immunoglobulins [IgA/IgG/IgE] were analysed by multiplex Meso Scale Discovery [MSD] and commercial kits.
30418545	7	53	theme	IgA	1083:1085	arg1	levels					1073:1078	significantly lower levels	1053:1078	significantly lower levels of IgA, sugar metabolite [lactose], and 2-aminobutyrate	1053:1134	RESULTS We found that breast milk from IBD mothers showed significantly lower levels of IgA, sugar metabolite [lactose], and 2-aminobutyrate.
30418545	8	54	theme	pro-inflammatory	1225:1240	arg1	cytokines					1242:1250	pro-inflammatory cytokines	1225:1250	pro-inflammatory cytokines	1225:1250	In contrast, we observed that breast milk from mothers with IBD had increased levels of pro-inflammatory cytokines and higher energy metabolites [lactate and succinate] than milk from healthy mothers.
30418545	10	55	theme	milk	1556:1559	arg1	sIgA					1569:1572	sIgA	1569:1572	sIgA	1569:1572	CONCLUSIONS The reduction in immunoprotective components of IBD breast milk such as sIgA and lactose theoretically may modulate the potential protective effects of breastfeeding.
30418545	10	55	theme	milk	1556:1559	arg1	components					1531:1540	immunoprotective components	1514:1540	immunoprotective components of IBD breast milk such as sIgA and lactose	1514:1584	CONCLUSIONS The reduction in immunoprotective components of IBD breast milk such as sIgA and lactose theoretically may modulate the potential protective effects of breastfeeding.
30418545	10	55	theme	milk	1556:1559	arg1	lactose					1578:1584	lactose	1578:1584	lactose	1578:1584	CONCLUSIONS The reduction in immunoprotective components of IBD breast milk such as sIgA and lactose theoretically may modulate the potential protective effects of breastfeeding.
30418545	6	56	theme	breast	917:922	arg1	metabolites					929:939	breast milk metabolites	917:939	breast milk metabolites	917:939	Moreover, breast milk metabolites were analysed by 1H nuclear magnetic resonance [NMR].
30418545	10	57	theme	IBD	1545:1547	arg1	milk					1556:1559	IBD breast milk	1545:1559	IBD breast milk	1545:1559	CONCLUSIONS The reduction in immunoprotective components of IBD breast milk such as sIgA and lactose theoretically may modulate the potential protective effects of breastfeeding.
30418545	2	58	theme	milk	516:519	arg1	impact					495:500	potential impact	485:500	potential impact of IBD breast milk on neonatal immune system	485:545	We aimed to investigate whether breast milk composition differs in healthy control [HC] versus IBD mothers in terms of antibodies, cytokines, and metabolite,s to identify potential impact of IBD breast milk on neonatal immune system.
30418545	5	59	theme	multiplex	849:857	arg1	[MSD					880:883	multiplex Meso Scale Discovery [MSD] and commercial kits	849:904	[MSD	880:883	Cytokines and immunoglobulins [IgA/IgG/IgE] were analysed by multiplex Meso Scale Discovery [MSD] and commercial kits.
30418545	12	60	from	immunity	1962:1969	arg1	understanding					1861:1873	Better understanding	1854:1873	Better understanding of the role of succinate in infants and its potential effects on microbiome or mucosal immunity	1854:1969	Better understanding of the role of succinate in infants and its potential effects on microbiome or mucosal immunity merits further investigations.
30418545	2	61	theme	IBD	505:507	arg1	milk					516:519	IBD breast milk	505:519	IBD breast milk	505:519	We aimed to investigate whether breast milk composition differs in healthy control [HC] versus IBD mothers in terms of antibodies, cytokines, and metabolite,s to identify potential impact of IBD breast milk on neonatal immune system.
30418545	2	62	from	impact	495:500	arg1	system					540:545	neonatal immune system	524:545	neonatal immune system	524:545	We aimed to investigate whether breast milk composition differs in healthy control [HC] versus IBD mothers in terms of antibodies, cytokines, and metabolite,s to identify potential impact of IBD breast milk on neonatal immune system.
30418545	8	63	from	mothers	1184:1190	arg1	milk					1174:1177	breast milk	1167:1177	breast milk from mothers with IBD	1167:1199	In contrast, we observed that breast milk from mothers with IBD had increased levels of pro-inflammatory cytokines and higher energy metabolites [lactate and succinate] than milk from healthy mothers.
30418545	2	64	theme	neonatal	524:531	arg1	system					540:545	neonatal immune system	524:545	neonatal immune system	524:545	We aimed to investigate whether breast milk composition differs in healthy control [HC] versus IBD mothers in terms of antibodies, cytokines, and metabolite,s to identify potential impact of IBD breast milk on neonatal immune system.
30418545	6	65	theme	magnetic	969:976	arg1	resonance					978:986	1H nuclear magnetic resonance	958:986	1H nuclear magnetic resonance [NMR	958:991	Moreover, breast milk metabolites were analysed by 1H nuclear magnetic resonance [NMR].
30418545	11	66	theme	gut	1838:1840	arg1	microbiome					1842:1851	the gut microbiome	1834:1851	the gut microbiome	1834:1851	On the other hand, presence of higher levels of pro-inflammatory cytokines, lactate, and succinate may predispose the offspring to an inflammatory condition or impact on the gut microbiome.
30418545	1	67	dep	disease	210:216	arg1	[IBD					218:221	[IBD	218:221	[IBD	218:221	BACKGROUND AND AIMS For women with inflammatory bowel disease [IBD], it is not very well known how IBD or IBD treatment affects their breast milk components.
30418545	6	68	theme	1H	958:959	arg1	resonance					978:986	1H nuclear magnetic resonance	958:986	1H nuclear magnetic resonance [NMR	958:991	Moreover, breast milk metabolites were analysed by 1H nuclear magnetic resonance [NMR].
30418545	8	69	with	mothers	1184:1190	arg1	IBD					1197:1199	IBD	1197:1199	IBD	1197:1199	In contrast, we observed that breast milk from mothers with IBD had increased levels of pro-inflammatory cytokines and higher energy metabolites [lactate and succinate] than milk from healthy mothers.
30418545	3	70	dep	41	645:646	arg1	[UC					671:673	[UC	671:673	[UC	671:673	METHODS Breast milk specimens from HC [n = 17] and IBD [n = 31 for Crohn's disease [CD]; and n = 41 for ulcerative colitis [UC]; were collected at 3 and 6 months postpartum [PP3] and [PP6], respectively.
30418545	3	70	dep	41	645:646	arg1	collected					682:690	collected	682:690	were collected at 3 and 6 months postpartum [PP3	677:724	METHODS Breast milk specimens from HC [n = 17] and IBD [n = 31 for Crohn's disease [CD]; and n = 41 for ulcerative colitis [UC]; were collected at 3 and 6 months postpartum [PP3] and [PP6], respectively.
30418545	2	71	theme	metabolite	460:469	arg1	terms					424:428	terms	424:428	terms of antibodies, cytokines, and metabolite,s to identify potential impact of IBD breast milk on neonatal immune system	424:545	We aimed to investigate whether breast milk composition differs in healthy control [HC] versus IBD mothers in terms of antibodies, cytokines, and metabolite,s to identify potential impact of IBD breast milk on neonatal immune system.
30418545	8	72	theme	energy	1263:1268	arg1	[lactate					1282:1289	[lactate	1282:1289	[lactate	1282:1289	In contrast, we observed that breast milk from mothers with IBD had increased levels of pro-inflammatory cytokines and higher energy metabolites [lactate and succinate] than milk from healthy mothers.
30418545	8	72	theme	energy	1263:1268	arg1	metabolites					1270:1280	higher energy metabolites	1256:1280	higher energy metabolites [lactate and succinate	1256:1303	In contrast, we observed that breast milk from mothers with IBD had increased levels of pro-inflammatory cytokines and higher energy metabolites [lactate and succinate] than milk from healthy mothers.
30418545	8	72	theme	energy	1263:1268	arg1	succinate					1295:1303	succinate	1295:1303	succinate	1295:1303	In contrast, we observed that breast milk from mothers with IBD had increased levels of pro-inflammatory cytokines and higher energy metabolites [lactate and succinate] than milk from healthy mothers.
30418545	12	73	theme	potential	1919:1927	arg1	effects					1929:1935	its potential effects	1915:1935	its potential effects on microbiome or mucosal immunity	1915:1969	Better understanding of the role of succinate in infants and its potential effects on microbiome or mucosal immunity merits further investigations.
30418545	10	74	theme	potential	1617:1625	arg1	effects					1638:1644	the potential protective effects	1613:1644	the potential protective effects of breastfeeding	1613:1661	CONCLUSIONS The reduction in immunoprotective components of IBD breast milk such as sIgA and lactose theoretically may modulate the potential protective effects of breastfeeding.
30418545	5	75	theme	Discovery	870:878	arg1	[MSD					880:883	multiplex Meso Scale Discovery [MSD] and commercial kits	849:904	[MSD	880:883	Cytokines and immunoglobulins [IgA/IgG/IgE] were analysed by multiplex Meso Scale Discovery [MSD] and commercial kits.
30418545	11	76	theme	inflammatory	1798:1809	arg1	condition					1811:1819	an inflammatory condition	1795:1819	an inflammatory condition	1795:1819	On the other hand, presence of higher levels of pro-inflammatory cytokines, lactate, and succinate may predispose the offspring to an inflammatory condition or impact on the gut microbiome.
30418545	3	77	theme	ulcerative	652:661	arg1	colitis					663:669	ulcerative colitis	652:669	ulcerative colitis	652:669	METHODS Breast milk specimens from HC [n = 17] and IBD [n = 31 for Crohn's disease [CD]; and n = 41 for ulcerative colitis [UC]; were collected at 3 and 6 months postpartum [PP3] and [PP6], respectively.
30418545	7	78	theme	IBD	1034:1036	arg1	mothers					1038:1044	IBD mothers	1034:1044	IBD mothers	1034:1044	RESULTS We found that breast milk from IBD mothers showed significantly lower levels of IgA, sugar metabolite [lactose], and 2-aminobutyrate.
30418545	10	79	theme	breastfeeding	1649:1661	arg1	effects					1638:1644	the potential protective effects	1613:1644	the potential protective effects of breastfeeding	1613:1661	CONCLUSIONS The reduction in immunoprotective components of IBD breast milk such as sIgA and lactose theoretically may modulate the potential protective effects of breastfeeding.
30418545	7	80	theme	breast	1017:1022	arg1	milk					1024:1027	breast milk	1017:1027	breast milk from IBD mothers	1017:1044	RESULTS We found that breast milk from IBD mothers showed significantly lower levels of IgA, sugar metabolite [lactose], and 2-aminobutyrate.
30418545	12	81	from	understanding	1861:1873	arg1	microbiome					1940:1949	microbiome	1940:1949	microbiome	1940:1949	Better understanding of the role of succinate in infants and its potential effects on microbiome or mucosal immunity merits further investigations.
30418545	12	81	from	understanding	1861:1873	arg1	immunity					1962:1969	mucosal immunity	1954:1969	mucosal immunity	1954:1969	Better understanding of the role of succinate in infants and its potential effects on microbiome or mucosal immunity merits further investigations.
30418545	12	81	from	understanding	1861:1873	arg1	infants					1903:1909	infants	1903:1909	infants	1903:1909	Better understanding of the role of succinate in infants and its potential effects on microbiome or mucosal immunity merits further investigations.
30418545	0	82	from	Profile	4:10	arg1	Newborn					147:153	Newborn	147:153	Newborn	147:153	The Profile of Human Milk Metabolome, Cytokines, and Antibodies in Inflammatory Bowel Diseases Versus Healthy Mothers, and Potential Impact on the Newborn.
30418545	0	82	from	Profile	4:10	arg1	Diseases					86:93	Inflammatory Bowel Diseases	67:93	Inflammatory Bowel Diseases	67:93	The Profile of Human Milk Metabolome, Cytokines, and Antibodies in Inflammatory Bowel Diseases Versus Healthy Mothers, and Potential Impact on the Newborn.
30418545	0	82	from	Profile	4:10	arg1	Mothers					110:116	Healthy Mothers	102:116	Healthy Mothers	102:116	The Profile of Human Milk Metabolome, Cytokines, and Antibodies in Inflammatory Bowel Diseases Versus Healthy Mothers, and Potential Impact on the Newborn.
30418545	0	83	theme	Bowel	80:84	arg1	Diseases					86:93	Inflammatory Bowel Diseases	67:93	Inflammatory Bowel Diseases	67:93	The Profile of Human Milk Metabolome, Cytokines, and Antibodies in Inflammatory Bowel Diseases Versus Healthy Mothers, and Potential Impact on the Newborn.
30418545	1	84	theme	breast	290:295	arg1	components					302:311	their breast milk components	284:311	their breast milk components	284:311	BACKGROUND AND AIMS For women with inflammatory bowel disease [IBD], it is not very well known how IBD or IBD treatment affects their breast milk components.
30418545	4	85	theme	Faecal	752:757	arg1	samples					759:765	Faecal samples	752:765	Faecal samples	752:765	Faecal samples were also collected.
30418545	0	86	from	Cytokines	38:46	arg1	Newborn					147:153	Newborn	147:153	Newborn	147:153	The Profile of Human Milk Metabolome, Cytokines, and Antibodies in Inflammatory Bowel Diseases Versus Healthy Mothers, and Potential Impact on the Newborn.
30418545	0	86	from	Cytokines	38:46	arg1	Diseases					86:93	Inflammatory Bowel Diseases	67:93	Inflammatory Bowel Diseases	67:93	The Profile of Human Milk Metabolome, Cytokines, and Antibodies in Inflammatory Bowel Diseases Versus Healthy Mothers, and Potential Impact on the Newborn.
30418545	0	86	from	Cytokines	38:46	arg1	Mothers					110:116	Healthy Mothers	102:116	Healthy Mothers	102:116	The Profile of Human Milk Metabolome, Cytokines, and Antibodies in Inflammatory Bowel Diseases Versus Healthy Mothers, and Potential Impact on the Newborn.
30418545	7	87	dep	RESULTS	995:1001	arg1	found					1006:1010	found	1006:1010	found that breast milk from IBD mothers showed significantly lower levels of IgA, sugar metabolite [lactose], and 2-aminobutyrate	1006:1134	RESULTS We found that breast milk from IBD mothers showed significantly lower levels of IgA, sugar metabolite [lactose], and 2-aminobutyrate.
30418545	11	88	theme	other	1671:1675	arg1	hand					1677:1680	the other hand	1667:1680	the other hand	1667:1680	On the other hand, presence of higher levels of pro-inflammatory cytokines, lactate, and succinate may predispose the offspring to an inflammatory condition or impact on the gut microbiome.
30418545	2	89	theme	control	389:395	arg1	[HC					397:399	healthy control [HC] versus IBD mothers	381:419	[HC	397:399	We aimed to investigate whether breast milk composition differs in healthy control [HC] versus IBD mothers in terms of antibodies, cytokines, and metabolite,s to identify potential impact of IBD breast milk on neonatal immune system.
30418545	0	90	from	Antibodies	53:62	arg1	Newborn					147:153	Newborn	147:153	Newborn	147:153	The Profile of Human Milk Metabolome, Cytokines, and Antibodies in Inflammatory Bowel Diseases Versus Healthy Mothers, and Potential Impact on the Newborn.
30418545	0	90	from	Antibodies	53:62	arg1	Diseases					86:93	Inflammatory Bowel Diseases	67:93	Inflammatory Bowel Diseases	67:93	The Profile of Human Milk Metabolome, Cytokines, and Antibodies in Inflammatory Bowel Diseases Versus Healthy Mothers, and Potential Impact on the Newborn.
30418545	0	90	from	Antibodies	53:62	arg1	Mothers					110:116	Healthy Mothers	102:116	Healthy Mothers	102:116	The Profile of Human Milk Metabolome, Cytokines, and Antibodies in Inflammatory Bowel Diseases Versus Healthy Mothers, and Potential Impact on the Newborn.
30418545	0	91	theme	Human	15:19	arg1	Metabolome					26:35	Human Milk Metabolome	15:35	Human Milk Metabolome	15:35	The Profile of Human Milk Metabolome, Cytokines, and Antibodies in Inflammatory Bowel Diseases Versus Healthy Mothers, and Potential Impact on the Newborn.
30418545	1	92	with	women	180:184	arg1	disease					210:216	inflammatory bowel disease	191:216	inflammatory bowel disease [IBD]	191:222	BACKGROUND AND AIMS For women with inflammatory bowel disease [IBD], it is not very well known how IBD or IBD treatment affects their breast milk components.
30418545	3	93	dep	postpartum	710:719	arg1	[PP3					721:724	[PP3	721:724	3 and 6 months postpartum [PP3	695:724	METHODS Breast milk specimens from HC [n = 17] and IBD [n = 31 for Crohn's disease [CD]; and n = 41 for ulcerative colitis [UC]; were collected at 3 and 6 months postpartum [PP3] and [PP6], respectively.
30418545	3	94	theme	milk	563:566	arg1	specimens					568:576	METHODS Breast milk specimens	548:576	METHODS Breast milk specimens from HC [n	548:587	METHODS Breast milk specimens from HC [n = 17] and IBD [n = 31 for Crohn's disease [CD]; and n = 41 for ulcerative colitis [UC]; were collected at 3 and 6 months postpartum [PP3] and [PP6], respectively.
30418545	6	95	theme	milk	924:927	arg1	metabolites					929:939	breast milk metabolites	917:939	breast milk metabolites	917:939	Moreover, breast milk metabolites were analysed by 1H nuclear magnetic resonance [NMR].
30418545	0	96	theme	Metabolome	26:35	arg1	Antibodies					53:62	Antibodies	53:62	Antibodies in Inflammatory Bowel Diseases Versus Healthy Mothers	53:116	The Profile of Human Milk Metabolome, Cytokines, and Antibodies in Inflammatory Bowel Diseases Versus Healthy Mothers, and Potential Impact on the Newborn.
30418545	0	96	theme	Metabolome	26:35	arg1	Impact					133:138	Potential Impact	123:138	Potential Impact on the Newborn	123:153	The Profile of Human Milk Metabolome, Cytokines, and Antibodies in Inflammatory Bowel Diseases Versus Healthy Mothers, and Potential Impact on the Newborn.
30418545	0	96	theme	Metabolome	26:35	arg1	Profile					4:10	The Profile	0:10	The Profile of Human Milk Metabolome	0:35	The Profile of Human Milk Metabolome, Cytokines, and Antibodies in Inflammatory Bowel Diseases Versus Healthy Mothers, and Potential Impact on the Newborn.
30418545	0	96	theme	Metabolome	26:35	arg1	Cytokines					38:46	Cytokines	38:46	Cytokines	38:46	The Profile of Human Milk Metabolome, Cytokines, and Antibodies in Inflammatory Bowel Diseases Versus Healthy Mothers, and Potential Impact on the Newborn.
30418545	8	97	dep	metabolites	1270:1280	arg1	[lactate					1282:1289	[lactate	1282:1289	[lactate	1282:1289	In contrast, we observed that breast milk from mothers with IBD had increased levels of pro-inflammatory cytokines and higher energy metabolites [lactate and succinate] than milk from healthy mothers.
30418545	8	97	dep	metabolites	1270:1280	arg1	metabolites					1270:1280	higher energy metabolites	1256:1280	higher energy metabolites [lactate and succinate	1256:1303	In contrast, we observed that breast milk from mothers with IBD had increased levels of pro-inflammatory cytokines and higher energy metabolites [lactate and succinate] than milk from healthy mothers.
30418545	8	97	dep	metabolites	1270:1280	arg1	succinate					1295:1303	succinate	1295:1303	succinate	1295:1303	In contrast, we observed that breast milk from mothers with IBD had increased levels of pro-inflammatory cytokines and higher energy metabolites [lactate and succinate] than milk from healthy mothers.
30418545	9	98	theme	treatment	1379:1387	arg1	acid					1407:1410	treatment [5-aminosalicylic acid	1379:1410	treatment [5-aminosalicylic acid	1379:1410	In addition, we noticed that the type of treatment [5-aminosalicylic acid versus biologics] influenced the milk cytokines and metabolites profile.
30418545	7	99	theme	metabolite	1094:1103	arg1	[lactose					1105:1112	sugar metabolite [lactose]	1088:1113	sugar metabolite [lactose]	1088:1113	RESULTS We found that breast milk from IBD mothers showed significantly lower levels of IgA, sugar metabolite [lactose], and 2-aminobutyrate.
30418545	2	100	theme	IBD	409:411	arg1	mothers					413:419	healthy control [HC] versus IBD mothers	381:419	mothers	413:419	We aimed to investigate whether breast milk composition differs in healthy control [HC] versus IBD mothers in terms of antibodies, cytokines, and metabolite,s to identify potential impact of IBD breast milk on neonatal immune system.
30418545	3	101	theme	METHODS	548:554	arg1	specimens					568:576	METHODS Breast milk specimens	548:576	METHODS Breast milk specimens from HC [n	548:587	METHODS Breast milk specimens from HC [n = 17] and IBD [n = 31 for Crohn's disease [CD]; and n = 41 for ulcerative colitis [UC]; were collected at 3 and 6 months postpartum [PP3] and [PP6], respectively.
30418545	2	102	theme	breast	346:351	arg1	composition					358:368	breast milk composition	346:368	breast milk composition	346:368	We aimed to investigate whether breast milk composition differs in healthy control [HC] versus IBD mothers in terms of antibodies, cytokines, and metabolite,s to identify potential impact of IBD breast milk on neonatal immune system.
30418545	8	103	from	mothers	1329:1335	arg1	milk					1311:1314	milk	1311:1314	milk from healthy mothers	1311:1335	In contrast, we observed that breast milk from mothers with IBD had increased levels of pro-inflammatory cytokines and higher energy metabolites [lactate and succinate] than milk from healthy mothers.
30418545	1	104	theme	IBD	255:257	arg1	treatment					266:274	IBD or IBD treatment	255:274	IBD or IBD treatment	255:274	BACKGROUND AND AIMS For women with inflammatory bowel disease [IBD], it is not very well known how IBD or IBD treatment affects their breast milk components.
30418545	11	105	theme	higher	1695:1700	arg1	levels					1702:1707	higher levels	1695:1707	higher levels of pro-inflammatory cytokines, lactate, and succinate	1695:1761	On the other hand, presence of higher levels of pro-inflammatory cytokines, lactate, and succinate may predispose the offspring to an inflammatory condition or impact on the gut microbiome.
30418545	9	106	theme	acid	1407:1410	arg1	type					1371:1374	the type	1367:1374	the type of treatment [5-aminosalicylic acid versus biologics]	1367:1428	In addition, we noticed that the type of treatment [5-aminosalicylic acid versus biologics] influenced the milk cytokines and metabolites profile.
30418545	10	107	dep	CONCLUSIONS	1485:1495	arg1	modulate					1604:1611	modulate	1604:1611	may modulate the potential protective effects of breastfeeding	1600:1661	CONCLUSIONS The reduction in immunoprotective components of IBD breast milk such as sIgA and lactose theoretically may modulate the potential protective effects of breastfeeding.
30418545	10	108	theme	immunoprotective	1514:1529	arg1	sIgA					1569:1572	sIgA	1569:1572	sIgA	1569:1572	CONCLUSIONS The reduction in immunoprotective components of IBD breast milk such as sIgA and lactose theoretically may modulate the potential protective effects of breastfeeding.
30418545	10	108	theme	immunoprotective	1514:1529	arg1	components					1531:1540	immunoprotective components	1514:1540	immunoprotective components of IBD breast milk such as sIgA and lactose	1514:1584	CONCLUSIONS The reduction in immunoprotective components of IBD breast milk such as sIgA and lactose theoretically may modulate the potential protective effects of breastfeeding.
30418545	10	108	theme	immunoprotective	1514:1529	arg1	lactose					1578:1584	lactose	1578:1584	lactose	1578:1584	CONCLUSIONS The reduction in immunoprotective components of IBD breast milk such as sIgA and lactose theoretically may modulate the potential protective effects of breastfeeding.
30418545	1	109	theme	IBD	262:264	arg1	treatment					266:274	IBD or IBD treatment	255:274	IBD or IBD treatment	255:274	BACKGROUND AND AIMS For women with inflammatory bowel disease [IBD], it is not very well known how IBD or IBD treatment affects their breast milk components.
30418545	9	110	theme	biologics	1419:1427	arg1	type					1371:1374	the type	1367:1374	the type of treatment [5-aminosalicylic acid versus biologics]	1367:1428	In addition, we noticed that the type of treatment [5-aminosalicylic acid versus biologics] influenced the milk cytokines and metabolites profile.
30418545	8	111	theme	increased	1205:1213	arg1	levels					1215:1220	increased levels	1205:1220	increased levels of pro-inflammatory cytokines	1205:1250	In contrast, we observed that breast milk from mothers with IBD had increased levels of pro-inflammatory cytokines and higher energy metabolites [lactate and succinate] than milk from healthy mothers.
30418545	11	112	theme	levels	1702:1707	arg1	presence					1683:1690	presence	1683:1690	presence of higher levels of pro-inflammatory cytokines, lactate, and succinate	1683:1761	On the other hand, presence of higher levels of pro-inflammatory cytokines, lactate, and succinate may predispose the offspring to an inflammatory condition or impact on the gut microbiome.
30418545	1	113	theme	bowel	204:208	arg1	disease					210:216	inflammatory bowel disease	191:216	inflammatory bowel disease [IBD]	191:222	BACKGROUND AND AIMS For women with inflammatory bowel disease [IBD], it is not very well known how IBD or IBD treatment affects their breast milk components.
30418545	7	114	theme	lower	1067:1071	arg1	levels					1073:1078	significantly lower levels	1053:1078	significantly lower levels of IgA, sugar metabolite [lactose], and 2-aminobutyrate	1053:1134	RESULTS We found that breast milk from IBD mothers showed significantly lower levels of IgA, sugar metabolite [lactose], and 2-aminobutyrate.
30418545	3	115	from	HC	583:584	arg1	specimens					568:576	METHODS Breast milk specimens	548:576	METHODS Breast milk specimens from HC [n	548:587	METHODS Breast milk specimens from HC [n = 17] and IBD [n = 31 for Crohn's disease [CD]; and n = 41 for ulcerative colitis [UC]; were collected at 3 and 6 months postpartum [PP3] and [PP6], respectively.
30418545	12	116	theme	further	1978:1984	arg1	investigations					1986:1999	further investigations	1978:1999	further investigations	1978:1999	Better understanding of the role of succinate in infants and its potential effects on microbiome or mucosal immunity merits further investigations.
30418545	10	117	theme	breast	1549:1554	arg1	milk					1556:1559	IBD breast milk	1545:1559	IBD breast milk	1545:1559	CONCLUSIONS The reduction in immunoprotective components of IBD breast milk such as sIgA and lactose theoretically may modulate the potential protective effects of breastfeeding.
29540768	2	0	theme	pigs	406:409	arg1	cecum					349:353	cecum	349:353	cecum	349:353	In this study, we performed a comparative analysis of the gut microbiome in the ileum, cecum, and colon of Duroc × (Landrace × Yorkshire) (DLY) pigs showing two extreme feed conversion ratios (FCRs) using 16S rRNA gene sequencing.
29540768	2	0	theme	pigs	406:409	arg1	colon					360:364	colon	360:364	colon	360:364	In this study, we performed a comparative analysis of the gut microbiome in the ileum, cecum, and colon of Duroc × (Landrace × Yorkshire) (DLY) pigs showing two extreme feed conversion ratios (FCRs) using 16S rRNA gene sequencing.
29540768	2	0	theme	pigs	406:409	arg1	ileum					342:346	the ileum	338:346	the ileum	338:346	In this study, we performed a comparative analysis of the gut microbiome in the ileum, cecum, and colon of Duroc × (Landrace × Yorkshire) (DLY) pigs showing two extreme feed conversion ratios (FCRs) using 16S rRNA gene sequencing.
29540768	0	1	from	comparison	9:18	arg1	pigs					88:91	commercial pigs	77:91	commercial pigs	77:91	A global comparison of the microbiome compositions of three gut locations in commercial pigs with extreme feed conversion ratios.
29540768	6	2	theme	metabolic	979:987	arg1	pathways					989:996	two and nine metabolic pathways	966:996	pathways	989:996	We then identified two and nine metabolic pathways that were enriched in the cecum and colon of the high FCR pigs, respectively.
29540768	0	3	theme	locations	64:72	arg1	compositions					38:49	the microbiome compositions	23:49	the microbiome compositions of three gut locations in commercial pigs	23:91	A global comparison of the microbiome compositions of three gut locations in commercial pigs with extreme feed conversion ratios.
29540768	3	4	theme	alpha	591:595	arg1	diversity					597:605	significantly higher alpha diversity	570:605	significantly higher alpha diversity	570:605	The results revealed that the microbial community in the cecum and colon had significantly higher alpha diversity than the ileum.
29540768	4	5	theme	high	756:759	arg1	pigs					773:776	the high and low FCR pigs	752:776	the high and low FCR pigs	752:776	We further identified 11, 55, and 55 operational taxonomic units (OTUs) with significantly different relative abundances between the high and low FCR pigs among the three gut locations, respectively.
29540768	6	6	theme	nine	974:977	arg1	pathways					989:996	two and nine metabolic pathways	966:996	pathways	989:996	We then identified two and nine metabolic pathways that were enriched in the cecum and colon of the high FCR pigs, respectively.
29540768	4	7	theme	gut	794:796	arg1	locations					798:806	the three gut locations	784:806	the three gut locations	784:806	We further identified 11, 55, and 55 operational taxonomic units (OTUs) with significantly different relative abundances between the high and low FCR pigs among the three gut locations, respectively.
29540768	8	8	theme	gut	1403:1405	arg1	microbiota					1407:1416	gut microbiota	1403:1416	gut microbiota	1403:1416	These results should improve our understanding of microbiota compositions in the different gut locations of commercial pigs and provide important insights into the effect of gut microbiota on porcine FCRs.
29540768	4	9	theme	FCR	769:771	arg1	pigs					773:776	the high and low FCR pigs	752:776	the high and low FCR pigs	752:776	We further identified 11, 55, and 55 operational taxonomic units (OTUs) with significantly different relative abundances between the high and low FCR pigs among the three gut locations, respectively.
29540768	5	10	theme	dietary	909:915	arg1	polysaccharides					917:931	dietary polysaccharides	909:931	dietary polysaccharides	909:931	These OTUs were mainly associated with bacteria that participate in the metabolism of dietary polysaccharides and proteins.
29540768	6	11	theme	pigs	1056:1059	arg1	colon					1034:1038	colon	1034:1038	colon	1034:1038	We then identified two and nine metabolic pathways that were enriched in the cecum and colon of the high FCR pigs, respectively.
29540768	6	11	theme	pigs	1056:1059	arg1	cecum					1024:1028	cecum	1024:1028	cecum	1024:1028	We then identified two and nine metabolic pathways that were enriched in the cecum and colon of the high FCR pigs, respectively.
29540768	7	12	dep	acids	1125:1129	arg1	the					1103:1105	the	1103:1105	the	1103:1105	The results suggested that the short chain fatty acids and indolic compounds produced by microbial fermentation might influence porcine feed efficiency.
29540768	4	13	with	units	682:686	arg1	abundances					733:742	significantly different relative abundances	700:742	significantly different relative abundances between the high and low FCR pigs	700:776	We further identified 11, 55, and 55 operational taxonomic units (OTUs) with significantly different relative abundances between the high and low FCR pigs among the three gut locations, respectively.
29540768	0	14	theme	commercial	77:86	arg1	pigs					88:91	commercial pigs	77:91	commercial pigs	77:91	A global comparison of the microbiome compositions of three gut locations in commercial pigs with extreme feed conversion ratios.
29540768	8	15	theme	porcine	1421:1427	arg1	FCRs					1429:1432	porcine FCRs	1421:1432	porcine FCRs	1421:1432	These results should improve our understanding of microbiota compositions in the different gut locations of commercial pigs and provide important insights into the effect of gut microbiota on porcine FCRs.
29540768	4	16	theme	low	765:767	arg1	pigs					773:776	the high and low FCR pigs	752:776	the high and low FCR pigs	752:776	We further identified 11, 55, and 55 operational taxonomic units (OTUs) with significantly different relative abundances between the high and low FCR pigs among the three gut locations, respectively.
29540768	6	17	theme	FCR	1052:1054	arg1	pigs					1056:1059	the high FCR pigs	1043:1059	the high FCR pigs	1043:1059	We then identified two and nine metabolic pathways that were enriched in the cecum and colon of the high FCR pigs, respectively.
29540768	6	18	dep	cecum	1024:1028	arg1	the					1020:1022	the	1020:1022	the	1020:1022	We then identified two and nine metabolic pathways that were enriched in the cecum and colon of the high FCR pigs, respectively.
29540768	0	19	theme	extreme	98:104	arg1	ratios					122:127	extreme feed conversion ratios	98:127	extreme feed conversion ratios	98:127	A global comparison of the microbiome compositions of three gut locations in commercial pigs with extreme feed conversion ratios.
29540768	0	20	from	compositions	38:49	arg1	pigs					88:91	commercial pigs	77:91	commercial pigs	77:91	A global comparison of the microbiome compositions of three gut locations in commercial pigs with extreme feed conversion ratios.
29540768	4	21	theme	operational	660:670	arg1	OTUs					689:692	OTUs	689:692	OTUs	689:692	We further identified 11, 55, and 55 operational taxonomic units (OTUs) with significantly different relative abundances between the high and low FCR pigs among the three gut locations, respectively.
29540768	4	21	theme	operational	660:670	arg1	units					682:686	11, 55, and 55 operational taxonomic units	645:686	11, 55, and 55 operational taxonomic units (OTUs) with significantly different relative abundances between the high and low FCR pigs	645:776	We further identified 11, 55, and 55 operational taxonomic units (OTUs) with significantly different relative abundances between the high and low FCR pigs among the three gut locations, respectively.
29540768	3	22	contain	had	566:568	arg2	diversity					597:605	significantly higher alpha diversity	570:605	significantly higher alpha diversity	570:605	The results revealed that the microbial community in the cecum and colon had significantly higher alpha diversity than the ileum.
29540768	3	22	contain	had	566:568	arg1	community					533:541	the microbial community	519:541	the microbial community in the cecum and colon	519:564	The results revealed that the microbial community in the cecum and colon had significantly higher alpha diversity than the ileum.
29540768	2	23	theme	extreme	423:429	arg1	FCRs					455:458	FCRs	455:458	FCRs	455:458	In this study, we performed a comparative analysis of the gut microbiome in the ileum, cecum, and colon of Duroc × (Landrace × Yorkshire) (DLY) pigs showing two extreme feed conversion ratios (FCRs) using 16S rRNA gene sequencing.
29540768	2	23	theme	extreme	423:429	arg1	ratios					447:452	two extreme feed conversion ratios	419:452	two extreme feed conversion ratios (FCRs)	419:459	In this study, we performed a comparative analysis of the gut microbiome in the ileum, cecum, and colon of Duroc × (Landrace × Yorkshire) (DLY) pigs showing two extreme feed conversion ratios (FCRs) using 16S rRNA gene sequencing.
29540768	4	24	theme	relative	724:731	arg1	abundances					733:742	significantly different relative abundances	700:742	significantly different relative abundances between the high and low FCR pigs	700:776	We further identified 11, 55, and 55 operational taxonomic units (OTUs) with significantly different relative abundances between the high and low FCR pigs among the three gut locations, respectively.
29540768	8	25	theme	important	1365:1373	arg1	insights					1375:1382	important insights	1365:1382	important insights into the effect of gut microbiota on porcine FCRs	1365:1432	These results should improve our understanding of microbiota compositions in the different gut locations of commercial pigs and provide important insights into the effect of gut microbiota on porcine FCRs.
29540768	2	26	theme	feed	431:434	arg1	FCRs					455:458	FCRs	455:458	FCRs	455:458	In this study, we performed a comparative analysis of the gut microbiome in the ileum, cecum, and colon of Duroc × (Landrace × Yorkshire) (DLY) pigs showing two extreme feed conversion ratios (FCRs) using 16S rRNA gene sequencing.
29540768	2	26	theme	feed	431:434	arg1	ratios					447:452	two extreme feed conversion ratios	419:452	two extreme feed conversion ratios (FCRs)	419:459	In this study, we performed a comparative analysis of the gut microbiome in the ileum, cecum, and colon of Duroc × (Landrace × Yorkshire) (DLY) pigs showing two extreme feed conversion ratios (FCRs) using 16S rRNA gene sequencing.
29540768	0	27	theme	global	2:7	arg1	comparison					9:18	A global comparison	0:18	A global comparison of the microbiome compositions of three gut locations in commercial pigs with extreme feed conversion ratios.	0:128	A global comparison of the microbiome compositions of three gut locations in commercial pigs with extreme feed conversion ratios.
29540768	8	28	theme	different	1310:1318	arg1	locations					1324:1332	the different gut locations	1306:1332	the different gut locations of commercial pigs	1306:1351	These results should improve our understanding of microbiota compositions in the different gut locations of commercial pigs and provide important insights into the effect of gut microbiota on porcine FCRs.
29540768	3	29	from	community	533:541	arg1	cecum					550:554	cecum	550:554	cecum	550:554	The results revealed that the microbial community in the cecum and colon had significantly higher alpha diversity than the ileum.
29540768	3	29	from	community	533:541	arg1	colon					560:564	colon	560:564	colon	560:564	The results revealed that the microbial community in the cecum and colon had significantly higher alpha diversity than the ileum.
29540768	2	30	theme	Duroc × 	369:376	arg1	pigs					406:409	Duroc × (Landrace × Yorkshire) (DLY) pigs	369:409	Duroc × (Landrace × Yorkshire) (DLY) pigs showing two extreme feed conversion ratios (FCRs)	369:459	In this study, we performed a comparative analysis of the gut microbiome in the ileum, cecum, and colon of Duroc × (Landrace × Yorkshire) (DLY) pigs showing two extreme feed conversion ratios (FCRs) using 16S rRNA gene sequencing.
29540768	2	31	theme	microbiome	324:333	arg1	analysis					304:311	a comparative analysis	290:311	a comparative analysis of the gut microbiome	290:333	In this study, we performed a comparative analysis of the gut microbiome in the ileum, cecum, and colon of Duroc × (Landrace × Yorkshire) (DLY) pigs showing two extreme feed conversion ratios (FCRs) using 16S rRNA gene sequencing.
29540768	1	32	theme	much	247:250	arg1	research					252:259	much research	247:259	much research	247:259	In an attempt to increase profits and sustainability in the swine industry, the gut microbiome has become a focus of much research.
29540768	0	33	theme	conversion	111:120	arg1	ratios					122:127	extreme feed conversion ratios	98:127	extreme feed conversion ratios	98:127	A global comparison of the microbiome compositions of three gut locations in commercial pigs with extreme feed conversion ratios.
29540768	5	34	theme	polysaccharides	917:931	arg1	metabolism					895:904	the metabolism	891:904	the metabolism of dietary polysaccharides and proteins	891:944	These OTUs were mainly associated with bacteria that participate in the metabolism of dietary polysaccharides and proteins.
29540768	8	35	from	effect	1393:1398	arg1	FCRs					1429:1432	porcine FCRs	1421:1432	porcine FCRs	1421:1432	These results should improve our understanding of microbiota compositions in the different gut locations of commercial pigs and provide important insights into the effect of gut microbiota on porcine FCRs.
29540768	1	36	theme	research	252:259	arg1	focus					238:242	a focus	236:242	a focus of much research	236:259	In an attempt to increase profits and sustainability in the swine industry, the gut microbiome has become a focus of much research.
29540768	0	37	theme	feed	106:109	arg1	ratios					122:127	extreme feed conversion ratios	98:127	extreme feed conversion ratios	98:127	A global comparison of the microbiome compositions of three gut locations in commercial pigs with extreme feed conversion ratios.
29540768	8	38	theme	pigs	1348:1351	arg1	locations					1324:1332	the different gut locations	1306:1332	the different gut locations of commercial pigs	1306:1351	These results should improve our understanding of microbiota compositions in the different gut locations of commercial pigs and provide important insights into the effect of gut microbiota on porcine FCRs.
29540768	7	39	theme	chain	1113:1117	arg1	acids					1125:1129	short chain fatty acids	1107:1129	short chain fatty acids	1107:1129	The results suggested that the short chain fatty acids and indolic compounds produced by microbial fermentation might influence porcine feed efficiency.
29540768	7	40	theme	fatty	1119:1123	arg1	acids					1125:1129	short chain fatty acids	1107:1129	short chain fatty acids	1107:1129	The results suggested that the short chain fatty acids and indolic compounds produced by microbial fermentation might influence porcine feed efficiency.
29540768	0	41	theme	microbiome	27:36	arg1	compositions					38:49	the microbiome compositions	23:49	the microbiome compositions of three gut locations in commercial pigs	23:91	A global comparison of the microbiome compositions of three gut locations in commercial pigs with extreme feed conversion ratios.
29540768	8	42	theme	commercial	1337:1346	arg1	pigs					1348:1351	commercial pigs	1337:1351	commercial pigs	1337:1351	These results should improve our understanding of microbiota compositions in the different gut locations of commercial pigs and provide important insights into the effect of gut microbiota on porcine FCRs.
29540768	8	43	theme	compositions	1290:1301	arg1	understanding					1262:1274	our understanding	1258:1274	our understanding of microbiota compositions	1258:1301	These results should improve our understanding of microbiota compositions in the different gut locations of commercial pigs and provide important insights into the effect of gut microbiota on porcine FCRs.
29540768	7	44	theme	porcine	1204:1210	arg1	efficiency					1217:1226	porcine feed efficiency	1204:1226	porcine feed efficiency	1204:1226	The results suggested that the short chain fatty acids and indolic compounds produced by microbial fermentation might influence porcine feed efficiency.
29540768	1	45	theme	swine	190:194	arg1	industry					196:203	the swine industry	186:203	the swine industry	186:203	In an attempt to increase profits and sustainability in the swine industry, the gut microbiome has become a focus of much research.
29540768	7	46	theme	feed	1212:1215	arg1	efficiency					1217:1226	porcine feed efficiency	1204:1226	porcine feed efficiency	1204:1226	The results suggested that the short chain fatty acids and indolic compounds produced by microbial fermentation might influence porcine feed efficiency.
29540768	8	47	theme	microbiota	1279:1288	arg1	compositions					1290:1301	microbiota compositions	1279:1301	microbiota compositions	1279:1301	These results should improve our understanding of microbiota compositions in the different gut locations of commercial pigs and provide important insights into the effect of gut microbiota on porcine FCRs.
29540768	7	48	theme	microbial	1165:1173	arg1	fermentation					1175:1186	microbial fermentation	1165:1186	microbial fermentation	1165:1186	The results suggested that the short chain fatty acids and indolic compounds produced by microbial fermentation might influence porcine feed efficiency.
29540768	4	49	theme	different	714:722	arg1	abundances					733:742	significantly different relative abundances	700:742	significantly different relative abundances between the high and low FCR pigs	700:776	We further identified 11, 55, and 55 operational taxonomic units (OTUs) with significantly different relative abundances between the high and low FCR pigs among the three gut locations, respectively.
29540768	0	50	with	comparison	9:18	arg1	ratios					122:127	extreme feed conversion ratios	98:127	extreme feed conversion ratios	98:127	A global comparison of the microbiome compositions of three gut locations in commercial pigs with extreme feed conversion ratios.
29540768	2	51	theme	comparative	292:302	arg1	analysis					304:311	a comparative analysis	290:311	a comparative analysis of the gut microbiome	290:333	In this study, we performed a comparative analysis of the gut microbiome in the ileum, cecum, and colon of Duroc × (Landrace × Yorkshire) (DLY) pigs showing two extreme feed conversion ratios (FCRs) using 16S rRNA gene sequencing.
29540768	0	52	theme	compositions	38:49	arg1	comparison					9:18	A global comparison	0:18	A global comparison of the microbiome compositions of three gut locations in commercial pigs with extreme feed conversion ratios.	0:128	A global comparison of the microbiome compositions of three gut locations in commercial pigs with extreme feed conversion ratios.
29540768	2	53	theme	gene	476:479	arg1	sequencing					481:490	16S rRNA gene sequencing	467:490	16S rRNA gene sequencing	467:490	In this study, we performed a comparative analysis of the gut microbiome in the ileum, cecum, and colon of Duroc × (Landrace × Yorkshire) (DLY) pigs showing two extreme feed conversion ratios (FCRs) using 16S rRNA gene sequencing.
29540768	5	54	theme	proteins	937:944	arg1	metabolism					895:904	the metabolism	891:904	the metabolism of dietary polysaccharides and proteins	891:944	These OTUs were mainly associated with bacteria that participate in the metabolism of dietary polysaccharides and proteins.
29540768	8	55	theme	microbiota	1407:1416	arg1	effect					1393:1398	the effect	1389:1398	the effect of gut microbiota on porcine FCRs	1389:1432	These results should improve our understanding of microbiota compositions in the different gut locations of commercial pigs and provide important insights into the effect of gut microbiota on porcine FCRs.
29540768	8	56	theme	gut	1320:1322	arg1	locations					1324:1332	the different gut locations	1306:1332	the different gut locations of commercial pigs	1306:1351	These results should improve our understanding of microbiota compositions in the different gut locations of commercial pigs and provide important insights into the effect of gut microbiota on porcine FCRs.
29540768	2	57	theme	rRNA	471:474	arg1	sequencing					481:490	16S rRNA gene sequencing	467:490	16S rRNA gene sequencing	467:490	In this study, we performed a comparative analysis of the gut microbiome in the ileum, cecum, and colon of Duroc × (Landrace × Yorkshire) (DLY) pigs showing two extreme feed conversion ratios (FCRs) using 16S rRNA gene sequencing.
29540768	3	58	theme	higher	584:589	arg1	diversity					597:605	significantly higher alpha diversity	570:605	significantly higher alpha diversity	570:605	The results revealed that the microbial community in the cecum and colon had significantly higher alpha diversity than the ileum.
29540768	3	59	theme	microbial	523:531	arg1	community					533:541	the microbial community	519:541	the microbial community in the cecum and colon	519:564	The results revealed that the microbial community in the cecum and colon had significantly higher alpha diversity than the ileum.
29540768	2	60	theme	16S	467:469	arg1	sequencing					481:490	16S rRNA gene sequencing	467:490	16S rRNA gene sequencing	467:490	In this study, we performed a comparative analysis of the gut microbiome in the ileum, cecum, and colon of Duroc × (Landrace × Yorkshire) (DLY) pigs showing two extreme feed conversion ratios (FCRs) using 16S rRNA gene sequencing.
29540768	0	61	from	pigs	88:91	arg1	comparison					9:18	A global comparison	0:18	A global comparison of the microbiome compositions of three gut locations in commercial pigs with extreme feed conversion ratios.	0:128	A global comparison of the microbiome compositions of three gut locations in commercial pigs with extreme feed conversion ratios.
29540768	4	62	theme	taxonomic	672:680	arg1	OTUs					689:692	OTUs	689:692	OTUs	689:692	We further identified 11, 55, and 55 operational taxonomic units (OTUs) with significantly different relative abundances between the high and low FCR pigs among the three gut locations, respectively.
29540768	4	62	theme	taxonomic	672:680	arg1	units					682:686	11, 55, and 55 operational taxonomic units	645:686	11, 55, and 55 operational taxonomic units (OTUs) with significantly different relative abundances between the high and low FCR pigs	645:776	We further identified 11, 55, and 55 operational taxonomic units (OTUs) with significantly different relative abundances between the high and low FCR pigs among the three gut locations, respectively.
29540768	6	63	theme	high	1047:1050	arg1	pigs					1056:1059	the high FCR pigs	1043:1059	the high FCR pigs	1043:1059	We then identified two and nine metabolic pathways that were enriched in the cecum and colon of the high FCR pigs, respectively.
29540768	7	64	theme	indolic	1135:1141	arg1	compounds					1143:1151	indolic compounds	1135:1151	indolic compounds	1135:1151	The results suggested that the short chain fatty acids and indolic compounds produced by microbial fermentation might influence porcine feed efficiency.
29540768	0	65	theme	gut	60:62	arg1	locations					64:72	three gut locations	54:72	three gut locations	54:72	A global comparison of the microbiome compositions of three gut locations in commercial pigs with extreme feed conversion ratios.
29540768	2	66	theme	gut	320:322	arg1	microbiome					324:333	the gut microbiome	316:333	the gut microbiome	316:333	In this study, we performed a comparative analysis of the gut microbiome in the ileum, cecum, and colon of Duroc × (Landrace × Yorkshire) (DLY) pigs showing two extreme feed conversion ratios (FCRs) using 16S rRNA gene sequencing.
29540768	6	67	theme	two	966:968	arg1	pathways					989:996	two and nine metabolic pathways	966:996	pathways	989:996	We then identified two and nine metabolic pathways that were enriched in the cecum and colon of the high FCR pigs, respectively.
29540768	1	68	theme	gut	210:212	arg1	microbiome					214:223	the gut microbiome	206:223	the gut microbiome	206:223	In an attempt to increase profits and sustainability in the swine industry, the gut microbiome has become a focus of much research.
29540768	7	69	theme	short	1107:1111	arg1	acids					1125:1129	short chain fatty acids	1107:1129	short chain fatty acids	1107:1129	The results suggested that the short chain fatty acids and indolic compounds produced by microbial fermentation might influence porcine feed efficiency.
29540768	2	70	theme	conversion	436:445	arg1	FCRs					455:458	FCRs	455:458	FCRs	455:458	In this study, we performed a comparative analysis of the gut microbiome in the ileum, cecum, and colon of Duroc × (Landrace × Yorkshire) (DLY) pigs showing two extreme feed conversion ratios (FCRs) using 16S rRNA gene sequencing.
29540768	2	70	theme	conversion	436:445	arg1	ratios					447:452	two extreme feed conversion ratios	419:452	two extreme feed conversion ratios (FCRs)	419:459	In this study, we performed a comparative analysis of the gut microbiome in the ileum, cecum, and colon of Duroc × (Landrace × Yorkshire) (DLY) pigs showing two extreme feed conversion ratios (FCRs) using 16S rRNA gene sequencing.
31760871	0	0	theme	microbiota	80:89	arg1	roles					67:71	roles	67:71	Triclocarban exposure exaggerates colitis and colon tumorigenesis: roles of gut microbiota involved.	0:99	Triclocarban exposure exaggerates colitis and colon tumorigenesis: roles of gut microbiota involved.
31760871	5	1	theme	knockout-induced	797:812	arg1	colitis					814:820	interleukin 10 (IL-10) knockout-induced colitis	774:820	interleukin 10 (IL-10) knockout-induced colitis	774:820	Exposure to TCC increased dextran sodium sulfate (DSS)- and interleukin 10 (IL-10) knockout-induced colitis, and exaggerated azoxymethane (AOM)/DSS-induced colon tumorigenesis in mice.
31760871	1	2	theme	antimicrobial	137:149	arg1	Triclocarban					101:112	Triclocarban	101:112	Triclocarban (TCC)	101:118	Triclocarban (TCC) is a widely used antimicrobial ingredient in consumer products and is a ubiquitous contaminant in the environment.
31760871	1	2	theme	antimicrobial	137:149	arg1	ingredient					151:160	a widely used antimicrobial ingredient	123:160	a widely used antimicrobial ingredient in consumer products	123:181	Triclocarban (TCC) is a widely used antimicrobial ingredient in consumer products and is a ubiquitous contaminant in the environment.
31760871	0	3	theme	gut	76:78	arg1	microbiota					80:89	gut microbiota	76:89	gut microbiota involved	76:98	Triclocarban exposure exaggerates colitis and colon tumorigenesis: roles of gut microbiota involved.
31760871	6	4	theme	DSS-induced	1028:1038	arg1	colitis					1040:1046	DSS-induced colitis	1028:1046	DSS-induced colitis in mice lacking the microbiota	1028:1077	Regarding the mechanisms, TCC exposure reduced the diversity and altered the composition of gut microbiota and failed to promote DSS-induced colitis in mice lacking the microbiota, supporting that the presence of the microbiota is critical for the pro-colitis effects of TCC.
31760871	5	5	theme	colon	870:874	arg1	tumorigenesis					876:888	azoxymethane (AOM)/DSS-induced colon tumorigenesis	839:888	azoxymethane (AOM)/DSS-induced colon tumorigenesis in mice	839:896	Exposure to TCC increased dextran sodium sulfate (DSS)- and interleukin 10 (IL-10) knockout-induced colitis, and exaggerated azoxymethane (AOM)/DSS-induced colon tumorigenesis in mice.
31760871	1	6	from	ingredient	151:160	arg1	environment					222:232	the environment	218:232	the environment	218:232	Triclocarban (TCC) is a widely used antimicrobial ingredient in consumer products and is a ubiquitous contaminant in the environment.
31760871	1	6	from	ingredient	151:160	arg1	products					174:181	consumer products	165:181	consumer products	165:181	Triclocarban (TCC) is a widely used antimicrobial ingredient in consumer products and is a ubiquitous contaminant in the environment.
31760871	5	7	theme	/DSS-induced	857:868	arg1	tumorigenesis					876:888	azoxymethane (AOM)/DSS-induced colon tumorigenesis	839:888	azoxymethane (AOM)/DSS-induced colon tumorigenesis in mice	839:896	Exposure to TCC increased dextran sodium sulfate (DSS)- and interleukin 10 (IL-10) knockout-induced colitis, and exaggerated azoxymethane (AOM)/DSS-induced colon tumorigenesis in mice.
31760871	7	8	theme	colitis-associated	1256:1273	arg1	cancer					1281:1286	colitis-associated colon cancer	1256:1286	colitis-associated colon cancer	1256:1286	Together, these results support TCC could be a novel risk factor for colitis and colitis-associated colon cancer, and further regulatory policies on this compound could be needed.
31760871	6	9	theme	pro-colitis	1147:1157	arg1	effects					1159:1165	the pro-colitis effects	1143:1165	the pro-colitis effects of TCC	1143:1172	Regarding the mechanisms, TCC exposure reduced the diversity and altered the composition of gut microbiota and failed to promote DSS-induced colitis in mice lacking the microbiota, supporting that the presence of the microbiota is critical for the pro-colitis effects of TCC.
31760871	4	10	theme	gut	677:679	arg1	mechanisms					702:711	gut microbiota-dependent mechanisms	677:711	gut microbiota-dependent mechanisms	677:711	Here we show that exposure to low-dose TCC exaggerated the severity of colitis and exacerbated the development of colitis-associated colon tumorigenesis, via gut microbiota-dependent mechanisms.
31760871	4	11	theme	low-dose	549:556	arg1	TCC					558:560	low-dose TCC	549:560	low-dose TCC	549:560	Here we show that exposure to low-dose TCC exaggerated the severity of colitis and exacerbated the development of colitis-associated colon tumorigenesis, via gut microbiota-dependent mechanisms.
31760871	7	12	theme	colon	1275:1279	arg1	cancer					1281:1286	colitis-associated colon cancer	1256:1286	colitis-associated colon cancer	1256:1286	Together, these results support TCC could be a novel risk factor for colitis and colitis-associated colon cancer, and further regulatory policies on this compound could be needed.
31760871	5	13	from	tumorigenesis	876:888	arg1	mice					893:896	mice	893:896	mice	893:896	Exposure to TCC increased dextran sodium sulfate (DSS)- and interleukin 10 (IL-10) knockout-induced colitis, and exaggerated azoxymethane (AOM)/DSS-induced colon tumorigenesis in mice.
31760871	3	14	theme	better	392:397	arg1	understanding					399:411	A better understanding	390:411	A better understanding of its impact on human health	390:441	A better understanding of its impact on human health could lead to significant impact for public health and regulatory policies.
31760871	2	15	theme	personal	366:373	arg1	products					380:387	many other personal care products	355:387	many other personal care products	355:387	In 2016, the FDA removed TCC from over-the-counter handwashing products, but this compound is still approved for use in many other personal care products.
31760871	5	16	theme	dextran	740:746	arg1	DSS					764:766	dextran sodium sulfate (DSS)-	740:768	dextran sodium sulfate (DSS)-	740:768	Exposure to TCC increased dextran sodium sulfate (DSS)- and interleukin 10 (IL-10) knockout-induced colitis, and exaggerated azoxymethane (AOM)/DSS-induced colon tumorigenesis in mice.
31760871	3	17	theme	regulatory	498:507	arg1	policies					509:516	regulatory policies	498:516	regulatory policies	498:516	A better understanding of its impact on human health could lead to significant impact for public health and regulatory policies.
31760871	1	18	theme	consumer	165:172	arg1	products					174:181	consumer products	165:181	consumer products	165:181	Triclocarban (TCC) is a widely used antimicrobial ingredient in consumer products and is a ubiquitous contaminant in the environment.
31760871	0	19	theme	exposure	13:20	arg1	exaggerates					22:32	Triclocarban exposure exaggerates colitis and colon tumorigenesis	0:64	Triclocarban exposure exaggerates colitis and colon tumorigenesis: roles of gut microbiota involved.	0:99	Triclocarban exposure exaggerates colitis and colon tumorigenesis: roles of gut microbiota involved.
31760871	0	19	theme	exposure	13:20	arg1	colitis					34:40	colitis	34:40	colitis	34:40	Triclocarban exposure exaggerates colitis and colon tumorigenesis: roles of gut microbiota involved.
31760871	0	19	theme	exposure	13:20	arg1	tumorigenesis					52:64	colon tumorigenesis	46:64	colon tumorigenesis	46:64	Triclocarban exposure exaggerates colitis and colon tumorigenesis: roles of gut microbiota involved.
31760871	7	20	theme	risk	1228:1231	arg1	TCC					1207:1209	TCC	1207:1209	TCC	1207:1209	Together, these results support TCC could be a novel risk factor for colitis and colitis-associated colon cancer, and further regulatory policies on this compound could be needed.
31760871	7	20	theme	risk	1228:1231	arg1	factor					1233:1238	a novel risk factor	1220:1238	a novel risk factor for colitis and colitis-associated colon cancer	1220:1286	Together, these results support TCC could be a novel risk factor for colitis and colitis-associated colon cancer, and further regulatory policies on this compound could be needed.
31760871	2	21	theme	many	355:358	arg1	products					380:387	many other personal care products	355:387	many other personal care products	355:387	In 2016, the FDA removed TCC from over-the-counter handwashing products, but this compound is still approved for use in many other personal care products.
31760871	0	22	theme	Triclocarban	0:11	arg1	exaggerates					22:32	Triclocarban exposure exaggerates colitis and colon tumorigenesis	0:64	Triclocarban exposure exaggerates colitis and colon tumorigenesis: roles of gut microbiota involved.	0:99	Triclocarban exposure exaggerates colitis and colon tumorigenesis: roles of gut microbiota involved.
31760871	0	22	theme	Triclocarban	0:11	arg1	colitis					34:40	colitis	34:40	colitis	34:40	Triclocarban exposure exaggerates colitis and colon tumorigenesis: roles of gut microbiota involved.
31760871	0	22	theme	Triclocarban	0:11	arg1	tumorigenesis					52:64	colon tumorigenesis	46:64	colon tumorigenesis	46:64	Triclocarban exposure exaggerates colitis and colon tumorigenesis: roles of gut microbiota involved.
31760871	2	23	from	use	348:350	arg1	products					380:387	many other personal care products	355:387	many other personal care products	355:387	In 2016, the FDA removed TCC from over-the-counter handwashing products, but this compound is still approved for use in many other personal care products.
31760871	4	24	theme	colitis-associated	633:650	arg1	tumorigenesis					658:670	colitis-associated colon tumorigenesis	633:670	colitis-associated colon tumorigenesis	633:670	Here we show that exposure to low-dose TCC exaggerated the severity of colitis and exacerbated the development of colitis-associated colon tumorigenesis, via gut microbiota-dependent mechanisms.
31760871	7	25	theme	regulatory	1301:1310	arg1	policies					1312:1319	further regulatory policies	1293:1319	further regulatory policies on this compound	1293:1336	Together, these results support TCC could be a novel risk factor for colitis and colitis-associated colon cancer, and further regulatory policies on this compound could be needed.
31760871	7	26	from	policies	1312:1319	arg1	compound					1329:1336	this compound	1324:1336	this compound	1324:1336	Together, these results support TCC could be a novel risk factor for colitis and colitis-associated colon cancer, and further regulatory policies on this compound could be needed.
31760871	2	27	theme	handwashing	286:296	arg1	products					298:305	over-the-counter handwashing products	269:305	over-the-counter handwashing products	269:305	In 2016, the FDA removed TCC from over-the-counter handwashing products, but this compound is still approved for use in many other personal care products.
31760871	3	28	theme	human	430:434	arg1	health					436:441	human health	430:441	human health	430:441	A better understanding of its impact on human health could lead to significant impact for public health and regulatory policies.
31760871	2	29	theme	over-the-counter	269:284	arg1	products					298:305	over-the-counter handwashing products	269:305	over-the-counter handwashing products	269:305	In 2016, the FDA removed TCC from over-the-counter handwashing products, but this compound is still approved for use in many other personal care products.
31760871	4	30	theme	colitis	590:596	arg1	severity					578:585	the severity	574:585	the severity of colitis	574:596	Here we show that exposure to low-dose TCC exaggerated the severity of colitis and exacerbated the development of colitis-associated colon tumorigenesis, via gut microbiota-dependent mechanisms.
31760871	4	30	theme	colitis	590:596	arg1	development					618:628	exacerbated the development	602:628	exacerbated the development of colitis-associated colon tumorigenesis	602:670	Here we show that exposure to low-dose TCC exaggerated the severity of colitis and exacerbated the development of colitis-associated colon tumorigenesis, via gut microbiota-dependent mechanisms.
31760871	0	31	theme	colon	46:50	arg1	exaggerates					22:32	Triclocarban exposure exaggerates colitis and colon tumorigenesis	0:64	Triclocarban exposure exaggerates colitis and colon tumorigenesis: roles of gut microbiota involved.	0:99	Triclocarban exposure exaggerates colitis and colon tumorigenesis: roles of gut microbiota involved.
31760871	0	31	theme	colon	46:50	arg1	tumorigenesis					52:64	colon tumorigenesis	46:64	colon tumorigenesis	46:64	Triclocarban exposure exaggerates colitis and colon tumorigenesis: roles of gut microbiota involved.
31760871	4	32	theme	colon	652:656	arg1	tumorigenesis					658:670	colitis-associated colon tumorigenesis	633:670	colitis-associated colon tumorigenesis	633:670	Here we show that exposure to low-dose TCC exaggerated the severity of colitis and exacerbated the development of colitis-associated colon tumorigenesis, via gut microbiota-dependent mechanisms.
31760871	1	33	theme	ubiquitous	192:201	arg1	contaminant					203:213	a ubiquitous contaminant	190:213	a ubiquitous contaminant in the environment	190:232	Triclocarban (TCC) is a widely used antimicrobial ingredient in consumer products and is a ubiquitous contaminant in the environment.
31760871	3	34	theme	impact	420:425	arg1	understanding					399:411	A better understanding	390:411	A better understanding of its impact on human health	390:441	A better understanding of its impact on human health could lead to significant impact for public health and regulatory policies.
31760871	6	35	theme	TCC	925:927	arg1	exposure					929:936	TCC exposure	925:936	TCC exposure	925:936	Regarding the mechanisms, TCC exposure reduced the diversity and altered the composition of gut microbiota and failed to promote DSS-induced colitis in mice lacking the microbiota, supporting that the presence of the microbiota is critical for the pro-colitis effects of TCC.
31760871	3	36	from	impact	420:425	arg1	health					436:441	human health	430:441	human health	430:441	A better understanding of its impact on human health could lead to significant impact for public health and regulatory policies.
31760871	2	37	theme	care	375:378	arg1	products					380:387	many other personal care products	355:387	many other personal care products	355:387	In 2016, the FDA removed TCC from over-the-counter handwashing products, but this compound is still approved for use in many other personal care products.
31760871	1	38	from	contaminant	203:213	arg1	environment					222:232	the environment	218:232	the environment	218:232	Triclocarban (TCC) is a widely used antimicrobial ingredient in consumer products and is a ubiquitous contaminant in the environment.
31760871	1	38	from	contaminant	203:213	arg1	products					174:181	consumer products	165:181	consumer products	165:181	Triclocarban (TCC) is a widely used antimicrobial ingredient in consumer products and is a ubiquitous contaminant in the environment.
31760871	5	39	theme	interleukin	774:784	arg1	colitis					814:820	interleukin 10 (IL-10) knockout-induced colitis	774:820	interleukin 10 (IL-10) knockout-induced colitis	774:820	Exposure to TCC increased dextran sodium sulfate (DSS)- and interleukin 10 (IL-10) knockout-induced colitis, and exaggerated azoxymethane (AOM)/DSS-induced colon tumorigenesis in mice.
31760871	7	40	theme	further	1293:1299	arg1	policies					1312:1319	further regulatory policies	1293:1319	further regulatory policies on this compound	1293:1336	Together, these results support TCC could be a novel risk factor for colitis and colitis-associated colon cancer, and further regulatory policies on this compound could be needed.
31760871	3	41	theme	significant	457:467	arg1	impact					469:474	significant impact	457:474	significant impact for public health and regulatory policies	457:516	A better understanding of its impact on human health could lead to significant impact for public health and regulatory policies.
31760871	3	42	from	health	436:441	arg1	understanding					399:411	A better understanding	390:411	A better understanding of its impact on human health	390:441	A better understanding of its impact on human health could lead to significant impact for public health and regulatory policies.
31760871	7	43	theme	novel	1222:1226	arg1	TCC					1207:1209	TCC	1207:1209	TCC	1207:1209	Together, these results support TCC could be a novel risk factor for colitis and colitis-associated colon cancer, and further regulatory policies on this compound could be needed.
31760871	7	43	theme	novel	1222:1226	arg1	factor					1233:1238	a novel risk factor	1220:1238	a novel risk factor for colitis and colitis-associated colon cancer	1220:1286	Together, these results support TCC could be a novel risk factor for colitis and colitis-associated colon cancer, and further regulatory policies on this compound could be needed.
31760871	6	44	theme	microbiota	1116:1125	arg1	critical					1130:1137	critical	1130:1137	critical	1130:1137	Regarding the mechanisms, TCC exposure reduced the diversity and altered the composition of gut microbiota and failed to promote DSS-induced colitis in mice lacking the microbiota, supporting that the presence of the microbiota is critical for the pro-colitis effects of TCC.
31760871	6	44	theme	microbiota	1116:1125	arg1	presence					1100:1107	the presence	1096:1107	the presence of the microbiota	1096:1125	Regarding the mechanisms, TCC exposure reduced the diversity and altered the composition of gut microbiota and failed to promote DSS-induced colitis in mice lacking the microbiota, supporting that the presence of the microbiota is critical for the pro-colitis effects of TCC.
31760871	0	45	dep	exaggerates	22:32	arg1	exaggerates					22:32	Triclocarban exposure exaggerates colitis and colon tumorigenesis	0:64	Triclocarban exposure exaggerates colitis and colon tumorigenesis: roles of gut microbiota involved.	0:99	Triclocarban exposure exaggerates colitis and colon tumorigenesis: roles of gut microbiota involved.
31760871	0	45	dep	exaggerates	22:32	arg1	roles					67:71	roles	67:71	Triclocarban exposure exaggerates colitis and colon tumorigenesis: roles of gut microbiota involved.	0:99	Triclocarban exposure exaggerates colitis and colon tumorigenesis: roles of gut microbiota involved.
31760871	0	45	dep	exaggerates	22:32	arg1	colitis					34:40	colitis	34:40	colitis	34:40	Triclocarban exposure exaggerates colitis and colon tumorigenesis: roles of gut microbiota involved.
31760871	0	45	dep	exaggerates	22:32	arg1	tumorigenesis					52:64	colon tumorigenesis	46:64	colon tumorigenesis	46:64	Triclocarban exposure exaggerates colitis and colon tumorigenesis: roles of gut microbiota involved.
31760871	4	46	theme	tumorigenesis	658:670	arg1	severity					578:585	the severity	574:585	the severity of colitis	574:596	Here we show that exposure to low-dose TCC exaggerated the severity of colitis and exacerbated the development of colitis-associated colon tumorigenesis, via gut microbiota-dependent mechanisms.
31760871	4	46	theme	tumorigenesis	658:670	arg1	development					618:628	exacerbated the development	602:628	exacerbated the development of colitis-associated colon tumorigenesis	602:670	Here we show that exposure to low-dose TCC exaggerated the severity of colitis and exacerbated the development of colitis-associated colon tumorigenesis, via gut microbiota-dependent mechanisms.
31760871	6	47	theme	microbiota	995:1004	arg1	composition					976:986	the composition	972:986	the composition of gut microbiota	972:1004	Regarding the mechanisms, TCC exposure reduced the diversity and altered the composition of gut microbiota and failed to promote DSS-induced colitis in mice lacking the microbiota, supporting that the presence of the microbiota is critical for the pro-colitis effects of TCC.
31760871	5	48	theme	sodium	748:753	arg1	DSS					764:766	dextran sodium sulfate (DSS)-	740:768	dextran sodium sulfate (DSS)-	740:768	Exposure to TCC increased dextran sodium sulfate (DSS)- and interleukin 10 (IL-10) knockout-induced colitis, and exaggerated azoxymethane (AOM)/DSS-induced colon tumorigenesis in mice.
31760871	4	49	theme	exacerbated	602:612	arg1	development					618:628	exacerbated the development	602:628	exacerbated the development of colitis-associated colon tumorigenesis	602:670	Here we show that exposure to low-dose TCC exaggerated the severity of colitis and exacerbated the development of colitis-associated colon tumorigenesis, via gut microbiota-dependent mechanisms.
31760871	6	50	theme	TCC	1170:1172	arg1	effects					1159:1165	the pro-colitis effects	1143:1165	the pro-colitis effects of TCC	1143:1172	Regarding the mechanisms, TCC exposure reduced the diversity and altered the composition of gut microbiota and failed to promote DSS-induced colitis in mice lacking the microbiota, supporting that the presence of the microbiota is critical for the pro-colitis effects of TCC.
31760871	3	51	from	understanding	399:411	arg1	health					436:441	human health	430:441	human health	430:441	A better understanding of its impact on human health could lead to significant impact for public health and regulatory policies.
31760871	3	52	theme	public	480:485	arg1	health					487:492	public health	480:492	public health	480:492	A better understanding of its impact on human health could lead to significant impact for public health and regulatory policies.
31760871	2	53	theme	other	360:364	arg1	products					380:387	many other personal care products	355:387	many other personal care products	355:387	In 2016, the FDA removed TCC from over-the-counter handwashing products, but this compound is still approved for use in many other personal care products.
31760871	6	54	theme	gut	991:993	arg1	microbiota					995:1004	gut microbiota	991:1004	gut microbiota	991:1004	Regarding the mechanisms, TCC exposure reduced the diversity and altered the composition of gut microbiota and failed to promote DSS-induced colitis in mice lacking the microbiota, supporting that the presence of the microbiota is critical for the pro-colitis effects of TCC.
31760871	5	55	theme	sulfate	755:761	arg1	DSS					764:766	dextran sodium sulfate (DSS)-	740:768	dextran sodium sulfate (DSS)-	740:768	Exposure to TCC increased dextran sodium sulfate (DSS)- and interleukin 10 (IL-10) knockout-induced colitis, and exaggerated azoxymethane (AOM)/DSS-induced colon tumorigenesis in mice.
31760871	6	56	from	colitis	1040:1046	arg1	mice					1051:1054	mice	1051:1054	mice lacking the microbiota	1051:1077	Regarding the mechanisms, TCC exposure reduced the diversity and altered the composition of gut microbiota and failed to promote DSS-induced colitis in mice lacking the microbiota, supporting that the presence of the microbiota is critical for the pro-colitis effects of TCC.
31760871	1	57	theme	used	132:135	arg1	Triclocarban					101:112	Triclocarban	101:112	Triclocarban (TCC)	101:118	Triclocarban (TCC) is a widely used antimicrobial ingredient in consumer products and is a ubiquitous contaminant in the environment.
31760871	1	57	theme	used	132:135	arg1	ingredient					151:160	a widely used antimicrobial ingredient	123:160	a widely used antimicrobial ingredient in consumer products	123:181	Triclocarban (TCC) is a widely used antimicrobial ingredient in consumer products and is a ubiquitous contaminant in the environment.
31760871	4	58	theme	microbiota-dependent	681:700	arg1	mechanisms					702:711	gut microbiota-dependent mechanisms	677:711	gut microbiota-dependent mechanisms	677:711	Here we show that exposure to low-dose TCC exaggerated the severity of colitis and exacerbated the development of colitis-associated colon tumorigenesis, via gut microbiota-dependent mechanisms.
30600014	2	0	theme	striking	500:507	arg1	similarity					509:518	striking similarity as revealed by USANS/SANS experiments	500:556	striking similarity as revealed by USANS/SANS experiments	500:556	These two hydrogels have distinctly different melting and rheological properties, but the structure of their gel networks bears striking similarity as revealed by USANS/SANS experiments.
30600014	1	1	theme	SANS	345:348	arg1	techniques					360:369	SANS and USANS techniques	345:369	SANS and USANS techniques	345:369	The structures of two hydrogels formed by purified brush-like polysaccharides from Plantago ovata seed mucilage have been characterised from the nanometre to micrometre scale by using a combination of SANS and USANS techniques.
30600014	5	2	theme	polysaccharides	1388:1402	arg1	properties					1361:1370	rheological and structural properties	1334:1370	properties	1361:1370	By enzymatically cleaving off terminal arabinose residues from the side chains, we have demonstrated that composition of side-chains has a strong effect on intermolecular interactions, which, in turn, has a profound effect on rheological and structural properties of these unique polysaccharides.
30600014	4	3	from	variations	1061:1070	arg1	bonding					1099:1105	intermolecular hydrogen bonding	1075:1105	intermolecular hydrogen bonding	1075:1105	These results suggest that, in contrast to most gel-forming polysaccharides for which gelation depends on a structural transition, the rheological properties of Plantago ovata mucilage gels are dependent on variations in intermolecular hydrogen bonding.
30600014	3	4	theme	network	738:744	arg1	structure					746:754	the network structure	734:754	the network structure	734:754	Surprisingly, we find that the dramatic changes in the rheological properties induced by temperature or change in the solvent quality are accompanied by a small alteration of the network structure as inferred from scattering curves recorded above melting or in a chaotropic solvent (0.7 M KOD).
30600014	2	5	theme	different	408:416	arg1	properties					442:451	distinctly different melting and rheological properties	397:451	distinctly different melting and rheological properties	397:451	These two hydrogels have distinctly different melting and rheological properties, but the structure of their gel networks bears striking similarity as revealed by USANS/SANS experiments.
30600014	5	6	from	effect	1254:1259	arg1	interactions					1279:1290	intermolecular interactions	1264:1290	intermolecular interactions	1264:1290	By enzymatically cleaving off terminal arabinose residues from the side chains, we have demonstrated that composition of side-chains has a strong effect on intermolecular interactions, which, in turn, has a profound effect on rheological and structural properties of these unique polysaccharides.
30600014	5	7	from	chains	1180:1185	arg1	residues					1157:1164	terminal arabinose residues	1138:1164	terminal arabinose residues from the side chains	1138:1185	By enzymatically cleaving off terminal arabinose residues from the side chains, we have demonstrated that composition of side-chains has a strong effect on intermolecular interactions, which, in turn, has a profound effect on rheological and structural properties of these unique polysaccharides.
30600014	4	8	theme	intermolecular	1075:1088	arg1	bonding					1099:1105	intermolecular hydrogen bonding	1075:1105	intermolecular hydrogen bonding	1075:1105	These results suggest that, in contrast to most gel-forming polysaccharides for which gelation depends on a structural transition, the rheological properties of Plantago ovata mucilage gels are dependent on variations in intermolecular hydrogen bonding.
30600014	5	9	theme	side-chains	1229:1239	arg1	composition					1214:1224	composition	1214:1224	composition of side-chains	1214:1239	By enzymatically cleaving off terminal arabinose residues from the side chains, we have demonstrated that composition of side-chains has a strong effect on intermolecular interactions, which, in turn, has a profound effect on rheological and structural properties of these unique polysaccharides.
30600014	1	10	theme	USANS	354:358	arg1	techniques					360:369	SANS and USANS techniques	345:369	SANS and USANS techniques	345:369	The structures of two hydrogels formed by purified brush-like polysaccharides from Plantago ovata seed mucilage have been characterised from the nanometre to micrometre scale by using a combination of SANS and USANS techniques.
30600014	2	11	theme	USANS/SANS	535:544	arg1	experiments					546:556	USANS/SANS experiments	535:556	USANS/SANS experiments	535:556	These two hydrogels have distinctly different melting and rheological properties, but the structure of their gel networks bears striking similarity as revealed by USANS/SANS experiments.
30600014	1	12	theme	techniques	360:369	arg1	combination					330:340	a combination	328:340	a combination of SANS and USANS techniques	328:369	The structures of two hydrogels formed by purified brush-like polysaccharides from Plantago ovata seed mucilage have been characterised from the nanometre to micrometre scale by using a combination of SANS and USANS techniques.
30600014	4	13	theme	mucilage	1030:1037	arg1	gels					1039:1042	Plantago ovata mucilage gels	1015:1042	Plantago ovata mucilage gels	1015:1042	These results suggest that, in contrast to most gel-forming polysaccharides for which gelation depends on a structural transition, the rheological properties of Plantago ovata mucilage gels are dependent on variations in intermolecular hydrogen bonding.
30600014	5	14	theme	rheological	1334:1344	arg1	properties					1361:1370	rheological and structural properties	1334:1370	properties	1361:1370	By enzymatically cleaving off terminal arabinose residues from the side chains, we have demonstrated that composition of side-chains has a strong effect on intermolecular interactions, which, in turn, has a profound effect on rheological and structural properties of these unique polysaccharides.
30600014	4	15	theme	structural	962:971	arg1	transition					973:982	a structural transition	960:982	a structural transition	960:982	These results suggest that, in contrast to most gel-forming polysaccharides for which gelation depends on a structural transition, the rheological properties of Plantago ovata mucilage gels are dependent on variations in intermolecular hydrogen bonding.
30600014	3	16	theme	structure	746:754	arg1	alteration					720:729	a small alteration	712:729	a small alteration of the network structure	712:754	Surprisingly, we find that the dramatic changes in the rheological properties induced by temperature or change in the solvent quality are accompanied by a small alteration of the network structure as inferred from scattering curves recorded above melting or in a chaotropic solvent (0.7 M KOD).
30600014	1	17	theme	hydrogels	166:174	arg1	structures					148:157	The structures	144:157	The structures of two hydrogels formed by purified brush-like polysaccharides from Plantago ovata seed mucilage	144:254	The structures of two hydrogels formed by purified brush-like polysaccharides from Plantago ovata seed mucilage have been characterised from the nanometre to micrometre scale by using a combination of SANS and USANS techniques.
30600014	5	18	contain	has	1241:1243	arg2	effect					1254:1259	a strong effect	1245:1259	a strong effect	1245:1259	By enzymatically cleaving off terminal arabinose residues from the side chains, we have demonstrated that composition of side-chains has a strong effect on intermolecular interactions, which, in turn, has a profound effect on rheological and structural properties of these unique polysaccharides.
30600014	5	18	contain	has	1241:1243	arg1	composition					1214:1224	composition	1214:1224	composition of side-chains	1214:1239	By enzymatically cleaving off terminal arabinose residues from the side chains, we have demonstrated that composition of side-chains has a strong effect on intermolecular interactions, which, in turn, has a profound effect on rheological and structural properties of these unique polysaccharides.
30600014	3	19	theme	chaotropic	822:831	arg1	solvent					833:839	a chaotropic solvent	820:839	a chaotropic solvent (0.7 M KOD)	820:851	Surprisingly, we find that the dramatic changes in the rheological properties induced by temperature or change in the solvent quality are accompanied by a small alteration of the network structure as inferred from scattering curves recorded above melting or in a chaotropic solvent (0.7 M KOD).
30600014	3	19	theme	chaotropic	822:831	arg1	KOD					848:850	0.7 M KOD	842:850	0.7 M KOD	842:850	Surprisingly, we find that the dramatic changes in the rheological properties induced by temperature or change in the solvent quality are accompanied by a small alteration of the network structure as inferred from scattering curves recorded above melting or in a chaotropic solvent (0.7 M KOD).
30600014	5	20	theme	structural	1350:1359	arg1	properties					1361:1370	rheological and structural properties	1334:1370	properties	1361:1370	By enzymatically cleaving off terminal arabinose residues from the side chains, we have demonstrated that composition of side-chains has a strong effect on intermolecular interactions, which, in turn, has a profound effect on rheological and structural properties of these unique polysaccharides.
30600014	4	21	theme	gels	1039:1042	arg1	properties					1001:1010	the rheological properties	985:1010	the rheological properties of Plantago ovata mucilage gels	985:1042	These results suggest that, in contrast to most gel-forming polysaccharides for which gelation depends on a structural transition, the rheological properties of Plantago ovata mucilage gels are dependent on variations in intermolecular hydrogen bonding.
30600014	4	21	theme	gels	1039:1042	arg1	dependent					1048:1056	dependent	1048:1056	dependent	1048:1056	These results suggest that, in contrast to most gel-forming polysaccharides for which gelation depends on a structural transition, the rheological properties of Plantago ovata mucilage gels are dependent on variations in intermolecular hydrogen bonding.
30600014	5	22	theme	terminal	1138:1145	arg1	residues					1157:1164	terminal arabinose residues	1138:1164	terminal arabinose residues from the side chains	1138:1185	By enzymatically cleaving off terminal arabinose residues from the side chains, we have demonstrated that composition of side-chains has a strong effect on intermolecular interactions, which, in turn, has a profound effect on rheological and structural properties of these unique polysaccharides.
30600014	0	23	theme	Multi-scale	0:10	arg1	assembly					12:19	Multi-scale assembly	0:19	Multi-scale assembly of hydrogels formed by highly branched arabinoxylans from Plantago ovata seed mucilage	0:106	Multi-scale assembly of hydrogels formed by highly branched arabinoxylans from Plantago ovata seed mucilage studied by USANS/SANS and rheology.
30600014	4	24	from	dependent	1048:1056	arg1	contrast					885:892	contrast	885:892	contrast to most gel-forming polysaccharides for which gelation depends on a structural transition	885:982	These results suggest that, in contrast to most gel-forming polysaccharides for which gelation depends on a structural transition, the rheological properties of Plantago ovata mucilage gels are dependent on variations in intermolecular hydrogen bonding.
30600014	0	25	dep	Plantago	79:86	arg1	mucilage					99:106	seed mucilage	94:106	Plantago ovata seed mucilage	79:106	Multi-scale assembly of hydrogels formed by highly branched arabinoxylans from Plantago ovata seed mucilage studied by USANS/SANS and rheology.
30600014	0	25	dep	Plantago	79:86	arg1	ovata					88:92	Plantago ovata seed mucilage	79:106	Plantago ovata seed mucilage	79:106	Multi-scale assembly of hydrogels formed by highly branched arabinoxylans from Plantago ovata seed mucilage studied by USANS/SANS and rheology.
30600014	3	26	from	changes	599:605	arg1	properties					626:635	the rheological properties	610:635	the rheological properties induced by temperature or change in the solvent quality	610:691	Surprisingly, we find that the dramatic changes in the rheological properties induced by temperature or change in the solvent quality are accompanied by a small alteration of the network structure as inferred from scattering curves recorded above melting or in a chaotropic solvent (0.7 M KOD).
30600014	2	27	contain	have	392:395	arg2	properties					442:451	distinctly different melting and rheological properties	397:451	distinctly different melting and rheological properties	397:451	These two hydrogels have distinctly different melting and rheological properties, but the structure of their gel networks bears striking similarity as revealed by USANS/SANS experiments.
30600014	2	27	contain	have	392:395	arg1	hydrogels					382:390	These two hydrogels	372:390	These two hydrogels	372:390	These two hydrogels have distinctly different melting and rheological properties, but the structure of their gel networks bears striking similarity as revealed by USANS/SANS experiments.
30600014	0	28	theme	hydrogels	24:32	arg1	assembly					12:19	Multi-scale assembly	0:19	Multi-scale assembly of hydrogels formed by highly branched arabinoxylans from Plantago ovata seed mucilage	0:106	Multi-scale assembly of hydrogels formed by highly branched arabinoxylans from Plantago ovata seed mucilage studied by USANS/SANS and rheology.
30600014	3	29	theme	scattering	773:782	arg1	curves					784:789	scattering curves	773:789	scattering curves recorded above melting or in a chaotropic solvent (0.7 M KOD)	773:851	Surprisingly, we find that the dramatic changes in the rheological properties induced by temperature or change in the solvent quality are accompanied by a small alteration of the network structure as inferred from scattering curves recorded above melting or in a chaotropic solvent (0.7 M KOD).
30600014	4	30	theme	rheological	989:999	arg1	properties					1001:1010	the rheological properties	985:1010	the rheological properties of Plantago ovata mucilage gels	985:1042	These results suggest that, in contrast to most gel-forming polysaccharides for which gelation depends on a structural transition, the rheological properties of Plantago ovata mucilage gels are dependent on variations in intermolecular hydrogen bonding.
30600014	4	30	theme	rheological	989:999	arg1	dependent					1048:1056	dependent	1048:1056	dependent	1048:1056	These results suggest that, in contrast to most gel-forming polysaccharides for which gelation depends on a structural transition, the rheological properties of Plantago ovata mucilage gels are dependent on variations in intermolecular hydrogen bonding.
30600014	1	31	theme	brush-like	195:204	arg1	polysaccharides					206:220	purified brush-like polysaccharides	186:220	purified brush-like polysaccharides from Plantago ovata seed mucilage	186:254	The structures of two hydrogels formed by purified brush-like polysaccharides from Plantago ovata seed mucilage have been characterised from the nanometre to micrometre scale by using a combination of SANS and USANS techniques.
30600014	3	32	theme	dramatic	590:597	arg1	changes					599:605	the dramatic changes	586:605	the dramatic changes in the rheological properties induced by temperature or change in the solvent quality	586:691	Surprisingly, we find that the dramatic changes in the rheological properties induced by temperature or change in the solvent quality are accompanied by a small alteration of the network structure as inferred from scattering curves recorded above melting or in a chaotropic solvent (0.7 M KOD).
30600014	1	33	theme	micrometre	302:311	arg1	scale					313:317	micrometre scale	302:317	micrometre scale	302:317	The structures of two hydrogels formed by purified brush-like polysaccharides from Plantago ovata seed mucilage have been characterised from the nanometre to micrometre scale by using a combination of SANS and USANS techniques.
30600014	2	34	theme	rheological	430:440	arg1	properties					442:451	distinctly different melting and rheological properties	397:451	distinctly different melting and rheological properties	397:451	These two hydrogels have distinctly different melting and rheological properties, but the structure of their gel networks bears striking similarity as revealed by USANS/SANS experiments.
30600014	2	35	theme	networks	485:492	arg1	structure					462:470	the structure	458:470	the structure of their gel networks	458:492	These two hydrogels have distinctly different melting and rheological properties, but the structure of their gel networks bears striking similarity as revealed by USANS/SANS experiments.
30600014	5	36	dep	residues	1157:1164	arg1	off					1134:1136	off	1134:1136	off	1134:1136	By enzymatically cleaving off terminal arabinose residues from the side chains, we have demonstrated that composition of side-chains has a strong effect on intermolecular interactions, which, in turn, has a profound effect on rheological and structural properties of these unique polysaccharides.
30600014	0	37	from	Plantago	79:86	arg1	arabinoxylans					60:72	highly branched arabinoxylans	44:72	highly branched arabinoxylans from Plantago ovata seed mucilage	44:106	Multi-scale assembly of hydrogels formed by highly branched arabinoxylans from Plantago ovata seed mucilage studied by USANS/SANS and rheology.
30600014	5	38	contain	has	1309:1311	arg1	effect					1254:1259	a strong effect	1245:1259	a strong effect	1245:1259	By enzymatically cleaving off terminal arabinose residues from the side chains, we have demonstrated that composition of side-chains has a strong effect on intermolecular interactions, which, in turn, has a profound effect on rheological and structural properties of these unique polysaccharides.
30600014	5	38	contain	has	1309:1311	arg2	effect					1324:1329	a profound effect	1313:1329	a profound effect	1313:1329	By enzymatically cleaving off terminal arabinose residues from the side chains, we have demonstrated that composition of side-chains has a strong effect on intermolecular interactions, which, in turn, has a profound effect on rheological and structural properties of these unique polysaccharides.
30600014	1	39	theme	purified	186:193	arg1	polysaccharides					206:220	purified brush-like polysaccharides	186:220	purified brush-like polysaccharides from Plantago ovata seed mucilage	186:254	The structures of two hydrogels formed by purified brush-like polysaccharides from Plantago ovata seed mucilage have been characterised from the nanometre to micrometre scale by using a combination of SANS and USANS techniques.
30600014	2	40	theme	gel	481:483	arg1	networks					485:492	their gel networks	475:492	their gel networks	475:492	These two hydrogels have distinctly different melting and rheological properties, but the structure of their gel networks bears striking similarity as revealed by USANS/SANS experiments.
30600014	5	41	theme	side	1175:1178	arg1	chains					1180:1185	the side chains	1171:1185	the side chains	1171:1185	By enzymatically cleaving off terminal arabinose residues from the side chains, we have demonstrated that composition of side-chains has a strong effect on intermolecular interactions, which, in turn, has a profound effect on rheological and structural properties of these unique polysaccharides.
30600014	3	42	theme	rheological	614:624	arg1	properties					626:635	the rheological properties	610:635	the rheological properties induced by temperature or change in the solvent quality	610:691	Surprisingly, we find that the dramatic changes in the rheological properties induced by temperature or change in the solvent quality are accompanied by a small alteration of the network structure as inferred from scattering curves recorded above melting or in a chaotropic solvent (0.7 M KOD).
30600014	2	43	contain	bears	494:498	arg1	structure					462:470	the structure	458:470	the structure of their gel networks	458:492	These two hydrogels have distinctly different melting and rheological properties, but the structure of their gel networks bears striking similarity as revealed by USANS/SANS experiments.
30600014	2	43	contain	bears	494:498	arg2	similarity					509:518	striking similarity as revealed by USANS/SANS experiments	500:556	striking similarity as revealed by USANS/SANS experiments	500:556	These two hydrogels have distinctly different melting and rheological properties, but the structure of their gel networks bears striking similarity as revealed by USANS/SANS experiments.
30600014	2	44	theme	melting	418:424	arg1	properties					442:451	distinctly different melting and rheological properties	397:451	distinctly different melting and rheological properties	397:451	These two hydrogels have distinctly different melting and rheological properties, but the structure of their gel networks bears striking similarity as revealed by USANS/SANS experiments.
30600014	0	45	theme	branched	51:58	arg1	arabinoxylans					60:72	highly branched arabinoxylans	44:72	highly branched arabinoxylans from Plantago ovata seed mucilage	44:106	Multi-scale assembly of hydrogels formed by highly branched arabinoxylans from Plantago ovata seed mucilage studied by USANS/SANS and rheology.
30600014	1	46	from	Plantago	227:234	arg1	polysaccharides					206:220	purified brush-like polysaccharides	186:220	purified brush-like polysaccharides from Plantago ovata seed mucilage	186:254	The structures of two hydrogels formed by purified brush-like polysaccharides from Plantago ovata seed mucilage have been characterised from the nanometre to micrometre scale by using a combination of SANS and USANS techniques.
30600014	4	47	theme	Plantago	1015:1022	arg1	gels					1039:1042	Plantago ovata mucilage gels	1015:1042	Plantago ovata mucilage gels	1015:1042	These results suggest that, in contrast to most gel-forming polysaccharides for which gelation depends on a structural transition, the rheological properties of Plantago ovata mucilage gels are dependent on variations in intermolecular hydrogen bonding.
30600014	5	48	theme	arabinose	1147:1155	arg1	residues					1157:1164	terminal arabinose residues	1138:1164	terminal arabinose residues from the side chains	1138:1185	By enzymatically cleaving off terminal arabinose residues from the side chains, we have demonstrated that composition of side-chains has a strong effect on intermolecular interactions, which, in turn, has a profound effect on rheological and structural properties of these unique polysaccharides.
30600014	4	49	theme	gel-forming	902:912	arg1	polysaccharides					914:928	most gel-forming polysaccharides	897:928	most gel-forming polysaccharides for which gelation depends on a structural transition	897:982	These results suggest that, in contrast to most gel-forming polysaccharides for which gelation depends on a structural transition, the rheological properties of Plantago ovata mucilage gels are dependent on variations in intermolecular hydrogen bonding.
30600014	4	50	theme	hydrogen	1090:1097	arg1	bonding					1099:1105	intermolecular hydrogen bonding	1075:1105	intermolecular hydrogen bonding	1075:1105	These results suggest that, in contrast to most gel-forming polysaccharides for which gelation depends on a structural transition, the rheological properties of Plantago ovata mucilage gels are dependent on variations in intermolecular hydrogen bonding.
30600014	5	51	theme	strong	1247:1252	arg1	effect					1254:1259	a strong effect	1245:1259	a strong effect	1245:1259	By enzymatically cleaving off terminal arabinose residues from the side chains, we have demonstrated that composition of side-chains has a strong effect on intermolecular interactions, which, in turn, has a profound effect on rheological and structural properties of these unique polysaccharides.
30600014	4	52	theme	most	897:900	arg1	polysaccharides					914:928	most gel-forming polysaccharides	897:928	most gel-forming polysaccharides for which gelation depends on a structural transition	897:982	These results suggest that, in contrast to most gel-forming polysaccharides for which gelation depends on a structural transition, the rheological properties of Plantago ovata mucilage gels are dependent on variations in intermolecular hydrogen bonding.
30600014	3	53	theme	solvent	677:683	arg1	quality					685:691	the solvent quality	673:691	the solvent quality	673:691	Surprisingly, we find that the dramatic changes in the rheological properties induced by temperature or change in the solvent quality are accompanied by a small alteration of the network structure as inferred from scattering curves recorded above melting or in a chaotropic solvent (0.7 M KOD).
30600014	1	54	dep	Plantago	227:234	arg1	mucilage					247:254	seed mucilage	242:254	Plantago ovata seed mucilage	227:254	The structures of two hydrogels formed by purified brush-like polysaccharides from Plantago ovata seed mucilage have been characterised from the nanometre to micrometre scale by using a combination of SANS and USANS techniques.
30600014	1	54	dep	Plantago	227:234	arg1	ovata					236:240	Plantago ovata seed mucilage	227:254	Plantago ovata seed mucilage	227:254	The structures of two hydrogels formed by purified brush-like polysaccharides from Plantago ovata seed mucilage have been characterised from the nanometre to micrometre scale by using a combination of SANS and USANS techniques.
30600014	5	55	theme	profound	1315:1322	arg1	effect					1324:1329	a profound effect	1313:1329	a profound effect	1313:1329	By enzymatically cleaving off terminal arabinose residues from the side chains, we have demonstrated that composition of side-chains has a strong effect on intermolecular interactions, which, in turn, has a profound effect on rheological and structural properties of these unique polysaccharides.
30600014	1	56	theme	seed	242:245	arg1	mucilage					247:254	seed mucilage	242:254	Plantago ovata seed mucilage	227:254	The structures of two hydrogels formed by purified brush-like polysaccharides from Plantago ovata seed mucilage have been characterised from the nanometre to micrometre scale by using a combination of SANS and USANS techniques.
30600014	4	57	from	contrast	885:892	arg1	properties					1001:1010	the rheological properties	985:1010	the rheological properties of Plantago ovata mucilage gels	985:1042	These results suggest that, in contrast to most gel-forming polysaccharides for which gelation depends on a structural transition, the rheological properties of Plantago ovata mucilage gels are dependent on variations in intermolecular hydrogen bonding.
30600014	4	57	from	contrast	885:892	arg1	dependent					1048:1056	dependent	1048:1056	dependent	1048:1056	These results suggest that, in contrast to most gel-forming polysaccharides for which gelation depends on a structural transition, the rheological properties of Plantago ovata mucilage gels are dependent on variations in intermolecular hydrogen bonding.
30600014	4	58	theme	ovata	1024:1028	arg1	gels					1039:1042	Plantago ovata mucilage gels	1015:1042	Plantago ovata mucilage gels	1015:1042	These results suggest that, in contrast to most gel-forming polysaccharides for which gelation depends on a structural transition, the rheological properties of Plantago ovata mucilage gels are dependent on variations in intermolecular hydrogen bonding.
30600014	3	59	theme	small	714:718	arg1	alteration					720:729	a small alteration	712:729	a small alteration of the network structure	712:754	Surprisingly, we find that the dramatic changes in the rheological properties induced by temperature or change in the solvent quality are accompanied by a small alteration of the network structure as inferred from scattering curves recorded above melting or in a chaotropic solvent (0.7 M KOD).
30600014	5	60	theme	intermolecular	1264:1277	arg1	interactions					1279:1290	intermolecular interactions	1264:1290	intermolecular interactions	1264:1290	By enzymatically cleaving off terminal arabinose residues from the side chains, we have demonstrated that composition of side-chains has a strong effect on intermolecular interactions, which, in turn, has a profound effect on rheological and structural properties of these unique polysaccharides.
30600014	5	61	theme	unique	1381:1386	arg1	polysaccharides					1388:1402	these unique polysaccharides	1375:1402	these unique polysaccharides	1375:1402	By enzymatically cleaving off terminal arabinose residues from the side chains, we have demonstrated that composition of side-chains has a strong effect on intermolecular interactions, which, in turn, has a profound effect on rheological and structural properties of these unique polysaccharides.
30600014	0	62	theme	seed	94:97	arg1	mucilage					99:106	seed mucilage	94:106	Plantago ovata seed mucilage	79:106	Multi-scale assembly of hydrogels formed by highly branched arabinoxylans from Plantago ovata seed mucilage studied by USANS/SANS and rheology.
31089913	7	0	theme	Phylogenetic	577:588	arg1	analysis					590:597	Phylogenetic analysis	577:597	Phylogenetic analysis based on 16S rRNA gene sequences	577:630	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CBS5Q-3T is closely related to species of genus Jiella and shares high 16S rRNA gene sequence similarity of 98.1% with Jiella aquimaris JCM 30119T.
31089913	3	1	theme	Gram-negative	108:120	arg1	bacterium					162:170	A Gram-negative, aerobic, short rodshaped, asporogenous bacterium	106:170	A Gram-negative, aerobic, short rodshaped, asporogenous bacterium	106:170	A Gram-negative, aerobic, short rodshaped, asporogenous bacterium, designated CBS5Q-3T, was isolated from a surface-sterilised root of Ficus microcarpa Linn.
31089913	12	2	theme	name	1623:1626	arg1	sp					1647:1648	the name Jiella endophytica sp	1619:1648	the name Jiella endophytica sp	1619:1648	On the basis of phylogenetic, chemotaxonomic and phenotypic data, strain CBS5Q-3T can be concluded to represent a novel species of the genus Jiella, for which the name Jiella endophytica sp.
31089913	3	3	attach	isolated	198:205	arg2	bacterium					162:170	A Gram-negative, aerobic, short rodshaped, asporogenous bacterium	106:170	A Gram-negative, aerobic, short rodshaped, asporogenous bacterium	106:170	A Gram-negative, aerobic, short rodshaped, asporogenous bacterium, designated CBS5Q-3T, was isolated from a surface-sterilised root of Ficus microcarpa Linn.
31089913	3	3	attach	isolated	198:205	arg1	root					233:236	a surface-sterilised root	212:236	a surface-sterilised root of Ficus microcarpa Linn	212:261	A Gram-negative, aerobic, short rodshaped, asporogenous bacterium, designated CBS5Q-3T, was isolated from a surface-sterilised root of Ficus microcarpa Linn.
31089913	12	4	theme	strain	1526:1531	arg1	CBS5Q-3T					1533:1540	strain CBS5Q-3T	1526:1540	strain CBS5Q-3T	1526:1540	On the basis of phylogenetic, chemotaxonomic and phenotypic data, strain CBS5Q-3T can be concluded to represent a novel species of the genus Jiella, for which the name Jiella endophytica sp.
31089913	8	5	dep	in	835:836	arg1	silico					838:843	silico	838:843	silico	838:843	The average nucleotide identity and in silico DNA-DNA hybridization values between strain CBS5Q-3T and J. aquimaris JCM 30119T were 82.8% and 26.0%, respectively.
31089913	14	6	theme	type	1673:1676	arg1	CBS5Q-3T					1688:1695	CBS5Q-3T	1688:1695	CBS5Q-3T (= JCM 33167T = CGMCC 1.13863T)	1688:1727	The type strain is CBS5Q-3T (= JCM 33167T = CGMCC 1.13863T).
31089913	14	6	theme	type	1673:1676	arg1	strain					1678:1683	The type strain	1669:1683	The type strain	1669:1683	The type strain is CBS5Q-3T (= JCM 33167T = CGMCC 1.13863T).
31089913	6	7	theme	Substrate	461:469	arg1	mycelia					471:477	Substrate mycelia	461:477	Substrate mycelia	461:477	Substrate mycelia and aerial mycelia were not formed, and no diffusible pigments were observed on the media tested.
31089913	4	8	theme	taxonomic	356:364	arg1	position					366:373	its taxonomic position	352:373	its taxonomic position	352:373	f. collected from Guangxi, China and investigated by a polyphasic approach to determine its taxonomic position.
31089913	1	9	theme	endophytic	37:46	arg1	bacterium					48:56	a novel endophytic bacterium	29:56	a novel endophytic bacterium	29:56	nov., a novel endophytic bacterium isolated from root of Ficus microcarpa Linn.
31089913	1	9	theme	endophytic	37:46	arg1	nov.					23:26	nov.	23:26	nov.	23:26	nov., a novel endophytic bacterium isolated from root of Ficus microcarpa Linn.
31089913	7	10	theme	JCM	787:789	arg1	30119T					791:796	Jiella aquimaris JCM 30119T	770:796	Jiella aquimaris JCM 30119T	770:796	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CBS5Q-3T is closely related to species of genus Jiella and shares high 16S rRNA gene sequence similarity of 98.1% with Jiella aquimaris JCM 30119T.
31089913	10	11	theme	meso-diaminopimelic	1085:1103	arg1	acid					1105:1108	meso-diaminopimelic acid	1085:1108	meso-diaminopimelic acid	1085:1108	The cell wall peptidoglycan was found to contain meso-diaminopimelic acid and ubiquinone Q-10 identified as the respiratory lipoquinone.
31089913	12	12	theme	genus	1595:1599	arg1	Jiella					1601:1606	the genus Jiella	1591:1606	the genus Jiella	1591:1606	On the basis of phylogenetic, chemotaxonomic and phenotypic data, strain CBS5Q-3T can be concluded to represent a novel species of the genus Jiella, for which the name Jiella endophytica sp.
31089913	11	13	theme	polar	1177:1181	arg1	lipids					1183:1188	The polar lipids	1173:1188	The polar lipids	1173:1188	The polar lipids were found to be comprised of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylmonomethylethanolamine, phosphatidylethanolamine and three unidentified aminolipids, while the major fatty acids were identified as C18:1ω7c and cyclo-C19:0ω8c.
31089913	8	14	theme	aquimaris	905:913	arg1	30119T					919:924	J. aquimaris JCM 30119T	902:924	J. aquimaris JCM 30119T	902:924	The average nucleotide identity and in silico DNA-DNA hybridization values between strain CBS5Q-3T and J. aquimaris JCM 30119T were 82.8% and 26.0%, respectively.
31089913	8	15	theme	identity	822:829	arg1	values					867:872	The average nucleotide identity and in silico DNA-DNA hybridization values	799:872	The average nucleotide identity and in silico DNA-DNA hybridization values between strain CBS5Q-3T and J. aquimaris JCM 30119T	799:924	The average nucleotide identity and in silico DNA-DNA hybridization values between strain CBS5Q-3T and J. aquimaris JCM 30119T were 82.8% and 26.0%, respectively.
31089913	8	15	theme	identity	822:829	arg1	%					935:935	82.8%	931:935	82.8%	931:935	The average nucleotide identity and in silico DNA-DNA hybridization values between strain CBS5Q-3T and J. aquimaris JCM 30119T were 82.8% and 26.0%, respectively.
31089913	9	16	theme	DNA	966:968	arg1	content					976:982	The DNA G + C content	962:982	The DNA G + C content of strain CBS5Q-3T	962:1001	The DNA G + C content of strain CBS5Q-3T was determined to be 66.5 mol %.
31089913	9	16	theme	DNA	966:968	arg1	%					1033:1033	66.5 mol %	1024:1033	66.5 mol %	1024:1033	The DNA G + C content of strain CBS5Q-3T was determined to be 66.5 mol %.
31089913	7	17	theme	strain	644:649	arg1	CBS5Q-3T					651:658	strain CBS5Q-3T	644:658	strain CBS5Q-3T	644:658	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CBS5Q-3T is closely related to species of genus Jiella and shares high 16S rRNA gene sequence similarity of 98.1% with Jiella aquimaris JCM 30119T.
31089913	11	18	theme	unidentified	1358:1369	arg1	aminolipids					1371:1381	three unidentified aminolipids	1352:1381	three unidentified aminolipids	1352:1381	The polar lipids were found to be comprised of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylmonomethylethanolamine, phosphatidylethanolamine and three unidentified aminolipids, while the major fatty acids were identified as C18:1ω7c and cyclo-C19:0ω8c.
31089913	11	19	theme	fatty	1400:1404	arg1	acids					1406:1410	the major fatty acids	1390:1410	the major fatty acids	1390:1410	The polar lipids were found to be comprised of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylmonomethylethanolamine, phosphatidylethanolamine and three unidentified aminolipids, while the major fatty acids were identified as C18:1ω7c and cyclo-C19:0ω8c.
31089913	11	19	theme	fatty	1400:1404	arg1	cyclo-C19:0ω8c					1444:1457	cyclo-C19:0ω8c	1444:1457	cyclo-C19:0ω8c	1444:1457	The polar lipids were found to be comprised of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylmonomethylethanolamine, phosphatidylethanolamine and three unidentified aminolipids, while the major fatty acids were identified as C18:1ω7c and cyclo-C19:0ω8c.
31089913	11	19	theme	fatty	1400:1404	arg1	C18:1ω7c					1431:1438	C18:1ω7c	1431:1438	C18:1ω7c	1431:1438	The polar lipids were found to be comprised of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylmonomethylethanolamine, phosphatidylethanolamine and three unidentified aminolipids, while the major fatty acids were identified as C18:1ω7c and cyclo-C19:0ω8c.
31089913	3	20	theme	surface-sterilised	214:231	arg1	root					233:236	a surface-sterilised root	212:236	a surface-sterilised root of Ficus microcarpa Linn	212:261	A Gram-negative, aerobic, short rodshaped, asporogenous bacterium, designated CBS5Q-3T, was isolated from a surface-sterilised root of Ficus microcarpa Linn.
31089913	8	21	theme	J.	902:903	arg1	30119T					919:924	J. aquimaris JCM 30119T	902:924	J. aquimaris JCM 30119T	902:924	The average nucleotide identity and in silico DNA-DNA hybridization values between strain CBS5Q-3T and J. aquimaris JCM 30119T were 82.8% and 26.0%, respectively.
31089913	10	22	theme	wall	1045:1048	arg1	peptidoglycan					1050:1062	The cell wall peptidoglycan	1036:1062	The cell wall peptidoglycan	1036:1062	The cell wall peptidoglycan was found to contain meso-diaminopimelic acid and ubiquinone Q-10 identified as the respiratory lipoquinone.
31089913	7	23	theme	Jiella	770:775	arg1	30119T					791:796	Jiella aquimaris JCM 30119T	770:796	Jiella aquimaris JCM 30119T	770:796	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CBS5Q-3T is closely related to species of genus Jiella and shares high 16S rRNA gene sequence similarity of 98.1% with Jiella aquimaris JCM 30119T.
31089913	12	24	theme	chemotaxonomic	1490:1503	arg1	data					1520:1523	phylogenetic, chemotaxonomic and phenotypic data	1476:1523	phylogenetic, chemotaxonomic and phenotypic data	1476:1523	On the basis of phylogenetic, chemotaxonomic and phenotypic data, strain CBS5Q-3T can be concluded to represent a novel species of the genus Jiella, for which the name Jiella endophytica sp.
31089913	10	25	contain	contain	1077:1083	arg1	peptidoglycan					1050:1062	The cell wall peptidoglycan	1036:1062	The cell wall peptidoglycan	1036:1062	The cell wall peptidoglycan was found to contain meso-diaminopimelic acid and ubiquinone Q-10 identified as the respiratory lipoquinone.
31089913	10	25	contain	contain	1077:1083	arg2	acid					1105:1108	meso-diaminopimelic acid	1085:1108	meso-diaminopimelic acid	1085:1108	The cell wall peptidoglycan was found to contain meso-diaminopimelic acid and ubiquinone Q-10 identified as the respiratory lipoquinone.
31089913	10	25	contain	contain	1077:1083	arg2	Q-10					1125:1128	ubiquinone Q-10	1114:1128	ubiquinone Q-10	1114:1128	The cell wall peptidoglycan was found to contain meso-diaminopimelic acid and ubiquinone Q-10 identified as the respiratory lipoquinone.
31089913	6	26	theme	diffusible	522:531	arg1	pigments					533:540	no diffusible pigments	519:540	no diffusible pigments	519:540	Substrate mycelia and aerial mycelia were not formed, and no diffusible pigments were observed on the media tested.
31089913	0	27	theme	endophytica	7:17	arg1	sp					19:20	Jiella endophytica sp	0:20	Jiella endophytica sp.	0:21	Jiella endophytica sp.
31089913	12	28	theme	novel	1574:1578	arg1	species					1580:1586	a novel species	1572:1586	a novel species	1572:1586	On the basis of phylogenetic, chemotaxonomic and phenotypic data, strain CBS5Q-3T can be concluded to represent a novel species of the genus Jiella, for which the name Jiella endophytica sp.
31089913	8	29	theme	JCM	915:917	arg1	30119T					919:924	J. aquimaris JCM 30119T	902:924	J. aquimaris JCM 30119T	902:924	The average nucleotide identity and in silico DNA-DNA hybridization values between strain CBS5Q-3T and J. aquimaris JCM 30119T were 82.8% and 26.0%, respectively.
31089913	7	30	theme	%	763:763	arg1	similarity					745:754	high 16S rRNA gene sequence similarity	717:754	high 16S rRNA gene sequence similarity of 98.1%	717:763	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CBS5Q-3T is closely related to species of genus Jiella and shares high 16S rRNA gene sequence similarity of 98.1% with Jiella aquimaris JCM 30119T.
31089913	0	31	theme	Jiella	0:5	arg1	sp					19:20	Jiella endophytica sp	0:20	Jiella endophytica sp.	0:21	Jiella endophytica sp.
31089913	7	32	theme	16S	722:724	arg1	similarity					745:754	high 16S rRNA gene sequence similarity	717:754	high 16S rRNA gene sequence similarity of 98.1%	717:763	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CBS5Q-3T is closely related to species of genus Jiella and shares high 16S rRNA gene sequence similarity of 98.1% with Jiella aquimaris JCM 30119T.
31089913	12	33	dep	data	1520:1523	arg1	basis					1467:1471	basis	1467:1471	basis	1467:1471	On the basis of phylogenetic, chemotaxonomic and phenotypic data, strain CBS5Q-3T can be concluded to represent a novel species of the genus Jiella, for which the name Jiella endophytica sp.
31089913	12	33	dep	data	1520:1523	arg1	the					1463:1465	the	1463:1465	the	1463:1465	On the basis of phylogenetic, chemotaxonomic and phenotypic data, strain CBS5Q-3T can be concluded to represent a novel species of the genus Jiella, for which the name Jiella endophytica sp.
31089913	5	34	theme	%	426:426	arg1	NaCl					434:437	2% (w/v) NaCl	425:437	2% (w/v) NaCl	425:437	Strain CBS5Q-3T was found to grow optimally with 2% (w/v) NaCl at 30 °C, pH 7.0-8.0.
31089913	7	35	theme	rRNA	726:729	arg1	similarity					745:754	high 16S rRNA gene sequence similarity	717:754	high 16S rRNA gene sequence similarity of 98.1%	717:763	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CBS5Q-3T is closely related to species of genus Jiella and shares high 16S rRNA gene sequence similarity of 98.1% with Jiella aquimaris JCM 30119T.
31089913	3	36	dep	Gram-negative	108:120	arg1	rodshaped					138:146	rodshaped	138:146	rodshaped	138:146	A Gram-negative, aerobic, short rodshaped, asporogenous bacterium, designated CBS5Q-3T, was isolated from a surface-sterilised root of Ficus microcarpa Linn.
31089913	3	36	dep	Gram-negative	108:120	arg1	asporogenous					149:160	asporogenous	149:160	asporogenous	149:160	A Gram-negative, aerobic, short rodshaped, asporogenous bacterium, designated CBS5Q-3T, was isolated from a surface-sterilised root of Ficus microcarpa Linn.
31089913	3	36	dep	Gram-negative	108:120	arg1	aerobic					123:129	aerobic	123:129	aerobic	123:129	A Gram-negative, aerobic, short rodshaped, asporogenous bacterium, designated CBS5Q-3T, was isolated from a surface-sterilised root of Ficus microcarpa Linn.
31089913	3	37	theme	Linn	258:261	arg1	root					233:236	a surface-sterilised root	212:236	a surface-sterilised root of Ficus microcarpa Linn	212:261	A Gram-negative, aerobic, short rodshaped, asporogenous bacterium, designated CBS5Q-3T, was isolated from a surface-sterilised root of Ficus microcarpa Linn.
31089913	8	38	theme	nucleotide	811:820	arg1	identity					822:829	average nucleotide identity	803:829	average nucleotide identity	803:829	The average nucleotide identity and in silico DNA-DNA hybridization values between strain CBS5Q-3T and J. aquimaris JCM 30119T were 82.8% and 26.0%, respectively.
31089913	7	39	theme	gene	731:734	arg1	similarity					745:754	high 16S rRNA gene sequence similarity	717:754	high 16S rRNA gene sequence similarity of 98.1%	717:763	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CBS5Q-3T is closely related to species of genus Jiella and shares high 16S rRNA gene sequence similarity of 98.1% with Jiella aquimaris JCM 30119T.
31089913	8	40	theme	average	803:809	arg1	identity					822:829	average nucleotide identity	803:829	average nucleotide identity	803:829	The average nucleotide identity and in silico DNA-DNA hybridization values between strain CBS5Q-3T and J. aquimaris JCM 30119T were 82.8% and 26.0%, respectively.
31089913	7	41	theme	high	717:720	arg1	similarity					745:754	high 16S rRNA gene sequence similarity	717:754	high 16S rRNA gene sequence similarity of 98.1%	717:763	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CBS5Q-3T is closely related to species of genus Jiella and shares high 16S rRNA gene sequence similarity of 98.1% with Jiella aquimaris JCM 30119T.
31089913	10	42	theme	respiratory	1148:1158	arg1	lipoquinone					1160:1170	the respiratory lipoquinone	1144:1170	the respiratory lipoquinone	1144:1170	The cell wall peptidoglycan was found to contain meso-diaminopimelic acid and ubiquinone Q-10 identified as the respiratory lipoquinone.
31089913	7	43	theme	sequence	736:743	arg1	similarity					745:754	high 16S rRNA gene sequence similarity	717:754	high 16S rRNA gene sequence similarity of 98.1%	717:763	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CBS5Q-3T is closely related to species of genus Jiella and shares high 16S rRNA gene sequence similarity of 98.1% with Jiella aquimaris JCM 30119T.
31089913	7	44	theme	Jiella	699:704	arg1	species					682:688	species	682:688	species of genus Jiella	682:704	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CBS5Q-3T is closely related to species of genus Jiella and shares high 16S rRNA gene sequence similarity of 98.1% with Jiella aquimaris JCM 30119T.
31089913	6	45	located	observed	547:554	arg2	pigments					533:540	no diffusible pigments	519:540	no diffusible pigments	519:540	Substrate mycelia and aerial mycelia were not formed, and no diffusible pigments were observed on the media tested.
31089913	6	45	located	observed	547:554	arg1	media					563:567	the media	559:567	the media tested	559:574	Substrate mycelia and aerial mycelia were not formed, and no diffusible pigments were observed on the media tested.
31089913	7	46	theme	rRNA	612:615	arg1	sequences					622:630	16S rRNA gene sequences	608:630	16S rRNA gene sequences	608:630	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CBS5Q-3T is closely related to species of genus Jiella and shares high 16S rRNA gene sequence similarity of 98.1% with Jiella aquimaris JCM 30119T.
31089913	8	47	theme	in	835:836	arg1	hybridization					853:865	in silico DNA-DNA hybridization	835:865	in silico DNA-DNA hybridization	835:865	The average nucleotide identity and in silico DNA-DNA hybridization values between strain CBS5Q-3T and J. aquimaris JCM 30119T were 82.8% and 26.0%, respectively.
31089913	12	48	theme	Jiella	1601:1606	arg1	species					1580:1586	a novel species	1572:1586	a novel species	1572:1586	On the basis of phylogenetic, chemotaxonomic and phenotypic data, strain CBS5Q-3T can be concluded to represent a novel species of the genus Jiella, for which the name Jiella endophytica sp.
31089913	10	49	theme	cell	1040:1043	arg1	peptidoglycan					1050:1062	The cell wall peptidoglycan	1036:1062	The cell wall peptidoglycan	1036:1062	The cell wall peptidoglycan was found to contain meso-diaminopimelic acid and ubiquinone Q-10 identified as the respiratory lipoquinone.
31089913	7	50	theme	gene	617:620	arg1	sequences					622:630	16S rRNA gene sequences	608:630	16S rRNA gene sequences	608:630	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CBS5Q-3T is closely related to species of genus Jiella and shares high 16S rRNA gene sequence similarity of 98.1% with Jiella aquimaris JCM 30119T.
31089913	10	51	theme	ubiquinone	1114:1123	arg1	Q-10					1125:1128	ubiquinone Q-10	1114:1128	ubiquinone Q-10	1114:1128	The cell wall peptidoglycan was found to contain meso-diaminopimelic acid and ubiquinone Q-10 identified as the respiratory lipoquinone.
31089913	1	52	theme	Linn	97:100	arg1	root					72:75	root	72:75	root of Ficus microcarpa Linn	72:100	nov., a novel endophytic bacterium isolated from root of Ficus microcarpa Linn.
31089913	11	53	theme	major	1394:1398	arg1	acids					1406:1410	the major fatty acids	1390:1410	the major fatty acids	1390:1410	The polar lipids were found to be comprised of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylmonomethylethanolamine, phosphatidylethanolamine and three unidentified aminolipids, while the major fatty acids were identified as C18:1ω7c and cyclo-C19:0ω8c.
31089913	11	53	theme	major	1394:1398	arg1	cyclo-C19:0ω8c					1444:1457	cyclo-C19:0ω8c	1444:1457	cyclo-C19:0ω8c	1444:1457	The polar lipids were found to be comprised of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylmonomethylethanolamine, phosphatidylethanolamine and three unidentified aminolipids, while the major fatty acids were identified as C18:1ω7c and cyclo-C19:0ω8c.
31089913	11	53	theme	major	1394:1398	arg1	C18:1ω7c					1431:1438	C18:1ω7c	1431:1438	C18:1ω7c	1431:1438	The polar lipids were found to be comprised of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylmonomethylethanolamine, phosphatidylethanolamine and three unidentified aminolipids, while the major fatty acids were identified as C18:1ω7c and cyclo-C19:0ω8c.
31089913	4	54	theme	polyphasic	319:328	arg1	approach					330:337	a polyphasic approach	317:337	a polyphasic approach to determine its taxonomic position	317:373	f. collected from Guangxi, China and investigated by a polyphasic approach to determine its taxonomic position.
31089913	9	55	theme	G + C	970:974	arg1	content					976:982	The DNA G + C content	962:982	The DNA G + C content of strain CBS5Q-3T	962:1001	The DNA G + C content of strain CBS5Q-3T was determined to be 66.5 mol %.
31089913	9	55	theme	G + C	970:974	arg1	%					1033:1033	66.5 mol %	1024:1033	66.5 mol %	1024:1033	The DNA G + C content of strain CBS5Q-3T was determined to be 66.5 mol %.
31089913	14	56	theme	33167T = CGMCC	1704:1717	arg1	CBS5Q-3T					1688:1695	CBS5Q-3T	1688:1695	CBS5Q-3T (= JCM 33167T = CGMCC 1.13863T)	1688:1727	The type strain is CBS5Q-3T (= JCM 33167T = CGMCC 1.13863T).
31089913	14	56	theme	33167T = CGMCC	1704:1717	arg1	1.13863T					1719:1726	= JCM 33167T = CGMCC 1.13863T	1698:1726	= JCM 33167T = CGMCC 1.13863T	1698:1726	The type strain is CBS5Q-3T (= JCM 33167T = CGMCC 1.13863T).
31089913	7	57	theme	16S	608:610	arg1	sequences					622:630	16S rRNA gene sequences	608:630	16S rRNA gene sequences	608:630	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CBS5Q-3T is closely related to species of genus Jiella and shares high 16S rRNA gene sequence similarity of 98.1% with Jiella aquimaris JCM 30119T.
31089913	12	58	theme	phenotypic	1509:1518	arg1	data					1520:1523	phylogenetic, chemotaxonomic and phenotypic data	1476:1523	phylogenetic, chemotaxonomic and phenotypic data	1476:1523	On the basis of phylogenetic, chemotaxonomic and phenotypic data, strain CBS5Q-3T can be concluded to represent a novel species of the genus Jiella, for which the name Jiella endophytica sp.
31089913	7	59	dep	Jiella	770:775	arg1	aquimaris					777:785	aquimaris	777:785	aquimaris	777:785	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CBS5Q-3T is closely related to species of genus Jiella and shares high 16S rRNA gene sequence similarity of 98.1% with Jiella aquimaris JCM 30119T.
31089913	8	60	theme	strain	882:887	arg1	CBS5Q-3T					889:896	strain CBS5Q-3T	882:896	strain CBS5Q-3T	882:896	The average nucleotide identity and in silico DNA-DNA hybridization values between strain CBS5Q-3T and J. aquimaris JCM 30119T were 82.8% and 26.0%, respectively.
31089913	14	61	theme	= JCM	1698:1702	arg1	CBS5Q-3T					1688:1695	CBS5Q-3T	1688:1695	CBS5Q-3T (= JCM 33167T = CGMCC 1.13863T)	1688:1727	The type strain is CBS5Q-3T (= JCM 33167T = CGMCC 1.13863T).
31089913	14	61	theme	= JCM	1698:1702	arg1	1.13863T					1719:1726	= JCM 33167T = CGMCC 1.13863T	1698:1726	= JCM 33167T = CGMCC 1.13863T	1698:1726	The type strain is CBS5Q-3T (= JCM 33167T = CGMCC 1.13863T).
31089913	9	62	theme	strain	987:992	arg1	CBS5Q-3T					994:1001	strain CBS5Q-3T	987:1001	strain CBS5Q-3T	987:1001	The DNA G + C content of strain CBS5Q-3T was determined to be 66.5 mol %.
31089913	5	63	theme	pH	449:450	arg1	30 °C					442:446	30 °C	442:446	30 °C	442:446	Strain CBS5Q-3T was found to grow optimally with 2% (w/v) NaCl at 30 °C, pH 7.0-8.0.
31089913	5	63	theme	pH	449:450	arg1	7.0-8.0					452:458	pH 7.0-8.0	449:458	pH 7.0-8.0	449:458	Strain CBS5Q-3T was found to grow optimally with 2% (w/v) NaCl at 30 °C, pH 7.0-8.0.
31089913	9	64	theme	CBS5Q-3T	994:1001	arg1	content					976:982	The DNA G + C content	962:982	The DNA G + C content of strain CBS5Q-3T	962:1001	The DNA G + C content of strain CBS5Q-3T was determined to be 66.5 mol %.
31089913	9	64	theme	CBS5Q-3T	994:1001	arg1	%					1033:1033	66.5 mol %	1024:1033	66.5 mol %	1024:1033	The DNA G + C content of strain CBS5Q-3T was determined to be 66.5 mol %.
31089913	3	65	dep	rodshaped	138:146	arg1	short					132:136	short	132:136	short	132:136	A Gram-negative, aerobic, short rodshaped, asporogenous bacterium, designated CBS5Q-3T, was isolated from a surface-sterilised root of Ficus microcarpa Linn.
31089913	12	66	theme	endophytica	1635:1645	arg1	sp					1647:1648	the name Jiella endophytica sp	1619:1648	the name Jiella endophytica sp	1619:1648	On the basis of phylogenetic, chemotaxonomic and phenotypic data, strain CBS5Q-3T can be concluded to represent a novel species of the genus Jiella, for which the name Jiella endophytica sp.
31089913	8	67	theme	hybridization	853:865	arg1	values					867:872	The average nucleotide identity and in silico DNA-DNA hybridization values	799:872	The average nucleotide identity and in silico DNA-DNA hybridization values between strain CBS5Q-3T and J. aquimaris JCM 30119T	799:924	The average nucleotide identity and in silico DNA-DNA hybridization values between strain CBS5Q-3T and J. aquimaris JCM 30119T were 82.8% and 26.0%, respectively.
31089913	8	67	theme	hybridization	853:865	arg1	%					935:935	82.8%	931:935	82.8%	931:935	The average nucleotide identity and in silico DNA-DNA hybridization values between strain CBS5Q-3T and J. aquimaris JCM 30119T were 82.8% and 26.0%, respectively.
31089913	12	68	theme	phylogenetic	1476:1487	arg1	data					1520:1523	phylogenetic, chemotaxonomic and phenotypic data	1476:1523	phylogenetic, chemotaxonomic and phenotypic data	1476:1523	On the basis of phylogenetic, chemotaxonomic and phenotypic data, strain CBS5Q-3T can be concluded to represent a novel species of the genus Jiella, for which the name Jiella endophytica sp.
31089913	5	69	theme	2	425:425	arg1	%					426:426	%	426:426	%	426:426	Strain CBS5Q-3T was found to grow optimally with 2% (w/v) NaCl at 30 °C, pH 7.0-8.0.
31089913	6	70	theme	aerial	483:488	arg1	mycelia					490:496	aerial mycelia	483:496	aerial mycelia	483:496	Substrate mycelia and aerial mycelia were not formed, and no diffusible pigments were observed on the media tested.
31089913	1	71	theme	novel	31:35	arg1	bacterium					48:56	a novel endophytic bacterium	29:56	a novel endophytic bacterium	29:56	nov., a novel endophytic bacterium isolated from root of Ficus microcarpa Linn.
31089913	1	71	theme	novel	31:35	arg1	nov.					23:26	nov.	23:26	nov.	23:26	nov., a novel endophytic bacterium isolated from root of Ficus microcarpa Linn.
31089913	5	72	dep	%	426:426	arg1	w/v					429:431	w/v	429:431	w/v	429:431	Strain CBS5Q-3T was found to grow optimally with 2% (w/v) NaCl at 30 °C, pH 7.0-8.0.
31089913	8	73	theme	DNA-DNA	845:851	arg1	hybridization					853:865	in silico DNA-DNA hybridization	835:865	in silico DNA-DNA hybridization	835:865	The average nucleotide identity and in silico DNA-DNA hybridization values between strain CBS5Q-3T and J. aquimaris JCM 30119T were 82.8% and 26.0%, respectively.
31089913	12	74	theme	Jiella	1628:1633	arg1	sp					1647:1648	the name Jiella endophytica sp	1619:1648	the name Jiella endophytica sp	1619:1648	On the basis of phylogenetic, chemotaxonomic and phenotypic data, strain CBS5Q-3T can be concluded to represent a novel species of the genus Jiella, for which the name Jiella endophytica sp.
31089913	5	75	theme	Strain	376:381	arg1	CBS5Q-3T					383:390	Strain CBS5Q-3T	376:390	Strain CBS5Q-3T	376:390	Strain CBS5Q-3T was found to grow optimally with 2% (w/v) NaCl at 30 °C, pH 7.0-8.0.
30073131	17	0	theme	ATCC	2227:2230	arg1	8014					2232:2235	plantarum ATCC 8014	2217:2235	plantarum ATCC 8014	2217:2235	plantarum ATCC 8014 and Lb.
30073131	4	1	theme	thermal	577:583	arg1	properties					585:594	thermal properties	577:594	thermal properties	577:594	total carbohydrates, protein content, solubility rate, monosaccharides composition, structural information and thermal properties were also determined.
30073131	4	1	theme	thermal	577:583	arg1	carbohydrates					472:484	total carbohydrates	466:484	total carbohydrates	466:484	total carbohydrates, protein content, solubility rate, monosaccharides composition, structural information and thermal properties were also determined.
30073131	9	2	theme	NaOH-extracted	1077:1090	arg1	polysaccharides					1098:1112	NaOH-extracted crude polysaccharides	1077:1112	NaOH-extracted crude polysaccharides (8.73%)	1077:1120	There were significant differences (P < 0.05) between the values of NaOH-extracted crude polysaccharides (8.73%) and that of water (3.03%) and citric acid (3.07%)-extracted polysaccharides.
30073131	9	2	theme	NaOH-extracted	1077:1090	arg1	%					1119:1119	8.73%	1115:1119	8.73%	1115:1119	There were significant differences (P < 0.05) between the values of NaOH-extracted crude polysaccharides (8.73%) and that of water (3.03%) and citric acid (3.07%)-extracted polysaccharides.
30073131	2	3	theme	crude	317:321	arg1	polysaccharide					323:336	crude polysaccharide	317:336	crude polysaccharide	317:336	The PKC was defatted and crude polysaccharide was obtained through water, citric acid or NaOH extraction.
30073131	1	4	theme	palm	253:256	arg1	PKC					271:273	PKC	271:273	PKC	271:273	This paper deliberates the extraction, characterization and examination of potential application of soluble polysaccharides of palm kernel cake (PKC) as a prebiotic.
30073131	1	4	theme	palm	253:256	arg1	cake					265:268	palm kernel cake	253:268	palm kernel cake (PKC)	253:274	This paper deliberates the extraction, characterization and examination of potential application of soluble polysaccharides of palm kernel cake (PKC) as a prebiotic.
30073131	14	5	theme	degradation	1928:1938	arg1	temperature					1940:1950	the main degradation temperature	1919:1950	the main degradation temperature of SP is about 121 to 125 °C	1919:1979	FTIR spectroscopy revealed that the polysaccharides extracts were linked to β and α-glycosidic bonds and thermal analysis using differential scanning calorimeter (DSC) showed the main degradation temperature of SP is about 121 to 125 °C.
30073131	9	6	theme	polysaccharides	1098:1112	arg1	values					1067:1072	the values	1063:1072	the values of NaOH-extracted crude polysaccharides (8.73%)	1063:1120	There were significant differences (P < 0.05) between the values of NaOH-extracted crude polysaccharides (8.73%) and that of water (3.03%) and citric acid (3.07%)-extracted polysaccharides.
30073131	1	7	theme	cake	265:268	arg1	polysaccharides					234:248	soluble polysaccharides	226:248	soluble polysaccharides of palm kernel cake (PKC)	226:274	This paper deliberates the extraction, characterization and examination of potential application of soluble polysaccharides of palm kernel cake (PKC) as a prebiotic.
30073131	13	8	theme	Protein	1559:1565	arg1	content					1567:1573	Protein content	1559:1573	Protein content in SCPW, SCPCA and SCPN	1559:1597	Protein content in SCPW, SCPCA and SCPN are 0.72, 0.40 and 0.58, respectively, and the peaks which indicated the presence of protein were observed at approximately 1640 cm-1 (amide I).
30073131	5	9	theme	soluble	632:638	arg1	polysaccharides					640:654	The extracted soluble polysaccharides	618:654	The extracted soluble polysaccharides	618:654	The extracted soluble polysaccharides were further subjected to a digestibility test using artificial human gastric juice.
30073131	6	10	theme	plantarum	816:824	arg1	ATCC					826:829	Lactobacillus plantarum ATCC 8014	802:834	Lactobacillus plantarum ATCC 8014	802:834	Finally, their prebiotic potential on two probiotics, namely Lactobacillus plantarum ATCC 8014 and Lb.
30073131	6	10	theme	plantarum	816:824	arg1	probiotics					783:792	two probiotics	779:792	two probiotics	779:792	Finally, their prebiotic potential on two probiotics, namely Lactobacillus plantarum ATCC 8014 and Lb.
30073131	14	11	dep	125 °C	1974:1979	arg1	to					1971:1972	to	1971:1972	to	1971:1972	FTIR spectroscopy revealed that the polysaccharides extracts were linked to β and α-glycosidic bonds and thermal analysis using differential scanning calorimeter (DSC) showed the main degradation temperature of SP is about 121 to 125 °C.
30073131	18	12	theme	rhamnosus	2245:2253	arg1	ATCC					2255:2258	rhamnosus ATCC 53103	2245:2264	rhamnosus ATCC 53103	2245:2264	rhamnosus ATCC 53103 with decrease in the pH of the medium and producing organic acids.All the above findings strongly indicated that polysaccharides extracted from PKC, an industrial waste, have a potential to be exploited as novel prebiotics.
30073131	11	13	theme	Total	1379:1383	arg1	content					1398:1404	Total carbohydrate content	1379:1404	Total carbohydrate content in SCPW, SCPCA and SCPN	1379:1428	Total carbohydrate content in SCPW, SCPCA and SCPN are 57.11%, 56.94% and 50.95%, respectively.
30073131	11	13	theme	Total	1379:1383	arg1	%					1439:1439	57.11%	1434:1439	57.11%	1434:1439	Total carbohydrate content in SCPW, SCPCA and SCPN are 57.11%, 56.94% and 50.95%, respectively.
30073131	18	14	theme	industrial	2418:2427	arg1	PKC					2410:2412	PKC	2410:2412	PKC	2410:2412	rhamnosus ATCC 53103 with decrease in the pH of the medium and producing organic acids.All the above findings strongly indicated that polysaccharides extracted from PKC, an industrial waste, have a potential to be exploited as novel prebiotics.
30073131	18	14	theme	industrial	2418:2427	arg1	waste					2429:2433	an industrial waste	2415:2433	an industrial waste	2415:2433	rhamnosus ATCC 53103 with decrease in the pH of the medium and producing organic acids.All the above findings strongly indicated that polysaccharides extracted from PKC, an industrial waste, have a potential to be exploited as novel prebiotics.
30073131	13	15	theme	protein	1684:1690	arg1	presence					1672:1679	the presence	1668:1679	the presence of protein	1668:1690	Protein content in SCPW, SCPCA and SCPN are 0.72, 0.40 and 0.58, respectively, and the peaks which indicated the presence of protein were observed at approximately 1640 cm-1 (amide I).
30073131	5	16	theme	gastric	726:732	arg1	juice					734:738	artificial human gastric juice	709:738	artificial human gastric juice	709:738	The extracted soluble polysaccharides were further subjected to a digestibility test using artificial human gastric juice.
30073131	15	17	theme	human	2075:2079	arg1	juice					2089:2093	artificial human gastric juice	2064:2093	artificial human gastric juice	2064:2093	The SP were found to be highly resistance (> 96%) to hydrolysis when subjected to artificial human gastric juice.
30073131	14	18	theme	scanning	1885:1892	arg1	DSC					1907:1909	DSC	1907:1909	DSC	1907:1909	FTIR spectroscopy revealed that the polysaccharides extracts were linked to β and α-glycosidic bonds and thermal analysis using differential scanning calorimeter (DSC) showed the main degradation temperature of SP is about 121 to 125 °C.
30073131	14	18	theme	scanning	1885:1892	arg1	calorimeter					1894:1904	differential scanning calorimeter	1872:1904	differential scanning calorimeter (DSC)	1872:1910	FTIR spectroscopy revealed that the polysaccharides extracts were linked to β and α-glycosidic bonds and thermal analysis using differential scanning calorimeter (DSC) showed the main degradation temperature of SP is about 121 to 125 °C.
30073131	16	19	from	probiotics	2148:2157	arg1	potentials					2111:2120	The prebiotics potentials	2096:2120	The prebiotics potentials of the polysaccharides on probiotics	2096:2157	The prebiotics potentials of the polysaccharides on probiotics in vitro demonstrated an increase in proliferation of Lb.
30073131	1	20	theme	potential	201:209	arg1	application					211:221	potential application	201:221	potential application of soluble polysaccharides of palm kernel cake (PKC)	201:274	This paper deliberates the extraction, characterization and examination of potential application of soluble polysaccharides of palm kernel cake (PKC) as a prebiotic.
30073131	10	21	from	percentage	1316:1325	arg1	SPCA					1373:1376	SPCA	1373:1376	SPCA	1373:1376	The extracted polysaccharides composed of mannose, galactose, glucose, arabinose, xylose and rhamanose, with highest percentage of mannose (62.49%) and galactose (25.42%) in SPCA.
30073131	5	22	theme	artificial	709:718	arg1	juice					734:738	artificial human gastric juice	709:738	artificial human gastric juice	709:738	The extracted soluble polysaccharides were further subjected to a digestibility test using artificial human gastric juice.
30073131	13	23	from	content	1567:1573	arg1	SCPN					1594:1597	SCPN	1594:1597	SCPN	1594:1597	Protein content in SCPW, SCPCA and SCPN are 0.72, 0.40 and 0.58, respectively, and the peaks which indicated the presence of protein were observed at approximately 1640 cm-1 (amide I).
30073131	13	23	from	content	1567:1573	arg1	SCPCA					1584:1588	SCPCA	1584:1588	SCPCA	1584:1588	Protein content in SCPW, SCPCA and SCPN are 0.72, 0.40 and 0.58, respectively, and the peaks which indicated the presence of protein were observed at approximately 1640 cm-1 (amide I).
30073131	13	23	from	content	1567:1573	arg1	SCPW					1578:1581	SCPW	1578:1581	SCPW	1578:1581	Protein content in SCPW, SCPCA and SCPN are 0.72, 0.40 and 0.58, respectively, and the peaks which indicated the presence of protein were observed at approximately 1640 cm-1 (amide I).
30073131	0	24	theme	palm	55:58	arg1	kernel					60:65	palm kernel	55:65	palm kernel cake as a potential prebiotic	55:95	Evaluation of the effect of soluble polysaccharides of palm kernel cake as a potential prebiotic on the growth of probiotics.
30073131	9	25	theme	citric	1152:1157	arg1	acid					1159:1162	citric acid	1152:1162	citric acid (3.07%)	1152:1170	There were significant differences (P < 0.05) between the values of NaOH-extracted crude polysaccharides (8.73%) and that of water (3.03%) and citric acid (3.07%)-extracted polysaccharides.
30073131	9	25	theme	citric	1152:1157	arg1	%					1169:1169	3.07%	1165:1169	3.07%	1165:1169	There were significant differences (P < 0.05) between the values of NaOH-extracted crude polysaccharides (8.73%) and that of water (3.03%) and citric acid (3.07%)-extracted polysaccharides.
30073131	13	26	located	observed	1697:1704	arg2	peaks					1646:1650	the peaks	1642:1650	the peaks which indicated the presence of protein	1642:1690	Protein content in SCPW, SCPCA and SCPN are 0.72, 0.40 and 0.58, respectively, and the peaks which indicated the presence of protein were observed at approximately 1640 cm-1 (amide I).
30073131	13	26	located	observed	1697:1704	arg1	1640 cm-1					1723:1731	1640 cm-1	1723:1731	1640 cm-1	1723:1731	Protein content in SCPW, SCPCA and SCPN are 0.72, 0.40 and 0.58, respectively, and the peaks which indicated the presence of protein were observed at approximately 1640 cm-1 (amide I).
30073131	13	26	located	observed	1697:1704	arg1	I					1740:1740	amide I	1734:1740	amide I	1734:1740	Protein content in SCPW, SCPCA and SCPN are 0.72, 0.40 and 0.58, respectively, and the peaks which indicated the presence of protein were observed at approximately 1640 cm-1 (amide I).
30073131	10	27	theme	highest	1308:1314	arg1	percentage					1316:1325	highest percentage	1308:1325	highest percentage of mannose (62.49%) and galactose (25.42%) in SPCA	1308:1376	The extracted polysaccharides composed of mannose, galactose, glucose, arabinose, xylose and rhamanose, with highest percentage of mannose (62.49%) and galactose (25.42%) in SPCA.
30073131	14	28	dep	SP	1955:1956	arg1	125 °C					1974:1979	125 °C	1974:1979	125 °C	1974:1979	FTIR spectroscopy revealed that the polysaccharides extracts were linked to β and α-glycosidic bonds and thermal analysis using differential scanning calorimeter (DSC) showed the main degradation temperature of SP is about 121 to 125 °C.
30073131	0	29	from	Evaluation	0:9	arg1	growth					104:109	the growth	100:109	the growth of probiotics	100:123	Evaluation of the effect of soluble polysaccharides of palm kernel cake as a potential prebiotic on the growth of probiotics.
30073131	14	30	theme	α-glycosidic	1826:1837	arg1	bonds					1839:1843	β and α-glycosidic bonds	1820:1843	β and α-glycosidic bonds	1820:1843	FTIR spectroscopy revealed that the polysaccharides extracts were linked to β and α-glycosidic bonds and thermal analysis using differential scanning calorimeter (DSC) showed the main degradation temperature of SP is about 121 to 125 °C.
30073131	2	31	theme	NaOH	381:384	arg1	extraction					386:395	NaOH extraction	381:395	NaOH extraction	381:395	The PKC was defatted and crude polysaccharide was obtained through water, citric acid or NaOH extraction.
30073131	18	32	theme	above	2340:2344	arg1	findings					2346:2353	the above findings	2336:2353	the above findings	2336:2353	rhamnosus ATCC 53103 with decrease in the pH of the medium and producing organic acids.All the above findings strongly indicated that polysaccharides extracted from PKC, an industrial waste, have a potential to be exploited as novel prebiotics.
30073131	2	33	theme	citric	366:371	arg1	acid					373:376	citric acid	366:376	citric acid	366:376	The PKC was defatted and crude polysaccharide was obtained through water, citric acid or NaOH extraction.
30073131	9	34	theme	-extracted	1171:1180	arg1	polysaccharides					1182:1196	-extracted polysaccharides	1171:1196	-extracted polysaccharides	1171:1196	There were significant differences (P < 0.05) between the values of NaOH-extracted crude polysaccharides (8.73%) and that of water (3.03%) and citric acid (3.07%)-extracted polysaccharides.
30073131	4	35	theme	total	466:470	arg1	properties					585:594	thermal properties	577:594	thermal properties	577:594	total carbohydrates, protein content, solubility rate, monosaccharides composition, structural information and thermal properties were also determined.
30073131	4	35	theme	total	466:470	arg1	content					495:501	protein content	487:501	protein content	487:501	total carbohydrates, protein content, solubility rate, monosaccharides composition, structural information and thermal properties were also determined.
30073131	4	35	theme	total	466:470	arg1	composition					537:547	monosaccharides composition	521:547	monosaccharides composition	521:547	total carbohydrates, protein content, solubility rate, monosaccharides composition, structural information and thermal properties were also determined.
30073131	4	35	theme	total	466:470	arg1	information					561:571	structural information	550:571	structural information	550:571	total carbohydrates, protein content, solubility rate, monosaccharides composition, structural information and thermal properties were also determined.
30073131	4	35	theme	total	466:470	arg1	carbohydrates					472:484	total carbohydrates	466:484	total carbohydrates	466:484	total carbohydrates, protein content, solubility rate, monosaccharides composition, structural information and thermal properties were also determined.
30073131	4	35	theme	total	466:470	arg1	rate					515:518	solubility rate	504:518	solubility rate	504:518	total carbohydrates, protein content, solubility rate, monosaccharides composition, structural information and thermal properties were also determined.
30073131	14	36	theme	β	1820:1820	arg1	bonds					1839:1843	β and α-glycosidic bonds	1820:1843	β and α-glycosidic bonds	1820:1843	FTIR spectroscopy revealed that the polysaccharides extracts were linked to β and α-glycosidic bonds and thermal analysis using differential scanning calorimeter (DSC) showed the main degradation temperature of SP is about 121 to 125 °C.
30073131	18	37	theme	acids.All	2326:2334	arg1	pH					2287:2288	the pH	2283:2288	the pH of the medium and producing organic acids.All	2283:2334	rhamnosus ATCC 53103 with decrease in the pH of the medium and producing organic acids.All the above findings strongly indicated that polysaccharides extracted from PKC, an industrial waste, have a potential to be exploited as novel prebiotics.
30073131	3	38	theme	extracted	435:443	arg1	polysaccharides					445:459	the extracted polysaccharides	431:459	the extracted polysaccharides	431:459	The physiochemical properties of the extracted polysaccharides viz.
30073131	4	39	theme	structural	550:559	arg1	carbohydrates					472:484	total carbohydrates	466:484	total carbohydrates	466:484	total carbohydrates, protein content, solubility rate, monosaccharides composition, structural information and thermal properties were also determined.
30073131	4	39	theme	structural	550:559	arg1	information					561:571	structural information	550:571	structural information	550:571	total carbohydrates, protein content, solubility rate, monosaccharides composition, structural information and thermal properties were also determined.
30073131	9	40	theme	significant	1020:1030	arg1	P < 0.05					1045:1052	P < 0.05	1045:1052	P < 0.05	1045:1052	There were significant differences (P < 0.05) between the values of NaOH-extracted crude polysaccharides (8.73%) and that of water (3.03%) and citric acid (3.07%)-extracted polysaccharides.
30073131	9	40	theme	significant	1020:1030	arg1	differences					1032:1042	significant differences	1020:1042	significant differences (P < 0.05) between the values of NaOH-extracted crude polysaccharides (8.73%)	1020:1120	There were significant differences (P < 0.05) between the values of NaOH-extracted crude polysaccharides (8.73%) and that of water (3.03%) and citric acid (3.07%)-extracted polysaccharides.
30073131	16	41	from	increase	2184:2191	arg1	proliferation					2196:2208	proliferation	2196:2208	proliferation of Lb	2196:2214	The prebiotics potentials of the polysaccharides on probiotics in vitro demonstrated an increase in proliferation of Lb.
30073131	18	42	theme	medium	2297:2302	arg1	pH					2287:2288	the pH	2283:2288	the pH of the medium and producing organic acids.All	2283:2334	rhamnosus ATCC 53103 with decrease in the pH of the medium and producing organic acids.All the above findings strongly indicated that polysaccharides extracted from PKC, an industrial waste, have a potential to be exploited as novel prebiotics.
30073131	4	43	theme	solubility	504:513	arg1	carbohydrates					472:484	total carbohydrates	466:484	total carbohydrates	466:484	total carbohydrates, protein content, solubility rate, monosaccharides composition, structural information and thermal properties were also determined.
30073131	4	43	theme	solubility	504:513	arg1	rate					515:518	solubility rate	504:518	solubility rate	504:518	total carbohydrates, protein content, solubility rate, monosaccharides composition, structural information and thermal properties were also determined.
30073131	10	44	theme	extracted	1203:1211	arg1	polysaccharides					1213:1227	The extracted polysaccharides	1199:1227	The extracted polysaccharides	1199:1227	The extracted polysaccharides composed of mannose, galactose, glucose, arabinose, xylose and rhamanose, with highest percentage of mannose (62.49%) and galactose (25.42%) in SPCA.
30073131	0	45	theme	polysaccharides	36:50	arg1	effect					18:23	the effect	14:23	the effect of soluble polysaccharides of palm kernel cake as a potential prebiotic on the growth of probiotics	14:123	Evaluation of the effect of soluble polysaccharides of palm kernel cake as a potential prebiotic on the growth of probiotics.
30073131	0	46	from	effect	18:23	arg1	growth					104:109	the growth	100:109	the growth of probiotics	100:123	Evaluation of the effect of soluble polysaccharides of palm kernel cake as a potential prebiotic on the growth of probiotics.
30073131	6	47	from	potential	766:774	arg1	Lb					840:841	Lb	840:841	Lb	840:841	Finally, their prebiotic potential on two probiotics, namely Lactobacillus plantarum ATCC 8014 and Lb.
30073131	6	47	from	potential	766:774	arg1	ATCC					826:829	Lactobacillus plantarum ATCC 8014	802:834	Lactobacillus plantarum ATCC 8014	802:834	Finally, their prebiotic potential on two probiotics, namely Lactobacillus plantarum ATCC 8014 and Lb.
30073131	6	47	from	potential	766:774	arg1	probiotics					783:792	two probiotics	779:792	two probiotics	779:792	Finally, their prebiotic potential on two probiotics, namely Lactobacillus plantarum ATCC 8014 and Lb.
30073131	18	48	theme	novel	2472:2476	arg1	prebiotics					2478:2487	novel prebiotics	2472:2487	novel prebiotics	2472:2487	rhamnosus ATCC 53103 with decrease in the pH of the medium and producing organic acids.All the above findings strongly indicated that polysaccharides extracted from PKC, an industrial waste, have a potential to be exploited as novel prebiotics.
30073131	1	49	theme	application	211:221	arg1	examination					186:196	examination	186:196	examination	186:196	This paper deliberates the extraction, characterization and examination of potential application of soluble polysaccharides of palm kernel cake (PKC) as a prebiotic.
30073131	1	49	theme	application	211:221	arg1	characterization					165:180	characterization	165:180	characterization	165:180	This paper deliberates the extraction, characterization and examination of potential application of soluble polysaccharides of palm kernel cake (PKC) as a prebiotic.
30073131	1	49	theme	application	211:221	arg1	extraction					153:162	extraction	153:162	extraction	153:162	This paper deliberates the extraction, characterization and examination of potential application of soluble polysaccharides of palm kernel cake (PKC) as a prebiotic.
30073131	1	49	theme	application	211:221	arg1	prebiotic					281:289	a prebiotic	279:289	a prebiotic	279:289	This paper deliberates the extraction, characterization and examination of potential application of soluble polysaccharides of palm kernel cake (PKC) as a prebiotic.
30073131	1	50	theme	soluble	226:232	arg1	polysaccharides					234:248	soluble polysaccharides	226:248	soluble polysaccharides of palm kernel cake (PKC)	226:274	This paper deliberates the extraction, characterization and examination of potential application of soluble polysaccharides of palm kernel cake (PKC) as a prebiotic.
30073131	18	51	theme	producing	2308:2316	arg1	acids.All					2326:2334	the medium and producing organic acids.All	2293:2334	acids.All	2326:2334	rhamnosus ATCC 53103 with decrease in the pH of the medium and producing organic acids.All the above findings strongly indicated that polysaccharides extracted from PKC, an industrial waste, have a potential to be exploited as novel prebiotics.
30073131	17	52	theme	plantarum	2217:2225	arg1	8014					2232:2235	plantarum ATCC 8014	2217:2235	plantarum ATCC 8014	2217:2235	plantarum ATCC 8014 and Lb.
30073131	14	53	theme	SP	1955:1956	arg1	temperature					1940:1950	the main degradation temperature	1919:1950	the main degradation temperature of SP is about 121 to 125 °C	1919:1979	FTIR spectroscopy revealed that the polysaccharides extracts were linked to β and α-glycosidic bonds and thermal analysis using differential scanning calorimeter (DSC) showed the main degradation temperature of SP is about 121 to 125 °C.
30073131	13	54	theme	amide	1734:1738	arg1	1640 cm-1					1723:1731	1640 cm-1	1723:1731	1640 cm-1	1723:1731	Protein content in SCPW, SCPCA and SCPN are 0.72, 0.40 and 0.58, respectively, and the peaks which indicated the presence of protein were observed at approximately 1640 cm-1 (amide I).
30073131	13	54	theme	amide	1734:1738	arg1	I					1740:1740	amide I	1734:1740	amide I	1734:1740	Protein content in SCPW, SCPCA and SCPN are 0.72, 0.40 and 0.58, respectively, and the peaks which indicated the presence of protein were observed at approximately 1640 cm-1 (amide I).
30073131	18	55	theme	organic	2318:2324	arg1	acids.All					2326:2334	the medium and producing organic acids.All	2293:2334	acids.All	2326:2334	rhamnosus ATCC 53103 with decrease in the pH of the medium and producing organic acids.All the above findings strongly indicated that polysaccharides extracted from PKC, an industrial waste, have a potential to be exploited as novel prebiotics.
30073131	9	56	theme	crude	1092:1096	arg1	polysaccharides					1098:1112	NaOH-extracted crude polysaccharides	1077:1112	NaOH-extracted crude polysaccharides (8.73%)	1077:1120	There were significant differences (P < 0.05) between the values of NaOH-extracted crude polysaccharides (8.73%) and that of water (3.03%) and citric acid (3.07%)-extracted polysaccharides.
30073131	9	56	theme	crude	1092:1096	arg1	%					1119:1119	8.73%	1115:1119	8.73%	1115:1119	There were significant differences (P < 0.05) between the values of NaOH-extracted crude polysaccharides (8.73%) and that of water (3.03%) and citric acid (3.07%)-extracted polysaccharides.
30073131	1	57	theme	kernel	258:263	arg1	PKC					271:273	PKC	271:273	PKC	271:273	This paper deliberates the extraction, characterization and examination of potential application of soluble polysaccharides of palm kernel cake (PKC) as a prebiotic.
30073131	1	57	theme	kernel	258:263	arg1	cake					265:268	palm kernel cake	253:268	palm kernel cake (PKC)	253:274	This paper deliberates the extraction, characterization and examination of potential application of soluble polysaccharides of palm kernel cake (PKC) as a prebiotic.
30073131	14	58	theme	main	1923:1926	arg1	temperature					1940:1950	the main degradation temperature	1919:1950	the main degradation temperature of SP is about 121 to 125 °C	1919:1979	FTIR spectroscopy revealed that the polysaccharides extracts were linked to β and α-glycosidic bonds and thermal analysis using differential scanning calorimeter (DSC) showed the main degradation temperature of SP is about 121 to 125 °C.
30073131	5	59	theme	extracted	622:630	arg1	polysaccharides					640:654	The extracted soluble polysaccharides	618:654	The extracted soluble polysaccharides	618:654	The extracted soluble polysaccharides were further subjected to a digestibility test using artificial human gastric juice.
30073131	18	60	contain	have	2436:2439	arg2	potential					2443:2451	a potential to be exploited as novel prebiotics	2441:2487	a potential to be exploited as novel prebiotics	2441:2487	rhamnosus ATCC 53103 with decrease in the pH of the medium and producing organic acids.All the above findings strongly indicated that polysaccharides extracted from PKC, an industrial waste, have a potential to be exploited as novel prebiotics.
30073131	18	60	contain	have	2436:2439	arg1	polysaccharides					2379:2393	polysaccharides	2379:2393	polysaccharides extracted from PKC, an industrial waste,	2379:2434	rhamnosus ATCC 53103 with decrease in the pH of the medium and producing organic acids.All the above findings strongly indicated that polysaccharides extracted from PKC, an industrial waste, have a potential to be exploited as novel prebiotics.
30073131	6	61	theme	Lactobacillus	802:814	arg1	ATCC					826:829	Lactobacillus plantarum ATCC 8014	802:834	Lactobacillus plantarum ATCC 8014	802:834	Finally, their prebiotic potential on two probiotics, namely Lactobacillus plantarum ATCC 8014 and Lb.
30073131	6	61	theme	Lactobacillus	802:814	arg1	probiotics					783:792	two probiotics	779:792	two probiotics	779:792	Finally, their prebiotic potential on two probiotics, namely Lactobacillus plantarum ATCC 8014 and Lb.
30073131	5	62	theme	human	720:724	arg1	juice					734:738	artificial human gastric juice	709:738	artificial human gastric juice	709:738	The extracted soluble polysaccharides were further subjected to a digestibility test using artificial human gastric juice.
30073131	11	63	theme	carbohydrate	1385:1396	arg1	content					1398:1404	Total carbohydrate content	1379:1404	Total carbohydrate content in SCPW, SCPCA and SCPN	1379:1428	Total carbohydrate content in SCPW, SCPCA and SCPN are 57.11%, 56.94% and 50.95%, respectively.
30073131	11	63	theme	carbohydrate	1385:1396	arg1	%					1439:1439	57.11%	1434:1439	57.11%	1434:1439	Total carbohydrate content in SCPW, SCPCA and SCPN are 57.11%, 56.94% and 50.95%, respectively.
30073131	16	64	from	polysaccharides	2129:2143	arg1	probiotics					2148:2157	probiotics	2148:2157	probiotics	2148:2157	The prebiotics potentials of the polysaccharides on probiotics in vitro demonstrated an increase in proliferation of Lb.
30073131	0	65	theme	probiotics	114:123	arg1	growth					104:109	the growth	100:109	the growth of probiotics	100:123	Evaluation of the effect of soluble polysaccharides of palm kernel cake as a potential prebiotic on the growth of probiotics.
30073131	15	66	theme	gastric	2081:2087	arg1	juice					2089:2093	artificial human gastric juice	2064:2093	artificial human gastric juice	2064:2093	The SP were found to be highly resistance (> 96%) to hydrolysis when subjected to artificial human gastric juice.
30073131	7	67	theme	rhamnosus	844:852	arg1	ATCC					854:857	rhamnosus ATCC 53103	844:863	rhamnosus ATCC 53103	844:863	rhamnosus ATCC 53103 were evaluated in vitro.
30073131	14	68	theme	differential	1872:1883	arg1	DSC					1907:1909	DSC	1907:1909	DSC	1907:1909	FTIR spectroscopy revealed that the polysaccharides extracts were linked to β and α-glycosidic bonds and thermal analysis using differential scanning calorimeter (DSC) showed the main degradation temperature of SP is about 121 to 125 °C.
30073131	14	68	theme	differential	1872:1883	arg1	calorimeter					1894:1904	differential scanning calorimeter	1872:1904	differential scanning calorimeter (DSC)	1872:1910	FTIR spectroscopy revealed that the polysaccharides extracts were linked to β and α-glycosidic bonds and thermal analysis using differential scanning calorimeter (DSC) showed the main degradation temperature of SP is about 121 to 125 °C.
30073131	5	69	theme	digestibility	684:696	arg1	test					698:701	a digestibility test	682:701	a digestibility test using artificial human gastric juice	682:738	The extracted soluble polysaccharides were further subjected to a digestibility test using artificial human gastric juice.
30073131	15	70	theme	artificial	2064:2073	arg1	juice					2089:2093	artificial human gastric juice	2064:2093	artificial human gastric juice	2064:2093	The SP were found to be highly resistance (> 96%) to hydrolysis when subjected to artificial human gastric juice.
30073131	12	71	from	PKC	1500:1502	arg1	polysaccharides					1479:1493	The polysaccharides	1475:1493	The polysaccharides from PKC in this study	1475:1516	The polysaccharides from PKC in this study were found to be highly soluble (> 95%).
30073131	12	71	from	PKC	1500:1502	arg1	soluble					1542:1548	soluble	1542:1548	soluble	1542:1548	The polysaccharides from PKC in this study were found to be highly soluble (> 95%).
30073131	12	71	from	PKC	1500:1502	arg1	%					1555:1555	> 95%	1551:1555	> 95%	1551:1555	The polysaccharides from PKC in this study were found to be highly soluble (> 95%).
30073131	11	72	from	content	1398:1404	arg1	SCPCA					1415:1419	SCPCA	1415:1419	SCPCA	1415:1419	Total carbohydrate content in SCPW, SCPCA and SCPN are 57.11%, 56.94% and 50.95%, respectively.
30073131	11	72	from	content	1398:1404	arg1	SCPN					1425:1428	SCPN	1425:1428	SCPN	1425:1428	Total carbohydrate content in SCPW, SCPCA and SCPN are 57.11%, 56.94% and 50.95%, respectively.
30073131	14	73	theme	FTIR	1744:1747	arg1	spectroscopy					1749:1760	FTIR spectroscopy	1744:1760	FTIR spectroscopy	1744:1760	FTIR spectroscopy revealed that the polysaccharides extracts were linked to β and α-glycosidic bonds and thermal analysis using differential scanning calorimeter (DSC) showed the main degradation temperature of SP is about 121 to 125 °C.
30073131	0	74	theme	kernel	60:65	arg1	polysaccharides					36:50	soluble polysaccharides	28:50	soluble polysaccharides of palm kernel cake as a potential prebiotic	28:95	Evaluation of the effect of soluble polysaccharides of palm kernel cake as a potential prebiotic on the growth of probiotics.
30073131	4	75	theme	protein	487:493	arg1	carbohydrates					472:484	total carbohydrates	466:484	total carbohydrates	466:484	total carbohydrates, protein content, solubility rate, monosaccharides composition, structural information and thermal properties were also determined.
30073131	4	75	theme	protein	487:493	arg1	content					495:501	protein content	487:501	protein content	487:501	total carbohydrates, protein content, solubility rate, monosaccharides composition, structural information and thermal properties were also determined.
30073131	14	76	theme	thermal	1849:1855	arg1	analysis					1857:1864	thermal analysis	1849:1864	thermal analysis using differential scanning calorimeter (DSC)	1849:1910	FTIR spectroscopy revealed that the polysaccharides extracts were linked to β and α-glycosidic bonds and thermal analysis using differential scanning calorimeter (DSC) showed the main degradation temperature of SP is about 121 to 125 °C.
30073131	12	77	dep	soluble	1542:1548	arg1	polysaccharides					1479:1493	The polysaccharides	1475:1493	The polysaccharides from PKC in this study	1475:1516	The polysaccharides from PKC in this study were found to be highly soluble (> 95%).
30073131	12	77	dep	soluble	1542:1548	arg1	soluble					1542:1548	soluble	1542:1548	soluble	1542:1548	The polysaccharides from PKC in this study were found to be highly soluble (> 95%).
30073131	12	77	dep	soluble	1542:1548	arg1	%					1555:1555	> 95%	1551:1555	> 95%	1551:1555	The polysaccharides from PKC in this study were found to be highly soluble (> 95%).
30073131	0	78	theme	potential	77:85	arg1	prebiotic					87:95	a potential prebiotic	75:95	a potential prebiotic	75:95	Evaluation of the effect of soluble polysaccharides of palm kernel cake as a potential prebiotic on the growth of probiotics.
30073131	3	79	dep	viz	461:463	arg1	properties					417:426	The physiochemical properties	398:426	The physiochemical properties of the extracted polysaccharides	398:459	The physiochemical properties of the extracted polysaccharides viz.
30073131	10	80	theme	mannose	1330:1336	arg1	percentage					1316:1325	highest percentage	1308:1325	highest percentage of mannose (62.49%) and galactose (25.42%) in SPCA	1308:1376	The extracted polysaccharides composed of mannose, galactose, glucose, arabinose, xylose and rhamanose, with highest percentage of mannose (62.49%) and galactose (25.42%) in SPCA.
30073131	18	81	from	decrease	2271:2278	arg1	pH					2287:2288	the pH	2283:2288	the pH of the medium and producing organic acids.All	2283:2334	rhamnosus ATCC 53103 with decrease in the pH of the medium and producing organic acids.All the above findings strongly indicated that polysaccharides extracted from PKC, an industrial waste, have a potential to be exploited as novel prebiotics.
30073131	18	82	dep	ATCC	2255:2258	arg1	decrease					2271:2278	decrease	2271:2278	decrease in the pH of the medium and producing organic acids.All	2271:2334	rhamnosus ATCC 53103 with decrease in the pH of the medium and producing organic acids.All the above findings strongly indicated that polysaccharides extracted from PKC, an industrial waste, have a potential to be exploited as novel prebiotics.
30073131	0	83	theme	effect	18:23	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of the effect of soluble polysaccharides of palm kernel cake as a potential prebiotic on the growth of probiotics.	0:124	Evaluation of the effect of soluble polysaccharides of palm kernel cake as a potential prebiotic on the growth of probiotics.
30073131	3	84	theme	polysaccharides	445:459	arg1	properties					417:426	The physiochemical properties	398:426	The physiochemical properties of the extracted polysaccharides	398:459	The physiochemical properties of the extracted polysaccharides viz.
30073131	0	85	theme	soluble	28:34	arg1	polysaccharides					36:50	soluble polysaccharides	28:50	soluble polysaccharides of palm kernel cake as a potential prebiotic	28:95	Evaluation of the effect of soluble polysaccharides of palm kernel cake as a potential prebiotic on the growth of probiotics.
30073131	12	86	from	polysaccharides	1479:1493	arg1	study					1512:1516	this study	1507:1516	this study	1507:1516	The polysaccharides from PKC in this study were found to be highly soluble (> 95%).
30073131	3	87	theme	physiochemical	402:415	arg1	properties					417:426	The physiochemical properties	398:426	The physiochemical properties of the extracted polysaccharides	398:459	The physiochemical properties of the extracted polysaccharides viz.
30073131	14	88	theme	polysaccharides	1780:1794	arg1	extracts					1796:1803	the polysaccharides extracts	1776:1803	the polysaccharides extracts	1776:1803	FTIR spectroscopy revealed that the polysaccharides extracts were linked to β and α-glycosidic bonds and thermal analysis using differential scanning calorimeter (DSC) showed the main degradation temperature of SP is about 121 to 125 °C.
30073131	16	89	theme	Lb	2213:2214	arg1	proliferation					2196:2208	proliferation	2196:2208	proliferation of Lb	2196:2214	The prebiotics potentials of the polysaccharides on probiotics in vitro demonstrated an increase in proliferation of Lb.
30073131	4	90	theme	monosaccharides	521:535	arg1	carbohydrates					472:484	total carbohydrates	466:484	total carbohydrates	466:484	total carbohydrates, protein content, solubility rate, monosaccharides composition, structural information and thermal properties were also determined.
30073131	4	90	theme	monosaccharides	521:535	arg1	composition					537:547	monosaccharides composition	521:547	monosaccharides composition	521:547	total carbohydrates, protein content, solubility rate, monosaccharides composition, structural information and thermal properties were also determined.
30073131	16	91	theme	prebiotics	2100:2109	arg1	potentials					2111:2120	The prebiotics potentials	2096:2120	The prebiotics potentials of the polysaccharides on probiotics	2096:2157	The prebiotics potentials of the polysaccharides on probiotics in vitro demonstrated an increase in proliferation of Lb.
30073131	9	92	dep	water	1134:1138	arg1	polysaccharides					1182:1196	-extracted polysaccharides	1171:1196	-extracted polysaccharides	1171:1196	There were significant differences (P < 0.05) between the values of NaOH-extracted crude polysaccharides (8.73%) and that of water (3.03%) and citric acid (3.07%)-extracted polysaccharides.
30073131	10	93	theme	galactose	1351:1359	arg1	percentage					1316:1325	highest percentage	1308:1325	highest percentage of mannose (62.49%) and galactose (25.42%) in SPCA	1308:1376	The extracted polysaccharides composed of mannose, galactose, glucose, arabinose, xylose and rhamanose, with highest percentage of mannose (62.49%) and galactose (25.42%) in SPCA.
30073131	6	94	theme	prebiotic	756:764	arg1	potential					766:774	their prebiotic potential	750:774	their prebiotic potential on two probiotics, namely Lactobacillus plantarum ATCC 8014 and Lb	750:841	Finally, their prebiotic potential on two probiotics, namely Lactobacillus plantarum ATCC 8014 and Lb.
30073131	16	95	from	potentials	2111:2120	arg1	probiotics					2148:2157	probiotics	2148:2157	probiotics	2148:2157	The prebiotics potentials of the polysaccharides on probiotics in vitro demonstrated an increase in proliferation of Lb.
30073131	14	96	attach	linked	1810:1815	arg2	extracts					1796:1803	the polysaccharides extracts	1776:1803	the polysaccharides extracts	1776:1803	FTIR spectroscopy revealed that the polysaccharides extracts were linked to β and α-glycosidic bonds and thermal analysis using differential scanning calorimeter (DSC) showed the main degradation temperature of SP is about 121 to 125 °C.
30073131	14	96	attach	linked	1810:1815	arg1	bonds					1839:1843	β and α-glycosidic bonds	1820:1843	β and α-glycosidic bonds	1820:1843	FTIR spectroscopy revealed that the polysaccharides extracts were linked to β and α-glycosidic bonds and thermal analysis using differential scanning calorimeter (DSC) showed the main degradation temperature of SP is about 121 to 125 °C.
30073131	0	97	from	growth	104:109	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of the effect of soluble polysaccharides of palm kernel cake as a potential prebiotic on the growth of probiotics.	0:124	Evaluation of the effect of soluble polysaccharides of palm kernel cake as a potential prebiotic on the growth of probiotics.
30073131	8	98	contain	contained	915:923	arg1	PKC					911:913	PKC	911:913	PKC	911:913	It was observed that PKC contained ash (5.2%), moisture (7.4%), carbohydrates (65.8%), protein (16.5%) and fat (5.1%).
30073131	8	98	contain	contained	915:923	arg2	%					973:973	65.8%	969:973	65.8%	969:973	It was observed that PKC contained ash (5.2%), moisture (7.4%), carbohydrates (65.8%), protein (16.5%) and fat (5.1%).
30073131	8	98	contain	contained	915:923	arg2	%					990:990	16.5%	986:990	16.5%	986:990	It was observed that PKC contained ash (5.2%), moisture (7.4%), carbohydrates (65.8%), protein (16.5%) and fat (5.1%).
30073131	8	98	contain	contained	915:923	arg2	%					1005:1005	5.1%	1002:1005	5.1%	1002:1005	It was observed that PKC contained ash (5.2%), moisture (7.4%), carbohydrates (65.8%), protein (16.5%) and fat (5.1%).
30073131	8	98	contain	contained	915:923	arg2	fat					997:999	fat	997:999	fat (5.1%)	997:1006	It was observed that PKC contained ash (5.2%), moisture (7.4%), carbohydrates (65.8%), protein (16.5%) and fat (5.1%).
30073131	8	98	contain	contained	915:923	arg2	%					933:933	5.2%	930:933	5.2%	930:933	It was observed that PKC contained ash (5.2%), moisture (7.4%), carbohydrates (65.8%), protein (16.5%) and fat (5.1%).
30073131	8	98	contain	contained	915:923	arg2	ash					925:927	ash	925:927	ash (5.2%)	925:934	It was observed that PKC contained ash (5.2%), moisture (7.4%), carbohydrates (65.8%), protein (16.5%) and fat (5.1%).
30073131	8	98	contain	contained	915:923	arg2	%					950:950	7.4%	947:950	7.4%	947:950	It was observed that PKC contained ash (5.2%), moisture (7.4%), carbohydrates (65.8%), protein (16.5%) and fat (5.1%).
30073131	8	98	contain	contained	915:923	arg2	moisture					937:944	moisture	937:944	moisture (7.4%)	937:951	It was observed that PKC contained ash (5.2%), moisture (7.4%), carbohydrates (65.8%), protein (16.5%) and fat (5.1%).
30073131	8	98	contain	contained	915:923	arg2	carbohydrates					954:966	carbohydrates	954:966	carbohydrates (65.8%)	954:974	It was observed that PKC contained ash (5.2%), moisture (7.4%), carbohydrates (65.8%), protein (16.5%) and fat (5.1%).
30073131	8	98	contain	contained	915:923	arg2	protein					977:983	protein	977:983	protein (16.5%)	977:991	It was observed that PKC contained ash (5.2%), moisture (7.4%), carbohydrates (65.8%), protein (16.5%) and fat (5.1%).
30073131	16	99	theme	polysaccharides	2129:2143	arg1	potentials					2111:2120	The prebiotics potentials	2096:2120	The prebiotics potentials of the polysaccharides on probiotics	2096:2157	The prebiotics potentials of the polysaccharides on probiotics in vitro demonstrated an increase in proliferation of Lb.
30073131	1	100	theme	polysaccharides	234:248	arg1	application					211:221	potential application	201:221	potential application of soluble polysaccharides of palm kernel cake (PKC)	201:274	This paper deliberates the extraction, characterization and examination of potential application of soluble polysaccharides of palm kernel cake (PKC) as a prebiotic.
29408615	8	0	from	SH-SY5Y	1153:1159	arg1	activity					1110:1117	significant activity	1098:1117	significant activity of protective effect on apoptosis SH-SY5Y induced by Aβ25-35	1098:1178	All dose groups showed significant activity of protective effect on apoptosis SH-SY5Y induced by Aβ25-35, and obviously inhibited the S phase arrest.
29408615	3	1	gly	glycoproteins	392:404	arg1	glycoproteins					392:404	The glycoproteins	388:404	The glycoproteins	388:404	The glycoproteins has a molecular weight in the range from 0.4 to 4.4kDa, with an average molecular mass of 1.6kDa.
29408615	2	2	dep	named	258:262	arg1	glycoproteins					244:256	glycoproteins	244:256	glycoproteins	244:256	A glycoproteins named PGL-1 was extracted from ginseng which subjected to through a macroporous resin, hollow-fiber ultrafiltration and dialyzed.
29408615	7	3	theme	rats	1050:1053	arg1	symptoms					1038:1045	the memory impairment symptoms	1016:1045	the memory impairment symptoms of rats induced by Aβ25-35	1016:1072	The Morris water maze test revealed that the PGL-1 can effectively alleviate the memory impairment symptoms of rats induced by Aβ25-35.
29408615	8	4	theme	apoptosis	1143:1151	arg1	SH-SY5Y					1153:1159	apoptosis SH-SY5Y	1143:1159	apoptosis SH-SY5Y induced by Aβ25-35	1143:1178	All dose groups showed significant activity of protective effect on apoptosis SH-SY5Y induced by Aβ25-35, and obviously inhibited the S phase arrest.
29408615	0	5	theme	glycoproteins	62:74	arg1	effects					37:43	In vivo and in vitro neuroprotective effects	0:43	In vivo and in vitro neuroprotective effects of Panax ginseng glycoproteins	0:74	In vivo and in vitro neuroprotective effects of Panax ginseng glycoproteins.
29408615	8	6	theme	phase	1211:1215	arg1	arrest					1217:1222	the S phase arrest	1205:1222	the S phase arrest	1205:1222	All dose groups showed significant activity of protective effect on apoptosis SH-SY5Y induced by Aβ25-35, and obviously inhibited the S phase arrest.
29408615	7	7	theme	Morris	943:948	arg1	test					961:964	The Morris water maze test	939:964	The Morris water maze test	939:964	The Morris water maze test revealed that the PGL-1 can effectively alleviate the memory impairment symptoms of rats induced by Aβ25-35.
29408615	8	8	theme	S	1209:1209	arg1	arrest					1217:1222	the S phase arrest	1205:1222	the S phase arrest	1205:1222	All dose groups showed significant activity of protective effect on apoptosis SH-SY5Y induced by Aβ25-35, and obviously inhibited the S phase arrest.
29408615	2	9	gly	glycoproteins	244:256	arg1	glycoproteins					244:256	glycoproteins	244:256	glycoproteins	244:256	A glycoproteins named PGL-1 was extracted from ginseng which subjected to through a macroporous resin, hollow-fiber ultrafiltration and dialyzed.
29408615	7	10	theme	impairment	1027:1036	arg1	symptoms					1038:1045	the memory impairment symptoms	1016:1045	the memory impairment symptoms of rats induced by Aβ25-35	1016:1072	The Morris water maze test revealed that the PGL-1 can effectively alleviate the memory impairment symptoms of rats induced by Aβ25-35.
29408615	8	11	theme	protective	1122:1131	arg1	effect					1133:1138	protective effect	1122:1138	protective effect on apoptosis SH-SY5Y induced by Aβ25-35	1122:1178	All dose groups showed significant activity of protective effect on apoptosis SH-SY5Y induced by Aβ25-35, and obviously inhibited the S phase arrest.
29408615	2	12	theme	named	258:262	arg1	PGL-1					264:268	A glycoproteins named PGL-1	242:268	A glycoproteins named PGL-1	242:268	A glycoproteins named PGL-1 was extracted from ginseng which subjected to through a macroporous resin, hollow-fiber ultrafiltration and dialyzed.
29408615	4	13	theme	galacturonic	611:622	arg1	acid					624:627	galacturonic acid	611:627	galacturonic acid	611:627	HPLC analysis revealed that the compositions of glycoproteins included fucose, mannose, rhamnose, glucose, galacturonic acid, N-acetylglucosamine and N-acetylgalactosamine.
29408615	1	14	theme	cognitive	222:230	arg1	function					232:239	cognitive function	222:239	cognitive function	222:239	The root of Panax ginseng C. A. Mey (Araliaceae) has medicinal value in complex system of Traditional Chinese medicines for its use in improving cognitive function.
29408615	1	15	contain	has	126:128	arg1	root					81:84	The root	77:84	The root of Panax ginseng C. A. Mey (Araliaceae)	77:124	The root of Panax ginseng C. A. Mey (Araliaceae) has medicinal value in complex system of Traditional Chinese medicines for its use in improving cognitive function.
29408615	1	15	contain	has	126:128	arg2	value					140:144	medicinal value	130:144	medicinal value	130:144	The root of Panax ginseng C. A. Mey (Araliaceae) has medicinal value in complex system of Traditional Chinese medicines for its use in improving cognitive function.
29408615	8	16	from	activity	1110:1117	arg1	SH-SY5Y					1153:1159	apoptosis SH-SY5Y	1143:1159	apoptosis SH-SY5Y induced by Aβ25-35	1143:1178	All dose groups showed significant activity of protective effect on apoptosis SH-SY5Y induced by Aβ25-35, and obviously inhibited the S phase arrest.
29408615	7	17	theme	water	950:954	arg1	test					961:964	The Morris water maze test	939:964	The Morris water maze test	939:964	The Morris water maze test revealed that the PGL-1 can effectively alleviate the memory impairment symptoms of rats induced by Aβ25-35.
29408615	10	18	gly	glycoproteins	1389:1401	arg1	reagent					1453:1459	a promising anti-AD reagent	1433:1459	a promising anti-AD reagent	1433:1459	Thus, glycoproteins derived from ginseng might be a promising anti-AD reagent.
29408615	10	18	gly	glycoproteins	1389:1401	arg1	glycoproteins					1389:1401	glycoproteins	1389:1401	glycoproteins derived from ginseng	1389:1422	Thus, glycoproteins derived from ginseng might be a promising anti-AD reagent.
29408615	6	19	theme	1→	853:854	arg1	-Glc					866:869	(1→)-Glc	862:869	(1→)-Glc	862:869	The (1→)-Fuc, (1→)-Glc and (1→)-GlcNAc or (1→)-GalNAc were regarded as a terminal residue.
29408615	6	19	theme	1→	853:854	arg1	-GlcNAc					879:885	-GlcNAc	879:885	-GlcNAc	879:885	The (1→)-Fuc, (1→)-Glc and (1→)-GlcNAc or (1→)-GalNAc were regarded as a terminal residue.
29408615	6	19	theme	1→	853:854	arg1	residue					930:936	a terminal residue	919:936	a terminal residue	919:936	The (1→)-Fuc, (1→)-Glc and (1→)-GlcNAc or (1→)-GalNAc were regarded as a terminal residue.
29408615	6	19	theme	1→	853:854	arg1	-Fuc					856:859	The (1→)-Fuc	848:859	The (1→)-Fuc	848:859	The (1→)-Fuc, (1→)-Glc and (1→)-GlcNAc or (1→)-GalNAc were regarded as a terminal residue.
29408615	0	20	gly	glycoproteins	62:74	arg1	glycoproteins					62:74	Panax ginseng glycoproteins	48:74	Panax ginseng glycoproteins	48:74	In vivo and in vitro neuroprotective effects of Panax ginseng glycoproteins.
29408615	1	21	theme	medicinal	130:138	arg1	value					140:144	medicinal value	130:144	medicinal value	130:144	The root of Panax ginseng C. A. Mey (Araliaceae) has medicinal value in complex system of Traditional Chinese medicines for its use in improving cognitive function.
29408615	9	22	located	detected	1329:1336	arg1	cells					1341:1345	cells	1341:1345	cells co-administered with glycoproteins	1341:1380	Compared with Aβ25-35 treatment alone, a significant reduction in NO concentration and NOS activity was detected in cells co-administered with glycoproteins.
29408615	9	22	located	detected	1329:1336	arg2	reduction					1278:1286	a significant reduction	1264:1286	a significant reduction in NO concentration and NOS activity	1264:1323	Compared with Aβ25-35 treatment alone, a significant reduction in NO concentration and NOS activity was detected in cells co-administered with glycoproteins.
29408615	5	23	dep	-Fuc	727:730	arg1	1→					796:797	1→	796:797	1→	796:797	Glycan of PGL-1 has a backbone of →4)-Rha-(1→, →4)-Fuc -(1→, →6)-Gal-(1→, →4)-GalA-(1→, →4)-GlcNAc-(1→ and →4)-GalNAc-(1→,and (→3,6)-Man-(1→) was distributed in branches.
29408615	5	23	dep	-Fuc	727:730	arg1	→4					724:725	→4)-Rha-(1→, →4)-Fuc	711:730	→4	724:725	Glycan of PGL-1 has a backbone of →4)-Rha-(1→, →4)-Fuc -(1→, →6)-Gal-(1→, →4)-GalA-(1→, →4)-GlcNAc-(1→ and →4)-GalNAc-(1→,and (→3,6)-Man-(1→) was distributed in branches.
29408615	9	24	theme	NOS	1312:1314	arg1	activity					1316:1323	NOS activity	1312:1323	NOS activity	1312:1323	Compared with Aβ25-35 treatment alone, a significant reduction in NO concentration and NOS activity was detected in cells co-administered with glycoproteins.
29408615	5	25	theme	1→	720:721	arg1	-Fuc					727:730	→4)-Rha-(1→, →4)-Fuc	711:730	-Fuc	727:730	Glycan of PGL-1 has a backbone of →4)-Rha-(1→, →4)-Fuc -(1→, →6)-Gal-(1→, →4)-GalA-(1→, →4)-GlcNAc-(1→ and →4)-GalNAc-(1→,and (→3,6)-Man-(1→) was distributed in branches.
29408615	7	26	theme	maze	956:959	arg1	test					961:964	The Morris water maze test	939:964	The Morris water maze test	939:964	The Morris water maze test revealed that the PGL-1 can effectively alleviate the memory impairment symptoms of rats induced by Aβ25-35.
29408615	6	27	dep	-Glc	866:869	arg1	1→					863:864	1→	863:864	1→	863:864	The (1→)-Fuc, (1→)-Glc and (1→)-GlcNAc or (1→)-GalNAc were regarded as a terminal residue.
29408615	6	28	dep	-GlcNAc	879:885	arg1	1→					876:877	1→	876:877	1→	876:877	The (1→)-Fuc, (1→)-Glc and (1→)-GlcNAc or (1→)-GalNAc were regarded as a terminal residue.
29408615	0	29	theme	In	0:1	arg1	effects					37:43	In vivo and in vitro neuroprotective effects	0:43	In vivo and in vitro neuroprotective effects of Panax ginseng glycoproteins	0:74	In vivo and in vitro neuroprotective effects of Panax ginseng glycoproteins.
29408615	5	30	contain	has	693:695	arg2	backbone					699:706	a backbone	697:706	a backbone of →4)-Rha-(1→, →4)-Fuc -(1→, →6)-Gal-(1→, →4)-GalA-(1→, →4)-GlcNAc-(1→ and →4)-GalNAc-(1→	697:797	Glycan of PGL-1 has a backbone of →4)-Rha-(1→, →4)-Fuc -(1→, →6)-Gal-(1→, →4)-GalA-(1→, →4)-GlcNAc-(1→ and →4)-GalNAc-(1→,and (→3,6)-Man-(1→) was distributed in branches.
29408615	5	30	contain	has	693:695	arg1	Glycan					677:682	Glycan	677:682	Glycan of PGL-1	677:691	Glycan of PGL-1 has a backbone of →4)-Rha-(1→, →4)-Fuc -(1→, →6)-Gal-(1→, →4)-GalA-(1→, →4)-GlcNAc-(1→ and →4)-GalNAc-(1→,and (→3,6)-Man-(1→) was distributed in branches.
29408615	3	31	from	4.4kDa	454:459	arg1	range					436:440	the range	432:440	the range from 0.4 to 4.4kDa	432:459	The glycoproteins has a molecular weight in the range from 0.4 to 4.4kDa, with an average molecular mass of 1.6kDa.
29408615	2	32	dep	ginseng	289:295	arg1	subjected					303:311	subjected	303:311	subjected to	303:314	A glycoproteins named PGL-1 was extracted from ginseng which subjected to through a macroporous resin, hollow-fiber ultrafiltration and dialyzed.
29408615	2	32	dep	ginseng	289:295	arg1	which					297:301	which	297:301	which	297:301	A glycoproteins named PGL-1 was extracted from ginseng which subjected to through a macroporous resin, hollow-fiber ultrafiltration and dialyzed.
29408615	1	33	theme	complex	149:155	arg1	system					157:162	complex system	149:162	complex system of Traditional Chinese medicines for its use in improving cognitive function	149:239	The root of Panax ginseng C. A. Mey (Araliaceae) has medicinal value in complex system of Traditional Chinese medicines for its use in improving cognitive function.
29408615	0	34	theme	in	12:13	arg1	effects					37:43	In vivo and in vitro neuroprotective effects	0:43	In vivo and in vitro neuroprotective effects of Panax ginseng glycoproteins	0:74	In vivo and in vitro neuroprotective effects of Panax ginseng glycoproteins.
29408615	4	35	theme	HPLC	504:507	arg1	analysis					509:516	HPLC analysis	504:516	HPLC analysis	504:516	HPLC analysis revealed that the compositions of glycoproteins included fucose, mannose, rhamnose, glucose, galacturonic acid, N-acetylglucosamine and N-acetylgalactosamine.
29408615	10	36	theme	promising	1435:1443	arg1	reagent					1453:1459	a promising anti-AD reagent	1433:1459	a promising anti-AD reagent	1433:1459	Thus, glycoproteins derived from ginseng might be a promising anti-AD reagent.
29408615	10	36	theme	promising	1435:1443	arg1	glycoproteins					1389:1401	glycoproteins	1389:1401	glycoproteins derived from ginseng	1389:1422	Thus, glycoproteins derived from ginseng might be a promising anti-AD reagent.
29408615	4	37	gly	glycoproteins	552:564	arg1	glycoproteins					552:564	glycoproteins	552:564	glycoproteins	552:564	HPLC analysis revealed that the compositions of glycoproteins included fucose, mannose, rhamnose, glucose, galacturonic acid, N-acetylglucosamine and N-acetylgalactosamine.
29408615	8	38	theme	dose	1079:1082	arg1	groups					1084:1089	All dose groups	1075:1089	All dose groups	1075:1089	All dose groups showed significant activity of protective effect on apoptosis SH-SY5Y induced by Aβ25-35, and obviously inhibited the S phase arrest.
29408615	8	39	from	effect	1133:1138	arg1	SH-SY5Y					1153:1159	apoptosis SH-SY5Y	1143:1159	apoptosis SH-SY5Y induced by Aβ25-35	1143:1178	All dose groups showed significant activity of protective effect on apoptosis SH-SY5Y induced by Aβ25-35, and obviously inhibited the S phase arrest.
29408615	5	40	theme	-GalNAc-	787:794	arg1	backbone					699:706	a backbone	697:706	a backbone of →4)-Rha-(1→, →4)-Fuc -(1→, →6)-Gal-(1→, →4)-GalA-(1→, →4)-GlcNAc-(1→ and →4)-GalNAc-(1→	697:797	Glycan of PGL-1 has a backbone of →4)-Rha-(1→, →4)-Fuc -(1→, →6)-Gal-(1→, →4)-GalA-(1→, →4)-GlcNAc-(1→ and →4)-GalNAc-(1→,and (→3,6)-Man-(1→) was distributed in branches.
29408615	9	41	theme	significant	1266:1276	arg1	reduction					1278:1286	a significant reduction	1264:1286	a significant reduction in NO concentration and NOS activity	1264:1323	Compared with Aβ25-35 treatment alone, a significant reduction in NO concentration and NOS activity was detected in cells co-administered with glycoproteins.
29408615	3	42	dep	4.4kDa	454:459	arg1	to					451:452	to	451:452	to	451:452	The glycoproteins has a molecular weight in the range from 0.4 to 4.4kDa, with an average molecular mass of 1.6kDa.
29408615	1	43	theme	Panax	89:93	arg1	root					81:84	The root	77:84	The root of Panax ginseng C. A. Mey (Araliaceae)	77:124	The root of Panax ginseng C. A. Mey (Araliaceae) has medicinal value in complex system of Traditional Chinese medicines for its use in improving cognitive function.
29408615	3	44	theme	average	470:476	arg1	mass					488:491	an average molecular mass	467:491	an average molecular mass of 1.6kDa	467:501	The glycoproteins has a molecular weight in the range from 0.4 to 4.4kDa, with an average molecular mass of 1.6kDa.
29408615	0	45	theme	neuroprotective	21:35	arg1	effects					37:43	In vivo and in vitro neuroprotective effects	0:43	In vivo and in vitro neuroprotective effects of Panax ginseng glycoproteins	0:74	In vivo and in vitro neuroprotective effects of Panax ginseng glycoproteins.
29408615	5	46	theme	PGL-1	687:691	arg1	Glycan					677:682	Glycan	677:682	Glycan of PGL-1	677:691	Glycan of PGL-1 has a backbone of →4)-Rha-(1→, →4)-Fuc -(1→, →6)-Gal-(1→, →4)-GalA-(1→, →4)-GlcNAc-(1→ and →4)-GalNAc-(1→,and (→3,6)-Man-(1→) was distributed in branches.
29408615	5	47	theme	-Gal-	741:745	arg1	-GalNAc-					787:794	(1→, →6)-Gal-(1→, →4)-GalA-(1→, →4)-GlcNAc-(1→ and →4)-GalNAc-	733:794	-GalNAc-	787:794	Glycan of PGL-1 has a backbone of →4)-Rha-(1→, →4)-Fuc -(1→, →6)-Gal-(1→, →4)-GalA-(1→, →4)-GlcNAc-(1→ and →4)-GalNAc-(1→,and (→3,6)-Man-(1→) was distributed in branches.
29408615	9	48	theme	NO	1291:1292	arg1	concentration					1294:1306	NO concentration	1291:1306	NO concentration	1291:1306	Compared with Aβ25-35 treatment alone, a significant reduction in NO concentration and NOS activity was detected in cells co-administered with glycoproteins.
29408615	7	49	theme	memory	1020:1025	arg1	symptoms					1038:1045	the memory impairment symptoms	1016:1045	the memory impairment symptoms of rats induced by Aβ25-35	1016:1072	The Morris water maze test revealed that the PGL-1 can effectively alleviate the memory impairment symptoms of rats induced by Aβ25-35.
29408615	1	50	theme	Traditional	167:177	arg1	medicines					187:195	Traditional Chinese medicines	167:195	Traditional Chinese medicines	167:195	The root of Panax ginseng C. A. Mey (Araliaceae) has medicinal value in complex system of Traditional Chinese medicines for its use in improving cognitive function.
29408615	3	51	theme	molecular	412:420	arg1	weight					422:427	a molecular weight	410:427	a molecular weight	410:427	The glycoproteins has a molecular weight in the range from 0.4 to 4.4kDa, with an average molecular mass of 1.6kDa.
29408615	1	52	theme	Chinese	179:185	arg1	medicines					187:195	Traditional Chinese medicines	167:195	Traditional Chinese medicines	167:195	The root of Panax ginseng C. A. Mey (Araliaceae) has medicinal value in complex system of Traditional Chinese medicines for its use in improving cognitive function.
29408615	5	53	theme	-GalA-	754:759	arg1	-GalNAc-					787:794	(1→, →6)-Gal-(1→, →4)-GalA-(1→, →4)-GlcNAc-(1→ and →4)-GalNAc-	733:794	-GalNAc-	787:794	Glycan of PGL-1 has a backbone of →4)-Rha-(1→, →4)-Fuc -(1→, →6)-Gal-(1→, →4)-GalA-(1→, →4)-GlcNAc-(1→ and →4)-GalNAc-(1→,and (→3,6)-Man-(1→) was distributed in branches.
29408615	5	54	theme	-Rha-	714:718	arg1	-Fuc					727:730	→4)-Rha-(1→, →4)-Fuc	711:730	-Fuc	727:730	Glycan of PGL-1 has a backbone of →4)-Rha-(1→, →4)-Fuc -(1→, →6)-Gal-(1→, →4)-GalA-(1→, →4)-GlcNAc-(1→ and →4)-GalNAc-(1→,and (→3,6)-Man-(1→) was distributed in branches.
29408615	6	55	theme	terminal	921:928	arg1	-Glc					866:869	(1→)-Glc	862:869	(1→)-Glc	862:869	The (1→)-Fuc, (1→)-Glc and (1→)-GlcNAc or (1→)-GalNAc were regarded as a terminal residue.
29408615	6	55	theme	terminal	921:928	arg1	-GlcNAc					879:885	-GlcNAc	879:885	-GlcNAc	879:885	The (1→)-Fuc, (1→)-Glc and (1→)-GlcNAc or (1→)-GalNAc were regarded as a terminal residue.
29408615	6	55	theme	terminal	921:928	arg1	residue					930:936	a terminal residue	919:936	a terminal residue	919:936	The (1→)-Fuc, (1→)-Glc and (1→)-GlcNAc or (1→)-GalNAc were regarded as a terminal residue.
29408615	6	55	theme	terminal	921:928	arg1	-Fuc					856:859	The (1→)-Fuc	848:859	The (1→)-Fuc	848:859	The (1→)-Fuc, (1→)-Glc and (1→)-GlcNAc or (1→)-GalNAc were regarded as a terminal residue.
29408615	5	56	theme	-Fuc	727:730	arg1	backbone					699:706	a backbone	697:706	a backbone of →4)-Rha-(1→, →4)-Fuc -(1→, →6)-Gal-(1→, →4)-GalA-(1→, →4)-GlcNAc-(1→ and →4)-GalNAc-(1→	697:797	Glycan of PGL-1 has a backbone of →4)-Rha-(1→, →4)-Fuc -(1→, →6)-Gal-(1→, →4)-GalA-(1→, →4)-GlcNAc-(1→ and →4)-GalNAc-(1→,and (→3,6)-Man-(1→) was distributed in branches.
29408615	4	57	theme	glycoproteins	552:564	arg1	compositions					536:547	the compositions	532:547	the compositions of glycoproteins	532:564	HPLC analysis revealed that the compositions of glycoproteins included fucose, mannose, rhamnose, glucose, galacturonic acid, N-acetylglucosamine and N-acetylgalactosamine.
29408615	8	58	theme	effect	1133:1138	arg1	activity					1110:1117	significant activity	1098:1117	significant activity of protective effect on apoptosis SH-SY5Y induced by Aβ25-35	1098:1178	All dose groups showed significant activity of protective effect on apoptosis SH-SY5Y induced by Aβ25-35, and obviously inhibited the S phase arrest.
29408615	6	59	dep	-GalNAc	894:900	arg1	1→					891:892	1→	891:892	1→	891:892	The (1→)-Fuc, (1→)-Glc and (1→)-GlcNAc or (1→)-GalNAc were regarded as a terminal residue.
29408615	9	60	from	reduction	1278:1286	arg1	concentration					1294:1306	NO concentration	1291:1306	NO concentration	1291:1306	Compared with Aβ25-35 treatment alone, a significant reduction in NO concentration and NOS activity was detected in cells co-administered with glycoproteins.
29408615	9	60	from	reduction	1278:1286	arg1	activity					1316:1323	NOS activity	1312:1323	NOS activity	1312:1323	Compared with Aβ25-35 treatment alone, a significant reduction in NO concentration and NOS activity was detected in cells co-administered with glycoproteins.
29408615	2	61	dep	ultrafiltration	358:372	arg1	hollow-fiber					345:356	a macroporous resin, hollow-fiber ultrafiltration	324:372	hollow-fiber	345:356	A glycoproteins named PGL-1 was extracted from ginseng which subjected to through a macroporous resin, hollow-fiber ultrafiltration and dialyzed.
29408615	10	62	attach	derived	1403:1409	arg2	glycoproteins					1389:1401	glycoproteins	1389:1401	glycoproteins derived from ginseng	1389:1422	Thus, glycoproteins derived from ginseng might be a promising anti-AD reagent.
29408615	10	62	attach	derived	1403:1409	arg1	ginseng					1416:1422	ginseng	1416:1422	ginseng	1416:1422	Thus, glycoproteins derived from ginseng might be a promising anti-AD reagent.
29408615	10	62	attach	derived	1403:1409	arg2	reagent					1453:1459	a promising anti-AD reagent	1433:1459	a promising anti-AD reagent	1433:1459	Thus, glycoproteins derived from ginseng might be a promising anti-AD reagent.
29408615	3	63	contain	has	406:408	arg2	weight					422:427	a molecular weight	410:427	a molecular weight	410:427	The glycoproteins has a molecular weight in the range from 0.4 to 4.4kDa, with an average molecular mass of 1.6kDa.
29408615	3	63	contain	has	406:408	arg1	glycoproteins					392:404	The glycoproteins	388:404	The glycoproteins	388:404	The glycoproteins has a molecular weight in the range from 0.4 to 4.4kDa, with an average molecular mass of 1.6kDa.
29408615	0	64	dep	in	12:13	arg1	vitro					15:19	vitro	15:19	vitro	15:19	In vivo and in vitro neuroprotective effects of Panax ginseng glycoproteins.
29408615	5	65	theme	-GlcNAc-	768:775	arg1	-GalNAc-					787:794	(1→, →6)-Gal-(1→, →4)-GalA-(1→, →4)-GlcNAc-(1→ and →4)-GalNAc-	733:794	-GalNAc-	787:794	Glycan of PGL-1 has a backbone of →4)-Rha-(1→, →4)-Fuc -(1→, →6)-Gal-(1→, →4)-GalA-(1→, →4)-GlcNAc-(1→ and →4)-GalNAc-(1→,and (→3,6)-Man-(1→) was distributed in branches.
29408615	10	66	theme	anti-AD	1445:1451	arg1	reagent					1453:1459	a promising anti-AD reagent	1433:1459	a promising anti-AD reagent	1433:1459	Thus, glycoproteins derived from ginseng might be a promising anti-AD reagent.
29408615	10	66	theme	anti-AD	1445:1451	arg1	glycoproteins					1389:1401	glycoproteins	1389:1401	glycoproteins derived from ginseng	1389:1422	Thus, glycoproteins derived from ginseng might be a promising anti-AD reagent.
29408615	1	67	theme	medicines	187:195	arg1	system					157:162	complex system	149:162	complex system of Traditional Chinese medicines for its use in improving cognitive function	149:239	The root of Panax ginseng C. A. Mey (Araliaceae) has medicinal value in complex system of Traditional Chinese medicines for its use in improving cognitive function.
29408615	9	68	theme	Aβ25-35	1239:1245	arg1	treatment					1247:1255	Aβ25-35 treatment	1239:1255	Aβ25-35 treatment alone	1239:1261	Compared with Aβ25-35 treatment alone, a significant reduction in NO concentration and NOS activity was detected in cells co-administered with glycoproteins.
29408615	3	69	theme	1.6kDa	496:501	arg1	mass					488:491	an average molecular mass	467:491	an average molecular mass of 1.6kDa	467:501	The glycoproteins has a molecular weight in the range from 0.4 to 4.4kDa, with an average molecular mass of 1.6kDa.
29408615	3	70	theme	molecular	478:486	arg1	mass					488:491	an average molecular mass	467:491	an average molecular mass of 1.6kDa	467:501	The glycoproteins has a molecular weight in the range from 0.4 to 4.4kDa, with an average molecular mass of 1.6kDa.
29408615	9	71	gly	glycoproteins	1368:1380	arg1	glycoproteins					1368:1380	glycoproteins	1368:1380	glycoproteins	1368:1380	Compared with Aβ25-35 treatment alone, a significant reduction in NO concentration and NOS activity was detected in cells co-administered with glycoproteins.
29408615	5	72	dep	-GalNAc-	787:794	arg1	→4					784:785	→4	784:785	→4	784:785	Glycan of PGL-1 has a backbone of →4)-Rha-(1→, →4)-Fuc -(1→, →6)-Gal-(1→, →4)-GalA-(1→, →4)-GlcNAc-(1→ and →4)-GalNAc-(1→,and (→3,6)-Man-(1→) was distributed in branches.
29408615	5	72	dep	-GalNAc-	787:794	arg1	1→					777:778	1→	777:778	1→	777:778	Glycan of PGL-1 has a backbone of →4)-Rha-(1→, →4)-Fuc -(1→, →6)-Gal-(1→, →4)-GalA-(1→, →4)-GlcNAc-(1→ and →4)-GalNAc-(1→,and (→3,6)-Man-(1→) was distributed in branches.
29408615	0	73	theme	ginseng	54:60	arg1	glycoproteins					62:74	Panax ginseng glycoproteins	48:74	Panax ginseng glycoproteins	48:74	In vivo and in vitro neuroprotective effects of Panax ginseng glycoproteins.
29408615	5	74	theme	→3,6	804:807	arg1	1→					815:816	1→	815:816	1→	815:816	Glycan of PGL-1 has a backbone of →4)-Rha-(1→, →4)-Fuc -(1→, →6)-Gal-(1→, →4)-GalA-(1→, →4)-GlcNAc-(1→ and →4)-GalNAc-(1→,and (→3,6)-Man-(1→) was distributed in branches.
29408615	5	74	theme	→3,6	804:807	arg1	-Man-					809:813	(→3,6)-Man-	803:813	(→3,6)-Man-(1→)	803:817	Glycan of PGL-1 has a backbone of →4)-Rha-(1→, →4)-Fuc -(1→, →6)-Gal-(1→, →4)-GalA-(1→, →4)-GlcNAc-(1→ and →4)-GalNAc-(1→,and (→3,6)-Man-(1→) was distributed in branches.
29408615	2	75	theme	resin	338:342	arg1	ultrafiltration					358:372	a macroporous resin, hollow-fiber ultrafiltration	324:372	ultrafiltration	358:372	A glycoproteins named PGL-1 was extracted from ginseng which subjected to through a macroporous resin, hollow-fiber ultrafiltration and dialyzed.
29408615	0	76	theme	Panax	48:52	arg1	glycoproteins					62:74	Panax ginseng glycoproteins	48:74	Panax ginseng glycoproteins	48:74	In vivo and in vitro neuroprotective effects of Panax ginseng glycoproteins.
29408615	0	77	dep	In	0:1	arg1	vivo					3:6	vivo	3:6	vivo	3:6	In vivo and in vitro neuroprotective effects of Panax ginseng glycoproteins.
29408615	2	78	theme	macroporous	326:336	arg1	ultrafiltration					358:372	a macroporous resin, hollow-fiber ultrafiltration	324:372	ultrafiltration	358:372	A glycoproteins named PGL-1 was extracted from ginseng which subjected to through a macroporous resin, hollow-fiber ultrafiltration and dialyzed.
29408615	8	79	theme	significant	1098:1108	arg1	activity					1110:1117	significant activity	1098:1117	significant activity of protective effect on apoptosis SH-SY5Y induced by Aβ25-35	1098:1178	All dose groups showed significant activity of protective effect on apoptosis SH-SY5Y induced by Aβ25-35, and obviously inhibited the S phase arrest.
31369799	0	0	theme	pituitary	104:112	arg1	cells					134:138	mouse pituitary corticotroph AtT-20 cells	98:138	mouse pituitary corticotroph AtT-20 cells	98:138	O-linked β-N-acetylglucosamine transferase is involved in pro-opiomelanocortin gene expression in mouse pituitary corticotroph AtT-20 cells.
31369799	0	1	theme	mouse	98:102	arg1	cells					134:138	mouse pituitary corticotroph AtT-20 cells	98:138	mouse pituitary corticotroph AtT-20 cells	98:138	O-linked β-N-acetylglucosamine transferase is involved in pro-opiomelanocortin gene expression in mouse pituitary corticotroph AtT-20 cells.
31369799	8	2	theme	transcription	1087:1099	arg1	regulation					1068:1077	the negative regulation	1055:1077	the negative regulation of Pomc transcription in corticotroph cells	1055:1121	O-GlcNAc modification is involved in the negative regulation of Pomc transcription in corticotroph cells.
31369799	7	3	theme	polymerase	985:994	arg1	recruitment					929:939	the decreased recruitment	915:939	the decreased recruitment of GRs	915:946	OGT inhibition leads to both the decreased recruitment of GRs and the increased recruitment of RNA polymerase II to the Pomc locus.
31369799	7	3	theme	polymerase	985:994	arg1	recruitment					966:976	the increased recruitment	952:976	the increased recruitment of RNA polymerase II to the Pomc locus	952:1015	OGT inhibition leads to both the decreased recruitment of GRs and the increased recruitment of RNA polymerase II to the Pomc locus.
31369799	2	4	theme	gene	383:386	arg1	transcription					388:400	gene transcription	383:400	gene transcription	383:400	O-linked β-N-acetylglucosamine (O-GlcNAc) modification, mediated by O-GlcNAc transferase (OGT), plays an important role during gene transcription.
31369799	4	5	theme	cell	614:617	arg1	line					619:622	mouse corticotroph cell line	595:622	the mouse corticotroph cell line AtT-20	591:629	Here, we report that OGT regulates Pomc expression in the mouse corticotroph cell line AtT-20.
31369799	0	6	theme	AtT-20	127:132	arg1	cells					134:138	mouse pituitary corticotroph AtT-20 cells	98:138	mouse pituitary corticotroph AtT-20 cells	98:138	O-linked β-N-acetylglucosamine transferase is involved in pro-opiomelanocortin gene expression in mouse pituitary corticotroph AtT-20 cells.
31369799	5	7	theme	GR-mediated	688:698	arg1	pathway					723:729	the GR-mediated negative transcription pathway	684:729	the GR-mediated negative transcription pathway	684:729	The overexpression of OGT has an additive effect on the GR-mediated negative transcription pathway.
31369799	9	8	theme	disease	1197:1203	arg1	treatment					1174:1182	the treatment	1170:1182	the treatment of Cushing's disease	1170:1203	OGT may be a promising therapeutic target for the treatment of Cushing's disease.
31369799	8	9	from	regulation	1068:1077	arg1	cells					1117:1121	corticotroph cells	1104:1121	corticotroph cells	1104:1121	O-GlcNAc modification is involved in the negative regulation of Pomc transcription in corticotroph cells.
31369799	0	10	theme	corticotroph	114:125	arg1	cells					134:138	mouse pituitary corticotroph AtT-20 cells	98:138	mouse pituitary corticotroph AtT-20 cells	98:138	O-linked β-N-acetylglucosamine transferase is involved in pro-opiomelanocortin gene expression in mouse pituitary corticotroph AtT-20 cells.
31369799	5	11	theme	negative	700:707	arg1	pathway					723:729	the GR-mediated negative transcription pathway	684:729	the GR-mediated negative transcription pathway	684:729	The overexpression of OGT has an additive effect on the GR-mediated negative transcription pathway.
31369799	6	12	theme	repressive	832:841	arg1	effects					843:849	the repressive effects	828:849	the repressive effects of Pomc expression induced by GRs	828:883	Both the knockdown of OGT by RNA interference and the use of a chemical OGT inhibitor abolished the repressive effects of Pomc expression induced by GRs.
31369799	6	13	theme	OGT	754:756	arg1	knockdown					741:749	the knockdown	737:749	the knockdown of OGT by RNA interference	737:776	Both the knockdown of OGT by RNA interference and the use of a chemical OGT inhibitor abolished the repressive effects of Pomc expression induced by GRs.
31369799	6	13	theme	OGT	754:756	arg1	use					786:788	the use	782:788	the use of a chemical OGT inhibitor	782:816	Both the knockdown of OGT by RNA interference and the use of a chemical OGT inhibitor abolished the repressive effects of Pomc expression induced by GRs.
31369799	5	14	contain	has	658:660	arg1	overexpression					636:649	The overexpression	632:649	The overexpression of OGT	632:656	The overexpression of OGT has an additive effect on the GR-mediated negative transcription pathway.
31369799	5	14	contain	has	658:660	arg2	effect					674:679	an additive effect	662:679	an additive effect	662:679	The overexpression of OGT has an additive effect on the GR-mediated negative transcription pathway.
31369799	7	15	theme	OGT	886:888	arg1	inhibition					890:899	OGT inhibition	886:899	OGT inhibition	886:899	OGT inhibition leads to both the decreased recruitment of GRs and the increased recruitment of RNA polymerase II to the Pomc locus.
31369799	0	16	link	O-linked	0:7	arg1	transferase					31:41	O-linked β-N-acetylglucosamine transferase	0:41	O-linked β-N-acetylglucosamine transferase	0:41	O-linked β-N-acetylglucosamine transferase is involved in pro-opiomelanocortin gene expression in mouse pituitary corticotroph AtT-20 cells.
31369799	2	17	theme	O-GlcNAc	324:331	arg1	transferase					333:343	O-GlcNAc transferase	324:343	O-GlcNAc transferase (OGT)	324:349	O-linked β-N-acetylglucosamine (O-GlcNAc) modification, mediated by O-GlcNAc transferase (OGT), plays an important role during gene transcription.
31369799	2	17	theme	O-GlcNAc	324:331	arg1	OGT					346:348	OGT	346:348	OGT	346:348	O-linked β-N-acetylglucosamine (O-GlcNAc) modification, mediated by O-GlcNAc transferase (OGT), plays an important role during gene transcription.
31369799	1	18	theme	pituitary	201:209	arg1	expression					244:253	pituitary pro-opiomelanocortin (Pomc) gene expression	201:253	pituitary pro-opiomelanocortin (Pomc) gene expression	201:253	Glucocorticoids and glucocorticoid receptors (GRs) suppress pituitary pro-opiomelanocortin (Pomc) gene expression.
31369799	7	19	theme	increased	956:964	arg1	recruitment					966:976	the increased recruitment	952:976	the increased recruitment of RNA polymerase II to the Pomc locus	952:1015	OGT inhibition leads to both the decreased recruitment of GRs and the increased recruitment of RNA polymerase II to the Pomc locus.
31369799	2	20	link	O-linked	256:263	arg1	modification					298:309	O-linked β-N-acetylglucosamine (O-GlcNAc) modification	256:309	O-linked β-N-acetylglucosamine (O-GlcNAc) modification	256:309	O-linked β-N-acetylglucosamine (O-GlcNAc) modification, mediated by O-GlcNAc transferase (OGT), plays an important role during gene transcription.
31369799	1	21	theme	pro-opiomelanocortin	211:230	arg1	expression					244:253	pituitary pro-opiomelanocortin (Pomc) gene expression	201:253	pituitary pro-opiomelanocortin (Pomc) gene expression	201:253	Glucocorticoids and glucocorticoid receptors (GRs) suppress pituitary pro-opiomelanocortin (Pomc) gene expression.
31369799	0	22	theme	β-N-acetylglucosamine	9:29	arg1	transferase					31:41	O-linked β-N-acetylglucosamine transferase	0:41	O-linked β-N-acetylglucosamine transferase	0:41	O-linked β-N-acetylglucosamine transferase is involved in pro-opiomelanocortin gene expression in mouse pituitary corticotroph AtT-20 cells.
31369799	5	23	theme	additive	665:672	arg1	effect					674:679	an additive effect	662:679	an additive effect	662:679	The overexpression of OGT has an additive effect on the GR-mediated negative transcription pathway.
31369799	6	24	theme	inhibitor	808:816	arg1	knockdown					741:749	the knockdown	737:749	the knockdown of OGT by RNA interference	737:776	Both the knockdown of OGT by RNA interference and the use of a chemical OGT inhibitor abolished the repressive effects of Pomc expression induced by GRs.
31369799	6	24	theme	inhibitor	808:816	arg1	use					786:788	the use	782:788	the use of a chemical OGT inhibitor	782:816	Both the knockdown of OGT by RNA interference and the use of a chemical OGT inhibitor abolished the repressive effects of Pomc expression induced by GRs.
31369799	0	25	theme	O-linked	0:7	arg1	transferase					31:41	O-linked β-N-acetylglucosamine transferase	0:41	O-linked β-N-acetylglucosamine transferase	0:41	O-linked β-N-acetylglucosamine transferase is involved in pro-opiomelanocortin gene expression in mouse pituitary corticotroph AtT-20 cells.
31369799	8	26	theme	Pomc	1082:1085	arg1	transcription					1087:1099	Pomc transcription	1082:1099	Pomc transcription	1082:1099	O-GlcNAc modification is involved in the negative regulation of Pomc transcription in corticotroph cells.
31369799	5	27	theme	OGT	654:656	arg1	overexpression					636:649	The overexpression	632:649	The overexpression of OGT	632:656	The overexpression of OGT has an additive effect on the GR-mediated negative transcription pathway.
31369799	6	28	theme	OGT	804:806	arg1	inhibitor					808:816	a chemical OGT inhibitor	793:816	a chemical OGT inhibitor	793:816	Both the knockdown of OGT by RNA interference and the use of a chemical OGT inhibitor abolished the repressive effects of Pomc expression induced by GRs.
31369799	8	29	theme	corticotroph	1104:1115	arg1	cells					1117:1121	corticotroph cells	1104:1121	corticotroph cells	1104:1121	O-GlcNAc modification is involved in the negative regulation of Pomc transcription in corticotroph cells.
31369799	2	30	theme	O-linked	256:263	arg1	modification					298:309	O-linked β-N-acetylglucosamine (O-GlcNAc) modification	256:309	O-linked β-N-acetylglucosamine (O-GlcNAc) modification	256:309	O-linked β-N-acetylglucosamine (O-GlcNAc) modification, mediated by O-GlcNAc transferase (OGT), plays an important role during gene transcription.
31369799	4	31	theme	corticotroph	601:612	arg1	line					619:622	mouse corticotroph cell line	595:622	the mouse corticotroph cell line AtT-20	591:629	Here, we report that OGT regulates Pomc expression in the mouse corticotroph cell line AtT-20.
31369799	7	32	theme	RNA	981:983	arg1	polymerase					985:994	RNA polymerase II	981:997	RNA polymerase II	981:997	OGT inhibition leads to both the decreased recruitment of GRs and the increased recruitment of RNA polymerase II to the Pomc locus.
31369799	1	33	theme	gene	239:242	arg1	expression					244:253	pituitary pro-opiomelanocortin (Pomc) gene expression	201:253	pituitary pro-opiomelanocortin (Pomc) gene expression	201:253	Glucocorticoids and glucocorticoid receptors (GRs) suppress pituitary pro-opiomelanocortin (Pomc) gene expression.
31369799	4	34	from	expression	577:586	arg1	line					619:622	mouse corticotroph cell line	595:622	the mouse corticotroph cell line AtT-20	591:629	Here, we report that OGT regulates Pomc expression in the mouse corticotroph cell line AtT-20.
31369799	4	35	theme	mouse	595:599	arg1	line					619:622	mouse corticotroph cell line	595:622	the mouse corticotroph cell line AtT-20	591:629	Here, we report that OGT regulates Pomc expression in the mouse corticotroph cell line AtT-20.
31369799	2	36	theme	O-GlcNAc	288:295	arg1	modification					298:309	O-linked β-N-acetylglucosamine (O-GlcNAc) modification	256:309	O-linked β-N-acetylglucosamine (O-GlcNAc) modification	256:309	O-linked β-N-acetylglucosamine (O-GlcNAc) modification, mediated by O-GlcNAc transferase (OGT), plays an important role during gene transcription.
31369799	3	37	theme	pituitary	486:494	arg1	cells					509:513	pituitary corticotroph cells	486:513	pituitary corticotroph cells	486:513	However, whether OGT is involved in the GR-mediated transrepression that occurs in pituitary corticotroph cells is currently unknown.
31369799	3	38	theme	GR-mediated	443:453	arg1	transrepression					455:469	the GR-mediated transrepression	439:469	the GR-mediated transrepression that occurs in pituitary corticotroph cells	439:513	However, whether OGT is involved in the GR-mediated transrepression that occurs in pituitary corticotroph cells is currently unknown.
31369799	4	39	dep	line	619:622	arg1	AtT-20					624:629	AtT-20	624:629	the mouse corticotroph cell line AtT-20	591:629	Here, we report that OGT regulates Pomc expression in the mouse corticotroph cell line AtT-20.
31369799	9	40	theme	promising	1137:1145	arg1	target					1159:1164	a promising therapeutic target	1135:1164	a promising therapeutic target for the treatment of Cushing's disease	1135:1203	OGT may be a promising therapeutic target for the treatment of Cushing's disease.
31369799	9	40	theme	promising	1137:1145	arg1	OGT					1124:1126	OGT	1124:1126	OGT	1124:1126	OGT may be a promising therapeutic target for the treatment of Cushing's disease.
31369799	3	41	theme	corticotroph	496:507	arg1	cells					509:513	pituitary corticotroph cells	486:513	pituitary corticotroph cells	486:513	However, whether OGT is involved in the GR-mediated transrepression that occurs in pituitary corticotroph cells is currently unknown.
31369799	0	42	theme	gene	79:82	arg1	expression					84:93	pro-opiomelanocortin gene expression	58:93	pro-opiomelanocortin gene expression in mouse pituitary corticotroph AtT-20 cells	58:138	O-linked β-N-acetylglucosamine transferase is involved in pro-opiomelanocortin gene expression in mouse pituitary corticotroph AtT-20 cells.
31369799	5	43	theme	transcription	709:721	arg1	pathway					723:729	the GR-mediated negative transcription pathway	684:729	the GR-mediated negative transcription pathway	684:729	The overexpression of OGT has an additive effect on the GR-mediated negative transcription pathway.
31369799	0	44	from	expression	84:93	arg1	cells					134:138	mouse pituitary corticotroph AtT-20 cells	98:138	mouse pituitary corticotroph AtT-20 cells	98:138	O-linked β-N-acetylglucosamine transferase is involved in pro-opiomelanocortin gene expression in mouse pituitary corticotroph AtT-20 cells.
31369799	2	45	theme	β-N-acetylglucosamine	265:285	arg1	modification					298:309	O-linked β-N-acetylglucosamine (O-GlcNAc) modification	256:309	O-linked β-N-acetylglucosamine (O-GlcNAc) modification	256:309	O-linked β-N-acetylglucosamine (O-GlcNAc) modification, mediated by O-GlcNAc transferase (OGT), plays an important role during gene transcription.
31369799	6	46	theme	chemical	795:802	arg1	inhibitor					808:816	a chemical OGT inhibitor	793:816	a chemical OGT inhibitor	793:816	Both the knockdown of OGT by RNA interference and the use of a chemical OGT inhibitor abolished the repressive effects of Pomc expression induced by GRs.
31369799	0	47	theme	pro-opiomelanocortin	58:77	arg1	expression					84:93	pro-opiomelanocortin gene expression	58:93	pro-opiomelanocortin gene expression in mouse pituitary corticotroph AtT-20 cells	58:138	O-linked β-N-acetylglucosamine transferase is involved in pro-opiomelanocortin gene expression in mouse pituitary corticotroph AtT-20 cells.
31369799	1	48	dep	pro-opiomelanocortin	211:230	arg1	Pomc					233:236	Pomc	233:236	Pomc	233:236	Glucocorticoids and glucocorticoid receptors (GRs) suppress pituitary pro-opiomelanocortin (Pomc) gene expression.
31369799	8	49	theme	negative	1059:1066	arg1	regulation					1068:1077	the negative regulation	1055:1077	the negative regulation of Pomc transcription in corticotroph cells	1055:1121	O-GlcNAc modification is involved in the negative regulation of Pomc transcription in corticotroph cells.
31369799	4	50	theme	Pomc	572:575	arg1	expression					577:586	Pomc expression	572:586	Pomc expression in the mouse corticotroph cell line AtT-20	572:629	Here, we report that OGT regulates Pomc expression in the mouse corticotroph cell line AtT-20.
31369799	8	51	theme	O-GlcNAc	1018:1025	arg1	modification					1027:1038	O-GlcNAc modification	1018:1038	O-GlcNAc modification	1018:1038	O-GlcNAc modification is involved in the negative regulation of Pomc transcription in corticotroph cells.
31369799	1	52	theme	glucocorticoid	161:174	arg1	GRs					187:189	GRs	187:189	GRs	187:189	Glucocorticoids and glucocorticoid receptors (GRs) suppress pituitary pro-opiomelanocortin (Pomc) gene expression.
31369799	1	52	theme	glucocorticoid	161:174	arg1	receptors					176:184	glucocorticoid receptors	161:184	glucocorticoid receptors (GRs)	161:190	Glucocorticoids and glucocorticoid receptors (GRs) suppress pituitary pro-opiomelanocortin (Pomc) gene expression.
31369799	7	53	theme	GRs	944:946	arg1	recruitment					929:939	the decreased recruitment	915:939	the decreased recruitment of GRs	915:946	OGT inhibition leads to both the decreased recruitment of GRs and the increased recruitment of RNA polymerase II to the Pomc locus.
31369799	7	53	theme	GRs	944:946	arg1	recruitment					966:976	the increased recruitment	952:976	the increased recruitment of RNA polymerase II to the Pomc locus	952:1015	OGT inhibition leads to both the decreased recruitment of GRs and the increased recruitment of RNA polymerase II to the Pomc locus.
31369799	9	54	theme	therapeutic	1147:1157	arg1	target					1159:1164	a promising therapeutic target	1135:1164	a promising therapeutic target for the treatment of Cushing's disease	1135:1203	OGT may be a promising therapeutic target for the treatment of Cushing's disease.
31369799	9	54	theme	therapeutic	1147:1157	arg1	OGT					1124:1126	OGT	1124:1126	OGT	1124:1126	OGT may be a promising therapeutic target for the treatment of Cushing's disease.
31369799	6	55	theme	expression	859:868	arg1	effects					843:849	the repressive effects	828:849	the repressive effects of Pomc expression induced by GRs	828:883	Both the knockdown of OGT by RNA interference and the use of a chemical OGT inhibitor abolished the repressive effects of Pomc expression induced by GRs.
31369799	7	56	theme	Pomc	1006:1009	arg1	locus					1011:1015	the Pomc locus	1002:1015	the Pomc locus	1002:1015	OGT inhibition leads to both the decreased recruitment of GRs and the increased recruitment of RNA polymerase II to the Pomc locus.
31369799	7	57	theme	decreased	919:927	arg1	recruitment					929:939	the decreased recruitment	915:939	the decreased recruitment of GRs	915:946	OGT inhibition leads to both the decreased recruitment of GRs and the increased recruitment of RNA polymerase II to the Pomc locus.
31369799	2	58	theme	important	361:369	arg1	role					371:374	an important role	358:374	an important role	358:374	O-linked β-N-acetylglucosamine (O-GlcNAc) modification, mediated by O-GlcNAc transferase (OGT), plays an important role during gene transcription.
31369799	6	59	theme	Pomc	854:857	arg1	expression					859:868	Pomc expression	854:868	Pomc expression induced by GRs	854:883	Both the knockdown of OGT by RNA interference and the use of a chemical OGT inhibitor abolished the repressive effects of Pomc expression induced by GRs.
31369799	6	60	theme	RNA	761:763	arg1	interference					765:776	RNA interference	761:776	RNA interference	761:776	Both the knockdown of OGT by RNA interference and the use of a chemical OGT inhibitor abolished the repressive effects of Pomc expression induced by GRs.
31091698	7	0	theme	desired	1046:1052	arg1	value					1054:1058	a desired value	1044:1058	a desired value of 0.381	1044:1067	A response surface methodology based on a Box-Behnken design (BBD) was used to optimize the extraction conditions as follows: extraction time of 43.63 min, power level of 600 W, water-to-raw-material ratio of 55.45, pH of 6.57, and maximum yield of 41.91%, with a desired value of 0.381.
31091698	7	1	theme	extraction	874:883	arg1	conditions					885:894	the extraction conditions	870:894	the extraction conditions	870:894	A response surface methodology based on a Box-Behnken design (BBD) was used to optimize the extraction conditions as follows: extraction time of 43.63 min, power level of 600 W, water-to-raw-material ratio of 55.45, pH of 6.57, and maximum yield of 41.91%, with a desired value of 0.381.
31091698	8	2	theme	3-ethylbenzothiazoline-6-sulphonic	1176:1209	arg1	2,2'-azino-bis					1161:1174	2,2'-azino-bis	1161:1174	2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS)	1161:1222	Ulvan exerted a strong antioxidant effect against 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and showed reducing power in vitro.
31091698	8	2	theme	3-ethylbenzothiazoline-6-sulphonic	1176:1209	arg1	acid					1211:1214	3-ethylbenzothiazoline-6-sulphonic acid	1176:1214	3-ethylbenzothiazoline-6-sulphonic acid	1176:1214	Ulvan exerted a strong antioxidant effect against 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and showed reducing power in vitro.
31091698	5	3	dep	700	550:552	arg1	to					547:548	to	547:548	to	547:548	The impacts of four independent variables, i.e., extraction time (X1, 30 to 60 min), power (X2, 500 to 700 W), water-to-raw-material ratio (X3, 40 to 70), and pH (X4, 5 to 7) were evaluated.
31091698	9	4	theme	oxidative	1313:1321	arg1	stress					1323:1328	H2O2-induced oxidative stress	1300:1328	H2O2-induced oxidative stress	1300:1328	Ulvan protected RAW 264.7 cells against H2O2-induced oxidative stress by upregulating the expression and enhancing the activity of oxidative enzymes such as superoxide dismutase (SOD) and superoxide dismutase (CAT).
31091698	9	5	theme	enzymes	1401:1407	arg1	activity					1379:1386	the activity	1375:1386	the activity of oxidative enzymes such as superoxide dismutase (SOD) and superoxide dismutase (CAT)	1375:1473	Ulvan protected RAW 264.7 cells against H2O2-induced oxidative stress by upregulating the expression and enhancing the activity of oxidative enzymes such as superoxide dismutase (SOD) and superoxide dismutase (CAT).
31091698	0	6	theme	Antioxidant	107:117	arg1	Activity					119:126	Their Antioxidant Activity	101:126	Their Antioxidant Activity	101:126	Optimization of Microwave-Assisted Extraction of Polysaccharides from Ulva pertusa and Evaluation of Their Antioxidant Activity.
31091698	4	7	theme	ulvan	421:425	arg1	activity					409:416	The antioxidant activity	393:416	The antioxidant activity of ulvan	393:425	The antioxidant activity of ulvan was also evaluated.
31091698	7	8	theme	response	784:791	arg1	methodology					801:811	A response surface methodology	782:811	A response surface methodology based on a Box-Behnken design (BBD)	782:847	A response surface methodology based on a Box-Behnken design (BBD) was used to optimize the extraction conditions as follows: extraction time of 43.63 min, power level of 600 W, water-to-raw-material ratio of 55.45, pH of 6.57, and maximum yield of 41.91%, with a desired value of 0.381.
31091698	9	9	theme	RAW	1276:1278	arg1	cells					1286:1290	RAW 264.7 cells	1276:1290	RAW 264.7 cells	1276:1290	Ulvan protected RAW 264.7 cells against H2O2-induced oxidative stress by upregulating the expression and enhancing the activity of oxidative enzymes such as superoxide dismutase (SOD) and superoxide dismutase (CAT).
31091698	0	10	from	Ulva	70:73	arg1	Polysaccharides					49:63	Polysaccharides	49:63	Polysaccharides from Ulva pertusa and Evaluation of Their Antioxidant Activity	49:126	Optimization of Microwave-Assisted Extraction of Polysaccharides from Ulva pertusa and Evaluation of Their Antioxidant Activity.
31091698	0	10	from	Ulva	70:73	arg1	Extraction					35:44	Microwave-Assisted Extraction	16:44	Microwave-Assisted Extraction of Polysaccharides from Ulva pertusa and Evaluation of Their Antioxidant Activity	16:126	Optimization of Microwave-Assisted Extraction of Polysaccharides from Ulva pertusa and Evaluation of Their Antioxidant Activity.
31091698	7	11	theme	55.45	991:995	arg1	ratio					982:986	water-to-raw-material ratio	960:986	water-to-raw-material ratio of 55.45	960:995	A response surface methodology based on a Box-Behnken design (BBD) was used to optimize the extraction conditions as follows: extraction time of 43.63 min, power level of 600 W, water-to-raw-material ratio of 55.45, pH of 6.57, and maximum yield of 41.91%, with a desired value of 0.381.
31091698	9	12	theme	oxidative	1391:1399	arg1	dismutase					1428:1436	superoxide dismutase	1417:1436	superoxide dismutase (SOD)	1417:1442	Ulvan protected RAW 264.7 cells against H2O2-induced oxidative stress by upregulating the expression and enhancing the activity of oxidative enzymes such as superoxide dismutase (SOD) and superoxide dismutase (CAT).
31091698	9	12	theme	oxidative	1391:1399	arg1	dismutase					1459:1467	superoxide dismutase	1448:1467	superoxide dismutase (CAT)	1448:1473	Ulvan protected RAW 264.7 cells against H2O2-induced oxidative stress by upregulating the expression and enhancing the activity of oxidative enzymes such as superoxide dismutase (SOD) and superoxide dismutase (CAT).
31091698	9	12	theme	oxidative	1391:1399	arg1	enzymes					1401:1407	oxidative enzymes	1391:1407	oxidative enzymes such as superoxide dismutase (SOD) and superoxide dismutase (CAT)	1391:1473	Ulvan protected RAW 264.7 cells against H2O2-induced oxidative stress by upregulating the expression and enhancing the activity of oxidative enzymes such as superoxide dismutase (SOD) and superoxide dismutase (CAT).
31091698	7	13	used	used	853:856	arg2	methodology					801:811	A response surface methodology	782:811	A response surface methodology based on a Box-Behnken design (BBD)	782:847	A response surface methodology based on a Box-Behnken design (BBD) was used to optimize the extraction conditions as follows: extraction time of 43.63 min, power level of 600 W, water-to-raw-material ratio of 55.45, pH of 6.57, and maximum yield of 41.91%, with a desired value of 0.381.
31091698	3	14	theme	Ulva	363:366	arg1	polysaccharides					376:390	Ulva pertusa polysaccharides	363:390	Ulva pertusa polysaccharides	363:390	Microwave-assisted extraction technology was employed to improve the extraction yield and composition of Ulva pertusa polysaccharides.
31091698	7	15	theme	water-to-raw-material	960:980	arg1	ratio					982:986	water-to-raw-material ratio	960:986	water-to-raw-material ratio of 55.45	960:995	A response surface methodology based on a Box-Behnken design (BBD) was used to optimize the extraction conditions as follows: extraction time of 43.63 min, power level of 600 W, water-to-raw-material ratio of 55.45, pH of 6.57, and maximum yield of 41.91%, with a desired value of 0.381.
31091698	5	16	theme	water-to-raw-material	558:578	arg1	ratio					580:584	water-to-raw-material ratio	558:584	water-to-raw-material ratio (X3, 40 to 70)	558:599	The impacts of four independent variables, i.e., extraction time (X1, 30 to 60 min), power (X2, 500 to 700 W), water-to-raw-material ratio (X3, 40 to 70), and pH (X4, 5 to 7) were evaluated.
31091698	9	17	theme	superoxide	1417:1426	arg1	SOD					1439:1441	SOD	1439:1441	SOD	1439:1441	Ulvan protected RAW 264.7 cells against H2O2-induced oxidative stress by upregulating the expression and enhancing the activity of oxidative enzymes such as superoxide dismutase (SOD) and superoxide dismutase (CAT).
31091698	9	17	theme	superoxide	1417:1426	arg1	dismutase					1428:1436	superoxide dismutase	1417:1436	superoxide dismutase (SOD)	1417:1442	Ulvan protected RAW 264.7 cells against H2O2-induced oxidative stress by upregulating the expression and enhancing the activity of oxidative enzymes such as superoxide dismutase (SOD) and superoxide dismutase (CAT).
31091698	5	18	dep	X2	539:540	arg1	W					554:554	500 to 700 W	543:554	500 to 700 W	543:554	The impacts of four independent variables, i.e., extraction time (X1, 30 to 60 min), power (X2, 500 to 700 W), water-to-raw-material ratio (X3, 40 to 70), and pH (X4, 5 to 7) were evaluated.
31091698	0	19	theme	Activity	119:126	arg1	Evaluation					87:96	Evaluation	87:96	Evaluation of Their Antioxidant Activity	87:126	Optimization of Microwave-Assisted Extraction of Polysaccharides from Ulva pertusa and Evaluation of Their Antioxidant Activity.
31091698	3	20	theme	Microwave-assisted	258:275	arg1	technology					288:297	Microwave-assisted extraction technology	258:297	Microwave-assisted extraction technology	258:297	Microwave-assisted extraction technology was employed to improve the extraction yield and composition of Ulva pertusa polysaccharides.
31091698	4	21	theme	antioxidant	397:407	arg1	activity					409:416	The antioxidant activity	393:416	The antioxidant activity of ulvan	393:425	The antioxidant activity of ulvan was also evaluated.
31091698	7	22	theme	%	1036:1036	arg1	yield					1022:1026	maximum yield	1014:1026	maximum yield of 41.91%	1014:1036	A response surface methodology based on a Box-Behnken design (BBD) was used to optimize the extraction conditions as follows: extraction time of 43.63 min, power level of 600 W, water-to-raw-material ratio of 55.45, pH of 6.57, and maximum yield of 41.91%, with a desired value of 0.381.
31091698	5	23	dep	70	597:598	arg1	to					594:595	to	594:595	to	594:595	The impacts of four independent variables, i.e., extraction time (X1, 30 to 60 min), power (X2, 500 to 700 W), water-to-raw-material ratio (X3, 40 to 70), and pH (X4, 5 to 7) were evaluated.
31091698	7	24	theme	W	957:957	arg1	level					944:948	power level	938:948	power level of 600 W	938:957	A response surface methodology based on a Box-Behnken design (BBD) was used to optimize the extraction conditions as follows: extraction time of 43.63 min, power level of 600 W, water-to-raw-material ratio of 55.45, pH of 6.57, and maximum yield of 41.91%, with a desired value of 0.381.
31091698	6	25	theme	polysaccharides	674:688	arg1	fractions					690:698	different polysaccharides fractions	664:698	different polysaccharides fractions	664:698	The chemical structure of different polysaccharides fractions was investigated via FT-IR and the determination of their antioxidant activities.
31091698	9	26	theme	H2O2-induced	1300:1311	arg1	stress					1323:1328	H2O2-induced oxidative stress	1300:1328	H2O2-induced oxidative stress	1300:1328	Ulvan protected RAW 264.7 cells against H2O2-induced oxidative stress by upregulating the expression and enhancing the activity of oxidative enzymes such as superoxide dismutase (SOD) and superoxide dismutase (CAT).
31091698	7	27	theme	power	938:942	arg1	level					944:948	power level	938:948	power level of 600 W	938:957	A response surface methodology based on a Box-Behnken design (BBD) was used to optimize the extraction conditions as follows: extraction time of 43.63 min, power level of 600 W, water-to-raw-material ratio of 55.45, pH of 6.57, and maximum yield of 41.91%, with a desired value of 0.381.
31091698	6	28	theme	different	664:672	arg1	fractions					690:698	different polysaccharides fractions	664:698	different polysaccharides fractions	664:698	The chemical structure of different polysaccharides fractions was investigated via FT-IR and the determination of their antioxidant activities.
31091698	6	29	theme	activities	770:779	arg1	determination					735:747	the determination	731:747	the determination of their antioxidant activities	731:779	The chemical structure of different polysaccharides fractions was investigated via FT-IR and the determination of their antioxidant activities.
31091698	6	29	theme	activities	770:779	arg1	FT-IR					721:725	FT-IR	721:725	FT-IR	721:725	The chemical structure of different polysaccharides fractions was investigated via FT-IR and the determination of their antioxidant activities.
31091698	0	30	theme	Extraction	35:44	arg1	Optimization					0:11	Optimization	0:11	Optimization of Microwave-Assisted Extraction of Polysaccharides from Ulva pertusa and Evaluation of Their Antioxidant Activity.	0:127	Optimization of Microwave-Assisted Extraction of Polysaccharides from Ulva pertusa and Evaluation of Their Antioxidant Activity.
31091698	8	31	theme	reducing	1235:1242	arg1	power					1244:1248	reducing power	1235:1248	reducing power	1235:1248	Ulvan exerted a strong antioxidant effect against 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and showed reducing power in vitro.
31091698	9	32	theme	superoxide	1448:1457	arg1	CAT					1470:1472	CAT	1470:1472	CAT	1470:1472	Ulvan protected RAW 264.7 cells against H2O2-induced oxidative stress by upregulating the expression and enhancing the activity of oxidative enzymes such as superoxide dismutase (SOD) and superoxide dismutase (CAT).
31091698	9	32	theme	superoxide	1448:1457	arg1	dismutase					1459:1467	superoxide dismutase	1448:1467	superoxide dismutase (CAT)	1448:1473	Ulvan protected RAW 264.7 cells against H2O2-induced oxidative stress by upregulating the expression and enhancing the activity of oxidative enzymes such as superoxide dismutase (SOD) and superoxide dismutase (CAT).
31091698	8	33	theme	antioxidant	1093:1103	arg1	effect					1105:1110	a strong antioxidant effect	1084:1110	a strong antioxidant effect	1084:1110	Ulvan exerted a strong antioxidant effect against 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and showed reducing power in vitro.
31091698	0	34	theme	Microwave-Assisted	16:33	arg1	Extraction					35:44	Microwave-Assisted Extraction	16:44	Microwave-Assisted Extraction of Polysaccharides from Ulva pertusa and Evaluation of Their Antioxidant Activity	16:126	Optimization of Microwave-Assisted Extraction of Polysaccharides from Ulva pertusa and Evaluation of Their Antioxidant Activity.
31091698	5	35	theme	extraction	496:505	arg1	time					507:510	extraction time	496:510	extraction time (X1, 30 to 60 min)	496:529	The impacts of four independent variables, i.e., extraction time (X1, 30 to 60 min), power (X2, 500 to 700 W), water-to-raw-material ratio (X3, 40 to 70), and pH (X4, 5 to 7) were evaluated.
31091698	8	36	theme	strong	1086:1091	arg1	effect					1105:1110	a strong antioxidant effect	1084:1110	a strong antioxidant effect	1084:1110	Ulvan exerted a strong antioxidant effect against 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and showed reducing power in vitro.
31091698	0	37	theme	Polysaccharides	49:63	arg1	Extraction					35:44	Microwave-Assisted Extraction	16:44	Microwave-Assisted Extraction of Polysaccharides from Ulva pertusa and Evaluation of Their Antioxidant Activity	16:126	Optimization of Microwave-Assisted Extraction of Polysaccharides from Ulva pertusa and Evaluation of Their Antioxidant Activity.
31091698	3	38	theme	extraction	277:286	arg1	technology					288:297	Microwave-assisted extraction technology	258:297	Microwave-assisted extraction technology	258:297	Microwave-assisted extraction technology was employed to improve the extraction yield and composition of Ulva pertusa polysaccharides.
31091698	0	39	from	Evaluation	87:96	arg1	Polysaccharides					49:63	Polysaccharides	49:63	Polysaccharides from Ulva pertusa and Evaluation of Their Antioxidant Activity	49:126	Optimization of Microwave-Assisted Extraction of Polysaccharides from Ulva pertusa and Evaluation of Their Antioxidant Activity.
31091698	0	39	from	Evaluation	87:96	arg1	Extraction					35:44	Microwave-Assisted Extraction	16:44	Microwave-Assisted Extraction of Polysaccharides from Ulva pertusa and Evaluation of Their Antioxidant Activity	16:126	Optimization of Microwave-Assisted Extraction of Polysaccharides from Ulva pertusa and Evaluation of Their Antioxidant Activity.
31091698	0	40	dep	Ulva	70:73	arg1	pertusa					75:81	pertusa	75:81	pertusa	75:81	Optimization of Microwave-Assisted Extraction of Polysaccharides from Ulva pertusa and Evaluation of Their Antioxidant Activity.
31091698	5	41	dep	60	523:524	arg1	to					520:521	to	520:521	to	520:521	The impacts of four independent variables, i.e., extraction time (X1, 30 to 60 min), power (X2, 500 to 700 W), water-to-raw-material ratio (X3, 40 to 70), and pH (X4, 5 to 7) were evaluated.
31091698	5	42	dep	pH	606:607	arg1	X4					610:611	X4	610:611	X4	610:611	The impacts of four independent variables, i.e., extraction time (X1, 30 to 60 min), power (X2, 500 to 700 W), water-to-raw-material ratio (X3, 40 to 70), and pH (X4, 5 to 7) were evaluated.
31091698	6	43	theme	antioxidant	758:768	arg1	activities					770:779	their antioxidant activities	752:779	their antioxidant activities	752:779	The chemical structure of different polysaccharides fractions was investigated via FT-IR and the determination of their antioxidant activities.
31091698	7	44	theme	maximum	1014:1020	arg1	yield					1022:1026	maximum yield	1014:1026	maximum yield of 41.91%	1014:1036	A response surface methodology based on a Box-Behnken design (BBD) was used to optimize the extraction conditions as follows: extraction time of 43.63 min, power level of 600 W, water-to-raw-material ratio of 55.45, pH of 6.57, and maximum yield of 41.91%, with a desired value of 0.381.
31091698	7	45	theme	extraction	908:917	arg1	time					919:922	extraction time	908:922	extraction time of 43.63 min	908:935	A response surface methodology based on a Box-Behnken design (BBD) was used to optimize the extraction conditions as follows: extraction time of 43.63 min, power level of 600 W, water-to-raw-material ratio of 55.45, pH of 6.57, and maximum yield of 41.91%, with a desired value of 0.381.
31091698	5	46	dep	ratio	580:584	arg1	X3					587:588	X3	587:588	X3	587:588	The impacts of four independent variables, i.e., extraction time (X1, 30 to 60 min), power (X2, 500 to 700 W), water-to-raw-material ratio (X3, 40 to 70), and pH (X4, 5 to 7) were evaluated.
31091698	3	47	theme	polysaccharides	376:390	arg1	composition					348:358	composition	348:358	composition	348:358	Microwave-assisted extraction technology was employed to improve the extraction yield and composition of Ulva pertusa polysaccharides.
31091698	3	47	theme	polysaccharides	376:390	arg1	yield					338:342	extraction yield	327:342	extraction yield	327:342	Microwave-assisted extraction technology was employed to improve the extraction yield and composition of Ulva pertusa polysaccharides.
31091698	3	48	dep	Ulva	363:366	arg1	pertusa					368:374	pertusa	368:374	pertusa	368:374	Microwave-assisted extraction technology was employed to improve the extraction yield and composition of Ulva pertusa polysaccharides.
31091698	6	49	theme	chemical	642:649	arg1	structure					651:659	The chemical structure	638:659	The chemical structure of different polysaccharides fractions	638:698	The chemical structure of different polysaccharides fractions was investigated via FT-IR and the determination of their antioxidant activities.
31091698	7	50	theme	6.57	1004:1007	arg1	pH					998:999	pH	998:999	pH of 6.57	998:1007	A response surface methodology based on a Box-Behnken design (BBD) was used to optimize the extraction conditions as follows: extraction time of 43.63 min, power level of 600 W, water-to-raw-material ratio of 55.45, pH of 6.57, and maximum yield of 41.91%, with a desired value of 0.381.
31091698	3	51	theme	extraction	327:336	arg1	yield					338:342	extraction yield	327:342	extraction yield	327:342	Microwave-assisted extraction technology was employed to improve the extraction yield and composition of Ulva pertusa polysaccharides.
31091698	2	52	theme	functional	203:212	arg1	ingredients					214:224	functional ingredients	203:224	functional ingredients	203:224	as foods, feed supplements, and functional ingredients has gained increasing interest.
31091698	10	53	from	pertusa	1529:1535	arg1	polysaccharides					1505:1519	the polysaccharides	1501:1519	the polysaccharides from U. pertusa	1501:1535	The results suggest that the polysaccharides from U. pertusa might be promising bioactive compounds for commercial use.
31091698	10	53	from	pertusa	1529:1535	arg1	compounds					1566:1574	promising bioactive compounds	1546:1574	promising bioactive compounds	1546:1574	The results suggest that the polysaccharides from U. pertusa might be promising bioactive compounds for commercial use.
31091698	10	54	theme	bioactive	1556:1564	arg1	compounds					1566:1574	promising bioactive compounds	1546:1574	promising bioactive compounds	1546:1574	The results suggest that the polysaccharides from U. pertusa might be promising bioactive compounds for commercial use.
31091698	10	54	theme	bioactive	1556:1564	arg1	polysaccharides					1505:1519	the polysaccharides	1501:1519	the polysaccharides from U. pertusa	1501:1535	The results suggest that the polysaccharides from U. pertusa might be promising bioactive compounds for commercial use.
31091698	5	55	dep	7	619:619	arg1	to					616:617	to	616:617	to	616:617	The impacts of four independent variables, i.e., extraction time (X1, 30 to 60 min), power (X2, 500 to 700 W), water-to-raw-material ratio (X3, 40 to 70), and pH (X4, 5 to 7) were evaluated.
31091698	6	56	theme	fractions	690:698	arg1	structure					651:659	The chemical structure	638:659	The chemical structure of different polysaccharides fractions	638:698	The chemical structure of different polysaccharides fractions was investigated via FT-IR and the determination of their antioxidant activities.
31091698	10	57	theme	promising	1546:1554	arg1	compounds					1566:1574	promising bioactive compounds	1546:1574	promising bioactive compounds	1546:1574	The results suggest that the polysaccharides from U. pertusa might be promising bioactive compounds for commercial use.
31091698	10	57	theme	promising	1546:1554	arg1	polysaccharides					1505:1519	the polysaccharides	1501:1519	the polysaccharides from U. pertusa	1501:1535	The results suggest that the polysaccharides from U. pertusa might be promising bioactive compounds for commercial use.
31091698	5	58	theme	independent	467:477	arg1	variables					479:487	four independent variables	462:487	four independent variables	462:487	The impacts of four independent variables, i.e., extraction time (X1, 30 to 60 min), power (X2, 500 to 700 W), water-to-raw-material ratio (X3, 40 to 70), and pH (X4, 5 to 7) were evaluated.
31091698	10	59	theme	commercial	1580:1589	arg1	use					1591:1593	commercial use	1580:1593	commercial use	1580:1593	The results suggest that the polysaccharides from U. pertusa might be promising bioactive compounds for commercial use.
31091698	1	60	theme	green	140:144	arg1	marine					146:151	green marine	140:151	green marine	140:151	The use of green marine seaweed Ulva spp.
31091698	5	61	theme	variables	479:487	arg1	impacts					451:457	The impacts	447:457	The impacts of four independent variables, i.e., extraction time (X1, 30 to 60 min), power (X2, 500 to 700 W), water-to-raw-material ratio (X3, 40 to 70), and pH (X4, 5 to 7)	447:620	The impacts of four independent variables, i.e., extraction time (X1, 30 to 60 min), power (X2, 500 to 700 W), water-to-raw-material ratio (X3, 40 to 70), and pH (X4, 5 to 7) were evaluated.
31091698	2	62	theme	feed	181:184	arg1	supplements					186:196	feed supplements	181:196	feed supplements	181:196	as foods, feed supplements, and functional ingredients has gained increasing interest.
31091698	7	63	theme	min	933:935	arg1	time					919:922	extraction time	908:922	extraction time of 43.63 min	908:935	A response surface methodology based on a Box-Behnken design (BBD) was used to optimize the extraction conditions as follows: extraction time of 43.63 min, power level of 600 W, water-to-raw-material ratio of 55.45, pH of 6.57, and maximum yield of 41.91%, with a desired value of 0.381.
31091698	5	64	dep	time	507:510	arg1	min					526:528	30 to 60 min	517:528	30 to 60 min	517:528	The impacts of four independent variables, i.e., extraction time (X1, 30 to 60 min), power (X2, 500 to 700 W), water-to-raw-material ratio (X3, 40 to 70), and pH (X4, 5 to 7) were evaluated.
31091698	5	64	dep	time	507:510	arg1	X1					513:514	X1	513:514	X1	513:514	The impacts of four independent variables, i.e., extraction time (X1, 30 to 60 min), power (X2, 500 to 700 W), water-to-raw-material ratio (X3, 40 to 70), and pH (X4, 5 to 7) were evaluated.
31091698	5	64	dep	time	507:510	arg1	i.e.					490:493	i.e.	490:493	i.e.	490:493	The impacts of four independent variables, i.e., extraction time (X1, 30 to 60 min), power (X2, 500 to 700 W), water-to-raw-material ratio (X3, 40 to 70), and pH (X4, 5 to 7) were evaluated.
31091698	1	65	theme	marine	146:151	arg1	use					133:135	The use	129:135	The use of green marine	129:151	The use of green marine seaweed Ulva spp.
31091698	7	66	theme	Box-Behnken	824:834	arg1	BBD					844:846	BBD	844:846	BBD	844:846	A response surface methodology based on a Box-Behnken design (BBD) was used to optimize the extraction conditions as follows: extraction time of 43.63 min, power level of 600 W, water-to-raw-material ratio of 55.45, pH of 6.57, and maximum yield of 41.91%, with a desired value of 0.381.
31091698	7	66	theme	Box-Behnken	824:834	arg1	design					836:841	a Box-Behnken design	822:841	a Box-Behnken design (BBD)	822:847	A response surface methodology based on a Box-Behnken design (BBD) was used to optimize the extraction conditions as follows: extraction time of 43.63 min, power level of 600 W, water-to-raw-material ratio of 55.45, pH of 6.57, and maximum yield of 41.91%, with a desired value of 0.381.
31091698	7	67	theme	0.381	1063:1067	arg1	value					1054:1058	a desired value	1044:1058	a desired value of 0.381	1044:1067	A response surface methodology based on a Box-Behnken design (BBD) was used to optimize the extraction conditions as follows: extraction time of 43.63 min, power level of 600 W, water-to-raw-material ratio of 55.45, pH of 6.57, and maximum yield of 41.91%, with a desired value of 0.381.
31091698	0	68	from	Extraction	35:44	arg1	Evaluation					87:96	Evaluation	87:96	Evaluation of Their Antioxidant Activity	87:126	Optimization of Microwave-Assisted Extraction of Polysaccharides from Ulva pertusa and Evaluation of Their Antioxidant Activity.
31091698	0	68	from	Extraction	35:44	arg1	Ulva					70:73	Ulva	70:73	Ulva	70:73	Optimization of Microwave-Assisted Extraction of Polysaccharides from Ulva pertusa and Evaluation of Their Antioxidant Activity.
31091698	7	69	theme	surface	793:799	arg1	methodology					801:811	A response surface methodology	782:811	A response surface methodology based on a Box-Behnken design (BBD)	782:847	A response surface methodology based on a Box-Behnken design (BBD) was used to optimize the extraction conditions as follows: extraction time of 43.63 min, power level of 600 W, water-to-raw-material ratio of 55.45, pH of 6.57, and maximum yield of 41.91%, with a desired value of 0.381.
30177161	0	0	theme	binder	98:103	arg1	jetting					105:111	3D binder jetting	95:111	3D binder jetting	95:111	Selective recrystallization of cellulose composite powders and microstructure creation through 3D binder jetting.
30177161	2	1	theme	relevant	330:337	arg1	area					325:328	this area	320:328	this area relevant to food materials	320:355	Unfortunately work in this area relevant to food materials is largely unpublished, however a typical application of this technique is sugar powder bound by a water and alcohol based ink with optional colour or flavour demonstrated by commercial ventures.
30177161	4	2	theme	component	997:1005	arg1	proportion					972:981	a proportion	970:981	a proportion of the powder component (glucomannan)	970:1019	Powders comprising predominantly of ball milled, amorphous cellulose were successfully used to create 3D structures when interacting polysaccharides were present in the ink (xanthan gum) and as a proportion of the powder component (glucomannan) by inducing selective recrystallization.
30177161	0	3	theme	3D	95:96	arg1	jetting					105:111	3D binder jetting	95:111	3D binder jetting	95:111	Selective recrystallization of cellulose composite powders and microstructure creation through 3D binder jetting.
30177161	2	4	from	work	312:315	arg1	area					325:328	this area	320:328	this area relevant to food materials	320:355	Unfortunately work in this area relevant to food materials is largely unpublished, however a typical application of this technique is sugar powder bound by a water and alcohol based ink with optional colour or flavour demonstrated by commercial ventures.
30177161	1	5	theme	powdered	177:184	arg1	material					186:193	powdered material	177:193	powdered material	177:193	Binder jetting is an additive manufacturing technique in which powdered material is sequentially laid down and printed on by an ink binder, in a selective manner, to form a 3D object.
30177161	5	6	theme	food	1213:1216	arg1	products					1218:1225	food products	1213:1225	food products	1213:1225	These ingredients are categorized as dietary fibre, thus such formulations can be used to create low-calorie 3D printed food designs to be used within food products.
30177161	5	7	theme	low-calorie	1159:1169	arg1	designs					1187:1193	low-calorie 3D printed food designs	1159:1193	low-calorie 3D printed food designs	1159:1193	These ingredients are categorized as dietary fibre, thus such formulations can be used to create low-calorie 3D printed food designs to be used within food products.
30177161	3	8	theme	ink	638:640	arg1	printer					646:652	an ink jet printer	635:652	an ink jet printer to test prototype powders prior to producing quantities typically used in commercially available binder jetting machines	635:773	In this work we demonstrate the use of a small scale powder layering device under an ink jet printer to test prototype powders prior to producing quantities typically used in commercially available binder jetting machines.
30177161	4	9	used	used	863:866	arg2	Powders					776:782	Powders	776:782	Powders comprising predominantly of ball milled, amorphous cellulose	776:843	Powders comprising predominantly of ball milled, amorphous cellulose were successfully used to create 3D structures when interacting polysaccharides were present in the ink (xanthan gum) and as a proportion of the powder component (glucomannan) by inducing selective recrystallization.
30177161	3	10	theme	prototype	662:670	arg1	powders					672:678	test prototype powders	657:678	test prototype powders prior to producing quantities typically used in commercially available binder jetting machines	657:773	In this work we demonstrate the use of a small scale powder layering device under an ink jet printer to test prototype powders prior to producing quantities typically used in commercially available binder jetting machines.
30177161	1	11	theme	selective	259:267	arg1	manner					269:274	a selective manner	257:274	a selective manner	257:274	Binder jetting is an additive manufacturing technique in which powdered material is sequentially laid down and printed on by an ink binder, in a selective manner, to form a 3D object.
30177161	2	12	theme	based	474:478	arg1	ink					480:482	a water and alcohol based ink	454:482	ink	480:482	Unfortunately work in this area relevant to food materials is largely unpublished, however a typical application of this technique is sugar powder bound by a water and alcohol based ink with optional colour or flavour demonstrated by commercial ventures.
30177161	2	13	with	water	456:460	arg1	flavour					508:514	flavour	508:514	flavour	508:514	Unfortunately work in this area relevant to food materials is largely unpublished, however a typical application of this technique is sugar powder bound by a water and alcohol based ink with optional colour or flavour demonstrated by commercial ventures.
30177161	2	13	with	water	456:460	arg1	colour					498:503	optional colour	489:503	optional colour	489:503	Unfortunately work in this area relevant to food materials is largely unpublished, however a typical application of this technique is sugar powder bound by a water and alcohol based ink with optional colour or flavour demonstrated by commercial ventures.
30177161	2	14	theme	alcohol	466:472	arg1	ink					480:482	a water and alcohol based ink	454:482	ink	480:482	Unfortunately work in this area relevant to food materials is largely unpublished, however a typical application of this technique is sugar powder bound by a water and alcohol based ink with optional colour or flavour demonstrated by commercial ventures.
30177161	4	15	dep	milled	817:822	arg1	amorphous					825:833	amorphous	825:833	amorphous	825:833	Powders comprising predominantly of ball milled, amorphous cellulose were successfully used to create 3D structures when interacting polysaccharides were present in the ink (xanthan gum) and as a proportion of the powder component (glucomannan) by inducing selective recrystallization.
30177161	5	16	theme	food	1182:1185	arg1	designs					1187:1193	low-calorie 3D printed food designs	1159:1193	low-calorie 3D printed food designs	1159:1193	These ingredients are categorized as dietary fibre, thus such formulations can be used to create low-calorie 3D printed food designs to be used within food products.
30177161	2	17	theme	technique	419:427	arg1	application					399:409	a typical application	389:409	a typical application of this technique	389:427	Unfortunately work in this area relevant to food materials is largely unpublished, however a typical application of this technique is sugar powder bound by a water and alcohol based ink with optional colour or flavour demonstrated by commercial ventures.
30177161	2	17	theme	technique	419:427	arg1	powder					438:443	sugar powder	432:443	sugar powder bound by a water and alcohol based ink with optional colour or flavour demonstrated by commercial ventures	432:550	Unfortunately work in this area relevant to food materials is largely unpublished, however a typical application of this technique is sugar powder bound by a water and alcohol based ink with optional colour or flavour demonstrated by commercial ventures.
30177161	3	18	theme	test	657:660	arg1	powders					672:678	test prototype powders	657:678	test prototype powders prior to producing quantities typically used in commercially available binder jetting machines	657:773	In this work we demonstrate the use of a small scale powder layering device under an ink jet printer to test prototype powders prior to producing quantities typically used in commercially available binder jetting machines.
30177161	0	19	theme	Selective	0:8	arg1	recrystallization					10:26	Selective recrystallization	0:26	Selective recrystallization of cellulose composite powders and microstructure creation through 3D binder jetting.	0:112	Selective recrystallization of cellulose composite powders and microstructure creation through 3D binder jetting.
30177161	4	20	theme	milled	817:822	arg1	cellulose					835:843	ball milled, amorphous cellulose	812:843	ball milled, amorphous cellulose	812:843	Powders comprising predominantly of ball milled, amorphous cellulose were successfully used to create 3D structures when interacting polysaccharides were present in the ink (xanthan gum) and as a proportion of the powder component (glucomannan) by inducing selective recrystallization.
30177161	0	21	theme	cellulose	31:39	arg1	powders					51:57	cellulose composite powders	31:57	cellulose composite powders	31:57	Selective recrystallization of cellulose composite powders and microstructure creation through 3D binder jetting.
30177161	2	22	with	ink	480:482	arg1	flavour					508:514	flavour	508:514	flavour	508:514	Unfortunately work in this area relevant to food materials is largely unpublished, however a typical application of this technique is sugar powder bound by a water and alcohol based ink with optional colour or flavour demonstrated by commercial ventures.
30177161	2	22	with	ink	480:482	arg1	colour					498:503	optional colour	489:503	optional colour	489:503	Unfortunately work in this area relevant to food materials is largely unpublished, however a typical application of this technique is sugar powder bound by a water and alcohol based ink with optional colour or flavour demonstrated by commercial ventures.
30177161	1	23	theme	Binder	114:119	arg1	technique					158:166	an additive manufacturing technique	132:166	an additive manufacturing technique in which powdered material is sequentially laid down and printed on by an ink binder, in a selective manner, to form a 3D object	132:295	Binder jetting is an additive manufacturing technique in which powdered material is sequentially laid down and printed on by an ink binder, in a selective manner, to form a 3D object.
30177161	1	23	theme	Binder	114:119	arg1	jetting					121:127	Binder jetting	114:127	Binder jetting	114:127	Binder jetting is an additive manufacturing technique in which powdered material is sequentially laid down and printed on by an ink binder, in a selective manner, to form a 3D object.
30177161	2	24	theme	typical	391:397	arg1	application					399:409	a typical application	389:409	a typical application of this technique	389:427	Unfortunately work in this area relevant to food materials is largely unpublished, however a typical application of this technique is sugar powder bound by a water and alcohol based ink with optional colour or flavour demonstrated by commercial ventures.
30177161	2	24	theme	typical	391:397	arg1	powder					438:443	sugar powder	432:443	sugar powder bound by a water and alcohol based ink with optional colour or flavour demonstrated by commercial ventures	432:550	Unfortunately work in this area relevant to food materials is largely unpublished, however a typical application of this technique is sugar powder bound by a water and alcohol based ink with optional colour or flavour demonstrated by commercial ventures.
30177161	4	25	located	present	930:936	arg2	polysaccharides					909:923	interacting polysaccharides	897:923	interacting polysaccharides	897:923	Powders comprising predominantly of ball milled, amorphous cellulose were successfully used to create 3D structures when interacting polysaccharides were present in the ink (xanthan gum) and as a proportion of the powder component (glucomannan) by inducing selective recrystallization.
30177161	4	25	located	present	930:936	arg1	gum					958:960	the ink (xanthan gum)	941:961	the ink (xanthan gum)	941:961	Powders comprising predominantly of ball milled, amorphous cellulose were successfully used to create 3D structures when interacting polysaccharides were present in the ink (xanthan gum) and as a proportion of the powder component (glucomannan) by inducing selective recrystallization.
30177161	3	26	theme	prior	680:684	arg1	powders					672:678	test prototype powders	657:678	test prototype powders prior to producing quantities typically used in commercially available binder jetting machines	657:773	In this work we demonstrate the use of a small scale powder layering device under an ink jet printer to test prototype powders prior to producing quantities typically used in commercially available binder jetting machines.
30177161	3	27	theme	small	594:598	arg1	device					622:627	a small scale powder layering device	592:627	a small scale powder layering device	592:627	In this work we demonstrate the use of a small scale powder layering device under an ink jet printer to test prototype powders prior to producing quantities typically used in commercially available binder jetting machines.
30177161	0	28	theme	powders	51:57	arg1	recrystallization					10:26	Selective recrystallization	0:26	Selective recrystallization of cellulose composite powders and microstructure creation through 3D binder jetting.	0:112	Selective recrystallization of cellulose composite powders and microstructure creation through 3D binder jetting.
30177161	3	29	theme	scale	600:604	arg1	device					622:627	a small scale powder layering device	592:627	a small scale powder layering device	592:627	In this work we demonstrate the use of a small scale powder layering device under an ink jet printer to test prototype powders prior to producing quantities typically used in commercially available binder jetting machines.
30177161	4	30	theme	powder	990:995	arg1	glucomannan					1008:1018	glucomannan	1008:1018	glucomannan	1008:1018	Powders comprising predominantly of ball milled, amorphous cellulose were successfully used to create 3D structures when interacting polysaccharides were present in the ink (xanthan gum) and as a proportion of the powder component (glucomannan) by inducing selective recrystallization.
30177161	4	30	theme	powder	990:995	arg1	component					997:1005	the powder component	986:1005	the powder component (glucomannan)	986:1019	Powders comprising predominantly of ball milled, amorphous cellulose were successfully used to create 3D structures when interacting polysaccharides were present in the ink (xanthan gum) and as a proportion of the powder component (glucomannan) by inducing selective recrystallization.
30177161	0	31	theme	composite	41:49	arg1	powders					51:57	cellulose composite powders	31:57	cellulose composite powders	31:57	Selective recrystallization of cellulose composite powders and microstructure creation through 3D binder jetting.
30177161	4	32	theme	xanthan	950:956	arg1	gum					958:960	the ink (xanthan gum)	941:961	the ink (xanthan gum)	941:961	Powders comprising predominantly of ball milled, amorphous cellulose were successfully used to create 3D structures when interacting polysaccharides were present in the ink (xanthan gum) and as a proportion of the powder component (glucomannan) by inducing selective recrystallization.
30177161	2	33	theme	optional	489:496	arg1	colour					498:503	optional colour	489:503	optional colour	489:503	Unfortunately work in this area relevant to food materials is largely unpublished, however a typical application of this technique is sugar powder bound by a water and alcohol based ink with optional colour or flavour demonstrated by commercial ventures.
30177161	3	34	theme	available	741:749	arg1	machines					766:773	commercially available binder jetting machines	728:773	commercially available binder jetting machines	728:773	In this work we demonstrate the use of a small scale powder layering device under an ink jet printer to test prototype powders prior to producing quantities typically used in commercially available binder jetting machines.
30177161	2	35	theme	sugar	432:436	arg1	application					399:409	a typical application	389:409	a typical application of this technique	389:427	Unfortunately work in this area relevant to food materials is largely unpublished, however a typical application of this technique is sugar powder bound by a water and alcohol based ink with optional colour or flavour demonstrated by commercial ventures.
30177161	2	35	theme	sugar	432:436	arg1	powder					438:443	sugar powder	432:443	sugar powder bound by a water and alcohol based ink with optional colour or flavour demonstrated by commercial ventures	432:550	Unfortunately work in this area relevant to food materials is largely unpublished, however a typical application of this technique is sugar powder bound by a water and alcohol based ink with optional colour or flavour demonstrated by commercial ventures.
30177161	1	36	theme	3D	287:288	arg1	object					290:295	a 3D object	285:295	a 3D object	285:295	Binder jetting is an additive manufacturing technique in which powdered material is sequentially laid down and printed on by an ink binder, in a selective manner, to form a 3D object.
30177161	0	37	theme	creation	78:85	arg1	recrystallization					10:26	Selective recrystallization	0:26	Selective recrystallization of cellulose composite powders and microstructure creation through 3D binder jetting.	0:112	Selective recrystallization of cellulose composite powders and microstructure creation through 3D binder jetting.
30177161	4	38	theme	interacting	897:907	arg1	polysaccharides					909:923	interacting polysaccharides	897:923	interacting polysaccharides	897:923	Powders comprising predominantly of ball milled, amorphous cellulose were successfully used to create 3D structures when interacting polysaccharides were present in the ink (xanthan gum) and as a proportion of the powder component (glucomannan) by inducing selective recrystallization.
30177161	5	39	theme	such	1119:1122	arg1	formulations					1124:1135	such formulations	1119:1135	such formulations	1119:1135	These ingredients are categorized as dietary fibre, thus such formulations can be used to create low-calorie 3D printed food designs to be used within food products.
30177161	2	40	theme	commercial	532:541	arg1	ventures					543:550	commercial ventures	532:550	commercial ventures	532:550	Unfortunately work in this area relevant to food materials is largely unpublished, however a typical application of this technique is sugar powder bound by a water and alcohol based ink with optional colour or flavour demonstrated by commercial ventures.
30177161	3	41	theme	device	622:627	arg1	use					585:587	the use	581:587	the use of a small scale powder layering device	581:627	In this work we demonstrate the use of a small scale powder layering device under an ink jet printer to test prototype powders prior to producing quantities typically used in commercially available binder jetting machines.
30177161	4	42	theme	ink	945:947	arg1	gum					958:960	the ink (xanthan gum)	941:961	the ink (xanthan gum)	941:961	Powders comprising predominantly of ball milled, amorphous cellulose were successfully used to create 3D structures when interacting polysaccharides were present in the ink (xanthan gum) and as a proportion of the powder component (glucomannan) by inducing selective recrystallization.
30177161	4	43	theme	3D	878:879	arg1	structures					881:890	3D structures	878:890	3D structures	878:890	Powders comprising predominantly of ball milled, amorphous cellulose were successfully used to create 3D structures when interacting polysaccharides were present in the ink (xanthan gum) and as a proportion of the powder component (glucomannan) by inducing selective recrystallization.
30177161	5	44	used	used	1144:1147	arg2	formulations					1124:1135	such formulations	1119:1135	such formulations	1119:1135	These ingredients are categorized as dietary fibre, thus such formulations can be used to create low-calorie 3D printed food designs to be used within food products.
30177161	4	45	from	present	930:936	arg1	gum					958:960	the ink (xanthan gum)	941:961	the ink (xanthan gum)	941:961	Powders comprising predominantly of ball milled, amorphous cellulose were successfully used to create 3D structures when interacting polysaccharides were present in the ink (xanthan gum) and as a proportion of the powder component (glucomannan) by inducing selective recrystallization.
30177161	5	46	theme	printed	1174:1180	arg1	designs					1187:1193	low-calorie 3D printed food designs	1159:1193	low-calorie 3D printed food designs	1159:1193	These ingredients are categorized as dietary fibre, thus such formulations can be used to create low-calorie 3D printed food designs to be used within food products.
30177161	1	47	theme	additive	135:142	arg1	technique					158:166	an additive manufacturing technique	132:166	an additive manufacturing technique in which powdered material is sequentially laid down and printed on by an ink binder, in a selective manner, to form a 3D object	132:295	Binder jetting is an additive manufacturing technique in which powdered material is sequentially laid down and printed on by an ink binder, in a selective manner, to form a 3D object.
30177161	1	47	theme	additive	135:142	arg1	jetting					121:127	Binder jetting	114:127	Binder jetting	114:127	Binder jetting is an additive manufacturing technique in which powdered material is sequentially laid down and printed on by an ink binder, in a selective manner, to form a 3D object.
30177161	3	48	theme	jetting	758:764	arg1	machines					766:773	commercially available binder jetting machines	728:773	commercially available binder jetting machines	728:773	In this work we demonstrate the use of a small scale powder layering device under an ink jet printer to test prototype powders prior to producing quantities typically used in commercially available binder jetting machines.
30177161	3	49	theme	powder	606:611	arg1	device					622:627	a small scale powder layering device	592:627	a small scale powder layering device	592:627	In this work we demonstrate the use of a small scale powder layering device under an ink jet printer to test prototype powders prior to producing quantities typically used in commercially available binder jetting machines.
30177161	1	50	theme	manufacturing	144:156	arg1	technique					158:166	an additive manufacturing technique	132:166	an additive manufacturing technique in which powdered material is sequentially laid down and printed on by an ink binder, in a selective manner, to form a 3D object	132:295	Binder jetting is an additive manufacturing technique in which powdered material is sequentially laid down and printed on by an ink binder, in a selective manner, to form a 3D object.
30177161	1	50	theme	manufacturing	144:156	arg1	jetting					121:127	Binder jetting	114:127	Binder jetting	114:127	Binder jetting is an additive manufacturing technique in which powdered material is sequentially laid down and printed on by an ink binder, in a selective manner, to form a 3D object.
30177161	3	51	theme	layering	613:620	arg1	device					622:627	a small scale powder layering device	592:627	a small scale powder layering device	592:627	In this work we demonstrate the use of a small scale powder layering device under an ink jet printer to test prototype powders prior to producing quantities typically used in commercially available binder jetting machines.
30177161	4	52	attach	present	930:936	arg2	polysaccharides					909:923	interacting polysaccharides	897:923	interacting polysaccharides	897:923	Powders comprising predominantly of ball milled, amorphous cellulose were successfully used to create 3D structures when interacting polysaccharides were present in the ink (xanthan gum) and as a proportion of the powder component (glucomannan) by inducing selective recrystallization.
30177161	4	52	attach	present	930:936	arg1	gum					958:960	the ink (xanthan gum)	941:961	the ink (xanthan gum)	941:961	Powders comprising predominantly of ball milled, amorphous cellulose were successfully used to create 3D structures when interacting polysaccharides were present in the ink (xanthan gum) and as a proportion of the powder component (glucomannan) by inducing selective recrystallization.
30177161	2	53	theme	food	342:345	arg1	materials					347:355	food materials	342:355	food materials	342:355	Unfortunately work in this area relevant to food materials is largely unpublished, however a typical application of this technique is sugar powder bound by a water and alcohol based ink with optional colour or flavour demonstrated by commercial ventures.
30177161	4	54	theme	selective	1033:1041	arg1	recrystallization					1043:1059	selective recrystallization	1033:1059	selective recrystallization	1033:1059	Powders comprising predominantly of ball milled, amorphous cellulose were successfully used to create 3D structures when interacting polysaccharides were present in the ink (xanthan gum) and as a proportion of the powder component (glucomannan) by inducing selective recrystallization.
30177161	1	55	theme	ink	242:244	arg1	binder					246:251	an ink binder	239:251	an ink binder	239:251	Binder jetting is an additive manufacturing technique in which powdered material is sequentially laid down and printed on by an ink binder, in a selective manner, to form a 3D object.
30177161	0	56	theme	microstructure	63:76	arg1	creation					78:85	microstructure creation	63:85	microstructure creation	63:85	Selective recrystallization of cellulose composite powders and microstructure creation through 3D binder jetting.
30177161	3	57	theme	jet	642:644	arg1	printer					646:652	an ink jet printer	635:652	an ink jet printer to test prototype powders prior to producing quantities typically used in commercially available binder jetting machines	635:773	In this work we demonstrate the use of a small scale powder layering device under an ink jet printer to test prototype powders prior to producing quantities typically used in commercially available binder jetting machines.
30177161	5	58	theme	3D	1171:1172	arg1	designs					1187:1193	low-calorie 3D printed food designs	1159:1193	low-calorie 3D printed food designs	1159:1193	These ingredients are categorized as dietary fibre, thus such formulations can be used to create low-calorie 3D printed food designs to be used within food products.
30177161	4	59	from	gum	958:960	arg1	present					930:936	present	930:936	present	930:936	Powders comprising predominantly of ball milled, amorphous cellulose were successfully used to create 3D structures when interacting polysaccharides were present in the ink (xanthan gum) and as a proportion of the powder component (glucomannan) by inducing selective recrystallization.
30177161	3	60	theme	binder	751:756	arg1	machines					766:773	commercially available binder jetting machines	728:773	commercially available binder jetting machines	728:773	In this work we demonstrate the use of a small scale powder layering device under an ink jet printer to test prototype powders prior to producing quantities typically used in commercially available binder jetting machines.
31659686	6	0	theme	rRNA	760:763	arg1	16S					776:778	six 16S	772:778	six 16S	772:778	The genome size and genomic G + C content of strain NKC3-5T were 3,754,174 bp and 45.9 mol%, respectively; it contained 3,630 coding sequences, 16S rRNA genes (six 16S, five 5S, and five 23S), and 59 tRNA genes.
31659686	6	0	theme	rRNA	760:763	arg1	5S					786:787	five 5S	781:787	five 5S	781:787	The genome size and genomic G + C content of strain NKC3-5T were 3,754,174 bp and 45.9 mol%, respectively; it contained 3,630 coding sequences, 16S rRNA genes (six 16S, five 5S, and five 23S), and 59 tRNA genes.
31659686	6	0	theme	rRNA	760:763	arg1	23S					799:801	five 23S	794:801	five 23S	794:801	The genome size and genomic G + C content of strain NKC3-5T were 3,754,174 bp and 45.9 mol%, respectively; it contained 3,630 coding sequences, 16S rRNA genes (six 16S, five 5S, and five 23S), and 59 tRNA genes.
31659686	6	0	theme	rRNA	760:763	arg1	genes					765:769	16S rRNA genes	756:769	16S rRNA genes (six 16S, five 5S, and five 23S)	756:802	The genome size and genomic G + C content of strain NKC3-5T were 3,754,174 bp and 45.9 mol%, respectively; it contained 3,630 coding sequences, 16S rRNA genes (six 16S, five 5S, and five 23S), and 59 tRNA genes.
31659686	10	1	contain	had	1442:1444	arg2	POGs					1481:1484	POGs	1481:1484	POGs	1481:1484	Comparative genomic analysis revealed that strain NKC3-5T had 716 pan-genome orthologous groups (POGs), dominated with carbohydrate metabolism.
31659686	10	1	contain	had	1442:1444	arg2	groups					1473:1478	716 pan-genome orthologous groups	1446:1478	716 pan-genome orthologous groups (POGs)	1446:1485	Comparative genomic analysis revealed that strain NKC3-5T had 716 pan-genome orthologous groups (POGs), dominated with carbohydrate metabolism.
31659686	10	1	contain	had	1442:1444	arg1	NKC3-5T					1434:1440	strain NKC3-5T	1427:1440	strain NKC3-5T	1427:1440	Comparative genomic analysis revealed that strain NKC3-5T had 716 pan-genome orthologous groups (POGs), dominated with carbohydrate metabolism.
31659686	7	2	theme	published	1021:1029	arg1	species					1031:1037	other published species	1015:1037	other published species	1015:1037	Phylogenetic analysis based on 16S rRNA showed that strain NKC3-5T clustered with bacterium Salicibibacter kimchii NKC1-1T, with a similarity of 96.2-97.6%, but formed a distinct branch with other published species of the family Bacillaceae.
31659686	7	3	theme	kimchii	931:937	arg1	NKC1-1T					939:945	bacterium Salicibibacter kimchii NKC1-1T	906:945	bacterium Salicibibacter kimchii NKC1-1T	906:945	Phylogenetic analysis based on 16S rRNA showed that strain NKC3-5T clustered with bacterium Salicibibacter kimchii NKC1-1T, with a similarity of 96.2-97.6%, but formed a distinct branch with other published species of the family Bacillaceae.
31659686	4	4	dep	NaCl	415:418	arg1	%					407:407	%	407:407	%	407:407	The strain NKC3-5T could grow at up to 20.0% (w/v) NaCl (optimum 10%), pH 6.5-10.0 (optimum pH 9.0), and 25-40°C (optimum 35°C).
31659686	16	5	theme	KACC	2287:2290	arg1	33437T					2305:2310	= KACC 21230T = JCM 33437T	2285:2310	= KACC 21230T = JCM 33437T	2285:2310	nov. is proposed, with the type strain NKC3-5T (= KACC 21230T = JCM 33437T).
31659686	16	5	theme	KACC	2287:2290	arg1	NKC3-5T					2276:2282	the type strain NKC3-5T	2260:2282	the type strain NKC3-5T (= KACC 21230T = JCM 33437T)	2260:2311	nov. is proposed, with the type strain NKC3-5T (= KACC 21230T = JCM 33437T).
31659686	6	6	dep	genes	765:769	arg1	16S					776:778	six 16S	772:778	six 16S	772:778	The genome size and genomic G + C content of strain NKC3-5T were 3,754,174 bp and 45.9 mol%, respectively; it contained 3,630 coding sequences, 16S rRNA genes (six 16S, five 5S, and five 23S), and 59 tRNA genes.
31659686	6	6	dep	genes	765:769	arg1	5S					786:787	five 5S	781:787	five 5S	781:787	The genome size and genomic G + C content of strain NKC3-5T were 3,754,174 bp and 45.9 mol%, respectively; it contained 3,630 coding sequences, 16S rRNA genes (six 16S, five 5S, and five 23S), and 59 tRNA genes.
31659686	6	6	dep	genes	765:769	arg1	23S					799:801	five 23S	794:801	five 23S	794:801	The genome size and genomic G + C content of strain NKC3-5T were 3,754,174 bp and 45.9 mol%, respectively; it contained 3,630 coding sequences, 16S rRNA genes (six 16S, five 5S, and five 23S), and 59 tRNA genes.
31659686	6	6	dep	genes	765:769	arg1	genes					765:769	16S rRNA genes	756:769	16S rRNA genes (six 16S, five 5S, and five 23S)	756:802	The genome size and genomic G + C content of strain NKC3-5T were 3,754,174 bp and 45.9 mol%, respectively; it contained 3,630 coding sequences, 16S rRNA genes (six 16S, five 5S, and five 23S), and 59 tRNA genes.
31659686	7	7	theme	bacterium	906:914	arg1	NKC1-1T					939:945	bacterium Salicibibacter kimchii NKC1-1T	906:945	bacterium Salicibibacter kimchii NKC1-1T	906:945	Phylogenetic analysis based on 16S rRNA showed that strain NKC3-5T clustered with bacterium Salicibibacter kimchii NKC1-1T, with a similarity of 96.2-97.6%, but formed a distinct branch with other published species of the family Bacillaceae.
31659686	13	8	theme	major	1810:1814	arg1	anteiso-C17:0					1772:1784	anteiso-C17:0	1772:1784	anteiso-C17:0	1772:1784	Anteiso-C15:0, iso-C17:0, anteiso-C17:0, and iso-C15:0 were the major cellular fatty acids, and menaquinone-7 was the major isoprenoid quinone present in strain NKC3-5T.
31659686	13	8	theme	major	1810:1814	arg1	iso-C15:0					1791:1799	iso-C15:0	1791:1799	iso-C15:0	1791:1799	Anteiso-C15:0, iso-C17:0, anteiso-C17:0, and iso-C15:0 were the major cellular fatty acids, and menaquinone-7 was the major isoprenoid quinone present in strain NKC3-5T.
31659686	13	8	theme	major	1810:1814	arg1	iso-C17:0					1761:1769	iso-C17:0	1761:1769	iso-C17:0	1761:1769	Anteiso-C15:0, iso-C17:0, anteiso-C17:0, and iso-C15:0 were the major cellular fatty acids, and menaquinone-7 was the major isoprenoid quinone present in strain NKC3-5T.
31659686	13	8	theme	major	1810:1814	arg1	acids					1831:1835	the major cellular fatty acids	1806:1835	the major cellular fatty acids	1806:1835	Anteiso-C15:0, iso-C17:0, anteiso-C17:0, and iso-C15:0 were the major cellular fatty acids, and menaquinone-7 was the major isoprenoid quinone present in strain NKC3-5T.
31659686	13	8	theme	major	1810:1814	arg1	Anteiso-C15:0					1746:1758	Anteiso-C15:0	1746:1758	Anteiso-C15:0	1746:1758	Anteiso-C15:0, iso-C17:0, anteiso-C17:0, and iso-C15:0 were the major cellular fatty acids, and menaquinone-7 was the major isoprenoid quinone present in strain NKC3-5T.
31659686	6	9	theme	coding	738:743	arg1	sequences					745:753	3,630 coding sequences	732:753	3,630 coding sequences	732:753	The genome size and genomic G + C content of strain NKC3-5T were 3,754,174 bp and 45.9 mol%, respectively; it contained 3,630 coding sequences, 16S rRNA genes (six 16S, five 5S, and five 23S), and 59 tRNA genes.
31659686	14	10	theme	meso-diaminopimelic	1979:1997	arg1	acid					2031:2034	the diagnostic diamino acid	2008:2034	the diagnostic diamino acid	2008:2034	Cell wall peptidoglycan analysis of strain NKC3-5T showed that meso-diaminopimelic acid was the diagnostic diamino acid.
31659686	14	10	theme	meso-diaminopimelic	1979:1997	arg1	acid					1999:2002	meso-diaminopimelic acid	1979:2002	meso-diaminopimelic acid	1979:2002	Cell wall peptidoglycan analysis of strain NKC3-5T showed that meso-diaminopimelic acid was the diagnostic diamino acid.
31659686	6	11	theme	strain	657:662	arg1	NKC3-5T					664:670	strain NKC3-5T	657:670	strain NKC3-5T	657:670	The genome size and genomic G + C content of strain NKC3-5T were 3,754,174 bp and 45.9 mol%, respectively; it contained 3,630 coding sequences, 16S rRNA genes (six 16S, five 5S, and five 23S), and 59 tRNA genes.
31659686	7	12	theme	%	978:978	arg1	similarity					955:964	a similarity	953:964	a similarity of 96.2-97.6%	953:978	Phylogenetic analysis based on 16S rRNA showed that strain NKC3-5T clustered with bacterium Salicibibacter kimchii NKC1-1T, with a similarity of 96.2-97.6%, but formed a distinct branch with other published species of the family Bacillaceae.
31659686	10	13	theme	genomic	1396:1402	arg1	analysis					1404:1411	Comparative genomic analysis	1384:1411	Comparative genomic analysis	1384:1411	Comparative genomic analysis revealed that strain NKC3-5T had 716 pan-genome orthologous groups (POGs), dominated with carbohydrate metabolism.
31659686	4	14	theme	strain	368:373	arg1	NKC3-5T					375:381	The strain NKC3-5T	364:381	The strain NKC3-5T	364:381	The strain NKC3-5T could grow at up to 20.0% (w/v) NaCl (optimum 10%), pH 6.5-10.0 (optimum pH 9.0), and 25-40°C (optimum 35°C).
31659686	6	15	contain	contained	722:730	arg2	genes					817:821	59 tRNA genes	809:821	59 tRNA genes	809:821	The genome size and genomic G + C content of strain NKC3-5T were 3,754,174 bp and 45.9 mol%, respectively; it contained 3,630 coding sequences, 16S rRNA genes (six 16S, five 5S, and five 23S), and 59 tRNA genes.
31659686	6	15	contain	contained	722:730	arg2	sequences					745:753	3,630 coding sequences	732:753	3,630 coding sequences	732:753	The genome size and genomic G + C content of strain NKC3-5T were 3,754,174 bp and 45.9 mol%, respectively; it contained 3,630 coding sequences, 16S rRNA genes (six 16S, five 5S, and five 23S), and 59 tRNA genes.
31659686	6	15	contain	contained	722:730	arg2	23S					799:801	five 23S	794:801	five 23S	794:801	The genome size and genomic G + C content of strain NKC3-5T were 3,754,174 bp and 45.9 mol%, respectively; it contained 3,630 coding sequences, 16S rRNA genes (six 16S, five 5S, and five 23S), and 59 tRNA genes.
31659686	6	15	contain	contained	722:730	arg2	16S					776:778	six 16S	772:778	six 16S	772:778	The genome size and genomic G + C content of strain NKC3-5T were 3,754,174 bp and 45.9 mol%, respectively; it contained 3,630 coding sequences, 16S rRNA genes (six 16S, five 5S, and five 23S), and 59 tRNA genes.
31659686	6	15	contain	contained	722:730	arg2	genes					765:769	16S rRNA genes	756:769	16S rRNA genes (six 16S, five 5S, and five 23S)	756:802	The genome size and genomic G + C content of strain NKC3-5T were 3,754,174 bp and 45.9 mol%, respectively; it contained 3,630 coding sequences, 16S rRNA genes (six 16S, five 5S, and five 23S), and 59 tRNA genes.
31659686	6	15	contain	contained	722:730	arg2	5S					786:787	five 5S	781:787	five 5S	781:787	The genome size and genomic G + C content of strain NKC3-5T were 3,754,174 bp and 45.9 mol%, respectively; it contained 3,630 coding sequences, 16S rRNA genes (six 16S, five 5S, and five 23S), and 59 tRNA genes.
31659686	6	15	contain	contained	722:730	arg1	it					719:720	it	719:720	it	719:720	The genome size and genomic G + C content of strain NKC3-5T were 3,754,174 bp and 45.9 mol%, respectively; it contained 3,630 coding sequences, 16S rRNA genes (six 16S, five 5S, and five 23S), and 59 tRNA genes.
31659686	14	16	theme	diagnostic	2012:2021	arg1	acid					2031:2034	the diagnostic diamino acid	2008:2034	the diagnostic diamino acid	2008:2034	Cell wall peptidoglycan analysis of strain NKC3-5T showed that meso-diaminopimelic acid was the diagnostic diamino acid.
31659686	14	16	theme	diagnostic	2012:2021	arg1	acid					1999:2002	meso-diaminopimelic acid	1979:2002	meso-diaminopimelic acid	1979:2002	Cell wall peptidoglycan analysis of strain NKC3-5T showed that meso-diaminopimelic acid was the diagnostic diamino acid.
31659686	11	17	theme	strain	1600:1605	arg1	NKC3-5T					1607:1613	strain NKC3-5T	1600:1613	strain NKC3-5T	1600:1613	Phylogenomic analysis based on the concatenated core POGs revealed that strain NKC3-5T was closely related to Salicibibacter kimchii.
31659686	9	18	theme	carbohydrate	1264:1275	arg1	genes					1321:1325	carbohydrate, amino acid, and vitamin metabolism related genes	1264:1325	carbohydrate, amino acid, and vitamin metabolism related genes	1264:1325	Using functional genome annotation, the result found that carbohydrate, amino acid, and vitamin metabolism related genes were highly distributed in the genome of strain NKC3-5T.
31659686	10	19	theme	strain	1427:1432	arg1	NKC3-5T					1434:1440	strain NKC3-5T	1427:1440	strain NKC3-5T	1427:1440	Comparative genomic analysis revealed that strain NKC3-5T had 716 pan-genome orthologous groups (POGs), dominated with carbohydrate metabolism.
31659686	11	20	theme	Salicibibacter	1638:1651	arg1	kimchii					1653:1659	Salicibibacter kimchii	1638:1659	Salicibibacter kimchii	1638:1659	Phylogenomic analysis based on the concatenated core POGs revealed that strain NKC3-5T was closely related to Salicibibacter kimchii.
31659686	7	21	theme	16S	855:857	arg1	rRNA					859:862	16S rRNA	855:862	16S rRNA	855:862	Phylogenetic analysis based on 16S rRNA showed that strain NKC3-5T clustered with bacterium Salicibibacter kimchii NKC1-1T, with a similarity of 96.2-97.6%, but formed a distinct branch with other published species of the family Bacillaceae.
31659686	15	22	theme	halophilus	2222:2231	arg1	sp					2233:2234	the name Salicibibacter halophilus sp	2198:2234	the name Salicibibacter halophilus sp	2198:2234	The phephenotypic, genomic, phylogenetic, and chemotaxonomic properties reveal that the strain represents a novel species of the genus Salicibibacter, for which the name Salicibibacter halophilus sp.
31659686	9	23	theme	amino	1278:1282	arg1	acid					1284:1287	amino acid	1278:1287	amino acid	1278:1287	Using functional genome annotation, the result found that carbohydrate, amino acid, and vitamin metabolism related genes were highly distributed in the genome of strain NKC3-5T.
31659686	3	24	theme	strain	307:312	arg1	NKC3-5T					314:320	isolated strain NKC3-5T	298:320	isolated strain NKC3-5T	298:320	Cells of isolated strain NKC3-5T were 0.5-0.7 μm wide and 1.4-2.8 μm long.
31659686	13	25	from	NKC3-5T	1907:1913	arg1	present					1889:1895	present	1889:1895	present	1889:1895	Anteiso-C15:0, iso-C17:0, anteiso-C17:0, and iso-C15:0 were the major cellular fatty acids, and menaquinone-7 was the major isoprenoid quinone present in strain NKC3-5T.
31659686	15	26	theme	name	2202:2205	arg1	sp					2233:2234	the name Salicibibacter halophilus sp	2198:2234	the name Salicibibacter halophilus sp	2198:2234	The phephenotypic, genomic, phylogenetic, and chemotaxonomic properties reveal that the strain represents a novel species of the genus Salicibibacter, for which the name Salicibibacter halophilus sp.
31659686	7	27	theme	Phylogenetic	824:835	arg1	analysis					837:844	Phylogenetic analysis	824:844	Phylogenetic analysis based on 16S rRNA	824:862	Phylogenetic analysis based on 16S rRNA showed that strain NKC3-5T clustered with bacterium Salicibibacter kimchii NKC1-1T, with a similarity of 96.2-97.6%, but formed a distinct branch with other published species of the family Bacillaceae.
31659686	9	28	theme	functional	1212:1221	arg1	annotation					1230:1239	functional genome annotation	1212:1239	functional genome annotation	1212:1239	Using functional genome annotation, the result found that carbohydrate, amino acid, and vitamin metabolism related genes were highly distributed in the genome of strain NKC3-5T.
31659686	16	29	theme	21230T	2292:2297	arg1	33437T					2305:2310	= KACC 21230T = JCM 33437T	2285:2310	= KACC 21230T = JCM 33437T	2285:2310	nov. is proposed, with the type strain NKC3-5T (= KACC 21230T = JCM 33437T).
31659686	16	29	theme	21230T	2292:2297	arg1	NKC3-5T					2276:2282	the type strain NKC3-5T	2260:2282	the type strain NKC3-5T (= KACC 21230T = JCM 33437T)	2260:2311	nov. is proposed, with the type strain NKC3-5T (= KACC 21230T = JCM 33437T).
31659686	9	30	theme	vitamin	1294:1300	arg1	metabolism					1302:1311	vitamin metabolism	1294:1311	vitamin metabolism	1294:1311	Using functional genome annotation, the result found that carbohydrate, amino acid, and vitamin metabolism related genes were highly distributed in the genome of strain NKC3-5T.
31659686	6	31	theme	tRNA	812:815	arg1	genes					817:821	59 tRNA genes	809:821	59 tRNA genes	809:821	The genome size and genomic G + C content of strain NKC3-5T were 3,754,174 bp and 45.9 mol%, respectively; it contained 3,630 coding sequences, 16S rRNA genes (six 16S, five 5S, and five 23S), and 59 tRNA genes.
31659686	8	32	theme	strain	1102:1107	arg1	NKC3-5T					1109:1115	strain NKC3-5T	1102:1115	strain NKC3-5T	1102:1115	In addition, OrthoANI value between strain NKC3-5T and Salicibibacter kimchii NKC1-1T was far lower than the species demarcation threshold.
31659686	7	33	theme	other	1015:1019	arg1	species					1031:1037	other published species	1015:1037	other published species	1015:1037	Phylogenetic analysis based on 16S rRNA showed that strain NKC3-5T clustered with bacterium Salicibibacter kimchii NKC1-1T, with a similarity of 96.2-97.6%, but formed a distinct branch with other published species of the family Bacillaceae.
31659686	13	34	theme	strain	1900:1905	arg1	NKC3-5T					1907:1913	strain NKC3-5T	1900:1913	strain NKC3-5T	1900:1913	Anteiso-C15:0, iso-C17:0, anteiso-C17:0, and iso-C15:0 were the major cellular fatty acids, and menaquinone-7 was the major isoprenoid quinone present in strain NKC3-5T.
31659686	9	35	theme	related	1313:1319	arg1	genes					1321:1325	carbohydrate, amino acid, and vitamin metabolism related genes	1264:1325	carbohydrate, amino acid, and vitamin metabolism related genes	1264:1325	Using functional genome annotation, the result found that carbohydrate, amino acid, and vitamin metabolism related genes were highly distributed in the genome of strain NKC3-5T.
31659686	15	36	theme	phephenotypic	2041:2053	arg1	properties					2098:2107	The phephenotypic, genomic, phylogenetic, and chemotaxonomic properties	2037:2107	The phephenotypic, genomic, phylogenetic, and chemotaxonomic properties	2037:2107	The phephenotypic, genomic, phylogenetic, and chemotaxonomic properties reveal that the strain represents a novel species of the genus Salicibibacter, for which the name Salicibibacter halophilus sp.
31659686	15	37	theme	genomic	2056:2062	arg1	properties					2098:2107	The phephenotypic, genomic, phylogenetic, and chemotaxonomic properties	2037:2107	The phephenotypic, genomic, phylogenetic, and chemotaxonomic properties	2037:2107	The phephenotypic, genomic, phylogenetic, and chemotaxonomic properties reveal that the strain represents a novel species of the genus Salicibibacter, for which the name Salicibibacter halophilus sp.
31659686	14	38	theme	NKC3-5T	1959:1965	arg1	analysis					1940:1947	Cell wall peptidoglycan analysis	1916:1947	Cell wall peptidoglycan analysis of strain NKC3-5T	1916:1965	Cell wall peptidoglycan analysis of strain NKC3-5T showed that meso-diaminopimelic acid was the diagnostic diamino acid.
31659686	8	39	theme	kimchii	1136:1142	arg1	NKC1-1T					1144:1150	Salicibibacter kimchii NKC1-1T	1121:1150	Salicibibacter kimchii NKC1-1T	1121:1150	In addition, OrthoANI value between strain NKC3-5T and Salicibibacter kimchii NKC1-1T was far lower than the species demarcation threshold.
31659686	11	40	theme	core	1576:1579	arg1	POGs					1581:1584	the concatenated core POGs	1559:1584	the concatenated core POGs	1559:1584	Phylogenomic analysis based on the concatenated core POGs revealed that strain NKC3-5T was closely related to Salicibibacter kimchii.
31659686	13	41	theme	isoprenoid	1870:1879	arg1	quinone					1881:1887	the major isoprenoid quinone	1860:1887	the major isoprenoid quinone present in strain NKC3-5T	1860:1913	Anteiso-C15:0, iso-C17:0, anteiso-C17:0, and iso-C15:0 were the major cellular fatty acids, and menaquinone-7 was the major isoprenoid quinone present in strain NKC3-5T.
31659686	13	41	theme	isoprenoid	1870:1879	arg1	menaquinone-7					1842:1854	menaquinone-7	1842:1854	menaquinone-7	1842:1854	Anteiso-C15:0, iso-C17:0, anteiso-C17:0, and iso-C15:0 were the major cellular fatty acids, and menaquinone-7 was the major isoprenoid quinone present in strain NKC3-5T.
31659686	15	42	theme	phylogenetic	2065:2076	arg1	properties					2098:2107	The phephenotypic, genomic, phylogenetic, and chemotaxonomic properties	2037:2107	The phephenotypic, genomic, phylogenetic, and chemotaxonomic properties	2037:2107	The phephenotypic, genomic, phylogenetic, and chemotaxonomic properties reveal that the strain represents a novel species of the genus Salicibibacter, for which the name Salicibibacter halophilus sp.
31659686	4	43	theme	optimum	421:427	arg1	%					431:431	optimum 10%	421:431	optimum 10%	421:431	The strain NKC3-5T could grow at up to 20.0% (w/v) NaCl (optimum 10%), pH 6.5-10.0 (optimum pH 9.0), and 25-40°C (optimum 35°C).
31659686	4	43	theme	optimum	421:427	arg1	NaCl					415:418	up to 20.0% (w/v) NaCl	397:418	up to 20.0% (w/v) NaCl (optimum 10%)	397:432	The strain NKC3-5T could grow at up to 20.0% (w/v) NaCl (optimum 10%), pH 6.5-10.0 (optimum pH 9.0), and 25-40°C (optimum 35°C).
31659686	10	44	theme	orthologous	1461:1471	arg1	POGs					1481:1484	POGs	1481:1484	POGs	1481:1484	Comparative genomic analysis revealed that strain NKC3-5T had 716 pan-genome orthologous groups (POGs), dominated with carbohydrate metabolism.
31659686	10	44	theme	orthologous	1461:1471	arg1	groups					1473:1478	716 pan-genome orthologous groups	1446:1478	716 pan-genome orthologous groups (POGs)	1446:1485	Comparative genomic analysis revealed that strain NKC3-5T had 716 pan-genome orthologous groups (POGs), dominated with carbohydrate metabolism.
31659686	13	45	theme	present	1889:1895	arg1	quinone					1881:1887	the major isoprenoid quinone	1860:1887	the major isoprenoid quinone present in strain NKC3-5T	1860:1913	Anteiso-C15:0, iso-C17:0, anteiso-C17:0, and iso-C15:0 were the major cellular fatty acids, and menaquinone-7 was the major isoprenoid quinone present in strain NKC3-5T.
31659686	13	45	theme	present	1889:1895	arg1	menaquinone-7					1842:1854	menaquinone-7	1842:1854	menaquinone-7	1842:1854	Anteiso-C15:0, iso-C17:0, anteiso-C17:0, and iso-C15:0 were the major cellular fatty acids, and menaquinone-7 was the major isoprenoid quinone present in strain NKC3-5T.
31659686	14	46	theme	peptidoglycan	1926:1938	arg1	analysis					1940:1947	Cell wall peptidoglycan analysis	1916:1947	Cell wall peptidoglycan analysis of strain NKC3-5T	1916:1965	Cell wall peptidoglycan analysis of strain NKC3-5T showed that meso-diaminopimelic acid was the diagnostic diamino acid.
31659686	16	47	theme	=	2299:2299	arg1	33437T					2305:2310	= KACC 21230T = JCM 33437T	2285:2310	= KACC 21230T = JCM 33437T	2285:2310	nov. is proposed, with the type strain NKC3-5T (= KACC 21230T = JCM 33437T).
31659686	16	47	theme	=	2299:2299	arg1	NKC3-5T					2276:2282	the type strain NKC3-5T	2260:2282	the type strain NKC3-5T (= KACC 21230T = JCM 33437T)	2260:2311	nov. is proposed, with the type strain NKC3-5T (= KACC 21230T = JCM 33437T).
31659686	0	48	theme	Salicibibacter	0:13	arg1	sp					26:27	Salicibibacter halophilus sp	0:27	Salicibibacter halophilus sp.	0:28	Salicibibacter halophilus sp.
31659686	14	49	theme	Cell	1916:1919	arg1	analysis					1940:1947	Cell wall peptidoglycan analysis	1916:1947	Cell wall peptidoglycan analysis of strain NKC3-5T	1916:1965	Cell wall peptidoglycan analysis of strain NKC3-5T showed that meso-diaminopimelic acid was the diagnostic diamino acid.
31659686	6	50	theme	NKC3-5T	664:670	arg1	size					623:626	genome size	616:626	genome size	616:626	The genome size and genomic G + C content of strain NKC3-5T were 3,754,174 bp and 45.9 mol%, respectively; it contained 3,630 coding sequences, 16S rRNA genes (six 16S, five 5S, and five 23S), and 59 tRNA genes.
31659686	6	50	theme	NKC3-5T	664:670	arg1	G					640:640	genomic G + C content	632:652	G	640:640	The genome size and genomic G + C content of strain NKC3-5T were 3,754,174 bp and 45.9 mol%, respectively; it contained 3,630 coding sequences, 16S rRNA genes (six 16S, five 5S, and five 23S), and 59 tRNA genes.
31659686	6	50	theme	NKC3-5T	664:670	arg1	content					646:652	genomic G + C content	632:652	content	646:652	The genome size and genomic G + C content of strain NKC3-5T were 3,754,174 bp and 45.9 mol%, respectively; it contained 3,630 coding sequences, 16S rRNA genes (six 16S, five 5S, and five 23S), and 59 tRNA genes.
31659686	9	51	theme	NKC3-5T	1375:1381	arg1	genome					1358:1363	the genome	1354:1363	the genome of strain NKC3-5T	1354:1381	Using functional genome annotation, the result found that carbohydrate, amino acid, and vitamin metabolism related genes were highly distributed in the genome of strain NKC3-5T.
31659686	8	52	theme	demarcation	1183:1193	arg1	threshold					1195:1203	the species demarcation threshold	1171:1203	the species demarcation threshold	1171:1203	In addition, OrthoANI value between strain NKC3-5T and Salicibibacter kimchii NKC1-1T was far lower than the species demarcation threshold.
31659686	13	53	attach	present	1889:1895	arg1	NKC3-5T					1907:1913	strain NKC3-5T	1900:1913	strain NKC3-5T	1900:1913	Anteiso-C15:0, iso-C17:0, anteiso-C17:0, and iso-C15:0 were the major cellular fatty acids, and menaquinone-7 was the major isoprenoid quinone present in strain NKC3-5T.
31659686	13	53	attach	present	1889:1895	arg2	menaquinone-7					1842:1854	menaquinone-7	1842:1854	menaquinone-7	1842:1854	Anteiso-C15:0, iso-C17:0, anteiso-C17:0, and iso-C15:0 were the major cellular fatty acids, and menaquinone-7 was the major isoprenoid quinone present in strain NKC3-5T.
31659686	13	53	attach	present	1889:1895	arg2	quinone					1881:1887	the major isoprenoid quinone	1860:1887	the major isoprenoid quinone present in strain NKC3-5T	1860:1913	Anteiso-C15:0, iso-C17:0, anteiso-C17:0, and iso-C15:0 were the major cellular fatty acids, and menaquinone-7 was the major isoprenoid quinone present in strain NKC3-5T.
31659686	4	54	theme	optimum	448:454	arg1	pH					435:436	pH 6.5-10.0	435:445	pH 6.5-10.0 (optimum pH 9.0)	435:462	The strain NKC3-5T could grow at up to 20.0% (w/v) NaCl (optimum 10%), pH 6.5-10.0 (optimum pH 9.0), and 25-40°C (optimum 35°C).
31659686	4	54	theme	optimum	448:454	arg1	pH					456:457	optimum pH 9.0	448:461	optimum pH 9.0	448:461	The strain NKC3-5T could grow at up to 20.0% (w/v) NaCl (optimum 10%), pH 6.5-10.0 (optimum pH 9.0), and 25-40°C (optimum 35°C).
31659686	10	55	theme	carbohydrate	1503:1514	arg1	metabolism					1516:1525	carbohydrate metabolism	1503:1525	carbohydrate metabolism	1503:1525	Comparative genomic analysis revealed that strain NKC3-5T had 716 pan-genome orthologous groups (POGs), dominated with carbohydrate metabolism.
31659686	15	56	theme	chemotaxonomic	2083:2096	arg1	properties					2098:2107	The phephenotypic, genomic, phylogenetic, and chemotaxonomic properties	2037:2107	The phephenotypic, genomic, phylogenetic, and chemotaxonomic properties	2037:2107	The phephenotypic, genomic, phylogenetic, and chemotaxonomic properties reveal that the strain represents a novel species of the genus Salicibibacter, for which the name Salicibibacter halophilus sp.
31659686	13	57	theme	major	1864:1868	arg1	quinone					1881:1887	the major isoprenoid quinone	1860:1887	the major isoprenoid quinone present in strain NKC3-5T	1860:1913	Anteiso-C15:0, iso-C17:0, anteiso-C17:0, and iso-C15:0 were the major cellular fatty acids, and menaquinone-7 was the major isoprenoid quinone present in strain NKC3-5T.
31659686	13	57	theme	major	1864:1868	arg1	menaquinone-7					1842:1854	menaquinone-7	1842:1854	menaquinone-7	1842:1854	Anteiso-C15:0, iso-C17:0, anteiso-C17:0, and iso-C15:0 were the major cellular fatty acids, and menaquinone-7 was the major isoprenoid quinone present in strain NKC3-5T.
31659686	12	58	theme	predominant	1666:1676	arg1	phosphatidylglycerol					1696:1715	phosphatidylglycerol	1696:1715	phosphatidylglycerol	1696:1715	The predominant polar lipids were phosphatidylglycerol and two unidentified lipids.
31659686	12	58	theme	predominant	1666:1676	arg1	lipids					1684:1689	The predominant polar lipids	1662:1689	The predominant polar lipids	1662:1689	The predominant polar lipids were phosphatidylglycerol and two unidentified lipids.
31659686	7	59	theme	Bacillaceae	1053:1063	arg1	branch					1003:1008	a distinct branch	992:1008	a distinct branch with other published species of the family Bacillaceae	992:1063	Phylogenetic analysis based on 16S rRNA showed that strain NKC3-5T clustered with bacterium Salicibibacter kimchii NKC1-1T, with a similarity of 96.2-97.6%, but formed a distinct branch with other published species of the family Bacillaceae.
31659686	6	60	dep	size	623:626	arg1	The					612:614	The	612:614	The	612:614	The genome size and genomic G + C content of strain NKC3-5T were 3,754,174 bp and 45.9 mol%, respectively; it contained 3,630 coding sequences, 16S rRNA genes (six 16S, five 5S, and five 23S), and 59 tRNA genes.
31659686	6	61	theme	genome	616:621	arg1	size					623:626	genome size	616:626	genome size	616:626	The genome size and genomic G + C content of strain NKC3-5T were 3,754,174 bp and 45.9 mol%, respectively; it contained 3,630 coding sequences, 16S rRNA genes (six 16S, five 5S, and five 23S), and 59 tRNA genes.
31659686	4	62	dep	20.0	403:406	arg1	to					400:401	to	400:401	to	400:401	The strain NKC3-5T could grow at up to 20.0% (w/v) NaCl (optimum 10%), pH 6.5-10.0 (optimum pH 9.0), and 25-40°C (optimum 35°C).
31659686	16	63	theme	=	2285:2285	arg1	33437T					2305:2310	= KACC 21230T = JCM 33437T	2285:2310	= KACC 21230T = JCM 33437T	2285:2310	nov. is proposed, with the type strain NKC3-5T (= KACC 21230T = JCM 33437T).
31659686	16	63	theme	=	2285:2285	arg1	NKC3-5T					2276:2282	the type strain NKC3-5T	2260:2282	the type strain NKC3-5T (= KACC 21230T = JCM 33437T)	2260:2311	nov. is proposed, with the type strain NKC3-5T (= KACC 21230T = JCM 33437T).
31659686	5	64	theme	aerobic	537:543	arg1	conditions					545:554	aerobic conditions	537:554	aerobic conditions	537:554	The cells were able to reduce nitrate under aerobic conditions, which is the first report in the genus Salicibibacter.
31659686	16	65	theme	strain	2269:2274	arg1	33437T					2305:2310	= KACC 21230T = JCM 33437T	2285:2310	= KACC 21230T = JCM 33437T	2285:2310	nov. is proposed, with the type strain NKC3-5T (= KACC 21230T = JCM 33437T).
31659686	16	65	theme	strain	2269:2274	arg1	NKC3-5T					2276:2282	the type strain NKC3-5T	2260:2282	the type strain NKC3-5T (= KACC 21230T = JCM 33437T)	2260:2311	nov. is proposed, with the type strain NKC3-5T (= KACC 21230T = JCM 33437T).
31659686	6	66	theme	16S	756:758	arg1	16S					776:778	six 16S	772:778	six 16S	772:778	The genome size and genomic G + C content of strain NKC3-5T were 3,754,174 bp and 45.9 mol%, respectively; it contained 3,630 coding sequences, 16S rRNA genes (six 16S, five 5S, and five 23S), and 59 tRNA genes.
31659686	6	66	theme	16S	756:758	arg1	5S					786:787	five 5S	781:787	five 5S	781:787	The genome size and genomic G + C content of strain NKC3-5T were 3,754,174 bp and 45.9 mol%, respectively; it contained 3,630 coding sequences, 16S rRNA genes (six 16S, five 5S, and five 23S), and 59 tRNA genes.
31659686	6	66	theme	16S	756:758	arg1	23S					799:801	five 23S	794:801	five 23S	794:801	The genome size and genomic G + C content of strain NKC3-5T were 3,754,174 bp and 45.9 mol%, respectively; it contained 3,630 coding sequences, 16S rRNA genes (six 16S, five 5S, and five 23S), and 59 tRNA genes.
31659686	6	66	theme	16S	756:758	arg1	genes					765:769	16S rRNA genes	756:769	16S rRNA genes (six 16S, five 5S, and five 23S)	756:802	The genome size and genomic G + C content of strain NKC3-5T were 3,754,174 bp and 45.9 mol%, respectively; it contained 3,630 coding sequences, 16S rRNA genes (six 16S, five 5S, and five 23S), and 59 tRNA genes.
31659686	4	67	theme	optimum	478:484	arg1	25-40°C					469:475	25-40°C	469:475	25-40°C (optimum 35°C)	469:490	The strain NKC3-5T could grow at up to 20.0% (w/v) NaCl (optimum 10%), pH 6.5-10.0 (optimum pH 9.0), and 25-40°C (optimum 35°C).
31659686	4	67	theme	optimum	478:484	arg1	35°C					486:489	optimum 35°C	478:489	optimum 35°C	478:489	The strain NKC3-5T could grow at up to 20.0% (w/v) NaCl (optimum 10%), pH 6.5-10.0 (optimum pH 9.0), and 25-40°C (optimum 35°C).
31659686	9	68	theme	genome	1223:1228	arg1	annotation					1230:1239	functional genome annotation	1212:1239	functional genome annotation	1212:1239	Using functional genome annotation, the result found that carbohydrate, amino acid, and vitamin metabolism related genes were highly distributed in the genome of strain NKC3-5T.
31659686	16	69	theme	JCM	2301:2303	arg1	33437T					2305:2310	= KACC 21230T = JCM 33437T	2285:2310	= KACC 21230T = JCM 33437T	2285:2310	nov. is proposed, with the type strain NKC3-5T (= KACC 21230T = JCM 33437T).
31659686	16	69	theme	JCM	2301:2303	arg1	NKC3-5T					2276:2282	the type strain NKC3-5T	2260:2282	the type strain NKC3-5T (= KACC 21230T = JCM 33437T)	2260:2311	nov. is proposed, with the type strain NKC3-5T (= KACC 21230T = JCM 33437T).
31659686	13	70	theme	cellular	1816:1823	arg1	anteiso-C17:0					1772:1784	anteiso-C17:0	1772:1784	anteiso-C17:0	1772:1784	Anteiso-C15:0, iso-C17:0, anteiso-C17:0, and iso-C15:0 were the major cellular fatty acids, and menaquinone-7 was the major isoprenoid quinone present in strain NKC3-5T.
31659686	13	70	theme	cellular	1816:1823	arg1	iso-C15:0					1791:1799	iso-C15:0	1791:1799	iso-C15:0	1791:1799	Anteiso-C15:0, iso-C17:0, anteiso-C17:0, and iso-C15:0 were the major cellular fatty acids, and menaquinone-7 was the major isoprenoid quinone present in strain NKC3-5T.
31659686	13	70	theme	cellular	1816:1823	arg1	iso-C17:0					1761:1769	iso-C17:0	1761:1769	iso-C17:0	1761:1769	Anteiso-C15:0, iso-C17:0, anteiso-C17:0, and iso-C15:0 were the major cellular fatty acids, and menaquinone-7 was the major isoprenoid quinone present in strain NKC3-5T.
31659686	13	70	theme	cellular	1816:1823	arg1	acids					1831:1835	the major cellular fatty acids	1806:1835	the major cellular fatty acids	1806:1835	Anteiso-C15:0, iso-C17:0, anteiso-C17:0, and iso-C15:0 were the major cellular fatty acids, and menaquinone-7 was the major isoprenoid quinone present in strain NKC3-5T.
31659686	13	70	theme	cellular	1816:1823	arg1	Anteiso-C15:0					1746:1758	Anteiso-C15:0	1746:1758	Anteiso-C15:0	1746:1758	Anteiso-C15:0, iso-C17:0, anteiso-C17:0, and iso-C15:0 were the major cellular fatty acids, and menaquinone-7 was the major isoprenoid quinone present in strain NKC3-5T.
31659686	2	71	theme	Korea	282:286	arg1	Republic					270:277	the Republic	266:277	the Republic of Korea	266:286	A Gram-stain-positive, rod-shaped, alkalitolerant, and halophilic bacterium-designated as strain NKC3-5T-was isolated from kimchi that was collected from the Geumsan area in the Republic of Korea.
31659686	6	72	theme	3,630	732:736	arg1	sequences					745:753	3,630 coding sequences	732:753	3,630 coding sequences	732:753	The genome size and genomic G + C content of strain NKC3-5T were 3,754,174 bp and 45.9 mol%, respectively; it contained 3,630 coding sequences, 16S rRNA genes (six 16S, five 5S, and five 23S), and 59 tRNA genes.
31659686	13	73	from	present	1889:1895	arg1	NKC3-5T					1907:1913	strain NKC3-5T	1900:1913	strain NKC3-5T	1900:1913	Anteiso-C15:0, iso-C17:0, anteiso-C17:0, and iso-C15:0 were the major cellular fatty acids, and menaquinone-7 was the major isoprenoid quinone present in strain NKC3-5T.
31659686	10	74	theme	Comparative	1384:1394	arg1	analysis					1404:1411	Comparative genomic analysis	1384:1411	Comparative genomic analysis	1384:1411	Comparative genomic analysis revealed that strain NKC3-5T had 716 pan-genome orthologous groups (POGs), dominated with carbohydrate metabolism.
31659686	6	75	theme	C	644:644	arg1	content					646:652	genomic G + C content	632:652	content	646:652	The genome size and genomic G + C content of strain NKC3-5T were 3,754,174 bp and 45.9 mol%, respectively; it contained 3,630 coding sequences, 16S rRNA genes (six 16S, five 5S, and five 23S), and 59 tRNA genes.
31659686	5	76	theme	genus	590:594	arg1	Salicibibacter					596:609	the genus Salicibibacter	586:609	the genus Salicibibacter	586:609	The cells were able to reduce nitrate under aerobic conditions, which is the first report in the genus Salicibibacter.
31659686	2	77	theme	Geumsan	250:256	arg1	area					258:261	the Geumsan area	246:261	the Geumsan area in the Republic of Korea	246:286	A Gram-stain-positive, rod-shaped, alkalitolerant, and halophilic bacterium-designated as strain NKC3-5T-was isolated from kimchi that was collected from the Geumsan area in the Republic of Korea.
31659686	14	78	theme	diamino	2023:2029	arg1	acid					2031:2034	the diagnostic diamino acid	2008:2034	the diagnostic diamino acid	2008:2034	Cell wall peptidoglycan analysis of strain NKC3-5T showed that meso-diaminopimelic acid was the diagnostic diamino acid.
31659686	14	78	theme	diamino	2023:2029	arg1	acid					1999:2002	meso-diaminopimelic acid	1979:2002	meso-diaminopimelic acid	1979:2002	Cell wall peptidoglycan analysis of strain NKC3-5T showed that meso-diaminopimelic acid was the diagnostic diamino acid.
31659686	9	79	theme	acid	1284:1287	arg1	genes					1321:1325	carbohydrate, amino acid, and vitamin metabolism related genes	1264:1325	carbohydrate, amino acid, and vitamin metabolism related genes	1264:1325	Using functional genome annotation, the result found that carbohydrate, amino acid, and vitamin metabolism related genes were highly distributed in the genome of strain NKC3-5T.
31659686	3	80	theme	NKC3-5T	314:320	arg1	Cells					289:293	Cells	289:293	Cells of isolated strain NKC3-5T	289:320	Cells of isolated strain NKC3-5T were 0.5-0.7 μm wide and 1.4-2.8 μm long.
31659686	5	81	theme	first	570:574	arg1	report					576:581	the first report	566:581	the first report in the genus Salicibibacter	566:609	The cells were able to reduce nitrate under aerobic conditions, which is the first report in the genus Salicibibacter.
31659686	5	81	theme	first	570:574	arg1	which					557:561	which	557:561	which	557:561	The cells were able to reduce nitrate under aerobic conditions, which is the first report in the genus Salicibibacter.
31659686	15	82	theme	Salicibibacter	2207:2220	arg1	sp					2233:2234	the name Salicibibacter halophilus sp	2198:2234	the name Salicibibacter halophilus sp	2198:2234	The phephenotypic, genomic, phylogenetic, and chemotaxonomic properties reveal that the strain represents a novel species of the genus Salicibibacter, for which the name Salicibibacter halophilus sp.
31659686	3	83	theme	isolated	298:305	arg1	NKC3-5T					314:320	isolated strain NKC3-5T	298:320	isolated strain NKC3-5T	298:320	Cells of isolated strain NKC3-5T were 0.5-0.7 μm wide and 1.4-2.8 μm long.
31659686	7	84	with	branch	1003:1008	arg1	species					1031:1037	other published species	1015:1037	other published species	1015:1037	Phylogenetic analysis based on 16S rRNA showed that strain NKC3-5T clustered with bacterium Salicibibacter kimchii NKC1-1T, with a similarity of 96.2-97.6%, but formed a distinct branch with other published species of the family Bacillaceae.
31659686	8	85	theme	OrthoANI	1079:1086	arg1	value					1088:1092	OrthoANI value	1079:1092	OrthoANI value between strain NKC3-5T and Salicibibacter kimchii NKC1-1T	1079:1150	In addition, OrthoANI value between strain NKC3-5T and Salicibibacter kimchii NKC1-1T was far lower than the species demarcation threshold.
31659686	11	86	theme	concatenated	1563:1574	arg1	POGs					1581:1584	the concatenated core POGs	1559:1584	the concatenated core POGs	1559:1584	Phylogenomic analysis based on the concatenated core POGs revealed that strain NKC3-5T was closely related to Salicibibacter kimchii.
31659686	9	87	theme	metabolism	1302:1311	arg1	genes					1321:1325	carbohydrate, amino acid, and vitamin metabolism related genes	1264:1325	carbohydrate, amino acid, and vitamin metabolism related genes	1264:1325	Using functional genome annotation, the result found that carbohydrate, amino acid, and vitamin metabolism related genes were highly distributed in the genome of strain NKC3-5T.
31659686	15	88	theme	genus	2166:2170	arg1	Salicibibacter					2172:2185	the genus Salicibibacter	2162:2185	the genus Salicibibacter	2162:2185	The phephenotypic, genomic, phylogenetic, and chemotaxonomic properties reveal that the strain represents a novel species of the genus Salicibibacter, for which the name Salicibibacter halophilus sp.
31659686	7	89	theme	distinct	994:1001	arg1	branch					1003:1008	a distinct branch	992:1008	a distinct branch with other published species of the family Bacillaceae	992:1063	Phylogenetic analysis based on 16S rRNA showed that strain NKC3-5T clustered with bacterium Salicibibacter kimchii NKC1-1T, with a similarity of 96.2-97.6%, but formed a distinct branch with other published species of the family Bacillaceae.
31659686	1	90	theme	halophilic	49:58	arg1	bacterium					60:68	a moderately halophilic bacterium	36:68	a moderately halophilic bacterium	36:68	nov., a moderately halophilic bacterium isolated from kimchi.
31659686	1	90	theme	halophilic	49:58	arg1	nov.					30:33	nov.	30:33	nov.	30:33	nov., a moderately halophilic bacterium isolated from kimchi.
31659686	16	91	theme	type	2264:2267	arg1	33437T					2305:2310	= KACC 21230T = JCM 33437T	2285:2310	= KACC 21230T = JCM 33437T	2285:2310	nov. is proposed, with the type strain NKC3-5T (= KACC 21230T = JCM 33437T).
31659686	16	91	theme	type	2264:2267	arg1	NKC3-5T					2276:2282	the type strain NKC3-5T	2260:2282	the type strain NKC3-5T (= KACC 21230T = JCM 33437T)	2260:2311	nov. is proposed, with the type strain NKC3-5T (= KACC 21230T = JCM 33437T).
31659686	14	92	theme	strain	1952:1957	arg1	NKC3-5T					1959:1965	strain NKC3-5T	1952:1965	strain NKC3-5T	1952:1965	Cell wall peptidoglycan analysis of strain NKC3-5T showed that meso-diaminopimelic acid was the diagnostic diamino acid.
31659686	8	93	theme	Salicibibacter	1121:1134	arg1	NKC1-1T					1144:1150	Salicibibacter kimchii NKC1-1T	1121:1150	Salicibibacter kimchii NKC1-1T	1121:1150	In addition, OrthoANI value between strain NKC3-5T and Salicibibacter kimchii NKC1-1T was far lower than the species demarcation threshold.
31659686	12	94	theme	unidentified	1725:1736	arg1	lipids					1738:1743	two unidentified lipids	1721:1743	two unidentified lipids	1721:1743	The predominant polar lipids were phosphatidylglycerol and two unidentified lipids.
31659686	5	95	from	report	576:581	arg1	Salicibibacter					596:609	the genus Salicibibacter	586:609	the genus Salicibibacter	586:609	The cells were able to reduce nitrate under aerobic conditions, which is the first report in the genus Salicibibacter.
31659686	10	96	theme	pan-genome	1450:1459	arg1	POGs					1481:1484	POGs	1481:1484	POGs	1481:1484	Comparative genomic analysis revealed that strain NKC3-5T had 716 pan-genome orthologous groups (POGs), dominated with carbohydrate metabolism.
31659686	10	96	theme	pan-genome	1450:1459	arg1	groups					1473:1478	716 pan-genome orthologous groups	1446:1478	716 pan-genome orthologous groups (POGs)	1446:1485	Comparative genomic analysis revealed that strain NKC3-5T had 716 pan-genome orthologous groups (POGs), dominated with carbohydrate metabolism.
31659686	0	97	theme	halophilus	15:24	arg1	sp					26:27	Salicibibacter halophilus sp	0:27	Salicibibacter halophilus sp.	0:28	Salicibibacter halophilus sp.
31659686	14	98	theme	wall	1921:1924	arg1	analysis					1940:1947	Cell wall peptidoglycan analysis	1916:1947	Cell wall peptidoglycan analysis of strain NKC3-5T	1916:1965	Cell wall peptidoglycan analysis of strain NKC3-5T showed that meso-diaminopimelic acid was the diagnostic diamino acid.
31659686	7	99	theme	Salicibibacter	916:929	arg1	NKC1-1T					939:945	bacterium Salicibibacter kimchii NKC1-1T	906:945	bacterium Salicibibacter kimchii NKC1-1T	906:945	Phylogenetic analysis based on 16S rRNA showed that strain NKC3-5T clustered with bacterium Salicibibacter kimchii NKC1-1T, with a similarity of 96.2-97.6%, but formed a distinct branch with other published species of the family Bacillaceae.
31659686	9	100	theme	strain	1368:1373	arg1	NKC3-5T					1375:1381	strain NKC3-5T	1368:1381	strain NKC3-5T	1368:1381	Using functional genome annotation, the result found that carbohydrate, amino acid, and vitamin metabolism related genes were highly distributed in the genome of strain NKC3-5T.
31659686	2	101	from	area	258:261	arg1	Republic					270:277	the Republic	266:277	the Republic of Korea	266:286	A Gram-stain-positive, rod-shaped, alkalitolerant, and halophilic bacterium-designated as strain NKC3-5T-was isolated from kimchi that was collected from the Geumsan area in the Republic of Korea.
31659686	2	102	theme	strain	182:187	arg1	NKC3-5T-was					189:199	strain NKC3-5T-was	182:199	strain NKC3-5T-was	182:199	A Gram-stain-positive, rod-shaped, alkalitolerant, and halophilic bacterium-designated as strain NKC3-5T-was isolated from kimchi that was collected from the Geumsan area in the Republic of Korea.
31659686	8	103	theme	species	1175:1181	arg1	threshold					1195:1203	the species demarcation threshold	1171:1203	the species demarcation threshold	1171:1203	In addition, OrthoANI value between strain NKC3-5T and Salicibibacter kimchii NKC1-1T was far lower than the species demarcation threshold.
31659686	11	104	theme	Phylogenomic	1528:1539	arg1	analysis					1541:1548	Phylogenomic analysis	1528:1548	Phylogenomic analysis based on the concatenated core POGs	1528:1584	Phylogenomic analysis based on the concatenated core POGs revealed that strain NKC3-5T was closely related to Salicibibacter kimchii.
31659686	6	105	theme	genomic	632:638	arg1	G					640:640	genomic G + C content	632:652	G	640:640	The genome size and genomic G + C content of strain NKC3-5T were 3,754,174 bp and 45.9 mol%, respectively; it contained 3,630 coding sequences, 16S rRNA genes (six 16S, five 5S, and five 23S), and 59 tRNA genes.
31659686	12	106	theme	polar	1678:1682	arg1	phosphatidylglycerol					1696:1715	phosphatidylglycerol	1696:1715	phosphatidylglycerol	1696:1715	The predominant polar lipids were phosphatidylglycerol and two unidentified lipids.
31659686	12	106	theme	polar	1678:1682	arg1	lipids					1684:1689	The predominant polar lipids	1662:1689	The predominant polar lipids	1662:1689	The predominant polar lipids were phosphatidylglycerol and two unidentified lipids.
31659686	6	107	theme	mol	699:701	arg1	%					702:702	3,754,174 bp and 45.9 mol%	677:702	%	702:702	The genome size and genomic G + C content of strain NKC3-5T were 3,754,174 bp and 45.9 mol%, respectively; it contained 3,630 coding sequences, 16S rRNA genes (six 16S, five 5S, and five 23S), and 59 tRNA genes.
31659686	15	108	theme	novel	2145:2149	arg1	species					2151:2157	a novel species	2143:2157	a novel species	2143:2157	The phephenotypic, genomic, phylogenetic, and chemotaxonomic properties reveal that the strain represents a novel species of the genus Salicibibacter, for which the name Salicibibacter halophilus sp.
31659686	7	109	theme	family	1046:1051	arg1	Bacillaceae					1053:1063	the family Bacillaceae	1042:1063	the family Bacillaceae	1042:1063	Phylogenetic analysis based on 16S rRNA showed that strain NKC3-5T clustered with bacterium Salicibibacter kimchii NKC1-1T, with a similarity of 96.2-97.6%, but formed a distinct branch with other published species of the family Bacillaceae.
31659686	13	110	theme	fatty	1825:1829	arg1	anteiso-C17:0					1772:1784	anteiso-C17:0	1772:1784	anteiso-C17:0	1772:1784	Anteiso-C15:0, iso-C17:0, anteiso-C17:0, and iso-C15:0 were the major cellular fatty acids, and menaquinone-7 was the major isoprenoid quinone present in strain NKC3-5T.
31659686	13	110	theme	fatty	1825:1829	arg1	iso-C15:0					1791:1799	iso-C15:0	1791:1799	iso-C15:0	1791:1799	Anteiso-C15:0, iso-C17:0, anteiso-C17:0, and iso-C15:0 were the major cellular fatty acids, and menaquinone-7 was the major isoprenoid quinone present in strain NKC3-5T.
31659686	13	110	theme	fatty	1825:1829	arg1	iso-C17:0					1761:1769	iso-C17:0	1761:1769	iso-C17:0	1761:1769	Anteiso-C15:0, iso-C17:0, anteiso-C17:0, and iso-C15:0 were the major cellular fatty acids, and menaquinone-7 was the major isoprenoid quinone present in strain NKC3-5T.
31659686	13	110	theme	fatty	1825:1829	arg1	acids					1831:1835	the major cellular fatty acids	1806:1835	the major cellular fatty acids	1806:1835	Anteiso-C15:0, iso-C17:0, anteiso-C17:0, and iso-C15:0 were the major cellular fatty acids, and menaquinone-7 was the major isoprenoid quinone present in strain NKC3-5T.
31659686	13	110	theme	fatty	1825:1829	arg1	Anteiso-C15:0					1746:1758	Anteiso-C15:0	1746:1758	Anteiso-C15:0	1746:1758	Anteiso-C15:0, iso-C17:0, anteiso-C17:0, and iso-C15:0 were the major cellular fatty acids, and menaquinone-7 was the major isoprenoid quinone present in strain NKC3-5T.
31659686	4	111	dep	%	407:407	arg1	20.0					403:406	20.0	403:406	20.0	403:406	The strain NKC3-5T could grow at up to 20.0% (w/v) NaCl (optimum 10%), pH 6.5-10.0 (optimum pH 9.0), and 25-40°C (optimum 35°C).
31659686	4	111	dep	%	407:407	arg1	w/v					410:412	w/v	410:412	w/v	410:412	The strain NKC3-5T could grow at up to 20.0% (w/v) NaCl (optimum 10%), pH 6.5-10.0 (optimum pH 9.0), and 25-40°C (optimum 35°C).
31659686	15	112	theme	Salicibibacter	2172:2185	arg1	species					2151:2157	a novel species	2143:2157	a novel species	2143:2157	The phephenotypic, genomic, phylogenetic, and chemotaxonomic properties reveal that the strain represents a novel species of the genus Salicibibacter, for which the name Salicibibacter halophilus sp.
30988615	9	0	theme	human	1650:1654	arg1	HDF					1675:1677	human dermal fibroblast (HDF) and human melanoma cells	1650:1703	HDF	1675:1677	In addition, the NPs were added to the growth media of human dermal fibroblast (HDF) and human melanoma cells to show their cytocompatibility and cytotoxicity, respectively, over a 3-day experiment.
30988615	6	1	theme	Transmission	1080:1091	arg1	TEM					1114:1116	TEM	1114:1116	TEM	1114:1116	Transmission electron microscopy (TEM) imaging was done to determine the morphology and size of the nanostructures.
30988615	6	1	theme	Transmission	1080:1091	arg1	microscopy					1102:1111	Transmission electron microscopy	1080:1111	Transmission electron microscopy (TEM) imaging	1080:1125	Transmission electron microscopy (TEM) imaging was done to determine the morphology and size of the nanostructures.
30988615	2	2	theme	nanoparticles	612:624	arg1	synthesis					556:564	a highly reproducible one-pot green synthesis	520:564	a highly reproducible one-pot green synthesis of colloidal AuNPs and bimetallic Ag/Au alloy nanoparticles (NPs; Ag/AuNPs) using starch as reducing and capping agent	520:683	Thus, the aim of this research was to establish a highly reproducible one-pot green synthesis of colloidal AuNPs and bimetallic Ag/Au alloy nanoparticles (NPs; Ag/AuNPs) using starch as reducing and capping agent.
30988615	11	3	theme	nm	2030:2031	arg1	sizes					2008:2012	mean particle sizes	1994:2012	mean particle sizes of 28.5 and 9.7 nm for AuNPs and Ag/AuNPs, respectively	1994:2068	The NPs showed a face-centered cubic crystal structure and an icosahedral shape with mean particle sizes of 28.5 and 9.7 nm for AuNPs and Ag/AuNPs, respectively.
30988615	1	4	theme	nanoparticles	325:337	arg1	biocompatibility					300:315	the general biocompatibility	288:315	the general biocompatibility of gold nanoparticles (AuNPs)	288:345	BACKGROUND AND AIM Bimetallic silver/gold nanosystems are expected to significantly improve therapeutic efficacy compared to their monometallic counterparts by maintaining the general biocompatibility of gold nanoparticles (AuNPs) while, at the same time, decreasing the relatively high toxicity of silver nanoparticles (AgNPs) toward healthy human cells.
30988615	9	5	theme	HDF	1675:1677	arg1	media					1641:1645	the growth media	1630:1645	the growth media of human dermal fibroblast (HDF) and human melanoma cells	1630:1703	In addition, the NPs were added to the growth media of human dermal fibroblast (HDF) and human melanoma cells to show their cytocompatibility and cytotoxicity, respectively, over a 3-day experiment.
30988615	3	6	theme	high	718:721	arg1	reproducibility					723:737	high reproducibility	718:737	high reproducibility	718:737	METHODS The optical properties, high reproducibility, stability and particle size distribution of the colloidal NPs were analyzed by ultraviolet (UV)-visible spectroscopy, dynamic light scattering (DLS) and ζ-potential.
30988615	1	7	theme	therapeutic	208:218	arg1	efficacy					220:227	therapeutic efficacy	208:227	therapeutic efficacy compared to their monometallic counterparts	208:271	BACKGROUND AND AIM Bimetallic silver/gold nanosystems are expected to significantly improve therapeutic efficacy compared to their monometallic counterparts by maintaining the general biocompatibility of gold nanoparticles (AuNPs) while, at the same time, decreasing the relatively high toxicity of silver nanoparticles (AgNPs) toward healthy human cells.
30988615	14	8	theme	first	2485:2489	arg1	time					2491:2494	the first time	2481:2494	the first time using a highly reproducible, environmentally friendly, cost-effective and quick method	2481:2581	CONCLUSION In this study, mono- and bimetallic NPs were synthesized for the first time using a highly reproducible, environmentally friendly, cost-effective and quick method and were successfully characterized and tested for several anti-infection and anticancer biomedical applications.
30988615	2	9	theme	bimetallic	589:598	arg1	alloy					606:610	bimetallic Ag/Au alloy	589:610	bimetallic Ag/Au alloy	589:610	Thus, the aim of this research was to establish a highly reproducible one-pot green synthesis of colloidal AuNPs and bimetallic Ag/Au alloy nanoparticles (NPs; Ag/AuNPs) using starch as reducing and capping agent.
30988615	12	10	theme	dose-dependent	2167:2180	arg1	behavior					2196:2203	a dose-dependent antimicrobial behavior	2165:2203	a dose-dependent antimicrobial behavior	2165:2203	The antibacterial studies of the NPs against antibiotic-resistant bacterial strains presented a dose-dependent antimicrobial behavior.
30988615	3	11	theme	size	763:766	arg1	distribution					768:779	particle size distribution	754:779	particle size distribution	754:779	METHODS The optical properties, high reproducibility, stability and particle size distribution of the colloidal NPs were analyzed by ultraviolet (UV)-visible spectroscopy, dynamic light scattering (DLS) and ζ-potential.
30988615	9	12	theme	melanoma	1690:1697	arg1	cells					1699:1703	human dermal fibroblast (HDF) and human melanoma cells	1650:1703	cells	1699:1703	In addition, the NPs were added to the growth media of human dermal fibroblast (HDF) and human melanoma cells to show their cytocompatibility and cytotoxicity, respectively, over a 3-day experiment.
30988615	14	13	from	CONCLUSION	2409:2418	arg1	study					2428:2432	this study	2423:2432	this study	2423:2432	CONCLUSION In this study, mono- and bimetallic NPs were synthesized for the first time using a highly reproducible, environmentally friendly, cost-effective and quick method and were successfully characterized and tested for several anti-infection and anticancer biomedical applications.
30988615	1	14	theme	silver	415:420	arg1	AgNPs					437:441	AgNPs	437:441	AgNPs	437:441	BACKGROUND AND AIM Bimetallic silver/gold nanosystems are expected to significantly improve therapeutic efficacy compared to their monometallic counterparts by maintaining the general biocompatibility of gold nanoparticles (AuNPs) while, at the same time, decreasing the relatively high toxicity of silver nanoparticles (AgNPs) toward healthy human cells.
30988615	1	14	theme	silver	415:420	arg1	nanoparticles					422:434	silver nanoparticles	415:434	silver nanoparticles (AgNPs)	415:442	BACKGROUND AND AIM Bimetallic silver/gold nanosystems are expected to significantly improve therapeutic efficacy compared to their monometallic counterparts by maintaining the general biocompatibility of gold nanoparticles (AuNPs) while, at the same time, decreasing the relatively high toxicity of silver nanoparticles (AgNPs) toward healthy human cells.
30988615	1	15	theme	Bimetallic	135:144	arg1	nanosystems					158:168	Bimetallic silver/gold nanosystems	135:168	Bimetallic silver/gold nanosystems	135:168	BACKGROUND AND AIM Bimetallic silver/gold nanosystems are expected to significantly improve therapeutic efficacy compared to their monometallic counterparts by maintaining the general biocompatibility of gold nanoparticles (AuNPs) while, at the same time, decreasing the relatively high toxicity of silver nanoparticles (AgNPs) toward healthy human cells.
30988615	4	16	theme	capping	932:938	arg1	agent					940:944	capping agent	932:944	capping agent	932:944	The presence of starch as capping agent was determined by Fourier transform infrared (FT-IR) spectroscopy.
30988615	12	17	theme	bacterial	2137:2145	arg1	strains					2147:2153	antibiotic-resistant bacterial strains	2116:2153	antibiotic-resistant bacterial strains	2116:2153	The antibacterial studies of the NPs against antibiotic-resistant bacterial strains presented a dose-dependent antimicrobial behavior.
30988615	3	18	theme	colloidal	788:796	arg1	NPs					798:800	the colloidal NPs	784:800	the colloidal NPs	784:800	METHODS The optical properties, high reproducibility, stability and particle size distribution of the colloidal NPs were analyzed by ultraviolet (UV)-visible spectroscopy, dynamic light scattering (DLS) and ζ-potential.
30988615	2	19	dep	NPs	627:629	arg1	Ag/AuNPs					632:639	Ag/AuNPs	632:639	NPs; Ag/AuNPs	627:639	Thus, the aim of this research was to establish a highly reproducible one-pot green synthesis of colloidal AuNPs and bimetallic Ag/Au alloy nanoparticles (NPs; Ag/AuNPs) using starch as reducing and capping agent.
30988615	2	20	theme	Ag/Au	600:604	arg1	alloy					606:610	bimetallic Ag/Au alloy	589:610	bimetallic Ag/Au alloy	589:610	Thus, the aim of this research was to establish a highly reproducible one-pot green synthesis of colloidal AuNPs and bimetallic Ag/Au alloy nanoparticles (NPs; Ag/AuNPs) using starch as reducing and capping agent.
30988615	4	21	dep	transform	972:980	arg1	infrared					982:989	infrared	982:989	transform infrared (FT-IR) spectroscopy	972:1010	The presence of starch as capping agent was determined by Fourier transform infrared (FT-IR) spectroscopy.
30988615	11	22	theme	icosahedral	1971:1981	arg1	shape					1983:1987	an icosahedral shape	1968:1987	an icosahedral shape	1968:1987	The NPs showed a face-centered cubic crystal structure and an icosahedral shape with mean particle sizes of 28.5 and 9.7 nm for AuNPs and Ag/AuNPs, respectively.
30988615	9	23	theme	fibroblast	1663:1672	arg1	HDF					1675:1677	human dermal fibroblast (HDF) and human melanoma cells	1650:1703	HDF	1675:1677	In addition, the NPs were added to the growth media of human dermal fibroblast (HDF) and human melanoma cells to show their cytocompatibility and cytotoxicity, respectively, over a 3-day experiment.
30988615	3	24	theme	dynamic	858:864	arg1	DLS					884:886	DLS	884:886	DLS	884:886	METHODS The optical properties, high reproducibility, stability and particle size distribution of the colloidal NPs were analyzed by ultraviolet (UV)-visible spectroscopy, dynamic light scattering (DLS) and ζ-potential.
30988615	3	24	theme	dynamic	858:864	arg1	scattering					872:881	dynamic light scattering	858:881	dynamic light scattering (DLS)	858:887	METHODS The optical properties, high reproducibility, stability and particle size distribution of the colloidal NPs were analyzed by ultraviolet (UV)-visible spectroscopy, dynamic light scattering (DLS) and ζ-potential.
30988615	14	25	theme	anticancer	2661:2670	arg1	applications					2683:2694	several anti-infection and anticancer biomedical applications	2634:2694	several anti-infection and anticancer biomedical applications	2634:2694	CONCLUSION In this study, mono- and bimetallic NPs were synthesized for the first time using a highly reproducible, environmentally friendly, cost-effective and quick method and were successfully characterized and tested for several anti-infection and anticancer biomedical applications.
30988615	1	26	theme	healthy	451:457	arg1	cells					465:469	healthy human cells	451:469	healthy human cells	451:469	BACKGROUND AND AIM Bimetallic silver/gold nanosystems are expected to significantly improve therapeutic efficacy compared to their monometallic counterparts by maintaining the general biocompatibility of gold nanoparticles (AuNPs) while, at the same time, decreasing the relatively high toxicity of silver nanoparticles (AgNPs) toward healthy human cells.
30988615	10	27	theme	reproducible	1847:1858	arg1	synthesis					1866:1874	the highly reproducible green synthesis	1836:1874	the highly reproducible green synthesis of colloidal AuNPs and Ag/AuNPs	1836:1906	RESULTS UV-visible spectroscopy confirmed the highly reproducible green synthesis of colloidal AuNPs and Ag/AuNPs.
30988615	14	28	theme	anti-infection	2642:2655	arg1	applications					2683:2694	several anti-infection and anticancer biomedical applications	2634:2694	several anti-infection and anticancer biomedical applications	2634:2694	CONCLUSION In this study, mono- and bimetallic NPs were synthesized for the first time using a highly reproducible, environmentally friendly, cost-effective and quick method and were successfully characterized and tested for several anti-infection and anticancer biomedical applications.
30988615	7	29	theme	spectrometry	1339:1350	arg1	analysis					1361:1368	inductively coupled plasma mass spectrometry (ICP-MS) analysis	1307:1368	inductively coupled plasma mass spectrometry (ICP-MS) analysis	1307:1368	The chemical composition of the nanomaterials was determined by energy-dispersive X-ray spectroscopy (EDS) and inductively coupled plasma mass spectrometry (ICP-MS) analysis.
30988615	1	30	dep	BACKGROUND	116:125	arg1	nanosystems					158:168	Bimetallic silver/gold nanosystems	135:168	Bimetallic silver/gold nanosystems	135:168	BACKGROUND AND AIM Bimetallic silver/gold nanosystems are expected to significantly improve therapeutic efficacy compared to their monometallic counterparts by maintaining the general biocompatibility of gold nanoparticles (AuNPs) while, at the same time, decreasing the relatively high toxicity of silver nanoparticles (AgNPs) toward healthy human cells.
30988615	11	31	theme	particle	1999:2006	arg1	sizes					2008:2012	mean particle sizes	1994:2012	mean particle sizes of 28.5 and 9.7 nm for AuNPs and Ag/AuNPs, respectively	1994:2068	The NPs showed a face-centered cubic crystal structure and an icosahedral shape with mean particle sizes of 28.5 and 9.7 nm for AuNPs and Ag/AuNPs, respectively.
30988615	7	32	theme	chemical	1200:1207	arg1	composition					1209:1219	The chemical composition	1196:1219	The chemical composition of the nanomaterials	1196:1240	The chemical composition of the nanomaterials was determined by energy-dispersive X-ray spectroscopy (EDS) and inductively coupled plasma mass spectrometry (ICP-MS) analysis.
30988615	2	33	theme	one-pot	542:548	arg1	synthesis					556:564	a highly reproducible one-pot green synthesis	520:564	a highly reproducible one-pot green synthesis of colloidal AuNPs and bimetallic Ag/Au alloy nanoparticles (NPs; Ag/AuNPs) using starch as reducing and capping agent	520:683	Thus, the aim of this research was to establish a highly reproducible one-pot green synthesis of colloidal AuNPs and bimetallic Ag/Au alloy nanoparticles (NPs; Ag/AuNPs) using starch as reducing and capping agent.
30988615	14	34	from	mono-	2435:2439	arg1	study					2428:2432	this study	2423:2432	this study	2423:2432	CONCLUSION In this study, mono- and bimetallic NPs were synthesized for the first time using a highly reproducible, environmentally friendly, cost-effective and quick method and were successfully characterized and tested for several anti-infection and anticancer biomedical applications.
30988615	2	35	theme	reducing	658:665	arg1	agent					679:683	reducing and capping agent	658:683	agent	679:683	Thus, the aim of this research was to establish a highly reproducible one-pot green synthesis of colloidal AuNPs and bimetallic Ag/Au alloy nanoparticles (NPs; Ag/AuNPs) using starch as reducing and capping agent.
30988615	13	36	theme	melanoma	2326:2333	arg1	cells					2335:2339	human melanoma cells	2320:2339	human melanoma cells	2320:2339	Furthermore, the NPs showed cytocompat-ibility towards HDF, but a dose-dependent anticancer effect was found when human melanoma cells were grown in presence of different NP concentrations for 72 hours.
30988615	3	37	dep	METHODS	686:692	arg1	analyzed					807:814	analyzed	807:814	were analyzed by ultraviolet (UV)-visible spectroscopy, dynamic light scattering (DLS) and ζ-potential	802:903	METHODS The optical properties, high reproducibility, stability and particle size distribution of the colloidal NPs were analyzed by ultraviolet (UV)-visible spectroscopy, dynamic light scattering (DLS) and ζ-potential.
30988615	7	38	theme	mass	1334:1337	arg1	ICP-MS					1353:1358	ICP-MS	1353:1358	ICP-MS	1353:1358	The chemical composition of the nanomaterials was determined by energy-dispersive X-ray spectroscopy (EDS) and inductively coupled plasma mass spectrometry (ICP-MS) analysis.
30988615	7	38	theme	mass	1334:1337	arg1	spectrometry					1339:1350	plasma mass spectrometry	1327:1350	inductively coupled plasma mass spectrometry (ICP-MS) analysis	1307:1368	The chemical composition of the nanomaterials was determined by energy-dispersive X-ray spectroscopy (EDS) and inductively coupled plasma mass spectrometry (ICP-MS) analysis.
30988615	13	39	theme	anticancer	2287:2296	arg1	effect					2298:2303	a dose-dependent anticancer effect	2270:2303	a dose-dependent anticancer effect	2270:2303	Furthermore, the NPs showed cytocompat-ibility towards HDF, but a dose-dependent anticancer effect was found when human melanoma cells were grown in presence of different NP concentrations for 72 hours.
30988615	0	40	theme	Starch-mediated	0:14	arg1	synthesis					16:24	Starch-mediated synthesis	0:24	Starch-mediated synthesis of mono- and bimetallic silver/gold	0:60	Starch-mediated synthesis of mono- and bimetallic silver/gold nanoparticles as antimicrobial and anticancer agents.
30988615	1	41	theme	human	459:463	arg1	cells					465:469	healthy human cells	451:469	healthy human cells	451:469	BACKGROUND AND AIM Bimetallic silver/gold nanosystems are expected to significantly improve therapeutic efficacy compared to their monometallic counterparts by maintaining the general biocompatibility of gold nanoparticles (AuNPs) while, at the same time, decreasing the relatively high toxicity of silver nanoparticles (AgNPs) toward healthy human cells.
30988615	7	42	theme	coupled	1319:1325	arg1	analysis					1361:1368	inductively coupled plasma mass spectrometry (ICP-MS) analysis	1307:1368	inductively coupled plasma mass spectrometry (ICP-MS) analysis	1307:1368	The chemical composition of the nanomaterials was determined by energy-dispersive X-ray spectroscopy (EDS) and inductively coupled plasma mass spectrometry (ICP-MS) analysis.
30988615	14	43	from	NPs	2456:2458	arg1	study					2428:2432	this study	2423:2432	this study	2423:2432	CONCLUSION In this study, mono- and bimetallic NPs were synthesized for the first time using a highly reproducible, environmentally friendly, cost-effective and quick method and were successfully characterized and tested for several anti-infection and anticancer biomedical applications.
30988615	2	44	theme	capping	671:677	arg1	agent					679:683	reducing and capping agent	658:683	agent	679:683	Thus, the aim of this research was to establish a highly reproducible one-pot green synthesis of colloidal AuNPs and bimetallic Ag/Au alloy nanoparticles (NPs; Ag/AuNPs) using starch as reducing and capping agent.
30988615	13	45	theme	dose-dependent	2272:2285	arg1	effect					2298:2303	a dose-dependent anticancer effect	2270:2303	a dose-dependent anticancer effect	2270:2303	Furthermore, the NPs showed cytocompat-ibility towards HDF, but a dose-dependent anticancer effect was found when human melanoma cells were grown in presence of different NP concentrations for 72 hours.
30988615	4	46	theme	FT-IR	992:996	arg1	spectroscopy					999:1010	(FT-IR) spectroscopy	991:1010	(FT-IR) spectroscopy	991:1010	The presence of starch as capping agent was determined by Fourier transform infrared (FT-IR) spectroscopy.
30988615	10	47	theme	colloidal	1879:1887	arg1	AuNPs					1889:1893	colloidal AuNPs	1879:1893	colloidal AuNPs	1879:1893	RESULTS UV-visible spectroscopy confirmed the highly reproducible green synthesis of colloidal AuNPs and Ag/AuNPs.
30988615	13	48	dep	concentrations	2380:2393	arg1	presence					2355:2362	presence	2355:2362	presence	2355:2362	Furthermore, the NPs showed cytocompat-ibility towards HDF, but a dose-dependent anticancer effect was found when human melanoma cells were grown in presence of different NP concentrations for 72 hours.
30988615	11	49	theme	cubic	1940:1944	arg1	structure					1954:1962	a face-centered cubic crystal structure	1924:1962	a face-centered cubic crystal structure	1924:1962	The NPs showed a face-centered cubic crystal structure and an icosahedral shape with mean particle sizes of 28.5 and 9.7 nm for AuNPs and Ag/AuNPs, respectively.
30988615	0	50	theme	silver/gold	50:60	arg1	synthesis					16:24	Starch-mediated synthesis	0:24	Starch-mediated synthesis of mono- and bimetallic silver/gold	0:60	Starch-mediated synthesis of mono- and bimetallic silver/gold nanoparticles as antimicrobial and anticancer agents.
30988615	9	51	theme	growth	1634:1639	arg1	media					1641:1645	the growth media	1630:1645	the growth media of human dermal fibroblast (HDF) and human melanoma cells	1630:1703	In addition, the NPs were added to the growth media of human dermal fibroblast (HDF) and human melanoma cells to show their cytocompatibility and cytotoxicity, respectively, over a 3-day experiment.
30988615	7	52	theme	energy-dispersive	1260:1276	arg1	EDS					1298:1300	EDS	1298:1300	EDS	1298:1300	The chemical composition of the nanomaterials was determined by energy-dispersive X-ray spectroscopy (EDS) and inductively coupled plasma mass spectrometry (ICP-MS) analysis.
30988615	7	52	theme	energy-dispersive	1260:1276	arg1	spectroscopy					1284:1295	energy-dispersive X-ray spectroscopy	1260:1295	energy-dispersive X-ray spectroscopy (EDS)	1260:1301	The chemical composition of the nanomaterials was determined by energy-dispersive X-ray spectroscopy (EDS) and inductively coupled plasma mass spectrometry (ICP-MS) analysis.
30988615	8	53	theme	nanostructures	1435:1448	arg1	applications					1403:1414	the biomedical applications	1388:1414	the biomedical applications of the synthesized nanostructures	1388:1448	To further study the biomedical applications of the synthesized nanostructures, antibacterial studies against multidrug-resistant (MDR) Escherichia coli and methicillin-resistant Staphylococcus aureus (MRSA) were conducted.
30988615	14	54	theme	bimetallic	2445:2454	arg1	NPs					2456:2458	bimetallic NPs	2445:2458	bimetallic NPs	2445:2458	CONCLUSION In this study, mono- and bimetallic NPs were synthesized for the first time using a highly reproducible, environmentally friendly, cost-effective and quick method and were successfully characterized and tested for several anti-infection and anticancer biomedical applications.
30988615	13	55	theme	NP	2377:2378	arg1	concentrations					2380:2393	different NP concentrations	2367:2393	different NP concentrations	2367:2393	Furthermore, the NPs showed cytocompat-ibility towards HDF, but a dose-dependent anticancer effect was found when human melanoma cells were grown in presence of different NP concentrations for 72 hours.
30988615	6	56	theme	electron	1093:1100	arg1	TEM					1114:1116	TEM	1114:1116	TEM	1114:1116	Transmission electron microscopy (TEM) imaging was done to determine the morphology and size of the nanostructures.
30988615	6	56	theme	electron	1093:1100	arg1	microscopy					1102:1111	Transmission electron microscopy	1080:1111	Transmission electron microscopy (TEM) imaging	1080:1125	Transmission electron microscopy (TEM) imaging was done to determine the morphology and size of the nanostructures.
30988615	14	57	theme	reproducible	2511:2522	arg1	method					2576:2581	a highly reproducible, environmentally friendly, cost-effective and quick method	2502:2581	a highly reproducible, environmentally friendly, cost-effective and quick method	2502:2581	CONCLUSION In this study, mono- and bimetallic NPs were synthesized for the first time using a highly reproducible, environmentally friendly, cost-effective and quick method and were successfully characterized and tested for several anti-infection and anticancer biomedical applications.
30988615	9	58	theme	dermal	1656:1661	arg1	HDF					1675:1677	human dermal fibroblast (HDF) and human melanoma cells	1650:1703	HDF	1675:1677	In addition, the NPs were added to the growth media of human dermal fibroblast (HDF) and human melanoma cells to show their cytocompatibility and cytotoxicity, respectively, over a 3-day experiment.
30988615	7	59	theme	nanomaterials	1228:1240	arg1	composition					1209:1219	The chemical composition	1196:1219	The chemical composition of the nanomaterials	1196:1240	The chemical composition of the nanomaterials was determined by energy-dispersive X-ray spectroscopy (EDS) and inductively coupled plasma mass spectrometry (ICP-MS) analysis.
30988615	3	60	theme	optical	698:704	arg1	properties					706:715	The optical properties	694:715	The optical properties	694:715	METHODS The optical properties, high reproducibility, stability and particle size distribution of the colloidal NPs were analyzed by ultraviolet (UV)-visible spectroscopy, dynamic light scattering (DLS) and ζ-potential.
30988615	1	61	theme	gold	320:323	arg1	AuNPs					340:344	AuNPs	340:344	AuNPs	340:344	BACKGROUND AND AIM Bimetallic silver/gold nanosystems are expected to significantly improve therapeutic efficacy compared to their monometallic counterparts by maintaining the general biocompatibility of gold nanoparticles (AuNPs) while, at the same time, decreasing the relatively high toxicity of silver nanoparticles (AgNPs) toward healthy human cells.
30988615	1	61	theme	gold	320:323	arg1	nanoparticles					325:337	gold nanoparticles	320:337	gold nanoparticles (AuNPs)	320:345	BACKGROUND AND AIM Bimetallic silver/gold nanosystems are expected to significantly improve therapeutic efficacy compared to their monometallic counterparts by maintaining the general biocompatibility of gold nanoparticles (AuNPs) while, at the same time, decreasing the relatively high toxicity of silver nanoparticles (AgNPs) toward healthy human cells.
30988615	2	62	theme	alloy	606:610	arg1	NPs					627:629	NPs	627:629	NPs; Ag/AuNPs	627:639	Thus, the aim of this research was to establish a highly reproducible one-pot green synthesis of colloidal AuNPs and bimetallic Ag/Au alloy nanoparticles (NPs; Ag/AuNPs) using starch as reducing and capping agent.
30988615	2	62	theme	alloy	606:610	arg1	nanoparticles					612:624	colloidal AuNPs and bimetallic Ag/Au alloy nanoparticles	569:624	colloidal AuNPs and bimetallic Ag/Au alloy nanoparticles (NPs; Ag/AuNPs) using starch as reducing and capping agent	569:683	Thus, the aim of this research was to establish a highly reproducible one-pot green synthesis of colloidal AuNPs and bimetallic Ag/Au alloy nanoparticles (NPs; Ag/AuNPs) using starch as reducing and capping agent.
30988615	13	63	theme	different	2367:2375	arg1	concentrations					2380:2393	different NP concentrations	2367:2393	different NP concentrations	2367:2393	Furthermore, the NPs showed cytocompat-ibility towards HDF, but a dose-dependent anticancer effect was found when human melanoma cells were grown in presence of different NP concentrations for 72 hours.
30988615	9	64	theme	human	1684:1688	arg1	cells					1699:1703	human dermal fibroblast (HDF) and human melanoma cells	1650:1703	cells	1699:1703	In addition, the NPs were added to the growth media of human dermal fibroblast (HDF) and human melanoma cells to show their cytocompatibility and cytotoxicity, respectively, over a 3-day experiment.
30988615	9	65	theme	cells	1699:1703	arg1	media					1641:1645	the growth media	1630:1645	the growth media of human dermal fibroblast (HDF) and human melanoma cells	1630:1703	In addition, the NPs were added to the growth media of human dermal fibroblast (HDF) and human melanoma cells to show their cytocompatibility and cytotoxicity, respectively, over a 3-day experiment.
30988615	8	66	theme	antibacterial	1451:1463	arg1	studies					1465:1471	antibacterial studies	1451:1471	antibacterial studies against multidrug-resistant (MDR) Escherichia coli and methicillin-resistant Staphylococcus aureus (MRSA)	1451:1577	To further study the biomedical applications of the synthesized nanostructures, antibacterial studies against multidrug-resistant (MDR) Escherichia coli and methicillin-resistant Staphylococcus aureus (MRSA) were conducted.
30988615	10	67	dep	RESULTS	1794:1800	arg1	confirmed					1826:1834	confirmed	1826:1834	confirmed the highly reproducible green synthesis of colloidal AuNPs and Ag/AuNPs	1826:1906	RESULTS UV-visible spectroscopy confirmed the highly reproducible green synthesis of colloidal AuNPs and Ag/AuNPs.
30988615	3	68	theme	particle	754:761	arg1	distribution					768:779	particle size distribution	754:779	particle size distribution	754:779	METHODS The optical properties, high reproducibility, stability and particle size distribution of the colloidal NPs were analyzed by ultraviolet (UV)-visible spectroscopy, dynamic light scattering (DLS) and ζ-potential.
30988615	1	69	theme	nanoparticles	422:434	arg1	toxicity					403:410	the relatively high toxicity	383:410	the relatively high toxicity of silver nanoparticles (AgNPs) toward healthy human cells	383:469	BACKGROUND AND AIM Bimetallic silver/gold nanosystems are expected to significantly improve therapeutic efficacy compared to their monometallic counterparts by maintaining the general biocompatibility of gold nanoparticles (AuNPs) while, at the same time, decreasing the relatively high toxicity of silver nanoparticles (AgNPs) toward healthy human cells.
30988615	1	70	theme	monometallic	247:258	arg1	counterparts					260:271	their monometallic counterparts	241:271	their monometallic counterparts	241:271	BACKGROUND AND AIM Bimetallic silver/gold nanosystems are expected to significantly improve therapeutic efficacy compared to their monometallic counterparts by maintaining the general biocompatibility of gold nanoparticles (AuNPs) while, at the same time, decreasing the relatively high toxicity of silver nanoparticles (AgNPs) toward healthy human cells.
30988615	4	71	theme	starch	922:927	arg1	presence					910:917	The presence	906:917	The presence of starch as capping agent	906:944	The presence of starch as capping agent was determined by Fourier transform infrared (FT-IR) spectroscopy.
30988615	12	72	theme	antibiotic-resistant	2116:2135	arg1	strains					2147:2153	antibiotic-resistant bacterial strains	2116:2153	antibiotic-resistant bacterial strains	2116:2153	The antibacterial studies of the NPs against antibiotic-resistant bacterial strains presented a dose-dependent antimicrobial behavior.
30988615	3	73	theme	NPs	798:800	arg1	properties					706:715	The optical properties	694:715	The optical properties	694:715	METHODS The optical properties, high reproducibility, stability and particle size distribution of the colloidal NPs were analyzed by ultraviolet (UV)-visible spectroscopy, dynamic light scattering (DLS) and ζ-potential.
30988615	3	73	theme	NPs	798:800	arg1	reproducibility					723:737	high reproducibility	718:737	high reproducibility	718:737	METHODS The optical properties, high reproducibility, stability and particle size distribution of the colloidal NPs were analyzed by ultraviolet (UV)-visible spectroscopy, dynamic light scattering (DLS) and ζ-potential.
30988615	3	73	theme	NPs	798:800	arg1	distribution					768:779	particle size distribution	754:779	particle size distribution	754:779	METHODS The optical properties, high reproducibility, stability and particle size distribution of the colloidal NPs were analyzed by ultraviolet (UV)-visible spectroscopy, dynamic light scattering (DLS) and ζ-potential.
30988615	3	73	theme	NPs	798:800	arg1	stability					740:748	stability	740:748	stability	740:748	METHODS The optical properties, high reproducibility, stability and particle size distribution of the colloidal NPs were analyzed by ultraviolet (UV)-visible spectroscopy, dynamic light scattering (DLS) and ζ-potential.
30988615	10	74	theme	UV-visible	1802:1811	arg1	spectroscopy					1813:1824	UV-visible spectroscopy	1802:1824	UV-visible spectroscopy	1802:1824	RESULTS UV-visible spectroscopy confirmed the highly reproducible green synthesis of colloidal AuNPs and Ag/AuNPs.
30988615	13	75	theme	human	2320:2324	arg1	cells					2335:2339	human melanoma cells	2320:2339	human melanoma cells	2320:2339	Furthermore, the NPs showed cytocompat-ibility towards HDF, but a dose-dependent anticancer effect was found when human melanoma cells were grown in presence of different NP concentrations for 72 hours.
30988615	14	76	theme	biomedical	2672:2681	arg1	applications					2683:2694	several anti-infection and anticancer biomedical applications	2634:2694	several anti-infection and anticancer biomedical applications	2634:2694	CONCLUSION In this study, mono- and bimetallic NPs were synthesized for the first time using a highly reproducible, environmentally friendly, cost-effective and quick method and were successfully characterized and tested for several anti-infection and anticancer biomedical applications.
30988615	2	77	theme	colloidal	569:577	arg1	AuNPs					579:583	colloidal AuNPs	569:583	colloidal AuNPs	569:583	Thus, the aim of this research was to establish a highly reproducible one-pot green synthesis of colloidal AuNPs and bimetallic Ag/Au alloy nanoparticles (NPs; Ag/AuNPs) using starch as reducing and capping agent.
30988615	12	78	theme	antibacterial	2075:2087	arg1	studies					2089:2095	The antibacterial studies	2071:2095	The antibacterial studies of the NPs against antibiotic-resistant bacterial strains	2071:2153	The antibacterial studies of the NPs against antibiotic-resistant bacterial strains presented a dose-dependent antimicrobial behavior.
30988615	4	79	dep	Fourier	964:970	arg1	transform					972:980	transform	972:980	transform infrared (FT-IR) spectroscopy	972:1010	The presence of starch as capping agent was determined by Fourier transform infrared (FT-IR) spectroscopy.
30988615	10	80	theme	green	1860:1864	arg1	synthesis					1866:1874	the highly reproducible green synthesis	1836:1874	the highly reproducible green synthesis of colloidal AuNPs and Ag/AuNPs	1836:1906	RESULTS UV-visible spectroscopy confirmed the highly reproducible green synthesis of colloidal AuNPs and Ag/AuNPs.
30988615	1	81	theme	silver/gold	146:156	arg1	nanosystems					158:168	Bimetallic silver/gold nanosystems	135:168	Bimetallic silver/gold nanosystems	135:168	BACKGROUND AND AIM Bimetallic silver/gold nanosystems are expected to significantly improve therapeutic efficacy compared to their monometallic counterparts by maintaining the general biocompatibility of gold nanoparticles (AuNPs) while, at the same time, decreasing the relatively high toxicity of silver nanoparticles (AgNPs) toward healthy human cells.
30988615	3	82	theme	light	866:870	arg1	DLS					884:886	DLS	884:886	DLS	884:886	METHODS The optical properties, high reproducibility, stability and particle size distribution of the colloidal NPs were analyzed by ultraviolet (UV)-visible spectroscopy, dynamic light scattering (DLS) and ζ-potential.
30988615	3	82	theme	light	866:870	arg1	scattering					872:881	dynamic light scattering	858:881	dynamic light scattering (DLS)	858:887	METHODS The optical properties, high reproducibility, stability and particle size distribution of the colloidal NPs were analyzed by ultraviolet (UV)-visible spectroscopy, dynamic light scattering (DLS) and ζ-potential.
30988615	2	83	theme	AuNPs	579:583	arg1	NPs					627:629	NPs	627:629	NPs; Ag/AuNPs	627:639	Thus, the aim of this research was to establish a highly reproducible one-pot green synthesis of colloidal AuNPs and bimetallic Ag/Au alloy nanoparticles (NPs; Ag/AuNPs) using starch as reducing and capping agent.
30988615	2	83	theme	AuNPs	579:583	arg1	nanoparticles					612:624	colloidal AuNPs and bimetallic Ag/Au alloy nanoparticles	569:624	colloidal AuNPs and bimetallic Ag/Au alloy nanoparticles (NPs; Ag/AuNPs) using starch as reducing and capping agent	569:683	Thus, the aim of this research was to establish a highly reproducible one-pot green synthesis of colloidal AuNPs and bimetallic Ag/Au alloy nanoparticles (NPs; Ag/AuNPs) using starch as reducing and capping agent.
30988615	6	84	theme	nanostructures	1180:1193	arg1	morphology					1153:1162	morphology	1153:1162	morphology	1153:1162	Transmission electron microscopy (TEM) imaging was done to determine the morphology and size of the nanostructures.
30988615	6	84	theme	nanostructures	1180:1193	arg1	size					1168:1171	size	1168:1171	size	1168:1171	Transmission electron microscopy (TEM) imaging was done to determine the morphology and size of the nanostructures.
30988615	3	85	theme	ultraviolet	819:829	arg1	spectroscopy					844:855	ultraviolet (UV)-visible spectroscopy	819:855	ultraviolet (UV)-visible spectroscopy	819:855	METHODS The optical properties, high reproducibility, stability and particle size distribution of the colloidal NPs were analyzed by ultraviolet (UV)-visible spectroscopy, dynamic light scattering (DLS) and ζ-potential.
30988615	0	86	theme	antimicrobial	79:91	arg1	agents					108:113	antimicrobial and anticancer agents	79:113	agents	108:113	Starch-mediated synthesis of mono- and bimetallic silver/gold nanoparticles as antimicrobial and anticancer agents.
30988615	5	87	theme	X-ray	1055:1059	arg1	XRD					1074:1076	XRD	1074:1076	XRD	1074:1076	The structural properties were studied by X-ray diffraction (XRD).
30988615	5	87	theme	X-ray	1055:1059	arg1	diffraction					1061:1071	X-ray diffraction	1055:1071	X-ray diffraction (XRD)	1055:1077	The structural properties were studied by X-ray diffraction (XRD).
30988615	11	88	theme	mean	1994:1997	arg1	sizes					2008:2012	mean particle sizes	1994:2012	mean particle sizes of 28.5 and 9.7 nm for AuNPs and Ag/AuNPs, respectively	1994:2068	The NPs showed a face-centered cubic crystal structure and an icosahedral shape with mean particle sizes of 28.5 and 9.7 nm for AuNPs and Ag/AuNPs, respectively.
30988615	14	89	theme	several	2634:2640	arg1	applications					2683:2694	several anti-infection and anticancer biomedical applications	2634:2694	several anti-infection and anticancer biomedical applications	2634:2694	CONCLUSION In this study, mono- and bimetallic NPs were synthesized for the first time using a highly reproducible, environmentally friendly, cost-effective and quick method and were successfully characterized and tested for several anti-infection and anticancer biomedical applications.
30988615	9	90	theme	3-day	1776:1780	arg1	experiment					1782:1791	a 3-day experiment	1774:1791	a 3-day experiment	1774:1791	In addition, the NPs were added to the growth media of human dermal fibroblast (HDF) and human melanoma cells to show their cytocompatibility and cytotoxicity, respectively, over a 3-day experiment.
30988615	0	91	theme	anticancer	97:106	arg1	agents					108:113	antimicrobial and anticancer agents	79:113	agents	108:113	Starch-mediated synthesis of mono- and bimetallic silver/gold nanoparticles as antimicrobial and anticancer agents.
30988615	2	92	theme	research	494:501	arg1	aim					482:484	the aim	478:484	the aim of this research	478:501	Thus, the aim of this research was to establish a highly reproducible one-pot green synthesis of colloidal AuNPs and bimetallic Ag/Au alloy nanoparticles (NPs; Ag/AuNPs) using starch as reducing and capping agent.
30988615	3	93	theme	-visible	835:842	arg1	spectroscopy					844:855	ultraviolet (UV)-visible spectroscopy	819:855	ultraviolet (UV)-visible spectroscopy	819:855	METHODS The optical properties, high reproducibility, stability and particle size distribution of the colloidal NPs were analyzed by ultraviolet (UV)-visible spectroscopy, dynamic light scattering (DLS) and ζ-potential.
30988615	2	94	theme	reproducible	529:540	arg1	synthesis					556:564	a highly reproducible one-pot green synthesis	520:564	a highly reproducible one-pot green synthesis of colloidal AuNPs and bimetallic Ag/Au alloy nanoparticles (NPs; Ag/AuNPs) using starch as reducing and capping agent	520:683	Thus, the aim of this research was to establish a highly reproducible one-pot green synthesis of colloidal AuNPs and bimetallic Ag/Au alloy nanoparticles (NPs; Ag/AuNPs) using starch as reducing and capping agent.
30988615	3	95	theme	UV	832:833	arg1	spectroscopy					844:855	ultraviolet (UV)-visible spectroscopy	819:855	ultraviolet (UV)-visible spectroscopy	819:855	METHODS The optical properties, high reproducibility, stability and particle size distribution of the colloidal NPs were analyzed by ultraviolet (UV)-visible spectroscopy, dynamic light scattering (DLS) and ζ-potential.
30988615	1	96	theme	high	398:401	arg1	toxicity					403:410	the relatively high toxicity	383:410	the relatively high toxicity of silver nanoparticles (AgNPs) toward healthy human cells	383:469	BACKGROUND AND AIM Bimetallic silver/gold nanosystems are expected to significantly improve therapeutic efficacy compared to their monometallic counterparts by maintaining the general biocompatibility of gold nanoparticles (AuNPs) while, at the same time, decreasing the relatively high toxicity of silver nanoparticles (AgNPs) toward healthy human cells.
30988615	12	97	theme	NPs	2104:2106	arg1	studies					2089:2095	The antibacterial studies	2071:2095	The antibacterial studies of the NPs against antibiotic-resistant bacterial strains	2071:2153	The antibacterial studies of the NPs against antibiotic-resistant bacterial strains presented a dose-dependent antimicrobial behavior.
30988615	7	98	theme	plasma	1327:1332	arg1	ICP-MS					1353:1358	ICP-MS	1353:1358	ICP-MS	1353:1358	The chemical composition of the nanomaterials was determined by energy-dispersive X-ray spectroscopy (EDS) and inductively coupled plasma mass spectrometry (ICP-MS) analysis.
30988615	7	98	theme	plasma	1327:1332	arg1	spectrometry					1339:1350	plasma mass spectrometry	1327:1350	inductively coupled plasma mass spectrometry (ICP-MS) analysis	1307:1368	The chemical composition of the nanomaterials was determined by energy-dispersive X-ray spectroscopy (EDS) and inductively coupled plasma mass spectrometry (ICP-MS) analysis.
30988615	11	99	theme	face-centered	1926:1938	arg1	structure					1954:1962	a face-centered cubic crystal structure	1924:1962	a face-centered cubic crystal structure	1924:1962	The NPs showed a face-centered cubic crystal structure and an icosahedral shape with mean particle sizes of 28.5 and 9.7 nm for AuNPs and Ag/AuNPs, respectively.
30988615	0	100	theme	mono-	29:33	arg1	silver/gold					50:60	mono- and bimetallic silver/gold	29:60	mono- and bimetallic silver/gold	29:60	Starch-mediated synthesis of mono- and bimetallic silver/gold nanoparticles as antimicrobial and anticancer agents.
30988615	10	101	theme	AuNPs	1889:1893	arg1	synthesis					1866:1874	the highly reproducible green synthesis	1836:1874	the highly reproducible green synthesis of colloidal AuNPs and Ag/AuNPs	1836:1906	RESULTS UV-visible spectroscopy confirmed the highly reproducible green synthesis of colloidal AuNPs and Ag/AuNPs.
30988615	0	102	theme	bimetallic	39:48	arg1	silver/gold					50:60	mono- and bimetallic silver/gold	29:60	mono- and bimetallic silver/gold	29:60	Starch-mediated synthesis of mono- and bimetallic silver/gold nanoparticles as antimicrobial and anticancer agents.
30988615	8	103	dep	multidrug-resistant	1481:1499	arg1	MDR					1502:1504	MDR	1502:1504	MDR	1502:1504	To further study the biomedical applications of the synthesized nanostructures, antibacterial studies against multidrug-resistant (MDR) Escherichia coli and methicillin-resistant Staphylococcus aureus (MRSA) were conducted.
30988615	11	104	theme	crystal	1946:1952	arg1	structure					1954:1962	a face-centered cubic crystal structure	1924:1962	a face-centered cubic crystal structure	1924:1962	The NPs showed a face-centered cubic crystal structure and an icosahedral shape with mean particle sizes of 28.5 and 9.7 nm for AuNPs and Ag/AuNPs, respectively.
30988615	14	105	theme	quick	2570:2574	arg1	method					2576:2581	a highly reproducible, environmentally friendly, cost-effective and quick method	2502:2581	a highly reproducible, environmentally friendly, cost-effective and quick method	2502:2581	CONCLUSION In this study, mono- and bimetallic NPs were synthesized for the first time using a highly reproducible, environmentally friendly, cost-effective and quick method and were successfully characterized and tested for several anti-infection and anticancer biomedical applications.
30988615	5	106	theme	structural	1017:1026	arg1	properties					1028:1037	The structural properties	1013:1037	The structural properties	1013:1037	The structural properties were studied by X-ray diffraction (XRD).
30988615	7	107	theme	X-ray	1278:1282	arg1	EDS					1298:1300	EDS	1298:1300	EDS	1298:1300	The chemical composition of the nanomaterials was determined by energy-dispersive X-ray spectroscopy (EDS) and inductively coupled plasma mass spectrometry (ICP-MS) analysis.
30988615	7	107	theme	X-ray	1278:1282	arg1	spectroscopy					1284:1295	energy-dispersive X-ray spectroscopy	1260:1295	energy-dispersive X-ray spectroscopy (EDS)	1260:1301	The chemical composition of the nanomaterials was determined by energy-dispersive X-ray spectroscopy (EDS) and inductively coupled plasma mass spectrometry (ICP-MS) analysis.
30988615	2	108	theme	green	550:554	arg1	synthesis					556:564	a highly reproducible one-pot green synthesis	520:564	a highly reproducible one-pot green synthesis of colloidal AuNPs and bimetallic Ag/Au alloy nanoparticles (NPs; Ag/AuNPs) using starch as reducing and capping agent	520:683	Thus, the aim of this research was to establish a highly reproducible one-pot green synthesis of colloidal AuNPs and bimetallic Ag/Au alloy nanoparticles (NPs; Ag/AuNPs) using starch as reducing and capping agent.
30988615	8	109	theme	biomedical	1392:1401	arg1	applications					1403:1414	the biomedical applications	1388:1414	the biomedical applications of the synthesized nanostructures	1388:1448	To further study the biomedical applications of the synthesized nanostructures, antibacterial studies against multidrug-resistant (MDR) Escherichia coli and methicillin-resistant Staphylococcus aureus (MRSA) were conducted.
30988615	1	110	theme	same	361:364	arg1	time					366:369	the same time	357:369	the same time	357:369	BACKGROUND AND AIM Bimetallic silver/gold nanosystems are expected to significantly improve therapeutic efficacy compared to their monometallic counterparts by maintaining the general biocompatibility of gold nanoparticles (AuNPs) while, at the same time, decreasing the relatively high toxicity of silver nanoparticles (AgNPs) toward healthy human cells.
30988615	14	111	theme	cost-effective	2551:2564	arg1	method					2576:2581	a highly reproducible, environmentally friendly, cost-effective and quick method	2502:2581	a highly reproducible, environmentally friendly, cost-effective and quick method	2502:2581	CONCLUSION In this study, mono- and bimetallic NPs were synthesized for the first time using a highly reproducible, environmentally friendly, cost-effective and quick method and were successfully characterized and tested for several anti-infection and anticancer biomedical applications.
30988615	10	112	theme	Ag/AuNPs	1899:1906	arg1	synthesis					1866:1874	the highly reproducible green synthesis	1836:1874	the highly reproducible green synthesis of colloidal AuNPs and Ag/AuNPs	1836:1906	RESULTS UV-visible spectroscopy confirmed the highly reproducible green synthesis of colloidal AuNPs and Ag/AuNPs.
30988615	12	113	theme	antimicrobial	2182:2194	arg1	behavior					2196:2203	a dose-dependent antimicrobial behavior	2165:2203	a dose-dependent antimicrobial behavior	2165:2203	The antibacterial studies of the NPs against antibiotic-resistant bacterial strains presented a dose-dependent antimicrobial behavior.
30988615	14	114	theme	friendly	2541:2548	arg1	method					2576:2581	a highly reproducible, environmentally friendly, cost-effective and quick method	2502:2581	a highly reproducible, environmentally friendly, cost-effective and quick method	2502:2581	CONCLUSION In this study, mono- and bimetallic NPs were synthesized for the first time using a highly reproducible, environmentally friendly, cost-effective and quick method and were successfully characterized and tested for several anti-infection and anticancer biomedical applications.
30988615	6	115	theme	microscopy	1102:1111	arg1	imaging					1119:1125	Transmission electron microscopy (TEM) imaging	1080:1125	Transmission electron microscopy (TEM) imaging	1080:1125	Transmission electron microscopy (TEM) imaging was done to determine the morphology and size of the nanostructures.
30988615	8	116	theme	synthesized	1423:1433	arg1	nanostructures					1435:1448	the synthesized nanostructures	1419:1448	the synthesized nanostructures	1419:1448	To further study the biomedical applications of the synthesized nanostructures, antibacterial studies against multidrug-resistant (MDR) Escherichia coli and methicillin-resistant Staphylococcus aureus (MRSA) were conducted.
30988615	1	117	theme	general	292:298	arg1	biocompatibility					300:315	the general biocompatibility	288:315	the general biocompatibility of gold nanoparticles (AuNPs)	288:345	BACKGROUND AND AIM Bimetallic silver/gold nanosystems are expected to significantly improve therapeutic efficacy compared to their monometallic counterparts by maintaining the general biocompatibility of gold nanoparticles (AuNPs) while, at the same time, decreasing the relatively high toxicity of silver nanoparticles (AgNPs) toward healthy human cells.
28961797	11	0	from	increase	1390:1397	arg1	[ATG1/ULK-1					1417:1427	macroautophagy [ATG1/ULK-1	1402:1427	macroautophagy [ATG1/ULK-1	1402:1427	We observed an increase in macroautophagy [ATG1/ULK-1, ATG13, ATG5, ATG16L1, and MAP1LC3-I/II] and in CMA [HSPA8 and HSP90AA1], whereas there was a decrease in the UPR [PERK, IRE-1α, and ATF-6α] protein expression levels in TREM-1 inhibited colitic mice.
28961797	11	0	from	increase	1390:1397	arg1	[HSPA8					1481:1486	CMA [HSPA8	1477:1486	CMA [HSPA8	1477:1486	We observed an increase in macroautophagy [ATG1/ULK-1, ATG13, ATG5, ATG16L1, and MAP1LC3-I/II] and in CMA [HSPA8 and HSP90AA1], whereas there was a decrease in the UPR [PERK, IRE-1α, and ATF-6α] protein expression levels in TREM-1 inhibited colitic mice.
28961797	11	0	from	increase	1390:1397	arg1	HSP90AA1					1492:1499	HSP90AA1	1492:1499	HSP90AA1	1492:1499	We observed an increase in macroautophagy [ATG1/ULK-1, ATG13, ATG5, ATG16L1, and MAP1LC3-I/II] and in CMA [HSPA8 and HSP90AA1], whereas there was a decrease in the UPR [PERK, IRE-1α, and ATF-6α] protein expression levels in TREM-1 inhibited colitic mice.
28961797	8	1	theme	autophagy	1061:1069	arg1	Levels					1016:1021	Levels	1016:1021	Levels of macroautophagy, chaperone-mediated autophagy [CMA], and UPR proteins	1016:1093	Levels of macroautophagy, chaperone-mediated autophagy [CMA], and UPR proteins were evaluated by western blotting.
28961797	9	2	theme	MiSeq	1192:1196	arg1	sequencing					1198:1207	MiSeq sequencing	1192:1207	MiSeq sequencing	1192:1207	The composition of the intestinal microbiota was assessed by MiSeq sequencing in both LR12-treated and KO animals.
28961797	3	3	theme	host-microbiota	480:494	arg1	interactions					496:507	host-microbiota interactions	480:507	host-microbiota interactions for the clearance of intracellular pathogens	480:552	Both autophagy and UPR are deeply involved in host-microbiota interactions for the clearance of intracellular pathogens, thus contributing to dysbiosis.
28961797	10	4	theme	TREM-1	1286:1291	arg1	inhibition					1272:1281	inhibition	1272:1281	inhibition of TREM-1	1272:1291	RESULTS We confirmed that inhibition of TREM-1 attenuates the severity of colitis clinically, endoscopically and histologically.
28961797	11	5	from	ATF-6α	1562:1567	arg1	[PERK					1543:1547	the UPR [PERK	1535:1547	the UPR [PERK	1535:1547	We observed an increase in macroautophagy [ATG1/ULK-1, ATG13, ATG5, ATG16L1, and MAP1LC3-I/II] and in CMA [HSPA8 and HSP90AA1], whereas there was a decrease in the UPR [PERK, IRE-1α, and ATF-6α] protein expression levels in TREM-1 inhibited colitic mice.
28961797	11	5	from	ATF-6α	1562:1567	arg1	mice					1624:1627	TREM-1 inhibited colitic mice	1599:1627	TREM-1 inhibited colitic mice	1599:1627	We observed an increase in macroautophagy [ATG1/ULK-1, ATG13, ATG5, ATG16L1, and MAP1LC3-I/II] and in CMA [HSPA8 and HSP90AA1], whereas there was a decrease in the UPR [PERK, IRE-1α, and ATF-6α] protein expression levels in TREM-1 inhibited colitic mice.
28961797	11	6	theme	colitic	1616:1622	arg1	mice					1624:1627	TREM-1 inhibited colitic mice	1599:1627	TREM-1 inhibited colitic mice	1599:1627	We observed an increase in macroautophagy [ATG1/ULK-1, ATG13, ATG5, ATG16L1, and MAP1LC3-I/II] and in CMA [HSPA8 and HSP90AA1], whereas there was a decrease in the UPR [PERK, IRE-1α, and ATF-6α] protein expression levels in TREM-1 inhibited colitic mice.
28961797	8	7	theme	chaperone-mediated	1042:1059	arg1	autophagy					1061:1069	chaperone-mediated autophagy [CMA]	1042:1075	chaperone-mediated autophagy [CMA]	1042:1075	Levels of macroautophagy, chaperone-mediated autophagy [CMA], and UPR proteins were evaluated by western blotting.
28961797	11	8	theme	UPR	1539:1541	arg1	[PERK					1543:1547	the UPR [PERK	1535:1547	the UPR [PERK	1535:1547	We observed an increase in macroautophagy [ATG1/ULK-1, ATG13, ATG5, ATG16L1, and MAP1LC3-I/II] and in CMA [HSPA8 and HSP90AA1], whereas there was a decrease in the UPR [PERK, IRE-1α, and ATF-6α] protein expression levels in TREM-1 inhibited colitic mice.
28961797	6	9	theme	LR12	883:886	arg1	peptide					888:894	LR12 peptide	883:894	LR12 peptide	883:894	TREM-1 was inhibited, either pharmacologically by LR12 peptide or genetically with Trem-1 knock-out [KO] mice.
28961797	5	10	theme	experimental	743:754	arg1	model					762:766	An experimental mouse model	740:766	METHODS An experimental mouse model of colitis	732:777	METHODS An experimental mouse model of colitis was established by dextran sulphate sodium treatment.
28961797	9	11	theme	KO	1234:1235	arg1	animals					1237:1243	both LR12-treated and KO animals	1212:1243	both LR12-treated and KO animals	1212:1243	The composition of the intestinal microbiota was assessed by MiSeq sequencing in both LR12-treated and KO animals.
28961797	13	12	theme	drug	1710:1713	arg1	target					1715:1720	a novel drug target	1702:1720	a novel drug target for the treatment of IBD	1702:1745	CONCLUSIONS TREM-1 may represent a novel drug target for the treatment of IBD, by modulating autophagy activity and ER stress.
28961797	7	13	theme	colitic	992:998	arg1	mice					1000:1003	control and colitic mice	980:1003	control and colitic mice	980:1003	Colon tissues and faecal pellets of control and colitic mice were used.
28961797	2	14	theme	unfolded	284:291	arg1	[UPR					310:313	the unfolded protein response [UPR	280:313	the unfolded protein response [UPR	280:313	Autophagy and endoplasmic reticulum [ER] stress, which activate the unfolded protein response [UPR], are closely linked and defects in these pathways contribute to the pathogenesis of inflammatory bowel disease [IBD].
28961797	2	15	theme	protein	293:299	arg1	[UPR					310:313	the unfolded protein response [UPR	280:313	the unfolded protein response [UPR	280:313	Autophagy and endoplasmic reticulum [ER] stress, which activate the unfolded protein response [UPR], are closely linked and defects in these pathways contribute to the pathogenesis of inflammatory bowel disease [IBD].
28961797	5	16	theme	mouse	756:760	arg1	model					762:766	An experimental mouse model	740:766	METHODS An experimental mouse model of colitis	732:777	METHODS An experimental mouse model of colitis was established by dextran sulphate sodium treatment.
28961797	6	17	theme	[KO	933:935	arg1	mice					938:941	Trem-1 knock-out [KO] mice	916:941	Trem-1 knock-out [KO] mice	916:941	TREM-1 was inhibited, either pharmacologically by LR12 peptide or genetically with Trem-1 knock-out [KO] mice.
28961797	11	18	theme	expression	1578:1587	arg1	levels					1589:1594	protein expression levels	1570:1594	protein expression levels	1570:1594	We observed an increase in macroautophagy [ATG1/ULK-1, ATG13, ATG5, ATG16L1, and MAP1LC3-I/II] and in CMA [HSPA8 and HSP90AA1], whereas there was a decrease in the UPR [PERK, IRE-1α, and ATF-6α] protein expression levels in TREM-1 inhibited colitic mice.
28961797	3	19	theme	pathogens	544:552	arg1	clearance					517:525	the clearance	513:525	the clearance of intracellular pathogens	513:552	Both autophagy and UPR are deeply involved in host-microbiota interactions for the clearance of intracellular pathogens, thus contributing to dysbiosis.
28961797	10	20	theme	colitis	1320:1326	arg1	severity					1308:1315	the severity	1304:1315	the severity of colitis	1304:1326	RESULTS We confirmed that inhibition of TREM-1 attenuates the severity of colitis clinically, endoscopically and histologically.
28961797	6	21	theme	knock-out	923:931	arg1	mice					938:941	Trem-1 knock-out [KO] mice	916:941	Trem-1 knock-out [KO] mice	916:941	TREM-1 was inhibited, either pharmacologically by LR12 peptide or genetically with Trem-1 knock-out [KO] mice.
28961797	13	22	theme	CONCLUSIONS	1669:1679	arg1	TREM-1					1681:1686	CONCLUSIONS TREM-1	1669:1686	CONCLUSIONS TREM-1	1669:1686	CONCLUSIONS TREM-1 may represent a novel drug target for the treatment of IBD, by modulating autophagy activity and ER stress.
28961797	2	23	theme	inflammatory	400:411	arg1	disease					419:425	inflammatory bowel disease	400:425	inflammatory bowel disease [IBD]	400:431	Autophagy and endoplasmic reticulum [ER] stress, which activate the unfolded protein response [UPR], are closely linked and defects in these pathways contribute to the pathogenesis of inflammatory bowel disease [IBD].
28961797	11	24	from	levels	1589:1594	arg1	[PERK					1543:1547	the UPR [PERK	1535:1547	the UPR [PERK	1535:1547	We observed an increase in macroautophagy [ATG1/ULK-1, ATG13, ATG5, ATG16L1, and MAP1LC3-I/II] and in CMA [HSPA8 and HSP90AA1], whereas there was a decrease in the UPR [PERK, IRE-1α, and ATF-6α] protein expression levels in TREM-1 inhibited colitic mice.
28961797	11	24	from	levels	1589:1594	arg1	mice					1624:1627	TREM-1 inhibited colitic mice	1599:1627	TREM-1 inhibited colitic mice	1599:1627	We observed an increase in macroautophagy [ATG1/ULK-1, ATG13, ATG5, ATG16L1, and MAP1LC3-I/II] and in CMA [HSPA8 and HSP90AA1], whereas there was a decrease in the UPR [PERK, IRE-1α, and ATF-6α] protein expression levels in TREM-1 inhibited colitic mice.
28961797	13	25	theme	ER	1785:1786	arg1	stress					1788:1793	ER stress	1785:1793	ER stress	1785:1793	CONCLUSIONS TREM-1 may represent a novel drug target for the treatment of IBD, by modulating autophagy activity and ER stress.
28961797	6	26	theme	Trem-1	916:921	arg1	mice					938:941	Trem-1 knock-out [KO] mice	916:941	Trem-1 knock-out [KO] mice	916:941	TREM-1 was inhibited, either pharmacologically by LR12 peptide or genetically with Trem-1 knock-out [KO] mice.
28961797	2	27	dep	disease	419:425	arg1	[IBD					427:430	[IBD	427:430	[IBD	427:430	Autophagy and endoplasmic reticulum [ER] stress, which activate the unfolded protein response [UPR], are closely linked and defects in these pathways contribute to the pathogenesis of inflammatory bowel disease [IBD].
28961797	2	28	from	defects	340:346	arg1	pathways					357:364	these pathways	351:364	these pathways	351:364	Autophagy and endoplasmic reticulum [ER] stress, which activate the unfolded protein response [UPR], are closely linked and defects in these pathways contribute to the pathogenesis of inflammatory bowel disease [IBD].
28961797	11	29	theme	inhibited	1606:1614	arg1	mice					1624:1627	TREM-1 inhibited colitic mice	1599:1627	TREM-1 inhibited colitic mice	1599:1627	We observed an increase in macroautophagy [ATG1/ULK-1, ATG13, ATG5, ATG16L1, and MAP1LC3-I/II] and in CMA [HSPA8 and HSP90AA1], whereas there was a decrease in the UPR [PERK, IRE-1α, and ATF-6α] protein expression levels in TREM-1 inhibited colitic mice.
28961797	13	30	theme	IBD	1743:1745	arg1	treatment					1730:1738	the treatment	1726:1738	the treatment of IBD	1726:1745	CONCLUSIONS TREM-1 may represent a novel drug target for the treatment of IBD, by modulating autophagy activity and ER stress.
28961797	8	31	theme	proteins	1086:1093	arg1	Levels					1016:1021	Levels	1016:1021	Levels of macroautophagy, chaperone-mediated autophagy [CMA], and UPR proteins	1016:1093	Levels of macroautophagy, chaperone-mediated autophagy [CMA], and UPR proteins were evaluated by western blotting.
28961797	5	32	dep	METHODS	732:738	arg1	model					762:766	An experimental mouse model	740:766	METHODS An experimental mouse model of colitis	732:777	METHODS An experimental mouse model of colitis was established by dextran sulphate sodium treatment.
28961797	0	33	theme	TREM-1	0:5	arg1	Inhibition					7:16	TREM-1 Inhibition	0:16	TREM-1 Inhibition	0:16	TREM-1 Inhibition Restores Impaired Autophagy Activity and Reduces Colitis in Mice.
28961797	8	34	theme	UPR	1082:1084	arg1	proteins					1086:1093	UPR proteins	1082:1093	UPR proteins	1082:1093	Levels of macroautophagy, chaperone-mediated autophagy [CMA], and UPR proteins were evaluated by western blotting.
28961797	13	35	theme	novel	1704:1708	arg1	target					1715:1720	a novel drug target	1702:1720	a novel drug target for the treatment of IBD	1702:1745	CONCLUSIONS TREM-1 may represent a novel drug target for the treatment of IBD, by modulating autophagy activity and ER stress.
28961797	0	36	theme	Impaired	27:34	arg1	Activity					46:53	Impaired Autophagy Activity	27:53	Impaired Autophagy Activity	27:53	TREM-1 Inhibition Restores Impaired Autophagy Activity and Reduces Colitis in Mice.
28961797	7	37	theme	control	980:986	arg1	mice					1000:1003	control and colitic mice	980:1003	control and colitic mice	980:1003	Colon tissues and faecal pellets of control and colitic mice were used.
28961797	2	38	theme	response	301:308	arg1	[UPR					310:313	the unfolded protein response [UPR	280:313	the unfolded protein response [UPR	280:313	Autophagy and endoplasmic reticulum [ER] stress, which activate the unfolded protein response [UPR], are closely linked and defects in these pathways contribute to the pathogenesis of inflammatory bowel disease [IBD].
28961797	2	39	theme	bowel	413:417	arg1	disease					419:425	inflammatory bowel disease	400:425	inflammatory bowel disease [IBD]	400:431	Autophagy and endoplasmic reticulum [ER] stress, which activate the unfolded protein response [UPR], are closely linked and defects in these pathways contribute to the pathogenesis of inflammatory bowel disease [IBD].
28961797	2	40	theme	disease	419:425	arg1	pathogenesis					384:395	the pathogenesis	380:395	the pathogenesis of inflammatory bowel disease [IBD]	380:431	Autophagy and endoplasmic reticulum [ER] stress, which activate the unfolded protein response [UPR], are closely linked and defects in these pathways contribute to the pathogenesis of inflammatory bowel disease [IBD].
28961797	1	41	theme	Triggering	104:113	arg1	receptor					115:122	Triggering receptor	104:122	Triggering receptor expressed on myeloid cells-1	104:151	BACKGROUND AND AIMS Triggering receptor expressed on myeloid cells-1 [TREM-1] is known to amplify inflammation in several diseases.
28961797	12	42	theme	TREM-1	1630:1635	arg1	inhibition					1637:1646	TREM-1 inhibition	1630:1646	TREM-1 inhibition	1630:1646	TREM-1 inhibition prevented dysbiosis.
28961797	1	43	theme	several	198:204	arg1	diseases					206:213	several diseases	198:213	several diseases	198:213	BACKGROUND AND AIMS Triggering receptor expressed on myeloid cells-1 [TREM-1] is known to amplify inflammation in several diseases.
28961797	5	44	theme	dextran	798:804	arg1	treatment					822:830	dextran sulphate sodium treatment	798:830	dextran sulphate sodium treatment	798:830	METHODS An experimental mouse model of colitis was established by dextran sulphate sodium treatment.
28961797	8	45	theme	western	1113:1119	arg1	blotting					1121:1128	western blotting	1113:1128	western blotting	1113:1128	Levels of macroautophagy, chaperone-mediated autophagy [CMA], and UPR proteins were evaluated by western blotting.
28961797	2	46	theme	reticulum	242:250	arg1	stress					257:262	endoplasmic reticulum [ER] stress	230:262	endoplasmic reticulum [ER] stress	230:262	Autophagy and endoplasmic reticulum [ER] stress, which activate the unfolded protein response [UPR], are closely linked and defects in these pathways contribute to the pathogenesis of inflammatory bowel disease [IBD].
28961797	11	47	theme	CMA	1477:1479	arg1	[HSPA8					1481:1486	CMA [HSPA8	1477:1486	CMA [HSPA8	1477:1486	We observed an increase in macroautophagy [ATG1/ULK-1, ATG13, ATG5, ATG16L1, and MAP1LC3-I/II] and in CMA [HSPA8 and HSP90AA1], whereas there was a decrease in the UPR [PERK, IRE-1α, and ATF-6α] protein expression levels in TREM-1 inhibited colitic mice.
28961797	0	48	theme	Autophagy	36:44	arg1	Activity					46:53	Impaired Autophagy Activity	27:53	Impaired Autophagy Activity	27:53	TREM-1 Inhibition Restores Impaired Autophagy Activity and Reduces Colitis in Mice.
28961797	5	49	theme	sulphate	806:813	arg1	treatment					822:830	dextran sulphate sodium treatment	798:830	dextran sulphate sodium treatment	798:830	METHODS An experimental mouse model of colitis was established by dextran sulphate sodium treatment.
28961797	11	50	from	decrease	1523:1530	arg1	[PERK					1543:1547	the UPR [PERK	1535:1547	the UPR [PERK	1535:1547	We observed an increase in macroautophagy [ATG1/ULK-1, ATG13, ATG5, ATG16L1, and MAP1LC3-I/II] and in CMA [HSPA8 and HSP90AA1], whereas there was a decrease in the UPR [PERK, IRE-1α, and ATF-6α] protein expression levels in TREM-1 inhibited colitic mice.
28961797	11	50	from	decrease	1523:1530	arg1	mice					1624:1627	TREM-1 inhibited colitic mice	1599:1627	TREM-1 inhibited colitic mice	1599:1627	We observed an increase in macroautophagy [ATG1/ULK-1, ATG13, ATG5, ATG16L1, and MAP1LC3-I/II] and in CMA [HSPA8 and HSP90AA1], whereas there was a decrease in the UPR [PERK, IRE-1α, and ATF-6α] protein expression levels in TREM-1 inhibited colitic mice.
28961797	2	51	theme	endoplasmic	230:240	arg1	reticulum					242:250	endoplasmic reticulum	230:250	endoplasmic reticulum [ER] stress	230:262	Autophagy and endoplasmic reticulum [ER] stress, which activate the unfolded protein response [UPR], are closely linked and defects in these pathways contribute to the pathogenesis of inflammatory bowel disease [IBD].
28961797	11	52	theme	protein	1570:1576	arg1	levels					1589:1594	protein expression levels	1570:1594	protein expression levels	1570:1594	We observed an increase in macroautophagy [ATG1/ULK-1, ATG13, ATG5, ATG16L1, and MAP1LC3-I/II] and in CMA [HSPA8 and HSP90AA1], whereas there was a decrease in the UPR [PERK, IRE-1α, and ATF-6α] protein expression levels in TREM-1 inhibited colitic mice.
28961797	4	53	theme	aberrant	646:653	arg1	inflammation					655:666	aberrant inflammation	646:666	aberrant inflammation	646:666	We investigated whether inhibition of TREM-1 would prevent aberrant inflammation by modulating autophagy and ER stress and preventing dysbiosis.
28961797	5	54	theme	sodium	815:820	arg1	treatment					822:830	dextran sulphate sodium treatment	798:830	dextran sulphate sodium treatment	798:830	METHODS An experimental mouse model of colitis was established by dextran sulphate sodium treatment.
28961797	13	55	theme	autophagy	1762:1770	arg1	activity					1772:1779	autophagy activity	1762:1779	autophagy activity	1762:1779	CONCLUSIONS TREM-1 may represent a novel drug target for the treatment of IBD, by modulating autophagy activity and ER stress.
28961797	0	56	from	Colitis	67:73	arg1	Mice					78:81	Mice	78:81	Mice	78:81	TREM-1 Inhibition Restores Impaired Autophagy Activity and Reduces Colitis in Mice.
28961797	10	57	dep	RESULTS	1246:1252	arg1	We					1254:1255	We	1254:1255	We	1254:1255	RESULTS We confirmed that inhibition of TREM-1 attenuates the severity of colitis clinically, endoscopically and histologically.
28961797	9	58	theme	intestinal	1154:1163	arg1	microbiota					1165:1174	the intestinal microbiota	1150:1174	the intestinal microbiota	1150:1174	The composition of the intestinal microbiota was assessed by MiSeq sequencing in both LR12-treated and KO animals.
28961797	11	59	from	IRE-1α	1550:1555	arg1	[PERK					1543:1547	the UPR [PERK	1535:1547	the UPR [PERK	1535:1547	We observed an increase in macroautophagy [ATG1/ULK-1, ATG13, ATG5, ATG16L1, and MAP1LC3-I/II] and in CMA [HSPA8 and HSP90AA1], whereas there was a decrease in the UPR [PERK, IRE-1α, and ATF-6α] protein expression levels in TREM-1 inhibited colitic mice.
28961797	11	59	from	IRE-1α	1550:1555	arg1	mice					1624:1627	TREM-1 inhibited colitic mice	1599:1627	TREM-1 inhibited colitic mice	1599:1627	We observed an increase in macroautophagy [ATG1/ULK-1, ATG13, ATG5, ATG16L1, and MAP1LC3-I/II] and in CMA [HSPA8 and HSP90AA1], whereas there was a decrease in the UPR [PERK, IRE-1α, and ATF-6α] protein expression levels in TREM-1 inhibited colitic mice.
28961797	8	60	theme	macroautophagy	1026:1039	arg1	Levels					1016:1021	Levels	1016:1021	Levels of macroautophagy, chaperone-mediated autophagy [CMA], and UPR proteins	1016:1093	Levels of macroautophagy, chaperone-mediated autophagy [CMA], and UPR proteins were evaluated by western blotting.
28961797	7	61	theme	faecal	962:967	arg1	pellets					969:975	faecal pellets	962:975	faecal pellets	962:975	Colon tissues and faecal pellets of control and colitic mice were used.
28961797	4	62	theme	ER	696:697	arg1	stress					699:704	ER stress	696:704	ER stress	696:704	We investigated whether inhibition of TREM-1 would prevent aberrant inflammation by modulating autophagy and ER stress and preventing dysbiosis.
28961797	7	63	theme	Colon	944:948	arg1	tissues					950:956	Colon tissues	944:956	Colon tissues	944:956	Colon tissues and faecal pellets of control and colitic mice were used.
28961797	9	64	theme	microbiota	1165:1174	arg1	composition					1135:1145	The composition	1131:1145	The composition of the intestinal microbiota	1131:1174	The composition of the intestinal microbiota was assessed by MiSeq sequencing in both LR12-treated and KO animals.
28961797	3	65	theme	intracellular	530:542	arg1	pathogens					544:552	intracellular pathogens	530:552	intracellular pathogens	530:552	Both autophagy and UPR are deeply involved in host-microbiota interactions for the clearance of intracellular pathogens, thus contributing to dysbiosis.
28961797	11	66	theme	TREM-1	1599:1604	arg1	mice					1624:1627	TREM-1 inhibited colitic mice	1599:1627	TREM-1 inhibited colitic mice	1599:1627	We observed an increase in macroautophagy [ATG1/ULK-1, ATG13, ATG5, ATG16L1, and MAP1LC3-I/II] and in CMA [HSPA8 and HSP90AA1], whereas there was a decrease in the UPR [PERK, IRE-1α, and ATF-6α] protein expression levels in TREM-1 inhibited colitic mice.
28961797	1	67	theme	myeloid	137:143	arg1	cells-1					145:151	myeloid cells-1	137:151	myeloid cells-1	137:151	BACKGROUND AND AIMS Triggering receptor expressed on myeloid cells-1 [TREM-1] is known to amplify inflammation in several diseases.
28961797	9	68	theme	LR12-treated	1217:1228	arg1	animals					1237:1243	both LR12-treated and KO animals	1212:1243	both LR12-treated and KO animals	1212:1243	The composition of the intestinal microbiota was assessed by MiSeq sequencing in both LR12-treated and KO animals.
28961797	5	69	theme	colitis	771:777	arg1	model					762:766	An experimental mouse model	740:766	METHODS An experimental mouse model of colitis	732:777	METHODS An experimental mouse model of colitis was established by dextran sulphate sodium treatment.
28961797	7	70	used	used	1010:1013	arg2	tissues					950:956	Colon tissues	944:956	Colon tissues	944:956	Colon tissues and faecal pellets of control and colitic mice were used.
28961797	7	70	used	used	1010:1013	arg2	pellets					969:975	faecal pellets	962:975	faecal pellets	962:975	Colon tissues and faecal pellets of control and colitic mice were used.
28961797	1	71	from	inflammation	182:193	arg1	diseases					206:213	several diseases	198:213	several diseases	198:213	BACKGROUND AND AIMS Triggering receptor expressed on myeloid cells-1 [TREM-1] is known to amplify inflammation in several diseases.
28961797	4	72	theme	TREM-1	625:630	arg1	inhibition					611:620	inhibition	611:620	inhibition of TREM-1	611:630	We investigated whether inhibition of TREM-1 would prevent aberrant inflammation by modulating autophagy and ER stress and preventing dysbiosis.
28961797	11	73	dep	observed	1378:1385	arg1	whereas					1503:1509	whereas	1503:1509	whereas	1503:1509	We observed an increase in macroautophagy [ATG1/ULK-1, ATG13, ATG5, ATG16L1, and MAP1LC3-I/II] and in CMA [HSPA8 and HSP90AA1], whereas there was a decrease in the UPR [PERK, IRE-1α, and ATF-6α] protein expression levels in TREM-1 inhibited colitic mice.
28961797	11	74	theme	macroautophagy	1402:1415	arg1	[ATG1/ULK-1					1417:1427	macroautophagy [ATG1/ULK-1	1402:1427	macroautophagy [ATG1/ULK-1	1402:1427	We observed an increase in macroautophagy [ATG1/ULK-1, ATG13, ATG5, ATG16L1, and MAP1LC3-I/II] and in CMA [HSPA8 and HSP90AA1], whereas there was a decrease in the UPR [PERK, IRE-1α, and ATF-6α] protein expression levels in TREM-1 inhibited colitic mice.
28961797	2	75	theme	[ER	252:254	arg1	stress					257:262	endoplasmic reticulum [ER] stress	230:262	endoplasmic reticulum [ER] stress	230:262	Autophagy and endoplasmic reticulum [ER] stress, which activate the unfolded protein response [UPR], are closely linked and defects in these pathways contribute to the pathogenesis of inflammatory bowel disease [IBD].
28961797	7	76	theme	mice	1000:1003	arg1	tissues					950:956	Colon tissues	944:956	Colon tissues	944:956	Colon tissues and faecal pellets of control and colitic mice were used.
28961797	7	76	theme	mice	1000:1003	arg1	pellets					969:975	faecal pellets	962:975	faecal pellets	962:975	Colon tissues and faecal pellets of control and colitic mice were used.
28961797	1	77	theme	[TREM-1	153:159	arg1	BACKGROUND					84:93	BACKGROUND	84:93	BACKGROUND	84:93	BACKGROUND AND AIMS Triggering receptor expressed on myeloid cells-1 [TREM-1] is known to amplify inflammation in several diseases.
30288040	4	0	theme	apoptotic	837:845	arg1	induction					847:855	more apoptotic induction	832:855	more apoptotic induction	832:855	RESULTS Lymphoma cells treated with Dox-NCs had significantly higher intracellular Dox concentrations and more apoptotic induction, with lower P-gp expression, when compared with those treated with Dox alone.
30288040	8	1	with	consistent	1445:1454	arg1	concentration					1483:1495	low intracellular Dox concentration	1461:1495	low intracellular Dox concentration in the cardiac cell line H9C2	1461:1525	Noticeably, apoptotic myocardial cells were decreased in the Dox-NCs-treated group, when compared with the control group, which was consistent with low intracellular Dox concentration in the cardiac cell line H9C2.
30288040	9	2	theme	in	1628:1629	arg1	models					1637:1642	both in vivo and in vitro models	1611:1642	both in vivo and in vitro models	1611:1642	CONCLUSION Dox-NCs showed an anti-lymphoma effect with reduced cardiac toxicity in both in vivo and in vitro models and, therefore, could be a potential therapeutic agent in the treatment of lymphoma.
30288040	2	3	theme	Dox-loaded	280:289	arg1	NCs					320:322	NCs	320:322	NCs	320:322	The aim of this study was to explore new Dox-loaded dextran-based nano-carriers (NCs) in efficiently targeting tumor growth with less cardiac toxicity.
30288040	2	3	theme	Dox-loaded	280:289	arg1	nano-carriers					305:317	new Dox-loaded dextran-based nano-carriers	276:317	new Dox-loaded dextran-based nano-carriers (NCs)	276:323	The aim of this study was to explore new Dox-loaded dextran-based nano-carriers (NCs) in efficiently targeting tumor growth with less cardiac toxicity.
30288040	4	4	theme	Dox	809:811	arg1	concentrations					813:826	significantly higher intracellular Dox concentrations	774:826	significantly higher intracellular Dox concentrations	774:826	RESULTS Lymphoma cells treated with Dox-NCs had significantly higher intracellular Dox concentrations and more apoptotic induction, with lower P-gp expression, when compared with those treated with Dox alone.
30288040	6	5	theme	cell	1118:1121	arg1	growth					1123:1128	cell growth	1118:1128	cell growth	1118:1128	Even in the Dox-resistant cells, Dox-NCs could significantly inhibit cell growth and induce apoptosis.
30288040	3	6	theme	circulation	694:704	arg1	pH					717:718	pH 7.4	717:722	pH 7.4	717:722	METHODS Inspired by recent reports that polymeric NCs could function as sustained, controlled and targeted drug delivery systems, we developed Dox-loaded NCs which displayed a 2-fold release ratio of Dox in the mimic tumor site condition (pH 5.0 with 10 mM glutathione, GSH) as much as that in systemic circulation condition (pH 7.4).
30288040	3	6	theme	circulation	694:704	arg1	condition					706:714	systemic circulation condition	685:714	systemic circulation condition (pH 7.4)	685:723	METHODS Inspired by recent reports that polymeric NCs could function as sustained, controlled and targeted drug delivery systems, we developed Dox-loaded NCs which displayed a 2-fold release ratio of Dox in the mimic tumor site condition (pH 5.0 with 10 mM glutathione, GSH) as much as that in systemic circulation condition (pH 7.4).
30288040	0	7	theme	toxicity	140:147	arg1	reduction					119:127	synchronous reduction	107:127	synchronous reduction of cardiac toxicity	107:147	Doxorubicin-loaded dextran-based nano-carriers for highly efficient inhibition of lymphoma cell growth and synchronous reduction of cardiac toxicity.
30288040	0	7	theme	toxicity	140:147	arg1	inhibition					68:77	highly efficient inhibition	51:77	highly efficient inhibition of lymphoma cell growth	51:101	Doxorubicin-loaded dextran-based nano-carriers for highly efficient inhibition of lymphoma cell growth and synchronous reduction of cardiac toxicity.
30288040	5	8	theme	caspase-3	1027:1035	arg1	activation					1037:1046	caspase-3 activation	1027:1046	caspase-3 activation	1027:1046	The identified mechanism of action, apoptosis, was triggered through survivin reduction and caspase-3 activation.
30288040	3	9	theme	2-fold	567:572	arg1	ratio					582:586	a 2-fold release ratio	565:586	a 2-fold release ratio of Dox	565:593	METHODS Inspired by recent reports that polymeric NCs could function as sustained, controlled and targeted drug delivery systems, we developed Dox-loaded NCs which displayed a 2-fold release ratio of Dox in the mimic tumor site condition (pH 5.0 with 10 mM glutathione, GSH) as much as that in systemic circulation condition (pH 7.4).
30288040	9	10	theme	potential	1671:1679	arg1	agent					1693:1697	a potential therapeutic agent	1669:1697	a potential therapeutic agent in the treatment of lymphoma	1669:1726	CONCLUSION Dox-NCs showed an anti-lymphoma effect with reduced cardiac toxicity in both in vivo and in vitro models and, therefore, could be a potential therapeutic agent in the treatment of lymphoma.
30288040	9	11	dep	in	1628:1629	arg1	vitro					1631:1635	vitro	1631:1635	vitro	1631:1635	CONCLUSION Dox-NCs showed an anti-lymphoma effect with reduced cardiac toxicity in both in vivo and in vitro models and, therefore, could be a potential therapeutic agent in the treatment of lymphoma.
30288040	3	12	dep	condition	619:627	arg1	GSH					661:663	GSH	661:663	GSH	661:663	METHODS Inspired by recent reports that polymeric NCs could function as sustained, controlled and targeted drug delivery systems, we developed Dox-loaded NCs which displayed a 2-fold release ratio of Dox in the mimic tumor site condition (pH 5.0 with 10 mM glutathione, GSH) as much as that in systemic circulation condition (pH 7.4).
30288040	3	12	dep	condition	619:627	arg1	pH					630:631	pH 5.0	630:635	pH 5.0 with 10 mM glutathione	630:658	METHODS Inspired by recent reports that polymeric NCs could function as sustained, controlled and targeted drug delivery systems, we developed Dox-loaded NCs which displayed a 2-fold release ratio of Dox in the mimic tumor site condition (pH 5.0 with 10 mM glutathione, GSH) as much as that in systemic circulation condition (pH 7.4).
30288040	4	13	theme	lower	863:867	arg1	expression					874:883	lower P-gp expression	863:883	lower P-gp expression	863:883	RESULTS Lymphoma cells treated with Dox-NCs had significantly higher intracellular Dox concentrations and more apoptotic induction, with lower P-gp expression, when compared with those treated with Dox alone.
30288040	3	14	theme	Dox	591:593	arg1	ratio					582:586	a 2-fold release ratio	565:586	a 2-fold release ratio of Dox	565:593	METHODS Inspired by recent reports that polymeric NCs could function as sustained, controlled and targeted drug delivery systems, we developed Dox-loaded NCs which displayed a 2-fold release ratio of Dox in the mimic tumor site condition (pH 5.0 with 10 mM glutathione, GSH) as much as that in systemic circulation condition (pH 7.4).
30288040	1	15	theme	Cardiac	158:164	arg1	effects					171:177	PURPOSE Cardiac side effects	150:177	PURPOSE Cardiac side effects of doxorubicin (Dox)	150:198	PURPOSE Cardiac side effects of doxorubicin (Dox) have limited its clinical application.
30288040	8	16	theme	low	1461:1463	arg1	concentration					1483:1495	low intracellular Dox concentration	1461:1495	low intracellular Dox concentration in the cardiac cell line H9C2	1461:1525	Noticeably, apoptotic myocardial cells were decreased in the Dox-NCs-treated group, when compared with the control group, which was consistent with low intracellular Dox concentration in the cardiac cell line H9C2.
30288040	0	17	theme	lymphoma	82:89	arg1	growth					96:101	lymphoma cell growth	82:101	lymphoma cell growth	82:101	Doxorubicin-loaded dextran-based nano-carriers for highly efficient inhibition of lymphoma cell growth and synchronous reduction of cardiac toxicity.
30288040	3	18	theme	tumor	608:612	arg1	condition					619:627	the mimic tumor site condition	598:627	the mimic tumor site condition (pH 5.0 with 10 mM glutathione, GSH) as much as that in systemic circulation condition (pH 7.4)	598:723	METHODS Inspired by recent reports that polymeric NCs could function as sustained, controlled and targeted drug delivery systems, we developed Dox-loaded NCs which displayed a 2-fold release ratio of Dox in the mimic tumor site condition (pH 5.0 with 10 mM glutathione, GSH) as much as that in systemic circulation condition (pH 7.4).
30288040	8	19	theme	Dox	1479:1481	arg1	concentration					1483:1495	low intracellular Dox concentration	1461:1495	low intracellular Dox concentration in the cardiac cell line H9C2	1461:1525	Noticeably, apoptotic myocardial cells were decreased in the Dox-NCs-treated group, when compared with the control group, which was consistent with low intracellular Dox concentration in the cardiac cell line H9C2.
30288040	0	20	theme	growth	96:101	arg1	reduction					119:127	synchronous reduction	107:127	synchronous reduction of cardiac toxicity	107:147	Doxorubicin-loaded dextran-based nano-carriers for highly efficient inhibition of lymphoma cell growth and synchronous reduction of cardiac toxicity.
30288040	0	20	theme	growth	96:101	arg1	inhibition					68:77	highly efficient inhibition	51:77	highly efficient inhibition of lymphoma cell growth	51:101	Doxorubicin-loaded dextran-based nano-carriers for highly efficient inhibition of lymphoma cell growth and synchronous reduction of cardiac toxicity.
30288040	1	21	theme	doxorubicin	182:192	arg1	effects					171:177	PURPOSE Cardiac side effects	150:177	PURPOSE Cardiac side effects of doxorubicin (Dox)	150:198	PURPOSE Cardiac side effects of doxorubicin (Dox) have limited its clinical application.
30288040	8	22	theme	apoptotic	1325:1333	arg1	cells					1346:1350	apoptotic myocardial cells	1325:1350	apoptotic myocardial cells	1325:1350	Noticeably, apoptotic myocardial cells were decreased in the Dox-NCs-treated group, when compared with the control group, which was consistent with low intracellular Dox concentration in the cardiac cell line H9C2.
30288040	0	23	theme	synchronous	107:117	arg1	reduction					119:127	synchronous reduction	107:127	synchronous reduction of cardiac toxicity	107:147	Doxorubicin-loaded dextran-based nano-carriers for highly efficient inhibition of lymphoma cell growth and synchronous reduction of cardiac toxicity.
30288040	9	24	theme	CONCLUSION	1528:1537	arg1	Dox-NCs					1539:1545	CONCLUSION Dox-NCs	1528:1545	CONCLUSION Dox-NCs	1528:1545	CONCLUSION Dox-NCs showed an anti-lymphoma effect with reduced cardiac toxicity in both in vivo and in vitro models and, therefore, could be a potential therapeutic agent in the treatment of lymphoma.
30288040	8	25	theme	cardiac	1504:1510	arg1	line					1517:1520	the cardiac cell line	1500:1520	the cardiac cell line H9C2	1500:1525	Noticeably, apoptotic myocardial cells were decreased in the Dox-NCs-treated group, when compared with the control group, which was consistent with low intracellular Dox concentration in the cardiac cell line H9C2.
30288040	4	26	theme	RESULTS	726:732	arg1	cells					743:747	RESULTS Lymphoma cells	726:747	RESULTS Lymphoma cells treated with Dox-NCs	726:768	RESULTS Lymphoma cells treated with Dox-NCs had significantly higher intracellular Dox concentrations and more apoptotic induction, with lower P-gp expression, when compared with those treated with Dox alone.
30288040	7	27	theme	lymphoma	1162:1169	arg1	models					1181:1186	murine lymphoma xenograft models	1155:1186	murine lymphoma xenograft models	1155:1186	In murine lymphoma xenograft models, Dox-NCs also remarkably significantly retarded tumor growth, assessed by murine weight, and demonstrated less cytotoxicity.
30288040	5	28	theme	identified	939:948	arg1	mechanism					950:958	The identified mechanism	935:958	The identified mechanism of action, apoptosis,	935:980	The identified mechanism of action, apoptosis, was triggered through survivin reduction and caspase-3 activation.
30288040	3	29	theme	systemic	685:692	arg1	pH					717:718	pH 7.4	717:722	pH 7.4	717:722	METHODS Inspired by recent reports that polymeric NCs could function as sustained, controlled and targeted drug delivery systems, we developed Dox-loaded NCs which displayed a 2-fold release ratio of Dox in the mimic tumor site condition (pH 5.0 with 10 mM glutathione, GSH) as much as that in systemic circulation condition (pH 7.4).
30288040	3	29	theme	systemic	685:692	arg1	condition					706:714	systemic circulation condition	685:714	systemic circulation condition (pH 7.4)	685:723	METHODS Inspired by recent reports that polymeric NCs could function as sustained, controlled and targeted drug delivery systems, we developed Dox-loaded NCs which displayed a 2-fold release ratio of Dox in the mimic tumor site condition (pH 5.0 with 10 mM glutathione, GSH) as much as that in systemic circulation condition (pH 7.4).
30288040	3	30	theme	polymeric	431:439	arg1	reports					418:424	recent reports	411:424	recent reports that polymeric NCs could function as sustained, controlled and targeted drug delivery systems	411:518	METHODS Inspired by recent reports that polymeric NCs could function as sustained, controlled and targeted drug delivery systems, we developed Dox-loaded NCs which displayed a 2-fold release ratio of Dox in the mimic tumor site condition (pH 5.0 with 10 mM glutathione, GSH) as much as that in systemic circulation condition (pH 7.4).
30288040	3	30	theme	polymeric	431:439	arg1	NCs					441:443	polymeric NCs	431:443	polymeric NCs	431:443	METHODS Inspired by recent reports that polymeric NCs could function as sustained, controlled and targeted drug delivery systems, we developed Dox-loaded NCs which displayed a 2-fold release ratio of Dox in the mimic tumor site condition (pH 5.0 with 10 mM glutathione, GSH) as much as that in systemic circulation condition (pH 7.4).
30288040	3	30	theme	polymeric	431:439	arg1	systems					512:518	sustained, controlled and targeted drug delivery systems	463:518	sustained, controlled and targeted drug delivery systems	463:518	METHODS Inspired by recent reports that polymeric NCs could function as sustained, controlled and targeted drug delivery systems, we developed Dox-loaded NCs which displayed a 2-fold release ratio of Dox in the mimic tumor site condition (pH 5.0 with 10 mM glutathione, GSH) as much as that in systemic circulation condition (pH 7.4).
30288040	0	31	theme	Doxorubicin-loaded	0:17	arg1	nano-carriers					33:45	Doxorubicin-loaded dextran-based nano-carriers	0:45	Doxorubicin-loaded dextran-based nano-carriers for highly efficient inhibition of lymphoma cell growth and synchronous reduction of cardiac toxicity.	0:148	Doxorubicin-loaded dextran-based nano-carriers for highly efficient inhibition of lymphoma cell growth and synchronous reduction of cardiac toxicity.
30288040	9	32	theme	anti-lymphoma	1557:1569	arg1	effect					1571:1576	an anti-lymphoma effect	1554:1576	an anti-lymphoma effect	1554:1576	CONCLUSION Dox-NCs showed an anti-lymphoma effect with reduced cardiac toxicity in both in vivo and in vitro models and, therefore, could be a potential therapeutic agent in the treatment of lymphoma.
30288040	9	33	from	toxicity	1599:1606	arg1	models					1637:1642	both in vivo and in vitro models	1611:1642	both in vivo and in vitro models	1611:1642	CONCLUSION Dox-NCs showed an anti-lymphoma effect with reduced cardiac toxicity in both in vivo and in vitro models and, therefore, could be a potential therapeutic agent in the treatment of lymphoma.
30288040	2	34	theme	study	255:259	arg1	aim					243:245	The aim	239:245	The aim of this study	239:259	The aim of this study was to explore new Dox-loaded dextran-based nano-carriers (NCs) in efficiently targeting tumor growth with less cardiac toxicity.
30288040	3	35	theme	Dox-loaded	534:543	arg1	NCs					545:547	Dox-loaded NCs	534:547	Dox-loaded NCs which displayed a 2-fold release ratio of Dox in the mimic tumor site condition (pH 5.0 with 10 mM glutathione, GSH) as much as that in systemic circulation condition (pH 7.4)	534:723	METHODS Inspired by recent reports that polymeric NCs could function as sustained, controlled and targeted drug delivery systems, we developed Dox-loaded NCs which displayed a 2-fold release ratio of Dox in the mimic tumor site condition (pH 5.0 with 10 mM glutathione, GSH) as much as that in systemic circulation condition (pH 7.4).
30288040	9	36	theme	cardiac	1591:1597	arg1	toxicity					1599:1606	reduced cardiac toxicity	1583:1606	reduced cardiac toxicity in both in vivo and in vitro models	1583:1642	CONCLUSION Dox-NCs showed an anti-lymphoma effect with reduced cardiac toxicity in both in vivo and in vitro models and, therefore, could be a potential therapeutic agent in the treatment of lymphoma.
30288040	4	37	theme	higher	788:793	arg1	concentrations					813:826	significantly higher intracellular Dox concentrations	774:826	significantly higher intracellular Dox concentrations	774:826	RESULTS Lymphoma cells treated with Dox-NCs had significantly higher intracellular Dox concentrations and more apoptotic induction, with lower P-gp expression, when compared with those treated with Dox alone.
30288040	3	38	theme	drug	498:501	arg1	reports					418:424	recent reports	411:424	recent reports that polymeric NCs could function as sustained, controlled and targeted drug delivery systems	411:518	METHODS Inspired by recent reports that polymeric NCs could function as sustained, controlled and targeted drug delivery systems, we developed Dox-loaded NCs which displayed a 2-fold release ratio of Dox in the mimic tumor site condition (pH 5.0 with 10 mM glutathione, GSH) as much as that in systemic circulation condition (pH 7.4).
30288040	3	38	theme	drug	498:501	arg1	NCs					441:443	polymeric NCs	431:443	polymeric NCs	431:443	METHODS Inspired by recent reports that polymeric NCs could function as sustained, controlled and targeted drug delivery systems, we developed Dox-loaded NCs which displayed a 2-fold release ratio of Dox in the mimic tumor site condition (pH 5.0 with 10 mM glutathione, GSH) as much as that in systemic circulation condition (pH 7.4).
30288040	3	38	theme	drug	498:501	arg1	systems					512:518	sustained, controlled and targeted drug delivery systems	463:518	sustained, controlled and targeted drug delivery systems	463:518	METHODS Inspired by recent reports that polymeric NCs could function as sustained, controlled and targeted drug delivery systems, we developed Dox-loaded NCs which displayed a 2-fold release ratio of Dox in the mimic tumor site condition (pH 5.0 with 10 mM glutathione, GSH) as much as that in systemic circulation condition (pH 7.4).
30288040	7	39	theme	murine	1262:1267	arg1	weight					1269:1274	murine weight	1262:1274	murine weight	1262:1274	In murine lymphoma xenograft models, Dox-NCs also remarkably significantly retarded tumor growth, assessed by murine weight, and demonstrated less cytotoxicity.
30288040	9	40	theme	in	1616:1617	arg1	models					1637:1642	both in vivo and in vitro models	1611:1642	both in vivo and in vitro models	1611:1642	CONCLUSION Dox-NCs showed an anti-lymphoma effect with reduced cardiac toxicity in both in vivo and in vitro models and, therefore, could be a potential therapeutic agent in the treatment of lymphoma.
30288040	3	41	theme	mM	645:646	arg1	glutathione					648:658	10 mM glutathione	642:658	10 mM glutathione	642:658	METHODS Inspired by recent reports that polymeric NCs could function as sustained, controlled and targeted drug delivery systems, we developed Dox-loaded NCs which displayed a 2-fold release ratio of Dox in the mimic tumor site condition (pH 5.0 with 10 mM glutathione, GSH) as much as that in systemic circulation condition (pH 7.4).
30288040	8	42	theme	control	1420:1426	arg1	group					1428:1432	the control group	1416:1432	the control group	1416:1432	Noticeably, apoptotic myocardial cells were decreased in the Dox-NCs-treated group, when compared with the control group, which was consistent with low intracellular Dox concentration in the cardiac cell line H9C2.
30288040	8	42	theme	control	1420:1426	arg1	consistent					1445:1454	consistent	1445:1454	consistent	1445:1454	Noticeably, apoptotic myocardial cells were decreased in the Dox-NCs-treated group, when compared with the control group, which was consistent with low intracellular Dox concentration in the cardiac cell line H9C2.
30288040	2	43	theme	cardiac	373:379	arg1	toxicity					381:388	less cardiac toxicity	368:388	less cardiac toxicity	368:388	The aim of this study was to explore new Dox-loaded dextran-based nano-carriers (NCs) in efficiently targeting tumor growth with less cardiac toxicity.
30288040	6	44	theme	Dox-resistant	1061:1073	arg1	cells					1075:1079	the Dox-resistant cells	1057:1079	the Dox-resistant cells	1057:1079	Even in the Dox-resistant cells, Dox-NCs could significantly inhibit cell growth and induce apoptosis.
30288040	2	45	theme	dextran-based	291:303	arg1	NCs					320:322	NCs	320:322	NCs	320:322	The aim of this study was to explore new Dox-loaded dextran-based nano-carriers (NCs) in efficiently targeting tumor growth with less cardiac toxicity.
30288040	2	45	theme	dextran-based	291:303	arg1	nano-carriers					305:317	new Dox-loaded dextran-based nano-carriers	276:317	new Dox-loaded dextran-based nano-carriers (NCs)	276:323	The aim of this study was to explore new Dox-loaded dextran-based nano-carriers (NCs) in efficiently targeting tumor growth with less cardiac toxicity.
30288040	2	46	theme	tumor	350:354	arg1	growth					356:361	tumor growth	350:361	tumor growth	350:361	The aim of this study was to explore new Dox-loaded dextran-based nano-carriers (NCs) in efficiently targeting tumor growth with less cardiac toxicity.
30288040	2	47	theme	new	276:278	arg1	NCs					320:322	NCs	320:322	NCs	320:322	The aim of this study was to explore new Dox-loaded dextran-based nano-carriers (NCs) in efficiently targeting tumor growth with less cardiac toxicity.
30288040	2	47	theme	new	276:278	arg1	nano-carriers					305:317	new Dox-loaded dextran-based nano-carriers	276:317	new Dox-loaded dextran-based nano-carriers (NCs)	276:323	The aim of this study was to explore new Dox-loaded dextran-based nano-carriers (NCs) in efficiently targeting tumor growth with less cardiac toxicity.
30288040	4	48	theme	intracellular	795:807	arg1	concentrations					813:826	significantly higher intracellular Dox concentrations	774:826	significantly higher intracellular Dox concentrations	774:826	RESULTS Lymphoma cells treated with Dox-NCs had significantly higher intracellular Dox concentrations and more apoptotic induction, with lower P-gp expression, when compared with those treated with Dox alone.
30288040	3	49	theme	release	574:580	arg1	ratio					582:586	a 2-fold release ratio	565:586	a 2-fold release ratio of Dox	565:593	METHODS Inspired by recent reports that polymeric NCs could function as sustained, controlled and targeted drug delivery systems, we developed Dox-loaded NCs which displayed a 2-fold release ratio of Dox in the mimic tumor site condition (pH 5.0 with 10 mM glutathione, GSH) as much as that in systemic circulation condition (pH 7.4).
30288040	3	50	with	pH	630:631	arg1	glutathione					648:658	10 mM glutathione	642:658	10 mM glutathione	642:658	METHODS Inspired by recent reports that polymeric NCs could function as sustained, controlled and targeted drug delivery systems, we developed Dox-loaded NCs which displayed a 2-fold release ratio of Dox in the mimic tumor site condition (pH 5.0 with 10 mM glutathione, GSH) as much as that in systemic circulation condition (pH 7.4).
30288040	1	51	theme	clinical	217:224	arg1	application					226:236	its clinical application	213:236	its clinical application	213:236	PURPOSE Cardiac side effects of doxorubicin (Dox) have limited its clinical application.
30288040	9	52	theme	therapeutic	1681:1691	arg1	agent					1693:1697	a potential therapeutic agent	1669:1697	a potential therapeutic agent in the treatment of lymphoma	1669:1726	CONCLUSION Dox-NCs showed an anti-lymphoma effect with reduced cardiac toxicity in both in vivo and in vitro models and, therefore, could be a potential therapeutic agent in the treatment of lymphoma.
30288040	9	53	dep	in	1616:1617	arg1	vivo					1619:1622	vivo	1619:1622	vivo	1619:1622	CONCLUSION Dox-NCs showed an anti-lymphoma effect with reduced cardiac toxicity in both in vivo and in vitro models and, therefore, could be a potential therapeutic agent in the treatment of lymphoma.
30288040	9	54	dep	showed	1547:1552	arg1	agent					1693:1697	a potential therapeutic agent	1669:1697	a potential therapeutic agent in the treatment of lymphoma	1669:1726	CONCLUSION Dox-NCs showed an anti-lymphoma effect with reduced cardiac toxicity in both in vivo and in vitro models and, therefore, could be a potential therapeutic agent in the treatment of lymphoma.
30288040	5	55	theme	survivin	1004:1011	arg1	reduction					1013:1021	survivin reduction	1004:1021	survivin reduction	1004:1021	The identified mechanism of action, apoptosis, was triggered through survivin reduction and caspase-3 activation.
30288040	3	56	theme	site	614:617	arg1	condition					619:627	the mimic tumor site condition	598:627	the mimic tumor site condition (pH 5.0 with 10 mM glutathione, GSH) as much as that in systemic circulation condition (pH 7.4)	598:723	METHODS Inspired by recent reports that polymeric NCs could function as sustained, controlled and targeted drug delivery systems, we developed Dox-loaded NCs which displayed a 2-fold release ratio of Dox in the mimic tumor site condition (pH 5.0 with 10 mM glutathione, GSH) as much as that in systemic circulation condition (pH 7.4).
30288040	1	57	theme	PURPOSE	150:156	arg1	effects					171:177	PURPOSE Cardiac side effects	150:177	PURPOSE Cardiac side effects of doxorubicin (Dox)	150:198	PURPOSE Cardiac side effects of doxorubicin (Dox) have limited its clinical application.
30288040	4	58	theme	P-gp	869:872	arg1	expression					874:883	lower P-gp expression	863:883	lower P-gp expression	863:883	RESULTS Lymphoma cells treated with Dox-NCs had significantly higher intracellular Dox concentrations and more apoptotic induction, with lower P-gp expression, when compared with those treated with Dox alone.
30288040	8	59	theme	intracellular	1465:1477	arg1	concentration					1483:1495	low intracellular Dox concentration	1461:1495	low intracellular Dox concentration in the cardiac cell line H9C2	1461:1525	Noticeably, apoptotic myocardial cells were decreased in the Dox-NCs-treated group, when compared with the control group, which was consistent with low intracellular Dox concentration in the cardiac cell line H9C2.
30288040	0	60	theme	cell	91:94	arg1	growth					96:101	lymphoma cell growth	82:101	lymphoma cell growth	82:101	Doxorubicin-loaded dextran-based nano-carriers for highly efficient inhibition of lymphoma cell growth and synchronous reduction of cardiac toxicity.
30288040	3	61	theme	mimic	602:606	arg1	condition					619:627	the mimic tumor site condition	598:627	the mimic tumor site condition (pH 5.0 with 10 mM glutathione, GSH) as much as that in systemic circulation condition (pH 7.4)	598:723	METHODS Inspired by recent reports that polymeric NCs could function as sustained, controlled and targeted drug delivery systems, we developed Dox-loaded NCs which displayed a 2-fold release ratio of Dox in the mimic tumor site condition (pH 5.0 with 10 mM glutathione, GSH) as much as that in systemic circulation condition (pH 7.4).
30288040	1	62	theme	side	166:169	arg1	effects					171:177	PURPOSE Cardiac side effects	150:177	PURPOSE Cardiac side effects of doxorubicin (Dox)	150:198	PURPOSE Cardiac side effects of doxorubicin (Dox) have limited its clinical application.
30288040	9	63	from	agent	1693:1697	arg1	treatment					1706:1714	the treatment	1702:1714	the treatment of lymphoma	1702:1726	CONCLUSION Dox-NCs showed an anti-lymphoma effect with reduced cardiac toxicity in both in vivo and in vitro models and, therefore, could be a potential therapeutic agent in the treatment of lymphoma.
30288040	9	64	theme	lymphoma	1719:1726	arg1	treatment					1706:1714	the treatment	1702:1714	the treatment of lymphoma	1702:1726	CONCLUSION Dox-NCs showed an anti-lymphoma effect with reduced cardiac toxicity in both in vivo and in vitro models and, therefore, could be a potential therapeutic agent in the treatment of lymphoma.
30288040	7	65	theme	xenograft	1171:1179	arg1	models					1181:1186	murine lymphoma xenograft models	1155:1186	murine lymphoma xenograft models	1155:1186	In murine lymphoma xenograft models, Dox-NCs also remarkably significantly retarded tumor growth, assessed by murine weight, and demonstrated less cytotoxicity.
30288040	8	66	theme	cell	1512:1515	arg1	line					1517:1520	the cardiac cell line	1500:1520	the cardiac cell line H9C2	1500:1525	Noticeably, apoptotic myocardial cells were decreased in the Dox-NCs-treated group, when compared with the control group, which was consistent with low intracellular Dox concentration in the cardiac cell line H9C2.
30288040	4	67	contain	had	770:772	arg2	induction					847:855	more apoptotic induction	832:855	more apoptotic induction	832:855	RESULTS Lymphoma cells treated with Dox-NCs had significantly higher intracellular Dox concentrations and more apoptotic induction, with lower P-gp expression, when compared with those treated with Dox alone.
30288040	4	67	contain	had	770:772	arg1	cells					743:747	RESULTS Lymphoma cells	726:747	RESULTS Lymphoma cells treated with Dox-NCs	726:768	RESULTS Lymphoma cells treated with Dox-NCs had significantly higher intracellular Dox concentrations and more apoptotic induction, with lower P-gp expression, when compared with those treated with Dox alone.
30288040	4	67	contain	had	770:772	arg2	concentrations					813:826	significantly higher intracellular Dox concentrations	774:826	significantly higher intracellular Dox concentrations	774:826	RESULTS Lymphoma cells treated with Dox-NCs had significantly higher intracellular Dox concentrations and more apoptotic induction, with lower P-gp expression, when compared with those treated with Dox alone.
30288040	0	68	theme	cardiac	132:138	arg1	toxicity					140:147	cardiac toxicity	132:147	cardiac toxicity	132:147	Doxorubicin-loaded dextran-based nano-carriers for highly efficient inhibition of lymphoma cell growth and synchronous reduction of cardiac toxicity.
30288040	8	69	theme	myocardial	1335:1344	arg1	cells					1346:1350	apoptotic myocardial cells	1325:1350	apoptotic myocardial cells	1325:1350	Noticeably, apoptotic myocardial cells were decreased in the Dox-NCs-treated group, when compared with the control group, which was consistent with low intracellular Dox concentration in the cardiac cell line H9C2.
30288040	7	70	theme	murine	1155:1160	arg1	models					1181:1186	murine lymphoma xenograft models	1155:1186	murine lymphoma xenograft models	1155:1186	In murine lymphoma xenograft models, Dox-NCs also remarkably significantly retarded tumor growth, assessed by murine weight, and demonstrated less cytotoxicity.
30288040	0	71	theme	dextran-based	19:31	arg1	nano-carriers					33:45	Doxorubicin-loaded dextran-based nano-carriers	0:45	Doxorubicin-loaded dextran-based nano-carriers for highly efficient inhibition of lymphoma cell growth and synchronous reduction of cardiac toxicity.	0:148	Doxorubicin-loaded dextran-based nano-carriers for highly efficient inhibition of lymphoma cell growth and synchronous reduction of cardiac toxicity.
30288040	8	72	from	concentration	1483:1495	arg1	line					1517:1520	the cardiac cell line	1500:1520	the cardiac cell line H9C2	1500:1525	Noticeably, apoptotic myocardial cells were decreased in the Dox-NCs-treated group, when compared with the control group, which was consistent with low intracellular Dox concentration in the cardiac cell line H9C2.
30288040	3	73	theme	controlled	474:483	arg1	reports					418:424	recent reports	411:424	recent reports that polymeric NCs could function as sustained, controlled and targeted drug delivery systems	411:518	METHODS Inspired by recent reports that polymeric NCs could function as sustained, controlled and targeted drug delivery systems, we developed Dox-loaded NCs which displayed a 2-fold release ratio of Dox in the mimic tumor site condition (pH 5.0 with 10 mM glutathione, GSH) as much as that in systemic circulation condition (pH 7.4).
30288040	3	73	theme	controlled	474:483	arg1	NCs					441:443	polymeric NCs	431:443	polymeric NCs	431:443	METHODS Inspired by recent reports that polymeric NCs could function as sustained, controlled and targeted drug delivery systems, we developed Dox-loaded NCs which displayed a 2-fold release ratio of Dox in the mimic tumor site condition (pH 5.0 with 10 mM glutathione, GSH) as much as that in systemic circulation condition (pH 7.4).
30288040	3	73	theme	controlled	474:483	arg1	systems					512:518	sustained, controlled and targeted drug delivery systems	463:518	sustained, controlled and targeted drug delivery systems	463:518	METHODS Inspired by recent reports that polymeric NCs could function as sustained, controlled and targeted drug delivery systems, we developed Dox-loaded NCs which displayed a 2-fold release ratio of Dox in the mimic tumor site condition (pH 5.0 with 10 mM glutathione, GSH) as much as that in systemic circulation condition (pH 7.4).
30288040	0	74	theme	efficient	58:66	arg1	inhibition					68:77	highly efficient inhibition	51:77	highly efficient inhibition of lymphoma cell growth	51:101	Doxorubicin-loaded dextran-based nano-carriers for highly efficient inhibition of lymphoma cell growth and synchronous reduction of cardiac toxicity.
30288040	3	75	dep	much	669:672	arg1	as					674:675	as	674:675	as	674:675	METHODS Inspired by recent reports that polymeric NCs could function as sustained, controlled and targeted drug delivery systems, we developed Dox-loaded NCs which displayed a 2-fold release ratio of Dox in the mimic tumor site condition (pH 5.0 with 10 mM glutathione, GSH) as much as that in systemic circulation condition (pH 7.4).
30288040	9	76	theme	reduced	1583:1589	arg1	toxicity					1599:1606	reduced cardiac toxicity	1583:1606	reduced cardiac toxicity in both in vivo and in vitro models	1583:1642	CONCLUSION Dox-NCs showed an anti-lymphoma effect with reduced cardiac toxicity in both in vivo and in vitro models and, therefore, could be a potential therapeutic agent in the treatment of lymphoma.
30288040	8	77	theme	Dox-NCs-treated	1374:1388	arg1	group					1390:1394	the Dox-NCs-treated group	1370:1394	the Dox-NCs-treated group	1370:1394	Noticeably, apoptotic myocardial cells were decreased in the Dox-NCs-treated group, when compared with the control group, which was consistent with low intracellular Dox concentration in the cardiac cell line H9C2.
30288040	3	78	theme	sustained	463:471	arg1	reports					418:424	recent reports	411:424	recent reports that polymeric NCs could function as sustained, controlled and targeted drug delivery systems	411:518	METHODS Inspired by recent reports that polymeric NCs could function as sustained, controlled and targeted drug delivery systems, we developed Dox-loaded NCs which displayed a 2-fold release ratio of Dox in the mimic tumor site condition (pH 5.0 with 10 mM glutathione, GSH) as much as that in systemic circulation condition (pH 7.4).
30288040	3	78	theme	sustained	463:471	arg1	NCs					441:443	polymeric NCs	431:443	polymeric NCs	431:443	METHODS Inspired by recent reports that polymeric NCs could function as sustained, controlled and targeted drug delivery systems, we developed Dox-loaded NCs which displayed a 2-fold release ratio of Dox in the mimic tumor site condition (pH 5.0 with 10 mM glutathione, GSH) as much as that in systemic circulation condition (pH 7.4).
30288040	3	78	theme	sustained	463:471	arg1	systems					512:518	sustained, controlled and targeted drug delivery systems	463:518	sustained, controlled and targeted drug delivery systems	463:518	METHODS Inspired by recent reports that polymeric NCs could function as sustained, controlled and targeted drug delivery systems, we developed Dox-loaded NCs which displayed a 2-fold release ratio of Dox in the mimic tumor site condition (pH 5.0 with 10 mM glutathione, GSH) as much as that in systemic circulation condition (pH 7.4).
30288040	3	79	theme	recent	411:416	arg1	reports					418:424	recent reports	411:424	recent reports that polymeric NCs could function as sustained, controlled and targeted drug delivery systems	411:518	METHODS Inspired by recent reports that polymeric NCs could function as sustained, controlled and targeted drug delivery systems, we developed Dox-loaded NCs which displayed a 2-fold release ratio of Dox in the mimic tumor site condition (pH 5.0 with 10 mM glutathione, GSH) as much as that in systemic circulation condition (pH 7.4).
30288040	3	79	theme	recent	411:416	arg1	NCs					441:443	polymeric NCs	431:443	polymeric NCs	431:443	METHODS Inspired by recent reports that polymeric NCs could function as sustained, controlled and targeted drug delivery systems, we developed Dox-loaded NCs which displayed a 2-fold release ratio of Dox in the mimic tumor site condition (pH 5.0 with 10 mM glutathione, GSH) as much as that in systemic circulation condition (pH 7.4).
30288040	3	79	theme	recent	411:416	arg1	systems					512:518	sustained, controlled and targeted drug delivery systems	463:518	sustained, controlled and targeted drug delivery systems	463:518	METHODS Inspired by recent reports that polymeric NCs could function as sustained, controlled and targeted drug delivery systems, we developed Dox-loaded NCs which displayed a 2-fold release ratio of Dox in the mimic tumor site condition (pH 5.0 with 10 mM glutathione, GSH) as much as that in systemic circulation condition (pH 7.4).
30288040	3	80	theme	delivery	503:510	arg1	reports					418:424	recent reports	411:424	recent reports that polymeric NCs could function as sustained, controlled and targeted drug delivery systems	411:518	METHODS Inspired by recent reports that polymeric NCs could function as sustained, controlled and targeted drug delivery systems, we developed Dox-loaded NCs which displayed a 2-fold release ratio of Dox in the mimic tumor site condition (pH 5.0 with 10 mM glutathione, GSH) as much as that in systemic circulation condition (pH 7.4).
30288040	3	80	theme	delivery	503:510	arg1	NCs					441:443	polymeric NCs	431:443	polymeric NCs	431:443	METHODS Inspired by recent reports that polymeric NCs could function as sustained, controlled and targeted drug delivery systems, we developed Dox-loaded NCs which displayed a 2-fold release ratio of Dox in the mimic tumor site condition (pH 5.0 with 10 mM glutathione, GSH) as much as that in systemic circulation condition (pH 7.4).
30288040	3	80	theme	delivery	503:510	arg1	systems					512:518	sustained, controlled and targeted drug delivery systems	463:518	sustained, controlled and targeted drug delivery systems	463:518	METHODS Inspired by recent reports that polymeric NCs could function as sustained, controlled and targeted drug delivery systems, we developed Dox-loaded NCs which displayed a 2-fold release ratio of Dox in the mimic tumor site condition (pH 5.0 with 10 mM glutathione, GSH) as much as that in systemic circulation condition (pH 7.4).
30288040	3	81	theme	10	642:643	arg1	mM					645:646	mM	645:646	mM	645:646	METHODS Inspired by recent reports that polymeric NCs could function as sustained, controlled and targeted drug delivery systems, we developed Dox-loaded NCs which displayed a 2-fold release ratio of Dox in the mimic tumor site condition (pH 5.0 with 10 mM glutathione, GSH) as much as that in systemic circulation condition (pH 7.4).
30288040	4	82	theme	Lymphoma	734:741	arg1	cells					743:747	RESULTS Lymphoma cells	726:747	RESULTS Lymphoma cells treated with Dox-NCs	726:768	RESULTS Lymphoma cells treated with Dox-NCs had significantly higher intracellular Dox concentrations and more apoptotic induction, with lower P-gp expression, when compared with those treated with Dox alone.
30288040	3	83	theme	targeted	489:496	arg1	reports					418:424	recent reports	411:424	recent reports that polymeric NCs could function as sustained, controlled and targeted drug delivery systems	411:518	METHODS Inspired by recent reports that polymeric NCs could function as sustained, controlled and targeted drug delivery systems, we developed Dox-loaded NCs which displayed a 2-fold release ratio of Dox in the mimic tumor site condition (pH 5.0 with 10 mM glutathione, GSH) as much as that in systemic circulation condition (pH 7.4).
30288040	3	83	theme	targeted	489:496	arg1	NCs					441:443	polymeric NCs	431:443	polymeric NCs	431:443	METHODS Inspired by recent reports that polymeric NCs could function as sustained, controlled and targeted drug delivery systems, we developed Dox-loaded NCs which displayed a 2-fold release ratio of Dox in the mimic tumor site condition (pH 5.0 with 10 mM glutathione, GSH) as much as that in systemic circulation condition (pH 7.4).
30288040	3	83	theme	targeted	489:496	arg1	systems					512:518	sustained, controlled and targeted drug delivery systems	463:518	sustained, controlled and targeted drug delivery systems	463:518	METHODS Inspired by recent reports that polymeric NCs could function as sustained, controlled and targeted drug delivery systems, we developed Dox-loaded NCs which displayed a 2-fold release ratio of Dox in the mimic tumor site condition (pH 5.0 with 10 mM glutathione, GSH) as much as that in systemic circulation condition (pH 7.4).
30288040	8	84	dep	line	1517:1520	arg1	H9C2					1522:1525	H9C2	1522:1525	the cardiac cell line H9C2	1500:1525	Noticeably, apoptotic myocardial cells were decreased in the Dox-NCs-treated group, when compared with the control group, which was consistent with low intracellular Dox concentration in the cardiac cell line H9C2.
30288040	5	85	theme	action	963:968	arg1	mechanism					950:958	The identified mechanism	935:958	The identified mechanism of action, apoptosis,	935:980	The identified mechanism of action, apoptosis, was triggered through survivin reduction and caspase-3 activation.
30288040	7	86	theme	tumor	1236:1240	arg1	growth					1242:1247	tumor growth	1236:1247	tumor growth	1236:1247	In murine lymphoma xenograft models, Dox-NCs also remarkably significantly retarded tumor growth, assessed by murine weight, and demonstrated less cytotoxicity.
31125304	8	0	theme	type	995:998	arg1	strains					1000:1006	lichenicola type strains	983:1006	lichenicola type strains	983:1006	The 16S rRNA gene sequence analysis placed strain GM2012T within the family Isosphaeraceae of the order Planctomycetales with 88-89 % sequence identity to Isosphaera pallida, Aquisphaeragiovannonii, Singulisphaera acidiphila, Paludisphaera borealis and Tundrisphaera lichenicola type strains.
31125304	9	1	theme	species	1116:1122	arg1	sp					1155:1156	sp	1155:1156	sp	1155:1156	Based on the genotypic and phenotypic distinctive features of the new strain, we propose a novel genus and species Tautonia sociabilis gen. nov., sp.
31125304	9	1	theme	species	1116:1122	arg1	nov.					1149:1152	species Tautonia sociabilis gen. nov.	1116:1152	species Tautonia sociabilis gen. nov.	1116:1152	Based on the genotypic and phenotypic distinctive features of the new strain, we propose a novel genus and species Tautonia sociabilis gen. nov., sp.
31125304	2	2	theme	m	302:302	arg1	mine					309:312	a 4000 m deep mine	295:312	a 4000 m deep mine in South Africa	295:328	A novel aerobic bacterium, designated as strain GM2012T, was isolated from a microbial mat proliferating under the flow of thermal water dissipating from the wall of a 4000 m deep mine in South Africa.
31125304	8	3	theme	strain	759:764	arg1	GM2012T					766:772	strain GM2012T	759:772	strain GM2012T	759:772	The 16S rRNA gene sequence analysis placed strain GM2012T within the family Isosphaeraceae of the order Planctomycetales with 88-89 % sequence identity to Isosphaera pallida, Aquisphaeragiovannonii, Singulisphaera acidiphila, Paludisphaera borealis and Tundrisphaera lichenicola type strains.
31125304	2	4	attach	isolated	190:197	arg1	mat					216:218	a microbial mat	204:218	a microbial mat proliferating under the flow of thermal water dissipating from the wall of a 4000 m deep mine in South Africa	204:328	A novel aerobic bacterium, designated as strain GM2012T, was isolated from a microbial mat proliferating under the flow of thermal water dissipating from the wall of a 4000 m deep mine in South Africa.
31125304	2	4	attach	isolated	190:197	arg2	bacterium					145:153	A novel aerobic bacterium	129:153	A novel aerobic bacterium	129:153	A novel aerobic bacterium, designated as strain GM2012T, was isolated from a microbial mat proliferating under the flow of thermal water dissipating from the wall of a 4000 m deep mine in South Africa.
31125304	8	5	theme	lichenicola	983:993	arg1	strains					1000:1006	lichenicola type strains	983:1006	lichenicola type strains	983:1006	The 16S rRNA gene sequence analysis placed strain GM2012T within the family Isosphaeraceae of the order Planctomycetales with 88-89 % sequence identity to Isosphaera pallida, Aquisphaeragiovannonii, Singulisphaera acidiphila, Paludisphaera borealis and Tundrisphaera lichenicola type strains.
31125304	2	6	from	wall	287:290	arg1	Africa					323:328	Africa	323:328	Africa	323:328	A novel aerobic bacterium, designated as strain GM2012T, was isolated from a microbial mat proliferating under the flow of thermal water dissipating from the wall of a 4000 m deep mine in South Africa.
31125304	2	7	theme	4000	297:300	arg1	m					302:302	m	302:302	m	302:302	A novel aerobic bacterium, designated as strain GM2012T, was isolated from a microbial mat proliferating under the flow of thermal water dissipating from the wall of a 4000 m deep mine in South Africa.
31125304	8	8	with	Isosphaeraceae	792:805	arg1	identity					859:866	88-89 % sequence identity	842:866	88-89 % sequence identity to Isosphaera pallida, Aquisphaeragiovannonii, Singulisphaera acidiphila, Paludisphaera borealis and Tundrisphaera lichenicola type strains	842:1006	The 16S rRNA gene sequence analysis placed strain GM2012T within the family Isosphaeraceae of the order Planctomycetales with 88-89 % sequence identity to Isosphaera pallida, Aquisphaeragiovannonii, Singulisphaera acidiphila, Paludisphaera borealis and Tundrisphaera lichenicola type strains.
31125304	9	9	theme	sociabilis	1133:1142	arg1	sp					1155:1156	sp	1155:1156	sp	1155:1156	Based on the genotypic and phenotypic distinctive features of the new strain, we propose a novel genus and species Tautonia sociabilis gen. nov., sp.
31125304	9	9	theme	sociabilis	1133:1142	arg1	nov.					1149:1152	species Tautonia sociabilis gen. nov.	1116:1152	species Tautonia sociabilis gen. nov.	1116:1152	Based on the genotypic and phenotypic distinctive features of the new strain, we propose a novel genus and species Tautonia sociabilis gen. nov., sp.
31125304	10	10	theme	strain	1178:1183	arg1	GM2012T					1185:1191	the type strain GM2012T	1169:1191	the type strain GM2012T (=VKM B-2860,=KCTC 72013)	1169:1217	nov. with the type strain GM2012T (=VKM B-2860,=KCTC 72013).
31125304	9	11	theme	genotypic	1022:1030	arg1	features					1059:1066	the genotypic and phenotypic distinctive features	1018:1066	the genotypic and phenotypic distinctive features of the new strain	1018:1084	Based on the genotypic and phenotypic distinctive features of the new strain, we propose a novel genus and species Tautonia sociabilis gen. nov., sp.
31125304	8	12	theme	rRNA	724:727	arg1	analysis					743:750	The 16S rRNA gene sequence analysis	716:750	The 16S rRNA gene sequence analysis	716:750	The 16S rRNA gene sequence analysis placed strain GM2012T within the family Isosphaeraceae of the order Planctomycetales with 88-89 % sequence identity to Isosphaera pallida, Aquisphaeragiovannonii, Singulisphaera acidiphila, Paludisphaera borealis and Tundrisphaera lichenicola type strains.
31125304	9	13	theme	gen.	1144:1147	arg1	sp					1155:1156	sp	1155:1156	sp	1155:1156	Based on the genotypic and phenotypic distinctive features of the new strain, we propose a novel genus and species Tautonia sociabilis gen. nov., sp.
31125304	9	13	theme	gen.	1144:1147	arg1	nov.					1149:1152	species Tautonia sociabilis gen. nov.	1116:1152	species Tautonia sociabilis gen. nov.	1116:1152	Based on the genotypic and phenotypic distinctive features of the new strain, we propose a novel genus and species Tautonia sociabilis gen. nov., sp.
31125304	9	14	theme	new	1075:1077	arg1	strain					1079:1084	the new strain	1071:1084	the new strain	1071:1084	Based on the genotypic and phenotypic distinctive features of the new strain, we propose a novel genus and species Tautonia sociabilis gen. nov., sp.
31125304	8	15	theme	16S	720:722	arg1	rRNA					724:727	The 16S rRNA	716:727	The 16S rRNA gene sequence analysis	716:750	The 16S rRNA gene sequence analysis placed strain GM2012T within the family Isosphaeraceae of the order Planctomycetales with 88-89 % sequence identity to Isosphaera pallida, Aquisphaeragiovannonii, Singulisphaera acidiphila, Paludisphaera borealis and Tundrisphaera lichenicola type strains.
31125304	5	16	theme	optimal	560:566	arg1	growth					568:573	The optimal growth	556:573	The optimal growth	556:573	The optimal growth occurred at 42 °C and pH 7.5-7.7.
31125304	10	17	theme	=VKM	1194:1197	arg1	72013					1212:1216	=KCTC 72013	1206:1216	=KCTC 72013	1206:1216	nov. with the type strain GM2012T (=VKM B-2860,=KCTC 72013).
31125304	10	17	theme	=VKM	1194:1197	arg1	B-2860					1199:1204	=VKM B-2860	1194:1204	=VKM B-2860	1194:1204	nov. with the type strain GM2012T (=VKM B-2860,=KCTC 72013).
31125304	8	18	theme	family	785:790	arg1	Isosphaeraceae					792:805	the family Isosphaeraceae	781:805	the family Isosphaeraceae of the order Planctomycetales with 88-89 % sequence identity to Isosphaera pallida, Aquisphaeragiovannonii, Singulisphaera acidiphila, Paludisphaera borealis and Tundrisphaera lichenicola type strains	781:1006	The 16S rRNA gene sequence analysis placed strain GM2012T within the family Isosphaeraceae of the order Planctomycetales with 88-89 % sequence identity to Isosphaera pallida, Aquisphaeragiovannonii, Singulisphaera acidiphila, Paludisphaera borealis and Tundrisphaera lichenicola type strains.
31125304	3	19	theme	capable	364:370	arg1	cells					335:339	The cells	331:339	The cells	331:339	The cells were non-motile cocci, capable of budding, occurred in single or gathered in aggregates.
31125304	3	19	theme	capable	364:370	arg1	cocci					357:361	non-motile cocci	346:361	non-motile cocci	346:361	The cells were non-motile cocci, capable of budding, occurred in single or gathered in aggregates.
31125304	9	20	theme	distinctive	1047:1057	arg1	features					1059:1066	the genotypic and phenotypic distinctive features	1018:1066	the genotypic and phenotypic distinctive features of the new strain	1018:1084	Based on the genotypic and phenotypic distinctive features of the new strain, we propose a novel genus and species Tautonia sociabilis gen. nov., sp.
31125304	7	21	theme	DNA	697:699	arg1	G+C content					678:688	The G+C content	674:688	The G+C content of the DNA	674:699	The G+C content of the DNA was 70.1 mol%.
31125304	7	21	theme	DNA	697:699	arg1	%					713:713	70.1 mol%	705:713	70.1 mol%	705:713	The G+C content of the DNA was 70.1 mol%.
31125304	0	22	theme	sociabilis	9:18	arg1	nov.					25:28	Tautonia sociabilis gen. nov.	0:28	Tautonia sociabilis gen. nov.	0:28	Tautonia sociabilis gen. nov., sp.
31125304	8	23	dep	Paludisphaera	942:954	arg1	strains					1000:1006	lichenicola type strains	983:1006	lichenicola type strains	983:1006	The 16S rRNA gene sequence analysis placed strain GM2012T within the family Isosphaeraceae of the order Planctomycetales with 88-89 % sequence identity to Isosphaera pallida, Aquisphaeragiovannonii, Singulisphaera acidiphila, Paludisphaera borealis and Tundrisphaera lichenicola type strains.
31125304	8	23	dep	Paludisphaera	942:954	arg1	borealis					956:963	borealis	956:963	Isosphaera pallida, Aquisphaeragiovannonii, Singulisphaera acidiphila, Paludisphaera borealis	871:963	The 16S rRNA gene sequence analysis placed strain GM2012T within the family Isosphaeraceae of the order Planctomycetales with 88-89 % sequence identity to Isosphaera pallida, Aquisphaeragiovannonii, Singulisphaera acidiphila, Paludisphaera borealis and Tundrisphaera lichenicola type strains.
31125304	0	24	theme	Tautonia	0:7	arg1	nov.					25:28	Tautonia sociabilis gen. nov.	0:28	Tautonia sociabilis gen. nov.	0:28	Tautonia sociabilis gen. nov., sp.
31125304	10	25	with	nov.	1159:1162	arg1	GM2012T					1185:1191	the type strain GM2012T	1169:1191	the type strain GM2012T (=VKM B-2860,=KCTC 72013)	1169:1217	nov. with the type strain GM2012T (=VKM B-2860,=KCTC 72013).
31125304	2	26	from	mine	309:312	arg1	Africa					323:328	Africa	323:328	Africa	323:328	A novel aerobic bacterium, designated as strain GM2012T, was isolated from a microbial mat proliferating under the flow of thermal water dissipating from the wall of a 4000 m deep mine in South Africa.
31125304	6	27	theme	fatty	625:629	arg1	palmitate					642:650	palmitate	642:650	palmitate	642:650	The predominant fatty acids were palmitate, stearate and oleate.
31125304	6	27	theme	fatty	625:629	arg1	acids					631:635	The predominant fatty acids	609:635	The predominant fatty acids	609:635	The predominant fatty acids were palmitate, stearate and oleate.
31125304	2	28	theme	water	260:264	arg1	flow					244:247	the flow	240:247	the flow of thermal water dissipating from the wall of a 4000 m deep mine in South Africa	240:328	A novel aerobic bacterium, designated as strain GM2012T, was isolated from a microbial mat proliferating under the flow of thermal water dissipating from the wall of a 4000 m deep mine in South Africa.
31125304	6	29	theme	predominant	613:623	arg1	palmitate					642:650	palmitate	642:650	palmitate	642:650	The predominant fatty acids were palmitate, stearate and oleate.
31125304	6	29	theme	predominant	613:623	arg1	acids					631:635	The predominant fatty acids	609:635	The predominant fatty acids	609:635	The predominant fatty acids were palmitate, stearate and oleate.
31125304	0	30	theme	gen.	20:23	arg1	nov.					25:28	Tautonia sociabilis gen. nov.	0:28	Tautonia sociabilis gen. nov.	0:28	Tautonia sociabilis gen. nov., sp.
31125304	4	31	theme	simple	500:505	arg1	polysaccharides					518:532	polysaccharides	518:532	polysaccharides	518:532	The organism is a strictly aerobic chemoorganoheterotroph, preferring simple sugars and polysaccharides as growth substrates.
31125304	4	31	theme	simple	500:505	arg1	substrates					544:553	growth substrates	537:553	growth substrates	537:553	The organism is a strictly aerobic chemoorganoheterotroph, preferring simple sugars and polysaccharides as growth substrates.
31125304	4	31	theme	simple	500:505	arg1	sugars					507:512	simple sugars	500:512	simple sugars	500:512	The organism is a strictly aerobic chemoorganoheterotroph, preferring simple sugars and polysaccharides as growth substrates.
31125304	2	32	theme	thermal	252:258	arg1	water					260:264	thermal water	252:264	thermal water dissipating from the wall of a 4000 m deep mine in South Africa	252:328	A novel aerobic bacterium, designated as strain GM2012T, was isolated from a microbial mat proliferating under the flow of thermal water dissipating from the wall of a 4000 m deep mine in South Africa.
31125304	8	33	theme	88-89 	842:847	arg1	%					848:848	%	848:848	%	848:848	The 16S rRNA gene sequence analysis placed strain GM2012T within the family Isosphaeraceae of the order Planctomycetales with 88-89 % sequence identity to Isosphaera pallida, Aquisphaeragiovannonii, Singulisphaera acidiphila, Paludisphaera borealis and Tundrisphaera lichenicola type strains.
31125304	1	34	theme	subterranean	107:118	arg1	habitat					120:126	a 4000 m deep subterranean habitat	93:126	a 4000 m deep subterranean habitat	93:126	nov., a novel thermotolerant planctomycete, isolated from a 4000 m deep subterranean habitat.
31125304	9	35	theme	phenotypic	1036:1045	arg1	features					1059:1066	the genotypic and phenotypic distinctive features	1018:1066	the genotypic and phenotypic distinctive features of the new strain	1018:1084	Based on the genotypic and phenotypic distinctive features of the new strain, we propose a novel genus and species Tautonia sociabilis gen. nov., sp.
31125304	3	36	dep	cocci	357:361	arg1	gathered					406:413	gathered	406:413	gathered in aggregates	406:427	The cells were non-motile cocci, capable of budding, occurred in single or gathered in aggregates.
31125304	3	36	dep	cocci	357:361	arg1	occurred					384:391	occurred	384:391	occurred in single	384:401	The cells were non-motile cocci, capable of budding, occurred in single or gathered in aggregates.
31125304	9	37	theme	strain	1079:1084	arg1	features					1059:1066	the genotypic and phenotypic distinctive features	1018:1066	the genotypic and phenotypic distinctive features of the new strain	1018:1084	Based on the genotypic and phenotypic distinctive features of the new strain, we propose a novel genus and species Tautonia sociabilis gen. nov., sp.
31125304	3	38	theme	budding	375:381	arg1	capable					364:370	capable	364:370	capable	364:370	The cells were non-motile cocci, capable of budding, occurred in single or gathered in aggregates.
31125304	3	39	theme	non-motile	346:355	arg1	cells					335:339	The cells	331:339	The cells	331:339	The cells were non-motile cocci, capable of budding, occurred in single or gathered in aggregates.
31125304	3	39	theme	non-motile	346:355	arg1	cocci					357:361	non-motile cocci	346:361	non-motile cocci	346:361	The cells were non-motile cocci, capable of budding, occurred in single or gathered in aggregates.
31125304	7	40	theme	70.1 mol	705:712	arg1	G+C content					678:688	The G+C content	674:688	The G+C content of the DNA	674:699	The G+C content of the DNA was 70.1 mol%.
31125304	7	40	theme	70.1 mol	705:712	arg1	%					713:713	70.1 mol%	705:713	70.1 mol%	705:713	The G+C content of the DNA was 70.1 mol%.
31125304	2	41	theme	aerobic	137:143	arg1	bacterium					145:153	A novel aerobic bacterium	129:153	A novel aerobic bacterium	129:153	A novel aerobic bacterium, designated as strain GM2012T, was isolated from a microbial mat proliferating under the flow of thermal water dissipating from the wall of a 4000 m deep mine in South Africa.
31125304	8	42	theme	 sequence	849:857	arg1	identity					859:866	88-89 % sequence identity	842:866	88-89 % sequence identity to Isosphaera pallida, Aquisphaeragiovannonii, Singulisphaera acidiphila, Paludisphaera borealis and Tundrisphaera lichenicola type strains	842:1006	The 16S rRNA gene sequence analysis placed strain GM2012T within the family Isosphaeraceae of the order Planctomycetales with 88-89 % sequence identity to Isosphaera pallida, Aquisphaeragiovannonii, Singulisphaera acidiphila, Paludisphaera borealis and Tundrisphaera lichenicola type strains.
31125304	10	43	theme	=KCTC	1206:1210	arg1	72013					1212:1216	=KCTC 72013	1206:1216	=KCTC 72013	1206:1216	nov. with the type strain GM2012T (=VKM B-2860,=KCTC 72013).
31125304	10	43	theme	=KCTC	1206:1210	arg1	B-2860					1199:1204	=VKM B-2860	1194:1204	=VKM B-2860	1194:1204	nov. with the type strain GM2012T (=VKM B-2860,=KCTC 72013).
31125304	2	44	theme	novel	131:135	arg1	bacterium					145:153	A novel aerobic bacterium	129:153	A novel aerobic bacterium	129:153	A novel aerobic bacterium, designated as strain GM2012T, was isolated from a microbial mat proliferating under the flow of thermal water dissipating from the wall of a 4000 m deep mine in South Africa.
31125304	0	45	dep	sp	31:32	arg1	nov.					25:28	Tautonia sociabilis gen. nov.	0:28	Tautonia sociabilis gen. nov.	0:28	Tautonia sociabilis gen. nov., sp.
31125304	8	46	theme	%	848:848	arg1	identity					859:866	88-89 % sequence identity	842:866	88-89 % sequence identity to Isosphaera pallida, Aquisphaeragiovannonii, Singulisphaera acidiphila, Paludisphaera borealis and Tundrisphaera lichenicola type strains	842:1006	The 16S rRNA gene sequence analysis placed strain GM2012T within the family Isosphaeraceae of the order Planctomycetales with 88-89 % sequence identity to Isosphaera pallida, Aquisphaeragiovannonii, Singulisphaera acidiphila, Paludisphaera borealis and Tundrisphaera lichenicola type strains.
31125304	9	47	theme	Tautonia	1124:1131	arg1	sp					1155:1156	sp	1155:1156	sp	1155:1156	Based on the genotypic and phenotypic distinctive features of the new strain, we propose a novel genus and species Tautonia sociabilis gen. nov., sp.
31125304	9	47	theme	Tautonia	1124:1131	arg1	nov.					1149:1152	species Tautonia sociabilis gen. nov.	1116:1152	species Tautonia sociabilis gen. nov.	1116:1152	Based on the genotypic and phenotypic distinctive features of the new strain, we propose a novel genus and species Tautonia sociabilis gen. nov., sp.
31125304	4	48	theme	aerobic	457:463	arg1	chemoorganoheterotroph					465:486	a strictly aerobic chemoorganoheterotroph	446:486	a strictly aerobic chemoorganoheterotroph	446:486	The organism is a strictly aerobic chemoorganoheterotroph, preferring simple sugars and polysaccharides as growth substrates.
31125304	4	48	theme	aerobic	457:463	arg1	organism					434:441	The organism	430:441	The organism	430:441	The organism is a strictly aerobic chemoorganoheterotroph, preferring simple sugars and polysaccharides as growth substrates.
31125304	8	49	theme	Planctomycetales	820:835	arg1	Isosphaeraceae					792:805	the family Isosphaeraceae	781:805	the family Isosphaeraceae of the order Planctomycetales with 88-89 % sequence identity to Isosphaera pallida, Aquisphaeragiovannonii, Singulisphaera acidiphila, Paludisphaera borealis and Tundrisphaera lichenicola type strains	781:1006	The 16S rRNA gene sequence analysis placed strain GM2012T within the family Isosphaeraceae of the order Planctomycetales with 88-89 % sequence identity to Isosphaera pallida, Aquisphaeragiovannonii, Singulisphaera acidiphila, Paludisphaera borealis and Tundrisphaera lichenicola type strains.
31125304	8	50	theme	sequence	734:741	arg1	analysis					743:750	The 16S rRNA gene sequence analysis	716:750	The 16S rRNA gene sequence analysis	716:750	The 16S rRNA gene sequence analysis placed strain GM2012T within the family Isosphaeraceae of the order Planctomycetales with 88-89 % sequence identity to Isosphaera pallida, Aquisphaeragiovannonii, Singulisphaera acidiphila, Paludisphaera borealis and Tundrisphaera lichenicola type strains.
31125304	2	51	theme	strain	170:175	arg1	GM2012T					177:183	strain GM2012T	170:183	strain GM2012T	170:183	A novel aerobic bacterium, designated as strain GM2012T, was isolated from a microbial mat proliferating under the flow of thermal water dissipating from the wall of a 4000 m deep mine in South Africa.
31125304	2	52	from	Africa	323:328	arg1	wall					287:290	the wall	283:290	the wall of a 4000 m deep mine in South Africa	283:328	A novel aerobic bacterium, designated as strain GM2012T, was isolated from a microbial mat proliferating under the flow of thermal water dissipating from the wall of a 4000 m deep mine in South Africa.
31125304	1	53	theme	m	100:100	arg1	habitat					120:126	a 4000 m deep subterranean habitat	93:126	a 4000 m deep subterranean habitat	93:126	nov., a novel thermotolerant planctomycete, isolated from a 4000 m deep subterranean habitat.
31125304	8	54	theme	gene	729:732	arg1	analysis					743:750	The 16S rRNA gene sequence analysis	716:750	The 16S rRNA gene sequence analysis	716:750	The 16S rRNA gene sequence analysis placed strain GM2012T within the family Isosphaeraceae of the order Planctomycetales with 88-89 % sequence identity to Isosphaera pallida, Aquisphaeragiovannonii, Singulisphaera acidiphila, Paludisphaera borealis and Tundrisphaera lichenicola type strains.
31125304	9	55	theme	novel	1100:1104	arg1	genus					1106:1110	a novel genus	1098:1110	a novel genus	1098:1110	Based on the genotypic and phenotypic distinctive features of the new strain, we propose a novel genus and species Tautonia sociabilis gen. nov., sp.
31125304	1	56	theme	deep	102:105	arg1	habitat					120:126	a 4000 m deep subterranean habitat	93:126	a 4000 m deep subterranean habitat	93:126	nov., a novel thermotolerant planctomycete, isolated from a 4000 m deep subterranean habitat.
31125304	10	57	dep	GM2012T	1185:1191	arg1	72013					1212:1216	=KCTC 72013	1206:1216	=KCTC 72013	1206:1216	nov. with the type strain GM2012T (=VKM B-2860,=KCTC 72013).
31125304	10	57	dep	GM2012T	1185:1191	arg1	B-2860					1199:1204	=VKM B-2860	1194:1204	=VKM B-2860	1194:1204	nov. with the type strain GM2012T (=VKM B-2860,=KCTC 72013).
31125304	4	58	theme	growth	537:542	arg1	polysaccharides					518:532	polysaccharides	518:532	polysaccharides	518:532	The organism is a strictly aerobic chemoorganoheterotroph, preferring simple sugars and polysaccharides as growth substrates.
31125304	4	58	theme	growth	537:542	arg1	substrates					544:553	growth substrates	537:553	growth substrates	537:553	The organism is a strictly aerobic chemoorganoheterotroph, preferring simple sugars and polysaccharides as growth substrates.
31125304	4	58	theme	growth	537:542	arg1	sugars					507:512	simple sugars	500:512	simple sugars	500:512	The organism is a strictly aerobic chemoorganoheterotroph, preferring simple sugars and polysaccharides as growth substrates.
31125304	2	59	theme	microbial	206:214	arg1	mat					216:218	a microbial mat	204:218	a microbial mat proliferating under the flow of thermal water dissipating from the wall of a 4000 m deep mine in South Africa	204:328	A novel aerobic bacterium, designated as strain GM2012T, was isolated from a microbial mat proliferating under the flow of thermal water dissipating from the wall of a 4000 m deep mine in South Africa.
31125304	2	60	theme	mine	309:312	arg1	wall					287:290	the wall	283:290	the wall of a 4000 m deep mine in South Africa	283:328	A novel aerobic bacterium, designated as strain GM2012T, was isolated from a microbial mat proliferating under the flow of thermal water dissipating from the wall of a 4000 m deep mine in South Africa.
31125304	10	61	theme	type	1173:1176	arg1	GM2012T					1185:1191	the type strain GM2012T	1169:1191	the type strain GM2012T (=VKM B-2860,=KCTC 72013)	1169:1217	nov. with the type strain GM2012T (=VKM B-2860,=KCTC 72013).
31125304	8	62	theme	order	814:818	arg1	Planctomycetales					820:835	the order Planctomycetales	810:835	the order Planctomycetales	810:835	The 16S rRNA gene sequence analysis placed strain GM2012T within the family Isosphaeraceae of the order Planctomycetales with 88-89 % sequence identity to Isosphaera pallida, Aquisphaeragiovannonii, Singulisphaera acidiphila, Paludisphaera borealis and Tundrisphaera lichenicola type strains.
31125304	2	63	theme	deep	304:307	arg1	mine					309:312	a 4000 m deep mine	295:312	a 4000 m deep mine in South Africa	295:328	A novel aerobic bacterium, designated as strain GM2012T, was isolated from a microbial mat proliferating under the flow of thermal water dissipating from the wall of a 4000 m deep mine in South Africa.
31095981	2	0	from	characterization	414:429	arg1	composition					349:359	the phytochemical composition	331:359	the phytochemical composition of a methanol extract from leaves of E. uniflora and characterization of the isolated compounds	331:455	The aim of this study was to investigate the phytochemical composition of a methanol extract from leaves of E. uniflora and characterization of the isolated compounds.
31095981	2	0	from	characterization	414:429	arg1	extract					375:381	a methanol extract	364:381	a methanol extract from leaves of E. uniflora and characterization of the isolated compounds	364:455	The aim of this study was to investigate the phytochemical composition of a methanol extract from leaves of E. uniflora and characterization of the isolated compounds.
31095981	10	1	theme	ROS	1375:1377	arg1	levels					1365:1370	the elevated levels	1352:1370	the elevated levels of ROS and p38 phosphorylation	1352:1401	Noticeable antioxidant effects were determined in HaCaT cells: The extract reduced the elevated levels of ROS and p38 phosphorylation and increased the reduced glutathione (GSH) content induced by UVA.
31095981	16	2	theme	inflammation	2784:2795	arg1	diseases					2832:2839	diabetes, inflammation, pain and related oxidative stress diseases	2774:2839	diabetes, inflammation, pain and related oxidative stress diseases	2774:2839	CONCLUSION A plethora of substantial pharmacological properties indicates that Eugenia uniflora is a good antioxidant and a sustainable by-product with solid therapeutic potential for treating diabetes, inflammation, pain and related oxidative stress diseases.
31095981	9	3	theme	3-O-[6'-O-acetyl-	1092:1108	arg1	β-D-glucoside					1109:1121	gallic acid 3-O-[6'-O-acetyl-β-D-glucoside	1080:1121	gallic acid 3-O-[6'-O-acetyl-β-D-glucoside	1080:1121	RESULTS In this work, a new compound, gallic acid 3-O-[6'-O-acetyl-β-D-glucoside], along with 16 known plant secondary metabolites (PSM) were isolated, characterized using UV, MS, and NMR (1H, 13C, DEPT, COSY, HMQC, and HMBC).
31095981	12	4	theme	latency	1852:1858	arg1	time					1860:1863	the latency time	1848:1863	the latency time	1848:1863	In rats, central and peripheral anti-nociceptive properties were also observed: The extract reduced the number of writhing in acid induced writhing and increased the latency time in hot plate test.
31095981	16	5	theme	pain	2798:2801	arg1	diseases					2832:2839	diabetes, inflammation, pain and related oxidative stress diseases	2774:2839	diabetes, inflammation, pain and related oxidative stress diseases	2774:2839	CONCLUSION A plethora of substantial pharmacological properties indicates that Eugenia uniflora is a good antioxidant and a sustainable by-product with solid therapeutic potential for treating diabetes, inflammation, pain and related oxidative stress diseases.
31095981	3	6	theme	cell-based	538:547	arg1	model					562:566	a cell-based (HaCaT cell) model	536:566	a cell-based (HaCaT cell) model	536:566	In addition, we aimed to determine the antioxidant activities in vitro and in a cell-based (HaCaT cell) model.
31095981	15	7	theme	glucoamylase	2488:2499	arg1	α-amylase					2463:2471	human pancreatic α-amylase	2446:2471	human pancreatic α-amylase (HPA)	2446:2477	These effects might be associated with the interaction of PSM with the conserved amino acid residues of human pancreatic α-amylase (HPA), maltase glucoamylase (MGAM-C) and aldose reductase (ALR2) revealing considerable binding affinities.
31095981	15	7	theme	glucoamylase	2488:2499	arg1	glucoamylase					2488:2499	maltase glucoamylase	2480:2499	maltase glucoamylase (MGAM-C)	2480:2508	These effects might be associated with the interaction of PSM with the conserved amino acid residues of human pancreatic α-amylase (HPA), maltase glucoamylase (MGAM-C) and aldose reductase (ALR2) revealing considerable binding affinities.
31095981	15	7	theme	glucoamylase	2488:2499	arg1	reductase					2521:2529	aldose reductase	2514:2529	aldose reductase (ALR2)	2514:2536	These effects might be associated with the interaction of PSM with the conserved amino acid residues of human pancreatic α-amylase (HPA), maltase glucoamylase (MGAM-C) and aldose reductase (ALR2) revealing considerable binding affinities.
31095981	15	7	theme	glucoamylase	2488:2499	arg1	residues					2434:2441	the conserved amino acid residues	2409:2441	the conserved amino acid residues of human pancreatic α-amylase (HPA), maltase glucoamylase (MGAM-C) and aldose reductase (ALR2) revealing considerable binding affinities	2409:2578	These effects might be associated with the interaction of PSM with the conserved amino acid residues of human pancreatic α-amylase (HPA), maltase glucoamylase (MGAM-C) and aldose reductase (ALR2) revealing considerable binding affinities.
31095981	6	8	dep	MATERIALS	766:774	arg1	HMBC					836:839	HMBC	836:839	HMBC	836:839	MATERIALS AND METHODS UV, MS, and NMR (1H, 13C, DEPT, COSY, HMQC, and HMBC) were used to identify the secondary metabolites.
31095981	6	8	dep	MATERIALS	766:774	arg1	NMR					800:802	NMR	800:802	NMR	800:802	MATERIALS AND METHODS UV, MS, and NMR (1H, 13C, DEPT, COSY, HMQC, and HMBC) were used to identify the secondary metabolites.
31095981	6	8	dep	MATERIALS	766:774	arg1	UV					788:789	UV	788:789	UV	788:789	MATERIALS AND METHODS UV, MS, and NMR (1H, 13C, DEPT, COSY, HMQC, and HMBC) were used to identify the secondary metabolites.
31095981	6	8	dep	MATERIALS	766:774	arg1	1H					805:806	1H	805:806	1H	805:806	MATERIALS AND METHODS UV, MS, and NMR (1H, 13C, DEPT, COSY, HMQC, and HMBC) were used to identify the secondary metabolites.
31095981	6	8	dep	MATERIALS	766:774	arg1	COSY					820:823	COSY	820:823	COSY	820:823	MATERIALS AND METHODS UV, MS, and NMR (1H, 13C, DEPT, COSY, HMQC, and HMBC) were used to identify the secondary metabolites.
31095981	6	8	dep	MATERIALS	766:774	arg1	HMQC					826:829	HMQC	826:829	HMQC	826:829	MATERIALS AND METHODS UV, MS, and NMR (1H, 13C, DEPT, COSY, HMQC, and HMBC) were used to identify the secondary metabolites.
31095981	6	8	dep	MATERIALS	766:774	arg1	MS					792:793	MS	792:793	MS	792:793	MATERIALS AND METHODS UV, MS, and NMR (1H, 13C, DEPT, COSY, HMQC, and HMBC) were used to identify the secondary metabolites.
31095981	6	8	dep	MATERIALS	766:774	arg1	13C					809:811	13C	809:811	13C	809:811	MATERIALS AND METHODS UV, MS, and NMR (1H, 13C, DEPT, COSY, HMQC, and HMBC) were used to identify the secondary metabolites.
31095981	6	8	dep	MATERIALS	766:774	arg1	MATERIALS					766:774	MATERIALS	766:774	MATERIALS	766:774	MATERIALS AND METHODS UV, MS, and NMR (1H, 13C, DEPT, COSY, HMQC, and HMBC) were used to identify the secondary metabolites.
31095981	6	8	dep	MATERIALS	766:774	arg1	DEPT					814:817	DEPT	814:817	DEPT	814:817	MATERIALS AND METHODS UV, MS, and NMR (1H, 13C, DEPT, COSY, HMQC, and HMBC) were used to identify the secondary metabolites.
31095981	11	9	theme	substantial	1490:1500	arg1	activities					1520:1529	substantial anti-inflammatory activities	1490:1529	substantial anti-inflammatory activities	1490:1529	The extract showed substantial anti-inflammatory activities in vivo: It diminished the edema thickness in carrageenan-induced hind-paw edema rat model and lowered the leukocyte migration into the peritoneal cavity.
31095981	16	10	theme	related	2807:2813	arg1	stress					2825:2830	related oxidative stress	2807:2830	related oxidative stress	2807:2830	CONCLUSION A plethora of substantial pharmacological properties indicates that Eugenia uniflora is a good antioxidant and a sustainable by-product with solid therapeutic potential for treating diabetes, inflammation, pain and related oxidative stress diseases.
31095981	4	11	from	activities	648:657	arg1	models					675:680	relevant rat models	662:680	relevant rat models	662:680	We also studied the anti-inflammatory, analgesic, antipyretic and antidiabetic activities in relevant rat models.
31095981	14	12	theme	positive	2309:2316	arg1	glibenclamide					2327:2339	glibenclamide	2327:2339	glibenclamide	2327:2339	Moreover, the extract possessed robust anti-diabetic activity in streptozotocin (STZ) -diabetic rats: It markedly reduced the elevated serum glucose and lipid peroxidation levels and increased the insulin concentration in serum with higher potency than the positive control, glibenclamide.
31095981	14	12	theme	positive	2309:2316	arg1	control					2318:2324	the positive control	2305:2324	the positive control	2305:2324	Moreover, the extract possessed robust anti-diabetic activity in streptozotocin (STZ) -diabetic rats: It markedly reduced the elevated serum glucose and lipid peroxidation levels and increased the insulin concentration in serum with higher potency than the positive control, glibenclamide.
31095981	4	13	theme	relevant	662:669	arg1	models					675:680	relevant rat models	662:680	relevant rat models	662:680	We also studied the anti-inflammatory, analgesic, antipyretic and antidiabetic activities in relevant rat models.
31095981	16	14	theme	stress	2825:2830	arg1	diseases					2832:2839	diabetes, inflammation, pain and related oxidative stress diseases	2774:2839	diabetes, inflammation, pain and related oxidative stress diseases	2774:2839	CONCLUSION A plethora of substantial pharmacological properties indicates that Eugenia uniflora is a good antioxidant and a sustainable by-product with solid therapeutic potential for treating diabetes, inflammation, pain and related oxidative stress diseases.
31095981	14	15	theme	lipid	2205:2209	arg1	peroxidation					2211:2222	lipid peroxidation	2205:2222	lipid peroxidation	2205:2222	Moreover, the extract possessed robust anti-diabetic activity in streptozotocin (STZ) -diabetic rats: It markedly reduced the elevated serum glucose and lipid peroxidation levels and increased the insulin concentration in serum with higher potency than the positive control, glibenclamide.
31095981	1	16	theme	red	210:212	arg1	cherry					224:229	The red Brazilian cherry	206:229	The red Brazilian cherry	206:229	ETHNOPHARMACOLOGICAL RELEVANCE The red Brazilian cherry, Eugenia uniflora, is widely used in traditional medicine.
31095981	14	17	theme	insulin	2249:2255	arg1	concentration					2257:2269	the insulin concentration	2245:2269	the insulin concentration in serum with higher potency than the positive control, glibenclamide	2245:2339	Moreover, the extract possessed robust anti-diabetic activity in streptozotocin (STZ) -diabetic rats: It markedly reduced the elevated serum glucose and lipid peroxidation levels and increased the insulin concentration in serum with higher potency than the positive control, glibenclamide.
31095981	10	18	theme	glutathione	1429:1439	arg1	content					1447:1453	the reduced glutathione (GSH) content	1417:1453	the reduced glutathione (GSH) content induced by UVA	1417:1468	Noticeable antioxidant effects were determined in HaCaT cells: The extract reduced the elevated levels of ROS and p38 phosphorylation and increased the reduced glutathione (GSH) content induced by UVA.
31095981	11	19	theme	leukocyte	1638:1646	arg1	migration					1648:1656	the leukocyte migration	1634:1656	the leukocyte migration into the peritoneal cavity	1634:1683	The extract showed substantial anti-inflammatory activities in vivo: It diminished the edema thickness in carrageenan-induced hind-paw edema rat model and lowered the leukocyte migration into the peritoneal cavity.
31095981	9	20	theme	plant	1145:1149	arg1	metabolites					1161:1171	16 known plant secondary metabolites	1136:1171	16 known plant secondary metabolites (PSM)	1136:1177	RESULTS In this work, a new compound, gallic acid 3-O-[6'-O-acetyl-β-D-glucoside], along with 16 known plant secondary metabolites (PSM) were isolated, characterized using UV, MS, and NMR (1H, 13C, DEPT, COSY, HMQC, and HMBC).
31095981	9	20	theme	plant	1145:1149	arg1	PSM					1174:1176	PSM	1174:1176	PSM	1174:1176	RESULTS In this work, a new compound, gallic acid 3-O-[6'-O-acetyl-β-D-glucoside], along with 16 known plant secondary metabolites (PSM) were isolated, characterized using UV, MS, and NMR (1H, 13C, DEPT, COSY, HMQC, and HMBC).
31095981	3	21	theme	HaCaT	550:554	arg1	model					562:566	a cell-based (HaCaT cell) model	536:566	a cell-based (HaCaT cell) model	536:566	In addition, we aimed to determine the antioxidant activities in vitro and in a cell-based (HaCaT cell) model.
31095981	15	22	theme	α-amylase	2463:2471	arg1	α-amylase					2463:2471	human pancreatic α-amylase	2446:2471	human pancreatic α-amylase (HPA)	2446:2477	These effects might be associated with the interaction of PSM with the conserved amino acid residues of human pancreatic α-amylase (HPA), maltase glucoamylase (MGAM-C) and aldose reductase (ALR2) revealing considerable binding affinities.
31095981	15	22	theme	α-amylase	2463:2471	arg1	glucoamylase					2488:2499	maltase glucoamylase	2480:2499	maltase glucoamylase (MGAM-C)	2480:2508	These effects might be associated with the interaction of PSM with the conserved amino acid residues of human pancreatic α-amylase (HPA), maltase glucoamylase (MGAM-C) and aldose reductase (ALR2) revealing considerable binding affinities.
31095981	15	22	theme	α-amylase	2463:2471	arg1	reductase					2521:2529	aldose reductase	2514:2529	aldose reductase (ALR2)	2514:2536	These effects might be associated with the interaction of PSM with the conserved amino acid residues of human pancreatic α-amylase (HPA), maltase glucoamylase (MGAM-C) and aldose reductase (ALR2) revealing considerable binding affinities.
31095981	15	22	theme	α-amylase	2463:2471	arg1	residues					2434:2441	the conserved amino acid residues	2409:2441	the conserved amino acid residues of human pancreatic α-amylase (HPA), maltase glucoamylase (MGAM-C) and aldose reductase (ALR2) revealing considerable binding affinities	2409:2578	These effects might be associated with the interaction of PSM with the conserved amino acid residues of human pancreatic α-amylase (HPA), maltase glucoamylase (MGAM-C) and aldose reductase (ALR2) revealing considerable binding affinities.
31095981	12	23	theme	writhing	1800:1807	arg1	number					1790:1795	the number	1786:1795	the number of writhing	1786:1807	In rats, central and peripheral anti-nociceptive properties were also observed: The extract reduced the number of writhing in acid induced writhing and increased the latency time in hot plate test.
31095981	6	24	theme	secondary	868:876	arg1	metabolites					878:888	the secondary metabolites	864:888	the secondary metabolites	864:888	MATERIALS AND METHODS UV, MS, and NMR (1H, 13C, DEPT, COSY, HMQC, and HMBC) were used to identify the secondary metabolites.
31095981	16	25	theme	substantial	2606:2616	arg1	properties					2634:2643	substantial pharmacological properties	2606:2643	substantial pharmacological properties	2606:2643	CONCLUSION A plethora of substantial pharmacological properties indicates that Eugenia uniflora is a good antioxidant and a sustainable by-product with solid therapeutic potential for treating diabetes, inflammation, pain and related oxidative stress diseases.
31095981	5	26	theme	action	705:710	arg1	mode					697:700	The molecular mode	683:700	The molecular mode of action of the antidiabetic activities	683:741	The molecular mode of action of the antidiabetic activities was also investigated.
31095981	1	27	theme	Eugenia	232:238	arg1	uniflora					240:247	Eugenia uniflora	232:247	Eugenia uniflora	232:247	ETHNOPHARMACOLOGICAL RELEVANCE The red Brazilian cherry, Eugenia uniflora, is widely used in traditional medicine.
31095981	1	27	theme	Eugenia	232:238	arg1	RELEVANCE					196:204	ETHNOPHARMACOLOGICAL RELEVANCE	175:204	ETHNOPHARMACOLOGICAL RELEVANCE	175:204	ETHNOPHARMACOLOGICAL RELEVANCE The red Brazilian cherry, Eugenia uniflora, is widely used in traditional medicine.
31095981	12	28	theme	acid	1812:1815	arg1	writhing					1825:1832	acid induced writhing	1812:1832	acid induced writhing	1812:1832	In rats, central and peripheral anti-nociceptive properties were also observed: The extract reduced the number of writhing in acid induced writhing and increased the latency time in hot plate test.
31095981	13	29	from	temperature	1981:1991	arg1	rats					1996:1999	rats	1996:1999	rats	1996:1999	Furthermore, adequate antipyretic effects were observed: The extract reduced the elevated rectal temperature in rats after intraperitoneal injection of Brewer's yeast.
31095981	9	30	from	RESULTS	1042:1048	arg1	work					1058:1061	this work	1053:1061	this work	1053:1061	RESULTS In this work, a new compound, gallic acid 3-O-[6'-O-acetyl-β-D-glucoside], along with 16 known plant secondary metabolites (PSM) were isolated, characterized using UV, MS, and NMR (1H, 13C, DEPT, COSY, HMQC, and HMBC).
31095981	16	31	theme	properties	2634:2643	arg1	plethora					2594:2601	A plethora	2592:2601	A plethora of substantial pharmacological properties	2592:2643	CONCLUSION A plethora of substantial pharmacological properties indicates that Eugenia uniflora is a good antioxidant and a sustainable by-product with solid therapeutic potential for treating diabetes, inflammation, pain and related oxidative stress diseases.
31095981	11	32	theme	peritoneal	1667:1676	arg1	cavity					1678:1683	the peritoneal cavity	1663:1683	the peritoneal cavity	1663:1683	The extract showed substantial anti-inflammatory activities in vivo: It diminished the edema thickness in carrageenan-induced hind-paw edema rat model and lowered the leukocyte migration into the peritoneal cavity.
31095981	10	33	dep	ROS	1375:1377	arg1	phosphorylation					1387:1401	phosphorylation	1387:1401	phosphorylation	1387:1401	Noticeable antioxidant effects were determined in HaCaT cells: The extract reduced the elevated levels of ROS and p38 phosphorylation and increased the reduced glutathione (GSH) content induced by UVA.
31095981	14	34	theme	serum	2187:2191	arg1	glucose					2193:2199	the elevated serum glucose	2174:2199	the elevated serum glucose	2174:2199	Moreover, the extract possessed robust anti-diabetic activity in streptozotocin (STZ) -diabetic rats: It markedly reduced the elevated serum glucose and lipid peroxidation levels and increased the insulin concentration in serum with higher potency than the positive control, glibenclamide.
31095981	11	35	theme	edema	1606:1610	arg1	model					1616:1620	carrageenan-induced hind-paw edema rat model	1577:1620	carrageenan-induced hind-paw edema rat model	1577:1620	The extract showed substantial anti-inflammatory activities in vivo: It diminished the edema thickness in carrageenan-induced hind-paw edema rat model and lowered the leukocyte migration into the peritoneal cavity.
31095981	16	36	theme	diabetes	2774:2781	arg1	diseases					2832:2839	diabetes, inflammation, pain and related oxidative stress diseases	2774:2839	diabetes, inflammation, pain and related oxidative stress diseases	2774:2839	CONCLUSION A plethora of substantial pharmacological properties indicates that Eugenia uniflora is a good antioxidant and a sustainable by-product with solid therapeutic potential for treating diabetes, inflammation, pain and related oxidative stress diseases.
31095981	11	37	theme	carrageenan-induced	1577:1595	arg1	model					1616:1620	carrageenan-induced hind-paw edema rat model	1577:1620	carrageenan-induced hind-paw edema rat model	1577:1620	The extract showed substantial anti-inflammatory activities in vivo: It diminished the edema thickness in carrageenan-induced hind-paw edema rat model and lowered the leukocyte migration into the peritoneal cavity.
31095981	13	38	theme	intraperitoneal	2007:2021	arg1	injection					2023:2031	intraperitoneal injection	2007:2031	intraperitoneal injection of Brewer's yeast	2007:2049	Furthermore, adequate antipyretic effects were observed: The extract reduced the elevated rectal temperature in rats after intraperitoneal injection of Brewer's yeast.
31095981	16	39	with	antioxidant	2687:2697	arg1	potential					2751:2759	solid therapeutic potential	2733:2759	solid therapeutic potential	2733:2759	CONCLUSION A plethora of substantial pharmacological properties indicates that Eugenia uniflora is a good antioxidant and a sustainable by-product with solid therapeutic potential for treating diabetes, inflammation, pain and related oxidative stress diseases.
31095981	11	40	from	thickness	1564:1572	arg1	model					1616:1620	carrageenan-induced hind-paw edema rat model	1577:1620	carrageenan-induced hind-paw edema rat model	1577:1620	The extract showed substantial anti-inflammatory activities in vivo: It diminished the edema thickness in carrageenan-induced hind-paw edema rat model and lowered the leukocyte migration into the peritoneal cavity.
31095981	16	41	with	by-product	2717:2726	arg1	potential					2751:2759	solid therapeutic potential	2733:2759	solid therapeutic potential	2733:2759	CONCLUSION A plethora of substantial pharmacological properties indicates that Eugenia uniflora is a good antioxidant and a sustainable by-product with solid therapeutic potential for treating diabetes, inflammation, pain and related oxidative stress diseases.
31095981	0	42	theme	antioxidant	74:84	arg1	metabolites					32:42	secondary metabolites	22:42	secondary metabolites of Eugenia uniflora and their antioxidant, anti-inflammatory, pain killing and anti-diabetic activities	22:146	Chemical profiling of secondary metabolites of Eugenia uniflora and their antioxidant, anti-inflammatory, pain killing and anti-diabetic activities: A comprehensive approach.
31095981	15	43	theme	PSM	2400:2402	arg1	interaction					2385:2395	the interaction	2381:2395	the interaction of PSM with the conserved amino acid residues of human pancreatic α-amylase (HPA), maltase glucoamylase (MGAM-C) and aldose reductase (ALR2) revealing considerable binding affinities	2381:2578	These effects might be associated with the interaction of PSM with the conserved amino acid residues of human pancreatic α-amylase (HPA), maltase glucoamylase (MGAM-C) and aldose reductase (ALR2) revealing considerable binding affinities.
31095981	10	44	theme	antioxidant	1280:1290	arg1	effects					1292:1298	Noticeable antioxidant effects	1269:1298	Noticeable antioxidant effects	1269:1298	Noticeable antioxidant effects were determined in HaCaT cells: The extract reduced the elevated levels of ROS and p38 phosphorylation and increased the reduced glutathione (GSH) content induced by UVA.
31095981	15	45	theme	aldose	2514:2519	arg1	ALR2					2532:2535	ALR2	2532:2535	ALR2	2532:2535	These effects might be associated with the interaction of PSM with the conserved amino acid residues of human pancreatic α-amylase (HPA), maltase glucoamylase (MGAM-C) and aldose reductase (ALR2) revealing considerable binding affinities.
31095981	15	45	theme	aldose	2514:2519	arg1	reductase					2521:2529	aldose reductase	2514:2529	aldose reductase (ALR2)	2514:2536	These effects might be associated with the interaction of PSM with the conserved amino acid residues of human pancreatic α-amylase (HPA), maltase glucoamylase (MGAM-C) and aldose reductase (ALR2) revealing considerable binding affinities.
31095981	14	46	theme	-diabetic	2138:2146	arg1	rats					2148:2151	streptozotocin (STZ) -diabetic rats	2117:2151	streptozotocin (STZ) -diabetic rats	2117:2151	Moreover, the extract possessed robust anti-diabetic activity in streptozotocin (STZ) -diabetic rats: It markedly reduced the elevated serum glucose and lipid peroxidation levels and increased the insulin concentration in serum with higher potency than the positive control, glibenclamide.
31095981	5	47	theme	activities	732:741	arg1	action					705:710	action	705:710	action of the antidiabetic activities	705:741	The molecular mode of action of the antidiabetic activities was also investigated.
31095981	13	48	theme	adequate	1897:1904	arg1	effects					1918:1924	adequate antipyretic effects	1897:1924	adequate antipyretic effects	1897:1924	Furthermore, adequate antipyretic effects were observed: The extract reduced the elevated rectal temperature in rats after intraperitoneal injection of Brewer's yeast.
31095981	16	49	theme	solid	2733:2737	arg1	potential					2751:2759	solid therapeutic potential	2733:2759	solid therapeutic potential	2733:2759	CONCLUSION A plethora of substantial pharmacological properties indicates that Eugenia uniflora is a good antioxidant and a sustainable by-product with solid therapeutic potential for treating diabetes, inflammation, pain and related oxidative stress diseases.
31095981	0	50	theme	Chemical	0:7	arg1	profiling					9:17	Chemical profiling	0:17	Chemical profiling of secondary metabolites of Eugenia uniflora and their antioxidant, anti-inflammatory, pain killing and anti-diabetic activities: A comprehensive approach.	0:173	Chemical profiling of secondary metabolites of Eugenia uniflora and their antioxidant, anti-inflammatory, pain killing and anti-diabetic activities: A comprehensive approach.
31095981	2	51	theme	isolated	438:445	arg1	compounds					447:455	the isolated compounds	434:455	the isolated compounds	434:455	The aim of this study was to investigate the phytochemical composition of a methanol extract from leaves of E. uniflora and characterization of the isolated compounds.
31095981	12	52	theme	peripheral	1707:1716	arg1	properties					1735:1744	central and peripheral anti-nociceptive properties	1695:1744	central and peripheral anti-nociceptive properties	1695:1744	In rats, central and peripheral anti-nociceptive properties were also observed: The extract reduced the number of writhing in acid induced writhing and increased the latency time in hot plate test.
31095981	9	53	dep	compound	1070:1077	arg1	β-D-glucoside					1109:1121	gallic acid 3-O-[6'-O-acetyl-β-D-glucoside	1080:1121	gallic acid 3-O-[6'-O-acetyl-β-D-glucoside	1080:1121	RESULTS In this work, a new compound, gallic acid 3-O-[6'-O-acetyl-β-D-glucoside], along with 16 known plant secondary metabolites (PSM) were isolated, characterized using UV, MS, and NMR (1H, 13C, DEPT, COSY, HMQC, and HMBC).
31095981	14	54	from	activity	2105:2112	arg1	rats					2148:2151	streptozotocin (STZ) -diabetic rats	2117:2151	streptozotocin (STZ) -diabetic rats	2117:2151	Moreover, the extract possessed robust anti-diabetic activity in streptozotocin (STZ) -diabetic rats: It markedly reduced the elevated serum glucose and lipid peroxidation levels and increased the insulin concentration in serum with higher potency than the positive control, glibenclamide.
31095981	14	55	contain	possessed	2074:2082	arg2	activity					2105:2112	robust anti-diabetic activity	2084:2112	robust anti-diabetic activity in streptozotocin (STZ) -diabetic rats	2084:2151	Moreover, the extract possessed robust anti-diabetic activity in streptozotocin (STZ) -diabetic rats: It markedly reduced the elevated serum glucose and lipid peroxidation levels and increased the insulin concentration in serum with higher potency than the positive control, glibenclamide.
31095981	14	55	contain	possessed	2074:2082	arg1	extract					2066:2072	the extract	2062:2072	the extract	2062:2072	Moreover, the extract possessed robust anti-diabetic activity in streptozotocin (STZ) -diabetic rats: It markedly reduced the elevated serum glucose and lipid peroxidation levels and increased the insulin concentration in serum with higher potency than the positive control, glibenclamide.
31095981	0	56	theme	metabolites	32:42	arg1	profiling					9:17	Chemical profiling	0:17	Chemical profiling of secondary metabolites of Eugenia uniflora and their antioxidant, anti-inflammatory, pain killing and anti-diabetic activities: A comprehensive approach.	0:173	Chemical profiling of secondary metabolites of Eugenia uniflora and their antioxidant, anti-inflammatory, pain killing and anti-diabetic activities: A comprehensive approach.
31095981	8	57	theme	experimental	1020:1031	arg1	animals					1033:1039	experimental animals	1020:1039	experimental animals	1020:1039	The ani-inflammatory and antidibetic activities were studied in experimental animals.
31095981	2	58	from	leaves	388:393	arg1	composition					349:359	the phytochemical composition	331:359	the phytochemical composition of a methanol extract from leaves of E. uniflora and characterization of the isolated compounds	331:455	The aim of this study was to investigate the phytochemical composition of a methanol extract from leaves of E. uniflora and characterization of the isolated compounds.
31095981	2	58	from	leaves	388:393	arg1	extract					375:381	a methanol extract	364:381	a methanol extract from leaves of E. uniflora and characterization of the isolated compounds	364:455	The aim of this study was to investigate the phytochemical composition of a methanol extract from leaves of E. uniflora and characterization of the isolated compounds.
31095981	14	59	theme	robust	2084:2089	arg1	activity					2105:2112	robust anti-diabetic activity	2084:2112	robust anti-diabetic activity in streptozotocin (STZ) -diabetic rats	2084:2151	Moreover, the extract possessed robust anti-diabetic activity in streptozotocin (STZ) -diabetic rats: It markedly reduced the elevated serum glucose and lipid peroxidation levels and increased the insulin concentration in serum with higher potency than the positive control, glibenclamide.
31095981	0	60	theme	Eugenia	47:53	arg1	uniflora					55:62	Eugenia uniflora	47:62	Eugenia uniflora	47:62	Chemical profiling of secondary metabolites of Eugenia uniflora and their antioxidant, anti-inflammatory, pain killing and anti-diabetic activities: A comprehensive approach.
31095981	10	61	theme	elevated	1356:1363	arg1	levels					1365:1370	the elevated levels	1352:1370	the elevated levels of ROS and p38 phosphorylation	1352:1401	Noticeable antioxidant effects were determined in HaCaT cells: The extract reduced the elevated levels of ROS and p38 phosphorylation and increased the reduced glutathione (GSH) content induced by UVA.
31095981	9	62	theme	new	1066:1068	arg1	RESULTS					1042:1048	RESULTS	1042:1048	RESULTS In this work	1042:1061	RESULTS In this work, a new compound, gallic acid 3-O-[6'-O-acetyl-β-D-glucoside], along with 16 known plant secondary metabolites (PSM) were isolated, characterized using UV, MS, and NMR (1H, 13C, DEPT, COSY, HMQC, and HMBC).
31095981	9	62	theme	new	1066:1068	arg1	compound					1070:1077	a new compound	1064:1077	a new compound, gallic acid 3-O-[6'-O-acetyl-β-D-glucoside]	1064:1122	RESULTS In this work, a new compound, gallic acid 3-O-[6'-O-acetyl-β-D-glucoside], along with 16 known plant secondary metabolites (PSM) were isolated, characterized using UV, MS, and NMR (1H, 13C, DEPT, COSY, HMQC, and HMBC).
31095981	15	63	theme	pancreatic	2452:2461	arg1	HPA					2474:2476	HPA	2474:2476	HPA	2474:2476	These effects might be associated with the interaction of PSM with the conserved amino acid residues of human pancreatic α-amylase (HPA), maltase glucoamylase (MGAM-C) and aldose reductase (ALR2) revealing considerable binding affinities.
31095981	15	63	theme	pancreatic	2452:2461	arg1	α-amylase					2463:2471	human pancreatic α-amylase	2446:2471	human pancreatic α-amylase (HPA)	2446:2477	These effects might be associated with the interaction of PSM with the conserved amino acid residues of human pancreatic α-amylase (HPA), maltase glucoamylase (MGAM-C) and aldose reductase (ALR2) revealing considerable binding affinities.
31095981	12	64	theme	central	1695:1701	arg1	properties					1735:1744	central and peripheral anti-nociceptive properties	1695:1744	central and peripheral anti-nociceptive properties	1695:1744	In rats, central and peripheral anti-nociceptive properties were also observed: The extract reduced the number of writhing in acid induced writhing and increased the latency time in hot plate test.
31095981	14	65	theme	anti-diabetic	2091:2103	arg1	activity					2105:2112	robust anti-diabetic activity	2084:2112	robust anti-diabetic activity in streptozotocin (STZ) -diabetic rats	2084:2151	Moreover, the extract possessed robust anti-diabetic activity in streptozotocin (STZ) -diabetic rats: It markedly reduced the elevated serum glucose and lipid peroxidation levels and increased the insulin concentration in serum with higher potency than the positive control, glibenclamide.
31095981	11	66	theme	edema	1558:1562	arg1	thickness					1564:1572	the edema thickness	1554:1572	the edema thickness in carrageenan-induced hind-paw edema rat model	1554:1620	The extract showed substantial anti-inflammatory activities in vivo: It diminished the edema thickness in carrageenan-induced hind-paw edema rat model and lowered the leukocyte migration into the peritoneal cavity.
31095981	2	67	theme	extract	375:381	arg1	composition					349:359	the phytochemical composition	331:359	the phytochemical composition of a methanol extract from leaves of E. uniflora and characterization of the isolated compounds	331:455	The aim of this study was to investigate the phytochemical composition of a methanol extract from leaves of E. uniflora and characterization of the isolated compounds.
31095981	12	68	theme	hot	1868:1870	arg1	test					1878:1881	hot plate test	1868:1881	hot plate test	1868:1881	In rats, central and peripheral anti-nociceptive properties were also observed: The extract reduced the number of writhing in acid induced writhing and increased the latency time in hot plate test.
31095981	4	69	theme	anti-inflammatory	589:605	arg1	activities					648:657	the anti-inflammatory, analgesic, antipyretic and antidiabetic activities	585:657	the anti-inflammatory, analgesic, antipyretic and antidiabetic activities in relevant rat models	585:680	We also studied the anti-inflammatory, analgesic, antipyretic and antidiabetic activities in relevant rat models.
31095981	9	70	theme	acid	1087:1090	arg1	β-D-glucoside					1109:1121	gallic acid 3-O-[6'-O-acetyl-β-D-glucoside	1080:1121	gallic acid 3-O-[6'-O-acetyl-β-D-glucoside	1080:1121	RESULTS In this work, a new compound, gallic acid 3-O-[6'-O-acetyl-β-D-glucoside], along with 16 known plant secondary metabolites (PSM) were isolated, characterized using UV, MS, and NMR (1H, 13C, DEPT, COSY, HMQC, and HMBC).
31095981	3	71	theme	antioxidant	497:507	arg1	activities					509:518	the antioxidant activities	493:518	the antioxidant activities in vitro and in a cell-based (HaCaT cell) model	493:566	In addition, we aimed to determine the antioxidant activities in vitro and in a cell-based (HaCaT cell) model.
31095981	15	72	theme	acid	2429:2432	arg1	α-amylase					2463:2471	human pancreatic α-amylase	2446:2471	human pancreatic α-amylase (HPA)	2446:2477	These effects might be associated with the interaction of PSM with the conserved amino acid residues of human pancreatic α-amylase (HPA), maltase glucoamylase (MGAM-C) and aldose reductase (ALR2) revealing considerable binding affinities.
31095981	15	72	theme	acid	2429:2432	arg1	glucoamylase					2488:2499	maltase glucoamylase	2480:2499	maltase glucoamylase (MGAM-C)	2480:2508	These effects might be associated with the interaction of PSM with the conserved amino acid residues of human pancreatic α-amylase (HPA), maltase glucoamylase (MGAM-C) and aldose reductase (ALR2) revealing considerable binding affinities.
31095981	15	72	theme	acid	2429:2432	arg1	reductase					2521:2529	aldose reductase	2514:2529	aldose reductase (ALR2)	2514:2536	These effects might be associated with the interaction of PSM with the conserved amino acid residues of human pancreatic α-amylase (HPA), maltase glucoamylase (MGAM-C) and aldose reductase (ALR2) revealing considerable binding affinities.
31095981	15	72	theme	acid	2429:2432	arg1	residues					2434:2441	the conserved amino acid residues	2409:2441	the conserved amino acid residues of human pancreatic α-amylase (HPA), maltase glucoamylase (MGAM-C) and aldose reductase (ALR2) revealing considerable binding affinities	2409:2578	These effects might be associated with the interaction of PSM with the conserved amino acid residues of human pancreatic α-amylase (HPA), maltase glucoamylase (MGAM-C) and aldose reductase (ALR2) revealing considerable binding affinities.
31095981	9	73	dep	isolated	1184:1191	arg1	characterized					1194:1206	characterized	1194:1206	characterized using UV, MS, and NMR (1H, 13C, DEPT, COSY, HMQC, and HMBC)	1194:1266	RESULTS In this work, a new compound, gallic acid 3-O-[6'-O-acetyl-β-D-glucoside], along with 16 known plant secondary metabolites (PSM) were isolated, characterized using UV, MS, and NMR (1H, 13C, DEPT, COSY, HMQC, and HMBC).
31095981	10	74	theme	reduced	1421:1427	arg1	content					1447:1453	the reduced glutathione (GSH) content	1417:1453	the reduced glutathione (GSH) content induced by UVA	1417:1468	Noticeable antioxidant effects were determined in HaCaT cells: The extract reduced the elevated levels of ROS and p38 phosphorylation and increased the reduced glutathione (GSH) content induced by UVA.
31095981	3	75	from	activities	509:518	arg1	model					562:566	a cell-based (HaCaT cell) model	536:566	a cell-based (HaCaT cell) model	536:566	In addition, we aimed to determine the antioxidant activities in vitro and in a cell-based (HaCaT cell) model.
31095981	3	75	from	activities	509:518	arg1	vitro					523:527	vitro	523:527	vitro	523:527	In addition, we aimed to determine the antioxidant activities in vitro and in a cell-based (HaCaT cell) model.
31095981	15	76	theme	conserved	2413:2421	arg1	α-amylase					2463:2471	human pancreatic α-amylase	2446:2471	human pancreatic α-amylase (HPA)	2446:2477	These effects might be associated with the interaction of PSM with the conserved amino acid residues of human pancreatic α-amylase (HPA), maltase glucoamylase (MGAM-C) and aldose reductase (ALR2) revealing considerable binding affinities.
31095981	15	76	theme	conserved	2413:2421	arg1	glucoamylase					2488:2499	maltase glucoamylase	2480:2499	maltase glucoamylase (MGAM-C)	2480:2508	These effects might be associated with the interaction of PSM with the conserved amino acid residues of human pancreatic α-amylase (HPA), maltase glucoamylase (MGAM-C) and aldose reductase (ALR2) revealing considerable binding affinities.
31095981	15	76	theme	conserved	2413:2421	arg1	reductase					2521:2529	aldose reductase	2514:2529	aldose reductase (ALR2)	2514:2536	These effects might be associated with the interaction of PSM with the conserved amino acid residues of human pancreatic α-amylase (HPA), maltase glucoamylase (MGAM-C) and aldose reductase (ALR2) revealing considerable binding affinities.
31095981	15	76	theme	conserved	2413:2421	arg1	residues					2434:2441	the conserved amino acid residues	2409:2441	the conserved amino acid residues of human pancreatic α-amylase (HPA), maltase glucoamylase (MGAM-C) and aldose reductase (ALR2) revealing considerable binding affinities	2409:2578	These effects might be associated with the interaction of PSM with the conserved amino acid residues of human pancreatic α-amylase (HPA), maltase glucoamylase (MGAM-C) and aldose reductase (ALR2) revealing considerable binding affinities.
31095981	1	77	theme	traditional	268:278	arg1	medicine					280:287	traditional medicine	268:287	traditional medicine	268:287	ETHNOPHARMACOLOGICAL RELEVANCE The red Brazilian cherry, Eugenia uniflora, is widely used in traditional medicine.
31095981	2	78	from	composition	349:359	arg1	characterization					414:429	characterization	414:429	characterization of the isolated compounds	414:455	The aim of this study was to investigate the phytochemical composition of a methanol extract from leaves of E. uniflora and characterization of the isolated compounds.
31095981	2	78	from	composition	349:359	arg1	leaves					388:393	leaves	388:393	leaves	388:393	The aim of this study was to investigate the phytochemical composition of a methanol extract from leaves of E. uniflora and characterization of the isolated compounds.
31095981	16	79	theme	oxidative	2815:2823	arg1	stress					2825:2830	related oxidative stress	2807:2830	related oxidative stress	2807:2830	CONCLUSION A plethora of substantial pharmacological properties indicates that Eugenia uniflora is a good antioxidant and a sustainable by-product with solid therapeutic potential for treating diabetes, inflammation, pain and related oxidative stress diseases.
31095981	1	80	theme	ETHNOPHARMACOLOGICAL	175:194	arg1	uniflora					240:247	Eugenia uniflora	232:247	Eugenia uniflora	232:247	ETHNOPHARMACOLOGICAL RELEVANCE The red Brazilian cherry, Eugenia uniflora, is widely used in traditional medicine.
31095981	1	80	theme	ETHNOPHARMACOLOGICAL	175:194	arg1	RELEVANCE					196:204	ETHNOPHARMACOLOGICAL RELEVANCE	175:204	ETHNOPHARMACOLOGICAL RELEVANCE	175:204	ETHNOPHARMACOLOGICAL RELEVANCE The red Brazilian cherry, Eugenia uniflora, is widely used in traditional medicine.
31095981	15	81	theme	considerable	2548:2559	arg1	affinities					2569:2578	considerable binding affinities	2548:2578	considerable binding affinities	2548:2578	These effects might be associated with the interaction of PSM with the conserved amino acid residues of human pancreatic α-amylase (HPA), maltase glucoamylase (MGAM-C) and aldose reductase (ALR2) revealing considerable binding affinities.
31095981	16	82	theme	good	2682:2685	arg1	antioxidant					2687:2697	a good antioxidant	2680:2697	a good antioxidant	2680:2697	CONCLUSION A plethora of substantial pharmacological properties indicates that Eugenia uniflora is a good antioxidant and a sustainable by-product with solid therapeutic potential for treating diabetes, inflammation, pain and related oxidative stress diseases.
31095981	16	82	theme	good	2682:2685	arg1	uniflora					2668:2675	Eugenia uniflora	2660:2675	Eugenia uniflora	2660:2675	CONCLUSION A plethora of substantial pharmacological properties indicates that Eugenia uniflora is a good antioxidant and a sustainable by-product with solid therapeutic potential for treating diabetes, inflammation, pain and related oxidative stress diseases.
31095981	4	83	theme	antidiabetic	635:646	arg1	activities					648:657	the anti-inflammatory, analgesic, antipyretic and antidiabetic activities	585:657	the anti-inflammatory, analgesic, antipyretic and antidiabetic activities in relevant rat models	585:680	We also studied the anti-inflammatory, analgesic, antipyretic and antidiabetic activities in relevant rat models.
31095981	11	84	theme	anti-inflammatory	1502:1518	arg1	activities					1520:1529	substantial anti-inflammatory activities	1490:1529	substantial anti-inflammatory activities	1490:1529	The extract showed substantial anti-inflammatory activities in vivo: It diminished the edema thickness in carrageenan-induced hind-paw edema rat model and lowered the leukocyte migration into the peritoneal cavity.
31095981	14	85	theme	peroxidation	2211:2222	arg1	levels					2224:2229	the elevated serum glucose and lipid peroxidation levels	2174:2229	the elevated serum glucose and lipid peroxidation levels	2174:2229	Moreover, the extract possessed robust anti-diabetic activity in streptozotocin (STZ) -diabetic rats: It markedly reduced the elevated serum glucose and lipid peroxidation levels and increased the insulin concentration in serum with higher potency than the positive control, glibenclamide.
31095981	3	86	theme	cell	556:559	arg1	model					562:566	a cell-based (HaCaT cell) model	536:566	a cell-based (HaCaT cell) model	536:566	In addition, we aimed to determine the antioxidant activities in vitro and in a cell-based (HaCaT cell) model.
31095981	15	87	theme	maltase	2480:2486	arg1	MGAM-C					2502:2507	MGAM-C	2502:2507	MGAM-C	2502:2507	These effects might be associated with the interaction of PSM with the conserved amino acid residues of human pancreatic α-amylase (HPA), maltase glucoamylase (MGAM-C) and aldose reductase (ALR2) revealing considerable binding affinities.
31095981	15	87	theme	maltase	2480:2486	arg1	glucoamylase					2488:2499	maltase glucoamylase	2480:2499	maltase glucoamylase (MGAM-C)	2480:2508	These effects might be associated with the interaction of PSM with the conserved amino acid residues of human pancreatic α-amylase (HPA), maltase glucoamylase (MGAM-C) and aldose reductase (ALR2) revealing considerable binding affinities.
31095981	16	88	dep	CONCLUSION	2581:2590	arg1	indicates					2645:2653	indicates	2645:2653	indicates that Eugenia uniflora is a good antioxidant and a sustainable by-product with solid therapeutic potential for treating diabetes, inflammation, pain and related oxidative stress diseases	2645:2839	CONCLUSION A plethora of substantial pharmacological properties indicates that Eugenia uniflora is a good antioxidant and a sustainable by-product with solid therapeutic potential for treating diabetes, inflammation, pain and related oxidative stress diseases.
31095981	12	89	theme	induced	1817:1823	arg1	writhing					1825:1832	acid induced writhing	1812:1832	acid induced writhing	1812:1832	In rats, central and peripheral anti-nociceptive properties were also observed: The extract reduced the number of writhing in acid induced writhing and increased the latency time in hot plate test.
31095981	0	90	theme	anti-diabetic	123:135	arg1	activities					137:146	anti-diabetic activities	123:146	anti-diabetic activities	123:146	Chemical profiling of secondary metabolites of Eugenia uniflora and their antioxidant, anti-inflammatory, pain killing and anti-diabetic activities: A comprehensive approach.
31095981	9	91	theme	known	1139:1143	arg1	metabolites					1161:1171	16 known plant secondary metabolites	1136:1171	16 known plant secondary metabolites (PSM)	1136:1177	RESULTS In this work, a new compound, gallic acid 3-O-[6'-O-acetyl-β-D-glucoside], along with 16 known plant secondary metabolites (PSM) were isolated, characterized using UV, MS, and NMR (1H, 13C, DEPT, COSY, HMQC, and HMBC).
31095981	9	91	theme	known	1139:1143	arg1	PSM					1174:1176	PSM	1174:1176	PSM	1174:1176	RESULTS In this work, a new compound, gallic acid 3-O-[6'-O-acetyl-β-D-glucoside], along with 16 known plant secondary metabolites (PSM) were isolated, characterized using UV, MS, and NMR (1H, 13C, DEPT, COSY, HMQC, and HMBC).
31095981	2	92	theme	study	306:310	arg1	aim					294:296	The aim	290:296	The aim of this study	290:310	The aim of this study was to investigate the phytochemical composition of a methanol extract from leaves of E. uniflora and characterization of the isolated compounds.
31095981	1	93	theme	Brazilian	214:222	arg1	cherry					224:229	The red Brazilian cherry	206:229	The red Brazilian cherry	206:229	ETHNOPHARMACOLOGICAL RELEVANCE The red Brazilian cherry, Eugenia uniflora, is widely used in traditional medicine.
31095981	16	94	theme	sustainable	2705:2715	arg1	by-product					2717:2726	a sustainable by-product	2703:2726	a sustainable by-product with solid therapeutic potential for treating diabetes, inflammation, pain and related oxidative stress diseases	2703:2839	CONCLUSION A plethora of substantial pharmacological properties indicates that Eugenia uniflora is a good antioxidant and a sustainable by-product with solid therapeutic potential for treating diabetes, inflammation, pain and related oxidative stress diseases.
31095981	12	95	located	observed	1756:1763	arg1	rats					1689:1692	rats	1689:1692	rats	1689:1692	In rats, central and peripheral anti-nociceptive properties were also observed: The extract reduced the number of writhing in acid induced writhing and increased the latency time in hot plate test.
31095981	12	95	located	observed	1756:1763	arg2	properties					1735:1744	central and peripheral anti-nociceptive properties	1695:1744	central and peripheral anti-nociceptive properties	1695:1744	In rats, central and peripheral anti-nociceptive properties were also observed: The extract reduced the number of writhing in acid induced writhing and increased the latency time in hot plate test.
31095981	9	96	theme	secondary	1151:1159	arg1	metabolites					1161:1171	16 known plant secondary metabolites	1136:1171	16 known plant secondary metabolites (PSM)	1136:1177	RESULTS In this work, a new compound, gallic acid 3-O-[6'-O-acetyl-β-D-glucoside], along with 16 known plant secondary metabolites (PSM) were isolated, characterized using UV, MS, and NMR (1H, 13C, DEPT, COSY, HMQC, and HMBC).
31095981	9	96	theme	secondary	1151:1159	arg1	PSM					1174:1176	PSM	1174:1176	PSM	1174:1176	RESULTS In this work, a new compound, gallic acid 3-O-[6'-O-acetyl-β-D-glucoside], along with 16 known plant secondary metabolites (PSM) were isolated, characterized using UV, MS, and NMR (1H, 13C, DEPT, COSY, HMQC, and HMBC).
31095981	15	97	with	interaction	2385:2395	arg1	α-amylase					2463:2471	human pancreatic α-amylase	2446:2471	human pancreatic α-amylase (HPA)	2446:2477	These effects might be associated with the interaction of PSM with the conserved amino acid residues of human pancreatic α-amylase (HPA), maltase glucoamylase (MGAM-C) and aldose reductase (ALR2) revealing considerable binding affinities.
31095981	15	97	with	interaction	2385:2395	arg1	glucoamylase					2488:2499	maltase glucoamylase	2480:2499	maltase glucoamylase (MGAM-C)	2480:2508	These effects might be associated with the interaction of PSM with the conserved amino acid residues of human pancreatic α-amylase (HPA), maltase glucoamylase (MGAM-C) and aldose reductase (ALR2) revealing considerable binding affinities.
31095981	15	97	with	interaction	2385:2395	arg1	reductase					2521:2529	aldose reductase	2514:2529	aldose reductase (ALR2)	2514:2536	These effects might be associated with the interaction of PSM with the conserved amino acid residues of human pancreatic α-amylase (HPA), maltase glucoamylase (MGAM-C) and aldose reductase (ALR2) revealing considerable binding affinities.
31095981	15	97	with	interaction	2385:2395	arg1	residues					2434:2441	the conserved amino acid residues	2409:2441	the conserved amino acid residues of human pancreatic α-amylase (HPA), maltase glucoamylase (MGAM-C) and aldose reductase (ALR2) revealing considerable binding affinities	2409:2578	These effects might be associated with the interaction of PSM with the conserved amino acid residues of human pancreatic α-amylase (HPA), maltase glucoamylase (MGAM-C) and aldose reductase (ALR2) revealing considerable binding affinities.
31095981	14	98	theme	higher	2285:2290	arg1	potency					2292:2298	higher potency	2285:2298	higher potency than the positive control, glibenclamide	2285:2339	Moreover, the extract possessed robust anti-diabetic activity in streptozotocin (STZ) -diabetic rats: It markedly reduced the elevated serum glucose and lipid peroxidation levels and increased the insulin concentration in serum with higher potency than the positive control, glibenclamide.
31095981	4	99	theme	antipyretic	619:629	arg1	activities					648:657	the anti-inflammatory, analgesic, antipyretic and antidiabetic activities	585:657	the anti-inflammatory, analgesic, antipyretic and antidiabetic activities in relevant rat models	585:680	We also studied the anti-inflammatory, analgesic, antipyretic and antidiabetic activities in relevant rat models.
31095981	0	100	theme	comprehensive	151:163	arg1	approach					165:172	A comprehensive approach	149:172	Chemical profiling of secondary metabolites of Eugenia uniflora and their antioxidant, anti-inflammatory, pain killing and anti-diabetic activities: A comprehensive approach.	0:173	Chemical profiling of secondary metabolites of Eugenia uniflora and their antioxidant, anti-inflammatory, pain killing and anti-diabetic activities: A comprehensive approach.
31095981	7	101	theme	HaCaT	943:947	arg1	cells					949:953	HaCaT cells	943:953	HaCaT cells	943:953	Antioxidant effects were determined in vitro and in HaCaT cells.
31095981	5	102	theme	molecular	687:695	arg1	mode					697:700	The molecular mode	683:700	The molecular mode of action of the antidiabetic activities	683:741	The molecular mode of action of the antidiabetic activities was also investigated.
31095981	16	103	theme	pharmacological	2618:2632	arg1	properties					2634:2643	substantial pharmacological properties	2606:2643	substantial pharmacological properties	2606:2643	CONCLUSION A plethora of substantial pharmacological properties indicates that Eugenia uniflora is a good antioxidant and a sustainable by-product with solid therapeutic potential for treating diabetes, inflammation, pain and related oxidative stress diseases.
31095981	14	104	theme	glucose	2193:2199	arg1	levels					2224:2229	the elevated serum glucose and lipid peroxidation levels	2174:2229	the elevated serum glucose and lipid peroxidation levels	2174:2229	Moreover, the extract possessed robust anti-diabetic activity in streptozotocin (STZ) -diabetic rats: It markedly reduced the elevated serum glucose and lipid peroxidation levels and increased the insulin concentration in serum with higher potency than the positive control, glibenclamide.
31095981	6	105	used	used	847:850	arg2	COSY					820:823	COSY	820:823	COSY	820:823	MATERIALS AND METHODS UV, MS, and NMR (1H, 13C, DEPT, COSY, HMQC, and HMBC) were used to identify the secondary metabolites.
31095981	6	105	used	used	847:850	arg2	13C					809:811	13C	809:811	13C	809:811	MATERIALS AND METHODS UV, MS, and NMR (1H, 13C, DEPT, COSY, HMQC, and HMBC) were used to identify the secondary metabolites.
31095981	6	105	used	used	847:850	arg2	HMQC					826:829	HMQC	826:829	HMQC	826:829	MATERIALS AND METHODS UV, MS, and NMR (1H, 13C, DEPT, COSY, HMQC, and HMBC) were used to identify the secondary metabolites.
31095981	6	105	used	used	847:850	arg2	HMBC					836:839	HMBC	836:839	HMBC	836:839	MATERIALS AND METHODS UV, MS, and NMR (1H, 13C, DEPT, COSY, HMQC, and HMBC) were used to identify the secondary metabolites.
31095981	6	105	used	used	847:850	arg2	DEPT					814:817	DEPT	814:817	DEPT	814:817	MATERIALS AND METHODS UV, MS, and NMR (1H, 13C, DEPT, COSY, HMQC, and HMBC) were used to identify the secondary metabolites.
31095981	6	105	used	used	847:850	arg2	MATERIALS					766:774	MATERIALS	766:774	MATERIALS	766:774	MATERIALS AND METHODS UV, MS, and NMR (1H, 13C, DEPT, COSY, HMQC, and HMBC) were used to identify the secondary metabolites.
31095981	6	105	used	used	847:850	arg2	UV					788:789	UV	788:789	UV	788:789	MATERIALS AND METHODS UV, MS, and NMR (1H, 13C, DEPT, COSY, HMQC, and HMBC) were used to identify the secondary metabolites.
31095981	6	105	used	used	847:850	arg2	METHODS					780:786	METHODS	780:786	METHODS	780:786	MATERIALS AND METHODS UV, MS, and NMR (1H, 13C, DEPT, COSY, HMQC, and HMBC) were used to identify the secondary metabolites.
31095981	6	105	used	used	847:850	arg2	NMR					800:802	NMR	800:802	NMR	800:802	MATERIALS AND METHODS UV, MS, and NMR (1H, 13C, DEPT, COSY, HMQC, and HMBC) were used to identify the secondary metabolites.
31095981	6	105	used	used	847:850	arg2	MS					792:793	MS	792:793	MS	792:793	MATERIALS AND METHODS UV, MS, and NMR (1H, 13C, DEPT, COSY, HMQC, and HMBC) were used to identify the secondary metabolites.
31095981	6	105	used	used	847:850	arg2	1H					805:806	1H	805:806	1H	805:806	MATERIALS AND METHODS UV, MS, and NMR (1H, 13C, DEPT, COSY, HMQC, and HMBC) were used to identify the secondary metabolites.
31095981	14	106	from	concentration	2257:2269	arg1	serum					2274:2278	serum	2274:2278	serum	2274:2278	Moreover, the extract possessed robust anti-diabetic activity in streptozotocin (STZ) -diabetic rats: It markedly reduced the elevated serum glucose and lipid peroxidation levels and increased the insulin concentration in serum with higher potency than the positive control, glibenclamide.
31095981	14	107	theme	elevated	2178:2185	arg1	glucose					2193:2199	the elevated serum glucose	2174:2199	the elevated serum glucose	2174:2199	Moreover, the extract possessed robust anti-diabetic activity in streptozotocin (STZ) -diabetic rats: It markedly reduced the elevated serum glucose and lipid peroxidation levels and increased the insulin concentration in serum with higher potency than the positive control, glibenclamide.
31095981	11	108	theme	rat	1612:1614	arg1	model					1616:1620	carrageenan-induced hind-paw edema rat model	1577:1620	carrageenan-induced hind-paw edema rat model	1577:1620	The extract showed substantial anti-inflammatory activities in vivo: It diminished the edema thickness in carrageenan-induced hind-paw edema rat model and lowered the leukocyte migration into the peritoneal cavity.
31095981	16	109	theme	Eugenia	2660:2666	arg1	antioxidant					2687:2697	a good antioxidant	2680:2697	a good antioxidant	2680:2697	CONCLUSION A plethora of substantial pharmacological properties indicates that Eugenia uniflora is a good antioxidant and a sustainable by-product with solid therapeutic potential for treating diabetes, inflammation, pain and related oxidative stress diseases.
31095981	16	109	theme	Eugenia	2660:2666	arg1	uniflora					2668:2675	Eugenia uniflora	2660:2675	Eugenia uniflora	2660:2675	CONCLUSION A plethora of substantial pharmacological properties indicates that Eugenia uniflora is a good antioxidant and a sustainable by-product with solid therapeutic potential for treating diabetes, inflammation, pain and related oxidative stress diseases.
31095981	4	110	theme	rat	671:673	arg1	models					675:680	relevant rat models	662:680	relevant rat models	662:680	We also studied the anti-inflammatory, analgesic, antipyretic and antidiabetic activities in relevant rat models.
31095981	11	111	theme	hind-paw	1597:1604	arg1	model					1616:1620	carrageenan-induced hind-paw edema rat model	1577:1620	carrageenan-induced hind-paw edema rat model	1577:1620	The extract showed substantial anti-inflammatory activities in vivo: It diminished the edema thickness in carrageenan-induced hind-paw edema rat model and lowered the leukocyte migration into the peritoneal cavity.
31095981	15	112	theme	reductase	2521:2529	arg1	α-amylase					2463:2471	human pancreatic α-amylase	2446:2471	human pancreatic α-amylase (HPA)	2446:2477	These effects might be associated with the interaction of PSM with the conserved amino acid residues of human pancreatic α-amylase (HPA), maltase glucoamylase (MGAM-C) and aldose reductase (ALR2) revealing considerable binding affinities.
31095981	15	112	theme	reductase	2521:2529	arg1	glucoamylase					2488:2499	maltase glucoamylase	2480:2499	maltase glucoamylase (MGAM-C)	2480:2508	These effects might be associated with the interaction of PSM with the conserved amino acid residues of human pancreatic α-amylase (HPA), maltase glucoamylase (MGAM-C) and aldose reductase (ALR2) revealing considerable binding affinities.
31095981	15	112	theme	reductase	2521:2529	arg1	reductase					2521:2529	aldose reductase	2514:2529	aldose reductase (ALR2)	2514:2536	These effects might be associated with the interaction of PSM with the conserved amino acid residues of human pancreatic α-amylase (HPA), maltase glucoamylase (MGAM-C) and aldose reductase (ALR2) revealing considerable binding affinities.
31095981	15	112	theme	reductase	2521:2529	arg1	residues					2434:2441	the conserved amino acid residues	2409:2441	the conserved amino acid residues of human pancreatic α-amylase (HPA), maltase glucoamylase (MGAM-C) and aldose reductase (ALR2) revealing considerable binding affinities	2409:2578	These effects might be associated with the interaction of PSM with the conserved amino acid residues of human pancreatic α-amylase (HPA), maltase glucoamylase (MGAM-C) and aldose reductase (ALR2) revealing considerable binding affinities.
31095981	9	113	dep	NMR	1226:1228	arg1	HMQC					1252:1255	HMQC	1252:1255	HMQC	1252:1255	RESULTS In this work, a new compound, gallic acid 3-O-[6'-O-acetyl-β-D-glucoside], along with 16 known plant secondary metabolites (PSM) were isolated, characterized using UV, MS, and NMR (1H, 13C, DEPT, COSY, HMQC, and HMBC).
31095981	9	113	dep	NMR	1226:1228	arg1	13C					1235:1237	13C	1235:1237	13C	1235:1237	RESULTS In this work, a new compound, gallic acid 3-O-[6'-O-acetyl-β-D-glucoside], along with 16 known plant secondary metabolites (PSM) were isolated, characterized using UV, MS, and NMR (1H, 13C, DEPT, COSY, HMQC, and HMBC).
31095981	9	113	dep	NMR	1226:1228	arg1	DEPT					1240:1243	DEPT	1240:1243	DEPT	1240:1243	RESULTS In this work, a new compound, gallic acid 3-O-[6'-O-acetyl-β-D-glucoside], along with 16 known plant secondary metabolites (PSM) were isolated, characterized using UV, MS, and NMR (1H, 13C, DEPT, COSY, HMQC, and HMBC).
31095981	9	113	dep	NMR	1226:1228	arg1	HMBC					1262:1265	HMBC	1262:1265	HMBC	1262:1265	RESULTS In this work, a new compound, gallic acid 3-O-[6'-O-acetyl-β-D-glucoside], along with 16 known plant secondary metabolites (PSM) were isolated, characterized using UV, MS, and NMR (1H, 13C, DEPT, COSY, HMQC, and HMBC).
31095981	9	113	dep	NMR	1226:1228	arg1	1H					1231:1232	1H	1231:1232	1H	1231:1232	RESULTS In this work, a new compound, gallic acid 3-O-[6'-O-acetyl-β-D-glucoside], along with 16 known plant secondary metabolites (PSM) were isolated, characterized using UV, MS, and NMR (1H, 13C, DEPT, COSY, HMQC, and HMBC).
31095981	9	113	dep	NMR	1226:1228	arg1	COSY					1246:1249	COSY	1246:1249	COSY	1246:1249	RESULTS In this work, a new compound, gallic acid 3-O-[6'-O-acetyl-β-D-glucoside], along with 16 known plant secondary metabolites (PSM) were isolated, characterized using UV, MS, and NMR (1H, 13C, DEPT, COSY, HMQC, and HMBC).
31095981	13	114	theme	elevated	1965:1972	arg1	temperature					1981:1991	the elevated rectal temperature	1961:1991	the elevated rectal temperature in rats	1961:1999	Furthermore, adequate antipyretic effects were observed: The extract reduced the elevated rectal temperature in rats after intraperitoneal injection of Brewer's yeast.
31095981	8	115	theme	ani-inflammatory	960:975	arg1	activities					993:1002	The ani-inflammatory and antidibetic activities	956:1002	The ani-inflammatory and antidibetic activities	956:1002	The ani-inflammatory and antidibetic activities were studied in experimental animals.
31095981	7	116	theme	Antioxidant	891:901	arg1	effects					903:909	Antioxidant effects	891:909	Antioxidant effects	891:909	Antioxidant effects were determined in vitro and in HaCaT cells.
31095981	10	117	theme	Noticeable	1269:1278	arg1	effects					1292:1298	Noticeable antioxidant effects	1269:1298	Noticeable antioxidant effects	1269:1298	Noticeable antioxidant effects were determined in HaCaT cells: The extract reduced the elevated levels of ROS and p38 phosphorylation and increased the reduced glutathione (GSH) content induced by UVA.
31095981	5	118	theme	antidiabetic	719:730	arg1	activities					732:741	the antidiabetic activities	715:741	the antidiabetic activities	715:741	The molecular mode of action of the antidiabetic activities was also investigated.
31095981	8	119	theme	antidibetic	981:991	arg1	activities					993:1002	The ani-inflammatory and antidibetic activities	956:1002	The ani-inflammatory and antidibetic activities	956:1002	The ani-inflammatory and antidibetic activities were studied in experimental animals.
31095981	4	120	theme	analgesic	608:616	arg1	activities					648:657	the anti-inflammatory, analgesic, antipyretic and antidiabetic activities	585:657	the anti-inflammatory, analgesic, antipyretic and antidiabetic activities in relevant rat models	585:680	We also studied the anti-inflammatory, analgesic, antipyretic and antidiabetic activities in relevant rat models.
31095981	10	121	theme	HaCaT	1319:1323	arg1	cells					1325:1329	HaCaT cells	1319:1329	HaCaT cells	1319:1329	Noticeable antioxidant effects were determined in HaCaT cells: The extract reduced the elevated levels of ROS and p38 phosphorylation and increased the reduced glutathione (GSH) content induced by UVA.
31095981	14	122	with	concentration	2257:2269	arg1	potency					2292:2298	higher potency	2285:2298	higher potency than the positive control, glibenclamide	2285:2339	Moreover, the extract possessed robust anti-diabetic activity in streptozotocin (STZ) -diabetic rats: It markedly reduced the elevated serum glucose and lipid peroxidation levels and increased the insulin concentration in serum with higher potency than the positive control, glibenclamide.
31095981	13	123	theme	antipyretic	1906:1916	arg1	effects					1918:1924	adequate antipyretic effects	1897:1924	adequate antipyretic effects	1897:1924	Furthermore, adequate antipyretic effects were observed: The extract reduced the elevated rectal temperature in rats after intraperitoneal injection of Brewer's yeast.
31095981	2	124	theme	compounds	447:455	arg1	characterization					414:429	characterization	414:429	characterization of the isolated compounds	414:455	The aim of this study was to investigate the phytochemical composition of a methanol extract from leaves of E. uniflora and characterization of the isolated compounds.
31095981	13	125	theme	rectal	1974:1979	arg1	temperature					1981:1991	the elevated rectal temperature	1961:1991	the elevated rectal temperature in rats	1961:1999	Furthermore, adequate antipyretic effects were observed: The extract reduced the elevated rectal temperature in rats after intraperitoneal injection of Brewer's yeast.
31095981	0	126	theme	secondary	22:30	arg1	metabolites					32:42	secondary metabolites	22:42	secondary metabolites of Eugenia uniflora and their antioxidant, anti-inflammatory, pain killing and anti-diabetic activities	22:146	Chemical profiling of secondary metabolites of Eugenia uniflora and their antioxidant, anti-inflammatory, pain killing and anti-diabetic activities: A comprehensive approach.
31095981	12	127	theme	anti-nociceptive	1718:1733	arg1	properties					1735:1744	central and peripheral anti-nociceptive properties	1695:1744	central and peripheral anti-nociceptive properties	1695:1744	In rats, central and peripheral anti-nociceptive properties were also observed: The extract reduced the number of writhing in acid induced writhing and increased the latency time in hot plate test.
31095981	15	128	theme	binding	2561:2567	arg1	affinities					2569:2578	considerable binding affinities	2548:2578	considerable binding affinities	2548:2578	These effects might be associated with the interaction of PSM with the conserved amino acid residues of human pancreatic α-amylase (HPA), maltase glucoamylase (MGAM-C) and aldose reductase (ALR2) revealing considerable binding affinities.
31095981	0	129	theme	pain	106:109	arg1	killing					111:117	pain killing	106:117	pain killing	106:117	Chemical profiling of secondary metabolites of Eugenia uniflora and their antioxidant, anti-inflammatory, pain killing and anti-diabetic activities: A comprehensive approach.
31095981	0	130	theme	uniflora	55:62	arg1	metabolites					32:42	secondary metabolites	22:42	secondary metabolites of Eugenia uniflora and their antioxidant, anti-inflammatory, pain killing and anti-diabetic activities	22:146	Chemical profiling of secondary metabolites of Eugenia uniflora and their antioxidant, anti-inflammatory, pain killing and anti-diabetic activities: A comprehensive approach.
31095981	1	131	used	used	260:263	arg2	uniflora					240:247	Eugenia uniflora	232:247	Eugenia uniflora	232:247	ETHNOPHARMACOLOGICAL RELEVANCE The red Brazilian cherry, Eugenia uniflora, is widely used in traditional medicine.
31095981	1	131	used	used	260:263	arg2	RELEVANCE					196:204	ETHNOPHARMACOLOGICAL RELEVANCE	175:204	ETHNOPHARMACOLOGICAL RELEVANCE	175:204	ETHNOPHARMACOLOGICAL RELEVANCE The red Brazilian cherry, Eugenia uniflora, is widely used in traditional medicine.
31095981	16	132	theme	therapeutic	2739:2749	arg1	potential					2751:2759	solid therapeutic potential	2733:2759	solid therapeutic potential	2733:2759	CONCLUSION A plethora of substantial pharmacological properties indicates that Eugenia uniflora is a good antioxidant and a sustainable by-product with solid therapeutic potential for treating diabetes, inflammation, pain and related oxidative stress diseases.
31095981	1	133	dep	RELEVANCE	196:204	arg1	cherry					224:229	The red Brazilian cherry	206:229	The red Brazilian cherry	206:229	ETHNOPHARMACOLOGICAL RELEVANCE The red Brazilian cherry, Eugenia uniflora, is widely used in traditional medicine.
31095981	2	134	theme	phytochemical	335:347	arg1	composition					349:359	the phytochemical composition	331:359	the phytochemical composition of a methanol extract from leaves of E. uniflora and characterization of the isolated compounds	331:455	The aim of this study was to investigate the phytochemical composition of a methanol extract from leaves of E. uniflora and characterization of the isolated compounds.
31095981	15	135	theme	human	2446:2450	arg1	HPA					2474:2476	HPA	2474:2476	HPA	2474:2476	These effects might be associated with the interaction of PSM with the conserved amino acid residues of human pancreatic α-amylase (HPA), maltase glucoamylase (MGAM-C) and aldose reductase (ALR2) revealing considerable binding affinities.
31095981	15	135	theme	human	2446:2450	arg1	α-amylase					2463:2471	human pancreatic α-amylase	2446:2471	human pancreatic α-amylase (HPA)	2446:2477	These effects might be associated with the interaction of PSM with the conserved amino acid residues of human pancreatic α-amylase (HPA), maltase glucoamylase (MGAM-C) and aldose reductase (ALR2) revealing considerable binding affinities.
31095981	12	136	theme	plate	1872:1876	arg1	test					1878:1881	hot plate test	1868:1881	hot plate test	1868:1881	In rats, central and peripheral anti-nociceptive properties were also observed: The extract reduced the number of writhing in acid induced writhing and increased the latency time in hot plate test.
31095981	0	137	dep	profiling	9:17	arg1	approach					165:172	A comprehensive approach	149:172	Chemical profiling of secondary metabolites of Eugenia uniflora and their antioxidant, anti-inflammatory, pain killing and anti-diabetic activities: A comprehensive approach.	0:173	Chemical profiling of secondary metabolites of Eugenia uniflora and their antioxidant, anti-inflammatory, pain killing and anti-diabetic activities: A comprehensive approach.
31095981	10	138	theme	p38	1383:1385	arg1	levels					1365:1370	the elevated levels	1352:1370	the elevated levels of ROS and p38 phosphorylation	1352:1401	Noticeable antioxidant effects were determined in HaCaT cells: The extract reduced the elevated levels of ROS and p38 phosphorylation and increased the reduced glutathione (GSH) content induced by UVA.
31095981	9	139	theme	gallic	1080:1085	arg1	β-D-glucoside					1109:1121	gallic acid 3-O-[6'-O-acetyl-β-D-glucoside	1080:1121	gallic acid 3-O-[6'-O-acetyl-β-D-glucoside	1080:1121	RESULTS In this work, a new compound, gallic acid 3-O-[6'-O-acetyl-β-D-glucoside], along with 16 known plant secondary metabolites (PSM) were isolated, characterized using UV, MS, and NMR (1H, 13C, DEPT, COSY, HMQC, and HMBC).
31095981	2	140	theme	methanol	366:373	arg1	extract					375:381	a methanol extract	364:381	a methanol extract from leaves of E. uniflora and characterization of the isolated compounds	364:455	The aim of this study was to investigate the phytochemical composition of a methanol extract from leaves of E. uniflora and characterization of the isolated compounds.
31095981	15	141	theme	amino	2423:2427	arg1	α-amylase					2463:2471	human pancreatic α-amylase	2446:2471	human pancreatic α-amylase (HPA)	2446:2477	These effects might be associated with the interaction of PSM with the conserved amino acid residues of human pancreatic α-amylase (HPA), maltase glucoamylase (MGAM-C) and aldose reductase (ALR2) revealing considerable binding affinities.
31095981	15	141	theme	amino	2423:2427	arg1	glucoamylase					2488:2499	maltase glucoamylase	2480:2499	maltase glucoamylase (MGAM-C)	2480:2508	These effects might be associated with the interaction of PSM with the conserved amino acid residues of human pancreatic α-amylase (HPA), maltase glucoamylase (MGAM-C) and aldose reductase (ALR2) revealing considerable binding affinities.
31095981	15	141	theme	amino	2423:2427	arg1	reductase					2521:2529	aldose reductase	2514:2529	aldose reductase (ALR2)	2514:2536	These effects might be associated with the interaction of PSM with the conserved amino acid residues of human pancreatic α-amylase (HPA), maltase glucoamylase (MGAM-C) and aldose reductase (ALR2) revealing considerable binding affinities.
31095981	15	141	theme	amino	2423:2427	arg1	residues					2434:2441	the conserved amino acid residues	2409:2441	the conserved amino acid residues of human pancreatic α-amylase (HPA), maltase glucoamylase (MGAM-C) and aldose reductase (ALR2) revealing considerable binding affinities	2409:2578	These effects might be associated with the interaction of PSM with the conserved amino acid residues of human pancreatic α-amylase (HPA), maltase glucoamylase (MGAM-C) and aldose reductase (ALR2) revealing considerable binding affinities.
31095981	13	142	theme	yeast	2045:2049	arg1	injection					2023:2031	intraperitoneal injection	2007:2031	intraperitoneal injection of Brewer's yeast	2007:2049	Furthermore, adequate antipyretic effects were observed: The extract reduced the elevated rectal temperature in rats after intraperitoneal injection of Brewer's yeast.
30860530	4	0	theme	N-linked	1093:1100	arg1	glycopeptides					1102:1114	N-linked glycopeptides	1093:1114	N-linked glycopeptides	1093:1114	Benefiting from the judicious selection of the substrate, the abundance of binding sites, relatively high affinity between GSH and N-linked glycopeptides, and the multivalent interactions between N-linked glycopeptides and unoccupied surfaces of Ag NPs, this porous material showed great performance in N-linked glycopeptide enrichment.
30860530	6	1	theme	N-linked	1766:1773	arg1	glycopeptides					1775:1787	N-linked glycopeptides	1766:1787	N-linked glycopeptides from complex biological samples	1766:1819	It was also successfully applied to the enrichment of N-linked glycopeptides from complex biological samples.
30860530	5	2	theme	@	1604:1604	arg1	GSH					1608:1610	IgG/TpPa-1@Ag@GSH	1594:1610	IgG/TpPa-1@Ag@GSH	1594:1610	By enriching N-linked glycopeptides in tryptic digests of human serum immunoglobulin G (human IgG) followed by mass spectrometry analysis, our method was proved to have good sensitivity (1 fmol), high selectivity (1 : 1500, human IgG to bovine serum albumin), high binding capacity (160 mg g-1, IgG/TpPa-1@Ag@GSH), ultra-fast capture ability (only 1 min incubation time), and good reusability (at least 5 times).
30860530	3	3	theme	intermediate	657:668	arg1	products					670:677	TpPa-1@Ag@GSH and intermediate products	639:677	products	670:677	TpPa-1@Ag@GSH and intermediate products were confirmed and evaluated by nuclear magnetic resonance spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, transmission electron microscopy, scanning electron microscopy-energy dispersive spectroscopy, Brunauer-Emmett-Teller and thermogravimetric analyses.
30860530	2	4	theme	silver	391:396	arg1	nanoparticles					398:410	silver nanoparticles	391:410	silver nanoparticles (Ag NPs)	391:419	In this work, by utilizing the self-assembling of glutathione (GSH) on silver nanoparticles (Ag NPs), and the formation and dispersion of Ag NPs on a robust TpPa-1 substrate, a newly functionalized covalent organic framework (COF) called TpPa-1@Ag@GSH was synthesized via a simple two step post-synthetic modification.
30860530	2	4	theme	silver	391:396	arg1	NPs					416:418	Ag NPs	413:418	Ag NPs	413:418	In this work, by utilizing the self-assembling of glutathione (GSH) on silver nanoparticles (Ag NPs), and the formation and dispersion of Ag NPs on a robust TpPa-1 substrate, a newly functionalized covalent organic framework (COF) called TpPa-1@Ag@GSH was synthesized via a simple two step post-synthetic modification.
30860530	4	5	gly	glycopeptide	1274:1285	arg2	glycopeptide					1274:1285	N-linked glycopeptide enrichment	1265:1296	N-linked glycopeptide enrichment	1265:1296	Benefiting from the judicious selection of the substrate, the abundance of binding sites, relatively high affinity between GSH and N-linked glycopeptides, and the multivalent interactions between N-linked glycopeptides and unoccupied surfaces of Ag NPs, this porous material showed great performance in N-linked glycopeptide enrichment.
30860530	7	6	theme	facile	1974:1979	arg1	route					2032:2036	a very facile and easily popularized post-synthetic modification route	1967:2036	a very facile and easily popularized post-synthetic modification route for COFs	1967:2045	Our work improved the enrichment selectivity of COFs, reached the most rapid capture ability among off-column enrichment materials, and provided a very facile and easily popularized post-synthetic modification route for COFs in glycoproteome analysis.
30860530	6	7	from	samples	1813:1819	arg1	enrichment					1752:1761	the enrichment	1748:1761	the enrichment of N-linked glycopeptides from complex biological samples	1748:1819	It was also successfully applied to the enrichment of N-linked glycopeptides from complex biological samples.
30860530	6	7	from	samples	1813:1819	arg1	glycopeptides					1775:1787	N-linked glycopeptides	1766:1787	N-linked glycopeptides from complex biological samples	1766:1819	It was also successfully applied to the enrichment of N-linked glycopeptides from complex biological samples.
30860530	5	8	dep	proved	1453:1458	arg1	have					1463:1466	have	1463:1466	proved to have good sensitivity (1 fmol)	1453:1492	By enriching N-linked glycopeptides in tryptic digests of human serum immunoglobulin G (human IgG) followed by mass spectrometry analysis, our method was proved to have good sensitivity (1 fmol), high selectivity (1 : 1500, human IgG to bovine serum albumin), high binding capacity (160 mg g-1, IgG/TpPa-1@Ag@GSH), ultra-fast capture ability (only 1 min incubation time), and good reusability (at least 5 times).
30860530	2	9	theme	post-synthetic	610:623	arg1	modification					625:636	a simple two step post-synthetic modification	592:636	a simple two step post-synthetic modification	592:636	In this work, by utilizing the self-assembling of glutathione (GSH) on silver nanoparticles (Ag NPs), and the formation and dispersion of Ag NPs on a robust TpPa-1 substrate, a newly functionalized covalent organic framework (COF) called TpPa-1@Ag@GSH was synthesized via a simple two step post-synthetic modification.
30860530	5	10	theme	mg	1586:1587	arg1	capacity					1572:1579	high binding capacity	1559:1579	high binding capacity (160 mg g-1, IgG/TpPa-1@Ag@GSH)	1559:1611	By enriching N-linked glycopeptides in tryptic digests of human serum immunoglobulin G (human IgG) followed by mass spectrometry analysis, our method was proved to have good sensitivity (1 fmol), high selectivity (1 : 1500, human IgG to bovine serum albumin), high binding capacity (160 mg g-1, IgG/TpPa-1@Ag@GSH), ultra-fast capture ability (only 1 min incubation time), and good reusability (at least 5 times).
30860530	5	10	theme	mg	1586:1587	arg1	g-1					1589:1591	160 mg g-1	1582:1591	160 mg g-1	1582:1591	By enriching N-linked glycopeptides in tryptic digests of human serum immunoglobulin G (human IgG) followed by mass spectrometry analysis, our method was proved to have good sensitivity (1 fmol), high selectivity (1 : 1500, human IgG to bovine serum albumin), high binding capacity (160 mg g-1, IgG/TpPa-1@Ag@GSH), ultra-fast capture ability (only 1 min incubation time), and good reusability (at least 5 times).
30860530	5	11	theme	good	1468:1471	arg1	sensitivity					1473:1483	good sensitivity	1468:1483	good sensitivity (1 fmol)	1468:1492	By enriching N-linked glycopeptides in tryptic digests of human serum immunoglobulin G (human IgG) followed by mass spectrometry analysis, our method was proved to have good sensitivity (1 fmol), high selectivity (1 : 1500, human IgG to bovine serum albumin), high binding capacity (160 mg g-1, IgG/TpPa-1@Ag@GSH), ultra-fast capture ability (only 1 min incubation time), and good reusability (at least 5 times).
30860530	5	11	theme	good	1468:1471	arg1	fmol					1488:1491	1 fmol	1486:1491	1 fmol	1486:1491	By enriching N-linked glycopeptides in tryptic digests of human serum immunoglobulin G (human IgG) followed by mass spectrometry analysis, our method was proved to have good sensitivity (1 fmol), high selectivity (1 : 1500, human IgG to bovine serum albumin), high binding capacity (160 mg g-1, IgG/TpPa-1@Ag@GSH), ultra-fast capture ability (only 1 min incubation time), and good reusability (at least 5 times).
30860530	4	12	theme	Ag	1208:1209	arg1	NPs					1211:1213	Ag NPs	1208:1213	Ag NPs	1208:1213	Benefiting from the judicious selection of the substrate, the abundance of binding sites, relatively high affinity between GSH and N-linked glycopeptides, and the multivalent interactions between N-linked glycopeptides and unoccupied surfaces of Ag NPs, this porous material showed great performance in N-linked glycopeptide enrichment.
30860530	5	13	theme	ultra-fast	1614:1623	arg1	time					1664:1667	only 1 min incubation time	1642:1667	only 1 min incubation time	1642:1667	By enriching N-linked glycopeptides in tryptic digests of human serum immunoglobulin G (human IgG) followed by mass spectrometry analysis, our method was proved to have good sensitivity (1 fmol), high selectivity (1 : 1500, human IgG to bovine serum albumin), high binding capacity (160 mg g-1, IgG/TpPa-1@Ag@GSH), ultra-fast capture ability (only 1 min incubation time), and good reusability (at least 5 times).
30860530	5	13	theme	ultra-fast	1614:1623	arg1	ability					1633:1639	ultra-fast capture ability	1614:1639	ultra-fast capture ability (only 1 min incubation time)	1614:1668	By enriching N-linked glycopeptides in tryptic digests of human serum immunoglobulin G (human IgG) followed by mass spectrometry analysis, our method was proved to have good sensitivity (1 fmol), high selectivity (1 : 1500, human IgG to bovine serum albumin), high binding capacity (160 mg g-1, IgG/TpPa-1@Ag@GSH), ultra-fast capture ability (only 1 min incubation time), and good reusability (at least 5 times).
30860530	1	14	theme	efficient	233:241	arg1	enrichment					243:252	highly efficient enrichment	226:252	highly efficient enrichment of N-linked glycopeptides	226:278	The development of facilely synthetic materials for highly efficient enrichment of N-linked glycopeptides is essential in glycoproteome analysis.
30860530	2	15	theme	TpPa-1	558:563	arg1	GSH					568:570	called TpPa-1@Ag@GSH	551:570	called TpPa-1@Ag@GSH	551:570	In this work, by utilizing the self-assembling of glutathione (GSH) on silver nanoparticles (Ag NPs), and the formation and dispersion of Ag NPs on a robust TpPa-1 substrate, a newly functionalized covalent organic framework (COF) called TpPa-1@Ag@GSH was synthesized via a simple two step post-synthetic modification.
30860530	4	16	theme	N-linked	1158:1165	arg1	glycopeptides					1167:1179	N-linked glycopeptides	1158:1179	N-linked glycopeptides	1158:1179	Benefiting from the judicious selection of the substrate, the abundance of binding sites, relatively high affinity between GSH and N-linked glycopeptides, and the multivalent interactions between N-linked glycopeptides and unoccupied surfaces of Ag NPs, this porous material showed great performance in N-linked glycopeptide enrichment.
30860530	3	17	theme	magnetic	719:726	arg1	resonance					728:736	nuclear magnetic resonance	711:736	nuclear magnetic resonance spectroscopy	711:749	TpPa-1@Ag@GSH and intermediate products were confirmed and evaluated by nuclear magnetic resonance spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, transmission electron microscopy, scanning electron microscopy-energy dispersive spectroscopy, Brunauer-Emmett-Teller and thermogravimetric analyses.
30860530	0	18	theme	N-linked	150:157	arg1	glycopeptides					159:171	N-linked glycopeptides	150:171	N-linked glycopeptides	150:171	A facilely synthesized glutathione-functionalized silver nanoparticle-grafted covalent organic framework for rapid and highly efficient enrichment of N-linked glycopeptides.
30860530	5	19	dep	1 	1513:1514	arg1	 1500					1516:1520	 1500	1516:1520	 1500	1516:1520	By enriching N-linked glycopeptides in tryptic digests of human serum immunoglobulin G (human IgG) followed by mass spectrometry analysis, our method was proved to have good sensitivity (1 fmol), high selectivity (1 : 1500, human IgG to bovine serum albumin), high binding capacity (160 mg g-1, IgG/TpPa-1@Ag@GSH), ultra-fast capture ability (only 1 min incubation time), and good reusability (at least 5 times).
30860530	5	19	dep	1 	1513:1514	arg1	IgG					1529:1531	human IgG	1523:1531	human IgG to bovine serum albumin	1523:1555	By enriching N-linked glycopeptides in tryptic digests of human serum immunoglobulin G (human IgG) followed by mass spectrometry analysis, our method was proved to have good sensitivity (1 fmol), high selectivity (1 : 1500, human IgG to bovine serum albumin), high binding capacity (160 mg g-1, IgG/TpPa-1@Ag@GSH), ultra-fast capture ability (only 1 min incubation time), and good reusability (at least 5 times).
30860530	5	20	theme	@	1607:1607	arg1	GSH					1608:1610	IgG/TpPa-1@Ag@GSH	1594:1610	IgG/TpPa-1@Ag@GSH	1594:1610	By enriching N-linked glycopeptides in tryptic digests of human serum immunoglobulin G (human IgG) followed by mass spectrometry analysis, our method was proved to have good sensitivity (1 fmol), high selectivity (1 : 1500, human IgG to bovine serum albumin), high binding capacity (160 mg g-1, IgG/TpPa-1@Ag@GSH), ultra-fast capture ability (only 1 min incubation time), and good reusability (at least 5 times).
30860530	3	21	theme	infrared	770:777	arg1	spectroscopy					779:790	infrared spectroscopy	770:790	infrared spectroscopy	770:790	TpPa-1@Ag@GSH and intermediate products were confirmed and evaluated by nuclear magnetic resonance spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, transmission electron microscopy, scanning electron microscopy-energy dispersive spectroscopy, Brunauer-Emmett-Teller and thermogravimetric analyses.
30860530	1	22	theme	glycopeptides	266:278	arg1	enrichment					243:252	highly efficient enrichment	226:252	highly efficient enrichment of N-linked glycopeptides	226:278	The development of facilely synthetic materials for highly efficient enrichment of N-linked glycopeptides is essential in glycoproteome analysis.
30860530	5	23	dep	selectivity	1500:1510	arg1	1 					1513:1514	1 	1513:1514	1 	1513:1514	By enriching N-linked glycopeptides in tryptic digests of human serum immunoglobulin G (human IgG) followed by mass spectrometry analysis, our method was proved to have good sensitivity (1 fmol), high selectivity (1 : 1500, human IgG to bovine serum albumin), high binding capacity (160 mg g-1, IgG/TpPa-1@Ag@GSH), ultra-fast capture ability (only 1 min incubation time), and good reusability (at least 5 times).
30860530	5	24	from	glycopeptides	1321:1333	arg1	digests					1346:1352	tryptic digests	1338:1352	tryptic digests of human serum immunoglobulin G (human IgG)	1338:1396	By enriching N-linked glycopeptides in tryptic digests of human serum immunoglobulin G (human IgG) followed by mass spectrometry analysis, our method was proved to have good sensitivity (1 fmol), high selectivity (1 : 1500, human IgG to bovine serum albumin), high binding capacity (160 mg g-1, IgG/TpPa-1@Ag@GSH), ultra-fast capture ability (only 1 min incubation time), and good reusability (at least 5 times).
30860530	0	25	gly	glycopeptides	159:171	arg2	glycopeptides					159:171	N-linked glycopeptides	150:171	N-linked glycopeptides	150:171	A facilely synthesized glutathione-functionalized silver nanoparticle-grafted covalent organic framework for rapid and highly efficient enrichment of N-linked glycopeptides.
30860530	3	26	theme	microscopy-energy	864:880	arg1	spectroscopy					893:904	scanning electron microscopy-energy dispersive spectroscopy	846:904	scanning electron microscopy-energy dispersive spectroscopy	846:904	TpPa-1@Ag@GSH and intermediate products were confirmed and evaluated by nuclear magnetic resonance spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, transmission electron microscopy, scanning electron microscopy-energy dispersive spectroscopy, Brunauer-Emmett-Teller and thermogravimetric analyses.
30860530	2	27	theme	Ag	565:566	arg1	GSH					568:570	called TpPa-1@Ag@GSH	551:570	called TpPa-1@Ag@GSH	551:570	In this work, by utilizing the self-assembling of glutathione (GSH) on silver nanoparticles (Ag NPs), and the formation and dispersion of Ag NPs on a robust TpPa-1 substrate, a newly functionalized covalent organic framework (COF) called TpPa-1@Ag@GSH was synthesized via a simple two step post-synthetic modification.
30860530	7	28	theme	glycoproteome	2050:2062	arg1	analysis					2064:2071	glycoproteome analysis	2050:2071	glycoproteome analysis	2050:2071	Our work improved the enrichment selectivity of COFs, reached the most rapid capture ability among off-column enrichment materials, and provided a very facile and easily popularized post-synthetic modification route for COFs in glycoproteome analysis.
30860530	5	29	theme	serum	1363:1367	arg1	IgG					1393:1395	human IgG	1387:1395	human IgG	1387:1395	By enriching N-linked glycopeptides in tryptic digests of human serum immunoglobulin G (human IgG) followed by mass spectrometry analysis, our method was proved to have good sensitivity (1 fmol), high selectivity (1 : 1500, human IgG to bovine serum albumin), high binding capacity (160 mg g-1, IgG/TpPa-1@Ag@GSH), ultra-fast capture ability (only 1 min incubation time), and good reusability (at least 5 times).
30860530	5	29	theme	serum	1363:1367	arg1	G					1384:1384	human serum immunoglobulin G	1357:1384	human serum immunoglobulin G (human IgG)	1357:1396	By enriching N-linked glycopeptides in tryptic digests of human serum immunoglobulin G (human IgG) followed by mass spectrometry analysis, our method was proved to have good sensitivity (1 fmol), high selectivity (1 : 1500, human IgG to bovine serum albumin), high binding capacity (160 mg g-1, IgG/TpPa-1@Ag@GSH), ultra-fast capture ability (only 1 min incubation time), and good reusability (at least 5 times).
30860530	1	30	from	essential	283:291	arg1	analysis					310:317	glycoproteome analysis	296:317	glycoproteome analysis	296:317	The development of facilely synthetic materials for highly efficient enrichment of N-linked glycopeptides is essential in glycoproteome analysis.
30860530	3	31	theme	scanning	846:853	arg1	spectroscopy					893:904	scanning electron microscopy-energy dispersive spectroscopy	846:904	scanning electron microscopy-energy dispersive spectroscopy	846:904	TpPa-1@Ag@GSH and intermediate products were confirmed and evaluated by nuclear magnetic resonance spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, transmission electron microscopy, scanning electron microscopy-energy dispersive spectroscopy, Brunauer-Emmett-Teller and thermogravimetric analyses.
30860530	2	32	theme	functionalized	503:516	arg1	COF					546:548	COF	546:548	COF	546:548	In this work, by utilizing the self-assembling of glutathione (GSH) on silver nanoparticles (Ag NPs), and the formation and dispersion of Ag NPs on a robust TpPa-1 substrate, a newly functionalized covalent organic framework (COF) called TpPa-1@Ag@GSH was synthesized via a simple two step post-synthetic modification.
30860530	2	32	theme	functionalized	503:516	arg1	framework					535:543	a newly functionalized covalent organic framework	495:543	a newly functionalized covalent organic framework (COF)	495:549	In this work, by utilizing the self-assembling of glutathione (GSH) on silver nanoparticles (Ag NPs), and the formation and dispersion of Ag NPs on a robust TpPa-1 substrate, a newly functionalized covalent organic framework (COF) called TpPa-1@Ag@GSH was synthesized via a simple two step post-synthetic modification.
30860530	2	32	theme	functionalized	503:516	arg1	substrate					484:492	a robust TpPa-1 substrate	468:492	a robust TpPa-1 substrate	468:492	In this work, by utilizing the self-assembling of glutathione (GSH) on silver nanoparticles (Ag NPs), and the formation and dispersion of Ag NPs on a robust TpPa-1 substrate, a newly functionalized covalent organic framework (COF) called TpPa-1@Ag@GSH was synthesized via a simple two step post-synthetic modification.
30860530	5	33	theme	G	1384:1384	arg1	digests					1346:1352	tryptic digests	1338:1352	tryptic digests of human serum immunoglobulin G (human IgG)	1338:1396	By enriching N-linked glycopeptides in tryptic digests of human serum immunoglobulin G (human IgG) followed by mass spectrometry analysis, our method was proved to have good sensitivity (1 fmol), high selectivity (1 : 1500, human IgG to bovine serum albumin), high binding capacity (160 mg g-1, IgG/TpPa-1@Ag@GSH), ultra-fast capture ability (only 1 min incubation time), and good reusability (at least 5 times).
30860530	4	34	theme	binding	1037:1043	arg1	sites					1045:1049	binding sites	1037:1049	binding sites	1037:1049	Benefiting from the judicious selection of the substrate, the abundance of binding sites, relatively high affinity between GSH and N-linked glycopeptides, and the multivalent interactions between N-linked glycopeptides and unoccupied surfaces of Ag NPs, this porous material showed great performance in N-linked glycopeptide enrichment.
30860530	1	35	link	N-linked	257:264	arg1	glycopeptides					266:278	N-linked glycopeptides	257:278	N-linked glycopeptides	257:278	The development of facilely synthetic materials for highly efficient enrichment of N-linked glycopeptides is essential in glycoproteome analysis.
30860530	4	36	theme	porous	1221:1226	arg1	material					1228:1235	this porous material	1216:1235	this porous material	1216:1235	Benefiting from the judicious selection of the substrate, the abundance of binding sites, relatively high affinity between GSH and N-linked glycopeptides, and the multivalent interactions between N-linked glycopeptides and unoccupied surfaces of Ag NPs, this porous material showed great performance in N-linked glycopeptide enrichment.
30860530	5	37	theme	tryptic	1338:1344	arg1	digests					1346:1352	tryptic digests	1338:1352	tryptic digests of human serum immunoglobulin G (human IgG)	1338:1396	By enriching N-linked glycopeptides in tryptic digests of human serum immunoglobulin G (human IgG) followed by mass spectrometry analysis, our method was proved to have good sensitivity (1 fmol), high selectivity (1 : 1500, human IgG to bovine serum albumin), high binding capacity (160 mg g-1, IgG/TpPa-1@Ag@GSH), ultra-fast capture ability (only 1 min incubation time), and good reusability (at least 5 times).
30860530	0	38	theme	rapid	109:113	arg1	enrichment					136:145	rapid and highly efficient enrichment	109:145	rapid and highly efficient enrichment of N-linked glycopeptides	109:171	A facilely synthesized glutathione-functionalized silver nanoparticle-grafted covalent organic framework for rapid and highly efficient enrichment of N-linked glycopeptides.
30860530	7	39	theme	capture	1899:1905	arg1	ability					1907:1913	the most rapid capture ability	1884:1913	the most rapid capture ability	1884:1913	Our work improved the enrichment selectivity of COFs, reached the most rapid capture ability among off-column enrichment materials, and provided a very facile and easily popularized post-synthetic modification route for COFs in glycoproteome analysis.
30860530	1	40	theme	synthetic	202:210	arg1	materials					212:220	facilely synthetic materials	193:220	facilely synthetic materials for highly efficient enrichment of N-linked glycopeptides	193:278	The development of facilely synthetic materials for highly efficient enrichment of N-linked glycopeptides is essential in glycoproteome analysis.
30860530	2	41	from	glutathione	370:380	arg1	nanoparticles					398:410	silver nanoparticles	391:410	silver nanoparticles (Ag NPs)	391:419	In this work, by utilizing the self-assembling of glutathione (GSH) on silver nanoparticles (Ag NPs), and the formation and dispersion of Ag NPs on a robust TpPa-1 substrate, a newly functionalized covalent organic framework (COF) called TpPa-1@Ag@GSH was synthesized via a simple two step post-synthetic modification.
30860530	2	41	from	glutathione	370:380	arg1	NPs					416:418	Ag NPs	413:418	Ag NPs	413:418	In this work, by utilizing the self-assembling of glutathione (GSH) on silver nanoparticles (Ag NPs), and the formation and dispersion of Ag NPs on a robust TpPa-1 substrate, a newly functionalized covalent organic framework (COF) called TpPa-1@Ag@GSH was synthesized via a simple two step post-synthetic modification.
30860530	5	42	theme	human	1523:1527	arg1	IgG					1529:1531	human IgG	1523:1531	human IgG to bovine serum albumin	1523:1555	By enriching N-linked glycopeptides in tryptic digests of human serum immunoglobulin G (human IgG) followed by mass spectrometry analysis, our method was proved to have good sensitivity (1 fmol), high selectivity (1 : 1500, human IgG to bovine serum albumin), high binding capacity (160 mg g-1, IgG/TpPa-1@Ag@GSH), ultra-fast capture ability (only 1 min incubation time), and good reusability (at least 5 times).
30860530	4	43	link	N-linked	1265:1272	arg1	enrichment					1287:1296	N-linked glycopeptide enrichment	1265:1296	N-linked glycopeptide enrichment	1265:1296	Benefiting from the judicious selection of the substrate, the abundance of binding sites, relatively high affinity between GSH and N-linked glycopeptides, and the multivalent interactions between N-linked glycopeptides and unoccupied surfaces of Ag NPs, this porous material showed great performance in N-linked glycopeptide enrichment.
30860530	3	44	theme	transmission	812:823	arg1	microscopy					834:843	transmission electron microscopy	812:843	transmission electron microscopy	812:843	TpPa-1@Ag@GSH and intermediate products were confirmed and evaluated by nuclear magnetic resonance spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, transmission electron microscopy, scanning electron microscopy-energy dispersive spectroscopy, Brunauer-Emmett-Teller and thermogravimetric analyses.
30860530	3	45	dep	Fourier	752:758	arg1	transform					760:768	transform	760:768	transform infrared spectroscopy	760:790	TpPa-1@Ag@GSH and intermediate products were confirmed and evaluated by nuclear magnetic resonance spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, transmission electron microscopy, scanning electron microscopy-energy dispersive spectroscopy, Brunauer-Emmett-Teller and thermogravimetric analyses.
30860530	2	46	theme	organic	527:533	arg1	COF					546:548	COF	546:548	COF	546:548	In this work, by utilizing the self-assembling of glutathione (GSH) on silver nanoparticles (Ag NPs), and the formation and dispersion of Ag NPs on a robust TpPa-1 substrate, a newly functionalized covalent organic framework (COF) called TpPa-1@Ag@GSH was synthesized via a simple two step post-synthetic modification.
30860530	2	46	theme	organic	527:533	arg1	framework					535:543	a newly functionalized covalent organic framework	495:543	a newly functionalized covalent organic framework (COF)	495:549	In this work, by utilizing the self-assembling of glutathione (GSH) on silver nanoparticles (Ag NPs), and the formation and dispersion of Ag NPs on a robust TpPa-1 substrate, a newly functionalized covalent organic framework (COF) called TpPa-1@Ag@GSH was synthesized via a simple two step post-synthetic modification.
30860530	2	46	theme	organic	527:533	arg1	substrate					484:492	a robust TpPa-1 substrate	468:492	a robust TpPa-1 substrate	468:492	In this work, by utilizing the self-assembling of glutathione (GSH) on silver nanoparticles (Ag NPs), and the formation and dispersion of Ag NPs on a robust TpPa-1 substrate, a newly functionalized covalent organic framework (COF) called TpPa-1@Ag@GSH was synthesized via a simple two step post-synthetic modification.
30860530	5	47	theme	incubation	1653:1662	arg1	time					1664:1667	only 1 min incubation time	1642:1667	only 1 min incubation time	1642:1667	By enriching N-linked glycopeptides in tryptic digests of human serum immunoglobulin G (human IgG) followed by mass spectrometry analysis, our method was proved to have good sensitivity (1 fmol), high selectivity (1 : 1500, human IgG to bovine serum albumin), high binding capacity (160 mg g-1, IgG/TpPa-1@Ag@GSH), ultra-fast capture ability (only 1 min incubation time), and good reusability (at least 5 times).
30860530	5	47	theme	incubation	1653:1662	arg1	ability					1633:1639	ultra-fast capture ability	1614:1639	ultra-fast capture ability (only 1 min incubation time)	1614:1668	By enriching N-linked glycopeptides in tryptic digests of human serum immunoglobulin G (human IgG) followed by mass spectrometry analysis, our method was proved to have good sensitivity (1 fmol), high selectivity (1 : 1500, human IgG to bovine serum albumin), high binding capacity (160 mg g-1, IgG/TpPa-1@Ag@GSH), ultra-fast capture ability (only 1 min incubation time), and good reusability (at least 5 times).
30860530	3	48	theme	thermogravimetric	934:950	arg1	analyses					952:959	thermogravimetric analyses	934:959	thermogravimetric analyses	934:959	TpPa-1@Ag@GSH and intermediate products were confirmed and evaluated by nuclear magnetic resonance spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, transmission electron microscopy, scanning electron microscopy-energy dispersive spectroscopy, Brunauer-Emmett-Teller and thermogravimetric analyses.
30860530	4	49	theme	N-linked	1265:1272	arg1	enrichment					1287:1296	N-linked glycopeptide enrichment	1265:1296	N-linked glycopeptide enrichment	1265:1296	Benefiting from the judicious selection of the substrate, the abundance of binding sites, relatively high affinity between GSH and N-linked glycopeptides, and the multivalent interactions between N-linked glycopeptides and unoccupied surfaces of Ag NPs, this porous material showed great performance in N-linked glycopeptide enrichment.
30860530	5	50	theme	spectrometry	1415:1426	arg1	analysis					1428:1435	mass spectrometry analysis	1410:1435	mass spectrometry analysis	1410:1435	By enriching N-linked glycopeptides in tryptic digests of human serum immunoglobulin G (human IgG) followed by mass spectrometry analysis, our method was proved to have good sensitivity (1 fmol), high selectivity (1 : 1500, human IgG to bovine serum albumin), high binding capacity (160 mg g-1, IgG/TpPa-1@Ag@GSH), ultra-fast capture ability (only 1 min incubation time), and good reusability (at least 5 times).
30860530	7	51	theme	COFs	1870:1873	arg1	selectivity					1855:1865	the enrichment selectivity	1840:1865	the enrichment selectivity of COFs	1840:1873	Our work improved the enrichment selectivity of COFs, reached the most rapid capture ability among off-column enrichment materials, and provided a very facile and easily popularized post-synthetic modification route for COFs in glycoproteome analysis.
30860530	6	52	from	enrichment	1752:1761	arg1	samples					1813:1819	complex biological samples	1794:1819	complex biological samples	1794:1819	It was also successfully applied to the enrichment of N-linked glycopeptides from complex biological samples.
30860530	2	53	theme	Ag	458:459	arg1	NPs					461:463	Ag NPs	458:463	Ag NPs	458:463	In this work, by utilizing the self-assembling of glutathione (GSH) on silver nanoparticles (Ag NPs), and the formation and dispersion of Ag NPs on a robust TpPa-1 substrate, a newly functionalized covalent organic framework (COF) called TpPa-1@Ag@GSH was synthesized via a simple two step post-synthetic modification.
30860530	5	54	theme	human	1387:1391	arg1	IgG					1393:1395	human IgG	1387:1395	human IgG	1387:1395	By enriching N-linked glycopeptides in tryptic digests of human serum immunoglobulin G (human IgG) followed by mass spectrometry analysis, our method was proved to have good sensitivity (1 fmol), high selectivity (1 : 1500, human IgG to bovine serum albumin), high binding capacity (160 mg g-1, IgG/TpPa-1@Ag@GSH), ultra-fast capture ability (only 1 min incubation time), and good reusability (at least 5 times).
30860530	5	54	theme	human	1387:1391	arg1	G					1384:1384	human serum immunoglobulin G	1357:1384	human serum immunoglobulin G (human IgG)	1357:1396	By enriching N-linked glycopeptides in tryptic digests of human serum immunoglobulin G (human IgG) followed by mass spectrometry analysis, our method was proved to have good sensitivity (1 fmol), high selectivity (1 : 1500, human IgG to bovine serum albumin), high binding capacity (160 mg g-1, IgG/TpPa-1@Ag@GSH), ultra-fast capture ability (only 1 min incubation time), and good reusability (at least 5 times).
30860530	0	55	theme	synthesized	11:21	arg1	silver					50:55	A facilely synthesized glutathione-functionalized silver	0:55	A facilely synthesized glutathione-functionalized silver	0:55	A facilely synthesized glutathione-functionalized silver nanoparticle-grafted covalent organic framework for rapid and highly efficient enrichment of N-linked glycopeptides.
30860530	5	56	theme	high	1559:1562	arg1	capacity					1572:1579	high binding capacity	1559:1579	high binding capacity (160 mg g-1, IgG/TpPa-1@Ag@GSH)	1559:1611	By enriching N-linked glycopeptides in tryptic digests of human serum immunoglobulin G (human IgG) followed by mass spectrometry analysis, our method was proved to have good sensitivity (1 fmol), high selectivity (1 : 1500, human IgG to bovine serum albumin), high binding capacity (160 mg g-1, IgG/TpPa-1@Ag@GSH), ultra-fast capture ability (only 1 min incubation time), and good reusability (at least 5 times).
30860530	5	56	theme	high	1559:1562	arg1	g-1					1589:1591	160 mg g-1	1582:1591	160 mg g-1	1582:1591	By enriching N-linked glycopeptides in tryptic digests of human serum immunoglobulin G (human IgG) followed by mass spectrometry analysis, our method was proved to have good sensitivity (1 fmol), high selectivity (1 : 1500, human IgG to bovine serum albumin), high binding capacity (160 mg g-1, IgG/TpPa-1@Ag@GSH), ultra-fast capture ability (only 1 min incubation time), and good reusability (at least 5 times).
30860530	3	57	theme	@	645:645	arg1	GSH					649:651	TpPa-1@Ag@GSH and intermediate products	639:677	GSH	649:651	TpPa-1@Ag@GSH and intermediate products were confirmed and evaluated by nuclear magnetic resonance spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, transmission electron microscopy, scanning electron microscopy-energy dispersive spectroscopy, Brunauer-Emmett-Teller and thermogravimetric analyses.
30860530	2	58	dep	formation	430:438	arg1	GSH					568:570	called TpPa-1@Ag@GSH	551:570	called TpPa-1@Ag@GSH	551:570	In this work, by utilizing the self-assembling of glutathione (GSH) on silver nanoparticles (Ag NPs), and the formation and dispersion of Ag NPs on a robust TpPa-1 substrate, a newly functionalized covalent organic framework (COF) called TpPa-1@Ag@GSH was synthesized via a simple two step post-synthetic modification.
30860530	4	59	theme	sites	1045:1049	arg1	abundance					1024:1032	the abundance	1020:1032	the abundance of binding sites	1020:1049	Benefiting from the judicious selection of the substrate, the abundance of binding sites, relatively high affinity between GSH and N-linked glycopeptides, and the multivalent interactions between N-linked glycopeptides and unoccupied surfaces of Ag NPs, this porous material showed great performance in N-linked glycopeptide enrichment.
30860530	4	59	theme	sites	1045:1049	arg1	interactions					1137:1148	the multivalent interactions	1121:1148	the multivalent interactions between N-linked glycopeptides and unoccupied surfaces of Ag NPs	1121:1213	Benefiting from the judicious selection of the substrate, the abundance of binding sites, relatively high affinity between GSH and N-linked glycopeptides, and the multivalent interactions between N-linked glycopeptides and unoccupied surfaces of Ag NPs, this porous material showed great performance in N-linked glycopeptide enrichment.
30860530	4	59	theme	sites	1045:1049	arg1	affinity					1068:1075	relatively high affinity	1052:1075	relatively high affinity between GSH and N-linked glycopeptides	1052:1114	Benefiting from the judicious selection of the substrate, the abundance of binding sites, relatively high affinity between GSH and N-linked glycopeptides, and the multivalent interactions between N-linked glycopeptides and unoccupied surfaces of Ag NPs, this porous material showed great performance in N-linked glycopeptide enrichment.
30860530	4	59	theme	sites	1045:1049	arg1	selection					992:1000	the judicious selection	978:1000	the judicious selection of the substrate	978:1017	Benefiting from the judicious selection of the substrate, the abundance of binding sites, relatively high affinity between GSH and N-linked glycopeptides, and the multivalent interactions between N-linked glycopeptides and unoccupied surfaces of Ag NPs, this porous material showed great performance in N-linked glycopeptide enrichment.
30860530	4	60	from	performance	1250:1260	arg1	enrichment					1287:1296	N-linked glycopeptide enrichment	1265:1296	N-linked glycopeptide enrichment	1265:1296	Benefiting from the judicious selection of the substrate, the abundance of binding sites, relatively high affinity between GSH and N-linked glycopeptides, and the multivalent interactions between N-linked glycopeptides and unoccupied surfaces of Ag NPs, this porous material showed great performance in N-linked glycopeptide enrichment.
30860530	4	61	theme	high	1063:1066	arg1	affinity					1068:1075	relatively high affinity	1052:1075	relatively high affinity between GSH and N-linked glycopeptides	1052:1114	Benefiting from the judicious selection of the substrate, the abundance of binding sites, relatively high affinity between GSH and N-linked glycopeptides, and the multivalent interactions between N-linked glycopeptides and unoccupied surfaces of Ag NPs, this porous material showed great performance in N-linked glycopeptide enrichment.
30860530	0	62	theme	covalent	78:85	arg1	framework					95:103	covalent organic framework	78:103	covalent organic framework for rapid and highly efficient enrichment of N-linked glycopeptides	78:171	A facilely synthesized glutathione-functionalized silver nanoparticle-grafted covalent organic framework for rapid and highly efficient enrichment of N-linked glycopeptides.
30860530	4	63	theme	multivalent	1125:1135	arg1	interactions					1137:1148	the multivalent interactions	1121:1148	the multivalent interactions between N-linked glycopeptides and unoccupied surfaces of Ag NPs	1121:1213	Benefiting from the judicious selection of the substrate, the abundance of binding sites, relatively high affinity between GSH and N-linked glycopeptides, and the multivalent interactions between N-linked glycopeptides and unoccupied surfaces of Ag NPs, this porous material showed great performance in N-linked glycopeptide enrichment.
30860530	5	64	theme	serum	1543:1547	arg1	albumin					1549:1555	bovine serum albumin	1536:1555	bovine serum albumin	1536:1555	By enriching N-linked glycopeptides in tryptic digests of human serum immunoglobulin G (human IgG) followed by mass spectrometry analysis, our method was proved to have good sensitivity (1 fmol), high selectivity (1 : 1500, human IgG to bovine serum albumin), high binding capacity (160 mg g-1, IgG/TpPa-1@Ag@GSH), ultra-fast capture ability (only 1 min incubation time), and good reusability (at least 5 times).
30860530	2	65	from	formation	430:438	arg1	framework					535:543	a newly functionalized covalent organic framework	495:543	a newly functionalized covalent organic framework (COF)	495:549	In this work, by utilizing the self-assembling of glutathione (GSH) on silver nanoparticles (Ag NPs), and the formation and dispersion of Ag NPs on a robust TpPa-1 substrate, a newly functionalized covalent organic framework (COF) called TpPa-1@Ag@GSH was synthesized via a simple two step post-synthetic modification.
30860530	2	65	from	formation	430:438	arg1	substrate					484:492	a robust TpPa-1 substrate	468:492	a robust TpPa-1 substrate	468:492	In this work, by utilizing the self-assembling of glutathione (GSH) on silver nanoparticles (Ag NPs), and the formation and dispersion of Ag NPs on a robust TpPa-1 substrate, a newly functionalized covalent organic framework (COF) called TpPa-1@Ag@GSH was synthesized via a simple two step post-synthetic modification.
30860530	6	66	theme	complex	1794:1800	arg1	samples					1813:1819	complex biological samples	1794:1819	complex biological samples	1794:1819	It was also successfully applied to the enrichment of N-linked glycopeptides from complex biological samples.
30860530	3	67	theme	@	648:648	arg1	GSH					649:651	TpPa-1@Ag@GSH and intermediate products	639:677	GSH	649:651	TpPa-1@Ag@GSH and intermediate products were confirmed and evaluated by nuclear magnetic resonance spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, transmission electron microscopy, scanning electron microscopy-energy dispersive spectroscopy, Brunauer-Emmett-Teller and thermogravimetric analyses.
30860530	2	68	theme	Ag	413:414	arg1	nanoparticles					398:410	silver nanoparticles	391:410	silver nanoparticles (Ag NPs)	391:419	In this work, by utilizing the self-assembling of glutathione (GSH) on silver nanoparticles (Ag NPs), and the formation and dispersion of Ag NPs on a robust TpPa-1 substrate, a newly functionalized covalent organic framework (COF) called TpPa-1@Ag@GSH was synthesized via a simple two step post-synthetic modification.
30860530	2	68	theme	Ag	413:414	arg1	NPs					416:418	Ag NPs	413:418	Ag NPs	413:418	In this work, by utilizing the self-assembling of glutathione (GSH) on silver nanoparticles (Ag NPs), and the formation and dispersion of Ag NPs on a robust TpPa-1 substrate, a newly functionalized covalent organic framework (COF) called TpPa-1@Ag@GSH was synthesized via a simple two step post-synthetic modification.
30860530	5	69	theme	N-linked	1312:1319	arg1	glycopeptides					1321:1333	N-linked glycopeptides	1312:1333	N-linked glycopeptides in tryptic digests of human serum immunoglobulin G (human IgG) followed by mass spectrometry analysis	1312:1435	By enriching N-linked glycopeptides in tryptic digests of human serum immunoglobulin G (human IgG) followed by mass spectrometry analysis, our method was proved to have good sensitivity (1 fmol), high selectivity (1 : 1500, human IgG to bovine serum albumin), high binding capacity (160 mg g-1, IgG/TpPa-1@Ag@GSH), ultra-fast capture ability (only 1 min incubation time), and good reusability (at least 5 times).
30860530	2	70	theme	robust	470:475	arg1	framework					535:543	a newly functionalized covalent organic framework	495:543	a newly functionalized covalent organic framework (COF)	495:549	In this work, by utilizing the self-assembling of glutathione (GSH) on silver nanoparticles (Ag NPs), and the formation and dispersion of Ag NPs on a robust TpPa-1 substrate, a newly functionalized covalent organic framework (COF) called TpPa-1@Ag@GSH was synthesized via a simple two step post-synthetic modification.
30860530	2	70	theme	robust	470:475	arg1	substrate					484:492	a robust TpPa-1 substrate	468:492	a robust TpPa-1 substrate	468:492	In this work, by utilizing the self-assembling of glutathione (GSH) on silver nanoparticles (Ag NPs), and the formation and dispersion of Ag NPs on a robust TpPa-1 substrate, a newly functionalized covalent organic framework (COF) called TpPa-1@Ag@GSH was synthesized via a simple two step post-synthetic modification.
30860530	2	71	from	dispersion	444:453	arg1	framework					535:543	a newly functionalized covalent organic framework	495:543	a newly functionalized covalent organic framework (COF)	495:549	In this work, by utilizing the self-assembling of glutathione (GSH) on silver nanoparticles (Ag NPs), and the formation and dispersion of Ag NPs on a robust TpPa-1 substrate, a newly functionalized covalent organic framework (COF) called TpPa-1@Ag@GSH was synthesized via a simple two step post-synthetic modification.
30860530	2	71	from	dispersion	444:453	arg1	substrate					484:492	a robust TpPa-1 substrate	468:492	a robust TpPa-1 substrate	468:492	In this work, by utilizing the self-assembling of glutathione (GSH) on silver nanoparticles (Ag NPs), and the formation and dispersion of Ag NPs on a robust TpPa-1 substrate, a newly functionalized covalent organic framework (COF) called TpPa-1@Ag@GSH was synthesized via a simple two step post-synthetic modification.
30860530	6	72	theme	glycopeptides	1775:1787	arg1	enrichment					1752:1761	the enrichment	1748:1761	the enrichment of N-linked glycopeptides from complex biological samples	1748:1819	It was also successfully applied to the enrichment of N-linked glycopeptides from complex biological samples.
30860530	5	73	theme	IgG/TpPa-1	1594:1603	arg1	GSH					1608:1610	IgG/TpPa-1@Ag@GSH	1594:1610	IgG/TpPa-1@Ag@GSH	1594:1610	By enriching N-linked glycopeptides in tryptic digests of human serum immunoglobulin G (human IgG) followed by mass spectrometry analysis, our method was proved to have good sensitivity (1 fmol), high selectivity (1 : 1500, human IgG to bovine serum albumin), high binding capacity (160 mg g-1, IgG/TpPa-1@Ag@GSH), ultra-fast capture ability (only 1 min incubation time), and good reusability (at least 5 times).
30860530	4	74	link	N-linked	1158:1165	arg1	glycopeptides					1167:1179	N-linked glycopeptides	1158:1179	N-linked glycopeptides	1158:1179	Benefiting from the judicious selection of the substrate, the abundance of binding sites, relatively high affinity between GSH and N-linked glycopeptides, and the multivalent interactions between N-linked glycopeptides and unoccupied surfaces of Ag NPs, this porous material showed great performance in N-linked glycopeptide enrichment.
30860530	7	75	theme	popularized	1992:2002	arg1	route					2032:2036	a very facile and easily popularized post-synthetic modification route	1967:2036	a very facile and easily popularized post-synthetic modification route for COFs	1967:2045	Our work improved the enrichment selectivity of COFs, reached the most rapid capture ability among off-column enrichment materials, and provided a very facile and easily popularized post-synthetic modification route for COFs in glycoproteome analysis.
30860530	3	76	theme	nuclear	711:717	arg1	resonance					728:736	nuclear magnetic resonance	711:736	nuclear magnetic resonance spectroscopy	711:749	TpPa-1@Ag@GSH and intermediate products were confirmed and evaluated by nuclear magnetic resonance spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, transmission electron microscopy, scanning electron microscopy-energy dispersive spectroscopy, Brunauer-Emmett-Teller and thermogravimetric analyses.
30860530	5	77	theme	high	1495:1498	arg1	selectivity					1500:1510	high selectivity	1495:1510	high selectivity (1 : 1500, human IgG to bovine serum albumin)	1495:1556	By enriching N-linked glycopeptides in tryptic digests of human serum immunoglobulin G (human IgG) followed by mass spectrometry analysis, our method was proved to have good sensitivity (1 fmol), high selectivity (1 : 1500, human IgG to bovine serum albumin), high binding capacity (160 mg g-1, IgG/TpPa-1@Ag@GSH), ultra-fast capture ability (only 1 min incubation time), and good reusability (at least 5 times).
30860530	1	78	theme	materials	212:220	arg1	essential					283:291	essential	283:291	essential	283:291	The development of facilely synthetic materials for highly efficient enrichment of N-linked glycopeptides is essential in glycoproteome analysis.
30860530	1	78	theme	materials	212:220	arg1	development					178:188	The development	174:188	The development of facilely synthetic materials for highly efficient enrichment of N-linked glycopeptides	174:278	The development of facilely synthetic materials for highly efficient enrichment of N-linked glycopeptides is essential in glycoproteome analysis.
30860530	2	79	theme	step	605:608	arg1	modification					625:636	a simple two step post-synthetic modification	592:636	a simple two step post-synthetic modification	592:636	In this work, by utilizing the self-assembling of glutathione (GSH) on silver nanoparticles (Ag NPs), and the formation and dispersion of Ag NPs on a robust TpPa-1 substrate, a newly functionalized covalent organic framework (COF) called TpPa-1@Ag@GSH was synthesized via a simple two step post-synthetic modification.
30860530	5	80	link	N-linked	1312:1319	arg1	glycopeptides					1321:1333	N-linked glycopeptides	1312:1333	N-linked glycopeptides in tryptic digests of human serum immunoglobulin G (human IgG) followed by mass spectrometry analysis	1312:1435	By enriching N-linked glycopeptides in tryptic digests of human serum immunoglobulin G (human IgG) followed by mass spectrometry analysis, our method was proved to have good sensitivity (1 fmol), high selectivity (1 : 1500, human IgG to bovine serum albumin), high binding capacity (160 mg g-1, IgG/TpPa-1@Ag@GSH), ultra-fast capture ability (only 1 min incubation time), and good reusability (at least 5 times).
30860530	4	81	theme	NPs	1211:1213	arg1	glycopeptides					1167:1179	N-linked glycopeptides	1158:1179	N-linked glycopeptides	1158:1179	Benefiting from the judicious selection of the substrate, the abundance of binding sites, relatively high affinity between GSH and N-linked glycopeptides, and the multivalent interactions between N-linked glycopeptides and unoccupied surfaces of Ag NPs, this porous material showed great performance in N-linked glycopeptide enrichment.
30860530	4	81	theme	NPs	1211:1213	arg1	surfaces					1196:1203	unoccupied surfaces	1185:1203	unoccupied surfaces	1185:1203	Benefiting from the judicious selection of the substrate, the abundance of binding sites, relatively high affinity between GSH and N-linked glycopeptides, and the multivalent interactions between N-linked glycopeptides and unoccupied surfaces of Ag NPs, this porous material showed great performance in N-linked glycopeptide enrichment.
30860530	1	82	gly	glycopeptides	266:278	arg2	glycopeptides					266:278	N-linked glycopeptides	257:278	N-linked glycopeptides	257:278	The development of facilely synthetic materials for highly efficient enrichment of N-linked glycopeptides is essential in glycoproteome analysis.
30860530	2	83	theme	glutathione	370:380	arg1	self-assembling					351:365	self-assembling	351:365	self-assembling	351:365	In this work, by utilizing the self-assembling of glutathione (GSH) on silver nanoparticles (Ag NPs), and the formation and dispersion of Ag NPs on a robust TpPa-1 substrate, a newly functionalized covalent organic framework (COF) called TpPa-1@Ag@GSH was synthesized via a simple two step post-synthetic modification.
30860530	2	84	theme	simple	594:599	arg1	modification					625:636	a simple two step post-synthetic modification	592:636	a simple two step post-synthetic modification	592:636	In this work, by utilizing the self-assembling of glutathione (GSH) on silver nanoparticles (Ag NPs), and the formation and dispersion of Ag NPs on a robust TpPa-1 substrate, a newly functionalized covalent organic framework (COF) called TpPa-1@Ag@GSH was synthesized via a simple two step post-synthetic modification.
30860530	5	85	theme	capture	1625:1631	arg1	time					1664:1667	only 1 min incubation time	1642:1667	only 1 min incubation time	1642:1667	By enriching N-linked glycopeptides in tryptic digests of human serum immunoglobulin G (human IgG) followed by mass spectrometry analysis, our method was proved to have good sensitivity (1 fmol), high selectivity (1 : 1500, human IgG to bovine serum albumin), high binding capacity (160 mg g-1, IgG/TpPa-1@Ag@GSH), ultra-fast capture ability (only 1 min incubation time), and good reusability (at least 5 times).
30860530	5	85	theme	capture	1625:1631	arg1	ability					1633:1639	ultra-fast capture ability	1614:1639	ultra-fast capture ability (only 1 min incubation time)	1614:1668	By enriching N-linked glycopeptides in tryptic digests of human serum immunoglobulin G (human IgG) followed by mass spectrometry analysis, our method was proved to have good sensitivity (1 fmol), high selectivity (1 : 1500, human IgG to bovine serum albumin), high binding capacity (160 mg g-1, IgG/TpPa-1@Ag@GSH), ultra-fast capture ability (only 1 min incubation time), and good reusability (at least 5 times).
30860530	0	86	theme	glycopeptides	159:171	arg1	enrichment					136:145	rapid and highly efficient enrichment	109:145	rapid and highly efficient enrichment of N-linked glycopeptides	109:171	A facilely synthesized glutathione-functionalized silver nanoparticle-grafted covalent organic framework for rapid and highly efficient enrichment of N-linked glycopeptides.
30860530	4	87	link	N-linked	1093:1100	arg1	glycopeptides					1102:1114	N-linked glycopeptides	1093:1114	N-linked glycopeptides	1093:1114	Benefiting from the judicious selection of the substrate, the abundance of binding sites, relatively high affinity between GSH and N-linked glycopeptides, and the multivalent interactions between N-linked glycopeptides and unoccupied surfaces of Ag NPs, this porous material showed great performance in N-linked glycopeptide enrichment.
30860530	2	88	theme	called	551:556	arg1	GSH					568:570	called TpPa-1@Ag@GSH	551:570	called TpPa-1@Ag@GSH	551:570	In this work, by utilizing the self-assembling of glutathione (GSH) on silver nanoparticles (Ag NPs), and the formation and dispersion of Ag NPs on a robust TpPa-1 substrate, a newly functionalized covalent organic framework (COF) called TpPa-1@Ag@GSH was synthesized via a simple two step post-synthetic modification.
30860530	4	89	theme	unoccupied	1185:1194	arg1	surfaces					1196:1203	unoccupied surfaces	1185:1203	unoccupied surfaces	1185:1203	Benefiting from the judicious selection of the substrate, the abundance of binding sites, relatively high affinity between GSH and N-linked glycopeptides, and the multivalent interactions between N-linked glycopeptides and unoccupied surfaces of Ag NPs, this porous material showed great performance in N-linked glycopeptide enrichment.
30860530	5	90	theme	Ag	1605:1606	arg1	GSH					1608:1610	IgG/TpPa-1@Ag@GSH	1594:1610	IgG/TpPa-1@Ag@GSH	1594:1610	By enriching N-linked glycopeptides in tryptic digests of human serum immunoglobulin G (human IgG) followed by mass spectrometry analysis, our method was proved to have good sensitivity (1 fmol), high selectivity (1 : 1500, human IgG to bovine serum albumin), high binding capacity (160 mg g-1, IgG/TpPa-1@Ag@GSH), ultra-fast capture ability (only 1 min incubation time), and good reusability (at least 5 times).
30860530	3	91	theme	resonance	728:736	arg1	spectroscopy					738:749	nuclear magnetic resonance spectroscopy	711:749	nuclear magnetic resonance spectroscopy	711:749	TpPa-1@Ag@GSH and intermediate products were confirmed and evaluated by nuclear magnetic resonance spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, transmission electron microscopy, scanning electron microscopy-energy dispersive spectroscopy, Brunauer-Emmett-Teller and thermogravimetric analyses.
30860530	1	92	from	analysis	310:317	arg1	essential					283:291	essential	283:291	essential	283:291	The development of facilely synthetic materials for highly efficient enrichment of N-linked glycopeptides is essential in glycoproteome analysis.
30860530	1	92	from	analysis	310:317	arg1	development					178:188	The development	174:188	The development of facilely synthetic materials for highly efficient enrichment of N-linked glycopeptides	174:278	The development of facilely synthetic materials for highly efficient enrichment of N-linked glycopeptides is essential in glycoproteome analysis.
30860530	1	93	theme	N-linked	257:264	arg1	glycopeptides					266:278	N-linked glycopeptides	257:278	N-linked glycopeptides	257:278	The development of facilely synthetic materials for highly efficient enrichment of N-linked glycopeptides is essential in glycoproteome analysis.
30860530	7	94	theme	enrichment	1932:1941	arg1	materials					1943:1951	off-column enrichment materials	1921:1951	off-column enrichment materials	1921:1951	Our work improved the enrichment selectivity of COFs, reached the most rapid capture ability among off-column enrichment materials, and provided a very facile and easily popularized post-synthetic modification route for COFs in glycoproteome analysis.
30860530	4	95	theme	judicious	982:990	arg1	selection					992:1000	the judicious selection	978:1000	the judicious selection of the substrate	978:1017	Benefiting from the judicious selection of the substrate, the abundance of binding sites, relatively high affinity between GSH and N-linked glycopeptides, and the multivalent interactions between N-linked glycopeptides and unoccupied surfaces of Ag NPs, this porous material showed great performance in N-linked glycopeptide enrichment.
30860530	4	96	theme	great	1244:1248	arg1	performance					1250:1260	great performance	1244:1260	great performance in N-linked glycopeptide enrichment	1244:1296	Benefiting from the judicious selection of the substrate, the abundance of binding sites, relatively high affinity between GSH and N-linked glycopeptides, and the multivalent interactions between N-linked glycopeptides and unoccupied surfaces of Ag NPs, this porous material showed great performance in N-linked glycopeptide enrichment.
30860530	2	97	theme	@	567:567	arg1	GSH					568:570	called TpPa-1@Ag@GSH	551:570	called TpPa-1@Ag@GSH	551:570	In this work, by utilizing the self-assembling of glutathione (GSH) on silver nanoparticles (Ag NPs), and the formation and dispersion of Ag NPs on a robust TpPa-1 substrate, a newly functionalized covalent organic framework (COF) called TpPa-1@Ag@GSH was synthesized via a simple two step post-synthetic modification.
30860530	5	98	dep	time	1664:1667	arg1	min					1649:1651	min	1649:1651	min	1649:1651	By enriching N-linked glycopeptides in tryptic digests of human serum immunoglobulin G (human IgG) followed by mass spectrometry analysis, our method was proved to have good sensitivity (1 fmol), high selectivity (1 : 1500, human IgG to bovine serum albumin), high binding capacity (160 mg g-1, IgG/TpPa-1@Ag@GSH), ultra-fast capture ability (only 1 min incubation time), and good reusability (at least 5 times).
30860530	5	99	theme	human	1357:1361	arg1	IgG					1393:1395	human IgG	1387:1395	human IgG	1387:1395	By enriching N-linked glycopeptides in tryptic digests of human serum immunoglobulin G (human IgG) followed by mass spectrometry analysis, our method was proved to have good sensitivity (1 fmol), high selectivity (1 : 1500, human IgG to bovine serum albumin), high binding capacity (160 mg g-1, IgG/TpPa-1@Ag@GSH), ultra-fast capture ability (only 1 min incubation time), and good reusability (at least 5 times).
30860530	5	99	theme	human	1357:1361	arg1	G					1384:1384	human serum immunoglobulin G	1357:1384	human serum immunoglobulin G (human IgG)	1357:1396	By enriching N-linked glycopeptides in tryptic digests of human serum immunoglobulin G (human IgG) followed by mass spectrometry analysis, our method was proved to have good sensitivity (1 fmol), high selectivity (1 : 1500, human IgG to bovine serum albumin), high binding capacity (160 mg g-1, IgG/TpPa-1@Ag@GSH), ultra-fast capture ability (only 1 min incubation time), and good reusability (at least 5 times).
30860530	4	100	gly	glycopeptides	1102:1114	arg2	glycopeptides					1102:1114	N-linked glycopeptides	1093:1114	N-linked glycopeptides	1093:1114	Benefiting from the judicious selection of the substrate, the abundance of binding sites, relatively high affinity between GSH and N-linked glycopeptides, and the multivalent interactions between N-linked glycopeptides and unoccupied surfaces of Ag NPs, this porous material showed great performance in N-linked glycopeptide enrichment.
30860530	3	101	theme	dispersive	882:891	arg1	spectroscopy					893:904	scanning electron microscopy-energy dispersive spectroscopy	846:904	scanning electron microscopy-energy dispersive spectroscopy	846:904	TpPa-1@Ag@GSH and intermediate products were confirmed and evaluated by nuclear magnetic resonance spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, transmission electron microscopy, scanning electron microscopy-energy dispersive spectroscopy, Brunauer-Emmett-Teller and thermogravimetric analyses.
30860530	2	102	theme	@	564:564	arg1	GSH					568:570	called TpPa-1@Ag@GSH	551:570	called TpPa-1@Ag@GSH	551:570	In this work, by utilizing the self-assembling of glutathione (GSH) on silver nanoparticles (Ag NPs), and the formation and dispersion of Ag NPs on a robust TpPa-1 substrate, a newly functionalized covalent organic framework (COF) called TpPa-1@Ag@GSH was synthesized via a simple two step post-synthetic modification.
30860530	5	103	dep	min	1649:1651	arg1	1					1647:1647	1	1647:1647	1	1647:1647	By enriching N-linked glycopeptides in tryptic digests of human serum immunoglobulin G (human IgG) followed by mass spectrometry analysis, our method was proved to have good sensitivity (1 fmol), high selectivity (1 : 1500, human IgG to bovine serum albumin), high binding capacity (160 mg g-1, IgG/TpPa-1@Ag@GSH), ultra-fast capture ability (only 1 min incubation time), and good reusability (at least 5 times).
30860530	5	104	theme	immunoglobulin	1369:1382	arg1	IgG					1393:1395	human IgG	1387:1395	human IgG	1387:1395	By enriching N-linked glycopeptides in tryptic digests of human serum immunoglobulin G (human IgG) followed by mass spectrometry analysis, our method was proved to have good sensitivity (1 fmol), high selectivity (1 : 1500, human IgG to bovine serum albumin), high binding capacity (160 mg g-1, IgG/TpPa-1@Ag@GSH), ultra-fast capture ability (only 1 min incubation time), and good reusability (at least 5 times).
30860530	5	104	theme	immunoglobulin	1369:1382	arg1	G					1384:1384	human serum immunoglobulin G	1357:1384	human serum immunoglobulin G (human IgG)	1357:1396	By enriching N-linked glycopeptides in tryptic digests of human serum immunoglobulin G (human IgG) followed by mass spectrometry analysis, our method was proved to have good sensitivity (1 fmol), high selectivity (1 : 1500, human IgG to bovine serum albumin), high binding capacity (160 mg g-1, IgG/TpPa-1@Ag@GSH), ultra-fast capture ability (only 1 min incubation time), and good reusability (at least 5 times).
30860530	3	105	theme	electron	855:862	arg1	spectroscopy					893:904	scanning electron microscopy-energy dispersive spectroscopy	846:904	scanning electron microscopy-energy dispersive spectroscopy	846:904	TpPa-1@Ag@GSH and intermediate products were confirmed and evaluated by nuclear magnetic resonance spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, transmission electron microscopy, scanning electron microscopy-energy dispersive spectroscopy, Brunauer-Emmett-Teller and thermogravimetric analyses.
30860530	6	106	gly	glycopeptides	1775:1787	arg1	samples					1813:1819	complex biological samples	1794:1819	complex biological samples	1794:1819	It was also successfully applied to the enrichment of N-linked glycopeptides from complex biological samples.
30860530	6	106	gly	glycopeptides	1775:1787	arg2	glycopeptides					1775:1787	N-linked glycopeptides	1766:1787	N-linked glycopeptides from complex biological samples	1766:1819	It was also successfully applied to the enrichment of N-linked glycopeptides from complex biological samples.
30860530	3	107	theme	X-ray	793:797	arg1	diffraction					799:809	X-ray diffraction	793:809	X-ray diffraction	793:809	TpPa-1@Ag@GSH and intermediate products were confirmed and evaluated by nuclear magnetic resonance spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, transmission electron microscopy, scanning electron microscopy-energy dispersive spectroscopy, Brunauer-Emmett-Teller and thermogravimetric analyses.
30860530	7	108	theme	rapid	1893:1897	arg1	ability					1907:1913	the most rapid capture ability	1884:1913	the most rapid capture ability	1884:1913	Our work improved the enrichment selectivity of COFs, reached the most rapid capture ability among off-column enrichment materials, and provided a very facile and easily popularized post-synthetic modification route for COFs in glycoproteome analysis.
30860530	1	109	theme	facilely	193:200	arg1	materials					212:220	facilely synthetic materials	193:220	facilely synthetic materials for highly efficient enrichment of N-linked glycopeptides	193:278	The development of facilely synthetic materials for highly efficient enrichment of N-linked glycopeptides is essential in glycoproteome analysis.
30860530	0	110	theme	efficient	126:134	arg1	enrichment					136:145	rapid and highly efficient enrichment	109:145	rapid and highly efficient enrichment of N-linked glycopeptides	109:171	A facilely synthesized glutathione-functionalized silver nanoparticle-grafted covalent organic framework for rapid and highly efficient enrichment of N-linked glycopeptides.
30860530	5	111	gly	glycopeptides	1321:1333	arg2	glycopeptides					1321:1333	N-linked glycopeptides	1312:1333	N-linked glycopeptides in tryptic digests of human serum immunoglobulin G (human IgG) followed by mass spectrometry analysis	1312:1435	By enriching N-linked glycopeptides in tryptic digests of human serum immunoglobulin G (human IgG) followed by mass spectrometry analysis, our method was proved to have good sensitivity (1 fmol), high selectivity (1 : 1500, human IgG to bovine serum albumin), high binding capacity (160 mg g-1, IgG/TpPa-1@Ag@GSH), ultra-fast capture ability (only 1 min incubation time), and good reusability (at least 5 times).
30860530	3	112	theme	electron	825:832	arg1	microscopy					834:843	transmission electron microscopy	812:843	transmission electron microscopy	812:843	TpPa-1@Ag@GSH and intermediate products were confirmed and evaluated by nuclear magnetic resonance spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, transmission electron microscopy, scanning electron microscopy-energy dispersive spectroscopy, Brunauer-Emmett-Teller and thermogravimetric analyses.
30860530	4	113	gly	glycopeptides	1167:1179	arg2	glycopeptides					1167:1179	N-linked glycopeptides	1158:1179	N-linked glycopeptides	1158:1179	Benefiting from the judicious selection of the substrate, the abundance of binding sites, relatively high affinity between GSH and N-linked glycopeptides, and the multivalent interactions between N-linked glycopeptides and unoccupied surfaces of Ag NPs, this porous material showed great performance in N-linked glycopeptide enrichment.
30860530	4	113	gly	glycopeptides	1167:1179	arg1	NPs					1211:1213	Ag NPs	1208:1213	Ag NPs	1208:1213	Benefiting from the judicious selection of the substrate, the abundance of binding sites, relatively high affinity between GSH and N-linked glycopeptides, and the multivalent interactions between N-linked glycopeptides and unoccupied surfaces of Ag NPs, this porous material showed great performance in N-linked glycopeptide enrichment.
30860530	2	114	theme	covalent	518:525	arg1	COF					546:548	COF	546:548	COF	546:548	In this work, by utilizing the self-assembling of glutathione (GSH) on silver nanoparticles (Ag NPs), and the formation and dispersion of Ag NPs on a robust TpPa-1 substrate, a newly functionalized covalent organic framework (COF) called TpPa-1@Ag@GSH was synthesized via a simple two step post-synthetic modification.
30860530	2	114	theme	covalent	518:525	arg1	framework					535:543	a newly functionalized covalent organic framework	495:543	a newly functionalized covalent organic framework (COF)	495:549	In this work, by utilizing the self-assembling of glutathione (GSH) on silver nanoparticles (Ag NPs), and the formation and dispersion of Ag NPs on a robust TpPa-1 substrate, a newly functionalized covalent organic framework (COF) called TpPa-1@Ag@GSH was synthesized via a simple two step post-synthetic modification.
30860530	2	114	theme	covalent	518:525	arg1	substrate					484:492	a robust TpPa-1 substrate	468:492	a robust TpPa-1 substrate	468:492	In this work, by utilizing the self-assembling of glutathione (GSH) on silver nanoparticles (Ag NPs), and the formation and dispersion of Ag NPs on a robust TpPa-1 substrate, a newly functionalized covalent organic framework (COF) called TpPa-1@Ag@GSH was synthesized via a simple two step post-synthetic modification.
30860530	7	115	theme	off-column	1921:1930	arg1	materials					1943:1951	off-column enrichment materials	1921:1951	off-column enrichment materials	1921:1951	Our work improved the enrichment selectivity of COFs, reached the most rapid capture ability among off-column enrichment materials, and provided a very facile and easily popularized post-synthetic modification route for COFs in glycoproteome analysis.
30860530	4	116	theme	glycopeptide	1274:1285	arg1	enrichment					1287:1296	N-linked glycopeptide enrichment	1265:1296	N-linked glycopeptide enrichment	1265:1296	Benefiting from the judicious selection of the substrate, the abundance of binding sites, relatively high affinity between GSH and N-linked glycopeptides, and the multivalent interactions between N-linked glycopeptides and unoccupied surfaces of Ag NPs, this porous material showed great performance in N-linked glycopeptide enrichment.
30860530	5	117	theme	mass	1410:1413	arg1	analysis					1428:1435	mass spectrometry analysis	1410:1435	mass spectrometry analysis	1410:1435	By enriching N-linked glycopeptides in tryptic digests of human serum immunoglobulin G (human IgG) followed by mass spectrometry analysis, our method was proved to have good sensitivity (1 fmol), high selectivity (1 : 1500, human IgG to bovine serum albumin), high binding capacity (160 mg g-1, IgG/TpPa-1@Ag@GSH), ultra-fast capture ability (only 1 min incubation time), and good reusability (at least 5 times).
30860530	0	118	link	N-linked	150:157	arg1	glycopeptides					159:171	N-linked glycopeptides	150:171	N-linked glycopeptides	150:171	A facilely synthesized glutathione-functionalized silver nanoparticle-grafted covalent organic framework for rapid and highly efficient enrichment of N-linked glycopeptides.
30860530	2	119	theme	NPs	461:463	arg1	formation					430:438	the formation	426:438	the formation	426:438	In this work, by utilizing the self-assembling of glutathione (GSH) on silver nanoparticles (Ag NPs), and the formation and dispersion of Ag NPs on a robust TpPa-1 substrate, a newly functionalized covalent organic framework (COF) called TpPa-1@Ag@GSH was synthesized via a simple two step post-synthetic modification.
30860530	2	119	theme	NPs	461:463	arg1	dispersion					444:453	dispersion	444:453	dispersion of Ag NPs on a robust TpPa-1 substrate, a newly functionalized covalent organic framework (COF)	444:549	In this work, by utilizing the self-assembling of glutathione (GSH) on silver nanoparticles (Ag NPs), and the formation and dispersion of Ag NPs on a robust TpPa-1 substrate, a newly functionalized covalent organic framework (COF) called TpPa-1@Ag@GSH was synthesized via a simple two step post-synthetic modification.
30860530	0	120	theme	glutathione-functionalized	23:48	arg1	silver					50:55	A facilely synthesized glutathione-functionalized silver	0:55	A facilely synthesized glutathione-functionalized silver	0:55	A facilely synthesized glutathione-functionalized silver nanoparticle-grafted covalent organic framework for rapid and highly efficient enrichment of N-linked glycopeptides.
30860530	7	121	theme	enrichment	1844:1853	arg1	selectivity					1855:1865	the enrichment selectivity	1840:1865	the enrichment selectivity of COFs	1840:1873	Our work improved the enrichment selectivity of COFs, reached the most rapid capture ability among off-column enrichment materials, and provided a very facile and easily popularized post-synthetic modification route for COFs in glycoproteome analysis.
30860530	6	122	link	N-linked	1766:1773	arg1	glycopeptides					1775:1787	N-linked glycopeptides	1766:1787	N-linked glycopeptides from complex biological samples	1766:1819	It was also successfully applied to the enrichment of N-linked glycopeptides from complex biological samples.
30860530	3	123	theme	TpPa-1	639:644	arg1	GSH					649:651	TpPa-1@Ag@GSH and intermediate products	639:677	GSH	649:651	TpPa-1@Ag@GSH and intermediate products were confirmed and evaluated by nuclear magnetic resonance spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, transmission electron microscopy, scanning electron microscopy-energy dispersive spectroscopy, Brunauer-Emmett-Teller and thermogravimetric analyses.
30860530	5	124	dep	g-1	1589:1591	arg1	GSH					1608:1610	IgG/TpPa-1@Ag@GSH	1594:1610	IgG/TpPa-1@Ag@GSH	1594:1610	By enriching N-linked glycopeptides in tryptic digests of human serum immunoglobulin G (human IgG) followed by mass spectrometry analysis, our method was proved to have good sensitivity (1 fmol), high selectivity (1 : 1500, human IgG to bovine serum albumin), high binding capacity (160 mg g-1, IgG/TpPa-1@Ag@GSH), ultra-fast capture ability (only 1 min incubation time), and good reusability (at least 5 times).
30860530	2	125	theme	TpPa-1	477:482	arg1	framework					535:543	a newly functionalized covalent organic framework	495:543	a newly functionalized covalent organic framework (COF)	495:549	In this work, by utilizing the self-assembling of glutathione (GSH) on silver nanoparticles (Ag NPs), and the formation and dispersion of Ag NPs on a robust TpPa-1 substrate, a newly functionalized covalent organic framework (COF) called TpPa-1@Ag@GSH was synthesized via a simple two step post-synthetic modification.
30860530	2	125	theme	TpPa-1	477:482	arg1	substrate					484:492	a robust TpPa-1 substrate	468:492	a robust TpPa-1 substrate	468:492	In this work, by utilizing the self-assembling of glutathione (GSH) on silver nanoparticles (Ag NPs), and the formation and dispersion of Ag NPs on a robust TpPa-1 substrate, a newly functionalized covalent organic framework (COF) called TpPa-1@Ag@GSH was synthesized via a simple two step post-synthetic modification.
30860530	5	126	theme	binding	1564:1570	arg1	capacity					1572:1579	high binding capacity	1559:1579	high binding capacity (160 mg g-1, IgG/TpPa-1@Ag@GSH)	1559:1611	By enriching N-linked glycopeptides in tryptic digests of human serum immunoglobulin G (human IgG) followed by mass spectrometry analysis, our method was proved to have good sensitivity (1 fmol), high selectivity (1 : 1500, human IgG to bovine serum albumin), high binding capacity (160 mg g-1, IgG/TpPa-1@Ag@GSH), ultra-fast capture ability (only 1 min incubation time), and good reusability (at least 5 times).
30860530	5	126	theme	binding	1564:1570	arg1	g-1					1589:1591	160 mg g-1	1582:1591	160 mg g-1	1582:1591	By enriching N-linked glycopeptides in tryptic digests of human serum immunoglobulin G (human IgG) followed by mass spectrometry analysis, our method was proved to have good sensitivity (1 fmol), high selectivity (1 : 1500, human IgG to bovine serum albumin), high binding capacity (160 mg g-1, IgG/TpPa-1@Ag@GSH), ultra-fast capture ability (only 1 min incubation time), and good reusability (at least 5 times).
30860530	4	127	theme	substrate	1009:1017	arg1	abundance					1024:1032	the abundance	1020:1032	the abundance of binding sites	1020:1049	Benefiting from the judicious selection of the substrate, the abundance of binding sites, relatively high affinity between GSH and N-linked glycopeptides, and the multivalent interactions between N-linked glycopeptides and unoccupied surfaces of Ag NPs, this porous material showed great performance in N-linked glycopeptide enrichment.
30860530	4	127	theme	substrate	1009:1017	arg1	interactions					1137:1148	the multivalent interactions	1121:1148	the multivalent interactions between N-linked glycopeptides and unoccupied surfaces of Ag NPs	1121:1213	Benefiting from the judicious selection of the substrate, the abundance of binding sites, relatively high affinity between GSH and N-linked glycopeptides, and the multivalent interactions between N-linked glycopeptides and unoccupied surfaces of Ag NPs, this porous material showed great performance in N-linked glycopeptide enrichment.
30860530	4	127	theme	substrate	1009:1017	arg1	affinity					1068:1075	relatively high affinity	1052:1075	relatively high affinity between GSH and N-linked glycopeptides	1052:1114	Benefiting from the judicious selection of the substrate, the abundance of binding sites, relatively high affinity between GSH and N-linked glycopeptides, and the multivalent interactions between N-linked glycopeptides and unoccupied surfaces of Ag NPs, this porous material showed great performance in N-linked glycopeptide enrichment.
30860530	4	127	theme	substrate	1009:1017	arg1	selection					992:1000	the judicious selection	978:1000	the judicious selection of the substrate	978:1017	Benefiting from the judicious selection of the substrate, the abundance of binding sites, relatively high affinity between GSH and N-linked glycopeptides, and the multivalent interactions between N-linked glycopeptides and unoccupied surfaces of Ag NPs, this porous material showed great performance in N-linked glycopeptide enrichment.
30860530	0	128	theme	organic	87:93	arg1	framework					95:103	covalent organic framework	78:103	covalent organic framework for rapid and highly efficient enrichment of N-linked glycopeptides	78:171	A facilely synthesized glutathione-functionalized silver nanoparticle-grafted covalent organic framework for rapid and highly efficient enrichment of N-linked glycopeptides.
30860530	7	129	theme	modification	2019:2030	arg1	route					2032:2036	a very facile and easily popularized post-synthetic modification route	1967:2036	a very facile and easily popularized post-synthetic modification route for COFs	1967:2045	Our work improved the enrichment selectivity of COFs, reached the most rapid capture ability among off-column enrichment materials, and provided a very facile and easily popularized post-synthetic modification route for COFs in glycoproteome analysis.
30860530	5	130	theme	bovine	1536:1541	arg1	albumin					1549:1555	bovine serum albumin	1536:1555	bovine serum albumin	1536:1555	By enriching N-linked glycopeptides in tryptic digests of human serum immunoglobulin G (human IgG) followed by mass spectrometry analysis, our method was proved to have good sensitivity (1 fmol), high selectivity (1 : 1500, human IgG to bovine serum albumin), high binding capacity (160 mg g-1, IgG/TpPa-1@Ag@GSH), ultra-fast capture ability (only 1 min incubation time), and good reusability (at least 5 times).
30860530	6	131	theme	biological	1802:1811	arg1	samples					1813:1819	complex biological samples	1794:1819	complex biological samples	1794:1819	It was also successfully applied to the enrichment of N-linked glycopeptides from complex biological samples.
30860530	3	132	theme	Ag	646:647	arg1	GSH					649:651	TpPa-1@Ag@GSH and intermediate products	639:677	GSH	649:651	TpPa-1@Ag@GSH and intermediate products were confirmed and evaluated by nuclear magnetic resonance spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, transmission electron microscopy, scanning electron microscopy-energy dispersive spectroscopy, Brunauer-Emmett-Teller and thermogravimetric analyses.
30860530	7	133	theme	post-synthetic	2004:2017	arg1	route					2032:2036	a very facile and easily popularized post-synthetic modification route	1967:2036	a very facile and easily popularized post-synthetic modification route for COFs	1967:2045	Our work improved the enrichment selectivity of COFs, reached the most rapid capture ability among off-column enrichment materials, and provided a very facile and easily popularized post-synthetic modification route for COFs in glycoproteome analysis.
30860530	5	134	theme	good	1675:1678	arg1	reusability					1680:1690	good reusability	1675:1690	good reusability (at least 5 times)	1675:1709	By enriching N-linked glycopeptides in tryptic digests of human serum immunoglobulin G (human IgG) followed by mass spectrometry analysis, our method was proved to have good sensitivity (1 fmol), high selectivity (1 : 1500, human IgG to bovine serum albumin), high binding capacity (160 mg g-1, IgG/TpPa-1@Ag@GSH), ultra-fast capture ability (only 1 min incubation time), and good reusability (at least 5 times).
30860530	5	134	theme	good	1675:1678	arg1	times					1704:1708	at least 5 times	1693:1708	at least 5 times	1693:1708	By enriching N-linked glycopeptides in tryptic digests of human serum immunoglobulin G (human IgG) followed by mass spectrometry analysis, our method was proved to have good sensitivity (1 fmol), high selectivity (1 : 1500, human IgG to bovine serum albumin), high binding capacity (160 mg g-1, IgG/TpPa-1@Ag@GSH), ultra-fast capture ability (only 1 min incubation time), and good reusability (at least 5 times).
30860530	1	135	theme	glycoproteome	296:308	arg1	analysis					310:317	glycoproteome analysis	296:317	glycoproteome analysis	296:317	The development of facilely synthetic materials for highly efficient enrichment of N-linked glycopeptides is essential in glycoproteome analysis.
29775232	0	0	theme	yeast	100:104	arg1	cerevisiae					120:129	the yeast Saccharomyces cerevisiae	96:129	the yeast Saccharomyces cerevisiae	96:129	Mannosylinositol phosphorylceramides and ergosterol coodinately maintain cell wall integrity in the yeast Saccharomyces cerevisiae.
29775232	4	1	from	defect	840:845	arg1	CWI					880:882	CWI	880:882	CWI	880:882	In addition, repression of expression of ERG9, which encodes squalene synthase in the ergosterol biosynthesis pathway, in sur1∆ csh1∆ cells caused a strong growth defect and enhancement of the defect in CWI.
29775232	4	2	theme	growth	833:838	arg1	defect					840:845	a strong growth defect	824:845	a strong growth defect	824:845	In addition, repression of expression of ERG9, which encodes squalene synthase in the ergosterol biosynthesis pathway, in sur1∆ csh1∆ cells caused a strong growth defect and enhancement of the defect in CWI.
29775232	3	3	dep	synthases	559:567	arg1	Csh1					579:582	Csh1	579:582	Csh1	579:582	In this study, we screened for multicopy suppressor genes that rescue the defect in CWI in cells lacking MIPC synthases (Sur1 and Csh1), and found that the defect is partly suppressed by upregulation of ergosterol biosynthesis.
29775232	3	3	dep	synthases	559:567	arg1	Sur1					570:573	Sur1	570:573	Sur1	570:573	In this study, we screened for multicopy suppressor genes that rescue the defect in CWI in cells lacking MIPC synthases (Sur1 and Csh1), and found that the defect is partly suppressed by upregulation of ergosterol biosynthesis.
29775232	3	3	dep	synthases	559:567	arg1	synthases					559:567	MIPC synthases	554:567	MIPC synthases (Sur1 and Csh1)	554:583	In this study, we screened for multicopy suppressor genes that rescue the defect in CWI in cells lacking MIPC synthases (Sur1 and Csh1), and found that the defect is partly suppressed by upregulation of ergosterol biosynthesis.
29775232	1	4	theme	composition	261:271	arg1	breakdown					235:243	breakdown	235:243	breakdown of their normal composition	235:271	In the yeast Saccharomyces cerevisiae, complex sphingolipids have three types of polar head group, and breakdown of their normal composition causes several cellular dysfunctions.
29775232	3	5	theme	ergosterol	652:661	arg1	biosynthesis					663:674	ergosterol biosynthesis	652:674	ergosterol biosynthesis	652:674	In this study, we screened for multicopy suppressor genes that rescue the defect in CWI in cells lacking MIPC synthases (Sur1 and Csh1), and found that the defect is partly suppressed by upregulation of ergosterol biosynthesis.
29775232	4	6	theme	strong	826:831	arg1	defect					840:845	a strong growth defect	824:845	a strong growth defect	824:845	In addition, repression of expression of ERG9, which encodes squalene synthase in the ergosterol biosynthesis pathway, in sur1∆ csh1∆ cells caused a strong growth defect and enhancement of the defect in CWI.
29775232	4	7	from	synthase	747:754	arg1	pathway					787:793	the ergosterol biosynthesis pathway	759:793	the ergosterol biosynthesis pathway	759:793	In addition, repression of expression of ERG9, which encodes squalene synthase in the ergosterol biosynthesis pathway, in sur1∆ csh1∆ cells caused a strong growth defect and enhancement of the defect in CWI.
29775232	3	8	theme	biosynthesis	663:674	arg1	upregulation					636:647	upregulation	636:647	upregulation of ergosterol biosynthesis	636:674	In this study, we screened for multicopy suppressor genes that rescue the defect in CWI in cells lacking MIPC synthases (Sur1 and Csh1), and found that the defect is partly suppressed by upregulation of ergosterol biosynthesis.
29775232	6	9	theme	sur1∆	1206:1210	arg1	cells					1218:1222	the ERG9-repressed sur1∆ csh1∆ cells	1187:1222	the ERG9-repressed sur1∆ csh1∆ cells	1187:1222	Moreover, the deletion of SLT2 or WSC1/2, encoding a sensor protein recognizing CWI, enhanced the growth defect in the ERG9-repressed sur1∆ csh1∆ cells.
29775232	6	10	from	defect	1177:1182	arg1	cells					1218:1222	the ERG9-repressed sur1∆ csh1∆ cells	1187:1222	the ERG9-repressed sur1∆ csh1∆ cells	1187:1222	Moreover, the deletion of SLT2 or WSC1/2, encoding a sensor protein recognizing CWI, enhanced the growth defect in the ERG9-repressed sur1∆ csh1∆ cells.
29775232	4	11	theme	ERG9	718:721	arg1	expression					704:713	expression	704:713	expression of ERG9, which encodes squalene synthase in the ergosterol biosynthesis pathway, in sur1∆ csh1∆ cells	704:815	In addition, repression of expression of ERG9, which encodes squalene synthase in the ergosterol biosynthesis pathway, in sur1∆ csh1∆ cells caused a strong growth defect and enhancement of the defect in CWI.
29775232	1	12	theme	several	280:286	arg1	dysfunctions					297:308	several cellular dysfunctions	280:308	several cellular dysfunctions	280:308	In the yeast Saccharomyces cerevisiae, complex sphingolipids have three types of polar head group, and breakdown of their normal composition causes several cellular dysfunctions.
29775232	0	13	theme	Saccharomyces	106:118	arg1	cerevisiae					120:129	the yeast Saccharomyces cerevisiae	96:129	the yeast Saccharomyces cerevisiae	96:129	Mannosylinositol phosphorylceramides and ergosterol coodinately maintain cell wall integrity in the yeast Saccharomyces cerevisiae.
29775232	1	14	contain	have	193:196	arg1	sphingolipids					179:191	complex sphingolipids	171:191	complex sphingolipids	171:191	In the yeast Saccharomyces cerevisiae, complex sphingolipids have three types of polar head group, and breakdown of their normal composition causes several cellular dysfunctions.
29775232	1	14	contain	have	193:196	arg2	types					204:208	three types	198:208	three types of polar head group	198:228	In the yeast Saccharomyces cerevisiae, complex sphingolipids have three types of polar head group, and breakdown of their normal composition causes several cellular dysfunctions.
29775232	6	15	theme	ERG9-repressed	1191:1204	arg1	cells					1218:1222	the ERG9-repressed sur1∆ csh1∆ cells	1187:1222	the ERG9-repressed sur1∆ csh1∆ cells	1187:1222	Moreover, the deletion of SLT2 or WSC1/2, encoding a sensor protein recognizing CWI, enhanced the growth defect in the ERG9-repressed sur1∆ csh1∆ cells.
29775232	7	16	theme	Slt2-	1343:1347	arg1	manner					1372:1377	a Slt2- and Wsc1/2-independent manner	1341:1377	a Slt2- and Wsc1/2-independent manner	1341:1377	On the other hand, the ERG9-repressed sur1∆ csh1∆ cells also exhibited an increase in the cell wall chitin level in a Slt2- and Wsc1/2-independent manner.
29775232	1	17	theme	cellular	288:295	arg1	dysfunctions					297:308	several cellular dysfunctions	280:308	several cellular dysfunctions	280:308	In the yeast Saccharomyces cerevisiae, complex sphingolipids have three types of polar head group, and breakdown of their normal composition causes several cellular dysfunctions.
29775232	3	18	theme	suppressor	490:499	arg1	genes					501:505	multicopy suppressor genes	480:505	multicopy suppressor genes that rescue the defect in CWI in cells lacking MIPC synthases (Sur1 and Csh1)	480:583	In this study, we screened for multicopy suppressor genes that rescue the defect in CWI in cells lacking MIPC synthases (Sur1 and Csh1), and found that the defect is partly suppressed by upregulation of ergosterol biosynthesis.
29775232	3	19	from	defect	523:528	arg1	cells					540:544	cells	540:544	cells lacking MIPC synthases (Sur1 and Csh1)	540:583	In this study, we screened for multicopy suppressor genes that rescue the defect in CWI in cells lacking MIPC synthases (Sur1 and Csh1), and found that the defect is partly suppressed by upregulation of ergosterol biosynthesis.
29775232	3	19	from	defect	523:528	arg1	CWI					533:535	CWI	533:535	CWI	533:535	In this study, we screened for multicopy suppressor genes that rescue the defect in CWI in cells lacking MIPC synthases (Sur1 and Csh1), and found that the defect is partly suppressed by upregulation of ergosterol biosynthesis.
29775232	6	20	theme	WSC1/2	1106:1111	arg1	deletion					1086:1093	the deletion	1082:1093	the deletion of SLT2 or WSC1/2, encoding a sensor protein recognizing CWI,	1082:1155	Moreover, the deletion of SLT2 or WSC1/2, encoding a sensor protein recognizing CWI, enhanced the growth defect in the ERG9-repressed sur1∆ csh1∆ cells.
29775232	7	21	theme	sur1∆	1263:1267	arg1	cells					1275:1279	the ERG9-repressed sur1∆ csh1∆ cells	1244:1279	the ERG9-repressed sur1∆ csh1∆ cells	1244:1279	On the other hand, the ERG9-repressed sur1∆ csh1∆ cells also exhibited an increase in the cell wall chitin level in a Slt2- and Wsc1/2-independent manner.
29775232	2	22	theme	cell	422:425	arg1	integrity					432:440	cell wall integrity	422:440	cell wall integrity (CWI)	422:446	Previously we found that loss of biosynthesis of mannosylinositol phosphorylceramide (MIPC) causes a defect in cell wall integrity (CWI).
29775232	2	22	theme	cell	422:425	arg1	CWI					443:445	CWI	443:445	CWI	443:445	Previously we found that loss of biosynthesis of mannosylinositol phosphorylceramide (MIPC) causes a defect in cell wall integrity (CWI).
29775232	4	23	from	cells	811:815	arg1	repression					690:699	repression	690:699	repression of expression of ERG9, which encodes squalene synthase in the ergosterol biosynthesis pathway, in sur1∆ csh1∆ cells	690:815	In addition, repression of expression of ERG9, which encodes squalene synthase in the ergosterol biosynthesis pathway, in sur1∆ csh1∆ cells caused a strong growth defect and enhancement of the defect in CWI.
29775232	4	24	theme	csh1∆	805:809	arg1	cells					811:815	sur1∆ csh1∆ cells	799:815	sur1∆ csh1∆ cells	799:815	In addition, repression of expression of ERG9, which encodes squalene synthase in the ergosterol biosynthesis pathway, in sur1∆ csh1∆ cells caused a strong growth defect and enhancement of the defect in CWI.
29775232	7	25	theme	other	1232:1236	arg1	hand					1238:1241	the other hand	1228:1241	the other hand	1228:1241	On the other hand, the ERG9-repressed sur1∆ csh1∆ cells also exhibited an increase in the cell wall chitin level in a Slt2- and Wsc1/2-independent manner.
29775232	4	26	theme	squalene	738:745	arg1	ERG9					718:721	ERG9	718:721	ERG9	718:721	In addition, repression of expression of ERG9, which encodes squalene synthase in the ergosterol biosynthesis pathway, in sur1∆ csh1∆ cells caused a strong growth defect and enhancement of the defect in CWI.
29775232	4	26	theme	squalene	738:745	arg1	synthase					747:754	squalene synthase	738:754	squalene synthase in the ergosterol biosynthesis pathway	738:793	In addition, repression of expression of ERG9, which encodes squalene synthase in the ergosterol biosynthesis pathway, in sur1∆ csh1∆ cells caused a strong growth defect and enhancement of the defect in CWI.
29775232	7	27	theme	wall	1320:1323	arg1	level					1332:1336	the cell wall chitin level	1311:1336	the cell wall chitin level	1311:1336	On the other hand, the ERG9-repressed sur1∆ csh1∆ cells also exhibited an increase in the cell wall chitin level in a Slt2- and Wsc1/2-independent manner.
29775232	5	28	theme	protein	1020:1026	arg1	Slt2					994:997	Slt2	994:997	Slt2	994:997	The repression of ERG9 and/or the deletion of SUR1 and CSH1 caused an increase in the phosphorylated form of Slt2, a mitogen-activated protein kinase activated through impairment of CWI.
29775232	5	28	theme	protein	1020:1026	arg1	kinase					1028:1033	a mitogen-activated protein kinase	1000:1033	a mitogen-activated protein kinase activated through impairment of CWI	1000:1069	The repression of ERG9 and/or the deletion of SUR1 and CSH1 caused an increase in the phosphorylated form of Slt2, a mitogen-activated protein kinase activated through impairment of CWI.
29775232	7	29	theme	Wsc1/2-independent	1353:1370	arg1	manner					1372:1377	a Slt2- and Wsc1/2-independent manner	1341:1377	a Slt2- and Wsc1/2-independent manner	1341:1377	On the other hand, the ERG9-repressed sur1∆ csh1∆ cells also exhibited an increase in the cell wall chitin level in a Slt2- and Wsc1/2-independent manner.
29775232	6	30	theme	SLT2	1098:1101	arg1	deletion					1086:1093	the deletion	1082:1093	the deletion of SLT2 or WSC1/2, encoding a sensor protein recognizing CWI,	1082:1155	Moreover, the deletion of SLT2 or WSC1/2, encoding a sensor protein recognizing CWI, enhanced the growth defect in the ERG9-repressed sur1∆ csh1∆ cells.
29775232	8	31	theme	CWI	1520:1522	arg1	defect					1524:1529	the CWI defect	1516:1529	the CWI defect caused by these metabolic defects	1516:1563	These results suggested that MIPC and ergosterol are coordinately involved in maintenance of CWI, and the activation of Slt2 suppressed the CWI defect caused by these metabolic defects.
29775232	0	32	theme	Mannosylinositol	0:15	arg1	phosphorylceramides					17:35	Mannosylinositol phosphorylceramides	0:35	Mannosylinositol phosphorylceramides	0:35	Mannosylinositol phosphorylceramides and ergosterol coodinately maintain cell wall integrity in the yeast Saccharomyces cerevisiae.
29775232	8	33	theme	CWI	1473:1475	arg1	maintenance					1458:1468	maintenance	1458:1468	maintenance of CWI	1458:1475	These results suggested that MIPC and ergosterol are coordinately involved in maintenance of CWI, and the activation of Slt2 suppressed the CWI defect caused by these metabolic defects.
29775232	6	34	theme	growth	1170:1175	arg1	defect					1177:1182	the growth defect	1166:1182	the growth defect in the ERG9-repressed sur1∆ csh1∆ cells	1166:1222	Moreover, the deletion of SLT2 or WSC1/2, encoding a sensor protein recognizing CWI, enhanced the growth defect in the ERG9-repressed sur1∆ csh1∆ cells.
29775232	4	35	theme	expression	704:713	arg1	repression					690:699	repression	690:699	repression of expression of ERG9, which encodes squalene synthase in the ergosterol biosynthesis pathway, in sur1∆ csh1∆ cells	690:815	In addition, repression of expression of ERG9, which encodes squalene synthase in the ergosterol biosynthesis pathway, in sur1∆ csh1∆ cells caused a strong growth defect and enhancement of the defect in CWI.
29775232	7	36	from	increase	1299:1306	arg1	level					1332:1336	the cell wall chitin level	1311:1336	the cell wall chitin level	1311:1336	On the other hand, the ERG9-repressed sur1∆ csh1∆ cells also exhibited an increase in the cell wall chitin level in a Slt2- and Wsc1/2-independent manner.
29775232	1	37	theme	polar	213:217	arg1	group					224:228	polar head group	213:228	polar head group	213:228	In the yeast Saccharomyces cerevisiae, complex sphingolipids have three types of polar head group, and breakdown of their normal composition causes several cellular dysfunctions.
29775232	6	38	theme	sensor	1125:1130	arg1	protein					1132:1138	a sensor protein	1123:1138	a sensor protein recognizing CWI	1123:1154	Moreover, the deletion of SLT2 or WSC1/2, encoding a sensor protein recognizing CWI, enhanced the growth defect in the ERG9-repressed sur1∆ csh1∆ cells.
29775232	4	39	from	expression	704:713	arg1	cells					811:815	sur1∆ csh1∆ cells	799:815	sur1∆ csh1∆ cells	799:815	In addition, repression of expression of ERG9, which encodes squalene synthase in the ergosterol biosynthesis pathway, in sur1∆ csh1∆ cells caused a strong growth defect and enhancement of the defect in CWI.
29775232	5	40	theme	ERG9	903:906	arg1	repression					889:898	The repression	885:898	The repression of ERG9	885:906	The repression of ERG9 and/or the deletion of SUR1 and CSH1 caused an increase in the phosphorylated form of Slt2, a mitogen-activated protein kinase activated through impairment of CWI.
29775232	1	41	theme	head	219:222	arg1	group					224:228	polar head group	213:228	polar head group	213:228	In the yeast Saccharomyces cerevisiae, complex sphingolipids have three types of polar head group, and breakdown of their normal composition causes several cellular dysfunctions.
29775232	7	42	theme	chitin	1325:1330	arg1	level					1332:1336	the cell wall chitin level	1311:1336	the cell wall chitin level	1311:1336	On the other hand, the ERG9-repressed sur1∆ csh1∆ cells also exhibited an increase in the cell wall chitin level in a Slt2- and Wsc1/2-independent manner.
29775232	3	43	theme	MIPC	554:557	arg1	Csh1					579:582	Csh1	579:582	Csh1	579:582	In this study, we screened for multicopy suppressor genes that rescue the defect in CWI in cells lacking MIPC synthases (Sur1 and Csh1), and found that the defect is partly suppressed by upregulation of ergosterol biosynthesis.
29775232	3	43	theme	MIPC	554:557	arg1	Sur1					570:573	Sur1	570:573	Sur1	570:573	In this study, we screened for multicopy suppressor genes that rescue the defect in CWI in cells lacking MIPC synthases (Sur1 and Csh1), and found that the defect is partly suppressed by upregulation of ergosterol biosynthesis.
29775232	3	43	theme	MIPC	554:557	arg1	synthases					559:567	MIPC synthases	554:567	MIPC synthases (Sur1 and Csh1)	554:583	In this study, we screened for multicopy suppressor genes that rescue the defect in CWI in cells lacking MIPC synthases (Sur1 and Csh1), and found that the defect is partly suppressed by upregulation of ergosterol biosynthesis.
29775232	5	44	from	increase	955:962	arg1	form					986:989	the phosphorylated form	967:989	the phosphorylated form of Slt2, a mitogen-activated protein kinase activated through impairment of CWI	967:1069	The repression of ERG9 and/or the deletion of SUR1 and CSH1 caused an increase in the phosphorylated form of Slt2, a mitogen-activated protein kinase activated through impairment of CWI.
29775232	1	45	theme	group	224:228	arg1	types					204:208	three types	198:208	three types of polar head group	198:228	In the yeast Saccharomyces cerevisiae, complex sphingolipids have three types of polar head group, and breakdown of their normal composition causes several cellular dysfunctions.
29775232	2	46	theme	mannosylinositol	360:375	arg1	MIPC					397:400	MIPC	397:400	MIPC	397:400	Previously we found that loss of biosynthesis of mannosylinositol phosphorylceramide (MIPC) causes a defect in cell wall integrity (CWI).
29775232	2	46	theme	mannosylinositol	360:375	arg1	phosphorylceramide					377:394	mannosylinositol phosphorylceramide	360:394	mannosylinositol phosphorylceramide (MIPC)	360:401	Previously we found that loss of biosynthesis of mannosylinositol phosphorylceramide (MIPC) causes a defect in cell wall integrity (CWI).
29775232	5	47	theme	mitogen-activated	1002:1018	arg1	Slt2					994:997	Slt2	994:997	Slt2	994:997	The repression of ERG9 and/or the deletion of SUR1 and CSH1 caused an increase in the phosphorylated form of Slt2, a mitogen-activated protein kinase activated through impairment of CWI.
29775232	5	47	theme	mitogen-activated	1002:1018	arg1	kinase					1028:1033	a mitogen-activated protein kinase	1000:1033	a mitogen-activated protein kinase activated through impairment of CWI	1000:1069	The repression of ERG9 and/or the deletion of SUR1 and CSH1 caused an increase in the phosphorylated form of Slt2, a mitogen-activated protein kinase activated through impairment of CWI.
29775232	6	48	theme	csh1∆	1212:1216	arg1	cells					1218:1222	the ERG9-repressed sur1∆ csh1∆ cells	1187:1222	the ERG9-repressed sur1∆ csh1∆ cells	1187:1222	Moreover, the deletion of SLT2 or WSC1/2, encoding a sensor protein recognizing CWI, enhanced the growth defect in the ERG9-repressed sur1∆ csh1∆ cells.
29775232	4	49	from	CWI	880:882	arg1	enhancement					851:861	enhancement	851:861	enhancement of the defect in CWI	851:882	In addition, repression of expression of ERG9, which encodes squalene synthase in the ergosterol biosynthesis pathway, in sur1∆ csh1∆ cells caused a strong growth defect and enhancement of the defect in CWI.
29775232	4	49	from	CWI	880:882	arg1	defect					840:845	a strong growth defect	824:845	a strong growth defect	824:845	In addition, repression of expression of ERG9, which encodes squalene synthase in the ergosterol biosynthesis pathway, in sur1∆ csh1∆ cells caused a strong growth defect and enhancement of the defect in CWI.
29775232	2	50	from	defect	412:417	arg1	integrity					432:440	cell wall integrity	422:440	cell wall integrity (CWI)	422:446	Previously we found that loss of biosynthesis of mannosylinositol phosphorylceramide (MIPC) causes a defect in cell wall integrity (CWI).
29775232	2	50	from	defect	412:417	arg1	CWI					443:445	CWI	443:445	CWI	443:445	Previously we found that loss of biosynthesis of mannosylinositol phosphorylceramide (MIPC) causes a defect in cell wall integrity (CWI).
29775232	2	51	theme	wall	427:430	arg1	integrity					432:440	cell wall integrity	422:440	cell wall integrity (CWI)	422:446	Previously we found that loss of biosynthesis of mannosylinositol phosphorylceramide (MIPC) causes a defect in cell wall integrity (CWI).
29775232	2	51	theme	wall	427:430	arg1	CWI					443:445	CWI	443:445	CWI	443:445	Previously we found that loss of biosynthesis of mannosylinositol phosphorylceramide (MIPC) causes a defect in cell wall integrity (CWI).
29775232	0	52	theme	wall	78:81	arg1	integrity					83:91	cell wall integrity	73:91	cell wall integrity	73:91	Mannosylinositol phosphorylceramides and ergosterol coodinately maintain cell wall integrity in the yeast Saccharomyces cerevisiae.
29775232	4	53	theme	sur1∆	799:803	arg1	cells					811:815	sur1∆ csh1∆ cells	799:815	sur1∆ csh1∆ cells	799:815	In addition, repression of expression of ERG9, which encodes squalene synthase in the ergosterol biosynthesis pathway, in sur1∆ csh1∆ cells caused a strong growth defect and enhancement of the defect in CWI.
29775232	2	54	theme	phosphorylceramide	377:394	arg1	biosynthesis					344:355	biosynthesis	344:355	biosynthesis of mannosylinositol phosphorylceramide (MIPC)	344:401	Previously we found that loss of biosynthesis of mannosylinositol phosphorylceramide (MIPC) causes a defect in cell wall integrity (CWI).
29775232	0	55	theme	cell	73:76	arg1	integrity					83:91	cell wall integrity	73:91	cell wall integrity	73:91	Mannosylinositol phosphorylceramides and ergosterol coodinately maintain cell wall integrity in the yeast Saccharomyces cerevisiae.
29775232	8	56	theme	Slt2	1500:1503	arg1	activation					1486:1495	the activation	1482:1495	the activation of Slt2	1482:1503	These results suggested that MIPC and ergosterol are coordinately involved in maintenance of CWI, and the activation of Slt2 suppressed the CWI defect caused by these metabolic defects.
29775232	4	57	from	defect	870:875	arg1	CWI					880:882	CWI	880:882	CWI	880:882	In addition, repression of expression of ERG9, which encodes squalene synthase in the ergosterol biosynthesis pathway, in sur1∆ csh1∆ cells caused a strong growth defect and enhancement of the defect in CWI.
29775232	5	58	theme	phosphorylated	971:984	arg1	form					986:989	the phosphorylated form	967:989	the phosphorylated form of Slt2, a mitogen-activated protein kinase activated through impairment of CWI	967:1069	The repression of ERG9 and/or the deletion of SUR1 and CSH1 caused an increase in the phosphorylated form of Slt2, a mitogen-activated protein kinase activated through impairment of CWI.
29775232	7	59	theme	ERG9-repressed	1248:1261	arg1	cells					1275:1279	the ERG9-repressed sur1∆ csh1∆ cells	1244:1279	the ERG9-repressed sur1∆ csh1∆ cells	1244:1279	On the other hand, the ERG9-repressed sur1∆ csh1∆ cells also exhibited an increase in the cell wall chitin level in a Slt2- and Wsc1/2-independent manner.
29775232	1	60	theme	yeast	139:143	arg1	cerevisiae					159:168	the yeast Saccharomyces cerevisiae	135:168	the yeast Saccharomyces cerevisiae	135:168	In the yeast Saccharomyces cerevisiae, complex sphingolipids have three types of polar head group, and breakdown of their normal composition causes several cellular dysfunctions.
29775232	7	61	theme	csh1∆	1269:1273	arg1	cells					1275:1279	the ERG9-repressed sur1∆ csh1∆ cells	1244:1279	the ERG9-repressed sur1∆ csh1∆ cells	1244:1279	On the other hand, the ERG9-repressed sur1∆ csh1∆ cells also exhibited an increase in the cell wall chitin level in a Slt2- and Wsc1/2-independent manner.
29775232	4	62	theme	defect	870:875	arg1	enhancement					851:861	enhancement	851:861	enhancement of the defect in CWI	851:882	In addition, repression of expression of ERG9, which encodes squalene synthase in the ergosterol biosynthesis pathway, in sur1∆ csh1∆ cells caused a strong growth defect and enhancement of the defect in CWI.
29775232	4	62	theme	defect	870:875	arg1	defect					840:845	a strong growth defect	824:845	a strong growth defect	824:845	In addition, repression of expression of ERG9, which encodes squalene synthase in the ergosterol biosynthesis pathway, in sur1∆ csh1∆ cells caused a strong growth defect and enhancement of the defect in CWI.
29775232	1	63	theme	Saccharomyces	145:157	arg1	cerevisiae					159:168	the yeast Saccharomyces cerevisiae	135:168	the yeast Saccharomyces cerevisiae	135:168	In the yeast Saccharomyces cerevisiae, complex sphingolipids have three types of polar head group, and breakdown of their normal composition causes several cellular dysfunctions.
29775232	4	64	from	repression	690:699	arg1	cells					811:815	sur1∆ csh1∆ cells	799:815	sur1∆ csh1∆ cells	799:815	In addition, repression of expression of ERG9, which encodes squalene synthase in the ergosterol biosynthesis pathway, in sur1∆ csh1∆ cells caused a strong growth defect and enhancement of the defect in CWI.
29775232	7	65	theme	cell	1315:1318	arg1	level					1332:1336	the cell wall chitin level	1311:1336	the cell wall chitin level	1311:1336	On the other hand, the ERG9-repressed sur1∆ csh1∆ cells also exhibited an increase in the cell wall chitin level in a Slt2- and Wsc1/2-independent manner.
29775232	5	66	theme	SUR1	931:934	arg1	deletion					919:926	the deletion	915:926	the deletion of SUR1 and CSH1	915:943	The repression of ERG9 and/or the deletion of SUR1 and CSH1 caused an increase in the phosphorylated form of Slt2, a mitogen-activated protein kinase activated through impairment of CWI.
29775232	5	67	theme	Slt2	994:997	arg1	form					986:989	the phosphorylated form	967:989	the phosphorylated form of Slt2, a mitogen-activated protein kinase activated through impairment of CWI	967:1069	The repression of ERG9 and/or the deletion of SUR1 and CSH1 caused an increase in the phosphorylated form of Slt2, a mitogen-activated protein kinase activated through impairment of CWI.
29775232	5	68	theme	CSH1	940:943	arg1	deletion					919:926	the deletion	915:926	the deletion of SUR1 and CSH1	915:943	The repression of ERG9 and/or the deletion of SUR1 and CSH1 caused an increase in the phosphorylated form of Slt2, a mitogen-activated protein kinase activated through impairment of CWI.
29775232	2	69	theme	biosynthesis	344:355	arg1	loss					336:339	loss	336:339	loss of biosynthesis of mannosylinositol phosphorylceramide (MIPC)	336:401	Previously we found that loss of biosynthesis of mannosylinositol phosphorylceramide (MIPC) causes a defect in cell wall integrity (CWI).
29775232	4	70	from	enhancement	851:861	arg1	CWI					880:882	CWI	880:882	CWI	880:882	In addition, repression of expression of ERG9, which encodes squalene synthase in the ergosterol biosynthesis pathway, in sur1∆ csh1∆ cells caused a strong growth defect and enhancement of the defect in CWI.
29775232	4	71	theme	biosynthesis	774:785	arg1	pathway					787:793	the ergosterol biosynthesis pathway	759:793	the ergosterol biosynthesis pathway	759:793	In addition, repression of expression of ERG9, which encodes squalene synthase in the ergosterol biosynthesis pathway, in sur1∆ csh1∆ cells caused a strong growth defect and enhancement of the defect in CWI.
29775232	3	72	theme	multicopy	480:488	arg1	genes					501:505	multicopy suppressor genes	480:505	multicopy suppressor genes that rescue the defect in CWI in cells lacking MIPC synthases (Sur1 and Csh1)	480:583	In this study, we screened for multicopy suppressor genes that rescue the defect in CWI in cells lacking MIPC synthases (Sur1 and Csh1), and found that the defect is partly suppressed by upregulation of ergosterol biosynthesis.
29775232	8	73	theme	metabolic	1547:1555	arg1	defects					1557:1563	these metabolic defects	1541:1563	these metabolic defects	1541:1563	These results suggested that MIPC and ergosterol are coordinately involved in maintenance of CWI, and the activation of Slt2 suppressed the CWI defect caused by these metabolic defects.
29775232	5	74	theme	CWI	1067:1069	arg1	impairment					1053:1062	impairment	1053:1062	impairment of CWI	1053:1069	The repression of ERG9 and/or the deletion of SUR1 and CSH1 caused an increase in the phosphorylated form of Slt2, a mitogen-activated protein kinase activated through impairment of CWI.
29775232	4	75	theme	ergosterol	763:772	arg1	pathway					787:793	the ergosterol biosynthesis pathway	759:793	the ergosterol biosynthesis pathway	759:793	In addition, repression of expression of ERG9, which encodes squalene synthase in the ergosterol biosynthesis pathway, in sur1∆ csh1∆ cells caused a strong growth defect and enhancement of the defect in CWI.
29775232	1	76	theme	complex	171:177	arg1	sphingolipids					179:191	complex sphingolipids	171:191	complex sphingolipids	171:191	In the yeast Saccharomyces cerevisiae, complex sphingolipids have three types of polar head group, and breakdown of their normal composition causes several cellular dysfunctions.
29775232	1	77	theme	normal	254:259	arg1	composition					261:271	their normal composition	248:271	their normal composition	248:271	In the yeast Saccharomyces cerevisiae, complex sphingolipids have three types of polar head group, and breakdown of their normal composition causes several cellular dysfunctions.
29748675	7	0	theme	upregulated	1434:1444	arg1	genes					1446:1450	seven upregulated genes	1428:1450	seven upregulated genes	1428:1450	and an unknown genus of YS2, and the decreased Lachnospira sp., Oscillospira sp., Ruminococcus sp., an unknown genus of Peptococcaceae and an unknown genus of Clostridiales in rats' cecum-and eight genes-including one downregulated Pla2g2a and seven upregulated genes: Apoa1, Apoa4, Aabr07073400.1, Fabp4, Pik3r5, Dgat2 and Ptgs2 of colonic epithelial cells-that were due to the consumption of catechin.
29748675	7	1	theme	unknown	1191:1197	arg1	genus					1199:1203	an unknown genus	1188:1203	an unknown genus of YS2	1188:1210	and an unknown genus of YS2, and the decreased Lachnospira sp., Oscillospira sp., Ruminococcus sp., an unknown genus of Peptococcaceae and an unknown genus of Clostridiales in rats' cecum-and eight genes-including one downregulated Pla2g2a and seven upregulated genes: Apoa1, Apoa4, Aabr07073400.1, Fabp4, Pik3r5, Dgat2 and Ptgs2 of colonic epithelial cells-that were due to the consumption of catechin.
29748675	9	2	theme	weight	1833:1838	arg1	loss					1840:1843	host weight loss	1828:1843	host weight loss	1828:1843	In conclusion, catechin induces host weight loss by altering gut microbiota and gene expression and function in colonic epithelial cells.
29748675	3	3	theme	d-1	679:681	arg1	catechin					683:690	kg-1 d-1 catechin	674:690	100, 400, and 700 mg kg-1 d-1 catechin	653:690	Forty obese rats were divided into four groups: three catechin-supplemented groups with a high-FOS diet (100, 400, and 700 mg kg-1 d-1 catechin, orally administered) and one group with a high-FOS diet only.
29748675	3	4	with	diet	647:650	arg1	diet					744:747	a high-FOS diet	733:747	a high-FOS diet only	733:752	Forty obese rats were divided into four groups: three catechin-supplemented groups with a high-FOS diet (100, 400, and 700 mg kg-1 d-1 catechin, orally administered) and one group with a high-FOS diet only.
29748675	8	5	theme	energy	1650:1655	arg1	metabolism					1657:1666	energy metabolism	1650:1666	energy metabolism in colonic epithelial cells	1650:1694	Consequently, various biological functions in connection with energy metabolism in colonic epithelial cells were altered, including fat digestion and absorption and the regulation of lipolysis in adipocytes.
29748675	9	6	theme	gut	1857:1859	arg1	microbiota					1861:1870	gut microbiota and gene expression	1857:1890	microbiota	1861:1870	In conclusion, catechin induces host weight loss by altering gut microbiota and gene expression and function in colonic epithelial cells.
29748675	3	7	with	groups	624:629	arg1	group					722:726	one group	718:726	one group with a high-FOS diet only	718:752	Forty obese rats were divided into four groups: three catechin-supplemented groups with a high-FOS diet (100, 400, and 700 mg kg-1 d-1 catechin, orally administered) and one group with a high-FOS diet only.
29748675	3	7	with	groups	624:629	arg1	diet					647:650	a high-FOS diet	636:650	a high-FOS diet (100, 400, and 700 mg kg-1 d-1 catechin, orally administered)	636:712	Forty obese rats were divided into four groups: three catechin-supplemented groups with a high-FOS diet (100, 400, and 700 mg kg-1 d-1 catechin, orally administered) and one group with a high-FOS diet only.
29748675	2	8	theme	cells	541:545	arg1	expression					508:517	the expression	504:517	the expression of colonic epithelial cells	504:545	This experiment is devised to further confirm the relationship between specific bacteria in the colon and body weight gain, and to investigate how specific bacteria impact body weight by changing the expression of colonic epithelial cells.
29748675	7	9	theme	unknown	1326:1332	arg1	genus					1334:1338	an unknown genus	1323:1338	an unknown genus of Clostridiales in rats	1323:1363	and an unknown genus of YS2, and the decreased Lachnospira sp., Oscillospira sp., Ruminococcus sp., an unknown genus of Peptococcaceae and an unknown genus of Clostridiales in rats' cecum-and eight genes-including one downregulated Pla2g2a and seven upregulated genes: Apoa1, Apoa4, Aabr07073400.1, Fabp4, Pik3r5, Dgat2 and Ptgs2 of colonic epithelial cells-that were due to the consumption of catechin.
29748675	6	10	dep	Robinsoniella	1123:1135	arg1	sp.					1137:1139	Robinsoniella sp.	1123:1139	Robinsoniella sp.	1123:1139	Results identified some specific bacteria at the genus level-including the increased Parabacteroides sp., Prevotella sp., Robinsoniella sp., [Ruminococcus], Phascolarctobacterium sp.
29748675	8	11	theme	epithelial	1679:1688	arg1	cells					1690:1694	colonic epithelial cells	1671:1694	colonic epithelial cells	1671:1694	Consequently, various biological functions in connection with energy metabolism in colonic epithelial cells were altered, including fat digestion and absorption and the regulation of lipolysis in adipocytes.
29748675	2	12	theme	colonic	522:528	arg1	cells					541:545	colonic epithelial cells	522:545	colonic epithelial cells	522:545	This experiment is devised to further confirm the relationship between specific bacteria in the colon and body weight gain, and to investigate how specific bacteria impact body weight by changing the expression of colonic epithelial cells.
29748675	0	13	theme	epithelial	116:125	arg1	cells					127:131	colonic epithelial cells	108:131	colonic epithelial cells	108:131	Catechin supplemented in a FOS diet induces weight loss by altering cecal microbiota and gene expression of colonic epithelial cells.
29748675	9	14	from	microbiota	1861:1870	arg1	cells					1927:1931	colonic epithelial cells	1908:1931	colonic epithelial cells	1908:1931	In conclusion, catechin induces host weight loss by altering gut microbiota and gene expression and function in colonic epithelial cells.
29748675	8	15	from	regulation	1757:1766	arg1	adipocytes					1784:1793	adipocytes	1784:1793	adipocytes	1784:1793	Consequently, various biological functions in connection with energy metabolism in colonic epithelial cells were altered, including fat digestion and absorption and the regulation of lipolysis in adipocytes.
29748675	7	16	theme	epithelial	1525:1534	arg1	cells-that					1536:1545	colonic epithelial cells-that	1517:1545	colonic epithelial cells-that	1517:1545	and an unknown genus of YS2, and the decreased Lachnospira sp., Oscillospira sp., Ruminococcus sp., an unknown genus of Peptococcaceae and an unknown genus of Clostridiales in rats' cecum-and eight genes-including one downregulated Pla2g2a and seven upregulated genes: Apoa1, Apoa4, Aabr07073400.1, Fabp4, Pik3r5, Dgat2 and Ptgs2 of colonic epithelial cells-that were due to the consumption of catechin.
29748675	6	17	theme	Phascolarctobacterium	1158:1178	arg1	[Ruminococcus					1142:1154	the increased Parabacteroides sp., Prevotella sp., Robinsoniella sp., [Ruminococcus]	1072:1155	the increased Parabacteroides sp., Prevotella sp., Robinsoniella sp., [Ruminococcus]	1072:1155	Results identified some specific bacteria at the genus level-including the increased Parabacteroides sp., Prevotella sp., Robinsoniella sp., [Ruminococcus], Phascolarctobacterium sp.
29748675	6	17	theme	Phascolarctobacterium	1158:1178	arg1	sp					1180:1181	Phascolarctobacterium sp	1158:1181	Phascolarctobacterium sp	1158:1181	Results identified some specific bacteria at the genus level-including the increased Parabacteroides sp., Prevotella sp., Robinsoniella sp., [Ruminococcus], Phascolarctobacterium sp.
29748675	7	18	theme	unknown	1287:1293	arg1	Oscillospira					1248:1259	Oscillospira	1248:1259	Oscillospira	1248:1259	and an unknown genus of YS2, and the decreased Lachnospira sp., Oscillospira sp., Ruminococcus sp., an unknown genus of Peptococcaceae and an unknown genus of Clostridiales in rats' cecum-and eight genes-including one downregulated Pla2g2a and seven upregulated genes: Apoa1, Apoa4, Aabr07073400.1, Fabp4, Pik3r5, Dgat2 and Ptgs2 of colonic epithelial cells-that were due to the consumption of catechin.
29748675	7	18	theme	unknown	1287:1293	arg1	genus					1295:1299	an unknown genus	1284:1299	an unknown genus of Peptococcaceae	1284:1317	and an unknown genus of YS2, and the decreased Lachnospira sp., Oscillospira sp., Ruminococcus sp., an unknown genus of Peptococcaceae and an unknown genus of Clostridiales in rats' cecum-and eight genes-including one downregulated Pla2g2a and seven upregulated genes: Apoa1, Apoa4, Aabr07073400.1, Fabp4, Pik3r5, Dgat2 and Ptgs2 of colonic epithelial cells-that were due to the consumption of catechin.
29748675	9	19	theme	colonic	1908:1914	arg1	cells					1927:1931	colonic epithelial cells	1908:1931	colonic epithelial cells	1908:1931	In conclusion, catechin induces host weight loss by altering gut microbiota and gene expression and function in colonic epithelial cells.
29748675	3	20	theme	catechin-supplemented	602:622	arg1	groups					624:629	three catechin-supplemented groups	596:629	four groups: three catechin-supplemented groups with a high-FOS diet (100, 400, and 700 mg kg-1 d-1 catechin, orally administered) and one group with a high-FOS diet only	583:752	Forty obese rats were divided into four groups: three catechin-supplemented groups with a high-FOS diet (100, 400, and 700 mg kg-1 d-1 catechin, orally administered) and one group with a high-FOS diet only.
29748675	8	21	theme	fat	1720:1722	arg1	digestion					1724:1732	fat digestion	1720:1732	fat digestion	1720:1732	Consequently, various biological functions in connection with energy metabolism in colonic epithelial cells were altered, including fat digestion and absorption and the regulation of lipolysis in adipocytes.
29748675	7	22	dep	cecum-and	1366:1374	arg1	genes-including					1382:1396	eight genes-including one	1376:1400	the decreased Lachnospira sp., Oscillospira sp., Ruminococcus sp., an unknown genus of Peptococcaceae and an unknown genus of Clostridiales in rats' cecum-and eight genes-including one	1217:1400	and an unknown genus of YS2, and the decreased Lachnospira sp., Oscillospira sp., Ruminococcus sp., an unknown genus of Peptococcaceae and an unknown genus of Clostridiales in rats' cecum-and eight genes-including one downregulated Pla2g2a and seven upregulated genes: Apoa1, Apoa4, Aabr07073400.1, Fabp4, Pik3r5, Dgat2 and Ptgs2 of colonic epithelial cells-that were due to the consumption of catechin.
29748675	8	23	from	adipocytes	1784:1793	arg1	absorption					1738:1747	absorption	1738:1747	absorption	1738:1747	Consequently, various biological functions in connection with energy metabolism in colonic epithelial cells were altered, including fat digestion and absorption and the regulation of lipolysis in adipocytes.
29748675	8	23	from	adipocytes	1784:1793	arg1	regulation					1757:1766	the regulation	1753:1766	the regulation of lipolysis in adipocytes	1753:1793	Consequently, various biological functions in connection with energy metabolism in colonic epithelial cells were altered, including fat digestion and absorption and the regulation of lipolysis in adipocytes.
29748675	8	23	from	adipocytes	1784:1793	arg1	digestion					1724:1732	fat digestion	1720:1732	fat digestion	1720:1732	Consequently, various biological functions in connection with energy metabolism in colonic epithelial cells were altered, including fat digestion and absorption and the regulation of lipolysis in adipocytes.
29748675	8	24	from	lipolysis	1771:1779	arg1	adipocytes					1784:1793	adipocytes	1784:1793	adipocytes	1784:1793	Consequently, various biological functions in connection with energy metabolism in colonic epithelial cells were altered, including fat digestion and absorption and the regulation of lipolysis in adipocytes.
29748675	5	25	theme	treatment	833:841	arg1	month					824:828	one month	820:828	one month of treatment	820:841	After one month of treatment, rats' cecal content and colonic epithelial cells were individually collected and analyzed with MiSeq and gene expression profiling techniques, respectively.
29748675	8	26	with	connection	1634:1643	arg1	metabolism					1657:1666	energy metabolism	1650:1666	energy metabolism in colonic epithelial cells	1650:1694	Consequently, various biological functions in connection with energy metabolism in colonic epithelial cells were altered, including fat digestion and absorption and the regulation of lipolysis in adipocytes.
29748675	3	27	with	group	722:726	arg1	diet					744:747	a high-FOS diet	733:747	a high-FOS diet only	733:752	Forty obese rats were divided into four groups: three catechin-supplemented groups with a high-FOS diet (100, 400, and 700 mg kg-1 d-1 catechin, orally administered) and one group with a high-FOS diet only.
29748675	5	28	theme	cecal	850:854	arg1	content					856:862	rats' cecal content	844:862	rats' cecal content	844:862	After one month of treatment, rats' cecal content and colonic epithelial cells were individually collected and analyzed with MiSeq and gene expression profiling techniques, respectively.
29748675	0	29	theme	gene	89:92	arg1	expression					94:103	gene expression	89:103	gene expression	89:103	Catechin supplemented in a FOS diet induces weight loss by altering cecal microbiota and gene expression of colonic epithelial cells.
29748675	8	30	theme	lipolysis	1771:1779	arg1	absorption					1738:1747	absorption	1738:1747	absorption	1738:1747	Consequently, various biological functions in connection with energy metabolism in colonic epithelial cells were altered, including fat digestion and absorption and the regulation of lipolysis in adipocytes.
29748675	8	30	theme	lipolysis	1771:1779	arg1	regulation					1757:1766	the regulation	1753:1766	the regulation of lipolysis in adipocytes	1753:1793	Consequently, various biological functions in connection with energy metabolism in colonic epithelial cells were altered, including fat digestion and absorption and the regulation of lipolysis in adipocytes.
29748675	8	30	theme	lipolysis	1771:1779	arg1	digestion					1724:1732	fat digestion	1720:1732	fat digestion	1720:1732	Consequently, various biological functions in connection with energy metabolism in colonic epithelial cells were altered, including fat digestion and absorption and the regulation of lipolysis in adipocytes.
29748675	8	31	from	functions	1621:1629	arg1	connection					1634:1643	connection	1634:1643	connection with energy metabolism in colonic epithelial cells	1634:1694	Consequently, various biological functions in connection with energy metabolism in colonic epithelial cells were altered, including fat digestion and absorption and the regulation of lipolysis in adipocytes.
29748675	2	32	theme	specific	379:386	arg1	bacteria					388:395	specific bacteria	379:395	specific bacteria in the colon and body weight gain	379:429	This experiment is devised to further confirm the relationship between specific bacteria in the colon and body weight gain, and to investigate how specific bacteria impact body weight by changing the expression of colonic epithelial cells.
29748675	2	33	dep	colon	404:408	arg1	the					400:402	the	400:402	the	400:402	This experiment is devised to further confirm the relationship between specific bacteria in the colon and body weight gain, and to investigate how specific bacteria impact body weight by changing the expression of colonic epithelial cells.
29748675	2	34	theme	body	414:417	arg1	gain					426:429	body weight gain	414:429	body weight gain	414:429	This experiment is devised to further confirm the relationship between specific bacteria in the colon and body weight gain, and to investigate how specific bacteria impact body weight by changing the expression of colonic epithelial cells.
29748675	5	35	theme	epithelial	876:885	arg1	cells					887:891	colonic epithelial cells	868:891	colonic epithelial cells	868:891	After one month of treatment, rats' cecal content and colonic epithelial cells were individually collected and analyzed with MiSeq and gene expression profiling techniques, respectively.
29748675	2	36	theme	specific	455:462	arg1	bacteria					464:471	specific bacteria	455:471	specific bacteria	455:471	This experiment is devised to further confirm the relationship between specific bacteria in the colon and body weight gain, and to investigate how specific bacteria impact body weight by changing the expression of colonic epithelial cells.
29748675	3	37	theme	high-FOS	638:645	arg1	diet					647:650	a high-FOS diet	636:650	a high-FOS diet (100, 400, and 700 mg kg-1 d-1 catechin, orally administered)	636:712	Forty obese rats were divided into four groups: three catechin-supplemented groups with a high-FOS diet (100, 400, and 700 mg kg-1 d-1 catechin, orally administered) and one group with a high-FOS diet only.
29748675	4	38	theme	Food	755:758	arg1	consumption					760:770	Food consumption	755:770	Food consumption	755:770	Food consumption and body weights were recorded each week.
29748675	0	39	theme	FOS	27:29	arg1	diet					31:34	a FOS diet	25:34	a FOS diet	25:34	Catechin supplemented in a FOS diet induces weight loss by altering cecal microbiota and gene expression of colonic epithelial cells.
29748675	3	40	dep	mg	671:672	arg1	catechin					683:690	kg-1 d-1 catechin	674:690	100, 400, and 700 mg kg-1 d-1 catechin	653:690	Forty obese rats were divided into four groups: three catechin-supplemented groups with a high-FOS diet (100, 400, and 700 mg kg-1 d-1 catechin, orally administered) and one group with a high-FOS diet only.
29748675	5	41	theme	gene	949:952	arg1	techniques					975:984	gene expression profiling techniques	949:984	gene expression profiling techniques	949:984	After one month of treatment, rats' cecal content and colonic epithelial cells were individually collected and analyzed with MiSeq and gene expression profiling techniques, respectively.
29748675	9	42	from	function	1896:1903	arg1	cells					1927:1931	colonic epithelial cells	1908:1931	colonic epithelial cells	1908:1931	In conclusion, catechin induces host weight loss by altering gut microbiota and gene expression and function in colonic epithelial cells.
29748675	5	43	theme	profiling	965:973	arg1	techniques					975:984	gene expression profiling techniques	949:984	gene expression profiling techniques	949:984	After one month of treatment, rats' cecal content and colonic epithelial cells were individually collected and analyzed with MiSeq and gene expression profiling techniques, respectively.
29748675	6	44	theme	increased	1076:1084	arg1	[Ruminococcus					1142:1154	the increased Parabacteroides sp., Prevotella sp., Robinsoniella sp., [Ruminococcus]	1072:1155	the increased Parabacteroides sp., Prevotella sp., Robinsoniella sp., [Ruminococcus]	1072:1155	Results identified some specific bacteria at the genus level-including the increased Parabacteroides sp., Prevotella sp., Robinsoniella sp., [Ruminococcus], Phascolarctobacterium sp.
29748675	6	44	theme	increased	1076:1084	arg1	sp					1180:1181	Phascolarctobacterium sp	1158:1181	Phascolarctobacterium sp	1158:1181	Results identified some specific bacteria at the genus level-including the increased Parabacteroides sp., Prevotella sp., Robinsoniella sp., [Ruminococcus], Phascolarctobacterium sp.
29748675	6	45	dep	Parabacteroides	1086:1100	arg1	sp.					1102:1104	Parabacteroides sp.	1086:1104	Parabacteroides sp.	1086:1104	Results identified some specific bacteria at the genus level-including the increased Parabacteroides sp., Prevotella sp., Robinsoniella sp., [Ruminococcus], Phascolarctobacterium sp.
29748675	1	46	theme	gut	216:218	arg1	composition					231:241	gut microbiota composition	216:241	gut microbiota composition	216:241	Our previous study showed that catechin controlled rats' body weights and changed gut microbiota composition when supplemented into a high-fructo-oligosaccharide (FOS) diet.
29748675	7	47	dep	Oscillospira	1248:1259	arg1	sp.					1261:1263	Oscillospira sp., Ruminococcus sp., an unknown genus of Peptococcaceae	1248:1317	sp.	1261:1263	and an unknown genus of YS2, and the decreased Lachnospira sp., Oscillospira sp., Ruminococcus sp., an unknown genus of Peptococcaceae and an unknown genus of Clostridiales in rats' cecum-and eight genes-including one downregulated Pla2g2a and seven upregulated genes: Apoa1, Apoa4, Aabr07073400.1, Fabp4, Pik3r5, Dgat2 and Ptgs2 of colonic epithelial cells-that were due to the consumption of catechin.
29748675	7	47	dep	Oscillospira	1248:1259	arg1	sp.					1279:1281	Ruminococcus sp.	1266:1281	Ruminococcus sp.	1266:1281	and an unknown genus of YS2, and the decreased Lachnospira sp., Oscillospira sp., Ruminococcus sp., an unknown genus of Peptococcaceae and an unknown genus of Clostridiales in rats' cecum-and eight genes-including one downregulated Pla2g2a and seven upregulated genes: Apoa1, Apoa4, Aabr07073400.1, Fabp4, Pik3r5, Dgat2 and Ptgs2 of colonic epithelial cells-that were due to the consumption of catechin.
29748675	7	48	theme	YS2	1208:1210	arg1	genus					1199:1203	an unknown genus	1188:1203	an unknown genus of YS2	1188:1210	and an unknown genus of YS2, and the decreased Lachnospira sp., Oscillospira sp., Ruminococcus sp., an unknown genus of Peptococcaceae and an unknown genus of Clostridiales in rats' cecum-and eight genes-including one downregulated Pla2g2a and seven upregulated genes: Apoa1, Apoa4, Aabr07073400.1, Fabp4, Pik3r5, Dgat2 and Ptgs2 of colonic epithelial cells-that were due to the consumption of catechin.
29748675	7	48	theme	YS2	1208:1210	arg1	cecum-and					1366:1374	the decreased Lachnospira sp., Oscillospira sp., Ruminococcus sp., an unknown genus of Peptococcaceae and an unknown genus of Clostridiales in rats' cecum-and	1217:1374	the decreased Lachnospira sp., Oscillospira sp., Ruminococcus sp., an unknown genus of Peptococcaceae and an unknown genus of Clostridiales in rats' cecum-and eight genes-including one	1217:1400	and an unknown genus of YS2, and the decreased Lachnospira sp., Oscillospira sp., Ruminococcus sp., an unknown genus of Peptococcaceae and an unknown genus of Clostridiales in rats' cecum-and eight genes-including one downregulated Pla2g2a and seven upregulated genes: Apoa1, Apoa4, Aabr07073400.1, Fabp4, Pik3r5, Dgat2 and Ptgs2 of colonic epithelial cells-that were due to the consumption of catechin.
29748675	3	49	dep	diet	647:650	arg1	mg					671:672	100, 400, and 700 mg	653:672	100, 400, and 700 mg kg-1 d-1 catechin	653:690	Forty obese rats were divided into four groups: three catechin-supplemented groups with a high-FOS diet (100, 400, and 700 mg kg-1 d-1 catechin, orally administered) and one group with a high-FOS diet only.
29748675	9	50	theme	host	1828:1831	arg1	loss					1840:1843	host weight loss	1828:1843	host weight loss	1828:1843	In conclusion, catechin induces host weight loss by altering gut microbiota and gene expression and function in colonic epithelial cells.
29748675	8	51	theme	biological	1610:1619	arg1	functions					1621:1629	various biological functions	1602:1629	various biological functions in connection with energy metabolism in colonic epithelial cells	1602:1694	Consequently, various biological functions in connection with energy metabolism in colonic epithelial cells were altered, including fat digestion and absorption and the regulation of lipolysis in adipocytes.
29748675	4	52	theme	body	776:779	arg1	weights					781:787	body weights	776:787	body weights	776:787	Food consumption and body weights were recorded each week.
29748675	3	53	theme	kg-1	674:677	arg1	catechin					683:690	kg-1 d-1 catechin	674:690	100, 400, and 700 mg kg-1 d-1 catechin	653:690	Forty obese rats were divided into four groups: three catechin-supplemented groups with a high-FOS diet (100, 400, and 700 mg kg-1 d-1 catechin, orally administered) and one group with a high-FOS diet only.
29748675	3	54	theme	obese	554:558	arg1	rats					560:563	Forty obese rats	548:563	Forty obese rats	548:563	Forty obese rats were divided into four groups: three catechin-supplemented groups with a high-FOS diet (100, 400, and 700 mg kg-1 d-1 catechin, orally administered) and one group with a high-FOS diet only.
29748675	7	55	theme	Clostridiales	1343:1355	arg1	genus					1334:1338	an unknown genus	1323:1338	an unknown genus of Clostridiales in rats	1323:1363	and an unknown genus of YS2, and the decreased Lachnospira sp., Oscillospira sp., Ruminococcus sp., an unknown genus of Peptococcaceae and an unknown genus of Clostridiales in rats' cecum-and eight genes-including one downregulated Pla2g2a and seven upregulated genes: Apoa1, Apoa4, Aabr07073400.1, Fabp4, Pik3r5, Dgat2 and Ptgs2 of colonic epithelial cells-that were due to the consumption of catechin.
29748675	6	56	dep	Prevotella	1107:1116	arg1	sp.					1118:1120	Prevotella sp.	1107:1120	Prevotella sp.	1107:1120	Results identified some specific bacteria at the genus level-including the increased Parabacteroides sp., Prevotella sp., Robinsoniella sp., [Ruminococcus], Phascolarctobacterium sp.
29748675	0	57	theme	colonic	108:114	arg1	cells					127:131	colonic epithelial cells	108:131	colonic epithelial cells	108:131	Catechin supplemented in a FOS diet induces weight loss by altering cecal microbiota and gene expression of colonic epithelial cells.
29748675	3	58	dep	groups	588:593	arg1	groups					624:629	three catechin-supplemented groups	596:629	four groups: three catechin-supplemented groups with a high-FOS diet (100, 400, and 700 mg kg-1 d-1 catechin, orally administered) and one group with a high-FOS diet only	583:752	Forty obese rats were divided into four groups: three catechin-supplemented groups with a high-FOS diet (100, 400, and 700 mg kg-1 d-1 catechin, orally administered) and one group with a high-FOS diet only.
29748675	2	59	theme	epithelial	530:539	arg1	cells					541:545	colonic epithelial cells	522:545	colonic epithelial cells	522:545	This experiment is devised to further confirm the relationship between specific bacteria in the colon and body weight gain, and to investigate how specific bacteria impact body weight by changing the expression of colonic epithelial cells.
29748675	9	60	theme	gene	1876:1879	arg1	expression					1881:1890	gut microbiota and gene expression	1857:1890	expression	1881:1890	In conclusion, catechin induces host weight loss by altering gut microbiota and gene expression and function in colonic epithelial cells.
29748675	8	61	from	digestion	1724:1732	arg1	adipocytes					1784:1793	adipocytes	1784:1793	adipocytes	1784:1793	Consequently, various biological functions in connection with energy metabolism in colonic epithelial cells were altered, including fat digestion and absorption and the regulation of lipolysis in adipocytes.
29748675	7	62	theme	decreased	1221:1229	arg1	cecum-and					1366:1374	the decreased Lachnospira sp., Oscillospira sp., Ruminococcus sp., an unknown genus of Peptococcaceae and an unknown genus of Clostridiales in rats' cecum-and	1217:1374	the decreased Lachnospira sp., Oscillospira sp., Ruminococcus sp., an unknown genus of Peptococcaceae and an unknown genus of Clostridiales in rats' cecum-and eight genes-including one	1217:1400	and an unknown genus of YS2, and the decreased Lachnospira sp., Oscillospira sp., Ruminococcus sp., an unknown genus of Peptococcaceae and an unknown genus of Clostridiales in rats' cecum-and eight genes-including one downregulated Pla2g2a and seven upregulated genes: Apoa1, Apoa4, Aabr07073400.1, Fabp4, Pik3r5, Dgat2 and Ptgs2 of colonic epithelial cells-that were due to the consumption of catechin.
29748675	0	63	theme	cells	127:131	arg1	microbiota					74:83	cecal microbiota	68:83	cecal microbiota	68:83	Catechin supplemented in a FOS diet induces weight loss by altering cecal microbiota and gene expression of colonic epithelial cells.
29748675	0	63	theme	cells	127:131	arg1	expression					94:103	gene expression	89:103	gene expression	89:103	Catechin supplemented in a FOS diet induces weight loss by altering cecal microbiota and gene expression of colonic epithelial cells.
29748675	8	64	theme	colonic	1671:1677	arg1	cells					1690:1694	colonic epithelial cells	1671:1694	colonic epithelial cells	1671:1694	Consequently, various biological functions in connection with energy metabolism in colonic epithelial cells were altered, including fat digestion and absorption and the regulation of lipolysis in adipocytes.
29748675	1	65	theme	body	191:194	arg1	weights					196:202	rats' body weights	185:202	rats' body weights	185:202	Our previous study showed that catechin controlled rats' body weights and changed gut microbiota composition when supplemented into a high-fructo-oligosaccharide (FOS) diet.
29748675	7	66	theme	cells-that	1536:1545	arg1	Aabr07073400.1					1467:1480	Aabr07073400.1	1467:1480	Aabr07073400.1	1467:1480	and an unknown genus of YS2, and the decreased Lachnospira sp., Oscillospira sp., Ruminococcus sp., an unknown genus of Peptococcaceae and an unknown genus of Clostridiales in rats' cecum-and eight genes-including one downregulated Pla2g2a and seven upregulated genes: Apoa1, Apoa4, Aabr07073400.1, Fabp4, Pik3r5, Dgat2 and Ptgs2 of colonic epithelial cells-that were due to the consumption of catechin.
29748675	7	66	theme	cells-that	1536:1545	arg1	Dgat2					1498:1502	Dgat2	1498:1502	Dgat2	1498:1502	and an unknown genus of YS2, and the decreased Lachnospira sp., Oscillospira sp., Ruminococcus sp., an unknown genus of Peptococcaceae and an unknown genus of Clostridiales in rats' cecum-and eight genes-including one downregulated Pla2g2a and seven upregulated genes: Apoa1, Apoa4, Aabr07073400.1, Fabp4, Pik3r5, Dgat2 and Ptgs2 of colonic epithelial cells-that were due to the consumption of catechin.
29748675	7	66	theme	cells-that	1536:1545	arg1	Ptgs2					1508:1512	Ptgs2	1508:1512	Ptgs2	1508:1512	and an unknown genus of YS2, and the decreased Lachnospira sp., Oscillospira sp., Ruminococcus sp., an unknown genus of Peptococcaceae and an unknown genus of Clostridiales in rats' cecum-and eight genes-including one downregulated Pla2g2a and seven upregulated genes: Apoa1, Apoa4, Aabr07073400.1, Fabp4, Pik3r5, Dgat2 and Ptgs2 of colonic epithelial cells-that were due to the consumption of catechin.
29748675	7	66	theme	cells-that	1536:1545	arg1	Apoa1					1453:1457	Apoa1	1453:1457	Apoa1	1453:1457	and an unknown genus of YS2, and the decreased Lachnospira sp., Oscillospira sp., Ruminococcus sp., an unknown genus of Peptococcaceae and an unknown genus of Clostridiales in rats' cecum-and eight genes-including one downregulated Pla2g2a and seven upregulated genes: Apoa1, Apoa4, Aabr07073400.1, Fabp4, Pik3r5, Dgat2 and Ptgs2 of colonic epithelial cells-that were due to the consumption of catechin.
29748675	7	66	theme	cells-that	1536:1545	arg1	Fabp4					1483:1487	Fabp4	1483:1487	Fabp4	1483:1487	and an unknown genus of YS2, and the decreased Lachnospira sp., Oscillospira sp., Ruminococcus sp., an unknown genus of Peptococcaceae and an unknown genus of Clostridiales in rats' cecum-and eight genes-including one downregulated Pla2g2a and seven upregulated genes: Apoa1, Apoa4, Aabr07073400.1, Fabp4, Pik3r5, Dgat2 and Ptgs2 of colonic epithelial cells-that were due to the consumption of catechin.
29748675	7	66	theme	cells-that	1536:1545	arg1	Pik3r5					1490:1495	Pik3r5	1490:1495	Pik3r5	1490:1495	and an unknown genus of YS2, and the decreased Lachnospira sp., Oscillospira sp., Ruminococcus sp., an unknown genus of Peptococcaceae and an unknown genus of Clostridiales in rats' cecum-and eight genes-including one downregulated Pla2g2a and seven upregulated genes: Apoa1, Apoa4, Aabr07073400.1, Fabp4, Pik3r5, Dgat2 and Ptgs2 of colonic epithelial cells-that were due to the consumption of catechin.
29748675	7	66	theme	cells-that	1536:1545	arg1	Apoa4					1460:1464	Apoa4	1460:1464	Apoa4	1460:1464	and an unknown genus of YS2, and the decreased Lachnospira sp., Oscillospira sp., Ruminococcus sp., an unknown genus of Peptococcaceae and an unknown genus of Clostridiales in rats' cecum-and eight genes-including one downregulated Pla2g2a and seven upregulated genes: Apoa1, Apoa4, Aabr07073400.1, Fabp4, Pik3r5, Dgat2 and Ptgs2 of colonic epithelial cells-that were due to the consumption of catechin.
29748675	9	67	from	expression	1881:1890	arg1	cells					1927:1931	colonic epithelial cells	1908:1931	colonic epithelial cells	1908:1931	In conclusion, catechin induces host weight loss by altering gut microbiota and gene expression and function in colonic epithelial cells.
29748675	7	68	theme	colonic	1517:1523	arg1	cells-that					1536:1545	colonic epithelial cells-that	1517:1545	colonic epithelial cells-that	1517:1545	and an unknown genus of YS2, and the decreased Lachnospira sp., Oscillospira sp., Ruminococcus sp., an unknown genus of Peptococcaceae and an unknown genus of Clostridiales in rats' cecum-and eight genes-including one downregulated Pla2g2a and seven upregulated genes: Apoa1, Apoa4, Aabr07073400.1, Fabp4, Pik3r5, Dgat2 and Ptgs2 of colonic epithelial cells-that were due to the consumption of catechin.
29748675	7	69	theme	Peptococcaceae	1304:1317	arg1	Oscillospira					1248:1259	Oscillospira	1248:1259	Oscillospira	1248:1259	and an unknown genus of YS2, and the decreased Lachnospira sp., Oscillospira sp., Ruminococcus sp., an unknown genus of Peptococcaceae and an unknown genus of Clostridiales in rats' cecum-and eight genes-including one downregulated Pla2g2a and seven upregulated genes: Apoa1, Apoa4, Aabr07073400.1, Fabp4, Pik3r5, Dgat2 and Ptgs2 of colonic epithelial cells-that were due to the consumption of catechin.
29748675	7	69	theme	Peptococcaceae	1304:1317	arg1	genus					1295:1299	an unknown genus	1284:1299	an unknown genus of Peptococcaceae	1284:1317	and an unknown genus of YS2, and the decreased Lachnospira sp., Oscillospira sp., Ruminococcus sp., an unknown genus of Peptococcaceae and an unknown genus of Clostridiales in rats' cecum-and eight genes-including one downregulated Pla2g2a and seven upregulated genes: Apoa1, Apoa4, Aabr07073400.1, Fabp4, Pik3r5, Dgat2 and Ptgs2 of colonic epithelial cells-that were due to the consumption of catechin.
29748675	7	70	from	genus	1334:1338	arg1	rats					1360:1363	rats	1360:1363	rats	1360:1363	and an unknown genus of YS2, and the decreased Lachnospira sp., Oscillospira sp., Ruminococcus sp., an unknown genus of Peptococcaceae and an unknown genus of Clostridiales in rats' cecum-and eight genes-including one downregulated Pla2g2a and seven upregulated genes: Apoa1, Apoa4, Aabr07073400.1, Fabp4, Pik3r5, Dgat2 and Ptgs2 of colonic epithelial cells-that were due to the consumption of catechin.
29748675	9	71	theme	epithelial	1916:1925	arg1	cells					1927:1931	colonic epithelial cells	1908:1931	colonic epithelial cells	1908:1931	In conclusion, catechin induces host weight loss by altering gut microbiota and gene expression and function in colonic epithelial cells.
29748675	8	72	from	absorption	1738:1747	arg1	adipocytes					1784:1793	adipocytes	1784:1793	adipocytes	1784:1793	Consequently, various biological functions in connection with energy metabolism in colonic epithelial cells were altered, including fat digestion and absorption and the regulation of lipolysis in adipocytes.
29748675	7	73	from	Clostridiales	1343:1355	arg1	rats					1360:1363	rats	1360:1363	rats	1360:1363	and an unknown genus of YS2, and the decreased Lachnospira sp., Oscillospira sp., Ruminococcus sp., an unknown genus of Peptococcaceae and an unknown genus of Clostridiales in rats' cecum-and eight genes-including one downregulated Pla2g2a and seven upregulated genes: Apoa1, Apoa4, Aabr07073400.1, Fabp4, Pik3r5, Dgat2 and Ptgs2 of colonic epithelial cells-that were due to the consumption of catechin.
29748675	2	74	theme	body	480:483	arg1	weight					485:490	body weight	480:490	body weight	480:490	This experiment is devised to further confirm the relationship between specific bacteria in the colon and body weight gain, and to investigate how specific bacteria impact body weight by changing the expression of colonic epithelial cells.
29748675	3	75	theme	high-FOS	735:742	arg1	diet					744:747	a high-FOS diet	733:747	a high-FOS diet only	733:752	Forty obese rats were divided into four groups: three catechin-supplemented groups with a high-FOS diet (100, 400, and 700 mg kg-1 d-1 catechin, orally administered) and one group with a high-FOS diet only.
29748675	0	76	theme	cecal	68:72	arg1	microbiota					74:83	cecal microbiota	68:83	cecal microbiota	68:83	Catechin supplemented in a FOS diet induces weight loss by altering cecal microbiota and gene expression of colonic epithelial cells.
29748675	1	77	theme	previous	138:145	arg1	study					147:151	Our previous study	134:151	Our previous study	134:151	Our previous study showed that catechin controlled rats' body weights and changed gut microbiota composition when supplemented into a high-fructo-oligosaccharide (FOS) diet.
29748675	7	78	from	rats	1360:1363	arg1	genus					1334:1338	an unknown genus	1323:1338	an unknown genus of Clostridiales in rats	1323:1363	and an unknown genus of YS2, and the decreased Lachnospira sp., Oscillospira sp., Ruminococcus sp., an unknown genus of Peptococcaceae and an unknown genus of Clostridiales in rats' cecum-and eight genes-including one downregulated Pla2g2a and seven upregulated genes: Apoa1, Apoa4, Aabr07073400.1, Fabp4, Pik3r5, Dgat2 and Ptgs2 of colonic epithelial cells-that were due to the consumption of catechin.
29748675	6	79	theme	specific	1025:1032	arg1	bacteria					1034:1041	some specific bacteria	1020:1041	some specific bacteria	1020:1041	Results identified some specific bacteria at the genus level-including the increased Parabacteroides sp., Prevotella sp., Robinsoniella sp., [Ruminococcus], Phascolarctobacterium sp.
29748675	1	80	theme	high-fructo-oligosaccharide	268:294	arg1	diet					302:305	a high-fructo-oligosaccharide (FOS) diet	266:305	a high-fructo-oligosaccharide (FOS) diet	266:305	Our previous study showed that catechin controlled rats' body weights and changed gut microbiota composition when supplemented into a high-fructo-oligosaccharide (FOS) diet.
29748675	5	81	theme	colonic	868:874	arg1	cells					887:891	colonic epithelial cells	868:891	colonic epithelial cells	868:891	After one month of treatment, rats' cecal content and colonic epithelial cells were individually collected and analyzed with MiSeq and gene expression profiling techniques, respectively.
29748675	2	82	theme	weight	419:424	arg1	gain					426:429	body weight gain	414:429	body weight gain	414:429	This experiment is devised to further confirm the relationship between specific bacteria in the colon and body weight gain, and to investigate how specific bacteria impact body weight by changing the expression of colonic epithelial cells.
29748675	1	83	theme	FOS	297:299	arg1	diet					302:305	a high-fructo-oligosaccharide (FOS) diet	266:305	a high-fructo-oligosaccharide (FOS) diet	266:305	Our previous study showed that catechin controlled rats' body weights and changed gut microbiota composition when supplemented into a high-fructo-oligosaccharide (FOS) diet.
29748675	7	84	theme	catechin	1578:1585	arg1	consumption					1563:1573	the consumption	1559:1573	the consumption of catechin	1559:1585	and an unknown genus of YS2, and the decreased Lachnospira sp., Oscillospira sp., Ruminococcus sp., an unknown genus of Peptococcaceae and an unknown genus of Clostridiales in rats' cecum-and eight genes-including one downregulated Pla2g2a and seven upregulated genes: Apoa1, Apoa4, Aabr07073400.1, Fabp4, Pik3r5, Dgat2 and Ptgs2 of colonic epithelial cells-that were due to the consumption of catechin.
29748675	0	85	theme	weight	44:49	arg1	loss					51:54	weight loss	44:54	weight loss	44:54	Catechin supplemented in a FOS diet induces weight loss by altering cecal microbiota and gene expression of colonic epithelial cells.
29748675	5	86	theme	expression	954:963	arg1	techniques					975:984	gene expression profiling techniques	949:984	gene expression profiling techniques	949:984	After one month of treatment, rats' cecal content and colonic epithelial cells were individually collected and analyzed with MiSeq and gene expression profiling techniques, respectively.
29748675	6	87	theme	Parabacteroides	1086:1100	arg1	[Ruminococcus					1142:1154	the increased Parabacteroides sp., Prevotella sp., Robinsoniella sp., [Ruminococcus]	1072:1155	the increased Parabacteroides sp., Prevotella sp., Robinsoniella sp., [Ruminococcus]	1072:1155	Results identified some specific bacteria at the genus level-including the increased Parabacteroides sp., Prevotella sp., Robinsoniella sp., [Ruminococcus], Phascolarctobacterium sp.
29748675	6	87	theme	Parabacteroides	1086:1100	arg1	sp					1180:1181	Phascolarctobacterium sp	1158:1181	Phascolarctobacterium sp	1158:1181	Results identified some specific bacteria at the genus level-including the increased Parabacteroides sp., Prevotella sp., Robinsoniella sp., [Ruminococcus], Phascolarctobacterium sp.
29748675	7	88	dep	Lachnospira	1231:1241	arg1	sp.					1243:1245	Lachnospira sp.	1231:1245	Lachnospira sp.	1231:1245	and an unknown genus of YS2, and the decreased Lachnospira sp., Oscillospira sp., Ruminococcus sp., an unknown genus of Peptococcaceae and an unknown genus of Clostridiales in rats' cecum-and eight genes-including one downregulated Pla2g2a and seven upregulated genes: Apoa1, Apoa4, Aabr07073400.1, Fabp4, Pik3r5, Dgat2 and Ptgs2 of colonic epithelial cells-that were due to the consumption of catechin.
29748675	2	89	from	bacteria	388:395	arg1	colon					404:408	colon	404:408	colon	404:408	This experiment is devised to further confirm the relationship between specific bacteria in the colon and body weight gain, and to investigate how specific bacteria impact body weight by changing the expression of colonic epithelial cells.
29748675	2	89	from	bacteria	388:395	arg1	gain					426:429	body weight gain	414:429	body weight gain	414:429	This experiment is devised to further confirm the relationship between specific bacteria in the colon and body weight gain, and to investigate how specific bacteria impact body weight by changing the expression of colonic epithelial cells.
29748675	8	90	from	metabolism	1657:1666	arg1	cells					1690:1694	colonic epithelial cells	1671:1694	colonic epithelial cells	1671:1694	Consequently, various biological functions in connection with energy metabolism in colonic epithelial cells were altered, including fat digestion and absorption and the regulation of lipolysis in adipocytes.
29748675	8	91	theme	various	1602:1608	arg1	functions					1621:1629	various biological functions	1602:1629	various biological functions in connection with energy metabolism in colonic epithelial cells	1602:1694	Consequently, various biological functions in connection with energy metabolism in colonic epithelial cells were altered, including fat digestion and absorption and the regulation of lipolysis in adipocytes.
29748675	1	92	theme	microbiota	220:229	arg1	composition					231:241	gut microbiota composition	216:241	gut microbiota composition	216:241	Our previous study showed that catechin controlled rats' body weights and changed gut microbiota composition when supplemented into a high-fructo-oligosaccharide (FOS) diet.
30581114	5	0	theme	short-chain	924:934	arg1	acids					942:946	short-chain fatty acids	924:946	short-chain fatty acids	924:946	However, RS suppressed the abundance and translocation of L. reuteri via short-chain fatty acids, which inhibited its growth.
30581114	1	1	from	changes	171:177	arg1	gut					191:193	gut	191:193	gut	191:193	Western lifestyle is linked to autoimmune and metabolic diseases, driven by changes in diet and gut microbiota composition.
30581114	1	1	from	changes	171:177	arg1	diet					182:185	diet	182:185	diet	182:185	Western lifestyle is linked to autoimmune and metabolic diseases, driven by changes in diet and gut microbiota composition.
30581114	3	2	theme	internal	487:494	arg1	organs					496:501	internal organs	487:501	internal organs	487:501	Culture of internal organs and 16S rDNA sequencing revealed TLR7-dependent translocation of L. reuteri in mice and fecal enrichment of Lactobacillus in a subset of SLE patients.
30581114	4	3	dep	L.	654:655	arg1	reuteri					657:663	reuteri	657:663	reuteri	657:663	L. reuteri colonization worsened autoimmune manifestations under specific-pathogen-free and gnotobiotic conditions, notably increasing plasmacytoid dendritic cells (pDCs) and interferon signaling.
30581114	2	4	theme	erythematosus	294:306	arg1	models					269:274	Toll-like receptor 7 (TLR7)-dependent mouse models	225:274	Toll-like receptor 7 (TLR7)-dependent mouse models of systemic lupus erythematosus (SLE)	225:312	Using Toll-like receptor 7 (TLR7)-dependent mouse models of systemic lupus erythematosus (SLE), we dissect dietary effects on the gut microbiota and find that Lactobacillus reuteri can drive autoimmunity but is ameliorated by dietary resistant starch (RS).
30581114	6	5	theme	interferon	1010:1019	arg1	pathways					1021:1028	interferon pathways	1010:1028	interferon pathways	1010:1028	Additionally, RS decreased pDCs, interferon pathways, organ involvement, and mortality.
30581114	6	5	theme	interferon	1010:1019	arg1	pDCs					1004:1007	pDCs	1004:1007	pDCs	1004:1007	Additionally, RS decreased pDCs, interferon pathways, organ involvement, and mortality.
30581114	4	6	theme	plasmacytoid	789:800	arg1	pDCs					819:822	pDCs	819:822	pDCs	819:822	L. reuteri colonization worsened autoimmune manifestations under specific-pathogen-free and gnotobiotic conditions, notably increasing plasmacytoid dendritic cells (pDCs) and interferon signaling.
30581114	4	6	theme	plasmacytoid	789:800	arg1	cells					812:816	plasmacytoid dendritic cells	789:816	plasmacytoid dendritic cells (pDCs)	789:823	L. reuteri colonization worsened autoimmune manifestations under specific-pathogen-free and gnotobiotic conditions, notably increasing plasmacytoid dendritic cells (pDCs) and interferon signaling.
30581114	2	7	from	effects	334:340	arg1	microbiota					353:362	the gut microbiota	345:362	the gut microbiota	345:362	Using Toll-like receptor 7 (TLR7)-dependent mouse models of systemic lupus erythematosus (SLE), we dissect dietary effects on the gut microbiota and find that Lactobacillus reuteri can drive autoimmunity but is ameliorated by dietary resistant starch (RS).
30581114	2	8	theme	lupus	288:292	arg1	SLE					309:311	SLE	309:311	SLE	309:311	Using Toll-like receptor 7 (TLR7)-dependent mouse models of systemic lupus erythematosus (SLE), we dissect dietary effects on the gut microbiota and find that Lactobacillus reuteri can drive autoimmunity but is ameliorated by dietary resistant starch (RS).
30581114	2	8	theme	lupus	288:292	arg1	erythematosus					294:306	systemic lupus erythematosus	279:306	systemic lupus erythematosus (SLE)	279:312	Using Toll-like receptor 7 (TLR7)-dependent mouse models of systemic lupus erythematosus (SLE), we dissect dietary effects on the gut microbiota and find that Lactobacillus reuteri can drive autoimmunity but is ameliorated by dietary resistant starch (RS).
30581114	3	9	from	enrichment	597:606	arg1	mice					582:585	mice	582:585	mice	582:585	Culture of internal organs and 16S rDNA sequencing revealed TLR7-dependent translocation of L. reuteri in mice and fecal enrichment of Lactobacillus in a subset of SLE patients.
30581114	4	10	theme	autoimmune	687:696	arg1	manifestations					698:711	autoimmune manifestations	687:711	autoimmune manifestations	687:711	L. reuteri colonization worsened autoimmune manifestations under specific-pathogen-free and gnotobiotic conditions, notably increasing plasmacytoid dendritic cells (pDCs) and interferon signaling.
30581114	1	11	theme	Western	95:101	arg1	lifestyle					103:111	Western lifestyle	95:111	Western lifestyle	95:111	Western lifestyle is linked to autoimmune and metabolic diseases, driven by changes in diet and gut microbiota composition.
30581114	2	12	dep	-dependent	252:261	arg1	receptor					235:242	Toll-like receptor 7	225:244	Toll-like receptor 7	225:244	Using Toll-like receptor 7 (TLR7)-dependent mouse models of systemic lupus erythematosus (SLE), we dissect dietary effects on the gut microbiota and find that Lactobacillus reuteri can drive autoimmunity but is ameliorated by dietary resistant starch (RS).
30581114	2	13	theme	dietary	445:451	arg1	RS					471:472	RS	471:472	RS	471:472	Using Toll-like receptor 7 (TLR7)-dependent mouse models of systemic lupus erythematosus (SLE), we dissect dietary effects on the gut microbiota and find that Lactobacillus reuteri can drive autoimmunity but is ameliorated by dietary resistant starch (RS).
30581114	2	13	theme	dietary	445:451	arg1	starch					463:468	dietary resistant starch	445:468	dietary resistant starch (RS)	445:473	Using Toll-like receptor 7 (TLR7)-dependent mouse models of systemic lupus erythematosus (SLE), we dissect dietary effects on the gut microbiota and find that Lactobacillus reuteri can drive autoimmunity but is ameliorated by dietary resistant starch (RS).
30581114	5	14	theme	fatty	936:940	arg1	acids					942:946	short-chain fatty acids	924:946	short-chain fatty acids	924:946	However, RS suppressed the abundance and translocation of L. reuteri via short-chain fatty acids, which inhibited its growth.
30581114	3	15	theme	rDNA	511:514	arg1	sequencing					516:525	16S rDNA sequencing	507:525	16S rDNA sequencing	507:525	Culture of internal organs and 16S rDNA sequencing revealed TLR7-dependent translocation of L. reuteri in mice and fecal enrichment of Lactobacillus in a subset of SLE patients.
30581114	0	16	theme	Diet-Sensitive	2:15	arg1	Strain					41:46	A Diet-Sensitive Commensal Lactobacillus Strain	0:46	A Diet-Sensitive Commensal Lactobacillus Strain	0:46	A Diet-Sensitive Commensal Lactobacillus Strain Mediates TLR7-Dependent Systemic Autoimmunity.
30581114	5	17	dep	abundance	878:886	arg1	the					874:876	the	874:876	the	874:876	However, RS suppressed the abundance and translocation of L. reuteri via short-chain fatty acids, which inhibited its growth.
30581114	7	18	theme	lupus-prone	1103:1113	arg1	hosts					1115:1119	lupus-prone hosts	1103:1119	lupus-prone hosts	1103:1119	Thus, RS exerts beneficial effects in lupus-prone hosts through suppressing a pathobiont that promotes interferon pathways implicated in the pathogenesis of human autoimmunity.
30581114	3	19	theme	16S	507:509	arg1	sequencing					516:525	16S rDNA sequencing	507:525	16S rDNA sequencing	507:525	Culture of internal organs and 16S rDNA sequencing revealed TLR7-dependent translocation of L. reuteri in mice and fecal enrichment of Lactobacillus in a subset of SLE patients.
30581114	7	20	theme	autoimmunity	1228:1239	arg1	the pathogenesis					1202:1217	the pathogenesis	1202:1217	the pathogenesis of human autoimmunity	1202:1239	Thus, RS exerts beneficial effects in lupus-prone hosts through suppressing a pathobiont that promotes interferon pathways implicated in the pathogenesis of human autoimmunity.
30581114	3	21	theme	L. reuteri	568:577	arg1	translocation					551:563	TLR7-dependent translocation	536:563	TLR7-dependent translocation of L. reuteri in mice	536:585	Culture of internal organs and 16S rDNA sequencing revealed TLR7-dependent translocation of L. reuteri in mice and fecal enrichment of Lactobacillus in a subset of SLE patients.
30581114	3	21	theme	L. reuteri	568:577	arg1	enrichment					597:606	fecal enrichment	591:606	fecal enrichment of Lactobacillus	591:623	Culture of internal organs and 16S rDNA sequencing revealed TLR7-dependent translocation of L. reuteri in mice and fecal enrichment of Lactobacillus in a subset of SLE patients.
30581114	4	22	theme	dendritic	802:810	arg1	pDCs					819:822	pDCs	819:822	pDCs	819:822	L. reuteri colonization worsened autoimmune manifestations under specific-pathogen-free and gnotobiotic conditions, notably increasing plasmacytoid dendritic cells (pDCs) and interferon signaling.
30581114	4	22	theme	dendritic	802:810	arg1	cells					812:816	plasmacytoid dendritic cells	789:816	plasmacytoid dendritic cells (pDCs)	789:823	L. reuteri colonization worsened autoimmune manifestations under specific-pathogen-free and gnotobiotic conditions, notably increasing plasmacytoid dendritic cells (pDCs) and interferon signaling.
30581114	1	23	theme	microbiota	195:204	arg1	composition					206:216	microbiota composition	195:216	microbiota composition	195:216	Western lifestyle is linked to autoimmune and metabolic diseases, driven by changes in diet and gut microbiota composition.
30581114	0	24	theme	Lactobacillus	27:39	arg1	Strain					41:46	A Diet-Sensitive Commensal Lactobacillus Strain	0:46	A Diet-Sensitive Commensal Lactobacillus Strain	0:46	A Diet-Sensitive Commensal Lactobacillus Strain Mediates TLR7-Dependent Systemic Autoimmunity.
30581114	4	25	theme	interferon	829:838	arg1	signaling					840:848	interferon signaling	829:848	interferon signaling	829:848	L. reuteri colonization worsened autoimmune manifestations under specific-pathogen-free and gnotobiotic conditions, notably increasing plasmacytoid dendritic cells (pDCs) and interferon signaling.
30581114	3	26	theme	TLR7-dependent	536:549	arg1	translocation					551:563	TLR7-dependent translocation	536:563	TLR7-dependent translocation of L. reuteri in mice	536:585	Culture of internal organs and 16S rDNA sequencing revealed TLR7-dependent translocation of L. reuteri in mice and fecal enrichment of Lactobacillus in a subset of SLE patients.
30581114	0	27	theme	Commensal	17:25	arg1	Strain					41:46	A Diet-Sensitive Commensal Lactobacillus Strain	0:46	A Diet-Sensitive Commensal Lactobacillus Strain	0:46	A Diet-Sensitive Commensal Lactobacillus Strain Mediates TLR7-Dependent Systemic Autoimmunity.
30581114	2	28	theme	systemic	279:286	arg1	SLE					309:311	SLE	309:311	SLE	309:311	Using Toll-like receptor 7 (TLR7)-dependent mouse models of systemic lupus erythematosus (SLE), we dissect dietary effects on the gut microbiota and find that Lactobacillus reuteri can drive autoimmunity but is ameliorated by dietary resistant starch (RS).
30581114	2	28	theme	systemic	279:286	arg1	erythematosus					294:306	systemic lupus erythematosus	279:306	systemic lupus erythematosus (SLE)	279:312	Using Toll-like receptor 7 (TLR7)-dependent mouse models of systemic lupus erythematosus (SLE), we dissect dietary effects on the gut microbiota and find that Lactobacillus reuteri can drive autoimmunity but is ameliorated by dietary resistant starch (RS).
30581114	1	29	theme	autoimmune	126:135	arg1	diseases					151:158	autoimmune and metabolic diseases	126:158	autoimmune and metabolic diseases	126:158	Western lifestyle is linked to autoimmune and metabolic diseases, driven by changes in diet and gut microbiota composition.
30581114	5	30	theme	reuteri	912:918	arg1	translocation					892:904	translocation	892:904	translocation	892:904	However, RS suppressed the abundance and translocation of L. reuteri via short-chain fatty acids, which inhibited its growth.
30581114	5	30	theme	reuteri	912:918	arg1	abundance					878:886	abundance	878:886	abundance	878:886	However, RS suppressed the abundance and translocation of L. reuteri via short-chain fatty acids, which inhibited its growth.
30581114	2	31	theme	dietary	326:332	arg1	effects					334:340	dietary effects	326:340	dietary effects on the gut microbiota	326:362	Using Toll-like receptor 7 (TLR7)-dependent mouse models of systemic lupus erythematosus (SLE), we dissect dietary effects on the gut microbiota and find that Lactobacillus reuteri can drive autoimmunity but is ameliorated by dietary resistant starch (RS).
30581114	3	32	theme	SLE	640:642	arg1	patients					644:651	SLE patients	640:651	SLE patients	640:651	Culture of internal organs and 16S rDNA sequencing revealed TLR7-dependent translocation of L. reuteri in mice and fecal enrichment of Lactobacillus in a subset of SLE patients.
30581114	4	33	theme	L.	654:655	arg1	colonization					665:676	L. reuteri colonization	654:676	L. reuteri colonization	654:676	L. reuteri colonization worsened autoimmune manifestations under specific-pathogen-free and gnotobiotic conditions, notably increasing plasmacytoid dendritic cells (pDCs) and interferon signaling.
30581114	7	34	theme	interferon	1168:1177	arg1	pathways					1179:1186	interferon pathways	1168:1186	interferon pathways implicated in the pathogenesis of human autoimmunity	1168:1239	Thus, RS exerts beneficial effects in lupus-prone hosts through suppressing a pathobiont that promotes interferon pathways implicated in the pathogenesis of human autoimmunity.
30581114	1	35	attach	linked	116:121	arg1	diseases					151:158	autoimmune and metabolic diseases	126:158	autoimmune and metabolic diseases	126:158	Western lifestyle is linked to autoimmune and metabolic diseases, driven by changes in diet and gut microbiota composition.
30581114	1	35	attach	linked	116:121	arg2	lifestyle					103:111	Western lifestyle	95:111	Western lifestyle	95:111	Western lifestyle is linked to autoimmune and metabolic diseases, driven by changes in diet and gut microbiota composition.
30581114	7	36	theme	beneficial	1081:1090	arg1	effects					1092:1098	beneficial effects	1081:1098	beneficial effects	1081:1098	Thus, RS exerts beneficial effects in lupus-prone hosts through suppressing a pathobiont that promotes interferon pathways implicated in the pathogenesis of human autoimmunity.
30581114	3	37	theme	fecal	591:595	arg1	enrichment					597:606	fecal enrichment	591:606	fecal enrichment of Lactobacillus	591:623	Culture of internal organs and 16S rDNA sequencing revealed TLR7-dependent translocation of L. reuteri in mice and fecal enrichment of Lactobacillus in a subset of SLE patients.
30581114	2	38	theme	resistant	453:461	arg1	RS					471:472	RS	471:472	RS	471:472	Using Toll-like receptor 7 (TLR7)-dependent mouse models of systemic lupus erythematosus (SLE), we dissect dietary effects on the gut microbiota and find that Lactobacillus reuteri can drive autoimmunity but is ameliorated by dietary resistant starch (RS).
30581114	2	38	theme	resistant	453:461	arg1	starch					463:468	dietary resistant starch	445:468	dietary resistant starch (RS)	445:473	Using Toll-like receptor 7 (TLR7)-dependent mouse models of systemic lupus erythematosus (SLE), we dissect dietary effects on the gut microbiota and find that Lactobacillus reuteri can drive autoimmunity but is ameliorated by dietary resistant starch (RS).
30581114	1	39	dep	diet	182:185	arg1	composition					206:216	microbiota composition	195:216	microbiota composition	195:216	Western lifestyle is linked to autoimmune and metabolic diseases, driven by changes in diet and gut microbiota composition.
30581114	4	40	theme	specific-pathogen-free	719:740	arg1	conditions					758:767	specific-pathogen-free and gnotobiotic conditions	719:767	specific-pathogen-free and gnotobiotic conditions	719:767	L. reuteri colonization worsened autoimmune manifestations under specific-pathogen-free and gnotobiotic conditions, notably increasing plasmacytoid dendritic cells (pDCs) and interferon signaling.
30581114	0	41	theme	TLR7-Dependent	57:70	arg1	Autoimmunity					81:92	TLR7-Dependent Systemic Autoimmunity	57:92	TLR7-Dependent Systemic Autoimmunity	57:92	A Diet-Sensitive Commensal Lactobacillus Strain Mediates TLR7-Dependent Systemic Autoimmunity.
30581114	2	42	theme	Toll-like	225:233	arg1	receptor					235:242	Toll-like receptor 7	225:244	Toll-like receptor 7	225:244	Using Toll-like receptor 7 (TLR7)-dependent mouse models of systemic lupus erythematosus (SLE), we dissect dietary effects on the gut microbiota and find that Lactobacillus reuteri can drive autoimmunity but is ameliorated by dietary resistant starch (RS).
30581114	1	43	theme	metabolic	141:149	arg1	diseases					151:158	autoimmune and metabolic diseases	126:158	autoimmune and metabolic diseases	126:158	Western lifestyle is linked to autoimmune and metabolic diseases, driven by changes in diet and gut microbiota composition.
30581114	7	44	theme	human	1222:1226	arg1	autoimmunity					1228:1239	human autoimmunity	1222:1239	human autoimmunity	1222:1239	Thus, RS exerts beneficial effects in lupus-prone hosts through suppressing a pathobiont that promotes interferon pathways implicated in the pathogenesis of human autoimmunity.
30581114	3	45	theme	Lactobacillus	611:623	arg1	translocation					551:563	TLR7-dependent translocation	536:563	TLR7-dependent translocation of L. reuteri in mice	536:585	Culture of internal organs and 16S rDNA sequencing revealed TLR7-dependent translocation of L. reuteri in mice and fecal enrichment of Lactobacillus in a subset of SLE patients.
30581114	3	45	theme	Lactobacillus	611:623	arg1	enrichment					597:606	fecal enrichment	591:606	fecal enrichment of Lactobacillus	591:623	Culture of internal organs and 16S rDNA sequencing revealed TLR7-dependent translocation of L. reuteri in mice and fecal enrichment of Lactobacillus in a subset of SLE patients.
30581114	4	46	theme	gnotobiotic	746:756	arg1	conditions					758:767	specific-pathogen-free and gnotobiotic conditions	719:767	specific-pathogen-free and gnotobiotic conditions	719:767	L. reuteri colonization worsened autoimmune manifestations under specific-pathogen-free and gnotobiotic conditions, notably increasing plasmacytoid dendritic cells (pDCs) and interferon signaling.
30581114	3	47	from	translocation	551:563	arg1	mice					582:585	mice	582:585	mice	582:585	Culture of internal organs and 16S rDNA sequencing revealed TLR7-dependent translocation of L. reuteri in mice and fecal enrichment of Lactobacillus in a subset of SLE patients.
30581114	3	48	theme	patients	644:651	arg1	subset					630:635	a subset	628:635	a subset of SLE patients	628:651	Culture of internal organs and 16S rDNA sequencing revealed TLR7-dependent translocation of L. reuteri in mice and fecal enrichment of Lactobacillus in a subset of SLE patients.
30581114	2	49	theme	mouse	263:267	arg1	models					269:274	Toll-like receptor 7 (TLR7)-dependent mouse models	225:274	Toll-like receptor 7 (TLR7)-dependent mouse models of systemic lupus erythematosus (SLE)	225:312	Using Toll-like receptor 7 (TLR7)-dependent mouse models of systemic lupus erythematosus (SLE), we dissect dietary effects on the gut microbiota and find that Lactobacillus reuteri can drive autoimmunity but is ameliorated by dietary resistant starch (RS).
30581114	2	50	theme	-dependent	252:261	arg1	models					269:274	Toll-like receptor 7 (TLR7)-dependent mouse models	225:274	Toll-like receptor 7 (TLR7)-dependent mouse models of systemic lupus erythematosus (SLE)	225:312	Using Toll-like receptor 7 (TLR7)-dependent mouse models of systemic lupus erythematosus (SLE), we dissect dietary effects on the gut microbiota and find that Lactobacillus reuteri can drive autoimmunity but is ameliorated by dietary resistant starch (RS).
30581114	3	51	theme	organs	496:501	arg1	Culture					476:482	Culture	476:482	Culture of internal organs and 16S rDNA sequencing	476:525	Culture of internal organs and 16S rDNA sequencing revealed TLR7-dependent translocation of L. reuteri in mice and fecal enrichment of Lactobacillus in a subset of SLE patients.
30581114	0	52	theme	Systemic	72:79	arg1	Autoimmunity					81:92	TLR7-Dependent Systemic Autoimmunity	57:92	TLR7-Dependent Systemic Autoimmunity	57:92	A Diet-Sensitive Commensal Lactobacillus Strain Mediates TLR7-Dependent Systemic Autoimmunity.
30581114	3	53	theme	sequencing	516:525	arg1	Culture					476:482	Culture	476:482	Culture of internal organs and 16S rDNA sequencing	476:525	Culture of internal organs and 16S rDNA sequencing revealed TLR7-dependent translocation of L. reuteri in mice and fecal enrichment of Lactobacillus in a subset of SLE patients.
30581114	2	54	theme	gut	349:351	arg1	microbiota					353:362	the gut microbiota	345:362	the gut microbiota	345:362	Using Toll-like receptor 7 (TLR7)-dependent mouse models of systemic lupus erythematosus (SLE), we dissect dietary effects on the gut microbiota and find that Lactobacillus reuteri can drive autoimmunity but is ameliorated by dietary resistant starch (RS).
30581114	6	55	theme	organ	1031:1035	arg1	involvement					1037:1047	organ involvement	1031:1047	organ involvement	1031:1047	Additionally, RS decreased pDCs, interferon pathways, organ involvement, and mortality.
30581114	6	55	theme	organ	1031:1035	arg1	pDCs					1004:1007	pDCs	1004:1007	pDCs	1004:1007	Additionally, RS decreased pDCs, interferon pathways, organ involvement, and mortality.
29491059	0	0	theme	Expression	115:124	arg1	Down-regulation					78:92	Hypoxia-induced Down-regulation	62:92	Hypoxia-induced Down-regulation of Cell-surface MICA Expression	62:124	Inhibition of Asparagine-linked Glycosylation Participates in Hypoxia-induced Down-regulation of Cell-surface MICA Expression.
29491059	8	1	theme	Asn-N-linked	1300:1311	arg1	glycosylation					1313:1325	Asn-N-linked glycosylation	1300:1325	Asn-N-linked glycosylation	1300:1325	CONCLUSION The inhibition of Asn-N-linked glycosylation participates in hypoxia-induced down-regulation of cell-surface expression of MICA.
29491059	7	2	theme	expression	1110:1119	arg1	vector					1121:1126	wild-type MICA expression vector	1095:1126	wild-type MICA expression vector	1095:1126	Cells transfected with wild-type MICA expression vector expressed cell surface MICA more than those transfected with mutant MICA expression vectors designed for abrogation of N-linked glycosylation.
29491059	1	3	theme	histocompatibility	202:219	arg1	class					221:225	major histocompatibility class	196:225	cell surface major histocompatibility class I-related chain molecule A (MICA)	183:259	BACKGROUND/AIM Hypoxia down-regulates the expression of cell surface major histocompatibility class I-related chain molecule A (MICA) without increasing its shedding.
29491059	0	4	theme	MICA	110:113	arg1	Expression					115:124	Cell-surface MICA Expression	97:124	Cell-surface MICA Expression	97:124	Inhibition of Asparagine-linked Glycosylation Participates in Hypoxia-induced Down-regulation of Cell-surface MICA Expression.
29491059	4	5	theme	cell-surface	590:601	arg1	expression					603:612	The cell-surface expression	586:612	The cell-surface expression	586:612	MATERIALS AND METHODS The cell-surface expression and Asn-N-glycosylation of MICA were estimated by flow cytometry, and western blot analyses, respectively.
29491059	4	5	theme	cell-surface	590:601	arg1	MATERIALS					564:572	MATERIALS	564:572	MATERIALS	564:572	MATERIALS AND METHODS The cell-surface expression and Asn-N-glycosylation of MICA were estimated by flow cytometry, and western blot analyses, respectively.
29491059	2	6	theme	MICA	401:404	arg1	expression					387:396	the cell-surface expression	370:396	the cell-surface expression of MICA	370:404	Recently, the inhibition of N-linked glycosylation was also shown to reduce the cell-surface expression of MICA.
29491059	7	7	theme	wild-type	1095:1103	arg1	vector					1121:1126	wild-type MICA expression vector	1095:1126	wild-type MICA expression vector	1095:1126	Cells transfected with wild-type MICA expression vector expressed cell surface MICA more than those transfected with mutant MICA expression vectors designed for abrogation of N-linked glycosylation.
29491059	4	8	theme	flow	664:667	arg1	cytometry					669:677	flow cytometry	664:677	flow cytometry	664:677	MATERIALS AND METHODS The cell-surface expression and Asn-N-glycosylation of MICA were estimated by flow cytometry, and western blot analyses, respectively.
29491059	7	9	theme	N-linked	1247:1254	arg1	glycosylation					1256:1268	N-linked glycosylation	1247:1268	N-linked glycosylation	1247:1268	Cells transfected with wild-type MICA expression vector expressed cell surface MICA more than those transfected with mutant MICA expression vectors designed for abrogation of N-linked glycosylation.
29491059	0	10	link	Asparagine-linked	14:30	arg1	Glycosylation					32:44	Asparagine-linked Glycosylation	14:44	Asparagine-linked Glycosylation	14:44	Inhibition of Asparagine-linked Glycosylation Participates in Hypoxia-induced Down-regulation of Cell-surface MICA Expression.
29491059	5	11	gly	non-glycosylated	840:855	arg1	MICA					857:860	non-glycosylated MICA	840:860	non-glycosylated MICA	840:860	RESULTS Hypoxia reduced the expression of N-linked glycosylated MICA, as well as the ratio of N-linked glycosylated to non-glycosylated MICA.
29491059	8	12	theme	glycosylation	1313:1325	arg1	inhibition					1286:1295	The inhibition	1282:1295	The inhibition of Asn-N-linked glycosylation	1282:1325	CONCLUSION The inhibition of Asn-N-linked glycosylation participates in hypoxia-induced down-regulation of cell-surface expression of MICA.
29491059	6	13	theme	N-glycosylated	1007:1020	arg1	MICA					1022:1025	N-glycosylated MICA	1007:1025	N-glycosylated MICA	1007:1025	2-Deoxy-D-glucose, which inhibits N-linked glycosylation, reduced the cell-surface expression of MICA under normoxia, while D-Mannose increased N-glycosylated MICA, increasing cell-surface MICA under hypoxia.
29491059	7	14	theme	glycosylation	1256:1268	arg1	abrogation					1233:1242	abrogation	1233:1242	abrogation of N-linked glycosylation	1233:1268	Cells transfected with wild-type MICA expression vector expressed cell surface MICA more than those transfected with mutant MICA expression vectors designed for abrogation of N-linked glycosylation.
29491059	2	15	theme	glycosylation	331:343	arg1	inhibition					308:317	the inhibition	304:317	the inhibition of N-linked glycosylation	304:343	Recently, the inhibition of N-linked glycosylation was also shown to reduce the cell-surface expression of MICA.
29491059	5	16	theme	N-linked	815:822	arg1	ratio					806:810	the ratio	802:810	the ratio of N-linked	802:822	RESULTS Hypoxia reduced the expression of N-linked glycosylated MICA, as well as the ratio of N-linked glycosylated to non-glycosylated MICA.
29491059	5	16	theme	N-linked	815:822	arg1	expression					749:758	the expression	745:758	the expression of N-linked glycosylated MICA	745:788	RESULTS Hypoxia reduced the expression of N-linked glycosylated MICA, as well as the ratio of N-linked glycosylated to non-glycosylated MICA.
29491059	3	17	from	participation	427:439	arg1	down-regulation					501:515	hypoxia-induced down-regulation	485:515	hypoxia-induced down-regulation of cell-surface MICA	485:536	We investigated the participation of asparagine (Asn)-linked glycosylation in hypoxia-induced down-regulation of cell-surface MICA using osteosarcoma cells.
29491059	8	18	theme	hypoxia-induced	1343:1357	arg1	down-regulation					1359:1373	hypoxia-induced down-regulation	1343:1373	hypoxia-induced down-regulation of cell-surface expression of MICA	1343:1408	CONCLUSION The inhibition of Asn-N-linked glycosylation participates in hypoxia-induced down-regulation of cell-surface expression of MICA.
29491059	6	19	theme	N-linked	897:904	arg1	glycosylation					906:918	N-linked glycosylation	897:918	N-linked glycosylation	897:918	2-Deoxy-D-glucose, which inhibits N-linked glycosylation, reduced the cell-surface expression of MICA under normoxia, while D-Mannose increased N-glycosylated MICA, increasing cell-surface MICA under hypoxia.
29491059	2	20	theme	N-linked	322:329	arg1	glycosylation					331:343	N-linked glycosylation	322:343	N-linked glycosylation	322:343	Recently, the inhibition of N-linked glycosylation was also shown to reduce the cell-surface expression of MICA.
29491059	3	21	theme	glycosylation	468:480	arg1	participation					427:439	the participation	423:439	the participation of asparagine (Asn)-linked glycosylation in hypoxia-induced down-regulation of cell-surface MICA	423:536	We investigated the participation of asparagine (Asn)-linked glycosylation in hypoxia-induced down-regulation of cell-surface MICA using osteosarcoma cells.
29491059	1	22	theme	I-related	227:235	arg1	MICA					255:258	MICA	255:258	MICA	255:258	BACKGROUND/AIM Hypoxia down-regulates the expression of cell surface major histocompatibility class I-related chain molecule A (MICA) without increasing its shedding.
29491059	1	22	theme	I-related	227:235	arg1	A					252:252	major histocompatibility class I-related chain molecule A	196:252	cell surface major histocompatibility class I-related chain molecule A (MICA)	183:259	BACKGROUND/AIM Hypoxia down-regulates the expression of cell surface major histocompatibility class I-related chain molecule A (MICA) without increasing its shedding.
29491059	6	23	theme	MICA	960:963	arg1	expression					946:955	the cell-surface expression	929:955	the cell-surface expression of MICA	929:963	2-Deoxy-D-glucose, which inhibits N-linked glycosylation, reduced the cell-surface expression of MICA under normoxia, while D-Mannose increased N-glycosylated MICA, increasing cell-surface MICA under hypoxia.
29491059	4	24	theme	western	684:690	arg1	analyses					697:704	western blot analyses	684:704	western blot analyses	684:704	MATERIALS AND METHODS The cell-surface expression and Asn-N-glycosylation of MICA were estimated by flow cytometry, and western blot analyses, respectively.
29491059	1	25	theme	chain	237:241	arg1	MICA					255:258	MICA	255:258	MICA	255:258	BACKGROUND/AIM Hypoxia down-regulates the expression of cell surface major histocompatibility class I-related chain molecule A (MICA) without increasing its shedding.
29491059	1	25	theme	chain	237:241	arg1	A					252:252	major histocompatibility class I-related chain molecule A	196:252	cell surface major histocompatibility class I-related chain molecule A (MICA)	183:259	BACKGROUND/AIM Hypoxia down-regulates the expression of cell surface major histocompatibility class I-related chain molecule A (MICA) without increasing its shedding.
29491059	8	26	dep	CONCLUSION	1271:1280	arg1	participates					1327:1338	participates	1327:1338	participates in hypoxia-induced down-regulation of cell-surface expression of MICA	1327:1408	CONCLUSION The inhibition of Asn-N-linked glycosylation participates in hypoxia-induced down-regulation of cell-surface expression of MICA.
29491059	5	27	theme	RESULTS	721:727	arg1	Hypoxia					729:735	RESULTS Hypoxia	721:735	RESULTS Hypoxia	721:735	RESULTS Hypoxia reduced the expression of N-linked glycosylated MICA, as well as the ratio of N-linked glycosylated to non-glycosylated MICA.
29491059	4	28	dep	MATERIALS	564:572	arg1	Asn-N-glycosylation					618:636	Asn-N-glycosylation	618:636	Asn-N-glycosylation of MICA	618:644	MATERIALS AND METHODS The cell-surface expression and Asn-N-glycosylation of MICA were estimated by flow cytometry, and western blot analyses, respectively.
29491059	4	28	dep	MATERIALS	564:572	arg1	expression					603:612	The cell-surface expression	586:612	The cell-surface expression	586:612	MATERIALS AND METHODS The cell-surface expression and Asn-N-glycosylation of MICA were estimated by flow cytometry, and western blot analyses, respectively.
29491059	4	28	dep	MATERIALS	564:572	arg1	MATERIALS					564:572	MATERIALS	564:572	MATERIALS	564:572	MATERIALS AND METHODS The cell-surface expression and Asn-N-glycosylation of MICA were estimated by flow cytometry, and western blot analyses, respectively.
29491059	1	29	theme	molecule	243:250	arg1	MICA					255:258	MICA	255:258	MICA	255:258	BACKGROUND/AIM Hypoxia down-regulates the expression of cell surface major histocompatibility class I-related chain molecule A (MICA) without increasing its shedding.
29491059	1	29	theme	molecule	243:250	arg1	A					252:252	major histocompatibility class I-related chain molecule A	196:252	cell surface major histocompatibility class I-related chain molecule A (MICA)	183:259	BACKGROUND/AIM Hypoxia down-regulates the expression of cell surface major histocompatibility class I-related chain molecule A (MICA) without increasing its shedding.
29491059	2	30	link	N-linked	322:329	arg1	glycosylation					331:343	N-linked glycosylation	322:343	N-linked glycosylation	322:343	Recently, the inhibition of N-linked glycosylation was also shown to reduce the cell-surface expression of MICA.
29491059	1	31	theme	BACKGROUND/AIM	127:140	arg1	Hypoxia					142:148	BACKGROUND/AIM Hypoxia	127:148	BACKGROUND/AIM Hypoxia	127:148	BACKGROUND/AIM Hypoxia down-regulates the expression of cell surface major histocompatibility class I-related chain molecule A (MICA) without increasing its shedding.
29491059	0	32	theme	Glycosylation	32:44	arg1	Inhibition					0:9	Inhibition	0:9	Inhibition of Asparagine-linked Glycosylation	0:44	Inhibition of Asparagine-linked Glycosylation Participates in Hypoxia-induced Down-regulation of Cell-surface MICA Expression.
29491059	4	33	theme	MICA	641:644	arg1	Asn-N-glycosylation					618:636	Asn-N-glycosylation	618:636	Asn-N-glycosylation of MICA	618:644	MATERIALS AND METHODS The cell-surface expression and Asn-N-glycosylation of MICA were estimated by flow cytometry, and western blot analyses, respectively.
29491059	4	33	theme	MICA	641:644	arg1	expression					603:612	The cell-surface expression	586:612	The cell-surface expression	586:612	MATERIALS AND METHODS The cell-surface expression and Asn-N-glycosylation of MICA were estimated by flow cytometry, and western blot analyses, respectively.
29491059	4	33	theme	MICA	641:644	arg1	MATERIALS					564:572	MATERIALS	564:572	MATERIALS	564:572	MATERIALS AND METHODS The cell-surface expression and Asn-N-glycosylation of MICA were estimated by flow cytometry, and western blot analyses, respectively.
29491059	7	34	theme	expression	1201:1210	arg1	vectors					1212:1218	mutant MICA expression vectors	1189:1218	mutant MICA expression vectors designed for abrogation of N-linked glycosylation	1189:1268	Cells transfected with wild-type MICA expression vector expressed cell surface MICA more than those transfected with mutant MICA expression vectors designed for abrogation of N-linked glycosylation.
29491059	1	35	dep	surface	188:194	arg1	MICA					255:258	MICA	255:258	MICA	255:258	BACKGROUND/AIM Hypoxia down-regulates the expression of cell surface major histocompatibility class I-related chain molecule A (MICA) without increasing its shedding.
29491059	1	35	dep	surface	188:194	arg1	A					252:252	major histocompatibility class I-related chain molecule A	196:252	cell surface major histocompatibility class I-related chain molecule A (MICA)	183:259	BACKGROUND/AIM Hypoxia down-regulates the expression of cell surface major histocompatibility class I-related chain molecule A (MICA) without increasing its shedding.
29491059	5	36	link	N-linked	763:770	arg1	MICA					785:788	N-linked glycosylated MICA	763:788	N-linked glycosylated MICA	763:788	RESULTS Hypoxia reduced the expression of N-linked glycosylated MICA, as well as the ratio of N-linked glycosylated to non-glycosylated MICA.
29491059	3	37	theme	MICA	533:536	arg1	down-regulation					501:515	hypoxia-induced down-regulation	485:515	hypoxia-induced down-regulation of cell-surface MICA	485:536	We investigated the participation of asparagine (Asn)-linked glycosylation in hypoxia-induced down-regulation of cell-surface MICA using osteosarcoma cells.
29491059	0	38	theme	Asparagine-linked	14:30	arg1	Glycosylation					32:44	Asparagine-linked Glycosylation	14:44	Asparagine-linked Glycosylation	14:44	Inhibition of Asparagine-linked Glycosylation Participates in Hypoxia-induced Down-regulation of Cell-surface MICA Expression.
29491059	7	39	theme	MICA	1105:1108	arg1	vector					1121:1126	wild-type MICA expression vector	1095:1126	wild-type MICA expression vector	1095:1126	Cells transfected with wild-type MICA expression vector expressed cell surface MICA more than those transfected with mutant MICA expression vectors designed for abrogation of N-linked glycosylation.
29491059	1	40	theme	class	221:225	arg1	MICA					255:258	MICA	255:258	MICA	255:258	BACKGROUND/AIM Hypoxia down-regulates the expression of cell surface major histocompatibility class I-related chain molecule A (MICA) without increasing its shedding.
29491059	1	40	theme	class	221:225	arg1	A					252:252	major histocompatibility class I-related chain molecule A	196:252	cell surface major histocompatibility class I-related chain molecule A (MICA)	183:259	BACKGROUND/AIM Hypoxia down-regulates the expression of cell surface major histocompatibility class I-related chain molecule A (MICA) without increasing its shedding.
29491059	5	41	gly	glycosylated	772:783	arg1	MICA					785:788	N-linked glycosylated MICA	763:788	N-linked glycosylated MICA	763:788	RESULTS Hypoxia reduced the expression of N-linked glycosylated MICA, as well as the ratio of N-linked glycosylated to non-glycosylated MICA.
29491059	8	42	theme	MICA	1405:1408	arg1	expression					1391:1400	cell-surface expression	1378:1400	cell-surface expression of MICA	1378:1408	CONCLUSION The inhibition of Asn-N-linked glycosylation participates in hypoxia-induced down-regulation of cell-surface expression of MICA.
29491059	7	43	theme	mutant	1189:1194	arg1	vectors					1212:1218	mutant MICA expression vectors	1189:1218	mutant MICA expression vectors designed for abrogation of N-linked glycosylation	1189:1268	Cells transfected with wild-type MICA expression vector expressed cell surface MICA more than those transfected with mutant MICA expression vectors designed for abrogation of N-linked glycosylation.
29491059	3	44	theme	hypoxia-induced	485:499	arg1	down-regulation					501:515	hypoxia-induced down-regulation	485:515	hypoxia-induced down-regulation of cell-surface MICA	485:536	We investigated the participation of asparagine (Asn)-linked glycosylation in hypoxia-induced down-regulation of cell-surface MICA using osteosarcoma cells.
29491059	3	45	theme	-linked	460:466	arg1	glycosylation					468:480	asparagine (Asn)-linked glycosylation	444:480	asparagine (Asn)-linked glycosylation	444:480	We investigated the participation of asparagine (Asn)-linked glycosylation in hypoxia-induced down-regulation of cell-surface MICA using osteosarcoma cells.
29491059	8	46	theme	expression	1391:1400	arg1	down-regulation					1359:1373	hypoxia-induced down-regulation	1343:1373	hypoxia-induced down-regulation of cell-surface expression of MICA	1343:1408	CONCLUSION The inhibition of Asn-N-linked glycosylation participates in hypoxia-induced down-regulation of cell-surface expression of MICA.
29491059	8	47	link	Asn-N-linked	1300:1311	arg1	glycosylation					1313:1325	Asn-N-linked glycosylation	1300:1325	Asn-N-linked glycosylation	1300:1325	CONCLUSION The inhibition of Asn-N-linked glycosylation participates in hypoxia-induced down-regulation of cell-surface expression of MICA.
29491059	3	48	theme	cell-surface	520:531	arg1	MICA					533:536	cell-surface MICA	520:536	cell-surface MICA	520:536	We investigated the participation of asparagine (Asn)-linked glycosylation in hypoxia-induced down-regulation of cell-surface MICA using osteosarcoma cells.
29491059	5	49	theme	N-linked	763:770	arg1	MICA					785:788	N-linked glycosylated MICA	763:788	N-linked glycosylated MICA	763:788	RESULTS Hypoxia reduced the expression of N-linked glycosylated MICA, as well as the ratio of N-linked glycosylated to non-glycosylated MICA.
29491059	6	50	link	N-linked	897:904	arg1	glycosylation					906:918	N-linked glycosylation	897:918	N-linked glycosylation	897:918	2-Deoxy-D-glucose, which inhibits N-linked glycosylation, reduced the cell-surface expression of MICA under normoxia, while D-Mannose increased N-glycosylated MICA, increasing cell-surface MICA under hypoxia.
29491059	7	51	theme	MICA	1196:1199	arg1	vectors					1212:1218	mutant MICA expression vectors	1189:1218	mutant MICA expression vectors designed for abrogation of N-linked glycosylation	1189:1268	Cells transfected with wild-type MICA expression vector expressed cell surface MICA more than those transfected with mutant MICA expression vectors designed for abrogation of N-linked glycosylation.
29491059	0	52	theme	Hypoxia-induced	62:76	arg1	Down-regulation					78:92	Hypoxia-induced Down-regulation	62:92	Hypoxia-induced Down-regulation of Cell-surface MICA Expression	62:124	Inhibition of Asparagine-linked Glycosylation Participates in Hypoxia-induced Down-regulation of Cell-surface MICA Expression.
29491059	5	53	theme	glycosylated	772:783	arg1	MICA					785:788	N-linked glycosylated MICA	763:788	N-linked glycosylated MICA	763:788	RESULTS Hypoxia reduced the expression of N-linked glycosylated MICA, as well as the ratio of N-linked glycosylated to non-glycosylated MICA.
29491059	3	54	link	-linked	460:466	arg1	glycosylation					468:480	asparagine (Asn)-linked glycosylation	444:480	asparagine (Asn)-linked glycosylation	444:480	We investigated the participation of asparagine (Asn)-linked glycosylation in hypoxia-induced down-regulation of cell-surface MICA using osteosarcoma cells.
29491059	7	55	theme	cell	1138:1141	arg1	MICA					1151:1154	cell surface MICA	1138:1154	cell surface MICA	1138:1154	Cells transfected with wild-type MICA expression vector expressed cell surface MICA more than those transfected with mutant MICA expression vectors designed for abrogation of N-linked glycosylation.
29491059	5	56	theme	MICA	785:788	arg1	ratio					806:810	the ratio	802:810	the ratio of N-linked	802:822	RESULTS Hypoxia reduced the expression of N-linked glycosylated MICA, as well as the ratio of N-linked glycosylated to non-glycosylated MICA.
29491059	5	56	theme	MICA	785:788	arg1	expression					749:758	the expression	745:758	the expression of N-linked glycosylated MICA	745:788	RESULTS Hypoxia reduced the expression of N-linked glycosylated MICA, as well as the ratio of N-linked glycosylated to non-glycosylated MICA.
29491059	7	57	theme	surface	1143:1149	arg1	MICA					1151:1154	cell surface MICA	1138:1154	cell surface MICA	1138:1154	Cells transfected with wild-type MICA expression vector expressed cell surface MICA more than those transfected with mutant MICA expression vectors designed for abrogation of N-linked glycosylation.
29491059	2	58	theme	cell-surface	374:385	arg1	expression					387:396	the cell-surface expression	370:396	the cell-surface expression of MICA	370:404	Recently, the inhibition of N-linked glycosylation was also shown to reduce the cell-surface expression of MICA.
29491059	6	59	gly	N-glycosylated	1007:1020	arg1	MICA					1022:1025	N-glycosylated MICA	1007:1025	N-glycosylated MICA	1007:1025	2-Deoxy-D-glucose, which inhibits N-linked glycosylation, reduced the cell-surface expression of MICA under normoxia, while D-Mannose increased N-glycosylated MICA, increasing cell-surface MICA under hypoxia.
29491059	4	60	theme	blot	692:695	arg1	analyses					697:704	western blot analyses	684:704	western blot analyses	684:704	MATERIALS AND METHODS The cell-surface expression and Asn-N-glycosylation of MICA were estimated by flow cytometry, and western blot analyses, respectively.
29491059	6	61	theme	cell-surface	933:944	arg1	expression					946:955	the cell-surface expression	929:955	the cell-surface expression of MICA	929:963	2-Deoxy-D-glucose, which inhibits N-linked glycosylation, reduced the cell-surface expression of MICA under normoxia, while D-Mannose increased N-glycosylated MICA, increasing cell-surface MICA under hypoxia.
29491059	1	62	theme	cell	183:186	arg1	surface					188:194	cell surface major histocompatibility class I-related chain molecule A (MICA)	183:259	cell surface major histocompatibility class I-related chain molecule A (MICA)	183:259	BACKGROUND/AIM Hypoxia down-regulates the expression of cell surface major histocompatibility class I-related chain molecule A (MICA) without increasing its shedding.
29491059	3	63	theme	osteosarcoma	544:555	arg1	cells					557:561	osteosarcoma cells	544:561	osteosarcoma cells	544:561	We investigated the participation of asparagine (Asn)-linked glycosylation in hypoxia-induced down-regulation of cell-surface MICA using osteosarcoma cells.
29491059	0	64	theme	Cell-surface	97:108	arg1	Expression					115:124	Cell-surface MICA Expression	97:124	Cell-surface MICA Expression	97:124	Inhibition of Asparagine-linked Glycosylation Participates in Hypoxia-induced Down-regulation of Cell-surface MICA Expression.
29491059	7	65	link	N-linked	1247:1254	arg1	glycosylation					1256:1268	N-linked glycosylation	1247:1268	N-linked glycosylation	1247:1268	Cells transfected with wild-type MICA expression vector expressed cell surface MICA more than those transfected with mutant MICA expression vectors designed for abrogation of N-linked glycosylation.
29491059	1	66	theme	surface	188:194	arg1	expression					169:178	the expression	165:178	the expression of cell surface major histocompatibility class I-related chain molecule A (MICA)	165:259	BACKGROUND/AIM Hypoxia down-regulates the expression of cell surface major histocompatibility class I-related chain molecule A (MICA) without increasing its shedding.
29491059	5	67	theme	non-glycosylated	840:855	arg1	MICA					857:860	non-glycosylated MICA	840:860	non-glycosylated MICA	840:860	RESULTS Hypoxia reduced the expression of N-linked glycosylated MICA, as well as the ratio of N-linked glycosylated to non-glycosylated MICA.
29491059	6	68	theme	cell-surface	1039:1050	arg1	MICA					1052:1055	cell-surface MICA	1039:1055	cell-surface MICA	1039:1055	2-Deoxy-D-glucose, which inhibits N-linked glycosylation, reduced the cell-surface expression of MICA under normoxia, while D-Mannose increased N-glycosylated MICA, increasing cell-surface MICA under hypoxia.
29491059	8	69	theme	cell-surface	1378:1389	arg1	expression					1391:1400	cell-surface expression	1378:1400	cell-surface expression of MICA	1378:1408	CONCLUSION The inhibition of Asn-N-linked glycosylation participates in hypoxia-induced down-regulation of cell-surface expression of MICA.
29491059	4	70	gly	Asn-N-glycosylation	618:636	arg1	MICA					641:644	MICA	641:644	MICA	641:644	MATERIALS AND METHODS The cell-surface expression and Asn-N-glycosylation of MICA were estimated by flow cytometry, and western blot analyses, respectively.
29491059	1	71	theme	major	196:200	arg1	class					221:225	major histocompatibility class	196:225	cell surface major histocompatibility class I-related chain molecule A (MICA)	183:259	BACKGROUND/AIM Hypoxia down-regulates the expression of cell surface major histocompatibility class I-related chain molecule A (MICA) without increasing its shedding.
31533338	4	0	theme	cell	663:666	arg1	lines					668:672	oral squamous cell carcinoma cell lines	634:672	oral squamous cell carcinoma cell lines	634:672	To further understand the overall effect of and metabolic alteration by 2DG, we performed biochemical and metabolomics analyses on oral squamous cell carcinoma cell lines.
31533338	2	1	theme	potent	256:261	arg1	2-deoxy-d-glucose					288:304	2-deoxy-d-glucose	288:304	2-deoxy-d-glucose (2DG)	288:310	As a potent inhibitor of glycolysis, 2-deoxy-d-glucose (2DG) has been proposed for cancer treatment and extensively investigated in clinical studies.
31533338	2	1	theme	potent	256:261	arg1	inhibitor					263:271	a potent inhibitor	254:271	a potent inhibitor of glycolysis	254:285	As a potent inhibitor of glycolysis, 2-deoxy-d-glucose (2DG) has been proposed for cancer treatment and extensively investigated in clinical studies.
31533338	9	2	theme	metabolites	1465:1475	arg1	alterations					1444:1454	alterations	1444:1454	alterations of these metabolites	1444:1475	These observations suggest that alterations of these metabolites may be responsible for the phenotypic and metabolic changes in SCC15 cells induced by 2DG.
31533338	7	3	theme	N-linked	1064:1071	arg1	glycosylation					1073:1085	N-linked glycosylation	1064:1085	N-linked glycosylation	1064:1085	The impaired processes of glycolysis and N-linked glycosylation were restored by exogenous addition of pyruvate and mannose, respectively.
31533338	8	4	theme	metabolomics	1189:1200	arg1	analysis					1202:1209	our targeted metabolomics analysis	1176:1209	our targeted metabolomics analysis	1176:1209	Additionally, our targeted metabolomics analysis revealed significant alterations in the metabolites, including amino acids, biogenic amines, glycerophospholipids, and sphingolipids, caused by the impairment of glycolysis and N-linked glycosylation.
31533338	6	5	theme	SCC15	944:948	arg1	cells					950:954	SCC15 cells	944:954	SCC15 cells	944:954	Coincidentally, 2DG impaired N-linked glycosylation of the key oncogenic receptors Axl and Met in SCC15 cells, thereby reducing the cell viability and colony formation ability.
31533338	4	6	theme	oral	634:637	arg1	lines					668:672	oral squamous cell carcinoma cell lines	634:672	oral squamous cell carcinoma cell lines	634:672	To further understand the overall effect of and metabolic alteration by 2DG, we performed biochemical and metabolomics analyses on oral squamous cell carcinoma cell lines.
31533338	10	7	theme	N-linked	1600:1607	arg1	glycosylation					1609:1621	N-linked glycosylation	1600:1621	N-linked glycosylation of Axl and Met	1600:1636	Moreover, our data suggest that N-linked glycosylation of Axl and Met may contribute to the maintenance of cancer properties in SCC15 cells.
31533338	11	8	theme	novel	1799:1803	arg1	targets					1817:1823	novel therapeutic targets	1799:1823	novel therapeutic targets for treating human oral cancer	1799:1854	Further studies are needed to elucidate the roles of these altered metabolites to provide novel therapeutic targets for treating human oral cancer.
31533338	7	9	theme	glycolysis	1049:1058	arg1	processes					1036:1044	The impaired processes	1023:1044	The impaired processes of glycolysis and N-linked glycosylation	1023:1085	The impaired processes of glycolysis and N-linked glycosylation were restored by exogenous addition of pyruvate and mannose, respectively.
31533338	4	10	theme	cell	648:651	arg1	lines					668:672	oral squamous cell carcinoma cell lines	634:672	oral squamous cell carcinoma cell lines	634:672	To further understand the overall effect of and metabolic alteration by 2DG, we performed biochemical and metabolomics analyses on oral squamous cell carcinoma cell lines.
31533338	11	11	theme	oral	1844:1847	arg1	cancer					1849:1854	human oral cancer	1838:1854	human oral cancer	1838:1854	Further studies are needed to elucidate the roles of these altered metabolites to provide novel therapeutic targets for treating human oral cancer.
31533338	9	12	theme	phenotypic	1504:1513	arg1	changes					1529:1535	the phenotypic and metabolic changes	1500:1535	the phenotypic and metabolic changes in SCC15 cells induced by 2DG	1500:1565	These observations suggest that alterations of these metabolites may be responsible for the phenotypic and metabolic changes in SCC15 cells induced by 2DG.
31533338	9	13	theme	metabolic	1519:1527	arg1	changes					1529:1535	the phenotypic and metabolic changes	1500:1535	the phenotypic and metabolic changes in SCC15 cells induced by 2DG	1500:1565	These observations suggest that alterations of these metabolites may be responsible for the phenotypic and metabolic changes in SCC15 cells induced by 2DG.
31533338	6	14	theme	oncogenic	909:917	arg1	Axl					929:931	Axl	929:931	Axl	929:931	Coincidentally, 2DG impaired N-linked glycosylation of the key oncogenic receptors Axl and Met in SCC15 cells, thereby reducing the cell viability and colony formation ability.
31533338	6	14	theme	oncogenic	909:917	arg1	receptors					919:927	the key oncogenic receptors	901:927	the key oncogenic receptors Axl and Met	901:939	Coincidentally, 2DG impaired N-linked glycosylation of the key oncogenic receptors Axl and Met in SCC15 cells, thereby reducing the cell viability and colony formation ability.
31533338	6	14	theme	oncogenic	909:917	arg1	Met					937:939	Met	937:939	Met	937:939	Coincidentally, 2DG impaired N-linked glycosylation of the key oncogenic receptors Axl and Met in SCC15 cells, thereby reducing the cell viability and colony formation ability.
31533338	5	15	theme	SCC15	774:778	arg1	cells					780:784	SCC15 cells	774:784	SCC15 cells	774:784	In this study, we found that 2DG more effectively reduced glucose consumption and lactate level in SCC15 cells than in SCC4 cells, which are less dependent on glycolysis.
31533338	6	16	theme	cell	978:981	arg1	viability					983:991	the cell viability	974:991	the cell viability	974:991	Coincidentally, 2DG impaired N-linked glycosylation of the key oncogenic receptors Axl and Met in SCC15 cells, thereby reducing the cell viability and colony formation ability.
31533338	0	17	from	Alteration	36:45	arg1	Cells					76:80	Human Oral Squamous SCC15 Cells	50:80	Human Oral Squamous SCC15 Cells	50:80	2-Deoxy-d-Glucose-Induced Metabolic Alteration in Human Oral Squamous SCC15 Cells: Involvement of N-Glycosylation of Axl and Met.
31533338	8	18	from	alterations	1232:1242	arg1	metabolites					1251:1261	the metabolites	1247:1261	the metabolites	1247:1261	Additionally, our targeted metabolomics analysis revealed significant alterations in the metabolites, including amino acids, biogenic amines, glycerophospholipids, and sphingolipids, caused by the impairment of glycolysis and N-linked glycosylation.
31533338	8	18	from	alterations	1232:1242	arg1	sphingolipids					1330:1342	sphingolipids	1330:1342	sphingolipids	1330:1342	Additionally, our targeted metabolomics analysis revealed significant alterations in the metabolites, including amino acids, biogenic amines, glycerophospholipids, and sphingolipids, caused by the impairment of glycolysis and N-linked glycosylation.
31533338	8	18	from	alterations	1232:1242	arg1	glycerophospholipids					1304:1323	glycerophospholipids	1304:1323	glycerophospholipids	1304:1323	Additionally, our targeted metabolomics analysis revealed significant alterations in the metabolites, including amino acids, biogenic amines, glycerophospholipids, and sphingolipids, caused by the impairment of glycolysis and N-linked glycosylation.
31533338	8	18	from	alterations	1232:1242	arg1	acids					1280:1284	amino acids	1274:1284	amino acids	1274:1284	Additionally, our targeted metabolomics analysis revealed significant alterations in the metabolites, including amino acids, biogenic amines, glycerophospholipids, and sphingolipids, caused by the impairment of glycolysis and N-linked glycosylation.
31533338	8	18	from	alterations	1232:1242	arg1	amines					1296:1301	biogenic amines	1287:1301	biogenic amines	1287:1301	Additionally, our targeted metabolomics analysis revealed significant alterations in the metabolites, including amino acids, biogenic amines, glycerophospholipids, and sphingolipids, caused by the impairment of glycolysis and N-linked glycosylation.
31533338	1	19	theme	hallmarks	156:164	arg1	One					130:132	One	130:132	One	130:132	One of the most prominent hallmarks of cancer cells is their dependency on the glycolytic pathway for energy production.
31533338	1	19	theme	hallmarks	156:164	arg1	hallmarks					156:164	the most prominent hallmarks	137:164	the most prominent hallmarks of cancer cells	137:180	One of the most prominent hallmarks of cancer cells is their dependency on the glycolytic pathway for energy production.
31533338	11	20	theme	altered	1768:1774	arg1	metabolites					1776:1786	these altered metabolites	1762:1786	these altered metabolites	1762:1786	Further studies are needed to elucidate the roles of these altered metabolites to provide novel therapeutic targets for treating human oral cancer.
31533338	0	21	theme	N-Glycosylation	98:112	arg1	Involvement					83:93	Involvement	83:93	2-Deoxy-d-Glucose-Induced Metabolic Alteration in Human Oral Squamous SCC15 Cells: Involvement of N-Glycosylation of Axl and Met.	0:128	2-Deoxy-d-Glucose-Induced Metabolic Alteration in Human Oral Squamous SCC15 Cells: Involvement of N-Glycosylation of Axl and Met.
31533338	2	22	theme	clinical	383:390	arg1	studies					392:398	clinical studies	383:398	clinical studies	383:398	As a potent inhibitor of glycolysis, 2-deoxy-d-glucose (2DG) has been proposed for cancer treatment and extensively investigated in clinical studies.
31533338	5	23	theme	glucose	733:739	arg1	consumption					741:751	glucose consumption	733:751	glucose consumption	733:751	In this study, we found that 2DG more effectively reduced glucose consumption and lactate level in SCC15 cells than in SCC4 cells, which are less dependent on glycolysis.
31533338	8	24	theme	glycolysis	1373:1382	arg1	impairment					1359:1368	the impairment	1355:1368	the impairment of glycolysis and N-linked glycosylation	1355:1409	Additionally, our targeted metabolomics analysis revealed significant alterations in the metabolites, including amino acids, biogenic amines, glycerophospholipids, and sphingolipids, caused by the impairment of glycolysis and N-linked glycosylation.
31533338	0	25	theme	2-Deoxy-d-Glucose-Induced	0:24	arg1	Alteration					36:45	2-Deoxy-d-Glucose-Induced Metabolic Alteration	0:45	2-Deoxy-d-Glucose-Induced Metabolic Alteration in Human Oral Squamous SCC15 Cells: Involvement of N-Glycosylation of Axl and Met.	0:128	2-Deoxy-d-Glucose-Induced Metabolic Alteration in Human Oral Squamous SCC15 Cells: Involvement of N-Glycosylation of Axl and Met.
31533338	10	26	gly	glycosylation	1609:1621	arg1	properties					1682:1691	cancer properties	1675:1691	cancer properties	1675:1691	Moreover, our data suggest that N-linked glycosylation of Axl and Met may contribute to the maintenance of cancer properties in SCC15 cells.
31533338	10	26	gly	glycosylation	1609:1621	arg1	Axl					1626:1628	Axl	1626:1628	Axl	1626:1628	Moreover, our data suggest that N-linked glycosylation of Axl and Met may contribute to the maintenance of cancer properties in SCC15 cells.
31533338	10	26	gly	glycosylation	1609:1621	arg1	Met					1634:1636	Met	1634:1636	Met	1634:1636	Moreover, our data suggest that N-linked glycosylation of Axl and Met may contribute to the maintenance of cancer properties in SCC15 cells.
31533338	8	27	theme	N-linked	1388:1395	arg1	glycosylation					1397:1409	N-linked glycosylation	1388:1409	N-linked glycosylation	1388:1409	Additionally, our targeted metabolomics analysis revealed significant alterations in the metabolites, including amino acids, biogenic amines, glycerophospholipids, and sphingolipids, caused by the impairment of glycolysis and N-linked glycosylation.
31533338	10	28	theme	SCC15	1696:1700	arg1	cells					1702:1706	SCC15 cells	1696:1706	SCC15 cells	1696:1706	Moreover, our data suggest that N-linked glycosylation of Axl and Met may contribute to the maintenance of cancer properties in SCC15 cells.
31533338	6	29	link	N-linked	875:882	arg1	glycosylation					884:896	N-linked glycosylation	875:896	N-linked glycosylation of the key oncogenic receptors Axl and Met in SCC15 cells	875:954	Coincidentally, 2DG impaired N-linked glycosylation of the key oncogenic receptors Axl and Met in SCC15 cells, thereby reducing the cell viability and colony formation ability.
31533338	10	30	theme	properties	1682:1691	arg1	maintenance					1660:1670	the maintenance	1656:1670	the maintenance of cancer properties in SCC15 cells	1656:1706	Moreover, our data suggest that N-linked glycosylation of Axl and Met may contribute to the maintenance of cancer properties in SCC15 cells.
31533338	7	31	theme	mannose	1139:1145	arg1	addition					1114:1121	exogenous addition	1104:1121	exogenous addition of pyruvate and mannose	1104:1145	The impaired processes of glycolysis and N-linked glycosylation were restored by exogenous addition of pyruvate and mannose, respectively.
31533338	0	32	theme	Human	50:54	arg1	Cells					76:80	Human Oral Squamous SCC15 Cells	50:80	Human Oral Squamous SCC15 Cells	50:80	2-Deoxy-d-Glucose-Induced Metabolic Alteration in Human Oral Squamous SCC15 Cells: Involvement of N-Glycosylation of Axl and Met.
31533338	6	33	theme	formation	1004:1012	arg1	ability					1014:1020	colony formation ability	997:1020	colony formation ability	997:1020	Coincidentally, 2DG impaired N-linked glycosylation of the key oncogenic receptors Axl and Met in SCC15 cells, thereby reducing the cell viability and colony formation ability.
31533338	0	34	theme	Squamous	61:68	arg1	Cells					76:80	Human Oral Squamous SCC15 Cells	50:80	Human Oral Squamous SCC15 Cells	50:80	2-Deoxy-d-Glucose-Induced Metabolic Alteration in Human Oral Squamous SCC15 Cells: Involvement of N-Glycosylation of Axl and Met.
31533338	3	35	theme	biological	457:466	arg1	glycosylation					488:500	glycosylation	488:500	glycosylation	488:500	Moreover, 2DG has been reported to interfere with other biological processes including glycosylation.
31533338	3	35	theme	biological	457:466	arg1	processes					468:476	other biological processes	451:476	other biological processes including glycosylation	451:500	Moreover, 2DG has been reported to interfere with other biological processes including glycosylation.
31533338	1	36	theme	glycolytic	209:218	arg1	pathway					220:226	the glycolytic pathway	205:226	the glycolytic pathway for energy production	205:248	One of the most prominent hallmarks of cancer cells is their dependency on the glycolytic pathway for energy production.
31533338	0	37	dep	Alteration	36:45	arg1	Involvement					83:93	Involvement	83:93	2-Deoxy-d-Glucose-Induced Metabolic Alteration in Human Oral Squamous SCC15 Cells: Involvement of N-Glycosylation of Axl and Met.	0:128	2-Deoxy-d-Glucose-Induced Metabolic Alteration in Human Oral Squamous SCC15 Cells: Involvement of N-Glycosylation of Axl and Met.
31533338	10	38	theme	cancer	1675:1680	arg1	properties					1682:1691	cancer properties	1675:1691	cancer properties	1675:1691	Moreover, our data suggest that N-linked glycosylation of Axl and Met may contribute to the maintenance of cancer properties in SCC15 cells.
31533338	1	39	theme	cancer	169:174	arg1	cells					176:180	cancer cells	169:180	cancer cells	169:180	One of the most prominent hallmarks of cancer cells is their dependency on the glycolytic pathway for energy production.
31533338	6	40	theme	key	905:907	arg1	Axl					929:931	Axl	929:931	Axl	929:931	Coincidentally, 2DG impaired N-linked glycosylation of the key oncogenic receptors Axl and Met in SCC15 cells, thereby reducing the cell viability and colony formation ability.
31533338	6	40	theme	key	905:907	arg1	receptors					919:927	the key oncogenic receptors	901:927	the key oncogenic receptors Axl and Met	901:939	Coincidentally, 2DG impaired N-linked glycosylation of the key oncogenic receptors Axl and Met in SCC15 cells, thereby reducing the cell viability and colony formation ability.
31533338	6	40	theme	key	905:907	arg1	Met					937:939	Met	937:939	Met	937:939	Coincidentally, 2DG impaired N-linked glycosylation of the key oncogenic receptors Axl and Met in SCC15 cells, thereby reducing the cell viability and colony formation ability.
31533338	7	41	theme	exogenous	1104:1112	arg1	addition					1114:1121	exogenous addition	1104:1121	exogenous addition of pyruvate and mannose	1104:1145	The impaired processes of glycolysis and N-linked glycosylation were restored by exogenous addition of pyruvate and mannose, respectively.
31533338	2	42	theme	cancer	334:339	arg1	treatment					341:349	cancer treatment	334:349	cancer treatment	334:349	As a potent inhibitor of glycolysis, 2-deoxy-d-glucose (2DG) has been proposed for cancer treatment and extensively investigated in clinical studies.
31533338	10	43	link	N-linked	1600:1607	arg1	glycosylation					1609:1621	N-linked glycosylation	1600:1621	N-linked glycosylation of Axl and Met	1600:1636	Moreover, our data suggest that N-linked glycosylation of Axl and Met may contribute to the maintenance of cancer properties in SCC15 cells.
31533338	2	44	theme	glycolysis	276:285	arg1	2-deoxy-d-glucose					288:304	2-deoxy-d-glucose	288:304	2-deoxy-d-glucose (2DG)	288:310	As a potent inhibitor of glycolysis, 2-deoxy-d-glucose (2DG) has been proposed for cancer treatment and extensively investigated in clinical studies.
31533338	2	44	theme	glycolysis	276:285	arg1	inhibitor					263:271	a potent inhibitor	254:271	a potent inhibitor of glycolysis	254:285	As a potent inhibitor of glycolysis, 2-deoxy-d-glucose (2DG) has been proposed for cancer treatment and extensively investigated in clinical studies.
31533338	6	45	theme	N-linked	875:882	arg1	glycosylation					884:896	N-linked glycosylation	875:896	N-linked glycosylation of the key oncogenic receptors Axl and Met in SCC15 cells	875:954	Coincidentally, 2DG impaired N-linked glycosylation of the key oncogenic receptors Axl and Met in SCC15 cells, thereby reducing the cell viability and colony formation ability.
31533338	7	46	theme	glycosylation	1073:1085	arg1	processes					1036:1044	The impaired processes	1023:1044	The impaired processes of glycolysis and N-linked glycosylation	1023:1085	The impaired processes of glycolysis and N-linked glycosylation were restored by exogenous addition of pyruvate and mannose, respectively.
31533338	7	47	theme	pyruvate	1126:1133	arg1	addition					1114:1121	exogenous addition	1104:1121	exogenous addition of pyruvate and mannose	1104:1145	The impaired processes of glycolysis and N-linked glycosylation were restored by exogenous addition of pyruvate and mannose, respectively.
31533338	9	48	from	changes	1529:1535	arg1	cells					1546:1550	SCC15 cells	1540:1550	SCC15 cells induced by 2DG	1540:1565	These observations suggest that alterations of these metabolites may be responsible for the phenotypic and metabolic changes in SCC15 cells induced by 2DG.
31533338	4	49	theme	carcinoma	653:661	arg1	lines					668:672	oral squamous cell carcinoma cell lines	634:672	oral squamous cell carcinoma cell lines	634:672	To further understand the overall effect of and metabolic alteration by 2DG, we performed biochemical and metabolomics analyses on oral squamous cell carcinoma cell lines.
31533338	10	50	theme	Axl	1626:1628	arg1	glycosylation					1609:1621	N-linked glycosylation	1600:1621	N-linked glycosylation of Axl and Met	1600:1636	Moreover, our data suggest that N-linked glycosylation of Axl and Met may contribute to the maintenance of cancer properties in SCC15 cells.
31533338	1	51	theme	cells	176:180	arg1	hallmarks					156:164	the most prominent hallmarks	137:164	the most prominent hallmarks of cancer cells	137:180	One of the most prominent hallmarks of cancer cells is their dependency on the glycolytic pathway for energy production.
31533338	8	52	theme	targeted	1180:1187	arg1	analysis					1202:1209	our targeted metabolomics analysis	1176:1209	our targeted metabolomics analysis	1176:1209	Additionally, our targeted metabolomics analysis revealed significant alterations in the metabolites, including amino acids, biogenic amines, glycerophospholipids, and sphingolipids, caused by the impairment of glycolysis and N-linked glycosylation.
31533338	6	53	dep	receptors	919:927	arg1	Axl					929:931	Axl	929:931	Axl	929:931	Coincidentally, 2DG impaired N-linked glycosylation of the key oncogenic receptors Axl and Met in SCC15 cells, thereby reducing the cell viability and colony formation ability.
31533338	6	53	dep	receptors	919:927	arg1	receptors					919:927	the key oncogenic receptors	901:927	the key oncogenic receptors Axl and Met	901:939	Coincidentally, 2DG impaired N-linked glycosylation of the key oncogenic receptors Axl and Met in SCC15 cells, thereby reducing the cell viability and colony formation ability.
31533338	6	53	dep	receptors	919:927	arg1	Met					937:939	Met	937:939	Met	937:939	Coincidentally, 2DG impaired N-linked glycosylation of the key oncogenic receptors Axl and Met in SCC15 cells, thereby reducing the cell viability and colony formation ability.
31533338	11	54	theme	therapeutic	1805:1815	arg1	targets					1817:1823	novel therapeutic targets	1799:1823	novel therapeutic targets for treating human oral cancer	1799:1854	Further studies are needed to elucidate the roles of these altered metabolites to provide novel therapeutic targets for treating human oral cancer.
31533338	7	55	theme	impaired	1027:1034	arg1	processes					1036:1044	The impaired processes	1023:1044	The impaired processes of glycolysis and N-linked glycosylation	1023:1085	The impaired processes of glycolysis and N-linked glycosylation were restored by exogenous addition of pyruvate and mannose, respectively.
31533338	8	56	link	N-linked	1388:1395	arg1	glycosylation					1397:1409	N-linked glycosylation	1388:1409	N-linked glycosylation	1388:1409	Additionally, our targeted metabolomics analysis revealed significant alterations in the metabolites, including amino acids, biogenic amines, glycerophospholipids, and sphingolipids, caused by the impairment of glycolysis and N-linked glycosylation.
31533338	4	57	theme	squamous	639:646	arg1	lines					668:672	oral squamous cell carcinoma cell lines	634:672	oral squamous cell carcinoma cell lines	634:672	To further understand the overall effect of and metabolic alteration by 2DG, we performed biochemical and metabolomics analyses on oral squamous cell carcinoma cell lines.
31533338	10	58	theme	Met	1634:1636	arg1	glycosylation					1609:1621	N-linked glycosylation	1600:1621	N-linked glycosylation of Axl and Met	1600:1636	Moreover, our data suggest that N-linked glycosylation of Axl and Met may contribute to the maintenance of cancer properties in SCC15 cells.
31533338	8	59	theme	significant	1220:1230	arg1	alterations					1232:1242	significant alterations	1220:1242	significant alterations	1220:1242	Additionally, our targeted metabolomics analysis revealed significant alterations in the metabolites, including amino acids, biogenic amines, glycerophospholipids, and sphingolipids, caused by the impairment of glycolysis and N-linked glycosylation.
31533338	6	60	theme	receptors	919:927	arg1	glycosylation					884:896	N-linked glycosylation	875:896	N-linked glycosylation of the key oncogenic receptors Axl and Met in SCC15 cells	875:954	Coincidentally, 2DG impaired N-linked glycosylation of the key oncogenic receptors Axl and Met in SCC15 cells, thereby reducing the cell viability and colony formation ability.
31533338	8	61	theme	biogenic	1287:1294	arg1	amines					1296:1301	biogenic amines	1287:1301	biogenic amines	1287:1301	Additionally, our targeted metabolomics analysis revealed significant alterations in the metabolites, including amino acids, biogenic amines, glycerophospholipids, and sphingolipids, caused by the impairment of glycolysis and N-linked glycosylation.
31533338	11	62	theme	human	1838:1842	arg1	cancer					1849:1854	human oral cancer	1838:1854	human oral cancer	1838:1854	Further studies are needed to elucidate the roles of these altered metabolites to provide novel therapeutic targets for treating human oral cancer.
31533338	1	63	from	dependency	191:200	arg1	pathway					220:226	the glycolytic pathway	205:226	the glycolytic pathway for energy production	205:248	One of the most prominent hallmarks of cancer cells is their dependency on the glycolytic pathway for energy production.
31533338	6	64	theme	colony	997:1002	arg1	ability					1014:1020	colony formation ability	997:1020	colony formation ability	997:1020	Coincidentally, 2DG impaired N-linked glycosylation of the key oncogenic receptors Axl and Met in SCC15 cells, thereby reducing the cell viability and colony formation ability.
31533338	4	65	dep	biochemical	593:603	arg1	analyses					622:629	analyses	622:629	analyses	622:629	To further understand the overall effect of and metabolic alteration by 2DG, we performed biochemical and metabolomics analyses on oral squamous cell carcinoma cell lines.
31533338	8	66	theme	amino	1274:1278	arg1	acids					1280:1284	amino acids	1274:1284	amino acids	1274:1284	Additionally, our targeted metabolomics analysis revealed significant alterations in the metabolites, including amino acids, biogenic amines, glycerophospholipids, and sphingolipids, caused by the impairment of glycolysis and N-linked glycosylation.
31533338	9	67	theme	SCC15	1540:1544	arg1	cells					1546:1550	SCC15 cells	1540:1550	SCC15 cells induced by 2DG	1540:1565	These observations suggest that alterations of these metabolites may be responsible for the phenotypic and metabolic changes in SCC15 cells induced by 2DG.
31533338	5	68	theme	lactate	757:763	arg1	level					765:769	lactate level	757:769	lactate level	757:769	In this study, we found that 2DG more effectively reduced glucose consumption and lactate level in SCC15 cells than in SCC4 cells, which are less dependent on glycolysis.
31533338	1	69	theme	prominent	146:154	arg1	hallmarks					156:164	the most prominent hallmarks	137:164	the most prominent hallmarks of cancer cells	137:180	One of the most prominent hallmarks of cancer cells is their dependency on the glycolytic pathway for energy production.
31533338	0	70	theme	Met	125:127	arg1	N-Glycosylation					98:112	N-Glycosylation	98:112	N-Glycosylation of Axl and Met	98:127	2-Deoxy-d-Glucose-Induced Metabolic Alteration in Human Oral Squamous SCC15 Cells: Involvement of N-Glycosylation of Axl and Met.
31533338	6	71	gly	glycosylation	884:896	arg1	Axl					929:931	Axl	929:931	Axl	929:931	Coincidentally, 2DG impaired N-linked glycosylation of the key oncogenic receptors Axl and Met in SCC15 cells, thereby reducing the cell viability and colony formation ability.
31533338	6	71	gly	glycosylation	884:896	arg1	receptors					919:927	the key oncogenic receptors	901:927	the key oncogenic receptors Axl and Met	901:939	Coincidentally, 2DG impaired N-linked glycosylation of the key oncogenic receptors Axl and Met in SCC15 cells, thereby reducing the cell viability and colony formation ability.
31533338	6	71	gly	glycosylation	884:896	arg1	Met					937:939	Met	937:939	Met	937:939	Coincidentally, 2DG impaired N-linked glycosylation of the key oncogenic receptors Axl and Met in SCC15 cells, thereby reducing the cell viability and colony formation ability.
31533338	6	71	gly	glycosylation	884:896	arg1	cells					950:954	SCC15 cells	944:954	SCC15 cells	944:954	Coincidentally, 2DG impaired N-linked glycosylation of the key oncogenic receptors Axl and Met in SCC15 cells, thereby reducing the cell viability and colony formation ability.
31533338	11	72	theme	metabolites	1776:1786	arg1	roles					1753:1757	the roles	1749:1757	the roles of these altered metabolites	1749:1786	Further studies are needed to elucidate the roles of these altered metabolites to provide novel therapeutic targets for treating human oral cancer.
31533338	7	73	link	N-linked	1064:1071	arg1	glycosylation					1073:1085	N-linked glycosylation	1064:1085	N-linked glycosylation	1064:1085	The impaired processes of glycolysis and N-linked glycosylation were restored by exogenous addition of pyruvate and mannose, respectively.
31533338	0	74	theme	Metabolic	26:34	arg1	Alteration					36:45	2-Deoxy-d-Glucose-Induced Metabolic Alteration	0:45	2-Deoxy-d-Glucose-Induced Metabolic Alteration in Human Oral Squamous SCC15 Cells: Involvement of N-Glycosylation of Axl and Met.	0:128	2-Deoxy-d-Glucose-Induced Metabolic Alteration in Human Oral Squamous SCC15 Cells: Involvement of N-Glycosylation of Axl and Met.
31533338	0	75	gly	N-Glycosylation	98:112	arg1	Met					125:127	Met	125:127	Met	125:127	2-Deoxy-d-Glucose-Induced Metabolic Alteration in Human Oral Squamous SCC15 Cells: Involvement of N-Glycosylation of Axl and Met.
31533338	0	75	gly	N-Glycosylation	98:112	arg1	Axl					117:119	Axl	117:119	Axl	117:119	2-Deoxy-d-Glucose-Induced Metabolic Alteration in Human Oral Squamous SCC15 Cells: Involvement of N-Glycosylation of Axl and Met.
31533338	4	76	theme	metabolic	551:559	arg1	alteration					561:570	metabolic alteration	551:570	metabolic alteration by 2DG	551:577	To further understand the overall effect of and metabolic alteration by 2DG, we performed biochemical and metabolomics analyses on oral squamous cell carcinoma cell lines.
31533338	5	77	theme	SCC4	794:797	arg1	cells					799:803	SCC4 cells	794:803	SCC4 cells	794:803	In this study, we found that 2DG more effectively reduced glucose consumption and lactate level in SCC15 cells than in SCC4 cells, which are less dependent on glycolysis.
31533338	11	78	theme	Further	1709:1715	arg1	studies					1717:1723	Further studies	1709:1723	Further studies	1709:1723	Further studies are needed to elucidate the roles of these altered metabolites to provide novel therapeutic targets for treating human oral cancer.
31533338	3	79	theme	other	451:455	arg1	glycosylation					488:500	glycosylation	488:500	glycosylation	488:500	Moreover, 2DG has been reported to interfere with other biological processes including glycosylation.
31533338	3	79	theme	other	451:455	arg1	processes					468:476	other biological processes	451:476	other biological processes including glycosylation	451:500	Moreover, 2DG has been reported to interfere with other biological processes including glycosylation.
31533338	0	80	theme	Oral	56:59	arg1	Cells					76:80	Human Oral Squamous SCC15 Cells	50:80	Human Oral Squamous SCC15 Cells	50:80	2-Deoxy-d-Glucose-Induced Metabolic Alteration in Human Oral Squamous SCC15 Cells: Involvement of N-Glycosylation of Axl and Met.
31533338	4	81	theme	overall	529:535	arg1	effect					537:542	the overall effect	525:542	the overall effect of	525:545	To further understand the overall effect of and metabolic alteration by 2DG, we performed biochemical and metabolomics analyses on oral squamous cell carcinoma cell lines.
31533338	8	82	theme	glycosylation	1397:1409	arg1	impairment					1359:1368	the impairment	1355:1368	the impairment of glycolysis and N-linked glycosylation	1355:1409	Additionally, our targeted metabolomics analysis revealed significant alterations in the metabolites, including amino acids, biogenic amines, glycerophospholipids, and sphingolipids, caused by the impairment of glycolysis and N-linked glycosylation.
31533338	0	83	theme	Axl	117:119	arg1	N-Glycosylation					98:112	N-Glycosylation	98:112	N-Glycosylation of Axl and Met	98:127	2-Deoxy-d-Glucose-Induced Metabolic Alteration in Human Oral Squamous SCC15 Cells: Involvement of N-Glycosylation of Axl and Met.
31533338	0	84	theme	SCC15	70:74	arg1	Cells					76:80	Human Oral Squamous SCC15 Cells	50:80	Human Oral Squamous SCC15 Cells	50:80	2-Deoxy-d-Glucose-Induced Metabolic Alteration in Human Oral Squamous SCC15 Cells: Involvement of N-Glycosylation of Axl and Met.
31533338	10	85	from	maintenance	1660:1670	arg1	cells					1702:1706	SCC15 cells	1696:1706	SCC15 cells	1696:1706	Moreover, our data suggest that N-linked glycosylation of Axl and Met may contribute to the maintenance of cancer properties in SCC15 cells.
31533338	6	86	from	glycosylation	884:896	arg1	cells					950:954	SCC15 cells	944:954	SCC15 cells	944:954	Coincidentally, 2DG impaired N-linked glycosylation of the key oncogenic receptors Axl and Met in SCC15 cells, thereby reducing the cell viability and colony formation ability.
31533338	1	87	theme	energy	232:237	arg1	production					239:248	energy production	232:248	energy production	232:248	One of the most prominent hallmarks of cancer cells is their dependency on the glycolytic pathway for energy production.
29428810	4	0	theme	concentrated	754:765	arg1	solution					789:796	a concentrated pluronic F127 aqueous solution	752:796	a concentrated pluronic F127 aqueous solution	752:796	EXPERIMENTS Chitosan, montmorillonite and chitosan-montmorillonite nanocomposites were added on a concentrated pluronic F127 aqueous solution.
29428810	3	1	theme	temperature-sensitive	624:644	arg1	vehicles					646:653	bio-based temperature-sensitive vehicles	614:653	bio-based temperature-sensitive vehicles	614:653	The combined addition of chitosan and montmorillonite opens the possibility to join the properties of the single constituents to formulate bio-based temperature-sensitive vehicles.
29428810	1	2	theme	gel-like	163:170	arg1	behavior					172:179	The thermoreversible gel-like behavior	142:179	HYPOTHESIS The thermoreversible gel-like behavior of pluronics	131:192	HYPOTHESIS The thermoreversible gel-like behavior of pluronics can be affected by the presence of drugs or cosolvents.
29428810	5	3	theme	differential	847:858	arg1	DSC					882:884	DSC	882:884	DSC	882:884	The pluronic-based systems were investigated by differential scanning calorimetry (DSC), X-ray diffraction (XRD), rheology and Fourier transform infrared attenuated total reflection spectroscopy (FTIR-ATR).
29428810	5	3	theme	differential	847:858	arg1	calorimetry					869:879	differential scanning calorimetry	847:879	differential scanning calorimetry (DSC)	847:885	The pluronic-based systems were investigated by differential scanning calorimetry (DSC), X-ray diffraction (XRD), rheology and Fourier transform infrared attenuated total reflection spectroscopy (FTIR-ATR).
29428810	7	4	theme	FTIR-ATR	1200:1207	arg1	spectroscopy					1209:1220	FTIR-ATR spectroscopy	1200:1220	FTIR-ATR spectroscopy	1200:1220	Then, FTIR-ATR spectroscopy was applied to analyze different vibrational modes in order to evidence differences in the conformational arrangements of the micelles.
29428810	5	5	theme	total	964:968	arg1	FTIR-ATR					995:1002	FTIR-ATR	995:1002	FTIR-ATR	995:1002	The pluronic-based systems were investigated by differential scanning calorimetry (DSC), X-ray diffraction (XRD), rheology and Fourier transform infrared attenuated total reflection spectroscopy (FTIR-ATR).
29428810	5	5	theme	total	964:968	arg1	spectroscopy					981:992	attenuated total reflection spectroscopy	953:992	attenuated total reflection spectroscopy (FTIR-ATR)	953:1003	The pluronic-based systems were investigated by differential scanning calorimetry (DSC), X-ray diffraction (XRD), rheology and Fourier transform infrared attenuated total reflection spectroscopy (FTIR-ATR).
29428810	2	6	theme	water	333:337	arg1	dispersion					339:348	a pluronic water dispersion	322:348	a pluronic water dispersion	322:348	So far, the effects of polysaccharides and of clays singularly added on a pluronic water dispersion were investigated and the gelation and viscoelastic properties tuned by properly varying the concentration of the additives.
29428810	0	7	from	Effect	0:5	arg1	solution					82:89	concentrated pluronic F127 solution	55:89	concentrated pluronic F127 solution	55:89	Effect of intercalated chitosan/clay nanostructures on concentrated pluronic F127 solution: A FTIR-ATR, DSC and rheological study.
29428810	2	8	theme	polysaccharides	273:287	arg1	effects					262:268	the effects	258:268	the effects of polysaccharides and of clays singularly added on a pluronic water dispersion	258:348	So far, the effects of polysaccharides and of clays singularly added on a pluronic water dispersion were investigated and the gelation and viscoelastic properties tuned by properly varying the concentration of the additives.
29428810	8	9	theme	pluronic	1491:1498	arg1	arrangements					1475:1486	the micellar arrangements	1462:1486	the micellar arrangements of pluronic	1462:1498	FINDINGS The experiments evidenced that the chitosan/clay nanocomposites have a destructuring effect on the micellar arrangements of pluronic and that the chaotropic effect by chitosan dominates over the ordering effect by the clay.
29428810	7	10	theme	evidence	1285:1292	arg1	differences					1294:1304	evidence differences	1285:1304	evidence differences in the conformational arrangements of the micelles	1285:1355	Then, FTIR-ATR spectroscopy was applied to analyze different vibrational modes in order to evidence differences in the conformational arrangements of the micelles.
29428810	1	11	dep	HYPOTHESIS	131:140	arg1	behavior					172:179	The thermoreversible gel-like behavior	142:179	HYPOTHESIS The thermoreversible gel-like behavior of pluronics	131:192	HYPOTHESIS The thermoreversible gel-like behavior of pluronics can be affected by the presence of drugs or cosolvents.
29428810	3	12	theme	combined	479:486	arg1	addition					488:495	The combined addition	475:495	The combined addition of chitosan and montmorillonite	475:527	The combined addition of chitosan and montmorillonite opens the possibility to join the properties of the single constituents to formulate bio-based temperature-sensitive vehicles.
29428810	5	13	theme	pluronic-based	803:816	arg1	systems					818:824	The pluronic-based systems	799:824	The pluronic-based systems	799:824	The pluronic-based systems were investigated by differential scanning calorimetry (DSC), X-ray diffraction (XRD), rheology and Fourier transform infrared attenuated total reflection spectroscopy (FTIR-ATR).
29428810	4	14	theme	F127	776:779	arg1	solution					789:796	a concentrated pluronic F127 aqueous solution	752:796	a concentrated pluronic F127 aqueous solution	752:796	EXPERIMENTS Chitosan, montmorillonite and chitosan-montmorillonite nanocomposites were added on a concentrated pluronic F127 aqueous solution.
29428810	7	15	theme	different	1245:1253	arg1	modes					1267:1271	different vibrational modes	1245:1271	different vibrational modes	1245:1271	Then, FTIR-ATR spectroscopy was applied to analyze different vibrational modes in order to evidence differences in the conformational arrangements of the micelles.
29428810	6	16	theme	elasticity	1154:1163	arg1	terms					1131:1135	terms	1131:1135	terms of the different elasticity of the investigated samples	1131:1191	The gelation and micellization behaviors of pluronic were compared to those of the pluronic-based composites and analyzed in terms of the different elasticity of the investigated samples.
29428810	8	17	theme	micellar	1466:1473	arg1	arrangements					1475:1486	the micellar arrangements	1462:1486	the micellar arrangements of pluronic	1462:1498	FINDINGS The experiments evidenced that the chitosan/clay nanocomposites have a destructuring effect on the micellar arrangements of pluronic and that the chaotropic effect by chitosan dominates over the ordering effect by the clay.
29428810	4	18	theme	chitosan-montmorillonite	698:721	arg1	nanocomposites					723:736	chitosan-montmorillonite nanocomposites	698:736	chitosan-montmorillonite nanocomposites	698:736	EXPERIMENTS Chitosan, montmorillonite and chitosan-montmorillonite nanocomposites were added on a concentrated pluronic F127 aqueous solution.
29428810	8	19	dep	FINDINGS	1358:1365	arg1	evidenced					1383:1391	evidenced	1383:1391	evidenced that the chitosan/clay nanocomposites have a destructuring effect on the micellar arrangements of pluronic and that the chaotropic effect by chitosan dominates over the ordering effect by the clay	1383:1588	FINDINGS The experiments evidenced that the chitosan/clay nanocomposites have a destructuring effect on the micellar arrangements of pluronic and that the chaotropic effect by chitosan dominates over the ordering effect by the clay.
29428810	5	20	theme	scanning	860:867	arg1	DSC					882:884	DSC	882:884	DSC	882:884	The pluronic-based systems were investigated by differential scanning calorimetry (DSC), X-ray diffraction (XRD), rheology and Fourier transform infrared attenuated total reflection spectroscopy (FTIR-ATR).
29428810	5	20	theme	scanning	860:867	arg1	calorimetry					869:879	differential scanning calorimetry	847:879	differential scanning calorimetry (DSC)	847:885	The pluronic-based systems were investigated by differential scanning calorimetry (DSC), X-ray diffraction (XRD), rheology and Fourier transform infrared attenuated total reflection spectroscopy (FTIR-ATR).
29428810	6	21	theme	pluronic-based	1089:1102	arg1	composites					1104:1113	the pluronic-based composites	1085:1113	the pluronic-based composites	1085:1113	The gelation and micellization behaviors of pluronic were compared to those of the pluronic-based composites and analyzed in terms of the different elasticity of the investigated samples.
29428810	1	22	theme	pluronics	184:192	arg1	HYPOTHESIS					131:140	HYPOTHESIS	131:140	HYPOTHESIS The thermoreversible gel-like behavior of pluronics	131:192	HYPOTHESIS The thermoreversible gel-like behavior of pluronics can be affected by the presence of drugs or cosolvents.
29428810	7	23	theme	vibrational	1255:1265	arg1	modes					1267:1271	different vibrational modes	1245:1271	different vibrational modes	1245:1271	Then, FTIR-ATR spectroscopy was applied to analyze different vibrational modes in order to evidence differences in the conformational arrangements of the micelles.
29428810	7	24	theme	micelles	1348:1355	arg1	arrangements					1328:1339	the conformational arrangements	1309:1339	the conformational arrangements of the micelles	1309:1355	Then, FTIR-ATR spectroscopy was applied to analyze different vibrational modes in order to evidence differences in the conformational arrangements of the micelles.
29428810	6	25	theme	pluronic	1050:1057	arg1	behaviors					1037:1045	The gelation and micellization behaviors	1006:1045	The gelation and micellization behaviors of pluronic	1006:1057	The gelation and micellization behaviors of pluronic were compared to those of the pluronic-based composites and analyzed in terms of the different elasticity of the investigated samples.
29428810	4	26	theme	aqueous	781:787	arg1	solution					789:796	a concentrated pluronic F127 aqueous solution	752:796	a concentrated pluronic F127 aqueous solution	752:796	EXPERIMENTS Chitosan, montmorillonite and chitosan-montmorillonite nanocomposites were added on a concentrated pluronic F127 aqueous solution.
29428810	2	27	theme	clays	296:300	arg1	effects					262:268	the effects	258:268	the effects of polysaccharides and of clays singularly added on a pluronic water dispersion	258:348	So far, the effects of polysaccharides and of clays singularly added on a pluronic water dispersion were investigated and the gelation and viscoelastic properties tuned by properly varying the concentration of the additives.
29428810	0	28	theme	chitosan/clay	23:35	arg1	nanostructures					37:50	intercalated chitosan/clay nanostructures	10:50	intercalated chitosan/clay nanostructures	10:50	Effect of intercalated chitosan/clay nanostructures on concentrated pluronic F127 solution: A FTIR-ATR, DSC and rheological study.
29428810	4	29	dep	EXPERIMENTS	656:666	arg1	EXPERIMENTS					656:666	EXPERIMENTS Chitosan, montmorillonite and chitosan-montmorillonite nanocomposites	656:736	EXPERIMENTS Chitosan, montmorillonite and chitosan-montmorillonite nanocomposites	656:736	EXPERIMENTS Chitosan, montmorillonite and chitosan-montmorillonite nanocomposites were added on a concentrated pluronic F127 aqueous solution.
29428810	4	29	dep	EXPERIMENTS	656:666	arg1	montmorillonite					678:692	montmorillonite	678:692	montmorillonite	678:692	EXPERIMENTS Chitosan, montmorillonite and chitosan-montmorillonite nanocomposites were added on a concentrated pluronic F127 aqueous solution.
29428810	4	29	dep	EXPERIMENTS	656:666	arg1	nanocomposites					723:736	chitosan-montmorillonite nanocomposites	698:736	chitosan-montmorillonite nanocomposites	698:736	EXPERIMENTS Chitosan, montmorillonite and chitosan-montmorillonite nanocomposites were added on a concentrated pluronic F127 aqueous solution.
29428810	4	29	dep	EXPERIMENTS	656:666	arg1	Chitosan					668:675	Chitosan	668:675	Chitosan	668:675	EXPERIMENTS Chitosan, montmorillonite and chitosan-montmorillonite nanocomposites were added on a concentrated pluronic F127 aqueous solution.
29428810	0	30	theme	intercalated	10:21	arg1	nanostructures					37:50	intercalated chitosan/clay nanostructures	10:50	intercalated chitosan/clay nanostructures	10:50	Effect of intercalated chitosan/clay nanostructures on concentrated pluronic F127 solution: A FTIR-ATR, DSC and rheological study.
29428810	0	31	theme	rheological	112:122	arg1	study					124:128	rheological study	112:128	rheological study	112:128	Effect of intercalated chitosan/clay nanostructures on concentrated pluronic F127 solution: A FTIR-ATR, DSC and rheological study.
29428810	8	32	theme	ordering	1562:1569	arg1	effect					1571:1576	the ordering effect	1558:1576	the ordering effect by the clay	1558:1588	FINDINGS The experiments evidenced that the chitosan/clay nanocomposites have a destructuring effect on the micellar arrangements of pluronic and that the chaotropic effect by chitosan dominates over the ordering effect by the clay.
29428810	5	33	theme	reflection	970:979	arg1	FTIR-ATR					995:1002	FTIR-ATR	995:1002	FTIR-ATR	995:1002	The pluronic-based systems were investigated by differential scanning calorimetry (DSC), X-ray diffraction (XRD), rheology and Fourier transform infrared attenuated total reflection spectroscopy (FTIR-ATR).
29428810	5	33	theme	reflection	970:979	arg1	spectroscopy					981:992	attenuated total reflection spectroscopy	953:992	attenuated total reflection spectroscopy (FTIR-ATR)	953:1003	The pluronic-based systems were investigated by differential scanning calorimetry (DSC), X-ray diffraction (XRD), rheology and Fourier transform infrared attenuated total reflection spectroscopy (FTIR-ATR).
29428810	6	34	theme	samples	1185:1191	arg1	elasticity					1154:1163	the different elasticity	1140:1163	the different elasticity of the investigated samples	1140:1191	The gelation and micellization behaviors of pluronic were compared to those of the pluronic-based composites and analyzed in terms of the different elasticity of the investigated samples.
29428810	3	35	theme	chitosan	500:507	arg1	addition					488:495	The combined addition	475:495	The combined addition of chitosan and montmorillonite	475:527	The combined addition of chitosan and montmorillonite opens the possibility to join the properties of the single constituents to formulate bio-based temperature-sensitive vehicles.
29428810	6	36	theme	micellization	1023:1035	arg1	behaviors					1037:1045	The gelation and micellization behaviors	1006:1045	The gelation and micellization behaviors of pluronic	1006:1057	The gelation and micellization behaviors of pluronic were compared to those of the pluronic-based composites and analyzed in terms of the different elasticity of the investigated samples.
29428810	5	37	dep	Fourier	926:932	arg1	transform					934:942	transform	934:942	transform infrared attenuated total reflection spectroscopy (FTIR-ATR)	934:1003	The pluronic-based systems were investigated by differential scanning calorimetry (DSC), X-ray diffraction (XRD), rheology and Fourier transform infrared attenuated total reflection spectroscopy (FTIR-ATR).
29428810	2	38	theme	additives	464:472	arg1	concentration					443:455	the concentration	439:455	the concentration of the additives	439:472	So far, the effects of polysaccharides and of clays singularly added on a pluronic water dispersion were investigated and the gelation and viscoelastic properties tuned by properly varying the concentration of the additives.
29428810	0	39	theme	nanostructures	37:50	arg1	Effect					0:5	Effect	0:5	Effect of intercalated chitosan/clay nanostructures on concentrated pluronic F127 solution: A FTIR-ATR, DSC and rheological study.	0:129	Effect of intercalated chitosan/clay nanostructures on concentrated pluronic F127 solution: A FTIR-ATR, DSC and rheological study.
29428810	5	40	theme	X-ray	888:892	arg1	XRD					907:909	XRD	907:909	XRD	907:909	The pluronic-based systems were investigated by differential scanning calorimetry (DSC), X-ray diffraction (XRD), rheology and Fourier transform infrared attenuated total reflection spectroscopy (FTIR-ATR).
29428810	5	40	theme	X-ray	888:892	arg1	diffraction					894:904	X-ray diffraction	888:904	X-ray diffraction (XRD)	888:910	The pluronic-based systems were investigated by differential scanning calorimetry (DSC), X-ray diffraction (XRD), rheology and Fourier transform infrared attenuated total reflection spectroscopy (FTIR-ATR).
29428810	2	41	theme	viscoelastic	389:400	arg1	properties					402:411	viscoelastic properties	389:411	viscoelastic properties	389:411	So far, the effects of polysaccharides and of clays singularly added on a pluronic water dispersion were investigated and the gelation and viscoelastic properties tuned by properly varying the concentration of the additives.
29428810	0	42	theme	pluronic	68:75	arg1	solution					82:89	concentrated pluronic F127 solution	55:89	concentrated pluronic F127 solution	55:89	Effect of intercalated chitosan/clay nanostructures on concentrated pluronic F127 solution: A FTIR-ATR, DSC and rheological study.
29428810	6	43	theme	gelation	1010:1017	arg1	behaviors					1037:1045	The gelation and micellization behaviors	1006:1045	The gelation and micellization behaviors of pluronic	1006:1057	The gelation and micellization behaviors of pluronic were compared to those of the pluronic-based composites and analyzed in terms of the different elasticity of the investigated samples.
29428810	7	44	theme	conformational	1313:1326	arg1	arrangements					1328:1339	the conformational arrangements	1309:1339	the conformational arrangements of the micelles	1309:1355	Then, FTIR-ATR spectroscopy was applied to analyze different vibrational modes in order to evidence differences in the conformational arrangements of the micelles.
29428810	3	45	theme	single	581:586	arg1	constituents					588:599	the single constituents	577:599	the single constituents	577:599	The combined addition of chitosan and montmorillonite opens the possibility to join the properties of the single constituents to formulate bio-based temperature-sensitive vehicles.
29428810	6	46	theme	investigated	1172:1183	arg1	samples					1185:1191	the investigated samples	1168:1191	the investigated samples	1168:1191	The gelation and micellization behaviors of pluronic were compared to those of the pluronic-based composites and analyzed in terms of the different elasticity of the investigated samples.
29428810	2	47	theme	pluronic	324:331	arg1	dispersion					339:348	a pluronic water dispersion	322:348	a pluronic water dispersion	322:348	So far, the effects of polysaccharides and of clays singularly added on a pluronic water dispersion were investigated and the gelation and viscoelastic properties tuned by properly varying the concentration of the additives.
29428810	0	48	theme	concentrated	55:66	arg1	solution					82:89	concentrated pluronic F127 solution	55:89	concentrated pluronic F127 solution	55:89	Effect of intercalated chitosan/clay nanostructures on concentrated pluronic F127 solution: A FTIR-ATR, DSC and rheological study.
29428810	7	49	from	differences	1294:1304	arg1	arrangements					1328:1339	the conformational arrangements	1309:1339	the conformational arrangements of the micelles	1309:1355	Then, FTIR-ATR spectroscopy was applied to analyze different vibrational modes in order to evidence differences in the conformational arrangements of the micelles.
29428810	3	50	theme	constituents	588:599	arg1	properties					563:572	the properties	559:572	the properties of the single constituents	559:599	The combined addition of chitosan and montmorillonite opens the possibility to join the properties of the single constituents to formulate bio-based temperature-sensitive vehicles.
29428810	4	51	theme	pluronic	767:774	arg1	solution					789:796	a concentrated pluronic F127 aqueous solution	752:796	a concentrated pluronic F127 aqueous solution	752:796	EXPERIMENTS Chitosan, montmorillonite and chitosan-montmorillonite nanocomposites were added on a concentrated pluronic F127 aqueous solution.
29428810	2	52	from	effects	262:268	arg1	dispersion					339:348	a pluronic water dispersion	322:348	a pluronic water dispersion	322:348	So far, the effects of polysaccharides and of clays singularly added on a pluronic water dispersion were investigated and the gelation and viscoelastic properties tuned by properly varying the concentration of the additives.
29428810	2	53	dep	gelation	376:383	arg1	the					372:374	the	372:374	the	372:374	So far, the effects of polysaccharides and of clays singularly added on a pluronic water dispersion were investigated and the gelation and viscoelastic properties tuned by properly varying the concentration of the additives.
29428810	8	54	contain	have	1431:1434	arg2	effect					1452:1457	a destructuring effect	1436:1457	a destructuring effect	1436:1457	FINDINGS The experiments evidenced that the chitosan/clay nanocomposites have a destructuring effect on the micellar arrangements of pluronic and that the chaotropic effect by chitosan dominates over the ordering effect by the clay.
29428810	8	54	contain	have	1431:1434	arg1	nanocomposites					1416:1429	the chitosan/clay nanocomposites	1398:1429	the chitosan/clay nanocomposites	1398:1429	FINDINGS The experiments evidenced that the chitosan/clay nanocomposites have a destructuring effect on the micellar arrangements of pluronic and that the chaotropic effect by chitosan dominates over the ordering effect by the clay.
29428810	5	55	dep	transform	934:942	arg1	infrared					944:951	infrared	944:951	transform infrared attenuated total reflection spectroscopy (FTIR-ATR)	934:1003	The pluronic-based systems were investigated by differential scanning calorimetry (DSC), X-ray diffraction (XRD), rheology and Fourier transform infrared attenuated total reflection spectroscopy (FTIR-ATR).
29428810	8	56	theme	chitosan/clay	1402:1414	arg1	nanocomposites					1416:1429	the chitosan/clay nanocomposites	1398:1429	the chitosan/clay nanocomposites	1398:1429	FINDINGS The experiments evidenced that the chitosan/clay nanocomposites have a destructuring effect on the micellar arrangements of pluronic and that the chaotropic effect by chitosan dominates over the ordering effect by the clay.
29428810	2	57	theme	added	313:317	arg1	clays					296:300	clays	296:300	clays singularly added	296:317	So far, the effects of polysaccharides and of clays singularly added on a pluronic water dispersion were investigated and the gelation and viscoelastic properties tuned by properly varying the concentration of the additives.
29428810	8	58	theme	destructuring	1438:1450	arg1	effect					1452:1457	a destructuring effect	1436:1457	a destructuring effect	1436:1457	FINDINGS The experiments evidenced that the chitosan/clay nanocomposites have a destructuring effect on the micellar arrangements of pluronic and that the chaotropic effect by chitosan dominates over the ordering effect by the clay.
29428810	1	59	theme	drugs	229:233	arg1	presence					217:224	the presence	213:224	the presence of drugs or cosolvents	213:247	HYPOTHESIS The thermoreversible gel-like behavior of pluronics can be affected by the presence of drugs or cosolvents.
29428810	5	60	theme	attenuated	953:962	arg1	FTIR-ATR					995:1002	FTIR-ATR	995:1002	FTIR-ATR	995:1002	The pluronic-based systems were investigated by differential scanning calorimetry (DSC), X-ray diffraction (XRD), rheology and Fourier transform infrared attenuated total reflection spectroscopy (FTIR-ATR).
29428810	5	60	theme	attenuated	953:962	arg1	spectroscopy					981:992	attenuated total reflection spectroscopy	953:992	attenuated total reflection spectroscopy (FTIR-ATR)	953:1003	The pluronic-based systems were investigated by differential scanning calorimetry (DSC), X-ray diffraction (XRD), rheology and Fourier transform infrared attenuated total reflection spectroscopy (FTIR-ATR).
29428810	3	61	theme	bio-based	614:622	arg1	vehicles					646:653	bio-based temperature-sensitive vehicles	614:653	bio-based temperature-sensitive vehicles	614:653	The combined addition of chitosan and montmorillonite opens the possibility to join the properties of the single constituents to formulate bio-based temperature-sensitive vehicles.
29428810	3	62	theme	montmorillonite	513:527	arg1	addition					488:495	The combined addition	475:495	The combined addition of chitosan and montmorillonite	475:527	The combined addition of chitosan and montmorillonite opens the possibility to join the properties of the single constituents to formulate bio-based temperature-sensitive vehicles.
29428810	6	63	theme	different	1144:1152	arg1	elasticity					1154:1163	the different elasticity	1140:1163	the different elasticity of the investigated samples	1140:1191	The gelation and micellization behaviors of pluronic were compared to those of the pluronic-based composites and analyzed in terms of the different elasticity of the investigated samples.
29428810	0	64	theme	F127	77:80	arg1	solution					82:89	concentrated pluronic F127 solution	55:89	concentrated pluronic F127 solution	55:89	Effect of intercalated chitosan/clay nanostructures on concentrated pluronic F127 solution: A FTIR-ATR, DSC and rheological study.
29428810	0	65	dep	Effect	0:5	arg1	DSC					104:106	DSC	104:106	DSC	104:106	Effect of intercalated chitosan/clay nanostructures on concentrated pluronic F127 solution: A FTIR-ATR, DSC and rheological study.
29428810	0	65	dep	Effect	0:5	arg1	study					124:128	rheological study	112:128	rheological study	112:128	Effect of intercalated chitosan/clay nanostructures on concentrated pluronic F127 solution: A FTIR-ATR, DSC and rheological study.
29428810	0	65	dep	Effect	0:5	arg1	FTIR-ATR					94:101	FTIR-ATR	94:101	FTIR-ATR	94:101	Effect of intercalated chitosan/clay nanostructures on concentrated pluronic F127 solution: A FTIR-ATR, DSC and rheological study.
29428810	1	66	theme	thermoreversible	146:161	arg1	behavior					172:179	The thermoreversible gel-like behavior	142:179	HYPOTHESIS The thermoreversible gel-like behavior of pluronics	131:192	HYPOTHESIS The thermoreversible gel-like behavior of pluronics can be affected by the presence of drugs or cosolvents.
29428810	8	67	theme	chaotropic	1513:1522	arg1	effect					1524:1529	the chaotropic effect	1509:1529	the chaotropic effect by chitosan	1509:1541	FINDINGS The experiments evidenced that the chitosan/clay nanocomposites have a destructuring effect on the micellar arrangements of pluronic and that the chaotropic effect by chitosan dominates over the ordering effect by the clay.
29428810	1	68	theme	cosolvents	238:247	arg1	presence					217:224	the presence	213:224	the presence of drugs or cosolvents	213:247	HYPOTHESIS The thermoreversible gel-like behavior of pluronics can be affected by the presence of drugs or cosolvents.
31837260	4	0	theme	sequencing	545:554	arg1	methods					556:562	the 16S rRNA and metagenomic sequencing methods	516:562	the 16S rRNA and metagenomic sequencing methods	516:562	Here, using the 16S rRNA and metagenomic sequencing methods, we characterized the microbial phenotypes of 16 female cynomolgus macaques from three age groups (young, adult and old).
31837260	0	1	from	changes	12:18	arg1	function					49:56	function	49:56	function	49:56	Age-related changes in microbial composition and function in cynomolgus macaques.
31837260	0	1	from	changes	12:18	arg1	macaques					72:79	cynomolgus macaques	61:79	cynomolgus macaques	61:79	Age-related changes in microbial composition and function in cynomolgus macaques.
31837260	0	1	from	changes	12:18	arg1	composition					33:43	microbial composition	23:43	microbial composition	23:43	Age-related changes in microbial composition and function in cynomolgus macaques.
31837260	6	2	theme	relative	805:812	arg1	abundances					814:823	the relative abundances	801:823	the relative abundances of Veillonellaceae, Coriobacteriaceae and Succinivibrionaceae were significantly increased, Ruminococcaceae and Rikenellaceae	801:949	With increased age, the relative abundances of Veillonellaceae, Coriobacteriaceae and Succinivibrionaceae were significantly increased, Ruminococcaceae and Rikenellaceae were significantly decreased at the family level.
31837260	9	3	dep	composition	1335:1345	arg1	the					1331:1333	the	1331:1333	the	1331:1333	In conclusion, we characterized the composition and function of the gut microbiota at different ages, and our findings provide a new entry point for understanding the effects of age on the human body.
31837260	4	4	theme	metagenomic	533:543	arg1	sequencing					545:554	metagenomic sequencing	533:554	metagenomic sequencing	533:554	Here, using the 16S rRNA and metagenomic sequencing methods, we characterized the microbial phenotypes of 16 female cynomolgus macaques from three age groups (young, adult and old).
31837260	6	5	theme	Succinivibrionaceae	867:885	arg1	abundances					814:823	the relative abundances	801:823	the relative abundances of Veillonellaceae, Coriobacteriaceae and Succinivibrionaceae were significantly increased, Ruminococcaceae and Rikenellaceae	801:949	With increased age, the relative abundances of Veillonellaceae, Coriobacteriaceae and Succinivibrionaceae were significantly increased, Ruminococcaceae and Rikenellaceae were significantly decreased at the family level.
31837260	7	6	theme	purine	1125:1130	arg1	metabolism					1132:1141	purine metabolism	1125:1141	purine metabolism	1125:1141	Functional enrichment showed that genes that differed among the three groups were mainly involved in arginine biosynthesis, purine metabolism and microbial polysaccharides metabolism.
31837260	6	7	theme	family	987:992	arg1	level					994:998	the family level	983:998	the family level	983:998	With increased age, the relative abundances of Veillonellaceae, Coriobacteriaceae and Succinivibrionaceae were significantly increased, Ruminococcaceae and Rikenellaceae were significantly decreased at the family level.
31837260	7	8	theme	arginine	1102:1109	arg1	biosynthesis					1111:1122	arginine biosynthesis	1102:1122	arginine biosynthesis	1102:1122	Functional enrichment showed that genes that differed among the three groups were mainly involved in arginine biosynthesis, purine metabolism and microbial polysaccharides metabolism.
31837260	4	9	dep	groups	655:660	arg1	adult					670:674	adult	670:674	adult	670:674	Here, using the 16S rRNA and metagenomic sequencing methods, we characterized the microbial phenotypes of 16 female cynomolgus macaques from three age groups (young, adult and old).
31837260	4	9	dep	groups	655:660	arg1	young					663:667	young	663:667	young	663:667	Here, using the 16S rRNA and metagenomic sequencing methods, we characterized the microbial phenotypes of 16 female cynomolgus macaques from three age groups (young, adult and old).
31837260	4	9	dep	groups	655:660	arg1	old					680:682	old	680:682	old	680:682	Here, using the 16S rRNA and metagenomic sequencing methods, we characterized the microbial phenotypes of 16 female cynomolgus macaques from three age groups (young, adult and old).
31837260	4	10	theme	macaques	631:638	arg1	phenotypes					596:605	the microbial phenotypes	582:605	the microbial phenotypes of 16 female cynomolgus macaques	582:638	Here, using the 16S rRNA and metagenomic sequencing methods, we characterized the microbial phenotypes of 16 female cynomolgus macaques from three age groups (young, adult and old).
31837260	2	11	theme	Recent	146:151	arg1	studies					153:159	Recent studies	146:159	Recent studies	146:159	Recent studies have shown that age may affect the composition and function of the gut microbiota, but the underlying mechanisms remain largely unknown.
31837260	9	12	from	ages	1395:1398	arg1	composition					1335:1345	composition	1335:1345	composition	1335:1345	In conclusion, we characterized the composition and function of the gut microbiota at different ages, and our findings provide a new entry point for understanding the effects of age on the human body.
31837260	9	12	from	ages	1395:1398	arg1	function					1351:1358	function	1351:1358	function	1351:1358	In conclusion, we characterized the composition and function of the gut microbiota at different ages, and our findings provide a new entry point for understanding the effects of age on the human body.
31837260	2	13	theme	underlying	252:261	arg1	mechanisms					263:272	the underlying mechanisms	248:272	the underlying mechanisms	248:272	Recent studies have shown that age may affect the composition and function of the gut microbiota, but the underlying mechanisms remain largely unknown.
31837260	4	14	theme	cynomolgus	620:629	arg1	macaques					631:638	16 female cynomolgus macaques	610:638	16 female cynomolgus macaques	610:638	Here, using the 16S rRNA and metagenomic sequencing methods, we characterized the microbial phenotypes of 16 female cynomolgus macaques from three age groups (young, adult and old).
31837260	3	15	theme	gut	370:372	arg1	microbiota					374:383	the gut microbiota	366:383	the gut microbiota	366:383	Non-human primates are an ideal model for uncovering how age shapes the gut microbiota, as their microbial composition is highly similar to that of humans and is not easily affected by confounding factors.
31837260	9	16	theme	gut	1367:1369	arg1	microbiota					1371:1380	the gut microbiota	1363:1380	the gut microbiota	1363:1380	In conclusion, we characterized the composition and function of the gut microbiota at different ages, and our findings provide a new entry point for understanding the effects of age on the human body.
31837260	9	17	theme	microbiota	1371:1380	arg1	composition					1335:1345	composition	1335:1345	composition	1335:1345	In conclusion, we characterized the composition and function of the gut microbiota at different ages, and our findings provide a new entry point for understanding the effects of age on the human body.
31837260	9	17	theme	microbiota	1371:1380	arg1	function					1351:1358	function	1351:1358	function	1351:1358	In conclusion, we characterized the composition and function of the gut microbiota at different ages, and our findings provide a new entry point for understanding the effects of age on the human body.
31837260	9	18	theme	age	1477:1479	arg1	effects					1466:1472	the effects	1462:1472	the effects of age on the human body	1462:1497	In conclusion, we characterized the composition and function of the gut microbiota at different ages, and our findings provide a new entry point for understanding the effects of age on the human body.
31837260	0	19	theme	Age-related	0:10	arg1	changes					12:18	Age-related changes	0:18	Age-related changes in microbial composition and function in cynomolgus macaques	0:79	Age-related changes in microbial composition and function in cynomolgus macaques.
31837260	4	20	theme	16S	520:522	arg1	rRNA					524:527	16S rRNA	520:527	16S rRNA	520:527	Here, using the 16S rRNA and metagenomic sequencing methods, we characterized the microbial phenotypes of 16 female cynomolgus macaques from three age groups (young, adult and old).
31837260	9	21	theme	entry	1432:1436	arg1	point					1438:1442	a new entry point	1426:1442	a new entry point for understanding the effects of age on the human body	1426:1497	In conclusion, we characterized the composition and function of the gut microbiota at different ages, and our findings provide a new entry point for understanding the effects of age on the human body.
31837260	0	22	theme	microbial	23:31	arg1	composition					33:43	microbial composition	23:43	microbial composition	23:43	Age-related changes in microbial composition and function in cynomolgus macaques.
31837260	8	23	theme	degrading	1235:1243	arg1	activities					1245:1254	polysaccharide degrading activities	1220:1254	polysaccharide degrading activities	1220:1254	Moreover, CAZymes corresponding to polysaccharide degrading activities were also observed among the three groups.
31837260	9	24	theme	different	1385:1393	arg1	ages					1395:1398	different ages	1385:1398	different ages	1385:1398	In conclusion, we characterized the composition and function of the gut microbiota at different ages, and our findings provide a new entry point for understanding the effects of age on the human body.
31837260	6	25	theme	Veillonellaceae	828:842	arg1	abundances					814:823	the relative abundances	801:823	the relative abundances of Veillonellaceae, Coriobacteriaceae and Succinivibrionaceae were significantly increased, Ruminococcaceae and Rikenellaceae	801:949	With increased age, the relative abundances of Veillonellaceae, Coriobacteriaceae and Succinivibrionaceae were significantly increased, Ruminococcaceae and Rikenellaceae were significantly decreased at the family level.
31837260	1	26	theme	human	111:115	arg1	physiology					117:126	human physiology	111:126	human physiology	111:126	Age can significantly affect human physiology and disease risk.
31837260	9	27	theme	human	1488:1492	arg1	body					1494:1497	the human body	1484:1497	the human body	1484:1497	In conclusion, we characterized the composition and function of the gut microbiota at different ages, and our findings provide a new entry point for understanding the effects of age on the human body.
31837260	5	28	theme	microbial	735:743	arg1	composition					745:755	microbial composition	735:755	microbial composition	735:755	Our findings revealed significant differences in microbial composition among the three groups.
31837260	4	29	theme	rRNA	524:527	arg1	methods					556:562	the 16S rRNA and metagenomic sequencing methods	516:562	the 16S rRNA and metagenomic sequencing methods	516:562	Here, using the 16S rRNA and metagenomic sequencing methods, we characterized the microbial phenotypes of 16 female cynomolgus macaques from three age groups (young, adult and old).
31837260	2	30	theme	gut	228:230	arg1	microbiota					232:241	the gut microbiota	224:241	the gut microbiota	224:241	Recent studies have shown that age may affect the composition and function of the gut microbiota, but the underlying mechanisms remain largely unknown.
31837260	4	31	theme	female	613:618	arg1	macaques					631:638	16 female cynomolgus macaques	610:638	16 female cynomolgus macaques	610:638	Here, using the 16S rRNA and metagenomic sequencing methods, we characterized the microbial phenotypes of 16 female cynomolgus macaques from three age groups (young, adult and old).
31837260	3	32	theme	microbial	395:403	arg1	composition					405:415	their microbial composition	389:415	their microbial composition	389:415	Non-human primates are an ideal model for uncovering how age shapes the gut microbiota, as their microbial composition is highly similar to that of humans and is not easily affected by confounding factors.
31837260	9	33	theme	new	1428:1430	arg1	point					1438:1442	a new entry point	1426:1442	a new entry point for understanding the effects of age on the human body	1426:1497	In conclusion, we characterized the composition and function of the gut microbiota at different ages, and our findings provide a new entry point for understanding the effects of age on the human body.
31837260	8	34	theme	polysaccharide	1220:1233	arg1	activities					1245:1254	polysaccharide degrading activities	1220:1254	polysaccharide degrading activities	1220:1254	Moreover, CAZymes corresponding to polysaccharide degrading activities were also observed among the three groups.
31837260	7	35	theme	microbial	1147:1155	arg1	metabolism					1173:1182	microbial polysaccharides metabolism	1147:1182	microbial polysaccharides metabolism	1147:1182	Functional enrichment showed that genes that differed among the three groups were mainly involved in arginine biosynthesis, purine metabolism and microbial polysaccharides metabolism.
31837260	6	36	theme	Rikenellaceae	937:949	arg1	abundances					814:823	the relative abundances	801:823	the relative abundances of Veillonellaceae, Coriobacteriaceae and Succinivibrionaceae were significantly increased, Ruminococcaceae and Rikenellaceae	801:949	With increased age, the relative abundances of Veillonellaceae, Coriobacteriaceae and Succinivibrionaceae were significantly increased, Ruminococcaceae and Rikenellaceae were significantly decreased at the family level.
31837260	3	37	theme	Non-human	298:306	arg1	model					330:334	an ideal model	321:334	an ideal model for uncovering how age shapes the gut microbiota, as their microbial composition is highly similar to that of humans and is not easily affected by confounding factors	321:501	Non-human primates are an ideal model for uncovering how age shapes the gut microbiota, as their microbial composition is highly similar to that of humans and is not easily affected by confounding factors.
31837260	3	37	theme	Non-human	298:306	arg1	primates					308:315	Non-human primates	298:315	Non-human primates	298:315	Non-human primates are an ideal model for uncovering how age shapes the gut microbiota, as their microbial composition is highly similar to that of humans and is not easily affected by confounding factors.
31837260	5	38	theme	significant	708:718	arg1	differences					720:730	significant differences	708:730	significant differences in microbial composition among the three groups	708:778	Our findings revealed significant differences in microbial composition among the three groups.
31837260	6	39	theme	Coriobacteriaceae	845:861	arg1	abundances					814:823	the relative abundances	801:823	the relative abundances of Veillonellaceae, Coriobacteriaceae and Succinivibrionaceae were significantly increased, Ruminococcaceae and Rikenellaceae	801:949	With increased age, the relative abundances of Veillonellaceae, Coriobacteriaceae and Succinivibrionaceae were significantly increased, Ruminococcaceae and Rikenellaceae were significantly decreased at the family level.
31837260	7	40	theme	polysaccharides	1157:1171	arg1	metabolism					1173:1182	microbial polysaccharides metabolism	1147:1182	microbial polysaccharides metabolism	1147:1182	Functional enrichment showed that genes that differed among the three groups were mainly involved in arginine biosynthesis, purine metabolism and microbial polysaccharides metabolism.
31837260	5	41	from	differences	720:730	arg1	composition					745:755	microbial composition	735:755	microbial composition	735:755	Our findings revealed significant differences in microbial composition among the three groups.
31837260	6	42	theme	Ruminococcaceae	917:931	arg1	abundances					814:823	the relative abundances	801:823	the relative abundances of Veillonellaceae, Coriobacteriaceae and Succinivibrionaceae were significantly increased, Ruminococcaceae and Rikenellaceae	801:949	With increased age, the relative abundances of Veillonellaceae, Coriobacteriaceae and Succinivibrionaceae were significantly increased, Ruminococcaceae and Rikenellaceae were significantly decreased at the family level.
31837260	1	43	theme	disease	132:138	arg1	risk					140:143	disease risk	132:143	disease risk	132:143	Age can significantly affect human physiology and disease risk.
31837260	3	44	theme	ideal	324:328	arg1	model					330:334	an ideal model	321:334	an ideal model for uncovering how age shapes the gut microbiota, as their microbial composition is highly similar to that of humans and is not easily affected by confounding factors	321:501	Non-human primates are an ideal model for uncovering how age shapes the gut microbiota, as their microbial composition is highly similar to that of humans and is not easily affected by confounding factors.
31837260	3	44	theme	ideal	324:328	arg1	primates					308:315	Non-human primates	298:315	Non-human primates	298:315	Non-human primates are an ideal model for uncovering how age shapes the gut microbiota, as their microbial composition is highly similar to that of humans and is not easily affected by confounding factors.
31837260	6	45	theme	increased	786:794	arg1	age					796:798	increased age	786:798	increased age	786:798	With increased age, the relative abundances of Veillonellaceae, Coriobacteriaceae and Succinivibrionaceae were significantly increased, Ruminococcaceae and Rikenellaceae were significantly decreased at the family level.
31837260	7	46	theme	Functional	1001:1010	arg1	enrichment					1012:1021	Functional enrichment	1001:1021	Functional enrichment	1001:1021	Functional enrichment showed that genes that differed among the three groups were mainly involved in arginine biosynthesis, purine metabolism and microbial polysaccharides metabolism.
31837260	3	47	theme	confounding	483:493	arg1	factors					495:501	confounding factors	483:501	confounding factors	483:501	Non-human primates are an ideal model for uncovering how age shapes the gut microbiota, as their microbial composition is highly similar to that of humans and is not easily affected by confounding factors.
31837260	4	48	theme	microbial	586:594	arg1	phenotypes					596:605	the microbial phenotypes	582:605	the microbial phenotypes of 16 female cynomolgus macaques	582:638	Here, using the 16S rRNA and metagenomic sequencing methods, we characterized the microbial phenotypes of 16 female cynomolgus macaques from three age groups (young, adult and old).
31837260	0	49	theme	cynomolgus	61:70	arg1	macaques					72:79	cynomolgus macaques	61:79	cynomolgus macaques	61:79	Age-related changes in microbial composition and function in cynomolgus macaques.
31837260	2	50	theme	microbiota	232:241	arg1	function					212:219	function	212:219	function	212:219	Recent studies have shown that age may affect the composition and function of the gut microbiota, but the underlying mechanisms remain largely unknown.
31837260	2	50	theme	microbiota	232:241	arg1	composition					196:206	composition	196:206	composition	196:206	Recent studies have shown that age may affect the composition and function of the gut microbiota, but the underlying mechanisms remain largely unknown.
31837260	9	51	from	effects	1466:1472	arg1	body					1494:1497	the human body	1484:1497	the human body	1484:1497	In conclusion, we characterized the composition and function of the gut microbiota at different ages, and our findings provide a new entry point for understanding the effects of age on the human body.
31837260	4	52	theme	age	651:653	arg1	groups					655:660	three age groups	645:660	three age groups (young, adult and old)	645:683	Here, using the 16S rRNA and metagenomic sequencing methods, we characterized the microbial phenotypes of 16 female cynomolgus macaques from three age groups (young, adult and old).
31837260	2	53	dep	composition	196:206	arg1	the					192:194	the	192:194	the	192:194	Recent studies have shown that age may affect the composition and function of the gut microbiota, but the underlying mechanisms remain largely unknown.
31719838	1	0	theme	BACKGROUND	142:151	arg1	wolfberry					170:178	wolfberry	170:178	wolfberry	170:178	BACKGROUND Lycium barbarum (wolfberry) has been widely cultivated in China, particularly in northwest regions.
31719838	1	0	theme	BACKGROUND	142:151	arg1	barbarum					160:167	BACKGROUND Lycium barbarum	142:167	BACKGROUND Lycium barbarum (wolfberry)	142:179	BACKGROUND Lycium barbarum (wolfberry) has been widely cultivated in China, particularly in northwest regions.
31719838	4	1	theme	major	556:560	arg1	ingredients					569:579	major active ingredients	556:579	major active ingredients in L. barbarum which is crucial for its quality evaluation	556:638	Polysaccharides are considered major active ingredients in L. barbarum which is crucial for its quality evaluation.
31719838	0	2	from	evaluation	8:17	arg1	regions					65:71	different regions	55:71	different regions	55:71	Quality evaluation of Lycium barbarum (wolfberry) from different regions in China based on polysaccharide structure, yield and bioactivities.
31719838	0	2	from	evaluation	8:17	arg1	China					76:80	China	76:80	China	76:80	Quality evaluation of Lycium barbarum (wolfberry) from different regions in China based on polysaccharide structure, yield and bioactivities.
31719838	8	3	from	regions	1367:1373	arg1	LBPs					1347:1350	nine LBPs	1342:1350	nine LBPs from different regions	1342:1373	In addition, results showed that there was no significant difference in antioxidant and immune activities of nine LBPs from different regions.
31719838	8	3	from	regions	1367:1373	arg1	activities					1328:1337	antioxidant and immune activities	1305:1337	antioxidant and immune activities of nine LBPs from different regions	1305:1373	In addition, results showed that there was no significant difference in antioxidant and immune activities of nine LBPs from different regions.
31719838	1	4	theme	Lycium	153:158	arg1	wolfberry					170:178	wolfberry	170:178	wolfberry	170:178	BACKGROUND Lycium barbarum (wolfberry) has been widely cultivated in China, particularly in northwest regions.
31719838	1	4	theme	Lycium	153:158	arg1	barbarum					160:167	BACKGROUND Lycium barbarum	142:167	BACKGROUND Lycium barbarum (wolfberry)	142:179	BACKGROUND Lycium barbarum (wolfberry) has been widely cultivated in China, particularly in northwest regions.
31719838	1	5	theme	northwest	234:242	arg1	regions					244:250	northwest regions	234:250	northwest regions	234:250	BACKGROUND Lycium barbarum (wolfberry) has been widely cultivated in China, particularly in northwest regions.
31719838	4	6	from	ingredients	569:579	arg1	barbarum					587:594	L. barbarum	584:594	L. barbarum	584:594	Polysaccharides are considered major active ingredients in L. barbarum which is crucial for its quality evaluation.
31719838	7	7	theme	fractions	1054:1062	arg1	Mw					1040:1041	the Mw	1036:1041	the Mw of the two fractions (peaks 1-2)	1036:1074	RESULTS Our results showed that the similarity values of monosaccharide composition were larger than 0.926, and the Mw of the two fractions (peaks 1-2) in LBPs were ranging from 1.36 × 106 to 2.01 × 106 (peak 1), and 6.85 × 104 to 10.30 × 104 (peak 2) which indicated that the structure of LBPs were similar.
31719838	7	7	theme	fractions	1054:1062	arg1	0.926					1025:1029	0.926	1025:1029	0.926	1025:1029	RESULTS Our results showed that the similarity values of monosaccharide composition were larger than 0.926, and the Mw of the two fractions (peaks 1-2) in LBPs were ranging from 1.36 × 106 to 2.01 × 106 (peak 1), and 6.85 × 104 to 10.30 × 104 (peak 2) which indicated that the structure of LBPs were similar.
31719838	9	8	from	Province	1416:1423	arg1	yield					1389:1393	the yield	1385:1393	the yield of LBPs from Qinghai Province (low atmospheric temperature, high altitude)	1385:1468	However, the yield of LBPs from Qinghai Province (low atmospheric temperature, high altitude) was significantly lower (p < 0.05) than those collected from Xinjiang and Ningxia province.
31719838	9	8	from	Province	1416:1423	arg1	lower					1488:1492	lower	1488:1492	lower	1488:1492	However, the yield of LBPs from Qinghai Province (low atmospheric temperature, high altitude) was significantly lower (p < 0.05) than those collected from Xinjiang and Ningxia province.
31719838	9	8	from	Province	1416:1423	arg1	LBPs					1398:1401	LBPs	1398:1401	LBPs from Qinghai Province (low atmospheric temperature, high altitude)	1398:1468	However, the yield of LBPs from Qinghai Province (low atmospheric temperature, high altitude) was significantly lower (p < 0.05) than those collected from Xinjiang and Ningxia province.
31719838	2	9	theme	fruit	266:270	arg1	size					272:275	fruit size	266:275	fruit size	266:275	However, the fruit size and taste of L. barbarum from different habitats are quite different.
31719838	2	9	theme	fruit	266:270	arg1	different					336:344	different	336:344	different	336:344	However, the fruit size and taste of L. barbarum from different habitats are quite different.
31719838	11	10	theme	LBPs	1801:1804	arg1	control					1781:1787	the quality control	1769:1787	the quality control standard of LBPs	1769:1804	This study also provides a reference for improving the quality control standard of LBPs.
31719838	5	11	theme	monosaccharide	687:700	arg1	composition					702:712	monosaccharide composition	687:712	monosaccharide composition	687:712	METHODS In this study, we assessed the yield, monosaccharide composition, molecular weight, and conformation of L. barbarum polysaccharides (LBPs) collected from different regions of China.
31719838	9	12	theme	low	1426:1428	arg1	altitude					1460:1467	low atmospheric temperature, high altitude	1426:1467	altitude	1460:1467	However, the yield of LBPs from Qinghai Province (low atmospheric temperature, high altitude) was significantly lower (p < 0.05) than those collected from Xinjiang and Ningxia province.
31719838	9	12	theme	low	1426:1428	arg1	Province					1416:1423	Province	1416:1423	Province	1416:1423	However, the yield of LBPs from Qinghai Province (low atmospheric temperature, high altitude) was significantly lower (p < 0.05) than those collected from Xinjiang and Ningxia province.
31719838	5	13	theme	molecular	715:723	arg1	weight					725:730	molecular weight	715:730	molecular weight	715:730	METHODS In this study, we assessed the yield, monosaccharide composition, molecular weight, and conformation of L. barbarum polysaccharides (LBPs) collected from different regions of China.
31719838	4	14	theme	active	562:567	arg1	ingredients					569:579	major active ingredients	556:579	major active ingredients in L. barbarum which is crucial for its quality evaluation	556:638	Polysaccharides are considered major active ingredients in L. barbarum which is crucial for its quality evaluation.
31719838	0	15	theme	polysaccharide	91:104	arg1	structure					106:114	polysaccharide structure	91:114	polysaccharide structure	91:114	Quality evaluation of Lycium barbarum (wolfberry) from different regions in China based on polysaccharide structure, yield and bioactivities.
31719838	6	16	theme	immune	851:856	arg1	activities					858:867	The antioxidant and immune activities	831:867	The antioxidant and immune activities of LBPs	831:875	The antioxidant and immune activities of LBPs were also determined as its quality indicator.
31719838	6	16	theme	immune	851:856	arg1	indicator					913:921	its quality indicator	901:921	its quality indicator	901:921	The antioxidant and immune activities of LBPs were also determined as its quality indicator.
31719838	3	17	theme	responsible	487:497	arg1	mechanism					477:485	the detailed mechanism	464:485	the detailed mechanism responsible for this	464:506	Traditionally, only the fruit of L. barbarum produced in Ningxia province is recorded as an authentic herb, although the detailed mechanism responsible for this remains obscure.
31719838	7	18	theme	LBPs	1214:1217	arg1	structure					1201:1209	the structure	1197:1209	the structure of LBPs	1197:1217	RESULTS Our results showed that the similarity values of monosaccharide composition were larger than 0.926, and the Mw of the two fractions (peaks 1-2) in LBPs were ranging from 1.36 × 106 to 2.01 × 106 (peak 1), and 6.85 × 104 to 10.30 × 104 (peak 2) which indicated that the structure of LBPs were similar.
31719838	7	18	theme	LBPs	1214:1217	arg1	similar					1224:1230	similar	1224:1230	similar	1224:1230	RESULTS Our results showed that the similarity values of monosaccharide composition were larger than 0.926, and the Mw of the two fractions (peaks 1-2) in LBPs were ranging from 1.36 × 106 to 2.01 × 106 (peak 1), and 6.85 × 104 to 10.30 × 104 (peak 2) which indicated that the structure of LBPs were similar.
31719838	9	19	dep	lower	1488:1492	arg1	p < 0.05					1495:1502	p < 0.05	1495:1502	p < 0.05	1495:1502	However, the yield of LBPs from Qinghai Province (low atmospheric temperature, high altitude) was significantly lower (p < 0.05) than those collected from Xinjiang and Ningxia province.
31719838	9	20	from	yield	1389:1393	arg1	altitude					1460:1467	low atmospheric temperature, high altitude	1426:1467	altitude	1460:1467	However, the yield of LBPs from Qinghai Province (low atmospheric temperature, high altitude) was significantly lower (p < 0.05) than those collected from Xinjiang and Ningxia province.
31719838	9	20	from	yield	1389:1393	arg1	Province					1416:1423	Province	1416:1423	Province	1416:1423	However, the yield of LBPs from Qinghai Province (low atmospheric temperature, high altitude) was significantly lower (p < 0.05) than those collected from Xinjiang and Ningxia province.
31719838	5	21	dep	METHODS	641:647	arg1	assessed					667:674	assessed	667:674	assessed the yield, monosaccharide composition, molecular weight, and conformation of L. barbarum polysaccharides (LBPs) collected from different regions of China	667:828	METHODS In this study, we assessed the yield, monosaccharide composition, molecular weight, and conformation of L. barbarum polysaccharides (LBPs) collected from different regions of China.
31719838	4	22	theme	quality	621:627	arg1	evaluation					629:638	its quality evaluation	617:638	its quality evaluation	617:638	Polysaccharides are considered major active ingredients in L. barbarum which is crucial for its quality evaluation.
31719838	5	23	theme	polysaccharides	765:779	arg1	composition					702:712	monosaccharide composition	687:712	monosaccharide composition	687:712	METHODS In this study, we assessed the yield, monosaccharide composition, molecular weight, and conformation of L. barbarum polysaccharides (LBPs) collected from different regions of China.
31719838	5	23	theme	polysaccharides	765:779	arg1	weight					725:730	molecular weight	715:730	molecular weight	715:730	METHODS In this study, we assessed the yield, monosaccharide composition, molecular weight, and conformation of L. barbarum polysaccharides (LBPs) collected from different regions of China.
31719838	5	23	theme	polysaccharides	765:779	arg1	conformation					737:748	conformation	737:748	conformation	737:748	METHODS In this study, we assessed the yield, monosaccharide composition, molecular weight, and conformation of L. barbarum polysaccharides (LBPs) collected from different regions of China.
31719838	5	23	theme	polysaccharides	765:779	arg1	yield					680:684	the yield	676:684	the yield	676:684	METHODS In this study, we assessed the yield, monosaccharide composition, molecular weight, and conformation of L. barbarum polysaccharides (LBPs) collected from different regions of China.
31719838	6	24	theme	antioxidant	835:845	arg1	activities					858:867	The antioxidant and immune activities	831:867	The antioxidant and immune activities of LBPs	831:875	The antioxidant and immune activities of LBPs were also determined as its quality indicator.
31719838	6	24	theme	antioxidant	835:845	arg1	indicator					913:921	its quality indicator	901:921	its quality indicator	901:921	The antioxidant and immune activities of LBPs were also determined as its quality indicator.
31719838	2	25	from	habitats	317:324	arg1	taste					281:285	taste	281:285	taste	281:285	However, the fruit size and taste of L. barbarum from different habitats are quite different.
31719838	2	25	from	habitats	317:324	arg1	size					272:275	fruit size	266:275	fruit size	266:275	However, the fruit size and taste of L. barbarum from different habitats are quite different.
31719838	2	25	from	habitats	317:324	arg1	different					336:344	different	336:344	different	336:344	However, the fruit size and taste of L. barbarum from different habitats are quite different.
31719838	3	26	theme	barbarum	383:390	arg1	herb					449:452	an authentic herb	436:452	an authentic herb	436:452	Traditionally, only the fruit of L. barbarum produced in Ningxia province is recorded as an authentic herb, although the detailed mechanism responsible for this remains obscure.
31719838	3	26	theme	barbarum	383:390	arg1	fruit					371:375	only the fruit	362:375	only the fruit of L. barbarum produced in Ningxia province	362:419	Traditionally, only the fruit of L. barbarum produced in Ningxia province is recorded as an authentic herb, although the detailed mechanism responsible for this remains obscure.
31719838	0	27	theme	Quality	0:6	arg1	evaluation					8:17	Quality evaluation	0:17	Quality evaluation of Lycium barbarum (wolfberry) from different regions in China	0:80	Quality evaluation of Lycium barbarum (wolfberry) from different regions in China based on polysaccharide structure, yield and bioactivities.
31719838	5	28	theme	China	824:828	arg1	regions					813:819	different regions	803:819	different regions of China	803:828	METHODS In this study, we assessed the yield, monosaccharide composition, molecular weight, and conformation of L. barbarum polysaccharides (LBPs) collected from different regions of China.
31719838	8	29	from	activities	1328:1337	arg1	regions					1367:1373	different regions	1357:1373	different regions	1357:1373	In addition, results showed that there was no significant difference in antioxidant and immune activities of nine LBPs from different regions.
31719838	7	30	dep	10.30 × 104	1155:1165	arg1	to					1152:1153	to	1152:1153	to	1152:1153	RESULTS Our results showed that the similarity values of monosaccharide composition were larger than 0.926, and the Mw of the two fractions (peaks 1-2) in LBPs were ranging from 1.36 × 106 to 2.01 × 106 (peak 1), and 6.85 × 104 to 10.30 × 104 (peak 2) which indicated that the structure of LBPs were similar.
31719838	9	31	theme	atmospheric	1430:1440	arg1	altitude					1460:1467	low atmospheric temperature, high altitude	1426:1467	altitude	1460:1467	However, the yield of LBPs from Qinghai Province (low atmospheric temperature, high altitude) was significantly lower (p < 0.05) than those collected from Xinjiang and Ningxia province.
31719838	9	31	theme	atmospheric	1430:1440	arg1	Province					1416:1423	Province	1416:1423	Province	1416:1423	However, the yield of LBPs from Qinghai Province (low atmospheric temperature, high altitude) was significantly lower (p < 0.05) than those collected from Xinjiang and Ningxia province.
31719838	9	32	theme	LBPs	1398:1401	arg1	yield					1389:1393	the yield	1385:1393	the yield of LBPs from Qinghai Province (low atmospheric temperature, high altitude)	1385:1468	However, the yield of LBPs from Qinghai Province (low atmospheric temperature, high altitude) was significantly lower (p < 0.05) than those collected from Xinjiang and Ningxia province.
31719838	9	32	theme	LBPs	1398:1401	arg1	lower					1488:1492	lower	1488:1492	lower	1488:1492	However, the yield of LBPs from Qinghai Province (low atmospheric temperature, high altitude) was significantly lower (p < 0.05) than those collected from Xinjiang and Ningxia province.
31719838	0	33	from	regions	65:71	arg1	evaluation					8:17	Quality evaluation	0:17	Quality evaluation of Lycium barbarum (wolfberry) from different regions in China	0:80	Quality evaluation of Lycium barbarum (wolfberry) from different regions in China based on polysaccharide structure, yield and bioactivities.
31719838	8	34	theme	significant	1279:1289	arg1	difference					1291:1300	no significant difference	1276:1300	no significant difference	1276:1300	In addition, results showed that there was no significant difference in antioxidant and immune activities of nine LBPs from different regions.
31719838	5	35	theme	different	803:811	arg1	regions					813:819	different regions	803:819	different regions of China	803:828	METHODS In this study, we assessed the yield, monosaccharide composition, molecular weight, and conformation of L. barbarum polysaccharides (LBPs) collected from different regions of China.
31719838	0	36	theme	barbarum	29:36	arg1	evaluation					8:17	Quality evaluation	0:17	Quality evaluation of Lycium barbarum (wolfberry) from different regions in China	0:80	Quality evaluation of Lycium barbarum (wolfberry) from different regions in China based on polysaccharide structure, yield and bioactivities.
31719838	6	37	theme	quality	905:911	arg1	activities					858:867	The antioxidant and immune activities	831:867	The antioxidant and immune activities of LBPs	831:875	The antioxidant and immune activities of LBPs were also determined as its quality indicator.
31719838	6	37	theme	quality	905:911	arg1	indicator					913:921	its quality indicator	901:921	its quality indicator	901:921	The antioxidant and immune activities of LBPs were also determined as its quality indicator.
31719838	10	38	dep	Ningxia	1628:1634	arg1	maybe					1649:1653	maybe	1649:1653	maybe	1649:1653	CONCLUSIONS These data suggested that the L. barbarum produced in Ningxia and Xinjiang maybe more suitable as materials for medicines and functional foods.
31719838	5	39	theme	L.	753:754	arg1	LBPs					782:785	LBPs	782:785	LBPs	782:785	METHODS In this study, we assessed the yield, monosaccharide composition, molecular weight, and conformation of L. barbarum polysaccharides (LBPs) collected from different regions of China.
31719838	5	39	theme	L.	753:754	arg1	polysaccharides					765:779	L. barbarum polysaccharides	753:779	L. barbarum polysaccharides (LBPs) collected from different regions of China	753:828	METHODS In this study, we assessed the yield, monosaccharide composition, molecular weight, and conformation of L. barbarum polysaccharides (LBPs) collected from different regions of China.
31719838	7	40	dep	2.01 × 106	1116:1125	arg1	to					1113:1114	to	1113:1114	to	1113:1114	RESULTS Our results showed that the similarity values of monosaccharide composition were larger than 0.926, and the Mw of the two fractions (peaks 1-2) in LBPs were ranging from 1.36 × 106 to 2.01 × 106 (peak 1), and 6.85 × 104 to 10.30 × 104 (peak 2) which indicated that the structure of LBPs were similar.
31719838	8	41	theme	LBPs	1347:1350	arg1	activities					1328:1337	antioxidant and immune activities	1305:1337	antioxidant and immune activities of nine LBPs from different regions	1305:1373	In addition, results showed that there was no significant difference in antioxidant and immune activities of nine LBPs from different regions.
31719838	10	42	dep	CONCLUSIONS	1562:1572	arg1	suggested					1585:1593	suggested	1585:1593	suggested that the L. barbarum produced in Ningxia and Xinjiang maybe more suitable as materials for medicines and functional foods	1585:1715	CONCLUSIONS These data suggested that the L. barbarum produced in Ningxia and Xinjiang maybe more suitable as materials for medicines and functional foods.
31719838	7	43	theme	monosaccharide	981:994	arg1	composition					996:1006	monosaccharide composition	981:1006	monosaccharide composition	981:1006	RESULTS Our results showed that the similarity values of monosaccharide composition were larger than 0.926, and the Mw of the two fractions (peaks 1-2) in LBPs were ranging from 1.36 × 106 to 2.01 × 106 (peak 1), and 6.85 × 104 to 10.30 × 104 (peak 2) which indicated that the structure of LBPs were similar.
31719838	2	44	theme	different	307:315	arg1	habitats					317:324	different habitats	307:324	different habitats	307:324	However, the fruit size and taste of L. barbarum from different habitats are quite different.
31719838	5	45	theme	barbarum	756:763	arg1	LBPs					782:785	LBPs	782:785	LBPs	782:785	METHODS In this study, we assessed the yield, monosaccharide composition, molecular weight, and conformation of L. barbarum polysaccharides (LBPs) collected from different regions of China.
31719838	5	45	theme	barbarum	756:763	arg1	polysaccharides					765:779	L. barbarum polysaccharides	753:779	L. barbarum polysaccharides (LBPs) collected from different regions of China	753:828	METHODS In this study, we assessed the yield, monosaccharide composition, molecular weight, and conformation of L. barbarum polysaccharides (LBPs) collected from different regions of China.
31719838	6	46	theme	LBPs	872:875	arg1	activities					858:867	The antioxidant and immune activities	831:867	The antioxidant and immune activities of LBPs	831:875	The antioxidant and immune activities of LBPs were also determined as its quality indicator.
31719838	6	46	theme	LBPs	872:875	arg1	indicator					913:921	its quality indicator	901:921	its quality indicator	901:921	The antioxidant and immune activities of LBPs were also determined as its quality indicator.
31719838	11	47	theme	quality	1773:1779	arg1	control					1781:1787	the quality control	1769:1787	the quality control standard of LBPs	1769:1804	This study also provides a reference for improving the quality control standard of LBPs.
31719838	7	48	theme	similarity	960:969	arg1	larger					1013:1018	larger	1013:1018	larger	1013:1018	RESULTS Our results showed that the similarity values of monosaccharide composition were larger than 0.926, and the Mw of the two fractions (peaks 1-2) in LBPs were ranging from 1.36 × 106 to 2.01 × 106 (peak 1), and 6.85 × 104 to 10.30 × 104 (peak 2) which indicated that the structure of LBPs were similar.
31719838	7	48	theme	similarity	960:969	arg1	values					971:976	the similarity values	956:976	the similarity values of monosaccharide composition	956:1006	RESULTS Our results showed that the similarity values of monosaccharide composition were larger than 0.926, and the Mw of the two fractions (peaks 1-2) in LBPs were ranging from 1.36 × 106 to 2.01 × 106 (peak 1), and 6.85 × 104 to 10.30 × 104 (peak 2) which indicated that the structure of LBPs were similar.
31719838	9	49	theme	high	1455:1458	arg1	altitude					1460:1467	low atmospheric temperature, high altitude	1426:1467	altitude	1460:1467	However, the yield of LBPs from Qinghai Province (low atmospheric temperature, high altitude) was significantly lower (p < 0.05) than those collected from Xinjiang and Ningxia province.
31719838	9	49	theme	high	1455:1458	arg1	Province					1416:1423	Province	1416:1423	Province	1416:1423	However, the yield of LBPs from Qinghai Province (low atmospheric temperature, high altitude) was significantly lower (p < 0.05) than those collected from Xinjiang and Ningxia province.
31719838	10	50	theme	functional	1700:1709	arg1	foods					1711:1715	functional foods	1700:1715	functional foods	1700:1715	CONCLUSIONS These data suggested that the L. barbarum produced in Ningxia and Xinjiang maybe more suitable as materials for medicines and functional foods.
31719838	7	51	theme	composition	996:1006	arg1	larger					1013:1018	larger	1013:1018	larger	1013:1018	RESULTS Our results showed that the similarity values of monosaccharide composition were larger than 0.926, and the Mw of the two fractions (peaks 1-2) in LBPs were ranging from 1.36 × 106 to 2.01 × 106 (peak 1), and 6.85 × 104 to 10.30 × 104 (peak 2) which indicated that the structure of LBPs were similar.
31719838	7	51	theme	composition	996:1006	arg1	values					971:976	the similarity values	956:976	the similarity values of monosaccharide composition	956:1006	RESULTS Our results showed that the similarity values of monosaccharide composition were larger than 0.926, and the Mw of the two fractions (peaks 1-2) in LBPs were ranging from 1.36 × 106 to 2.01 × 106 (peak 1), and 6.85 × 104 to 10.30 × 104 (peak 2) which indicated that the structure of LBPs were similar.
31719838	8	52	theme	different	1357:1365	arg1	regions					1367:1373	different regions	1357:1373	different regions	1357:1373	In addition, results showed that there was no significant difference in antioxidant and immune activities of nine LBPs from different regions.
31719838	2	53	theme	barbarum	293:300	arg1	taste					281:285	taste	281:285	taste	281:285	However, the fruit size and taste of L. barbarum from different habitats are quite different.
31719838	2	53	theme	barbarum	293:300	arg1	size					272:275	fruit size	266:275	fruit size	266:275	However, the fruit size and taste of L. barbarum from different habitats are quite different.
31719838	2	53	theme	barbarum	293:300	arg1	different					336:344	different	336:344	different	336:344	However, the fruit size and taste of L. barbarum from different habitats are quite different.
31719838	10	54	theme	L.	1604:1605	arg1	barbarum					1607:1614	the L. barbarum	1600:1614	the L. barbarum	1600:1614	CONCLUSIONS These data suggested that the L. barbarum produced in Ningxia and Xinjiang maybe more suitable as materials for medicines and functional foods.
31719838	9	55	theme	temperature	1442:1452	arg1	altitude					1460:1467	low atmospheric temperature, high altitude	1426:1467	altitude	1460:1467	However, the yield of LBPs from Qinghai Province (low atmospheric temperature, high altitude) was significantly lower (p < 0.05) than those collected from Xinjiang and Ningxia province.
31719838	9	55	theme	temperature	1442:1452	arg1	Province					1416:1423	Province	1416:1423	Province	1416:1423	However, the yield of LBPs from Qinghai Province (low atmospheric temperature, high altitude) was significantly lower (p < 0.05) than those collected from Xinjiang and Ningxia province.
31719838	8	56	theme	antioxidant	1305:1315	arg1	activities					1328:1337	antioxidant and immune activities	1305:1337	antioxidant and immune activities of nine LBPs from different regions	1305:1373	In addition, results showed that there was no significant difference in antioxidant and immune activities of nine LBPs from different regions.
31719838	3	57	theme	authentic	439:447	arg1	herb					449:452	an authentic herb	436:452	an authentic herb	436:452	Traditionally, only the fruit of L. barbarum produced in Ningxia province is recorded as an authentic herb, although the detailed mechanism responsible for this remains obscure.
31719838	3	57	theme	authentic	439:447	arg1	fruit					371:375	only the fruit	362:375	only the fruit of L. barbarum produced in Ningxia province	362:419	Traditionally, only the fruit of L. barbarum produced in Ningxia province is recorded as an authentic herb, although the detailed mechanism responsible for this remains obscure.
31719838	7	58	dep	RESULTS	924:930	arg1	showed					944:949	showed	944:949	showed that the similarity values of monosaccharide composition were larger than 0.926, and the Mw of the two fractions (peaks 1-2) in LBPs	944:1082	RESULTS Our results showed that the similarity values of monosaccharide composition were larger than 0.926, and the Mw of the two fractions (peaks 1-2) in LBPs were ranging from 1.36 × 106 to 2.01 × 106 (peak 1), and 6.85 × 104 to 10.30 × 104 (peak 2) which indicated that the structure of LBPs were similar.
31719838	0	59	theme	different	55:63	arg1	regions					65:71	different regions	55:71	different regions	55:71	Quality evaluation of Lycium barbarum (wolfberry) from different regions in China based on polysaccharide structure, yield and bioactivities.
31719838	3	60	theme	detailed	468:475	arg1	mechanism					477:485	the detailed mechanism	464:485	the detailed mechanism responsible for this	464:506	Traditionally, only the fruit of L. barbarum produced in Ningxia province is recorded as an authentic herb, although the detailed mechanism responsible for this remains obscure.
31719838	3	61	theme	Ningxia	404:410	arg1	province					412:419	Ningxia province	404:419	Ningxia province	404:419	Traditionally, only the fruit of L. barbarum produced in Ningxia province is recorded as an authentic herb, although the detailed mechanism responsible for this remains obscure.
31719838	8	62	theme	immune	1321:1326	arg1	activities					1328:1337	antioxidant and immune activities	1305:1337	antioxidant and immune activities of nine LBPs from different regions	1305:1373	In addition, results showed that there was no significant difference in antioxidant and immune activities of nine LBPs from different regions.
30338938	6	0	dep	structure	705:713	arg1	The					701:703	The	701:703	The	701:703	The structure and composition of humins are very diverse and depend on the thermochemical conditions.
30338938	4	1	with	humins	481:486	arg1	emphasis					501:508	special emphasis	493:508	special emphasis on their structure/properties relationships	493:552	This work focuses on the comprehensive understanding of humins with special emphasis on their structure/properties relationships.
30338938	4	2	theme	structure/properties	519:538	arg1	relationships					540:552	their structure/properties relationships	513:552	their structure/properties relationships	513:552	This work focuses on the comprehensive understanding of humins with special emphasis on their structure/properties relationships.
30338938	5	3	theme	structural	639:648	arg1	investigations					685:698	structural, thermoanalytical, and rheological investigations	639:698	structural, thermoanalytical, and rheological investigations	639:698	Humins were subjected to different thermal treatments and characterized by means of structural, thermoanalytical, and rheological investigations.
30338938	3	4	theme	new	366:368	arg1	solutions					370:378	new solutions	366:378	new solutions for humins valorization	366:402	To improve the efficiency of this process from an economical and environmental perspective, new solutions for humins valorization are urgently needed.
30338938	7	5	theme	high	915:918	arg1	temperature					937:947	a relatively high glass-transition temperature	902:947	a relatively high glass-transition temperature (Tg >65 °C)	902:959	On sufficient heating, humins change into a nonreversible and more branched furanic structure with a relatively high glass-transition temperature (Tg >65 °C).
30338938	7	5	theme	high	915:918	arg1	>65 °C					953:958	Tg >65 °C	950:958	Tg >65 °C	950:958	On sufficient heating, humins change into a nonreversible and more branched furanic structure with a relatively high glass-transition temperature (Tg >65 °C).
30338938	3	6	theme	environmental	339:351	arg1	perspective					353:363	an economical and environmental perspective	321:363	an economical and environmental perspective	321:363	To improve the efficiency of this process from an economical and environmental perspective, new solutions for humins valorization are urgently needed.
30338938	4	7	theme	humins	481:486	arg1	understanding					464:476	the comprehensive understanding	446:476	the comprehensive understanding of humins with special emphasis on their structure/properties relationships	446:552	This work focuses on the comprehensive understanding of humins with special emphasis on their structure/properties relationships.
30338938	4	8	from	emphasis	501:508	arg1	relationships					540:552	their structure/properties relationships	513:552	their structure/properties relationships	513:552	This work focuses on the comprehensive understanding of humins with special emphasis on their structure/properties relationships.
30338938	5	9	theme	thermoanalytical	651:666	arg1	investigations					685:698	structural, thermoanalytical, and rheological investigations	639:698	structural, thermoanalytical, and rheological investigations	639:698	Humins were subjected to different thermal treatments and characterized by means of structural, thermoanalytical, and rheological investigations.
30338938	1	10	theme	lignocellulosic	82:96	arg1	biomass					98:104	lignocellulosic biomass	82:104	lignocellulosic biomass	82:104	Conversion of lignocellulosic biomass often brings about the formation of several side products.
30338938	7	11	theme	glass-transition	920:935	arg1	temperature					937:947	a relatively high glass-transition temperature	902:947	a relatively high glass-transition temperature (Tg >65 °C)	902:959	On sufficient heating, humins change into a nonreversible and more branched furanic structure with a relatively high glass-transition temperature (Tg >65 °C).
30338938	7	11	theme	glass-transition	920:935	arg1	>65 °C					953:958	Tg >65 °C	950:958	Tg >65 °C	950:958	On sufficient heating, humins change into a nonreversible and more branched furanic structure with a relatively high glass-transition temperature (Tg >65 °C).
30338938	1	12	theme	biomass	98:104	arg1	Conversion					68:77	Conversion	68:77	Conversion of lignocellulosic biomass	68:104	Conversion of lignocellulosic biomass often brings about the formation of several side products.
30338938	3	13	theme	humins	384:389	arg1	valorization					391:402	humins valorization	384:402	humins valorization	384:402	To improve the efficiency of this process from an economical and environmental perspective, new solutions for humins valorization are urgently needed.
30338938	4	14	theme	special	493:499	arg1	emphasis					501:508	special emphasis	493:508	special emphasis on their structure/properties relationships	493:552	This work focuses on the comprehensive understanding of humins with special emphasis on their structure/properties relationships.
30338938	5	15	theme	rheological	673:683	arg1	investigations					685:698	structural, thermoanalytical, and rheological investigations	639:698	structural, thermoanalytical, and rheological investigations	639:698	Humins were subjected to different thermal treatments and characterized by means of structural, thermoanalytical, and rheological investigations.
30338938	3	16	from	perspective	353:363	arg1	efficiency					289:298	the efficiency	285:298	the efficiency of this process from an economical and environmental perspective	285:363	To improve the efficiency of this process from an economical and environmental perspective, new solutions for humins valorization are urgently needed.
30338938	6	17	theme	humins	734:739	arg1	composition					719:729	composition	719:729	composition	719:729	The structure and composition of humins are very diverse and depend on the thermochemical conditions.
30338938	6	17	theme	humins	734:739	arg1	structure					705:713	structure	705:713	structure	705:713	The structure and composition of humins are very diverse and depend on the thermochemical conditions.
30338938	4	18	theme	comprehensive	450:462	arg1	understanding					464:476	the comprehensive understanding	446:476	the comprehensive understanding of humins with special emphasis on their structure/properties relationships	446:552	This work focuses on the comprehensive understanding of humins with special emphasis on their structure/properties relationships.
30338938	7	19	theme	branched	870:877	arg1	structure					887:895	a nonreversible and more branched furanic structure	845:895	a nonreversible and more branched furanic structure	845:895	On sufficient heating, humins change into a nonreversible and more branched furanic structure with a relatively high glass-transition temperature (Tg >65 °C).
30338938	0	20	theme	Macromolecular	44:57	arg1	Systems					59:65	Thermoreactive Macromolecular Systems	29:65	Thermoreactive Macromolecular Systems	29:65	Humins from Biorefineries as Thermoreactive Macromolecular Systems.
30338938	7	21	theme	nonreversible	847:859	arg1	structure					887:895	a nonreversible and more branched furanic structure	845:895	a nonreversible and more branched furanic structure	845:895	On sufficient heating, humins change into a nonreversible and more branched furanic structure with a relatively high glass-transition temperature (Tg >65 °C).
30338938	2	22	theme	black	180:184	arg1	coproduct					198:206	a black and viscous coproduct	178:206	a black and viscous coproduct known as humins	178:222	Among these, a black and viscous coproduct known as humins is formed on acidic treatment of polysaccharides.
30338938	7	23	theme	furanic	879:885	arg1	structure					887:895	a nonreversible and more branched furanic structure	845:895	a nonreversible and more branched furanic structure	845:895	On sufficient heating, humins change into a nonreversible and more branched furanic structure with a relatively high glass-transition temperature (Tg >65 °C).
30338938	0	24	theme	Thermoreactive	29:42	arg1	Systems					59:65	Thermoreactive Macromolecular Systems	29:65	Thermoreactive Macromolecular Systems	29:65	Humins from Biorefineries as Thermoreactive Macromolecular Systems.
30338938	5	25	theme	different	580:588	arg1	treatments					598:607	different thermal treatments	580:607	different thermal treatments	580:607	Humins were subjected to different thermal treatments and characterized by means of structural, thermoanalytical, and rheological investigations.
30338938	3	26	theme	process	308:314	arg1	efficiency					289:298	the efficiency	285:298	the efficiency of this process from an economical and environmental perspective	285:363	To improve the efficiency of this process from an economical and environmental perspective, new solutions for humins valorization are urgently needed.
30338938	5	27	theme	thermal	590:596	arg1	treatments					598:607	different thermal treatments	580:607	different thermal treatments	580:607	Humins were subjected to different thermal treatments and characterized by means of structural, thermoanalytical, and rheological investigations.
30338938	2	28	theme	polysaccharides	257:271	arg1	treatment					244:252	acidic treatment	237:252	acidic treatment of polysaccharides	237:271	Among these, a black and viscous coproduct known as humins is formed on acidic treatment of polysaccharides.
30338938	1	29	theme	several	142:148	arg1	products					155:162	several side products	142:162	several side products	142:162	Conversion of lignocellulosic biomass often brings about the formation of several side products.
30338938	7	30	theme	sufficient	806:815	arg1	heating					817:823	sufficient heating	806:823	sufficient heating	806:823	On sufficient heating, humins change into a nonreversible and more branched furanic structure with a relatively high glass-transition temperature (Tg >65 °C).
30338938	1	31	theme	side	150:153	arg1	products					155:162	several side products	142:162	several side products	142:162	Conversion of lignocellulosic biomass often brings about the formation of several side products.
30338938	3	32	theme	economical	324:333	arg1	perspective					353:363	an economical and environmental perspective	321:363	an economical and environmental perspective	321:363	To improve the efficiency of this process from an economical and environmental perspective, new solutions for humins valorization are urgently needed.
30338938	2	33	theme	viscous	190:196	arg1	coproduct					198:206	a black and viscous coproduct	178:206	a black and viscous coproduct known as humins	178:222	Among these, a black and viscous coproduct known as humins is formed on acidic treatment of polysaccharides.
30338938	6	34	theme	thermochemical	776:789	arg1	conditions					791:800	the thermochemical conditions	772:800	the thermochemical conditions	772:800	The structure and composition of humins are very diverse and depend on the thermochemical conditions.
30338938	1	35	theme	products	155:162	arg1	formation					129:137	the formation	125:137	the formation of several side products	125:162	Conversion of lignocellulosic biomass often brings about the formation of several side products.
30338938	2	36	theme	acidic	237:242	arg1	treatment					244:252	acidic treatment	237:252	acidic treatment of polysaccharides	237:271	Among these, a black and viscous coproduct known as humins is formed on acidic treatment of polysaccharides.
30338938	8	37	theme	thermoset-like	1013:1026	arg1	resins					1028:1033	thermoset-like resins	1013:1033	thermoset-like resins	1013:1033	Thus, humins can be easily processed for preparing thermoset-like resins.
30338938	7	38	theme	Tg	950:951	arg1	temperature					937:947	a relatively high glass-transition temperature	902:947	a relatively high glass-transition temperature (Tg >65 °C)	902:959	On sufficient heating, humins change into a nonreversible and more branched furanic structure with a relatively high glass-transition temperature (Tg >65 °C).
30338938	7	38	theme	Tg	950:951	arg1	>65 °C					953:958	Tg >65 °C	950:958	Tg >65 °C	950:958	On sufficient heating, humins change into a nonreversible and more branched furanic structure with a relatively high glass-transition temperature (Tg >65 °C).
29528237	0	0	theme	s	63:63	arg1	Synthesis					25:33	the Synthesis	21:33	the Synthesis of C-N-Linked Bi(heteroaryl)s Using Heteroaryl Ethers and N-Benzoyl Heteroarenes	21:114	Catalytic Method for the Synthesis of C-N-Linked Bi(heteroaryl)s Using Heteroaryl Ethers and N-Benzoyl Heteroarenes.
29528237	1	1	theme	heteroaryl	131:140	arg1	s					142:142	C-N-linked bi(heteroaryl)s	117:142	C-N-linked bi(heteroaryl)s	117:142	C-N-linked bi(heteroaryl)s are synthesized by a rhodium-catalyzed N-heteroarylation reaction of N-benzoyl heteroarenes including azoles/azolones, pyridones, cyclic ureas, and cyclic imides using heteroaryl aryl ethers.
29528237	2	2	theme	five-	535:539	arg1	heteroarenes					558:569	five- and six-membered heteroarenes	535:569	five- and six-membered heteroarenes	535:569	The reaction involves the covalent bond-exchange reaction of N-CO and HetAr-O bonds without using metal bases and exhibits a broad applicability, giving diverse C-N-linked bi(heteroaryl)s containing five- and six-membered heteroarenes.
29528237	0	3	theme	Heteroaryl	71:80	arg1	Ethers					82:87	Heteroaryl Ethers	71:87	Heteroaryl Ethers	71:87	Catalytic Method for the Synthesis of C-N-Linked Bi(heteroaryl)s Using Heteroaryl Ethers and N-Benzoyl Heteroarenes.
29528237	2	4	theme	heteroaryl	511:520	arg1	s					522:522	diverse C-N-linked bi(heteroaryl)s	489:522	diverse C-N-linked bi(heteroaryl)s containing five- and six-membered heteroarenes	489:569	The reaction involves the covalent bond-exchange reaction of N-CO and HetAr-O bonds without using metal bases and exhibits a broad applicability, giving diverse C-N-linked bi(heteroaryl)s containing five- and six-membered heteroarenes.
29528237	2	5	theme	bonds	414:418	arg1	reaction					385:392	the covalent bond-exchange reaction	358:392	the covalent bond-exchange reaction of N-CO and HetAr-O bonds	358:418	The reaction involves the covalent bond-exchange reaction of N-CO and HetAr-O bonds without using metal bases and exhibits a broad applicability, giving diverse C-N-linked bi(heteroaryl)s containing five- and six-membered heteroarenes.
29528237	2	6	contain	containing	524:533	arg1	s					522:522	diverse C-N-linked bi(heteroaryl)s	489:522	diverse C-N-linked bi(heteroaryl)s containing five- and six-membered heteroarenes	489:569	The reaction involves the covalent bond-exchange reaction of N-CO and HetAr-O bonds without using metal bases and exhibits a broad applicability, giving diverse C-N-linked bi(heteroaryl)s containing five- and six-membered heteroarenes.
29528237	2	6	contain	containing	524:533	arg2	heteroarenes					558:569	five- and six-membered heteroarenes	535:569	five- and six-membered heteroarenes	535:569	The reaction involves the covalent bond-exchange reaction of N-CO and HetAr-O bonds without using metal bases and exhibits a broad applicability, giving diverse C-N-linked bi(heteroaryl)s containing five- and six-membered heteroarenes.
29528237	2	7	theme	N-CO	397:400	arg1	bonds					414:418	N-CO and HetAr-O bonds	397:418	N-CO and HetAr-O bonds	397:418	The reaction involves the covalent bond-exchange reaction of N-CO and HetAr-O bonds without using metal bases and exhibits a broad applicability, giving diverse C-N-linked bi(heteroaryl)s containing five- and six-membered heteroarenes.
29528237	2	8	theme	bi	508:509	arg1	s					522:522	diverse C-N-linked bi(heteroaryl)s	489:522	diverse C-N-linked bi(heteroaryl)s containing five- and six-membered heteroarenes	489:569	The reaction involves the covalent bond-exchange reaction of N-CO and HetAr-O bonds without using metal bases and exhibits a broad applicability, giving diverse C-N-linked bi(heteroaryl)s containing five- and six-membered heteroarenes.
29528237	2	9	theme	covalent	362:369	arg1	reaction					385:392	the covalent bond-exchange reaction	358:392	the covalent bond-exchange reaction of N-CO and HetAr-O bonds	358:418	The reaction involves the covalent bond-exchange reaction of N-CO and HetAr-O bonds without using metal bases and exhibits a broad applicability, giving diverse C-N-linked bi(heteroaryl)s containing five- and six-membered heteroarenes.
29528237	2	10	theme	C-N-linked	497:506	arg1	s					522:522	diverse C-N-linked bi(heteroaryl)s	489:522	diverse C-N-linked bi(heteroaryl)s containing five- and six-membered heteroarenes	489:569	The reaction involves the covalent bond-exchange reaction of N-CO and HetAr-O bonds without using metal bases and exhibits a broad applicability, giving diverse C-N-linked bi(heteroaryl)s containing five- and six-membered heteroarenes.
29528237	0	11	theme	Catalytic	0:8	arg1	Method					10:15	Catalytic Method	0:15	Catalytic Method for the Synthesis of C-N-Linked Bi(heteroaryl)s Using Heteroaryl Ethers and N-Benzoyl Heteroarenes.	0:115	Catalytic Method for the Synthesis of C-N-Linked Bi(heteroaryl)s Using Heteroaryl Ethers and N-Benzoyl Heteroarenes.
29528237	1	12	theme	cyclic	274:279	arg1	ureas					281:285	cyclic ureas	274:285	cyclic ureas	274:285	C-N-linked bi(heteroaryl)s are synthesized by a rhodium-catalyzed N-heteroarylation reaction of N-benzoyl heteroarenes including azoles/azolones, pyridones, cyclic ureas, and cyclic imides using heteroaryl aryl ethers.
29528237	2	13	theme	bond-exchange	371:383	arg1	reaction					385:392	the covalent bond-exchange reaction	358:392	the covalent bond-exchange reaction of N-CO and HetAr-O bonds	358:418	The reaction involves the covalent bond-exchange reaction of N-CO and HetAr-O bonds without using metal bases and exhibits a broad applicability, giving diverse C-N-linked bi(heteroaryl)s containing five- and six-membered heteroarenes.
29528237	2	14	theme	metal	434:438	arg1	bases					440:444	metal bases	434:444	metal bases	434:444	The reaction involves the covalent bond-exchange reaction of N-CO and HetAr-O bonds without using metal bases and exhibits a broad applicability, giving diverse C-N-linked bi(heteroaryl)s containing five- and six-membered heteroarenes.
29528237	0	15	theme	N-Benzoyl	93:101	arg1	Heteroarenes					103:114	N-Benzoyl Heteroarenes	93:114	N-Benzoyl Heteroarenes	93:114	Catalytic Method for the Synthesis of C-N-Linked Bi(heteroaryl)s Using Heteroaryl Ethers and N-Benzoyl Heteroarenes.
29528237	1	16	theme	rhodium-catalyzed	165:181	arg1	reaction					201:208	a rhodium-catalyzed N-heteroarylation reaction	163:208	a rhodium-catalyzed N-heteroarylation reaction of N-benzoyl heteroarenes including azoles/azolones, pyridones, cyclic ureas, and cyclic imides using heteroaryl aryl ethers	163:333	C-N-linked bi(heteroaryl)s are synthesized by a rhodium-catalyzed N-heteroarylation reaction of N-benzoyl heteroarenes including azoles/azolones, pyridones, cyclic ureas, and cyclic imides using heteroaryl aryl ethers.
29528237	2	17	theme	HetAr-O	406:412	arg1	bonds					414:418	N-CO and HetAr-O bonds	397:418	N-CO and HetAr-O bonds	397:418	The reaction involves the covalent bond-exchange reaction of N-CO and HetAr-O bonds without using metal bases and exhibits a broad applicability, giving diverse C-N-linked bi(heteroaryl)s containing five- and six-membered heteroarenes.
29528237	3	18	theme	N-H	597:599	arg1	azoles/azolones					601:615	N-H azoles/azolones	597:615	N-H azoles/azolones	597:615	The N-heteroarylation of N-H azoles/azolones and pyridone proceeds at higher reaction temperatures.
29528237	1	19	theme	N-heteroarylation	183:199	arg1	reaction					201:208	a rhodium-catalyzed N-heteroarylation reaction	163:208	a rhodium-catalyzed N-heteroarylation reaction of N-benzoyl heteroarenes including azoles/azolones, pyridones, cyclic ureas, and cyclic imides using heteroaryl aryl ethers	163:333	C-N-linked bi(heteroaryl)s are synthesized by a rhodium-catalyzed N-heteroarylation reaction of N-benzoyl heteroarenes including azoles/azolones, pyridones, cyclic ureas, and cyclic imides using heteroaryl aryl ethers.
29528237	2	20	theme	broad	461:465	arg1	applicability					467:479	a broad applicability	459:479	a broad applicability	459:479	The reaction involves the covalent bond-exchange reaction of N-CO and HetAr-O bonds without using metal bases and exhibits a broad applicability, giving diverse C-N-linked bi(heteroaryl)s containing five- and six-membered heteroarenes.
29528237	1	21	theme	cyclic	292:297	arg1	imides					299:304	cyclic imides	292:304	cyclic imides	292:304	C-N-linked bi(heteroaryl)s are synthesized by a rhodium-catalyzed N-heteroarylation reaction of N-benzoyl heteroarenes including azoles/azolones, pyridones, cyclic ureas, and cyclic imides using heteroaryl aryl ethers.
29528237	3	22	theme	higher	642:647	arg1	temperatures					658:669	higher reaction temperatures	642:669	higher reaction temperatures	642:669	The N-heteroarylation of N-H azoles/azolones and pyridone proceeds at higher reaction temperatures.
29528237	3	23	theme	azoles/azolones	601:615	arg1	N-heteroarylation					576:592	The N-heteroarylation	572:592	The N-heteroarylation of N-H azoles/azolones and pyridone	572:628	The N-heteroarylation of N-H azoles/azolones and pyridone proceeds at higher reaction temperatures.
29528237	0	24	theme	Bi	49:50	arg1	s					63:63	C-N-Linked Bi(heteroaryl)s	38:63	C-N-Linked Bi(heteroaryl)s Using Heteroaryl Ethers and N-Benzoyl Heteroarenes	38:114	Catalytic Method for the Synthesis of C-N-Linked Bi(heteroaryl)s Using Heteroaryl Ethers and N-Benzoyl Heteroarenes.
29528237	3	25	theme	reaction	649:656	arg1	temperatures					658:669	higher reaction temperatures	642:669	higher reaction temperatures	642:669	The N-heteroarylation of N-H azoles/azolones and pyridone proceeds at higher reaction temperatures.
29528237	0	26	theme	C-N-Linked	38:47	arg1	s					63:63	C-N-Linked Bi(heteroaryl)s	38:63	C-N-Linked Bi(heteroaryl)s Using Heteroaryl Ethers and N-Benzoyl Heteroarenes	38:114	Catalytic Method for the Synthesis of C-N-Linked Bi(heteroaryl)s Using Heteroaryl Ethers and N-Benzoyl Heteroarenes.
29528237	1	27	link	C-N-linked	117:126	arg1	s					142:142	C-N-linked bi(heteroaryl)s	117:142	C-N-linked bi(heteroaryl)s	117:142	C-N-linked bi(heteroaryl)s are synthesized by a rhodium-catalyzed N-heteroarylation reaction of N-benzoyl heteroarenes including azoles/azolones, pyridones, cyclic ureas, and cyclic imides using heteroaryl aryl ethers.
29528237	2	28	link	C-N-linked	497:506	arg1	s					522:522	diverse C-N-linked bi(heteroaryl)s	489:522	diverse C-N-linked bi(heteroaryl)s containing five- and six-membered heteroarenes	489:569	The reaction involves the covalent bond-exchange reaction of N-CO and HetAr-O bonds without using metal bases and exhibits a broad applicability, giving diverse C-N-linked bi(heteroaryl)s containing five- and six-membered heteroarenes.
29528237	1	29	theme	heteroaryl	312:321	arg1	ethers					328:333	heteroaryl aryl ethers	312:333	heteroaryl aryl ethers	312:333	C-N-linked bi(heteroaryl)s are synthesized by a rhodium-catalyzed N-heteroarylation reaction of N-benzoyl heteroarenes including azoles/azolones, pyridones, cyclic ureas, and cyclic imides using heteroaryl aryl ethers.
29528237	2	30	theme	six-membered	545:556	arg1	heteroarenes					558:569	five- and six-membered heteroarenes	535:569	five- and six-membered heteroarenes	535:569	The reaction involves the covalent bond-exchange reaction of N-CO and HetAr-O bonds without using metal bases and exhibits a broad applicability, giving diverse C-N-linked bi(heteroaryl)s containing five- and six-membered heteroarenes.
29528237	1	31	theme	C-N-linked	117:126	arg1	s					142:142	C-N-linked bi(heteroaryl)s	117:142	C-N-linked bi(heteroaryl)s	117:142	C-N-linked bi(heteroaryl)s are synthesized by a rhodium-catalyzed N-heteroarylation reaction of N-benzoyl heteroarenes including azoles/azolones, pyridones, cyclic ureas, and cyclic imides using heteroaryl aryl ethers.
29528237	1	32	theme	N-benzoyl	213:221	arg1	heteroarenes					223:234	N-benzoyl heteroarenes	213:234	N-benzoyl heteroarenes including azoles/azolones, pyridones, cyclic ureas, and cyclic imides using heteroaryl aryl ethers	213:333	C-N-linked bi(heteroaryl)s are synthesized by a rhodium-catalyzed N-heteroarylation reaction of N-benzoyl heteroarenes including azoles/azolones, pyridones, cyclic ureas, and cyclic imides using heteroaryl aryl ethers.
29528237	1	32	theme	N-benzoyl	213:221	arg1	imides					299:304	cyclic imides	292:304	cyclic imides	292:304	C-N-linked bi(heteroaryl)s are synthesized by a rhodium-catalyzed N-heteroarylation reaction of N-benzoyl heteroarenes including azoles/azolones, pyridones, cyclic ureas, and cyclic imides using heteroaryl aryl ethers.
29528237	1	32	theme	N-benzoyl	213:221	arg1	pyridones					263:271	pyridones	263:271	pyridones	263:271	C-N-linked bi(heteroaryl)s are synthesized by a rhodium-catalyzed N-heteroarylation reaction of N-benzoyl heteroarenes including azoles/azolones, pyridones, cyclic ureas, and cyclic imides using heteroaryl aryl ethers.
29528237	1	32	theme	N-benzoyl	213:221	arg1	ureas					281:285	cyclic ureas	274:285	cyclic ureas	274:285	C-N-linked bi(heteroaryl)s are synthesized by a rhodium-catalyzed N-heteroarylation reaction of N-benzoyl heteroarenes including azoles/azolones, pyridones, cyclic ureas, and cyclic imides using heteroaryl aryl ethers.
29528237	1	32	theme	N-benzoyl	213:221	arg1	azoles/azolones					246:260	azoles/azolones	246:260	azoles/azolones	246:260	C-N-linked bi(heteroaryl)s are synthesized by a rhodium-catalyzed N-heteroarylation reaction of N-benzoyl heteroarenes including azoles/azolones, pyridones, cyclic ureas, and cyclic imides using heteroaryl aryl ethers.
29528237	0	33	theme	heteroaryl	52:61	arg1	s					63:63	C-N-Linked Bi(heteroaryl)s	38:63	C-N-Linked Bi(heteroaryl)s Using Heteroaryl Ethers and N-Benzoyl Heteroarenes	38:114	Catalytic Method for the Synthesis of C-N-Linked Bi(heteroaryl)s Using Heteroaryl Ethers and N-Benzoyl Heteroarenes.
29528237	1	34	theme	bi	128:129	arg1	s					142:142	C-N-linked bi(heteroaryl)s	117:142	C-N-linked bi(heteroaryl)s	117:142	C-N-linked bi(heteroaryl)s are synthesized by a rhodium-catalyzed N-heteroarylation reaction of N-benzoyl heteroarenes including azoles/azolones, pyridones, cyclic ureas, and cyclic imides using heteroaryl aryl ethers.
29528237	1	35	theme	heteroarenes	223:234	arg1	reaction					201:208	a rhodium-catalyzed N-heteroarylation reaction	163:208	a rhodium-catalyzed N-heteroarylation reaction of N-benzoyl heteroarenes including azoles/azolones, pyridones, cyclic ureas, and cyclic imides using heteroaryl aryl ethers	163:333	C-N-linked bi(heteroaryl)s are synthesized by a rhodium-catalyzed N-heteroarylation reaction of N-benzoyl heteroarenes including azoles/azolones, pyridones, cyclic ureas, and cyclic imides using heteroaryl aryl ethers.
29528237	2	36	theme	diverse	489:495	arg1	s					522:522	diverse C-N-linked bi(heteroaryl)s	489:522	diverse C-N-linked bi(heteroaryl)s containing five- and six-membered heteroarenes	489:569	The reaction involves the covalent bond-exchange reaction of N-CO and HetAr-O bonds without using metal bases and exhibits a broad applicability, giving diverse C-N-linked bi(heteroaryl)s containing five- and six-membered heteroarenes.
29528237	1	37	theme	aryl	323:326	arg1	ethers					328:333	heteroaryl aryl ethers	312:333	heteroaryl aryl ethers	312:333	C-N-linked bi(heteroaryl)s are synthesized by a rhodium-catalyzed N-heteroarylation reaction of N-benzoyl heteroarenes including azoles/azolones, pyridones, cyclic ureas, and cyclic imides using heteroaryl aryl ethers.
29528237	3	38	theme	pyridone	621:628	arg1	N-heteroarylation					576:592	The N-heteroarylation	572:592	The N-heteroarylation of N-H azoles/azolones and pyridone	572:628	The N-heteroarylation of N-H azoles/azolones and pyridone proceeds at higher reaction temperatures.
31695782	6	0	theme	flow	842:845	arg1	cytometry					847:855	flow cytometry	842:855	flow cytometry	842:855	The immune cells of the spleen and colon were analyzed by flow cytometry.
31695782	16	1	theme	receptor-related	2173:2188	arg1	receptor					2197:2204	retinoic acid receptor-related orphan receptor γ	2159:2206	retinoic acid receptor-related orphan receptor γ (RORγt)	2159:2214	Furthermore, TP5 significantly increased the expression level of retinoic acid receptor-related orphan receptor γ (RORγt), a transcription factor for IL-22.
31695782	16	1	theme	receptor-related	2173:2188	arg1	RORγt					2209:2213	RORγt	2209:2213	RORγt	2209:2213	Furthermore, TP5 significantly increased the expression level of retinoic acid receptor-related orphan receptor γ (RORγt), a transcription factor for IL-22.
31695782	16	1	theme	receptor-related	2173:2188	arg1	factor					2233:2238	a transcription factor	2217:2238	a transcription factor for IL-22	2217:2248	Furthermore, TP5 significantly increased the expression level of retinoic acid receptor-related orphan receptor γ (RORγt), a transcription factor for IL-22.
31695782	14	2	theme	critical	1622:1629	arg1	role					1631:1634	the critical role	1618:1634	the critical role of IL-22 in mucosal host defense	1618:1667	Given the critical role of IL-22 in mucosal host defense, we tested the effect of TP5 on mucus barrier and gut microbiota and found that the number of goblet cells and the level of Mucin-2 expression were restored, and the composition of the gut microbiome was normalized after TP5 treatment.
31695782	17	3	with	Consistent	2251:2260	arg1	this					2267:2270	this	2267:2270	this	2267:2270	Consistent with this, RORγt inhibitor abrogated the upregulation of IL-22 induced by TP5.
31695782	15	4	from	role	1918:1921	arg1	effect					1950:1955	the protective effect	1935:1955	the protective effect of TP5 on colitis	1935:1973	The critical role of IL-22 in the protective effect of TP5 on colitis was further confirmed by administering the anti-IL-22 antibody (αIL-22), which completely abolished the effect of TP5.
31695782	15	4	from	role	1918:1921	arg1	colitis					1967:1973	colitis	1967:1973	colitis	1967:1973	The critical role of IL-22 in the protective effect of TP5 on colitis was further confirmed by administering the anti-IL-22 antibody (αIL-22), which completely abolished the effect of TP5.
31695782	4	5	theme	-induced	568:575	arg1	mice					585:588	Dextran sulfate sodium (DSS)-induced colitis mice	540:588	Dextran sulfate sodium (DSS)-induced colitis mice	540:588	Methods: Dextran sulfate sodium (DSS)-induced colitis mice were treated with TP5 by subcutaneous injection.
31695782	11	6	theme	immune	1320:1325	arg1	cells					1327:1331	immune cells	1320:1331	immune cells	1320:1331	TP5 also decreased the infiltration of immune cells and expression levels of pro-inflammatory cytokines such as IL-6.
31695782	16	7	theme	receptor	2197:2204	arg1	level					2150:2154	the expression level	2135:2154	the expression level of retinoic acid receptor-related orphan receptor γ (RORγt), a transcription factor for IL-22	2135:2248	Furthermore, TP5 significantly increased the expression level of retinoic acid receptor-related orphan receptor γ (RORγt), a transcription factor for IL-22.
31695782	18	8	theme	innate	2453:2458	arg1	lymphocytes					2473:2483	both innate and adaptive lymphocytes	2448:2483	both innate and adaptive lymphocytes	2448:2483	Conclusion: TP5 exerts a protective effect on DSS-induced colitis by triggering the production of IL-22 in both innate and adaptive lymphocytes.
31695782	6	9	theme	colon	819:823	arg1	cells					795:799	The immune cells	784:799	The immune cells of the spleen and colon	784:823	The immune cells of the spleen and colon were analyzed by flow cytometry.
31695782	14	10	theme	cells	1770:1774	arg1	number					1753:1758	the number	1749:1758	the number of goblet cells	1749:1774	Given the critical role of IL-22 in mucosal host defense, we tested the effect of TP5 on mucus barrier and gut microbiota and found that the number of goblet cells and the level of Mucin-2 expression were restored, and the composition of the gut microbiome was normalized after TP5 treatment.
31695782	14	10	theme	cells	1770:1774	arg1	level					1784:1788	the level	1780:1788	the level of Mucin-2 expression	1780:1810	Given the critical role of IL-22 in mucosal host defense, we tested the effect of TP5 on mucus barrier and gut microbiota and found that the number of goblet cells and the level of Mucin-2 expression were restored, and the composition of the gut microbiome was normalized after TP5 treatment.
31695782	12	11	from	thymus	1424:1429	arg1	blood					1473:1477	peripheral blood	1462:1477	peripheral blood	1462:1477	Importantly, the damaged thymus and compromised lymphocytes in peripheral blood were significantly restored by TP5.
31695782	6	12	theme	spleen	808:813	arg1	cells					795:799	The immune cells	784:799	The immune cells of the spleen and colon	784:823	The immune cells of the spleen and colon were analyzed by flow cytometry.
31695782	8	13	theme	Bacterial	931:939	arg1	DNA					941:943	Bacterial DNA	931:943	Bacterial DNA	931:943	Bacterial DNA was extracted from mouse colonic feces to assess the intestinal microbiota.
31695782	17	14	theme	RORγt	2273:2277	arg1	inhibitor					2279:2287	RORγt inhibitor	2273:2287	RORγt inhibitor	2273:2287	Consistent with this, RORγt inhibitor abrogated the upregulation of IL-22 induced by TP5.
31695782	2	15	theme	thymic	323:328	arg1	atrophy					330:336	thymic atrophy	323:336	thymic atrophy	323:336	Previous studies have found a positive correlation between thymic atrophy and colitis severity.
31695782	15	16	from	effect	1950:1955	arg1	colitis					1967:1973	colitis	1967:1973	colitis	1967:1973	The critical role of IL-22 in the protective effect of TP5 on colitis was further confirmed by administering the anti-IL-22 antibody (αIL-22), which completely abolished the effect of TP5.
31695782	0	17	theme	adaptive	120:127	arg1	lymphocytes					129:139	both innate and adaptive lymphocytes	104:139	both innate and adaptive lymphocytes	104:139	Thymopentin ameliorates dextran sulfate sodium-induced colitis by triggering the production of IL-22 in both innate and adaptive lymphocytes.
31695782	1	18	theme	inflammatory	191:202	arg1	Background					142:151	Background	142:151	Background: Ulcerative colitis (UC) is a chronic inflammatory gastrointestinal disease, notoriously challenging to treat.	142:262	Background: Ulcerative colitis (UC) is a chronic inflammatory gastrointestinal disease, notoriously challenging to treat.
31695782	1	18	theme	inflammatory	191:202	arg1	colitis					165:171	Ulcerative colitis	154:171	Ulcerative colitis (UC)	154:176	Background: Ulcerative colitis (UC) is a chronic inflammatory gastrointestinal disease, notoriously challenging to treat.
31695782	1	18	theme	inflammatory	191:202	arg1	disease					221:227	a chronic inflammatory gastrointestinal disease	181:227	a chronic inflammatory gastrointestinal disease	181:227	Background: Ulcerative colitis (UC) is a chronic inflammatory gastrointestinal disease, notoriously challenging to treat.
31695782	4	19	theme	subcutaneous	615:626	arg1	injection					628:636	subcutaneous injection	615:636	subcutaneous injection	615:636	Methods: Dextran sulfate sodium (DSS)-induced colitis mice were treated with TP5 by subcutaneous injection.
31695782	18	20	theme	DSS-induced	2387:2397	arg1	colitis					2399:2405	DSS-induced colitis	2387:2405	DSS-induced colitis	2387:2405	Conclusion: TP5 exerts a protective effect on DSS-induced colitis by triggering the production of IL-22 in both innate and adaptive lymphocytes.
31695782	5	21	theme	immune	680:685	arg1	index					693:697	immune organ index	680:697	immune organ index	680:697	Body weight, colon length, colon weight, immune organ index, disease activity index (DAI) score, and the peripheral blood profile were examined.
31695782	18	22	from	production	2425:2434	arg1	lymphocytes					2473:2483	both innate and adaptive lymphocytes	2448:2483	both innate and adaptive lymphocytes	2448:2483	Conclusion: TP5 exerts a protective effect on DSS-induced colitis by triggering the production of IL-22 in both innate and adaptive lymphocytes.
31695782	5	23	theme	blood	755:759	arg1	profile					761:767	the peripheral blood profile	740:767	the peripheral blood profile	740:767	Body weight, colon length, colon weight, immune organ index, disease activity index (DAI) score, and the peripheral blood profile were examined.
31695782	10	24	theme	colon	1193:1197	arg1	length					1199:1204	colon length	1193:1204	colon length	1193:1204	Results: TP5 treatment increased the body weight and colon length, decreased the DAI score, and restored colon architecture of colitic mice.
31695782	15	25	from	colitis	1967:1973	arg1	role					1918:1921	The critical role	1905:1921	The critical role of IL-22 in the protective effect of TP5 on colitis	1905:1973	The critical role of IL-22 in the protective effect of TP5 on colitis was further confirmed by administering the anti-IL-22 antibody (αIL-22), which completely abolished the effect of TP5.
31695782	5	26	theme	colon	652:656	arg1	length					658:663	colon length	652:663	colon length	652:663	Body weight, colon length, colon weight, immune organ index, disease activity index (DAI) score, and the peripheral blood profile were examined.
31695782	10	27	theme	TP5	1149:1151	arg1	treatment					1153:1161	TP5 treatment	1149:1161	TP5 treatment	1149:1161	Results: TP5 treatment increased the body weight and colon length, decreased the DAI score, and restored colon architecture of colitic mice.
31695782	2	28	theme	Previous	264:271	arg1	studies					273:279	Previous studies	264:279	Previous studies	264:279	Previous studies have found a positive correlation between thymic atrophy and colitis severity.
31695782	10	29	theme	DAI	1221:1223	arg1	score					1225:1229	the DAI score	1217:1229	the DAI score	1217:1229	Results: TP5 treatment increased the body weight and colon length, decreased the DAI score, and restored colon architecture of colitic mice.
31695782	12	30	theme	peripheral	1462:1471	arg1	blood					1473:1477	peripheral blood	1462:1477	peripheral blood	1462:1477	Importantly, the damaged thymus and compromised lymphocytes in peripheral blood were significantly restored by TP5.
31695782	11	31	theme	cytokines	1375:1383	arg1	infiltration					1304:1315	the infiltration	1300:1315	the infiltration of immune cells	1300:1331	TP5 also decreased the infiltration of immune cells and expression levels of pro-inflammatory cytokines such as IL-6.
31695782	11	31	theme	cytokines	1375:1383	arg1	levels					1348:1353	expression levels	1337:1353	expression levels of pro-inflammatory cytokines such as IL-6	1337:1396	TP5 also decreased the infiltration of immune cells and expression levels of pro-inflammatory cytokines such as IL-6.
31695782	5	32	theme	index	717:721	arg1	score					729:733	disease activity index (DAI) score	700:733	disease activity index (DAI) score	700:733	Body weight, colon length, colon weight, immune organ index, disease activity index (DAI) score, and the peripheral blood profile were examined.
31695782	14	33	from	effect	1684:1689	arg1	barrier					1707:1713	mucus barrier	1701:1713	mucus barrier	1701:1713	Given the critical role of IL-22 in mucosal host defense, we tested the effect of TP5 on mucus barrier and gut microbiota and found that the number of goblet cells and the level of Mucin-2 expression were restored, and the composition of the gut microbiome was normalized after TP5 treatment.
31695782	14	33	from	effect	1684:1689	arg1	microbiota					1723:1732	gut microbiota	1719:1732	gut microbiota	1719:1732	Given the critical role of IL-22 in mucosal host defense, we tested the effect of TP5 on mucus barrier and gut microbiota and found that the number of goblet cells and the level of Mucin-2 expression were restored, and the composition of the gut microbiome was normalized after TP5 treatment.
31695782	14	34	theme	mucus	1701:1705	arg1	barrier					1707:1713	mucus barrier	1701:1713	mucus barrier	1701:1713	Given the critical role of IL-22 in mucosal host defense, we tested the effect of TP5 on mucus barrier and gut microbiota and found that the number of goblet cells and the level of Mucin-2 expression were restored, and the composition of the gut microbiome was normalized after TP5 treatment.
31695782	10	35	theme	body	1177:1180	arg1	weight					1182:1187	body weight	1177:1187	body weight	1177:1187	Results: TP5 treatment increased the body weight and colon length, decreased the DAI score, and restored colon architecture of colitic mice.
31695782	7	36	theme	colon	894:898	arg1	tissues					900:906	isolated colon tissues	885:906	isolated colon tissues for cytokine analysis	885:928	Histology was performed on isolated colon tissues for cytokine analysis.
31695782	14	37	theme	TP5	1694:1696	arg1	effect					1684:1689	the effect	1680:1689	the effect of TP5 on mucus barrier and gut microbiota	1680:1732	Given the critical role of IL-22 in mucosal host defense, we tested the effect of TP5 on mucus barrier and gut microbiota and found that the number of goblet cells and the level of Mucin-2 expression were restored, and the composition of the gut microbiome was normalized after TP5 treatment.
31695782	8	38	theme	colonic	970:976	arg1	feces					978:982	mouse colonic feces	964:982	mouse colonic feces	964:982	Bacterial DNA was extracted from mouse colonic feces to assess the intestinal microbiota.
31695782	14	39	theme	microbiome	1858:1867	arg1	composition					1835:1845	the composition	1831:1845	the composition of the gut microbiome	1831:1867	Given the critical role of IL-22 in mucosal host defense, we tested the effect of TP5 on mucus barrier and gut microbiota and found that the number of goblet cells and the level of Mucin-2 expression were restored, and the composition of the gut microbiome was normalized after TP5 treatment.
31695782	13	40	theme	lymphoid	1574:1581	arg1	cells					1583:1587	innate and adaptive lymphoid cells	1554:1587	innate and adaptive lymphoid cells	1554:1587	Also, the production of IL-22, both in innate and adaptive lymphoid cells, was triggered by TP5.
31695782	18	41	theme	adaptive	2464:2471	arg1	lymphocytes					2473:2483	both innate and adaptive lymphocytes	2448:2483	both innate and adaptive lymphocytes	2448:2483	Conclusion: TP5 exerts a protective effect on DSS-induced colitis by triggering the production of IL-22 in both innate and adaptive lymphocytes.
31695782	5	42	theme	disease	700:706	arg1	index					717:721	disease activity index	700:721	disease activity index (DAI) score	700:733	Body weight, colon length, colon weight, immune organ index, disease activity index (DAI) score, and the peripheral blood profile were examined.
31695782	5	42	theme	disease	700:706	arg1	DAI					724:726	DAI	724:726	DAI	724:726	Body weight, colon length, colon weight, immune organ index, disease activity index (DAI) score, and the peripheral blood profile were examined.
31695782	12	43	from	lymphocytes	1447:1457	arg1	blood					1473:1477	peripheral blood	1462:1477	peripheral blood	1462:1477	Importantly, the damaged thymus and compromised lymphocytes in peripheral blood were significantly restored by TP5.
31695782	9	44	theme	lamina	1032:1037	arg1	propria					1039:1045	Intestinal lamina propria	1021:1045	Intestinal lamina propria mononuclear cells (LPMCs)	1021:1071	Intestinal lamina propria mononuclear cells (LPMCs), HCT116, CT26, and splenocytes were cultured and treated with TP5.
31695782	5	45	theme	peripheral	744:753	arg1	profile					761:767	the peripheral blood profile	740:767	the peripheral blood profile	740:767	Body weight, colon length, colon weight, immune organ index, disease activity index (DAI) score, and the peripheral blood profile were examined.
31695782	16	46	theme	orphan	2190:2195	arg1	receptor					2197:2204	retinoic acid receptor-related orphan receptor γ	2159:2206	retinoic acid receptor-related orphan receptor γ (RORγt)	2159:2214	Furthermore, TP5 significantly increased the expression level of retinoic acid receptor-related orphan receptor γ (RORγt), a transcription factor for IL-22.
31695782	16	46	theme	orphan	2190:2195	arg1	RORγt					2209:2213	RORγt	2209:2213	RORγt	2209:2213	Furthermore, TP5 significantly increased the expression level of retinoic acid receptor-related orphan receptor γ (RORγt), a transcription factor for IL-22.
31695782	16	46	theme	orphan	2190:2195	arg1	factor					2233:2238	a transcription factor	2217:2238	a transcription factor for IL-22	2217:2248	Furthermore, TP5 significantly increased the expression level of retinoic acid receptor-related orphan receptor γ (RORγt), a transcription factor for IL-22.
31695782	9	47	theme	mononuclear	1047:1057	arg1	LPMCs					1066:1070	LPMCs	1066:1070	LPMCs	1066:1070	Intestinal lamina propria mononuclear cells (LPMCs), HCT116, CT26, and splenocytes were cultured and treated with TP5.
31695782	9	47	theme	mononuclear	1047:1057	arg1	cells					1059:1063	Intestinal lamina propria mononuclear cells	1021:1063	Intestinal lamina propria mononuclear cells (LPMCs)	1021:1071	Intestinal lamina propria mononuclear cells (LPMCs), HCT116, CT26, and splenocytes were cultured and treated with TP5.
31695782	0	48	theme	dextran	24:30	arg1	sulfate					32:38	dextran sulfate	24:38	dextran sulfate sodium-induced colitis	24:61	Thymopentin ameliorates dextran sulfate sodium-induced colitis by triggering the production of IL-22 in both innate and adaptive lymphocytes.
31695782	15	49	theme	TP5	2089:2091	arg1	effect					2079:2084	the effect	2075:2084	the effect of TP5	2075:2091	The critical role of IL-22 in the protective effect of TP5 on colitis was further confirmed by administering the anti-IL-22 antibody (αIL-22), which completely abolished the effect of TP5.
31695782	11	50	theme	pro-inflammatory	1358:1373	arg1	cytokines					1375:1383	pro-inflammatory cytokines	1358:1383	pro-inflammatory cytokines such as IL-6	1358:1396	TP5 also decreased the infiltration of immune cells and expression levels of pro-inflammatory cytokines such as IL-6.
31695782	11	50	theme	pro-inflammatory	1358:1373	arg1	IL-6					1393:1396	IL-6	1393:1396	IL-6	1393:1396	TP5 also decreased the infiltration of immune cells and expression levels of pro-inflammatory cytokines such as IL-6.
31695782	0	51	theme	sodium-induced	40:53	arg1	colitis					55:61	dextran sulfate sodium-induced colitis	24:61	dextran sulfate sodium-induced colitis	24:61	Thymopentin ameliorates dextran sulfate sodium-induced colitis by triggering the production of IL-22 in both innate and adaptive lymphocytes.
31695782	15	52	theme	TP5	1960:1962	arg1	effect					1950:1955	the protective effect	1935:1955	the protective effect of TP5 on colitis	1935:1973	The critical role of IL-22 in the protective effect of TP5 on colitis was further confirmed by administering the anti-IL-22 antibody (αIL-22), which completely abolished the effect of TP5.
31695782	3	53	theme	active	484:489	arg1	domain					491:496	the active domain	480:496	the active domain of the thymopoietin	480:516	It was, therefore, worthwhile to investigate the effect of thymopentin (TP5), a synthetic pentapeptide corresponding to the active domain of the thymopoietin, on colitis.
31695782	3	53	theme	active	484:489	arg1	thymopoietin					505:516	the thymopoietin	501:516	the thymopoietin	501:516	It was, therefore, worthwhile to investigate the effect of thymopentin (TP5), a synthetic pentapeptide corresponding to the active domain of the thymopoietin, on colitis.
31695782	14	54	theme	expression	1801:1810	arg1	number					1753:1758	the number	1749:1758	the number of goblet cells	1749:1774	Given the critical role of IL-22 in mucosal host defense, we tested the effect of TP5 on mucus barrier and gut microbiota and found that the number of goblet cells and the level of Mucin-2 expression were restored, and the composition of the gut microbiome was normalized after TP5 treatment.
31695782	14	54	theme	expression	1801:1810	arg1	level					1784:1788	the level	1780:1788	the level of Mucin-2 expression	1780:1810	Given the critical role of IL-22 in mucosal host defense, we tested the effect of TP5 on mucus barrier and gut microbiota and found that the number of goblet cells and the level of Mucin-2 expression were restored, and the composition of the gut microbiome was normalized after TP5 treatment.
31695782	16	55	theme	transcription	2219:2231	arg1	factor					2233:2238	a transcription factor	2217:2238	a transcription factor for IL-22	2217:2248	Furthermore, TP5 significantly increased the expression level of retinoic acid receptor-related orphan receptor γ (RORγt), a transcription factor for IL-22.
31695782	16	55	theme	transcription	2219:2231	arg1	receptor					2197:2204	retinoic acid receptor-related orphan receptor γ	2159:2206	retinoic acid receptor-related orphan receptor γ (RORγt)	2159:2214	Furthermore, TP5 significantly increased the expression level of retinoic acid receptor-related orphan receptor γ (RORγt), a transcription factor for IL-22.
31695782	2	56	theme	colitis	342:348	arg1	severity					350:357	colitis severity	342:357	colitis severity	342:357	Previous studies have found a positive correlation between thymic atrophy and colitis severity.
31695782	14	57	theme	mucosal	1648:1654	arg1	defense					1661:1667	mucosal host defense	1648:1667	mucosal host defense	1648:1667	Given the critical role of IL-22 in mucosal host defense, we tested the effect of TP5 on mucus barrier and gut microbiota and found that the number of goblet cells and the level of Mucin-2 expression were restored, and the composition of the gut microbiome was normalized after TP5 treatment.
31695782	1	58	theme	Ulcerative	154:163	arg1	UC					174:175	UC	174:175	UC	174:175	Background: Ulcerative colitis (UC) is a chronic inflammatory gastrointestinal disease, notoriously challenging to treat.
31695782	1	58	theme	Ulcerative	154:163	arg1	Background					142:151	Background	142:151	Background: Ulcerative colitis (UC) is a chronic inflammatory gastrointestinal disease, notoriously challenging to treat.	142:262	Background: Ulcerative colitis (UC) is a chronic inflammatory gastrointestinal disease, notoriously challenging to treat.
31695782	1	58	theme	Ulcerative	154:163	arg1	colitis					165:171	Ulcerative colitis	154:171	Ulcerative colitis (UC)	154:176	Background: Ulcerative colitis (UC) is a chronic inflammatory gastrointestinal disease, notoriously challenging to treat.
31695782	1	58	theme	Ulcerative	154:163	arg1	disease					221:227	a chronic inflammatory gastrointestinal disease	181:227	a chronic inflammatory gastrointestinal disease	181:227	Background: Ulcerative colitis (UC) is a chronic inflammatory gastrointestinal disease, notoriously challenging to treat.
31695782	4	59	theme	colitis	577:583	arg1	mice					585:588	Dextran sulfate sodium (DSS)-induced colitis mice	540:588	Dextran sulfate sodium (DSS)-induced colitis mice	540:588	Methods: Dextran sulfate sodium (DSS)-induced colitis mice were treated with TP5 by subcutaneous injection.
31695782	3	60	theme	synthetic	440:448	arg1	pentapeptide					450:461	a synthetic pentapeptide	438:461	a synthetic pentapeptide corresponding to the active domain of the thymopoietin	438:516	It was, therefore, worthwhile to investigate the effect of thymopentin (TP5), a synthetic pentapeptide corresponding to the active domain of the thymopoietin, on colitis.
31695782	3	60	theme	synthetic	440:448	arg1	thymopentin					419:429	thymopentin	419:429	thymopentin (TP5)	419:435	It was, therefore, worthwhile to investigate the effect of thymopentin (TP5), a synthetic pentapeptide corresponding to the active domain of the thymopoietin, on colitis.
31695782	10	61	theme	colitic	1267:1273	arg1	mice					1275:1278	colitic mice	1267:1278	colitic mice	1267:1278	Results: TP5 treatment increased the body weight and colon length, decreased the DAI score, and restored colon architecture of colitic mice.
31695782	3	62	theme	thymopoietin	505:516	arg1	domain					491:496	the active domain	480:496	the active domain of the thymopoietin	480:516	It was, therefore, worthwhile to investigate the effect of thymopentin (TP5), a synthetic pentapeptide corresponding to the active domain of the thymopoietin, on colitis.
31695782	3	62	theme	thymopoietin	505:516	arg1	thymopoietin					505:516	the thymopoietin	501:516	the thymopoietin	501:516	It was, therefore, worthwhile to investigate the effect of thymopentin (TP5), a synthetic pentapeptide corresponding to the active domain of the thymopoietin, on colitis.
31695782	14	63	theme	IL-22	1639:1643	arg1	role					1631:1634	the critical role	1618:1634	the critical role of IL-22 in mucosal host defense	1618:1667	Given the critical role of IL-22 in mucosal host defense, we tested the effect of TP5 on mucus barrier and gut microbiota and found that the number of goblet cells and the level of Mucin-2 expression were restored, and the composition of the gut microbiome was normalized after TP5 treatment.
31695782	15	64	theme	IL-22	1926:1930	arg1	role					1918:1921	The critical role	1905:1921	The critical role of IL-22 in the protective effect of TP5 on colitis	1905:1973	The critical role of IL-22 in the protective effect of TP5 on colitis was further confirmed by administering the anti-IL-22 antibody (αIL-22), which completely abolished the effect of TP5.
31695782	6	65	theme	immune	788:793	arg1	cells					795:799	The immune cells	784:799	The immune cells of the spleen and colon	784:823	The immune cells of the spleen and colon were analyzed by flow cytometry.
31695782	5	66	theme	Body	639:642	arg1	weight					644:649	Body weight	639:649	Body weight	639:649	Body weight, colon length, colon weight, immune organ index, disease activity index (DAI) score, and the peripheral blood profile were examined.
31695782	16	67	theme	acid	2168:2171	arg1	receptor					2197:2204	retinoic acid receptor-related orphan receptor γ	2159:2206	retinoic acid receptor-related orphan receptor γ (RORγt)	2159:2214	Furthermore, TP5 significantly increased the expression level of retinoic acid receptor-related orphan receptor γ (RORγt), a transcription factor for IL-22.
31695782	16	67	theme	acid	2168:2171	arg1	RORγt					2209:2213	RORγt	2209:2213	RORγt	2209:2213	Furthermore, TP5 significantly increased the expression level of retinoic acid receptor-related orphan receptor γ (RORγt), a transcription factor for IL-22.
31695782	16	67	theme	acid	2168:2171	arg1	factor					2233:2238	a transcription factor	2217:2238	a transcription factor for IL-22	2217:2248	Furthermore, TP5 significantly increased the expression level of retinoic acid receptor-related orphan receptor γ (RORγt), a transcription factor for IL-22.
31695782	2	68	theme	positive	294:301	arg1	correlation					303:313	a positive correlation	292:313	a positive correlation between thymic atrophy and colitis severity	292:357	Previous studies have found a positive correlation between thymic atrophy and colitis severity.
31695782	18	69	theme	IL-22	2439:2443	arg1	production					2425:2434	the production	2421:2434	the production of IL-22 in both innate and adaptive lymphocytes	2421:2483	Conclusion: TP5 exerts a protective effect on DSS-induced colitis by triggering the production of IL-22 in both innate and adaptive lymphocytes.
31695782	16	70	theme	expression	2139:2148	arg1	level					2150:2154	the expression level	2135:2154	the expression level of retinoic acid receptor-related orphan receptor γ (RORγt), a transcription factor for IL-22	2135:2248	Furthermore, TP5 significantly increased the expression level of retinoic acid receptor-related orphan receptor γ (RORγt), a transcription factor for IL-22.
31695782	19	71	theme	potential	2548:2556	arg1	TP5					2508:2510	TP5	2508:2510	TP5	2508:2510	This study delineates TP5 as an immunomodulator that may be a potential drug for the treatment of UC.
31695782	19	71	theme	potential	2548:2556	arg1	immunomodulator					2518:2532	an immunomodulator	2515:2532	an immunomodulator that may be a potential drug for the treatment of UC	2515:2585	This study delineates TP5 as an immunomodulator that may be a potential drug for the treatment of UC.
31695782	19	71	theme	potential	2548:2556	arg1	drug					2558:2561	a potential drug	2546:2561	a potential drug for the treatment of UC	2546:2585	This study delineates TP5 as an immunomodulator that may be a potential drug for the treatment of UC.
31695782	18	72	theme	protective	2366:2375	arg1	effect					2377:2382	a protective effect	2364:2382	a protective effect on DSS-induced colitis	2364:2405	Conclusion: TP5 exerts a protective effect on DSS-induced colitis by triggering the production of IL-22 in both innate and adaptive lymphocytes.
31695782	13	73	theme	IL-22	1539:1543	arg1	production					1525:1534	the production	1521:1534	the production of IL-22, both in innate and adaptive lymphoid cells,	1521:1588	Also, the production of IL-22, both in innate and adaptive lymphoid cells, was triggered by TP5.
31695782	14	74	theme	goblet	1763:1768	arg1	cells					1770:1774	goblet cells	1763:1774	goblet cells	1763:1774	Given the critical role of IL-22 in mucosal host defense, we tested the effect of TP5 on mucus barrier and gut microbiota and found that the number of goblet cells and the level of Mucin-2 expression were restored, and the composition of the gut microbiome was normalized after TP5 treatment.
31695782	5	75	theme	colon	666:670	arg1	weight					672:677	colon weight	666:677	colon weight	666:677	Body weight, colon length, colon weight, immune organ index, disease activity index (DAI) score, and the peripheral blood profile were examined.
31695782	1	76	theme	chronic	183:189	arg1	Background					142:151	Background	142:151	Background: Ulcerative colitis (UC) is a chronic inflammatory gastrointestinal disease, notoriously challenging to treat.	142:262	Background: Ulcerative colitis (UC) is a chronic inflammatory gastrointestinal disease, notoriously challenging to treat.
31695782	1	76	theme	chronic	183:189	arg1	colitis					165:171	Ulcerative colitis	154:171	Ulcerative colitis (UC)	154:176	Background: Ulcerative colitis (UC) is a chronic inflammatory gastrointestinal disease, notoriously challenging to treat.
31695782	1	76	theme	chronic	183:189	arg1	disease					221:227	a chronic inflammatory gastrointestinal disease	181:227	a chronic inflammatory gastrointestinal disease	181:227	Background: Ulcerative colitis (UC) is a chronic inflammatory gastrointestinal disease, notoriously challenging to treat.
31695782	1	77	theme	gastrointestinal	204:219	arg1	Background					142:151	Background	142:151	Background: Ulcerative colitis (UC) is a chronic inflammatory gastrointestinal disease, notoriously challenging to treat.	142:262	Background: Ulcerative colitis (UC) is a chronic inflammatory gastrointestinal disease, notoriously challenging to treat.
31695782	1	77	theme	gastrointestinal	204:219	arg1	colitis					165:171	Ulcerative colitis	154:171	Ulcerative colitis (UC)	154:176	Background: Ulcerative colitis (UC) is a chronic inflammatory gastrointestinal disease, notoriously challenging to treat.
31695782	1	77	theme	gastrointestinal	204:219	arg1	disease					221:227	a chronic inflammatory gastrointestinal disease	181:227	a chronic inflammatory gastrointestinal disease	181:227	Background: Ulcerative colitis (UC) is a chronic inflammatory gastrointestinal disease, notoriously challenging to treat.
31695782	0	78	from	production	81:90	arg1	lymphocytes					129:139	both innate and adaptive lymphocytes	104:139	both innate and adaptive lymphocytes	104:139	Thymopentin ameliorates dextran sulfate sodium-induced colitis by triggering the production of IL-22 in both innate and adaptive lymphocytes.
31695782	14	79	theme	gut	1719:1721	arg1	microbiota					1723:1732	gut microbiota	1719:1732	gut microbiota	1719:1732	Given the critical role of IL-22 in mucosal host defense, we tested the effect of TP5 on mucus barrier and gut microbiota and found that the number of goblet cells and the level of Mucin-2 expression were restored, and the composition of the gut microbiome was normalized after TP5 treatment.
31695782	5	80	theme	activity	708:715	arg1	index					717:721	disease activity index	700:721	disease activity index (DAI) score	700:733	Body weight, colon length, colon weight, immune organ index, disease activity index (DAI) score, and the peripheral blood profile were examined.
31695782	5	80	theme	activity	708:715	arg1	DAI					724:726	DAI	724:726	DAI	724:726	Body weight, colon length, colon weight, immune organ index, disease activity index (DAI) score, and the peripheral blood profile were examined.
31695782	19	81	theme	UC	2584:2585	arg1	treatment					2571:2579	the treatment	2567:2579	the treatment of UC	2567:2585	This study delineates TP5 as an immunomodulator that may be a potential drug for the treatment of UC.
31695782	0	82	theme	IL-22	95:99	arg1	production					81:90	the production	77:90	the production of IL-22 in both innate and adaptive lymphocytes	77:139	Thymopentin ameliorates dextran sulfate sodium-induced colitis by triggering the production of IL-22 in both innate and adaptive lymphocytes.
31695782	3	83	from	effect	409:414	arg1	colitis					522:528	colitis	522:528	colitis	522:528	It was, therefore, worthwhile to investigate the effect of thymopentin (TP5), a synthetic pentapeptide corresponding to the active domain of the thymopoietin, on colitis.
31695782	8	84	theme	mouse	964:968	arg1	feces					978:982	mouse colonic feces	964:982	mouse colonic feces	964:982	Bacterial DNA was extracted from mouse colonic feces to assess the intestinal microbiota.
31695782	7	85	theme	cytokine	912:919	arg1	analysis					921:928	cytokine analysis	912:928	cytokine analysis	912:928	Histology was performed on isolated colon tissues for cytokine analysis.
31695782	15	86	theme	anti-IL-22	2018:2027	arg1	αIL-22					2039:2044	αIL-22	2039:2044	αIL-22	2039:2044	The critical role of IL-22 in the protective effect of TP5 on colitis was further confirmed by administering the anti-IL-22 antibody (αIL-22), which completely abolished the effect of TP5.
31695782	15	86	theme	anti-IL-22	2018:2027	arg1	antibody					2029:2036	the anti-IL-22 antibody	2014:2036	the anti-IL-22 antibody (αIL-22)	2014:2045	The critical role of IL-22 in the protective effect of TP5 on colitis was further confirmed by administering the anti-IL-22 antibody (αIL-22), which completely abolished the effect of TP5.
31695782	12	87	theme	compromised	1435:1445	arg1	lymphocytes					1447:1457	compromised lymphocytes	1435:1457	compromised lymphocytes	1435:1457	Importantly, the damaged thymus and compromised lymphocytes in peripheral blood were significantly restored by TP5.
31695782	16	88	theme	retinoic	2159:2166	arg1	receptor					2197:2204	retinoic acid receptor-related orphan receptor γ	2159:2206	retinoic acid receptor-related orphan receptor γ (RORγt)	2159:2214	Furthermore, TP5 significantly increased the expression level of retinoic acid receptor-related orphan receptor γ (RORγt), a transcription factor for IL-22.
31695782	16	88	theme	retinoic	2159:2166	arg1	RORγt					2209:2213	RORγt	2209:2213	RORγt	2209:2213	Furthermore, TP5 significantly increased the expression level of retinoic acid receptor-related orphan receptor γ (RORγt), a transcription factor for IL-22.
31695782	16	88	theme	retinoic	2159:2166	arg1	factor					2233:2238	a transcription factor	2217:2238	a transcription factor for IL-22	2217:2248	Furthermore, TP5 significantly increased the expression level of retinoic acid receptor-related orphan receptor γ (RORγt), a transcription factor for IL-22.
31695782	5	89	theme	organ	687:691	arg1	index					693:697	immune organ index	680:697	immune organ index	680:697	Body weight, colon length, colon weight, immune organ index, disease activity index (DAI) score, and the peripheral blood profile were examined.
31695782	15	90	theme	critical	1909:1916	arg1	role					1918:1921	The critical role	1905:1921	The critical role of IL-22 in the protective effect of TP5 on colitis	1905:1973	The critical role of IL-22 in the protective effect of TP5 on colitis was further confirmed by administering the anti-IL-22 antibody (αIL-22), which completely abolished the effect of TP5.
31695782	11	91	theme	expression	1337:1346	arg1	levels					1348:1353	expression levels	1337:1353	expression levels of pro-inflammatory cytokines such as IL-6	1337:1396	TP5 also decreased the infiltration of immune cells and expression levels of pro-inflammatory cytokines such as IL-6.
31695782	14	92	from	role	1631:1634	arg1	defense					1661:1667	mucosal host defense	1648:1667	mucosal host defense	1648:1667	Given the critical role of IL-22 in mucosal host defense, we tested the effect of TP5 on mucus barrier and gut microbiota and found that the number of goblet cells and the level of Mucin-2 expression were restored, and the composition of the gut microbiome was normalized after TP5 treatment.
31695782	18	93	from	effect	2377:2382	arg1	colitis					2399:2405	DSS-induced colitis	2387:2405	DSS-induced colitis	2387:2405	Conclusion: TP5 exerts a protective effect on DSS-induced colitis by triggering the production of IL-22 in both innate and adaptive lymphocytes.
31695782	7	94	theme	isolated	885:892	arg1	tissues					900:906	isolated colon tissues	885:906	isolated colon tissues for cytokine analysis	885:928	Histology was performed on isolated colon tissues for cytokine analysis.
31695782	14	95	theme	gut	1854:1856	arg1	microbiome					1858:1867	the gut microbiome	1850:1867	the gut microbiome	1850:1867	Given the critical role of IL-22 in mucosal host defense, we tested the effect of TP5 on mucus barrier and gut microbiota and found that the number of goblet cells and the level of Mucin-2 expression were restored, and the composition of the gut microbiome was normalized after TP5 treatment.
31695782	9	96	theme	Intestinal	1021:1030	arg1	propria					1039:1045	Intestinal lamina propria	1021:1045	Intestinal lamina propria mononuclear cells (LPMCs)	1021:1071	Intestinal lamina propria mononuclear cells (LPMCs), HCT116, CT26, and splenocytes were cultured and treated with TP5.
31695782	8	97	theme	intestinal	998:1007	arg1	microbiota					1009:1018	the intestinal microbiota	994:1018	the intestinal microbiota	994:1018	Bacterial DNA was extracted from mouse colonic feces to assess the intestinal microbiota.
31695782	9	98	theme	propria	1039:1045	arg1	LPMCs					1066:1070	LPMCs	1066:1070	LPMCs	1066:1070	Intestinal lamina propria mononuclear cells (LPMCs), HCT116, CT26, and splenocytes were cultured and treated with TP5.
31695782	9	98	theme	propria	1039:1045	arg1	cells					1059:1063	Intestinal lamina propria mononuclear cells	1021:1063	Intestinal lamina propria mononuclear cells (LPMCs)	1021:1071	Intestinal lamina propria mononuclear cells (LPMCs), HCT116, CT26, and splenocytes were cultured and treated with TP5.
31695782	0	99	theme	sulfate	32:38	arg1	colitis					55:61	dextran sulfate sodium-induced colitis	24:61	dextran sulfate sodium-induced colitis	24:61	Thymopentin ameliorates dextran sulfate sodium-induced colitis by triggering the production of IL-22 in both innate and adaptive lymphocytes.
31695782	13	100	theme	innate	1554:1559	arg1	cells					1583:1587	innate and adaptive lymphoid cells	1554:1587	innate and adaptive lymphoid cells	1554:1587	Also, the production of IL-22, both in innate and adaptive lymphoid cells, was triggered by TP5.
31695782	3	101	theme	thymopentin	419:429	arg1	effect					409:414	the effect	405:414	the effect of thymopentin (TP5), a synthetic pentapeptide corresponding to the active domain of the thymopoietin, on colitis	405:528	It was, therefore, worthwhile to investigate the effect of thymopentin (TP5), a synthetic pentapeptide corresponding to the active domain of the thymopoietin, on colitis.
31695782	13	102	theme	adaptive	1565:1572	arg1	cells					1583:1587	innate and adaptive lymphoid cells	1554:1587	innate and adaptive lymphoid cells	1554:1587	Also, the production of IL-22, both in innate and adaptive lymphoid cells, was triggered by TP5.
31695782	10	103	theme	colon	1245:1249	arg1	architecture					1251:1262	colon architecture	1245:1262	colon architecture of colitic mice	1245:1278	Results: TP5 treatment increased the body weight and colon length, decreased the DAI score, and restored colon architecture of colitic mice.
31695782	0	104	theme	innate	109:114	arg1	lymphocytes					129:139	both innate and adaptive lymphocytes	104:139	both innate and adaptive lymphocytes	104:139	Thymopentin ameliorates dextran sulfate sodium-induced colitis by triggering the production of IL-22 in both innate and adaptive lymphocytes.
31695782	12	105	theme	damaged	1416:1422	arg1	thymus					1424:1429	the damaged thymus	1412:1429	the damaged thymus	1412:1429	Importantly, the damaged thymus and compromised lymphocytes in peripheral blood were significantly restored by TP5.
31695782	15	106	theme	protective	1939:1948	arg1	effect					1950:1955	the protective effect	1935:1955	the protective effect of TP5 on colitis	1935:1973	The critical role of IL-22 in the protective effect of TP5 on colitis was further confirmed by administering the anti-IL-22 antibody (αIL-22), which completely abolished the effect of TP5.
31695782	14	107	theme	TP5	1890:1892	arg1	treatment					1894:1902	TP5 treatment	1890:1902	TP5 treatment	1890:1902	Given the critical role of IL-22 in mucosal host defense, we tested the effect of TP5 on mucus barrier and gut microbiota and found that the number of goblet cells and the level of Mucin-2 expression were restored, and the composition of the gut microbiome was normalized after TP5 treatment.
31695782	14	108	theme	host	1656:1659	arg1	defense					1661:1667	mucosal host defense	1648:1667	mucosal host defense	1648:1667	Given the critical role of IL-22 in mucosal host defense, we tested the effect of TP5 on mucus barrier and gut microbiota and found that the number of goblet cells and the level of Mucin-2 expression were restored, and the composition of the gut microbiome was normalized after TP5 treatment.
31695782	14	109	theme	Mucin-2	1793:1799	arg1	expression					1801:1810	Mucin-2 expression	1793:1810	Mucin-2 expression	1793:1810	Given the critical role of IL-22 in mucosal host defense, we tested the effect of TP5 on mucus barrier and gut microbiota and found that the number of goblet cells and the level of Mucin-2 expression were restored, and the composition of the gut microbiome was normalized after TP5 treatment.
31695782	17	110	theme	IL-22	2319:2323	arg1	upregulation					2303:2314	the upregulation	2299:2314	the upregulation of IL-22 induced by TP5	2299:2338	Consistent with this, RORγt inhibitor abrogated the upregulation of IL-22 induced by TP5.
31695782	10	111	theme	mice	1275:1278	arg1	architecture					1251:1262	colon architecture	1245:1262	colon architecture of colitic mice	1245:1278	Results: TP5 treatment increased the body weight and colon length, decreased the DAI score, and restored colon architecture of colitic mice.
31695782	11	112	theme	cells	1327:1331	arg1	infiltration					1304:1315	the infiltration	1300:1315	the infiltration of immune cells	1300:1331	TP5 also decreased the infiltration of immune cells and expression levels of pro-inflammatory cytokines such as IL-6.
31695782	11	112	theme	cells	1327:1331	arg1	levels					1348:1353	expression levels	1337:1353	expression levels of pro-inflammatory cytokines such as IL-6	1337:1396	TP5 also decreased the infiltration of immune cells and expression levels of pro-inflammatory cytokines such as IL-6.
29975908	3	0	theme	nanostructured	508:521	arg1	model					583:587	a drug model	576:587	a drug model	576:587	We combined bacterial cellulose hydrogel (BC) and nanostructured lipid carriers (NLCs) including doxorubicin (Dox) as a drug model.
29975908	3	0	theme	nanostructured	508:521	arg1	NLCs					539:542	NLCs	539:542	NLCs	539:542	We combined bacterial cellulose hydrogel (BC) and nanostructured lipid carriers (NLCs) including doxorubicin (Dox) as a drug model.
29975908	3	0	theme	nanostructured	508:521	arg1	carriers					529:536	nanostructured lipid carriers	508:536	nanostructured lipid carriers (NLCs)	508:543	We combined bacterial cellulose hydrogel (BC) and nanostructured lipid carriers (NLCs) including doxorubicin (Dox) as a drug model.
29975908	3	0	theme	nanostructured	508:521	arg1	hydrogel					490:497	bacterial cellulose hydrogel	470:497	bacterial cellulose hydrogel (BC)	470:502	We combined bacterial cellulose hydrogel (BC) and nanostructured lipid carriers (NLCs) including doxorubicin (Dox) as a drug model.
29975908	5	1	theme	cancer	909:914	arg1	model					922:926	an orthotopic breast cancer mouse model	888:926	an orthotopic breast cancer mouse model	888:926	Thereafter, a fixed combination of NLCs-H and NLCs-N loaded into BC (BC-NLCs-NH) was assayed in vivo into an orthotopic breast cancer mouse model.
29975908	3	2	theme	lipid	523:527	arg1	model					583:587	a drug model	576:587	a drug model	576:587	We combined bacterial cellulose hydrogel (BC) and nanostructured lipid carriers (NLCs) including doxorubicin (Dox) as a drug model.
29975908	3	2	theme	lipid	523:527	arg1	NLCs					539:542	NLCs	539:542	NLCs	539:542	We combined bacterial cellulose hydrogel (BC) and nanostructured lipid carriers (NLCs) including doxorubicin (Dox) as a drug model.
29975908	3	2	theme	lipid	523:527	arg1	carriers					529:536	nanostructured lipid carriers	508:536	nanostructured lipid carriers (NLCs)	508:543	We combined bacterial cellulose hydrogel (BC) and nanostructured lipid carriers (NLCs) including doxorubicin (Dox) as a drug model.
29975908	3	2	theme	lipid	523:527	arg1	hydrogel					490:497	bacterial cellulose hydrogel	470:497	bacterial cellulose hydrogel (BC)	470:502	We combined bacterial cellulose hydrogel (BC) and nanostructured lipid carriers (NLCs) including doxorubicin (Dox) as a drug model.
29975908	8	3	theme	drug	1275:1278	arg1	release					1280:1286	the differential drug release	1258:1286	the differential drug release	1258:1286	Taking advantage of the differential drug release, a mixture of NLCs-N and NLCs-H was encapsulated into BC matrix (BC-NLCs-NH) and assayed in vivo, showing a significant reduction of tumor growth, metastasis incidence and local drug toxicities.
29975908	6	4	theme	encapsulation	947:959	arg1	efficiency					961:970	low encapsulation efficiency	943:970	low encapsulation efficiency (48%)	943:976	NLCs-H showed low encapsulation efficiency (48%) and fast release of the drug while NLCs-N showed higher encapsulation (97%) and sustained drug release.
29975908	6	4	theme	encapsulation	947:959	arg1	%					975:975	48%	973:975	48%	973:975	NLCs-H showed low encapsulation efficiency (48%) and fast release of the drug while NLCs-N showed higher encapsulation (97%) and sustained drug release.
29975908	3	5	theme	bacterial	470:478	arg1	model					583:587	a drug model	576:587	a drug model	576:587	We combined bacterial cellulose hydrogel (BC) and nanostructured lipid carriers (NLCs) including doxorubicin (Dox) as a drug model.
29975908	3	5	theme	bacterial	470:478	arg1	carriers					529:536	nanostructured lipid carriers	508:536	nanostructured lipid carriers (NLCs)	508:543	We combined bacterial cellulose hydrogel (BC) and nanostructured lipid carriers (NLCs) including doxorubicin (Dox) as a drug model.
29975908	3	5	theme	bacterial	470:478	arg1	BC					500:501	BC	500:501	BC	500:501	We combined bacterial cellulose hydrogel (BC) and nanostructured lipid carriers (NLCs) including doxorubicin (Dox) as a drug model.
29975908	3	5	theme	bacterial	470:478	arg1	hydrogel					490:497	bacterial cellulose hydrogel	470:497	bacterial cellulose hydrogel (BC)	470:502	We combined bacterial cellulose hydrogel (BC) and nanostructured lipid carriers (NLCs) including doxorubicin (Dox) as a drug model.
29975908	7	6	theme	free	1228:1231	arg1	Dox					1233:1235	free Dox	1228:1235	free Dox	1228:1235	Both NLCs internalized via endocytic pathway, while allowing a sustained release of the Dox, which in turn rendered IC50 values below of those of free Dox.
29975908	5	7	theme	mouse	916:920	arg1	model					922:926	an orthotopic breast cancer mouse model	888:926	an orthotopic breast cancer mouse model	888:926	Thereafter, a fixed combination of NLCs-H and NLCs-N loaded into BC (BC-NLCs-NH) was assayed in vivo into an orthotopic breast cancer mouse model.
29975908	4	8	theme	cytotoxic	713:721	arg1	efficacy					723:730	cytotoxic efficacy	713:730	cytotoxic efficacy	713:730	NLCs loaded with cationic Dox (NLCs-H) or neutral Dox (NLCs-N) were fully characterized and their cell internalization and cytotoxic efficacy were evaluated in vitro against MDA-MB-231 cells.
29975908	1	9	theme	primary	255:261	arg1	tumors					263:268	primary tumors	255:268	primary tumors prior or after surgery	255:291	The use of hybrid materials, where a matrix sustains nanoparticles controlling the release of the chemotherapeutic drug, could be beneficial for the treatment of primary tumors prior or after surgery.
29975908	6	10	theme	higher	1027:1032	arg1	encapsulation					1034:1046	higher encapsulation	1027:1046	higher encapsulation (97%)	1027:1052	NLCs-H showed low encapsulation efficiency (48%) and fast release of the drug while NLCs-N showed higher encapsulation (97%) and sustained drug release.
29975908	6	10	theme	higher	1027:1032	arg1	%					1051:1051	97%	1049:1051	97%	1049:1051	NLCs-H showed low encapsulation efficiency (48%) and fast release of the drug while NLCs-N showed higher encapsulation (97%) and sustained drug release.
29975908	1	11	theme	tumors	263:268	arg1	treatment					242:250	the treatment	238:250	the treatment of primary tumors prior or after surgery	238:291	The use of hybrid materials, where a matrix sustains nanoparticles controlling the release of the chemotherapeutic drug, could be beneficial for the treatment of primary tumors prior or after surgery.
29975908	7	12	theme	endocytic	1109:1117	arg1	pathway					1119:1125	endocytic pathway	1109:1125	endocytic pathway	1109:1125	Both NLCs internalized via endocytic pathway, while allowing a sustained release of the Dox, which in turn rendered IC50 values below of those of free Dox.
29975908	2	13	theme	drug	343:346	arg1	concentrations					348:361	high drug concentrations	338:361	high drug concentrations	338:361	This localized chemotherapy would guarantee high drug concentrations at the tumor site while precluding systemic drug exposure minimizing undesirable side effects.
29975908	2	14	theme	systemic	398:405	arg1	exposure					412:419	systemic drug exposure	398:419	systemic drug exposure	398:419	This localized chemotherapy would guarantee high drug concentrations at the tumor site while precluding systemic drug exposure minimizing undesirable side effects.
29975908	0	15	theme	cellulose	10:18	arg1	hydrogel					20:27	Bacterial cellulose hydrogel	0:27	Bacterial cellulose hydrogel	0:27	Bacterial cellulose hydrogel loaded with lipid nanoparticles for localized cancer treatment.
29975908	8	16	theme	significant	1396:1406	arg1	reduction					1408:1416	a significant reduction	1394:1416	a significant reduction of tumor growth, metastasis incidence and local drug toxicities	1394:1480	Taking advantage of the differential drug release, a mixture of NLCs-N and NLCs-H was encapsulated into BC matrix (BC-NLCs-NH) and assayed in vivo, showing a significant reduction of tumor growth, metastasis incidence and local drug toxicities.
29975908	7	17	theme	IC50	1198:1201	arg1	values					1203:1208	IC50 values	1198:1208	IC50 values	1198:1208	Both NLCs internalized via endocytic pathway, while allowing a sustained release of the Dox, which in turn rendered IC50 values below of those of free Dox.
29975908	7	18	theme	sustained	1145:1153	arg1	release					1155:1161	a sustained release	1143:1161	a sustained release of the Dox, which in turn rendered IC50 values below of those of free Dox	1143:1235	Both NLCs internalized via endocytic pathway, while allowing a sustained release of the Dox, which in turn rendered IC50 values below of those of free Dox.
29975908	8	19	theme	differential	1262:1273	arg1	release					1280:1286	the differential drug release	1258:1286	the differential drug release	1258:1286	Taking advantage of the differential drug release, a mixture of NLCs-N and NLCs-H was encapsulated into BC matrix (BC-NLCs-NH) and assayed in vivo, showing a significant reduction of tumor growth, metastasis incidence and local drug toxicities.
29975908	6	20	theme	drug	1002:1005	arg1	release					987:993	fast release	982:993	fast release of the drug	982:1005	NLCs-H showed low encapsulation efficiency (48%) and fast release of the drug while NLCs-N showed higher encapsulation (97%) and sustained drug release.
29975908	6	20	theme	drug	1002:1005	arg1	efficiency					961:970	low encapsulation efficiency	943:970	low encapsulation efficiency (48%)	943:976	NLCs-H showed low encapsulation efficiency (48%) and fast release of the drug while NLCs-N showed higher encapsulation (97%) and sustained drug release.
29975908	6	20	theme	drug	1002:1005	arg1	%					975:975	48%	973:975	48%	973:975	NLCs-H showed low encapsulation efficiency (48%) and fast release of the drug while NLCs-N showed higher encapsulation (97%) and sustained drug release.
29975908	0	21	theme	Bacterial	0:8	arg1	hydrogel					20:27	Bacterial cellulose hydrogel	0:27	Bacterial cellulose hydrogel	0:27	Bacterial cellulose hydrogel loaded with lipid nanoparticles for localized cancer treatment.
29975908	8	22	theme	tumor	1421:1425	arg1	growth					1427:1432	tumor growth	1421:1432	tumor growth	1421:1432	Taking advantage of the differential drug release, a mixture of NLCs-N and NLCs-H was encapsulated into BC matrix (BC-NLCs-NH) and assayed in vivo, showing a significant reduction of tumor growth, metastasis incidence and local drug toxicities.
29975908	5	23	theme	NLCs-N	828:833	arg1	combination					802:812	a fixed combination	794:812	a fixed combination of NLCs-H and NLCs-N loaded into BC (BC-NLCs-NH)	794:861	Thereafter, a fixed combination of NLCs-H and NLCs-N loaded into BC (BC-NLCs-NH) was assayed in vivo into an orthotopic breast cancer mouse model.
29975908	2	24	theme	localized	299:307	arg1	chemotherapy					309:320	This localized chemotherapy	294:320	This localized chemotherapy	294:320	This localized chemotherapy would guarantee high drug concentrations at the tumor site while precluding systemic drug exposure minimizing undesirable side effects.
29975908	7	25	theme	Dox	1170:1172	arg1	release					1155:1161	a sustained release	1143:1161	a sustained release of the Dox, which in turn rendered IC50 values below of those of free Dox	1143:1235	Both NLCs internalized via endocytic pathway, while allowing a sustained release of the Dox, which in turn rendered IC50 values below of those of free Dox.
29975908	5	26	theme	breast	902:907	arg1	model					922:926	an orthotopic breast cancer mouse model	888:926	an orthotopic breast cancer mouse model	888:926	Thereafter, a fixed combination of NLCs-H and NLCs-N loaded into BC (BC-NLCs-NH) was assayed in vivo into an orthotopic breast cancer mouse model.
29975908	3	27	theme	cellulose	480:488	arg1	model					583:587	a drug model	576:587	a drug model	576:587	We combined bacterial cellulose hydrogel (BC) and nanostructured lipid carriers (NLCs) including doxorubicin (Dox) as a drug model.
29975908	3	27	theme	cellulose	480:488	arg1	carriers					529:536	nanostructured lipid carriers	508:536	nanostructured lipid carriers (NLCs)	508:543	We combined bacterial cellulose hydrogel (BC) and nanostructured lipid carriers (NLCs) including doxorubicin (Dox) as a drug model.
29975908	3	27	theme	cellulose	480:488	arg1	BC					500:501	BC	500:501	BC	500:501	We combined bacterial cellulose hydrogel (BC) and nanostructured lipid carriers (NLCs) including doxorubicin (Dox) as a drug model.
29975908	3	27	theme	cellulose	480:488	arg1	hydrogel					490:497	bacterial cellulose hydrogel	470:497	bacterial cellulose hydrogel (BC)	470:502	We combined bacterial cellulose hydrogel (BC) and nanostructured lipid carriers (NLCs) including doxorubicin (Dox) as a drug model.
29975908	2	28	theme	side	444:447	arg1	effects					449:455	undesirable side effects	432:455	undesirable side effects	432:455	This localized chemotherapy would guarantee high drug concentrations at the tumor site while precluding systemic drug exposure minimizing undesirable side effects.
29975908	5	29	theme	orthotopic	891:900	arg1	model					922:926	an orthotopic breast cancer mouse model	888:926	an orthotopic breast cancer mouse model	888:926	Thereafter, a fixed combination of NLCs-H and NLCs-N loaded into BC (BC-NLCs-NH) was assayed in vivo into an orthotopic breast cancer mouse model.
29975908	1	30	theme	hybrid	104:109	arg1	materials					111:119	hybrid materials	104:119	hybrid materials	104:119	The use of hybrid materials, where a matrix sustains nanoparticles controlling the release of the chemotherapeutic drug, could be beneficial for the treatment of primary tumors prior or after surgery.
29975908	1	31	theme	drug	208:211	arg1	release					176:182	the release	172:182	the release of the chemotherapeutic drug	172:211	The use of hybrid materials, where a matrix sustains nanoparticles controlling the release of the chemotherapeutic drug, could be beneficial for the treatment of primary tumors prior or after surgery.
29975908	0	32	theme	lipid	41:45	arg1	nanoparticles					47:59	lipid nanoparticles	41:59	lipid nanoparticles for localized cancer treatment	41:90	Bacterial cellulose hydrogel loaded with lipid nanoparticles for localized cancer treatment.
29975908	8	33	theme	growth	1427:1432	arg1	reduction					1408:1416	a significant reduction	1394:1416	a significant reduction of tumor growth, metastasis incidence and local drug toxicities	1394:1480	Taking advantage of the differential drug release, a mixture of NLCs-N and NLCs-H was encapsulated into BC matrix (BC-NLCs-NH) and assayed in vivo, showing a significant reduction of tumor growth, metastasis incidence and local drug toxicities.
29975908	6	34	theme	fast	982:985	arg1	release					987:993	fast release	982:993	fast release of the drug	982:1005	NLCs-H showed low encapsulation efficiency (48%) and fast release of the drug while NLCs-N showed higher encapsulation (97%) and sustained drug release.
29975908	5	35	theme	NLCs-H	817:822	arg1	combination					802:812	a fixed combination	794:812	a fixed combination of NLCs-H and NLCs-N loaded into BC (BC-NLCs-NH)	794:861	Thereafter, a fixed combination of NLCs-H and NLCs-N loaded into BC (BC-NLCs-NH) was assayed in vivo into an orthotopic breast cancer mouse model.
29975908	4	36	theme	neutral	632:638	arg1	NLCs-N					645:650	NLCs-N	645:650	NLCs-N	645:650	NLCs loaded with cationic Dox (NLCs-H) or neutral Dox (NLCs-N) were fully characterized and their cell internalization and cytotoxic efficacy were evaluated in vitro against MDA-MB-231 cells.
29975908	4	36	theme	neutral	632:638	arg1	Dox					640:642	neutral Dox	632:642	neutral Dox (NLCs-N)	632:651	NLCs loaded with cationic Dox (NLCs-H) or neutral Dox (NLCs-N) were fully characterized and their cell internalization and cytotoxic efficacy were evaluated in vitro against MDA-MB-231 cells.
29975908	8	37	theme	incidence	1446:1454	arg1	reduction					1408:1416	a significant reduction	1394:1416	a significant reduction of tumor growth, metastasis incidence and local drug toxicities	1394:1480	Taking advantage of the differential drug release, a mixture of NLCs-N and NLCs-H was encapsulated into BC matrix (BC-NLCs-NH) and assayed in vivo, showing a significant reduction of tumor growth, metastasis incidence and local drug toxicities.
29975908	2	38	theme	drug	407:410	arg1	exposure					412:419	systemic drug exposure	398:419	systemic drug exposure	398:419	This localized chemotherapy would guarantee high drug concentrations at the tumor site while precluding systemic drug exposure minimizing undesirable side effects.
29975908	8	39	theme	metastasis	1435:1444	arg1	incidence					1446:1454	metastasis incidence	1435:1454	metastasis incidence	1435:1454	Taking advantage of the differential drug release, a mixture of NLCs-N and NLCs-H was encapsulated into BC matrix (BC-NLCs-NH) and assayed in vivo, showing a significant reduction of tumor growth, metastasis incidence and local drug toxicities.
29975908	8	40	theme	BC	1342:1343	arg1	BC-NLCs-NH					1353:1362	BC-NLCs-NH	1353:1362	BC-NLCs-NH	1353:1362	Taking advantage of the differential drug release, a mixture of NLCs-N and NLCs-H was encapsulated into BC matrix (BC-NLCs-NH) and assayed in vivo, showing a significant reduction of tumor growth, metastasis incidence and local drug toxicities.
29975908	8	40	theme	BC	1342:1343	arg1	matrix					1345:1350	BC matrix	1342:1350	BC matrix (BC-NLCs-NH)	1342:1363	Taking advantage of the differential drug release, a mixture of NLCs-N and NLCs-H was encapsulated into BC matrix (BC-NLCs-NH) and assayed in vivo, showing a significant reduction of tumor growth, metastasis incidence and local drug toxicities.
29975908	1	41	theme	materials	111:119	arg1	use					97:99	The use	93:99	The use	93:99	The use of hybrid materials, where a matrix sustains nanoparticles controlling the release of the chemotherapeutic drug, could be beneficial for the treatment of primary tumors prior or after surgery.
29975908	1	41	theme	materials	111:119	arg1	beneficial					223:232	beneficial	223:232	beneficial	223:232	The use of hybrid materials, where a matrix sustains nanoparticles controlling the release of the chemotherapeutic drug, could be beneficial for the treatment of primary tumors prior or after surgery.
29975908	8	42	theme	local	1460:1464	arg1	toxicities					1471:1480	local drug toxicities	1460:1480	local drug toxicities	1460:1480	Taking advantage of the differential drug release, a mixture of NLCs-N and NLCs-H was encapsulated into BC matrix (BC-NLCs-NH) and assayed in vivo, showing a significant reduction of tumor growth, metastasis incidence and local drug toxicities.
29975908	3	43	theme	drug	578:581	arg1	model					583:587	a drug model	576:587	a drug model	576:587	We combined bacterial cellulose hydrogel (BC) and nanostructured lipid carriers (NLCs) including doxorubicin (Dox) as a drug model.
29975908	3	43	theme	drug	578:581	arg1	carriers					529:536	nanostructured lipid carriers	508:536	nanostructured lipid carriers (NLCs)	508:543	We combined bacterial cellulose hydrogel (BC) and nanostructured lipid carriers (NLCs) including doxorubicin (Dox) as a drug model.
29975908	3	43	theme	drug	578:581	arg1	hydrogel					490:497	bacterial cellulose hydrogel	470:497	bacterial cellulose hydrogel (BC)	470:502	We combined bacterial cellulose hydrogel (BC) and nanostructured lipid carriers (NLCs) including doxorubicin (Dox) as a drug model.
29975908	2	44	theme	high	338:341	arg1	concentrations					348:361	high drug concentrations	338:361	high drug concentrations	338:361	This localized chemotherapy would guarantee high drug concentrations at the tumor site while precluding systemic drug exposure minimizing undesirable side effects.
29975908	8	45	theme	NLCs-H	1313:1318	arg1	mixture					1291:1297	a mixture	1289:1297	a mixture of NLCs-N and NLCs-H	1289:1318	Taking advantage of the differential drug release, a mixture of NLCs-N and NLCs-H was encapsulated into BC matrix (BC-NLCs-NH) and assayed in vivo, showing a significant reduction of tumor growth, metastasis incidence and local drug toxicities.
29975908	4	46	theme	MDA-MB-231	764:773	arg1	cells					775:779	MDA-MB-231 cells	764:779	MDA-MB-231 cells	764:779	NLCs loaded with cationic Dox (NLCs-H) or neutral Dox (NLCs-N) were fully characterized and their cell internalization and cytotoxic efficacy were evaluated in vitro against MDA-MB-231 cells.
29975908	4	47	theme	cell	688:691	arg1	internalization					693:707	their cell internalization	682:707	their cell internalization	682:707	NLCs loaded with cationic Dox (NLCs-H) or neutral Dox (NLCs-N) were fully characterized and their cell internalization and cytotoxic efficacy were evaluated in vitro against MDA-MB-231 cells.
29975908	6	48	theme	drug	1068:1071	arg1	release					1073:1079	sustained drug release	1058:1079	sustained drug release	1058:1079	NLCs-H showed low encapsulation efficiency (48%) and fast release of the drug while NLCs-N showed higher encapsulation (97%) and sustained drug release.
29975908	6	49	theme	low	943:945	arg1	efficiency					961:970	low encapsulation efficiency	943:970	low encapsulation efficiency (48%)	943:976	NLCs-H showed low encapsulation efficiency (48%) and fast release of the drug while NLCs-N showed higher encapsulation (97%) and sustained drug release.
29975908	6	49	theme	low	943:945	arg1	%					975:975	48%	973:975	48%	973:975	NLCs-H showed low encapsulation efficiency (48%) and fast release of the drug while NLCs-N showed higher encapsulation (97%) and sustained drug release.
29975908	8	50	theme	NLCs-N	1302:1307	arg1	mixture					1291:1297	a mixture	1289:1297	a mixture of NLCs-N and NLCs-H	1289:1318	Taking advantage of the differential drug release, a mixture of NLCs-N and NLCs-H was encapsulated into BC matrix (BC-NLCs-NH) and assayed in vivo, showing a significant reduction of tumor growth, metastasis incidence and local drug toxicities.
29975908	8	51	theme	drug	1466:1469	arg1	toxicities					1471:1480	local drug toxicities	1460:1480	local drug toxicities	1460:1480	Taking advantage of the differential drug release, a mixture of NLCs-N and NLCs-H was encapsulated into BC matrix (BC-NLCs-NH) and assayed in vivo, showing a significant reduction of tumor growth, metastasis incidence and local drug toxicities.
29975908	0	52	theme	cancer	75:80	arg1	treatment					82:90	localized cancer treatment	65:90	localized cancer treatment	65:90	Bacterial cellulose hydrogel loaded with lipid nanoparticles for localized cancer treatment.
29975908	1	53	theme	chemotherapeutic	191:206	arg1	drug					208:211	the chemotherapeutic drug	187:211	the chemotherapeutic drug	187:211	The use of hybrid materials, where a matrix sustains nanoparticles controlling the release of the chemotherapeutic drug, could be beneficial for the treatment of primary tumors prior or after surgery.
29975908	2	54	theme	undesirable	432:442	arg1	effects					449:455	undesirable side effects	432:455	undesirable side effects	432:455	This localized chemotherapy would guarantee high drug concentrations at the tumor site while precluding systemic drug exposure minimizing undesirable side effects.
29975908	2	55	theme	tumor	370:374	arg1	site					376:379	the tumor site	366:379	the tumor site	366:379	This localized chemotherapy would guarantee high drug concentrations at the tumor site while precluding systemic drug exposure minimizing undesirable side effects.
29975908	8	56	theme	release	1280:1286	arg1	advantage					1245:1253	advantage	1245:1253	advantage of the differential drug release	1245:1286	Taking advantage of the differential drug release, a mixture of NLCs-N and NLCs-H was encapsulated into BC matrix (BC-NLCs-NH) and assayed in vivo, showing a significant reduction of tumor growth, metastasis incidence and local drug toxicities.
29975908	4	57	theme	cationic	607:614	arg1	NLCs-H					621:626	NLCs-H	621:626	NLCs-H	621:626	NLCs loaded with cationic Dox (NLCs-H) or neutral Dox (NLCs-N) were fully characterized and their cell internalization and cytotoxic efficacy were evaluated in vitro against MDA-MB-231 cells.
29975908	4	57	theme	cationic	607:614	arg1	Dox					616:618	cationic Dox	607:618	cationic Dox (NLCs-H)	607:627	NLCs loaded with cationic Dox (NLCs-H) or neutral Dox (NLCs-N) were fully characterized and their cell internalization and cytotoxic efficacy were evaluated in vitro against MDA-MB-231 cells.
29975908	0	58	theme	localized	65:73	arg1	treatment					82:90	localized cancer treatment	65:90	localized cancer treatment	65:90	Bacterial cellulose hydrogel loaded with lipid nanoparticles for localized cancer treatment.
29975908	6	59	theme	sustained	1058:1066	arg1	release					1073:1079	sustained drug release	1058:1079	sustained drug release	1058:1079	NLCs-H showed low encapsulation efficiency (48%) and fast release of the drug while NLCs-N showed higher encapsulation (97%) and sustained drug release.
29975908	5	60	theme	fixed	796:800	arg1	combination					802:812	a fixed combination	794:812	a fixed combination of NLCs-H and NLCs-N loaded into BC (BC-NLCs-NH)	794:861	Thereafter, a fixed combination of NLCs-H and NLCs-N loaded into BC (BC-NLCs-NH) was assayed in vivo into an orthotopic breast cancer mouse model.
29975908	8	61	theme	toxicities	1471:1480	arg1	reduction					1408:1416	a significant reduction	1394:1416	a significant reduction of tumor growth, metastasis incidence and local drug toxicities	1394:1480	Taking advantage of the differential drug release, a mixture of NLCs-N and NLCs-H was encapsulated into BC matrix (BC-NLCs-NH) and assayed in vivo, showing a significant reduction of tumor growth, metastasis incidence and local drug toxicities.
31872113	6	0	with	units	1295:1299	arg1	ratio					1312:1316	a S/G ratio	1306:1316	a S/G ratio of respectively 1.1 and 2.3	1306:1344	Analytical pyrolysis showed that lignification is a heterogeneous process and the lignin monomeric composition depends on tissue and cell type: cork lignin has a H:G:S ratio of 1:2.5:0.3 and S/G ratio of 0.12, while phloem and wood lignins have mainly G and S units with a S/G ratio of respectively 1.1 and 2.3.
31872113	6	1	theme	G	1287:1287	arg1	units					1295:1299	G and S units	1287:1299	units	1295:1299	Analytical pyrolysis showed that lignification is a heterogeneous process and the lignin monomeric composition depends on tissue and cell type: cork lignin has a H:G:S ratio of 1:2.5:0.3 and S/G ratio of 0.12, while phloem and wood lignins have mainly G and S units with a S/G ratio of respectively 1.1 and 2.3.
31872113	1	2	theme	genetic	244:250	arg1	resources					252:260	potentially differing genetic resources	222:260	potentially differing genetic resources	222:260	Sustainability of cork oak (Quercus suber) forests is threatened by biotic and abiotic factors and characterization of potentially differing genetic resources has therefore gained importance.
31872113	8	3	theme	non-significant	1690:1704	arg1	factor					1706:1711	a non-significant factor	1688:1711	a non-significant factor of chemical variation	1688:1733	NIR spectroscopy and principal component analysis differentiated cork, phloem and wood, while the dispersion within each group highlighted the significant tree variability, while provenances were a non-significant factor of chemical variation.
31872113	8	3	theme	non-significant	1690:1704	arg1	provenances					1671:1681	provenances	1671:1681	provenances	1671:1681	NIR spectroscopy and principal component analysis differentiated cork, phloem and wood, while the dispersion within each group highlighted the significant tree variability, while provenances were a non-significant factor of chemical variation.
31872113	8	4	theme	component	1523:1531	arg1	analysis					1533:1540	principal component analysis	1513:1540	principal component analysis	1513:1540	NIR spectroscopy and principal component analysis differentiated cork, phloem and wood, while the dispersion within each group highlighted the significant tree variability, while provenances were a non-significant factor of chemical variation.
31872113	2	5	link	derived	500:506	arg1	differences					508:518	physiological derived differences	486:518	physiological derived differences	486:518	This work addresses the chemical variation of the three tissues of cork oak stems - cork, phloem and wood - in relation to tree and provenance, looking for genetic chemical diversity and for physiological derived differences.
31872113	5	6	dep	polysaccharides	925:939	arg1	%					946:946	49.1%	942:946	49.1%	942:946	Phloem is more lignified than wood (38.0% vs. 23.4%) and has less polysaccharides (49.1% vs. 64.6%) with glucose-to-other sugars relation of 1:1.3 in phloem and 1:0.7 in wood.
31872113	5	6	dep	polysaccharides	925:939	arg1	%					956:956	64.6%	952:956	64.6%	952:956	Phloem is more lignified than wood (38.0% vs. 23.4%) and has less polysaccharides (49.1% vs. 64.6%) with glucose-to-other sugars relation of 1:1.3 in phloem and 1:0.7 in wood.
31872113	6	7	theme	2.3	1342:1344	arg1	ratio					1312:1316	a S/G ratio	1306:1316	a S/G ratio of respectively 1.1 and 2.3	1306:1344	Analytical pyrolysis showed that lignification is a heterogeneous process and the lignin monomeric composition depends on tissue and cell type: cork lignin has a H:G:S ratio of 1:2.5:0.3 and S/G ratio of 0.12, while phloem and wood lignins have mainly G and S units with a S/G ratio of respectively 1.1 and 2.3.
31872113	6	8	theme	Analytical	1035:1044	arg1	pyrolysis					1046:1054	Analytical pyrolysis	1035:1054	Analytical pyrolysis	1035:1054	Analytical pyrolysis showed that lignification is a heterogeneous process and the lignin monomeric composition depends on tissue and cell type: cork lignin has a H:G:S ratio of 1:2.5:0.3 and S/G ratio of 0.12, while phloem and wood lignins have mainly G and S units with a S/G ratio of respectively 1.1 and 2.3.
31872113	8	9	theme	variation	1725:1733	arg1	factor					1706:1711	a non-significant factor	1688:1711	a non-significant factor of chemical variation	1688:1733	NIR spectroscopy and principal component analysis differentiated cork, phloem and wood, while the dispersion within each group highlighted the significant tree variability, while provenances were a non-significant factor of chemical variation.
31872113	8	9	theme	variation	1725:1733	arg1	provenances					1671:1681	provenances	1671:1681	provenances	1671:1681	NIR spectroscopy and principal component analysis differentiated cork, phloem and wood, while the dispersion within each group highlighted the significant tree variability, while provenances were a non-significant factor of chemical variation.
31872113	6	10	theme	1.1	1334:1336	arg1	ratio					1312:1316	a S/G ratio	1306:1316	a S/G ratio of respectively 1.1 and 2.3	1306:1344	Analytical pyrolysis showed that lignification is a heterogeneous process and the lignin monomeric composition depends on tissue and cell type: cork lignin has a H:G:S ratio of 1:2.5:0.3 and S/G ratio of 0.12, while phloem and wood lignins have mainly G and S units with a S/G ratio of respectively 1.1 and 2.3.
31872113	6	11	theme	monomeric	1124:1132	arg1	composition					1134:1144	the lignin monomeric composition	1113:1144	the lignin monomeric composition	1113:1144	Analytical pyrolysis showed that lignification is a heterogeneous process and the lignin monomeric composition depends on tissue and cell type: cork lignin has a H:G:S ratio of 1:2.5:0.3 and S/G ratio of 0.12, while phloem and wood lignins have mainly G and S units with a S/G ratio of respectively 1.1 and 2.3.
31872113	6	12	theme	wood	1262:1265	arg1	lignins					1267:1273	wood lignins	1262:1273	wood lignins	1262:1273	Analytical pyrolysis showed that lignification is a heterogeneous process and the lignin monomeric composition depends on tissue and cell type: cork lignin has a H:G:S ratio of 1:2.5:0.3 and S/G ratio of 0.12, while phloem and wood lignins have mainly G and S units with a S/G ratio of respectively 1.1 and 2.3.
31872113	7	13	theme	chemical	1425:1432	arg1	variation					1434:1442	some chemical variation	1420:1442	some chemical variation	1420:1442	No significant differences were found between the three provenances, but some chemical variation occurred between the trees within a provenance.
31872113	4	14	theme	lipophilic	837:846	arg1	compounds					848:856	lipophilic compounds	837:856	lipophilic compounds	837:856	Cork is the only tissue where suberin is present (42.3% o.d. mass) as the main cell wall component, and it has a high content of extractives (11.7%) with significant proportion of lipophilic compounds.
31872113	0	15	from	trees	96:100	arg1	phloem					35:40	phloem	35:40	phloem	35:40	Chemical characterization of cork, phloem and wood from different Quercus suber provenances and trees.
31872113	0	15	from	trees	96:100	arg1	cork					29:32	cork	29:32	cork	29:32	Chemical characterization of cork, phloem and wood from different Quercus suber provenances and trees.
31872113	0	15	from	trees	96:100	arg1	characterization					9:24	Chemical characterization	0:24	Chemical characterization of cork, phloem and wood from different Quercus suber provenances and trees.	0:101	Chemical characterization of cork, phloem and wood from different Quercus suber provenances and trees.
31872113	0	15	from	trees	96:100	arg1	wood					46:49	wood	46:49	wood	46:49	Chemical characterization of cork, phloem and wood from different Quercus suber provenances and trees.
31872113	4	16	theme	42.3	707:710	arg1	%					711:711	%	711:711	%	711:711	Cork is the only tissue where suberin is present (42.3% o.d. mass) as the main cell wall component, and it has a high content of extractives (11.7%) with significant proportion of lipophilic compounds.
31872113	6	17	theme	cork	1179:1182	arg1	lignin					1184:1189	cork lignin	1179:1189	cork lignin	1179:1189	Analytical pyrolysis showed that lignification is a heterogeneous process and the lignin monomeric composition depends on tissue and cell type: cork lignin has a H:G:S ratio of 1:2.5:0.3 and S/G ratio of 0.12, while phloem and wood lignins have mainly G and S units with a S/G ratio of respectively 1.1 and 2.3.
31872113	3	18	theme	component	613:621	arg1	ratios					623:628	component ratios	613:628	component ratios	613:628	The three tissues differ with cork clearly differentiating regarding summative composition, component ratios and monomeric composition.
31872113	1	19	theme	oak	126:128	arg1	forests					146:152	cork oak (Quercus suber) forests	121:152	cork oak (Quercus suber) forests	121:152	Sustainability of cork oak (Quercus suber) forests is threatened by biotic and abiotic factors and characterization of potentially differing genetic resources has therefore gained importance.
31872113	2	20	theme	chemical	459:466	arg1	diversity					468:476	genetic chemical diversity	451:476	genetic chemical diversity	451:476	This work addresses the chemical variation of the three tissues of cork oak stems - cork, phloem and wood - in relation to tree and provenance, looking for genetic chemical diversity and for physiological derived differences.
31872113	0	21	from	provenances	80:90	arg1	phloem					35:40	phloem	35:40	phloem	35:40	Chemical characterization of cork, phloem and wood from different Quercus suber provenances and trees.
31872113	0	21	from	provenances	80:90	arg1	cork					29:32	cork	29:32	cork	29:32	Chemical characterization of cork, phloem and wood from different Quercus suber provenances and trees.
31872113	0	21	from	provenances	80:90	arg1	characterization					9:24	Chemical characterization	0:24	Chemical characterization of cork, phloem and wood from different Quercus suber provenances and trees.	0:101	Chemical characterization of cork, phloem and wood from different Quercus suber provenances and trees.
31872113	0	21	from	provenances	80:90	arg1	wood					46:49	wood	46:49	wood	46:49	Chemical characterization of cork, phloem and wood from different Quercus suber provenances and trees.
31872113	6	22	theme	ratio	1230:1234	arg1	ratio					1203:1207	S ratio	1201:1207	a H:G:S ratio of 1:2.5:0.3 and S/G ratio of 0.12	1195:1242	Analytical pyrolysis showed that lignification is a heterogeneous process and the lignin monomeric composition depends on tissue and cell type: cork lignin has a H:G:S ratio of 1:2.5:0.3 and S/G ratio of 0.12, while phloem and wood lignins have mainly G and S units with a S/G ratio of respectively 1.1 and 2.3.
31872113	1	23	theme	Quercus	131:137	arg1	forests					146:152	cork oak (Quercus suber) forests	121:152	cork oak (Quercus suber) forests	121:152	Sustainability of cork oak (Quercus suber) forests is threatened by biotic and abiotic factors and characterization of potentially differing genetic resources has therefore gained importance.
31872113	0	24	theme	Quercus	66:72	arg1	provenances					80:90	different Quercus suber provenances	56:90	different Quercus suber provenances	56:90	Chemical characterization of cork, phloem and wood from different Quercus suber provenances and trees.
31872113	6	25	contain	has	1191:1193	arg1	lignin					1184:1189	cork lignin	1179:1189	cork lignin	1179:1189	Analytical pyrolysis showed that lignification is a heterogeneous process and the lignin monomeric composition depends on tissue and cell type: cork lignin has a H:G:S ratio of 1:2.5:0.3 and S/G ratio of 0.12, while phloem and wood lignins have mainly G and S units with a S/G ratio of respectively 1.1 and 2.3.
31872113	6	25	contain	has	1191:1193	arg2	H					1197:1197	a H	1195:1197	a H:G:S ratio of 1:2.5:0.3 and S/G ratio of 0.12	1195:1242	Analytical pyrolysis showed that lignification is a heterogeneous process and the lignin monomeric composition depends on tissue and cell type: cork lignin has a H:G:S ratio of 1:2.5:0.3 and S/G ratio of 0.12, while phloem and wood lignins have mainly G and S units with a S/G ratio of respectively 1.1 and 2.3.
31872113	8	26	theme	tree	1647:1650	arg1	variability					1652:1662	the significant tree variability	1631:1662	the significant tree variability	1631:1662	NIR spectroscopy and principal component analysis differentiated cork, phloem and wood, while the dispersion within each group highlighted the significant tree variability, while provenances were a non-significant factor of chemical variation.
31872113	4	27	theme	cell	736:739	arg1	component					746:754	the main cell wall component	727:754	the main cell wall component	727:754	Cork is the only tissue where suberin is present (42.3% o.d. mass) as the main cell wall component, and it has a high content of extractives (11.7%) with significant proportion of lipophilic compounds.
31872113	6	28	theme	S	1201:1201	arg1	ratio					1203:1207	S ratio	1201:1207	a H:G:S ratio of 1:2.5:0.3 and S/G ratio of 0.12	1195:1242	Analytical pyrolysis showed that lignification is a heterogeneous process and the lignin monomeric composition depends on tissue and cell type: cork lignin has a H:G:S ratio of 1:2.5:0.3 and S/G ratio of 0.12, while phloem and wood lignins have mainly G and S units with a S/G ratio of respectively 1.1 and 2.3.
31872113	4	29	theme	o.d.	713:716	arg1	mass					718:721	42.3% o.d. mass	707:721	42.3% o.d. mass	707:721	Cork is the only tissue where suberin is present (42.3% o.d. mass) as the main cell wall component, and it has a high content of extractives (11.7%) with significant proportion of lipophilic compounds.
31872113	2	30	theme	stems	371:375	arg1	tissues					351:357	the three tissues	341:357	the three tissues of cork oak stems - cork, phloem and wood -	341:401	This work addresses the chemical variation of the three tissues of cork oak stems - cork, phloem and wood - in relation to tree and provenance, looking for genetic chemical diversity and for physiological derived differences.
31872113	0	31	theme	Chemical	0:7	arg1	characterization					9:24	Chemical characterization	0:24	Chemical characterization of cork, phloem and wood from different Quercus suber provenances and trees.	0:101	Chemical characterization of cork, phloem and wood from different Quercus suber provenances and trees.
31872113	5	32	from	1:0.7	1020:1024	arg1	wood					1029:1032	wood	1029:1032	wood	1029:1032	Phloem is more lignified than wood (38.0% vs. 23.4%) and has less polysaccharides (49.1% vs. 64.6%) with glucose-to-other sugars relation of 1:1.3 in phloem and 1:0.7 in wood.
31872113	5	33	theme	less	920:923	arg1	relation					988:995	less polysaccharides (49.1% vs. 64.6%) with glucose-to-other sugars relation	920:995	less polysaccharides (49.1% vs. 64.6%) with glucose-to-other sugars relation of 1:1.3	920:1004	Phloem is more lignified than wood (38.0% vs. 23.4%) and has less polysaccharides (49.1% vs. 64.6%) with glucose-to-other sugars relation of 1:1.3 in phloem and 1:0.7 in wood.
31872113	4	34	theme	high	770:773	arg1	content					775:781	a high content	768:781	a high content of extractives (11.7%)	768:804	Cork is the only tissue where suberin is present (42.3% o.d. mass) as the main cell wall component, and it has a high content of extractives (11.7%) with significant proportion of lipophilic compounds.
31872113	6	35	theme	0.12	1239:1242	arg1	1:2.5:0.3					1212:1220	1:2.5:0.3	1212:1220	1:2.5:0.3	1212:1220	Analytical pyrolysis showed that lignification is a heterogeneous process and the lignin monomeric composition depends on tissue and cell type: cork lignin has a H:G:S ratio of 1:2.5:0.3 and S/G ratio of 0.12, while phloem and wood lignins have mainly G and S units with a S/G ratio of respectively 1.1 and 2.3.
31872113	6	35	theme	0.12	1239:1242	arg1	ratio					1230:1234	S/G ratio	1226:1234	S/G ratio	1226:1234	Analytical pyrolysis showed that lignification is a heterogeneous process and the lignin monomeric composition depends on tissue and cell type: cork lignin has a H:G:S ratio of 1:2.5:0.3 and S/G ratio of 0.12, while phloem and wood lignins have mainly G and S units with a S/G ratio of respectively 1.1 and 2.3.
31872113	2	36	dep	stems	371:375	arg1	phloem					385:390	phloem	385:390	phloem	385:390	This work addresses the chemical variation of the three tissues of cork oak stems - cork, phloem and wood - in relation to tree and provenance, looking for genetic chemical diversity and for physiological derived differences.
31872113	2	36	dep	stems	371:375	arg1	cork					379:382	cork	379:382	cork	379:382	This work addresses the chemical variation of the three tissues of cork oak stems - cork, phloem and wood - in relation to tree and provenance, looking for genetic chemical diversity and for physiological derived differences.
31872113	2	36	dep	stems	371:375	arg1	stems					371:375	cork oak stems	362:375	cork oak stems - cork, phloem and wood -	362:401	This work addresses the chemical variation of the three tissues of cork oak stems - cork, phloem and wood - in relation to tree and provenance, looking for genetic chemical diversity and for physiological derived differences.
31872113	2	36	dep	stems	371:375	arg1	wood					396:399	wood	396:399	wood	396:399	This work addresses the chemical variation of the three tissues of cork oak stems - cork, phloem and wood - in relation to tree and provenance, looking for genetic chemical diversity and for physiological derived differences.
31872113	4	37	theme	only	669:672	arg1	Cork					657:660	Cork	657:660	Cork	657:660	Cork is the only tissue where suberin is present (42.3% o.d. mass) as the main cell wall component, and it has a high content of extractives (11.7%) with significant proportion of lipophilic compounds.
31872113	4	37	theme	only	669:672	arg1	tissue					674:679	the only tissue	665:679	the only tissue where suberin is present (42.3% o.d. mass) as the main cell wall component	665:754	Cork is the only tissue where suberin is present (42.3% o.d. mass) as the main cell wall component, and it has a high content of extractives (11.7%) with significant proportion of lipophilic compounds.
31872113	2	38	theme	cork	362:365	arg1	phloem					385:390	phloem	385:390	phloem	385:390	This work addresses the chemical variation of the three tissues of cork oak stems - cork, phloem and wood - in relation to tree and provenance, looking for genetic chemical diversity and for physiological derived differences.
31872113	2	38	theme	cork	362:365	arg1	cork					379:382	cork	379:382	cork	379:382	This work addresses the chemical variation of the three tissues of cork oak stems - cork, phloem and wood - in relation to tree and provenance, looking for genetic chemical diversity and for physiological derived differences.
31872113	2	38	theme	cork	362:365	arg1	stems					371:375	cork oak stems	362:375	cork oak stems - cork, phloem and wood -	362:401	This work addresses the chemical variation of the three tissues of cork oak stems - cork, phloem and wood - in relation to tree and provenance, looking for genetic chemical diversity and for physiological derived differences.
31872113	2	38	theme	cork	362:365	arg1	wood					396:399	wood	396:399	wood	396:399	This work addresses the chemical variation of the three tissues of cork oak stems - cork, phloem and wood - in relation to tree and provenance, looking for genetic chemical diversity and for physiological derived differences.
31872113	6	39	theme	S/G	1226:1228	arg1	ratio					1230:1234	S/G ratio	1226:1234	S/G ratio	1226:1234	Analytical pyrolysis showed that lignification is a heterogeneous process and the lignin monomeric composition depends on tissue and cell type: cork lignin has a H:G:S ratio of 1:2.5:0.3 and S/G ratio of 0.12, while phloem and wood lignins have mainly G and S units with a S/G ratio of respectively 1.1 and 2.3.
31872113	2	40	theme	tissues	351:357	arg1	variation					328:336	the chemical variation	315:336	the chemical variation of the three tissues of cork oak stems - cork, phloem and wood - in relation to tree and provenance	315:436	This work addresses the chemical variation of the three tissues of cork oak stems - cork, phloem and wood - in relation to tree and provenance, looking for genetic chemical diversity and for physiological derived differences.
31872113	1	41	theme	differing	234:242	arg1	resources					252:260	potentially differing genetic resources	222:260	potentially differing genetic resources	222:260	Sustainability of cork oak (Quercus suber) forests is threatened by biotic and abiotic factors and characterization of potentially differing genetic resources has therefore gained importance.
31872113	2	42	from	variation	328:336	arg1	relation					406:413	relation	406:413	relation to tree and provenance	406:436	This work addresses the chemical variation of the three tissues of cork oak stems - cork, phloem and wood - in relation to tree and provenance, looking for genetic chemical diversity and for physiological derived differences.
31872113	5	43	with	polysaccharides	925:939	arg1	sugars					981:986	glucose-to-other sugars	964:986	glucose-to-other sugars	964:986	Phloem is more lignified than wood (38.0% vs. 23.4%) and has less polysaccharides (49.1% vs. 64.6%) with glucose-to-other sugars relation of 1:1.3 in phloem and 1:0.7 in wood.
31872113	6	44	theme	cell	1168:1171	arg1	type					1173:1176	cell type	1168:1176	cell type	1168:1176	Analytical pyrolysis showed that lignification is a heterogeneous process and the lignin monomeric composition depends on tissue and cell type: cork lignin has a H:G:S ratio of 1:2.5:0.3 and S/G ratio of 0.12, while phloem and wood lignins have mainly G and S units with a S/G ratio of respectively 1.1 and 2.3.
31872113	1	45	theme	resources	252:260	arg1	characterization					202:217	characterization	202:217	characterization of potentially differing genetic resources	202:260	Sustainability of cork oak (Quercus suber) forests is threatened by biotic and abiotic factors and characterization of potentially differing genetic resources has therefore gained importance.
31872113	2	46	dep	derived	500:506	arg1	physiological					486:498	physiological	486:498	physiological	486:498	This work addresses the chemical variation of the three tissues of cork oak stems - cork, phloem and wood - in relation to tree and provenance, looking for genetic chemical diversity and for physiological derived differences.
31872113	8	47	theme	chemical	1716:1723	arg1	variation					1725:1733	chemical variation	1716:1733	chemical variation	1716:1733	NIR spectroscopy and principal component analysis differentiated cork, phloem and wood, while the dispersion within each group highlighted the significant tree variability, while provenances were a non-significant factor of chemical variation.
31872113	8	48	theme	principal	1513:1521	arg1	analysis					1533:1540	principal component analysis	1513:1540	principal component analysis	1513:1540	NIR spectroscopy and principal component analysis differentiated cork, phloem and wood, while the dispersion within each group highlighted the significant tree variability, while provenances were a non-significant factor of chemical variation.
31872113	1	49	theme	forests	146:152	arg1	Sustainability					103:116	Sustainability	103:116	Sustainability of cork oak (Quercus suber) forests	103:152	Sustainability of cork oak (Quercus suber) forests is threatened by biotic and abiotic factors and characterization of potentially differing genetic resources has therefore gained importance.
31872113	5	50	from	phloem	1009:1014	arg1	wood					1029:1032	wood	1029:1032	wood	1029:1032	Phloem is more lignified than wood (38.0% vs. 23.4%) and has less polysaccharides (49.1% vs. 64.6%) with glucose-to-other sugars relation of 1:1.3 in phloem and 1:0.7 in wood.
31872113	4	51	theme	extractives	786:796	arg1	content					775:781	a high content	768:781	a high content of extractives (11.7%)	768:804	Cork is the only tissue where suberin is present (42.3% o.d. mass) as the main cell wall component, and it has a high content of extractives (11.7%) with significant proportion of lipophilic compounds.
31872113	5	52	dep	less	920:923	arg1	polysaccharides					925:939	polysaccharides	925:939	polysaccharides (49.1% vs. 64.6%) with glucose-to-other sugars	925:986	Phloem is more lignified than wood (38.0% vs. 23.4%) and has less polysaccharides (49.1% vs. 64.6%) with glucose-to-other sugars relation of 1:1.3 in phloem and 1:0.7 in wood.
31872113	1	53	theme	biotic	171:176	arg1	factors					190:196	biotic and abiotic factors	171:196	biotic and abiotic factors	171:196	Sustainability of cork oak (Quercus suber) forests is threatened by biotic and abiotic factors and characterization of potentially differing genetic resources has therefore gained importance.
31872113	4	54	theme	compounds	848:856	arg1	proportion					823:832	significant proportion	811:832	significant proportion of lipophilic compounds	811:856	Cork is the only tissue where suberin is present (42.3% o.d. mass) as the main cell wall component, and it has a high content of extractives (11.7%) with significant proportion of lipophilic compounds.
31872113	1	55	theme	abiotic	182:188	arg1	factors					190:196	biotic and abiotic factors	171:196	biotic and abiotic factors	171:196	Sustainability of cork oak (Quercus suber) forests is threatened by biotic and abiotic factors and characterization of potentially differing genetic resources has therefore gained importance.
31872113	6	56	dep	H	1197:1197	arg1	G					1199:1199	G	1199:1199	a H:G:S ratio of 1:2.5:0.3 and S/G ratio of 0.12	1195:1242	Analytical pyrolysis showed that lignification is a heterogeneous process and the lignin monomeric composition depends on tissue and cell type: cork lignin has a H:G:S ratio of 1:2.5:0.3 and S/G ratio of 0.12, while phloem and wood lignins have mainly G and S units with a S/G ratio of respectively 1.1 and 2.3.
31872113	6	56	dep	H	1197:1197	arg1	ratio					1203:1207	S ratio	1201:1207	a H:G:S ratio of 1:2.5:0.3 and S/G ratio of 0.12	1195:1242	Analytical pyrolysis showed that lignification is a heterogeneous process and the lignin monomeric composition depends on tissue and cell type: cork lignin has a H:G:S ratio of 1:2.5:0.3 and S/G ratio of 0.12, while phloem and wood lignins have mainly G and S units with a S/G ratio of respectively 1.1 and 2.3.
31872113	2	57	theme	derived	500:506	arg1	differences					508:518	physiological derived differences	486:518	physiological derived differences	486:518	This work addresses the chemical variation of the three tissues of cork oak stems - cork, phloem and wood - in relation to tree and provenance, looking for genetic chemical diversity and for physiological derived differences.
31872113	6	58	theme	lignin	1117:1122	arg1	composition					1134:1144	the lignin monomeric composition	1113:1144	the lignin monomeric composition	1113:1144	Analytical pyrolysis showed that lignification is a heterogeneous process and the lignin monomeric composition depends on tissue and cell type: cork lignin has a H:G:S ratio of 1:2.5:0.3 and S/G ratio of 0.12, while phloem and wood lignins have mainly G and S units with a S/G ratio of respectively 1.1 and 2.3.
31872113	5	59	theme	glucose-to-other	964:979	arg1	sugars					981:986	glucose-to-other sugars	964:986	glucose-to-other sugars	964:986	Phloem is more lignified than wood (38.0% vs. 23.4%) and has less polysaccharides (49.1% vs. 64.6%) with glucose-to-other sugars relation of 1:1.3 in phloem and 1:0.7 in wood.
31872113	4	60	theme	significant	811:821	arg1	proportion					823:832	significant proportion	811:832	significant proportion of lipophilic compounds	811:856	Cork is the only tissue where suberin is present (42.3% o.d. mass) as the main cell wall component, and it has a high content of extractives (11.7%) with significant proportion of lipophilic compounds.
31872113	1	61	theme	cork	121:124	arg1	forests					146:152	cork oak (Quercus suber) forests	121:152	cork oak (Quercus suber) forests	121:152	Sustainability of cork oak (Quercus suber) forests is threatened by biotic and abiotic factors and characterization of potentially differing genetic resources has therefore gained importance.
31872113	6	62	theme	heterogeneous	1087:1099	arg1	process					1101:1107	a heterogeneous process	1085:1107	a heterogeneous process	1085:1107	Analytical pyrolysis showed that lignification is a heterogeneous process and the lignin monomeric composition depends on tissue and cell type: cork lignin has a H:G:S ratio of 1:2.5:0.3 and S/G ratio of 0.12, while phloem and wood lignins have mainly G and S units with a S/G ratio of respectively 1.1 and 2.3.
31872113	6	62	theme	heterogeneous	1087:1099	arg1	lignification					1068:1080	lignification	1068:1080	lignification	1068:1080	Analytical pyrolysis showed that lignification is a heterogeneous process and the lignin monomeric composition depends on tissue and cell type: cork lignin has a H:G:S ratio of 1:2.5:0.3 and S/G ratio of 0.12, while phloem and wood lignins have mainly G and S units with a S/G ratio of respectively 1.1 and 2.3.
31872113	0	63	theme	different	56:64	arg1	provenances					80:90	different Quercus suber provenances	56:90	different Quercus suber provenances	56:90	Chemical characterization of cork, phloem and wood from different Quercus suber provenances and trees.
31872113	3	64	theme	summative	590:598	arg1	composition					600:610	summative composition	590:610	summative composition	590:610	The three tissues differ with cork clearly differentiating regarding summative composition, component ratios and monomeric composition.
31872113	2	65	theme	genetic	451:457	arg1	diversity					468:476	genetic chemical diversity	451:476	genetic chemical diversity	451:476	This work addresses the chemical variation of the three tissues of cork oak stems - cork, phloem and wood - in relation to tree and provenance, looking for genetic chemical diversity and for physiological derived differences.
31872113	5	66	dep	wood	889:892	arg1	%					899:899	38.0%	895:899	38.0%	895:899	Phloem is more lignified than wood (38.0% vs. 23.4%) and has less polysaccharides (49.1% vs. 64.6%) with glucose-to-other sugars relation of 1:1.3 in phloem and 1:0.7 in wood.
31872113	5	66	dep	wood	889:892	arg1	%					909:909	23.4%	905:909	23.4%	905:909	Phloem is more lignified than wood (38.0% vs. 23.4%) and has less polysaccharides (49.1% vs. 64.6%) with glucose-to-other sugars relation of 1:1.3 in phloem and 1:0.7 in wood.
31872113	0	67	theme	suber	74:78	arg1	provenances					80:90	different Quercus suber provenances	56:90	different Quercus suber provenances	56:90	Chemical characterization of cork, phloem and wood from different Quercus suber provenances and trees.
31872113	1	68	theme	suber	139:143	arg1	forests					146:152	cork oak (Quercus suber) forests	121:152	cork oak (Quercus suber) forests	121:152	Sustainability of cork oak (Quercus suber) forests is threatened by biotic and abiotic factors and characterization of potentially differing genetic resources has therefore gained importance.
31872113	0	69	from	characterization	9:24	arg1	trees					96:100	trees	96:100	trees	96:100	Chemical characterization of cork, phloem and wood from different Quercus suber provenances and trees.
31872113	0	69	from	characterization	9:24	arg1	provenances					80:90	different Quercus suber provenances	56:90	different Quercus suber provenances	56:90	Chemical characterization of cork, phloem and wood from different Quercus suber provenances and trees.
31872113	6	70	theme	1:2.5:0.3	1212:1220	arg1	ratio					1203:1207	S ratio	1201:1207	a H:G:S ratio of 1:2.5:0.3 and S/G ratio of 0.12	1195:1242	Analytical pyrolysis showed that lignification is a heterogeneous process and the lignin monomeric composition depends on tissue and cell type: cork lignin has a H:G:S ratio of 1:2.5:0.3 and S/G ratio of 0.12, while phloem and wood lignins have mainly G and S units with a S/G ratio of respectively 1.1 and 2.3.
31872113	7	71	theme	significant	1350:1360	arg1	differences					1362:1372	No significant differences	1347:1372	No significant differences	1347:1372	No significant differences were found between the three provenances, but some chemical variation occurred between the trees within a provenance.
31872113	4	72	contain	has	764:766	arg2	content					775:781	a high content	768:781	a high content of extractives (11.7%)	768:804	Cork is the only tissue where suberin is present (42.3% o.d. mass) as the main cell wall component, and it has a high content of extractives (11.7%) with significant proportion of lipophilic compounds.
31872113	4	72	contain	has	764:766	arg1	it					761:762	it	761:762	it	761:762	Cork is the only tissue where suberin is present (42.3% o.d. mass) as the main cell wall component, and it has a high content of extractives (11.7%) with significant proportion of lipophilic compounds.
31872113	8	73	theme	significant	1635:1645	arg1	variability					1652:1662	the significant tree variability	1631:1662	the significant tree variability	1631:1662	NIR spectroscopy and principal component analysis differentiated cork, phloem and wood, while the dispersion within each group highlighted the significant tree variability, while provenances were a non-significant factor of chemical variation.
31872113	4	74	theme	main	731:734	arg1	component					746:754	the main cell wall component	727:754	the main cell wall component	727:754	Cork is the only tissue where suberin is present (42.3% o.d. mass) as the main cell wall component, and it has a high content of extractives (11.7%) with significant proportion of lipophilic compounds.
31872113	5	75	theme	1:1.3	1000:1004	arg1	relation					988:995	less polysaccharides (49.1% vs. 64.6%) with glucose-to-other sugars relation	920:995	less polysaccharides (49.1% vs. 64.6%) with glucose-to-other sugars relation of 1:1.3	920:1004	Phloem is more lignified than wood (38.0% vs. 23.4%) and has less polysaccharides (49.1% vs. 64.6%) with glucose-to-other sugars relation of 1:1.3 in phloem and 1:0.7 in wood.
31872113	4	76	theme	%	711:711	arg1	mass					718:721	42.3% o.d. mass	707:721	42.3% o.d. mass	707:721	Cork is the only tissue where suberin is present (42.3% o.d. mass) as the main cell wall component, and it has a high content of extractives (11.7%) with significant proportion of lipophilic compounds.
31872113	2	77	theme	oak	367:369	arg1	phloem					385:390	phloem	385:390	phloem	385:390	This work addresses the chemical variation of the three tissues of cork oak stems - cork, phloem and wood - in relation to tree and provenance, looking for genetic chemical diversity and for physiological derived differences.
31872113	2	77	theme	oak	367:369	arg1	cork					379:382	cork	379:382	cork	379:382	This work addresses the chemical variation of the three tissues of cork oak stems - cork, phloem and wood - in relation to tree and provenance, looking for genetic chemical diversity and for physiological derived differences.
31872113	2	77	theme	oak	367:369	arg1	stems					371:375	cork oak stems	362:375	cork oak stems - cork, phloem and wood -	362:401	This work addresses the chemical variation of the three tissues of cork oak stems - cork, phloem and wood - in relation to tree and provenance, looking for genetic chemical diversity and for physiological derived differences.
31872113	2	77	theme	oak	367:369	arg1	wood					396:399	wood	396:399	wood	396:399	This work addresses the chemical variation of the three tissues of cork oak stems - cork, phloem and wood - in relation to tree and provenance, looking for genetic chemical diversity and for physiological derived differences.
31872113	0	78	theme	cork	29:32	arg1	characterization					9:24	Chemical characterization	0:24	Chemical characterization of cork, phloem and wood from different Quercus suber provenances and trees.	0:101	Chemical characterization of cork, phloem and wood from different Quercus suber provenances and trees.
31872113	4	79	dep	present	698:704	arg1	mass					718:721	42.3% o.d. mass	707:721	42.3% o.d. mass	707:721	Cork is the only tissue where suberin is present (42.3% o.d. mass) as the main cell wall component, and it has a high content of extractives (11.7%) with significant proportion of lipophilic compounds.
31872113	3	80	theme	monomeric	634:642	arg1	composition					644:654	monomeric composition	634:654	monomeric composition	634:654	The three tissues differ with cork clearly differentiating regarding summative composition, component ratios and monomeric composition.
31872113	0	81	theme	phloem	35:40	arg1	characterization					9:24	Chemical characterization	0:24	Chemical characterization of cork, phloem and wood from different Quercus suber provenances and trees.	0:101	Chemical characterization of cork, phloem and wood from different Quercus suber provenances and trees.
31872113	0	82	theme	wood	46:49	arg1	characterization					9:24	Chemical characterization	0:24	Chemical characterization of cork, phloem and wood from different Quercus suber provenances and trees.	0:101	Chemical characterization of cork, phloem and wood from different Quercus suber provenances and trees.
31872113	6	83	theme	S/G	1308:1310	arg1	ratio					1312:1316	a S/G ratio	1306:1316	a S/G ratio of respectively 1.1 and 2.3	1306:1344	Analytical pyrolysis showed that lignification is a heterogeneous process and the lignin monomeric composition depends on tissue and cell type: cork lignin has a H:G:S ratio of 1:2.5:0.3 and S/G ratio of 0.12, while phloem and wood lignins have mainly G and S units with a S/G ratio of respectively 1.1 and 2.3.
31872113	6	84	contain	have	1275:1278	arg1	phloem					1251:1256	phloem	1251:1256	phloem	1251:1256	Analytical pyrolysis showed that lignification is a heterogeneous process and the lignin monomeric composition depends on tissue and cell type: cork lignin has a H:G:S ratio of 1:2.5:0.3 and S/G ratio of 0.12, while phloem and wood lignins have mainly G and S units with a S/G ratio of respectively 1.1 and 2.3.
31872113	6	84	contain	have	1275:1278	arg1	lignins					1267:1273	wood lignins	1262:1273	wood lignins	1262:1273	Analytical pyrolysis showed that lignification is a heterogeneous process and the lignin monomeric composition depends on tissue and cell type: cork lignin has a H:G:S ratio of 1:2.5:0.3 and S/G ratio of 0.12, while phloem and wood lignins have mainly G and S units with a S/G ratio of respectively 1.1 and 2.3.
31872113	6	84	contain	have	1275:1278	arg2	units					1295:1299	G and S units	1287:1299	units	1295:1299	Analytical pyrolysis showed that lignification is a heterogeneous process and the lignin monomeric composition depends on tissue and cell type: cork lignin has a H:G:S ratio of 1:2.5:0.3 and S/G ratio of 0.12, while phloem and wood lignins have mainly G and S units with a S/G ratio of respectively 1.1 and 2.3.
31872113	4	85	theme	wall	741:744	arg1	component					746:754	the main cell wall component	727:754	the main cell wall component	727:754	Cork is the only tissue where suberin is present (42.3% o.d. mass) as the main cell wall component, and it has a high content of extractives (11.7%) with significant proportion of lipophilic compounds.
31872113	2	86	theme	chemical	319:326	arg1	variation					328:336	the chemical variation	315:336	the chemical variation of the three tissues of cork oak stems - cork, phloem and wood - in relation to tree and provenance	315:436	This work addresses the chemical variation of the three tissues of cork oak stems - cork, phloem and wood - in relation to tree and provenance, looking for genetic chemical diversity and for physiological derived differences.
31872113	6	87	theme	S	1293:1293	arg1	units					1295:1299	G and S units	1287:1299	units	1295:1299	Analytical pyrolysis showed that lignification is a heterogeneous process and the lignin monomeric composition depends on tissue and cell type: cork lignin has a H:G:S ratio of 1:2.5:0.3 and S/G ratio of 0.12, while phloem and wood lignins have mainly G and S units with a S/G ratio of respectively 1.1 and 2.3.
31872113	5	88	contain	has	916:918	arg1	Phloem					859:864	Phloem	859:864	Phloem	859:864	Phloem is more lignified than wood (38.0% vs. 23.4%) and has less polysaccharides (49.1% vs. 64.6%) with glucose-to-other sugars relation of 1:1.3 in phloem and 1:0.7 in wood.
31872113	5	88	contain	has	916:918	arg2	relation					988:995	less polysaccharides (49.1% vs. 64.6%) with glucose-to-other sugars relation	920:995	less polysaccharides (49.1% vs. 64.6%) with glucose-to-other sugars relation of 1:1.3	920:1004	Phloem is more lignified than wood (38.0% vs. 23.4%) and has less polysaccharides (49.1% vs. 64.6%) with glucose-to-other sugars relation of 1:1.3 in phloem and 1:0.7 in wood.
31872113	8	89	theme	NIR	1492:1494	arg1	spectroscopy					1496:1507	NIR spectroscopy	1492:1507	NIR spectroscopy	1492:1507	NIR spectroscopy and principal component analysis differentiated cork, phloem and wood, while the dispersion within each group highlighted the significant tree variability, while provenances were a non-significant factor of chemical variation.
29882877	14	0	theme	cell	2040:2043	arg1	proliferation					2045:2057	cell proliferation	2040:2057	cell proliferation of HUVEC	2040:2066	Both, in the volume phase and immobilized at the surface, PECNP composed of PLL/HEP and PLL/DS clearly reduced cell proliferation of HUVEC, however without an apparent cytotoxic effect, while PLL/CS composition showed minor impairment.
29882877	17	1	theme	growth	2492:2497	arg1	factors					2499:2505	growth factors	2492:2505	growth factors	2492:2505	Since uptake and binding for PLL/HEP was more efficient than for PLL/DS, PECNP of PLL/HEP may be used to deliver growth factors to endothelial cells during vascularization of bone reconstitution material, whereas those of PLL/CS may have an advantage for substituting biomimetic bone scaffold material.
29882877	7	2	theme	umbilical	1122:1130	arg1	HUVEC					1161:1165	HUVEC	1161:1165	HUVEC	1161:1165	PECNP were applied to human umbilical cord vein endothelial cells (HUVEC) in both, the volume phase and immobilized phase at model substrates like tissue culture dishes.
29882877	7	2	theme	umbilical	1122:1130	arg1	cells					1154:1158	human umbilical cord vein endothelial cells	1116:1158	human umbilical cord vein endothelial cells (HUVEC) in both, the volume phase and immobilized phase at model substrates like tissue culture dishes	1116:1261	PECNP were applied to human umbilical cord vein endothelial cells (HUVEC) in both, the volume phase and immobilized phase at model substrates like tissue culture dishes.
29882877	2	3	theme	encounter	305:313	arg1	EC					334:335	EC	334:335	EC	334:335	If nanoparticles (NP) are used as vectors for drug delivery, they have to encounter endothelial cells (EC) lining the vascular lumen, if applied intravenously.
29882877	2	3	theme	encounter	305:313	arg1	cells					327:331	encounter endothelial cells	305:331	encounter endothelial cells (EC) lining the vascular lumen, if applied intravenously	305:388	If nanoparticles (NP) are used as vectors for drug delivery, they have to encounter endothelial cells (EC) lining the vascular lumen, if applied intravenously.
29882877	11	4	from	effects	1664:1670	arg1	uptake					1710:1715	uptake	1710:1715	uptake	1710:1715	PECNP in the volume phase showed no effect of the net charge sign and only minor effects of the composition on the binding and uptake of PECNP at HUVEC.
29882877	11	4	from	effects	1664:1670	arg1	binding					1698:1704	binding	1698:1704	binding	1698:1704	PECNP in the volume phase showed no effect of the net charge sign and only minor effects of the composition on the binding and uptake of PECNP at HUVEC.
29882877	16	5	with	obvious	2342:2348	arg1	PLL/CS					2371:2376	the composition PLL/CS	2355:2376	the composition PLL/CS	2355:2376	The negative effects were less obvious with the composition PLL/CS.
29882877	6	6	theme	negative	1059:1066	arg1	charge					1072:1077	positive or negative net charge	1047:1077	positive or negative net charge	1047:1077	PECNP, composed of PLL in combination with the polysaccharides dextran sulfate (DS), cellulose sulfate (CS) or heparin (HEP), either unlabeled or labeled with fluorescein isothiocyanate (FITC) and either with positive or negative net charge were prepared.
29882877	10	7	theme	small	1506:1510	arg1	percentage					1512:1521	A small percentage	1504:1521	A small percentage of immobilized PECNP	1504:1542	A small percentage of immobilized PECNP was taken up by cells during adhesion.
29882877	17	8	dep	used	2476:2479	arg1	whereas					2584:2590	whereas	2584:2590	whereas	2584:2590	Since uptake and binding for PLL/HEP was more efficient than for PLL/DS, PECNP of PLL/HEP may be used to deliver growth factors to endothelial cells during vascularization of bone reconstitution material, whereas those of PLL/CS may have an advantage for substituting biomimetic bone scaffold material.
29882877	1	9	theme	tissue	165:170	arg1	regeneration					172:183	tissue regeneration	165:183	tissue regeneration	165:183	Angiogenesis plays an important role in both soft and hard tissue regeneration, which can be modulated by therapeutic drugs.
29882877	6	10	with	combination	864:874	arg1	heparin					949:955	heparin	949:955	heparin (HEP)	949:961	PECNP, composed of PLL in combination with the polysaccharides dextran sulfate (DS), cellulose sulfate (CS) or heparin (HEP), either unlabeled or labeled with fluorescein isothiocyanate (FITC) and either with positive or negative net charge were prepared.
29882877	6	10	with	combination	864:874	arg1	HEP					958:960	HEP	958:960	HEP	958:960	PECNP, composed of PLL in combination with the polysaccharides dextran sulfate (DS), cellulose sulfate (CS) or heparin (HEP), either unlabeled or labeled with fluorescein isothiocyanate (FITC) and either with positive or negative net charge were prepared.
29882877	6	10	with	combination	864:874	arg1	sulfate					909:915	the polysaccharides dextran sulfate	881:915	the polysaccharides dextran sulfate (DS)	881:920	PECNP, composed of PLL in combination with the polysaccharides dextran sulfate (DS), cellulose sulfate (CS) or heparin (HEP), either unlabeled or labeled with fluorescein isothiocyanate (FITC) and either with positive or negative net charge were prepared.
29882877	6	10	with	combination	864:874	arg1	sulfate					933:939	cellulose sulfate	923:939	cellulose sulfate (CS)	923:944	PECNP, composed of PLL in combination with the polysaccharides dextran sulfate (DS), cellulose sulfate (CS) or heparin (HEP), either unlabeled or labeled with fluorescein isothiocyanate (FITC) and either with positive or negative net charge were prepared.
29882877	6	10	with	combination	864:874	arg1	CS					942:943	CS	942:943	CS	942:943	PECNP, composed of PLL in combination with the polysaccharides dextran sulfate (DS), cellulose sulfate (CS) or heparin (HEP), either unlabeled or labeled with fluorescein isothiocyanate (FITC) and either with positive or negative net charge were prepared.
29882877	6	10	with	combination	864:874	arg1	DS					918:919	DS	918:919	DS	918:919	PECNP, composed of PLL in combination with the polysaccharides dextran sulfate (DS), cellulose sulfate (CS) or heparin (HEP), either unlabeled or labeled with fluorescein isothiocyanate (FITC) and either with positive or negative net charge were prepared.
29882877	11	11	theme	sign	1644:1647	arg1	effect					1619:1624	no effect	1616:1624	no effect of the net charge sign	1616:1647	PECNP in the volume phase showed no effect of the net charge sign and only minor effects of the composition on the binding and uptake of PECNP at HUVEC.
29882877	11	11	theme	sign	1644:1647	arg1	effects					1664:1670	only minor effects	1653:1670	only minor effects of the composition on the binding and uptake of PECNP at HUVEC	1653:1733	PECNP in the volume phase showed no effect of the net charge sign and only minor effects of the composition on the binding and uptake of PECNP at HUVEC.
29882877	2	12	theme	drug	277:280	arg1	delivery					282:289	drug delivery	277:289	drug delivery	277:289	If nanoparticles (NP) are used as vectors for drug delivery, they have to encounter endothelial cells (EC) lining the vascular lumen, if applied intravenously.
29882877	12	13	theme	apparent	1807:1814	arg1	processing					1824:1833	apparent further processing	1807:1833	apparent further processing	1807:1833	PECNP were stored in endosomal vesicles in a cumulative manner without apparent further processing.
29882877	11	14	theme	net	1633:1635	arg1	charge					1637:1642	the net charge	1629:1642	the net charge sign	1629:1647	PECNP in the volume phase showed no effect of the net charge sign and only minor effects of the composition on the binding and uptake of PECNP at HUVEC.
29882877	6	15	theme	polysaccharides	885:899	arg1	DS					918:919	DS	918:919	DS	918:919	PECNP, composed of PLL in combination with the polysaccharides dextran sulfate (DS), cellulose sulfate (CS) or heparin (HEP), either unlabeled or labeled with fluorescein isothiocyanate (FITC) and either with positive or negative net charge were prepared.
29882877	6	15	theme	polysaccharides	885:899	arg1	sulfate					909:915	the polysaccharides dextran sulfate	881:915	the polysaccharides dextran sulfate (DS)	881:920	PECNP, composed of PLL in combination with the polysaccharides dextran sulfate (DS), cellulose sulfate (CS) or heparin (HEP), either unlabeled or labeled with fluorescein isothiocyanate (FITC) and either with positive or negative net charge were prepared.
29882877	17	16	theme	PLL/HEP	2461:2467	arg1	PECNP					2452:2456	PECNP	2452:2456	PECNP of PLL/HEP	2452:2467	Since uptake and binding for PLL/HEP was more efficient than for PLL/DS, PECNP of PLL/HEP may be used to deliver growth factors to endothelial cells during vascularization of bone reconstitution material, whereas those of PLL/CS may have an advantage for substituting biomimetic bone scaffold material.
29882877	7	17	theme	culture	1248:1254	arg1	dishes					1256:1261	tissue culture dishes	1241:1261	tissue culture dishes	1241:1261	PECNP were applied to human umbilical cord vein endothelial cells (HUVEC) in both, the volume phase and immobilized phase at model substrates like tissue culture dishes.
29882877	15	18	theme	HUVEC	2304:2308	arg1	rate					2296:2299	the proliferation rate	2278:2299	the proliferation rate of HUVEC	2278:2308	PECNP have an anti-adhesive effect on HUVEC and are taken up by endothelial cells which may negatively influence the proliferation rate of HUVEC.
29882877	6	19	theme	fluorescein	997:1007	arg1	FITC					1025:1028	FITC	1025:1028	FITC	1025:1028	PECNP, composed of PLL in combination with the polysaccharides dextran sulfate (DS), cellulose sulfate (CS) or heparin (HEP), either unlabeled or labeled with fluorescein isothiocyanate (FITC) and either with positive or negative net charge were prepared.
29882877	6	19	theme	fluorescein	997:1007	arg1	isothiocyanate					1009:1022	fluorescein isothiocyanate	997:1022	fluorescein isothiocyanate (FITC)	997:1029	PECNP, composed of PLL in combination with the polysaccharides dextran sulfate (DS), cellulose sulfate (CS) or heparin (HEP), either unlabeled or labeled with fluorescein isothiocyanate (FITC) and either with positive or negative net charge were prepared.
29882877	3	20	theme	various	518:524	arg1	polysaccharides					534:548	various anionic polysaccharides	518:548	various anionic polysaccharides with human vascular endothelial cells (HUVEC)	518:594	Herein the interaction of unloaded polyelectrolyte complex nanoparticles (PECNP) composed of cationic poly(l-lysine) (PLL) and various anionic polysaccharides with human vascular endothelial cells (HUVEC) was analyzed.
29882877	7	21	theme	model	1219:1223	arg1	substrates					1225:1234	model substrates	1219:1234	model substrates like tissue culture dishes	1219:1261	PECNP were applied to human umbilical cord vein endothelial cells (HUVEC) in both, the volume phase and immobilized phase at model substrates like tissue culture dishes.
29882877	4	22	theme	adhesive	657:664	arg1	properties					666:675	their cell adhesive properties	646:675	their cell adhesive properties	646:675	In particular PECNP were tested for their cell adhesive properties, their cellular uptake and intracellular localization considering composition and net charge.
29882877	3	23	theme	endothelial	570:580	arg1	HUVEC					589:593	HUVEC	589:593	HUVEC	589:593	Herein the interaction of unloaded polyelectrolyte complex nanoparticles (PECNP) composed of cationic poly(l-lysine) (PLL) and various anionic polysaccharides with human vascular endothelial cells (HUVEC) was analyzed.
29882877	3	23	theme	endothelial	570:580	arg1	cells					582:586	human vascular endothelial cells	555:586	human vascular endothelial cells (HUVEC)	555:594	Herein the interaction of unloaded polyelectrolyte complex nanoparticles (PECNP) composed of cationic poly(l-lysine) (PLL) and various anionic polysaccharides with human vascular endothelial cells (HUVEC) was analyzed.
29882877	16	24	theme	composition	2359:2369	arg1	PLL/CS					2371:2376	the composition PLL/CS	2355:2376	the composition PLL/CS	2355:2376	The negative effects were less obvious with the composition PLL/CS.
29882877	7	25	theme	human	1116:1120	arg1	HUVEC					1161:1165	HUVEC	1161:1165	HUVEC	1161:1165	PECNP were applied to human umbilical cord vein endothelial cells (HUVEC) in both, the volume phase and immobilized phase at model substrates like tissue culture dishes.
29882877	7	25	theme	human	1116:1120	arg1	cells					1154:1158	human umbilical cord vein endothelial cells	1116:1158	human umbilical cord vein endothelial cells (HUVEC) in both, the volume phase and immobilized phase at model substrates like tissue culture dishes	1116:1261	PECNP were applied to human umbilical cord vein endothelial cells (HUVEC) in both, the volume phase and immobilized phase at model substrates like tissue culture dishes.
29882877	3	26	theme	human	555:559	arg1	HUVEC					589:593	HUVEC	589:593	HUVEC	589:593	Herein the interaction of unloaded polyelectrolyte complex nanoparticles (PECNP) composed of cationic poly(l-lysine) (PLL) and various anionic polysaccharides with human vascular endothelial cells (HUVEC) was analyzed.
29882877	3	26	theme	human	555:559	arg1	cells					582:586	human vascular endothelial cells	555:586	human vascular endothelial cells (HUVEC)	555:594	Herein the interaction of unloaded polyelectrolyte complex nanoparticles (PECNP) composed of cationic poly(l-lysine) (PLL) and various anionic polysaccharides with human vascular endothelial cells (HUVEC) was analyzed.
29882877	12	27	theme	further	1816:1822	arg1	processing					1824:1833	apparent further processing	1807:1833	apparent further processing	1807:1833	PECNP were stored in endosomal vesicles in a cumulative manner without apparent further processing.
29882877	11	28	from	PECNP	1583:1587	arg1	phase					1603:1607	the volume phase	1592:1607	the volume phase	1592:1607	PECNP in the volume phase showed no effect of the net charge sign and only minor effects of the composition on the binding and uptake of PECNP at HUVEC.
29882877	9	29	dep	attachment	1434:1443	arg1	systems					1476:1482	the systems PLL/HEP and PLL/DS	1472:1501	the systems PLL/HEP and PLL/DS	1472:1501	Immobilized PECNP reduced attachment of HUVEC, most prominently the systems PLL/HEP and PLL/DS.
29882877	9	29	dep	attachment	1434:1443	arg1	PLL/DS					1496:1501	PLL/DS	1496:1501	PLL/DS	1496:1501	Immobilized PECNP reduced attachment of HUVEC, most prominently the systems PLL/HEP and PLL/DS.
29882877	9	29	dep	attachment	1434:1443	arg1	PLL/HEP					1484:1490	PLL/HEP	1484:1490	PLL/HEP	1484:1490	Immobilized PECNP reduced attachment of HUVEC, most prominently the systems PLL/HEP and PLL/DS.
29882877	14	30	theme	PLL/CS	2121:2126	arg1	composition					2128:2138	PLL/CS composition	2121:2138	PLL/CS composition	2121:2138	Both, in the volume phase and immobilized at the surface, PECNP composed of PLL/HEP and PLL/DS clearly reduced cell proliferation of HUVEC, however without an apparent cytotoxic effect, while PLL/CS composition showed minor impairment.
29882877	0	31	theme	Human	72:76	arg1	Cells					99:103	Human Vascular Endothelial Cells	72:103	Human Vascular Endothelial Cells	72:103	Interaction of Poly(l-lysine)/Polysaccharide Complex Nanoparticles with Human Vascular Endothelial Cells.
29882877	13	32	theme	dividing	1913:1920	arg1	cells					1922:1926	the dividing cells	1909:1926	the dividing cells	1909:1926	During mitosis, internalized PECNP were almost equally distributed among the dividing cells.
29882877	14	33	theme	volume	1942:1947	arg1	phase					1949:1953	the volume phase	1938:1953	the volume phase	1938:1953	Both, in the volume phase and immobilized at the surface, PECNP composed of PLL/HEP and PLL/DS clearly reduced cell proliferation of HUVEC, however without an apparent cytotoxic effect, while PLL/CS composition showed minor impairment.
29882877	0	34	theme	Endothelial	87:97	arg1	Cells					99:103	Human Vascular Endothelial Cells	72:103	Human Vascular Endothelial Cells	72:103	Interaction of Poly(l-lysine)/Polysaccharide Complex Nanoparticles with Human Vascular Endothelial Cells.
29882877	9	35	theme	Immobilized	1408:1418	arg1	PECNP					1420:1424	Immobilized PECNP	1408:1424	Immobilized PECNP	1408:1424	Immobilized PECNP reduced attachment of HUVEC, most prominently the systems PLL/HEP and PLL/DS.
29882877	17	36	theme	biomimetic	2647:2656	arg1	material					2672:2679	biomimetic bone scaffold material	2647:2679	biomimetic bone scaffold material	2647:2679	Since uptake and binding for PLL/HEP was more efficient than for PLL/DS, PECNP of PLL/HEP may be used to deliver growth factors to endothelial cells during vascularization of bone reconstitution material, whereas those of PLL/CS may have an advantage for substituting biomimetic bone scaffold material.
29882877	8	37	theme	growth	1375:1380	arg1	behavior					1382:1389	growth behavior	1375:1389	growth behavior	1375:1389	The attachment of PECNP to the cell surface, their intracellular uptake, and effects on cell proliferation and growth behavior were determined.
29882877	14	38	theme	cytotoxic	2097:2105	arg1	effect					2107:2112	an apparent cytotoxic effect	2085:2112	an apparent cytotoxic effect	2085:2112	Both, in the volume phase and immobilized at the surface, PECNP composed of PLL/HEP and PLL/DS clearly reduced cell proliferation of HUVEC, however without an apparent cytotoxic effect, while PLL/CS composition showed minor impairment.
29882877	3	39	with	polysaccharides	534:548	arg1	HUVEC					589:593	HUVEC	589:593	HUVEC	589:593	Herein the interaction of unloaded polyelectrolyte complex nanoparticles (PECNP) composed of cationic poly(l-lysine) (PLL) and various anionic polysaccharides with human vascular endothelial cells (HUVEC) was analyzed.
29882877	3	39	with	polysaccharides	534:548	arg1	cells					582:586	human vascular endothelial cells	555:586	human vascular endothelial cells (HUVEC)	555:594	Herein the interaction of unloaded polyelectrolyte complex nanoparticles (PECNP) composed of cationic poly(l-lysine) (PLL) and various anionic polysaccharides with human vascular endothelial cells (HUVEC) was analyzed.
29882877	7	40	theme	immobilized	1198:1208	arg1	phase					1210:1214	immobilized phase	1198:1214	immobilized phase at model substrates like tissue culture dishes	1198:1261	PECNP were applied to human umbilical cord vein endothelial cells (HUVEC) in both, the volume phase and immobilized phase at model substrates like tissue culture dishes.
29882877	7	40	theme	immobilized	1198:1208	arg1	both					1171:1174	both	1171:1174	both	1171:1174	PECNP were applied to human umbilical cord vein endothelial cells (HUVEC) in both, the volume phase and immobilized phase at model substrates like tissue culture dishes.
29882877	17	41	theme	bone	2554:2557	arg1	material					2574:2581	bone reconstitution material	2554:2581	bone reconstitution material	2554:2581	Since uptake and binding for PLL/HEP was more efficient than for PLL/DS, PECNP of PLL/HEP may be used to deliver growth factors to endothelial cells during vascularization of bone reconstitution material, whereas those of PLL/CS may have an advantage for substituting biomimetic bone scaffold material.
29882877	1	42	dep	soft	151:154	arg1	regeneration					172:183	tissue regeneration	165:183	tissue regeneration	165:183	Angiogenesis plays an important role in both soft and hard tissue regeneration, which can be modulated by therapeutic drugs.
29882877	11	43	theme	PECNP	1720:1724	arg1	uptake					1710:1715	uptake	1710:1715	uptake	1710:1715	PECNP in the volume phase showed no effect of the net charge sign and only minor effects of the composition on the binding and uptake of PECNP at HUVEC.
29882877	11	43	theme	PECNP	1720:1724	arg1	binding					1698:1704	binding	1698:1704	binding	1698:1704	PECNP in the volume phase showed no effect of the net charge sign and only minor effects of the composition on the binding and uptake of PECNP at HUVEC.
29882877	17	44	theme	material	2574:2581	arg1	vascularization					2535:2549	vascularization	2535:2549	vascularization of bone reconstitution material	2535:2581	Since uptake and binding for PLL/HEP was more efficient than for PLL/DS, PECNP of PLL/HEP may be used to deliver growth factors to endothelial cells during vascularization of bone reconstitution material, whereas those of PLL/CS may have an advantage for substituting biomimetic bone scaffold material.
29882877	4	45	theme	cellular	684:691	arg1	uptake					693:698	their cellular uptake	678:698	their cellular uptake	678:698	In particular PECNP were tested for their cell adhesive properties, their cellular uptake and intracellular localization considering composition and net charge.
29882877	0	46	theme	Poly	15:18	arg1	Nanoparticles					53:65	Poly(l-lysine)/Polysaccharide Complex Nanoparticles	15:65	Poly(l-lysine)/Polysaccharide Complex Nanoparticles	15:65	Interaction of Poly(l-lysine)/Polysaccharide Complex Nanoparticles with Human Vascular Endothelial Cells.
29882877	16	47	theme	negative	2315:2322	arg1	effects					2324:2330	The negative effects	2311:2330	The negative effects	2311:2330	The negative effects were less obvious with the composition PLL/CS.
29882877	16	47	theme	negative	2315:2322	arg1	obvious					2342:2348	obvious	2342:2348	obvious	2342:2348	The negative effects were less obvious with the composition PLL/CS.
29882877	14	48	theme	HUVEC	2062:2066	arg1	proliferation					2045:2057	cell proliferation	2040:2057	cell proliferation of HUVEC	2040:2066	Both, in the volume phase and immobilized at the surface, PECNP composed of PLL/HEP and PLL/DS clearly reduced cell proliferation of HUVEC, however without an apparent cytotoxic effect, while PLL/CS composition showed minor impairment.
29882877	10	49	theme	PECNP	1538:1542	arg1	percentage					1512:1521	A small percentage	1504:1521	A small percentage of immobilized PECNP	1504:1542	A small percentage of immobilized PECNP was taken up by cells during adhesion.
29882877	3	50	with	poly	493:496	arg1	HUVEC					589:593	HUVEC	589:593	HUVEC	589:593	Herein the interaction of unloaded polyelectrolyte complex nanoparticles (PECNP) composed of cationic poly(l-lysine) (PLL) and various anionic polysaccharides with human vascular endothelial cells (HUVEC) was analyzed.
29882877	3	50	with	poly	493:496	arg1	cells					582:586	human vascular endothelial cells	555:586	human vascular endothelial cells (HUVEC)	555:594	Herein the interaction of unloaded polyelectrolyte complex nanoparticles (PECNP) composed of cationic poly(l-lysine) (PLL) and various anionic polysaccharides with human vascular endothelial cells (HUVEC) was analyzed.
29882877	0	51	theme	Nanoparticles	53:65	arg1	Interaction					0:10	Interaction	0:10	Interaction of Poly(l-lysine)/Polysaccharide Complex Nanoparticles with Human Vascular Endothelial Cells	0:103	Interaction of Poly(l-lysine)/Polysaccharide Complex Nanoparticles with Human Vascular Endothelial Cells.
29882877	3	52	theme	nanoparticles	450:462	arg1	interaction					402:412	the interaction	398:412	the interaction of unloaded polyelectrolyte complex nanoparticles (PECNP) composed of cationic poly(l-lysine) (PLL) and various anionic polysaccharides with human vascular endothelial cells (HUVEC)	398:594	Herein the interaction of unloaded polyelectrolyte complex nanoparticles (PECNP) composed of cationic poly(l-lysine) (PLL) and various anionic polysaccharides with human vascular endothelial cells (HUVEC) was analyzed.
29882877	0	53	theme	/Polysaccharide	29:43	arg1	Nanoparticles					53:65	Poly(l-lysine)/Polysaccharide Complex Nanoparticles	15:65	Poly(l-lysine)/Polysaccharide Complex Nanoparticles	15:65	Interaction of Poly(l-lysine)/Polysaccharide Complex Nanoparticles with Human Vascular Endothelial Cells.
29882877	0	54	with	Interaction	0:10	arg1	Cells					99:103	Human Vascular Endothelial Cells	72:103	Human Vascular Endothelial Cells	72:103	Interaction of Poly(l-lysine)/Polysaccharide Complex Nanoparticles with Human Vascular Endothelial Cells.
29882877	3	55	theme	polyelectrolyte	426:440	arg1	nanoparticles					450:462	unloaded polyelectrolyte complex nanoparticles	417:462	unloaded polyelectrolyte complex nanoparticles (PECNP) composed of cationic poly(l-lysine) (PLL) and various anionic polysaccharides with human vascular endothelial cells (HUVEC)	417:594	Herein the interaction of unloaded polyelectrolyte complex nanoparticles (PECNP) composed of cationic poly(l-lysine) (PLL) and various anionic polysaccharides with human vascular endothelial cells (HUVEC) was analyzed.
29882877	3	55	theme	polyelectrolyte	426:440	arg1	PECNP					465:469	PECNP	465:469	PECNP	465:469	Herein the interaction of unloaded polyelectrolyte complex nanoparticles (PECNP) composed of cationic poly(l-lysine) (PLL) and various anionic polysaccharides with human vascular endothelial cells (HUVEC) was analyzed.
29882877	2	56	theme	vascular	349:356	arg1	lumen					358:362	the vascular lumen	345:362	the vascular lumen	345:362	If nanoparticles (NP) are used as vectors for drug delivery, they have to encounter endothelial cells (EC) lining the vascular lumen, if applied intravenously.
29882877	8	57	theme	cell	1295:1298	arg1	surface					1300:1306	the cell surface	1291:1306	the cell surface	1291:1306	The attachment of PECNP to the cell surface, their intracellular uptake, and effects on cell proliferation and growth behavior were determined.
29882877	5	58	theme	cell	806:809	arg1	coating					811:817	cell coating	806:817	cell coating	806:817	PECNP may form a platform for both cell coating and drug delivery.
29882877	7	59	theme	endothelial	1142:1152	arg1	HUVEC					1161:1165	HUVEC	1161:1165	HUVEC	1161:1165	PECNP were applied to human umbilical cord vein endothelial cells (HUVEC) in both, the volume phase and immobilized phase at model substrates like tissue culture dishes.
29882877	7	59	theme	endothelial	1142:1152	arg1	cells					1154:1158	human umbilical cord vein endothelial cells	1116:1158	human umbilical cord vein endothelial cells (HUVEC) in both, the volume phase and immobilized phase at model substrates like tissue culture dishes	1116:1261	PECNP were applied to human umbilical cord vein endothelial cells (HUVEC) in both, the volume phase and immobilized phase at model substrates like tissue culture dishes.
29882877	6	60	from	PLL	857:859	arg1	combination					864:874	combination	864:874	combination with the polysaccharides dextran sulfate (DS), cellulose sulfate (CS) or heparin (HEP)	864:961	PECNP, composed of PLL in combination with the polysaccharides dextran sulfate (DS), cellulose sulfate (CS) or heparin (HEP), either unlabeled or labeled with fluorescein isothiocyanate (FITC) and either with positive or negative net charge were prepared.
29882877	17	61	used	used	2476:2479	arg2	PECNP					2452:2456	PECNP	2452:2456	PECNP of PLL/HEP	2452:2467	Since uptake and binding for PLL/HEP was more efficient than for PLL/DS, PECNP of PLL/HEP may be used to deliver growth factors to endothelial cells during vascularization of bone reconstitution material, whereas those of PLL/CS may have an advantage for substituting biomimetic bone scaffold material.
29882877	2	62	theme	endothelial	315:325	arg1	EC					334:335	EC	334:335	EC	334:335	If nanoparticles (NP) are used as vectors for drug delivery, they have to encounter endothelial cells (EC) lining the vascular lumen, if applied intravenously.
29882877	2	62	theme	endothelial	315:325	arg1	cells					327:331	encounter endothelial cells	305:331	encounter endothelial cells (EC) lining the vascular lumen, if applied intravenously	305:388	If nanoparticles (NP) are used as vectors for drug delivery, they have to encounter endothelial cells (EC) lining the vascular lumen, if applied intravenously.
29882877	7	63	theme	cord	1132:1135	arg1	HUVEC					1161:1165	HUVEC	1161:1165	HUVEC	1161:1165	PECNP were applied to human umbilical cord vein endothelial cells (HUVEC) in both, the volume phase and immobilized phase at model substrates like tissue culture dishes.
29882877	7	63	theme	cord	1132:1135	arg1	cells					1154:1158	human umbilical cord vein endothelial cells	1116:1158	human umbilical cord vein endothelial cells (HUVEC) in both, the volume phase and immobilized phase at model substrates like tissue culture dishes	1116:1261	PECNP were applied to human umbilical cord vein endothelial cells (HUVEC) in both, the volume phase and immobilized phase at model substrates like tissue culture dishes.
29882877	2	64	used	used	257:260	arg2	NP					249:250	NP	249:250	NP	249:250	If nanoparticles (NP) are used as vectors for drug delivery, they have to encounter endothelial cells (EC) lining the vascular lumen, if applied intravenously.
29882877	2	64	used	used	257:260	arg2	nanoparticles					234:246	nanoparticles	234:246	nanoparticles (NP)	234:251	If nanoparticles (NP) are used as vectors for drug delivery, they have to encounter endothelial cells (EC) lining the vascular lumen, if applied intravenously.
29882877	2	64	used	used	257:260	arg2	vectors					265:271	vectors	265:271	vectors for drug delivery	265:289	If nanoparticles (NP) are used as vectors for drug delivery, they have to encounter endothelial cells (EC) lining the vascular lumen, if applied intravenously.
29882877	6	65	theme	positive	1047:1054	arg1	charge					1072:1077	positive or negative net charge	1047:1077	positive or negative net charge	1047:1077	PECNP, composed of PLL in combination with the polysaccharides dextran sulfate (DS), cellulose sulfate (CS) or heparin (HEP), either unlabeled or labeled with fluorescein isothiocyanate (FITC) and either with positive or negative net charge were prepared.
29882877	17	66	theme	endothelial	2510:2520	arg1	cells					2522:2526	endothelial cells	2510:2526	endothelial cells	2510:2526	Since uptake and binding for PLL/HEP was more efficient than for PLL/DS, PECNP of PLL/HEP may be used to deliver growth factors to endothelial cells during vascularization of bone reconstitution material, whereas those of PLL/CS may have an advantage for substituting biomimetic bone scaffold material.
29882877	6	67	theme	net	1068:1070	arg1	charge					1072:1077	positive or negative net charge	1047:1077	positive or negative net charge	1047:1077	PECNP, composed of PLL in combination with the polysaccharides dextran sulfate (DS), cellulose sulfate (CS) or heparin (HEP), either unlabeled or labeled with fluorescein isothiocyanate (FITC) and either with positive or negative net charge were prepared.
29882877	17	68	theme	scaffold	2663:2670	arg1	material					2672:2679	biomimetic bone scaffold material	2647:2679	biomimetic bone scaffold material	2647:2679	Since uptake and binding for PLL/HEP was more efficient than for PLL/DS, PECNP of PLL/HEP may be used to deliver growth factors to endothelial cells during vascularization of bone reconstitution material, whereas those of PLL/CS may have an advantage for substituting biomimetic bone scaffold material.
29882877	3	69	theme	cationic	484:491	arg1	l-lysine					498:505	l-lysine	498:505	l-lysine	498:505	Herein the interaction of unloaded polyelectrolyte complex nanoparticles (PECNP) composed of cationic poly(l-lysine) (PLL) and various anionic polysaccharides with human vascular endothelial cells (HUVEC) was analyzed.
29882877	3	69	theme	cationic	484:491	arg1	PLL					509:511	PLL	509:511	PLL	509:511	Herein the interaction of unloaded polyelectrolyte complex nanoparticles (PECNP) composed of cationic poly(l-lysine) (PLL) and various anionic polysaccharides with human vascular endothelial cells (HUVEC) was analyzed.
29882877	3	69	theme	cationic	484:491	arg1	poly					493:496	cationic poly	484:496	cationic poly(l-lysine) (PLL)	484:512	Herein the interaction of unloaded polyelectrolyte complex nanoparticles (PECNP) composed of cationic poly(l-lysine) (PLL) and various anionic polysaccharides with human vascular endothelial cells (HUVEC) was analyzed.
29882877	7	70	from	cells	1154:1158	arg1	phase					1188:1192	the volume phase	1177:1192	the volume phase	1177:1192	PECNP were applied to human umbilical cord vein endothelial cells (HUVEC) in both, the volume phase and immobilized phase at model substrates like tissue culture dishes.
29882877	7	70	from	cells	1154:1158	arg1	phase					1210:1214	immobilized phase	1198:1214	immobilized phase at model substrates like tissue culture dishes	1198:1261	PECNP were applied to human umbilical cord vein endothelial cells (HUVEC) in both, the volume phase and immobilized phase at model substrates like tissue culture dishes.
29882877	7	70	from	cells	1154:1158	arg1	both					1171:1174	both	1171:1174	both	1171:1174	PECNP were applied to human umbilical cord vein endothelial cells (HUVEC) in both, the volume phase and immobilized phase at model substrates like tissue culture dishes.
29882877	9	71	dep	systems	1476:1482	arg1	systems					1476:1482	the systems PLL/HEP and PLL/DS	1472:1501	the systems PLL/HEP and PLL/DS	1472:1501	Immobilized PECNP reduced attachment of HUVEC, most prominently the systems PLL/HEP and PLL/DS.
29882877	9	71	dep	systems	1476:1482	arg1	PLL/DS					1496:1501	PLL/DS	1496:1501	PLL/DS	1496:1501	Immobilized PECNP reduced attachment of HUVEC, most prominently the systems PLL/HEP and PLL/DS.
29882877	9	71	dep	systems	1476:1482	arg1	PLL/HEP					1484:1490	PLL/HEP	1484:1490	PLL/HEP	1484:1490	Immobilized PECNP reduced attachment of HUVEC, most prominently the systems PLL/HEP and PLL/DS.
29882877	8	72	from	surface	1300:1306	arg1	proliferation					1357:1369	cell proliferation	1352:1369	cell proliferation	1352:1369	The attachment of PECNP to the cell surface, their intracellular uptake, and effects on cell proliferation and growth behavior were determined.
29882877	8	72	from	surface	1300:1306	arg1	behavior					1382:1389	growth behavior	1375:1389	growth behavior	1375:1389	The attachment of PECNP to the cell surface, their intracellular uptake, and effects on cell proliferation and growth behavior were determined.
29882877	12	73	theme	cumulative	1781:1790	arg1	manner					1792:1797	a cumulative manner	1779:1797	a cumulative manner	1779:1797	PECNP were stored in endosomal vesicles in a cumulative manner without apparent further processing.
29882877	6	74	theme	dextran	901:907	arg1	DS					918:919	DS	918:919	DS	918:919	PECNP, composed of PLL in combination with the polysaccharides dextran sulfate (DS), cellulose sulfate (CS) or heparin (HEP), either unlabeled or labeled with fluorescein isothiocyanate (FITC) and either with positive or negative net charge were prepared.
29882877	6	74	theme	dextran	901:907	arg1	sulfate					909:915	the polysaccharides dextran sulfate	881:915	the polysaccharides dextran sulfate (DS)	881:920	PECNP, composed of PLL in combination with the polysaccharides dextran sulfate (DS), cellulose sulfate (CS) or heparin (HEP), either unlabeled or labeled with fluorescein isothiocyanate (FITC) and either with positive or negative net charge were prepared.
29882877	11	75	from	effect	1619:1624	arg1	uptake					1710:1715	uptake	1710:1715	uptake	1710:1715	PECNP in the volume phase showed no effect of the net charge sign and only minor effects of the composition on the binding and uptake of PECNP at HUVEC.
29882877	11	75	from	effect	1619:1624	arg1	binding					1698:1704	binding	1698:1704	binding	1698:1704	PECNP in the volume phase showed no effect of the net charge sign and only minor effects of the composition on the binding and uptake of PECNP at HUVEC.
29882877	11	76	theme	charge	1637:1642	arg1	sign					1644:1647	the net charge sign	1629:1647	the net charge sign	1629:1647	PECNP in the volume phase showed no effect of the net charge sign and only minor effects of the composition on the binding and uptake of PECNP at HUVEC.
29882877	3	77	theme	anionic	526:532	arg1	polysaccharides					534:548	various anionic polysaccharides	518:548	various anionic polysaccharides with human vascular endothelial cells (HUVEC)	518:594	Herein the interaction of unloaded polyelectrolyte complex nanoparticles (PECNP) composed of cationic poly(l-lysine) (PLL) and various anionic polysaccharides with human vascular endothelial cells (HUVEC) was analyzed.
29882877	12	78	theme	endosomal	1757:1765	arg1	vesicles					1767:1774	endosomal vesicles	1757:1774	endosomal vesicles	1757:1774	PECNP were stored in endosomal vesicles in a cumulative manner without apparent further processing.
29882877	8	79	theme	intracellular	1315:1327	arg1	uptake					1329:1334	their intracellular uptake	1309:1334	their intracellular uptake	1309:1334	The attachment of PECNP to the cell surface, their intracellular uptake, and effects on cell proliferation and growth behavior were determined.
29882877	7	80	theme	tissue	1241:1246	arg1	dishes					1256:1261	tissue culture dishes	1241:1261	tissue culture dishes	1241:1261	PECNP were applied to human umbilical cord vein endothelial cells (HUVEC) in both, the volume phase and immobilized phase at model substrates like tissue culture dishes.
29882877	17	81	contain	have	2612:2615	arg1	those					2592:2596	those	2592:2596	those	2592:2596	Since uptake and binding for PLL/HEP was more efficient than for PLL/DS, PECNP of PLL/HEP may be used to deliver growth factors to endothelial cells during vascularization of bone reconstitution material, whereas those of PLL/CS may have an advantage for substituting biomimetic bone scaffold material.
29882877	17	81	contain	have	2612:2615	arg2	advantage					2620:2628	an advantage	2617:2628	an advantage	2617:2628	Since uptake and binding for PLL/HEP was more efficient than for PLL/DS, PECNP of PLL/HEP may be used to deliver growth factors to endothelial cells during vascularization of bone reconstitution material, whereas those of PLL/CS may have an advantage for substituting biomimetic bone scaffold material.
29882877	11	82	theme	minor	1658:1662	arg1	effects					1664:1670	only minor effects	1653:1670	only minor effects of the composition on the binding and uptake of PECNP at HUVEC	1653:1733	PECNP in the volume phase showed no effect of the net charge sign and only minor effects of the composition on the binding and uptake of PECNP at HUVEC.
29882877	15	83	theme	anti-adhesive	2179:2191	arg1	effect					2193:2198	an anti-adhesive effect	2176:2198	an anti-adhesive effect	2176:2198	PECNP have an anti-adhesive effect on HUVEC and are taken up by endothelial cells which may negatively influence the proliferation rate of HUVEC.
29882877	3	84	theme	vascular	561:568	arg1	HUVEC					589:593	HUVEC	589:593	HUVEC	589:593	Herein the interaction of unloaded polyelectrolyte complex nanoparticles (PECNP) composed of cationic poly(l-lysine) (PLL) and various anionic polysaccharides with human vascular endothelial cells (HUVEC) was analyzed.
29882877	3	84	theme	vascular	561:568	arg1	cells					582:586	human vascular endothelial cells	555:586	human vascular endothelial cells (HUVEC)	555:594	Herein the interaction of unloaded polyelectrolyte complex nanoparticles (PECNP) composed of cationic poly(l-lysine) (PLL) and various anionic polysaccharides with human vascular endothelial cells (HUVEC) was analyzed.
29882877	1	85	theme	important	128:136	arg1	role					138:141	an important role	125:141	an important role	125:141	Angiogenesis plays an important role in both soft and hard tissue regeneration, which can be modulated by therapeutic drugs.
29882877	4	86	theme	cell	652:655	arg1	properties					666:675	their cell adhesive properties	646:675	their cell adhesive properties	646:675	In particular PECNP were tested for their cell adhesive properties, their cellular uptake and intracellular localization considering composition and net charge.
29882877	14	87	theme	minor	2147:2151	arg1	impairment					2153:2162	minor impairment	2147:2162	minor impairment	2147:2162	Both, in the volume phase and immobilized at the surface, PECNP composed of PLL/HEP and PLL/DS clearly reduced cell proliferation of HUVEC, however without an apparent cytotoxic effect, while PLL/CS composition showed minor impairment.
29882877	15	88	theme	proliferation	2282:2294	arg1	rate					2296:2299	the proliferation rate	2278:2299	the proliferation rate of HUVEC	2278:2308	PECNP have an anti-adhesive effect on HUVEC and are taken up by endothelial cells which may negatively influence the proliferation rate of HUVEC.
29882877	5	89	theme	drug	823:826	arg1	delivery					828:835	drug delivery	823:835	drug delivery	823:835	PECNP may form a platform for both cell coating and drug delivery.
29882877	8	90	theme	cell	1352:1355	arg1	proliferation					1357:1369	cell proliferation	1352:1369	cell proliferation	1352:1369	The attachment of PECNP to the cell surface, their intracellular uptake, and effects on cell proliferation and growth behavior were determined.
29882877	11	91	from	HUVEC	1729:1733	arg1	uptake					1710:1715	uptake	1710:1715	uptake	1710:1715	PECNP in the volume phase showed no effect of the net charge sign and only minor effects of the composition on the binding and uptake of PECNP at HUVEC.
29882877	11	91	from	HUVEC	1729:1733	arg1	binding					1698:1704	binding	1698:1704	binding	1698:1704	PECNP in the volume phase showed no effect of the net charge sign and only minor effects of the composition on the binding and uptake of PECNP at HUVEC.
29882877	4	92	theme	particular	613:622	arg1	PECNP					624:628	particular PECNP	613:628	particular PECNP	613:628	In particular PECNP were tested for their cell adhesive properties, their cellular uptake and intracellular localization considering composition and net charge.
29882877	11	93	theme	volume	1596:1601	arg1	phase					1603:1607	the volume phase	1592:1607	the volume phase	1592:1607	PECNP in the volume phase showed no effect of the net charge sign and only minor effects of the composition on the binding and uptake of PECNP at HUVEC.
29882877	0	94	theme	Vascular	78:85	arg1	Cells					99:103	Human Vascular Endothelial Cells	72:103	Human Vascular Endothelial Cells	72:103	Interaction of Poly(l-lysine)/Polysaccharide Complex Nanoparticles with Human Vascular Endothelial Cells.
29882877	6	95	theme	cellulose	923:931	arg1	CS					942:943	CS	942:943	CS	942:943	PECNP, composed of PLL in combination with the polysaccharides dextran sulfate (DS), cellulose sulfate (CS) or heparin (HEP), either unlabeled or labeled with fluorescein isothiocyanate (FITC) and either with positive or negative net charge were prepared.
29882877	6	95	theme	cellulose	923:931	arg1	sulfate					933:939	cellulose sulfate	923:939	cellulose sulfate (CS)	923:944	PECNP, composed of PLL in combination with the polysaccharides dextran sulfate (DS), cellulose sulfate (CS) or heparin (HEP), either unlabeled or labeled with fluorescein isothiocyanate (FITC) and either with positive or negative net charge were prepared.
29882877	8	96	from	effects	1341:1347	arg1	proliferation					1357:1369	cell proliferation	1352:1369	cell proliferation	1352:1369	The attachment of PECNP to the cell surface, their intracellular uptake, and effects on cell proliferation and growth behavior were determined.
29882877	8	96	from	effects	1341:1347	arg1	behavior					1382:1389	growth behavior	1375:1389	growth behavior	1375:1389	The attachment of PECNP to the cell surface, their intracellular uptake, and effects on cell proliferation and growth behavior were determined.
29882877	4	97	dep	tested	635:640	arg1	considering					731:741	considering	731:741	considering composition and net charge	731:768	In particular PECNP were tested for their cell adhesive properties, their cellular uptake and intracellular localization considering composition and net charge.
29882877	3	98	theme	complex	442:448	arg1	nanoparticles					450:462	unloaded polyelectrolyte complex nanoparticles	417:462	unloaded polyelectrolyte complex nanoparticles (PECNP) composed of cationic poly(l-lysine) (PLL) and various anionic polysaccharides with human vascular endothelial cells (HUVEC)	417:594	Herein the interaction of unloaded polyelectrolyte complex nanoparticles (PECNP) composed of cationic poly(l-lysine) (PLL) and various anionic polysaccharides with human vascular endothelial cells (HUVEC) was analyzed.
29882877	3	98	theme	complex	442:448	arg1	PECNP					465:469	PECNP	465:469	PECNP	465:469	Herein the interaction of unloaded polyelectrolyte complex nanoparticles (PECNP) composed of cationic poly(l-lysine) (PLL) and various anionic polysaccharides with human vascular endothelial cells (HUVEC) was analyzed.
29882877	15	99	contain	have	2171:2174	arg1	PECNP					2165:2169	PECNP	2165:2169	PECNP	2165:2169	PECNP have an anti-adhesive effect on HUVEC and are taken up by endothelial cells which may negatively influence the proliferation rate of HUVEC.
29882877	15	99	contain	have	2171:2174	arg2	effect					2193:2198	an anti-adhesive effect	2176:2198	an anti-adhesive effect	2176:2198	PECNP have an anti-adhesive effect on HUVEC and are taken up by endothelial cells which may negatively influence the proliferation rate of HUVEC.
29882877	17	100	theme	bone	2658:2661	arg1	material					2672:2679	biomimetic bone scaffold material	2647:2679	biomimetic bone scaffold material	2647:2679	Since uptake and binding for PLL/HEP was more efficient than for PLL/DS, PECNP of PLL/HEP may be used to deliver growth factors to endothelial cells during vascularization of bone reconstitution material, whereas those of PLL/CS may have an advantage for substituting biomimetic bone scaffold material.
29882877	4	101	theme	intracellular	704:716	arg1	localization					718:729	intracellular localization	704:729	intracellular localization	704:729	In particular PECNP were tested for their cell adhesive properties, their cellular uptake and intracellular localization considering composition and net charge.
29882877	14	102	theme	apparent	2088:2095	arg1	effect					2107:2112	an apparent cytotoxic effect	2085:2112	an apparent cytotoxic effect	2085:2112	Both, in the volume phase and immobilized at the surface, PECNP composed of PLL/HEP and PLL/DS clearly reduced cell proliferation of HUVEC, however without an apparent cytotoxic effect, while PLL/CS composition showed minor impairment.
29882877	11	103	dep	binding	1698:1704	arg1	the					1694:1696	the	1694:1696	the	1694:1696	PECNP in the volume phase showed no effect of the net charge sign and only minor effects of the composition on the binding and uptake of PECNP at HUVEC.
29882877	7	104	from	substrates	1225:1234	arg1	phase					1188:1192	the volume phase	1177:1192	the volume phase	1177:1192	PECNP were applied to human umbilical cord vein endothelial cells (HUVEC) in both, the volume phase and immobilized phase at model substrates like tissue culture dishes.
29882877	7	104	from	substrates	1225:1234	arg1	phase					1210:1214	immobilized phase	1198:1214	immobilized phase at model substrates like tissue culture dishes	1198:1261	PECNP were applied to human umbilical cord vein endothelial cells (HUVEC) in both, the volume phase and immobilized phase at model substrates like tissue culture dishes.
29882877	7	104	from	substrates	1225:1234	arg1	both					1171:1174	both	1171:1174	both	1171:1174	PECNP were applied to human umbilical cord vein endothelial cells (HUVEC) in both, the volume phase and immobilized phase at model substrates like tissue culture dishes.
29882877	7	105	theme	volume	1181:1186	arg1	phase					1188:1192	the volume phase	1177:1192	the volume phase	1177:1192	PECNP were applied to human umbilical cord vein endothelial cells (HUVEC) in both, the volume phase and immobilized phase at model substrates like tissue culture dishes.
29882877	7	105	theme	volume	1181:1186	arg1	both					1171:1174	both	1171:1174	both	1171:1174	PECNP were applied to human umbilical cord vein endothelial cells (HUVEC) in both, the volume phase and immobilized phase at model substrates like tissue culture dishes.
29882877	17	106	theme	reconstitution	2559:2572	arg1	material					2574:2581	bone reconstitution material	2554:2581	bone reconstitution material	2554:2581	Since uptake and binding for PLL/HEP was more efficient than for PLL/DS, PECNP of PLL/HEP may be used to deliver growth factors to endothelial cells during vascularization of bone reconstitution material, whereas those of PLL/CS may have an advantage for substituting biomimetic bone scaffold material.
29882877	8	107	attach	attachment	1268:1277	arg1	surface					1300:1306	the cell surface	1291:1306	the cell surface	1291:1306	The attachment of PECNP to the cell surface, their intracellular uptake, and effects on cell proliferation and growth behavior were determined.
29882877	8	107	attach	attachment	1268:1277	arg2	PECNP					1282:1286	PECNP	1282:1286	PECNP	1282:1286	The attachment of PECNP to the cell surface, their intracellular uptake, and effects on cell proliferation and growth behavior were determined.
29882877	8	107	attach	attachment	1268:1277	arg1	uptake					1329:1334	their intracellular uptake	1309:1334	their intracellular uptake	1309:1334	The attachment of PECNP to the cell surface, their intracellular uptake, and effects on cell proliferation and growth behavior were determined.
29882877	8	107	attach	attachment	1268:1277	arg1	effects					1341:1347	effects	1341:1347	effects on cell proliferation and growth behavior	1341:1389	The attachment of PECNP to the cell surface, their intracellular uptake, and effects on cell proliferation and growth behavior were determined.
29882877	4	108	theme	net	759:761	arg1	charge					763:768	net charge	759:768	net charge	759:768	In particular PECNP were tested for their cell adhesive properties, their cellular uptake and intracellular localization considering composition and net charge.
29882877	9	109	theme	HUVEC	1448:1452	arg1	attachment					1434:1443	attachment	1434:1443	attachment	1434:1443	Immobilized PECNP reduced attachment of HUVEC, most prominently the systems PLL/HEP and PLL/DS.
29882877	10	110	theme	immobilized	1526:1536	arg1	PECNP					1538:1542	immobilized PECNP	1526:1542	immobilized PECNP	1526:1542	A small percentage of immobilized PECNP was taken up by cells during adhesion.
29882877	6	111	dep	either	964:969	arg1	unlabeled					971:979	unlabeled	971:979	unlabeled	971:979	PECNP, composed of PLL in combination with the polysaccharides dextran sulfate (DS), cellulose sulfate (CS) or heparin (HEP), either unlabeled or labeled with fluorescein isothiocyanate (FITC) and either with positive or negative net charge were prepared.
29882877	3	112	theme	unloaded	417:424	arg1	nanoparticles					450:462	unloaded polyelectrolyte complex nanoparticles	417:462	unloaded polyelectrolyte complex nanoparticles (PECNP) composed of cationic poly(l-lysine) (PLL) and various anionic polysaccharides with human vascular endothelial cells (HUVEC)	417:594	Herein the interaction of unloaded polyelectrolyte complex nanoparticles (PECNP) composed of cationic poly(l-lysine) (PLL) and various anionic polysaccharides with human vascular endothelial cells (HUVEC) was analyzed.
29882877	3	112	theme	unloaded	417:424	arg1	PECNP					465:469	PECNP	465:469	PECNP	465:469	Herein the interaction of unloaded polyelectrolyte complex nanoparticles (PECNP) composed of cationic poly(l-lysine) (PLL) and various anionic polysaccharides with human vascular endothelial cells (HUVEC) was analyzed.
29882877	8	113	from	uptake	1329:1334	arg1	proliferation					1357:1369	cell proliferation	1352:1369	cell proliferation	1352:1369	The attachment of PECNP to the cell surface, their intracellular uptake, and effects on cell proliferation and growth behavior were determined.
29882877	8	113	from	uptake	1329:1334	arg1	behavior					1382:1389	growth behavior	1375:1389	growth behavior	1375:1389	The attachment of PECNP to the cell surface, their intracellular uptake, and effects on cell proliferation and growth behavior were determined.
29882877	0	114	theme	Complex	45:51	arg1	Nanoparticles					53:65	Poly(l-lysine)/Polysaccharide Complex Nanoparticles	15:65	Poly(l-lysine)/Polysaccharide Complex Nanoparticles	15:65	Interaction of Poly(l-lysine)/Polysaccharide Complex Nanoparticles with Human Vascular Endothelial Cells.
29882877	15	115	theme	endothelial	2229:2239	arg1	cells					2241:2245	endothelial cells	2229:2245	endothelial cells which may negatively influence the proliferation rate of HUVEC	2229:2308	PECNP have an anti-adhesive effect on HUVEC and are taken up by endothelial cells which may negatively influence the proliferation rate of HUVEC.
29882877	13	116	theme	internalized	1852:1863	arg1	PECNP					1865:1869	internalized PECNP	1852:1869	internalized PECNP	1852:1869	During mitosis, internalized PECNP were almost equally distributed among the dividing cells.
29882877	2	117	dep	cells	327:331	arg1	lining					338:343	lining	338:343	lining	338:343	If nanoparticles (NP) are used as vectors for drug delivery, they have to encounter endothelial cells (EC) lining the vascular lumen, if applied intravenously.
29882877	8	118	theme	PECNP	1282:1286	arg1	attachment					1268:1277	The attachment	1264:1277	The attachment of PECNP to the cell surface, their intracellular uptake, and effects on cell proliferation and growth behavior	1264:1389	The attachment of PECNP to the cell surface, their intracellular uptake, and effects on cell proliferation and growth behavior were determined.
29882877	11	119	theme	composition	1679:1689	arg1	effect					1619:1624	no effect	1616:1624	no effect of the net charge sign	1616:1647	PECNP in the volume phase showed no effect of the net charge sign and only minor effects of the composition on the binding and uptake of PECNP at HUVEC.
29882877	11	119	theme	composition	1679:1689	arg1	effects					1664:1670	only minor effects	1653:1670	only minor effects of the composition on the binding and uptake of PECNP at HUVEC	1653:1733	PECNP in the volume phase showed no effect of the net charge sign and only minor effects of the composition on the binding and uptake of PECNP at HUVEC.
29882877	1	120	theme	therapeutic	212:222	arg1	drugs					224:228	therapeutic drugs	212:228	therapeutic drugs	212:228	Angiogenesis plays an important role in both soft and hard tissue regeneration, which can be modulated by therapeutic drugs.
29882877	7	121	theme	vein	1137:1140	arg1	HUVEC					1161:1165	HUVEC	1161:1165	HUVEC	1161:1165	PECNP were applied to human umbilical cord vein endothelial cells (HUVEC) in both, the volume phase and immobilized phase at model substrates like tissue culture dishes.
29882877	7	121	theme	vein	1137:1140	arg1	cells					1154:1158	human umbilical cord vein endothelial cells	1116:1158	human umbilical cord vein endothelial cells (HUVEC) in both, the volume phase and immobilized phase at model substrates like tissue culture dishes	1116:1261	PECNP were applied to human umbilical cord vein endothelial cells (HUVEC) in both, the volume phase and immobilized phase at model substrates like tissue culture dishes.
29882877	6	122	dep	PECNP	838:842	arg1	either					964:969	either	964:969	either	964:969	PECNP, composed of PLL in combination with the polysaccharides dextran sulfate (DS), cellulose sulfate (CS) or heparin (HEP), either unlabeled or labeled with fluorescein isothiocyanate (FITC) and either with positive or negative net charge were prepared.
29882877	6	122	dep	PECNP	838:842	arg1	labeled					984:990	labeled	984:990	labeled with fluorescein isothiocyanate (FITC) and either with positive or negative net charge	984:1077	PECNP, composed of PLL in combination with the polysaccharides dextran sulfate (DS), cellulose sulfate (CS) or heparin (HEP), either unlabeled or labeled with fluorescein isothiocyanate (FITC) and either with positive or negative net charge were prepared.
30286318	9	0	attach	present	1650:1656	arg1	cytoplasm					1665:1673	the cytoplasm	1661:1673	the cytoplasm of P. bursaria	1661:1688	The amount of glycosaminoglycans on the cell wall may decrease in C. variabilis present in the cytoplasm of P. bursaria.
30286318	9	0	attach	present	1650:1656	arg2	variabilis					1639:1648	C. variabilis	1636:1648	C. variabilis present in the cytoplasm of P. bursaria	1636:1688	The amount of glycosaminoglycans on the cell wall may decrease in C. variabilis present in the cytoplasm of P. bursaria.
30286318	1	1	theme	host-free	324:332	arg1	conditions					334:343	host-free conditions	324:343	host-free conditions	324:343	Chlorella variabilis, a symbiotic alga, is usually present in the cytoplasm of Paramecium bursaria, although it can be cultured in host-free conditions.
30286318	7	2	theme	calcofluor	1171:1180	arg1	intensity					1195:1203	calcofluor fluorescence intensity	1171:1203	calcofluor fluorescence intensity	1171:1203	Because treatment with cellulase showed no effect on calcofluor fluorescence intensity, we consider that cellulose is not majorly responsible for the stainability of calcofluor.
30286318	5	3	theme	host	921:924	arg1	cells					926:930	Paramecium host cells	910:930	Paramecium host cells	910:930	When the algae were re-introduced into Paramecium host cells, calcofluor fluorescence intensity reduced by about 50%.
30286318	9	4	from	present	1650:1656	arg1	cytoplasm					1665:1673	the cytoplasm	1661:1673	the cytoplasm of P. bursaria	1661:1688	The amount of glycosaminoglycans on the cell wall may decrease in C. variabilis present in the cytoplasm of P. bursaria.
30286318	8	5	theme	symbiotic	1378:1386	arg1	conditions					1388:1397	the free-living and symbiotic conditions	1358:1397	conditions	1388:1397	Staining intensities of FITC-WGA and FITC-LFA were similar in the free-living and symbiotic conditions, suggesting that N-acetylglucosamine and sialic acid are also not responsible for the reduction in the stainability of calcofluor associated with intracellular symbiosis.
30286318	0	6	from	changes	22:28	arg1	ultrastructure					33:46	ultrastructure	33:46	ultrastructure	33:46	Endosymbiosis-related changes in ultrastructure and chemical composition of Chlorella variabilis (Archaeplastida, Chlorophyta) cell wall in Paramecium bursaria (Ciliophora, Oligohymenophorea).
30286318	7	7	theme	fluorescence	1182:1193	arg1	intensity					1195:1203	calcofluor fluorescence intensity	1171:1203	calcofluor fluorescence intensity	1171:1203	Because treatment with cellulase showed no effect on calcofluor fluorescence intensity, we consider that cellulose is not majorly responsible for the stainability of calcofluor.
30286318	2	8	theme	Morphological	346:358	arg1	properties					373:382	Morphological and chemical properties	346:382	Morphological and chemical properties of its cell wall	346:399	Morphological and chemical properties of its cell wall were compared between its free-living and symbiotic states.
30286318	4	9	with	treatment	717:725	arg1	reagents					750:757	three fluorescent reagents	732:757	three fluorescent reagents (calcofluor white M2R, FITC-WGA, and FITC-LFA) having specific binding affinities to different polysaccharides	732:868	Chemical properties of the cell wall were examined by treatment with three fluorescent reagents (calcofluor white M2R, FITC-WGA, and FITC-LFA) having specific binding affinities to different polysaccharides.
30286318	4	9	with	treatment	717:725	arg1	FITC-WGA					782:789	FITC-WGA	782:789	FITC-WGA	782:789	Chemical properties of the cell wall were examined by treatment with three fluorescent reagents (calcofluor white M2R, FITC-WGA, and FITC-LFA) having specific binding affinities to different polysaccharides.
30286318	4	9	with	treatment	717:725	arg1	FITC-LFA					796:803	FITC-LFA	796:803	FITC-LFA	796:803	Chemical properties of the cell wall were examined by treatment with three fluorescent reagents (calcofluor white M2R, FITC-WGA, and FITC-LFA) having specific binding affinities to different polysaccharides.
30286318	4	9	with	treatment	717:725	arg1	M2R					777:779	calcofluor white M2R	760:779	calcofluor white M2R	760:779	Chemical properties of the cell wall were examined by treatment with three fluorescent reagents (calcofluor white M2R, FITC-WGA, and FITC-LFA) having specific binding affinities to different polysaccharides.
30286318	1	10	attach	present	244:250	arg2	alga					227:230	a symbiotic alga	215:230	a symbiotic alga	215:230	Chlorella variabilis, a symbiotic alga, is usually present in the cytoplasm of Paramecium bursaria, although it can be cultured in host-free conditions.
30286318	1	10	attach	present	244:250	arg1	cytoplasm					259:267	the cytoplasm	255:267	the cytoplasm of Paramecium bursaria	255:290	Chlorella variabilis, a symbiotic alga, is usually present in the cytoplasm of Paramecium bursaria, although it can be cultured in host-free conditions.
30286318	1	10	attach	present	244:250	arg2	variabilis					203:212	Chlorella variabilis	193:212	Chlorella variabilis	193:212	Chlorella variabilis, a symbiotic alga, is usually present in the cytoplasm of Paramecium bursaria, although it can be cultured in host-free conditions.
30286318	3	11	theme	wall	566:569	arg1	thickness					571:579	the cell wall thickness	557:579	the cell wall thickness of symbiotic C. variabilis	557:606	Transmission electron microscopy (quick-freezing and freeze-substitution methods) revealed that the cell wall thickness of symbiotic C. variabilis was reduced to about half that of the free-living one.
30286318	9	12	theme	C.	1636:1637	arg1	variabilis					1639:1648	C. variabilis	1636:1648	C. variabilis present in the cytoplasm of P. bursaria	1636:1688	The amount of glycosaminoglycans on the cell wall may decrease in C. variabilis present in the cytoplasm of P. bursaria.
30286318	3	13	dep	quick-freezing	495:508	arg1	methods					534:540	methods	534:540	methods	534:540	Transmission electron microscopy (quick-freezing and freeze-substitution methods) revealed that the cell wall thickness of symbiotic C. variabilis was reduced to about half that of the free-living one.
30286318	8	14	from	reduction	1485:1493	arg1	stainability					1502:1513	the stainability	1498:1513	the stainability of calcofluor associated with intracellular symbiosis	1498:1567	Staining intensities of FITC-WGA and FITC-LFA were similar in the free-living and symbiotic conditions, suggesting that N-acetylglucosamine and sialic acid are also not responsible for the reduction in the stainability of calcofluor associated with intracellular symbiosis.
30286318	4	15	dep	reagents	750:757	arg1	reagents					750:757	three fluorescent reagents	732:757	three fluorescent reagents (calcofluor white M2R, FITC-WGA, and FITC-LFA) having specific binding affinities to different polysaccharides	732:868	Chemical properties of the cell wall were examined by treatment with three fluorescent reagents (calcofluor white M2R, FITC-WGA, and FITC-LFA) having specific binding affinities to different polysaccharides.
30286318	4	15	dep	reagents	750:757	arg1	FITC-WGA					782:789	FITC-WGA	782:789	FITC-WGA	782:789	Chemical properties of the cell wall were examined by treatment with three fluorescent reagents (calcofluor white M2R, FITC-WGA, and FITC-LFA) having specific binding affinities to different polysaccharides.
30286318	4	15	dep	reagents	750:757	arg1	FITC-LFA					796:803	FITC-LFA	796:803	FITC-LFA	796:803	Chemical properties of the cell wall were examined by treatment with three fluorescent reagents (calcofluor white M2R, FITC-WGA, and FITC-LFA) having specific binding affinities to different polysaccharides.
30286318	4	15	dep	reagents	750:757	arg1	M2R					777:779	calcofluor white M2R	760:779	calcofluor white M2R	760:779	Chemical properties of the cell wall were examined by treatment with three fluorescent reagents (calcofluor white M2R, FITC-WGA, and FITC-LFA) having specific binding affinities to different polysaccharides.
30286318	0	16	theme	cell	127:130	arg1	wall					132:135	cell wall	127:135	cell wall in Paramecium bursaria (Ciliophora, Oligohymenophorea)	127:190	Endosymbiosis-related changes in ultrastructure and chemical composition of Chlorella variabilis (Archaeplastida, Chlorophyta) cell wall in Paramecium bursaria (Ciliophora, Oligohymenophorea).
30286318	0	17	from	composition	61:71	arg1	ultrastructure					33:46	ultrastructure	33:46	ultrastructure	33:46	Endosymbiosis-related changes in ultrastructure and chemical composition of Chlorella variabilis (Archaeplastida, Chlorophyta) cell wall in Paramecium bursaria (Ciliophora, Oligohymenophorea).
30286318	8	18	theme	FITC-LFA	1333:1340	arg1	intensities					1305:1315	Staining intensities	1296:1315	Staining intensities of FITC-WGA and FITC-LFA	1296:1340	Staining intensities of FITC-WGA and FITC-LFA were similar in the free-living and symbiotic conditions, suggesting that N-acetylglucosamine and sialic acid are also not responsible for the reduction in the stainability of calcofluor associated with intracellular symbiosis.
30286318	4	19	theme	cell	690:693	arg1	wall					695:698	the cell wall	686:698	the cell wall	686:698	Chemical properties of the cell wall were examined by treatment with three fluorescent reagents (calcofluor white M2R, FITC-WGA, and FITC-LFA) having specific binding affinities to different polysaccharides.
30286318	2	20	theme	wall	396:399	arg1	properties					373:382	Morphological and chemical properties	346:382	Morphological and chemical properties of its cell wall	346:399	Morphological and chemical properties of its cell wall were compared between its free-living and symbiotic states.
30286318	6	21	theme	β-d-glucopyranose	1007:1023	arg1	glycosaminoglycans					1098:1115	glycosaminoglycans	1098:1115	glycosaminoglycans	1098:1115	Calcofluor stains β-d-glucopyranose polysaccharides such as cellulose, N-acetylglucosamine, sialic acid, and glycosaminoglycans.
30286318	6	21	theme	β-d-glucopyranose	1007:1023	arg1	N-acetylglucosamine					1060:1078	N-acetylglucosamine	1060:1078	N-acetylglucosamine	1060:1078	Calcofluor stains β-d-glucopyranose polysaccharides such as cellulose, N-acetylglucosamine, sialic acid, and glycosaminoglycans.
30286318	6	21	theme	β-d-glucopyranose	1007:1023	arg1	polysaccharides					1025:1039	β-d-glucopyranose polysaccharides	1007:1039	β-d-glucopyranose polysaccharides such as cellulose, N-acetylglucosamine, sialic acid, and glycosaminoglycans	1007:1115	Calcofluor stains β-d-glucopyranose polysaccharides such as cellulose, N-acetylglucosamine, sialic acid, and glycosaminoglycans.
30286318	6	21	theme	β-d-glucopyranose	1007:1023	arg1	cellulose					1049:1057	cellulose	1049:1057	cellulose	1049:1057	Calcofluor stains β-d-glucopyranose polysaccharides such as cellulose, N-acetylglucosamine, sialic acid, and glycosaminoglycans.
30286318	6	21	theme	β-d-glucopyranose	1007:1023	arg1	acid					1088:1091	sialic acid	1081:1091	sialic acid	1081:1091	Calcofluor stains β-d-glucopyranose polysaccharides such as cellulose, N-acetylglucosamine, sialic acid, and glycosaminoglycans.
30286318	4	22	theme	different	844:852	arg1	polysaccharides					854:868	different polysaccharides	844:868	different polysaccharides	844:868	Chemical properties of the cell wall were examined by treatment with three fluorescent reagents (calcofluor white M2R, FITC-WGA, and FITC-LFA) having specific binding affinities to different polysaccharides.
30286318	2	23	theme	symbiotic	443:451	arg1	states					453:458	its free-living and symbiotic states	423:458	its free-living and symbiotic states	423:458	Morphological and chemical properties of its cell wall were compared between its free-living and symbiotic states.
30286318	2	24	theme	cell	391:394	arg1	wall					396:399	its cell wall	387:399	its cell wall	387:399	Morphological and chemical properties of its cell wall were compared between its free-living and symbiotic states.
30286318	7	25	theme	calcofluor	1284:1293	arg1	stainability					1268:1279	the stainability	1264:1279	the stainability of calcofluor	1264:1293	Because treatment with cellulase showed no effect on calcofluor fluorescence intensity, we consider that cellulose is not majorly responsible for the stainability of calcofluor.
30286318	3	26	theme	C.	594:595	arg1	variabilis					597:606	symbiotic C. variabilis	584:606	symbiotic C. variabilis	584:606	Transmission electron microscopy (quick-freezing and freeze-substitution methods) revealed that the cell wall thickness of symbiotic C. variabilis was reduced to about half that of the free-living one.
30286318	1	27	theme	Chlorella	193:201	arg1	variabilis					203:212	Chlorella variabilis	193:212	Chlorella variabilis	193:212	Chlorella variabilis, a symbiotic alga, is usually present in the cytoplasm of Paramecium bursaria, although it can be cultured in host-free conditions.
30286318	1	27	theme	Chlorella	193:201	arg1	alga					227:230	a symbiotic alga	215:230	a symbiotic alga	215:230	Chlorella variabilis, a symbiotic alga, is usually present in the cytoplasm of Paramecium bursaria, although it can be cultured in host-free conditions.
30286318	0	28	theme	Endosymbiosis-related	0:20	arg1	changes					22:28	Endosymbiosis-related changes	0:28	Endosymbiosis-related changes in ultrastructure	0:46	Endosymbiosis-related changes in ultrastructure and chemical composition of Chlorella variabilis (Archaeplastida, Chlorophyta) cell wall in Paramecium bursaria (Ciliophora, Oligohymenophorea).
30286318	1	29	theme	Paramecium	272:281	arg1	bursaria					283:290	Paramecium bursaria	272:290	Paramecium bursaria	272:290	Chlorella variabilis, a symbiotic alga, is usually present in the cytoplasm of Paramecium bursaria, although it can be cultured in host-free conditions.
30286318	9	30	theme	bursaria	1681:1688	arg1	cytoplasm					1665:1673	the cytoplasm	1661:1673	the cytoplasm of P. bursaria	1661:1688	The amount of glycosaminoglycans on the cell wall may decrease in C. variabilis present in the cytoplasm of P. bursaria.
30286318	8	31	from	conditions	1388:1397	arg1	similar					1347:1353	similar	1347:1353	similar	1347:1353	Staining intensities of FITC-WGA and FITC-LFA were similar in the free-living and symbiotic conditions, suggesting that N-acetylglucosamine and sialic acid are also not responsible for the reduction in the stainability of calcofluor associated with intracellular symbiosis.
30286318	3	32	theme	variabilis	597:606	arg1	thickness					571:579	the cell wall thickness	557:579	the cell wall thickness of symbiotic C. variabilis	557:606	Transmission electron microscopy (quick-freezing and freeze-substitution methods) revealed that the cell wall thickness of symbiotic C. variabilis was reduced to about half that of the free-living one.
30286318	8	33	theme	sialic	1440:1445	arg1	acid					1447:1450	sialic acid	1440:1450	sialic acid	1440:1450	Staining intensities of FITC-WGA and FITC-LFA were similar in the free-living and symbiotic conditions, suggesting that N-acetylglucosamine and sialic acid are also not responsible for the reduction in the stainability of calcofluor associated with intracellular symbiosis.
30286318	8	34	theme	calcofluor	1518:1527	arg1	stainability					1502:1513	the stainability	1498:1513	the stainability of calcofluor associated with intracellular symbiosis	1498:1567	Staining intensities of FITC-WGA and FITC-LFA were similar in the free-living and symbiotic conditions, suggesting that N-acetylglucosamine and sialic acid are also not responsible for the reduction in the stainability of calcofluor associated with intracellular symbiosis.
30286318	1	35	theme	bursaria	283:290	arg1	cytoplasm					259:267	the cytoplasm	255:267	the cytoplasm of Paramecium bursaria	255:290	Chlorella variabilis, a symbiotic alga, is usually present in the cytoplasm of Paramecium bursaria, although it can be cultured in host-free conditions.
30286318	1	36	from	cytoplasm	259:267	arg1	present					244:250	present	244:250	present	244:250	Chlorella variabilis, a symbiotic alga, is usually present in the cytoplasm of Paramecium bursaria, although it can be cultured in host-free conditions.
30286318	4	37	theme	white	771:775	arg1	reagents					750:757	three fluorescent reagents	732:757	three fluorescent reagents (calcofluor white M2R, FITC-WGA, and FITC-LFA) having specific binding affinities to different polysaccharides	732:868	Chemical properties of the cell wall were examined by treatment with three fluorescent reagents (calcofluor white M2R, FITC-WGA, and FITC-LFA) having specific binding affinities to different polysaccharides.
30286318	4	37	theme	white	771:775	arg1	M2R					777:779	calcofluor white M2R	760:779	calcofluor white M2R	760:779	Chemical properties of the cell wall were examined by treatment with three fluorescent reagents (calcofluor white M2R, FITC-WGA, and FITC-LFA) having specific binding affinities to different polysaccharides.
30286318	9	38	theme	P.	1678:1679	arg1	bursaria					1681:1688	P. bursaria	1678:1688	P. bursaria	1678:1688	The amount of glycosaminoglycans on the cell wall may decrease in C. variabilis present in the cytoplasm of P. bursaria.
30286318	3	39	theme	electron	474:481	arg1	microscopy					483:492	Transmission electron microscopy	461:492	Transmission electron microscopy (quick-freezing and freeze-substitution methods)	461:541	Transmission electron microscopy (quick-freezing and freeze-substitution methods) revealed that the cell wall thickness of symbiotic C. variabilis was reduced to about half that of the free-living one.
30286318	2	40	theme	free-living	427:437	arg1	states					453:458	its free-living and symbiotic states	423:458	its free-living and symbiotic states	423:458	Morphological and chemical properties of its cell wall were compared between its free-living and symbiotic states.
30286318	8	41	theme	intracellular	1545:1557	arg1	symbiosis					1559:1567	intracellular symbiosis	1545:1567	intracellular symbiosis	1545:1567	Staining intensities of FITC-WGA and FITC-LFA were similar in the free-living and symbiotic conditions, suggesting that N-acetylglucosamine and sialic acid are also not responsible for the reduction in the stainability of calcofluor associated with intracellular symbiosis.
30286318	3	42	theme	symbiotic	584:592	arg1	variabilis					597:606	symbiotic C. variabilis	584:606	symbiotic C. variabilis	584:606	Transmission electron microscopy (quick-freezing and freeze-substitution methods) revealed that the cell wall thickness of symbiotic C. variabilis was reduced to about half that of the free-living one.
30286318	9	43	theme	cell	1610:1613	arg1	wall					1615:1618	the cell wall	1606:1618	the cell wall	1606:1618	The amount of glycosaminoglycans on the cell wall may decrease in C. variabilis present in the cytoplasm of P. bursaria.
30286318	0	44	theme	chemical	52:59	arg1	composition					61:71	chemical composition	52:71	chemical composition of Chlorella variabilis (Archaeplastida, Chlorophyta)	52:125	Endosymbiosis-related changes in ultrastructure and chemical composition of Chlorella variabilis (Archaeplastida, Chlorophyta) cell wall in Paramecium bursaria (Ciliophora, Oligohymenophorea).
30286318	5	45	theme	calcofluor	933:942	arg1	intensity					957:965	calcofluor fluorescence intensity	933:965	calcofluor fluorescence intensity	933:965	When the algae were re-introduced into Paramecium host cells, calcofluor fluorescence intensity reduced by about 50%.
30286318	9	46	theme	glycosaminoglycans	1584:1601	arg1	glycosaminoglycans					1584:1601	glycosaminoglycans	1584:1601	glycosaminoglycans	1584:1601	The amount of glycosaminoglycans on the cell wall may decrease in C. variabilis present in the cytoplasm of P. bursaria.
30286318	9	46	theme	glycosaminoglycans	1584:1601	arg1	amount					1574:1579	The amount	1570:1579	The amount of glycosaminoglycans on the cell wall	1570:1618	The amount of glycosaminoglycans on the cell wall may decrease in C. variabilis present in the cytoplasm of P. bursaria.
30286318	4	47	theme	Chemical	663:670	arg1	properties					672:681	Chemical properties	663:681	Chemical properties of the cell wall	663:698	Chemical properties of the cell wall were examined by treatment with three fluorescent reagents (calcofluor white M2R, FITC-WGA, and FITC-LFA) having specific binding affinities to different polysaccharides.
30286318	4	48	theme	binding	822:828	arg1	affinities					830:839	specific binding affinities	813:839	specific binding affinities	813:839	Chemical properties of the cell wall were examined by treatment with three fluorescent reagents (calcofluor white M2R, FITC-WGA, and FITC-LFA) having specific binding affinities to different polysaccharides.
30286318	1	49	theme	symbiotic	217:225	arg1	variabilis					203:212	Chlorella variabilis	193:212	Chlorella variabilis	193:212	Chlorella variabilis, a symbiotic alga, is usually present in the cytoplasm of Paramecium bursaria, although it can be cultured in host-free conditions.
30286318	1	49	theme	symbiotic	217:225	arg1	alga					227:230	a symbiotic alga	215:230	a symbiotic alga	215:230	Chlorella variabilis, a symbiotic alga, is usually present in the cytoplasm of Paramecium bursaria, although it can be cultured in host-free conditions.
30286318	0	50	theme	Paramecium	140:149	arg1	bursaria					151:158	Paramecium bursaria	140:158	Paramecium bursaria (Ciliophora, Oligohymenophorea)	140:190	Endosymbiosis-related changes in ultrastructure and chemical composition of Chlorella variabilis (Archaeplastida, Chlorophyta) cell wall in Paramecium bursaria (Ciliophora, Oligohymenophorea).
30286318	0	50	theme	Paramecium	140:149	arg1	Oligohymenophorea					173:189	Oligohymenophorea	173:189	Oligohymenophorea	173:189	Endosymbiosis-related changes in ultrastructure and chemical composition of Chlorella variabilis (Archaeplastida, Chlorophyta) cell wall in Paramecium bursaria (Ciliophora, Oligohymenophorea).
30286318	3	51	theme	free-living	646:656	arg1	one					658:660	the free-living one	642:660	the free-living one	642:660	Transmission electron microscopy (quick-freezing and freeze-substitution methods) revealed that the cell wall thickness of symbiotic C. variabilis was reduced to about half that of the free-living one.
30286318	4	52	theme	specific	813:820	arg1	affinities					830:839	specific binding affinities	813:839	specific binding affinities	813:839	Chemical properties of the cell wall were examined by treatment with three fluorescent reagents (calcofluor white M2R, FITC-WGA, and FITC-LFA) having specific binding affinities to different polysaccharides.
30286318	8	53	theme	free-living	1362:1372	arg1	conditions					1388:1397	the free-living and symbiotic conditions	1358:1397	conditions	1388:1397	Staining intensities of FITC-WGA and FITC-LFA were similar in the free-living and symbiotic conditions, suggesting that N-acetylglucosamine and sialic acid are also not responsible for the reduction in the stainability of calcofluor associated with intracellular symbiosis.
30286318	3	54	theme	Transmission	461:472	arg1	microscopy					483:492	Transmission electron microscopy	461:492	Transmission electron microscopy (quick-freezing and freeze-substitution methods)	461:541	Transmission electron microscopy (quick-freezing and freeze-substitution methods) revealed that the cell wall thickness of symbiotic C. variabilis was reduced to about half that of the free-living one.
30286318	7	55	with	treatment	1126:1134	arg1	cellulase					1141:1149	cellulase	1141:1149	cellulase	1141:1149	Because treatment with cellulase showed no effect on calcofluor fluorescence intensity, we consider that cellulose is not majorly responsible for the stainability of calcofluor.
30286318	3	56	theme	one	658:660	arg1	half					629:632	about half	623:632	about half that of the free-living one	623:660	Transmission electron microscopy (quick-freezing and freeze-substitution methods) revealed that the cell wall thickness of symbiotic C. variabilis was reduced to about half that of the free-living one.
30286318	1	57	located	present	244:250	arg2	alga					227:230	a symbiotic alga	215:230	a symbiotic alga	215:230	Chlorella variabilis, a symbiotic alga, is usually present in the cytoplasm of Paramecium bursaria, although it can be cultured in host-free conditions.
30286318	1	57	located	present	244:250	arg2	variabilis					203:212	Chlorella variabilis	193:212	Chlorella variabilis	193:212	Chlorella variabilis, a symbiotic alga, is usually present in the cytoplasm of Paramecium bursaria, although it can be cultured in host-free conditions.
30286318	1	57	located	present	244:250	arg1	cytoplasm					259:267	the cytoplasm	255:267	the cytoplasm of Paramecium bursaria	255:290	Chlorella variabilis, a symbiotic alga, is usually present in the cytoplasm of Paramecium bursaria, although it can be cultured in host-free conditions.
30286318	9	58	from	amount	1574:1579	arg1	wall					1615:1618	the cell wall	1606:1618	the cell wall	1606:1618	The amount of glycosaminoglycans on the cell wall may decrease in C. variabilis present in the cytoplasm of P. bursaria.
30286318	1	59	from	present	244:250	arg1	cytoplasm					259:267	the cytoplasm	255:267	the cytoplasm of Paramecium bursaria	255:290	Chlorella variabilis, a symbiotic alga, is usually present in the cytoplasm of Paramecium bursaria, although it can be cultured in host-free conditions.
30286318	5	60	theme	fluorescence	944:955	arg1	intensity					957:965	calcofluor fluorescence intensity	933:965	calcofluor fluorescence intensity	933:965	When the algae were re-introduced into Paramecium host cells, calcofluor fluorescence intensity reduced by about 50%.
30286318	9	61	from	cytoplasm	1665:1673	arg1	present					1650:1656	present	1650:1656	present	1650:1656	The amount of glycosaminoglycans on the cell wall may decrease in C. variabilis present in the cytoplasm of P. bursaria.
30286318	8	62	theme	FITC-WGA	1320:1327	arg1	intensities					1305:1315	Staining intensities	1296:1315	Staining intensities of FITC-WGA and FITC-LFA	1296:1340	Staining intensities of FITC-WGA and FITC-LFA were similar in the free-living and symbiotic conditions, suggesting that N-acetylglucosamine and sialic acid are also not responsible for the reduction in the stainability of calcofluor associated with intracellular symbiosis.
30286318	4	63	theme	fluorescent	738:748	arg1	reagents					750:757	three fluorescent reagents	732:757	three fluorescent reagents (calcofluor white M2R, FITC-WGA, and FITC-LFA) having specific binding affinities to different polysaccharides	732:868	Chemical properties of the cell wall were examined by treatment with three fluorescent reagents (calcofluor white M2R, FITC-WGA, and FITC-LFA) having specific binding affinities to different polysaccharides.
30286318	4	63	theme	fluorescent	738:748	arg1	FITC-WGA					782:789	FITC-WGA	782:789	FITC-WGA	782:789	Chemical properties of the cell wall were examined by treatment with three fluorescent reagents (calcofluor white M2R, FITC-WGA, and FITC-LFA) having specific binding affinities to different polysaccharides.
30286318	4	63	theme	fluorescent	738:748	arg1	FITC-LFA					796:803	FITC-LFA	796:803	FITC-LFA	796:803	Chemical properties of the cell wall were examined by treatment with three fluorescent reagents (calcofluor white M2R, FITC-WGA, and FITC-LFA) having specific binding affinities to different polysaccharides.
30286318	4	63	theme	fluorescent	738:748	arg1	M2R					777:779	calcofluor white M2R	760:779	calcofluor white M2R	760:779	Chemical properties of the cell wall were examined by treatment with three fluorescent reagents (calcofluor white M2R, FITC-WGA, and FITC-LFA) having specific binding affinities to different polysaccharides.
30286318	3	64	theme	cell	561:564	arg1	thickness					571:579	the cell wall thickness	557:579	the cell wall thickness of symbiotic C. variabilis	557:606	Transmission electron microscopy (quick-freezing and freeze-substitution methods) revealed that the cell wall thickness of symbiotic C. variabilis was reduced to about half that of the free-living one.
30286318	9	65	theme	present	1650:1656	arg1	variabilis					1639:1648	C. variabilis	1636:1648	C. variabilis present in the cytoplasm of P. bursaria	1636:1688	The amount of glycosaminoglycans on the cell wall may decrease in C. variabilis present in the cytoplasm of P. bursaria.
30286318	8	66	from	similar	1347:1353	arg1	conditions					1388:1397	the free-living and symbiotic conditions	1358:1397	conditions	1388:1397	Staining intensities of FITC-WGA and FITC-LFA were similar in the free-living and symbiotic conditions, suggesting that N-acetylglucosamine and sialic acid are also not responsible for the reduction in the stainability of calcofluor associated with intracellular symbiosis.
30286318	4	67	theme	wall	695:698	arg1	properties					672:681	Chemical properties	663:681	Chemical properties of the cell wall	663:698	Chemical properties of the cell wall were examined by treatment with three fluorescent reagents (calcofluor white M2R, FITC-WGA, and FITC-LFA) having specific binding affinities to different polysaccharides.
30286318	4	68	contain	having	806:811	arg1	reagents					750:757	three fluorescent reagents	732:757	three fluorescent reagents (calcofluor white M2R, FITC-WGA, and FITC-LFA) having specific binding affinities to different polysaccharides	732:868	Chemical properties of the cell wall were examined by treatment with three fluorescent reagents (calcofluor white M2R, FITC-WGA, and FITC-LFA) having specific binding affinities to different polysaccharides.
30286318	4	68	contain	having	806:811	arg2	affinities					830:839	specific binding affinities	813:839	specific binding affinities	813:839	Chemical properties of the cell wall were examined by treatment with three fluorescent reagents (calcofluor white M2R, FITC-WGA, and FITC-LFA) having specific binding affinities to different polysaccharides.
30286318	4	68	contain	having	806:811	arg1	FITC-WGA					782:789	FITC-WGA	782:789	FITC-WGA	782:789	Chemical properties of the cell wall were examined by treatment with three fluorescent reagents (calcofluor white M2R, FITC-WGA, and FITC-LFA) having specific binding affinities to different polysaccharides.
30286318	4	68	contain	having	806:811	arg1	FITC-LFA					796:803	FITC-LFA	796:803	FITC-LFA	796:803	Chemical properties of the cell wall were examined by treatment with three fluorescent reagents (calcofluor white M2R, FITC-WGA, and FITC-LFA) having specific binding affinities to different polysaccharides.
30286318	4	68	contain	having	806:811	arg1	M2R					777:779	calcofluor white M2R	760:779	calcofluor white M2R	760:779	Chemical properties of the cell wall were examined by treatment with three fluorescent reagents (calcofluor white M2R, FITC-WGA, and FITC-LFA) having specific binding affinities to different polysaccharides.
30286318	0	69	theme	variabilis	86:95	arg1	composition					61:71	chemical composition	52:71	chemical composition of Chlorella variabilis (Archaeplastida, Chlorophyta)	52:125	Endosymbiosis-related changes in ultrastructure and chemical composition of Chlorella variabilis (Archaeplastida, Chlorophyta) cell wall in Paramecium bursaria (Ciliophora, Oligohymenophorea).
30286318	0	69	theme	variabilis	86:95	arg1	changes					22:28	Endosymbiosis-related changes	0:28	Endosymbiosis-related changes in ultrastructure	0:46	Endosymbiosis-related changes in ultrastructure and chemical composition of Chlorella variabilis (Archaeplastida, Chlorophyta) cell wall in Paramecium bursaria (Ciliophora, Oligohymenophorea).
30286318	6	70	theme	sialic	1081:1086	arg1	acid					1088:1091	sialic acid	1081:1091	sialic acid	1081:1091	Calcofluor stains β-d-glucopyranose polysaccharides such as cellulose, N-acetylglucosamine, sialic acid, and glycosaminoglycans.
30286318	0	71	dep	changes	22:28	arg1	wall					132:135	cell wall	127:135	cell wall in Paramecium bursaria (Ciliophora, Oligohymenophorea)	127:190	Endosymbiosis-related changes in ultrastructure and chemical composition of Chlorella variabilis (Archaeplastida, Chlorophyta) cell wall in Paramecium bursaria (Ciliophora, Oligohymenophorea).
30286318	3	72	dep	half	629:632	arg1	that					634:637	that	634:637	that	634:637	Transmission electron microscopy (quick-freezing and freeze-substitution methods) revealed that the cell wall thickness of symbiotic C. variabilis was reduced to about half that of the free-living one.
30286318	5	73	theme	Paramecium	910:919	arg1	cells					926:930	Paramecium host cells	910:930	Paramecium host cells	910:930	When the algae were re-introduced into Paramecium host cells, calcofluor fluorescence intensity reduced by about 50%.
30286318	8	74	theme	Staining	1296:1303	arg1	intensities					1305:1315	Staining intensities	1296:1315	Staining intensities of FITC-WGA and FITC-LFA	1296:1340	Staining intensities of FITC-WGA and FITC-LFA were similar in the free-living and symbiotic conditions, suggesting that N-acetylglucosamine and sialic acid are also not responsible for the reduction in the stainability of calcofluor associated with intracellular symbiosis.
30286318	0	75	theme	Chlorella	76:84	arg1	Chlorophyta					114:124	Chlorophyta	114:124	Chlorophyta	114:124	Endosymbiosis-related changes in ultrastructure and chemical composition of Chlorella variabilis (Archaeplastida, Chlorophyta) cell wall in Paramecium bursaria (Ciliophora, Oligohymenophorea).
30286318	0	75	theme	Chlorella	76:84	arg1	variabilis					86:95	Chlorella variabilis	76:95	Chlorella variabilis (Archaeplastida, Chlorophyta)	76:125	Endosymbiosis-related changes in ultrastructure and chemical composition of Chlorella variabilis (Archaeplastida, Chlorophyta) cell wall in Paramecium bursaria (Ciliophora, Oligohymenophorea).
30286318	3	76	dep	microscopy	483:492	arg1	freeze-substitution					514:532	freeze-substitution	514:532	freeze-substitution	514:532	Transmission electron microscopy (quick-freezing and freeze-substitution methods) revealed that the cell wall thickness of symbiotic C. variabilis was reduced to about half that of the free-living one.
30286318	3	76	dep	microscopy	483:492	arg1	quick-freezing					495:508	quick-freezing	495:508	quick-freezing	495:508	Transmission electron microscopy (quick-freezing and freeze-substitution methods) revealed that the cell wall thickness of symbiotic C. variabilis was reduced to about half that of the free-living one.
30286318	2	77	theme	chemical	364:371	arg1	properties					373:382	Morphological and chemical properties	346:382	Morphological and chemical properties of its cell wall	346:399	Morphological and chemical properties of its cell wall were compared between its free-living and symbiotic states.
30286318	0	78	from	wall	132:135	arg1	bursaria					151:158	Paramecium bursaria	140:158	Paramecium bursaria (Ciliophora, Oligohymenophorea)	140:190	Endosymbiosis-related changes in ultrastructure and chemical composition of Chlorella variabilis (Archaeplastida, Chlorophyta) cell wall in Paramecium bursaria (Ciliophora, Oligohymenophorea).
30286318	0	78	from	wall	132:135	arg1	Oligohymenophorea					173:189	Oligohymenophorea	173:189	Oligohymenophorea	173:189	Endosymbiosis-related changes in ultrastructure and chemical composition of Chlorella variabilis (Archaeplastida, Chlorophyta) cell wall in Paramecium bursaria (Ciliophora, Oligohymenophorea).
31726594	7	0	theme	typical	1106:1112	arg1	cycle					1124:1128	one typical operating cycle	1102:1128	one typical operating cycle	1102:1128	In one typical operating cycle, however, Mn2+ retarded nitrification and the degradation of aniline, while promoted denitrification.
31726594	1	1	theme	pollutant	308:316	arg1	efficiency					326:335	pollutant removal efficiency	308:335	pollutant removal efficiency	308:335	In this study, the effect of high concentration of Mn2+ on the aerobic granular sludge (AGS) systems for aniline wastewater treatment was systematically investigated in terms of AGS formation and pollutant removal efficiency.
31726594	6	2	theme	demand	974:979	arg1	efficiency					944:953	high overall removal efficiency	923:953	high overall removal efficiency of chemical oxygen demand, NH4+-N, and total nitrogen	923:1007	The reactors showed high overall removal efficiency of chemical oxygen demand, NH4+-N, and total nitrogen with average concentrations below 40, 1.0, and 19 mg L-1, respectively, in the effluents.
31726594	3	3	theme	Mn2+	483:486	arg1	prolonged					517:525	prolonged	517:525	prolonged	517:525	In the presence of Mn2+, the time to granulation was prolonged from 23 d to 30 d due to the toxicity of the high concentration of Mn2+.
31726594	3	3	theme	Mn2+	483:486	arg1	time					493:496	the time	489:496	the time to granulation	489:511	In the presence of Mn2+, the time to granulation was prolonged from 23 d to 30 d due to the toxicity of the high concentration of Mn2+.
31726594	6	4	theme	high	923:926	arg1	efficiency					944:953	high overall removal efficiency	923:953	high overall removal efficiency of chemical oxygen demand, NH4+-N, and total nitrogen	923:1007	The reactors showed high overall removal efficiency of chemical oxygen demand, NH4+-N, and total nitrogen with average concentrations below 40, 1.0, and 19 mg L-1, respectively, in the effluents.
31726594	7	5	theme	operating	1114:1122	arg1	cycle					1124:1128	one typical operating cycle	1102:1128	one typical operating cycle	1102:1128	In one typical operating cycle, however, Mn2+ retarded nitrification and the degradation of aniline, while promoted denitrification.
31726594	1	6	theme	removal	318:324	arg1	efficiency					326:335	pollutant removal efficiency	308:335	pollutant removal efficiency	308:335	In this study, the effect of high concentration of Mn2+ on the aerobic granular sludge (AGS) systems for aniline wastewater treatment was systematically investigated in terms of AGS formation and pollutant removal efficiency.
31726594	5	7	from	reactors	812:819	arg1	substances					774:783	The extracellular polymeric substances	746:783	The extracellular polymeric substances of the granules in the two reactors	746:819	The extracellular polymeric substances of the granules in the two reactors had similar protein compositions, but some functional groups increased with Mn2+.
31726594	1	8	theme	high	141:144	arg1	concentration					146:158	high concentration	141:158	high concentration of Mn2+	141:166	In this study, the effect of high concentration of Mn2+ on the aerobic granular sludge (AGS) systems for aniline wastewater treatment was systematically investigated in terms of AGS formation and pollutant removal efficiency.
31726594	1	9	theme	aniline	217:223	arg1	treatment					236:244	aniline wastewater treatment	217:244	aniline wastewater treatment	217:244	In this study, the effect of high concentration of Mn2+ on the aerobic granular sludge (AGS) systems for aniline wastewater treatment was systematically investigated in terms of AGS formation and pollutant removal efficiency.
31726594	0	10	theme	wastewater	90:99	arg1	treatment					101:109	aniline wastewater treatment	82:109	aniline wastewater treatment	82:109	High concentration of Mn2+ has multiple influences on aerobic granular sludge for aniline wastewater treatment.
31726594	1	11	theme	efficiency	326:335	arg1	terms					281:285	terms	281:285	terms of AGS formation and pollutant removal efficiency	281:335	In this study, the effect of high concentration of Mn2+ on the aerobic granular sludge (AGS) systems for aniline wastewater treatment was systematically investigated in terms of AGS formation and pollutant removal efficiency.
31726594	8	12	theme	community	1246:1254	arg1	analysis					1256:1263	The microbial community analysis	1232:1263	The microbial community analysis	1232:1263	The microbial community analysis revealed that the growth of Terrisporobacter, Pseudomonas, and many other bacteria responsible for aniline degradation was inhibited by Mn2+, and so were the strains involved in nitrification.
31726594	6	13	theme	oxygen	967:972	arg1	demand					974:979	chemical oxygen demand	958:979	chemical oxygen demand	958:979	The reactors showed high overall removal efficiency of chemical oxygen demand, NH4+-N, and total nitrogen with average concentrations below 40, 1.0, and 19 mg L-1, respectively, in the effluents.
31726594	1	14	theme	wastewater	225:234	arg1	treatment					236:244	aniline wastewater treatment	217:244	aniline wastewater treatment	217:244	In this study, the effect of high concentration of Mn2+ on the aerobic granular sludge (AGS) systems for aniline wastewater treatment was systematically investigated in terms of AGS formation and pollutant removal efficiency.
31726594	0	15	theme	aniline	82:88	arg1	treatment					101:109	aniline wastewater treatment	82:109	aniline wastewater treatment	82:109	High concentration of Mn2+ has multiple influences on aerobic granular sludge for aniline wastewater treatment.
31726594	8	16	dep	and	1407:1409	arg1	so					1411:1412	so	1411:1412	so	1411:1412	The microbial community analysis revealed that the growth of Terrisporobacter, Pseudomonas, and many other bacteria responsible for aniline degradation was inhibited by Mn2+, and so were the strains involved in nitrification.
31726594	0	17	contain	has	27:29	arg2	influences					40:49	multiple influences	31:49	multiple influences	31:49	High concentration of Mn2+ has multiple influences on aerobic granular sludge for aniline wastewater treatment.
31726594	0	17	contain	has	27:29	arg1	concentration					5:17	High concentration	0:17	High concentration of Mn2+	0:25	High concentration of Mn2+ has multiple influences on aerobic granular sludge for aniline wastewater treatment.
31726594	8	18	theme	microbial	1236:1244	arg1	community					1246:1254	The microbial community	1232:1254	The microbial community analysis	1232:1263	The microbial community analysis revealed that the growth of Terrisporobacter, Pseudomonas, and many other bacteria responsible for aniline degradation was inhibited by Mn2+, and so were the strains involved in nitrification.
31726594	5	19	from	granules	792:799	arg1	reactors					812:819	the two reactors	804:819	the two reactors	804:819	The extracellular polymeric substances of the granules in the two reactors had similar protein compositions, but some functional groups increased with Mn2+.
31726594	5	20	theme	extracellular	750:762	arg1	substances					774:783	The extracellular polymeric substances	746:783	The extracellular polymeric substances of the granules in the two reactors	746:819	The extracellular polymeric substances of the granules in the two reactors had similar protein compositions, but some functional groups increased with Mn2+.
31726594	1	21	from	effect	131:136	arg1	systems					205:211	the aerobic granular sludge (AGS) systems	171:211	the aerobic granular sludge (AGS) systems for aniline wastewater treatment	171:244	In this study, the effect of high concentration of Mn2+ on the aerobic granular sludge (AGS) systems for aniline wastewater treatment was systematically investigated in terms of AGS formation and pollutant removal efficiency.
31726594	5	22	theme	granules	792:799	arg1	substances					774:783	The extracellular polymeric substances	746:783	The extracellular polymeric substances of the granules in the two reactors	746:819	The extracellular polymeric substances of the granules in the two reactors had similar protein compositions, but some functional groups increased with Mn2+.
31726594	1	23	theme	concentration	146:158	arg1	effect					131:136	the effect	127:136	the effect of high concentration of Mn2+ on the aerobic granular sludge (AGS) systems for aniline wastewater treatment	127:244	In this study, the effect of high concentration of Mn2+ on the aerobic granular sludge (AGS) systems for aniline wastewater treatment was systematically investigated in terms of AGS formation and pollutant removal efficiency.
31726594	6	24	theme	nitrogen	1000:1007	arg1	efficiency					944:953	high overall removal efficiency	923:953	high overall removal efficiency of chemical oxygen demand, NH4+-N, and total nitrogen	923:1007	The reactors showed high overall removal efficiency of chemical oxygen demand, NH4+-N, and total nitrogen with average concentrations below 40, 1.0, and 19 mg L-1, respectively, in the effluents.
31726594	6	25	theme	total	994:998	arg1	nitrogen					1000:1007	total nitrogen	994:1007	total nitrogen	994:1007	The reactors showed high overall removal efficiency of chemical oxygen demand, NH4+-N, and total nitrogen with average concentrations below 40, 1.0, and 19 mg L-1, respectively, in the effluents.
31726594	3	26	dep	Mn2+	483:486	arg1	presence					471:478	presence	471:478	presence	471:478	In the presence of Mn2+, the time to granulation was prolonged from 23 d to 30 d due to the toxicity of the high concentration of Mn2+.
31726594	3	26	dep	Mn2+	483:486	arg1	the					467:469	the	467:469	the	467:469	In the presence of Mn2+, the time to granulation was prolonged from 23 d to 30 d due to the toxicity of the high concentration of Mn2+.
31726594	1	27	theme	Mn2+	163:166	arg1	concentration					146:158	high concentration	141:158	high concentration of Mn2+	141:166	In this study, the effect of high concentration of Mn2+ on the aerobic granular sludge (AGS) systems for aniline wastewater treatment was systematically investigated in terms of AGS formation and pollutant removal efficiency.
31726594	0	28	theme	High	0:3	arg1	concentration					5:17	High concentration	0:17	High concentration of Mn2+	0:25	High concentration of Mn2+ has multiple influences on aerobic granular sludge for aniline wastewater treatment.
31726594	2	29	theme	batch	362:366	arg1	reactors					368:375	Two parallel sequencing batch reactors	338:375	Two parallel sequencing batch reactors	338:375	Two parallel sequencing batch reactors were operated to treat the aniline-rich wastewater with and without 20 mg L-1 of Mn2+.
31726594	0	30	theme	Mn2+	22:25	arg1	concentration					5:17	High concentration	0:17	High concentration of Mn2+	0:25	High concentration of Mn2+ has multiple influences on aerobic granular sludge for aniline wastewater treatment.
31726594	8	31	theme	aniline	1364:1370	arg1	degradation					1372:1382	aniline degradation	1364:1382	aniline degradation	1364:1382	The microbial community analysis revealed that the growth of Terrisporobacter, Pseudomonas, and many other bacteria responsible for aniline degradation was inhibited by Mn2+, and so were the strains involved in nitrification.
31726594	4	32	theme	larger	692:697	arg1	870 μm					705:710	870 μm	705:710	870 μm	705:710	However, the mature granules with Mn2+ produced more protein and polysaccharides, and had a larger size (870 μm) than that without Mn2+ (740 μm).
31726594	4	32	theme	larger	692:697	arg1	size					699:702	a larger size	690:702	a larger size (870 μm)	690:711	However, the mature granules with Mn2+ produced more protein and polysaccharides, and had a larger size (870 μm) than that without Mn2+ (740 μm).
31726594	2	33	theme	aniline-rich	404:415	arg1	wastewater					417:426	the aniline-rich wastewater	400:426	the aniline-rich wastewater with and without 20 mg L-1 of Mn2+	400:461	Two parallel sequencing batch reactors were operated to treat the aniline-rich wastewater with and without 20 mg L-1 of Mn2+.
31726594	7	34	theme	aniline	1191:1197	arg1	nitrification					1154:1166	nitrification	1154:1166	nitrification	1154:1166	In one typical operating cycle, however, Mn2+ retarded nitrification and the degradation of aniline, while promoted denitrification.
31726594	7	34	theme	aniline	1191:1197	arg1	degradation					1176:1186	the degradation	1172:1186	the degradation of aniline	1172:1197	In one typical operating cycle, however, Mn2+ retarded nitrification and the degradation of aniline, while promoted denitrification.
31726594	4	35	with	granules	620:627	arg1	Mn2+					634:637	Mn2+	634:637	Mn2+	634:637	However, the mature granules with Mn2+ produced more protein and polysaccharides, and had a larger size (870 μm) than that without Mn2+ (740 μm).
31726594	3	36	dep	30 d	540:543	arg1	to					537:538	to	537:538	to	537:538	In the presence of Mn2+, the time to granulation was prolonged from 23 d to 30 d due to the toxicity of the high concentration of Mn2+.
31726594	1	37	theme	aerobic	175:181	arg1	systems					205:211	the aerobic granular sludge (AGS) systems	171:211	the aerobic granular sludge (AGS) systems for aniline wastewater treatment	171:244	In this study, the effect of high concentration of Mn2+ on the aerobic granular sludge (AGS) systems for aniline wastewater treatment was systematically investigated in terms of AGS formation and pollutant removal efficiency.
31726594	0	38	theme	multiple	31:38	arg1	influences					40:49	multiple influences	31:49	multiple influences	31:49	High concentration of Mn2+ has multiple influences on aerobic granular sludge for aniline wastewater treatment.
31726594	4	39	contain	had	686:688	arg2	870 μm					705:710	870 μm	705:710	870 μm	705:710	However, the mature granules with Mn2+ produced more protein and polysaccharides, and had a larger size (870 μm) than that without Mn2+ (740 μm).
31726594	4	39	contain	had	686:688	arg2	size					699:702	a larger size	690:702	a larger size (870 μm)	690:711	However, the mature granules with Mn2+ produced more protein and polysaccharides, and had a larger size (870 μm) than that without Mn2+ (740 μm).
31726594	4	39	contain	had	686:688	arg1	granules					620:627	the mature granules	609:627	the mature granules with Mn2+	609:637	However, the mature granules with Mn2+ produced more protein and polysaccharides, and had a larger size (870 μm) than that without Mn2+ (740 μm).
31726594	5	40	theme	polymeric	764:772	arg1	substances					774:783	The extracellular polymeric substances	746:783	The extracellular polymeric substances of the granules in the two reactors	746:819	The extracellular polymeric substances of the granules in the two reactors had similar protein compositions, but some functional groups increased with Mn2+.
31726594	4	41	theme	mature	613:618	arg1	granules					620:627	the mature granules	609:627	the mature granules with Mn2+	609:637	However, the mature granules with Mn2+ produced more protein and polysaccharides, and had a larger size (870 μm) than that without Mn2+ (740 μm).
31726594	1	42	theme	granular	183:190	arg1	systems					205:211	the aerobic granular sludge (AGS) systems	171:211	the aerobic granular sludge (AGS) systems for aniline wastewater treatment	171:244	In this study, the effect of high concentration of Mn2+ on the aerobic granular sludge (AGS) systems for aniline wastewater treatment was systematically investigated in terms of AGS formation and pollutant removal efficiency.
31726594	2	43	theme	Mn2+	458:461	arg1	20 mg L-1					445:453	20 mg L-1	445:453	20 mg L-1 of Mn2+	445:461	Two parallel sequencing batch reactors were operated to treat the aniline-rich wastewater with and without 20 mg L-1 of Mn2+.
31726594	8	44	theme	Terrisporobacter	1293:1308	arg1	growth					1283:1288	the growth	1279:1288	the growth of Terrisporobacter, Pseudomonas, and many other bacteria responsible for aniline degradation	1279:1382	The microbial community analysis revealed that the growth of Terrisporobacter, Pseudomonas, and many other bacteria responsible for aniline degradation was inhibited by Mn2+, and so were the strains involved in nitrification.
31726594	1	45	theme	sludge	192:197	arg1	systems					205:211	the aerobic granular sludge (AGS) systems	171:211	the aerobic granular sludge (AGS) systems for aniline wastewater treatment	171:244	In this study, the effect of high concentration of Mn2+ on the aerobic granular sludge (AGS) systems for aniline wastewater treatment was systematically investigated in terms of AGS formation and pollutant removal efficiency.
31726594	8	46	dep	Terrisporobacter	1293:1308	arg1	Pseudomonas					1311:1321	Terrisporobacter, Pseudomonas, and many other bacteria responsible for aniline degradation	1293:1382	Pseudomonas	1311:1321	The microbial community analysis revealed that the growth of Terrisporobacter, Pseudomonas, and many other bacteria responsible for aniline degradation was inhibited by Mn2+, and so were the strains involved in nitrification.
31726594	3	47	theme	Mn2+	594:597	arg1	concentration					577:589	the high concentration	568:589	the high concentration of Mn2+	568:597	In the presence of Mn2+, the time to granulation was prolonged from 23 d to 30 d due to the toxicity of the high concentration of Mn2+.
31726594	9	48	theme	denitrifying	1502:1513	arg1	bacteria					1515:1522	denitrifying bacteria	1502:1522	denitrifying bacteria	1502:1522	In contrast, Mn2+ facilitated the growth of denitrifying bacteria.
31726594	5	49	from	substances	774:783	arg1	reactors					812:819	the two reactors	804:819	the two reactors	804:819	The extracellular polymeric substances of the granules in the two reactors had similar protein compositions, but some functional groups increased with Mn2+.
31726594	5	50	theme	similar	825:831	arg1	compositions					841:852	similar protein compositions	825:852	similar protein compositions	825:852	The extracellular polymeric substances of the granules in the two reactors had similar protein compositions, but some functional groups increased with Mn2+.
31726594	1	51	theme	AGS	290:292	arg1	formation					294:302	AGS formation	290:302	AGS formation	290:302	In this study, the effect of high concentration of Mn2+ on the aerobic granular sludge (AGS) systems for aniline wastewater treatment was systematically investigated in terms of AGS formation and pollutant removal efficiency.
31726594	6	52	theme	NH4+-N	982:987	arg1	efficiency					944:953	high overall removal efficiency	923:953	high overall removal efficiency of chemical oxygen demand, NH4+-N, and total nitrogen	923:1007	The reactors showed high overall removal efficiency of chemical oxygen demand, NH4+-N, and total nitrogen with average concentrations below 40, 1.0, and 19 mg L-1, respectively, in the effluents.
31726594	9	53	theme	bacteria	1515:1522	arg1	growth					1492:1497	the growth	1488:1497	the growth of denitrifying bacteria	1488:1522	In contrast, Mn2+ facilitated the growth of denitrifying bacteria.
31726594	5	54	theme	protein	833:839	arg1	compositions					841:852	similar protein compositions	825:852	similar protein compositions	825:852	The extracellular polymeric substances of the granules in the two reactors had similar protein compositions, but some functional groups increased with Mn2+.
31726594	8	55	theme	responsible	1348:1358	arg1	bacteria					1339:1346	many other bacteria	1328:1346	many other bacteria responsible for aniline degradation	1328:1382	The microbial community analysis revealed that the growth of Terrisporobacter, Pseudomonas, and many other bacteria responsible for aniline degradation was inhibited by Mn2+, and so were the strains involved in nitrification.
31726594	1	56	theme	formation	294:302	arg1	terms					281:285	terms	281:285	terms of AGS formation and pollutant removal efficiency	281:335	In this study, the effect of high concentration of Mn2+ on the aerobic granular sludge (AGS) systems for aniline wastewater treatment was systematically investigated in terms of AGS formation and pollutant removal efficiency.
31726594	8	57	theme	many	1328:1331	arg1	bacteria					1339:1346	many other bacteria	1328:1346	many other bacteria responsible for aniline degradation	1328:1382	The microbial community analysis revealed that the growth of Terrisporobacter, Pseudomonas, and many other bacteria responsible for aniline degradation was inhibited by Mn2+, and so were the strains involved in nitrification.
31726594	3	58	theme	high	572:575	arg1	concentration					577:589	the high concentration	568:589	the high concentration of Mn2+	568:597	In the presence of Mn2+, the time to granulation was prolonged from 23 d to 30 d due to the toxicity of the high concentration of Mn2+.
31726594	5	59	theme	functional	864:873	arg1	groups					875:880	some functional groups	859:880	some functional groups	859:880	The extracellular polymeric substances of the granules in the two reactors had similar protein compositions, but some functional groups increased with Mn2+.
31726594	3	60	theme	concentration	577:589	arg1	toxicity					556:563	the toxicity	552:563	the toxicity of the high concentration of Mn2+	552:597	In the presence of Mn2+, the time to granulation was prolonged from 23 d to 30 d due to the toxicity of the high concentration of Mn2+.
31726594	5	61	contain	had	821:823	arg1	substances					774:783	The extracellular polymeric substances	746:783	The extracellular polymeric substances of the granules in the two reactors	746:819	The extracellular polymeric substances of the granules in the two reactors had similar protein compositions, but some functional groups increased with Mn2+.
31726594	5	61	contain	had	821:823	arg2	compositions					841:852	similar protein compositions	825:852	similar protein compositions	825:852	The extracellular polymeric substances of the granules in the two reactors had similar protein compositions, but some functional groups increased with Mn2+.
31726594	6	62	theme	average	1014:1020	arg1	concentrations					1022:1035	average concentrations	1014:1035	average concentrations below 40, 1.0, and 19 mg L-1, respectively	1014:1078	The reactors showed high overall removal efficiency of chemical oxygen demand, NH4+-N, and total nitrogen with average concentrations below 40, 1.0, and 19 mg L-1, respectively, in the effluents.
31726594	1	63	theme	AGS	200:202	arg1	systems					205:211	the aerobic granular sludge (AGS) systems	171:211	the aerobic granular sludge (AGS) systems for aniline wastewater treatment	171:244	In this study, the effect of high concentration of Mn2+ on the aerobic granular sludge (AGS) systems for aniline wastewater treatment was systematically investigated in terms of AGS formation and pollutant removal efficiency.
31726594	0	64	theme	granular	62:69	arg1	sludge					71:76	aerobic granular sludge	54:76	aerobic granular sludge for aniline wastewater treatment	54:109	High concentration of Mn2+ has multiple influences on aerobic granular sludge for aniline wastewater treatment.
31726594	6	65	theme	chemical	958:965	arg1	demand					974:979	chemical oxygen demand	958:979	chemical oxygen demand	958:979	The reactors showed high overall removal efficiency of chemical oxygen demand, NH4+-N, and total nitrogen with average concentrations below 40, 1.0, and 19 mg L-1, respectively, in the effluents.
31726594	8	66	theme	bacteria	1339:1346	arg1	growth					1283:1288	the growth	1279:1288	the growth of Terrisporobacter, Pseudomonas, and many other bacteria responsible for aniline degradation	1279:1382	The microbial community analysis revealed that the growth of Terrisporobacter, Pseudomonas, and many other bacteria responsible for aniline degradation was inhibited by Mn2+, and so were the strains involved in nitrification.
31726594	2	67	theme	sequencing	351:360	arg1	reactors					368:375	Two parallel sequencing batch reactors	338:375	Two parallel sequencing batch reactors	338:375	Two parallel sequencing batch reactors were operated to treat the aniline-rich wastewater with and without 20 mg L-1 of Mn2+.
31726594	3	68	from	30 d	540:543	arg1	prolonged					517:525	prolonged	517:525	prolonged	517:525	In the presence of Mn2+, the time to granulation was prolonged from 23 d to 30 d due to the toxicity of the high concentration of Mn2+.
31726594	3	68	from	30 d	540:543	arg1	time					493:496	the time	489:496	the time to granulation	489:511	In the presence of Mn2+, the time to granulation was prolonged from 23 d to 30 d due to the toxicity of the high concentration of Mn2+.
31726594	6	69	theme	removal	936:942	arg1	efficiency					944:953	high overall removal efficiency	923:953	high overall removal efficiency of chemical oxygen demand, NH4+-N, and total nitrogen	923:1007	The reactors showed high overall removal efficiency of chemical oxygen demand, NH4+-N, and total nitrogen with average concentrations below 40, 1.0, and 19 mg L-1, respectively, in the effluents.
31726594	0	70	theme	aerobic	54:60	arg1	sludge					71:76	aerobic granular sludge	54:76	aerobic granular sludge for aniline wastewater treatment	54:109	High concentration of Mn2+ has multiple influences on aerobic granular sludge for aniline wastewater treatment.
31726594	8	71	theme	other	1333:1337	arg1	bacteria					1339:1346	many other bacteria	1328:1346	many other bacteria responsible for aniline degradation	1328:1382	The microbial community analysis revealed that the growth of Terrisporobacter, Pseudomonas, and many other bacteria responsible for aniline degradation was inhibited by Mn2+, and so were the strains involved in nitrification.
31726594	2	72	theme	parallel	342:349	arg1	reactors					368:375	Two parallel sequencing batch reactors	338:375	Two parallel sequencing batch reactors	338:375	Two parallel sequencing batch reactors were operated to treat the aniline-rich wastewater with and without 20 mg L-1 of Mn2+.
31726594	6	73	theme	overall	928:934	arg1	efficiency					944:953	high overall removal efficiency	923:953	high overall removal efficiency of chemical oxygen demand, NH4+-N, and total nitrogen	923:1007	The reactors showed high overall removal efficiency of chemical oxygen demand, NH4+-N, and total nitrogen with average concentrations below 40, 1.0, and 19 mg L-1, respectively, in the effluents.
29997594	4	0	theme	pasteurized	507:517	arg1	alternative					570:580	the best available alternative	551:580	the best available alternative due to its similarity to the former	551:616	In the absence of their mother's own milk (MOM), pasteurized donor human milk (DHM) could be the best available alternative due to its similarity to the former.
29997594	4	0	theme	pasteurized	507:517	arg1	DHM					537:539	DHM	537:539	DHM	537:539	In the absence of their mother's own milk (MOM), pasteurized donor human milk (DHM) could be the best available alternative due to its similarity to the former.
29997594	4	0	theme	pasteurized	507:517	arg1	milk					531:534	pasteurized donor human milk	507:534	pasteurized donor human milk (DHM)	507:540	In the absence of their mother's own milk (MOM), pasteurized donor human milk (DHM) could be the best available alternative due to its similarity to the former.
29997594	12	1	theme	preterm	1660:1666	arg1	DHM					1672:1674	preterm fed DHM	1660:1674	preterm fed DHM	1660:1674	Preterm infants fed MOM showed a significant greater presence of Bifidobacteriaceae and lower of Staphylococcaceae, Clostridiaceae, and Pasteurellaceae compared to preterm fed DHM.
29997594	15	2	from	enrichment	1970:1979	arg1	pathway					2023:2029	the Glycan biosynthesis and metabolism pathway	1984:2029	the Glycan biosynthesis and metabolism pathway that was not identified in the DHM or in the formula fed groups	1984:2093	Inferred metagenomic analyses showed higher presence of Bifidobacterium genus in mother's milk group was related to enrichment in the Glycan biosynthesis and metabolism pathway that was not identified in the DHM or in the formula fed groups.
29997594	4	3	theme	human	525:529	arg1	alternative					570:580	the best available alternative	551:580	the best available alternative due to its similarity to the former	551:616	In the absence of their mother's own milk (MOM), pasteurized donor human milk (DHM) could be the best available alternative due to its similarity to the former.
29997594	4	3	theme	human	525:529	arg1	DHM					537:539	DHM	537:539	DHM	537:539	In the absence of their mother's own milk (MOM), pasteurized donor human milk (DHM) could be the best available alternative due to its similarity to the former.
29997594	4	3	theme	human	525:529	arg1	milk					531:534	pasteurized donor human milk	507:534	pasteurized donor human milk (DHM)	507:540	In the absence of their mother's own milk (MOM), pasteurized donor human milk (DHM) could be the best available alternative due to its similarity to the former.
29997594	13	4	theme	fed	1685:1687	arg1	profile					1699:1705	Formula fed microbial profile	1677:1705	Formula fed microbial profile	1677:1705	Formula fed microbial profile was different to those observed in preterm fed MOM.
29997594	6	5	theme	preterm	785:791	arg1	microbiota					797:806	preterm gut microbiota	785:806	preterm gut microbiota admitted in a referral neonatal intensive care unit (NICU)	785:865	Our objective was to determine the impact of DHM upon preterm gut microbiota admitted in a referral neonatal intensive care unit (NICU).
29997594	17	6	theme	immune	2307:2312	arg1	system					2314:2319	immune system	2307:2319	immune system	2307:2319	This may have potential beneficial long-term effects on intestinal functionality, immune system, and metabolic activities.
29997594	11	7	theme	preterm	1429:1435	arg1	composition					1448:1458	the preterm microbiota composition	1425:1458	the preterm microbiota composition	1425:1458	Despite the higher variability, no differences in microbial diversity and richness were found, although feeding type significantly influenced the preterm microbiota composition and predictive functional profiles.
29997594	5	8	theme	biological	706:715	arg1	implications					717:728	potential biological implications	696:728	potential biological implications	696:728	However, little is known about the effect of DHM upon preterm microbiota and potential biological implications.
29997594	14	9	theme	microbial	1809:1817	arg1	profiles					1819:1826	closer microbial profiles	1802:1826	closer microbial profiles	1802:1826	Remarkably, preterm infants fed DHM showed closer microbial profiles to preterm fed their MOM.
29997594	8	10	theme	feeding	1057:1063	arg1	practices					1065:1073	feeding practices	1057:1073	feeding practices consisting in their MOM, DHM, or formula	1057:1114	Neonates were classified in three groups according to feeding practices consisting in their MOM, DHM, or formula.
29997594	6	11	theme	DHM	776:778	arg1	impact					766:771	the impact	762:771	the impact of DHM upon preterm gut microbiota admitted in a referral neonatal intensive care unit (NICU)	762:865	Our objective was to determine the impact of DHM upon preterm gut microbiota admitted in a referral neonatal intensive care unit (NICU).
29997594	10	12	theme	rRNA	1261:1264	arg1	sequencing					1271:1280	rRNA gene sequencing	1261:1280	rRNA gene sequencing	1261:1280	Gut microbiota composition was analyzed by 16S rRNA gene sequencing.
29997594	12	13	theme	Preterm	1496:1502	arg1	infants					1504:1510	Preterm infants	1496:1510	Preterm infants fed MOM	1496:1518	Preterm infants fed MOM showed a significant greater presence of Bifidobacteriaceae and lower of Staphylococcaceae, Clostridiaceae, and Pasteurellaceae compared to preterm fed DHM.
29997594	15	14	dep	showed	1884:1889	arg1	related					1959:1965	related	1959:1965	related	1959:1965	Inferred metagenomic analyses showed higher presence of Bifidobacterium genus in mother's milk group was related to enrichment in the Glycan biosynthesis and metabolism pathway that was not identified in the DHM or in the formula fed groups.
29997594	14	15	theme	preterm	1771:1777	arg1	infants					1779:1785	preterm infants	1771:1785	preterm infants fed DHM	1771:1793	Remarkably, preterm infants fed DHM showed closer microbial profiles to preterm fed their MOM.
29997594	6	16	theme	intensive	840:848	arg1	NICU					861:864	NICU	861:864	NICU	861:864	Our objective was to determine the impact of DHM upon preterm gut microbiota admitted in a referral neonatal intensive care unit (NICU).
29997594	6	16	theme	intensive	840:848	arg1	unit					855:858	a referral neonatal intensive care unit	820:858	a referral neonatal intensive care unit (NICU)	820:865	Our objective was to determine the impact of DHM upon preterm gut microbiota admitted in a referral neonatal intensive care unit (NICU).
29997594	7	17	dep	weight	972:977	arg1	g					986:986	≤1,500 g	979:986	a birth weight ≤1,500 g	964:986	A prospective observational cohort study in NICU of 69 neonates <32 weeks of gestation and with a birth weight ≤1,500 g was conducted.
29997594	7	18	theme	cohort	896:901	arg1	study					903:907	A prospective observational cohort study	868:907	A prospective observational cohort study in NICU of 69 neonates <32 weeks of gestation and with a birth weight ≤1,500 g	868:986	A prospective observational cohort study in NICU of 69 neonates <32 weeks of gestation and with a birth weight ≤1,500 g was conducted.
29997594	6	19	theme	referral	822:829	arg1	NICU					861:864	NICU	861:864	NICU	861:864	Our objective was to determine the impact of DHM upon preterm gut microbiota admitted in a referral neonatal intensive care unit (NICU).
29997594	6	19	theme	referral	822:829	arg1	unit					855:858	a referral neonatal intensive care unit	820:858	a referral neonatal intensive care unit (NICU)	820:865	Our objective was to determine the impact of DHM upon preterm gut microbiota admitted in a referral neonatal intensive care unit (NICU).
29997594	7	20	theme	prospective	870:880	arg1	study					903:907	A prospective observational cohort study	868:907	A prospective observational cohort study in NICU of 69 neonates <32 weeks of gestation and with a birth weight ≤1,500 g	868:986	A prospective observational cohort study in NICU of 69 neonates <32 weeks of gestation and with a birth weight ≤1,500 g was conducted.
29997594	11	21	theme	microbial	1333:1341	arg1	diversity					1343:1351	microbial diversity	1333:1351	microbial diversity	1333:1351	Despite the higher variability, no differences in microbial diversity and richness were found, although feeding type significantly influenced the preterm microbiota composition and predictive functional profiles.
29997594	17	22	theme	potential	2239:2247	arg1	effects					2270:2276	potential beneficial long-term effects	2239:2276	potential beneficial long-term effects	2239:2276	This may have potential beneficial long-term effects on intestinal functionality, immune system, and metabolic activities.
29997594	15	23	theme	Inferred	1854:1861	arg1	analyses					1875:1882	Inferred metagenomic analyses	1854:1882	Inferred metagenomic analyses	1854:1882	Inferred metagenomic analyses showed higher presence of Bifidobacterium genus in mother's milk group was related to enrichment in the Glycan biosynthesis and metabolism pathway that was not identified in the DHM or in the formula fed groups.
29997594	0	24	theme	Donor	66:70	arg1	Milk					78:81	Donor Human Milk	66:81	Donor Human Milk	66:81	Preterm Gut Microbiome Depending on Feeding Type: Significance of Donor Human Milk.
29997594	9	25	theme	full	1151:1154	arg1	feeding					1164:1170	full enteral feeding	1151:1170	full enteral feeding (defined as ≥150 cc/kg/day)	1151:1198	Fecal samples were collected when full enteral feeding (defined as ≥150 cc/kg/day) was achieved.
29997594	1	26	theme	antibiotic	147:156	arg1	age					142:144	gestational age	130:144	gestational age	130:144	Preterm microbial colonization is affected by gestational age, antibiotic treatment, type of birth, but also by type of feeding.
29997594	1	26	theme	antibiotic	147:156	arg1	treatment					158:166	antibiotic treatment	147:166	antibiotic treatment	147:166	Preterm microbial colonization is affected by gestational age, antibiotic treatment, type of birth, but also by type of feeding.
29997594	0	27	theme	Milk	78:81	arg1	Significance					50:61	Feeding Type: Significance	36:61	Feeding Type: Significance of Donor Human Milk	36:81	Preterm Gut Microbiome Depending on Feeding Type: Significance of Donor Human Milk.
29997594	7	28	dep	prospective	870:880	arg1	observational					882:894	observational	882:894	observational	882:894	A prospective observational cohort study in NICU of 69 neonates <32 weeks of gestation and with a birth weight ≤1,500 g was conducted.
29997594	12	29	theme	greater	1541:1547	arg1	presence					1549:1556	a significant greater presence	1527:1556	a significant greater presence of Bifidobacteriaceae and lower of Staphylococcaceae, Clostridiaceae, and Pasteurellaceae	1527:1646	Preterm infants fed MOM showed a significant greater presence of Bifidobacteriaceae and lower of Staphylococcaceae, Clostridiaceae, and Pasteurellaceae compared to preterm fed DHM.
29997594	7	30	with	NICU	912:915	arg1	weight					972:977	a birth weight	964:977	a birth weight ≤1,500 g	964:986	A prospective observational cohort study in NICU of 69 neonates <32 weeks of gestation and with a birth weight ≤1,500 g was conducted.
29997594	9	31	dep	feeding	1164:1170	arg1	defined					1173:1179	defined	1173:1179	defined as ≥150 cc/kg/day	1173:1197	Fecal samples were collected when full enteral feeding (defined as ≥150 cc/kg/day) was achieved.
29997594	11	32	theme	higher	1295:1300	arg1	variability					1302:1312	the higher variability	1291:1312	the higher variability	1291:1312	Despite the higher variability, no differences in microbial diversity and richness were found, although feeding type significantly influenced the preterm microbiota composition and predictive functional profiles.
29997594	3	33	theme	reduced	393:399	arg1	risk					401:404	a reduced risk	391:404	a reduced risk of necrotizing enterocolitis and late onset sepsis	391:455	In preterm infants breast milk has been associated with improved growth and cognitive development and a reduced risk of necrotizing enterocolitis and late onset sepsis.
29997594	17	34	theme	beneficial	2249:2258	arg1	effects					2270:2276	potential beneficial long-term effects	2239:2276	potential beneficial long-term effects	2239:2276	This may have potential beneficial long-term effects on intestinal functionality, immune system, and metabolic activities.
29997594	13	35	theme	microbial	1689:1697	arg1	profile					1699:1705	Formula fed microbial profile	1677:1705	Formula fed microbial profile	1677:1705	Formula fed microbial profile was different to those observed in preterm fed MOM.
29997594	13	36	located	observed	1730:1737	arg2	those					1724:1728	those	1724:1728	those	1724:1728	Formula fed microbial profile was different to those observed in preterm fed MOM.
29997594	13	36	located	observed	1730:1737	arg1	MOM					1754:1756	preterm fed MOM	1742:1756	preterm fed MOM	1742:1756	Formula fed microbial profile was different to those observed in preterm fed MOM.
29997594	0	37	theme	Preterm	0:6	arg1	Microbiome					12:21	Preterm Gut Microbiome	0:21	Preterm Gut Microbiome	0:21	Preterm Gut Microbiome Depending on Feeding Type: Significance of Donor Human Milk.
29997594	4	38	theme	milk	495:498	arg1	alternative					570:580	the best available alternative	551:580	the best available alternative due to its similarity to the former	551:616	In the absence of their mother's own milk (MOM), pasteurized donor human milk (DHM) could be the best available alternative due to its similarity to the former.
29997594	4	38	theme	milk	495:498	arg1	milk					531:534	pasteurized donor human milk	507:534	pasteurized donor human milk (DHM)	507:540	In the absence of their mother's own milk (MOM), pasteurized donor human milk (DHM) could be the best available alternative due to its similarity to the former.
29997594	15	39	theme	genus	1926:1930	arg1	presence					1898:1905	higher presence	1891:1905	higher presence of Bifidobacterium genus in mother's milk group	1891:1953	Inferred metagenomic analyses showed higher presence of Bifidobacterium genus in mother's milk group was related to enrichment in the Glycan biosynthesis and metabolism pathway that was not identified in the DHM or in the formula fed groups.
29997594	3	40	theme	enterocolitis	421:433	arg1	risk					401:404	a reduced risk	391:404	a reduced risk of necrotizing enterocolitis and late onset sepsis	391:455	In preterm infants breast milk has been associated with improved growth and cognitive development and a reduced risk of necrotizing enterocolitis and late onset sepsis.
29997594	3	40	theme	enterocolitis	421:433	arg1	growth					354:359	improved growth	345:359	improved growth	345:359	In preterm infants breast milk has been associated with improved growth and cognitive development and a reduced risk of necrotizing enterocolitis and late onset sepsis.
29997594	3	40	theme	enterocolitis	421:433	arg1	development					375:385	cognitive development	365:385	cognitive development	365:385	In preterm infants breast milk has been associated with improved growth and cognitive development and a reduced risk of necrotizing enterocolitis and late onset sepsis.
29997594	1	41	theme	microbial	92:100	arg1	colonization					102:113	Preterm microbial colonization	84:113	Preterm microbial colonization	84:113	Preterm microbial colonization is affected by gestational age, antibiotic treatment, type of birth, but also by type of feeding.
29997594	15	42	theme	fed	2084:2086	arg1	groups					2088:2093	the formula fed groups	2072:2093	the formula fed groups	2072:2093	Inferred metagenomic analyses showed higher presence of Bifidobacterium genus in mother's milk group was related to enrichment in the Glycan biosynthesis and metabolism pathway that was not identified in the DHM or in the formula fed groups.
29997594	3	43	theme	cognitive	365:373	arg1	development					375:385	cognitive development	365:385	cognitive development	365:385	In preterm infants breast milk has been associated with improved growth and cognitive development and a reduced risk of necrotizing enterocolitis and late onset sepsis.
29997594	17	44	theme	intestinal	2281:2290	arg1	functionality					2292:2304	intestinal functionality	2281:2304	intestinal functionality	2281:2304	This may have potential beneficial long-term effects on intestinal functionality, immune system, and metabolic activities.
29997594	13	45	theme	fed	1750:1752	arg1	MOM					1754:1756	preterm fed MOM	1742:1756	preterm fed MOM	1742:1756	Formula fed microbial profile was different to those observed in preterm fed MOM.
29997594	5	46	theme	preterm	673:679	arg1	microbiota					681:690	preterm microbiota	673:690	preterm microbiota	673:690	However, little is known about the effect of DHM upon preterm microbiota and potential biological implications.
29997594	15	47	dep	biosynthesis	1995:2006	arg1	the					1984:1986	the	1984:1986	the	1984:1986	Inferred metagenomic analyses showed higher presence of Bifidobacterium genus in mother's milk group was related to enrichment in the Glycan biosynthesis and metabolism pathway that was not identified in the DHM or in the formula fed groups.
29997594	15	48	theme	higher	1891:1896	arg1	presence					1898:1905	higher presence	1891:1905	higher presence of Bifidobacterium genus in mother's milk group	1891:1953	Inferred metagenomic analyses showed higher presence of Bifidobacterium genus in mother's milk group was related to enrichment in the Glycan biosynthesis and metabolism pathway that was not identified in the DHM or in the formula fed groups.
29997594	11	49	theme	functional	1475:1484	arg1	profiles					1486:1493	predictive functional profiles	1464:1493	predictive functional profiles	1464:1493	Despite the higher variability, no differences in microbial diversity and richness were found, although feeding type significantly influenced the preterm microbiota composition and predictive functional profiles.
29997594	0	50	theme	Type	44:47	arg1	Significance					50:61	Feeding Type: Significance	36:61	Feeding Type: Significance of Donor Human Milk	36:81	Preterm Gut Microbiome Depending on Feeding Type: Significance of Donor Human Milk.
29997594	11	51	from	differences	1318:1328	arg1	diversity					1343:1351	microbial diversity	1333:1351	microbial diversity	1333:1351	Despite the higher variability, no differences in microbial diversity and richness were found, although feeding type significantly influenced the preterm microbiota composition and predictive functional profiles.
29997594	11	51	from	differences	1318:1328	arg1	richness					1357:1364	richness	1357:1364	richness	1357:1364	Despite the higher variability, no differences in microbial diversity and richness were found, although feeding type significantly influenced the preterm microbiota composition and predictive functional profiles.
29997594	3	52	theme	late	439:442	arg1	onset					444:448	late onset	439:448	late onset	439:448	In preterm infants breast milk has been associated with improved growth and cognitive development and a reduced risk of necrotizing enterocolitis and late onset sepsis.
29997594	15	53	theme	biosynthesis	1995:2006	arg1	pathway					2023:2029	the Glycan biosynthesis and metabolism pathway	1984:2029	the Glycan biosynthesis and metabolism pathway that was not identified in the DHM or in the formula fed groups	1984:2093	Inferred metagenomic analyses showed higher presence of Bifidobacterium genus in mother's milk group was related to enrichment in the Glycan biosynthesis and metabolism pathway that was not identified in the DHM or in the formula fed groups.
29997594	17	54	contain	have	2234:2237	arg1	This					2225:2228	This	2225:2228	This	2225:2228	This may have potential beneficial long-term effects on intestinal functionality, immune system, and metabolic activities.
29997594	17	54	contain	have	2234:2237	arg2	effects					2270:2276	potential beneficial long-term effects	2239:2276	potential beneficial long-term effects	2239:2276	This may have potential beneficial long-term effects on intestinal functionality, immune system, and metabolic activities.
29997594	10	55	theme	Gut	1214:1216	arg1	composition					1229:1239	Gut microbiota composition	1214:1239	Gut microbiota composition	1214:1239	Gut microbiota composition was analyzed by 16S rRNA gene sequencing.
29997594	12	56	theme	fed	1668:1670	arg1	DHM					1672:1674	preterm fed DHM	1660:1674	preterm fed DHM	1660:1674	Preterm infants fed MOM showed a significant greater presence of Bifidobacteriaceae and lower of Staphylococcaceae, Clostridiaceae, and Pasteurellaceae compared to preterm fed DHM.
29997594	13	57	theme	Formula	1677:1683	arg1	profile					1699:1705	Formula fed microbial profile	1677:1705	Formula fed microbial profile	1677:1705	Formula fed microbial profile was different to those observed in preterm fed MOM.
29997594	11	58	theme	microbiota	1437:1446	arg1	composition					1448:1458	the preterm microbiota composition	1425:1458	the preterm microbiota composition	1425:1458	Despite the higher variability, no differences in microbial diversity and richness were found, although feeding type significantly influenced the preterm microbiota composition and predictive functional profiles.
29997594	6	59	theme	gut	793:795	arg1	microbiota					797:806	preterm gut microbiota	785:806	preterm gut microbiota admitted in a referral neonatal intensive care unit (NICU)	785:865	Our objective was to determine the impact of DHM upon preterm gut microbiota admitted in a referral neonatal intensive care unit (NICU).
29997594	12	60	dep	showed	1520:1525	arg1	compared					1648:1655	compared	1648:1655	showed a significant greater presence of Bifidobacteriaceae and lower of Staphylococcaceae, Clostridiaceae, and Pasteurellaceae compared to preterm fed DHM	1520:1674	Preterm infants fed MOM showed a significant greater presence of Bifidobacteriaceae and lower of Staphylococcaceae, Clostridiaceae, and Pasteurellaceae compared to preterm fed DHM.
29997594	4	61	theme	donor	519:523	arg1	alternative					570:580	the best available alternative	551:580	the best available alternative due to its similarity to the former	551:616	In the absence of their mother's own milk (MOM), pasteurized donor human milk (DHM) could be the best available alternative due to its similarity to the former.
29997594	4	61	theme	donor	519:523	arg1	DHM					537:539	DHM	537:539	DHM	537:539	In the absence of their mother's own milk (MOM), pasteurized donor human milk (DHM) could be the best available alternative due to its similarity to the former.
29997594	4	61	theme	donor	519:523	arg1	milk					531:534	pasteurized donor human milk	507:534	pasteurized donor human milk (DHM)	507:540	In the absence of their mother's own milk (MOM), pasteurized donor human milk (DHM) could be the best available alternative due to its similarity to the former.
29997594	7	62	dep	neonates	923:930	arg1	weeks					936:940	<32 weeks	932:940	69 neonates <32 weeks of gestation	920:953	A prospective observational cohort study in NICU of 69 neonates <32 weeks of gestation and with a birth weight ≤1,500 g was conducted.
29997594	7	63	theme	birth	966:970	arg1	weight					972:977	a birth weight	964:977	a birth weight ≤1,500 g	964:986	A prospective observational cohort study in NICU of 69 neonates <32 weeks of gestation and with a birth weight ≤1,500 g was conducted.
29997594	11	64	theme	feeding	1387:1393	arg1	type					1395:1398	feeding type	1387:1398	feeding type	1387:1398	Despite the higher variability, no differences in microbial diversity and richness were found, although feeding type significantly influenced the preterm microbiota composition and predictive functional profiles.
29997594	12	65	theme	Pasteurellaceae	1632:1646	arg1	presence					1549:1556	a significant greater presence	1527:1556	a significant greater presence of Bifidobacteriaceae and lower of Staphylococcaceae, Clostridiaceae, and Pasteurellaceae	1527:1646	Preterm infants fed MOM showed a significant greater presence of Bifidobacteriaceae and lower of Staphylococcaceae, Clostridiaceae, and Pasteurellaceae compared to preterm fed DHM.
29997594	17	66	theme	metabolic	2326:2334	arg1	activities					2336:2345	metabolic activities	2326:2345	metabolic activities	2326:2345	This may have potential beneficial long-term effects on intestinal functionality, immune system, and metabolic activities.
29997594	4	67	theme	available	560:568	arg1	alternative					570:580	the best available alternative	551:580	the best available alternative due to its similarity to the former	551:616	In the absence of their mother's own milk (MOM), pasteurized donor human milk (DHM) could be the best available alternative due to its similarity to the former.
29997594	4	67	theme	available	560:568	arg1	milk					531:534	pasteurized donor human milk	507:534	pasteurized donor human milk (DHM)	507:540	In the absence of their mother's own milk (MOM), pasteurized donor human milk (DHM) could be the best available alternative due to its similarity to the former.
29997594	5	68	theme	DHM	664:666	arg1	effect					654:659	the effect	650:659	the effect of DHM upon preterm microbiota and potential biological implications	650:728	However, little is known about the effect of DHM upon preterm microbiota and potential biological implications.
29997594	10	69	theme	gene	1266:1269	arg1	sequencing					1271:1280	rRNA gene sequencing	1261:1280	rRNA gene sequencing	1261:1280	Gut microbiota composition was analyzed by 16S rRNA gene sequencing.
29997594	2	70	theme	human	272:276	arg1	nutrition					278:286	human nutrition	272:286	human nutrition	272:286	Breast milk has been acknowledged as the gold standard for human nutrition.
29997594	14	71	theme	closer	1802:1807	arg1	profiles					1819:1826	closer microbial profiles	1802:1826	closer microbial profiles	1802:1826	Remarkably, preterm infants fed DHM showed closer microbial profiles to preterm fed their MOM.
29997594	7	72	theme	gestation	945:953	arg1	weeks					936:940	<32 weeks	932:940	69 neonates <32 weeks of gestation	920:953	A prospective observational cohort study in NICU of 69 neonates <32 weeks of gestation and with a birth weight ≤1,500 g was conducted.
29997594	15	73	theme	metabolism	2012:2021	arg1	pathway					2023:2029	the Glycan biosynthesis and metabolism pathway	1984:2029	the Glycan biosynthesis and metabolism pathway that was not identified in the DHM or in the formula fed groups	1984:2093	Inferred metagenomic analyses showed higher presence of Bifidobacterium genus in mother's milk group was related to enrichment in the Glycan biosynthesis and metabolism pathway that was not identified in the DHM or in the formula fed groups.
29997594	1	74	theme	birth	177:181	arg1	type					169:172	type	169:172	type of birth	169:181	Preterm microbial colonization is affected by gestational age, antibiotic treatment, type of birth, but also by type of feeding.
29997594	1	74	theme	birth	177:181	arg1	age					142:144	gestational age	130:144	gestational age	130:144	Preterm microbial colonization is affected by gestational age, antibiotic treatment, type of birth, but also by type of feeding.
29997594	15	75	theme	formula	2076:2082	arg1	groups					2088:2093	the formula fed groups	2072:2093	the formula fed groups	2072:2093	Inferred metagenomic analyses showed higher presence of Bifidobacterium genus in mother's milk group was related to enrichment in the Glycan biosynthesis and metabolism pathway that was not identified in the DHM or in the formula fed groups.
29997594	7	76	from	study	903:907	arg1	NICU					912:915	NICU	912:915	NICU of 69 neonates <32 weeks of gestation and with a birth weight ≤1,500 g	912:986	A prospective observational cohort study in NICU of 69 neonates <32 weeks of gestation and with a birth weight ≤1,500 g was conducted.
29997594	16	77	theme	similar	2152:2158	arg1	microbiome					2136:2145	an intestinal microbiome	2122:2145	an intestinal microbiome more similar to MOM	2122:2165	In conclusion, DHM favors an intestinal microbiome more similar to MOM than formula despite the differences between MOM and DHM.
29997594	14	78	dep	preterm	1831:1837	arg1	fed					1839:1841	fed	1839:1841	to preterm fed their MOM	1828:1851	Remarkably, preterm infants fed DHM showed closer microbial profiles to preterm fed their MOM.
29997594	6	79	theme	care	850:853	arg1	NICU					861:864	NICU	861:864	NICU	861:864	Our objective was to determine the impact of DHM upon preterm gut microbiota admitted in a referral neonatal intensive care unit (NICU).
29997594	6	79	theme	care	850:853	arg1	unit					855:858	a referral neonatal intensive care unit	820:858	a referral neonatal intensive care unit (NICU)	820:865	Our objective was to determine the impact of DHM upon preterm gut microbiota admitted in a referral neonatal intensive care unit (NICU).
29997594	7	80	theme	neonates	923:930	arg1	NICU					912:915	NICU	912:915	NICU of 69 neonates <32 weeks of gestation and with a birth weight ≤1,500 g	912:986	A prospective observational cohort study in NICU of 69 neonates <32 weeks of gestation and with a birth weight ≤1,500 g was conducted.
29997594	6	81	theme	neonatal	831:838	arg1	NICU					861:864	NICU	861:864	NICU	861:864	Our objective was to determine the impact of DHM upon preterm gut microbiota admitted in a referral neonatal intensive care unit (NICU).
29997594	6	81	theme	neonatal	831:838	arg1	unit					855:858	a referral neonatal intensive care unit	820:858	a referral neonatal intensive care unit (NICU)	820:865	Our objective was to determine the impact of DHM upon preterm gut microbiota admitted in a referral neonatal intensive care unit (NICU).
29997594	9	82	theme	Fecal	1117:1121	arg1	samples					1123:1129	Fecal samples	1117:1129	Fecal samples	1117:1129	Fecal samples were collected when full enteral feeding (defined as ≥150 cc/kg/day) was achieved.
29997594	12	83	theme	Staphylococcaceae	1593:1609	arg1	presence					1549:1556	a significant greater presence	1527:1556	a significant greater presence of Bifidobacteriaceae and lower of Staphylococcaceae, Clostridiaceae, and Pasteurellaceae	1527:1646	Preterm infants fed MOM showed a significant greater presence of Bifidobacteriaceae and lower of Staphylococcaceae, Clostridiaceae, and Pasteurellaceae compared to preterm fed DHM.
29997594	2	84	theme	Breast	213:218	arg1	standard					259:266	standard	259:266	standard	259:266	Breast milk has been acknowledged as the gold standard for human nutrition.
29997594	2	84	theme	Breast	213:218	arg1	milk					220:223	Breast milk	213:223	Breast milk	213:223	Breast milk has been acknowledged as the gold standard for human nutrition.
29997594	1	85	theme	gestational	130:140	arg1	type					169:172	type	169:172	type of birth	169:181	Preterm microbial colonization is affected by gestational age, antibiotic treatment, type of birth, but also by type of feeding.
29997594	1	85	theme	gestational	130:140	arg1	age					142:144	gestational age	130:144	gestational age	130:144	Preterm microbial colonization is affected by gestational age, antibiotic treatment, type of birth, but also by type of feeding.
29997594	1	85	theme	gestational	130:140	arg1	treatment					158:166	antibiotic treatment	147:166	antibiotic treatment	147:166	Preterm microbial colonization is affected by gestational age, antibiotic treatment, type of birth, but also by type of feeding.
29997594	12	86	theme	Clostridiaceae	1612:1625	arg1	presence					1549:1556	a significant greater presence	1527:1556	a significant greater presence of Bifidobacteriaceae and lower of Staphylococcaceae, Clostridiaceae, and Pasteurellaceae	1527:1646	Preterm infants fed MOM showed a significant greater presence of Bifidobacteriaceae and lower of Staphylococcaceae, Clostridiaceae, and Pasteurellaceae compared to preterm fed DHM.
29997594	3	87	theme	preterm	292:298	arg1	infants					300:306	preterm infants	292:306	preterm infants	292:306	In preterm infants breast milk has been associated with improved growth and cognitive development and a reduced risk of necrotizing enterocolitis and late onset sepsis.
29997594	15	88	from	presence	1898:1905	arg1	group					1949:1953	mother's milk group	1935:1953	mother's milk group	1935:1953	Inferred metagenomic analyses showed higher presence of Bifidobacterium genus in mother's milk group was related to enrichment in the Glycan biosynthesis and metabolism pathway that was not identified in the DHM or in the formula fed groups.
29997594	0	89	theme	Human	72:76	arg1	Milk					78:81	Donor Human Milk	66:81	Donor Human Milk	66:81	Preterm Gut Microbiome Depending on Feeding Type: Significance of Donor Human Milk.
29997594	12	90	theme	Bifidobacteriaceae	1561:1578	arg1	presence					1549:1556	a significant greater presence	1527:1556	a significant greater presence of Bifidobacteriaceae and lower of Staphylococcaceae, Clostridiaceae, and Pasteurellaceae	1527:1646	Preterm infants fed MOM showed a significant greater presence of Bifidobacteriaceae and lower of Staphylococcaceae, Clostridiaceae, and Pasteurellaceae compared to preterm fed DHM.
29997594	15	91	theme	metagenomic	1863:1873	arg1	analyses					1875:1882	Inferred metagenomic analyses	1854:1882	Inferred metagenomic analyses	1854:1882	Inferred metagenomic analyses showed higher presence of Bifidobacterium genus in mother's milk group was related to enrichment in the Glycan biosynthesis and metabolism pathway that was not identified in the DHM or in the formula fed groups.
29997594	9	92	theme	enteral	1156:1162	arg1	feeding					1164:1170	full enteral feeding	1151:1170	full enteral feeding (defined as ≥150 cc/kg/day)	1151:1198	Fecal samples were collected when full enteral feeding (defined as ≥150 cc/kg/day) was achieved.
29997594	12	93	theme	lower	1584:1588	arg1	presence					1549:1556	a significant greater presence	1527:1556	a significant greater presence of Bifidobacteriaceae and lower of Staphylococcaceae, Clostridiaceae, and Pasteurellaceae	1527:1646	Preterm infants fed MOM showed a significant greater presence of Bifidobacteriaceae and lower of Staphylococcaceae, Clostridiaceae, and Pasteurellaceae compared to preterm fed DHM.
29997594	15	94	attach	presence	1898:1905	arg1	group					1949:1953	mother's milk group	1935:1953	mother's milk group	1935:1953	Inferred metagenomic analyses showed higher presence of Bifidobacterium genus in mother's milk group was related to enrichment in the Glycan biosynthesis and metabolism pathway that was not identified in the DHM or in the formula fed groups.
29997594	15	94	attach	presence	1898:1905	arg2	genus					1926:1930	Bifidobacterium genus	1910:1930	Bifidobacterium genus	1910:1930	Inferred metagenomic analyses showed higher presence of Bifidobacterium genus in mother's milk group was related to enrichment in the Glycan biosynthesis and metabolism pathway that was not identified in the DHM or in the formula fed groups.
29997594	12	95	theme	significant	1529:1539	arg1	presence					1549:1556	a significant greater presence	1527:1556	a significant greater presence of Bifidobacteriaceae and lower of Staphylococcaceae, Clostridiaceae, and Pasteurellaceae	1527:1646	Preterm infants fed MOM showed a significant greater presence of Bifidobacteriaceae and lower of Staphylococcaceae, Clostridiaceae, and Pasteurellaceae compared to preterm fed DHM.
29997594	3	96	theme	breast	308:313	arg1	milk					315:318	breast milk	308:318	breast milk	308:318	In preterm infants breast milk has been associated with improved growth and cognitive development and a reduced risk of necrotizing enterocolitis and late onset sepsis.
29997594	16	97	theme	intestinal	2125:2134	arg1	microbiome					2136:2145	an intestinal microbiome	2122:2145	an intestinal microbiome more similar to MOM	2122:2165	In conclusion, DHM favors an intestinal microbiome more similar to MOM than formula despite the differences between MOM and DHM.
29997594	0	98	theme	Gut	8:10	arg1	Microbiome					12:21	Preterm Gut Microbiome	0:21	Preterm Gut Microbiome	0:21	Preterm Gut Microbiome Depending on Feeding Type: Significance of Donor Human Milk.
29997594	4	99	dep	milk	495:498	arg1	the					461:463	the	461:463	the	461:463	In the absence of their mother's own milk (MOM), pasteurized donor human milk (DHM) could be the best available alternative due to its similarity to the former.
29997594	4	99	dep	milk	495:498	arg1	absence					465:471	absence	465:471	absence	465:471	In the absence of their mother's own milk (MOM), pasteurized donor human milk (DHM) could be the best available alternative due to its similarity to the former.
29997594	15	100	theme	milk	1944:1947	arg1	group					1949:1953	mother's milk group	1935:1953	mother's milk group	1935:1953	Inferred metagenomic analyses showed higher presence of Bifidobacterium genus in mother's milk group was related to enrichment in the Glycan biosynthesis and metabolism pathway that was not identified in the DHM or in the formula fed groups.
29997594	3	101	theme	improved	345:352	arg1	growth					354:359	improved growth	345:359	improved growth	345:359	In preterm infants breast milk has been associated with improved growth and cognitive development and a reduced risk of necrotizing enterocolitis and late onset sepsis.
29997594	17	102	theme	long-term	2260:2268	arg1	effects					2270:2276	potential beneficial long-term effects	2239:2276	potential beneficial long-term effects	2239:2276	This may have potential beneficial long-term effects on intestinal functionality, immune system, and metabolic activities.
29997594	1	103	theme	Preterm	84:90	arg1	colonization					102:113	Preterm microbial colonization	84:113	Preterm microbial colonization	84:113	Preterm microbial colonization is affected by gestational age, antibiotic treatment, type of birth, but also by type of feeding.
29997594	13	104	theme	preterm	1742:1748	arg1	MOM					1754:1756	preterm fed MOM	1742:1756	preterm fed MOM	1742:1756	Formula fed microbial profile was different to those observed in preterm fed MOM.
29997594	0	105	theme	Feeding	36:42	arg1	Significance					50:61	Feeding Type: Significance	36:61	Feeding Type: Significance of Donor Human Milk	36:81	Preterm Gut Microbiome Depending on Feeding Type: Significance of Donor Human Milk.
29997594	15	106	theme	Bifidobacterium	1910:1924	arg1	genus					1926:1930	Bifidobacterium genus	1910:1930	Bifidobacterium genus	1910:1930	Inferred metagenomic analyses showed higher presence of Bifidobacterium genus in mother's milk group was related to enrichment in the Glycan biosynthesis and metabolism pathway that was not identified in the DHM or in the formula fed groups.
29997594	11	107	theme	predictive	1464:1473	arg1	profiles					1486:1493	predictive functional profiles	1464:1493	predictive functional profiles	1464:1493	Despite the higher variability, no differences in microbial diversity and richness were found, although feeding type significantly influenced the preterm microbiota composition and predictive functional profiles.
29997594	3	108	theme	necrotizing	409:419	arg1	enterocolitis					421:433	necrotizing enterocolitis	409:433	necrotizing enterocolitis	409:433	In preterm infants breast milk has been associated with improved growth and cognitive development and a reduced risk of necrotizing enterocolitis and late onset sepsis.
29997594	4	109	theme	own	491:493	arg1	MOM					501:503	MOM	501:503	MOM	501:503	In the absence of their mother's own milk (MOM), pasteurized donor human milk (DHM) could be the best available alternative due to its similarity to the former.
29997594	4	109	theme	own	491:493	arg1	milk					495:498	their mother's own milk	476:498	their mother's own milk (MOM)	476:504	In the absence of their mother's own milk (MOM), pasteurized donor human milk (DHM) could be the best available alternative due to its similarity to the former.
29997594	15	110	theme	Glycan	1988:1993	arg1	biosynthesis					1995:2006	Glycan biosynthesis	1988:2006	Glycan biosynthesis	1988:2006	Inferred metagenomic analyses showed higher presence of Bifidobacterium genus in mother's milk group was related to enrichment in the Glycan biosynthesis and metabolism pathway that was not identified in the DHM or in the formula fed groups.
29997594	10	111	theme	microbiota	1218:1227	arg1	composition					1229:1239	Gut microbiota composition	1214:1239	Gut microbiota composition	1214:1239	Gut microbiota composition was analyzed by 16S rRNA gene sequencing.
29997594	3	112	theme	onset	444:448	arg1	risk					401:404	a reduced risk	391:404	a reduced risk of necrotizing enterocolitis and late onset sepsis	391:455	In preterm infants breast milk has been associated with improved growth and cognitive development and a reduced risk of necrotizing enterocolitis and late onset sepsis.
29997594	3	112	theme	onset	444:448	arg1	growth					354:359	improved growth	345:359	improved growth	345:359	In preterm infants breast milk has been associated with improved growth and cognitive development and a reduced risk of necrotizing enterocolitis and late onset sepsis.
29997594	3	112	theme	onset	444:448	arg1	development					375:385	cognitive development	365:385	cognitive development	365:385	In preterm infants breast milk has been associated with improved growth and cognitive development and a reduced risk of necrotizing enterocolitis and late onset sepsis.
29997594	3	113	dep	enterocolitis	421:433	arg1	sepsis					450:455	sepsis	450:455	sepsis	450:455	In preterm infants breast milk has been associated with improved growth and cognitive development and a reduced risk of necrotizing enterocolitis and late onset sepsis.
29997594	5	114	theme	potential	696:704	arg1	implications					717:728	potential biological implications	696:728	potential biological implications	696:728	However, little is known about the effect of DHM upon preterm microbiota and potential biological implications.
29997594	1	115	theme	feeding	204:210	arg1	type					196:199	type	196:199	type of feeding	196:210	Preterm microbial colonization is affected by gestational age, antibiotic treatment, type of birth, but also by type of feeding.
30227357	4	0	theme	advanced	881:888	arg1	ICP-MS					946:951	ICP-MS	946:951	ICP-MS	946:951	Here, we describe a platform of analytical methods to determine the composition of several metals in different sample matrices using an advanced automated Inductively Coupled Plasma-Mass Spectrometry (ICP-MS).
30227357	4	0	theme	advanced	881:888	arg1	Spectrometry					932:943	an advanced automated Inductively Coupled Plasma-Mass Spectrometry	878:943	an advanced automated Inductively Coupled Plasma-Mass Spectrometry (ICP-MS)	878:952	Here, we describe a platform of analytical methods to determine the composition of several metals in different sample matrices using an advanced automated Inductively Coupled Plasma-Mass Spectrometry (ICP-MS).
30227357	3	1	theme	product	728:734	arg1	quality					736:742	product quality	728:742	product quality	728:742	Because such concentration changes can impact cell growth, manufacturing yield and product quality the alteration/fluctuation in media composition should be well controlled to maintain product quality.
30227357	0	2	theme	monoclonal	146:155	arg1	antibody					157:164	an IgG3:κ monoclonal antibody	136:164	an IgG3:κ monoclonal antibody during production	136:182	An ICP-MS platform for metal content assessment of cell culture media and evaluation of spikes in metal concentration on the quality of an IgG3:κ monoclonal antibody during production.
30227357	2	3	dep	25,000 ppb	531:540	arg1	to					528:529	to	528:529	to	528:529	However, the concentrations of metals in commercially available chemically defined media can range from 1 to 25,000 ppb.
30227357	5	4	dep	applied	1044:1050	arg1	κ					1256:1256	κ	1256:1256	κ produced from a murine-hybridoma cell line in bench-top parallel bioreactors due to a spike in copper and iron concentration	1256:1381	These methods, validated to ICH Q2R1 regulatory validation parameters, were successfully applied to- (a) screen cell culture media; (b) determine changes in the metal concentration during cell growth in spinner flasks, and, (c) determine effect on the glycosylation pattern and homogeneity of an IgG3:κ produced from a murine-hybridoma cell line in bench-top parallel bioreactors due to a spike in copper and iron concentration.
30227357	4	5	theme	sample	856:861	arg1	matrices					863:870	different sample matrices	846:870	different sample matrices	846:870	Here, we describe a platform of analytical methods to determine the composition of several metals in different sample matrices using an advanced automated Inductively Coupled Plasma-Mass Spectrometry (ICP-MS).
30227357	3	6	theme	concentration	556:568	arg1	changes					570:576	such concentration changes	551:576	such concentration changes	551:576	Because such concentration changes can impact cell growth, manufacturing yield and product quality the alteration/fluctuation in media composition should be well controlled to maintain product quality.
30227357	0	7	theme	metal	98:102	arg1	concentration					104:116	metal concentration	98:116	metal concentration	98:116	An ICP-MS platform for metal content assessment of cell culture media and evaluation of spikes in metal concentration on the quality of an IgG3:κ monoclonal antibody during production.
30227357	1	8	theme	therapeutic	357:367	arg1	distribution					408:419	distribution	408:419	distribution	408:419	Metal ions can be enzyme cofactors and can directly influence the kinetics of biochemical reactions that also influence the biological production and quality attributes of therapeutic proteins, such as glycan formation and distribution.
30227357	1	8	theme	therapeutic	357:367	arg1	formation					394:402	glycan formation	387:402	glycan formation	387:402	Metal ions can be enzyme cofactors and can directly influence the kinetics of biochemical reactions that also influence the biological production and quality attributes of therapeutic proteins, such as glycan formation and distribution.
30227357	1	8	theme	therapeutic	357:367	arg1	proteins					369:376	therapeutic proteins	357:376	therapeutic proteins	357:376	Metal ions can be enzyme cofactors and can directly influence the kinetics of biochemical reactions that also influence the biological production and quality attributes of therapeutic proteins, such as glycan formation and distribution.
30227357	6	9	theme	culture	1447:1453	arg1	media					1455:1459	the cell culture media	1438:1459	the cell culture media	1438:1459	Our results show that maintenance of metal content in the cell culture media is critical for product consistency of the IgG3:κ produced.
30227357	0	10	from	media	64:68	arg1	concentration					104:116	metal concentration	98:116	metal concentration	98:116	An ICP-MS platform for metal content assessment of cell culture media and evaluation of spikes in metal concentration on the quality of an IgG3:κ monoclonal antibody during production.
30227357	3	11	theme	cell	589:592	arg1	growth					594:599	cell growth	589:599	cell growth	589:599	Because such concentration changes can impact cell growth, manufacturing yield and product quality the alteration/fluctuation in media composition should be well controlled to maintain product quality.
30227357	1	12	theme	Metal	185:189	arg1	ions					191:194	Metal ions	185:194	Metal ions	185:194	Metal ions can be enzyme cofactors and can directly influence the kinetics of biochemical reactions that also influence the biological production and quality attributes of therapeutic proteins, such as glycan formation and distribution.
30227357	5	13	dep	determine	1183:1191	arg1	c					1180:1180	c	1180:1180	c	1180:1180	These methods, validated to ICH Q2R1 regulatory validation parameters, were successfully applied to- (a) screen cell culture media; (b) determine changes in the metal concentration during cell growth in spinner flasks, and, (c) determine effect on the glycosylation pattern and homogeneity of an IgG3:κ produced from a murine-hybridoma cell line in bench-top parallel bioreactors due to a spike in copper and iron concentration.
30227357	1	14	theme	glycan	387:392	arg1	formation					394:402	glycan formation	387:402	glycan formation	387:402	Metal ions can be enzyme cofactors and can directly influence the kinetics of biochemical reactions that also influence the biological production and quality attributes of therapeutic proteins, such as glycan formation and distribution.
30227357	4	15	from	composition	813:823	arg1	matrices					863:870	different sample matrices	846:870	different sample matrices	846:870	Here, we describe a platform of analytical methods to determine the composition of several metals in different sample matrices using an advanced automated Inductively Coupled Plasma-Mass Spectrometry (ICP-MS).
30227357	4	16	theme	Plasma-Mass	920:930	arg1	ICP-MS					946:951	ICP-MS	946:951	ICP-MS	946:951	Here, we describe a platform of analytical methods to determine the composition of several metals in different sample matrices using an advanced automated Inductively Coupled Plasma-Mass Spectrometry (ICP-MS).
30227357	4	16	theme	Plasma-Mass	920:930	arg1	Spectrometry					932:943	an advanced automated Inductively Coupled Plasma-Mass Spectrometry	878:943	an advanced automated Inductively Coupled Plasma-Mass Spectrometry (ICP-MS)	878:952	Here, we describe a platform of analytical methods to determine the composition of several metals in different sample matrices using an advanced automated Inductively Coupled Plasma-Mass Spectrometry (ICP-MS).
30227357	0	17	from	evaluation	74:83	arg1	concentration					104:116	metal concentration	98:116	metal concentration	98:116	An ICP-MS platform for metal content assessment of cell culture media and evaluation of spikes in metal concentration on the quality of an IgG3:κ monoclonal antibody during production.
30227357	5	18	theme	IgG3	1251:1254	arg1	effect					1193:1198	effect	1193:1198	effect on the glycosylation pattern and homogeneity of an IgG3	1193:1254	These methods, validated to ICH Q2R1 regulatory validation parameters, were successfully applied to- (a) screen cell culture media; (b) determine changes in the metal concentration during cell growth in spinner flasks, and, (c) determine effect on the glycosylation pattern and homogeneity of an IgG3:κ produced from a murine-hybridoma cell line in bench-top parallel bioreactors due to a spike in copper and iron concentration.
30227357	0	19	theme	cell	51:54	arg1	media					64:68	cell culture media	51:68	cell culture media	51:68	An ICP-MS platform for metal content assessment of cell culture media and evaluation of spikes in metal concentration on the quality of an IgG3:κ monoclonal antibody during production.
30227357	0	20	from	platform	10:17	arg1	quality					125:131	the quality	121:131	the quality of an IgG3:κ monoclonal antibody during production	121:182	An ICP-MS platform for metal content assessment of cell culture media and evaluation of spikes in metal concentration on the quality of an IgG3:κ monoclonal antibody during production.
30227357	5	21	theme	glycosylation	1207:1219	arg1	pattern					1221:1227	the glycosylation pattern	1203:1227	the glycosylation pattern	1203:1227	These methods, validated to ICH Q2R1 regulatory validation parameters, were successfully applied to- (a) screen cell culture media; (b) determine changes in the metal concentration during cell growth in spinner flasks, and, (c) determine effect on the glycosylation pattern and homogeneity of an IgG3:κ produced from a murine-hybridoma cell line in bench-top parallel bioreactors due to a spike in copper and iron concentration.
30227357	0	22	theme	media	64:68	arg1	assessment					37:46	metal content assessment	23:46	metal content assessment of cell culture media and evaluation of spikes in metal concentration	23:116	An ICP-MS platform for metal content assessment of cell culture media and evaluation of spikes in metal concentration on the quality of an IgG3:κ monoclonal antibody during production.
30227357	1	23	theme	biological	309:318	arg1	production					320:329	biological production	309:329	biological production	309:329	Metal ions can be enzyme cofactors and can directly influence the kinetics of biochemical reactions that also influence the biological production and quality attributes of therapeutic proteins, such as glycan formation and distribution.
30227357	0	24	theme	evaluation	74:83	arg1	assessment					37:46	metal content assessment	23:46	metal content assessment of cell culture media and evaluation of spikes in metal concentration	23:116	An ICP-MS platform for metal content assessment of cell culture media and evaluation of spikes in metal concentration on the quality of an IgG3:κ monoclonal antibody during production.
30227357	0	25	from	assessment	37:46	arg1	concentration					104:116	metal concentration	98:116	metal concentration	98:116	An ICP-MS platform for metal content assessment of cell culture media and evaluation of spikes in metal concentration on the quality of an IgG3:κ monoclonal antibody during production.
30227357	5	26	theme	murine-hybridoma	1274:1289	arg1	line					1296:1299	a murine-hybridoma cell line	1272:1299	a murine-hybridoma cell line	1272:1299	These methods, validated to ICH Q2R1 regulatory validation parameters, were successfully applied to- (a) screen cell culture media; (b) determine changes in the metal concentration during cell growth in spinner flasks, and, (c) determine effect on the glycosylation pattern and homogeneity of an IgG3:κ produced from a murine-hybridoma cell line in bench-top parallel bioreactors due to a spike in copper and iron concentration.
30227357	0	27	theme	spikes	88:93	arg1	media					64:68	cell culture media	51:68	cell culture media	51:68	An ICP-MS platform for metal content assessment of cell culture media and evaluation of spikes in metal concentration on the quality of an IgG3:κ monoclonal antibody during production.
30227357	0	27	theme	spikes	88:93	arg1	evaluation					74:83	evaluation	74:83	evaluation of spikes in metal concentration	74:116	An ICP-MS platform for metal content assessment of cell culture media and evaluation of spikes in metal concentration on the quality of an IgG3:κ monoclonal antibody during production.
30227357	5	28	theme	Q2R1	987:990	arg1	parameters					1014:1023	ICH Q2R1 regulatory validation parameters	983:1023	ICH Q2R1 regulatory validation parameters	983:1023	These methods, validated to ICH Q2R1 regulatory validation parameters, were successfully applied to- (a) screen cell culture media; (b) determine changes in the metal concentration during cell growth in spinner flasks, and, (c) determine effect on the glycosylation pattern and homogeneity of an IgG3:κ produced from a murine-hybridoma cell line in bench-top parallel bioreactors due to a spike in copper and iron concentration.
30227357	3	29	from	yield	616:620	arg1	composition					678:688	media composition	672:688	media composition	672:688	Because such concentration changes can impact cell growth, manufacturing yield and product quality the alteration/fluctuation in media composition should be well controlled to maintain product quality.
30227357	1	30	dep	production	320:329	arg1	the					305:307	the	305:307	the	305:307	Metal ions can be enzyme cofactors and can directly influence the kinetics of biochemical reactions that also influence the biological production and quality attributes of therapeutic proteins, such as glycan formation and distribution.
30227357	6	31	from	media	1455:1459	arg1	maintenance					1406:1416	maintenance	1406:1416	maintenance of metal content in the cell culture media	1406:1459	Our results show that maintenance of metal content in the cell culture media is critical for product consistency of the IgG3:κ produced.
30227357	5	32	from	spike	1344:1348	arg1	concentration					1369:1381	copper and iron concentration	1353:1381	copper and iron concentration	1353:1381	These methods, validated to ICH Q2R1 regulatory validation parameters, were successfully applied to- (a) screen cell culture media; (b) determine changes in the metal concentration during cell growth in spinner flasks, and, (c) determine effect on the glycosylation pattern and homogeneity of an IgG3:κ produced from a murine-hybridoma cell line in bench-top parallel bioreactors due to a spike in copper and iron concentration.
30227357	2	33	theme	metals	453:458	arg1	concentrations					435:448	the concentrations	431:448	the concentrations of metals in commercially available chemically defined media	431:509	However, the concentrations of metals in commercially available chemically defined media can range from 1 to 25,000 ppb.
30227357	0	34	theme	metal	23:27	arg1	assessment					37:46	metal content assessment	23:46	metal content assessment of cell culture media and evaluation of spikes in metal concentration	23:116	An ICP-MS platform for metal content assessment of cell culture media and evaluation of spikes in metal concentration on the quality of an IgG3:κ monoclonal antibody during production.
30227357	1	35	theme	biochemical	263:273	arg1	reactions					275:283	biochemical reactions	263:283	biochemical reactions that also influence the biological production and quality attributes of therapeutic proteins, such as glycan formation and distribution	263:419	Metal ions can be enzyme cofactors and can directly influence the kinetics of biochemical reactions that also influence the biological production and quality attributes of therapeutic proteins, such as glycan formation and distribution.
30227357	5	36	dep	determine	1091:1099	arg1	b					1088:1088	b	1088:1088	b	1088:1088	These methods, validated to ICH Q2R1 regulatory validation parameters, were successfully applied to- (a) screen cell culture media; (b) determine changes in the metal concentration during cell growth in spinner flasks, and, (c) determine effect on the glycosylation pattern and homogeneity of an IgG3:κ produced from a murine-hybridoma cell line in bench-top parallel bioreactors due to a spike in copper and iron concentration.
30227357	3	37	theme	product	626:632	arg1	quality					634:640	product quality	626:640	product quality	626:640	Because such concentration changes can impact cell growth, manufacturing yield and product quality the alteration/fluctuation in media composition should be well controlled to maintain product quality.
30227357	4	38	theme	several	828:834	arg1	metals					836:841	several metals	828:841	several metals	828:841	Here, we describe a platform of analytical methods to determine the composition of several metals in different sample matrices using an advanced automated Inductively Coupled Plasma-Mass Spectrometry (ICP-MS).
30227357	5	39	theme	cell	1143:1146	arg1	growth					1148:1153	cell growth	1143:1153	cell growth	1143:1153	These methods, validated to ICH Q2R1 regulatory validation parameters, were successfully applied to- (a) screen cell culture media; (b) determine changes in the metal concentration during cell growth in spinner flasks, and, (c) determine effect on the glycosylation pattern and homogeneity of an IgG3:κ produced from a murine-hybridoma cell line in bench-top parallel bioreactors due to a spike in copper and iron concentration.
30227357	5	40	theme	to-	1052:1054	arg1	media					1080:1084	to- (a) screen cell culture media	1052:1084	to- (a) screen cell culture media	1052:1084	These methods, validated to ICH Q2R1 regulatory validation parameters, were successfully applied to- (a) screen cell culture media; (b) determine changes in the metal concentration during cell growth in spinner flasks, and, (c) determine effect on the glycosylation pattern and homogeneity of an IgG3:κ produced from a murine-hybridoma cell line in bench-top parallel bioreactors due to a spike in copper and iron concentration.
30227357	4	41	theme	methods	788:794	arg1	platform					765:772	a platform	763:772	a platform of analytical methods to determine the composition of several metals in different sample matrices using an advanced automated Inductively Coupled Plasma-Mass Spectrometry (ICP-MS)	763:952	Here, we describe a platform of analytical methods to determine the composition of several metals in different sample matrices using an advanced automated Inductively Coupled Plasma-Mass Spectrometry (ICP-MS).
30227357	5	42	theme	validation	1003:1012	arg1	parameters					1014:1023	ICH Q2R1 regulatory validation parameters	983:1023	ICH Q2R1 regulatory validation parameters	983:1023	These methods, validated to ICH Q2R1 regulatory validation parameters, were successfully applied to- (a) screen cell culture media; (b) determine changes in the metal concentration during cell growth in spinner flasks, and, (c) determine effect on the glycosylation pattern and homogeneity of an IgG3:κ produced from a murine-hybridoma cell line in bench-top parallel bioreactors due to a spike in copper and iron concentration.
30227357	5	43	theme	bench-top	1304:1312	arg1	bioreactors					1323:1333	bench-top parallel bioreactors	1304:1333	bench-top parallel bioreactors due to a spike in copper and iron concentration	1304:1381	These methods, validated to ICH Q2R1 regulatory validation parameters, were successfully applied to- (a) screen cell culture media; (b) determine changes in the metal concentration during cell growth in spinner flasks, and, (c) determine effect on the glycosylation pattern and homogeneity of an IgG3:κ produced from a murine-hybridoma cell line in bench-top parallel bioreactors due to a spike in copper and iron concentration.
30227357	6	44	theme	metal	1421:1425	arg1	content					1427:1433	metal content	1421:1433	metal content in the cell culture media	1421:1459	Our results show that maintenance of metal content in the cell culture media is critical for product consistency of the IgG3:κ produced.
30227357	6	45	from	maintenance	1406:1416	arg1	media					1455:1459	the cell culture media	1438:1459	the cell culture media	1438:1459	Our results show that maintenance of metal content in the cell culture media is critical for product consistency of the IgG3:κ produced.
30227357	0	46	theme	κ	144:144	arg1	antibody					157:164	an IgG3:κ monoclonal antibody	136:164	an IgG3:κ monoclonal antibody during production	136:182	An ICP-MS platform for metal content assessment of cell culture media and evaluation of spikes in metal concentration on the quality of an IgG3:κ monoclonal antibody during production.
30227357	0	47	theme	antibody	157:164	arg1	quality					125:131	the quality	121:131	the quality of an IgG3:κ monoclonal antibody during production	121:182	An ICP-MS platform for metal content assessment of cell culture media and evaluation of spikes in metal concentration on the quality of an IgG3:κ monoclonal antibody during production.
30227357	3	48	from	quality	634:640	arg1	composition					678:688	media composition	672:688	media composition	672:688	Because such concentration changes can impact cell growth, manufacturing yield and product quality the alteration/fluctuation in media composition should be well controlled to maintain product quality.
30227357	6	49	theme	product	1477:1483	arg1	consistency					1485:1495	product consistency	1477:1495	product consistency of the IgG3:κ produced	1477:1518	Our results show that maintenance of metal content in the cell culture media is critical for product consistency of the IgG3:κ produced.
30227357	4	50	theme	metals	836:841	arg1	composition					813:823	the composition	809:823	the composition of several metals in different sample matrices	809:870	Here, we describe a platform of analytical methods to determine the composition of several metals in different sample matrices using an advanced automated Inductively Coupled Plasma-Mass Spectrometry (ICP-MS).
30227357	5	51	theme	copper	1353:1358	arg1	concentration					1369:1381	copper and iron concentration	1353:1381	copper and iron concentration	1353:1381	These methods, validated to ICH Q2R1 regulatory validation parameters, were successfully applied to- (a) screen cell culture media; (b) determine changes in the metal concentration during cell growth in spinner flasks, and, (c) determine effect on the glycosylation pattern and homogeneity of an IgG3:κ produced from a murine-hybridoma cell line in bench-top parallel bioreactors due to a spike in copper and iron concentration.
30227357	3	52	theme	such	551:554	arg1	changes					570:576	such concentration changes	551:576	such concentration changes	551:576	Because such concentration changes can impact cell growth, manufacturing yield and product quality the alteration/fluctuation in media composition should be well controlled to maintain product quality.
30227357	4	53	theme	different	846:854	arg1	matrices					863:870	different sample matrices	846:870	different sample matrices	846:870	Here, we describe a platform of analytical methods to determine the composition of several metals in different sample matrices using an advanced automated Inductively Coupled Plasma-Mass Spectrometry (ICP-MS).
30227357	5	54	theme	iron	1364:1367	arg1	concentration					1369:1381	copper and iron concentration	1353:1381	copper and iron concentration	1353:1381	These methods, validated to ICH Q2R1 regulatory validation parameters, were successfully applied to- (a) screen cell culture media; (b) determine changes in the metal concentration during cell growth in spinner flasks, and, (c) determine effect on the glycosylation pattern and homogeneity of an IgG3:κ produced from a murine-hybridoma cell line in bench-top parallel bioreactors due to a spike in copper and iron concentration.
30227357	1	55	theme	proteins	369:376	arg1	production					320:329	biological production	309:329	biological production	309:329	Metal ions can be enzyme cofactors and can directly influence the kinetics of biochemical reactions that also influence the biological production and quality attributes of therapeutic proteins, such as glycan formation and distribution.
30227357	1	55	theme	proteins	369:376	arg1	attributes					343:352	quality attributes	335:352	quality attributes	335:352	Metal ions can be enzyme cofactors and can directly influence the kinetics of biochemical reactions that also influence the biological production and quality attributes of therapeutic proteins, such as glycan formation and distribution.
30227357	6	56	theme	cell	1442:1445	arg1	media					1455:1459	the cell culture media	1438:1459	the cell culture media	1438:1459	Our results show that maintenance of metal content in the cell culture media is critical for product consistency of the IgG3:κ produced.
30227357	5	57	from	effect	1193:1198	arg1	pattern					1221:1227	the glycosylation pattern	1203:1227	the glycosylation pattern	1203:1227	These methods, validated to ICH Q2R1 regulatory validation parameters, were successfully applied to- (a) screen cell culture media; (b) determine changes in the metal concentration during cell growth in spinner flasks, and, (c) determine effect on the glycosylation pattern and homogeneity of an IgG3:κ produced from a murine-hybridoma cell line in bench-top parallel bioreactors due to a spike in copper and iron concentration.
30227357	5	57	from	effect	1193:1198	arg1	homogeneity					1233:1243	homogeneity	1233:1243	homogeneity	1233:1243	These methods, validated to ICH Q2R1 regulatory validation parameters, were successfully applied to- (a) screen cell culture media; (b) determine changes in the metal concentration during cell growth in spinner flasks, and, (c) determine effect on the glycosylation pattern and homogeneity of an IgG3:κ produced from a murine-hybridoma cell line in bench-top parallel bioreactors due to a spike in copper and iron concentration.
30227357	4	58	theme	automated	890:898	arg1	ICP-MS					946:951	ICP-MS	946:951	ICP-MS	946:951	Here, we describe a platform of analytical methods to determine the composition of several metals in different sample matrices using an advanced automated Inductively Coupled Plasma-Mass Spectrometry (ICP-MS).
30227357	4	58	theme	automated	890:898	arg1	Spectrometry					932:943	an advanced automated Inductively Coupled Plasma-Mass Spectrometry	878:943	an advanced automated Inductively Coupled Plasma-Mass Spectrometry (ICP-MS)	878:952	Here, we describe a platform of analytical methods to determine the composition of several metals in different sample matrices using an advanced automated Inductively Coupled Plasma-Mass Spectrometry (ICP-MS).
30227357	2	59	from	media	505:509	arg1	concentrations					435:448	the concentrations	431:448	the concentrations of metals in commercially available chemically defined media	431:509	However, the concentrations of metals in commercially available chemically defined media can range from 1 to 25,000 ppb.
30227357	0	60	theme	IgG3	139:142	arg1	antibody					157:164	an IgG3:κ monoclonal antibody	136:164	an IgG3:κ monoclonal antibody during production	136:182	An ICP-MS platform for metal content assessment of cell culture media and evaluation of spikes in metal concentration on the quality of an IgG3:κ monoclonal antibody during production.
30227357	5	61	theme	a	1057:1057	arg1	media					1080:1084	to- (a) screen cell culture media	1052:1084	to- (a) screen cell culture media	1052:1084	These methods, validated to ICH Q2R1 regulatory validation parameters, were successfully applied to- (a) screen cell culture media; (b) determine changes in the metal concentration during cell growth in spinner flasks, and, (c) determine effect on the glycosylation pattern and homogeneity of an IgG3:κ produced from a murine-hybridoma cell line in bench-top parallel bioreactors due to a spike in copper and iron concentration.
30227357	3	62	theme	manufacturing	602:614	arg1	yield					616:620	manufacturing yield	602:620	manufacturing yield	602:620	Because such concentration changes can impact cell growth, manufacturing yield and product quality the alteration/fluctuation in media composition should be well controlled to maintain product quality.
30227357	6	63	theme	κ	1509:1509	arg1	consistency					1485:1495	product consistency	1477:1495	product consistency of the IgG3:κ produced	1477:1518	Our results show that maintenance of metal content in the cell culture media is critical for product consistency of the IgG3:κ produced.
30227357	4	64	theme	Coupled	912:918	arg1	ICP-MS					946:951	ICP-MS	946:951	ICP-MS	946:951	Here, we describe a platform of analytical methods to determine the composition of several metals in different sample matrices using an advanced automated Inductively Coupled Plasma-Mass Spectrometry (ICP-MS).
30227357	4	64	theme	Coupled	912:918	arg1	Spectrometry					932:943	an advanced automated Inductively Coupled Plasma-Mass Spectrometry	878:943	an advanced automated Inductively Coupled Plasma-Mass Spectrometry (ICP-MS)	878:952	Here, we describe a platform of analytical methods to determine the composition of several metals in different sample matrices using an advanced automated Inductively Coupled Plasma-Mass Spectrometry (ICP-MS).
30227357	0	65	theme	culture	56:62	arg1	media					64:68	cell culture media	51:68	cell culture media	51:68	An ICP-MS platform for metal content assessment of cell culture media and evaluation of spikes in metal concentration on the quality of an IgG3:κ monoclonal antibody during production.
30227357	5	66	theme	screen	1060:1065	arg1	media					1080:1084	to- (a) screen cell culture media	1052:1084	to- (a) screen cell culture media	1052:1084	These methods, validated to ICH Q2R1 regulatory validation parameters, were successfully applied to- (a) screen cell culture media; (b) determine changes in the metal concentration during cell growth in spinner flasks, and, (c) determine effect on the glycosylation pattern and homogeneity of an IgG3:κ produced from a murine-hybridoma cell line in bench-top parallel bioreactors due to a spike in copper and iron concentration.
30227357	6	67	theme	IgG3	1504:1507	arg1	κ					1509:1509	the IgG3:κ	1500:1509	the IgG3:κ produced	1500:1518	Our results show that maintenance of metal content in the cell culture media is critical for product consistency of the IgG3:κ produced.
30227357	2	68	theme	available	476:484	arg1	media					505:509	commercially available chemically defined media	463:509	commercially available chemically defined media	463:509	However, the concentrations of metals in commercially available chemically defined media can range from 1 to 25,000 ppb.
30227357	5	69	theme	cell	1067:1070	arg1	media					1080:1084	to- (a) screen cell culture media	1052:1084	to- (a) screen cell culture media	1052:1084	These methods, validated to ICH Q2R1 regulatory validation parameters, were successfully applied to- (a) screen cell culture media; (b) determine changes in the metal concentration during cell growth in spinner flasks, and, (c) determine effect on the glycosylation pattern and homogeneity of an IgG3:κ produced from a murine-hybridoma cell line in bench-top parallel bioreactors due to a spike in copper and iron concentration.
30227357	5	70	theme	metal	1116:1120	arg1	concentration					1122:1134	the metal concentration	1112:1134	the metal concentration during cell growth	1112:1153	These methods, validated to ICH Q2R1 regulatory validation parameters, were successfully applied to- (a) screen cell culture media; (b) determine changes in the metal concentration during cell growth in spinner flasks, and, (c) determine effect on the glycosylation pattern and homogeneity of an IgG3:κ produced from a murine-hybridoma cell line in bench-top parallel bioreactors due to a spike in copper and iron concentration.
30227357	2	71	from	concentrations	435:448	arg1	media					505:509	commercially available chemically defined media	463:509	commercially available chemically defined media	463:509	However, the concentrations of metals in commercially available chemically defined media can range from 1 to 25,000 ppb.
30227357	1	72	theme	quality	335:341	arg1	attributes					343:352	quality attributes	335:352	quality attributes	335:352	Metal ions can be enzyme cofactors and can directly influence the kinetics of biochemical reactions that also influence the biological production and quality attributes of therapeutic proteins, such as glycan formation and distribution.
30227357	5	73	theme	ICH	983:985	arg1	parameters					1014:1023	ICH Q2R1 regulatory validation parameters	983:1023	ICH Q2R1 regulatory validation parameters	983:1023	These methods, validated to ICH Q2R1 regulatory validation parameters, were successfully applied to- (a) screen cell culture media; (b) determine changes in the metal concentration during cell growth in spinner flasks, and, (c) determine effect on the glycosylation pattern and homogeneity of an IgG3:κ produced from a murine-hybridoma cell line in bench-top parallel bioreactors due to a spike in copper and iron concentration.
30227357	2	74	theme	defined	497:503	arg1	media					505:509	commercially available chemically defined media	463:509	commercially available chemically defined media	463:509	However, the concentrations of metals in commercially available chemically defined media can range from 1 to 25,000 ppb.
30227357	2	75	from	metals	453:458	arg1	media					505:509	commercially available chemically defined media	463:509	commercially available chemically defined media	463:509	However, the concentrations of metals in commercially available chemically defined media can range from 1 to 25,000 ppb.
30227357	5	76	theme	cell	1291:1294	arg1	line					1296:1299	a murine-hybridoma cell line	1272:1299	a murine-hybridoma cell line	1272:1299	These methods, validated to ICH Q2R1 regulatory validation parameters, were successfully applied to- (a) screen cell culture media; (b) determine changes in the metal concentration during cell growth in spinner flasks, and, (c) determine effect on the glycosylation pattern and homogeneity of an IgG3:κ produced from a murine-hybridoma cell line in bench-top parallel bioreactors due to a spike in copper and iron concentration.
30227357	0	77	theme	ICP-MS	3:8	arg1	platform					10:17	An ICP-MS platform	0:17	An ICP-MS platform for metal content assessment of cell culture media and evaluation of spikes in metal concentration on the quality of an IgG3:κ monoclonal antibody during production.	0:183	An ICP-MS platform for metal content assessment of cell culture media and evaluation of spikes in metal concentration on the quality of an IgG3:κ monoclonal antibody during production.
30227357	5	78	theme	culture	1072:1078	arg1	media					1080:1084	to- (a) screen cell culture media	1052:1084	to- (a) screen cell culture media	1052:1084	These methods, validated to ICH Q2R1 regulatory validation parameters, were successfully applied to- (a) screen cell culture media; (b) determine changes in the metal concentration during cell growth in spinner flasks, and, (c) determine effect on the glycosylation pattern and homogeneity of an IgG3:κ produced from a murine-hybridoma cell line in bench-top parallel bioreactors due to a spike in copper and iron concentration.
30227357	5	79	theme	spinner	1158:1164	arg1	flasks					1166:1171	spinner flasks	1158:1171	spinner flasks	1158:1171	These methods, validated to ICH Q2R1 regulatory validation parameters, were successfully applied to- (a) screen cell culture media; (b) determine changes in the metal concentration during cell growth in spinner flasks, and, (c) determine effect on the glycosylation pattern and homogeneity of an IgG3:κ produced from a murine-hybridoma cell line in bench-top parallel bioreactors due to a spike in copper and iron concentration.
30227357	0	80	theme	content	29:35	arg1	assessment					37:46	metal content assessment	23:46	metal content assessment of cell culture media and evaluation of spikes in metal concentration	23:116	An ICP-MS platform for metal content assessment of cell culture media and evaluation of spikes in metal concentration on the quality of an IgG3:κ monoclonal antibody during production.
30227357	6	81	from	content	1427:1433	arg1	media					1455:1459	the cell culture media	1438:1459	the cell culture media	1438:1459	Our results show that maintenance of metal content in the cell culture media is critical for product consistency of the IgG3:κ produced.
30227357	0	82	from	concentration	104:116	arg1	assessment					37:46	metal content assessment	23:46	metal content assessment of cell culture media and evaluation of spikes in metal concentration	23:116	An ICP-MS platform for metal content assessment of cell culture media and evaluation of spikes in metal concentration on the quality of an IgG3:κ monoclonal antibody during production.
30227357	5	83	theme	due	1335:1337	arg1	bioreactors					1323:1333	bench-top parallel bioreactors	1304:1333	bench-top parallel bioreactors due to a spike in copper and iron concentration	1304:1381	These methods, validated to ICH Q2R1 regulatory validation parameters, were successfully applied to- (a) screen cell culture media; (b) determine changes in the metal concentration during cell growth in spinner flasks, and, (c) determine effect on the glycosylation pattern and homogeneity of an IgG3:κ produced from a murine-hybridoma cell line in bench-top parallel bioreactors due to a spike in copper and iron concentration.
30227357	5	84	from	changes	1101:1107	arg1	concentration					1122:1134	the metal concentration	1112:1134	the metal concentration during cell growth	1112:1153	These methods, validated to ICH Q2R1 regulatory validation parameters, were successfully applied to- (a) screen cell culture media; (b) determine changes in the metal concentration during cell growth in spinner flasks, and, (c) determine effect on the glycosylation pattern and homogeneity of an IgG3:κ produced from a murine-hybridoma cell line in bench-top parallel bioreactors due to a spike in copper and iron concentration.
30227357	5	84	from	changes	1101:1107	arg1	flasks					1166:1171	spinner flasks	1158:1171	spinner flasks	1158:1171	These methods, validated to ICH Q2R1 regulatory validation parameters, were successfully applied to- (a) screen cell culture media; (b) determine changes in the metal concentration during cell growth in spinner flasks, and, (c) determine effect on the glycosylation pattern and homogeneity of an IgG3:κ produced from a murine-hybridoma cell line in bench-top parallel bioreactors due to a spike in copper and iron concentration.
30227357	1	85	theme	reactions	275:283	arg1	kinetics					251:258	the kinetics	247:258	the kinetics of biochemical reactions that also influence the biological production and quality attributes of therapeutic proteins, such as glycan formation and distribution	247:419	Metal ions can be enzyme cofactors and can directly influence the kinetics of biochemical reactions that also influence the biological production and quality attributes of therapeutic proteins, such as glycan formation and distribution.
30227357	3	86	theme	media	672:676	arg1	composition					678:688	media composition	672:688	media composition	672:688	Because such concentration changes can impact cell growth, manufacturing yield and product quality the alteration/fluctuation in media composition should be well controlled to maintain product quality.
30227357	5	87	theme	regulatory	992:1001	arg1	parameters					1014:1023	ICH Q2R1 regulatory validation parameters	983:1023	ICH Q2R1 regulatory validation parameters	983:1023	These methods, validated to ICH Q2R1 regulatory validation parameters, were successfully applied to- (a) screen cell culture media; (b) determine changes in the metal concentration during cell growth in spinner flasks, and, (c) determine effect on the glycosylation pattern and homogeneity of an IgG3:κ produced from a murine-hybridoma cell line in bench-top parallel bioreactors due to a spike in copper and iron concentration.
30227357	4	88	theme	analytical	777:786	arg1	methods					788:794	analytical methods	777:794	analytical methods	777:794	Here, we describe a platform of analytical methods to determine the composition of several metals in different sample matrices using an advanced automated Inductively Coupled Plasma-Mass Spectrometry (ICP-MS).
30227357	6	89	theme	content	1427:1433	arg1	maintenance					1406:1416	maintenance	1406:1416	maintenance of metal content in the cell culture media	1406:1459	Our results show that maintenance of metal content in the cell culture media is critical for product consistency of the IgG3:κ produced.
30227357	3	90	dep	yield	616:620	arg1	alteration/fluctuation					646:667	the alteration/fluctuation	642:667	the alteration/fluctuation	642:667	Because such concentration changes can impact cell growth, manufacturing yield and product quality the alteration/fluctuation in media composition should be well controlled to maintain product quality.
30227357	2	91	from	25,000 ppb	531:540	arg1	range					515:519	range	515:519	range	515:519	However, the concentrations of metals in commercially available chemically defined media can range from 1 to 25,000 ppb.
30227357	5	92	theme	parallel	1314:1321	arg1	bioreactors					1323:1333	bench-top parallel bioreactors	1304:1333	bench-top parallel bioreactors due to a spike in copper and iron concentration	1304:1381	These methods, validated to ICH Q2R1 regulatory validation parameters, were successfully applied to- (a) screen cell culture media; (b) determine changes in the metal concentration during cell growth in spinner flasks, and, (c) determine effect on the glycosylation pattern and homogeneity of an IgG3:κ produced from a murine-hybridoma cell line in bench-top parallel bioreactors due to a spike in copper and iron concentration.
30227357	1	93	theme	enzyme	203:208	arg1	cofactors					210:218	enzyme cofactors	203:218	enzyme cofactors	203:218	Metal ions can be enzyme cofactors and can directly influence the kinetics of biochemical reactions that also influence the biological production and quality attributes of therapeutic proteins, such as glycan formation and distribution.
31205417	8	0	theme	significant	1068:1078	arg1	changes					1080:1086	significant changes	1068:1086	significant changes in the carbohydrases complex of the strain	1068:1129	It was established that gamma mutagenesis resulted in significant changes in the carbohydrases complex of the strain.
31205417	8	1	theme	gamma	1038:1042	arg1	mutagenesis					1044:1054	gamma mutagenesis	1038:1054	gamma mutagenesis	1038:1054	It was established that gamma mutagenesis resulted in significant changes in the carbohydrases complex of the strain.
31205417	11	2	theme	reesei	1530:1535	arg1	complex					1550:1556	T. reesei carbohydrase complex	1527:1556	T. reesei carbohydrase complex	1527:1556	The obtained data show the ability of gamma irradiation to affect the component composition of T. reesei carbohydrase complex.
31205417	0	3	from	Change	4:9	arg1	Composition					18:28	the Composition	14:28	the Composition of Trichoderma reesei	14:50	The Change in the Composition of Trichoderma reesei Carbohydrases Complex as a Result of Gamma Mutagenesis.
31205417	5	4	theme	strain	675:680	arg1	activity					659:666	Endoglucanase activity	645:666	Endoglucanase activity of the strain	645:680	Endoglucanase activity of the strain was increased 5-fold, while xylanase activity increased more than 8-fold.
31205417	7	5	theme	freeze	1000:1005	arg1	dryer					1007:1011	freeze dryer	1000:1011	freeze dryer	1000:1011	So, the strains were cultured in laboratory fermenters; concentrated preparations were obtained using freeze dryer.
31205417	9	6	theme	mutant	1244:1249	arg1	complex					1229:1235	the enzyme complex	1218:1235	the enzyme complex of the mutant	1218:1249	Cellobiohydrolase I being the major carbohydrase in the original strain was absent in the enzyme complex of the mutant.
31205417	9	7	theme	enzyme	1222:1227	arg1	complex					1229:1235	the enzyme complex	1218:1235	the enzyme complex of the mutant	1218:1249	Cellobiohydrolase I being the major carbohydrase in the original strain was absent in the enzyme complex of the mutant.
31205417	1	8	dep	filamentous	112:122	arg1	fungus					124:129	fungus	124:129	fungus	124:129	The filamentous fungus Trichoderma reesei is traditionally used as the main industrial producer of cellulases and hemicellulases.
31205417	10	9	theme	endoglucanases	1279:1292	arg1	share					1256:1260	The share	1252:1260	The share of xylanases and endoglucanases in the preparation from the mutant strain	1252:1334	The share of xylanases and endoglucanases in the preparation from the mutant strain increased by 6% and 6.5%, respectively, compared with the preparation from the original strain.
31205417	4	10	theme	endodepolymerases	604:620	arg1	proportion					590:599	the proportion	586:599	the proportion of endodepolymerases in its enzyme complex	586:642	Earlier, we carried out gamma mutagenesis of an industrial strain T. reesei BCM18.2/KK to increase the proportion of endodepolymerases in its enzyme complex.
31205417	10	11	from	share	1256:1260	arg1	strain					1329:1334	the mutant strain	1318:1334	the mutant strain	1318:1334	The share of xylanases and endoglucanases in the preparation from the mutant strain increased by 6% and 6.5%, respectively, compared with the preparation from the original strain.
31205417	10	11	from	share	1256:1260	arg1	preparation					1301:1311	the preparation	1297:1311	the preparation from the mutant strain	1297:1334	The share of xylanases and endoglucanases in the preparation from the mutant strain increased by 6% and 6.5%, respectively, compared with the preparation from the original strain.
31205417	11	12	theme	carbohydrase	1537:1548	arg1	complex					1550:1556	T. reesei carbohydrase complex	1527:1556	T. reesei carbohydrase complex	1527:1556	The obtained data show the ability of gamma irradiation to affect the component composition of T. reesei carbohydrase complex.
31205417	2	13	theme	nonstarch	291:299	arg1	polysaccharides					301:315	nonstarch polysaccharides	291:315	nonstarch polysaccharides of cereals	291:326	Recently, the relevance of carbohydrases hydrolyzing nonstarch polysaccharides of cereals has significantly increased in feed production.
31205417	0	14	theme	Mutagenesis	95:105	arg1	Complex					66:72	Complex	66:72	Complex	66:72	The Change in the Composition of Trichoderma reesei Carbohydrases Complex as a Result of Gamma Mutagenesis.
31205417	0	14	theme	Mutagenesis	95:105	arg1	Result					79:84	a Result	77:84	a Result of Gamma Mutagenesis	77:105	The Change in the Composition of Trichoderma reesei Carbohydrases Complex as a Result of Gamma Mutagenesis.
31205417	8	15	theme	carbohydrases	1095:1107	arg1	complex					1109:1115	the carbohydrases complex	1091:1115	the carbohydrases complex of the strain	1091:1129	It was established that gamma mutagenesis resulted in significant changes in the carbohydrases complex of the strain.
31205417	11	16	theme	complex	1550:1556	arg1	composition					1512:1522	the component composition	1498:1522	the component composition of T. reesei carbohydrase complex	1498:1556	The obtained data show the ability of gamma irradiation to affect the component composition of T. reesei carbohydrase complex.
31205417	10	17	theme	mutant	1322:1327	arg1	strain					1329:1334	the mutant strain	1318:1334	the mutant strain	1318:1334	The share of xylanases and endoglucanases in the preparation from the mutant strain increased by 6% and 6.5%, respectively, compared with the preparation from the original strain.
31205417	9	18	from	absent	1208:1213	arg1	complex					1229:1235	the enzyme complex	1218:1235	the enzyme complex of the mutant	1218:1249	Cellobiohydrolase I being the major carbohydrase in the original strain was absent in the enzyme complex of the mutant.
31205417	0	19	theme	Gamma	89:93	arg1	Mutagenesis					95:105	Gamma Mutagenesis	89:105	Gamma Mutagenesis	89:105	The Change in the Composition of Trichoderma reesei Carbohydrases Complex as a Result of Gamma Mutagenesis.
31205417	5	20	theme	Endoglucanase	645:657	arg1	activity					659:666	Endoglucanase activity	645:666	Endoglucanase activity of the strain	645:680	Endoglucanase activity of the strain was increased 5-fold, while xylanase activity increased more than 8-fold.
31205417	6	21	theme	enzyme	821:826	arg1	preparations					828:839	enzyme preparations	821:839	enzyme preparations obtained from the original and mutant T. reesei strains	821:895	It was interesting to determine the carbohydrases composition in enzyme preparations obtained from the original and mutant T. reesei strains.
31205417	2	22	theme	carbohydrases	265:277	arg1	relevance					252:260	the relevance	248:260	the relevance of carbohydrases hydrolyzing nonstarch polysaccharides of cereals	248:326	Recently, the relevance of carbohydrases hydrolyzing nonstarch polysaccharides of cereals has significantly increased in feed production.
31205417	11	23	theme	obtained	1436:1443	arg1	data					1445:1448	The obtained data	1432:1448	The obtained data	1432:1448	The obtained data show the ability of gamma irradiation to affect the component composition of T. reesei carbohydrase complex.
31205417	4	24	from	proportion	590:599	arg1	complex					636:642	its enzyme complex	625:642	its enzyme complex	625:642	Earlier, we carried out gamma mutagenesis of an industrial strain T. reesei BCM18.2/KK to increase the proportion of endodepolymerases in its enzyme complex.
31205417	3	25	theme	materials	403:411	arg1	processing					379:388	processing	379:388	processing of grain raw materials	379:411	In processing of grain raw materials, endodepolymerases, mainly xylanases and endoglucanases, play a key role.
31205417	7	26	theme	concentrated	954:965	arg1	preparations					967:978	concentrated preparations	954:978	concentrated preparations	954:978	So, the strains were cultured in laboratory fermenters; concentrated preparations were obtained using freeze dryer.
31205417	10	27	from	strain	1424:1429	arg1	preparation					1394:1404	the preparation	1390:1404	the preparation from the original strain	1390:1429	The share of xylanases and endoglucanases in the preparation from the mutant strain increased by 6% and 6.5%, respectively, compared with the preparation from the original strain.
31205417	6	28	theme	original	859:866	arg1	strains					889:895	the original and mutant T. reesei strains	855:895	the original and mutant T. reesei strains	855:895	It was interesting to determine the carbohydrases composition in enzyme preparations obtained from the original and mutant T. reesei strains.
31205417	11	29	theme	gamma	1470:1474	arg1	irradiation					1476:1486	gamma irradiation	1470:1486	gamma irradiation	1470:1486	The obtained data show the ability of gamma irradiation to affect the component composition of T. reesei carbohydrase complex.
31205417	1	30	theme	main	179:182	arg1	producer					195:202	the main industrial producer	175:202	the main industrial producer of cellulases and hemicellulases	175:235	The filamentous fungus Trichoderma reesei is traditionally used as the main industrial producer of cellulases and hemicellulases.
31205417	1	30	theme	main	179:182	arg1	reesei					143:148	The filamentous fungus Trichoderma reesei	108:148	The filamentous fungus Trichoderma reesei	108:148	The filamentous fungus Trichoderma reesei is traditionally used as the main industrial producer of cellulases and hemicellulases.
31205417	3	31	theme	grain	393:397	arg1	materials					403:411	grain raw materials	393:411	grain raw materials	393:411	In processing of grain raw materials, endodepolymerases, mainly xylanases and endoglucanases, play a key role.
31205417	8	32	from	changes	1080:1086	arg1	complex					1109:1115	the carbohydrases complex	1091:1115	the carbohydrases complex of the strain	1091:1129	It was established that gamma mutagenesis resulted in significant changes in the carbohydrases complex of the strain.
31205417	8	33	theme	strain	1124:1129	arg1	complex					1109:1115	the carbohydrases complex	1091:1115	the carbohydrases complex of the strain	1091:1129	It was established that gamma mutagenesis resulted in significant changes in the carbohydrases complex of the strain.
31205417	6	34	theme	carbohydrases	792:804	arg1	composition					806:816	the carbohydrases composition	788:816	the carbohydrases composition in enzyme preparations obtained from the original and mutant T. reesei strains	788:895	It was interesting to determine the carbohydrases composition in enzyme preparations obtained from the original and mutant T. reesei strains.
31205417	2	35	theme	cereals	320:326	arg1	polysaccharides					301:315	nonstarch polysaccharides	291:315	nonstarch polysaccharides of cereals	291:326	Recently, the relevance of carbohydrases hydrolyzing nonstarch polysaccharides of cereals has significantly increased in feed production.
31205417	11	36	theme	irradiation	1476:1486	arg1	ability					1459:1465	the ability	1455:1465	the ability of gamma irradiation to affect the component composition of T. reesei carbohydrase complex	1455:1556	The obtained data show the ability of gamma irradiation to affect the component composition of T. reesei carbohydrase complex.
31205417	1	37	theme	industrial	184:193	arg1	producer					195:202	the main industrial producer	175:202	the main industrial producer of cellulases and hemicellulases	175:235	The filamentous fungus Trichoderma reesei is traditionally used as the main industrial producer of cellulases and hemicellulases.
31205417	1	37	theme	industrial	184:193	arg1	reesei					143:148	The filamentous fungus Trichoderma reesei	108:148	The filamentous fungus Trichoderma reesei	108:148	The filamentous fungus Trichoderma reesei is traditionally used as the main industrial producer of cellulases and hemicellulases.
31205417	3	38	theme	raw	399:401	arg1	materials					403:411	grain raw materials	393:411	grain raw materials	393:411	In processing of grain raw materials, endodepolymerases, mainly xylanases and endoglucanases, play a key role.
31205417	9	39	from	complex	1229:1235	arg1	absent					1208:1213	absent	1208:1213	absent	1208:1213	Cellobiohydrolase I being the major carbohydrase in the original strain was absent in the enzyme complex of the mutant.
31205417	9	40	theme	major	1162:1166	arg1	carbohydrase					1168:1179	the major carbohydrase	1158:1179	the major carbohydrase in the original strain	1158:1202	Cellobiohydrolase I being the major carbohydrase in the original strain was absent in the enzyme complex of the mutant.
31205417	9	41	from	carbohydrase	1168:1179	arg1	strain					1197:1202	the original strain	1184:1202	the original strain	1184:1202	Cellobiohydrolase I being the major carbohydrase in the original strain was absent in the enzyme complex of the mutant.
31205417	4	42	theme	industrial	535:544	arg1	strain					546:551	an industrial strain	532:551	an industrial strain	532:551	Earlier, we carried out gamma mutagenesis of an industrial strain T. reesei BCM18.2/KK to increase the proportion of endodepolymerases in its enzyme complex.
31205417	3	43	theme	key	477:479	arg1	role					481:484	a key role	475:484	a key role	475:484	In processing of grain raw materials, endodepolymerases, mainly xylanases and endoglucanases, play a key role.
31205417	0	44	theme	reesei	45:50	arg1	Composition					18:28	the Composition	14:28	the Composition of Trichoderma reesei	14:50	The Change in the Composition of Trichoderma reesei Carbohydrases Complex as a Result of Gamma Mutagenesis.
31205417	6	45	theme	reesei	882:887	arg1	strains					889:895	the original and mutant T. reesei strains	855:895	the original and mutant T. reesei strains	855:895	It was interesting to determine the carbohydrases composition in enzyme preparations obtained from the original and mutant T. reesei strains.
31205417	10	46	from	strain	1329:1334	arg1	share					1256:1260	The share	1252:1260	The share of xylanases and endoglucanases in the preparation from the mutant strain	1252:1334	The share of xylanases and endoglucanases in the preparation from the mutant strain increased by 6% and 6.5%, respectively, compared with the preparation from the original strain.
31205417	10	46	from	strain	1329:1334	arg1	preparation					1301:1311	the preparation	1297:1311	the preparation from the mutant strain	1297:1334	The share of xylanases and endoglucanases in the preparation from the mutant strain increased by 6% and 6.5%, respectively, compared with the preparation from the original strain.
31205417	10	47	dep	increased	1336:1344	arg1	compared					1376:1383	compared	1376:1383	compared with the preparation from the original strain	1376:1429	The share of xylanases and endoglucanases in the preparation from the mutant strain increased by 6% and 6.5%, respectively, compared with the preparation from the original strain.
31205417	6	48	theme	T.	879:880	arg1	strains					889:895	the original and mutant T. reesei strains	855:895	the original and mutant T. reesei strains	855:895	It was interesting to determine the carbohydrases composition in enzyme preparations obtained from the original and mutant T. reesei strains.
31205417	2	49	theme	feed	359:362	arg1	production					364:373	feed production	359:373	feed production	359:373	Recently, the relevance of carbohydrases hydrolyzing nonstarch polysaccharides of cereals has significantly increased in feed production.
31205417	7	50	theme	laboratory	931:940	arg1	fermenters					942:951	laboratory fermenters	931:951	laboratory fermenters	931:951	So, the strains were cultured in laboratory fermenters; concentrated preparations were obtained using freeze dryer.
31205417	4	51	theme	strain	546:551	arg1	mutagenesis					517:527	gamma mutagenesis	511:527	gamma mutagenesis of an industrial strain T. reesei	511:561	Earlier, we carried out gamma mutagenesis of an industrial strain T. reesei BCM18.2/KK to increase the proportion of endodepolymerases in its enzyme complex.
31205417	6	52	theme	mutant	872:877	arg1	strains					889:895	the original and mutant T. reesei strains	855:895	the original and mutant T. reesei strains	855:895	It was interesting to determine the carbohydrases composition in enzyme preparations obtained from the original and mutant T. reesei strains.
31205417	9	53	theme	original	1188:1195	arg1	strain					1197:1202	the original strain	1184:1202	the original strain	1184:1202	Cellobiohydrolase I being the major carbohydrase in the original strain was absent in the enzyme complex of the mutant.
31205417	1	54	theme	filamentous	112:122	arg1	reesei					143:148	The filamentous fungus Trichoderma reesei	108:148	The filamentous fungus Trichoderma reesei	108:148	The filamentous fungus Trichoderma reesei is traditionally used as the main industrial producer of cellulases and hemicellulases.
31205417	1	54	theme	filamentous	112:122	arg1	producer					195:202	the main industrial producer	175:202	the main industrial producer of cellulases and hemicellulases	175:235	The filamentous fungus Trichoderma reesei is traditionally used as the main industrial producer of cellulases and hemicellulases.
31205417	10	55	theme	xylanases	1265:1273	arg1	share					1256:1260	The share	1252:1260	The share of xylanases and endoglucanases in the preparation from the mutant strain	1252:1334	The share of xylanases and endoglucanases in the preparation from the mutant strain increased by 6% and 6.5%, respectively, compared with the preparation from the original strain.
31205417	5	56	theme	xylanase	710:717	arg1	activity					719:726	xylanase activity	710:726	xylanase activity	710:726	Endoglucanase activity of the strain was increased 5-fold, while xylanase activity increased more than 8-fold.
31205417	1	57	theme	hemicellulases	222:235	arg1	producer					195:202	the main industrial producer	175:202	the main industrial producer of cellulases and hemicellulases	175:235	The filamentous fungus Trichoderma reesei is traditionally used as the main industrial producer of cellulases and hemicellulases.
31205417	1	57	theme	hemicellulases	222:235	arg1	reesei					143:148	The filamentous fungus Trichoderma reesei	108:148	The filamentous fungus Trichoderma reesei	108:148	The filamentous fungus Trichoderma reesei is traditionally used as the main industrial producer of cellulases and hemicellulases.
31205417	1	58	theme	cellulases	207:216	arg1	producer					195:202	the main industrial producer	175:202	the main industrial producer of cellulases and hemicellulases	175:235	The filamentous fungus Trichoderma reesei is traditionally used as the main industrial producer of cellulases and hemicellulases.
31205417	1	58	theme	cellulases	207:216	arg1	reesei					143:148	The filamentous fungus Trichoderma reesei	108:148	The filamentous fungus Trichoderma reesei	108:148	The filamentous fungus Trichoderma reesei is traditionally used as the main industrial producer of cellulases and hemicellulases.
31205417	6	59	from	composition	806:816	arg1	preparations					828:839	enzyme preparations	821:839	enzyme preparations obtained from the original and mutant T. reesei strains	821:895	It was interesting to determine the carbohydrases composition in enzyme preparations obtained from the original and mutant T. reesei strains.
31205417	1	60	theme	Trichoderma	131:141	arg1	reesei					143:148	The filamentous fungus Trichoderma reesei	108:148	The filamentous fungus Trichoderma reesei	108:148	The filamentous fungus Trichoderma reesei is traditionally used as the main industrial producer of cellulases and hemicellulases.
31205417	1	60	theme	Trichoderma	131:141	arg1	producer					195:202	the main industrial producer	175:202	the main industrial producer of cellulases and hemicellulases	175:235	The filamentous fungus Trichoderma reesei is traditionally used as the main industrial producer of cellulases and hemicellulases.
31205417	11	61	theme	component	1502:1510	arg1	composition					1512:1522	the component composition	1498:1522	the component composition of T. reesei carbohydrase complex	1498:1556	The obtained data show the ability of gamma irradiation to affect the component composition of T. reesei carbohydrase complex.
31205417	10	62	theme	original	1415:1422	arg1	strain					1424:1429	the original strain	1411:1429	the original strain	1411:1429	The share of xylanases and endoglucanases in the preparation from the mutant strain increased by 6% and 6.5%, respectively, compared with the preparation from the original strain.
31205417	4	63	theme	gamma	511:515	arg1	mutagenesis					517:527	gamma mutagenesis	511:527	gamma mutagenesis of an industrial strain T. reesei	511:561	Earlier, we carried out gamma mutagenesis of an industrial strain T. reesei BCM18.2/KK to increase the proportion of endodepolymerases in its enzyme complex.
31205417	1	64	used	used	167:170	arg2	reesei					143:148	The filamentous fungus Trichoderma reesei	108:148	The filamentous fungus Trichoderma reesei	108:148	The filamentous fungus Trichoderma reesei is traditionally used as the main industrial producer of cellulases and hemicellulases.
31205417	1	64	used	used	167:170	arg2	producer					195:202	the main industrial producer	175:202	the main industrial producer of cellulases and hemicellulases	175:235	The filamentous fungus Trichoderma reesei is traditionally used as the main industrial producer of cellulases and hemicellulases.
31205417	11	65	theme	T.	1527:1528	arg1	complex					1550:1556	T. reesei carbohydrase complex	1527:1556	T. reesei carbohydrase complex	1527:1556	The obtained data show the ability of gamma irradiation to affect the component composition of T. reesei carbohydrase complex.
31205417	4	66	theme	enzyme	629:634	arg1	complex					636:642	its enzyme complex	625:642	its enzyme complex	625:642	Earlier, we carried out gamma mutagenesis of an industrial strain T. reesei BCM18.2/KK to increase the proportion of endodepolymerases in its enzyme complex.
30828323	4	0	from	beet	684:687	arg1	derivative					703:712	a pectic derivative	694:712	a pectic derivative	694:712	For this purpose we performed in vitro fermentations of nine structurally diverse pectins from citrus fruits and sugar beet, and a pectic derivative, rhamnogalacturonan I (RGI), using a TIM-2 colon model.
30828323	4	0	from	beet	684:687	arg1	pectins					647:653	nine structurally diverse pectins	621:653	nine structurally diverse pectins from citrus fruits and sugar beet	621:687	For this purpose we performed in vitro fermentations of nine structurally diverse pectins from citrus fruits and sugar beet, and a pectic derivative, rhamnogalacturonan I (RGI), using a TIM-2 colon model.
30828323	4	0	from	beet	684:687	arg1	fermentations					604:616	in vitro fermentations	595:616	in vitro fermentations of nine structurally diverse pectins from citrus fruits and sugar beet	595:687	For this purpose we performed in vitro fermentations of nine structurally diverse pectins from citrus fruits and sugar beet, and a pectic derivative, rhamnogalacturonan I (RGI), using a TIM-2 colon model.
30828323	4	0	from	beet	684:687	arg1	I					734:734	rhamnogalacturonan I	715:734	rhamnogalacturonan I (RGI)	715:740	For this purpose we performed in vitro fermentations of nine structurally diverse pectins from citrus fruits and sugar beet, and a pectic derivative, rhamnogalacturonan I (RGI), using a TIM-2 colon model.
30828323	12	1	theme	dietary	2029:2035	arg1	pectins					2037:2043	preferred dietary pectins	2019:2043	preferred dietary pectins	2019:2043	This knowledge can be used to define preferred dietary pectins, targeting beneficial bacteria, and favoring more balanced microbiota communities in the gut.
30828323	1	2	used	utilized	162:169	arg2	Pectins					105:111	Pectins	105:111	Pectins	105:111	Pectins are plant cell-wall polysaccharides which can be utilized by commensal bacteria in the gut, exhibiting beneficial properties for the host.
30828323	1	2	used	utilized	162:169	arg2	polysaccharides					133:147	plant cell-wall polysaccharides	117:147	plant cell-wall polysaccharides which can be utilized by commensal bacteria in the gut	117:202	Pectins are plant cell-wall polysaccharides which can be utilized by commensal bacteria in the gut, exhibiting beneficial properties for the host.
30828323	10	3	theme	pectins	1786:1792	arg1	fermentations					1751:1763	fermentations	1751:1763	fermentations of the high methoxyl pectins	1751:1792	Cumulative production of the total short chain fatty acids and propionate was largest in fermentations of the high methoxyl pectins.
30828323	9	4	theme	pectin-mediated	1437:1451	arg1	shifts					1453:1458	the pectin-mediated shifts	1433:1458	the pectin-mediated shifts in microbiota	1433:1472	The main structural features linked to the pectin-mediated shifts in microbiota included degree of esterification, composition of neutral sugars, distribution of homogalacturonan and rhamnogalacturonan fractions, degree of branching, and the presence of amide groups.
30828323	4	5	theme	citrus	660:665	arg1	fruits					667:672	citrus fruits	660:672	citrus fruits	660:672	For this purpose we performed in vitro fermentations of nine structurally diverse pectins from citrus fruits and sugar beet, and a pectic derivative, rhamnogalacturonan I (RGI), using a TIM-2 colon model.
30828323	3	6	theme	microbiota	533:542	arg1	activity					513:520	activity	513:520	activity	513:520	This study characterized the relationship between the structural properties of pectins and their potential to modulate composition and activity of the gut microbiota in a beneficial way.
30828323	3	6	theme	microbiota	533:542	arg1	composition					497:507	composition	497:507	composition	497:507	This study characterized the relationship between the structural properties of pectins and their potential to modulate composition and activity of the gut microbiota in a beneficial way.
30828323	1	7	theme	commensal	174:182	arg1	bacteria					184:191	commensal bacteria	174:191	commensal bacteria in the gut	174:202	Pectins are plant cell-wall polysaccharides which can be utilized by commensal bacteria in the gut, exhibiting beneficial properties for the host.
30828323	11	8	theme	microbial	1827:1835	arg1	communities					1837:1847	microbial communities	1827:1847	microbial communities in the gut	1827:1858	Thus, this study indicates that microbial communities in the gut can be specifically modulated by pectins and identifies the features in pectin molecules linked to microbial alterations.
30828323	2	9	theme	bacterial	301:309	arg1	communities					311:321	intestinal bacterial communities	290:321	intestinal bacterial communities	290:321	Knowledge of the impact of pectins on intestinal bacterial communities is insufficient and limited to a few types of pectins.
30828323	5	10	theme	rRNA	847:850	arg1	sequencing					866:875	rRNA gene amplicon sequencing	847:875	rRNA gene amplicon sequencing	847:875	The composition of microbiota during TIM-2 fermentations was assessed by 16S rRNA gene amplicon sequencing.
30828323	5	11	theme	TIM-2	807:811	arg1	fermentations					813:825	TIM-2 fermentations	807:825	TIM-2 fermentations	807:825	The composition of microbiota during TIM-2 fermentations was assessed by 16S rRNA gene amplicon sequencing.
30828323	6	12	theme	relative	936:943	arg1	abundances					945:954	relative abundances	936:954	relative abundances of numerous bacterial taxa	936:981	Both general and pectin-specific changes were observed in relative abundances of numerous bacterial taxa in a time-dependent way.
30828323	4	13	theme	pectins	647:653	arg1	derivative					703:712	a pectic derivative	694:712	a pectic derivative	694:712	For this purpose we performed in vitro fermentations of nine structurally diverse pectins from citrus fruits and sugar beet, and a pectic derivative, rhamnogalacturonan I (RGI), using a TIM-2 colon model.
30828323	4	13	theme	pectins	647:653	arg1	fermentations					604:616	in vitro fermentations	595:616	in vitro fermentations of nine structurally diverse pectins from citrus fruits and sugar beet	595:687	For this purpose we performed in vitro fermentations of nine structurally diverse pectins from citrus fruits and sugar beet, and a pectic derivative, rhamnogalacturonan I (RGI), using a TIM-2 colon model.
30828323	4	13	theme	pectins	647:653	arg1	I					734:734	rhamnogalacturonan I	715:734	rhamnogalacturonan I (RGI)	715:740	For this purpose we performed in vitro fermentations of nine structurally diverse pectins from citrus fruits and sugar beet, and a pectic derivative, rhamnogalacturonan I (RGI), using a TIM-2 colon model.
30828323	12	14	used	used	2004:2007	arg2	knowledge					1987:1995	This knowledge	1982:1995	This knowledge	1982:1995	This knowledge can be used to define preferred dietary pectins, targeting beneficial bacteria, and favoring more balanced microbiota communities in the gut.
30828323	4	15	theme	pectic	696:701	arg1	derivative					703:712	a pectic derivative	694:712	a pectic derivative	694:712	For this purpose we performed in vitro fermentations of nine structurally diverse pectins from citrus fruits and sugar beet, and a pectic derivative, rhamnogalacturonan I (RGI), using a TIM-2 colon model.
30828323	4	15	theme	pectic	696:701	arg1	I					734:734	rhamnogalacturonan I	715:734	rhamnogalacturonan I (RGI)	715:740	For this purpose we performed in vitro fermentations of nine structurally diverse pectins from citrus fruits and sugar beet, and a pectic derivative, rhamnogalacturonan I (RGI), using a TIM-2 colon model.
30828323	7	16	theme	human	1046:1050	arg1	health					1052:1057	human health	1046:1057	human health	1046:1057	Bacterial populations associated with human health, such as Faecalibacterium prausnitzii, Coprococcus, Ruminococcus, Dorea, Blautia, Oscillospira, Sutterella, Bifidobacterium, Christensenellaceae, Prevotella copri, and Bacteroides spp.
30828323	10	17	theme	methoxyl	1777:1784	arg1	pectins					1786:1792	the high methoxyl pectins	1768:1792	the high methoxyl pectins	1768:1792	Cumulative production of the total short chain fatty acids and propionate was largest in fermentations of the high methoxyl pectins.
30828323	1	18	theme	plant	117:121	arg1	polysaccharides					133:147	plant cell-wall polysaccharides	117:147	plant cell-wall polysaccharides which can be utilized by commensal bacteria in the gut	117:202	Pectins are plant cell-wall polysaccharides which can be utilized by commensal bacteria in the gut, exhibiting beneficial properties for the host.
30828323	1	18	theme	plant	117:121	arg1	Pectins					105:111	Pectins	105:111	Pectins	105:111	Pectins are plant cell-wall polysaccharides which can be utilized by commensal bacteria in the gut, exhibiting beneficial properties for the host.
30828323	2	19	from	impact	269:274	arg1	communities					311:321	intestinal bacterial communities	290:321	intestinal bacterial communities	290:321	Knowledge of the impact of pectins on intestinal bacterial communities is insufficient and limited to a few types of pectins.
30828323	1	20	from	bacteria	184:191	arg1	gut					200:202	the gut	196:202	the gut	196:202	Pectins are plant cell-wall polysaccharides which can be utilized by commensal bacteria in the gut, exhibiting beneficial properties for the host.
30828323	9	21	theme	sugars	1532:1537	arg1	presence					1636:1643	the presence	1632:1643	the presence of amide groups	1632:1659	The main structural features linked to the pectin-mediated shifts in microbiota included degree of esterification, composition of neutral sugars, distribution of homogalacturonan and rhamnogalacturonan fractions, degree of branching, and the presence of amide groups.
30828323	9	21	theme	sugars	1532:1537	arg1	distribution					1540:1551	distribution	1540:1551	distribution of homogalacturonan and rhamnogalacturonan fractions	1540:1604	The main structural features linked to the pectin-mediated shifts in microbiota included degree of esterification, composition of neutral sugars, distribution of homogalacturonan and rhamnogalacturonan fractions, degree of branching, and the presence of amide groups.
30828323	9	21	theme	sugars	1532:1537	arg1	composition					1509:1519	composition	1509:1519	composition of neutral sugars	1509:1537	The main structural features linked to the pectin-mediated shifts in microbiota included degree of esterification, composition of neutral sugars, distribution of homogalacturonan and rhamnogalacturonan fractions, degree of branching, and the presence of amide groups.
30828323	9	21	theme	sugars	1532:1537	arg1	degree					1607:1612	degree	1607:1612	degree of branching	1607:1625	The main structural features linked to the pectin-mediated shifts in microbiota included degree of esterification, composition of neutral sugars, distribution of homogalacturonan and rhamnogalacturonan fractions, degree of branching, and the presence of amide groups.
30828323	9	21	theme	sugars	1532:1537	arg1	degree					1483:1488	degree	1483:1488	degree of esterification	1483:1506	The main structural features linked to the pectin-mediated shifts in microbiota included degree of esterification, composition of neutral sugars, distribution of homogalacturonan and rhamnogalacturonan fractions, degree of branching, and the presence of amide groups.
30828323	1	22	theme	cell-wall	123:131	arg1	polysaccharides					133:147	plant cell-wall polysaccharides	117:147	plant cell-wall polysaccharides which can be utilized by commensal bacteria in the gut	117:202	Pectins are plant cell-wall polysaccharides which can be utilized by commensal bacteria in the gut, exhibiting beneficial properties for the host.
30828323	1	22	theme	cell-wall	123:131	arg1	Pectins					105:111	Pectins	105:111	Pectins	105:111	Pectins are plant cell-wall polysaccharides which can be utilized by commensal bacteria in the gut, exhibiting beneficial properties for the host.
30828323	11	23	theme	microbial	1959:1967	arg1	alterations					1969:1979	microbial alterations	1959:1979	microbial alterations	1959:1979	Thus, this study indicates that microbial communities in the gut can be specifically modulated by pectins and identifies the features in pectin molecules linked to microbial alterations.
30828323	7	24	theme	Bacteroides	1227:1237	arg1	spp					1239:1241	Bacteroides spp	1227:1241	Bacteroides spp	1227:1241	Bacterial populations associated with human health, such as Faecalibacterium prausnitzii, Coprococcus, Ruminococcus, Dorea, Blautia, Oscillospira, Sutterella, Bifidobacterium, Christensenellaceae, Prevotella copri, and Bacteroides spp.
30828323	5	25	theme	microbiota	789:798	arg1	composition					774:784	The composition	770:784	The composition of microbiota during TIM-2 fermentations	770:825	The composition of microbiota during TIM-2 fermentations was assessed by 16S rRNA gene amplicon sequencing.
30828323	5	26	theme	amplicon	857:864	arg1	sequencing					866:875	rRNA gene amplicon sequencing	847:875	rRNA gene amplicon sequencing	847:875	The composition of microbiota during TIM-2 fermentations was assessed by 16S rRNA gene amplicon sequencing.
30828323	6	27	theme	time-dependent	988:1001	arg1	way					1003:1005	a time-dependent way	986:1005	a time-dependent way	986:1005	Both general and pectin-specific changes were observed in relative abundances of numerous bacterial taxa in a time-dependent way.
30828323	12	28	theme	balanced	2095:2102	arg1	communities					2115:2125	more balanced microbiota communities	2090:2125	more balanced microbiota communities	2090:2125	This knowledge can be used to define preferred dietary pectins, targeting beneficial bacteria, and favoring more balanced microbiota communities in the gut.
30828323	9	29	theme	homogalacturonan	1556:1571	arg1	fractions					1596:1604	homogalacturonan and rhamnogalacturonan fractions	1556:1604	homogalacturonan and rhamnogalacturonan fractions	1556:1604	The main structural features linked to the pectin-mediated shifts in microbiota included degree of esterification, composition of neutral sugars, distribution of homogalacturonan and rhamnogalacturonan fractions, degree of branching, and the presence of amide groups.
30828323	2	30	from	communities	311:321	arg1	Knowledge					252:260	Knowledge	252:260	Knowledge of the impact of pectins on intestinal bacterial communities	252:321	Knowledge of the impact of pectins on intestinal bacterial communities is insufficient and limited to a few types of pectins.
30828323	0	31	theme	Structural	82:91	arg1	Properties					93:102	Their Structural Properties	76:102	Their Structural Properties	76:102	Potential of Pectins to Beneficially Modulate the Gut Microbiota Depends on Their Structural Properties.
30828323	9	32	theme	rhamnogalacturonan	1577:1594	arg1	fractions					1596:1604	homogalacturonan and rhamnogalacturonan fractions	1556:1604	homogalacturonan and rhamnogalacturonan fractions	1556:1604	The main structural features linked to the pectin-mediated shifts in microbiota included degree of esterification, composition of neutral sugars, distribution of homogalacturonan and rhamnogalacturonan fractions, degree of branching, and the presence of amide groups.
30828323	6	33	theme	bacterial	968:976	arg1	taxa					978:981	numerous bacterial taxa	959:981	numerous bacterial taxa	959:981	Both general and pectin-specific changes were observed in relative abundances of numerous bacterial taxa in a time-dependent way.
30828323	11	34	theme	pectin	1932:1937	arg1	molecules					1939:1947	pectin molecules	1932:1947	pectin molecules linked to microbial alterations	1932:1979	Thus, this study indicates that microbial communities in the gut can be specifically modulated by pectins and identifies the features in pectin molecules linked to microbial alterations.
30828323	8	35	theme	different	1375:1383	arg1	pectins					1385:1391	structurally different pectins	1362:1391	structurally different pectins	1362:1391	were either increased or decreased depending on the substrate, suggesting that these bacteria can be controlled using structurally different pectins.
30828323	2	36	theme	pectins	369:375	arg1	types					360:364	a few types	354:364	a few types of pectins	354:375	Knowledge of the impact of pectins on intestinal bacterial communities is insufficient and limited to a few types of pectins.
30828323	7	37	dep	copri	1216:1220	arg1	Christensenellaceae					1184:1202	Christensenellaceae	1184:1202	Christensenellaceae	1184:1202	Bacterial populations associated with human health, such as Faecalibacterium prausnitzii, Coprococcus, Ruminococcus, Dorea, Blautia, Oscillospira, Sutterella, Bifidobacterium, Christensenellaceae, Prevotella copri, and Bacteroides spp.
30828323	10	38	theme	acids	1715:1719	arg1	production					1673:1682	Cumulative production	1662:1682	Cumulative production of the total short chain fatty acids and propionate	1662:1734	Cumulative production of the total short chain fatty acids and propionate was largest in fermentations of the high methoxyl pectins.
30828323	0	39	theme	Pectins	13:19	arg1	Potential					0:8	Potential	0:8	Potential of Pectins to Beneficially Modulate the Gut Microbiota	0:63	Potential of Pectins to Beneficially Modulate the Gut Microbiota Depends on Their Structural Properties.
30828323	3	40	theme	gut	529:531	arg1	microbiota					533:542	the gut microbiota	525:542	the gut microbiota	525:542	This study characterized the relationship between the structural properties of pectins and their potential to modulate composition and activity of the gut microbiota in a beneficial way.
30828323	6	41	located	observed	924:931	arg1	way					1003:1005	a time-dependent way	986:1005	a time-dependent way	986:1005	Both general and pectin-specific changes were observed in relative abundances of numerous bacterial taxa in a time-dependent way.
30828323	6	41	located	observed	924:931	arg1	abundances					945:954	relative abundances	936:954	relative abundances of numerous bacterial taxa	936:981	Both general and pectin-specific changes were observed in relative abundances of numerous bacterial taxa in a time-dependent way.
30828323	6	41	located	observed	924:931	arg2	changes					911:917	Both general and pectin-specific changes	878:917	Both general and pectin-specific changes	878:917	Both general and pectin-specific changes were observed in relative abundances of numerous bacterial taxa in a time-dependent way.
30828323	10	42	theme	chain	1703:1707	arg1	acids					1715:1719	the total short chain fatty acids	1687:1719	the total short chain fatty acids	1687:1719	Cumulative production of the total short chain fatty acids and propionate was largest in fermentations of the high methoxyl pectins.
30828323	3	43	theme	structural	432:441	arg1	properties					443:452	the structural properties	428:452	the structural properties of pectins	428:463	This study characterized the relationship between the structural properties of pectins and their potential to modulate composition and activity of the gut microbiota in a beneficial way.
30828323	4	44	theme	colon	757:761	arg1	model					763:767	a TIM-2 colon model	749:767	a TIM-2 colon model	749:767	For this purpose we performed in vitro fermentations of nine structurally diverse pectins from citrus fruits and sugar beet, and a pectic derivative, rhamnogalacturonan I (RGI), using a TIM-2 colon model.
30828323	10	45	theme	propionate	1725:1734	arg1	production					1673:1682	Cumulative production	1662:1682	Cumulative production of the total short chain fatty acids and propionate	1662:1734	Cumulative production of the total short chain fatty acids and propionate was largest in fermentations of the high methoxyl pectins.
30828323	4	46	from	derivative	703:712	arg1	fruits					667:672	citrus fruits	660:672	citrus fruits	660:672	For this purpose we performed in vitro fermentations of nine structurally diverse pectins from citrus fruits and sugar beet, and a pectic derivative, rhamnogalacturonan I (RGI), using a TIM-2 colon model.
30828323	4	46	from	derivative	703:712	arg1	beet					684:687	sugar beet	678:687	sugar beet	678:687	For this purpose we performed in vitro fermentations of nine structurally diverse pectins from citrus fruits and sugar beet, and a pectic derivative, rhamnogalacturonan I (RGI), using a TIM-2 colon model.
30828323	1	47	theme	beneficial	216:225	arg1	properties					227:236	beneficial properties	216:236	beneficial properties for the host	216:249	Pectins are plant cell-wall polysaccharides which can be utilized by commensal bacteria in the gut, exhibiting beneficial properties for the host.
30828323	10	48	theme	high	1772:1775	arg1	pectins					1786:1792	the high methoxyl pectins	1768:1792	the high methoxyl pectins	1768:1792	Cumulative production of the total short chain fatty acids and propionate was largest in fermentations of the high methoxyl pectins.
30828323	9	49	theme	groups	1654:1659	arg1	presence					1636:1643	the presence	1632:1643	the presence of amide groups	1632:1659	The main structural features linked to the pectin-mediated shifts in microbiota included degree of esterification, composition of neutral sugars, distribution of homogalacturonan and rhamnogalacturonan fractions, degree of branching, and the presence of amide groups.
30828323	9	49	theme	groups	1654:1659	arg1	distribution					1540:1551	distribution	1540:1551	distribution of homogalacturonan and rhamnogalacturonan fractions	1540:1604	The main structural features linked to the pectin-mediated shifts in microbiota included degree of esterification, composition of neutral sugars, distribution of homogalacturonan and rhamnogalacturonan fractions, degree of branching, and the presence of amide groups.
30828323	9	49	theme	groups	1654:1659	arg1	composition					1509:1519	composition	1509:1519	composition of neutral sugars	1509:1537	The main structural features linked to the pectin-mediated shifts in microbiota included degree of esterification, composition of neutral sugars, distribution of homogalacturonan and rhamnogalacturonan fractions, degree of branching, and the presence of amide groups.
30828323	9	49	theme	groups	1654:1659	arg1	degree					1607:1612	degree	1607:1612	degree of branching	1607:1625	The main structural features linked to the pectin-mediated shifts in microbiota included degree of esterification, composition of neutral sugars, distribution of homogalacturonan and rhamnogalacturonan fractions, degree of branching, and the presence of amide groups.
30828323	9	49	theme	groups	1654:1659	arg1	degree					1483:1488	degree	1483:1488	degree of esterification	1483:1506	The main structural features linked to the pectin-mediated shifts in microbiota included degree of esterification, composition of neutral sugars, distribution of homogalacturonan and rhamnogalacturonan fractions, degree of branching, and the presence of amide groups.
30828323	4	50	theme	in	595:596	arg1	fermentations					604:616	in vitro fermentations	595:616	in vitro fermentations of nine structurally diverse pectins from citrus fruits and sugar beet	595:687	For this purpose we performed in vitro fermentations of nine structurally diverse pectins from citrus fruits and sugar beet, and a pectic derivative, rhamnogalacturonan I (RGI), using a TIM-2 colon model.
30828323	3	51	theme	pectins	457:463	arg1	potential					475:483	their potential to modulate composition and activity of the gut microbiota in a beneficial way	469:562	their potential to modulate composition and activity of the gut microbiota in a beneficial way	469:562	This study characterized the relationship between the structural properties of pectins and their potential to modulate composition and activity of the gut microbiota in a beneficial way.
30828323	3	51	theme	pectins	457:463	arg1	properties					443:452	the structural properties	428:452	the structural properties of pectins	428:463	This study characterized the relationship between the structural properties of pectins and their potential to modulate composition and activity of the gut microbiota in a beneficial way.
30828323	9	52	theme	structural	1403:1412	arg1	features					1414:1421	The main structural features	1394:1421	The main structural features linked to the pectin-mediated shifts in microbiota	1394:1472	The main structural features linked to the pectin-mediated shifts in microbiota included degree of esterification, composition of neutral sugars, distribution of homogalacturonan and rhamnogalacturonan fractions, degree of branching, and the presence of amide groups.
30828323	6	53	theme	pectin-specific	895:909	arg1	changes					911:917	Both general and pectin-specific changes	878:917	Both general and pectin-specific changes	878:917	Both general and pectin-specific changes were observed in relative abundances of numerous bacterial taxa in a time-dependent way.
30828323	9	54	from	shifts	1453:1458	arg1	microbiota					1463:1472	microbiota	1463:1472	microbiota	1463:1472	The main structural features linked to the pectin-mediated shifts in microbiota included degree of esterification, composition of neutral sugars, distribution of homogalacturonan and rhamnogalacturonan fractions, degree of branching, and the presence of amide groups.
30828323	6	55	theme	general	883:889	arg1	changes					911:917	Both general and pectin-specific changes	878:917	Both general and pectin-specific changes	878:917	Both general and pectin-specific changes were observed in relative abundances of numerous bacterial taxa in a time-dependent way.
30828323	2	56	theme	pectins	279:285	arg1	impact					269:274	the impact	265:274	the impact of pectins on intestinal bacterial communities	265:321	Knowledge of the impact of pectins on intestinal bacterial communities is insufficient and limited to a few types of pectins.
30828323	12	57	theme	preferred	2019:2027	arg1	pectins					2037:2043	preferred dietary pectins	2019:2043	preferred dietary pectins	2019:2043	This knowledge can be used to define preferred dietary pectins, targeting beneficial bacteria, and favoring more balanced microbiota communities in the gut.
30828323	2	58	theme	impact	269:274	arg1	Knowledge					252:260	Knowledge	252:260	Knowledge of the impact of pectins on intestinal bacterial communities	252:321	Knowledge of the impact of pectins on intestinal bacterial communities is insufficient and limited to a few types of pectins.
30828323	4	59	from	fruits	667:672	arg1	derivative					703:712	a pectic derivative	694:712	a pectic derivative	694:712	For this purpose we performed in vitro fermentations of nine structurally diverse pectins from citrus fruits and sugar beet, and a pectic derivative, rhamnogalacturonan I (RGI), using a TIM-2 colon model.
30828323	4	59	from	fruits	667:672	arg1	pectins					647:653	nine structurally diverse pectins	621:653	nine structurally diverse pectins from citrus fruits and sugar beet	621:687	For this purpose we performed in vitro fermentations of nine structurally diverse pectins from citrus fruits and sugar beet, and a pectic derivative, rhamnogalacturonan I (RGI), using a TIM-2 colon model.
30828323	4	59	from	fruits	667:672	arg1	fermentations					604:616	in vitro fermentations	595:616	in vitro fermentations of nine structurally diverse pectins from citrus fruits and sugar beet	595:687	For this purpose we performed in vitro fermentations of nine structurally diverse pectins from citrus fruits and sugar beet, and a pectic derivative, rhamnogalacturonan I (RGI), using a TIM-2 colon model.
30828323	4	59	from	fruits	667:672	arg1	I					734:734	rhamnogalacturonan I	715:734	rhamnogalacturonan I (RGI)	715:740	For this purpose we performed in vitro fermentations of nine structurally diverse pectins from citrus fruits and sugar beet, and a pectic derivative, rhamnogalacturonan I (RGI), using a TIM-2 colon model.
30828323	9	60	attach	linked	1423:1428	arg1	shifts					1453:1458	the pectin-mediated shifts	1433:1458	the pectin-mediated shifts in microbiota	1433:1472	The main structural features linked to the pectin-mediated shifts in microbiota included degree of esterification, composition of neutral sugars, distribution of homogalacturonan and rhamnogalacturonan fractions, degree of branching, and the presence of amide groups.
30828323	9	60	attach	linked	1423:1428	arg2	features					1414:1421	The main structural features	1394:1421	The main structural features linked to the pectin-mediated shifts in microbiota	1394:1472	The main structural features linked to the pectin-mediated shifts in microbiota included degree of esterification, composition of neutral sugars, distribution of homogalacturonan and rhamnogalacturonan fractions, degree of branching, and the presence of amide groups.
30828323	6	61	theme	numerous	959:966	arg1	taxa					978:981	numerous bacterial taxa	959:981	numerous bacterial taxa	959:981	Both general and pectin-specific changes were observed in relative abundances of numerous bacterial taxa in a time-dependent way.
30828323	11	62	from	communities	1837:1847	arg1	gut					1856:1858	the gut	1852:1858	the gut	1852:1858	Thus, this study indicates that microbial communities in the gut can be specifically modulated by pectins and identifies the features in pectin molecules linked to microbial alterations.
30828323	2	63	theme	intestinal	290:299	arg1	communities					311:321	intestinal bacterial communities	290:321	intestinal bacterial communities	290:321	Knowledge of the impact of pectins on intestinal bacterial communities is insufficient and limited to a few types of pectins.
30828323	10	64	theme	Cumulative	1662:1671	arg1	production					1673:1682	Cumulative production	1662:1682	Cumulative production of the total short chain fatty acids and propionate	1662:1734	Cumulative production of the total short chain fatty acids and propionate was largest in fermentations of the high methoxyl pectins.
30828323	9	65	theme	esterification	1493:1506	arg1	presence					1636:1643	the presence	1632:1643	the presence of amide groups	1632:1659	The main structural features linked to the pectin-mediated shifts in microbiota included degree of esterification, composition of neutral sugars, distribution of homogalacturonan and rhamnogalacturonan fractions, degree of branching, and the presence of amide groups.
30828323	9	65	theme	esterification	1493:1506	arg1	distribution					1540:1551	distribution	1540:1551	distribution of homogalacturonan and rhamnogalacturonan fractions	1540:1604	The main structural features linked to the pectin-mediated shifts in microbiota included degree of esterification, composition of neutral sugars, distribution of homogalacturonan and rhamnogalacturonan fractions, degree of branching, and the presence of amide groups.
30828323	9	65	theme	esterification	1493:1506	arg1	composition					1509:1519	composition	1509:1519	composition of neutral sugars	1509:1537	The main structural features linked to the pectin-mediated shifts in microbiota included degree of esterification, composition of neutral sugars, distribution of homogalacturonan and rhamnogalacturonan fractions, degree of branching, and the presence of amide groups.
30828323	9	65	theme	esterification	1493:1506	arg1	degree					1607:1612	degree	1607:1612	degree of branching	1607:1625	The main structural features linked to the pectin-mediated shifts in microbiota included degree of esterification, composition of neutral sugars, distribution of homogalacturonan and rhamnogalacturonan fractions, degree of branching, and the presence of amide groups.
30828323	9	65	theme	esterification	1493:1506	arg1	degree					1483:1488	degree	1483:1488	degree of esterification	1483:1506	The main structural features linked to the pectin-mediated shifts in microbiota included degree of esterification, composition of neutral sugars, distribution of homogalacturonan and rhamnogalacturonan fractions, degree of branching, and the presence of amide groups.
30828323	4	66	theme	diverse	639:645	arg1	pectins					647:653	nine structurally diverse pectins	621:653	nine structurally diverse pectins from citrus fruits and sugar beet	621:687	For this purpose we performed in vitro fermentations of nine structurally diverse pectins from citrus fruits and sugar beet, and a pectic derivative, rhamnogalacturonan I (RGI), using a TIM-2 colon model.
30828323	7	67	theme	Bacterial	1008:1016	arg1	populations					1018:1028	Bacterial populations	1008:1028	Bacterial populations	1008:1028	Bacterial populations associated with human health, such as Faecalibacterium prausnitzii, Coprococcus, Ruminococcus, Dorea, Blautia, Oscillospira, Sutterella, Bifidobacterium, Christensenellaceae, Prevotella copri, and Bacteroides spp.
30828323	12	68	theme	microbiota	2104:2113	arg1	communities					2115:2125	more balanced microbiota communities	2090:2125	more balanced microbiota communities	2090:2125	This knowledge can be used to define preferred dietary pectins, targeting beneficial bacteria, and favoring more balanced microbiota communities in the gut.
30828323	9	69	theme	neutral	1524:1530	arg1	sugars					1532:1537	neutral sugars	1524:1537	neutral sugars	1524:1537	The main structural features linked to the pectin-mediated shifts in microbiota included degree of esterification, composition of neutral sugars, distribution of homogalacturonan and rhamnogalacturonan fractions, degree of branching, and the presence of amide groups.
30828323	3	70	theme	beneficial	549:558	arg1	way					560:562	a beneficial way	547:562	a beneficial way	547:562	This study characterized the relationship between the structural properties of pectins and their potential to modulate composition and activity of the gut microbiota in a beneficial way.
30828323	11	71	from	features	1920:1927	arg1	molecules					1939:1947	pectin molecules	1932:1947	pectin molecules linked to microbial alterations	1932:1979	Thus, this study indicates that microbial communities in the gut can be specifically modulated by pectins and identifies the features in pectin molecules linked to microbial alterations.
30828323	4	72	dep	in	595:596	arg1	vitro					598:602	vitro	598:602	vitro	598:602	For this purpose we performed in vitro fermentations of nine structurally diverse pectins from citrus fruits and sugar beet, and a pectic derivative, rhamnogalacturonan I (RGI), using a TIM-2 colon model.
30828323	5	73	theme	gene	852:855	arg1	sequencing					866:875	rRNA gene amplicon sequencing	847:875	rRNA gene amplicon sequencing	847:875	The composition of microbiota during TIM-2 fermentations was assessed by 16S rRNA gene amplicon sequencing.
30828323	4	74	theme	sugar	678:682	arg1	beet					684:687	sugar beet	678:687	sugar beet	678:687	For this purpose we performed in vitro fermentations of nine structurally diverse pectins from citrus fruits and sugar beet, and a pectic derivative, rhamnogalacturonan I (RGI), using a TIM-2 colon model.
30828323	6	75	theme	taxa	978:981	arg1	abundances					945:954	relative abundances	936:954	relative abundances of numerous bacterial taxa	936:981	Both general and pectin-specific changes were observed in relative abundances of numerous bacterial taxa in a time-dependent way.
30828323	9	76	theme	fractions	1596:1604	arg1	presence					1636:1643	the presence	1632:1643	the presence of amide groups	1632:1659	The main structural features linked to the pectin-mediated shifts in microbiota included degree of esterification, composition of neutral sugars, distribution of homogalacturonan and rhamnogalacturonan fractions, degree of branching, and the presence of amide groups.
30828323	9	76	theme	fractions	1596:1604	arg1	distribution					1540:1551	distribution	1540:1551	distribution of homogalacturonan and rhamnogalacturonan fractions	1540:1604	The main structural features linked to the pectin-mediated shifts in microbiota included degree of esterification, composition of neutral sugars, distribution of homogalacturonan and rhamnogalacturonan fractions, degree of branching, and the presence of amide groups.
30828323	9	76	theme	fractions	1596:1604	arg1	composition					1509:1519	composition	1509:1519	composition of neutral sugars	1509:1537	The main structural features linked to the pectin-mediated shifts in microbiota included degree of esterification, composition of neutral sugars, distribution of homogalacturonan and rhamnogalacturonan fractions, degree of branching, and the presence of amide groups.
30828323	9	76	theme	fractions	1596:1604	arg1	degree					1607:1612	degree	1607:1612	degree of branching	1607:1625	The main structural features linked to the pectin-mediated shifts in microbiota included degree of esterification, composition of neutral sugars, distribution of homogalacturonan and rhamnogalacturonan fractions, degree of branching, and the presence of amide groups.
30828323	9	76	theme	fractions	1596:1604	arg1	degree					1483:1488	degree	1483:1488	degree of esterification	1483:1506	The main structural features linked to the pectin-mediated shifts in microbiota included degree of esterification, composition of neutral sugars, distribution of homogalacturonan and rhamnogalacturonan fractions, degree of branching, and the presence of amide groups.
30828323	4	77	theme	rhamnogalacturonan	715:732	arg1	RGI					737:739	RGI	737:739	RGI	737:739	For this purpose we performed in vitro fermentations of nine structurally diverse pectins from citrus fruits and sugar beet, and a pectic derivative, rhamnogalacturonan I (RGI), using a TIM-2 colon model.
30828323	4	77	theme	rhamnogalacturonan	715:732	arg1	derivative					703:712	a pectic derivative	694:712	a pectic derivative	694:712	For this purpose we performed in vitro fermentations of nine structurally diverse pectins from citrus fruits and sugar beet, and a pectic derivative, rhamnogalacturonan I (RGI), using a TIM-2 colon model.
30828323	4	77	theme	rhamnogalacturonan	715:732	arg1	I					734:734	rhamnogalacturonan I	715:734	rhamnogalacturonan I (RGI)	715:740	For this purpose we performed in vitro fermentations of nine structurally diverse pectins from citrus fruits and sugar beet, and a pectic derivative, rhamnogalacturonan I (RGI), using a TIM-2 colon model.
30828323	9	78	theme	branching	1617:1625	arg1	presence					1636:1643	the presence	1632:1643	the presence of amide groups	1632:1659	The main structural features linked to the pectin-mediated shifts in microbiota included degree of esterification, composition of neutral sugars, distribution of homogalacturonan and rhamnogalacturonan fractions, degree of branching, and the presence of amide groups.
30828323	9	78	theme	branching	1617:1625	arg1	distribution					1540:1551	distribution	1540:1551	distribution of homogalacturonan and rhamnogalacturonan fractions	1540:1604	The main structural features linked to the pectin-mediated shifts in microbiota included degree of esterification, composition of neutral sugars, distribution of homogalacturonan and rhamnogalacturonan fractions, degree of branching, and the presence of amide groups.
30828323	9	78	theme	branching	1617:1625	arg1	composition					1509:1519	composition	1509:1519	composition of neutral sugars	1509:1537	The main structural features linked to the pectin-mediated shifts in microbiota included degree of esterification, composition of neutral sugars, distribution of homogalacturonan and rhamnogalacturonan fractions, degree of branching, and the presence of amide groups.
30828323	9	78	theme	branching	1617:1625	arg1	degree					1607:1612	degree	1607:1612	degree of branching	1607:1625	The main structural features linked to the pectin-mediated shifts in microbiota included degree of esterification, composition of neutral sugars, distribution of homogalacturonan and rhamnogalacturonan fractions, degree of branching, and the presence of amide groups.
30828323	9	78	theme	branching	1617:1625	arg1	degree					1483:1488	degree	1483:1488	degree of esterification	1483:1506	The main structural features linked to the pectin-mediated shifts in microbiota included degree of esterification, composition of neutral sugars, distribution of homogalacturonan and rhamnogalacturonan fractions, degree of branching, and the presence of amide groups.
30828323	10	79	theme	fatty	1709:1713	arg1	acids					1715:1719	the total short chain fatty acids	1687:1719	the total short chain fatty acids	1687:1719	Cumulative production of the total short chain fatty acids and propionate was largest in fermentations of the high methoxyl pectins.
30828323	2	80	from	Knowledge	252:260	arg1	communities					311:321	intestinal bacterial communities	290:321	intestinal bacterial communities	290:321	Knowledge of the impact of pectins on intestinal bacterial communities is insufficient and limited to a few types of pectins.
30828323	10	81	theme	total	1691:1695	arg1	acids					1715:1719	the total short chain fatty acids	1687:1719	the total short chain fatty acids	1687:1719	Cumulative production of the total short chain fatty acids and propionate was largest in fermentations of the high methoxyl pectins.
30828323	10	82	theme	short	1697:1701	arg1	acids					1715:1719	the total short chain fatty acids	1687:1719	the total short chain fatty acids	1687:1719	Cumulative production of the total short chain fatty acids and propionate was largest in fermentations of the high methoxyl pectins.
30828323	0	83	theme	Gut	50:52	arg1	Microbiota					54:63	the Gut Microbiota	46:63	the Gut Microbiota	46:63	Potential of Pectins to Beneficially Modulate the Gut Microbiota Depends on Their Structural Properties.
30828323	11	84	attach	linked	1949:1954	arg2	molecules					1939:1947	pectin molecules	1932:1947	pectin molecules linked to microbial alterations	1932:1979	Thus, this study indicates that microbial communities in the gut can be specifically modulated by pectins and identifies the features in pectin molecules linked to microbial alterations.
30828323	11	84	attach	linked	1949:1954	arg1	alterations					1969:1979	microbial alterations	1959:1979	microbial alterations	1959:1979	Thus, this study indicates that microbial communities in the gut can be specifically modulated by pectins and identifies the features in pectin molecules linked to microbial alterations.
30828323	9	85	theme	amide	1648:1652	arg1	groups					1654:1659	amide groups	1648:1659	amide groups	1648:1659	The main structural features linked to the pectin-mediated shifts in microbiota included degree of esterification, composition of neutral sugars, distribution of homogalacturonan and rhamnogalacturonan fractions, degree of branching, and the presence of amide groups.
30828323	4	86	from	fermentations	604:616	arg1	fruits					667:672	citrus fruits	660:672	citrus fruits	660:672	For this purpose we performed in vitro fermentations of nine structurally diverse pectins from citrus fruits and sugar beet, and a pectic derivative, rhamnogalacturonan I (RGI), using a TIM-2 colon model.
30828323	4	86	from	fermentations	604:616	arg1	beet					684:687	sugar beet	678:687	sugar beet	678:687	For this purpose we performed in vitro fermentations of nine structurally diverse pectins from citrus fruits and sugar beet, and a pectic derivative, rhamnogalacturonan I (RGI), using a TIM-2 colon model.
30828323	4	87	theme	TIM-2	751:755	arg1	model					763:767	a TIM-2 colon model	749:767	a TIM-2 colon model	749:767	For this purpose we performed in vitro fermentations of nine structurally diverse pectins from citrus fruits and sugar beet, and a pectic derivative, rhamnogalacturonan I (RGI), using a TIM-2 colon model.
30828323	9	88	theme	main	1398:1401	arg1	features					1414:1421	The main structural features	1394:1421	The main structural features linked to the pectin-mediated shifts in microbiota	1394:1472	The main structural features linked to the pectin-mediated shifts in microbiota included degree of esterification, composition of neutral sugars, distribution of homogalacturonan and rhamnogalacturonan fractions, degree of branching, and the presence of amide groups.
30828323	12	89	theme	beneficial	2056:2065	arg1	bacteria					2067:2074	beneficial bacteria	2056:2074	beneficial bacteria	2056:2074	This knowledge can be used to define preferred dietary pectins, targeting beneficial bacteria, and favoring more balanced microbiota communities in the gut.
30828323	2	90	theme	few	356:358	arg1	types					360:364	a few types	354:364	a few types of pectins	354:375	Knowledge of the impact of pectins on intestinal bacterial communities is insufficient and limited to a few types of pectins.
30305844	8	0	with	relationship	1373:1384	arg1	saccharification					1391:1406	saccharification	1391:1406	saccharification	1391:1406	Testing the relationship with saccharification demonstrated that accessibility of 1.3-1.7-nm radius probes measured by FRAP on non-hydrolysed samples was highly correlated with poplar digestibility together with the measurement of initial porosity on the range 5-20 nm.
30305844	4	1	theme	polyethylene	735:746	arg1	glycol					748:753	polyethylene glycol	735:753	polyethylene glycol probes of different sizes	735:779	In addition to chemical composition and porosity analyses, the diffusion of polyethylene glycol probes of different sizes was measured at three different time points during the saccharification.
30305844	8	2	theme	porosity	1600:1607	arg1	measurement					1577:1587	the measurement	1573:1587	the measurement of initial porosity on the range 5-20 nm	1573:1628	Testing the relationship with saccharification demonstrated that accessibility of 1.3-1.7-nm radius probes measured by FRAP on non-hydrolysed samples was highly correlated with poplar digestibility together with the measurement of initial porosity on the range 5-20 nm.
30305844	6	3	theme	lignin	1136:1141	arg1	conformation					1143:1154	lignin conformation	1136:1154	lignin conformation	1136:1154	This means that, despite the removal of polysaccharides during saccharification, diffusion of probes was not improved since they became hindered by changes in lignin conformation, whose relative amount increased over time.
30305844	8	4	from	accessibility	1426:1438	arg1	samples					1503:1509	non-hydrolysed samples	1488:1509	non-hydrolysed samples	1488:1509	Testing the relationship with saccharification demonstrated that accessibility of 1.3-1.7-nm radius probes measured by FRAP on non-hydrolysed samples was highly correlated with poplar digestibility together with the measurement of initial porosity on the range 5-20 nm.
30305844	8	5	from	measurement	1577:1587	arg1	5-20 nm					1622:1628	the range 5-20 nm	1612:1628	the range 5-20 nm	1612:1628	Testing the relationship with saccharification demonstrated that accessibility of 1.3-1.7-nm radius probes measured by FRAP on non-hydrolysed samples was highly correlated with poplar digestibility together with the measurement of initial porosity on the range 5-20 nm.
30305844	4	6	dep	analyses	708:715	arg1	addition					662:669	addition	662:669	addition	662:669	In addition to chemical composition and porosity analyses, the diffusion of polyethylene glycol probes of different sizes was measured at three different time points during the saccharification.
30305844	2	7	theme	chemical	316:323	arg1	detrimental					352:362	detrimental	352:362	detrimental	352:362	Although many chemical features are considered as detrimental to saccharification, enzymes' dynamics within the cell walls remains poorly explored and understood.
30305844	2	7	theme	chemical	316:323	arg1	features					325:332	many chemical features	311:332	many chemical features	311:332	Although many chemical features are considered as detrimental to saccharification, enzymes' dynamics within the cell walls remains poorly explored and understood.
30305844	9	8	theme	CONCLUSION	1631:1640	arg1	measurements					1651:1662	CONCLUSION Mobility measurements	1631:1662	CONCLUSION Mobility measurements performed before hydrolysis	1631:1690	CONCLUSION Mobility measurements performed before hydrolysis can serve to explain and even predict saccharification with accuracy.
30305844	2	9	theme	many	311:314	arg1	detrimental					352:362	detrimental	352:362	detrimental	352:362	Although many chemical features are considered as detrimental to saccharification, enzymes' dynamics within the cell walls remains poorly explored and understood.
30305844	2	9	theme	many	311:314	arg1	features					325:332	many chemical features	311:332	many chemical features	311:332	Although many chemical features are considered as detrimental to saccharification, enzymes' dynamics within the cell walls remains poorly explored and understood.
30305844	5	10	theme	saccharification	954:969	arg1	time					971:974	saccharification time	954:974	saccharification time	954:974	RESULTS Probes' diffusion was mainly affected by probes size and pretreatments but only slightly by saccharification time.
30305844	6	11	from	changes	1125:1131	arg1	conformation					1143:1154	lignin conformation	1136:1154	lignin conformation	1136:1154	This means that, despite the removal of polysaccharides during saccharification, diffusion of probes was not improved since they became hindered by changes in lignin conformation, whose relative amount increased over time.
30305844	8	12	theme	poplar	1538:1543	arg1	digestibility					1545:1557	poplar digestibility	1538:1557	poplar digestibility together with the measurement of initial porosity on the range 5-20 nm	1538:1628	Testing the relationship with saccharification demonstrated that accessibility of 1.3-1.7-nm radius probes measured by FRAP on non-hydrolysed samples was highly correlated with poplar digestibility together with the measurement of initial porosity on the range 5-20 nm.
30305844	7	13	theme	probes	1353:1358	arg1	size					1341:1344	the size	1337:1344	least five times the size of the probes	1320:1358	Porosity measurements showed that probes' diffusion was highly correlated with the amount of pores having a diameter at least five times the size of the probes.
30305844	4	14	theme	porosity	699:706	arg1	analyses					708:715	chemical composition and porosity analyses	674:715	chemical composition and porosity analyses	674:715	In addition to chemical composition and porosity analyses, the diffusion of polyethylene glycol probes of different sizes was measured at three different time points during the saccharification.
30305844	0	15	from	mobility	43:50	arg1	walls					67:71	poplar cell walls	55:71	poplar cell walls	55:71	Dynamical assessment of fluorescent probes mobility in poplar cell walls reveals nanopores govern saccharification.
30305844	4	16	theme	time	813:816	arg1	points					818:823	three different time points	797:823	three different time points during the saccharification	797:851	In addition to chemical composition and porosity analyses, the diffusion of polyethylene glycol probes of different sizes was measured at three different time points during the saccharification.
30305844	3	17	dep	structure	614:622	arg1	the					610:612	the	610:612	the	610:612	To address this issue, poplar fragments were submitted to hot water and ionic liquid pretreatments selected for their contrasted effects on both the structure and composition of lignocellulose.
30305844	5	18	theme	RESULTS	854:860	arg1	Probes					862:867	RESULTS Probes'	854:868	RESULTS Probes' diffusion	854:878	RESULTS Probes' diffusion was mainly affected by probes size and pretreatments but only slightly by saccharification time.
30305844	8	19	theme	range	1616:1620	arg1	5-20 nm					1622:1628	the range 5-20 nm	1612:1628	the range 5-20 nm	1612:1628	Testing the relationship with saccharification demonstrated that accessibility of 1.3-1.7-nm radius probes measured by FRAP on non-hydrolysed samples was highly correlated with poplar digestibility together with the measurement of initial porosity on the range 5-20 nm.
30305844	2	20	theme	cell	414:417	arg1	walls					419:423	the cell walls	410:423	the cell walls	410:423	Although many chemical features are considered as detrimental to saccharification, enzymes' dynamics within the cell walls remains poorly explored and understood.
30305844	0	21	theme	Dynamical	0:8	arg1	assessment					10:19	Dynamical assessment	0:19	Dynamical assessment of fluorescent probes mobility in poplar cell walls	0:71	Dynamical assessment of fluorescent probes mobility in poplar cell walls reveals nanopores govern saccharification.
30305844	6	22	theme	polysaccharides	1017:1031	arg1	removal					1006:1012	the removal	1002:1012	the removal of polysaccharides during saccharification	1002:1055	This means that, despite the removal of polysaccharides during saccharification, diffusion of probes was not improved since they became hindered by changes in lignin conformation, whose relative amount increased over time.
30305844	0	23	dep	reveals	73:79	arg1	govern					91:96	govern	91:96	reveals nanopores govern saccharification	73:113	Dynamical assessment of fluorescent probes mobility in poplar cell walls reveals nanopores govern saccharification.
30305844	0	24	theme	fluorescent	24:34	arg1	probes					36:41	fluorescent probes	24:41	fluorescent probes mobility in poplar cell walls	24:71	Dynamical assessment of fluorescent probes mobility in poplar cell walls reveals nanopores govern saccharification.
30305844	10	25	theme	lignin	1896:1901	arg1	content					1903:1909	lignin content	1896:1909	lignin content	1896:1909	The discrepancy observed between probes' size and pores' diameters to explain accessibility is likely due to biomass features such as lignin content and composition that prevent probes' diffusion through non-specific interactions probably leading to pores' entanglements.
30305844	3	26	theme	contrasted	583:592	arg1	effects					594:600	their contrasted effects	577:600	their contrasted effects on both the structure and composition of lignocellulose	577:656	To address this issue, poplar fragments were submitted to hot water and ionic liquid pretreatments selected for their contrasted effects on both the structure and composition of lignocellulose.
30305844	3	27	theme	poplar	488:493	arg1	fragments					495:503	poplar fragments	488:503	poplar fragments	488:503	To address this issue, poplar fragments were submitted to hot water and ionic liquid pretreatments selected for their contrasted effects on both the structure and composition of lignocellulose.
30305844	4	28	theme	sizes	775:779	arg1	probes					755:760	polyethylene glycol probes	735:760	polyethylene glycol probes of different sizes	735:779	In addition to chemical composition and porosity analyses, the diffusion of polyethylene glycol probes of different sizes was measured at three different time points during the saccharification.
30305844	10	29	dep	size	1803:1806	arg1	explain					1832:1838	explain	1832:1838	to explain accessibility	1829:1852	The discrepancy observed between probes' size and pores' diameters to explain accessibility is likely due to biomass features such as lignin content and composition that prevent probes' diffusion through non-specific interactions probably leading to pores' entanglements.
30305844	9	30	theme	Mobility	1642:1649	arg1	measurements					1651:1662	CONCLUSION Mobility measurements	1631:1662	CONCLUSION Mobility measurements performed before hydrolysis	1631:1690	CONCLUSION Mobility measurements performed before hydrolysis can serve to explain and even predict saccharification with accuracy.
30305844	10	31	theme	biomass	1871:1877	arg1	features					1879:1886	biomass features	1871:1886	biomass features such as lignin content and composition that prevent probes' diffusion through non-specific interactions probably leading to pores' entanglements	1871:2031	The discrepancy observed between probes' size and pores' diameters to explain accessibility is likely due to biomass features such as lignin content and composition that prevent probes' diffusion through non-specific interactions probably leading to pores' entanglements.
30305844	10	31	theme	biomass	1871:1877	arg1	composition					1915:1925	composition	1915:1925	composition	1915:1925	The discrepancy observed between probes' size and pores' diameters to explain accessibility is likely due to biomass features such as lignin content and composition that prevent probes' diffusion through non-specific interactions probably leading to pores' entanglements.
30305844	10	31	theme	biomass	1871:1877	arg1	content					1903:1909	lignin content	1896:1909	lignin content	1896:1909	The discrepancy observed between probes' size and pores' diameters to explain accessibility is likely due to biomass features such as lignin content and composition that prevent probes' diffusion through non-specific interactions probably leading to pores' entanglements.
30305844	1	32	theme	cell	224:227	arg1	walls					229:233	the plant cell walls	214:233	the plant cell walls	214:233	BACKGROUND Improving lignocellulolytic enzymes' diffusion and accessibility to their substrate in the plant cell walls is recognised as a critical issue for optimising saccharification.
30305844	7	33	theme	Porosity	1200:1207	arg1	measurements					1209:1220	Porosity measurements	1200:1220	Porosity measurements	1200:1220	Porosity measurements showed that probes' diffusion was highly correlated with the amount of pores having a diameter at least five times the size of the probes.
30305844	3	34	theme	hot	523:525	arg1	water					527:531	hot water	523:531	hot water	523:531	To address this issue, poplar fragments were submitted to hot water and ionic liquid pretreatments selected for their contrasted effects on both the structure and composition of lignocellulose.
30305844	10	35	theme	non-specific	1966:1977	arg1	interactions					1979:1990	non-specific interactions	1966:1990	non-specific interactions probably leading to pores' entanglements	1966:2031	The discrepancy observed between probes' size and pores' diameters to explain accessibility is likely due to biomass features such as lignin content and composition that prevent probes' diffusion through non-specific interactions probably leading to pores' entanglements.
30305844	4	36	theme	composition	683:693	arg1	analyses					708:715	chemical composition and porosity analyses	674:715	chemical composition and porosity analyses	674:715	In addition to chemical composition and porosity analyses, the diffusion of polyethylene glycol probes of different sizes was measured at three different time points during the saccharification.
30305844	0	37	theme	probes	36:41	arg1	assessment					10:19	Dynamical assessment	0:19	Dynamical assessment of fluorescent probes mobility in poplar cell walls	0:71	Dynamical assessment of fluorescent probes mobility in poplar cell walls reveals nanopores govern saccharification.
30305844	4	38	theme	glycol	748:753	arg1	probes					755:760	polyethylene glycol probes	735:760	polyethylene glycol probes of different sizes	735:779	In addition to chemical composition and porosity analyses, the diffusion of polyethylene glycol probes of different sizes was measured at three different time points during the saccharification.
30305844	8	39	theme	radius probes	1454:1466	arg1	accessibility					1426:1438	accessibility	1426:1438	accessibility of 1.3-1.7-nm radius probes measured by FRAP on non-hydrolysed samples	1426:1509	Testing the relationship with saccharification demonstrated that accessibility of 1.3-1.7-nm radius probes measured by FRAP on non-hydrolysed samples was highly correlated with poplar digestibility together with the measurement of initial porosity on the range 5-20 nm.
30305844	4	40	theme	chemical	674:681	arg1	composition					683:693	chemical composition	674:693	chemical composition	674:693	In addition to chemical composition and porosity analyses, the diffusion of polyethylene glycol probes of different sizes was measured at three different time points during the saccharification.
30305844	0	41	theme	poplar	55:60	arg1	walls					67:71	poplar cell walls	55:71	poplar cell walls	55:71	Dynamical assessment of fluorescent probes mobility in poplar cell walls reveals nanopores govern saccharification.
30305844	5	42	dep	probes	903:908	arg1	size					910:913	size	910:913	size	910:913	RESULTS Probes' diffusion was mainly affected by probes size and pretreatments but only slightly by saccharification time.
30305844	5	42	dep	probes	903:908	arg1	pretreatments					919:931	pretreatments	919:931	pretreatments	919:931	RESULTS Probes' diffusion was mainly affected by probes size and pretreatments but only slightly by saccharification time.
30305844	4	43	theme	probes	755:760	arg1	diffusion					722:730	the diffusion	718:730	the diffusion of polyethylene glycol probes of different sizes	718:779	In addition to chemical composition and porosity analyses, the diffusion of polyethylene glycol probes of different sizes was measured at three different time points during the saccharification.
30305844	8	44	theme	initial	1592:1598	arg1	porosity					1600:1607	initial porosity	1592:1607	initial porosity	1592:1607	Testing the relationship with saccharification demonstrated that accessibility of 1.3-1.7-nm radius probes measured by FRAP on non-hydrolysed samples was highly correlated with poplar digestibility together with the measurement of initial porosity on the range 5-20 nm.
30305844	0	45	dep	probes	36:41	arg1	mobility					43:50	mobility	43:50	mobility	43:50	Dynamical assessment of fluorescent probes mobility in poplar cell walls reveals nanopores govern saccharification.
30305844	1	46	theme	lignocellulolytic	137:153	arg1	diffusion					164:172	lignocellulolytic enzymes' diffusion	137:172	lignocellulolytic enzymes' diffusion	137:172	BACKGROUND Improving lignocellulolytic enzymes' diffusion and accessibility to their substrate in the plant cell walls is recognised as a critical issue for optimising saccharification.
30305844	8	47	theme	non-hydrolysed	1488:1501	arg1	samples					1503:1509	non-hydrolysed samples	1488:1509	non-hydrolysed samples	1488:1509	Testing the relationship with saccharification demonstrated that accessibility of 1.3-1.7-nm radius probes measured by FRAP on non-hydrolysed samples was highly correlated with poplar digestibility together with the measurement of initial porosity on the range 5-20 nm.
30305844	4	48	theme	different	765:773	arg1	sizes					775:779	different sizes	765:779	different sizes	765:779	In addition to chemical composition and porosity analyses, the diffusion of polyethylene glycol probes of different sizes was measured at three different time points during the saccharification.
30305844	3	49	from	effects	594:600	arg1	composition					628:638	composition	628:638	composition	628:638	To address this issue, poplar fragments were submitted to hot water and ionic liquid pretreatments selected for their contrasted effects on both the structure and composition of lignocellulose.
30305844	3	49	from	effects	594:600	arg1	structure					614:622	structure	614:622	structure	614:622	To address this issue, poplar fragments were submitted to hot water and ionic liquid pretreatments selected for their contrasted effects on both the structure and composition of lignocellulose.
30305844	7	50	dep	times	1331:1335	arg1	size					1341:1344	the size	1337:1344	least five times the size of the probes	1320:1358	Porosity measurements showed that probes' diffusion was highly correlated with the amount of pores having a diameter at least five times the size of the probes.
30305844	4	51	theme	different	803:811	arg1	points					818:823	three different time points	797:823	three different time points during the saccharification	797:851	In addition to chemical composition and porosity analyses, the diffusion of polyethylene glycol probes of different sizes was measured at three different time points during the saccharification.
30305844	6	52	theme	relative	1163:1170	arg1	amount					1172:1177	amount	1172:1177	amount	1172:1177	This means that, despite the removal of polysaccharides during saccharification, diffusion of probes was not improved since they became hindered by changes in lignin conformation, whose relative amount increased over time.
30305844	8	53	theme	1.3-1.7-nm	1443:1452	arg1	radius probes					1454:1466	1.3-1.7-nm radius probes	1443:1466	1.3-1.7-nm radius probes measured by FRAP	1443:1483	Testing the relationship with saccharification demonstrated that accessibility of 1.3-1.7-nm radius probes measured by FRAP on non-hydrolysed samples was highly correlated with poplar digestibility together with the measurement of initial porosity on the range 5-20 nm.
30305844	1	54	theme	plant	218:222	arg1	walls					229:233	the plant cell walls	214:233	the plant cell walls	214:233	BACKGROUND Improving lignocellulolytic enzymes' diffusion and accessibility to their substrate in the plant cell walls is recognised as a critical issue for optimising saccharification.
30305844	3	55	theme	ionic	537:541	arg1	pretreatments					550:562	ionic liquid pretreatments	537:562	ionic liquid pretreatments	537:562	To address this issue, poplar fragments were submitted to hot water and ionic liquid pretreatments selected for their contrasted effects on both the structure and composition of lignocellulose.
30305844	6	56	theme	probes	1071:1076	arg1	diffusion					1058:1066	diffusion	1058:1066	diffusion of probes	1058:1076	This means that, despite the removal of polysaccharides during saccharification, diffusion of probes was not improved since they became hindered by changes in lignin conformation, whose relative amount increased over time.
30305844	0	57	theme	cell	62:65	arg1	walls					67:71	poplar cell walls	55:71	poplar cell walls	55:71	Dynamical assessment of fluorescent probes mobility in poplar cell walls reveals nanopores govern saccharification.
30305844	7	58	contain	having	1299:1304	arg1	pores					1293:1297	pores	1293:1297	pores having a diameter at least five times the size of the probes	1293:1358	Porosity measurements showed that probes' diffusion was highly correlated with the amount of pores having a diameter at least five times the size of the probes.
30305844	7	58	contain	having	1299:1304	arg2	diameter					1308:1315	a diameter	1306:1315	a diameter	1306:1315	Porosity measurements showed that probes' diffusion was highly correlated with the amount of pores having a diameter at least five times the size of the probes.
30305844	3	59	theme	lignocellulose	643:656	arg1	composition					628:638	composition	628:638	composition	628:638	To address this issue, poplar fragments were submitted to hot water and ionic liquid pretreatments selected for their contrasted effects on both the structure and composition of lignocellulose.
30305844	3	59	theme	lignocellulose	643:656	arg1	structure					614:622	structure	614:622	structure	614:622	To address this issue, poplar fragments were submitted to hot water and ionic liquid pretreatments selected for their contrasted effects on both the structure and composition of lignocellulose.
30305844	3	60	theme	liquid	543:548	arg1	pretreatments					550:562	ionic liquid pretreatments	537:562	ionic liquid pretreatments	537:562	To address this issue, poplar fragments were submitted to hot water and ionic liquid pretreatments selected for their contrasted effects on both the structure and composition of lignocellulose.
30305844	7	61	theme	pores	1293:1297	arg1	pores					1293:1297	pores	1293:1297	pores having a diameter at least five times the size of the probes	1293:1358	Porosity measurements showed that probes' diffusion was highly correlated with the amount of pores having a diameter at least five times the size of the probes.
30305844	7	61	theme	pores	1293:1297	arg1	amount					1283:1288	the amount	1279:1288	the amount of pores having a diameter at least five times the size of the probes	1279:1358	Porosity measurements showed that probes' diffusion was highly correlated with the amount of pores having a diameter at least five times the size of the probes.
30305844	1	62	theme	critical	254:261	arg1	issue					263:267	a critical issue	252:267	a critical issue for optimising saccharification	252:299	BACKGROUND Improving lignocellulolytic enzymes' diffusion and accessibility to their substrate in the plant cell walls is recognised as a critical issue for optimising saccharification.
30305844	1	62	theme	critical	254:261	arg1	BACKGROUND					116:125	BACKGROUND	116:125	BACKGROUND Improving lignocellulolytic enzymes' diffusion and accessibility to their substrate in the plant cell walls	116:233	BACKGROUND Improving lignocellulolytic enzymes' diffusion and accessibility to their substrate in the plant cell walls is recognised as a critical issue for optimising saccharification.
29763441	8	0	from	sources	1259:1265	arg1	view					1185:1188	view	1185:1188	view of an integrated valorization	1185:1218	In view of an integrated valorization, Quercus faginea barks are interesting sources of polar compounds including phenols and polyphenols with possible interesting bioactivities, while the sterols and triterpenes contained in the lipophilic extracts are also valuable bioactive compounds or chemical intermediates for specific high-value market niches, such as cosmetics, pharmaceuticals and biomedicine.
29763441	8	1	theme	lipophilic	1412:1421	arg1	extracts					1423:1430	the lipophilic extracts	1408:1430	the lipophilic extracts	1408:1430	In view of an integrated valorization, Quercus faginea barks are interesting sources of polar compounds including phenols and polyphenols with possible interesting bioactivities, while the sterols and triterpenes contained in the lipophilic extracts are also valuable bioactive compounds or chemical intermediates for specific high-value market niches, such as cosmetics, pharmaceuticals and biomedicine.
29763441	4	2	theme	ω-hydroxyacids	509:522	arg1	%					528:528	ω-hydroxyacids 46.3%	509:528	ω-hydroxyacids 46.3% of total compounds	509:547	The suberin composition was: ω-hydroxyacids 46.3% of total compounds, ɑ,ω-alkanoic diacids 22.3%, alkanoic acids 5.9%, alkanols 6.7% and aromatics 6.9% (ferulic acid 4.0%).
29763441	4	2	theme	ω-hydroxyacids	509:522	arg1	compounds					539:547	total compounds	533:547	total compounds	533:547	The suberin composition was: ω-hydroxyacids 46.3% of total compounds, ɑ,ω-alkanoic diacids 22.3%, alkanoic acids 5.9%, alkanols 6.7% and aromatics 6.9% (ferulic acid 4.0%).
29763441	4	2	theme	ω-hydroxyacids	509:522	arg1	composition					492:502	The suberin composition	480:502	The suberin composition	480:502	The suberin composition was: ω-hydroxyacids 46.3% of total compounds, ɑ,ω-alkanoic diacids 22.3%, alkanoic acids 5.9%, alkanols 6.7% and aromatics 6.9% (ferulic acid 4.0%).
29763441	3	3	theme	uronic	466:471	arg1	acids					473:477	uronic acids	466:477	uronic acids	466:477	The polysaccharides were composed mainly of glucose and xylose (50.3% and 35.1% of all monosaccharides respectively) with 4.8% of uronic acids.
29763441	4	4	theme	diacids	563:569	arg1	compounds					539:547	total compounds	533:547	total compounds	533:547	The suberin composition was: ω-hydroxyacids 46.3% of total compounds, ɑ,ω-alkanoic diacids 22.3%, alkanoic acids 5.9%, alkanols 6.7% and aromatics 6.9% (ferulic acid 4.0%).
29763441	4	4	theme	diacids	563:569	arg1	%					575:575	ω-alkanoic diacids 22.3%	552:575	ω-alkanoic diacids 22.3%	552:575	The suberin composition was: ω-hydroxyacids 46.3% of total compounds, ɑ,ω-alkanoic diacids 22.3%, alkanoic acids 5.9%, alkanols 6.7% and aromatics 6.9% (ferulic acid 4.0%).
29763441	2	5	theme	composition	256:266	arg1	%					277:277	the following composition: ash 14.6%	242:277	the following composition: ash 14.6%	242:277	The barks showed the following composition: ash 14.6%, total extractives 13.2%, suberin 2.9% and lignin 28.2%.
29763441	8	6	theme	valuable	1441:1448	arg1	compounds					1460:1468	valuable bioactive compounds	1441:1468	valuable bioactive compounds	1441:1468	In view of an integrated valorization, Quercus faginea barks are interesting sources of polar compounds including phenols and polyphenols with possible interesting bioactivities, while the sterols and triterpenes contained in the lipophilic extracts are also valuable bioactive compounds or chemical intermediates for specific high-value market niches, such as cosmetics, pharmaceuticals and biomedicine.
29763441	8	6	theme	valuable	1441:1448	arg1	pharmaceuticals					1554:1568	pharmaceuticals	1554:1568	pharmaceuticals	1554:1568	In view of an integrated valorization, Quercus faginea barks are interesting sources of polar compounds including phenols and polyphenols with possible interesting bioactivities, while the sterols and triterpenes contained in the lipophilic extracts are also valuable bioactive compounds or chemical intermediates for specific high-value market niches, such as cosmetics, pharmaceuticals and biomedicine.
29763441	8	6	theme	valuable	1441:1448	arg1	sterols					1371:1377	sterols	1371:1377	sterols	1371:1377	In view of an integrated valorization, Quercus faginea barks are interesting sources of polar compounds including phenols and polyphenols with possible interesting bioactivities, while the sterols and triterpenes contained in the lipophilic extracts are also valuable bioactive compounds or chemical intermediates for specific high-value market niches, such as cosmetics, pharmaceuticals and biomedicine.
29763441	8	6	theme	valuable	1441:1448	arg1	triterpenes					1383:1393	triterpenes	1383:1393	triterpenes	1383:1393	In view of an integrated valorization, Quercus faginea barks are interesting sources of polar compounds including phenols and polyphenols with possible interesting bioactivities, while the sterols and triterpenes contained in the lipophilic extracts are also valuable bioactive compounds or chemical intermediates for specific high-value market niches, such as cosmetics, pharmaceuticals and biomedicine.
29763441	8	6	theme	valuable	1441:1448	arg1	biomedicine					1574:1584	biomedicine	1574:1584	biomedicine	1574:1584	In view of an integrated valorization, Quercus faginea barks are interesting sources of polar compounds including phenols and polyphenols with possible interesting bioactivities, while the sterols and triterpenes contained in the lipophilic extracts are also valuable bioactive compounds or chemical intermediates for specific high-value market niches, such as cosmetics, pharmaceuticals and biomedicine.
29763441	8	6	theme	valuable	1441:1448	arg1	cosmetics					1543:1551	cosmetics	1543:1551	cosmetics	1543:1551	In view of an integrated valorization, Quercus faginea barks are interesting sources of polar compounds including phenols and polyphenols with possible interesting bioactivities, while the sterols and triterpenes contained in the lipophilic extracts are also valuable bioactive compounds or chemical intermediates for specific high-value market niches, such as cosmetics, pharmaceuticals and biomedicine.
29763441	6	7	theme	Trolox	943:948	arg1	equivalents/g					950:962	1567 mg Trolox equivalents/g	935:962	1567 mg Trolox equivalents/g of extract	935:973	The antioxidant activity was very high corresponding to 1567 mg Trolox equivalents/g of extract, and an IC50 of 2.63 μg extract/ml.
29763441	2	8	theme	total	280:284	arg1	%					302:302	total extractives 13.2%	280:302	total extractives 13.2%	280:302	The barks showed the following composition: ash 14.6%, total extractives 13.2%, suberin 2.9% and lignin 28.2%.
29763441	6	9	theme	1567	935:938	arg1	mg					940:941	mg	940:941	mg	940:941	The antioxidant activity was very high corresponding to 1567 mg Trolox equivalents/g of extract, and an IC50 of 2.63 μg extract/ml.
29763441	5	10	theme	mg	859:860	arg1	CE/g					862:865	207.7 mg CE/g	853:865	207.7 mg CE/g	853:865	Polar extracts (ethanol-water) had a high phenolic content of 630.3 mg of gallic acid equivalents (GAE)/g of extract, condensed tannins 220.7 mg of catechin equivalents (CE)/g extract, and flavonoids 207.7 mg CE/g of extract.
29763441	8	11	theme	chemical	1473:1480	arg1	biomedicine					1574:1584	biomedicine	1574:1584	biomedicine	1574:1584	In view of an integrated valorization, Quercus faginea barks are interesting sources of polar compounds including phenols and polyphenols with possible interesting bioactivities, while the sterols and triterpenes contained in the lipophilic extracts are also valuable bioactive compounds or chemical intermediates for specific high-value market niches, such as cosmetics, pharmaceuticals and biomedicine.
29763441	8	11	theme	chemical	1473:1480	arg1	intermediates					1482:1494	chemical intermediates	1473:1494	chemical intermediates	1473:1494	In view of an integrated valorization, Quercus faginea barks are interesting sources of polar compounds including phenols and polyphenols with possible interesting bioactivities, while the sterols and triterpenes contained in the lipophilic extracts are also valuable bioactive compounds or chemical intermediates for specific high-value market niches, such as cosmetics, pharmaceuticals and biomedicine.
29763441	8	11	theme	chemical	1473:1480	arg1	pharmaceuticals					1554:1568	pharmaceuticals	1554:1568	pharmaceuticals	1554:1568	In view of an integrated valorization, Quercus faginea barks are interesting sources of polar compounds including phenols and polyphenols with possible interesting bioactivities, while the sterols and triterpenes contained in the lipophilic extracts are also valuable bioactive compounds or chemical intermediates for specific high-value market niches, such as cosmetics, pharmaceuticals and biomedicine.
29763441	8	11	theme	chemical	1473:1480	arg1	cosmetics					1543:1551	cosmetics	1543:1551	cosmetics	1543:1551	In view of an integrated valorization, Quercus faginea barks are interesting sources of polar compounds including phenols and polyphenols with possible interesting bioactivities, while the sterols and triterpenes contained in the lipophilic extracts are also valuable bioactive compounds or chemical intermediates for specific high-value market niches, such as cosmetics, pharmaceuticals and biomedicine.
29763441	5	12	theme	high	690:693	arg1	content					704:710	a high phenolic content	688:710	a high phenolic content of 630.3 mg of gallic acid equivalents (GAE)/g of extract, condensed tannins 220.7 mg of catechin equivalents (CE)/g extract, and flavonoids 207.7 mg CE/g of extract	688:876	Polar extracts (ethanol-water) had a high phenolic content of 630.3 mg of gallic acid equivalents (GAE)/g of extract, condensed tannins 220.7 mg of catechin equivalents (CE)/g extract, and flavonoids 207.7 mg CE/g of extract.
29763441	6	13	theme	μg	996:997	arg1	extract/ml					999:1008	2.63 μg extract/ml	991:1008	2.63 μg extract/ml	991:1008	The antioxidant activity was very high corresponding to 1567 mg Trolox equivalents/g of extract, and an IC50 of 2.63 μg extract/ml.
29763441	8	14	theme	high-value	1509:1518	arg1	niches					1527:1532	specific high-value market niches	1500:1532	specific high-value market niches	1500:1532	In view of an integrated valorization, Quercus faginea barks are interesting sources of polar compounds including phenols and polyphenols with possible interesting bioactivities, while the sterols and triterpenes contained in the lipophilic extracts are also valuable bioactive compounds or chemical intermediates for specific high-value market niches, such as cosmetics, pharmaceuticals and biomedicine.
29763441	8	15	with	phenols	1296:1302	arg1	bioactivities					1346:1358	possible interesting bioactivities	1325:1358	possible interesting bioactivities	1325:1358	In view of an integrated valorization, Quercus faginea barks are interesting sources of polar compounds including phenols and polyphenols with possible interesting bioactivities, while the sterols and triterpenes contained in the lipophilic extracts are also valuable bioactive compounds or chemical intermediates for specific high-value market niches, such as cosmetics, pharmaceuticals and biomedicine.
29763441	5	16	theme	flavonoids	842:851	arg1	content					704:710	a high phenolic content	688:710	a high phenolic content of 630.3 mg of gallic acid equivalents (GAE)/g of extract, condensed tannins 220.7 mg of catechin equivalents (CE)/g extract, and flavonoids 207.7 mg CE/g of extract	688:876	Polar extracts (ethanol-water) had a high phenolic content of 630.3 mg of gallic acid equivalents (GAE)/g of extract, condensed tannins 220.7 mg of catechin equivalents (CE)/g extract, and flavonoids 207.7 mg CE/g of extract.
29763441	1	17	from	sites	170:174	arg1	trees					155:159	Quercus faginea mature trees	132:159	Quercus faginea mature trees from two sites	132:174	The bark from Quercus faginea mature trees from two sites was chemically characterized for the first time.
29763441	7	18	theme	compounds	1105:1113	arg1	%					1096:1096	12.3%	1092:1096	12.3% of all compounds	1092:1113	The lipophilic extracts were constituted mainly by glycerol and its derivatives (12.3% of all compounds), alkanoic acids (27.8%), sterols (11.5%) and triterpenes (17.8%).
29763441	7	18	theme	compounds	1105:1113	arg1	compounds					1105:1113	all compounds	1101:1113	all compounds	1101:1113	The lipophilic extracts were constituted mainly by glycerol and its derivatives (12.3% of all compounds), alkanoic acids (27.8%), sterols (11.5%) and triterpenes (17.8%).
29763441	4	19	theme	alkanoic	578:585	arg1	compounds					539:547	total compounds	533:547	total compounds	533:547	The suberin composition was: ω-hydroxyacids 46.3% of total compounds, ɑ,ω-alkanoic diacids 22.3%, alkanoic acids 5.9%, alkanols 6.7% and aromatics 6.9% (ferulic acid 4.0%).
29763441	4	19	theme	alkanoic	578:585	arg1	%					596:596	alkanoic acids 5.9%	578:596	alkanoic acids 5.9%	578:596	The suberin composition was: ω-hydroxyacids 46.3% of total compounds, ɑ,ω-alkanoic diacids 22.3%, alkanoic acids 5.9%, alkanols 6.7% and aromatics 6.9% (ferulic acid 4.0%).
29763441	1	20	theme	faginea	140:146	arg1	trees					155:159	Quercus faginea mature trees	132:159	Quercus faginea mature trees from two sites	132:174	The bark from Quercus faginea mature trees from two sites was chemically characterized for the first time.
29763441	5	21	theme	acid	734:737	arg1	/g					756:757	gallic acid equivalents (GAE)/g	727:757	gallic acid equivalents (GAE)/g of extract	727:768	Polar extracts (ethanol-water) had a high phenolic content of 630.3 mg of gallic acid equivalents (GAE)/g of extract, condensed tannins 220.7 mg of catechin equivalents (CE)/g extract, and flavonoids 207.7 mg CE/g of extract.
29763441	8	22	theme	integrated	1196:1205	arg1	valorization					1207:1218	an integrated valorization	1193:1218	an integrated valorization	1193:1218	In view of an integrated valorization, Quercus faginea barks are interesting sources of polar compounds including phenols and polyphenols with possible interesting bioactivities, while the sterols and triterpenes contained in the lipophilic extracts are also valuable bioactive compounds or chemical intermediates for specific high-value market niches, such as cosmetics, pharmaceuticals and biomedicine.
29763441	0	23	theme	lipophilic	87:96	arg1	extracts					108:115	their lipophilic and polar extracts	81:115	their lipophilic and polar extracts	81:115	Chemical composition of barks from Quercus faginea trees and characterization of their lipophilic and polar extracts.
29763441	2	24	theme	ash	269:271	arg1	%					277:277	the following composition: ash 14.6%	242:277	the following composition: ash 14.6%	242:277	The barks showed the following composition: ash 14.6%, total extractives 13.2%, suberin 2.9% and lignin 28.2%.
29763441	5	25	theme	tannins	781:787	arg1	mg					795:796	condensed tannins 220.7 mg	771:796	condensed tannins 220.7 mg of catechin equivalents (CE)/g extract	771:835	Polar extracts (ethanol-water) had a high phenolic content of 630.3 mg of gallic acid equivalents (GAE)/g of extract, condensed tannins 220.7 mg of catechin equivalents (CE)/g extract, and flavonoids 207.7 mg CE/g of extract.
29763441	0	26	from	faginea	43:49	arg1	barks					24:28	barks	24:28	barks from Quercus faginea	24:49	Chemical composition of barks from Quercus faginea trees and characterization of their lipophilic and polar extracts.
29763441	0	26	from	faginea	43:49	arg1	composition					9:19	Chemical composition	0:19	Chemical composition of barks from Quercus faginea	0:49	Chemical composition of barks from Quercus faginea trees and characterization of their lipophilic and polar extracts.
29763441	0	27	theme	polar	102:106	arg1	extracts					108:115	their lipophilic and polar extracts	81:115	their lipophilic and polar extracts	81:115	Chemical composition of barks from Quercus faginea trees and characterization of their lipophilic and polar extracts.
29763441	5	28	theme	mg	795:796	arg1	content					704:710	a high phenolic content	688:710	a high phenolic content of 630.3 mg of gallic acid equivalents (GAE)/g of extract, condensed tannins 220.7 mg of catechin equivalents (CE)/g extract, and flavonoids 207.7 mg CE/g of extract	688:876	Polar extracts (ethanol-water) had a high phenolic content of 630.3 mg of gallic acid equivalents (GAE)/g of extract, condensed tannins 220.7 mg of catechin equivalents (CE)/g extract, and flavonoids 207.7 mg CE/g of extract.
29763441	8	29	theme	faginea	1229:1235	arg1	sources					1259:1265	interesting sources	1247:1265	interesting sources of polar compounds including phenols and polyphenols with possible interesting bioactivities	1247:1358	In view of an integrated valorization, Quercus faginea barks are interesting sources of polar compounds including phenols and polyphenols with possible interesting bioactivities, while the sterols and triterpenes contained in the lipophilic extracts are also valuable bioactive compounds or chemical intermediates for specific high-value market niches, such as cosmetics, pharmaceuticals and biomedicine.
29763441	8	29	theme	faginea	1229:1235	arg1	barks					1237:1241	Quercus faginea barks	1221:1241	Quercus faginea barks	1221:1241	In view of an integrated valorization, Quercus faginea barks are interesting sources of polar compounds including phenols and polyphenols with possible interesting bioactivities, while the sterols and triterpenes contained in the lipophilic extracts are also valuable bioactive compounds or chemical intermediates for specific high-value market niches, such as cosmetics, pharmaceuticals and biomedicine.
29763441	3	30	dep	composed	361:368	arg1	%					404:404	50.3%	400:404	50.3%	400:404	The polysaccharides were composed mainly of glucose and xylose (50.3% and 35.1% of all monosaccharides respectively) with 4.8% of uronic acids.
29763441	3	30	dep	composed	361:368	arg1	%					414:414	35.1%	410:414	35.1% of all monosaccharides	410:437	The polysaccharides were composed mainly of glucose and xylose (50.3% and 35.1% of all monosaccharides respectively) with 4.8% of uronic acids.
29763441	3	30	dep	composed	361:368	arg1	monosaccharides					423:437	all monosaccharides	419:437	all monosaccharides	419:437	The polysaccharides were composed mainly of glucose and xylose (50.3% and 35.1% of all monosaccharides respectively) with 4.8% of uronic acids.
29763441	0	31	theme	Chemical	0:7	arg1	composition					9:19	Chemical composition	0:19	Chemical composition of barks from Quercus faginea	0:49	Chemical composition of barks from Quercus faginea trees and characterization of their lipophilic and polar extracts.
29763441	5	32	dep	mg	721:722	arg1	CE/g					862:865	207.7 mg CE/g	853:865	207.7 mg CE/g	853:865	Polar extracts (ethanol-water) had a high phenolic content of 630.3 mg of gallic acid equivalents (GAE)/g of extract, condensed tannins 220.7 mg of catechin equivalents (CE)/g extract, and flavonoids 207.7 mg CE/g of extract.
29763441	1	33	theme	Quercus	132:138	arg1	trees					155:159	Quercus faginea mature trees	132:159	Quercus faginea mature trees from two sites	132:174	The bark from Quercus faginea mature trees from two sites was chemically characterized for the first time.
29763441	8	34	theme	polar	1270:1274	arg1	compounds					1276:1284	polar compounds	1270:1284	polar compounds including phenols and polyphenols with possible interesting bioactivities	1270:1358	In view of an integrated valorization, Quercus faginea barks are interesting sources of polar compounds including phenols and polyphenols with possible interesting bioactivities, while the sterols and triterpenes contained in the lipophilic extracts are also valuable bioactive compounds or chemical intermediates for specific high-value market niches, such as cosmetics, pharmaceuticals and biomedicine.
29763441	8	34	theme	polar	1270:1274	arg1	polyphenols					1308:1318	polyphenols	1308:1318	polyphenols	1308:1318	In view of an integrated valorization, Quercus faginea barks are interesting sources of polar compounds including phenols and polyphenols with possible interesting bioactivities, while the sterols and triterpenes contained in the lipophilic extracts are also valuable bioactive compounds or chemical intermediates for specific high-value market niches, such as cosmetics, pharmaceuticals and biomedicine.
29763441	8	34	theme	polar	1270:1274	arg1	phenols					1296:1302	phenols	1296:1302	phenols	1296:1302	In view of an integrated valorization, Quercus faginea barks are interesting sources of polar compounds including phenols and polyphenols with possible interesting bioactivities, while the sterols and triterpenes contained in the lipophilic extracts are also valuable bioactive compounds or chemical intermediates for specific high-value market niches, such as cosmetics, pharmaceuticals and biomedicine.
29763441	2	35	theme	suberin	305:311	arg1	%					316:316	suberin 2.9%	305:316	suberin 2.9%	305:316	The barks showed the following composition: ash 14.6%, total extractives 13.2%, suberin 2.9% and lignin 28.2%.
29763441	4	36	theme	%	649:649	arg1	acid					641:644	ferulic acid	633:644	ferulic acid 4.0%	633:649	The suberin composition was: ω-hydroxyacids 46.3% of total compounds, ɑ,ω-alkanoic diacids 22.3%, alkanoic acids 5.9%, alkanols 6.7% and aromatics 6.9% (ferulic acid 4.0%).
29763441	1	37	theme	first	213:217	arg1	time					219:222	the first time	209:222	the first time	209:222	The bark from Quercus faginea mature trees from two sites was chemically characterized for the first time.
29763441	5	38	theme	catechin	801:808	arg1	equivalents					810:820	catechin equivalents	801:820	catechin equivalents (CE)/g extract	801:835	Polar extracts (ethanol-water) had a high phenolic content of 630.3 mg of gallic acid equivalents (GAE)/g of extract, condensed tannins 220.7 mg of catechin equivalents (CE)/g extract, and flavonoids 207.7 mg CE/g of extract.
29763441	5	38	theme	catechin	801:808	arg1	CE					823:824	CE	823:824	CE	823:824	Polar extracts (ethanol-water) had a high phenolic content of 630.3 mg of gallic acid equivalents (GAE)/g of extract, condensed tannins 220.7 mg of catechin equivalents (CE)/g extract, and flavonoids 207.7 mg CE/g of extract.
29763441	2	39	theme	lignin	322:327	arg1	%					333:333	lignin 28.2%	322:333	lignin 28.2%	322:333	The barks showed the following composition: ash 14.6%, total extractives 13.2%, suberin 2.9% and lignin 28.2%.
29763441	6	40	theme	antioxidant	883:893	arg1	activity					895:902	The antioxidant activity	879:902	The antioxidant activity	879:902	The antioxidant activity was very high corresponding to 1567 mg Trolox equivalents/g of extract, and an IC50 of 2.63 μg extract/ml.
29763441	6	40	theme	antioxidant	883:893	arg1	high					913:916	high	913:916	high	913:916	The antioxidant activity was very high corresponding to 1567 mg Trolox equivalents/g of extract, and an IC50 of 2.63 μg extract/ml.
29763441	3	41	theme	monosaccharides	423:437	arg1	%					404:404	50.3%	400:404	50.3%	400:404	The polysaccharides were composed mainly of glucose and xylose (50.3% and 35.1% of all monosaccharides respectively) with 4.8% of uronic acids.
29763441	3	41	theme	monosaccharides	423:437	arg1	%					414:414	35.1%	410:414	35.1% of all monosaccharides	410:437	The polysaccharides were composed mainly of glucose and xylose (50.3% and 35.1% of all monosaccharides respectively) with 4.8% of uronic acids.
29763441	3	41	theme	monosaccharides	423:437	arg1	monosaccharides					423:437	all monosaccharides	419:437	all monosaccharides	419:437	The polysaccharides were composed mainly of glucose and xylose (50.3% and 35.1% of all monosaccharides respectively) with 4.8% of uronic acids.
29763441	7	42	theme	lipophilic	1015:1024	arg1	extracts					1026:1033	The lipophilic extracts	1011:1033	The lipophilic extracts	1011:1033	The lipophilic extracts were constituted mainly by glycerol and its derivatives (12.3% of all compounds), alkanoic acids (27.8%), sterols (11.5%) and triterpenes (17.8%).
29763441	8	43	theme	interesting	1334:1344	arg1	bioactivities					1346:1358	possible interesting bioactivities	1325:1358	possible interesting bioactivities	1325:1358	In view of an integrated valorization, Quercus faginea barks are interesting sources of polar compounds including phenols and polyphenols with possible interesting bioactivities, while the sterols and triterpenes contained in the lipophilic extracts are also valuable bioactive compounds or chemical intermediates for specific high-value market niches, such as cosmetics, pharmaceuticals and biomedicine.
29763441	5	44	theme	gallic	727:732	arg1	acid					734:737	gallic acid	727:737	gallic acid equivalents (GAE)/g of extract	727:768	Polar extracts (ethanol-water) had a high phenolic content of 630.3 mg of gallic acid equivalents (GAE)/g of extract, condensed tannins 220.7 mg of catechin equivalents (CE)/g extract, and flavonoids 207.7 mg CE/g of extract.
29763441	8	45	with	polyphenols	1308:1318	arg1	bioactivities					1346:1358	possible interesting bioactivities	1325:1358	possible interesting bioactivities	1325:1358	In view of an integrated valorization, Quercus faginea barks are interesting sources of polar compounds including phenols and polyphenols with possible interesting bioactivities, while the sterols and triterpenes contained in the lipophilic extracts are also valuable bioactive compounds or chemical intermediates for specific high-value market niches, such as cosmetics, pharmaceuticals and biomedicine.
29763441	5	46	theme	phenolic	695:702	arg1	content					704:710	a high phenolic content	688:710	a high phenolic content of 630.3 mg of gallic acid equivalents (GAE)/g of extract, condensed tannins 220.7 mg of catechin equivalents (CE)/g extract, and flavonoids 207.7 mg CE/g of extract	688:876	Polar extracts (ethanol-water) had a high phenolic content of 630.3 mg of gallic acid equivalents (GAE)/g of extract, condensed tannins 220.7 mg of catechin equivalents (CE)/g extract, and flavonoids 207.7 mg CE/g of extract.
29763441	4	47	theme	ω-alkanoic	552:561	arg1	compounds					539:547	total compounds	533:547	total compounds	533:547	The suberin composition was: ω-hydroxyacids 46.3% of total compounds, ɑ,ω-alkanoic diacids 22.3%, alkanoic acids 5.9%, alkanols 6.7% and aromatics 6.9% (ferulic acid 4.0%).
29763441	4	47	theme	ω-alkanoic	552:561	arg1	%					575:575	ω-alkanoic diacids 22.3%	552:575	ω-alkanoic diacids 22.3%	552:575	The suberin composition was: ω-hydroxyacids 46.3% of total compounds, ɑ,ω-alkanoic diacids 22.3%, alkanoic acids 5.9%, alkanols 6.7% and aromatics 6.9% (ferulic acid 4.0%).
29763441	2	48	theme	extractives	286:296	arg1	%					302:302	total extractives 13.2%	280:302	total extractives 13.2%	280:302	The barks showed the following composition: ash 14.6%, total extractives 13.2%, suberin 2.9% and lignin 28.2%.
29763441	8	49	theme	valorization	1207:1218	arg1	view					1185:1188	view	1185:1188	view of an integrated valorization	1185:1218	In view of an integrated valorization, Quercus faginea barks are interesting sources of polar compounds including phenols and polyphenols with possible interesting bioactivities, while the sterols and triterpenes contained in the lipophilic extracts are also valuable bioactive compounds or chemical intermediates for specific high-value market niches, such as cosmetics, pharmaceuticals and biomedicine.
29763441	6	50	theme	mg	940:941	arg1	equivalents/g					950:962	1567 mg Trolox equivalents/g	935:962	1567 mg Trolox equivalents/g of extract	935:973	The antioxidant activity was very high corresponding to 1567 mg Trolox equivalents/g of extract, and an IC50 of 2.63 μg extract/ml.
29763441	3	51	theme	acids	473:477	arg1	%					461:461	4.8%	458:461	4.8% of uronic acids	458:477	The polysaccharides were composed mainly of glucose and xylose (50.3% and 35.1% of all monosaccharides respectively) with 4.8% of uronic acids.
29763441	3	51	theme	acids	473:477	arg1	acids					473:477	uronic acids	466:477	uronic acids	466:477	The polysaccharides were composed mainly of glucose and xylose (50.3% and 35.1% of all monosaccharides respectively) with 4.8% of uronic acids.
29763441	8	52	theme	bioactive	1450:1458	arg1	compounds					1460:1468	valuable bioactive compounds	1441:1468	valuable bioactive compounds	1441:1468	In view of an integrated valorization, Quercus faginea barks are interesting sources of polar compounds including phenols and polyphenols with possible interesting bioactivities, while the sterols and triterpenes contained in the lipophilic extracts are also valuable bioactive compounds or chemical intermediates for specific high-value market niches, such as cosmetics, pharmaceuticals and biomedicine.
29763441	8	52	theme	bioactive	1450:1458	arg1	pharmaceuticals					1554:1568	pharmaceuticals	1554:1568	pharmaceuticals	1554:1568	In view of an integrated valorization, Quercus faginea barks are interesting sources of polar compounds including phenols and polyphenols with possible interesting bioactivities, while the sterols and triterpenes contained in the lipophilic extracts are also valuable bioactive compounds or chemical intermediates for specific high-value market niches, such as cosmetics, pharmaceuticals and biomedicine.
29763441	8	52	theme	bioactive	1450:1458	arg1	sterols					1371:1377	sterols	1371:1377	sterols	1371:1377	In view of an integrated valorization, Quercus faginea barks are interesting sources of polar compounds including phenols and polyphenols with possible interesting bioactivities, while the sterols and triterpenes contained in the lipophilic extracts are also valuable bioactive compounds or chemical intermediates for specific high-value market niches, such as cosmetics, pharmaceuticals and biomedicine.
29763441	8	52	theme	bioactive	1450:1458	arg1	triterpenes					1383:1393	triterpenes	1383:1393	triterpenes	1383:1393	In view of an integrated valorization, Quercus faginea barks are interesting sources of polar compounds including phenols and polyphenols with possible interesting bioactivities, while the sterols and triterpenes contained in the lipophilic extracts are also valuable bioactive compounds or chemical intermediates for specific high-value market niches, such as cosmetics, pharmaceuticals and biomedicine.
29763441	8	52	theme	bioactive	1450:1458	arg1	biomedicine					1574:1584	biomedicine	1574:1584	biomedicine	1574:1584	In view of an integrated valorization, Quercus faginea barks are interesting sources of polar compounds including phenols and polyphenols with possible interesting bioactivities, while the sterols and triterpenes contained in the lipophilic extracts are also valuable bioactive compounds or chemical intermediates for specific high-value market niches, such as cosmetics, pharmaceuticals and biomedicine.
29763441	8	52	theme	bioactive	1450:1458	arg1	cosmetics					1543:1551	cosmetics	1543:1551	cosmetics	1543:1551	In view of an integrated valorization, Quercus faginea barks are interesting sources of polar compounds including phenols and polyphenols with possible interesting bioactivities, while the sterols and triterpenes contained in the lipophilic extracts are also valuable bioactive compounds or chemical intermediates for specific high-value market niches, such as cosmetics, pharmaceuticals and biomedicine.
29763441	4	53	dep	%	630:630	arg1	acid					641:644	ferulic acid	633:644	ferulic acid 4.0%	633:649	The suberin composition was: ω-hydroxyacids 46.3% of total compounds, ɑ,ω-alkanoic diacids 22.3%, alkanoic acids 5.9%, alkanols 6.7% and aromatics 6.9% (ferulic acid 4.0%).
29763441	4	54	theme	compounds	539:547	arg1	compounds					539:547	total compounds	533:547	total compounds	533:547	The suberin composition was: ω-hydroxyacids 46.3% of total compounds, ɑ,ω-alkanoic diacids 22.3%, alkanoic acids 5.9%, alkanols 6.7% and aromatics 6.9% (ferulic acid 4.0%).
29763441	4	54	theme	compounds	539:547	arg1	%					630:630	aromatics 6.9%	617:630	aromatics 6.9% (ferulic acid 4.0%)	617:650	The suberin composition was: ω-hydroxyacids 46.3% of total compounds, ɑ,ω-alkanoic diacids 22.3%, alkanoic acids 5.9%, alkanols 6.7% and aromatics 6.9% (ferulic acid 4.0%).
29763441	4	54	theme	compounds	539:547	arg1	ɑ					550:550	ɑ	550:550	ɑ	550:550	The suberin composition was: ω-hydroxyacids 46.3% of total compounds, ɑ,ω-alkanoic diacids 22.3%, alkanoic acids 5.9%, alkanols 6.7% and aromatics 6.9% (ferulic acid 4.0%).
29763441	4	54	theme	compounds	539:547	arg1	%					528:528	ω-hydroxyacids 46.3%	509:528	ω-hydroxyacids 46.3% of total compounds	509:547	The suberin composition was: ω-hydroxyacids 46.3% of total compounds, ɑ,ω-alkanoic diacids 22.3%, alkanoic acids 5.9%, alkanols 6.7% and aromatics 6.9% (ferulic acid 4.0%).
29763441	4	54	theme	compounds	539:547	arg1	%					611:611	alkanols 6.7%	599:611	alkanols 6.7%	599:611	The suberin composition was: ω-hydroxyacids 46.3% of total compounds, ɑ,ω-alkanoic diacids 22.3%, alkanoic acids 5.9%, alkanols 6.7% and aromatics 6.9% (ferulic acid 4.0%).
29763441	4	54	theme	compounds	539:547	arg1	composition					492:502	The suberin composition	480:502	The suberin composition	480:502	The suberin composition was: ω-hydroxyacids 46.3% of total compounds, ɑ,ω-alkanoic diacids 22.3%, alkanoic acids 5.9%, alkanols 6.7% and aromatics 6.9% (ferulic acid 4.0%).
29763441	4	54	theme	compounds	539:547	arg1	%					596:596	alkanoic acids 5.9%	578:596	alkanoic acids 5.9%	578:596	The suberin composition was: ω-hydroxyacids 46.3% of total compounds, ɑ,ω-alkanoic diacids 22.3%, alkanoic acids 5.9%, alkanols 6.7% and aromatics 6.9% (ferulic acid 4.0%).
29763441	4	54	theme	compounds	539:547	arg1	%					575:575	ω-alkanoic diacids 22.3%	552:575	ω-alkanoic diacids 22.3%	552:575	The suberin composition was: ω-hydroxyacids 46.3% of total compounds, ɑ,ω-alkanoic diacids 22.3%, alkanoic acids 5.9%, alkanols 6.7% and aromatics 6.9% (ferulic acid 4.0%).
29763441	6	55	theme	extract/ml	999:1008	arg1	IC50					983:986	an IC50	980:986	an IC50 of 2.63 μg extract/ml	980:1008	The antioxidant activity was very high corresponding to 1567 mg Trolox equivalents/g of extract, and an IC50 of 2.63 μg extract/ml.
29763441	6	55	theme	extract/ml	999:1008	arg1	equivalents/g					950:962	1567 mg Trolox equivalents/g	935:962	1567 mg Trolox equivalents/g of extract	935:973	The antioxidant activity was very high corresponding to 1567 mg Trolox equivalents/g of extract, and an IC50 of 2.63 μg extract/ml.
29763441	5	56	theme	extract	870:876	arg1	content					704:710	a high phenolic content	688:710	a high phenolic content of 630.3 mg of gallic acid equivalents (GAE)/g of extract, condensed tannins 220.7 mg of catechin equivalents (CE)/g extract, and flavonoids 207.7 mg CE/g of extract	688:876	Polar extracts (ethanol-water) had a high phenolic content of 630.3 mg of gallic acid equivalents (GAE)/g of extract, condensed tannins 220.7 mg of catechin equivalents (CE)/g extract, and flavonoids 207.7 mg CE/g of extract.
29763441	5	57	theme	equivalents	739:749	arg1	/g					756:757	gallic acid equivalents (GAE)/g	727:757	gallic acid equivalents (GAE)/g of extract	727:768	Polar extracts (ethanol-water) had a high phenolic content of 630.3 mg of gallic acid equivalents (GAE)/g of extract, condensed tannins 220.7 mg of catechin equivalents (CE)/g extract, and flavonoids 207.7 mg CE/g of extract.
29763441	6	58	theme	extract	967:973	arg1	IC50					983:986	an IC50	980:986	an IC50 of 2.63 μg extract/ml	980:1008	The antioxidant activity was very high corresponding to 1567 mg Trolox equivalents/g of extract, and an IC50 of 2.63 μg extract/ml.
29763441	6	58	theme	extract	967:973	arg1	equivalents/g					950:962	1567 mg Trolox equivalents/g	935:962	1567 mg Trolox equivalents/g of extract	935:973	The antioxidant activity was very high corresponding to 1567 mg Trolox equivalents/g of extract, and an IC50 of 2.63 μg extract/ml.
29763441	6	59	theme	2.63	991:994	arg1	extract/ml					999:1008	2.63 μg extract/ml	991:1008	2.63 μg extract/ml	991:1008	The antioxidant activity was very high corresponding to 1567 mg Trolox equivalents/g of extract, and an IC50 of 2.63 μg extract/ml.
29763441	2	60	theme	following	246:254	arg1	%					277:277	the following composition: ash 14.6%	242:277	the following composition: ash 14.6%	242:277	The barks showed the following composition: ash 14.6%, total extractives 13.2%, suberin 2.9% and lignin 28.2%.
29763441	8	61	theme	specific	1500:1507	arg1	niches					1527:1532	specific high-value market niches	1500:1532	specific high-value market niches	1500:1532	In view of an integrated valorization, Quercus faginea barks are interesting sources of polar compounds including phenols and polyphenols with possible interesting bioactivities, while the sterols and triterpenes contained in the lipophilic extracts are also valuable bioactive compounds or chemical intermediates for specific high-value market niches, such as cosmetics, pharmaceuticals and biomedicine.
29763441	5	62	theme	GAE	752:754	arg1	/g					756:757	gallic acid equivalents (GAE)/g	727:757	gallic acid equivalents (GAE)/g of extract	727:768	Polar extracts (ethanol-water) had a high phenolic content of 630.3 mg of gallic acid equivalents (GAE)/g of extract, condensed tannins 220.7 mg of catechin equivalents (CE)/g extract, and flavonoids 207.7 mg CE/g of extract.
29763441	7	63	theme	alkanoic	1117:1124	arg1	acids					1126:1130	alkanoic acids	1117:1130	alkanoic acids (27.8%)	1117:1138	The lipophilic extracts were constituted mainly by glycerol and its derivatives (12.3% of all compounds), alkanoic acids (27.8%), sterols (11.5%) and triterpenes (17.8%).
29763441	7	63	theme	alkanoic	1117:1124	arg1	%					1137:1137	27.8%	1133:1137	27.8%	1133:1137	The lipophilic extracts were constituted mainly by glycerol and its derivatives (12.3% of all compounds), alkanoic acids (27.8%), sterols (11.5%) and triterpenes (17.8%).
29763441	4	64	theme	alkanols	599:606	arg1	%					611:611	alkanols 6.7%	599:611	alkanols 6.7%	599:611	The suberin composition was: ω-hydroxyacids 46.3% of total compounds, ɑ,ω-alkanoic diacids 22.3%, alkanoic acids 5.9%, alkanols 6.7% and aromatics 6.9% (ferulic acid 4.0%).
29763441	4	64	theme	alkanols	599:606	arg1	compounds					539:547	total compounds	533:547	total compounds	533:547	The suberin composition was: ω-hydroxyacids 46.3% of total compounds, ɑ,ω-alkanoic diacids 22.3%, alkanoic acids 5.9%, alkanols 6.7% and aromatics 6.9% (ferulic acid 4.0%).
29763441	5	65	theme	207.7	853:857	arg1	mg					859:860	mg	859:860	mg	859:860	Polar extracts (ethanol-water) had a high phenolic content of 630.3 mg of gallic acid equivalents (GAE)/g of extract, condensed tannins 220.7 mg of catechin equivalents (CE)/g extract, and flavonoids 207.7 mg CE/g of extract.
29763441	8	66	theme	market	1520:1525	arg1	niches					1527:1532	specific high-value market niches	1500:1532	specific high-value market niches	1500:1532	In view of an integrated valorization, Quercus faginea barks are interesting sources of polar compounds including phenols and polyphenols with possible interesting bioactivities, while the sterols and triterpenes contained in the lipophilic extracts are also valuable bioactive compounds or chemical intermediates for specific high-value market niches, such as cosmetics, pharmaceuticals and biomedicine.
29763441	5	67	theme	mg	721:722	arg1	content					704:710	a high phenolic content	688:710	a high phenolic content of 630.3 mg of gallic acid equivalents (GAE)/g of extract, condensed tannins 220.7 mg of catechin equivalents (CE)/g extract, and flavonoids 207.7 mg CE/g of extract	688:876	Polar extracts (ethanol-water) had a high phenolic content of 630.3 mg of gallic acid equivalents (GAE)/g of extract, condensed tannins 220.7 mg of catechin equivalents (CE)/g extract, and flavonoids 207.7 mg CE/g of extract.
29763441	8	68	dep	sterols	1371:1377	arg1	the					1367:1369	the	1367:1369	the	1367:1369	In view of an integrated valorization, Quercus faginea barks are interesting sources of polar compounds including phenols and polyphenols with possible interesting bioactivities, while the sterols and triterpenes contained in the lipophilic extracts are also valuable bioactive compounds or chemical intermediates for specific high-value market niches, such as cosmetics, pharmaceuticals and biomedicine.
29763441	1	69	theme	mature	148:153	arg1	trees					155:159	Quercus faginea mature trees	132:159	Quercus faginea mature trees from two sites	132:174	The bark from Quercus faginea mature trees from two sites was chemically characterized for the first time.
29763441	0	70	from	composition	9:19	arg1	faginea					43:49	Quercus faginea	35:49	Quercus faginea	35:49	Chemical composition of barks from Quercus faginea trees and characterization of their lipophilic and polar extracts.
29763441	4	71	theme	acids	587:591	arg1	compounds					539:547	total compounds	533:547	total compounds	533:547	The suberin composition was: ω-hydroxyacids 46.3% of total compounds, ɑ,ω-alkanoic diacids 22.3%, alkanoic acids 5.9%, alkanols 6.7% and aromatics 6.9% (ferulic acid 4.0%).
29763441	4	71	theme	acids	587:591	arg1	%					596:596	alkanoic acids 5.9%	578:596	alkanoic acids 5.9%	578:596	The suberin composition was: ω-hydroxyacids 46.3% of total compounds, ɑ,ω-alkanoic diacids 22.3%, alkanoic acids 5.9%, alkanols 6.7% and aromatics 6.9% (ferulic acid 4.0%).
29763441	1	72	from	trees	155:159	arg1	bark					122:125	The bark	118:125	The bark from Quercus faginea mature trees from two sites	118:174	The bark from Quercus faginea mature trees from two sites was chemically characterized for the first time.
29763441	5	73	theme	condensed	771:779	arg1	mg					795:796	condensed tannins 220.7 mg	771:796	condensed tannins 220.7 mg of catechin equivalents (CE)/g extract	771:835	Polar extracts (ethanol-water) had a high phenolic content of 630.3 mg of gallic acid equivalents (GAE)/g of extract, condensed tannins 220.7 mg of catechin equivalents (CE)/g extract, and flavonoids 207.7 mg CE/g of extract.
29763441	8	74	theme	Quercus	1221:1227	arg1	sources					1259:1265	interesting sources	1247:1265	interesting sources of polar compounds including phenols and polyphenols with possible interesting bioactivities	1247:1358	In view of an integrated valorization, Quercus faginea barks are interesting sources of polar compounds including phenols and polyphenols with possible interesting bioactivities, while the sterols and triterpenes contained in the lipophilic extracts are also valuable bioactive compounds or chemical intermediates for specific high-value market niches, such as cosmetics, pharmaceuticals and biomedicine.
29763441	8	74	theme	Quercus	1221:1227	arg1	barks					1237:1241	Quercus faginea barks	1221:1241	Quercus faginea barks	1221:1241	In view of an integrated valorization, Quercus faginea barks are interesting sources of polar compounds including phenols and polyphenols with possible interesting bioactivities, while the sterols and triterpenes contained in the lipophilic extracts are also valuable bioactive compounds or chemical intermediates for specific high-value market niches, such as cosmetics, pharmaceuticals and biomedicine.
29763441	0	75	theme	extracts	108:115	arg1	characterization					61:76	and characterization	57:76	characterization	61:76	Chemical composition of barks from Quercus faginea trees and characterization of their lipophilic and polar extracts.
29763441	4	76	theme	ferulic	633:639	arg1	acid					641:644	ferulic acid	633:644	ferulic acid 4.0%	633:649	The suberin composition was: ω-hydroxyacids 46.3% of total compounds, ɑ,ω-alkanoic diacids 22.3%, alkanoic acids 5.9%, alkanols 6.7% and aromatics 6.9% (ferulic acid 4.0%).
29763441	5	77	theme	Polar	653:657	arg1	extracts					659:666	Polar extracts	653:666	Polar extracts (ethanol-water)	653:682	Polar extracts (ethanol-water) had a high phenolic content of 630.3 mg of gallic acid equivalents (GAE)/g of extract, condensed tannins 220.7 mg of catechin equivalents (CE)/g extract, and flavonoids 207.7 mg CE/g of extract.
29763441	5	77	theme	Polar	653:657	arg1	ethanol-water					669:681	ethanol-water	669:681	ethanol-water	669:681	Polar extracts (ethanol-water) had a high phenolic content of 630.3 mg of gallic acid equivalents (GAE)/g of extract, condensed tannins 220.7 mg of catechin equivalents (CE)/g extract, and flavonoids 207.7 mg CE/g of extract.
29763441	5	78	theme	/g	756:757	arg1	flavonoids					842:851	flavonoids	842:851	flavonoids	842:851	Polar extracts (ethanol-water) had a high phenolic content of 630.3 mg of gallic acid equivalents (GAE)/g of extract, condensed tannins 220.7 mg of catechin equivalents (CE)/g extract, and flavonoids 207.7 mg CE/g of extract.
29763441	5	78	theme	/g	756:757	arg1	mg					721:722	630.3 mg	715:722	630.3 mg of gallic acid equivalents (GAE)/g of extract	715:768	Polar extracts (ethanol-water) had a high phenolic content of 630.3 mg of gallic acid equivalents (GAE)/g of extract, condensed tannins 220.7 mg of catechin equivalents (CE)/g extract, and flavonoids 207.7 mg CE/g of extract.
29763441	5	78	theme	/g	756:757	arg1	mg					795:796	condensed tannins 220.7 mg	771:796	condensed tannins 220.7 mg of catechin equivalents (CE)/g extract	771:835	Polar extracts (ethanol-water) had a high phenolic content of 630.3 mg of gallic acid equivalents (GAE)/g of extract, condensed tannins 220.7 mg of catechin equivalents (CE)/g extract, and flavonoids 207.7 mg CE/g of extract.
29763441	8	79	theme	interesting	1247:1257	arg1	sources					1259:1265	interesting sources	1247:1265	interesting sources of polar compounds including phenols and polyphenols with possible interesting bioactivities	1247:1358	In view of an integrated valorization, Quercus faginea barks are interesting sources of polar compounds including phenols and polyphenols with possible interesting bioactivities, while the sterols and triterpenes contained in the lipophilic extracts are also valuable bioactive compounds or chemical intermediates for specific high-value market niches, such as cosmetics, pharmaceuticals and biomedicine.
29763441	8	79	theme	interesting	1247:1257	arg1	barks					1237:1241	Quercus faginea barks	1221:1241	Quercus faginea barks	1221:1241	In view of an integrated valorization, Quercus faginea barks are interesting sources of polar compounds including phenols and polyphenols with possible interesting bioactivities, while the sterols and triterpenes contained in the lipophilic extracts are also valuable bioactive compounds or chemical intermediates for specific high-value market niches, such as cosmetics, pharmaceuticals and biomedicine.
29763441	5	80	contain	had	684:686	arg1	extracts					659:666	Polar extracts	653:666	Polar extracts (ethanol-water)	653:682	Polar extracts (ethanol-water) had a high phenolic content of 630.3 mg of gallic acid equivalents (GAE)/g of extract, condensed tannins 220.7 mg of catechin equivalents (CE)/g extract, and flavonoids 207.7 mg CE/g of extract.
29763441	5	80	contain	had	684:686	arg2	content					704:710	a high phenolic content	688:710	a high phenolic content of 630.3 mg of gallic acid equivalents (GAE)/g of extract, condensed tannins 220.7 mg of catechin equivalents (CE)/g extract, and flavonoids 207.7 mg CE/g of extract	688:876	Polar extracts (ethanol-water) had a high phenolic content of 630.3 mg of gallic acid equivalents (GAE)/g of extract, condensed tannins 220.7 mg of catechin equivalents (CE)/g extract, and flavonoids 207.7 mg CE/g of extract.
29763441	5	80	contain	had	684:686	arg1	ethanol-water					669:681	ethanol-water	669:681	ethanol-water	669:681	Polar extracts (ethanol-water) had a high phenolic content of 630.3 mg of gallic acid equivalents (GAE)/g of extract, condensed tannins 220.7 mg of catechin equivalents (CE)/g extract, and flavonoids 207.7 mg CE/g of extract.
29763441	0	81	theme	barks	24:28	arg1	composition					9:19	Chemical composition	0:19	Chemical composition of barks from Quercus faginea	0:49	Chemical composition of barks from Quercus faginea trees and characterization of their lipophilic and polar extracts.
29763441	5	82	theme	equivalents	810:820	arg1	flavonoids					842:851	flavonoids	842:851	flavonoids	842:851	Polar extracts (ethanol-water) had a high phenolic content of 630.3 mg of gallic acid equivalents (GAE)/g of extract, condensed tannins 220.7 mg of catechin equivalents (CE)/g extract, and flavonoids 207.7 mg CE/g of extract.
29763441	5	82	theme	equivalents	810:820	arg1	mg					721:722	630.3 mg	715:722	630.3 mg of gallic acid equivalents (GAE)/g of extract	715:768	Polar extracts (ethanol-water) had a high phenolic content of 630.3 mg of gallic acid equivalents (GAE)/g of extract, condensed tannins 220.7 mg of catechin equivalents (CE)/g extract, and flavonoids 207.7 mg CE/g of extract.
29763441	5	82	theme	equivalents	810:820	arg1	mg					795:796	condensed tannins 220.7 mg	771:796	condensed tannins 220.7 mg of catechin equivalents (CE)/g extract	771:835	Polar extracts (ethanol-water) had a high phenolic content of 630.3 mg of gallic acid equivalents (GAE)/g of extract, condensed tannins 220.7 mg of catechin equivalents (CE)/g extract, and flavonoids 207.7 mg CE/g of extract.
29763441	5	83	theme	extract	762:768	arg1	/g					756:757	gallic acid equivalents (GAE)/g	727:757	gallic acid equivalents (GAE)/g of extract	727:768	Polar extracts (ethanol-water) had a high phenolic content of 630.3 mg of gallic acid equivalents (GAE)/g of extract, condensed tannins 220.7 mg of catechin equivalents (CE)/g extract, and flavonoids 207.7 mg CE/g of extract.
29763441	4	84	theme	suberin	484:490	arg1	%					528:528	ω-hydroxyacids 46.3%	509:528	ω-hydroxyacids 46.3% of total compounds	509:547	The suberin composition was: ω-hydroxyacids 46.3% of total compounds, ɑ,ω-alkanoic diacids 22.3%, alkanoic acids 5.9%, alkanols 6.7% and aromatics 6.9% (ferulic acid 4.0%).
29763441	4	84	theme	suberin	484:490	arg1	composition					492:502	The suberin composition	480:502	The suberin composition	480:502	The suberin composition was: ω-hydroxyacids 46.3% of total compounds, ɑ,ω-alkanoic diacids 22.3%, alkanoic acids 5.9%, alkanols 6.7% and aromatics 6.9% (ferulic acid 4.0%).
29763441	4	84	theme	suberin	484:490	arg1	compounds					539:547	total compounds	533:547	total compounds	533:547	The suberin composition was: ω-hydroxyacids 46.3% of total compounds, ɑ,ω-alkanoic diacids 22.3%, alkanoic acids 5.9%, alkanols 6.7% and aromatics 6.9% (ferulic acid 4.0%).
29763441	4	85	theme	aromatics	617:625	arg1	compounds					539:547	total compounds	533:547	total compounds	533:547	The suberin composition was: ω-hydroxyacids 46.3% of total compounds, ɑ,ω-alkanoic diacids 22.3%, alkanoic acids 5.9%, alkanols 6.7% and aromatics 6.9% (ferulic acid 4.0%).
29763441	4	85	theme	aromatics	617:625	arg1	%					630:630	aromatics 6.9%	617:630	aromatics 6.9% (ferulic acid 4.0%)	617:650	The suberin composition was: ω-hydroxyacids 46.3% of total compounds, ɑ,ω-alkanoic diacids 22.3%, alkanoic acids 5.9%, alkanols 6.7% and aromatics 6.9% (ferulic acid 4.0%).
29763441	0	86	theme	Quercus	35:41	arg1	faginea					43:49	Quercus faginea	35:49	Quercus faginea	35:49	Chemical composition of barks from Quercus faginea trees and characterization of their lipophilic and polar extracts.
29763441	8	87	theme	compounds	1276:1284	arg1	sources					1259:1265	interesting sources	1247:1265	interesting sources of polar compounds including phenols and polyphenols with possible interesting bioactivities	1247:1358	In view of an integrated valorization, Quercus faginea barks are interesting sources of polar compounds including phenols and polyphenols with possible interesting bioactivities, while the sterols and triterpenes contained in the lipophilic extracts are also valuable bioactive compounds or chemical intermediates for specific high-value market niches, such as cosmetics, pharmaceuticals and biomedicine.
29763441	8	87	theme	compounds	1276:1284	arg1	barks					1237:1241	Quercus faginea barks	1221:1241	Quercus faginea barks	1221:1241	In view of an integrated valorization, Quercus faginea barks are interesting sources of polar compounds including phenols and polyphenols with possible interesting bioactivities, while the sterols and triterpenes contained in the lipophilic extracts are also valuable bioactive compounds or chemical intermediates for specific high-value market niches, such as cosmetics, pharmaceuticals and biomedicine.
29763441	4	88	theme	total	533:537	arg1	compounds					539:547	total compounds	533:547	total compounds	533:547	The suberin composition was: ω-hydroxyacids 46.3% of total compounds, ɑ,ω-alkanoic diacids 22.3%, alkanoic acids 5.9%, alkanols 6.7% and aromatics 6.9% (ferulic acid 4.0%).
29763441	4	89	theme	4.0	646:648	arg1	%					649:649	%	649:649	%	649:649	The suberin composition was: ω-hydroxyacids 46.3% of total compounds, ɑ,ω-alkanoic diacids 22.3%, alkanoic acids 5.9%, alkanols 6.7% and aromatics 6.9% (ferulic acid 4.0%).
29763441	7	90	dep	derivatives	1079:1089	arg1	%					1096:1096	12.3%	1092:1096	12.3% of all compounds	1092:1113	The lipophilic extracts were constituted mainly by glycerol and its derivatives (12.3% of all compounds), alkanoic acids (27.8%), sterols (11.5%) and triterpenes (17.8%).
29763441	7	90	dep	derivatives	1079:1089	arg1	compounds					1105:1113	all compounds	1101:1113	all compounds	1101:1113	The lipophilic extracts were constituted mainly by glycerol and its derivatives (12.3% of all compounds), alkanoic acids (27.8%), sterols (11.5%) and triterpenes (17.8%).
29763441	8	91	theme	possible	1325:1332	arg1	bioactivities					1346:1358	possible interesting bioactivities	1325:1358	possible interesting bioactivities	1325:1358	In view of an integrated valorization, Quercus faginea barks are interesting sources of polar compounds including phenols and polyphenols with possible interesting bioactivities, while the sterols and triterpenes contained in the lipophilic extracts are also valuable bioactive compounds or chemical intermediates for specific high-value market niches, such as cosmetics, pharmaceuticals and biomedicine.
30123851	0	0	theme	capsule	76:82	arg1	composition					84:94	capsule composition	76:94	capsule composition	76:94	Titan cell production in Cryptococcus neoformans reshapes the cell wall and capsule composition during infection.
30123851	0	1	from	production	11:20	arg1	neoformans					38:47	Cryptococcus neoformans	25:47	Cryptococcus neoformans	25:47	Titan cell production in Cryptococcus neoformans reshapes the cell wall and capsule composition during infection.
30123851	5	2	theme	capsule	652:658	arg1	composition					660:670	capsule composition	652:670	capsule composition	652:670	Here we examined the cell wall and capsule composition of in vitro, in vivo typical-sized and in vivo titan cells using High Performance Liquid Chromatography (HPLC).
30123851	9	3	theme	capsule	1359:1365	arg1	composition					1367:1377	capsule composition	1359:1377	capsule composition	1359:1377	Taken together, these results demonstrate that C. neoformans reshapes its cell wall and capsule composition during infection.
30123851	5	4	dep	in	685:686	arg1	vivo					688:691	vivo	688:691	vivo	688:691	Here we examined the cell wall and capsule composition of in vitro, in vivo typical-sized and in vivo titan cells using High Performance Liquid Chromatography (HPLC).
30123851	5	4	dep	in	685:686	arg1	typical-sized					693:705	typical-sized	693:705	typical-sized	693:705	Here we examined the cell wall and capsule composition of in vitro, in vivo typical-sized and in vivo titan cells using High Performance Liquid Chromatography (HPLC).
30123851	4	5	theme	anti-inflammatory	582:598	arg1	response					607:614	a detrimental anti-inflammatory immune response	568:614	a detrimental anti-inflammatory immune response	568:614	In addition, titan cells have increased cell wall chitin that is associated with a detrimental anti-inflammatory immune response.
30123851	4	6	theme	titan	500:504	arg1	cells					506:510	titan cells	500:510	titan cells	500:510	In addition, titan cells have increased cell wall chitin that is associated with a detrimental anti-inflammatory immune response.
30123851	4	7	theme	increased	517:525	arg1	chitin					537:542	increased cell wall chitin	517:542	increased cell wall chitin that is associated with a detrimental anti-inflammatory immune response	517:614	In addition, titan cells have increased cell wall chitin that is associated with a detrimental anti-inflammatory immune response.
30123851	10	8	theme	capsule	1417:1423	arg1	alterations					1425:1435	These cell wall and capsule alterations	1397:1435	alterations	1425:1435	These cell wall and capsule alterations likely help C. neoformans escape recognition by, and allow modulation of, the host immune system.
30123851	6	9	theme	cell	811:814	arg1	polysaccharides					821:835	cell wall polysaccharides	811:835	cell wall polysaccharides	811:835	The monomer composition of cell wall polysaccharides showed that in vivo C. neoformans cells contained more glucosamine and less glucose than in vitro cells, suggesting alteration in abundance of both chitin and glucans, respectively.
30123851	2	10	theme	cells	369:373	arg1	production					343:352	production	343:352	production of large titan cells	343:373	Upon inhalation into the lungs C. neoformans differentiates into cells with altered size and morphology, including production of large titan cells.
30123851	10	11	theme	system	1527:1532	arg1	modulation					1496:1505	modulation	1496:1505	modulation of, the host immune system	1496:1532	These cell wall and capsule alterations likely help C. neoformans escape recognition by, and allow modulation of, the host immune system.
30123851	4	12	theme	detrimental	570:580	arg1	response					607:614	a detrimental anti-inflammatory immune response	568:614	a detrimental anti-inflammatory immune response	568:614	In addition, titan cells have increased cell wall chitin that is associated with a detrimental anti-inflammatory immune response.
30123851	3	13	theme	Titan	376:380	arg1	cells					382:386	Titan cells	376:386	Titan cells	376:386	Titan cells possess thickened cell wall and dense, cross-linked capsule when compared to in vitro grown cells.
30123851	8	14	from	differences	1154:1164	arg1	proportions					1173:1183	the proportions	1169:1183	the proportions of cell wall and capsule monomers	1169:1217	Within the in vivo cell population, differences in the proportions of cell wall and capsule monomers between typical and titan cells were also observed.
30123851	10	15	theme	immune	1520:1525	arg1	system					1527:1532	, the host immune system	1509:1532	system	1527:1532	These cell wall and capsule alterations likely help C. neoformans escape recognition by, and allow modulation of, the host immune system.
30123851	8	16	theme	wall	1193:1196	arg1	proportions					1173:1183	the proportions	1169:1183	the proportions of cell wall and capsule monomers	1169:1217	Within the in vivo cell population, differences in the proportions of cell wall and capsule monomers between typical and titan cells were also observed.
30123851	5	17	theme	cells	725:729	arg1	wall					643:646	cell wall	638:646	cell wall	638:646	Here we examined the cell wall and capsule composition of in vitro, in vivo typical-sized and in vivo titan cells using High Performance Liquid Chromatography (HPLC).
30123851	5	17	theme	cells	725:729	arg1	composition					660:670	capsule composition	652:670	capsule composition	652:670	Here we examined the cell wall and capsule composition of in vitro, in vivo typical-sized and in vivo titan cells using High Performance Liquid Chromatography (HPLC).
30123851	5	18	theme	in	675:676	arg1	cells					725:729	in vitro, in vivo typical-sized and in vivo titan cells	675:729	in vitro, in vivo typical-sized and in vivo titan cells	675:729	Here we examined the cell wall and capsule composition of in vitro, in vivo typical-sized and in vivo titan cells using High Performance Liquid Chromatography (HPLC).
30123851	2	19	theme	altered	304:310	arg1	size					312:315	altered size	304:315	altered size	304:315	Upon inhalation into the lungs C. neoformans differentiates into cells with altered size and morphology, including production of large titan cells.
30123851	6	20	dep	less	908:911	arg1	glucose					913:919	glucose	913:919	glucose	913:919	The monomer composition of cell wall polysaccharides showed that in vivo C. neoformans cells contained more glucosamine and less glucose than in vitro cells, suggesting alteration in abundance of both chitin and glucans, respectively.
30123851	4	21	theme	immune	600:605	arg1	response					607:614	a detrimental anti-inflammatory immune response	568:614	a detrimental anti-inflammatory immune response	568:614	In addition, titan cells have increased cell wall chitin that is associated with a detrimental anti-inflammatory immune response.
30123851	1	22	theme	Cryptococcus	114:125	arg1	pathogen					156:163	a human fungal pathogen	141:163	a human fungal pathogen that often causes infections in immunocompromised individuals	141:225	Cryptococcus neoformans is a human fungal pathogen that often causes infections in immunocompromised individuals.
30123851	1	22	theme	Cryptococcus	114:125	arg1	neoformans					127:136	Cryptococcus neoformans	114:136	Cryptococcus neoformans	114:136	Cryptococcus neoformans is a human fungal pathogen that often causes infections in immunocompromised individuals.
30123851	8	23	dep	in	1129:1130	arg1	vivo					1132:1135	vivo	1132:1135	vivo	1132:1135	Within the in vivo cell population, differences in the proportions of cell wall and capsule monomers between typical and titan cells were also observed.
30123851	6	24	theme	monomer	788:794	arg1	composition					796:806	The monomer composition	784:806	The monomer composition of cell wall polysaccharides	784:835	The monomer composition of cell wall polysaccharides showed that in vivo C. neoformans cells contained more glucosamine and less glucose than in vitro cells, suggesting alteration in abundance of both chitin and glucans, respectively.
30123851	8	25	theme	titan	1239:1243	arg1	cells					1245:1249	typical and titan cells	1227:1249	typical and titan cells	1227:1249	Within the in vivo cell population, differences in the proportions of cell wall and capsule monomers between typical and titan cells were also observed.
30123851	8	26	theme	typical	1227:1233	arg1	cells					1245:1249	typical and titan cells	1227:1249	typical and titan cells	1227:1249	Within the in vivo cell population, differences in the proportions of cell wall and capsule monomers between typical and titan cells were also observed.
30123851	8	27	theme	monomers	1210:1217	arg1	proportions					1173:1183	the proportions	1169:1183	the proportions of cell wall and capsule monomers	1169:1217	Within the in vivo cell population, differences in the proportions of cell wall and capsule monomers between typical and titan cells were also observed.
30123851	5	28	theme	High	737:740	arg1	HPLC					777:780	HPLC	777:780	HPLC	777:780	Here we examined the cell wall and capsule composition of in vitro, in vivo typical-sized and in vivo titan cells using High Performance Liquid Chromatography (HPLC).
30123851	5	28	theme	High	737:740	arg1	Chromatography					761:774	High Performance Liquid Chromatography	737:774	High Performance Liquid Chromatography (HPLC)	737:781	Here we examined the cell wall and capsule composition of in vitro, in vivo typical-sized and in vivo titan cells using High Performance Liquid Chromatography (HPLC).
30123851	3	29	dep	dense	420:424	arg1	cross-linked					427:438	cross-linked	427:438	cross-linked	427:438	Titan cells possess thickened cell wall and dense, cross-linked capsule when compared to in vitro grown cells.
30123851	6	30	theme	in	849:850	arg1	cells					871:875	in vivo C. neoformans cells	849:875	in vivo C. neoformans cells	849:875	The monomer composition of cell wall polysaccharides showed that in vivo C. neoformans cells contained more glucosamine and less glucose than in vitro cells, suggesting alteration in abundance of both chitin and glucans, respectively.
30123851	5	31	dep	in	675:676	arg1	vitro					678:682	vitro	678:682	vitro	678:682	Here we examined the cell wall and capsule composition of in vitro, in vivo typical-sized and in vivo titan cells using High Performance Liquid Chromatography (HPLC).
30123851	8	32	theme	capsule	1202:1208	arg1	monomers					1210:1217	capsule monomers	1202:1217	capsule monomers	1202:1217	Within the in vivo cell population, differences in the proportions of cell wall and capsule monomers between typical and titan cells were also observed.
30123851	3	33	theme	cell	406:409	arg1	wall					411:414	thickened cell wall	396:414	thickened cell wall	396:414	Titan cells possess thickened cell wall and dense, cross-linked capsule when compared to in vitro grown cells.
30123851	10	34	theme	cell	1403:1406	arg1	wall					1408:1411	These cell wall and capsule alterations	1397:1435	wall	1408:1411	These cell wall and capsule alterations likely help C. neoformans escape recognition by, and allow modulation of, the host immune system.
30123851	3	35	contain	possess	388:394	arg2	capsule					440:446	dense, cross-linked capsule	420:446	dense, cross-linked capsule	420:446	Titan cells possess thickened cell wall and dense, cross-linked capsule when compared to in vitro grown cells.
30123851	3	35	contain	possess	388:394	arg2	wall					411:414	thickened cell wall	396:414	thickened cell wall	396:414	Titan cells possess thickened cell wall and dense, cross-linked capsule when compared to in vitro grown cells.
30123851	3	35	contain	possess	388:394	arg1	cells					382:386	Titan cells	376:386	Titan cells	376:386	Titan cells possess thickened cell wall and dense, cross-linked capsule when compared to in vitro grown cells.
30123851	7	36	theme	vitro	1105:1109	arg1	cells					1111:1115	both in vivo and vitro cells	1088:1115	both in vivo and vitro cells	1088:1115	Low levels of galactosamine were also detected in carbohydrates from both in vivo and vitro cells.
30123851	0	37	theme	Titan	0:4	arg1	production					11:20	Titan cell production	0:20	Titan cell production in Cryptococcus neoformans	0:47	Titan cell production in Cryptococcus neoformans reshapes the cell wall and capsule composition during infection.
30123851	8	38	theme	in	1129:1130	arg1	population					1142:1151	the in vivo cell population	1125:1151	the in vivo cell population	1125:1151	Within the in vivo cell population, differences in the proportions of cell wall and capsule monomers between typical and titan cells were also observed.
30123851	5	39	theme	titan	719:723	arg1	cells					725:729	in vitro, in vivo typical-sized and in vivo titan cells	675:729	in vitro, in vivo typical-sized and in vivo titan cells	675:729	Here we examined the cell wall and capsule composition of in vitro, in vivo typical-sized and in vivo titan cells using High Performance Liquid Chromatography (HPLC).
30123851	6	40	dep	in	926:927	arg1	vitro					929:933	vitro	929:933	vitro	929:933	The monomer composition of cell wall polysaccharides showed that in vivo C. neoformans cells contained more glucosamine and less glucose than in vitro cells, suggesting alteration in abundance of both chitin and glucans, respectively.
30123851	6	41	from	alteration	953:962	arg1	abundance					967:975	abundance	967:975	abundance of both chitin and glucans, respectively	967:1016	The monomer composition of cell wall polysaccharides showed that in vivo C. neoformans cells contained more glucosamine and less glucose than in vitro cells, suggesting alteration in abundance of both chitin and glucans, respectively.
30123851	8	42	theme	cell	1188:1191	arg1	wall					1193:1196	cell wall	1188:1196	cell wall	1188:1196	Within the in vivo cell population, differences in the proportions of cell wall and capsule monomers between typical and titan cells were also observed.
30123851	7	43	located	detected	1057:1064	arg1	carbohydrates					1069:1081	carbohydrates	1069:1081	carbohydrates from both in vivo and vitro cells	1069:1115	Low levels of galactosamine were also detected in carbohydrates from both in vivo and vitro cells.
30123851	7	43	located	detected	1057:1064	arg2	levels					1023:1028	Low levels	1019:1028	Low levels of galactosamine	1019:1045	Low levels of galactosamine were also detected in carbohydrates from both in vivo and vitro cells.
30123851	3	44	theme	grown	474:478	arg1	cells					480:484	in vitro grown cells	465:484	in vitro grown cells	465:484	Titan cells possess thickened cell wall and dense, cross-linked capsule when compared to in vitro grown cells.
30123851	3	45	theme	thickened	396:404	arg1	wall					411:414	thickened cell wall	396:414	thickened cell wall	396:414	Titan cells possess thickened cell wall and dense, cross-linked capsule when compared to in vitro grown cells.
30123851	8	46	located	observed	1261:1268	arg2	differences					1154:1164	differences	1154:1164	differences in the proportions of cell wall and capsule monomers between typical and titan cells	1154:1249	Within the in vivo cell population, differences in the proportions of cell wall and capsule monomers between typical and titan cells were also observed.
30123851	8	46	located	observed	1261:1268	arg1	population					1142:1151	the in vivo cell population	1125:1151	the in vivo cell population	1125:1151	Within the in vivo cell population, differences in the proportions of cell wall and capsule monomers between typical and titan cells were also observed.
30123851	6	47	theme	neoformans	860:869	arg1	cells					871:875	in vivo C. neoformans cells	849:875	in vivo C. neoformans cells	849:875	The monomer composition of cell wall polysaccharides showed that in vivo C. neoformans cells contained more glucosamine and less glucose than in vitro cells, suggesting alteration in abundance of both chitin and glucans, respectively.
30123851	10	48	theme	host	1515:1518	arg1	system					1527:1532	, the host immune system	1509:1532	system	1527:1532	These cell wall and capsule alterations likely help C. neoformans escape recognition by, and allow modulation of, the host immune system.
30123851	4	49	contain	have	512:515	arg1	cells					506:510	titan cells	500:510	titan cells	500:510	In addition, titan cells have increased cell wall chitin that is associated with a detrimental anti-inflammatory immune response.
30123851	4	49	contain	have	512:515	arg2	chitin					537:542	increased cell wall chitin	517:542	increased cell wall chitin that is associated with a detrimental anti-inflammatory immune response	517:614	In addition, titan cells have increased cell wall chitin that is associated with a detrimental anti-inflammatory immune response.
30123851	5	50	dep	wall	643:646	arg1	the					634:636	the	634:636	the	634:636	Here we examined the cell wall and capsule composition of in vitro, in vivo typical-sized and in vivo titan cells using High Performance Liquid Chromatography (HPLC).
30123851	5	51	theme	in	711:712	arg1	cells					725:729	in vitro, in vivo typical-sized and in vivo titan cells	675:729	in vitro, in vivo typical-sized and in vivo titan cells	675:729	Here we examined the cell wall and capsule composition of in vitro, in vivo typical-sized and in vivo titan cells using High Performance Liquid Chromatography (HPLC).
30123851	6	52	theme	C.	857:858	arg1	cells					871:875	in vivo C. neoformans cells	849:875	in vivo C. neoformans cells	849:875	The monomer composition of cell wall polysaccharides showed that in vivo C. neoformans cells contained more glucosamine and less glucose than in vitro cells, suggesting alteration in abundance of both chitin and glucans, respectively.
30123851	0	53	theme	Cryptococcus	25:36	arg1	neoformans					38:47	Cryptococcus neoformans	25:47	Cryptococcus neoformans	25:47	Titan cell production in Cryptococcus neoformans reshapes the cell wall and capsule composition during infection.
30123851	4	54	theme	wall	532:535	arg1	chitin					537:542	increased cell wall chitin	517:542	increased cell wall chitin that is associated with a detrimental anti-inflammatory immune response	517:614	In addition, titan cells have increased cell wall chitin that is associated with a detrimental anti-inflammatory immune response.
30123851	6	55	theme	chitin	985:990	arg1	abundance					967:975	abundance	967:975	abundance of both chitin and glucans, respectively	967:1016	The monomer composition of cell wall polysaccharides showed that in vivo C. neoformans cells contained more glucosamine and less glucose than in vitro cells, suggesting alteration in abundance of both chitin and glucans, respectively.
30123851	6	56	theme	vivo	852:855	arg1	cells					871:875	in vivo C. neoformans cells	849:875	in vivo C. neoformans cells	849:875	The monomer composition of cell wall polysaccharides showed that in vivo C. neoformans cells contained more glucosamine and less glucose than in vitro cells, suggesting alteration in abundance of both chitin and glucans, respectively.
30123851	7	57	from	cells	1111:1115	arg1	carbohydrates					1069:1081	carbohydrates	1069:1081	carbohydrates from both in vivo and vitro cells	1069:1115	Low levels of galactosamine were also detected in carbohydrates from both in vivo and vitro cells.
30123851	4	58	theme	cell	527:530	arg1	chitin					537:542	increased cell wall chitin	517:542	increased cell wall chitin that is associated with a detrimental anti-inflammatory immune response	517:614	In addition, titan cells have increased cell wall chitin that is associated with a detrimental anti-inflammatory immune response.
30123851	5	59	theme	Liquid	754:759	arg1	HPLC					777:780	HPLC	777:780	HPLC	777:780	Here we examined the cell wall and capsule composition of in vitro, in vivo typical-sized and in vivo titan cells using High Performance Liquid Chromatography (HPLC).
30123851	5	59	theme	Liquid	754:759	arg1	Chromatography					761:774	High Performance Liquid Chromatography	737:774	High Performance Liquid Chromatography (HPLC)	737:781	Here we examined the cell wall and capsule composition of in vitro, in vivo typical-sized and in vivo titan cells using High Performance Liquid Chromatography (HPLC).
30123851	7	60	theme	Low	1019:1021	arg1	levels					1023:1028	Low levels	1019:1028	Low levels of galactosamine	1019:1045	Low levels of galactosamine were also detected in carbohydrates from both in vivo and vitro cells.
30123851	2	61	theme	titan	363:367	arg1	cells					369:373	large titan cells	357:373	large titan cells	357:373	Upon inhalation into the lungs C. neoformans differentiates into cells with altered size and morphology, including production of large titan cells.
30123851	3	62	theme	dense	420:424	arg1	capsule					440:446	dense, cross-linked capsule	420:446	dense, cross-linked capsule	420:446	Titan cells possess thickened cell wall and dense, cross-linked capsule when compared to in vitro grown cells.
30123851	9	63	theme	cell	1345:1348	arg1	wall					1350:1353	its cell wall	1341:1353	its cell wall	1341:1353	Taken together, these results demonstrate that C. neoformans reshapes its cell wall and capsule composition during infection.
30123851	7	64	theme	galactosamine	1033:1045	arg1	levels					1023:1028	Low levels	1019:1028	Low levels of galactosamine	1019:1045	Low levels of galactosamine were also detected in carbohydrates from both in vivo and vitro cells.
30123851	5	65	theme	cell	638:641	arg1	wall					643:646	cell wall	638:646	cell wall	638:646	Here we examined the cell wall and capsule composition of in vitro, in vivo typical-sized and in vivo titan cells using High Performance Liquid Chromatography (HPLC).
30123851	2	66	theme	large	357:361	arg1	cells					369:373	large titan cells	357:373	large titan cells	357:373	Upon inhalation into the lungs C. neoformans differentiates into cells with altered size and morphology, including production of large titan cells.
30123851	6	67	theme	in	926:927	arg1	cells					935:939	in vitro cells	926:939	in vitro cells	926:939	The monomer composition of cell wall polysaccharides showed that in vivo C. neoformans cells contained more glucosamine and less glucose than in vitro cells, suggesting alteration in abundance of both chitin and glucans, respectively.
30123851	5	68	theme	in	685:686	arg1	cells					725:729	in vitro, in vivo typical-sized and in vivo titan cells	675:729	in vitro, in vivo typical-sized and in vivo titan cells	675:729	Here we examined the cell wall and capsule composition of in vitro, in vivo typical-sized and in vivo titan cells using High Performance Liquid Chromatography (HPLC).
30123851	5	69	dep	in	711:712	arg1	vivo					714:717	vivo	714:717	vivo	714:717	Here we examined the cell wall and capsule composition of in vitro, in vivo typical-sized and in vivo titan cells using High Performance Liquid Chromatography (HPLC).
30123851	8	70	theme	cell	1137:1140	arg1	population					1142:1151	the in vivo cell population	1125:1151	the in vivo cell population	1125:1151	Within the in vivo cell population, differences in the proportions of cell wall and capsule monomers between typical and titan cells were also observed.
30123851	6	71	theme	polysaccharides	821:835	arg1	composition					796:806	The monomer composition	784:806	The monomer composition of cell wall polysaccharides	784:835	The monomer composition of cell wall polysaccharides showed that in vivo C. neoformans cells contained more glucosamine and less glucose than in vitro cells, suggesting alteration in abundance of both chitin and glucans, respectively.
30123851	7	72	theme	in	1093:1094	arg1	cells					1111:1115	both in vivo and vitro cells	1088:1115	both in vivo and vitro cells	1088:1115	Low levels of galactosamine were also detected in carbohydrates from both in vivo and vitro cells.
30123851	1	73	theme	fungal	149:154	arg1	pathogen					156:163	a human fungal pathogen	141:163	a human fungal pathogen that often causes infections in immunocompromised individuals	141:225	Cryptococcus neoformans is a human fungal pathogen that often causes infections in immunocompromised individuals.
30123851	1	73	theme	fungal	149:154	arg1	neoformans					127:136	Cryptococcus neoformans	114:136	Cryptococcus neoformans	114:136	Cryptococcus neoformans is a human fungal pathogen that often causes infections in immunocompromised individuals.
30123851	2	74	with	cells	293:297	arg1	morphology					321:330	morphology	321:330	morphology	321:330	Upon inhalation into the lungs C. neoformans differentiates into cells with altered size and morphology, including production of large titan cells.
30123851	2	74	with	cells	293:297	arg1	size					312:315	altered size	304:315	altered size	304:315	Upon inhalation into the lungs C. neoformans differentiates into cells with altered size and morphology, including production of large titan cells.
30123851	5	75	theme	Performance	742:752	arg1	HPLC					777:780	HPLC	777:780	HPLC	777:780	Here we examined the cell wall and capsule composition of in vitro, in vivo typical-sized and in vivo titan cells using High Performance Liquid Chromatography (HPLC).
30123851	5	75	theme	Performance	742:752	arg1	Chromatography					761:774	High Performance Liquid Chromatography	737:774	High Performance Liquid Chromatography (HPLC)	737:781	Here we examined the cell wall and capsule composition of in vitro, in vivo typical-sized and in vivo titan cells using High Performance Liquid Chromatography (HPLC).
30123851	6	76	theme	wall	816:819	arg1	polysaccharides					821:835	cell wall polysaccharides	811:835	cell wall polysaccharides	811:835	The monomer composition of cell wall polysaccharides showed that in vivo C. neoformans cells contained more glucosamine and less glucose than in vitro cells, suggesting alteration in abundance of both chitin and glucans, respectively.
30123851	1	77	theme	immunocompromised	197:213	arg1	individuals					215:225	immunocompromised individuals	197:225	immunocompromised individuals	197:225	Cryptococcus neoformans is a human fungal pathogen that often causes infections in immunocompromised individuals.
30123851	1	78	theme	human	143:147	arg1	pathogen					156:163	a human fungal pathogen	141:163	a human fungal pathogen that often causes infections in immunocompromised individuals	141:225	Cryptococcus neoformans is a human fungal pathogen that often causes infections in immunocompromised individuals.
30123851	1	78	theme	human	143:147	arg1	neoformans					127:136	Cryptococcus neoformans	114:136	Cryptococcus neoformans	114:136	Cryptococcus neoformans is a human fungal pathogen that often causes infections in immunocompromised individuals.
30123851	7	79	dep	in	1093:1094	arg1	vivo					1096:1099	vivo	1096:1099	vivo	1096:1099	Low levels of galactosamine were also detected in carbohydrates from both in vivo and vitro cells.
30123851	0	80	theme	cell	62:65	arg1	wall					67:70	the cell wall	58:70	the cell wall	58:70	Titan cell production in Cryptococcus neoformans reshapes the cell wall and capsule composition during infection.
30123851	6	81	dep	more	887:890	arg1	glucosamine					892:902	glucosamine	892:902	glucosamine	892:902	The monomer composition of cell wall polysaccharides showed that in vivo C. neoformans cells contained more glucosamine and less glucose than in vitro cells, suggesting alteration in abundance of both chitin and glucans, respectively.
30123851	0	82	theme	cell	6:9	arg1	production					11:20	Titan cell production	0:20	Titan cell production in Cryptococcus neoformans	0:47	Titan cell production in Cryptococcus neoformans reshapes the cell wall and capsule composition during infection.
30123851	6	83	theme	glucans	996:1002	arg1	abundance					967:975	abundance	967:975	abundance of both chitin and glucans, respectively	967:1016	The monomer composition of cell wall polysaccharides showed that in vivo C. neoformans cells contained more glucosamine and less glucose than in vitro cells, suggesting alteration in abundance of both chitin and glucans, respectively.
31731975	0	0	theme	solvent	97:103	arg1	stability					105:113	organic solvent stability	89:113	organic solvent stability	89:113	N-linked glycosylation of thermostable lipase from Bacillus thermocatenulatus to improve organic solvent stability.
31731975	3	1	from	45 °C	621:625	arg1	incubation					607:616	incubation	607:616	incubation at 45 °C	607:625	Among the six mutants, glycosylated A277 N and T236 N showed higher stability in the presence of 25% (v/v) DMSO (74.3 and 72.8% of initial activity was remained after incubation at 45 °C for 20 h, respectively) than deglycosylated A277 N and T236 N (57.2 and 45.1% of initial activity was remained, respectively).
31731975	4	2	theme	remaining	800:808	arg1	activity					810:817	higher remaining activity	793:817	higher remaining activity than wild-type BTL2	793:837	These glycosylated mutants also showed higher remaining activity than wild-type BTL2 (56.0% of the initial activity were remained).
31731975	5	3	theme	glycol	993:998	arg1	presence					962:969	the presence	958:969	the presence of 25% (v/v) ethylene glycol, DMSO, and DMF	958:1013	Furthermore, the glycosylated mutant T236 N showed longer half-lives in the presence of 25% (v/v) ethylene glycol, DMSO, and DMF (161, 133, and 56.7 h at 45 °C, respectively) than deglycosylated mutant T236 N (107, 91.9, and 42.8 h, respectively).
31731975	0	4	theme	organic	89:95	arg1	stability					105:113	organic solvent stability	89:113	organic solvent stability	89:113	N-linked glycosylation of thermostable lipase from Bacillus thermocatenulatus to improve organic solvent stability.
31731975	0	5	from	glycosylation	9:21	arg1	thermocatenulatus					60:76	Bacillus thermocatenulatus	51:76	Bacillus thermocatenulatus	51:76	N-linked glycosylation of thermostable lipase from Bacillus thermocatenulatus to improve organic solvent stability.
31731975	1	6	link	N-linked	235:242	arg1	glycosylation					244:256	N-linked glycosylation	235:256	N-linked glycosylation	235:256	A thermostable lipase from Bacillus thermocatenulatus was glycosylated by forming the consensus sequence (-NXS/T-) for N-linked glycosylation by site-directed mutagenesis.
31731975	3	7	theme	higher	501:506	arg1	stability					508:516	higher stability	501:516	higher stability	501:516	Among the six mutants, glycosylated A277 N and T236 N showed higher stability in the presence of 25% (v/v) DMSO (74.3 and 72.8% of initial activity was remained after incubation at 45 °C for 20 h, respectively) than deglycosylated A277 N and T236 N (57.2 and 45.1% of initial activity was remained, respectively).
31731975	5	8	dep	%	976:976	arg1	v/v					979:981	v/v	979:981	v/v	979:981	Furthermore, the glycosylated mutant T236 N showed longer half-lives in the presence of 25% (v/v) ethylene glycol, DMSO, and DMF (161, 133, and 56.7 h at 45 °C, respectively) than deglycosylated mutant T236 N (107, 91.9, and 42.8 h, respectively).
31731975	5	9	theme	mutant	916:921	arg1	T236 N					923:928	the glycosylated mutant T236 N	899:928	the glycosylated mutant T236 N	899:928	Furthermore, the glycosylated mutant T236 N showed longer half-lives in the presence of 25% (v/v) ethylene glycol, DMSO, and DMF (161, 133, and 56.7 h at 45 °C, respectively) than deglycosylated mutant T236 N (107, 91.9, and 42.8 h, respectively).
31731975	1	10	theme	thermostable	118:129	arg1	lipase					131:136	A thermostable lipase	116:136	A thermostable lipase from Bacillus thermocatenulatus	116:168	A thermostable lipase from Bacillus thermocatenulatus was glycosylated by forming the consensus sequence (-NXS/T-) for N-linked glycosylation by site-directed mutagenesis.
31731975	4	11	theme	activity	861:868	arg1	activity					861:868	the initial activity	849:868	the initial activity	849:868	These glycosylated mutants also showed higher remaining activity than wild-type BTL2 (56.0% of the initial activity were remained).
31731975	4	11	theme	activity	861:868	arg1	%					844:844	56.0%	840:844	56.0% of the initial activity	840:868	These glycosylated mutants also showed higher remaining activity than wild-type BTL2 (56.0% of the initial activity were remained).
31731975	0	12	from	thermocatenulatus	60:76	arg1	glycosylation					9:21	N-linked glycosylation	0:21	N-linked glycosylation of thermostable lipase from Bacillus thermocatenulatus	0:76	N-linked glycosylation of thermostable lipase from Bacillus thermocatenulatus to improve organic solvent stability.
31731975	0	12	from	thermocatenulatus	60:76	arg1	lipase					39:44	thermostable lipase	26:44	thermostable lipase from Bacillus thermocatenulatus	26:76	N-linked glycosylation of thermostable lipase from Bacillus thermocatenulatus to improve organic solvent stability.
31731975	3	13	theme	25	537:538	arg1	%					539:539	%	539:539	%	539:539	Among the six mutants, glycosylated A277 N and T236 N showed higher stability in the presence of 25% (v/v) DMSO (74.3 and 72.8% of initial activity was remained after incubation at 45 °C for 20 h, respectively) than deglycosylated A277 N and T236 N (57.2 and 45.1% of initial activity was remained, respectively).
31731975	5	14	theme	deglycosylated	1066:1079	arg1	T236 N					1088:1093	deglycosylated mutant T236 N	1066:1093	deglycosylated mutant T236 N (107, 91.9, and 42.8 h, respectively)	1066:1131	Furthermore, the glycosylated mutant T236 N showed longer half-lives in the presence of 25% (v/v) ethylene glycol, DMSO, and DMF (161, 133, and 56.7 h at 45 °C, respectively) than deglycosylated mutant T236 N (107, 91.9, and 42.8 h, respectively).
31731975	0	15	link	N-linked	0:7	arg1	glycosylation					9:21	N-linked glycosylation	0:21	N-linked glycosylation of thermostable lipase from Bacillus thermocatenulatus	0:76	N-linked glycosylation of thermostable lipase from Bacillus thermocatenulatus to improve organic solvent stability.
31731975	5	16	theme	DMSO	1001:1004	arg1	presence					962:969	the presence	958:969	the presence of 25% (v/v) ethylene glycol, DMSO, and DMF	958:1013	Furthermore, the glycosylated mutant T236 N showed longer half-lives in the presence of 25% (v/v) ethylene glycol, DMSO, and DMF (161, 133, and 56.7 h at 45 °C, respectively) than deglycosylated mutant T236 N (107, 91.9, and 42.8 h, respectively).
31731975	4	17	theme	glycosylated	760:771	arg1	mutants					773:779	These glycosylated mutants	754:779	These glycosylated mutants	754:779	These glycosylated mutants also showed higher remaining activity than wild-type BTL2 (56.0% of the initial activity were remained).
31731975	2	18	theme	BTL2	304:307	arg1	sequence					341:348	the consensus sequence	327:348	the consensus sequence	327:348	Among the eight BTL2 mutants including the consensus sequence, six BTL2 mutants, A277 N, A290 N, Y200 N, T236 N, T238 N, and P261 N, were glycosylated.
31731975	2	18	theme	BTL2	304:307	arg1	mutants					309:315	the eight BTL2 mutants	294:315	the eight BTL2 mutants including the consensus sequence	294:348	Among the eight BTL2 mutants including the consensus sequence, six BTL2 mutants, A277 N, A290 N, Y200 N, T236 N, T238 N, and P261 N, were glycosylated.
31731975	1	19	theme	consensus	202:210	arg1	sequence					212:219	the consensus sequence	198:219	the consensus sequence (-NXS/T-) for N-linked glycosylation	198:256	A thermostable lipase from Bacillus thermocatenulatus was glycosylated by forming the consensus sequence (-NXS/T-) for N-linked glycosylation by site-directed mutagenesis.
31731975	1	19	theme	consensus	202:210	arg1	-NXS/T-					222:228	-NXS/T-	222:228	-NXS/T-	222:228	A thermostable lipase from Bacillus thermocatenulatus was glycosylated by forming the consensus sequence (-NXS/T-) for N-linked glycosylation by site-directed mutagenesis.
31731975	1	20	from	thermocatenulatus	152:168	arg1	lipase					131:136	A thermostable lipase	116:136	A thermostable lipase from Bacillus thermocatenulatus	116:168	A thermostable lipase from Bacillus thermocatenulatus was glycosylated by forming the consensus sequence (-NXS/T-) for N-linked glycosylation by site-directed mutagenesis.
31731975	0	21	theme	N-linked	0:7	arg1	glycosylation					9:21	N-linked glycosylation	0:21	N-linked glycosylation of thermostable lipase from Bacillus thermocatenulatus	0:76	N-linked glycosylation of thermostable lipase from Bacillus thermocatenulatus to improve organic solvent stability.
31731975	3	22	theme	57.2	690:693	arg1	%					703:703	57.2 and 45.1%	690:703	%	703:703	Among the six mutants, glycosylated A277 N and T236 N showed higher stability in the presence of 25% (v/v) DMSO (74.3 and 72.8% of initial activity was remained after incubation at 45 °C for 20 h, respectively) than deglycosylated A277 N and T236 N (57.2 and 45.1% of initial activity was remained, respectively).
31731975	3	22	theme	57.2	690:693	arg1	activity					716:723	initial activity	708:723	initial activity	708:723	Among the six mutants, glycosylated A277 N and T236 N showed higher stability in the presence of 25% (v/v) DMSO (74.3 and 72.8% of initial activity was remained after incubation at 45 °C for 20 h, respectively) than deglycosylated A277 N and T236 N (57.2 and 45.1% of initial activity was remained, respectively).
31731975	5	23	gly	deglycosylated	1066:1079	arg1	T236 N					1088:1093	deglycosylated mutant T236 N	1066:1093	deglycosylated mutant T236 N (107, 91.9, and 42.8 h, respectively)	1066:1131	Furthermore, the glycosylated mutant T236 N showed longer half-lives in the presence of 25% (v/v) ethylene glycol, DMSO, and DMF (161, 133, and 56.7 h at 45 °C, respectively) than deglycosylated mutant T236 N (107, 91.9, and 42.8 h, respectively).
31731975	4	24	theme	higher	793:798	arg1	activity					810:817	higher remaining activity	793:817	higher remaining activity than wild-type BTL2	793:837	These glycosylated mutants also showed higher remaining activity than wild-type BTL2 (56.0% of the initial activity were remained).
31731975	0	25	theme	thermostable	26:37	arg1	lipase					39:44	thermostable lipase	26:44	thermostable lipase from Bacillus thermocatenulatus	26:76	N-linked glycosylation of thermostable lipase from Bacillus thermocatenulatus to improve organic solvent stability.
31731975	3	26	theme	activity	716:723	arg1	%					703:703	57.2 and 45.1%	690:703	%	703:703	Among the six mutants, glycosylated A277 N and T236 N showed higher stability in the presence of 25% (v/v) DMSO (74.3 and 72.8% of initial activity was remained after incubation at 45 °C for 20 h, respectively) than deglycosylated A277 N and T236 N (57.2 and 45.1% of initial activity was remained, respectively).
31731975	3	26	theme	activity	716:723	arg1	activity					716:723	initial activity	708:723	initial activity	708:723	Among the six mutants, glycosylated A277 N and T236 N showed higher stability in the presence of 25% (v/v) DMSO (74.3 and 72.8% of initial activity was remained after incubation at 45 °C for 20 h, respectively) than deglycosylated A277 N and T236 N (57.2 and 45.1% of initial activity was remained, respectively).
31731975	5	27	dep	T236 N	1088:1093	arg1	91.9					1101:1104	91.9	1101:1104	91.9	1101:1104	Furthermore, the glycosylated mutant T236 N showed longer half-lives in the presence of 25% (v/v) ethylene glycol, DMSO, and DMF (161, 133, and 56.7 h at 45 °C, respectively) than deglycosylated mutant T236 N (107, 91.9, and 42.8 h, respectively).
31731975	5	27	dep	T236 N	1088:1093	arg1	42.8 h					1111:1116	42.8 h	1111:1116	42.8 h	1111:1116	Furthermore, the glycosylated mutant T236 N showed longer half-lives in the presence of 25% (v/v) ethylene glycol, DMSO, and DMF (161, 133, and 56.7 h at 45 °C, respectively) than deglycosylated mutant T236 N (107, 91.9, and 42.8 h, respectively).
31731975	5	27	dep	T236 N	1088:1093	arg1	107					1096:1098	107	1096:1098	107	1096:1098	Furthermore, the glycosylated mutant T236 N showed longer half-lives in the presence of 25% (v/v) ethylene glycol, DMSO, and DMF (161, 133, and 56.7 h at 45 °C, respectively) than deglycosylated mutant T236 N (107, 91.9, and 42.8 h, respectively).
31731975	3	28	theme	%	539:539	arg1	DMSO					547:550	25% (v/v) DMSO	537:550	25% (v/v) DMSO	537:550	Among the six mutants, glycosylated A277 N and T236 N showed higher stability in the presence of 25% (v/v) DMSO (74.3 and 72.8% of initial activity was remained after incubation at 45 °C for 20 h, respectively) than deglycosylated A277 N and T236 N (57.2 and 45.1% of initial activity was remained, respectively).
31731975	5	29	theme	longer	937:942	arg1	half-lives					944:953	longer half-lives	937:953	longer half-lives	937:953	Furthermore, the glycosylated mutant T236 N showed longer half-lives in the presence of 25% (v/v) ethylene glycol, DMSO, and DMF (161, 133, and 56.7 h at 45 °C, respectively) than deglycosylated mutant T236 N (107, 91.9, and 42.8 h, respectively).
31731975	4	30	gly	glycosylated	760:771	arg1	mutants					773:779	These glycosylated mutants	754:779	These glycosylated mutants	754:779	These glycosylated mutants also showed higher remaining activity than wild-type BTL2 (56.0% of the initial activity were remained).
31731975	6	31	theme	solvents	1259:1266	arg1	presence					1239:1246	the presence	1235:1246	the presence of organic solvents	1235:1266	N-linked glycosylation may be a promising approach for preparing enzymes to retain their activity in the presence of organic solvents.
31731975	5	32	theme	DMF	1011:1013	arg1	presence					962:969	the presence	958:969	the presence of 25% (v/v) ethylene glycol, DMSO, and DMF	958:1013	Furthermore, the glycosylated mutant T236 N showed longer half-lives in the presence of 25% (v/v) ethylene glycol, DMSO, and DMF (161, 133, and 56.7 h at 45 °C, respectively) than deglycosylated mutant T236 N (107, 91.9, and 42.8 h, respectively).
31731975	6	33	theme	promising	1166:1174	arg1	glycosylation					1143:1155	N-linked glycosylation	1134:1155	N-linked glycosylation	1134:1155	N-linked glycosylation may be a promising approach for preparing enzymes to retain their activity in the presence of organic solvents.
31731975	6	33	theme	promising	1166:1174	arg1	approach					1176:1183	a promising approach	1164:1183	a promising approach for preparing enzymes to retain their activity in the presence of organic solvents	1164:1266	N-linked glycosylation may be a promising approach for preparing enzymes to retain their activity in the presence of organic solvents.
31731975	2	34	theme	consensus	331:339	arg1	sequence					341:348	the consensus sequence	327:348	the consensus sequence	327:348	Among the eight BTL2 mutants including the consensus sequence, six BTL2 mutants, A277 N, A290 N, Y200 N, T236 N, T238 N, and P261 N, were glycosylated.
31731975	5	35	dep	showed	930:935	arg1	45 °C					1040:1044	45 °C	1040:1044	45 °C	1040:1044	Furthermore, the glycosylated mutant T236 N showed longer half-lives in the presence of 25% (v/v) ethylene glycol, DMSO, and DMF (161, 133, and 56.7 h at 45 °C, respectively) than deglycosylated mutant T236 N (107, 91.9, and 42.8 h, respectively).
31731975	6	36	theme	organic	1251:1257	arg1	solvents					1259:1266	organic solvents	1251:1266	organic solvents	1251:1266	N-linked glycosylation may be a promising approach for preparing enzymes to retain their activity in the presence of organic solvents.
31731975	4	37	theme	initial	853:859	arg1	activity					861:868	the initial activity	849:868	the initial activity	849:868	These glycosylated mutants also showed higher remaining activity than wild-type BTL2 (56.0% of the initial activity were remained).
31731975	3	38	theme	74.3	553:556	arg1	%					566:566	74.3 and 72.8%	553:566	%	566:566	Among the six mutants, glycosylated A277 N and T236 N showed higher stability in the presence of 25% (v/v) DMSO (74.3 and 72.8% of initial activity was remained after incubation at 45 °C for 20 h, respectively) than deglycosylated A277 N and T236 N (57.2 and 45.1% of initial activity was remained, respectively).
31731975	3	38	theme	74.3	553:556	arg1	activity					579:586	initial activity	571:586	initial activity	571:586	Among the six mutants, glycosylated A277 N and T236 N showed higher stability in the presence of 25% (v/v) DMSO (74.3 and 72.8% of initial activity was remained after incubation at 45 °C for 20 h, respectively) than deglycosylated A277 N and T236 N (57.2 and 45.1% of initial activity was remained, respectively).
31731975	0	39	theme	lipase	39:44	arg1	glycosylation					9:21	N-linked glycosylation	0:21	N-linked glycosylation of thermostable lipase from Bacillus thermocatenulatus	0:76	N-linked glycosylation of thermostable lipase from Bacillus thermocatenulatus to improve organic solvent stability.
31731975	5	40	gly	glycosylated	903:914	arg1	T236 N					923:928	the glycosylated mutant T236 N	899:928	the glycosylated mutant T236 N	899:928	Furthermore, the glycosylated mutant T236 N showed longer half-lives in the presence of 25% (v/v) ethylene glycol, DMSO, and DMF (161, 133, and 56.7 h at 45 °C, respectively) than deglycosylated mutant T236 N (107, 91.9, and 42.8 h, respectively).
31731975	3	41	theme	activity	579:586	arg1	%					566:566	74.3 and 72.8%	553:566	%	566:566	Among the six mutants, glycosylated A277 N and T236 N showed higher stability in the presence of 25% (v/v) DMSO (74.3 and 72.8% of initial activity was remained after incubation at 45 °C for 20 h, respectively) than deglycosylated A277 N and T236 N (57.2 and 45.1% of initial activity was remained, respectively).
31731975	3	41	theme	activity	579:586	arg1	activity					579:586	initial activity	571:586	initial activity	571:586	Among the six mutants, glycosylated A277 N and T236 N showed higher stability in the presence of 25% (v/v) DMSO (74.3 and 72.8% of initial activity was remained after incubation at 45 °C for 20 h, respectively) than deglycosylated A277 N and T236 N (57.2 and 45.1% of initial activity was remained, respectively).
31731975	0	42	gly	glycosylation	9:21	arg1	lipase					39:44	thermostable lipase	26:44	thermostable lipase from Bacillus thermocatenulatus	26:76	N-linked glycosylation of thermostable lipase from Bacillus thermocatenulatus to improve organic solvent stability.
31731975	1	43	theme	N-linked	235:242	arg1	glycosylation					244:256	N-linked glycosylation	235:256	N-linked glycosylation	235:256	A thermostable lipase from Bacillus thermocatenulatus was glycosylated by forming the consensus sequence (-NXS/T-) for N-linked glycosylation by site-directed mutagenesis.
31731975	3	44	theme	initial	708:714	arg1	activity					716:723	initial activity	708:723	initial activity	708:723	Among the six mutants, glycosylated A277 N and T236 N showed higher stability in the presence of 25% (v/v) DMSO (74.3 and 72.8% of initial activity was remained after incubation at 45 °C for 20 h, respectively) than deglycosylated A277 N and T236 N (57.2 and 45.1% of initial activity was remained, respectively).
31731975	1	45	gly	glycosylated	174:185	arg1	lipase					131:136	A thermostable lipase	116:136	A thermostable lipase from Bacillus thermocatenulatus	116:168	A thermostable lipase from Bacillus thermocatenulatus was glycosylated by forming the consensus sequence (-NXS/T-) for N-linked glycosylation by site-directed mutagenesis.
31731975	5	46	theme	ethylene	984:991	arg1	glycol					993:998	25% (v/v) ethylene glycol	974:998	25% (v/v) ethylene glycol	974:998	Furthermore, the glycosylated mutant T236 N showed longer half-lives in the presence of 25% (v/v) ethylene glycol, DMSO, and DMF (161, 133, and 56.7 h at 45 °C, respectively) than deglycosylated mutant T236 N (107, 91.9, and 42.8 h, respectively).
31731975	3	47	theme	DMSO	547:550	arg1	presence					525:532	the presence	521:532	the presence of 25% (v/v) DMSO	521:550	Among the six mutants, glycosylated A277 N and T236 N showed higher stability in the presence of 25% (v/v) DMSO (74.3 and 72.8% of initial activity was remained after incubation at 45 °C for 20 h, respectively) than deglycosylated A277 N and T236 N (57.2 and 45.1% of initial activity was remained, respectively).
31731975	3	48	theme	45.1	699:702	arg1	%					703:703	57.2 and 45.1%	690:703	%	703:703	Among the six mutants, glycosylated A277 N and T236 N showed higher stability in the presence of 25% (v/v) DMSO (74.3 and 72.8% of initial activity was remained after incubation at 45 °C for 20 h, respectively) than deglycosylated A277 N and T236 N (57.2 and 45.1% of initial activity was remained, respectively).
31731975	3	48	theme	45.1	699:702	arg1	activity					716:723	initial activity	708:723	initial activity	708:723	Among the six mutants, glycosylated A277 N and T236 N showed higher stability in the presence of 25% (v/v) DMSO (74.3 and 72.8% of initial activity was remained after incubation at 45 °C for 20 h, respectively) than deglycosylated A277 N and T236 N (57.2 and 45.1% of initial activity was remained, respectively).
31731975	4	49	theme	wild-type	824:832	arg1	BTL2					834:837	wild-type BTL2	824:837	wild-type BTL2	824:837	These glycosylated mutants also showed higher remaining activity than wild-type BTL2 (56.0% of the initial activity were remained).
31731975	6	50	theme	N-linked	1134:1141	arg1	glycosylation					1143:1155	N-linked glycosylation	1134:1155	N-linked glycosylation	1134:1155	N-linked glycosylation may be a promising approach for preparing enzymes to retain their activity in the presence of organic solvents.
31731975	6	50	theme	N-linked	1134:1141	arg1	approach					1176:1183	a promising approach	1164:1183	a promising approach for preparing enzymes to retain their activity in the presence of organic solvents	1164:1266	N-linked glycosylation may be a promising approach for preparing enzymes to retain their activity in the presence of organic solvents.
31731975	5	51	theme	glycosylated	903:914	arg1	T236 N					923:928	the glycosylated mutant T236 N	899:928	the glycosylated mutant T236 N	899:928	Furthermore, the glycosylated mutant T236 N showed longer half-lives in the presence of 25% (v/v) ethylene glycol, DMSO, and DMF (161, 133, and 56.7 h at 45 °C, respectively) than deglycosylated mutant T236 N (107, 91.9, and 42.8 h, respectively).
31731975	5	52	theme	mutant	1081:1086	arg1	T236 N					1088:1093	deglycosylated mutant T236 N	1066:1093	deglycosylated mutant T236 N (107, 91.9, and 42.8 h, respectively)	1066:1131	Furthermore, the glycosylated mutant T236 N showed longer half-lives in the presence of 25% (v/v) ethylene glycol, DMSO, and DMF (161, 133, and 56.7 h at 45 °C, respectively) than deglycosylated mutant T236 N (107, 91.9, and 42.8 h, respectively).
31731975	5	53	theme	25	974:975	arg1	%					976:976	%	976:976	%	976:976	Furthermore, the glycosylated mutant T236 N showed longer half-lives in the presence of 25% (v/v) ethylene glycol, DMSO, and DMF (161, 133, and 56.7 h at 45 °C, respectively) than deglycosylated mutant T236 N (107, 91.9, and 42.8 h, respectively).
31731975	3	54	theme	initial	571:577	arg1	activity					579:586	initial activity	571:586	initial activity	571:586	Among the six mutants, glycosylated A277 N and T236 N showed higher stability in the presence of 25% (v/v) DMSO (74.3 and 72.8% of initial activity was remained after incubation at 45 °C for 20 h, respectively) than deglycosylated A277 N and T236 N (57.2 and 45.1% of initial activity was remained, respectively).
31731975	2	55	theme	BTL2	355:358	arg1	A290 N					377:382	A290 N	377:382	A290 N	377:382	Among the eight BTL2 mutants including the consensus sequence, six BTL2 mutants, A277 N, A290 N, Y200 N, T236 N, T238 N, and P261 N, were glycosylated.
31731975	2	55	theme	BTL2	355:358	arg1	T238 N					401:406	T238 N	401:406	T238 N	401:406	Among the eight BTL2 mutants including the consensus sequence, six BTL2 mutants, A277 N, A290 N, Y200 N, T236 N, T238 N, and P261 N, were glycosylated.
31731975	2	55	theme	BTL2	355:358	arg1	P261 N					413:418	P261 N	413:418	P261 N	413:418	Among the eight BTL2 mutants including the consensus sequence, six BTL2 mutants, A277 N, A290 N, Y200 N, T236 N, T238 N, and P261 N, were glycosylated.
31731975	2	55	theme	BTL2	355:358	arg1	mutants					360:366	six BTL2 mutants	351:366	six BTL2 mutants	351:366	Among the eight BTL2 mutants including the consensus sequence, six BTL2 mutants, A277 N, A290 N, Y200 N, T236 N, T238 N, and P261 N, were glycosylated.
31731975	2	55	theme	BTL2	355:358	arg1	T236 N					393:398	T236 N	393:398	T236 N	393:398	Among the eight BTL2 mutants including the consensus sequence, six BTL2 mutants, A277 N, A290 N, Y200 N, T236 N, T238 N, and P261 N, were glycosylated.
31731975	5	56	theme	%	976:976	arg1	glycol					993:998	25% (v/v) ethylene glycol	974:998	25% (v/v) ethylene glycol	974:998	Furthermore, the glycosylated mutant T236 N showed longer half-lives in the presence of 25% (v/v) ethylene glycol, DMSO, and DMF (161, 133, and 56.7 h at 45 °C, respectively) than deglycosylated mutant T236 N (107, 91.9, and 42.8 h, respectively).
31731975	3	57	theme	72.8	562:565	arg1	%					566:566	74.3 and 72.8%	553:566	%	566:566	Among the six mutants, glycosylated A277 N and T236 N showed higher stability in the presence of 25% (v/v) DMSO (74.3 and 72.8% of initial activity was remained after incubation at 45 °C for 20 h, respectively) than deglycosylated A277 N and T236 N (57.2 and 45.1% of initial activity was remained, respectively).
31731975	3	57	theme	72.8	562:565	arg1	activity					579:586	initial activity	571:586	initial activity	571:586	Among the six mutants, glycosylated A277 N and T236 N showed higher stability in the presence of 25% (v/v) DMSO (74.3 and 72.8% of initial activity was remained after incubation at 45 °C for 20 h, respectively) than deglycosylated A277 N and T236 N (57.2 and 45.1% of initial activity was remained, respectively).
31731975	3	58	dep	%	539:539	arg1	v/v					542:544	v/v	542:544	v/v	542:544	Among the six mutants, glycosylated A277 N and T236 N showed higher stability in the presence of 25% (v/v) DMSO (74.3 and 72.8% of initial activity was remained after incubation at 45 °C for 20 h, respectively) than deglycosylated A277 N and T236 N (57.2 and 45.1% of initial activity was remained, respectively).
31731975	1	59	theme	site-directed	261:273	arg1	mutagenesis					275:285	site-directed mutagenesis	261:285	site-directed mutagenesis	261:285	A thermostable lipase from Bacillus thermocatenulatus was glycosylated by forming the consensus sequence (-NXS/T-) for N-linked glycosylation by site-directed mutagenesis.
31731975	5	60	dep	45 °C	1040:1044	arg1	133					1021:1023	133	1021:1023	133	1021:1023	Furthermore, the glycosylated mutant T236 N showed longer half-lives in the presence of 25% (v/v) ethylene glycol, DMSO, and DMF (161, 133, and 56.7 h at 45 °C, respectively) than deglycosylated mutant T236 N (107, 91.9, and 42.8 h, respectively).
31731975	5	60	dep	45 °C	1040:1044	arg1	56.7 h					1030:1035	56.7 h	1030:1035	56.7 h	1030:1035	Furthermore, the glycosylated mutant T236 N showed longer half-lives in the presence of 25% (v/v) ethylene glycol, DMSO, and DMF (161, 133, and 56.7 h at 45 °C, respectively) than deglycosylated mutant T236 N (107, 91.9, and 42.8 h, respectively).
31731975	2	61	gly	glycosylated	426:437	arg1	A290 N					377:382	A290 N	377:382	A290 N	377:382	Among the eight BTL2 mutants including the consensus sequence, six BTL2 mutants, A277 N, A290 N, Y200 N, T236 N, T238 N, and P261 N, were glycosylated.
31731975	2	61	gly	glycosylated	426:437	arg1	T238 N					401:406	T238 N	401:406	T238 N	401:406	Among the eight BTL2 mutants including the consensus sequence, six BTL2 mutants, A277 N, A290 N, Y200 N, T236 N, T238 N, and P261 N, were glycosylated.
31731975	2	61	gly	glycosylated	426:437	arg1	P261 N					413:418	P261 N	413:418	P261 N	413:418	Among the eight BTL2 mutants including the consensus sequence, six BTL2 mutants, A277 N, A290 N, Y200 N, T236 N, T238 N, and P261 N, were glycosylated.
31731975	2	61	gly	glycosylated	426:437	arg1	mutants					360:366	six BTL2 mutants	351:366	six BTL2 mutants	351:366	Among the eight BTL2 mutants including the consensus sequence, six BTL2 mutants, A277 N, A290 N, Y200 N, T236 N, T238 N, and P261 N, were glycosylated.
31731975	2	61	gly	glycosylated	426:437	arg1	T236 N					393:398	T236 N	393:398	T236 N	393:398	Among the eight BTL2 mutants including the consensus sequence, six BTL2 mutants, A277 N, A290 N, Y200 N, T236 N, T238 N, and P261 N, were glycosylated.
31731975	6	62	link	N-linked	1134:1141	arg1	glycosylation					1143:1155	N-linked glycosylation	1134:1155	N-linked glycosylation	1134:1155	N-linked glycosylation may be a promising approach for preparing enzymes to retain their activity in the presence of organic solvents.
31731975	6	62	link	N-linked	1134:1141	arg1	approach					1176:1183	a promising approach	1164:1183	a promising approach for preparing enzymes to retain their activity in the presence of organic solvents	1164:1266	N-linked glycosylation may be a promising approach for preparing enzymes to retain their activity in the presence of organic solvents.
31390701	2	0	theme	13C	583:585	arg1	angle					612:616	solid-state 13C cross-polarization magic angle spinning (CP-MAS) and solution-state 1H,13C heteronuclear single-quantum coherence (HSQC) nuclear magnetic resonance (NMR) spectroscopy	571:752	solid-state 13C cross-polarization magic angle spinning (CP-MAS) and solution-state 1H,13C heteronuclear single-quantum coherence (HSQC) nuclear magnetic resonance (NMR) spectroscopy	571:752	Molecular properties of POM in influent (substrate) and effluent (digestate) of seven full-scale AD plants (three agricultural waste and four food waste digesters) were characterized and compared using solid-state 13C cross-polarization magic angle spinning (CP-MAS) and solution-state 1H,13C heteronuclear single-quantum coherence (HSQC) nuclear magnetic resonance (NMR) spectroscopy.
31390701	5	1	theme	POM	1439:1441	arg1	structures					1443:1452	recalcitrant POM structures	1426:1452	recalcitrant POM structures	1426:1452	Accordingly, the labile POM fraction in food and agricultural solid wastes is characterized by structural entities of lipids and starch-like carbohydrates, whereas recalcitrant POM structures resemble alkyl and aromatic subunits of amino acids, lignin, and polysaccharides with β-glycosidic linkages.
31390701	1	2	theme	molecular	265:273	arg1	properties					275:284	the molecular properties	261:284	the molecular properties of the recalcitrant POM fraction, which remains in the digestate after AD process	261:366	This study assesses the molecular characteristics of particulate organic matter (POM) in agricultural and food waste digesters and elucidates the molecular properties of the recalcitrant POM fraction, which remains in the digestate after AD process.
31390701	2	3	theme	spinning	618:625	arg1	CP-MAS					628:633	spinning (CP-MAS) and solution-state 1H,13C heteronuclear single-quantum coherence (HSQC) nuclear magnetic resonance (NMR) spectroscopy	618:752	CP-MAS	628:633	Molecular properties of POM in influent (substrate) and effluent (digestate) of seven full-scale AD plants (three agricultural waste and four food waste digesters) were characterized and compared using solid-state 13C cross-polarization magic angle spinning (CP-MAS) and solution-state 1H,13C heteronuclear single-quantum coherence (HSQC) nuclear magnetic resonance (NMR) spectroscopy.
31390701	4	4	theme	recalcitrant	1133:1144	arg1	fractions					1146:1154	labile and recalcitrant fractions	1122:1154	labile and recalcitrant fractions of POM	1122:1161	Distinctive molecular structures of labile and recalcitrant fractions of POM, subjected to AD, were identified by comparing the NMR spectra of all substrate and digestate POM.
31390701	5	5	theme	carbohydrates	1403:1415	arg1	entities					1368:1375	structural entities	1357:1375	structural entities of lipids and starch-like carbohydrates	1357:1415	Accordingly, the labile POM fraction in food and agricultural solid wastes is characterized by structural entities of lipids and starch-like carbohydrates, whereas recalcitrant POM structures resemble alkyl and aromatic subunits of amino acids, lignin, and polysaccharides with β-glycosidic linkages.
31390701	1	6	theme	organic	184:190	arg1	matter					192:197	particulate organic matter	172:197	particulate organic matter (POM)	172:203	This study assesses the molecular characteristics of particulate organic matter (POM) in agricultural and food waste digesters and elucidates the molecular properties of the recalcitrant POM fraction, which remains in the digestate after AD process.
31390701	1	6	theme	organic	184:190	arg1	POM					200:202	POM	200:202	POM	200:202	This study assesses the molecular characteristics of particulate organic matter (POM) in agricultural and food waste digesters and elucidates the molecular properties of the recalcitrant POM fraction, which remains in the digestate after AD process.
31390701	2	7	theme	magic	606:610	arg1	angle					612:616	solid-state 13C cross-polarization magic angle spinning (CP-MAS) and solution-state 1H,13C heteronuclear single-quantum coherence (HSQC) nuclear magnetic resonance (NMR) spectroscopy	571:752	solid-state 13C cross-polarization magic angle spinning (CP-MAS) and solution-state 1H,13C heteronuclear single-quantum coherence (HSQC) nuclear magnetic resonance (NMR) spectroscopy	571:752	Molecular properties of POM in influent (substrate) and effluent (digestate) of seven full-scale AD plants (three agricultural waste and four food waste digesters) were characterized and compared using solid-state 13C cross-polarization magic angle spinning (CP-MAS) and solution-state 1H,13C heteronuclear single-quantum coherence (HSQC) nuclear magnetic resonance (NMR) spectroscopy.
31390701	2	8	theme	NMR	736:738	arg1	spectroscopy					741:752	spinning (CP-MAS) and solution-state 1H,13C heteronuclear single-quantum coherence (HSQC) nuclear magnetic resonance (NMR) spectroscopy	618:752	spectroscopy	741:752	Molecular properties of POM in influent (substrate) and effluent (digestate) of seven full-scale AD plants (three agricultural waste and four food waste digesters) were characterized and compared using solid-state 13C cross-polarization magic angle spinning (CP-MAS) and solution-state 1H,13C heteronuclear single-quantum coherence (HSQC) nuclear magnetic resonance (NMR) spectroscopy.
31390701	3	9	theme	biomass	1037:1043	arg1	increase					1015:1022	increase	1015:1022	increase of microbial biomass upon AD of agricultural and food wastes	1015:1083	Comparison of the POM structural compositions of substrate and digestate from each AD plant revealed an enrichment of protein structures relative to the carbohydrates in most cases, implying a preferential degradation of the carbohydrates over proteins and/or increase of microbial biomass upon AD of agricultural and food wastes.
31390701	2	10	theme	food	511:514	arg1	digesters					522:530	four food waste digesters	506:530	four food waste digesters	506:530	Molecular properties of POM in influent (substrate) and effluent (digestate) of seven full-scale AD plants (three agricultural waste and four food waste digesters) were characterized and compared using solid-state 13C cross-polarization magic angle spinning (CP-MAS) and solution-state 1H,13C heteronuclear single-quantum coherence (HSQC) nuclear magnetic resonance (NMR) spectroscopy.
31390701	6	11	theme	matter	1707:1712	arg1	fate					1691:1694	the fate	1687:1694	the fate of organic matter in digestate-amended arable lands	1687:1746	This information serves as a basis to further explore optimization approaches for improving AD of the underutilized POM and the fate of organic matter in digestate-amended arable lands.
31390701	6	11	theme	matter	1707:1712	arg1	AD					1655:1656	AD	1655:1656	AD of the underutilized POM	1655:1681	This information serves as a basis to further explore optimization approaches for improving AD of the underutilized POM and the fate of organic matter in digestate-amended arable lands.
31390701	4	12	theme	labile	1122:1127	arg1	fractions					1146:1154	labile and recalcitrant fractions	1122:1154	labile and recalcitrant fractions of POM	1122:1161	Distinctive molecular structures of labile and recalcitrant fractions of POM, subjected to AD, were identified by comparing the NMR spectra of all substrate and digestate POM.
31390701	1	13	theme	agricultural	208:219	arg1	digesters					236:244	agricultural and food waste digesters	208:244	agricultural and food waste digesters	208:244	This study assesses the molecular characteristics of particulate organic matter (POM) in agricultural and food waste digesters and elucidates the molecular properties of the recalcitrant POM fraction, which remains in the digestate after AD process.
31390701	5	14	from	fraction	1290:1297	arg1	food					1302:1305	food	1302:1305	food	1302:1305	Accordingly, the labile POM fraction in food and agricultural solid wastes is characterized by structural entities of lipids and starch-like carbohydrates, whereas recalcitrant POM structures resemble alkyl and aromatic subunits of amino acids, lignin, and polysaccharides with β-glycosidic linkages.
31390701	5	14	from	fraction	1290:1297	arg1	wastes					1330:1335	agricultural solid wastes	1311:1335	agricultural solid wastes	1311:1335	Accordingly, the labile POM fraction in food and agricultural solid wastes is characterized by structural entities of lipids and starch-like carbohydrates, whereas recalcitrant POM structures resemble alkyl and aromatic subunits of amino acids, lignin, and polysaccharides with β-glycosidic linkages.
31390701	2	15	theme	HSQC	702:705	arg1	spectroscopy					741:752	spinning (CP-MAS) and solution-state 1H,13C heteronuclear single-quantum coherence (HSQC) nuclear magnetic resonance (NMR) spectroscopy	618:752	spectroscopy	741:752	Molecular properties of POM in influent (substrate) and effluent (digestate) of seven full-scale AD plants (three agricultural waste and four food waste digesters) were characterized and compared using solid-state 13C cross-polarization magic angle spinning (CP-MAS) and solution-state 1H,13C heteronuclear single-quantum coherence (HSQC) nuclear magnetic resonance (NMR) spectroscopy.
31390701	2	16	theme	solution-state	640:653	arg1	spectroscopy					741:752	spinning (CP-MAS) and solution-state 1H,13C heteronuclear single-quantum coherence (HSQC) nuclear magnetic resonance (NMR) spectroscopy	618:752	spectroscopy	741:752	Molecular properties of POM in influent (substrate) and effluent (digestate) of seven full-scale AD plants (three agricultural waste and four food waste digesters) were characterized and compared using solid-state 13C cross-polarization magic angle spinning (CP-MAS) and solution-state 1H,13C heteronuclear single-quantum coherence (HSQC) nuclear magnetic resonance (NMR) spectroscopy.
31390701	2	17	theme	coherence	691:699	arg1	spectroscopy					741:752	spinning (CP-MAS) and solution-state 1H,13C heteronuclear single-quantum coherence (HSQC) nuclear magnetic resonance (NMR) spectroscopy	618:752	spectroscopy	741:752	Molecular properties of POM in influent (substrate) and effluent (digestate) of seven full-scale AD plants (three agricultural waste and four food waste digesters) were characterized and compared using solid-state 13C cross-polarization magic angle spinning (CP-MAS) and solution-state 1H,13C heteronuclear single-quantum coherence (HSQC) nuclear magnetic resonance (NMR) spectroscopy.
31390701	3	18	theme	AD	838:839	arg1	plant					841:845	each AD plant	833:845	each AD plant	833:845	Comparison of the POM structural compositions of substrate and digestate from each AD plant revealed an enrichment of protein structures relative to the carbohydrates in most cases, implying a preferential degradation of the carbohydrates over proteins and/or increase of microbial biomass upon AD of agricultural and food wastes.
31390701	6	19	theme	POM	1679:1681	arg1	fate					1691:1694	the fate	1687:1694	the fate of organic matter in digestate-amended arable lands	1687:1746	This information serves as a basis to further explore optimization approaches for improving AD of the underutilized POM and the fate of organic matter in digestate-amended arable lands.
31390701	6	19	theme	POM	1679:1681	arg1	AD					1655:1656	AD	1655:1656	AD of the underutilized POM	1655:1681	This information serves as a basis to further explore optimization approaches for improving AD of the underutilized POM and the fate of organic matter in digestate-amended arable lands.
31390701	2	20	theme	resonance	725:733	arg1	spectroscopy					741:752	spinning (CP-MAS) and solution-state 1H,13C heteronuclear single-quantum coherence (HSQC) nuclear magnetic resonance (NMR) spectroscopy	618:752	spectroscopy	741:752	Molecular properties of POM in influent (substrate) and effluent (digestate) of seven full-scale AD plants (three agricultural waste and four food waste digesters) were characterized and compared using solid-state 13C cross-polarization magic angle spinning (CP-MAS) and solution-state 1H,13C heteronuclear single-quantum coherence (HSQC) nuclear magnetic resonance (NMR) spectroscopy.
31390701	5	21	theme	POM	1286:1288	arg1	fraction					1290:1297	the labile POM fraction	1275:1297	the labile POM fraction in food and agricultural solid wastes	1275:1335	Accordingly, the labile POM fraction in food and agricultural solid wastes is characterized by structural entities of lipids and starch-like carbohydrates, whereas recalcitrant POM structures resemble alkyl and aromatic subunits of amino acids, lignin, and polysaccharides with β-glycosidic linkages.
31390701	3	22	theme	structural	777:786	arg1	compositions					788:799	the POM structural compositions	769:799	the POM structural compositions of substrate and digestate from each AD plant	769:845	Comparison of the POM structural compositions of substrate and digestate from each AD plant revealed an enrichment of protein structures relative to the carbohydrates in most cases, implying a preferential degradation of the carbohydrates over proteins and/or increase of microbial biomass upon AD of agricultural and food wastes.
31390701	0	23	theme	scale	65:69	arg1	digesters					81:89	full scale anaerobic digesters	60:89	full scale anaerobic digesters	60:89	Molecular characterization of particulate organic matter in full scale anaerobic digesters: An NMR spectroscopy study.
31390701	1	24	theme	molecular	143:151	arg1	characteristics					153:167	the molecular characteristics	139:167	the molecular characteristics of particulate organic matter (POM)	139:203	This study assesses the molecular characteristics of particulate organic matter (POM) in agricultural and food waste digesters and elucidates the molecular properties of the recalcitrant POM fraction, which remains in the digestate after AD process.
31390701	3	25	theme	protein	873:879	arg1	structures					881:890	protein structures	873:890	protein structures relative to the carbohydrates in most cases	873:934	Comparison of the POM structural compositions of substrate and digestate from each AD plant revealed an enrichment of protein structures relative to the carbohydrates in most cases, implying a preferential degradation of the carbohydrates over proteins and/or increase of microbial biomass upon AD of agricultural and food wastes.
31390701	2	26	theme	AD	466:467	arg1	plants					469:474	seven full-scale AD plants	449:474	seven full-scale AD plants (three agricultural waste and four food waste digesters)	449:531	Molecular properties of POM in influent (substrate) and effluent (digestate) of seven full-scale AD plants (three agricultural waste and four food waste digesters) were characterized and compared using solid-state 13C cross-polarization magic angle spinning (CP-MAS) and solution-state 1H,13C heteronuclear single-quantum coherence (HSQC) nuclear magnetic resonance (NMR) spectroscopy.
31390701	3	27	from	Comparison	755:764	arg1	plant					841:845	each AD plant	833:845	each AD plant	833:845	Comparison of the POM structural compositions of substrate and digestate from each AD plant revealed an enrichment of protein structures relative to the carbohydrates in most cases, implying a preferential degradation of the carbohydrates over proteins and/or increase of microbial biomass upon AD of agricultural and food wastes.
31390701	2	28	theme	nuclear	708:714	arg1	spectroscopy					741:752	spinning (CP-MAS) and solution-state 1H,13C heteronuclear single-quantum coherence (HSQC) nuclear magnetic resonance (NMR) spectroscopy	618:752	spectroscopy	741:752	Molecular properties of POM in influent (substrate) and effluent (digestate) of seven full-scale AD plants (three agricultural waste and four food waste digesters) were characterized and compared using solid-state 13C cross-polarization magic angle spinning (CP-MAS) and solution-state 1H,13C heteronuclear single-quantum coherence (HSQC) nuclear magnetic resonance (NMR) spectroscopy.
31390701	3	29	theme	food	1073:1076	arg1	wastes					1078:1083	agricultural and food wastes	1056:1083	agricultural and food wastes	1056:1083	Comparison of the POM structural compositions of substrate and digestate from each AD plant revealed an enrichment of protein structures relative to the carbohydrates in most cases, implying a preferential degradation of the carbohydrates over proteins and/or increase of microbial biomass upon AD of agricultural and food wastes.
31390701	6	30	theme	underutilized	1665:1677	arg1	POM					1679:1681	the underutilized POM	1661:1681	the underutilized POM	1661:1681	This information serves as a basis to further explore optimization approaches for improving AD of the underutilized POM and the fate of organic matter in digestate-amended arable lands.
31390701	2	31	from	effluent	425:432	arg1	properties					379:388	Molecular properties	369:388	Molecular properties of POM in influent (substrate) and effluent (digestate) of seven full-scale AD plants (three agricultural waste and four food waste digesters)	369:531	Molecular properties of POM in influent (substrate) and effluent (digestate) of seven full-scale AD plants (three agricultural waste and four food waste digesters) were characterized and compared using solid-state 13C cross-polarization magic angle spinning (CP-MAS) and solution-state 1H,13C heteronuclear single-quantum coherence (HSQC) nuclear magnetic resonance (NMR) spectroscopy.
31390701	3	32	theme	agricultural	1056:1067	arg1	wastes					1078:1083	agricultural and food wastes	1056:1083	agricultural and food wastes	1056:1083	Comparison of the POM structural compositions of substrate and digestate from each AD plant revealed an enrichment of protein structures relative to the carbohydrates in most cases, implying a preferential degradation of the carbohydrates over proteins and/or increase of microbial biomass upon AD of agricultural and food wastes.
31390701	2	33	theme	agricultural	483:494	arg1	waste					496:500	three agricultural waste	477:500	three agricultural waste	477:500	Molecular properties of POM in influent (substrate) and effluent (digestate) of seven full-scale AD plants (three agricultural waste and four food waste digesters) were characterized and compared using solid-state 13C cross-polarization magic angle spinning (CP-MAS) and solution-state 1H,13C heteronuclear single-quantum coherence (HSQC) nuclear magnetic resonance (NMR) spectroscopy.
31390701	4	34	theme	substrate	1233:1241	arg1	POM					1257:1259	all substrate and digestate POM	1229:1259	POM	1257:1259	Distinctive molecular structures of labile and recalcitrant fractions of POM, subjected to AD, were identified by comparing the NMR spectra of all substrate and digestate POM.
31390701	5	35	theme	solid	1324:1328	arg1	wastes					1330:1335	agricultural solid wastes	1311:1335	agricultural solid wastes	1311:1335	Accordingly, the labile POM fraction in food and agricultural solid wastes is characterized by structural entities of lipids and starch-like carbohydrates, whereas recalcitrant POM structures resemble alkyl and aromatic subunits of amino acids, lignin, and polysaccharides with β-glycosidic linkages.
31390701	2	36	theme	POM	393:395	arg1	properties					379:388	Molecular properties	369:388	Molecular properties of POM in influent (substrate) and effluent (digestate) of seven full-scale AD plants (three agricultural waste and four food waste digesters)	369:531	Molecular properties of POM in influent (substrate) and effluent (digestate) of seven full-scale AD plants (three agricultural waste and four food waste digesters) were characterized and compared using solid-state 13C cross-polarization magic angle spinning (CP-MAS) and solution-state 1H,13C heteronuclear single-quantum coherence (HSQC) nuclear magnetic resonance (NMR) spectroscopy.
31390701	0	37	theme	spectroscopy	99:110	arg1	study					112:116	An NMR spectroscopy study	92:116	Molecular characterization of particulate organic matter in full scale anaerobic digesters: An NMR spectroscopy study.	0:117	Molecular characterization of particulate organic matter in full scale anaerobic digesters: An NMR spectroscopy study.
31390701	3	38	theme	relative	892:899	arg1	structures					881:890	protein structures	873:890	protein structures relative to the carbohydrates in most cases	873:934	Comparison of the POM structural compositions of substrate and digestate from each AD plant revealed an enrichment of protein structures relative to the carbohydrates in most cases, implying a preferential degradation of the carbohydrates over proteins and/or increase of microbial biomass upon AD of agricultural and food wastes.
31390701	6	39	theme	digestate-amended	1717:1733	arg1	lands					1742:1746	digestate-amended arable lands	1717:1746	digestate-amended arable lands	1717:1746	This information serves as a basis to further explore optimization approaches for improving AD of the underutilized POM and the fate of organic matter in digestate-amended arable lands.
31390701	2	40	theme	heteronuclear	662:674	arg1	spectroscopy					741:752	spinning (CP-MAS) and solution-state 1H,13C heteronuclear single-quantum coherence (HSQC) nuclear magnetic resonance (NMR) spectroscopy	618:752	spectroscopy	741:752	Molecular properties of POM in influent (substrate) and effluent (digestate) of seven full-scale AD plants (three agricultural waste and four food waste digesters) were characterized and compared using solid-state 13C cross-polarization magic angle spinning (CP-MAS) and solution-state 1H,13C heteronuclear single-quantum coherence (HSQC) nuclear magnetic resonance (NMR) spectroscopy.
31390701	0	41	theme	Molecular	0:8	arg1	characterization					10:25	Molecular characterization	0:25	Molecular characterization of particulate organic matter in full scale anaerobic digesters: An NMR spectroscopy study.	0:117	Molecular characterization of particulate organic matter in full scale anaerobic digesters: An NMR spectroscopy study.
31390701	5	42	theme	β-glycosidic	1540:1551	arg1	linkages					1553:1560	β-glycosidic linkages	1540:1560	β-glycosidic linkages	1540:1560	Accordingly, the labile POM fraction in food and agricultural solid wastes is characterized by structural entities of lipids and starch-like carbohydrates, whereas recalcitrant POM structures resemble alkyl and aromatic subunits of amino acids, lignin, and polysaccharides with β-glycosidic linkages.
31390701	4	43	theme	NMR	1214:1216	arg1	spectra					1218:1224	the NMR spectra	1210:1224	the NMR spectra of all substrate and digestate POM	1210:1259	Distinctive molecular structures of labile and recalcitrant fractions of POM, subjected to AD, were identified by comparing the NMR spectra of all substrate and digestate POM.
31390701	5	44	dep	characterized	1340:1352	arg1	whereas					1418:1424	whereas	1418:1424	whereas	1418:1424	Accordingly, the labile POM fraction in food and agricultural solid wastes is characterized by structural entities of lipids and starch-like carbohydrates, whereas recalcitrant POM structures resemble alkyl and aromatic subunits of amino acids, lignin, and polysaccharides with β-glycosidic linkages.
31390701	0	45	theme	organic	42:48	arg1	matter					50:55	particulate organic matter	30:55	particulate organic matter	30:55	Molecular characterization of particulate organic matter in full scale anaerobic digesters: An NMR spectroscopy study.
31390701	1	46	theme	POM	306:308	arg1	fraction					310:317	the recalcitrant POM fraction	289:317	the recalcitrant POM fraction	289:317	This study assesses the molecular characteristics of particulate organic matter (POM) in agricultural and food waste digesters and elucidates the molecular properties of the recalcitrant POM fraction, which remains in the digestate after AD process.
31390701	2	47	from	properties	379:388	arg1	effluent					425:432	effluent	425:432	effluent (digestate)	425:444	Molecular properties of POM in influent (substrate) and effluent (digestate) of seven full-scale AD plants (three agricultural waste and four food waste digesters) were characterized and compared using solid-state 13C cross-polarization magic angle spinning (CP-MAS) and solution-state 1H,13C heteronuclear single-quantum coherence (HSQC) nuclear magnetic resonance (NMR) spectroscopy.
31390701	2	47	from	properties	379:388	arg1	influent					400:407	influent	400:407	influent (substrate)	400:419	Molecular properties of POM in influent (substrate) and effluent (digestate) of seven full-scale AD plants (three agricultural waste and four food waste digesters) were characterized and compared using solid-state 13C cross-polarization magic angle spinning (CP-MAS) and solution-state 1H,13C heteronuclear single-quantum coherence (HSQC) nuclear magnetic resonance (NMR) spectroscopy.
31390701	2	47	from	properties	379:388	arg1	digestate					435:443	digestate	435:443	digestate	435:443	Molecular properties of POM in influent (substrate) and effluent (digestate) of seven full-scale AD plants (three agricultural waste and four food waste digesters) were characterized and compared using solid-state 13C cross-polarization magic angle spinning (CP-MAS) and solution-state 1H,13C heteronuclear single-quantum coherence (HSQC) nuclear magnetic resonance (NMR) spectroscopy.
31390701	2	47	from	properties	379:388	arg1	substrate					410:418	substrate	410:418	substrate	410:418	Molecular properties of POM in influent (substrate) and effluent (digestate) of seven full-scale AD plants (three agricultural waste and four food waste digesters) were characterized and compared using solid-state 13C cross-polarization magic angle spinning (CP-MAS) and solution-state 1H,13C heteronuclear single-quantum coherence (HSQC) nuclear magnetic resonance (NMR) spectroscopy.
31390701	6	48	from	fate	1691:1694	arg1	lands					1742:1746	digestate-amended arable lands	1717:1746	digestate-amended arable lands	1717:1746	This information serves as a basis to further explore optimization approaches for improving AD of the underutilized POM and the fate of organic matter in digestate-amended arable lands.
31390701	5	49	theme	lipids	1380:1385	arg1	entities					1368:1375	structural entities	1357:1375	structural entities of lipids and starch-like carbohydrates	1357:1415	Accordingly, the labile POM fraction in food and agricultural solid wastes is characterized by structural entities of lipids and starch-like carbohydrates, whereas recalcitrant POM structures resemble alkyl and aromatic subunits of amino acids, lignin, and polysaccharides with β-glycosidic linkages.
31390701	2	50	from	POM	393:395	arg1	effluent					425:432	effluent	425:432	effluent (digestate)	425:444	Molecular properties of POM in influent (substrate) and effluent (digestate) of seven full-scale AD plants (three agricultural waste and four food waste digesters) were characterized and compared using solid-state 13C cross-polarization magic angle spinning (CP-MAS) and solution-state 1H,13C heteronuclear single-quantum coherence (HSQC) nuclear magnetic resonance (NMR) spectroscopy.
31390701	2	50	from	POM	393:395	arg1	influent					400:407	influent	400:407	influent (substrate)	400:419	Molecular properties of POM in influent (substrate) and effluent (digestate) of seven full-scale AD plants (three agricultural waste and four food waste digesters) were characterized and compared using solid-state 13C cross-polarization magic angle spinning (CP-MAS) and solution-state 1H,13C heteronuclear single-quantum coherence (HSQC) nuclear magnetic resonance (NMR) spectroscopy.
31390701	2	50	from	POM	393:395	arg1	digestate					435:443	digestate	435:443	digestate	435:443	Molecular properties of POM in influent (substrate) and effluent (digestate) of seven full-scale AD plants (three agricultural waste and four food waste digesters) were characterized and compared using solid-state 13C cross-polarization magic angle spinning (CP-MAS) and solution-state 1H,13C heteronuclear single-quantum coherence (HSQC) nuclear magnetic resonance (NMR) spectroscopy.
31390701	2	50	from	POM	393:395	arg1	substrate					410:418	substrate	410:418	substrate	410:418	Molecular properties of POM in influent (substrate) and effluent (digestate) of seven full-scale AD plants (three agricultural waste and four food waste digesters) were characterized and compared using solid-state 13C cross-polarization magic angle spinning (CP-MAS) and solution-state 1H,13C heteronuclear single-quantum coherence (HSQC) nuclear magnetic resonance (NMR) spectroscopy.
31390701	5	51	theme	polysaccharides	1519:1533	arg1	subunits					1482:1489	alkyl and aromatic subunits	1463:1489	alkyl and aromatic subunits of amino acids, lignin, and polysaccharides with β-glycosidic linkages	1463:1560	Accordingly, the labile POM fraction in food and agricultural solid wastes is characterized by structural entities of lipids and starch-like carbohydrates, whereas recalcitrant POM structures resemble alkyl and aromatic subunits of amino acids, lignin, and polysaccharides with β-glycosidic linkages.
31390701	4	52	theme	digestate	1247:1255	arg1	POM					1257:1259	all substrate and digestate POM	1229:1259	POM	1257:1259	Distinctive molecular structures of labile and recalcitrant fractions of POM, subjected to AD, were identified by comparing the NMR spectra of all substrate and digestate POM.
31390701	5	53	theme	starch-like	1391:1401	arg1	carbohydrates					1403:1415	starch-like carbohydrates	1391:1415	starch-like carbohydrates	1391:1415	Accordingly, the labile POM fraction in food and agricultural solid wastes is characterized by structural entities of lipids and starch-like carbohydrates, whereas recalcitrant POM structures resemble alkyl and aromatic subunits of amino acids, lignin, and polysaccharides with β-glycosidic linkages.
31390701	2	54	dep	plants	469:474	arg1	digesters					522:530	four food waste digesters	506:530	four food waste digesters	506:530	Molecular properties of POM in influent (substrate) and effluent (digestate) of seven full-scale AD plants (three agricultural waste and four food waste digesters) were characterized and compared using solid-state 13C cross-polarization magic angle spinning (CP-MAS) and solution-state 1H,13C heteronuclear single-quantum coherence (HSQC) nuclear magnetic resonance (NMR) spectroscopy.
31390701	2	54	dep	plants	469:474	arg1	waste					496:500	three agricultural waste	477:500	three agricultural waste	477:500	Molecular properties of POM in influent (substrate) and effluent (digestate) of seven full-scale AD plants (three agricultural waste and four food waste digesters) were characterized and compared using solid-state 13C cross-polarization magic angle spinning (CP-MAS) and solution-state 1H,13C heteronuclear single-quantum coherence (HSQC) nuclear magnetic resonance (NMR) spectroscopy.
31390701	3	55	theme	preferential	948:959	arg1	degradation					961:971	a preferential degradation	946:971	a preferential degradation of the carbohydrates over proteins	946:1006	Comparison of the POM structural compositions of substrate and digestate from each AD plant revealed an enrichment of protein structures relative to the carbohydrates in most cases, implying a preferential degradation of the carbohydrates over proteins and/or increase of microbial biomass upon AD of agricultural and food wastes.
31390701	3	56	theme	carbohydrates	980:992	arg1	degradation					961:971	a preferential degradation	946:971	a preferential degradation of the carbohydrates over proteins	946:1006	Comparison of the POM structural compositions of substrate and digestate from each AD plant revealed an enrichment of protein structures relative to the carbohydrates in most cases, implying a preferential degradation of the carbohydrates over proteins and/or increase of microbial biomass upon AD of agricultural and food wastes.
31390701	4	57	theme	molecular	1098:1106	arg1	structures					1108:1117	Distinctive molecular structures	1086:1117	Distinctive molecular structures	1086:1117	Distinctive molecular structures of labile and recalcitrant fractions of POM, subjected to AD, were identified by comparing the NMR spectra of all substrate and digestate POM.
31390701	6	58	from	AD	1655:1656	arg1	lands					1742:1746	digestate-amended arable lands	1717:1746	digestate-amended arable lands	1717:1746	This information serves as a basis to further explore optimization approaches for improving AD of the underutilized POM and the fate of organic matter in digestate-amended arable lands.
31390701	1	59	theme	waste	230:234	arg1	digesters					236:244	agricultural and food waste digesters	208:244	agricultural and food waste digesters	208:244	This study assesses the molecular characteristics of particulate organic matter (POM) in agricultural and food waste digesters and elucidates the molecular properties of the recalcitrant POM fraction, which remains in the digestate after AD process.
31390701	2	60	theme	solid-state	571:581	arg1	angle					612:616	solid-state 13C cross-polarization magic angle spinning (CP-MAS) and solution-state 1H,13C heteronuclear single-quantum coherence (HSQC) nuclear magnetic resonance (NMR) spectroscopy	571:752	solid-state 13C cross-polarization magic angle spinning (CP-MAS) and solution-state 1H,13C heteronuclear single-quantum coherence (HSQC) nuclear magnetic resonance (NMR) spectroscopy	571:752	Molecular properties of POM in influent (substrate) and effluent (digestate) of seven full-scale AD plants (three agricultural waste and four food waste digesters) were characterized and compared using solid-state 13C cross-polarization magic angle spinning (CP-MAS) and solution-state 1H,13C heteronuclear single-quantum coherence (HSQC) nuclear magnetic resonance (NMR) spectroscopy.
31390701	5	61	theme	amino	1494:1498	arg1	acids					1500:1504	amino acids	1494:1504	amino acids	1494:1504	Accordingly, the labile POM fraction in food and agricultural solid wastes is characterized by structural entities of lipids and starch-like carbohydrates, whereas recalcitrant POM structures resemble alkyl and aromatic subunits of amino acids, lignin, and polysaccharides with β-glycosidic linkages.
31390701	4	62	theme	fractions	1146:1154	arg1	structures					1108:1117	Distinctive molecular structures	1086:1117	Distinctive molecular structures	1086:1117	Distinctive molecular structures of labile and recalcitrant fractions of POM, subjected to AD, were identified by comparing the NMR spectra of all substrate and digestate POM.
31390701	5	63	theme	alkyl	1463:1467	arg1	subunits					1482:1489	alkyl and aromatic subunits	1463:1489	alkyl and aromatic subunits of amino acids, lignin, and polysaccharides with β-glycosidic linkages	1463:1560	Accordingly, the labile POM fraction in food and agricultural solid wastes is characterized by structural entities of lipids and starch-like carbohydrates, whereas recalcitrant POM structures resemble alkyl and aromatic subunits of amino acids, lignin, and polysaccharides with β-glycosidic linkages.
31390701	1	64	theme	particulate	172:182	arg1	matter					192:197	particulate organic matter	172:197	particulate organic matter (POM)	172:203	This study assesses the molecular characteristics of particulate organic matter (POM) in agricultural and food waste digesters and elucidates the molecular properties of the recalcitrant POM fraction, which remains in the digestate after AD process.
31390701	1	64	theme	particulate	172:182	arg1	POM					200:202	POM	200:202	POM	200:202	This study assesses the molecular characteristics of particulate organic matter (POM) in agricultural and food waste digesters and elucidates the molecular properties of the recalcitrant POM fraction, which remains in the digestate after AD process.
31390701	4	65	theme	POM	1159:1161	arg1	fractions					1146:1154	labile and recalcitrant fractions	1122:1154	labile and recalcitrant fractions of POM	1122:1161	Distinctive molecular structures of labile and recalcitrant fractions of POM, subjected to AD, were identified by comparing the NMR spectra of all substrate and digestate POM.
31390701	1	66	theme	matter	192:197	arg1	characteristics					153:167	the molecular characteristics	139:167	the molecular characteristics of particulate organic matter (POM)	139:203	This study assesses the molecular characteristics of particulate organic matter (POM) in agricultural and food waste digesters and elucidates the molecular properties of the recalcitrant POM fraction, which remains in the digestate after AD process.
31390701	2	67	theme	cross-polarization	587:604	arg1	angle					612:616	solid-state 13C cross-polarization magic angle spinning (CP-MAS) and solution-state 1H,13C heteronuclear single-quantum coherence (HSQC) nuclear magnetic resonance (NMR) spectroscopy	571:752	solid-state 13C cross-polarization magic angle spinning (CP-MAS) and solution-state 1H,13C heteronuclear single-quantum coherence (HSQC) nuclear magnetic resonance (NMR) spectroscopy	571:752	Molecular properties of POM in influent (substrate) and effluent (digestate) of seven full-scale AD plants (three agricultural waste and four food waste digesters) were characterized and compared using solid-state 13C cross-polarization magic angle spinning (CP-MAS) and solution-state 1H,13C heteronuclear single-quantum coherence (HSQC) nuclear magnetic resonance (NMR) spectroscopy.
31390701	3	68	theme	microbial	1027:1035	arg1	biomass					1037:1043	microbial biomass	1027:1043	microbial biomass upon AD of agricultural and food wastes	1027:1083	Comparison of the POM structural compositions of substrate and digestate from each AD plant revealed an enrichment of protein structures relative to the carbohydrates in most cases, implying a preferential degradation of the carbohydrates over proteins and/or increase of microbial biomass upon AD of agricultural and food wastes.
31390701	3	69	from	plant	841:845	arg1	compositions					788:799	the POM structural compositions	769:799	the POM structural compositions of substrate and digestate from each AD plant	769:845	Comparison of the POM structural compositions of substrate and digestate from each AD plant revealed an enrichment of protein structures relative to the carbohydrates in most cases, implying a preferential degradation of the carbohydrates over proteins and/or increase of microbial biomass upon AD of agricultural and food wastes.
31390701	3	69	from	plant	841:845	arg1	Comparison					755:764	Comparison	755:764	Comparison of the POM structural compositions of substrate and digestate from each AD plant	755:845	Comparison of the POM structural compositions of substrate and digestate from each AD plant revealed an enrichment of protein structures relative to the carbohydrates in most cases, implying a preferential degradation of the carbohydrates over proteins and/or increase of microbial biomass upon AD of agricultural and food wastes.
31390701	5	70	theme	recalcitrant	1426:1437	arg1	structures					1443:1452	recalcitrant POM structures	1426:1452	recalcitrant POM structures	1426:1452	Accordingly, the labile POM fraction in food and agricultural solid wastes is characterized by structural entities of lipids and starch-like carbohydrates, whereas recalcitrant POM structures resemble alkyl and aromatic subunits of amino acids, lignin, and polysaccharides with β-glycosidic linkages.
31390701	2	71	dep	angle	612:616	arg1	spectroscopy					741:752	spinning (CP-MAS) and solution-state 1H,13C heteronuclear single-quantum coherence (HSQC) nuclear magnetic resonance (NMR) spectroscopy	618:752	spectroscopy	741:752	Molecular properties of POM in influent (substrate) and effluent (digestate) of seven full-scale AD plants (three agricultural waste and four food waste digesters) were characterized and compared using solid-state 13C cross-polarization magic angle spinning (CP-MAS) and solution-state 1H,13C heteronuclear single-quantum coherence (HSQC) nuclear magnetic resonance (NMR) spectroscopy.
31390701	2	71	dep	angle	612:616	arg1	CP-MAS					628:633	spinning (CP-MAS) and solution-state 1H,13C heteronuclear single-quantum coherence (HSQC) nuclear magnetic resonance (NMR) spectroscopy	618:752	CP-MAS	628:633	Molecular properties of POM in influent (substrate) and effluent (digestate) of seven full-scale AD plants (three agricultural waste and four food waste digesters) were characterized and compared using solid-state 13C cross-polarization magic angle spinning (CP-MAS) and solution-state 1H,13C heteronuclear single-quantum coherence (HSQC) nuclear magnetic resonance (NMR) spectroscopy.
31390701	2	72	theme	waste	516:520	arg1	digesters					522:530	four food waste digesters	506:530	four food waste digesters	506:530	Molecular properties of POM in influent (substrate) and effluent (digestate) of seven full-scale AD plants (three agricultural waste and four food waste digesters) were characterized and compared using solid-state 13C cross-polarization magic angle spinning (CP-MAS) and solution-state 1H,13C heteronuclear single-quantum coherence (HSQC) nuclear magnetic resonance (NMR) spectroscopy.
31390701	6	73	theme	organic	1699:1705	arg1	matter					1707:1712	organic matter	1699:1712	organic matter	1699:1712	This information serves as a basis to further explore optimization approaches for improving AD of the underutilized POM and the fate of organic matter in digestate-amended arable lands.
31390701	3	74	theme	POM	773:775	arg1	compositions					788:799	the POM structural compositions	769:799	the POM structural compositions of substrate and digestate from each AD plant	769:845	Comparison of the POM structural compositions of substrate and digestate from each AD plant revealed an enrichment of protein structures relative to the carbohydrates in most cases, implying a preferential degradation of the carbohydrates over proteins and/or increase of microbial biomass upon AD of agricultural and food wastes.
31390701	5	75	with	subunits	1482:1489	arg1	linkages					1553:1560	β-glycosidic linkages	1540:1560	β-glycosidic linkages	1540:1560	Accordingly, the labile POM fraction in food and agricultural solid wastes is characterized by structural entities of lipids and starch-like carbohydrates, whereas recalcitrant POM structures resemble alkyl and aromatic subunits of amino acids, lignin, and polysaccharides with β-glycosidic linkages.
31390701	5	76	theme	labile	1279:1284	arg1	fraction					1290:1297	the labile POM fraction	1275:1297	the labile POM fraction in food and agricultural solid wastes	1275:1335	Accordingly, the labile POM fraction in food and agricultural solid wastes is characterized by structural entities of lipids and starch-like carbohydrates, whereas recalcitrant POM structures resemble alkyl and aromatic subunits of amino acids, lignin, and polysaccharides with β-glycosidic linkages.
31390701	3	77	theme	substrate	804:812	arg1	compositions					788:799	the POM structural compositions	769:799	the POM structural compositions of substrate and digestate from each AD plant	769:845	Comparison of the POM structural compositions of substrate and digestate from each AD plant revealed an enrichment of protein structures relative to the carbohydrates in most cases, implying a preferential degradation of the carbohydrates over proteins and/or increase of microbial biomass upon AD of agricultural and food wastes.
31390701	0	78	theme	anaerobic	71:79	arg1	digesters					81:89	full scale anaerobic digesters	60:89	full scale anaerobic digesters	60:89	Molecular characterization of particulate organic matter in full scale anaerobic digesters: An NMR spectroscopy study.
31390701	2	79	theme	plants	469:474	arg1	POM					393:395	POM	393:395	POM in influent (substrate) and effluent (digestate) of seven full-scale AD plants (three agricultural waste and four food waste digesters)	393:531	Molecular properties of POM in influent (substrate) and effluent (digestate) of seven full-scale AD plants (three agricultural waste and four food waste digesters) were characterized and compared using solid-state 13C cross-polarization magic angle spinning (CP-MAS) and solution-state 1H,13C heteronuclear single-quantum coherence (HSQC) nuclear magnetic resonance (NMR) spectroscopy.
31390701	3	80	from	carbohydrates	908:920	arg1	cases					930:934	most cases	925:934	most cases	925:934	Comparison of the POM structural compositions of substrate and digestate from each AD plant revealed an enrichment of protein structures relative to the carbohydrates in most cases, implying a preferential degradation of the carbohydrates over proteins and/or increase of microbial biomass upon AD of agricultural and food wastes.
31390701	2	81	theme	magnetic	716:723	arg1	spectroscopy					741:752	spinning (CP-MAS) and solution-state 1H,13C heteronuclear single-quantum coherence (HSQC) nuclear magnetic resonance (NMR) spectroscopy	618:752	spectroscopy	741:752	Molecular properties of POM in influent (substrate) and effluent (digestate) of seven full-scale AD plants (three agricultural waste and four food waste digesters) were characterized and compared using solid-state 13C cross-polarization magic angle spinning (CP-MAS) and solution-state 1H,13C heteronuclear single-quantum coherence (HSQC) nuclear magnetic resonance (NMR) spectroscopy.
31390701	3	82	theme	compositions	788:799	arg1	Comparison					755:764	Comparison	755:764	Comparison of the POM structural compositions of substrate and digestate from each AD plant	755:845	Comparison of the POM structural compositions of substrate and digestate from each AD plant revealed an enrichment of protein structures relative to the carbohydrates in most cases, implying a preferential degradation of the carbohydrates over proteins and/or increase of microbial biomass upon AD of agricultural and food wastes.
31390701	3	83	theme	structures	881:890	arg1	enrichment					859:868	an enrichment	856:868	an enrichment of protein structures relative to the carbohydrates in most cases	856:934	Comparison of the POM structural compositions of substrate and digestate from each AD plant revealed an enrichment of protein structures relative to the carbohydrates in most cases, implying a preferential degradation of the carbohydrates over proteins and/or increase of microbial biomass upon AD of agricultural and food wastes.
31390701	2	84	theme	full-scale	455:464	arg1	plants					469:474	seven full-scale AD plants	449:474	seven full-scale AD plants (three agricultural waste and four food waste digesters)	449:531	Molecular properties of POM in influent (substrate) and effluent (digestate) of seven full-scale AD plants (three agricultural waste and four food waste digesters) were characterized and compared using solid-state 13C cross-polarization magic angle spinning (CP-MAS) and solution-state 1H,13C heteronuclear single-quantum coherence (HSQC) nuclear magnetic resonance (NMR) spectroscopy.
31390701	0	85	from	characterization	10:25	arg1	digesters					81:89	full scale anaerobic digesters	60:89	full scale anaerobic digesters	60:89	Molecular characterization of particulate organic matter in full scale anaerobic digesters: An NMR spectroscopy study.
31390701	5	86	theme	acids	1500:1504	arg1	subunits					1482:1489	alkyl and aromatic subunits	1463:1489	alkyl and aromatic subunits of amino acids, lignin, and polysaccharides with β-glycosidic linkages	1463:1560	Accordingly, the labile POM fraction in food and agricultural solid wastes is characterized by structural entities of lipids and starch-like carbohydrates, whereas recalcitrant POM structures resemble alkyl and aromatic subunits of amino acids, lignin, and polysaccharides with β-glycosidic linkages.
31390701	0	87	theme	NMR	95:97	arg1	spectroscopy					99:110	An NMR spectroscopy	92:110	Molecular characterization of particulate organic matter in full scale anaerobic digesters: An NMR spectroscopy study.	0:117	Molecular characterization of particulate organic matter in full scale anaerobic digesters: An NMR spectroscopy study.
31390701	2	88	from	influent	400:407	arg1	properties					379:388	Molecular properties	369:388	Molecular properties of POM in influent (substrate) and effluent (digestate) of seven full-scale AD plants (three agricultural waste and four food waste digesters)	369:531	Molecular properties of POM in influent (substrate) and effluent (digestate) of seven full-scale AD plants (three agricultural waste and four food waste digesters) were characterized and compared using solid-state 13C cross-polarization magic angle spinning (CP-MAS) and solution-state 1H,13C heteronuclear single-quantum coherence (HSQC) nuclear magnetic resonance (NMR) spectroscopy.
31390701	5	89	theme	lignin	1507:1512	arg1	subunits					1482:1489	alkyl and aromatic subunits	1463:1489	alkyl and aromatic subunits of amino acids, lignin, and polysaccharides with β-glycosidic linkages	1463:1560	Accordingly, the labile POM fraction in food and agricultural solid wastes is characterized by structural entities of lipids and starch-like carbohydrates, whereas recalcitrant POM structures resemble alkyl and aromatic subunits of amino acids, lignin, and polysaccharides with β-glycosidic linkages.
31390701	5	90	theme	agricultural	1311:1322	arg1	wastes					1330:1335	agricultural solid wastes	1311:1335	agricultural solid wastes	1311:1335	Accordingly, the labile POM fraction in food and agricultural solid wastes is characterized by structural entities of lipids and starch-like carbohydrates, whereas recalcitrant POM structures resemble alkyl and aromatic subunits of amino acids, lignin, and polysaccharides with β-glycosidic linkages.
31390701	3	91	theme	digestate	818:826	arg1	compositions					788:799	the POM structural compositions	769:799	the POM structural compositions of substrate and digestate from each AD plant	769:845	Comparison of the POM structural compositions of substrate and digestate from each AD plant revealed an enrichment of protein structures relative to the carbohydrates in most cases, implying a preferential degradation of the carbohydrates over proteins and/or increase of microbial biomass upon AD of agricultural and food wastes.
31390701	1	92	theme	AD	357:358	arg1	process					360:366	AD process	357:366	AD process	357:366	This study assesses the molecular characteristics of particulate organic matter (POM) in agricultural and food waste digesters and elucidates the molecular properties of the recalcitrant POM fraction, which remains in the digestate after AD process.
31390701	6	93	theme	arable	1735:1740	arg1	lands					1742:1746	digestate-amended arable lands	1717:1746	digestate-amended arable lands	1717:1746	This information serves as a basis to further explore optimization approaches for improving AD of the underutilized POM and the fate of organic matter in digestate-amended arable lands.
31390701	5	94	theme	aromatic	1473:1480	arg1	subunits					1482:1489	alkyl and aromatic subunits	1463:1489	alkyl and aromatic subunits of amino acids, lignin, and polysaccharides with β-glycosidic linkages	1463:1560	Accordingly, the labile POM fraction in food and agricultural solid wastes is characterized by structural entities of lipids and starch-like carbohydrates, whereas recalcitrant POM structures resemble alkyl and aromatic subunits of amino acids, lignin, and polysaccharides with β-glycosidic linkages.
31390701	2	95	theme	single-quantum	676:689	arg1	spectroscopy					741:752	spinning (CP-MAS) and solution-state 1H,13C heteronuclear single-quantum coherence (HSQC) nuclear magnetic resonance (NMR) spectroscopy	618:752	spectroscopy	741:752	Molecular properties of POM in influent (substrate) and effluent (digestate) of seven full-scale AD plants (three agricultural waste and four food waste digesters) were characterized and compared using solid-state 13C cross-polarization magic angle spinning (CP-MAS) and solution-state 1H,13C heteronuclear single-quantum coherence (HSQC) nuclear magnetic resonance (NMR) spectroscopy.
31390701	3	96	theme	wastes	1078:1083	arg1	biomass					1037:1043	microbial biomass	1027:1043	microbial biomass upon AD of agricultural and food wastes	1027:1083	Comparison of the POM structural compositions of substrate and digestate from each AD plant revealed an enrichment of protein structures relative to the carbohydrates in most cases, implying a preferential degradation of the carbohydrates over proteins and/or increase of microbial biomass upon AD of agricultural and food wastes.
31390701	2	97	theme	1H,13C	655:660	arg1	spectroscopy					741:752	spinning (CP-MAS) and solution-state 1H,13C heteronuclear single-quantum coherence (HSQC) nuclear magnetic resonance (NMR) spectroscopy	618:752	spectroscopy	741:752	Molecular properties of POM in influent (substrate) and effluent (digestate) of seven full-scale AD plants (three agricultural waste and four food waste digesters) were characterized and compared using solid-state 13C cross-polarization magic angle spinning (CP-MAS) and solution-state 1H,13C heteronuclear single-quantum coherence (HSQC) nuclear magnetic resonance (NMR) spectroscopy.
31390701	2	98	theme	Molecular	369:377	arg1	properties					379:388	Molecular properties	369:388	Molecular properties of POM in influent (substrate) and effluent (digestate) of seven full-scale AD plants (three agricultural waste and four food waste digesters)	369:531	Molecular properties of POM in influent (substrate) and effluent (digestate) of seven full-scale AD plants (three agricultural waste and four food waste digesters) were characterized and compared using solid-state 13C cross-polarization magic angle spinning (CP-MAS) and solution-state 1H,13C heteronuclear single-quantum coherence (HSQC) nuclear magnetic resonance (NMR) spectroscopy.
31390701	0	99	theme	particulate	30:40	arg1	matter					50:55	particulate organic matter	30:55	particulate organic matter	30:55	Molecular characterization of particulate organic matter in full scale anaerobic digesters: An NMR spectroscopy study.
31390701	6	100	theme	optimization	1617:1628	arg1	approaches					1630:1639	optimization approaches	1617:1639	optimization approaches for improving AD of the underutilized POM and the fate of organic matter in digestate-amended arable lands	1617:1746	This information serves as a basis to further explore optimization approaches for improving AD of the underutilized POM and the fate of organic matter in digestate-amended arable lands.
31390701	0	101	theme	matter	50:55	arg1	characterization					10:25	Molecular characterization	0:25	Molecular characterization of particulate organic matter in full scale anaerobic digesters: An NMR spectroscopy study.	0:117	Molecular characterization of particulate organic matter in full scale anaerobic digesters: An NMR spectroscopy study.
31390701	1	102	theme	recalcitrant	293:304	arg1	fraction					310:317	the recalcitrant POM fraction	289:317	the recalcitrant POM fraction	289:317	This study assesses the molecular characteristics of particulate organic matter (POM) in agricultural and food waste digesters and elucidates the molecular properties of the recalcitrant POM fraction, which remains in the digestate after AD process.
31390701	3	103	theme	most	925:928	arg1	cases					930:934	most cases	925:934	most cases	925:934	Comparison of the POM structural compositions of substrate and digestate from each AD plant revealed an enrichment of protein structures relative to the carbohydrates in most cases, implying a preferential degradation of the carbohydrates over proteins and/or increase of microbial biomass upon AD of agricultural and food wastes.
31390701	0	104	theme	full	60:63	arg1	digesters					81:89	full scale anaerobic digesters	60:89	full scale anaerobic digesters	60:89	Molecular characterization of particulate organic matter in full scale anaerobic digesters: An NMR spectroscopy study.
31390701	1	105	theme	fraction	310:317	arg1	properties					275:284	the molecular properties	261:284	the molecular properties of the recalcitrant POM fraction, which remains in the digestate after AD process	261:366	This study assesses the molecular characteristics of particulate organic matter (POM) in agricultural and food waste digesters and elucidates the molecular properties of the recalcitrant POM fraction, which remains in the digestate after AD process.
31390701	4	106	theme	POM	1257:1259	arg1	spectra					1218:1224	the NMR spectra	1210:1224	the NMR spectra of all substrate and digestate POM	1210:1259	Distinctive molecular structures of labile and recalcitrant fractions of POM, subjected to AD, were identified by comparing the NMR spectra of all substrate and digestate POM.
31390701	1	107	theme	food	225:228	arg1	digesters					236:244	agricultural and food waste digesters	208:244	agricultural and food waste digesters	208:244	This study assesses the molecular characteristics of particulate organic matter (POM) in agricultural and food waste digesters and elucidates the molecular properties of the recalcitrant POM fraction, which remains in the digestate after AD process.
31390701	0	108	dep	characterization	10:25	arg1	study					112:116	An NMR spectroscopy study	92:116	Molecular characterization of particulate organic matter in full scale anaerobic digesters: An NMR spectroscopy study.	0:117	Molecular characterization of particulate organic matter in full scale anaerobic digesters: An NMR spectroscopy study.
31390701	4	109	theme	Distinctive	1086:1096	arg1	structures					1108:1117	Distinctive molecular structures	1086:1117	Distinctive molecular structures	1086:1117	Distinctive molecular structures of labile and recalcitrant fractions of POM, subjected to AD, were identified by comparing the NMR spectra of all substrate and digestate POM.
31390701	5	110	theme	structural	1357:1366	arg1	entities					1368:1375	structural entities	1357:1375	structural entities of lipids and starch-like carbohydrates	1357:1415	Accordingly, the labile POM fraction in food and agricultural solid wastes is characterized by structural entities of lipids and starch-like carbohydrates, whereas recalcitrant POM structures resemble alkyl and aromatic subunits of amino acids, lignin, and polysaccharides with β-glycosidic linkages.
30419468	1	0	theme	dissolved	96:104	arg1	DOM					122:124	DOM	122:124	DOM	122:124	Isolating dissolved organic matter (DOM) is a preliminary step that improves the accuracy of its characterization.
30419468	1	0	theme	dissolved	96:104	arg1	matter					114:119	dissolved organic matter	96:119	dissolved organic matter (DOM)	96:125	Isolating dissolved organic matter (DOM) is a preliminary step that improves the accuracy of its characterization.
30419468	6	1	theme	polyhydroxy	918:928	arg1	aromatics					930:938	polyhydroxy aromatics	918:938	polyhydroxy aromatics	918:938	Py-GC/MS revealed that Fraction 1 consisted of evenly distributed organic matter in order polysaccharides, proteins, polyhydroxy aromatics, lignins, and lipids.
30419468	6	2	from	matter	875:880	arg1	lipids					954:959	lipids	954:959	lipids	954:959	Py-GC/MS revealed that Fraction 1 consisted of evenly distributed organic matter in order polysaccharides, proteins, polyhydroxy aromatics, lignins, and lipids.
30419468	6	2	from	matter	875:880	arg1	lignins					941:947	lignins	941:947	lignins	941:947	Py-GC/MS revealed that Fraction 1 consisted of evenly distributed organic matter in order polysaccharides, proteins, polyhydroxy aromatics, lignins, and lipids.
30419468	6	2	from	matter	875:880	arg1	proteins					908:915	proteins	908:915	proteins	908:915	Py-GC/MS revealed that Fraction 1 consisted of evenly distributed organic matter in order polysaccharides, proteins, polyhydroxy aromatics, lignins, and lipids.
30419468	6	2	from	matter	875:880	arg1	aromatics					930:938	polyhydroxy aromatics	918:938	polyhydroxy aromatics	918:938	Py-GC/MS revealed that Fraction 1 consisted of evenly distributed organic matter in order polysaccharides, proteins, polyhydroxy aromatics, lignins, and lipids.
30419468	6	2	from	matter	875:880	arg1	polysaccharides					891:905	order polysaccharides	885:905	order polysaccharides	885:905	Py-GC/MS revealed that Fraction 1 consisted of evenly distributed organic matter in order polysaccharides, proteins, polyhydroxy aromatics, lignins, and lipids.
30419468	4	3	theme	size	520:523	arg1	SEC					551:553	SEC	551:553	SEC	551:553	The homogeneity of each fraction was estimated by analytical size exclusion chromatography (SEC) and fluorescence excitation-emission matrix (FEEM).
30419468	4	3	theme	size	520:523	arg1	chromatography					535:548	analytical size exclusion chromatography	509:548	analytical size exclusion chromatography (SEC)	509:554	The homogeneity of each fraction was estimated by analytical size exclusion chromatography (SEC) and fluorescence excitation-emission matrix (FEEM).
30419468	5	4	theme	liquid	670:675	arg1	LC-OCD					718:723	LC-OCD	718:723	LC-OCD	718:723	Pyrolysis gas chromatography-mass spectrometry (Py-GC/MS) and liquid chromatography-organic carbon detection (LC-OCD) were used to characterize the physicochemical properties of each fraction.
30419468	5	4	theme	liquid	670:675	arg1	detection					707:715	liquid chromatography-organic carbon detection	670:715	liquid chromatography-organic carbon detection (LC-OCD)	670:724	Pyrolysis gas chromatography-mass spectrometry (Py-GC/MS) and liquid chromatography-organic carbon detection (LC-OCD) were used to characterize the physicochemical properties of each fraction.
30419468	6	5	theme	distributed	855:865	arg1	matter					875:880	evenly distributed organic matter	848:880	evenly distributed organic matter in order polysaccharides, proteins, polyhydroxy aromatics, lignins, and lipids	848:959	Py-GC/MS revealed that Fraction 1 consisted of evenly distributed organic matter in order polysaccharides, proteins, polyhydroxy aromatics, lignins, and lipids.
30419468	8	6	theme	similar	1171:1177	arg1	compositions					1199:1210	similar organic carbon (OC) compositions	1171:1210	similar organic carbon (OC) compositions	1171:1210	The LC-OCD results showed that Fractions 1 and 2 had similar organic carbon (OC) compositions: a humic substance (ca. 37%), building blocks (ca. 10%), and neutrals (ca. 37%), whereas Fraction 3 contained a high proportion of neutrals (62%).
30419468	4	7	theme	analytical	509:518	arg1	SEC					551:553	SEC	551:553	SEC	551:553	The homogeneity of each fraction was estimated by analytical size exclusion chromatography (SEC) and fluorescence excitation-emission matrix (FEEM).
30419468	4	7	theme	analytical	509:518	arg1	chromatography					535:548	analytical size exclusion chromatography	509:548	analytical size exclusion chromatography (SEC)	509:554	The homogeneity of each fraction was estimated by analytical size exclusion chromatography (SEC) and fluorescence excitation-emission matrix (FEEM).
30419468	5	8	theme	chromatography-organic	677:698	arg1	LC-OCD					718:723	LC-OCD	718:723	LC-OCD	718:723	Pyrolysis gas chromatography-mass spectrometry (Py-GC/MS) and liquid chromatography-organic carbon detection (LC-OCD) were used to characterize the physicochemical properties of each fraction.
30419468	5	8	theme	chromatography-organic	677:698	arg1	detection					707:715	liquid chromatography-organic carbon detection	670:715	liquid chromatography-organic carbon detection (LC-OCD)	670:724	Pyrolysis gas chromatography-mass spectrometry (Py-GC/MS) and liquid chromatography-organic carbon detection (LC-OCD) were used to characterize the physicochemical properties of each fraction.
30419468	5	9	theme	Pyrolysis	608:616	arg1	Py-GC/MS					656:663	Py-GC/MS	656:663	Py-GC/MS	656:663	Pyrolysis gas chromatography-mass spectrometry (Py-GC/MS) and liquid chromatography-organic carbon detection (LC-OCD) were used to characterize the physicochemical properties of each fraction.
30419468	5	9	theme	Pyrolysis	608:616	arg1	spectrometry					642:653	Pyrolysis gas chromatography-mass spectrometry	608:653	Pyrolysis gas chromatography-mass spectrometry (Py-GC/MS)	608:664	Pyrolysis gas chromatography-mass spectrometry (Py-GC/MS) and liquid chromatography-organic carbon detection (LC-OCD) were used to characterize the physicochemical properties of each fraction.
30419468	5	10	theme	physicochemical	756:770	arg1	properties					772:781	the physicochemical properties	752:781	the physicochemical properties of each fraction	752:798	Pyrolysis gas chromatography-mass spectrometry (Py-GC/MS) and liquid chromatography-organic carbon detection (LC-OCD) were used to characterize the physicochemical properties of each fraction.
30419468	5	11	theme	carbon	700:705	arg1	LC-OCD					718:723	LC-OCD	718:723	LC-OCD	718:723	Pyrolysis gas chromatography-mass spectrometry (Py-GC/MS) and liquid chromatography-organic carbon detection (LC-OCD) were used to characterize the physicochemical properties of each fraction.
30419468	5	11	theme	carbon	700:705	arg1	detection					707:715	liquid chromatography-organic carbon detection	670:715	liquid chromatography-organic carbon detection (LC-OCD)	670:724	Pyrolysis gas chromatography-mass spectrometry (Py-GC/MS) and liquid chromatography-organic carbon detection (LC-OCD) were used to characterize the physicochemical properties of each fraction.
30419468	5	12	theme	gas	618:620	arg1	Py-GC/MS					656:663	Py-GC/MS	656:663	Py-GC/MS	656:663	Pyrolysis gas chromatography-mass spectrometry (Py-GC/MS) and liquid chromatography-organic carbon detection (LC-OCD) were used to characterize the physicochemical properties of each fraction.
30419468	5	12	theme	gas	618:620	arg1	spectrometry					642:653	Pyrolysis gas chromatography-mass spectrometry	608:653	Pyrolysis gas chromatography-mass spectrometry (Py-GC/MS)	608:664	Pyrolysis gas chromatography-mass spectrometry (Py-GC/MS) and liquid chromatography-organic carbon detection (LC-OCD) were used to characterize the physicochemical properties of each fraction.
30419468	9	13	theme	DOM	1400:1402	arg1	characteristics					1404:1418	the distinct DOM characteristics	1387:1418	the distinct DOM characteristics in each fraction	1387:1435	In the fouling experiments, the distinct DOM characteristics in each fraction resulted in different declining flux behaviors, ranked as: Fraction 2 > Fraction 1 > Fraction 3.
30419468	8	14	theme	carbon	1187:1192	arg1	compositions					1199:1210	similar organic carbon (OC) compositions	1171:1210	similar organic carbon (OC) compositions	1171:1210	The LC-OCD results showed that Fractions 1 and 2 had similar organic carbon (OC) compositions: a humic substance (ca. 37%), building blocks (ca. 10%), and neutrals (ca. 37%), whereas Fraction 3 contained a high proportion of neutrals (62%).
30419468	5	15	theme	chromatography-mass	622:640	arg1	Py-GC/MS					656:663	Py-GC/MS	656:663	Py-GC/MS	656:663	Pyrolysis gas chromatography-mass spectrometry (Py-GC/MS) and liquid chromatography-organic carbon detection (LC-OCD) were used to characterize the physicochemical properties of each fraction.
30419468	5	15	theme	chromatography-mass	622:640	arg1	spectrometry					642:653	Pyrolysis gas chromatography-mass spectrometry	608:653	Pyrolysis gas chromatography-mass spectrometry (Py-GC/MS)	608:664	Pyrolysis gas chromatography-mass spectrometry (Py-GC/MS) and liquid chromatography-organic carbon detection (LC-OCD) were used to characterize the physicochemical properties of each fraction.
30419468	8	16	contain	had	1167:1169	arg2	substance					1221:1229	a humic substance	1213:1229	a humic substance (ca. 37%)	1213:1239	The LC-OCD results showed that Fractions 1 and 2 had similar organic carbon (OC) compositions: a humic substance (ca. 37%), building blocks (ca. 10%), and neutrals (ca. 37%), whereas Fraction 3 contained a high proportion of neutrals (62%).
30419468	8	16	contain	had	1167:1169	arg2	%					1265:1265	10%	1263:1265	10%	1263:1265	The LC-OCD results showed that Fractions 1 and 2 had similar organic carbon (OC) compositions: a humic substance (ca. 37%), building blocks (ca. 10%), and neutrals (ca. 37%), whereas Fraction 3 contained a high proportion of neutrals (62%).
30419468	8	16	contain	had	1167:1169	arg2	blocks					1251:1256	building blocks	1242:1256	building blocks (ca. 10%)	1242:1266	The LC-OCD results showed that Fractions 1 and 2 had similar organic carbon (OC) compositions: a humic substance (ca. 37%), building blocks (ca. 10%), and neutrals (ca. 37%), whereas Fraction 3 contained a high proportion of neutrals (62%).
30419468	8	16	contain	had	1167:1169	arg2	neutrals					1273:1280	neutrals	1273:1280	neutrals (ca. 37%)	1273:1290	The LC-OCD results showed that Fractions 1 and 2 had similar organic carbon (OC) compositions: a humic substance (ca. 37%), building blocks (ca. 10%), and neutrals (ca. 37%), whereas Fraction 3 contained a high proportion of neutrals (62%).
30419468	8	16	contain	had	1167:1169	arg1	Fractions					1149:1157	Fractions 1 and 2	1149:1165	Fractions	1149:1157	The LC-OCD results showed that Fractions 1 and 2 had similar organic carbon (OC) compositions: a humic substance (ca. 37%), building blocks (ca. 10%), and neutrals (ca. 37%), whereas Fraction 3 contained a high proportion of neutrals (62%).
30419468	8	16	contain	had	1167:1169	arg2	compositions					1199:1210	similar organic carbon (OC) compositions	1171:1210	similar organic carbon (OC) compositions	1171:1210	The LC-OCD results showed that Fractions 1 and 2 had similar organic carbon (OC) compositions: a humic substance (ca. 37%), building blocks (ca. 10%), and neutrals (ca. 37%), whereas Fraction 3 contained a high proportion of neutrals (62%).
30419468	8	16	contain	had	1167:1169	arg1	2					1165:1165	2	1165:1165	2	1165:1165	The LC-OCD results showed that Fractions 1 and 2 had similar organic carbon (OC) compositions: a humic substance (ca. 37%), building blocks (ca. 10%), and neutrals (ca. 37%), whereas Fraction 3 contained a high proportion of neutrals (62%).
30419468	8	17	contain	contained	1312:1320	arg2	proportion					1329:1338	a high proportion	1322:1338	a high proportion of neutrals (62%)	1322:1356	The LC-OCD results showed that Fractions 1 and 2 had similar organic carbon (OC) compositions: a humic substance (ca. 37%), building blocks (ca. 10%), and neutrals (ca. 37%), whereas Fraction 3 contained a high proportion of neutrals (62%).
30419468	8	17	contain	contained	1312:1320	arg1	Fraction					1301:1308	Fraction 3	1301:1310	Fraction 3	1301:1310	The LC-OCD results showed that Fractions 1 and 2 had similar organic carbon (OC) compositions: a humic substance (ca. 37%), building blocks (ca. 10%), and neutrals (ca. 37%), whereas Fraction 3 contained a high proportion of neutrals (62%).
30419468	0	18	theme	dissolved	42:50	arg1	matter					60:65	dissolved organic matter	42:65	dissolved organic matter	42:65	Evaluating membrane fouling potentials of dissolved organic matter in brackish water.
30419468	9	19	theme	declining	1459:1467	arg1	behaviors					1474:1482	different declining flux behaviors	1449:1482	different declining flux behaviors	1449:1482	In the fouling experiments, the distinct DOM characteristics in each fraction resulted in different declining flux behaviors, ranked as: Fraction 2 > Fraction 1 > Fraction 3.
30419468	2	20	from	DOM	216:218	arg1	water					232:236	brackish water	223:236	brackish water	223:236	In this study, DOM in brackish water was clearly separated and evaluated by multiple characterization analyses.
30419468	3	21	theme	molecular	443:451	arg1	size					453:456	molecular size	443:456	molecular size	443:456	The sample was divided into three fractions by preparative high-performance liquid chromatography (preparative HPLC) according to molecular size.
30419468	0	22	theme	membrane	11:18	arg1	potentials					28:37	membrane fouling potentials	11:37	membrane fouling potentials of dissolved organic matter in brackish water	11:83	Evaluating membrane fouling potentials of dissolved organic matter in brackish water.
30419468	4	23	theme	fraction	483:490	arg1	homogeneity					463:473	The homogeneity	459:473	The homogeneity of each fraction	459:490	The homogeneity of each fraction was estimated by analytical size exclusion chromatography (SEC) and fluorescence excitation-emission matrix (FEEM).
30419468	8	24	dep	%	1238:1238	arg1	ca.					1232:1234	ca.	1232:1234	ca.	1232:1234	The LC-OCD results showed that Fractions 1 and 2 had similar organic carbon (OC) compositions: a humic substance (ca. 37%), building blocks (ca. 10%), and neutrals (ca. 37%), whereas Fraction 3 contained a high proportion of neutrals (62%).
30419468	3	25	theme	liquid	389:394	arg1	chromatography					396:409	preparative high-performance liquid chromatography	360:409	preparative high-performance liquid chromatography (preparative HPLC) according to molecular size	360:456	The sample was divided into three fractions by preparative high-performance liquid chromatography (preparative HPLC) according to molecular size.
30419468	3	25	theme	liquid	389:394	arg1	HPLC					424:427	preparative HPLC	412:427	preparative HPLC	412:427	The sample was divided into three fractions by preparative high-performance liquid chromatography (preparative HPLC) according to molecular size.
30419468	9	26	theme	flux	1469:1472	arg1	behaviors					1474:1482	different declining flux behaviors	1449:1482	different declining flux behaviors	1449:1482	In the fouling experiments, the distinct DOM characteristics in each fraction resulted in different declining flux behaviors, ranked as: Fraction 2 > Fraction 1 > Fraction 3.
30419468	2	27	theme	characterization	286:301	arg1	analyses					303:310	multiple characterization analyses	277:310	multiple characterization analyses	277:310	In this study, DOM in brackish water was clearly separated and evaluated by multiple characterization analyses.
30419468	8	28	theme	humic	1215:1219	arg1	substance					1221:1229	a humic substance	1213:1229	a humic substance (ca. 37%)	1213:1239	The LC-OCD results showed that Fractions 1 and 2 had similar organic carbon (OC) compositions: a humic substance (ca. 37%), building blocks (ca. 10%), and neutrals (ca. 37%), whereas Fraction 3 contained a high proportion of neutrals (62%).
30419468	8	29	theme	organic	1179:1185	arg1	compositions					1199:1210	similar organic carbon (OC) compositions	1171:1210	similar organic carbon (OC) compositions	1171:1210	The LC-OCD results showed that Fractions 1 and 2 had similar organic carbon (OC) compositions: a humic substance (ca. 37%), building blocks (ca. 10%), and neutrals (ca. 37%), whereas Fraction 3 contained a high proportion of neutrals (62%).
30419468	2	30	theme	multiple	277:284	arg1	analyses					303:310	multiple characterization analyses	277:310	multiple characterization analyses	277:310	In this study, DOM in brackish water was clearly separated and evaluated by multiple characterization analyses.
30419468	8	31	dep	%	1289:1289	arg1	ca.					1283:1285	ca.	1283:1285	ca.	1283:1285	The LC-OCD results showed that Fractions 1 and 2 had similar organic carbon (OC) compositions: a humic substance (ca. 37%), building blocks (ca. 10%), and neutrals (ca. 37%), whereas Fraction 3 contained a high proportion of neutrals (62%).
30419468	3	32	theme	preparative	360:370	arg1	chromatography					396:409	preparative high-performance liquid chromatography	360:409	preparative high-performance liquid chromatography (preparative HPLC) according to molecular size	360:456	The sample was divided into three fractions by preparative high-performance liquid chromatography (preparative HPLC) according to molecular size.
30419468	3	32	theme	preparative	360:370	arg1	HPLC					424:427	preparative HPLC	412:427	preparative HPLC	412:427	The sample was divided into three fractions by preparative high-performance liquid chromatography (preparative HPLC) according to molecular size.
30419468	4	33	theme	excitation-emission	573:591	arg1	FEEM					601:604	FEEM	601:604	FEEM	601:604	The homogeneity of each fraction was estimated by analytical size exclusion chromatography (SEC) and fluorescence excitation-emission matrix (FEEM).
30419468	4	33	theme	excitation-emission	573:591	arg1	matrix					593:598	fluorescence excitation-emission matrix	560:598	fluorescence excitation-emission matrix (FEEM)	560:605	The homogeneity of each fraction was estimated by analytical size exclusion chromatography (SEC) and fluorescence excitation-emission matrix (FEEM).
30419468	9	34	theme	different	1449:1457	arg1	behaviors					1474:1482	different declining flux behaviors	1449:1482	different declining flux behaviors	1449:1482	In the fouling experiments, the distinct DOM characteristics in each fraction resulted in different declining flux behaviors, ranked as: Fraction 2 > Fraction 1 > Fraction 3.
30419468	1	35	theme	characterization	183:198	arg1	accuracy					167:174	the accuracy	163:174	the accuracy of its characterization	163:198	Isolating dissolved organic matter (DOM) is a preliminary step that improves the accuracy of its characterization.
30419468	3	36	theme	preparative	412:422	arg1	chromatography					396:409	preparative high-performance liquid chromatography	360:409	preparative high-performance liquid chromatography (preparative HPLC) according to molecular size	360:456	The sample was divided into three fractions by preparative high-performance liquid chromatography (preparative HPLC) according to molecular size.
30419468	3	36	theme	preparative	412:422	arg1	HPLC					424:427	preparative HPLC	412:427	preparative HPLC	412:427	The sample was divided into three fractions by preparative high-performance liquid chromatography (preparative HPLC) according to molecular size.
30419468	4	37	theme	fluorescence	560:571	arg1	FEEM					601:604	FEEM	601:604	FEEM	601:604	The homogeneity of each fraction was estimated by analytical size exclusion chromatography (SEC) and fluorescence excitation-emission matrix (FEEM).
30419468	4	37	theme	fluorescence	560:571	arg1	matrix					593:598	fluorescence excitation-emission matrix	560:598	fluorescence excitation-emission matrix (FEEM)	560:605	The homogeneity of each fraction was estimated by analytical size exclusion chromatography (SEC) and fluorescence excitation-emission matrix (FEEM).
30419468	9	38	from	characteristics	1404:1418	arg1	fraction					1428:1435	each fraction	1423:1435	each fraction	1423:1435	In the fouling experiments, the distinct DOM characteristics in each fraction resulted in different declining flux behaviors, ranked as: Fraction 2 > Fraction 1 > Fraction 3.
30419468	9	39	theme	fouling	1366:1372	arg1	experiments					1374:1384	the fouling experiments	1362:1384	the fouling experiments	1362:1384	In the fouling experiments, the distinct DOM characteristics in each fraction resulted in different declining flux behaviors, ranked as: Fraction 2 > Fraction 1 > Fraction 3.
30419468	7	40	theme	proteins	1043:1050	arg1	lipids					1017:1022	dominant lipids	1008:1022	dominant lipids	1008:1022	However, Fraction 2 was primarily composed of dominant lipids and low portion of proteins, and Fraction 3 was composed predominantly of lignins and lipids.
30419468	7	40	theme	proteins	1043:1050	arg1	portion					1032:1038	low portion	1028:1038	low portion of proteins	1028:1050	However, Fraction 2 was primarily composed of dominant lipids and low portion of proteins, and Fraction 3 was composed predominantly of lignins and lipids.
30419468	8	41	theme	OC	1195:1196	arg1	compositions					1199:1210	similar organic carbon (OC) compositions	1171:1210	similar organic carbon (OC) compositions	1171:1210	The LC-OCD results showed that Fractions 1 and 2 had similar organic carbon (OC) compositions: a humic substance (ca. 37%), building blocks (ca. 10%), and neutrals (ca. 37%), whereas Fraction 3 contained a high proportion of neutrals (62%).
30419468	0	42	theme	matter	60:65	arg1	potentials					28:37	membrane fouling potentials	11:37	membrane fouling potentials of dissolved organic matter in brackish water	11:83	Evaluating membrane fouling potentials of dissolved organic matter in brackish water.
30419468	4	43	theme	exclusion	525:533	arg1	SEC					551:553	SEC	551:553	SEC	551:553	The homogeneity of each fraction was estimated by analytical size exclusion chromatography (SEC) and fluorescence excitation-emission matrix (FEEM).
30419468	4	43	theme	exclusion	525:533	arg1	chromatography					535:548	analytical size exclusion chromatography	509:548	analytical size exclusion chromatography (SEC)	509:554	The homogeneity of each fraction was estimated by analytical size exclusion chromatography (SEC) and fluorescence excitation-emission matrix (FEEM).
30419468	5	44	theme	fraction	791:798	arg1	properties					772:781	the physicochemical properties	752:781	the physicochemical properties of each fraction	752:798	Pyrolysis gas chromatography-mass spectrometry (Py-GC/MS) and liquid chromatography-organic carbon detection (LC-OCD) were used to characterize the physicochemical properties of each fraction.
30419468	8	45	theme	high	1324:1327	arg1	proportion					1329:1338	a high proportion	1322:1338	a high proportion of neutrals (62%)	1322:1356	The LC-OCD results showed that Fractions 1 and 2 had similar organic carbon (OC) compositions: a humic substance (ca. 37%), building blocks (ca. 10%), and neutrals (ca. 37%), whereas Fraction 3 contained a high proportion of neutrals (62%).
30419468	0	46	theme	organic	52:58	arg1	matter					60:65	dissolved organic matter	42:65	dissolved organic matter	42:65	Evaluating membrane fouling potentials of dissolved organic matter in brackish water.
30419468	8	47	theme	LC-OCD	1122:1127	arg1	results					1129:1135	The LC-OCD results	1118:1135	The LC-OCD results	1118:1135	The LC-OCD results showed that Fractions 1 and 2 had similar organic carbon (OC) compositions: a humic substance (ca. 37%), building blocks (ca. 10%), and neutrals (ca. 37%), whereas Fraction 3 contained a high proportion of neutrals (62%).
30419468	6	48	theme	order	885:889	arg1	polysaccharides					891:905	order polysaccharides	885:905	order polysaccharides	885:905	Py-GC/MS revealed that Fraction 1 consisted of evenly distributed organic matter in order polysaccharides, proteins, polyhydroxy aromatics, lignins, and lipids.
30419468	9	49	theme	2 > Fraction	1505:1516	arg1	1 > Fraction					1518:1529	Fraction 2 > Fraction 1 > Fraction 3	1496:1531	Fraction 2 > Fraction 1 > Fraction 3	1496:1531	In the fouling experiments, the distinct DOM characteristics in each fraction resulted in different declining flux behaviors, ranked as: Fraction 2 > Fraction 1 > Fraction 3.
30419468	8	50	theme	neutrals	1343:1350	arg1	proportion					1329:1338	a high proportion	1322:1338	a high proportion of neutrals (62%)	1322:1356	The LC-OCD results showed that Fractions 1 and 2 had similar organic carbon (OC) compositions: a humic substance (ca. 37%), building blocks (ca. 10%), and neutrals (ca. 37%), whereas Fraction 3 contained a high proportion of neutrals (62%).
30419468	9	51	theme	Fraction	1496:1503	arg1	1 > Fraction					1518:1529	Fraction 2 > Fraction 1 > Fraction 3	1496:1531	Fraction 2 > Fraction 1 > Fraction 3	1496:1531	In the fouling experiments, the distinct DOM characteristics in each fraction resulted in different declining flux behaviors, ranked as: Fraction 2 > Fraction 1 > Fraction 3.
30419468	8	52	dep	%	1265:1265	arg1	ca.					1259:1261	ca.	1259:1261	ca.	1259:1261	The LC-OCD results showed that Fractions 1 and 2 had similar organic carbon (OC) compositions: a humic substance (ca. 37%), building blocks (ca. 10%), and neutrals (ca. 37%), whereas Fraction 3 contained a high proportion of neutrals (62%).
30419468	9	53	theme	distinct	1391:1398	arg1	characteristics					1404:1418	the distinct DOM characteristics	1387:1418	the distinct DOM characteristics in each fraction	1387:1435	In the fouling experiments, the distinct DOM characteristics in each fraction resulted in different declining flux behaviors, ranked as: Fraction 2 > Fraction 1 > Fraction 3.
30419468	1	54	theme	organic	106:112	arg1	DOM					122:124	DOM	122:124	DOM	122:124	Isolating dissolved organic matter (DOM) is a preliminary step that improves the accuracy of its characterization.
30419468	1	54	theme	organic	106:112	arg1	matter					114:119	dissolved organic matter	96:119	dissolved organic matter (DOM)	96:125	Isolating dissolved organic matter (DOM) is a preliminary step that improves the accuracy of its characterization.
30419468	8	55	theme	building	1242:1249	arg1	%					1265:1265	10%	1263:1265	10%	1263:1265	The LC-OCD results showed that Fractions 1 and 2 had similar organic carbon (OC) compositions: a humic substance (ca. 37%), building blocks (ca. 10%), and neutrals (ca. 37%), whereas Fraction 3 contained a high proportion of neutrals (62%).
30419468	8	55	theme	building	1242:1249	arg1	blocks					1251:1256	building blocks	1242:1256	building blocks (ca. 10%)	1242:1266	The LC-OCD results showed that Fractions 1 and 2 had similar organic carbon (OC) compositions: a humic substance (ca. 37%), building blocks (ca. 10%), and neutrals (ca. 37%), whereas Fraction 3 contained a high proportion of neutrals (62%).
30419468	1	56	theme	preliminary	132:142	arg1	step					144:147	a preliminary step	130:147	a preliminary step that improves the accuracy of its characterization	130:198	Isolating dissolved organic matter (DOM) is a preliminary step that improves the accuracy of its characterization.
30419468	9	57	dep	resulted	1437:1444	arg1	ranked					1485:1490	ranked	1485:1490	ranked as: Fraction 2 > Fraction 1 > Fraction 3	1485:1531	In the fouling experiments, the distinct DOM characteristics in each fraction resulted in different declining flux behaviors, ranked as: Fraction 2 > Fraction 1 > Fraction 3.
30419468	0	58	theme	brackish	70:77	arg1	water					79:83	brackish water	70:83	brackish water	70:83	Evaluating membrane fouling potentials of dissolved organic matter in brackish water.
30419468	6	59	theme	organic	867:873	arg1	matter					875:880	evenly distributed organic matter	848:880	evenly distributed organic matter in order polysaccharides, proteins, polyhydroxy aromatics, lignins, and lipids	848:959	Py-GC/MS revealed that Fraction 1 consisted of evenly distributed organic matter in order polysaccharides, proteins, polyhydroxy aromatics, lignins, and lipids.
30419468	2	60	theme	brackish	223:230	arg1	water					232:236	brackish water	223:236	brackish water	223:236	In this study, DOM in brackish water was clearly separated and evaluated by multiple characterization analyses.
30419468	7	61	theme	low	1028:1030	arg1	portion					1032:1038	low portion	1028:1038	low portion of proteins	1028:1050	However, Fraction 2 was primarily composed of dominant lipids and low portion of proteins, and Fraction 3 was composed predominantly of lignins and lipids.
30419468	4	62	gly	homogeneity	463:473	arg1	fraction					483:490	each fraction	478:490	each fraction	478:490	The homogeneity of each fraction was estimated by analytical size exclusion chromatography (SEC) and fluorescence excitation-emission matrix (FEEM).
30419468	5	63	used	used	731:734	arg2	spectrometry					642:653	Pyrolysis gas chromatography-mass spectrometry	608:653	Pyrolysis gas chromatography-mass spectrometry (Py-GC/MS)	608:664	Pyrolysis gas chromatography-mass spectrometry (Py-GC/MS) and liquid chromatography-organic carbon detection (LC-OCD) were used to characterize the physicochemical properties of each fraction.
30419468	5	63	used	used	731:734	arg2	Py-GC/MS					656:663	Py-GC/MS	656:663	Py-GC/MS	656:663	Pyrolysis gas chromatography-mass spectrometry (Py-GC/MS) and liquid chromatography-organic carbon detection (LC-OCD) were used to characterize the physicochemical properties of each fraction.
30419468	5	63	used	used	731:734	arg2	LC-OCD					718:723	LC-OCD	718:723	LC-OCD	718:723	Pyrolysis gas chromatography-mass spectrometry (Py-GC/MS) and liquid chromatography-organic carbon detection (LC-OCD) were used to characterize the physicochemical properties of each fraction.
30419468	5	63	used	used	731:734	arg2	detection					707:715	liquid chromatography-organic carbon detection	670:715	liquid chromatography-organic carbon detection (LC-OCD)	670:724	Pyrolysis gas chromatography-mass spectrometry (Py-GC/MS) and liquid chromatography-organic carbon detection (LC-OCD) were used to characterize the physicochemical properties of each fraction.
30419468	0	64	from	potentials	28:37	arg1	water					79:83	brackish water	70:83	brackish water	70:83	Evaluating membrane fouling potentials of dissolved organic matter in brackish water.
30419468	0	65	theme	fouling	20:26	arg1	potentials					28:37	membrane fouling potentials	11:37	membrane fouling potentials of dissolved organic matter in brackish water	11:83	Evaluating membrane fouling potentials of dissolved organic matter in brackish water.
30419468	7	66	theme	dominant	1008:1015	arg1	lipids					1017:1022	dominant lipids	1008:1022	dominant lipids	1008:1022	However, Fraction 2 was primarily composed of dominant lipids and low portion of proteins, and Fraction 3 was composed predominantly of lignins and lipids.
30419468	3	67	theme	high-performance	372:387	arg1	chromatography					396:409	preparative high-performance liquid chromatography	360:409	preparative high-performance liquid chromatography (preparative HPLC) according to molecular size	360:456	The sample was divided into three fractions by preparative high-performance liquid chromatography (preparative HPLC) according to molecular size.
30419468	3	67	theme	high-performance	372:387	arg1	HPLC					424:427	preparative HPLC	412:427	preparative HPLC	412:427	The sample was divided into three fractions by preparative high-performance liquid chromatography (preparative HPLC) according to molecular size.
31572329	10	0	theme	amino	1806:1810	arg1	acids					1812:1816	amino acids	1806:1816	amino acids	1806:1816	Consistent with these changes, serum metabolites enriched at the LP stage were associated with the metabolism of amino acids and vitamins.
31572329	3	1	from	contribution	454:465	arg1	microbiome					433:442	the gut microbiome	425:442	the gut microbiome	425:442	However, to our knowledge, changes in the gut microbiome and their contribution to the shifts in host metabolism around parturition have not been investigated in pigs.
31572329	14	2	theme	lipid	2290:2294	arg1	metabolism					2296:2305	lipid metabolism	2290:2305	lipid metabolism	2290:2305	These benefit the lactation of sows by providing energy from lipid metabolism for milk production.
31572329	2	3	theme	host	260:263	arg1	hormones					265:272	host hormones	260:272	host hormones	260:272	Substantial changes in host hormones, metabolism, and immunity around the parturition period may be accompanied by alterations in the gut microbiome.
31572329	7	4	theme	Lactobacillus	1308:1320	arg1	abundance					1273:1281	the relative abundance	1260:1281	the relative abundance of the predominant genus Lactobacillus	1260:1320	The ratio of Bacteroidetes to Firmicutes, and the relative abundance of Prevotella significantly decreased, whilst the relative abundance of the predominant genus Lactobacillus significantly increased from LP to PO state.
31572329	12	5	theme	Lactobacillus	2015:2027	arg1	richness					1947:1954	richness	1947:1954	richness	1947:1954	We further identified that the richness and β-diversity of the gut microbiota and the abundance of Lactobacillus accounted for shifts in the levels of bile acid metabolites associated with lipid metabolism.
31572329	12	5	theme	Lactobacillus	2015:2027	arg1	abundance					2002:2010	the abundance	1998:2010	the abundance of Lactobacillus	1998:2027	We further identified that the richness and β-diversity of the gut microbiota and the abundance of Lactobacillus accounted for shifts in the levels of bile acid metabolites associated with lipid metabolism.
31572329	12	5	theme	Lactobacillus	2015:2027	arg1	β-diversity					1960:1970	β-diversity	1960:1970	β-diversity	1960:1970	We further identified that the richness and β-diversity of the gut microbiota and the abundance of Lactobacillus accounted for shifts in the levels of bile acid metabolites associated with lipid metabolism.
31572329	3	6	theme	host	484:487	arg1	metabolism					489:498	host metabolism	484:498	host metabolism around parturition	484:517	However, to our knowledge, changes in the gut microbiome and their contribution to the shifts in host metabolism around parturition have not been investigated in pigs.
31572329	1	7	theme	due	164:166	arg1	mammals					156:162	mammals	156:162	mammals due to its contribution to fetal growth, maternal health, and lactation	156:234	Perinatal care is important in mammals due to its contribution to fetal growth, maternal health, and lactation.
31572329	7	8	theme	predominant	1290:1300	arg1	Lactobacillus					1308:1320	the predominant genus Lactobacillus	1286:1320	the predominant genus Lactobacillus	1286:1320	The ratio of Bacteroidetes to Firmicutes, and the relative abundance of Prevotella significantly decreased, whilst the relative abundance of the predominant genus Lactobacillus significantly increased from LP to PO state.
31572329	10	9	theme	serum	1724:1728	arg1	metabolites					1730:1740	serum metabolites	1724:1740	serum metabolites enriched at the LP stage	1724:1765	Consistent with these changes, serum metabolites enriched at the LP stage were associated with the metabolism of amino acids and vitamins.
31572329	6	10	theme	bacteria	1019:1026	arg1	richness					1007:1014	The richness	1003:1014	The richness of bacteria in the gut of both pregnant and delivery sows	1003:1072	The richness of bacteria in the gut of both pregnant and delivery sows significantly decreased, whilst the β-diversity dramatically expanded.
31572329	4	11	theme	ideal	580:584	arg1	model					597:601	an ideal biomedical model	577:601	an ideal biomedical model for studying the interactions of the gut microbiota with host metabolism, due to the ease of controlling feeding conditions	577:725	Furthermore, pigs are an ideal biomedical model for studying the interactions of the gut microbiota with host metabolism, due to the ease of controlling feeding conditions.
31572329	4	11	theme	ideal	580:584	arg1	pigs					568:571	pigs	568:571	pigs	568:571	Furthermore, pigs are an ideal biomedical model for studying the interactions of the gut microbiota with host metabolism, due to the ease of controlling feeding conditions.
31572329	5	12	theme	validated	962:970	arg1	populations					972:982	both experimental and validated populations	940:982	both experimental and validated populations of sows (n = 107)	940:1000	Here we report dramatic remodeling of the gut microbiota and the potential functional capacity during the late stages of pregnancy (5 days before parturition, LP) to postpartum (within 6 h after delivery, PO) in both experimental and validated populations of sows (n = 107).
31572329	5	13	theme	microbiota	774:783	arg1	remodeling					752:761	dramatic remodeling	743:761	dramatic remodeling of the gut microbiota	743:783	Here we report dramatic remodeling of the gut microbiota and the potential functional capacity during the late stages of pregnancy (5 days before parturition, LP) to postpartum (within 6 h after delivery, PO) in both experimental and validated populations of sows (n = 107).
31572329	5	13	theme	microbiota	774:783	arg1	capacity					814:821	the potential functional capacity	789:821	the potential functional capacity during the late stages of pregnancy (5 days before parturition, LP) to postpartum (within 6 h after delivery, PO) in both experimental and validated populations of sows (n = 107)	789:1000	Here we report dramatic remodeling of the gut microbiota and the potential functional capacity during the late stages of pregnancy (5 days before parturition, LP) to postpartum (within 6 h after delivery, PO) in both experimental and validated populations of sows (n = 107).
31572329	7	14	theme	relative	1264:1271	arg1	abundance					1273:1281	the relative abundance	1260:1281	the relative abundance of the predominant genus Lactobacillus	1260:1320	The ratio of Bacteroidetes to Firmicutes, and the relative abundance of Prevotella significantly decreased, whilst the relative abundance of the predominant genus Lactobacillus significantly increased from LP to PO state.
31572329	9	15	theme	functional	1605:1614	arg1	capacities					1616:1625	the functional capacities	1601:1625	the functional capacities associated with carbohydrate and lipid metabolism	1601:1675	However, the abundance of the functional capacities associated with carbohydrate and lipid metabolism were increased.
31572329	4	16	theme	host	660:663	arg1	metabolism					665:674	host metabolism	660:674	host metabolism	660:674	Furthermore, pigs are an ideal biomedical model for studying the interactions of the gut microbiota with host metabolism, due to the ease of controlling feeding conditions.
31572329	13	17	theme	perinatal	2188:2196	arg1	period					2198:2203	the perinatal period	2184:2203	the perinatal period	2184:2203	The results suggest that host-microbiota interactions during the perinatal period impact host metabolism.
31572329	11	18	from	contrast	1835:1842	arg1	related					1887:1893	related	1887:1893	related	1887:1893	In contrast, metabolites enriched at the PO stage were related to lipid metabolism.
31572329	12	19	theme	gut	1979:1981	arg1	microbiota					1983:1992	the gut microbiota	1975:1992	the gut microbiota	1975:1992	We further identified that the richness and β-diversity of the gut microbiota and the abundance of Lactobacillus accounted for shifts in the levels of bile acid metabolites associated with lipid metabolism.
31572329	8	20	dep	state	1568:1572	arg1	LP					1559:1560	LP	1559:1560	LP	1559:1560	The predicted functional capacities of the gut microbiome related to amino acid metabolism, the metabolism of cofactors and vitamins, and glycan biosynthesis were significantly decreased from LP to PO state.
31572329	5	21	theme	experimental	945:956	arg1	populations					972:982	both experimental and validated populations	940:982	both experimental and validated populations of sows (n = 107)	940:1000	Here we report dramatic remodeling of the gut microbiota and the potential functional capacity during the late stages of pregnancy (5 days before parturition, LP) to postpartum (within 6 h after delivery, PO) in both experimental and validated populations of sows (n = 107).
31572329	1	22	theme	Perinatal	125:133	arg1	care					135:138	Perinatal care	125:138	Perinatal care	125:138	Perinatal care is important in mammals due to its contribution to fetal growth, maternal health, and lactation.
31572329	8	23	theme	predicted	1371:1379	arg1	capacities					1392:1401	The predicted functional capacities	1367:1401	The predicted functional capacities of the gut microbiome related to amino acid metabolism, the metabolism of cofactors and vitamins, and glycan biosynthesis	1367:1523	The predicted functional capacities of the gut microbiome related to amino acid metabolism, the metabolism of cofactors and vitamins, and glycan biosynthesis were significantly decreased from LP to PO state.
31572329	7	24	theme	Prevotella	1217:1226	arg1	ratio					1149:1153	The ratio	1145:1153	The ratio of Bacteroidetes to Firmicutes	1145:1184	The ratio of Bacteroidetes to Firmicutes, and the relative abundance of Prevotella significantly decreased, whilst the relative abundance of the predominant genus Lactobacillus significantly increased from LP to PO state.
31572329	7	24	theme	Prevotella	1217:1226	arg1	abundance					1204:1212	the relative abundance	1191:1212	the relative abundance of Prevotella	1191:1226	The ratio of Bacteroidetes to Firmicutes, and the relative abundance of Prevotella significantly decreased, whilst the relative abundance of the predominant genus Lactobacillus significantly increased from LP to PO state.
31572329	11	25	theme	PO	1873:1874	arg1	stage					1876:1880	the PO stage	1869:1880	the PO stage	1869:1880	In contrast, metabolites enriched at the PO stage were related to lipid metabolism.
31572329	1	26	from	mammals	156:162	arg1	important					143:151	important	143:151	important	143:151	Perinatal care is important in mammals due to its contribution to fetal growth, maternal health, and lactation.
31572329	5	27	theme	late	834:837	arg1	stages					839:844	the late stages	830:844	the late stages of pregnancy (5 days before parturition, LP) to postpartum (within 6 h after delivery, PO) in both experimental and validated populations of sows (n = 107)	830:1000	Here we report dramatic remodeling of the gut microbiota and the potential functional capacity during the late stages of pregnancy (5 days before parturition, LP) to postpartum (within 6 h after delivery, PO) in both experimental and validated populations of sows (n = 107).
31572329	9	28	theme	lipid	1660:1664	arg1	metabolism					1666:1675	lipid metabolism	1660:1675	lipid metabolism	1660:1675	However, the abundance of the functional capacities associated with carbohydrate and lipid metabolism were increased.
31572329	4	29	theme	microbiota	644:653	arg1	interactions					620:631	the interactions	616:631	the interactions of the gut microbiota with host metabolism, due to the ease of controlling feeding conditions	616:725	Furthermore, pigs are an ideal biomedical model for studying the interactions of the gut microbiota with host metabolism, due to the ease of controlling feeding conditions.
31572329	1	30	from	important	143:151	arg1	mammals					156:162	mammals	156:162	mammals due to its contribution to fetal growth, maternal health, and lactation	156:234	Perinatal care is important in mammals due to its contribution to fetal growth, maternal health, and lactation.
31572329	0	31	theme	Host	87:90	arg1	Changes					108:114	Host Serum Metabolic Changes	87:114	Host Serum Metabolic Changes in Sows	87:122	Dramatic Remodeling of the Gut Microbiome Around Parturition and Its Relationship With Host Serum Metabolic Changes in Sows.
31572329	12	32	dep	richness	1947:1954	arg1	the					1943:1945	the	1943:1945	the	1943:1945	We further identified that the richness and β-diversity of the gut microbiota and the abundance of Lactobacillus accounted for shifts in the levels of bile acid metabolites associated with lipid metabolism.
31572329	8	33	theme	microbiome	1414:1423	arg1	capacities					1392:1401	The predicted functional capacities	1367:1401	The predicted functional capacities of the gut microbiome related to amino acid metabolism, the metabolism of cofactors and vitamins, and glycan biosynthesis	1367:1523	The predicted functional capacities of the gut microbiome related to amino acid metabolism, the metabolism of cofactors and vitamins, and glycan biosynthesis were significantly decreased from LP to PO state.
31572329	0	34	theme	Metabolic	98:106	arg1	Changes					108:114	Host Serum Metabolic Changes	87:114	Host Serum Metabolic Changes in Sows	87:122	Dramatic Remodeling of the Gut Microbiome Around Parturition and Its Relationship With Host Serum Metabolic Changes in Sows.
31572329	5	35	theme	=	995:995	arg1	n					993:993	n = 107	993:999	n = 107	993:999	Here we report dramatic remodeling of the gut microbiota and the potential functional capacity during the late stages of pregnancy (5 days before parturition, LP) to postpartum (within 6 h after delivery, PO) in both experimental and validated populations of sows (n = 107).
31572329	5	35	theme	=	995:995	arg1	sows					987:990	sows	987:990	sows (n = 107)	987:1000	Here we report dramatic remodeling of the gut microbiota and the potential functional capacity during the late stages of pregnancy (5 days before parturition, LP) to postpartum (within 6 h after delivery, PO) in both experimental and validated populations of sows (n = 107).
31572329	0	36	theme	Dramatic	0:7	arg1	Remodeling					9:18	Dramatic Remodeling	0:18	Dramatic Remodeling of the Gut Microbiome Around Parturition	0:59	Dramatic Remodeling of the Gut Microbiome Around Parturition and Its Relationship With Host Serum Metabolic Changes in Sows.
31572329	7	37	theme	relative	1195:1202	arg1	abundance					1204:1212	the relative abundance	1191:1212	the relative abundance of Prevotella	1191:1226	The ratio of Bacteroidetes to Firmicutes, and the relative abundance of Prevotella significantly decreased, whilst the relative abundance of the predominant genus Lactobacillus significantly increased from LP to PO state.
31572329	8	38	theme	acid	1442:1445	arg1	metabolism					1447:1456	amino acid metabolism	1436:1456	amino acid metabolism	1436:1456	The predicted functional capacities of the gut microbiome related to amino acid metabolism, the metabolism of cofactors and vitamins, and glycan biosynthesis were significantly decreased from LP to PO state.
31572329	12	39	theme	bile	2067:2070	arg1	acid					2072:2075	bile acid	2067:2075	bile acid metabolites associated with lipid metabolism	2067:2120	We further identified that the richness and β-diversity of the gut microbiota and the abundance of Lactobacillus accounted for shifts in the levels of bile acid metabolites associated with lipid metabolism.
31572329	0	40	theme	Gut	27:29	arg1	Microbiome					31:40	the Gut Microbiome	23:40	the Gut Microbiome Around Parturition	23:59	Dramatic Remodeling of the Gut Microbiome Around Parturition and Its Relationship With Host Serum Metabolic Changes in Sows.
31572329	2	41	from	alterations	352:362	arg1	microbiome					375:384	the gut microbiome	367:384	the gut microbiome	367:384	Substantial changes in host hormones, metabolism, and immunity around the parturition period may be accompanied by alterations in the gut microbiome.
31572329	12	42	theme	metabolites	2077:2087	arg1	levels					2057:2062	the levels	2053:2062	the levels of bile acid metabolites associated with lipid metabolism	2053:2120	We further identified that the richness and β-diversity of the gut microbiota and the abundance of Lactobacillus accounted for shifts in the levels of bile acid metabolites associated with lipid metabolism.
31572329	3	43	from	changes	414:420	arg1	microbiome					433:442	the gut microbiome	425:442	the gut microbiome	425:442	However, to our knowledge, changes in the gut microbiome and their contribution to the shifts in host metabolism around parturition have not been investigated in pigs.
31572329	2	44	theme	parturition	311:321	arg1	period					323:328	the parturition period	307:328	the parturition period	307:328	Substantial changes in host hormones, metabolism, and immunity around the parturition period may be accompanied by alterations in the gut microbiome.
31572329	7	45	theme	Bacteroidetes	1158:1170	arg1	ratio					1149:1153	The ratio	1145:1153	The ratio of Bacteroidetes to Firmicutes	1145:1184	The ratio of Bacteroidetes to Firmicutes, and the relative abundance of Prevotella significantly decreased, whilst the relative abundance of the predominant genus Lactobacillus significantly increased from LP to PO state.
31572329	7	45	theme	Bacteroidetes	1158:1170	arg1	abundance					1204:1212	the relative abundance	1191:1212	the relative abundance of Prevotella	1191:1226	The ratio of Bacteroidetes to Firmicutes, and the relative abundance of Prevotella significantly decreased, whilst the relative abundance of the predominant genus Lactobacillus significantly increased from LP to PO state.
31572329	8	46	theme	cofactors	1477:1485	arg1	metabolism					1447:1456	amino acid metabolism	1436:1456	amino acid metabolism	1436:1456	The predicted functional capacities of the gut microbiome related to amino acid metabolism, the metabolism of cofactors and vitamins, and glycan biosynthesis were significantly decreased from LP to PO state.
31572329	8	46	theme	cofactors	1477:1485	arg1	biosynthesis					1512:1523	glycan biosynthesis	1505:1523	glycan biosynthesis	1505:1523	The predicted functional capacities of the gut microbiome related to amino acid metabolism, the metabolism of cofactors and vitamins, and glycan biosynthesis were significantly decreased from LP to PO state.
31572329	8	46	theme	cofactors	1477:1485	arg1	metabolism					1463:1472	the metabolism	1459:1472	the metabolism of cofactors and vitamins	1459:1498	The predicted functional capacities of the gut microbiome related to amino acid metabolism, the metabolism of cofactors and vitamins, and glycan biosynthesis were significantly decreased from LP to PO state.
31572329	3	47	theme	gut	429:431	arg1	microbiome					433:442	the gut microbiome	425:442	the gut microbiome	425:442	However, to our knowledge, changes in the gut microbiome and their contribution to the shifts in host metabolism around parturition have not been investigated in pigs.
31572329	13	48	theme	host-microbiota	2148:2162	arg1	interactions					2164:2175	host-microbiota interactions	2148:2175	host-microbiota interactions during the perinatal period	2148:2203	The results suggest that host-microbiota interactions during the perinatal period impact host metabolism.
31572329	8	49	theme	vitamins	1491:1498	arg1	metabolism					1447:1456	amino acid metabolism	1436:1456	amino acid metabolism	1436:1456	The predicted functional capacities of the gut microbiome related to amino acid metabolism, the metabolism of cofactors and vitamins, and glycan biosynthesis were significantly decreased from LP to PO state.
31572329	8	49	theme	vitamins	1491:1498	arg1	biosynthesis					1512:1523	glycan biosynthesis	1505:1523	glycan biosynthesis	1505:1523	The predicted functional capacities of the gut microbiome related to amino acid metabolism, the metabolism of cofactors and vitamins, and glycan biosynthesis were significantly decreased from LP to PO state.
31572329	8	49	theme	vitamins	1491:1498	arg1	metabolism					1463:1472	the metabolism	1459:1472	the metabolism of cofactors and vitamins	1459:1498	The predicted functional capacities of the gut microbiome related to amino acid metabolism, the metabolism of cofactors and vitamins, and glycan biosynthesis were significantly decreased from LP to PO state.
31572329	5	50	theme	functional	803:812	arg1	capacity					814:821	the potential functional capacity	789:821	the potential functional capacity during the late stages of pregnancy (5 days before parturition, LP) to postpartum (within 6 h after delivery, PO) in both experimental and validated populations of sows (n = 107)	789:1000	Here we report dramatic remodeling of the gut microbiota and the potential functional capacity during the late stages of pregnancy (5 days before parturition, LP) to postpartum (within 6 h after delivery, PO) in both experimental and validated populations of sows (n = 107).
31572329	11	51	from	related	1887:1893	arg1	contrast					1835:1842	contrast	1835:1842	contrast	1835:1842	In contrast, metabolites enriched at the PO stage were related to lipid metabolism.
31572329	10	52	theme	acids	1812:1816	arg1	metabolism					1792:1801	the metabolism	1788:1801	the metabolism of amino acids and vitamins	1788:1829	Consistent with these changes, serum metabolites enriched at the LP stage were associated with the metabolism of amino acids and vitamins.
31572329	14	53	theme	milk	2311:2314	arg1	production					2316:2325	milk production	2311:2325	milk production	2311:2325	These benefit the lactation of sows by providing energy from lipid metabolism for milk production.
31572329	0	54	with	Relationship	69:80	arg1	Changes					108:114	Host Serum Metabolic Changes	87:114	Host Serum Metabolic Changes in Sows	87:122	Dramatic Remodeling of the Gut Microbiome Around Parturition and Its Relationship With Host Serum Metabolic Changes in Sows.
31572329	11	55	theme	lipid	1898:1902	arg1	metabolism					1904:1913	lipid metabolism	1898:1913	lipid metabolism	1898:1913	In contrast, metabolites enriched at the PO stage were related to lipid metabolism.
31572329	12	56	from	shifts	2043:2048	arg1	levels					2057:2062	the levels	2053:2062	the levels of bile acid metabolites associated with lipid metabolism	2053:2120	We further identified that the richness and β-diversity of the gut microbiota and the abundance of Lactobacillus accounted for shifts in the levels of bile acid metabolites associated with lipid metabolism.
31572329	7	57	theme	PO	1357:1358	arg1	state					1360:1364	PO state	1357:1364	PO state	1357:1364	The ratio of Bacteroidetes to Firmicutes, and the relative abundance of Prevotella significantly decreased, whilst the relative abundance of the predominant genus Lactobacillus significantly increased from LP to PO state.
31572329	3	58	from	shifts	474:479	arg1	metabolism					489:498	host metabolism	484:498	host metabolism around parturition	484:517	However, to our knowledge, changes in the gut microbiome and their contribution to the shifts in host metabolism around parturition have not been investigated in pigs.
31572329	5	59	theme	potential	793:801	arg1	capacity					814:821	the potential functional capacity	789:821	the potential functional capacity during the late stages of pregnancy (5 days before parturition, LP) to postpartum (within 6 h after delivery, PO) in both experimental and validated populations of sows (n = 107)	789:1000	Here we report dramatic remodeling of the gut microbiota and the potential functional capacity during the late stages of pregnancy (5 days before parturition, LP) to postpartum (within 6 h after delivery, PO) in both experimental and validated populations of sows (n = 107).
31572329	14	60	from	metabolism	2296:2305	arg1	energy					2278:2283	energy	2278:2283	energy from lipid metabolism for milk production	2278:2325	These benefit the lactation of sows by providing energy from lipid metabolism for milk production.
31572329	10	61	theme	vitamins	1822:1829	arg1	metabolism					1792:1801	the metabolism	1788:1801	the metabolism of amino acids and vitamins	1788:1829	Consistent with these changes, serum metabolites enriched at the LP stage were associated with the metabolism of amino acids and vitamins.
31572329	7	62	theme	genus	1302:1306	arg1	Lactobacillus					1308:1320	the predominant genus Lactobacillus	1286:1320	the predominant genus Lactobacillus	1286:1320	The ratio of Bacteroidetes to Firmicutes, and the relative abundance of Prevotella significantly decreased, whilst the relative abundance of the predominant genus Lactobacillus significantly increased from LP to PO state.
31572329	14	63	theme	sows	2260:2263	arg1	lactation					2247:2255	the lactation	2243:2255	the lactation of sows	2243:2263	These benefit the lactation of sows by providing energy from lipid metabolism for milk production.
31572329	5	64	theme	gut	770:772	arg1	microbiota					774:783	the gut microbiota	766:783	the gut microbiota	766:783	Here we report dramatic remodeling of the gut microbiota and the potential functional capacity during the late stages of pregnancy (5 days before parturition, LP) to postpartum (within 6 h after delivery, PO) in both experimental and validated populations of sows (n = 107).
31572329	0	65	with	Remodeling	9:18	arg1	Changes					108:114	Host Serum Metabolic Changes	87:114	Host Serum Metabolic Changes in Sows	87:122	Dramatic Remodeling of the Gut Microbiome Around Parturition and Its Relationship With Host Serum Metabolic Changes in Sows.
31572329	12	66	theme	microbiota	1983:1992	arg1	richness					1947:1954	richness	1947:1954	richness	1947:1954	We further identified that the richness and β-diversity of the gut microbiota and the abundance of Lactobacillus accounted for shifts in the levels of bile acid metabolites associated with lipid metabolism.
31572329	12	66	theme	microbiota	1983:1992	arg1	abundance					2002:2010	the abundance	1998:2010	the abundance of Lactobacillus	1998:2027	We further identified that the richness and β-diversity of the gut microbiota and the abundance of Lactobacillus accounted for shifts in the levels of bile acid metabolites associated with lipid metabolism.
31572329	12	66	theme	microbiota	1983:1992	arg1	β-diversity					1960:1970	β-diversity	1960:1970	β-diversity	1960:1970	We further identified that the richness and β-diversity of the gut microbiota and the abundance of Lactobacillus accounted for shifts in the levels of bile acid metabolites associated with lipid metabolism.
31572329	1	67	theme	fetal	191:195	arg1	growth					197:202	fetal growth	191:202	fetal growth	191:202	Perinatal care is important in mammals due to its contribution to fetal growth, maternal health, and lactation.
31572329	6	68	dep	both	1042:1045	arg1	pregnant					1047:1054	pregnant	1047:1054	pregnant	1047:1054	The richness of bacteria in the gut of both pregnant and delivery sows significantly decreased, whilst the β-diversity dramatically expanded.
31572329	5	69	theme	sows	987:990	arg1	populations					972:982	both experimental and validated populations	940:982	both experimental and validated populations of sows (n = 107)	940:1000	Here we report dramatic remodeling of the gut microbiota and the potential functional capacity during the late stages of pregnancy (5 days before parturition, LP) to postpartum (within 6 h after delivery, PO) in both experimental and validated populations of sows (n = 107).
31572329	5	70	theme	pregnancy	849:857	arg1	stages					839:844	the late stages	830:844	the late stages of pregnancy (5 days before parturition, LP) to postpartum (within 6 h after delivery, PO) in both experimental and validated populations of sows (n = 107)	830:1000	Here we report dramatic remodeling of the gut microbiota and the potential functional capacity during the late stages of pregnancy (5 days before parturition, LP) to postpartum (within 6 h after delivery, PO) in both experimental and validated populations of sows (n = 107).
31572329	9	71	theme	capacities	1616:1625	arg1	abundance					1588:1596	the abundance	1584:1596	the abundance of the functional capacities associated with carbohydrate and lipid metabolism	1584:1675	However, the abundance of the functional capacities associated with carbohydrate and lipid metabolism were increased.
31572329	0	72	from	Changes	108:114	arg1	Sows					119:122	Sows	119:122	Sows	119:122	Dramatic Remodeling of the Gut Microbiome Around Parturition and Its Relationship With Host Serum Metabolic Changes in Sows.
31572329	2	73	theme	Substantial	237:247	arg1	changes					249:255	Substantial changes	237:255	Substantial changes in host hormones, metabolism, and immunity around the parturition period	237:328	Substantial changes in host hormones, metabolism, and immunity around the parturition period may be accompanied by alterations in the gut microbiome.
31572329	2	74	from	changes	249:255	arg1	metabolism					275:284	metabolism	275:284	metabolism	275:284	Substantial changes in host hormones, metabolism, and immunity around the parturition period may be accompanied by alterations in the gut microbiome.
31572329	2	74	from	changes	249:255	arg1	immunity					291:298	immunity	291:298	immunity	291:298	Substantial changes in host hormones, metabolism, and immunity around the parturition period may be accompanied by alterations in the gut microbiome.
31572329	2	74	from	changes	249:255	arg1	hormones					265:272	host hormones	260:272	host hormones	260:272	Substantial changes in host hormones, metabolism, and immunity around the parturition period may be accompanied by alterations in the gut microbiome.
31572329	8	75	theme	functional	1381:1390	arg1	capacities					1392:1401	The predicted functional capacities	1367:1401	The predicted functional capacities of the gut microbiome related to amino acid metabolism, the metabolism of cofactors and vitamins, and glycan biosynthesis	1367:1523	The predicted functional capacities of the gut microbiome related to amino acid metabolism, the metabolism of cofactors and vitamins, and glycan biosynthesis were significantly decreased from LP to PO state.
31572329	6	76	theme	sows	1069:1072	arg1	gut					1035:1037	the gut	1031:1037	the gut of both pregnant and delivery sows	1031:1072	The richness of bacteria in the gut of both pregnant and delivery sows significantly decreased, whilst the β-diversity dramatically expanded.
31572329	6	77	dep	sows	1069:1072	arg1	both					1042:1045	both	1042:1045	both	1042:1045	The richness of bacteria in the gut of both pregnant and delivery sows significantly decreased, whilst the β-diversity dramatically expanded.
31572329	6	77	dep	sows	1069:1072	arg1	delivery					1060:1067	delivery	1060:1067	delivery	1060:1067	The richness of bacteria in the gut of both pregnant and delivery sows significantly decreased, whilst the β-diversity dramatically expanded.
31572329	13	78	theme	host	2212:2215	arg1	metabolism					2217:2226	host metabolism	2212:2226	host metabolism	2212:2226	The results suggest that host-microbiota interactions during the perinatal period impact host metabolism.
31572329	8	79	theme	gut	1410:1412	arg1	microbiome					1414:1423	the gut microbiome	1406:1423	the gut microbiome related to amino acid metabolism, the metabolism of cofactors and vitamins, and glycan biosynthesis	1406:1523	The predicted functional capacities of the gut microbiome related to amino acid metabolism, the metabolism of cofactors and vitamins, and glycan biosynthesis were significantly decreased from LP to PO state.
31572329	4	80	theme	gut	640:642	arg1	microbiota					644:653	the gut microbiota	636:653	the gut microbiota	636:653	Furthermore, pigs are an ideal biomedical model for studying the interactions of the gut microbiota with host metabolism, due to the ease of controlling feeding conditions.
31572329	0	81	theme	Serum	92:96	arg1	Changes					108:114	Host Serum Metabolic Changes	87:114	Host Serum Metabolic Changes in Sows	87:122	Dramatic Remodeling of the Gut Microbiome Around Parturition and Its Relationship With Host Serum Metabolic Changes in Sows.
31572329	10	82	with	Consistent	1693:1702	arg1	changes					1715:1721	these changes	1709:1721	these changes	1709:1721	Consistent with these changes, serum metabolites enriched at the LP stage were associated with the metabolism of amino acids and vitamins.
31572329	8	83	theme	related	1425:1431	arg1	microbiome					1414:1423	the gut microbiome	1406:1423	the gut microbiome related to amino acid metabolism, the metabolism of cofactors and vitamins, and glycan biosynthesis	1406:1523	The predicted functional capacities of the gut microbiome related to amino acid metabolism, the metabolism of cofactors and vitamins, and glycan biosynthesis were significantly decreased from LP to PO state.
31572329	5	84	dep	days	862:865	arg1	LP					887:888	LP	887:888	LP	887:888	Here we report dramatic remodeling of the gut microbiota and the potential functional capacity during the late stages of pregnancy (5 days before parturition, LP) to postpartum (within 6 h after delivery, PO) in both experimental and validated populations of sows (n = 107).
31572329	8	85	theme	amino	1436:1440	arg1	metabolism					1447:1456	amino acid metabolism	1436:1456	amino acid metabolism	1436:1456	The predicted functional capacities of the gut microbiome related to amino acid metabolism, the metabolism of cofactors and vitamins, and glycan biosynthesis were significantly decreased from LP to PO state.
31572329	4	86	theme	feeding	708:714	arg1	conditions					716:725	feeding conditions	708:725	feeding conditions	708:725	Furthermore, pigs are an ideal biomedical model for studying the interactions of the gut microbiota with host metabolism, due to the ease of controlling feeding conditions.
31572329	5	87	theme	dramatic	743:750	arg1	remodeling					752:761	dramatic remodeling	743:761	dramatic remodeling of the gut microbiota	743:783	Here we report dramatic remodeling of the gut microbiota and the potential functional capacity during the late stages of pregnancy (5 days before parturition, LP) to postpartum (within 6 h after delivery, PO) in both experimental and validated populations of sows (n = 107).
31572329	4	88	theme	due	677:679	arg1	metabolism					665:674	host metabolism	660:674	host metabolism	660:674	Furthermore, pigs are an ideal biomedical model for studying the interactions of the gut microbiota with host metabolism, due to the ease of controlling feeding conditions.
31572329	4	89	with	interactions	620:631	arg1	metabolism					665:674	host metabolism	660:674	host metabolism	660:674	Furthermore, pigs are an ideal biomedical model for studying the interactions of the gut microbiota with host metabolism, due to the ease of controlling feeding conditions.
31572329	12	90	theme	lipid	2105:2109	arg1	metabolism					2111:2120	lipid metabolism	2105:2120	lipid metabolism	2105:2120	We further identified that the richness and β-diversity of the gut microbiota and the abundance of Lactobacillus accounted for shifts in the levels of bile acid metabolites associated with lipid metabolism.
31572329	0	91	theme	Microbiome	31:40	arg1	Relationship					69:80	Its Relationship	65:80	Its Relationship With Host Serum Metabolic Changes in Sows	65:122	Dramatic Remodeling of the Gut Microbiome Around Parturition and Its Relationship With Host Serum Metabolic Changes in Sows.
31572329	0	91	theme	Microbiome	31:40	arg1	Remodeling					9:18	Dramatic Remodeling	0:18	Dramatic Remodeling of the Gut Microbiome Around Parturition	0:59	Dramatic Remodeling of the Gut Microbiome Around Parturition and Its Relationship With Host Serum Metabolic Changes in Sows.
31572329	10	92	theme	LP	1758:1759	arg1	stage					1761:1765	the LP stage	1754:1765	the LP stage	1754:1765	Consistent with these changes, serum metabolites enriched at the LP stage were associated with the metabolism of amino acids and vitamins.
31572329	2	93	theme	gut	371:373	arg1	microbiome					375:384	the gut microbiome	367:384	the gut microbiome	367:384	Substantial changes in host hormones, metabolism, and immunity around the parturition period may be accompanied by alterations in the gut microbiome.
31572329	12	94	theme	acid	2072:2075	arg1	metabolites					2077:2087	bile acid metabolites	2067:2087	bile acid metabolites associated with lipid metabolism	2067:2120	We further identified that the richness and β-diversity of the gut microbiota and the abundance of Lactobacillus accounted for shifts in the levels of bile acid metabolites associated with lipid metabolism.
31572329	6	95	from	richness	1007:1014	arg1	gut					1035:1037	the gut	1031:1037	the gut of both pregnant and delivery sows	1031:1072	The richness of bacteria in the gut of both pregnant and delivery sows significantly decreased, whilst the β-diversity dramatically expanded.
31572329	7	96	dep	state	1360:1364	arg1	LP					1351:1352	LP	1351:1352	LP	1351:1352	The ratio of Bacteroidetes to Firmicutes, and the relative abundance of Prevotella significantly decreased, whilst the relative abundance of the predominant genus Lactobacillus significantly increased from LP to PO state.
31572329	8	97	theme	PO	1565:1566	arg1	state					1568:1572	PO state	1565:1572	PO state	1565:1572	The predicted functional capacities of the gut microbiome related to amino acid metabolism, the metabolism of cofactors and vitamins, and glycan biosynthesis were significantly decreased from LP to PO state.
31572329	1	98	theme	maternal	205:212	arg1	health					214:219	maternal health	205:219	maternal health	205:219	Perinatal care is important in mammals due to its contribution to fetal growth, maternal health, and lactation.
31572329	4	99	theme	biomedical	586:595	arg1	model					597:601	an ideal biomedical model	577:601	an ideal biomedical model for studying the interactions of the gut microbiota with host metabolism, due to the ease of controlling feeding conditions	577:725	Furthermore, pigs are an ideal biomedical model for studying the interactions of the gut microbiota with host metabolism, due to the ease of controlling feeding conditions.
31572329	4	99	theme	biomedical	586:595	arg1	pigs					568:571	pigs	568:571	pigs	568:571	Furthermore, pigs are an ideal biomedical model for studying the interactions of the gut microbiota with host metabolism, due to the ease of controlling feeding conditions.
31572329	8	100	theme	glycan	1505:1510	arg1	biosynthesis					1512:1523	glycan biosynthesis	1505:1523	glycan biosynthesis	1505:1523	The predicted functional capacities of the gut microbiome related to amino acid metabolism, the metabolism of cofactors and vitamins, and glycan biosynthesis were significantly decreased from LP to PO state.
31572329	5	101	from	postpartum	894:903	arg1	populations					972:982	both experimental and validated populations	940:982	both experimental and validated populations of sows (n = 107)	940:1000	Here we report dramatic remodeling of the gut microbiota and the potential functional capacity during the late stages of pregnancy (5 days before parturition, LP) to postpartum (within 6 h after delivery, PO) in both experimental and validated populations of sows (n = 107).
30657762	7	0	theme	BMN	1458:1460	arg1	uptake					1466:1471	BMN 250 uptake	1458:1471	BMN 250 uptake in Sanfilippo B patient fibroblasts	1458:1507	Similar to neurons and astrocytes, BMN 250 uptake in Sanfilippo B patient fibroblasts is predominantly CI-MPR-mediated, resulting in augmentation of NAGLU activity with doses of enzyme that fall well below the Kuptake (5 nM), which are sufficient to prevent HS accumulation.
30657762	2	1	theme	-tagged	612:618	arg1	molecule					626:633	an insulin-like growth factor 2 (IGF2)-tagged NAGLU molecule	574:633	an insulin-like growth factor 2 (IGF2)-tagged NAGLU molecule (BMN 250; tralesinidase alfa) that binds avidly to the IGF2 / cation-independent mannose 6-phosphate receptor (CI-MPR) for glycosylation independent lysosomal targeting	574:802	Because NAGLU expressed in Chinese hamster ovary cells is not mannose-6-phosphorylated, we developed an insulin-like growth factor 2 (IGF2)-tagged NAGLU molecule (BMN 250; tralesinidase alfa) that binds avidly to the IGF2 / cation-independent mannose 6-phosphate receptor (CI-MPR) for glycosylation independent lysosomal targeting.
30657762	2	1	theme	-tagged	612:618	arg1	alfa					659:662	BMN 250; tralesinidase alfa	636:662	BMN 250; tralesinidase alfa	636:662	Because NAGLU expressed in Chinese hamster ovary cells is not mannose-6-phosphorylated, we developed an insulin-like growth factor 2 (IGF2)-tagged NAGLU molecule (BMN 250; tralesinidase alfa) that binds avidly to the IGF2 / cation-independent mannose 6-phosphate receptor (CI-MPR) for glycosylation independent lysosomal targeting.
30657762	0	2	theme	B	167:167	arg1	pathogenesis					177:188	Sanfilippo syndrome B disease pathogenesis	147:188	Sanfilippo syndrome B disease pathogenesis	147:188	BMN 250, a fusion of lysosomal alpha-N-acetylglucosaminidase with IGF2, exhibits different patterns of cellular uptake into critical cell types of Sanfilippo syndrome B disease pathogenesis.
30657762	1	3	theme	type	255:258	arg1	IIIB					260:263	Mucopolysaccharidosis type IIIB	233:263	Sanfilippo B; Mucopolysaccharidosis type IIIB	219:263	Sanfilippo syndrome type B (Sanfilippo B; Mucopolysaccharidosis type IIIB) occurs due to genetic deficiency of lysosomal alpha-N-acetylglucosaminidase (NAGLU) and subsequent lysosomal accumulation of heparan sulfate (HS), which coincides with devastating neurodegenerative disease.
30657762	0	4	theme	pathogenesis	177:188	arg1	types					138:142	critical cell types	124:142	critical cell types of Sanfilippo syndrome B disease pathogenesis	124:188	BMN 250, a fusion of lysosomal alpha-N-acetylglucosaminidase with IGF2, exhibits different patterns of cellular uptake into critical cell types of Sanfilippo syndrome B disease pathogenesis.
30657762	1	5	theme	neurodegenerative	446:462	arg1	disease					464:470	devastating neurodegenerative disease	434:470	devastating neurodegenerative disease	434:470	Sanfilippo syndrome type B (Sanfilippo B; Mucopolysaccharidosis type IIIB) occurs due to genetic deficiency of lysosomal alpha-N-acetylglucosaminidase (NAGLU) and subsequent lysosomal accumulation of heparan sulfate (HS), which coincides with devastating neurodegenerative disease.
30657762	6	6	from	uptake	1266:1271	arg1	concentration					1226:1238	the concentration	1222:1238	the concentration of enzyme at half-maximal uptake	1222:1271	Kuptake, defined as the concentration of enzyme at half-maximal uptake, is 5 nM and 3 nM in neurons and astrocytes, with a maximal uptake capacity (Vmax) corresponding to 764 nmol/hr/mg and 5380 nmol/hr/mg, respectively.
30657762	2	7	theme	BMN	636:638	arg1	molecule					626:633	an insulin-like growth factor 2 (IGF2)-tagged NAGLU molecule	574:633	an insulin-like growth factor 2 (IGF2)-tagged NAGLU molecule (BMN 250; tralesinidase alfa) that binds avidly to the IGF2 / cation-independent mannose 6-phosphate receptor (CI-MPR) for glycosylation independent lysosomal targeting	574:802	Because NAGLU expressed in Chinese hamster ovary cells is not mannose-6-phosphorylated, we developed an insulin-like growth factor 2 (IGF2)-tagged NAGLU molecule (BMN 250; tralesinidase alfa) that binds avidly to the IGF2 / cation-independent mannose 6-phosphate receptor (CI-MPR) for glycosylation independent lysosomal targeting.
30657762	2	7	theme	BMN	636:638	arg1	alfa					659:662	BMN 250; tralesinidase alfa	636:662	BMN 250; tralesinidase alfa	636:662	Because NAGLU expressed in Chinese hamster ovary cells is not mannose-6-phosphorylated, we developed an insulin-like growth factor 2 (IGF2)-tagged NAGLU molecule (BMN 250; tralesinidase alfa) that binds avidly to the IGF2 / cation-independent mannose 6-phosphate receptor (CI-MPR) for glycosylation independent lysosomal targeting.
30657762	8	8	from	uptake	1711:1716	arg1	fibroblasts					1802:1812	fibroblasts	1802:1812	fibroblasts	1802:1812	In contrast, uptake of the untagged recombinant human NAGLU (rhNAGLU) enzyme in neurons, astrocytes and fibroblasts is negligible at the same doses tested.
30657762	8	8	from	uptake	1711:1716	arg1	astrocytes					1787:1796	astrocytes	1787:1796	astrocytes	1787:1796	In contrast, uptake of the untagged recombinant human NAGLU (rhNAGLU) enzyme in neurons, astrocytes and fibroblasts is negligible at the same doses tested.
30657762	8	8	from	uptake	1711:1716	arg1	neurons					1778:1784	neurons	1778:1784	neurons	1778:1784	In contrast, uptake of the untagged recombinant human NAGLU (rhNAGLU) enzyme in neurons, astrocytes and fibroblasts is negligible at the same doses tested.
30657762	2	9	dep	molecule	626:633	arg1	CI-MPR					746:751	CI-MPR	746:751	CI-MPR	746:751	Because NAGLU expressed in Chinese hamster ovary cells is not mannose-6-phosphorylated, we developed an insulin-like growth factor 2 (IGF2)-tagged NAGLU molecule (BMN 250; tralesinidase alfa) that binds avidly to the IGF2 / cation-independent mannose 6-phosphate receptor (CI-MPR) for glycosylation independent lysosomal targeting.
30657762	2	9	dep	molecule	626:633	arg1	receptor					736:743	cation-independent mannose 6-phosphate receptor	697:743	an insulin-like growth factor 2 (IGF2)-tagged NAGLU molecule (BMN 250; tralesinidase alfa) that binds avidly to the IGF2 / cation-independent mannose 6-phosphate receptor (CI-MPR) for glycosylation independent lysosomal targeting	574:802	Because NAGLU expressed in Chinese hamster ovary cells is not mannose-6-phosphorylated, we developed an insulin-like growth factor 2 (IGF2)-tagged NAGLU molecule (BMN 250; tralesinidase alfa) that binds avidly to the IGF2 / cation-independent mannose 6-phosphate receptor (CI-MPR) for glycosylation independent lysosomal targeting.
30657762	8	10	from	contrast	1701:1708	arg1	negligible					1817:1826	negligible	1817:1826	negligible	1817:1826	In contrast, uptake of the untagged recombinant human NAGLU (rhNAGLU) enzyme in neurons, astrocytes and fibroblasts is negligible at the same doses tested.
30657762	6	11	with	nM	1288:1289	arg1	Vmax					1350:1353	Vmax	1350:1353	Vmax	1350:1353	Kuptake, defined as the concentration of enzyme at half-maximal uptake, is 5 nM and 3 nM in neurons and astrocytes, with a maximal uptake capacity (Vmax) corresponding to 764 nmol/hr/mg and 5380 nmol/hr/mg, respectively.
30657762	6	11	with	nM	1288:1289	arg1	capacity					1340:1347	a maximal uptake capacity	1323:1347	a maximal uptake capacity (Vmax) corresponding to 764 nmol/hr/mg and 5380 nmol/hr/mg, respectively	1323:1420	Kuptake, defined as the concentration of enzyme at half-maximal uptake, is 5 nM and 3 nM in neurons and astrocytes, with a maximal uptake capacity (Vmax) corresponding to 764 nmol/hr/mg and 5380 nmol/hr/mg, respectively.
30657762	4	12	theme	cellular	933:940	arg1	mechanisms					949:958	two cellular uptake mechanisms	929:958	two cellular uptake mechanisms by which BMN 250 is targeted to lysosomes	929:1000	Here we distinguish two cellular uptake mechanisms by which BMN 250 is targeted to lysosomes.
30657762	2	13	theme	lysosomal	784:792	arg1	targeting					794:802	glycosylation independent lysosomal targeting	758:802	glycosylation independent lysosomal targeting	758:802	Because NAGLU expressed in Chinese hamster ovary cells is not mannose-6-phosphorylated, we developed an insulin-like growth factor 2 (IGF2)-tagged NAGLU molecule (BMN 250; tralesinidase alfa) that binds avidly to the IGF2 / cation-independent mannose 6-phosphate receptor (CI-MPR) for glycosylation independent lysosomal targeting.
30657762	8	14	theme	same	1835:1838	arg1	doses					1840:1844	the same doses	1831:1844	the same doses tested	1831:1851	In contrast, uptake of the untagged recombinant human NAGLU (rhNAGLU) enzyme in neurons, astrocytes and fibroblasts is negligible at the same doses tested.
30657762	5	15	theme	uptake	1075:1080	arg1	majority					1056:1063	the majority	1052:1063	the majority of BMN250 uptake over 24 hours	1052:1094	In normal rodent-derived neurons and astrocytes, the majority of BMN250 uptake over 24 hours reaches saturation, which can be competitively inhibited with IGF2, suggestive of CI-MPR-mediated uptake.
30657762	10	16	theme	tag	2233:2235	arg1	moiety					2237:2242	its IGF2 tag moiety	2224:2242	its IGF2 tag moiety	2224:2242	These results suggest that while receptor-independent mechanisms exist for lysosomal targeting of rhNAGLU in microglia, BMN 250, by its IGF2 tag moiety, confers increased CI-MPR-mediated lysosomal targeting to neurons and astrocytes, two additional critical cell types of Sanfilippo B disease pathogenesis.
30657762	6	17	theme	enzyme	1243:1248	arg1	concentration					1226:1238	the concentration	1222:1238	the concentration of enzyme at half-maximal uptake	1222:1271	Kuptake, defined as the concentration of enzyme at half-maximal uptake, is 5 nM and 3 nM in neurons and astrocytes, with a maximal uptake capacity (Vmax) corresponding to 764 nmol/hr/mg and 5380 nmol/hr/mg, respectively.
30657762	2	18	theme	glycosylation	758:770	arg1	targeting					794:802	glycosylation independent lysosomal targeting	758:802	glycosylation independent lysosomal targeting	758:802	Because NAGLU expressed in Chinese hamster ovary cells is not mannose-6-phosphorylated, we developed an insulin-like growth factor 2 (IGF2)-tagged NAGLU molecule (BMN 250; tralesinidase alfa) that binds avidly to the IGF2 / cation-independent mannose 6-phosphate receptor (CI-MPR) for glycosylation independent lysosomal targeting.
30657762	0	19	theme	critical	124:131	arg1	types					138:142	critical cell types	124:142	critical cell types of Sanfilippo syndrome B disease pathogenesis	124:188	BMN 250, a fusion of lysosomal alpha-N-acetylglucosaminidase with IGF2, exhibits different patterns of cellular uptake into critical cell types of Sanfilippo syndrome B disease pathogenesis.
30657762	10	20	theme	IGF2	2228:2231	arg1	moiety					2237:2242	its IGF2 tag moiety	2224:2242	its IGF2 tag moiety	2224:2242	These results suggest that while receptor-independent mechanisms exist for lysosomal targeting of rhNAGLU in microglia, BMN 250, by its IGF2 tag moiety, confers increased CI-MPR-mediated lysosomal targeting to neurons and astrocytes, two additional critical cell types of Sanfilippo B disease pathogenesis.
30657762	2	21	dep	-tagged	612:618	arg1	factor					597:602	insulin-like growth factor 2	577:604	insulin-like growth factor 2	577:604	Because NAGLU expressed in Chinese hamster ovary cells is not mannose-6-phosphorylated, we developed an insulin-like growth factor 2 (IGF2)-tagged NAGLU molecule (BMN 250; tralesinidase alfa) that binds avidly to the IGF2 / cation-independent mannose 6-phosphate receptor (CI-MPR) for glycosylation independent lysosomal targeting.
30657762	10	22	theme	lysosomal	2279:2287	arg1	targeting					2289:2297	increased CI-MPR-mediated lysosomal targeting	2253:2297	increased CI-MPR-mediated lysosomal targeting to neurons and astrocytes, two additional critical cell types of Sanfilippo B disease pathogenesis	2253:2396	These results suggest that while receptor-independent mechanisms exist for lysosomal targeting of rhNAGLU in microglia, BMN 250, by its IGF2 tag moiety, confers increased CI-MPR-mediated lysosomal targeting to neurons and astrocytes, two additional critical cell types of Sanfilippo B disease pathogenesis.
30657762	10	23	theme	increased	2253:2261	arg1	targeting					2289:2297	increased CI-MPR-mediated lysosomal targeting	2253:2297	increased CI-MPR-mediated lysosomal targeting to neurons and astrocytes, two additional critical cell types of Sanfilippo B disease pathogenesis	2253:2396	These results suggest that while receptor-independent mechanisms exist for lysosomal targeting of rhNAGLU in microglia, BMN 250, by its IGF2 tag moiety, confers increased CI-MPR-mediated lysosomal targeting to neurons and astrocytes, two additional critical cell types of Sanfilippo B disease pathogenesis.
30657762	9	24	with	competition	1935:1945	arg1	IGF2					1959:1962	excess IGF2	1952:1962	excess IGF2	1952:1962	In microglia, receptor-independent uptake, defined as enzyme uptake resistant to competition with excess IGF2, results in appreciable lysosomal delivery of BMN 250 and rhNAGLU (Vmax = 12,336 nmol/hr/mg and 5469 nmol/hr/mg, respectively).
30657762	3	25	theme	enzyme	864:869	arg1	BMN					805:807	BMN 250	805:811	BMN 250	805:811	BMN 250 is currently being developed as an investigational enzyme replacement therapy for Sanfilippo B.
30657762	3	25	theme	enzyme	864:869	arg1	therapy					883:889	an investigational enzyme replacement therapy	845:889	an investigational enzyme replacement therapy for Sanfilippo B	845:906	BMN 250 is currently being developed as an investigational enzyme replacement therapy for Sanfilippo B.
30657762	10	26	theme	lysosomal	2167:2175	arg1	targeting					2177:2185	lysosomal targeting	2167:2185	lysosomal targeting of rhNAGLU in microglia	2167:2209	These results suggest that while receptor-independent mechanisms exist for lysosomal targeting of rhNAGLU in microglia, BMN 250, by its IGF2 tag moiety, confers increased CI-MPR-mediated lysosomal targeting to neurons and astrocytes, two additional critical cell types of Sanfilippo B disease pathogenesis.
30657762	2	27	theme	growth	590:595	arg1	factor					597:602	insulin-like growth factor 2	577:604	insulin-like growth factor 2	577:604	Because NAGLU expressed in Chinese hamster ovary cells is not mannose-6-phosphorylated, we developed an insulin-like growth factor 2 (IGF2)-tagged NAGLU molecule (BMN 250; tralesinidase alfa) that binds avidly to the IGF2 / cation-independent mannose 6-phosphate receptor (CI-MPR) for glycosylation independent lysosomal targeting.
30657762	0	28	theme	Sanfilippo	147:156	arg1	syndrome					158:165	Sanfilippo syndrome	147:165	Sanfilippo syndrome B disease pathogenesis	147:188	BMN 250, a fusion of lysosomal alpha-N-acetylglucosaminidase with IGF2, exhibits different patterns of cellular uptake into critical cell types of Sanfilippo syndrome B disease pathogenesis.
30657762	7	29	theme	NAGLU	1572:1576	arg1	activity					1578:1585	NAGLU activity	1572:1585	NAGLU activity	1572:1585	Similar to neurons and astrocytes, BMN 250 uptake in Sanfilippo B patient fibroblasts is predominantly CI-MPR-mediated, resulting in augmentation of NAGLU activity with doses of enzyme that fall well below the Kuptake (5 nM), which are sufficient to prevent HS accumulation.
30657762	2	30	theme	ovary	516:520	arg1	cells					522:526	Chinese hamster ovary cells	500:526	Chinese hamster ovary cells	500:526	Because NAGLU expressed in Chinese hamster ovary cells is not mannose-6-phosphorylated, we developed an insulin-like growth factor 2 (IGF2)-tagged NAGLU molecule (BMN 250; tralesinidase alfa) that binds avidly to the IGF2 / cation-independent mannose 6-phosphate receptor (CI-MPR) for glycosylation independent lysosomal targeting.
30657762	2	31	theme	6-phosphate	724:734	arg1	CI-MPR					746:751	CI-MPR	746:751	CI-MPR	746:751	Because NAGLU expressed in Chinese hamster ovary cells is not mannose-6-phosphorylated, we developed an insulin-like growth factor 2 (IGF2)-tagged NAGLU molecule (BMN 250; tralesinidase alfa) that binds avidly to the IGF2 / cation-independent mannose 6-phosphate receptor (CI-MPR) for glycosylation independent lysosomal targeting.
30657762	2	31	theme	6-phosphate	724:734	arg1	receptor					736:743	cation-independent mannose 6-phosphate receptor	697:743	an insulin-like growth factor 2 (IGF2)-tagged NAGLU molecule (BMN 250; tralesinidase alfa) that binds avidly to the IGF2 / cation-independent mannose 6-phosphate receptor (CI-MPR) for glycosylation independent lysosomal targeting	574:802	Because NAGLU expressed in Chinese hamster ovary cells is not mannose-6-phosphorylated, we developed an insulin-like growth factor 2 (IGF2)-tagged NAGLU molecule (BMN 250; tralesinidase alfa) that binds avidly to the IGF2 / cation-independent mannose 6-phosphate receptor (CI-MPR) for glycosylation independent lysosomal targeting.
30657762	10	32	from	targeting	2177:2185	arg1	microglia					2201:2209	microglia	2201:2209	microglia	2201:2209	These results suggest that while receptor-independent mechanisms exist for lysosomal targeting of rhNAGLU in microglia, BMN 250, by its IGF2 tag moiety, confers increased CI-MPR-mediated lysosomal targeting to neurons and astrocytes, two additional critical cell types of Sanfilippo B disease pathogenesis.
30657762	8	33	theme	recombinant	1734:1744	arg1	enzyme					1768:1773	the untagged recombinant human NAGLU (rhNAGLU) enzyme	1721:1773	the untagged recombinant human NAGLU (rhNAGLU) enzyme	1721:1773	In contrast, uptake of the untagged recombinant human NAGLU (rhNAGLU) enzyme in neurons, astrocytes and fibroblasts is negligible at the same doses tested.
30657762	3	34	theme	Sanfilippo	895:904	arg1	B					906:906	Sanfilippo B	895:906	Sanfilippo B	895:906	BMN 250 is currently being developed as an investigational enzyme replacement therapy for Sanfilippo B.
30657762	2	35	theme	Chinese	500:506	arg1	cells					522:526	Chinese hamster ovary cells	500:526	Chinese hamster ovary cells	500:526	Because NAGLU expressed in Chinese hamster ovary cells is not mannose-6-phosphorylated, we developed an insulin-like growth factor 2 (IGF2)-tagged NAGLU molecule (BMN 250; tralesinidase alfa) that binds avidly to the IGF2 / cation-independent mannose 6-phosphate receptor (CI-MPR) for glycosylation independent lysosomal targeting.
30657762	2	36	theme	cation-independent	697:714	arg1	CI-MPR					746:751	CI-MPR	746:751	CI-MPR	746:751	Because NAGLU expressed in Chinese hamster ovary cells is not mannose-6-phosphorylated, we developed an insulin-like growth factor 2 (IGF2)-tagged NAGLU molecule (BMN 250; tralesinidase alfa) that binds avidly to the IGF2 / cation-independent mannose 6-phosphate receptor (CI-MPR) for glycosylation independent lysosomal targeting.
30657762	2	36	theme	cation-independent	697:714	arg1	receptor					736:743	cation-independent mannose 6-phosphate receptor	697:743	an insulin-like growth factor 2 (IGF2)-tagged NAGLU molecule (BMN 250; tralesinidase alfa) that binds avidly to the IGF2 / cation-independent mannose 6-phosphate receptor (CI-MPR) for glycosylation independent lysosomal targeting	574:802	Because NAGLU expressed in Chinese hamster ovary cells is not mannose-6-phosphorylated, we developed an insulin-like growth factor 2 (IGF2)-tagged NAGLU molecule (BMN 250; tralesinidase alfa) that binds avidly to the IGF2 / cation-independent mannose 6-phosphate receptor (CI-MPR) for glycosylation independent lysosomal targeting.
30657762	10	37	from	rhNAGLU	2190:2196	arg1	microglia					2201:2209	microglia	2201:2209	microglia	2201:2209	These results suggest that while receptor-independent mechanisms exist for lysosomal targeting of rhNAGLU in microglia, BMN 250, by its IGF2 tag moiety, confers increased CI-MPR-mediated lysosomal targeting to neurons and astrocytes, two additional critical cell types of Sanfilippo B disease pathogenesis.
30657762	8	38	theme	NAGLU	1752:1756	arg1	enzyme					1768:1773	the untagged recombinant human NAGLU (rhNAGLU) enzyme	1721:1773	the untagged recombinant human NAGLU (rhNAGLU) enzyme	1721:1773	In contrast, uptake of the untagged recombinant human NAGLU (rhNAGLU) enzyme in neurons, astrocytes and fibroblasts is negligible at the same doses tested.
30657762	10	39	theme	additional	2330:2339	arg1	types					2355:2359	two additional critical cell types	2326:2359	two additional critical cell types of Sanfilippo B disease pathogenesis	2326:2396	These results suggest that while receptor-independent mechanisms exist for lysosomal targeting of rhNAGLU in microglia, BMN 250, by its IGF2 tag moiety, confers increased CI-MPR-mediated lysosomal targeting to neurons and astrocytes, two additional critical cell types of Sanfilippo B disease pathogenesis.
30657762	10	39	theme	additional	2330:2339	arg1	neurons					2302:2308	neurons	2302:2308	neurons	2302:2308	These results suggest that while receptor-independent mechanisms exist for lysosomal targeting of rhNAGLU in microglia, BMN 250, by its IGF2 tag moiety, confers increased CI-MPR-mediated lysosomal targeting to neurons and astrocytes, two additional critical cell types of Sanfilippo B disease pathogenesis.
30657762	2	40	theme	tralesinidase	645:657	arg1	molecule					626:633	an insulin-like growth factor 2 (IGF2)-tagged NAGLU molecule	574:633	an insulin-like growth factor 2 (IGF2)-tagged NAGLU molecule (BMN 250; tralesinidase alfa) that binds avidly to the IGF2 / cation-independent mannose 6-phosphate receptor (CI-MPR) for glycosylation independent lysosomal targeting	574:802	Because NAGLU expressed in Chinese hamster ovary cells is not mannose-6-phosphorylated, we developed an insulin-like growth factor 2 (IGF2)-tagged NAGLU molecule (BMN 250; tralesinidase alfa) that binds avidly to the IGF2 / cation-independent mannose 6-phosphate receptor (CI-MPR) for glycosylation independent lysosomal targeting.
30657762	2	40	theme	tralesinidase	645:657	arg1	alfa					659:662	BMN 250; tralesinidase alfa	636:662	BMN 250; tralesinidase alfa	636:662	Because NAGLU expressed in Chinese hamster ovary cells is not mannose-6-phosphorylated, we developed an insulin-like growth factor 2 (IGF2)-tagged NAGLU molecule (BMN 250; tralesinidase alfa) that binds avidly to the IGF2 / cation-independent mannose 6-phosphate receptor (CI-MPR) for glycosylation independent lysosomal targeting.
30657762	0	41	theme	different	81:89	arg1	patterns					91:98	different patterns	81:98	different patterns of cellular uptake into critical cell types of Sanfilippo syndrome B disease pathogenesis	81:188	BMN 250, a fusion of lysosomal alpha-N-acetylglucosaminidase with IGF2, exhibits different patterns of cellular uptake into critical cell types of Sanfilippo syndrome B disease pathogenesis.
30657762	1	42	theme	subsequent	354:363	arg1	accumulation					375:386	subsequent lysosomal accumulation	354:386	subsequent lysosomal accumulation of heparan sulfate (HS)	354:410	Sanfilippo syndrome type B (Sanfilippo B; Mucopolysaccharidosis type IIIB) occurs due to genetic deficiency of lysosomal alpha-N-acetylglucosaminidase (NAGLU) and subsequent lysosomal accumulation of heparan sulfate (HS), which coincides with devastating neurodegenerative disease.
30657762	8	43	theme	rhNAGLU	1759:1765	arg1	enzyme					1768:1773	the untagged recombinant human NAGLU (rhNAGLU) enzyme	1721:1773	the untagged recombinant human NAGLU (rhNAGLU) enzyme	1721:1773	In contrast, uptake of the untagged recombinant human NAGLU (rhNAGLU) enzyme in neurons, astrocytes and fibroblasts is negligible at the same doses tested.
30657762	0	44	theme	alpha-N-acetylglucosaminidase	31:59	arg1	BMN					0:2	BMN 250	0:6	BMN 250	0:6	BMN 250, a fusion of lysosomal alpha-N-acetylglucosaminidase with IGF2, exhibits different patterns of cellular uptake into critical cell types of Sanfilippo syndrome B disease pathogenesis.
30657762	0	44	theme	alpha-N-acetylglucosaminidase	31:59	arg1	fusion					11:16	a fusion	9:16	a fusion of lysosomal alpha-N-acetylglucosaminidase with IGF2	9:69	BMN 250, a fusion of lysosomal alpha-N-acetylglucosaminidase with IGF2, exhibits different patterns of cellular uptake into critical cell types of Sanfilippo syndrome B disease pathogenesis.
30657762	10	45	theme	receptor-independent	2125:2144	arg1	mechanisms					2146:2155	receptor-independent mechanisms	2125:2155	receptor-independent mechanisms	2125:2155	These results suggest that while receptor-independent mechanisms exist for lysosomal targeting of rhNAGLU in microglia, BMN 250, by its IGF2 tag moiety, confers increased CI-MPR-mediated lysosomal targeting to neurons and astrocytes, two additional critical cell types of Sanfilippo B disease pathogenesis.
30657762	1	46	theme	accumulation	375:386	arg1	deficiency					288:297	genetic deficiency	280:297	genetic deficiency	280:297	Sanfilippo syndrome type B (Sanfilippo B; Mucopolysaccharidosis type IIIB) occurs due to genetic deficiency of lysosomal alpha-N-acetylglucosaminidase (NAGLU) and subsequent lysosomal accumulation of heparan sulfate (HS), which coincides with devastating neurodegenerative disease.
30657762	8	47	theme	enzyme	1768:1773	arg1	uptake					1711:1716	uptake	1711:1716	uptake of the untagged recombinant human NAGLU (rhNAGLU) enzyme in neurons, astrocytes and fibroblasts	1711:1812	In contrast, uptake of the untagged recombinant human NAGLU (rhNAGLU) enzyme in neurons, astrocytes and fibroblasts is negligible at the same doses tested.
30657762	10	48	theme	cell	2350:2353	arg1	types					2355:2359	two additional critical cell types	2326:2359	two additional critical cell types of Sanfilippo B disease pathogenesis	2326:2396	These results suggest that while receptor-independent mechanisms exist for lysosomal targeting of rhNAGLU in microglia, BMN 250, by its IGF2 tag moiety, confers increased CI-MPR-mediated lysosomal targeting to neurons and astrocytes, two additional critical cell types of Sanfilippo B disease pathogenesis.
30657762	10	48	theme	cell	2350:2353	arg1	neurons					2302:2308	neurons	2302:2308	neurons	2302:2308	These results suggest that while receptor-independent mechanisms exist for lysosomal targeting of rhNAGLU in microglia, BMN 250, by its IGF2 tag moiety, confers increased CI-MPR-mediated lysosomal targeting to neurons and astrocytes, two additional critical cell types of Sanfilippo B disease pathogenesis.
30657762	9	49	theme	appreciable	1976:1986	arg1	delivery					1998:2005	appreciable lysosomal delivery	1976:2005	appreciable lysosomal delivery of BMN 250	1976:2016	In microglia, receptor-independent uptake, defined as enzyme uptake resistant to competition with excess IGF2, results in appreciable lysosomal delivery of BMN 250 and rhNAGLU (Vmax = 12,336 nmol/hr/mg and 5469 nmol/hr/mg, respectively).
30657762	5	50	theme	uptake	1194:1199	arg1	suggestive					1164:1173	suggestive	1164:1173	suggestive	1164:1173	In normal rodent-derived neurons and astrocytes, the majority of BMN250 uptake over 24 hours reaches saturation, which can be competitively inhibited with IGF2, suggestive of CI-MPR-mediated uptake.
30657762	10	51	theme	pathogenesis	2385:2396	arg1	types					2355:2359	two additional critical cell types	2326:2359	two additional critical cell types of Sanfilippo B disease pathogenesis	2326:2396	These results suggest that while receptor-independent mechanisms exist for lysosomal targeting of rhNAGLU in microglia, BMN 250, by its IGF2 tag moiety, confers increased CI-MPR-mediated lysosomal targeting to neurons and astrocytes, two additional critical cell types of Sanfilippo B disease pathogenesis.
30657762	10	51	theme	pathogenesis	2385:2396	arg1	neurons					2302:2308	neurons	2302:2308	neurons	2302:2308	These results suggest that while receptor-independent mechanisms exist for lysosomal targeting of rhNAGLU in microglia, BMN 250, by its IGF2 tag moiety, confers increased CI-MPR-mediated lysosomal targeting to neurons and astrocytes, two additional critical cell types of Sanfilippo B disease pathogenesis.
30657762	7	52	theme	HS	1681:1682	arg1	accumulation					1684:1695	HS accumulation	1681:1695	HS accumulation	1681:1695	Similar to neurons and astrocytes, BMN 250 uptake in Sanfilippo B patient fibroblasts is predominantly CI-MPR-mediated, resulting in augmentation of NAGLU activity with doses of enzyme that fall well below the Kuptake (5 nM), which are sufficient to prevent HS accumulation.
30657762	6	53	from	nM	1288:1289	arg1	astrocytes					1306:1315	astrocytes	1306:1315	astrocytes	1306:1315	Kuptake, defined as the concentration of enzyme at half-maximal uptake, is 5 nM and 3 nM in neurons and astrocytes, with a maximal uptake capacity (Vmax) corresponding to 764 nmol/hr/mg and 5380 nmol/hr/mg, respectively.
30657762	6	53	from	nM	1288:1289	arg1	neurons					1294:1300	neurons	1294:1300	neurons	1294:1300	Kuptake, defined as the concentration of enzyme at half-maximal uptake, is 5 nM and 3 nM in neurons and astrocytes, with a maximal uptake capacity (Vmax) corresponding to 764 nmol/hr/mg and 5380 nmol/hr/mg, respectively.
30657762	7	54	theme	B	1487:1487	arg1	fibroblasts					1497:1507	Sanfilippo B patient fibroblasts	1476:1507	Sanfilippo B patient fibroblasts	1476:1507	Similar to neurons and astrocytes, BMN 250 uptake in Sanfilippo B patient fibroblasts is predominantly CI-MPR-mediated, resulting in augmentation of NAGLU activity with doses of enzyme that fall well below the Kuptake (5 nM), which are sufficient to prevent HS accumulation.
30657762	10	55	theme	B	2375:2375	arg1	pathogenesis					2385:2396	Sanfilippo B disease pathogenesis	2364:2396	Sanfilippo B disease pathogenesis	2364:2396	These results suggest that while receptor-independent mechanisms exist for lysosomal targeting of rhNAGLU in microglia, BMN 250, by its IGF2 tag moiety, confers increased CI-MPR-mediated lysosomal targeting to neurons and astrocytes, two additional critical cell types of Sanfilippo B disease pathogenesis.
30657762	9	56	theme	BMN	2010:2012	arg1	rhNAGLU					2022:2028	rhNAGLU	2022:2028	rhNAGLU (Vmax = 12,336 nmol/hr/mg and 5469 nmol/hr/mg, respectively)	2022:2089	In microglia, receptor-independent uptake, defined as enzyme uptake resistant to competition with excess IGF2, results in appreciable lysosomal delivery of BMN 250 and rhNAGLU (Vmax = 12,336 nmol/hr/mg and 5469 nmol/hr/mg, respectively).
30657762	9	56	theme	BMN	2010:2012	arg1	delivery					1998:2005	appreciable lysosomal delivery	1976:2005	appreciable lysosomal delivery of BMN 250	1976:2016	In microglia, receptor-independent uptake, defined as enzyme uptake resistant to competition with excess IGF2, results in appreciable lysosomal delivery of BMN 250 and rhNAGLU (Vmax = 12,336 nmol/hr/mg and 5469 nmol/hr/mg, respectively).
30657762	0	57	theme	lysosomal	21:29	arg1	alpha-N-acetylglucosaminidase					31:59	lysosomal alpha-N-acetylglucosaminidase	21:59	lysosomal alpha-N-acetylglucosaminidase	21:59	BMN 250, a fusion of lysosomal alpha-N-acetylglucosaminidase with IGF2, exhibits different patterns of cellular uptake into critical cell types of Sanfilippo syndrome B disease pathogenesis.
30657762	1	58	theme	lysosomal	302:310	arg1	NAGLU					343:347	NAGLU	343:347	NAGLU	343:347	Sanfilippo syndrome type B (Sanfilippo B; Mucopolysaccharidosis type IIIB) occurs due to genetic deficiency of lysosomal alpha-N-acetylglucosaminidase (NAGLU) and subsequent lysosomal accumulation of heparan sulfate (HS), which coincides with devastating neurodegenerative disease.
30657762	1	58	theme	lysosomal	302:310	arg1	alpha-N-acetylglucosaminidase					312:340	lysosomal alpha-N-acetylglucosaminidase	302:340	lysosomal alpha-N-acetylglucosaminidase (NAGLU)	302:348	Sanfilippo syndrome type B (Sanfilippo B; Mucopolysaccharidosis type IIIB) occurs due to genetic deficiency of lysosomal alpha-N-acetylglucosaminidase (NAGLU) and subsequent lysosomal accumulation of heparan sulfate (HS), which coincides with devastating neurodegenerative disease.
30657762	9	59	theme	resistant	1922:1930	arg1	uptake					1915:1920	enzyme uptake	1908:1920	enzyme uptake resistant to competition with excess IGF2	1908:1962	In microglia, receptor-independent uptake, defined as enzyme uptake resistant to competition with excess IGF2, results in appreciable lysosomal delivery of BMN 250 and rhNAGLU (Vmax = 12,336 nmol/hr/mg and 5469 nmol/hr/mg, respectively).
30657762	6	60	with	nM	1279:1280	arg1	Vmax					1350:1353	Vmax	1350:1353	Vmax	1350:1353	Kuptake, defined as the concentration of enzyme at half-maximal uptake, is 5 nM and 3 nM in neurons and astrocytes, with a maximal uptake capacity (Vmax) corresponding to 764 nmol/hr/mg and 5380 nmol/hr/mg, respectively.
30657762	6	60	with	nM	1279:1280	arg1	capacity					1340:1347	a maximal uptake capacity	1323:1347	a maximal uptake capacity (Vmax) corresponding to 764 nmol/hr/mg and 5380 nmol/hr/mg, respectively	1323:1420	Kuptake, defined as the concentration of enzyme at half-maximal uptake, is 5 nM and 3 nM in neurons and astrocytes, with a maximal uptake capacity (Vmax) corresponding to 764 nmol/hr/mg and 5380 nmol/hr/mg, respectively.
30657762	7	61	from	uptake	1466:1471	arg1	fibroblasts					1497:1507	Sanfilippo B patient fibroblasts	1476:1507	Sanfilippo B patient fibroblasts	1476:1507	Similar to neurons and astrocytes, BMN 250 uptake in Sanfilippo B patient fibroblasts is predominantly CI-MPR-mediated, resulting in augmentation of NAGLU activity with doses of enzyme that fall well below the Kuptake (5 nM), which are sufficient to prevent HS accumulation.
30657762	5	62	theme	rodent-derived	1013:1026	arg1	neurons					1028:1034	normal rodent-derived neurons	1006:1034	normal rodent-derived neurons	1006:1034	In normal rodent-derived neurons and astrocytes, the majority of BMN250 uptake over 24 hours reaches saturation, which can be competitively inhibited with IGF2, suggestive of CI-MPR-mediated uptake.
30657762	0	63	theme	disease	169:175	arg1	pathogenesis					177:188	Sanfilippo syndrome B disease pathogenesis	147:188	Sanfilippo syndrome B disease pathogenesis	147:188	BMN 250, a fusion of lysosomal alpha-N-acetylglucosaminidase with IGF2, exhibits different patterns of cellular uptake into critical cell types of Sanfilippo syndrome B disease pathogenesis.
30657762	0	64	with	fusion	11:16	arg1	IGF2					66:69	IGF2	66:69	IGF2	66:69	BMN 250, a fusion of lysosomal alpha-N-acetylglucosaminidase with IGF2, exhibits different patterns of cellular uptake into critical cell types of Sanfilippo syndrome B disease pathogenesis.
30657762	1	65	theme	Mucopolysaccharidosis	233:253	arg1	IIIB					260:263	Mucopolysaccharidosis type IIIB	233:263	Sanfilippo B; Mucopolysaccharidosis type IIIB	219:263	Sanfilippo syndrome type B (Sanfilippo B; Mucopolysaccharidosis type IIIB) occurs due to genetic deficiency of lysosomal alpha-N-acetylglucosaminidase (NAGLU) and subsequent lysosomal accumulation of heparan sulfate (HS), which coincides with devastating neurodegenerative disease.
30657762	1	66	theme	devastating	434:444	arg1	disease					464:470	devastating neurodegenerative disease	434:470	devastating neurodegenerative disease	434:470	Sanfilippo syndrome type B (Sanfilippo B; Mucopolysaccharidosis type IIIB) occurs due to genetic deficiency of lysosomal alpha-N-acetylglucosaminidase (NAGLU) and subsequent lysosomal accumulation of heparan sulfate (HS), which coincides with devastating neurodegenerative disease.
30657762	6	67	theme	half-maximal	1253:1264	arg1	uptake					1266:1271	half-maximal uptake	1253:1271	half-maximal uptake	1253:1271	Kuptake, defined as the concentration of enzyme at half-maximal uptake, is 5 nM and 3 nM in neurons and astrocytes, with a maximal uptake capacity (Vmax) corresponding to 764 nmol/hr/mg and 5380 nmol/hr/mg, respectively.
30657762	5	68	link	rodent-derived	1013:1026	arg1	neurons					1028:1034	normal rodent-derived neurons	1006:1034	normal rodent-derived neurons	1006:1034	In normal rodent-derived neurons and astrocytes, the majority of BMN250 uptake over 24 hours reaches saturation, which can be competitively inhibited with IGF2, suggestive of CI-MPR-mediated uptake.
30657762	2	69	theme	NAGLU	620:624	arg1	molecule					626:633	an insulin-like growth factor 2 (IGF2)-tagged NAGLU molecule	574:633	an insulin-like growth factor 2 (IGF2)-tagged NAGLU molecule (BMN 250; tralesinidase alfa) that binds avidly to the IGF2 / cation-independent mannose 6-phosphate receptor (CI-MPR) for glycosylation independent lysosomal targeting	574:802	Because NAGLU expressed in Chinese hamster ovary cells is not mannose-6-phosphorylated, we developed an insulin-like growth factor 2 (IGF2)-tagged NAGLU molecule (BMN 250; tralesinidase alfa) that binds avidly to the IGF2 / cation-independent mannose 6-phosphate receptor (CI-MPR) for glycosylation independent lysosomal targeting.
30657762	2	69	theme	NAGLU	620:624	arg1	alfa					659:662	BMN 250; tralesinidase alfa	636:662	BMN 250; tralesinidase alfa	636:662	Because NAGLU expressed in Chinese hamster ovary cells is not mannose-6-phosphorylated, we developed an insulin-like growth factor 2 (IGF2)-tagged NAGLU molecule (BMN 250; tralesinidase alfa) that binds avidly to the IGF2 / cation-independent mannose 6-phosphate receptor (CI-MPR) for glycosylation independent lysosomal targeting.
30657762	1	70	theme	Sanfilippo	191:200	arg1	syndrome					202:209	Sanfilippo syndrome type B (Sanfilippo B; Mucopolysaccharidosis type IIIB)	191:264	Sanfilippo syndrome type B (Sanfilippo B; Mucopolysaccharidosis type IIIB)	191:264	Sanfilippo syndrome type B (Sanfilippo B; Mucopolysaccharidosis type IIIB) occurs due to genetic deficiency of lysosomal alpha-N-acetylglucosaminidase (NAGLU) and subsequent lysosomal accumulation of heparan sulfate (HS), which coincides with devastating neurodegenerative disease.
30657762	7	71	theme	enzyme	1601:1606	arg1	doses					1592:1596	doses	1592:1596	doses of enzyme that fall well below the Kuptake (5 nM), which are sufficient to prevent HS accumulation	1592:1695	Similar to neurons and astrocytes, BMN 250 uptake in Sanfilippo B patient fibroblasts is predominantly CI-MPR-mediated, resulting in augmentation of NAGLU activity with doses of enzyme that fall well below the Kuptake (5 nM), which are sufficient to prevent HS accumulation.
30657762	1	72	dep	B	230:230	arg1	IIIB					260:263	Mucopolysaccharidosis type IIIB	233:263	Sanfilippo B; Mucopolysaccharidosis type IIIB	219:263	Sanfilippo syndrome type B (Sanfilippo B; Mucopolysaccharidosis type IIIB) occurs due to genetic deficiency of lysosomal alpha-N-acetylglucosaminidase (NAGLU) and subsequent lysosomal accumulation of heparan sulfate (HS), which coincides with devastating neurodegenerative disease.
30657762	2	73	theme	independent	772:782	arg1	targeting					794:802	glycosylation independent lysosomal targeting	758:802	glycosylation independent lysosomal targeting	758:802	Because NAGLU expressed in Chinese hamster ovary cells is not mannose-6-phosphorylated, we developed an insulin-like growth factor 2 (IGF2)-tagged NAGLU molecule (BMN 250; tralesinidase alfa) that binds avidly to the IGF2 / cation-independent mannose 6-phosphate receptor (CI-MPR) for glycosylation independent lysosomal targeting.
30657762	0	74	theme	cell	133:136	arg1	types					138:142	critical cell types	124:142	critical cell types of Sanfilippo syndrome B disease pathogenesis	124:188	BMN 250, a fusion of lysosomal alpha-N-acetylglucosaminidase with IGF2, exhibits different patterns of cellular uptake into critical cell types of Sanfilippo syndrome B disease pathogenesis.
30657762	1	75	theme	sulfate	399:405	arg1	accumulation					375:386	subsequent lysosomal accumulation	354:386	subsequent lysosomal accumulation of heparan sulfate (HS)	354:410	Sanfilippo syndrome type B (Sanfilippo B; Mucopolysaccharidosis type IIIB) occurs due to genetic deficiency of lysosomal alpha-N-acetylglucosaminidase (NAGLU) and subsequent lysosomal accumulation of heparan sulfate (HS), which coincides with devastating neurodegenerative disease.
30657762	1	75	theme	sulfate	399:405	arg1	NAGLU					343:347	NAGLU	343:347	NAGLU	343:347	Sanfilippo syndrome type B (Sanfilippo B; Mucopolysaccharidosis type IIIB) occurs due to genetic deficiency of lysosomal alpha-N-acetylglucosaminidase (NAGLU) and subsequent lysosomal accumulation of heparan sulfate (HS), which coincides with devastating neurodegenerative disease.
30657762	1	75	theme	sulfate	399:405	arg1	alpha-N-acetylglucosaminidase					312:340	lysosomal alpha-N-acetylglucosaminidase	302:340	lysosomal alpha-N-acetylglucosaminidase (NAGLU)	302:348	Sanfilippo syndrome type B (Sanfilippo B; Mucopolysaccharidosis type IIIB) occurs due to genetic deficiency of lysosomal alpha-N-acetylglucosaminidase (NAGLU) and subsequent lysosomal accumulation of heparan sulfate (HS), which coincides with devastating neurodegenerative disease.
30657762	1	76	theme	type	211:214	arg1	B					230:230	Sanfilippo B	219:230	Sanfilippo B; Mucopolysaccharidosis type IIIB	219:263	Sanfilippo syndrome type B (Sanfilippo B; Mucopolysaccharidosis type IIIB) occurs due to genetic deficiency of lysosomal alpha-N-acetylglucosaminidase (NAGLU) and subsequent lysosomal accumulation of heparan sulfate (HS), which coincides with devastating neurodegenerative disease.
30657762	1	76	theme	type	211:214	arg1	B					216:216	type B	211:216	Sanfilippo syndrome type B (Sanfilippo B; Mucopolysaccharidosis type IIIB)	191:264	Sanfilippo syndrome type B (Sanfilippo B; Mucopolysaccharidosis type IIIB) occurs due to genetic deficiency of lysosomal alpha-N-acetylglucosaminidase (NAGLU) and subsequent lysosomal accumulation of heparan sulfate (HS), which coincides with devastating neurodegenerative disease.
30657762	4	77	theme	uptake	942:947	arg1	mechanisms					949:958	two cellular uptake mechanisms	929:958	two cellular uptake mechanisms by which BMN 250 is targeted to lysosomes	929:1000	Here we distinguish two cellular uptake mechanisms by which BMN 250 is targeted to lysosomes.
30657762	7	78	theme	activity	1578:1585	arg1	augmentation					1556:1567	augmentation	1556:1567	augmentation of NAGLU activity with doses of enzyme that fall well below the Kuptake (5 nM), which are sufficient to prevent HS accumulation	1556:1695	Similar to neurons and astrocytes, BMN 250 uptake in Sanfilippo B patient fibroblasts is predominantly CI-MPR-mediated, resulting in augmentation of NAGLU activity with doses of enzyme that fall well below the Kuptake (5 nM), which are sufficient to prevent HS accumulation.
30657762	10	79	theme	CI-MPR-mediated	2263:2277	arg1	targeting					2289:2297	increased CI-MPR-mediated lysosomal targeting	2253:2297	increased CI-MPR-mediated lysosomal targeting to neurons and astrocytes, two additional critical cell types of Sanfilippo B disease pathogenesis	2253:2396	These results suggest that while receptor-independent mechanisms exist for lysosomal targeting of rhNAGLU in microglia, BMN 250, by its IGF2 tag moiety, confers increased CI-MPR-mediated lysosomal targeting to neurons and astrocytes, two additional critical cell types of Sanfilippo B disease pathogenesis.
30657762	0	80	theme	syndrome	158:165	arg1	pathogenesis					177:188	Sanfilippo syndrome B disease pathogenesis	147:188	Sanfilippo syndrome B disease pathogenesis	147:188	BMN 250, a fusion of lysosomal alpha-N-acetylglucosaminidase with IGF2, exhibits different patterns of cellular uptake into critical cell types of Sanfilippo syndrome B disease pathogenesis.
30657762	3	81	theme	replacement	871:881	arg1	BMN					805:807	BMN 250	805:811	BMN 250	805:811	BMN 250 is currently being developed as an investigational enzyme replacement therapy for Sanfilippo B.
30657762	3	81	theme	replacement	871:881	arg1	therapy					883:889	an investigational enzyme replacement therapy	845:889	an investigational enzyme replacement therapy for Sanfilippo B	845:906	BMN 250 is currently being developed as an investigational enzyme replacement therapy for Sanfilippo B.
30657762	2	82	theme	insulin-like	577:588	arg1	factor					597:602	insulin-like growth factor 2	577:604	insulin-like growth factor 2	577:604	Because NAGLU expressed in Chinese hamster ovary cells is not mannose-6-phosphorylated, we developed an insulin-like growth factor 2 (IGF2)-tagged NAGLU molecule (BMN 250; tralesinidase alfa) that binds avidly to the IGF2 / cation-independent mannose 6-phosphate receptor (CI-MPR) for glycosylation independent lysosomal targeting.
30657762	9	83	theme	enzyme	1908:1913	arg1	uptake					1915:1920	enzyme uptake	1908:1920	enzyme uptake resistant to competition with excess IGF2	1908:1962	In microglia, receptor-independent uptake, defined as enzyme uptake resistant to competition with excess IGF2, results in appreciable lysosomal delivery of BMN 250 and rhNAGLU (Vmax = 12,336 nmol/hr/mg and 5469 nmol/hr/mg, respectively).
30657762	7	84	with	augmentation	1556:1567	arg1	doses					1592:1596	doses	1592:1596	doses of enzyme that fall well below the Kuptake (5 nM), which are sufficient to prevent HS accumulation	1592:1695	Similar to neurons and astrocytes, BMN 250 uptake in Sanfilippo B patient fibroblasts is predominantly CI-MPR-mediated, resulting in augmentation of NAGLU activity with doses of enzyme that fall well below the Kuptake (5 nM), which are sufficient to prevent HS accumulation.
30657762	3	85	theme	investigational	848:862	arg1	BMN					805:807	BMN 250	805:811	BMN 250	805:811	BMN 250 is currently being developed as an investigational enzyme replacement therapy for Sanfilippo B.
30657762	3	85	theme	investigational	848:862	arg1	therapy					883:889	an investigational enzyme replacement therapy	845:889	an investigational enzyme replacement therapy for Sanfilippo B	845:906	BMN 250 is currently being developed as an investigational enzyme replacement therapy for Sanfilippo B.
30657762	2	86	theme	hamster	508:514	arg1	cells					522:526	Chinese hamster ovary cells	500:526	Chinese hamster ovary cells	500:526	Because NAGLU expressed in Chinese hamster ovary cells is not mannose-6-phosphorylated, we developed an insulin-like growth factor 2 (IGF2)-tagged NAGLU molecule (BMN 250; tralesinidase alfa) that binds avidly to the IGF2 / cation-independent mannose 6-phosphate receptor (CI-MPR) for glycosylation independent lysosomal targeting.
30657762	2	87	theme	mannose	716:722	arg1	CI-MPR					746:751	CI-MPR	746:751	CI-MPR	746:751	Because NAGLU expressed in Chinese hamster ovary cells is not mannose-6-phosphorylated, we developed an insulin-like growth factor 2 (IGF2)-tagged NAGLU molecule (BMN 250; tralesinidase alfa) that binds avidly to the IGF2 / cation-independent mannose 6-phosphate receptor (CI-MPR) for glycosylation independent lysosomal targeting.
30657762	2	87	theme	mannose	716:722	arg1	receptor					736:743	cation-independent mannose 6-phosphate receptor	697:743	an insulin-like growth factor 2 (IGF2)-tagged NAGLU molecule (BMN 250; tralesinidase alfa) that binds avidly to the IGF2 / cation-independent mannose 6-phosphate receptor (CI-MPR) for glycosylation independent lysosomal targeting	574:802	Because NAGLU expressed in Chinese hamster ovary cells is not mannose-6-phosphorylated, we developed an insulin-like growth factor 2 (IGF2)-tagged NAGLU molecule (BMN 250; tralesinidase alfa) that binds avidly to the IGF2 / cation-independent mannose 6-phosphate receptor (CI-MPR) for glycosylation independent lysosomal targeting.
30657762	6	88	theme	uptake	1333:1338	arg1	Vmax					1350:1353	Vmax	1350:1353	Vmax	1350:1353	Kuptake, defined as the concentration of enzyme at half-maximal uptake, is 5 nM and 3 nM in neurons and astrocytes, with a maximal uptake capacity (Vmax) corresponding to 764 nmol/hr/mg and 5380 nmol/hr/mg, respectively.
30657762	6	88	theme	uptake	1333:1338	arg1	capacity					1340:1347	a maximal uptake capacity	1323:1347	a maximal uptake capacity (Vmax) corresponding to 764 nmol/hr/mg and 5380 nmol/hr/mg, respectively	1323:1420	Kuptake, defined as the concentration of enzyme at half-maximal uptake, is 5 nM and 3 nM in neurons and astrocytes, with a maximal uptake capacity (Vmax) corresponding to 764 nmol/hr/mg and 5380 nmol/hr/mg, respectively.
30657762	8	89	theme	untagged	1725:1732	arg1	enzyme					1768:1773	the untagged recombinant human NAGLU (rhNAGLU) enzyme	1721:1773	the untagged recombinant human NAGLU (rhNAGLU) enzyme	1721:1773	In contrast, uptake of the untagged recombinant human NAGLU (rhNAGLU) enzyme in neurons, astrocytes and fibroblasts is negligible at the same doses tested.
30657762	9	90	dep	rhNAGLU	2022:2028	arg1	=					2036:2036	=	2036:2036	=	2036:2036	In microglia, receptor-independent uptake, defined as enzyme uptake resistant to competition with excess IGF2, results in appreciable lysosomal delivery of BMN 250 and rhNAGLU (Vmax = 12,336 nmol/hr/mg and 5469 nmol/hr/mg, respectively).
30657762	0	91	theme	uptake	112:117	arg1	patterns					91:98	different patterns	81:98	different patterns of cellular uptake into critical cell types of Sanfilippo syndrome B disease pathogenesis	81:188	BMN 250, a fusion of lysosomal alpha-N-acetylglucosaminidase with IGF2, exhibits different patterns of cellular uptake into critical cell types of Sanfilippo syndrome B disease pathogenesis.
30657762	1	92	theme	heparan	391:397	arg1	HS					408:409	HS	408:409	HS	408:409	Sanfilippo syndrome type B (Sanfilippo B; Mucopolysaccharidosis type IIIB) occurs due to genetic deficiency of lysosomal alpha-N-acetylglucosaminidase (NAGLU) and subsequent lysosomal accumulation of heparan sulfate (HS), which coincides with devastating neurodegenerative disease.
30657762	1	92	theme	heparan	391:397	arg1	sulfate					399:405	heparan sulfate	391:405	heparan sulfate (HS)	391:410	Sanfilippo syndrome type B (Sanfilippo B; Mucopolysaccharidosis type IIIB) occurs due to genetic deficiency of lysosomal alpha-N-acetylglucosaminidase (NAGLU) and subsequent lysosomal accumulation of heparan sulfate (HS), which coincides with devastating neurodegenerative disease.
30657762	8	93	theme	human	1746:1750	arg1	enzyme					1768:1773	the untagged recombinant human NAGLU (rhNAGLU) enzyme	1721:1773	the untagged recombinant human NAGLU (rhNAGLU) enzyme	1721:1773	In contrast, uptake of the untagged recombinant human NAGLU (rhNAGLU) enzyme in neurons, astrocytes and fibroblasts is negligible at the same doses tested.
30657762	9	94	theme	excess	1952:1957	arg1	IGF2					1959:1962	excess IGF2	1952:1962	excess IGF2	1952:1962	In microglia, receptor-independent uptake, defined as enzyme uptake resistant to competition with excess IGF2, results in appreciable lysosomal delivery of BMN 250 and rhNAGLU (Vmax = 12,336 nmol/hr/mg and 5469 nmol/hr/mg, respectively).
30657762	0	95	theme	cellular	103:110	arg1	uptake					112:117	cellular uptake	103:117	cellular uptake into critical cell types of Sanfilippo syndrome B disease pathogenesis	103:188	BMN 250, a fusion of lysosomal alpha-N-acetylglucosaminidase with IGF2, exhibits different patterns of cellular uptake into critical cell types of Sanfilippo syndrome B disease pathogenesis.
30657762	1	96	theme	lysosomal	365:373	arg1	accumulation					375:386	subsequent lysosomal accumulation	354:386	subsequent lysosomal accumulation of heparan sulfate (HS)	354:410	Sanfilippo syndrome type B (Sanfilippo B; Mucopolysaccharidosis type IIIB) occurs due to genetic deficiency of lysosomal alpha-N-acetylglucosaminidase (NAGLU) and subsequent lysosomal accumulation of heparan sulfate (HS), which coincides with devastating neurodegenerative disease.
30657762	10	97	theme	rhNAGLU	2190:2196	arg1	targeting					2177:2185	lysosomal targeting	2167:2185	lysosomal targeting of rhNAGLU in microglia	2167:2209	These results suggest that while receptor-independent mechanisms exist for lysosomal targeting of rhNAGLU in microglia, BMN 250, by its IGF2 tag moiety, confers increased CI-MPR-mediated lysosomal targeting to neurons and astrocytes, two additional critical cell types of Sanfilippo B disease pathogenesis.
30657762	6	98	theme	maximal	1325:1331	arg1	Vmax					1350:1353	Vmax	1350:1353	Vmax	1350:1353	Kuptake, defined as the concentration of enzyme at half-maximal uptake, is 5 nM and 3 nM in neurons and astrocytes, with a maximal uptake capacity (Vmax) corresponding to 764 nmol/hr/mg and 5380 nmol/hr/mg, respectively.
30657762	6	98	theme	maximal	1325:1331	arg1	capacity					1340:1347	a maximal uptake capacity	1323:1347	a maximal uptake capacity (Vmax) corresponding to 764 nmol/hr/mg and 5380 nmol/hr/mg, respectively	1323:1420	Kuptake, defined as the concentration of enzyme at half-maximal uptake, is 5 nM and 3 nM in neurons and astrocytes, with a maximal uptake capacity (Vmax) corresponding to 764 nmol/hr/mg and 5380 nmol/hr/mg, respectively.
30657762	10	99	from	microglia	2201:2209	arg1	targeting					2177:2185	lysosomal targeting	2167:2185	lysosomal targeting of rhNAGLU in microglia	2167:2209	These results suggest that while receptor-independent mechanisms exist for lysosomal targeting of rhNAGLU in microglia, BMN 250, by its IGF2 tag moiety, confers increased CI-MPR-mediated lysosomal targeting to neurons and astrocytes, two additional critical cell types of Sanfilippo B disease pathogenesis.
30657762	5	100	theme	CI-MPR-mediated	1178:1192	arg1	uptake					1194:1199	CI-MPR-mediated uptake	1178:1199	CI-MPR-mediated uptake	1178:1199	In normal rodent-derived neurons and astrocytes, the majority of BMN250 uptake over 24 hours reaches saturation, which can be competitively inhibited with IGF2, suggestive of CI-MPR-mediated uptake.
30657762	10	101	theme	critical	2341:2348	arg1	types					2355:2359	two additional critical cell types	2326:2359	two additional critical cell types of Sanfilippo B disease pathogenesis	2326:2396	These results suggest that while receptor-independent mechanisms exist for lysosomal targeting of rhNAGLU in microglia, BMN 250, by its IGF2 tag moiety, confers increased CI-MPR-mediated lysosomal targeting to neurons and astrocytes, two additional critical cell types of Sanfilippo B disease pathogenesis.
30657762	10	101	theme	critical	2341:2348	arg1	neurons					2302:2308	neurons	2302:2308	neurons	2302:2308	These results suggest that while receptor-independent mechanisms exist for lysosomal targeting of rhNAGLU in microglia, BMN 250, by its IGF2 tag moiety, confers increased CI-MPR-mediated lysosomal targeting to neurons and astrocytes, two additional critical cell types of Sanfilippo B disease pathogenesis.
30657762	9	102	theme	lysosomal	1988:1996	arg1	delivery					1998:2005	appreciable lysosomal delivery	1976:2005	appreciable lysosomal delivery of BMN 250	1976:2016	In microglia, receptor-independent uptake, defined as enzyme uptake resistant to competition with excess IGF2, results in appreciable lysosomal delivery of BMN 250 and rhNAGLU (Vmax = 12,336 nmol/hr/mg and 5469 nmol/hr/mg, respectively).
30657762	9	103	theme	receptor-independent	1868:1887	arg1	uptake					1889:1894	receptor-independent uptake	1868:1894	receptor-independent uptake	1868:1894	In microglia, receptor-independent uptake, defined as enzyme uptake resistant to competition with excess IGF2, results in appreciable lysosomal delivery of BMN 250 and rhNAGLU (Vmax = 12,336 nmol/hr/mg and 5469 nmol/hr/mg, respectively).
30657762	1	104	theme	genetic	280:286	arg1	deficiency					288:297	genetic deficiency	280:297	genetic deficiency	280:297	Sanfilippo syndrome type B (Sanfilippo B; Mucopolysaccharidosis type IIIB) occurs due to genetic deficiency of lysosomal alpha-N-acetylglucosaminidase (NAGLU) and subsequent lysosomal accumulation of heparan sulfate (HS), which coincides with devastating neurodegenerative disease.
30657762	10	105	theme	disease	2377:2383	arg1	pathogenesis					2385:2396	Sanfilippo B disease pathogenesis	2364:2396	Sanfilippo B disease pathogenesis	2364:2396	These results suggest that while receptor-independent mechanisms exist for lysosomal targeting of rhNAGLU in microglia, BMN 250, by its IGF2 tag moiety, confers increased CI-MPR-mediated lysosomal targeting to neurons and astrocytes, two additional critical cell types of Sanfilippo B disease pathogenesis.
30657762	6	106	theme	764	1373:1375	arg1	nmol/hr/mg					1377:1386	764 nmol/hr/mg	1373:1386	764 nmol/hr/mg	1373:1386	Kuptake, defined as the concentration of enzyme at half-maximal uptake, is 5 nM and 3 nM in neurons and astrocytes, with a maximal uptake capacity (Vmax) corresponding to 764 nmol/hr/mg and 5380 nmol/hr/mg, respectively.
30657762	7	107	theme	patient	1489:1495	arg1	fibroblasts					1497:1507	Sanfilippo B patient fibroblasts	1476:1507	Sanfilippo B patient fibroblasts	1476:1507	Similar to neurons and astrocytes, BMN 250 uptake in Sanfilippo B patient fibroblasts is predominantly CI-MPR-mediated, resulting in augmentation of NAGLU activity with doses of enzyme that fall well below the Kuptake (5 nM), which are sufficient to prevent HS accumulation.
30657762	10	108	theme	Sanfilippo	2364:2373	arg1	B					2375:2375	Sanfilippo B	2364:2375	Sanfilippo B disease pathogenesis	2364:2396	These results suggest that while receptor-independent mechanisms exist for lysosomal targeting of rhNAGLU in microglia, BMN 250, by its IGF2 tag moiety, confers increased CI-MPR-mediated lysosomal targeting to neurons and astrocytes, two additional critical cell types of Sanfilippo B disease pathogenesis.
30657762	8	109	from	negligible	1817:1826	arg1	contrast					1701:1708	contrast	1701:1708	contrast	1701:1708	In contrast, uptake of the untagged recombinant human NAGLU (rhNAGLU) enzyme in neurons, astrocytes and fibroblasts is negligible at the same doses tested.
30657762	1	110	theme	alpha-N-acetylglucosaminidase	312:340	arg1	deficiency					288:297	genetic deficiency	280:297	genetic deficiency	280:297	Sanfilippo syndrome type B (Sanfilippo B; Mucopolysaccharidosis type IIIB) occurs due to genetic deficiency of lysosomal alpha-N-acetylglucosaminidase (NAGLU) and subsequent lysosomal accumulation of heparan sulfate (HS), which coincides with devastating neurodegenerative disease.
30657762	7	111	theme	Sanfilippo	1476:1485	arg1	fibroblasts					1497:1507	Sanfilippo B patient fibroblasts	1476:1507	Sanfilippo B patient fibroblasts	1476:1507	Similar to neurons and astrocytes, BMN 250 uptake in Sanfilippo B patient fibroblasts is predominantly CI-MPR-mediated, resulting in augmentation of NAGLU activity with doses of enzyme that fall well below the Kuptake (5 nM), which are sufficient to prevent HS accumulation.
30657762	1	112	theme	Sanfilippo	219:228	arg1	B					230:230	Sanfilippo B	219:230	Sanfilippo B; Mucopolysaccharidosis type IIIB	219:263	Sanfilippo syndrome type B (Sanfilippo B; Mucopolysaccharidosis type IIIB) occurs due to genetic deficiency of lysosomal alpha-N-acetylglucosaminidase (NAGLU) and subsequent lysosomal accumulation of heparan sulfate (HS), which coincides with devastating neurodegenerative disease.
30657762	1	112	theme	Sanfilippo	219:228	arg1	B					216:216	type B	211:216	Sanfilippo syndrome type B (Sanfilippo B; Mucopolysaccharidosis type IIIB)	191:264	Sanfilippo syndrome type B (Sanfilippo B; Mucopolysaccharidosis type IIIB) occurs due to genetic deficiency of lysosomal alpha-N-acetylglucosaminidase (NAGLU) and subsequent lysosomal accumulation of heparan sulfate (HS), which coincides with devastating neurodegenerative disease.
30657762	5	113	theme	normal	1006:1011	arg1	neurons					1028:1034	normal rodent-derived neurons	1006:1034	normal rodent-derived neurons	1006:1034	In normal rodent-derived neurons and astrocytes, the majority of BMN250 uptake over 24 hours reaches saturation, which can be competitively inhibited with IGF2, suggestive of CI-MPR-mediated uptake.
30657762	8	114	from	doses	1840:1844	arg1	negligible					1817:1826	negligible	1817:1826	negligible	1817:1826	In contrast, uptake of the untagged recombinant human NAGLU (rhNAGLU) enzyme in neurons, astrocytes and fibroblasts is negligible at the same doses tested.
30657762	1	115	dep	syndrome	202:209	arg1	B					230:230	Sanfilippo B	219:230	Sanfilippo B; Mucopolysaccharidosis type IIIB	219:263	Sanfilippo syndrome type B (Sanfilippo B; Mucopolysaccharidosis type IIIB) occurs due to genetic deficiency of lysosomal alpha-N-acetylglucosaminidase (NAGLU) and subsequent lysosomal accumulation of heparan sulfate (HS), which coincides with devastating neurodegenerative disease.
30657762	1	115	dep	syndrome	202:209	arg1	B					216:216	type B	211:216	Sanfilippo syndrome type B (Sanfilippo B; Mucopolysaccharidosis type IIIB)	191:264	Sanfilippo syndrome type B (Sanfilippo B; Mucopolysaccharidosis type IIIB) occurs due to genetic deficiency of lysosomal alpha-N-acetylglucosaminidase (NAGLU) and subsequent lysosomal accumulation of heparan sulfate (HS), which coincides with devastating neurodegenerative disease.
30657762	6	116	from	nM	1279:1280	arg1	astrocytes					1306:1315	astrocytes	1306:1315	astrocytes	1306:1315	Kuptake, defined as the concentration of enzyme at half-maximal uptake, is 5 nM and 3 nM in neurons and astrocytes, with a maximal uptake capacity (Vmax) corresponding to 764 nmol/hr/mg and 5380 nmol/hr/mg, respectively.
30657762	6	116	from	nM	1279:1280	arg1	neurons					1294:1300	neurons	1294:1300	neurons	1294:1300	Kuptake, defined as the concentration of enzyme at half-maximal uptake, is 5 nM and 3 nM in neurons and astrocytes, with a maximal uptake capacity (Vmax) corresponding to 764 nmol/hr/mg and 5380 nmol/hr/mg, respectively.
29628257	0	0	theme	glycocalyx-mimetic	83:100	arg1	surfaces					108:115	new glycocalyx-mimetic model surfaces	79:115	new glycocalyx-mimetic model surfaces	79:115	Atomic force microscopy of adsorbed proteoglycan mimetic nanoparticles: Toward new glycocalyx-mimetic model surfaces.
29628257	6	1	theme	tapping	765:771	arg1	microscopy					786:795	PeakForce tapping atomic force microscopy	755:795	PeakForce tapping atomic force microscopy	755:795	The surfaces are characterized by PeakForce tapping atomic force microscopy, both in air and in aqueous pH 7.4 buffer, and by PeakForce quantitative nanomechanics (PF-QNM) mode with high spatial resolution.
29628257	6	2	theme	pH	825:826	arg1	buffer					832:837	aqueous pH 7.4 buffer	817:837	aqueous pH 7.4 buffer	817:837	The surfaces are characterized by PeakForce tapping atomic force microscopy, both in air and in aqueous pH 7.4 buffer, and by PeakForce quantitative nanomechanics (PF-QNM) mode with high spatial resolution.
29628257	0	3	theme	new	79:81	arg1	surfaces					108:115	new glycocalyx-mimetic model surfaces	79:115	new glycocalyx-mimetic model surfaces	79:115	Atomic force microscopy of adsorbed proteoglycan mimetic nanoparticles: Toward new glycocalyx-mimetic model surfaces.
29628257	6	4	with	mode	893:896	arg1	resolution					916:925	high spatial resolution	903:925	high spatial resolution	903:925	The surfaces are characterized by PeakForce tapping atomic force microscopy, both in air and in aqueous pH 7.4 buffer, and by PeakForce quantitative nanomechanics (PF-QNM) mode with high spatial resolution.
29628257	4	5	theme	dense	443:447	arg1	models					480:485	models	480:485	models of the vascular endothelial glycocalyx	480:524	We report new methods for preparing and characterizing dense GAG surfaces that can serve as models of the vascular endothelial glycocalyx.
29628257	4	5	theme	dense	443:447	arg1	surfaces					453:460	dense GAG surfaces	443:460	dense GAG surfaces that can serve as models of the vascular endothelial glycocalyx	443:524	We report new methods for preparing and characterizing dense GAG surfaces that can serve as models of the vascular endothelial glycocalyx.
29628257	7	6	theme	new	934:936	arg1	surfaces					938:945	These new surfaces	928:945	These new surfaces	928:945	These new surfaces provide access to heparin-rich or chondroitin sulfate-rich coatings that mimic both composition and nanoscale structural features of the vascular endothelial glycocalyx.
29628257	7	7	theme	nanoscale	1047:1055	arg1	features					1068:1075	nanoscale structural features	1047:1075	nanoscale structural features of the vascular endothelial glycocalyx	1047:1114	These new surfaces provide access to heparin-rich or chondroitin sulfate-rich coatings that mimic both composition and nanoscale structural features of the vascular endothelial glycocalyx.
29628257	5	8	theme	chitosan-hyaluronan	665:683	arg1	PEMs					714:717	PEMs	714:717	PEMs	714:717	The GAG-rich surfaces are prepared by adsorbing heparin or chondroitin sulfate-containing polyelectrolyte complex nanoparticles (PCNs) to chitosan-hyaluronan polyelectrolyte multilayers (PEMs).
29628257	5	8	theme	chitosan-hyaluronan	665:683	arg1	multilayers					701:711	chitosan-hyaluronan polyelectrolyte multilayers	665:711	chitosan-hyaluronan polyelectrolyte multilayers (PEMs)	665:718	The GAG-rich surfaces are prepared by adsorbing heparin or chondroitin sulfate-containing polyelectrolyte complex nanoparticles (PCNs) to chitosan-hyaluronan polyelectrolyte multilayers (PEMs).
29628257	0	9	theme	model	102:106	arg1	surfaces					108:115	new glycocalyx-mimetic model surfaces	79:115	new glycocalyx-mimetic model surfaces	79:115	Atomic force microscopy of adsorbed proteoglycan mimetic nanoparticles: Toward new glycocalyx-mimetic model surfaces.
29628257	4	10	theme	glycocalyx	515:524	arg1	models					480:485	models	480:485	models of the vascular endothelial glycocalyx	480:524	We report new methods for preparing and characterizing dense GAG surfaces that can serve as models of the vascular endothelial glycocalyx.
29628257	4	10	theme	glycocalyx	515:524	arg1	surfaces					453:460	dense GAG surfaces	443:460	dense GAG surfaces that can serve as models of the vascular endothelial glycocalyx	443:524	We report new methods for preparing and characterizing dense GAG surfaces that can serve as models of the vascular endothelial glycocalyx.
29628257	5	11	theme	polyelectrolyte	685:699	arg1	PEMs					714:717	PEMs	714:717	PEMs	714:717	The GAG-rich surfaces are prepared by adsorbing heparin or chondroitin sulfate-containing polyelectrolyte complex nanoparticles (PCNs) to chitosan-hyaluronan polyelectrolyte multilayers (PEMs).
29628257	5	11	theme	polyelectrolyte	685:699	arg1	multilayers					701:711	chitosan-hyaluronan polyelectrolyte multilayers	665:711	chitosan-hyaluronan polyelectrolyte multilayers (PEMs)	665:718	The GAG-rich surfaces are prepared by adsorbing heparin or chondroitin sulfate-containing polyelectrolyte complex nanoparticles (PCNs) to chitosan-hyaluronan polyelectrolyte multilayers (PEMs).
29628257	1	12	dep	dense	142:146	arg1	non-uniform					149:159	non-uniform	149:159	non-uniform	149:159	Blood vessels present a dense, non-uniform, polysaccharide-rich layer, called the endothelial glycocalyx.
29628257	1	12	dep	dense	142:146	arg1	polysaccharide-rich					162:180	polysaccharide-rich	162:180	polysaccharide-rich	162:180	Blood vessels present a dense, non-uniform, polysaccharide-rich layer, called the endothelial glycocalyx.
29628257	5	13	theme	GAG-rich	531:538	arg1	surfaces					540:547	The GAG-rich surfaces	527:547	The GAG-rich surfaces	527:547	The GAG-rich surfaces are prepared by adsorbing heparin or chondroitin sulfate-containing polyelectrolyte complex nanoparticles (PCNs) to chitosan-hyaluronan polyelectrolyte multilayers (PEMs).
29628257	6	14	theme	force	780:784	arg1	microscopy					786:795	PeakForce tapping atomic force microscopy	755:795	PeakForce tapping atomic force microscopy	755:795	The surfaces are characterized by PeakForce tapping atomic force microscopy, both in air and in aqueous pH 7.4 buffer, and by PeakForce quantitative nanomechanics (PF-QNM) mode with high spatial resolution.
29628257	4	15	theme	endothelial	503:513	arg1	glycocalyx					515:524	the vascular endothelial glycocalyx	490:524	the vascular endothelial glycocalyx	490:524	We report new methods for preparing and characterizing dense GAG surfaces that can serve as models of the vascular endothelial glycocalyx.
29628257	4	16	theme	GAG	449:451	arg1	models					480:485	models	480:485	models of the vascular endothelial glycocalyx	480:524	We report new methods for preparing and characterizing dense GAG surfaces that can serve as models of the vascular endothelial glycocalyx.
29628257	4	16	theme	GAG	449:451	arg1	surfaces					453:460	dense GAG surfaces	443:460	dense GAG surfaces that can serve as models of the vascular endothelial glycocalyx	443:524	We report new methods for preparing and characterizing dense GAG surfaces that can serve as models of the vascular endothelial glycocalyx.
29628257	2	17	from	polysaccharides	228:242	arg1	glycocalyx					251:260	the glycocalyx	247:260	the glycocalyx	247:260	The polysaccharides in the glycocalyx include polyanionic glycosaminoglycans (GAGs).
29628257	6	18	theme	PeakForce	847:855	arg1	mode					893:896	PeakForce quantitative nanomechanics (PF-QNM) mode	847:896	PeakForce quantitative nanomechanics (PF-QNM) mode with high spatial resolution	847:925	The surfaces are characterized by PeakForce tapping atomic force microscopy, both in air and in aqueous pH 7.4 buffer, and by PeakForce quantitative nanomechanics (PF-QNM) mode with high spatial resolution.
29628257	0	19	theme	force	7:11	arg1	microscopy					13:22	Atomic force microscopy	0:22	Atomic force microscopy of adsorbed proteoglycan mimetic nanoparticles: Toward new glycocalyx-mimetic model surfaces.	0:116	Atomic force microscopy of adsorbed proteoglycan mimetic nanoparticles: Toward new glycocalyx-mimetic model surfaces.
29628257	3	20	theme	unique	360:365	arg1	compatibility					373:385	excellent and unique blood compatibility	346:385	excellent and unique blood compatibility	346:385	This polysaccharide-rich surface has excellent and unique blood compatibility.
29628257	3	21	contain	has	342:344	arg1	surface					334:340	This polysaccharide-rich surface	309:340	This polysaccharide-rich surface	309:340	This polysaccharide-rich surface has excellent and unique blood compatibility.
29628257	3	21	contain	has	342:344	arg2	compatibility					373:385	excellent and unique blood compatibility	346:385	excellent and unique blood compatibility	346:385	This polysaccharide-rich surface has excellent and unique blood compatibility.
29628257	0	22	theme	Atomic	0:5	arg1	microscopy					13:22	Atomic force microscopy	0:22	Atomic force microscopy of adsorbed proteoglycan mimetic nanoparticles: Toward new glycocalyx-mimetic model surfaces.	0:116	Atomic force microscopy of adsorbed proteoglycan mimetic nanoparticles: Toward new glycocalyx-mimetic model surfaces.
29628257	7	23	theme	vascular	1084:1091	arg1	glycocalyx					1105:1114	the vascular endothelial glycocalyx	1080:1114	the vascular endothelial glycocalyx	1080:1114	These new surfaces provide access to heparin-rich or chondroitin sulfate-rich coatings that mimic both composition and nanoscale structural features of the vascular endothelial glycocalyx.
29628257	7	24	theme	sulfate-rich	993:1004	arg1	coatings					1006:1013	heparin-rich or chondroitin sulfate-rich coatings	965:1013	heparin-rich or chondroitin sulfate-rich coatings that mimic both composition and nanoscale structural features of the vascular endothelial glycocalyx	965:1114	These new surfaces provide access to heparin-rich or chondroitin sulfate-rich coatings that mimic both composition and nanoscale structural features of the vascular endothelial glycocalyx.
29628257	5	25	dep	heparin	575:581	arg1	PCNs					656:659	PCNs	656:659	PCNs	656:659	The GAG-rich surfaces are prepared by adsorbing heparin or chondroitin sulfate-containing polyelectrolyte complex nanoparticles (PCNs) to chitosan-hyaluronan polyelectrolyte multilayers (PEMs).
29628257	5	25	dep	heparin	575:581	arg1	nanoparticles					641:653	sulfate-containing polyelectrolyte complex nanoparticles	598:653	sulfate-containing polyelectrolyte complex nanoparticles (PCNs) to chitosan-hyaluronan polyelectrolyte multilayers (PEMs)	598:718	The GAG-rich surfaces are prepared by adsorbing heparin or chondroitin sulfate-containing polyelectrolyte complex nanoparticles (PCNs) to chitosan-hyaluronan polyelectrolyte multilayers (PEMs).
29628257	6	26	theme	quantitative	857:868	arg1	mode					893:896	PeakForce quantitative nanomechanics (PF-QNM) mode	847:896	PeakForce quantitative nanomechanics (PF-QNM) mode with high spatial resolution	847:925	The surfaces are characterized by PeakForce tapping atomic force microscopy, both in air and in aqueous pH 7.4 buffer, and by PeakForce quantitative nanomechanics (PF-QNM) mode with high spatial resolution.
29628257	6	27	theme	PF-QNM	885:890	arg1	mode					893:896	PeakForce quantitative nanomechanics (PF-QNM) mode	847:896	PeakForce quantitative nanomechanics (PF-QNM) mode with high spatial resolution	847:925	The surfaces are characterized by PeakForce tapping atomic force microscopy, both in air and in aqueous pH 7.4 buffer, and by PeakForce quantitative nanomechanics (PF-QNM) mode with high spatial resolution.
29628257	1	28	theme	endothelial	200:210	arg1	glycocalyx					212:221	the endothelial glycocalyx	196:221	the endothelial glycocalyx	196:221	Blood vessels present a dense, non-uniform, polysaccharide-rich layer, called the endothelial glycocalyx.
29628257	5	29	theme	polyelectrolyte	617:631	arg1	PCNs					656:659	PCNs	656:659	PCNs	656:659	The GAG-rich surfaces are prepared by adsorbing heparin or chondroitin sulfate-containing polyelectrolyte complex nanoparticles (PCNs) to chitosan-hyaluronan polyelectrolyte multilayers (PEMs).
29628257	5	29	theme	polyelectrolyte	617:631	arg1	nanoparticles					641:653	sulfate-containing polyelectrolyte complex nanoparticles	598:653	sulfate-containing polyelectrolyte complex nanoparticles (PCNs) to chitosan-hyaluronan polyelectrolyte multilayers (PEMs)	598:718	The GAG-rich surfaces are prepared by adsorbing heparin or chondroitin sulfate-containing polyelectrolyte complex nanoparticles (PCNs) to chitosan-hyaluronan polyelectrolyte multilayers (PEMs).
29628257	1	30	theme	Blood	118:122	arg1	vessels					124:130	Blood vessels	118:130	Blood vessels	118:130	Blood vessels present a dense, non-uniform, polysaccharide-rich layer, called the endothelial glycocalyx.
29628257	6	31	theme	PeakForce	755:763	arg1	microscopy					786:795	PeakForce tapping atomic force microscopy	755:795	PeakForce tapping atomic force microscopy	755:795	The surfaces are characterized by PeakForce tapping atomic force microscopy, both in air and in aqueous pH 7.4 buffer, and by PeakForce quantitative nanomechanics (PF-QNM) mode with high spatial resolution.
29628257	6	32	theme	atomic	773:778	arg1	microscopy					786:795	PeakForce tapping atomic force microscopy	755:795	PeakForce tapping atomic force microscopy	755:795	The surfaces are characterized by PeakForce tapping atomic force microscopy, both in air and in aqueous pH 7.4 buffer, and by PeakForce quantitative nanomechanics (PF-QNM) mode with high spatial resolution.
29628257	0	33	theme	proteoglycan	36:47	arg1	nanoparticles					57:69	adsorbed proteoglycan mimetic nanoparticles	27:69	adsorbed proteoglycan mimetic nanoparticles	27:69	Atomic force microscopy of adsorbed proteoglycan mimetic nanoparticles: Toward new glycocalyx-mimetic model surfaces.
29628257	0	34	theme	adsorbed	27:34	arg1	nanoparticles					57:69	adsorbed proteoglycan mimetic nanoparticles	27:69	adsorbed proteoglycan mimetic nanoparticles	27:69	Atomic force microscopy of adsorbed proteoglycan mimetic nanoparticles: Toward new glycocalyx-mimetic model surfaces.
29628257	4	35	theme	vascular	494:501	arg1	glycocalyx					515:524	the vascular endothelial glycocalyx	490:524	the vascular endothelial glycocalyx	490:524	We report new methods for preparing and characterizing dense GAG surfaces that can serve as models of the vascular endothelial glycocalyx.
29628257	7	36	theme	glycocalyx	1105:1114	arg1	composition					1031:1041	composition	1031:1041	composition	1031:1041	These new surfaces provide access to heparin-rich or chondroitin sulfate-rich coatings that mimic both composition and nanoscale structural features of the vascular endothelial glycocalyx.
29628257	7	36	theme	glycocalyx	1105:1114	arg1	features					1068:1075	nanoscale structural features	1047:1075	nanoscale structural features of the vascular endothelial glycocalyx	1047:1114	These new surfaces provide access to heparin-rich or chondroitin sulfate-rich coatings that mimic both composition and nanoscale structural features of the vascular endothelial glycocalyx.
29628257	0	37	theme	nanoparticles	57:69	arg1	microscopy					13:22	Atomic force microscopy	0:22	Atomic force microscopy of adsorbed proteoglycan mimetic nanoparticles: Toward new glycocalyx-mimetic model surfaces.	0:116	Atomic force microscopy of adsorbed proteoglycan mimetic nanoparticles: Toward new glycocalyx-mimetic model surfaces.
29628257	6	38	theme	nanomechanics	870:882	arg1	mode					893:896	PeakForce quantitative nanomechanics (PF-QNM) mode	847:896	PeakForce quantitative nanomechanics (PF-QNM) mode with high spatial resolution	847:925	The surfaces are characterized by PeakForce tapping atomic force microscopy, both in air and in aqueous pH 7.4 buffer, and by PeakForce quantitative nanomechanics (PF-QNM) mode with high spatial resolution.
29628257	6	39	theme	spatial	908:914	arg1	resolution					916:925	high spatial resolution	903:925	high spatial resolution	903:925	The surfaces are characterized by PeakForce tapping atomic force microscopy, both in air and in aqueous pH 7.4 buffer, and by PeakForce quantitative nanomechanics (PF-QNM) mode with high spatial resolution.
29628257	5	40	theme	sulfate-containing	598:615	arg1	PCNs					656:659	PCNs	656:659	PCNs	656:659	The GAG-rich surfaces are prepared by adsorbing heparin or chondroitin sulfate-containing polyelectrolyte complex nanoparticles (PCNs) to chitosan-hyaluronan polyelectrolyte multilayers (PEMs).
29628257	5	40	theme	sulfate-containing	598:615	arg1	nanoparticles					641:653	sulfate-containing polyelectrolyte complex nanoparticles	598:653	sulfate-containing polyelectrolyte complex nanoparticles (PCNs) to chitosan-hyaluronan polyelectrolyte multilayers (PEMs)	598:718	The GAG-rich surfaces are prepared by adsorbing heparin or chondroitin sulfate-containing polyelectrolyte complex nanoparticles (PCNs) to chitosan-hyaluronan polyelectrolyte multilayers (PEMs).
29628257	0	41	theme	mimetic	49:55	arg1	nanoparticles					57:69	adsorbed proteoglycan mimetic nanoparticles	27:69	adsorbed proteoglycan mimetic nanoparticles	27:69	Atomic force microscopy of adsorbed proteoglycan mimetic nanoparticles: Toward new glycocalyx-mimetic model surfaces.
29628257	3	42	theme	excellent	346:354	arg1	compatibility					373:385	excellent and unique blood compatibility	346:385	excellent and unique blood compatibility	346:385	This polysaccharide-rich surface has excellent and unique blood compatibility.
29628257	6	43	theme	high	903:906	arg1	resolution					916:925	high spatial resolution	903:925	high spatial resolution	903:925	The surfaces are characterized by PeakForce tapping atomic force microscopy, both in air and in aqueous pH 7.4 buffer, and by PeakForce quantitative nanomechanics (PF-QNM) mode with high spatial resolution.
29628257	4	44	theme	new	398:400	arg1	methods					402:408	new methods	398:408	new methods for preparing and characterizing dense GAG surfaces that can serve as models of the vascular endothelial glycocalyx	398:524	We report new methods for preparing and characterizing dense GAG surfaces that can serve as models of the vascular endothelial glycocalyx.
29628257	3	45	theme	polysaccharide-rich	314:332	arg1	surface					334:340	This polysaccharide-rich surface	309:340	This polysaccharide-rich surface	309:340	This polysaccharide-rich surface has excellent and unique blood compatibility.
29628257	5	46	theme	complex	633:639	arg1	PCNs					656:659	PCNs	656:659	PCNs	656:659	The GAG-rich surfaces are prepared by adsorbing heparin or chondroitin sulfate-containing polyelectrolyte complex nanoparticles (PCNs) to chitosan-hyaluronan polyelectrolyte multilayers (PEMs).
29628257	5	46	theme	complex	633:639	arg1	nanoparticles					641:653	sulfate-containing polyelectrolyte complex nanoparticles	598:653	sulfate-containing polyelectrolyte complex nanoparticles (PCNs) to chitosan-hyaluronan polyelectrolyte multilayers (PEMs)	598:718	The GAG-rich surfaces are prepared by adsorbing heparin or chondroitin sulfate-containing polyelectrolyte complex nanoparticles (PCNs) to chitosan-hyaluronan polyelectrolyte multilayers (PEMs).
29628257	7	47	theme	chondroitin	981:991	arg1	coatings					1006:1013	heparin-rich or chondroitin sulfate-rich coatings	965:1013	heparin-rich or chondroitin sulfate-rich coatings that mimic both composition and nanoscale structural features of the vascular endothelial glycocalyx	965:1114	These new surfaces provide access to heparin-rich or chondroitin sulfate-rich coatings that mimic both composition and nanoscale structural features of the vascular endothelial glycocalyx.
29628257	6	48	theme	aqueous	817:823	arg1	buffer					832:837	aqueous pH 7.4 buffer	817:837	aqueous pH 7.4 buffer	817:837	The surfaces are characterized by PeakForce tapping atomic force microscopy, both in air and in aqueous pH 7.4 buffer, and by PeakForce quantitative nanomechanics (PF-QNM) mode with high spatial resolution.
29628257	1	49	theme	dense	142:146	arg1	layer					182:186	a dense, non-uniform, polysaccharide-rich layer	140:186	a dense, non-uniform, polysaccharide-rich layer	140:186	Blood vessels present a dense, non-uniform, polysaccharide-rich layer, called the endothelial glycocalyx.
29628257	3	50	theme	blood	367:371	arg1	compatibility					373:385	excellent and unique blood compatibility	346:385	excellent and unique blood compatibility	346:385	This polysaccharide-rich surface has excellent and unique blood compatibility.
29628257	7	51	theme	endothelial	1093:1103	arg1	glycocalyx					1105:1114	the vascular endothelial glycocalyx	1080:1114	the vascular endothelial glycocalyx	1080:1114	These new surfaces provide access to heparin-rich or chondroitin sulfate-rich coatings that mimic both composition and nanoscale structural features of the vascular endothelial glycocalyx.
29628257	2	52	theme	polyanionic	270:280	arg1	GAGs					302:305	GAGs	302:305	GAGs	302:305	The polysaccharides in the glycocalyx include polyanionic glycosaminoglycans (GAGs).
29628257	2	52	theme	polyanionic	270:280	arg1	glycosaminoglycans					282:299	polyanionic glycosaminoglycans	270:299	polyanionic glycosaminoglycans (GAGs)	270:306	The polysaccharides in the glycocalyx include polyanionic glycosaminoglycans (GAGs).
29628257	5	53	theme	adsorbing	565:573	arg1	heparin					575:581	adsorbing heparin	565:581	adsorbing heparin	565:581	The GAG-rich surfaces are prepared by adsorbing heparin or chondroitin sulfate-containing polyelectrolyte complex nanoparticles (PCNs) to chitosan-hyaluronan polyelectrolyte multilayers (PEMs).
29628257	7	54	theme	heparin-rich	965:976	arg1	coatings					1006:1013	heparin-rich or chondroitin sulfate-rich coatings	965:1013	heparin-rich or chondroitin sulfate-rich coatings that mimic both composition and nanoscale structural features of the vascular endothelial glycocalyx	965:1114	These new surfaces provide access to heparin-rich or chondroitin sulfate-rich coatings that mimic both composition and nanoscale structural features of the vascular endothelial glycocalyx.
29628257	7	55	theme	structural	1057:1066	arg1	features					1068:1075	nanoscale structural features	1047:1075	nanoscale structural features of the vascular endothelial glycocalyx	1047:1114	These new surfaces provide access to heparin-rich or chondroitin sulfate-rich coatings that mimic both composition and nanoscale structural features of the vascular endothelial glycocalyx.
30925988	0	0	theme	tunable	92:98	arg1	properties					111:120	tunable mechanical properties	92:120	tunable mechanical properties	92:120	The ternary system amylose-amylopectin-formic acid as precursor for electrospun fibers with tunable mechanical properties.
30925988	4	1	from	effect	511:516	arg1	spinnability					593:604	spinnability	593:604	spinnability of solutions	593:617	This approach allowed us to focus on the effect of the polysaccharides ratio on phase behavior, rheological properties and spinnability of solutions and eliminate the effect of molecular characteristics of the polymers.
30925988	4	1	from	effect	511:516	arg1	behavior					556:563	phase behavior	550:563	phase behavior	550:563	This approach allowed us to focus on the effect of the polysaccharides ratio on phase behavior, rheological properties and spinnability of solutions and eliminate the effect of molecular characteristics of the polymers.
30925988	4	1	from	effect	511:516	arg1	properties					578:587	rheological properties	566:587	rheological properties	566:587	This approach allowed us to focus on the effect of the polysaccharides ratio on phase behavior, rheological properties and spinnability of solutions and eliminate the effect of molecular characteristics of the polymers.
30925988	7	2	theme	Amylose-rich	913:924	arg1	fibers					926:931	Amylose-rich fibers	913:931	Amylose-rich fibers	913:931	Amylose-rich fibers demonstrated higher strength, stiffness and ductility, while amylopectin-rich fibers were weak and brittle.
30925988	7	3	dep	higher	946:951	arg1	ductility					977:985	ductility	977:985	ductility	977:985	Amylose-rich fibers demonstrated higher strength, stiffness and ductility, while amylopectin-rich fibers were weak and brittle.
30925988	7	3	dep	higher	946:951	arg1	stiffness					963:971	stiffness	963:971	stiffness	963:971	Amylose-rich fibers demonstrated higher strength, stiffness and ductility, while amylopectin-rich fibers were weak and brittle.
30925988	7	3	dep	higher	946:951	arg1	strength					953:960	strength	953:960	strength	953:960	Amylose-rich fibers demonstrated higher strength, stiffness and ductility, while amylopectin-rich fibers were weak and brittle.
30925988	8	4	theme	mechanical	1060:1069	arg1	properties					1071:1080	mechanical properties	1060:1080	mechanical properties	1060:1080	This difference in mechanical properties marks possible fields of application of these two types of materials.
30925988	7	5	theme	amylopectin-rich	994:1009	arg1	fibers					1011:1016	amylopectin-rich fibers	994:1016	amylopectin-rich fibers	994:1016	Amylose-rich fibers demonstrated higher strength, stiffness and ductility, while amylopectin-rich fibers were weak and brittle.
30925988	1	6	theme	potential	179:187	arg1	utility					189:195	potential utility	179:195	potential utility in many fields	179:210	Electrospun starch fibers are promising materials, with potential utility in many fields, including food packaging and tissue engineering.
30925988	2	7	used	used	286:289	arg2	we					283:284	we	283:284	we	283:284	In the present work, we used formylated derivatives of starch constituents, amylose and amylopectin.
30925988	4	8	theme	rheological	566:576	arg1	properties					578:587	rheological properties	566:587	rheological properties	566:587	This approach allowed us to focus on the effect of the polysaccharides ratio on phase behavior, rheological properties and spinnability of solutions and eliminate the effect of molecular characteristics of the polymers.
30925988	1	9	with	materials	163:171	arg1	utility					189:195	potential utility	179:195	potential utility in many fields	179:210	Electrospun starch fibers are promising materials, with potential utility in many fields, including food packaging and tissue engineering.
30925988	6	10	theme	higher	833:838	arg1	elongation					840:849	much higher elongation	828:849	much higher elongation at break	828:858	All the fibers were largely amorphous and exhibited much higher elongation at break when compared to cast films of the same composition.
30925988	2	11	theme	formylated	291:300	arg1	amylopectin					350:360	amylopectin	350:360	amylopectin	350:360	In the present work, we used formylated derivatives of starch constituents, amylose and amylopectin.
30925988	2	11	theme	formylated	291:300	arg1	amylose					338:344	amylose	338:344	amylose	338:344	In the present work, we used formylated derivatives of starch constituents, amylose and amylopectin.
30925988	2	11	theme	formylated	291:300	arg1	derivatives					302:312	formylated derivatives	291:312	formylated derivatives of starch constituents	291:335	In the present work, we used formylated derivatives of starch constituents, amylose and amylopectin.
30925988	0	12	theme	mechanical	100:109	arg1	properties					111:120	tunable mechanical properties	92:120	tunable mechanical properties	92:120	The ternary system amylose-amylopectin-formic acid as precursor for electrospun fibers with tunable mechanical properties.
30925988	0	13	with	fibers	80:85	arg1	properties					111:120	tunable mechanical properties	92:120	tunable mechanical properties	92:120	The ternary system amylose-amylopectin-formic acid as precursor for electrospun fibers with tunable mechanical properties.
30925988	4	14	theme	molecular	647:655	arg1	characteristics					657:671	molecular characteristics	647:671	molecular characteristics of the polymers	647:687	This approach allowed us to focus on the effect of the polysaccharides ratio on phase behavior, rheological properties and spinnability of solutions and eliminate the effect of molecular characteristics of the polymers.
30925988	9	15	theme	tissue	1237:1242	arg1	engineering					1244:1254	tissue engineering	1237:1254	tissue engineering	1237:1254	Fibers with high amylose content may have potential application in food packaging or tissue engineering while fibers with high amylopectin concentration are in favor for control drug release.
30925988	4	16	theme	polymers	680:687	arg1	characteristics					657:671	molecular characteristics	647:671	molecular characteristics of the polymers	647:687	This approach allowed us to focus on the effect of the polysaccharides ratio on phase behavior, rheological properties and spinnability of solutions and eliminate the effect of molecular characteristics of the polymers.
30925988	3	17	theme	starch	447:452	arg1	behavior					435:442	the behavior	431:442	the behavior of starch in formic acid	431:467	Various blends of amylose and amylopectin were used to characterize the behavior of starch in formic acid.
30925988	3	18	theme	Various	363:369	arg1	blends					371:376	Various blends	363:376	Various blends of amylose and amylopectin	363:403	Various blends of amylose and amylopectin were used to characterize the behavior of starch in formic acid.
30925988	6	19	theme	composition	900:910	arg1	films					882:886	cast films	877:886	cast films of the same composition	877:910	All the fibers were largely amorphous and exhibited much higher elongation at break when compared to cast films of the same composition.
30925988	9	20	theme	control	1322:1328	arg1	release					1335:1341	control drug release	1322:1341	control drug release	1322:1341	Fibers with high amylose content may have potential application in food packaging or tissue engineering while fibers with high amylopectin concentration are in favor for control drug release.
30925988	4	21	theme	characteristics	657:671	arg1	effect					637:642	the effect	633:642	the effect of molecular characteristics of the polymers	633:687	This approach allowed us to focus on the effect of the polysaccharides ratio on phase behavior, rheological properties and spinnability of solutions and eliminate the effect of molecular characteristics of the polymers.
30925988	0	22	theme	ternary	4:10	arg1	acid					46:49	The ternary system amylose-amylopectin-formic acid	0:49	The ternary system amylose-amylopectin-formic acid	0:49	The ternary system amylose-amylopectin-formic acid as precursor for electrospun fibers with tunable mechanical properties.
30925988	6	23	theme	same	895:898	arg1	composition					900:910	the same composition	891:910	the same composition	891:910	All the fibers were largely amorphous and exhibited much higher elongation at break when compared to cast films of the same composition.
30925988	9	24	with	fibers	1262:1267	arg1	concentration					1291:1303	high amylopectin concentration	1274:1303	high amylopectin concentration	1274:1303	Fibers with high amylose content may have potential application in food packaging or tissue engineering while fibers with high amylopectin concentration are in favor for control drug release.
30925988	2	25	theme	constituents	324:335	arg1	amylopectin					350:360	amylopectin	350:360	amylopectin	350:360	In the present work, we used formylated derivatives of starch constituents, amylose and amylopectin.
30925988	2	25	theme	constituents	324:335	arg1	amylose					338:344	amylose	338:344	amylose	338:344	In the present work, we used formylated derivatives of starch constituents, amylose and amylopectin.
30925988	2	25	theme	constituents	324:335	arg1	derivatives					302:312	formylated derivatives	291:312	formylated derivatives of starch constituents	291:335	In the present work, we used formylated derivatives of starch constituents, amylose and amylopectin.
30925988	8	26	theme	materials	1141:1149	arg1	types					1132:1136	these two types	1122:1136	these two types of materials	1122:1149	This difference in mechanical properties marks possible fields of application of these two types of materials.
30925988	1	27	theme	many	200:203	arg1	fields					205:210	many fields	200:210	many fields	200:210	Electrospun starch fibers are promising materials, with potential utility in many fields, including food packaging and tissue engineering.
30925988	4	28	theme	phase	550:554	arg1	behavior					556:563	phase behavior	550:563	phase behavior	550:563	This approach allowed us to focus on the effect of the polysaccharides ratio on phase behavior, rheological properties and spinnability of solutions and eliminate the effect of molecular characteristics of the polymers.
30925988	8	29	theme	application	1107:1117	arg1	fields					1097:1102	possible fields	1088:1102	possible fields of application of these two types of materials	1088:1149	This difference in mechanical properties marks possible fields of application of these two types of materials.
30925988	2	30	theme	starch	317:322	arg1	constituents					324:335	starch constituents	317:335	starch constituents	317:335	In the present work, we used formylated derivatives of starch constituents, amylose and amylopectin.
30925988	9	31	theme	amylopectin	1279:1289	arg1	concentration					1291:1303	high amylopectin concentration	1274:1303	high amylopectin concentration	1274:1303	Fibers with high amylose content may have potential application in food packaging or tissue engineering while fibers with high amylopectin concentration are in favor for control drug release.
30925988	0	32	theme	amylose-amylopectin-formic	19:44	arg1	acid					46:49	The ternary system amylose-amylopectin-formic acid	0:49	The ternary system amylose-amylopectin-formic acid	0:49	The ternary system amylose-amylopectin-formic acid as precursor for electrospun fibers with tunable mechanical properties.
30925988	9	33	theme	food	1219:1222	arg1	packaging					1224:1232	food packaging	1219:1232	food packaging	1219:1232	Fibers with high amylose content may have potential application in food packaging or tissue engineering while fibers with high amylopectin concentration are in favor for control drug release.
30925988	0	34	theme	system	12:17	arg1	acid					46:49	The ternary system amylose-amylopectin-formic acid	0:49	The ternary system amylose-amylopectin-formic acid	0:49	The ternary system amylose-amylopectin-formic acid as precursor for electrospun fibers with tunable mechanical properties.
30925988	9	35	theme	high	1274:1277	arg1	concentration					1291:1303	high amylopectin concentration	1274:1303	high amylopectin concentration	1274:1303	Fibers with high amylose content may have potential application in food packaging or tissue engineering while fibers with high amylopectin concentration are in favor for control drug release.
30925988	1	36	theme	Electrospun	123:133	arg1	engineering					249:259	tissue engineering	242:259	tissue engineering	242:259	Electrospun starch fibers are promising materials, with potential utility in many fields, including food packaging and tissue engineering.
30925988	1	36	theme	Electrospun	123:133	arg1	fibers					142:147	Electrospun starch fibers	123:147	Electrospun starch fibers	123:147	Electrospun starch fibers are promising materials, with potential utility in many fields, including food packaging and tissue engineering.
30925988	1	36	theme	Electrospun	123:133	arg1	materials					163:171	promising materials	153:171	promising materials	153:171	Electrospun starch fibers are promising materials, with potential utility in many fields, including food packaging and tissue engineering.
30925988	1	36	theme	Electrospun	123:133	arg1	packaging					228:236	food packaging	223:236	food packaging	223:236	Electrospun starch fibers are promising materials, with potential utility in many fields, including food packaging and tissue engineering.
30925988	5	37	theme	pure	725:728	arg1	solution					742:749	pure amylopectin solution	725:749	pure amylopectin solution	725:749	All the tested solutions including pure amylopectin solution proved electrospinnable.
30925988	3	38	theme	formic	457:462	arg1	acid					464:467	formic acid	457:467	formic acid	457:467	Various blends of amylose and amylopectin were used to characterize the behavior of starch in formic acid.
30925988	1	39	theme	food	223:226	arg1	packaging					228:236	food packaging	223:236	food packaging	223:236	Electrospun starch fibers are promising materials, with potential utility in many fields, including food packaging and tissue engineering.
30925988	6	40	from	break	854:858	arg1	elongation					840:849	much higher elongation	828:849	much higher elongation at break	828:858	All the fibers were largely amorphous and exhibited much higher elongation at break when compared to cast films of the same composition.
30925988	5	41	theme	amylopectin	730:740	arg1	solution					742:749	pure amylopectin solution	725:749	pure amylopectin solution	725:749	All the tested solutions including pure amylopectin solution proved electrospinnable.
30925988	3	42	theme	amylose	381:387	arg1	blends					371:376	Various blends	363:376	Various blends of amylose and amylopectin	363:403	Various blends of amylose and amylopectin were used to characterize the behavior of starch in formic acid.
30925988	9	43	theme	high	1164:1167	arg1	content					1177:1183	high amylose content	1164:1183	high amylose content	1164:1183	Fibers with high amylose content may have potential application in food packaging or tissue engineering while fibers with high amylopectin concentration are in favor for control drug release.
30925988	9	44	contain	have	1189:1192	arg2	application					1204:1214	potential application	1194:1214	potential application	1194:1214	Fibers with high amylose content may have potential application in food packaging or tissue engineering while fibers with high amylopectin concentration are in favor for control drug release.
30925988	9	44	contain	have	1189:1192	arg1	Fibers					1152:1157	Fibers	1152:1157	Fibers with high amylose content	1152:1183	Fibers with high amylose content may have potential application in food packaging or tissue engineering while fibers with high amylopectin concentration are in favor for control drug release.
30925988	9	45	with	Fibers	1152:1157	arg1	content					1177:1183	high amylose content	1164:1183	high amylose content	1164:1183	Fibers with high amylose content may have potential application in food packaging or tissue engineering while fibers with high amylopectin concentration are in favor for control drug release.
30925988	6	46	theme	cast	877:880	arg1	films					882:886	cast films	877:886	cast films of the same composition	877:910	All the fibers were largely amorphous and exhibited much higher elongation at break when compared to cast films of the same composition.
30925988	9	47	theme	amylose	1169:1175	arg1	content					1177:1183	high amylose content	1164:1183	high amylose content	1164:1183	Fibers with high amylose content may have potential application in food packaging or tissue engineering while fibers with high amylopectin concentration are in favor for control drug release.
30925988	1	48	theme	starch	135:140	arg1	engineering					249:259	tissue engineering	242:259	tissue engineering	242:259	Electrospun starch fibers are promising materials, with potential utility in many fields, including food packaging and tissue engineering.
30925988	1	48	theme	starch	135:140	arg1	fibers					142:147	Electrospun starch fibers	123:147	Electrospun starch fibers	123:147	Electrospun starch fibers are promising materials, with potential utility in many fields, including food packaging and tissue engineering.
30925988	1	48	theme	starch	135:140	arg1	materials					163:171	promising materials	153:171	promising materials	153:171	Electrospun starch fibers are promising materials, with potential utility in many fields, including food packaging and tissue engineering.
30925988	1	48	theme	starch	135:140	arg1	packaging					228:236	food packaging	223:236	food packaging	223:236	Electrospun starch fibers are promising materials, with potential utility in many fields, including food packaging and tissue engineering.
30925988	8	49	theme	types	1132:1136	arg1	application					1107:1117	application	1107:1117	application of these two types of materials	1107:1149	This difference in mechanical properties marks possible fields of application of these two types of materials.
30925988	4	50	theme	ratio	541:545	arg1	effect					511:516	the effect	507:516	the effect of the polysaccharides ratio on phase behavior, rheological properties and spinnability of solutions	507:617	This approach allowed us to focus on the effect of the polysaccharides ratio on phase behavior, rheological properties and spinnability of solutions and eliminate the effect of molecular characteristics of the polymers.
30925988	3	51	used	used	410:413	arg2	blends					371:376	Various blends	363:376	Various blends of amylose and amylopectin	363:403	Various blends of amylose and amylopectin were used to characterize the behavior of starch in formic acid.
30925988	0	52	dep	acid	46:49	arg1	precursor					54:62	precursor	54:62	precursor for electrospun fibers with tunable mechanical properties	54:120	The ternary system amylose-amylopectin-formic acid as precursor for electrospun fibers with tunable mechanical properties.
30925988	8	53	theme	possible	1088:1095	arg1	fields					1097:1102	possible fields	1088:1102	possible fields of application of these two types of materials	1088:1149	This difference in mechanical properties marks possible fields of application of these two types of materials.
30925988	4	54	theme	polysaccharides	525:539	arg1	ratio					541:545	the polysaccharides ratio	521:545	the polysaccharides ratio	521:545	This approach allowed us to focus on the effect of the polysaccharides ratio on phase behavior, rheological properties and spinnability of solutions and eliminate the effect of molecular characteristics of the polymers.
30925988	4	55	theme	solutions	609:617	arg1	spinnability					593:604	spinnability	593:604	spinnability of solutions	593:617	This approach allowed us to focus on the effect of the polysaccharides ratio on phase behavior, rheological properties and spinnability of solutions and eliminate the effect of molecular characteristics of the polymers.
30925988	4	55	theme	solutions	609:617	arg1	behavior					556:563	phase behavior	550:563	phase behavior	550:563	This approach allowed us to focus on the effect of the polysaccharides ratio on phase behavior, rheological properties and spinnability of solutions and eliminate the effect of molecular characteristics of the polymers.
30925988	4	55	theme	solutions	609:617	arg1	properties					578:587	rheological properties	566:587	rheological properties	566:587	This approach allowed us to focus on the effect of the polysaccharides ratio on phase behavior, rheological properties and spinnability of solutions and eliminate the effect of molecular characteristics of the polymers.
30925988	5	56	theme	tested	698:703	arg1	solutions					705:713	All the tested solutions	690:713	All the tested solutions including pure amylopectin solution	690:749	All the tested solutions including pure amylopectin solution proved electrospinnable.
30925988	2	57	theme	present	269:275	arg1	work					277:280	the present work	265:280	the present work	265:280	In the present work, we used formylated derivatives of starch constituents, amylose and amylopectin.
30925988	3	58	from	behavior	435:442	arg1	acid					464:467	formic acid	457:467	formic acid	457:467	Various blends of amylose and amylopectin were used to characterize the behavior of starch in formic acid.
30925988	1	59	theme	promising	153:161	arg1	engineering					249:259	tissue engineering	242:259	tissue engineering	242:259	Electrospun starch fibers are promising materials, with potential utility in many fields, including food packaging and tissue engineering.
30925988	1	59	theme	promising	153:161	arg1	fibers					142:147	Electrospun starch fibers	123:147	Electrospun starch fibers	123:147	Electrospun starch fibers are promising materials, with potential utility in many fields, including food packaging and tissue engineering.
30925988	1	59	theme	promising	153:161	arg1	materials					163:171	promising materials	153:171	promising materials	153:171	Electrospun starch fibers are promising materials, with potential utility in many fields, including food packaging and tissue engineering.
30925988	1	59	theme	promising	153:161	arg1	packaging					228:236	food packaging	223:236	food packaging	223:236	Electrospun starch fibers are promising materials, with potential utility in many fields, including food packaging and tissue engineering.
30925988	1	60	theme	tissue	242:247	arg1	engineering					249:259	tissue engineering	242:259	tissue engineering	242:259	Electrospun starch fibers are promising materials, with potential utility in many fields, including food packaging and tissue engineering.
30925988	8	61	from	difference	1046:1055	arg1	properties					1071:1080	mechanical properties	1060:1080	mechanical properties	1060:1080	This difference in mechanical properties marks possible fields of application of these two types of materials.
30925988	9	62	theme	potential	1194:1202	arg1	application					1204:1214	potential application	1194:1214	potential application	1194:1214	Fibers with high amylose content may have potential application in food packaging or tissue engineering while fibers with high amylopectin concentration are in favor for control drug release.
30925988	0	63	theme	electrospun	68:78	arg1	fibers					80:85	electrospun fibers	68:85	electrospun fibers with tunable mechanical properties	68:120	The ternary system amylose-amylopectin-formic acid as precursor for electrospun fibers with tunable mechanical properties.
30925988	9	64	theme	drug	1330:1333	arg1	release					1335:1341	control drug release	1322:1341	control drug release	1322:1341	Fibers with high amylose content may have potential application in food packaging or tissue engineering while fibers with high amylopectin concentration are in favor for control drug release.
30925988	1	65	from	utility	189:195	arg1	fields					205:210	many fields	200:210	many fields	200:210	Electrospun starch fibers are promising materials, with potential utility in many fields, including food packaging and tissue engineering.
30925988	3	66	theme	amylopectin	393:403	arg1	blends					371:376	Various blends	363:376	Various blends of amylose and amylopectin	363:403	Various blends of amylose and amylopectin were used to characterize the behavior of starch in formic acid.
30574738	0	0	theme	nervous	80:86	arg1	system					88:93	the central nervous system	68:93	the central nervous system	68:93	Does the purinergic system affect extracellular matrix functions in the central nervous system?
30574738	3	1	theme	"	645:645	arg1	gliosis					647:653	" gliosis	645:653	" gliosis	645:653	In response to acute (trauma) or chronic (degenerative) insults, brain ECM modifies its composition and function, actively contributing to "scar forming" gliosis or tissue degeneration/remodelling.
30574738	2	2	theme	tissues	334:340	arg1	components					320:329	all components	316:329	all components of tissues and organs	316:351	It is structurally and functionally present in all components of tissues and organs and represents the substrate on which cells adhere, migrate, proliferate and differentiate, influencing their survival, shape and function.
30574738	3	3	theme	tissue	658:663	arg1	degeneration/remodelling					665:688	tissue degeneration/remodelling	658:688	tissue degeneration/remodelling	658:688	In response to acute (trauma) or chronic (degenerative) insults, brain ECM modifies its composition and function, actively contributing to "scar forming" gliosis or tissue degeneration/remodelling.
30574738	0	4	theme	central	72:78	arg1	system					88:93	the central nervous system	68:93	the central nervous system	68:93	Does the purinergic system affect extracellular matrix functions in the central nervous system?
30574738	4	5	theme	pertinent	837:845	arg1	circuitry					827:835	synaptic circuitry	818:835	synaptic circuitry pertinent to memory formation	818:865	Moreover, morphological changes in dendritic spines associated with extracellular matrix remodeling play key roles in rewiring synaptic circuitry pertinent to memory formation.
30574738	5	6	theme	present	875:881	arg1	report					883:888	the present report	871:888	the present report	871:888	In the present report, we collected the main acquisitions on the functional interplay between ECM alterations and the adenine-/guaninebased purine system with particular regard on how purine compounds and their respective receptors may affect and be affected by changes of the cerebral ECM.
30574738	5	7	theme	respective	1079:1088	arg1	receptors					1090:1098	their respective receptors	1073:1098	their respective receptors	1073:1098	In the present report, we collected the main acquisitions on the functional interplay between ECM alterations and the adenine-/guaninebased purine system with particular regard on how purine compounds and their respective receptors may affect and be affected by changes of the cerebral ECM.
30574738	2	8	from	present	305:311	arg1	components					320:329	all components	316:329	all components of tissues and organs	316:351	It is structurally and functionally present in all components of tissues and organs and represents the substrate on which cells adhere, migrate, proliferate and differentiate, influencing their survival, shape and function.
30574738	3	9	theme	acute	508:512	arg1	insults					549:555	acute (trauma) or chronic (degenerative) insults	508:555	acute (trauma) or chronic (degenerative) insults	508:555	In response to acute (trauma) or chronic (degenerative) insults, brain ECM modifies its composition and function, actively contributing to "scar forming" gliosis or tissue degeneration/remodelling.
30574738	0	10	from	functions	55:63	arg1	system					88:93	the central nervous system	68:93	the central nervous system	68:93	Does the purinergic system affect extracellular matrix functions in the central nervous system?
30574738	4	11	theme	memory	850:855	arg1	formation					857:865	memory formation	850:865	memory formation	850:865	Moreover, morphological changes in dendritic spines associated with extracellular matrix remodeling play key roles in rewiring synaptic circuitry pertinent to memory formation.
30574738	2	12	from	components	320:329	arg1	present					305:311	present	305:311	present	305:311	It is structurally and functionally present in all components of tissues and organs and represents the substrate on which cells adhere, migrate, proliferate and differentiate, influencing their survival, shape and function.
30574738	5	13	theme	functional	933:942	arg1	interplay					944:952	the functional interplay	929:952	the functional interplay between ECM alterations and the adenine-/guaninebased purine system with particular regard	929:1043	In the present report, we collected the main acquisitions on the functional interplay between ECM alterations and the adenine-/guaninebased purine system with particular regard on how purine compounds and their respective receptors may affect and be affected by changes of the cerebral ECM.
30574738	4	14	from	changes	715:721	arg1	spines					736:741	dendritic spines	726:741	dendritic spines associated with extracellular matrix remodeling	726:789	Moreover, morphological changes in dendritic spines associated with extracellular matrix remodeling play key roles in rewiring synaptic circuitry pertinent to memory formation.
30574738	1	15	theme	proteins	148:155	arg1	plethora					136:143	a plethora	134:143	a plethora	134:143	Exracellular matrix (ECM) consists of a plethora of proteins and polysaccharides, which aggregate into an organized network connected to the surface of the producing cells.
30574738	2	16	attach	present	305:311	arg1	components					320:329	all components	316:329	all components of tissues and organs	316:351	It is structurally and functionally present in all components of tissues and organs and represents the substrate on which cells adhere, migrate, proliferate and differentiate, influencing their survival, shape and function.
30574738	2	16	attach	present	305:311	arg2	It					269:270	It	269:270	It	269:270	It is structurally and functionally present in all components of tissues and organs and represents the substrate on which cells adhere, migrate, proliferate and differentiate, influencing their survival, shape and function.
30574738	5	17	with	interplay	944:952	arg1	regard					1038:1043	particular regard	1027:1043	particular regard	1027:1043	In the present report, we collected the main acquisitions on the functional interplay between ECM alterations and the adenine-/guaninebased purine system with particular regard on how purine compounds and their respective receptors may affect and be affected by changes of the cerebral ECM.
30574738	4	18	theme	dendritic	726:734	arg1	spines					736:741	dendritic spines	726:741	dendritic spines associated with extracellular matrix remodeling	726:789	Moreover, morphological changes in dendritic spines associated with extracellular matrix remodeling play key roles in rewiring synaptic circuitry pertinent to memory formation.
30574738	5	19	theme	ECM	1154:1156	arg1	changes					1130:1136	changes	1130:1136	changes of the cerebral ECM	1130:1156	In the present report, we collected the main acquisitions on the functional interplay between ECM alterations and the adenine-/guaninebased purine system with particular regard on how purine compounds and their respective receptors may affect and be affected by changes of the cerebral ECM.
30574738	1	20	theme	polysaccharides	161:175	arg1	plethora					136:143	a plethora	134:143	a plethora	134:143	Exracellular matrix (ECM) consists of a plethora of proteins and polysaccharides, which aggregate into an organized network connected to the surface of the producing cells.
30574738	1	21	theme	producing	252:260	arg1	cells					262:266	the producing cells	248:266	the producing cells	248:266	Exracellular matrix (ECM) consists of a plethora of proteins and polysaccharides, which aggregate into an organized network connected to the surface of the producing cells.
30574738	4	22	theme	synaptic	818:825	arg1	circuitry					827:835	synaptic circuitry	818:835	synaptic circuitry pertinent to memory formation	818:865	Moreover, morphological changes in dendritic spines associated with extracellular matrix remodeling play key roles in rewiring synaptic circuitry pertinent to memory formation.
30574738	3	23	theme	chronic	526:532	arg1	insults					549:555	acute (trauma) or chronic (degenerative) insults	508:555	acute (trauma) or chronic (degenerative) insults	508:555	In response to acute (trauma) or chronic (degenerative) insults, brain ECM modifies its composition and function, actively contributing to "scar forming" gliosis or tissue degeneration/remodelling.
30574738	3	24	dep	insults	549:555	arg1	response					496:503	response	496:503	response	496:503	In response to acute (trauma) or chronic (degenerative) insults, brain ECM modifies its composition and function, actively contributing to "scar forming" gliosis or tissue degeneration/remodelling.
30574738	1	25	theme	cells	262:266	arg1	surface					237:243	the surface	233:243	the surface of the producing cells	233:266	Exracellular matrix (ECM) consists of a plethora of proteins and polysaccharides, which aggregate into an organized network connected to the surface of the producing cells.
30574738	1	26	theme	Exracellular	96:107	arg1	ECM					117:119	ECM	117:119	ECM	117:119	Exracellular matrix (ECM) consists of a plethora of proteins and polysaccharides, which aggregate into an organized network connected to the surface of the producing cells.
30574738	1	26	theme	Exracellular	96:107	arg1	matrix					109:114	Exracellular matrix	96:114	Exracellular matrix (ECM)	96:120	Exracellular matrix (ECM) consists of a plethora of proteins and polysaccharides, which aggregate into an organized network connected to the surface of the producing cells.
30574738	0	27	theme	purinergic	9:18	arg1	system					20:25	the purinergic system	5:25	the purinergic system	5:25	Does the purinergic system affect extracellular matrix functions in the central nervous system?
30574738	3	28	dep	forming	638:644	arg1	scar					633:636	scar	633:636	scar	633:636	In response to acute (trauma) or chronic (degenerative) insults, brain ECM modifies its composition and function, actively contributing to "scar forming" gliosis or tissue degeneration/remodelling.
30574738	5	29	theme	main	908:911	arg1	acquisitions					913:924	the main acquisitions	904:924	the main acquisitions on the functional interplay between ECM alterations and the adenine-/guaninebased purine system with particular regard on how purine compounds and their respective receptors may affect and be affected by changes of the cerebral ECM	904:1156	In the present report, we collected the main acquisitions on the functional interplay between ECM alterations and the adenine-/guaninebased purine system with particular regard on how purine compounds and their respective receptors may affect and be affected by changes of the cerebral ECM.
30574738	5	30	theme	purine	1052:1057	arg1	compounds					1059:1067	purine compounds	1052:1067	purine compounds	1052:1067	In the present report, we collected the main acquisitions on the functional interplay between ECM alterations and the adenine-/guaninebased purine system with particular regard on how purine compounds and their respective receptors may affect and be affected by changes of the cerebral ECM.
30574738	0	31	theme	extracellular	34:46	arg1	functions					55:63	extracellular matrix functions	34:63	extracellular matrix functions in the central nervous system	34:93	Does the purinergic system affect extracellular matrix functions in the central nervous system?
30574738	5	32	theme	adenine-/guaninebased	986:1006	arg1	system					1015:1020	the adenine-/guaninebased purine system	982:1020	the adenine-/guaninebased purine system	982:1020	In the present report, we collected the main acquisitions on the functional interplay between ECM alterations and the adenine-/guaninebased purine system with particular regard on how purine compounds and their respective receptors may affect and be affected by changes of the cerebral ECM.
30574738	5	33	theme	purine	1008:1013	arg1	system					1015:1020	the adenine-/guaninebased purine system	982:1020	the adenine-/guaninebased purine system	982:1020	In the present report, we collected the main acquisitions on the functional interplay between ECM alterations and the adenine-/guaninebased purine system with particular regard on how purine compounds and their respective receptors may affect and be affected by changes of the cerebral ECM.
30574738	5	34	theme	cerebral	1145:1152	arg1	ECM					1154:1156	the cerebral ECM	1141:1156	the cerebral ECM	1141:1156	In the present report, we collected the main acquisitions on the functional interplay between ECM alterations and the adenine-/guaninebased purine system with particular regard on how purine compounds and their respective receptors may affect and be affected by changes of the cerebral ECM.
30574738	2	35	located	present	305:311	arg1	components					320:329	all components	316:329	all components of tissues and organs	316:351	It is structurally and functionally present in all components of tissues and organs and represents the substrate on which cells adhere, migrate, proliferate and differentiate, influencing their survival, shape and function.
30574738	2	35	located	present	305:311	arg2	It					269:270	It	269:270	It	269:270	It is structurally and functionally present in all components of tissues and organs and represents the substrate on which cells adhere, migrate, proliferate and differentiate, influencing their survival, shape and function.
30574738	0	36	theme	matrix	48:53	arg1	functions					55:63	extracellular matrix functions	34:63	extracellular matrix functions in the central nervous system	34:93	Does the purinergic system affect extracellular matrix functions in the central nervous system?
30574738	5	37	theme	particular	1027:1036	arg1	regard					1038:1043	particular regard	1027:1043	particular regard	1027:1043	In the present report, we collected the main acquisitions on the functional interplay between ECM alterations and the adenine-/guaninebased purine system with particular regard on how purine compounds and their respective receptors may affect and be affected by changes of the cerebral ECM.
30574738	4	38	theme	key	796:798	arg1	roles					800:804	key roles	796:804	key roles	796:804	Moreover, morphological changes in dendritic spines associated with extracellular matrix remodeling play key roles in rewiring synaptic circuitry pertinent to memory formation.
30574738	5	39	from	acquisitions	913:924	arg1	interplay					944:952	the functional interplay	929:952	the functional interplay between ECM alterations and the adenine-/guaninebased purine system with particular regard	929:1043	In the present report, we collected the main acquisitions on the functional interplay between ECM alterations and the adenine-/guaninebased purine system with particular regard on how purine compounds and their respective receptors may affect and be affected by changes of the cerebral ECM.
30574738	4	40	theme	extracellular	759:771	arg1	remodeling					780:789	extracellular matrix remodeling	759:789	extracellular matrix remodeling	759:789	Moreover, morphological changes in dendritic spines associated with extracellular matrix remodeling play key roles in rewiring synaptic circuitry pertinent to memory formation.
30574738	5	41	theme	ECM	962:964	arg1	alterations					966:976	ECM alterations	962:976	ECM alterations	962:976	In the present report, we collected the main acquisitions on the functional interplay between ECM alterations and the adenine-/guaninebased purine system with particular regard on how purine compounds and their respective receptors may affect and be affected by changes of the cerebral ECM.
30574738	3	42	dep	acute	508:512	arg1	degenerative					535:546	degenerative	535:546	degenerative	535:546	In response to acute (trauma) or chronic (degenerative) insults, brain ECM modifies its composition and function, actively contributing to "scar forming" gliosis or tissue degeneration/remodelling.
30574738	3	42	dep	acute	508:512	arg1	trauma					515:520	trauma	515:520	trauma	515:520	In response to acute (trauma) or chronic (degenerative) insults, brain ECM modifies its composition and function, actively contributing to "scar forming" gliosis or tissue degeneration/remodelling.
30574738	4	43	theme	morphological	701:713	arg1	changes					715:721	morphological changes	701:721	morphological changes in dendritic spines associated with extracellular matrix remodeling	701:789	Moreover, morphological changes in dendritic spines associated with extracellular matrix remodeling play key roles in rewiring synaptic circuitry pertinent to memory formation.
30574738	1	44	theme	organized	202:210	arg1	network					212:218	an organized network	199:218	an organized network connected to the surface of the producing cells	199:266	Exracellular matrix (ECM) consists of a plethora of proteins and polysaccharides, which aggregate into an organized network connected to the surface of the producing cells.
30574738	4	45	theme	matrix	773:778	arg1	remodeling					780:789	extracellular matrix remodeling	759:789	extracellular matrix remodeling	759:789	Moreover, morphological changes in dendritic spines associated with extracellular matrix remodeling play key roles in rewiring synaptic circuitry pertinent to memory formation.
30574738	2	46	theme	organs	346:351	arg1	components					320:329	all components	316:329	all components of tissues and organs	316:351	It is structurally and functionally present in all components of tissues and organs and represents the substrate on which cells adhere, migrate, proliferate and differentiate, influencing their survival, shape and function.
30574738	3	47	theme	brain	558:562	arg1	ECM					564:566	brain ECM	558:566	brain ECM	558:566	In response to acute (trauma) or chronic (degenerative) insults, brain ECM modifies its composition and function, actively contributing to "scar forming" gliosis or tissue degeneration/remodelling.
30899156	5	0	theme	cheap	307:311	arg1	substrate					313:321	The cheap substrate	303:321	The cheap substrate	303:321	The cheap substrate, wheat bran was used for the production of extracellular polysaccharide in solid state fermentation.
30899156	5	0	theme	cheap	307:311	arg1	bran					330:333	wheat bran	324:333	wheat bran	324:333	The cheap substrate, wheat bran was used for the production of extracellular polysaccharide in solid state fermentation.
30899156	6	1	theme	maltose	453:459	arg1	Supplementation					424:438	Supplementation	424:438	Supplementation of (1%, w/w) maltose, gelatin	424:468	Supplementation of (1%, w/w) maltose, gelatin enhanced EPS production (5.36 mg/g).
30899156	5	2	theme	extracellular	366:378	arg1	polysaccharide					380:393	extracellular polysaccharide	366:393	extracellular polysaccharide	366:393	The cheap substrate, wheat bran was used for the production of extracellular polysaccharide in solid state fermentation.
30899156	9	3	theme	selected	852:859	arg1	factors					861:867	these selected factors	846:867	these selected factors in central composite design	846:895	Among these selected factors in central composite design, maltose significantly influenced on extracellular polysaccharide production.
30899156	4	4	theme	process	235:241	arg1	parameters					243:252	The process parameters	231:252	The process parameters	231:252	The process parameters were initially optimized by traditional methods.
30899156	5	5	theme	polysaccharide	380:393	arg1	production					352:361	the production	348:361	the production of extracellular polysaccharide in solid state fermentation	348:421	The cheap substrate, wheat bran was used for the production of extracellular polysaccharide in solid state fermentation.
30899156	3	6	theme	polysaccharide	215:228	arg1	production					187:196	the production	183:196	the production of extracellular polysaccharide	183:228	was isolated for the production of extracellular polysaccharide.
30899156	8	7	theme	various	761:767	arg1	combinations					769:780	various combinations	761:780	various combinations of the components supplemented with wheat bran substrate	761:837	In two level full factorial experimental designs, the EPS yield varied from 3.18 to 11.65 mg/g wheat bran substrate with various combinations of the components supplemented with wheat bran substrate.
30899156	5	8	theme	solid	398:402	arg1	fermentation					410:421	solid state fermentation	398:421	solid state fermentation	398:421	The cheap substrate, wheat bran was used for the production of extracellular polysaccharide in solid state fermentation.
30899156	6	9	dep	maltose	453:459	arg1	w/w					448:450	w/w	448:450	w/w	448:450	Supplementation of (1%, w/w) maltose, gelatin enhanced EPS production (5.36 mg/g).
30899156	6	9	dep	maltose	453:459	arg1	%					445:445	1%	444:445	1%	444:445	Supplementation of (1%, w/w) maltose, gelatin enhanced EPS production (5.36 mg/g).
30899156	2	10	theme	Aspergillus	150:160	arg1	sp					162:163	Aspergillus sp	150:163	Aspergillus sp	150:163	In this study, Aspergillus sp.
30899156	5	11	from	production	352:361	arg1	fermentation					410:421	solid state fermentation	398:421	solid state fermentation	398:421	The cheap substrate, wheat bran was used for the production of extracellular polysaccharide in solid state fermentation.
30899156	9	12	theme	composite	880:888	arg1	design					890:895	central composite design	872:895	central composite design	872:895	Among these selected factors in central composite design, maltose significantly influenced on extracellular polysaccharide production.
30899156	9	13	theme	central	872:878	arg1	design					890:895	central composite design	872:895	central composite design	872:895	Among these selected factors in central composite design, maltose significantly influenced on extracellular polysaccharide production.
30899156	1	14	theme	extracellular	72:84	arg1	polysaccharides					86:100	extracellular polysaccharides	72:100	extracellular polysaccharides	72:100	for the production of extracellular polysaccharides by response surface methodology.
30899156	8	15	theme	experimental	668:679	arg1	designs					681:687	two level full factorial experimental designs	643:687	two level full factorial experimental designs	643:687	In two level full factorial experimental designs, the EPS yield varied from 3.18 to 11.65 mg/g wheat bran substrate with various combinations of the components supplemented with wheat bran substrate.
30899156	3	16	theme	extracellular	201:213	arg1	polysaccharide					215:228	extracellular polysaccharide	201:228	extracellular polysaccharide	201:228	was isolated for the production of extracellular polysaccharide.
30899156	1	17	theme	polysaccharides	86:100	arg1	production					58:67	the production	54:67	the production of extracellular polysaccharides by response surface methodology	54:132	for the production of extracellular polysaccharides by response surface methodology.
30899156	8	18	theme	components	789:798	arg1	combinations					769:780	various combinations	761:780	various combinations of the components supplemented with wheat bran substrate	761:837	In two level full factorial experimental designs, the EPS yield varied from 3.18 to 11.65 mg/g wheat bran substrate with various combinations of the components supplemented with wheat bran substrate.
30899156	4	19	theme	traditional	282:292	arg1	methods					294:300	traditional methods	282:300	traditional methods	282:300	The process parameters were initially optimized by traditional methods.
30899156	1	20	theme	response	105:112	arg1	methodology					122:132	response surface methodology	105:132	response surface methodology	105:132	for the production of extracellular polysaccharides by response surface methodology.
30899156	2	21	dep	sp	162:163	arg1	study					143:147	this study	138:147	this study	138:147	In this study, Aspergillus sp.
30899156	5	22	used	used	339:342	arg2	substrate					313:321	The cheap substrate	303:321	The cheap substrate	303:321	The cheap substrate, wheat bran was used for the production of extracellular polysaccharide in solid state fermentation.
30899156	5	22	used	used	339:342	arg2	bran					330:333	wheat bran	324:333	wheat bran	324:333	The cheap substrate, wheat bran was used for the production of extracellular polysaccharide in solid state fermentation.
30899156	1	23	theme	surface	114:120	arg1	methodology					122:132	response surface methodology	105:132	response surface methodology	105:132	for the production of extracellular polysaccharides by response surface methodology.
30899156	8	24	theme	wheat	818:822	arg1	bran					824:827	wheat bran	818:827	wheat bran substrate	818:837	In two level full factorial experimental designs, the EPS yield varied from 3.18 to 11.65 mg/g wheat bran substrate with various combinations of the components supplemented with wheat bran substrate.
30899156	0	25	theme	sp	46:47	arg1	characterization					14:29	characterization	14:29	characterization	14:29	Isolation and characterization of Aspergillus sp.
30899156	0	25	theme	sp	46:47	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation and characterization of Aspergillus sp.
30899156	7	26	theme	EPS	624:626	arg1	production					628:637	EPS production	624:637	EPS production	624:637	The salts such as, Cu2+ (4.9 mg/g), Ca2+ (3.5 mg/g), Zn2+ (2.9 mg/g), Mn2+ (3.4 mg/g) and Mg2+ (1.8 mg/g) stimulated EPS production.
30899156	8	27	theme	bran	824:827	arg1	substrate					829:837	wheat bran substrate	818:837	wheat bran substrate	818:837	In two level full factorial experimental designs, the EPS yield varied from 3.18 to 11.65 mg/g wheat bran substrate with various combinations of the components supplemented with wheat bran substrate.
30899156	0	28	theme	Aspergillus	34:44	arg1	sp					46:47	Aspergillus sp	34:47	Aspergillus sp	34:47	Isolation and characterization of Aspergillus sp.
30899156	5	29	theme	wheat	324:328	arg1	substrate					313:321	The cheap substrate	303:321	The cheap substrate	303:321	The cheap substrate, wheat bran was used for the production of extracellular polysaccharide in solid state fermentation.
30899156	5	29	theme	wheat	324:328	arg1	bran					330:333	wheat bran	324:333	wheat bran	324:333	The cheap substrate, wheat bran was used for the production of extracellular polysaccharide in solid state fermentation.
30899156	9	30	from	factors	861:867	arg1	design					890:895	central composite design	872:895	central composite design	872:895	Among these selected factors in central composite design, maltose significantly influenced on extracellular polysaccharide production.
30899156	9	31	theme	extracellular	934:946	arg1	production					963:972	extracellular polysaccharide production	934:972	extracellular polysaccharide production	934:972	Among these selected factors in central composite design, maltose significantly influenced on extracellular polysaccharide production.
30899156	8	32	theme	level	647:651	arg1	designs					681:687	two level full factorial experimental designs	643:687	two level full factorial experimental designs	643:687	In two level full factorial experimental designs, the EPS yield varied from 3.18 to 11.65 mg/g wheat bran substrate with various combinations of the components supplemented with wheat bran substrate.
30899156	6	33	theme	EPS	479:481	arg1	5.36 mg/g					495:503	5.36 mg/g	495:503	5.36 mg/g	495:503	Supplementation of (1%, w/w) maltose, gelatin enhanced EPS production (5.36 mg/g).
30899156	6	33	theme	EPS	479:481	arg1	production					483:492	EPS production	479:492	EPS production (5.36 mg/g)	479:504	Supplementation of (1%, w/w) maltose, gelatin enhanced EPS production (5.36 mg/g).
30899156	9	34	theme	polysaccharide	948:961	arg1	production					963:972	extracellular polysaccharide production	934:972	extracellular polysaccharide production	934:972	Among these selected factors in central composite design, maltose significantly influenced on extracellular polysaccharide production.
30899156	8	35	theme	full	653:656	arg1	designs					681:687	two level full factorial experimental designs	643:687	two level full factorial experimental designs	643:687	In two level full factorial experimental designs, the EPS yield varied from 3.18 to 11.65 mg/g wheat bran substrate with various combinations of the components supplemented with wheat bran substrate.
30899156	8	36	theme	11.65 mg/g	724:733	arg1	bran					741:744	11.65 mg/g wheat bran	724:744	11.65 mg/g wheat bran substrate	724:754	In two level full factorial experimental designs, the EPS yield varied from 3.18 to 11.65 mg/g wheat bran substrate with various combinations of the components supplemented with wheat bran substrate.
30899156	8	37	theme	factorial	658:666	arg1	designs					681:687	two level full factorial experimental designs	643:687	two level full factorial experimental designs	643:687	In two level full factorial experimental designs, the EPS yield varied from 3.18 to 11.65 mg/g wheat bran substrate with various combinations of the components supplemented with wheat bran substrate.
30899156	5	38	theme	state	404:408	arg1	fermentation					410:421	solid state fermentation	398:421	solid state fermentation	398:421	The cheap substrate, wheat bran was used for the production of extracellular polysaccharide in solid state fermentation.
30899156	8	39	theme	bran	741:744	arg1	substrate					746:754	11.65 mg/g wheat bran substrate	724:754	11.65 mg/g wheat bran substrate	724:754	In two level full factorial experimental designs, the EPS yield varied from 3.18 to 11.65 mg/g wheat bran substrate with various combinations of the components supplemented with wheat bran substrate.
30899156	8	40	theme	wheat	735:739	arg1	bran					741:744	11.65 mg/g wheat bran	724:744	11.65 mg/g wheat bran substrate	724:754	In two level full factorial experimental designs, the EPS yield varied from 3.18 to 11.65 mg/g wheat bran substrate with various combinations of the components supplemented with wheat bran substrate.
30899156	8	41	theme	EPS	694:696	arg1	yield					698:702	the EPS yield	690:702	the EPS yield	690:702	In two level full factorial experimental designs, the EPS yield varied from 3.18 to 11.65 mg/g wheat bran substrate with various combinations of the components supplemented with wheat bran substrate.
29945790	4	0	theme	typical	845:851	arg1	simple					878:883	simple	878:883	simple	878:883	A typical LDIS-CE-LIF protocol was simple: a short-plug of leading electrolyte (LE) and large-volume sample solution were introduced to a capillary, followed by application of constant voltage.
29945790	4	0	theme	typical	845:851	arg1	protocol					865:872	A typical LDIS-CE-LIF protocol	843:872	A typical LDIS-CE-LIF protocol	843:872	A typical LDIS-CE-LIF protocol was simple: a short-plug of leading electrolyte (LE) and large-volume sample solution were introduced to a capillary, followed by application of constant voltage.
29945790	10	1	theme	LDIS-CE-LIF	1864:1874	arg1	potential					1851:1859	the potential	1847:1859	the potential of LDIS-CE-LIF for discovery of disease-specific N-linked glycans in microscale environment	1847:1951	It suggests the potential of LDIS-CE-LIF for discovery of disease-specific N-linked glycans in microscale environment.
29945790	10	2	link	N-linked	1910:1917	arg1	glycans					1919:1925	disease-specific N-linked glycans	1893:1925	disease-specific N-linked glycans	1893:1925	It suggests the potential of LDIS-CE-LIF for discovery of disease-specific N-linked glycans in microscale environment.
29945790	3	3	link	N-linked	487:494	arg1	glycans					496:502	N-linked glycans	487:502	N-linked glycans	487:502	For analyzing tiny biological samples, we developed sensitive, high-resolution, and salt-tolerant method for analyzing trace level of N-linked glycans by coupling capillary electrophoresis (CE), laser-induced fluorescence (LIF) detection, and a new online sample preconcentration (OSP) method named "large-volume dual preconcentration by isotachophoresis and stacking (LDIS)", which is composed of two OSP methods, large-volume sample stacking (LVSS) and transient isotachophoresis (tITP).
29945790	0	4	link	N-linked	13:20	arg1	glycans					22:28	N-linked glycans	13:28	N-linked glycans from 100 cells	13:43	Profiling of N-linked glycans from 100 cells by capillary electrophoresis with large-volume dual preconcentration by isotachophoresis and stacking.
29945790	4	5	theme	voltage	1028:1034	arg1	application					1004:1014	application	1004:1014	application of constant voltage	1004:1034	A typical LDIS-CE-LIF protocol was simple: a short-plug of leading electrolyte (LE) and large-volume sample solution were introduced to a capillary, followed by application of constant voltage.
29945790	10	6	theme	glycans	1919:1925	arg1	discovery					1880:1888	discovery	1880:1888	discovery of disease-specific N-linked glycans in microscale environment	1880:1951	It suggests the potential of LDIS-CE-LIF for discovery of disease-specific N-linked glycans in microscale environment.
29945790	4	7	dep	introduced	965:974	arg1	followed					992:999	followed	992:999	followed by application of constant voltage	992:1034	A typical LDIS-CE-LIF protocol was simple: a short-plug of leading electrolyte (LE) and large-volume sample solution were introduced to a capillary, followed by application of constant voltage.
29945790	0	8	with	electrophoresis	58:72	arg1	preconcentration					97:112	large-volume dual preconcentration	79:112	large-volume dual preconcentration by isotachophoresis and stacking	79:145	Profiling of N-linked glycans from 100 cells by capillary electrophoresis with large-volume dual preconcentration by isotachophoresis and stacking.
29945790	3	9	theme	LIF	576:578	arg1	detection					581:589	laser-induced fluorescence (LIF) detection	548:589	laser-induced fluorescence (LIF) detection	548:589	For analyzing tiny biological samples, we developed sensitive, high-resolution, and salt-tolerant method for analyzing trace level of N-linked glycans by coupling capillary electrophoresis (CE), laser-induced fluorescence (LIF) detection, and a new online sample preconcentration (OSP) method named "large-volume dual preconcentration by isotachophoresis and stacking (LDIS)", which is composed of two OSP methods, large-volume sample stacking (LVSS) and transient isotachophoresis (tITP).
29945790	6	10	dep	pressure	1265:1272	arg1	assist					1274:1279	assist	1274:1279	assist	1274:1279	By applying pressure assist during preconcentration, both viscous gel electrolyte and salty matrix of up to 10 mM NaCl were acceptable.
29945790	9	11	theme	N-glycan	1611:1618	arg1	structure					1620:1628	N-glycan structure	1611:1628	N-glycan structure	1611:1628	N-glycan structure could be predicted by searching glucose-unit value via Glycobase database, indicating that HepG2 expressed more sialylated glycans and MCF-7 expressed less glycans respectively, comparing with HeLa cells.
29945790	10	12	theme	disease-specific	1893:1908	arg1	glycans					1919:1925	disease-specific N-linked glycans	1893:1925	disease-specific N-linked glycans	1893:1925	It suggests the potential of LDIS-CE-LIF for discovery of disease-specific N-linked glycans in microscale environment.
29945790	3	13	theme	fluorescence	562:573	arg1	detection					581:589	laser-induced fluorescence (LIF) detection	548:589	laser-induced fluorescence (LIF) detection	548:589	For analyzing tiny biological samples, we developed sensitive, high-resolution, and salt-tolerant method for analyzing trace level of N-linked glycans by coupling capillary electrophoresis (CE), laser-induced fluorescence (LIF) detection, and a new online sample preconcentration (OSP) method named "large-volume dual preconcentration by isotachophoresis and stacking (LDIS)", which is composed of two OSP methods, large-volume sample stacking (LVSS) and transient isotachophoresis (tITP).
29945790	10	14	from	discovery	1880:1888	arg1	environment					1941:1951	microscale environment	1930:1951	microscale environment	1930:1951	It suggests the potential of LDIS-CE-LIF for discovery of disease-specific N-linked glycans in microscale environment.
29945790	2	15	theme	glycans	232:238	arg1	Profiling					219:227	Profiling	219:227	Profiling of glycans from limited number of pathogenetic cells in an early-stage tissue	219:305	Profiling of glycans from limited number of pathogenetic cells in an early-stage tissue is essential for discovering effective drugs.
29945790	10	16	theme	microscale	1930:1939	arg1	environment					1941:1951	microscale environment	1930:1951	microscale environment	1930:1951	It suggests the potential of LDIS-CE-LIF for discovery of disease-specific N-linked glycans in microscale environment.
29945790	6	17	theme	gel	1319:1321	arg1	electrolyte					1323:1333	viscous gel electrolyte	1311:1333	viscous gel electrolyte	1311:1333	By applying pressure assist during preconcentration, both viscous gel electrolyte and salty matrix of up to 10 mM NaCl were acceptable.
29945790	8	18	dep	40	1525:1526	arg1	to					1522:1523	to	1522:1523	to	1522:1523	From 30 to 40 glycans were successfully detected with almost same profile of large-scale sample.
29945790	4	19	theme	electrolyte	910:920	arg1	short-plug					888:897	a short-plug	886:897	a short-plug of leading electrolyte (LE) and large-volume sample solution	886:958	A typical LDIS-CE-LIF protocol was simple: a short-plug of leading electrolyte (LE) and large-volume sample solution were introduced to a capillary, followed by application of constant voltage.
29945790	2	20	theme	limited	245:251	arg1	number					253:258	limited number	245:258	limited number of pathogenetic cells	245:280	Profiling of glycans from limited number of pathogenetic cells in an early-stage tissue is essential for discovering effective drugs.
29945790	9	21	theme	sialylated	1742:1751	arg1	glycans					1753:1759	expressed more sialylated glycans	1727:1759	expressed more sialylated glycans	1727:1759	N-glycan structure could be predicted by searching glucose-unit value via Glycobase database, indicating that HepG2 expressed more sialylated glycans and MCF-7 expressed less glycans respectively, comparing with HeLa cells.
29945790	3	22	theme	OSP	755:757	arg1	methods					759:765	two OSP methods	751:765	two OSP methods	751:765	For analyzing tiny biological samples, we developed sensitive, high-resolution, and salt-tolerant method for analyzing trace level of N-linked glycans by coupling capillary electrophoresis (CE), laser-induced fluorescence (LIF) detection, and a new online sample preconcentration (OSP) method named "large-volume dual preconcentration by isotachophoresis and stacking (LDIS)", which is composed of two OSP methods, large-volume sample stacking (LVSS) and transient isotachophoresis (tITP).
29945790	3	22	theme	OSP	755:757	arg1	stacking					788:795	large-volume sample stacking	768:795	large-volume sample stacking (LVSS)	768:802	For analyzing tiny biological samples, we developed sensitive, high-resolution, and salt-tolerant method for analyzing trace level of N-linked glycans by coupling capillary electrophoresis (CE), laser-induced fluorescence (LIF) detection, and a new online sample preconcentration (OSP) method named "large-volume dual preconcentration by isotachophoresis and stacking (LDIS)", which is composed of two OSP methods, large-volume sample stacking (LVSS) and transient isotachophoresis (tITP).
29945790	3	22	theme	OSP	755:757	arg1	isotachophoresis					818:833	transient isotachophoresis	808:833	transient isotachophoresis	808:833	For analyzing tiny biological samples, we developed sensitive, high-resolution, and salt-tolerant method for analyzing trace level of N-linked glycans by coupling capillary electrophoresis (CE), laser-induced fluorescence (LIF) detection, and a new online sample preconcentration (OSP) method named "large-volume dual preconcentration by isotachophoresis and stacking (LDIS)", which is composed of two OSP methods, large-volume sample stacking (LVSS) and transient isotachophoresis (tITP).
29945790	3	23	theme	new	598:600	arg1	method					639:644	a new online sample preconcentration (OSP) method	596:644	a new online sample preconcentration (OSP) method named "large-volume dual preconcentration by isotachophoresis and stacking (LDIS)", which is composed of two OSP methods, large-volume sample stacking (LVSS) and transient isotachophoresis (tITP)	596:840	For analyzing tiny biological samples, we developed sensitive, high-resolution, and salt-tolerant method for analyzing trace level of N-linked glycans by coupling capillary electrophoresis (CE), laser-induced fluorescence (LIF) detection, and a new online sample preconcentration (OSP) method named "large-volume dual preconcentration by isotachophoresis and stacking (LDIS)", which is composed of two OSP methods, large-volume sample stacking (LVSS) and transient isotachophoresis (tITP).
29945790	9	24	theme	Glycobase	1685:1693	arg1	database					1695:1702	Glycobase database	1685:1702	Glycobase database	1685:1702	N-glycan structure could be predicted by searching glucose-unit value via Glycobase database, indicating that HepG2 expressed more sialylated glycans and MCF-7 expressed less glycans respectively, comparing with HeLa cells.
29945790	2	25	theme	cells	276:280	arg1	number					253:258	limited number	245:258	limited number of pathogenetic cells	245:280	Profiling of glycans from limited number of pathogenetic cells in an early-stage tissue is essential for discovering effective drugs.
29945790	1	26	theme	Glycan	148:153	arg1	structure					155:163	Glycan structure	148:163	Glycan structure	148:163	Glycan structure is changed in response with pathogenesis like cancer.
29945790	4	27	theme	sample	944:949	arg1	solution					951:958	large-volume sample solution	931:958	large-volume sample solution	931:958	A typical LDIS-CE-LIF protocol was simple: a short-plug of leading electrolyte (LE) and large-volume sample solution were introduced to a capillary, followed by application of constant voltage.
29945790	3	28	theme	sample	781:786	arg1	methods					759:765	two OSP methods	751:765	two OSP methods	751:765	For analyzing tiny biological samples, we developed sensitive, high-resolution, and salt-tolerant method for analyzing trace level of N-linked glycans by coupling capillary electrophoresis (CE), laser-induced fluorescence (LIF) detection, and a new online sample preconcentration (OSP) method named "large-volume dual preconcentration by isotachophoresis and stacking (LDIS)", which is composed of two OSP methods, large-volume sample stacking (LVSS) and transient isotachophoresis (tITP).
29945790	3	28	theme	sample	781:786	arg1	LVSS					798:801	LVSS	798:801	LVSS	798:801	For analyzing tiny biological samples, we developed sensitive, high-resolution, and salt-tolerant method for analyzing trace level of N-linked glycans by coupling capillary electrophoresis (CE), laser-induced fluorescence (LIF) detection, and a new online sample preconcentration (OSP) method named "large-volume dual preconcentration by isotachophoresis and stacking (LDIS)", which is composed of two OSP methods, large-volume sample stacking (LVSS) and transient isotachophoresis (tITP).
29945790	3	28	theme	sample	781:786	arg1	stacking					788:795	large-volume sample stacking	768:795	large-volume sample stacking (LVSS)	768:802	For analyzing tiny biological samples, we developed sensitive, high-resolution, and salt-tolerant method for analyzing trace level of N-linked glycans by coupling capillary electrophoresis (CE), laser-induced fluorescence (LIF) detection, and a new online sample preconcentration (OSP) method named "large-volume dual preconcentration by isotachophoresis and stacking (LDIS)", which is composed of two OSP methods, large-volume sample stacking (LVSS) and transient isotachophoresis (tITP).
29945790	3	28	theme	sample	781:786	arg1	tITP					836:839	tITP	836:839	tITP	836:839	For analyzing tiny biological samples, we developed sensitive, high-resolution, and salt-tolerant method for analyzing trace level of N-linked glycans by coupling capillary electrophoresis (CE), laser-induced fluorescence (LIF) detection, and a new online sample preconcentration (OSP) method named "large-volume dual preconcentration by isotachophoresis and stacking (LDIS)", which is composed of two OSP methods, large-volume sample stacking (LVSS) and transient isotachophoresis (tITP).
29945790	0	29	theme	capillary	48:56	arg1	electrophoresis					58:72	capillary electrophoresis	48:72	capillary electrophoresis with large-volume dual preconcentration by isotachophoresis and stacking	48:145	Profiling of N-linked glycans from 100 cells by capillary electrophoresis with large-volume dual preconcentration by isotachophoresis and stacking.
29945790	5	30	theme	8-aminopyrene-1,3,6-trisulfonic	1084:1114	arg1	acid					1116:1119	8-aminopyrene-1,3,6-trisulfonic acid	1084:1119	8-aminopyrene-1,3,6-trisulfonic acid	1084:1119	In the analysis of glucose ladder labeled with 8-aminopyrene-1,3,6-trisulfonic acid with 10 mM sodium chloride as LE, up to 2300-fold sensitivity increase was achieved with higher resolution than those in normal CE.
29945790	5	31	dep	2300-fold	1161:1169	arg1	to					1158:1159	to	1158:1159	to	1158:1159	In the analysis of glucose ladder labeled with 8-aminopyrene-1,3,6-trisulfonic acid with 10 mM sodium chloride as LE, up to 2300-fold sensitivity increase was achieved with higher resolution than those in normal CE.
29945790	3	32	theme	transient	808:816	arg1	methods					759:765	two OSP methods	751:765	two OSP methods	751:765	For analyzing tiny biological samples, we developed sensitive, high-resolution, and salt-tolerant method for analyzing trace level of N-linked glycans by coupling capillary electrophoresis (CE), laser-induced fluorescence (LIF) detection, and a new online sample preconcentration (OSP) method named "large-volume dual preconcentration by isotachophoresis and stacking (LDIS)", which is composed of two OSP methods, large-volume sample stacking (LVSS) and transient isotachophoresis (tITP).
29945790	3	32	theme	transient	808:816	arg1	isotachophoresis					818:833	transient isotachophoresis	808:833	transient isotachophoresis	808:833	For analyzing tiny biological samples, we developed sensitive, high-resolution, and salt-tolerant method for analyzing trace level of N-linked glycans by coupling capillary electrophoresis (CE), laser-induced fluorescence (LIF) detection, and a new online sample preconcentration (OSP) method named "large-volume dual preconcentration by isotachophoresis and stacking (LDIS)", which is composed of two OSP methods, large-volume sample stacking (LVSS) and transient isotachophoresis (tITP).
29945790	9	33	theme	expressed	1727:1735	arg1	glycans					1753:1759	expressed more sialylated glycans	1727:1759	expressed more sialylated glycans	1727:1759	N-glycan structure could be predicted by searching glucose-unit value via Glycobase database, indicating that HepG2 expressed more sialylated glycans and MCF-7 expressed less glycans respectively, comparing with HeLa cells.
29945790	0	34	from	Profiling	0:8	arg1	cells					39:43	100 cells	35:43	100 cells	35:43	Profiling of N-linked glycans from 100 cells by capillary electrophoresis with large-volume dual preconcentration by isotachophoresis and stacking.
29945790	0	35	theme	dual	92:95	arg1	preconcentration					97:112	large-volume dual preconcentration	79:112	large-volume dual preconcentration by isotachophoresis and stacking	79:145	Profiling of N-linked glycans from 100 cells by capillary electrophoresis with large-volume dual preconcentration by isotachophoresis and stacking.
29945790	5	36	theme	glucose	1056:1062	arg1	ladder					1064:1069	glucose ladder	1056:1069	glucose ladder	1056:1069	In the analysis of glucose ladder labeled with 8-aminopyrene-1,3,6-trisulfonic acid with 10 mM sodium chloride as LE, up to 2300-fold sensitivity increase was achieved with higher resolution than those in normal CE.
29945790	7	37	theme	tumor	1501:1505	arg1	cells					1507:1511	localized tumor cells	1491:1511	localized tumor cells	1491:1511	Finally, N-glycans from approximately 100 cells (HeLa, MCF7, and HepG2) were analyzed as the model of localized tumor cells.
29945790	3	38	theme	tiny	367:370	arg1	samples					383:389	tiny biological samples	367:389	tiny biological samples	367:389	For analyzing tiny biological samples, we developed sensitive, high-resolution, and salt-tolerant method for analyzing trace level of N-linked glycans by coupling capillary electrophoresis (CE), laser-induced fluorescence (LIF) detection, and a new online sample preconcentration (OSP) method named "large-volume dual preconcentration by isotachophoresis and stacking (LDIS)", which is composed of two OSP methods, large-volume sample stacking (LVSS) and transient isotachophoresis (tITP).
29945790	0	39	from	cells	39:43	arg1	Profiling					0:8	Profiling	0:8	Profiling of N-linked glycans from 100 cells by capillary electrophoresis with large-volume dual preconcentration by isotachophoresis and stacking.	0:146	Profiling of N-linked glycans from 100 cells by capillary electrophoresis with large-volume dual preconcentration by isotachophoresis and stacking.
29945790	0	39	from	cells	39:43	arg1	glycans					22:28	N-linked glycans	13:28	N-linked glycans from 100 cells	13:43	Profiling of N-linked glycans from 100 cells by capillary electrophoresis with large-volume dual preconcentration by isotachophoresis and stacking.
29945790	2	40	from	number	253:258	arg1	glycans					232:238	glycans	232:238	glycans from limited number of pathogenetic cells	232:280	Profiling of glycans from limited number of pathogenetic cells in an early-stage tissue is essential for discovering effective drugs.
29945790	2	40	from	number	253:258	arg1	Profiling					219:227	Profiling	219:227	Profiling of glycans from limited number of pathogenetic cells in an early-stage tissue	219:305	Profiling of glycans from limited number of pathogenetic cells in an early-stage tissue is essential for discovering effective drugs.
29945790	3	41	theme	sensitive	405:413	arg1	method					451:456	sensitive, high-resolution, and salt-tolerant method	405:456	sensitive, high-resolution, and salt-tolerant method for analyzing trace level of N-linked glycans by coupling capillary electrophoresis (CE), laser-induced fluorescence (LIF) detection, and a new online sample preconcentration (OSP) method named "large-volume dual preconcentration by isotachophoresis and stacking (LDIS)", which is composed of two OSP methods, large-volume sample stacking (LVSS) and transient isotachophoresis (tITP)	405:840	For analyzing tiny biological samples, we developed sensitive, high-resolution, and salt-tolerant method for analyzing trace level of N-linked glycans by coupling capillary electrophoresis (CE), laser-induced fluorescence (LIF) detection, and a new online sample preconcentration (OSP) method named "large-volume dual preconcentration by isotachophoresis and stacking (LDIS)", which is composed of two OSP methods, large-volume sample stacking (LVSS) and transient isotachophoresis (tITP).
29945790	6	42	dep	10 mM	1361:1365	arg1	to					1358:1359	to	1358:1359	to	1358:1359	By applying pressure assist during preconcentration, both viscous gel electrolyte and salty matrix of up to 10 mM NaCl were acceptable.
29945790	2	43	theme	pathogenetic	263:274	arg1	cells					276:280	pathogenetic cells	263:280	pathogenetic cells	263:280	Profiling of glycans from limited number of pathogenetic cells in an early-stage tissue is essential for discovering effective drugs.
29945790	3	44	theme	N-linked	487:494	arg1	glycans					496:502	N-linked glycans	487:502	N-linked glycans	487:502	For analyzing tiny biological samples, we developed sensitive, high-resolution, and salt-tolerant method for analyzing trace level of N-linked glycans by coupling capillary electrophoresis (CE), laser-induced fluorescence (LIF) detection, and a new online sample preconcentration (OSP) method named "large-volume dual preconcentration by isotachophoresis and stacking (LDIS)", which is composed of two OSP methods, large-volume sample stacking (LVSS) and transient isotachophoresis (tITP).
29945790	0	45	theme	N-linked	13:20	arg1	glycans					22:28	N-linked glycans	13:28	N-linked glycans from 100 cells	13:43	Profiling of N-linked glycans from 100 cells by capillary electrophoresis with large-volume dual preconcentration by isotachophoresis and stacking.
29945790	3	46	theme	laser-induced	548:560	arg1	detection					581:589	laser-induced fluorescence (LIF) detection	548:589	laser-induced fluorescence (LIF) detection	548:589	For analyzing tiny biological samples, we developed sensitive, high-resolution, and salt-tolerant method for analyzing trace level of N-linked glycans by coupling capillary electrophoresis (CE), laser-induced fluorescence (LIF) detection, and a new online sample preconcentration (OSP) method named "large-volume dual preconcentration by isotachophoresis and stacking (LDIS)", which is composed of two OSP methods, large-volume sample stacking (LVSS) and transient isotachophoresis (tITP).
29945790	3	47	theme	large-volume	653:664	arg1	preconcentration					671:686	"large-volume dual preconcentration	652:686	"large-volume dual preconcentration	652:686	For analyzing tiny biological samples, we developed sensitive, high-resolution, and salt-tolerant method for analyzing trace level of N-linked glycans by coupling capillary electrophoresis (CE), laser-induced fluorescence (LIF) detection, and a new online sample preconcentration (OSP) method named "large-volume dual preconcentration by isotachophoresis and stacking (LDIS)", which is composed of two OSP methods, large-volume sample stacking (LVSS) and transient isotachophoresis (tITP).
29945790	1	48	dep	pathogenesis	193:204	arg1	response					179:186	response	179:186	response	179:186	Glycan structure is changed in response with pathogenesis like cancer.
29945790	5	49	theme	sodium	1132:1137	arg1	chloride					1139:1146	10 mM sodium chloride	1126:1146	10 mM sodium chloride	1126:1146	In the analysis of glucose ladder labeled with 8-aminopyrene-1,3,6-trisulfonic acid with 10 mM sodium chloride as LE, up to 2300-fold sensitivity increase was achieved with higher resolution than those in normal CE.
29945790	3	50	theme	high-resolution	416:430	arg1	method					451:456	sensitive, high-resolution, and salt-tolerant method	405:456	sensitive, high-resolution, and salt-tolerant method for analyzing trace level of N-linked glycans by coupling capillary electrophoresis (CE), laser-induced fluorescence (LIF) detection, and a new online sample preconcentration (OSP) method named "large-volume dual preconcentration by isotachophoresis and stacking (LDIS)", which is composed of two OSP methods, large-volume sample stacking (LVSS) and transient isotachophoresis (tITP)	405:840	For analyzing tiny biological samples, we developed sensitive, high-resolution, and salt-tolerant method for analyzing trace level of N-linked glycans by coupling capillary electrophoresis (CE), laser-induced fluorescence (LIF) detection, and a new online sample preconcentration (OSP) method named "large-volume dual preconcentration by isotachophoresis and stacking (LDIS)", which is composed of two OSP methods, large-volume sample stacking (LVSS) and transient isotachophoresis (tITP).
29945790	3	51	theme	capillary	516:524	arg1	CE					543:544	CE	543:544	CE	543:544	For analyzing tiny biological samples, we developed sensitive, high-resolution, and salt-tolerant method for analyzing trace level of N-linked glycans by coupling capillary electrophoresis (CE), laser-induced fluorescence (LIF) detection, and a new online sample preconcentration (OSP) method named "large-volume dual preconcentration by isotachophoresis and stacking (LDIS)", which is composed of two OSP methods, large-volume sample stacking (LVSS) and transient isotachophoresis (tITP).
29945790	3	51	theme	capillary	516:524	arg1	electrophoresis					526:540	coupling capillary electrophoresis	507:540	coupling capillary electrophoresis (CE)	507:545	For analyzing tiny biological samples, we developed sensitive, high-resolution, and salt-tolerant method for analyzing trace level of N-linked glycans by coupling capillary electrophoresis (CE), laser-induced fluorescence (LIF) detection, and a new online sample preconcentration (OSP) method named "large-volume dual preconcentration by isotachophoresis and stacking (LDIS)", which is composed of two OSP methods, large-volume sample stacking (LVSS) and transient isotachophoresis (tITP).
29945790	8	52	theme	sample	1603:1608	arg1	profile					1580:1586	almost same profile	1568:1586	almost same profile of large-scale sample	1568:1608	From 30 to 40 glycans were successfully detected with almost same profile of large-scale sample.
29945790	4	53	theme	constant	1019:1026	arg1	voltage					1028:1034	constant voltage	1019:1034	constant voltage	1019:1034	A typical LDIS-CE-LIF protocol was simple: a short-plug of leading electrolyte (LE) and large-volume sample solution were introduced to a capillary, followed by application of constant voltage.
29945790	3	54	theme	LDIS	722:725	arg1	"					727:727	stacking (LDIS)"	712:727	stacking (LDIS)"	712:727	For analyzing tiny biological samples, we developed sensitive, high-resolution, and salt-tolerant method for analyzing trace level of N-linked glycans by coupling capillary electrophoresis (CE), laser-induced fluorescence (LIF) detection, and a new online sample preconcentration (OSP) method named "large-volume dual preconcentration by isotachophoresis and stacking (LDIS)", which is composed of two OSP methods, large-volume sample stacking (LVSS) and transient isotachophoresis (tITP).
29945790	5	55	theme	sensitivity	1171:1181	arg1	increase					1183:1190	up to 2300-fold sensitivity increase	1155:1190	up to 2300-fold sensitivity increase	1155:1190	In the analysis of glucose ladder labeled with 8-aminopyrene-1,3,6-trisulfonic acid with 10 mM sodium chloride as LE, up to 2300-fold sensitivity increase was achieved with higher resolution than those in normal CE.
29945790	3	56	theme	stacking	712:719	arg1	"					727:727	stacking (LDIS)"	712:727	stacking (LDIS)"	712:727	For analyzing tiny biological samples, we developed sensitive, high-resolution, and salt-tolerant method for analyzing trace level of N-linked glycans by coupling capillary electrophoresis (CE), laser-induced fluorescence (LIF) detection, and a new online sample preconcentration (OSP) method named "large-volume dual preconcentration by isotachophoresis and stacking (LDIS)", which is composed of two OSP methods, large-volume sample stacking (LVSS) and transient isotachophoresis (tITP).
29945790	4	57	theme	LDIS-CE-LIF	853:863	arg1	simple					878:883	simple	878:883	simple	878:883	A typical LDIS-CE-LIF protocol was simple: a short-plug of leading electrolyte (LE) and large-volume sample solution were introduced to a capillary, followed by application of constant voltage.
29945790	4	57	theme	LDIS-CE-LIF	853:863	arg1	protocol					865:872	A typical LDIS-CE-LIF protocol	843:872	A typical LDIS-CE-LIF protocol	843:872	A typical LDIS-CE-LIF protocol was simple: a short-plug of leading electrolyte (LE) and large-volume sample solution were introduced to a capillary, followed by application of constant voltage.
29945790	8	58	theme	same	1575:1578	arg1	profile					1580:1586	almost same profile	1568:1586	almost same profile of large-scale sample	1568:1608	From 30 to 40 glycans were successfully detected with almost same profile of large-scale sample.
29945790	10	59	theme	N-linked	1910:1917	arg1	glycans					1919:1925	disease-specific N-linked glycans	1893:1925	disease-specific N-linked glycans	1893:1925	It suggests the potential of LDIS-CE-LIF for discovery of disease-specific N-linked glycans in microscale environment.
29945790	2	60	theme	early-stage	288:298	arg1	tissue					300:305	an early-stage tissue	285:305	an early-stage tissue	285:305	Profiling of glycans from limited number of pathogenetic cells in an early-stage tissue is essential for discovering effective drugs.
29945790	2	61	from	Profiling	219:227	arg1	tissue					300:305	an early-stage tissue	285:305	an early-stage tissue	285:305	Profiling of glycans from limited number of pathogenetic cells in an early-stage tissue is essential for discovering effective drugs.
29945790	2	61	from	Profiling	219:227	arg1	number					253:258	limited number	245:258	limited number of pathogenetic cells	245:280	Profiling of glycans from limited number of pathogenetic cells in an early-stage tissue is essential for discovering effective drugs.
29945790	2	62	theme	effective	336:344	arg1	drugs					346:350	effective drugs	336:350	effective drugs	336:350	Profiling of glycans from limited number of pathogenetic cells in an early-stage tissue is essential for discovering effective drugs.
29945790	7	63	from	cells	1431:1435	arg1	N-glycans					1398:1406	N-glycans	1398:1406	N-glycans from approximately 100 cells (HeLa, MCF7, and HepG2)	1398:1459	Finally, N-glycans from approximately 100 cells (HeLa, MCF7, and HepG2) were analyzed as the model of localized tumor cells.
29945790	7	63	from	cells	1431:1435	arg1	model					1482:1486	the model	1478:1486	the model of localized tumor cells	1478:1511	Finally, N-glycans from approximately 100 cells (HeLa, MCF7, and HepG2) were analyzed as the model of localized tumor cells.
29945790	6	64	theme	viscous	1311:1317	arg1	electrolyte					1323:1333	viscous gel electrolyte	1311:1333	viscous gel electrolyte	1311:1333	By applying pressure assist during preconcentration, both viscous gel electrolyte and salty matrix of up to 10 mM NaCl were acceptable.
29945790	3	65	theme	online	602:607	arg1	method					639:644	a new online sample preconcentration (OSP) method	596:644	a new online sample preconcentration (OSP) method named "large-volume dual preconcentration by isotachophoresis and stacking (LDIS)", which is composed of two OSP methods, large-volume sample stacking (LVSS) and transient isotachophoresis (tITP)	596:840	For analyzing tiny biological samples, we developed sensitive, high-resolution, and salt-tolerant method for analyzing trace level of N-linked glycans by coupling capillary electrophoresis (CE), laser-induced fluorescence (LIF) detection, and a new online sample preconcentration (OSP) method named "large-volume dual preconcentration by isotachophoresis and stacking (LDIS)", which is composed of two OSP methods, large-volume sample stacking (LVSS) and transient isotachophoresis (tITP).
29945790	4	66	theme	leading	902:908	arg1	LE					923:924	LE	923:924	LE	923:924	A typical LDIS-CE-LIF protocol was simple: a short-plug of leading electrolyte (LE) and large-volume sample solution were introduced to a capillary, followed by application of constant voltage.
29945790	4	66	theme	leading	902:908	arg1	electrolyte					910:920	leading electrolyte	902:920	leading electrolyte (LE)	902:925	A typical LDIS-CE-LIF protocol was simple: a short-plug of leading electrolyte (LE) and large-volume sample solution were introduced to a capillary, followed by application of constant voltage.
29945790	3	67	theme	large-volume	768:779	arg1	methods					759:765	two OSP methods	751:765	two OSP methods	751:765	For analyzing tiny biological samples, we developed sensitive, high-resolution, and salt-tolerant method for analyzing trace level of N-linked glycans by coupling capillary electrophoresis (CE), laser-induced fluorescence (LIF) detection, and a new online sample preconcentration (OSP) method named "large-volume dual preconcentration by isotachophoresis and stacking (LDIS)", which is composed of two OSP methods, large-volume sample stacking (LVSS) and transient isotachophoresis (tITP).
29945790	3	67	theme	large-volume	768:779	arg1	LVSS					798:801	LVSS	798:801	LVSS	798:801	For analyzing tiny biological samples, we developed sensitive, high-resolution, and salt-tolerant method for analyzing trace level of N-linked glycans by coupling capillary electrophoresis (CE), laser-induced fluorescence (LIF) detection, and a new online sample preconcentration (OSP) method named "large-volume dual preconcentration by isotachophoresis and stacking (LDIS)", which is composed of two OSP methods, large-volume sample stacking (LVSS) and transient isotachophoresis (tITP).
29945790	3	67	theme	large-volume	768:779	arg1	stacking					788:795	large-volume sample stacking	768:795	large-volume sample stacking (LVSS)	768:802	For analyzing tiny biological samples, we developed sensitive, high-resolution, and salt-tolerant method for analyzing trace level of N-linked glycans by coupling capillary electrophoresis (CE), laser-induced fluorescence (LIF) detection, and a new online sample preconcentration (OSP) method named "large-volume dual preconcentration by isotachophoresis and stacking (LDIS)", which is composed of two OSP methods, large-volume sample stacking (LVSS) and transient isotachophoresis (tITP).
29945790	3	67	theme	large-volume	768:779	arg1	tITP					836:839	tITP	836:839	tITP	836:839	For analyzing tiny biological samples, we developed sensitive, high-resolution, and salt-tolerant method for analyzing trace level of N-linked glycans by coupling capillary electrophoresis (CE), laser-induced fluorescence (LIF) detection, and a new online sample preconcentration (OSP) method named "large-volume dual preconcentration by isotachophoresis and stacking (LDIS)", which is composed of two OSP methods, large-volume sample stacking (LVSS) and transient isotachophoresis (tITP).
29945790	9	68	dep	HepG2	1721:1725	arg1	glycans					1753:1759	expressed more sialylated glycans	1727:1759	expressed more sialylated glycans	1727:1759	N-glycan structure could be predicted by searching glucose-unit value via Glycobase database, indicating that HepG2 expressed more sialylated glycans and MCF-7 expressed less glycans respectively, comparing with HeLa cells.
29945790	3	69	theme	OSP	634:636	arg1	method					639:644	a new online sample preconcentration (OSP) method	596:644	a new online sample preconcentration (OSP) method named "large-volume dual preconcentration by isotachophoresis and stacking (LDIS)", which is composed of two OSP methods, large-volume sample stacking (LVSS) and transient isotachophoresis (tITP)	596:840	For analyzing tiny biological samples, we developed sensitive, high-resolution, and salt-tolerant method for analyzing trace level of N-linked glycans by coupling capillary electrophoresis (CE), laser-induced fluorescence (LIF) detection, and a new online sample preconcentration (OSP) method named "large-volume dual preconcentration by isotachophoresis and stacking (LDIS)", which is composed of two OSP methods, large-volume sample stacking (LVSS) and transient isotachophoresis (tITP).
29945790	6	70	theme	NaCl	1367:1370	arg1	matrix					1345:1350	salty matrix	1339:1350	salty matrix of up to 10 mM NaCl	1339:1370	By applying pressure assist during preconcentration, both viscous gel electrolyte and salty matrix of up to 10 mM NaCl were acceptable.
29945790	6	70	theme	NaCl	1367:1370	arg1	electrolyte					1323:1333	viscous gel electrolyte	1311:1333	viscous gel electrolyte	1311:1333	By applying pressure assist during preconcentration, both viscous gel electrolyte and salty matrix of up to 10 mM NaCl were acceptable.
29945790	3	71	theme	preconcentration	616:631	arg1	method					639:644	a new online sample preconcentration (OSP) method	596:644	a new online sample preconcentration (OSP) method named "large-volume dual preconcentration by isotachophoresis and stacking (LDIS)", which is composed of two OSP methods, large-volume sample stacking (LVSS) and transient isotachophoresis (tITP)	596:840	For analyzing tiny biological samples, we developed sensitive, high-resolution, and salt-tolerant method for analyzing trace level of N-linked glycans by coupling capillary electrophoresis (CE), laser-induced fluorescence (LIF) detection, and a new online sample preconcentration (OSP) method named "large-volume dual preconcentration by isotachophoresis and stacking (LDIS)", which is composed of two OSP methods, large-volume sample stacking (LVSS) and transient isotachophoresis (tITP).
29945790	4	72	theme	large-volume	931:942	arg1	solution					951:958	large-volume sample solution	931:958	large-volume sample solution	931:958	A typical LDIS-CE-LIF protocol was simple: a short-plug of leading electrolyte (LE) and large-volume sample solution were introduced to a capillary, followed by application of constant voltage.
29945790	0	73	theme	large-volume	79:90	arg1	preconcentration					97:112	large-volume dual preconcentration	79:112	large-volume dual preconcentration by isotachophoresis and stacking	79:145	Profiling of N-linked glycans from 100 cells by capillary electrophoresis with large-volume dual preconcentration by isotachophoresis and stacking.
29945790	9	74	gly	sialylated	1742:1751	arg1	glycans					1753:1759	expressed more sialylated glycans	1727:1759	expressed more sialylated glycans	1727:1759	N-glycan structure could be predicted by searching glucose-unit value via Glycobase database, indicating that HepG2 expressed more sialylated glycans and MCF-7 expressed less glycans respectively, comparing with HeLa cells.
29945790	7	75	theme	cells	1507:1511	arg1	N-glycans					1398:1406	N-glycans	1398:1406	N-glycans from approximately 100 cells (HeLa, MCF7, and HepG2)	1398:1459	Finally, N-glycans from approximately 100 cells (HeLa, MCF7, and HepG2) were analyzed as the model of localized tumor cells.
29945790	7	75	theme	cells	1507:1511	arg1	model					1482:1486	the model	1478:1486	the model of localized tumor cells	1478:1511	Finally, N-glycans from approximately 100 cells (HeLa, MCF7, and HepG2) were analyzed as the model of localized tumor cells.
29945790	4	76	theme	solution	951:958	arg1	short-plug					888:897	a short-plug	886:897	a short-plug of leading electrolyte (LE) and large-volume sample solution	886:958	A typical LDIS-CE-LIF protocol was simple: a short-plug of leading electrolyte (LE) and large-volume sample solution were introduced to a capillary, followed by application of constant voltage.
29945790	5	77	theme	normal	1242:1247	arg1	CE					1249:1250	normal CE	1242:1250	normal CE	1242:1250	In the analysis of glucose ladder labeled with 8-aminopyrene-1,3,6-trisulfonic acid with 10 mM sodium chloride as LE, up to 2300-fold sensitivity increase was achieved with higher resolution than those in normal CE.
29945790	5	78	theme	10 mM	1126:1130	arg1	chloride					1139:1146	10 mM sodium chloride	1126:1146	10 mM sodium chloride	1126:1146	In the analysis of glucose ladder labeled with 8-aminopyrene-1,3,6-trisulfonic acid with 10 mM sodium chloride as LE, up to 2300-fold sensitivity increase was achieved with higher resolution than those in normal CE.
29945790	7	79	theme	localized	1491:1499	arg1	cells					1507:1511	localized tumor cells	1491:1511	localized tumor cells	1491:1511	Finally, N-glycans from approximately 100 cells (HeLa, MCF7, and HepG2) were analyzed as the model of localized tumor cells.
29945790	6	80	theme	salty	1339:1343	arg1	matrix					1345:1350	salty matrix	1339:1350	salty matrix of up to 10 mM NaCl	1339:1370	By applying pressure assist during preconcentration, both viscous gel electrolyte and salty matrix of up to 10 mM NaCl were acceptable.
29945790	5	81	theme	ladder	1064:1069	arg1	analysis					1044:1051	the analysis	1040:1051	the analysis of glucose ladder labeled with 8-aminopyrene-1,3,6-trisulfonic acid with 10 mM sodium chloride as LE	1040:1152	In the analysis of glucose ladder labeled with 8-aminopyrene-1,3,6-trisulfonic acid with 10 mM sodium chloride as LE, up to 2300-fold sensitivity increase was achieved with higher resolution than those in normal CE.
29945790	3	82	theme	biological	372:381	arg1	samples					383:389	tiny biological samples	367:389	tiny biological samples	367:389	For analyzing tiny biological samples, we developed sensitive, high-resolution, and salt-tolerant method for analyzing trace level of N-linked glycans by coupling capillary electrophoresis (CE), laser-induced fluorescence (LIF) detection, and a new online sample preconcentration (OSP) method named "large-volume dual preconcentration by isotachophoresis and stacking (LDIS)", which is composed of two OSP methods, large-volume sample stacking (LVSS) and transient isotachophoresis (tITP).
29945790	3	83	theme	trace	472:476	arg1	level					478:482	trace level	472:482	trace level of N-linked glycans	472:502	For analyzing tiny biological samples, we developed sensitive, high-resolution, and salt-tolerant method for analyzing trace level of N-linked glycans by coupling capillary electrophoresis (CE), laser-induced fluorescence (LIF) detection, and a new online sample preconcentration (OSP) method named "large-volume dual preconcentration by isotachophoresis and stacking (LDIS)", which is composed of two OSP methods, large-volume sample stacking (LVSS) and transient isotachophoresis (tITP).
29945790	0	84	theme	glycans	22:28	arg1	Profiling					0:8	Profiling	0:8	Profiling of N-linked glycans from 100 cells by capillary electrophoresis with large-volume dual preconcentration by isotachophoresis and stacking.	0:146	Profiling of N-linked glycans from 100 cells by capillary electrophoresis with large-volume dual preconcentration by isotachophoresis and stacking.
29945790	9	85	theme	less	1781:1784	arg1	glycans					1786:1792	less glycans	1781:1792	less glycans	1781:1792	N-glycan structure could be predicted by searching glucose-unit value via Glycobase database, indicating that HepG2 expressed more sialylated glycans and MCF-7 expressed less glycans respectively, comparing with HeLa cells.
29945790	3	86	theme	glycans	496:502	arg1	level					478:482	trace level	472:482	trace level of N-linked glycans	472:502	For analyzing tiny biological samples, we developed sensitive, high-resolution, and salt-tolerant method for analyzing trace level of N-linked glycans by coupling capillary electrophoresis (CE), laser-induced fluorescence (LIF) detection, and a new online sample preconcentration (OSP) method named "large-volume dual preconcentration by isotachophoresis and stacking (LDIS)", which is composed of two OSP methods, large-volume sample stacking (LVSS) and transient isotachophoresis (tITP).
29945790	5	87	theme	2300-fold	1161:1169	arg1	increase					1183:1190	up to 2300-fold sensitivity increase	1155:1190	up to 2300-fold sensitivity increase	1155:1190	In the analysis of glucose ladder labeled with 8-aminopyrene-1,3,6-trisulfonic acid with 10 mM sodium chloride as LE, up to 2300-fold sensitivity increase was achieved with higher resolution than those in normal CE.
29945790	3	88	theme	salt-tolerant	437:449	arg1	method					451:456	sensitive, high-resolution, and salt-tolerant method	405:456	sensitive, high-resolution, and salt-tolerant method for analyzing trace level of N-linked glycans by coupling capillary electrophoresis (CE), laser-induced fluorescence (LIF) detection, and a new online sample preconcentration (OSP) method named "large-volume dual preconcentration by isotachophoresis and stacking (LDIS)", which is composed of two OSP methods, large-volume sample stacking (LVSS) and transient isotachophoresis (tITP)	405:840	For analyzing tiny biological samples, we developed sensitive, high-resolution, and salt-tolerant method for analyzing trace level of N-linked glycans by coupling capillary electrophoresis (CE), laser-induced fluorescence (LIF) detection, and a new online sample preconcentration (OSP) method named "large-volume dual preconcentration by isotachophoresis and stacking (LDIS)", which is composed of two OSP methods, large-volume sample stacking (LVSS) and transient isotachophoresis (tITP).
29945790	7	89	dep	cells	1431:1435	arg1	MCF7					1444:1447	MCF7	1444:1447	MCF7	1444:1447	Finally, N-glycans from approximately 100 cells (HeLa, MCF7, and HepG2) were analyzed as the model of localized tumor cells.
29945790	7	89	dep	cells	1431:1435	arg1	HepG2					1454:1458	HepG2	1454:1458	HepG2	1454:1458	Finally, N-glycans from approximately 100 cells (HeLa, MCF7, and HepG2) were analyzed as the model of localized tumor cells.
29945790	7	89	dep	cells	1431:1435	arg1	HeLa					1438:1441	HeLa	1438:1441	HeLa	1438:1441	Finally, N-glycans from approximately 100 cells (HeLa, MCF7, and HepG2) were analyzed as the model of localized tumor cells.
29945790	7	89	dep	cells	1431:1435	arg1	cells					1431:1435	approximately 100 cells	1413:1435	approximately 100 cells (HeLa, MCF7, and HepG2)	1413:1459	Finally, N-glycans from approximately 100 cells (HeLa, MCF7, and HepG2) were analyzed as the model of localized tumor cells.
29945790	9	90	theme	glucose-unit	1662:1673	arg1	value					1675:1679	glucose-unit value	1662:1679	glucose-unit value	1662:1679	N-glycan structure could be predicted by searching glucose-unit value via Glycobase database, indicating that HepG2 expressed more sialylated glycans and MCF-7 expressed less glycans respectively, comparing with HeLa cells.
29945790	8	91	theme	large-scale	1591:1601	arg1	sample					1603:1608	large-scale sample	1591:1608	large-scale sample	1591:1608	From 30 to 40 glycans were successfully detected with almost same profile of large-scale sample.
29945790	3	92	theme	sample	609:614	arg1	method					639:644	a new online sample preconcentration (OSP) method	596:644	a new online sample preconcentration (OSP) method named "large-volume dual preconcentration by isotachophoresis and stacking (LDIS)", which is composed of two OSP methods, large-volume sample stacking (LVSS) and transient isotachophoresis (tITP)	596:840	For analyzing tiny biological samples, we developed sensitive, high-resolution, and salt-tolerant method for analyzing trace level of N-linked glycans by coupling capillary electrophoresis (CE), laser-induced fluorescence (LIF) detection, and a new online sample preconcentration (OSP) method named "large-volume dual preconcentration by isotachophoresis and stacking (LDIS)", which is composed of two OSP methods, large-volume sample stacking (LVSS) and transient isotachophoresis (tITP).
29945790	9	93	theme	HeLa	1823:1826	arg1	cells					1828:1832	HeLa cells	1823:1832	HeLa cells	1823:1832	N-glycan structure could be predicted by searching glucose-unit value via Glycobase database, indicating that HepG2 expressed more sialylated glycans and MCF-7 expressed less glycans respectively, comparing with HeLa cells.
29945790	3	94	theme	dual	666:669	arg1	preconcentration					671:686	"large-volume dual preconcentration	652:686	"large-volume dual preconcentration	652:686	For analyzing tiny biological samples, we developed sensitive, high-resolution, and salt-tolerant method for analyzing trace level of N-linked glycans by coupling capillary electrophoresis (CE), laser-induced fluorescence (LIF) detection, and a new online sample preconcentration (OSP) method named "large-volume dual preconcentration by isotachophoresis and stacking (LDIS)", which is composed of two OSP methods, large-volume sample stacking (LVSS) and transient isotachophoresis (tITP).
29945790	3	95	theme	coupling	507:514	arg1	CE					543:544	CE	543:544	CE	543:544	For analyzing tiny biological samples, we developed sensitive, high-resolution, and salt-tolerant method for analyzing trace level of N-linked glycans by coupling capillary electrophoresis (CE), laser-induced fluorescence (LIF) detection, and a new online sample preconcentration (OSP) method named "large-volume dual preconcentration by isotachophoresis and stacking (LDIS)", which is composed of two OSP methods, large-volume sample stacking (LVSS) and transient isotachophoresis (tITP).
29945790	3	95	theme	coupling	507:514	arg1	electrophoresis					526:540	coupling capillary electrophoresis	507:540	coupling capillary electrophoresis (CE)	507:545	For analyzing tiny biological samples, we developed sensitive, high-resolution, and salt-tolerant method for analyzing trace level of N-linked glycans by coupling capillary electrophoresis (CE), laser-induced fluorescence (LIF) detection, and a new online sample preconcentration (OSP) method named "large-volume dual preconcentration by isotachophoresis and stacking (LDIS)", which is composed of two OSP methods, large-volume sample stacking (LVSS) and transient isotachophoresis (tITP).
29945790	5	96	theme	higher	1210:1215	arg1	resolution					1217:1226	higher resolution	1210:1226	higher resolution than those in normal CE	1210:1250	In the analysis of glucose ladder labeled with 8-aminopyrene-1,3,6-trisulfonic acid with 10 mM sodium chloride as LE, up to 2300-fold sensitivity increase was achieved with higher resolution than those in normal CE.
31685900	8	0	theme	prediction	1236:1245	arg1	score					1247:1251	the first-stage prediction score	1220:1251	the first-stage prediction score	1220:1251	N-GlyDE's final predictions are derived from a weight adjustment of the second-stage prediction results based on the first-stage prediction score.
31685900	4	1	from	asparagine	469:478	arg1	performance					448:458	their performance	442:458	their performance at every asparagine in protein sequences	442:499	Most of them evaluate their performance at every asparagine in protein sequences, not confined to asparagine in the N-X-S/T sequon.
31685900	1	2	theme	post-translational	169:186	arg1	modifications					188:200	the predominant post-translational modifications	153:200	the predominant post-translational modifications involved in a number of biological functions	153:245	N-linked glycosylation is one of the predominant post-translational modifications involved in a number of biological functions.
31685900	6	3	gly	non-glycoproteins	819:835	arg1	non-glycoproteins					819:835	non-glycoproteins	819:835	non-glycoproteins	819:835	The first stage uses a protein similarity voting algorithm trained on both glycoproteins and non-glycoproteins to predict a score for a protein to improve glycosite prediction.
31685900	7	4	theme	second	907:912	arg1	stage					914:918	The second stage	903:918	The second stage	903:918	The second stage uses a support vector machine to predict N-linked glycosites by utilizing features of gapped dipeptides, pattern-based predicted surface accessibility, and predicted secondary structure.
31685900	9	5	gly	non-glycoproteins	1346:1362	arg1	non-glycoproteins					1346:1362	33 non-glycoproteins	1343:1362	33 non-glycoproteins	1343:1362	Evaluated on N-X-S/T sequons of an independent dataset comprised of 53 glycoproteins and 33 non-glycoproteins, N-GlyDE achieves an accuracy and MCC of 0.740 and 0.499, respectively, outperforming the compared tools.
31685900	5	6	theme	human	710:714	arg1	proteome					716:723	the human proteome	706:723	the human proteome	706:723	In this paper, we present N-GlyDE, a two-stage prediction tool trained on rigorously-constructed non-redundant datasets to predict N-linked glycosites in the human proteome.
31685900	5	7	theme	rigorously-constructed	626:647	arg1	datasets					663:670	rigorously-constructed non-redundant datasets	626:670	rigorously-constructed non-redundant datasets	626:670	In this paper, we present N-GlyDE, a two-stage prediction tool trained on rigorously-constructed non-redundant datasets to predict N-linked glycosites in the human proteome.
31685900	1	8	theme	modifications	188:200	arg1	one					146:148	one	146:148	one	146:148	N-linked glycosylation is one of the predominant post-translational modifications involved in a number of biological functions.
31685900	1	8	theme	modifications	188:200	arg1	modifications					188:200	the predominant post-translational modifications	153:200	the predominant post-translational modifications involved in a number of biological functions	153:245	N-linked glycosylation is one of the predominant post-translational modifications involved in a number of biological functions.
31685900	7	9	theme	support	927:933	arg1	machine					942:948	a support vector machine	925:948	a support vector machine	925:948	The second stage uses a support vector machine to predict N-linked glycosites by utilizing features of gapped dipeptides, pattern-based predicted surface accessibility, and predicted secondary structure.
31685900	6	10	theme	glycosite	881:889	arg1	prediction					891:900	glycosite prediction	881:900	glycosite prediction	881:900	The first stage uses a protein similarity voting algorithm trained on both glycoproteins and non-glycoproteins to predict a score for a protein to improve glycosite prediction.
31685900	7	11	theme	predicted	1039:1047	arg1	accessibility					1057:1069	pattern-based predicted surface accessibility	1025:1069	pattern-based predicted surface accessibility	1025:1069	The second stage uses a support vector machine to predict N-linked glycosites by utilizing features of gapped dipeptides, pattern-based predicted surface accessibility, and predicted secondary structure.
31685900	5	12	theme	non-redundant	649:661	arg1	datasets					663:670	rigorously-constructed non-redundant datasets	626:670	rigorously-constructed non-redundant datasets	626:670	In this paper, we present N-GlyDE, a two-stage prediction tool trained on rigorously-constructed non-redundant datasets to predict N-linked glycosites in the human proteome.
31685900	7	13	theme	vector	935:940	arg1	machine					942:948	a support vector machine	925:948	a support vector machine	925:948	The second stage uses a support vector machine to predict N-linked glycosites by utilizing features of gapped dipeptides, pattern-based predicted surface accessibility, and predicted secondary structure.
31685900	6	14	gly	glycoproteins	801:813	arg1	glycoproteins					801:813	glycoproteins	801:813	glycoproteins	801:813	The first stage uses a protein similarity voting algorithm trained on both glycoproteins and non-glycoproteins to predict a score for a protein to improve glycosite prediction.
31685900	0	15	theme	pattern-based	96:108	arg1	encoding					110:117	pattern-based encoding	96:117	pattern-based encoding	96:117	N-GlyDE: a two-stage N-linked glycosylation site prediction incorporating gapped dipeptides and pattern-based encoding.
31685900	8	16	theme	final	1117:1121	arg1	predictions					1123:1133	N-GlyDE's final predictions	1107:1133	N-GlyDE's final predictions	1107:1133	N-GlyDE's final predictions are derived from a weight adjustment of the second-stage prediction results based on the first-stage prediction score.
31685900	7	17	theme	dipeptides	1013:1022	arg1	features					994:1001	features	994:1001	features of gapped dipeptides, pattern-based predicted surface accessibility, and predicted secondary structure	994:1104	The second stage uses a support vector machine to predict N-linked glycosites by utilizing features of gapped dipeptides, pattern-based predicted surface accessibility, and predicted secondary structure.
31685900	10	18	from	http	1509:1512	arg1	available					1496:1504	available	1496:1504	available	1496:1504	The N-GlyDE web server is available at http://bioapp.iis.sinica.edu.tw/N-GlyDE/ .
31685900	10	18	from	http	1509:1512	arg1	server					1486:1491	The N-GlyDE web server	1470:1491	The N-GlyDE web server	1470:1491	The N-GlyDE web server is available at http://bioapp.iis.sinica.edu.tw/N-GlyDE/ .
31685900	2	19	gly	glycosite	314:322	arg2	glycosite					314:322	glycosite	314:322	glycosite	314:322	Since experimental characterization of glycosites is challenging, glycosite prediction is crucial.
31685900	9	20	theme	0.740	1405:1409	arg1	MCC					1398:1400	MCC	1398:1400	MCC	1398:1400	Evaluated on N-X-S/T sequons of an independent dataset comprised of 53 glycoproteins and 33 non-glycoproteins, N-GlyDE achieves an accuracy and MCC of 0.740 and 0.499, respectively, outperforming the compared tools.
31685900	9	20	theme	0.740	1405:1409	arg1	accuracy					1385:1392	accuracy	1385:1392	accuracy	1385:1392	Evaluated on N-X-S/T sequons of an independent dataset comprised of 53 glycoproteins and 33 non-glycoproteins, N-GlyDE achieves an accuracy and MCC of 0.740 and 0.499, respectively, outperforming the compared tools.
31685900	8	21	attach	derived	1139:1145	arg2	predictions					1123:1133	N-GlyDE's final predictions	1107:1133	N-GlyDE's final predictions	1107:1133	N-GlyDE's final predictions are derived from a weight adjustment of the second-stage prediction results based on the first-stage prediction score.
31685900	8	21	attach	derived	1139:1145	arg1	adjustment					1161:1170	a weight adjustment	1152:1170	a weight adjustment of the second-stage prediction results based on the first-stage prediction score	1152:1251	N-GlyDE's final predictions are derived from a weight adjustment of the second-stage prediction results based on the first-stage prediction score.
31685900	4	22	from	performance	448:458	arg1	sequences					491:499	protein sequences	483:499	protein sequences	483:499	Most of them evaluate their performance at every asparagine in protein sequences, not confined to asparagine in the N-X-S/T sequon.
31685900	9	23	gly	glycoproteins	1325:1337	arg1	glycoproteins					1325:1337	53 glycoproteins	1322:1337	53 glycoproteins	1322:1337	Evaluated on N-X-S/T sequons of an independent dataset comprised of 53 glycoproteins and 33 non-glycoproteins, N-GlyDE achieves an accuracy and MCC of 0.740 and 0.499, respectively, outperforming the compared tools.
31685900	3	24	theme	high	402:405	arg1	performance					407:417	high performance	402:417	high performance	402:417	Several predictors have been made available and report high performance.
31685900	4	25	theme	N-X-S/T	536:542	arg1	sequon					544:549	the N-X-S/T sequon	532:549	the N-X-S/T sequon	532:549	Most of them evaluate their performance at every asparagine in protein sequences, not confined to asparagine in the N-X-S/T sequon.
31685900	5	26	theme	N-linked	683:690	arg1	glycosites					692:701	N-linked glycosites	683:701	N-linked glycosites in the human proteome	683:723	In this paper, we present N-GlyDE, a two-stage prediction tool trained on rigorously-constructed non-redundant datasets to predict N-linked glycosites in the human proteome.
31685900	5	27	theme	prediction	599:608	arg1	tool					610:613	a two-stage prediction tool	587:613	a two-stage prediction tool trained on rigorously-constructed non-redundant datasets	587:670	In this paper, we present N-GlyDE, a two-stage prediction tool trained on rigorously-constructed non-redundant datasets to predict N-linked glycosites in the human proteome.
31685900	5	27	theme	prediction	599:608	arg1	N-GlyDE					578:584	N-GlyDE	578:584	N-GlyDE	578:584	In this paper, we present N-GlyDE, a two-stage prediction tool trained on rigorously-constructed non-redundant datasets to predict N-linked glycosites in the human proteome.
31685900	2	28	theme	glycosite	314:322	arg1	prediction					324:333	glycosite prediction	314:333	glycosite prediction	314:333	Since experimental characterization of glycosites is challenging, glycosite prediction is crucial.
31685900	6	29	gly	glycosite	881:889	arg2	glycosite					881:889	glycosite prediction	881:900	glycosite prediction	881:900	The first stage uses a protein similarity voting algorithm trained on both glycoproteins and non-glycoproteins to predict a score for a protein to improve glycosite prediction.
31685900	5	30	attach	present	570:576	arg1	paper					560:564	this paper	555:564	this paper	555:564	In this paper, we present N-GlyDE, a two-stage prediction tool trained on rigorously-constructed non-redundant datasets to predict N-linked glycosites in the human proteome.
31685900	5	30	attach	present	570:576	arg2	we					567:568	we	567:568	we	567:568	In this paper, we present N-GlyDE, a two-stage prediction tool trained on rigorously-constructed non-redundant datasets to predict N-linked glycosites in the human proteome.
31685900	8	31	theme	first-stage	1224:1234	arg1	score					1247:1251	the first-stage prediction score	1220:1251	the first-stage prediction score	1220:1251	N-GlyDE's final predictions are derived from a weight adjustment of the second-stage prediction results based on the first-stage prediction score.
31685900	7	32	theme	gapped	1006:1011	arg1	dipeptides					1013:1022	gapped dipeptides	1006:1022	gapped dipeptides	1006:1022	The second stage uses a support vector machine to predict N-linked glycosites by utilizing features of gapped dipeptides, pattern-based predicted surface accessibility, and predicted secondary structure.
31685900	9	33	theme	0.499	1415:1419	arg1	MCC					1398:1400	MCC	1398:1400	MCC	1398:1400	Evaluated on N-X-S/T sequons of an independent dataset comprised of 53 glycoproteins and 33 non-glycoproteins, N-GlyDE achieves an accuracy and MCC of 0.740 and 0.499, respectively, outperforming the compared tools.
31685900	9	33	theme	0.499	1415:1419	arg1	accuracy					1385:1392	accuracy	1385:1392	accuracy	1385:1392	Evaluated on N-X-S/T sequons of an independent dataset comprised of 53 glycoproteins and 33 non-glycoproteins, N-GlyDE achieves an accuracy and MCC of 0.740 and 0.499, respectively, outperforming the compared tools.
31685900	5	34	from	glycosites	692:701	arg1	proteome					716:723	the human proteome	706:723	the human proteome	706:723	In this paper, we present N-GlyDE, a two-stage prediction tool trained on rigorously-constructed non-redundant datasets to predict N-linked glycosites in the human proteome.
31685900	4	35	from	asparagine	518:527	arg1	sequon					544:549	the N-X-S/T sequon	532:549	the N-X-S/T sequon	532:549	Most of them evaluate their performance at every asparagine in protein sequences, not confined to asparagine in the N-X-S/T sequon.
31685900	5	36	link	N-linked	683:690	arg1	glycosites					692:701	N-linked glycosites	683:701	N-linked glycosites in the human proteome	683:723	In this paper, we present N-GlyDE, a two-stage prediction tool trained on rigorously-constructed non-redundant datasets to predict N-linked glycosites in the human proteome.
31685900	2	37	gly	glycosites	287:296	arg2	glycosites					287:296	glycosites	287:296	glycosites	287:296	Since experimental characterization of glycosites is challenging, glycosite prediction is crucial.
31685900	0	38	link	N-linked	21:28	arg1	prediction					49:58	a two-stage N-linked glycosylation site prediction	9:58	N-GlyDE: a two-stage N-linked glycosylation site prediction incorporating gapped dipeptides and pattern-based encoding.	0:118	N-GlyDE: a two-stage N-linked glycosylation site prediction incorporating gapped dipeptides and pattern-based encoding.
31685900	7	39	theme	pattern-based	1025:1037	arg1	accessibility					1057:1069	pattern-based predicted surface accessibility	1025:1069	pattern-based predicted surface accessibility	1025:1069	The second stage uses a support vector machine to predict N-linked glycosites by utilizing features of gapped dipeptides, pattern-based predicted surface accessibility, and predicted secondary structure.
31685900	8	40	theme	weight	1154:1159	arg1	adjustment					1161:1170	a weight adjustment	1152:1170	a weight adjustment of the second-stage prediction results based on the first-stage prediction score	1152:1251	N-GlyDE's final predictions are derived from a weight adjustment of the second-stage prediction results based on the first-stage prediction score.
31685900	7	41	theme	secondary	1086:1094	arg1	structure					1096:1104	predicted secondary structure	1076:1104	predicted secondary structure	1076:1104	The second stage uses a support vector machine to predict N-linked glycosites by utilizing features of gapped dipeptides, pattern-based predicted surface accessibility, and predicted secondary structure.
31685900	1	42	theme	N-linked	120:127	arg1	glycosylation					129:141	N-linked glycosylation	120:141	N-linked glycosylation	120:141	N-linked glycosylation is one of the predominant post-translational modifications involved in a number of biological functions.
31685900	6	43	theme	first	730:734	arg1	stage					736:740	The first stage	726:740	The first stage	726:740	The first stage uses a protein similarity voting algorithm trained on both glycoproteins and non-glycoproteins to predict a score for a protein to improve glycosite prediction.
31685900	1	44	theme	biological	226:235	arg1	functions					237:245	biological functions	226:245	biological functions	226:245	N-linked glycosylation is one of the predominant post-translational modifications involved in a number of biological functions.
31685900	0	45	theme	glycosylation	30:42	arg1	prediction					49:58	a two-stage N-linked glycosylation site prediction	9:58	N-GlyDE: a two-stage N-linked glycosylation site prediction incorporating gapped dipeptides and pattern-based encoding.	0:118	N-GlyDE: a two-stage N-linked glycosylation site prediction incorporating gapped dipeptides and pattern-based encoding.
31685900	9	46	dep	accuracy	1385:1392	arg1	an					1382:1383	an	1382:1383	an	1382:1383	Evaluated on N-X-S/T sequons of an independent dataset comprised of 53 glycoproteins and 33 non-glycoproteins, N-GlyDE achieves an accuracy and MCC of 0.740 and 0.499, respectively, outperforming the compared tools.
31685900	1	47	theme	functions	237:245	arg1	number					216:221	a number	214:221	a number of biological functions	214:245	N-linked glycosylation is one of the predominant post-translational modifications involved in a number of biological functions.
31685900	0	48	theme	N-linked	21:28	arg1	prediction					49:58	a two-stage N-linked glycosylation site prediction	9:58	N-GlyDE: a two-stage N-linked glycosylation site prediction incorporating gapped dipeptides and pattern-based encoding.	0:118	N-GlyDE: a two-stage N-linked glycosylation site prediction incorporating gapped dipeptides and pattern-based encoding.
31685900	0	49	theme	two-stage	11:19	arg1	prediction					49:58	a two-stage N-linked glycosylation site prediction	9:58	N-GlyDE: a two-stage N-linked glycosylation site prediction incorporating gapped dipeptides and pattern-based encoding.	0:118	N-GlyDE: a two-stage N-linked glycosylation site prediction incorporating gapped dipeptides and pattern-based encoding.
31685900	7	50	theme	structure	1096:1104	arg1	features					994:1001	features	994:1001	features of gapped dipeptides, pattern-based predicted surface accessibility, and predicted secondary structure	994:1104	The second stage uses a support vector machine to predict N-linked glycosites by utilizing features of gapped dipeptides, pattern-based predicted surface accessibility, and predicted secondary structure.
31685900	5	51	gly	glycosites	692:701	arg2	glycosites					692:701	N-linked glycosites	683:701	N-linked glycosites in the human proteome	683:723	In this paper, we present N-GlyDE, a two-stage prediction tool trained on rigorously-constructed non-redundant datasets to predict N-linked glycosites in the human proteome.
31685900	9	52	theme	compared	1454:1461	arg1	tools					1463:1467	the compared tools	1450:1467	the compared tools	1450:1467	Evaluated on N-X-S/T sequons of an independent dataset comprised of 53 glycoproteins and 33 non-glycoproteins, N-GlyDE achieves an accuracy and MCC of 0.740 and 0.499, respectively, outperforming the compared tools.
31685900	8	53	theme	prediction	1192:1201	arg1	results					1203:1209	the second-stage prediction results	1175:1209	the second-stage prediction results based on the first-stage prediction score	1175:1251	N-GlyDE's final predictions are derived from a weight adjustment of the second-stage prediction results based on the first-stage prediction score.
31685900	10	54	theme	web	1482:1484	arg1	available					1496:1504	available	1496:1504	available	1496:1504	The N-GlyDE web server is available at http://bioapp.iis.sinica.edu.tw/N-GlyDE/ .
31685900	10	54	theme	web	1482:1484	arg1	server					1486:1491	The N-GlyDE web server	1470:1491	The N-GlyDE web server	1470:1491	The N-GlyDE web server is available at http://bioapp.iis.sinica.edu.tw/N-GlyDE/ .
31685900	2	55	theme	experimental	254:265	arg1	characterization					267:282	experimental characterization	254:282	experimental characterization of glycosites	254:296	Since experimental characterization of glycosites is challenging, glycosite prediction is crucial.
31685900	9	56	theme	N-X-S/T	1267:1273	arg1	sequons					1275:1281	N-X-S/T sequons	1267:1281	N-X-S/T sequons of an independent dataset comprised of 53 glycoproteins and 33 non-glycoproteins	1267:1362	Evaluated on N-X-S/T sequons of an independent dataset comprised of 53 glycoproteins and 33 non-glycoproteins, N-GlyDE achieves an accuracy and MCC of 0.740 and 0.499, respectively, outperforming the compared tools.
31685900	0	57	theme	site	44:47	arg1	prediction					49:58	a two-stage N-linked glycosylation site prediction	9:58	N-GlyDE: a two-stage N-linked glycosylation site prediction incorporating gapped dipeptides and pattern-based encoding.	0:118	N-GlyDE: a two-stage N-linked glycosylation site prediction incorporating gapped dipeptides and pattern-based encoding.
31685900	7	58	theme	accessibility	1057:1069	arg1	features					994:1001	features	994:1001	features of gapped dipeptides, pattern-based predicted surface accessibility, and predicted secondary structure	994:1104	The second stage uses a support vector machine to predict N-linked glycosites by utilizing features of gapped dipeptides, pattern-based predicted surface accessibility, and predicted secondary structure.
31685900	8	59	theme	second-stage	1179:1190	arg1	results					1203:1209	the second-stage prediction results	1175:1209	the second-stage prediction results based on the first-stage prediction score	1175:1251	N-GlyDE's final predictions are derived from a weight adjustment of the second-stage prediction results based on the first-stage prediction score.
31685900	7	60	gly	glycosites	970:979	arg2	glycosites					970:979	N-linked glycosites	961:979	N-linked glycosites	961:979	The second stage uses a support vector machine to predict N-linked glycosites by utilizing features of gapped dipeptides, pattern-based predicted surface accessibility, and predicted secondary structure.
31685900	7	61	theme	predicted	1076:1084	arg1	structure					1096:1104	predicted secondary structure	1076:1104	predicted secondary structure	1076:1104	The second stage uses a support vector machine to predict N-linked glycosites by utilizing features of gapped dipeptides, pattern-based predicted surface accessibility, and predicted secondary structure.
31685900	4	62	dep	evaluate	433:440	arg1	confined					506:513	confined	506:513	not confined to asparagine in the N-X-S/T sequon	502:549	Most of them evaluate their performance at every asparagine in protein sequences, not confined to asparagine in the N-X-S/T sequon.
31685900	0	63	dep	N-GlyDE	0:6	arg1	prediction					49:58	a two-stage N-linked glycosylation site prediction	9:58	N-GlyDE: a two-stage N-linked glycosylation site prediction incorporating gapped dipeptides and pattern-based encoding.	0:118	N-GlyDE: a two-stage N-linked glycosylation site prediction incorporating gapped dipeptides and pattern-based encoding.
31685900	7	64	link	N-linked	961:968	arg1	glycosites					970:979	N-linked glycosites	961:979	N-linked glycosites	961:979	The second stage uses a support vector machine to predict N-linked glycosites by utilizing features of gapped dipeptides, pattern-based predicted surface accessibility, and predicted secondary structure.
31685900	5	65	theme	two-stage	589:597	arg1	tool					610:613	a two-stage prediction tool	587:613	a two-stage prediction tool trained on rigorously-constructed non-redundant datasets	587:670	In this paper, we present N-GlyDE, a two-stage prediction tool trained on rigorously-constructed non-redundant datasets to predict N-linked glycosites in the human proteome.
31685900	5	65	theme	two-stage	589:597	arg1	N-GlyDE					578:584	N-GlyDE	578:584	N-GlyDE	578:584	In this paper, we present N-GlyDE, a two-stage prediction tool trained on rigorously-constructed non-redundant datasets to predict N-linked glycosites in the human proteome.
31685900	9	66	theme	independent	1289:1299	arg1	dataset					1301:1307	an independent dataset	1286:1307	an independent dataset comprised of 53 glycoproteins and 33 non-glycoproteins	1286:1362	Evaluated on N-X-S/T sequons of an independent dataset comprised of 53 glycoproteins and 33 non-glycoproteins, N-GlyDE achieves an accuracy and MCC of 0.740 and 0.499, respectively, outperforming the compared tools.
31685900	3	67	theme	Several	347:353	arg1	predictors					355:364	Several predictors	347:364	Several predictors	347:364	Several predictors have been made available and report high performance.
31685900	0	68	gly	glycosylation	30:42	arg2	prediction					49:58	a two-stage N-linked glycosylation site prediction	9:58	N-GlyDE: a two-stage N-linked glycosylation site prediction incorporating gapped dipeptides and pattern-based encoding.	0:118	N-GlyDE: a two-stage N-linked glycosylation site prediction incorporating gapped dipeptides and pattern-based encoding.
31685900	0	68	gly	glycosylation	30:42	arg2	site					44:47	a two-stage N-linked glycosylation site prediction	9:58	N-GlyDE: a two-stage N-linked glycosylation site prediction incorporating gapped dipeptides and pattern-based encoding.	0:118	N-GlyDE: a two-stage N-linked glycosylation site prediction incorporating gapped dipeptides and pattern-based encoding.
31685900	6	69	theme	protein	749:755	arg1	similarity					757:766	a protein similarity	747:766	a protein similarity voting algorithm trained on both glycoproteins and non-glycoproteins	747:835	The first stage uses a protein similarity voting algorithm trained on both glycoproteins and non-glycoproteins to predict a score for a protein to improve glycosite prediction.
31685900	0	70	theme	gapped	74:79	arg1	dipeptides					81:90	gapped dipeptides	74:90	gapped dipeptides	74:90	N-GlyDE: a two-stage N-linked glycosylation site prediction incorporating gapped dipeptides and pattern-based encoding.
31685900	4	71	theme	protein	483:489	arg1	sequences					491:499	protein sequences	483:499	protein sequences	483:499	Most of them evaluate their performance at every asparagine in protein sequences, not confined to asparagine in the N-X-S/T sequon.
31685900	10	72	theme	N-GlyDE	1474:1480	arg1	available					1496:1504	available	1496:1504	available	1496:1504	The N-GlyDE web server is available at http://bioapp.iis.sinica.edu.tw/N-GlyDE/ .
31685900	10	72	theme	N-GlyDE	1474:1480	arg1	server					1486:1491	The N-GlyDE web server	1470:1491	The N-GlyDE web server	1470:1491	The N-GlyDE web server is available at http://bioapp.iis.sinica.edu.tw/N-GlyDE/ .
31685900	7	73	theme	N-linked	961:968	arg1	glycosites					970:979	N-linked glycosites	961:979	N-linked glycosites	961:979	The second stage uses a support vector machine to predict N-linked glycosites by utilizing features of gapped dipeptides, pattern-based predicted surface accessibility, and predicted secondary structure.
31685900	9	74	theme	dataset	1301:1307	arg1	sequons					1275:1281	N-X-S/T sequons	1267:1281	N-X-S/T sequons of an independent dataset comprised of 53 glycoproteins and 33 non-glycoproteins	1267:1362	Evaluated on N-X-S/T sequons of an independent dataset comprised of 53 glycoproteins and 33 non-glycoproteins, N-GlyDE achieves an accuracy and MCC of 0.740 and 0.499, respectively, outperforming the compared tools.
31685900	1	75	link	N-linked	120:127	arg1	glycosylation					129:141	N-linked glycosylation	120:141	N-linked glycosylation	120:141	N-linked glycosylation is one of the predominant post-translational modifications involved in a number of biological functions.
31685900	2	76	theme	glycosites	287:296	arg1	characterization					267:282	experimental characterization	254:282	experimental characterization of glycosites	254:296	Since experimental characterization of glycosites is challenging, glycosite prediction is crucial.
31685900	7	77	theme	surface	1049:1055	arg1	accessibility					1057:1069	pattern-based predicted surface accessibility	1025:1069	pattern-based predicted surface accessibility	1025:1069	The second stage uses a support vector machine to predict N-linked glycosites by utilizing features of gapped dipeptides, pattern-based predicted surface accessibility, and predicted secondary structure.
31685900	1	78	theme	predominant	157:167	arg1	modifications					188:200	the predominant post-translational modifications	153:200	the predominant post-translational modifications involved in a number of biological functions	153:245	N-linked glycosylation is one of the predominant post-translational modifications involved in a number of biological functions.
31685900	8	79	theme	results	1203:1209	arg1	adjustment					1161:1170	a weight adjustment	1152:1170	a weight adjustment of the second-stage prediction results based on the first-stage prediction score	1152:1251	N-GlyDE's final predictions are derived from a weight adjustment of the second-stage prediction results based on the first-stage prediction score.
29512823	7	0	from	decrease	1256:1263	arg1	charge					1276:1281	surface charge	1268:1281	surface charge	1268:1281	The analysis revealed evidence of unusual B-cell selection pressure and SHM-mediated decrease in surface charge and isoelectric point in ACPA.
29512823	7	0	from	decrease	1256:1263	arg1	ACPA					1308:1311	ACPA	1308:1311	ACPA	1308:1311	The analysis revealed evidence of unusual B-cell selection pressure and SHM-mediated decrease in surface charge and isoelectric point in ACPA.
29512823	5	1	contain	had	886:888	arg1	ACPA					875:878	ACPA	875:878	ACPA	875:878	When normalized for SHM, ACPA still had significantly higher frequency of N-linked motifs compared to all studied mAbs including highly mutated HIV broadly-neutralizing and malaria-associated mAbs.
29512823	5	1	contain	had	886:888	arg2	frequency					911:919	significantly higher frequency	890:919	significantly higher frequency of N-linked motifs	890:938	When normalized for SHM, ACPA still had significantly higher frequency of N-linked motifs compared to all studied mAbs including highly mutated HIV broadly-neutralizing and malaria-associated mAbs.
29512823	3	2	theme	predicted	487:495	arg1	motifs					520:525	predicted N-linked glycosylation motifs	487:525	predicted N-linked glycosylation motifs in silico	487:535	A total of 14 ACPA mAbs were evaluated for predicted N-linked glycosylation motifs in silico, and compared to 452 highly-mutated mAbs from RA patients and controls.
29512823	0	3	link	N-linked	16:23	arg1	features					75:82	key features	71:82	key features of monoclonal ACPA: Implications for B-cell selection	71:136	Variable domain N-linked glycosylation and negative surface charge are key features of monoclonal ACPA: Implications for B-cell selection.
29512823	0	3	link	N-linked	16:23	arg1	glycosylation					25:37	Variable domain N-linked glycosylation	0:37	Variable domain N-linked glycosylation	0:37	Variable domain N-linked glycosylation and negative surface charge are key features of monoclonal ACPA: Implications for B-cell selection.
29512823	0	3	link	N-linked	16:23	arg1	charge					60:65	negative surface charge	43:65	negative surface charge	43:65	Variable domain N-linked glycosylation and negative surface charge are key features of monoclonal ACPA: Implications for B-cell selection.
29512823	1	4	contain	have	160:163	arg1	cells					154:158	Autoreactive B cells	139:158	Autoreactive B cells	139:158	Autoreactive B cells have a central role in the pathogenesis of rheumatoid arthritis (RA), and recent findings have proposed that anti-citrullinated protein autoantibodies (ACPA) may be directly pathogenic.
29512823	1	4	contain	have	160:163	arg2	role					175:178	a central role	165:178	a central role	165:178	Autoreactive B cells have a central role in the pathogenesis of rheumatoid arthritis (RA), and recent findings have proposed that anti-citrullinated protein autoantibodies (ACPA) may be directly pathogenic.
29512823	1	5	theme	central	167:173	arg1	role					175:178	a central role	165:178	a central role	165:178	Autoreactive B cells have a central role in the pathogenesis of rheumatoid arthritis (RA), and recent findings have proposed that anti-citrullinated protein autoantibodies (ACPA) may be directly pathogenic.
29512823	4	6	theme	plasma	787:792	arg1	cells					794:798	synovial plasma cells	778:798	synovial plasma cells from seropositive (39%) and seronegative RA (7%)	778:847	Variable region N-linked motifs (N-X-S/T) were strikingly prevalent within ACPA (100%) compared to somatically hypermutated (SHM) RA bone marrow plasma cells (21%), and synovial plasma cells from seropositive (39%) and seronegative RA (7%).
29512823	6	7	theme	ACPA-mAbs	1067:1075	arg1	glycans					1056:1062	The Fab glycans	1048:1062	The Fab glycans of ACPA-mAbs	1048:1075	The Fab glycans of ACPA-mAbs were highly sialylated, contributed to altered charge, but did not influence antigen binding.
29512823	7	8	from	evidence	1193:1200	arg1	charge					1276:1281	surface charge	1268:1281	surface charge	1268:1281	The analysis revealed evidence of unusual B-cell selection pressure and SHM-mediated decrease in surface charge and isoelectric point in ACPA.
29512823	7	8	from	evidence	1193:1200	arg1	ACPA					1308:1311	ACPA	1308:1311	ACPA	1308:1311	The analysis revealed evidence of unusual B-cell selection pressure and SHM-mediated decrease in surface charge and isoelectric point in ACPA.
29512823	4	9	theme	region	618:623	arg1	N-X-S/T					642:648	N-X-S/T	642:648	N-X-S/T	642:648	Variable region N-linked motifs (N-X-S/T) were strikingly prevalent within ACPA (100%) compared to somatically hypermutated (SHM) RA bone marrow plasma cells (21%), and synovial plasma cells from seropositive (39%) and seronegative RA (7%).
29512823	4	9	theme	region	618:623	arg1	motifs					634:639	Variable region N-linked motifs	609:639	Variable region N-linked motifs (N-X-S/T)	609:649	Variable region N-linked motifs (N-X-S/T) were strikingly prevalent within ACPA (100%) compared to somatically hypermutated (SHM) RA bone marrow plasma cells (21%), and synovial plasma cells from seropositive (39%) and seronegative RA (7%).
29512823	5	10	theme	studied	956:962	arg1	broadly-neutralizing					998:1017	highly mutated HIV broadly-neutralizing	979:1017	highly mutated HIV broadly-neutralizing	979:1017	When normalized for SHM, ACPA still had significantly higher frequency of N-linked motifs compared to all studied mAbs including highly mutated HIV broadly-neutralizing and malaria-associated mAbs.
29512823	5	10	theme	studied	956:962	arg1	mAbs					964:967	all studied mAbs	952:967	all studied mAbs including highly mutated HIV broadly-neutralizing and malaria-associated mAbs	952:1045	When normalized for SHM, ACPA still had significantly higher frequency of N-linked motifs compared to all studied mAbs including highly mutated HIV broadly-neutralizing and malaria-associated mAbs.
29512823	5	10	theme	studied	956:962	arg1	mAbs					1042:1045	malaria-associated mAbs	1023:1045	malaria-associated mAbs	1023:1045	When normalized for SHM, ACPA still had significantly higher frequency of N-linked motifs compared to all studied mAbs including highly mutated HIV broadly-neutralizing and malaria-associated mAbs.
29512823	3	11	link	N-linked	497:504	arg1	motifs					520:525	predicted N-linked glycosylation motifs	487:525	predicted N-linked glycosylation motifs in silico	487:535	A total of 14 ACPA mAbs were evaluated for predicted N-linked glycosylation motifs in silico, and compared to 452 highly-mutated mAbs from RA patients and controls.
29512823	4	12	from	RA	841:842	arg1	cells					761:765	somatically hypermutated (SHM) RA bone marrow plasma cells	708:765	somatically hypermutated (SHM) RA bone marrow plasma cells (21%)	708:771	Variable region N-linked motifs (N-X-S/T) were strikingly prevalent within ACPA (100%) compared to somatically hypermutated (SHM) RA bone marrow plasma cells (21%), and synovial plasma cells from seropositive (39%) and seronegative RA (7%).
29512823	4	12	from	RA	841:842	arg1	%					770:770	21%	768:770	21%	768:770	Variable region N-linked motifs (N-X-S/T) were strikingly prevalent within ACPA (100%) compared to somatically hypermutated (SHM) RA bone marrow plasma cells (21%), and synovial plasma cells from seropositive (39%) and seronegative RA (7%).
29512823	4	12	from	RA	841:842	arg1	cells					794:798	synovial plasma cells	778:798	synovial plasma cells from seropositive (39%) and seronegative RA (7%)	778:847	Variable region N-linked motifs (N-X-S/T) were strikingly prevalent within ACPA (100%) compared to somatically hypermutated (SHM) RA bone marrow plasma cells (21%), and synovial plasma cells from seropositive (39%) and seronegative RA (7%).
29512823	3	13	theme	RA	583:584	arg1	patients					586:593	RA patients	583:593	RA patients	583:593	A total of 14 ACPA mAbs were evaluated for predicted N-linked glycosylation motifs in silico, and compared to 452 highly-mutated mAbs from RA patients and controls.
29512823	1	14	theme	rheumatoid	203:212	arg1	RA					225:226	RA	225:226	RA	225:226	Autoreactive B cells have a central role in the pathogenesis of rheumatoid arthritis (RA), and recent findings have proposed that anti-citrullinated protein autoantibodies (ACPA) may be directly pathogenic.
29512823	1	14	theme	rheumatoid	203:212	arg1	arthritis					214:222	rheumatoid arthritis	203:222	rheumatoid arthritis (RA)	203:227	Autoreactive B cells have a central role in the pathogenesis of rheumatoid arthritis (RA), and recent findings have proposed that anti-citrullinated protein autoantibodies (ACPA) may be directly pathogenic.
29512823	8	15	theme	anti-citrulline	1365:1379	arg1	immunity					1381:1388	anti-citrulline immunity	1365:1388	anti-citrulline immunity	1365:1388	It is still unknown how these distinct features of anti-citrulline immunity may have an impact on pathogenesis.
29512823	7	16	theme	isoelectric	1287:1297	arg1	point					1299:1303	isoelectric point	1287:1303	isoelectric point in ACPA	1287:1311	The analysis revealed evidence of unusual B-cell selection pressure and SHM-mediated decrease in surface charge and isoelectric point in ACPA.
29512823	5	17	theme	motifs	933:938	arg1	frequency					911:919	significantly higher frequency	890:919	significantly higher frequency of N-linked motifs	890:938	When normalized for SHM, ACPA still had significantly higher frequency of N-linked motifs compared to all studied mAbs including highly mutated HIV broadly-neutralizing and malaria-associated mAbs.
29512823	5	18	theme	malaria-associated	1023:1040	arg1	mAbs					1042:1045	malaria-associated mAbs	1023:1045	malaria-associated mAbs	1023:1045	When normalized for SHM, ACPA still had significantly higher frequency of N-linked motifs compared to all studied mAbs including highly mutated HIV broadly-neutralizing and malaria-associated mAbs.
29512823	1	19	theme	B	152:152	arg1	cells					154:158	Autoreactive B cells	139:158	Autoreactive B cells	139:158	Autoreactive B cells have a central role in the pathogenesis of rheumatoid arthritis (RA), and recent findings have proposed that anti-citrullinated protein autoantibodies (ACPA) may be directly pathogenic.
29512823	4	20	link	N-linked	625:632	arg1	N-X-S/T					642:648	N-X-S/T	642:648	N-X-S/T	642:648	Variable region N-linked motifs (N-X-S/T) were strikingly prevalent within ACPA (100%) compared to somatically hypermutated (SHM) RA bone marrow plasma cells (21%), and synovial plasma cells from seropositive (39%) and seronegative RA (7%).
29512823	4	20	link	N-linked	625:632	arg1	motifs					634:639	Variable region N-linked motifs	609:639	Variable region N-linked motifs (N-X-S/T)	609:649	Variable region N-linked motifs (N-X-S/T) were strikingly prevalent within ACPA (100%) compared to somatically hypermutated (SHM) RA bone marrow plasma cells (21%), and synovial plasma cells from seropositive (39%) and seronegative RA (7%).
29512823	3	21	gly	glycosylation	506:518	arg2	motifs					520:525	predicted N-linked glycosylation motifs	487:525	predicted N-linked glycosylation motifs in silico	487:535	A total of 14 ACPA mAbs were evaluated for predicted N-linked glycosylation motifs in silico, and compared to 452 highly-mutated mAbs from RA patients and controls.
29512823	0	22	theme	monoclonal	87:96	arg1	ACPA					98:101	monoclonal ACPA	87:101	monoclonal ACPA	87:101	Variable domain N-linked glycosylation and negative surface charge are key features of monoclonal ACPA: Implications for B-cell selection.
29512823	5	23	theme	mutated	986:992	arg1	broadly-neutralizing					998:1017	highly mutated HIV broadly-neutralizing	979:1017	highly mutated HIV broadly-neutralizing	979:1017	When normalized for SHM, ACPA still had significantly higher frequency of N-linked motifs compared to all studied mAbs including highly mutated HIV broadly-neutralizing and malaria-associated mAbs.
29512823	4	24	theme	RA	739:740	arg1	cells					761:765	somatically hypermutated (SHM) RA bone marrow plasma cells	708:765	somatically hypermutated (SHM) RA bone marrow plasma cells (21%)	708:771	Variable region N-linked motifs (N-X-S/T) were strikingly prevalent within ACPA (100%) compared to somatically hypermutated (SHM) RA bone marrow plasma cells (21%), and synovial plasma cells from seropositive (39%) and seronegative RA (7%).
29512823	4	24	theme	RA	739:740	arg1	%					770:770	21%	768:770	21%	768:770	Variable region N-linked motifs (N-X-S/T) were strikingly prevalent within ACPA (100%) compared to somatically hypermutated (SHM) RA bone marrow plasma cells (21%), and synovial plasma cells from seropositive (39%) and seronegative RA (7%).
29512823	9	25	theme	selective	1465:1473	arg1	advantages					1475:1484	selective advantages	1465:1484	selective advantages for ACPA+ B cells	1465:1502	However, it is evident that they offer selective advantages for ACPA+ B cells, possibly through non-antigen driven mechanisms.
29512823	0	26	theme	Variable	0:7	arg1	features					75:82	key features	71:82	key features of monoclonal ACPA: Implications for B-cell selection	71:136	Variable domain N-linked glycosylation and negative surface charge are key features of monoclonal ACPA: Implications for B-cell selection.
29512823	0	26	theme	Variable	0:7	arg1	glycosylation					25:37	Variable domain N-linked glycosylation	0:37	Variable domain N-linked glycosylation	0:37	Variable domain N-linked glycosylation and negative surface charge are key features of monoclonal ACPA: Implications for B-cell selection.
29512823	0	26	theme	Variable	0:7	arg1	charge					60:65	negative surface charge	43:65	negative surface charge	43:65	Variable domain N-linked glycosylation and negative surface charge are key features of monoclonal ACPA: Implications for B-cell selection.
29512823	0	27	dep	features	75:82	arg1	Implications					104:115	Implications	104:115	key features of monoclonal ACPA: Implications for B-cell selection	71:136	Variable domain N-linked glycosylation and negative surface charge are key features of monoclonal ACPA: Implications for B-cell selection.
29512823	2	28	theme	cloned	431:436	arg1	mAbs					438:441	single-cell cloned mAbs	419:441	single-cell cloned mAbs	419:441	Herein, we demonstrate the frequency of variable-region glycosylation in single-cell cloned mAbs.
29512823	4	29	theme	hypermutated	720:731	arg1	cells					761:765	somatically hypermutated (SHM) RA bone marrow plasma cells	708:765	somatically hypermutated (SHM) RA bone marrow plasma cells (21%)	708:771	Variable region N-linked motifs (N-X-S/T) were strikingly prevalent within ACPA (100%) compared to somatically hypermutated (SHM) RA bone marrow plasma cells (21%), and synovial plasma cells from seropositive (39%) and seronegative RA (7%).
29512823	4	29	theme	hypermutated	720:731	arg1	%					770:770	21%	768:770	21%	768:770	Variable region N-linked motifs (N-X-S/T) were strikingly prevalent within ACPA (100%) compared to somatically hypermutated (SHM) RA bone marrow plasma cells (21%), and synovial plasma cells from seropositive (39%) and seronegative RA (7%).
29512823	0	30	theme	N-linked	16:23	arg1	features					75:82	key features	71:82	key features of monoclonal ACPA: Implications for B-cell selection	71:136	Variable domain N-linked glycosylation and negative surface charge are key features of monoclonal ACPA: Implications for B-cell selection.
29512823	0	30	theme	N-linked	16:23	arg1	glycosylation					25:37	Variable domain N-linked glycosylation	0:37	Variable domain N-linked glycosylation	0:37	Variable domain N-linked glycosylation and negative surface charge are key features of monoclonal ACPA: Implications for B-cell selection.
29512823	0	30	theme	N-linked	16:23	arg1	charge					60:65	negative surface charge	43:65	negative surface charge	43:65	Variable domain N-linked glycosylation and negative surface charge are key features of monoclonal ACPA: Implications for B-cell selection.
29512823	9	31	theme	B	1496:1496	arg1	cells					1498:1502	ACPA+ B cells	1490:1502	ACPA+ B cells	1490:1502	However, it is evident that they offer selective advantages for ACPA+ B cells, possibly through non-antigen driven mechanisms.
29512823	7	32	theme	selection	1220:1228	arg1	pressure					1230:1237	unusual B-cell selection pressure	1205:1237	unusual B-cell selection pressure	1205:1237	The analysis revealed evidence of unusual B-cell selection pressure and SHM-mediated decrease in surface charge and isoelectric point in ACPA.
29512823	2	33	theme	variable-region	386:400	arg1	glycosylation					402:414	variable-region glycosylation	386:414	variable-region glycosylation	386:414	Herein, we demonstrate the frequency of variable-region glycosylation in single-cell cloned mAbs.
29512823	1	34	theme	protein	288:294	arg1	autoantibodies					296:309	anti-citrullinated protein autoantibodies	269:309	anti-citrullinated protein autoantibodies (ACPA)	269:316	Autoreactive B cells have a central role in the pathogenesis of rheumatoid arthritis (RA), and recent findings have proposed that anti-citrullinated protein autoantibodies (ACPA) may be directly pathogenic.
29512823	1	34	theme	protein	288:294	arg1	ACPA					312:315	ACPA	312:315	ACPA	312:315	Autoreactive B cells have a central role in the pathogenesis of rheumatoid arthritis (RA), and recent findings have proposed that anti-citrullinated protein autoantibodies (ACPA) may be directly pathogenic.
29512823	7	35	theme	unusual	1205:1211	arg1	pressure					1230:1237	unusual B-cell selection pressure	1205:1237	unusual B-cell selection pressure	1205:1237	The analysis revealed evidence of unusual B-cell selection pressure and SHM-mediated decrease in surface charge and isoelectric point in ACPA.
29512823	0	36	theme	surface	52:58	arg1	features					75:82	key features	71:82	key features of monoclonal ACPA: Implications for B-cell selection	71:136	Variable domain N-linked glycosylation and negative surface charge are key features of monoclonal ACPA: Implications for B-cell selection.
29512823	0	36	theme	surface	52:58	arg1	glycosylation					25:37	Variable domain N-linked glycosylation	0:37	Variable domain N-linked glycosylation	0:37	Variable domain N-linked glycosylation and negative surface charge are key features of monoclonal ACPA: Implications for B-cell selection.
29512823	0	36	theme	surface	52:58	arg1	charge					60:65	negative surface charge	43:65	negative surface charge	43:65	Variable domain N-linked glycosylation and negative surface charge are key features of monoclonal ACPA: Implications for B-cell selection.
29512823	5	37	theme	N-linked	924:931	arg1	motifs					933:938	N-linked motifs	924:938	N-linked motifs	924:938	When normalized for SHM, ACPA still had significantly higher frequency of N-linked motifs compared to all studied mAbs including highly mutated HIV broadly-neutralizing and malaria-associated mAbs.
29512823	2	38	from	frequency	373:381	arg1	mAbs					438:441	single-cell cloned mAbs	419:441	single-cell cloned mAbs	419:441	Herein, we demonstrate the frequency of variable-region glycosylation in single-cell cloned mAbs.
29512823	4	39	theme	marrow	747:752	arg1	cells					761:765	somatically hypermutated (SHM) RA bone marrow plasma cells	708:765	somatically hypermutated (SHM) RA bone marrow plasma cells (21%)	708:771	Variable region N-linked motifs (N-X-S/T) were strikingly prevalent within ACPA (100%) compared to somatically hypermutated (SHM) RA bone marrow plasma cells (21%), and synovial plasma cells from seropositive (39%) and seronegative RA (7%).
29512823	4	39	theme	marrow	747:752	arg1	%					770:770	21%	768:770	21%	768:770	Variable region N-linked motifs (N-X-S/T) were strikingly prevalent within ACPA (100%) compared to somatically hypermutated (SHM) RA bone marrow plasma cells (21%), and synovial plasma cells from seropositive (39%) and seronegative RA (7%).
29512823	3	40	theme	ACPA	458:461	arg1	mAbs					463:466	14 ACPA mAbs	455:466	14 ACPA mAbs	455:466	A total of 14 ACPA mAbs were evaluated for predicted N-linked glycosylation motifs in silico, and compared to 452 highly-mutated mAbs from RA patients and controls.
29512823	1	41	theme	arthritis	214:222	arg1	pathogenesis					187:198	the pathogenesis	183:198	the pathogenesis of rheumatoid arthritis (RA)	183:227	Autoreactive B cells have a central role in the pathogenesis of rheumatoid arthritis (RA), and recent findings have proposed that anti-citrullinated protein autoantibodies (ACPA) may be directly pathogenic.
29512823	9	42	theme	driven	1534:1539	arg1	mechanisms					1541:1550	non-antigen driven mechanisms	1522:1550	non-antigen driven mechanisms	1522:1550	However, it is evident that they offer selective advantages for ACPA+ B cells, possibly through non-antigen driven mechanisms.
29512823	8	43	theme	distinct	1344:1351	arg1	features					1353:1360	these distinct features	1338:1360	these distinct features of anti-citrulline immunity	1338:1388	It is still unknown how these distinct features of anti-citrulline immunity may have an impact on pathogenesis.
29512823	3	44	theme	N-linked	497:504	arg1	motifs					520:525	predicted N-linked glycosylation motifs	487:525	predicted N-linked glycosylation motifs in silico	487:535	A total of 14 ACPA mAbs were evaluated for predicted N-linked glycosylation motifs in silico, and compared to 452 highly-mutated mAbs from RA patients and controls.
29512823	1	45	theme	recent	234:239	arg1	findings					241:248	recent findings	234:248	recent findings	234:248	Autoreactive B cells have a central role in the pathogenesis of rheumatoid arthritis (RA), and recent findings have proposed that anti-citrullinated protein autoantibodies (ACPA) may be directly pathogenic.
29512823	6	46	theme	antigen	1154:1160	arg1	binding					1162:1168	antigen binding	1154:1168	antigen binding	1154:1168	The Fab glycans of ACPA-mAbs were highly sialylated, contributed to altered charge, but did not influence antigen binding.
29512823	4	47	theme	synovial	778:785	arg1	cells					794:798	synovial plasma cells	778:798	synovial plasma cells from seropositive (39%) and seronegative RA (7%)	778:847	Variable region N-linked motifs (N-X-S/T) were strikingly prevalent within ACPA (100%) compared to somatically hypermutated (SHM) RA bone marrow plasma cells (21%), and synovial plasma cells from seropositive (39%) and seronegative RA (7%).
29512823	0	48	theme	B-cell	121:126	arg1	selection					128:136	B-cell selection	121:136	B-cell selection	121:136	Variable domain N-linked glycosylation and negative surface charge are key features of monoclonal ACPA: Implications for B-cell selection.
29512823	4	49	theme	seronegative	828:839	arg1	RA					841:842	seronegative RA	828:842	seronegative RA (7%)	828:847	Variable region N-linked motifs (N-X-S/T) were strikingly prevalent within ACPA (100%) compared to somatically hypermutated (SHM) RA bone marrow plasma cells (21%), and synovial plasma cells from seropositive (39%) and seronegative RA (7%).
29512823	4	49	theme	seronegative	828:839	arg1	%					846:846	7%	845:846	7%	845:846	Variable region N-linked motifs (N-X-S/T) were strikingly prevalent within ACPA (100%) compared to somatically hypermutated (SHM) RA bone marrow plasma cells (21%), and synovial plasma cells from seropositive (39%) and seronegative RA (7%).
29512823	6	50	theme	Fab	1052:1054	arg1	glycans					1056:1062	The Fab glycans	1048:1062	The Fab glycans of ACPA-mAbs	1048:1075	The Fab glycans of ACPA-mAbs were highly sialylated, contributed to altered charge, but did not influence antigen binding.
29512823	4	51	theme	Variable	609:616	arg1	N-X-S/T					642:648	N-X-S/T	642:648	N-X-S/T	642:648	Variable region N-linked motifs (N-X-S/T) were strikingly prevalent within ACPA (100%) compared to somatically hypermutated (SHM) RA bone marrow plasma cells (21%), and synovial plasma cells from seropositive (39%) and seronegative RA (7%).
29512823	4	51	theme	Variable	609:616	arg1	motifs					634:639	Variable region N-linked motifs	609:639	Variable region N-linked motifs (N-X-S/T)	609:649	Variable region N-linked motifs (N-X-S/T) were strikingly prevalent within ACPA (100%) compared to somatically hypermutated (SHM) RA bone marrow plasma cells (21%), and synovial plasma cells from seropositive (39%) and seronegative RA (7%).
29512823	6	52	gly	sialylated	1089:1098	arg1	glycans					1056:1062	The Fab glycans	1048:1062	The Fab glycans of ACPA-mAbs	1048:1075	The Fab glycans of ACPA-mAbs were highly sialylated, contributed to altered charge, but did not influence antigen binding.
29512823	5	53	link	N-linked	924:931	arg1	motifs					933:938	N-linked motifs	924:938	N-linked motifs	924:938	When normalized for SHM, ACPA still had significantly higher frequency of N-linked motifs compared to all studied mAbs including highly mutated HIV broadly-neutralizing and malaria-associated mAbs.
29512823	8	54	theme	immunity	1381:1388	arg1	features					1353:1360	these distinct features	1338:1360	these distinct features of anti-citrulline immunity	1338:1388	It is still unknown how these distinct features of anti-citrulline immunity may have an impact on pathogenesis.
29512823	4	55	theme	N-linked	625:632	arg1	N-X-S/T					642:648	N-X-S/T	642:648	N-X-S/T	642:648	Variable region N-linked motifs (N-X-S/T) were strikingly prevalent within ACPA (100%) compared to somatically hypermutated (SHM) RA bone marrow plasma cells (21%), and synovial plasma cells from seropositive (39%) and seronegative RA (7%).
29512823	4	55	theme	N-linked	625:632	arg1	motifs					634:639	Variable region N-linked motifs	609:639	Variable region N-linked motifs (N-X-S/T)	609:649	Variable region N-linked motifs (N-X-S/T) were strikingly prevalent within ACPA (100%) compared to somatically hypermutated (SHM) RA bone marrow plasma cells (21%), and synovial plasma cells from seropositive (39%) and seronegative RA (7%).
29512823	3	56	theme	highly-mutated	558:571	arg1	mAbs					573:576	452 highly-mutated mAbs	554:576	452 highly-mutated mAbs from RA patients and controls	554:606	A total of 14 ACPA mAbs were evaluated for predicted N-linked glycosylation motifs in silico, and compared to 452 highly-mutated mAbs from RA patients and controls.
29512823	4	57	dep	hypermutated	720:731	arg1	SHM					734:736	SHM	734:736	SHM	734:736	Variable region N-linked motifs (N-X-S/T) were strikingly prevalent within ACPA (100%) compared to somatically hypermutated (SHM) RA bone marrow plasma cells (21%), and synovial plasma cells from seropositive (39%) and seronegative RA (7%).
29512823	6	58	theme	altered	1116:1122	arg1	charge					1124:1129	altered charge	1116:1129	altered charge	1116:1129	The Fab glycans of ACPA-mAbs were highly sialylated, contributed to altered charge, but did not influence antigen binding.
29512823	0	59	theme	key	71:73	arg1	features					75:82	key features	71:82	key features of monoclonal ACPA: Implications for B-cell selection	71:136	Variable domain N-linked glycosylation and negative surface charge are key features of monoclonal ACPA: Implications for B-cell selection.
29512823	0	59	theme	key	71:73	arg1	glycosylation					25:37	Variable domain N-linked glycosylation	0:37	Variable domain N-linked glycosylation	0:37	Variable domain N-linked glycosylation and negative surface charge are key features of monoclonal ACPA: Implications for B-cell selection.
29512823	0	59	theme	key	71:73	arg1	charge					60:65	negative surface charge	43:65	negative surface charge	43:65	Variable domain N-linked glycosylation and negative surface charge are key features of monoclonal ACPA: Implications for B-cell selection.
29512823	1	60	theme	Autoreactive	139:150	arg1	cells					154:158	Autoreactive B cells	139:158	Autoreactive B cells	139:158	Autoreactive B cells have a central role in the pathogenesis of rheumatoid arthritis (RA), and recent findings have proposed that anti-citrullinated protein autoantibodies (ACPA) may be directly pathogenic.
29512823	7	61	theme	surface	1268:1274	arg1	charge					1276:1281	surface charge	1268:1281	surface charge	1268:1281	The analysis revealed evidence of unusual B-cell selection pressure and SHM-mediated decrease in surface charge and isoelectric point in ACPA.
29512823	3	62	from	motifs	520:525	arg1	silico					530:535	silico	530:535	silico	530:535	A total of 14 ACPA mAbs were evaluated for predicted N-linked glycosylation motifs in silico, and compared to 452 highly-mutated mAbs from RA patients and controls.
29512823	4	63	from	seropositive	805:816	arg1	cells					761:765	somatically hypermutated (SHM) RA bone marrow plasma cells	708:765	somatically hypermutated (SHM) RA bone marrow plasma cells (21%)	708:771	Variable region N-linked motifs (N-X-S/T) were strikingly prevalent within ACPA (100%) compared to somatically hypermutated (SHM) RA bone marrow plasma cells (21%), and synovial plasma cells from seropositive (39%) and seronegative RA (7%).
29512823	4	63	from	seropositive	805:816	arg1	%					770:770	21%	768:770	21%	768:770	Variable region N-linked motifs (N-X-S/T) were strikingly prevalent within ACPA (100%) compared to somatically hypermutated (SHM) RA bone marrow plasma cells (21%), and synovial plasma cells from seropositive (39%) and seronegative RA (7%).
29512823	4	63	from	seropositive	805:816	arg1	cells					794:798	synovial plasma cells	778:798	synovial plasma cells from seropositive (39%) and seronegative RA (7%)	778:847	Variable region N-linked motifs (N-X-S/T) were strikingly prevalent within ACPA (100%) compared to somatically hypermutated (SHM) RA bone marrow plasma cells (21%), and synovial plasma cells from seropositive (39%) and seronegative RA (7%).
29512823	0	64	theme	ACPA	98:101	arg1	features					75:82	key features	71:82	key features of monoclonal ACPA: Implications for B-cell selection	71:136	Variable domain N-linked glycosylation and negative surface charge are key features of monoclonal ACPA: Implications for B-cell selection.
29512823	0	64	theme	ACPA	98:101	arg1	glycosylation					25:37	Variable domain N-linked glycosylation	0:37	Variable domain N-linked glycosylation	0:37	Variable domain N-linked glycosylation and negative surface charge are key features of monoclonal ACPA: Implications for B-cell selection.
29512823	0	64	theme	ACPA	98:101	arg1	charge					60:65	negative surface charge	43:65	negative surface charge	43:65	Variable domain N-linked glycosylation and negative surface charge are key features of monoclonal ACPA: Implications for B-cell selection.
29512823	3	65	from	patients	586:593	arg1	mAbs					573:576	452 highly-mutated mAbs	554:576	452 highly-mutated mAbs from RA patients and controls	554:606	A total of 14 ACPA mAbs were evaluated for predicted N-linked glycosylation motifs in silico, and compared to 452 highly-mutated mAbs from RA patients and controls.
29512823	5	66	theme	HIV	994:996	arg1	broadly-neutralizing					998:1017	highly mutated HIV broadly-neutralizing	979:1017	highly mutated HIV broadly-neutralizing	979:1017	When normalized for SHM, ACPA still had significantly higher frequency of N-linked motifs compared to all studied mAbs including highly mutated HIV broadly-neutralizing and malaria-associated mAbs.
29512823	4	67	dep	prevalent	667:675	arg1	%					693:693	100%	690:693	100%	690:693	Variable region N-linked motifs (N-X-S/T) were strikingly prevalent within ACPA (100%) compared to somatically hypermutated (SHM) RA bone marrow plasma cells (21%), and synovial plasma cells from seropositive (39%) and seronegative RA (7%).
29512823	7	68	theme	SHM-mediated	1243:1254	arg1	decrease					1256:1263	SHM-mediated decrease	1243:1263	SHM-mediated decrease in surface charge	1243:1281	The analysis revealed evidence of unusual B-cell selection pressure and SHM-mediated decrease in surface charge and isoelectric point in ACPA.
29512823	3	69	from	controls	599:606	arg1	mAbs					573:576	452 highly-mutated mAbs	554:576	452 highly-mutated mAbs from RA patients and controls	554:606	A total of 14 ACPA mAbs were evaluated for predicted N-linked glycosylation motifs in silico, and compared to 452 highly-mutated mAbs from RA patients and controls.
29512823	0	70	theme	domain	9:14	arg1	features					75:82	key features	71:82	key features of monoclonal ACPA: Implications for B-cell selection	71:136	Variable domain N-linked glycosylation and negative surface charge are key features of monoclonal ACPA: Implications for B-cell selection.
29512823	0	70	theme	domain	9:14	arg1	glycosylation					25:37	Variable domain N-linked glycosylation	0:37	Variable domain N-linked glycosylation	0:37	Variable domain N-linked glycosylation and negative surface charge are key features of monoclonal ACPA: Implications for B-cell selection.
29512823	0	70	theme	domain	9:14	arg1	charge					60:65	negative surface charge	43:65	negative surface charge	43:65	Variable domain N-linked glycosylation and negative surface charge are key features of monoclonal ACPA: Implications for B-cell selection.
29512823	7	71	theme	pressure	1230:1237	arg1	point					1299:1303	isoelectric point	1287:1303	isoelectric point in ACPA	1287:1311	The analysis revealed evidence of unusual B-cell selection pressure and SHM-mediated decrease in surface charge and isoelectric point in ACPA.
29512823	7	71	theme	pressure	1230:1237	arg1	decrease					1256:1263	SHM-mediated decrease	1243:1263	SHM-mediated decrease in surface charge	1243:1281	The analysis revealed evidence of unusual B-cell selection pressure and SHM-mediated decrease in surface charge and isoelectric point in ACPA.
29512823	7	71	theme	pressure	1230:1237	arg1	evidence					1193:1200	evidence	1193:1200	evidence of unusual B-cell selection pressure	1193:1237	The analysis revealed evidence of unusual B-cell selection pressure and SHM-mediated decrease in surface charge and isoelectric point in ACPA.
29512823	9	72	theme	ACPA+	1490:1494	arg1	cells					1498:1502	ACPA+ B cells	1490:1502	ACPA+ B cells	1490:1502	However, it is evident that they offer selective advantages for ACPA+ B cells, possibly through non-antigen driven mechanisms.
29512823	7	73	from	point	1299:1303	arg1	charge					1276:1281	surface charge	1268:1281	surface charge	1268:1281	The analysis revealed evidence of unusual B-cell selection pressure and SHM-mediated decrease in surface charge and isoelectric point in ACPA.
29512823	7	73	from	point	1299:1303	arg1	ACPA					1308:1311	ACPA	1308:1311	ACPA	1308:1311	The analysis revealed evidence of unusual B-cell selection pressure and SHM-mediated decrease in surface charge and isoelectric point in ACPA.
29512823	7	74	theme	B-cell	1213:1218	arg1	pressure					1230:1237	unusual B-cell selection pressure	1205:1237	unusual B-cell selection pressure	1205:1237	The analysis revealed evidence of unusual B-cell selection pressure and SHM-mediated decrease in surface charge and isoelectric point in ACPA.
29512823	2	75	theme	single-cell	419:429	arg1	mAbs					438:441	single-cell cloned mAbs	419:441	single-cell cloned mAbs	419:441	Herein, we demonstrate the frequency of variable-region glycosylation in single-cell cloned mAbs.
29512823	2	76	theme	glycosylation	402:414	arg1	frequency					373:381	the frequency	369:381	the frequency of variable-region glycosylation in single-cell cloned mAbs	369:441	Herein, we demonstrate the frequency of variable-region glycosylation in single-cell cloned mAbs.
29512823	0	77	theme	negative	43:50	arg1	features					75:82	key features	71:82	key features of monoclonal ACPA: Implications for B-cell selection	71:136	Variable domain N-linked glycosylation and negative surface charge are key features of monoclonal ACPA: Implications for B-cell selection.
29512823	0	77	theme	negative	43:50	arg1	glycosylation					25:37	Variable domain N-linked glycosylation	0:37	Variable domain N-linked glycosylation	0:37	Variable domain N-linked glycosylation and negative surface charge are key features of monoclonal ACPA: Implications for B-cell selection.
29512823	0	77	theme	negative	43:50	arg1	charge					60:65	negative surface charge	43:65	negative surface charge	43:65	Variable domain N-linked glycosylation and negative surface charge are key features of monoclonal ACPA: Implications for B-cell selection.
29512823	1	78	theme	anti-citrullinated	269:286	arg1	autoantibodies					296:309	anti-citrullinated protein autoantibodies	269:309	anti-citrullinated protein autoantibodies (ACPA)	269:316	Autoreactive B cells have a central role in the pathogenesis of rheumatoid arthritis (RA), and recent findings have proposed that anti-citrullinated protein autoantibodies (ACPA) may be directly pathogenic.
29512823	1	78	theme	anti-citrullinated	269:286	arg1	ACPA					312:315	ACPA	312:315	ACPA	312:315	Autoreactive B cells have a central role in the pathogenesis of rheumatoid arthritis (RA), and recent findings have proposed that anti-citrullinated protein autoantibodies (ACPA) may be directly pathogenic.
29512823	5	79	theme	higher	904:909	arg1	frequency					911:919	significantly higher frequency	890:919	significantly higher frequency of N-linked motifs	890:938	When normalized for SHM, ACPA still had significantly higher frequency of N-linked motifs compared to all studied mAbs including highly mutated HIV broadly-neutralizing and malaria-associated mAbs.
29512823	9	80	theme	non-antigen	1522:1532	arg1	mechanisms					1541:1550	non-antigen driven mechanisms	1522:1550	non-antigen driven mechanisms	1522:1550	However, it is evident that they offer selective advantages for ACPA+ B cells, possibly through non-antigen driven mechanisms.
29512823	4	81	theme	bone	742:745	arg1	cells					761:765	somatically hypermutated (SHM) RA bone marrow plasma cells	708:765	somatically hypermutated (SHM) RA bone marrow plasma cells (21%)	708:771	Variable region N-linked motifs (N-X-S/T) were strikingly prevalent within ACPA (100%) compared to somatically hypermutated (SHM) RA bone marrow plasma cells (21%), and synovial plasma cells from seropositive (39%) and seronegative RA (7%).
29512823	4	81	theme	bone	742:745	arg1	%					770:770	21%	768:770	21%	768:770	Variable region N-linked motifs (N-X-S/T) were strikingly prevalent within ACPA (100%) compared to somatically hypermutated (SHM) RA bone marrow plasma cells (21%), and synovial plasma cells from seropositive (39%) and seronegative RA (7%).
29512823	3	82	theme	mAbs	463:466	arg1	total					446:450	A total	444:450	A total of 14 ACPA mAbs	444:466	A total of 14 ACPA mAbs were evaluated for predicted N-linked glycosylation motifs in silico, and compared to 452 highly-mutated mAbs from RA patients and controls.
29512823	8	83	contain	have	1394:1397	arg2	impact					1402:1407	an impact	1399:1407	an impact	1399:1407	It is still unknown how these distinct features of anti-citrulline immunity may have an impact on pathogenesis.
29512823	8	83	contain	have	1394:1397	arg1	features					1353:1360	these distinct features	1338:1360	these distinct features of anti-citrulline immunity	1338:1388	It is still unknown how these distinct features of anti-citrulline immunity may have an impact on pathogenesis.
29512823	4	84	theme	plasma	754:759	arg1	cells					761:765	somatically hypermutated (SHM) RA bone marrow plasma cells	708:765	somatically hypermutated (SHM) RA bone marrow plasma cells (21%)	708:771	Variable region N-linked motifs (N-X-S/T) were strikingly prevalent within ACPA (100%) compared to somatically hypermutated (SHM) RA bone marrow plasma cells (21%), and synovial plasma cells from seropositive (39%) and seronegative RA (7%).
29512823	4	84	theme	plasma	754:759	arg1	%					770:770	21%	768:770	21%	768:770	Variable region N-linked motifs (N-X-S/T) were strikingly prevalent within ACPA (100%) compared to somatically hypermutated (SHM) RA bone marrow plasma cells (21%), and synovial plasma cells from seropositive (39%) and seronegative RA (7%).
29512823	3	85	theme	glycosylation	506:518	arg1	motifs					520:525	predicted N-linked glycosylation motifs	487:525	predicted N-linked glycosylation motifs in silico	487:535	A total of 14 ACPA mAbs were evaluated for predicted N-linked glycosylation motifs in silico, and compared to 452 highly-mutated mAbs from RA patients and controls.
30523691	2	0	theme	peptide	587:593	arg1	spectra					604:610	the CID peptide fragment spectra	579:610	the CID peptide fragment spectra	579:610	Collision-induced dissociation (CID) spectra of O-fucosylated peptides cannot be sequenced by standard tandem mass spectrometry (MS/MS) sequence database search engines because O-linked glycans are highly labile in the gas phase and are effectively absent from the CID peptide fragment spectra, resulting in a large mass error.
30523691	5	1	theme	database	1156:1163	arg1	engine					1172:1177	the MS/MS sequence database search engine Comet and the MS data analysis suite Trans-Proteomic Pipeline	1137:1239	engine	1172:1177	In order to facilitate high-throughput, automatic identification of O-fucosylated peptides from CID spectra, we re-engineered the MS/MS sequence database search engine Comet and the MS data analysis suite Trans-Proteomic Pipeline to enable automated sequencing of peptides exhibiting the neutral losses characteristic of labile O-linked glycans.
30523691	3	2	link	O-linked	693:700	arg1	glycans					702:708	O-linked glycans	693:708	O-linked glycans on peptide fragments	693:729	Electron transfer dissociation (ETD) preserves O-linked glycans on peptide fragments, but only a subset of tryptic peptides with low m/ z can be reliably sequenced from ETD spectra compared to CID.
30523691	4	3	theme	mass	965:968	arg1	spectra					970:976	CID mass spectra	961:976	CID mass spectra	961:976	Accordingly, studies to date that have used MS to identify O-fucosylated TSRs have required manual interpretation of CID mass spectra even when ETD was also employed.
30523691	1	4	theme	small	164:168	arg1	repeats					148:154	Thrombospondin type 1 repeats	126:154	Thrombospondin type 1 repeats (TSRs)	126:161	Thrombospondin type 1 repeats (TSRs), small adhesive protein domains with a wide range of functions, are usually modified with O-linked fucose, which may be extended to O-fucose-β1,3-glucose.
30523691	1	4	theme	small	164:168	arg1	domains					187:193	small adhesive protein domains	164:193	small adhesive protein domains with a wide range of functions	164:224	Thrombospondin type 1 repeats (TSRs), small adhesive protein domains with a wide range of functions, are usually modified with O-linked fucose, which may be extended to O-fucose-β1,3-glucose.
30523691	5	5	gly	O-fucosylated	1079:1091	arg1	peptides					1093:1100	O-fucosylated peptides	1079:1100	O-fucosylated peptides from CID spectra	1079:1117	In order to facilitate high-throughput, automatic identification of O-fucosylated peptides from CID spectra, we re-engineered the MS/MS sequence database search engine Comet and the MS data analysis suite Trans-Proteomic Pipeline to enable automated sequencing of peptides exhibiting the neutral losses characteristic of labile O-linked glycans.
30523691	1	6	theme	protein	179:185	arg1	repeats					148:154	Thrombospondin type 1 repeats	126:154	Thrombospondin type 1 repeats (TSRs)	126:161	Thrombospondin type 1 repeats (TSRs), small adhesive protein domains with a wide range of functions, are usually modified with O-linked fucose, which may be extended to O-fucose-β1,3-glucose.
30523691	1	6	theme	protein	179:185	arg1	domains					187:193	small adhesive protein domains	164:193	small adhesive protein domains with a wide range of functions	164:224	Thrombospondin type 1 repeats (TSRs), small adhesive protein domains with a wide range of functions, are usually modified with O-linked fucose, which may be extended to O-fucose-β1,3-glucose.
30523691	5	7	dep	analysis	1201:1208	arg1	Pipeline					1232:1239	suite Trans-Proteomic Pipeline	1210:1239	the MS data analysis suite Trans-Proteomic Pipeline	1189:1239	In order to facilitate high-throughput, automatic identification of O-fucosylated peptides from CID spectra, we re-engineered the MS/MS sequence database search engine Comet and the MS data analysis suite Trans-Proteomic Pipeline to enable automated sequencing of peptides exhibiting the neutral losses characteristic of labile O-linked glycans.
30523691	3	8	theme	peptide	713:719	arg1	fragments					721:729	peptide fragments	713:729	peptide fragments	713:729	Electron transfer dissociation (ETD) preserves O-linked glycans on peptide fragments, but only a subset of tryptic peptides with low m/ z can be reliably sequenced from ETD spectra compared to CID.
30523691	1	9	theme	wide	202:205	arg1	range					207:211	a wide range	200:211	a wide range of functions	200:224	Thrombospondin type 1 repeats (TSRs), small adhesive protein domains with a wide range of functions, are usually modified with O-linked fucose, which may be extended to O-fucose-β1,3-glucose.
30523691	1	10	mod	modified	239:246	arg3	fucose					262:267	O-linked fucose	253:267	O-linked fucose	253:267	Thrombospondin type 1 repeats (TSRs), small adhesive protein domains with a wide range of functions, are usually modified with O-linked fucose, which may be extended to O-fucose-β1,3-glucose.
30523691	1	10	mod	modified	239:246	arg1	TSRs					157:160	TSRs	157:160	TSRs	157:160	Thrombospondin type 1 repeats (TSRs), small adhesive protein domains with a wide range of functions, are usually modified with O-linked fucose, which may be extended to O-fucose-β1,3-glucose.
30523691	1	10	mod	modified	239:246	arg1	domains					187:193	small adhesive protein domains	164:193	small adhesive protein domains with a wide range of functions	164:224	Thrombospondin type 1 repeats (TSRs), small adhesive protein domains with a wide range of functions, are usually modified with O-linked fucose, which may be extended to O-fucose-β1,3-glucose.
30523691	1	10	mod	modified	239:246	arg1	repeats					148:154	Thrombospondin type 1 repeats	126:154	Thrombospondin type 1 repeats (TSRs)	126:161	Thrombospondin type 1 repeats (TSRs), small adhesive protein domains with a wide range of functions, are usually modified with O-linked fucose, which may be extended to O-fucose-β1,3-glucose.
30523691	1	11	theme	Thrombospondin	126:139	arg1	TSRs					157:160	TSRs	157:160	TSRs	157:160	Thrombospondin type 1 repeats (TSRs), small adhesive protein domains with a wide range of functions, are usually modified with O-linked fucose, which may be extended to O-fucose-β1,3-glucose.
30523691	1	11	theme	Thrombospondin	126:139	arg1	domains					187:193	small adhesive protein domains	164:193	small adhesive protein domains with a wide range of functions	164:224	Thrombospondin type 1 repeats (TSRs), small adhesive protein domains with a wide range of functions, are usually modified with O-linked fucose, which may be extended to O-fucose-β1,3-glucose.
30523691	1	11	theme	Thrombospondin	126:139	arg1	repeats					148:154	Thrombospondin type 1 repeats	126:154	Thrombospondin type 1 repeats (TSRs)	126:161	Thrombospondin type 1 repeats (TSRs), small adhesive protein domains with a wide range of functions, are usually modified with O-linked fucose, which may be extended to O-fucose-β1,3-glucose.
30523691	5	12	dep	engine	1172:1177	arg1	Comet					1179:1183	Comet	1179:1183	Comet	1179:1183	In order to facilitate high-throughput, automatic identification of O-fucosylated peptides from CID spectra, we re-engineered the MS/MS sequence database search engine Comet and the MS data analysis suite Trans-Proteomic Pipeline to enable automated sequencing of peptides exhibiting the neutral losses characteristic of labile O-linked glycans.
30523691	4	13	used	used	883:886	arg2	studies					857:863	studies	857:863	studies to date that have used MS to identify O-fucosylated TSRs	857:920	Accordingly, studies to date that have used MS to identify O-fucosylated TSRs have required manual interpretation of CID mass spectra even when ETD was also employed.
30523691	2	14	theme	O-linked	495:502	arg1	glycans					504:510	O-linked glycans	495:510	O-linked glycans	495:510	Collision-induced dissociation (CID) spectra of O-fucosylated peptides cannot be sequenced by standard tandem mass spectrometry (MS/MS) sequence database search engines because O-linked glycans are highly labile in the gas phase and are effectively absent from the CID peptide fragment spectra, resulting in a large mass error.
30523691	5	15	theme	automated	1251:1259	arg1	sequencing					1261:1270	automated sequencing	1251:1270	automated sequencing of peptides exhibiting the neutral losses characteristic of labile O-linked glycans	1251:1354	In order to facilitate high-throughput, automatic identification of O-fucosylated peptides from CID spectra, we re-engineered the MS/MS sequence database search engine Comet and the MS data analysis suite Trans-Proteomic Pipeline to enable automated sequencing of peptides exhibiting the neutral losses characteristic of labile O-linked glycans.
30523691	3	16	theme	tryptic	753:759	arg1	peptides					761:768	tryptic peptides	753:768	tryptic peptides with low m/ z	753:782	Electron transfer dissociation (ETD) preserves O-linked glycans on peptide fragments, but only a subset of tryptic peptides with low m/ z can be reliably sequenced from ETD spectra compared to CID.
30523691	6	17	theme	proteomics	1401:1410	arg1	data					1412:1415	published proteomics data	1391:1415	published proteomics data from Plasmodium parasites	1391:1441	We used our approach to reanalyze published proteomics data from Plasmodium parasites and identified multiple glycoforms of TSR-containing proteins.
30523691	4	18	theme	required	927:934	arg1	interpretation					943:956	required manual interpretation	927:956	required manual interpretation of CID mass spectra	927:976	Accordingly, studies to date that have used MS to identify O-fucosylated TSRs have required manual interpretation of CID mass spectra even when ETD was also employed.
30523691	5	19	theme	O-fucosylated	1079:1091	arg1	peptides					1093:1100	O-fucosylated peptides	1079:1100	O-fucosylated peptides from CID spectra	1079:1117	In order to facilitate high-throughput, automatic identification of O-fucosylated peptides from CID spectra, we re-engineered the MS/MS sequence database search engine Comet and the MS data analysis suite Trans-Proteomic Pipeline to enable automated sequencing of peptides exhibiting the neutral losses characteristic of labile O-linked glycans.
30523691	2	20	theme	large	628:632	arg1	error					639:643	a large mass error	626:643	a large mass error	626:643	Collision-induced dissociation (CID) spectra of O-fucosylated peptides cannot be sequenced by standard tandem mass spectrometry (MS/MS) sequence database search engines because O-linked glycans are highly labile in the gas phase and are effectively absent from the CID peptide fragment spectra, resulting in a large mass error.
30523691	5	21	theme	Trans-Proteomic	1216:1230	arg1	Pipeline					1232:1239	suite Trans-Proteomic Pipeline	1210:1239	the MS data analysis suite Trans-Proteomic Pipeline	1189:1239	In order to facilitate high-throughput, automatic identification of O-fucosylated peptides from CID spectra, we re-engineered the MS/MS sequence database search engine Comet and the MS data analysis suite Trans-Proteomic Pipeline to enable automated sequencing of peptides exhibiting the neutral losses characteristic of labile O-linked glycans.
30523691	3	22	theme	m/	779:780	arg1	z					782:782	low m/ z	775:782	low m/ z	775:782	Electron transfer dissociation (ETD) preserves O-linked glycans on peptide fragments, but only a subset of tryptic peptides with low m/ z can be reliably sequenced from ETD spectra compared to CID.
30523691	0	23	theme	Proteins	95:102	arg1	Identification					63:76	Identification	63:76	Identification of O-Fucosylated Proteins	63:102	A Tandem Mass Spectrometry Sequence Database Search Method for Identification of O-Fucosylated Proteins by Mass Spectrometry.
30523691	5	24	theme	neutral	1299:1305	arg1	losses					1307:1312	the neutral losses	1295:1312	the neutral losses characteristic of labile O-linked glycans	1295:1354	In order to facilitate high-throughput, automatic identification of O-fucosylated peptides from CID spectra, we re-engineered the MS/MS sequence database search engine Comet and the MS data analysis suite Trans-Proteomic Pipeline to enable automated sequencing of peptides exhibiting the neutral losses characteristic of labile O-linked glycans.
30523691	0	25	theme	Mass	107:110	arg1	Spectrometry					112:123	Mass Spectrometry	107:123	Mass Spectrometry	107:123	A Tandem Mass Spectrometry Sequence Database Search Method for Identification of O-Fucosylated Proteins by Mass Spectrometry.
30523691	6	26	gly	glycoforms	1467:1476	arg1	proteins					1496:1503	TSR-containing proteins	1481:1503	TSR-containing proteins	1481:1503	We used our approach to reanalyze published proteomics data from Plasmodium parasites and identified multiple glycoforms of TSR-containing proteins.
30523691	6	27	theme	published	1391:1399	arg1	data					1412:1415	published proteomics data	1391:1415	published proteomics data from Plasmodium parasites	1391:1441	We used our approach to reanalyze published proteomics data from Plasmodium parasites and identified multiple glycoforms of TSR-containing proteins.
30523691	4	28	theme	spectra	970:976	arg1	interpretation					943:956	required manual interpretation	927:956	required manual interpretation of CID mass spectra	927:976	Accordingly, studies to date that have used MS to identify O-fucosylated TSRs have required manual interpretation of CID mass spectra even when ETD was also employed.
30523691	5	29	theme	characteristic	1314:1327	arg1	losses					1307:1312	the neutral losses	1295:1312	the neutral losses characteristic of labile O-linked glycans	1295:1354	In order to facilitate high-throughput, automatic identification of O-fucosylated peptides from CID spectra, we re-engineered the MS/MS sequence database search engine Comet and the MS data analysis suite Trans-Proteomic Pipeline to enable automated sequencing of peptides exhibiting the neutral losses characteristic of labile O-linked glycans.
30523691	2	30	theme	O-fucosylated	366:378	arg1	peptides					380:387	O-fucosylated peptides	366:387	O-fucosylated peptides	366:387	Collision-induced dissociation (CID) spectra of O-fucosylated peptides cannot be sequenced by standard tandem mass spectrometry (MS/MS) sequence database search engines because O-linked glycans are highly labile in the gas phase and are effectively absent from the CID peptide fragment spectra, resulting in a large mass error.
30523691	1	31	theme	O-linked	253:260	arg1	fucose					262:267	O-linked fucose	253:267	O-linked fucose	253:267	Thrombospondin type 1 repeats (TSRs), small adhesive protein domains with a wide range of functions, are usually modified with O-linked fucose, which may be extended to O-fucose-β1,3-glucose.
30523691	2	32	theme	mass	428:431	arg1	spectrometry					433:444	tandem mass spectrometry	421:444	standard tandem mass spectrometry (MS/MS) sequence database search engines because O-linked glycans are highly labile in the gas phase and are effectively absent from the CID peptide fragment spectra, resulting in a large mass error	412:643	Collision-induced dissociation (CID) spectra of O-fucosylated peptides cannot be sequenced by standard tandem mass spectrometry (MS/MS) sequence database search engines because O-linked glycans are highly labile in the gas phase and are effectively absent from the CID peptide fragment spectra, resulting in a large mass error.
30523691	2	32	theme	mass	428:431	arg1	MS/MS					447:451	MS/MS	447:451	MS/MS	447:451	Collision-induced dissociation (CID) spectra of O-fucosylated peptides cannot be sequenced by standard tandem mass spectrometry (MS/MS) sequence database search engines because O-linked glycans are highly labile in the gas phase and are effectively absent from the CID peptide fragment spectra, resulting in a large mass error.
30523691	2	33	theme	standard	412:419	arg1	engines					479:485	standard tandem mass spectrometry (MS/MS) sequence database search engines	412:485	standard tandem mass spectrometry (MS/MS) sequence database search engines because O-linked glycans are highly labile in the gas phase and are effectively absent from the CID peptide fragment spectra, resulting in a large mass error	412:643	Collision-induced dissociation (CID) spectra of O-fucosylated peptides cannot be sequenced by standard tandem mass spectrometry (MS/MS) sequence database search engines because O-linked glycans are highly labile in the gas phase and are effectively absent from the CID peptide fragment spectra, resulting in a large mass error.
30523691	0	34	theme	Mass	9:12	arg1	Method					52:57	A Tandem Mass Spectrometry Sequence Database Search Method	0:57	A Tandem Mass Spectrometry Sequence Database Search Method for Identification of O-Fucosylated Proteins by Mass Spectrometry.	0:124	A Tandem Mass Spectrometry Sequence Database Search Method for Identification of O-Fucosylated Proteins by Mass Spectrometry.
30523691	2	35	theme	database	463:470	arg1	engines					479:485	standard tandem mass spectrometry (MS/MS) sequence database search engines	412:485	standard tandem mass spectrometry (MS/MS) sequence database search engines because O-linked glycans are highly labile in the gas phase and are effectively absent from the CID peptide fragment spectra, resulting in a large mass error	412:643	Collision-induced dissociation (CID) spectra of O-fucosylated peptides cannot be sequenced by standard tandem mass spectrometry (MS/MS) sequence database search engines because O-linked glycans are highly labile in the gas phase and are effectively absent from the CID peptide fragment spectra, resulting in a large mass error.
30523691	0	36	theme	Sequence	27:34	arg1	Method					52:57	A Tandem Mass Spectrometry Sequence Database Search Method	0:57	A Tandem Mass Spectrometry Sequence Database Search Method for Identification of O-Fucosylated Proteins by Mass Spectrometry.	0:124	A Tandem Mass Spectrometry Sequence Database Search Method for Identification of O-Fucosylated Proteins by Mass Spectrometry.
30523691	2	37	theme	sequence	454:461	arg1	engines					479:485	standard tandem mass spectrometry (MS/MS) sequence database search engines	412:485	standard tandem mass spectrometry (MS/MS) sequence database search engines because O-linked glycans are highly labile in the gas phase and are effectively absent from the CID peptide fragment spectra, resulting in a large mass error	412:643	Collision-induced dissociation (CID) spectra of O-fucosylated peptides cannot be sequenced by standard tandem mass spectrometry (MS/MS) sequence database search engines because O-linked glycans are highly labile in the gas phase and are effectively absent from the CID peptide fragment spectra, resulting in a large mass error.
30523691	4	38	theme	CID	961:963	arg1	spectra					970:976	CID mass spectra	961:976	CID mass spectra	961:976	Accordingly, studies to date that have used MS to identify O-fucosylated TSRs have required manual interpretation of CID mass spectra even when ETD was also employed.
30523691	2	39	theme	dissociation	336:347	arg1	spectra					355:361	Collision-induced dissociation (CID) spectra	318:361	Collision-induced dissociation (CID) spectra of O-fucosylated peptides	318:387	Collision-induced dissociation (CID) spectra of O-fucosylated peptides cannot be sequenced by standard tandem mass spectrometry (MS/MS) sequence database search engines because O-linked glycans are highly labile in the gas phase and are effectively absent from the CID peptide fragment spectra, resulting in a large mass error.
30523691	0	40	theme	Search	45:50	arg1	Method					52:57	A Tandem Mass Spectrometry Sequence Database Search Method	0:57	A Tandem Mass Spectrometry Sequence Database Search Method for Identification of O-Fucosylated Proteins by Mass Spectrometry.	0:124	A Tandem Mass Spectrometry Sequence Database Search Method for Identification of O-Fucosylated Proteins by Mass Spectrometry.
30523691	5	41	dep	high-throughput	1034:1048	arg1	automatic					1051:1059	automatic	1051:1059	automatic	1051:1059	In order to facilitate high-throughput, automatic identification of O-fucosylated peptides from CID spectra, we re-engineered the MS/MS sequence database search engine Comet and the MS data analysis suite Trans-Proteomic Pipeline to enable automated sequencing of peptides exhibiting the neutral losses characteristic of labile O-linked glycans.
30523691	3	42	theme	transfer	655:662	arg1	ETD					678:680	ETD	678:680	ETD	678:680	Electron transfer dissociation (ETD) preserves O-linked glycans on peptide fragments, but only a subset of tryptic peptides with low m/ z can be reliably sequenced from ETD spectra compared to CID.
30523691	3	42	theme	transfer	655:662	arg1	dissociation					664:675	Electron transfer dissociation	646:675	Electron transfer dissociation (ETD)	646:681	Electron transfer dissociation (ETD) preserves O-linked glycans on peptide fragments, but only a subset of tryptic peptides with low m/ z can be reliably sequenced from ETD spectra compared to CID.
30523691	6	43	theme	TSR-containing	1481:1494	arg1	proteins					1496:1503	TSR-containing proteins	1481:1503	TSR-containing proteins	1481:1503	We used our approach to reanalyze published proteomics data from Plasmodium parasites and identified multiple glycoforms of TSR-containing proteins.
30523691	6	44	from	parasites	1433:1441	arg1	data					1412:1415	published proteomics data	1391:1415	published proteomics data from Plasmodium parasites	1391:1441	We used our approach to reanalyze published proteomics data from Plasmodium parasites and identified multiple glycoforms of TSR-containing proteins.
30523691	4	45	contain	have	922:925	arg1	studies					857:863	studies	857:863	studies to date that have used MS to identify O-fucosylated TSRs	857:920	Accordingly, studies to date that have used MS to identify O-fucosylated TSRs have required manual interpretation of CID mass spectra even when ETD was also employed.
30523691	4	45	contain	have	922:925	arg2	interpretation					943:956	required manual interpretation	927:956	required manual interpretation of CID mass spectra	927:976	Accordingly, studies to date that have used MS to identify O-fucosylated TSRs have required manual interpretation of CID mass spectra even when ETD was also employed.
30523691	5	46	theme	labile	1332:1337	arg1	glycans					1348:1354	labile O-linked glycans	1332:1354	labile O-linked glycans	1332:1354	In order to facilitate high-throughput, automatic identification of O-fucosylated peptides from CID spectra, we re-engineered the MS/MS sequence database search engine Comet and the MS data analysis suite Trans-Proteomic Pipeline to enable automated sequencing of peptides exhibiting the neutral losses characteristic of labile O-linked glycans.
30523691	1	47	theme	functions	216:224	arg1	range					207:211	a wide range	200:211	a wide range of functions	200:224	Thrombospondin type 1 repeats (TSRs), small adhesive protein domains with a wide range of functions, are usually modified with O-linked fucose, which may be extended to O-fucose-β1,3-glucose.
30523691	5	48	from	spectra	1111:1117	arg1	identification					1061:1074	high-throughput, automatic identification	1034:1074	high-throughput, automatic identification of O-fucosylated peptides from CID spectra	1034:1117	In order to facilitate high-throughput, automatic identification of O-fucosylated peptides from CID spectra, we re-engineered the MS/MS sequence database search engine Comet and the MS data analysis suite Trans-Proteomic Pipeline to enable automated sequencing of peptides exhibiting the neutral losses characteristic of labile O-linked glycans.
30523691	5	48	from	spectra	1111:1117	arg1	peptides					1093:1100	O-fucosylated peptides	1079:1100	O-fucosylated peptides from CID spectra	1079:1117	In order to facilitate high-throughput, automatic identification of O-fucosylated peptides from CID spectra, we re-engineered the MS/MS sequence database search engine Comet and the MS data analysis suite Trans-Proteomic Pipeline to enable automated sequencing of peptides exhibiting the neutral losses characteristic of labile O-linked glycans.
30523691	5	49	theme	glycans	1348:1354	arg1	characteristic					1314:1327	characteristic	1314:1327	characteristic	1314:1327	In order to facilitate high-throughput, automatic identification of O-fucosylated peptides from CID spectra, we re-engineered the MS/MS sequence database search engine Comet and the MS data analysis suite Trans-Proteomic Pipeline to enable automated sequencing of peptides exhibiting the neutral losses characteristic of labile O-linked glycans.
30523691	2	50	theme	CID	350:352	arg1	spectra					355:361	Collision-induced dissociation (CID) spectra	318:361	Collision-induced dissociation (CID) spectra of O-fucosylated peptides	318:387	Collision-induced dissociation (CID) spectra of O-fucosylated peptides cannot be sequenced by standard tandem mass spectrometry (MS/MS) sequence database search engines because O-linked glycans are highly labile in the gas phase and are effectively absent from the CID peptide fragment spectra, resulting in a large mass error.
30523691	5	51	theme	sequence	1147:1154	arg1	database					1156:1163	MS/MS sequence database	1141:1163	MS/MS sequence database	1141:1163	In order to facilitate high-throughput, automatic identification of O-fucosylated peptides from CID spectra, we re-engineered the MS/MS sequence database search engine Comet and the MS data analysis suite Trans-Proteomic Pipeline to enable automated sequencing of peptides exhibiting the neutral losses characteristic of labile O-linked glycans.
30523691	2	52	theme	CID	583:585	arg1	spectra					604:610	the CID peptide fragment spectra	579:610	the CID peptide fragment spectra	579:610	Collision-induced dissociation (CID) spectra of O-fucosylated peptides cannot be sequenced by standard tandem mass spectrometry (MS/MS) sequence database search engines because O-linked glycans are highly labile in the gas phase and are effectively absent from the CID peptide fragment spectra, resulting in a large mass error.
30523691	5	53	theme	search	1165:1170	arg1	engine					1172:1177	the MS/MS sequence database search engine Comet and the MS data analysis suite Trans-Proteomic Pipeline	1137:1239	engine	1172:1177	In order to facilitate high-throughput, automatic identification of O-fucosylated peptides from CID spectra, we re-engineered the MS/MS sequence database search engine Comet and the MS data analysis suite Trans-Proteomic Pipeline to enable automated sequencing of peptides exhibiting the neutral losses characteristic of labile O-linked glycans.
30523691	6	54	used	used	1360:1363	arg2	We					1357:1358	We	1357:1358	We	1357:1358	We used our approach to reanalyze published proteomics data from Plasmodium parasites and identified multiple glycoforms of TSR-containing proteins.
30523691	3	55	theme	O-linked	693:700	arg1	glycans					702:708	O-linked glycans	693:708	O-linked glycans on peptide fragments	693:729	Electron transfer dissociation (ETD) preserves O-linked glycans on peptide fragments, but only a subset of tryptic peptides with low m/ z can be reliably sequenced from ETD spectra compared to CID.
30523691	1	56	theme	adhesive	170:177	arg1	repeats					148:154	Thrombospondin type 1 repeats	126:154	Thrombospondin type 1 repeats (TSRs)	126:161	Thrombospondin type 1 repeats (TSRs), small adhesive protein domains with a wide range of functions, are usually modified with O-linked fucose, which may be extended to O-fucose-β1,3-glucose.
30523691	1	56	theme	adhesive	170:177	arg1	domains					187:193	small adhesive protein domains	164:193	small adhesive protein domains with a wide range of functions	164:224	Thrombospondin type 1 repeats (TSRs), small adhesive protein domains with a wide range of functions, are usually modified with O-linked fucose, which may be extended to O-fucose-β1,3-glucose.
30523691	2	57	theme	fragment	595:602	arg1	spectra					604:610	the CID peptide fragment spectra	579:610	the CID peptide fragment spectra	579:610	Collision-induced dissociation (CID) spectra of O-fucosylated peptides cannot be sequenced by standard tandem mass spectrometry (MS/MS) sequence database search engines because O-linked glycans are highly labile in the gas phase and are effectively absent from the CID peptide fragment spectra, resulting in a large mass error.
30523691	3	58	from	glycans	702:708	arg1	fragments					721:729	peptide fragments	713:729	peptide fragments	713:729	Electron transfer dissociation (ETD) preserves O-linked glycans on peptide fragments, but only a subset of tryptic peptides with low m/ z can be reliably sequenced from ETD spectra compared to CID.
30523691	2	59	theme	gas	537:539	arg1	phase					541:545	the gas phase	533:545	the gas phase	533:545	Collision-induced dissociation (CID) spectra of O-fucosylated peptides cannot be sequenced by standard tandem mass spectrometry (MS/MS) sequence database search engines because O-linked glycans are highly labile in the gas phase and are effectively absent from the CID peptide fragment spectra, resulting in a large mass error.
30523691	2	60	link	O-linked	495:502	arg1	glycans					504:510	O-linked glycans	495:510	O-linked glycans	495:510	Collision-induced dissociation (CID) spectra of O-fucosylated peptides cannot be sequenced by standard tandem mass spectrometry (MS/MS) sequence database search engines because O-linked glycans are highly labile in the gas phase and are effectively absent from the CID peptide fragment spectra, resulting in a large mass error.
30523691	1	61	theme	type	141:144	arg1	TSRs					157:160	TSRs	157:160	TSRs	157:160	Thrombospondin type 1 repeats (TSRs), small adhesive protein domains with a wide range of functions, are usually modified with O-linked fucose, which may be extended to O-fucose-β1,3-glucose.
30523691	1	61	theme	type	141:144	arg1	domains					187:193	small adhesive protein domains	164:193	small adhesive protein domains with a wide range of functions	164:224	Thrombospondin type 1 repeats (TSRs), small adhesive protein domains with a wide range of functions, are usually modified with O-linked fucose, which may be extended to O-fucose-β1,3-glucose.
30523691	1	61	theme	type	141:144	arg1	repeats					148:154	Thrombospondin type 1 repeats	126:154	Thrombospondin type 1 repeats (TSRs)	126:161	Thrombospondin type 1 repeats (TSRs), small adhesive protein domains with a wide range of functions, are usually modified with O-linked fucose, which may be extended to O-fucose-β1,3-glucose.
30523691	3	62	theme	ETD	815:817	arg1	spectra					819:825	ETD spectra	815:825	ETD spectra	815:825	Electron transfer dissociation (ETD) preserves O-linked glycans on peptide fragments, but only a subset of tryptic peptides with low m/ z can be reliably sequenced from ETD spectra compared to CID.
30523691	2	63	from	labile	523:528	arg1	phase					541:545	the gas phase	533:545	the gas phase	533:545	Collision-induced dissociation (CID) spectra of O-fucosylated peptides cannot be sequenced by standard tandem mass spectrometry (MS/MS) sequence database search engines because O-linked glycans are highly labile in the gas phase and are effectively absent from the CID peptide fragment spectra, resulting in a large mass error.
30523691	3	64	theme	low	775:777	arg1	z					782:782	low m/ z	775:782	low m/ z	775:782	Electron transfer dissociation (ETD) preserves O-linked glycans on peptide fragments, but only a subset of tryptic peptides with low m/ z can be reliably sequenced from ETD spectra compared to CID.
30523691	3	65	with	peptides	761:768	arg1	z					782:782	low m/ z	775:782	low m/ z	775:782	Electron transfer dissociation (ETD) preserves O-linked glycans on peptide fragments, but only a subset of tryptic peptides with low m/ z can be reliably sequenced from ETD spectra compared to CID.
30523691	4	66	theme	O-fucosylated	903:915	arg1	TSRs					917:920	O-fucosylated TSRs	903:920	O-fucosylated TSRs	903:920	Accordingly, studies to date that have used MS to identify O-fucosylated TSRs have required manual interpretation of CID mass spectra even when ETD was also employed.
30523691	5	67	theme	data	1196:1199	arg1	analysis					1201:1208	the MS data analysis	1189:1208	the MS data analysis suite Trans-Proteomic Pipeline	1189:1239	In order to facilitate high-throughput, automatic identification of O-fucosylated peptides from CID spectra, we re-engineered the MS/MS sequence database search engine Comet and the MS data analysis suite Trans-Proteomic Pipeline to enable automated sequencing of peptides exhibiting the neutral losses characteristic of labile O-linked glycans.
30523691	3	68	theme	peptides	761:768	arg1	subset					743:748	only a subset	736:748	only a subset of tryptic peptides with low m/ z	736:782	Electron transfer dissociation (ETD) preserves O-linked glycans on peptide fragments, but only a subset of tryptic peptides with low m/ z can be reliably sequenced from ETD spectra compared to CID.
30523691	0	69	theme	O-Fucosylated	81:93	arg1	Proteins					95:102	O-Fucosylated Proteins	81:102	O-Fucosylated Proteins	81:102	A Tandem Mass Spectrometry Sequence Database Search Method for Identification of O-Fucosylated Proteins by Mass Spectrometry.
30523691	2	70	theme	search	472:477	arg1	engines					479:485	standard tandem mass spectrometry (MS/MS) sequence database search engines	412:485	standard tandem mass spectrometry (MS/MS) sequence database search engines because O-linked glycans are highly labile in the gas phase and are effectively absent from the CID peptide fragment spectra, resulting in a large mass error	412:643	Collision-induced dissociation (CID) spectra of O-fucosylated peptides cannot be sequenced by standard tandem mass spectrometry (MS/MS) sequence database search engines because O-linked glycans are highly labile in the gas phase and are effectively absent from the CID peptide fragment spectra, resulting in a large mass error.
30523691	2	71	theme	mass	634:637	arg1	error					639:643	a large mass error	626:643	a large mass error	626:643	Collision-induced dissociation (CID) spectra of O-fucosylated peptides cannot be sequenced by standard tandem mass spectrometry (MS/MS) sequence database search engines because O-linked glycans are highly labile in the gas phase and are effectively absent from the CID peptide fragment spectra, resulting in a large mass error.
30523691	5	72	theme	suite	1210:1214	arg1	Pipeline					1232:1239	suite Trans-Proteomic Pipeline	1210:1239	the MS data analysis suite Trans-Proteomic Pipeline	1189:1239	In order to facilitate high-throughput, automatic identification of O-fucosylated peptides from CID spectra, we re-engineered the MS/MS sequence database search engine Comet and the MS data analysis suite Trans-Proteomic Pipeline to enable automated sequencing of peptides exhibiting the neutral losses characteristic of labile O-linked glycans.
30523691	5	73	theme	peptides	1093:1100	arg1	identification					1061:1074	high-throughput, automatic identification	1034:1074	high-throughput, automatic identification of O-fucosylated peptides from CID spectra	1034:1117	In order to facilitate high-throughput, automatic identification of O-fucosylated peptides from CID spectra, we re-engineered the MS/MS sequence database search engine Comet and the MS data analysis suite Trans-Proteomic Pipeline to enable automated sequencing of peptides exhibiting the neutral losses characteristic of labile O-linked glycans.
30523691	4	74	gly	O-fucosylated	903:915	arg1	TSRs					917:920	O-fucosylated TSRs	903:920	O-fucosylated TSRs	903:920	Accordingly, studies to date that have used MS to identify O-fucosylated TSRs have required manual interpretation of CID mass spectra even when ETD was also employed.
30523691	5	75	theme	high-throughput	1034:1048	arg1	identification					1061:1074	high-throughput, automatic identification	1034:1074	high-throughput, automatic identification of O-fucosylated peptides from CID spectra	1034:1117	In order to facilitate high-throughput, automatic identification of O-fucosylated peptides from CID spectra, we re-engineered the MS/MS sequence database search engine Comet and the MS data analysis suite Trans-Proteomic Pipeline to enable automated sequencing of peptides exhibiting the neutral losses characteristic of labile O-linked glycans.
30523691	2	76	theme	peptides	380:387	arg1	spectra					355:361	Collision-induced dissociation (CID) spectra	318:361	Collision-induced dissociation (CID) spectra of O-fucosylated peptides	318:387	Collision-induced dissociation (CID) spectra of O-fucosylated peptides cannot be sequenced by standard tandem mass spectrometry (MS/MS) sequence database search engines because O-linked glycans are highly labile in the gas phase and are effectively absent from the CID peptide fragment spectra, resulting in a large mass error.
30523691	0	77	theme	Tandem	2:7	arg1	Method					52:57	A Tandem Mass Spectrometry Sequence Database Search Method	0:57	A Tandem Mass Spectrometry Sequence Database Search Method for Identification of O-Fucosylated Proteins by Mass Spectrometry.	0:124	A Tandem Mass Spectrometry Sequence Database Search Method for Identification of O-Fucosylated Proteins by Mass Spectrometry.
30523691	2	78	theme	spectrometry	433:444	arg1	engines					479:485	standard tandem mass spectrometry (MS/MS) sequence database search engines	412:485	standard tandem mass spectrometry (MS/MS) sequence database search engines because O-linked glycans are highly labile in the gas phase and are effectively absent from the CID peptide fragment spectra, resulting in a large mass error	412:643	Collision-induced dissociation (CID) spectra of O-fucosylated peptides cannot be sequenced by standard tandem mass spectrometry (MS/MS) sequence database search engines because O-linked glycans are highly labile in the gas phase and are effectively absent from the CID peptide fragment spectra, resulting in a large mass error.
30523691	5	79	link	O-linked	1339:1346	arg1	glycans					1348:1354	labile O-linked glycans	1332:1354	labile O-linked glycans	1332:1354	In order to facilitate high-throughput, automatic identification of O-fucosylated peptides from CID spectra, we re-engineered the MS/MS sequence database search engine Comet and the MS data analysis suite Trans-Proteomic Pipeline to enable automated sequencing of peptides exhibiting the neutral losses characteristic of labile O-linked glycans.
30523691	2	80	gly	O-fucosylated	366:378	arg1	peptides					380:387	O-fucosylated peptides	366:387	O-fucosylated peptides	366:387	Collision-induced dissociation (CID) spectra of O-fucosylated peptides cannot be sequenced by standard tandem mass spectrometry (MS/MS) sequence database search engines because O-linked glycans are highly labile in the gas phase and are effectively absent from the CID peptide fragment spectra, resulting in a large mass error.
30523691	0	81	theme	Spectrometry	14:25	arg1	Method					52:57	A Tandem Mass Spectrometry Sequence Database Search Method	0:57	A Tandem Mass Spectrometry Sequence Database Search Method for Identification of O-Fucosylated Proteins by Mass Spectrometry.	0:124	A Tandem Mass Spectrometry Sequence Database Search Method for Identification of O-Fucosylated Proteins by Mass Spectrometry.
30523691	2	82	theme	tandem	421:426	arg1	spectrometry					433:444	tandem mass spectrometry	421:444	standard tandem mass spectrometry (MS/MS) sequence database search engines because O-linked glycans are highly labile in the gas phase and are effectively absent from the CID peptide fragment spectra, resulting in a large mass error	412:643	Collision-induced dissociation (CID) spectra of O-fucosylated peptides cannot be sequenced by standard tandem mass spectrometry (MS/MS) sequence database search engines because O-linked glycans are highly labile in the gas phase and are effectively absent from the CID peptide fragment spectra, resulting in a large mass error.
30523691	2	82	theme	tandem	421:426	arg1	MS/MS					447:451	MS/MS	447:451	MS/MS	447:451	Collision-induced dissociation (CID) spectra of O-fucosylated peptides cannot be sequenced by standard tandem mass spectrometry (MS/MS) sequence database search engines because O-linked glycans are highly labile in the gas phase and are effectively absent from the CID peptide fragment spectra, resulting in a large mass error.
30523691	5	83	theme	peptides	1275:1282	arg1	sequencing					1261:1270	automated sequencing	1251:1270	automated sequencing of peptides exhibiting the neutral losses characteristic of labile O-linked glycans	1251:1354	In order to facilitate high-throughput, automatic identification of O-fucosylated peptides from CID spectra, we re-engineered the MS/MS sequence database search engine Comet and the MS data analysis suite Trans-Proteomic Pipeline to enable automated sequencing of peptides exhibiting the neutral losses characteristic of labile O-linked glycans.
30523691	0	84	theme	Database	36:43	arg1	Method					52:57	A Tandem Mass Spectrometry Sequence Database Search Method	0:57	A Tandem Mass Spectrometry Sequence Database Search Method for Identification of O-Fucosylated Proteins by Mass Spectrometry.	0:124	A Tandem Mass Spectrometry Sequence Database Search Method for Identification of O-Fucosylated Proteins by Mass Spectrometry.
30523691	5	85	theme	MS/MS	1141:1145	arg1	database					1156:1163	MS/MS sequence database	1141:1163	MS/MS sequence database	1141:1163	In order to facilitate high-throughput, automatic identification of O-fucosylated peptides from CID spectra, we re-engineered the MS/MS sequence database search engine Comet and the MS data analysis suite Trans-Proteomic Pipeline to enable automated sequencing of peptides exhibiting the neutral losses characteristic of labile O-linked glycans.
30523691	1	86	with	domains	187:193	arg1	range					207:211	a wide range	200:211	a wide range of functions	200:224	Thrombospondin type 1 repeats (TSRs), small adhesive protein domains with a wide range of functions, are usually modified with O-linked fucose, which may be extended to O-fucose-β1,3-glucose.
30523691	4	87	theme	manual	936:941	arg1	interpretation					943:956	required manual interpretation	927:956	required manual interpretation of CID mass spectra	927:976	Accordingly, studies to date that have used MS to identify O-fucosylated TSRs have required manual interpretation of CID mass spectra even when ETD was also employed.
30523691	2	88	from	spectra	604:610	arg1	absent					567:572	absent	567:572	absent	567:572	Collision-induced dissociation (CID) spectra of O-fucosylated peptides cannot be sequenced by standard tandem mass spectrometry (MS/MS) sequence database search engines because O-linked glycans are highly labile in the gas phase and are effectively absent from the CID peptide fragment spectra, resulting in a large mass error.
30523691	5	89	from	identification	1061:1074	arg1	spectra					1111:1117	CID spectra	1107:1117	CID spectra	1107:1117	In order to facilitate high-throughput, automatic identification of O-fucosylated peptides from CID spectra, we re-engineered the MS/MS sequence database search engine Comet and the MS data analysis suite Trans-Proteomic Pipeline to enable automated sequencing of peptides exhibiting the neutral losses characteristic of labile O-linked glycans.
30523691	5	90	theme	CID	1107:1109	arg1	spectra					1111:1117	CID spectra	1107:1117	CID spectra	1107:1117	In order to facilitate high-throughput, automatic identification of O-fucosylated peptides from CID spectra, we re-engineered the MS/MS sequence database search engine Comet and the MS data analysis suite Trans-Proteomic Pipeline to enable automated sequencing of peptides exhibiting the neutral losses characteristic of labile O-linked glycans.
30523691	3	91	theme	Electron	646:653	arg1	ETD					678:680	ETD	678:680	ETD	678:680	Electron transfer dissociation (ETD) preserves O-linked glycans on peptide fragments, but only a subset of tryptic peptides with low m/ z can be reliably sequenced from ETD spectra compared to CID.
30523691	3	91	theme	Electron	646:653	arg1	dissociation					664:675	Electron transfer dissociation	646:675	Electron transfer dissociation (ETD)	646:681	Electron transfer dissociation (ETD) preserves O-linked glycans on peptide fragments, but only a subset of tryptic peptides with low m/ z can be reliably sequenced from ETD spectra compared to CID.
30523691	2	92	theme	Collision-induced	318:334	arg1	spectra					355:361	Collision-induced dissociation (CID) spectra	318:361	Collision-induced dissociation (CID) spectra of O-fucosylated peptides	318:387	Collision-induced dissociation (CID) spectra of O-fucosylated peptides cannot be sequenced by standard tandem mass spectrometry (MS/MS) sequence database search engines because O-linked glycans are highly labile in the gas phase and are effectively absent from the CID peptide fragment spectra, resulting in a large mass error.
30523691	6	93	theme	proteins	1496:1503	arg1	glycoforms					1467:1476	multiple glycoforms	1458:1476	multiple glycoforms of TSR-containing proteins	1458:1503	We used our approach to reanalyze published proteomics data from Plasmodium parasites and identified multiple glycoforms of TSR-containing proteins.
30523691	1	94	link	O-linked	253:260	arg1	fucose					262:267	O-linked fucose	253:267	O-linked fucose	253:267	Thrombospondin type 1 repeats (TSRs), small adhesive protein domains with a wide range of functions, are usually modified with O-linked fucose, which may be extended to O-fucose-β1,3-glucose.
30523691	2	95	from	phase	541:545	arg1	labile					523:528	labile	523:528	labile	523:528	Collision-induced dissociation (CID) spectra of O-fucosylated peptides cannot be sequenced by standard tandem mass spectrometry (MS/MS) sequence database search engines because O-linked glycans are highly labile in the gas phase and are effectively absent from the CID peptide fragment spectra, resulting in a large mass error.
30523691	5	96	theme	O-linked	1339:1346	arg1	glycans					1348:1354	labile O-linked glycans	1332:1354	labile O-linked glycans	1332:1354	In order to facilitate high-throughput, automatic identification of O-fucosylated peptides from CID spectra, we re-engineered the MS/MS sequence database search engine Comet and the MS data analysis suite Trans-Proteomic Pipeline to enable automated sequencing of peptides exhibiting the neutral losses characteristic of labile O-linked glycans.
30523691	6	97	theme	Plasmodium	1422:1431	arg1	parasites					1433:1441	Plasmodium parasites	1422:1441	Plasmodium parasites	1422:1441	We used our approach to reanalyze published proteomics data from Plasmodium parasites and identified multiple glycoforms of TSR-containing proteins.
30523691	6	98	theme	multiple	1458:1465	arg1	glycoforms					1467:1476	multiple glycoforms	1458:1476	multiple glycoforms of TSR-containing proteins	1458:1503	We used our approach to reanalyze published proteomics data from Plasmodium parasites and identified multiple glycoforms of TSR-containing proteins.
30523691	5	99	theme	MS	1193:1194	arg1	analysis					1201:1208	the MS data analysis	1189:1208	the MS data analysis suite Trans-Proteomic Pipeline	1189:1239	In order to facilitate high-throughput, automatic identification of O-fucosylated peptides from CID spectra, we re-engineered the MS/MS sequence database search engine Comet and the MS data analysis suite Trans-Proteomic Pipeline to enable automated sequencing of peptides exhibiting the neutral losses characteristic of labile O-linked glycans.
31220511	0	0	link	N-linked	83:90	arg1	sites					106:110	N-linked glycosylation sites	83:110	N-linked glycosylation sites	83:110	Conservation region finding for influenza A viruses by machine learning methods of N-linked glycosylation sites and B-cell epitopes.
31220511	8	1	theme	analytic	1040:1047	arg1	tools					1049:1053	available analytic tools	1030:1053	available analytic tools	1030:1053	Using available analytic tools, we searched for the representative strain of each virus subtype.
31220511	3	2	theme	A	476:476	arg1	virus					478:482	the influenza A virus	462:482	the influenza A virus	462:482	The primary target identity regions for the human immune system are hemagglutinin (HA) and neuraminidase (NA), two surface proteins of the influenza A virus.
31220511	1	3	theme	health	246:251	arg1	worldwide					253:261	human health worldwide	240:261	human health worldwide	240:261	Influenza type A, a serious infectious disease of the human respiratory tract, poses an enormous threat to human health worldwide.
31220511	3	4	theme	immune	377:382	arg1	system					384:389	the human immune system	367:389	the human immune system	367:389	The primary target identity regions for the human immune system are hemagglutinin (HA) and neuraminidase (NA), two surface proteins of the influenza A virus.
31220511	8	5	theme	subtype	1112:1118	arg1	strain					1091:1096	the representative strain	1072:1096	the representative strain of each virus subtype	1072:1118	Using available analytic tools, we searched for the representative strain of each virus subtype.
31220511	10	6	theme	Immune	1309:1314	arg1	response					1364:1371	the Immune Epitope Database (IEDB) antibody neutralization response	1305:1371	the Immune Epitope Database (IEDB) antibody neutralization response (i.e., screening of antibody sequence regions)	1305:1418	Compared to the prediction of the Immune Epitope Database (IEDB) antibody neutralization response (i.e., screening of antibody sequence regions), in this study, the virus sequence coverage was large and accurate and contained N-linked glycosylation sites.
31220511	1	7	theme	infectious	161:170	arg1	disease					172:178	a serious infectious disease	151:178	a serious infectious disease of the human respiratory tract	151:209	Influenza type A, a serious infectious disease of the human respiratory tract, poses an enormous threat to human health worldwide.
31220511	1	7	theme	infectious	161:170	arg1	A					148:148	Influenza type A	133:148	Influenza type A	133:148	Influenza type A, a serious infectious disease of the human respiratory tract, poses an enormous threat to human health worldwide.
31220511	2	8	theme	mortality	281:289	arg1	rates					291:295	high mortality rates	276:295	high mortality rates in poultry, pigs, and humans	276:324	It leads to high mortality rates in poultry, pigs, and humans.
31220511	12	9	theme	universal	1827:1835	arg1	design					1855:1860	a universal influenza vaccine design	1825:1860	a universal influenza vaccine design reference in the future	1825:1884	In addition, the screening fragments can be used as a universal influenza vaccine design reference in the future.
31220511	4	10	theme	A	562:562	arg1	virus					564:568	the influenza A virus	548:568	the influenza A virus	548:568	Research and development of vaccines is highly complex because the influenza A virus evolves rapidly.
31220511	10	11	theme	sequence	1446:1453	arg1	large					1468:1472	large	1468:1472	large	1468:1472	Compared to the prediction of the Immune Epitope Database (IEDB) antibody neutralization response (i.e., screening of antibody sequence regions), in this study, the virus sequence coverage was large and accurate and contained N-linked glycosylation sites.
31220511	10	11	theme	sequence	1446:1453	arg1	coverage					1455:1462	the virus sequence coverage	1436:1462	the virus sequence coverage	1436:1462	Compared to the prediction of the Immune Epitope Database (IEDB) antibody neutralization response (i.e., screening of antibody sequence regions), in this study, the virus sequence coverage was large and accurate and contained N-linked glycosylation sites.
31220511	11	12	theme	vaccine	1649:1655	arg1	invalidation					1657:1668	vaccine invalidation	1649:1668	vaccine invalidation that occurred during the rapid evolution of the influenza A virus and also as a prevaccine assessment	1649:1770	The results of this study proved that we can use the machine learning-based prediction method to solve the problem of vaccine invalidation that occurred during the rapid evolution of the influenza A virus and also as a prevaccine assessment.
31220511	0	13	theme	B-cell	116:121	arg1	epitopes					123:130	B-cell epitopes	116:130	B-cell epitopes	116:130	Conservation region finding for influenza A viruses by machine learning methods of N-linked glycosylation sites and B-cell epitopes.
31220511	1	14	theme	human	187:191	arg1	tract					205:209	the human respiratory tract	183:209	the human respiratory tract	183:209	Influenza type A, a serious infectious disease of the human respiratory tract, poses an enormous threat to human health worldwide.
31220511	11	15	theme	prevaccine	1750:1759	arg1	assessment					1761:1770	a prevaccine assessment	1748:1770	a prevaccine assessment	1748:1770	The results of this study proved that we can use the machine learning-based prediction method to solve the problem of vaccine invalidation that occurred during the rapid evolution of the influenza A virus and also as a prevaccine assessment.
31220511	11	16	theme	rapid	1695:1699	arg1	evolution					1701:1709	the rapid evolution	1691:1709	the rapid evolution of the influenza A virus	1691:1734	The results of this study proved that we can use the machine learning-based prediction method to solve the problem of vaccine invalidation that occurred during the rapid evolution of the influenza A virus and also as a prevaccine assessment.
31220511	5	17	theme	surface	641:647	arg1	proteins					649:656	viral surface proteins	635:656	viral surface proteins	635:656	This study focused on three genetic features of viral surface proteins: ribonucleic acid (RNA) sequence conservation, linear B-cell epitopes, and N-linked glycosylation.
31220511	1	18	theme	tract	205:209	arg1	disease					172:178	a serious infectious disease	151:178	a serious infectious disease of the human respiratory tract	151:209	Influenza type A, a serious infectious disease of the human respiratory tract, poses an enormous threat to human health worldwide.
31220511	1	18	theme	tract	205:209	arg1	A					148:148	Influenza type A	133:148	Influenza type A	133:148	Influenza type A, a serious infectious disease of the human respiratory tract, poses an enormous threat to human health worldwide.
31220511	12	19	theme	vaccine	1847:1853	arg1	design					1855:1860	a universal influenza vaccine design	1825:1860	a universal influenza vaccine design reference in the future	1825:1884	In addition, the screening fragments can be used as a universal influenza vaccine design reference in the future.
31220511	6	20	theme	NA	828:829	arg1	sequences					839:847	12,832 HA and 9487 NA protein sequences	809:847	sequences	839:847	On the basis of these three properties, we analyzed 12,832 HA and 9487 NA protein sequences, which we retrieved from the influenza virus database.
31220511	7	21	theme	surface	928:934	arg1	sequences					944:952	the viral surface protein sequences	918:952	the viral surface protein sequences	918:952	We classified the viral surface protein sequences into the 18 HA and 11 NA subtypes that have been identified thus far.
31220511	9	22	theme	machine	1140:1146	arg1	methods					1157:1163	machine learning methods	1140:1163	machine learning methods	1140:1163	Furthermore, using machine learning methods, we looked for conservation regions with sequences showing linear B-cell epitopes and N-linked glycosylation.
31220511	5	23	link	N-linked	733:740	arg1	glycosylation					742:754	N-linked glycosylation	733:754	N-linked glycosylation	733:754	This study focused on three genetic features of viral surface proteins: ribonucleic acid (RNA) sequence conservation, linear B-cell epitopes, and N-linked glycosylation.
31220511	1	24	theme	Influenza	133:141	arg1	disease					172:178	a serious infectious disease	151:178	a serious infectious disease of the human respiratory tract	151:209	Influenza type A, a serious infectious disease of the human respiratory tract, poses an enormous threat to human health worldwide.
31220511	1	24	theme	Influenza	133:141	arg1	A					148:148	Influenza type A	133:148	Influenza type A	133:148	Influenza type A, a serious infectious disease of the human respiratory tract, poses an enormous threat to human health worldwide.
31220511	11	25	theme	learning-based	1592:1605	arg1	method					1618:1623	the machine learning-based prediction method	1580:1623	the machine learning-based prediction method	1580:1623	The results of this study proved that we can use the machine learning-based prediction method to solve the problem of vaccine invalidation that occurred during the rapid evolution of the influenza A virus and also as a prevaccine assessment.
31220511	5	26	theme	RNA	677:679	arg1	conservation					691:702	ribonucleic acid (RNA) sequence conservation	659:702	ribonucleic acid (RNA) sequence conservation	659:702	This study focused on three genetic features of viral surface proteins: ribonucleic acid (RNA) sequence conservation, linear B-cell epitopes, and N-linked glycosylation.
31220511	12	27	theme	influenza	1837:1845	arg1	design					1855:1860	a universal influenza vaccine design	1825:1860	a universal influenza vaccine design reference in the future	1825:1884	In addition, the screening fragments can be used as a universal influenza vaccine design reference in the future.
31220511	10	28	theme	IEDB	1334:1337	arg1	response					1364:1371	the Immune Epitope Database (IEDB) antibody neutralization response	1305:1371	the Immune Epitope Database (IEDB) antibody neutralization response (i.e., screening of antibody sequence regions)	1305:1418	Compared to the prediction of the Immune Epitope Database (IEDB) antibody neutralization response (i.e., screening of antibody sequence regions), in this study, the virus sequence coverage was large and accurate and contained N-linked glycosylation sites.
31220511	5	29	theme	genetic	615:621	arg1	features					623:630	three genetic features	609:630	three genetic features of viral surface proteins	609:656	This study focused on three genetic features of viral surface proteins: ribonucleic acid (RNA) sequence conservation, linear B-cell epitopes, and N-linked glycosylation.
31220511	5	30	theme	sequence	682:689	arg1	conservation					691:702	ribonucleic acid (RNA) sequence conservation	659:702	ribonucleic acid (RNA) sequence conservation	659:702	This study focused on three genetic features of viral surface proteins: ribonucleic acid (RNA) sequence conservation, linear B-cell epitopes, and N-linked glycosylation.
31220511	2	31	from	rates	291:295	arg1	pigs					309:312	pigs	309:312	pigs	309:312	It leads to high mortality rates in poultry, pigs, and humans.
31220511	2	31	from	rates	291:295	arg1	humans					319:324	humans	319:324	humans	319:324	It leads to high mortality rates in poultry, pigs, and humans.
31220511	2	31	from	rates	291:295	arg1	poultry					300:306	poultry	300:306	poultry	300:306	It leads to high mortality rates in poultry, pigs, and humans.
31220511	6	32	theme	virus	888:892	arg1	database					894:901	the influenza virus database	874:901	the influenza virus database	874:901	On the basis of these three properties, we analyzed 12,832 HA and 9487 NA protein sequences, which we retrieved from the influenza virus database.
31220511	10	33	theme	Database	1324:1331	arg1	response					1364:1371	the Immune Epitope Database (IEDB) antibody neutralization response	1305:1371	the Immune Epitope Database (IEDB) antibody neutralization response (i.e., screening of antibody sequence regions)	1305:1418	Compared to the prediction of the Immune Epitope Database (IEDB) antibody neutralization response (i.e., screening of antibody sequence regions), in this study, the virus sequence coverage was large and accurate and contained N-linked glycosylation sites.
31220511	0	34	theme	N-linked	83:90	arg1	sites					106:110	N-linked glycosylation sites	83:110	N-linked glycosylation sites	83:110	Conservation region finding for influenza A viruses by machine learning methods of N-linked glycosylation sites and B-cell epitopes.
31220511	10	35	theme	response	1364:1371	arg1	prediction					1291:1300	the prediction	1287:1300	the prediction of the Immune Epitope Database (IEDB) antibody neutralization response (i.e., screening of antibody sequence regions)	1287:1418	Compared to the prediction of the Immune Epitope Database (IEDB) antibody neutralization response (i.e., screening of antibody sequence regions), in this study, the virus sequence coverage was large and accurate and contained N-linked glycosylation sites.
31220511	5	36	theme	acid	671:674	arg1	conservation					691:702	ribonucleic acid (RNA) sequence conservation	659:702	ribonucleic acid (RNA) sequence conservation	659:702	This study focused on three genetic features of viral surface proteins: ribonucleic acid (RNA) sequence conservation, linear B-cell epitopes, and N-linked glycosylation.
31220511	3	37	theme	target	339:344	arg1	hemagglutinin					395:407	hemagglutinin	395:407	hemagglutinin (HA)	395:412	The primary target identity regions for the human immune system are hemagglutinin (HA) and neuraminidase (NA), two surface proteins of the influenza A virus.
31220511	3	37	theme	target	339:344	arg1	regions					355:361	The primary target identity regions	327:361	The primary target identity regions for the human immune system	327:389	The primary target identity regions for the human immune system are hemagglutinin (HA) and neuraminidase (NA), two surface proteins of the influenza A virus.
31220511	10	38	theme	antibody	1340:1347	arg1	response					1364:1371	the Immune Epitope Database (IEDB) antibody neutralization response	1305:1371	the Immune Epitope Database (IEDB) antibody neutralization response (i.e., screening of antibody sequence regions)	1305:1418	Compared to the prediction of the Immune Epitope Database (IEDB) antibody neutralization response (i.e., screening of antibody sequence regions), in this study, the virus sequence coverage was large and accurate and contained N-linked glycosylation sites.
31220511	10	39	theme	glycosylation	1510:1522	arg1	sites					1524:1528	N-linked glycosylation sites	1501:1528	N-linked glycosylation sites	1501:1528	Compared to the prediction of the Immune Epitope Database (IEDB) antibody neutralization response (i.e., screening of antibody sequence regions), in this study, the virus sequence coverage was large and accurate and contained N-linked glycosylation sites.
31220511	0	40	theme	Conservation	0:11	arg1	region					13:18	Conservation region	0:18	Conservation region	0:18	Conservation region finding for influenza A viruses by machine learning methods of N-linked glycosylation sites and B-cell epitopes.
31220511	9	41	theme	learning	1148:1155	arg1	methods					1157:1163	machine learning methods	1140:1163	machine learning methods	1140:1163	Furthermore, using machine learning methods, we looked for conservation regions with sequences showing linear B-cell epitopes and N-linked glycosylation.
31220511	0	42	gly	glycosylation	92:104	arg2	sites					106:110	N-linked glycosylation sites	83:110	N-linked glycosylation sites	83:110	Conservation region finding for influenza A viruses by machine learning methods of N-linked glycosylation sites and B-cell epitopes.
31220511	5	43	theme	N-linked	733:740	arg1	glycosylation					742:754	N-linked glycosylation	733:754	N-linked glycosylation	733:754	This study focused on three genetic features of viral surface proteins: ribonucleic acid (RNA) sequence conservation, linear B-cell epitopes, and N-linked glycosylation.
31220511	9	44	theme	linear	1224:1229	arg1	epitopes					1238:1245	linear B-cell epitopes	1224:1245	linear B-cell epitopes	1224:1245	Furthermore, using machine learning methods, we looked for conservation regions with sequences showing linear B-cell epitopes and N-linked glycosylation.
31220511	10	45	link	N-linked	1501:1508	arg1	sites					1524:1528	N-linked glycosylation sites	1501:1528	N-linked glycosylation sites	1501:1528	Compared to the prediction of the Immune Epitope Database (IEDB) antibody neutralization response (i.e., screening of antibody sequence regions), in this study, the virus sequence coverage was large and accurate and contained N-linked glycosylation sites.
31220511	0	46	theme	influenza	32:40	arg1	viruses					44:50	influenza A viruses	32:50	influenza A viruses	32:50	Conservation region finding for influenza A viruses by machine learning methods of N-linked glycosylation sites and B-cell epitopes.
31220511	9	47	link	N-linked	1251:1258	arg1	glycosylation					1260:1272	N-linked glycosylation	1251:1272	N-linked glycosylation	1251:1272	Furthermore, using machine learning methods, we looked for conservation regions with sequences showing linear B-cell epitopes and N-linked glycosylation.
31220511	10	48	theme	regions	1411:1417	arg1	screening					1380:1388	screening	1380:1388	screening of antibody sequence regions	1380:1417	Compared to the prediction of the Immune Epitope Database (IEDB) antibody neutralization response (i.e., screening of antibody sequence regions), in this study, the virus sequence coverage was large and accurate and contained N-linked glycosylation sites.
31220511	0	49	theme	machine	55:61	arg1	methods					72:78	machine learning methods	55:78	machine learning methods of N-linked glycosylation sites and B-cell epitopes	55:130	Conservation region finding for influenza A viruses by machine learning methods of N-linked glycosylation sites and B-cell epitopes.
31220511	11	50	theme	influenza	1718:1726	arg1	virus					1730:1734	the influenza A virus	1714:1734	the influenza A virus	1714:1734	The results of this study proved that we can use the machine learning-based prediction method to solve the problem of vaccine invalidation that occurred during the rapid evolution of the influenza A virus and also as a prevaccine assessment.
31220511	5	51	theme	B-cell	712:717	arg1	epitopes					719:726	linear B-cell epitopes	705:726	linear B-cell epitopes	705:726	This study focused on three genetic features of viral surface proteins: ribonucleic acid (RNA) sequence conservation, linear B-cell epitopes, and N-linked glycosylation.
31220511	9	52	theme	N-linked	1251:1258	arg1	glycosylation					1260:1272	N-linked glycosylation	1251:1272	N-linked glycosylation	1251:1272	Furthermore, using machine learning methods, we looked for conservation regions with sequences showing linear B-cell epitopes and N-linked glycosylation.
31220511	10	53	theme	antibody	1393:1400	arg1	regions					1411:1417	antibody sequence regions	1393:1417	antibody sequence regions	1393:1417	Compared to the prediction of the Immune Epitope Database (IEDB) antibody neutralization response (i.e., screening of antibody sequence regions), in this study, the virus sequence coverage was large and accurate and contained N-linked glycosylation sites.
31220511	11	54	theme	machine	1584:1590	arg1	method					1618:1623	the machine learning-based prediction method	1580:1623	the machine learning-based prediction method	1580:1623	The results of this study proved that we can use the machine learning-based prediction method to solve the problem of vaccine invalidation that occurred during the rapid evolution of the influenza A virus and also as a prevaccine assessment.
31220511	6	55	dep	properties	785:794	arg1	the					760:762	the	760:762	the	760:762	On the basis of these three properties, we analyzed 12,832 HA and 9487 NA protein sequences, which we retrieved from the influenza virus database.
31220511	6	55	dep	properties	785:794	arg1	basis					764:768	basis	764:768	basis	764:768	On the basis of these three properties, we analyzed 12,832 HA and 9487 NA protein sequences, which we retrieved from the influenza virus database.
31220511	3	56	theme	surface	442:448	arg1	proteins					450:457	two surface proteins	438:457	two surface proteins of the influenza A virus	438:482	The primary target identity regions for the human immune system are hemagglutinin (HA) and neuraminidase (NA), two surface proteins of the influenza A virus.
31220511	10	57	contain	contained	1491:1499	arg1	large					1468:1472	large	1468:1472	large	1468:1472	Compared to the prediction of the Immune Epitope Database (IEDB) antibody neutralization response (i.e., screening of antibody sequence regions), in this study, the virus sequence coverage was large and accurate and contained N-linked glycosylation sites.
31220511	10	57	contain	contained	1491:1499	arg1	coverage					1455:1462	the virus sequence coverage	1436:1462	the virus sequence coverage	1436:1462	Compared to the prediction of the Immune Epitope Database (IEDB) antibody neutralization response (i.e., screening of antibody sequence regions), in this study, the virus sequence coverage was large and accurate and contained N-linked glycosylation sites.
31220511	10	57	contain	contained	1491:1499	arg2	sites					1524:1528	N-linked glycosylation sites	1501:1528	N-linked glycosylation sites	1501:1528	Compared to the prediction of the Immune Epitope Database (IEDB) antibody neutralization response (i.e., screening of antibody sequence regions), in this study, the virus sequence coverage was large and accurate and contained N-linked glycosylation sites.
31220511	11	58	theme	virus	1730:1734	arg1	evolution					1701:1709	the rapid evolution	1691:1709	the rapid evolution of the influenza A virus	1691:1734	The results of this study proved that we can use the machine learning-based prediction method to solve the problem of vaccine invalidation that occurred during the rapid evolution of the influenza A virus and also as a prevaccine assessment.
31220511	10	59	from	large	1468:1472	arg1	study					1429:1433	this study	1424:1433	this study	1424:1433	Compared to the prediction of the Immune Epitope Database (IEDB) antibody neutralization response (i.e., screening of antibody sequence regions), in this study, the virus sequence coverage was large and accurate and contained N-linked glycosylation sites.
31220511	10	60	theme	virus	1440:1444	arg1	large					1468:1472	large	1468:1472	large	1468:1472	Compared to the prediction of the Immune Epitope Database (IEDB) antibody neutralization response (i.e., screening of antibody sequence regions), in this study, the virus sequence coverage was large and accurate and contained N-linked glycosylation sites.
31220511	10	60	theme	virus	1440:1444	arg1	coverage					1455:1462	the virus sequence coverage	1436:1462	the virus sequence coverage	1436:1462	Compared to the prediction of the Immune Epitope Database (IEDB) antibody neutralization response (i.e., screening of antibody sequence regions), in this study, the virus sequence coverage was large and accurate and contained N-linked glycosylation sites.
31220511	3	61	theme	influenza	466:474	arg1	virus					478:482	the influenza A virus	462:482	the influenza A virus	462:482	The primary target identity regions for the human immune system are hemagglutinin (HA) and neuraminidase (NA), two surface proteins of the influenza A virus.
31220511	1	62	theme	human	240:244	arg1	worldwide					253:261	human health worldwide	240:261	human health worldwide	240:261	Influenza type A, a serious infectious disease of the human respiratory tract, poses an enormous threat to human health worldwide.
31220511	8	63	theme	available	1030:1038	arg1	tools					1049:1053	available analytic tools	1030:1053	available analytic tools	1030:1053	Using available analytic tools, we searched for the representative strain of each virus subtype.
31220511	12	64	from	reference	1862:1870	arg1	future					1879:1884	future	1879:1884	future	1879:1884	In addition, the screening fragments can be used as a universal influenza vaccine design reference in the future.
31220511	4	65	theme	vaccines	513:520	arg1	development					498:508	development	498:508	development	498:508	Research and development of vaccines is highly complex because the influenza A virus evolves rapidly.
31220511	4	65	theme	vaccines	513:520	arg1	Research					485:492	Research	485:492	Research	485:492	Research and development of vaccines is highly complex because the influenza A virus evolves rapidly.
31220511	7	66	theme	NA	976:977	arg1	subtypes					979:986	the 18 HA and 11 NA subtypes	959:986	subtypes	979:986	We classified the viral surface protein sequences into the 18 HA and 11 NA subtypes that have been identified thus far.
31220511	1	67	theme	serious	153:159	arg1	disease					172:178	a serious infectious disease	151:178	a serious infectious disease of the human respiratory tract	151:209	Influenza type A, a serious infectious disease of the human respiratory tract, poses an enormous threat to human health worldwide.
31220511	1	67	theme	serious	153:159	arg1	A					148:148	Influenza type A	133:148	Influenza type A	133:148	Influenza type A, a serious infectious disease of the human respiratory tract, poses an enormous threat to human health worldwide.
31220511	12	68	theme	design	1855:1860	arg1	reference					1862:1870	a universal influenza vaccine design reference	1825:1870	a universal influenza vaccine design reference in the future	1825:1884	In addition, the screening fragments can be used as a universal influenza vaccine design reference in the future.
31220511	12	68	theme	design	1855:1860	arg1	fragments					1800:1808	the screening fragments	1786:1808	the screening fragments	1786:1808	In addition, the screening fragments can be used as a universal influenza vaccine design reference in the future.
31220511	5	69	gly	glycosylation	742:754	arg1	proteins					649:656	viral surface proteins	635:656	viral surface proteins	635:656	This study focused on three genetic features of viral surface proteins: ribonucleic acid (RNA) sequence conservation, linear B-cell epitopes, and N-linked glycosylation.
31220511	8	70	theme	virus	1106:1110	arg1	subtype					1112:1118	each virus subtype	1101:1118	each virus subtype	1101:1118	Using available analytic tools, we searched for the representative strain of each virus subtype.
31220511	0	71	theme	epitopes	123:130	arg1	methods					72:78	machine learning methods	55:78	machine learning methods of N-linked glycosylation sites and B-cell epitopes	55:130	Conservation region finding for influenza A viruses by machine learning methods of N-linked glycosylation sites and B-cell epitopes.
31220511	2	72	theme	high	276:279	arg1	rates					291:295	high mortality rates	276:295	high mortality rates in poultry, pigs, and humans	276:324	It leads to high mortality rates in poultry, pigs, and humans.
31220511	4	73	theme	influenza	552:560	arg1	virus					564:568	the influenza A virus	548:568	the influenza A virus	548:568	Research and development of vaccines is highly complex because the influenza A virus evolves rapidly.
31220511	11	74	theme	invalidation	1657:1668	arg1	problem					1638:1644	the problem	1634:1644	the problem of vaccine invalidation that occurred during the rapid evolution of the influenza A virus and also as a prevaccine assessment	1634:1770	The results of this study proved that we can use the machine learning-based prediction method to solve the problem of vaccine invalidation that occurred during the rapid evolution of the influenza A virus and also as a prevaccine assessment.
31220511	5	75	theme	viral	635:639	arg1	proteins					649:656	viral surface proteins	635:656	viral surface proteins	635:656	This study focused on three genetic features of viral surface proteins: ribonucleic acid (RNA) sequence conservation, linear B-cell epitopes, and N-linked glycosylation.
31220511	1	76	theme	respiratory	193:203	arg1	tract					205:209	the human respiratory tract	183:209	the human respiratory tract	183:209	Influenza type A, a serious infectious disease of the human respiratory tract, poses an enormous threat to human health worldwide.
31220511	8	77	theme	representative	1076:1089	arg1	strain					1091:1096	the representative strain	1072:1096	the representative strain of each virus subtype	1072:1118	Using available analytic tools, we searched for the representative strain of each virus subtype.
31220511	5	78	theme	proteins	649:656	arg1	conservation					691:702	ribonucleic acid (RNA) sequence conservation	659:702	ribonucleic acid (RNA) sequence conservation	659:702	This study focused on three genetic features of viral surface proteins: ribonucleic acid (RNA) sequence conservation, linear B-cell epitopes, and N-linked glycosylation.
31220511	5	78	theme	proteins	649:656	arg1	glycosylation					742:754	N-linked glycosylation	733:754	N-linked glycosylation	733:754	This study focused on three genetic features of viral surface proteins: ribonucleic acid (RNA) sequence conservation, linear B-cell epitopes, and N-linked glycosylation.
31220511	5	78	theme	proteins	649:656	arg1	epitopes					719:726	linear B-cell epitopes	705:726	linear B-cell epitopes	705:726	This study focused on three genetic features of viral surface proteins: ribonucleic acid (RNA) sequence conservation, linear B-cell epitopes, and N-linked glycosylation.
31220511	5	78	theme	proteins	649:656	arg1	features					623:630	three genetic features	609:630	three genetic features of viral surface proteins	609:656	This study focused on three genetic features of viral surface proteins: ribonucleic acid (RNA) sequence conservation, linear B-cell epitopes, and N-linked glycosylation.
31220511	7	79	theme	protein	936:942	arg1	sequences					944:952	the viral surface protein sequences	918:952	the viral surface protein sequences	918:952	We classified the viral surface protein sequences into the 18 HA and 11 NA subtypes that have been identified thus far.
31220511	6	80	theme	protein	831:837	arg1	sequences					839:847	12,832 HA and 9487 NA protein sequences	809:847	sequences	839:847	On the basis of these three properties, we analyzed 12,832 HA and 9487 NA protein sequences, which we retrieved from the influenza virus database.
31220511	9	81	with	regions	1193:1199	arg1	sequences					1206:1214	sequences	1206:1214	sequences showing linear B-cell epitopes and N-linked glycosylation	1206:1272	Furthermore, using machine learning methods, we looked for conservation regions with sequences showing linear B-cell epitopes and N-linked glycosylation.
31220511	7	82	theme	viral	922:926	arg1	sequences					944:952	the viral surface protein sequences	918:952	the viral surface protein sequences	918:952	We classified the viral surface protein sequences into the 18 HA and 11 NA subtypes that have been identified thus far.
31220511	3	83	theme	virus	478:482	arg1	proteins					450:457	two surface proteins	438:457	two surface proteins of the influenza A virus	438:482	The primary target identity regions for the human immune system are hemagglutinin (HA) and neuraminidase (NA), two surface proteins of the influenza A virus.
31220511	3	83	theme	virus	478:482	arg1	neuraminidase					418:430	neuraminidase	418:430	neuraminidase (NA)	418:435	The primary target identity regions for the human immune system are hemagglutinin (HA) and neuraminidase (NA), two surface proteins of the influenza A virus.
31220511	3	83	theme	virus	478:482	arg1	hemagglutinin					395:407	hemagglutinin	395:407	hemagglutinin (HA)	395:412	The primary target identity regions for the human immune system are hemagglutinin (HA) and neuraminidase (NA), two surface proteins of the influenza A virus.
31220511	3	83	theme	virus	478:482	arg1	regions					355:361	The primary target identity regions	327:361	The primary target identity regions for the human immune system	327:389	The primary target identity regions for the human immune system are hemagglutinin (HA) and neuraminidase (NA), two surface proteins of the influenza A virus.
31220511	3	83	theme	virus	478:482	arg1	NA					433:434	NA	433:434	NA	433:434	The primary target identity regions for the human immune system are hemagglutinin (HA) and neuraminidase (NA), two surface proteins of the influenza A virus.
31220511	3	83	theme	virus	478:482	arg1	HA					410:411	HA	410:411	HA	410:411	The primary target identity regions for the human immune system are hemagglutinin (HA) and neuraminidase (NA), two surface proteins of the influenza A virus.
31220511	0	84	theme	learning	63:70	arg1	methods					72:78	machine learning methods	55:78	machine learning methods of N-linked glycosylation sites and B-cell epitopes	55:130	Conservation region finding for influenza A viruses by machine learning methods of N-linked glycosylation sites and B-cell epitopes.
31220511	10	85	gly	glycosylation	1510:1522	arg2	sites					1524:1528	N-linked glycosylation sites	1501:1528	N-linked glycosylation sites	1501:1528	Compared to the prediction of the Immune Epitope Database (IEDB) antibody neutralization response (i.e., screening of antibody sequence regions), in this study, the virus sequence coverage was large and accurate and contained N-linked glycosylation sites.
31220511	1	86	theme	type	143:146	arg1	disease					172:178	a serious infectious disease	151:178	a serious infectious disease of the human respiratory tract	151:209	Influenza type A, a serious infectious disease of the human respiratory tract, poses an enormous threat to human health worldwide.
31220511	1	86	theme	type	143:146	arg1	A					148:148	Influenza type A	133:148	Influenza type A	133:148	Influenza type A, a serious infectious disease of the human respiratory tract, poses an enormous threat to human health worldwide.
31220511	11	87	theme	prediction	1607:1616	arg1	method					1618:1623	the machine learning-based prediction method	1580:1623	the machine learning-based prediction method	1580:1623	The results of this study proved that we can use the machine learning-based prediction method to solve the problem of vaccine invalidation that occurred during the rapid evolution of the influenza A virus and also as a prevaccine assessment.
31220511	0	88	theme	glycosylation	92:104	arg1	sites					106:110	N-linked glycosylation sites	83:110	N-linked glycosylation sites	83:110	Conservation region finding for influenza A viruses by machine learning methods of N-linked glycosylation sites and B-cell epitopes.
31220511	6	89	theme	influenza	878:886	arg1	database					894:901	the influenza virus database	874:901	the influenza virus database	874:901	On the basis of these three properties, we analyzed 12,832 HA and 9487 NA protein sequences, which we retrieved from the influenza virus database.
31220511	10	90	theme	Epitope	1316:1322	arg1	response					1364:1371	the Immune Epitope Database (IEDB) antibody neutralization response	1305:1371	the Immune Epitope Database (IEDB) antibody neutralization response (i.e., screening of antibody sequence regions)	1305:1418	Compared to the prediction of the Immune Epitope Database (IEDB) antibody neutralization response (i.e., screening of antibody sequence regions), in this study, the virus sequence coverage was large and accurate and contained N-linked glycosylation sites.
31220511	5	91	theme	ribonucleic	659:669	arg1	conservation					691:702	ribonucleic acid (RNA) sequence conservation	659:702	ribonucleic acid (RNA) sequence conservation	659:702	This study focused on three genetic features of viral surface proteins: ribonucleic acid (RNA) sequence conservation, linear B-cell epitopes, and N-linked glycosylation.
31220511	9	92	theme	conservation	1180:1191	arg1	regions					1193:1199	conservation regions	1180:1199	conservation regions with sequences showing linear B-cell epitopes and N-linked glycosylation	1180:1272	Furthermore, using machine learning methods, we looked for conservation regions with sequences showing linear B-cell epitopes and N-linked glycosylation.
31220511	10	93	theme	neutralization	1349:1362	arg1	response					1364:1371	the Immune Epitope Database (IEDB) antibody neutralization response	1305:1371	the Immune Epitope Database (IEDB) antibody neutralization response (i.e., screening of antibody sequence regions)	1305:1418	Compared to the prediction of the Immune Epitope Database (IEDB) antibody neutralization response (i.e., screening of antibody sequence regions), in this study, the virus sequence coverage was large and accurate and contained N-linked glycosylation sites.
31220511	10	94	dep	screening	1380:1388	arg1	i.e.					1374:1377	i.e.	1374:1377	i.e.	1374:1377	Compared to the prediction of the Immune Epitope Database (IEDB) antibody neutralization response (i.e., screening of antibody sequence regions), in this study, the virus sequence coverage was large and accurate and contained N-linked glycosylation sites.
31220511	3	95	theme	identity	346:353	arg1	hemagglutinin					395:407	hemagglutinin	395:407	hemagglutinin (HA)	395:412	The primary target identity regions for the human immune system are hemagglutinin (HA) and neuraminidase (NA), two surface proteins of the influenza A virus.
31220511	3	95	theme	identity	346:353	arg1	regions					355:361	The primary target identity regions	327:361	The primary target identity regions for the human immune system	327:389	The primary target identity regions for the human immune system are hemagglutinin (HA) and neuraminidase (NA), two surface proteins of the influenza A virus.
31220511	10	96	theme	N-linked	1501:1508	arg1	sites					1524:1528	N-linked glycosylation sites	1501:1528	N-linked glycosylation sites	1501:1528	Compared to the prediction of the Immune Epitope Database (IEDB) antibody neutralization response (i.e., screening of antibody sequence regions), in this study, the virus sequence coverage was large and accurate and contained N-linked glycosylation sites.
31220511	3	97	theme	primary	331:337	arg1	hemagglutinin					395:407	hemagglutinin	395:407	hemagglutinin (HA)	395:412	The primary target identity regions for the human immune system are hemagglutinin (HA) and neuraminidase (NA), two surface proteins of the influenza A virus.
31220511	3	97	theme	primary	331:337	arg1	regions					355:361	The primary target identity regions	327:361	The primary target identity regions for the human immune system	327:389	The primary target identity regions for the human immune system are hemagglutinin (HA) and neuraminidase (NA), two surface proteins of the influenza A virus.
31220511	12	98	used	used	1817:1820	arg2	reference					1862:1870	a universal influenza vaccine design reference	1825:1870	a universal influenza vaccine design reference in the future	1825:1884	In addition, the screening fragments can be used as a universal influenza vaccine design reference in the future.
31220511	12	98	used	used	1817:1820	arg2	fragments					1800:1808	the screening fragments	1786:1808	the screening fragments	1786:1808	In addition, the screening fragments can be used as a universal influenza vaccine design reference in the future.
31220511	0	99	theme	A	42:42	arg1	viruses					44:50	influenza A viruses	32:50	influenza A viruses	32:50	Conservation region finding for influenza A viruses by machine learning methods of N-linked glycosylation sites and B-cell epitopes.
31220511	9	100	theme	B-cell	1231:1236	arg1	epitopes					1238:1245	linear B-cell epitopes	1224:1245	linear B-cell epitopes	1224:1245	Furthermore, using machine learning methods, we looked for conservation regions with sequences showing linear B-cell epitopes and N-linked glycosylation.
31220511	3	101	theme	human	371:375	arg1	system					384:389	the human immune system	367:389	the human immune system	367:389	The primary target identity regions for the human immune system are hemagglutinin (HA) and neuraminidase (NA), two surface proteins of the influenza A virus.
31220511	12	102	theme	screening	1790:1798	arg1	reference					1862:1870	a universal influenza vaccine design reference	1825:1870	a universal influenza vaccine design reference in the future	1825:1884	In addition, the screening fragments can be used as a universal influenza vaccine design reference in the future.
31220511	12	102	theme	screening	1790:1798	arg1	fragments					1800:1808	the screening fragments	1786:1808	the screening fragments	1786:1808	In addition, the screening fragments can be used as a universal influenza vaccine design reference in the future.
31220511	11	103	theme	study	1551:1555	arg1	results					1535:1541	The results	1531:1541	The results of this study	1531:1555	The results of this study proved that we can use the machine learning-based prediction method to solve the problem of vaccine invalidation that occurred during the rapid evolution of the influenza A virus and also as a prevaccine assessment.
31220511	0	104	theme	sites	106:110	arg1	methods					72:78	machine learning methods	55:78	machine learning methods of N-linked glycosylation sites and B-cell epitopes	55:130	Conservation region finding for influenza A viruses by machine learning methods of N-linked glycosylation sites and B-cell epitopes.
31220511	5	105	theme	linear	705:710	arg1	epitopes					719:726	linear B-cell epitopes	705:726	linear B-cell epitopes	705:726	This study focused on three genetic features of viral surface proteins: ribonucleic acid (RNA) sequence conservation, linear B-cell epitopes, and N-linked glycosylation.
31220511	10	106	theme	sequence	1402:1409	arg1	regions					1411:1417	antibody sequence regions	1393:1417	antibody sequence regions	1393:1417	Compared to the prediction of the Immune Epitope Database (IEDB) antibody neutralization response (i.e., screening of antibody sequence regions), in this study, the virus sequence coverage was large and accurate and contained N-linked glycosylation sites.
31220511	11	107	theme	A	1728:1728	arg1	virus					1730:1734	the influenza A virus	1714:1734	the influenza A virus	1714:1734	The results of this study proved that we can use the machine learning-based prediction method to solve the problem of vaccine invalidation that occurred during the rapid evolution of the influenza A virus and also as a prevaccine assessment.
31220511	10	108	from	study	1429:1433	arg1	large					1468:1472	large	1468:1472	large	1468:1472	Compared to the prediction of the Immune Epitope Database (IEDB) antibody neutralization response (i.e., screening of antibody sequence regions), in this study, the virus sequence coverage was large and accurate and contained N-linked glycosylation sites.
31220511	10	108	from	study	1429:1433	arg1	coverage					1455:1462	the virus sequence coverage	1436:1462	the virus sequence coverage	1436:1462	Compared to the prediction of the Immune Epitope Database (IEDB) antibody neutralization response (i.e., screening of antibody sequence regions), in this study, the virus sequence coverage was large and accurate and contained N-linked glycosylation sites.
31220511	1	109	theme	enormous	221:228	arg1	threat					230:235	an enormous threat	218:235	an enormous threat to human health worldwide	218:261	Influenza type A, a serious infectious disease of the human respiratory tract, poses an enormous threat to human health worldwide.
29951115	9	0	from	plants	1469:1474	arg1	straw					1451:1455	straw	1451:1455	straw from mutant plants	1451:1474	Enzymatic saccharification assays showed that straw from mutant plants was marginally more degradable following a 190 °C hydrothermal pretreatment, while there were no differences without or after a 120 °C hydrothermal pretreatment.
29951115	4	1	theme	Different	576:584	arg1	components					596:605	Different cell wall components	576:605	Different cell wall components	576:605	Different cell wall components have been proposed to interact with the silica pool in plant shoots, but the understanding of the underlying processes is still limited.
29951115	3	2	theme	biomass	484:490	arg1	engineering					492:502	biomass engineering	484:502	biomass engineering	484:502	Solutions to reduce the silicon content either by biomass engineering or development of downstream separation methods are therefore targeted.
29951115	4	3	theme	wall	591:594	arg1	components					596:605	Different cell wall components	576:605	Different cell wall components	576:605	Different cell wall components have been proposed to interact with the silica pool in plant shoots, but the understanding of the underlying processes is still limited.
29951115	3	4	theme	separation	533:542	arg1	methods					544:550	downstream separation methods	522:550	downstream separation methods	522:550	Solutions to reduce the silicon content either by biomass engineering or development of downstream separation methods are therefore targeted.
29951115	1	5	theme	adverse	253:259	arg1	effects					261:267	adverse effects	253:267	adverse effects of biotic and abiotic stress	253:296	BACKGROUND Plants and in particular grasses benefit from a high uptake of silicon (Si) which improves their growth and productivity by alleviating adverse effects of biotic and abiotic stress.
29951115	4	6	theme	plant	662:666	arg1	shoots					668:673	plant shoots	662:673	plant shoots	662:673	Different cell wall components have been proposed to interact with the silica pool in plant shoots, but the understanding of the underlying processes is still limited.
29951115	11	7	theme	silicon	1846:1852	arg1	concentration					1854:1866	the reduced silicon concentration	1834:1866	the reduced silicon concentration	1834:1866	The modifications of the cell wall network and the reduced silicon concentration appear to have little or no implications on biomass recalcitrance to enzymatic saccharification.
29951115	7	8	theme	element	1089:1095	arg1	deposition					1070:1079	deposition	1070:1079	deposition of this element	1070:1095	In the mutant defective in silicon uptake, low silicon availability favoured deposition of this element in the amorphous form or bound to cell wall polymers rather than as silicified structures.
29951115	1	9	theme	silicon	180:186	arg1	uptake					170:175	a high uptake	163:175	a high uptake of silicon (Si) which improves their growth and productivity by alleviating adverse effects of biotic and abiotic stress	163:296	BACKGROUND Plants and in particular grasses benefit from a high uptake of silicon (Si) which improves their growth and productivity by alleviating adverse effects of biotic and abiotic stress.
29951115	8	10	theme	mutant	1274:1279	arg1	plants					1281:1286	the mutant plants	1270:1286	the mutant plants	1270:1286	Several alterations in non-cellulosic polysaccharides and lignin were recorded in the mutant plants, indicating differences in the types of linkages and in the three-dimensional organization of the cell wall network.
29951115	2	11	contain	have	349:352	arg1	present					320:326	the silicon present	308:326	the silicon present in plant tissues	308:343	However, the silicon present in plant tissues may have a negative impact on the processing and degradation of lignocellulosic biomass.
29951115	2	11	contain	have	349:352	arg2	impact					365:370	a negative impact	354:370	a negative impact	354:370	However, the silicon present in plant tissues may have a negative impact on the processing and degradation of lignocellulosic biomass.
29951115	10	12	theme	polymers	1766:1773	arg1	linkages					1739:1746	linkages	1739:1746	linkages of non-cellulosic polymers and lignin	1739:1784	CONCLUSIONS We conclude that silicon affects the composition of plant cell walls, mostly by altering linkages of non-cellulosic polymers and lignin.
29951115	5	13	theme	cell	819:822	arg1	composition					829:839	cell wall composition	819:839	cell wall composition	819:839	RESULTS In the present study, we have characterized silicon deposition and cell wall composition in Brachypodium distachyon wild-type and low-silicon 1 (Bdlsi1-1) mutant plants.
29951115	8	14	theme	non-cellulosic	1211:1224	arg1	polysaccharides					1226:1240	non-cellulosic polysaccharides	1211:1240	non-cellulosic polysaccharides	1211:1240	Several alterations in non-cellulosic polysaccharides and lignin were recorded in the mutant plants, indicating differences in the types of linkages and in the three-dimensional organization of the cell wall network.
29951115	6	15	theme	different	944:952	arg1	organs					954:959	different organs	944:959	different organs	944:959	Our analyses included different organs and plant developmental stages.
29951115	11	16	theme	enzymatic	1937:1945	arg1	saccharification					1947:1962	enzymatic saccharification	1937:1962	enzymatic saccharification	1937:1962	The modifications of the cell wall network and the reduced silicon concentration appear to have little or no implications on biomass recalcitrance to enzymatic saccharification.
29951115	7	17	from	defective	1007:1015	arg1	uptake					1028:1033	silicon uptake	1020:1033	silicon uptake	1020:1033	In the mutant defective in silicon uptake, low silicon availability favoured deposition of this element in the amorphous form or bound to cell wall polymers rather than as silicified structures.
29951115	5	18	theme	silicon	796:802	arg1	deposition					804:813	silicon deposition	796:813	silicon deposition	796:813	RESULTS In the present study, we have characterized silicon deposition and cell wall composition in Brachypodium distachyon wild-type and low-silicon 1 (Bdlsi1-1) mutant plants.
29951115	7	19	theme	defective	1007:1015	arg1	mutant					1000:1005	the mutant	996:1005	the mutant defective in silicon uptake	996:1033	In the mutant defective in silicon uptake, low silicon availability favoured deposition of this element in the amorphous form or bound to cell wall polymers rather than as silicified structures.
29951115	7	20	theme	low silicon	1036:1046	arg1	availability					1048:1059	low silicon availability	1036:1059	low silicon availability	1036:1059	In the mutant defective in silicon uptake, low silicon availability favoured deposition of this element in the amorphous form or bound to cell wall polymers rather than as silicified structures.
29951115	9	21	theme	hydrothermal	1526:1537	arg1	pretreatment					1539:1550	a 190 °C hydrothermal pretreatment	1517:1550	a 190 °C hydrothermal pretreatment	1517:1550	Enzymatic saccharification assays showed that straw from mutant plants was marginally more degradable following a 190 °C hydrothermal pretreatment, while there were no differences without or after a 120 °C hydrothermal pretreatment.
29951115	4	22	theme	underlying	705:714	arg1	processes					716:724	the underlying processes	701:724	the underlying processes	701:724	Different cell wall components have been proposed to interact with the silica pool in plant shoots, but the understanding of the underlying processes is still limited.
29951115	1	23	theme	particular	131:140	arg1	grasses					142:148	particular grasses	131:148	particular grasses	131:148	BACKGROUND Plants and in particular grasses benefit from a high uptake of silicon (Si) which improves their growth and productivity by alleviating adverse effects of biotic and abiotic stress.
29951115	5	24	theme	mutant	907:912	arg1	plants					914:919	Brachypodium distachyon wild-type and low-silicon 1 (Bdlsi1-1) mutant plants	844:919	Brachypodium distachyon wild-type and low-silicon 1 (Bdlsi1-1) mutant plants	844:919	RESULTS In the present study, we have characterized silicon deposition and cell wall composition in Brachypodium distachyon wild-type and low-silicon 1 (Bdlsi1-1) mutant plants.
29951115	11	25	theme	network	1822:1828	arg1	modifications					1791:1803	The modifications	1787:1803	The modifications of the cell wall network and the reduced silicon concentration	1787:1866	The modifications of the cell wall network and the reduced silicon concentration appear to have little or no implications on biomass recalcitrance to enzymatic saccharification.
29951115	11	26	theme	cell	1812:1815	arg1	network					1822:1828	the cell wall network	1808:1828	the cell wall network	1808:1828	The modifications of the cell wall network and the reduced silicon concentration appear to have little or no implications on biomass recalcitrance to enzymatic saccharification.
29951115	0	27	theme	Brachypodium	81:92	arg1	distachyon					94:103	Brachypodium distachyon	81:103	Brachypodium distachyon	81:103	The impact of silicon on cell wall composition and enzymatic saccharification of Brachypodium distachyon.
29951115	8	28	from	differences	1300:1310	arg1	organization					1366:1377	the three-dimensional organization	1344:1377	the three-dimensional organization of the cell wall network	1344:1402	Several alterations in non-cellulosic polysaccharides and lignin were recorded in the mutant plants, indicating differences in the types of linkages and in the three-dimensional organization of the cell wall network.
29951115	8	28	from	differences	1300:1310	arg1	types					1319:1323	the types	1315:1323	the types of linkages	1315:1335	Several alterations in non-cellulosic polysaccharides and lignin were recorded in the mutant plants, indicating differences in the types of linkages and in the three-dimensional organization of the cell wall network.
29951115	6	29	theme	developmental	971:983	arg1	stages					985:990	plant developmental stages	965:990	plant developmental stages	965:990	Our analyses included different organs and plant developmental stages.
29951115	10	30	theme	cell	1708:1711	arg1	walls					1713:1717	plant cell walls	1702:1717	plant cell walls	1702:1717	CONCLUSIONS We conclude that silicon affects the composition of plant cell walls, mostly by altering linkages of non-cellulosic polymers and lignin.
29951115	7	31	theme	wall	1136:1139	arg1	polymers					1141:1148	cell wall polymers	1131:1148	cell wall polymers	1131:1148	In the mutant defective in silicon uptake, low silicon availability favoured deposition of this element in the amorphous form or bound to cell wall polymers rather than as silicified structures.
29951115	2	32	theme	biomass	425:431	arg1	degradation					394:404	degradation	394:404	degradation	394:404	However, the silicon present in plant tissues may have a negative impact on the processing and degradation of lignocellulosic biomass.
29951115	2	32	theme	biomass	425:431	arg1	processing					379:388	processing	379:388	processing	379:388	However, the silicon present in plant tissues may have a negative impact on the processing and degradation of lignocellulosic biomass.
29951115	8	33	theme	the cell	1382:1389	arg1	network					1396:1402	the cell wall network	1382:1402	the cell wall network	1382:1402	Several alterations in non-cellulosic polysaccharides and lignin were recorded in the mutant plants, indicating differences in the types of linkages and in the three-dimensional organization of the cell wall network.
29951115	7	34	theme	silicified	1165:1174	arg1	structures					1176:1185	silicified structures	1165:1185	silicified structures	1165:1185	In the mutant defective in silicon uptake, low silicon availability favoured deposition of this element in the amorphous form or bound to cell wall polymers rather than as silicified structures.
29951115	1	35	from	Plants	117:122	arg1	grasses					142:148	particular grasses	131:148	particular grasses	131:148	BACKGROUND Plants and in particular grasses benefit from a high uptake of silicon (Si) which improves their growth and productivity by alleviating adverse effects of biotic and abiotic stress.
29951115	9	36	theme	120 °C	1604:1609	arg1	pretreatment					1624:1635	a 120 °C hydrothermal pretreatment	1602:1635	a 120 °C hydrothermal pretreatment	1602:1635	Enzymatic saccharification assays showed that straw from mutant plants was marginally more degradable following a 190 °C hydrothermal pretreatment, while there were no differences without or after a 120 °C hydrothermal pretreatment.
29951115	8	37	theme	network	1396:1402	arg1	organization					1366:1377	the three-dimensional organization	1344:1377	the three-dimensional organization of the cell wall network	1344:1402	Several alterations in non-cellulosic polysaccharides and lignin were recorded in the mutant plants, indicating differences in the types of linkages and in the three-dimensional organization of the cell wall network.
29951115	1	38	theme	biotic	272:277	arg1	stress					291:296	biotic and abiotic stress	272:296	biotic and abiotic stress	272:296	BACKGROUND Plants and in particular grasses benefit from a high uptake of silicon (Si) which improves their growth and productivity by alleviating adverse effects of biotic and abiotic stress.
29951115	1	39	theme	BACKGROUND	106:115	arg1	Plants					117:122	BACKGROUND Plants	106:122	BACKGROUND Plants and in particular grasses	106:148	BACKGROUND Plants and in particular grasses benefit from a high uptake of silicon (Si) which improves their growth and productivity by alleviating adverse effects of biotic and abiotic stress.
29951115	9	40	theme	mutant	1462:1467	arg1	plants					1469:1474	mutant plants	1462:1474	mutant plants	1462:1474	Enzymatic saccharification assays showed that straw from mutant plants was marginally more degradable following a 190 °C hydrothermal pretreatment, while there were no differences without or after a 120 °C hydrothermal pretreatment.
29951115	1	41	theme	abiotic	283:289	arg1	stress					291:296	biotic and abiotic stress	272:296	biotic and abiotic stress	272:296	BACKGROUND Plants and in particular grasses benefit from a high uptake of silicon (Si) which improves their growth and productivity by alleviating adverse effects of biotic and abiotic stress.
29951115	10	42	theme	non-cellulosic	1751:1764	arg1	polymers					1766:1773	non-cellulosic polymers	1751:1773	non-cellulosic polymers	1751:1773	CONCLUSIONS We conclude that silicon affects the composition of plant cell walls, mostly by altering linkages of non-cellulosic polymers and lignin.
29951115	0	43	theme	wall	30:33	arg1	composition					35:45	cell wall composition	25:45	cell wall composition	25:45	The impact of silicon on cell wall composition and enzymatic saccharification of Brachypodium distachyon.
29951115	11	44	theme	biomass	1912:1918	arg1	recalcitrance					1920:1932	biomass recalcitrance	1912:1932	biomass recalcitrance to enzymatic saccharification	1912:1962	The modifications of the cell wall network and the reduced silicon concentration appear to have little or no implications on biomass recalcitrance to enzymatic saccharification.
29951115	3	45	dep	Solutions	434:442	arg1	reduce					447:452	reduce	447:452	to reduce the silicon content either by biomass engineering or development of downstream separation methods	444:550	Solutions to reduce the silicon content either by biomass engineering or development of downstream separation methods are therefore targeted.
29951115	7	46	from	uptake	1028:1033	arg1	defective					1007:1015	defective	1007:1015	defective	1007:1015	In the mutant defective in silicon uptake, low silicon availability favoured deposition of this element in the amorphous form or bound to cell wall polymers rather than as silicified structures.
29951115	9	47	theme	saccharification	1415:1430	arg1	assays					1432:1437	Enzymatic saccharification assays	1405:1437	Enzymatic saccharification assays	1405:1437	Enzymatic saccharification assays showed that straw from mutant plants was marginally more degradable following a 190 °C hydrothermal pretreatment, while there were no differences without or after a 120 °C hydrothermal pretreatment.
29951115	8	48	from	alterations	1196:1206	arg1	lignin					1246:1251	lignin	1246:1251	lignin	1246:1251	Several alterations in non-cellulosic polysaccharides and lignin were recorded in the mutant plants, indicating differences in the types of linkages and in the three-dimensional organization of the cell wall network.
29951115	8	48	from	alterations	1196:1206	arg1	polysaccharides					1226:1240	non-cellulosic polysaccharides	1211:1240	non-cellulosic polysaccharides	1211:1240	Several alterations in non-cellulosic polysaccharides and lignin were recorded in the mutant plants, indicating differences in the types of linkages and in the three-dimensional organization of the cell wall network.
29951115	2	49	theme	plant	331:335	arg1	tissues					337:343	plant tissues	331:343	plant tissues	331:343	However, the silicon present in plant tissues may have a negative impact on the processing and degradation of lignocellulosic biomass.
29951115	7	50	theme	amorphous	1104:1112	arg1	form					1114:1117	the amorphous form	1100:1117	the amorphous form	1100:1117	In the mutant defective in silicon uptake, low silicon availability favoured deposition of this element in the amorphous form or bound to cell wall polymers rather than as silicified structures.
29951115	3	51	theme	downstream	522:531	arg1	methods					544:550	downstream separation methods	522:550	downstream separation methods	522:550	Solutions to reduce the silicon content either by biomass engineering or development of downstream separation methods are therefore targeted.
29951115	2	52	from	present	320:326	arg1	tissues					337:343	plant tissues	331:343	plant tissues	331:343	However, the silicon present in plant tissues may have a negative impact on the processing and degradation of lignocellulosic biomass.
29951115	4	53	theme	cell	586:589	arg1	components					596:605	Different cell wall components	576:605	Different cell wall components	576:605	Different cell wall components have been proposed to interact with the silica pool in plant shoots, but the understanding of the underlying processes is still limited.
29951115	10	54	dep	CONCLUSIONS	1638:1648	arg1	conclude					1653:1660	conclude	1653:1660	conclude that silicon affects the composition of plant cell walls, mostly by altering linkages of non-cellulosic polymers and lignin	1653:1784	CONCLUSIONS We conclude that silicon affects the composition of plant cell walls, mostly by altering linkages of non-cellulosic polymers and lignin.
29951115	1	55	theme	high	165:168	arg1	uptake					170:175	a high uptake	163:175	a high uptake of silicon (Si) which improves their growth and productivity by alleviating adverse effects of biotic and abiotic stress	163:296	BACKGROUND Plants and in particular grasses benefit from a high uptake of silicon (Si) which improves their growth and productivity by alleviating adverse effects of biotic and abiotic stress.
29951115	7	56	theme	cell	1131:1134	arg1	polymers					1141:1148	cell wall polymers	1131:1148	cell wall polymers	1131:1148	In the mutant defective in silicon uptake, low silicon availability favoured deposition of this element in the amorphous form or bound to cell wall polymers rather than as silicified structures.
29951115	4	57	theme	silica	647:652	arg1	pool					654:657	the silica pool	643:657	the silica pool	643:657	Different cell wall components have been proposed to interact with the silica pool in plant shoots, but the understanding of the underlying processes is still limited.
29951115	6	58	theme	plant	965:969	arg1	stages					985:990	plant developmental stages	965:990	plant developmental stages	965:990	Our analyses included different organs and plant developmental stages.
29951115	11	59	theme	concentration	1854:1866	arg1	modifications					1791:1803	The modifications	1787:1803	The modifications of the cell wall network and the reduced silicon concentration	1787:1866	The modifications of the cell wall network and the reduced silicon concentration appear to have little or no implications on biomass recalcitrance to enzymatic saccharification.
29951115	3	60	theme	methods	544:550	arg1	development					507:517	development	507:517	development	507:517	Solutions to reduce the silicon content either by biomass engineering or development of downstream separation methods are therefore targeted.
29951115	3	60	theme	methods	544:550	arg1	engineering					492:502	biomass engineering	484:502	biomass engineering	484:502	Solutions to reduce the silicon content either by biomass engineering or development of downstream separation methods are therefore targeted.
29951115	8	61	theme	Several	1188:1194	arg1	alterations					1196:1206	Several alterations	1188:1206	Several alterations in non-cellulosic polysaccharides and lignin	1188:1251	Several alterations in non-cellulosic polysaccharides and lignin were recorded in the mutant plants, indicating differences in the types of linkages and in the three-dimensional organization of the cell wall network.
29951115	11	62	theme	reduced	1838:1844	arg1	concentration					1854:1866	the reduced silicon concentration	1834:1866	the reduced silicon concentration	1834:1866	The modifications of the cell wall network and the reduced silicon concentration appear to have little or no implications on biomass recalcitrance to enzymatic saccharification.
29951115	5	63	theme	wild-type	868:876	arg1	plants					914:919	Brachypodium distachyon wild-type and low-silicon 1 (Bdlsi1-1) mutant plants	844:919	Brachypodium distachyon wild-type and low-silicon 1 (Bdlsi1-1) mutant plants	844:919	RESULTS In the present study, we have characterized silicon deposition and cell wall composition in Brachypodium distachyon wild-type and low-silicon 1 (Bdlsi1-1) mutant plants.
29951115	5	64	theme	wall	824:827	arg1	composition					829:839	cell wall composition	819:839	cell wall composition	819:839	RESULTS In the present study, we have characterized silicon deposition and cell wall composition in Brachypodium distachyon wild-type and low-silicon 1 (Bdlsi1-1) mutant plants.
29951115	5	65	dep	RESULTS	744:750	arg1	characterized					782:794	characterized	782:794	have characterized silicon deposition and cell wall composition in Brachypodium distachyon wild-type and low-silicon 1 (Bdlsi1-1) mutant plants	777:919	RESULTS In the present study, we have characterized silicon deposition and cell wall composition in Brachypodium distachyon wild-type and low-silicon 1 (Bdlsi1-1) mutant plants.
29951115	5	66	from	composition	829:839	arg1	plants					914:919	Brachypodium distachyon wild-type and low-silicon 1 (Bdlsi1-1) mutant plants	844:919	Brachypodium distachyon wild-type and low-silicon 1 (Bdlsi1-1) mutant plants	844:919	RESULTS In the present study, we have characterized silicon deposition and cell wall composition in Brachypodium distachyon wild-type and low-silicon 1 (Bdlsi1-1) mutant plants.
29951115	9	67	theme	190 °C	1519:1524	arg1	pretreatment					1539:1550	a 190 °C hydrothermal pretreatment	1517:1550	a 190 °C hydrothermal pretreatment	1517:1550	Enzymatic saccharification assays showed that straw from mutant plants was marginally more degradable following a 190 °C hydrothermal pretreatment, while there were no differences without or after a 120 °C hydrothermal pretreatment.
29951115	7	68	theme	silicon	1020:1026	arg1	uptake					1028:1033	silicon uptake	1020:1033	silicon uptake	1020:1033	In the mutant defective in silicon uptake, low silicon availability favoured deposition of this element in the amorphous form or bound to cell wall polymers rather than as silicified structures.
29951115	0	69	theme	cell	25:28	arg1	composition					35:45	cell wall composition	25:45	cell wall composition	25:45	The impact of silicon on cell wall composition and enzymatic saccharification of Brachypodium distachyon.
29951115	0	70	theme	enzymatic	51:59	arg1	saccharification					61:76	enzymatic saccharification	51:76	enzymatic saccharification of Brachypodium distachyon	51:103	The impact of silicon on cell wall composition and enzymatic saccharification of Brachypodium distachyon.
29951115	0	71	from	impact	4:9	arg1	saccharification					61:76	enzymatic saccharification	51:76	enzymatic saccharification of Brachypodium distachyon	51:103	The impact of silicon on cell wall composition and enzymatic saccharification of Brachypodium distachyon.
29951115	0	71	from	impact	4:9	arg1	composition					35:45	cell wall composition	25:45	cell wall composition	25:45	The impact of silicon on cell wall composition and enzymatic saccharification of Brachypodium distachyon.
29951115	8	72	theme	linkages	1328:1335	arg1	types					1319:1323	the types	1315:1323	the types of linkages	1315:1335	Several alterations in non-cellulosic polysaccharides and lignin were recorded in the mutant plants, indicating differences in the types of linkages and in the three-dimensional organization of the cell wall network.
29951115	0	73	theme	distachyon	94:103	arg1	saccharification					61:76	enzymatic saccharification	51:76	enzymatic saccharification of Brachypodium distachyon	51:103	The impact of silicon on cell wall composition and enzymatic saccharification of Brachypodium distachyon.
29951115	0	73	theme	distachyon	94:103	arg1	composition					35:45	cell wall composition	25:45	cell wall composition	25:45	The impact of silicon on cell wall composition and enzymatic saccharification of Brachypodium distachyon.
29951115	11	74	theme	wall	1817:1820	arg1	network					1822:1828	the cell wall network	1808:1828	the cell wall network	1808:1828	The modifications of the cell wall network and the reduced silicon concentration appear to have little or no implications on biomass recalcitrance to enzymatic saccharification.
29951115	5	75	from	deposition	804:813	arg1	plants					914:919	Brachypodium distachyon wild-type and low-silicon 1 (Bdlsi1-1) mutant plants	844:919	Brachypodium distachyon wild-type and low-silicon 1 (Bdlsi1-1) mutant plants	844:919	RESULTS In the present study, we have characterized silicon deposition and cell wall composition in Brachypodium distachyon wild-type and low-silicon 1 (Bdlsi1-1) mutant plants.
29951115	8	76	theme	three-dimensional	1348:1364	arg1	organization					1366:1377	the three-dimensional organization	1344:1377	the three-dimensional organization of the cell wall network	1344:1402	Several alterations in non-cellulosic polysaccharides and lignin were recorded in the mutant plants, indicating differences in the types of linkages and in the three-dimensional organization of the cell wall network.
29951115	10	77	theme	plant	1702:1706	arg1	walls					1713:1717	plant cell walls	1702:1717	plant cell walls	1702:1717	CONCLUSIONS We conclude that silicon affects the composition of plant cell walls, mostly by altering linkages of non-cellulosic polymers and lignin.
29951115	2	78	theme	lignocellulosic	409:423	arg1	biomass					425:431	lignocellulosic biomass	409:431	lignocellulosic biomass	409:431	However, the silicon present in plant tissues may have a negative impact on the processing and degradation of lignocellulosic biomass.
29951115	4	79	theme	processes	716:724	arg1	understanding					684:696	the understanding	680:696	the understanding of the underlying processes	680:724	Different cell wall components have been proposed to interact with the silica pool in plant shoots, but the understanding of the underlying processes is still limited.
29951115	0	80	theme	silicon	14:20	arg1	impact					4:9	The impact	0:9	The impact of silicon on cell wall composition and enzymatic saccharification of Brachypodium distachyon.	0:104	The impact of silicon on cell wall composition and enzymatic saccharification of Brachypodium distachyon.
29951115	9	81	theme	hydrothermal	1611:1622	arg1	pretreatment					1624:1635	a 120 °C hydrothermal pretreatment	1602:1635	a 120 °C hydrothermal pretreatment	1602:1635	Enzymatic saccharification assays showed that straw from mutant plants was marginally more degradable following a 190 °C hydrothermal pretreatment, while there were no differences without or after a 120 °C hydrothermal pretreatment.
29951115	8	82	theme	wall	1391:1394	arg1	network					1396:1402	the cell wall network	1382:1402	the cell wall network	1382:1402	Several alterations in non-cellulosic polysaccharides and lignin were recorded in the mutant plants, indicating differences in the types of linkages and in the three-dimensional organization of the cell wall network.
29951115	5	83	theme	present	759:765	arg1	study					767:771	the present study	755:771	the present study	755:771	RESULTS In the present study, we have characterized silicon deposition and cell wall composition in Brachypodium distachyon wild-type and low-silicon 1 (Bdlsi1-1) mutant plants.
29951115	10	84	theme	walls	1713:1717	arg1	composition					1687:1697	the composition	1683:1697	the composition of plant cell walls	1683:1717	CONCLUSIONS We conclude that silicon affects the composition of plant cell walls, mostly by altering linkages of non-cellulosic polymers and lignin.
29951115	1	85	theme	stress	291:296	arg1	effects					261:267	adverse effects	253:267	adverse effects of biotic and abiotic stress	253:296	BACKGROUND Plants and in particular grasses benefit from a high uptake of silicon (Si) which improves their growth and productivity by alleviating adverse effects of biotic and abiotic stress.
29951115	2	86	dep	processing	379:388	arg1	the					375:377	the	375:377	the	375:377	However, the silicon present in plant tissues may have a negative impact on the processing and degradation of lignocellulosic biomass.
29951115	2	87	theme	silicon	312:318	arg1	present					320:326	the silicon present	308:326	the silicon present in plant tissues	308:343	However, the silicon present in plant tissues may have a negative impact on the processing and degradation of lignocellulosic biomass.
29951115	11	88	contain	have	1878:1881	arg2	little					1883:1888	little	1883:1888	little	1883:1888	The modifications of the cell wall network and the reduced silicon concentration appear to have little or no implications on biomass recalcitrance to enzymatic saccharification.
29951115	11	88	contain	have	1878:1881	arg1	modifications					1791:1803	The modifications	1787:1803	The modifications of the cell wall network and the reduced silicon concentration	1787:1866	The modifications of the cell wall network and the reduced silicon concentration appear to have little or no implications on biomass recalcitrance to enzymatic saccharification.
29951115	11	88	contain	have	1878:1881	arg2	implications					1896:1907	no implications	1893:1907	no implications	1893:1907	The modifications of the cell wall network and the reduced silicon concentration appear to have little or no implications on biomass recalcitrance to enzymatic saccharification.
29951115	3	89	theme	silicon	458:464	arg1	content					466:472	the silicon content	454:472	the silicon content	454:472	Solutions to reduce the silicon content either by biomass engineering or development of downstream separation methods are therefore targeted.
29951115	9	90	theme	Enzymatic	1405:1413	arg1	assays					1432:1437	Enzymatic saccharification assays	1405:1437	Enzymatic saccharification assays	1405:1437	Enzymatic saccharification assays showed that straw from mutant plants was marginally more degradable following a 190 °C hydrothermal pretreatment, while there were no differences without or after a 120 °C hydrothermal pretreatment.
29951115	2	91	theme	negative	356:363	arg1	impact					365:370	a negative impact	354:370	a negative impact	354:370	However, the silicon present in plant tissues may have a negative impact on the processing and degradation of lignocellulosic biomass.
29951115	10	92	theme	lignin	1779:1784	arg1	linkages					1739:1746	linkages	1739:1746	linkages of non-cellulosic polymers and lignin	1739:1784	CONCLUSIONS We conclude that silicon affects the composition of plant cell walls, mostly by altering linkages of non-cellulosic polymers and lignin.
31068961	7	0	theme	proteins	1174:1181	arg1	presence					1102:1109	the presence	1098:1109	the presence of pectin, mucilage, lipids, terpenes, phenolic compounds, and proteins	1098:1181	Histochemical tests further revealed the presence of pectin, mucilage, lipids, terpenes, phenolic compounds, and proteins.
31068961	3	1	theme	abundant	475:482	arg1	secretion					495:503	an abundant gelatinous secretion	472:503	an abundant gelatinous secretion that covers almost all of its inflorescences	472:548	Elleanthus brasiliensis is distinguished by the presence of an abundant gelatinous secretion that covers almost all of its inflorescences.
31068961	1	2	theme	mucilage	247:254	arg1	mixture					236:242	a mixture	234:242	a mixture of mucilage, lipids, terpenes, and phenolic compounds	234:296	Colleters are trichomes or emergencies that produce a sticky exudate consisting of a mixture of mucilage, lipids, terpenes, and phenolic compounds.
31068961	10	3	from	common	1544:1549	arg1	inflorescences					1558:1571	the inflorescences	1554:1571	the inflorescences of tropical orchids	1554:1591	The occurrence of colleters in both species studied and in other orchids described in the literature suggests that these structures are common in the inflorescences of tropical orchids.
31068961	7	4	theme	Histochemical	1061:1073	arg1	tests					1075:1079	Histochemical tests	1061:1079	Histochemical tests	1061:1079	Histochemical tests further revealed the presence of pectin, mucilage, lipids, terpenes, phenolic compounds, and proteins.
31068961	1	5	theme	lipids	257:262	arg1	mixture					236:242	a mixture	234:242	a mixture of mucilage, lipids, terpenes, and phenolic compounds	234:296	Colleters are trichomes or emergencies that produce a sticky exudate consisting of a mixture of mucilage, lipids, terpenes, and phenolic compounds.
31068961	4	6	theme	chemical	680:687	arg1	composition					689:699	the chemical composition	676:699	the chemical composition of their secretion to better understand the functions of these secretory structures	676:783	We aimed to describe the histology of colleters in inflorescences of E. brasiliensis and Elleanthus crinipes, and to analyze the chemical composition of their secretion to better understand the functions of these secretory structures.
31068961	4	7	theme	crinipes	651:658	arg1	inflorescences					602:615	inflorescences	602:615	inflorescences of E. brasiliensis and Elleanthus crinipes	602:658	We aimed to describe the histology of colleters in inflorescences of E. brasiliensis and Elleanthus crinipes, and to analyze the chemical composition of their secretion to better understand the functions of these secretory structures.
31068961	8	8	attach	presence	1217:1224	arg1	secretion					1291:1299	the secretion	1287:1299	the secretion of E. brasiliensis	1287:1318	The GC-MS analysis confirmed the presence of γ-sitosterol and palmitic, linoleic, and stearic acids in the secretion of E. brasiliensis.
31068961	8	8	attach	presence	1217:1224	arg2	acids					1278:1282	palmitic, linoleic, and stearic acids	1246:1282	palmitic, linoleic, and stearic acids	1246:1282	The GC-MS analysis confirmed the presence of γ-sitosterol and palmitic, linoleic, and stearic acids in the secretion of E. brasiliensis.
31068961	8	8	attach	presence	1217:1224	arg2	γ-sitosterol					1229:1240	γ-sitosterol	1229:1240	γ-sitosterol	1229:1240	The GC-MS analysis confirmed the presence of γ-sitosterol and palmitic, linoleic, and stearic acids in the secretion of E. brasiliensis.
31068961	5	9	theme	secretion	844:852	arg1	colleters					814:822	colleters	814:822	colleters	814:822	Due to the low frequency of colleters and lack of visible secretion in E. crinipes, histochemical tests and chemical analyses were not performed for this species.
31068961	5	9	theme	secretion	844:852	arg1	lack					828:831	lack	828:831	lack of visible secretion in E. crinipes	828:867	Due to the low frequency of colleters and lack of visible secretion in E. crinipes, histochemical tests and chemical analyses were not performed for this species.
31068961	12	10	theme	secretions	1893:1902	arg1	diversity					1876:1884	the morphological and chemical diversity	1845:1884	the morphological and chemical diversity of the secretions produced by orchid colleters	1845:1931	This information broadens our knowledge of the morphological and chemical diversity of the secretions produced by orchid colleters.
31068961	9	11	theme	possible	1353:1360	arg1	presence					1362:1369	the possible presence	1349:1369	the possible presence of polysaccharides in the secretion	1349:1405	Infrared analysis indicated the possible presence of polysaccharides in the secretion.
31068961	7	12	theme	pectin	1114:1119	arg1	presence					1102:1109	the presence	1098:1109	the presence of pectin, mucilage, lipids, terpenes, phenolic compounds, and proteins	1098:1181	Histochemical tests further revealed the presence of pectin, mucilage, lipids, terpenes, phenolic compounds, and proteins.
31068961	5	13	from	colleters	814:822	arg1	crinipes					860:867	E. crinipes	857:867	E. crinipes	857:867	Due to the low frequency of colleters and lack of visible secretion in E. crinipes, histochemical tests and chemical analyses were not performed for this species.
31068961	0	14	from	Aspects	48:54	arg1	Deep					0:3	Deep	0:3	Deep	0:3	Deep in the Jelly: Histochemical and Functional Aspects of Mucilage-Secreting Floral Colleters in the Orchids Elleanthus brasiliensis and E. crinipes.
31068961	0	14	from	Aspects	48:54	arg1	E.					138:139	E.	138:139	E.	138:139	Deep in the Jelly: Histochemical and Functional Aspects of Mucilage-Secreting Floral Colleters in the Orchids Elleanthus brasiliensis and E. crinipes.
31068961	0	14	from	Aspects	48:54	arg1	Orchids					102:108	Orchids	102:108	Orchids	102:108	Deep in the Jelly: Histochemical and Functional Aspects of Mucilage-Secreting Floral Colleters in the Orchids Elleanthus brasiliensis and E. crinipes.
31068961	8	15	theme	γ-sitosterol	1229:1240	arg1	presence					1217:1224	the presence	1213:1224	the presence of γ-sitosterol and palmitic, linoleic, and stearic acids in the secretion of E. brasiliensis	1213:1318	The GC-MS analysis confirmed the presence of γ-sitosterol and palmitic, linoleic, and stearic acids in the secretion of E. brasiliensis.
31068961	5	16	from	frequency	801:809	arg1	crinipes					860:867	E. crinipes	857:867	E. crinipes	857:867	Due to the low frequency of colleters and lack of visible secretion in E. crinipes, histochemical tests and chemical analyses were not performed for this species.
31068961	10	17	from	occurrence	1412:1421	arg1	orchids					1473:1479	other orchids	1467:1479	other orchids described in the literature	1467:1507	The occurrence of colleters in both species studied and in other orchids described in the literature suggests that these structures are common in the inflorescences of tropical orchids.
31068961	10	17	from	occurrence	1412:1421	arg1	species					1444:1450	both species	1439:1450	both species studied	1439:1458	The occurrence of colleters in both species studied and in other orchids described in the literature suggests that these structures are common in the inflorescences of tropical orchids.
31068961	8	18	theme	palmitic	1246:1253	arg1	acids					1278:1282	palmitic, linoleic, and stearic acids	1246:1282	palmitic, linoleic, and stearic acids	1246:1282	The GC-MS analysis confirmed the presence of γ-sitosterol and palmitic, linoleic, and stearic acids in the secretion of E. brasiliensis.
31068961	8	19	from	presence	1217:1224	arg1	secretion					1291:1299	the secretion	1287:1299	the secretion of E. brasiliensis	1287:1318	The GC-MS analysis confirmed the presence of γ-sitosterol and palmitic, linoleic, and stearic acids in the secretion of E. brasiliensis.
31068961	5	20	theme	chemical	894:901	arg1	analyses					903:910	chemical analyses	894:910	chemical analyses	894:910	Due to the low frequency of colleters and lack of visible secretion in E. crinipes, histochemical tests and chemical analyses were not performed for this species.
31068961	0	21	from	Colleters	85:93	arg1	E.					138:139	E.	138:139	E.	138:139	Deep in the Jelly: Histochemical and Functional Aspects of Mucilage-Secreting Floral Colleters in the Orchids Elleanthus brasiliensis and E. crinipes.
31068961	0	21	from	Colleters	85:93	arg1	Orchids					102:108	Orchids	102:108	Orchids	102:108	Deep in the Jelly: Histochemical and Functional Aspects of Mucilage-Secreting Floral Colleters in the Orchids Elleanthus brasiliensis and E. crinipes.
31068961	8	22	theme	linoleic	1256:1263	arg1	acids					1278:1282	palmitic, linoleic, and stearic acids	1246:1282	palmitic, linoleic, and stearic acids	1246:1282	The GC-MS analysis confirmed the presence of γ-sitosterol and palmitic, linoleic, and stearic acids in the secretion of E. brasiliensis.
31068961	5	23	from	lack	828:831	arg1	crinipes					860:867	E. crinipes	857:867	E. crinipes	857:867	Due to the low frequency of colleters and lack of visible secretion in E. crinipes, histochemical tests and chemical analyses were not performed for this species.
31068961	6	24	theme	brush	968:972	arg1	type					974:977	a brush type	966:977	a brush type	966:977	Colleters are of a brush type and their secretion has, at the same time, hydrophilic and lipophilic components.
31068961	0	25	theme	Mucilage-Secreting	59:76	arg1	Colleters					85:93	Mucilage-Secreting Floral Colleters	59:93	Mucilage-Secreting Floral Colleters in the Orchids Elleanthus brasiliensis and E. crinipes	59:148	Deep in the Jelly: Histochemical and Functional Aspects of Mucilage-Secreting Floral Colleters in the Orchids Elleanthus brasiliensis and E. crinipes.
31068961	5	26	from	crinipes	860:867	arg1	frequency					801:809	the low frequency	793:809	the low frequency of colleters and lack of visible secretion in E. crinipes	793:867	Due to the low frequency of colleters and lack of visible secretion in E. crinipes, histochemical tests and chemical analyses were not performed for this species.
31068961	11	27	from	polysaccharides	1630:1644	arg1	secretion					1653:1661	the secretion	1649:1661	the secretion	1649:1661	In these environments, the hydrated polysaccharides in the secretion form a dense matrix that can act as a physical barrier, and terpenes may help to protect against herbivores and pathogenic microorganisms.
31068961	0	28	from	Deep	0:3	arg1	Aspects					48:54	the Jelly: Histochemical and Functional Aspects	8:54	the Jelly: Histochemical and Functional Aspects of Mucilage-Secreting Floral Colleters in the Orchids Elleanthus brasiliensis and E. crinipes	8:148	Deep in the Jelly: Histochemical and Functional Aspects of Mucilage-Secreting Floral Colleters in the Orchids Elleanthus brasiliensis and E. crinipes.
31068961	7	29	theme	phenolic	1150:1157	arg1	compounds					1159:1167	phenolic compounds	1150:1167	phenolic compounds	1150:1167	Histochemical tests further revealed the presence of pectin, mucilage, lipids, terpenes, phenolic compounds, and proteins.
31068961	0	30	theme	Colleters	85:93	arg1	Aspects					48:54	the Jelly: Histochemical and Functional Aspects	8:54	the Jelly: Histochemical and Functional Aspects of Mucilage-Secreting Floral Colleters in the Orchids Elleanthus brasiliensis and E. crinipes	8:148	Deep in the Jelly: Histochemical and Functional Aspects of Mucilage-Secreting Floral Colleters in the Orchids Elleanthus brasiliensis and E. crinipes.
31068961	12	31	theme	diversity	1876:1884	arg1	knowledge					1832:1840	our knowledge	1828:1840	our knowledge of the morphological and chemical diversity of the secretions produced by orchid colleters	1828:1931	This information broadens our knowledge of the morphological and chemical diversity of the secretions produced by orchid colleters.
31068961	6	32	contain	has	999:1001	arg1	secretion					989:997	their secretion	983:997	their secretion	983:997	Colleters are of a brush type and their secretion has, at the same time, hydrophilic and lipophilic components.
31068961	6	32	contain	has	999:1001	arg2	components					1049:1058	hydrophilic and lipophilic components	1022:1058	hydrophilic and lipophilic components	1022:1058	Colleters are of a brush type and their secretion has, at the same time, hydrophilic and lipophilic components.
31068961	4	33	theme	secretion	710:718	arg1	composition					689:699	the chemical composition	676:699	the chemical composition of their secretion to better understand the functions of these secretory structures	676:783	We aimed to describe the histology of colleters in inflorescences of E. brasiliensis and Elleanthus crinipes, and to analyze the chemical composition of their secretion to better understand the functions of these secretory structures.
31068961	8	34	theme	brasiliensis	1307:1318	arg1	secretion					1291:1299	the secretion	1287:1299	the secretion of E. brasiliensis	1287:1318	The GC-MS analysis confirmed the presence of γ-sitosterol and palmitic, linoleic, and stearic acids in the secretion of E. brasiliensis.
31068961	5	35	theme	low	797:799	arg1	frequency					801:809	the low frequency	793:809	the low frequency of colleters and lack of visible secretion in E. crinipes	793:867	Due to the low frequency of colleters and lack of visible secretion in E. crinipes, histochemical tests and chemical analyses were not performed for this species.
31068961	3	36	theme	Elleanthus	412:421	arg1	brasiliensis					423:434	Elleanthus brasiliensis	412:434	Elleanthus brasiliensis	412:434	Elleanthus brasiliensis is distinguished by the presence of an abundant gelatinous secretion that covers almost all of its inflorescences.
31068961	2	37	theme	them	375:378	arg1	reports					364:370	reports	364:370	reports of them	364:378	Colleters occur in at least 60 families of angiosperms; however, reports of them are scarce for the Orchidaceae.
31068961	2	37	theme	them	375:378	arg1	scarce					384:389	scarce	384:389	scarce	384:389	Colleters occur in at least 60 families of angiosperms; however, reports of them are scarce for the Orchidaceae.
31068961	9	38	theme	polysaccharides	1374:1388	arg1	presence					1362:1369	the possible presence	1349:1369	the possible presence of polysaccharides in the secretion	1349:1405	Infrared analysis indicated the possible presence of polysaccharides in the secretion.
31068961	1	39	theme	phenolic	279:286	arg1	compounds					288:296	phenolic compounds	279:296	phenolic compounds	279:296	Colleters are trichomes or emergencies that produce a sticky exudate consisting of a mixture of mucilage, lipids, terpenes, and phenolic compounds.
31068961	10	40	theme	other	1467:1471	arg1	orchids					1473:1479	other orchids	1467:1479	other orchids described in the literature	1467:1507	The occurrence of colleters in both species studied and in other orchids described in the literature suggests that these structures are common in the inflorescences of tropical orchids.
31068961	4	41	theme	secretory	764:772	arg1	structures					774:783	these secretory structures	758:783	these secretory structures	758:783	We aimed to describe the histology of colleters in inflorescences of E. brasiliensis and Elleanthus crinipes, and to analyze the chemical composition of their secretion to better understand the functions of these secretory structures.
31068961	2	42	theme	angiosperms	342:352	arg1	families					330:337	at least 60 families	318:337	at least 60 families of angiosperms	318:352	Colleters occur in at least 60 families of angiosperms; however, reports of them are scarce for the Orchidaceae.
31068961	6	43	theme	lipophilic	1038:1047	arg1	components					1049:1058	hydrophilic and lipophilic components	1022:1058	hydrophilic and lipophilic components	1022:1058	Colleters are of a brush type and their secretion has, at the same time, hydrophilic and lipophilic components.
31068961	4	44	from	histology	576:584	arg1	inflorescences					602:615	inflorescences	602:615	inflorescences of E. brasiliensis and Elleanthus crinipes	602:658	We aimed to describe the histology of colleters in inflorescences of E. brasiliensis and Elleanthus crinipes, and to analyze the chemical composition of their secretion to better understand the functions of these secretory structures.
31068961	6	45	theme	hydrophilic	1022:1032	arg1	components					1049:1058	hydrophilic and lipophilic components	1022:1058	hydrophilic and lipophilic components	1022:1058	Colleters are of a brush type and their secretion has, at the same time, hydrophilic and lipophilic components.
31068961	10	46	from	inflorescences	1558:1571	arg1	common					1544:1549	common	1544:1549	common	1544:1549	The occurrence of colleters in both species studied and in other orchids described in the literature suggests that these structures are common in the inflorescences of tropical orchids.
31068961	11	47	theme	hydrated	1621:1628	arg1	polysaccharides					1630:1644	the hydrated polysaccharides	1617:1644	the hydrated polysaccharides in the secretion	1617:1661	In these environments, the hydrated polysaccharides in the secretion form a dense matrix that can act as a physical barrier, and terpenes may help to protect against herbivores and pathogenic microorganisms.
31068961	8	48	theme	GC-MS	1188:1192	arg1	analysis					1194:1201	The GC-MS analysis	1184:1201	The GC-MS analysis	1184:1201	The GC-MS analysis confirmed the presence of γ-sitosterol and palmitic, linoleic, and stearic acids in the secretion of E. brasiliensis.
31068961	5	49	theme	lack	828:831	arg1	frequency					801:809	the low frequency	793:809	the low frequency of colleters and lack of visible secretion in E. crinipes	793:867	Due to the low frequency of colleters and lack of visible secretion in E. crinipes, histochemical tests and chemical analyses were not performed for this species.
31068961	4	50	theme	brasiliensis	623:634	arg1	inflorescences					602:615	inflorescences	602:615	inflorescences of E. brasiliensis and Elleanthus crinipes	602:658	We aimed to describe the histology of colleters in inflorescences of E. brasiliensis and Elleanthus crinipes, and to analyze the chemical composition of their secretion to better understand the functions of these secretory structures.
31068961	7	51	theme	compounds	1159:1167	arg1	presence					1102:1109	the presence	1098:1109	the presence of pectin, mucilage, lipids, terpenes, phenolic compounds, and proteins	1098:1181	Histochemical tests further revealed the presence of pectin, mucilage, lipids, terpenes, phenolic compounds, and proteins.
31068961	5	52	theme	visible	836:842	arg1	secretion					844:852	visible secretion	836:852	visible secretion	836:852	Due to the low frequency of colleters and lack of visible secretion in E. crinipes, histochemical tests and chemical analyses were not performed for this species.
31068961	12	53	theme	orchid	1916:1921	arg1	colleters					1923:1931	orchid colleters	1916:1931	orchid colleters	1916:1931	This information broadens our knowledge of the morphological and chemical diversity of the secretions produced by orchid colleters.
31068961	9	54	attach	presence	1362:1369	arg2	polysaccharides					1374:1388	polysaccharides	1374:1388	polysaccharides	1374:1388	Infrared analysis indicated the possible presence of polysaccharides in the secretion.
31068961	9	54	attach	presence	1362:1369	arg1	secretion					1397:1405	the secretion	1393:1405	the secretion	1393:1405	Infrared analysis indicated the possible presence of polysaccharides in the secretion.
31068961	10	55	theme	tropical	1576:1583	arg1	orchids					1585:1591	tropical orchids	1576:1591	tropical orchids	1576:1591	The occurrence of colleters in both species studied and in other orchids described in the literature suggests that these structures are common in the inflorescences of tropical orchids.
31068961	5	56	theme	colleters	814:822	arg1	frequency					801:809	the low frequency	793:809	the low frequency of colleters and lack of visible secretion in E. crinipes	793:867	Due to the low frequency of colleters and lack of visible secretion in E. crinipes, histochemical tests and chemical analyses were not performed for this species.
31068961	5	57	theme	histochemical	870:882	arg1	tests					884:888	histochemical tests	870:888	histochemical tests	870:888	Due to the low frequency of colleters and lack of visible secretion in E. crinipes, histochemical tests and chemical analyses were not performed for this species.
31068961	0	58	from	Orchids	102:108	arg1	Aspects					48:54	the Jelly: Histochemical and Functional Aspects	8:54	the Jelly: Histochemical and Functional Aspects of Mucilage-Secreting Floral Colleters in the Orchids Elleanthus brasiliensis and E. crinipes	8:148	Deep in the Jelly: Histochemical and Functional Aspects of Mucilage-Secreting Floral Colleters in the Orchids Elleanthus brasiliensis and E. crinipes.
31068961	8	59	theme	acids	1278:1282	arg1	presence					1217:1224	the presence	1213:1224	the presence of γ-sitosterol and palmitic, linoleic, and stearic acids in the secretion of E. brasiliensis	1213:1318	The GC-MS analysis confirmed the presence of γ-sitosterol and palmitic, linoleic, and stearic acids in the secretion of E. brasiliensis.
31068961	0	60	theme	Floral	78:83	arg1	Colleters					85:93	Mucilage-Secreting Floral Colleters	59:93	Mucilage-Secreting Floral Colleters in the Orchids Elleanthus brasiliensis and E. crinipes	59:148	Deep in the Jelly: Histochemical and Functional Aspects of Mucilage-Secreting Floral Colleters in the Orchids Elleanthus brasiliensis and E. crinipes.
31068961	12	61	theme	chemical	1867:1874	arg1	diversity					1876:1884	the morphological and chemical diversity	1845:1884	the morphological and chemical diversity of the secretions produced by orchid colleters	1845:1931	This information broadens our knowledge of the morphological and chemical diversity of the secretions produced by orchid colleters.
31068961	7	62	theme	terpenes	1140:1147	arg1	presence					1102:1109	the presence	1098:1109	the presence of pectin, mucilage, lipids, terpenes, phenolic compounds, and proteins	1098:1181	Histochemical tests further revealed the presence of pectin, mucilage, lipids, terpenes, phenolic compounds, and proteins.
31068961	0	63	dep	Orchids	102:108	arg1	the					98:100	the	98:100	the	98:100	Deep in the Jelly: Histochemical and Functional Aspects of Mucilage-Secreting Floral Colleters in the Orchids Elleanthus brasiliensis and E. crinipes.
31068961	0	63	dep	Orchids	102:108	arg1	brasiliensis					121:132	Orchids Elleanthus brasiliensis	102:132	Orchids Elleanthus brasiliensis	102:132	Deep in the Jelly: Histochemical and Functional Aspects of Mucilage-Secreting Floral Colleters in the Orchids Elleanthus brasiliensis and E. crinipes.
31068961	0	63	dep	Orchids	102:108	arg1	crinipes					141:148	crinipes	141:148	crinipes	141:148	Deep in the Jelly: Histochemical and Functional Aspects of Mucilage-Secreting Floral Colleters in the Orchids Elleanthus brasiliensis and E. crinipes.
31068961	8	64	theme	stearic	1270:1276	arg1	acids					1278:1282	palmitic, linoleic, and stearic acids	1246:1282	palmitic, linoleic, and stearic acids	1246:1282	The GC-MS analysis confirmed the presence of γ-sitosterol and palmitic, linoleic, and stearic acids in the secretion of E. brasiliensis.
31068961	11	65	theme	physical	1701:1708	arg1	barrier					1710:1716	a physical barrier	1699:1716	a physical barrier	1699:1716	In these environments, the hydrated polysaccharides in the secretion form a dense matrix that can act as a physical barrier, and terpenes may help to protect against herbivores and pathogenic microorganisms.
31068961	11	65	theme	physical	1701:1708	arg1	matrix					1676:1681	a dense matrix	1668:1681	a dense matrix that can act as a physical barrier, and terpenes may help to protect against herbivores and pathogenic microorganisms	1668:1799	In these environments, the hydrated polysaccharides in the secretion form a dense matrix that can act as a physical barrier, and terpenes may help to protect against herbivores and pathogenic microorganisms.
31068961	7	66	theme	lipids	1132:1137	arg1	presence					1102:1109	the presence	1098:1109	the presence of pectin, mucilage, lipids, terpenes, phenolic compounds, and proteins	1098:1181	Histochemical tests further revealed the presence of pectin, mucilage, lipids, terpenes, phenolic compounds, and proteins.
31068961	7	67	theme	mucilage	1122:1129	arg1	presence					1102:1109	the presence	1098:1109	the presence of pectin, mucilage, lipids, terpenes, phenolic compounds, and proteins	1098:1181	Histochemical tests further revealed the presence of pectin, mucilage, lipids, terpenes, phenolic compounds, and proteins.
31068961	12	68	theme	morphological	1849:1861	arg1	diversity					1876:1884	the morphological and chemical diversity	1845:1884	the morphological and chemical diversity of the secretions produced by orchid colleters	1845:1931	This information broadens our knowledge of the morphological and chemical diversity of the secretions produced by orchid colleters.
31068961	6	69	theme	same	1011:1014	arg1	time					1016:1019	the same time	1007:1019	the same time	1007:1019	Colleters are of a brush type and their secretion has, at the same time, hydrophilic and lipophilic components.
31068961	10	70	theme	orchids	1585:1591	arg1	inflorescences					1558:1571	the inflorescences	1554:1571	the inflorescences of tropical orchids	1554:1591	The occurrence of colleters in both species studied and in other orchids described in the literature suggests that these structures are common in the inflorescences of tropical orchids.
31068961	0	71	theme	Jelly	12:16	arg1	Aspects					48:54	the Jelly: Histochemical and Functional Aspects	8:54	the Jelly: Histochemical and Functional Aspects of Mucilage-Secreting Floral Colleters in the Orchids Elleanthus brasiliensis and E. crinipes	8:148	Deep in the Jelly: Histochemical and Functional Aspects of Mucilage-Secreting Floral Colleters in the Orchids Elleanthus brasiliensis and E. crinipes.
31068961	1	72	theme	terpenes	265:272	arg1	mixture					236:242	a mixture	234:242	a mixture of mucilage, lipids, terpenes, and phenolic compounds	234:296	Colleters are trichomes or emergencies that produce a sticky exudate consisting of a mixture of mucilage, lipids, terpenes, and phenolic compounds.
31068961	9	73	from	presence	1362:1369	arg1	secretion					1397:1405	the secretion	1393:1405	the secretion	1393:1405	Infrared analysis indicated the possible presence of polysaccharides in the secretion.
31068961	3	74	theme	gelatinous	484:493	arg1	secretion					495:503	an abundant gelatinous secretion	472:503	an abundant gelatinous secretion that covers almost all of its inflorescences	472:548	Elleanthus brasiliensis is distinguished by the presence of an abundant gelatinous secretion that covers almost all of its inflorescences.
31068961	0	75	theme	Histochemical	19:31	arg1	Aspects					48:54	the Jelly: Histochemical and Functional Aspects	8:54	the Jelly: Histochemical and Functional Aspects of Mucilage-Secreting Floral Colleters in the Orchids Elleanthus brasiliensis and E. crinipes	8:148	Deep in the Jelly: Histochemical and Functional Aspects of Mucilage-Secreting Floral Colleters in the Orchids Elleanthus brasiliensis and E. crinipes.
31068961	1	76	theme	sticky	205:210	arg1	exudate					212:218	a sticky exudate	203:218	a sticky exudate consisting of a mixture of mucilage, lipids, terpenes, and phenolic compounds	203:296	Colleters are trichomes or emergencies that produce a sticky exudate consisting of a mixture of mucilage, lipids, terpenes, and phenolic compounds.
31068961	10	77	theme	colleters	1426:1434	arg1	occurrence					1412:1421	The occurrence	1408:1421	The occurrence of colleters in both species studied and in other orchids described in the literature	1408:1507	The occurrence of colleters in both species studied and in other orchids described in the literature suggests that these structures are common in the inflorescences of tropical orchids.
31068961	0	78	theme	Functional	37:46	arg1	Aspects					48:54	the Jelly: Histochemical and Functional Aspects	8:54	the Jelly: Histochemical and Functional Aspects of Mucilage-Secreting Floral Colleters in the Orchids Elleanthus brasiliensis and E. crinipes	8:148	Deep in the Jelly: Histochemical and Functional Aspects of Mucilage-Secreting Floral Colleters in the Orchids Elleanthus brasiliensis and E. crinipes.
31068961	1	79	theme	compounds	288:296	arg1	mixture					236:242	a mixture	234:242	a mixture of mucilage, lipids, terpenes, and phenolic compounds	234:296	Colleters are trichomes or emergencies that produce a sticky exudate consisting of a mixture of mucilage, lipids, terpenes, and phenolic compounds.
31068961	11	80	theme	dense	1670:1674	arg1	barrier					1710:1716	a physical barrier	1699:1716	a physical barrier	1699:1716	In these environments, the hydrated polysaccharides in the secretion form a dense matrix that can act as a physical barrier, and terpenes may help to protect against herbivores and pathogenic microorganisms.
31068961	11	80	theme	dense	1670:1674	arg1	matrix					1676:1681	a dense matrix	1668:1681	a dense matrix that can act as a physical barrier, and terpenes may help to protect against herbivores and pathogenic microorganisms	1668:1799	In these environments, the hydrated polysaccharides in the secretion form a dense matrix that can act as a physical barrier, and terpenes may help to protect against herbivores and pathogenic microorganisms.
31068961	0	81	from	E.	138:139	arg1	Aspects					48:54	the Jelly: Histochemical and Functional Aspects	8:54	the Jelly: Histochemical and Functional Aspects of Mucilage-Secreting Floral Colleters in the Orchids Elleanthus brasiliensis and E. crinipes	8:148	Deep in the Jelly: Histochemical and Functional Aspects of Mucilage-Secreting Floral Colleters in the Orchids Elleanthus brasiliensis and E. crinipes.
31068961	11	82	theme	pathogenic	1775:1784	arg1	microorganisms					1786:1799	pathogenic microorganisms	1775:1799	pathogenic microorganisms	1775:1799	In these environments, the hydrated polysaccharides in the secretion form a dense matrix that can act as a physical barrier, and terpenes may help to protect against herbivores and pathogenic microorganisms.
31068961	9	83	theme	Infrared	1321:1328	arg1	analysis					1330:1337	Infrared analysis	1321:1337	Infrared analysis	1321:1337	Infrared analysis indicated the possible presence of polysaccharides in the secretion.
31068961	4	84	theme	colleters	589:597	arg1	histology					576:584	the histology	572:584	the histology of colleters in inflorescences of E. brasiliensis and Elleanthus crinipes	572:658	We aimed to describe the histology of colleters in inflorescences of E. brasiliensis and Elleanthus crinipes, and to analyze the chemical composition of their secretion to better understand the functions of these secretory structures.
31068961	4	85	theme	structures	774:783	arg1	functions					745:753	the functions	741:753	the functions of these secretory structures	741:783	We aimed to describe the histology of colleters in inflorescences of E. brasiliensis and Elleanthus crinipes, and to analyze the chemical composition of their secretion to better understand the functions of these secretory structures.
31068961	3	86	theme	secretion	495:503	arg1	presence					460:467	the presence	456:467	the presence of an abundant gelatinous secretion that covers almost all of its inflorescences	456:548	Elleanthus brasiliensis is distinguished by the presence of an abundant gelatinous secretion that covers almost all of its inflorescences.
30602527	3	0	theme	antifungal	311:320	arg1	tolerance					322:330	the antifungal tolerance	307:330	the antifungal tolerance	307:330	Drug sequestration by the biofilm matrix accounts for much of the antifungal tolerance.
30602527	11	1	theme	biofilm	1278:1284	arg1	matrix					1286:1291	the protective biofilm matrix	1263:1291	the protective biofilm matrix	1263:1291	The conservation of the protective biofilm matrix among Candida spp.
30602527	5	2	theme	triazole	502:509	arg1	triazole					502:509	the available triazole antifungal	488:520	the available triazole antifungal	488:520	We show that matrix sequesters nearly 70% of the available triazole antifungal.
30602527	5	2	theme	triazole	502:509	arg1	%					483:483	nearly 70%	474:483	nearly 70% of the available triazole antifungal	474:520	We show that matrix sequesters nearly 70% of the available triazole antifungal.
30602527	1	3	theme	high	150:153	arg1	mortality					155:163	high mortality	150:163	high mortality	150:163	Candida auris has emerged as an outbreak pathogen associated with high mortality.
30602527	7	4	theme	poor	873:876	arg1	outcomes					886:893	poor patient outcomes	873:893	poor patient outcomes	873:893	This biofilm matrix resistance mechanism appears conserved among Candida species, including C. aurisIMPORTANCECandida auris is an emerging fungal threat linked to poor patient outcomes.
30602527	10	5	theme	antifungal	1201:1210	arg1	therapy					1212:1218	triazole antifungal therapy	1192:1218	triazole antifungal therapy	1192:1218	Our findings reveal one mechanism utilized by C. auris to evade the effect of triazole antifungal therapy during biofilm growth.
30602527	0	6	from	Role	10:13	arg1	Resistance					72:81	Candida auris Drug Resistance	53:81	Candida auris Drug Resistance	53:81	Conserved Role for Biofilm Matrix Polysaccharides in Candida auris Drug Resistance.
30602527	11	7	theme	matrix	1286:1291	arg1	conservation					1247:1258	The conservation	1243:1258	The conservation of the protective biofilm matrix among Candida spp	1243:1309	The conservation of the protective biofilm matrix among Candida spp.
30602527	10	8	theme	triazole	1192:1199	arg1	therapy					1212:1218	triazole antifungal therapy	1192:1218	triazole antifungal therapy	1192:1218	Our findings reveal one mechanism utilized by C. auris to evade the effect of triazole antifungal therapy during biofilm growth.
30602527	7	9	attach	linked	863:868	arg2	threat					856:861	an emerging fungal threat	837:861	an emerging fungal threat linked to poor patient outcomes	837:893	This biofilm matrix resistance mechanism appears conserved among Candida species, including C. aurisIMPORTANCECandida auris is an emerging fungal threat linked to poor patient outcomes.
30602527	7	9	attach	linked	863:868	arg1	outcomes					886:893	poor patient outcomes	873:893	poor patient outcomes	873:893	This biofilm matrix resistance mechanism appears conserved among Candida species, including C. aurisIMPORTANCECandida auris is an emerging fungal threat linked to poor patient outcomes.
30602527	9	10	from	persistence	1052:1062	arg1	patients					1084:1091	patients	1084:1091	patients	1084:1091	Biofilm formation has been suggested as an important factor for persistence of this organism in patients and the environment.
30602527	9	10	from	persistence	1052:1062	arg1	environment					1101:1111	the environment	1097:1111	the environment	1097:1111	Biofilm formation has been suggested as an important factor for persistence of this organism in patients and the environment.
30602527	4	11	dep	composition	378:388	arg1	the					359:361	the	359:361	the	359:361	In this study, we examine the biofilm matrix composition and function for a diverse set of C. auris isolates.
30602527	11	12	theme	protective	1267:1276	arg1	matrix					1286:1291	the protective biofilm matrix	1263:1291	the protective biofilm matrix	1263:1291	The conservation of the protective biofilm matrix among Candida spp.
30602527	9	13	theme	organism	1072:1079	arg1	persistence					1052:1062	persistence	1052:1062	persistence of this organism in patients and the environment	1052:1111	Biofilm formation has been suggested as an important factor for persistence of this organism in patients and the environment.
30602527	7	14	theme	emerging	840:847	arg1	threat					856:861	an emerging fungal threat	837:861	an emerging fungal threat linked to poor patient outcomes	837:893	This biofilm matrix resistance mechanism appears conserved among Candida species, including C. aurisIMPORTANCECandida auris is an emerging fungal threat linked to poor patient outcomes.
30602527	7	15	dep	auris	828:832	arg1	threat					856:861	an emerging fungal threat	837:861	an emerging fungal threat linked to poor patient outcomes	837:893	This biofilm matrix resistance mechanism appears conserved among Candida species, including C. aurisIMPORTANCECandida auris is an emerging fungal threat linked to poor patient outcomes.
30602527	12	16	theme	promising	1331:1339	arg1	target					1373:1378	a promising pan-fungal Candida biofilm drug target	1329:1378	a promising pan-fungal Candida biofilm drug target	1329:1378	suggests that is a promising pan-fungal Candida biofilm drug target.
30602527	7	17	theme	fungal	849:854	arg1	threat					856:861	an emerging fungal threat	837:861	an emerging fungal threat linked to poor patient outcomes	837:893	This biofilm matrix resistance mechanism appears conserved among Candida species, including C. aurisIMPORTANCECandida auris is an emerging fungal threat linked to poor patient outcomes.
30602527	7	18	dep	appears	751:757	arg1	conserved					759:767	conserved	759:767	appears conserved among Candida species, including C. aurisIMPORTANCECandida auris is an emerging fungal threat linked to poor patient outcomes	751:893	This biofilm matrix resistance mechanism appears conserved among Candida species, including C. aurisIMPORTANCECandida auris is an emerging fungal threat linked to poor patient outcomes.
30602527	9	19	theme	Biofilm	988:994	arg1	formation					996:1004	Biofilm formation	988:1004	Biofilm formation	988:1004	Biofilm formation has been suggested as an important factor for persistence of this organism in patients and the environment.
30602527	9	19	theme	Biofilm	988:994	arg1	factor					1041:1046	an important factor	1028:1046	an important factor for persistence of this organism in patients and the environment	1028:1111	Biofilm formation has been suggested as an important factor for persistence of this organism in patients and the environment.
30602527	11	20	theme	Candida	1299:1305	arg1	spp					1307:1309	Candida spp	1299:1309	Candida spp	1299:1309	The conservation of the protective biofilm matrix among Candida spp.
30602527	1	21	theme	Candida	84:90	arg1	auris					92:96	Candida auris	84:96	Candida auris	84:96	Candida auris has emerged as an outbreak pathogen associated with high mortality.
30602527	3	22	theme	Drug	245:248	arg1	sequestration					250:262	Drug sequestration	245:262	Drug sequestration by the biofilm matrix	245:284	Drug sequestration by the biofilm matrix accounts for much of the antifungal tolerance.
30602527	4	23	theme	matrix	371:376	arg1	composition					378:388	biofilm matrix composition	363:388	biofilm matrix composition	363:388	In this study, we examine the biofilm matrix composition and function for a diverse set of C. auris isolates.
30602527	6	24	from	rich	610:613	arg1	polysaccharides					632:646	mannan-glucan polysaccharides	618:646	mannan-glucan polysaccharides	618:646	Like the biofilms formed by other Candida spp., we find that the matrix of C. auris is rich in mannan-glucan polysaccharides and demonstrate that their hydrolysis reduces drug tolerance.
30602527	0	25	theme	Biofilm	19:25	arg1	Polysaccharides					34:48	Biofilm Matrix Polysaccharides	19:48	Biofilm Matrix Polysaccharides	19:48	Conserved Role for Biofilm Matrix Polysaccharides in Candida auris Drug Resistance.
30602527	4	26	theme	biofilm	363:369	arg1	composition					378:388	biofilm matrix composition	363:388	biofilm matrix composition	363:388	In this study, we examine the biofilm matrix composition and function for a diverse set of C. auris isolates.
30602527	6	27	from	polysaccharides	632:646	arg1	rich					610:613	rich	610:613	rich	610:613	Like the biofilms formed by other Candida spp., we find that the matrix of C. auris is rich in mannan-glucan polysaccharides and demonstrate that their hydrolysis reduces drug tolerance.
30602527	6	27	from	polysaccharides	632:646	arg1	matrix					588:593	the matrix	584:593	the matrix of C. auris	584:605	Like the biofilms formed by other Candida spp., we find that the matrix of C. auris is rich in mannan-glucan polysaccharides and demonstrate that their hydrolysis reduces drug tolerance.
30602527	6	28	theme	mannan-glucan	618:630	arg1	polysaccharides					632:646	mannan-glucan polysaccharides	618:646	mannan-glucan polysaccharides	618:646	Like the biofilms formed by other Candida spp., we find that the matrix of C. auris is rich in mannan-glucan polysaccharides and demonstrate that their hydrolysis reduces drug tolerance.
30602527	6	29	theme	Candida	557:563	arg1	spp.					565:568	other Candida spp.	551:568	other Candida spp.	551:568	Like the biofilms formed by other Candida spp., we find that the matrix of C. auris is rich in mannan-glucan polysaccharides and demonstrate that their hydrolysis reduces drug tolerance.
30602527	4	30	theme	auris	427:431	arg1	isolates					433:440	C. auris isolates	424:440	C. auris isolates	424:440	In this study, we examine the biofilm matrix composition and function for a diverse set of C. auris isolates.
30602527	2	31	link	linked	188:193	arg1	resistance					200:209	linked drug resistance	188:209	linked drug resistance	188:209	Biofilm formation and linked drug resistance are common among Candida species.
30602527	6	32	theme	other	551:555	arg1	spp.					565:568	other Candida spp.	551:568	other Candida spp.	551:568	Like the biofilms formed by other Candida spp., we find that the matrix of C. auris is rich in mannan-glucan polysaccharides and demonstrate that their hydrolysis reduces drug tolerance.
30602527	7	33	theme	Candida	775:781	arg1	auris					828:832	auris	828:832	auris	828:832	This biofilm matrix resistance mechanism appears conserved among Candida species, including C. aurisIMPORTANCECandida auris is an emerging fungal threat linked to poor patient outcomes.
30602527	7	33	theme	Candida	775:781	arg1	species					783:789	Candida species	775:789	Candida species	775:789	This biofilm matrix resistance mechanism appears conserved among Candida species, including C. aurisIMPORTANCECandida auris is an emerging fungal threat linked to poor patient outcomes.
30602527	4	34	theme	C.	424:425	arg1	isolates					433:440	C. auris isolates	424:440	C. auris isolates	424:440	In this study, we examine the biofilm matrix composition and function for a diverse set of C. auris isolates.
30602527	12	35	theme	pan-fungal	1341:1350	arg1	target					1373:1378	a promising pan-fungal Candida biofilm drug target	1329:1378	a promising pan-fungal Candida biofilm drug target	1329:1378	suggests that is a promising pan-fungal Candida biofilm drug target.
30602527	8	36	theme	apparent	929:936	arg1	increase					938:945	this apparent increase	924:945	this apparent increase in pathogenicity	924:962	The factors responsible for this apparent increase in pathogenicity remain largely unknown.
30602527	8	37	from	increase	938:945	arg1	pathogenicity					950:962	pathogenicity	950:962	pathogenicity	950:962	The factors responsible for this apparent increase in pathogenicity remain largely unknown.
30602527	0	38	theme	Matrix	27:32	arg1	Polysaccharides					34:48	Biofilm Matrix Polysaccharides	19:48	Biofilm Matrix Polysaccharides	19:48	Conserved Role for Biofilm Matrix Polysaccharides in Candida auris Drug Resistance.
30602527	3	39	theme	biofilm	271:277	arg1	matrix					279:284	the biofilm matrix	267:284	the biofilm matrix	267:284	Drug sequestration by the biofilm matrix accounts for much of the antifungal tolerance.
30602527	2	40	theme	Biofilm	166:172	arg1	formation					174:182	Biofilm formation	166:182	Biofilm formation	166:182	Biofilm formation and linked drug resistance are common among Candida species.
30602527	2	41	theme	Candida	228:234	arg1	species					236:242	Candida species	228:242	Candida species	228:242	Biofilm formation and linked drug resistance are common among Candida species.
30602527	5	42	theme	antifungal	511:520	arg1	triazole					502:509	the available triazole antifungal	488:520	the available triazole antifungal	488:520	We show that matrix sequesters nearly 70% of the available triazole antifungal.
30602527	0	43	theme	Candida	53:59	arg1	Resistance					72:81	Candida auris Drug Resistance	53:81	Candida auris Drug Resistance	53:81	Conserved Role for Biofilm Matrix Polysaccharides in Candida auris Drug Resistance.
30602527	4	44	theme	isolates	433:440	arg1	set					417:419	a diverse set	407:419	a diverse set of C. auris isolates	407:440	In this study, we examine the biofilm matrix composition and function for a diverse set of C. auris isolates.
30602527	4	44	theme	isolates	433:440	arg1	isolates					433:440	C. auris isolates	424:440	C. auris isolates	424:440	In this study, we examine the biofilm matrix composition and function for a diverse set of C. auris isolates.
30602527	12	45	theme	biofilm	1360:1366	arg1	target					1373:1378	a promising pan-fungal Candida biofilm drug target	1329:1378	a promising pan-fungal Candida biofilm drug target	1329:1378	suggests that is a promising pan-fungal Candida biofilm drug target.
30602527	10	46	theme	therapy	1212:1218	arg1	effect					1182:1187	the effect	1178:1187	the effect of triazole antifungal therapy during biofilm growth	1178:1240	Our findings reveal one mechanism utilized by C. auris to evade the effect of triazole antifungal therapy during biofilm growth.
30602527	7	47	theme	resistance	730:739	arg1	mechanism					741:749	This biofilm matrix resistance mechanism	710:749	This biofilm matrix resistance mechanism	710:749	This biofilm matrix resistance mechanism appears conserved among Candida species, including C. aurisIMPORTANCECandida auris is an emerging fungal threat linked to poor patient outcomes.
30602527	4	48	theme	diverse	409:415	arg1	set					417:419	a diverse set	407:419	a diverse set of C. auris isolates	407:440	In this study, we examine the biofilm matrix composition and function for a diverse set of C. auris isolates.
30602527	4	48	theme	diverse	409:415	arg1	isolates					433:440	C. auris isolates	424:440	C. auris isolates	424:440	In this study, we examine the biofilm matrix composition and function for a diverse set of C. auris isolates.
30602527	12	49	theme	Candida	1352:1358	arg1	target					1373:1378	a promising pan-fungal Candida biofilm drug target	1329:1378	a promising pan-fungal Candida biofilm drug target	1329:1378	suggests that is a promising pan-fungal Candida biofilm drug target.
30602527	10	50	theme	biofilm	1227:1233	arg1	growth					1235:1240	biofilm growth	1227:1240	biofilm growth	1227:1240	Our findings reveal one mechanism utilized by C. auris to evade the effect of triazole antifungal therapy during biofilm growth.
30602527	1	51	theme	outbreak	116:123	arg1	pathogen					125:132	an outbreak pathogen	113:132	an outbreak pathogen associated with high mortality	113:163	Candida auris has emerged as an outbreak pathogen associated with high mortality.
30602527	6	52	theme	auris	601:605	arg1	rich					610:613	rich	610:613	rich	610:613	Like the biofilms formed by other Candida spp., we find that the matrix of C. auris is rich in mannan-glucan polysaccharides and demonstrate that their hydrolysis reduces drug tolerance.
30602527	6	52	theme	auris	601:605	arg1	matrix					588:593	the matrix	584:593	the matrix of C. auris	584:605	Like the biofilms formed by other Candida spp., we find that the matrix of C. auris is rich in mannan-glucan polysaccharides and demonstrate that their hydrolysis reduces drug tolerance.
30602527	7	53	theme	biofilm	715:721	arg1	mechanism					741:749	This biofilm matrix resistance mechanism	710:749	This biofilm matrix resistance mechanism	710:749	This biofilm matrix resistance mechanism appears conserved among Candida species, including C. aurisIMPORTANCECandida auris is an emerging fungal threat linked to poor patient outcomes.
30602527	2	54	theme	drug	195:198	arg1	resistance					200:209	linked drug resistance	188:209	linked drug resistance	188:209	Biofilm formation and linked drug resistance are common among Candida species.
30602527	8	55	theme	responsible	908:918	arg1	factors					900:906	The factors	896:906	The factors responsible for this apparent increase in pathogenicity	896:962	The factors responsible for this apparent increase in pathogenicity remain largely unknown.
30602527	12	56	theme	drug	1368:1371	arg1	target					1373:1378	a promising pan-fungal Candida biofilm drug target	1329:1378	a promising pan-fungal Candida biofilm drug target	1329:1378	suggests that is a promising pan-fungal Candida biofilm drug target.
30602527	7	57	theme	patient	878:884	arg1	outcomes					886:893	poor patient outcomes	873:893	poor patient outcomes	873:893	This biofilm matrix resistance mechanism appears conserved among Candida species, including C. aurisIMPORTANCECandida auris is an emerging fungal threat linked to poor patient outcomes.
30602527	7	58	theme	matrix	723:728	arg1	mechanism					741:749	This biofilm matrix resistance mechanism	710:749	This biofilm matrix resistance mechanism	710:749	This biofilm matrix resistance mechanism appears conserved among Candida species, including C. aurisIMPORTANCECandida auris is an emerging fungal threat linked to poor patient outcomes.
30602527	0	59	theme	Drug	67:70	arg1	Resistance					72:81	Candida auris Drug Resistance	53:81	Candida auris Drug Resistance	53:81	Conserved Role for Biofilm Matrix Polysaccharides in Candida auris Drug Resistance.
30602527	2	60	theme	linked	188:193	arg1	resistance					200:209	linked drug resistance	188:209	linked drug resistance	188:209	Biofilm formation and linked drug resistance are common among Candida species.
30602527	6	61	theme	drug	694:697	arg1	tolerance					699:707	drug tolerance	694:707	drug tolerance	694:707	Like the biofilms formed by other Candida spp., we find that the matrix of C. auris is rich in mannan-glucan polysaccharides and demonstrate that their hydrolysis reduces drug tolerance.
30602527	9	62	theme	important	1031:1039	arg1	formation					996:1004	Biofilm formation	988:1004	Biofilm formation	988:1004	Biofilm formation has been suggested as an important factor for persistence of this organism in patients and the environment.
30602527	9	62	theme	important	1031:1039	arg1	factor					1041:1046	an important factor	1028:1046	an important factor for persistence of this organism in patients and the environment	1028:1111	Biofilm formation has been suggested as an important factor for persistence of this organism in patients and the environment.
30602527	0	63	theme	auris	61:65	arg1	Resistance					72:81	Candida auris Drug Resistance	53:81	Candida auris Drug Resistance	53:81	Conserved Role for Biofilm Matrix Polysaccharides in Candida auris Drug Resistance.
30602527	5	64	theme	available	492:500	arg1	triazole					502:509	the available triazole antifungal	488:520	the available triazole antifungal	488:520	We show that matrix sequesters nearly 70% of the available triazole antifungal.
31168539	2	0	theme	LBPS	483:486	arg1	administration					465:478	administration	465:478	administration of LBPS	465:486	The results showed that administration of LBPS could protect immune organs (enhancing immune organ indexes and alleviating immune organ damage), enhance the production of immune-related cytokines (IL-2, IL-6, IL-1β, TNF-α and IFN-γ) and prevent the hepatotoxicity in CTX-induced mice.
31168539	3	1	theme	acids	805:809	arg1	production					773:782	the production	769:782	the production of short-chain fatty acids	769:809	Additionally, LBPS treatment could promote the production of short-chain fatty acids and modulate the composition of the gut microbiota, increasing the relative abundances of Bacteroidaceae, Lactobacillaceae, Prevotellaceae and Verrucomicrobiaceae, which were positively associated with immune traits.
31168539	0	2	from	effects	11:17	arg1	response					87:94	immune response	80:94	immune response	80:94	Modulating effects of polysaccharides from the fruits of Lycium barbarum on the immune response and gut microbiota in cyclophosphamide-treated mice.
31168539	0	2	from	effects	11:17	arg1	microbiota					104:113	gut microbiota	100:113	gut microbiota	100:113	Modulating effects of polysaccharides from the fruits of Lycium barbarum on the immune response and gut microbiota in cyclophosphamide-treated mice.
31168539	0	2	from	effects	11:17	arg1	fruits					47:52	the fruits	43:52	the fruits of Lycium barbarum	43:71	Modulating effects of polysaccharides from the fruits of Lycium barbarum on the immune response and gut microbiota in cyclophosphamide-treated mice.
31168539	4	3	theme	gut	1138:1140	arg1	microbiota					1142:1151	the gut microbiota	1134:1151	the gut microbiota	1134:1151	The present results indicated that LBPS might regulate the immune response depending on the modulation of the gut microbiota, suggesting that LBPS could be developed as special ingredients for immunoregulation in association with the modulation of the gut microbiota.
31168539	1	4	theme	gut	425:427	arg1	microbiota					429:438	the gut microbiota	421:438	the gut microbiota	421:438	In the present study, the effects of Lycium barbarum polysaccharides (LBPS) on immunoregulation and gut microbiota dysbiosis in cyclophosphamide (CTX)-induced mice were investigated to elucidate whether the attenuation of immunosuppression is related to the modulation of the gut microbiota.
31168539	1	5	theme	present	156:162	arg1	study					164:168	the present study	152:168	the present study	152:168	In the present study, the effects of Lycium barbarum polysaccharides (LBPS) on immunoregulation and gut microbiota dysbiosis in cyclophosphamide (CTX)-induced mice were investigated to elucidate whether the attenuation of immunosuppression is related to the modulation of the gut microbiota.
31168539	1	6	theme	microbiota	429:438	arg1	modulation					407:416	the modulation	403:416	the modulation of the gut microbiota	403:438	In the present study, the effects of Lycium barbarum polysaccharides (LBPS) on immunoregulation and gut microbiota dysbiosis in cyclophosphamide (CTX)-induced mice were investigated to elucidate whether the attenuation of immunosuppression is related to the modulation of the gut microbiota.
31168539	3	7	theme	gut	847:849	arg1	microbiota					851:860	the gut microbiota	843:860	the gut microbiota	843:860	Additionally, LBPS treatment could promote the production of short-chain fatty acids and modulate the composition of the gut microbiota, increasing the relative abundances of Bacteroidaceae, Lactobacillaceae, Prevotellaceae and Verrucomicrobiaceae, which were positively associated with immune traits.
31168539	1	8	theme	gut	249:251	arg1	dysbiosis					264:272	gut microbiota dysbiosis	249:272	gut microbiota dysbiosis	249:272	In the present study, the effects of Lycium barbarum polysaccharides (LBPS) on immunoregulation and gut microbiota dysbiosis in cyclophosphamide (CTX)-induced mice were investigated to elucidate whether the attenuation of immunosuppression is related to the modulation of the gut microbiota.
31168539	0	9	theme	immune	80:85	arg1	response					87:94	immune response	80:94	immune response	80:94	Modulating effects of polysaccharides from the fruits of Lycium barbarum on the immune response and gut microbiota in cyclophosphamide-treated mice.
31168539	3	10	theme	relative	878:885	arg1	abundances					887:896	the relative abundances	874:896	the relative abundances of Bacteroidaceae, Lactobacillaceae, Prevotellaceae and Verrucomicrobiaceae, which were positively associated with immune traits	874:1025	Additionally, LBPS treatment could promote the production of short-chain fatty acids and modulate the composition of the gut microbiota, increasing the relative abundances of Bacteroidaceae, Lactobacillaceae, Prevotellaceae and Verrucomicrobiaceae, which were positively associated with immune traits.
31168539	1	11	theme	microbiota	253:262	arg1	dysbiosis					264:272	gut microbiota dysbiosis	249:272	gut microbiota dysbiosis	249:272	In the present study, the effects of Lycium barbarum polysaccharides (LBPS) on immunoregulation and gut microbiota dysbiosis in cyclophosphamide (CTX)-induced mice were investigated to elucidate whether the attenuation of immunosuppression is related to the modulation of the gut microbiota.
31168539	0	12	theme	gut	100:102	arg1	microbiota					104:113	gut microbiota	100:113	gut microbiota	100:113	Modulating effects of polysaccharides from the fruits of Lycium barbarum on the immune response and gut microbiota in cyclophosphamide-treated mice.
31168539	0	13	from	fruits	47:52	arg1	polysaccharides					22:36	polysaccharides	22:36	polysaccharides from the fruits of Lycium barbarum	22:71	Modulating effects of polysaccharides from the fruits of Lycium barbarum on the immune response and gut microbiota in cyclophosphamide-treated mice.
31168539	0	13	from	fruits	47:52	arg1	effects					11:17	effects	11:17	effects of polysaccharides from the fruits of Lycium barbarum on the immune response and gut microbiota in cyclophosphamide-treated mice	11:146	Modulating effects of polysaccharides from the fruits of Lycium barbarum on the immune response and gut microbiota in cyclophosphamide-treated mice.
31168539	2	14	theme	cytokines	627:635	arg1	production					598:607	the production	594:607	the production of immune-related cytokines (IL-2, IL-6, IL-1β, TNF-α and IFN-γ)	594:672	The results showed that administration of LBPS could protect immune organs (enhancing immune organ indexes and alleviating immune organ damage), enhance the production of immune-related cytokines (IL-2, IL-6, IL-1β, TNF-α and IFN-γ) and prevent the hepatotoxicity in CTX-induced mice.
31168539	0	15	dep	response	87:94	arg1	the					76:78	the	76:78	the	76:78	Modulating effects of polysaccharides from the fruits of Lycium barbarum on the immune response and gut microbiota in cyclophosphamide-treated mice.
31168539	2	16	theme	immune	502:507	arg1	organs					509:514	immune organs	502:514	immune organs (enhancing immune organ indexes and alleviating immune organ damage)	502:583	The results showed that administration of LBPS could protect immune organs (enhancing immune organ indexes and alleviating immune organ damage), enhance the production of immune-related cytokines (IL-2, IL-6, IL-1β, TNF-α and IFN-γ) and prevent the hepatotoxicity in CTX-induced mice.
31168539	2	17	theme	immune-related	612:625	arg1	IL-2					638:641	IL-2	638:641	IL-2	638:641	The results showed that administration of LBPS could protect immune organs (enhancing immune organ indexes and alleviating immune organ damage), enhance the production of immune-related cytokines (IL-2, IL-6, IL-1β, TNF-α and IFN-γ) and prevent the hepatotoxicity in CTX-induced mice.
31168539	2	17	theme	immune-related	612:625	arg1	IL-6					644:647	IL-6	644:647	IL-6	644:647	The results showed that administration of LBPS could protect immune organs (enhancing immune organ indexes and alleviating immune organ damage), enhance the production of immune-related cytokines (IL-2, IL-6, IL-1β, TNF-α and IFN-γ) and prevent the hepatotoxicity in CTX-induced mice.
31168539	2	17	theme	immune-related	612:625	arg1	IFN-γ					667:671	IFN-γ	667:671	IFN-γ	667:671	The results showed that administration of LBPS could protect immune organs (enhancing immune organ indexes and alleviating immune organ damage), enhance the production of immune-related cytokines (IL-2, IL-6, IL-1β, TNF-α and IFN-γ) and prevent the hepatotoxicity in CTX-induced mice.
31168539	2	17	theme	immune-related	612:625	arg1	cytokines					627:635	immune-related cytokines	612:635	immune-related cytokines (IL-2, IL-6, IL-1β, TNF-α and IFN-γ)	612:672	The results showed that administration of LBPS could protect immune organs (enhancing immune organ indexes and alleviating immune organ damage), enhance the production of immune-related cytokines (IL-2, IL-6, IL-1β, TNF-α and IFN-γ) and prevent the hepatotoxicity in CTX-induced mice.
31168539	2	17	theme	immune-related	612:625	arg1	IL-1β					650:654	IL-1β	650:654	IL-1β	650:654	The results showed that administration of LBPS could protect immune organs (enhancing immune organ indexes and alleviating immune organ damage), enhance the production of immune-related cytokines (IL-2, IL-6, IL-1β, TNF-α and IFN-γ) and prevent the hepatotoxicity in CTX-induced mice.
31168539	2	17	theme	immune-related	612:625	arg1	TNF-α					657:661	TNF-α	657:661	TNF-α	657:661	The results showed that administration of LBPS could protect immune organs (enhancing immune organ indexes and alleviating immune organ damage), enhance the production of immune-related cytokines (IL-2, IL-6, IL-1β, TNF-α and IFN-γ) and prevent the hepatotoxicity in CTX-induced mice.
31168539	4	18	theme	microbiota	1142:1151	arg1	modulation					1120:1129	the modulation	1116:1129	the modulation of the gut microbiota	1116:1151	The present results indicated that LBPS might regulate the immune response depending on the modulation of the gut microbiota, suggesting that LBPS could be developed as special ingredients for immunoregulation in association with the modulation of the gut microbiota.
31168539	1	19	from	dysbiosis	264:272	arg1	mice					308:311	cyclophosphamide (CTX)-induced mice	277:311	cyclophosphamide (CTX)-induced mice	277:311	In the present study, the effects of Lycium barbarum polysaccharides (LBPS) on immunoregulation and gut microbiota dysbiosis in cyclophosphamide (CTX)-induced mice were investigated to elucidate whether the attenuation of immunosuppression is related to the modulation of the gut microbiota.
31168539	4	20	theme	special	1197:1203	arg1	LBPS					1170:1173	LBPS	1170:1173	LBPS	1170:1173	The present results indicated that LBPS might regulate the immune response depending on the modulation of the gut microbiota, suggesting that LBPS could be developed as special ingredients for immunoregulation in association with the modulation of the gut microbiota.
31168539	4	20	theme	special	1197:1203	arg1	ingredients					1205:1215	special ingredients	1197:1215	special ingredients for immunoregulation in association with the modulation of the gut microbiota	1197:1293	The present results indicated that LBPS might regulate the immune response depending on the modulation of the gut microbiota, suggesting that LBPS could be developed as special ingredients for immunoregulation in association with the modulation of the gut microbiota.
31168539	4	21	from	immunoregulation	1221:1236	arg1	association					1241:1251	association	1241:1251	association with the modulation of the gut microbiota	1241:1293	The present results indicated that LBPS might regulate the immune response depending on the modulation of the gut microbiota, suggesting that LBPS could be developed as special ingredients for immunoregulation in association with the modulation of the gut microbiota.
31168539	2	22	theme	CTX-induced	708:718	arg1	mice					720:723	CTX-induced mice	708:723	CTX-induced mice	708:723	The results showed that administration of LBPS could protect immune organs (enhancing immune organ indexes and alleviating immune organ damage), enhance the production of immune-related cytokines (IL-2, IL-6, IL-1β, TNF-α and IFN-γ) and prevent the hepatotoxicity in CTX-induced mice.
31168539	2	23	theme	organ	534:538	arg1	indexes					540:546	immune organ indexes	527:546	immune organ indexes	527:546	The results showed that administration of LBPS could protect immune organs (enhancing immune organ indexes and alleviating immune organ damage), enhance the production of immune-related cytokines (IL-2, IL-6, IL-1β, TNF-α and IFN-γ) and prevent the hepatotoxicity in CTX-induced mice.
31168539	0	24	theme	polysaccharides	22:36	arg1	effects					11:17	effects	11:17	effects of polysaccharides from the fruits of Lycium barbarum on the immune response and gut microbiota in cyclophosphamide-treated mice	11:146	Modulating effects of polysaccharides from the fruits of Lycium barbarum on the immune response and gut microbiota in cyclophosphamide-treated mice.
31168539	2	25	dep	organs	509:514	arg1	alleviating					552:562	alleviating	552:562	alleviating immune organ damage	552:582	The results showed that administration of LBPS could protect immune organs (enhancing immune organ indexes and alleviating immune organ damage), enhance the production of immune-related cytokines (IL-2, IL-6, IL-1β, TNF-α and IFN-γ) and prevent the hepatotoxicity in CTX-induced mice.
31168539	2	25	dep	organs	509:514	arg1	enhancing					517:525	enhancing	517:525	enhancing immune organ indexes	517:546	The results showed that administration of LBPS could protect immune organs (enhancing immune organ indexes and alleviating immune organ damage), enhance the production of immune-related cytokines (IL-2, IL-6, IL-1β, TNF-α and IFN-γ) and prevent the hepatotoxicity in CTX-induced mice.
31168539	3	26	theme	fatty	799:803	arg1	acids					805:809	short-chain fatty acids	787:809	short-chain fatty acids	787:809	Additionally, LBPS treatment could promote the production of short-chain fatty acids and modulate the composition of the gut microbiota, increasing the relative abundances of Bacteroidaceae, Lactobacillaceae, Prevotellaceae and Verrucomicrobiaceae, which were positively associated with immune traits.
31168539	3	27	theme	Lactobacillaceae	917:932	arg1	abundances					887:896	the relative abundances	874:896	the relative abundances of Bacteroidaceae, Lactobacillaceae, Prevotellaceae and Verrucomicrobiaceae, which were positively associated with immune traits	874:1025	Additionally, LBPS treatment could promote the production of short-chain fatty acids and modulate the composition of the gut microbiota, increasing the relative abundances of Bacteroidaceae, Lactobacillaceae, Prevotellaceae and Verrucomicrobiaceae, which were positively associated with immune traits.
31168539	4	28	theme	present	1032:1038	arg1	results					1040:1046	The present results	1028:1046	The present results	1028:1046	The present results indicated that LBPS might regulate the immune response depending on the modulation of the gut microbiota, suggesting that LBPS could be developed as special ingredients for immunoregulation in association with the modulation of the gut microbiota.
31168539	1	29	theme	immunosuppression	371:387	arg1	related					392:398	related	392:398	related	392:398	In the present study, the effects of Lycium barbarum polysaccharides (LBPS) on immunoregulation and gut microbiota dysbiosis in cyclophosphamide (CTX)-induced mice were investigated to elucidate whether the attenuation of immunosuppression is related to the modulation of the gut microbiota.
31168539	1	29	theme	immunosuppression	371:387	arg1	attenuation					356:366	the attenuation	352:366	the attenuation of immunosuppression	352:387	In the present study, the effects of Lycium barbarum polysaccharides (LBPS) on immunoregulation and gut microbiota dysbiosis in cyclophosphamide (CTX)-induced mice were investigated to elucidate whether the attenuation of immunosuppression is related to the modulation of the gut microbiota.
31168539	1	30	theme	Lycium	186:191	arg1	LBPS					219:222	LBPS	219:222	LBPS	219:222	In the present study, the effects of Lycium barbarum polysaccharides (LBPS) on immunoregulation and gut microbiota dysbiosis in cyclophosphamide (CTX)-induced mice were investigated to elucidate whether the attenuation of immunosuppression is related to the modulation of the gut microbiota.
31168539	1	30	theme	Lycium	186:191	arg1	polysaccharides					202:216	Lycium barbarum polysaccharides	186:216	Lycium barbarum polysaccharides (LBPS)	186:223	In the present study, the effects of Lycium barbarum polysaccharides (LBPS) on immunoregulation and gut microbiota dysbiosis in cyclophosphamide (CTX)-induced mice were investigated to elucidate whether the attenuation of immunosuppression is related to the modulation of the gut microbiota.
31168539	1	31	from	immunoregulation	228:243	arg1	mice					308:311	cyclophosphamide (CTX)-induced mice	277:311	cyclophosphamide (CTX)-induced mice	277:311	In the present study, the effects of Lycium barbarum polysaccharides (LBPS) on immunoregulation and gut microbiota dysbiosis in cyclophosphamide (CTX)-induced mice were investigated to elucidate whether the attenuation of immunosuppression is related to the modulation of the gut microbiota.
31168539	4	32	theme	microbiota	1284:1293	arg1	modulation					1262:1271	the modulation	1258:1271	the modulation of the gut microbiota	1258:1293	The present results indicated that LBPS might regulate the immune response depending on the modulation of the gut microbiota, suggesting that LBPS could be developed as special ingredients for immunoregulation in association with the modulation of the gut microbiota.
31168539	1	33	theme	barbarum	193:200	arg1	LBPS					219:222	LBPS	219:222	LBPS	219:222	In the present study, the effects of Lycium barbarum polysaccharides (LBPS) on immunoregulation and gut microbiota dysbiosis in cyclophosphamide (CTX)-induced mice were investigated to elucidate whether the attenuation of immunosuppression is related to the modulation of the gut microbiota.
31168539	1	33	theme	barbarum	193:200	arg1	polysaccharides					202:216	Lycium barbarum polysaccharides	186:216	Lycium barbarum polysaccharides (LBPS)	186:223	In the present study, the effects of Lycium barbarum polysaccharides (LBPS) on immunoregulation and gut microbiota dysbiosis in cyclophosphamide (CTX)-induced mice were investigated to elucidate whether the attenuation of immunosuppression is related to the modulation of the gut microbiota.
31168539	4	34	theme	gut	1280:1282	arg1	microbiota					1284:1293	the gut microbiota	1276:1293	the gut microbiota	1276:1293	The present results indicated that LBPS might regulate the immune response depending on the modulation of the gut microbiota, suggesting that LBPS could be developed as special ingredients for immunoregulation in association with the modulation of the gut microbiota.
31168539	0	35	theme	cyclophosphamide-treated	118:141	arg1	mice					143:146	cyclophosphamide-treated mice	118:146	cyclophosphamide-treated mice	118:146	Modulating effects of polysaccharides from the fruits of Lycium barbarum on the immune response and gut microbiota in cyclophosphamide-treated mice.
31168539	2	36	dep	cytokines	627:635	arg1	IL-2					638:641	IL-2	638:641	IL-2	638:641	The results showed that administration of LBPS could protect immune organs (enhancing immune organ indexes and alleviating immune organ damage), enhance the production of immune-related cytokines (IL-2, IL-6, IL-1β, TNF-α and IFN-γ) and prevent the hepatotoxicity in CTX-induced mice.
31168539	2	36	dep	cytokines	627:635	arg1	IL-6					644:647	IL-6	644:647	IL-6	644:647	The results showed that administration of LBPS could protect immune organs (enhancing immune organ indexes and alleviating immune organ damage), enhance the production of immune-related cytokines (IL-2, IL-6, IL-1β, TNF-α and IFN-γ) and prevent the hepatotoxicity in CTX-induced mice.
31168539	2	36	dep	cytokines	627:635	arg1	IFN-γ					667:671	IFN-γ	667:671	IFN-γ	667:671	The results showed that administration of LBPS could protect immune organs (enhancing immune organ indexes and alleviating immune organ damage), enhance the production of immune-related cytokines (IL-2, IL-6, IL-1β, TNF-α and IFN-γ) and prevent the hepatotoxicity in CTX-induced mice.
31168539	2	36	dep	cytokines	627:635	arg1	cytokines					627:635	immune-related cytokines	612:635	immune-related cytokines (IL-2, IL-6, IL-1β, TNF-α and IFN-γ)	612:672	The results showed that administration of LBPS could protect immune organs (enhancing immune organ indexes and alleviating immune organ damage), enhance the production of immune-related cytokines (IL-2, IL-6, IL-1β, TNF-α and IFN-γ) and prevent the hepatotoxicity in CTX-induced mice.
31168539	2	36	dep	cytokines	627:635	arg1	IL-1β					650:654	IL-1β	650:654	IL-1β	650:654	The results showed that administration of LBPS could protect immune organs (enhancing immune organ indexes and alleviating immune organ damage), enhance the production of immune-related cytokines (IL-2, IL-6, IL-1β, TNF-α and IFN-γ) and prevent the hepatotoxicity in CTX-induced mice.
31168539	2	36	dep	cytokines	627:635	arg1	TNF-α					657:661	TNF-α	657:661	TNF-α	657:661	The results showed that administration of LBPS could protect immune organs (enhancing immune organ indexes and alleviating immune organ damage), enhance the production of immune-related cytokines (IL-2, IL-6, IL-1β, TNF-α and IFN-γ) and prevent the hepatotoxicity in CTX-induced mice.
31168539	1	37	theme	polysaccharides	202:216	arg1	effects					175:181	the effects	171:181	the effects of Lycium barbarum polysaccharides (LBPS) on immunoregulation and gut microbiota dysbiosis in cyclophosphamide (CTX)-induced mice	171:311	In the present study, the effects of Lycium barbarum polysaccharides (LBPS) on immunoregulation and gut microbiota dysbiosis in cyclophosphamide (CTX)-induced mice were investigated to elucidate whether the attenuation of immunosuppression is related to the modulation of the gut microbiota.
31168539	4	38	with	association	1241:1251	arg1	modulation					1262:1271	the modulation	1258:1271	the modulation of the gut microbiota	1258:1293	The present results indicated that LBPS might regulate the immune response depending on the modulation of the gut microbiota, suggesting that LBPS could be developed as special ingredients for immunoregulation in association with the modulation of the gut microbiota.
31168539	3	39	theme	Verrucomicrobiaceae	954:972	arg1	abundances					887:896	the relative abundances	874:896	the relative abundances of Bacteroidaceae, Lactobacillaceae, Prevotellaceae and Verrucomicrobiaceae, which were positively associated with immune traits	874:1025	Additionally, LBPS treatment could promote the production of short-chain fatty acids and modulate the composition of the gut microbiota, increasing the relative abundances of Bacteroidaceae, Lactobacillaceae, Prevotellaceae and Verrucomicrobiaceae, which were positively associated with immune traits.
31168539	3	40	theme	immune	1013:1018	arg1	traits					1020:1025	immune traits	1013:1025	immune traits	1013:1025	Additionally, LBPS treatment could promote the production of short-chain fatty acids and modulate the composition of the gut microbiota, increasing the relative abundances of Bacteroidaceae, Lactobacillaceae, Prevotellaceae and Verrucomicrobiaceae, which were positively associated with immune traits.
31168539	2	41	theme	immune	527:532	arg1	indexes					540:546	immune organ indexes	527:546	immune organ indexes	527:546	The results showed that administration of LBPS could protect immune organs (enhancing immune organ indexes and alleviating immune organ damage), enhance the production of immune-related cytokines (IL-2, IL-6, IL-1β, TNF-α and IFN-γ) and prevent the hepatotoxicity in CTX-induced mice.
31168539	1	42	theme	-induced	299:306	arg1	mice					308:311	cyclophosphamide (CTX)-induced mice	277:311	cyclophosphamide (CTX)-induced mice	277:311	In the present study, the effects of Lycium barbarum polysaccharides (LBPS) on immunoregulation and gut microbiota dysbiosis in cyclophosphamide (CTX)-induced mice were investigated to elucidate whether the attenuation of immunosuppression is related to the modulation of the gut microbiota.
31168539	2	43	theme	organ	571:575	arg1	damage					577:582	immune organ damage	564:582	immune organ damage	564:582	The results showed that administration of LBPS could protect immune organs (enhancing immune organ indexes and alleviating immune organ damage), enhance the production of immune-related cytokines (IL-2, IL-6, IL-1β, TNF-α and IFN-γ) and prevent the hepatotoxicity in CTX-induced mice.
31168539	3	44	theme	LBPS	740:743	arg1	treatment					745:753	LBPS treatment	740:753	LBPS treatment	740:753	Additionally, LBPS treatment could promote the production of short-chain fatty acids and modulate the composition of the gut microbiota, increasing the relative abundances of Bacteroidaceae, Lactobacillaceae, Prevotellaceae and Verrucomicrobiaceae, which were positively associated with immune traits.
31168539	2	45	theme	immune	564:569	arg1	damage					577:582	immune organ damage	564:582	immune organ damage	564:582	The results showed that administration of LBPS could protect immune organs (enhancing immune organ indexes and alleviating immune organ damage), enhance the production of immune-related cytokines (IL-2, IL-6, IL-1β, TNF-α and IFN-γ) and prevent the hepatotoxicity in CTX-induced mice.
31168539	3	46	theme	Prevotellaceae	935:948	arg1	abundances					887:896	the relative abundances	874:896	the relative abundances of Bacteroidaceae, Lactobacillaceae, Prevotellaceae and Verrucomicrobiaceae, which were positively associated with immune traits	874:1025	Additionally, LBPS treatment could promote the production of short-chain fatty acids and modulate the composition of the gut microbiota, increasing the relative abundances of Bacteroidaceae, Lactobacillaceae, Prevotellaceae and Verrucomicrobiaceae, which were positively associated with immune traits.
31168539	0	47	from	response	87:94	arg1	mice					143:146	cyclophosphamide-treated mice	118:146	cyclophosphamide-treated mice	118:146	Modulating effects of polysaccharides from the fruits of Lycium barbarum on the immune response and gut microbiota in cyclophosphamide-treated mice.
31168539	0	48	theme	barbarum	64:71	arg1	fruits					47:52	the fruits	43:52	the fruits of Lycium barbarum	43:71	Modulating effects of polysaccharides from the fruits of Lycium barbarum on the immune response and gut microbiota in cyclophosphamide-treated mice.
31168539	2	49	from	hepatotoxicity	690:703	arg1	mice					720:723	CTX-induced mice	708:723	CTX-induced mice	708:723	The results showed that administration of LBPS could protect immune organs (enhancing immune organ indexes and alleviating immune organ damage), enhance the production of immune-related cytokines (IL-2, IL-6, IL-1β, TNF-α and IFN-γ) and prevent the hepatotoxicity in CTX-induced mice.
31168539	1	50	from	effects	175:181	arg1	dysbiosis					264:272	gut microbiota dysbiosis	249:272	gut microbiota dysbiosis	249:272	In the present study, the effects of Lycium barbarum polysaccharides (LBPS) on immunoregulation and gut microbiota dysbiosis in cyclophosphamide (CTX)-induced mice were investigated to elucidate whether the attenuation of immunosuppression is related to the modulation of the gut microbiota.
31168539	1	50	from	effects	175:181	arg1	immunoregulation					228:243	immunoregulation	228:243	immunoregulation	228:243	In the present study, the effects of Lycium barbarum polysaccharides (LBPS) on immunoregulation and gut microbiota dysbiosis in cyclophosphamide (CTX)-induced mice were investigated to elucidate whether the attenuation of immunosuppression is related to the modulation of the gut microbiota.
31168539	4	51	theme	immune	1087:1092	arg1	response					1094:1101	the immune response	1083:1101	the immune response	1083:1101	The present results indicated that LBPS might regulate the immune response depending on the modulation of the gut microbiota, suggesting that LBPS could be developed as special ingredients for immunoregulation in association with the modulation of the gut microbiota.
31168539	3	52	theme	short-chain	787:797	arg1	acids					805:809	short-chain fatty acids	787:809	short-chain fatty acids	787:809	Additionally, LBPS treatment could promote the production of short-chain fatty acids and modulate the composition of the gut microbiota, increasing the relative abundances of Bacteroidaceae, Lactobacillaceae, Prevotellaceae and Verrucomicrobiaceae, which were positively associated with immune traits.
31168539	3	53	theme	microbiota	851:860	arg1	composition					828:838	the composition	824:838	the composition of the gut microbiota	824:860	Additionally, LBPS treatment could promote the production of short-chain fatty acids and modulate the composition of the gut microbiota, increasing the relative abundances of Bacteroidaceae, Lactobacillaceae, Prevotellaceae and Verrucomicrobiaceae, which were positively associated with immune traits.
31168539	0	54	from	microbiota	104:113	arg1	mice					143:146	cyclophosphamide-treated mice	118:146	cyclophosphamide-treated mice	118:146	Modulating effects of polysaccharides from the fruits of Lycium barbarum on the immune response and gut microbiota in cyclophosphamide-treated mice.
30861043	7	0	theme	breast	1817:1822	arg1	cancer					1824:1829	breast cancer	1817:1829	breast cancer	1817:1829	Preliminary results demonstrated that Raman spectroscopy and Super Paramagnetic Clustering method can be used to discriminate between breast cancer, leukemia and cervical cancer samples using blood serum samples.
30861043	6	1	with	agreement	1619:1627	arg1	results					1634:1640	results	1634:1640	results of Super Paramagnetic Clustering method	1634:1680	Finally, as a cross check method, the standard method to classify Raman spectra of breast cancer, leukemia and cervical cancer, known as principal components analysis, was used showing excellent agreement with results of Super Paramagnetic Clustering method.
30861043	6	2	theme	cross	1438:1442	arg1	method					1450:1455	a cross check method	1436:1455	a cross check method	1436:1455	Finally, as a cross check method, the standard method to classify Raman spectra of breast cancer, leukemia and cervical cancer, known as principal components analysis, was used showing excellent agreement with results of Super Paramagnetic Clustering method.
30861043	6	2	theme	cross	1438:1442	arg1	method					1471:1476	the standard method	1458:1476	the standard method to classify Raman spectra of breast cancer, leukemia and cervical cancer	1458:1549	Finally, as a cross check method, the standard method to classify Raman spectra of breast cancer, leukemia and cervical cancer, known as principal components analysis, was used showing excellent agreement with results of Super Paramagnetic Clustering method.
30861043	7	3	used	used	1788:1791	arg2	spectroscopy					1727:1738	Raman spectroscopy	1721:1738	Raman spectroscopy	1721:1738	Preliminary results demonstrated that Raman spectroscopy and Super Paramagnetic Clustering method can be used to discriminate between breast cancer, leukemia and cervical cancer samples using blood serum samples.
30861043	7	3	used	used	1788:1791	arg2	method					1774:1779	Super Paramagnetic Clustering method	1744:1779	Super Paramagnetic Clustering method	1744:1779	Preliminary results demonstrated that Raman spectroscopy and Super Paramagnetic Clustering method can be used to discriminate between breast cancer, leukemia and cervical cancer samples using blood serum samples.
30861043	6	4	theme	breast	1507:1512	arg1	cancer					1514:1519	breast cancer	1507:1519	breast cancer	1507:1519	Finally, as a cross check method, the standard method to classify Raman spectra of breast cancer, leukemia and cervical cancer, known as principal components analysis, was used showing excellent agreement with results of Super Paramagnetic Clustering method.
30861043	4	5	theme	bulged	1114:1119	arg1	1040					1129:1132	1040	1129:1132	1040	1129:1132	According to these spectra, the band associated with amide I (1654 cm-1) and one of two shoulders assigned to amide III (1230-1282 cm-1) allowed discriminating leukemia from breast and cervical cancer, whereas band 714 cm-1 (polysaccharides) achieves to differentiate cervical cancer from leukemia and breast cancer, and bulged region, 1040 - 1100 cm-1 (phenylalanine, phospholipid) discriminated breast cancer from leukemia and cervical cancer.
30861043	4	5	theme	bulged	1114:1119	arg1	cm-1					1141:1144	1100 cm-1	1136:1144	1100 cm-1 (phenylalanine, phospholipid)	1136:1174	According to these spectra, the band associated with amide I (1654 cm-1) and one of two shoulders assigned to amide III (1230-1282 cm-1) allowed discriminating leukemia from breast and cervical cancer, whereas band 714 cm-1 (polysaccharides) achieves to differentiate cervical cancer from leukemia and breast cancer, and bulged region, 1040 - 1100 cm-1 (phenylalanine, phospholipid) discriminated breast cancer from leukemia and cervical cancer.
30861043	4	5	theme	bulged	1114:1119	arg1	region					1121:1126	bulged region	1114:1126	bulged region	1114:1126	According to these spectra, the band associated with amide I (1654 cm-1) and one of two shoulders assigned to amide III (1230-1282 cm-1) allowed discriminating leukemia from breast and cervical cancer, whereas band 714 cm-1 (polysaccharides) achieves to differentiate cervical cancer from leukemia and breast cancer, and bulged region, 1040 - 1100 cm-1 (phenylalanine, phospholipid) discriminated breast cancer from leukemia and cervical cancer.
30861043	5	6	theme	serum	1409:1413	arg1	samples					1415:1421	serum samples	1409:1421	serum samples	1409:1421	Subsequently, Super Paramagnetic Clustering method was applied to Raman spectra to study similarity relationships between cancers based on the biochemical composition of serum samples.
30861043	7	7	theme	Clustering	1763:1772	arg1	method					1774:1779	Super Paramagnetic Clustering method	1744:1779	Super Paramagnetic Clustering method	1744:1779	Preliminary results demonstrated that Raman spectroscopy and Super Paramagnetic Clustering method can be used to discriminate between breast cancer, leukemia and cervical cancer samples using blood serum samples.
30861043	3	8	theme	Raman	586:590	arg1	spectra					592:598	firstly average Raman spectra	570:598	firstly average Raman spectra of the three cancers	570:619	Comparing firstly average Raman spectra of the three cancers, some peaks that allowed differentiating one cancer from other were identified, however, other peaks allowed concluding that there are biochemical similarities among them.
30861043	6	9	theme	method	1675:1680	arg1	results					1634:1640	results	1634:1640	results of Super Paramagnetic Clustering method	1634:1680	Finally, as a cross check method, the standard method to classify Raman spectra of breast cancer, leukemia and cervical cancer, known as principal components analysis, was used showing excellent agreement with results of Super Paramagnetic Clustering method.
30861043	4	10	theme	breast	1095:1100	arg1	cancer					1102:1107	breast cancer	1095:1107	breast cancer	1095:1107	According to these spectra, the band associated with amide I (1654 cm-1) and one of two shoulders assigned to amide III (1230-1282 cm-1) allowed discriminating leukemia from breast and cervical cancer, whereas band 714 cm-1 (polysaccharides) achieves to differentiate cervical cancer from leukemia and breast cancer, and bulged region, 1040 - 1100 cm-1 (phenylalanine, phospholipid) discriminated breast cancer from leukemia and cervical cancer.
30861043	0	11	theme	cancer	28:33	arg1	types					35:39	different cancer types	18:39	different cancer types	18:39	Discrimination of different cancer types clustering Raman spectra by a super paramagnetic stochastic network approach.
30861043	6	12	theme	principal	1561:1569	arg1	analysis					1582:1589	principal components analysis	1561:1589	principal components analysis	1561:1589	Finally, as a cross check method, the standard method to classify Raman spectra of breast cancer, leukemia and cervical cancer, known as principal components analysis, was used showing excellent agreement with results of Super Paramagnetic Clustering method.
30861043	4	13	from	leukemia	1082:1089	arg1	cancer					1070:1075	cervical cancer	1061:1075	cervical cancer from leukemia and breast cancer, and bulged region, 1040 - 1100 cm-1 (phenylalanine, phospholipid) discriminated breast cancer from leukemia and cervical cancer	1061:1236	According to these spectra, the band associated with amide I (1654 cm-1) and one of two shoulders assigned to amide III (1230-1282 cm-1) allowed discriminating leukemia from breast and cervical cancer, whereas band 714 cm-1 (polysaccharides) achieves to differentiate cervical cancer from leukemia and breast cancer, and bulged region, 1040 - 1100 cm-1 (phenylalanine, phospholipid) discriminated breast cancer from leukemia and cervical cancer.
30861043	4	14	theme	shoulders	881:889	arg1	one					870:872	one	870:872	one	870:872	According to these spectra, the band associated with amide I (1654 cm-1) and one of two shoulders assigned to amide III (1230-1282 cm-1) allowed discriminating leukemia from breast and cervical cancer, whereas band 714 cm-1 (polysaccharides) achieves to differentiate cervical cancer from leukemia and breast cancer, and bulged region, 1040 - 1100 cm-1 (phenylalanine, phospholipid) discriminated breast cancer from leukemia and cervical cancer.
30861043	4	14	theme	shoulders	881:889	arg1	shoulders					881:889	two shoulders	877:889	two shoulders assigned to amide III (1230-1282 cm-1)	877:928	According to these spectra, the band associated with amide I (1654 cm-1) and one of two shoulders assigned to amide III (1230-1282 cm-1) allowed discriminating leukemia from breast and cervical cancer, whereas band 714 cm-1 (polysaccharides) achieves to differentiate cervical cancer from leukemia and breast cancer, and bulged region, 1040 - 1100 cm-1 (phenylalanine, phospholipid) discriminated breast cancer from leukemia and cervical cancer.
30861043	6	15	theme	Paramagnetic	1651:1662	arg1	method					1675:1680	Super Paramagnetic Clustering method	1645:1680	Super Paramagnetic Clustering method	1645:1680	Finally, as a cross check method, the standard method to classify Raman spectra of breast cancer, leukemia and cervical cancer, known as principal components analysis, was used showing excellent agreement with results of Super Paramagnetic Clustering method.
30861043	5	16	theme	Super	1253:1257	arg1	method					1283:1288	Super Paramagnetic Clustering method	1253:1288	Super Paramagnetic Clustering method	1253:1288	Subsequently, Super Paramagnetic Clustering method was applied to Raman spectra to study similarity relationships between cancers based on the biochemical composition of serum samples.
30861043	6	17	theme	cancer	1544:1549	arg1	spectra					1496:1502	Raman spectra	1490:1502	Raman spectra of breast cancer, leukemia and cervical cancer	1490:1549	Finally, as a cross check method, the standard method to classify Raman spectra of breast cancer, leukemia and cervical cancer, known as principal components analysis, was used showing excellent agreement with results of Super Paramagnetic Clustering method.
30861043	7	18	theme	Raman	1721:1725	arg1	spectroscopy					1727:1738	Raman spectroscopy	1721:1738	Raman spectroscopy	1721:1738	Preliminary results demonstrated that Raman spectroscopy and Super Paramagnetic Clustering method can be used to discriminate between breast cancer, leukemia and cervical cancer samples using blood serum samples.
30861043	1	19	theme	cancer	199:204	arg1	detection					182:190	detection	182:190	detection of the cancer	182:204	Based in high sensitivity and specificity reported recently in detection of the cancer, the technique of Raman spectroscopy is proposed to discriminate between breast cancer, leukemia and cervical cancer using blood serum samples from patients officially diagnosed.
30861043	5	20	theme	Clustering	1272:1281	arg1	method					1283:1288	Super Paramagnetic Clustering method	1253:1288	Super Paramagnetic Clustering method	1253:1288	Subsequently, Super Paramagnetic Clustering method was applied to Raman spectra to study similarity relationships between cancers based on the biochemical composition of serum samples.
30861043	1	21	theme	blood	329:333	arg1	samples					341:347	blood serum samples	329:347	blood serum samples from patients officially diagnosed	329:382	Based in high sensitivity and specificity reported recently in detection of the cancer, the technique of Raman spectroscopy is proposed to discriminate between breast cancer, leukemia and cervical cancer using blood serum samples from patients officially diagnosed.
30861043	2	22	theme	physics	499:505	arg1	concepts					507:514	statistical physics concepts	487:514	statistical physics concepts with a stochastic approach	487:541	In order to classify Raman spectra, clustering method known as Super Paramagnetic Clustering based on statistical physics concepts with a stochastic approach was implemented.
30861043	0	23	theme	super	71:75	arg1	approach					109:116	a super paramagnetic stochastic network approach	69:116	a super paramagnetic stochastic network approach	69:116	Discrimination of different cancer types clustering Raman spectra by a super paramagnetic stochastic network approach.
30861043	7	24	theme	Preliminary	1683:1693	arg1	results					1695:1701	Preliminary results	1683:1701	Preliminary results	1683:1701	Preliminary results demonstrated that Raman spectroscopy and Super Paramagnetic Clustering method can be used to discriminate between breast cancer, leukemia and cervical cancer samples using blood serum samples.
30861043	0	25	theme	stochastic	90:99	arg1	approach					109:116	a super paramagnetic stochastic network approach	69:116	a super paramagnetic stochastic network approach	69:116	Discrimination of different cancer types clustering Raman spectra by a super paramagnetic stochastic network approach.
30861043	2	26	theme	stochastic	523:532	arg1	approach					534:541	a stochastic approach	521:541	a stochastic approach	521:541	In order to classify Raman spectra, clustering method known as Super Paramagnetic Clustering based on statistical physics concepts with a stochastic approach was implemented.
30861043	1	27	theme	diagnosed	374:382	arg1	patients					354:361	patients	354:361	patients officially diagnosed	354:382	Based in high sensitivity and specificity reported recently in detection of the cancer, the technique of Raman spectroscopy is proposed to discriminate between breast cancer, leukemia and cervical cancer using blood serum samples from patients officially diagnosed.
30861043	2	28	theme	Super	448:452	arg1	Clustering					467:476	Super Paramagnetic Clustering	448:476	Super Paramagnetic Clustering based on statistical physics concepts with a stochastic approach	448:541	In order to classify Raman spectra, clustering method known as Super Paramagnetic Clustering based on statistical physics concepts with a stochastic approach was implemented.
30861043	1	29	theme	breast	279:284	arg1	cancer					286:291	breast cancer	279:291	breast cancer	279:291	Based in high sensitivity and specificity reported recently in detection of the cancer, the technique of Raman spectroscopy is proposed to discriminate between breast cancer, leukemia and cervical cancer using blood serum samples from patients officially diagnosed.
30861043	2	30	theme	clustering	421:430	arg1	method					432:437	clustering method	421:437	clustering method known as Super Paramagnetic Clustering based on statistical physics concepts with a stochastic approach	421:541	In order to classify Raman spectra, clustering method known as Super Paramagnetic Clustering based on statistical physics concepts with a stochastic approach was implemented.
30861043	0	31	theme	different	18:26	arg1	types					35:39	different cancer types	18:39	different cancer types	18:39	Discrimination of different cancer types clustering Raman spectra by a super paramagnetic stochastic network approach.
30861043	0	32	theme	types	35:39	arg1	Discrimination					0:13	Discrimination	0:13	Discrimination of different cancer types	0:39	Discrimination of different cancer types clustering Raman spectra by a super paramagnetic stochastic network approach.
30861043	7	33	theme	cervical	1845:1852	arg1	cancer					1854:1859	cervical cancer	1845:1859	cervical cancer	1845:1859	Preliminary results demonstrated that Raman spectroscopy and Super Paramagnetic Clustering method can be used to discriminate between breast cancer, leukemia and cervical cancer samples using blood serum samples.
30861043	4	34	theme	amide	846:850	arg1	I					852:852	amide I	846:852	amide I (1654 cm-1)	846:864	According to these spectra, the band associated with amide I (1654 cm-1) and one of two shoulders assigned to amide III (1230-1282 cm-1) allowed discriminating leukemia from breast and cervical cancer, whereas band 714 cm-1 (polysaccharides) achieves to differentiate cervical cancer from leukemia and breast cancer, and bulged region, 1040 - 1100 cm-1 (phenylalanine, phospholipid) discriminated breast cancer from leukemia and cervical cancer.
30861043	4	34	theme	amide	846:850	arg1	cm-1					860:863	1654 cm-1	855:863	1654 cm-1	855:863	According to these spectra, the band associated with amide I (1654 cm-1) and one of two shoulders assigned to amide III (1230-1282 cm-1) allowed discriminating leukemia from breast and cervical cancer, whereas band 714 cm-1 (polysaccharides) achieves to differentiate cervical cancer from leukemia and breast cancer, and bulged region, 1040 - 1100 cm-1 (phenylalanine, phospholipid) discriminated breast cancer from leukemia and cervical cancer.
30861043	0	35	theme	Raman	52:56	arg1	spectra					58:64	Raman spectra	52:64	Raman spectra	52:64	Discrimination of different cancer types clustering Raman spectra by a super paramagnetic stochastic network approach.
30861043	4	36	from	cancer	987:992	arg1	leukemia					953:960	discriminating leukemia	938:960	discriminating leukemia from breast and cervical cancer	938:992	According to these spectra, the band associated with amide I (1654 cm-1) and one of two shoulders assigned to amide III (1230-1282 cm-1) allowed discriminating leukemia from breast and cervical cancer, whereas band 714 cm-1 (polysaccharides) achieves to differentiate cervical cancer from leukemia and breast cancer, and bulged region, 1040 - 1100 cm-1 (phenylalanine, phospholipid) discriminated breast cancer from leukemia and cervical cancer.
30861043	7	37	theme	serum	1881:1885	arg1	samples					1887:1893	blood serum samples	1875:1893	blood serum samples	1875:1893	Preliminary results demonstrated that Raman spectroscopy and Super Paramagnetic Clustering method can be used to discriminate between breast cancer, leukemia and cervical cancer samples using blood serum samples.
30861043	7	38	theme	leukemia	1832:1839	arg1	samples					1861:1867	breast cancer, leukemia and cervical cancer samples	1817:1867	breast cancer, leukemia and cervical cancer samples using blood serum samples	1817:1893	Preliminary results demonstrated that Raman spectroscopy and Super Paramagnetic Clustering method can be used to discriminate between breast cancer, leukemia and cervical cancer samples using blood serum samples.
30861043	4	39	theme	cervical	1061:1068	arg1	cancer					1070:1075	cervical cancer	1061:1075	cervical cancer from leukemia and breast cancer, and bulged region, 1040 - 1100 cm-1 (phenylalanine, phospholipid) discriminated breast cancer from leukemia and cervical cancer	1061:1236	According to these spectra, the band associated with amide I (1654 cm-1) and one of two shoulders assigned to amide III (1230-1282 cm-1) allowed discriminating leukemia from breast and cervical cancer, whereas band 714 cm-1 (polysaccharides) achieves to differentiate cervical cancer from leukemia and breast cancer, and bulged region, 1040 - 1100 cm-1 (phenylalanine, phospholipid) discriminated breast cancer from leukemia and cervical cancer.
30861043	4	40	theme	cervical	1222:1229	arg1	cancer					1231:1236	cervical cancer	1222:1236	cervical cancer	1222:1236	According to these spectra, the band associated with amide I (1654 cm-1) and one of two shoulders assigned to amide III (1230-1282 cm-1) allowed discriminating leukemia from breast and cervical cancer, whereas band 714 cm-1 (polysaccharides) achieves to differentiate cervical cancer from leukemia and breast cancer, and bulged region, 1040 - 1100 cm-1 (phenylalanine, phospholipid) discriminated breast cancer from leukemia and cervical cancer.
30861043	5	41	theme	biochemical	1382:1392	arg1	composition					1394:1404	the biochemical composition	1378:1404	the biochemical composition of serum samples	1378:1421	Subsequently, Super Paramagnetic Clustering method was applied to Raman spectra to study similarity relationships between cancers based on the biochemical composition of serum samples.
30861043	6	42	theme	standard	1462:1469	arg1	method					1450:1455	a cross check method	1436:1455	a cross check method	1436:1455	Finally, as a cross check method, the standard method to classify Raman spectra of breast cancer, leukemia and cervical cancer, known as principal components analysis, was used showing excellent agreement with results of Super Paramagnetic Clustering method.
30861043	6	42	theme	standard	1462:1469	arg1	method					1471:1476	the standard method	1458:1476	the standard method to classify Raman spectra of breast cancer, leukemia and cervical cancer	1458:1549	Finally, as a cross check method, the standard method to classify Raman spectra of breast cancer, leukemia and cervical cancer, known as principal components analysis, was used showing excellent agreement with results of Super Paramagnetic Clustering method.
30861043	7	43	theme	cancer	1824:1829	arg1	samples					1861:1867	breast cancer, leukemia and cervical cancer samples	1817:1867	breast cancer, leukemia and cervical cancer samples using blood serum samples	1817:1893	Preliminary results demonstrated that Raman spectroscopy and Super Paramagnetic Clustering method can be used to discriminate between breast cancer, leukemia and cervical cancer samples using blood serum samples.
30861043	1	44	theme	spectroscopy	230:241	arg1	technique					211:219	the technique	207:219	the technique of Raman spectroscopy	207:241	Based in high sensitivity and specificity reported recently in detection of the cancer, the technique of Raman spectroscopy is proposed to discriminate between breast cancer, leukemia and cervical cancer using blood serum samples from patients officially diagnosed.
30861043	6	45	dep	method	1471:1476	arg1	classify					1481:1488	classify	1481:1488	to classify Raman spectra of breast cancer, leukemia and cervical cancer	1478:1549	Finally, as a cross check method, the standard method to classify Raman spectra of breast cancer, leukemia and cervical cancer, known as principal components analysis, was used showing excellent agreement with results of Super Paramagnetic Clustering method.
30861043	6	46	theme	check	1444:1448	arg1	method					1450:1455	a cross check method	1436:1455	a cross check method	1436:1455	Finally, as a cross check method, the standard method to classify Raman spectra of breast cancer, leukemia and cervical cancer, known as principal components analysis, was used showing excellent agreement with results of Super Paramagnetic Clustering method.
30861043	6	46	theme	check	1444:1448	arg1	method					1471:1476	the standard method	1458:1476	the standard method to classify Raman spectra of breast cancer, leukemia and cervical cancer	1458:1549	Finally, as a cross check method, the standard method to classify Raman spectra of breast cancer, leukemia and cervical cancer, known as principal components analysis, was used showing excellent agreement with results of Super Paramagnetic Clustering method.
30861043	4	47	dep	cancer	1197:1202	arg1	leukemia					1209:1216	leukemia	1209:1216	leukemia	1209:1216	According to these spectra, the band associated with amide I (1654 cm-1) and one of two shoulders assigned to amide III (1230-1282 cm-1) allowed discriminating leukemia from breast and cervical cancer, whereas band 714 cm-1 (polysaccharides) achieves to differentiate cervical cancer from leukemia and breast cancer, and bulged region, 1040 - 1100 cm-1 (phenylalanine, phospholipid) discriminated breast cancer from leukemia and cervical cancer.
30861043	4	47	dep	cancer	1197:1202	arg1	cancer					1231:1236	cervical cancer	1222:1236	cervical cancer	1222:1236	According to these spectra, the band associated with amide I (1654 cm-1) and one of two shoulders assigned to amide III (1230-1282 cm-1) allowed discriminating leukemia from breast and cervical cancer, whereas band 714 cm-1 (polysaccharides) achieves to differentiate cervical cancer from leukemia and breast cancer, and bulged region, 1040 - 1100 cm-1 (phenylalanine, phospholipid) discriminated breast cancer from leukemia and cervical cancer.
30861043	6	48	theme	cancer	1514:1519	arg1	spectra					1496:1502	Raman spectra	1490:1502	Raman spectra of breast cancer, leukemia and cervical cancer	1490:1549	Finally, as a cross check method, the standard method to classify Raman spectra of breast cancer, leukemia and cervical cancer, known as principal components analysis, was used showing excellent agreement with results of Super Paramagnetic Clustering method.
30861043	4	49	from	region	1121:1126	arg1	cancer					1070:1075	cervical cancer	1061:1075	cervical cancer from leukemia and breast cancer, and bulged region, 1040 - 1100 cm-1 (phenylalanine, phospholipid) discriminated breast cancer from leukemia and cervical cancer	1061:1236	According to these spectra, the band associated with amide I (1654 cm-1) and one of two shoulders assigned to amide III (1230-1282 cm-1) allowed discriminating leukemia from breast and cervical cancer, whereas band 714 cm-1 (polysaccharides) achieves to differentiate cervical cancer from leukemia and breast cancer, and bulged region, 1040 - 1100 cm-1 (phenylalanine, phospholipid) discriminated breast cancer from leukemia and cervical cancer.
30861043	7	50	theme	Paramagnetic	1750:1761	arg1	method					1774:1779	Super Paramagnetic Clustering method	1744:1779	Super Paramagnetic Clustering method	1744:1779	Preliminary results demonstrated that Raman spectroscopy and Super Paramagnetic Clustering method can be used to discriminate between breast cancer, leukemia and cervical cancer samples using blood serum samples.
30861043	5	51	theme	samples	1415:1421	arg1	composition					1394:1404	the biochemical composition	1378:1404	the biochemical composition of serum samples	1378:1421	Subsequently, Super Paramagnetic Clustering method was applied to Raman spectra to study similarity relationships between cancers based on the biochemical composition of serum samples.
30861043	3	52	theme	average	578:584	arg1	spectra					592:598	firstly average Raman spectra	570:598	firstly average Raman spectra of the three cancers	570:619	Comparing firstly average Raman spectra of the three cancers, some peaks that allowed differentiating one cancer from other were identified, however, other peaks allowed concluding that there are biochemical similarities among them.
30861043	4	53	dep	phenylalanine	1147:1159	arg1	phospholipid					1162:1173	phospholipid	1162:1173	phospholipid	1162:1173	According to these spectra, the band associated with amide I (1654 cm-1) and one of two shoulders assigned to amide III (1230-1282 cm-1) allowed discriminating leukemia from breast and cervical cancer, whereas band 714 cm-1 (polysaccharides) achieves to differentiate cervical cancer from leukemia and breast cancer, and bulged region, 1040 - 1100 cm-1 (phenylalanine, phospholipid) discriminated breast cancer from leukemia and cervical cancer.
30861043	4	54	from	cancer	1102:1107	arg1	cancer					1070:1075	cervical cancer	1061:1075	cervical cancer from leukemia and breast cancer, and bulged region, 1040 - 1100 cm-1 (phenylalanine, phospholipid) discriminated breast cancer from leukemia and cervical cancer	1061:1236	According to these spectra, the band associated with amide I (1654 cm-1) and one of two shoulders assigned to amide III (1230-1282 cm-1) allowed discriminating leukemia from breast and cervical cancer, whereas band 714 cm-1 (polysaccharides) achieves to differentiate cervical cancer from leukemia and breast cancer, and bulged region, 1040 - 1100 cm-1 (phenylalanine, phospholipid) discriminated breast cancer from leukemia and cervical cancer.
30861043	3	55	theme	biochemical	756:766	arg1	similarities					768:779	biochemical similarities	756:779	biochemical similarities among them	756:790	Comparing firstly average Raman spectra of the three cancers, some peaks that allowed differentiating one cancer from other were identified, however, other peaks allowed concluding that there are biochemical similarities among them.
30861043	3	56	theme	cancers	613:619	arg1	spectra					592:598	firstly average Raman spectra	570:598	firstly average Raman spectra of the three cancers	570:619	Comparing firstly average Raman spectra of the three cancers, some peaks that allowed differentiating one cancer from other were identified, however, other peaks allowed concluding that there are biochemical similarities among them.
30861043	6	57	theme	Clustering	1664:1673	arg1	method					1675:1680	Super Paramagnetic Clustering method	1645:1680	Super Paramagnetic Clustering method	1645:1680	Finally, as a cross check method, the standard method to classify Raman spectra of breast cancer, leukemia and cervical cancer, known as principal components analysis, was used showing excellent agreement with results of Super Paramagnetic Clustering method.
30861043	6	58	theme	Super	1645:1649	arg1	method					1675:1680	Super Paramagnetic Clustering method	1645:1680	Super Paramagnetic Clustering method	1645:1680	Finally, as a cross check method, the standard method to classify Raman spectra of breast cancer, leukemia and cervical cancer, known as principal components analysis, was used showing excellent agreement with results of Super Paramagnetic Clustering method.
30861043	1	59	theme	high	128:131	arg1	sensitivity					133:143	high sensitivity	128:143	high sensitivity	128:143	Based in high sensitivity and specificity reported recently in detection of the cancer, the technique of Raman spectroscopy is proposed to discriminate between breast cancer, leukemia and cervical cancer using blood serum samples from patients officially diagnosed.
30861043	7	60	theme	Super	1744:1748	arg1	method					1774:1779	Super Paramagnetic Clustering method	1744:1779	Super Paramagnetic Clustering method	1744:1779	Preliminary results demonstrated that Raman spectroscopy and Super Paramagnetic Clustering method can be used to discriminate between breast cancer, leukemia and cervical cancer samples using blood serum samples.
30861043	6	61	theme	Raman	1490:1494	arg1	spectra					1496:1502	Raman spectra	1490:1502	Raman spectra of breast cancer, leukemia and cervical cancer	1490:1549	Finally, as a cross check method, the standard method to classify Raman spectra of breast cancer, leukemia and cervical cancer, known as principal components analysis, was used showing excellent agreement with results of Super Paramagnetic Clustering method.
30861043	5	62	theme	Paramagnetic	1259:1270	arg1	method					1283:1288	Super Paramagnetic Clustering method	1253:1288	Super Paramagnetic Clustering method	1253:1288	Subsequently, Super Paramagnetic Clustering method was applied to Raman spectra to study similarity relationships between cancers based on the biochemical composition of serum samples.
30861043	6	63	theme	cervical	1535:1542	arg1	cancer					1544:1549	cervical cancer	1535:1549	cervical cancer	1535:1549	Finally, as a cross check method, the standard method to classify Raman spectra of breast cancer, leukemia and cervical cancer, known as principal components analysis, was used showing excellent agreement with results of Super Paramagnetic Clustering method.
30861043	0	64	theme	paramagnetic	77:88	arg1	approach					109:116	a super paramagnetic stochastic network approach	69:116	a super paramagnetic stochastic network approach	69:116	Discrimination of different cancer types clustering Raman spectra by a super paramagnetic stochastic network approach.
30861043	1	65	theme	serum	335:339	arg1	samples					341:347	blood serum samples	329:347	blood serum samples from patients officially diagnosed	329:382	Based in high sensitivity and specificity reported recently in detection of the cancer, the technique of Raman spectroscopy is proposed to discriminate between breast cancer, leukemia and cervical cancer using blood serum samples from patients officially diagnosed.
30861043	6	66	theme	leukemia	1522:1529	arg1	spectra					1496:1502	Raman spectra	1490:1502	Raman spectra of breast cancer, leukemia and cervical cancer	1490:1549	Finally, as a cross check method, the standard method to classify Raman spectra of breast cancer, leukemia and cervical cancer, known as principal components analysis, was used showing excellent agreement with results of Super Paramagnetic Clustering method.
30861043	2	67	theme	statistical	487:497	arg1	concepts					507:514	statistical physics concepts	487:514	statistical physics concepts with a stochastic approach	487:541	In order to classify Raman spectra, clustering method known as Super Paramagnetic Clustering based on statistical physics concepts with a stochastic approach was implemented.
30861043	0	68	theme	network	101:107	arg1	approach					109:116	a super paramagnetic stochastic network approach	69:116	a super paramagnetic stochastic network approach	69:116	Discrimination of different cancer types clustering Raman spectra by a super paramagnetic stochastic network approach.
30861043	6	69	theme	excellent	1609:1617	arg1	agreement					1619:1627	excellent agreement	1609:1627	excellent agreement with results of Super Paramagnetic Clustering method	1609:1680	Finally, as a cross check method, the standard method to classify Raman spectra of breast cancer, leukemia and cervical cancer, known as principal components analysis, was used showing excellent agreement with results of Super Paramagnetic Clustering method.
30861043	5	70	theme	Raman	1305:1309	arg1	spectra					1311:1317	Raman spectra	1305:1317	Raman spectra	1305:1317	Subsequently, Super Paramagnetic Clustering method was applied to Raman spectra to study similarity relationships between cancers based on the biochemical composition of serum samples.
30861043	2	71	with	concepts	507:514	arg1	approach					534:541	a stochastic approach	521:541	a stochastic approach	521:541	In order to classify Raman spectra, clustering method known as Super Paramagnetic Clustering based on statistical physics concepts with a stochastic approach was implemented.
30861043	1	72	theme	Raman	224:228	arg1	spectroscopy					230:241	Raman spectroscopy	224:241	Raman spectroscopy	224:241	Based in high sensitivity and specificity reported recently in detection of the cancer, the technique of Raman spectroscopy is proposed to discriminate between breast cancer, leukemia and cervical cancer using blood serum samples from patients officially diagnosed.
30861043	4	73	theme	discriminating	938:951	arg1	leukemia					953:960	discriminating leukemia	938:960	discriminating leukemia from breast and cervical cancer	938:992	According to these spectra, the band associated with amide I (1654 cm-1) and one of two shoulders assigned to amide III (1230-1282 cm-1) allowed discriminating leukemia from breast and cervical cancer, whereas band 714 cm-1 (polysaccharides) achieves to differentiate cervical cancer from leukemia and breast cancer, and bulged region, 1040 - 1100 cm-1 (phenylalanine, phospholipid) discriminated breast cancer from leukemia and cervical cancer.
30861043	4	74	theme	band	1003:1006	arg1	polysaccharides					1018:1032	polysaccharides	1018:1032	polysaccharides	1018:1032	According to these spectra, the band associated with amide I (1654 cm-1) and one of two shoulders assigned to amide III (1230-1282 cm-1) allowed discriminating leukemia from breast and cervical cancer, whereas band 714 cm-1 (polysaccharides) achieves to differentiate cervical cancer from leukemia and breast cancer, and bulged region, 1040 - 1100 cm-1 (phenylalanine, phospholipid) discriminated breast cancer from leukemia and cervical cancer.
30861043	4	74	theme	band	1003:1006	arg1	cm-1					1012:1015	band 714 cm-1	1003:1015	band 714 cm-1 (polysaccharides)	1003:1033	According to these spectra, the band associated with amide I (1654 cm-1) and one of two shoulders assigned to amide III (1230-1282 cm-1) allowed discriminating leukemia from breast and cervical cancer, whereas band 714 cm-1 (polysaccharides) achieves to differentiate cervical cancer from leukemia and breast cancer, and bulged region, 1040 - 1100 cm-1 (phenylalanine, phospholipid) discriminated breast cancer from leukemia and cervical cancer.
30861043	4	75	theme	breast	1190:1195	arg1	cancer					1197:1202	breast cancer	1190:1202	breast cancer	1190:1202	According to these spectra, the band associated with amide I (1654 cm-1) and one of two shoulders assigned to amide III (1230-1282 cm-1) allowed discriminating leukemia from breast and cervical cancer, whereas band 714 cm-1 (polysaccharides) achieves to differentiate cervical cancer from leukemia and breast cancer, and bulged region, 1040 - 1100 cm-1 (phenylalanine, phospholipid) discriminated breast cancer from leukemia and cervical cancer.
30861043	1	76	from	patients	354:361	arg1	samples					341:347	blood serum samples	329:347	blood serum samples from patients officially diagnosed	329:382	Based in high sensitivity and specificity reported recently in detection of the cancer, the technique of Raman spectroscopy is proposed to discriminate between breast cancer, leukemia and cervical cancer using blood serum samples from patients officially diagnosed.
30861043	6	77	theme	components	1571:1580	arg1	analysis					1582:1589	principal components analysis	1561:1589	principal components analysis	1561:1589	Finally, as a cross check method, the standard method to classify Raman spectra of breast cancer, leukemia and cervical cancer, known as principal components analysis, was used showing excellent agreement with results of Super Paramagnetic Clustering method.
30861043	6	78	used	used	1596:1599	arg2	method					1471:1476	the standard method	1458:1476	the standard method to classify Raman spectra of breast cancer, leukemia and cervical cancer	1458:1549	Finally, as a cross check method, the standard method to classify Raman spectra of breast cancer, leukemia and cervical cancer, known as principal components analysis, was used showing excellent agreement with results of Super Paramagnetic Clustering method.
30861043	6	78	used	used	1596:1599	arg2	method					1450:1455	a cross check method	1436:1455	a cross check method	1436:1455	Finally, as a cross check method, the standard method to classify Raman spectra of breast cancer, leukemia and cervical cancer, known as principal components analysis, was used showing excellent agreement with results of Super Paramagnetic Clustering method.
30861043	4	79	theme	cervical	978:985	arg1	cancer					987:992	cervical cancer	978:992	cervical cancer	978:992	According to these spectra, the band associated with amide I (1654 cm-1) and one of two shoulders assigned to amide III (1230-1282 cm-1) allowed discriminating leukemia from breast and cervical cancer, whereas band 714 cm-1 (polysaccharides) achieves to differentiate cervical cancer from leukemia and breast cancer, and bulged region, 1040 - 1100 cm-1 (phenylalanine, phospholipid) discriminated breast cancer from leukemia and cervical cancer.
30861043	2	80	theme	Raman	406:410	arg1	spectra					412:418	Raman spectra	406:418	Raman spectra	406:418	In order to classify Raman spectra, clustering method known as Super Paramagnetic Clustering based on statistical physics concepts with a stochastic approach was implemented.
30861043	1	81	theme	cervical	307:314	arg1	cancer					316:321	cervical cancer	307:321	cervical cancer	307:321	Based in high sensitivity and specificity reported recently in detection of the cancer, the technique of Raman spectroscopy is proposed to discriminate between breast cancer, leukemia and cervical cancer using blood serum samples from patients officially diagnosed.
30861043	7	82	theme	blood	1875:1879	arg1	samples					1887:1893	blood serum samples	1875:1893	blood serum samples	1875:1893	Preliminary results demonstrated that Raman spectroscopy and Super Paramagnetic Clustering method can be used to discriminate between breast cancer, leukemia and cervical cancer samples using blood serum samples.
30861043	7	83	theme	cancer	1854:1859	arg1	samples					1861:1867	breast cancer, leukemia and cervical cancer samples	1817:1867	breast cancer, leukemia and cervical cancer samples using blood serum samples	1817:1893	Preliminary results demonstrated that Raman spectroscopy and Super Paramagnetic Clustering method can be used to discriminate between breast cancer, leukemia and cervical cancer samples using blood serum samples.
30861043	2	84	theme	Paramagnetic	454:465	arg1	Clustering					467:476	Super Paramagnetic Clustering	448:476	Super Paramagnetic Clustering based on statistical physics concepts with a stochastic approach	448:541	In order to classify Raman spectra, clustering method known as Super Paramagnetic Clustering based on statistical physics concepts with a stochastic approach was implemented.
30861043	5	85	theme	similarity	1328:1337	arg1	relationships					1339:1351	similarity relationships	1328:1351	similarity relationships between cancers based on the biochemical composition of serum samples	1328:1421	Subsequently, Super Paramagnetic Clustering method was applied to Raman spectra to study similarity relationships between cancers based on the biochemical composition of serum samples.
30861043	4	86	from	breast	967:972	arg1	leukemia					953:960	discriminating leukemia	938:960	discriminating leukemia from breast and cervical cancer	938:992	According to these spectra, the band associated with amide I (1654 cm-1) and one of two shoulders assigned to amide III (1230-1282 cm-1) allowed discriminating leukemia from breast and cervical cancer, whereas band 714 cm-1 (polysaccharides) achieves to differentiate cervical cancer from leukemia and breast cancer, and bulged region, 1040 - 1100 cm-1 (phenylalanine, phospholipid) discriminated breast cancer from leukemia and cervical cancer.
30861043	3	87	theme	other	710:714	arg1	peaks					716:720	other peaks	710:720	other peaks	710:720	Comparing firstly average Raman spectra of the three cancers, some peaks that allowed differentiating one cancer from other were identified, however, other peaks allowed concluding that there are biochemical similarities among them.
30142561	0	0	theme	vinyl	95:99	arg1	monomer					101:107	vinyl monomer	95:107	vinyl monomer	95:107	Radiation synthesis and characterization of super-absorbing hydrogel from natural polymers and vinyl monomer.
30142561	6	1	theme	hybrid	879:884	arg1	hydrogel					886:893	The prepared hybrid hydrogel	866:893	The prepared hybrid hydrogel	866:893	The prepared hybrid hydrogel showed most optimum swelling capacity at neutral pH whereas equilibrium swelling of SAHs was achieved within 5 h.
30142561	4	2	theme	successful	690:699	arg1	grafting					701:708	successful grafting	690:708	successful grafting of SSS onto the polysaccharide base material	690:753	The FTIR gave characteristic peaks for -SO3Na group at 1042 and 988 cm-1, showing successful grafting of SSS onto the polysaccharide base material.
30142561	6	3	theme	swelling	915:922	arg1	capacity					924:931	most optimum swelling capacity	902:931	most optimum swelling capacity	902:931	The prepared hybrid hydrogel showed most optimum swelling capacity at neutral pH whereas equilibrium swelling of SAHs was achieved within 5 h.
30142561	1	4	theme	polysaccharide-based	164:183	arg1	SAHs					251:254	SAHs	251:254	SAHs	251:254	This article exploits a new approach for synthesis of polysaccharide-based grafted sodium styrene sulfonate (SSS) super absorbent hydrogels (SAHs) in aqueous solution by γ-radiation under ambient conditions.
30142561	1	4	theme	polysaccharide-based	164:183	arg1	hydrogels					240:248	polysaccharide-based grafted sodium styrene sulfonate (SSS) super absorbent hydrogels	164:248	polysaccharide-based grafted sodium styrene sulfonate (SSS) super absorbent hydrogels (SAHs) in aqueous solution	164:275	This article exploits a new approach for synthesis of polysaccharide-based grafted sodium styrene sulfonate (SSS) super absorbent hydrogels (SAHs) in aqueous solution by γ-radiation under ambient conditions.
30142561	7	5	from	percentage	1072:1081	arg1	solution					1086:1093	solution	1086:1093	solution	1086:1093	The swelling of SAHs influenced obviously to metal ion removal percentage in solution.
30142561	5	6	from	behaviors	783:791	arg1	solutions					807:815	various pH solutions	796:815	various pH solutions	796:815	The dependence of swelling behaviors in various pH solutions and salts solutions were investigated in detail.
30142561	5	6	from	behaviors	783:791	arg1	salts					821:825	salts	821:825	salts	821:825	The dependence of swelling behaviors in various pH solutions and salts solutions were investigated in detail.
30142561	4	7	theme	base	741:744	arg1	material					746:753	the polysaccharide base material	722:753	the polysaccharide base material	722:753	The FTIR gave characteristic peaks for -SO3Na group at 1042 and 988 cm-1, showing successful grafting of SSS onto the polysaccharide base material.
30142561	1	8	theme	aqueous	260:266	arg1	solution					268:275	aqueous solution	260:275	aqueous solution	260:275	This article exploits a new approach for synthesis of polysaccharide-based grafted sodium styrene sulfonate (SSS) super absorbent hydrogels (SAHs) in aqueous solution by γ-radiation under ambient conditions.
30142561	4	9	theme	polysaccharide	726:739	arg1	material					746:753	the polysaccharide base material	722:753	the polysaccharide base material	722:753	The FTIR gave characteristic peaks for -SO3Na group at 1042 and 988 cm-1, showing successful grafting of SSS onto the polysaccharide base material.
30142561	4	10	theme	characteristic	622:635	arg1	peaks					637:641	characteristic peaks	622:641	characteristic peaks for -SO3Na group at 1042 and 988 cm-1	622:679	The FTIR gave characteristic peaks for -SO3Na group at 1042 and 988 cm-1, showing successful grafting of SSS onto the polysaccharide base material.
30142561	5	11	dep	solutions	807:815	arg1	solutions					827:835	solutions	827:835	solutions	827:835	The dependence of swelling behaviors in various pH solutions and salts solutions were investigated in detail.
30142561	5	12	from	salts	821:825	arg1	dependence					760:769	The dependence	756:769	The dependence of swelling behaviors in various pH solutions and salts solutions	756:835	The dependence of swelling behaviors in various pH solutions and salts solutions were investigated in detail.
30142561	1	13	from	synthesis	151:159	arg1	solution					268:275	aqueous solution	260:275	aqueous solution	260:275	This article exploits a new approach for synthesis of polysaccharide-based grafted sodium styrene sulfonate (SSS) super absorbent hydrogels (SAHs) in aqueous solution by γ-radiation under ambient conditions.
30142561	4	14	from	1042	663:666	arg1	peaks					637:641	characteristic peaks	622:641	characteristic peaks for -SO3Na group at 1042 and 988 cm-1	622:679	The FTIR gave characteristic peaks for -SO3Na group at 1042 and 988 cm-1, showing successful grafting of SSS onto the polysaccharide base material.
30142561	1	15	theme	grafted	185:191	arg1	SSS					219:221	SSS	219:221	SSS	219:221	This article exploits a new approach for synthesis of polysaccharide-based grafted sodium styrene sulfonate (SSS) super absorbent hydrogels (SAHs) in aqueous solution by γ-radiation under ambient conditions.
30142561	1	15	theme	grafted	185:191	arg1	sulfonate					208:216	grafted sodium styrene sulfonate	185:216	polysaccharide-based grafted sodium styrene sulfonate (SSS) super absorbent hydrogels (SAHs) in aqueous solution	164:275	This article exploits a new approach for synthesis of polysaccharide-based grafted sodium styrene sulfonate (SSS) super absorbent hydrogels (SAHs) in aqueous solution by γ-radiation under ambient conditions.
30142561	2	16	theme	feed	454:457	arg1	composition					459:469	feed composition	454:469	feed composition	454:469	Important optimal conditions for preparation of hydrogels with the best swelling ratio, such as gamma irradiation dose and the ratio of feed composition have been discussed.
30142561	5	17	theme	swelling	774:781	arg1	behaviors					783:791	swelling behaviors	774:791	swelling behaviors in various pH solutions and salts solutions	774:835	The dependence of swelling behaviors in various pH solutions and salts solutions were investigated in detail.
30142561	4	18	from	988 cm-1	672:679	arg1	peaks					637:641	characteristic peaks	622:641	characteristic peaks for -SO3Na group at 1042 and 988 cm-1	622:679	The FTIR gave characteristic peaks for -SO3Na group at 1042 and 988 cm-1, showing successful grafting of SSS onto the polysaccharide base material.
30142561	1	19	theme	sodium	193:198	arg1	SSS					219:221	SSS	219:221	SSS	219:221	This article exploits a new approach for synthesis of polysaccharide-based grafted sodium styrene sulfonate (SSS) super absorbent hydrogels (SAHs) in aqueous solution by γ-radiation under ambient conditions.
30142561	1	19	theme	sodium	193:198	arg1	sulfonate					208:216	grafted sodium styrene sulfonate	185:216	polysaccharide-based grafted sodium styrene sulfonate (SSS) super absorbent hydrogels (SAHs) in aqueous solution	164:275	This article exploits a new approach for synthesis of polysaccharide-based grafted sodium styrene sulfonate (SSS) super absorbent hydrogels (SAHs) in aqueous solution by γ-radiation under ambient conditions.
30142561	5	20	theme	pH	804:805	arg1	solutions					807:815	various pH solutions	796:815	various pH solutions	796:815	The dependence of swelling behaviors in various pH solutions and salts solutions were investigated in detail.
30142561	5	21	from	solutions	807:815	arg1	dependence					760:769	The dependence	756:769	The dependence of swelling behaviors in various pH solutions and salts solutions	756:835	The dependence of swelling behaviors in various pH solutions and salts solutions were investigated in detail.
30142561	2	22	theme	optimal	328:334	arg1	conditions					336:345	Important optimal conditions	318:345	Important optimal conditions for preparation of hydrogels with the best swelling ratio, such as gamma irradiation dose	318:435	Important optimal conditions for preparation of hydrogels with the best swelling ratio, such as gamma irradiation dose and the ratio of feed composition have been discussed.
30142561	1	23	theme	styrene	200:206	arg1	SSS					219:221	SSS	219:221	SSS	219:221	This article exploits a new approach for synthesis of polysaccharide-based grafted sodium styrene sulfonate (SSS) super absorbent hydrogels (SAHs) in aqueous solution by γ-radiation under ambient conditions.
30142561	1	23	theme	styrene	200:206	arg1	sulfonate					208:216	grafted sodium styrene sulfonate	185:216	polysaccharide-based grafted sodium styrene sulfonate (SSS) super absorbent hydrogels (SAHs) in aqueous solution	164:275	This article exploits a new approach for synthesis of polysaccharide-based grafted sodium styrene sulfonate (SSS) super absorbent hydrogels (SAHs) in aqueous solution by γ-radiation under ambient conditions.
30142561	0	24	theme	Radiation	0:8	arg1	synthesis					10:18	Radiation synthesis	0:18	Radiation synthesis	0:18	Radiation synthesis and characterization of super-absorbing hydrogel from natural polymers and vinyl monomer.
30142561	2	25	theme	Important	318:326	arg1	conditions					336:345	Important optimal conditions	318:345	Important optimal conditions for preparation of hydrogels with the best swelling ratio, such as gamma irradiation dose	318:435	Important optimal conditions for preparation of hydrogels with the best swelling ratio, such as gamma irradiation dose and the ratio of feed composition have been discussed.
30142561	1	26	theme	sulfonate	208:216	arg1	SAHs					251:254	SAHs	251:254	SAHs	251:254	This article exploits a new approach for synthesis of polysaccharide-based grafted sodium styrene sulfonate (SSS) super absorbent hydrogels (SAHs) in aqueous solution by γ-radiation under ambient conditions.
30142561	1	26	theme	sulfonate	208:216	arg1	hydrogels					240:248	polysaccharide-based grafted sodium styrene sulfonate (SSS) super absorbent hydrogels	164:248	polysaccharide-based grafted sodium styrene sulfonate (SSS) super absorbent hydrogels (SAHs) in aqueous solution	164:275	This article exploits a new approach for synthesis of polysaccharide-based grafted sodium styrene sulfonate (SSS) super absorbent hydrogels (SAHs) in aqueous solution by γ-radiation under ambient conditions.
30142561	6	27	theme	SAHs	979:982	arg1	swelling					967:974	equilibrium swelling	955:974	equilibrium swelling of SAHs	955:982	The prepared hybrid hydrogel showed most optimum swelling capacity at neutral pH whereas equilibrium swelling of SAHs was achieved within 5 h.
30142561	3	28	used	used	559:562	arg2	SEM/EDS					532:538	the SEM/EDS	528:538	the SEM/EDS	528:538	Characterization techniques such as the SEM/EDS, FTIR and DSC were used in describing the newly prepared hydrogels.
30142561	3	28	used	used	559:562	arg2	techniques					509:518	Characterization techniques	492:518	Characterization techniques such as the SEM/EDS, FTIR and DSC	492:552	Characterization techniques such as the SEM/EDS, FTIR and DSC were used in describing the newly prepared hydrogels.
30142561	1	29	from	solution	268:275	arg1	synthesis					151:159	synthesis	151:159	synthesis of polysaccharide-based grafted sodium styrene sulfonate (SSS) super absorbent hydrogels (SAHs) in aqueous solution	151:275	This article exploits a new approach for synthesis of polysaccharide-based grafted sodium styrene sulfonate (SSS) super absorbent hydrogels (SAHs) in aqueous solution by γ-radiation under ambient conditions.
30142561	6	30	theme	prepared	870:877	arg1	hydrogel					886:893	The prepared hybrid hydrogel	866:893	The prepared hybrid hydrogel	866:893	The prepared hybrid hydrogel showed most optimum swelling capacity at neutral pH whereas equilibrium swelling of SAHs was achieved within 5 h.
30142561	2	31	theme	hydrogels	366:374	arg1	preparation					351:361	preparation	351:361	preparation of hydrogels with the best swelling ratio, such as gamma irradiation dose	351:435	Important optimal conditions for preparation of hydrogels with the best swelling ratio, such as gamma irradiation dose and the ratio of feed composition have been discussed.
30142561	2	32	theme	irradiation	420:430	arg1	dose					432:435	gamma irradiation dose	414:435	gamma irradiation dose	414:435	Important optimal conditions for preparation of hydrogels with the best swelling ratio, such as gamma irradiation dose and the ratio of feed composition have been discussed.
30142561	0	33	theme	super-absorbing	44:58	arg1	hydrogel					60:67	super-absorbing hydrogel	44:67	super-absorbing hydrogel	44:67	Radiation synthesis and characterization of super-absorbing hydrogel from natural polymers and vinyl monomer.
30142561	1	34	theme	ambient	298:304	arg1	conditions					306:315	ambient conditions	298:315	ambient conditions	298:315	This article exploits a new approach for synthesis of polysaccharide-based grafted sodium styrene sulfonate (SSS) super absorbent hydrogels (SAHs) in aqueous solution by γ-radiation under ambient conditions.
30142561	7	35	theme	ion	1060:1062	arg1	percentage					1072:1081	metal ion removal percentage	1054:1081	metal ion removal percentage in solution	1054:1093	The swelling of SAHs influenced obviously to metal ion removal percentage in solution.
30142561	4	36	theme	-SO3Na	647:652	arg1	group					654:658	-SO3Na group	647:658	-SO3Na group	647:658	The FTIR gave characteristic peaks for -SO3Na group at 1042 and 988 cm-1, showing successful grafting of SSS onto the polysaccharide base material.
30142561	3	37	theme	prepared	588:595	arg1	hydrogels					597:605	the newly prepared hydrogels	578:605	the newly prepared hydrogels	578:605	Characterization techniques such as the SEM/EDS, FTIR and DSC were used in describing the newly prepared hydrogels.
30142561	2	38	theme	swelling	390:397	arg1	ratio					399:403	the best swelling ratio	381:403	the best swelling ratio	381:403	Important optimal conditions for preparation of hydrogels with the best swelling ratio, such as gamma irradiation dose and the ratio of feed composition have been discussed.
30142561	2	39	theme	gamma	414:418	arg1	irradiation					420:430	gamma irradiation	414:430	gamma irradiation dose	414:435	Important optimal conditions for preparation of hydrogels with the best swelling ratio, such as gamma irradiation dose and the ratio of feed composition have been discussed.
30142561	5	40	theme	behaviors	783:791	arg1	dependence					760:769	The dependence	756:769	The dependence of swelling behaviors in various pH solutions and salts solutions	756:835	The dependence of swelling behaviors in various pH solutions and salts solutions were investigated in detail.
30142561	6	41	theme	equilibrium	955:965	arg1	swelling					967:974	equilibrium swelling	955:974	equilibrium swelling of SAHs	955:982	The prepared hybrid hydrogel showed most optimum swelling capacity at neutral pH whereas equilibrium swelling of SAHs was achieved within 5 h.
30142561	7	42	theme	removal	1064:1070	arg1	percentage					1072:1081	metal ion removal percentage	1054:1081	metal ion removal percentage in solution	1054:1093	The swelling of SAHs influenced obviously to metal ion removal percentage in solution.
30142561	0	43	from	polymers	82:89	arg1	characterization					24:39	characterization	24:39	characterization	24:39	Radiation synthesis and characterization of super-absorbing hydrogel from natural polymers and vinyl monomer.
30142561	0	43	from	polymers	82:89	arg1	synthesis					10:18	Radiation synthesis	0:18	Radiation synthesis	0:18	Radiation synthesis and characterization of super-absorbing hydrogel from natural polymers and vinyl monomer.
30142561	1	44	theme	super	224:228	arg1	SAHs					251:254	SAHs	251:254	SAHs	251:254	This article exploits a new approach for synthesis of polysaccharide-based grafted sodium styrene sulfonate (SSS) super absorbent hydrogels (SAHs) in aqueous solution by γ-radiation under ambient conditions.
30142561	1	44	theme	super	224:228	arg1	hydrogels					240:248	polysaccharide-based grafted sodium styrene sulfonate (SSS) super absorbent hydrogels	164:248	polysaccharide-based grafted sodium styrene sulfonate (SSS) super absorbent hydrogels (SAHs) in aqueous solution	164:275	This article exploits a new approach for synthesis of polysaccharide-based grafted sodium styrene sulfonate (SSS) super absorbent hydrogels (SAHs) in aqueous solution by γ-radiation under ambient conditions.
30142561	2	45	theme	composition	459:469	arg1	ratio					445:449	the ratio	441:449	the ratio of feed composition	441:469	Important optimal conditions for preparation of hydrogels with the best swelling ratio, such as gamma irradiation dose and the ratio of feed composition have been discussed.
30142561	2	45	theme	composition	459:469	arg1	conditions					336:345	Important optimal conditions	318:345	Important optimal conditions for preparation of hydrogels with the best swelling ratio, such as gamma irradiation dose	318:435	Important optimal conditions for preparation of hydrogels with the best swelling ratio, such as gamma irradiation dose and the ratio of feed composition have been discussed.
30142561	5	46	theme	various	796:802	arg1	solutions					807:815	various pH solutions	796:815	various pH solutions	796:815	The dependence of swelling behaviors in various pH solutions and salts solutions were investigated in detail.
30142561	7	47	theme	SAHs	1025:1028	arg1	swelling					1013:1020	The swelling	1009:1020	The swelling of SAHs	1009:1028	The swelling of SAHs influenced obviously to metal ion removal percentage in solution.
30142561	3	48	theme	Characterization	492:507	arg1	techniques					509:518	Characterization techniques	492:518	Characterization techniques such as the SEM/EDS, FTIR and DSC	492:552	Characterization techniques such as the SEM/EDS, FTIR and DSC were used in describing the newly prepared hydrogels.
30142561	3	48	theme	Characterization	492:507	arg1	SEM/EDS					532:538	the SEM/EDS	528:538	the SEM/EDS	528:538	Characterization techniques such as the SEM/EDS, FTIR and DSC were used in describing the newly prepared hydrogels.
30142561	0	49	theme	hydrogel	60:67	arg1	characterization					24:39	characterization	24:39	characterization	24:39	Radiation synthesis and characterization of super-absorbing hydrogel from natural polymers and vinyl monomer.
30142561	0	49	theme	hydrogel	60:67	arg1	synthesis					10:18	Radiation synthesis	0:18	Radiation synthesis	0:18	Radiation synthesis and characterization of super-absorbing hydrogel from natural polymers and vinyl monomer.
30142561	7	50	theme	metal	1054:1058	arg1	percentage					1072:1081	metal ion removal percentage	1054:1081	metal ion removal percentage in solution	1054:1093	The swelling of SAHs influenced obviously to metal ion removal percentage in solution.
30142561	5	51	from	dependence	760:769	arg1	solutions					807:815	various pH solutions	796:815	various pH solutions	796:815	The dependence of swelling behaviors in various pH solutions and salts solutions were investigated in detail.
30142561	5	51	from	dependence	760:769	arg1	salts					821:825	salts	821:825	salts	821:825	The dependence of swelling behaviors in various pH solutions and salts solutions were investigated in detail.
30142561	6	52	theme	neutral	936:942	arg1	pH					944:945	neutral pH	936:945	neutral pH	936:945	The prepared hybrid hydrogel showed most optimum swelling capacity at neutral pH whereas equilibrium swelling of SAHs was achieved within 5 h.
30142561	2	53	with	hydrogels	366:374	arg1	ratio					399:403	the best swelling ratio	381:403	the best swelling ratio	381:403	Important optimal conditions for preparation of hydrogels with the best swelling ratio, such as gamma irradiation dose and the ratio of feed composition have been discussed.
30142561	1	54	theme	new	134:136	arg1	approach					138:145	a new approach	132:145	a new approach for synthesis of polysaccharide-based grafted sodium styrene sulfonate (SSS) super absorbent hydrogels (SAHs) in aqueous solution	132:275	This article exploits a new approach for synthesis of polysaccharide-based grafted sodium styrene sulfonate (SSS) super absorbent hydrogels (SAHs) in aqueous solution by γ-radiation under ambient conditions.
30142561	1	55	theme	absorbent	230:238	arg1	SAHs					251:254	SAHs	251:254	SAHs	251:254	This article exploits a new approach for synthesis of polysaccharide-based grafted sodium styrene sulfonate (SSS) super absorbent hydrogels (SAHs) in aqueous solution by γ-radiation under ambient conditions.
30142561	1	55	theme	absorbent	230:238	arg1	hydrogels					240:248	polysaccharide-based grafted sodium styrene sulfonate (SSS) super absorbent hydrogels	164:248	polysaccharide-based grafted sodium styrene sulfonate (SSS) super absorbent hydrogels (SAHs) in aqueous solution	164:275	This article exploits a new approach for synthesis of polysaccharide-based grafted sodium styrene sulfonate (SSS) super absorbent hydrogels (SAHs) in aqueous solution by γ-radiation under ambient conditions.
30142561	1	56	from	hydrogels	240:248	arg1	solution					268:275	aqueous solution	260:275	aqueous solution	260:275	This article exploits a new approach for synthesis of polysaccharide-based grafted sodium styrene sulfonate (SSS) super absorbent hydrogels (SAHs) in aqueous solution by γ-radiation under ambient conditions.
30142561	4	57	theme	SSS	713:715	arg1	grafting					701:708	successful grafting	690:708	successful grafting of SSS onto the polysaccharide base material	690:753	The FTIR gave characteristic peaks for -SO3Na group at 1042 and 988 cm-1, showing successful grafting of SSS onto the polysaccharide base material.
30142561	1	58	theme	hydrogels	240:248	arg1	synthesis					151:159	synthesis	151:159	synthesis of polysaccharide-based grafted sodium styrene sulfonate (SSS) super absorbent hydrogels (SAHs) in aqueous solution	151:275	This article exploits a new approach for synthesis of polysaccharide-based grafted sodium styrene sulfonate (SSS) super absorbent hydrogels (SAHs) in aqueous solution by γ-radiation under ambient conditions.
30142561	0	59	from	monomer	101:107	arg1	characterization					24:39	characterization	24:39	characterization	24:39	Radiation synthesis and characterization of super-absorbing hydrogel from natural polymers and vinyl monomer.
30142561	0	59	from	monomer	101:107	arg1	synthesis					10:18	Radiation synthesis	0:18	Radiation synthesis	0:18	Radiation synthesis and characterization of super-absorbing hydrogel from natural polymers and vinyl monomer.
30142561	6	60	theme	optimum	907:913	arg1	capacity					924:931	most optimum swelling capacity	902:931	most optimum swelling capacity	902:931	The prepared hybrid hydrogel showed most optimum swelling capacity at neutral pH whereas equilibrium swelling of SAHs was achieved within 5 h.
30142561	0	61	theme	natural	74:80	arg1	polymers					82:89	natural polymers	74:89	natural polymers	74:89	Radiation synthesis and characterization of super-absorbing hydrogel from natural polymers and vinyl monomer.
29631050	14	0	theme	modified	2466:2473	arg1	pericardium					2483:2493	The genetically modified porcine pericardium	2450:2493	The genetically modified porcine pericardium	2450:2493	The genetically modified porcine pericardium showed reduced immunogenicity but comparable collagen composition and physical characteristics of the pericardium from wild-type pigs.
29631050	2	1	theme	chronic	307:313	arg1	calcification					315:327	chronic calcification	307:327	chronic calcification	307:327	However, recipient immune responses associated with chronic calcification lead to structural valve deterioration (SVD) of BHVs.
29631050	5	2	theme	αGal/Neu5Gc-deficient	852:872	arg1	pigs					874:877	αGal/Neu5Gc-deficient pigs	852:877	αGal/Neu5Gc-deficient pigs	852:877	Moreover, valves from αGal/Neu5Gc-deficient pigs could further reduce human IgM/IgG binding when compared to BHV from αGal-knockout pigs.
29631050	12	3	theme	Bioprosthetic	1930:1942	arg1	BHVs					1958:1961	BHVs	1958:1961	BHVs	1958:1961	Bioprosthetic heart valves (BHVs) made from glutaraldehyde-fixed porcine or bovine tissues are widely used in clinics but exhibit age-dependent structural valve degeneration (SVD) which is associated with the immune response against BHVs.
29631050	12	3	theme	Bioprosthetic	1930:1942	arg1	valves					1950:1955	Bioprosthetic heart valves	1930:1955	Bioprosthetic heart valves (BHVs) made from glutaraldehyde-fixed porcine or bovine tissues	1930:2019	Bioprosthetic heart valves (BHVs) made from glutaraldehyde-fixed porcine or bovine tissues are widely used in clinics but exhibit age-dependent structural valve degeneration (SVD) which is associated with the immune response against BHVs.
29631050	13	4	theme	N-glycolylneuraminic	2256:2275	arg1	acid					2277:2280	N-glycolylneuraminic acid	2256:2280	N-glycolylneuraminic acid (Neu5Gc)	2256:2289	Three major xenoantigens present on commercial BHVs, Galactosea α1,3 galactose (αGal), N-glycolylneuraminic acid (Neu5Gc) and glycan products of β-1,4-N-acetyl-galactosaminyl transferase 2 (β4GalNT2) are eliminated through CRISPR/Cas9 mediated gene targeting in the present study.
29631050	13	4	theme	N-glycolylneuraminic	2256:2275	arg1	Neu5Gc					2283:2288	Neu5Gc	2283:2288	Neu5Gc	2283:2288	Three major xenoantigens present on commercial BHVs, Galactosea α1,3 galactose (αGal), N-glycolylneuraminic acid (Neu5Gc) and glycan products of β-1,4-N-acetyl-galactosaminyl transferase 2 (β4GalNT2) are eliminated through CRISPR/Cas9 mediated gene targeting in the present study.
29631050	2	5	theme	structural	337:346	arg1	SVD					369:371	SVD	369:371	SVD	369:371	However, recipient immune responses associated with chronic calcification lead to structural valve deterioration (SVD) of BHVs.
29631050	2	5	theme	structural	337:346	arg1	deterioration					354:366	structural valve deterioration	337:366	structural valve deterioration (SVD) of BHVs	337:380	However, recipient immune responses associated with chronic calcification lead to structural valve deterioration (SVD) of BHVs.
29631050	13	6	theme	glycan	2295:2300	arg1	products					2302:2309	glycan products	2295:2309	glycan products of β-1,4-N-acetyl-galactosaminyl transferase 2 (β4GalNT2)	2295:2367	Three major xenoantigens present on commercial BHVs, Galactosea α1,3 galactose (αGal), N-glycolylneuraminic acid (Neu5Gc) and glycan products of β-1,4-N-acetyl-galactosaminyl transferase 2 (β4GalNT2) are eliminated through CRISPR/Cas9 mediated gene targeting in the present study.
29631050	12	7	theme	age-dependent	2060:2072	arg1	SVD					2105:2107	SVD	2105:2107	SVD	2105:2107	Bioprosthetic heart valves (BHVs) made from glutaraldehyde-fixed porcine or bovine tissues are widely used in clinics but exhibit age-dependent structural valve degeneration (SVD) which is associated with the immune response against BHVs.
29631050	12	7	theme	age-dependent	2060:2072	arg1	degeneration					2091:2102	age-dependent structural valve degeneration	2060:2102	age-dependent structural valve degeneration (SVD) which is associated with the immune response against BHVs	2060:2166	Bioprosthetic heart valves (BHVs) made from glutaraldehyde-fixed porcine or bovine tissues are widely used in clinics but exhibit age-dependent structural valve degeneration (SVD) which is associated with the immune response against BHVs.
29631050	3	8	theme	1,3	576:578	arg1	galactosyltransferase					581:601	α(1,3) galactosyltransferase	574:601	α(1,3) galactosyltransferase (encoded by the GGTA1 gene)	574:629	Two well-characterized epitopes on porcine BHVs have been implicated in SVD, including galactose-α1,3-galactose (αGal) and N-glycolylneuraminic acid (Neu5Gc) whose synthesis are catalyzed by α(1,3) galactosyltransferase (encoded by the GGTA1 gene) and CMP-Neu5Ac hydroxylase (encoded by the CMAH gene), respectively.
29631050	1	9	theme	heart	219:223	arg1	replacement					231:241	heart valve replacement	219:241	heart valve replacement in clinics	219:252	Bioprosthetic heart valves (BHVs) originating from pigs are extensively used for heart valve replacement in clinics.
29631050	15	10	from	pigs	2668:2671	arg1	alternative					2688:2698	a promising alternative	2676:2698	a promising alternative for currently available BHVs from wild-type pigs	2676:2747	Our data suggested that BHVs from TKO pigs is a promising alternative for currently available BHVs from wild-type pigs.
29631050	15	10	from	pigs	2668:2671	arg1	BHVs					2654:2657	BHVs	2654:2657	BHVs from TKO pigs	2654:2671	Our data suggested that BHVs from TKO pigs is a promising alternative for currently available BHVs from wild-type pigs.
29631050	12	11	theme	valve	2085:2089	arg1	SVD					2105:2107	SVD	2105:2107	SVD	2105:2107	Bioprosthetic heart valves (BHVs) made from glutaraldehyde-fixed porcine or bovine tissues are widely used in clinics but exhibit age-dependent structural valve degeneration (SVD) which is associated with the immune response against BHVs.
29631050	12	11	theme	valve	2085:2089	arg1	degeneration					2091:2102	age-dependent structural valve degeneration	2060:2102	age-dependent structural valve degeneration (SVD) which is associated with the immune response against BHVs	2060:2166	Bioprosthetic heart valves (BHVs) made from glutaraldehyde-fixed porcine or bovine tissues are widely used in clinics but exhibit age-dependent structural valve degeneration (SVD) which is associated with the immune response against BHVs.
29631050	14	12	from	pigs	2624:2627	arg1	characteristics					2574:2588	physical characteristics	2565:2588	physical characteristics	2565:2588	The genetically modified porcine pericardium showed reduced immunogenicity but comparable collagen composition and physical characteristics of the pericardium from wild-type pigs.
29631050	14	12	from	pigs	2624:2627	arg1	composition					2549:2559	comparable collagen composition	2529:2559	comparable collagen composition	2529:2559	The genetically modified porcine pericardium showed reduced immunogenicity but comparable collagen composition and physical characteristics of the pericardium from wild-type pigs.
29631050	7	13	theme	CMAH	1139:1142	arg1	pigs					1185:1188	GGTA1, CMAH, and β4GalNT2 triple gene-knockout (TKO) pigs	1132:1188	GGTA1, CMAH, and β4GalNT2 triple gene-knockout (TKO) pigs	1132:1188	To explore whether tissue from GGTA1, CMAH, and β4GalNT2 triple gene-knockout (TKO) pigs would further minimize human antibody binding to porcine pericardium, TKO pigs were successfully produced by CRISPR/Cas9 mediated gene targeting.
29631050	9	14	theme	significant	1662:1672	arg1	impact					1674:1679	no significant impact	1659:1679	no significant impact	1659:1679	Moreover, the analysis of collagen composition and physical characteristics of porcine pericardium from the TKO pigs indicated that elimination of the three xenoantigens had no significant impact on the physical proprieties of porcine pericardium.
29631050	13	15	theme	present	2194:2200	arg1	xenoantigens					2181:2192	Three major xenoantigens	2169:2192	Three major xenoantigens present on commercial BHVs, Galactosea α1,3 galactose (αGal), N-glycolylneuraminic acid (Neu5Gc) and glycan products of β-1,4-N-acetyl-galactosaminyl transferase 2 (β4GalNT2)	2169:2367	Three major xenoantigens present on commercial BHVs, Galactosea α1,3 galactose (αGal), N-glycolylneuraminic acid (Neu5Gc) and glycan products of β-1,4-N-acetyl-galactosaminyl transferase 2 (β4GalNT2) are eliminated through CRISPR/Cas9 mediated gene targeting in the present study.
29631050	12	16	used	used	2032:2035	arg2	valves					1950:1955	Bioprosthetic heart valves	1930:1955	Bioprosthetic heart valves (BHVs) made from glutaraldehyde-fixed porcine or bovine tissues	1930:2019	Bioprosthetic heart valves (BHVs) made from glutaraldehyde-fixed porcine or bovine tissues are widely used in clinics but exhibit age-dependent structural valve degeneration (SVD) which is associated with the immune response against BHVs.
29631050	12	16	used	used	2032:2035	arg2	BHVs					1958:1961	BHVs	1958:1961	BHVs	1958:1961	Bioprosthetic heart valves (BHVs) made from glutaraldehyde-fixed porcine or bovine tissues are widely used in clinics but exhibit age-dependent structural valve degeneration (SVD) which is associated with the immune response against BHVs.
29631050	13	17	theme	present	2435:2441	arg1	study					2443:2447	the present study	2431:2447	the present study	2431:2447	Three major xenoantigens present on commercial BHVs, Galactosea α1,3 galactose (αGal), N-glycolylneuraminic acid (Neu5Gc) and glycan products of β-1,4-N-acetyl-galactosaminyl transferase 2 (β4GalNT2) are eliminated through CRISPR/Cas9 mediated gene targeting in the present study.
29631050	11	18	theme	diseased	1908:1915	arg1	valve					1923:1927	diseased heart valve	1908:1927	diseased heart valve	1908:1927	STATEMENT OF SIGNIFICANCE Surgical heart valve replacement is an established lifesaving treatment for diseased heart valve.
29631050	1	19	theme	heart	152:156	arg1	valves					158:163	Bioprosthetic heart valves	138:163	Bioprosthetic heart valves (BHVs) originating from pigs	138:192	Bioprosthetic heart valves (BHVs) originating from pigs are extensively used for heart valve replacement in clinics.
29631050	1	19	theme	heart	152:156	arg1	BHVs					166:169	BHVs	166:169	BHVs	166:169	Bioprosthetic heart valves (BHVs) originating from pigs are extensively used for heart valve replacement in clinics.
29631050	7	20	from	pigs	1185:1188	arg1	tissue					1120:1125	tissue	1120:1125	tissue from GGTA1, CMAH, and β4GalNT2 triple gene-knockout (TKO) pigs	1120:1188	To explore whether tissue from GGTA1, CMAH, and β4GalNT2 triple gene-knockout (TKO) pigs would further minimize human antibody binding to porcine pericardium, TKO pigs were successfully produced by CRISPR/Cas9 mediated gene targeting.
29631050	1	21	used	used	210:213	arg2	BHVs					166:169	BHVs	166:169	BHVs	166:169	Bioprosthetic heart valves (BHVs) originating from pigs are extensively used for heart valve replacement in clinics.
29631050	1	21	used	used	210:213	arg2	valves					158:163	Bioprosthetic heart valves	138:163	Bioprosthetic heart valves (BHVs) originating from pigs	138:192	Bioprosthetic heart valves (BHVs) originating from pigs are extensively used for heart valve replacement in clinics.
29631050	2	22	theme	immune	274:279	arg1	responses					281:289	recipient immune responses	264:289	recipient immune responses associated with chronic calcification	264:327	However, recipient immune responses associated with chronic calcification lead to structural valve deterioration (SVD) of BHVs.
29631050	5	23	from	pigs	874:877	arg1	valves					840:845	valves	840:845	valves from αGal/Neu5Gc-deficient pigs	840:877	Moreover, valves from αGal/Neu5Gc-deficient pigs could further reduce human IgM/IgG binding when compared to BHV from αGal-knockout pigs.
29631050	9	24	theme	physical	1688:1695	arg1	proprieties					1697:1707	the physical proprieties	1684:1707	the physical proprieties of porcine pericardium	1684:1730	Moreover, the analysis of collagen composition and physical characteristics of porcine pericardium from the TKO pigs indicated that elimination of the three xenoantigens had no significant impact on the physical proprieties of porcine pericardium.
29631050	7	25	theme	gene	1320:1323	arg1	targeting					1325:1333	CRISPR/Cas9 mediated gene targeting	1299:1333	CRISPR/Cas9 mediated gene targeting	1299:1333	To explore whether tissue from GGTA1, CMAH, and β4GalNT2 triple gene-knockout (TKO) pigs would further minimize human antibody binding to porcine pericardium, TKO pigs were successfully produced by CRISPR/Cas9 mediated gene targeting.
29631050	0	26	theme	major	94:98	arg1	GGTA1/β4GalNT2/CMAH					117:135	GGTA1/β4GalNT2/CMAH	117:135	GGTA1/β4GalNT2/CMAH	117:135	Reducing immunoreactivity of porcine bioprosthetic heart valves by genetically-deleting three major glycan antigens, GGTA1/β4GalNT2/CMAH.
29631050	0	26	theme	major	94:98	arg1	antigens					107:114	three major glycan antigens	88:114	three major glycan antigens	88:114	Reducing immunoreactivity of porcine bioprosthetic heart valves by genetically-deleting three major glycan antigens, GGTA1/β4GalNT2/CMAH.
29631050	5	27	theme	IgM/IgG	906:912	arg1	binding					914:920	human IgM/IgG binding	900:920	human IgM/IgG binding	900:920	Moreover, valves from αGal/Neu5Gc-deficient pigs could further reduce human IgM/IgG binding when compared to BHV from αGal-knockout pigs.
29631050	9	28	theme	pericardium	1720:1730	arg1	proprieties					1697:1707	the physical proprieties	1684:1707	the physical proprieties of porcine pericardium	1684:1730	Moreover, the analysis of collagen composition and physical characteristics of porcine pericardium from the TKO pigs indicated that elimination of the three xenoantigens had no significant impact on the physical proprieties of porcine pericardium.
29631050	8	29	theme	human	1435:1439	arg1	binding					1449:1455	human IgG/IgM binding	1435:1455	human IgG/IgM binding to pericardium	1435:1470	Our results showed that the expression of αGal, Neu5G and Sd(a) on TKO pigs was negative, and that human IgG/IgM binding to pericardium was minimal.
29631050	13	30	theme	CRISPR/Cas9	2392:2402	arg1	targeting					2418:2426	CRISPR/Cas9 mediated gene targeting	2392:2426	CRISPR/Cas9 mediated gene targeting in the present study	2392:2447	Three major xenoantigens present on commercial BHVs, Galactosea α1,3 galactose (αGal), N-glycolylneuraminic acid (Neu5Gc) and glycan products of β-1,4-N-acetyl-galactosaminyl transferase 2 (β4GalNT2) are eliminated through CRISPR/Cas9 mediated gene targeting in the present study.
29631050	7	31	theme	TKO	1180:1182	arg1	pigs					1185:1188	GGTA1, CMAH, and β4GalNT2 triple gene-knockout (TKO) pigs	1132:1188	GGTA1, CMAH, and β4GalNT2 triple gene-knockout (TKO) pigs	1132:1188	To explore whether tissue from GGTA1, CMAH, and β4GalNT2 triple gene-knockout (TKO) pigs would further minimize human antibody binding to porcine pericardium, TKO pigs were successfully produced by CRISPR/Cas9 mediated gene targeting.
29631050	3	32	theme	α	574:574	arg1	galactosyltransferase					581:601	α(1,3) galactosyltransferase	574:601	α(1,3) galactosyltransferase (encoded by the GGTA1 gene)	574:629	Two well-characterized epitopes on porcine BHVs have been implicated in SVD, including galactose-α1,3-galactose (αGal) and N-glycolylneuraminic acid (Neu5Gc) whose synthesis are catalyzed by α(1,3) galactosyltransferase (encoded by the GGTA1 gene) and CMP-Neu5Ac hydroxylase (encoded by the CMAH gene), respectively.
29631050	13	33	theme	gene	2413:2416	arg1	targeting					2418:2426	CRISPR/Cas9 mediated gene targeting	2392:2426	CRISPR/Cas9 mediated gene targeting in the present study	2392:2447	Three major xenoantigens present on commercial BHVs, Galactosea α1,3 galactose (αGal), N-glycolylneuraminic acid (Neu5Gc) and glycan products of β-1,4-N-acetyl-galactosaminyl transferase 2 (β4GalNT2) are eliminated through CRISPR/Cas9 mediated gene targeting in the present study.
29631050	6	34	theme	swine	986:990	arg1	Sd					1005:1006	Sd	1005:1006	Sd(a)	1005:1009	Recently, another swine xenoantigen, Sd(a), produced by β-1,4-N-acetyl-galactosaminyl transferase 2 (β4GalNT2), has been identified.
29631050	6	34	theme	swine	986:990	arg1	xenoantigen					992:1002	another swine xenoantigen	978:1002	another swine xenoantigen	978:1002	Recently, another swine xenoantigen, Sd(a), produced by β-1,4-N-acetyl-galactosaminyl transferase 2 (β4GalNT2), has been identified.
29631050	11	35	theme	replacement	1853:1863	arg1	STATEMENT					1806:1814	STATEMENT	1806:1814	STATEMENT OF SIGNIFICANCE Surgical heart valve replacement	1806:1863	STATEMENT OF SIGNIFICANCE Surgical heart valve replacement is an established lifesaving treatment for diseased heart valve.
29631050	11	35	theme	replacement	1853:1863	arg1	treatment					1894:1902	an established lifesaving treatment	1868:1902	an established lifesaving treatment for diseased heart valve	1868:1927	STATEMENT OF SIGNIFICANCE Surgical heart valve replacement is an established lifesaving treatment for diseased heart valve.
29631050	7	36	theme	gene-knockout	1165:1177	arg1	pigs					1185:1188	GGTA1, CMAH, and β4GalNT2 triple gene-knockout (TKO) pigs	1132:1188	GGTA1, CMAH, and β4GalNT2 triple gene-knockout (TKO) pigs	1132:1188	To explore whether tissue from GGTA1, CMAH, and β4GalNT2 triple gene-knockout (TKO) pigs would further minimize human antibody binding to porcine pericardium, TKO pigs were successfully produced by CRISPR/Cas9 mediated gene targeting.
29631050	0	37	theme	heart	51:55	arg1	valves					57:62	porcine bioprosthetic heart valves	29:62	porcine bioprosthetic heart valves	29:62	Reducing immunoreactivity of porcine bioprosthetic heart valves by genetically-deleting three major glycan antigens, GGTA1/β4GalNT2/CMAH.
29631050	7	38	theme	mediated	1311:1318	arg1	targeting					1325:1333	CRISPR/Cas9 mediated gene targeting	1299:1333	CRISPR/Cas9 mediated gene targeting	1299:1333	To explore whether tissue from GGTA1, CMAH, and β4GalNT2 triple gene-knockout (TKO) pigs would further minimize human antibody binding to porcine pericardium, TKO pigs were successfully produced by CRISPR/Cas9 mediated gene targeting.
29631050	11	39	theme	heart	1841:1845	arg1	replacement					1853:1863	SIGNIFICANCE Surgical heart valve replacement	1819:1863	SIGNIFICANCE Surgical heart valve replacement	1819:1863	STATEMENT OF SIGNIFICANCE Surgical heart valve replacement is an established lifesaving treatment for diseased heart valve.
29631050	7	40	theme	β4GalNT2	1149:1156	arg1	pigs					1185:1188	GGTA1, CMAH, and β4GalNT2 triple gene-knockout (TKO) pigs	1132:1188	GGTA1, CMAH, and β4GalNT2 triple gene-knockout (TKO) pigs	1132:1188	To explore whether tissue from GGTA1, CMAH, and β4GalNT2 triple gene-knockout (TKO) pigs would further minimize human antibody binding to porcine pericardium, TKO pigs were successfully produced by CRISPR/Cas9 mediated gene targeting.
29631050	3	41	theme	well-characterized	387:404	arg1	epitopes					406:413	Two well-characterized epitopes	383:413	Two well-characterized epitopes on porcine BHVs	383:429	Two well-characterized epitopes on porcine BHVs have been implicated in SVD, including galactose-α1,3-galactose (αGal) and N-glycolylneuraminic acid (Neu5Gc) whose synthesis are catalyzed by α(1,3) galactosyltransferase (encoded by the GGTA1 gene) and CMP-Neu5Ac hydroxylase (encoded by the CMAH gene), respectively.
29631050	3	42	theme	GGTA1	619:623	arg1	gene					625:628	the GGTA1 gene	615:628	the GGTA1 gene	615:628	Two well-characterized epitopes on porcine BHVs have been implicated in SVD, including galactose-α1,3-galactose (αGal) and N-glycolylneuraminic acid (Neu5Gc) whose synthesis are catalyzed by α(1,3) galactosyltransferase (encoded by the GGTA1 gene) and CMP-Neu5Ac hydroxylase (encoded by the CMAH gene), respectively.
29631050	4	43	theme	αGal-knockout	735:747	arg1	pigs					749:752	αGal-knockout pigs	735:752	αGal-knockout pigs	735:752	It has been reported that BHV from αGal-knockout pigs are associated with a significantly reduced immune response by human serum.
29631050	11	44	theme	lifesaving	1883:1892	arg1	STATEMENT					1806:1814	STATEMENT	1806:1814	STATEMENT OF SIGNIFICANCE Surgical heart valve replacement	1806:1863	STATEMENT OF SIGNIFICANCE Surgical heart valve replacement is an established lifesaving treatment for diseased heart valve.
29631050	11	44	theme	lifesaving	1883:1892	arg1	treatment					1894:1902	an established lifesaving treatment	1868:1902	an established lifesaving treatment for diseased heart valve	1868:1927	STATEMENT OF SIGNIFICANCE Surgical heart valve replacement is an established lifesaving treatment for diseased heart valve.
29631050	9	45	theme	collagen	1511:1518	arg1	composition					1520:1530	collagen composition	1511:1530	collagen composition	1511:1530	Moreover, the analysis of collagen composition and physical characteristics of porcine pericardium from the TKO pigs indicated that elimination of the three xenoantigens had no significant impact on the physical proprieties of porcine pericardium.
29631050	9	46	from	composition	1520:1530	arg1	pigs					1597:1600	the TKO pigs	1589:1600	the TKO pigs	1589:1600	Moreover, the analysis of collagen composition and physical characteristics of porcine pericardium from the TKO pigs indicated that elimination of the three xenoantigens had no significant impact on the physical proprieties of porcine pericardium.
29631050	6	47	theme	β-1,4-N-acetyl-galactosaminyl	1024:1052	arg1	transferase					1054:1064	β-1,4-N-acetyl-galactosaminyl transferase 2	1024:1066	β-1,4-N-acetyl-galactosaminyl transferase 2 (β4GalNT2)	1024:1077	Recently, another swine xenoantigen, Sd(a), produced by β-1,4-N-acetyl-galactosaminyl transferase 2 (β4GalNT2), has been identified.
29631050	6	47	theme	β-1,4-N-acetyl-galactosaminyl	1024:1052	arg1	β4GalNT2					1069:1076	β4GalNT2	1069:1076	β4GalNT2	1069:1076	Recently, another swine xenoantigen, Sd(a), produced by β-1,4-N-acetyl-galactosaminyl transferase 2 (β4GalNT2), has been identified.
29631050	10	48	theme	BHVs	1800:1803	arg1	source					1790:1795	an ideal source	1781:1795	an ideal source of BHVs	1781:1803	Our results demonstrated that TKO pigs would be an ideal source of BHVs.
29631050	10	48	theme	BHVs	1800:1803	arg1	pigs					1767:1770	TKO pigs	1763:1770	TKO pigs	1763:1770	Our results demonstrated that TKO pigs would be an ideal source of BHVs.
29631050	12	49	theme	immune	2139:2144	arg1	response					2146:2153	the immune response	2135:2153	the immune response against BHVs	2135:2166	Bioprosthetic heart valves (BHVs) made from glutaraldehyde-fixed porcine or bovine tissues are widely used in clinics but exhibit age-dependent structural valve degeneration (SVD) which is associated with the immune response against BHVs.
29631050	0	50	theme	bioprosthetic	37:49	arg1	valves					57:62	porcine bioprosthetic heart valves	29:62	porcine bioprosthetic heart valves	29:62	Reducing immunoreactivity of porcine bioprosthetic heart valves by genetically-deleting three major glycan antigens, GGTA1/β4GalNT2/CMAH.
29631050	15	51	theme	wild-type	2734:2742	arg1	pigs					2744:2747	wild-type pigs	2734:2747	wild-type pigs	2734:2747	Our data suggested that BHVs from TKO pigs is a promising alternative for currently available BHVs from wild-type pigs.
29631050	14	52	theme	comparable	2529:2538	arg1	composition					2549:2559	comparable collagen composition	2529:2559	comparable collagen composition	2529:2559	The genetically modified porcine pericardium showed reduced immunogenicity but comparable collagen composition and physical characteristics of the pericardium from wild-type pigs.
29631050	0	53	theme	valves	57:62	arg1	immunoreactivity					9:24	immunoreactivity	9:24	immunoreactivity of porcine bioprosthetic heart valves	9:62	Reducing immunoreactivity of porcine bioprosthetic heart valves by genetically-deleting three major glycan antigens, GGTA1/β4GalNT2/CMAH.
29631050	9	54	theme	characteristics	1545:1559	arg1	analysis					1499:1506	the analysis	1495:1506	the analysis of collagen composition and physical characteristics of porcine pericardium from the TKO pigs	1495:1600	Moreover, the analysis of collagen composition and physical characteristics of porcine pericardium from the TKO pigs indicated that elimination of the three xenoantigens had no significant impact on the physical proprieties of porcine pericardium.
29631050	5	55	from	pigs	962:965	arg1	BHV					939:941	BHV	939:941	BHV from αGal-knockout pigs	939:965	Moreover, valves from αGal/Neu5Gc-deficient pigs could further reduce human IgM/IgG binding when compared to BHV from αGal-knockout pigs.
29631050	9	56	theme	porcine	1564:1570	arg1	pericardium					1572:1582	porcine pericardium	1564:1582	porcine pericardium from the TKO pigs	1564:1600	Moreover, the analysis of collagen composition and physical characteristics of porcine pericardium from the TKO pigs indicated that elimination of the three xenoantigens had no significant impact on the physical proprieties of porcine pericardium.
29631050	7	57	theme	porcine	1239:1245	arg1	pericardium					1247:1257	porcine pericardium	1239:1257	porcine pericardium	1239:1257	To explore whether tissue from GGTA1, CMAH, and β4GalNT2 triple gene-knockout (TKO) pigs would further minimize human antibody binding to porcine pericardium, TKO pigs were successfully produced by CRISPR/Cas9 mediated gene targeting.
29631050	4	58	theme	immune	798:803	arg1	response					805:812	a significantly reduced immune response	774:812	a significantly reduced immune response by human serum	774:827	It has been reported that BHV from αGal-knockout pigs are associated with a significantly reduced immune response by human serum.
29631050	11	59	theme	SIGNIFICANCE	1819:1830	arg1	replacement					1853:1863	SIGNIFICANCE Surgical heart valve replacement	1819:1863	SIGNIFICANCE Surgical heart valve replacement	1819:1863	STATEMENT OF SIGNIFICANCE Surgical heart valve replacement is an established lifesaving treatment for diseased heart valve.
29631050	14	60	theme	porcine	2475:2481	arg1	pericardium					2483:2493	The genetically modified porcine pericardium	2450:2493	The genetically modified porcine pericardium	2450:2493	The genetically modified porcine pericardium showed reduced immunogenicity but comparable collagen composition and physical characteristics of the pericardium from wild-type pigs.
29631050	13	61	theme	transferase	2344:2354	arg1	BHVs					2216:2219	commercial BHVs	2205:2219	commercial BHVs	2205:2219	Three major xenoantigens present on commercial BHVs, Galactosea α1,3 galactose (αGal), N-glycolylneuraminic acid (Neu5Gc) and glycan products of β-1,4-N-acetyl-galactosaminyl transferase 2 (β4GalNT2) are eliminated through CRISPR/Cas9 mediated gene targeting in the present study.
29631050	13	61	theme	transferase	2344:2354	arg1	acid					2277:2280	N-glycolylneuraminic acid	2256:2280	N-glycolylneuraminic acid (Neu5Gc)	2256:2289	Three major xenoantigens present on commercial BHVs, Galactosea α1,3 galactose (αGal), N-glycolylneuraminic acid (Neu5Gc) and glycan products of β-1,4-N-acetyl-galactosaminyl transferase 2 (β4GalNT2) are eliminated through CRISPR/Cas9 mediated gene targeting in the present study.
29631050	13	61	theme	transferase	2344:2354	arg1	products					2302:2309	glycan products	2295:2309	glycan products of β-1,4-N-acetyl-galactosaminyl transferase 2 (β4GalNT2)	2295:2367	Three major xenoantigens present on commercial BHVs, Galactosea α1,3 galactose (αGal), N-glycolylneuraminic acid (Neu5Gc) and glycan products of β-1,4-N-acetyl-galactosaminyl transferase 2 (β4GalNT2) are eliminated through CRISPR/Cas9 mediated gene targeting in the present study.
29631050	13	61	theme	transferase	2344:2354	arg1	galactose					2238:2246	Galactosea α1,3 galactose	2222:2246	Galactosea α1,3 galactose (αGal)	2222:2253	Three major xenoantigens present on commercial BHVs, Galactosea α1,3 galactose (αGal), N-glycolylneuraminic acid (Neu5Gc) and glycan products of β-1,4-N-acetyl-galactosaminyl transferase 2 (β4GalNT2) are eliminated through CRISPR/Cas9 mediated gene targeting in the present study.
29631050	13	61	theme	transferase	2344:2354	arg1	αGal					2249:2252	αGal	2249:2252	αGal	2249:2252	Three major xenoantigens present on commercial BHVs, Galactosea α1,3 galactose (αGal), N-glycolylneuraminic acid (Neu5Gc) and glycan products of β-1,4-N-acetyl-galactosaminyl transferase 2 (β4GalNT2) are eliminated through CRISPR/Cas9 mediated gene targeting in the present study.
29631050	13	61	theme	transferase	2344:2354	arg1	Neu5Gc					2283:2288	Neu5Gc	2283:2288	Neu5Gc	2283:2288	Three major xenoantigens present on commercial BHVs, Galactosea α1,3 galactose (αGal), N-glycolylneuraminic acid (Neu5Gc) and glycan products of β-1,4-N-acetyl-galactosaminyl transferase 2 (β4GalNT2) are eliminated through CRISPR/Cas9 mediated gene targeting in the present study.
29631050	15	62	theme	promising	2678:2686	arg1	alternative					2688:2698	a promising alternative	2676:2698	a promising alternative for currently available BHVs from wild-type pigs	2676:2747	Our data suggested that BHVs from TKO pigs is a promising alternative for currently available BHVs from wild-type pigs.
29631050	15	62	theme	promising	2678:2686	arg1	BHVs					2654:2657	BHVs	2654:2657	BHVs from TKO pigs	2654:2671	Our data suggested that BHVs from TKO pigs is a promising alternative for currently available BHVs from wild-type pigs.
29631050	2	63	theme	BHVs	377:380	arg1	SVD					369:371	SVD	369:371	SVD	369:371	However, recipient immune responses associated with chronic calcification lead to structural valve deterioration (SVD) of BHVs.
29631050	2	63	theme	BHVs	377:380	arg1	deterioration					354:366	structural valve deterioration	337:366	structural valve deterioration (SVD) of BHVs	337:380	However, recipient immune responses associated with chronic calcification lead to structural valve deterioration (SVD) of BHVs.
29631050	9	64	theme	TKO	1593:1595	arg1	pigs					1597:1600	the TKO pigs	1589:1600	the TKO pigs	1589:1600	Moreover, the analysis of collagen composition and physical characteristics of porcine pericardium from the TKO pigs indicated that elimination of the three xenoantigens had no significant impact on the physical proprieties of porcine pericardium.
29631050	7	65	theme	human	1213:1217	arg1	binding					1228:1234	human antibody binding	1213:1234	human antibody binding to porcine pericardium	1213:1257	To explore whether tissue from GGTA1, CMAH, and β4GalNT2 triple gene-knockout (TKO) pigs would further minimize human antibody binding to porcine pericardium, TKO pigs were successfully produced by CRISPR/Cas9 mediated gene targeting.
29631050	13	66	theme	Galactosea	2222:2231	arg1	αGal					2249:2252	αGal	2249:2252	αGal	2249:2252	Three major xenoantigens present on commercial BHVs, Galactosea α1,3 galactose (αGal), N-glycolylneuraminic acid (Neu5Gc) and glycan products of β-1,4-N-acetyl-galactosaminyl transferase 2 (β4GalNT2) are eliminated through CRISPR/Cas9 mediated gene targeting in the present study.
29631050	13	66	theme	Galactosea	2222:2231	arg1	galactose					2238:2246	Galactosea α1,3 galactose	2222:2246	Galactosea α1,3 galactose (αGal)	2222:2253	Three major xenoantigens present on commercial BHVs, Galactosea α1,3 galactose (αGal), N-glycolylneuraminic acid (Neu5Gc) and glycan products of β-1,4-N-acetyl-galactosaminyl transferase 2 (β4GalNT2) are eliminated through CRISPR/Cas9 mediated gene targeting in the present study.
29631050	2	67	theme	valve	348:352	arg1	SVD					369:371	SVD	369:371	SVD	369:371	However, recipient immune responses associated with chronic calcification lead to structural valve deterioration (SVD) of BHVs.
29631050	2	67	theme	valve	348:352	arg1	deterioration					354:366	structural valve deterioration	337:366	structural valve deterioration (SVD) of BHVs	337:380	However, recipient immune responses associated with chronic calcification lead to structural valve deterioration (SVD) of BHVs.
29631050	8	68	from	expression	1364:1373	arg1	pigs					1407:1410	TKO pigs	1403:1410	TKO pigs	1403:1410	Our results showed that the expression of αGal, Neu5G and Sd(a) on TKO pigs was negative, and that human IgG/IgM binding to pericardium was minimal.
29631050	4	69	theme	human	817:821	arg1	serum					823:827	human serum	817:827	human serum	817:827	It has been reported that BHV from αGal-knockout pigs are associated with a significantly reduced immune response by human serum.
29631050	12	70	theme	heart	1944:1948	arg1	BHVs					1958:1961	BHVs	1958:1961	BHVs	1958:1961	Bioprosthetic heart valves (BHVs) made from glutaraldehyde-fixed porcine or bovine tissues are widely used in clinics but exhibit age-dependent structural valve degeneration (SVD) which is associated with the immune response against BHVs.
29631050	12	70	theme	heart	1944:1948	arg1	valves					1950:1955	Bioprosthetic heart valves	1930:1955	Bioprosthetic heart valves (BHVs) made from glutaraldehyde-fixed porcine or bovine tissues	1930:2019	Bioprosthetic heart valves (BHVs) made from glutaraldehyde-fixed porcine or bovine tissues are widely used in clinics but exhibit age-dependent structural valve degeneration (SVD) which is associated with the immune response against BHVs.
29631050	9	71	theme	xenoantigens	1642:1653	arg1	elimination					1617:1627	elimination	1617:1627	elimination of the three xenoantigens	1617:1653	Moreover, the analysis of collagen composition and physical characteristics of porcine pericardium from the TKO pigs indicated that elimination of the three xenoantigens had no significant impact on the physical proprieties of porcine pericardium.
29631050	13	72	theme	α1,3	2233:2236	arg1	αGal					2249:2252	αGal	2249:2252	αGal	2249:2252	Three major xenoantigens present on commercial BHVs, Galactosea α1,3 galactose (αGal), N-glycolylneuraminic acid (Neu5Gc) and glycan products of β-1,4-N-acetyl-galactosaminyl transferase 2 (β4GalNT2) are eliminated through CRISPR/Cas9 mediated gene targeting in the present study.
29631050	13	72	theme	α1,3	2233:2236	arg1	galactose					2238:2246	Galactosea α1,3 galactose	2222:2246	Galactosea α1,3 galactose (αGal)	2222:2253	Three major xenoantigens present on commercial BHVs, Galactosea α1,3 galactose (αGal), N-glycolylneuraminic acid (Neu5Gc) and glycan products of β-1,4-N-acetyl-galactosaminyl transferase 2 (β4GalNT2) are eliminated through CRISPR/Cas9 mediated gene targeting in the present study.
29631050	11	73	theme	heart	1917:1921	arg1	valve					1923:1927	diseased heart valve	1908:1927	diseased heart valve	1908:1927	STATEMENT OF SIGNIFICANCE Surgical heart valve replacement is an established lifesaving treatment for diseased heart valve.
29631050	1	74	theme	valve	225:229	arg1	replacement					231:241	heart valve replacement	219:241	heart valve replacement in clinics	219:252	Bioprosthetic heart valves (BHVs) originating from pigs are extensively used for heart valve replacement in clinics.
29631050	9	75	from	pigs	1597:1600	arg1	composition					1520:1530	collagen composition	1511:1530	collagen composition	1511:1530	Moreover, the analysis of collagen composition and physical characteristics of porcine pericardium from the TKO pigs indicated that elimination of the three xenoantigens had no significant impact on the physical proprieties of porcine pericardium.
29631050	9	75	from	pigs	1597:1600	arg1	characteristics					1545:1559	physical characteristics	1536:1559	physical characteristics	1536:1559	Moreover, the analysis of collagen composition and physical characteristics of porcine pericardium from the TKO pigs indicated that elimination of the three xenoantigens had no significant impact on the physical proprieties of porcine pericardium.
29631050	9	75	from	pigs	1597:1600	arg1	pericardium					1572:1582	porcine pericardium	1564:1582	porcine pericardium from the TKO pigs	1564:1600	Moreover, the analysis of collagen composition and physical characteristics of porcine pericardium from the TKO pigs indicated that elimination of the three xenoantigens had no significant impact on the physical proprieties of porcine pericardium.
29631050	8	76	theme	TKO	1403:1405	arg1	pigs					1407:1410	TKO pigs	1403:1410	TKO pigs	1403:1410	Our results showed that the expression of αGal, Neu5G and Sd(a) on TKO pigs was negative, and that human IgG/IgM binding to pericardium was minimal.
29631050	12	77	theme	structural	2074:2083	arg1	SVD					2105:2107	SVD	2105:2107	SVD	2105:2107	Bioprosthetic heart valves (BHVs) made from glutaraldehyde-fixed porcine or bovine tissues are widely used in clinics but exhibit age-dependent structural valve degeneration (SVD) which is associated with the immune response against BHVs.
29631050	12	77	theme	structural	2074:2083	arg1	degeneration					2091:2102	age-dependent structural valve degeneration	2060:2102	age-dependent structural valve degeneration (SVD) which is associated with the immune response against BHVs	2060:2166	Bioprosthetic heart valves (BHVs) made from glutaraldehyde-fixed porcine or bovine tissues are widely used in clinics but exhibit age-dependent structural valve degeneration (SVD) which is associated with the immune response against BHVs.
29631050	1	78	from	replacement	231:241	arg1	clinics					246:252	clinics	246:252	clinics	246:252	Bioprosthetic heart valves (BHVs) originating from pigs are extensively used for heart valve replacement in clinics.
29631050	1	79	theme	Bioprosthetic	138:150	arg1	valves					158:163	Bioprosthetic heart valves	138:163	Bioprosthetic heart valves (BHVs) originating from pigs	138:192	Bioprosthetic heart valves (BHVs) originating from pigs are extensively used for heart valve replacement in clinics.
29631050	1	79	theme	Bioprosthetic	138:150	arg1	BHVs					166:169	BHVs	166:169	BHVs	166:169	Bioprosthetic heart valves (BHVs) originating from pigs are extensively used for heart valve replacement in clinics.
29631050	10	80	theme	ideal	1784:1788	arg1	source					1790:1795	an ideal source	1781:1795	an ideal source of BHVs	1781:1803	Our results demonstrated that TKO pigs would be an ideal source of BHVs.
29631050	10	80	theme	ideal	1784:1788	arg1	pigs					1767:1770	TKO pigs	1763:1770	TKO pigs	1763:1770	Our results demonstrated that TKO pigs would be an ideal source of BHVs.
29631050	14	81	theme	wild-type	2614:2622	arg1	pigs					2624:2627	wild-type pigs	2614:2627	wild-type pigs	2614:2627	The genetically modified porcine pericardium showed reduced immunogenicity but comparable collagen composition and physical characteristics of the pericardium from wild-type pigs.
29631050	2	82	theme	recipient	264:272	arg1	responses					281:289	recipient immune responses	264:289	recipient immune responses associated with chronic calcification	264:327	However, recipient immune responses associated with chronic calcification lead to structural valve deterioration (SVD) of BHVs.
29631050	14	83	theme	pericardium	2597:2607	arg1	characteristics					2574:2588	physical characteristics	2565:2588	physical characteristics	2565:2588	The genetically modified porcine pericardium showed reduced immunogenicity but comparable collagen composition and physical characteristics of the pericardium from wild-type pigs.
29631050	14	83	theme	pericardium	2597:2607	arg1	composition					2549:2559	comparable collagen composition	2529:2559	comparable collagen composition	2529:2559	The genetically modified porcine pericardium showed reduced immunogenicity but comparable collagen composition and physical characteristics of the pericardium from wild-type pigs.
29631050	12	84	theme	bovine	2006:2011	arg1	tissues					2013:2019	glutaraldehyde-fixed porcine or bovine tissues	1974:2019	tissues	2013:2019	Bioprosthetic heart valves (BHVs) made from glutaraldehyde-fixed porcine or bovine tissues are widely used in clinics but exhibit age-dependent structural valve degeneration (SVD) which is associated with the immune response against BHVs.
29631050	13	85	from	targeting	2418:2426	arg1	study					2443:2447	the present study	2431:2447	the present study	2431:2447	Three major xenoantigens present on commercial BHVs, Galactosea α1,3 galactose (αGal), N-glycolylneuraminic acid (Neu5Gc) and glycan products of β-1,4-N-acetyl-galactosaminyl transferase 2 (β4GalNT2) are eliminated through CRISPR/Cas9 mediated gene targeting in the present study.
29631050	0	86	theme	glycan	100:105	arg1	GGTA1/β4GalNT2/CMAH					117:135	GGTA1/β4GalNT2/CMAH	117:135	GGTA1/β4GalNT2/CMAH	117:135	Reducing immunoreactivity of porcine bioprosthetic heart valves by genetically-deleting three major glycan antigens, GGTA1/β4GalNT2/CMAH.
29631050	0	86	theme	glycan	100:105	arg1	antigens					107:114	three major glycan antigens	88:114	three major glycan antigens	88:114	Reducing immunoreactivity of porcine bioprosthetic heart valves by genetically-deleting three major glycan antigens, GGTA1/β4GalNT2/CMAH.
29631050	5	87	theme	human	900:904	arg1	binding					914:920	human IgM/IgG binding	900:920	human IgM/IgG binding	900:920	Moreover, valves from αGal/Neu5Gc-deficient pigs could further reduce human IgM/IgG binding when compared to BHV from αGal-knockout pigs.
29631050	9	88	theme	porcine	1712:1718	arg1	pericardium					1720:1730	porcine pericardium	1712:1730	porcine pericardium	1712:1730	Moreover, the analysis of collagen composition and physical characteristics of porcine pericardium from the TKO pigs indicated that elimination of the three xenoantigens had no significant impact on the physical proprieties of porcine pericardium.
29631050	8	89	theme	IgG/IgM	1441:1447	arg1	binding					1449:1455	human IgG/IgM binding	1435:1455	human IgG/IgM binding to pericardium	1435:1470	Our results showed that the expression of αGal, Neu5G and Sd(a) on TKO pigs was negative, and that human IgG/IgM binding to pericardium was minimal.
29631050	13	90	theme	mediated	2404:2411	arg1	targeting					2418:2426	CRISPR/Cas9 mediated gene targeting	2392:2426	CRISPR/Cas9 mediated gene targeting in the present study	2392:2447	Three major xenoantigens present on commercial BHVs, Galactosea α1,3 galactose (αGal), N-glycolylneuraminic acid (Neu5Gc) and glycan products of β-1,4-N-acetyl-galactosaminyl transferase 2 (β4GalNT2) are eliminated through CRISPR/Cas9 mediated gene targeting in the present study.
29631050	14	91	theme	physical	2565:2572	arg1	characteristics					2574:2588	physical characteristics	2565:2588	physical characteristics	2565:2588	The genetically modified porcine pericardium showed reduced immunogenicity but comparable collagen composition and physical characteristics of the pericardium from wild-type pigs.
29631050	15	92	from	pigs	2744:2747	arg1	BHVs					2724:2727	currently available BHVs	2704:2727	currently available BHVs from wild-type pigs	2704:2747	Our data suggested that BHVs from TKO pigs is a promising alternative for currently available BHVs from wild-type pigs.
29631050	7	93	theme	triple	1158:1163	arg1	pigs					1185:1188	GGTA1, CMAH, and β4GalNT2 triple gene-knockout (TKO) pigs	1132:1188	GGTA1, CMAH, and β4GalNT2 triple gene-knockout (TKO) pigs	1132:1188	To explore whether tissue from GGTA1, CMAH, and β4GalNT2 triple gene-knockout (TKO) pigs would further minimize human antibody binding to porcine pericardium, TKO pigs were successfully produced by CRISPR/Cas9 mediated gene targeting.
29631050	3	94	from	epitopes	406:413	arg1	BHVs					426:429	porcine BHVs	418:429	porcine BHVs	418:429	Two well-characterized epitopes on porcine BHVs have been implicated in SVD, including galactose-α1,3-galactose (αGal) and N-glycolylneuraminic acid (Neu5Gc) whose synthesis are catalyzed by α(1,3) galactosyltransferase (encoded by the GGTA1 gene) and CMP-Neu5Ac hydroxylase (encoded by the CMAH gene), respectively.
29631050	4	95	from	pigs	749:752	arg1	BHV					726:728	BHV	726:728	BHV from αGal-knockout pigs	726:752	It has been reported that BHV from αGal-knockout pigs are associated with a significantly reduced immune response by human serum.
29631050	7	96	theme	CRISPR/Cas9	1299:1309	arg1	targeting					1325:1333	CRISPR/Cas9 mediated gene targeting	1299:1333	CRISPR/Cas9 mediated gene targeting	1299:1333	To explore whether tissue from GGTA1, CMAH, and β4GalNT2 triple gene-knockout (TKO) pigs would further minimize human antibody binding to porcine pericardium, TKO pigs were successfully produced by CRISPR/Cas9 mediated gene targeting.
29631050	15	97	theme	TKO	2664:2666	arg1	pigs					2668:2671	TKO pigs	2664:2671	TKO pigs	2664:2671	Our data suggested that BHVs from TKO pigs is a promising alternative for currently available BHVs from wild-type pigs.
29631050	3	98	theme	porcine	418:424	arg1	BHVs					426:429	porcine BHVs	418:429	porcine BHVs	418:429	Two well-characterized epitopes on porcine BHVs have been implicated in SVD, including galactose-α1,3-galactose (αGal) and N-glycolylneuraminic acid (Neu5Gc) whose synthesis are catalyzed by α(1,3) galactosyltransferase (encoded by the GGTA1 gene) and CMP-Neu5Ac hydroxylase (encoded by the CMAH gene), respectively.
29631050	11	99	theme	valve	1847:1851	arg1	replacement					1853:1863	SIGNIFICANCE Surgical heart valve replacement	1819:1863	SIGNIFICANCE Surgical heart valve replacement	1819:1863	STATEMENT OF SIGNIFICANCE Surgical heart valve replacement is an established lifesaving treatment for diseased heart valve.
29631050	0	100	theme	porcine	29:35	arg1	valves					57:62	porcine bioprosthetic heart valves	29:62	porcine bioprosthetic heart valves	29:62	Reducing immunoreactivity of porcine bioprosthetic heart valves by genetically-deleting three major glycan antigens, GGTA1/β4GalNT2/CMAH.
29631050	13	101	theme	β-1,4-N-acetyl-galactosaminyl	2314:2342	arg1	transferase					2344:2354	β-1,4-N-acetyl-galactosaminyl transferase 2	2314:2356	β-1,4-N-acetyl-galactosaminyl transferase 2 (β4GalNT2)	2314:2367	Three major xenoantigens present on commercial BHVs, Galactosea α1,3 galactose (αGal), N-glycolylneuraminic acid (Neu5Gc) and glycan products of β-1,4-N-acetyl-galactosaminyl transferase 2 (β4GalNT2) are eliminated through CRISPR/Cas9 mediated gene targeting in the present study.
29631050	13	101	theme	β-1,4-N-acetyl-galactosaminyl	2314:2342	arg1	β4GalNT2					2359:2366	β4GalNT2	2359:2366	β4GalNT2	2359:2366	Three major xenoantigens present on commercial BHVs, Galactosea α1,3 galactose (αGal), N-glycolylneuraminic acid (Neu5Gc) and glycan products of β-1,4-N-acetyl-galactosaminyl transferase 2 (β4GalNT2) are eliminated through CRISPR/Cas9 mediated gene targeting in the present study.
29631050	5	102	theme	αGal-knockout	948:960	arg1	pigs					962:965	αGal-knockout pigs	948:965	αGal-knockout pigs	948:965	Moreover, valves from αGal/Neu5Gc-deficient pigs could further reduce human IgM/IgG binding when compared to BHV from αGal-knockout pigs.
29631050	9	103	from	characteristics	1545:1559	arg1	pigs					1597:1600	the TKO pigs	1589:1600	the TKO pigs	1589:1600	Moreover, the analysis of collagen composition and physical characteristics of porcine pericardium from the TKO pigs indicated that elimination of the three xenoantigens had no significant impact on the physical proprieties of porcine pericardium.
29631050	13	104	theme	major	2175:2179	arg1	xenoantigens					2181:2192	Three major xenoantigens	2169:2192	Three major xenoantigens present on commercial BHVs, Galactosea α1,3 galactose (αGal), N-glycolylneuraminic acid (Neu5Gc) and glycan products of β-1,4-N-acetyl-galactosaminyl transferase 2 (β4GalNT2)	2169:2367	Three major xenoantigens present on commercial BHVs, Galactosea α1,3 galactose (αGal), N-glycolylneuraminic acid (Neu5Gc) and glycan products of β-1,4-N-acetyl-galactosaminyl transferase 2 (β4GalNT2) are eliminated through CRISPR/Cas9 mediated gene targeting in the present study.
29631050	7	105	theme	GGTA1	1132:1136	arg1	pigs					1185:1188	GGTA1, CMAH, and β4GalNT2 triple gene-knockout (TKO) pigs	1132:1188	GGTA1, CMAH, and β4GalNT2 triple gene-knockout (TKO) pigs	1132:1188	To explore whether tissue from GGTA1, CMAH, and β4GalNT2 triple gene-knockout (TKO) pigs would further minimize human antibody binding to porcine pericardium, TKO pigs were successfully produced by CRISPR/Cas9 mediated gene targeting.
29631050	13	106	theme	commercial	2205:2214	arg1	BHVs					2216:2219	commercial BHVs	2205:2219	commercial BHVs	2205:2219	Three major xenoantigens present on commercial BHVs, Galactosea α1,3 galactose (αGal), N-glycolylneuraminic acid (Neu5Gc) and glycan products of β-1,4-N-acetyl-galactosaminyl transferase 2 (β4GalNT2) are eliminated through CRISPR/Cas9 mediated gene targeting in the present study.
29631050	14	107	theme	collagen	2540:2547	arg1	composition					2549:2559	comparable collagen composition	2529:2559	comparable collagen composition	2529:2559	The genetically modified porcine pericardium showed reduced immunogenicity but comparable collagen composition and physical characteristics of the pericardium from wild-type pigs.
29631050	9	108	theme	composition	1520:1530	arg1	analysis					1499:1506	the analysis	1495:1506	the analysis of collagen composition and physical characteristics of porcine pericardium from the TKO pigs	1495:1600	Moreover, the analysis of collagen composition and physical characteristics of porcine pericardium from the TKO pigs indicated that elimination of the three xenoantigens had no significant impact on the physical proprieties of porcine pericardium.
29631050	7	109	theme	TKO	1260:1262	arg1	pigs					1264:1267	TKO pigs	1260:1267	TKO pigs	1260:1267	To explore whether tissue from GGTA1, CMAH, and β4GalNT2 triple gene-knockout (TKO) pigs would further minimize human antibody binding to porcine pericardium, TKO pigs were successfully produced by CRISPR/Cas9 mediated gene targeting.
29631050	3	110	theme	CMP-Neu5Ac	635:644	arg1	hydroxylase					646:656	CMP-Neu5Ac hydroxylase	635:656	CMP-Neu5Ac hydroxylase (encoded by the CMAH gene)	635:683	Two well-characterized epitopes on porcine BHVs have been implicated in SVD, including galactose-α1,3-galactose (αGal) and N-glycolylneuraminic acid (Neu5Gc) whose synthesis are catalyzed by α(1,3) galactosyltransferase (encoded by the GGTA1 gene) and CMP-Neu5Ac hydroxylase (encoded by the CMAH gene), respectively.
29631050	13	111	attach	present	2194:2200	arg1	BHVs					2216:2219	commercial BHVs	2205:2219	commercial BHVs	2205:2219	Three major xenoantigens present on commercial BHVs, Galactosea α1,3 galactose (αGal), N-glycolylneuraminic acid (Neu5Gc) and glycan products of β-1,4-N-acetyl-galactosaminyl transferase 2 (β4GalNT2) are eliminated through CRISPR/Cas9 mediated gene targeting in the present study.
29631050	13	111	attach	present	2194:2200	arg1	acid					2277:2280	N-glycolylneuraminic acid	2256:2280	N-glycolylneuraminic acid (Neu5Gc)	2256:2289	Three major xenoantigens present on commercial BHVs, Galactosea α1,3 galactose (αGal), N-glycolylneuraminic acid (Neu5Gc) and glycan products of β-1,4-N-acetyl-galactosaminyl transferase 2 (β4GalNT2) are eliminated through CRISPR/Cas9 mediated gene targeting in the present study.
29631050	13	111	attach	present	2194:2200	arg1	products					2302:2309	glycan products	2295:2309	glycan products of β-1,4-N-acetyl-galactosaminyl transferase 2 (β4GalNT2)	2295:2367	Three major xenoantigens present on commercial BHVs, Galactosea α1,3 galactose (αGal), N-glycolylneuraminic acid (Neu5Gc) and glycan products of β-1,4-N-acetyl-galactosaminyl transferase 2 (β4GalNT2) are eliminated through CRISPR/Cas9 mediated gene targeting in the present study.
29631050	13	111	attach	present	2194:2200	arg1	galactose					2238:2246	Galactosea α1,3 galactose	2222:2246	Galactosea α1,3 galactose (αGal)	2222:2253	Three major xenoantigens present on commercial BHVs, Galactosea α1,3 galactose (αGal), N-glycolylneuraminic acid (Neu5Gc) and glycan products of β-1,4-N-acetyl-galactosaminyl transferase 2 (β4GalNT2) are eliminated through CRISPR/Cas9 mediated gene targeting in the present study.
29631050	13	111	attach	present	2194:2200	arg1	αGal					2249:2252	αGal	2249:2252	αGal	2249:2252	Three major xenoantigens present on commercial BHVs, Galactosea α1,3 galactose (αGal), N-glycolylneuraminic acid (Neu5Gc) and glycan products of β-1,4-N-acetyl-galactosaminyl transferase 2 (β4GalNT2) are eliminated through CRISPR/Cas9 mediated gene targeting in the present study.
29631050	13	111	attach	present	2194:2200	arg2	xenoantigens					2181:2192	Three major xenoantigens	2169:2192	Three major xenoantigens present on commercial BHVs, Galactosea α1,3 galactose (αGal), N-glycolylneuraminic acid (Neu5Gc) and glycan products of β-1,4-N-acetyl-galactosaminyl transferase 2 (β4GalNT2)	2169:2367	Three major xenoantigens present on commercial BHVs, Galactosea α1,3 galactose (αGal), N-glycolylneuraminic acid (Neu5Gc) and glycan products of β-1,4-N-acetyl-galactosaminyl transferase 2 (β4GalNT2) are eliminated through CRISPR/Cas9 mediated gene targeting in the present study.
29631050	13	111	attach	present	2194:2200	arg1	Neu5Gc					2283:2288	Neu5Gc	2283:2288	Neu5Gc	2283:2288	Three major xenoantigens present on commercial BHVs, Galactosea α1,3 galactose (αGal), N-glycolylneuraminic acid (Neu5Gc) and glycan products of β-1,4-N-acetyl-galactosaminyl transferase 2 (β4GalNT2) are eliminated through CRISPR/Cas9 mediated gene targeting in the present study.
29631050	11	112	theme	established	1871:1881	arg1	STATEMENT					1806:1814	STATEMENT	1806:1814	STATEMENT OF SIGNIFICANCE Surgical heart valve replacement	1806:1863	STATEMENT OF SIGNIFICANCE Surgical heart valve replacement is an established lifesaving treatment for diseased heart valve.
29631050	11	112	theme	established	1871:1881	arg1	treatment					1894:1902	an established lifesaving treatment	1868:1902	an established lifesaving treatment for diseased heart valve	1868:1927	STATEMENT OF SIGNIFICANCE Surgical heart valve replacement is an established lifesaving treatment for diseased heart valve.
29631050	15	113	theme	available	2714:2722	arg1	BHVs					2724:2727	currently available BHVs	2704:2727	currently available BHVs from wild-type pigs	2704:2747	Our data suggested that BHVs from TKO pigs is a promising alternative for currently available BHVs from wild-type pigs.
29631050	9	114	theme	physical	1536:1543	arg1	characteristics					1545:1559	physical characteristics	1536:1559	physical characteristics	1536:1559	Moreover, the analysis of collagen composition and physical characteristics of porcine pericardium from the TKO pigs indicated that elimination of the three xenoantigens had no significant impact on the physical proprieties of porcine pericardium.
29631050	3	115	theme	CMAH	674:677	arg1	gene					679:682	the CMAH gene	670:682	the CMAH gene	670:682	Two well-characterized epitopes on porcine BHVs have been implicated in SVD, including galactose-α1,3-galactose (αGal) and N-glycolylneuraminic acid (Neu5Gc) whose synthesis are catalyzed by α(1,3) galactosyltransferase (encoded by the GGTA1 gene) and CMP-Neu5Ac hydroxylase (encoded by the CMAH gene), respectively.
29631050	8	116	theme	αGal	1378:1381	arg1	expression					1364:1373	the expression	1360:1373	the expression of αGal, Neu5G and Sd(a) on TKO pigs	1360:1410	Our results showed that the expression of αGal, Neu5G and Sd(a) on TKO pigs was negative, and that human IgG/IgM binding to pericardium was minimal.
29631050	8	116	theme	αGal	1378:1381	arg1	negative					1416:1423	negative	1416:1423	negative	1416:1423	Our results showed that the expression of αGal, Neu5G and Sd(a) on TKO pigs was negative, and that human IgG/IgM binding to pericardium was minimal.
29631050	9	117	contain	had	1655:1657	arg1	elimination					1617:1627	elimination	1617:1627	elimination of the three xenoantigens	1617:1653	Moreover, the analysis of collagen composition and physical characteristics of porcine pericardium from the TKO pigs indicated that elimination of the three xenoantigens had no significant impact on the physical proprieties of porcine pericardium.
29631050	9	117	contain	had	1655:1657	arg2	impact					1674:1679	no significant impact	1659:1679	no significant impact	1659:1679	Moreover, the analysis of collagen composition and physical characteristics of porcine pericardium from the TKO pigs indicated that elimination of the three xenoantigens had no significant impact on the physical proprieties of porcine pericardium.
29631050	4	118	theme	reduced	790:796	arg1	response					805:812	a significantly reduced immune response	774:812	a significantly reduced immune response by human serum	774:827	It has been reported that BHV from αGal-knockout pigs are associated with a significantly reduced immune response by human serum.
29631050	14	119	theme	reduced	2502:2508	arg1	immunogenicity					2510:2523	immunogenicity	2510:2523	immunogenicity	2510:2523	The genetically modified porcine pericardium showed reduced immunogenicity but comparable collagen composition and physical characteristics of the pericardium from wild-type pigs.
29631050	10	120	theme	TKO	1763:1765	arg1	source					1790:1795	an ideal source	1781:1795	an ideal source of BHVs	1781:1803	Our results demonstrated that TKO pigs would be an ideal source of BHVs.
29631050	10	120	theme	TKO	1763:1765	arg1	pigs					1767:1770	TKO pigs	1763:1770	TKO pigs	1763:1770	Our results demonstrated that TKO pigs would be an ideal source of BHVs.
29631050	8	121	theme	Neu5G	1384:1388	arg1	expression					1364:1373	the expression	1360:1373	the expression of αGal, Neu5G and Sd(a) on TKO pigs	1360:1410	Our results showed that the expression of αGal, Neu5G and Sd(a) on TKO pigs was negative, and that human IgG/IgM binding to pericardium was minimal.
29631050	8	121	theme	Neu5G	1384:1388	arg1	negative					1416:1423	negative	1416:1423	negative	1416:1423	Our results showed that the expression of αGal, Neu5G and Sd(a) on TKO pigs was negative, and that human IgG/IgM binding to pericardium was minimal.
29631050	3	122	theme	N-glycolylneuraminic	506:525	arg1	acid					527:530	N-glycolylneuraminic acid	506:530	N-glycolylneuraminic acid (Neu5Gc)	506:539	Two well-characterized epitopes on porcine BHVs have been implicated in SVD, including galactose-α1,3-galactose (αGal) and N-glycolylneuraminic acid (Neu5Gc) whose synthesis are catalyzed by α(1,3) galactosyltransferase (encoded by the GGTA1 gene) and CMP-Neu5Ac hydroxylase (encoded by the CMAH gene), respectively.
29631050	3	122	theme	N-glycolylneuraminic	506:525	arg1	Neu5Gc					533:538	Neu5Gc	533:538	Neu5Gc	533:538	Two well-characterized epitopes on porcine BHVs have been implicated in SVD, including galactose-α1,3-galactose (αGal) and N-glycolylneuraminic acid (Neu5Gc) whose synthesis are catalyzed by α(1,3) galactosyltransferase (encoded by the GGTA1 gene) and CMP-Neu5Ac hydroxylase (encoded by the CMAH gene), respectively.
29631050	12	123	theme	glutaraldehyde-fixed	1974:1993	arg1	porcine					1995:2001	glutaraldehyde-fixed porcine or bovine tissues	1974:2019	porcine	1995:2001	Bioprosthetic heart valves (BHVs) made from glutaraldehyde-fixed porcine or bovine tissues are widely used in clinics but exhibit age-dependent structural valve degeneration (SVD) which is associated with the immune response against BHVs.
29631050	9	124	theme	pericardium	1572:1582	arg1	composition					1520:1530	collagen composition	1511:1530	collagen composition	1511:1530	Moreover, the analysis of collagen composition and physical characteristics of porcine pericardium from the TKO pigs indicated that elimination of the three xenoantigens had no significant impact on the physical proprieties of porcine pericardium.
29631050	9	124	theme	pericardium	1572:1582	arg1	characteristics					1545:1559	physical characteristics	1536:1559	physical characteristics	1536:1559	Moreover, the analysis of collagen composition and physical characteristics of porcine pericardium from the TKO pigs indicated that elimination of the three xenoantigens had no significant impact on the physical proprieties of porcine pericardium.
29631050	7	125	theme	antibody	1219:1226	arg1	binding					1228:1234	human antibody binding	1213:1234	human antibody binding to porcine pericardium	1213:1257	To explore whether tissue from GGTA1, CMAH, and β4GalNT2 triple gene-knockout (TKO) pigs would further minimize human antibody binding to porcine pericardium, TKO pigs were successfully produced by CRISPR/Cas9 mediated gene targeting.
29631050	8	126	theme	Sd	1394:1395	arg1	expression					1364:1373	the expression	1360:1373	the expression of αGal, Neu5G and Sd(a) on TKO pigs	1360:1410	Our results showed that the expression of αGal, Neu5G and Sd(a) on TKO pigs was negative, and that human IgG/IgM binding to pericardium was minimal.
29631050	8	126	theme	Sd	1394:1395	arg1	negative					1416:1423	negative	1416:1423	negative	1416:1423	Our results showed that the expression of αGal, Neu5G and Sd(a) on TKO pigs was negative, and that human IgG/IgM binding to pericardium was minimal.
29631050	11	127	theme	Surgical	1832:1839	arg1	replacement					1853:1863	SIGNIFICANCE Surgical heart valve replacement	1819:1863	SIGNIFICANCE Surgical heart valve replacement	1819:1863	STATEMENT OF SIGNIFICANCE Surgical heart valve replacement is an established lifesaving treatment for diseased heart valve.
29575162	4	0	theme	LC	1045:1046	arg1	separation					1048:1057	LC separation	1045:1057	LC separation	1045:1057	N-linked glycans are first released from glycoproteins by enzymatic digestion, then labeled with fluorescence dyes for subsequent CE or LC separation, and LIF or MS detection.
29575162	4	1	link	N-linked	909:916	arg1	glycans					918:924	N-linked glycans	909:924	N-linked glycans	909:924	N-linked glycans are first released from glycoproteins by enzymatic digestion, then labeled with fluorescence dyes for subsequent CE or LC separation, and LIF or MS detection.
29575162	8	2	from	quantitation	1826:1837	arg1	proteins					1905:1912	recombinant therapeutic proteins	1881:1912	recombinant therapeutic proteins	1881:1912	CONCLUSIONS This on-line CE/LIF/MS method using Teal™ fluorescent dye and electrokinetic pump-based nanospray sheath liquid CE/MS coupling technology holds promise for on-line quantitation and identification of N-linked glycans on recombinant therapeutic proteins.
29575162	1	3	theme	Fc-fusion	308:316	arg1	proteins					318:325	Fc-fusion proteins	308:325	Fc-fusion proteins	308:325	RATIONALE N-linked glycan analysis of recombinant therapeutic proteins, such as monoclonal antibodies, Fc-fusion proteins, and antibody-drug conjugates, provides valuable information regarding protein therapeutics glycosylation profile.
29575162	7	4	theme	ion	1601:1603	arg1	source					1605:1610	this versatile CE/MS ion source	1580:1610	this versatile CE/MS ion source	1580:1610	Results show that on-line CE/LIF/MS analysis can be readily achieved using this versatile CE/MS ion source on common CE/MS instrument platforms.
29575162	6	5	theme	glycans	1496:1502	arg1	analysis					1454:1461	the negative ion mode CE/MS analysis	1426:1461	the negative ion mode CE/MS analysis of fluorescence-labeled N-linked glycans	1426:1502	RESULTS Electrophoresis running buffer systems using ammonium acetate and ammonium hydroxide were developed for the negative ion mode CE/MS analysis of fluorescence-labeled N-linked glycans.
29575162	6	6	theme	fluorescence-labeled	1466:1485	arg1	glycans					1496:1502	fluorescence-labeled N-linked glycans	1466:1502	fluorescence-labeled N-linked glycans	1466:1502	RESULTS Electrophoresis running buffer systems using ammonium acetate and ammonium hydroxide were developed for the negative ion mode CE/MS analysis of fluorescence-labeled N-linked glycans.
29575162	1	7	theme	glycan	224:229	arg1	analysis					231:238	RATIONALE N-linked glycan analysis	205:238	RATIONALE N-linked glycan analysis of recombinant therapeutic proteins, such as monoclonal antibodies, Fc-fusion proteins, and antibody-drug conjugates,	205:356	RATIONALE N-linked glycan analysis of recombinant therapeutic proteins, such as monoclonal antibodies, Fc-fusion proteins, and antibody-drug conjugates, provides valuable information regarding protein therapeutics glycosylation profile.
29575162	6	8	theme	ammonium	1367:1374	arg1	acetate					1376:1382	ammonium acetate	1367:1382	ammonium acetate	1367:1382	RESULTS Electrophoresis running buffer systems using ammonium acetate and ammonium hydroxide were developed for the negative ion mode CE/MS analysis of fluorescence-labeled N-linked glycans.
29575162	5	9	dep	dye	1189:1191	arg1	source					1306:1311	ion source	1302:1311	ion source	1302:1311	Here we present an on-line CE/LIF/MS N-glycan analysis workflow that incorporates the fluorescent Teal™ dye and an electrokinetic pump-based nanospray sheath liquid capillary electrophoresis/mass spectrometry (CE/MS) ion source.
29575162	8	10	from	identification	1843:1856	arg1	proteins					1905:1912	recombinant therapeutic proteins	1881:1912	recombinant therapeutic proteins	1881:1912	CONCLUSIONS This on-line CE/LIF/MS method using Teal™ fluorescent dye and electrokinetic pump-based nanospray sheath liquid CE/MS coupling technology holds promise for on-line quantitation and identification of N-linked glycans on recombinant therapeutic proteins.
29575162	7	11	theme	CE/MS	1622:1626	arg1	platforms					1639:1647	common CE/MS instrument platforms	1615:1647	common CE/MS instrument platforms	1615:1647	Results show that on-line CE/LIF/MS analysis can be readily achieved using this versatile CE/MS ion source on common CE/MS instrument platforms.
29575162	0	12	theme	sheath	158:163	arg1	source					197:202	an electrokinetic sheath liquid pump-based nanospray ion source	140:202	an electrokinetic sheath liquid pump-based nanospray ion source	140:202	On-line capillary electrophoresis/laser-induced fluorescence/mass spectrometry analysis of glycans labeled with Teal™ fluorescent dye using an electrokinetic sheath liquid pump-based nanospray ion source.
29575162	5	13	theme	capillary	1250:1258	arg1	CE/MS					1295:1299	CE/MS	1295:1299	CE/MS	1295:1299	Here we present an on-line CE/LIF/MS N-glycan analysis workflow that incorporates the fluorescent Teal™ dye and an electrokinetic pump-based nanospray sheath liquid capillary electrophoresis/mass spectrometry (CE/MS) ion source.
29575162	5	13	theme	capillary	1250:1258	arg1	spectrometry					1281:1292	an electrokinetic pump-based nanospray sheath liquid capillary electrophoresis/mass spectrometry	1197:1292	an electrokinetic pump-based nanospray sheath liquid capillary electrophoresis/mass spectrometry (CE/MS)	1197:1300	Here we present an on-line CE/LIF/MS N-glycan analysis workflow that incorporates the fluorescent Teal™ dye and an electrokinetic pump-based nanospray sheath liquid capillary electrophoresis/mass spectrometry (CE/MS) ion source.
29575162	1	14	theme	therapeutic	255:265	arg1	conjugates					346:355	antibody-drug conjugates	332:355	antibody-drug conjugates	332:355	RATIONALE N-linked glycan analysis of recombinant therapeutic proteins, such as monoclonal antibodies, Fc-fusion proteins, and antibody-drug conjugates, provides valuable information regarding protein therapeutics glycosylation profile.
29575162	1	14	theme	therapeutic	255:265	arg1	proteins					318:325	Fc-fusion proteins	308:325	Fc-fusion proteins	308:325	RATIONALE N-linked glycan analysis of recombinant therapeutic proteins, such as monoclonal antibodies, Fc-fusion proteins, and antibody-drug conjugates, provides valuable information regarding protein therapeutics glycosylation profile.
29575162	1	14	theme	therapeutic	255:265	arg1	antibodies					296:305	monoclonal antibodies	285:305	monoclonal antibodies	285:305	RATIONALE N-linked glycan analysis of recombinant therapeutic proteins, such as monoclonal antibodies, Fc-fusion proteins, and antibody-drug conjugates, provides valuable information regarding protein therapeutics glycosylation profile.
29575162	1	14	theme	therapeutic	255:265	arg1	proteins					267:274	recombinant therapeutic proteins	243:274	recombinant therapeutic proteins	243:274	RATIONALE N-linked glycan analysis of recombinant therapeutic proteins, such as monoclonal antibodies, Fc-fusion proteins, and antibody-drug conjugates, provides valuable information regarding protein therapeutics glycosylation profile.
29575162	6	15	theme	CE/MS	1448:1452	arg1	analysis					1454:1461	the negative ion mode CE/MS analysis	1426:1461	the negative ion mode CE/MS analysis of fluorescence-labeled N-linked glycans	1426:1502	RESULTS Electrophoresis running buffer systems using ammonium acetate and ammonium hydroxide were developed for the negative ion mode CE/MS analysis of fluorescence-labeled N-linked glycans.
29575162	0	16	theme	pump-based	172:181	arg1	source					197:202	an electrokinetic sheath liquid pump-based nanospray ion source	140:202	an electrokinetic sheath liquid pump-based nanospray ion source	140:202	On-line capillary electrophoresis/laser-induced fluorescence/mass spectrometry analysis of glycans labeled with Teal™ fluorescent dye using an electrokinetic sheath liquid pump-based nanospray ion source.
29575162	3	17	theme	capillary	718:726	arg1	technologies					895:906	capillary electrophoresis/laser-induced fluorescence (CE/LIF), liquid chromatography/fluorescence (LC/FLR), and liquid chromatography/fluorescence/mass spectrometry (LC/FLR/MS) technologies	718:906	capillary electrophoresis/laser-induced fluorescence (CE/LIF), liquid chromatography/fluorescence (LC/FLR), and liquid chromatography/fluorescence/mass spectrometry (LC/FLR/MS) technologies	718:906	METHODS Currently, such analyses are mainly carried out using capillary electrophoresis/laser-induced fluorescence (CE/LIF), liquid chromatography/fluorescence (LC/FLR), and liquid chromatography/fluorescence/mass spectrometry (LC/FLR/MS) technologies.
29575162	8	18	theme	Teal™	1698:1702	arg1	dye					1716:1718	Teal™ fluorescent dye	1698:1718	Teal™ fluorescent dye	1698:1718	CONCLUSIONS This on-line CE/LIF/MS method using Teal™ fluorescent dye and electrokinetic pump-based nanospray sheath liquid CE/MS coupling technology holds promise for on-line quantitation and identification of N-linked glycans on recombinant therapeutic proteins.
29575162	6	19	theme	ion	1439:1441	arg1	mode					1443:1446	the negative ion mode	1426:1446	the negative ion mode CE/MS analysis of fluorescence-labeled N-linked glycans	1426:1502	RESULTS Electrophoresis running buffer systems using ammonium acetate and ammonium hydroxide were developed for the negative ion mode CE/MS analysis of fluorescence-labeled N-linked glycans.
29575162	0	20	theme	ion	193:195	arg1	source					197:202	an electrokinetic sheath liquid pump-based nanospray ion source	140:202	an electrokinetic sheath liquid pump-based nanospray ion source	140:202	On-line capillary electrophoresis/laser-induced fluorescence/mass spectrometry analysis of glycans labeled with Teal™ fluorescent dye using an electrokinetic sheath liquid pump-based nanospray ion source.
29575162	5	21	theme	pump-based	1215:1224	arg1	CE/MS					1295:1299	CE/MS	1295:1299	CE/MS	1295:1299	Here we present an on-line CE/LIF/MS N-glycan analysis workflow that incorporates the fluorescent Teal™ dye and an electrokinetic pump-based nanospray sheath liquid capillary electrophoresis/mass spectrometry (CE/MS) ion source.
29575162	5	21	theme	pump-based	1215:1224	arg1	spectrometry					1281:1292	an electrokinetic pump-based nanospray sheath liquid capillary electrophoresis/mass spectrometry	1197:1292	an electrokinetic pump-based nanospray sheath liquid capillary electrophoresis/mass spectrometry (CE/MS)	1197:1300	Here we present an on-line CE/LIF/MS N-glycan analysis workflow that incorporates the fluorescent Teal™ dye and an electrokinetic pump-based nanospray sheath liquid capillary electrophoresis/mass spectrometry (CE/MS) ion source.
29575162	3	22	dep	METHODS	656:662	arg1	carried					700:706	carried	700:706	are mainly carried out using capillary electrophoresis/laser-induced fluorescence (CE/LIF), liquid chromatography/fluorescence (LC/FLR), and liquid chromatography/fluorescence/mass spectrometry (LC/FLR/MS) technologies	689:906	METHODS Currently, such analyses are mainly carried out using capillary electrophoresis/laser-induced fluorescence (CE/LIF), liquid chromatography/fluorescence (LC/FLR), and liquid chromatography/fluorescence/mass spectrometry (LC/FLR/MS) technologies.
29575162	4	23	theme	MS	1071:1072	arg1	detection					1074:1082	MS detection	1071:1082	MS detection	1071:1082	N-linked glycans are first released from glycoproteins by enzymatic digestion, then labeled with fluorescence dyes for subsequent CE or LC separation, and LIF or MS detection.
29575162	7	24	theme	versatile	1585:1593	arg1	source					1605:1610	this versatile CE/MS ion source	1580:1610	this versatile CE/MS ion source	1580:1610	Results show that on-line CE/LIF/MS analysis can be readily achieved using this versatile CE/MS ion source on common CE/MS instrument platforms.
29575162	5	25	theme	sheath	1236:1241	arg1	CE/MS					1295:1299	CE/MS	1295:1299	CE/MS	1295:1299	Here we present an on-line CE/LIF/MS N-glycan analysis workflow that incorporates the fluorescent Teal™ dye and an electrokinetic pump-based nanospray sheath liquid capillary electrophoresis/mass spectrometry (CE/MS) ion source.
29575162	5	25	theme	sheath	1236:1241	arg1	spectrometry					1281:1292	an electrokinetic pump-based nanospray sheath liquid capillary electrophoresis/mass spectrometry	1197:1292	an electrokinetic pump-based nanospray sheath liquid capillary electrophoresis/mass spectrometry (CE/MS)	1197:1300	Here we present an on-line CE/LIF/MS N-glycan analysis workflow that incorporates the fluorescent Teal™ dye and an electrokinetic pump-based nanospray sheath liquid capillary electrophoresis/mass spectrometry (CE/MS) ion source.
29575162	8	26	dep	CONCLUSIONS	1650:1660	arg1	holds					1800:1804	holds	1800:1804	holds promise for on-line quantitation and identification of N-linked glycans on recombinant therapeutic proteins	1800:1912	CONCLUSIONS This on-line CE/LIF/MS method using Teal™ fluorescent dye and electrokinetic pump-based nanospray sheath liquid CE/MS coupling technology holds promise for on-line quantitation and identification of N-linked glycans on recombinant therapeutic proteins.
29575162	8	27	theme	electrokinetic	1724:1737	arg1	technology					1789:1798	electrokinetic pump-based nanospray sheath liquid CE/MS coupling technology	1724:1798	electrokinetic pump-based nanospray sheath liquid CE/MS coupling technology	1724:1798	CONCLUSIONS This on-line CE/LIF/MS method using Teal™ fluorescent dye and electrokinetic pump-based nanospray sheath liquid CE/MS coupling technology holds promise for on-line quantitation and identification of N-linked glycans on recombinant therapeutic proteins.
29575162	1	28	theme	protein	398:404	arg1	therapeutics					406:417	protein therapeutics	398:417	protein therapeutics	398:417	RATIONALE N-linked glycan analysis of recombinant therapeutic proteins, such as monoclonal antibodies, Fc-fusion proteins, and antibody-drug conjugates, provides valuable information regarding protein therapeutics glycosylation profile.
29575162	3	29	theme	fluorescence	758:769	arg1	technologies					895:906	capillary electrophoresis/laser-induced fluorescence (CE/LIF), liquid chromatography/fluorescence (LC/FLR), and liquid chromatography/fluorescence/mass spectrometry (LC/FLR/MS) technologies	718:906	capillary electrophoresis/laser-induced fluorescence (CE/LIF), liquid chromatography/fluorescence (LC/FLR), and liquid chromatography/fluorescence/mass spectrometry (LC/FLR/MS) technologies	718:906	METHODS Currently, such analyses are mainly carried out using capillary electrophoresis/laser-induced fluorescence (CE/LIF), liquid chromatography/fluorescence (LC/FLR), and liquid chromatography/fluorescence/mass spectrometry (LC/FLR/MS) technologies.
29575162	8	30	theme	nanospray	1750:1758	arg1	technology					1789:1798	electrokinetic pump-based nanospray sheath liquid CE/MS coupling technology	1724:1798	electrokinetic pump-based nanospray sheath liquid CE/MS coupling technology	1724:1798	CONCLUSIONS This on-line CE/LIF/MS method using Teal™ fluorescent dye and electrokinetic pump-based nanospray sheath liquid CE/MS coupling technology holds promise for on-line quantitation and identification of N-linked glycans on recombinant therapeutic proteins.
29575162	0	31	theme	Teal™	112:116	arg1	dye					130:132	Teal™ fluorescent dye	112:132	Teal™ fluorescent dye	112:132	On-line capillary electrophoresis/laser-induced fluorescence/mass spectrometry analysis of glycans labeled with Teal™ fluorescent dye using an electrokinetic sheath liquid pump-based nanospray ion source.
29575162	1	32	theme	glycosylation	419:431	arg1	profile					433:439	glycosylation profile	419:439	glycosylation profile	419:439	RATIONALE N-linked glycan analysis of recombinant therapeutic proteins, such as monoclonal antibodies, Fc-fusion proteins, and antibody-drug conjugates, provides valuable information regarding protein therapeutics glycosylation profile.
29575162	1	33	theme	RATIONALE	205:213	arg1	analysis					231:238	RATIONALE N-linked glycan analysis	205:238	RATIONALE N-linked glycan analysis of recombinant therapeutic proteins, such as monoclonal antibodies, Fc-fusion proteins, and antibody-drug conjugates,	205:356	RATIONALE N-linked glycan analysis of recombinant therapeutic proteins, such as monoclonal antibodies, Fc-fusion proteins, and antibody-drug conjugates, provides valuable information regarding protein therapeutics glycosylation profile.
29575162	2	34	theme	biotherapeutic	640:653	arg1	development					623:633	the development	619:633	the development of a biotherapeutic	619:653	Both qualitative identification and quantitative analysis of N-linked glycans on recombinant therapeutic proteins are critical analytical tasks in the biopharma industry during the development of a biotherapeutic.
29575162	8	35	theme	liquid	1767:1772	arg1	technology					1789:1798	electrokinetic pump-based nanospray sheath liquid CE/MS coupling technology	1724:1798	electrokinetic pump-based nanospray sheath liquid CE/MS coupling technology	1724:1798	CONCLUSIONS This on-line CE/LIF/MS method using Teal™ fluorescent dye and electrokinetic pump-based nanospray sheath liquid CE/MS coupling technology holds promise for on-line quantitation and identification of N-linked glycans on recombinant therapeutic proteins.
29575162	5	36	theme	analysis	1131:1138	arg1	workflow					1140:1147	an on-line CE/LIF/MS N-glycan analysis workflow	1101:1147	an on-line CE/LIF/MS N-glycan analysis workflow that incorporates the fluorescent Teal™ dye and an electrokinetic pump-based nanospray sheath liquid capillary electrophoresis/mass spectrometry (CE/MS) ion source	1101:1311	Here we present an on-line CE/LIF/MS N-glycan analysis workflow that incorporates the fluorescent Teal™ dye and an electrokinetic pump-based nanospray sheath liquid capillary electrophoresis/mass spectrometry (CE/MS) ion source.
29575162	8	37	theme	coupling	1780:1787	arg1	technology					1789:1798	electrokinetic pump-based nanospray sheath liquid CE/MS coupling technology	1724:1798	electrokinetic pump-based nanospray sheath liquid CE/MS coupling technology	1724:1798	CONCLUSIONS This on-line CE/LIF/MS method using Teal™ fluorescent dye and electrokinetic pump-based nanospray sheath liquid CE/MS coupling technology holds promise for on-line quantitation and identification of N-linked glycans on recombinant therapeutic proteins.
29575162	7	38	theme	common	1615:1620	arg1	platforms					1639:1647	common CE/MS instrument platforms	1615:1647	common CE/MS instrument platforms	1615:1647	Results show that on-line CE/LIF/MS analysis can be readily achieved using this versatile CE/MS ion source on common CE/MS instrument platforms.
29575162	5	39	theme	ion	1302:1304	arg1	source					1306:1311	ion source	1302:1311	ion source	1302:1311	Here we present an on-line CE/LIF/MS N-glycan analysis workflow that incorporates the fluorescent Teal™ dye and an electrokinetic pump-based nanospray sheath liquid capillary electrophoresis/mass spectrometry (CE/MS) ion source.
29575162	2	40	theme	critical	560:567	arg1	analysis					491:498	quantitative analysis	478:498	quantitative analysis of N-linked glycans on recombinant therapeutic proteins	478:554	Both qualitative identification and quantitative analysis of N-linked glycans on recombinant therapeutic proteins are critical analytical tasks in the biopharma industry during the development of a biotherapeutic.
29575162	2	40	theme	critical	560:567	arg1	tasks					580:584	critical analytical tasks	560:584	critical analytical tasks	560:584	Both qualitative identification and quantitative analysis of N-linked glycans on recombinant therapeutic proteins are critical analytical tasks in the biopharma industry during the development of a biotherapeutic.
29575162	2	40	theme	critical	560:567	arg1	identification					459:472	qualitative identification	447:472	qualitative identification	447:472	Both qualitative identification and quantitative analysis of N-linked glycans on recombinant therapeutic proteins are critical analytical tasks in the biopharma industry during the development of a biotherapeutic.
29575162	1	41	link	N-linked	215:222	arg1	analysis					231:238	RATIONALE N-linked glycan analysis	205:238	RATIONALE N-linked glycan analysis of recombinant therapeutic proteins, such as monoclonal antibodies, Fc-fusion proteins, and antibody-drug conjugates,	205:356	RATIONALE N-linked glycan analysis of recombinant therapeutic proteins, such as monoclonal antibodies, Fc-fusion proteins, and antibody-drug conjugates, provides valuable information regarding protein therapeutics glycosylation profile.
29575162	4	42	theme	N-linked	909:916	arg1	glycans					918:924	N-linked glycans	909:924	N-linked glycans	909:924	N-linked glycans are first released from glycoproteins by enzymatic digestion, then labeled with fluorescence dyes for subsequent CE or LC separation, and LIF or MS detection.
29575162	2	43	theme	quantitative	478:489	arg1	analysis					491:498	quantitative analysis	478:498	quantitative analysis of N-linked glycans on recombinant therapeutic proteins	478:554	Both qualitative identification and quantitative analysis of N-linked glycans on recombinant therapeutic proteins are critical analytical tasks in the biopharma industry during the development of a biotherapeutic.
29575162	2	43	theme	quantitative	478:489	arg1	tasks					580:584	critical analytical tasks	560:584	critical analytical tasks	560:584	Both qualitative identification and quantitative analysis of N-linked glycans on recombinant therapeutic proteins are critical analytical tasks in the biopharma industry during the development of a biotherapeutic.
29575162	2	43	theme	quantitative	478:489	arg1	identification					459:472	qualitative identification	447:472	qualitative identification	447:472	Both qualitative identification and quantitative analysis of N-linked glycans on recombinant therapeutic proteins are critical analytical tasks in the biopharma industry during the development of a biotherapeutic.
29575162	0	44	theme	On-line	0:6	arg1	spectrometry					66:77	On-line capillary electrophoresis/laser-induced fluorescence/mass spectrometry	0:77	On-line capillary electrophoresis/laser-induced fluorescence/mass spectrometry analysis of glycans	0:97	On-line capillary electrophoresis/laser-induced fluorescence/mass spectrometry analysis of glycans labeled with Teal™ fluorescent dye using an electrokinetic sheath liquid pump-based nanospray ion source.
29575162	2	45	theme	recombinant	523:533	arg1	proteins					547:554	recombinant therapeutic proteins	523:554	recombinant therapeutic proteins	523:554	Both qualitative identification and quantitative analysis of N-linked glycans on recombinant therapeutic proteins are critical analytical tasks in the biopharma industry during the development of a biotherapeutic.
29575162	0	46	theme	electrophoresis/laser-induced	18:46	arg1	spectrometry					66:77	On-line capillary electrophoresis/laser-induced fluorescence/mass spectrometry	0:77	On-line capillary electrophoresis/laser-induced fluorescence/mass spectrometry analysis of glycans	0:97	On-line capillary electrophoresis/laser-induced fluorescence/mass spectrometry analysis of glycans labeled with Teal™ fluorescent dye using an electrokinetic sheath liquid pump-based nanospray ion source.
29575162	5	47	theme	CE/LIF/MS	1112:1120	arg1	workflow					1140:1147	an on-line CE/LIF/MS N-glycan analysis workflow	1101:1147	an on-line CE/LIF/MS N-glycan analysis workflow that incorporates the fluorescent Teal™ dye and an electrokinetic pump-based nanospray sheath liquid capillary electrophoresis/mass spectrometry (CE/MS) ion source	1101:1311	Here we present an on-line CE/LIF/MS N-glycan analysis workflow that incorporates the fluorescent Teal™ dye and an electrokinetic pump-based nanospray sheath liquid capillary electrophoresis/mass spectrometry (CE/MS) ion source.
29575162	1	48	dep	therapeutics	406:417	arg1	profile					433:439	glycosylation profile	419:439	glycosylation profile	419:439	RATIONALE N-linked glycan analysis of recombinant therapeutic proteins, such as monoclonal antibodies, Fc-fusion proteins, and antibody-drug conjugates, provides valuable information regarding protein therapeutics glycosylation profile.
29575162	3	49	theme	chromatography/fluorescence	788:814	arg1	technologies					895:906	capillary electrophoresis/laser-induced fluorescence (CE/LIF), liquid chromatography/fluorescence (LC/FLR), and liquid chromatography/fluorescence/mass spectrometry (LC/FLR/MS) technologies	718:906	capillary electrophoresis/laser-induced fluorescence (CE/LIF), liquid chromatography/fluorescence (LC/FLR), and liquid chromatography/fluorescence/mass spectrometry (LC/FLR/MS) technologies	718:906	METHODS Currently, such analyses are mainly carried out using capillary electrophoresis/laser-induced fluorescence (CE/LIF), liquid chromatography/fluorescence (LC/FLR), and liquid chromatography/fluorescence/mass spectrometry (LC/FLR/MS) technologies.
29575162	2	50	theme	glycans	512:518	arg1	analysis					491:498	quantitative analysis	478:498	quantitative analysis of N-linked glycans on recombinant therapeutic proteins	478:554	Both qualitative identification and quantitative analysis of N-linked glycans on recombinant therapeutic proteins are critical analytical tasks in the biopharma industry during the development of a biotherapeutic.
29575162	2	50	theme	glycans	512:518	arg1	tasks					580:584	critical analytical tasks	560:584	critical analytical tasks	560:584	Both qualitative identification and quantitative analysis of N-linked glycans on recombinant therapeutic proteins are critical analytical tasks in the biopharma industry during the development of a biotherapeutic.
29575162	2	50	theme	glycans	512:518	arg1	identification					459:472	qualitative identification	447:472	qualitative identification	447:472	Both qualitative identification and quantitative analysis of N-linked glycans on recombinant therapeutic proteins are critical analytical tasks in the biopharma industry during the development of a biotherapeutic.
29575162	0	51	theme	spectrometry	66:77	arg1	analysis					79:86	On-line capillary electrophoresis/laser-induced fluorescence/mass spectrometry analysis	0:86	On-line capillary electrophoresis/laser-induced fluorescence/mass spectrometry analysis of glycans	0:97	On-line capillary electrophoresis/laser-induced fluorescence/mass spectrometry analysis of glycans labeled with Teal™ fluorescent dye using an electrokinetic sheath liquid pump-based nanospray ion source.
29575162	7	52	theme	on-line	1523:1529	arg1	analysis					1541:1548	on-line CE/LIF/MS analysis	1523:1548	on-line CE/LIF/MS analysis	1523:1548	Results show that on-line CE/LIF/MS analysis can be readily achieved using this versatile CE/MS ion source on common CE/MS instrument platforms.
29575162	4	53	theme	fluorescence	1006:1017	arg1	dyes					1019:1022	fluorescence dyes	1006:1022	fluorescence dyes for subsequent CE or LC separation, and LIF or MS detection	1006:1082	N-linked glycans are first released from glycoproteins by enzymatic digestion, then labeled with fluorescence dyes for subsequent CE or LC separation, and LIF or MS detection.
29575162	8	54	theme	sheath	1760:1765	arg1	technology					1789:1798	electrokinetic pump-based nanospray sheath liquid CE/MS coupling technology	1724:1798	electrokinetic pump-based nanospray sheath liquid CE/MS coupling technology	1724:1798	CONCLUSIONS This on-line CE/LIF/MS method using Teal™ fluorescent dye and electrokinetic pump-based nanospray sheath liquid CE/MS coupling technology holds promise for on-line quantitation and identification of N-linked glycans on recombinant therapeutic proteins.
29575162	5	55	theme	electrokinetic	1200:1213	arg1	CE/MS					1295:1299	CE/MS	1295:1299	CE/MS	1295:1299	Here we present an on-line CE/LIF/MS N-glycan analysis workflow that incorporates the fluorescent Teal™ dye and an electrokinetic pump-based nanospray sheath liquid capillary electrophoresis/mass spectrometry (CE/MS) ion source.
29575162	5	55	theme	electrokinetic	1200:1213	arg1	spectrometry					1281:1292	an electrokinetic pump-based nanospray sheath liquid capillary electrophoresis/mass spectrometry	1197:1292	an electrokinetic pump-based nanospray sheath liquid capillary electrophoresis/mass spectrometry (CE/MS)	1197:1300	Here we present an on-line CE/LIF/MS N-glycan analysis workflow that incorporates the fluorescent Teal™ dye and an electrokinetic pump-based nanospray sheath liquid capillary electrophoresis/mass spectrometry (CE/MS) ion source.
29575162	8	56	theme	N-linked	1861:1868	arg1	glycans					1870:1876	N-linked glycans	1861:1876	N-linked glycans	1861:1876	CONCLUSIONS This on-line CE/LIF/MS method using Teal™ fluorescent dye and electrokinetic pump-based nanospray sheath liquid CE/MS coupling technology holds promise for on-line quantitation and identification of N-linked glycans on recombinant therapeutic proteins.
29575162	2	57	link	N-linked	503:510	arg1	glycans					512:518	N-linked glycans	503:518	N-linked glycans on recombinant therapeutic proteins	503:554	Both qualitative identification and quantitative analysis of N-linked glycans on recombinant therapeutic proteins are critical analytical tasks in the biopharma industry during the development of a biotherapeutic.
29575162	4	58	theme	enzymatic	967:975	arg1	digestion					977:985	enzymatic digestion	967:985	enzymatic digestion	967:985	N-linked glycans are first released from glycoproteins by enzymatic digestion, then labeled with fluorescence dyes for subsequent CE or LC separation, and LIF or MS detection.
29575162	2	59	from	glycans	512:518	arg1	proteins					547:554	recombinant therapeutic proteins	523:554	recombinant therapeutic proteins	523:554	Both qualitative identification and quantitative analysis of N-linked glycans on recombinant therapeutic proteins are critical analytical tasks in the biopharma industry during the development of a biotherapeutic.
29575162	8	60	theme	CE/LIF/MS	1675:1683	arg1	method					1685:1690	This on-line CE/LIF/MS method	1662:1690	This on-line CE/LIF/MS method using Teal™ fluorescent dye and electrokinetic pump-based nanospray sheath liquid CE/MS coupling technology	1662:1798	CONCLUSIONS This on-line CE/LIF/MS method using Teal™ fluorescent dye and electrokinetic pump-based nanospray sheath liquid CE/MS coupling technology holds promise for on-line quantitation and identification of N-linked glycans on recombinant therapeutic proteins.
29575162	1	61	theme	monoclonal	285:294	arg1	antibodies					296:305	monoclonal antibodies	285:305	monoclonal antibodies	285:305	RATIONALE N-linked glycan analysis of recombinant therapeutic proteins, such as monoclonal antibodies, Fc-fusion proteins, and antibody-drug conjugates, provides valuable information regarding protein therapeutics glycosylation profile.
29575162	5	62	theme	Teal™	1183:1187	arg1	dye					1189:1191	the fluorescent Teal™ dye	1167:1191	the fluorescent Teal™ dye	1167:1191	Here we present an on-line CE/LIF/MS N-glycan analysis workflow that incorporates the fluorescent Teal™ dye and an electrokinetic pump-based nanospray sheath liquid capillary electrophoresis/mass spectrometry (CE/MS) ion source.
29575162	8	63	theme	therapeutic	1893:1903	arg1	proteins					1905:1912	recombinant therapeutic proteins	1881:1912	recombinant therapeutic proteins	1881:1912	CONCLUSIONS This on-line CE/LIF/MS method using Teal™ fluorescent dye and electrokinetic pump-based nanospray sheath liquid CE/MS coupling technology holds promise for on-line quantitation and identification of N-linked glycans on recombinant therapeutic proteins.
29575162	3	64	theme	spectrometry	870:881	arg1	technologies					895:906	capillary electrophoresis/laser-induced fluorescence (CE/LIF), liquid chromatography/fluorescence (LC/FLR), and liquid chromatography/fluorescence/mass spectrometry (LC/FLR/MS) technologies	718:906	capillary electrophoresis/laser-induced fluorescence (CE/LIF), liquid chromatography/fluorescence (LC/FLR), and liquid chromatography/fluorescence/mass spectrometry (LC/FLR/MS) technologies	718:906	METHODS Currently, such analyses are mainly carried out using capillary electrophoresis/laser-induced fluorescence (CE/LIF), liquid chromatography/fluorescence (LC/FLR), and liquid chromatography/fluorescence/mass spectrometry (LC/FLR/MS) technologies.
29575162	3	65	theme	liquid	830:835	arg1	LC/FLR/MS					884:892	LC/FLR/MS	884:892	LC/FLR/MS	884:892	METHODS Currently, such analyses are mainly carried out using capillary electrophoresis/laser-induced fluorescence (CE/LIF), liquid chromatography/fluorescence (LC/FLR), and liquid chromatography/fluorescence/mass spectrometry (LC/FLR/MS) technologies.
29575162	3	65	theme	liquid	830:835	arg1	spectrometry					870:881	liquid chromatography/fluorescence/mass spectrometry	830:881	liquid chromatography/fluorescence/mass spectrometry (LC/FLR/MS)	830:893	METHODS Currently, such analyses are mainly carried out using capillary electrophoresis/laser-induced fluorescence (CE/LIF), liquid chromatography/fluorescence (LC/FLR), and liquid chromatography/fluorescence/mass spectrometry (LC/FLR/MS) technologies.
29575162	6	66	theme	ammonium	1388:1395	arg1	hydroxide					1397:1405	ammonium hydroxide	1388:1405	ammonium hydroxide	1388:1405	RESULTS Electrophoresis running buffer systems using ammonium acetate and ammonium hydroxide were developed for the negative ion mode CE/MS analysis of fluorescence-labeled N-linked glycans.
29575162	6	67	theme	N-linked	1487:1494	arg1	glycans					1496:1502	fluorescence-labeled N-linked glycans	1466:1502	fluorescence-labeled N-linked glycans	1466:1502	RESULTS Electrophoresis running buffer systems using ammonium acetate and ammonium hydroxide were developed for the negative ion mode CE/MS analysis of fluorescence-labeled N-linked glycans.
29575162	1	68	theme	N-linked	215:222	arg1	analysis					231:238	RATIONALE N-linked glycan analysis	205:238	RATIONALE N-linked glycan analysis of recombinant therapeutic proteins, such as monoclonal antibodies, Fc-fusion proteins, and antibody-drug conjugates,	205:356	RATIONALE N-linked glycan analysis of recombinant therapeutic proteins, such as monoclonal antibodies, Fc-fusion proteins, and antibody-drug conjugates, provides valuable information regarding protein therapeutics glycosylation profile.
29575162	4	69	gly	glycoproteins	950:962	arg1	glycoproteins					950:962	glycoproteins	950:962	glycoproteins	950:962	N-linked glycans are first released from glycoproteins by enzymatic digestion, then labeled with fluorescence dyes for subsequent CE or LC separation, and LIF or MS detection.
29575162	4	70	theme	subsequent	1028:1037	arg1	CE					1039:1040	subsequent CE	1028:1040	subsequent CE	1028:1040	N-linked glycans are first released from glycoproteins by enzymatic digestion, then labeled with fluorescence dyes for subsequent CE or LC separation, and LIF or MS detection.
29575162	2	71	from	identification	459:472	arg1	proteins					547:554	recombinant therapeutic proteins	523:554	recombinant therapeutic proteins	523:554	Both qualitative identification and quantitative analysis of N-linked glycans on recombinant therapeutic proteins are critical analytical tasks in the biopharma industry during the development of a biotherapeutic.
29575162	8	72	link	N-linked	1861:1868	arg1	glycans					1870:1876	N-linked glycans	1861:1876	N-linked glycans	1861:1876	CONCLUSIONS This on-line CE/LIF/MS method using Teal™ fluorescent dye and electrokinetic pump-based nanospray sheath liquid CE/MS coupling technology holds promise for on-line quantitation and identification of N-linked glycans on recombinant therapeutic proteins.
29575162	4	73	attach	released	936:943	arg1	glycoproteins					950:962	glycoproteins	950:962	glycoproteins	950:962	N-linked glycans are first released from glycoproteins by enzymatic digestion, then labeled with fluorescence dyes for subsequent CE or LC separation, and LIF or MS detection.
29575162	4	73	attach	released	936:943	arg2	glycans					918:924	N-linked glycans	909:924	N-linked glycans	909:924	N-linked glycans are first released from glycoproteins by enzymatic digestion, then labeled with fluorescence dyes for subsequent CE or LC separation, and LIF or MS detection.
29575162	0	74	theme	liquid	165:170	arg1	source					197:202	an electrokinetic sheath liquid pump-based nanospray ion source	140:202	an electrokinetic sheath liquid pump-based nanospray ion source	140:202	On-line capillary electrophoresis/laser-induced fluorescence/mass spectrometry analysis of glycans labeled with Teal™ fluorescent dye using an electrokinetic sheath liquid pump-based nanospray ion source.
29575162	3	75	theme	such	675:678	arg1	analyses					680:687	such analyses	675:687	such analyses	675:687	METHODS Currently, such analyses are mainly carried out using capillary electrophoresis/laser-induced fluorescence (CE/LIF), liquid chromatography/fluorescence (LC/FLR), and liquid chromatography/fluorescence/mass spectrometry (LC/FLR/MS) technologies.
29575162	1	76	theme	recombinant	243:253	arg1	conjugates					346:355	antibody-drug conjugates	332:355	antibody-drug conjugates	332:355	RATIONALE N-linked glycan analysis of recombinant therapeutic proteins, such as monoclonal antibodies, Fc-fusion proteins, and antibody-drug conjugates, provides valuable information regarding protein therapeutics glycosylation profile.
29575162	1	76	theme	recombinant	243:253	arg1	proteins					318:325	Fc-fusion proteins	308:325	Fc-fusion proteins	308:325	RATIONALE N-linked glycan analysis of recombinant therapeutic proteins, such as monoclonal antibodies, Fc-fusion proteins, and antibody-drug conjugates, provides valuable information regarding protein therapeutics glycosylation profile.
29575162	1	76	theme	recombinant	243:253	arg1	antibodies					296:305	monoclonal antibodies	285:305	monoclonal antibodies	285:305	RATIONALE N-linked glycan analysis of recombinant therapeutic proteins, such as monoclonal antibodies, Fc-fusion proteins, and antibody-drug conjugates, provides valuable information regarding protein therapeutics glycosylation profile.
29575162	1	76	theme	recombinant	243:253	arg1	proteins					267:274	recombinant therapeutic proteins	243:274	recombinant therapeutic proteins	243:274	RATIONALE N-linked glycan analysis of recombinant therapeutic proteins, such as monoclonal antibodies, Fc-fusion proteins, and antibody-drug conjugates, provides valuable information regarding protein therapeutics glycosylation profile.
29575162	6	77	theme	buffer	1346:1351	arg1	systems					1353:1359	running buffer systems	1338:1359	running buffer systems using ammonium acetate and ammonium hydroxide	1338:1405	RESULTS Electrophoresis running buffer systems using ammonium acetate and ammonium hydroxide were developed for the negative ion mode CE/MS analysis of fluorescence-labeled N-linked glycans.
29575162	6	78	link	N-linked	1487:1494	arg1	glycans					1496:1502	fluorescence-labeled N-linked glycans	1466:1502	fluorescence-labeled N-linked glycans	1466:1502	RESULTS Electrophoresis running buffer systems using ammonium acetate and ammonium hydroxide were developed for the negative ion mode CE/MS analysis of fluorescence-labeled N-linked glycans.
29575162	0	79	theme	nanospray	183:191	arg1	source					197:202	an electrokinetic sheath liquid pump-based nanospray ion source	140:202	an electrokinetic sheath liquid pump-based nanospray ion source	140:202	On-line capillary electrophoresis/laser-induced fluorescence/mass spectrometry analysis of glycans labeled with Teal™ fluorescent dye using an electrokinetic sheath liquid pump-based nanospray ion source.
29575162	5	80	theme	electrophoresis/mass	1260:1279	arg1	CE/MS					1295:1299	CE/MS	1295:1299	CE/MS	1295:1299	Here we present an on-line CE/LIF/MS N-glycan analysis workflow that incorporates the fluorescent Teal™ dye and an electrokinetic pump-based nanospray sheath liquid capillary electrophoresis/mass spectrometry (CE/MS) ion source.
29575162	5	80	theme	electrophoresis/mass	1260:1279	arg1	spectrometry					1281:1292	an electrokinetic pump-based nanospray sheath liquid capillary electrophoresis/mass spectrometry	1197:1292	an electrokinetic pump-based nanospray sheath liquid capillary electrophoresis/mass spectrometry (CE/MS)	1197:1300	Here we present an on-line CE/LIF/MS N-glycan analysis workflow that incorporates the fluorescent Teal™ dye and an electrokinetic pump-based nanospray sheath liquid capillary electrophoresis/mass spectrometry (CE/MS) ion source.
29575162	1	81	theme	proteins	267:274	arg1	analysis					231:238	RATIONALE N-linked glycan analysis	205:238	RATIONALE N-linked glycan analysis of recombinant therapeutic proteins, such as monoclonal antibodies, Fc-fusion proteins, and antibody-drug conjugates,	205:356	RATIONALE N-linked glycan analysis of recombinant therapeutic proteins, such as monoclonal antibodies, Fc-fusion proteins, and antibody-drug conjugates, provides valuable information regarding protein therapeutics glycosylation profile.
29575162	8	82	theme	fluorescent	1704:1714	arg1	dye					1716:1718	Teal™ fluorescent dye	1698:1718	Teal™ fluorescent dye	1698:1718	CONCLUSIONS This on-line CE/LIF/MS method using Teal™ fluorescent dye and electrokinetic pump-based nanospray sheath liquid CE/MS coupling technology holds promise for on-line quantitation and identification of N-linked glycans on recombinant therapeutic proteins.
29575162	6	83	theme	mode	1443:1446	arg1	analysis					1454:1461	the negative ion mode CE/MS analysis	1426:1461	the negative ion mode CE/MS analysis of fluorescence-labeled N-linked glycans	1426:1502	RESULTS Electrophoresis running buffer systems using ammonium acetate and ammonium hydroxide were developed for the negative ion mode CE/MS analysis of fluorescence-labeled N-linked glycans.
29575162	6	84	theme	negative	1430:1437	arg1	mode					1443:1446	the negative ion mode	1426:1446	the negative ion mode CE/MS analysis of fluorescence-labeled N-linked glycans	1426:1502	RESULTS Electrophoresis running buffer systems using ammonium acetate and ammonium hydroxide were developed for the negative ion mode CE/MS analysis of fluorescence-labeled N-linked glycans.
29575162	5	85	theme	nanospray	1226:1234	arg1	CE/MS					1295:1299	CE/MS	1295:1299	CE/MS	1295:1299	Here we present an on-line CE/LIF/MS N-glycan analysis workflow that incorporates the fluorescent Teal™ dye and an electrokinetic pump-based nanospray sheath liquid capillary electrophoresis/mass spectrometry (CE/MS) ion source.
29575162	5	85	theme	nanospray	1226:1234	arg1	spectrometry					1281:1292	an electrokinetic pump-based nanospray sheath liquid capillary electrophoresis/mass spectrometry	1197:1292	an electrokinetic pump-based nanospray sheath liquid capillary electrophoresis/mass spectrometry (CE/MS)	1197:1300	Here we present an on-line CE/LIF/MS N-glycan analysis workflow that incorporates the fluorescent Teal™ dye and an electrokinetic pump-based nanospray sheath liquid capillary electrophoresis/mass spectrometry (CE/MS) ion source.
29575162	8	86	theme	pump-based	1739:1748	arg1	technology					1789:1798	electrokinetic pump-based nanospray sheath liquid CE/MS coupling technology	1724:1798	electrokinetic pump-based nanospray sheath liquid CE/MS coupling technology	1724:1798	CONCLUSIONS This on-line CE/LIF/MS method using Teal™ fluorescent dye and electrokinetic pump-based nanospray sheath liquid CE/MS coupling technology holds promise for on-line quantitation and identification of N-linked glycans on recombinant therapeutic proteins.
29575162	7	87	theme	CE/MS	1595:1599	arg1	source					1605:1610	this versatile CE/MS ion source	1580:1610	this versatile CE/MS ion source	1580:1610	Results show that on-line CE/LIF/MS analysis can be readily achieved using this versatile CE/MS ion source on common CE/MS instrument platforms.
29575162	5	88	theme	liquid	1243:1248	arg1	CE/MS					1295:1299	CE/MS	1295:1299	CE/MS	1295:1299	Here we present an on-line CE/LIF/MS N-glycan analysis workflow that incorporates the fluorescent Teal™ dye and an electrokinetic pump-based nanospray sheath liquid capillary electrophoresis/mass spectrometry (CE/MS) ion source.
29575162	5	88	theme	liquid	1243:1248	arg1	spectrometry					1281:1292	an electrokinetic pump-based nanospray sheath liquid capillary electrophoresis/mass spectrometry	1197:1292	an electrokinetic pump-based nanospray sheath liquid capillary electrophoresis/mass spectrometry (CE/MS)	1197:1300	Here we present an on-line CE/LIF/MS N-glycan analysis workflow that incorporates the fluorescent Teal™ dye and an electrokinetic pump-based nanospray sheath liquid capillary electrophoresis/mass spectrometry (CE/MS) ion source.
29575162	3	89	theme	electrophoresis/laser-induced	728:756	arg1	fluorescence					758:769	electrophoresis/laser-induced fluorescence	728:769	electrophoresis/laser-induced fluorescence (CE/LIF)	728:778	METHODS Currently, such analyses are mainly carried out using capillary electrophoresis/laser-induced fluorescence (CE/LIF), liquid chromatography/fluorescence (LC/FLR), and liquid chromatography/fluorescence/mass spectrometry (LC/FLR/MS) technologies.
29575162	3	89	theme	electrophoresis/laser-induced	728:756	arg1	CE/LIF					772:777	CE/LIF	772:777	CE/LIF	772:777	METHODS Currently, such analyses are mainly carried out using capillary electrophoresis/laser-induced fluorescence (CE/LIF), liquid chromatography/fluorescence (LC/FLR), and liquid chromatography/fluorescence/mass spectrometry (LC/FLR/MS) technologies.
29575162	2	90	from	proteins	547:554	arg1	analysis					491:498	quantitative analysis	478:498	quantitative analysis of N-linked glycans on recombinant therapeutic proteins	478:554	Both qualitative identification and quantitative analysis of N-linked glycans on recombinant therapeutic proteins are critical analytical tasks in the biopharma industry during the development of a biotherapeutic.
29575162	2	90	from	proteins	547:554	arg1	tasks					580:584	critical analytical tasks	560:584	critical analytical tasks	560:584	Both qualitative identification and quantitative analysis of N-linked glycans on recombinant therapeutic proteins are critical analytical tasks in the biopharma industry during the development of a biotherapeutic.
29575162	2	90	from	proteins	547:554	arg1	identification					459:472	qualitative identification	447:472	qualitative identification	447:472	Both qualitative identification and quantitative analysis of N-linked glycans on recombinant therapeutic proteins are critical analytical tasks in the biopharma industry during the development of a biotherapeutic.
29575162	0	91	theme	fluorescent	118:128	arg1	dye					130:132	Teal™ fluorescent dye	112:132	Teal™ fluorescent dye	112:132	On-line capillary electrophoresis/laser-induced fluorescence/mass spectrometry analysis of glycans labeled with Teal™ fluorescent dye using an electrokinetic sheath liquid pump-based nanospray ion source.
29575162	2	92	theme	biopharma	593:601	arg1	industry					603:610	the biopharma industry	589:610	the biopharma industry	589:610	Both qualitative identification and quantitative analysis of N-linked glycans on recombinant therapeutic proteins are critical analytical tasks in the biopharma industry during the development of a biotherapeutic.
29575162	8	93	theme	CE/MS	1774:1778	arg1	technology					1789:1798	electrokinetic pump-based nanospray sheath liquid CE/MS coupling technology	1724:1798	electrokinetic pump-based nanospray sheath liquid CE/MS coupling technology	1724:1798	CONCLUSIONS This on-line CE/LIF/MS method using Teal™ fluorescent dye and electrokinetic pump-based nanospray sheath liquid CE/MS coupling technology holds promise for on-line quantitation and identification of N-linked glycans on recombinant therapeutic proteins.
29575162	5	94	theme	N-glycan	1122:1129	arg1	workflow					1140:1147	an on-line CE/LIF/MS N-glycan analysis workflow	1101:1147	an on-line CE/LIF/MS N-glycan analysis workflow that incorporates the fluorescent Teal™ dye and an electrokinetic pump-based nanospray sheath liquid capillary electrophoresis/mass spectrometry (CE/MS) ion source	1101:1311	Here we present an on-line CE/LIF/MS N-glycan analysis workflow that incorporates the fluorescent Teal™ dye and an electrokinetic pump-based nanospray sheath liquid capillary electrophoresis/mass spectrometry (CE/MS) ion source.
29575162	7	95	theme	instrument	1628:1637	arg1	platforms					1639:1647	common CE/MS instrument platforms	1615:1647	common CE/MS instrument platforms	1615:1647	Results show that on-line CE/LIF/MS analysis can be readily achieved using this versatile CE/MS ion source on common CE/MS instrument platforms.
29575162	2	96	from	tasks	580:584	arg1	industry					603:610	the biopharma industry	589:610	the biopharma industry	589:610	Both qualitative identification and quantitative analysis of N-linked glycans on recombinant therapeutic proteins are critical analytical tasks in the biopharma industry during the development of a biotherapeutic.
29575162	0	97	theme	electrokinetic	143:156	arg1	source					197:202	an electrokinetic sheath liquid pump-based nanospray ion source	140:202	an electrokinetic sheath liquid pump-based nanospray ion source	140:202	On-line capillary electrophoresis/laser-induced fluorescence/mass spectrometry analysis of glycans labeled with Teal™ fluorescent dye using an electrokinetic sheath liquid pump-based nanospray ion source.
29575162	0	98	theme	capillary	8:16	arg1	spectrometry					66:77	On-line capillary electrophoresis/laser-induced fluorescence/mass spectrometry	0:77	On-line capillary electrophoresis/laser-induced fluorescence/mass spectrometry analysis of glycans	0:97	On-line capillary electrophoresis/laser-induced fluorescence/mass spectrometry analysis of glycans labeled with Teal™ fluorescent dye using an electrokinetic sheath liquid pump-based nanospray ion source.
29575162	8	99	theme	on-line	1818:1824	arg1	quantitation					1826:1837	on-line quantitation	1818:1837	on-line quantitation	1818:1837	CONCLUSIONS This on-line CE/LIF/MS method using Teal™ fluorescent dye and electrokinetic pump-based nanospray sheath liquid CE/MS coupling technology holds promise for on-line quantitation and identification of N-linked glycans on recombinant therapeutic proteins.
29575162	0	100	theme	fluorescence/mass	48:64	arg1	spectrometry					66:77	On-line capillary electrophoresis/laser-induced fluorescence/mass spectrometry	0:77	On-line capillary electrophoresis/laser-induced fluorescence/mass spectrometry analysis of glycans	0:97	On-line capillary electrophoresis/laser-induced fluorescence/mass spectrometry analysis of glycans labeled with Teal™ fluorescent dye using an electrokinetic sheath liquid pump-based nanospray ion source.
29575162	1	101	theme	antibody-drug	332:344	arg1	conjugates					346:355	antibody-drug conjugates	332:355	antibody-drug conjugates	332:355	RATIONALE N-linked glycan analysis of recombinant therapeutic proteins, such as monoclonal antibodies, Fc-fusion proteins, and antibody-drug conjugates, provides valuable information regarding protein therapeutics glycosylation profile.
29575162	5	102	theme	on-line	1104:1110	arg1	workflow					1140:1147	an on-line CE/LIF/MS N-glycan analysis workflow	1101:1147	an on-line CE/LIF/MS N-glycan analysis workflow that incorporates the fluorescent Teal™ dye and an electrokinetic pump-based nanospray sheath liquid capillary electrophoresis/mass spectrometry (CE/MS) ion source	1101:1311	Here we present an on-line CE/LIF/MS N-glycan analysis workflow that incorporates the fluorescent Teal™ dye and an electrokinetic pump-based nanospray sheath liquid capillary electrophoresis/mass spectrometry (CE/MS) ion source.
29575162	7	103	theme	CE/LIF/MS	1531:1539	arg1	analysis					1541:1548	on-line CE/LIF/MS analysis	1523:1548	on-line CE/LIF/MS analysis	1523:1548	Results show that on-line CE/LIF/MS analysis can be readily achieved using this versatile CE/MS ion source on common CE/MS instrument platforms.
29575162	2	104	theme	therapeutic	535:545	arg1	proteins					547:554	recombinant therapeutic proteins	523:554	recombinant therapeutic proteins	523:554	Both qualitative identification and quantitative analysis of N-linked glycans on recombinant therapeutic proteins are critical analytical tasks in the biopharma industry during the development of a biotherapeutic.
29575162	6	105	dep	RESULTS	1314:1320	arg1	Electrophoresis					1322:1336	Electrophoresis	1322:1336	Electrophoresis	1322:1336	RESULTS Electrophoresis running buffer systems using ammonium acetate and ammonium hydroxide were developed for the negative ion mode CE/MS analysis of fluorescence-labeled N-linked glycans.
29575162	3	106	theme	liquid	781:786	arg1	LC/FLR					817:822	LC/FLR	817:822	LC/FLR	817:822	METHODS Currently, such analyses are mainly carried out using capillary electrophoresis/laser-induced fluorescence (CE/LIF), liquid chromatography/fluorescence (LC/FLR), and liquid chromatography/fluorescence/mass spectrometry (LC/FLR/MS) technologies.
29575162	3	106	theme	liquid	781:786	arg1	chromatography/fluorescence					788:814	liquid chromatography/fluorescence	781:814	liquid chromatography/fluorescence (LC/FLR)	781:823	METHODS Currently, such analyses are mainly carried out using capillary electrophoresis/laser-induced fluorescence (CE/LIF), liquid chromatography/fluorescence (LC/FLR), and liquid chromatography/fluorescence/mass spectrometry (LC/FLR/MS) technologies.
29575162	2	107	theme	N-linked	503:510	arg1	glycans					512:518	N-linked glycans	503:518	N-linked glycans on recombinant therapeutic proteins	503:554	Both qualitative identification and quantitative analysis of N-linked glycans on recombinant therapeutic proteins are critical analytical tasks in the biopharma industry during the development of a biotherapeutic.
29575162	0	108	theme	glycans	91:97	arg1	analysis					79:86	On-line capillary electrophoresis/laser-induced fluorescence/mass spectrometry analysis	0:86	On-line capillary electrophoresis/laser-induced fluorescence/mass spectrometry analysis of glycans	0:97	On-line capillary electrophoresis/laser-induced fluorescence/mass spectrometry analysis of glycans labeled with Teal™ fluorescent dye using an electrokinetic sheath liquid pump-based nanospray ion source.
29575162	1	109	theme	valuable	367:374	arg1	information					376:386	valuable information	367:386	valuable information regarding protein therapeutics glycosylation profile	367:439	RATIONALE N-linked glycan analysis of recombinant therapeutic proteins, such as monoclonal antibodies, Fc-fusion proteins, and antibody-drug conjugates, provides valuable information regarding protein therapeutics glycosylation profile.
29575162	6	110	theme	running	1338:1344	arg1	systems					1353:1359	running buffer systems	1338:1359	running buffer systems using ammonium acetate and ammonium hydroxide	1338:1405	RESULTS Electrophoresis running buffer systems using ammonium acetate and ammonium hydroxide were developed for the negative ion mode CE/MS analysis of fluorescence-labeled N-linked glycans.
29575162	2	111	theme	analytical	569:578	arg1	analysis					491:498	quantitative analysis	478:498	quantitative analysis of N-linked glycans on recombinant therapeutic proteins	478:554	Both qualitative identification and quantitative analysis of N-linked glycans on recombinant therapeutic proteins are critical analytical tasks in the biopharma industry during the development of a biotherapeutic.
29575162	2	111	theme	analytical	569:578	arg1	tasks					580:584	critical analytical tasks	560:584	critical analytical tasks	560:584	Both qualitative identification and quantitative analysis of N-linked glycans on recombinant therapeutic proteins are critical analytical tasks in the biopharma industry during the development of a biotherapeutic.
29575162	2	111	theme	analytical	569:578	arg1	identification					459:472	qualitative identification	447:472	qualitative identification	447:472	Both qualitative identification and quantitative analysis of N-linked glycans on recombinant therapeutic proteins are critical analytical tasks in the biopharma industry during the development of a biotherapeutic.
29575162	8	112	theme	glycans	1870:1876	arg1	quantitation					1826:1837	on-line quantitation	1818:1837	on-line quantitation	1818:1837	CONCLUSIONS This on-line CE/LIF/MS method using Teal™ fluorescent dye and electrokinetic pump-based nanospray sheath liquid CE/MS coupling technology holds promise for on-line quantitation and identification of N-linked glycans on recombinant therapeutic proteins.
29575162	8	112	theme	glycans	1870:1876	arg1	identification					1843:1856	identification	1843:1856	identification	1843:1856	CONCLUSIONS This on-line CE/LIF/MS method using Teal™ fluorescent dye and electrokinetic pump-based nanospray sheath liquid CE/MS coupling technology holds promise for on-line quantitation and identification of N-linked glycans on recombinant therapeutic proteins.
29575162	2	113	from	analysis	491:498	arg1	proteins					547:554	recombinant therapeutic proteins	523:554	recombinant therapeutic proteins	523:554	Both qualitative identification and quantitative analysis of N-linked glycans on recombinant therapeutic proteins are critical analytical tasks in the biopharma industry during the development of a biotherapeutic.
29575162	8	114	theme	recombinant	1881:1891	arg1	proteins					1905:1912	recombinant therapeutic proteins	1881:1912	recombinant therapeutic proteins	1881:1912	CONCLUSIONS This on-line CE/LIF/MS method using Teal™ fluorescent dye and electrokinetic pump-based nanospray sheath liquid CE/MS coupling technology holds promise for on-line quantitation and identification of N-linked glycans on recombinant therapeutic proteins.
29575162	5	115	theme	fluorescent	1171:1181	arg1	dye					1189:1191	the fluorescent Teal™ dye	1167:1191	the fluorescent Teal™ dye	1167:1191	Here we present an on-line CE/LIF/MS N-glycan analysis workflow that incorporates the fluorescent Teal™ dye and an electrokinetic pump-based nanospray sheath liquid capillary electrophoresis/mass spectrometry (CE/MS) ion source.
29575162	3	116	theme	chromatography/fluorescence/mass	837:868	arg1	LC/FLR/MS					884:892	LC/FLR/MS	884:892	LC/FLR/MS	884:892	METHODS Currently, such analyses are mainly carried out using capillary electrophoresis/laser-induced fluorescence (CE/LIF), liquid chromatography/fluorescence (LC/FLR), and liquid chromatography/fluorescence/mass spectrometry (LC/FLR/MS) technologies.
29575162	3	116	theme	chromatography/fluorescence/mass	837:868	arg1	spectrometry					870:881	liquid chromatography/fluorescence/mass spectrometry	830:881	liquid chromatography/fluorescence/mass spectrometry (LC/FLR/MS)	830:893	METHODS Currently, such analyses are mainly carried out using capillary electrophoresis/laser-induced fluorescence (CE/LIF), liquid chromatography/fluorescence (LC/FLR), and liquid chromatography/fluorescence/mass spectrometry (LC/FLR/MS) technologies.
29575162	8	117	theme	on-line	1667:1673	arg1	method					1685:1690	This on-line CE/LIF/MS method	1662:1690	This on-line CE/LIF/MS method using Teal™ fluorescent dye and electrokinetic pump-based nanospray sheath liquid CE/MS coupling technology	1662:1798	CONCLUSIONS This on-line CE/LIF/MS method using Teal™ fluorescent dye and electrokinetic pump-based nanospray sheath liquid CE/MS coupling technology holds promise for on-line quantitation and identification of N-linked glycans on recombinant therapeutic proteins.
29575162	2	118	theme	qualitative	447:457	arg1	analysis					491:498	quantitative analysis	478:498	quantitative analysis of N-linked glycans on recombinant therapeutic proteins	478:554	Both qualitative identification and quantitative analysis of N-linked glycans on recombinant therapeutic proteins are critical analytical tasks in the biopharma industry during the development of a biotherapeutic.
29575162	2	118	theme	qualitative	447:457	arg1	tasks					580:584	critical analytical tasks	560:584	critical analytical tasks	560:584	Both qualitative identification and quantitative analysis of N-linked glycans on recombinant therapeutic proteins are critical analytical tasks in the biopharma industry during the development of a biotherapeutic.
29575162	2	118	theme	qualitative	447:457	arg1	identification					459:472	qualitative identification	447:472	qualitative identification	447:472	Both qualitative identification and quantitative analysis of N-linked glycans on recombinant therapeutic proteins are critical analytical tasks in the biopharma industry during the development of a biotherapeutic.
30145464	7	0	theme	exposed	1274:1280	arg1	side					1290:1293	the exposed surface side	1270:1293	the exposed surface side	1270:1293	However, in the case of light plus water exposure, lignin degradation in the outermost cell walls proceeded from both the exposed surface side and the lumen side of the cell walls.
30145464	7	1	theme	cell	1321:1324	arg1	walls					1326:1330	the cell walls	1317:1330	the cell walls	1317:1330	However, in the case of light plus water exposure, lignin degradation in the outermost cell walls proceeded from both the exposed surface side and the lumen side of the cell walls.
30145464	0	2	theme	surfaces	74:81	arg1	composition					54:64	chemical composition	45:64	chemical composition of wood surfaces exposed to artificial weathering	45:114	Confocal Raman microscopy reveals changes in chemical composition of wood surfaces exposed to artificial weathering.
30145464	3	3	theme	high	507:510	arg1	resolution					520:529	a high spatial resolution	505:529	a high spatial resolution	505:529	A confocal Raman microscopic technique, which is useful for evaluating the structure of molecules with a high spatial resolution, was utilized to examine the effects of weathering on the chemical composition of wood surfaces at the cellular level.
30145464	0	4	theme	artificial	94:103	arg1	weathering					105:114	artificial weathering	94:114	artificial weathering	94:114	Confocal Raman microscopy reveals changes in chemical composition of wood surfaces exposed to artificial weathering.
30145464	7	5	theme	surface	1282:1288	arg1	side					1290:1293	the exposed surface side	1270:1293	the exposed surface side	1270:1293	However, in the case of light plus water exposure, lignin degradation in the outermost cell walls proceeded from both the exposed surface side and the lumen side of the cell walls.
30145464	4	6	theme	bonds	811:815	arg1	types					792:796	certain types	784:796	certain types of CC double bonds such as stilbene derivatives	784:844	Raman spectra showed that lignin degradation during weathering proceeds with the formation of o- and p-quinones, carbonyl groups, and certain types of CC double bonds such as stilbene derivatives.
30145464	4	6	theme	bonds	811:815	arg1	p-quinones					751:760	p-quinones	751:760	p-quinones	751:760	Raman spectra showed that lignin degradation during weathering proceeds with the formation of o- and p-quinones, carbonyl groups, and certain types of CC double bonds such as stilbene derivatives.
30145464	4	6	theme	bonds	811:815	arg1	derivatives					834:844	stilbene derivatives	825:844	stilbene derivatives	825:844	Raman spectra showed that lignin degradation during weathering proceeds with the formation of o- and p-quinones, carbonyl groups, and certain types of CC double bonds such as stilbene derivatives.
30145464	4	6	theme	bonds	811:815	arg1	groups					772:777	carbonyl groups	763:777	carbonyl groups	763:777	Raman spectra showed that lignin degradation during weathering proceeds with the formation of o- and p-quinones, carbonyl groups, and certain types of CC double bonds such as stilbene derivatives.
30145464	4	6	theme	bonds	811:815	arg1	bonds					811:815	CC double bonds	801:815	CC double bonds such as stilbene derivatives	801:844	Raman spectra showed that lignin degradation during weathering proceeds with the formation of o- and p-quinones, carbonyl groups, and certain types of CC double bonds such as stilbene derivatives.
30145464	4	6	theme	bonds	811:815	arg1	o-					744:745	o-	744:745	o-	744:745	Raman spectra showed that lignin degradation during weathering proceeds with the formation of o- and p-quinones, carbonyl groups, and certain types of CC double bonds such as stilbene derivatives.
30145464	4	7	theme	Raman	650:654	arg1	spectra					656:662	Raman spectra	650:662	Raman spectra	650:662	Raman spectra showed that lignin degradation during weathering proceeds with the formation of o- and p-quinones, carbonyl groups, and certain types of CC double bonds such as stilbene derivatives.
30145464	4	8	theme	p-quinones	751:760	arg1	formation					731:739	the formation	727:739	the formation of o- and p-quinones, carbonyl groups, and certain types of CC double bonds such as stilbene derivatives	727:844	Raman spectra showed that lignin degradation during weathering proceeds with the formation of o- and p-quinones, carbonyl groups, and certain types of CC double bonds such as stilbene derivatives.
30145464	2	9	from	located	344:350	arg1	layers					367:372	the surface layers	355:372	the surface layers during weathering exposure	355:399	The present study aims to assess the structural and distributional changes in the main wood polymers, such as lignin and polysaccharides, located in the surface layers during weathering exposure.
30145464	2	10	theme	wood	293:296	arg1	polymers					298:305	the main wood polymers	284:305	the main wood polymers	284:305	The present study aims to assess the structural and distributional changes in the main wood polymers, such as lignin and polysaccharides, located in the surface layers during weathering exposure.
30145464	2	10	theme	wood	293:296	arg1	polysaccharides					327:341	polysaccharides	327:341	polysaccharides	327:341	The present study aims to assess the structural and distributional changes in the main wood polymers, such as lignin and polysaccharides, located in the surface layers during weathering exposure.
30145464	2	10	theme	wood	293:296	arg1	lignin					316:321	lignin	316:321	lignin	316:321	The present study aims to assess the structural and distributional changes in the main wood polymers, such as lignin and polysaccharides, located in the surface layers during weathering exposure.
30145464	3	11	from	composition	598:608	arg1	level					643:647	the cellular level	630:647	the cellular level	630:647	A confocal Raman microscopic technique, which is useful for evaluating the structure of molecules with a high spatial resolution, was utilized to examine the effects of weathering on the chemical composition of wood surfaces at the cellular level.
30145464	5	12	theme	water	917:921	arg1	exposure					923:930	water exposure	917:930	water exposure	917:930	Comparing the weathering conditions between light only and light plus water exposure, it was found that weathering in the presence of water significantly enhances the degradation of lignin.
30145464	3	13	theme	molecules	490:498	arg1	structure					477:485	the structure	473:485	the structure of molecules	473:498	A confocal Raman microscopic technique, which is useful for evaluating the structure of molecules with a high spatial resolution, was utilized to examine the effects of weathering on the chemical composition of wood surfaces at the cellular level.
30145464	7	14	from	degradation	1210:1220	arg1	walls					1244:1248	the outermost cell walls	1225:1248	the outermost cell walls	1225:1248	However, in the case of light plus water exposure, lignin degradation in the outermost cell walls proceeded from both the exposed surface side and the lumen side of the cell walls.
30145464	2	15	theme	main	288:291	arg1	polymers					298:305	the main wood polymers	284:305	the main wood polymers	284:305	The present study aims to assess the structural and distributional changes in the main wood polymers, such as lignin and polysaccharides, located in the surface layers during weathering exposure.
30145464	2	15	theme	main	288:291	arg1	polysaccharides					327:341	polysaccharides	327:341	polysaccharides	327:341	The present study aims to assess the structural and distributional changes in the main wood polymers, such as lignin and polysaccharides, located in the surface layers during weathering exposure.
30145464	2	15	theme	main	288:291	arg1	lignin					316:321	lignin	316:321	lignin	316:321	The present study aims to assess the structural and distributional changes in the main wood polymers, such as lignin and polysaccharides, located in the surface layers during weathering exposure.
30145464	1	16	theme	components	194:203	arg1	modification					164:175	modification	164:175	modification	164:175	Weathering leads to rapid depolymerization and modification of wood chemical components.
30145464	1	16	theme	components	194:203	arg1	depolymerization					143:158	depolymerization	143:158	depolymerization	143:158	Weathering leads to rapid depolymerization and modification of wood chemical components.
30145464	1	17	theme	wood	180:183	arg1	components					194:203	wood chemical components	180:203	wood chemical components	180:203	Weathering leads to rapid depolymerization and modification of wood chemical components.
30145464	3	18	theme	confocal	404:411	arg1	technique					431:439	A confocal Raman microscopic technique	402:439	A confocal Raman microscopic technique	402:439	A confocal Raman microscopic technique, which is useful for evaluating the structure of molecules with a high spatial resolution, was utilized to examine the effects of weathering on the chemical composition of wood surfaces at the cellular level.
30145464	3	18	theme	confocal	404:411	arg1	useful					451:456	useful	451:456	useful	451:456	A confocal Raman microscopic technique, which is useful for evaluating the structure of molecules with a high spatial resolution, was utilized to examine the effects of weathering on the chemical composition of wood surfaces at the cellular level.
30145464	6	19	theme	only	1111:1114	arg1	exposure					1116:1123	light only exposure	1105:1123	light only exposure	1105:1123	The Raman images exhibited that the degree of lignin degradation by light only exposure is according to the depth.
30145464	4	20	theme	carbonyl	763:770	arg1	derivatives					834:844	stilbene derivatives	825:844	stilbene derivatives	825:844	Raman spectra showed that lignin degradation during weathering proceeds with the formation of o- and p-quinones, carbonyl groups, and certain types of CC double bonds such as stilbene derivatives.
30145464	4	20	theme	carbonyl	763:770	arg1	bonds					811:815	CC double bonds	801:815	CC double bonds such as stilbene derivatives	801:844	Raman spectra showed that lignin degradation during weathering proceeds with the formation of o- and p-quinones, carbonyl groups, and certain types of CC double bonds such as stilbene derivatives.
30145464	4	20	theme	carbonyl	763:770	arg1	groups					772:777	carbonyl groups	763:777	carbonyl groups	763:777	Raman spectra showed that lignin degradation during weathering proceeds with the formation of o- and p-quinones, carbonyl groups, and certain types of CC double bonds such as stilbene derivatives.
30145464	1	21	theme	chemical	185:192	arg1	components					194:203	wood chemical components	180:203	wood chemical components	180:203	Weathering leads to rapid depolymerization and modification of wood chemical components.
30145464	0	22	theme	Raman	9:13	arg1	microscopy					15:24	Confocal Raman microscopy	0:24	Confocal Raman microscopy	0:24	Confocal Raman microscopy reveals changes in chemical composition of wood surfaces exposed to artificial weathering.
30145464	0	23	from	changes	34:40	arg1	composition					54:64	chemical composition	45:64	chemical composition of wood surfaces exposed to artificial weathering	45:114	Confocal Raman microscopy reveals changes in chemical composition of wood surfaces exposed to artificial weathering.
30145464	7	24	theme	light	1176:1180	arg1	case					1168:1171	the case	1164:1171	the case of light plus water exposure	1164:1200	However, in the case of light plus water exposure, lignin degradation in the outermost cell walls proceeded from both the exposed surface side and the lumen side of the cell walls.
30145464	2	25	theme	weathering	381:390	arg1	exposure					392:399	weathering exposure	381:399	weathering exposure	381:399	The present study aims to assess the structural and distributional changes in the main wood polymers, such as lignin and polysaccharides, located in the surface layers during weathering exposure.
30145464	0	26	theme	Confocal	0:7	arg1	microscopy					15:24	Confocal Raman microscopy	0:24	Confocal Raman microscopy	0:24	Confocal Raman microscopy reveals changes in chemical composition of wood surfaces exposed to artificial weathering.
30145464	7	27	theme	exposure	1193:1200	arg1	case					1168:1171	the case	1164:1171	the case of light plus water exposure	1164:1200	However, in the case of light plus water exposure, lignin degradation in the outermost cell walls proceeded from both the exposed surface side and the lumen side of the cell walls.
30145464	4	28	theme	stilbene	825:832	arg1	derivatives					834:844	stilbene derivatives	825:844	stilbene derivatives	825:844	Raman spectra showed that lignin degradation during weathering proceeds with the formation of o- and p-quinones, carbonyl groups, and certain types of CC double bonds such as stilbene derivatives.
30145464	2	29	from	layers	367:372	arg1	located					344:350	located	344:350	located	344:350	The present study aims to assess the structural and distributional changes in the main wood polymers, such as lignin and polysaccharides, located in the surface layers during weathering exposure.
30145464	7	30	theme	lumen	1303:1307	arg1	side					1309:1312	the lumen side	1299:1312	the lumen side of the cell walls	1299:1330	However, in the case of light plus water exposure, lignin degradation in the outermost cell walls proceeded from both the exposed surface side and the lumen side of the cell walls.
30145464	3	31	used	utilized	536:543	arg2	technique					431:439	A confocal Raman microscopic technique	402:439	A confocal Raman microscopic technique	402:439	A confocal Raman microscopic technique, which is useful for evaluating the structure of molecules with a high spatial resolution, was utilized to examine the effects of weathering on the chemical composition of wood surfaces at the cellular level.
30145464	3	31	used	utilized	536:543	arg2	useful					451:456	useful	451:456	useful	451:456	A confocal Raman microscopic technique, which is useful for evaluating the structure of molecules with a high spatial resolution, was utilized to examine the effects of weathering on the chemical composition of wood surfaces at the cellular level.
30145464	4	32	theme	types	792:796	arg1	formation					731:739	the formation	727:739	the formation of o- and p-quinones, carbonyl groups, and certain types of CC double bonds such as stilbene derivatives	727:844	Raman spectra showed that lignin degradation during weathering proceeds with the formation of o- and p-quinones, carbonyl groups, and certain types of CC double bonds such as stilbene derivatives.
30145464	4	33	theme	CC	801:802	arg1	derivatives					834:844	stilbene derivatives	825:844	stilbene derivatives	825:844	Raman spectra showed that lignin degradation during weathering proceeds with the formation of o- and p-quinones, carbonyl groups, and certain types of CC double bonds such as stilbene derivatives.
30145464	4	33	theme	CC	801:802	arg1	bonds					811:815	CC double bonds	801:815	CC double bonds such as stilbene derivatives	801:844	Raman spectra showed that lignin degradation during weathering proceeds with the formation of o- and p-quinones, carbonyl groups, and certain types of CC double bonds such as stilbene derivatives.
30145464	6	34	theme	degradation	1090:1100	arg1	depth					1145:1149	the depth	1141:1149	the depth	1141:1149	The Raman images exhibited that the degree of lignin degradation by light only exposure is according to the depth.
30145464	6	34	theme	degradation	1090:1100	arg1	degree					1073:1078	the degree	1069:1078	the degree of lignin degradation by light only exposure	1069:1123	The Raman images exhibited that the degree of lignin degradation by light only exposure is according to the depth.
30145464	4	35	theme	groups	772:777	arg1	formation					731:739	the formation	727:739	the formation of o- and p-quinones, carbonyl groups, and certain types of CC double bonds such as stilbene derivatives	727:844	Raman spectra showed that lignin degradation during weathering proceeds with the formation of o- and p-quinones, carbonyl groups, and certain types of CC double bonds such as stilbene derivatives.
30145464	3	36	theme	cellular	634:641	arg1	level					643:647	the cellular level	630:647	the cellular level	630:647	A confocal Raman microscopic technique, which is useful for evaluating the structure of molecules with a high spatial resolution, was utilized to examine the effects of weathering on the chemical composition of wood surfaces at the cellular level.
30145464	4	37	theme	o-	744:745	arg1	formation					731:739	the formation	727:739	the formation of o- and p-quinones, carbonyl groups, and certain types of CC double bonds such as stilbene derivatives	727:844	Raman spectra showed that lignin degradation during weathering proceeds with the formation of o- and p-quinones, carbonyl groups, and certain types of CC double bonds such as stilbene derivatives.
30145464	4	38	theme	lignin	676:681	arg1	degradation					683:693	lignin degradation	676:693	lignin degradation during weathering	676:711	Raman spectra showed that lignin degradation during weathering proceeds with the formation of o- and p-quinones, carbonyl groups, and certain types of CC double bonds such as stilbene derivatives.
30145464	3	39	theme	Raman	413:417	arg1	technique					431:439	A confocal Raman microscopic technique	402:439	A confocal Raman microscopic technique	402:439	A confocal Raman microscopic technique, which is useful for evaluating the structure of molecules with a high spatial resolution, was utilized to examine the effects of weathering on the chemical composition of wood surfaces at the cellular level.
30145464	3	39	theme	Raman	413:417	arg1	useful					451:456	useful	451:456	useful	451:456	A confocal Raman microscopic technique, which is useful for evaluating the structure of molecules with a high spatial resolution, was utilized to examine the effects of weathering on the chemical composition of wood surfaces at the cellular level.
30145464	7	40	theme	cell	1239:1242	arg1	walls					1244:1248	the outermost cell walls	1225:1248	the outermost cell walls	1225:1248	However, in the case of light plus water exposure, lignin degradation in the outermost cell walls proceeded from both the exposed surface side and the lumen side of the cell walls.
30145464	7	41	theme	lignin	1203:1208	arg1	degradation					1210:1220	lignin degradation	1203:1220	lignin degradation in the outermost cell walls	1203:1248	However, in the case of light plus water exposure, lignin degradation in the outermost cell walls proceeded from both the exposed surface side and the lumen side of the cell walls.
30145464	2	42	theme	structural	243:252	arg1	changes					273:279	the structural and distributional changes	239:279	the structural and distributional changes in the main wood polymers, such as lignin and polysaccharides, located in the surface layers during weathering exposure	239:399	The present study aims to assess the structural and distributional changes in the main wood polymers, such as lignin and polysaccharides, located in the surface layers during weathering exposure.
30145464	2	43	from	changes	273:279	arg1	polymers					298:305	the main wood polymers	284:305	the main wood polymers	284:305	The present study aims to assess the structural and distributional changes in the main wood polymers, such as lignin and polysaccharides, located in the surface layers during weathering exposure.
30145464	2	43	from	changes	273:279	arg1	polysaccharides					327:341	polysaccharides	327:341	polysaccharides	327:341	The present study aims to assess the structural and distributional changes in the main wood polymers, such as lignin and polysaccharides, located in the surface layers during weathering exposure.
30145464	2	43	from	changes	273:279	arg1	lignin					316:321	lignin	316:321	lignin	316:321	The present study aims to assess the structural and distributional changes in the main wood polymers, such as lignin and polysaccharides, located in the surface layers during weathering exposure.
30145464	3	44	theme	microscopic	419:429	arg1	technique					431:439	A confocal Raman microscopic technique	402:439	A confocal Raman microscopic technique	402:439	A confocal Raman microscopic technique, which is useful for evaluating the structure of molecules with a high spatial resolution, was utilized to examine the effects of weathering on the chemical composition of wood surfaces at the cellular level.
30145464	3	44	theme	microscopic	419:429	arg1	useful					451:456	useful	451:456	useful	451:456	A confocal Raman microscopic technique, which is useful for evaluating the structure of molecules with a high spatial resolution, was utilized to examine the effects of weathering on the chemical composition of wood surfaces at the cellular level.
30145464	8	45	theme	effective	1399:1407	arg1	methods					1423:1429	more effective and efficient methods	1394:1429	more effective and efficient methods	1394:1429	This study is expected to potentially promote development of more effective and efficient methods to protect wood surfaces against weathering.
30145464	3	46	theme	chemical	589:596	arg1	composition					598:608	the chemical composition	585:608	the chemical composition of wood surfaces at the cellular level	585:647	A confocal Raman microscopic technique, which is useful for evaluating the structure of molecules with a high spatial resolution, was utilized to examine the effects of weathering on the chemical composition of wood surfaces at the cellular level.
30145464	3	47	from	level	643:647	arg1	composition					598:608	the chemical composition	585:608	the chemical composition of wood surfaces at the cellular level	585:647	A confocal Raman microscopic technique, which is useful for evaluating the structure of molecules with a high spatial resolution, was utilized to examine the effects of weathering on the chemical composition of wood surfaces at the cellular level.
30145464	3	47	from	level	643:647	arg1	surfaces					618:625	wood surfaces	613:625	wood surfaces at the cellular level	613:647	A confocal Raman microscopic technique, which is useful for evaluating the structure of molecules with a high spatial resolution, was utilized to examine the effects of weathering on the chemical composition of wood surfaces at the cellular level.
30145464	3	48	theme	weathering	571:580	arg1	effects					560:566	the effects	556:566	the effects of weathering on the chemical composition of wood surfaces at the cellular level	556:647	A confocal Raman microscopic technique, which is useful for evaluating the structure of molecules with a high spatial resolution, was utilized to examine the effects of weathering on the chemical composition of wood surfaces at the cellular level.
30145464	6	49	theme	lignin	1083:1088	arg1	degradation					1090:1100	lignin degradation	1083:1100	lignin degradation	1083:1100	The Raman images exhibited that the degree of lignin degradation by light only exposure is according to the depth.
30145464	2	50	theme	located	344:350	arg1	lignin					316:321	lignin	316:321	lignin	316:321	The present study aims to assess the structural and distributional changes in the main wood polymers, such as lignin and polysaccharides, located in the surface layers during weathering exposure.
30145464	0	51	theme	chemical	45:52	arg1	composition					54:64	chemical composition	45:64	chemical composition of wood surfaces exposed to artificial weathering	45:114	Confocal Raman microscopy reveals changes in chemical composition of wood surfaces exposed to artificial weathering.
30145464	6	52	theme	Raman	1041:1045	arg1	images					1047:1052	The Raman images	1037:1052	The Raman images	1037:1052	The Raman images exhibited that the degree of lignin degradation by light only exposure is according to the depth.
30145464	8	53	theme	efficient	1413:1421	arg1	methods					1423:1429	more effective and efficient methods	1394:1429	more effective and efficient methods	1394:1429	This study is expected to potentially promote development of more effective and efficient methods to protect wood surfaces against weathering.
30145464	4	54	theme	double	804:809	arg1	derivatives					834:844	stilbene derivatives	825:844	stilbene derivatives	825:844	Raman spectra showed that lignin degradation during weathering proceeds with the formation of o- and p-quinones, carbonyl groups, and certain types of CC double bonds such as stilbene derivatives.
30145464	4	54	theme	double	804:809	arg1	bonds					811:815	CC double bonds	801:815	CC double bonds such as stilbene derivatives	801:844	Raman spectra showed that lignin degradation during weathering proceeds with the formation of o- and p-quinones, carbonyl groups, and certain types of CC double bonds such as stilbene derivatives.
30145464	7	55	theme	outermost	1229:1237	arg1	walls					1244:1248	the outermost cell walls	1225:1248	the outermost cell walls	1225:1248	However, in the case of light plus water exposure, lignin degradation in the outermost cell walls proceeded from both the exposed surface side and the lumen side of the cell walls.
30145464	6	56	theme	light	1105:1109	arg1	exposure					1116:1123	light only exposure	1105:1123	light only exposure	1105:1123	The Raman images exhibited that the degree of lignin degradation by light only exposure is according to the depth.
30145464	5	57	theme	water	981:985	arg1	presence					969:976	the presence	965:976	the presence of water	965:985	Comparing the weathering conditions between light only and light plus water exposure, it was found that weathering in the presence of water significantly enhances the degradation of lignin.
30145464	5	58	theme	lignin	1029:1034	arg1	degradation					1014:1024	the degradation	1010:1024	the degradation of lignin	1010:1034	Comparing the weathering conditions between light only and light plus water exposure, it was found that weathering in the presence of water significantly enhances the degradation of lignin.
30145464	3	59	theme	wood	613:616	arg1	surfaces					618:625	wood surfaces	613:625	wood surfaces at the cellular level	613:647	A confocal Raman microscopic technique, which is useful for evaluating the structure of molecules with a high spatial resolution, was utilized to examine the effects of weathering on the chemical composition of wood surfaces at the cellular level.
30145464	7	60	theme	walls	1326:1330	arg1	side					1309:1312	the lumen side	1299:1312	the lumen side of the cell walls	1299:1330	However, in the case of light plus water exposure, lignin degradation in the outermost cell walls proceeded from both the exposed surface side and the lumen side of the cell walls.
30145464	7	60	theme	walls	1326:1330	arg1	side					1290:1293	the exposed surface side	1270:1293	the exposed surface side	1270:1293	However, in the case of light plus water exposure, lignin degradation in the outermost cell walls proceeded from both the exposed surface side and the lumen side of the cell walls.
30145464	5	61	theme	weathering	861:870	arg1	conditions					872:881	the weathering conditions	857:881	the weathering conditions	857:881	Comparing the weathering conditions between light only and light plus water exposure, it was found that weathering in the presence of water significantly enhances the degradation of lignin.
30145464	2	62	theme	present	210:216	arg1	study					218:222	The present study	206:222	The present study	206:222	The present study aims to assess the structural and distributional changes in the main wood polymers, such as lignin and polysaccharides, located in the surface layers during weathering exposure.
30145464	3	63	theme	surfaces	618:625	arg1	composition					598:608	the chemical composition	585:608	the chemical composition of wood surfaces at the cellular level	585:647	A confocal Raman microscopic technique, which is useful for evaluating the structure of molecules with a high spatial resolution, was utilized to examine the effects of weathering on the chemical composition of wood surfaces at the cellular level.
30145464	1	64	theme	rapid	137:141	arg1	depolymerization					143:158	depolymerization	143:158	depolymerization	143:158	Weathering leads to rapid depolymerization and modification of wood chemical components.
30145464	3	65	from	effects	560:566	arg1	composition					598:608	the chemical composition	585:608	the chemical composition of wood surfaces at the cellular level	585:647	A confocal Raman microscopic technique, which is useful for evaluating the structure of molecules with a high spatial resolution, was utilized to examine the effects of weathering on the chemical composition of wood surfaces at the cellular level.
30145464	0	66	theme	wood	69:72	arg1	surfaces					74:81	wood surfaces	69:81	wood surfaces exposed to artificial weathering	69:114	Confocal Raman microscopy reveals changes in chemical composition of wood surfaces exposed to artificial weathering.
30145464	8	67	theme	methods	1423:1429	arg1	development					1379:1389	development	1379:1389	development of more effective and efficient methods to protect wood surfaces against weathering	1379:1473	This study is expected to potentially promote development of more effective and efficient methods to protect wood surfaces against weathering.
30145464	2	68	theme	distributional	258:271	arg1	changes					273:279	the structural and distributional changes	239:279	the structural and distributional changes in the main wood polymers, such as lignin and polysaccharides, located in the surface layers during weathering exposure	239:399	The present study aims to assess the structural and distributional changes in the main wood polymers, such as lignin and polysaccharides, located in the surface layers during weathering exposure.
30145464	3	69	theme	spatial	512:518	arg1	resolution					520:529	a high spatial resolution	505:529	a high spatial resolution	505:529	A confocal Raman microscopic technique, which is useful for evaluating the structure of molecules with a high spatial resolution, was utilized to examine the effects of weathering on the chemical composition of wood surfaces at the cellular level.
30145464	7	70	theme	water	1187:1191	arg1	exposure					1193:1200	water exposure	1187:1200	water exposure	1187:1200	However, in the case of light plus water exposure, lignin degradation in the outermost cell walls proceeded from both the exposed surface side and the lumen side of the cell walls.
30145464	8	71	theme	wood	1442:1445	arg1	surfaces					1447:1454	wood surfaces	1442:1454	wood surfaces	1442:1454	This study is expected to potentially promote development of more effective and efficient methods to protect wood surfaces against weathering.
30145464	4	72	theme	certain	784:790	arg1	types					792:796	certain types	784:796	certain types of CC double bonds such as stilbene derivatives	784:844	Raman spectra showed that lignin degradation during weathering proceeds with the formation of o- and p-quinones, carbonyl groups, and certain types of CC double bonds such as stilbene derivatives.
30145464	2	73	theme	surface	359:365	arg1	layers					367:372	the surface layers	355:372	the surface layers during weathering exposure	355:399	The present study aims to assess the structural and distributional changes in the main wood polymers, such as lignin and polysaccharides, located in the surface layers during weathering exposure.
30145464	5	74	from	weathering	951:960	arg1	presence					969:976	the presence	965:976	the presence of water	965:985	Comparing the weathering conditions between light only and light plus water exposure, it was found that weathering in the presence of water significantly enhances the degradation of lignin.
30986033	0	0	from	Components	15:24	arg1	Hosts					73:77	Arthropod and Vertebrate Hosts	48:77	Hosts	73:77	Dissecting the Components of Sindbis Virus from Arthropod and Vertebrate Hosts: Implications for Infectivity Differences.
30986033	4	1	theme	mass	502:505	arg1	spectrometry					507:518	charge detection mass spectrometry	485:518	charge detection mass spectrometry	485:518	In this study, charge detection mass spectrometry was used to determine the masses of intact SINV particles isolated from BHK and C6/36 cells.
30986033	5	2	theme	derived	680:686	arg1	SINV					688:691	BHK derived SINV	676:691	BHK derived SINV	676:691	The measured masses are substantially different: 52.88 MDa for BHK derived SINV and 50.69 MDa for C6/36 derived.
30986033	6	3	theme	derived	835:841	arg1	SINV					843:846	BHK derived SINV	831:846	BHK derived SINV	831:846	Further analysis using several mass spectrometry-based methods and biophysical approaches indicates that BHK derived SINV has a substantially higher mass than C6/36 derived because in the lipid bilayer, there is a higher portion of lipids containing long chain fatty acids.
30986033	4	4	attach	isolated	578:585	arg1	cells					606:610	BHK and C6/36 cells	592:610	cells	606:610	In this study, charge detection mass spectrometry was used to determine the masses of intact SINV particles isolated from BHK and C6/36 cells.
30986033	4	4	attach	isolated	578:585	arg2	particles					568:576	intact SINV particles	556:576	intact SINV particles isolated from BHK and C6/36 cells	556:610	In this study, charge detection mass spectrometry was used to determine the masses of intact SINV particles isolated from BHK and C6/36 cells.
30986033	6	5	theme	spectrometry-based	762:779	arg1	methods					781:787	several mass spectrometry-based methods	749:787	several mass spectrometry-based methods	749:787	Further analysis using several mass spectrometry-based methods and biophysical approaches indicates that BHK derived SINV has a substantially higher mass than C6/36 derived because in the lipid bilayer, there is a higher portion of lipids containing long chain fatty acids.
30986033	1	6	theme	wide	227:230	arg1	range					232:236	a wide range	225:236	a wide range of vertebrate hosts	225:256	Sindbis virus (SINV) is an enveloped, single-stranded RNA virus, which is transmitted via mosquitos to a wide range of vertebrate hosts.
30986033	7	7	theme	lipid	1018:1022	arg1	composition					1024:1034	lipid composition	1018:1034	lipid composition	1018:1034	The difference in lipid composition could influence the organization of the lipid bilayer.
30986033	8	8	theme	fusion	1235:1240	arg1	events					1242:1247	fusion events	1235:1247	fusion events	1235:1247	As a result, multiple stages of the viral lifecycle may be affected including assembly and budding, particle stability during transmission, and fusion events, all of which could contribute to the differences in infectivity.
30986033	4	9	theme	SINV	563:566	arg1	particles					568:576	intact SINV particles	556:576	intact SINV particles isolated from BHK and C6/36 cells	556:610	In this study, charge detection mass spectrometry was used to determine the masses of intact SINV particles isolated from BHK and C6/36 cells.
30986033	6	10	theme	fatty	987:991	arg1	acids					993:997	long chain fatty acids	976:997	long chain fatty acids	976:997	Further analysis using several mass spectrometry-based methods and biophysical approaches indicates that BHK derived SINV has a substantially higher mass than C6/36 derived because in the lipid bilayer, there is a higher portion of lipids containing long chain fatty acids.
30986033	4	11	theme	BHK	592:594	arg1	cells					606:610	BHK and C6/36 cells	592:610	cells	606:610	In this study, charge detection mass spectrometry was used to determine the masses of intact SINV particles isolated from BHK and C6/36 cells.
30986033	1	12	theme	enveloped	149:157	arg1	virus					130:134	Sindbis virus	122:134	Sindbis virus (SINV)	122:141	Sindbis virus (SINV) is an enveloped, single-stranded RNA virus, which is transmitted via mosquitos to a wide range of vertebrate hosts.
30986033	1	12	theme	enveloped	149:157	arg1	virus					180:184	an enveloped, single-stranded RNA virus	146:184	virus	180:184	Sindbis virus (SINV) is an enveloped, single-stranded RNA virus, which is transmitted via mosquitos to a wide range of vertebrate hosts.
30986033	0	13	theme	Infectivity	97:107	arg1	Differences					109:119	Infectivity Differences	97:119	Infectivity Differences	97:119	Dissecting the Components of Sindbis Virus from Arthropod and Vertebrate Hosts: Implications for Infectivity Differences.
30986033	6	14	dep	derived	835:841	arg1	BHK					831:833	BHK	831:833	BHK	831:833	Further analysis using several mass spectrometry-based methods and biophysical approaches indicates that BHK derived SINV has a substantially higher mass than C6/36 derived because in the lipid bilayer, there is a higher portion of lipids containing long chain fatty acids.
30986033	2	15	dep	cells	314:318	arg1	kidney					301:306	baby hamster kidney	288:306	baby hamster kidney (BHK)	288:312	SINV produced by vertebrate, baby hamster kidney (BHK) cells is more than an order of magnitude less infectious than SINV produced from mosquito (C6/36) cells.
30986033	2	15	dep	cells	314:318	arg1	BHK					309:311	BHK	309:311	BHK	309:311	SINV produced by vertebrate, baby hamster kidney (BHK) cells is more than an order of magnitude less infectious than SINV produced from mosquito (C6/36) cells.
30986033	4	16	theme	particles	568:576	arg1	masses					546:551	the masses	542:551	the masses of intact SINV particles isolated from BHK and C6/36 cells	542:610	In this study, charge detection mass spectrometry was used to determine the masses of intact SINV particles isolated from BHK and C6/36 cells.
30986033	7	17	theme	bilayer	1082:1088	arg1	organization					1056:1067	the organization	1052:1067	the organization of the lipid bilayer	1052:1088	The difference in lipid composition could influence the organization of the lipid bilayer.
30986033	6	18	theme	biophysical	793:803	arg1	approaches					805:814	biophysical approaches	793:814	biophysical approaches	793:814	Further analysis using several mass spectrometry-based methods and biophysical approaches indicates that BHK derived SINV has a substantially higher mass than C6/36 derived because in the lipid bilayer, there is a higher portion of lipids containing long chain fatty acids.
30986033	5	19	dep	derived	680:686	arg1	BHK					676:678	BHK	676:678	BHK	676:678	The measured masses are substantially different: 52.88 MDa for BHK derived SINV and 50.69 MDa for C6/36 derived.
30986033	6	20	theme	long	976:979	arg1	acids					993:997	long chain fatty acids	976:997	long chain fatty acids	976:997	Further analysis using several mass spectrometry-based methods and biophysical approaches indicates that BHK derived SINV has a substantially higher mass than C6/36 derived because in the lipid bilayer, there is a higher portion of lipids containing long chain fatty acids.
30986033	1	21	theme	single-stranded	160:174	arg1	virus					130:134	Sindbis virus	122:134	Sindbis virus (SINV)	122:141	Sindbis virus (SINV) is an enveloped, single-stranded RNA virus, which is transmitted via mosquitos to a wide range of vertebrate hosts.
30986033	1	21	theme	single-stranded	160:174	arg1	virus					180:184	an enveloped, single-stranded RNA virus	146:184	virus	180:184	Sindbis virus (SINV) is an enveloped, single-stranded RNA virus, which is transmitted via mosquitos to a wide range of vertebrate hosts.
30986033	1	22	theme	vertebrate	241:250	arg1	hosts					252:256	vertebrate hosts	241:256	vertebrate hosts	241:256	Sindbis virus (SINV) is an enveloped, single-stranded RNA virus, which is transmitted via mosquitos to a wide range of vertebrate hosts.
30986033	6	23	theme	Further	726:732	arg1	analysis					734:741	Further analysis	726:741	Further analysis using several mass spectrometry-based methods and biophysical approaches	726:814	Further analysis using several mass spectrometry-based methods and biophysical approaches indicates that BHK derived SINV has a substantially higher mass than C6/36 derived because in the lipid bilayer, there is a higher portion of lipids containing long chain fatty acids.
30986033	2	24	theme	vertebrate	276:285	arg1	cells					314:318	vertebrate, baby hamster kidney (BHK) cells	276:318	cells	314:318	SINV produced by vertebrate, baby hamster kidney (BHK) cells is more than an order of magnitude less infectious than SINV produced from mosquito (C6/36) cells.
30986033	8	25	from	differences	1287:1297	arg1	infectivity					1302:1312	infectivity	1302:1312	infectivity	1302:1312	As a result, multiple stages of the viral lifecycle may be affected including assembly and budding, particle stability during transmission, and fusion events, all of which could contribute to the differences in infectivity.
30986033	1	26	theme	RNA	176:178	arg1	virus					130:134	Sindbis virus	122:134	Sindbis virus (SINV)	122:141	Sindbis virus (SINV) is an enveloped, single-stranded RNA virus, which is transmitted via mosquitos to a wide range of vertebrate hosts.
30986033	1	26	theme	RNA	176:178	arg1	virus					180:184	an enveloped, single-stranded RNA virus	146:184	virus	180:184	Sindbis virus (SINV) is an enveloped, single-stranded RNA virus, which is transmitted via mosquitos to a wide range of vertebrate hosts.
30986033	1	27	theme	hosts	252:256	arg1	range					232:236	a wide range	225:236	a wide range of vertebrate hosts	225:256	Sindbis virus (SINV) is an enveloped, single-stranded RNA virus, which is transmitted via mosquitos to a wide range of vertebrate hosts.
30986033	8	28	theme	particle	1191:1198	arg1	stability					1200:1208	particle stability	1191:1208	particle stability during transmission	1191:1228	As a result, multiple stages of the viral lifecycle may be affected including assembly and budding, particle stability during transmission, and fusion events, all of which could contribute to the differences in infectivity.
30986033	6	29	theme	lipids	958:963	arg1	portion					947:953	a higher portion	938:953	a higher portion of lipids containing long chain fatty acids	938:997	Further analysis using several mass spectrometry-based methods and biophysical approaches indicates that BHK derived SINV has a substantially higher mass than C6/36 derived because in the lipid bilayer, there is a higher portion of lipids containing long chain fatty acids.
30986033	5	30	link	derived	680:686	arg1	SINV					688:691	BHK derived SINV	676:691	BHK derived SINV	676:691	The measured masses are substantially different: 52.88 MDa for BHK derived SINV and 50.69 MDa for C6/36 derived.
30986033	4	31	theme	charge	485:490	arg1	spectrometry					507:518	charge detection mass spectrometry	485:518	charge detection mass spectrometry	485:518	In this study, charge detection mass spectrometry was used to determine the masses of intact SINV particles isolated from BHK and C6/36 cells.
30986033	5	32	theme	measured	617:624	arg1	MDa					703:705	50.69 MDa	697:705	50.69 MDa for C6/36 derived	697:723	The measured masses are substantially different: 52.88 MDa for BHK derived SINV and 50.69 MDa for C6/36 derived.
30986033	5	32	theme	measured	617:624	arg1	masses					626:631	The measured masses	613:631	The measured masses	613:631	The measured masses are substantially different: 52.88 MDa for BHK derived SINV and 50.69 MDa for C6/36 derived.
30986033	5	32	theme	measured	617:624	arg1	different					651:659	different	651:659	different	651:659	The measured masses are substantially different: 52.88 MDa for BHK derived SINV and 50.69 MDa for C6/36 derived.
30986033	5	32	theme	measured	617:624	arg1	MDa					668:670	52.88 MDa	662:670	52.88 MDa for BHK derived SINV	662:691	The measured masses are substantially different: 52.88 MDa for BHK derived SINV and 50.69 MDa for C6/36 derived.
30986033	4	33	theme	C6/36	600:604	arg1	cells					606:610	BHK and C6/36 cells	592:610	cells	606:610	In this study, charge detection mass spectrometry was used to determine the masses of intact SINV particles isolated from BHK and C6/36 cells.
30986033	0	34	theme	Virus	37:41	arg1	Components					15:24	the Components	11:24	the Components of Sindbis Virus from Arthropod and Vertebrate Hosts	11:77	Dissecting the Components of Sindbis Virus from Arthropod and Vertebrate Hosts: Implications for Infectivity Differences.
30986033	7	35	from	difference	1004:1013	arg1	composition					1024:1034	lipid composition	1018:1034	lipid composition	1018:1034	The difference in lipid composition could influence the organization of the lipid bilayer.
30986033	4	36	used	used	524:527	arg2	spectrometry					507:518	charge detection mass spectrometry	485:518	charge detection mass spectrometry	485:518	In this study, charge detection mass spectrometry was used to determine the masses of intact SINV particles isolated from BHK and C6/36 cells.
30986033	0	37	from	Hosts	73:77	arg1	Components					15:24	the Components	11:24	the Components of Sindbis Virus from Arthropod and Vertebrate Hosts	11:77	Dissecting the Components of Sindbis Virus from Arthropod and Vertebrate Hosts: Implications for Infectivity Differences.
30986033	0	37	from	Hosts	73:77	arg1	Virus					37:41	Sindbis Virus	29:41	Sindbis Virus from Arthropod and Vertebrate Hosts	29:77	Dissecting the Components of Sindbis Virus from Arthropod and Vertebrate Hosts: Implications for Infectivity Differences.
30986033	6	38	contain	has	848:850	arg2	mass					875:878	a substantially higher mass	852:878	a substantially higher mass than C6/36 derived because in the lipid bilayer, there is a higher portion of lipids containing long chain fatty acids	852:997	Further analysis using several mass spectrometry-based methods and biophysical approaches indicates that BHK derived SINV has a substantially higher mass than C6/36 derived because in the lipid bilayer, there is a higher portion of lipids containing long chain fatty acids.
30986033	6	38	contain	has	848:850	arg1	SINV					843:846	BHK derived SINV	831:846	BHK derived SINV	831:846	Further analysis using several mass spectrometry-based methods and biophysical approaches indicates that BHK derived SINV has a substantially higher mass than C6/36 derived because in the lipid bilayer, there is a higher portion of lipids containing long chain fatty acids.
30986033	2	39	theme	magnitude	345:353	arg1	order					336:340	an order	333:340	an order of magnitude	333:353	SINV produced by vertebrate, baby hamster kidney (BHK) cells is more than an order of magnitude less infectious than SINV produced from mosquito (C6/36) cells.
30986033	0	40	theme	Sindbis	29:35	arg1	Virus					37:41	Sindbis Virus	29:41	Sindbis Virus from Arthropod and Vertebrate Hosts	29:77	Dissecting the Components of Sindbis Virus from Arthropod and Vertebrate Hosts: Implications for Infectivity Differences.
30986033	4	41	theme	detection	492:500	arg1	spectrometry					507:518	charge detection mass spectrometry	485:518	charge detection mass spectrometry	485:518	In this study, charge detection mass spectrometry was used to determine the masses of intact SINV particles isolated from BHK and C6/36 cells.
30986033	6	42	theme	lipid	914:918	arg1	bilayer					920:926	the lipid bilayer	910:926	the lipid bilayer	910:926	Further analysis using several mass spectrometry-based methods and biophysical approaches indicates that BHK derived SINV has a substantially higher mass than C6/36 derived because in the lipid bilayer, there is a higher portion of lipids containing long chain fatty acids.
30986033	4	43	theme	intact	556:561	arg1	particles					568:576	intact SINV particles	556:576	intact SINV particles isolated from BHK and C6/36 cells	556:610	In this study, charge detection mass spectrometry was used to determine the masses of intact SINV particles isolated from BHK and C6/36 cells.
30986033	0	44	theme	Arthropod	48:56	arg1	Hosts					73:77	Arthropod and Vertebrate Hosts	48:77	Hosts	73:77	Dissecting the Components of Sindbis Virus from Arthropod and Vertebrate Hosts: Implications for Infectivity Differences.
30986033	6	45	theme	higher	868:873	arg1	mass					875:878	a substantially higher mass	852:878	a substantially higher mass than C6/36 derived because in the lipid bilayer, there is a higher portion of lipids containing long chain fatty acids	852:997	Further analysis using several mass spectrometry-based methods and biophysical approaches indicates that BHK derived SINV has a substantially higher mass than C6/36 derived because in the lipid bilayer, there is a higher portion of lipids containing long chain fatty acids.
30986033	8	46	theme	multiple	1104:1111	arg1	result					1096:1101	a result	1094:1101	a result	1094:1101	As a result, multiple stages of the viral lifecycle may be affected including assembly and budding, particle stability during transmission, and fusion events, all of which could contribute to the differences in infectivity.
30986033	8	46	theme	multiple	1104:1111	arg1	stages					1113:1118	multiple stages	1104:1118	multiple stages of the viral lifecycle	1104:1141	As a result, multiple stages of the viral lifecycle may be affected including assembly and budding, particle stability during transmission, and fusion events, all of which could contribute to the differences in infectivity.
30986033	6	47	theme	mass	757:760	arg1	methods					781:787	several mass spectrometry-based methods	749:787	several mass spectrometry-based methods	749:787	Further analysis using several mass spectrometry-based methods and biophysical approaches indicates that BHK derived SINV has a substantially higher mass than C6/36 derived because in the lipid bilayer, there is a higher portion of lipids containing long chain fatty acids.
30986033	2	48	theme	mosquito	395:402	arg1	cells					412:416	mosquito (C6/36) cells	395:416	mosquito (C6/36) cells	395:416	SINV produced by vertebrate, baby hamster kidney (BHK) cells is more than an order of magnitude less infectious than SINV produced from mosquito (C6/36) cells.
30986033	6	49	theme	several	749:755	arg1	methods					781:787	several mass spectrometry-based methods	749:787	several mass spectrometry-based methods	749:787	Further analysis using several mass spectrometry-based methods and biophysical approaches indicates that BHK derived SINV has a substantially higher mass than C6/36 derived because in the lipid bilayer, there is a higher portion of lipids containing long chain fatty acids.
30986033	1	50	theme	Sindbis	122:128	arg1	virus					130:134	Sindbis virus	122:134	Sindbis virus (SINV)	122:141	Sindbis virus (SINV) is an enveloped, single-stranded RNA virus, which is transmitted via mosquitos to a wide range of vertebrate hosts.
30986033	1	50	theme	Sindbis	122:128	arg1	virus					180:184	an enveloped, single-stranded RNA virus	146:184	virus	180:184	Sindbis virus (SINV) is an enveloped, single-stranded RNA virus, which is transmitted via mosquitos to a wide range of vertebrate hosts.
30986033	1	50	theme	Sindbis	122:128	arg1	SINV					137:140	SINV	137:140	SINV	137:140	Sindbis virus (SINV) is an enveloped, single-stranded RNA virus, which is transmitted via mosquitos to a wide range of vertebrate hosts.
30986033	5	51	link	derived	717:723	arg1	C6/36					711:715	C6/36	711:715	C6/36	711:715	The measured masses are substantially different: 52.88 MDa for BHK derived SINV and 50.69 MDa for C6/36 derived.
30986033	2	52	theme	hamster	293:299	arg1	kidney					301:306	baby hamster kidney	288:306	baby hamster kidney (BHK)	288:312	SINV produced by vertebrate, baby hamster kidney (BHK) cells is more than an order of magnitude less infectious than SINV produced from mosquito (C6/36) cells.
30986033	2	52	theme	hamster	293:299	arg1	BHK					309:311	BHK	309:311	BHK	309:311	SINV produced by vertebrate, baby hamster kidney (BHK) cells is more than an order of magnitude less infectious than SINV produced from mosquito (C6/36) cells.
30986033	5	53	dep	different	651:659	arg1	MDa					703:705	50.69 MDa	697:705	50.69 MDa for C6/36 derived	697:723	The measured masses are substantially different: 52.88 MDa for BHK derived SINV and 50.69 MDa for C6/36 derived.
30986033	5	53	dep	different	651:659	arg1	masses					626:631	The measured masses	613:631	The measured masses	613:631	The measured masses are substantially different: 52.88 MDa for BHK derived SINV and 50.69 MDa for C6/36 derived.
30986033	5	53	dep	different	651:659	arg1	different					651:659	different	651:659	different	651:659	The measured masses are substantially different: 52.88 MDa for BHK derived SINV and 50.69 MDa for C6/36 derived.
30986033	5	53	dep	different	651:659	arg1	MDa					668:670	52.88 MDa	662:670	52.88 MDa for BHK derived SINV	662:691	The measured masses are substantially different: 52.88 MDa for BHK derived SINV and 50.69 MDa for C6/36 derived.
30986033	8	54	theme	viral	1127:1131	arg1	lifecycle					1133:1141	the viral lifecycle	1123:1141	the viral lifecycle	1123:1141	As a result, multiple stages of the viral lifecycle may be affected including assembly and budding, particle stability during transmission, and fusion events, all of which could contribute to the differences in infectivity.
30986033	2	55	dep	more	323:326	arg1	infectious					360:369	infectious	360:369	infectious	360:369	SINV produced by vertebrate, baby hamster kidney (BHK) cells is more than an order of magnitude less infectious than SINV produced from mosquito (C6/36) cells.
30986033	8	56	theme	lifecycle	1133:1141	arg1	result					1096:1101	a result	1094:1101	a result	1094:1101	As a result, multiple stages of the viral lifecycle may be affected including assembly and budding, particle stability during transmission, and fusion events, all of which could contribute to the differences in infectivity.
30986033	8	56	theme	lifecycle	1133:1141	arg1	stages					1113:1118	multiple stages	1104:1118	multiple stages of the viral lifecycle	1104:1141	As a result, multiple stages of the viral lifecycle may be affected including assembly and budding, particle stability during transmission, and fusion events, all of which could contribute to the differences in infectivity.
30986033	2	57	theme	baby	288:291	arg1	kidney					301:306	baby hamster kidney	288:306	baby hamster kidney (BHK)	288:312	SINV produced by vertebrate, baby hamster kidney (BHK) cells is more than an order of magnitude less infectious than SINV produced from mosquito (C6/36) cells.
30986033	2	57	theme	baby	288:291	arg1	BHK					309:311	BHK	309:311	BHK	309:311	SINV produced by vertebrate, baby hamster kidney (BHK) cells is more than an order of magnitude less infectious than SINV produced from mosquito (C6/36) cells.
30986033	0	58	theme	Vertebrate	62:71	arg1	Hosts					73:77	Arthropod and Vertebrate Hosts	48:77	Hosts	73:77	Dissecting the Components of Sindbis Virus from Arthropod and Vertebrate Hosts: Implications for Infectivity Differences.
30986033	6	59	theme	higher	940:945	arg1	portion					947:953	a higher portion	938:953	a higher portion of lipids containing long chain fatty acids	938:997	Further analysis using several mass spectrometry-based methods and biophysical approaches indicates that BHK derived SINV has a substantially higher mass than C6/36 derived because in the lipid bilayer, there is a higher portion of lipids containing long chain fatty acids.
30986033	6	60	link	derived	835:841	arg1	SINV					843:846	BHK derived SINV	831:846	BHK derived SINV	831:846	Further analysis using several mass spectrometry-based methods and biophysical approaches indicates that BHK derived SINV has a substantially higher mass than C6/36 derived because in the lipid bilayer, there is a higher portion of lipids containing long chain fatty acids.
30986033	3	61	theme	difference	437:446	arg1	cause					423:427	The cause	419:427	The cause of this difference	419:446	The cause of this difference is poorly understood.
30986033	6	62	theme	chain	981:985	arg1	acids					993:997	long chain fatty acids	976:997	long chain fatty acids	976:997	Further analysis using several mass spectrometry-based methods and biophysical approaches indicates that BHK derived SINV has a substantially higher mass than C6/36 derived because in the lipid bilayer, there is a higher portion of lipids containing long chain fatty acids.
30986033	6	63	contain	containing	965:974	arg2	acids					993:997	long chain fatty acids	976:997	long chain fatty acids	976:997	Further analysis using several mass spectrometry-based methods and biophysical approaches indicates that BHK derived SINV has a substantially higher mass than C6/36 derived because in the lipid bilayer, there is a higher portion of lipids containing long chain fatty acids.
30986033	6	63	contain	containing	965:974	arg1	lipids					958:963	lipids	958:963	lipids containing long chain fatty acids	958:997	Further analysis using several mass spectrometry-based methods and biophysical approaches indicates that BHK derived SINV has a substantially higher mass than C6/36 derived because in the lipid bilayer, there is a higher portion of lipids containing long chain fatty acids.
30986033	0	64	dep	Dissecting	0:9	arg1	Implications					80:91	Implications	80:91	Implications for Infectivity Differences	80:119	Dissecting the Components of Sindbis Virus from Arthropod and Vertebrate Hosts: Implications for Infectivity Differences.
30986033	7	65	theme	lipid	1076:1080	arg1	bilayer					1082:1088	the lipid bilayer	1072:1088	the lipid bilayer	1072:1088	The difference in lipid composition could influence the organization of the lipid bilayer.
30852321	6	0	theme	sugars	1072:1077	arg1	decrease					980:987	a decrease	978:987	a decrease in carbohydrates	978:1004	FT-IR analysis shows upon selective removal of sialic acids a decrease in carbohydrates, extension of the protein side chain, and exposure of penultimate sugars.
30852321	6	0	theme	sugars	1072:1077	arg1	exposure					1048:1055	exposure	1048:1055	exposure of penultimate sugars	1048:1077	FT-IR analysis shows upon selective removal of sialic acids a decrease in carbohydrates, extension of the protein side chain, and exposure of penultimate sugars.
30852321	6	0	theme	sugars	1072:1077	arg1	extension					1007:1015	extension	1007:1015	extension of the protein side chain	1007:1041	FT-IR analysis shows upon selective removal of sialic acids a decrease in carbohydrates, extension of the protein side chain, and exposure of penultimate sugars.
30852321	7	1	theme	acids	1108:1112	arg1	removal					1090:1096	Enzymatic removal	1080:1096	Enzymatic removal of sialic acids	1080:1112	Enzymatic removal of sialic acids results in the removal of galactose residues from the EPS upon subsequent treatment with β-galactosidase, indicating a linkage between galactose and sialic acid at the terminus of glycan chains.
30852321	8	2	theme	sialic	1347:1352	arg1	acids					1354:1358	sialic acids	1347:1358	sialic acids	1347:1358	This work indicates the importance of sialic acids in the protection of penultimate sugar residues of glycoproteins in EPS, and provides basis for future research in the composition of EPS from biofilms and granular sludge.
30852321	3	3	from	role	502:505	arg1	AGS					529:531	AGS	529:531	AGS	529:531	In order to further understand the role of these compounds in AGS, lectin staining, genome analysis of the dominant bacterial species, and shielding tests were done.
30852321	8	4	gly	glycoproteins	1411:1423	arg1	glycoproteins					1411:1423	glycoproteins	1411:1423	glycoproteins in EPS	1411:1430	This work indicates the importance of sialic acids in the protection of penultimate sugar residues of glycoproteins in EPS, and provides basis for future research in the composition of EPS from biofilms and granular sludge.
30852321	7	5	theme	Enzymatic	1080:1088	arg1	removal					1090:1096	Enzymatic removal	1080:1096	Enzymatic removal of sialic acids	1080:1112	Enzymatic removal of sialic acids results in the removal of galactose residues from the EPS upon subsequent treatment with β-galactosidase, indicating a linkage between galactose and sialic acid at the terminus of glycan chains.
30852321	3	6	theme	compounds	516:524	arg1	role					502:505	the role	498:505	the role of these compounds in AGS	498:531	In order to further understand the role of these compounds in AGS, lectin staining, genome analysis of the dominant bacterial species, and shielding tests were done.
30852321	4	7	theme	lectin	646:651	arg1	analysis					671:678	Fluorescence lectin bar-coding (FLBC) analysis	633:678	Fluorescence lectin bar-coding (FLBC) analysis	633:678	Fluorescence lectin bar-coding (FLBC) analysis showed an overlap with protein staining, indicating presence of sialoglycoproteins in the EPS matrix.
30852321	8	8	from	glycoproteins	1411:1423	arg1	EPS					1428:1430	EPS	1428:1430	EPS	1428:1430	This work indicates the importance of sialic acids in the protection of penultimate sugar residues of glycoproteins in EPS, and provides basis for future research in the composition of EPS from biofilms and granular sludge.
30852321	1	9	theme	seawater-adapted	185:200	arg1	AGS					227:229	AGS	227:229	AGS	227:229	Sialic acids have been discovered in the extracellular polymeric substances (EPS) of seawater-adapted aerobic granular sludge (AGS).
30852321	1	9	theme	seawater-adapted	185:200	arg1	sludge					219:224	seawater-adapted aerobic granular sludge	185:224	seawater-adapted aerobic granular sludge (AGS)	185:230	Sialic acids have been discovered in the extracellular polymeric substances (EPS) of seawater-adapted aerobic granular sludge (AGS).
30852321	3	10	theme	lectin	534:539	arg1	staining					541:548	lectin staining	534:548	lectin staining	534:548	In order to further understand the role of these compounds in AGS, lectin staining, genome analysis of the dominant bacterial species, and shielding tests were done.
30852321	8	11	from	residues	1399:1406	arg1	EPS					1428:1430	EPS	1428:1430	EPS	1428:1430	This work indicates the importance of sialic acids in the protection of penultimate sugar residues of glycoproteins in EPS, and provides basis for future research in the composition of EPS from biofilms and granular sludge.
30852321	5	12	theme	positive	806:813	arg1	indication					815:824	a positive indication	804:824	a positive indication for putative production of sialic acids by the dominant bacteria Candidatus Accumulibacter	804:915	Genome analysis gives a positive indication for putative production of sialic acids by the dominant bacteria Candidatus Accumulibacter.
30852321	6	13	theme	selective	944:952	arg1	removal					954:960	selective removal	944:960	selective removal of sialic acids	944:976	FT-IR analysis shows upon selective removal of sialic acids a decrease in carbohydrates, extension of the protein side chain, and exposure of penultimate sugars.
30852321	7	14	from	EPS	1168:1170	arg1	removal					1129:1135	the removal	1125:1135	the removal of galactose residues from the EPS upon subsequent treatment with β-galactosidase	1125:1217	Enzymatic removal of sialic acids results in the removal of galactose residues from the EPS upon subsequent treatment with β-galactosidase, indicating a linkage between galactose and sialic acid at the terminus of glycan chains.
30852321	6	15	theme	FT-IR	918:922	arg1	analysis					924:931	FT-IR analysis	918:931	FT-IR analysis	918:931	FT-IR analysis shows upon selective removal of sialic acids a decrease in carbohydrates, extension of the protein side chain, and exposure of penultimate sugars.
30852321	8	16	from	sludge	1525:1530	arg1	EPS					1494:1496	EPS	1494:1496	EPS from biofilms and granular sludge	1494:1530	This work indicates the importance of sialic acids in the protection of penultimate sugar residues of glycoproteins in EPS, and provides basis for future research in the composition of EPS from biofilms and granular sludge.
30852321	8	16	from	sludge	1525:1530	arg1	composition					1479:1489	the composition	1475:1489	the composition of EPS from biofilms and granular sludge	1475:1530	This work indicates the importance of sialic acids in the protection of penultimate sugar residues of glycoproteins in EPS, and provides basis for future research in the composition of EPS from biofilms and granular sludge.
30852321	7	17	with	treatment	1188:1196	arg1	β-galactosidase					1203:1217	β-galactosidase	1203:1217	β-galactosidase	1203:1217	Enzymatic removal of sialic acids results in the removal of galactose residues from the EPS upon subsequent treatment with β-galactosidase, indicating a linkage between galactose and sialic acid at the terminus of glycan chains.
30852321	0	18	theme	seawater-adapted	58:73	arg1	sludge					92:97	seawater-adapted aerobic granular sludge	58:97	seawater-adapted aerobic granular sludge	58:97	Sialic acids in the extracellular polymeric substances of seawater-adapted aerobic granular sludge.
30852321	7	19	theme	galactose	1140:1148	arg1	residues					1150:1157	galactose residues	1140:1157	galactose residues	1140:1157	Enzymatic removal of sialic acids results in the removal of galactose residues from the EPS upon subsequent treatment with β-galactosidase, indicating a linkage between galactose and sialic acid at the terminus of glycan chains.
30852321	1	20	theme	polymeric	155:163	arg1	EPS					177:179	EPS	177:179	EPS	177:179	Sialic acids have been discovered in the extracellular polymeric substances (EPS) of seawater-adapted aerobic granular sludge (AGS).
30852321	1	20	theme	polymeric	155:163	arg1	substances					165:174	the extracellular polymeric substances	137:174	the extracellular polymeric substances (EPS) of seawater-adapted aerobic granular sludge (AGS)	137:230	Sialic acids have been discovered in the extracellular polymeric substances (EPS) of seawater-adapted aerobic granular sludge (AGS).
30852321	0	21	theme	granular	83:90	arg1	sludge					92:97	seawater-adapted aerobic granular sludge	58:97	seawater-adapted aerobic granular sludge	58:97	Sialic acids in the extracellular polymeric substances of seawater-adapted aerobic granular sludge.
30852321	5	22	theme	dominant	873:880	arg1	bacteria					882:889	the dominant bacteria	869:889	the dominant bacteria Candidatus Accumulibacter	869:915	Genome analysis gives a positive indication for putative production of sialic acids by the dominant bacteria Candidatus Accumulibacter.
30852321	7	23	theme	subsequent	1177:1186	arg1	treatment					1188:1196	subsequent treatment	1177:1196	subsequent treatment with β-galactosidase	1177:1217	Enzymatic removal of sialic acids results in the removal of galactose residues from the EPS upon subsequent treatment with β-galactosidase, indicating a linkage between galactose and sialic acid at the terminus of glycan chains.
30852321	4	24	from	presence	732:739	arg1	matrix					774:779	the EPS matrix	766:779	the EPS matrix	766:779	Fluorescence lectin bar-coding (FLBC) analysis showed an overlap with protein staining, indicating presence of sialoglycoproteins in the EPS matrix.
30852321	6	25	theme	acids	972:976	arg1	removal					954:960	selective removal	944:960	selective removal of sialic acids	944:976	FT-IR analysis shows upon selective removal of sialic acids a decrease in carbohydrates, extension of the protein side chain, and exposure of penultimate sugars.
30852321	5	26	dep	bacteria	882:889	arg1	Accumulibacter					902:915	Accumulibacter	902:915	Accumulibacter	902:915	Genome analysis gives a positive indication for putative production of sialic acids by the dominant bacteria Candidatus Accumulibacter.
30852321	0	27	theme	polymeric	34:42	arg1	substances					44:53	the extracellular polymeric substances	16:53	the extracellular polymeric substances of seawater-adapted aerobic granular sludge	16:97	Sialic acids in the extracellular polymeric substances of seawater-adapted aerobic granular sludge.
30852321	4	28	theme	protein	703:709	arg1	staining					711:718	protein staining	703:718	protein staining	703:718	Fluorescence lectin bar-coding (FLBC) analysis showed an overlap with protein staining, indicating presence of sialoglycoproteins in the EPS matrix.
30852321	5	29	theme	Genome	782:787	arg1	analysis					789:796	Genome analysis	782:796	Genome analysis	782:796	Genome analysis gives a positive indication for putative production of sialic acids by the dominant bacteria Candidatus Accumulibacter.
30852321	1	30	theme	Sialic	100:105	arg1	acids					107:111	Sialic acids	100:111	Sialic acids	100:111	Sialic acids have been discovered in the extracellular polymeric substances (EPS) of seawater-adapted aerobic granular sludge (AGS).
30852321	7	31	theme	chains	1301:1306	arg1	terminus					1282:1289	the terminus	1278:1289	the terminus of glycan chains	1278:1306	Enzymatic removal of sialic acids results in the removal of galactose residues from the EPS upon subsequent treatment with β-galactosidase, indicating a linkage between galactose and sialic acid at the terminus of glycan chains.
30852321	8	32	from	research	1463:1470	arg1	composition					1479:1489	the composition	1475:1489	the composition of EPS from biofilms and granular sludge	1475:1530	This work indicates the importance of sialic acids in the protection of penultimate sugar residues of glycoproteins in EPS, and provides basis for future research in the composition of EPS from biofilms and granular sludge.
30852321	6	33	theme	side	1032:1035	arg1	chain					1037:1041	the protein side chain	1020:1041	the protein side chain	1020:1041	FT-IR analysis shows upon selective removal of sialic acids a decrease in carbohydrates, extension of the protein side chain, and exposure of penultimate sugars.
30852321	2	34	theme	EPS	401:403	arg1	molecules					405:413	EPS molecules	401:413	EPS molecules	401:413	Sialic acids are a group of monosaccharides with a nine-carbon backbone, commonly found in mammalian cells and pathogenic bacteria, and frequently described to protect EPS molecules and cells from attack by proteases or glycosidases.
30852321	0	35	theme	extracellular	20:32	arg1	substances					44:53	the extracellular polymeric substances	16:53	the extracellular polymeric substances of seawater-adapted aerobic granular sludge	16:97	Sialic acids in the extracellular polymeric substances of seawater-adapted aerobic granular sludge.
30852321	3	36	theme	shielding	606:614	arg1	tests					616:620	shielding tests	606:620	shielding tests	606:620	In order to further understand the role of these compounds in AGS, lectin staining, genome analysis of the dominant bacterial species, and shielding tests were done.
30852321	8	37	theme	penultimate	1381:1391	arg1	residues					1399:1406	penultimate sugar residues	1381:1406	penultimate sugar residues of glycoproteins in EPS	1381:1430	This work indicates the importance of sialic acids in the protection of penultimate sugar residues of glycoproteins in EPS, and provides basis for future research in the composition of EPS from biofilms and granular sludge.
30852321	8	37	theme	penultimate	1381:1391	arg1	glycoproteins					1411:1423	glycoproteins	1411:1423	glycoproteins in EPS	1411:1430	This work indicates the importance of sialic acids in the protection of penultimate sugar residues of glycoproteins in EPS, and provides basis for future research in the composition of EPS from biofilms and granular sludge.
30852321	3	38	theme	bacterial	583:591	arg1	species					593:599	the dominant bacterial species	570:599	the dominant bacterial species	570:599	In order to further understand the role of these compounds in AGS, lectin staining, genome analysis of the dominant bacterial species, and shielding tests were done.
30852321	8	39	theme	residues	1399:1406	arg1	protection					1367:1376	the protection	1363:1376	the protection of penultimate sugar residues of glycoproteins in EPS	1363:1430	This work indicates the importance of sialic acids in the protection of penultimate sugar residues of glycoproteins in EPS, and provides basis for future research in the composition of EPS from biofilms and granular sludge.
30852321	8	40	theme	glycoproteins	1411:1423	arg1	residues					1399:1406	penultimate sugar residues	1381:1406	penultimate sugar residues of glycoproteins in EPS	1381:1430	This work indicates the importance of sialic acids in the protection of penultimate sugar residues of glycoproteins in EPS, and provides basis for future research in the composition of EPS from biofilms and granular sludge.
30852321	8	40	theme	glycoproteins	1411:1423	arg1	glycoproteins					1411:1423	glycoproteins	1411:1423	glycoproteins in EPS	1411:1430	This work indicates the importance of sialic acids in the protection of penultimate sugar residues of glycoproteins in EPS, and provides basis for future research in the composition of EPS from biofilms and granular sludge.
30852321	8	41	from	EPS	1428:1430	arg1	residues					1399:1406	penultimate sugar residues	1381:1406	penultimate sugar residues of glycoproteins in EPS	1381:1430	This work indicates the importance of sialic acids in the protection of penultimate sugar residues of glycoproteins in EPS, and provides basis for future research in the composition of EPS from biofilms and granular sludge.
30852321	8	41	from	EPS	1428:1430	arg1	glycoproteins					1411:1423	glycoproteins	1411:1423	glycoproteins in EPS	1411:1430	This work indicates the importance of sialic acids in the protection of penultimate sugar residues of glycoproteins in EPS, and provides basis for future research in the composition of EPS from biofilms and granular sludge.
30852321	7	42	theme	sialic	1101:1106	arg1	acids					1108:1112	sialic acids	1101:1112	sialic acids	1101:1112	Enzymatic removal of sialic acids results in the removal of galactose residues from the EPS upon subsequent treatment with β-galactosidase, indicating a linkage between galactose and sialic acid at the terminus of glycan chains.
30852321	2	43	theme	pathogenic	344:353	arg1	bacteria					355:362	pathogenic bacteria	344:362	pathogenic bacteria	344:362	Sialic acids are a group of monosaccharides with a nine-carbon backbone, commonly found in mammalian cells and pathogenic bacteria, and frequently described to protect EPS molecules and cells from attack by proteases or glycosidases.
30852321	6	44	theme	penultimate	1060:1070	arg1	sugars					1072:1077	penultimate sugars	1060:1077	penultimate sugars	1060:1077	FT-IR analysis shows upon selective removal of sialic acids a decrease in carbohydrates, extension of the protein side chain, and exposure of penultimate sugars.
30852321	6	45	from	exposure	1048:1055	arg1	carbohydrates					992:1004	carbohydrates	992:1004	carbohydrates	992:1004	FT-IR analysis shows upon selective removal of sialic acids a decrease in carbohydrates, extension of the protein side chain, and exposure of penultimate sugars.
30852321	2	46	theme	monosaccharides	261:275	arg1	monosaccharides					261:275	monosaccharides	261:275	monosaccharides	261:275	Sialic acids are a group of monosaccharides with a nine-carbon backbone, commonly found in mammalian cells and pathogenic bacteria, and frequently described to protect EPS molecules and cells from attack by proteases or glycosidases.
30852321	2	46	theme	monosaccharides	261:275	arg1	acids					240:244	Sialic acids	233:244	Sialic acids	233:244	Sialic acids are a group of monosaccharides with a nine-carbon backbone, commonly found in mammalian cells and pathogenic bacteria, and frequently described to protect EPS molecules and cells from attack by proteases or glycosidases.
30852321	2	46	theme	monosaccharides	261:275	arg1	group					252:256	a group	250:256	a group of monosaccharides	250:275	Sialic acids are a group of monosaccharides with a nine-carbon backbone, commonly found in mammalian cells and pathogenic bacteria, and frequently described to protect EPS molecules and cells from attack by proteases or glycosidases.
30852321	2	47	dep	group	252:256	arg1	described					380:388	described	380:388	described to protect EPS molecules and cells from attack by proteases or glycosidases	380:464	Sialic acids are a group of monosaccharides with a nine-carbon backbone, commonly found in mammalian cells and pathogenic bacteria, and frequently described to protect EPS molecules and cells from attack by proteases or glycosidases.
30852321	2	47	dep	group	252:256	arg1	found					315:319	found	315:319	found in mammalian cells and pathogenic bacteria	315:362	Sialic acids are a group of monosaccharides with a nine-carbon backbone, commonly found in mammalian cells and pathogenic bacteria, and frequently described to protect EPS molecules and cells from attack by proteases or glycosidases.
30852321	5	48	theme	putative	830:837	arg1	production					839:848	putative production	830:848	putative production of sialic acids by the dominant bacteria Candidatus Accumulibacter	830:915	Genome analysis gives a positive indication for putative production of sialic acids by the dominant bacteria Candidatus Accumulibacter.
30852321	4	49	gly	sialoglycoproteins	744:761	arg1	sialoglycoproteins					744:761	sialoglycoproteins	744:761	sialoglycoproteins	744:761	Fluorescence lectin bar-coding (FLBC) analysis showed an overlap with protein staining, indicating presence of sialoglycoproteins in the EPS matrix.
30852321	6	50	theme	sialic	965:970	arg1	acids					972:976	sialic acids	965:976	sialic acids	965:976	FT-IR analysis shows upon selective removal of sialic acids a decrease in carbohydrates, extension of the protein side chain, and exposure of penultimate sugars.
30852321	4	51	theme	Fluorescence	633:644	arg1	analysis					671:678	Fluorescence lectin bar-coding (FLBC) analysis	633:678	Fluorescence lectin bar-coding (FLBC) analysis	633:678	Fluorescence lectin bar-coding (FLBC) analysis showed an overlap with protein staining, indicating presence of sialoglycoproteins in the EPS matrix.
30852321	4	52	attach	presence	732:739	arg2	sialoglycoproteins					744:761	sialoglycoproteins	744:761	sialoglycoproteins	744:761	Fluorescence lectin bar-coding (FLBC) analysis showed an overlap with protein staining, indicating presence of sialoglycoproteins in the EPS matrix.
30852321	4	52	attach	presence	732:739	arg1	matrix					774:779	the EPS matrix	766:779	the EPS matrix	766:779	Fluorescence lectin bar-coding (FLBC) analysis showed an overlap with protein staining, indicating presence of sialoglycoproteins in the EPS matrix.
30852321	1	53	theme	aerobic	202:208	arg1	AGS					227:229	AGS	227:229	AGS	227:229	Sialic acids have been discovered in the extracellular polymeric substances (EPS) of seawater-adapted aerobic granular sludge (AGS).
30852321	1	53	theme	aerobic	202:208	arg1	sludge					219:224	seawater-adapted aerobic granular sludge	185:224	seawater-adapted aerobic granular sludge (AGS)	185:230	Sialic acids have been discovered in the extracellular polymeric substances (EPS) of seawater-adapted aerobic granular sludge (AGS).
30852321	1	54	theme	extracellular	141:153	arg1	EPS					177:179	EPS	177:179	EPS	177:179	Sialic acids have been discovered in the extracellular polymeric substances (EPS) of seawater-adapted aerobic granular sludge (AGS).
30852321	1	54	theme	extracellular	141:153	arg1	substances					165:174	the extracellular polymeric substances	137:174	the extracellular polymeric substances (EPS) of seawater-adapted aerobic granular sludge (AGS)	137:230	Sialic acids have been discovered in the extracellular polymeric substances (EPS) of seawater-adapted aerobic granular sludge (AGS).
30852321	2	55	theme	Sialic	233:238	arg1	monosaccharides					261:275	monosaccharides	261:275	monosaccharides	261:275	Sialic acids are a group of monosaccharides with a nine-carbon backbone, commonly found in mammalian cells and pathogenic bacteria, and frequently described to protect EPS molecules and cells from attack by proteases or glycosidases.
30852321	2	55	theme	Sialic	233:238	arg1	acids					240:244	Sialic acids	233:244	Sialic acids	233:244	Sialic acids are a group of monosaccharides with a nine-carbon backbone, commonly found in mammalian cells and pathogenic bacteria, and frequently described to protect EPS molecules and cells from attack by proteases or glycosidases.
30852321	2	55	theme	Sialic	233:238	arg1	group					252:256	a group	250:256	a group of monosaccharides	250:275	Sialic acids are a group of monosaccharides with a nine-carbon backbone, commonly found in mammalian cells and pathogenic bacteria, and frequently described to protect EPS molecules and cells from attack by proteases or glycosidases.
30852321	5	56	theme	sialic	853:858	arg1	acids					860:864	sialic acids	853:864	sialic acids	853:864	Genome analysis gives a positive indication for putative production of sialic acids by the dominant bacteria Candidatus Accumulibacter.
30852321	1	57	theme	sludge	219:224	arg1	EPS					177:179	EPS	177:179	EPS	177:179	Sialic acids have been discovered in the extracellular polymeric substances (EPS) of seawater-adapted aerobic granular sludge (AGS).
30852321	1	57	theme	sludge	219:224	arg1	substances					165:174	the extracellular polymeric substances	137:174	the extracellular polymeric substances (EPS) of seawater-adapted aerobic granular sludge (AGS)	137:230	Sialic acids have been discovered in the extracellular polymeric substances (EPS) of seawater-adapted aerobic granular sludge (AGS).
30852321	3	58	theme	genome	551:556	arg1	analysis					558:565	genome analysis	551:565	genome analysis of the dominant bacterial species	551:599	In order to further understand the role of these compounds in AGS, lectin staining, genome analysis of the dominant bacterial species, and shielding tests were done.
30852321	2	59	with	group	252:256	arg1	backbone					296:303	a nine-carbon backbone	282:303	a nine-carbon backbone	282:303	Sialic acids are a group of monosaccharides with a nine-carbon backbone, commonly found in mammalian cells and pathogenic bacteria, and frequently described to protect EPS molecules and cells from attack by proteases or glycosidases.
30852321	3	60	theme	species	593:599	arg1	tests					616:620	shielding tests	606:620	shielding tests	606:620	In order to further understand the role of these compounds in AGS, lectin staining, genome analysis of the dominant bacterial species, and shielding tests were done.
30852321	3	60	theme	species	593:599	arg1	staining					541:548	lectin staining	534:548	lectin staining	534:548	In order to further understand the role of these compounds in AGS, lectin staining, genome analysis of the dominant bacterial species, and shielding tests were done.
30852321	3	60	theme	species	593:599	arg1	analysis					558:565	genome analysis	551:565	genome analysis of the dominant bacterial species	551:599	In order to further understand the role of these compounds in AGS, lectin staining, genome analysis of the dominant bacterial species, and shielding tests were done.
30852321	8	61	theme	future	1456:1461	arg1	research					1463:1470	future research	1456:1470	future research in the composition of EPS from biofilms and granular sludge	1456:1530	This work indicates the importance of sialic acids in the protection of penultimate sugar residues of glycoproteins in EPS, and provides basis for future research in the composition of EPS from biofilms and granular sludge.
30852321	0	62	theme	aerobic	75:81	arg1	sludge					92:97	seawater-adapted aerobic granular sludge	58:97	seawater-adapted aerobic granular sludge	58:97	Sialic acids in the extracellular polymeric substances of seawater-adapted aerobic granular sludge.
30852321	3	63	theme	dominant	574:581	arg1	species					593:599	the dominant bacterial species	570:599	the dominant bacterial species	570:599	In order to further understand the role of these compounds in AGS, lectin staining, genome analysis of the dominant bacterial species, and shielding tests were done.
30852321	5	64	theme	acids	860:864	arg1	production					839:848	putative production	830:848	putative production of sialic acids by the dominant bacteria Candidatus Accumulibacter	830:915	Genome analysis gives a positive indication for putative production of sialic acids by the dominant bacteria Candidatus Accumulibacter.
30852321	4	65	theme	bar-coding	653:662	arg1	analysis					671:678	Fluorescence lectin bar-coding (FLBC) analysis	633:678	Fluorescence lectin bar-coding (FLBC) analysis	633:678	Fluorescence lectin bar-coding (FLBC) analysis showed an overlap with protein staining, indicating presence of sialoglycoproteins in the EPS matrix.
30852321	0	66	theme	sludge	92:97	arg1	substances					44:53	the extracellular polymeric substances	16:53	the extracellular polymeric substances of seawater-adapted aerobic granular sludge	16:97	Sialic acids in the extracellular polymeric substances of seawater-adapted aerobic granular sludge.
30852321	0	67	from	acids	7:11	arg1	substances					44:53	the extracellular polymeric substances	16:53	the extracellular polymeric substances of seawater-adapted aerobic granular sludge	16:97	Sialic acids in the extracellular polymeric substances of seawater-adapted aerobic granular sludge.
30852321	4	68	theme	FLBC	665:668	arg1	analysis					671:678	Fluorescence lectin bar-coding (FLBC) analysis	633:678	Fluorescence lectin bar-coding (FLBC) analysis	633:678	Fluorescence lectin bar-coding (FLBC) analysis showed an overlap with protein staining, indicating presence of sialoglycoproteins in the EPS matrix.
30852321	2	69	theme	nine-carbon	284:294	arg1	backbone					296:303	a nine-carbon backbone	282:303	a nine-carbon backbone	282:303	Sialic acids are a group of monosaccharides with a nine-carbon backbone, commonly found in mammalian cells and pathogenic bacteria, and frequently described to protect EPS molecules and cells from attack by proteases or glycosidases.
30852321	8	70	theme	EPS	1494:1496	arg1	composition					1479:1489	the composition	1475:1489	the composition of EPS from biofilms and granular sludge	1475:1530	This work indicates the importance of sialic acids in the protection of penultimate sugar residues of glycoproteins in EPS, and provides basis for future research in the composition of EPS from biofilms and granular sludge.
30852321	6	71	from	extension	1007:1015	arg1	carbohydrates					992:1004	carbohydrates	992:1004	carbohydrates	992:1004	FT-IR analysis shows upon selective removal of sialic acids a decrease in carbohydrates, extension of the protein side chain, and exposure of penultimate sugars.
30852321	0	72	theme	Sialic	0:5	arg1	acids					7:11	Sialic acids	0:11	Sialic acids in the extracellular polymeric substances of seawater-adapted aerobic granular sludge	0:97	Sialic acids in the extracellular polymeric substances of seawater-adapted aerobic granular sludge.
30852321	1	73	theme	granular	210:217	arg1	AGS					227:229	AGS	227:229	AGS	227:229	Sialic acids have been discovered in the extracellular polymeric substances (EPS) of seawater-adapted aerobic granular sludge (AGS).
30852321	1	73	theme	granular	210:217	arg1	sludge					219:224	seawater-adapted aerobic granular sludge	185:224	seawater-adapted aerobic granular sludge (AGS)	185:230	Sialic acids have been discovered in the extracellular polymeric substances (EPS) of seawater-adapted aerobic granular sludge (AGS).
30852321	7	74	theme	residues	1150:1157	arg1	removal					1129:1135	the removal	1125:1135	the removal of galactose residues from the EPS upon subsequent treatment with β-galactosidase	1125:1217	Enzymatic removal of sialic acids results in the removal of galactose residues from the EPS upon subsequent treatment with β-galactosidase, indicating a linkage between galactose and sialic acid at the terminus of glycan chains.
30852321	3	75	dep	understand	487:496	arg1	to					476:477	to	476:477	to	476:477	In order to further understand the role of these compounds in AGS, lectin staining, genome analysis of the dominant bacterial species, and shielding tests were done.
30852321	7	76	theme	glycan	1294:1299	arg1	chains					1301:1306	glycan chains	1294:1306	glycan chains	1294:1306	Enzymatic removal of sialic acids results in the removal of galactose residues from the EPS upon subsequent treatment with β-galactosidase, indicating a linkage between galactose and sialic acid at the terminus of glycan chains.
30852321	8	77	theme	granular	1516:1523	arg1	sludge					1525:1530	granular sludge	1516:1530	granular sludge	1516:1530	This work indicates the importance of sialic acids in the protection of penultimate sugar residues of glycoproteins in EPS, and provides basis for future research in the composition of EPS from biofilms and granular sludge.
30852321	6	78	from	decrease	980:987	arg1	carbohydrates					992:1004	carbohydrates	992:1004	carbohydrates	992:1004	FT-IR analysis shows upon selective removal of sialic acids a decrease in carbohydrates, extension of the protein side chain, and exposure of penultimate sugars.
30852321	6	79	theme	chain	1037:1041	arg1	decrease					980:987	a decrease	978:987	a decrease in carbohydrates	978:1004	FT-IR analysis shows upon selective removal of sialic acids a decrease in carbohydrates, extension of the protein side chain, and exposure of penultimate sugars.
30852321	6	79	theme	chain	1037:1041	arg1	exposure					1048:1055	exposure	1048:1055	exposure of penultimate sugars	1048:1077	FT-IR analysis shows upon selective removal of sialic acids a decrease in carbohydrates, extension of the protein side chain, and exposure of penultimate sugars.
30852321	6	79	theme	chain	1037:1041	arg1	extension					1007:1015	extension	1007:1015	extension of the protein side chain	1007:1041	FT-IR analysis shows upon selective removal of sialic acids a decrease in carbohydrates, extension of the protein side chain, and exposure of penultimate sugars.
30852321	4	80	theme	sialoglycoproteins	744:761	arg1	presence					732:739	presence	732:739	presence of sialoglycoproteins in the EPS matrix	732:779	Fluorescence lectin bar-coding (FLBC) analysis showed an overlap with protein staining, indicating presence of sialoglycoproteins in the EPS matrix.
30852321	8	81	theme	acids	1354:1358	arg1	importance					1333:1342	the importance	1329:1342	the importance of sialic acids in the protection of penultimate sugar residues of glycoproteins in EPS	1329:1430	This work indicates the importance of sialic acids in the protection of penultimate sugar residues of glycoproteins in EPS, and provides basis for future research in the composition of EPS from biofilms and granular sludge.
30852321	8	82	from	biofilms	1503:1510	arg1	EPS					1494:1496	EPS	1494:1496	EPS from biofilms and granular sludge	1494:1530	This work indicates the importance of sialic acids in the protection of penultimate sugar residues of glycoproteins in EPS, and provides basis for future research in the composition of EPS from biofilms and granular sludge.
30852321	8	82	from	biofilms	1503:1510	arg1	composition					1479:1489	the composition	1475:1489	the composition of EPS from biofilms and granular sludge	1475:1530	This work indicates the importance of sialic acids in the protection of penultimate sugar residues of glycoproteins in EPS, and provides basis for future research in the composition of EPS from biofilms and granular sludge.
30852321	6	83	theme	protein	1024:1030	arg1	chain					1037:1041	the protein side chain	1020:1041	the protein side chain	1020:1041	FT-IR analysis shows upon selective removal of sialic acids a decrease in carbohydrates, extension of the protein side chain, and exposure of penultimate sugars.
30852321	7	84	theme	sialic	1263:1268	arg1	acid					1270:1273	sialic acid	1263:1273	sialic acid	1263:1273	Enzymatic removal of sialic acids results in the removal of galactose residues from the EPS upon subsequent treatment with β-galactosidase, indicating a linkage between galactose and sialic acid at the terminus of glycan chains.
30852321	2	85	theme	mammalian	324:332	arg1	cells					334:338	mammalian cells	324:338	mammalian cells	324:338	Sialic acids are a group of monosaccharides with a nine-carbon backbone, commonly found in mammalian cells and pathogenic bacteria, and frequently described to protect EPS molecules and cells from attack by proteases or glycosidases.
30852321	8	86	from	importance	1333:1342	arg1	protection					1367:1376	the protection	1363:1376	the protection of penultimate sugar residues of glycoproteins in EPS	1363:1430	This work indicates the importance of sialic acids in the protection of penultimate sugar residues of glycoproteins in EPS, and provides basis for future research in the composition of EPS from biofilms and granular sludge.
30852321	8	87	theme	sugar	1393:1397	arg1	residues					1399:1406	penultimate sugar residues	1381:1406	penultimate sugar residues of glycoproteins in EPS	1381:1430	This work indicates the importance of sialic acids in the protection of penultimate sugar residues of glycoproteins in EPS, and provides basis for future research in the composition of EPS from biofilms and granular sludge.
30852321	8	87	theme	sugar	1393:1397	arg1	glycoproteins					1411:1423	glycoproteins	1411:1423	glycoproteins in EPS	1411:1430	This work indicates the importance of sialic acids in the protection of penultimate sugar residues of glycoproteins in EPS, and provides basis for future research in the composition of EPS from biofilms and granular sludge.
30852321	4	88	theme	EPS	770:772	arg1	matrix					774:779	the EPS matrix	766:779	the EPS matrix	766:779	Fluorescence lectin bar-coding (FLBC) analysis showed an overlap with protein staining, indicating presence of sialoglycoproteins in the EPS matrix.
30852321	8	89	from	composition	1479:1489	arg1	sludge					1525:1530	granular sludge	1516:1530	granular sludge	1516:1530	This work indicates the importance of sialic acids in the protection of penultimate sugar residues of glycoproteins in EPS, and provides basis for future research in the composition of EPS from biofilms and granular sludge.
30852321	8	89	from	composition	1479:1489	arg1	biofilms					1503:1510	biofilms	1503:1510	biofilms	1503:1510	This work indicates the importance of sialic acids in the protection of penultimate sugar residues of glycoproteins in EPS, and provides basis for future research in the composition of EPS from biofilms and granular sludge.
30343042	3	0	theme	rat	518:520	arg1	striatum					522:529	the rat striatum	514:529	the rat striatum	514:529	We also evaluated the expression of dopamine D2 receptors in the rat striatum.
30343042	9	1	theme	rat	1409:1411	arg1	striatum					1413:1420	the rat striatum	1405:1420	the rat striatum	1405:1420	Finally, we report that only ad libitum high-sucrose evoked a significant enhancement of the dopamine D2 receptor protein level and a reduction in the D2 mRNA and receptor affinity in the rat striatum.
30343042	8	2	theme	high-fat	1179:1186	arg1	diet					1215:1218	intermitted high-fat or continuous high-sucrose diet	1167:1218	intermitted high-fat or continuous high-sucrose diet	1167:1218	High-fat diet schedules resulted in the enhancement of leptin concentrations, while increases in blood levels of ghrelin were noted after intermitted high-fat or continuous high-sucrose diet.
30343042	5	3	dep	RESULTS	778:784	arg1	Both					786:789	Both	786:789	Both	786:789	RESULTS Both diets resulted in an enhancement in caloric intake and total body weight.
30343042	4	4	theme	ad	660:661	arg1	access					671:676	ad libitum access	660:676	ad libitum access	660:676	METHODS Male Wistar rats were fed with either high-fat or high-sucrose diet for 5 weeks according to different feeding regimes: ad libitum access or scheduled for a 2-h period each day without caloric restriction during the remainder of the day.
30343042	5	5	theme	total	846:850	arg1	weight					857:862	total body weight	846:862	total body weight	846:862	RESULTS Both diets resulted in an enhancement in caloric intake and total body weight.
30343042	8	6	theme	continuous	1191:1200	arg1	high-sucrose					1202:1213	continuous high-sucrose	1191:1213	continuous high-sucrose	1191:1213	High-fat diet schedules resulted in the enhancement of leptin concentrations, while increases in blood levels of ghrelin were noted after intermitted high-fat or continuous high-sucrose diet.
30343042	11	7	dep	CONCLUSION	1596:1605	arg1	provide					1620:1626	provide	1620:1626	provide a better understanding of eating disorders	1620:1669	CONCLUSION The findings provide a better understanding of eating disorders and indicate that diet composition leading to obesity induces distinct changes in dopamine D2 receptor signaling in the striatum.
30343042	11	7	dep	CONCLUSION	1596:1605	arg1	indicate					1675:1682	indicate	1675:1682	indicate that diet composition leading to obesity induces distinct changes in dopamine D2 receptor signaling in the striatum	1675:1798	CONCLUSION The findings provide a better understanding of eating disorders and indicate that diet composition leading to obesity induces distinct changes in dopamine D2 receptor signaling in the striatum.
30343042	10	8	theme	rats	1560:1563	arg1	striatum					1548:1555	the striatum	1544:1555	the striatum of rats with intermittent food access	1544:1593	Independently of the diet type, a similar reduction in dopamine D2 receptor affinity (decrease in KD value) was found in the striatum of rats with intermittent food access.
30343042	1	9	theme	global	195:200	arg1	proportions					211:221	global epidemic proportions	195:221	global epidemic proportions	195:221	BACKGROUND Obesity has reached global epidemic proportions and is associated with serious medical comorbidities and economic consequences.
30343042	4	10	theme	different	633:641	arg1	regimes					651:657	different feeding regimes	633:657	different feeding regimes	633:657	METHODS Male Wistar rats were fed with either high-fat or high-sucrose diet for 5 weeks according to different feeding regimes: ad libitum access or scheduled for a 2-h period each day without caloric restriction during the remainder of the day.
30343042	11	11	theme	receptor	1765:1772	arg1	signaling					1774:1782	dopamine D2 receptor signaling	1753:1782	dopamine D2 receptor signaling in the striatum	1753:1798	CONCLUSION The findings provide a better understanding of eating disorders and indicate that diet composition leading to obesity induces distinct changes in dopamine D2 receptor signaling in the striatum.
30343042	0	12	theme	high-fat	125:132	arg1	diet					150:153	continuous or scheduled high-fat or high-sucrose diet	101:153	continuous or scheduled high-fat or high-sucrose diet	101:153	Assessment of metabolic and hormonal profiles and striatal dopamine D2 receptor expression following continuous or scheduled high-fat or high-sucrose diet in rats.
30343042	6	13	theme	glucose	947:953	arg1	concentrations					955:968	glucose concentrations	947:968	glucose concentrations	947:968	Post-meal data showed that high-fat diet increased cholesterol, triglycerides and glucose concentrations.
30343042	0	14	theme	high-sucrose	137:148	arg1	diet					150:153	continuous or scheduled high-fat or high-sucrose diet	101:153	continuous or scheduled high-fat or high-sucrose diet	101:153	Assessment of metabolic and hormonal profiles and striatal dopamine D2 receptor expression following continuous or scheduled high-fat or high-sucrose diet in rats.
30343042	10	15	theme	intermittent	1570:1581	arg1	access					1588:1593	intermittent food access	1570:1593	intermittent food access	1570:1593	Independently of the diet type, a similar reduction in dopamine D2 receptor affinity (decrease in KD value) was found in the striatum of rats with intermittent food access.
30343042	9	16	theme	libitum	1253:1259	arg1	high-sucrose					1261:1272	only ad libitum high-sucrose	1245:1272	only ad libitum high-sucrose	1245:1272	Finally, we report that only ad libitum high-sucrose evoked a significant enhancement of the dopamine D2 receptor protein level and a reduction in the D2 mRNA and receptor affinity in the rat striatum.
30343042	5	17	from	enhancement	812:822	arg1	intake					835:840	caloric intake	827:840	caloric intake	827:840	RESULTS Both diets resulted in an enhancement in caloric intake and total body weight.
30343042	5	17	from	enhancement	812:822	arg1	weight					857:862	total body weight	846:862	total body weight	846:862	RESULTS Both diets resulted in an enhancement in caloric intake and total body weight.
30343042	7	18	theme	high	986:989	arg1	diet					999:1002	high sucrose diet	986:1002	high sucrose diet	986:1002	Animals fed on high sucrose diet were only hyperglycemic.
30343042	10	19	from	reduction	1465:1473	arg1	affinity					1499:1506	dopamine D2 receptor affinity	1478:1506	dopamine D2 receptor affinity (decrease in KD value)	1478:1529	Independently of the diet type, a similar reduction in dopamine D2 receptor affinity (decrease in KD value) was found in the striatum of rats with intermittent food access.
30343042	0	20	theme	dopamine	59:66	arg1	expression					80:89	striatal dopamine D2 receptor expression	50:89	striatal dopamine D2 receptor expression	50:89	Assessment of metabolic and hormonal profiles and striatal dopamine D2 receptor expression following continuous or scheduled high-fat or high-sucrose diet in rats.
30343042	9	21	theme	significant	1283:1293	arg1	enhancement					1295:1305	a significant enhancement	1281:1305	a significant enhancement of the dopamine D2 receptor protein level	1281:1347	Finally, we report that only ad libitum high-sucrose evoked a significant enhancement of the dopamine D2 receptor protein level and a reduction in the D2 mRNA and receptor affinity in the rat striatum.
30343042	10	22	theme	diet	1444:1447	arg1	type					1449:1452	the diet type	1440:1452	the diet type	1440:1452	Independently of the diet type, a similar reduction in dopamine D2 receptor affinity (decrease in KD value) was found in the striatum of rats with intermittent food access.
30343042	11	23	theme	dopamine	1753:1760	arg1	signaling					1774:1782	dopamine D2 receptor signaling	1753:1782	dopamine D2 receptor signaling in the striatum	1753:1798	CONCLUSION The findings provide a better understanding of eating disorders and indicate that diet composition leading to obesity induces distinct changes in dopamine D2 receptor signaling in the striatum.
30343042	0	24	theme	receptor	71:78	arg1	expression					80:89	striatal dopamine D2 receptor expression	50:89	striatal dopamine D2 receptor expression	50:89	Assessment of metabolic and hormonal profiles and striatal dopamine D2 receptor expression following continuous or scheduled high-fat or high-sucrose diet in rats.
30343042	1	25	theme	BACKGROUND	164:173	arg1	Obesity					175:181	BACKGROUND Obesity	164:181	BACKGROUND Obesity	164:181	BACKGROUND Obesity has reached global epidemic proportions and is associated with serious medical comorbidities and economic consequences.
30343042	9	26	theme	dopamine	1314:1321	arg1	D2					1323:1324	dopamine D2	1314:1324	the dopamine D2 receptor protein level	1310:1347	Finally, we report that only ad libitum high-sucrose evoked a significant enhancement of the dopamine D2 receptor protein level and a reduction in the D2 mRNA and receptor affinity in the rat striatum.
30343042	8	27	theme	diet	1038:1041	arg1	schedules					1043:1051	High-fat diet schedules	1029:1051	High-fat diet schedules	1029:1051	High-fat diet schedules resulted in the enhancement of leptin concentrations, while increases in blood levels of ghrelin were noted after intermitted high-fat or continuous high-sucrose diet.
30343042	10	28	theme	similar	1457:1463	arg1	reduction					1465:1473	a similar reduction	1455:1473	a similar reduction in dopamine D2 receptor affinity (decrease in KD value)	1455:1529	Independently of the diet type, a similar reduction in dopamine D2 receptor affinity (decrease in KD value) was found in the striatum of rats with intermittent food access.
30343042	9	29	theme	receptor	1326:1333	arg1	level					1343:1347	the dopamine D2 receptor protein level	1310:1347	the dopamine D2 receptor protein level	1310:1347	Finally, we report that only ad libitum high-sucrose evoked a significant enhancement of the dopamine D2 receptor protein level and a reduction in the D2 mRNA and receptor affinity in the rat striatum.
30343042	9	30	from	reduction	1355:1363	arg1	striatum					1413:1420	the rat striatum	1405:1420	the rat striatum	1405:1420	Finally, we report that only ad libitum high-sucrose evoked a significant enhancement of the dopamine D2 receptor protein level and a reduction in the D2 mRNA and receptor affinity in the rat striatum.
30343042	9	30	from	reduction	1355:1363	arg1	mRNA					1375:1378	the D2 mRNA	1368:1378	the D2 mRNA	1368:1378	Finally, we report that only ad libitum high-sucrose evoked a significant enhancement of the dopamine D2 receptor protein level and a reduction in the D2 mRNA and receptor affinity in the rat striatum.
30343042	10	31	dep	affinity	1499:1506	arg1	decrease					1509:1516	decrease	1509:1516	decrease in KD value	1509:1528	Independently of the diet type, a similar reduction in dopamine D2 receptor affinity (decrease in KD value) was found in the striatum of rats with intermittent food access.
30343042	4	32	theme	caloric	725:731	arg1	restriction					733:743	caloric restriction	725:743	caloric restriction	725:743	METHODS Male Wistar rats were fed with either high-fat or high-sucrose diet for 5 weeks according to different feeding regimes: ad libitum access or scheduled for a 2-h period each day without caloric restriction during the remainder of the day.
30343042	2	33	theme	caloric	399:405	arg1	intake					407:412	caloric intake	399:412	caloric intake	399:412	In this preclinical study, we characterized how the palatable diet changed food intake pattern, caloric intake, metabolic profile and hormone levels.
30343042	9	34	theme	level	1343:1347	arg1	affinity					1393:1400	receptor affinity	1384:1400	receptor affinity	1384:1400	Finally, we report that only ad libitum high-sucrose evoked a significant enhancement of the dopamine D2 receptor protein level and a reduction in the D2 mRNA and receptor affinity in the rat striatum.
30343042	9	34	theme	level	1343:1347	arg1	reduction					1355:1363	a reduction	1353:1363	a reduction in the D2 mRNA	1353:1378	Finally, we report that only ad libitum high-sucrose evoked a significant enhancement of the dopamine D2 receptor protein level and a reduction in the D2 mRNA and receptor affinity in the rat striatum.
30343042	9	34	theme	level	1343:1347	arg1	enhancement					1295:1305	a significant enhancement	1281:1305	a significant enhancement of the dopamine D2 receptor protein level	1281:1347	Finally, we report that only ad libitum high-sucrose evoked a significant enhancement of the dopamine D2 receptor protein level and a reduction in the D2 mRNA and receptor affinity in the rat striatum.
30343042	10	35	theme	D2	1487:1488	arg1	affinity					1499:1506	dopamine D2 receptor affinity	1478:1506	dopamine D2 receptor affinity (decrease in KD value)	1478:1529	Independently of the diet type, a similar reduction in dopamine D2 receptor affinity (decrease in KD value) was found in the striatum of rats with intermittent food access.
30343042	2	36	theme	hormone	437:443	arg1	levels					445:450	hormone levels	437:450	hormone levels	437:450	In this preclinical study, we characterized how the palatable diet changed food intake pattern, caloric intake, metabolic profile and hormone levels.
30343042	0	37	theme	metabolic	14:22	arg1	profiles					37:44	metabolic and hormonal profiles	14:44	metabolic and hormonal profiles	14:44	Assessment of metabolic and hormonal profiles and striatal dopamine D2 receptor expression following continuous or scheduled high-fat or high-sucrose diet in rats.
30343042	0	38	theme	hormonal	28:35	arg1	profiles					37:44	metabolic and hormonal profiles	14:44	metabolic and hormonal profiles	14:44	Assessment of metabolic and hormonal profiles and striatal dopamine D2 receptor expression following continuous or scheduled high-fat or high-sucrose diet in rats.
30343042	8	39	theme	concentrations	1091:1104	arg1	enhancement					1069:1079	the enhancement	1065:1079	the enhancement of leptin concentrations	1065:1104	High-fat diet schedules resulted in the enhancement of leptin concentrations, while increases in blood levels of ghrelin were noted after intermitted high-fat or continuous high-sucrose diet.
30343042	11	40	theme	disorders	1661:1669	arg1	understanding					1637:1649	a better understanding	1628:1649	a better understanding of eating disorders	1628:1669	CONCLUSION The findings provide a better understanding of eating disorders and indicate that diet composition leading to obesity induces distinct changes in dopamine D2 receptor signaling in the striatum.
30343042	3	41	theme	receptors	501:509	arg1	expression					475:484	the expression	471:484	the expression of dopamine D2 receptors in the rat striatum	471:529	We also evaluated the expression of dopamine D2 receptors in the rat striatum.
30343042	9	42	theme	D2	1372:1373	arg1	mRNA					1375:1378	the D2 mRNA	1368:1378	the D2 mRNA	1368:1378	Finally, we report that only ad libitum high-sucrose evoked a significant enhancement of the dopamine D2 receptor protein level and a reduction in the D2 mRNA and receptor affinity in the rat striatum.
30343042	4	43	theme	Wistar	545:550	arg1	rats					552:555	Male Wistar rats	540:555	Male Wistar rats	540:555	METHODS Male Wistar rats were fed with either high-fat or high-sucrose diet for 5 weeks according to different feeding regimes: ad libitum access or scheduled for a 2-h period each day without caloric restriction during the remainder of the day.
30343042	10	44	located	found	1535:1539	arg2	reduction					1465:1473	a similar reduction	1455:1473	a similar reduction in dopamine D2 receptor affinity (decrease in KD value)	1455:1529	Independently of the diet type, a similar reduction in dopamine D2 receptor affinity (decrease in KD value) was found in the striatum of rats with intermittent food access.
30343042	10	44	located	found	1535:1539	arg1	striatum					1548:1555	the striatum	1544:1555	the striatum of rats with intermittent food access	1544:1593	Independently of the diet type, a similar reduction in dopamine D2 receptor affinity (decrease in KD value) was found in the striatum of rats with intermittent food access.
30343042	3	45	theme	dopamine	489:496	arg1	receptors					501:509	dopamine D2 receptors	489:509	dopamine D2 receptors	489:509	We also evaluated the expression of dopamine D2 receptors in the rat striatum.
30343042	11	46	theme	diet	1689:1692	arg1	composition					1694:1704	diet composition	1689:1704	diet composition leading to obesity	1689:1723	CONCLUSION The findings provide a better understanding of eating disorders and indicate that diet composition leading to obesity induces distinct changes in dopamine D2 receptor signaling in the striatum.
30343042	1	47	theme	serious	246:252	arg1	comorbidities					262:274	serious medical comorbidities	246:274	serious medical comorbidities	246:274	BACKGROUND Obesity has reached global epidemic proportions and is associated with serious medical comorbidities and economic consequences.
30343042	10	48	theme	KD	1521:1522	arg1	value					1524:1528	KD value	1521:1528	KD value	1521:1528	Independently of the diet type, a similar reduction in dopamine D2 receptor affinity (decrease in KD value) was found in the striatum of rats with intermittent food access.
30343042	2	49	theme	food	378:381	arg1	intake					383:388	food intake pattern, caloric intake, metabolic profile and hormone levels	378:450	intake	383:388	In this preclinical study, we characterized how the palatable diet changed food intake pattern, caloric intake, metabolic profile and hormone levels.
30343042	8	50	theme	ghrelin	1142:1148	arg1	levels					1132:1137	blood levels	1126:1137	blood levels of ghrelin	1126:1148	High-fat diet schedules resulted in the enhancement of leptin concentrations, while increases in blood levels of ghrelin were noted after intermitted high-fat or continuous high-sucrose diet.
30343042	6	51	theme	high-fat	892:899	arg1	diet					901:904	high-fat diet	892:904	high-fat diet	892:904	Post-meal data showed that high-fat diet increased cholesterol, triglycerides and glucose concentrations.
30343042	4	52	theme	high-fat	578:585	arg1	diet					603:606	either high-fat or high-sucrose diet	571:606	either high-fat or high-sucrose diet for 5 weeks	571:618	METHODS Male Wistar rats were fed with either high-fat or high-sucrose diet for 5 weeks according to different feeding regimes: ad libitum access or scheduled for a 2-h period each day without caloric restriction during the remainder of the day.
30343042	2	53	theme	preclinical	311:321	arg1	study					323:327	this preclinical study	306:327	this preclinical study	306:327	In this preclinical study, we characterized how the palatable diet changed food intake pattern, caloric intake, metabolic profile and hormone levels.
30343042	1	54	theme	economic	280:287	arg1	consequences					289:300	economic consequences	280:300	economic consequences	280:300	BACKGROUND Obesity has reached global epidemic proportions and is associated with serious medical comorbidities and economic consequences.
30343042	4	55	theme	high-sucrose	590:601	arg1	diet					603:606	either high-fat or high-sucrose diet	571:606	either high-fat or high-sucrose diet for 5 weeks	571:618	METHODS Male Wistar rats were fed with either high-fat or high-sucrose diet for 5 weeks according to different feeding regimes: ad libitum access or scheduled for a 2-h period each day without caloric restriction during the remainder of the day.
30343042	8	56	theme	intermitted	1167:1177	arg1	diet					1215:1218	intermitted high-fat or continuous high-sucrose diet	1167:1218	intermitted high-fat or continuous high-sucrose diet	1167:1218	High-fat diet schedules resulted in the enhancement of leptin concentrations, while increases in blood levels of ghrelin were noted after intermitted high-fat or continuous high-sucrose diet.
30343042	6	57	theme	Post-meal	865:873	arg1	data					875:878	Post-meal data	865:878	Post-meal data	865:878	Post-meal data showed that high-fat diet increased cholesterol, triglycerides and glucose concentrations.
30343042	11	58	from	signaling	1774:1782	arg1	striatum					1791:1798	the striatum	1787:1798	the striatum	1787:1798	CONCLUSION The findings provide a better understanding of eating disorders and indicate that diet composition leading to obesity induces distinct changes in dopamine D2 receptor signaling in the striatum.
30343042	11	59	theme	better	1630:1635	arg1	understanding					1637:1649	a better understanding	1628:1649	a better understanding of eating disorders	1628:1669	CONCLUSION The findings provide a better understanding of eating disorders and indicate that diet composition leading to obesity induces distinct changes in dopamine D2 receptor signaling in the striatum.
30343042	5	60	theme	body	852:855	arg1	weight					857:862	total body weight	846:862	total body weight	846:862	RESULTS Both diets resulted in an enhancement in caloric intake and total body weight.
30343042	4	61	theme	libitum	663:669	arg1	access					671:676	ad libitum access	660:676	ad libitum access	660:676	METHODS Male Wistar rats were fed with either high-fat or high-sucrose diet for 5 weeks according to different feeding regimes: ad libitum access or scheduled for a 2-h period each day without caloric restriction during the remainder of the day.
30343042	1	62	theme	epidemic	202:209	arg1	proportions					211:221	global epidemic proportions	195:221	global epidemic proportions	195:221	BACKGROUND Obesity has reached global epidemic proportions and is associated with serious medical comorbidities and economic consequences.
30343042	8	63	theme	high-sucrose	1202:1213	arg1	diet					1215:1218	intermitted high-fat or continuous high-sucrose diet	1167:1218	intermitted high-fat or continuous high-sucrose diet	1167:1218	High-fat diet schedules resulted in the enhancement of leptin concentrations, while increases in blood levels of ghrelin were noted after intermitted high-fat or continuous high-sucrose diet.
30343042	5	64	theme	caloric	827:833	arg1	intake					835:840	caloric intake	827:840	caloric intake	827:840	RESULTS Both diets resulted in an enhancement in caloric intake and total body weight.
30343042	4	65	theme	feeding	643:649	arg1	regimes					651:657	different feeding regimes	633:657	different feeding regimes	633:657	METHODS Male Wistar rats were fed with either high-fat or high-sucrose diet for 5 weeks according to different feeding regimes: ad libitum access or scheduled for a 2-h period each day without caloric restriction during the remainder of the day.
30343042	10	66	theme	food	1583:1586	arg1	access					1588:1593	intermittent food access	1570:1593	intermittent food access	1570:1593	Independently of the diet type, a similar reduction in dopamine D2 receptor affinity (decrease in KD value) was found in the striatum of rats with intermittent food access.
30343042	9	67	from	enhancement	1295:1305	arg1	striatum					1413:1420	the rat striatum	1405:1420	the rat striatum	1405:1420	Finally, we report that only ad libitum high-sucrose evoked a significant enhancement of the dopamine D2 receptor protein level and a reduction in the D2 mRNA and receptor affinity in the rat striatum.
30343042	9	67	from	enhancement	1295:1305	arg1	mRNA					1375:1378	the D2 mRNA	1368:1378	the D2 mRNA	1368:1378	Finally, we report that only ad libitum high-sucrose evoked a significant enhancement of the dopamine D2 receptor protein level and a reduction in the D2 mRNA and receptor affinity in the rat striatum.
30343042	4	68	theme	2-h	697:699	arg1	period					701:706	a 2-h period	695:706	a 2-h period	695:706	METHODS Male Wistar rats were fed with either high-fat or high-sucrose diet for 5 weeks according to different feeding regimes: ad libitum access or scheduled for a 2-h period each day without caloric restriction during the remainder of the day.
30343042	11	69	theme	distinct	1733:1740	arg1	changes					1742:1748	distinct changes	1733:1748	distinct changes	1733:1748	CONCLUSION The findings provide a better understanding of eating disorders and indicate that diet composition leading to obesity induces distinct changes in dopamine D2 receptor signaling in the striatum.
30343042	0	70	theme	D2	68:69	arg1	expression					80:89	striatal dopamine D2 receptor expression	50:89	striatal dopamine D2 receptor expression	50:89	Assessment of metabolic and hormonal profiles and striatal dopamine D2 receptor expression following continuous or scheduled high-fat or high-sucrose diet in rats.
30343042	7	71	theme	sucrose	991:997	arg1	diet					999:1002	high sucrose diet	986:1002	high sucrose diet	986:1002	Animals fed on high sucrose diet were only hyperglycemic.
30343042	10	72	from	decrease	1509:1516	arg1	value					1524:1528	KD value	1521:1528	KD value	1521:1528	Independently of the diet type, a similar reduction in dopamine D2 receptor affinity (decrease in KD value) was found in the striatum of rats with intermittent food access.
30343042	0	73	theme	expression	80:89	arg1	Assessment					0:9	Assessment	0:9	Assessment of metabolic and hormonal profiles and striatal dopamine D2 receptor expression	0:89	Assessment of metabolic and hormonal profiles and striatal dopamine D2 receptor expression following continuous or scheduled high-fat or high-sucrose diet in rats.
30343042	8	74	theme	High-fat	1029:1036	arg1	schedules					1043:1051	High-fat diet schedules	1029:1051	High-fat diet schedules	1029:1051	High-fat diet schedules resulted in the enhancement of leptin concentrations, while increases in blood levels of ghrelin were noted after intermitted high-fat or continuous high-sucrose diet.
30343042	11	75	theme	D2	1762:1763	arg1	signaling					1774:1782	dopamine D2 receptor signaling	1753:1782	dopamine D2 receptor signaling in the striatum	1753:1798	CONCLUSION The findings provide a better understanding of eating disorders and indicate that diet composition leading to obesity induces distinct changes in dopamine D2 receptor signaling in the striatum.
30343042	0	76	theme	continuous	101:110	arg1	diet					150:153	continuous or scheduled high-fat or high-sucrose diet	101:153	continuous or scheduled high-fat or high-sucrose diet	101:153	Assessment of metabolic and hormonal profiles and striatal dopamine D2 receptor expression following continuous or scheduled high-fat or high-sucrose diet in rats.
30343042	4	77	dep	METHODS	532:538	arg1	fed					562:564	fed	562:564	fed with either high-fat or high-sucrose diet for 5 weeks according to different feeding regimes: ad libitum access	562:676	METHODS Male Wistar rats were fed with either high-fat or high-sucrose diet for 5 weeks according to different feeding regimes: ad libitum access or scheduled for a 2-h period each day without caloric restriction during the remainder of the day.
30343042	4	77	dep	METHODS	532:538	arg1	scheduled					681:689	scheduled	681:689	scheduled for a 2-h period each day without caloric restriction during the remainder of the day	681:775	METHODS Male Wistar rats were fed with either high-fat or high-sucrose diet for 5 weeks according to different feeding regimes: ad libitum access or scheduled for a 2-h period each day without caloric restriction during the remainder of the day.
30343042	8	78	from	increases	1113:1121	arg1	levels					1132:1137	blood levels	1126:1137	blood levels of ghrelin	1126:1148	High-fat diet schedules resulted in the enhancement of leptin concentrations, while increases in blood levels of ghrelin were noted after intermitted high-fat or continuous high-sucrose diet.
30343042	0	79	theme	scheduled	115:123	arg1	diet					150:153	continuous or scheduled high-fat or high-sucrose diet	101:153	continuous or scheduled high-fat or high-sucrose diet	101:153	Assessment of metabolic and hormonal profiles and striatal dopamine D2 receptor expression following continuous or scheduled high-fat or high-sucrose diet in rats.
30343042	9	80	theme	D2	1323:1324	arg1	level					1343:1347	the dopamine D2 receptor protein level	1310:1347	the dopamine D2 receptor protein level	1310:1347	Finally, we report that only ad libitum high-sucrose evoked a significant enhancement of the dopamine D2 receptor protein level and a reduction in the D2 mRNA and receptor affinity in the rat striatum.
30343042	2	81	dep	intake	383:388	arg1	pattern					390:396	pattern	390:396	pattern	390:396	In this preclinical study, we characterized how the palatable diet changed food intake pattern, caloric intake, metabolic profile and hormone levels.
30343042	9	82	theme	protein	1335:1341	arg1	level					1343:1347	the dopamine D2 receptor protein level	1310:1347	the dopamine D2 receptor protein level	1310:1347	Finally, we report that only ad libitum high-sucrose evoked a significant enhancement of the dopamine D2 receptor protein level and a reduction in the D2 mRNA and receptor affinity in the rat striatum.
30343042	4	83	dep	day	713:715	arg1	remainder					756:764	the remainder	752:764	the remainder of the day	752:775	METHODS Male Wistar rats were fed with either high-fat or high-sucrose diet for 5 weeks according to different feeding regimes: ad libitum access or scheduled for a 2-h period each day without caloric restriction during the remainder of the day.
30343042	8	84	theme	leptin	1084:1089	arg1	concentrations					1091:1104	leptin concentrations	1084:1104	leptin concentrations	1084:1104	High-fat diet schedules resulted in the enhancement of leptin concentrations, while increases in blood levels of ghrelin were noted after intermitted high-fat or continuous high-sucrose diet.
30343042	10	85	theme	dopamine	1478:1485	arg1	D2					1487:1488	dopamine D2	1478:1488	dopamine D2 receptor affinity (decrease in KD value)	1478:1529	Independently of the diet type, a similar reduction in dopamine D2 receptor affinity (decrease in KD value) was found in the striatum of rats with intermittent food access.
30343042	0	86	theme	profiles	37:44	arg1	Assessment					0:9	Assessment	0:9	Assessment of metabolic and hormonal profiles and striatal dopamine D2 receptor expression	0:89	Assessment of metabolic and hormonal profiles and striatal dopamine D2 receptor expression following continuous or scheduled high-fat or high-sucrose diet in rats.
30343042	4	87	dep	regimes	651:657	arg1	access					671:676	ad libitum access	660:676	ad libitum access	660:676	METHODS Male Wistar rats were fed with either high-fat or high-sucrose diet for 5 weeks according to different feeding regimes: ad libitum access or scheduled for a 2-h period each day without caloric restriction during the remainder of the day.
30343042	4	88	theme	Male	540:543	arg1	rats					552:555	Male Wistar rats	540:555	Male Wistar rats	540:555	METHODS Male Wistar rats were fed with either high-fat or high-sucrose diet for 5 weeks according to different feeding regimes: ad libitum access or scheduled for a 2-h period each day without caloric restriction during the remainder of the day.
30343042	11	89	theme	eating	1654:1659	arg1	disorders					1661:1669	eating disorders	1654:1669	eating disorders	1654:1669	CONCLUSION The findings provide a better understanding of eating disorders and indicate that diet composition leading to obesity induces distinct changes in dopamine D2 receptor signaling in the striatum.
30343042	2	90	theme	metabolic	415:423	arg1	profile					425:431	metabolic profile	415:431	metabolic profile	415:431	In this preclinical study, we characterized how the palatable diet changed food intake pattern, caloric intake, metabolic profile and hormone levels.
30343042	0	91	theme	striatal	50:57	arg1	dopamine					59:66	striatal dopamine	50:66	striatal dopamine D2 receptor expression	50:89	Assessment of metabolic and hormonal profiles and striatal dopamine D2 receptor expression following continuous or scheduled high-fat or high-sucrose diet in rats.
30343042	9	92	from	affinity	1393:1400	arg1	striatum					1413:1420	the rat striatum	1405:1420	the rat striatum	1405:1420	Finally, we report that only ad libitum high-sucrose evoked a significant enhancement of the dopamine D2 receptor protein level and a reduction in the D2 mRNA and receptor affinity in the rat striatum.
30343042	9	92	from	affinity	1393:1400	arg1	mRNA					1375:1378	the D2 mRNA	1368:1378	the D2 mRNA	1368:1378	Finally, we report that only ad libitum high-sucrose evoked a significant enhancement of the dopamine D2 receptor protein level and a reduction in the D2 mRNA and receptor affinity in the rat striatum.
30343042	3	93	from	expression	475:484	arg1	striatum					522:529	the rat striatum	514:529	the rat striatum	514:529	We also evaluated the expression of dopamine D2 receptors in the rat striatum.
30343042	2	94	theme	palatable	355:363	arg1	diet					365:368	the palatable diet	351:368	the palatable diet	351:368	In this preclinical study, we characterized how the palatable diet changed food intake pattern, caloric intake, metabolic profile and hormone levels.
30343042	8	95	theme	blood	1126:1130	arg1	levels					1132:1137	blood levels	1126:1137	blood levels of ghrelin	1126:1148	High-fat diet schedules resulted in the enhancement of leptin concentrations, while increases in blood levels of ghrelin were noted after intermitted high-fat or continuous high-sucrose diet.
30343042	10	96	with	rats	1560:1563	arg1	access					1588:1593	intermittent food access	1570:1593	intermittent food access	1570:1593	Independently of the diet type, a similar reduction in dopamine D2 receptor affinity (decrease in KD value) was found in the striatum of rats with intermittent food access.
30343042	10	97	theme	receptor	1490:1497	arg1	affinity					1499:1506	dopamine D2 receptor affinity	1478:1506	dopamine D2 receptor affinity (decrease in KD value)	1478:1529	Independently of the diet type, a similar reduction in dopamine D2 receptor affinity (decrease in KD value) was found in the striatum of rats with intermittent food access.
30343042	4	98	theme	day	773:775	arg1	remainder					756:764	the remainder	752:764	the remainder of the day	752:775	METHODS Male Wistar rats were fed with either high-fat or high-sucrose diet for 5 weeks according to different feeding regimes: ad libitum access or scheduled for a 2-h period each day without caloric restriction during the remainder of the day.
30343042	3	99	theme	D2	498:499	arg1	receptors					501:509	dopamine D2 receptors	489:509	dopamine D2 receptors	489:509	We also evaluated the expression of dopamine D2 receptors in the rat striatum.
30343042	9	100	theme	receptor	1384:1391	arg1	affinity					1393:1400	receptor affinity	1384:1400	receptor affinity	1384:1400	Finally, we report that only ad libitum high-sucrose evoked a significant enhancement of the dopamine D2 receptor protein level and a reduction in the D2 mRNA and receptor affinity in the rat striatum.
30343042	1	101	theme	medical	254:260	arg1	comorbidities					262:274	serious medical comorbidities	246:274	serious medical comorbidities	246:274	BACKGROUND Obesity has reached global epidemic proportions and is associated with serious medical comorbidities and economic consequences.
31767225	3	0	theme	several	560:566	arg1	hours					568:572	several hours	560:572	several hours up to overnight for complete removal of all N-linked glycans	560:633	The commonly-used in-solution deglycosylation method is relatively time-consuming and requires several hours up to overnight for complete removal of all N-linked glycans.
31767225	1	1	theme	key	253:255	arg1	process					240:246	An efficient deglycosylation process	211:246	An efficient deglycosylation process	211:246	An efficient deglycosylation process is a key requirement for the identification and characterization of glycosylation during the production and purification of therapeutic antibodies.
31767225	1	1	theme	key	253:255	arg1	requirement					257:267	a key requirement	251:267	a key requirement for the identification and characterization of glycosylation	251:328	An efficient deglycosylation process is a key requirement for the identification and characterization of glycosylation during the production and purification of therapeutic antibodies.
31767225	6	2	dep	pipette	1505:1511	arg1	referred					1524:1531	referred	1524:1531	referred to as manual pipetting times	1524:1560	The fixed enzymes maintained their activities in the polyacrylamide gel and the reaction was completed by 40 iterations of suction and discharge with a pipette (hereafter referred to as manual pipetting times) for 8 min with each enzyme digestion.
31767225	3	3	theme	complete	594:601	arg1	removal					603:609	complete removal	594:609	complete removal of all N-linked glycans	594:633	The commonly-used in-solution deglycosylation method is relatively time-consuming and requires several hours up to overnight for complete removal of all N-linked glycans.
31767225	6	4	theme	pipetting	1546:1554	arg1	times					1556:1560	manual pipetting times	1539:1560	manual pipetting times	1539:1560	The fixed enzymes maintained their activities in the polyacrylamide gel and the reaction was completed by 40 iterations of suction and discharge with a pipette (hereafter referred to as manual pipetting times) for 8 min with each enzyme digestion.
31767225	5	5	theme	solution	1231:1238	arg1	polymerization					1209:1222	situ polymerization	1204:1222	situ polymerization of gel solution approximately 30 mm from the tip	1204:1271	Our enzyme reactor is based on simple photochemical copolymerization of monomers using the following procedure: (1) a pipette tip was filled with a gel solution comprising acrylamide, N,N'-methylene-bis-acrylamide containing PNGase F or trypsin with 2,2-azobis(2-methyl-N-(2-hydroxyethyl) propionamide) as a photocatalytic initiator; and (2) in situ polymerization of gel solution approximately 30 mm from the tip was performed by irradiation with a 365 nm blue LED beam from a distance 10 mm.
31767225	5	6	from	tip	1269:1271	arg1	30 mm					1254:1258	30 mm	1254:1258	30 mm	1254:1258	Our enzyme reactor is based on simple photochemical copolymerization of monomers using the following procedure: (1) a pipette tip was filled with a gel solution comprising acrylamide, N,N'-methylene-bis-acrylamide containing PNGase F or trypsin with 2,2-azobis(2-methyl-N-(2-hydroxyethyl) propionamide) as a photocatalytic initiator; and (2) in situ polymerization of gel solution approximately 30 mm from the tip was performed by irradiation with a 365 nm blue LED beam from a distance 10 mm.
31767225	4	7	theme	rapid	694:698	arg1	release					700:706	the rapid release	690:706	the rapid release of N-linked glycans from glycoproteins	690:745	In order to develop a simple and efficient method for the rapid release of N-linked glycans from glycoproteins, we fabricated trypsin- and PNGase F-impregnated polyacrylamide gels in a commercial 200 μL volume pipette tip.
31767225	0	8	theme	pipette	198:204	arg1	tip					206:208	a pipette tip	196:208	a pipette tip	196:208	A fast and convenient solid phase preparation method for releasing N-glycans from glycoproteins using trypsin- and peptide-N-glycosidase F (PNGase F)-impregnated polyacrylamide gels fabricated in a pipette tip.
31767225	3	9	theme	overnight	580:588	arg1	hours					568:572	several hours	560:572	several hours up to overnight for complete removal of all N-linked glycans	560:633	The commonly-used in-solution deglycosylation method is relatively time-consuming and requires several hours up to overnight for complete removal of all N-linked glycans.
31767225	3	10	theme	N-linked	618:625	arg1	glycans					627:633	all N-linked glycans	614:633	all N-linked glycans	614:633	The commonly-used in-solution deglycosylation method is relatively time-consuming and requires several hours up to overnight for complete removal of all N-linked glycans.
31767225	5	11	theme	monomers	931:938	arg1	copolymerization					911:926	simple photochemical copolymerization	890:926	simple photochemical copolymerization of monomers using the following procedure	890:968	Our enzyme reactor is based on simple photochemical copolymerization of monomers using the following procedure: (1) a pipette tip was filled with a gel solution comprising acrylamide, N,N'-methylene-bis-acrylamide containing PNGase F or trypsin with 2,2-azobis(2-methyl-N-(2-hydroxyethyl) propionamide) as a photocatalytic initiator; and (2) in situ polymerization of gel solution approximately 30 mm from the tip was performed by irradiation with a 365 nm blue LED beam from a distance 10 mm.
31767225	7	12	theme	released	1635:1642	arg1	glycans					1644:1650	released glycans	1635:1650	released glycans labeled with 8-aminopyrene-1,3,6-trisulfonate (APTS)	1635:1703	Capillary electrophoresis (CE) of released glycans labeled with 8-aminopyrene-1,3,6-trisulfonate (APTS) demonstrated quantitative recovery of glycans from selected glycoproteins.
31767225	7	13	from	glycoproteins	1765:1777	arg1	recovery					1731:1738	quantitative recovery	1718:1738	quantitative recovery of glycans from selected glycoproteins	1718:1777	Capillary electrophoresis (CE) of released glycans labeled with 8-aminopyrene-1,3,6-trisulfonate (APTS) demonstrated quantitative recovery of glycans from selected glycoproteins.
31767225	4	14	theme	efficient	669:677	arg1	method					679:684	a simple and efficient method	656:684	a simple and efficient method for the rapid release of N-linked glycans from glycoproteins	656:745	In order to develop a simple and efficient method for the rapid release of N-linked glycans from glycoproteins, we fabricated trypsin- and PNGase F-impregnated polyacrylamide gels in a commercial 200 μL volume pipette tip.
31767225	0	15	theme	peptide-N-glycosidase	115:135	arg1	F					137:137	peptide-N-glycosidase F	115:137	peptide-N-glycosidase F (PNGase F)	115:148	A fast and convenient solid phase preparation method for releasing N-glycans from glycoproteins using trypsin- and peptide-N-glycosidase F (PNGase F)-impregnated polyacrylamide gels fabricated in a pipette tip.
31767225	0	15	theme	peptide-N-glycosidase	115:135	arg1	F					147:147	PNGase F	140:147	PNGase F	140:147	A fast and convenient solid phase preparation method for releasing N-glycans from glycoproteins using trypsin- and peptide-N-glycosidase F (PNGase F)-impregnated polyacrylamide gels fabricated in a pipette tip.
31767225	5	16	theme	enzyme	863:868	arg1	reactor					870:876	Our enzyme reactor	859:876	Our enzyme reactor	859:876	Our enzyme reactor is based on simple photochemical copolymerization of monomers using the following procedure: (1) a pipette tip was filled with a gel solution comprising acrylamide, N,N'-methylene-bis-acrylamide containing PNGase F or trypsin with 2,2-azobis(2-methyl-N-(2-hydroxyethyl) propionamide) as a photocatalytic initiator; and (2) in situ polymerization of gel solution approximately 30 mm from the tip was performed by irradiation with a 365 nm blue LED beam from a distance 10 mm.
31767225	5	17	theme	simple	890:895	arg1	copolymerization					911:926	simple photochemical copolymerization	890:926	simple photochemical copolymerization of monomers using the following procedure	890:968	Our enzyme reactor is based on simple photochemical copolymerization of monomers using the following procedure: (1) a pipette tip was filled with a gel solution comprising acrylamide, N,N'-methylene-bis-acrylamide containing PNGase F or trypsin with 2,2-azobis(2-methyl-N-(2-hydroxyethyl) propionamide) as a photocatalytic initiator; and (2) in situ polymerization of gel solution approximately 30 mm from the tip was performed by irradiation with a 365 nm blue LED beam from a distance 10 mm.
31767225	6	18	with	pipette	1505:1511	arg1	digestion					1590:1598	each enzyme digestion	1578:1598	each enzyme digestion	1578:1598	The fixed enzymes maintained their activities in the polyacrylamide gel and the reaction was completed by 40 iterations of suction and discharge with a pipette (hereafter referred to as manual pipetting times) for 8 min with each enzyme digestion.
31767225	5	19	theme	PNGase	1084:1089	arg1	F					1091:1091	PNGase F	1084:1091	PNGase F	1084:1091	Our enzyme reactor is based on simple photochemical copolymerization of monomers using the following procedure: (1) a pipette tip was filled with a gel solution comprising acrylamide, N,N'-methylene-bis-acrylamide containing PNGase F or trypsin with 2,2-azobis(2-methyl-N-(2-hydroxyethyl) propionamide) as a photocatalytic initiator; and (2) in situ polymerization of gel solution approximately 30 mm from the tip was performed by irradiation with a 365 nm blue LED beam from a distance 10 mm.
31767225	3	20	dep	overnight	580:588	arg1	to					577:578	to	577:578	to	577:578	The commonly-used in-solution deglycosylation method is relatively time-consuming and requires several hours up to overnight for complete removal of all N-linked glycans.
31767225	7	21	theme	Capillary	1601:1609	arg1	electrophoresis					1611:1625	Capillary electrophoresis	1601:1625	Capillary electrophoresis (CE) of released glycans labeled with 8-aminopyrene-1,3,6-trisulfonate (APTS)	1601:1703	Capillary electrophoresis (CE) of released glycans labeled with 8-aminopyrene-1,3,6-trisulfonate (APTS) demonstrated quantitative recovery of glycans from selected glycoproteins.
31767225	7	21	theme	Capillary	1601:1609	arg1	CE					1628:1629	CE	1628:1629	CE	1628:1629	Capillary electrophoresis (CE) of released glycans labeled with 8-aminopyrene-1,3,6-trisulfonate (APTS) demonstrated quantitative recovery of glycans from selected glycoproteins.
31767225	5	22	from	10 mm	1346:1350	arg1	beam					1325:1328	beam	1325:1328	beam from a distance 10 mm	1325:1350	Our enzyme reactor is based on simple photochemical copolymerization of monomers using the following procedure: (1) a pipette tip was filled with a gel solution comprising acrylamide, N,N'-methylene-bis-acrylamide containing PNGase F or trypsin with 2,2-azobis(2-methyl-N-(2-hydroxyethyl) propionamide) as a photocatalytic initiator; and (2) in situ polymerization of gel solution approximately 30 mm from the tip was performed by irradiation with a 365 nm blue LED beam from a distance 10 mm.
31767225	5	23	dep	filled	993:998	arg1	1					972:972	1	972:972	1	972:972	Our enzyme reactor is based on simple photochemical copolymerization of monomers using the following procedure: (1) a pipette tip was filled with a gel solution comprising acrylamide, N,N'-methylene-bis-acrylamide containing PNGase F or trypsin with 2,2-azobis(2-methyl-N-(2-hydroxyethyl) propionamide) as a photocatalytic initiator; and (2) in situ polymerization of gel solution approximately 30 mm from the tip was performed by irradiation with a 365 nm blue LED beam from a distance 10 mm.
31767225	4	24	gly	glycoproteins	733:745	arg1	glycoproteins					733:745	glycoproteins	733:745	glycoproteins	733:745	In order to develop a simple and efficient method for the rapid release of N-linked glycans from glycoproteins, we fabricated trypsin- and PNGase F-impregnated polyacrylamide gels in a commercial 200 μL volume pipette tip.
31767225	4	25	theme	trypsin-	762:769	arg1	gels					811:814	trypsin- and PNGase F-impregnated polyacrylamide gels	762:814	trypsin- and PNGase F-impregnated polyacrylamide gels	762:814	In order to develop a simple and efficient method for the rapid release of N-linked glycans from glycoproteins, we fabricated trypsin- and PNGase F-impregnated polyacrylamide gels in a commercial 200 μL volume pipette tip.
31767225	0	26	from	glycoproteins	82:94	arg1	N-glycans					67:75	N-glycans	67:75	N-glycans from glycoproteins using trypsin- and peptide-N-glycosidase F (PNGase F)-impregnated polyacrylamide gels fabricated in a pipette tip	67:208	A fast and convenient solid phase preparation method for releasing N-glycans from glycoproteins using trypsin- and peptide-N-glycosidase F (PNGase F)-impregnated polyacrylamide gels fabricated in a pipette tip.
31767225	4	27	theme	glycans	720:726	arg1	release					700:706	the rapid release	690:706	the rapid release of N-linked glycans from glycoproteins	690:745	In order to develop a simple and efficient method for the rapid release of N-linked glycans from glycoproteins, we fabricated trypsin- and PNGase F-impregnated polyacrylamide gels in a commercial 200 μL volume pipette tip.
31767225	2	28	gly	deglycosylation	428:442	arg1	glycans					456:462	N-linked glycans	447:462	N-linked glycans	447:462	PNGase F is widely used for the deglycosylation of N-linked glycans.
31767225	2	29	used	used	415:418	arg2	F					403:403	PNGase F	396:403	PNGase F	396:403	PNGase F is widely used for the deglycosylation of N-linked glycans.
31767225	7	30	theme	quantitative	1718:1729	arg1	recovery					1731:1738	quantitative recovery	1718:1738	quantitative recovery of glycans from selected glycoproteins	1718:1777	Capillary electrophoresis (CE) of released glycans labeled with 8-aminopyrene-1,3,6-trisulfonate (APTS) demonstrated quantitative recovery of glycans from selected glycoproteins.
31767225	1	31	dep	identification	277:290	arg1	the					273:275	the	273:275	the	273:275	An efficient deglycosylation process is a key requirement for the identification and characterization of glycosylation during the production and purification of therapeutic antibodies.
31767225	2	32	link	N-linked	447:454	arg1	glycans					456:462	N-linked glycans	447:462	N-linked glycans	447:462	PNGase F is widely used for the deglycosylation of N-linked glycans.
31767225	1	33	theme	therapeutic	372:382	arg1	antibodies					384:393	therapeutic antibodies	372:393	therapeutic antibodies	372:393	An efficient deglycosylation process is a key requirement for the identification and characterization of glycosylation during the production and purification of therapeutic antibodies.
31767225	4	34	theme	commercial	821:830	arg1	tip					854:856	a commercial 200 μL volume pipette tip	819:856	a commercial 200 μL volume pipette tip	819:856	In order to develop a simple and efficient method for the rapid release of N-linked glycans from glycoproteins, we fabricated trypsin- and PNGase F-impregnated polyacrylamide gels in a commercial 200 μL volume pipette tip.
31767225	5	35	theme	distance	1337:1344	arg1	10 mm					1346:1350	a distance 10 mm	1335:1350	a distance 10 mm	1335:1350	Our enzyme reactor is based on simple photochemical copolymerization of monomers using the following procedure: (1) a pipette tip was filled with a gel solution comprising acrylamide, N,N'-methylene-bis-acrylamide containing PNGase F or trypsin with 2,2-azobis(2-methyl-N-(2-hydroxyethyl) propionamide) as a photocatalytic initiator; and (2) in situ polymerization of gel solution approximately 30 mm from the tip was performed by irradiation with a 365 nm blue LED beam from a distance 10 mm.
31767225	0	36	theme	trypsin-	102:109	arg1	gels					177:180	trypsin- and peptide-N-glycosidase F (PNGase F)-impregnated polyacrylamide gels	102:180	trypsin- and peptide-N-glycosidase F (PNGase F)-impregnated polyacrylamide gels fabricated in a pipette tip	102:208	A fast and convenient solid phase preparation method for releasing N-glycans from glycoproteins using trypsin- and peptide-N-glycosidase F (PNGase F)-impregnated polyacrylamide gels fabricated in a pipette tip.
31767225	5	37	with	irradiation	1290:1300	arg1	365 nm					1309:1314	a 365 nm blue	1307:1319	a 365 nm blue LED beam from a distance 10 mm	1307:1350	Our enzyme reactor is based on simple photochemical copolymerization of monomers using the following procedure: (1) a pipette tip was filled with a gel solution comprising acrylamide, N,N'-methylene-bis-acrylamide containing PNGase F or trypsin with 2,2-azobis(2-methyl-N-(2-hydroxyethyl) propionamide) as a photocatalytic initiator; and (2) in situ polymerization of gel solution approximately 30 mm from the tip was performed by irradiation with a 365 nm blue LED beam from a distance 10 mm.
31767225	5	38	theme	gel	1007:1009	arg1	solution					1011:1018	a gel solution	1005:1018	a gel solution comprising acrylamide, N,N'-methylene-bis-acrylamide containing PNGase F or trypsin with 2,2-azobis(2-methyl-N-(2-hydroxyethyl) propionamide) as a photocatalytic initiator	1005:1190	Our enzyme reactor is based on simple photochemical copolymerization of monomers using the following procedure: (1) a pipette tip was filled with a gel solution comprising acrylamide, N,N'-methylene-bis-acrylamide containing PNGase F or trypsin with 2,2-azobis(2-methyl-N-(2-hydroxyethyl) propionamide) as a photocatalytic initiator; and (2) in situ polymerization of gel solution approximately 30 mm from the tip was performed by irradiation with a 365 nm blue LED beam from a distance 10 mm.
31767225	4	39	theme	PNGase	775:780	arg1	gels					811:814	trypsin- and PNGase F-impregnated polyacrylamide gels	762:814	trypsin- and PNGase F-impregnated polyacrylamide gels	762:814	In order to develop a simple and efficient method for the rapid release of N-linked glycans from glycoproteins, we fabricated trypsin- and PNGase F-impregnated polyacrylamide gels in a commercial 200 μL volume pipette tip.
31767225	5	40	theme	pipette	977:983	arg1	tip					985:987	a pipette tip	975:987	a pipette tip	975:987	Our enzyme reactor is based on simple photochemical copolymerization of monomers using the following procedure: (1) a pipette tip was filled with a gel solution comprising acrylamide, N,N'-methylene-bis-acrylamide containing PNGase F or trypsin with 2,2-azobis(2-methyl-N-(2-hydroxyethyl) propionamide) as a photocatalytic initiator; and (2) in situ polymerization of gel solution approximately 30 mm from the tip was performed by irradiation with a 365 nm blue LED beam from a distance 10 mm.
31767225	2	41	theme	glycans	456:462	arg1	deglycosylation					428:442	the deglycosylation	424:442	the deglycosylation of N-linked glycans	424:462	PNGase F is widely used for the deglycosylation of N-linked glycans.
31767225	4	42	theme	polyacrylamide	796:809	arg1	gels					811:814	trypsin- and PNGase F-impregnated polyacrylamide gels	762:814	trypsin- and PNGase F-impregnated polyacrylamide gels	762:814	In order to develop a simple and efficient method for the rapid release of N-linked glycans from glycoproteins, we fabricated trypsin- and PNGase F-impregnated polyacrylamide gels in a commercial 200 μL volume pipette tip.
31767225	3	43	theme	deglycosylation	495:509	arg1	time-consuming					532:545	time-consuming	532:545	time-consuming	532:545	The commonly-used in-solution deglycosylation method is relatively time-consuming and requires several hours up to overnight for complete removal of all N-linked glycans.
31767225	3	43	theme	deglycosylation	495:509	arg1	method					511:516	The commonly-used in-solution deglycosylation method	465:516	The commonly-used in-solution deglycosylation method	465:516	The commonly-used in-solution deglycosylation method is relatively time-consuming and requires several hours up to overnight for complete removal of all N-linked glycans.
31767225	7	44	theme	glycans	1644:1650	arg1	electrophoresis					1611:1625	Capillary electrophoresis	1601:1625	Capillary electrophoresis (CE) of released glycans labeled with 8-aminopyrene-1,3,6-trisulfonate (APTS)	1601:1703	Capillary electrophoresis (CE) of released glycans labeled with 8-aminopyrene-1,3,6-trisulfonate (APTS) demonstrated quantitative recovery of glycans from selected glycoproteins.
31767225	7	44	theme	glycans	1644:1650	arg1	CE					1628:1629	CE	1628:1629	CE	1628:1629	Capillary electrophoresis (CE) of released glycans labeled with 8-aminopyrene-1,3,6-trisulfonate (APTS) demonstrated quantitative recovery of glycans from selected glycoproteins.
31767225	3	45	theme	commonly-used	469:481	arg1	time-consuming					532:545	time-consuming	532:545	time-consuming	532:545	The commonly-used in-solution deglycosylation method is relatively time-consuming and requires several hours up to overnight for complete removal of all N-linked glycans.
31767225	3	45	theme	commonly-used	469:481	arg1	method					511:516	The commonly-used in-solution deglycosylation method	465:516	The commonly-used in-solution deglycosylation method	465:516	The commonly-used in-solution deglycosylation method is relatively time-consuming and requires several hours up to overnight for complete removal of all N-linked glycans.
31767225	0	46	theme	solid	22:26	arg1	method					46:51	A fast and convenient solid phase preparation method	0:51	A fast and convenient solid phase preparation method for releasing N-glycans from glycoproteins using trypsin- and peptide-N-glycosidase F (PNGase F)-impregnated polyacrylamide gels fabricated in a pipette tip.	0:209	A fast and convenient solid phase preparation method for releasing N-glycans from glycoproteins using trypsin- and peptide-N-glycosidase F (PNGase F)-impregnated polyacrylamide gels fabricated in a pipette tip.
31767225	0	47	theme	preparation	34:44	arg1	method					46:51	A fast and convenient solid phase preparation method	0:51	A fast and convenient solid phase preparation method for releasing N-glycans from glycoproteins using trypsin- and peptide-N-glycosidase F (PNGase F)-impregnated polyacrylamide gels fabricated in a pipette tip.	0:209	A fast and convenient solid phase preparation method for releasing N-glycans from glycoproteins using trypsin- and peptide-N-glycosidase F (PNGase F)-impregnated polyacrylamide gels fabricated in a pipette tip.
31767225	5	48	contain	containing	1073:1082	arg1	acrylamide					1031:1040	acrylamide	1031:1040	acrylamide	1031:1040	Our enzyme reactor is based on simple photochemical copolymerization of monomers using the following procedure: (1) a pipette tip was filled with a gel solution comprising acrylamide, N,N'-methylene-bis-acrylamide containing PNGase F or trypsin with 2,2-azobis(2-methyl-N-(2-hydroxyethyl) propionamide) as a photocatalytic initiator; and (2) in situ polymerization of gel solution approximately 30 mm from the tip was performed by irradiation with a 365 nm blue LED beam from a distance 10 mm.
31767225	5	48	contain	containing	1073:1082	arg1	N					1043:1043	N	1043:1043	N	1043:1043	Our enzyme reactor is based on simple photochemical copolymerization of monomers using the following procedure: (1) a pipette tip was filled with a gel solution comprising acrylamide, N,N'-methylene-bis-acrylamide containing PNGase F or trypsin with 2,2-azobis(2-methyl-N-(2-hydroxyethyl) propionamide) as a photocatalytic initiator; and (2) in situ polymerization of gel solution approximately 30 mm from the tip was performed by irradiation with a 365 nm blue LED beam from a distance 10 mm.
31767225	5	48	contain	containing	1073:1082	arg1	initiator					1182:1190	a photocatalytic initiator	1165:1190	a photocatalytic initiator	1165:1190	Our enzyme reactor is based on simple photochemical copolymerization of monomers using the following procedure: (1) a pipette tip was filled with a gel solution comprising acrylamide, N,N'-methylene-bis-acrylamide containing PNGase F or trypsin with 2,2-azobis(2-methyl-N-(2-hydroxyethyl) propionamide) as a photocatalytic initiator; and (2) in situ polymerization of gel solution approximately 30 mm from the tip was performed by irradiation with a 365 nm blue LED beam from a distance 10 mm.
31767225	5	48	contain	containing	1073:1082	arg1	N'-methylene-bis-acrylamide					1045:1071	N'-methylene-bis-acrylamide	1045:1071	N'-methylene-bis-acrylamide	1045:1071	Our enzyme reactor is based on simple photochemical copolymerization of monomers using the following procedure: (1) a pipette tip was filled with a gel solution comprising acrylamide, N,N'-methylene-bis-acrylamide containing PNGase F or trypsin with 2,2-azobis(2-methyl-N-(2-hydroxyethyl) propionamide) as a photocatalytic initiator; and (2) in situ polymerization of gel solution approximately 30 mm from the tip was performed by irradiation with a 365 nm blue LED beam from a distance 10 mm.
31767225	5	48	contain	containing	1073:1082	arg2	F					1091:1091	PNGase F	1084:1091	PNGase F	1084:1091	Our enzyme reactor is based on simple photochemical copolymerization of monomers using the following procedure: (1) a pipette tip was filled with a gel solution comprising acrylamide, N,N'-methylene-bis-acrylamide containing PNGase F or trypsin with 2,2-azobis(2-methyl-N-(2-hydroxyethyl) propionamide) as a photocatalytic initiator; and (2) in situ polymerization of gel solution approximately 30 mm from the tip was performed by irradiation with a 365 nm blue LED beam from a distance 10 mm.
31767225	5	48	contain	containing	1073:1082	arg2	trypsin					1096:1102	trypsin	1096:1102	trypsin	1096:1102	Our enzyme reactor is based on simple photochemical copolymerization of monomers using the following procedure: (1) a pipette tip was filled with a gel solution comprising acrylamide, N,N'-methylene-bis-acrylamide containing PNGase F or trypsin with 2,2-azobis(2-methyl-N-(2-hydroxyethyl) propionamide) as a photocatalytic initiator; and (2) in situ polymerization of gel solution approximately 30 mm from the tip was performed by irradiation with a 365 nm blue LED beam from a distance 10 mm.
31767225	4	49	theme	volume	839:844	arg1	tip					854:856	a commercial 200 μL volume pipette tip	819:856	a commercial 200 μL volume pipette tip	819:856	In order to develop a simple and efficient method for the rapid release of N-linked glycans from glycoproteins, we fabricated trypsin- and PNGase F-impregnated polyacrylamide gels in a commercial 200 μL volume pipette tip.
31767225	1	50	theme	efficient	214:222	arg1	process					240:246	An efficient deglycosylation process	211:246	An efficient deglycosylation process	211:246	An efficient deglycosylation process is a key requirement for the identification and characterization of glycosylation during the production and purification of therapeutic antibodies.
31767225	1	50	theme	efficient	214:222	arg1	requirement					257:267	a key requirement	251:267	a key requirement for the identification and characterization of glycosylation	251:328	An efficient deglycosylation process is a key requirement for the identification and characterization of glycosylation during the production and purification of therapeutic antibodies.
31767225	2	51	theme	PNGase	396:401	arg1	F					403:403	PNGase F	396:403	PNGase F	396:403	PNGase F is widely used for the deglycosylation of N-linked glycans.
31767225	5	52	theme	gel	1227:1229	arg1	solution					1231:1238	gel solution	1227:1238	gel solution approximately 30 mm from the tip	1227:1271	Our enzyme reactor is based on simple photochemical copolymerization of monomers using the following procedure: (1) a pipette tip was filled with a gel solution comprising acrylamide, N,N'-methylene-bis-acrylamide containing PNGase F or trypsin with 2,2-azobis(2-methyl-N-(2-hydroxyethyl) propionamide) as a photocatalytic initiator; and (2) in situ polymerization of gel solution approximately 30 mm from the tip was performed by irradiation with a 365 nm blue LED beam from a distance 10 mm.
31767225	6	53	dep	referred	1524:1531	arg1	hereafter					1514:1522	hereafter	1514:1522	hereafter	1514:1522	The fixed enzymes maintained their activities in the polyacrylamide gel and the reaction was completed by 40 iterations of suction and discharge with a pipette (hereafter referred to as manual pipetting times) for 8 min with each enzyme digestion.
31767225	6	54	with	iterations	1462:1471	arg1	pipette					1505:1511	a pipette	1503:1511	a pipette (hereafter referred to as manual pipetting times) for 8 min with each enzyme digestion	1503:1598	The fixed enzymes maintained their activities in the polyacrylamide gel and the reaction was completed by 40 iterations of suction and discharge with a pipette (hereafter referred to as manual pipetting times) for 8 min with each enzyme digestion.
31767225	6	55	theme	manual	1539:1544	arg1	times					1556:1560	manual pipetting times	1539:1560	manual pipetting times	1539:1560	The fixed enzymes maintained their activities in the polyacrylamide gel and the reaction was completed by 40 iterations of suction and discharge with a pipette (hereafter referred to as manual pipetting times) for 8 min with each enzyme digestion.
31767225	5	56	theme	photochemical	897:909	arg1	copolymerization					911:926	simple photochemical copolymerization	890:926	simple photochemical copolymerization of monomers using the following procedure	890:968	Our enzyme reactor is based on simple photochemical copolymerization of monomers using the following procedure: (1) a pipette tip was filled with a gel solution comprising acrylamide, N,N'-methylene-bis-acrylamide containing PNGase F or trypsin with 2,2-azobis(2-methyl-N-(2-hydroxyethyl) propionamide) as a photocatalytic initiator; and (2) in situ polymerization of gel solution approximately 30 mm from the tip was performed by irradiation with a 365 nm blue LED beam from a distance 10 mm.
31767225	6	57	theme	polyacrylamide	1406:1419	arg1	gel					1421:1423	the polyacrylamide gel	1402:1423	the polyacrylamide gel	1402:1423	The fixed enzymes maintained their activities in the polyacrylamide gel and the reaction was completed by 40 iterations of suction and discharge with a pipette (hereafter referred to as manual pipetting times) for 8 min with each enzyme digestion.
31767225	4	58	link	N-linked	711:718	arg1	glycans					720:726	N-linked glycans	711:726	N-linked glycans	711:726	In order to develop a simple and efficient method for the rapid release of N-linked glycans from glycoproteins, we fabricated trypsin- and PNGase F-impregnated polyacrylamide gels in a commercial 200 μL volume pipette tip.
31767225	0	59	theme	PNGase	140:145	arg1	F					137:137	peptide-N-glycosidase F	115:137	peptide-N-glycosidase F (PNGase F)	115:148	A fast and convenient solid phase preparation method for releasing N-glycans from glycoproteins using trypsin- and peptide-N-glycosidase F (PNGase F)-impregnated polyacrylamide gels fabricated in a pipette tip.
31767225	0	59	theme	PNGase	140:145	arg1	F					147:147	PNGase F	140:147	PNGase F	140:147	A fast and convenient solid phase preparation method for releasing N-glycans from glycoproteins using trypsin- and peptide-N-glycosidase F (PNGase F)-impregnated polyacrylamide gels fabricated in a pipette tip.
31767225	5	60	theme	2-methyl-N-	1120:1130	arg1	2,2-azobis					1109:1118	2,2-azobis	1109:1118	2,2-azobis(2-methyl-N-(2-hydroxyethyl) propionamide)	1109:1160	Our enzyme reactor is based on simple photochemical copolymerization of monomers using the following procedure: (1) a pipette tip was filled with a gel solution comprising acrylamide, N,N'-methylene-bis-acrylamide containing PNGase F or trypsin with 2,2-azobis(2-methyl-N-(2-hydroxyethyl) propionamide) as a photocatalytic initiator; and (2) in situ polymerization of gel solution approximately 30 mm from the tip was performed by irradiation with a 365 nm blue LED beam from a distance 10 mm.
31767225	5	60	theme	2-methyl-N-	1120:1130	arg1	propionamide					1148:1159	2-methyl-N-(2-hydroxyethyl) propionamide	1120:1159	2-methyl-N-(2-hydroxyethyl) propionamide	1120:1159	Our enzyme reactor is based on simple photochemical copolymerization of monomers using the following procedure: (1) a pipette tip was filled with a gel solution comprising acrylamide, N,N'-methylene-bis-acrylamide containing PNGase F or trypsin with 2,2-azobis(2-methyl-N-(2-hydroxyethyl) propionamide) as a photocatalytic initiator; and (2) in situ polymerization of gel solution approximately 30 mm from the tip was performed by irradiation with a 365 nm blue LED beam from a distance 10 mm.
31767225	3	61	theme	glycans	627:633	arg1	removal					603:609	complete removal	594:609	complete removal of all N-linked glycans	594:633	The commonly-used in-solution deglycosylation method is relatively time-consuming and requires several hours up to overnight for complete removal of all N-linked glycans.
31767225	6	62	theme	enzyme	1583:1588	arg1	digestion					1590:1598	each enzyme digestion	1578:1598	each enzyme digestion	1578:1598	The fixed enzymes maintained their activities in the polyacrylamide gel and the reaction was completed by 40 iterations of suction and discharge with a pipette (hereafter referred to as manual pipetting times) for 8 min with each enzyme digestion.
31767225	0	63	theme	polyacrylamide	162:175	arg1	gels					177:180	trypsin- and peptide-N-glycosidase F (PNGase F)-impregnated polyacrylamide gels	102:180	trypsin- and peptide-N-glycosidase F (PNGase F)-impregnated polyacrylamide gels fabricated in a pipette tip	102:208	A fast and convenient solid phase preparation method for releasing N-glycans from glycoproteins using trypsin- and peptide-N-glycosidase F (PNGase F)-impregnated polyacrylamide gels fabricated in a pipette tip.
31767225	7	64	theme	selected	1756:1763	arg1	glycoproteins					1765:1777	selected glycoproteins	1756:1777	selected glycoproteins	1756:1777	Capillary electrophoresis (CE) of released glycans labeled with 8-aminopyrene-1,3,6-trisulfonate (APTS) demonstrated quantitative recovery of glycans from selected glycoproteins.
31767225	4	65	theme	N-linked	711:718	arg1	glycans					720:726	N-linked glycans	711:726	N-linked glycans	711:726	In order to develop a simple and efficient method for the rapid release of N-linked glycans from glycoproteins, we fabricated trypsin- and PNGase F-impregnated polyacrylamide gels in a commercial 200 μL volume pipette tip.
31767225	5	66	theme	following	950:958	arg1	procedure					960:968	the following procedure	946:968	the following procedure	946:968	Our enzyme reactor is based on simple photochemical copolymerization of monomers using the following procedure: (1) a pipette tip was filled with a gel solution comprising acrylamide, N,N'-methylene-bis-acrylamide containing PNGase F or trypsin with 2,2-azobis(2-methyl-N-(2-hydroxyethyl) propionamide) as a photocatalytic initiator; and (2) in situ polymerization of gel solution approximately 30 mm from the tip was performed by irradiation with a 365 nm blue LED beam from a distance 10 mm.
31767225	7	67	theme	glycans	1743:1749	arg1	recovery					1731:1738	quantitative recovery	1718:1738	quantitative recovery of glycans from selected glycoproteins	1718:1777	Capillary electrophoresis (CE) of released glycans labeled with 8-aminopyrene-1,3,6-trisulfonate (APTS) demonstrated quantitative recovery of glycans from selected glycoproteins.
31767225	3	68	link	N-linked	618:625	arg1	glycans					627:633	all N-linked glycans	614:633	all N-linked glycans	614:633	The commonly-used in-solution deglycosylation method is relatively time-consuming and requires several hours up to overnight for complete removal of all N-linked glycans.
31767225	5	69	theme	photocatalytic	1167:1180	arg1	acrylamide					1031:1040	acrylamide	1031:1040	acrylamide	1031:1040	Our enzyme reactor is based on simple photochemical copolymerization of monomers using the following procedure: (1) a pipette tip was filled with a gel solution comprising acrylamide, N,N'-methylene-bis-acrylamide containing PNGase F or trypsin with 2,2-azobis(2-methyl-N-(2-hydroxyethyl) propionamide) as a photocatalytic initiator; and (2) in situ polymerization of gel solution approximately 30 mm from the tip was performed by irradiation with a 365 nm blue LED beam from a distance 10 mm.
31767225	5	69	theme	photocatalytic	1167:1180	arg1	initiator					1182:1190	a photocatalytic initiator	1165:1190	a photocatalytic initiator	1165:1190	Our enzyme reactor is based on simple photochemical copolymerization of monomers using the following procedure: (1) a pipette tip was filled with a gel solution comprising acrylamide, N,N'-methylene-bis-acrylamide containing PNGase F or trypsin with 2,2-azobis(2-methyl-N-(2-hydroxyethyl) propionamide) as a photocatalytic initiator; and (2) in situ polymerization of gel solution approximately 30 mm from the tip was performed by irradiation with a 365 nm blue LED beam from a distance 10 mm.
31767225	0	70	theme	-impregnated	149:160	arg1	gels					177:180	trypsin- and peptide-N-glycosidase F (PNGase F)-impregnated polyacrylamide gels	102:180	trypsin- and peptide-N-glycosidase F (PNGase F)-impregnated polyacrylamide gels fabricated in a pipette tip	102:208	A fast and convenient solid phase preparation method for releasing N-glycans from glycoproteins using trypsin- and peptide-N-glycosidase F (PNGase F)-impregnated polyacrylamide gels fabricated in a pipette tip.
31767225	6	71	theme	fixed	1357:1361	arg1	enzymes					1363:1369	The fixed enzymes	1353:1369	The fixed enzymes	1353:1369	The fixed enzymes maintained their activities in the polyacrylamide gel and the reaction was completed by 40 iterations of suction and discharge with a pipette (hereafter referred to as manual pipetting times) for 8 min with each enzyme digestion.
31767225	1	72	theme	antibodies	384:393	arg1	purification					356:367	purification	356:367	purification	356:367	An efficient deglycosylation process is a key requirement for the identification and characterization of glycosylation during the production and purification of therapeutic antibodies.
31767225	1	72	theme	antibodies	384:393	arg1	production					341:350	production	341:350	production	341:350	An efficient deglycosylation process is a key requirement for the identification and characterization of glycosylation during the production and purification of therapeutic antibodies.
31767225	1	73	theme	deglycosylation	224:238	arg1	process					240:246	An efficient deglycosylation process	211:246	An efficient deglycosylation process	211:246	An efficient deglycosylation process is a key requirement for the identification and characterization of glycosylation during the production and purification of therapeutic antibodies.
31767225	1	73	theme	deglycosylation	224:238	arg1	requirement					257:267	a key requirement	251:267	a key requirement for the identification and characterization of glycosylation	251:328	An efficient deglycosylation process is a key requirement for the identification and characterization of glycosylation during the production and purification of therapeutic antibodies.
31767225	0	74	theme	fast	2:5	arg1	method					46:51	A fast and convenient solid phase preparation method	0:51	A fast and convenient solid phase preparation method for releasing N-glycans from glycoproteins using trypsin- and peptide-N-glycosidase F (PNGase F)-impregnated polyacrylamide gels fabricated in a pipette tip.	0:209	A fast and convenient solid phase preparation method for releasing N-glycans from glycoproteins using trypsin- and peptide-N-glycosidase F (PNGase F)-impregnated polyacrylamide gels fabricated in a pipette tip.
31767225	0	75	gly	glycoproteins	82:94	arg1	glycoproteins					82:94	glycoproteins	82:94	glycoproteins using trypsin- and peptide-N-glycosidase F (PNGase F)-impregnated polyacrylamide gels fabricated in a pipette tip	82:208	A fast and convenient solid phase preparation method for releasing N-glycans from glycoproteins using trypsin- and peptide-N-glycosidase F (PNGase F)-impregnated polyacrylamide gels fabricated in a pipette tip.
31767225	5	76	theme	2-hydroxyethyl	1132:1145	arg1	2,2-azobis					1109:1118	2,2-azobis	1109:1118	2,2-azobis(2-methyl-N-(2-hydroxyethyl) propionamide)	1109:1160	Our enzyme reactor is based on simple photochemical copolymerization of monomers using the following procedure: (1) a pipette tip was filled with a gel solution comprising acrylamide, N,N'-methylene-bis-acrylamide containing PNGase F or trypsin with 2,2-azobis(2-methyl-N-(2-hydroxyethyl) propionamide) as a photocatalytic initiator; and (2) in situ polymerization of gel solution approximately 30 mm from the tip was performed by irradiation with a 365 nm blue LED beam from a distance 10 mm.
31767225	5	76	theme	2-hydroxyethyl	1132:1145	arg1	propionamide					1148:1159	2-methyl-N-(2-hydroxyethyl) propionamide	1120:1159	2-methyl-N-(2-hydroxyethyl) propionamide	1120:1159	Our enzyme reactor is based on simple photochemical copolymerization of monomers using the following procedure: (1) a pipette tip was filled with a gel solution comprising acrylamide, N,N'-methylene-bis-acrylamide containing PNGase F or trypsin with 2,2-azobis(2-methyl-N-(2-hydroxyethyl) propionamide) as a photocatalytic initiator; and (2) in situ polymerization of gel solution approximately 30 mm from the tip was performed by irradiation with a 365 nm blue LED beam from a distance 10 mm.
31767225	5	77	theme	blue	1316:1319	arg1	365 nm					1309:1314	a 365 nm blue	1307:1319	a 365 nm blue LED beam from a distance 10 mm	1307:1350	Our enzyme reactor is based on simple photochemical copolymerization of monomers using the following procedure: (1) a pipette tip was filled with a gel solution comprising acrylamide, N,N'-methylene-bis-acrylamide containing PNGase F or trypsin with 2,2-azobis(2-methyl-N-(2-hydroxyethyl) propionamide) as a photocatalytic initiator; and (2) in situ polymerization of gel solution approximately 30 mm from the tip was performed by irradiation with a 365 nm blue LED beam from a distance 10 mm.
31767225	0	78	theme	convenient	11:20	arg1	method					46:51	A fast and convenient solid phase preparation method	0:51	A fast and convenient solid phase preparation method for releasing N-glycans from glycoproteins using trypsin- and peptide-N-glycosidase F (PNGase F)-impregnated polyacrylamide gels fabricated in a pipette tip.	0:209	A fast and convenient solid phase preparation method for releasing N-glycans from glycoproteins using trypsin- and peptide-N-glycosidase F (PNGase F)-impregnated polyacrylamide gels fabricated in a pipette tip.
31767225	2	79	theme	N-linked	447:454	arg1	glycans					456:462	N-linked glycans	447:462	N-linked glycans	447:462	PNGase F is widely used for the deglycosylation of N-linked glycans.
31767225	1	80	dep	production	341:350	arg1	the					337:339	the	337:339	the	337:339	An efficient deglycosylation process is a key requirement for the identification and characterization of glycosylation during the production and purification of therapeutic antibodies.
31767225	4	81	theme	F-impregnated	782:794	arg1	gels					811:814	trypsin- and PNGase F-impregnated polyacrylamide gels	762:814	trypsin- and PNGase F-impregnated polyacrylamide gels	762:814	In order to develop a simple and efficient method for the rapid release of N-linked glycans from glycoproteins, we fabricated trypsin- and PNGase F-impregnated polyacrylamide gels in a commercial 200 μL volume pipette tip.
31767225	0	82	theme	phase	28:32	arg1	method					46:51	A fast and convenient solid phase preparation method	0:51	A fast and convenient solid phase preparation method for releasing N-glycans from glycoproteins using trypsin- and peptide-N-glycosidase F (PNGase F)-impregnated polyacrylamide gels fabricated in a pipette tip.	0:209	A fast and convenient solid phase preparation method for releasing N-glycans from glycoproteins using trypsin- and peptide-N-glycosidase F (PNGase F)-impregnated polyacrylamide gels fabricated in a pipette tip.
31767225	5	83	theme	situ	1204:1207	arg1	polymerization					1209:1222	situ polymerization	1204:1222	situ polymerization of gel solution approximately 30 mm from the tip	1204:1271	Our enzyme reactor is based on simple photochemical copolymerization of monomers using the following procedure: (1) a pipette tip was filled with a gel solution comprising acrylamide, N,N'-methylene-bis-acrylamide containing PNGase F or trypsin with 2,2-azobis(2-methyl-N-(2-hydroxyethyl) propionamide) as a photocatalytic initiator; and (2) in situ polymerization of gel solution approximately 30 mm from the tip was performed by irradiation with a 365 nm blue LED beam from a distance 10 mm.
31767225	3	84	theme	in-solution	483:493	arg1	time-consuming					532:545	time-consuming	532:545	time-consuming	532:545	The commonly-used in-solution deglycosylation method is relatively time-consuming and requires several hours up to overnight for complete removal of all N-linked glycans.
31767225	3	84	theme	in-solution	483:493	arg1	method					511:516	The commonly-used in-solution deglycosylation method	465:516	The commonly-used in-solution deglycosylation method	465:516	The commonly-used in-solution deglycosylation method is relatively time-consuming and requires several hours up to overnight for complete removal of all N-linked glycans.
31767225	1	85	theme	glycosylation	316:328	arg1	characterization					296:311	characterization	296:311	characterization	296:311	An efficient deglycosylation process is a key requirement for the identification and characterization of glycosylation during the production and purification of therapeutic antibodies.
31767225	1	85	theme	glycosylation	316:328	arg1	identification					277:290	identification	277:290	identification	277:290	An efficient deglycosylation process is a key requirement for the identification and characterization of glycosylation during the production and purification of therapeutic antibodies.
31767225	4	86	theme	pipette	846:852	arg1	tip					854:856	a commercial 200 μL volume pipette tip	819:856	a commercial 200 μL volume pipette tip	819:856	In order to develop a simple and efficient method for the rapid release of N-linked glycans from glycoproteins, we fabricated trypsin- and PNGase F-impregnated polyacrylamide gels in a commercial 200 μL volume pipette tip.
31767225	7	87	gly	glycoproteins	1765:1777	arg1	glycoproteins					1765:1777	selected glycoproteins	1756:1777	selected glycoproteins	1756:1777	Capillary electrophoresis (CE) of released glycans labeled with 8-aminopyrene-1,3,6-trisulfonate (APTS) demonstrated quantitative recovery of glycans from selected glycoproteins.
31767225	4	88	theme	200 μL	832:837	arg1	tip					854:856	a commercial 200 μL volume pipette tip	819:856	a commercial 200 μL volume pipette tip	819:856	In order to develop a simple and efficient method for the rapid release of N-linked glycans from glycoproteins, we fabricated trypsin- and PNGase F-impregnated polyacrylamide gels in a commercial 200 μL volume pipette tip.
31767225	6	89	theme	discharge	1488:1496	arg1	iterations					1462:1471	40 iterations	1459:1471	40 iterations of suction and discharge with a pipette (hereafter referred to as manual pipetting times) for 8 min with each enzyme digestion	1459:1598	The fixed enzymes maintained their activities in the polyacrylamide gel and the reaction was completed by 40 iterations of suction and discharge with a pipette (hereafter referred to as manual pipetting times) for 8 min with each enzyme digestion.
31767225	5	90	theme	30 mm	1254:1258	arg1	solution					1231:1238	gel solution	1227:1238	gel solution approximately 30 mm from the tip	1227:1271	Our enzyme reactor is based on simple photochemical copolymerization of monomers using the following procedure: (1) a pipette tip was filled with a gel solution comprising acrylamide, N,N'-methylene-bis-acrylamide containing PNGase F or trypsin with 2,2-azobis(2-methyl-N-(2-hydroxyethyl) propionamide) as a photocatalytic initiator; and (2) in situ polymerization of gel solution approximately 30 mm from the tip was performed by irradiation with a 365 nm blue LED beam from a distance 10 mm.
31767225	4	91	from	glycoproteins	733:745	arg1	release					700:706	the rapid release	690:706	the rapid release of N-linked glycans from glycoproteins	690:745	In order to develop a simple and efficient method for the rapid release of N-linked glycans from glycoproteins, we fabricated trypsin- and PNGase F-impregnated polyacrylamide gels in a commercial 200 μL volume pipette tip.
31767225	4	92	theme	simple	658:663	arg1	method					679:684	a simple and efficient method	656:684	a simple and efficient method for the rapid release of N-linked glycans from glycoproteins	656:745	In order to develop a simple and efficient method for the rapid release of N-linked glycans from glycoproteins, we fabricated trypsin- and PNGase F-impregnated polyacrylamide gels in a commercial 200 μL volume pipette tip.
31767225	5	93	dep	based	881:885	arg1	performed					1277:1285	performed	1277:1285	was performed by irradiation with a 365 nm blue LED beam from a distance 10 mm	1273:1350	Our enzyme reactor is based on simple photochemical copolymerization of monomers using the following procedure: (1) a pipette tip was filled with a gel solution comprising acrylamide, N,N'-methylene-bis-acrylamide containing PNGase F or trypsin with 2,2-azobis(2-methyl-N-(2-hydroxyethyl) propionamide) as a photocatalytic initiator; and (2) in situ polymerization of gel solution approximately 30 mm from the tip was performed by irradiation with a 365 nm blue LED beam from a distance 10 mm.
31767225	5	93	dep	based	881:885	arg1	in					1201:1202	in	1201:1202	in	1201:1202	Our enzyme reactor is based on simple photochemical copolymerization of monomers using the following procedure: (1) a pipette tip was filled with a gel solution comprising acrylamide, N,N'-methylene-bis-acrylamide containing PNGase F or trypsin with 2,2-azobis(2-methyl-N-(2-hydroxyethyl) propionamide) as a photocatalytic initiator; and (2) in situ polymerization of gel solution approximately 30 mm from the tip was performed by irradiation with a 365 nm blue LED beam from a distance 10 mm.
31767225	6	94	theme	suction	1476:1482	arg1	iterations					1462:1471	40 iterations	1459:1471	40 iterations of suction and discharge with a pipette (hereafter referred to as manual pipetting times) for 8 min with each enzyme digestion	1459:1598	The fixed enzymes maintained their activities in the polyacrylamide gel and the reaction was completed by 40 iterations of suction and discharge with a pipette (hereafter referred to as manual pipetting times) for 8 min with each enzyme digestion.
29395289	3	0	theme	moieties	467:474	arg1	significance					361:372	the significance	357:372	the significance of Fc receptors (FcRs), actin filament polymerization, mannose receptors (MRs), carbohydrate moieties like N-linked glycans and sialic acid on chicken macrophages for invasion of S. Typhimurium	357:566	The objective of this study was to investigate the significance of Fc receptors (FcRs), actin filament polymerization, mannose receptors (MRs), carbohydrate moieties like N-linked glycans and sialic acid on chicken macrophages for invasion of S. Typhimurium.
29395289	6	1	theme	Typhimurium	868:878	arg1	invasion					880:887	S. Typhimurium invasion	865:887	S. Typhimurium invasion	865:887	Pre-incubation of macrophages with cytochalasin D, showed severe decrease (28 folds) in S. Typhimurium invasion.
29395289	3	2	theme	acid	509:512	arg1	significance					361:372	the significance	357:372	the significance of Fc receptors (FcRs), actin filament polymerization, mannose receptors (MRs), carbohydrate moieties like N-linked glycans and sialic acid on chicken macrophages for invasion of S. Typhimurium	357:566	The objective of this study was to investigate the significance of Fc receptors (FcRs), actin filament polymerization, mannose receptors (MRs), carbohydrate moieties like N-linked glycans and sialic acid on chicken macrophages for invasion of S. Typhimurium.
29395289	1	3	theme	pathogen	164:171	arg1	entry					173:177	pathogen entry	164:177	pathogen entry	164:177	Bacterial attachment to host cell is the first event for pathogen entry.
29395289	8	4	with	Treatment	997:1005	arg1	neuraminidase					1068:1080	neuraminidase	1068:1080	neuraminidase	1068:1080	Treatment of macrophages with methyl α-d-mannopyranoside, PNGase F and neuraminidase, showed 2.5, 5 and 2.5 folds decrease in invasion respectively.
29395289	8	4	with	Treatment	997:1005	arg1	α-d-mannopyranoside					1034:1052	methyl α-d-mannopyranoside	1027:1052	methyl α-d-mannopyranoside	1027:1052	Treatment of macrophages with methyl α-d-mannopyranoside, PNGase F and neuraminidase, showed 2.5, 5 and 2.5 folds decrease in invasion respectively.
29395289	8	4	with	Treatment	997:1005	arg1	F					1062:1062	PNGase F	1055:1062	PNGase F	1055:1062	Treatment of macrophages with methyl α-d-mannopyranoside, PNGase F and neuraminidase, showed 2.5, 5 and 2.5 folds decrease in invasion respectively.
29395289	6	5	with	Pre-incubation	777:790	arg1	D					825:825	cytochalasin D	812:825	cytochalasin D	812:825	Pre-incubation of macrophages with cytochalasin D, showed severe decrease (28 folds) in S. Typhimurium invasion.
29395289	9	6	theme	N-linked	1187:1194	arg1	acid					1221:1224	sialic acid	1214:1224	sialic acid	1214:1224	Our data suggest that deglycosylation of N-linked glycans including sialic acid by PNGase F is more effective in inhibition of S. Typhimurium invasion than neuraminidase which removes only sialic acid.
29395289	9	6	theme	N-linked	1187:1194	arg1	glycans					1196:1202	N-linked glycans	1187:1202	N-linked glycans including sialic acid	1187:1224	Our data suggest that deglycosylation of N-linked glycans including sialic acid by PNGase F is more effective in inhibition of S. Typhimurium invasion than neuraminidase which removes only sialic acid.
29395289	5	7	theme	Cytochalasin	679:690	arg1	D					692:692	Cytochalasin D	679:692	Cytochalasin D	679:692	Cytochalasin D, an inhibitor of actin filament polymerization prevented uptake of S. Typhimurium.
29395289	5	7	theme	Cytochalasin	679:690	arg1	inhibitor					698:706	an inhibitor	695:706	an inhibitor of actin filament polymerization	695:739	Cytochalasin D, an inhibitor of actin filament polymerization prevented uptake of S. Typhimurium.
29395289	3	8	theme	chicken	517:523	arg1	macrophages					525:535	chicken macrophages	517:535	chicken macrophages	517:535	The objective of this study was to investigate the significance of Fc receptors (FcRs), actin filament polymerization, mannose receptors (MRs), carbohydrate moieties like N-linked glycans and sialic acid on chicken macrophages for invasion of S. Typhimurium.
29395289	9	9	from	inhibition	1259:1268	arg1	effective					1246:1254	effective	1246:1254	effective	1246:1254	Our data suggest that deglycosylation of N-linked glycans including sialic acid by PNGase F is more effective in inhibition of S. Typhimurium invasion than neuraminidase which removes only sialic acid.
29395289	0	10	from	invasion	74:81	arg1	macrophages					94:104	chicken macrophages	86:104	chicken macrophages	86:104	Role of different receptors and actin filaments on Salmonella Typhimurium invasion in chicken macrophages.
29395289	2	11	theme	expressed	224:232	arg1	adhesins					234:241	membrane expressed adhesins	215:241	membrane expressed adhesins present on the organism	215:265	The attachment is mediated through membrane expressed adhesins present on the organism and receptors on the cell surface of host.
29395289	9	12	from	effective	1246:1254	arg1	inhibition					1259:1268	inhibition	1259:1268	inhibition of S. Typhimurium invasion	1259:1295	Our data suggest that deglycosylation of N-linked glycans including sialic acid by PNGase F is more effective in inhibition of S. Typhimurium invasion than neuraminidase which removes only sialic acid.
29395289	9	13	theme	PNGase	1229:1234	arg1	F					1236:1236	PNGase F	1229:1236	PNGase F	1229:1236	Our data suggest that deglycosylation of N-linked glycans including sialic acid by PNGase F is more effective in inhibition of S. Typhimurium invasion than neuraminidase which removes only sialic acid.
29395289	7	14	theme	S.	972:973	arg1	invasion					987:994	S. Typhimurium invasion	972:994	S. Typhimurium invasion	972:994	Next we attempted to analyse the role of carbohydrate receptors of macrophages in S. Typhimurium invasion.
29395289	2	15	from	receptors	271:279	arg1	surface					293:299	the cell surface	284:299	the cell surface of host	284:307	The attachment is mediated through membrane expressed adhesins present on the organism and receptors on the cell surface of host.
29395289	3	16	from	moieties	467:474	arg1	macrophages					525:535	chicken macrophages	517:535	chicken macrophages	517:535	The objective of this study was to investigate the significance of Fc receptors (FcRs), actin filament polymerization, mannose receptors (MRs), carbohydrate moieties like N-linked glycans and sialic acid on chicken macrophages for invasion of S. Typhimurium.
29395289	7	17	theme	macrophages	957:967	arg1	receptors					944:952	carbohydrate receptors	931:952	carbohydrate receptors of macrophages	931:967	Next we attempted to analyse the role of carbohydrate receptors of macrophages in S. Typhimurium invasion.
29395289	10	18	link	N-linked	1415:1422	arg1	glycans					1424:1430	N-linked glycans	1415:1430	N-linked glycans	1415:1430	These findings suggested FcRs, actin filament polymerization, MRs, N-linked glycans and sialic acid may act as gateway for entry of S. Typhimurium.
29395289	5	19	theme	polymerization	726:739	arg1	D					692:692	Cytochalasin D	679:692	Cytochalasin D	679:692	Cytochalasin D, an inhibitor of actin filament polymerization prevented uptake of S. Typhimurium.
29395289	5	19	theme	polymerization	726:739	arg1	inhibitor					698:706	an inhibitor	695:706	an inhibitor of actin filament polymerization	695:739	Cytochalasin D, an inhibitor of actin filament polymerization prevented uptake of S. Typhimurium.
29395289	2	20	theme	present	243:249	arg1	adhesins					234:241	membrane expressed adhesins	215:241	membrane expressed adhesins present on the organism	215:265	The attachment is mediated through membrane expressed adhesins present on the organism and receptors on the cell surface of host.
29395289	7	21	theme	receptors	944:952	arg1	role					923:926	the role	919:926	the role of carbohydrate receptors of macrophages in S. Typhimurium invasion	919:994	Next we attempted to analyse the role of carbohydrate receptors of macrophages in S. Typhimurium invasion.
29395289	0	22	from	Role	0:3	arg1	invasion					74:81	Salmonella Typhimurium invasion	51:81	Salmonella Typhimurium invasion in chicken macrophages	51:104	Role of different receptors and actin filaments on Salmonella Typhimurium invasion in chicken macrophages.
29395289	1	23	theme	Bacterial	107:115	arg1	attachment					117:126	Bacterial attachment	107:126	Bacterial attachment to host cell	107:139	Bacterial attachment to host cell is the first event for pathogen entry.
29395289	1	23	theme	Bacterial	107:115	arg1	event					154:158	the first event	144:158	the first event for pathogen entry	144:177	Bacterial attachment to host cell is the first event for pathogen entry.
29395289	0	24	theme	chicken	86:92	arg1	macrophages					94:104	chicken macrophages	86:104	chicken macrophages	86:104	Role of different receptors and actin filaments on Salmonella Typhimurium invasion in chicken macrophages.
29395289	9	25	theme	S.	1273:1274	arg1	invasion					1288:1295	S. Typhimurium invasion	1273:1295	S. Typhimurium invasion	1273:1295	Our data suggest that deglycosylation of N-linked glycans including sialic acid by PNGase F is more effective in inhibition of S. Typhimurium invasion than neuraminidase which removes only sialic acid.
29395289	8	26	theme	macrophages	1010:1020	arg1	Treatment					997:1005	Treatment	997:1005	Treatment	997:1005	Treatment of macrophages with methyl α-d-mannopyranoside, PNGase F and neuraminidase, showed 2.5, 5 and 2.5 folds decrease in invasion respectively.
29395289	4	27	dep	derived	658:664	arg1	monocyte					649:656	monocyte	649:656	monocyte	649:656	Opsonisation of S. Typhimurium resulted in three folds more invasion in chicken monocyte derived macrophages.
29395289	3	28	theme	actin	398:402	arg1	polymerization					413:426	actin filament polymerization	398:426	actin filament polymerization	398:426	The objective of this study was to investigate the significance of Fc receptors (FcRs), actin filament polymerization, mannose receptors (MRs), carbohydrate moieties like N-linked glycans and sialic acid on chicken macrophages for invasion of S. Typhimurium.
29395289	5	29	theme	actin	711:715	arg1	polymerization					726:739	actin filament polymerization	711:739	actin filament polymerization	711:739	Cytochalasin D, an inhibitor of actin filament polymerization prevented uptake of S. Typhimurium.
29395289	3	30	from	significance	361:372	arg1	macrophages					525:535	chicken macrophages	517:535	chicken macrophages	517:535	The objective of this study was to investigate the significance of Fc receptors (FcRs), actin filament polymerization, mannose receptors (MRs), carbohydrate moieties like N-linked glycans and sialic acid on chicken macrophages for invasion of S. Typhimurium.
29395289	8	31	theme	methyl	1027:1032	arg1	α-d-mannopyranoside					1034:1052	methyl α-d-mannopyranoside	1027:1052	methyl α-d-mannopyranoside	1027:1052	Treatment of macrophages with methyl α-d-mannopyranoside, PNGase F and neuraminidase, showed 2.5, 5 and 2.5 folds decrease in invasion respectively.
29395289	2	32	from	adhesins	234:241	arg1	surface					293:299	the cell surface	284:299	the cell surface of host	284:307	The attachment is mediated through membrane expressed adhesins present on the organism and receptors on the cell surface of host.
29395289	10	33	theme	sialic	1436:1441	arg1	acid					1443:1446	sialic acid	1436:1446	sialic acid	1436:1446	These findings suggested FcRs, actin filament polymerization, MRs, N-linked glycans and sialic acid may act as gateway for entry of S. Typhimurium.
29395289	3	34	theme	mannose	429:435	arg1	MRs					448:450	MRs	448:450	MRs	448:450	The objective of this study was to investigate the significance of Fc receptors (FcRs), actin filament polymerization, mannose receptors (MRs), carbohydrate moieties like N-linked glycans and sialic acid on chicken macrophages for invasion of S. Typhimurium.
29395289	3	34	theme	mannose	429:435	arg1	receptors					437:445	mannose receptors	429:445	mannose receptors (MRs)	429:451	The objective of this study was to investigate the significance of Fc receptors (FcRs), actin filament polymerization, mannose receptors (MRs), carbohydrate moieties like N-linked glycans and sialic acid on chicken macrophages for invasion of S. Typhimurium.
29395289	0	35	theme	different	8:16	arg1	receptors					18:26	different receptors	8:26	different receptors	8:26	Role of different receptors and actin filaments on Salmonella Typhimurium invasion in chicken macrophages.
29395289	8	36	from	decrease	1111:1118	arg1	invasion					1123:1130	invasion	1123:1130	invasion	1123:1130	Treatment of macrophages with methyl α-d-mannopyranoside, PNGase F and neuraminidase, showed 2.5, 5 and 2.5 folds decrease in invasion respectively.
29395289	0	37	dep	Salmonella	51:60	arg1	Typhimurium					62:72	Typhimurium	62:72	Typhimurium	62:72	Role of different receptors and actin filaments on Salmonella Typhimurium invasion in chicken macrophages.
29395289	3	38	theme	polymerization	413:426	arg1	significance					361:372	the significance	357:372	the significance of Fc receptors (FcRs), actin filament polymerization, mannose receptors (MRs), carbohydrate moieties like N-linked glycans and sialic acid on chicken macrophages for invasion of S. Typhimurium	357:566	The objective of this study was to investigate the significance of Fc receptors (FcRs), actin filament polymerization, mannose receptors (MRs), carbohydrate moieties like N-linked glycans and sialic acid on chicken macrophages for invasion of S. Typhimurium.
29395289	9	39	theme	sialic	1335:1340	arg1	acid					1342:1345	only sialic acid	1330:1345	only sialic acid	1330:1345	Our data suggest that deglycosylation of N-linked glycans including sialic acid by PNGase F is more effective in inhibition of S. Typhimurium invasion than neuraminidase which removes only sialic acid.
29395289	3	40	theme	Fc	377:378	arg1	receptors					380:388	Fc receptors	377:388	Fc receptors (FcRs)	377:395	The objective of this study was to investigate the significance of Fc receptors (FcRs), actin filament polymerization, mannose receptors (MRs), carbohydrate moieties like N-linked glycans and sialic acid on chicken macrophages for invasion of S. Typhimurium.
29395289	3	40	theme	Fc	377:378	arg1	FcRs					391:394	FcRs	391:394	FcRs	391:394	The objective of this study was to investigate the significance of Fc receptors (FcRs), actin filament polymerization, mannose receptors (MRs), carbohydrate moieties like N-linked glycans and sialic acid on chicken macrophages for invasion of S. Typhimurium.
29395289	10	41	theme	Typhimurium	1483:1493	arg1	entry					1471:1475	entry	1471:1475	entry of S. Typhimurium	1471:1493	These findings suggested FcRs, actin filament polymerization, MRs, N-linked glycans and sialic acid may act as gateway for entry of S. Typhimurium.
29395289	0	42	theme	Salmonella	51:60	arg1	invasion					74:81	Salmonella Typhimurium invasion	51:81	Salmonella Typhimurium invasion in chicken macrophages	51:104	Role of different receptors and actin filaments on Salmonella Typhimurium invasion in chicken macrophages.
29395289	0	43	theme	filaments	38:46	arg1	Role					0:3	Role	0:3	Role of different receptors and actin filaments on Salmonella Typhimurium invasion in chicken macrophages.	0:105	Role of different receptors and actin filaments on Salmonella Typhimurium invasion in chicken macrophages.
29395289	3	44	from	receptors	437:445	arg1	macrophages					525:535	chicken macrophages	517:535	chicken macrophages	517:535	The objective of this study was to investigate the significance of Fc receptors (FcRs), actin filament polymerization, mannose receptors (MRs), carbohydrate moieties like N-linked glycans and sialic acid on chicken macrophages for invasion of S. Typhimurium.
29395289	4	45	theme	chicken	641:647	arg1	macrophages					666:676	chicken monocyte derived macrophages	641:676	chicken monocyte derived macrophages	641:676	Opsonisation of S. Typhimurium resulted in three folds more invasion in chicken monocyte derived macrophages.
29395289	3	46	theme	carbohydrate	454:465	arg1	moieties					467:474	carbohydrate moieties	454:474	carbohydrate moieties like N-linked glycans	454:496	The objective of this study was to investigate the significance of Fc receptors (FcRs), actin filament polymerization, mannose receptors (MRs), carbohydrate moieties like N-linked glycans and sialic acid on chicken macrophages for invasion of S. Typhimurium.
29395289	6	47	theme	macrophages	795:805	arg1	Pre-incubation					777:790	Pre-incubation	777:790	Pre-incubation	777:790	Pre-incubation of macrophages with cytochalasin D, showed severe decrease (28 folds) in S. Typhimurium invasion.
29395289	3	48	theme	sialic	502:507	arg1	acid					509:512	sialic acid	502:512	sialic acid on chicken macrophages	502:535	The objective of this study was to investigate the significance of Fc receptors (FcRs), actin filament polymerization, mannose receptors (MRs), carbohydrate moieties like N-linked glycans and sialic acid on chicken macrophages for invasion of S. Typhimurium.
29395289	6	49	theme	S.	865:866	arg1	invasion					880:887	S. Typhimurium invasion	865:887	S. Typhimurium invasion	865:887	Pre-incubation of macrophages with cytochalasin D, showed severe decrease (28 folds) in S. Typhimurium invasion.
29395289	2	50	theme	host	304:307	arg1	surface					293:299	the cell surface	284:299	the cell surface of host	284:307	The attachment is mediated through membrane expressed adhesins present on the organism and receptors on the cell surface of host.
29395289	4	51	link	derived	658:664	arg1	macrophages					666:676	chicken monocyte derived macrophages	641:676	chicken monocyte derived macrophages	641:676	Opsonisation of S. Typhimurium resulted in three folds more invasion in chicken monocyte derived macrophages.
29395289	8	52	dep	2.5	1090:1092	arg1	folds					1105:1109	folds	1105:1109	folds decrease in invasion	1105:1130	Treatment of macrophages with methyl α-d-mannopyranoside, PNGase F and neuraminidase, showed 2.5, 5 and 2.5 folds decrease in invasion respectively.
29395289	10	53	theme	filament	1385:1392	arg1	polymerization					1394:1407	actin filament polymerization	1379:1407	actin filament polymerization	1379:1407	These findings suggested FcRs, actin filament polymerization, MRs, N-linked glycans and sialic acid may act as gateway for entry of S. Typhimurium.
29395289	9	54	theme	glycans	1196:1202	arg1	deglycosylation					1168:1182	deglycosylation	1168:1182	deglycosylation of N-linked glycans including sialic acid by PNGase F	1168:1236	Our data suggest that deglycosylation of N-linked glycans including sialic acid by PNGase F is more effective in inhibition of S. Typhimurium invasion than neuraminidase which removes only sialic acid.
29395289	4	55	theme	Typhimurium	588:598	arg1	Opsonisation					569:580	Opsonisation	569:580	Opsonisation of S. Typhimurium	569:598	Opsonisation of S. Typhimurium resulted in three folds more invasion in chicken monocyte derived macrophages.
29395289	9	56	theme	sialic	1214:1219	arg1	acid					1221:1224	sialic acid	1214:1224	sialic acid	1214:1224	Our data suggest that deglycosylation of N-linked glycans including sialic acid by PNGase F is more effective in inhibition of S. Typhimurium invasion than neuraminidase which removes only sialic acid.
29395289	9	57	gly	deglycosylation	1168:1182	arg1	acid					1221:1224	sialic acid	1214:1224	sialic acid	1214:1224	Our data suggest that deglycosylation of N-linked glycans including sialic acid by PNGase F is more effective in inhibition of S. Typhimurium invasion than neuraminidase which removes only sialic acid.
29395289	9	57	gly	deglycosylation	1168:1182	arg1	glycans					1196:1202	N-linked glycans	1187:1202	N-linked glycans including sialic acid	1187:1224	Our data suggest that deglycosylation of N-linked glycans including sialic acid by PNGase F is more effective in inhibition of S. Typhimurium invasion than neuraminidase which removes only sialic acid.
29395289	3	58	link	N-linked	481:488	arg1	glycans					490:496	N-linked glycans	481:496	N-linked glycans	481:496	The objective of this study was to investigate the significance of Fc receptors (FcRs), actin filament polymerization, mannose receptors (MRs), carbohydrate moieties like N-linked glycans and sialic acid on chicken macrophages for invasion of S. Typhimurium.
29395289	2	59	dep	expressed	224:232	arg1	membrane					215:222	membrane	215:222	membrane	215:222	The attachment is mediated through membrane expressed adhesins present on the organism and receptors on the cell surface of host.
29395289	10	60	theme	actin	1379:1383	arg1	polymerization					1394:1407	actin filament polymerization	1379:1407	actin filament polymerization	1379:1407	These findings suggested FcRs, actin filament polymerization, MRs, N-linked glycans and sialic acid may act as gateway for entry of S. Typhimurium.
29395289	7	61	theme	Typhimurium	975:985	arg1	invasion					987:994	S. Typhimurium invasion	972:994	S. Typhimurium invasion	972:994	Next we attempted to analyse the role of carbohydrate receptors of macrophages in S. Typhimurium invasion.
29395289	1	62	theme	host	131:134	arg1	cell					136:139	host cell	131:139	host cell	131:139	Bacterial attachment to host cell is the first event for pathogen entry.
29395289	3	63	from	macrophages	525:535	arg1	significance					361:372	the significance	357:372	the significance of Fc receptors (FcRs), actin filament polymerization, mannose receptors (MRs), carbohydrate moieties like N-linked glycans and sialic acid on chicken macrophages for invasion of S. Typhimurium	357:566	The objective of this study was to investigate the significance of Fc receptors (FcRs), actin filament polymerization, mannose receptors (MRs), carbohydrate moieties like N-linked glycans and sialic acid on chicken macrophages for invasion of S. Typhimurium.
29395289	5	64	theme	filament	717:724	arg1	polymerization					726:739	actin filament polymerization	711:739	actin filament polymerization	711:739	Cytochalasin D, an inhibitor of actin filament polymerization prevented uptake of S. Typhimurium.
29395289	3	65	from	acid	509:512	arg1	macrophages					525:535	chicken macrophages	517:535	chicken macrophages	517:535	The objective of this study was to investigate the significance of Fc receptors (FcRs), actin filament polymerization, mannose receptors (MRs), carbohydrate moieties like N-linked glycans and sialic acid on chicken macrophages for invasion of S. Typhimurium.
29395289	2	66	attach	present	243:249	arg2	adhesins					234:241	membrane expressed adhesins	215:241	membrane expressed adhesins present on the organism	215:265	The attachment is mediated through membrane expressed adhesins present on the organism and receptors on the cell surface of host.
29395289	2	66	attach	present	243:249	arg1	organism					258:265	the organism	254:265	the organism	254:265	The attachment is mediated through membrane expressed adhesins present on the organism and receptors on the cell surface of host.
29395289	7	67	from	role	923:926	arg1	invasion					987:994	S. Typhimurium invasion	972:994	S. Typhimurium invasion	972:994	Next we attempted to analyse the role of carbohydrate receptors of macrophages in S. Typhimurium invasion.
29395289	3	68	from	receptors	380:388	arg1	macrophages					525:535	chicken macrophages	517:535	chicken macrophages	517:535	The objective of this study was to investigate the significance of Fc receptors (FcRs), actin filament polymerization, mannose receptors (MRs), carbohydrate moieties like N-linked glycans and sialic acid on chicken macrophages for invasion of S. Typhimurium.
29395289	1	69	theme	first	148:152	arg1	attachment					117:126	Bacterial attachment	107:126	Bacterial attachment to host cell	107:139	Bacterial attachment to host cell is the first event for pathogen entry.
29395289	1	69	theme	first	148:152	arg1	event					154:158	the first event	144:158	the first event for pathogen entry	144:177	Bacterial attachment to host cell is the first event for pathogen entry.
29395289	10	70	theme	N-linked	1415:1422	arg1	glycans					1424:1430	N-linked glycans	1415:1430	N-linked glycans	1415:1430	These findings suggested FcRs, actin filament polymerization, MRs, N-linked glycans and sialic acid may act as gateway for entry of S. Typhimurium.
29395289	4	71	theme	derived	658:664	arg1	macrophages					666:676	chicken monocyte derived macrophages	641:676	chicken monocyte derived macrophages	641:676	Opsonisation of S. Typhimurium resulted in three folds more invasion in chicken monocyte derived macrophages.
29395289	7	72	theme	carbohydrate	931:942	arg1	receptors					944:952	carbohydrate receptors	931:952	carbohydrate receptors of macrophages	931:967	Next we attempted to analyse the role of carbohydrate receptors of macrophages in S. Typhimurium invasion.
29395289	2	73	theme	cell	288:291	arg1	surface					293:299	the cell surface	284:299	the cell surface of host	284:307	The attachment is mediated through membrane expressed adhesins present on the organism and receptors on the cell surface of host.
29395289	9	74	theme	Typhimurium	1276:1286	arg1	invasion					1288:1295	S. Typhimurium invasion	1273:1295	S. Typhimurium invasion	1273:1295	Our data suggest that deglycosylation of N-linked glycans including sialic acid by PNGase F is more effective in inhibition of S. Typhimurium invasion than neuraminidase which removes only sialic acid.
29395289	3	75	theme	study	332:336	arg1	objective					314:322	The objective	310:322	The objective of this study	310:336	The objective of this study was to investigate the significance of Fc receptors (FcRs), actin filament polymerization, mannose receptors (MRs), carbohydrate moieties like N-linked glycans and sialic acid on chicken macrophages for invasion of S. Typhimurium.
29395289	3	76	theme	filament	404:411	arg1	polymerization					413:426	actin filament polymerization	398:426	actin filament polymerization	398:426	The objective of this study was to investigate the significance of Fc receptors (FcRs), actin filament polymerization, mannose receptors (MRs), carbohydrate moieties like N-linked glycans and sialic acid on chicken macrophages for invasion of S. Typhimurium.
29395289	8	77	theme	PNGase	1055:1060	arg1	F					1062:1062	PNGase F	1055:1062	PNGase F	1055:1062	Treatment of macrophages with methyl α-d-mannopyranoside, PNGase F and neuraminidase, showed 2.5, 5 and 2.5 folds decrease in invasion respectively.
29395289	0	78	theme	receptors	18:26	arg1	Role					0:3	Role	0:3	Role of different receptors and actin filaments on Salmonella Typhimurium invasion in chicken macrophages.	0:105	Role of different receptors and actin filaments on Salmonella Typhimurium invasion in chicken macrophages.
29395289	3	79	theme	receptors	437:445	arg1	significance					361:372	the significance	357:372	the significance of Fc receptors (FcRs), actin filament polymerization, mannose receptors (MRs), carbohydrate moieties like N-linked glycans and sialic acid on chicken macrophages for invasion of S. Typhimurium	357:566	The objective of this study was to investigate the significance of Fc receptors (FcRs), actin filament polymerization, mannose receptors (MRs), carbohydrate moieties like N-linked glycans and sialic acid on chicken macrophages for invasion of S. Typhimurium.
29395289	0	80	theme	actin	32:36	arg1	filaments					38:46	actin filaments	32:46	actin filaments	32:46	Role of different receptors and actin filaments on Salmonella Typhimurium invasion in chicken macrophages.
29395289	4	81	dep	three	612:616	arg1	folds					618:622	folds	618:622	folds more invasion in chicken monocyte derived macrophages	618:676	Opsonisation of S. Typhimurium resulted in three folds more invasion in chicken monocyte derived macrophages.
29395289	6	82	dep	decrease	842:849	arg1	28					852:853	28	852:853	28	852:853	Pre-incubation of macrophages with cytochalasin D, showed severe decrease (28 folds) in S. Typhimurium invasion.
29395289	3	83	from	polymerization	413:426	arg1	macrophages					525:535	chicken macrophages	517:535	chicken macrophages	517:535	The objective of this study was to investigate the significance of Fc receptors (FcRs), actin filament polymerization, mannose receptors (MRs), carbohydrate moieties like N-linked glycans and sialic acid on chicken macrophages for invasion of S. Typhimurium.
29395289	5	84	theme	Typhimurium	764:774	arg1	uptake					751:756	uptake	751:756	uptake of S. Typhimurium	751:774	Cytochalasin D, an inhibitor of actin filament polymerization prevented uptake of S. Typhimurium.
29395289	6	85	theme	severe	835:840	arg1	decrease					842:849	severe decrease	835:849	severe decrease (28 folds)	835:860	Pre-incubation of macrophages with cytochalasin D, showed severe decrease (28 folds) in S. Typhimurium invasion.
29395289	9	86	theme	invasion	1288:1295	arg1	inhibition					1259:1268	inhibition	1259:1268	inhibition of S. Typhimurium invasion	1259:1295	Our data suggest that deglycosylation of N-linked glycans including sialic acid by PNGase F is more effective in inhibition of S. Typhimurium invasion than neuraminidase which removes only sialic acid.
29395289	3	87	theme	receptors	380:388	arg1	significance					361:372	the significance	357:372	the significance of Fc receptors (FcRs), actin filament polymerization, mannose receptors (MRs), carbohydrate moieties like N-linked glycans and sialic acid on chicken macrophages for invasion of S. Typhimurium	357:566	The objective of this study was to investigate the significance of Fc receptors (FcRs), actin filament polymerization, mannose receptors (MRs), carbohydrate moieties like N-linked glycans and sialic acid on chicken macrophages for invasion of S. Typhimurium.
29395289	6	88	dep	28	852:853	arg1	folds					855:859	folds	855:859	folds	855:859	Pre-incubation of macrophages with cytochalasin D, showed severe decrease (28 folds) in S. Typhimurium invasion.
29395289	3	89	theme	Typhimurium	556:566	arg1	invasion					541:548	invasion	541:548	invasion of S. Typhimurium	541:566	The objective of this study was to investigate the significance of Fc receptors (FcRs), actin filament polymerization, mannose receptors (MRs), carbohydrate moieties like N-linked glycans and sialic acid on chicken macrophages for invasion of S. Typhimurium.
29395289	9	90	link	N-linked	1187:1194	arg1	acid					1221:1224	sialic acid	1214:1224	sialic acid	1214:1224	Our data suggest that deglycosylation of N-linked glycans including sialic acid by PNGase F is more effective in inhibition of S. Typhimurium invasion than neuraminidase which removes only sialic acid.
29395289	9	90	link	N-linked	1187:1194	arg1	glycans					1196:1202	N-linked glycans	1187:1202	N-linked glycans including sialic acid	1187:1224	Our data suggest that deglycosylation of N-linked glycans including sialic acid by PNGase F is more effective in inhibition of S. Typhimurium invasion than neuraminidase which removes only sialic acid.
29395289	6	91	theme	cytochalasin	812:823	arg1	D					825:825	cytochalasin D	812:825	cytochalasin D	812:825	Pre-incubation of macrophages with cytochalasin D, showed severe decrease (28 folds) in S. Typhimurium invasion.
29395289	3	92	theme	N-linked	481:488	arg1	glycans					490:496	N-linked glycans	481:496	N-linked glycans	481:496	The objective of this study was to investigate the significance of Fc receptors (FcRs), actin filament polymerization, mannose receptors (MRs), carbohydrate moieties like N-linked glycans and sialic acid on chicken macrophages for invasion of S. Typhimurium.
29395289	10	93	dep	suggested	1363:1371	arg1	act					1452:1454	act	1452:1454	suggested FcRs, actin filament polymerization, MRs, N-linked glycans and sialic acid may act as gateway for entry of S. Typhimurium	1363:1493	These findings suggested FcRs, actin filament polymerization, MRs, N-linked glycans and sialic acid may act as gateway for entry of S. Typhimurium.
31778109	4	0	theme	treatment	593:601	arg1	interventions					603:615	treatment interventions	593:615	treatment interventions	593:615	We aimed to determine the differences in intestinal microbial composition between healthy individuals and patients with AS who received and who did not receive treatment interventions.
31778109	10	1	theme	Clostridiales	1792:1804	arg1	1_7_47FAA					1816:1824	Clostridiales bacterium 1_7_47FAA	1792:1824	Clostridiales bacterium 1_7_47FAA	1792:1824	F. plautii was found to be significantly increased in the comparison between the HC and WM groups, along with four other species (Clostridium bolteae, Clostridiales bacterium 1_7_47FAA, C. asparagiforme and C. hathewayi).
31778109	6	2	theme	drug	1130:1133	arg1	analysis					1142:1149	the drug effect analysis	1126:1149	the drug effect analysis	1126:1149	Sixty-six faecal DNA samples, including 37 from healthy controls (HCs), 11 from patients with untreated AS (NM), 7 from patients treated with nonsteroidal anti-inflammatory drugs (e.g. celecoxib; WM) and 11 from patients treated with Chinese herbal medicine (CHM), such as the Bushen-Qiangdu-Zhilv decoction, were collected and used in the drug effect analysis.
31778109	2	3	dep	spondylitis	206:216	arg1	AS					219:220	AS	219:220	AS	219:220	Ankylosing spondylitis (AS) is a systemic progressive disease with an unknown etiology that may be related to the gut microbiome.
31778109	13	4	with	microbiota	2124:2133	arg1	AS					2156:2157	AS	2156:2157	AS	2156:2157	The gut microbiota of the patients with AS varied from that of the HCs, and the treatment had an impact on this divergence.
31778109	14	5	from	improvements	2282:2293	arg1	treatment					2301:2309	AS treatment	2298:2309	AS treatment	2298:2309	Our data provide insight that could guide improvements in AS treatment.
31778109	12	6	theme	able	2036:2039	arg1	profiles					2022:2029	bacterial profiles	2012:2029	bacterial profiles less able to biodegrade xenobiotics or synthesize and transport vitamins.Conclusion	2012:2113	They also had bacterial profiles less able to biodegrade xenobiotics or synthesize and transport vitamins.Conclusion.
31778109	11	7	theme	more	1894:1897	arg1	species					1909:1915	more bacterial species	1894:1915	more bacterial species associated with carbohydrate metabolism and glycan biosynthesis in their faeces	1894:1995	The patients with AS harboured more bacterial species associated with carbohydrate metabolism and glycan biosynthesis in their faeces.
31778109	9	8	theme	CHM	1600:1602	arg1	effect					1590:1595	a beneficial effect	1577:1595	a beneficial effect of CHM	1577:1602	No additional species were found in the HC vs. CHM analysis, which indicated a beneficial effect of CHM in removing the other three strains.
31778109	13	9	theme	patients	2142:2149	arg1	microbiota					2124:2133	The gut microbiota	2116:2133	The gut microbiota of the patients with AS	2116:2157	The gut microbiota of the patients with AS varied from that of the HCs, and the treatment had an impact on this divergence.
31778109	12	10	theme	biodegrade	2044:2053	arg1	xenobiotics					2055:2065	biodegrade xenobiotics	2044:2065	biodegrade xenobiotics or synthesize and transport vitamins.Conclusion	2044:2113	They also had bacterial profiles less able to biodegrade xenobiotics or synthesize and transport vitamins.Conclusion.
31778109	6	11	theme	faecal	800:805	arg1	samples					811:817	Sixty-six faecal DNA samples	790:817	Sixty-six faecal DNA samples	790:817	Sixty-six faecal DNA samples, including 37 from healthy controls (HCs), 11 from patients with untreated AS (NM), 7 from patients treated with nonsteroidal anti-inflammatory drugs (e.g. celecoxib; WM) and 11 from patients treated with Chinese herbal medicine (CHM), such as the Bushen-Qiangdu-Zhilv decoction, were collected and used in the drug effect analysis.
31778109	6	11	theme	faecal	800:805	arg1	decoction					1088:1096	the Bushen-Qiangdu-Zhilv decoction	1063:1096	the Bushen-Qiangdu-Zhilv decoction	1063:1096	Sixty-six faecal DNA samples, including 37 from healthy controls (HCs), 11 from patients with untreated AS (NM), 7 from patients treated with nonsteroidal anti-inflammatory drugs (e.g. celecoxib; WM) and 11 from patients treated with Chinese herbal medicine (CHM), such as the Bushen-Qiangdu-Zhilv decoction, were collected and used in the drug effect analysis.
31778109	6	11	theme	faecal	800:805	arg1	7					903:903	7	903:903	7	903:903	Sixty-six faecal DNA samples, including 37 from healthy controls (HCs), 11 from patients with untreated AS (NM), 7 from patients treated with nonsteroidal anti-inflammatory drugs (e.g. celecoxib; WM) and 11 from patients treated with Chinese herbal medicine (CHM), such as the Bushen-Qiangdu-Zhilv decoction, were collected and used in the drug effect analysis.
31778109	6	11	theme	faecal	800:805	arg1	37					830:831	37	830:831	37	830:831	Sixty-six faecal DNA samples, including 37 from healthy controls (HCs), 11 from patients with untreated AS (NM), 7 from patients treated with nonsteroidal anti-inflammatory drugs (e.g. celecoxib; WM) and 11 from patients treated with Chinese herbal medicine (CHM), such as the Bushen-Qiangdu-Zhilv decoction, were collected and used in the drug effect analysis.
31778109	6	11	theme	faecal	800:805	arg1	11					994:995	11	994:995	11	994:995	Sixty-six faecal DNA samples, including 37 from healthy controls (HCs), 11 from patients with untreated AS (NM), 7 from patients treated with nonsteroidal anti-inflammatory drugs (e.g. celecoxib; WM) and 11 from patients treated with Chinese herbal medicine (CHM), such as the Bushen-Qiangdu-Zhilv decoction, were collected and used in the drug effect analysis.
31778109	6	11	theme	faecal	800:805	arg1	11					862:863	11	862:863	11	862:863	Sixty-six faecal DNA samples, including 37 from healthy controls (HCs), 11 from patients with untreated AS (NM), 7 from patients treated with nonsteroidal anti-inflammatory drugs (e.g. celecoxib; WM) and 11 from patients treated with Chinese herbal medicine (CHM), such as the Bushen-Qiangdu-Zhilv decoction, were collected and used in the drug effect analysis.
31778109	2	12	theme	gut	309:311	arg1	microbiome					313:322	the gut microbiome	305:322	the gut microbiome	305:322	Ankylosing spondylitis (AS) is a systemic progressive disease with an unknown etiology that may be related to the gut microbiome.
31778109	6	13	used	used	1118:1121	arg2	11					994:995	11	994:995	11	994:995	Sixty-six faecal DNA samples, including 37 from healthy controls (HCs), 11 from patients with untreated AS (NM), 7 from patients treated with nonsteroidal anti-inflammatory drugs (e.g. celecoxib; WM) and 11 from patients treated with Chinese herbal medicine (CHM), such as the Bushen-Qiangdu-Zhilv decoction, were collected and used in the drug effect analysis.
31778109	6	13	used	used	1118:1121	arg2	37					830:831	37	830:831	37	830:831	Sixty-six faecal DNA samples, including 37 from healthy controls (HCs), 11 from patients with untreated AS (NM), 7 from patients treated with nonsteroidal anti-inflammatory drugs (e.g. celecoxib; WM) and 11 from patients treated with Chinese herbal medicine (CHM), such as the Bushen-Qiangdu-Zhilv decoction, were collected and used in the drug effect analysis.
31778109	6	13	used	used	1118:1121	arg2	7					903:903	7	903:903	7	903:903	Sixty-six faecal DNA samples, including 37 from healthy controls (HCs), 11 from patients with untreated AS (NM), 7 from patients treated with nonsteroidal anti-inflammatory drugs (e.g. celecoxib; WM) and 11 from patients treated with Chinese herbal medicine (CHM), such as the Bushen-Qiangdu-Zhilv decoction, were collected and used in the drug effect analysis.
31778109	6	13	used	used	1118:1121	arg2	11					862:863	11	862:863	11	862:863	Sixty-six faecal DNA samples, including 37 from healthy controls (HCs), 11 from patients with untreated AS (NM), 7 from patients treated with nonsteroidal anti-inflammatory drugs (e.g. celecoxib; WM) and 11 from patients treated with Chinese herbal medicine (CHM), such as the Bushen-Qiangdu-Zhilv decoction, were collected and used in the drug effect analysis.
31778109	6	13	used	used	1118:1121	arg2	decoction					1088:1096	the Bushen-Qiangdu-Zhilv decoction	1063:1096	the Bushen-Qiangdu-Zhilv decoction	1063:1096	Sixty-six faecal DNA samples, including 37 from healthy controls (HCs), 11 from patients with untreated AS (NM), 7 from patients treated with nonsteroidal anti-inflammatory drugs (e.g. celecoxib; WM) and 11 from patients treated with Chinese herbal medicine (CHM), such as the Bushen-Qiangdu-Zhilv decoction, were collected and used in the drug effect analysis.
31778109	6	13	used	used	1118:1121	arg2	samples					811:817	Sixty-six faecal DNA samples	790:817	Sixty-six faecal DNA samples	790:817	Sixty-six faecal DNA samples, including 37 from healthy controls (HCs), 11 from patients with untreated AS (NM), 7 from patients treated with nonsteroidal anti-inflammatory drugs (e.g. celecoxib; WM) and 11 from patients treated with Chinese herbal medicine (CHM), such as the Bushen-Qiangdu-Zhilv decoction, were collected and used in the drug effect analysis.
31778109	12	14	dep	xenobiotics	2055:2065	arg1	synthesize					2070:2079	synthesize	2070:2079	synthesize	2070:2079	They also had bacterial profiles less able to biodegrade xenobiotics or synthesize and transport vitamins.Conclusion.
31778109	12	14	dep	xenobiotics	2055:2065	arg1	transport					2085:2093	transport	2085:2093	transport vitamins.Conclusion	2085:2113	They also had bacterial profiles less able to biodegrade xenobiotics or synthesize and transport vitamins.Conclusion.
31778109	6	15	theme	anti-inflammatory	945:961	arg1	celecoxib					975:983	e.g. celecoxib	970:983	e.g. celecoxib	970:983	Sixty-six faecal DNA samples, including 37 from healthy controls (HCs), 11 from patients with untreated AS (NM), 7 from patients treated with nonsteroidal anti-inflammatory drugs (e.g. celecoxib; WM) and 11 from patients treated with Chinese herbal medicine (CHM), such as the Bushen-Qiangdu-Zhilv decoction, were collected and used in the drug effect analysis.
31778109	6	15	theme	anti-inflammatory	945:961	arg1	drugs					963:967	nonsteroidal anti-inflammatory drugs	932:967	nonsteroidal anti-inflammatory drugs (e.g. celecoxib; WM)	932:988	Sixty-six faecal DNA samples, including 37 from healthy controls (HCs), 11 from patients with untreated AS (NM), 7 from patients treated with nonsteroidal anti-inflammatory drugs (e.g. celecoxib; WM) and 11 from patients treated with Chinese herbal medicine (CHM), such as the Bushen-Qiangdu-Zhilv decoction, were collected and used in the drug effect analysis.
31778109	4	16	from	differences	459:469	arg1	composition					495:505	intestinal microbial composition	474:505	intestinal microbial composition	474:505	We aimed to determine the differences in intestinal microbial composition between healthy individuals and patients with AS who received and who did not receive treatment interventions.
31778109	9	17	theme	other	1620:1624	arg1	strains					1632:1638	the other three strains	1616:1638	the other three strains	1616:1638	No additional species were found in the HC vs. CHM analysis, which indicated a beneficial effect of CHM in removing the other three strains.
31778109	0	18	from	composition	82:92	arg1	study					28:32	Metagenome-wide association study	0:32	Metagenome-wide association study of the alterations in the intestinal microbiome composition of ankylosing spondylitis patients and the effect of traditional and herbal treatment.	0:179	Metagenome-wide association study of the alterations in the intestinal microbiome composition of ankylosing spondylitis patients and the effect of traditional and herbal treatment.
31778109	2	19	theme	progressive	237:247	arg1	disease					249:255	a systemic progressive disease	226:255	a systemic progressive disease with an unknown etiology that may be related to the gut microbiome	226:322	Ankylosing spondylitis (AS) is a systemic progressive disease with an unknown etiology that may be related to the gut microbiome.
31778109	2	19	theme	progressive	237:247	arg1	spondylitis					206:216	Ankylosing spondylitis	195:216	Ankylosing spondylitis (AS)	195:221	Ankylosing spondylitis (AS) is a systemic progressive disease with an unknown etiology that may be related to the gut microbiome.
31778109	8	20	dep	patients	1293:1300	arg1	distasonis					1366:1375	distasonis	1366:1375	distasonis	1366:1375	Four species were enriched in the patients with AS: Flavonifractor plautii, Oscillibacter, Parabacteroides distasonis and Bacteroides nordii (HC vs. NM, P<0.05); only F. plautii was found to be significantly changed in the NM-HC comparison.
31778109	8	20	dep	patients	1293:1300	arg1	nordii					1393:1398	Bacteroides nordii	1381:1398	Bacteroides nordii (HC vs. NM, P<0.05)	1381:1418	Four species were enriched in the patients with AS: Flavonifractor plautii, Oscillibacter, Parabacteroides distasonis and Bacteroides nordii (HC vs. NM, P<0.05); only F. plautii was found to be significantly changed in the NM-HC comparison.
31778109	8	20	dep	patients	1293:1300	arg1	patients					1293:1300	the patients	1289:1300	the patients with AS: Flavonifractor plautii, Oscillibacter, Parabacteroides distasonis and Bacteroides nordii (HC vs. NM, P<0.05)	1289:1418	Four species were enriched in the patients with AS: Flavonifractor plautii, Oscillibacter, Parabacteroides distasonis and Bacteroides nordii (HC vs. NM, P<0.05); only F. plautii was found to be significantly changed in the NM-HC comparison.
31778109	8	20	dep	patients	1293:1300	arg1	Parabacteroides					1350:1364	Parabacteroides	1350:1364	Parabacteroides	1350:1364	Four species were enriched in the patients with AS: Flavonifractor plautii, Oscillibacter, Parabacteroides distasonis and Bacteroides nordii (HC vs. NM, P<0.05); only F. plautii was found to be significantly changed in the NM-HC comparison.
31778109	8	20	dep	patients	1293:1300	arg1	Flavonifractor					1311:1324	Flavonifractor	1311:1324	Flavonifractor	1311:1324	Four species were enriched in the patients with AS: Flavonifractor plautii, Oscillibacter, Parabacteroides distasonis and Bacteroides nordii (HC vs. NM, P<0.05); only F. plautii was found to be significantly changed in the NM-HC comparison.
31778109	0	21	theme	treatment	170:178	arg1	alterations					41:51	the alterations	37:51	the alterations in the intestinal microbiome composition of ankylosing spondylitis patients	37:127	Metagenome-wide association study of the alterations in the intestinal microbiome composition of ankylosing spondylitis patients and the effect of traditional and herbal treatment.
31778109	0	21	theme	treatment	170:178	arg1	effect					137:142	the effect	133:142	the effect of traditional and herbal treatment	133:178	Metagenome-wide association study of the alterations in the intestinal microbiome composition of ankylosing spondylitis patients and the effect of traditional and herbal treatment.
31778109	2	22	theme	unknown	265:271	arg1	related					294:300	related	294:300	related	294:300	Ankylosing spondylitis (AS) is a systemic progressive disease with an unknown etiology that may be related to the gut microbiome.
31778109	2	22	theme	unknown	265:271	arg1	etiology					273:280	an unknown etiology	262:280	an unknown etiology that may be related to the gut microbiome	262:322	Ankylosing spondylitis (AS) is a systemic progressive disease with an unknown etiology that may be related to the gut microbiome.
31778109	11	23	with	patients	1867:1874	arg1	AS					1881:1882	AS	1881:1882	AS	1881:1882	The patients with AS harboured more bacterial species associated with carbohydrate metabolism and glycan biosynthesis in their faeces.
31778109	0	24	from	study	28:32	arg1	composition					82:92	the intestinal microbiome composition	56:92	the intestinal microbiome composition of ankylosing spondylitis patients	56:127	Metagenome-wide association study of the alterations in the intestinal microbiome composition of ankylosing spondylitis patients and the effect of traditional and herbal treatment.
31778109	9	25	theme	beneficial	1579:1588	arg1	effect					1590:1595	a beneficial effect	1577:1595	a beneficial effect of CHM	1577:1602	No additional species were found in the HC vs. CHM analysis, which indicated a beneficial effect of CHM in removing the other three strains.
31778109	6	26	dep	celecoxib	975:983	arg1	WM					986:987	WM	986:987	WM	986:987	Sixty-six faecal DNA samples, including 37 from healthy controls (HCs), 11 from patients with untreated AS (NM), 7 from patients treated with nonsteroidal anti-inflammatory drugs (e.g. celecoxib; WM) and 11 from patients treated with Chinese herbal medicine (CHM), such as the Bushen-Qiangdu-Zhilv decoction, were collected and used in the drug effect analysis.
31778109	0	27	theme	traditional	147:157	arg1	treatment					170:178	traditional and herbal treatment	147:178	traditional and herbal treatment	147:178	Metagenome-wide association study of the alterations in the intestinal microbiome composition of ankylosing spondylitis patients and the effect of traditional and herbal treatment.
31778109	11	28	from	biosynthesis	1968:1979	arg1	faeces					1990:1995	their faeces	1984:1995	their faeces	1984:1995	The patients with AS harboured more bacterial species associated with carbohydrate metabolism and glycan biosynthesis in their faeces.
31778109	0	29	theme	ankylosing	97:106	arg1	spondylitis					108:118	ankylosing spondylitis	97:118	ankylosing spondylitis patients	97:127	Metagenome-wide association study of the alterations in the intestinal microbiome composition of ankylosing spondylitis patients and the effect of traditional and herbal treatment.
31778109	4	30	theme	intestinal	474:483	arg1	composition					495:505	intestinal microbial composition	474:505	intestinal microbial composition	474:505	We aimed to determine the differences in intestinal microbial composition between healthy individuals and patients with AS who received and who did not receive treatment interventions.
31778109	0	31	theme	patients	120:127	arg1	composition					82:92	the intestinal microbiome composition	56:92	the intestinal microbiome composition of ankylosing spondylitis patients	56:127	Metagenome-wide association study of the alterations in the intestinal microbiome composition of ankylosing spondylitis patients and the effect of traditional and herbal treatment.
31778109	0	32	theme	Metagenome-wide	0:14	arg1	association					16:26	Metagenome-wide association	0:26	Metagenome-wide association study of the alterations in the intestinal microbiome composition of ankylosing spondylitis patients and the effect of traditional and herbal treatment.	0:179	Metagenome-wide association study of the alterations in the intestinal microbiome composition of ankylosing spondylitis patients and the effect of traditional and herbal treatment.
31778109	8	33	dep	F.	1426:1427	arg1	plautii					1429:1435	F. plautii	1426:1435	F. plautii	1426:1435	Four species were enriched in the patients with AS: Flavonifractor plautii, Oscillibacter, Parabacteroides distasonis and Bacteroides nordii (HC vs. NM, P<0.05); only F. plautii was found to be significantly changed in the NM-HC comparison.
31778109	9	34	theme	additional	1503:1512	arg1	species					1514:1520	No additional species	1500:1520	No additional species	1500:1520	No additional species were found in the HC vs. CHM analysis, which indicated a beneficial effect of CHM in removing the other three strains.
31778109	6	35	theme	Chinese	1024:1030	arg1	CHM					1049:1051	CHM	1049:1051	CHM	1049:1051	Sixty-six faecal DNA samples, including 37 from healthy controls (HCs), 11 from patients with untreated AS (NM), 7 from patients treated with nonsteroidal anti-inflammatory drugs (e.g. celecoxib; WM) and 11 from patients treated with Chinese herbal medicine (CHM), such as the Bushen-Qiangdu-Zhilv decoction, were collected and used in the drug effect analysis.
31778109	6	35	theme	Chinese	1024:1030	arg1	medicine					1039:1046	Chinese herbal medicine	1024:1046	Chinese herbal medicine (CHM)	1024:1052	Sixty-six faecal DNA samples, including 37 from healthy controls (HCs), 11 from patients with untreated AS (NM), 7 from patients treated with nonsteroidal anti-inflammatory drugs (e.g. celecoxib; WM) and 11 from patients treated with Chinese herbal medicine (CHM), such as the Bushen-Qiangdu-Zhilv decoction, were collected and used in the drug effect analysis.
31778109	5	36	from	pathology	635:643	arg1	patient					659:665	each patient	654:665	each patient	654:665	In parallel, the pathology of AS in each patient was analysed to better understand the link between AS treatment and the intestinal microbiota of the patients.Methodology.
31778109	3	37	theme	future	413:418	arg1	therapy.Aim					420:430	future therapy.Aim	413:430	future therapy.Aim	413:430	Therefore, a more thorough understanding of its pathogenesis is necessary for directing future therapy.Aim.
31778109	2	38	theme	Ankylosing	195:204	arg1	spondylitis					206:216	Ankylosing spondylitis	195:216	Ankylosing spondylitis (AS)	195:221	Ankylosing spondylitis (AS) is a systemic progressive disease with an unknown etiology that may be related to the gut microbiome.
31778109	2	38	theme	Ankylosing	195:204	arg1	disease					249:255	a systemic progressive disease	226:255	a systemic progressive disease with an unknown etiology that may be related to the gut microbiome	226:322	Ankylosing spondylitis (AS) is a systemic progressive disease with an unknown etiology that may be related to the gut microbiome.
31778109	13	39	theme	gut	2120:2122	arg1	microbiota					2124:2133	The gut microbiota	2116:2133	The gut microbiota of the patients with AS	2116:2157	The gut microbiota of the patients with AS varied from that of the HCs, and the treatment had an impact on this divergence.
31778109	0	40	theme	intestinal	60:69	arg1	composition					82:92	the intestinal microbiome composition	56:92	the intestinal microbiome composition of ankylosing spondylitis patients	56:127	Metagenome-wide association study of the alterations in the intestinal microbiome composition of ankylosing spondylitis patients and the effect of traditional and herbal treatment.
31778109	13	41	from	impact	2213:2218	arg1	divergence					2228:2237	this divergence	2223:2237	this divergence	2223:2237	The gut microbiota of the patients with AS varied from that of the HCs, and the treatment had an impact on this divergence.
31778109	4	42	with	patients	539:546	arg1	AS					553:554	AS	553:554	AS	553:554	We aimed to determine the differences in intestinal microbial composition between healthy individuals and patients with AS who received and who did not receive treatment interventions.
31778109	9	43	theme	HC	1540:1541	arg1	analysis					1551:1558	the HC vs. CHM analysis	1536:1558	the HC vs. CHM analysis	1536:1558	No additional species were found in the HC vs. CHM analysis, which indicated a beneficial effect of CHM in removing the other three strains.
31778109	6	44	theme	Bushen-Qiangdu-Zhilv	1067:1086	arg1	decoction					1088:1096	the Bushen-Qiangdu-Zhilv decoction	1063:1096	the Bushen-Qiangdu-Zhilv decoction	1063:1096	Sixty-six faecal DNA samples, including 37 from healthy controls (HCs), 11 from patients with untreated AS (NM), 7 from patients treated with nonsteroidal anti-inflammatory drugs (e.g. celecoxib; WM) and 11 from patients treated with Chinese herbal medicine (CHM), such as the Bushen-Qiangdu-Zhilv decoction, were collected and used in the drug effect analysis.
31778109	4	45	with	individuals	523:533	arg1	AS					553:554	AS	553:554	AS	553:554	We aimed to determine the differences in intestinal microbial composition between healthy individuals and patients with AS who received and who did not receive treatment interventions.
31778109	9	46	theme	CHM	1547:1549	arg1	analysis					1551:1558	the HC vs. CHM analysis	1536:1558	the HC vs. CHM analysis	1536:1558	No additional species were found in the HC vs. CHM analysis, which indicated a beneficial effect of CHM in removing the other three strains.
31778109	7	47	theme	microbial	1213:1221	arg1	determined.Results					1239:1256	determined.Results	1239:1256	determined.Results	1239:1256	All samples were sequenced using Illumina HiSeq 4000 and the microbial composition was determined.Results.
31778109	7	47	theme	microbial	1213:1221	arg1	composition					1223:1233	the microbial composition	1209:1233	the microbial composition	1209:1233	All samples were sequenced using Illumina HiSeq 4000 and the microbial composition was determined.Results.
31778109	10	48	dep	species	1762:1768	arg1	1_7_47FAA					1816:1824	Clostridiales bacterium 1_7_47FAA	1792:1824	Clostridiales bacterium 1_7_47FAA	1792:1824	F. plautii was found to be significantly increased in the comparison between the HC and WM groups, along with four other species (Clostridium bolteae, Clostridiales bacterium 1_7_47FAA, C. asparagiforme and C. hathewayi).
31778109	10	48	dep	species	1762:1768	arg1	asparagiforme					1830:1842	C. asparagiforme	1827:1842	C. asparagiforme	1827:1842	F. plautii was found to be significantly increased in the comparison between the HC and WM groups, along with four other species (Clostridium bolteae, Clostridiales bacterium 1_7_47FAA, C. asparagiforme and C. hathewayi).
31778109	10	48	dep	species	1762:1768	arg1	hathewayi					1851:1859	C. hathewayi	1848:1859	C. hathewayi	1848:1859	F. plautii was found to be significantly increased in the comparison between the HC and WM groups, along with four other species (Clostridium bolteae, Clostridiales bacterium 1_7_47FAA, C. asparagiforme and C. hathewayi).
31778109	10	48	dep	species	1762:1768	arg1	bolteae					1783:1789	Clostridium bolteae	1771:1789	Clostridium bolteae	1771:1789	F. plautii was found to be significantly increased in the comparison between the HC and WM groups, along with four other species (Clostridium bolteae, Clostridiales bacterium 1_7_47FAA, C. asparagiforme and C. hathewayi).
31778109	6	49	with	patients	870:877	arg1	untreated					884:892	untreated	884:892	untreated	884:892	Sixty-six faecal DNA samples, including 37 from healthy controls (HCs), 11 from patients with untreated AS (NM), 7 from patients treated with nonsteroidal anti-inflammatory drugs (e.g. celecoxib; WM) and 11 from patients treated with Chinese herbal medicine (CHM), such as the Bushen-Qiangdu-Zhilv decoction, were collected and used in the drug effect analysis.
31778109	6	49	with	patients	870:877	arg1	NM					898:899	NM	898:899	NM	898:899	Sixty-six faecal DNA samples, including 37 from healthy controls (HCs), 11 from patients with untreated AS (NM), 7 from patients treated with nonsteroidal anti-inflammatory drugs (e.g. celecoxib; WM) and 11 from patients treated with Chinese herbal medicine (CHM), such as the Bushen-Qiangdu-Zhilv decoction, were collected and used in the drug effect analysis.
31778109	10	50	theme	bacterium	1806:1814	arg1	1_7_47FAA					1816:1824	Clostridiales bacterium 1_7_47FAA	1792:1824	Clostridiales bacterium 1_7_47FAA	1792:1824	F. plautii was found to be significantly increased in the comparison between the HC and WM groups, along with four other species (Clostridium bolteae, Clostridiales bacterium 1_7_47FAA, C. asparagiforme and C. hathewayi).
31778109	4	51	theme	healthy	515:521	arg1	individuals					523:533	healthy individuals	515:533	healthy individuals	515:533	We aimed to determine the differences in intestinal microbial composition between healthy individuals and patients with AS who received and who did not receive treatment interventions.
31778109	11	52	theme	bacterial	1899:1907	arg1	species					1909:1915	more bacterial species	1894:1915	more bacterial species associated with carbohydrate metabolism and glycan biosynthesis in their faeces	1894:1995	The patients with AS harboured more bacterial species associated with carbohydrate metabolism and glycan biosynthesis in their faeces.
31778109	12	53	contain	had	2008:2010	arg2	profiles					2022:2029	bacterial profiles	2012:2029	bacterial profiles less able to biodegrade xenobiotics or synthesize and transport vitamins.Conclusion	2012:2113	They also had bacterial profiles less able to biodegrade xenobiotics or synthesize and transport vitamins.Conclusion.
31778109	12	53	contain	had	2008:2010	arg1	They					1998:2001	They	1998:2001	They	1998:2001	They also had bacterial profiles less able to biodegrade xenobiotics or synthesize and transport vitamins.Conclusion.
31778109	5	54	theme	AS	648:649	arg1	pathology					635:643	the pathology	631:643	the pathology of AS in each patient	631:665	In parallel, the pathology of AS in each patient was analysed to better understand the link between AS treatment and the intestinal microbiota of the patients.Methodology.
31778109	6	55	theme	DNA	807:809	arg1	samples					811:817	Sixty-six faecal DNA samples	790:817	Sixty-six faecal DNA samples	790:817	Sixty-six faecal DNA samples, including 37 from healthy controls (HCs), 11 from patients with untreated AS (NM), 7 from patients treated with nonsteroidal anti-inflammatory drugs (e.g. celecoxib; WM) and 11 from patients treated with Chinese herbal medicine (CHM), such as the Bushen-Qiangdu-Zhilv decoction, were collected and used in the drug effect analysis.
31778109	6	55	theme	DNA	807:809	arg1	decoction					1088:1096	the Bushen-Qiangdu-Zhilv decoction	1063:1096	the Bushen-Qiangdu-Zhilv decoction	1063:1096	Sixty-six faecal DNA samples, including 37 from healthy controls (HCs), 11 from patients with untreated AS (NM), 7 from patients treated with nonsteroidal anti-inflammatory drugs (e.g. celecoxib; WM) and 11 from patients treated with Chinese herbal medicine (CHM), such as the Bushen-Qiangdu-Zhilv decoction, were collected and used in the drug effect analysis.
31778109	6	55	theme	DNA	807:809	arg1	7					903:903	7	903:903	7	903:903	Sixty-six faecal DNA samples, including 37 from healthy controls (HCs), 11 from patients with untreated AS (NM), 7 from patients treated with nonsteroidal anti-inflammatory drugs (e.g. celecoxib; WM) and 11 from patients treated with Chinese herbal medicine (CHM), such as the Bushen-Qiangdu-Zhilv decoction, were collected and used in the drug effect analysis.
31778109	6	55	theme	DNA	807:809	arg1	37					830:831	37	830:831	37	830:831	Sixty-six faecal DNA samples, including 37 from healthy controls (HCs), 11 from patients with untreated AS (NM), 7 from patients treated with nonsteroidal anti-inflammatory drugs (e.g. celecoxib; WM) and 11 from patients treated with Chinese herbal medicine (CHM), such as the Bushen-Qiangdu-Zhilv decoction, were collected and used in the drug effect analysis.
31778109	6	55	theme	DNA	807:809	arg1	11					994:995	11	994:995	11	994:995	Sixty-six faecal DNA samples, including 37 from healthy controls (HCs), 11 from patients with untreated AS (NM), 7 from patients treated with nonsteroidal anti-inflammatory drugs (e.g. celecoxib; WM) and 11 from patients treated with Chinese herbal medicine (CHM), such as the Bushen-Qiangdu-Zhilv decoction, were collected and used in the drug effect analysis.
31778109	6	55	theme	DNA	807:809	arg1	11					862:863	11	862:863	11	862:863	Sixty-six faecal DNA samples, including 37 from healthy controls (HCs), 11 from patients with untreated AS (NM), 7 from patients treated with nonsteroidal anti-inflammatory drugs (e.g. celecoxib; WM) and 11 from patients treated with Chinese herbal medicine (CHM), such as the Bushen-Qiangdu-Zhilv decoction, were collected and used in the drug effect analysis.
31778109	11	56	theme	carbohydrate	1933:1944	arg1	metabolism					1946:1955	carbohydrate metabolism	1933:1955	carbohydrate metabolism	1933:1955	The patients with AS harboured more bacterial species associated with carbohydrate metabolism and glycan biosynthesis in their faeces.
31778109	0	57	theme	effect	137:142	arg1	study					28:32	Metagenome-wide association study	0:32	Metagenome-wide association study of the alterations in the intestinal microbiome composition of ankylosing spondylitis patients and the effect of traditional and herbal treatment.	0:179	Metagenome-wide association study of the alterations in the intestinal microbiome composition of ankylosing spondylitis patients and the effect of traditional and herbal treatment.
31778109	6	58	theme	nonsteroidal	932:943	arg1	celecoxib					975:983	e.g. celecoxib	970:983	e.g. celecoxib	970:983	Sixty-six faecal DNA samples, including 37 from healthy controls (HCs), 11 from patients with untreated AS (NM), 7 from patients treated with nonsteroidal anti-inflammatory drugs (e.g. celecoxib; WM) and 11 from patients treated with Chinese herbal medicine (CHM), such as the Bushen-Qiangdu-Zhilv decoction, were collected and used in the drug effect analysis.
31778109	6	58	theme	nonsteroidal	932:943	arg1	drugs					963:967	nonsteroidal anti-inflammatory drugs	932:967	nonsteroidal anti-inflammatory drugs (e.g. celecoxib; WM)	932:988	Sixty-six faecal DNA samples, including 37 from healthy controls (HCs), 11 from patients with untreated AS (NM), 7 from patients treated with nonsteroidal anti-inflammatory drugs (e.g. celecoxib; WM) and 11 from patients treated with Chinese herbal medicine (CHM), such as the Bushen-Qiangdu-Zhilv decoction, were collected and used in the drug effect analysis.
31778109	5	59	theme	AS	718:719	arg1	treatment					721:729	AS treatment	718:729	AS treatment	718:729	In parallel, the pathology of AS in each patient was analysed to better understand the link between AS treatment and the intestinal microbiota of the patients.Methodology.
31778109	10	60	theme	HC	1722:1723	arg1	groups					1732:1737	the HC and WM groups	1718:1737	groups	1732:1737	F. plautii was found to be significantly increased in the comparison between the HC and WM groups, along with four other species (Clostridium bolteae, Clostridiales bacterium 1_7_47FAA, C. asparagiforme and C. hathewayi).
31778109	11	61	theme	glycan	1961:1966	arg1	biosynthesis					1968:1979	glycan biosynthesis	1961:1979	glycan biosynthesis	1961:1979	The patients with AS harboured more bacterial species associated with carbohydrate metabolism and glycan biosynthesis in their faeces.
31778109	12	62	theme	bacterial	2012:2020	arg1	profiles					2022:2029	bacterial profiles	2012:2029	bacterial profiles less able to biodegrade xenobiotics or synthesize and transport vitamins.Conclusion	2012:2113	They also had bacterial profiles less able to biodegrade xenobiotics or synthesize and transport vitamins.Conclusion.
31778109	0	63	theme	herbal	163:168	arg1	treatment					170:178	traditional and herbal treatment	147:178	traditional and herbal treatment	147:178	Metagenome-wide association study of the alterations in the intestinal microbiome composition of ankylosing spondylitis patients and the effect of traditional and herbal treatment.
31778109	2	64	theme	systemic	228:235	arg1	disease					249:255	a systemic progressive disease	226:255	a systemic progressive disease with an unknown etiology that may be related to the gut microbiome	226:322	Ankylosing spondylitis (AS) is a systemic progressive disease with an unknown etiology that may be related to the gut microbiome.
31778109	2	64	theme	systemic	228:235	arg1	spondylitis					206:216	Ankylosing spondylitis	195:216	Ankylosing spondylitis (AS)	195:221	Ankylosing spondylitis (AS) is a systemic progressive disease with an unknown etiology that may be related to the gut microbiome.
31778109	8	65	dep	nordii	1393:1398	arg1	P<0.05					1412:1417	P<0.05	1412:1417	P<0.05	1412:1417	Four species were enriched in the patients with AS: Flavonifractor plautii, Oscillibacter, Parabacteroides distasonis and Bacteroides nordii (HC vs. NM, P<0.05); only F. plautii was found to be significantly changed in the NM-HC comparison.
31778109	8	65	dep	nordii	1393:1398	arg1	NM					1408:1409	HC vs. NM	1401:1409	NM	1408:1409	Four species were enriched in the patients with AS: Flavonifractor plautii, Oscillibacter, Parabacteroides distasonis and Bacteroides nordii (HC vs. NM, P<0.05); only F. plautii was found to be significantly changed in the NM-HC comparison.
31778109	8	65	dep	nordii	1393:1398	arg1	HC					1401:1402	HC vs. NM	1401:1409	HC	1401:1402	Four species were enriched in the patients with AS: Flavonifractor plautii, Oscillibacter, Parabacteroides distasonis and Bacteroides nordii (HC vs. NM, P<0.05); only F. plautii was found to be significantly changed in the NM-HC comparison.
31778109	0	66	theme	microbiome	71:80	arg1	composition					82:92	the intestinal microbiome composition	56:92	the intestinal microbiome composition of ankylosing spondylitis patients	56:127	Metagenome-wide association study of the alterations in the intestinal microbiome composition of ankylosing spondylitis patients and the effect of traditional and herbal treatment.
31778109	10	67	theme	WM	1729:1730	arg1	groups					1732:1737	the HC and WM groups	1718:1737	groups	1732:1737	F. plautii was found to be significantly increased in the comparison between the HC and WM groups, along with four other species (Clostridium bolteae, Clostridiales bacterium 1_7_47FAA, C. asparagiforme and C. hathewayi).
31778109	6	68	theme	effect	1135:1140	arg1	analysis					1142:1149	the drug effect analysis	1126:1149	the drug effect analysis	1126:1149	Sixty-six faecal DNA samples, including 37 from healthy controls (HCs), 11 from patients with untreated AS (NM), 7 from patients treated with nonsteroidal anti-inflammatory drugs (e.g. celecoxib; WM) and 11 from patients treated with Chinese herbal medicine (CHM), such as the Bushen-Qiangdu-Zhilv decoction, were collected and used in the drug effect analysis.
31778109	8	69	with	patients	1293:1300	arg1	AS					1307:1308	AS	1307:1308	AS	1307:1308	Four species were enriched in the patients with AS: Flavonifractor plautii, Oscillibacter, Parabacteroides distasonis and Bacteroides nordii (HC vs. NM, P<0.05); only F. plautii was found to be significantly changed in the NM-HC comparison.
31778109	5	70	theme	patients.Methodology	768:787	arg1	microbiota					750:759	the intestinal microbiota	735:759	the intestinal microbiota of the patients.Methodology	735:787	In parallel, the pathology of AS in each patient was analysed to better understand the link between AS treatment and the intestinal microbiota of the patients.Methodology.
31778109	5	70	theme	patients.Methodology	768:787	arg1	treatment					721:729	AS treatment	718:729	AS treatment	718:729	In parallel, the pathology of AS in each patient was analysed to better understand the link between AS treatment and the intestinal microbiota of the patients.Methodology.
31778109	2	71	with	disease	249:255	arg1	related					294:300	related	294:300	related	294:300	Ankylosing spondylitis (AS) is a systemic progressive disease with an unknown etiology that may be related to the gut microbiome.
31778109	2	71	with	disease	249:255	arg1	etiology					273:280	an unknown etiology	262:280	an unknown etiology that may be related to the gut microbiome	262:322	Ankylosing spondylitis (AS) is a systemic progressive disease with an unknown etiology that may be related to the gut microbiome.
31778109	0	72	theme	spondylitis	108:118	arg1	patients					120:127	ankylosing spondylitis patients	97:127	ankylosing spondylitis patients	97:127	Metagenome-wide association study of the alterations in the intestinal microbiome composition of ankylosing spondylitis patients and the effect of traditional and herbal treatment.
31778109	6	73	theme	e.g.	970:973	arg1	celecoxib					975:983	e.g. celecoxib	970:983	e.g. celecoxib	970:983	Sixty-six faecal DNA samples, including 37 from healthy controls (HCs), 11 from patients with untreated AS (NM), 7 from patients treated with nonsteroidal anti-inflammatory drugs (e.g. celecoxib; WM) and 11 from patients treated with Chinese herbal medicine (CHM), such as the Bushen-Qiangdu-Zhilv decoction, were collected and used in the drug effect analysis.
31778109	6	73	theme	e.g.	970:973	arg1	drugs					963:967	nonsteroidal anti-inflammatory drugs	932:967	nonsteroidal anti-inflammatory drugs (e.g. celecoxib; WM)	932:988	Sixty-six faecal DNA samples, including 37 from healthy controls (HCs), 11 from patients with untreated AS (NM), 7 from patients treated with nonsteroidal anti-inflammatory drugs (e.g. celecoxib; WM) and 11 from patients treated with Chinese herbal medicine (CHM), such as the Bushen-Qiangdu-Zhilv decoction, were collected and used in the drug effect analysis.
31778109	4	74	theme	microbial	485:493	arg1	composition					495:505	intestinal microbial composition	474:505	intestinal microbial composition	474:505	We aimed to determine the differences in intestinal microbial composition between healthy individuals and patients with AS who received and who did not receive treatment interventions.
31778109	5	75	theme	intestinal	739:748	arg1	microbiota					750:759	the intestinal microbiota	735:759	the intestinal microbiota of the patients.Methodology	735:787	In parallel, the pathology of AS in each patient was analysed to better understand the link between AS treatment and the intestinal microbiota of the patients.Methodology.
31778109	3	76	theme	thorough	343:350	arg1	necessary					389:397	necessary	389:397	necessary	389:397	Therefore, a more thorough understanding of its pathogenesis is necessary for directing future therapy.Aim.
31778109	3	76	theme	thorough	343:350	arg1	understanding					352:364	a more thorough understanding	336:364	a more thorough understanding of its pathogenesis	336:384	Therefore, a more thorough understanding of its pathogenesis is necessary for directing future therapy.Aim.
31778109	0	77	from	alterations	41:51	arg1	composition					82:92	the intestinal microbiome composition	56:92	the intestinal microbiome composition of ankylosing spondylitis patients	56:127	Metagenome-wide association study of the alterations in the intestinal microbiome composition of ankylosing spondylitis patients and the effect of traditional and herbal treatment.
31778109	10	78	dep	F.	1641:1642	arg1	plautii					1644:1650	F. plautii	1641:1650	F. plautii	1641:1650	F. plautii was found to be significantly increased in the comparison between the HC and WM groups, along with four other species (Clostridium bolteae, Clostridiales bacterium 1_7_47FAA, C. asparagiforme and C. hathewayi).
31778109	0	79	theme	association	16:26	arg1	study					28:32	Metagenome-wide association study	0:32	Metagenome-wide association study of the alterations in the intestinal microbiome composition of ankylosing spondylitis patients and the effect of traditional and herbal treatment.	0:179	Metagenome-wide association study of the alterations in the intestinal microbiome composition of ankylosing spondylitis patients and the effect of traditional and herbal treatment.
31778109	6	80	theme	herbal	1032:1037	arg1	CHM					1049:1051	CHM	1049:1051	CHM	1049:1051	Sixty-six faecal DNA samples, including 37 from healthy controls (HCs), 11 from patients with untreated AS (NM), 7 from patients treated with nonsteroidal anti-inflammatory drugs (e.g. celecoxib; WM) and 11 from patients treated with Chinese herbal medicine (CHM), such as the Bushen-Qiangdu-Zhilv decoction, were collected and used in the drug effect analysis.
31778109	6	80	theme	herbal	1032:1037	arg1	medicine					1039:1046	Chinese herbal medicine	1024:1046	Chinese herbal medicine (CHM)	1024:1052	Sixty-six faecal DNA samples, including 37 from healthy controls (HCs), 11 from patients with untreated AS (NM), 7 from patients treated with nonsteroidal anti-inflammatory drugs (e.g. celecoxib; WM) and 11 from patients treated with Chinese herbal medicine (CHM), such as the Bushen-Qiangdu-Zhilv decoction, were collected and used in the drug effect analysis.
31778109	9	81	located	found	1527:1531	arg2	species					1514:1520	No additional species	1500:1520	No additional species	1500:1520	No additional species were found in the HC vs. CHM analysis, which indicated a beneficial effect of CHM in removing the other three strains.
31778109	9	81	located	found	1527:1531	arg1	analysis					1551:1558	the HC vs. CHM analysis	1536:1558	the HC vs. CHM analysis	1536:1558	No additional species were found in the HC vs. CHM analysis, which indicated a beneficial effect of CHM in removing the other three strains.
31778109	8	82	theme	NM-HC	1482:1486	arg1	comparison					1488:1497	the NM-HC comparison	1478:1497	the NM-HC comparison	1478:1497	Four species were enriched in the patients with AS: Flavonifractor plautii, Oscillibacter, Parabacteroides distasonis and Bacteroides nordii (HC vs. NM, P<0.05); only F. plautii was found to be significantly changed in the NM-HC comparison.
31778109	3	83	theme	pathogenesis	373:384	arg1	necessary					389:397	necessary	389:397	necessary	389:397	Therefore, a more thorough understanding of its pathogenesis is necessary for directing future therapy.Aim.
31778109	3	83	theme	pathogenesis	373:384	arg1	understanding					352:364	a more thorough understanding	336:364	a more thorough understanding of its pathogenesis	336:384	Therefore, a more thorough understanding of its pathogenesis is necessary for directing future therapy.Aim.
31778109	10	84	theme	other	1756:1760	arg1	species					1762:1768	four other species	1751:1768	four other species (Clostridium bolteae, Clostridiales bacterium 1_7_47FAA, C. asparagiforme and C. hathewayi)	1751:1860	F. plautii was found to be significantly increased in the comparison between the HC and WM groups, along with four other species (Clostridium bolteae, Clostridiales bacterium 1_7_47FAA, C. asparagiforme and C. hathewayi).
31778109	0	85	theme	alterations	41:51	arg1	study					28:32	Metagenome-wide association study	0:32	Metagenome-wide association study of the alterations in the intestinal microbiome composition of ankylosing spondylitis patients and the effect of traditional and herbal treatment.	0:179	Metagenome-wide association study of the alterations in the intestinal microbiome composition of ankylosing spondylitis patients and the effect of traditional and herbal treatment.
31778109	8	86	dep	enriched	1277:1284	arg1	found					1441:1445	found	1441:1445	was found to be significantly changed in the NM-HC comparison	1437:1497	Four species were enriched in the patients with AS: Flavonifractor plautii, Oscillibacter, Parabacteroides distasonis and Bacteroides nordii (HC vs. NM, P<0.05); only F. plautii was found to be significantly changed in the NM-HC comparison.
31778109	5	87	from	patient	659:665	arg1	pathology					635:643	the pathology	631:643	the pathology of AS in each patient	631:665	In parallel, the pathology of AS in each patient was analysed to better understand the link between AS treatment and the intestinal microbiota of the patients.Methodology.
31778109	13	88	contain	had	2206:2208	arg1	treatment					2196:2204	the treatment	2192:2204	the treatment	2192:2204	The gut microbiota of the patients with AS varied from that of the HCs, and the treatment had an impact on this divergence.
31778109	13	88	contain	had	2206:2208	arg2	impact					2213:2218	an impact	2210:2218	an impact on this divergence	2210:2237	The gut microbiota of the patients with AS varied from that of the HCs, and the treatment had an impact on this divergence.
31778109	6	89	theme	healthy	838:844	arg1	HCs					856:858	HCs	856:858	HCs	856:858	Sixty-six faecal DNA samples, including 37 from healthy controls (HCs), 11 from patients with untreated AS (NM), 7 from patients treated with nonsteroidal anti-inflammatory drugs (e.g. celecoxib; WM) and 11 from patients treated with Chinese herbal medicine (CHM), such as the Bushen-Qiangdu-Zhilv decoction, were collected and used in the drug effect analysis.
31778109	6	89	theme	healthy	838:844	arg1	controls					846:853	healthy controls	838:853	healthy controls (HCs)	838:859	Sixty-six faecal DNA samples, including 37 from healthy controls (HCs), 11 from patients with untreated AS (NM), 7 from patients treated with nonsteroidal anti-inflammatory drugs (e.g. celecoxib; WM) and 11 from patients treated with Chinese herbal medicine (CHM), such as the Bushen-Qiangdu-Zhilv decoction, were collected and used in the drug effect analysis.
31778109	11	90	from	metabolism	1946:1955	arg1	faeces					1990:1995	their faeces	1984:1995	their faeces	1984:1995	The patients with AS harboured more bacterial species associated with carbohydrate metabolism and glycan biosynthesis in their faeces.
31778109	0	91	from	effect	137:142	arg1	composition					82:92	the intestinal microbiome composition	56:92	the intestinal microbiome composition of ankylosing spondylitis patients	56:127	Metagenome-wide association study of the alterations in the intestinal microbiome composition of ankylosing spondylitis patients and the effect of traditional and herbal treatment.
31778109	14	92	theme	AS	2298:2299	arg1	treatment					2301:2309	AS treatment	2298:2309	AS treatment	2298:2309	Our data provide insight that could guide improvements in AS treatment.
31012315	8	0	theme	phenol	1176:1181	arg1	oxidase					1183:1189	phenol oxidase	1176:1189	phenol oxidase	1176:1189	The activities of laccase, α-galactosidase, and phenol oxidase and the thermal stability of laccase were reduced after deglycosylation.
31012315	6	1	theme	pectin	1024:1029	arg1	lyase					1031:1035	pectin lyase	1024:1035	pectin lyase	1024:1035	N-Glycosylated peptides of laccase, GH92, exoglucanase, phenol oxidase, α-galactosidase, carboxylic ester hydrolase, and pectin lyase were identified.
31012315	8	2	theme	oxidase	1183:1189	arg1	stability					1207:1215	the thermal stability	1195:1215	the thermal stability of laccase	1195:1226	The activities of laccase, α-galactosidase, and phenol oxidase and the thermal stability of laccase were reduced after deglycosylation.
31012315	8	2	theme	oxidase	1183:1189	arg1	activities					1132:1141	The activities	1128:1141	The activities of laccase, α-galactosidase, and phenol oxidase	1128:1189	The activities of laccase, α-galactosidase, and phenol oxidase and the thermal stability of laccase were reduced after deglycosylation.
31012315	1	3	theme	stalks	253:258	arg1	pretreatment					221:232	pretreatment	221:232	pretreatment of industrial hemp stalks	221:258	Our previous research showed that Pleurotus eryngii and Pleurotus ostreatus were effective fungi for pretreatment of industrial hemp stalks to improve enzymatic saccharification.
31012315	6	4	theme	laccase	930:936	arg1	peptides					918:925	N-Glycosylated peptides	903:925	N-Glycosylated peptides of laccase, GH92, exoglucanase, phenol oxidase, α-galactosidase, carboxylic ester hydrolase, and pectin lyase	903:1035	N-Glycosylated peptides of laccase, GH92, exoglucanase, phenol oxidase, α-galactosidase, carboxylic ester hydrolase, and pectin lyase were identified.
31012315	7	5	theme	stalks	1120:1125	arg1	saccharification					1095:1110	enzymatic saccharification	1085:1110	enzymatic saccharification of hemp stalks	1085:1125	Deglycosylation could decrease enzymatic saccharification of hemp stalks.
31012315	4	6	theme	glycosylated	696:707	arg1	proteins					665:672	the extracellular proteins	647:672	the extracellular proteins secreted by fungi	647:690	Because most of the extracellular proteins secreted by fungi are glycosylated proteins, the N-linked glycosylation of enzymes could be mapped.
31012315	4	6	theme	glycosylated	696:707	arg1	proteins					709:716	glycosylated proteins	696:716	glycosylated proteins	696:716	Because most of the extracellular proteins secreted by fungi are glycosylated proteins, the N-linked glycosylation of enzymes could be mapped.
31012315	4	6	theme	glycosylated	696:707	arg1	most					639:642	most	639:642	most	639:642	Because most of the extracellular proteins secreted by fungi are glycosylated proteins, the N-linked glycosylation of enzymes could be mapped.
31012315	6	7	theme	hydrolase	1009:1017	arg1	peptides					918:925	N-Glycosylated peptides	903:925	N-Glycosylated peptides of laccase, GH92, exoglucanase, phenol oxidase, α-galactosidase, carboxylic ester hydrolase, and pectin lyase	903:1035	N-Glycosylated peptides of laccase, GH92, exoglucanase, phenol oxidase, α-galactosidase, carboxylic ester hydrolase, and pectin lyase were identified.
31012315	5	8	dep	Pleurotus	857:865	arg1	ostreatus					867:875	ostreatus	867:875	ostreatus	867:875	In total, 27 and 24 N-glycosylated peptides were detected in Pleurotus eryngii and Pleurotus ostreatus secretomes, respectively.
31012315	6	9	theme	ester	1003:1007	arg1	hydrolase					1009:1017	carboxylic ester hydrolase	992:1017	carboxylic ester hydrolase	992:1017	N-Glycosylated peptides of laccase, GH92, exoglucanase, phenol oxidase, α-galactosidase, carboxylic ester hydrolase, and pectin lyase were identified.
31012315	4	10	theme	enzymes	749:755	arg1	glycosylation					732:744	the N-linked glycosylation	719:744	the N-linked glycosylation of enzymes	719:755	Because most of the extracellular proteins secreted by fungi are glycosylated proteins, the N-linked glycosylation of enzymes could be mapped.
31012315	5	11	located	detected	823:830	arg2	peptides					809:816	27 and 24 N-glycosylated peptides	784:816	27 and 24 N-glycosylated peptides	784:816	In total, 27 and 24 N-glycosylated peptides were detected in Pleurotus eryngii and Pleurotus ostreatus secretomes, respectively.
31012315	5	11	located	detected	823:830	arg1	secretomes					877:886	Pleurotus eryngii and Pleurotus ostreatus secretomes	835:886	Pleurotus eryngii and Pleurotus ostreatus secretomes	835:886	In total, 27 and 24 N-glycosylated peptides were detected in Pleurotus eryngii and Pleurotus ostreatus secretomes, respectively.
31012315	5	11	located	detected	823:830	arg1	total					777:781	total	777:781	total	777:781	In total, 27 and 24 N-glycosylated peptides were detected in Pleurotus eryngii and Pleurotus ostreatus secretomes, respectively.
31012315	4	12	link	N-linked	723:730	arg1	glycosylation					732:744	the N-linked glycosylation	719:744	the N-linked glycosylation of enzymes	719:755	Because most of the extracellular proteins secreted by fungi are glycosylated proteins, the N-linked glycosylation of enzymes could be mapped.
31012315	1	13	theme	enzymatic	271:279	arg1	saccharification					281:296	enzymatic saccharification	271:296	enzymatic saccharification	271:296	Our previous research showed that Pleurotus eryngii and Pleurotus ostreatus were effective fungi for pretreatment of industrial hemp stalks to improve enzymatic saccharification.
31012315	0	14	theme	Hemp	107:110	arg1	Stalks					112:117	Hemp Stalks	107:117	Hemp Stalks	107:117	Mapping the Secretome and Its N-Linked Glycosylation of Pleurotus eryngii and Pleurotus ostreatus Grown on Hemp Stalks.
31012315	6	15	theme	N-Glycosylated	903:916	arg1	peptides					918:925	N-Glycosylated peptides	903:925	N-Glycosylated peptides of laccase, GH92, exoglucanase, phenol oxidase, α-galactosidase, carboxylic ester hydrolase, and pectin lyase	903:1035	N-Glycosylated peptides of laccase, GH92, exoglucanase, phenol oxidase, α-galactosidase, carboxylic ester hydrolase, and pectin lyase were identified.
31012315	5	16	theme	Pleurotus	835:843	arg1	secretomes					877:886	Pleurotus eryngii and Pleurotus ostreatus secretomes	835:886	Pleurotus eryngii and Pleurotus ostreatus secretomes	835:886	In total, 27 and 24 N-glycosylated peptides were detected in Pleurotus eryngii and Pleurotus ostreatus secretomes, respectively.
31012315	1	17	theme	effective	201:209	arg1	fungi					211:215	effective fungi	201:215	effective fungi	201:215	Our previous research showed that Pleurotus eryngii and Pleurotus ostreatus were effective fungi for pretreatment of industrial hemp stalks to improve enzymatic saccharification.
31012315	5	18	theme	Pleurotus	857:865	arg1	secretomes					877:886	Pleurotus eryngii and Pleurotus ostreatus secretomes	835:886	Pleurotus eryngii and Pleurotus ostreatus secretomes	835:886	In total, 27 and 24 N-glycosylated peptides were detected in Pleurotus eryngii and Pleurotus ostreatus secretomes, respectively.
31012315	6	19	theme	carboxylic	992:1001	arg1	hydrolase					1009:1017	carboxylic ester hydrolase	992:1017	carboxylic ester hydrolase	992:1017	N-Glycosylated peptides of laccase, GH92, exoglucanase, phenol oxidase, α-galactosidase, carboxylic ester hydrolase, and pectin lyase were identified.
31012315	3	20	dep	Pleurotus	497:505	arg1	eryngii					507:513	eryngii	507:513	eryngii	507:513	In total, 169 and 155 proteins were identified in Pleurotus eryngii and Pleurotus ostreatus, respectively, and 50% of the proteins involved in lignocellulose degradation were CAZymes.
31012315	2	21	theme	hemp	402:405	arg1	lignin					407:412	hemp lignin	402:412	hemp lignin	402:412	The secretomes of these two fungi were analyzed to search for the effective enzyme cocktails degrading hemp lignin during the pretreatment process.
31012315	5	22	theme	N-glycosylated	794:807	arg1	peptides					809:816	27 and 24 N-glycosylated peptides	784:816	27 and 24 N-glycosylated peptides	784:816	In total, 27 and 24 N-glycosylated peptides were detected in Pleurotus eryngii and Pleurotus ostreatus secretomes, respectively.
31012315	7	23	theme	hemp	1115:1118	arg1	stalks					1120:1125	hemp stalks	1115:1125	hemp stalks	1115:1125	Deglycosylation could decrease enzymatic saccharification of hemp stalks.
31012315	2	24	theme	fungi	327:331	arg1	secretomes					303:312	The secretomes	299:312	The secretomes of these two fungi	299:331	The secretomes of these two fungi were analyzed to search for the effective enzyme cocktails degrading hemp lignin during the pretreatment process.
31012315	4	25	theme	N-linked	723:730	arg1	glycosylation					732:744	the N-linked glycosylation	719:744	the N-linked glycosylation of enzymes	719:755	Because most of the extracellular proteins secreted by fungi are glycosylated proteins, the N-linked glycosylation of enzymes could be mapped.
31012315	6	26	theme	α-galactosidase	975:989	arg1	peptides					918:925	N-Glycosylated peptides	903:925	N-Glycosylated peptides of laccase, GH92, exoglucanase, phenol oxidase, α-galactosidase, carboxylic ester hydrolase, and pectin lyase	903:1035	N-Glycosylated peptides of laccase, GH92, exoglucanase, phenol oxidase, α-galactosidase, carboxylic ester hydrolase, and pectin lyase were identified.
31012315	3	27	dep	Pleurotus	519:527	arg1	ostreatus					529:537	ostreatus	529:537	ostreatus	529:537	In total, 169 and 155 proteins were identified in Pleurotus eryngii and Pleurotus ostreatus, respectively, and 50% of the proteins involved in lignocellulose degradation were CAZymes.
31012315	8	28	theme	laccase	1220:1226	arg1	stability					1207:1215	the thermal stability	1195:1215	the thermal stability of laccase	1195:1226	The activities of laccase, α-galactosidase, and phenol oxidase and the thermal stability of laccase were reduced after deglycosylation.
31012315	8	28	theme	laccase	1220:1226	arg1	activities					1132:1141	The activities	1128:1141	The activities of laccase, α-galactosidase, and phenol oxidase	1128:1189	The activities of laccase, α-galactosidase, and phenol oxidase and the thermal stability of laccase were reduced after deglycosylation.
31012315	2	29	theme	pretreatment	425:436	arg1	process					438:444	the pretreatment process	421:444	the pretreatment process	421:444	The secretomes of these two fungi were analyzed to search for the effective enzyme cocktails degrading hemp lignin during the pretreatment process.
31012315	0	30	theme	N-Linked	30:37	arg1	Glycosylation					39:51	Its N-Linked Glycosylation	26:51	Its N-Linked Glycosylation	26:51	Mapping the Secretome and Its N-Linked Glycosylation of Pleurotus eryngii and Pleurotus ostreatus Grown on Hemp Stalks.
31012315	6	31	theme	oxidase	966:972	arg1	peptides					918:925	N-Glycosylated peptides	903:925	N-Glycosylated peptides of laccase, GH92, exoglucanase, phenol oxidase, α-galactosidase, carboxylic ester hydrolase, and pectin lyase	903:1035	N-Glycosylated peptides of laccase, GH92, exoglucanase, phenol oxidase, α-galactosidase, carboxylic ester hydrolase, and pectin lyase were identified.
31012315	6	32	theme	phenol	959:964	arg1	oxidase					966:972	phenol oxidase	959:972	phenol oxidase	959:972	N-Glycosylated peptides of laccase, GH92, exoglucanase, phenol oxidase, α-galactosidase, carboxylic ester hydrolase, and pectin lyase were identified.
31012315	4	33	gly	glycosylation	732:744	arg1	enzymes					749:755	enzymes	749:755	enzymes	749:755	Because most of the extracellular proteins secreted by fungi are glycosylated proteins, the N-linked glycosylation of enzymes could be mapped.
31012315	3	34	theme	lignocellulose	590:603	arg1	degradation					605:615	lignocellulose degradation	590:615	lignocellulose degradation	590:615	In total, 169 and 155 proteins were identified in Pleurotus eryngii and Pleurotus ostreatus, respectively, and 50% of the proteins involved in lignocellulose degradation were CAZymes.
31012315	3	35	theme	proteins	569:576	arg1	%					560:560	50%	558:560	50% of the proteins involved in lignocellulose degradation	558:615	In total, 169 and 155 proteins were identified in Pleurotus eryngii and Pleurotus ostreatus, respectively, and 50% of the proteins involved in lignocellulose degradation were CAZymes.
31012315	3	35	theme	proteins	569:576	arg1	proteins					569:576	the proteins	565:576	the proteins involved in lignocellulose degradation	565:615	In total, 169 and 155 proteins were identified in Pleurotus eryngii and Pleurotus ostreatus, respectively, and 50% of the proteins involved in lignocellulose degradation were CAZymes.
31012315	7	36	theme	enzymatic	1085:1093	arg1	saccharification					1095:1110	enzymatic saccharification	1085:1110	enzymatic saccharification of hemp stalks	1085:1125	Deglycosylation could decrease enzymatic saccharification of hemp stalks.
31012315	6	37	theme	exoglucanase	945:956	arg1	peptides					918:925	N-Glycosylated peptides	903:925	N-Glycosylated peptides of laccase, GH92, exoglucanase, phenol oxidase, α-galactosidase, carboxylic ester hydrolase, and pectin lyase	903:1035	N-Glycosylated peptides of laccase, GH92, exoglucanase, phenol oxidase, α-galactosidase, carboxylic ester hydrolase, and pectin lyase were identified.
31012315	4	38	gly	glycosylated	696:707	arg1	proteins					665:672	the extracellular proteins	647:672	the extracellular proteins secreted by fungi	647:690	Because most of the extracellular proteins secreted by fungi are glycosylated proteins, the N-linked glycosylation of enzymes could be mapped.
31012315	4	38	gly	glycosylated	696:707	arg1	proteins					709:716	glycosylated proteins	696:716	glycosylated proteins	696:716	Because most of the extracellular proteins secreted by fungi are glycosylated proteins, the N-linked glycosylation of enzymes could be mapped.
31012315	4	38	gly	glycosylated	696:707	arg1	most					639:642	most	639:642	most	639:642	Because most of the extracellular proteins secreted by fungi are glycosylated proteins, the N-linked glycosylation of enzymes could be mapped.
31012315	5	39	gly	N-glycosylated	794:807	arg1	peptides					809:816	27 and 24 N-glycosylated peptides	784:816	27 and 24 N-glycosylated peptides	784:816	In total, 27 and 24 N-glycosylated peptides were detected in Pleurotus eryngii and Pleurotus ostreatus secretomes, respectively.
31012315	8	40	theme	laccase	1146:1152	arg1	stability					1207:1215	the thermal stability	1195:1215	the thermal stability of laccase	1195:1226	The activities of laccase, α-galactosidase, and phenol oxidase and the thermal stability of laccase were reduced after deglycosylation.
31012315	8	40	theme	laccase	1146:1152	arg1	activities					1132:1141	The activities	1128:1141	The activities of laccase, α-galactosidase, and phenol oxidase	1128:1189	The activities of laccase, α-galactosidase, and phenol oxidase and the thermal stability of laccase were reduced after deglycosylation.
31012315	0	41	dep	Secretome	12:20	arg1	the					8:10	the	8:10	the	8:10	Mapping the Secretome and Its N-Linked Glycosylation of Pleurotus eryngii and Pleurotus ostreatus Grown on Hemp Stalks.
31012315	1	42	theme	previous	124:131	arg1	research					133:140	Our previous research	120:140	Our previous research	120:140	Our previous research showed that Pleurotus eryngii and Pleurotus ostreatus were effective fungi for pretreatment of industrial hemp stalks to improve enzymatic saccharification.
31012315	6	43	theme	lyase	1031:1035	arg1	peptides					918:925	N-Glycosylated peptides	903:925	N-Glycosylated peptides of laccase, GH92, exoglucanase, phenol oxidase, α-galactosidase, carboxylic ester hydrolase, and pectin lyase	903:1035	N-Glycosylated peptides of laccase, GH92, exoglucanase, phenol oxidase, α-galactosidase, carboxylic ester hydrolase, and pectin lyase were identified.
31012315	0	44	theme	eryngii	66:72	arg1	Secretome					12:20	Secretome	12:20	Secretome	12:20	Mapping the Secretome and Its N-Linked Glycosylation of Pleurotus eryngii and Pleurotus ostreatus Grown on Hemp Stalks.
31012315	0	44	theme	eryngii	66:72	arg1	Glycosylation					39:51	Its N-Linked Glycosylation	26:51	Its N-Linked Glycosylation	26:51	Mapping the Secretome and Its N-Linked Glycosylation of Pleurotus eryngii and Pleurotus ostreatus Grown on Hemp Stalks.
31012315	0	45	gly	Glycosylation	39:51	arg1	eryngii					66:72	Pleurotus eryngii	56:72	Pleurotus eryngii	56:72	Mapping the Secretome and Its N-Linked Glycosylation of Pleurotus eryngii and Pleurotus ostreatus Grown on Hemp Stalks.
31012315	8	46	theme	thermal	1199:1205	arg1	stability					1207:1215	the thermal stability	1195:1215	the thermal stability of laccase	1195:1226	The activities of laccase, α-galactosidase, and phenol oxidase and the thermal stability of laccase were reduced after deglycosylation.
31012315	2	47	theme	enzyme	375:380	arg1	cocktails					382:390	the effective enzyme cocktails	361:390	the effective enzyme cocktails degrading hemp lignin during the pretreatment process	361:444	The secretomes of these two fungi were analyzed to search for the effective enzyme cocktails degrading hemp lignin during the pretreatment process.
31012315	4	48	theme	extracellular	651:663	arg1	proteins					665:672	the extracellular proteins	647:672	the extracellular proteins secreted by fungi	647:690	Because most of the extracellular proteins secreted by fungi are glycosylated proteins, the N-linked glycosylation of enzymes could be mapped.
31012315	1	49	theme	industrial	237:246	arg1	stalks					253:258	industrial hemp stalks	237:258	industrial hemp stalks	237:258	Our previous research showed that Pleurotus eryngii and Pleurotus ostreatus were effective fungi for pretreatment of industrial hemp stalks to improve enzymatic saccharification.
31012315	8	50	theme	α-galactosidase	1155:1169	arg1	stability					1207:1215	the thermal stability	1195:1215	the thermal stability of laccase	1195:1226	The activities of laccase, α-galactosidase, and phenol oxidase and the thermal stability of laccase were reduced after deglycosylation.
31012315	8	50	theme	α-galactosidase	1155:1169	arg1	activities					1132:1141	The activities	1128:1141	The activities of laccase, α-galactosidase, and phenol oxidase	1128:1189	The activities of laccase, α-galactosidase, and phenol oxidase and the thermal stability of laccase were reduced after deglycosylation.
31012315	6	51	theme	GH92	939:942	arg1	peptides					918:925	N-Glycosylated peptides	903:925	N-Glycosylated peptides of laccase, GH92, exoglucanase, phenol oxidase, α-galactosidase, carboxylic ester hydrolase, and pectin lyase	903:1035	N-Glycosylated peptides of laccase, GH92, exoglucanase, phenol oxidase, α-galactosidase, carboxylic ester hydrolase, and pectin lyase were identified.
31012315	5	52	dep	Pleurotus	835:843	arg1	eryngii					845:851	eryngii	845:851	eryngii	845:851	In total, 27 and 24 N-glycosylated peptides were detected in Pleurotus eryngii and Pleurotus ostreatus secretomes, respectively.
31012315	2	53	theme	effective	365:373	arg1	cocktails					382:390	the effective enzyme cocktails	361:390	the effective enzyme cocktails degrading hemp lignin during the pretreatment process	361:444	The secretomes of these two fungi were analyzed to search for the effective enzyme cocktails degrading hemp lignin during the pretreatment process.
31012315	1	54	theme	hemp	248:251	arg1	stalks					253:258	industrial hemp stalks	237:258	industrial hemp stalks	237:258	Our previous research showed that Pleurotus eryngii and Pleurotus ostreatus were effective fungi for pretreatment of industrial hemp stalks to improve enzymatic saccharification.
31826397	3	0	from	analyses	753:760	arg1	characterizations					799:815	macromolecular and conformational characterizations	765:815	macromolecular and conformational characterizations	765:815	Furthermore, based on macromolecular models, comprehensive analyses on macromolecular and conformational characterizations of PFPP fractions were conducted quantitatively through, e.g., shape factor (1.42-1.79), Mark-Houwink-Sakurada exponent (0.55-0.74), conformational power-law exponent (0.52-0.58), fractal dimension (1.72-1.94) and persistence length (6.73-13.47 nm).
31826397	5	1	theme	molecular	1235:1243	arg1	basis					1245:1249	a molecular basis	1233:1249	a molecular basis for precise re-utilizations of PFPP in food and pharmaceutical industries	1233:1323	This could provide a molecular basis for precise re-utilizations of PFPP in food and pharmaceutical industries.
31826397	2	2	theme	composition	429:439	arg1	analyses					441:448	composition analyses	429:448	composition analyses	429:448	According to composition analyses, pectic PFPP and its fractions (PFPP-10, -15 and -20) were revealed as linear homogalacturonans interrupted by rhamnogalacturonan I in different lengths and extensities, where low esterification degrees (35.35-39.66%) were indicated via FT-IR.
31826397	2	3	theme	linear	521:526	arg1	homogalacturonans					528:544	linear homogalacturonans	521:544	linear homogalacturonans interrupted by rhamnogalacturonan I in different lengths and extensities	521:617	According to composition analyses, pectic PFPP and its fractions (PFPP-10, -15 and -20) were revealed as linear homogalacturonans interrupted by rhamnogalacturonan I in different lengths and extensities, where low esterification degrees (35.35-39.66%) were indicated via FT-IR.
31826397	2	3	theme	linear	521:526	arg1	PFPP					458:461	pectic PFPP	451:461	pectic PFPP	451:461	According to composition analyses, pectic PFPP and its fractions (PFPP-10, -15 and -20) were revealed as linear homogalacturonans interrupted by rhamnogalacturonan I in different lengths and extensities, where low esterification degrees (35.35-39.66%) were indicated via FT-IR.
31826397	2	3	theme	linear	521:526	arg1	fractions					471:479	its fractions	467:479	its fractions (PFPP-10, -15 and -20)	467:502	According to composition analyses, pectic PFPP and its fractions (PFPP-10, -15 and -20) were revealed as linear homogalacturonans interrupted by rhamnogalacturonan I in different lengths and extensities, where low esterification degrees (35.35-39.66%) were indicated via FT-IR.
31826397	1	4	theme	extraction	388:397	arg1	time					399:402	30 min extraction time	381:402	30 min extraction time	381:402	A polysaccharide (PFPP) from purple passion fruit peel was optimally extracted, with the highest yield (10.05%, w/w) obtained under 35 °C extraction temperature, 240 W ultrasonic power, 65:1 mL/g liquid-to-solid ratio, 0.6% (w/v) ammonium oxalate, 30 min extraction time and pH 2.0.
31826397	1	5	theme	purple	162:167	arg1	peel					183:186	purple passion fruit peel	162:186	purple passion fruit peel	162:186	A polysaccharide (PFPP) from purple passion fruit peel was optimally extracted, with the highest yield (10.05%, w/w) obtained under 35 °C extraction temperature, 240 W ultrasonic power, 65:1 mL/g liquid-to-solid ratio, 0.6% (w/v) ammonium oxalate, 30 min extraction time and pH 2.0.
31826397	3	6	theme	conformational	784:797	arg1	characterizations					799:815	macromolecular and conformational characterizations	765:815	macromolecular and conformational characterizations	765:815	Furthermore, based on macromolecular models, comprehensive analyses on macromolecular and conformational characterizations of PFPP fractions were conducted quantitatively through, e.g., shape factor (1.42-1.79), Mark-Houwink-Sakurada exponent (0.55-0.74), conformational power-law exponent (0.52-0.58), fractal dimension (1.72-1.94) and persistence length (6.73-13.47 nm).
31826397	1	7	theme	65:1 mL/g	319:327	arg1	ratio					345:349	65:1 mL/g liquid-to-solid ratio	319:349	65:1 mL/g liquid-to-solid ratio	319:349	A polysaccharide (PFPP) from purple passion fruit peel was optimally extracted, with the highest yield (10.05%, w/w) obtained under 35 °C extraction temperature, 240 W ultrasonic power, 65:1 mL/g liquid-to-solid ratio, 0.6% (w/v) ammonium oxalate, 30 min extraction time and pH 2.0.
31826397	3	8	theme	power-law	965:973	arg1	0.52-0.58					985:993	0.52-0.58	985:993	0.52-0.58	985:993	Furthermore, based on macromolecular models, comprehensive analyses on macromolecular and conformational characterizations of PFPP fractions were conducted quantitatively through, e.g., shape factor (1.42-1.79), Mark-Houwink-Sakurada exponent (0.55-0.74), conformational power-law exponent (0.52-0.58), fractal dimension (1.72-1.94) and persistence length (6.73-13.47 nm).
31826397	3	8	theme	power-law	965:973	arg1	exponent					975:982	conformational power-law exponent	950:982	conformational power-law exponent (0.52-0.58)	950:994	Furthermore, based on macromolecular models, comprehensive analyses on macromolecular and conformational characterizations of PFPP fractions were conducted quantitatively through, e.g., shape factor (1.42-1.79), Mark-Houwink-Sakurada exponent (0.55-0.74), conformational power-law exponent (0.52-0.58), fractal dimension (1.72-1.94) and persistence length (6.73-13.47 nm).
31826397	3	8	theme	power-law	965:973	arg1	factor					886:891	shape factor	880:891	shape factor (1.42-1.79)	880:903	Furthermore, based on macromolecular models, comprehensive analyses on macromolecular and conformational characterizations of PFPP fractions were conducted quantitatively through, e.g., shape factor (1.42-1.79), Mark-Houwink-Sakurada exponent (0.55-0.74), conformational power-law exponent (0.52-0.58), fractal dimension (1.72-1.94) and persistence length (6.73-13.47 nm).
31826397	0	9	theme	macromolecular	80:93	arg1	characterizations					114:130	macromolecular and conformational characterizations	80:130	macromolecular and conformational characterizations	80:130	Pectic polysaccharides from purple passion fruit peel: A comprehensive study in macromolecular and conformational characterizations.
31826397	1	10	theme	liquid-to-solid	329:343	arg1	ratio					345:349	65:1 mL/g liquid-to-solid ratio	319:349	65:1 mL/g liquid-to-solid ratio	319:349	A polysaccharide (PFPP) from purple passion fruit peel was optimally extracted, with the highest yield (10.05%, w/w) obtained under 35 °C extraction temperature, 240 W ultrasonic power, 65:1 mL/g liquid-to-solid ratio, 0.6% (w/v) ammonium oxalate, 30 min extraction time and pH 2.0.
31826397	2	11	theme	different	585:593	arg1	lengths					595:601	different lengths	585:601	different lengths	585:601	According to composition analyses, pectic PFPP and its fractions (PFPP-10, -15 and -20) were revealed as linear homogalacturonans interrupted by rhamnogalacturonan I in different lengths and extensities, where low esterification degrees (35.35-39.66%) were indicated via FT-IR.
31826397	4	12	theme	semi-flexible	1088:1100	arg1	conformations					1107:1119	different semi-flexible coil conformations	1078:1119	different semi-flexible coil conformations	1078:1119	Therefore, different semi-flexible coil conformations were proposed schematically, where lower molecular-weight PFPP fractions were less flexible.
31826397	3	13	theme	macromolecular	765:778	arg1	characterizations					799:815	macromolecular and conformational characterizations	765:815	macromolecular and conformational characterizations	765:815	Furthermore, based on macromolecular models, comprehensive analyses on macromolecular and conformational characterizations of PFPP fractions were conducted quantitatively through, e.g., shape factor (1.42-1.79), Mark-Houwink-Sakurada exponent (0.55-0.74), conformational power-law exponent (0.52-0.58), fractal dimension (1.72-1.94) and persistence length (6.73-13.47 nm).
31826397	0	14	from	peel	49:52	arg1	polysaccharides					7:21	Pectic polysaccharides	0:21	Pectic polysaccharides from purple passion fruit peel: A comprehensive study in macromolecular and conformational characterizations.	0:131	Pectic polysaccharides from purple passion fruit peel: A comprehensive study in macromolecular and conformational characterizations.
31826397	3	15	theme	shape	880:884	arg1	1.42-1.79					894:902	1.42-1.79	894:902	1.42-1.79	894:902	Furthermore, based on macromolecular models, comprehensive analyses on macromolecular and conformational characterizations of PFPP fractions were conducted quantitatively through, e.g., shape factor (1.42-1.79), Mark-Houwink-Sakurada exponent (0.55-0.74), conformational power-law exponent (0.52-0.58), fractal dimension (1.72-1.94) and persistence length (6.73-13.47 nm).
31826397	3	15	theme	shape	880:884	arg1	dimension					1005:1013	fractal dimension	997:1013	fractal dimension (1.72-1.94)	997:1025	Furthermore, based on macromolecular models, comprehensive analyses on macromolecular and conformational characterizations of PFPP fractions were conducted quantitatively through, e.g., shape factor (1.42-1.79), Mark-Houwink-Sakurada exponent (0.55-0.74), conformational power-law exponent (0.52-0.58), fractal dimension (1.72-1.94) and persistence length (6.73-13.47 nm).
31826397	3	15	theme	shape	880:884	arg1	exponent					928:935	Mark-Houwink-Sakurada exponent	906:935	Mark-Houwink-Sakurada exponent (0.55-0.74)	906:947	Furthermore, based on macromolecular models, comprehensive analyses on macromolecular and conformational characterizations of PFPP fractions were conducted quantitatively through, e.g., shape factor (1.42-1.79), Mark-Houwink-Sakurada exponent (0.55-0.74), conformational power-law exponent (0.52-0.58), fractal dimension (1.72-1.94) and persistence length (6.73-13.47 nm).
31826397	3	15	theme	shape	880:884	arg1	length					1043:1048	persistence length	1031:1048	persistence length	1031:1048	Furthermore, based on macromolecular models, comprehensive analyses on macromolecular and conformational characterizations of PFPP fractions were conducted quantitatively through, e.g., shape factor (1.42-1.79), Mark-Houwink-Sakurada exponent (0.55-0.74), conformational power-law exponent (0.52-0.58), fractal dimension (1.72-1.94) and persistence length (6.73-13.47 nm).
31826397	3	15	theme	shape	880:884	arg1	exponent					975:982	conformational power-law exponent	950:982	conformational power-law exponent (0.52-0.58)	950:994	Furthermore, based on macromolecular models, comprehensive analyses on macromolecular and conformational characterizations of PFPP fractions were conducted quantitatively through, e.g., shape factor (1.42-1.79), Mark-Houwink-Sakurada exponent (0.55-0.74), conformational power-law exponent (0.52-0.58), fractal dimension (1.72-1.94) and persistence length (6.73-13.47 nm).
31826397	3	15	theme	shape	880:884	arg1	factor					886:891	shape factor	880:891	shape factor (1.42-1.79)	880:903	Furthermore, based on macromolecular models, comprehensive analyses on macromolecular and conformational characterizations of PFPP fractions were conducted quantitatively through, e.g., shape factor (1.42-1.79), Mark-Houwink-Sakurada exponent (0.55-0.74), conformational power-law exponent (0.52-0.58), fractal dimension (1.72-1.94) and persistence length (6.73-13.47 nm).
31826397	1	16	theme	passion	169:175	arg1	peel					183:186	purple passion fruit peel	162:186	purple passion fruit peel	162:186	A polysaccharide (PFPP) from purple passion fruit peel was optimally extracted, with the highest yield (10.05%, w/w) obtained under 35 °C extraction temperature, 240 W ultrasonic power, 65:1 mL/g liquid-to-solid ratio, 0.6% (w/v) ammonium oxalate, 30 min extraction time and pH 2.0.
31826397	0	17	theme	conformational	99:112	arg1	characterizations					114:130	macromolecular and conformational characterizations	80:130	macromolecular and conformational characterizations	80:130	Pectic polysaccharides from purple passion fruit peel: A comprehensive study in macromolecular and conformational characterizations.
31826397	2	18	theme	low	626:628	arg1	degrees					645:651	low esterification degrees	626:651	low esterification degrees (35.35-39.66%)	626:666	According to composition analyses, pectic PFPP and its fractions (PFPP-10, -15 and -20) were revealed as linear homogalacturonans interrupted by rhamnogalacturonan I in different lengths and extensities, where low esterification degrees (35.35-39.66%) were indicated via FT-IR.
31826397	2	18	theme	low	626:628	arg1	%					665:665	35.35-39.66%	654:665	35.35-39.66%	654:665	According to composition analyses, pectic PFPP and its fractions (PFPP-10, -15 and -20) were revealed as linear homogalacturonans interrupted by rhamnogalacturonan I in different lengths and extensities, where low esterification degrees (35.35-39.66%) were indicated via FT-IR.
31826397	1	19	theme	fruit	177:181	arg1	peel					183:186	purple passion fruit peel	162:186	purple passion fruit peel	162:186	A polysaccharide (PFPP) from purple passion fruit peel was optimally extracted, with the highest yield (10.05%, w/w) obtained under 35 °C extraction temperature, 240 W ultrasonic power, 65:1 mL/g liquid-to-solid ratio, 0.6% (w/v) ammonium oxalate, 30 min extraction time and pH 2.0.
31826397	5	20	theme	precise	1255:1261	arg1	re-utilizations					1263:1277	precise re-utilizations	1255:1277	precise re-utilizations of PFPP in food and pharmaceutical industries	1255:1323	This could provide a molecular basis for precise re-utilizations of PFPP in food and pharmaceutical industries.
31826397	1	21	theme	0.6	352:354	arg1	%					355:355	%	355:355	%	355:355	A polysaccharide (PFPP) from purple passion fruit peel was optimally extracted, with the highest yield (10.05%, w/w) obtained under 35 °C extraction temperature, 240 W ultrasonic power, 65:1 mL/g liquid-to-solid ratio, 0.6% (w/v) ammonium oxalate, 30 min extraction time and pH 2.0.
31826397	3	22	theme	persistence	1031:1041	arg1	length					1043:1048	persistence length	1031:1048	persistence length	1031:1048	Furthermore, based on macromolecular models, comprehensive analyses on macromolecular and conformational characterizations of PFPP fractions were conducted quantitatively through, e.g., shape factor (1.42-1.79), Mark-Houwink-Sakurada exponent (0.55-0.74), conformational power-law exponent (0.52-0.58), fractal dimension (1.72-1.94) and persistence length (6.73-13.47 nm).
31826397	3	22	theme	persistence	1031:1041	arg1	factor					886:891	shape factor	880:891	shape factor (1.42-1.79)	880:903	Furthermore, based on macromolecular models, comprehensive analyses on macromolecular and conformational characterizations of PFPP fractions were conducted quantitatively through, e.g., shape factor (1.42-1.79), Mark-Houwink-Sakurada exponent (0.55-0.74), conformational power-law exponent (0.52-0.58), fractal dimension (1.72-1.94) and persistence length (6.73-13.47 nm).
31826397	2	23	theme	pectic	451:456	arg1	homogalacturonans					528:544	linear homogalacturonans	521:544	linear homogalacturonans interrupted by rhamnogalacturonan I in different lengths and extensities	521:617	According to composition analyses, pectic PFPP and its fractions (PFPP-10, -15 and -20) were revealed as linear homogalacturonans interrupted by rhamnogalacturonan I in different lengths and extensities, where low esterification degrees (35.35-39.66%) were indicated via FT-IR.
31826397	2	23	theme	pectic	451:456	arg1	PFPP					458:461	pectic PFPP	451:461	pectic PFPP	451:461	According to composition analyses, pectic PFPP and its fractions (PFPP-10, -15 and -20) were revealed as linear homogalacturonans interrupted by rhamnogalacturonan I in different lengths and extensities, where low esterification degrees (35.35-39.66%) were indicated via FT-IR.
31826397	2	23	theme	pectic	451:456	arg1	fractions					471:479	its fractions	467:479	its fractions (PFPP-10, -15 and -20)	467:502	According to composition analyses, pectic PFPP and its fractions (PFPP-10, -15 and -20) were revealed as linear homogalacturonans interrupted by rhamnogalacturonan I in different lengths and extensities, where low esterification degrees (35.35-39.66%) were indicated via FT-IR.
31826397	4	24	theme	coil	1102:1105	arg1	conformations					1107:1119	different semi-flexible coil conformations	1078:1119	different semi-flexible coil conformations	1078:1119	Therefore, different semi-flexible coil conformations were proposed schematically, where lower molecular-weight PFPP fractions were less flexible.
31826397	0	25	theme	Pectic	0:5	arg1	polysaccharides					7:21	Pectic polysaccharides	0:21	Pectic polysaccharides from purple passion fruit peel: A comprehensive study in macromolecular and conformational characterizations.	0:131	Pectic polysaccharides from purple passion fruit peel: A comprehensive study in macromolecular and conformational characterizations.
31826397	1	26	dep	%	242:242	arg1	w/w					245:247	w/w	245:247	w/w	245:247	A polysaccharide (PFPP) from purple passion fruit peel was optimally extracted, with the highest yield (10.05%, w/w) obtained under 35 °C extraction temperature, 240 W ultrasonic power, 65:1 mL/g liquid-to-solid ratio, 0.6% (w/v) ammonium oxalate, 30 min extraction time and pH 2.0.
31826397	0	27	theme	purple	28:33	arg1	peel					49:52	purple passion fruit peel	28:52	purple passion fruit peel	28:52	Pectic polysaccharides from purple passion fruit peel: A comprehensive study in macromolecular and conformational characterizations.
31826397	5	28	theme	food	1290:1293	arg1	industries					1314:1323	food and pharmaceutical industries	1290:1323	food and pharmaceutical industries	1290:1323	This could provide a molecular basis for precise re-utilizations of PFPP in food and pharmaceutical industries.
31826397	1	29	theme	%	355:355	arg1	oxalate					372:378	0.6% (w/v) ammonium oxalate	352:378	0.6% (w/v) ammonium oxalate	352:378	A polysaccharide (PFPP) from purple passion fruit peel was optimally extracted, with the highest yield (10.05%, w/w) obtained under 35 °C extraction temperature, 240 W ultrasonic power, 65:1 mL/g liquid-to-solid ratio, 0.6% (w/v) ammonium oxalate, 30 min extraction time and pH 2.0.
31826397	5	30	from	re-utilizations	1263:1277	arg1	industries					1314:1323	food and pharmaceutical industries	1290:1323	food and pharmaceutical industries	1290:1323	This could provide a molecular basis for precise re-utilizations of PFPP in food and pharmaceutical industries.
31826397	0	31	theme	fruit	43:47	arg1	peel					49:52	purple passion fruit peel	28:52	purple passion fruit peel	28:52	Pectic polysaccharides from purple passion fruit peel: A comprehensive study in macromolecular and conformational characterizations.
31826397	1	32	theme	35 °C	265:269	arg1	temperature					282:292	35 °C extraction temperature	265:292	35 °C extraction temperature	265:292	A polysaccharide (PFPP) from purple passion fruit peel was optimally extracted, with the highest yield (10.05%, w/w) obtained under 35 °C extraction temperature, 240 W ultrasonic power, 65:1 mL/g liquid-to-solid ratio, 0.6% (w/v) ammonium oxalate, 30 min extraction time and pH 2.0.
31826397	2	33	dep	fractions	471:479	arg1	-20					499:501	-20	499:501	-20	499:501	According to composition analyses, pectic PFPP and its fractions (PFPP-10, -15 and -20) were revealed as linear homogalacturonans interrupted by rhamnogalacturonan I in different lengths and extensities, where low esterification degrees (35.35-39.66%) were indicated via FT-IR.
31826397	2	33	dep	fractions	471:479	arg1	-15					491:493	-15	491:493	-15	491:493	According to composition analyses, pectic PFPP and its fractions (PFPP-10, -15 and -20) were revealed as linear homogalacturonans interrupted by rhamnogalacturonan I in different lengths and extensities, where low esterification degrees (35.35-39.66%) were indicated via FT-IR.
31826397	0	34	from	study	71:75	arg1	characterizations					114:130	macromolecular and conformational characterizations	80:130	macromolecular and conformational characterizations	80:130	Pectic polysaccharides from purple passion fruit peel: A comprehensive study in macromolecular and conformational characterizations.
31826397	5	35	theme	pharmaceutical	1299:1312	arg1	industries					1314:1323	food and pharmaceutical industries	1290:1323	food and pharmaceutical industries	1290:1323	This could provide a molecular basis for precise re-utilizations of PFPP in food and pharmaceutical industries.
31826397	0	36	theme	passion	35:41	arg1	peel					49:52	purple passion fruit peel	28:52	purple passion fruit peel	28:52	Pectic polysaccharides from purple passion fruit peel: A comprehensive study in macromolecular and conformational characterizations.
31826397	1	37	theme	extraction	271:280	arg1	temperature					282:292	35 °C extraction temperature	265:292	35 °C extraction temperature	265:292	A polysaccharide (PFPP) from purple passion fruit peel was optimally extracted, with the highest yield (10.05%, w/w) obtained under 35 °C extraction temperature, 240 W ultrasonic power, 65:1 mL/g liquid-to-solid ratio, 0.6% (w/v) ammonium oxalate, 30 min extraction time and pH 2.0.
31826397	4	38	theme	lower	1156:1160	arg1	fractions					1184:1192	lower molecular-weight PFPP fractions	1156:1192	lower molecular-weight PFPP fractions	1156:1192	Therefore, different semi-flexible coil conformations were proposed schematically, where lower molecular-weight PFPP fractions were less flexible.
31826397	3	39	theme	macromolecular	716:729	arg1	models					731:736	macromolecular models	716:736	macromolecular models	716:736	Furthermore, based on macromolecular models, comprehensive analyses on macromolecular and conformational characterizations of PFPP fractions were conducted quantitatively through, e.g., shape factor (1.42-1.79), Mark-Houwink-Sakurada exponent (0.55-0.74), conformational power-law exponent (0.52-0.58), fractal dimension (1.72-1.94) and persistence length (6.73-13.47 nm).
31826397	4	40	theme	different	1078:1086	arg1	conformations					1107:1119	different semi-flexible coil conformations	1078:1119	different semi-flexible coil conformations	1078:1119	Therefore, different semi-flexible coil conformations were proposed schematically, where lower molecular-weight PFPP fractions were less flexible.
31826397	4	41	theme	molecular-weight	1162:1177	arg1	fractions					1184:1192	lower molecular-weight PFPP fractions	1156:1192	lower molecular-weight PFPP fractions	1156:1192	Therefore, different semi-flexible coil conformations were proposed schematically, where lower molecular-weight PFPP fractions were less flexible.
31826397	1	42	theme	ammonium	363:370	arg1	oxalate					372:378	0.6% (w/v) ammonium oxalate	352:378	0.6% (w/v) ammonium oxalate	352:378	A polysaccharide (PFPP) from purple passion fruit peel was optimally extracted, with the highest yield (10.05%, w/w) obtained under 35 °C extraction temperature, 240 W ultrasonic power, 65:1 mL/g liquid-to-solid ratio, 0.6% (w/v) ammonium oxalate, 30 min extraction time and pH 2.0.
31826397	4	43	theme	PFPP	1179:1182	arg1	fractions					1184:1192	lower molecular-weight PFPP fractions	1156:1192	lower molecular-weight PFPP fractions	1156:1192	Therefore, different semi-flexible coil conformations were proposed schematically, where lower molecular-weight PFPP fractions were less flexible.
31826397	3	44	theme	PFPP	820:823	arg1	fractions					825:833	PFPP fractions	820:833	PFPP fractions	820:833	Furthermore, based on macromolecular models, comprehensive analyses on macromolecular and conformational characterizations of PFPP fractions were conducted quantitatively through, e.g., shape factor (1.42-1.79), Mark-Houwink-Sakurada exponent (0.55-0.74), conformational power-law exponent (0.52-0.58), fractal dimension (1.72-1.94) and persistence length (6.73-13.47 nm).
31826397	1	45	theme	240 W	295:299	arg1	power					312:316	240 W ultrasonic power	295:316	240 W ultrasonic power	295:316	A polysaccharide (PFPP) from purple passion fruit peel was optimally extracted, with the highest yield (10.05%, w/w) obtained under 35 °C extraction temperature, 240 W ultrasonic power, 65:1 mL/g liquid-to-solid ratio, 0.6% (w/v) ammonium oxalate, 30 min extraction time and pH 2.0.
31826397	3	46	theme	fractal	997:1003	arg1	1.72-1.94					1016:1024	1.72-1.94	1016:1024	1.72-1.94	1016:1024	Furthermore, based on macromolecular models, comprehensive analyses on macromolecular and conformational characterizations of PFPP fractions were conducted quantitatively through, e.g., shape factor (1.42-1.79), Mark-Houwink-Sakurada exponent (0.55-0.74), conformational power-law exponent (0.52-0.58), fractal dimension (1.72-1.94) and persistence length (6.73-13.47 nm).
31826397	3	46	theme	fractal	997:1003	arg1	factor					886:891	shape factor	880:891	shape factor (1.42-1.79)	880:903	Furthermore, based on macromolecular models, comprehensive analyses on macromolecular and conformational characterizations of PFPP fractions were conducted quantitatively through, e.g., shape factor (1.42-1.79), Mark-Houwink-Sakurada exponent (0.55-0.74), conformational power-law exponent (0.52-0.58), fractal dimension (1.72-1.94) and persistence length (6.73-13.47 nm).
31826397	3	46	theme	fractal	997:1003	arg1	dimension					1005:1013	fractal dimension	997:1013	fractal dimension (1.72-1.94)	997:1025	Furthermore, based on macromolecular models, comprehensive analyses on macromolecular and conformational characterizations of PFPP fractions were conducted quantitatively through, e.g., shape factor (1.42-1.79), Mark-Houwink-Sakurada exponent (0.55-0.74), conformational power-law exponent (0.52-0.58), fractal dimension (1.72-1.94) and persistence length (6.73-13.47 nm).
31826397	2	47	theme	esterification	630:643	arg1	degrees					645:651	low esterification degrees	626:651	low esterification degrees (35.35-39.66%)	626:666	According to composition analyses, pectic PFPP and its fractions (PFPP-10, -15 and -20) were revealed as linear homogalacturonans interrupted by rhamnogalacturonan I in different lengths and extensities, where low esterification degrees (35.35-39.66%) were indicated via FT-IR.
31826397	2	47	theme	esterification	630:643	arg1	%					665:665	35.35-39.66%	654:665	35.35-39.66%	654:665	According to composition analyses, pectic PFPP and its fractions (PFPP-10, -15 and -20) were revealed as linear homogalacturonans interrupted by rhamnogalacturonan I in different lengths and extensities, where low esterification degrees (35.35-39.66%) were indicated via FT-IR.
31826397	3	48	theme	fractions	825:833	arg1	analyses					753:760	comprehensive analyses	739:760	comprehensive analyses on macromolecular and conformational characterizations of PFPP fractions	739:833	Furthermore, based on macromolecular models, comprehensive analyses on macromolecular and conformational characterizations of PFPP fractions were conducted quantitatively through, e.g., shape factor (1.42-1.79), Mark-Houwink-Sakurada exponent (0.55-0.74), conformational power-law exponent (0.52-0.58), fractal dimension (1.72-1.94) and persistence length (6.73-13.47 nm).
31826397	3	49	theme	comprehensive	739:751	arg1	analyses					753:760	comprehensive analyses	739:760	comprehensive analyses on macromolecular and conformational characterizations of PFPP fractions	739:833	Furthermore, based on macromolecular models, comprehensive analyses on macromolecular and conformational characterizations of PFPP fractions were conducted quantitatively through, e.g., shape factor (1.42-1.79), Mark-Houwink-Sakurada exponent (0.55-0.74), conformational power-law exponent (0.52-0.58), fractal dimension (1.72-1.94) and persistence length (6.73-13.47 nm).
31826397	3	50	theme	Mark-Houwink-Sakurada	906:926	arg1	exponent					928:935	Mark-Houwink-Sakurada exponent	906:935	Mark-Houwink-Sakurada exponent (0.55-0.74)	906:947	Furthermore, based on macromolecular models, comprehensive analyses on macromolecular and conformational characterizations of PFPP fractions were conducted quantitatively through, e.g., shape factor (1.42-1.79), Mark-Houwink-Sakurada exponent (0.55-0.74), conformational power-law exponent (0.52-0.58), fractal dimension (1.72-1.94) and persistence length (6.73-13.47 nm).
31826397	3	50	theme	Mark-Houwink-Sakurada	906:926	arg1	factor					886:891	shape factor	880:891	shape factor (1.42-1.79)	880:903	Furthermore, based on macromolecular models, comprehensive analyses on macromolecular and conformational characterizations of PFPP fractions were conducted quantitatively through, e.g., shape factor (1.42-1.79), Mark-Houwink-Sakurada exponent (0.55-0.74), conformational power-law exponent (0.52-0.58), fractal dimension (1.72-1.94) and persistence length (6.73-13.47 nm).
31826397	3	50	theme	Mark-Houwink-Sakurada	906:926	arg1	0.55-0.74					938:946	0.55-0.74	938:946	0.55-0.74	938:946	Furthermore, based on macromolecular models, comprehensive analyses on macromolecular and conformational characterizations of PFPP fractions were conducted quantitatively through, e.g., shape factor (1.42-1.79), Mark-Houwink-Sakurada exponent (0.55-0.74), conformational power-law exponent (0.52-0.58), fractal dimension (1.72-1.94) and persistence length (6.73-13.47 nm).
31826397	3	50	theme	Mark-Houwink-Sakurada	906:926	arg1	6.73-13.47 nm					1051:1063	6.73-13.47 nm	1051:1063	6.73-13.47 nm	1051:1063	Furthermore, based on macromolecular models, comprehensive analyses on macromolecular and conformational characterizations of PFPP fractions were conducted quantitatively through, e.g., shape factor (1.42-1.79), Mark-Houwink-Sakurada exponent (0.55-0.74), conformational power-law exponent (0.52-0.58), fractal dimension (1.72-1.94) and persistence length (6.73-13.47 nm).
31826397	1	51	from	peel	183:186	arg1	polysaccharide					135:148	A polysaccharide	133:148	A polysaccharide (PFPP) from purple passion fruit peel	133:186	A polysaccharide (PFPP) from purple passion fruit peel was optimally extracted, with the highest yield (10.05%, w/w) obtained under 35 °C extraction temperature, 240 W ultrasonic power, 65:1 mL/g liquid-to-solid ratio, 0.6% (w/v) ammonium oxalate, 30 min extraction time and pH 2.0.
31826397	1	51	from	peel	183:186	arg1	PFPP					151:154	PFPP	151:154	PFPP	151:154	A polysaccharide (PFPP) from purple passion fruit peel was optimally extracted, with the highest yield (10.05%, w/w) obtained under 35 °C extraction temperature, 240 W ultrasonic power, 65:1 mL/g liquid-to-solid ratio, 0.6% (w/v) ammonium oxalate, 30 min extraction time and pH 2.0.
31826397	1	52	theme	highest	222:228	arg1	yield					230:234	the highest yield	218:234	the highest yield (10.05%, w/w) obtained under 35 °C extraction temperature, 240 W ultrasonic power, 65:1 mL/g liquid-to-solid ratio, 0.6% (w/v) ammonium oxalate, 30 min extraction time and pH 2.0	218:413	A polysaccharide (PFPP) from purple passion fruit peel was optimally extracted, with the highest yield (10.05%, w/w) obtained under 35 °C extraction temperature, 240 W ultrasonic power, 65:1 mL/g liquid-to-solid ratio, 0.6% (w/v) ammonium oxalate, 30 min extraction time and pH 2.0.
31826397	1	52	theme	highest	222:228	arg1	%					242:242	10.05%	237:242	10.05%	237:242	A polysaccharide (PFPP) from purple passion fruit peel was optimally extracted, with the highest yield (10.05%, w/w) obtained under 35 °C extraction temperature, 240 W ultrasonic power, 65:1 mL/g liquid-to-solid ratio, 0.6% (w/v) ammonium oxalate, 30 min extraction time and pH 2.0.
31826397	0	53	theme	comprehensive	57:69	arg1	study					71:75	A comprehensive study	55:75	Pectic polysaccharides from purple passion fruit peel: A comprehensive study in macromolecular and conformational characterizations.	0:131	Pectic polysaccharides from purple passion fruit peel: A comprehensive study in macromolecular and conformational characterizations.
31826397	1	54	theme	30 min	381:386	arg1	time					399:402	30 min extraction time	381:402	30 min extraction time	381:402	A polysaccharide (PFPP) from purple passion fruit peel was optimally extracted, with the highest yield (10.05%, w/w) obtained under 35 °C extraction temperature, 240 W ultrasonic power, 65:1 mL/g liquid-to-solid ratio, 0.6% (w/v) ammonium oxalate, 30 min extraction time and pH 2.0.
31826397	0	55	dep	polysaccharides	7:21	arg1	study					71:75	A comprehensive study	55:75	Pectic polysaccharides from purple passion fruit peel: A comprehensive study in macromolecular and conformational characterizations.	0:131	Pectic polysaccharides from purple passion fruit peel: A comprehensive study in macromolecular and conformational characterizations.
31826397	3	56	theme	conformational	950:963	arg1	0.52-0.58					985:993	0.52-0.58	985:993	0.52-0.58	985:993	Furthermore, based on macromolecular models, comprehensive analyses on macromolecular and conformational characterizations of PFPP fractions were conducted quantitatively through, e.g., shape factor (1.42-1.79), Mark-Houwink-Sakurada exponent (0.55-0.74), conformational power-law exponent (0.52-0.58), fractal dimension (1.72-1.94) and persistence length (6.73-13.47 nm).
31826397	3	56	theme	conformational	950:963	arg1	exponent					975:982	conformational power-law exponent	950:982	conformational power-law exponent (0.52-0.58)	950:994	Furthermore, based on macromolecular models, comprehensive analyses on macromolecular and conformational characterizations of PFPP fractions were conducted quantitatively through, e.g., shape factor (1.42-1.79), Mark-Houwink-Sakurada exponent (0.55-0.74), conformational power-law exponent (0.52-0.58), fractal dimension (1.72-1.94) and persistence length (6.73-13.47 nm).
31826397	3	56	theme	conformational	950:963	arg1	factor					886:891	shape factor	880:891	shape factor (1.42-1.79)	880:903	Furthermore, based on macromolecular models, comprehensive analyses on macromolecular and conformational characterizations of PFPP fractions were conducted quantitatively through, e.g., shape factor (1.42-1.79), Mark-Houwink-Sakurada exponent (0.55-0.74), conformational power-law exponent (0.52-0.58), fractal dimension (1.72-1.94) and persistence length (6.73-13.47 nm).
31826397	5	57	theme	PFPP	1282:1285	arg1	re-utilizations					1263:1277	precise re-utilizations	1255:1277	precise re-utilizations of PFPP in food and pharmaceutical industries	1255:1323	This could provide a molecular basis for precise re-utilizations of PFPP in food and pharmaceutical industries.
31826397	1	58	dep	%	355:355	arg1	w/v					358:360	w/v	358:360	w/v	358:360	A polysaccharide (PFPP) from purple passion fruit peel was optimally extracted, with the highest yield (10.05%, w/w) obtained under 35 °C extraction temperature, 240 W ultrasonic power, 65:1 mL/g liquid-to-solid ratio, 0.6% (w/v) ammonium oxalate, 30 min extraction time and pH 2.0.
31826397	1	59	theme	ultrasonic	301:310	arg1	power					312:316	240 W ultrasonic power	295:316	240 W ultrasonic power	295:316	A polysaccharide (PFPP) from purple passion fruit peel was optimally extracted, with the highest yield (10.05%, w/w) obtained under 35 °C extraction temperature, 240 W ultrasonic power, 65:1 mL/g liquid-to-solid ratio, 0.6% (w/v) ammonium oxalate, 30 min extraction time and pH 2.0.
29559311	8	0	theme	OGT	1058:1060	arg1	activity					1062:1069	OGT activity	1058:1069	OGT activity	1058:1069	Instead, the six OPFRs substituted with aromatic or chlorinated alkyl groups inhibited OGT activity significantly, with tri-m-cresyl phosphate (TCrP) being the strongest.
29559311	0	1	link	O-linked	14:21	arg1	transferase					43:53	O-linked N-acetylglucosamine transferase	14:53	O-linked N-acetylglucosamine transferase activity	14:62	Inhibition of O-linked N-acetylglucosamine transferase activity in PC12 cells - A molecular mechanism of organophosphate flame retardants developmental neurotoxicity.
29559311	4	2	theme	developmental	662:674	arg1	neurotoxicity					676:688	the developmental neurotoxicity	658:688	the developmental neurotoxicity of OPFRs	658:697	In this study, we aimed to investigate the molecular mechanisms accounting for the developmental neurotoxicity of OPFRs by identifying potential targets of OPFRs and the attendant effects.
29559311	11	3	theme	complexes	1490:1498	arg1	docking					1466:1472	Molecular docking	1456:1472	Molecular docking of the OGT/OPFR complexes	1456:1498	Molecular docking of the OGT/OPFR complexes provided rationales for the difference in their structure-dependent inhibition potency.
29559311	7	4	theme	inhibitory	952:961	arg1	effect					963:968	no inhibitory effect	949:968	no inhibitory effect	949:968	The alkyl group substituted OPFRs had no inhibitory effect.
29559311	4	5	theme	attendant	749:757	arg1	effects					759:765	the attendant effects	745:765	the attendant effects	745:765	In this study, we aimed to investigate the molecular mechanisms accounting for the developmental neurotoxicity of OPFRs by identifying potential targets of OPFRs and the attendant effects.
29559311	2	6	theme	molecular	346:354	arg1	mechanisms					356:365	the molecular mechanisms	342:365	the molecular mechanisms underlying the toxicity	342:389	However, the molecular mechanisms underlying the toxicity remain elusive.
29559311	7	7	theme	substituted	927:937	arg1	OPFRs					939:943	substituted OPFRs	927:943	The alkyl group substituted OPFRs	911:943	The alkyl group substituted OPFRs had no inhibitory effect.
29559311	4	8	theme	potential	714:722	arg1	targets					724:730	potential targets	714:730	potential targets of OPFRs and the attendant effects	714:765	In this study, we aimed to investigate the molecular mechanisms accounting for the developmental neurotoxicity of OPFRs by identifying potential targets of OPFRs and the attendant effects.
29559311	0	9	theme	retardants	127:136	arg1	mechanism					92:100	A molecular mechanism	80:100	Inhibition of O-linked N-acetylglucosamine transferase activity in PC12 cells - A molecular mechanism of organophosphate flame retardants developmental neurotoxicity.	0:165	Inhibition of O-linked N-acetylglucosamine transferase activity in PC12 cells - A molecular mechanism of organophosphate flame retardants developmental neurotoxicity.
29559311	7	10	theme	alkyl	915:919	arg1	group					921:925	The alkyl group substituted OPFRs	911:943	The alkyl group substituted OPFRs	911:943	The alkyl group substituted OPFRs had no inhibitory effect.
29559311	12	11	theme	major	1700:1704	arg1	target					1630:1635	a new biological target	1613:1635	a new biological target	1613:1635	Our findings may provide a new biological target of OPFRs in their neurotoxicological actions, which might be a major molecular mechanism of OPFRs developmental neurotoxicity.
29559311	12	11	theme	major	1700:1704	arg1	mechanism					1716:1724	a major molecular mechanism	1698:1724	a major molecular mechanism of OPFRs developmental neurotoxicity	1698:1761	Our findings may provide a new biological target of OPFRs in their neurotoxicological actions, which might be a major molecular mechanism of OPFRs developmental neurotoxicity.
29559311	1	12	theme	flame	239:243	arg1	retardants					245:254	brominated flame retardants	228:254	brominated flame retardants	228:254	Organophosphate flame retardants (OPFRs), as alternatives of brominated flame retardants, can cause neurodevelopmental effects similar to organophosphate pesticides.
29559311	10	13	theme	subsequent	1315:1324	arg1	species					1381:1387	intracellular reactive oxygen species	1351:1387	intracellular reactive oxygen species (ROS)	1351:1393	Inhibition of OGT by OPFRs might be involved in the subsequent toxic effects, including intracellular reactive oxygen species (ROS), calcium level, as well as cell proliferation and autophagy.
29559311	10	13	theme	subsequent	1315:1324	arg1	level					1404:1408	calcium level	1396:1408	calcium level	1396:1408	Inhibition of OGT by OPFRs might be involved in the subsequent toxic effects, including intracellular reactive oxygen species (ROS), calcium level, as well as cell proliferation and autophagy.
29559311	10	13	theme	subsequent	1315:1324	arg1	proliferation					1427:1439	cell proliferation	1422:1439	cell proliferation	1422:1439	Inhibition of OGT by OPFRs might be involved in the subsequent toxic effects, including intracellular reactive oxygen species (ROS), calcium level, as well as cell proliferation and autophagy.
29559311	10	13	theme	subsequent	1315:1324	arg1	effects					1332:1338	the subsequent toxic effects	1311:1338	the subsequent toxic effects	1311:1338	Inhibition of OGT by OPFRs might be involved in the subsequent toxic effects, including intracellular reactive oxygen species (ROS), calcium level, as well as cell proliferation and autophagy.
29559311	9	14	theme	OGT	1191:1193	arg1	activity					1195:1202	OGT activity	1191:1202	OGT activity in PC12 cells	1191:1216	The six OPFRs (0-100 μM exposure) also inhibited OGT activity in PC12 cells and decreased protein O-GlcNAcylation level.
29559311	12	15	theme	OPFRs	1640:1644	arg1	target					1630:1635	a new biological target	1613:1635	a new biological target	1613:1635	Our findings may provide a new biological target of OPFRs in their neurotoxicological actions, which might be a major molecular mechanism of OPFRs developmental neurotoxicity.
29559311	12	15	theme	OPFRs	1640:1644	arg1	mechanism					1716:1724	a major molecular mechanism	1698:1724	a major molecular mechanism of OPFRs developmental neurotoxicity	1698:1761	Our findings may provide a new biological target of OPFRs in their neurotoxicological actions, which might be a major molecular mechanism of OPFRs developmental neurotoxicity.
29559311	5	16	theme	electrochemical	836:850	arg1	biosensor					852:860	an electrochemical biosensor	833:860	an electrochemical biosensor	833:860	Twelve OPFRs were evaluated for inhibition of OGT activity using an electrochemical biosensor.
29559311	1	17	theme	flame	183:187	arg1	OPFRs					201:205	OPFRs	201:205	OPFRs	201:205	Organophosphate flame retardants (OPFRs), as alternatives of brominated flame retardants, can cause neurodevelopmental effects similar to organophosphate pesticides.
29559311	1	17	theme	flame	183:187	arg1	retardants					189:198	Organophosphate flame retardants	167:198	Organophosphate flame retardants (OPFRs)	167:206	Organophosphate flame retardants (OPFRs), as alternatives of brominated flame retardants, can cause neurodevelopmental effects similar to organophosphate pesticides.
29559311	0	18	dep	retardants	127:136	arg1	neurotoxicity					152:164	developmental neurotoxicity	138:164	organophosphate flame retardants developmental neurotoxicity	105:164	Inhibition of O-linked N-acetylglucosamine transferase activity in PC12 cells - A molecular mechanism of organophosphate flame retardants developmental neurotoxicity.
29559311	12	19	theme	new	1615:1617	arg1	target					1630:1635	a new biological target	1613:1635	a new biological target	1613:1635	Our findings may provide a new biological target of OPFRs in their neurotoxicological actions, which might be a major molecular mechanism of OPFRs developmental neurotoxicity.
29559311	12	19	theme	new	1615:1617	arg1	mechanism					1716:1724	a major molecular mechanism	1698:1724	a major molecular mechanism of OPFRs developmental neurotoxicity	1698:1761	Our findings may provide a new biological target of OPFRs in their neurotoxicological actions, which might be a major molecular mechanism of OPFRs developmental neurotoxicity.
29559311	3	20	theme	O-linked	407:414	arg1	O-GlcNAc					437:444	O-GlcNAc	437:444	O-GlcNAc	437:444	O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) regulates numerous neural processes through the O-GlcNAcylation modification of nuclear and cytoplasmic proteins.
29559311	3	20	theme	O-linked	407:414	arg1	N-acetylglucosamine					416:434	O-linked N-acetylglucosamine	407:434	O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT)	407:463	O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) regulates numerous neural processes through the O-GlcNAcylation modification of nuclear and cytoplasmic proteins.
29559311	0	21	from	Inhibition	0:9	arg1	cells					72:76	PC12 cells	67:76	PC12 cells	67:76	Inhibition of O-linked N-acetylglucosamine transferase activity in PC12 cells - A molecular mechanism of organophosphate flame retardants developmental neurotoxicity.
29559311	5	22	theme	OGT	814:816	arg1	activity					818:825	OGT activity	814:825	OGT activity	814:825	Twelve OPFRs were evaluated for inhibition of OGT activity using an electrochemical biosensor.
29559311	12	23	from	OPFRs	1640:1644	arg1	actions					1674:1680	their neurotoxicological actions	1649:1680	their neurotoxicological actions	1649:1680	Our findings may provide a new biological target of OPFRs in their neurotoxicological actions, which might be a major molecular mechanism of OPFRs developmental neurotoxicity.
29559311	3	24	mod	modification	529:540	arg1	proteins					569:576	nuclear and cytoplasmic proteins	545:576	nuclear and cytoplasmic proteins	545:576	O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) regulates numerous neural processes through the O-GlcNAcylation modification of nuclear and cytoplasmic proteins.
29559311	3	24	mod	modification	529:540	arg3	O-GlcNAcylation					513:527	the O-GlcNAcylation modification	509:540	the O-GlcNAcylation modification of nuclear and cytoplasmic proteins	509:576	O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) regulates numerous neural processes through the O-GlcNAcylation modification of nuclear and cytoplasmic proteins.
29559311	0	25	theme	organophosphate	105:119	arg1	retardants					127:136	organophosphate flame retardants developmental neurotoxicity	105:164	organophosphate flame retardants developmental neurotoxicity	105:164	Inhibition of O-linked N-acetylglucosamine transferase activity in PC12 cells - A molecular mechanism of organophosphate flame retardants developmental neurotoxicity.
29559311	12	26	from	target	1630:1635	arg1	actions					1674:1680	their neurotoxicological actions	1649:1680	their neurotoxicological actions	1649:1680	Our findings may provide a new biological target of OPFRs in their neurotoxicological actions, which might be a major molecular mechanism of OPFRs developmental neurotoxicity.
29559311	10	27	theme	reactive	1365:1372	arg1	species					1381:1387	intracellular reactive oxygen species	1351:1387	intracellular reactive oxygen species (ROS)	1351:1393	Inhibition of OGT by OPFRs might be involved in the subsequent toxic effects, including intracellular reactive oxygen species (ROS), calcium level, as well as cell proliferation and autophagy.
29559311	10	27	theme	reactive	1365:1372	arg1	ROS					1390:1392	ROS	1390:1392	ROS	1390:1392	Inhibition of OGT by OPFRs might be involved in the subsequent toxic effects, including intracellular reactive oxygen species (ROS), calcium level, as well as cell proliferation and autophagy.
29559311	3	28	theme	numerous	475:482	arg1	processes					491:499	numerous neural processes	475:499	numerous neural processes	475:499	O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) regulates numerous neural processes through the O-GlcNAcylation modification of nuclear and cytoplasmic proteins.
29559311	1	29	theme	organophosphate	305:319	arg1	pesticides					321:330	organophosphate pesticides	305:330	organophosphate pesticides	305:330	Organophosphate flame retardants (OPFRs), as alternatives of brominated flame retardants, can cause neurodevelopmental effects similar to organophosphate pesticides.
29559311	11	30	theme	structure-dependent	1548:1566	arg1	potency					1579:1585	their structure-dependent inhibition potency	1542:1585	their structure-dependent inhibition potency	1542:1585	Molecular docking of the OGT/OPFR complexes provided rationales for the difference in their structure-dependent inhibition potency.
29559311	0	31	theme	O-linked	14:21	arg1	transferase					43:53	O-linked N-acetylglucosamine transferase	14:53	O-linked N-acetylglucosamine transferase activity	14:62	Inhibition of O-linked N-acetylglucosamine transferase activity in PC12 cells - A molecular mechanism of organophosphate flame retardants developmental neurotoxicity.
29559311	12	32	theme	developmental	1735:1747	arg1	neurotoxicity					1749:1761	OPFRs developmental neurotoxicity	1729:1761	OPFRs developmental neurotoxicity	1729:1761	Our findings may provide a new biological target of OPFRs in their neurotoxicological actions, which might be a major molecular mechanism of OPFRs developmental neurotoxicity.
29559311	4	33	theme	molecular	622:630	arg1	mechanisms					632:641	the molecular mechanisms	618:641	the molecular mechanisms accounting for the developmental neurotoxicity of OPFRs	618:697	In this study, we aimed to investigate the molecular mechanisms accounting for the developmental neurotoxicity of OPFRs by identifying potential targets of OPFRs and the attendant effects.
29559311	9	34	from	activity	1195:1202	arg1	cells					1212:1216	PC12 cells	1207:1216	PC12 cells	1207:1216	The six OPFRs (0-100 μM exposure) also inhibited OGT activity in PC12 cells and decreased protein O-GlcNAcylation level.
29559311	0	35	theme	transferase	43:53	arg1	activity					55:62	O-linked N-acetylglucosamine transferase activity	14:62	O-linked N-acetylglucosamine transferase activity	14:62	Inhibition of O-linked N-acetylglucosamine transferase activity in PC12 cells - A molecular mechanism of organophosphate flame retardants developmental neurotoxicity.
29559311	3	36	theme	cytoplasmic	557:567	arg1	proteins					569:576	nuclear and cytoplasmic proteins	545:576	nuclear and cytoplasmic proteins	545:576	O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) regulates numerous neural processes through the O-GlcNAcylation modification of nuclear and cytoplasmic proteins.
29559311	8	37	theme	alkyl	1035:1039	arg1	groups					1041:1046	aromatic or chlorinated alkyl groups	1011:1046	aromatic or chlorinated alkyl groups	1011:1046	Instead, the six OPFRs substituted with aromatic or chlorinated alkyl groups inhibited OGT activity significantly, with tri-m-cresyl phosphate (TCrP) being the strongest.
29559311	3	38	theme	nuclear	545:551	arg1	proteins					569:576	nuclear and cytoplasmic proteins	545:576	nuclear and cytoplasmic proteins	545:576	O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) regulates numerous neural processes through the O-GlcNAcylation modification of nuclear and cytoplasmic proteins.
29559311	1	39	theme	retardants	245:254	arg1	alternatives					212:223	alternatives	212:223	alternatives of brominated flame retardants	212:254	Organophosphate flame retardants (OPFRs), as alternatives of brominated flame retardants, can cause neurodevelopmental effects similar to organophosphate pesticides.
29559311	12	40	from	actions	1674:1680	arg1	target					1630:1635	a new biological target	1613:1635	a new biological target	1613:1635	Our findings may provide a new biological target of OPFRs in their neurotoxicological actions, which might be a major molecular mechanism of OPFRs developmental neurotoxicity.
29559311	12	40	from	actions	1674:1680	arg1	mechanism					1716:1724	a major molecular mechanism	1698:1724	a major molecular mechanism of OPFRs developmental neurotoxicity	1698:1761	Our findings may provide a new biological target of OPFRs in their neurotoxicological actions, which might be a major molecular mechanism of OPFRs developmental neurotoxicity.
29559311	11	41	theme	Molecular	1456:1464	arg1	docking					1466:1472	Molecular docking	1456:1472	Molecular docking of the OGT/OPFR complexes	1456:1498	Molecular docking of the OGT/OPFR complexes provided rationales for the difference in their structure-dependent inhibition potency.
29559311	9	42	theme	O-GlcNAcylation	1240:1254	arg1	level					1256:1260	protein O-GlcNAcylation level	1232:1260	protein O-GlcNAcylation level	1232:1260	The six OPFRs (0-100 μM exposure) also inhibited OGT activity in PC12 cells and decreased protein O-GlcNAcylation level.
29559311	0	43	dep	Inhibition	0:9	arg1	mechanism					92:100	A molecular mechanism	80:100	Inhibition of O-linked N-acetylglucosamine transferase activity in PC12 cells - A molecular mechanism of organophosphate flame retardants developmental neurotoxicity.	0:165	Inhibition of O-linked N-acetylglucosamine transferase activity in PC12 cells - A molecular mechanism of organophosphate flame retardants developmental neurotoxicity.
29559311	4	44	theme	OPFRs	693:697	arg1	neurotoxicity					676:688	the developmental neurotoxicity	658:688	the developmental neurotoxicity of OPFRs	658:697	In this study, we aimed to investigate the molecular mechanisms accounting for the developmental neurotoxicity of OPFRs by identifying potential targets of OPFRs and the attendant effects.
29559311	1	45	theme	neurodevelopmental	267:284	arg1	effects					286:292	neurodevelopmental effects	267:292	neurodevelopmental effects similar to organophosphate pesticides	267:330	Organophosphate flame retardants (OPFRs), as alternatives of brominated flame retardants, can cause neurodevelopmental effects similar to organophosphate pesticides.
29559311	11	46	theme	OGT/OPFR	1481:1488	arg1	complexes					1490:1498	the OGT/OPFR complexes	1477:1498	the OGT/OPFR complexes	1477:1498	Molecular docking of the OGT/OPFR complexes provided rationales for the difference in their structure-dependent inhibition potency.
29559311	1	47	theme	similar	294:300	arg1	effects					286:292	neurodevelopmental effects	267:292	neurodevelopmental effects similar to organophosphate pesticides	267:330	Organophosphate flame retardants (OPFRs), as alternatives of brominated flame retardants, can cause neurodevelopmental effects similar to organophosphate pesticides.
29559311	8	48	theme	tri-m-cresyl	1091:1102	arg1	strongest					1131:1139	strongest	1131:1139	strongest	1131:1139	Instead, the six OPFRs substituted with aromatic or chlorinated alkyl groups inhibited OGT activity significantly, with tri-m-cresyl phosphate (TCrP) being the strongest.
29559311	8	48	theme	tri-m-cresyl	1091:1102	arg1	TCrP					1115:1118	TCrP	1115:1118	TCrP	1115:1118	Instead, the six OPFRs substituted with aromatic or chlorinated alkyl groups inhibited OGT activity significantly, with tri-m-cresyl phosphate (TCrP) being the strongest.
29559311	8	48	theme	tri-m-cresyl	1091:1102	arg1	phosphate					1104:1112	tri-m-cresyl phosphate	1091:1112	tri-m-cresyl phosphate (TCrP)	1091:1119	Instead, the six OPFRs substituted with aromatic or chlorinated alkyl groups inhibited OGT activity significantly, with tri-m-cresyl phosphate (TCrP) being the strongest.
29559311	6	49	theme	substituent	891:901	arg1	groups					903:908	substituent groups	891:908	substituent groups	891:908	Their potency differed with substituent groups.
29559311	3	50	link	O-linked	407:414	arg1	O-GlcNAc					437:444	O-GlcNAc	437:444	O-GlcNAc	437:444	O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) regulates numerous neural processes through the O-GlcNAcylation modification of nuclear and cytoplasmic proteins.
29559311	3	50	link	O-linked	407:414	arg1	N-acetylglucosamine					416:434	O-linked N-acetylglucosamine	407:434	O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT)	407:463	O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) regulates numerous neural processes through the O-GlcNAcylation modification of nuclear and cytoplasmic proteins.
29559311	4	51	theme	OPFRs	735:739	arg1	targets					724:730	potential targets	714:730	potential targets of OPFRs and the attendant effects	714:765	In this study, we aimed to investigate the molecular mechanisms accounting for the developmental neurotoxicity of OPFRs by identifying potential targets of OPFRs and the attendant effects.
29559311	0	52	theme	developmental	138:150	arg1	neurotoxicity					152:164	developmental neurotoxicity	138:164	organophosphate flame retardants developmental neurotoxicity	105:164	Inhibition of O-linked N-acetylglucosamine transferase activity in PC12 cells - A molecular mechanism of organophosphate flame retardants developmental neurotoxicity.
29559311	12	53	theme	molecular	1706:1714	arg1	target					1630:1635	a new biological target	1613:1635	a new biological target	1613:1635	Our findings may provide a new biological target of OPFRs in their neurotoxicological actions, which might be a major molecular mechanism of OPFRs developmental neurotoxicity.
29559311	12	53	theme	molecular	1706:1714	arg1	mechanism					1716:1724	a major molecular mechanism	1698:1724	a major molecular mechanism of OPFRs developmental neurotoxicity	1698:1761	Our findings may provide a new biological target of OPFRs in their neurotoxicological actions, which might be a major molecular mechanism of OPFRs developmental neurotoxicity.
29559311	1	54	theme	brominated	228:237	arg1	retardants					245:254	brominated flame retardants	228:254	brominated flame retardants	228:254	Organophosphate flame retardants (OPFRs), as alternatives of brominated flame retardants, can cause neurodevelopmental effects similar to organophosphate pesticides.
29559311	10	55	theme	toxic	1326:1330	arg1	species					1381:1387	intracellular reactive oxygen species	1351:1387	intracellular reactive oxygen species (ROS)	1351:1393	Inhibition of OGT by OPFRs might be involved in the subsequent toxic effects, including intracellular reactive oxygen species (ROS), calcium level, as well as cell proliferation and autophagy.
29559311	10	55	theme	toxic	1326:1330	arg1	level					1404:1408	calcium level	1396:1408	calcium level	1396:1408	Inhibition of OGT by OPFRs might be involved in the subsequent toxic effects, including intracellular reactive oxygen species (ROS), calcium level, as well as cell proliferation and autophagy.
29559311	10	55	theme	toxic	1326:1330	arg1	proliferation					1427:1439	cell proliferation	1422:1439	cell proliferation	1422:1439	Inhibition of OGT by OPFRs might be involved in the subsequent toxic effects, including intracellular reactive oxygen species (ROS), calcium level, as well as cell proliferation and autophagy.
29559311	10	55	theme	toxic	1326:1330	arg1	effects					1332:1338	the subsequent toxic effects	1311:1338	the subsequent toxic effects	1311:1338	Inhibition of OGT by OPFRs might be involved in the subsequent toxic effects, including intracellular reactive oxygen species (ROS), calcium level, as well as cell proliferation and autophagy.
29559311	11	56	theme	inhibition	1568:1577	arg1	potency					1579:1585	their structure-dependent inhibition potency	1542:1585	their structure-dependent inhibition potency	1542:1585	Molecular docking of the OGT/OPFR complexes provided rationales for the difference in their structure-dependent inhibition potency.
29559311	1	57	theme	Organophosphate	167:181	arg1	OPFRs					201:205	OPFRs	201:205	OPFRs	201:205	Organophosphate flame retardants (OPFRs), as alternatives of brominated flame retardants, can cause neurodevelopmental effects similar to organophosphate pesticides.
29559311	1	57	theme	Organophosphate	167:181	arg1	retardants					189:198	Organophosphate flame retardants	167:198	Organophosphate flame retardants (OPFRs)	167:206	Organophosphate flame retardants (OPFRs), as alternatives of brominated flame retardants, can cause neurodevelopmental effects similar to organophosphate pesticides.
29559311	12	58	theme	neurotoxicological	1655:1672	arg1	actions					1674:1680	their neurotoxicological actions	1649:1680	their neurotoxicological actions	1649:1680	Our findings may provide a new biological target of OPFRs in their neurotoxicological actions, which might be a major molecular mechanism of OPFRs developmental neurotoxicity.
29559311	4	59	theme	effects	759:765	arg1	targets					724:730	potential targets	714:730	potential targets of OPFRs and the attendant effects	714:765	In this study, we aimed to investigate the molecular mechanisms accounting for the developmental neurotoxicity of OPFRs by identifying potential targets of OPFRs and the attendant effects.
29559311	10	60	theme	intracellular	1351:1363	arg1	species					1381:1387	intracellular reactive oxygen species	1351:1387	intracellular reactive oxygen species (ROS)	1351:1393	Inhibition of OGT by OPFRs might be involved in the subsequent toxic effects, including intracellular reactive oxygen species (ROS), calcium level, as well as cell proliferation and autophagy.
29559311	10	60	theme	intracellular	1351:1363	arg1	ROS					1390:1392	ROS	1390:1392	ROS	1390:1392	Inhibition of OGT by OPFRs might be involved in the subsequent toxic effects, including intracellular reactive oxygen species (ROS), calcium level, as well as cell proliferation and autophagy.
29559311	0	61	theme	molecular	82:90	arg1	mechanism					92:100	A molecular mechanism	80:100	Inhibition of O-linked N-acetylglucosamine transferase activity in PC12 cells - A molecular mechanism of organophosphate flame retardants developmental neurotoxicity.	0:165	Inhibition of O-linked N-acetylglucosamine transferase activity in PC12 cells - A molecular mechanism of organophosphate flame retardants developmental neurotoxicity.
29559311	12	62	theme	biological	1619:1628	arg1	target					1630:1635	a new biological target	1613:1635	a new biological target	1613:1635	Our findings may provide a new biological target of OPFRs in their neurotoxicological actions, which might be a major molecular mechanism of OPFRs developmental neurotoxicity.
29559311	12	62	theme	biological	1619:1628	arg1	mechanism					1716:1724	a major molecular mechanism	1698:1724	a major molecular mechanism of OPFRs developmental neurotoxicity	1698:1761	Our findings may provide a new biological target of OPFRs in their neurotoxicological actions, which might be a major molecular mechanism of OPFRs developmental neurotoxicity.
29559311	3	63	theme	N-acetylglucosamine	416:434	arg1	OGT					460:462	OGT	460:462	OGT	460:462	O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) regulates numerous neural processes through the O-GlcNAcylation modification of nuclear and cytoplasmic proteins.
29559311	3	63	theme	N-acetylglucosamine	416:434	arg1	transferase					447:457	O-linked N-acetylglucosamine (O-GlcNAc) transferase	407:457	O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT)	407:463	O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) regulates numerous neural processes through the O-GlcNAcylation modification of nuclear and cytoplasmic proteins.
29559311	9	64	theme	0-100 μM	1157:1164	arg1	OPFRs					1150:1154	The six OPFRs	1142:1154	The six OPFRs (0-100 μM exposure)	1142:1174	The six OPFRs (0-100 μM exposure) also inhibited OGT activity in PC12 cells and decreased protein O-GlcNAcylation level.
29559311	9	64	theme	0-100 μM	1157:1164	arg1	exposure					1166:1173	0-100 μM exposure	1157:1173	0-100 μM exposure	1157:1173	The six OPFRs (0-100 μM exposure) also inhibited OGT activity in PC12 cells and decreased protein O-GlcNAcylation level.
29559311	5	65	theme	activity	818:825	arg1	inhibition					800:809	inhibition	800:809	inhibition of OGT activity	800:825	Twelve OPFRs were evaluated for inhibition of OGT activity using an electrochemical biosensor.
29559311	12	66	theme	neurotoxicity	1749:1761	arg1	target					1630:1635	a new biological target	1613:1635	a new biological target	1613:1635	Our findings may provide a new biological target of OPFRs in their neurotoxicological actions, which might be a major molecular mechanism of OPFRs developmental neurotoxicity.
29559311	12	66	theme	neurotoxicity	1749:1761	arg1	mechanism					1716:1724	a major molecular mechanism	1698:1724	a major molecular mechanism of OPFRs developmental neurotoxicity	1698:1761	Our findings may provide a new biological target of OPFRs in their neurotoxicological actions, which might be a major molecular mechanism of OPFRs developmental neurotoxicity.
29559311	10	67	theme	oxygen	1374:1379	arg1	species					1381:1387	intracellular reactive oxygen species	1351:1387	intracellular reactive oxygen species (ROS)	1351:1393	Inhibition of OGT by OPFRs might be involved in the subsequent toxic effects, including intracellular reactive oxygen species (ROS), calcium level, as well as cell proliferation and autophagy.
29559311	10	67	theme	oxygen	1374:1379	arg1	ROS					1390:1392	ROS	1390:1392	ROS	1390:1392	Inhibition of OGT by OPFRs might be involved in the subsequent toxic effects, including intracellular reactive oxygen species (ROS), calcium level, as well as cell proliferation and autophagy.
29559311	0	68	theme	flame	121:125	arg1	retardants					127:136	organophosphate flame retardants developmental neurotoxicity	105:164	organophosphate flame retardants developmental neurotoxicity	105:164	Inhibition of O-linked N-acetylglucosamine transferase activity in PC12 cells - A molecular mechanism of organophosphate flame retardants developmental neurotoxicity.
29559311	10	69	theme	calcium	1396:1402	arg1	level					1404:1408	calcium level	1396:1408	calcium level	1396:1408	Inhibition of OGT by OPFRs might be involved in the subsequent toxic effects, including intracellular reactive oxygen species (ROS), calcium level, as well as cell proliferation and autophagy.
29559311	3	70	theme	neural	484:489	arg1	processes					491:499	numerous neural processes	475:499	numerous neural processes	475:499	O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) regulates numerous neural processes through the O-GlcNAcylation modification of nuclear and cytoplasmic proteins.
29559311	0	71	theme	N-acetylglucosamine	23:41	arg1	transferase					43:53	O-linked N-acetylglucosamine transferase	14:53	O-linked N-acetylglucosamine transferase activity	14:62	Inhibition of O-linked N-acetylglucosamine transferase activity in PC12 cells - A molecular mechanism of organophosphate flame retardants developmental neurotoxicity.
29559311	7	72	dep	group	921:925	arg1	OPFRs					939:943	substituted OPFRs	927:943	The alkyl group substituted OPFRs	911:943	The alkyl group substituted OPFRs had no inhibitory effect.
29559311	0	73	theme	activity	55:62	arg1	Inhibition					0:9	Inhibition	0:9	Inhibition of O-linked N-acetylglucosamine transferase activity in PC12 cells - A molecular mechanism of organophosphate flame retardants developmental neurotoxicity.	0:165	Inhibition of O-linked N-acetylglucosamine transferase activity in PC12 cells - A molecular mechanism of organophosphate flame retardants developmental neurotoxicity.
29559311	11	74	from	difference	1528:1537	arg1	potency					1579:1585	their structure-dependent inhibition potency	1542:1585	their structure-dependent inhibition potency	1542:1585	Molecular docking of the OGT/OPFR complexes provided rationales for the difference in their structure-dependent inhibition potency.
29559311	12	75	theme	OPFRs	1729:1733	arg1	neurotoxicity					1749:1761	OPFRs developmental neurotoxicity	1729:1761	OPFRs developmental neurotoxicity	1729:1761	Our findings may provide a new biological target of OPFRs in their neurotoxicological actions, which might be a major molecular mechanism of OPFRs developmental neurotoxicity.
29559311	9	76	theme	PC12	1207:1210	arg1	cells					1212:1216	PC12 cells	1207:1216	PC12 cells	1207:1216	The six OPFRs (0-100 μM exposure) also inhibited OGT activity in PC12 cells and decreased protein O-GlcNAcylation level.
29559311	3	77	theme	O-GlcNAcylation	513:527	arg1	modification					529:540	the O-GlcNAcylation modification	509:540	the O-GlcNAcylation modification of nuclear and cytoplasmic proteins	509:576	O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) regulates numerous neural processes through the O-GlcNAcylation modification of nuclear and cytoplasmic proteins.
29559311	0	78	theme	PC12	67:70	arg1	cells					72:76	PC12 cells	67:76	PC12 cells	67:76	Inhibition of O-linked N-acetylglucosamine transferase activity in PC12 cells - A molecular mechanism of organophosphate flame retardants developmental neurotoxicity.
29559311	8	79	theme	aromatic	1011:1018	arg1	groups					1041:1046	aromatic or chlorinated alkyl groups	1011:1046	aromatic or chlorinated alkyl groups	1011:1046	Instead, the six OPFRs substituted with aromatic or chlorinated alkyl groups inhibited OGT activity significantly, with tri-m-cresyl phosphate (TCrP) being the strongest.
29559311	7	80	contain	had	945:947	arg2	effect					963:968	no inhibitory effect	949:968	no inhibitory effect	949:968	The alkyl group substituted OPFRs had no inhibitory effect.
29559311	7	80	contain	had	945:947	arg1	group					921:925	The alkyl group substituted OPFRs	911:943	The alkyl group substituted OPFRs	911:943	The alkyl group substituted OPFRs had no inhibitory effect.
29559311	3	81	theme	proteins	569:576	arg1	modification					529:540	the O-GlcNAcylation modification	509:540	the O-GlcNAcylation modification of nuclear and cytoplasmic proteins	509:576	O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) regulates numerous neural processes through the O-GlcNAcylation modification of nuclear and cytoplasmic proteins.
29559311	8	82	theme	chlorinated	1023:1033	arg1	groups					1041:1046	aromatic or chlorinated alkyl groups	1011:1046	aromatic or chlorinated alkyl groups	1011:1046	Instead, the six OPFRs substituted with aromatic or chlorinated alkyl groups inhibited OGT activity significantly, with tri-m-cresyl phosphate (TCrP) being the strongest.
29559311	9	83	theme	protein	1232:1238	arg1	level					1256:1260	protein O-GlcNAcylation level	1232:1260	protein O-GlcNAcylation level	1232:1260	The six OPFRs (0-100 μM exposure) also inhibited OGT activity in PC12 cells and decreased protein O-GlcNAcylation level.
29559311	10	84	theme	OGT	1277:1279	arg1	Inhibition					1263:1272	Inhibition	1263:1272	Inhibition of OGT by OPFRs	1263:1288	Inhibition of OGT by OPFRs might be involved in the subsequent toxic effects, including intracellular reactive oxygen species (ROS), calcium level, as well as cell proliferation and autophagy.
29559311	10	85	theme	cell	1422:1425	arg1	proliferation					1427:1439	cell proliferation	1422:1439	cell proliferation	1422:1439	Inhibition of OGT by OPFRs might be involved in the subsequent toxic effects, including intracellular reactive oxygen species (ROS), calcium level, as well as cell proliferation and autophagy.
30939976	2	0	dep	L.	539:540	arg1	fermentum					542:550	fermentum	542:550	fermentum	542:550	The levels of inflammatory cytokines, such as interleukin (IL)-2, interferon-γ, IL-4, IL-13, and IL-17A, were decreased, and that of the anti-inflammatory cytokine IL-10 was increased, in human peripheral blood mononuclear cells (PBMCs) treated with the L. fermentum KBL374 or KBL375 strain.
30939976	2	1	theme	KBL375	562:567	arg1	strain					569:574	KBL375 strain	562:574	KBL375 strain	562:574	The levels of inflammatory cytokines, such as interleukin (IL)-2, interferon-γ, IL-4, IL-13, and IL-17A, were decreased, and that of the anti-inflammatory cytokine IL-10 was increased, in human peripheral blood mononuclear cells (PBMCs) treated with the L. fermentum KBL374 or KBL375 strain.
30939976	4	2	theme	leukocyte	974:982	arg1	infiltration					984:995	leukocyte infiltration	974:995	leukocyte infiltration	974:995	Furthermore, both L. fermentum KBL374 and KBL375 modulated the innate immune response by improving gut barrier function and reducing leukocyte infiltration.
30939976	1	3	theme	Koreans	276:282	arg1	feces					259:263	feces	259:263	feces of healthy Koreans	259:282	We evaluated immunometabolic functions of novel Lactobacillus fermentum strains (KBL374 and KBL375) isolated from feces of healthy Koreans.
30939976	7	4	theme	fermentum	1370:1378	arg1	KBL374					1380:1385	L. fermentum KBL374 or KBL375 administration	1367:1410	KBL374	1380:1385	Additionally, L. fermentum KBL374 or KBL375 administration reshaped and increased the diversity of the gut microbiota.
30939976	2	5	theme	inflammatory	299:310	arg1	interferon-γ					351:362	interferon-γ	351:362	interferon-γ	351:362	The levels of inflammatory cytokines, such as interleukin (IL)-2, interferon-γ, IL-4, IL-13, and IL-17A, were decreased, and that of the anti-inflammatory cytokine IL-10 was increased, in human peripheral blood mononuclear cells (PBMCs) treated with the L. fermentum KBL374 or KBL375 strain.
30939976	2	5	theme	inflammatory	299:310	arg1	IL-17A					382:387	IL-17A	382:387	IL-17A	382:387	The levels of inflammatory cytokines, such as interleukin (IL)-2, interferon-γ, IL-4, IL-13, and IL-17A, were decreased, and that of the anti-inflammatory cytokine IL-10 was increased, in human peripheral blood mononuclear cells (PBMCs) treated with the L. fermentum KBL374 or KBL375 strain.
30939976	2	5	theme	inflammatory	299:310	arg1	IL-4					365:368	IL-4	365:368	IL-4	365:368	The levels of inflammatory cytokines, such as interleukin (IL)-2, interferon-γ, IL-4, IL-13, and IL-17A, were decreased, and that of the anti-inflammatory cytokine IL-10 was increased, in human peripheral blood mononuclear cells (PBMCs) treated with the L. fermentum KBL374 or KBL375 strain.
30939976	2	5	theme	inflammatory	299:310	arg1	cytokines					312:320	inflammatory cytokines	299:320	inflammatory cytokines	299:320	The levels of inflammatory cytokines, such as interleukin (IL)-2, interferon-γ, IL-4, IL-13, and IL-17A, were decreased, and that of the anti-inflammatory cytokine IL-10 was increased, in human peripheral blood mononuclear cells (PBMCs) treated with the L. fermentum KBL374 or KBL375 strain.
30939976	2	5	theme	inflammatory	299:310	arg1	IL					344:345	interleukin (IL)-2	331:348	interleukin (IL)-2	331:348	The levels of inflammatory cytokines, such as interleukin (IL)-2, interferon-γ, IL-4, IL-13, and IL-17A, were decreased, and that of the anti-inflammatory cytokine IL-10 was increased, in human peripheral blood mononuclear cells (PBMCs) treated with the L. fermentum KBL374 or KBL375 strain.
30939976	2	5	theme	inflammatory	299:310	arg1	IL-13					371:375	IL-13	371:375	IL-13	371:375	The levels of inflammatory cytokines, such as interleukin (IL)-2, interferon-γ, IL-4, IL-13, and IL-17A, were decreased, and that of the anti-inflammatory cytokine IL-10 was increased, in human peripheral blood mononuclear cells (PBMCs) treated with the L. fermentum KBL374 or KBL375 strain.
30939976	5	6	theme	Th17-related	1122:1133	arg1	cytokine					1135:1142	Th17-related cytokine	1122:1142	Th17-related cytokine	1122:1142	Consistent with the PBMC data, both L. fermentum KBL374- and KBL375-treated DSS mice demonstrated decreased Th1-, Th2-, and Th17-related cytokine levels and increased IL-10 in the colon compared with the DSS control mice.
30939976	10	7	theme	inflammatory	1690:1701	arg1	disease					1709:1715	inflammatory bowel disease	1690:1715	inflammatory bowel disease	1690:1715	Both L. fermentum KBL374 and KBL375 may alleviate inflammatory diseases, such as inflammatory bowel disease, in the gut by regulating immune responses and altering the composition of gut microbiota.
30939976	1	8	theme	fermentum	207:215	arg1	strains					217:223	novel Lactobacillus fermentum strains	187:223	novel Lactobacillus fermentum strains (KBL374 and KBL375) isolated from feces of healthy Koreans	187:282	We evaluated immunometabolic functions of novel Lactobacillus fermentum strains (KBL374 and KBL375) isolated from feces of healthy Koreans.
30939976	1	8	theme	fermentum	207:215	arg1	KBL375					237:242	KBL375	237:242	KBL375	237:242	We evaluated immunometabolic functions of novel Lactobacillus fermentum strains (KBL374 and KBL375) isolated from feces of healthy Koreans.
30939976	1	8	theme	fermentum	207:215	arg1	KBL374					226:231	KBL374	226:231	KBL374	226:231	We evaluated immunometabolic functions of novel Lactobacillus fermentum strains (KBL374 and KBL375) isolated from feces of healthy Koreans.
30939976	7	9	theme	KBL375	1390:1395	arg1	administration					1397:1410	L. fermentum KBL374 or KBL375 administration	1367:1410	administration	1397:1410	Additionally, L. fermentum KBL374 or KBL375 administration reshaped and increased the diversity of the gut microbiota.
30939976	6	10	theme	KBL375	1261:1266	arg1	Administration					1220:1233	Administration	1220:1233	Administration of L. fermentum KBL374 or KBL375 to mice	1220:1274	Administration of L. fermentum KBL374 or KBL375 to mice increased the CD4+CD25+Foxp3+Treg cell population in mesenteric lymph nodes.
30939976	4	11	theme	L.	859:860	arg1	KBL374					872:877	L. fermentum KBL374	859:877	L. fermentum KBL374	859:877	Furthermore, both L. fermentum KBL374 and KBL375 modulated the innate immune response by improving gut barrier function and reducing leukocyte infiltration.
30939976	5	12	theme	cytokine	1135:1142	arg1	levels					1144:1149	decreased Th1-, Th2-, and Th17-related cytokine levels	1096:1149	decreased Th1-, Th2-, and Th17-related cytokine levels	1096:1149	Consistent with the PBMC data, both L. fermentum KBL374- and KBL375-treated DSS mice demonstrated decreased Th1-, Th2-, and Th17-related cytokine levels and increased IL-10 in the colon compared with the DSS control mice.
30939976	5	13	theme	PBMC	1018:1021	arg1	data					1023:1026	the PBMC data	1014:1026	the PBMC data	1014:1026	Consistent with the PBMC data, both L. fermentum KBL374- and KBL375-treated DSS mice demonstrated decreased Th1-, Th2-, and Th17-related cytokine levels and increased IL-10 in the colon compared with the DSS control mice.
30939976	1	14	attach	isolated	245:252	arg1	feces					259:263	feces	259:263	feces of healthy Koreans	259:282	We evaluated immunometabolic functions of novel Lactobacillus fermentum strains (KBL374 and KBL375) isolated from feces of healthy Koreans.
30939976	1	14	attach	isolated	245:252	arg2	KBL375					237:242	KBL375	237:242	KBL375	237:242	We evaluated immunometabolic functions of novel Lactobacillus fermentum strains (KBL374 and KBL375) isolated from feces of healthy Koreans.
30939976	1	14	attach	isolated	245:252	arg2	KBL374					226:231	KBL374	226:231	KBL374	226:231	We evaluated immunometabolic functions of novel Lactobacillus fermentum strains (KBL374 and KBL375) isolated from feces of healthy Koreans.
30939976	1	14	attach	isolated	245:252	arg2	strains					217:223	novel Lactobacillus fermentum strains	187:223	novel Lactobacillus fermentum strains (KBL374 and KBL375) isolated from feces of healthy Koreans	187:282	We evaluated immunometabolic functions of novel Lactobacillus fermentum strains (KBL374 and KBL375) isolated from feces of healthy Koreans.
30939976	2	15	theme	L.	539:540	arg1	KBL374					552:557	the L. fermentum KBL374	535:557	the L. fermentum KBL374	535:557	The levels of inflammatory cytokines, such as interleukin (IL)-2, interferon-γ, IL-4, IL-13, and IL-17A, were decreased, and that of the anti-inflammatory cytokine IL-10 was increased, in human peripheral blood mononuclear cells (PBMCs) treated with the L. fermentum KBL374 or KBL375 strain.
30939976	6	16	theme	L.	1238:1239	arg1	KBL374					1251:1256	KBL374	1251:1256	KBL374	1251:1256	Administration of L. fermentum KBL374 or KBL375 to mice increased the CD4+CD25+Foxp3+Treg cell population in mesenteric lymph nodes.
30939976	3	17	theme	colon	788:792	arg1	length					794:799	colon length	788:799	colon length	788:799	When these strains were orally administered to mice with dextran sulfate sodium (DSS)-induced colitis, both L. fermentum KBL374 and KBL375 showed beneficial effects on body weight, disease activity index score, colon length, cecal weight, and histological scores.
30939976	5	18	theme	control	1206:1212	arg1	mice					1214:1217	the DSS control mice	1198:1217	the DSS control mice	1198:1217	Consistent with the PBMC data, both L. fermentum KBL374- and KBL375-treated DSS mice demonstrated decreased Th1-, Th2-, and Th17-related cytokine levels and increased IL-10 in the colon compared with the DSS control mice.
30939976	3	19	theme	body	745:748	arg1	weight					750:755	body weight	745:755	body weight	745:755	When these strains were orally administered to mice with dextran sulfate sodium (DSS)-induced colitis, both L. fermentum KBL374 and KBL375 showed beneficial effects on body weight, disease activity index score, colon length, cecal weight, and histological scores.
30939976	5	20	from	IL-10	1165:1169	arg1	colon					1178:1182	the colon	1174:1182	the colon	1174:1182	Consistent with the PBMC data, both L. fermentum KBL374- and KBL375-treated DSS mice demonstrated decreased Th1-, Th2-, and Th17-related cytokine levels and increased IL-10 in the colon compared with the DSS control mice.
30939976	6	21	from	population	1315:1324	arg1	nodes					1346:1350	mesenteric lymph nodes	1329:1350	mesenteric lymph nodes	1329:1350	Administration of L. fermentum KBL374 or KBL375 to mice increased the CD4+CD25+Foxp3+Treg cell population in mesenteric lymph nodes.
30939976	4	22	theme	immune	911:916	arg1	response					918:925	the innate immune response	900:925	the innate immune response	900:925	Furthermore, both L. fermentum KBL374 and KBL375 modulated the innate immune response by improving gut barrier function and reducing leukocyte infiltration.
30939976	3	23	theme	activity	766:773	arg1	score					781:785	disease activity index score	758:785	disease activity index score	758:785	When these strains were orally administered to mice with dextran sulfate sodium (DSS)-induced colitis, both L. fermentum KBL374 and KBL375 showed beneficial effects on body weight, disease activity index score, colon length, cecal weight, and histological scores.
30939976	6	24	theme	cell	1310:1313	arg1	population					1315:1324	the CD4+CD25+Foxp3+Treg cell population	1286:1324	the CD4+CD25+Foxp3+Treg cell population in mesenteric lymph nodes	1286:1350	Administration of L. fermentum KBL374 or KBL375 to mice increased the CD4+CD25+Foxp3+Treg cell population in mesenteric lymph nodes.
30939976	5	25	theme	KBL375-treated	1059:1072	arg1	mice					1078:1081	both L. fermentum KBL374- and KBL375-treated DSS mice	1029:1081	mice	1078:1081	Consistent with the PBMC data, both L. fermentum KBL374- and KBL375-treated DSS mice demonstrated decreased Th1-, Th2-, and Th17-related cytokine levels and increased IL-10 in the colon compared with the DSS control mice.
30939976	1	26	theme	novel	187:191	arg1	strains					217:223	novel Lactobacillus fermentum strains	187:223	novel Lactobacillus fermentum strains (KBL374 and KBL375) isolated from feces of healthy Koreans	187:282	We evaluated immunometabolic functions of novel Lactobacillus fermentum strains (KBL374 and KBL375) isolated from feces of healthy Koreans.
30939976	1	26	theme	novel	187:191	arg1	KBL375					237:242	KBL375	237:242	KBL375	237:242	We evaluated immunometabolic functions of novel Lactobacillus fermentum strains (KBL374 and KBL375) isolated from feces of healthy Koreans.
30939976	1	26	theme	novel	187:191	arg1	KBL374					226:231	KBL374	226:231	KBL374	226:231	We evaluated immunometabolic functions of novel Lactobacillus fermentum strains (KBL374 and KBL375) isolated from feces of healthy Koreans.
30939976	2	27	theme	mononuclear	496:506	arg1	PBMCs					515:519	PBMCs	515:519	PBMCs	515:519	The levels of inflammatory cytokines, such as interleukin (IL)-2, interferon-γ, IL-4, IL-13, and IL-17A, were decreased, and that of the anti-inflammatory cytokine IL-10 was increased, in human peripheral blood mononuclear cells (PBMCs) treated with the L. fermentum KBL374 or KBL375 strain.
30939976	2	27	theme	mononuclear	496:506	arg1	cells					508:512	human peripheral blood mononuclear cells	473:512	human peripheral blood mononuclear cells (PBMCs) treated with the L. fermentum KBL374 or KBL375 strain	473:574	The levels of inflammatory cytokines, such as interleukin (IL)-2, interferon-γ, IL-4, IL-13, and IL-17A, were decreased, and that of the anti-inflammatory cytokine IL-10 was increased, in human peripheral blood mononuclear cells (PBMCs) treated with the L. fermentum KBL374 or KBL375 strain.
30939976	2	28	theme	peripheral	479:488	arg1	PBMCs					515:519	PBMCs	515:519	PBMCs	515:519	The levels of inflammatory cytokines, such as interleukin (IL)-2, interferon-γ, IL-4, IL-13, and IL-17A, were decreased, and that of the anti-inflammatory cytokine IL-10 was increased, in human peripheral blood mononuclear cells (PBMCs) treated with the L. fermentum KBL374 or KBL375 strain.
30939976	2	28	theme	peripheral	479:488	arg1	cells					508:512	human peripheral blood mononuclear cells	473:512	human peripheral blood mononuclear cells (PBMCs) treated with the L. fermentum KBL374 or KBL375 strain	473:574	The levels of inflammatory cytokines, such as interleukin (IL)-2, interferon-γ, IL-4, IL-13, and IL-17A, were decreased, and that of the anti-inflammatory cytokine IL-10 was increased, in human peripheral blood mononuclear cells (PBMCs) treated with the L. fermentum KBL374 or KBL375 strain.
30939976	5	29	with	Consistent	998:1007	arg1	data					1023:1026	the PBMC data	1014:1026	the PBMC data	1014:1026	Consistent with the PBMC data, both L. fermentum KBL374- and KBL375-treated DSS mice demonstrated decreased Th1-, Th2-, and Th17-related cytokine levels and increased IL-10 in the colon compared with the DSS control mice.
30939976	5	30	theme	L.	1034:1035	arg1	KBL374-					1047:1053	both L. fermentum KBL374- and KBL375-treated DSS mice	1029:1081	KBL374-	1047:1053	Consistent with the PBMC data, both L. fermentum KBL374- and KBL375-treated DSS mice demonstrated decreased Th1-, Th2-, and Th17-related cytokine levels and increased IL-10 in the colon compared with the DSS control mice.
30939976	0	31	theme	Lactobacillus	0:12	arg1	species					24:30	Lactobacillus fermentum species	0:30	Lactobacillus fermentum species	0:30	Lactobacillus fermentum species ameliorate dextran sulfate sodium-induced colitis by regulating the immune response and altering gut microbiota.
30939976	8	32	theme	L.	1487:1488	arg1	KBL375					1500:1505	L. fermentum KBL375	1487:1505	L. fermentum KBL375	1487:1505	In particular, L. fermentum KBL375 increased the abundance of beneficial microorganisms, such as Lactobacillus spp.
30939976	1	33	dep	strains	217:223	arg1	strains					217:223	novel Lactobacillus fermentum strains	187:223	novel Lactobacillus fermentum strains (KBL374 and KBL375) isolated from feces of healthy Koreans	187:282	We evaluated immunometabolic functions of novel Lactobacillus fermentum strains (KBL374 and KBL375) isolated from feces of healthy Koreans.
30939976	1	33	dep	strains	217:223	arg1	KBL375					237:242	KBL375	237:242	KBL375	237:242	We evaluated immunometabolic functions of novel Lactobacillus fermentum strains (KBL374 and KBL375) isolated from feces of healthy Koreans.
30939976	1	33	dep	strains	217:223	arg1	KBL374					226:231	KBL374	226:231	KBL374	226:231	We evaluated immunometabolic functions of novel Lactobacillus fermentum strains (KBL374 and KBL375) isolated from feces of healthy Koreans.
30939976	0	34	theme	dextran	43:49	arg1	sulfate					51:57	dextran sulfate	43:57	dextran sulfate sodium-induced colitis	43:80	Lactobacillus fermentum species ameliorate dextran sulfate sodium-induced colitis by regulating the immune response and altering gut microbiota.
30939976	2	35	theme	anti-inflammatory	422:438	arg1	IL-10					449:453	the anti-inflammatory cytokine IL-10	418:453	the anti-inflammatory cytokine IL-10	418:453	The levels of inflammatory cytokines, such as interleukin (IL)-2, interferon-γ, IL-4, IL-13, and IL-17A, were decreased, and that of the anti-inflammatory cytokine IL-10 was increased, in human peripheral blood mononuclear cells (PBMCs) treated with the L. fermentum KBL374 or KBL375 strain.
30939976	3	36	from	effects	734:740	arg1	scores					833:838	histological scores	820:838	histological scores	820:838	When these strains were orally administered to mice with dextran sulfate sodium (DSS)-induced colitis, both L. fermentum KBL374 and KBL375 showed beneficial effects on body weight, disease activity index score, colon length, cecal weight, and histological scores.
30939976	3	36	from	effects	734:740	arg1	score					781:785	disease activity index score	758:785	disease activity index score	758:785	When these strains were orally administered to mice with dextran sulfate sodium (DSS)-induced colitis, both L. fermentum KBL374 and KBL375 showed beneficial effects on body weight, disease activity index score, colon length, cecal weight, and histological scores.
30939976	3	36	from	effects	734:740	arg1	length					794:799	colon length	788:799	colon length	788:799	When these strains were orally administered to mice with dextran sulfate sodium (DSS)-induced colitis, both L. fermentum KBL374 and KBL375 showed beneficial effects on body weight, disease activity index score, colon length, cecal weight, and histological scores.
30939976	3	36	from	effects	734:740	arg1	weight					808:813	cecal weight	802:813	cecal weight	802:813	When these strains were orally administered to mice with dextran sulfate sodium (DSS)-induced colitis, both L. fermentum KBL374 and KBL375 showed beneficial effects on body weight, disease activity index score, colon length, cecal weight, and histological scores.
30939976	3	36	from	effects	734:740	arg1	weight					750:755	body weight	745:755	body weight	745:755	When these strains were orally administered to mice with dextran sulfate sodium (DSS)-induced colitis, both L. fermentum KBL374 and KBL375 showed beneficial effects on body weight, disease activity index score, colon length, cecal weight, and histological scores.
30939976	10	37	theme	gut	1792:1794	arg1	microbiota					1796:1805	gut microbiota	1792:1805	gut microbiota	1792:1805	Both L. fermentum KBL374 and KBL375 may alleviate inflammatory diseases, such as inflammatory bowel disease, in the gut by regulating immune responses and altering the composition of gut microbiota.
30939976	10	38	theme	inflammatory	1659:1670	arg1	disease					1709:1715	inflammatory bowel disease	1690:1715	inflammatory bowel disease	1690:1715	Both L. fermentum KBL374 and KBL375 may alleviate inflammatory diseases, such as inflammatory bowel disease, in the gut by regulating immune responses and altering the composition of gut microbiota.
30939976	10	38	theme	inflammatory	1659:1670	arg1	diseases					1672:1679	inflammatory diseases	1659:1679	inflammatory diseases	1659:1679	Both L. fermentum KBL374 and KBL375 may alleviate inflammatory diseases, such as inflammatory bowel disease, in the gut by regulating immune responses and altering the composition of gut microbiota.
30939976	6	39	theme	lymph	1340:1344	arg1	nodes					1346:1350	mesenteric lymph nodes	1329:1350	mesenteric lymph nodes	1329:1350	Administration of L. fermentum KBL374 or KBL375 to mice increased the CD4+CD25+Foxp3+Treg cell population in mesenteric lymph nodes.
30939976	5	40	theme	decreased	1096:1104	arg1	levels					1144:1149	decreased Th1-, Th2-, and Th17-related cytokine levels	1096:1149	decreased Th1-, Th2-, and Th17-related cytokine levels	1096:1149	Consistent with the PBMC data, both L. fermentum KBL374- and KBL375-treated DSS mice demonstrated decreased Th1-, Th2-, and Th17-related cytokine levels and increased IL-10 in the colon compared with the DSS control mice.
30939976	7	41	theme	gut	1456:1458	arg1	microbiota					1460:1469	the gut microbiota	1452:1469	the gut microbiota	1452:1469	Additionally, L. fermentum KBL374 or KBL375 administration reshaped and increased the diversity of the gut microbiota.
30939976	8	42	theme	microorganisms	1545:1558	arg1	abundance					1521:1529	the abundance	1517:1529	the abundance of beneficial microorganisms, such as Lactobacillus spp	1517:1585	In particular, L. fermentum KBL375 increased the abundance of beneficial microorganisms, such as Lactobacillus spp.
30939976	4	43	theme	gut	940:942	arg1	function					952:959	gut barrier function	940:959	gut barrier function	940:959	Furthermore, both L. fermentum KBL374 and KBL375 modulated the innate immune response by improving gut barrier function and reducing leukocyte infiltration.
30939976	1	44	theme	healthy	268:274	arg1	Koreans					276:282	healthy Koreans	268:282	healthy Koreans	268:282	We evaluated immunometabolic functions of novel Lactobacillus fermentum strains (KBL374 and KBL375) isolated from feces of healthy Koreans.
30939976	8	45	theme	Lactobacillus	1569:1581	arg1	spp					1583:1585	Lactobacillus spp	1569:1585	Lactobacillus spp	1569:1585	In particular, L. fermentum KBL375 increased the abundance of beneficial microorganisms, such as Lactobacillus spp.
30939976	2	46	theme	cytokines	312:320	arg1	levels					289:294	The levels	285:294	The levels of inflammatory cytokines, such as interleukin (IL)-2, interferon-γ, IL-4, IL-13, and IL-17A,	285:388	The levels of inflammatory cytokines, such as interleukin (IL)-2, interferon-γ, IL-4, IL-13, and IL-17A, were decreased, and that of the anti-inflammatory cytokine IL-10 was increased, in human peripheral blood mononuclear cells (PBMCs) treated with the L. fermentum KBL374 or KBL375 strain.
30939976	10	47	theme	bowel	1703:1707	arg1	disease					1709:1715	inflammatory bowel disease	1690:1715	inflammatory bowel disease	1690:1715	Both L. fermentum KBL374 and KBL375 may alleviate inflammatory diseases, such as inflammatory bowel disease, in the gut by regulating immune responses and altering the composition of gut microbiota.
30939976	3	48	with	mice	624:627	arg1	colitis					671:677	dextran sulfate sodium (DSS)-induced colitis	634:677	dextran sulfate sodium (DSS)-induced colitis	634:677	When these strains were orally administered to mice with dextran sulfate sodium (DSS)-induced colitis, both L. fermentum KBL374 and KBL375 showed beneficial effects on body weight, disease activity index score, colon length, cecal weight, and histological scores.
30939976	1	49	theme	Lactobacillus	193:205	arg1	strains					217:223	novel Lactobacillus fermentum strains	187:223	novel Lactobacillus fermentum strains (KBL374 and KBL375) isolated from feces of healthy Koreans	187:282	We evaluated immunometabolic functions of novel Lactobacillus fermentum strains (KBL374 and KBL375) isolated from feces of healthy Koreans.
30939976	1	49	theme	Lactobacillus	193:205	arg1	KBL375					237:242	KBL375	237:242	KBL375	237:242	We evaluated immunometabolic functions of novel Lactobacillus fermentum strains (KBL374 and KBL375) isolated from feces of healthy Koreans.
30939976	1	49	theme	Lactobacillus	193:205	arg1	KBL374					226:231	KBL374	226:231	KBL374	226:231	We evaluated immunometabolic functions of novel Lactobacillus fermentum strains (KBL374 and KBL375) isolated from feces of healthy Koreans.
30939976	0	50	theme	sodium-induced	59:72	arg1	colitis					74:80	dextran sulfate sodium-induced colitis	43:80	dextran sulfate sodium-induced colitis	43:80	Lactobacillus fermentum species ameliorate dextran sulfate sodium-induced colitis by regulating the immune response and altering gut microbiota.
30939976	7	51	theme	L.	1367:1368	arg1	KBL374					1380:1385	L. fermentum KBL374 or KBL375 administration	1367:1410	KBL374	1380:1385	Additionally, L. fermentum KBL374 or KBL375 administration reshaped and increased the diversity of the gut microbiota.
30939976	3	52	theme	-induced	662:669	arg1	colitis					671:677	dextran sulfate sodium (DSS)-induced colitis	634:677	dextran sulfate sodium (DSS)-induced colitis	634:677	When these strains were orally administered to mice with dextran sulfate sodium (DSS)-induced colitis, both L. fermentum KBL374 and KBL375 showed beneficial effects on body weight, disease activity index score, colon length, cecal weight, and histological scores.
30939976	2	53	theme	interleukin	331:341	arg1	IL					344:345	interleukin (IL)-2	331:348	interleukin (IL)-2	331:348	The levels of inflammatory cytokines, such as interleukin (IL)-2, interferon-γ, IL-4, IL-13, and IL-17A, were decreased, and that of the anti-inflammatory cytokine IL-10 was increased, in human peripheral blood mononuclear cells (PBMCs) treated with the L. fermentum KBL374 or KBL375 strain.
30939976	1	54	theme	strains	217:223	arg1	functions					174:182	immunometabolic functions	158:182	immunometabolic functions of novel Lactobacillus fermentum strains (KBL374 and KBL375) isolated from feces of healthy Koreans	158:282	We evaluated immunometabolic functions of novel Lactobacillus fermentum strains (KBL374 and KBL375) isolated from feces of healthy Koreans.
30939976	3	55	theme	L.	685:686	arg1	KBL374					698:703	L. fermentum KBL374	685:703	L. fermentum KBL374	685:703	When these strains were orally administered to mice with dextran sulfate sodium (DSS)-induced colitis, both L. fermentum KBL374 and KBL375 showed beneficial effects on body weight, disease activity index score, colon length, cecal weight, and histological scores.
30939976	6	56	dep	KBL374	1251:1256	arg1	fermentum					1241:1249	L. fermentum KBL374 or KBL375	1238:1266	L. fermentum KBL374 or KBL375	1238:1266	Administration of L. fermentum KBL374 or KBL375 to mice increased the CD4+CD25+Foxp3+Treg cell population in mesenteric lymph nodes.
30939976	4	57	dep	L.	859:860	arg1	fermentum					862:870	fermentum	862:870	fermentum	862:870	Furthermore, both L. fermentum KBL374 and KBL375 modulated the innate immune response by improving gut barrier function and reducing leukocyte infiltration.
30939976	10	58	theme	immune	1743:1748	arg1	responses					1750:1758	immune responses	1743:1758	immune responses	1743:1758	Both L. fermentum KBL374 and KBL375 may alleviate inflammatory diseases, such as inflammatory bowel disease, in the gut by regulating immune responses and altering the composition of gut microbiota.
30939976	5	59	theme	DSS	1202:1204	arg1	mice					1214:1217	the DSS control mice	1198:1217	the DSS control mice	1198:1217	Consistent with the PBMC data, both L. fermentum KBL374- and KBL375-treated DSS mice demonstrated decreased Th1-, Th2-, and Th17-related cytokine levels and increased IL-10 in the colon compared with the DSS control mice.
30939976	6	60	theme	mesenteric	1329:1338	arg1	nodes					1346:1350	mesenteric lymph nodes	1329:1350	mesenteric lymph nodes	1329:1350	Administration of L. fermentum KBL374 or KBL375 to mice increased the CD4+CD25+Foxp3+Treg cell population in mesenteric lymph nodes.
30939976	1	61	theme	immunometabolic	158:172	arg1	functions					174:182	immunometabolic functions	158:182	immunometabolic functions of novel Lactobacillus fermentum strains (KBL374 and KBL375) isolated from feces of healthy Koreans	158:282	We evaluated immunometabolic functions of novel Lactobacillus fermentum strains (KBL374 and KBL375) isolated from feces of healthy Koreans.
30939976	0	62	theme	immune	100:105	arg1	response					107:114	the immune response	96:114	the immune response	96:114	Lactobacillus fermentum species ameliorate dextran sulfate sodium-induced colitis by regulating the immune response and altering gut microbiota.
30939976	3	63	dep	L.	685:686	arg1	fermentum					688:696	fermentum	688:696	fermentum	688:696	When these strains were orally administered to mice with dextran sulfate sodium (DSS)-induced colitis, both L. fermentum KBL374 and KBL375 showed beneficial effects on body weight, disease activity index score, colon length, cecal weight, and histological scores.
30939976	4	64	theme	innate	904:909	arg1	response					918:925	the innate immune response	900:925	the innate immune response	900:925	Furthermore, both L. fermentum KBL374 and KBL375 modulated the innate immune response by improving gut barrier function and reducing leukocyte infiltration.
30939976	3	65	theme	index	775:779	arg1	score					781:785	disease activity index score	758:785	disease activity index score	758:785	When these strains were orally administered to mice with dextran sulfate sodium (DSS)-induced colitis, both L. fermentum KBL374 and KBL375 showed beneficial effects on body weight, disease activity index score, colon length, cecal weight, and histological scores.
30939976	6	66	theme	CD4+CD25+Foxp3+Treg	1290:1308	arg1	population					1315:1324	the CD4+CD25+Foxp3+Treg cell population	1286:1324	the CD4+CD25+Foxp3+Treg cell population in mesenteric lymph nodes	1286:1350	Administration of L. fermentum KBL374 or KBL375 to mice increased the CD4+CD25+Foxp3+Treg cell population in mesenteric lymph nodes.
30939976	5	67	theme	DSS	1074:1076	arg1	mice					1078:1081	both L. fermentum KBL374- and KBL375-treated DSS mice	1029:1081	mice	1078:1081	Consistent with the PBMC data, both L. fermentum KBL374- and KBL375-treated DSS mice demonstrated decreased Th1-, Th2-, and Th17-related cytokine levels and increased IL-10 in the colon compared with the DSS control mice.
30939976	3	68	theme	cecal	802:806	arg1	weight					808:813	cecal weight	802:813	cecal weight	802:813	When these strains were orally administered to mice with dextran sulfate sodium (DSS)-induced colitis, both L. fermentum KBL374 and KBL375 showed beneficial effects on body weight, disease activity index score, colon length, cecal weight, and histological scores.
30939976	2	69	theme	blood	490:494	arg1	PBMCs					515:519	PBMCs	515:519	PBMCs	515:519	The levels of inflammatory cytokines, such as interleukin (IL)-2, interferon-γ, IL-4, IL-13, and IL-17A, were decreased, and that of the anti-inflammatory cytokine IL-10 was increased, in human peripheral blood mononuclear cells (PBMCs) treated with the L. fermentum KBL374 or KBL375 strain.
30939976	2	69	theme	blood	490:494	arg1	cells					508:512	human peripheral blood mononuclear cells	473:512	human peripheral blood mononuclear cells (PBMCs) treated with the L. fermentum KBL374 or KBL375 strain	473:574	The levels of inflammatory cytokines, such as interleukin (IL)-2, interferon-γ, IL-4, IL-13, and IL-17A, were decreased, and that of the anti-inflammatory cytokine IL-10 was increased, in human peripheral blood mononuclear cells (PBMCs) treated with the L. fermentum KBL374 or KBL375 strain.
30939976	3	70	theme	disease	758:764	arg1	score					781:785	disease activity index score	758:785	disease activity index score	758:785	When these strains were orally administered to mice with dextran sulfate sodium (DSS)-induced colitis, both L. fermentum KBL374 and KBL375 showed beneficial effects on body weight, disease activity index score, colon length, cecal weight, and histological scores.
30939976	0	71	theme	gut	129:131	arg1	microbiota					133:142	gut microbiota	129:142	gut microbiota	129:142	Lactobacillus fermentum species ameliorate dextran sulfate sodium-induced colitis by regulating the immune response and altering gut microbiota.
30939976	2	72	theme	human	473:477	arg1	PBMCs					515:519	PBMCs	515:519	PBMCs	515:519	The levels of inflammatory cytokines, such as interleukin (IL)-2, interferon-γ, IL-4, IL-13, and IL-17A, were decreased, and that of the anti-inflammatory cytokine IL-10 was increased, in human peripheral blood mononuclear cells (PBMCs) treated with the L. fermentum KBL374 or KBL375 strain.
30939976	2	72	theme	human	473:477	arg1	cells					508:512	human peripheral blood mononuclear cells	473:512	human peripheral blood mononuclear cells (PBMCs) treated with the L. fermentum KBL374 or KBL375 strain	473:574	The levels of inflammatory cytokines, such as interleukin (IL)-2, interferon-γ, IL-4, IL-13, and IL-17A, were decreased, and that of the anti-inflammatory cytokine IL-10 was increased, in human peripheral blood mononuclear cells (PBMCs) treated with the L. fermentum KBL374 or KBL375 strain.
30939976	0	73	theme	fermentum	14:22	arg1	species					24:30	Lactobacillus fermentum species	0:30	Lactobacillus fermentum species	0:30	Lactobacillus fermentum species ameliorate dextran sulfate sodium-induced colitis by regulating the immune response and altering gut microbiota.
30939976	5	74	theme	fermentum	1037:1045	arg1	KBL374-					1047:1053	both L. fermentum KBL374- and KBL375-treated DSS mice	1029:1081	KBL374-	1047:1053	Consistent with the PBMC data, both L. fermentum KBL374- and KBL375-treated DSS mice demonstrated decreased Th1-, Th2-, and Th17-related cytokine levels and increased IL-10 in the colon compared with the DSS control mice.
30939976	3	75	theme	histological	820:831	arg1	scores					833:838	histological scores	820:838	histological scores	820:838	When these strains were orally administered to mice with dextran sulfate sodium (DSS)-induced colitis, both L. fermentum KBL374 and KBL375 showed beneficial effects on body weight, disease activity index score, colon length, cecal weight, and histological scores.
30939976	4	76	theme	barrier	944:950	arg1	function					952:959	gut barrier function	940:959	gut barrier function	940:959	Furthermore, both L. fermentum KBL374 and KBL375 modulated the innate immune response by improving gut barrier function and reducing leukocyte infiltration.
30939976	8	77	theme	fermentum	1490:1498	arg1	KBL375					1500:1505	L. fermentum KBL375	1487:1505	L. fermentum KBL375	1487:1505	In particular, L. fermentum KBL375 increased the abundance of beneficial microorganisms, such as Lactobacillus spp.
30939976	5	78	theme	Th2-	1112:1115	arg1	levels					1144:1149	decreased Th1-, Th2-, and Th17-related cytokine levels	1096:1149	decreased Th1-, Th2-, and Th17-related cytokine levels	1096:1149	Consistent with the PBMC data, both L. fermentum KBL374- and KBL375-treated DSS mice demonstrated decreased Th1-, Th2-, and Th17-related cytokine levels and increased IL-10 in the colon compared with the DSS control mice.
30939976	0	79	theme	sulfate	51:57	arg1	colitis					74:80	dextran sulfate sodium-induced colitis	43:80	dextran sulfate sodium-induced colitis	43:80	Lactobacillus fermentum species ameliorate dextran sulfate sodium-induced colitis by regulating the immune response and altering gut microbiota.
30939976	2	80	theme	cytokine	440:447	arg1	IL-10					449:453	the anti-inflammatory cytokine IL-10	418:453	the anti-inflammatory cytokine IL-10	418:453	The levels of inflammatory cytokines, such as interleukin (IL)-2, interferon-γ, IL-4, IL-13, and IL-17A, were decreased, and that of the anti-inflammatory cytokine IL-10 was increased, in human peripheral blood mononuclear cells (PBMCs) treated with the L. fermentum KBL374 or KBL375 strain.
30939976	10	81	theme	microbiota	1796:1805	arg1	composition					1777:1787	the composition	1773:1787	the composition of gut microbiota	1773:1805	Both L. fermentum KBL374 and KBL375 may alleviate inflammatory diseases, such as inflammatory bowel disease, in the gut by regulating immune responses and altering the composition of gut microbiota.
30939976	10	82	dep	L.	1614:1615	arg1	fermentum					1617:1625	fermentum	1617:1625	fermentum	1617:1625	Both L. fermentum KBL374 and KBL375 may alleviate inflammatory diseases, such as inflammatory bowel disease, in the gut by regulating immune responses and altering the composition of gut microbiota.
30939976	10	83	theme	L.	1614:1615	arg1	KBL374					1627:1632	L. fermentum KBL374	1614:1632	L. fermentum KBL374	1614:1632	Both L. fermentum KBL374 and KBL375 may alleviate inflammatory diseases, such as inflammatory bowel disease, in the gut by regulating immune responses and altering the composition of gut microbiota.
30939976	6	84	theme	KBL374	1251:1256	arg1	Administration					1220:1233	Administration	1220:1233	Administration of L. fermentum KBL374 or KBL375 to mice	1220:1274	Administration of L. fermentum KBL374 or KBL375 to mice increased the CD4+CD25+Foxp3+Treg cell population in mesenteric lymph nodes.
30939976	8	85	theme	beneficial	1534:1543	arg1	spp					1583:1585	Lactobacillus spp	1569:1585	Lactobacillus spp	1569:1585	In particular, L. fermentum KBL375 increased the abundance of beneficial microorganisms, such as Lactobacillus spp.
30939976	8	85	theme	beneficial	1534:1543	arg1	microorganisms					1545:1558	beneficial microorganisms	1534:1558	beneficial microorganisms	1534:1558	In particular, L. fermentum KBL375 increased the abundance of beneficial microorganisms, such as Lactobacillus spp.
30939976	5	86	theme	Th1-	1106:1109	arg1	levels					1144:1149	decreased Th1-, Th2-, and Th17-related cytokine levels	1096:1149	decreased Th1-, Th2-, and Th17-related cytokine levels	1096:1149	Consistent with the PBMC data, both L. fermentum KBL374- and KBL375-treated DSS mice demonstrated decreased Th1-, Th2-, and Th17-related cytokine levels and increased IL-10 in the colon compared with the DSS control mice.
30939976	7	87	theme	microbiota	1460:1469	arg1	diversity					1439:1447	the diversity	1435:1447	the diversity of the gut microbiota	1435:1469	Additionally, L. fermentum KBL374 or KBL375 administration reshaped and increased the diversity of the gut microbiota.
30939976	3	88	theme	beneficial	723:732	arg1	effects					734:740	beneficial effects	723:740	beneficial effects on body weight, disease activity index score, colon length, cecal weight, and histological scores	723:838	When these strains were orally administered to mice with dextran sulfate sodium (DSS)-induced colitis, both L. fermentum KBL374 and KBL375 showed beneficial effects on body weight, disease activity index score, colon length, cecal weight, and histological scores.
31818905	8	0	theme	different	1079:1087	arg1	composition					1089:1099	different composition	1079:1099	different composition	1079:1099	The sieve plate plugging structures of host plants were shown to have different composition in different citrus tissues.
31818905	6	1	theme	cells	859:863	arg1	membrane					836:843	the plasma membrane	825:843	the plasma membrane of the phloem cells	825:863	CLas cells adhere to the plasma membrane of the phloem cells specifically adjacent to the sieve pores.
31818905	4	2	theme	orange	593:598	arg1	pores					578:582	phloem pores	571:582	phloem pores of sweet orange (Citrus sinensis) and grapefruit (Citrus paradisi)	571:649	Here, we show that CLas movement through phloem pores of sweet orange (Citrus sinensis) and grapefruit (Citrus paradisi) is carried out by the elongated form of the bacteria.
31818905	2	3	theme	protein	372:378	arg1	induction					380:388	callose and phloem protein induction	353:388	callose and phloem protein induction	353:388	HLB-infected citrus phloem cells undergo structural modifications that include cell wall thickening, callose and phloem protein induction, and cellular plugging.
31818905	4	4	theme	Citrus	601:606	arg1	orange					593:598	sweet orange	587:598	sweet orange (Citrus sinensis)	587:616	Here, we show that CLas movement through phloem pores of sweet orange (Citrus sinensis) and grapefruit (Citrus paradisi) is carried out by the elongated form of the bacteria.
31818905	4	4	theme	Citrus	601:606	arg1	sinensis					608:615	Citrus sinensis	601:615	Citrus sinensis	601:615	Here, we show that CLas movement through phloem pores of sweet orange (Citrus sinensis) and grapefruit (Citrus paradisi) is carried out by the elongated form of the bacteria.
31818905	10	5	theme	extracellular	1298:1310	arg1	material					1312:1319	dark extracellular material	1293:1319	dark extracellular material	1293:1319	In the roots, pores were surrounded by dark extracellular material, with very little accumulation of callose.
31818905	8	6	theme	citrus	1114:1119	arg1	tissues					1121:1127	different citrus tissues	1104:1127	different citrus tissues	1104:1127	The sieve plate plugging structures of host plants were shown to have different composition in different citrus tissues.
31818905	1	7	theme	phloem-limited	157:170	arg1	bacterium					200:208	the phloem-limited intracellular Gram-negative bacterium	153:208	the phloem-limited intracellular Gram-negative bacterium Candidatus Liberibacter asiaticus (CLas)	153:249	Citrus greening or Huanglongbing (HLB) is caused by the phloem-limited intracellular Gram-negative bacterium Candidatus Liberibacter asiaticus (CLas).
31818905	7	8	from	present	928:934	arg1	cells					1002:1006	nucleated nonsieve element cells	975:1006	nucleated nonsieve element cells	975:1006	Remarkably, CLas was present in both mature sieve element cells and nucleated nonsieve element cells.
31818905	7	8	from	present	928:934	arg1	cells					965:969	mature sieve element cells	944:969	mature sieve element cells	944:969	Remarkably, CLas was present in both mature sieve element cells and nucleated nonsieve element cells.
31818905	13	9	theme	movement	1675:1682	arg1	mechanisms					1624:1633	the cellular mechanisms	1611:1633	the cellular mechanisms of Gram-negative bacterial cell-to-cell movement in plant phloem	1611:1698	Our results provide insight into the cellular mechanisms of Gram-negative bacterial cell-to-cell movement in plant phloem.
31818905	9	10	theme	main	1157:1160	arg1	mechanism					1171:1179	the main plugging mechanism	1153:1179	the main plugging mechanism in the HLB-infected flush, where it reduced the open space of the pores	1153:1251	Callose deposition was the main plugging mechanism in the HLB-infected flush, where it reduced the open space of the pores.
31818905	9	10	theme	main	1157:1160	arg1	deposition					1138:1147	Callose deposition	1130:1147	Callose deposition	1130:1147	Callose deposition was the main plugging mechanism in the HLB-infected flush, where it reduced the open space of the pores.
31818905	2	11	theme	wall	336:339	arg1	thickening					341:350	cell wall thickening	331:350	cell wall thickening	331:350	HLB-infected citrus phloem cells undergo structural modifications that include cell wall thickening, callose and phloem protein induction, and cellular plugging.
31818905	1	12	theme	Gram-negative	186:198	arg1	bacterium					200:208	the phloem-limited intracellular Gram-negative bacterium	153:208	the phloem-limited intracellular Gram-negative bacterium Candidatus Liberibacter asiaticus (CLas)	153:249	Citrus greening or Huanglongbing (HLB) is caused by the phloem-limited intracellular Gram-negative bacterium Candidatus Liberibacter asiaticus (CLas).
31818905	4	13	theme	Citrus	634:639	arg1	paradisi					641:648	Citrus paradisi	634:648	Citrus paradisi	634:648	Here, we show that CLas movement through phloem pores of sweet orange (Citrus sinensis) and grapefruit (Citrus paradisi) is carried out by the elongated form of the bacteria.
31818905	4	13	theme	Citrus	634:639	arg1	grapefruit					622:631	grapefruit	622:631	grapefruit (Citrus paradisi)	622:649	Here, we show that CLas movement through phloem pores of sweet orange (Citrus sinensis) and grapefruit (Citrus paradisi) is carried out by the elongated form of the bacteria.
31818905	13	14	theme	plant	1687:1691	arg1	phloem					1693:1698	plant phloem	1687:1698	plant phloem	1687:1698	Our results provide insight into the cellular mechanisms of Gram-negative bacterial cell-to-cell movement in plant phloem.
31818905	7	15	from	cells	1002:1006	arg1	present					928:934	present	928:934	present	928:934	Remarkably, CLas was present in both mature sieve element cells and nucleated nonsieve element cells.
31818905	13	16	from	phloem	1693:1698	arg1	mechanisms					1624:1633	the cellular mechanisms	1611:1633	the cellular mechanisms of Gram-negative bacterial cell-to-cell movement in plant phloem	1611:1698	Our results provide insight into the cellular mechanisms of Gram-negative bacterial cell-to-cell movement in plant phloem.
31818905	7	17	theme	sieve	951:955	arg1	cells					965:969	mature sieve element cells	944:969	mature sieve element cells	944:969	Remarkably, CLas was present in both mature sieve element cells and nucleated nonsieve element cells.
31818905	4	18	theme	bacteria	695:702	arg1	form					683:686	the elongated form	669:686	the elongated form of the bacteria	669:702	Here, we show that CLas movement through phloem pores of sweet orange (Citrus sinensis) and grapefruit (Citrus paradisi) is carried out by the elongated form of the bacteria.
31818905	13	19	theme	bacterial	1652:1660	arg1	movement					1675:1682	Gram-negative bacterial cell-to-cell movement	1638:1682	Gram-negative bacterial cell-to-cell movement in plant phloem	1638:1698	Our results provide insight into the cellular mechanisms of Gram-negative bacterial cell-to-cell movement in plant phloem.
31818905	7	20	theme	nonsieve	985:992	arg1	cells					1002:1006	nucleated nonsieve element cells	975:1006	nucleated nonsieve element cells	975:1006	Remarkably, CLas was present in both mature sieve element cells and nucleated nonsieve element cells.
31818905	11	21	theme	genes	1420:1424	arg1	expression					1368:1377	The expression	1364:1377	The expression of CALLOSE SYNTHASE7 and PHLOEM PROTEIN2 genes	1364:1424	The expression of CALLOSE SYNTHASE7 and PHLOEM PROTEIN2 genes was upregulated in the shoots, but downregulated in root tissues.
31818905	2	22	theme	phloem	272:277	arg1	cells					279:283	HLB-infected citrus phloem cells	252:283	HLB-infected citrus phloem cells	252:283	HLB-infected citrus phloem cells undergo structural modifications that include cell wall thickening, callose and phloem protein induction, and cellular plugging.
31818905	4	23	theme	elongated	673:681	arg1	form					683:686	the elongated form	669:686	the elongated form of the bacteria	669:702	Here, we show that CLas movement through phloem pores of sweet orange (Citrus sinensis) and grapefruit (Citrus paradisi) is carried out by the elongated form of the bacteria.
31818905	11	24	theme	PHLOEM	1404:1409	arg1	genes					1420:1424	PHLOEM PROTEIN2 genes	1404:1424	PHLOEM PROTEIN2 genes	1404:1424	The expression of CALLOSE SYNTHASE7 and PHLOEM PROTEIN2 genes was upregulated in the shoots, but downregulated in root tissues.
31818905	0	25	theme	Plugging	70:77	arg1	Dynamics					0:7	Dynamics	0:7	Dynamics of Candidatus Liberibacter asiaticus Movement and Sieve-Pore Plugging in Citrus Sink Cells.	0:99	Dynamics of Candidatus Liberibacter asiaticus Movement and Sieve-Pore Plugging in Citrus Sink Cells.
31818905	2	26	theme	HLB-infected	252:263	arg1	cells					279:283	HLB-infected citrus phloem cells	252:283	HLB-infected citrus phloem cells	252:283	HLB-infected citrus phloem cells undergo structural modifications that include cell wall thickening, callose and phloem protein induction, and cellular plugging.
31818905	0	27	theme	Citrus	82:87	arg1	Cells					94:98	Citrus Sink Cells	82:98	Citrus Sink Cells	82:98	Dynamics of Candidatus Liberibacter asiaticus Movement and Sieve-Pore Plugging in Citrus Sink Cells.
31818905	9	28	theme	open	1229:1232	arg1	space					1234:1238	the open space	1225:1238	the open space of the pores	1225:1251	Callose deposition was the main plugging mechanism in the HLB-infected flush, where it reduced the open space of the pores.
31818905	0	29	from	Dynamics	0:7	arg1	Cells					94:98	Citrus Sink Cells	82:98	Citrus Sink Cells	82:98	Dynamics of Candidatus Liberibacter asiaticus Movement and Sieve-Pore Plugging in Citrus Sink Cells.
31818905	10	30	theme	callose	1355:1361	arg1	accumulation					1339:1350	very little accumulation	1327:1350	very little accumulation of callose	1327:1361	In the roots, pores were surrounded by dark extracellular material, with very little accumulation of callose.
31818905	9	31	theme	Callose	1130:1136	arg1	mechanism					1171:1179	the main plugging mechanism	1153:1179	the main plugging mechanism in the HLB-infected flush, where it reduced the open space of the pores	1153:1251	Callose deposition was the main plugging mechanism in the HLB-infected flush, where it reduced the open space of the pores.
31818905	9	31	theme	Callose	1130:1136	arg1	deposition					1138:1147	Callose deposition	1130:1147	Callose deposition	1130:1147	Callose deposition was the main plugging mechanism in the HLB-infected flush, where it reduced the open space of the pores.
31818905	5	32	theme	CLas	723:726	arg1	large					735:739	large	735:739	large	735:739	The round form of CLas is too large to move, but can change its morphology to enable its movement.
31818905	5	32	theme	CLas	723:726	arg1	form					715:718	The round form	705:718	The round form of CLas	705:726	The round form of CLas is too large to move, but can change its morphology to enable its movement.
31818905	9	33	theme	pores	1247:1251	arg1	space					1234:1238	the open space	1225:1238	the open space of the pores	1225:1251	Callose deposition was the main plugging mechanism in the HLB-infected flush, where it reduced the open space of the pores.
31818905	9	34	from	mechanism	1171:1179	arg1	flush					1201:1205	the HLB-infected flush	1184:1205	the HLB-infected flush	1184:1205	Callose deposition was the main plugging mechanism in the HLB-infected flush, where it reduced the open space of the pores.
31818905	11	35	theme	SYNTHASE7	1390:1398	arg1	expression					1368:1377	The expression	1364:1377	The expression of CALLOSE SYNTHASE7 and PHLOEM PROTEIN2 genes	1364:1424	The expression of CALLOSE SYNTHASE7 and PHLOEM PROTEIN2 genes was upregulated in the shoots, but downregulated in root tissues.
31818905	0	36	theme	Candidatus	12:21	arg1	Movement					46:53	Candidatus Liberibacter asiaticus Movement	12:53	Candidatus Liberibacter asiaticus Movement	12:53	Dynamics of Candidatus Liberibacter asiaticus Movement and Sieve-Pore Plugging in Citrus Sink Cells.
31818905	8	37	theme	sieve	1013:1017	arg1	structures					1034:1043	The sieve plate plugging structures	1009:1043	The sieve plate plugging structures of host plants	1009:1058	The sieve plate plugging structures of host plants were shown to have different composition in different citrus tissues.
31818905	0	38	theme	asiaticus	36:44	arg1	Movement					46:53	Candidatus Liberibacter asiaticus Movement	12:53	Candidatus Liberibacter asiaticus Movement	12:53	Dynamics of Candidatus Liberibacter asiaticus Movement and Sieve-Pore Plugging in Citrus Sink Cells.
31818905	8	39	theme	plugging	1025:1032	arg1	structures					1034:1043	The sieve plate plugging structures	1009:1043	The sieve plate plugging structures of host plants	1009:1058	The sieve plate plugging structures of host plants were shown to have different composition in different citrus tissues.
31818905	3	40	theme	cell-to-cell	507:518	arg1	movement					520:527	CLas cell-to-cell movement	502:527	CLas cell-to-cell movement	502:527	However, very little is known about the intracellular mechanisms that take place during CLas cell-to-cell movement.
31818905	4	41	theme	CLas	549:552	arg1	movement					554:561	CLas movement	549:561	CLas movement through phloem pores of sweet orange (Citrus sinensis) and grapefruit (Citrus paradisi)	549:649	Here, we show that CLas movement through phloem pores of sweet orange (Citrus sinensis) and grapefruit (Citrus paradisi) is carried out by the elongated form of the bacteria.
31818905	1	42	dep	Liberibacter	221:232	arg1	Candidatus					210:219	Candidatus	210:219	Candidatus	210:219	Citrus greening or Huanglongbing (HLB) is caused by the phloem-limited intracellular Gram-negative bacterium Candidatus Liberibacter asiaticus (CLas).
31818905	1	42	dep	Liberibacter	221:232	arg1	asiaticus					234:242	Liberibacter asiaticus	221:242	Liberibacter asiaticus (CLas)	221:249	Citrus greening or Huanglongbing (HLB) is caused by the phloem-limited intracellular Gram-negative bacterium Candidatus Liberibacter asiaticus (CLas).
31818905	2	43	theme	callose	353:359	arg1	induction					380:388	callose and phloem protein induction	353:388	callose and phloem protein induction	353:388	HLB-infected citrus phloem cells undergo structural modifications that include cell wall thickening, callose and phloem protein induction, and cellular plugging.
31818905	8	44	theme	plants	1053:1058	arg1	structures					1034:1043	The sieve plate plugging structures	1009:1043	The sieve plate plugging structures of host plants	1009:1058	The sieve plate plugging structures of host plants were shown to have different composition in different citrus tissues.
31818905	6	45	theme	phloem	852:857	arg1	cells					859:863	the phloem cells	848:863	the phloem cells	848:863	CLas cells adhere to the plasma membrane of the phloem cells specifically adjacent to the sieve pores.
31818905	4	46	theme	sweet	587:591	arg1	orange					593:598	sweet orange	587:598	sweet orange (Citrus sinensis)	587:616	Here, we show that CLas movement through phloem pores of sweet orange (Citrus sinensis) and grapefruit (Citrus paradisi) is carried out by the elongated form of the bacteria.
31818905	4	46	theme	sweet	587:591	arg1	sinensis					608:615	Citrus sinensis	601:615	Citrus sinensis	601:615	Here, we show that CLas movement through phloem pores of sweet orange (Citrus sinensis) and grapefruit (Citrus paradisi) is carried out by the elongated form of the bacteria.
31818905	2	47	theme	phloem	365:370	arg1	induction					380:388	callose and phloem protein induction	353:388	callose and phloem protein induction	353:388	HLB-infected citrus phloem cells undergo structural modifications that include cell wall thickening, callose and phloem protein induction, and cellular plugging.
31818905	10	48	theme	dark	1293:1296	arg1	material					1312:1319	dark extracellular material	1293:1319	dark extracellular material	1293:1319	In the roots, pores were surrounded by dark extracellular material, with very little accumulation of callose.
31818905	2	49	theme	structural	293:302	arg1	modifications					304:316	structural modifications	293:316	structural modifications that include cell wall thickening, callose and phloem protein induction, and cellular plugging	293:411	HLB-infected citrus phloem cells undergo structural modifications that include cell wall thickening, callose and phloem protein induction, and cellular plugging.
31818905	8	50	theme	different	1104:1112	arg1	tissues					1121:1127	different citrus tissues	1104:1127	different citrus tissues	1104:1127	The sieve plate plugging structures of host plants were shown to have different composition in different citrus tissues.
31818905	6	51	theme	plasma	829:834	arg1	membrane					836:843	the plasma membrane	825:843	the plasma membrane of the phloem cells	825:863	CLas cells adhere to the plasma membrane of the phloem cells specifically adjacent to the sieve pores.
31818905	13	52	theme	cell-to-cell	1662:1673	arg1	movement					1675:1682	Gram-negative bacterial cell-to-cell movement	1638:1682	Gram-negative bacterial cell-to-cell movement in plant phloem	1638:1698	Our results provide insight into the cellular mechanisms of Gram-negative bacterial cell-to-cell movement in plant phloem.
31818905	10	53	theme	little	1332:1337	arg1	accumulation					1339:1350	very little accumulation	1327:1350	very little accumulation of callose	1327:1361	In the roots, pores were surrounded by dark extracellular material, with very little accumulation of callose.
31818905	8	54	contain	have	1074:1077	arg1	structures					1034:1043	The sieve plate plugging structures	1009:1043	The sieve plate plugging structures of host plants	1009:1058	The sieve plate plugging structures of host plants were shown to have different composition in different citrus tissues.
31818905	8	54	contain	have	1074:1077	arg2	composition					1089:1099	different composition	1079:1099	different composition	1079:1099	The sieve plate plugging structures of host plants were shown to have different composition in different citrus tissues.
31818905	1	55	theme	intracellular	172:184	arg1	bacterium					200:208	the phloem-limited intracellular Gram-negative bacterium	153:208	the phloem-limited intracellular Gram-negative bacterium Candidatus Liberibacter asiaticus (CLas)	153:249	Citrus greening or Huanglongbing (HLB) is caused by the phloem-limited intracellular Gram-negative bacterium Candidatus Liberibacter asiaticus (CLas).
31818905	13	56	from	mechanisms	1624:1633	arg1	phloem					1693:1698	plant phloem	1687:1698	plant phloem	1687:1698	Our results provide insight into the cellular mechanisms of Gram-negative bacterial cell-to-cell movement in plant phloem.
31818905	12	57	theme	phloem	1510:1515	arg1	occlusion					1517:1525	no phloem occlusion	1507:1525	no phloem occlusion	1507:1525	In seed coats, no phloem occlusion was observed, and CLas accumulated to high levels.
31818905	9	58	theme	plugging	1162:1169	arg1	mechanism					1171:1179	the main plugging mechanism	1153:1179	the main plugging mechanism in the HLB-infected flush, where it reduced the open space of the pores	1153:1251	Callose deposition was the main plugging mechanism in the HLB-infected flush, where it reduced the open space of the pores.
31818905	9	58	theme	plugging	1162:1169	arg1	deposition					1138:1147	Callose deposition	1130:1147	Callose deposition	1130:1147	Callose deposition was the main plugging mechanism in the HLB-infected flush, where it reduced the open space of the pores.
31818905	2	59	theme	cell	331:334	arg1	thickening					341:350	cell wall thickening	331:350	cell wall thickening	331:350	HLB-infected citrus phloem cells undergo structural modifications that include cell wall thickening, callose and phloem protein induction, and cellular plugging.
31818905	7	60	theme	element	994:1000	arg1	cells					1002:1006	nucleated nonsieve element cells	975:1006	nucleated nonsieve element cells	975:1006	Remarkably, CLas was present in both mature sieve element cells and nucleated nonsieve element cells.
31818905	6	61	theme	sieve	894:898	arg1	pores					900:904	the sieve pores	890:904	the sieve pores	890:904	CLas cells adhere to the plasma membrane of the phloem cells specifically adjacent to the sieve pores.
31818905	7	62	theme	nucleated	975:983	arg1	cells					1002:1006	nucleated nonsieve element cells	975:1006	nucleated nonsieve element cells	975:1006	Remarkably, CLas was present in both mature sieve element cells and nucleated nonsieve element cells.
31818905	9	63	theme	HLB-infected	1188:1199	arg1	flush					1201:1205	the HLB-infected flush	1184:1205	the HLB-infected flush	1184:1205	Callose deposition was the main plugging mechanism in the HLB-infected flush, where it reduced the open space of the pores.
31818905	7	64	theme	element	957:963	arg1	cells					965:969	mature sieve element cells	944:969	mature sieve element cells	944:969	Remarkably, CLas was present in both mature sieve element cells and nucleated nonsieve element cells.
31818905	11	65	theme	PROTEIN2	1411:1418	arg1	genes					1420:1424	PHLOEM PROTEIN2 genes	1404:1424	PHLOEM PROTEIN2 genes	1404:1424	The expression of CALLOSE SYNTHASE7 and PHLOEM PROTEIN2 genes was upregulated in the shoots, but downregulated in root tissues.
31818905	11	66	theme	root	1478:1481	arg1	tissues					1483:1489	root tissues	1478:1489	root tissues	1478:1489	The expression of CALLOSE SYNTHASE7 and PHLOEM PROTEIN2 genes was upregulated in the shoots, but downregulated in root tissues.
31818905	7	67	theme	mature	944:949	arg1	cells					965:969	mature sieve element cells	944:969	mature sieve element cells	944:969	Remarkably, CLas was present in both mature sieve element cells and nucleated nonsieve element cells.
31818905	2	68	theme	citrus	265:270	arg1	cells					279:283	HLB-infected citrus phloem cells	252:283	HLB-infected citrus phloem cells	252:283	HLB-infected citrus phloem cells undergo structural modifications that include cell wall thickening, callose and phloem protein induction, and cellular plugging.
31818905	7	69	attach	present	928:934	arg2	CLas					919:922	CLas	919:922	CLas	919:922	Remarkably, CLas was present in both mature sieve element cells and nucleated nonsieve element cells.
31818905	7	69	attach	present	928:934	arg1	cells					965:969	mature sieve element cells	944:969	mature sieve element cells	944:969	Remarkably, CLas was present in both mature sieve element cells and nucleated nonsieve element cells.
31818905	7	69	attach	present	928:934	arg1	cells					1002:1006	nucleated nonsieve element cells	975:1006	nucleated nonsieve element cells	975:1006	Remarkably, CLas was present in both mature sieve element cells and nucleated nonsieve element cells.
31818905	0	70	theme	Sink	89:92	arg1	Cells					94:98	Citrus Sink Cells	82:98	Citrus Sink Cells	82:98	Dynamics of Candidatus Liberibacter asiaticus Movement and Sieve-Pore Plugging in Citrus Sink Cells.
31818905	5	71	theme	round	709:713	arg1	large					735:739	large	735:739	large	735:739	The round form of CLas is too large to move, but can change its morphology to enable its movement.
31818905	5	71	theme	round	709:713	arg1	form					715:718	The round form	705:718	The round form of CLas	705:726	The round form of CLas is too large to move, but can change its morphology to enable its movement.
31818905	12	72	located	observed	1531:1538	arg1	coats					1500:1504	seed coats	1495:1504	seed coats	1495:1504	In seed coats, no phloem occlusion was observed, and CLas accumulated to high levels.
31818905	12	72	located	observed	1531:1538	arg2	occlusion					1517:1525	no phloem occlusion	1507:1525	no phloem occlusion	1507:1525	In seed coats, no phloem occlusion was observed, and CLas accumulated to high levels.
31818905	13	73	theme	cellular	1615:1622	arg1	mechanisms					1624:1633	the cellular mechanisms	1611:1633	the cellular mechanisms of Gram-negative bacterial cell-to-cell movement in plant phloem	1611:1698	Our results provide insight into the cellular mechanisms of Gram-negative bacterial cell-to-cell movement in plant phloem.
31818905	3	74	theme	intracellular	454:466	arg1	mechanisms					468:477	the intracellular mechanisms	450:477	the intracellular mechanisms that take place during CLas cell-to-cell movement	450:527	However, very little is known about the intracellular mechanisms that take place during CLas cell-to-cell movement.
31818905	13	75	from	movement	1675:1682	arg1	phloem					1693:1698	plant phloem	1687:1698	plant phloem	1687:1698	Our results provide insight into the cellular mechanisms of Gram-negative bacterial cell-to-cell movement in plant phloem.
31818905	12	76	theme	high	1565:1568	arg1	levels					1570:1575	high levels	1565:1575	high levels	1565:1575	In seed coats, no phloem occlusion was observed, and CLas accumulated to high levels.
31818905	4	77	theme	phloem	571:576	arg1	pores					578:582	phloem pores	571:582	phloem pores of sweet orange (Citrus sinensis) and grapefruit (Citrus paradisi)	571:649	Here, we show that CLas movement through phloem pores of sweet orange (Citrus sinensis) and grapefruit (Citrus paradisi) is carried out by the elongated form of the bacteria.
31818905	11	78	theme	CALLOSE	1382:1388	arg1	SYNTHASE7					1390:1398	CALLOSE SYNTHASE7	1382:1398	CALLOSE SYNTHASE7	1382:1398	The expression of CALLOSE SYNTHASE7 and PHLOEM PROTEIN2 genes was upregulated in the shoots, but downregulated in root tissues.
31818905	2	79	theme	cellular	395:402	arg1	plugging					404:411	cellular plugging	395:411	cellular plugging	395:411	HLB-infected citrus phloem cells undergo structural modifications that include cell wall thickening, callose and phloem protein induction, and cellular plugging.
31818905	0	80	theme	Liberibacter	23:34	arg1	Movement					46:53	Candidatus Liberibacter asiaticus Movement	12:53	Candidatus Liberibacter asiaticus Movement	12:53	Dynamics of Candidatus Liberibacter asiaticus Movement and Sieve-Pore Plugging in Citrus Sink Cells.
31818905	8	81	theme	plate	1019:1023	arg1	structures					1034:1043	The sieve plate plugging structures	1009:1043	The sieve plate plugging structures of host plants	1009:1058	The sieve plate plugging structures of host plants were shown to have different composition in different citrus tissues.
31818905	1	82	theme	Citrus	101:106	arg1	HLB					135:137	HLB	135:137	HLB	135:137	Citrus greening or Huanglongbing (HLB) is caused by the phloem-limited intracellular Gram-negative bacterium Candidatus Liberibacter asiaticus (CLas).
31818905	1	82	theme	Citrus	101:106	arg1	greening					108:115	Citrus greening	101:115	Citrus greening	101:115	Citrus greening or Huanglongbing (HLB) is caused by the phloem-limited intracellular Gram-negative bacterium Candidatus Liberibacter asiaticus (CLas).
31818905	0	83	theme	Movement	46:53	arg1	Dynamics					0:7	Dynamics	0:7	Dynamics of Candidatus Liberibacter asiaticus Movement and Sieve-Pore Plugging in Citrus Sink Cells.	0:99	Dynamics of Candidatus Liberibacter asiaticus Movement and Sieve-Pore Plugging in Citrus Sink Cells.
31818905	0	84	theme	Sieve-Pore	59:68	arg1	Plugging					70:77	Sieve-Pore Plugging	59:77	Sieve-Pore Plugging	59:77	Dynamics of Candidatus Liberibacter asiaticus Movement and Sieve-Pore Plugging in Citrus Sink Cells.
31818905	12	85	theme	seed	1495:1498	arg1	coats					1500:1504	seed coats	1495:1504	seed coats	1495:1504	In seed coats, no phloem occlusion was observed, and CLas accumulated to high levels.
31818905	8	86	theme	host	1048:1051	arg1	plants					1053:1058	host plants	1048:1058	host plants	1048:1058	The sieve plate plugging structures of host plants were shown to have different composition in different citrus tissues.
31818905	6	87	theme	CLas	804:807	arg1	cells					809:813	CLas cells	804:813	CLas cells	804:813	CLas cells adhere to the plasma membrane of the phloem cells specifically adjacent to the sieve pores.
31818905	7	88	from	cells	965:969	arg1	present					928:934	present	928:934	present	928:934	Remarkably, CLas was present in both mature sieve element cells and nucleated nonsieve element cells.
31818905	3	89	theme	CLas	502:505	arg1	movement					520:527	CLas cell-to-cell movement	502:527	CLas cell-to-cell movement	502:527	However, very little is known about the intracellular mechanisms that take place during CLas cell-to-cell movement.
31818905	4	90	theme	grapefruit	622:631	arg1	pores					578:582	phloem pores	571:582	phloem pores of sweet orange (Citrus sinensis) and grapefruit (Citrus paradisi)	571:649	Here, we show that CLas movement through phloem pores of sweet orange (Citrus sinensis) and grapefruit (Citrus paradisi) is carried out by the elongated form of the bacteria.
31818905	7	91	located	present	928:934	arg1	cells					1002:1006	nucleated nonsieve element cells	975:1006	nucleated nonsieve element cells	975:1006	Remarkably, CLas was present in both mature sieve element cells and nucleated nonsieve element cells.
31818905	7	91	located	present	928:934	arg1	cells					965:969	mature sieve element cells	944:969	mature sieve element cells	944:969	Remarkably, CLas was present in both mature sieve element cells and nucleated nonsieve element cells.
31818905	7	91	located	present	928:934	arg2	CLas					919:922	CLas	919:922	CLas	919:922	Remarkably, CLas was present in both mature sieve element cells and nucleated nonsieve element cells.
30813043	8	0	theme	viability	1193:1201	arg1	suppression					1203:1213	cell viability suppression	1188:1213	cell viability suppression	1188:1213	Significant difference in cell viability suppression was observed in in vitro photothermal, chemo-photothermal and chemo-magnetophotothermal killing of cancer cells using SPANs in U87MG cell lines.
30813043	11	1	dep	in	1655:1656	arg1	vivo					1658:1661	vivo	1658:1661	vivo	1658:1661	The in vivo results (both qualitative and quantitative) confirmed enhanced uptake of SPANs in brain after intranasal administration with enhanced nasal and mucus penetration owing to presence of Lf.
30813043	3	2	theme	carboxylic	528:537	arg1	present					545:551	excess carboxylic group present	521:551	excess carboxylic group present on hyaluronic acid (HA)	521:575	Hyaluronic acid was conjugated to amino groups of APTES while lactoferrin (Lf) was directly conjugated to excess carboxylic group present on hyaluronic acid (HA) to form surface modified pH sensitive alloy-drug nanoconjugates (SPANs).
30813043	11	3	theme	intranasal	1757:1766	arg1	administration					1768:1781	intranasal administration	1757:1781	intranasal administration with enhanced nasal and mucus penetration owing to presence of Lf	1757:1847	The in vivo results (both qualitative and quantitative) confirmed enhanced uptake of SPANs in brain after intranasal administration with enhanced nasal and mucus penetration owing to presence of Lf.
30813043	7	4	theme	SPANs	1155:1159	arg1	effect					1145:1150	therapeutic effect	1133:1150	therapeutic effect of SPANs	1133:1159	Owing to leaching of Fe and Pt contents, SPANs demonstrated ability to generate reactive oxygen species (ROS) in U87MG cell line which further enhanced therapeutic effect of SPANs.
30813043	1	5	theme	3-Aminopropyl	294:306	arg1	APTES					326:330	APTES	326:330	APTES	326:330	In the present investigation, FePt alloy nanoparticles were synthesized with controlled size and elemental composition followed by surface modification using (3-Aminopropyl) triethoxysilane (APTES).
30813043	1	5	theme	3-Aminopropyl	294:306	arg1	triethoxysilane					309:323	(3-Aminopropyl) triethoxysilane	293:323	(3-Aminopropyl) triethoxysilane (APTES)	293:331	In the present investigation, FePt alloy nanoparticles were synthesized with controlled size and elemental composition followed by surface modification using (3-Aminopropyl) triethoxysilane (APTES).
30813043	8	6	theme	cancer	1314:1319	arg1	cells					1321:1325	cancer cells	1314:1325	cancer cells using SPANs in U87MG cell lines	1314:1357	Significant difference in cell viability suppression was observed in in vitro photothermal, chemo-photothermal and chemo-magnetophotothermal killing of cancer cells using SPANs in U87MG cell lines.
30813043	3	7	theme	hyaluronic	556:565	arg1	acid					567:570	hyaluronic acid	556:570	hyaluronic acid (HA)	556:575	Hyaluronic acid was conjugated to amino groups of APTES while lactoferrin (Lf) was directly conjugated to excess carboxylic group present on hyaluronic acid (HA) to form surface modified pH sensitive alloy-drug nanoconjugates (SPANs).
30813043	3	7	theme	hyaluronic	556:565	arg1	HA					573:574	HA	573:574	HA	573:574	Hyaluronic acid was conjugated to amino groups of APTES while lactoferrin (Lf) was directly conjugated to excess carboxylic group present on hyaluronic acid (HA) to form surface modified pH sensitive alloy-drug nanoconjugates (SPANs).
30813043	9	8	theme	SPANs	1536:1540	arg1	exposure					1524:1531	DUAL-mode exposure	1514:1531	DUAL-mode exposure of SPANs	1514:1540	Significant difference in heating ability and cell cytotoxicity of SPANs in comparison to alternative magnetic field and NIR irradiation was observed for DUAL-mode exposure of SPANs.
30813043	3	9	dep	nanoconjugates	626:639	arg1	SPANs					642:646	SPANs	642:646	SPANs	642:646	Hyaluronic acid was conjugated to amino groups of APTES while lactoferrin (Lf) was directly conjugated to excess carboxylic group present on hyaluronic acid (HA) to form surface modified pH sensitive alloy-drug nanoconjugates (SPANs).
30813043	4	10	theme	multifunctional	654:668	arg1	nanoconjugates					670:683	The multifunctional nanoconjugates	650:683	The multifunctional nanoconjugates	650:683	The multifunctional nanoconjugates were characterized and evaluated using extensive in vitro and in vivo techniques.
30813043	7	11	theme	cell	1100:1103	arg1	line					1105:1108	U87MG cell line	1094:1108	U87MG cell line which further enhanced therapeutic effect of SPANs	1094:1159	Owing to leaching of Fe and Pt contents, SPANs demonstrated ability to generate reactive oxygen species (ROS) in U87MG cell line which further enhanced therapeutic effect of SPANs.
30813043	3	12	theme	sensitive	605:613	arg1	nanoconjugates					626:639	surface modified pH sensitive alloy-drug nanoconjugates	585:639	surface modified pH sensitive alloy-drug nanoconjugates (SPANs)	585:647	Hyaluronic acid was conjugated to amino groups of APTES while lactoferrin (Lf) was directly conjugated to excess carboxylic group present on hyaluronic acid (HA) to form surface modified pH sensitive alloy-drug nanoconjugates (SPANs).
30813043	7	13	theme	oxygen	1070:1075	arg1	ROS					1086:1088	ROS	1086:1088	ROS	1086:1088	Owing to leaching of Fe and Pt contents, SPANs demonstrated ability to generate reactive oxygen species (ROS) in U87MG cell line which further enhanced therapeutic effect of SPANs.
30813043	7	13	theme	oxygen	1070:1075	arg1	species					1077:1083	reactive oxygen species	1061:1083	reactive oxygen species (ROS)	1061:1089	Owing to leaching of Fe and Pt contents, SPANs demonstrated ability to generate reactive oxygen species (ROS) in U87MG cell line which further enhanced therapeutic effect of SPANs.
30813043	1	14	theme	elemental	232:240	arg1	composition					242:252	elemental composition	232:252	elemental composition	232:252	In the present investigation, FePt alloy nanoparticles were synthesized with controlled size and elemental composition followed by surface modification using (3-Aminopropyl) triethoxysilane (APTES).
30813043	9	15	dep	field	1471:1475	arg1	comparison					1436:1445	comparison	1436:1445	comparison	1436:1445	Significant difference in heating ability and cell cytotoxicity of SPANs in comparison to alternative magnetic field and NIR irradiation was observed for DUAL-mode exposure of SPANs.
30813043	3	16	theme	modified	593:600	arg1	nanoconjugates					626:639	surface modified pH sensitive alloy-drug nanoconjugates	585:639	surface modified pH sensitive alloy-drug nanoconjugates (SPANs)	585:647	Hyaluronic acid was conjugated to amino groups of APTES while lactoferrin (Lf) was directly conjugated to excess carboxylic group present on hyaluronic acid (HA) to form surface modified pH sensitive alloy-drug nanoconjugates (SPANs).
30813043	11	17	theme	Lf	1846:1847	arg1	presence					1834:1841	presence	1834:1841	presence of Lf	1834:1847	The in vivo results (both qualitative and quantitative) confirmed enhanced uptake of SPANs in brain after intranasal administration with enhanced nasal and mucus penetration owing to presence of Lf.
30813043	1	18	theme	present	142:148	arg1	investigation					150:162	the present investigation	138:162	the present investigation	138:162	In the present investigation, FePt alloy nanoparticles were synthesized with controlled size and elemental composition followed by surface modification using (3-Aminopropyl) triethoxysilane (APTES).
30813043	5	19	theme	excellent	799:807	arg1	ability					817:823	excellent heating ability	799:823	excellent heating ability	799:823	The nanoconjugates demonstrated excellent heating ability on exposure to alternating magnetic field and near-infrared laser irradiation.
30813043	4	20	theme	in	734:735	arg1	techniques					755:764	extensive in vitro and in vivo techniques	724:764	extensive in vitro and in vivo techniques	724:764	The multifunctional nanoconjugates were characterized and evaluated using extensive in vitro and in vivo techniques.
30813043	0	21	theme	glioblastoma	91:102	arg1	therapy					80:86	multimodal therapy	69:86	multimodal therapy of glioblastoma	69:102	Hyaluronic acid tethered pH-responsive alloy-drug nanoconjugates for multimodal therapy of glioblastoma: An intranasal route approach.
30813043	9	22	theme	Significant	1360:1370	arg1	difference					1372:1381	Significant difference	1360:1381	Significant difference in heating ability and cell cytotoxicity of SPANs in comparison to alternative magnetic field and NIR irradiation	1360:1495	Significant difference in heating ability and cell cytotoxicity of SPANs in comparison to alternative magnetic field and NIR irradiation was observed for DUAL-mode exposure of SPANs.
30813043	7	23	theme	contents	1012:1019	arg1	leaching					990:997	leaching	990:997	leaching of Fe and Pt contents	990:1019	Owing to leaching of Fe and Pt contents, SPANs demonstrated ability to generate reactive oxygen species (ROS) in U87MG cell line which further enhanced therapeutic effect of SPANs.
30813043	1	24	theme	alloy	170:174	arg1	nanoparticles					176:188	FePt alloy nanoparticles	165:188	FePt alloy nanoparticles	165:188	In the present investigation, FePt alloy nanoparticles were synthesized with controlled size and elemental composition followed by surface modification using (3-Aminopropyl) triethoxysilane (APTES).
30813043	3	25	theme	APTES	465:469	arg1	APTES					465:469	APTES	465:469	APTES	465:469	Hyaluronic acid was conjugated to amino groups of APTES while lactoferrin (Lf) was directly conjugated to excess carboxylic group present on hyaluronic acid (HA) to form surface modified pH sensitive alloy-drug nanoconjugates (SPANs).
30813043	3	25	theme	APTES	465:469	arg1	groups					455:460	amino groups	449:460	amino groups of APTES	449:469	Hyaluronic acid was conjugated to amino groups of APTES while lactoferrin (Lf) was directly conjugated to excess carboxylic group present on hyaluronic acid (HA) to form surface modified pH sensitive alloy-drug nanoconjugates (SPANs).
30813043	0	26	theme	route	119:123	arg1	approach					125:132	An intranasal route approach	105:132	An intranasal route approach	105:132	Hyaluronic acid tethered pH-responsive alloy-drug nanoconjugates for multimodal therapy of glioblastoma: An intranasal route approach.
30813043	3	27	theme	Hyaluronic	415:424	arg1	acid					426:429	Hyaluronic acid	415:429	Hyaluronic acid	415:429	Hyaluronic acid was conjugated to amino groups of APTES while lactoferrin (Lf) was directly conjugated to excess carboxylic group present on hyaluronic acid (HA) to form surface modified pH sensitive alloy-drug nanoconjugates (SPANs).
30813043	0	28	theme	Hyaluronic	0:9	arg1	acid					11:14	Hyaluronic acid	0:14	Hyaluronic acid	0:14	Hyaluronic acid tethered pH-responsive alloy-drug nanoconjugates for multimodal therapy of glioblastoma: An intranasal route approach.
30813043	9	29	theme	cell	1406:1409	arg1	cytotoxicity					1411:1422	cell cytotoxicity	1406:1422	cell cytotoxicity	1406:1422	Significant difference in heating ability and cell cytotoxicity of SPANs in comparison to alternative magnetic field and NIR irradiation was observed for DUAL-mode exposure of SPANs.
30813043	4	30	dep	in	747:748	arg1	vivo					750:753	vivo	750:753	vivo	750:753	The multifunctional nanoconjugates were characterized and evaluated using extensive in vitro and in vivo techniques.
30813043	8	31	located	observed	1219:1226	arg2	difference					1174:1183	Significant difference	1162:1183	Significant difference in cell viability suppression	1162:1213	Significant difference in cell viability suppression was observed in in vitro photothermal, chemo-photothermal and chemo-magnetophotothermal killing of cancer cells using SPANs in U87MG cell lines.
30813043	8	31	located	observed	1219:1226	arg1	killing					1303:1309	in vitro photothermal, chemo-photothermal and chemo-magnetophotothermal killing	1231:1309	in vitro photothermal, chemo-photothermal and chemo-magnetophotothermal killing of cancer cells using SPANs in U87MG cell lines	1231:1357	Significant difference in cell viability suppression was observed in in vitro photothermal, chemo-photothermal and chemo-magnetophotothermal killing of cancer cells using SPANs in U87MG cell lines.
30813043	5	32	theme	near-infrared	871:883	arg1	irradiation					891:901	near-infrared laser irradiation	871:901	near-infrared laser irradiation	871:901	The nanoconjugates demonstrated excellent heating ability on exposure to alternating magnetic field and near-infrared laser irradiation.
30813043	6	33	theme	lysozome	935:942	arg1	microenvironment					915:930	The acidic microenvironment	904:930	The acidic microenvironment of lysozome	904:942	The acidic microenvironment of lysozome triggered release of LND from SPANs.
30813043	11	34	from	uptake	1726:1731	arg1	brain					1745:1749	brain	1745:1749	brain	1745:1749	The in vivo results (both qualitative and quantitative) confirmed enhanced uptake of SPANs in brain after intranasal administration with enhanced nasal and mucus penetration owing to presence of Lf.
30813043	10	35	theme	internalization	1567:1581	arg1	study					1583:1587	cellular internalization study	1558:1587	cellular internalization study	1558:1587	The results of cellular internalization study showed efficient internalization of SPANs inside U87MG cells.
30813043	0	36	theme	alloy-drug	39:48	arg1	nanoconjugates					50:63	pH-responsive alloy-drug nanoconjugates	25:63	pH-responsive alloy-drug nanoconjugates for multimodal therapy of glioblastoma	25:102	Hyaluronic acid tethered pH-responsive alloy-drug nanoconjugates for multimodal therapy of glioblastoma: An intranasal route approach.
30813043	12	37	theme	due	1909:1911	arg1	ECM					1895:1897	ECM	1895:1897	ECM	1895:1897	No significant interaction was observed with ECM and mucin due to presence of carboxyl group on SPANs.
30813043	10	38	theme	SPANs	1625:1629	arg1	internalization					1606:1620	efficient internalization	1596:1620	efficient internalization of SPANs inside U87MG cells	1596:1648	The results of cellular internalization study showed efficient internalization of SPANs inside U87MG cells.
30813043	11	39	theme	enhanced	1788:1795	arg1	penetration					1813:1823	enhanced nasal and mucus penetration	1788:1823	enhanced nasal and mucus penetration owing to presence of Lf	1788:1847	The in vivo results (both qualitative and quantitative) confirmed enhanced uptake of SPANs in brain after intranasal administration with enhanced nasal and mucus penetration owing to presence of Lf.
30813043	5	40	theme	alternating	840:850	arg1	field					861:865	alternating magnetic field	840:865	alternating magnetic field	840:865	The nanoconjugates demonstrated excellent heating ability on exposure to alternating magnetic field and near-infrared laser irradiation.
30813043	9	41	theme	magnetic	1462:1469	arg1	field					1471:1475	alternative magnetic field	1450:1475	alternative magnetic field	1450:1475	Significant difference in heating ability and cell cytotoxicity of SPANs in comparison to alternative magnetic field and NIR irradiation was observed for DUAL-mode exposure of SPANs.
30813043	12	42	theme	group	1937:1941	arg1	presence					1916:1923	presence	1916:1923	presence of carboxyl group on SPANs	1916:1950	No significant interaction was observed with ECM and mucin due to presence of carboxyl group on SPANs.
30813043	0	43	dep	tethered	16:23	arg1	approach					125:132	An intranasal route approach	105:132	An intranasal route approach	105:132	Hyaluronic acid tethered pH-responsive alloy-drug nanoconjugates for multimodal therapy of glioblastoma: An intranasal route approach.
30813043	6	44	from	SPANs	974:978	arg1	release					954:960	release	954:960	release of LND from SPANs	954:978	The acidic microenvironment of lysozome triggered release of LND from SPANs.
30813043	11	45	theme	enhanced	1717:1724	arg1	uptake					1726:1731	enhanced uptake	1717:1731	enhanced uptake of SPANs in brain	1717:1749	The in vivo results (both qualitative and quantitative) confirmed enhanced uptake of SPANs in brain after intranasal administration with enhanced nasal and mucus penetration owing to presence of Lf.
30813043	2	46	theme	hydrazone	396:404	arg1	bonding					406:412	pH sensitive hydrazone bonding	383:412	pH sensitive hydrazone bonding	383:412	Lenalidomide was covalently bound to FePt-NH2 by pH sensitive hydrazone bonding.
30813043	11	47	with	administration	1768:1781	arg1	penetration					1813:1823	enhanced nasal and mucus penetration	1788:1823	enhanced nasal and mucus penetration owing to presence of Lf	1788:1847	The in vivo results (both qualitative and quantitative) confirmed enhanced uptake of SPANs in brain after intranasal administration with enhanced nasal and mucus penetration owing to presence of Lf.
30813043	10	48	theme	U87MG	1638:1642	arg1	cells					1644:1648	U87MG cells	1638:1648	U87MG cells	1638:1648	The results of cellular internalization study showed efficient internalization of SPANs inside U87MG cells.
30813043	3	49	theme	excess	521:526	arg1	present					545:551	excess carboxylic group present	521:551	excess carboxylic group present on hyaluronic acid (HA)	521:575	Hyaluronic acid was conjugated to amino groups of APTES while lactoferrin (Lf) was directly conjugated to excess carboxylic group present on hyaluronic acid (HA) to form surface modified pH sensitive alloy-drug nanoconjugates (SPANs).
30813043	8	50	theme	cell	1188:1191	arg1	suppression					1203:1213	cell viability suppression	1188:1213	cell viability suppression	1188:1213	Significant difference in cell viability suppression was observed in in vitro photothermal, chemo-photothermal and chemo-magnetophotothermal killing of cancer cells using SPANs in U87MG cell lines.
30813043	12	51	theme	significant	1853:1863	arg1	interaction					1865:1875	No significant interaction	1850:1875	No significant interaction	1850:1875	No significant interaction was observed with ECM and mucin due to presence of carboxyl group on SPANs.
30813043	6	52	theme	acidic	908:913	arg1	microenvironment					915:930	The acidic microenvironment	904:930	The acidic microenvironment of lysozome	904:942	The acidic microenvironment of lysozome triggered release of LND from SPANs.
30813043	6	53	theme	LND	965:967	arg1	release					954:960	release	954:960	release of LND from SPANs	954:978	The acidic microenvironment of lysozome triggered release of LND from SPANs.
30813043	7	54	theme	therapeutic	1133:1143	arg1	effect					1145:1150	therapeutic effect	1133:1150	therapeutic effect of SPANs	1133:1159	Owing to leaching of Fe and Pt contents, SPANs demonstrated ability to generate reactive oxygen species (ROS) in U87MG cell line which further enhanced therapeutic effect of SPANs.
30813043	3	55	from	present	545:551	arg1	acid					567:570	hyaluronic acid	556:570	hyaluronic acid (HA)	556:575	Hyaluronic acid was conjugated to amino groups of APTES while lactoferrin (Lf) was directly conjugated to excess carboxylic group present on hyaluronic acid (HA) to form surface modified pH sensitive alloy-drug nanoconjugates (SPANs).
30813043	3	55	from	present	545:551	arg1	HA					573:574	HA	573:574	HA	573:574	Hyaluronic acid was conjugated to amino groups of APTES while lactoferrin (Lf) was directly conjugated to excess carboxylic group present on hyaluronic acid (HA) to form surface modified pH sensitive alloy-drug nanoconjugates (SPANs).
30813043	9	56	theme	DUAL-mode	1514:1522	arg1	exposure					1524:1531	DUAL-mode exposure	1514:1531	DUAL-mode exposure of SPANs	1514:1540	Significant difference in heating ability and cell cytotoxicity of SPANs in comparison to alternative magnetic field and NIR irradiation was observed for DUAL-mode exposure of SPANs.
30813043	8	57	theme	in	1231:1232	arg1	killing					1303:1309	in vitro photothermal, chemo-photothermal and chemo-magnetophotothermal killing	1231:1309	in vitro photothermal, chemo-photothermal and chemo-magnetophotothermal killing of cancer cells using SPANs in U87MG cell lines	1231:1357	Significant difference in cell viability suppression was observed in in vitro photothermal, chemo-photothermal and chemo-magnetophotothermal killing of cancer cells using SPANs in U87MG cell lines.
30813043	8	58	theme	cells	1321:1325	arg1	killing					1303:1309	in vitro photothermal, chemo-photothermal and chemo-magnetophotothermal killing	1231:1309	in vitro photothermal, chemo-photothermal and chemo-magnetophotothermal killing of cancer cells using SPANs in U87MG cell lines	1231:1357	Significant difference in cell viability suppression was observed in in vitro photothermal, chemo-photothermal and chemo-magnetophotothermal killing of cancer cells using SPANs in U87MG cell lines.
30813043	11	59	theme	in	1655:1656	arg1	results					1663:1669	The in vivo results	1651:1669	The in vivo results (both qualitative and quantitative)	1651:1705	The in vivo results (both qualitative and quantitative) confirmed enhanced uptake of SPANs in brain after intranasal administration with enhanced nasal and mucus penetration owing to presence of Lf.
30813043	1	60	theme	controlled	212:221	arg1	size					223:226	controlled size	212:226	controlled size	212:226	In the present investigation, FePt alloy nanoparticles were synthesized with controlled size and elemental composition followed by surface modification using (3-Aminopropyl) triethoxysilane (APTES).
30813043	8	61	theme	photothermal	1240:1251	arg1	killing					1303:1309	in vitro photothermal, chemo-photothermal and chemo-magnetophotothermal killing	1231:1309	in vitro photothermal, chemo-photothermal and chemo-magnetophotothermal killing of cancer cells using SPANs in U87MG cell lines	1231:1357	Significant difference in cell viability suppression was observed in in vitro photothermal, chemo-photothermal and chemo-magnetophotothermal killing of cancer cells using SPANs in U87MG cell lines.
30813043	11	62	from	brain	1745:1749	arg1	uptake					1726:1731	enhanced uptake	1717:1731	enhanced uptake of SPANs in brain	1717:1749	The in vivo results (both qualitative and quantitative) confirmed enhanced uptake of SPANs in brain after intranasal administration with enhanced nasal and mucus penetration owing to presence of Lf.
30813043	7	63	theme	U87MG	1094:1098	arg1	line					1105:1108	U87MG cell line	1094:1108	U87MG cell line which further enhanced therapeutic effect of SPANs	1094:1159	Owing to leaching of Fe and Pt contents, SPANs demonstrated ability to generate reactive oxygen species (ROS) in U87MG cell line which further enhanced therapeutic effect of SPANs.
30813043	3	64	theme	pH	602:603	arg1	nanoconjugates					626:639	surface modified pH sensitive alloy-drug nanoconjugates	585:639	surface modified pH sensitive alloy-drug nanoconjugates (SPANs)	585:647	Hyaluronic acid was conjugated to amino groups of APTES while lactoferrin (Lf) was directly conjugated to excess carboxylic group present on hyaluronic acid (HA) to form surface modified pH sensitive alloy-drug nanoconjugates (SPANs).
30813043	9	65	from	difference	1372:1381	arg1	ability					1394:1400	heating ability	1386:1400	heating ability	1386:1400	Significant difference in heating ability and cell cytotoxicity of SPANs in comparison to alternative magnetic field and NIR irradiation was observed for DUAL-mode exposure of SPANs.
30813043	9	65	from	difference	1372:1381	arg1	cytotoxicity					1411:1422	cell cytotoxicity	1406:1422	cell cytotoxicity	1406:1422	Significant difference in heating ability and cell cytotoxicity of SPANs in comparison to alternative magnetic field and NIR irradiation was observed for DUAL-mode exposure of SPANs.
30813043	8	66	theme	U87MG	1342:1346	arg1	lines					1353:1357	U87MG cell lines	1342:1357	U87MG cell lines	1342:1357	Significant difference in cell viability suppression was observed in in vitro photothermal, chemo-photothermal and chemo-magnetophotothermal killing of cancer cells using SPANs in U87MG cell lines.
30813043	8	67	theme	chemo-photothermal	1254:1271	arg1	killing					1303:1309	in vitro photothermal, chemo-photothermal and chemo-magnetophotothermal killing	1231:1309	in vitro photothermal, chemo-photothermal and chemo-magnetophotothermal killing of cancer cells using SPANs in U87MG cell lines	1231:1357	Significant difference in cell viability suppression was observed in in vitro photothermal, chemo-photothermal and chemo-magnetophotothermal killing of cancer cells using SPANs in U87MG cell lines.
30813043	3	68	theme	surface	585:591	arg1	nanoconjugates					626:639	surface modified pH sensitive alloy-drug nanoconjugates	585:639	surface modified pH sensitive alloy-drug nanoconjugates (SPANs)	585:647	Hyaluronic acid was conjugated to amino groups of APTES while lactoferrin (Lf) was directly conjugated to excess carboxylic group present on hyaluronic acid (HA) to form surface modified pH sensitive alloy-drug nanoconjugates (SPANs).
30813043	8	69	theme	chemo-magnetophotothermal	1277:1301	arg1	killing					1303:1309	in vitro photothermal, chemo-photothermal and chemo-magnetophotothermal killing	1231:1309	in vitro photothermal, chemo-photothermal and chemo-magnetophotothermal killing of cancer cells using SPANs in U87MG cell lines	1231:1357	Significant difference in cell viability suppression was observed in in vitro photothermal, chemo-photothermal and chemo-magnetophotothermal killing of cancer cells using SPANs in U87MG cell lines.
30813043	7	70	theme	reactive	1061:1068	arg1	ROS					1086:1088	ROS	1086:1088	ROS	1086:1088	Owing to leaching of Fe and Pt contents, SPANs demonstrated ability to generate reactive oxygen species (ROS) in U87MG cell line which further enhanced therapeutic effect of SPANs.
30813043	7	70	theme	reactive	1061:1068	arg1	species					1077:1083	reactive oxygen species	1061:1083	reactive oxygen species (ROS)	1061:1089	Owing to leaching of Fe and Pt contents, SPANs demonstrated ability to generate reactive oxygen species (ROS) in U87MG cell line which further enhanced therapeutic effect of SPANs.
30813043	4	71	theme	in	747:748	arg1	techniques					755:764	extensive in vitro and in vivo techniques	724:764	extensive in vitro and in vivo techniques	724:764	The multifunctional nanoconjugates were characterized and evaluated using extensive in vitro and in vivo techniques.
30813043	3	72	theme	alloy-drug	615:624	arg1	nanoconjugates					626:639	surface modified pH sensitive alloy-drug nanoconjugates	585:639	surface modified pH sensitive alloy-drug nanoconjugates (SPANs)	585:647	Hyaluronic acid was conjugated to amino groups of APTES while lactoferrin (Lf) was directly conjugated to excess carboxylic group present on hyaluronic acid (HA) to form surface modified pH sensitive alloy-drug nanoconjugates (SPANs).
30813043	5	73	theme	laser	885:889	arg1	irradiation					891:901	near-infrared laser irradiation	871:901	near-infrared laser irradiation	871:901	The nanoconjugates demonstrated excellent heating ability on exposure to alternating magnetic field and near-infrared laser irradiation.
30813043	5	74	theme	heating	809:815	arg1	ability					817:823	excellent heating ability	799:823	excellent heating ability	799:823	The nanoconjugates demonstrated excellent heating ability on exposure to alternating magnetic field and near-infrared laser irradiation.
30813043	8	75	dep	in	1231:1232	arg1	vitro					1234:1238	vitro	1234:1238	vitro	1234:1238	Significant difference in cell viability suppression was observed in in vitro photothermal, chemo-photothermal and chemo-magnetophotothermal killing of cancer cells using SPANs in U87MG cell lines.
30813043	1	76	theme	FePt	165:168	arg1	nanoparticles					176:188	FePt alloy nanoparticles	165:188	FePt alloy nanoparticles	165:188	In the present investigation, FePt alloy nanoparticles were synthesized with controlled size and elemental composition followed by surface modification using (3-Aminopropyl) triethoxysilane (APTES).
30813043	4	77	theme	extensive	724:732	arg1	techniques					755:764	extensive in vitro and in vivo techniques	724:764	extensive in vitro and in vivo techniques	724:764	The multifunctional nanoconjugates were characterized and evaluated using extensive in vitro and in vivo techniques.
30813043	0	78	theme	intranasal	108:117	arg1	approach					125:132	An intranasal route approach	105:132	An intranasal route approach	105:132	Hyaluronic acid tethered pH-responsive alloy-drug nanoconjugates for multimodal therapy of glioblastoma: An intranasal route approach.
30813043	4	79	dep	in	734:735	arg1	vitro					737:741	vitro	737:741	vitro	737:741	The multifunctional nanoconjugates were characterized and evaluated using extensive in vitro and in vivo techniques.
30813043	9	80	theme	heating	1386:1392	arg1	ability					1394:1400	heating ability	1386:1400	heating ability	1386:1400	Significant difference in heating ability and cell cytotoxicity of SPANs in comparison to alternative magnetic field and NIR irradiation was observed for DUAL-mode exposure of SPANs.
30813043	7	81	theme	Pt	1009:1010	arg1	contents					1012:1019	Fe and Pt contents	1002:1019	Fe and Pt contents	1002:1019	Owing to leaching of Fe and Pt contents, SPANs demonstrated ability to generate reactive oxygen species (ROS) in U87MG cell line which further enhanced therapeutic effect of SPANs.
30813043	5	82	theme	magnetic	852:859	arg1	field					861:865	alternating magnetic field	840:865	alternating magnetic field	840:865	The nanoconjugates demonstrated excellent heating ability on exposure to alternating magnetic field and near-infrared laser irradiation.
30813043	12	83	attach	presence	1916:1923	arg1	SPANs					1946:1950	SPANs	1946:1950	SPANs	1946:1950	No significant interaction was observed with ECM and mucin due to presence of carboxyl group on SPANs.
30813043	12	83	attach	presence	1916:1923	arg2	group					1937:1941	carboxyl group	1928:1941	carboxyl group	1928:1941	No significant interaction was observed with ECM and mucin due to presence of carboxyl group on SPANs.
30813043	7	84	theme	Fe	1002:1003	arg1	contents					1012:1019	Fe and Pt contents	1002:1019	Fe and Pt contents	1002:1019	Owing to leaching of Fe and Pt contents, SPANs demonstrated ability to generate reactive oxygen species (ROS) in U87MG cell line which further enhanced therapeutic effect of SPANs.
30813043	0	85	theme	pH-responsive	25:37	arg1	nanoconjugates					50:63	pH-responsive alloy-drug nanoconjugates	25:63	pH-responsive alloy-drug nanoconjugates for multimodal therapy of glioblastoma	25:102	Hyaluronic acid tethered pH-responsive alloy-drug nanoconjugates for multimodal therapy of glioblastoma: An intranasal route approach.
30813043	10	86	theme	study	1583:1587	arg1	results					1547:1553	The results	1543:1553	The results of cellular internalization study	1543:1587	The results of cellular internalization study showed efficient internalization of SPANs inside U87MG cells.
30813043	11	87	theme	nasal	1797:1801	arg1	penetration					1813:1823	enhanced nasal and mucus penetration	1788:1823	enhanced nasal and mucus penetration owing to presence of Lf	1788:1847	The in vivo results (both qualitative and quantitative) confirmed enhanced uptake of SPANs in brain after intranasal administration with enhanced nasal and mucus penetration owing to presence of Lf.
30813043	3	88	theme	group	539:543	arg1	present					545:551	excess carboxylic group present	521:551	excess carboxylic group present on hyaluronic acid (HA)	521:575	Hyaluronic acid was conjugated to amino groups of APTES while lactoferrin (Lf) was directly conjugated to excess carboxylic group present on hyaluronic acid (HA) to form surface modified pH sensitive alloy-drug nanoconjugates (SPANs).
30813043	8	89	theme	cell	1348:1351	arg1	lines					1353:1357	U87MG cell lines	1342:1357	U87MG cell lines	1342:1357	Significant difference in cell viability suppression was observed in in vitro photothermal, chemo-photothermal and chemo-magnetophotothermal killing of cancer cells using SPANs in U87MG cell lines.
30813043	3	90	theme	amino	449:453	arg1	APTES					465:469	APTES	465:469	APTES	465:469	Hyaluronic acid was conjugated to amino groups of APTES while lactoferrin (Lf) was directly conjugated to excess carboxylic group present on hyaluronic acid (HA) to form surface modified pH sensitive alloy-drug nanoconjugates (SPANs).
30813043	3	90	theme	amino	449:453	arg1	groups					455:460	amino groups	449:460	amino groups of APTES	449:469	Hyaluronic acid was conjugated to amino groups of APTES while lactoferrin (Lf) was directly conjugated to excess carboxylic group present on hyaluronic acid (HA) to form surface modified pH sensitive alloy-drug nanoconjugates (SPANs).
30813043	10	91	theme	cellular	1558:1565	arg1	internalization					1567:1581	cellular internalization	1558:1581	cellular internalization study	1558:1587	The results of cellular internalization study showed efficient internalization of SPANs inside U87MG cells.
30813043	11	92	theme	qualitative	1677:1687	arg1	results					1663:1669	The in vivo results	1651:1669	The in vivo results (both qualitative and quantitative)	1651:1705	The in vivo results (both qualitative and quantitative) confirmed enhanced uptake of SPANs in brain after intranasal administration with enhanced nasal and mucus penetration owing to presence of Lf.
30813043	0	93	theme	multimodal	69:78	arg1	therapy					80:86	multimodal therapy	69:86	multimodal therapy of glioblastoma	69:102	Hyaluronic acid tethered pH-responsive alloy-drug nanoconjugates for multimodal therapy of glioblastoma: An intranasal route approach.
30813043	12	94	theme	carboxyl	1928:1935	arg1	group					1937:1941	carboxyl group	1928:1941	carboxyl group	1928:1941	No significant interaction was observed with ECM and mucin due to presence of carboxyl group on SPANs.
30813043	8	95	from	difference	1174:1183	arg1	suppression					1203:1213	cell viability suppression	1188:1213	cell viability suppression	1188:1213	Significant difference in cell viability suppression was observed in in vitro photothermal, chemo-photothermal and chemo-magnetophotothermal killing of cancer cells using SPANs in U87MG cell lines.
30813043	12	96	from	presence	1916:1923	arg1	SPANs					1946:1950	SPANs	1946:1950	SPANs	1946:1950	No significant interaction was observed with ECM and mucin due to presence of carboxyl group on SPANs.
30813043	9	97	theme	alternative	1450:1460	arg1	field					1471:1475	alternative magnetic field	1450:1475	alternative magnetic field	1450:1475	Significant difference in heating ability and cell cytotoxicity of SPANs in comparison to alternative magnetic field and NIR irradiation was observed for DUAL-mode exposure of SPANs.
30813043	8	98	theme	Significant	1162:1172	arg1	difference					1174:1183	Significant difference	1162:1183	Significant difference in cell viability suppression	1162:1213	Significant difference in cell viability suppression was observed in in vitro photothermal, chemo-photothermal and chemo-magnetophotothermal killing of cancer cells using SPANs in U87MG cell lines.
30813043	10	99	theme	efficient	1596:1604	arg1	internalization					1606:1620	efficient internalization	1596:1620	efficient internalization of SPANs inside U87MG cells	1596:1648	The results of cellular internalization study showed efficient internalization of SPANs inside U87MG cells.
30813043	11	100	theme	mucus	1807:1811	arg1	penetration					1813:1823	enhanced nasal and mucus penetration	1788:1823	enhanced nasal and mucus penetration owing to presence of Lf	1788:1847	The in vivo results (both qualitative and quantitative) confirmed enhanced uptake of SPANs in brain after intranasal administration with enhanced nasal and mucus penetration owing to presence of Lf.
30813043	11	101	theme	SPANs	1736:1740	arg1	uptake					1726:1731	enhanced uptake	1717:1731	enhanced uptake of SPANs in brain	1717:1749	The in vivo results (both qualitative and quantitative) confirmed enhanced uptake of SPANs in brain after intranasal administration with enhanced nasal and mucus penetration owing to presence of Lf.
30813043	1	102	theme	surface	266:272	arg1	modification					274:285	surface modification	266:285	surface modification using (3-Aminopropyl) triethoxysilane (APTES)	266:331	In the present investigation, FePt alloy nanoparticles were synthesized with controlled size and elemental composition followed by surface modification using (3-Aminopropyl) triethoxysilane (APTES).
30813043	11	103	theme	quantitative	1693:1704	arg1	results					1663:1669	The in vivo results	1651:1669	The in vivo results (both qualitative and quantitative)	1651:1705	The in vivo results (both qualitative and quantitative) confirmed enhanced uptake of SPANs in brain after intranasal administration with enhanced nasal and mucus penetration owing to presence of Lf.
30813043	2	104	theme	sensitive	386:394	arg1	bonding					406:412	pH sensitive hydrazone bonding	383:412	pH sensitive hydrazone bonding	383:412	Lenalidomide was covalently bound to FePt-NH2 by pH sensitive hydrazone bonding.
30813043	9	105	theme	NIR	1481:1483	arg1	irradiation					1485:1495	NIR irradiation	1481:1495	NIR irradiation	1481:1495	Significant difference in heating ability and cell cytotoxicity of SPANs in comparison to alternative magnetic field and NIR irradiation was observed for DUAL-mode exposure of SPANs.
31812816	3	0	theme	excellent	766:774	arg1	%					797:797	> 98%	793:797	> 98%	793:797	Despite the accumulation of salts, both NF-MBRs at SRT of 30 and 60 days, achieved (i) similar biodegradation efficiency; (ii) excellent organic removal (> 97%); and (iii) excellent ammonia removal (> 98%).
31812816	3	0	theme	excellent	766:774	arg1	removal					784:790	(iii) excellent ammonia removal	760:790	(iii) excellent ammonia removal (> 98%)	760:798	Despite the accumulation of salts, both NF-MBRs at SRT of 30 and 60 days, achieved (i) similar biodegradation efficiency; (ii) excellent organic removal (> 97%); and (iii) excellent ammonia removal (> 98%).
31812816	0	1	theme	water	145:149	arg1	reclamation					151:161	water reclamation	145:161	water reclamation	145:161	Impact of salt accumulation in the bioreactor on the performance of nanofiltration membrane bioreactor (NF-MBR)+Reverse osmosis (RO) process for water reclamation.
31812816	1	2	theme	retention	226:234	arg1	SRT					242:244	SRT	242:244	SRT	242:244	The impacts of salt accumulation, through adjusting the solid retention time (SRT), in the bioreactor on the bioprocess as well as membrane performance of a high retention nanofiltration membrane bioreactor (NF-MBR) and subsequent reverse osmosis (RO) process for water reclamation are addressed in this study.
31812816	1	2	theme	retention	226:234	arg1	time					236:239	the solid retention time	216:239	the solid retention time (SRT)	216:245	The impacts of salt accumulation, through adjusting the solid retention time (SRT), in the bioreactor on the bioprocess as well as membrane performance of a high retention nanofiltration membrane bioreactor (NF-MBR) and subsequent reverse osmosis (RO) process for water reclamation are addressed in this study.
31812816	1	3	dep	bioreactor	360:369	arg1	process					416:422	process	416:422	process	416:422	The impacts of salt accumulation, through adjusting the solid retention time (SRT), in the bioreactor on the bioprocess as well as membrane performance of a high retention nanofiltration membrane bioreactor (NF-MBR) and subsequent reverse osmosis (RO) process for water reclamation are addressed in this study.
31812816	1	4	from	bioreactor	255:264	arg1	performance					304:314	membrane performance	295:314	The impacts of salt accumulation, through adjusting the solid retention time (SRT), in the bioreactor on the bioprocess as well as membrane performance of a high retention nanofiltration membrane bioreactor (NF-MBR) and subsequent reverse osmosis (RO) process for water reclamation	164:444	The impacts of salt accumulation, through adjusting the solid retention time (SRT), in the bioreactor on the bioprocess as well as membrane performance of a high retention nanofiltration membrane bioreactor (NF-MBR) and subsequent reverse osmosis (RO) process for water reclamation are addressed in this study.
31812816	1	4	from	bioreactor	255:264	arg1	impacts					168:174	The impacts	164:174	The impacts of salt accumulation, through adjusting the solid retention time (SRT), in the bioreactor on the bioprocess as well as membrane performance of a high retention nanofiltration membrane bioreactor (NF-MBR) and subsequent reverse osmosis (RO) process for water reclamation	164:444	The impacts of salt accumulation, through adjusting the solid retention time (SRT), in the bioreactor on the bioprocess as well as membrane performance of a high retention nanofiltration membrane bioreactor (NF-MBR) and subsequent reverse osmosis (RO) process for water reclamation are addressed in this study.
31812816	1	5	theme	reverse	395:401	arg1	RO					412:413	RO	412:413	RO	412:413	The impacts of salt accumulation, through adjusting the solid retention time (SRT), in the bioreactor on the bioprocess as well as membrane performance of a high retention nanofiltration membrane bioreactor (NF-MBR) and subsequent reverse osmosis (RO) process for water reclamation are addressed in this study.
31812816	1	5	theme	reverse	395:401	arg1	osmosis					403:409	subsequent reverse osmosis	384:409	subsequent reverse osmosis (RO)	384:414	The impacts of salt accumulation, through adjusting the solid retention time (SRT), in the bioreactor on the bioprocess as well as membrane performance of a high retention nanofiltration membrane bioreactor (NF-MBR) and subsequent reverse osmosis (RO) process for water reclamation are addressed in this study.
31812816	6	6	theme	higher	1315:1320	arg1	rate					1342:1345	a higher RO membrane fouling rate	1313:1345	a higher RO membrane fouling rate	1313:1345	Although both NF-MBRs produced comparable quality of permeate, a higher RO membrane fouling rate was observed when the permeate of NF-MBR with SRT at 60 days was fed to the RO system, implying organic compositions in NF-MBR permeate may influence RO performance.
31812816	3	7	theme	>	793:793	arg1	%					797:797	> 98%	793:797	> 98%	793:797	Despite the accumulation of salts, both NF-MBRs at SRT of 30 and 60 days, achieved (i) similar biodegradation efficiency; (ii) excellent organic removal (> 97%); and (iii) excellent ammonia removal (> 98%).
31812816	3	7	theme	>	793:793	arg1	removal					784:790	(iii) excellent ammonia removal	760:790	(iii) excellent ammonia removal (> 98%)	760:798	Despite the accumulation of salts, both NF-MBRs at SRT of 30 and 60 days, achieved (i) similar biodegradation efficiency; (ii) excellent organic removal (> 97%); and (iii) excellent ammonia removal (> 98%).
31812816	5	8	theme	fouling	1174:1180	arg1	layer					1182:1186	irreversible fouling layer	1161:1186	irreversible fouling layer	1161:1186	However, more severe membrane fouling was observed in the NF-MBR with elevated salt levels, which was attributed to the greater formation of calcium phosphate scale and Ca-polysaccharides complex (i.e., irreversible fouling layer) as well as the cake-enhanced-osmotic-pressure (CEOP) effect.
31812816	3	9	theme	salts	622:626	arg1	accumulation					606:617	the accumulation	602:617	the accumulation of salts	602:626	Despite the accumulation of salts, both NF-MBRs at SRT of 30 and 60 days, achieved (i) similar biodegradation efficiency; (ii) excellent organic removal (> 97%); and (iii) excellent ammonia removal (> 98%).
31812816	6	10	from	60 days	1400:1406	arg1	NF-MBR					1381:1386	NF-MBR	1381:1386	NF-MBR with SRT at 60 days	1381:1406	Although both NF-MBRs produced comparable quality of permeate, a higher RO membrane fouling rate was observed when the permeate of NF-MBR with SRT at 60 days was fed to the RO system, implying organic compositions in NF-MBR permeate may influence RO performance.
31812816	1	11	theme	high	321:324	arg1	NF-MBR					372:377	NF-MBR	372:377	NF-MBR	372:377	The impacts of salt accumulation, through adjusting the solid retention time (SRT), in the bioreactor on the bioprocess as well as membrane performance of a high retention nanofiltration membrane bioreactor (NF-MBR) and subsequent reverse osmosis (RO) process for water reclamation are addressed in this study.
31812816	1	11	theme	high	321:324	arg1	bioreactor					360:369	a high retention nanofiltration membrane bioreactor	319:369	a high retention nanofiltration membrane bioreactor (NF-MBR)	319:378	The impacts of salt accumulation, through adjusting the solid retention time (SRT), in the bioreactor on the bioprocess as well as membrane performance of a high retention nanofiltration membrane bioreactor (NF-MBR) and subsequent reverse osmosis (RO) process for water reclamation are addressed in this study.
31812816	6	12	theme	comparable	1281:1290	arg1	quality					1292:1298	comparable quality	1281:1298	comparable quality of permeate	1281:1310	Although both NF-MBRs produced comparable quality of permeate, a higher RO membrane fouling rate was observed when the permeate of NF-MBR with SRT at 60 days was fed to the RO system, implying organic compositions in NF-MBR permeate may influence RO performance.
31812816	5	13	theme	severe	972:977	arg1	fouling					988:994	more severe membrane fouling	967:994	more severe membrane fouling	967:994	However, more severe membrane fouling was observed in the NF-MBR with elevated salt levels, which was attributed to the greater formation of calcium phosphate scale and Ca-polysaccharides complex (i.e., irreversible fouling layer) as well as the cake-enhanced-osmotic-pressure (CEOP) effect.
31812816	1	14	theme	nanofiltration	336:349	arg1	NF-MBR					372:377	NF-MBR	372:377	NF-MBR	372:377	The impacts of salt accumulation, through adjusting the solid retention time (SRT), in the bioreactor on the bioprocess as well as membrane performance of a high retention nanofiltration membrane bioreactor (NF-MBR) and subsequent reverse osmosis (RO) process for water reclamation are addressed in this study.
31812816	1	14	theme	nanofiltration	336:349	arg1	bioreactor					360:369	a high retention nanofiltration membrane bioreactor	319:369	a high retention nanofiltration membrane bioreactor (NF-MBR)	319:378	The impacts of salt accumulation, through adjusting the solid retention time (SRT), in the bioreactor on the bioprocess as well as membrane performance of a high retention nanofiltration membrane bioreactor (NF-MBR) and subsequent reverse osmosis (RO) process for water reclamation are addressed in this study.
31812816	1	15	theme	accumulation	184:195	arg1	performance					304:314	membrane performance	295:314	The impacts of salt accumulation, through adjusting the solid retention time (SRT), in the bioreactor on the bioprocess as well as membrane performance of a high retention nanofiltration membrane bioreactor (NF-MBR) and subsequent reverse osmosis (RO) process for water reclamation	164:444	The impacts of salt accumulation, through adjusting the solid retention time (SRT), in the bioreactor on the bioprocess as well as membrane performance of a high retention nanofiltration membrane bioreactor (NF-MBR) and subsequent reverse osmosis (RO) process for water reclamation are addressed in this study.
31812816	1	15	theme	accumulation	184:195	arg1	impacts					168:174	The impacts	164:174	The impacts of salt accumulation, through adjusting the solid retention time (SRT), in the bioreactor on the bioprocess as well as membrane performance of a high retention nanofiltration membrane bioreactor (NF-MBR) and subsequent reverse osmosis (RO) process for water reclamation	164:444	The impacts of salt accumulation, through adjusting the solid retention time (SRT), in the bioreactor on the bioprocess as well as membrane performance of a high retention nanofiltration membrane bioreactor (NF-MBR) and subsequent reverse osmosis (RO) process for water reclamation are addressed in this study.
31812816	1	16	theme	bioreactor	360:369	arg1	performance					304:314	membrane performance	295:314	The impacts of salt accumulation, through adjusting the solid retention time (SRT), in the bioreactor on the bioprocess as well as membrane performance of a high retention nanofiltration membrane bioreactor (NF-MBR) and subsequent reverse osmosis (RO) process for water reclamation	164:444	The impacts of salt accumulation, through adjusting the solid retention time (SRT), in the bioreactor on the bioprocess as well as membrane performance of a high retention nanofiltration membrane bioreactor (NF-MBR) and subsequent reverse osmosis (RO) process for water reclamation are addressed in this study.
31812816	1	16	theme	bioreactor	360:369	arg1	impacts					168:174	The impacts	164:174	The impacts of salt accumulation, through adjusting the solid retention time (SRT), in the bioreactor on the bioprocess as well as membrane performance of a high retention nanofiltration membrane bioreactor (NF-MBR) and subsequent reverse osmosis (RO) process for water reclamation	164:444	The impacts of salt accumulation, through adjusting the solid retention time (SRT), in the bioreactor on the bioprocess as well as membrane performance of a high retention nanofiltration membrane bioreactor (NF-MBR) and subsequent reverse osmosis (RO) process for water reclamation are addressed in this study.
31812816	1	17	from	impacts	168:174	arg1	bioreactor					255:264	the bioreactor	251:264	the bioreactor	251:264	The impacts of salt accumulation, through adjusting the solid retention time (SRT), in the bioreactor on the bioprocess as well as membrane performance of a high retention nanofiltration membrane bioreactor (NF-MBR) and subsequent reverse osmosis (RO) process for water reclamation are addressed in this study.
31812816	1	17	from	impacts	168:174	arg1	bioprocess					273:282	the bioprocess	269:282	the bioprocess	269:282	The impacts of salt accumulation, through adjusting the solid retention time (SRT), in the bioreactor on the bioprocess as well as membrane performance of a high retention nanofiltration membrane bioreactor (NF-MBR) and subsequent reverse osmosis (RO) process for water reclamation are addressed in this study.
31812816	5	18	with	effect	1242:1247	arg1	levels					1042:1047	elevated salt levels	1028:1047	elevated salt levels	1028:1047	However, more severe membrane fouling was observed in the NF-MBR with elevated salt levels, which was attributed to the greater formation of calcium phosphate scale and Ca-polysaccharides complex (i.e., irreversible fouling layer) as well as the cake-enhanced-osmotic-pressure (CEOP) effect.
31812816	3	19	theme	biodegradation	689:702	arg1	efficiency					704:713	similar biodegradation efficiency	681:713	similar biodegradation efficiency	681:713	Despite the accumulation of salts, both NF-MBRs at SRT of 30 and 60 days, achieved (i) similar biodegradation efficiency; (ii) excellent organic removal (> 97%); and (iii) excellent ammonia removal (> 98%).
31812816	2	20	theme	hydraulic	539:547	arg1	HRT					565:567	HRT	565:567	HRT	565:567	The build-up of salts (i.e., Ca, Mg, PO4) is a function of SRT, hydraulic retention time (HRT) and membrane rejection.
31812816	2	20	theme	hydraulic	539:547	arg1	time					559:562	hydraulic retention time	539:562	hydraulic retention time (HRT)	539:568	The build-up of salts (i.e., Ca, Mg, PO4) is a function of SRT, hydraulic retention time (HRT) and membrane rejection.
31812816	2	20	theme	hydraulic	539:547	arg1	function					522:529	a function	520:529	a function of SRT	520:536	The build-up of salts (i.e., Ca, Mg, PO4) is a function of SRT, hydraulic retention time (HRT) and membrane rejection.
31812816	1	21	from	performance	304:314	arg1	bioreactor					255:264	the bioreactor	251:264	the bioreactor	251:264	The impacts of salt accumulation, through adjusting the solid retention time (SRT), in the bioreactor on the bioprocess as well as membrane performance of a high retention nanofiltration membrane bioreactor (NF-MBR) and subsequent reverse osmosis (RO) process for water reclamation are addressed in this study.
31812816	1	21	from	performance	304:314	arg1	bioprocess					273:282	the bioprocess	269:282	the bioprocess	269:282	The impacts of salt accumulation, through adjusting the solid retention time (SRT), in the bioreactor on the bioprocess as well as membrane performance of a high retention nanofiltration membrane bioreactor (NF-MBR) and subsequent reverse osmosis (RO) process for water reclamation are addressed in this study.
31812816	6	22	theme	membrane	1325:1332	arg1	rate					1342:1345	a higher RO membrane fouling rate	1313:1345	a higher RO membrane fouling rate	1313:1345	Although both NF-MBRs produced comparable quality of permeate, a higher RO membrane fouling rate was observed when the permeate of NF-MBR with SRT at 60 days was fed to the RO system, implying organic compositions in NF-MBR permeate may influence RO performance.
31812816	6	23	dep	NF-MBR	1467:1472	arg1	permeate					1474:1481	permeate	1474:1481	permeate	1474:1481	Although both NF-MBRs produced comparable quality of permeate, a higher RO membrane fouling rate was observed when the permeate of NF-MBR with SRT at 60 days was fed to the RO system, implying organic compositions in NF-MBR permeate may influence RO performance.
31812816	6	24	theme	RO	1497:1498	arg1	performance					1500:1510	RO performance	1497:1510	RO performance	1497:1510	Although both NF-MBRs produced comparable quality of permeate, a higher RO membrane fouling rate was observed when the permeate of NF-MBR with SRT at 60 days was fed to the RO system, implying organic compositions in NF-MBR permeate may influence RO performance.
31812816	5	25	with	NF-MBR	1016:1021	arg1	levels					1042:1047	elevated salt levels	1028:1047	elevated salt levels	1028:1047	However, more severe membrane fouling was observed in the NF-MBR with elevated salt levels, which was attributed to the greater formation of calcium phosphate scale and Ca-polysaccharides complex (i.e., irreversible fouling layer) as well as the cake-enhanced-osmotic-pressure (CEOP) effect.
31812816	6	26	dep	the	1365:1367	arg1	permeate					1369:1376	permeate	1369:1376	permeate	1369:1376	Although both NF-MBRs produced comparable quality of permeate, a higher RO membrane fouling rate was observed when the permeate of NF-MBR with SRT at 60 days was fed to the RO system, implying organic compositions in NF-MBR permeate may influence RO performance.
31812816	0	27	from	Impact	0:5	arg1	bioreactor					35:44	the bioreactor	31:44	the bioreactor	31:44	Impact of salt accumulation in the bioreactor on the performance of nanofiltration membrane bioreactor (NF-MBR)+Reverse osmosis (RO) process for water reclamation.
31812816	0	27	from	Impact	0:5	arg1	performance					53:63	the performance	49:63	the performance of nanofiltration membrane bioreactor (NF-MBR)	49:110	Impact of salt accumulation in the bioreactor on the performance of nanofiltration membrane bioreactor (NF-MBR)+Reverse osmosis (RO) process for water reclamation.
31812816	2	28	theme	SRT	534:536	arg1	build-up					479:486	The build-up	475:486	The build-up of salts (i.e., Ca, Mg, PO4)	475:515	The build-up of salts (i.e., Ca, Mg, PO4) is a function of SRT, hydraulic retention time (HRT) and membrane rejection.
31812816	2	28	theme	SRT	534:536	arg1	function					522:529	a function	520:529	a function of SRT	520:536	The build-up of salts (i.e., Ca, Mg, PO4) is a function of SRT, hydraulic retention time (HRT) and membrane rejection.
31812816	2	28	theme	SRT	534:536	arg1	time					559:562	hydraulic retention time	539:562	hydraulic retention time (HRT)	539:568	The build-up of salts (i.e., Ca, Mg, PO4) is a function of SRT, hydraulic retention time (HRT) and membrane rejection.
31812816	2	28	theme	SRT	534:536	arg1	rejection					583:591	membrane rejection	574:591	membrane rejection	574:591	The build-up of salts (i.e., Ca, Mg, PO4) is a function of SRT, hydraulic retention time (HRT) and membrane rejection.
31812816	5	29	theme	salt	1037:1040	arg1	levels					1042:1047	elevated salt levels	1028:1047	elevated salt levels	1028:1047	However, more severe membrane fouling was observed in the NF-MBR with elevated salt levels, which was attributed to the greater formation of calcium phosphate scale and Ca-polysaccharides complex (i.e., irreversible fouling layer) as well as the cake-enhanced-osmotic-pressure (CEOP) effect.
31812816	0	30	theme	membrane	83:90	arg1	bioreactor					92:101	nanofiltration membrane bioreactor	68:101	nanofiltration membrane bioreactor (NF-MBR)	68:110	Impact of salt accumulation in the bioreactor on the performance of nanofiltration membrane bioreactor (NF-MBR)+Reverse osmosis (RO) process for water reclamation.
31812816	0	30	theme	membrane	83:90	arg1	NF-MBR					104:109	NF-MBR	104:109	NF-MBR	104:109	Impact of salt accumulation in the bioreactor on the performance of nanofiltration membrane bioreactor (NF-MBR)+Reverse osmosis (RO) process for water reclamation.
31812816	1	31	theme	membrane	295:302	arg1	performance					304:314	membrane performance	295:314	The impacts of salt accumulation, through adjusting the solid retention time (SRT), in the bioreactor on the bioprocess as well as membrane performance of a high retention nanofiltration membrane bioreactor (NF-MBR) and subsequent reverse osmosis (RO) process for water reclamation	164:444	The impacts of salt accumulation, through adjusting the solid retention time (SRT), in the bioreactor on the bioprocess as well as membrane performance of a high retention nanofiltration membrane bioreactor (NF-MBR) and subsequent reverse osmosis (RO) process for water reclamation are addressed in this study.
31812816	0	32	from	bioreactor	35:44	arg1	Impact					0:5	Impact	0:5	Impact of salt accumulation in the bioreactor on the performance of nanofiltration membrane bioreactor (NF-MBR)	0:110	Impact of salt accumulation in the bioreactor on the performance of nanofiltration membrane bioreactor (NF-MBR)+Reverse osmosis (RO) process for water reclamation.
31812816	3	33	theme	excellent	721:729	arg1	removal					739:745	(ii) excellent organic removal	716:745	(ii) excellent organic removal (> 97%)	716:753	Despite the accumulation of salts, both NF-MBRs at SRT of 30 and 60 days, achieved (i) similar biodegradation efficiency; (ii) excellent organic removal (> 97%); and (iii) excellent ammonia removal (> 98%).
31812816	3	33	theme	excellent	721:729	arg1	%					752:752	> 97%	748:752	> 97%	748:752	Despite the accumulation of salts, both NF-MBRs at SRT of 30 and 60 days, achieved (i) similar biodegradation efficiency; (ii) excellent organic removal (> 97%); and (iii) excellent ammonia removal (> 98%).
31812816	5	34	theme	phosphate	1107:1115	arg1	formation					1086:1094	the greater formation	1074:1094	the greater formation of calcium phosphate scale and Ca-polysaccharides complex (i.e., irreversible fouling layer)	1074:1187	However, more severe membrane fouling was observed in the NF-MBR with elevated salt levels, which was attributed to the greater formation of calcium phosphate scale and Ca-polysaccharides complex (i.e., irreversible fouling layer) as well as the cake-enhanced-osmotic-pressure (CEOP) effect.
31812816	5	35	theme	cake-enhanced-osmotic-pressure	1204:1233	arg1	effect					1242:1247	the cake-enhanced-osmotic-pressure (CEOP) effect	1200:1247	the cake-enhanced-osmotic-pressure (CEOP) effect	1200:1247	However, more severe membrane fouling was observed in the NF-MBR with elevated salt levels, which was attributed to the greater formation of calcium phosphate scale and Ca-polysaccharides complex (i.e., irreversible fouling layer) as well as the cake-enhanced-osmotic-pressure (CEOP) effect.
31812816	0	36	theme	salt	10:13	arg1	accumulation					15:26	salt accumulation	10:26	salt accumulation in the bioreactor	10:44	Impact of salt accumulation in the bioreactor on the performance of nanofiltration membrane bioreactor (NF-MBR)+Reverse osmosis (RO) process for water reclamation.
31812816	3	37	theme	>	748:748	arg1	removal					739:745	(ii) excellent organic removal	716:745	(ii) excellent organic removal (> 97%)	716:753	Despite the accumulation of salts, both NF-MBRs at SRT of 30 and 60 days, achieved (i) similar biodegradation efficiency; (ii) excellent organic removal (> 97%); and (iii) excellent ammonia removal (> 98%).
31812816	3	37	theme	>	748:748	arg1	%					752:752	> 97%	748:752	> 97%	748:752	Despite the accumulation of salts, both NF-MBRs at SRT of 30 and 60 days, achieved (i) similar biodegradation efficiency; (ii) excellent organic removal (> 97%); and (iii) excellent ammonia removal (> 98%).
31812816	5	38	theme	greater	1078:1084	arg1	formation					1086:1094	the greater formation	1074:1094	the greater formation of calcium phosphate scale and Ca-polysaccharides complex (i.e., irreversible fouling layer)	1074:1187	However, more severe membrane fouling was observed in the NF-MBR with elevated salt levels, which was attributed to the greater formation of calcium phosphate scale and Ca-polysaccharides complex (i.e., irreversible fouling layer) as well as the cake-enhanced-osmotic-pressure (CEOP) effect.
31812816	1	39	theme	water	428:432	arg1	reclamation					434:444	water reclamation	428:444	water reclamation	428:444	The impacts of salt accumulation, through adjusting the solid retention time (SRT), in the bioreactor on the bioprocess as well as membrane performance of a high retention nanofiltration membrane bioreactor (NF-MBR) and subsequent reverse osmosis (RO) process for water reclamation are addressed in this study.
31812816	6	40	with	NF-MBR	1381:1386	arg1	SRT					1393:1395	SRT	1393:1395	SRT	1393:1395	Although both NF-MBRs produced comparable quality of permeate, a higher RO membrane fouling rate was observed when the permeate of NF-MBR with SRT at 60 days was fed to the RO system, implying organic compositions in NF-MBR permeate may influence RO performance.
31812816	4	41	theme	bio-flocculation	847:862	arg1	capability					864:873	the microbial bio-flocculation capability	833:873	the microbial bio-flocculation capability	833:873	Extending the SRT could improve the microbial bio-flocculation capability, but did not influence the microbial activity, viability, and community structure.
31812816	5	42	theme	irreversible	1161:1172	arg1	layer					1182:1186	irreversible fouling layer	1161:1186	irreversible fouling layer	1161:1186	However, more severe membrane fouling was observed in the NF-MBR with elevated salt levels, which was attributed to the greater formation of calcium phosphate scale and Ca-polysaccharides complex (i.e., irreversible fouling layer) as well as the cake-enhanced-osmotic-pressure (CEOP) effect.
31812816	3	43	theme	ammonia	776:782	arg1	%					797:797	> 98%	793:797	> 98%	793:797	Despite the accumulation of salts, both NF-MBRs at SRT of 30 and 60 days, achieved (i) similar biodegradation efficiency; (ii) excellent organic removal (> 97%); and (iii) excellent ammonia removal (> 98%).
31812816	3	43	theme	ammonia	776:782	arg1	removal					784:790	(iii) excellent ammonia removal	760:790	(iii) excellent ammonia removal (> 98%)	760:798	Despite the accumulation of salts, both NF-MBRs at SRT of 30 and 60 days, achieved (i) similar biodegradation efficiency; (ii) excellent organic removal (> 97%); and (iii) excellent ammonia removal (> 98%).
31812816	2	44	dep	salts	491:495	arg1	Mg					508:509	Mg	508:509	Mg	508:509	The build-up of salts (i.e., Ca, Mg, PO4) is a function of SRT, hydraulic retention time (HRT) and membrane rejection.
31812816	2	44	dep	salts	491:495	arg1	Ca					504:505	Ca	504:505	Ca	504:505	The build-up of salts (i.e., Ca, Mg, PO4) is a function of SRT, hydraulic retention time (HRT) and membrane rejection.
31812816	5	45	theme	complex	1146:1152	arg1	formation					1086:1094	the greater formation	1074:1094	the greater formation of calcium phosphate scale and Ca-polysaccharides complex (i.e., irreversible fouling layer)	1074:1187	However, more severe membrane fouling was observed in the NF-MBR with elevated salt levels, which was attributed to the greater formation of calcium phosphate scale and Ca-polysaccharides complex (i.e., irreversible fouling layer) as well as the cake-enhanced-osmotic-pressure (CEOP) effect.
31812816	1	46	theme	solid	220:224	arg1	SRT					242:244	SRT	242:244	SRT	242:244	The impacts of salt accumulation, through adjusting the solid retention time (SRT), in the bioreactor on the bioprocess as well as membrane performance of a high retention nanofiltration membrane bioreactor (NF-MBR) and subsequent reverse osmosis (RO) process for water reclamation are addressed in this study.
31812816	1	46	theme	solid	220:224	arg1	time					236:239	the solid retention time	216:239	the solid retention time (SRT)	216:245	The impacts of salt accumulation, through adjusting the solid retention time (SRT), in the bioreactor on the bioprocess as well as membrane performance of a high retention nanofiltration membrane bioreactor (NF-MBR) and subsequent reverse osmosis (RO) process for water reclamation are addressed in this study.
31812816	1	47	theme	subsequent	384:393	arg1	RO					412:413	RO	412:413	RO	412:413	The impacts of salt accumulation, through adjusting the solid retention time (SRT), in the bioreactor on the bioprocess as well as membrane performance of a high retention nanofiltration membrane bioreactor (NF-MBR) and subsequent reverse osmosis (RO) process for water reclamation are addressed in this study.
31812816	1	47	theme	subsequent	384:393	arg1	osmosis					403:409	subsequent reverse osmosis	384:409	subsequent reverse osmosis (RO)	384:414	The impacts of salt accumulation, through adjusting the solid retention time (SRT), in the bioreactor on the bioprocess as well as membrane performance of a high retention nanofiltration membrane bioreactor (NF-MBR) and subsequent reverse osmosis (RO) process for water reclamation are addressed in this study.
31812816	5	48	theme	membrane	979:986	arg1	fouling					988:994	more severe membrane fouling	967:994	more severe membrane fouling	967:994	However, more severe membrane fouling was observed in the NF-MBR with elevated salt levels, which was attributed to the greater formation of calcium phosphate scale and Ca-polysaccharides complex (i.e., irreversible fouling layer) as well as the cake-enhanced-osmotic-pressure (CEOP) effect.
31812816	4	49	theme	microbial	902:910	arg1	activity					912:919	the microbial activity	898:919	the microbial activity	898:919	Extending the SRT could improve the microbial bio-flocculation capability, but did not influence the microbial activity, viability, and community structure.
31812816	1	50	theme	osmosis	403:409	arg1	performance					304:314	membrane performance	295:314	The impacts of salt accumulation, through adjusting the solid retention time (SRT), in the bioreactor on the bioprocess as well as membrane performance of a high retention nanofiltration membrane bioreactor (NF-MBR) and subsequent reverse osmosis (RO) process for water reclamation	164:444	The impacts of salt accumulation, through adjusting the solid retention time (SRT), in the bioreactor on the bioprocess as well as membrane performance of a high retention nanofiltration membrane bioreactor (NF-MBR) and subsequent reverse osmosis (RO) process for water reclamation are addressed in this study.
31812816	1	50	theme	osmosis	403:409	arg1	impacts					168:174	The impacts	164:174	The impacts of salt accumulation, through adjusting the solid retention time (SRT), in the bioreactor on the bioprocess as well as membrane performance of a high retention nanofiltration membrane bioreactor (NF-MBR) and subsequent reverse osmosis (RO) process for water reclamation	164:444	The impacts of salt accumulation, through adjusting the solid retention time (SRT), in the bioreactor on the bioprocess as well as membrane performance of a high retention nanofiltration membrane bioreactor (NF-MBR) and subsequent reverse osmosis (RO) process for water reclamation are addressed in this study.
31812816	6	51	theme	permeate	1303:1310	arg1	quality					1292:1298	comparable quality	1281:1298	comparable quality of permeate	1281:1310	Although both NF-MBRs produced comparable quality of permeate, a higher RO membrane fouling rate was observed when the permeate of NF-MBR with SRT at 60 days was fed to the RO system, implying organic compositions in NF-MBR permeate may influence RO performance.
31812816	2	52	theme	PO4	512:514	arg1	Mg					508:509	Mg	508:509	Mg	508:509	The build-up of salts (i.e., Ca, Mg, PO4) is a function of SRT, hydraulic retention time (HRT) and membrane rejection.
31812816	2	52	theme	PO4	512:514	arg1	Ca					504:505	Ca	504:505	Ca	504:505	The build-up of salts (i.e., Ca, Mg, PO4) is a function of SRT, hydraulic retention time (HRT) and membrane rejection.
31812816	6	53	theme	organic	1443:1449	arg1	compositions					1451:1462	organic compositions	1443:1462	organic compositions in NF-MBR permeate	1443:1481	Although both NF-MBRs produced comparable quality of permeate, a higher RO membrane fouling rate was observed when the permeate of NF-MBR with SRT at 60 days was fed to the RO system, implying organic compositions in NF-MBR permeate may influence RO performance.
31812816	1	54	theme	retention	326:334	arg1	NF-MBR					372:377	NF-MBR	372:377	NF-MBR	372:377	The impacts of salt accumulation, through adjusting the solid retention time (SRT), in the bioreactor on the bioprocess as well as membrane performance of a high retention nanofiltration membrane bioreactor (NF-MBR) and subsequent reverse osmosis (RO) process for water reclamation are addressed in this study.
31812816	1	54	theme	retention	326:334	arg1	bioreactor					360:369	a high retention nanofiltration membrane bioreactor	319:369	a high retention nanofiltration membrane bioreactor (NF-MBR)	319:378	The impacts of salt accumulation, through adjusting the solid retention time (SRT), in the bioreactor on the bioprocess as well as membrane performance of a high retention nanofiltration membrane bioreactor (NF-MBR) and subsequent reverse osmosis (RO) process for water reclamation are addressed in this study.
31812816	3	55	dep	removal	784:790	arg1	iii					761:763	iii	761:763	iii	761:763	Despite the accumulation of salts, both NF-MBRs at SRT of 30 and 60 days, achieved (i) similar biodegradation efficiency; (ii) excellent organic removal (> 97%); and (iii) excellent ammonia removal (> 98%).
31812816	1	56	theme	salt	179:182	arg1	accumulation					184:195	salt accumulation	179:195	salt accumulation, through adjusting the solid retention time (SRT), in the bioreactor	179:264	The impacts of salt accumulation, through adjusting the solid retention time (SRT), in the bioreactor on the bioprocess as well as membrane performance of a high retention nanofiltration membrane bioreactor (NF-MBR) and subsequent reverse osmosis (RO) process for water reclamation are addressed in this study.
31812816	2	57	dep	Ca	504:505	arg1	i.e.					498:501	i.e.	498:501	i.e.	498:501	The build-up of salts (i.e., Ca, Mg, PO4) is a function of SRT, hydraulic retention time (HRT) and membrane rejection.
31812816	2	58	theme	membrane	574:581	arg1	function					522:529	a function	520:529	a function of SRT	520:536	The build-up of salts (i.e., Ca, Mg, PO4) is a function of SRT, hydraulic retention time (HRT) and membrane rejection.
31812816	2	58	theme	membrane	574:581	arg1	rejection					583:591	membrane rejection	574:591	membrane rejection	574:591	The build-up of salts (i.e., Ca, Mg, PO4) is a function of SRT, hydraulic retention time (HRT) and membrane rejection.
31812816	1	59	theme	membrane	351:358	arg1	NF-MBR					372:377	NF-MBR	372:377	NF-MBR	372:377	The impacts of salt accumulation, through adjusting the solid retention time (SRT), in the bioreactor on the bioprocess as well as membrane performance of a high retention nanofiltration membrane bioreactor (NF-MBR) and subsequent reverse osmosis (RO) process for water reclamation are addressed in this study.
31812816	1	59	theme	membrane	351:358	arg1	bioreactor					360:369	a high retention nanofiltration membrane bioreactor	319:369	a high retention nanofiltration membrane bioreactor (NF-MBR)	319:378	The impacts of salt accumulation, through adjusting the solid retention time (SRT), in the bioreactor on the bioprocess as well as membrane performance of a high retention nanofiltration membrane bioreactor (NF-MBR) and subsequent reverse osmosis (RO) process for water reclamation are addressed in this study.
31812816	1	60	from	accumulation	184:195	arg1	bioreactor					255:264	the bioreactor	251:264	the bioreactor	251:264	The impacts of salt accumulation, through adjusting the solid retention time (SRT), in the bioreactor on the bioprocess as well as membrane performance of a high retention nanofiltration membrane bioreactor (NF-MBR) and subsequent reverse osmosis (RO) process for water reclamation are addressed in this study.
31812816	6	61	theme	RO	1423:1424	arg1	system					1426:1431	the RO system	1419:1431	the RO system	1419:1431	Although both NF-MBRs produced comparable quality of permeate, a higher RO membrane fouling rate was observed when the permeate of NF-MBR with SRT at 60 days was fed to the RO system, implying organic compositions in NF-MBR permeate may influence RO performance.
31812816	2	62	theme	salts	491:495	arg1	build-up					479:486	The build-up	475:486	The build-up of salts (i.e., Ca, Mg, PO4)	475:515	The build-up of salts (i.e., Ca, Mg, PO4) is a function of SRT, hydraulic retention time (HRT) and membrane rejection.
31812816	2	62	theme	salts	491:495	arg1	function					522:529	a function	520:529	a function of SRT	520:536	The build-up of salts (i.e., Ca, Mg, PO4) is a function of SRT, hydraulic retention time (HRT) and membrane rejection.
31812816	3	63	theme	60 days	659:665	arg1	SRT					645:647	SRT	645:647	SRT of 30 and 60 days	645:665	Despite the accumulation of salts, both NF-MBRs at SRT of 30 and 60 days, achieved (i) similar biodegradation efficiency; (ii) excellent organic removal (> 97%); and (iii) excellent ammonia removal (> 98%).
31812816	6	64	theme	fouling	1334:1340	arg1	rate					1342:1345	a higher RO membrane fouling rate	1313:1345	a higher RO membrane fouling rate	1313:1345	Although both NF-MBRs produced comparable quality of permeate, a higher RO membrane fouling rate was observed when the permeate of NF-MBR with SRT at 60 days was fed to the RO system, implying organic compositions in NF-MBR permeate may influence RO performance.
31812816	3	65	theme	similar	681:687	arg1	efficiency					704:713	similar biodegradation efficiency	681:713	similar biodegradation efficiency	681:713	Despite the accumulation of salts, both NF-MBRs at SRT of 30 and 60 days, achieved (i) similar biodegradation efficiency; (ii) excellent organic removal (> 97%); and (iii) excellent ammonia removal (> 98%).
31812816	0	66	from	accumulation	15:26	arg1	bioreactor					35:44	the bioreactor	31:44	the bioreactor	31:44	Impact of salt accumulation in the bioreactor on the performance of nanofiltration membrane bioreactor (NF-MBR)+Reverse osmosis (RO) process for water reclamation.
31812816	4	67	theme	community	937:945	arg1	structure					947:955	community structure	937:955	community structure	937:955	Extending the SRT could improve the microbial bio-flocculation capability, but did not influence the microbial activity, viability, and community structure.
31812816	3	68	theme	30	652:653	arg1	SRT					645:647	SRT	645:647	SRT of 30 and 60 days	645:665	Despite the accumulation of salts, both NF-MBRs at SRT of 30 and 60 days, achieved (i) similar biodegradation efficiency; (ii) excellent organic removal (> 97%); and (iii) excellent ammonia removal (> 98%).
31812816	0	69	theme	nanofiltration	68:81	arg1	bioreactor					92:101	nanofiltration membrane bioreactor	68:101	nanofiltration membrane bioreactor (NF-MBR)	68:110	Impact of salt accumulation in the bioreactor on the performance of nanofiltration membrane bioreactor (NF-MBR)+Reverse osmosis (RO) process for water reclamation.
31812816	0	69	theme	nanofiltration	68:81	arg1	NF-MBR					104:109	NF-MBR	104:109	NF-MBR	104:109	Impact of salt accumulation in the bioreactor on the performance of nanofiltration membrane bioreactor (NF-MBR)+Reverse osmosis (RO) process for water reclamation.
31812816	6	70	theme	RO	1322:1323	arg1	rate					1342:1345	a higher RO membrane fouling rate	1313:1345	a higher RO membrane fouling rate	1313:1345	Although both NF-MBRs produced comparable quality of permeate, a higher RO membrane fouling rate was observed when the permeate of NF-MBR with SRT at 60 days was fed to the RO system, implying organic compositions in NF-MBR permeate may influence RO performance.
31812816	5	71	theme	elevated	1028:1035	arg1	levels					1042:1047	elevated salt levels	1028:1047	elevated salt levels	1028:1047	However, more severe membrane fouling was observed in the NF-MBR with elevated salt levels, which was attributed to the greater formation of calcium phosphate scale and Ca-polysaccharides complex (i.e., irreversible fouling layer) as well as the cake-enhanced-osmotic-pressure (CEOP) effect.
31812816	0	72	theme	bioreactor	92:101	arg1	performance					53:63	the performance	49:63	the performance of nanofiltration membrane bioreactor (NF-MBR)	49:110	Impact of salt accumulation in the bioreactor on the performance of nanofiltration membrane bioreactor (NF-MBR)+Reverse osmosis (RO) process for water reclamation.
31812816	5	73	dep	layer	1182:1186	arg1	i.e.					1155:1158	i.e.	1155:1158	i.e.	1155:1158	However, more severe membrane fouling was observed in the NF-MBR with elevated salt levels, which was attributed to the greater formation of calcium phosphate scale and Ca-polysaccharides complex (i.e., irreversible fouling layer) as well as the cake-enhanced-osmotic-pressure (CEOP) effect.
31812816	6	74	from	compositions	1451:1462	arg1	NF-MBR					1467:1472	NF-MBR	1467:1472	NF-MBR permeate	1467:1481	Although both NF-MBRs produced comparable quality of permeate, a higher RO membrane fouling rate was observed when the permeate of NF-MBR with SRT at 60 days was fed to the RO system, implying organic compositions in NF-MBR permeate may influence RO performance.
31812816	3	75	dep	efficiency	704:713	arg1	i					678:678	i	678:678	i	678:678	Despite the accumulation of salts, both NF-MBRs at SRT of 30 and 60 days, achieved (i) similar biodegradation efficiency; (ii) excellent organic removal (> 97%); and (iii) excellent ammonia removal (> 98%).
31812816	5	76	theme	CEOP	1236:1239	arg1	effect					1242:1247	the cake-enhanced-osmotic-pressure (CEOP) effect	1200:1247	the cake-enhanced-osmotic-pressure (CEOP) effect	1200:1247	However, more severe membrane fouling was observed in the NF-MBR with elevated salt levels, which was attributed to the greater formation of calcium phosphate scale and Ca-polysaccharides complex (i.e., irreversible fouling layer) as well as the cake-enhanced-osmotic-pressure (CEOP) effect.
31812816	5	77	theme	calcium	1099:1105	arg1	phosphate					1107:1115	calcium phosphate scale	1099:1121	calcium phosphate scale	1099:1121	However, more severe membrane fouling was observed in the NF-MBR with elevated salt levels, which was attributed to the greater formation of calcium phosphate scale and Ca-polysaccharides complex (i.e., irreversible fouling layer) as well as the cake-enhanced-osmotic-pressure (CEOP) effect.
31812816	3	78	theme	organic	731:737	arg1	removal					739:745	(ii) excellent organic removal	716:745	(ii) excellent organic removal (> 97%)	716:753	Despite the accumulation of salts, both NF-MBRs at SRT of 30 and 60 days, achieved (i) similar biodegradation efficiency; (ii) excellent organic removal (> 97%); and (iii) excellent ammonia removal (> 98%).
31812816	3	78	theme	organic	731:737	arg1	%					752:752	> 97%	748:752	> 97%	748:752	Despite the accumulation of salts, both NF-MBRs at SRT of 30 and 60 days, achieved (i) similar biodegradation efficiency; (ii) excellent organic removal (> 97%); and (iii) excellent ammonia removal (> 98%).
31812816	2	79	theme	retention	549:557	arg1	HRT					565:567	HRT	565:567	HRT	565:567	The build-up of salts (i.e., Ca, Mg, PO4) is a function of SRT, hydraulic retention time (HRT) and membrane rejection.
31812816	2	79	theme	retention	549:557	arg1	time					559:562	hydraulic retention time	539:562	hydraulic retention time (HRT)	539:568	The build-up of salts (i.e., Ca, Mg, PO4) is a function of SRT, hydraulic retention time (HRT) and membrane rejection.
31812816	2	79	theme	retention	549:557	arg1	function					522:529	a function	520:529	a function of SRT	520:536	The build-up of salts (i.e., Ca, Mg, PO4) is a function of SRT, hydraulic retention time (HRT) and membrane rejection.
31812816	0	80	theme	accumulation	15:26	arg1	Impact					0:5	Impact	0:5	Impact of salt accumulation in the bioreactor on the performance of nanofiltration membrane bioreactor (NF-MBR)	0:110	Impact of salt accumulation in the bioreactor on the performance of nanofiltration membrane bioreactor (NF-MBR)+Reverse osmosis (RO) process for water reclamation.
31812816	5	81	located	observed	1000:1007	arg1	NF-MBR					1016:1021	the NF-MBR	1012:1021	the NF-MBR with elevated salt levels, which was attributed to the greater formation of calcium phosphate scale and Ca-polysaccharides complex (i.e., irreversible fouling layer)	1012:1187	However, more severe membrane fouling was observed in the NF-MBR with elevated salt levels, which was attributed to the greater formation of calcium phosphate scale and Ca-polysaccharides complex (i.e., irreversible fouling layer) as well as the cake-enhanced-osmotic-pressure (CEOP) effect.
31812816	5	81	located	observed	1000:1007	arg1	effect					1242:1247	the cake-enhanced-osmotic-pressure (CEOP) effect	1200:1247	the cake-enhanced-osmotic-pressure (CEOP) effect	1200:1247	However, more severe membrane fouling was observed in the NF-MBR with elevated salt levels, which was attributed to the greater formation of calcium phosphate scale and Ca-polysaccharides complex (i.e., irreversible fouling layer) as well as the cake-enhanced-osmotic-pressure (CEOP) effect.
31812816	5	81	located	observed	1000:1007	arg2	fouling					988:994	more severe membrane fouling	967:994	more severe membrane fouling	967:994	However, more severe membrane fouling was observed in the NF-MBR with elevated salt levels, which was attributed to the greater formation of calcium phosphate scale and Ca-polysaccharides complex (i.e., irreversible fouling layer) as well as the cake-enhanced-osmotic-pressure (CEOP) effect.
31812816	0	82	theme	osmosis	120:126	arg1	process					133:139	osmosis (RO) process	120:139	osmosis (RO) process for water reclamation	120:161	Impact of salt accumulation in the bioreactor on the performance of nanofiltration membrane bioreactor (NF-MBR)+Reverse osmosis (RO) process for water reclamation.
31812816	4	83	theme	microbial	837:845	arg1	capability					864:873	the microbial bio-flocculation capability	833:873	the microbial bio-flocculation capability	833:873	Extending the SRT could improve the microbial bio-flocculation capability, but did not influence the microbial activity, viability, and community structure.
31812816	1	84	dep	accumulation	184:195	arg1	adjusting					206:214	adjusting	206:214	adjusting the solid retention time (SRT)	206:245	The impacts of salt accumulation, through adjusting the solid retention time (SRT), in the bioreactor on the bioprocess as well as membrane performance of a high retention nanofiltration membrane bioreactor (NF-MBR) and subsequent reverse osmosis (RO) process for water reclamation are addressed in this study.
31812816	5	85	theme	scale	1117:1121	arg1	phosphate					1107:1115	calcium phosphate scale	1099:1121	calcium phosphate scale	1099:1121	However, more severe membrane fouling was observed in the NF-MBR with elevated salt levels, which was attributed to the greater formation of calcium phosphate scale and Ca-polysaccharides complex (i.e., irreversible fouling layer) as well as the cake-enhanced-osmotic-pressure (CEOP) effect.
31812816	5	86	theme	Ca-polysaccharides	1127:1144	arg1	complex					1146:1152	Ca-polysaccharides complex	1127:1152	Ca-polysaccharides complex	1127:1152	However, more severe membrane fouling was observed in the NF-MBR with elevated salt levels, which was attributed to the greater formation of calcium phosphate scale and Ca-polysaccharides complex (i.e., irreversible fouling layer) as well as the cake-enhanced-osmotic-pressure (CEOP) effect.
30836102	11	0	theme	Oligosaccharide	1658:1672	arg1	deletions					1679:1687	Oligosaccharide site deletions	1658:1687	Oligosaccharide site deletions at positions Asn56 and Asn79 on the α-subunit and Asn5 on the β-subunit	1658:1759	Oligosaccharide site deletions at positions Asn56 and Asn79 on the α-subunit and Asn5 on the β-subunit were found to play an essential role in cAMP signal transduction through the eelFSH receptor.
30836102	7	1	theme	molecular	1140:1148	arg1	weight					1150:1155	The molecular weight	1136:1155	The molecular weight of rec-eelFSHβ/α wild-type by western blotting analysis	1136:1211	The molecular weight of rec-eelFSHβ/α wild-type by western blotting analysis was found to be 34 kDa.
30836102	7	1	theme	molecular	1140:1148	arg1	34 kDa					1229:1234	34 kDa	1229:1234	34 kDa	1229:1234	The molecular weight of rec-eelFSHβ/α wild-type by western blotting analysis was found to be 34 kDa.
30836102	5	2	from	lysate	986:991	arg1	medium					970:975	the medium	966:975	the medium	966:975	The absence of the Asn22 (eelFSHβΔ22/α) and Asn5.22 (eelFSHβΔ5.22/α) N-linked oligosaccharide chain in the eelFSH β-subunit completely reduced the secretion level in the medium and cell lysate of CHO-K1 cells.
30836102	1	3	theme	N-linked	272:279	arg1	residues					294:301	two N-linked carbohydrate residues	268:301	two N-linked carbohydrate residues	268:301	Eel follicle-stimulating hormone (eelFSH) is composed of a common α-subunit and a hormone specific β-subunit, both of which contain two N-linked carbohydrate residues.
30836102	2	4	theme	terminal	399:406	arg1	region					408:413	the carboxyl terminal region	386:413	the carboxyl terminal region of the eelFSH β-subunit	386:437	We characterized the biologically active single chains by fusing the α-subunit to the carboxyl terminal region of the eelFSH β-subunit.
30836102	4	5	theme	double	679:684	arg1	sites					699:703	the individual and double glycosylated sites	660:703	the individual and double glycosylated sites	660:703	Mutagenesis of the individual and double glycosylated sites was performed to determine the functions of the oligosaccharide chains on signal transduction.
30836102	5	6	theme	chain	894:898	arg1	absence					804:810	The absence	800:810	The absence of the Asn22 (eelFSHβΔ22/α) and Asn5.22 (eelFSHβΔ5.22/α) N-linked oligosaccharide chain in the eelFSH β-subunit	800:922	The absence of the Asn22 (eelFSHβΔ22/α) and Asn5.22 (eelFSHβΔ5.22/α) N-linked oligosaccharide chain in the eelFSH β-subunit completely reduced the secretion level in the medium and cell lysate of CHO-K1 cells.
30836102	10	7	from	cells	1651:1655	arg1	weight					1598:1603	a molecular weight	1586:1603	a molecular weight of approximately 7-10 kDa in CHO-K1 and CHO-S cells	1586:1655	The oligosaccharide chains in rec-eelFSHβ/α wild-type were modified to a molecular weight of approximately 7-10 kDa in CHO-K1 and CHO-S cells.
30836102	4	8	theme	sites	699:703	arg1	Mutagenesis					645:655	Mutagenesis	645:655	Mutagenesis of the individual and double glycosylated sites	645:703	Mutagenesis of the individual and double glycosylated sites was performed to determine the functions of the oligosaccharide chains on signal transduction.
30836102	13	9	from	Asn79	2032:2036	arg1	removal					2000:2006	the removal	1996:2006	the removal of the carbohydrates at Asn79 of the α-subunit (β/αΔ79)	1996:2062	Specifically, the removal of the carbohydrates at Asn79 of the α-subunit (β/αΔ79) was drastically reduced to 53.8% of the wild-type levels in maximum response.
30836102	12	10	theme	type	1962:1965	arg1	%					1940:1940	64%	1938:1940	64%	1938:1940	The EC50 values of Asn56 and Asn5 resulted in a significant decrease in potency to 64% and 53% of the wild type, respectively.
30836102	12	10	theme	type	1962:1965	arg1	%					1948:1948	53%	1946:1948	53% of the wild type	1946:1965	The EC50 values of Asn56 and Asn5 resulted in a significant decrease in potency to 64% and 53% of the wild type, respectively.
30836102	12	10	theme	type	1962:1965	arg1	type					1962:1965	the wild type	1953:1965	the wild type	1953:1965	The EC50 values of Asn56 and Asn5 resulted in a significant decrease in potency to 64% and 53% of the wild type, respectively.
30836102	9	11	link	N-linked	1392:1399	arg1	oligosaccharides					1401:1416	N-linked oligosaccharides	1392:1416	N-linked oligosaccharides using PNGaseF treatment	1392:1440	The digestion of N-linked oligosaccharides using PNGaseF treatment showed that the molecular weights of all mutants were reduced to 27-kDa.
30836102	11	12	theme	cAMP	1801:1804	arg1	transduction					1813:1824	cAMP signal transduction	1801:1824	cAMP signal transduction through the eelFSH receptor	1801:1852	Oligosaccharide site deletions at positions Asn56 and Asn79 on the α-subunit and Asn5 on the β-subunit were found to play an essential role in cAMP signal transduction through the eelFSH receptor.
30836102	15	13	theme	biological	2333:2342	arg1	activity					2344:2351	biological activity	2333:2351	biological activity through the eelFSH receptor	2333:2379	Our data suggest that N-linked oligosaccharide chains play a pivotal role in biological activity through the eelFSH receptor as suggested in similar studies of other mammalian FSH hormones.
30836102	5	14	theme	eelFSHβΔ5.22/α	853:866	arg1	chain					894:898	Asn5.22 (eelFSHβΔ5.22/α) N-linked oligosaccharide chain	844:898	Asn5.22 (eelFSHβΔ5.22/α) N-linked oligosaccharide chain	844:898	The absence of the Asn22 (eelFSHβΔ22/α) and Asn5.22 (eelFSHβΔ5.22/α) N-linked oligosaccharide chain in the eelFSH β-subunit completely reduced the secretion level in the medium and cell lysate of CHO-K1 cells.
30836102	1	15	theme	follicle-stimulating	140:159	arg1	hormone					161:167	Eel follicle-stimulating hormone	136:167	Eel follicle-stimulating hormone (eelFSH)	136:176	Eel follicle-stimulating hormone (eelFSH) is composed of a common α-subunit and a hormone specific β-subunit, both of which contain two N-linked carbohydrate residues.
30836102	1	15	theme	follicle-stimulating	140:159	arg1	eelFSH					170:175	eelFSH	170:175	eelFSH	170:175	Eel follicle-stimulating hormone (eelFSH) is composed of a common α-subunit and a hormone specific β-subunit, both of which contain two N-linked carbohydrate residues.
30836102	4	16	theme	individual	664:673	arg1	sites					699:703	the individual and double glycosylated sites	660:703	the individual and double glycosylated sites	660:703	Mutagenesis of the individual and double glycosylated sites was performed to determine the functions of the oligosaccharide chains on signal transduction.
30836102	1	17	theme	specific	226:233	arg1	β-subunit					235:243	a hormone specific β-subunit	216:243	a hormone specific β-subunit	216:243	Eel follicle-stimulating hormone (eelFSH) is composed of a common α-subunit and a hormone specific β-subunit, both of which contain two N-linked carbohydrate residues.
30836102	8	18	theme	oligosaccharide	1288:1302	arg1	sites					1304:1308	single oligosaccharide sites	1281:1308	single oligosaccharide sites	1281:1308	Mutants (β/αΔ56, β/αΔ79, and βΔ5/α) lacking single oligosaccharide sites showed molecular weights that were reduced by approximately 10%.
30836102	15	19	theme	pivotal	2317:2323	arg1	role					2325:2328	a pivotal role	2315:2328	a pivotal role	2315:2328	Our data suggest that N-linked oligosaccharide chains play a pivotal role in biological activity through the eelFSH receptor as suggested in similar studies of other mammalian FSH hormones.
30836102	15	20	theme	FSH	2432:2434	arg1	hormones					2436:2443	other mammalian FSH hormones	2416:2443	other mammalian FSH hormones	2416:2443	Our data suggest that N-linked oligosaccharide chains play a pivotal role in biological activity through the eelFSH receptor as suggested in similar studies of other mammalian FSH hormones.
30836102	5	21	theme	secretion	947:955	arg1	level					957:961	the secretion level	943:961	the secretion level in the medium	943:975	The absence of the Asn22 (eelFSHβΔ22/α) and Asn5.22 (eelFSHβΔ5.22/α) N-linked oligosaccharide chain in the eelFSH β-subunit completely reduced the secretion level in the medium and cell lysate of CHO-K1 cells.
30836102	15	22	theme	other	2416:2420	arg1	hormones					2436:2443	other mammalian FSH hormones	2416:2443	other mammalian FSH hormones	2416:2443	Our data suggest that N-linked oligosaccharide chains play a pivotal role in biological activity through the eelFSH receptor as suggested in similar studies of other mammalian FSH hormones.
30836102	6	23	theme	wild-type	1045:1053	arg1	levels					1025:1030	The expression levels	1010:1030	The expression levels of eelFSHβ/α wild-type in CHO suspension (CHO-S) cells	1010:1085	The expression levels of eelFSHβ/α wild-type in CHO suspension (CHO-S) cells was approximately 4-fold higher in CHO-k1 cells.
30836102	6	23	theme	wild-type	1045:1053	arg1	higher					1112:1117	higher	1112:1117	higher	1112:1117	The expression levels of eelFSHβ/α wild-type in CHO suspension (CHO-S) cells was approximately 4-fold higher in CHO-k1 cells.
30836102	11	24	from	Asn5	1739:1742	arg1	β-subunit					1751:1759	the β-subunit	1747:1759	the β-subunit	1747:1759	Oligosaccharide site deletions at positions Asn56 and Asn79 on the α-subunit and Asn5 on the β-subunit were found to play an essential role in cAMP signal transduction through the eelFSH receptor.
30836102	0	25	theme	follicle-stimulating	68:87	arg1	hormone					89:95	recombinant eel follicle-stimulating hormone	52:95	recombinant eel follicle-stimulating hormone for secretion and signal transduction	52:133	Site-specific roles of N-linked oligosaccharides in recombinant eel follicle-stimulating hormone for secretion and signal transduction.
30836102	3	26	theme	receptor	630:637	arg1	gene					639:642	the eelFSH receptor gene	619:642	the eelFSH receptor gene	619:642	Expression vectors were constructed and the biological activity of the recombinant hormones (rec-hormones) was characterized using Chinese hamster ovary (CHO) K1 cells expressing the eelFSH receptor gene.
30836102	14	27	from	identification	2177:2190	arg1	FSH					2251:2253	eel FSH	2247:2253	eel FSH	2247:2253	These results have allowed for the identification of the site-specific roles of carbohydrate residues in eel FSH.
30836102	13	28	theme	α-subunit	2045:2053	arg1	Asn79					2032:2036	Asn79	2032:2036	Asn79 of the α-subunit (β/αΔ79)	2032:2062	Specifically, the removal of the carbohydrates at Asn79 of the α-subunit (β/αΔ79) was drastically reduced to 53.8% of the wild-type levels in maximum response.
30836102	5	29	link	N-linked	869:876	arg1	chain					894:898	Asn5.22 (eelFSHβΔ5.22/α) N-linked oligosaccharide chain	844:898	Asn5.22 (eelFSHβΔ5.22/α) N-linked oligosaccharide chain	844:898	The absence of the Asn22 (eelFSHβΔ22/α) and Asn5.22 (eelFSHβΔ5.22/α) N-linked oligosaccharide chain in the eelFSH β-subunit completely reduced the secretion level in the medium and cell lysate of CHO-K1 cells.
30836102	6	30	theme	expression	1014:1023	arg1	levels					1025:1030	The expression levels	1010:1030	The expression levels of eelFSHβ/α wild-type in CHO suspension (CHO-S) cells	1010:1085	The expression levels of eelFSHβ/α wild-type in CHO suspension (CHO-S) cells was approximately 4-fold higher in CHO-k1 cells.
30836102	6	30	theme	expression	1014:1023	arg1	higher					1112:1117	higher	1112:1117	higher	1112:1117	The expression levels of eelFSHβ/α wild-type in CHO suspension (CHO-S) cells was approximately 4-fold higher in CHO-k1 cells.
30836102	15	31	theme	eelFSH	2365:2370	arg1	receptor					2372:2379	the eelFSH receptor	2361:2379	the eelFSH receptor	2361:2379	Our data suggest that N-linked oligosaccharide chains play a pivotal role in biological activity through the eelFSH receptor as suggested in similar studies of other mammalian FSH hormones.
30836102	11	32	from	α-subunit	1725:1733	arg1	β-subunit					1751:1759	the β-subunit	1747:1759	the β-subunit	1747:1759	Oligosaccharide site deletions at positions Asn56 and Asn79 on the α-subunit and Asn5 on the β-subunit were found to play an essential role in cAMP signal transduction through the eelFSH receptor.
30836102	13	33	theme	levels	2114:2119	arg1	levels					2114:2119	the wild-type levels	2100:2119	the wild-type levels	2100:2119	Specifically, the removal of the carbohydrates at Asn79 of the α-subunit (β/αΔ79) was drastically reduced to 53.8% of the wild-type levels in maximum response.
30836102	13	33	theme	levels	2114:2119	arg1	%					2095:2095	53.8%	2091:2095	53.8% of the wild-type levels	2091:2119	Specifically, the removal of the carbohydrates at Asn79 of the α-subunit (β/αΔ79) was drastically reduced to 53.8% of the wild-type levels in maximum response.
30836102	5	34	from	level	957:961	arg1	medium					970:975	the medium	966:975	the medium	966:975	The absence of the Asn22 (eelFSHβΔ22/α) and Asn5.22 (eelFSHβΔ5.22/α) N-linked oligosaccharide chain in the eelFSH β-subunit completely reduced the secretion level in the medium and cell lysate of CHO-K1 cells.
30836102	5	35	theme	cells	1003:1007	arg1	level					957:961	the secretion level	943:961	the secretion level in the medium	943:975	The absence of the Asn22 (eelFSHβΔ22/α) and Asn5.22 (eelFSHβΔ5.22/α) N-linked oligosaccharide chain in the eelFSH β-subunit completely reduced the secretion level in the medium and cell lysate of CHO-K1 cells.
30836102	5	35	theme	cells	1003:1007	arg1	lysate					986:991	cell lysate	981:991	cell lysate of CHO-K1 cells	981:1007	The absence of the Asn22 (eelFSHβΔ22/α) and Asn5.22 (eelFSHβΔ5.22/α) N-linked oligosaccharide chain in the eelFSH β-subunit completely reduced the secretion level in the medium and cell lysate of CHO-K1 cells.
30836102	11	36	from	positions	1692:1700	arg1	deletions					1679:1687	Oligosaccharide site deletions	1658:1687	Oligosaccharide site deletions at positions Asn56 and Asn79 on the α-subunit and Asn5 on the β-subunit	1658:1759	Oligosaccharide site deletions at positions Asn56 and Asn79 on the α-subunit and Asn5 on the β-subunit were found to play an essential role in cAMP signal transduction through the eelFSH receptor.
30836102	11	36	from	positions	1692:1700	arg1	α-subunit					1725:1733	α-subunit	1725:1733	α-subunit	1725:1733	Oligosaccharide site deletions at positions Asn56 and Asn79 on the α-subunit and Asn5 on the β-subunit were found to play an essential role in cAMP signal transduction through the eelFSH receptor.
30836102	11	36	from	positions	1692:1700	arg1	Asn5					1739:1742	Asn5	1739:1742	Asn5	1739:1742	Oligosaccharide site deletions at positions Asn56 and Asn79 on the α-subunit and Asn5 on the β-subunit were found to play an essential role in cAMP signal transduction through the eelFSH receptor.
30836102	6	37	from	levels	1025:1030	arg1	cells					1081:1085	CHO suspension (CHO-S) cells	1058:1085	CHO suspension (CHO-S) cells	1058:1085	The expression levels of eelFSHβ/α wild-type in CHO suspension (CHO-S) cells was approximately 4-fold higher in CHO-k1 cells.
30836102	4	38	theme	chains	769:774	arg1	functions					736:744	the functions	732:744	the functions of the oligosaccharide chains on signal transduction	732:797	Mutagenesis of the individual and double glycosylated sites was performed to determine the functions of the oligosaccharide chains on signal transduction.
30836102	14	39	theme	carbohydrate	2222:2233	arg1	residues					2235:2242	carbohydrate residues	2222:2242	carbohydrate residues	2222:2242	These results have allowed for the identification of the site-specific roles of carbohydrate residues in eel FSH.
30836102	3	40	theme	Expression	440:449	arg1	vectors					451:457	Expression vectors	440:457	Expression vectors	440:457	Expression vectors were constructed and the biological activity of the recombinant hormones (rec-hormones) was characterized using Chinese hamster ovary (CHO) K1 cells expressing the eelFSH receptor gene.
30836102	0	41	theme	Site-specific	0:12	arg1	roles					14:18	Site-specific roles	0:18	Site-specific roles of N-linked oligosaccharides in recombinant eel follicle-stimulating hormone for secretion and signal transduction	0:133	Site-specific roles of N-linked oligosaccharides in recombinant eel follicle-stimulating hormone for secretion and signal transduction.
30836102	1	42	theme	carbohydrate	281:292	arg1	residues					294:301	two N-linked carbohydrate residues	268:301	two N-linked carbohydrate residues	268:301	Eel follicle-stimulating hormone (eelFSH) is composed of a common α-subunit and a hormone specific β-subunit, both of which contain two N-linked carbohydrate residues.
30836102	12	43	theme	significant	1903:1913	arg1	decrease					1915:1922	a significant decrease	1901:1922	a significant decrease in potency to 64% and 53% of the wild type, respectively	1901:1979	The EC50 values of Asn56 and Asn5 resulted in a significant decrease in potency to 64% and 53% of the wild type, respectively.
30836102	4	44	from	chains	769:774	arg1	transduction					786:797	signal transduction	779:797	signal transduction	779:797	Mutagenesis of the individual and double glycosylated sites was performed to determine the functions of the oligosaccharide chains on signal transduction.
30836102	1	45	contain	contain	260:266	arg1	both					246:249	both	246:249	both	246:249	Eel follicle-stimulating hormone (eelFSH) is composed of a common α-subunit and a hormone specific β-subunit, both of which contain two N-linked carbohydrate residues.
30836102	1	45	contain	contain	260:266	arg1	β-subunit					235:243	a hormone specific β-subunit	216:243	a hormone specific β-subunit	216:243	Eel follicle-stimulating hormone (eelFSH) is composed of a common α-subunit and a hormone specific β-subunit, both of which contain two N-linked carbohydrate residues.
30836102	1	45	contain	contain	260:266	arg1	α-subunit					202:210	common α-subunit	195:210	common α-subunit	195:210	Eel follicle-stimulating hormone (eelFSH) is composed of a common α-subunit and a hormone specific β-subunit, both of which contain two N-linked carbohydrate residues.
30836102	1	45	contain	contain	260:266	arg2	residues					294:301	two N-linked carbohydrate residues	268:301	two N-linked carbohydrate residues	268:301	Eel follicle-stimulating hormone (eelFSH) is composed of a common α-subunit and a hormone specific β-subunit, both of which contain two N-linked carbohydrate residues.
30836102	4	46	theme	signal	779:784	arg1	transduction					786:797	signal transduction	779:797	signal transduction	779:797	Mutagenesis of the individual and double glycosylated sites was performed to determine the functions of the oligosaccharide chains on signal transduction.
30836102	14	47	theme	roles	2213:2217	arg1	identification					2177:2190	the identification	2173:2190	the identification of the site-specific roles of carbohydrate residues in eel FSH	2173:2253	These results have allowed for the identification of the site-specific roles of carbohydrate residues in eel FSH.
30836102	3	48	theme	biological	484:493	arg1	activity					495:502	the biological activity	480:502	the biological activity of the recombinant hormones (rec-hormones)	480:545	Expression vectors were constructed and the biological activity of the recombinant hormones (rec-hormones) was characterized using Chinese hamster ovary (CHO) K1 cells expressing the eelFSH receptor gene.
30836102	5	49	theme	Asn5.22	844:850	arg1	chain					894:898	Asn5.22 (eelFSHβΔ5.22/α) N-linked oligosaccharide chain	844:898	Asn5.22 (eelFSHβΔ5.22/α) N-linked oligosaccharide chain	844:898	The absence of the Asn22 (eelFSHβΔ22/α) and Asn5.22 (eelFSHβΔ5.22/α) N-linked oligosaccharide chain in the eelFSH β-subunit completely reduced the secretion level in the medium and cell lysate of CHO-K1 cells.
30836102	12	50	theme	Asn56	1874:1878	arg1	values					1864:1869	The EC50 values	1855:1869	The EC50 values of Asn56 and Asn5	1855:1887	The EC50 values of Asn56 and Asn5 resulted in a significant decrease in potency to 64% and 53% of the wild type, respectively.
30836102	5	51	theme	cell	981:984	arg1	lysate					986:991	cell lysate	981:991	cell lysate of CHO-K1 cells	981:1007	The absence of the Asn22 (eelFSHβΔ22/α) and Asn5.22 (eelFSHβΔ5.22/α) N-linked oligosaccharide chain in the eelFSH β-subunit completely reduced the secretion level in the medium and cell lysate of CHO-K1 cells.
30836102	2	52	theme	eelFSH	422:427	arg1	β-subunit					429:437	the eelFSH β-subunit	418:437	the eelFSH β-subunit	418:437	We characterized the biologically active single chains by fusing the α-subunit to the carboxyl terminal region of the eelFSH β-subunit.
30836102	0	53	theme	oligosaccharides	32:47	arg1	roles					14:18	Site-specific roles	0:18	Site-specific roles of N-linked oligosaccharides in recombinant eel follicle-stimulating hormone for secretion and signal transduction	0:133	Site-specific roles of N-linked oligosaccharides in recombinant eel follicle-stimulating hormone for secretion and signal transduction.
30836102	11	54	dep	positions	1692:1700	arg1	Asn56					1702:1706	Asn56	1702:1706	Asn56	1702:1706	Oligosaccharide site deletions at positions Asn56 and Asn79 on the α-subunit and Asn5 on the β-subunit were found to play an essential role in cAMP signal transduction through the eelFSH receptor.
30836102	11	54	dep	positions	1692:1700	arg1	positions					1692:1700	positions Asn56 and Asn79	1692:1716	positions Asn56 and Asn79 on the α-subunit and Asn5 on the β-subunit	1692:1759	Oligosaccharide site deletions at positions Asn56 and Asn79 on the α-subunit and Asn5 on the β-subunit were found to play an essential role in cAMP signal transduction through the eelFSH receptor.
30836102	11	54	dep	positions	1692:1700	arg1	Asn79					1712:1716	Asn79	1712:1716	Asn79	1712:1716	Oligosaccharide site deletions at positions Asn56 and Asn79 on the α-subunit and Asn5 on the β-subunit were found to play an essential role in cAMP signal transduction through the eelFSH receptor.
30836102	7	55	theme	wild-type	1174:1182	arg1	weight					1150:1155	The molecular weight	1136:1155	The molecular weight of rec-eelFSHβ/α wild-type by western blotting analysis	1136:1211	The molecular weight of rec-eelFSHβ/α wild-type by western blotting analysis was found to be 34 kDa.
30836102	7	55	theme	wild-type	1174:1182	arg1	34 kDa					1229:1234	34 kDa	1229:1234	34 kDa	1229:1234	The molecular weight of rec-eelFSHβ/α wild-type by western blotting analysis was found to be 34 kDa.
30836102	6	56	theme	CHO	1058:1060	arg1	cells					1081:1085	CHO suspension (CHO-S) cells	1058:1085	CHO suspension (CHO-S) cells	1058:1085	The expression levels of eelFSHβ/α wild-type in CHO suspension (CHO-S) cells was approximately 4-fold higher in CHO-k1 cells.
30836102	3	57	theme	recombinant	511:521	arg1	rec-hormones					533:544	rec-hormones	533:544	rec-hormones	533:544	Expression vectors were constructed and the biological activity of the recombinant hormones (rec-hormones) was characterized using Chinese hamster ovary (CHO) K1 cells expressing the eelFSH receptor gene.
30836102	3	57	theme	recombinant	511:521	arg1	hormones					523:530	the recombinant hormones	507:530	the recombinant hormones (rec-hormones)	507:545	Expression vectors were constructed and the biological activity of the recombinant hormones (rec-hormones) was characterized using Chinese hamster ovary (CHO) K1 cells expressing the eelFSH receptor gene.
30836102	0	58	theme	recombinant	52:62	arg1	hormone					89:95	recombinant eel follicle-stimulating hormone	52:95	recombinant eel follicle-stimulating hormone for secretion and signal transduction	52:133	Site-specific roles of N-linked oligosaccharides in recombinant eel follicle-stimulating hormone for secretion and signal transduction.
30836102	15	59	theme	oligosaccharide	2287:2301	arg1	chains					2303:2308	N-linked oligosaccharide chains	2278:2308	N-linked oligosaccharide chains	2278:2308	Our data suggest that N-linked oligosaccharide chains play a pivotal role in biological activity through the eelFSH receptor as suggested in similar studies of other mammalian FSH hormones.
30836102	15	60	theme	N-linked	2278:2285	arg1	chains					2303:2308	N-linked oligosaccharide chains	2278:2308	N-linked oligosaccharide chains	2278:2308	Our data suggest that N-linked oligosaccharide chains play a pivotal role in biological activity through the eelFSH receptor as suggested in similar studies of other mammalian FSH hormones.
30836102	14	61	from	roles	2213:2217	arg1	FSH					2251:2253	eel FSH	2247:2253	eel FSH	2247:2253	These results have allowed for the identification of the site-specific roles of carbohydrate residues in eel FSH.
30836102	9	62	theme	molecular	1458:1466	arg1	weights					1468:1474	the molecular weights	1454:1474	the molecular weights of all mutants	1454:1489	The digestion of N-linked oligosaccharides using PNGaseF treatment showed that the molecular weights of all mutants were reduced to 27-kDa.
30836102	9	63	theme	oligosaccharides	1401:1416	arg1	digestion					1379:1387	The digestion	1375:1387	The digestion of N-linked oligosaccharides using PNGaseF treatment	1375:1440	The digestion of N-linked oligosaccharides using PNGaseF treatment showed that the molecular weights of all mutants were reduced to 27-kDa.
30836102	0	64	theme	eel	64:66	arg1	hormone					89:95	recombinant eel follicle-stimulating hormone	52:95	recombinant eel follicle-stimulating hormone for secretion and signal transduction	52:133	Site-specific roles of N-linked oligosaccharides in recombinant eel follicle-stimulating hormone for secretion and signal transduction.
30836102	10	65	from	7-10 kDa	1622:1629	arg1	cells					1651:1655	CHO-K1 and CHO-S cells	1634:1655	CHO-K1 and CHO-S cells	1634:1655	The oligosaccharide chains in rec-eelFSHβ/α wild-type were modified to a molecular weight of approximately 7-10 kDa in CHO-K1 and CHO-S cells.
30836102	14	66	from	FSH	2251:2253	arg1	identification					2177:2190	the identification	2173:2190	the identification of the site-specific roles of carbohydrate residues in eel FSH	2173:2253	These results have allowed for the identification of the site-specific roles of carbohydrate residues in eel FSH.
30836102	9	67	theme	PNGaseF	1424:1430	arg1	treatment					1432:1440	PNGaseF treatment	1424:1440	PNGaseF treatment	1424:1440	The digestion of N-linked oligosaccharides using PNGaseF treatment showed that the molecular weights of all mutants were reduced to 27-kDa.
30836102	0	68	link	N-linked	23:30	arg1	oligosaccharides					32:47	N-linked oligosaccharides	23:47	N-linked oligosaccharides	23:47	Site-specific roles of N-linked oligosaccharides in recombinant eel follicle-stimulating hormone for secretion and signal transduction.
30836102	11	69	theme	site	1674:1677	arg1	deletions					1679:1687	Oligosaccharide site deletions	1658:1687	Oligosaccharide site deletions at positions Asn56 and Asn79 on the α-subunit and Asn5 on the β-subunit	1658:1759	Oligosaccharide site deletions at positions Asn56 and Asn79 on the α-subunit and Asn5 on the β-subunit were found to play an essential role in cAMP signal transduction through the eelFSH receptor.
30836102	4	70	gly	glycosylated	686:697	arg1	sites					699:703	the individual and double glycosylated sites	660:703	the individual and double glycosylated sites	660:703	Mutagenesis of the individual and double glycosylated sites was performed to determine the functions of the oligosaccharide chains on signal transduction.
30836102	1	71	link	N-linked	272:279	arg1	residues					294:301	two N-linked carbohydrate residues	268:301	two N-linked carbohydrate residues	268:301	Eel follicle-stimulating hormone (eelFSH) is composed of a common α-subunit and a hormone specific β-subunit, both of which contain two N-linked carbohydrate residues.
30836102	13	72	theme	wild-type	2104:2112	arg1	levels					2114:2119	the wild-type levels	2100:2119	the wild-type levels	2100:2119	Specifically, the removal of the carbohydrates at Asn79 of the α-subunit (β/αΔ79) was drastically reduced to 53.8% of the wild-type levels in maximum response.
30836102	10	73	theme	molecular	1588:1596	arg1	weight					1598:1603	a molecular weight	1586:1603	a molecular weight of approximately 7-10 kDa in CHO-K1 and CHO-S cells	1586:1655	The oligosaccharide chains in rec-eelFSHβ/α wild-type were modified to a molecular weight of approximately 7-10 kDa in CHO-K1 and CHO-S cells.
30836102	11	74	dep	α-subunit	1725:1733	arg1	the					1721:1723	the	1721:1723	the	1721:1723	Oligosaccharide site deletions at positions Asn56 and Asn79 on the α-subunit and Asn5 on the β-subunit were found to play an essential role in cAMP signal transduction through the eelFSH receptor.
30836102	5	75	theme	oligosaccharide	878:892	arg1	chain					894:898	Asn5.22 (eelFSHβΔ5.22/α) N-linked oligosaccharide chain	844:898	Asn5.22 (eelFSHβΔ5.22/α) N-linked oligosaccharide chain	844:898	The absence of the Asn22 (eelFSHβΔ22/α) and Asn5.22 (eelFSHβΔ5.22/α) N-linked oligosaccharide chain in the eelFSH β-subunit completely reduced the secretion level in the medium and cell lysate of CHO-K1 cells.
30836102	12	76	from	decrease	1915:1922	arg1	potency					1927:1933	potency	1927:1933	potency	1927:1933	The EC50 values of Asn56 and Asn5 resulted in a significant decrease in potency to 64% and 53% of the wild type, respectively.
30836102	10	77	theme	CHO-K1	1634:1639	arg1	cells					1651:1655	CHO-K1 and CHO-S cells	1634:1655	CHO-K1 and CHO-S cells	1634:1655	The oligosaccharide chains in rec-eelFSHβ/α wild-type were modified to a molecular weight of approximately 7-10 kDa in CHO-K1 and CHO-S cells.
30836102	2	78	theme	active	338:343	arg1	chains					352:357	the biologically active single chains	321:357	the biologically active single chains	321:357	We characterized the biologically active single chains by fusing the α-subunit to the carboxyl terminal region of the eelFSH β-subunit.
30836102	1	79	theme	common	195:200	arg1	α-subunit					202:210	common α-subunit	195:210	common α-subunit	195:210	Eel follicle-stimulating hormone (eelFSH) is composed of a common α-subunit and a hormone specific β-subunit, both of which contain two N-linked carbohydrate residues.
30836102	15	80	link	N-linked	2278:2285	arg1	chains					2303:2308	N-linked oligosaccharide chains	2278:2308	N-linked oligosaccharide chains	2278:2308	Our data suggest that N-linked oligosaccharide chains play a pivotal role in biological activity through the eelFSH receptor as suggested in similar studies of other mammalian FSH hormones.
30836102	4	81	theme	glycosylated	686:697	arg1	sites					699:703	the individual and double glycosylated sites	660:703	the individual and double glycosylated sites	660:703	Mutagenesis of the individual and double glycosylated sites was performed to determine the functions of the oligosaccharide chains on signal transduction.
30836102	10	82	theme	7-10 kDa	1622:1629	arg1	weight					1598:1603	a molecular weight	1586:1603	a molecular weight of approximately 7-10 kDa in CHO-K1 and CHO-S cells	1586:1655	The oligosaccharide chains in rec-eelFSHβ/α wild-type were modified to a molecular weight of approximately 7-10 kDa in CHO-K1 and CHO-S cells.
30836102	9	83	theme	mutants	1483:1489	arg1	weights					1468:1474	the molecular weights	1454:1474	the molecular weights of all mutants	1454:1489	The digestion of N-linked oligosaccharides using PNGaseF treatment showed that the molecular weights of all mutants were reduced to 27-kDa.
30836102	12	84	theme	wild	1957:1960	arg1	type					1962:1965	the wild type	1953:1965	the wild type	1953:1965	The EC50 values of Asn56 and Asn5 resulted in a significant decrease in potency to 64% and 53% of the wild type, respectively.
30836102	11	85	theme	signal	1806:1811	arg1	transduction					1813:1824	cAMP signal transduction	1801:1824	cAMP signal transduction through the eelFSH receptor	1801:1852	Oligosaccharide site deletions at positions Asn56 and Asn79 on the α-subunit and Asn5 on the β-subunit were found to play an essential role in cAMP signal transduction through the eelFSH receptor.
30836102	14	86	theme	eel	2247:2249	arg1	FSH					2251:2253	eel FSH	2247:2253	eel FSH	2247:2253	These results have allowed for the identification of the site-specific roles of carbohydrate residues in eel FSH.
30836102	1	87	theme	hormone	218:224	arg1	β-subunit					235:243	a hormone specific β-subunit	216:243	a hormone specific β-subunit	216:243	Eel follicle-stimulating hormone (eelFSH) is composed of a common α-subunit and a hormone specific β-subunit, both of which contain two N-linked carbohydrate residues.
30836102	10	88	theme	CHO-S	1645:1649	arg1	cells					1651:1655	CHO-K1 and CHO-S cells	1634:1655	CHO-K1 and CHO-S cells	1634:1655	The oligosaccharide chains in rec-eelFSHβ/α wild-type were modified to a molecular weight of approximately 7-10 kDa in CHO-K1 and CHO-S cells.
30836102	3	89	theme	K1	599:600	arg1	cells					602:606	Chinese hamster ovary (CHO) K1 cells	571:606	Chinese hamster ovary (CHO) K1 cells expressing the eelFSH receptor gene	571:642	Expression vectors were constructed and the biological activity of the recombinant hormones (rec-hormones) was characterized using Chinese hamster ovary (CHO) K1 cells expressing the eelFSH receptor gene.
30836102	1	90	theme	Eel	136:138	arg1	hormone					161:167	Eel follicle-stimulating hormone	136:167	Eel follicle-stimulating hormone (eelFSH)	136:176	Eel follicle-stimulating hormone (eelFSH) is composed of a common α-subunit and a hormone specific β-subunit, both of which contain two N-linked carbohydrate residues.
30836102	1	90	theme	Eel	136:138	arg1	eelFSH					170:175	eelFSH	170:175	eelFSH	170:175	Eel follicle-stimulating hormone (eelFSH) is composed of a common α-subunit and a hormone specific β-subunit, both of which contain two N-linked carbohydrate residues.
30836102	8	91	theme	single	1281:1286	arg1	sites					1304:1308	single oligosaccharide sites	1281:1308	single oligosaccharide sites	1281:1308	Mutants (β/αΔ56, β/αΔ79, and βΔ5/α) lacking single oligosaccharide sites showed molecular weights that were reduced by approximately 10%.
30836102	11	92	theme	eelFSH	1838:1843	arg1	receptor					1845:1852	the eelFSH receptor	1834:1852	the eelFSH receptor	1834:1852	Oligosaccharide site deletions at positions Asn56 and Asn79 on the α-subunit and Asn5 on the β-subunit were found to play an essential role in cAMP signal transduction through the eelFSH receptor.
30836102	5	93	from	absence	804:810	arg1	β-subunit					914:922	the eelFSH β-subunit	903:922	the eelFSH β-subunit	903:922	The absence of the Asn22 (eelFSHβΔ22/α) and Asn5.22 (eelFSHβΔ5.22/α) N-linked oligosaccharide chain in the eelFSH β-subunit completely reduced the secretion level in the medium and cell lysate of CHO-K1 cells.
30836102	15	94	theme	hormones	2436:2443	arg1	studies					2405:2411	similar studies	2397:2411	similar studies of other mammalian FSH hormones	2397:2443	Our data suggest that N-linked oligosaccharide chains play a pivotal role in biological activity through the eelFSH receptor as suggested in similar studies of other mammalian FSH hormones.
30836102	3	95	theme	eelFSH	623:628	arg1	gene					639:642	the eelFSH receptor gene	619:642	the eelFSH receptor gene	619:642	Expression vectors were constructed and the biological activity of the recombinant hormones (rec-hormones) was characterized using Chinese hamster ovary (CHO) K1 cells expressing the eelFSH receptor gene.
30836102	15	96	theme	mammalian	2422:2430	arg1	hormones					2436:2443	other mammalian FSH hormones	2416:2443	other mammalian FSH hormones	2416:2443	Our data suggest that N-linked oligosaccharide chains play a pivotal role in biological activity through the eelFSH receptor as suggested in similar studies of other mammalian FSH hormones.
30836102	5	97	theme	eelFSH	907:912	arg1	β-subunit					914:922	the eelFSH β-subunit	903:922	the eelFSH β-subunit	903:922	The absence of the Asn22 (eelFSHβΔ22/α) and Asn5.22 (eelFSHβΔ5.22/α) N-linked oligosaccharide chain in the eelFSH β-subunit completely reduced the secretion level in the medium and cell lysate of CHO-K1 cells.
30836102	6	98	theme	eelFSHβ/α	1035:1043	arg1	wild-type					1045:1053	eelFSHβ/α wild-type	1035:1053	eelFSHβ/α wild-type	1035:1053	The expression levels of eelFSHβ/α wild-type in CHO suspension (CHO-S) cells was approximately 4-fold higher in CHO-k1 cells.
30836102	8	99	theme	molecular	1317:1325	arg1	weights					1327:1333	molecular weights	1317:1333	molecular weights that were reduced by approximately 10%	1317:1372	Mutants (β/αΔ56, β/αΔ79, and βΔ5/α) lacking single oligosaccharide sites showed molecular weights that were reduced by approximately 10%.
30836102	15	100	theme	similar	2397:2403	arg1	studies					2405:2411	similar studies	2397:2411	similar studies of other mammalian FSH hormones	2397:2443	Our data suggest that N-linked oligosaccharide chains play a pivotal role in biological activity through the eelFSH receptor as suggested in similar studies of other mammalian FSH hormones.
30836102	10	101	from	chains	1535:1540	arg1	wild-type					1559:1567	rec-eelFSHβ/α wild-type	1545:1567	rec-eelFSHβ/α wild-type	1545:1567	The oligosaccharide chains in rec-eelFSHβ/α wild-type were modified to a molecular weight of approximately 7-10 kDa in CHO-K1 and CHO-S cells.
30836102	0	102	from	roles	14:18	arg1	hormone					89:95	recombinant eel follicle-stimulating hormone	52:95	recombinant eel follicle-stimulating hormone for secretion and signal transduction	52:133	Site-specific roles of N-linked oligosaccharides in recombinant eel follicle-stimulating hormone for secretion and signal transduction.
30836102	0	103	theme	signal	115:120	arg1	transduction					122:133	signal transduction	115:133	signal transduction	115:133	Site-specific roles of N-linked oligosaccharides in recombinant eel follicle-stimulating hormone for secretion and signal transduction.
30836102	12	104	theme	Asn5	1884:1887	arg1	values					1864:1869	The EC50 values	1855:1869	The EC50 values of Asn56 and Asn5	1855:1887	The EC50 values of Asn56 and Asn5 resulted in a significant decrease in potency to 64% and 53% of the wild type, respectively.
30836102	11	105	theme	essential	1783:1791	arg1	role					1793:1796	an essential role	1780:1796	an essential role	1780:1796	Oligosaccharide site deletions at positions Asn56 and Asn79 on the α-subunit and Asn5 on the β-subunit were found to play an essential role in cAMP signal transduction through the eelFSH receptor.
30836102	13	106	theme	carbohydrates	2015:2027	arg1	removal					2000:2006	the removal	1996:2006	the removal of the carbohydrates at Asn79 of the α-subunit (β/αΔ79)	1996:2062	Specifically, the removal of the carbohydrates at Asn79 of the α-subunit (β/αΔ79) was drastically reduced to 53.8% of the wild-type levels in maximum response.
30836102	4	107	from	transduction	786:797	arg1	functions					736:744	the functions	732:744	the functions of the oligosaccharide chains on signal transduction	732:797	Mutagenesis of the individual and double glycosylated sites was performed to determine the functions of the oligosaccharide chains on signal transduction.
30836102	5	108	theme	CHO-K1	996:1001	arg1	cells					1003:1007	CHO-K1 cells	996:1007	CHO-K1 cells	996:1007	The absence of the Asn22 (eelFSHβΔ22/α) and Asn5.22 (eelFSHβΔ5.22/α) N-linked oligosaccharide chain in the eelFSH β-subunit completely reduced the secretion level in the medium and cell lysate of CHO-K1 cells.
30836102	13	109	theme	maximum	2124:2130	arg1	response					2132:2139	maximum response	2124:2139	maximum response	2124:2139	Specifically, the removal of the carbohydrates at Asn79 of the α-subunit (β/αΔ79) was drastically reduced to 53.8% of the wild-type levels in maximum response.
30836102	14	110	theme	residues	2235:2242	arg1	roles					2213:2217	the site-specific roles	2195:2217	the site-specific roles of carbohydrate residues in eel FSH	2195:2253	These results have allowed for the identification of the site-specific roles of carbohydrate residues in eel FSH.
30836102	7	111	theme	blotting	1195:1202	arg1	analysis					1204:1211	western blotting analysis	1187:1211	western blotting analysis	1187:1211	The molecular weight of rec-eelFSHβ/α wild-type by western blotting analysis was found to be 34 kDa.
30836102	10	112	from	weight	1598:1603	arg1	cells					1651:1655	CHO-K1 and CHO-S cells	1634:1655	CHO-K1 and CHO-S cells	1634:1655	The oligosaccharide chains in rec-eelFSHβ/α wild-type were modified to a molecular weight of approximately 7-10 kDa in CHO-K1 and CHO-S cells.
30836102	4	113	theme	oligosaccharide	753:767	arg1	chains					769:774	the oligosaccharide chains	749:774	the oligosaccharide chains on signal transduction	749:797	Mutagenesis of the individual and double glycosylated sites was performed to determine the functions of the oligosaccharide chains on signal transduction.
30836102	2	114	theme	carboxyl	390:397	arg1	region					408:413	the carboxyl terminal region	386:413	the carboxyl terminal region of the eelFSH β-subunit	386:437	We characterized the biologically active single chains by fusing the α-subunit to the carboxyl terminal region of the eelFSH β-subunit.
30836102	0	115	theme	N-linked	23:30	arg1	oligosaccharides					32:47	N-linked oligosaccharides	23:47	N-linked oligosaccharides	23:47	Site-specific roles of N-linked oligosaccharides in recombinant eel follicle-stimulating hormone for secretion and signal transduction.
30836102	6	116	theme	CHO-S	1074:1078	arg1	cells					1081:1085	CHO suspension (CHO-S) cells	1058:1085	CHO suspension (CHO-S) cells	1058:1085	The expression levels of eelFSHβ/α wild-type in CHO suspension (CHO-S) cells was approximately 4-fold higher in CHO-k1 cells.
30836102	8	117	dep	Mutants	1237:1243	arg1	β/αΔ79					1254:1259	β/αΔ79	1254:1259	β/αΔ79	1254:1259	Mutants (β/αΔ56, β/αΔ79, and βΔ5/α) lacking single oligosaccharide sites showed molecular weights that were reduced by approximately 10%.
30836102	8	117	dep	Mutants	1237:1243	arg1	βΔ5/α					1266:1270	βΔ5/α	1266:1270	βΔ5/α	1266:1270	Mutants (β/αΔ56, β/αΔ79, and βΔ5/α) lacking single oligosaccharide sites showed molecular weights that were reduced by approximately 10%.
30836102	8	117	dep	Mutants	1237:1243	arg1	β/αΔ56					1246:1251	β/αΔ56	1246:1251	β/αΔ56	1246:1251	Mutants (β/αΔ56, β/αΔ79, and βΔ5/α) lacking single oligosaccharide sites showed molecular weights that were reduced by approximately 10%.
30836102	14	118	theme	site-specific	2199:2211	arg1	roles					2213:2217	the site-specific roles	2195:2217	the site-specific roles of carbohydrate residues in eel FSH	2195:2253	These results have allowed for the identification of the site-specific roles of carbohydrate residues in eel FSH.
30836102	2	119	theme	β-subunit	429:437	arg1	region					408:413	the carboxyl terminal region	386:413	the carboxyl terminal region of the eelFSH β-subunit	386:437	We characterized the biologically active single chains by fusing the α-subunit to the carboxyl terminal region of the eelFSH β-subunit.
30836102	7	120	theme	rec-eelFSHβ/α	1160:1172	arg1	wild-type					1174:1182	rec-eelFSHβ/α wild-type	1160:1182	rec-eelFSHβ/α wild-type	1160:1182	The molecular weight of rec-eelFSHβ/α wild-type by western blotting analysis was found to be 34 kDa.
30836102	13	121	attach	removal	2000:2006	arg3	Asn79					2032:2036	Asn79	2032:2036	Asn79 of the α-subunit (β/αΔ79)	2032:2062	Specifically, the removal of the carbohydrates at Asn79 of the α-subunit (β/αΔ79) was drastically reduced to 53.8% of the wild-type levels in maximum response.
30836102	13	121	attach	removal	2000:2006	arg2	carbohydrates					2015:2027	the carbohydrates	2011:2027	the carbohydrates	2011:2027	Specifically, the removal of the carbohydrates at Asn79 of the α-subunit (β/αΔ79) was drastically reduced to 53.8% of the wild-type levels in maximum response.
30836102	6	122	theme	suspension	1062:1071	arg1	cells					1081:1085	CHO suspension (CHO-S) cells	1058:1085	CHO suspension (CHO-S) cells	1058:1085	The expression levels of eelFSHβ/α wild-type in CHO suspension (CHO-S) cells was approximately 4-fold higher in CHO-k1 cells.
30836102	1	123	dep	α-subunit	202:210	arg1	a					193:193	a	193:193	a	193:193	Eel follicle-stimulating hormone (eelFSH) is composed of a common α-subunit and a hormone specific β-subunit, both of which contain two N-linked carbohydrate residues.
30836102	5	124	theme	Asn22	819:823	arg1	absence					804:810	The absence	800:810	The absence of the Asn22 (eelFSHβΔ22/α) and Asn5.22 (eelFSHβΔ5.22/α) N-linked oligosaccharide chain in the eelFSH β-subunit	800:922	The absence of the Asn22 (eelFSHβΔ22/α) and Asn5.22 (eelFSHβΔ5.22/α) N-linked oligosaccharide chain in the eelFSH β-subunit completely reduced the secretion level in the medium and cell lysate of CHO-K1 cells.
30836102	12	125	theme	EC50	1859:1862	arg1	values					1864:1869	The EC50 values	1855:1869	The EC50 values of Asn56 and Asn5	1855:1887	The EC50 values of Asn56 and Asn5 resulted in a significant decrease in potency to 64% and 53% of the wild type, respectively.
30836102	3	126	theme	hormones	523:530	arg1	activity					495:502	the biological activity	480:502	the biological activity of the recombinant hormones (rec-hormones)	480:545	Expression vectors were constructed and the biological activity of the recombinant hormones (rec-hormones) was characterized using Chinese hamster ovary (CHO) K1 cells expressing the eelFSH receptor gene.
30836102	10	127	theme	oligosaccharide	1519:1533	arg1	chains					1535:1540	The oligosaccharide chains	1515:1540	The oligosaccharide chains in rec-eelFSHβ/α wild-type	1515:1567	The oligosaccharide chains in rec-eelFSHβ/α wild-type were modified to a molecular weight of approximately 7-10 kDa in CHO-K1 and CHO-S cells.
30836102	2	128	theme	single	345:350	arg1	chains					352:357	the biologically active single chains	321:357	the biologically active single chains	321:357	We characterized the biologically active single chains by fusing the α-subunit to the carboxyl terminal region of the eelFSH β-subunit.
30836102	4	129	from	functions	736:744	arg1	transduction					786:797	signal transduction	779:797	signal transduction	779:797	Mutagenesis of the individual and double glycosylated sites was performed to determine the functions of the oligosaccharide chains on signal transduction.
30836102	9	130	theme	N-linked	1392:1399	arg1	oligosaccharides					1401:1416	N-linked oligosaccharides	1392:1416	N-linked oligosaccharides using PNGaseF treatment	1392:1440	The digestion of N-linked oligosaccharides using PNGaseF treatment showed that the molecular weights of all mutants were reduced to 27-kDa.
30836102	5	131	theme	N-linked	869:876	arg1	chain					894:898	Asn5.22 (eelFSHβΔ5.22/α) N-linked oligosaccharide chain	844:898	Asn5.22 (eelFSHβΔ5.22/α) N-linked oligosaccharide chain	844:898	The absence of the Asn22 (eelFSHβΔ22/α) and Asn5.22 (eelFSHβΔ5.22/α) N-linked oligosaccharide chain in the eelFSH β-subunit completely reduced the secretion level in the medium and cell lysate of CHO-K1 cells.
30836102	6	132	theme	CHO-k1	1122:1127	arg1	cells					1129:1133	CHO-k1 cells	1122:1133	CHO-k1 cells	1122:1133	The expression levels of eelFSHβ/α wild-type in CHO suspension (CHO-S) cells was approximately 4-fold higher in CHO-k1 cells.
30836102	7	133	theme	western	1187:1193	arg1	analysis					1204:1211	western blotting analysis	1187:1211	western blotting analysis	1187:1211	The molecular weight of rec-eelFSHβ/α wild-type by western blotting analysis was found to be 34 kDa.
31750645	4	0	theme	low	976:978	arg1	μL-1					1007:1010	0.01 fmol μL-1	997:1010	0.01 fmol μL-1	997:1010	The as-prepared MCNC@COF@GSH microspheres possessed fast magnetic responsiveness, regular porosity, large surface areas, and good hydrophilicity, resulting in remarkable performances in N-linked glycopeptide enrichment with low detection limit (0.01 fmol μL-1), high selectivity (1:5000, human immunoglobulin G (IgG) digests to bovine serum albumin digests), excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500), and reusability (at least five times).
31750645	4	0	theme	low	976:978	arg1	limit					990:994	low detection limit	976:994	low detection limit (0.01 fmol μL-1)	976:1011	The as-prepared MCNC@COF@GSH microspheres possessed fast magnetic responsiveness, regular porosity, large surface areas, and good hydrophilicity, resulting in remarkable performances in N-linked glycopeptide enrichment with low detection limit (0.01 fmol μL-1), high selectivity (1:5000, human immunoglobulin G (IgG) digests to bovine serum albumin digests), excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500), and reusability (at least five times).
31750645	6	1	dep	providing	1520:1528	arg1	addition					1508:1515	addition	1508:1515	addition	1508:1515	In addition to providing a strategy for versatile functionalization of magnetic covalent organic frameworks (COFs), this study may be used to develop application of endogenous glycoproteome analysis.
31750645	3	2	link	N-linked	728:735	arg1	glycopeptides					737:749	N-linked glycopeptides	728:749	N-linked glycopeptides	728:749	Reported here is a novel glutathione-functionalized magnetic covalent organic framework microsphere (denoted as MCNC@COF@GSH) endowed with size-exclusion effect and strong hydrophilicity for selective and efficient enrichment of N-linked glycopeptides.
31750645	5	3	theme	N-linked	1453:1460	arg1	enrichment					1475:1484	endogenous N-linked glycopeptide enrichment	1442:1484	endogenous N-linked glycopeptide enrichment from human saliva	1442:1502	More excitingly, 143 endogenous N-linked glycopeptides were clearly identified from 10 μL sample of human saliva treated with the MCNC@COF@GSH microspheres, which is the unprecedented high efficiency in endogenous N-linked glycopeptide enrichment from human saliva.
31750645	2	4	theme	complex	405:411	arg1	matrices					424:431	complex biological matrices	405:431	complex biological matrices	405:431	The low abundance of endogenous glycopeptides and abundant interferents (e.g., large-size proteins and heteropeptides) in complex biological matrices render the direct analysis of endogenous glycopeptides difficult.
31750645	4	5	theme	size-exclusion	1121:1134	arg1	albumin					1173:1179	IgG digests/IgG/bovine serum albumin	1144:1179	IgG digests/IgG/bovine serum albumin (BSA)	1144:1185	The as-prepared MCNC@COF@GSH microspheres possessed fast magnetic responsiveness, regular porosity, large surface areas, and good hydrophilicity, resulting in remarkable performances in N-linked glycopeptide enrichment with low detection limit (0.01 fmol μL-1), high selectivity (1:5000, human immunoglobulin G (IgG) digests to bovine serum albumin digests), excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500), and reusability (at least five times).
31750645	4	5	theme	size-exclusion	1121:1134	arg1	effect					1136:1141	excellent size-exclusion effect	1111:1141	excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500)	1111:1197	The as-prepared MCNC@COF@GSH microspheres possessed fast magnetic responsiveness, regular porosity, large surface areas, and good hydrophilicity, resulting in remarkable performances in N-linked glycopeptide enrichment with low detection limit (0.01 fmol μL-1), high selectivity (1:5000, human immunoglobulin G (IgG) digests to bovine serum albumin digests), excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500), and reusability (at least five times).
31750645	5	6	link	N-linked	1453:1460	arg1	enrichment					1475:1484	endogenous N-linked glycopeptide enrichment	1442:1484	endogenous N-linked glycopeptide enrichment from human saliva	1442:1502	More excitingly, 143 endogenous N-linked glycopeptides were clearly identified from 10 μL sample of human saliva treated with the MCNC@COF@GSH microspheres, which is the unprecedented high efficiency in endogenous N-linked glycopeptide enrichment from human saliva.
31750645	2	7	theme	interferents	342:353	arg1	abundance					291:299	The low abundance	283:299	The low abundance of endogenous glycopeptides and abundant interferents (e.g., large-size proteins and heteropeptides) in complex biological matrices	283:431	The low abundance of endogenous glycopeptides and abundant interferents (e.g., large-size proteins and heteropeptides) in complex biological matrices render the direct analysis of endogenous glycopeptides difficult.
31750645	6	8	theme	organic	1594:1600	arg1	COFs					1614:1617	COFs	1614:1617	COFs	1614:1617	In addition to providing a strategy for versatile functionalization of magnetic covalent organic frameworks (COFs), this study may be used to develop application of endogenous glycoproteome analysis.
31750645	6	8	theme	organic	1594:1600	arg1	frameworks					1602:1611	magnetic covalent organic frameworks	1576:1611	magnetic covalent organic frameworks (COFs)	1576:1618	In addition to providing a strategy for versatile functionalization of magnetic covalent organic frameworks (COFs), this study may be used to develop application of endogenous glycoproteome analysis.
31750645	4	9	theme	N-linked	938:945	arg1	enrichment					960:969	N-linked glycopeptide enrichment	938:969	N-linked glycopeptide enrichment	938:969	The as-prepared MCNC@COF@GSH microspheres possessed fast magnetic responsiveness, regular porosity, large surface areas, and good hydrophilicity, resulting in remarkable performances in N-linked glycopeptide enrichment with low detection limit (0.01 fmol μL-1), high selectivity (1:5000, human immunoglobulin G (IgG) digests to bovine serum albumin digests), excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500), and reusability (at least five times).
31750645	4	10	theme	as-prepared	756:766	arg1	microspheres					781:792	The as-prepared MCNC@COF@GSH microspheres	752:792	The as-prepared MCNC@COF@GSH microspheres	752:792	The as-prepared MCNC@COF@GSH microspheres possessed fast magnetic responsiveness, regular porosity, large surface areas, and good hydrophilicity, resulting in remarkable performances in N-linked glycopeptide enrichment with low detection limit (0.01 fmol μL-1), high selectivity (1:5000, human immunoglobulin G (IgG) digests to bovine serum albumin digests), excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500), and reusability (at least five times).
31750645	4	11	contain	possessed	794:802	arg2	responsiveness					818:831	fast magnetic responsiveness	804:831	fast magnetic responsiveness	804:831	The as-prepared MCNC@COF@GSH microspheres possessed fast magnetic responsiveness, regular porosity, large surface areas, and good hydrophilicity, resulting in remarkable performances in N-linked glycopeptide enrichment with low detection limit (0.01 fmol μL-1), high selectivity (1:5000, human immunoglobulin G (IgG) digests to bovine serum albumin digests), excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500), and reusability (at least five times).
31750645	4	11	contain	possessed	794:802	arg2	porosity					842:849	regular porosity	834:849	regular porosity	834:849	The as-prepared MCNC@COF@GSH microspheres possessed fast magnetic responsiveness, regular porosity, large surface areas, and good hydrophilicity, resulting in remarkable performances in N-linked glycopeptide enrichment with low detection limit (0.01 fmol μL-1), high selectivity (1:5000, human immunoglobulin G (IgG) digests to bovine serum albumin digests), excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500), and reusability (at least five times).
31750645	4	11	contain	possessed	794:802	arg1	microspheres					781:792	The as-prepared MCNC@COF@GSH microspheres	752:792	The as-prepared MCNC@COF@GSH microspheres	752:792	The as-prepared MCNC@COF@GSH microspheres possessed fast magnetic responsiveness, regular porosity, large surface areas, and good hydrophilicity, resulting in remarkable performances in N-linked glycopeptide enrichment with low detection limit (0.01 fmol μL-1), high selectivity (1:5000, human immunoglobulin G (IgG) digests to bovine serum albumin digests), excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500), and reusability (at least five times).
31750645	4	11	contain	possessed	794:802	arg2	areas					866:870	large surface areas	852:870	large surface areas	852:870	The as-prepared MCNC@COF@GSH microspheres possessed fast magnetic responsiveness, regular porosity, large surface areas, and good hydrophilicity, resulting in remarkable performances in N-linked glycopeptide enrichment with low detection limit (0.01 fmol μL-1), high selectivity (1:5000, human immunoglobulin G (IgG) digests to bovine serum albumin digests), excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500), and reusability (at least five times).
31750645	4	11	contain	possessed	794:802	arg2	hydrophilicity					882:895	good hydrophilicity	877:895	good hydrophilicity	877:895	The as-prepared MCNC@COF@GSH microspheres possessed fast magnetic responsiveness, regular porosity, large surface areas, and good hydrophilicity, resulting in remarkable performances in N-linked glycopeptide enrichment with low detection limit (0.01 fmol μL-1), high selectivity (1:5000, human immunoglobulin G (IgG) digests to bovine serum albumin digests), excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500), and reusability (at least five times).
31750645	5	12	theme	high	1423:1426	arg1	microspheres					1382:1393	the MCNC@COF@GSH microspheres	1365:1393	the MCNC@COF@GSH microspheres	1365:1393	More excitingly, 143 endogenous N-linked glycopeptides were clearly identified from 10 μL sample of human saliva treated with the MCNC@COF@GSH microspheres, which is the unprecedented high efficiency in endogenous N-linked glycopeptide enrichment from human saliva.
31750645	5	12	theme	high	1423:1426	arg1	efficiency					1428:1437	the unprecedented high efficiency	1405:1437	the unprecedented high efficiency in endogenous N-linked glycopeptide enrichment from human saliva	1405:1502	More excitingly, 143 endogenous N-linked glycopeptides were clearly identified from 10 μL sample of human saliva treated with the MCNC@COF@GSH microspheres, which is the unprecedented high efficiency in endogenous N-linked glycopeptide enrichment from human saliva.
31750645	3	13	theme	@	619:619	arg1	GSH					620:622	MCNC@COF@GSH	611:622	MCNC@COF@GSH	611:622	Reported here is a novel glutathione-functionalized magnetic covalent organic framework microsphere (denoted as MCNC@COF@GSH) endowed with size-exclusion effect and strong hydrophilicity for selective and efficient enrichment of N-linked glycopeptides.
31750645	6	14	theme	magnetic	1576:1583	arg1	COFs					1614:1617	COFs	1614:1617	COFs	1614:1617	In addition to providing a strategy for versatile functionalization of magnetic covalent organic frameworks (COFs), this study may be used to develop application of endogenous glycoproteome analysis.
31750645	6	14	theme	magnetic	1576:1583	arg1	frameworks					1602:1611	magnetic covalent organic frameworks	1576:1611	magnetic covalent organic frameworks (COFs)	1576:1618	In addition to providing a strategy for versatile functionalization of magnetic covalent organic frameworks (COFs), this study may be used to develop application of endogenous glycoproteome analysis.
31750645	1	15	theme	significant	208:218	arg1	role					220:223	a significant role	206:223	a significant role	206:223	Endogenous glycopeptides have been confirmed to play a significant role in multifarious pathological and physiological processes.
31750645	4	16	theme	@	772:772	arg1	microspheres					781:792	The as-prepared MCNC@COF@GSH microspheres	752:792	The as-prepared MCNC@COF@GSH microspheres	752:792	The as-prepared MCNC@COF@GSH microspheres possessed fast magnetic responsiveness, regular porosity, large surface areas, and good hydrophilicity, resulting in remarkable performances in N-linked glycopeptide enrichment with low detection limit (0.01 fmol μL-1), high selectivity (1:5000, human immunoglobulin G (IgG) digests to bovine serum albumin digests), excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500), and reusability (at least five times).
31750645	2	17	gly	glycopeptides	315:327	arg2	glycopeptides					315:327	endogenous glycopeptides	304:327	endogenous glycopeptides	304:327	The low abundance of endogenous glycopeptides and abundant interferents (e.g., large-size proteins and heteropeptides) in complex biological matrices render the direct analysis of endogenous glycopeptides difficult.
31750645	2	18	theme	large-size	362:371	arg1	proteins					373:380	large-size proteins	362:380	large-size proteins	362:380	The low abundance of endogenous glycopeptides and abundant interferents (e.g., large-size proteins and heteropeptides) in complex biological matrices render the direct analysis of endogenous glycopeptides difficult.
31750645	1	19	theme	pathological	241:252	arg1	processes					272:280	multifarious pathological and physiological processes	228:280	multifarious pathological and physiological processes	228:280	Endogenous glycopeptides have been confirmed to play a significant role in multifarious pathological and physiological processes.
31750645	5	20	from	saliva	1497:1502	arg1	microspheres					1382:1393	the MCNC@COF@GSH microspheres	1365:1393	the MCNC@COF@GSH microspheres	1365:1393	More excitingly, 143 endogenous N-linked glycopeptides were clearly identified from 10 μL sample of human saliva treated with the MCNC@COF@GSH microspheres, which is the unprecedented high efficiency in endogenous N-linked glycopeptide enrichment from human saliva.
31750645	5	20	from	saliva	1497:1502	arg1	enrichment					1475:1484	endogenous N-linked glycopeptide enrichment	1442:1484	endogenous N-linked glycopeptide enrichment from human saliva	1442:1502	More excitingly, 143 endogenous N-linked glycopeptides were clearly identified from 10 μL sample of human saliva treated with the MCNC@COF@GSH microspheres, which is the unprecedented high efficiency in endogenous N-linked glycopeptide enrichment from human saliva.
31750645	5	20	from	saliva	1497:1502	arg1	efficiency					1428:1437	the unprecedented high efficiency	1405:1437	the unprecedented high efficiency in endogenous N-linked glycopeptide enrichment from human saliva	1405:1502	More excitingly, 143 endogenous N-linked glycopeptides were clearly identified from 10 μL sample of human saliva treated with the MCNC@COF@GSH microspheres, which is the unprecedented high efficiency in endogenous N-linked glycopeptide enrichment from human saliva.
31750645	1	21	gly	glycopeptides	164:176	arg2	glycopeptides					164:176	Endogenous glycopeptides	153:176	Endogenous glycopeptides	153:176	Endogenous glycopeptides have been confirmed to play a significant role in multifarious pathological and physiological processes.
31750645	4	22	from	performances	922:933	arg1	enrichment					960:969	N-linked glycopeptide enrichment	938:969	N-linked glycopeptide enrichment	938:969	The as-prepared MCNC@COF@GSH microspheres possessed fast magnetic responsiveness, regular porosity, large surface areas, and good hydrophilicity, resulting in remarkable performances in N-linked glycopeptide enrichment with low detection limit (0.01 fmol μL-1), high selectivity (1:5000, human immunoglobulin G (IgG) digests to bovine serum albumin digests), excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500), and reusability (at least five times).
31750645	1	23	theme	physiological	258:270	arg1	processes					272:280	multifarious pathological and physiological processes	228:280	multifarious pathological and physiological processes	228:280	Endogenous glycopeptides have been confirmed to play a significant role in multifarious pathological and physiological processes.
31750645	4	24	theme	fast	804:807	arg1	responsiveness					818:831	fast magnetic responsiveness	804:831	fast magnetic responsiveness	804:831	The as-prepared MCNC@COF@GSH microspheres possessed fast magnetic responsiveness, regular porosity, large surface areas, and good hydrophilicity, resulting in remarkable performances in N-linked glycopeptide enrichment with low detection limit (0.01 fmol μL-1), high selectivity (1:5000, human immunoglobulin G (IgG) digests to bovine serum albumin digests), excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500), and reusability (at least five times).
31750645	3	25	theme	strong	664:669	arg1	hydrophilicity					671:684	strong hydrophilicity	664:684	strong hydrophilicity	664:684	Reported here is a novel glutathione-functionalized magnetic covalent organic framework microsphere (denoted as MCNC@COF@GSH) endowed with size-exclusion effect and strong hydrophilicity for selective and efficient enrichment of N-linked glycopeptides.
31750645	1	26	theme	Endogenous	153:162	arg1	glycopeptides					164:176	Endogenous glycopeptides	153:176	Endogenous glycopeptides	153:176	Endogenous glycopeptides have been confirmed to play a significant role in multifarious pathological and physiological processes.
31750645	2	27	theme	glycopeptides	315:327	arg1	abundance					291:299	The low abundance	283:299	The low abundance of endogenous glycopeptides and abundant interferents (e.g., large-size proteins and heteropeptides) in complex biological matrices	283:431	The low abundance of endogenous glycopeptides and abundant interferents (e.g., large-size proteins and heteropeptides) in complex biological matrices render the direct analysis of endogenous glycopeptides difficult.
31750645	2	28	dep	proteins	373:380	arg1	e.g.					356:359	e.g.	356:359	e.g.	356:359	The low abundance of endogenous glycopeptides and abundant interferents (e.g., large-size proteins and heteropeptides) in complex biological matrices render the direct analysis of endogenous glycopeptides difficult.
31750645	5	29	theme	μL	1326:1327	arg1	sample					1329:1334	10 μL sample	1323:1334	10 μL sample of human saliva treated with the MCNC@COF@GSH microspheres, which is the unprecedented high efficiency in endogenous N-linked glycopeptide enrichment from human saliva	1323:1502	More excitingly, 143 endogenous N-linked glycopeptides were clearly identified from 10 μL sample of human saliva treated with the MCNC@COF@GSH microspheres, which is the unprecedented high efficiency in endogenous N-linked glycopeptide enrichment from human saliva.
31750645	4	30	with	performances	922:933	arg1	selectivity					1019:1029	high selectivity	1014:1029	high selectivity (1:5000, human immunoglobulin G (IgG) digests to bovine serum albumin digests)	1014:1108	The as-prepared MCNC@COF@GSH microspheres possessed fast magnetic responsiveness, regular porosity, large surface areas, and good hydrophilicity, resulting in remarkable performances in N-linked glycopeptide enrichment with low detection limit (0.01 fmol μL-1), high selectivity (1:5000, human immunoglobulin G (IgG) digests to bovine serum albumin digests), excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500), and reusability (at least five times).
31750645	4	30	with	performances	922:933	arg1	albumin					1173:1179	IgG digests/IgG/bovine serum albumin	1144:1179	IgG digests/IgG/bovine serum albumin (BSA)	1144:1185	The as-prepared MCNC@COF@GSH microspheres possessed fast magnetic responsiveness, regular porosity, large surface areas, and good hydrophilicity, resulting in remarkable performances in N-linked glycopeptide enrichment with low detection limit (0.01 fmol μL-1), high selectivity (1:5000, human immunoglobulin G (IgG) digests to bovine serum albumin digests), excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500), and reusability (at least five times).
31750645	4	30	with	performances	922:933	arg1	effect					1136:1141	excellent size-exclusion effect	1111:1141	excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500)	1111:1197	The as-prepared MCNC@COF@GSH microspheres possessed fast magnetic responsiveness, regular porosity, large surface areas, and good hydrophilicity, resulting in remarkable performances in N-linked glycopeptide enrichment with low detection limit (0.01 fmol μL-1), high selectivity (1:5000, human immunoglobulin G (IgG) digests to bovine serum albumin digests), excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500), and reusability (at least five times).
31750645	4	30	with	performances	922:933	arg1	times					1231:1235	at least five times	1217:1235	at least five times	1217:1235	The as-prepared MCNC@COF@GSH microspheres possessed fast magnetic responsiveness, regular porosity, large surface areas, and good hydrophilicity, resulting in remarkable performances in N-linked glycopeptide enrichment with low detection limit (0.01 fmol μL-1), high selectivity (1:5000, human immunoglobulin G (IgG) digests to bovine serum albumin digests), excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500), and reusability (at least five times).
31750645	4	30	with	performances	922:933	arg1	μL-1					1007:1010	0.01 fmol μL-1	997:1010	0.01 fmol μL-1	997:1010	The as-prepared MCNC@COF@GSH microspheres possessed fast magnetic responsiveness, regular porosity, large surface areas, and good hydrophilicity, resulting in remarkable performances in N-linked glycopeptide enrichment with low detection limit (0.01 fmol μL-1), high selectivity (1:5000, human immunoglobulin G (IgG) digests to bovine serum albumin digests), excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500), and reusability (at least five times).
31750645	4	30	with	performances	922:933	arg1	reusability					1204:1214	reusability	1204:1214	reusability (at least five times)	1204:1236	The as-prepared MCNC@COF@GSH microspheres possessed fast magnetic responsiveness, regular porosity, large surface areas, and good hydrophilicity, resulting in remarkable performances in N-linked glycopeptide enrichment with low detection limit (0.01 fmol μL-1), high selectivity (1:5000, human immunoglobulin G (IgG) digests to bovine serum albumin digests), excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500), and reusability (at least five times).
31750645	4	30	with	performances	922:933	arg1	limit					990:994	low detection limit	976:994	low detection limit (0.01 fmol μL-1)	976:1011	The as-prepared MCNC@COF@GSH microspheres possessed fast magnetic responsiveness, regular porosity, large surface areas, and good hydrophilicity, resulting in remarkable performances in N-linked glycopeptide enrichment with low detection limit (0.01 fmol μL-1), high selectivity (1:5000, human immunoglobulin G (IgG) digests to bovine serum albumin digests), excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500), and reusability (at least five times).
31750645	2	31	theme	endogenous	304:313	arg1	glycopeptides					315:327	endogenous glycopeptides	304:327	endogenous glycopeptides	304:327	The low abundance of endogenous glycopeptides and abundant interferents (e.g., large-size proteins and heteropeptides) in complex biological matrices render the direct analysis of endogenous glycopeptides difficult.
31750645	4	32	theme	G	1061:1061	arg1	digests					1069:1075	human immunoglobulin G (IgG) digests	1040:1075	human immunoglobulin G (IgG) digests to bovine serum albumin digests	1040:1107	The as-prepared MCNC@COF@GSH microspheres possessed fast magnetic responsiveness, regular porosity, large surface areas, and good hydrophilicity, resulting in remarkable performances in N-linked glycopeptide enrichment with low detection limit (0.01 fmol μL-1), high selectivity (1:5000, human immunoglobulin G (IgG) digests to bovine serum albumin digests), excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500), and reusability (at least five times).
31750645	0	33	from	Recognition	124:134	arg1	Saliva					145:150	Human Saliva	139:150	Human Saliva	139:150	Glutathione-Functionalized Magnetic Covalent Organic Framework Microspheres with Size Exclusion for Endogenous Glycopeptide Recognition in Human Saliva.
31750645	4	34	theme	@	776:776	arg1	microspheres					781:792	The as-prepared MCNC@COF@GSH microspheres	752:792	The as-prepared MCNC@COF@GSH microspheres	752:792	The as-prepared MCNC@COF@GSH microspheres possessed fast magnetic responsiveness, regular porosity, large surface areas, and good hydrophilicity, resulting in remarkable performances in N-linked glycopeptide enrichment with low detection limit (0.01 fmol μL-1), high selectivity (1:5000, human immunoglobulin G (IgG) digests to bovine serum albumin digests), excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500), and reusability (at least five times).
31750645	5	35	gly	glycopeptide	1462:1473	arg2	glycopeptide					1462:1473	endogenous N-linked glycopeptide enrichment	1442:1484	endogenous N-linked glycopeptide enrichment from human saliva	1442:1502	More excitingly, 143 endogenous N-linked glycopeptides were clearly identified from 10 μL sample of human saliva treated with the MCNC@COF@GSH microspheres, which is the unprecedented high efficiency in endogenous N-linked glycopeptide enrichment from human saliva.
31750645	0	36	theme	Endogenous	100:109	arg1	Recognition					124:134	Endogenous Glycopeptide Recognition	100:134	Endogenous Glycopeptide Recognition in Human Saliva	100:150	Glutathione-Functionalized Magnetic Covalent Organic Framework Microspheres with Size Exclusion for Endogenous Glycopeptide Recognition in Human Saliva.
31750645	6	37	theme	glycoproteome	1681:1693	arg1	analysis					1695:1702	endogenous glycoproteome analysis	1670:1702	endogenous glycoproteome analysis	1670:1702	In addition to providing a strategy for versatile functionalization of magnetic covalent organic frameworks (COFs), this study may be used to develop application of endogenous glycoproteome analysis.
31750645	4	38	theme	surface	858:864	arg1	areas					866:870	large surface areas	852:870	large surface areas	852:870	The as-prepared MCNC@COF@GSH microspheres possessed fast magnetic responsiveness, regular porosity, large surface areas, and good hydrophilicity, resulting in remarkable performances in N-linked glycopeptide enrichment with low detection limit (0.01 fmol μL-1), high selectivity (1:5000, human immunoglobulin G (IgG) digests to bovine serum albumin digests), excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500), and reusability (at least five times).
31750645	4	39	theme	human	1040:1044	arg1	G					1061:1061	human immunoglobulin G	1040:1061	human immunoglobulin G (IgG) digests to bovine serum albumin digests	1040:1107	The as-prepared MCNC@COF@GSH microspheres possessed fast magnetic responsiveness, regular porosity, large surface areas, and good hydrophilicity, resulting in remarkable performances in N-linked glycopeptide enrichment with low detection limit (0.01 fmol μL-1), high selectivity (1:5000, human immunoglobulin G (IgG) digests to bovine serum albumin digests), excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500), and reusability (at least five times).
31750645	4	39	theme	human	1040:1044	arg1	IgG					1064:1066	IgG	1064:1066	IgG	1064:1066	The as-prepared MCNC@COF@GSH microspheres possessed fast magnetic responsiveness, regular porosity, large surface areas, and good hydrophilicity, resulting in remarkable performances in N-linked glycopeptide enrichment with low detection limit (0.01 fmol μL-1), high selectivity (1:5000, human immunoglobulin G (IgG) digests to bovine serum albumin digests), excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500), and reusability (at least five times).
31750645	5	40	theme	human	1491:1495	arg1	saliva					1497:1502	human saliva	1491:1502	human saliva	1491:1502	More excitingly, 143 endogenous N-linked glycopeptides were clearly identified from 10 μL sample of human saliva treated with the MCNC@COF@GSH microspheres, which is the unprecedented high efficiency in endogenous N-linked glycopeptide enrichment from human saliva.
31750645	5	41	theme	MCNC	1369:1372	arg1	microspheres					1382:1393	the MCNC@COF@GSH microspheres	1365:1393	the MCNC@COF@GSH microspheres	1365:1393	More excitingly, 143 endogenous N-linked glycopeptides were clearly identified from 10 μL sample of human saliva treated with the MCNC@COF@GSH microspheres, which is the unprecedented high efficiency in endogenous N-linked glycopeptide enrichment from human saliva.
31750645	5	41	theme	MCNC	1369:1372	arg1	efficiency					1428:1437	the unprecedented high efficiency	1405:1437	the unprecedented high efficiency in endogenous N-linked glycopeptide enrichment from human saliva	1405:1502	More excitingly, 143 endogenous N-linked glycopeptides were clearly identified from 10 μL sample of human saliva treated with the MCNC@COF@GSH microspheres, which is the unprecedented high efficiency in endogenous N-linked glycopeptide enrichment from human saliva.
31750645	0	42	theme	Human	139:143	arg1	Saliva					145:150	Human Saliva	139:150	Human Saliva	139:150	Glutathione-Functionalized Magnetic Covalent Organic Framework Microspheres with Size Exclusion for Endogenous Glycopeptide Recognition in Human Saliva.
31750645	6	43	used	used	1639:1642	arg2	study					1626:1630	this study	1621:1630	this study	1621:1630	In addition to providing a strategy for versatile functionalization of magnetic covalent organic frameworks (COFs), this study may be used to develop application of endogenous glycoproteome analysis.
31750645	4	44	theme	albumin	1093:1099	arg1	digests					1101:1107	bovine serum albumin digests	1080:1107	bovine serum albumin digests	1080:1107	The as-prepared MCNC@COF@GSH microspheres possessed fast magnetic responsiveness, regular porosity, large surface areas, and good hydrophilicity, resulting in remarkable performances in N-linked glycopeptide enrichment with low detection limit (0.01 fmol μL-1), high selectivity (1:5000, human immunoglobulin G (IgG) digests to bovine serum albumin digests), excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500), and reusability (at least five times).
31750645	3	45	theme	@	615:615	arg1	GSH					620:622	MCNC@COF@GSH	611:622	MCNC@COF@GSH	611:622	Reported here is a novel glutathione-functionalized magnetic covalent organic framework microsphere (denoted as MCNC@COF@GSH) endowed with size-exclusion effect and strong hydrophilicity for selective and efficient enrichment of N-linked glycopeptides.
31750645	4	46	theme	regular	834:840	arg1	porosity					842:849	regular porosity	834:849	regular porosity	834:849	The as-prepared MCNC@COF@GSH microspheres possessed fast magnetic responsiveness, regular porosity, large surface areas, and good hydrophilicity, resulting in remarkable performances in N-linked glycopeptide enrichment with low detection limit (0.01 fmol μL-1), high selectivity (1:5000, human immunoglobulin G (IgG) digests to bovine serum albumin digests), excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500), and reusability (at least five times).
31750645	5	47	theme	COF	1374:1376	arg1	microspheres					1382:1393	the MCNC@COF@GSH microspheres	1365:1393	the MCNC@COF@GSH microspheres	1365:1393	More excitingly, 143 endogenous N-linked glycopeptides were clearly identified from 10 μL sample of human saliva treated with the MCNC@COF@GSH microspheres, which is the unprecedented high efficiency in endogenous N-linked glycopeptide enrichment from human saliva.
31750645	5	47	theme	COF	1374:1376	arg1	efficiency					1428:1437	the unprecedented high efficiency	1405:1437	the unprecedented high efficiency in endogenous N-linked glycopeptide enrichment from human saliva	1405:1502	More excitingly, 143 endogenous N-linked glycopeptides were clearly identified from 10 μL sample of human saliva treated with the MCNC@COF@GSH microspheres, which is the unprecedented high efficiency in endogenous N-linked glycopeptide enrichment from human saliva.
31750645	2	48	theme	direct	444:449	arg1	analysis					451:458	the direct analysis	440:458	the direct analysis of endogenous glycopeptides	440:486	The low abundance of endogenous glycopeptides and abundant interferents (e.g., large-size proteins and heteropeptides) in complex biological matrices render the direct analysis of endogenous glycopeptides difficult.
31750645	0	49	theme	Glutathione-Functionalized	0:25	arg1	Microspheres					63:74	Glutathione-Functionalized Magnetic Covalent Organic Framework Microspheres	0:74	Glutathione-Functionalized Magnetic Covalent Organic Framework Microspheres with Size Exclusion for Endogenous Glycopeptide Recognition in Human Saliva.	0:151	Glutathione-Functionalized Magnetic Covalent Organic Framework Microspheres with Size Exclusion for Endogenous Glycopeptide Recognition in Human Saliva.
31750645	6	50	theme	analysis	1695:1702	arg1	application					1655:1665	application	1655:1665	application of endogenous glycoproteome analysis	1655:1702	In addition to providing a strategy for versatile functionalization of magnetic covalent organic frameworks (COFs), this study may be used to develop application of endogenous glycoproteome analysis.
31750645	5	51	theme	saliva	1345:1350	arg1	sample					1329:1334	10 μL sample	1323:1334	10 μL sample of human saliva treated with the MCNC@COF@GSH microspheres, which is the unprecedented high efficiency in endogenous N-linked glycopeptide enrichment from human saliva	1323:1502	More excitingly, 143 endogenous N-linked glycopeptides were clearly identified from 10 μL sample of human saliva treated with the MCNC@COF@GSH microspheres, which is the unprecedented high efficiency in endogenous N-linked glycopeptide enrichment from human saliva.
31750645	3	52	theme	magnetic	551:558	arg1	microsphere					587:597	a novel glutathione-functionalized magnetic covalent organic framework microsphere	516:597	a novel glutathione-functionalized magnetic covalent organic framework microsphere (denoted as MCNC@COF@GSH)	516:623	Reported here is a novel glutathione-functionalized magnetic covalent organic framework microsphere (denoted as MCNC@COF@GSH) endowed with size-exclusion effect and strong hydrophilicity for selective and efficient enrichment of N-linked glycopeptides.
31750645	0	53	theme	Covalent	36:43	arg1	Microspheres					63:74	Glutathione-Functionalized Magnetic Covalent Organic Framework Microspheres	0:74	Glutathione-Functionalized Magnetic Covalent Organic Framework Microspheres with Size Exclusion for Endogenous Glycopeptide Recognition in Human Saliva.	0:151	Glutathione-Functionalized Magnetic Covalent Organic Framework Microspheres with Size Exclusion for Endogenous Glycopeptide Recognition in Human Saliva.
31750645	3	54	theme	COF	616:618	arg1	GSH					620:622	MCNC@COF@GSH	611:622	MCNC@COF@GSH	611:622	Reported here is a novel glutathione-functionalized magnetic covalent organic framework microsphere (denoted as MCNC@COF@GSH) endowed with size-exclusion effect and strong hydrophilicity for selective and efficient enrichment of N-linked glycopeptides.
31750645	2	55	theme	glycopeptides	474:486	arg1	analysis					451:458	the direct analysis	440:458	the direct analysis of endogenous glycopeptides	440:486	The low abundance of endogenous glycopeptides and abundant interferents (e.g., large-size proteins and heteropeptides) in complex biological matrices render the direct analysis of endogenous glycopeptides difficult.
31750645	3	56	theme	novel	518:522	arg1	microsphere					587:597	a novel glutathione-functionalized magnetic covalent organic framework microsphere	516:597	a novel glutathione-functionalized magnetic covalent organic framework microsphere (denoted as MCNC@COF@GSH)	516:623	Reported here is a novel glutathione-functionalized magnetic covalent organic framework microsphere (denoted as MCNC@COF@GSH) endowed with size-exclusion effect and strong hydrophilicity for selective and efficient enrichment of N-linked glycopeptides.
31750645	0	57	theme	Framework	53:61	arg1	Microspheres					63:74	Glutathione-Functionalized Magnetic Covalent Organic Framework Microspheres	0:74	Glutathione-Functionalized Magnetic Covalent Organic Framework Microspheres with Size Exclusion for Endogenous Glycopeptide Recognition in Human Saliva.	0:151	Glutathione-Functionalized Magnetic Covalent Organic Framework Microspheres with Size Exclusion for Endogenous Glycopeptide Recognition in Human Saliva.
31750645	4	58	theme	bovine	1080:1085	arg1	digests					1101:1107	bovine serum albumin digests	1080:1107	bovine serum albumin digests	1080:1107	The as-prepared MCNC@COF@GSH microspheres possessed fast magnetic responsiveness, regular porosity, large surface areas, and good hydrophilicity, resulting in remarkable performances in N-linked glycopeptide enrichment with low detection limit (0.01 fmol μL-1), high selectivity (1:5000, human immunoglobulin G (IgG) digests to bovine serum albumin digests), excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500), and reusability (at least five times).
31750645	3	59	theme	N-linked	728:735	arg1	glycopeptides					737:749	N-linked glycopeptides	728:749	N-linked glycopeptides	728:749	Reported here is a novel glutathione-functionalized magnetic covalent organic framework microsphere (denoted as MCNC@COF@GSH) endowed with size-exclusion effect and strong hydrophilicity for selective and efficient enrichment of N-linked glycopeptides.
31750645	4	60	theme	IgG	1144:1146	arg1	BSA					1182:1184	BSA	1182:1184	BSA	1182:1184	The as-prepared MCNC@COF@GSH microspheres possessed fast magnetic responsiveness, regular porosity, large surface areas, and good hydrophilicity, resulting in remarkable performances in N-linked glycopeptide enrichment with low detection limit (0.01 fmol μL-1), high selectivity (1:5000, human immunoglobulin G (IgG) digests to bovine serum albumin digests), excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500), and reusability (at least five times).
31750645	4	60	theme	IgG	1144:1146	arg1	albumin					1173:1179	IgG digests/IgG/bovine serum albumin	1144:1179	IgG digests/IgG/bovine serum albumin (BSA)	1144:1185	The as-prepared MCNC@COF@GSH microspheres possessed fast magnetic responsiveness, regular porosity, large surface areas, and good hydrophilicity, resulting in remarkable performances in N-linked glycopeptide enrichment with low detection limit (0.01 fmol μL-1), high selectivity (1:5000, human immunoglobulin G (IgG) digests to bovine serum albumin digests), excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500), and reusability (at least five times).
31750645	4	60	theme	IgG	1144:1146	arg1	effect					1136:1141	excellent size-exclusion effect	1111:1141	excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500)	1111:1197	The as-prepared MCNC@COF@GSH microspheres possessed fast magnetic responsiveness, regular porosity, large surface areas, and good hydrophilicity, resulting in remarkable performances in N-linked glycopeptide enrichment with low detection limit (0.01 fmol μL-1), high selectivity (1:5000, human immunoglobulin G (IgG) digests to bovine serum albumin digests), excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500), and reusability (at least five times).
31750645	4	61	theme	good	877:880	arg1	hydrophilicity					882:895	good hydrophilicity	877:895	good hydrophilicity	877:895	The as-prepared MCNC@COF@GSH microspheres possessed fast magnetic responsiveness, regular porosity, large surface areas, and good hydrophilicity, resulting in remarkable performances in N-linked glycopeptide enrichment with low detection limit (0.01 fmol μL-1), high selectivity (1:5000, human immunoglobulin G (IgG) digests to bovine serum albumin digests), excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500), and reusability (at least five times).
31750645	5	62	theme	unprecedented	1409:1421	arg1	microspheres					1382:1393	the MCNC@COF@GSH microspheres	1365:1393	the MCNC@COF@GSH microspheres	1365:1393	More excitingly, 143 endogenous N-linked glycopeptides were clearly identified from 10 μL sample of human saliva treated with the MCNC@COF@GSH microspheres, which is the unprecedented high efficiency in endogenous N-linked glycopeptide enrichment from human saliva.
31750645	5	62	theme	unprecedented	1409:1421	arg1	efficiency					1428:1437	the unprecedented high efficiency	1405:1437	the unprecedented high efficiency in endogenous N-linked glycopeptide enrichment from human saliva	1405:1502	More excitingly, 143 endogenous N-linked glycopeptides were clearly identified from 10 μL sample of human saliva treated with the MCNC@COF@GSH microspheres, which is the unprecedented high efficiency in endogenous N-linked glycopeptide enrichment from human saliva.
31750645	3	63	theme	organic	569:575	arg1	microsphere					587:597	a novel glutathione-functionalized magnetic covalent organic framework microsphere	516:597	a novel glutathione-functionalized magnetic covalent organic framework microsphere (denoted as MCNC@COF@GSH)	516:623	Reported here is a novel glutathione-functionalized magnetic covalent organic framework microsphere (denoted as MCNC@COF@GSH) endowed with size-exclusion effect and strong hydrophilicity for selective and efficient enrichment of N-linked glycopeptides.
31750645	5	64	from	efficiency	1428:1437	arg1	enrichment					1475:1484	endogenous N-linked glycopeptide enrichment	1442:1484	endogenous N-linked glycopeptide enrichment from human saliva	1442:1502	More excitingly, 143 endogenous N-linked glycopeptides were clearly identified from 10 μL sample of human saliva treated with the MCNC@COF@GSH microspheres, which is the unprecedented high efficiency in endogenous N-linked glycopeptide enrichment from human saliva.
31750645	5	64	from	efficiency	1428:1437	arg1	saliva					1497:1502	human saliva	1491:1502	human saliva	1491:1502	More excitingly, 143 endogenous N-linked glycopeptides were clearly identified from 10 μL sample of human saliva treated with the MCNC@COF@GSH microspheres, which is the unprecedented high efficiency in endogenous N-linked glycopeptide enrichment from human saliva.
31750645	4	65	theme	serum	1167:1171	arg1	BSA					1182:1184	BSA	1182:1184	BSA	1182:1184	The as-prepared MCNC@COF@GSH microspheres possessed fast magnetic responsiveness, regular porosity, large surface areas, and good hydrophilicity, resulting in remarkable performances in N-linked glycopeptide enrichment with low detection limit (0.01 fmol μL-1), high selectivity (1:5000, human immunoglobulin G (IgG) digests to bovine serum albumin digests), excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500), and reusability (at least five times).
31750645	4	65	theme	serum	1167:1171	arg1	albumin					1173:1179	IgG digests/IgG/bovine serum albumin	1144:1179	IgG digests/IgG/bovine serum albumin (BSA)	1144:1185	The as-prepared MCNC@COF@GSH microspheres possessed fast magnetic responsiveness, regular porosity, large surface areas, and good hydrophilicity, resulting in remarkable performances in N-linked glycopeptide enrichment with low detection limit (0.01 fmol μL-1), high selectivity (1:5000, human immunoglobulin G (IgG) digests to bovine serum albumin digests), excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500), and reusability (at least five times).
31750645	4	65	theme	serum	1167:1171	arg1	effect					1136:1141	excellent size-exclusion effect	1111:1141	excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500)	1111:1197	The as-prepared MCNC@COF@GSH microspheres possessed fast magnetic responsiveness, regular porosity, large surface areas, and good hydrophilicity, resulting in remarkable performances in N-linked glycopeptide enrichment with low detection limit (0.01 fmol μL-1), high selectivity (1:5000, human immunoglobulin G (IgG) digests to bovine serum albumin digests), excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500), and reusability (at least five times).
31750645	2	66	theme	endogenous	463:472	arg1	glycopeptides					474:486	endogenous glycopeptides	463:486	endogenous glycopeptides	463:486	The low abundance of endogenous glycopeptides and abundant interferents (e.g., large-size proteins and heteropeptides) in complex biological matrices render the direct analysis of endogenous glycopeptides difficult.
31750645	5	67	theme	GSH	1378:1380	arg1	microspheres					1382:1393	the MCNC@COF@GSH microspheres	1365:1393	the MCNC@COF@GSH microspheres	1365:1393	More excitingly, 143 endogenous N-linked glycopeptides were clearly identified from 10 μL sample of human saliva treated with the MCNC@COF@GSH microspheres, which is the unprecedented high efficiency in endogenous N-linked glycopeptide enrichment from human saliva.
31750645	5	67	theme	GSH	1378:1380	arg1	efficiency					1428:1437	the unprecedented high efficiency	1405:1437	the unprecedented high efficiency in endogenous N-linked glycopeptide enrichment from human saliva	1405:1502	More excitingly, 143 endogenous N-linked glycopeptides were clearly identified from 10 μL sample of human saliva treated with the MCNC@COF@GSH microspheres, which is the unprecedented high efficiency in endogenous N-linked glycopeptide enrichment from human saliva.
31750645	3	68	theme	MCNC	611:614	arg1	GSH					620:622	MCNC@COF@GSH	611:622	MCNC@COF@GSH	611:622	Reported here is a novel glutathione-functionalized magnetic covalent organic framework microsphere (denoted as MCNC@COF@GSH) endowed with size-exclusion effect and strong hydrophilicity for selective and efficient enrichment of N-linked glycopeptides.
31750645	5	69	theme	N-linked	1271:1278	arg1	glycopeptides					1280:1292	143 endogenous N-linked glycopeptides	1256:1292	143 endogenous N-linked glycopeptides	1256:1292	More excitingly, 143 endogenous N-linked glycopeptides were clearly identified from 10 μL sample of human saliva treated with the MCNC@COF@GSH microspheres, which is the unprecedented high efficiency in endogenous N-linked glycopeptide enrichment from human saliva.
31750645	4	70	theme	excellent	1111:1119	arg1	albumin					1173:1179	IgG digests/IgG/bovine serum albumin	1144:1179	IgG digests/IgG/bovine serum albumin (BSA)	1144:1185	The as-prepared MCNC@COF@GSH microspheres possessed fast magnetic responsiveness, regular porosity, large surface areas, and good hydrophilicity, resulting in remarkable performances in N-linked glycopeptide enrichment with low detection limit (0.01 fmol μL-1), high selectivity (1:5000, human immunoglobulin G (IgG) digests to bovine serum albumin digests), excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500), and reusability (at least five times).
31750645	4	70	theme	excellent	1111:1119	arg1	effect					1136:1141	excellent size-exclusion effect	1111:1141	excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500)	1111:1197	The as-prepared MCNC@COF@GSH microspheres possessed fast magnetic responsiveness, regular porosity, large surface areas, and good hydrophilicity, resulting in remarkable performances in N-linked glycopeptide enrichment with low detection limit (0.01 fmol μL-1), high selectivity (1:5000, human immunoglobulin G (IgG) digests to bovine serum albumin digests), excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500), and reusability (at least five times).
31750645	2	71	theme	biological	413:422	arg1	matrices					424:431	complex biological matrices	405:431	complex biological matrices	405:431	The low abundance of endogenous glycopeptides and abundant interferents (e.g., large-size proteins and heteropeptides) in complex biological matrices render the direct analysis of endogenous glycopeptides difficult.
31750645	3	72	gly	glycopeptides	737:749	arg2	glycopeptides					737:749	N-linked glycopeptides	728:749	N-linked glycopeptides	728:749	Reported here is a novel glutathione-functionalized magnetic covalent organic framework microsphere (denoted as MCNC@COF@GSH) endowed with size-exclusion effect and strong hydrophilicity for selective and efficient enrichment of N-linked glycopeptides.
31750645	5	73	theme	glycopeptide	1462:1473	arg1	enrichment					1475:1484	endogenous N-linked glycopeptide enrichment	1442:1484	endogenous N-linked glycopeptide enrichment from human saliva	1442:1502	More excitingly, 143 endogenous N-linked glycopeptides were clearly identified from 10 μL sample of human saliva treated with the MCNC@COF@GSH microspheres, which is the unprecedented high efficiency in endogenous N-linked glycopeptide enrichment from human saliva.
31750645	4	74	theme	detection	980:988	arg1	μL-1					1007:1010	0.01 fmol μL-1	997:1010	0.01 fmol μL-1	997:1010	The as-prepared MCNC@COF@GSH microspheres possessed fast magnetic responsiveness, regular porosity, large surface areas, and good hydrophilicity, resulting in remarkable performances in N-linked glycopeptide enrichment with low detection limit (0.01 fmol μL-1), high selectivity (1:5000, human immunoglobulin G (IgG) digests to bovine serum albumin digests), excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500), and reusability (at least five times).
31750645	4	74	theme	detection	980:988	arg1	limit					990:994	low detection limit	976:994	low detection limit (0.01 fmol μL-1)	976:1011	The as-prepared MCNC@COF@GSH microspheres possessed fast magnetic responsiveness, regular porosity, large surface areas, and good hydrophilicity, resulting in remarkable performances in N-linked glycopeptide enrichment with low detection limit (0.01 fmol μL-1), high selectivity (1:5000, human immunoglobulin G (IgG) digests to bovine serum albumin digests), excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500), and reusability (at least five times).
31750645	2	75	theme	abundant	333:340	arg1	interferents					342:353	abundant interferents	333:353	abundant interferents (e.g., large-size proteins and heteropeptides)	333:400	The low abundance of endogenous glycopeptides and abundant interferents (e.g., large-size proteins and heteropeptides) in complex biological matrices render the direct analysis of endogenous glycopeptides difficult.
31750645	6	76	theme	covalent	1585:1592	arg1	COFs					1614:1617	COFs	1614:1617	COFs	1614:1617	In addition to providing a strategy for versatile functionalization of magnetic covalent organic frameworks (COFs), this study may be used to develop application of endogenous glycoproteome analysis.
31750645	6	76	theme	covalent	1585:1592	arg1	frameworks					1602:1611	magnetic covalent organic frameworks	1576:1611	magnetic covalent organic frameworks (COFs)	1576:1618	In addition to providing a strategy for versatile functionalization of magnetic covalent organic frameworks (COFs), this study may be used to develop application of endogenous glycoproteome analysis.
31750645	5	77	link	N-linked	1271:1278	arg1	glycopeptides					1280:1292	143 endogenous N-linked glycopeptides	1256:1292	143 endogenous N-linked glycopeptides	1256:1292	More excitingly, 143 endogenous N-linked glycopeptides were clearly identified from 10 μL sample of human saliva treated with the MCNC@COF@GSH microspheres, which is the unprecedented high efficiency in endogenous N-linked glycopeptide enrichment from human saliva.
31750645	3	78	theme	size-exclusion	638:651	arg1	effect					653:658	size-exclusion effect	638:658	size-exclusion effect	638:658	Reported here is a novel glutathione-functionalized magnetic covalent organic framework microsphere (denoted as MCNC@COF@GSH) endowed with size-exclusion effect and strong hydrophilicity for selective and efficient enrichment of N-linked glycopeptides.
31750645	4	79	theme	glycopeptide	947:958	arg1	enrichment					960:969	N-linked glycopeptide enrichment	938:969	N-linked glycopeptide enrichment	938:969	The as-prepared MCNC@COF@GSH microspheres possessed fast magnetic responsiveness, regular porosity, large surface areas, and good hydrophilicity, resulting in remarkable performances in N-linked glycopeptide enrichment with low detection limit (0.01 fmol μL-1), high selectivity (1:5000, human immunoglobulin G (IgG) digests to bovine serum albumin digests), excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500), and reusability (at least five times).
31750645	4	80	theme	MCNC	768:771	arg1	microspheres					781:792	The as-prepared MCNC@COF@GSH microspheres	752:792	The as-prepared MCNC@COF@GSH microspheres	752:792	The as-prepared MCNC@COF@GSH microspheres possessed fast magnetic responsiveness, regular porosity, large surface areas, and good hydrophilicity, resulting in remarkable performances in N-linked glycopeptide enrichment with low detection limit (0.01 fmol μL-1), high selectivity (1:5000, human immunoglobulin G (IgG) digests to bovine serum albumin digests), excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500), and reusability (at least five times).
31750645	5	81	theme	endogenous	1442:1451	arg1	enrichment					1475:1484	endogenous N-linked glycopeptide enrichment	1442:1484	endogenous N-linked glycopeptide enrichment from human saliva	1442:1502	More excitingly, 143 endogenous N-linked glycopeptides were clearly identified from 10 μL sample of human saliva treated with the MCNC@COF@GSH microspheres, which is the unprecedented high efficiency in endogenous N-linked glycopeptide enrichment from human saliva.
31750645	6	82	theme	versatile	1545:1553	arg1	functionalization					1555:1571	versatile functionalization	1545:1571	versatile functionalization of magnetic covalent organic frameworks (COFs)	1545:1618	In addition to providing a strategy for versatile functionalization of magnetic covalent organic frameworks (COFs), this study may be used to develop application of endogenous glycoproteome analysis.
31750645	1	83	theme	multifarious	228:239	arg1	processes					272:280	multifarious pathological and physiological processes	228:280	multifarious pathological and physiological processes	228:280	Endogenous glycopeptides have been confirmed to play a significant role in multifarious pathological and physiological processes.
31750645	4	84	theme	GSH	777:779	arg1	microspheres					781:792	The as-prepared MCNC@COF@GSH microspheres	752:792	The as-prepared MCNC@COF@GSH microspheres	752:792	The as-prepared MCNC@COF@GSH microspheres possessed fast magnetic responsiveness, regular porosity, large surface areas, and good hydrophilicity, resulting in remarkable performances in N-linked glycopeptide enrichment with low detection limit (0.01 fmol μL-1), high selectivity (1:5000, human immunoglobulin G (IgG) digests to bovine serum albumin digests), excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500), and reusability (at least five times).
31750645	4	85	dep	1:5000	1032:1037	arg1	digests					1069:1075	human immunoglobulin G (IgG) digests	1040:1075	human immunoglobulin G (IgG) digests to bovine serum albumin digests	1040:1107	The as-prepared MCNC@COF@GSH microspheres possessed fast magnetic responsiveness, regular porosity, large surface areas, and good hydrophilicity, resulting in remarkable performances in N-linked glycopeptide enrichment with low detection limit (0.01 fmol μL-1), high selectivity (1:5000, human immunoglobulin G (IgG) digests to bovine serum albumin digests), excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500), and reusability (at least five times).
31750645	2	86	theme	low	287:289	arg1	abundance					291:299	The low abundance	283:299	The low abundance of endogenous glycopeptides and abundant interferents (e.g., large-size proteins and heteropeptides) in complex biological matrices	283:431	The low abundance of endogenous glycopeptides and abundant interferents (e.g., large-size proteins and heteropeptides) in complex biological matrices render the direct analysis of endogenous glycopeptides difficult.
31750645	4	87	gly	glycopeptide	947:958	arg2	glycopeptide					947:958	N-linked glycopeptide enrichment	938:969	N-linked glycopeptide enrichment	938:969	The as-prepared MCNC@COF@GSH microspheres possessed fast magnetic responsiveness, regular porosity, large surface areas, and good hydrophilicity, resulting in remarkable performances in N-linked glycopeptide enrichment with low detection limit (0.01 fmol μL-1), high selectivity (1:5000, human immunoglobulin G (IgG) digests to bovine serum albumin digests), excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500), and reusability (at least five times).
31750645	4	88	theme	high	1014:1017	arg1	selectivity					1019:1029	high selectivity	1014:1029	high selectivity (1:5000, human immunoglobulin G (IgG) digests to bovine serum albumin digests)	1014:1108	The as-prepared MCNC@COF@GSH microspheres possessed fast magnetic responsiveness, regular porosity, large surface areas, and good hydrophilicity, resulting in remarkable performances in N-linked glycopeptide enrichment with low detection limit (0.01 fmol μL-1), high selectivity (1:5000, human immunoglobulin G (IgG) digests to bovine serum albumin digests), excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500), and reusability (at least five times).
31750645	4	89	link	N-linked	938:945	arg1	enrichment					960:969	N-linked glycopeptide enrichment	938:969	N-linked glycopeptide enrichment	938:969	The as-prepared MCNC@COF@GSH microspheres possessed fast magnetic responsiveness, regular porosity, large surface areas, and good hydrophilicity, resulting in remarkable performances in N-linked glycopeptide enrichment with low detection limit (0.01 fmol μL-1), high selectivity (1:5000, human immunoglobulin G (IgG) digests to bovine serum albumin digests), excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500), and reusability (at least five times).
31750645	3	90	theme	efficient	704:712	arg1	enrichment					714:723	selective and efficient enrichment	690:723	selective and efficient enrichment of N-linked glycopeptides	690:749	Reported here is a novel glutathione-functionalized magnetic covalent organic framework microsphere (denoted as MCNC@COF@GSH) endowed with size-exclusion effect and strong hydrophilicity for selective and efficient enrichment of N-linked glycopeptides.
31750645	4	91	dep	albumin	1173:1179	arg1	1:500:500					1188:1196	1:500:500	1188:1196	1:500:500	1188:1196	The as-prepared MCNC@COF@GSH microspheres possessed fast magnetic responsiveness, regular porosity, large surface areas, and good hydrophilicity, resulting in remarkable performances in N-linked glycopeptide enrichment with low detection limit (0.01 fmol μL-1), high selectivity (1:5000, human immunoglobulin G (IgG) digests to bovine serum albumin digests), excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500), and reusability (at least five times).
31750645	4	92	theme	fmol	1002:1005	arg1	μL-1					1007:1010	0.01 fmol μL-1	997:1010	0.01 fmol μL-1	997:1010	The as-prepared MCNC@COF@GSH microspheres possessed fast magnetic responsiveness, regular porosity, large surface areas, and good hydrophilicity, resulting in remarkable performances in N-linked glycopeptide enrichment with low detection limit (0.01 fmol μL-1), high selectivity (1:5000, human immunoglobulin G (IgG) digests to bovine serum albumin digests), excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500), and reusability (at least five times).
31750645	4	92	theme	fmol	1002:1005	arg1	limit					990:994	low detection limit	976:994	low detection limit (0.01 fmol μL-1)	976:1011	The as-prepared MCNC@COF@GSH microspheres possessed fast magnetic responsiveness, regular porosity, large surface areas, and good hydrophilicity, resulting in remarkable performances in N-linked glycopeptide enrichment with low detection limit (0.01 fmol μL-1), high selectivity (1:5000, human immunoglobulin G (IgG) digests to bovine serum albumin digests), excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500), and reusability (at least five times).
31750645	4	93	theme	magnetic	809:816	arg1	responsiveness					818:831	fast magnetic responsiveness	804:831	fast magnetic responsiveness	804:831	The as-prepared MCNC@COF@GSH microspheres possessed fast magnetic responsiveness, regular porosity, large surface areas, and good hydrophilicity, resulting in remarkable performances in N-linked glycopeptide enrichment with low detection limit (0.01 fmol μL-1), high selectivity (1:5000, human immunoglobulin G (IgG) digests to bovine serum albumin digests), excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500), and reusability (at least five times).
31750645	3	94	theme	selective	690:698	arg1	enrichment					714:723	selective and efficient enrichment	690:723	selective and efficient enrichment of N-linked glycopeptides	690:749	Reported here is a novel glutathione-functionalized magnetic covalent organic framework microsphere (denoted as MCNC@COF@GSH) endowed with size-exclusion effect and strong hydrophilicity for selective and efficient enrichment of N-linked glycopeptides.
31750645	4	95	theme	immunoglobulin	1046:1059	arg1	G					1061:1061	human immunoglobulin G	1040:1061	human immunoglobulin G (IgG) digests to bovine serum albumin digests	1040:1107	The as-prepared MCNC@COF@GSH microspheres possessed fast magnetic responsiveness, regular porosity, large surface areas, and good hydrophilicity, resulting in remarkable performances in N-linked glycopeptide enrichment with low detection limit (0.01 fmol μL-1), high selectivity (1:5000, human immunoglobulin G (IgG) digests to bovine serum albumin digests), excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500), and reusability (at least five times).
31750645	4	95	theme	immunoglobulin	1046:1059	arg1	IgG					1064:1066	IgG	1064:1066	IgG	1064:1066	The as-prepared MCNC@COF@GSH microspheres possessed fast magnetic responsiveness, regular porosity, large surface areas, and good hydrophilicity, resulting in remarkable performances in N-linked glycopeptide enrichment with low detection limit (0.01 fmol μL-1), high selectivity (1:5000, human immunoglobulin G (IgG) digests to bovine serum albumin digests), excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500), and reusability (at least five times).
31750645	4	96	theme	COF	773:775	arg1	microspheres					781:792	The as-prepared MCNC@COF@GSH microspheres	752:792	The as-prepared MCNC@COF@GSH microspheres	752:792	The as-prepared MCNC@COF@GSH microspheres possessed fast magnetic responsiveness, regular porosity, large surface areas, and good hydrophilicity, resulting in remarkable performances in N-linked glycopeptide enrichment with low detection limit (0.01 fmol μL-1), high selectivity (1:5000, human immunoglobulin G (IgG) digests to bovine serum albumin digests), excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500), and reusability (at least five times).
31750645	0	97	theme	Glycopeptide	111:122	arg1	Recognition					124:134	Endogenous Glycopeptide Recognition	100:134	Endogenous Glycopeptide Recognition in Human Saliva	100:150	Glutathione-Functionalized Magnetic Covalent Organic Framework Microspheres with Size Exclusion for Endogenous Glycopeptide Recognition in Human Saliva.
31750645	6	98	theme	frameworks	1602:1611	arg1	functionalization					1555:1571	versatile functionalization	1545:1571	versatile functionalization of magnetic covalent organic frameworks (COFs)	1545:1618	In addition to providing a strategy for versatile functionalization of magnetic covalent organic frameworks (COFs), this study may be used to develop application of endogenous glycoproteome analysis.
31750645	2	99	dep	interferents	342:353	arg1	heteropeptides					386:399	heteropeptides	386:399	heteropeptides	386:399	The low abundance of endogenous glycopeptides and abundant interferents (e.g., large-size proteins and heteropeptides) in complex biological matrices render the direct analysis of endogenous glycopeptides difficult.
31750645	2	99	dep	interferents	342:353	arg1	proteins					373:380	large-size proteins	362:380	large-size proteins	362:380	The low abundance of endogenous glycopeptides and abundant interferents (e.g., large-size proteins and heteropeptides) in complex biological matrices render the direct analysis of endogenous glycopeptides difficult.
31750645	4	100	dep	selectivity	1019:1029	arg1	1:5000					1032:1037	1:5000	1032:1037	1:5000	1032:1037	The as-prepared MCNC@COF@GSH microspheres possessed fast magnetic responsiveness, regular porosity, large surface areas, and good hydrophilicity, resulting in remarkable performances in N-linked glycopeptide enrichment with low detection limit (0.01 fmol μL-1), high selectivity (1:5000, human immunoglobulin G (IgG) digests to bovine serum albumin digests), excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500), and reusability (at least five times).
31750645	6	101	theme	endogenous	1670:1679	arg1	analysis					1695:1702	endogenous glycoproteome analysis	1670:1702	endogenous glycoproteome analysis	1670:1702	In addition to providing a strategy for versatile functionalization of magnetic covalent organic frameworks (COFs), this study may be used to develop application of endogenous glycoproteome analysis.
31750645	2	102	gly	glycopeptides	474:486	arg2	glycopeptides					474:486	endogenous glycopeptides	463:486	endogenous glycopeptides	463:486	The low abundance of endogenous glycopeptides and abundant interferents (e.g., large-size proteins and heteropeptides) in complex biological matrices render the direct analysis of endogenous glycopeptides difficult.
31750645	5	103	gly	glycopeptides	1280:1292	arg2	glycopeptides					1280:1292	143 endogenous N-linked glycopeptides	1256:1292	143 endogenous N-linked glycopeptides	1256:1292	More excitingly, 143 endogenous N-linked glycopeptides were clearly identified from 10 μL sample of human saliva treated with the MCNC@COF@GSH microspheres, which is the unprecedented high efficiency in endogenous N-linked glycopeptide enrichment from human saliva.
31750645	4	104	theme	large	852:856	arg1	areas					866:870	large surface areas	852:870	large surface areas	852:870	The as-prepared MCNC@COF@GSH microspheres possessed fast magnetic responsiveness, regular porosity, large surface areas, and good hydrophilicity, resulting in remarkable performances in N-linked glycopeptide enrichment with low detection limit (0.01 fmol μL-1), high selectivity (1:5000, human immunoglobulin G (IgG) digests to bovine serum albumin digests), excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500), and reusability (at least five times).
31750645	3	105	dep	microsphere	587:597	arg1	denoted					600:606	denoted	600:606	denoted as MCNC@COF@GSH	600:622	Reported here is a novel glutathione-functionalized magnetic covalent organic framework microsphere (denoted as MCNC@COF@GSH) endowed with size-exclusion effect and strong hydrophilicity for selective and efficient enrichment of N-linked glycopeptides.
31750645	5	106	theme	@	1373:1373	arg1	microspheres					1382:1393	the MCNC@COF@GSH microspheres	1365:1393	the MCNC@COF@GSH microspheres	1365:1393	More excitingly, 143 endogenous N-linked glycopeptides were clearly identified from 10 μL sample of human saliva treated with the MCNC@COF@GSH microspheres, which is the unprecedented high efficiency in endogenous N-linked glycopeptide enrichment from human saliva.
31750645	5	106	theme	@	1373:1373	arg1	efficiency					1428:1437	the unprecedented high efficiency	1405:1437	the unprecedented high efficiency in endogenous N-linked glycopeptide enrichment from human saliva	1405:1502	More excitingly, 143 endogenous N-linked glycopeptides were clearly identified from 10 μL sample of human saliva treated with the MCNC@COF@GSH microspheres, which is the unprecedented high efficiency in endogenous N-linked glycopeptide enrichment from human saliva.
31750645	0	107	theme	Magnetic	27:34	arg1	Microspheres					63:74	Glutathione-Functionalized Magnetic Covalent Organic Framework Microspheres	0:74	Glutathione-Functionalized Magnetic Covalent Organic Framework Microspheres with Size Exclusion for Endogenous Glycopeptide Recognition in Human Saliva.	0:151	Glutathione-Functionalized Magnetic Covalent Organic Framework Microspheres with Size Exclusion for Endogenous Glycopeptide Recognition in Human Saliva.
31750645	4	108	theme	serum	1087:1091	arg1	digests					1101:1107	bovine serum albumin digests	1080:1107	bovine serum albumin digests	1080:1107	The as-prepared MCNC@COF@GSH microspheres possessed fast magnetic responsiveness, regular porosity, large surface areas, and good hydrophilicity, resulting in remarkable performances in N-linked glycopeptide enrichment with low detection limit (0.01 fmol μL-1), high selectivity (1:5000, human immunoglobulin G (IgG) digests to bovine serum albumin digests), excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500), and reusability (at least five times).
31750645	5	109	theme	human	1339:1343	arg1	saliva					1345:1350	human saliva	1339:1350	human saliva treated with the MCNC@COF@GSH microspheres, which is the unprecedented high efficiency in endogenous N-linked glycopeptide enrichment from human saliva	1339:1502	More excitingly, 143 endogenous N-linked glycopeptides were clearly identified from 10 μL sample of human saliva treated with the MCNC@COF@GSH microspheres, which is the unprecedented high efficiency in endogenous N-linked glycopeptide enrichment from human saliva.
31750645	0	110	theme	Organic	45:51	arg1	Microspheres					63:74	Glutathione-Functionalized Magnetic Covalent Organic Framework Microspheres	0:74	Glutathione-Functionalized Magnetic Covalent Organic Framework Microspheres with Size Exclusion for Endogenous Glycopeptide Recognition in Human Saliva.	0:151	Glutathione-Functionalized Magnetic Covalent Organic Framework Microspheres with Size Exclusion for Endogenous Glycopeptide Recognition in Human Saliva.
31750645	2	111	from	abundance	291:299	arg1	matrices					424:431	complex biological matrices	405:431	complex biological matrices	405:431	The low abundance of endogenous glycopeptides and abundant interferents (e.g., large-size proteins and heteropeptides) in complex biological matrices render the direct analysis of endogenous glycopeptides difficult.
31750645	3	112	theme	glycopeptides	737:749	arg1	enrichment					714:723	selective and efficient enrichment	690:723	selective and efficient enrichment of N-linked glycopeptides	690:749	Reported here is a novel glutathione-functionalized magnetic covalent organic framework microsphere (denoted as MCNC@COF@GSH) endowed with size-exclusion effect and strong hydrophilicity for selective and efficient enrichment of N-linked glycopeptides.
31750645	3	113	theme	covalent	560:567	arg1	microsphere					587:597	a novel glutathione-functionalized magnetic covalent organic framework microsphere	516:597	a novel glutathione-functionalized magnetic covalent organic framework microsphere (denoted as MCNC@COF@GSH)	516:623	Reported here is a novel glutathione-functionalized magnetic covalent organic framework microsphere (denoted as MCNC@COF@GSH) endowed with size-exclusion effect and strong hydrophilicity for selective and efficient enrichment of N-linked glycopeptides.
31750645	3	114	theme	glutathione-functionalized	524:549	arg1	microsphere					587:597	a novel glutathione-functionalized magnetic covalent organic framework microsphere	516:597	a novel glutathione-functionalized magnetic covalent organic framework microsphere (denoted as MCNC@COF@GSH)	516:623	Reported here is a novel glutathione-functionalized magnetic covalent organic framework microsphere (denoted as MCNC@COF@GSH) endowed with size-exclusion effect and strong hydrophilicity for selective and efficient enrichment of N-linked glycopeptides.
31750645	0	115	theme	Size	81:84	arg1	Exclusion					86:94	Size Exclusion	81:94	Size Exclusion	81:94	Glutathione-Functionalized Magnetic Covalent Organic Framework Microspheres with Size Exclusion for Endogenous Glycopeptide Recognition in Human Saliva.
31750645	4	116	theme	remarkable	911:920	arg1	performances					922:933	remarkable performances	911:933	remarkable performances in N-linked glycopeptide enrichment with low detection limit (0.01 fmol μL-1), high selectivity (1:5000, human immunoglobulin G (IgG) digests to bovine serum albumin digests), excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500), and reusability (at least five times)	911:1236	The as-prepared MCNC@COF@GSH microspheres possessed fast magnetic responsiveness, regular porosity, large surface areas, and good hydrophilicity, resulting in remarkable performances in N-linked glycopeptide enrichment with low detection limit (0.01 fmol μL-1), high selectivity (1:5000, human immunoglobulin G (IgG) digests to bovine serum albumin digests), excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500), and reusability (at least five times).
31750645	5	117	theme	@	1377:1377	arg1	microspheres					1382:1393	the MCNC@COF@GSH microspheres	1365:1393	the MCNC@COF@GSH microspheres	1365:1393	More excitingly, 143 endogenous N-linked glycopeptides were clearly identified from 10 μL sample of human saliva treated with the MCNC@COF@GSH microspheres, which is the unprecedented high efficiency in endogenous N-linked glycopeptide enrichment from human saliva.
31750645	5	117	theme	@	1377:1377	arg1	efficiency					1428:1437	the unprecedented high efficiency	1405:1437	the unprecedented high efficiency in endogenous N-linked glycopeptide enrichment from human saliva	1405:1502	More excitingly, 143 endogenous N-linked glycopeptides were clearly identified from 10 μL sample of human saliva treated with the MCNC@COF@GSH microspheres, which is the unprecedented high efficiency in endogenous N-linked glycopeptide enrichment from human saliva.
31750645	3	118	theme	framework	577:585	arg1	microsphere					587:597	a novel glutathione-functionalized magnetic covalent organic framework microsphere	516:597	a novel glutathione-functionalized magnetic covalent organic framework microsphere (denoted as MCNC@COF@GSH)	516:623	Reported here is a novel glutathione-functionalized magnetic covalent organic framework microsphere (denoted as MCNC@COF@GSH) endowed with size-exclusion effect and strong hydrophilicity for selective and efficient enrichment of N-linked glycopeptides.
31750645	5	119	theme	endogenous	1260:1269	arg1	glycopeptides					1280:1292	143 endogenous N-linked glycopeptides	1256:1292	143 endogenous N-linked glycopeptides	1256:1292	More excitingly, 143 endogenous N-linked glycopeptides were clearly identified from 10 μL sample of human saliva treated with the MCNC@COF@GSH microspheres, which is the unprecedented high efficiency in endogenous N-linked glycopeptide enrichment from human saliva.
31750645	4	120	theme	digests/IgG/bovine	1148:1165	arg1	BSA					1182:1184	BSA	1182:1184	BSA	1182:1184	The as-prepared MCNC@COF@GSH microspheres possessed fast magnetic responsiveness, regular porosity, large surface areas, and good hydrophilicity, resulting in remarkable performances in N-linked glycopeptide enrichment with low detection limit (0.01 fmol μL-1), high selectivity (1:5000, human immunoglobulin G (IgG) digests to bovine serum albumin digests), excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500), and reusability (at least five times).
31750645	4	120	theme	digests/IgG/bovine	1148:1165	arg1	albumin					1173:1179	IgG digests/IgG/bovine serum albumin	1144:1179	IgG digests/IgG/bovine serum albumin (BSA)	1144:1185	The as-prepared MCNC@COF@GSH microspheres possessed fast magnetic responsiveness, regular porosity, large surface areas, and good hydrophilicity, resulting in remarkable performances in N-linked glycopeptide enrichment with low detection limit (0.01 fmol μL-1), high selectivity (1:5000, human immunoglobulin G (IgG) digests to bovine serum albumin digests), excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500), and reusability (at least five times).
31750645	4	120	theme	digests/IgG/bovine	1148:1165	arg1	effect					1136:1141	excellent size-exclusion effect	1111:1141	excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500)	1111:1197	The as-prepared MCNC@COF@GSH microspheres possessed fast magnetic responsiveness, regular porosity, large surface areas, and good hydrophilicity, resulting in remarkable performances in N-linked glycopeptide enrichment with low detection limit (0.01 fmol μL-1), high selectivity (1:5000, human immunoglobulin G (IgG) digests to bovine serum albumin digests), excellent size-exclusion effect (IgG digests/IgG/bovine serum albumin (BSA), 1:500:500), and reusability (at least five times).
31750645	0	121	with	Microspheres	63:74	arg1	Exclusion					86:94	Size Exclusion	81:94	Size Exclusion	81:94	Glutathione-Functionalized Magnetic Covalent Organic Framework Microspheres with Size Exclusion for Endogenous Glycopeptide Recognition in Human Saliva.
31750645	3	122	with	endowed	625:631	arg1	hydrophilicity					671:684	strong hydrophilicity	664:684	strong hydrophilicity	664:684	Reported here is a novel glutathione-functionalized magnetic covalent organic framework microsphere (denoted as MCNC@COF@GSH) endowed with size-exclusion effect and strong hydrophilicity for selective and efficient enrichment of N-linked glycopeptides.
31750645	3	122	with	endowed	625:631	arg1	effect					653:658	size-exclusion effect	638:658	size-exclusion effect	638:658	Reported here is a novel glutathione-functionalized magnetic covalent organic framework microsphere (denoted as MCNC@COF@GSH) endowed with size-exclusion effect and strong hydrophilicity for selective and efficient enrichment of N-linked glycopeptides.
31004166	8	0	theme	gut	978:980	arg1	analysis					993:1000	gut microbiota analysis	978:1000	gut microbiota analysis	978:1000	Fecal samples were collected for gut microbiota analysis.
31004166	10	1	theme	LFD	1391:1393	arg1	P < 0.05					1408:1415	P < 0.05	1408:1415	P < 0.05	1408:1415	The HFD group had 28%-48% lower mRNA expression of both Tjp1 and Ocln in the ileum and colon compared with levels in LFD or HSB mice (P < 0.05), whereas there was no difference in expression levels between LFD and HSB mice.
31004166	10	1	theme	LFD	1391:1393	arg1	mice					1402:1405	LFD or HSB mice	1391:1405	mice	1402:1405	The HFD group had 28%-48% lower mRNA expression of both Tjp1 and Ocln in the ileum and colon compared with levels in LFD or HSB mice (P < 0.05), whereas there was no difference in expression levels between LFD and HSB mice.
31004166	9	2	theme	LFD	1183:1185	arg1	mice					1187:1190	LFD mice	1183:1190	LFD mice	1183:1190	RESULTS In HFD mice, body weight gain and hepatic triglyceride (TG), serum interleukin-6 (IL-6), and serum tumor necrosis factor (TNF)-α levels were 1-4 times higher than those in LFD mice (P < 0.05); they were 34-42% lower in HSB mice compared with HFD mice (P < 0.05).
31004166	14	3	theme	chronic	2105:2111	arg1	inflammation					2113:2124	low-grade chronic inflammation	2095:2124	low-grade chronic inflammation	2095:2124	These changes may correspond with improvements in obesity-related lipid accumulation and low-grade chronic inflammation.
31004166	12	4	theme	principal	1661:1669	arg1	analysis					1681:1688	principal component analysis	1661:1688	principal component analysis	1661:1688	Results from principal component analysis showed that HSB and LFD mice had a similar gut microbiota structure, which was significantly different from that in HFD mice (P < 0.05).
31004166	12	5	contain	had	1719:1721	arg1	mice					1714:1717	HSB and LFD mice	1702:1717	HSB and LFD mice	1702:1717	Results from principal component analysis showed that HSB and LFD mice had a similar gut microbiota structure, which was significantly different from that in HFD mice (P < 0.05).
31004166	12	5	contain	had	1719:1721	arg2	structure					1748:1756	a similar gut microbiota structure	1723:1756	a similar gut microbiota structure	1723:1756	Results from principal component analysis showed that HSB and LFD mice had a similar gut microbiota structure, which was significantly different from that in HFD mice (P < 0.05).
31004166	12	5	contain	had	1719:1721	arg2	different					1783:1791	different	1783:1791	different	1783:1791	Results from principal component analysis showed that HSB and LFD mice had a similar gut microbiota structure, which was significantly different from that in HFD mice (P < 0.05).
31004166	10	6	from	levels	1381:1386	arg1	P < 0.05					1408:1415	P < 0.05	1408:1415	P < 0.05	1408:1415	The HFD group had 28%-48% lower mRNA expression of both Tjp1 and Ocln in the ileum and colon compared with levels in LFD or HSB mice (P < 0.05), whereas there was no difference in expression levels between LFD and HSB mice.
31004166	10	6	from	levels	1381:1386	arg1	mice					1402:1405	LFD or HSB mice	1391:1405	mice	1402:1405	The HFD group had 28%-48% lower mRNA expression of both Tjp1 and Ocln in the ileum and colon compared with levels in LFD or HSB mice (P < 0.05), whereas there was no difference in expression levels between LFD and HSB mice.
31004166	10	7	theme	HFD	1278:1280	arg1	group					1282:1286	The HFD group	1274:1286	The HFD group	1274:1286	The HFD group had 28%-48% lower mRNA expression of both Tjp1 and Ocln in the ileum and colon compared with levels in LFD or HSB mice (P < 0.05), whereas there was no difference in expression levels between LFD and HSB mice.
31004166	9	8	theme	body	1024:1027	arg1	gain					1036:1039	body weight gain	1024:1039	body weight gain	1024:1039	RESULTS In HFD mice, body weight gain and hepatic triglyceride (TG), serum interleukin-6 (IL-6), and serum tumor necrosis factor (TNF)-α levels were 1-4 times higher than those in LFD mice (P < 0.05); they were 34-42% lower in HSB mice compared with HFD mice (P < 0.05).
31004166	9	8	theme	body	1024:1027	arg1	RESULTS					1003:1009	RESULTS	1003:1009	RESULTS In HFD mice	1003:1021	RESULTS In HFD mice, body weight gain and hepatic triglyceride (TG), serum interleukin-6 (IL-6), and serum tumor necrosis factor (TNF)-α levels were 1-4 times higher than those in LFD mice (P < 0.05); they were 34-42% lower in HSB mice compared with HFD mice (P < 0.05).
31004166	14	9	theme	lipid	2072:2076	arg1	accumulation					2078:2089	obesity-related lipid accumulation	2056:2089	obesity-related lipid accumulation	2056:2089	These changes may correspond with improvements in obesity-related lipid accumulation and low-grade chronic inflammation.
31004166	4	10	theme	%	595:595	arg1	energy					597:602	10% energy	593:602	LFD; 10% energy from fat	588:611	METHODS Male C57BL/6J mice, 4-5 wk of age, were divided into 3 groups (n = 8 mice/group): low-fat diet (LFD; 10% energy from fat), high-fat diet (HFD; 45% energy from fat), or high-fat diet plus sodium butyrate (HSB).
31004166	13	11	theme	LPS	1986:1988	arg1	concentrations					1990:2003	lower serum LPS concentrations	1974:2003	lower serum LPS concentrations	1974:2003	CONCLUSIONS Sodium butyrate administration beneficially changed HFD-induced gut microbiota composition and improved intestinal barrier, leading to lower serum LPS concentrations.
31004166	4	12	theme	low-fat	574:580	arg1	diet					582:585	low-fat diet	574:585	low-fat diet (LFD; 10% energy from fat)	574:612	METHODS Male C57BL/6J mice, 4-5 wk of age, were divided into 3 groups (n = 8 mice/group): low-fat diet (LFD; 10% energy from fat), high-fat diet (HFD; 45% energy from fat), or high-fat diet plus sodium butyrate (HSB).
31004166	4	12	theme	low-fat	574:580	arg1	groups					547:552	3 groups	545:552	3 groups (n = 8 mice/group): low-fat diet (LFD; 10% energy from fat), high-fat diet (HFD; 45% energy from fat), or high-fat diet plus sodium butyrate (HSB)	545:699	METHODS Male C57BL/6J mice, 4-5 wk of age, were divided into 3 groups (n = 8 mice/group): low-fat diet (LFD; 10% energy from fat), high-fat diet (HFD; 45% energy from fat), or high-fat diet plus sodium butyrate (HSB).
31004166	10	13	theme	%	1294:1294	arg1	%					1298:1298	28%-48%	1292:1298	28%-48%	1292:1298	The HFD group had 28%-48% lower mRNA expression of both Tjp1 and Ocln in the ileum and colon compared with levels in LFD or HSB mice (P < 0.05), whereas there was no difference in expression levels between LFD and HSB mice.
31004166	4	14	dep	groups	547:552	arg1	diet					582:585	low-fat diet	574:585	low-fat diet (LFD; 10% energy from fat)	574:612	METHODS Male C57BL/6J mice, 4-5 wk of age, were divided into 3 groups (n = 8 mice/group): low-fat diet (LFD; 10% energy from fat), high-fat diet (HFD; 45% energy from fat), or high-fat diet plus sodium butyrate (HSB).
31004166	4	14	dep	groups	547:552	arg1	groups					547:552	3 groups	545:552	3 groups (n = 8 mice/group): low-fat diet (LFD; 10% energy from fat), high-fat diet (HFD; 45% energy from fat), or high-fat diet plus sodium butyrate (HSB)	545:699	METHODS Male C57BL/6J mice, 4-5 wk of age, were divided into 3 groups (n = 8 mice/group): low-fat diet (LFD; 10% energy from fat), high-fat diet (HFD; 45% energy from fat), or high-fat diet plus sodium butyrate (HSB).
31004166	4	14	dep	groups	547:552	arg1	HSB					696:698	HSB	696:698	HSB	696:698	METHODS Male C57BL/6J mice, 4-5 wk of age, were divided into 3 groups (n = 8 mice/group): low-fat diet (LFD; 10% energy from fat), high-fat diet (HFD; 45% energy from fat), or high-fat diet plus sodium butyrate (HSB).
31004166	4	14	dep	groups	547:552	arg1	diet					669:672	high-fat diet	660:672	high-fat diet	660:672	METHODS Male C57BL/6J mice, 4-5 wk of age, were divided into 3 groups (n = 8 mice/group): low-fat diet (LFD; 10% energy from fat), high-fat diet (HFD; 45% energy from fat), or high-fat diet plus sodium butyrate (HSB).
31004166	4	14	dep	groups	547:552	arg1	diet					624:627	high-fat diet	615:627	high-fat diet (HFD; 45% energy from fat)	615:654	METHODS Male C57BL/6J mice, 4-5 wk of age, were divided into 3 groups (n = 8 mice/group): low-fat diet (LFD; 10% energy from fat), high-fat diet (HFD; 45% energy from fat), or high-fat diet plus sodium butyrate (HSB).
31004166	11	15	theme	LFD	1627:1629	arg1	P < 0.05					1637:1644	P < 0.05	1637:1644	P < 0.05	1637:1644	Furthermore, in HSB mice, serum LPS concentration was 53% lower compared with that in HFD mice but still 23% higher than that in LFD mice (P < 0.05).
31004166	11	15	theme	LFD	1627:1629	arg1	mice					1631:1634	LFD mice	1627:1634	LFD mice (P < 0.05)	1627:1645	Furthermore, in HSB mice, serum LPS concentration was 53% lower compared with that in HFD mice but still 23% higher than that in LFD mice (P < 0.05).
31004166	11	16	theme	LPS	1530:1532	arg1	concentration					1534:1546	serum LPS concentration	1524:1546	serum LPS concentration	1524:1546	Furthermore, in HSB mice, serum LPS concentration was 53% lower compared with that in HFD mice but still 23% higher than that in LFD mice (P < 0.05).
31004166	5	17	theme	M	762:762	arg1	concentration					741:753	a concentration	739:753	a concentration of 0.1 M in drinking water for 12 wk	739:790	HSB mice received sodium butyrate at a concentration of 0.1 M in drinking water for 12 wk.
31004166	14	18	from	improvements	2040:2051	arg1	inflammation					2113:2124	low-grade chronic inflammation	2095:2124	low-grade chronic inflammation	2095:2124	These changes may correspond with improvements in obesity-related lipid accumulation and low-grade chronic inflammation.
31004166	14	18	from	improvements	2040:2051	arg1	accumulation					2078:2089	obesity-related lipid accumulation	2056:2089	obesity-related lipid accumulation	2056:2089	These changes may correspond with improvements in obesity-related lipid accumulation and low-grade chronic inflammation.
31004166	3	19	theme	low-grade	401:409	arg1	inflammation					419:430	low-grade chronic inflammation	401:430	low-grade chronic inflammation	401:430	OBJECTIVE The aim of this study was to determine the role of butyrate, an SCFA, in the regulation of obesity, low-grade chronic inflammation, and alterations of microbiota composition in mice.
31004166	10	20	theme	expression	1454:1463	arg1	levels					1465:1470	expression levels	1454:1470	expression levels	1454:1470	The HFD group had 28%-48% lower mRNA expression of both Tjp1 and Ocln in the ileum and colon compared with levels in LFD or HSB mice (P < 0.05), whereas there was no difference in expression levels between LFD and HSB mice.
31004166	12	21	from	analysis	1681:1688	arg1	Results					1648:1654	Results	1648:1654	Results from principal component analysis	1648:1688	Results from principal component analysis showed that HSB and LFD mice had a similar gut microbiota structure, which was significantly different from that in HFD mice (P < 0.05).
31004166	5	22	theme	HSB	702:704	arg1	mice					706:709	HSB mice	702:709	HSB mice	702:709	HSB mice received sodium butyrate at a concentration of 0.1 M in drinking water for 12 wk.
31004166	4	23	theme	high-fat	615:622	arg1	groups					547:552	3 groups	545:552	3 groups (n = 8 mice/group): low-fat diet (LFD; 10% energy from fat), high-fat diet (HFD; 45% energy from fat), or high-fat diet plus sodium butyrate (HSB)	545:699	METHODS Male C57BL/6J mice, 4-5 wk of age, were divided into 3 groups (n = 8 mice/group): low-fat diet (LFD; 10% energy from fat), high-fat diet (HFD; 45% energy from fat), or high-fat diet plus sodium butyrate (HSB).
31004166	4	23	theme	high-fat	615:622	arg1	diet					624:627	high-fat diet	615:627	high-fat diet (HFD; 45% energy from fat)	615:654	METHODS Male C57BL/6J mice, 4-5 wk of age, were divided into 3 groups (n = 8 mice/group): low-fat diet (LFD; 10% energy from fat), high-fat diet (HFD; 45% energy from fat), or high-fat diet plus sodium butyrate (HSB).
31004166	10	24	theme	mRNA	1306:1309	arg1	expression					1311:1320	28%-48% lower mRNA expression	1292:1320	28%-48% lower mRNA expression of both Tjp1 and Ocln in the ileum and colon	1292:1365	The HFD group had 28%-48% lower mRNA expression of both Tjp1 and Ocln in the ileum and colon compared with levels in LFD or HSB mice (P < 0.05), whereas there was no difference in expression levels between LFD and HSB mice.
31004166	9	25	theme	HSB	1230:1232	arg1	mice					1234:1237	HSB mice	1230:1237	HSB mice	1230:1237	RESULTS In HFD mice, body weight gain and hepatic triglyceride (TG), serum interleukin-6 (IL-6), and serum tumor necrosis factor (TNF)-α levels were 1-4 times higher than those in LFD mice (P < 0.05); they were 34-42% lower in HSB mice compared with HFD mice (P < 0.05).
31004166	2	26	theme	further	268:274	arg1	investigation					276:288	further investigation	268:288	further investigation	268:288	However, the underlying mechanisms require further investigation.
31004166	7	27	theme	Serum	868:872	arg1	lipopolysaccharide					874:891	Serum lipopolysaccharide	868:891	Serum lipopolysaccharide (LPS) concentrations	868:912	Serum lipopolysaccharide (LPS) concentrations were measured in the 3 groups.
31004166	7	27	theme	Serum	868:872	arg1	LPS					894:896	LPS	894:896	LPS	894:896	Serum lipopolysaccharide (LPS) concentrations were measured in the 3 groups.
31004166	9	28	theme	serum	1072:1076	arg1	IL-6					1093:1096	IL-6	1093:1096	IL-6	1093:1096	RESULTS In HFD mice, body weight gain and hepatic triglyceride (TG), serum interleukin-6 (IL-6), and serum tumor necrosis factor (TNF)-α levels were 1-4 times higher than those in LFD mice (P < 0.05); they were 34-42% lower in HSB mice compared with HFD mice (P < 0.05).
31004166	9	28	theme	serum	1072:1076	arg1	interleukin-6					1078:1090	serum interleukin-6	1072:1090	serum interleukin-6 (IL-6)	1072:1097	RESULTS In HFD mice, body weight gain and hepatic triglyceride (TG), serum interleukin-6 (IL-6), and serum tumor necrosis factor (TNF)-α levels were 1-4 times higher than those in LFD mice (P < 0.05); they were 34-42% lower in HSB mice compared with HFD mice (P < 0.05).
31004166	4	29	dep	HFD	630:632	arg1	energy					639:644	45% energy	635:644	HFD; 45% energy from fat	630:653	METHODS Male C57BL/6J mice, 4-5 wk of age, were divided into 3 groups (n = 8 mice/group): low-fat diet (LFD; 10% energy from fat), high-fat diet (HFD; 45% energy from fat), or high-fat diet plus sodium butyrate (HSB).
31004166	2	30	theme	underlying	238:247	arg1	mechanisms					249:258	the underlying mechanisms	234:258	the underlying mechanisms	234:258	However, the underlying mechanisms require further investigation.
31004166	9	31	theme	HFD	1253:1255	arg1	P < 0.05					1263:1270	P < 0.05	1263:1270	P < 0.05	1263:1270	RESULTS In HFD mice, body weight gain and hepatic triglyceride (TG), serum interleukin-6 (IL-6), and serum tumor necrosis factor (TNF)-α levels were 1-4 times higher than those in LFD mice (P < 0.05); they were 34-42% lower in HSB mice compared with HFD mice (P < 0.05).
31004166	9	31	theme	HFD	1253:1255	arg1	mice					1257:1260	HFD mice	1253:1260	HFD mice (P < 0.05)	1253:1271	RESULTS In HFD mice, body weight gain and hepatic triglyceride (TG), serum interleukin-6 (IL-6), and serum tumor necrosis factor (TNF)-α levels were 1-4 times higher than those in LFD mice (P < 0.05); they were 34-42% lower in HSB mice compared with HFD mice (P < 0.05).
31004166	6	32	theme	intestinal	832:841	arg1	integrity					843:851	intestinal integrity	832:851	intestinal integrity	832:851	Measures of inflammation, obesity, and intestinal integrity were assessed.
31004166	14	33	theme	low-grade	2095:2103	arg1	inflammation					2113:2124	low-grade chronic inflammation	2095:2124	low-grade chronic inflammation	2095:2124	These changes may correspond with improvements in obesity-related lipid accumulation and low-grade chronic inflammation.
31004166	0	34	theme	Microbiota	74:83	arg1	Composition					51:61	the Composition	47:61	the Composition of the Gut Microbiota	47:83	Supplementation with Sodium Butyrate Modulates the Composition of the Gut Microbiota and Ameliorates High-Fat Diet-Induced Obesity in Mice.
31004166	13	35	theme	Sodium	1839:1844	arg1	butyrate					1846:1853	CONCLUSIONS Sodium butyrate	1827:1853	CONCLUSIONS Sodium butyrate administration	1827:1868	CONCLUSIONS Sodium butyrate administration beneficially changed HFD-induced gut microbiota composition and improved intestinal barrier, leading to lower serum LPS concentrations.
31004166	3	36	theme	butyrate	352:359	arg1	role					344:347	the role	340:347	the role of butyrate, an SCFA, in the regulation of obesity, low-grade chronic inflammation, and alterations of microbiota composition in mice	340:481	OBJECTIVE The aim of this study was to determine the role of butyrate, an SCFA, in the regulation of obesity, low-grade chronic inflammation, and alterations of microbiota composition in mice.
31004166	4	37	theme	Male	492:495	arg1	mice					506:509	Male C57BL/6J mice	492:509	METHODS Male C57BL/6J mice	484:509	METHODS Male C57BL/6J mice, 4-5 wk of age, were divided into 3 groups (n = 8 mice/group): low-fat diet (LFD; 10% energy from fat), high-fat diet (HFD; 45% energy from fat), or high-fat diet plus sodium butyrate (HSB).
31004166	1	38	theme	BACKGROUND	140:149	arg1	SCFAs					176:180	SCFAs	176:180	SCFAs	176:180	BACKGROUND Short-chain fatty acids (SCFAs) have been reported to ameliorate obesity.
31004166	1	38	theme	BACKGROUND	140:149	arg1	acids					169:173	BACKGROUND Short-chain fatty acids	140:173	BACKGROUND Short-chain fatty acids (SCFAs)	140:181	BACKGROUND Short-chain fatty acids (SCFAs) have been reported to ameliorate obesity.
31004166	3	39	theme	chronic	411:417	arg1	inflammation					419:430	low-grade chronic inflammation	401:430	low-grade chronic inflammation	401:430	OBJECTIVE The aim of this study was to determine the role of butyrate, an SCFA, in the regulation of obesity, low-grade chronic inflammation, and alterations of microbiota composition in mice.
31004166	0	40	theme	Diet-Induced	110:121	arg1	Obesity					123:129	High-Fat Diet-Induced Obesity	101:129	High-Fat Diet-Induced Obesity in Mice	101:137	Supplementation with Sodium Butyrate Modulates the Composition of the Gut Microbiota and Ameliorates High-Fat Diet-Induced Obesity in Mice.
31004166	9	41	theme	tumor	1110:1114	arg1	TNF					1133:1135	TNF	1133:1135	TNF	1133:1135	RESULTS In HFD mice, body weight gain and hepatic triglyceride (TG), serum interleukin-6 (IL-6), and serum tumor necrosis factor (TNF)-α levels were 1-4 times higher than those in LFD mice (P < 0.05); they were 34-42% lower in HSB mice compared with HFD mice (P < 0.05).
31004166	9	41	theme	tumor	1110:1114	arg1	factor					1125:1130	serum tumor necrosis factor	1104:1130	serum tumor necrosis factor (TNF)	1104:1136	RESULTS In HFD mice, body weight gain and hepatic triglyceride (TG), serum interleukin-6 (IL-6), and serum tumor necrosis factor (TNF)-α levels were 1-4 times higher than those in LFD mice (P < 0.05); they were 34-42% lower in HSB mice compared with HFD mice (P < 0.05).
31004166	11	42	theme	serum	1524:1528	arg1	concentration					1534:1546	serum LPS concentration	1524:1546	serum LPS concentration	1524:1546	Furthermore, in HSB mice, serum LPS concentration was 53% lower compared with that in HFD mice but still 23% higher than that in LFD mice (P < 0.05).
31004166	9	43	theme	factor	1125:1130	arg1	levels					1140:1145	hepatic triglyceride (TG), serum interleukin-6 (IL-6), and serum tumor necrosis factor (TNF)-α levels	1045:1145	hepatic triglyceride (TG), serum interleukin-6 (IL-6), and serum tumor necrosis factor (TNF)-α levels	1045:1145	RESULTS In HFD mice, body weight gain and hepatic triglyceride (TG), serum interleukin-6 (IL-6), and serum tumor necrosis factor (TNF)-α levels were 1-4 times higher than those in LFD mice (P < 0.05); they were 34-42% lower in HSB mice compared with HFD mice (P < 0.05).
31004166	9	43	theme	factor	1125:1130	arg1	RESULTS					1003:1009	RESULTS	1003:1009	RESULTS In HFD mice	1003:1021	RESULTS In HFD mice, body weight gain and hepatic triglyceride (TG), serum interleukin-6 (IL-6), and serum tumor necrosis factor (TNF)-α levels were 1-4 times higher than those in LFD mice (P < 0.05); they were 34-42% lower in HSB mice compared with HFD mice (P < 0.05).
31004166	4	44	dep	diet	624:627	arg1	HFD					630:632	HFD	630:632	HFD; 45% energy from fat	630:653	METHODS Male C57BL/6J mice, 4-5 wk of age, were divided into 3 groups (n = 8 mice/group): low-fat diet (LFD; 10% energy from fat), high-fat diet (HFD; 45% energy from fat), or high-fat diet plus sodium butyrate (HSB).
31004166	4	45	theme	%	637:637	arg1	energy					639:644	45% energy	635:644	HFD; 45% energy from fat	630:653	METHODS Male C57BL/6J mice, 4-5 wk of age, were divided into 3 groups (n = 8 mice/group): low-fat diet (LFD; 10% energy from fat), high-fat diet (HFD; 45% energy from fat), or high-fat diet plus sodium butyrate (HSB).
31004166	3	46	theme	alterations	437:447	arg1	regulation					378:387	the regulation	374:387	the regulation of obesity, low-grade chronic inflammation, and alterations of microbiota composition in mice	374:481	OBJECTIVE The aim of this study was to determine the role of butyrate, an SCFA, in the regulation of obesity, low-grade chronic inflammation, and alterations of microbiota composition in mice.
31004166	0	47	theme	Sodium	21:26	arg1	Butyrate					28:35	Sodium Butyrate	21:35	Sodium Butyrate	21:35	Supplementation with Sodium Butyrate Modulates the Composition of the Gut Microbiota and Ameliorates High-Fat Diet-Induced Obesity in Mice.
31004166	4	48	theme	sodium	679:684	arg1	groups					547:552	3 groups	545:552	3 groups (n = 8 mice/group): low-fat diet (LFD; 10% energy from fat), high-fat diet (HFD; 45% energy from fat), or high-fat diet plus sodium butyrate (HSB)	545:699	METHODS Male C57BL/6J mice, 4-5 wk of age, were divided into 3 groups (n = 8 mice/group): low-fat diet (LFD; 10% energy from fat), high-fat diet (HFD; 45% energy from fat), or high-fat diet plus sodium butyrate (HSB).
31004166	4	48	theme	sodium	679:684	arg1	butyrate					686:693	sodium butyrate	679:693	sodium butyrate	679:693	METHODS Male C57BL/6J mice, 4-5 wk of age, were divided into 3 groups (n = 8 mice/group): low-fat diet (LFD; 10% energy from fat), high-fat diet (HFD; 45% energy from fat), or high-fat diet plus sodium butyrate (HSB).
31004166	9	49	theme	-α	1137:1138	arg1	levels					1140:1145	hepatic triglyceride (TG), serum interleukin-6 (IL-6), and serum tumor necrosis factor (TNF)-α levels	1045:1145	hepatic triglyceride (TG), serum interleukin-6 (IL-6), and serum tumor necrosis factor (TNF)-α levels	1045:1145	RESULTS In HFD mice, body weight gain and hepatic triglyceride (TG), serum interleukin-6 (IL-6), and serum tumor necrosis factor (TNF)-α levels were 1-4 times higher than those in LFD mice (P < 0.05); they were 34-42% lower in HSB mice compared with HFD mice (P < 0.05).
31004166	9	49	theme	-α	1137:1138	arg1	RESULTS					1003:1009	RESULTS	1003:1009	RESULTS In HFD mice	1003:1021	RESULTS In HFD mice, body weight gain and hepatic triglyceride (TG), serum interleukin-6 (IL-6), and serum tumor necrosis factor (TNF)-α levels were 1-4 times higher than those in LFD mice (P < 0.05); they were 34-42% lower in HSB mice compared with HFD mice (P < 0.05).
31004166	4	50	dep	diet	582:585	arg1	LFD					588:590	LFD	588:590	LFD; 10% energy from fat	588:611	METHODS Male C57BL/6J mice, 4-5 wk of age, were divided into 3 groups (n = 8 mice/group): low-fat diet (LFD; 10% energy from fat), high-fat diet (HFD; 45% energy from fat), or high-fat diet plus sodium butyrate (HSB).
31004166	4	51	dep	METHODS	484:490	arg1	mice					506:509	Male C57BL/6J mice	492:509	METHODS Male C57BL/6J mice	484:509	METHODS Male C57BL/6J mice, 4-5 wk of age, were divided into 3 groups (n = 8 mice/group): low-fat diet (LFD; 10% energy from fat), high-fat diet (HFD; 45% energy from fat), or high-fat diet plus sodium butyrate (HSB).
31004166	4	52	theme	4-5	512:514	arg1	METHODS					484:490	METHODS Male C57BL/6J mice	484:509	METHODS Male C57BL/6J mice	484:509	METHODS Male C57BL/6J mice, 4-5 wk of age, were divided into 3 groups (n = 8 mice/group): low-fat diet (LFD; 10% energy from fat), high-fat diet (HFD; 45% energy from fat), or high-fat diet plus sodium butyrate (HSB).
31004166	4	52	theme	4-5	512:514	arg1	wk					516:517	4-5 wk	512:517	4-5 wk of age	512:524	METHODS Male C57BL/6J mice, 4-5 wk of age, were divided into 3 groups (n = 8 mice/group): low-fat diet (LFD; 10% energy from fat), high-fat diet (HFD; 45% energy from fat), or high-fat diet plus sodium butyrate (HSB).
31004166	12	53	theme	gut	1733:1735	arg1	different					1783:1791	different	1783:1791	different	1783:1791	Results from principal component analysis showed that HSB and LFD mice had a similar gut microbiota structure, which was significantly different from that in HFD mice (P < 0.05).
31004166	12	53	theme	gut	1733:1735	arg1	structure					1748:1756	a similar gut microbiota structure	1723:1756	a similar gut microbiota structure	1723:1756	Results from principal component analysis showed that HSB and LFD mice had a similar gut microbiota structure, which was significantly different from that in HFD mice (P < 0.05).
31004166	0	54	with	Supplementation	0:14	arg1	Butyrate					28:35	Sodium Butyrate	21:35	Sodium Butyrate	21:35	Supplementation with Sodium Butyrate Modulates the Composition of the Gut Microbiota and Ameliorates High-Fat Diet-Induced Obesity in Mice.
31004166	3	55	theme	microbiota	452:461	arg1	composition					463:473	microbiota composition	452:473	microbiota composition	452:473	OBJECTIVE The aim of this study was to determine the role of butyrate, an SCFA, in the regulation of obesity, low-grade chronic inflammation, and alterations of microbiota composition in mice.
31004166	8	56	theme	Fecal	945:949	arg1	samples					951:957	Fecal samples	945:957	Fecal samples	945:957	Fecal samples were collected for gut microbiota analysis.
31004166	1	57	theme	fatty	163:167	arg1	SCFAs					176:180	SCFAs	176:180	SCFAs	176:180	BACKGROUND Short-chain fatty acids (SCFAs) have been reported to ameliorate obesity.
31004166	1	57	theme	fatty	163:167	arg1	acids					169:173	BACKGROUND Short-chain fatty acids	140:173	BACKGROUND Short-chain fatty acids (SCFAs)	140:181	BACKGROUND Short-chain fatty acids (SCFAs) have been reported to ameliorate obesity.
31004166	9	58	theme	hepatic	1045:1051	arg1	TG					1067:1068	TG	1067:1068	TG	1067:1068	RESULTS In HFD mice, body weight gain and hepatic triglyceride (TG), serum interleukin-6 (IL-6), and serum tumor necrosis factor (TNF)-α levels were 1-4 times higher than those in LFD mice (P < 0.05); they were 34-42% lower in HSB mice compared with HFD mice (P < 0.05).
31004166	9	58	theme	hepatic	1045:1051	arg1	triglyceride					1053:1064	hepatic triglyceride	1045:1064	hepatic triglyceride (TG)	1045:1069	RESULTS In HFD mice, body weight gain and hepatic triglyceride (TG), serum interleukin-6 (IL-6), and serum tumor necrosis factor (TNF)-α levels were 1-4 times higher than those in LFD mice (P < 0.05); they were 34-42% lower in HSB mice compared with HFD mice (P < 0.05).
31004166	12	59	theme	LFD	1710:1712	arg1	mice					1714:1717	HSB and LFD mice	1702:1717	HSB and LFD mice	1702:1717	Results from principal component analysis showed that HSB and LFD mice had a similar gut microbiota structure, which was significantly different from that in HFD mice (P < 0.05).
31004166	4	60	dep	LFD	588:590	arg1	energy					597:602	10% energy	593:602	LFD; 10% energy from fat	588:611	METHODS Male C57BL/6J mice, 4-5 wk of age, were divided into 3 groups (n = 8 mice/group): low-fat diet (LFD; 10% energy from fat), high-fat diet (HFD; 45% energy from fat), or high-fat diet plus sodium butyrate (HSB).
31004166	3	61	dep	OBJECTIVE	291:299	arg1	was					323:325	was	323:325	was to determine the role of butyrate, an SCFA, in the regulation of obesity, low-grade chronic inflammation, and alterations of microbiota composition in mice	323:481	OBJECTIVE The aim of this study was to determine the role of butyrate, an SCFA, in the regulation of obesity, low-grade chronic inflammation, and alterations of microbiota composition in mice.
31004166	3	62	from	inflammation	419:430	arg1	mice					478:481	mice	478:481	mice	478:481	OBJECTIVE The aim of this study was to determine the role of butyrate, an SCFA, in the regulation of obesity, low-grade chronic inflammation, and alterations of microbiota composition in mice.
31004166	4	63	from	fat	609:611	arg1	energy					597:602	10% energy	593:602	LFD; 10% energy from fat	588:611	METHODS Male C57BL/6J mice, 4-5 wk of age, were divided into 3 groups (n = 8 mice/group): low-fat diet (LFD; 10% energy from fat), high-fat diet (HFD; 45% energy from fat), or high-fat diet plus sodium butyrate (HSB).
31004166	9	64	theme	HFD	1014:1016	arg1	mice					1018:1021	HFD mice	1014:1021	HFD mice	1014:1021	RESULTS In HFD mice, body weight gain and hepatic triglyceride (TG), serum interleukin-6 (IL-6), and serum tumor necrosis factor (TNF)-α levels were 1-4 times higher than those in LFD mice (P < 0.05); they were 34-42% lower in HSB mice compared with HFD mice (P < 0.05).
31004166	8	65	theme	microbiota	982:991	arg1	analysis					993:1000	gut microbiota analysis	978:1000	gut microbiota analysis	978:1000	Fecal samples were collected for gut microbiota analysis.
31004166	10	66	from	expression	1311:1320	arg1	ileum					1351:1355	ileum	1351:1355	ileum	1351:1355	The HFD group had 28%-48% lower mRNA expression of both Tjp1 and Ocln in the ileum and colon compared with levels in LFD or HSB mice (P < 0.05), whereas there was no difference in expression levels between LFD and HSB mice.
31004166	10	66	from	expression	1311:1320	arg1	colon					1361:1365	colon	1361:1365	colon	1361:1365	The HFD group had 28%-48% lower mRNA expression of both Tjp1 and Ocln in the ileum and colon compared with levels in LFD or HSB mice (P < 0.05), whereas there was no difference in expression levels between LFD and HSB mice.
31004166	13	67	theme	intestinal	1943:1952	arg1	barrier					1954:1960	intestinal barrier	1943:1960	intestinal barrier	1943:1960	CONCLUSIONS Sodium butyrate administration beneficially changed HFD-induced gut microbiota composition and improved intestinal barrier, leading to lower serum LPS concentrations.
31004166	9	68	from	RESULTS	1003:1009	arg1	mice					1018:1021	HFD mice	1014:1021	HFD mice	1014:1021	RESULTS In HFD mice, body weight gain and hepatic triglyceride (TG), serum interleukin-6 (IL-6), and serum tumor necrosis factor (TNF)-α levels were 1-4 times higher than those in LFD mice (P < 0.05); they were 34-42% lower in HSB mice compared with HFD mice (P < 0.05).
31004166	10	69	theme	lower	1300:1304	arg1	expression					1311:1320	28%-48% lower mRNA expression	1292:1320	28%-48% lower mRNA expression of both Tjp1 and Ocln in the ileum and colon	1292:1365	The HFD group had 28%-48% lower mRNA expression of both Tjp1 and Ocln in the ileum and colon compared with levels in LFD or HSB mice (P < 0.05), whereas there was no difference in expression levels between LFD and HSB mice.
31004166	9	70	theme	weight	1029:1034	arg1	gain					1036:1039	body weight gain	1024:1039	body weight gain	1024:1039	RESULTS In HFD mice, body weight gain and hepatic triglyceride (TG), serum interleukin-6 (IL-6), and serum tumor necrosis factor (TNF)-α levels were 1-4 times higher than those in LFD mice (P < 0.05); they were 34-42% lower in HSB mice compared with HFD mice (P < 0.05).
31004166	9	70	theme	weight	1029:1034	arg1	RESULTS					1003:1009	RESULTS	1003:1009	RESULTS In HFD mice	1003:1021	RESULTS In HFD mice, body weight gain and hepatic triglyceride (TG), serum interleukin-6 (IL-6), and serum tumor necrosis factor (TNF)-α levels were 1-4 times higher than those in LFD mice (P < 0.05); they were 34-42% lower in HSB mice compared with HFD mice (P < 0.05).
31004166	5	71	theme	sodium	720:725	arg1	butyrate					727:734	sodium butyrate	720:734	sodium butyrate	720:734	HSB mice received sodium butyrate at a concentration of 0.1 M in drinking water for 12 wk.
31004166	6	72	theme	inflammation	805:816	arg1	Measures					793:800	Measures	793:800	Measures of inflammation, obesity, and intestinal integrity	793:851	Measures of inflammation, obesity, and intestinal integrity were assessed.
31004166	4	73	theme	10	593:594	arg1	%					595:595	%	595:595	%	595:595	METHODS Male C57BL/6J mice, 4-5 wk of age, were divided into 3 groups (n = 8 mice/group): low-fat diet (LFD; 10% energy from fat), high-fat diet (HFD; 45% energy from fat), or high-fat diet plus sodium butyrate (HSB).
31004166	3	74	from	mice	478:481	arg1	regulation					378:387	the regulation	374:387	the regulation of obesity, low-grade chronic inflammation, and alterations of microbiota composition in mice	374:481	OBJECTIVE The aim of this study was to determine the role of butyrate, an SCFA, in the regulation of obesity, low-grade chronic inflammation, and alterations of microbiota composition in mice.
31004166	12	75	from	that	1798:1801	arg1	different					1783:1791	different	1783:1791	different	1783:1791	Results from principal component analysis showed that HSB and LFD mice had a similar gut microbiota structure, which was significantly different from that in HFD mice (P < 0.05).
31004166	12	75	from	that	1798:1801	arg1	structure					1748:1756	a similar gut microbiota structure	1723:1756	a similar gut microbiota structure	1723:1756	Results from principal component analysis showed that HSB and LFD mice had a similar gut microbiota structure, which was significantly different from that in HFD mice (P < 0.05).
31004166	13	76	theme	HFD-induced	1891:1901	arg1	composition					1918:1928	HFD-induced gut microbiota composition	1891:1928	HFD-induced gut microbiota composition	1891:1928	CONCLUSIONS Sodium butyrate administration beneficially changed HFD-induced gut microbiota composition and improved intestinal barrier, leading to lower serum LPS concentrations.
31004166	9	77	theme	triglyceride	1053:1064	arg1	levels					1140:1145	hepatic triglyceride (TG), serum interleukin-6 (IL-6), and serum tumor necrosis factor (TNF)-α levels	1045:1145	hepatic triglyceride (TG), serum interleukin-6 (IL-6), and serum tumor necrosis factor (TNF)-α levels	1045:1145	RESULTS In HFD mice, body weight gain and hepatic triglyceride (TG), serum interleukin-6 (IL-6), and serum tumor necrosis factor (TNF)-α levels were 1-4 times higher than those in LFD mice (P < 0.05); they were 34-42% lower in HSB mice compared with HFD mice (P < 0.05).
31004166	9	77	theme	triglyceride	1053:1064	arg1	RESULTS					1003:1009	RESULTS	1003:1009	RESULTS In HFD mice	1003:1021	RESULTS In HFD mice, body weight gain and hepatic triglyceride (TG), serum interleukin-6 (IL-6), and serum tumor necrosis factor (TNF)-α levels were 1-4 times higher than those in LFD mice (P < 0.05); they were 34-42% lower in HSB mice compared with HFD mice (P < 0.05).
31004166	13	78	theme	microbiota	1907:1916	arg1	composition					1918:1928	HFD-induced gut microbiota composition	1891:1928	HFD-induced gut microbiota composition	1891:1928	CONCLUSIONS Sodium butyrate administration beneficially changed HFD-induced gut microbiota composition and improved intestinal barrier, leading to lower serum LPS concentrations.
31004166	12	79	theme	HSB	1702:1704	arg1	mice					1714:1717	HSB and LFD mice	1702:1717	HSB and LFD mice	1702:1717	Results from principal component analysis showed that HSB and LFD mice had a similar gut microbiota structure, which was significantly different from that in HFD mice (P < 0.05).
31004166	7	80	theme	lipopolysaccharide	874:891	arg1	concentrations					899:912	Serum lipopolysaccharide (LPS) concentrations	868:912	Serum lipopolysaccharide (LPS) concentrations	868:912	Serum lipopolysaccharide (LPS) concentrations were measured in the 3 groups.
31004166	13	81	theme	butyrate	1846:1853	arg1	administration					1855:1868	CONCLUSIONS Sodium butyrate administration	1827:1868	CONCLUSIONS Sodium butyrate administration	1827:1868	CONCLUSIONS Sodium butyrate administration beneficially changed HFD-induced gut microbiota composition and improved intestinal barrier, leading to lower serum LPS concentrations.
31004166	3	82	theme	study	317:321	arg1	aim					305:307	The aim	301:307	The aim of this study	301:321	OBJECTIVE The aim of this study was to determine the role of butyrate, an SCFA, in the regulation of obesity, low-grade chronic inflammation, and alterations of microbiota composition in mice.
31004166	0	83	from	Obesity	123:129	arg1	Mice					134:137	Mice	134:137	Mice	134:137	Supplementation with Sodium Butyrate Modulates the Composition of the Gut Microbiota and Ameliorates High-Fat Diet-Induced Obesity in Mice.
31004166	10	84	theme	HSB	1488:1490	arg1	mice					1492:1495	LFD and HSB mice	1480:1495	LFD and HSB mice	1480:1495	The HFD group had 28%-48% lower mRNA expression of both Tjp1 and Ocln in the ileum and colon compared with levels in LFD or HSB mice (P < 0.05), whereas there was no difference in expression levels between LFD and HSB mice.
31004166	12	85	theme	component	1671:1679	arg1	analysis					1681:1688	principal component analysis	1661:1688	principal component analysis	1661:1688	Results from principal component analysis showed that HSB and LFD mice had a similar gut microbiota structure, which was significantly different from that in HFD mice (P < 0.05).
31004166	0	86	theme	Gut	70:72	arg1	Microbiota					74:83	the Gut Microbiota	66:83	the Gut Microbiota	66:83	Supplementation with Sodium Butyrate Modulates the Composition of the Gut Microbiota and Ameliorates High-Fat Diet-Induced Obesity in Mice.
31004166	9	87	theme	interleukin-6	1078:1090	arg1	levels					1140:1145	hepatic triglyceride (TG), serum interleukin-6 (IL-6), and serum tumor necrosis factor (TNF)-α levels	1045:1145	hepatic triglyceride (TG), serum interleukin-6 (IL-6), and serum tumor necrosis factor (TNF)-α levels	1045:1145	RESULTS In HFD mice, body weight gain and hepatic triglyceride (TG), serum interleukin-6 (IL-6), and serum tumor necrosis factor (TNF)-α levels were 1-4 times higher than those in LFD mice (P < 0.05); they were 34-42% lower in HSB mice compared with HFD mice (P < 0.05).
31004166	9	87	theme	interleukin-6	1078:1090	arg1	RESULTS					1003:1009	RESULTS	1003:1009	RESULTS In HFD mice	1003:1021	RESULTS In HFD mice, body weight gain and hepatic triglyceride (TG), serum interleukin-6 (IL-6), and serum tumor necrosis factor (TNF)-α levels were 1-4 times higher than those in LFD mice (P < 0.05); they were 34-42% lower in HSB mice compared with HFD mice (P < 0.05).
31004166	10	88	theme	LFD	1480:1482	arg1	mice					1492:1495	LFD and HSB mice	1480:1495	LFD and HSB mice	1480:1495	The HFD group had 28%-48% lower mRNA expression of both Tjp1 and Ocln in the ileum and colon compared with levels in LFD or HSB mice (P < 0.05), whereas there was no difference in expression levels between LFD and HSB mice.
31004166	1	89	theme	Short-chain	151:161	arg1	SCFAs					176:180	SCFAs	176:180	SCFAs	176:180	BACKGROUND Short-chain fatty acids (SCFAs) have been reported to ameliorate obesity.
31004166	1	89	theme	Short-chain	151:161	arg1	acids					169:173	BACKGROUND Short-chain fatty acids	140:173	BACKGROUND Short-chain fatty acids (SCFAs)	140:181	BACKGROUND Short-chain fatty acids (SCFAs) have been reported to ameliorate obesity.
31004166	6	90	theme	integrity	843:851	arg1	Measures					793:800	Measures	793:800	Measures of inflammation, obesity, and intestinal integrity	793:851	Measures of inflammation, obesity, and intestinal integrity were assessed.
31004166	11	91	theme	HSB	1514:1516	arg1	mice					1518:1521	HSB mice	1514:1521	HSB mice	1514:1521	Furthermore, in HSB mice, serum LPS concentration was 53% lower compared with that in HFD mice but still 23% higher than that in LFD mice (P < 0.05).
31004166	3	92	from	regulation	378:387	arg1	mice					478:481	mice	478:481	mice	478:481	OBJECTIVE The aim of this study was to determine the role of butyrate, an SCFA, in the regulation of obesity, low-grade chronic inflammation, and alterations of microbiota composition in mice.
31004166	13	93	theme	CONCLUSIONS	1827:1837	arg1	butyrate					1846:1853	CONCLUSIONS Sodium butyrate	1827:1853	CONCLUSIONS Sodium butyrate administration	1827:1868	CONCLUSIONS Sodium butyrate administration beneficially changed HFD-induced gut microbiota composition and improved intestinal barrier, leading to lower serum LPS concentrations.
31004166	4	94	theme	C57BL/6J	497:504	arg1	mice					506:509	Male C57BL/6J mice	492:509	METHODS Male C57BL/6J mice	484:509	METHODS Male C57BL/6J mice, 4-5 wk of age, were divided into 3 groups (n = 8 mice/group): low-fat diet (LFD; 10% energy from fat), high-fat diet (HFD; 45% energy from fat), or high-fat diet plus sodium butyrate (HSB).
31004166	3	95	from	role	344:347	arg1	regulation					378:387	the regulation	374:387	the regulation of obesity, low-grade chronic inflammation, and alterations of microbiota composition in mice	374:481	OBJECTIVE The aim of this study was to determine the role of butyrate, an SCFA, in the regulation of obesity, low-grade chronic inflammation, and alterations of microbiota composition in mice.
31004166	0	96	theme	High-Fat	101:108	arg1	Obesity					123:129	High-Fat Diet-Induced Obesity	101:129	High-Fat Diet-Induced Obesity in Mice	101:137	Supplementation with Sodium Butyrate Modulates the Composition of the Gut Microbiota and Ameliorates High-Fat Diet-Induced Obesity in Mice.
31004166	3	97	from	obesity	392:398	arg1	mice					478:481	mice	478:481	mice	478:481	OBJECTIVE The aim of this study was to determine the role of butyrate, an SCFA, in the regulation of obesity, low-grade chronic inflammation, and alterations of microbiota composition in mice.
31004166	4	98	from	fat	651:653	arg1	energy					639:644	45% energy	635:644	HFD; 45% energy from fat	630:653	METHODS Male C57BL/6J mice, 4-5 wk of age, were divided into 3 groups (n = 8 mice/group): low-fat diet (LFD; 10% energy from fat), high-fat diet (HFD; 45% energy from fat), or high-fat diet plus sodium butyrate (HSB).
31004166	6	99	theme	obesity	819:825	arg1	Measures					793:800	Measures	793:800	Measures of inflammation, obesity, and intestinal integrity	793:851	Measures of inflammation, obesity, and intestinal integrity were assessed.
31004166	12	100	theme	HFD	1806:1808	arg1	mice					1810:1813	HFD mice	1806:1813	HFD mice (P < 0.05)	1806:1824	Results from principal component analysis showed that HSB and LFD mice had a similar gut microbiota structure, which was significantly different from that in HFD mice (P < 0.05).
31004166	12	100	theme	HFD	1806:1808	arg1	P < 0.05					1816:1823	P < 0.05	1816:1823	P < 0.05	1816:1823	Results from principal component analysis showed that HSB and LFD mice had a similar gut microbiota structure, which was significantly different from that in HFD mice (P < 0.05).
31004166	9	101	theme	serum	1104:1108	arg1	TNF					1133:1135	TNF	1133:1135	TNF	1133:1135	RESULTS In HFD mice, body weight gain and hepatic triglyceride (TG), serum interleukin-6 (IL-6), and serum tumor necrosis factor (TNF)-α levels were 1-4 times higher than those in LFD mice (P < 0.05); they were 34-42% lower in HSB mice compared with HFD mice (P < 0.05).
31004166	9	101	theme	serum	1104:1108	arg1	factor					1125:1130	serum tumor necrosis factor	1104:1130	serum tumor necrosis factor (TNF)	1104:1136	RESULTS In HFD mice, body weight gain and hepatic triglyceride (TG), serum interleukin-6 (IL-6), and serum tumor necrosis factor (TNF)-α levels were 1-4 times higher than those in LFD mice (P < 0.05); they were 34-42% lower in HSB mice compared with HFD mice (P < 0.05).
31004166	13	102	theme	gut	1903:1905	arg1	composition					1918:1928	HFD-induced gut microbiota composition	1891:1928	HFD-induced gut microbiota composition	1891:1928	CONCLUSIONS Sodium butyrate administration beneficially changed HFD-induced gut microbiota composition and improved intestinal barrier, leading to lower serum LPS concentrations.
31004166	10	103	from	difference	1440:1449	arg1	levels					1465:1470	expression levels	1454:1470	expression levels	1454:1470	The HFD group had 28%-48% lower mRNA expression of both Tjp1 and Ocln in the ileum and colon compared with levels in LFD or HSB mice (P < 0.05), whereas there was no difference in expression levels between LFD and HSB mice.
31004166	3	104	theme	inflammation	419:430	arg1	regulation					378:387	the regulation	374:387	the regulation of obesity, low-grade chronic inflammation, and alterations of microbiota composition in mice	374:481	OBJECTIVE The aim of this study was to determine the role of butyrate, an SCFA, in the regulation of obesity, low-grade chronic inflammation, and alterations of microbiota composition in mice.
31004166	9	105	theme	necrosis	1116:1123	arg1	TNF					1133:1135	TNF	1133:1135	TNF	1133:1135	RESULTS In HFD mice, body weight gain and hepatic triglyceride (TG), serum interleukin-6 (IL-6), and serum tumor necrosis factor (TNF)-α levels were 1-4 times higher than those in LFD mice (P < 0.05); they were 34-42% lower in HSB mice compared with HFD mice (P < 0.05).
31004166	9	105	theme	necrosis	1116:1123	arg1	factor					1125:1130	serum tumor necrosis factor	1104:1130	serum tumor necrosis factor (TNF)	1104:1136	RESULTS In HFD mice, body weight gain and hepatic triglyceride (TG), serum interleukin-6 (IL-6), and serum tumor necrosis factor (TNF)-α levels were 1-4 times higher than those in LFD mice (P < 0.05); they were 34-42% lower in HSB mice compared with HFD mice (P < 0.05).
31004166	4	106	theme	age	522:524	arg1	METHODS					484:490	METHODS Male C57BL/6J mice	484:509	METHODS Male C57BL/6J mice	484:509	METHODS Male C57BL/6J mice, 4-5 wk of age, were divided into 3 groups (n = 8 mice/group): low-fat diet (LFD; 10% energy from fat), high-fat diet (HFD; 45% energy from fat), or high-fat diet plus sodium butyrate (HSB).
31004166	4	106	theme	age	522:524	arg1	wk					516:517	4-5 wk	512:517	4-5 wk of age	512:524	METHODS Male C57BL/6J mice, 4-5 wk of age, were divided into 3 groups (n = 8 mice/group): low-fat diet (LFD; 10% energy from fat), high-fat diet (HFD; 45% energy from fat), or high-fat diet plus sodium butyrate (HSB).
31004166	10	107	theme	Tjp1	1330:1333	arg1	expression					1311:1320	28%-48% lower mRNA expression	1292:1320	28%-48% lower mRNA expression of both Tjp1 and Ocln in the ileum and colon	1292:1365	The HFD group had 28%-48% lower mRNA expression of both Tjp1 and Ocln in the ileum and colon compared with levels in LFD or HSB mice (P < 0.05), whereas there was no difference in expression levels between LFD and HSB mice.
31004166	10	108	contain	had	1288:1290	arg1	group					1282:1286	The HFD group	1274:1286	The HFD group	1274:1286	The HFD group had 28%-48% lower mRNA expression of both Tjp1 and Ocln in the ileum and colon compared with levels in LFD or HSB mice (P < 0.05), whereas there was no difference in expression levels between LFD and HSB mice.
31004166	10	108	contain	had	1288:1290	arg2	expression					1311:1320	28%-48% lower mRNA expression	1292:1320	28%-48% lower mRNA expression of both Tjp1 and Ocln in the ileum and colon	1292:1365	The HFD group had 28%-48% lower mRNA expression of both Tjp1 and Ocln in the ileum and colon compared with levels in LFD or HSB mice (P < 0.05), whereas there was no difference in expression levels between LFD and HSB mice.
31004166	4	109	theme	45	635:636	arg1	%					637:637	%	637:637	%	637:637	METHODS Male C57BL/6J mice, 4-5 wk of age, were divided into 3 groups (n = 8 mice/group): low-fat diet (LFD; 10% energy from fat), high-fat diet (HFD; 45% energy from fat), or high-fat diet plus sodium butyrate (HSB).
31004166	13	110	theme	serum	1980:1984	arg1	concentrations					1990:2003	lower serum LPS concentrations	1974:2003	lower serum LPS concentrations	1974:2003	CONCLUSIONS Sodium butyrate administration beneficially changed HFD-induced gut microbiota composition and improved intestinal barrier, leading to lower serum LPS concentrations.
31004166	3	111	theme	obesity	392:398	arg1	regulation					378:387	the regulation	374:387	the regulation of obesity, low-grade chronic inflammation, and alterations of microbiota composition in mice	374:481	OBJECTIVE The aim of this study was to determine the role of butyrate, an SCFA, in the regulation of obesity, low-grade chronic inflammation, and alterations of microbiota composition in mice.
31004166	14	112	theme	obesity-related	2056:2070	arg1	accumulation					2078:2089	obesity-related lipid accumulation	2056:2089	obesity-related lipid accumulation	2056:2089	These changes may correspond with improvements in obesity-related lipid accumulation and low-grade chronic inflammation.
31004166	13	113	theme	lower	1974:1978	arg1	concentrations					1990:2003	lower serum LPS concentrations	1974:2003	lower serum LPS concentrations	1974:2003	CONCLUSIONS Sodium butyrate administration beneficially changed HFD-induced gut microbiota composition and improved intestinal barrier, leading to lower serum LPS concentrations.
31004166	3	114	from	alterations	437:447	arg1	mice					478:481	mice	478:481	mice	478:481	OBJECTIVE The aim of this study was to determine the role of butyrate, an SCFA, in the regulation of obesity, low-grade chronic inflammation, and alterations of microbiota composition in mice.
31004166	12	115	theme	microbiota	1737:1746	arg1	different					1783:1791	different	1783:1791	different	1783:1791	Results from principal component analysis showed that HSB and LFD mice had a similar gut microbiota structure, which was significantly different from that in HFD mice (P < 0.05).
31004166	12	115	theme	microbiota	1737:1746	arg1	structure					1748:1756	a similar gut microbiota structure	1723:1756	a similar gut microbiota structure	1723:1756	Results from principal component analysis showed that HSB and LFD mice had a similar gut microbiota structure, which was significantly different from that in HFD mice (P < 0.05).
31004166	11	116	theme	HFD	1584:1586	arg1	mice					1588:1591	HFD mice	1584:1591	HFD mice	1584:1591	Furthermore, in HSB mice, serum LPS concentration was 53% lower compared with that in HFD mice but still 23% higher than that in LFD mice (P < 0.05).
31004166	12	117	theme	similar	1725:1731	arg1	different					1783:1791	different	1783:1791	different	1783:1791	Results from principal component analysis showed that HSB and LFD mice had a similar gut microbiota structure, which was significantly different from that in HFD mice (P < 0.05).
31004166	12	117	theme	similar	1725:1731	arg1	structure					1748:1756	a similar gut microbiota structure	1723:1756	a similar gut microbiota structure	1723:1756	Results from principal component analysis showed that HSB and LFD mice had a similar gut microbiota structure, which was significantly different from that in HFD mice (P < 0.05).
31004166	3	118	theme	composition	463:473	arg1	inflammation					419:430	low-grade chronic inflammation	401:430	low-grade chronic inflammation	401:430	OBJECTIVE The aim of this study was to determine the role of butyrate, an SCFA, in the regulation of obesity, low-grade chronic inflammation, and alterations of microbiota composition in mice.
31004166	3	118	theme	composition	463:473	arg1	alterations					437:447	alterations	437:447	alterations of microbiota composition in mice	437:481	OBJECTIVE The aim of this study was to determine the role of butyrate, an SCFA, in the regulation of obesity, low-grade chronic inflammation, and alterations of microbiota composition in mice.
31004166	3	118	theme	composition	463:473	arg1	obesity					392:398	obesity	392:398	obesity	392:398	OBJECTIVE The aim of this study was to determine the role of butyrate, an SCFA, in the regulation of obesity, low-grade chronic inflammation, and alterations of microbiota composition in mice.
31004166	10	119	theme	Ocln	1339:1342	arg1	expression					1311:1320	28%-48% lower mRNA expression	1292:1320	28%-48% lower mRNA expression of both Tjp1 and Ocln in the ileum and colon	1292:1365	The HFD group had 28%-48% lower mRNA expression of both Tjp1 and Ocln in the ileum and colon compared with levels in LFD or HSB mice (P < 0.05), whereas there was no difference in expression levels between LFD and HSB mice.
31004166	4	120	theme	high-fat	660:667	arg1	groups					547:552	3 groups	545:552	3 groups (n = 8 mice/group): low-fat diet (LFD; 10% energy from fat), high-fat diet (HFD; 45% energy from fat), or high-fat diet plus sodium butyrate (HSB)	545:699	METHODS Male C57BL/6J mice, 4-5 wk of age, were divided into 3 groups (n = 8 mice/group): low-fat diet (LFD; 10% energy from fat), high-fat diet (HFD; 45% energy from fat), or high-fat diet plus sodium butyrate (HSB).
31004166	4	120	theme	high-fat	660:667	arg1	HSB					696:698	HSB	696:698	HSB	696:698	METHODS Male C57BL/6J mice, 4-5 wk of age, were divided into 3 groups (n = 8 mice/group): low-fat diet (LFD; 10% energy from fat), high-fat diet (HFD; 45% energy from fat), or high-fat diet plus sodium butyrate (HSB).
31004166	4	120	theme	high-fat	660:667	arg1	diet					669:672	high-fat diet	660:672	high-fat diet	660:672	METHODS Male C57BL/6J mice, 4-5 wk of age, were divided into 3 groups (n = 8 mice/group): low-fat diet (LFD; 10% energy from fat), high-fat diet (HFD; 45% energy from fat), or high-fat diet plus sodium butyrate (HSB).
31004166	10	121	theme	HSB	1398:1400	arg1	P < 0.05					1408:1415	P < 0.05	1408:1415	P < 0.05	1408:1415	The HFD group had 28%-48% lower mRNA expression of both Tjp1 and Ocln in the ileum and colon compared with levels in LFD or HSB mice (P < 0.05), whereas there was no difference in expression levels between LFD and HSB mice.
31004166	10	121	theme	HSB	1398:1400	arg1	mice					1402:1405	LFD or HSB mice	1391:1405	mice	1402:1405	The HFD group had 28%-48% lower mRNA expression of both Tjp1 and Ocln in the ileum and colon compared with levels in LFD or HSB mice (P < 0.05), whereas there was no difference in expression levels between LFD and HSB mice.
30703427	0	0	theme	second-generation	96:112	arg1	process					133:139	the second-generation ethanol production process	92:139	the second-generation ethanol production process	92:139	Isolation and physicochemical characterization of different lignin streams generated during the second-generation ethanol production process.
30703427	8	1	attach	isolated	1368:1375	arg1	processing					1393:1402	rice straw processing	1382:1402	rice straw processing	1382:1402	Interesting properties and characteristics were observed for all the lignin samples isolated from rice straw processing, which would allow their application in different industrial areas.
30703427	8	1	attach	isolated	1368:1375	arg2	samples					1360:1366	all the lignin samples	1345:1366	all the lignin samples isolated from rice straw processing, which would allow their application in different industrial areas	1345:1469	Interesting properties and characteristics were observed for all the lignin samples isolated from rice straw processing, which would allow their application in different industrial areas.
30703427	6	2	theme	fermentation	998:1009	arg1	residues					1011:1018	the fermentation residues	994:1018	the fermentation residues	994:1018	In general, the fermentation residues contained non-hydrolyzed polysaccharides in their composition and lower lignin content when compared to the lignin solubilized in the alkaline liquor.
30703427	2	3	theme	components	504:513	arg1	terms					490:494	terms	490:494	terms of main components (lignin, carbohydrates, among others) and elemental composition	490:577	Then, a complete chemical characterization in terms of main components (lignin, carbohydrates, among others) and elemental composition was performed for all the samples.
30703427	2	4	from	characterization	470:485	arg1	terms					490:494	terms	490:494	terms of main components (lignin, carbohydrates, among others) and elemental composition	490:577	Then, a complete chemical characterization in terms of main components (lignin, carbohydrates, among others) and elemental composition was performed for all the samples.
30703427	0	5	theme	production	122:131	arg1	process					133:139	the second-generation ethanol production process	92:139	the second-generation ethanol production process	92:139	Isolation and physicochemical characterization of different lignin streams generated during the second-generation ethanol production process.
30703427	6	6	from	polysaccharides	1045:1059	arg1	composition					1070:1080	their composition	1064:1080	their composition	1064:1080	In general, the fermentation residues contained non-hydrolyzed polysaccharides in their composition and lower lignin content when compared to the lignin solubilized in the alkaline liquor.
30703427	6	6	from	polysaccharides	1045:1059	arg1	content					1099:1105	lower lignin content	1086:1105	lower lignin content	1086:1105	In general, the fermentation residues contained non-hydrolyzed polysaccharides in their composition and lower lignin content when compared to the lignin solubilized in the alkaline liquor.
30703427	0	7	theme	ethanol	114:120	arg1	process					133:139	the second-generation ethanol production process	92:139	the second-generation ethanol production process	92:139	Isolation and physicochemical characterization of different lignin streams generated during the second-generation ethanol production process.
30703427	6	8	contain	contained	1020:1028	arg2	polysaccharides					1045:1059	non-hydrolyzed polysaccharides	1030:1059	non-hydrolyzed polysaccharides in their composition and lower lignin content	1030:1105	In general, the fermentation residues contained non-hydrolyzed polysaccharides in their composition and lower lignin content when compared to the lignin solubilized in the alkaline liquor.
30703427	6	8	contain	contained	1020:1028	arg1	general					985:991	general	985:991	general	985:991	In general, the fermentation residues contained non-hydrolyzed polysaccharides in their composition and lower lignin content when compared to the lignin solubilized in the alkaline liquor.
30703427	6	8	contain	contained	1020:1028	arg2	residues					1011:1018	the fermentation residues	994:1018	the fermentation residues	994:1018	In general, the fermentation residues contained non-hydrolyzed polysaccharides in their composition and lower lignin content when compared to the lignin solubilized in the alkaline liquor.
30703427	6	8	contain	contained	1020:1028	arg1	residues					1011:1018	the fermentation residues	994:1018	the fermentation residues	994:1018	In general, the fermentation residues contained non-hydrolyzed polysaccharides in their composition and lower lignin content when compared to the lignin solubilized in the alkaline liquor.
30703427	5	9	theme	sample	974:979	arg1	properties					956:965	the final properties	946:965	the final properties of the sample	946:979	The results revealed that the process used for lignin isolation has an important influence in the final properties of the sample.
30703427	1	10	theme	fermentation	384:395	arg1	fermentation					370:381	fermentation	370:381	fermentation (fermentation residues)	370:405	Four different lignin samples were isolated during the processing of rice straw for ethanol production: one after mild alkaline pretreatment (deacetylation) of rice straw, and other three after simultaneous saccharification and fermentation (fermentation residues), under different process conditions.
30703427	1	10	theme	fermentation	384:395	arg1	residues					397:404	fermentation residues	384:404	fermentation residues	384:404	Four different lignin samples were isolated during the processing of rice straw for ethanol production: one after mild alkaline pretreatment (deacetylation) of rice straw, and other three after simultaneous saccharification and fermentation (fermentation residues), under different process conditions.
30703427	4	11	theme	standard	803:810	arg1	lignin					812:817	a standard lignin	801:817	a standard lignin (Kraft lignin)	801:832	For comparison, a standard lignin (Kraft lignin) was also assayed.
30703427	4	11	theme	standard	803:810	arg1	lignin					826:831	Kraft lignin	820:831	Kraft lignin	820:831	For comparison, a standard lignin (Kraft lignin) was also assayed.
30703427	6	12	theme	lignin	1092:1097	arg1	content					1099:1105	lower lignin content	1086:1105	lower lignin content	1086:1105	In general, the fermentation residues contained non-hydrolyzed polysaccharides in their composition and lower lignin content when compared to the lignin solubilized in the alkaline liquor.
30703427	7	13	theme	acetone	1216:1222	arg1	efficient					1247:1255	efficient	1247:1255	efficient	1247:1255	Among several organic solvents and mixtures, acetone 80% (v/v) was the most efficient for lignin solubilisation.
30703427	7	13	theme	acetone	1216:1222	arg1	v/v					1229:1231	v/v	1229:1231	v/v	1229:1231	Among several organic solvents and mixtures, acetone 80% (v/v) was the most efficient for lignin solubilisation.
30703427	7	13	theme	acetone	1216:1222	arg1	%					1226:1226	acetone 80%	1216:1226	acetone 80% (v/v)	1216:1232	Among several organic solvents and mixtures, acetone 80% (v/v) was the most efficient for lignin solubilisation.
30703427	8	14	theme	rice	1382:1385	arg1	processing					1393:1402	rice straw processing	1382:1402	rice straw processing	1382:1402	Interesting properties and characteristics were observed for all the lignin samples isolated from rice straw processing, which would allow their application in different industrial areas.
30703427	2	15	theme	main	499:502	arg1	components					504:513	main components	499:513	main components (lignin, carbohydrates, among others)	499:551	Then, a complete chemical characterization in terms of main components (lignin, carbohydrates, among others) and elemental composition was performed for all the samples.
30703427	3	16	theme	antioxidant	741:751	arg1	potential					753:761	antioxidant potential	741:761	antioxidant potential	741:761	Additionally, the structural and morphological characteristics, calorific value, thermal stability, solubility in solvents and antioxidant potential were also determined.
30703427	8	17	theme	straw	1387:1391	arg1	processing					1393:1402	rice straw processing	1382:1402	rice straw processing	1382:1402	Interesting properties and characteristics were observed for all the lignin samples isolated from rice straw processing, which would allow their application in different industrial areas.
30703427	1	18	theme	rice	211:214	arg1	straw					216:220	rice straw	211:220	rice straw	211:220	Four different lignin samples were isolated during the processing of rice straw for ethanol production: one after mild alkaline pretreatment (deacetylation) of rice straw, and other three after simultaneous saccharification and fermentation (fermentation residues), under different process conditions.
30703427	2	19	theme	chemical	461:468	arg1	characterization					470:485	a complete chemical characterization	450:485	a complete chemical characterization in terms of main components (lignin, carbohydrates, among others) and elemental composition	450:577	Then, a complete chemical characterization in terms of main components (lignin, carbohydrates, among others) and elemental composition was performed for all the samples.
30703427	5	20	theme	final	950:954	arg1	properties					956:965	the final properties	946:965	the final properties of the sample	946:979	The results revealed that the process used for lignin isolation has an important influence in the final properties of the sample.
30703427	1	21	theme	straw	216:220	arg1	processing					197:206	the processing	193:206	the processing of rice straw for ethanol production	193:243	Four different lignin samples were isolated during the processing of rice straw for ethanol production: one after mild alkaline pretreatment (deacetylation) of rice straw, and other three after simultaneous saccharification and fermentation (fermentation residues), under different process conditions.
30703427	2	22	theme	complete	452:459	arg1	characterization					470:485	a complete chemical characterization	450:485	a complete chemical characterization in terms of main components (lignin, carbohydrates, among others) and elemental composition	450:577	Then, a complete chemical characterization in terms of main components (lignin, carbohydrates, among others) and elemental composition was performed for all the samples.
30703427	1	23	theme	rice	302:305	arg1	straw					307:311	rice straw	302:311	rice straw	302:311	Four different lignin samples were isolated during the processing of rice straw for ethanol production: one after mild alkaline pretreatment (deacetylation) of rice straw, and other three after simultaneous saccharification and fermentation (fermentation residues), under different process conditions.
30703427	2	24	theme	composition	567:577	arg1	terms					490:494	terms	490:494	terms of main components (lignin, carbohydrates, among others) and elemental composition	490:577	Then, a complete chemical characterization in terms of main components (lignin, carbohydrates, among others) and elemental composition was performed for all the samples.
30703427	0	25	theme	physicochemical	14:28	arg1	characterization					30:45	physicochemical characterization	14:45	physicochemical characterization	14:45	Isolation and physicochemical characterization of different lignin streams generated during the second-generation ethanol production process.
30703427	1	26	theme	straw	307:311	arg1	deacetylation					284:296	deacetylation	284:296	deacetylation	284:296	Four different lignin samples were isolated during the processing of rice straw for ethanol production: one after mild alkaline pretreatment (deacetylation) of rice straw, and other three after simultaneous saccharification and fermentation (fermentation residues), under different process conditions.
30703427	1	26	theme	straw	307:311	arg1	pretreatment					270:281	mild alkaline pretreatment	256:281	mild alkaline pretreatment (deacetylation) of rice straw	256:311	Four different lignin samples were isolated during the processing of rice straw for ethanol production: one after mild alkaline pretreatment (deacetylation) of rice straw, and other three after simultaneous saccharification and fermentation (fermentation residues), under different process conditions.
30703427	2	27	theme	elemental	557:565	arg1	composition					567:577	elemental composition	557:577	elemental composition	557:577	Then, a complete chemical characterization in terms of main components (lignin, carbohydrates, among others) and elemental composition was performed for all the samples.
30703427	8	28	from	application	1429:1439	arg1	areas					1465:1469	different industrial areas	1444:1469	different industrial areas	1444:1469	Interesting properties and characteristics were observed for all the lignin samples isolated from rice straw processing, which would allow their application in different industrial areas.
30703427	3	29	theme	morphological	647:659	arg1	characteristics					661:675	the structural and morphological characteristics	628:675	the structural and morphological characteristics	628:675	Additionally, the structural and morphological characteristics, calorific value, thermal stability, solubility in solvents and antioxidant potential were also determined.
30703427	1	30	theme	ethanol	226:232	arg1	production					234:243	ethanol production	226:243	ethanol production	226:243	Four different lignin samples were isolated during the processing of rice straw for ethanol production: one after mild alkaline pretreatment (deacetylation) of rice straw, and other three after simultaneous saccharification and fermentation (fermentation residues), under different process conditions.
30703427	0	31	theme	different	50:58	arg1	streams					67:73	different lignin streams	50:73	different lignin streams	50:73	Isolation and physicochemical characterization of different lignin streams generated during the second-generation ethanol production process.
30703427	1	32	theme	different	147:155	arg1	samples					164:170	Four different lignin samples	142:170	Four different lignin samples	142:170	Four different lignin samples were isolated during the processing of rice straw for ethanol production: one after mild alkaline pretreatment (deacetylation) of rice straw, and other three after simultaneous saccharification and fermentation (fermentation residues), under different process conditions.
30703427	3	33	from	stability	703:711	arg1	solvents					728:735	solvents	728:735	solvents	728:735	Additionally, the structural and morphological characteristics, calorific value, thermal stability, solubility in solvents and antioxidant potential were also determined.
30703427	3	34	theme	calorific	678:686	arg1	value					688:692	calorific value	678:692	calorific value	678:692	Additionally, the structural and morphological characteristics, calorific value, thermal stability, solubility in solvents and antioxidant potential were also determined.
30703427	6	35	theme	lower	1086:1090	arg1	content					1099:1105	lower lignin content	1086:1105	lower lignin content	1086:1105	In general, the fermentation residues contained non-hydrolyzed polysaccharides in their composition and lower lignin content when compared to the lignin solubilized in the alkaline liquor.
30703427	8	36	theme	Interesting	1284:1294	arg1	properties					1296:1305	Interesting properties	1284:1305	Interesting properties	1284:1305	Interesting properties and characteristics were observed for all the lignin samples isolated from rice straw processing, which would allow their application in different industrial areas.
30703427	3	37	from	solubility	714:723	arg1	solvents					728:735	solvents	728:735	solvents	728:735	Additionally, the structural and morphological characteristics, calorific value, thermal stability, solubility in solvents and antioxidant potential were also determined.
30703427	7	38	theme	several	1177:1183	arg1	solvents					1193:1200	several organic solvents	1177:1200	several organic solvents	1177:1200	Among several organic solvents and mixtures, acetone 80% (v/v) was the most efficient for lignin solubilisation.
30703427	1	39	dep	one	246:248	arg1	conditions					432:441	different process conditions	414:441	different process conditions	414:441	Four different lignin samples were isolated during the processing of rice straw for ethanol production: one after mild alkaline pretreatment (deacetylation) of rice straw, and other three after simultaneous saccharification and fermentation (fermentation residues), under different process conditions.
30703427	3	40	from	potential	753:761	arg1	solvents					728:735	solvents	728:735	solvents	728:735	Additionally, the structural and morphological characteristics, calorific value, thermal stability, solubility in solvents and antioxidant potential were also determined.
30703427	9	41	theme	potential	1497:1505	arg1	applications					1507:1518	potential applications	1497:1518	potential applications	1497:1518	Based on the properties, potential applications were suggested, which could be of value for integration in biorefineries.
30703427	0	42	theme	streams	67:73	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation and physicochemical characterization of different lignin streams generated during the second-generation ethanol production process.
30703427	0	42	theme	streams	67:73	arg1	characterization					30:45	physicochemical characterization	14:45	physicochemical characterization	14:45	Isolation and physicochemical characterization of different lignin streams generated during the second-generation ethanol production process.
30703427	1	43	theme	process	424:430	arg1	conditions					432:441	different process conditions	414:441	different process conditions	414:441	Four different lignin samples were isolated during the processing of rice straw for ethanol production: one after mild alkaline pretreatment (deacetylation) of rice straw, and other three after simultaneous saccharification and fermentation (fermentation residues), under different process conditions.
30703427	7	44	theme	lignin	1261:1266	arg1	solubilisation					1268:1281	lignin solubilisation	1261:1281	lignin solubilisation	1261:1281	Among several organic solvents and mixtures, acetone 80% (v/v) was the most efficient for lignin solubilisation.
30703427	6	45	theme	alkaline	1154:1161	arg1	liquor					1163:1168	the alkaline liquor	1150:1168	the alkaline liquor	1150:1168	In general, the fermentation residues contained non-hydrolyzed polysaccharides in their composition and lower lignin content when compared to the lignin solubilized in the alkaline liquor.
30703427	0	46	theme	lignin	60:65	arg1	streams					67:73	different lignin streams	50:73	different lignin streams	50:73	Isolation and physicochemical characterization of different lignin streams generated during the second-generation ethanol production process.
30703427	2	47	dep	components	504:513	arg1	lignin					516:521	lignin	516:521	lignin	516:521	Then, a complete chemical characterization in terms of main components (lignin, carbohydrates, among others) and elemental composition was performed for all the samples.
30703427	2	47	dep	components	504:513	arg1	carbohydrates					524:536	carbohydrates	524:536	carbohydrates	524:536	Then, a complete chemical characterization in terms of main components (lignin, carbohydrates, among others) and elemental composition was performed for all the samples.
30703427	3	48	theme	thermal	695:701	arg1	stability					703:711	thermal stability	695:711	thermal stability	695:711	Additionally, the structural and morphological characteristics, calorific value, thermal stability, solubility in solvents and antioxidant potential were also determined.
30703427	8	49	theme	different	1444:1452	arg1	areas					1465:1469	different industrial areas	1444:1469	different industrial areas	1444:1469	Interesting properties and characteristics were observed for all the lignin samples isolated from rice straw processing, which would allow their application in different industrial areas.
30703427	1	50	theme	different	414:422	arg1	conditions					432:441	different process conditions	414:441	different process conditions	414:441	Four different lignin samples were isolated during the processing of rice straw for ethanol production: one after mild alkaline pretreatment (deacetylation) of rice straw, and other three after simultaneous saccharification and fermentation (fermentation residues), under different process conditions.
30703427	7	51	theme	organic	1185:1191	arg1	solvents					1193:1200	several organic solvents	1177:1200	several organic solvents	1177:1200	Among several organic solvents and mixtures, acetone 80% (v/v) was the most efficient for lignin solubilisation.
30703427	5	52	theme	important	923:931	arg1	influence					933:941	an important influence	920:941	an important influence	920:941	The results revealed that the process used for lignin isolation has an important influence in the final properties of the sample.
30703427	3	53	theme	structural	632:641	arg1	characteristics					661:675	the structural and morphological characteristics	628:675	the structural and morphological characteristics	628:675	Additionally, the structural and morphological characteristics, calorific value, thermal stability, solubility in solvents and antioxidant potential were also determined.
30703427	1	54	theme	lignin	157:162	arg1	samples					164:170	Four different lignin samples	142:170	Four different lignin samples	142:170	Four different lignin samples were isolated during the processing of rice straw for ethanol production: one after mild alkaline pretreatment (deacetylation) of rice straw, and other three after simultaneous saccharification and fermentation (fermentation residues), under different process conditions.
30703427	5	55	contain	has	916:918	arg2	influence					933:941	an important influence	920:941	an important influence	920:941	The results revealed that the process used for lignin isolation has an important influence in the final properties of the sample.
30703427	5	55	contain	has	916:918	arg1	process					882:888	the process	878:888	the process used for lignin isolation	878:914	The results revealed that the process used for lignin isolation has an important influence in the final properties of the sample.
30703427	8	56	theme	lignin	1353:1358	arg1	samples					1360:1366	all the lignin samples	1345:1366	all the lignin samples isolated from rice straw processing, which would allow their application in different industrial areas	1345:1469	Interesting properties and characteristics were observed for all the lignin samples isolated from rice straw processing, which would allow their application in different industrial areas.
30703427	6	57	theme	non-hydrolyzed	1030:1043	arg1	polysaccharides					1045:1059	non-hydrolyzed polysaccharides	1030:1059	non-hydrolyzed polysaccharides in their composition and lower lignin content	1030:1105	In general, the fermentation residues contained non-hydrolyzed polysaccharides in their composition and lower lignin content when compared to the lignin solubilized in the alkaline liquor.
30703427	3	58	from	value	688:692	arg1	solvents					728:735	solvents	728:735	solvents	728:735	Additionally, the structural and morphological characteristics, calorific value, thermal stability, solubility in solvents and antioxidant potential were also determined.
30703427	8	59	theme	industrial	1454:1463	arg1	areas					1465:1469	different industrial areas	1444:1469	different industrial areas	1444:1469	Interesting properties and characteristics were observed for all the lignin samples isolated from rice straw processing, which would allow their application in different industrial areas.
30703427	1	60	theme	mild	256:259	arg1	deacetylation					284:296	deacetylation	284:296	deacetylation	284:296	Four different lignin samples were isolated during the processing of rice straw for ethanol production: one after mild alkaline pretreatment (deacetylation) of rice straw, and other three after simultaneous saccharification and fermentation (fermentation residues), under different process conditions.
30703427	1	60	theme	mild	256:259	arg1	pretreatment					270:281	mild alkaline pretreatment	256:281	mild alkaline pretreatment (deacetylation) of rice straw	256:311	Four different lignin samples were isolated during the processing of rice straw for ethanol production: one after mild alkaline pretreatment (deacetylation) of rice straw, and other three after simultaneous saccharification and fermentation (fermentation residues), under different process conditions.
30703427	1	61	theme	simultaneous	336:347	arg1	saccharification					349:364	simultaneous saccharification	336:364	simultaneous saccharification	336:364	Four different lignin samples were isolated during the processing of rice straw for ethanol production: one after mild alkaline pretreatment (deacetylation) of rice straw, and other three after simultaneous saccharification and fermentation (fermentation residues), under different process conditions.
30703427	4	62	theme	Kraft	820:824	arg1	lignin					812:817	a standard lignin	801:817	a standard lignin (Kraft lignin)	801:832	For comparison, a standard lignin (Kraft lignin) was also assayed.
30703427	4	62	theme	Kraft	820:824	arg1	lignin					826:831	Kraft lignin	820:831	Kraft lignin	820:831	For comparison, a standard lignin (Kraft lignin) was also assayed.
30703427	3	63	from	characteristics	661:675	arg1	solvents					728:735	solvents	728:735	solvents	728:735	Additionally, the structural and morphological characteristics, calorific value, thermal stability, solubility in solvents and antioxidant potential were also determined.
30703427	5	64	theme	lignin	899:904	arg1	isolation					906:914	lignin isolation	899:914	lignin isolation	899:914	The results revealed that the process used for lignin isolation has an important influence in the final properties of the sample.
30703427	9	65	from	integration	1564:1574	arg1	biorefineries					1579:1591	biorefineries	1579:1591	biorefineries	1579:1591	Based on the properties, potential applications were suggested, which could be of value for integration in biorefineries.
30703427	1	66	theme	alkaline	261:268	arg1	deacetylation					284:296	deacetylation	284:296	deacetylation	284:296	Four different lignin samples were isolated during the processing of rice straw for ethanol production: one after mild alkaline pretreatment (deacetylation) of rice straw, and other three after simultaneous saccharification and fermentation (fermentation residues), under different process conditions.
30703427	1	66	theme	alkaline	261:268	arg1	pretreatment					270:281	mild alkaline pretreatment	256:281	mild alkaline pretreatment (deacetylation) of rice straw	256:311	Four different lignin samples were isolated during the processing of rice straw for ethanol production: one after mild alkaline pretreatment (deacetylation) of rice straw, and other three after simultaneous saccharification and fermentation (fermentation residues), under different process conditions.
29796085	7	0	theme	total	1170:1174	arg1	analysis					1188:1195	total composition analysis	1170:1195	total composition analysis (by NREL/TP-510-42618) of LCB prior to or after pretreatments	1170:1257	Comparison of bioconversion yield and total composition analysis (by NREL/TP-510-42618) of LCB prior to or after pretreatments did not show any close relationship.
29796085	4	1	theme	alkali	680:685	arg1	pretreatment					697:708	alkali extrusion pretreatment	680:708	alkali extrusion pretreatment	680:708	RESULTS Our results indicated that alkali extrusion pretreatment led to the highest hydrolysis rates for alfalfa stover, cattail stems and flax shives, despite its lower lignin removal efficiency compared to alkali pretreatment.
29796085	10	2	theme	hydrolysis	1755:1764	arg1	rates					1766:1770	enzymatic hydrolysis rates	1745:1770	enzymatic hydrolysis rates	1745:1770	The clear and robust relationships that were observed between the cellulose accessibility by FTCM probes and enzymatic hydrolysis rates change could be evolved into a powerful prediction tool that might help develop optimal biomass pretreatment strategies for biofuel production.
29796085	9	3	theme	key	1599:1601	arg1	determinant					1603:1613	the key determinant	1595:1613	the key determinant of hydrolysis yield	1595:1633	CONCLUSIONS Fluorescent protein-tagged carbohydrate-binding modules method (FTCM)-depletion assay provided direct evidence that cellulose exposure is the key determinant of hydrolysis yield.
29796085	9	3	theme	key	1599:1601	arg1	exposure					1583:1590	cellulose exposure	1573:1590	cellulose exposure	1573:1590	CONCLUSIONS Fluorescent protein-tagged carbohydrate-binding modules method (FTCM)-depletion assay provided direct evidence that cellulose exposure is the key determinant of hydrolysis yield.
29796085	8	4	theme	Lignin	1296:1301	arg1	efficiency					1311:1320	Lignin removal efficiency	1296:1320	Lignin removal efficiency	1296:1320	Lignin removal efficiency and total cellulose content (by NREL/TP-510-42618) led to an unreliable prediction of enzymatic polysaccharide hydrolysis.
29796085	6	5	theme	clear	976:980	arg1	relationship					982:993	A clear relationship	974:993	A clear relationship	974:993	A clear relationship was consistently observed between total surface-exposed cellulose detected by the FTCM-depletion assay and biomass enzymatic hydrolysis.
29796085	3	6	theme	enzymatic	616:624	arg1	hydrolysis					626:635	enzymatic hydrolysis	616:635	enzymatic hydrolysis of LCB	616:642	Here, we used fluorescent protein-tagged carbohydrate-binding modules method (FTCM)-depletion assay to study the relationship between surface-exposed polysaccharides and enzymatic hydrolysis of LCB.
29796085	5	7	theme	higher	949:954	arg1	rates					967:971	higher hydrolysis rates	949:971	higher hydrolysis rates	949:971	Corn crop residues were more sensitive to alkali pretreatments, leading to higher hydrolysis rates.
29796085	10	8	dep	accessibility	1712:1724	arg1	change					1772:1777	change	1772:1777	change	1772:1777	The clear and robust relationships that were observed between the cellulose accessibility by FTCM probes and enzymatic hydrolysis rates change could be evolved into a powerful prediction tool that might help develop optimal biomass pretreatment strategies for biofuel production.
29796085	9	9	theme	yield	1629:1633	arg1	determinant					1603:1613	the key determinant	1595:1613	the key determinant of hydrolysis yield	1595:1633	CONCLUSIONS Fluorescent protein-tagged carbohydrate-binding modules method (FTCM)-depletion assay provided direct evidence that cellulose exposure is the key determinant of hydrolysis yield.
29796085	9	9	theme	yield	1629:1633	arg1	exposure					1583:1590	cellulose exposure	1573:1590	cellulose exposure	1573:1590	CONCLUSIONS Fluorescent protein-tagged carbohydrate-binding modules method (FTCM)-depletion assay provided direct evidence that cellulose exposure is the key determinant of hydrolysis yield.
29796085	8	10	theme	total	1326:1330	arg1	content					1342:1348	total cellulose content	1326:1348	total cellulose content (by NREL/TP-510-42618)	1326:1371	Lignin removal efficiency and total cellulose content (by NREL/TP-510-42618) led to an unreliable prediction of enzymatic polysaccharide hydrolysis.
29796085	6	11	theme	FTCM-depletion	1077:1090	arg1	assay					1092:1096	the FTCM-depletion assay	1073:1096	the FTCM-depletion assay	1073:1096	A clear relationship was consistently observed between total surface-exposed cellulose detected by the FTCM-depletion assay and biomass enzymatic hydrolysis.
29796085	8	12	theme	unreliable	1383:1392	arg1	prediction					1394:1403	an unreliable prediction	1380:1403	an unreliable prediction of enzymatic polysaccharide hydrolysis	1380:1442	Lignin removal efficiency and total cellulose content (by NREL/TP-510-42618) led to an unreliable prediction of enzymatic polysaccharide hydrolysis.
29796085	2	13	theme	biomass	428:434	arg1	material					436:443	the biomass material	424:443	the biomass material	424:443	Determining the best pretreatment and its parameters requires monitoring its impacts on the biomass material.
29796085	10	14	theme	powerful	1803:1810	arg1	tool					1823:1826	a powerful prediction tool	1801:1826	a powerful prediction tool that might help develop optimal biomass pretreatment strategies for biofuel production	1801:1913	The clear and robust relationships that were observed between the cellulose accessibility by FTCM probes and enzymatic hydrolysis rates change could be evolved into a powerful prediction tool that might help develop optimal biomass pretreatment strategies for biofuel production.
29796085	0	15	theme	enzymatic	162:170	arg1	conversion					172:181	their enzymatic conversion	156:181	their enzymatic conversion using carbohydrate-binding modules	156:216	Determination of optimal biomass pretreatment strategies for biofuel production: investigation of relationships between surface-exposed polysaccharides and their enzymatic conversion using carbohydrate-binding modules.
29796085	7	16	theme	close	1276:1280	arg1	relationship					1282:1293	any close relationship	1272:1293	any close relationship	1272:1293	Comparison of bioconversion yield and total composition analysis (by NREL/TP-510-42618) of LCB prior to or after pretreatments did not show any close relationship.
29796085	8	17	theme	polysaccharide	1418:1431	arg1	hydrolysis					1433:1442	enzymatic polysaccharide hydrolysis	1408:1442	enzymatic polysaccharide hydrolysis	1408:1442	Lignin removal efficiency and total cellulose content (by NREL/TP-510-42618) led to an unreliable prediction of enzymatic polysaccharide hydrolysis.
29796085	7	18	dep	prior	1227:1231	arg1	to					1233:1234	to	1233:1234	to	1233:1234	Comparison of bioconversion yield and total composition analysis (by NREL/TP-510-42618) of LCB prior to or after pretreatments did not show any close relationship.
29796085	7	19	theme	composition	1176:1186	arg1	analysis					1188:1195	total composition analysis	1170:1195	total composition analysis (by NREL/TP-510-42618) of LCB prior to or after pretreatments	1170:1257	Comparison of bioconversion yield and total composition analysis (by NREL/TP-510-42618) of LCB prior to or after pretreatments did not show any close relationship.
29796085	6	20	theme	total	1029:1033	arg1	cellulose					1051:1059	total surface-exposed cellulose	1029:1059	total surface-exposed cellulose detected by the FTCM-depletion assay and biomass enzymatic hydrolysis	1029:1129	A clear relationship was consistently observed between total surface-exposed cellulose detected by the FTCM-depletion assay and biomass enzymatic hydrolysis.
29796085	4	21	theme	flax	784:787	arg1	shives					789:794	flax shives	784:794	flax shives	784:794	RESULTS Our results indicated that alkali extrusion pretreatment led to the highest hydrolysis rates for alfalfa stover, cattail stems and flax shives, despite its lower lignin removal efficiency compared to alkali pretreatment.
29796085	6	22	theme	enzymatic	1110:1118	arg1	hydrolysis					1120:1129	biomass enzymatic hydrolysis	1102:1129	biomass enzymatic hydrolysis	1102:1129	A clear relationship was consistently observed between total surface-exposed cellulose detected by the FTCM-depletion assay and biomass enzymatic hydrolysis.
29796085	0	23	theme	relationships	98:110	arg1	investigation					81:93	investigation	81:93	Determination of optimal biomass pretreatment strategies for biofuel production: investigation of relationships between surface-exposed polysaccharides and their enzymatic conversion using carbohydrate-binding modules.	0:217	Determination of optimal biomass pretreatment strategies for biofuel production: investigation of relationships between surface-exposed polysaccharides and their enzymatic conversion using carbohydrate-binding modules.
29796085	2	24	dep	pretreatment	357:368	arg1	the					348:350	the	348:350	the	348:350	Determining the best pretreatment and its parameters requires monitoring its impacts on the biomass material.
29796085	3	25	theme	-depletion	529:538	arg1	assay					540:544	fluorescent protein-tagged carbohydrate-binding modules method (FTCM)-depletion assay	460:544	fluorescent protein-tagged carbohydrate-binding modules method (FTCM)-depletion assay	460:544	Here, we used fluorescent protein-tagged carbohydrate-binding modules method (FTCM)-depletion assay to study the relationship between surface-exposed polysaccharides and enzymatic hydrolysis of LCB.
29796085	3	26	theme	modules	508:514	arg1	FTCM					524:527	FTCM	524:527	FTCM	524:527	Here, we used fluorescent protein-tagged carbohydrate-binding modules method (FTCM)-depletion assay to study the relationship between surface-exposed polysaccharides and enzymatic hydrolysis of LCB.
29796085	3	26	theme	modules	508:514	arg1	method					516:521	protein-tagged carbohydrate-binding modules method	472:521	fluorescent protein-tagged carbohydrate-binding modules method (FTCM)-depletion assay	460:544	Here, we used fluorescent protein-tagged carbohydrate-binding modules method (FTCM)-depletion assay to study the relationship between surface-exposed polysaccharides and enzymatic hydrolysis of LCB.
29796085	0	27	theme	surface-exposed	120:134	arg1	polysaccharides					136:150	surface-exposed polysaccharides	120:150	surface-exposed polysaccharides	120:150	Determination of optimal biomass pretreatment strategies for biofuel production: investigation of relationships between surface-exposed polysaccharides and their enzymatic conversion using carbohydrate-binding modules.
29796085	9	28	theme	protein-tagged	1469:1482	arg1	modules					1505:1511	Fluorescent protein-tagged carbohydrate-binding modules	1457:1511	CONCLUSIONS Fluorescent protein-tagged carbohydrate-binding modules method (FTCM)-depletion assay provided direct evidence that cellulose exposure is the key determinant of hydrolysis yield.	1445:1634	CONCLUSIONS Fluorescent protein-tagged carbohydrate-binding modules method (FTCM)-depletion assay provided direct evidence that cellulose exposure is the key determinant of hydrolysis yield.
29796085	4	29	theme	lignin	815:820	arg1	efficiency					830:839	its lower lignin removal efficiency	805:839	its lower lignin removal efficiency compared to alkali pretreatment	805:871	RESULTS Our results indicated that alkali extrusion pretreatment led to the highest hydrolysis rates for alfalfa stover, cattail stems and flax shives, despite its lower lignin removal efficiency compared to alkali pretreatment.
29796085	3	30	theme	protein-tagged	472:485	arg1	FTCM					524:527	FTCM	524:527	FTCM	524:527	Here, we used fluorescent protein-tagged carbohydrate-binding modules method (FTCM)-depletion assay to study the relationship between surface-exposed polysaccharides and enzymatic hydrolysis of LCB.
29796085	3	30	theme	protein-tagged	472:485	arg1	method					516:521	protein-tagged carbohydrate-binding modules method	472:521	fluorescent protein-tagged carbohydrate-binding modules method (FTCM)-depletion assay	460:544	Here, we used fluorescent protein-tagged carbohydrate-binding modules method (FTCM)-depletion assay to study the relationship between surface-exposed polysaccharides and enzymatic hydrolysis of LCB.
29796085	0	31	theme	optimal	17:23	arg1	strategies					46:55	optimal biomass pretreatment strategies	17:55	optimal biomass pretreatment strategies for biofuel production	17:78	Determination of optimal biomass pretreatment strategies for biofuel production: investigation of relationships between surface-exposed polysaccharides and their enzymatic conversion using carbohydrate-binding modules.
29796085	10	32	theme	optimal biomass	1852:1866	arg1	strategies					1881:1890	optimal biomass pretreatment strategies	1852:1890	optimal biomass pretreatment strategies for biofuel production	1852:1913	The clear and robust relationships that were observed between the cellulose accessibility by FTCM probes and enzymatic hydrolysis rates change could be evolved into a powerful prediction tool that might help develop optimal biomass pretreatment strategies for biofuel production.
29796085	0	33	theme	pretreatment	33:44	arg1	strategies					46:55	optimal biomass pretreatment strategies	17:55	optimal biomass pretreatment strategies for biofuel production	17:78	Determination of optimal biomass pretreatment strategies for biofuel production: investigation of relationships between surface-exposed polysaccharides and their enzymatic conversion using carbohydrate-binding modules.
29796085	5	34	theme	Corn	874:877	arg1	residues					884:891	Corn crop residues	874:891	Corn crop residues	874:891	Corn crop residues were more sensitive to alkali pretreatments, leading to higher hydrolysis rates.
29796085	10	35	theme	cellulose	1702:1710	arg1	accessibility					1712:1724	the cellulose accessibility	1698:1724	the cellulose accessibility by FTCM probes	1698:1739	The clear and robust relationships that were observed between the cellulose accessibility by FTCM probes and enzymatic hydrolysis rates change could be evolved into a powerful prediction tool that might help develop optimal biomass pretreatment strategies for biofuel production.
29796085	10	36	theme	biofuel	1896:1902	arg1	production					1904:1913	biofuel production	1896:1913	biofuel production	1896:1913	The clear and robust relationships that were observed between the cellulose accessibility by FTCM probes and enzymatic hydrolysis rates change could be evolved into a powerful prediction tool that might help develop optimal biomass pretreatment strategies for biofuel production.
29796085	9	37	theme	direct	1552:1557	arg1	evidence					1559:1566	direct evidence that cellulose exposure is the key determinant of hydrolysis yield	1552:1633	direct evidence that cellulose exposure is the key determinant of hydrolysis yield	1552:1633	CONCLUSIONS Fluorescent protein-tagged carbohydrate-binding modules method (FTCM)-depletion assay provided direct evidence that cellulose exposure is the key determinant of hydrolysis yield.
29796085	3	38	used	used	455:458	arg2	we					452:453	we	452:453	we	452:453	Here, we used fluorescent protein-tagged carbohydrate-binding modules method (FTCM)-depletion assay to study the relationship between surface-exposed polysaccharides and enzymatic hydrolysis of LCB.
29796085	4	39	theme	extrusion	687:695	arg1	pretreatment					697:708	alkali extrusion pretreatment	680:708	alkali extrusion pretreatment	680:708	RESULTS Our results indicated that alkali extrusion pretreatment led to the highest hydrolysis rates for alfalfa stover, cattail stems and flax shives, despite its lower lignin removal efficiency compared to alkali pretreatment.
29796085	3	40	theme	surface-exposed	580:594	arg1	polysaccharides					596:610	surface-exposed polysaccharides	580:610	surface-exposed polysaccharides	580:610	Here, we used fluorescent protein-tagged carbohydrate-binding modules method (FTCM)-depletion assay to study the relationship between surface-exposed polysaccharides and enzymatic hydrolysis of LCB.
29796085	1	41	theme	key	281:283	arg1	step					285:288	a key step	279:288	a key step for its efficient bioconversion into ethanol	279:333	BACKGROUND Pretreatment of lignocellulosic biomass (LCB) is a key step for its efficient bioconversion into ethanol.
29796085	1	41	theme	key	281:283	arg1	Pretreatment					230:241	BACKGROUND Pretreatment	219:241	BACKGROUND Pretreatment of lignocellulosic biomass (LCB)	219:274	BACKGROUND Pretreatment of lignocellulosic biomass (LCB) is a key step for its efficient bioconversion into ethanol.
29796085	0	42	theme	biofuel	61:67	arg1	production					69:78	biofuel production	61:78	biofuel production	61:78	Determination of optimal biomass pretreatment strategies for biofuel production: investigation of relationships between surface-exposed polysaccharides and their enzymatic conversion using carbohydrate-binding modules.
29796085	4	43	theme	highest	721:727	arg1	rates					740:744	the highest hydrolysis rates	717:744	the highest hydrolysis rates for alfalfa stover, cattail stems and flax shives	717:794	RESULTS Our results indicated that alkali extrusion pretreatment led to the highest hydrolysis rates for alfalfa stover, cattail stems and flax shives, despite its lower lignin removal efficiency compared to alkali pretreatment.
29796085	1	44	theme	efficient	298:306	arg1	bioconversion					308:320	its efficient bioconversion	294:320	its efficient bioconversion into ethanol	294:333	BACKGROUND Pretreatment of lignocellulosic biomass (LCB) is a key step for its efficient bioconversion into ethanol.
29796085	10	45	theme	enzymatic	1745:1753	arg1	rates					1766:1770	enzymatic hydrolysis rates	1745:1770	enzymatic hydrolysis rates	1745:1770	The clear and robust relationships that were observed between the cellulose accessibility by FTCM probes and enzymatic hydrolysis rates change could be evolved into a powerful prediction tool that might help develop optimal biomass pretreatment strategies for biofuel production.
29796085	7	46	theme	LCB	1223:1225	arg1	Comparison					1132:1141	Comparison	1132:1141	Comparison of bioconversion yield	1132:1164	Comparison of bioconversion yield and total composition analysis (by NREL/TP-510-42618) of LCB prior to or after pretreatments did not show any close relationship.
29796085	7	46	theme	LCB	1223:1225	arg1	analysis					1188:1195	total composition analysis	1170:1195	total composition analysis (by NREL/TP-510-42618) of LCB prior to or after pretreatments	1170:1257	Comparison of bioconversion yield and total composition analysis (by NREL/TP-510-42618) of LCB prior to or after pretreatments did not show any close relationship.
29796085	9	47	theme	hydrolysis	1618:1627	arg1	yield					1629:1633	hydrolysis yield	1618:1633	hydrolysis yield	1618:1633	CONCLUSIONS Fluorescent protein-tagged carbohydrate-binding modules method (FTCM)-depletion assay provided direct evidence that cellulose exposure is the key determinant of hydrolysis yield.
29796085	1	48	theme	BACKGROUND	219:228	arg1	step					285:288	a key step	279:288	a key step for its efficient bioconversion into ethanol	279:333	BACKGROUND Pretreatment of lignocellulosic biomass (LCB) is a key step for its efficient bioconversion into ethanol.
29796085	1	48	theme	BACKGROUND	219:228	arg1	Pretreatment					230:241	BACKGROUND Pretreatment	219:241	BACKGROUND Pretreatment of lignocellulosic biomass (LCB)	219:274	BACKGROUND Pretreatment of lignocellulosic biomass (LCB) is a key step for its efficient bioconversion into ethanol.
29796085	2	49	from	impacts	413:419	arg1	material					436:443	the biomass material	424:443	the biomass material	424:443	Determining the best pretreatment and its parameters requires monitoring its impacts on the biomass material.
29796085	5	50	theme	hydrolysis	956:965	arg1	rates					967:971	higher hydrolysis rates	949:971	higher hydrolysis rates	949:971	Corn crop residues were more sensitive to alkali pretreatments, leading to higher hydrolysis rates.
29796085	8	51	theme	removal	1303:1309	arg1	efficiency					1311:1320	Lignin removal efficiency	1296:1320	Lignin removal efficiency	1296:1320	Lignin removal efficiency and total cellulose content (by NREL/TP-510-42618) led to an unreliable prediction of enzymatic polysaccharide hydrolysis.
29796085	2	52	theme	best	352:355	arg1	pretreatment					357:368	pretreatment	357:368	pretreatment	357:368	Determining the best pretreatment and its parameters requires monitoring its impacts on the biomass material.
29796085	5	53	theme	alkali	916:921	arg1	pretreatments					923:935	alkali pretreatments	916:935	alkali pretreatments	916:935	Corn crop residues were more sensitive to alkali pretreatments, leading to higher hydrolysis rates.
29796085	10	54	theme	prediction	1812:1821	arg1	tool					1823:1826	a powerful prediction tool	1801:1826	a powerful prediction tool that might help develop optimal biomass pretreatment strategies for biofuel production	1801:1913	The clear and robust relationships that were observed between the cellulose accessibility by FTCM probes and enzymatic hydrolysis rates change could be evolved into a powerful prediction tool that might help develop optimal biomass pretreatment strategies for biofuel production.
29796085	1	55	theme	lignocellulosic	246:260	arg1	LCB					271:273	LCB	271:273	LCB	271:273	BACKGROUND Pretreatment of lignocellulosic biomass (LCB) is a key step for its efficient bioconversion into ethanol.
29796085	1	55	theme	lignocellulosic	246:260	arg1	biomass					262:268	lignocellulosic biomass	246:268	lignocellulosic biomass (LCB)	246:274	BACKGROUND Pretreatment of lignocellulosic biomass (LCB) is a key step for its efficient bioconversion into ethanol.
29796085	8	56	theme	cellulose	1332:1340	arg1	content					1342:1348	total cellulose content	1326:1348	total cellulose content (by NREL/TP-510-42618)	1326:1371	Lignin removal efficiency and total cellulose content (by NREL/TP-510-42618) led to an unreliable prediction of enzymatic polysaccharide hydrolysis.
29796085	4	57	theme	hydrolysis	729:738	arg1	rates					740:744	the highest hydrolysis rates	717:744	the highest hydrolysis rates for alfalfa stover, cattail stems and flax shives	717:794	RESULTS Our results indicated that alkali extrusion pretreatment led to the highest hydrolysis rates for alfalfa stover, cattail stems and flax shives, despite its lower lignin removal efficiency compared to alkali pretreatment.
29796085	0	58	theme	carbohydrate-binding	189:208	arg1	modules					210:216	carbohydrate-binding modules	189:216	carbohydrate-binding modules	189:216	Determination of optimal biomass pretreatment strategies for biofuel production: investigation of relationships between surface-exposed polysaccharides and their enzymatic conversion using carbohydrate-binding modules.
29796085	7	59	theme	bioconversion	1146:1158	arg1	yield					1160:1164	bioconversion yield	1146:1164	bioconversion yield	1146:1164	Comparison of bioconversion yield and total composition analysis (by NREL/TP-510-42618) of LCB prior to or after pretreatments did not show any close relationship.
29796085	6	60	theme	surface-exposed	1035:1049	arg1	cellulose					1051:1059	total surface-exposed cellulose	1029:1059	total surface-exposed cellulose detected by the FTCM-depletion assay and biomass enzymatic hydrolysis	1029:1129	A clear relationship was consistently observed between total surface-exposed cellulose detected by the FTCM-depletion assay and biomass enzymatic hydrolysis.
29796085	8	61	theme	enzymatic	1408:1416	arg1	hydrolysis					1433:1442	enzymatic polysaccharide hydrolysis	1408:1442	enzymatic polysaccharide hydrolysis	1408:1442	Lignin removal efficiency and total cellulose content (by NREL/TP-510-42618) led to an unreliable prediction of enzymatic polysaccharide hydrolysis.
29796085	7	62	theme	yield	1160:1164	arg1	Comparison					1132:1141	Comparison	1132:1141	Comparison of bioconversion yield	1132:1164	Comparison of bioconversion yield and total composition analysis (by NREL/TP-510-42618) of LCB prior to or after pretreatments did not show any close relationship.
29796085	7	62	theme	yield	1160:1164	arg1	analysis					1188:1195	total composition analysis	1170:1195	total composition analysis (by NREL/TP-510-42618) of LCB prior to or after pretreatments	1170:1257	Comparison of bioconversion yield and total composition analysis (by NREL/TP-510-42618) of LCB prior to or after pretreatments did not show any close relationship.
29796085	3	63	theme	LCB	640:642	arg1	hydrolysis					626:635	enzymatic hydrolysis	616:635	enzymatic hydrolysis of LCB	616:642	Here, we used fluorescent protein-tagged carbohydrate-binding modules method (FTCM)-depletion assay to study the relationship between surface-exposed polysaccharides and enzymatic hydrolysis of LCB.
29796085	3	63	theme	LCB	640:642	arg1	polysaccharides					596:610	surface-exposed polysaccharides	580:610	surface-exposed polysaccharides	580:610	Here, we used fluorescent protein-tagged carbohydrate-binding modules method (FTCM)-depletion assay to study the relationship between surface-exposed polysaccharides and enzymatic hydrolysis of LCB.
29796085	8	64	theme	hydrolysis	1433:1442	arg1	prediction					1394:1403	an unreliable prediction	1380:1403	an unreliable prediction of enzymatic polysaccharide hydrolysis	1380:1442	Lignin removal efficiency and total cellulose content (by NREL/TP-510-42618) led to an unreliable prediction of enzymatic polysaccharide hydrolysis.
29796085	1	65	theme	biomass	262:268	arg1	step					285:288	a key step	279:288	a key step for its efficient bioconversion into ethanol	279:333	BACKGROUND Pretreatment of lignocellulosic biomass (LCB) is a key step for its efficient bioconversion into ethanol.
29796085	1	65	theme	biomass	262:268	arg1	Pretreatment					230:241	BACKGROUND Pretreatment	219:241	BACKGROUND Pretreatment of lignocellulosic biomass (LCB)	219:274	BACKGROUND Pretreatment of lignocellulosic biomass (LCB) is a key step for its efficient bioconversion into ethanol.
29796085	4	66	theme	cattail	766:772	arg1	stems					774:778	cattail stems	766:778	cattail stems	766:778	RESULTS Our results indicated that alkali extrusion pretreatment led to the highest hydrolysis rates for alfalfa stover, cattail stems and flax shives, despite its lower lignin removal efficiency compared to alkali pretreatment.
29796085	6	67	theme	biomass	1102:1108	arg1	hydrolysis					1120:1129	biomass enzymatic hydrolysis	1102:1129	biomass enzymatic hydrolysis	1102:1129	A clear relationship was consistently observed between total surface-exposed cellulose detected by the FTCM-depletion assay and biomass enzymatic hydrolysis.
29796085	4	68	dep	RESULTS	645:651	arg1	indicated					665:673	indicated	665:673	indicated that alkali extrusion pretreatment led to the highest hydrolysis rates for alfalfa stover, cattail stems and flax shives, despite its lower lignin removal efficiency compared to alkali pretreatment	665:871	RESULTS Our results indicated that alkali extrusion pretreatment led to the highest hydrolysis rates for alfalfa stover, cattail stems and flax shives, despite its lower lignin removal efficiency compared to alkali pretreatment.
29796085	10	69	theme	clear	1640:1644	arg1	relationships					1657:1669	The clear and robust relationships	1636:1669	The clear and robust relationships that were observed between the cellulose accessibility by FTCM probes and enzymatic hydrolysis rates change	1636:1777	The clear and robust relationships that were observed between the cellulose accessibility by FTCM probes and enzymatic hydrolysis rates change could be evolved into a powerful prediction tool that might help develop optimal biomass pretreatment strategies for biofuel production.
29796085	4	70	theme	alkali	853:858	arg1	pretreatment					860:871	alkali pretreatment	853:871	alkali pretreatment	853:871	RESULTS Our results indicated that alkali extrusion pretreatment led to the highest hydrolysis rates for alfalfa stover, cattail stems and flax shives, despite its lower lignin removal efficiency compared to alkali pretreatment.
29796085	3	71	theme	method	516:521	arg1	assay					540:544	fluorescent protein-tagged carbohydrate-binding modules method (FTCM)-depletion assay	460:544	fluorescent protein-tagged carbohydrate-binding modules method (FTCM)-depletion assay	460:544	Here, we used fluorescent protein-tagged carbohydrate-binding modules method (FTCM)-depletion assay to study the relationship between surface-exposed polysaccharides and enzymatic hydrolysis of LCB.
29796085	9	72	theme	carbohydrate-binding	1484:1503	arg1	modules					1505:1511	Fluorescent protein-tagged carbohydrate-binding modules	1457:1511	CONCLUSIONS Fluorescent protein-tagged carbohydrate-binding modules method (FTCM)-depletion assay provided direct evidence that cellulose exposure is the key determinant of hydrolysis yield.	1445:1634	CONCLUSIONS Fluorescent protein-tagged carbohydrate-binding modules method (FTCM)-depletion assay provided direct evidence that cellulose exposure is the key determinant of hydrolysis yield.
29796085	9	73	theme	Fluorescent	1457:1467	arg1	modules					1505:1511	Fluorescent protein-tagged carbohydrate-binding modules	1457:1511	CONCLUSIONS Fluorescent protein-tagged carbohydrate-binding modules method (FTCM)-depletion assay provided direct evidence that cellulose exposure is the key determinant of hydrolysis yield.	1445:1634	CONCLUSIONS Fluorescent protein-tagged carbohydrate-binding modules method (FTCM)-depletion assay provided direct evidence that cellulose exposure is the key determinant of hydrolysis yield.
29796085	4	74	theme	lower	809:813	arg1	efficiency					830:839	its lower lignin removal efficiency	805:839	its lower lignin removal efficiency compared to alkali pretreatment	805:871	RESULTS Our results indicated that alkali extrusion pretreatment led to the highest hydrolysis rates for alfalfa stover, cattail stems and flax shives, despite its lower lignin removal efficiency compared to alkali pretreatment.
29796085	3	75	theme	carbohydrate-binding	487:506	arg1	FTCM					524:527	FTCM	524:527	FTCM	524:527	Here, we used fluorescent protein-tagged carbohydrate-binding modules method (FTCM)-depletion assay to study the relationship between surface-exposed polysaccharides and enzymatic hydrolysis of LCB.
29796085	3	75	theme	carbohydrate-binding	487:506	arg1	method					516:521	protein-tagged carbohydrate-binding modules method	472:521	fluorescent protein-tagged carbohydrate-binding modules method (FTCM)-depletion assay	460:544	Here, we used fluorescent protein-tagged carbohydrate-binding modules method (FTCM)-depletion assay to study the relationship between surface-exposed polysaccharides and enzymatic hydrolysis of LCB.
29796085	0	76	theme	biomass	25:31	arg1	strategies					46:55	optimal biomass pretreatment strategies	17:55	optimal biomass pretreatment strategies for biofuel production	17:78	Determination of optimal biomass pretreatment strategies for biofuel production: investigation of relationships between surface-exposed polysaccharides and their enzymatic conversion using carbohydrate-binding modules.
29796085	4	77	theme	removal	822:828	arg1	efficiency					830:839	its lower lignin removal efficiency	805:839	its lower lignin removal efficiency compared to alkali pretreatment	805:871	RESULTS Our results indicated that alkali extrusion pretreatment led to the highest hydrolysis rates for alfalfa stover, cattail stems and flax shives, despite its lower lignin removal efficiency compared to alkali pretreatment.
29796085	10	78	theme	pretreatment	1868:1879	arg1	strategies					1881:1890	optimal biomass pretreatment strategies	1852:1890	optimal biomass pretreatment strategies for biofuel production	1852:1913	The clear and robust relationships that were observed between the cellulose accessibility by FTCM probes and enzymatic hydrolysis rates change could be evolved into a powerful prediction tool that might help develop optimal biomass pretreatment strategies for biofuel production.
29796085	0	79	theme	strategies	46:55	arg1	Determination					0:12	Determination	0:12	Determination of optimal biomass pretreatment strategies for biofuel production: investigation of relationships between surface-exposed polysaccharides and their enzymatic conversion using carbohydrate-binding modules.	0:217	Determination of optimal biomass pretreatment strategies for biofuel production: investigation of relationships between surface-exposed polysaccharides and their enzymatic conversion using carbohydrate-binding modules.
29796085	3	80	theme	fluorescent	460:470	arg1	assay					540:544	fluorescent protein-tagged carbohydrate-binding modules method (FTCM)-depletion assay	460:544	fluorescent protein-tagged carbohydrate-binding modules method (FTCM)-depletion assay	460:544	Here, we used fluorescent protein-tagged carbohydrate-binding modules method (FTCM)-depletion assay to study the relationship between surface-exposed polysaccharides and enzymatic hydrolysis of LCB.
29796085	10	81	theme	robust	1650:1655	arg1	relationships					1657:1669	The clear and robust relationships	1636:1669	The clear and robust relationships that were observed between the cellulose accessibility by FTCM probes and enzymatic hydrolysis rates change	1636:1777	The clear and robust relationships that were observed between the cellulose accessibility by FTCM probes and enzymatic hydrolysis rates change could be evolved into a powerful prediction tool that might help develop optimal biomass pretreatment strategies for biofuel production.
29796085	5	82	theme	crop	879:882	arg1	residues					884:891	Corn crop residues	874:891	Corn crop residues	874:891	Corn crop residues were more sensitive to alkali pretreatments, leading to higher hydrolysis rates.
29796085	9	83	theme	-depletion	1526:1535	arg1	assay					1537:1541	(FTCM)-depletion assay	1520:1541	(FTCM)-depletion assay	1520:1541	CONCLUSIONS Fluorescent protein-tagged carbohydrate-binding modules method (FTCM)-depletion assay provided direct evidence that cellulose exposure is the key determinant of hydrolysis yield.
29796085	9	84	dep	CONCLUSIONS	1445:1455	arg1	modules					1505:1511	Fluorescent protein-tagged carbohydrate-binding modules	1457:1511	CONCLUSIONS Fluorescent protein-tagged carbohydrate-binding modules method (FTCM)-depletion assay provided direct evidence that cellulose exposure is the key determinant of hydrolysis yield.	1445:1634	CONCLUSIONS Fluorescent protein-tagged carbohydrate-binding modules method (FTCM)-depletion assay provided direct evidence that cellulose exposure is the key determinant of hydrolysis yield.
29796085	9	84	dep	CONCLUSIONS	1445:1455	arg1	provided					1543:1550	provided	1543:1550	provided direct evidence that cellulose exposure is the key determinant of hydrolysis yield	1543:1633	CONCLUSIONS Fluorescent protein-tagged carbohydrate-binding modules method (FTCM)-depletion assay provided direct evidence that cellulose exposure is the key determinant of hydrolysis yield.
29796085	0	85	dep	Determination	0:12	arg1	investigation					81:93	investigation	81:93	Determination of optimal biomass pretreatment strategies for biofuel production: investigation of relationships between surface-exposed polysaccharides and their enzymatic conversion using carbohydrate-binding modules.	0:217	Determination of optimal biomass pretreatment strategies for biofuel production: investigation of relationships between surface-exposed polysaccharides and their enzymatic conversion using carbohydrate-binding modules.
29796085	10	86	theme	FTCM	1729:1732	arg1	probes					1734:1739	FTCM probes	1729:1739	FTCM probes	1729:1739	The clear and robust relationships that were observed between the cellulose accessibility by FTCM probes and enzymatic hydrolysis rates change could be evolved into a powerful prediction tool that might help develop optimal biomass pretreatment strategies for biofuel production.
29796085	9	87	theme	cellulose	1573:1581	arg1	determinant					1603:1613	the key determinant	1595:1613	the key determinant of hydrolysis yield	1595:1633	CONCLUSIONS Fluorescent protein-tagged carbohydrate-binding modules method (FTCM)-depletion assay provided direct evidence that cellulose exposure is the key determinant of hydrolysis yield.
29796085	9	87	theme	cellulose	1573:1581	arg1	exposure					1583:1590	cellulose exposure	1573:1590	cellulose exposure	1573:1590	CONCLUSIONS Fluorescent protein-tagged carbohydrate-binding modules method (FTCM)-depletion assay provided direct evidence that cellulose exposure is the key determinant of hydrolysis yield.
30037904	6	0	theme	disease	1146:1152	arg1	state					1154:1158	the disease state	1142:1158	the disease state	1142:1158	To examine the role of mutual regulation of OGT and OGA in the disease state, we analyzed gene expression data from human cancer data sets, which revealed that OGT and OGA expression levels are highly correlated in numerous human cancers, particularly in pancreatic adenocarcinoma.
30037904	8	1	theme	transcriptional	1747:1761	arg1	mechanism					1763:1771	a transcriptional mechanism	1745:1771	a transcriptional mechanism that regulates cellular O-GlcNAc homeostasis, which may lay a foundation for exploring O-GlcNAc signaling as a therapeutic target for human disease	1745:1919	Our study elucidates a transcriptional mechanism that regulates cellular O-GlcNAc homeostasis, which may lay a foundation for exploring O-GlcNAc signaling as a therapeutic target for human disease.
30037904	1	2	theme	environmental	284:296	arg1	cues					298:301	diverse environmental cues	276:301	diverse environmental cues	276:301	Many intracellular proteins are reversibly modified by O-linked GlcNAc (O-GlcNAc), a post-translational modification that dynamically regulates fundamental cellular processes in response to diverse environmental cues.
30037904	6	3	theme	expression	1255:1264	arg1	levels					1266:1271	OGT and OGA expression levels	1243:1271	OGT and OGA expression levels	1243:1271	To examine the role of mutual regulation of OGT and OGA in the disease state, we analyzed gene expression data from human cancer data sets, which revealed that OGT and OGA expression levels are highly correlated in numerous human cancers, particularly in pancreatic adenocarcinoma.
30037904	5	4	theme	acetyltransferase	992:1008	arg1	p300					1010:1013	the histone acetyltransferase p300	980:1013	the histone acetyltransferase p300	980:1013	Specifically, we found that OGA promotes Ogt transcription through cooperation with the histone acetyltransferase p300 and transcription factor CCAAT/enhancer-binding protein β (C/EBPβ).
30037904	6	5	theme	human	1307:1311	arg1	cancers					1313:1319	numerous human cancers	1298:1319	numerous human cancers	1298:1319	To examine the role of mutual regulation of OGT and OGA in the disease state, we analyzed gene expression data from human cancer data sets, which revealed that OGT and OGA expression levels are highly correlated in numerous human cancers, particularly in pancreatic adenocarcinoma.
30037904	2	6	theme	Accumulating	304:315	arg1	evidence					317:324	Accumulating evidence	304:324	Accumulating evidence	304:324	Accumulating evidence indicates that both excess and deficiency of protein O-GlcNAcylation can have deleterious effects on the cell, suggesting that maintenance of O-GlcNAc homeostasis is essential for proper cellular function.
30037904	8	7	theme	cellular	1788:1795	arg1	homeostasis					1806:1816	cellular O-GlcNAc homeostasis	1788:1816	cellular O-GlcNAc homeostasis	1788:1816	Our study elucidates a transcriptional mechanism that regulates cellular O-GlcNAc homeostasis, which may lay a foundation for exploring O-GlcNAc signaling as a therapeutic target for human disease.
30037904	1	8	theme	post-translational	171:188	arg1	modification					190:201	a post-translational modification	169:201	a post-translational modification that dynamically regulates fundamental cellular processes in response to diverse environmental cues	169:301	Many intracellular proteins are reversibly modified by O-linked GlcNAc (O-GlcNAc), a post-translational modification that dynamically regulates fundamental cellular processes in response to diverse environmental cues.
30037904	1	8	theme	post-translational	171:188	arg1	GlcNAc					150:155	O-linked GlcNAc	141:155	O-linked GlcNAc (O-GlcNAc)	141:166	Many intracellular proteins are reversibly modified by O-linked GlcNAc (O-GlcNAc), a post-translational modification that dynamically regulates fundamental cellular processes in response to diverse environmental cues.
30037904	7	9	theme	signal-regulated	1493:1508	arg1	ERK					1518:1520	extracellular signal-regulated kinase (ERK)	1479:1521	extracellular signal-regulated kinase (ERK) signaling	1479:1531	Using a Kras -driven primary mouse pancreatic ductal adenocarcinoma (PDAC) cell line, we found that inhibition of extracellular signal-regulated kinase (ERK) signaling decreases OGA glycosidase activity and reduces OGT mRNA and protein levels, suggesting that ERK signaling may alter O-GlcNAc homeostasis in PDAC by modulating OGA-mediated Ogt transcription.
30037904	6	10	theme	cancer	1205:1210	arg1	sets					1217:1220	human cancer data sets	1199:1220	human cancer data sets	1199:1220	To examine the role of mutual regulation of OGT and OGA in the disease state, we analyzed gene expression data from human cancer data sets, which revealed that OGT and OGA expression levels are highly correlated in numerous human cancers, particularly in pancreatic adenocarcinoma.
30037904	7	11	theme	OGA-mediated	1692:1703	arg1	transcription					1709:1721	OGA-mediated Ogt transcription	1692:1721	OGA-mediated Ogt transcription	1692:1721	Using a Kras -driven primary mouse pancreatic ductal adenocarcinoma (PDAC) cell line, we found that inhibition of extracellular signal-regulated kinase (ERK) signaling decreases OGA glycosidase activity and reduces OGT mRNA and protein levels, suggesting that ERK signaling may alter O-GlcNAc homeostasis in PDAC by modulating OGA-mediated Ogt transcription.
30037904	7	12	theme	cell	1440:1443	arg1	line					1445:1448	a Kras -driven primary mouse pancreatic ductal adenocarcinoma (PDAC) cell line	1371:1448	a Kras -driven primary mouse pancreatic ductal adenocarcinoma (PDAC) cell line	1371:1448	Using a Kras -driven primary mouse pancreatic ductal adenocarcinoma (PDAC) cell line, we found that inhibition of extracellular signal-regulated kinase (ERK) signaling decreases OGA glycosidase activity and reduces OGT mRNA and protein levels, suggesting that ERK signaling may alter O-GlcNAc homeostasis in PDAC by modulating OGA-mediated Ogt transcription.
30037904	6	13	theme	OGT	1127:1129	arg1	regulation					1113:1122	mutual regulation	1106:1122	mutual regulation of OGT and OGA	1106:1137	To examine the role of mutual regulation of OGT and OGA in the disease state, we analyzed gene expression data from human cancer data sets, which revealed that OGT and OGA expression levels are highly correlated in numerous human cancers, particularly in pancreatic adenocarcinoma.
30037904	1	14	theme	cellular	242:249	arg1	processes					251:259	fundamental cellular processes	230:259	fundamental cellular processes	230:259	Many intracellular proteins are reversibly modified by O-linked GlcNAc (O-GlcNAc), a post-translational modification that dynamically regulates fundamental cellular processes in response to diverse environmental cues.
30037904	7	15	theme	adenocarcinoma	1418:1431	arg1	line					1445:1448	a Kras -driven primary mouse pancreatic ductal adenocarcinoma (PDAC) cell line	1371:1448	a Kras -driven primary mouse pancreatic ductal adenocarcinoma (PDAC) cell line	1371:1448	Using a Kras -driven primary mouse pancreatic ductal adenocarcinoma (PDAC) cell line, we found that inhibition of extracellular signal-regulated kinase (ERK) signaling decreases OGA glycosidase activity and reduces OGT mRNA and protein levels, suggesting that ERK signaling may alter O-GlcNAc homeostasis in PDAC by modulating OGA-mediated Ogt transcription.
30037904	4	16	theme	mutual	767:772	arg1	regulation					774:783	mutual regulation	767:783	mutual regulation of the O-GlcNAc-cycling enzymes O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA)	767:864	Here, we demonstrate the existence of a homeostatic mechanism involving mutual regulation of the O-GlcNAc-cycling enzymes O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA) at the transcriptional level.
30037904	4	17	dep	enzymes	809:815	arg1	enzymes					809:815	the O-GlcNAc-cycling enzymes	788:815	the O-GlcNAc-cycling enzymes O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA)	788:864	Here, we demonstrate the existence of a homeostatic mechanism involving mutual regulation of the O-GlcNAc-cycling enzymes O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA) at the transcriptional level.
30037904	4	17	dep	enzymes	809:815	arg1	OGA					861:863	OGA	861:863	OGA	861:863	Here, we demonstrate the existence of a homeostatic mechanism involving mutual regulation of the O-GlcNAc-cycling enzymes O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA) at the transcriptional level.
30037904	4	17	dep	enzymes	809:815	arg1	OGT					839:841	OGT	839:841	OGT	839:841	Here, we demonstrate the existence of a homeostatic mechanism involving mutual regulation of the O-GlcNAc-cycling enzymes O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA) at the transcriptional level.
30037904	4	17	dep	enzymes	809:815	arg1	O-GlcNAcase					848:858	O-GlcNAcase	848:858	O-GlcNAcase (OGA)	848:864	Here, we demonstrate the existence of a homeostatic mechanism involving mutual regulation of the O-GlcNAc-cycling enzymes O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA) at the transcriptional level.
30037904	4	17	dep	enzymes	809:815	arg1	transferase					826:836	O-GlcNAc transferase	817:836	O-GlcNAc transferase (OGT)	817:842	Here, we demonstrate the existence of a homeostatic mechanism involving mutual regulation of the O-GlcNAc-cycling enzymes O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA) at the transcriptional level.
30037904	7	18	theme	O-GlcNAc	1649:1656	arg1	homeostasis					1658:1668	O-GlcNAc homeostasis	1649:1668	O-GlcNAc homeostasis in PDAC	1649:1676	Using a Kras -driven primary mouse pancreatic ductal adenocarcinoma (PDAC) cell line, we found that inhibition of extracellular signal-regulated kinase (ERK) signaling decreases OGA glycosidase activity and reduces OGT mRNA and protein levels, suggesting that ERK signaling may alter O-GlcNAc homeostasis in PDAC by modulating OGA-mediated Ogt transcription.
30037904	7	19	theme	pancreatic	1400:1409	arg1	PDAC					1434:1437	PDAC	1434:1437	PDAC	1434:1437	Using a Kras -driven primary mouse pancreatic ductal adenocarcinoma (PDAC) cell line, we found that inhibition of extracellular signal-regulated kinase (ERK) signaling decreases OGA glycosidase activity and reduces OGT mRNA and protein levels, suggesting that ERK signaling may alter O-GlcNAc homeostasis in PDAC by modulating OGA-mediated Ogt transcription.
30037904	7	19	theme	pancreatic	1400:1409	arg1	adenocarcinoma					1418:1431	a Kras -driven primary mouse pancreatic ductal adenocarcinoma	1371:1431	a Kras -driven primary mouse pancreatic ductal adenocarcinoma (PDAC) cell line	1371:1448	Using a Kras -driven primary mouse pancreatic ductal adenocarcinoma (PDAC) cell line, we found that inhibition of extracellular signal-regulated kinase (ERK) signaling decreases OGA glycosidase activity and reduces OGT mRNA and protein levels, suggesting that ERK signaling may alter O-GlcNAc homeostasis in PDAC by modulating OGA-mediated Ogt transcription.
30037904	5	20	dep	factor	1033:1038	arg1	protein					1063:1069	CCAAT/enhancer-binding protein β	1040:1071	transcription factor CCAAT/enhancer-binding protein β (C/EBPβ)	1019:1080	Specifically, we found that OGA promotes Ogt transcription through cooperation with the histone acetyltransferase p300 and transcription factor CCAAT/enhancer-binding protein β (C/EBPβ).
30037904	5	20	dep	factor	1033:1038	arg1	C/EBPβ					1074:1079	C/EBPβ	1074:1079	C/EBPβ	1074:1079	Specifically, we found that OGA promotes Ogt transcription through cooperation with the histone acetyltransferase p300 and transcription factor CCAAT/enhancer-binding protein β (C/EBPβ).
30037904	5	21	theme	histone	984:990	arg1	p300					1010:1013	the histone acetyltransferase p300	980:1013	the histone acetyltransferase p300	980:1013	Specifically, we found that OGA promotes Ogt transcription through cooperation with the histone acetyltransferase p300 and transcription factor CCAAT/enhancer-binding protein β (C/EBPβ).
30037904	7	22	theme	primary	1386:1392	arg1	PDAC					1434:1437	PDAC	1434:1437	PDAC	1434:1437	Using a Kras -driven primary mouse pancreatic ductal adenocarcinoma (PDAC) cell line, we found that inhibition of extracellular signal-regulated kinase (ERK) signaling decreases OGA glycosidase activity and reduces OGT mRNA and protein levels, suggesting that ERK signaling may alter O-GlcNAc homeostasis in PDAC by modulating OGA-mediated Ogt transcription.
30037904	7	22	theme	primary	1386:1392	arg1	adenocarcinoma					1418:1431	a Kras -driven primary mouse pancreatic ductal adenocarcinoma	1371:1431	a Kras -driven primary mouse pancreatic ductal adenocarcinoma (PDAC) cell line	1371:1448	Using a Kras -driven primary mouse pancreatic ductal adenocarcinoma (PDAC) cell line, we found that inhibition of extracellular signal-regulated kinase (ERK) signaling decreases OGA glycosidase activity and reduces OGT mRNA and protein levels, suggesting that ERK signaling may alter O-GlcNAc homeostasis in PDAC by modulating OGA-mediated Ogt transcription.
30037904	0	23	theme	pancreatic	67:76	arg1	cancer					78:83	pancreatic cancer	67:83	pancreatic cancer	67:83	Transcriptional regulation of O-GlcNAc homeostasis is disrupted in pancreatic cancer.
30037904	5	24	theme	Ogt	937:939	arg1	transcription					941:953	Ogt transcription	937:953	Ogt transcription	937:953	Specifically, we found that OGA promotes Ogt transcription through cooperation with the histone acetyltransferase p300 and transcription factor CCAAT/enhancer-binding protein β (C/EBPβ).
30037904	7	25	theme	ERK	1625:1627	arg1	signaling					1629:1637	ERK signaling	1625:1637	ERK signaling	1625:1637	Using a Kras -driven primary mouse pancreatic ductal adenocarcinoma (PDAC) cell line, we found that inhibition of extracellular signal-regulated kinase (ERK) signaling decreases OGA glycosidase activity and reduces OGT mRNA and protein levels, suggesting that ERK signaling may alter O-GlcNAc homeostasis in PDAC by modulating OGA-mediated Ogt transcription.
30037904	4	26	theme	mechanism	747:755	arg1	existence					720:728	the existence	716:728	the existence of a homeostatic mechanism involving mutual regulation of the O-GlcNAc-cycling enzymes O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA) at the transcriptional level	716:893	Here, we demonstrate the existence of a homeostatic mechanism involving mutual regulation of the O-GlcNAc-cycling enzymes O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA) at the transcriptional level.
30037904	6	27	theme	OGT	1243:1245	arg1	levels					1266:1271	OGT and OGA expression levels	1243:1271	OGT and OGA expression levels	1243:1271	To examine the role of mutual regulation of OGT and OGA in the disease state, we analyzed gene expression data from human cancer data sets, which revealed that OGT and OGA expression levels are highly correlated in numerous human cancers, particularly in pancreatic adenocarcinoma.
30037904	6	28	from	role	1098:1101	arg1	state					1154:1158	the disease state	1142:1158	the disease state	1142:1158	To examine the role of mutual regulation of OGT and OGA in the disease state, we analyzed gene expression data from human cancer data sets, which revealed that OGT and OGA expression levels are highly correlated in numerous human cancers, particularly in pancreatic adenocarcinoma.
30037904	2	29	contain	have	399:402	arg1	excess					346:351	excess	346:351	excess	346:351	Accumulating evidence indicates that both excess and deficiency of protein O-GlcNAcylation can have deleterious effects on the cell, suggesting that maintenance of O-GlcNAc homeostasis is essential for proper cellular function.
30037904	2	29	contain	have	399:402	arg2	effects					416:422	deleterious effects	404:422	deleterious effects	404:422	Accumulating evidence indicates that both excess and deficiency of protein O-GlcNAcylation can have deleterious effects on the cell, suggesting that maintenance of O-GlcNAc homeostasis is essential for proper cellular function.
30037904	2	29	contain	have	399:402	arg1	deficiency					357:366	deficiency	357:366	deficiency	357:366	Accumulating evidence indicates that both excess and deficiency of protein O-GlcNAcylation can have deleterious effects on the cell, suggesting that maintenance of O-GlcNAc homeostasis is essential for proper cellular function.
30037904	5	30	theme	CCAAT/enhancer-binding	1040:1061	arg1	protein					1063:1069	CCAAT/enhancer-binding protein β	1040:1071	transcription factor CCAAT/enhancer-binding protein β (C/EBPβ)	1019:1080	Specifically, we found that OGA promotes Ogt transcription through cooperation with the histone acetyltransferase p300 and transcription factor CCAAT/enhancer-binding protein β (C/EBPβ).
30037904	5	30	theme	CCAAT/enhancer-binding	1040:1061	arg1	C/EBPβ					1074:1079	C/EBPβ	1074:1079	C/EBPβ	1074:1079	Specifically, we found that OGA promotes Ogt transcription through cooperation with the histone acetyltransferase p300 and transcription factor CCAAT/enhancer-binding protein β (C/EBPβ).
30037904	8	31	theme	human	1907:1911	arg1	disease					1913:1919	human disease	1907:1919	human disease	1907:1919	Our study elucidates a transcriptional mechanism that regulates cellular O-GlcNAc homeostasis, which may lay a foundation for exploring O-GlcNAc signaling as a therapeutic target for human disease.
30037904	1	32	theme	Many	86:89	arg1	proteins					105:112	Many intracellular proteins	86:112	Many intracellular proteins	86:112	Many intracellular proteins are reversibly modified by O-linked GlcNAc (O-GlcNAc), a post-translational modification that dynamically regulates fundamental cellular processes in response to diverse environmental cues.
30037904	7	33	theme	-driven	1378:1384	arg1	PDAC					1434:1437	PDAC	1434:1437	PDAC	1434:1437	Using a Kras -driven primary mouse pancreatic ductal adenocarcinoma (PDAC) cell line, we found that inhibition of extracellular signal-regulated kinase (ERK) signaling decreases OGA glycosidase activity and reduces OGT mRNA and protein levels, suggesting that ERK signaling may alter O-GlcNAc homeostasis in PDAC by modulating OGA-mediated Ogt transcription.
30037904	7	33	theme	-driven	1378:1384	arg1	adenocarcinoma					1418:1431	a Kras -driven primary mouse pancreatic ductal adenocarcinoma	1371:1431	a Kras -driven primary mouse pancreatic ductal adenocarcinoma (PDAC) cell line	1371:1448	Using a Kras -driven primary mouse pancreatic ductal adenocarcinoma (PDAC) cell line, we found that inhibition of extracellular signal-regulated kinase (ERK) signaling decreases OGA glycosidase activity and reduces OGT mRNA and protein levels, suggesting that ERK signaling may alter O-GlcNAc homeostasis in PDAC by modulating OGA-mediated Ogt transcription.
30037904	5	34	with	cooperation	963:973	arg1	p300					1010:1013	the histone acetyltransferase p300	980:1013	the histone acetyltransferase p300	980:1013	Specifically, we found that OGA promotes Ogt transcription through cooperation with the histone acetyltransferase p300 and transcription factor CCAAT/enhancer-binding protein β (C/EBPβ).
30037904	5	34	with	cooperation	963:973	arg1	factor					1033:1038	transcription factor	1019:1038	transcription factor CCAAT/enhancer-binding protein β (C/EBPβ)	1019:1080	Specifically, we found that OGA promotes Ogt transcription through cooperation with the histone acetyltransferase p300 and transcription factor CCAAT/enhancer-binding protein β (C/EBPβ).
30037904	4	35	theme	O-GlcNAc-cycling	792:807	arg1	enzymes					809:815	the O-GlcNAc-cycling enzymes	788:815	the O-GlcNAc-cycling enzymes O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA)	788:864	Here, we demonstrate the existence of a homeostatic mechanism involving mutual regulation of the O-GlcNAc-cycling enzymes O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA) at the transcriptional level.
30037904	4	35	theme	O-GlcNAc-cycling	792:807	arg1	O-GlcNAcase					848:858	O-GlcNAcase	848:858	O-GlcNAcase (OGA)	848:864	Here, we demonstrate the existence of a homeostatic mechanism involving mutual regulation of the O-GlcNAc-cycling enzymes O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA) at the transcriptional level.
30037904	4	35	theme	O-GlcNAc-cycling	792:807	arg1	transferase					826:836	O-GlcNAc transferase	817:836	O-GlcNAc transferase (OGT)	817:842	Here, we demonstrate the existence of a homeostatic mechanism involving mutual regulation of the O-GlcNAc-cycling enzymes O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA) at the transcriptional level.
30037904	0	36	theme	Transcriptional	0:14	arg1	regulation					16:25	Transcriptional regulation	0:25	Transcriptional regulation of O-GlcNAc homeostasis	0:49	Transcriptional regulation of O-GlcNAc homeostasis is disrupted in pancreatic cancer.
30037904	1	37	mod	modified	129:136	arg1	proteins					105:112	Many intracellular proteins	86:112	Many intracellular proteins	86:112	Many intracellular proteins are reversibly modified by O-linked GlcNAc (O-GlcNAc), a post-translational modification that dynamically regulates fundamental cellular processes in response to diverse environmental cues.
30037904	1	37	mod	modified	129:136	arg3	modification					190:201	a post-translational modification	169:201	a post-translational modification that dynamically regulates fundamental cellular processes in response to diverse environmental cues	169:301	Many intracellular proteins are reversibly modified by O-linked GlcNAc (O-GlcNAc), a post-translational modification that dynamically regulates fundamental cellular processes in response to diverse environmental cues.
30037904	1	37	mod	modified	129:136	arg3	GlcNAc					150:155	O-linked GlcNAc	141:155	O-linked GlcNAc (O-GlcNAc)	141:166	Many intracellular proteins are reversibly modified by O-linked GlcNAc (O-GlcNAc), a post-translational modification that dynamically regulates fundamental cellular processes in response to diverse environmental cues.
30037904	1	37	mod	modified	129:136	arg3	O-GlcNAc					158:165	O-GlcNAc	158:165	O-GlcNAc	158:165	Many intracellular proteins are reversibly modified by O-linked GlcNAc (O-GlcNAc), a post-translational modification that dynamically regulates fundamental cellular processes in response to diverse environmental cues.
30037904	4	38	theme	O-GlcNAc	817:824	arg1	enzymes					809:815	the O-GlcNAc-cycling enzymes	788:815	the O-GlcNAc-cycling enzymes O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA)	788:864	Here, we demonstrate the existence of a homeostatic mechanism involving mutual regulation of the O-GlcNAc-cycling enzymes O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA) at the transcriptional level.
30037904	4	38	theme	O-GlcNAc	817:824	arg1	OGT					839:841	OGT	839:841	OGT	839:841	Here, we demonstrate the existence of a homeostatic mechanism involving mutual regulation of the O-GlcNAc-cycling enzymes O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA) at the transcriptional level.
30037904	4	38	theme	O-GlcNAc	817:824	arg1	transferase					826:836	O-GlcNAc transferase	817:836	O-GlcNAc transferase (OGT)	817:842	Here, we demonstrate the existence of a homeostatic mechanism involving mutual regulation of the O-GlcNAc-cycling enzymes O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA) at the transcriptional level.
30037904	2	39	theme	O-GlcNAc	468:475	arg1	homeostasis					477:487	O-GlcNAc homeostasis	468:487	O-GlcNAc homeostasis	468:487	Accumulating evidence indicates that both excess and deficiency of protein O-GlcNAcylation can have deleterious effects on the cell, suggesting that maintenance of O-GlcNAc homeostasis is essential for proper cellular function.
30037904	7	40	theme	mRNA	1584:1587	arg1	levels					1601:1606	OGT mRNA and protein levels	1580:1606	OGT mRNA and protein levels	1580:1606	Using a Kras -driven primary mouse pancreatic ductal adenocarcinoma (PDAC) cell line, we found that inhibition of extracellular signal-regulated kinase (ERK) signaling decreases OGA glycosidase activity and reduces OGT mRNA and protein levels, suggesting that ERK signaling may alter O-GlcNAc homeostasis in PDAC by modulating OGA-mediated Ogt transcription.
30037904	5	41	theme	transcription	1019:1031	arg1	factor					1033:1038	transcription factor	1019:1038	transcription factor CCAAT/enhancer-binding protein β (C/EBPβ)	1019:1080	Specifically, we found that OGA promotes Ogt transcription through cooperation with the histone acetyltransferase p300 and transcription factor CCAAT/enhancer-binding protein β (C/EBPβ).
30037904	7	42	theme	Ogt	1705:1707	arg1	transcription					1709:1721	OGA-mediated Ogt transcription	1692:1721	OGA-mediated Ogt transcription	1692:1721	Using a Kras -driven primary mouse pancreatic ductal adenocarcinoma (PDAC) cell line, we found that inhibition of extracellular signal-regulated kinase (ERK) signaling decreases OGA glycosidase activity and reduces OGT mRNA and protein levels, suggesting that ERK signaling may alter O-GlcNAc homeostasis in PDAC by modulating OGA-mediated Ogt transcription.
30037904	6	43	theme	regulation	1113:1122	arg1	role					1098:1101	the role	1094:1101	the role of mutual regulation of OGT and OGA in the disease state	1094:1158	To examine the role of mutual regulation of OGT and OGA in the disease state, we analyzed gene expression data from human cancer data sets, which revealed that OGT and OGA expression levels are highly correlated in numerous human cancers, particularly in pancreatic adenocarcinoma.
30037904	0	44	theme	homeostasis	39:49	arg1	regulation					16:25	Transcriptional regulation	0:25	Transcriptional regulation of O-GlcNAc homeostasis	0:49	Transcriptional regulation of O-GlcNAc homeostasis is disrupted in pancreatic cancer.
30037904	2	45	theme	cellular	513:520	arg1	function					522:529	proper cellular function	506:529	proper cellular function	506:529	Accumulating evidence indicates that both excess and deficiency of protein O-GlcNAcylation can have deleterious effects on the cell, suggesting that maintenance of O-GlcNAc homeostasis is essential for proper cellular function.
30037904	1	46	link	O-linked	141:148	arg1	modification					190:201	a post-translational modification	169:201	a post-translational modification that dynamically regulates fundamental cellular processes in response to diverse environmental cues	169:301	Many intracellular proteins are reversibly modified by O-linked GlcNAc (O-GlcNAc), a post-translational modification that dynamically regulates fundamental cellular processes in response to diverse environmental cues.
30037904	1	46	link	O-linked	141:148	arg1	O-GlcNAc					158:165	O-GlcNAc	158:165	O-GlcNAc	158:165	Many intracellular proteins are reversibly modified by O-linked GlcNAc (O-GlcNAc), a post-translational modification that dynamically regulates fundamental cellular processes in response to diverse environmental cues.
30037904	1	46	link	O-linked	141:148	arg1	GlcNAc					150:155	O-linked GlcNAc	141:155	O-linked GlcNAc (O-GlcNAc)	141:166	Many intracellular proteins are reversibly modified by O-linked GlcNAc (O-GlcNAc), a post-translational modification that dynamically regulates fundamental cellular processes in response to diverse environmental cues.
30037904	4	47	theme	transcriptional	873:887	arg1	level					889:893	the transcriptional level	869:893	the transcriptional level	869:893	Here, we demonstrate the existence of a homeostatic mechanism involving mutual regulation of the O-GlcNAc-cycling enzymes O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA) at the transcriptional level.
30037904	6	48	theme	gene	1173:1176	arg1	data					1189:1192	gene expression data	1173:1192	gene expression data from human cancer data sets	1173:1220	To examine the role of mutual regulation of OGT and OGA in the disease state, we analyzed gene expression data from human cancer data sets, which revealed that OGT and OGA expression levels are highly correlated in numerous human cancers, particularly in pancreatic adenocarcinoma.
30037904	6	49	dep	analyzed	1164:1171	arg1	revealed					1229:1236	revealed	1229:1236	revealed that OGT and OGA expression levels are highly correlated in numerous human cancers, particularly in pancreatic adenocarcinoma	1229:1362	To examine the role of mutual regulation of OGT and OGA in the disease state, we analyzed gene expression data from human cancer data sets, which revealed that OGT and OGA expression levels are highly correlated in numerous human cancers, particularly in pancreatic adenocarcinoma.
30037904	2	50	theme	protein	371:377	arg1	O-GlcNAcylation					379:393	protein O-GlcNAcylation	371:393	protein O-GlcNAcylation	371:393	Accumulating evidence indicates that both excess and deficiency of protein O-GlcNAcylation can have deleterious effects on the cell, suggesting that maintenance of O-GlcNAc homeostasis is essential for proper cellular function.
30037904	6	51	from	cancers	1313:1319	arg1	adenocarcinoma					1349:1362	pancreatic adenocarcinoma	1338:1362	pancreatic adenocarcinoma	1338:1362	To examine the role of mutual regulation of OGT and OGA in the disease state, we analyzed gene expression data from human cancer data sets, which revealed that OGT and OGA expression levels are highly correlated in numerous human cancers, particularly in pancreatic adenocarcinoma.
30037904	7	52	theme	OGA	1543:1545	arg1	activity					1559:1566	OGA glycosidase activity	1543:1566	OGA glycosidase activity	1543:1566	Using a Kras -driven primary mouse pancreatic ductal adenocarcinoma (PDAC) cell line, we found that inhibition of extracellular signal-regulated kinase (ERK) signaling decreases OGA glycosidase activity and reduces OGT mRNA and protein levels, suggesting that ERK signaling may alter O-GlcNAc homeostasis in PDAC by modulating OGA-mediated Ogt transcription.
30037904	3	53	theme	O-GlcNAc	570:577	arg1	homeostasis					579:589	O-GlcNAc homeostasis	570:589	O-GlcNAc homeostasis	570:589	However, the mechanisms through which O-GlcNAc homeostasis is maintained in the physiologic state and altered in the disease state have not yet been investigated.
30037904	7	54	theme	signaling	1523:1531	arg1	inhibition					1465:1474	inhibition	1465:1474	inhibition of extracellular signal-regulated kinase (ERK) signaling	1465:1531	Using a Kras -driven primary mouse pancreatic ductal adenocarcinoma (PDAC) cell line, we found that inhibition of extracellular signal-regulated kinase (ERK) signaling decreases OGA glycosidase activity and reduces OGT mRNA and protein levels, suggesting that ERK signaling may alter O-GlcNAc homeostasis in PDAC by modulating OGA-mediated Ogt transcription.
30037904	2	55	theme	deleterious	404:414	arg1	effects					416:422	deleterious effects	404:422	deleterious effects	404:422	Accumulating evidence indicates that both excess and deficiency of protein O-GlcNAcylation can have deleterious effects on the cell, suggesting that maintenance of O-GlcNAc homeostasis is essential for proper cellular function.
30037904	1	56	theme	diverse	276:282	arg1	cues					298:301	diverse environmental cues	276:301	diverse environmental cues	276:301	Many intracellular proteins are reversibly modified by O-linked GlcNAc (O-GlcNAc), a post-translational modification that dynamically regulates fundamental cellular processes in response to diverse environmental cues.
30037904	7	57	theme	ERK	1518:1520	arg1	signaling					1523:1531	extracellular signal-regulated kinase (ERK) signaling	1479:1531	extracellular signal-regulated kinase (ERK) signaling	1479:1531	Using a Kras -driven primary mouse pancreatic ductal adenocarcinoma (PDAC) cell line, we found that inhibition of extracellular signal-regulated kinase (ERK) signaling decreases OGA glycosidase activity and reduces OGT mRNA and protein levels, suggesting that ERK signaling may alter O-GlcNAc homeostasis in PDAC by modulating OGA-mediated Ogt transcription.
30037904	7	58	theme	kinase	1510:1515	arg1	ERK					1518:1520	extracellular signal-regulated kinase (ERK)	1479:1521	extracellular signal-regulated kinase (ERK) signaling	1479:1531	Using a Kras -driven primary mouse pancreatic ductal adenocarcinoma (PDAC) cell line, we found that inhibition of extracellular signal-regulated kinase (ERK) signaling decreases OGA glycosidase activity and reduces OGT mRNA and protein levels, suggesting that ERK signaling may alter O-GlcNAc homeostasis in PDAC by modulating OGA-mediated Ogt transcription.
30037904	6	59	theme	numerous	1298:1305	arg1	cancers					1313:1319	numerous human cancers	1298:1319	numerous human cancers	1298:1319	To examine the role of mutual regulation of OGT and OGA in the disease state, we analyzed gene expression data from human cancer data sets, which revealed that OGT and OGA expression levels are highly correlated in numerous human cancers, particularly in pancreatic adenocarcinoma.
30037904	7	60	theme	extracellular	1479:1491	arg1	ERK					1518:1520	extracellular signal-regulated kinase (ERK)	1479:1521	extracellular signal-regulated kinase (ERK) signaling	1479:1531	Using a Kras -driven primary mouse pancreatic ductal adenocarcinoma (PDAC) cell line, we found that inhibition of extracellular signal-regulated kinase (ERK) signaling decreases OGA glycosidase activity and reduces OGT mRNA and protein levels, suggesting that ERK signaling may alter O-GlcNAc homeostasis in PDAC by modulating OGA-mediated Ogt transcription.
30037904	6	61	theme	data	1212:1215	arg1	sets					1217:1220	human cancer data sets	1199:1220	human cancer data sets	1199:1220	To examine the role of mutual regulation of OGT and OGA in the disease state, we analyzed gene expression data from human cancer data sets, which revealed that OGT and OGA expression levels are highly correlated in numerous human cancers, particularly in pancreatic adenocarcinoma.
30037904	6	62	theme	human	1199:1203	arg1	sets					1217:1220	human cancer data sets	1199:1220	human cancer data sets	1199:1220	To examine the role of mutual regulation of OGT and OGA in the disease state, we analyzed gene expression data from human cancer data sets, which revealed that OGT and OGA expression levels are highly correlated in numerous human cancers, particularly in pancreatic adenocarcinoma.
30037904	3	63	theme	physiologic	612:622	arg1	state					624:628	the physiologic state	608:628	the physiologic state	608:628	However, the mechanisms through which O-GlcNAc homeostasis is maintained in the physiologic state and altered in the disease state have not yet been investigated.
30037904	8	64	theme	O-GlcNAc	1797:1804	arg1	homeostasis					1806:1816	cellular O-GlcNAc homeostasis	1788:1816	cellular O-GlcNAc homeostasis	1788:1816	Our study elucidates a transcriptional mechanism that regulates cellular O-GlcNAc homeostasis, which may lay a foundation for exploring O-GlcNAc signaling as a therapeutic target for human disease.
30037904	3	65	theme	disease	649:655	arg1	state					657:661	the disease state	645:661	the disease state	645:661	However, the mechanisms through which O-GlcNAc homeostasis is maintained in the physiologic state and altered in the disease state have not yet been investigated.
30037904	1	66	theme	fundamental	230:240	arg1	processes					251:259	fundamental cellular processes	230:259	fundamental cellular processes	230:259	Many intracellular proteins are reversibly modified by O-linked GlcNAc (O-GlcNAc), a post-translational modification that dynamically regulates fundamental cellular processes in response to diverse environmental cues.
30037904	6	67	theme	OGA	1251:1253	arg1	levels					1266:1271	OGT and OGA expression levels	1243:1271	OGT and OGA expression levels	1243:1271	To examine the role of mutual regulation of OGT and OGA in the disease state, we analyzed gene expression data from human cancer data sets, which revealed that OGT and OGA expression levels are highly correlated in numerous human cancers, particularly in pancreatic adenocarcinoma.
30037904	6	68	theme	pancreatic	1338:1347	arg1	adenocarcinoma					1349:1362	pancreatic adenocarcinoma	1338:1362	pancreatic adenocarcinoma	1338:1362	To examine the role of mutual regulation of OGT and OGA in the disease state, we analyzed gene expression data from human cancer data sets, which revealed that OGT and OGA expression levels are highly correlated in numerous human cancers, particularly in pancreatic adenocarcinoma.
30037904	1	69	theme	O-linked	141:148	arg1	modification					190:201	a post-translational modification	169:201	a post-translational modification that dynamically regulates fundamental cellular processes in response to diverse environmental cues	169:301	Many intracellular proteins are reversibly modified by O-linked GlcNAc (O-GlcNAc), a post-translational modification that dynamically regulates fundamental cellular processes in response to diverse environmental cues.
30037904	1	69	theme	O-linked	141:148	arg1	O-GlcNAc					158:165	O-GlcNAc	158:165	O-GlcNAc	158:165	Many intracellular proteins are reversibly modified by O-linked GlcNAc (O-GlcNAc), a post-translational modification that dynamically regulates fundamental cellular processes in response to diverse environmental cues.
30037904	1	69	theme	O-linked	141:148	arg1	GlcNAc					150:155	O-linked GlcNAc	141:155	O-linked GlcNAc (O-GlcNAc)	141:166	Many intracellular proteins are reversibly modified by O-linked GlcNAc (O-GlcNAc), a post-translational modification that dynamically regulates fundamental cellular processes in response to diverse environmental cues.
30037904	7	70	theme	ductal	1411:1416	arg1	PDAC					1434:1437	PDAC	1434:1437	PDAC	1434:1437	Using a Kras -driven primary mouse pancreatic ductal adenocarcinoma (PDAC) cell line, we found that inhibition of extracellular signal-regulated kinase (ERK) signaling decreases OGA glycosidase activity and reduces OGT mRNA and protein levels, suggesting that ERK signaling may alter O-GlcNAc homeostasis in PDAC by modulating OGA-mediated Ogt transcription.
30037904	7	70	theme	ductal	1411:1416	arg1	adenocarcinoma					1418:1431	a Kras -driven primary mouse pancreatic ductal adenocarcinoma	1371:1431	a Kras -driven primary mouse pancreatic ductal adenocarcinoma (PDAC) cell line	1371:1448	Using a Kras -driven primary mouse pancreatic ductal adenocarcinoma (PDAC) cell line, we found that inhibition of extracellular signal-regulated kinase (ERK) signaling decreases OGA glycosidase activity and reduces OGT mRNA and protein levels, suggesting that ERK signaling may alter O-GlcNAc homeostasis in PDAC by modulating OGA-mediated Ogt transcription.
30037904	7	71	theme	mouse	1394:1398	arg1	PDAC					1434:1437	PDAC	1434:1437	PDAC	1434:1437	Using a Kras -driven primary mouse pancreatic ductal adenocarcinoma (PDAC) cell line, we found that inhibition of extracellular signal-regulated kinase (ERK) signaling decreases OGA glycosidase activity and reduces OGT mRNA and protein levels, suggesting that ERK signaling may alter O-GlcNAc homeostasis in PDAC by modulating OGA-mediated Ogt transcription.
30037904	7	71	theme	mouse	1394:1398	arg1	adenocarcinoma					1418:1431	a Kras -driven primary mouse pancreatic ductal adenocarcinoma	1371:1431	a Kras -driven primary mouse pancreatic ductal adenocarcinoma (PDAC) cell line	1371:1448	Using a Kras -driven primary mouse pancreatic ductal adenocarcinoma (PDAC) cell line, we found that inhibition of extracellular signal-regulated kinase (ERK) signaling decreases OGA glycosidase activity and reduces OGT mRNA and protein levels, suggesting that ERK signaling may alter O-GlcNAc homeostasis in PDAC by modulating OGA-mediated Ogt transcription.
30037904	8	72	theme	O-GlcNAc	1860:1867	arg1	target					1896:1901	a therapeutic target	1882:1901	a therapeutic target for human disease	1882:1919	Our study elucidates a transcriptional mechanism that regulates cellular O-GlcNAc homeostasis, which may lay a foundation for exploring O-GlcNAc signaling as a therapeutic target for human disease.
30037904	8	72	theme	O-GlcNAc	1860:1867	arg1	signaling					1869:1877	O-GlcNAc signaling	1860:1877	O-GlcNAc signaling	1860:1877	Our study elucidates a transcriptional mechanism that regulates cellular O-GlcNAc homeostasis, which may lay a foundation for exploring O-GlcNAc signaling as a therapeutic target for human disease.
30037904	4	73	theme	homeostatic	735:745	arg1	mechanism					747:755	a homeostatic mechanism	733:755	a homeostatic mechanism involving mutual regulation of the O-GlcNAc-cycling enzymes O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA) at the transcriptional level	733:893	Here, we demonstrate the existence of a homeostatic mechanism involving mutual regulation of the O-GlcNAc-cycling enzymes O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA) at the transcriptional level.
30037904	7	74	from	homeostasis	1658:1668	arg1	PDAC					1673:1676	PDAC	1673:1676	PDAC	1673:1676	Using a Kras -driven primary mouse pancreatic ductal adenocarcinoma (PDAC) cell line, we found that inhibition of extracellular signal-regulated kinase (ERK) signaling decreases OGA glycosidase activity and reduces OGT mRNA and protein levels, suggesting that ERK signaling may alter O-GlcNAc homeostasis in PDAC by modulating OGA-mediated Ogt transcription.
30037904	7	75	theme	protein	1593:1599	arg1	levels					1601:1606	OGT mRNA and protein levels	1580:1606	OGT mRNA and protein levels	1580:1606	Using a Kras -driven primary mouse pancreatic ductal adenocarcinoma (PDAC) cell line, we found that inhibition of extracellular signal-regulated kinase (ERK) signaling decreases OGA glycosidase activity and reduces OGT mRNA and protein levels, suggesting that ERK signaling may alter O-GlcNAc homeostasis in PDAC by modulating OGA-mediated Ogt transcription.
30037904	6	76	from	sets	1217:1220	arg1	data					1189:1192	gene expression data	1173:1192	gene expression data from human cancer data sets	1173:1220	To examine the role of mutual regulation of OGT and OGA in the disease state, we analyzed gene expression data from human cancer data sets, which revealed that OGT and OGA expression levels are highly correlated in numerous human cancers, particularly in pancreatic adenocarcinoma.
30037904	8	77	theme	therapeutic	1884:1894	arg1	target					1896:1901	a therapeutic target	1882:1901	a therapeutic target for human disease	1882:1919	Our study elucidates a transcriptional mechanism that regulates cellular O-GlcNAc homeostasis, which may lay a foundation for exploring O-GlcNAc signaling as a therapeutic target for human disease.
30037904	8	77	theme	therapeutic	1884:1894	arg1	signaling					1869:1877	O-GlcNAc signaling	1860:1877	O-GlcNAc signaling	1860:1877	Our study elucidates a transcriptional mechanism that regulates cellular O-GlcNAc homeostasis, which may lay a foundation for exploring O-GlcNAc signaling as a therapeutic target for human disease.
30037904	1	78	theme	intracellular	91:103	arg1	proteins					105:112	Many intracellular proteins	86:112	Many intracellular proteins	86:112	Many intracellular proteins are reversibly modified by O-linked GlcNAc (O-GlcNAc), a post-translational modification that dynamically regulates fundamental cellular processes in response to diverse environmental cues.
30037904	0	79	theme	O-GlcNAc	30:37	arg1	homeostasis					39:49	O-GlcNAc homeostasis	30:49	O-GlcNAc homeostasis	30:49	Transcriptional regulation of O-GlcNAc homeostasis is disrupted in pancreatic cancer.
30037904	2	80	theme	homeostasis	477:487	arg1	maintenance					453:463	maintenance	453:463	maintenance of O-GlcNAc homeostasis	453:487	Accumulating evidence indicates that both excess and deficiency of protein O-GlcNAcylation can have deleterious effects on the cell, suggesting that maintenance of O-GlcNAc homeostasis is essential for proper cellular function.
30037904	4	81	theme	enzymes	809:815	arg1	regulation					774:783	mutual regulation	767:783	mutual regulation of the O-GlcNAc-cycling enzymes O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA)	767:864	Here, we demonstrate the existence of a homeostatic mechanism involving mutual regulation of the O-GlcNAc-cycling enzymes O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA) at the transcriptional level.
30037904	6	82	theme	mutual	1106:1111	arg1	regulation					1113:1122	mutual regulation	1106:1122	mutual regulation of OGT and OGA	1106:1137	To examine the role of mutual regulation of OGT and OGA in the disease state, we analyzed gene expression data from human cancer data sets, which revealed that OGT and OGA expression levels are highly correlated in numerous human cancers, particularly in pancreatic adenocarcinoma.
30037904	7	83	theme	OGT	1580:1582	arg1	mRNA					1584:1587	OGT mRNA	1580:1587	OGT mRNA	1580:1587	Using a Kras -driven primary mouse pancreatic ductal adenocarcinoma (PDAC) cell line, we found that inhibition of extracellular signal-regulated kinase (ERK) signaling decreases OGA glycosidase activity and reduces OGT mRNA and protein levels, suggesting that ERK signaling may alter O-GlcNAc homeostasis in PDAC by modulating OGA-mediated Ogt transcription.
30037904	6	84	theme	expression	1178:1187	arg1	data					1189:1192	gene expression data	1173:1192	gene expression data from human cancer data sets	1173:1220	To examine the role of mutual regulation of OGT and OGA in the disease state, we analyzed gene expression data from human cancer data sets, which revealed that OGT and OGA expression levels are highly correlated in numerous human cancers, particularly in pancreatic adenocarcinoma.
30037904	2	85	theme	proper	506:511	arg1	function					522:529	proper cellular function	506:529	proper cellular function	506:529	Accumulating evidence indicates that both excess and deficiency of protein O-GlcNAcylation can have deleterious effects on the cell, suggesting that maintenance of O-GlcNAc homeostasis is essential for proper cellular function.
30037904	2	86	theme	O-GlcNAcylation	379:393	arg1	excess					346:351	excess	346:351	excess	346:351	Accumulating evidence indicates that both excess and deficiency of protein O-GlcNAcylation can have deleterious effects on the cell, suggesting that maintenance of O-GlcNAc homeostasis is essential for proper cellular function.
30037904	2	86	theme	O-GlcNAcylation	379:393	arg1	deficiency					357:366	deficiency	357:366	deficiency	357:366	Accumulating evidence indicates that both excess and deficiency of protein O-GlcNAcylation can have deleterious effects on the cell, suggesting that maintenance of O-GlcNAc homeostasis is essential for proper cellular function.
30037904	7	87	theme	glycosidase	1547:1557	arg1	activity					1559:1566	OGA glycosidase activity	1543:1566	OGA glycosidase activity	1543:1566	Using a Kras -driven primary mouse pancreatic ductal adenocarcinoma (PDAC) cell line, we found that inhibition of extracellular signal-regulated kinase (ERK) signaling decreases OGA glycosidase activity and reduces OGT mRNA and protein levels, suggesting that ERK signaling may alter O-GlcNAc homeostasis in PDAC by modulating OGA-mediated Ogt transcription.
30037904	6	88	theme	OGA	1135:1137	arg1	regulation					1113:1122	mutual regulation	1106:1122	mutual regulation of OGT and OGA	1106:1137	To examine the role of mutual regulation of OGT and OGA in the disease state, we analyzed gene expression data from human cancer data sets, which revealed that OGT and OGA expression levels are highly correlated in numerous human cancers, particularly in pancreatic adenocarcinoma.
30214471	3	0	theme	anti-tumor	361:370	arg1	activity					372:379	anti-tumor activity	361:379	anti-tumor activity	361:379	However, the composition and anti-tumor activity of D. officinale grown in the Huoshan area are largely unknown.
30214471	4	1	dep	METHODS	445:451	arg1	DOPA-1					471:476	DOPA-1	471:476	DOPA-1	471:476	METHODS A polysaccharide (DOPA-1) was isolated from D. officinale by hot water extraction and ethanol precipitation, followed by purification via DEAE-cellulose and Sephadex G-100 chromatography.
30214471	4	1	dep	METHODS	445:451	arg1	polysaccharide					455:468	A polysaccharide	453:468	METHODS A polysaccharide (DOPA-1)	445:477	METHODS A polysaccharide (DOPA-1) was isolated from D. officinale by hot water extraction and ethanol precipitation, followed by purification via DEAE-cellulose and Sephadex G-100 chromatography.
30214471	10	2	theme	HepG-2	1282:1287	arg1	cells					1289:1293	DOPA-1-treated HepG-2 cells	1267:1293	DOPA-1-treated HepG-2 cells	1267:1293	Furthermore, apoptosis was observed in DOPA-1-treated HepG-2 cells along with Bcl-2 downregulation and Bax upregulation at the protein level.
30214471	11	3	theme	altered	1452:1458	arg1	function					1474:1481	altered mitochondrial function	1452:1481	altered mitochondrial function	1452:1481	CONCLUSIONS Our findings suggest that DOPA-1 induces apoptosis in tumor cells via altered mitochondrial function, ROS production, and altered apoptosis-related protein expression.
30214471	9	4	contain	had	1129:1131	arg2	potential					1217:1225	decreased mitochondrial membrane potential	1184:1225	decreased mitochondrial membrane potential	1184:1225	DOPA-1-treated HepG-2 cells also had increased reactive oxygen species (ROS) levels and decreased mitochondrial membrane potential.
30214471	9	4	contain	had	1129:1131	arg2	levels					1173:1178	increased reactive oxygen species (ROS) levels	1133:1178	increased reactive oxygen species (ROS) levels	1133:1178	DOPA-1-treated HepG-2 cells also had increased reactive oxygen species (ROS) levels and decreased mitochondrial membrane potential.
30214471	9	4	contain	had	1129:1131	arg1	cells					1118:1122	DOPA-1-treated HepG-2 cells	1096:1122	DOPA-1-treated HepG-2 cells	1096:1122	DOPA-1-treated HepG-2 cells also had increased reactive oxygen species (ROS) levels and decreased mitochondrial membrane potential.
30214471	5	5	theme	magnetic	685:692	arg1	resonance					694:702	nuclear magnetic resonance	677:702	nuclear magnetic resonance	677:702	DOPA-1 was analyzed by infrared and nuclear magnetic resonance and then characterized by periodate oxidation and Smith degradation.
30214471	9	6	theme	HepG-2	1111:1116	arg1	cells					1118:1122	DOPA-1-treated HepG-2 cells	1096:1122	DOPA-1-treated HepG-2 cells	1096:1122	DOPA-1-treated HepG-2 cells also had increased reactive oxygen species (ROS) levels and decreased mitochondrial membrane potential.
30214471	2	7	contain	have	303:306	arg2	variety					310:316	a variety	308:316	a variety of functions	308:329	Accumulating studies report that polysaccharides isolated from Dendrobium officinale have a variety of functions.
30214471	2	7	contain	have	303:306	arg1	polysaccharides					251:265	polysaccharides	251:265	polysaccharides isolated from Dendrobium officinale	251:301	Accumulating studies report that polysaccharides isolated from Dendrobium officinale have a variety of functions.
30214471	2	7	contain	have	303:306	arg2	functions					321:329	functions	321:329	functions	321:329	Accumulating studies report that polysaccharides isolated from Dendrobium officinale have a variety of functions.
30214471	10	8	located	observed	1255:1262	arg1	cells					1289:1293	DOPA-1-treated HepG-2 cells	1267:1293	DOPA-1-treated HepG-2 cells	1267:1293	Furthermore, apoptosis was observed in DOPA-1-treated HepG-2 cells along with Bcl-2 downregulation and Bax upregulation at the protein level.
30214471	10	8	located	observed	1255:1262	arg1	level					1363:1367	the protein level	1351:1367	the protein level	1351:1367	Furthermore, apoptosis was observed in DOPA-1-treated HepG-2 cells along with Bcl-2 downregulation and Bax upregulation at the protein level.
30214471	10	8	located	observed	1255:1262	arg2	apoptosis					1241:1249	apoptosis	1241:1249	apoptosis	1241:1249	Furthermore, apoptosis was observed in DOPA-1-treated HepG-2 cells along with Bcl-2 downregulation and Bax upregulation at the protein level.
30214471	4	9	theme	ethanol	539:545	arg1	precipitation					547:559	ethanol precipitation	539:559	ethanol precipitation	539:559	METHODS A polysaccharide (DOPA-1) was isolated from D. officinale by hot water extraction and ethanol precipitation, followed by purification via DEAE-cellulose and Sephadex G-100 chromatography.
30214471	7	10	theme	2.29 × 105 Da	1003:1015	arg1	weight					993:998	an average molecular weight	972:998	an average molecular weight of 2.29 × 105 Da	972:1015	RESULTS Our results show that DOPA-1 is mainly comprised of mannose, glucose, and galactose at a molar ratio of 1:0.42:0.27 and has an average molecular weight of 2.29 × 105 Da.
30214471	4	11	dep	isolated	483:490	arg1	followed					562:569	followed	562:569	followed by purification via DEAE-cellulose and Sephadex G-100 chromatography	562:638	METHODS A polysaccharide (DOPA-1) was isolated from D. officinale by hot water extraction and ethanol precipitation, followed by purification via DEAE-cellulose and Sephadex G-100 chromatography.
30214471	8	12	theme	dose-dependent	1073:1086	arg1	manner					1088:1093	a dose-dependent manner	1071:1093	a dose-dependent manner	1071:1093	Additionally, DOPA-1 inhibited HepG-2 cell growth in a dose-dependent manner.
30214471	7	13	theme	molecular	983:991	arg1	weight					993:998	an average molecular weight	972:998	an average molecular weight of 2.29 × 105 Da	972:1015	RESULTS Our results show that DOPA-1 is mainly comprised of mannose, glucose, and galactose at a molar ratio of 1:0.42:0.27 and has an average molecular weight of 2.29 × 105 Da.
30214471	11	14	theme	mitochondrial	1460:1472	arg1	function					1474:1481	altered mitochondrial function	1452:1481	altered mitochondrial function	1452:1481	CONCLUSIONS Our findings suggest that DOPA-1 induces apoptosis in tumor cells via altered mitochondrial function, ROS production, and altered apoptosis-related protein expression.
30214471	6	15	theme	anti-tumor	777:786	arg1	activity					788:795	The anti-tumor activity	773:795	The anti-tumor activity of DOPA-1	773:805	The anti-tumor activity of DOPA-1 was then tested in HepG-2 cells.
30214471	2	16	attach	isolated	267:274	arg2	polysaccharides					251:265	polysaccharides	251:265	polysaccharides isolated from Dendrobium officinale	251:301	Accumulating studies report that polysaccharides isolated from Dendrobium officinale have a variety of functions.
30214471	2	16	attach	isolated	267:274	arg1	officinale					292:301	Dendrobium officinale	281:301	Dendrobium officinale	281:301	Accumulating studies report that polysaccharides isolated from Dendrobium officinale have a variety of functions.
30214471	3	17	dep	composition	345:355	arg1	the					341:343	the	341:343	the	341:343	However, the composition and anti-tumor activity of D. officinale grown in the Huoshan area are largely unknown.
30214471	11	18	theme	apoptosis-related	1512:1528	arg1	expression					1538:1547	altered apoptosis-related protein expression	1504:1547	altered apoptosis-related protein expression	1504:1547	CONCLUSIONS Our findings suggest that DOPA-1 induces apoptosis in tumor cells via altered mitochondrial function, ROS production, and altered apoptosis-related protein expression.
30214471	9	19	theme	DOPA-1-treated	1096:1109	arg1	cells					1118:1122	DOPA-1-treated HepG-2 cells	1096:1122	DOPA-1-treated HepG-2 cells	1096:1122	DOPA-1-treated HepG-2 cells also had increased reactive oxygen species (ROS) levels and decreased mitochondrial membrane potential.
30214471	2	20	theme	functions	321:329	arg1	functions					321:329	functions	321:329	functions	321:329	Accumulating studies report that polysaccharides isolated from Dendrobium officinale have a variety of functions.
30214471	2	20	theme	functions	321:329	arg1	variety					310:316	a variety	308:316	a variety of functions	308:329	Accumulating studies report that polysaccharides isolated from Dendrobium officinale have a variety of functions.
30214471	10	21	theme	protein	1355:1361	arg1	level					1363:1367	the protein level	1351:1367	the protein level	1351:1367	Furthermore, apoptosis was observed in DOPA-1-treated HepG-2 cells along with Bcl-2 downregulation and Bax upregulation at the protein level.
30214471	1	22	theme	linked	189:194	arg1	units					211:215	linked monosaccharide units	189:215	linked monosaccharide units	189:215	BACKGROUND Polysaccharides are carbohydrate chains composed of linked monosaccharide units.
30214471	11	23	theme	ROS	1484:1486	arg1	production					1488:1497	ROS production	1484:1497	ROS production	1484:1497	CONCLUSIONS Our findings suggest that DOPA-1 induces apoptosis in tumor cells via altered mitochondrial function, ROS production, and altered apoptosis-related protein expression.
30214471	4	24	theme	water	518:522	arg1	extraction					524:533	hot water extraction	514:533	hot water extraction	514:533	METHODS A polysaccharide (DOPA-1) was isolated from D. officinale by hot water extraction and ethanol precipitation, followed by purification via DEAE-cellulose and Sephadex G-100 chromatography.
30214471	5	25	theme	nuclear	677:683	arg1	resonance					694:702	nuclear magnetic resonance	677:702	nuclear magnetic resonance	677:702	DOPA-1 was analyzed by infrared and nuclear magnetic resonance and then characterized by periodate oxidation and Smith degradation.
30214471	8	26	theme	HepG-2	1049:1054	arg1	growth					1061:1066	HepG-2 cell growth	1049:1066	HepG-2 cell growth	1049:1066	Additionally, DOPA-1 inhibited HepG-2 cell growth in a dose-dependent manner.
30214471	9	27	theme	increased	1133:1141	arg1	levels					1173:1178	increased reactive oxygen species (ROS) levels	1133:1178	increased reactive oxygen species (ROS) levels	1133:1178	DOPA-1-treated HepG-2 cells also had increased reactive oxygen species (ROS) levels and decreased mitochondrial membrane potential.
30214471	1	28	theme	monosaccharide	196:209	arg1	units					211:215	linked monosaccharide units	189:215	linked monosaccharide units	189:215	BACKGROUND Polysaccharides are carbohydrate chains composed of linked monosaccharide units.
30214471	7	29	contain	has	968:970	arg2	weight					993:998	an average molecular weight	972:998	an average molecular weight of 2.29 × 105 Da	972:1015	RESULTS Our results show that DOPA-1 is mainly comprised of mannose, glucose, and galactose at a molar ratio of 1:0.42:0.27 and has an average molecular weight of 2.29 × 105 Da.
30214471	7	29	contain	has	968:970	arg1	DOPA-1					870:875	DOPA-1	870:875	DOPA-1	870:875	RESULTS Our results show that DOPA-1 is mainly comprised of mannose, glucose, and galactose at a molar ratio of 1:0.42:0.27 and has an average molecular weight of 2.29 × 105 Da.
30214471	4	30	theme	hot	514:516	arg1	extraction					524:533	hot water extraction	514:533	hot water extraction	514:533	METHODS A polysaccharide (DOPA-1) was isolated from D. officinale by hot water extraction and ethanol precipitation, followed by purification via DEAE-cellulose and Sephadex G-100 chromatography.
30214471	1	31	link	linked	189:194	arg1	units					211:215	linked monosaccharide units	189:215	linked monosaccharide units	189:215	BACKGROUND Polysaccharides are carbohydrate chains composed of linked monosaccharide units.
30214471	7	32	theme	average	975:981	arg1	weight					993:998	an average molecular weight	972:998	an average molecular weight of 2.29 × 105 Da	972:1015	RESULTS Our results show that DOPA-1 is mainly comprised of mannose, glucose, and galactose at a molar ratio of 1:0.42:0.27 and has an average molecular weight of 2.29 × 105 Da.
30214471	9	33	theme	reactive	1143:1150	arg1	species					1159:1165	reactive oxygen species	1143:1165	increased reactive oxygen species (ROS) levels	1133:1178	DOPA-1-treated HepG-2 cells also had increased reactive oxygen species (ROS) levels and decreased mitochondrial membrane potential.
30214471	9	33	theme	reactive	1143:1150	arg1	ROS					1168:1170	ROS	1168:1170	ROS	1168:1170	DOPA-1-treated HepG-2 cells also had increased reactive oxygen species (ROS) levels and decreased mitochondrial membrane potential.
30214471	12	34	theme	bioactive	1555:1563	arg1	drug					1657:1660	an anti-tumor adjuvant drug	1634:1660	an anti-tumor adjuvant drug	1634:1660	This bioactive polysaccharide could, therefore, potentially be further developed as an anti-tumor adjuvant drug.
30214471	12	34	theme	bioactive	1555:1563	arg1	polysaccharide					1565:1578	This bioactive polysaccharide	1550:1578	This bioactive polysaccharide	1550:1578	This bioactive polysaccharide could, therefore, potentially be further developed as an anti-tumor adjuvant drug.
30214471	4	35	theme	DEAE-cellulose	591:604	arg1	chromatography					625:638	DEAE-cellulose and Sephadex G-100 chromatography	591:638	chromatography	625:638	METHODS A polysaccharide (DOPA-1) was isolated from D. officinale by hot water extraction and ethanol precipitation, followed by purification via DEAE-cellulose and Sephadex G-100 chromatography.
30214471	11	36	theme	CONCLUSIONS	1370:1380	arg1	findings					1386:1393	CONCLUSIONS Our findings	1370:1393	CONCLUSIONS Our findings	1370:1393	CONCLUSIONS Our findings suggest that DOPA-1 induces apoptosis in tumor cells via altered mitochondrial function, ROS production, and altered apoptosis-related protein expression.
30214471	9	37	theme	oxygen	1152:1157	arg1	species					1159:1165	reactive oxygen species	1143:1165	increased reactive oxygen species (ROS) levels	1133:1178	DOPA-1-treated HepG-2 cells also had increased reactive oxygen species (ROS) levels and decreased mitochondrial membrane potential.
30214471	9	37	theme	oxygen	1152:1157	arg1	ROS					1168:1170	ROS	1168:1170	ROS	1168:1170	DOPA-1-treated HepG-2 cells also had increased reactive oxygen species (ROS) levels and decreased mitochondrial membrane potential.
30214471	3	38	theme	Huoshan	411:417	arg1	area					419:422	the Huoshan area	407:422	the Huoshan area	407:422	However, the composition and anti-tumor activity of D. officinale grown in the Huoshan area are largely unknown.
30214471	8	39	theme	cell	1056:1059	arg1	growth					1061:1066	HepG-2 cell growth	1049:1066	HepG-2 cell growth	1049:1066	Additionally, DOPA-1 inhibited HepG-2 cell growth in a dose-dependent manner.
30214471	9	40	theme	species	1159:1165	arg1	levels					1173:1178	increased reactive oxygen species (ROS) levels	1133:1178	increased reactive oxygen species (ROS) levels	1133:1178	DOPA-1-treated HepG-2 cells also had increased reactive oxygen species (ROS) levels and decreased mitochondrial membrane potential.
30214471	0	41	theme	anti-tumor	21:30	arg1	activity					32:39	anti-tumor activity	21:39	anti-tumor activity	21:39	Characterization and anti-tumor activity of a polysaccharide isolated from Dendrobium officinale grown in the Huoshan County.
30214471	4	42	theme	Sephadex	610:617	arg1	chromatography					625:638	DEAE-cellulose and Sephadex G-100 chromatography	591:638	chromatography	625:638	METHODS A polysaccharide (DOPA-1) was isolated from D. officinale by hot water extraction and ethanol precipitation, followed by purification via DEAE-cellulose and Sephadex G-100 chromatography.
30214471	3	43	theme	officinale	387:396	arg1	composition					345:355	composition	345:355	composition	345:355	However, the composition and anti-tumor activity of D. officinale grown in the Huoshan area are largely unknown.
30214471	3	43	theme	officinale	387:396	arg1	activity					372:379	anti-tumor activity	361:379	anti-tumor activity	361:379	However, the composition and anti-tumor activity of D. officinale grown in the Huoshan area are largely unknown.
30214471	11	44	theme	tumor	1436:1440	arg1	cells					1442:1446	tumor cells	1436:1446	tumor cells	1436:1446	CONCLUSIONS Our findings suggest that DOPA-1 induces apoptosis in tumor cells via altered mitochondrial function, ROS production, and altered apoptosis-related protein expression.
30214471	11	45	theme	protein	1530:1536	arg1	expression					1538:1547	altered apoptosis-related protein expression	1504:1547	altered apoptosis-related protein expression	1504:1547	CONCLUSIONS Our findings suggest that DOPA-1 induces apoptosis in tumor cells via altered mitochondrial function, ROS production, and altered apoptosis-related protein expression.
30214471	10	46	theme	DOPA-1-treated	1267:1280	arg1	cells					1289:1293	DOPA-1-treated HepG-2 cells	1267:1293	DOPA-1-treated HepG-2 cells	1267:1293	Furthermore, apoptosis was observed in DOPA-1-treated HepG-2 cells along with Bcl-2 downregulation and Bax upregulation at the protein level.
30214471	2	47	theme	Dendrobium	281:290	arg1	officinale					292:301	Dendrobium officinale	281:301	Dendrobium officinale	281:301	Accumulating studies report that polysaccharides isolated from Dendrobium officinale have a variety of functions.
30214471	4	48	theme	G-100	619:623	arg1	chromatography					625:638	DEAE-cellulose and Sephadex G-100 chromatography	591:638	chromatography	625:638	METHODS A polysaccharide (DOPA-1) was isolated from D. officinale by hot water extraction and ethanol precipitation, followed by purification via DEAE-cellulose and Sephadex G-100 chromatography.
30214471	4	49	theme	D.	497:498	arg1	officinale					500:509	D. officinale	497:509	D. officinale	497:509	METHODS A polysaccharide (DOPA-1) was isolated from D. officinale by hot water extraction and ethanol precipitation, followed by purification via DEAE-cellulose and Sephadex G-100 chromatography.
30214471	6	50	theme	HepG-2	826:831	arg1	cells					833:837	HepG-2 cells	826:837	HepG-2 cells	826:837	The anti-tumor activity of DOPA-1 was then tested in HepG-2 cells.
30214471	9	51	theme	membrane	1208:1215	arg1	potential					1217:1225	decreased mitochondrial membrane potential	1184:1225	decreased mitochondrial membrane potential	1184:1225	DOPA-1-treated HepG-2 cells also had increased reactive oxygen species (ROS) levels and decreased mitochondrial membrane potential.
30214471	5	52	theme	periodate	730:738	arg1	oxidation					740:748	periodate oxidation	730:748	periodate oxidation	730:748	DOPA-1 was analyzed by infrared and nuclear magnetic resonance and then characterized by periodate oxidation and Smith degradation.
30214471	7	53	dep	RESULTS	840:846	arg1	show					860:863	show	860:863	show that DOPA-1 is mainly comprised of mannose, glucose, and galactose at a molar ratio of 1:0.42:0.27 and has an average molecular weight of 2.29 × 105 Da	860:1015	RESULTS Our results show that DOPA-1 is mainly comprised of mannose, glucose, and galactose at a molar ratio of 1:0.42:0.27 and has an average molecular weight of 2.29 × 105 Da.
30214471	12	54	theme	anti-tumor	1637:1646	arg1	drug					1657:1660	an anti-tumor adjuvant drug	1634:1660	an anti-tumor adjuvant drug	1634:1660	This bioactive polysaccharide could, therefore, potentially be further developed as an anti-tumor adjuvant drug.
30214471	12	54	theme	anti-tumor	1637:1646	arg1	polysaccharide					1565:1578	This bioactive polysaccharide	1550:1578	This bioactive polysaccharide	1550:1578	This bioactive polysaccharide could, therefore, potentially be further developed as an anti-tumor adjuvant drug.
30214471	0	55	theme	polysaccharide	46:59	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization and anti-tumor activity of a polysaccharide isolated from Dendrobium officinale grown in the Huoshan County.
30214471	0	55	theme	polysaccharide	46:59	arg1	activity					32:39	anti-tumor activity	21:39	anti-tumor activity	21:39	Characterization and anti-tumor activity of a polysaccharide isolated from Dendrobium officinale grown in the Huoshan County.
30214471	11	56	theme	altered	1504:1510	arg1	expression					1538:1547	altered apoptosis-related protein expression	1504:1547	altered apoptosis-related protein expression	1504:1547	CONCLUSIONS Our findings suggest that DOPA-1 induces apoptosis in tumor cells via altered mitochondrial function, ROS production, and altered apoptosis-related protein expression.
30214471	1	57	theme	BACKGROUND	126:135	arg1	Polysaccharides					137:151	BACKGROUND Polysaccharides	126:151	BACKGROUND Polysaccharides	126:151	BACKGROUND Polysaccharides are carbohydrate chains composed of linked monosaccharide units.
30214471	1	57	theme	BACKGROUND	126:135	arg1	chains					170:175	carbohydrate chains	157:175	carbohydrate chains composed of linked monosaccharide units	157:215	BACKGROUND Polysaccharides are carbohydrate chains composed of linked monosaccharide units.
30214471	10	58	theme	Bcl-2	1306:1310	arg1	downregulation					1312:1325	Bcl-2 downregulation	1306:1325	Bcl-2 downregulation	1306:1325	Furthermore, apoptosis was observed in DOPA-1-treated HepG-2 cells along with Bcl-2 downregulation and Bax upregulation at the protein level.
30214471	9	59	theme	decreased	1184:1192	arg1	potential					1217:1225	decreased mitochondrial membrane potential	1184:1225	decreased mitochondrial membrane potential	1184:1225	DOPA-1-treated HepG-2 cells also had increased reactive oxygen species (ROS) levels and decreased mitochondrial membrane potential.
30214471	7	60	theme	1:0.42:0.27	952:962	arg1	ratio					943:947	a molar ratio	935:947	a molar ratio of 1:0.42:0.27	935:962	RESULTS Our results show that DOPA-1 is mainly comprised of mannose, glucose, and galactose at a molar ratio of 1:0.42:0.27 and has an average molecular weight of 2.29 × 105 Da.
30214471	5	61	theme	Smith	754:758	arg1	degradation					760:770	Smith degradation	754:770	Smith degradation	754:770	DOPA-1 was analyzed by infrared and nuclear magnetic resonance and then characterized by periodate oxidation and Smith degradation.
30214471	9	62	theme	mitochondrial	1194:1206	arg1	potential					1217:1225	decreased mitochondrial membrane potential	1184:1225	decreased mitochondrial membrane potential	1184:1225	DOPA-1-treated HepG-2 cells also had increased reactive oxygen species (ROS) levels and decreased mitochondrial membrane potential.
30214471	7	63	theme	molar	937:941	arg1	ratio					943:947	a molar ratio	935:947	a molar ratio of 1:0.42:0.27	935:962	RESULTS Our results show that DOPA-1 is mainly comprised of mannose, glucose, and galactose at a molar ratio of 1:0.42:0.27 and has an average molecular weight of 2.29 × 105 Da.
30214471	12	64	theme	adjuvant	1648:1655	arg1	drug					1657:1660	an anti-tumor adjuvant drug	1634:1660	an anti-tumor adjuvant drug	1634:1660	This bioactive polysaccharide could, therefore, potentially be further developed as an anti-tumor adjuvant drug.
30214471	12	64	theme	adjuvant	1648:1655	arg1	polysaccharide					1565:1578	This bioactive polysaccharide	1550:1578	This bioactive polysaccharide	1550:1578	This bioactive polysaccharide could, therefore, potentially be further developed as an anti-tumor adjuvant drug.
30214471	0	65	theme	Dendrobium	75:84	arg1	officinale					86:95	Dendrobium officinale	75:95	Dendrobium officinale grown in the Huoshan County	75:123	Characterization and anti-tumor activity of a polysaccharide isolated from Dendrobium officinale grown in the Huoshan County.
30214471	2	66	theme	Accumulating	218:229	arg1	studies					231:237	Accumulating studies	218:237	Accumulating studies	218:237	Accumulating studies report that polysaccharides isolated from Dendrobium officinale have a variety of functions.
30214471	6	67	theme	DOPA-1	800:805	arg1	activity					788:795	The anti-tumor activity	773:795	The anti-tumor activity of DOPA-1	773:805	The anti-tumor activity of DOPA-1 was then tested in HepG-2 cells.
30214471	1	68	theme	carbohydrate	157:168	arg1	Polysaccharides					137:151	BACKGROUND Polysaccharides	126:151	BACKGROUND Polysaccharides	126:151	BACKGROUND Polysaccharides are carbohydrate chains composed of linked monosaccharide units.
30214471	1	68	theme	carbohydrate	157:168	arg1	chains					170:175	carbohydrate chains	157:175	carbohydrate chains composed of linked monosaccharide units	157:215	BACKGROUND Polysaccharides are carbohydrate chains composed of linked monosaccharide units.
30214471	4	69	attach	isolated	483:490	arg1	officinale					500:509	D. officinale	497:509	D. officinale	497:509	METHODS A polysaccharide (DOPA-1) was isolated from D. officinale by hot water extraction and ethanol precipitation, followed by purification via DEAE-cellulose and Sephadex G-100 chromatography.
30214471	4	69	attach	isolated	483:490	arg2	METHODS					445:451	METHODS A polysaccharide (DOPA-1)	445:477	METHODS A polysaccharide (DOPA-1)	445:477	METHODS A polysaccharide (DOPA-1) was isolated from D. officinale by hot water extraction and ethanol precipitation, followed by purification via DEAE-cellulose and Sephadex G-100 chromatography.
30214471	3	70	theme	D.	384:385	arg1	officinale					387:396	D. officinale	384:396	D. officinale grown in the Huoshan area	384:422	However, the composition and anti-tumor activity of D. officinale grown in the Huoshan area are largely unknown.
30214471	11	71	from	apoptosis	1423:1431	arg1	cells					1442:1446	tumor cells	1436:1446	tumor cells	1436:1446	CONCLUSIONS Our findings suggest that DOPA-1 induces apoptosis in tumor cells via altered mitochondrial function, ROS production, and altered apoptosis-related protein expression.
30214471	10	72	theme	Bax	1331:1333	arg1	upregulation					1335:1346	Bax upregulation	1331:1346	Bax upregulation	1331:1346	Furthermore, apoptosis was observed in DOPA-1-treated HepG-2 cells along with Bcl-2 downregulation and Bax upregulation at the protein level.
30296532	6	0	from	PFC	999:1001	arg1	levels					968:973	increased levels	958:973	increased levels of all NCAM isoforms in PFC upon LPS which was followed with a decrease in GluN1 and GluN2B subunits	958:1074	Furthermore, the absence of GluN2 A resulted in increased levels of all NCAM isoforms in PFC upon LPS which was followed with a decrease in GluN1 and GluN2B subunits.
30296532	3	1	theme	GluN2 A	490:496	arg1	subunit					498:504	GluN2 A subunit	490:504	GluN2 A subunit	490:504	Our data suggest that LPS-treated mice, lacking GluN2 A subunit, did not exhibit depressive-like behaviour.
30296532	7	2	located	present	1224:1230	arg1	PFC					1239:1241	the PFC	1235:1241	the PFC of KO mice	1235:1252	The levels of AMPA receptor subunits (GluA1, GluA3, and GluA4) in the hippocampus of GluN2 A mice were unaltered upon the treatment and abundantly present in the PFC of KO mice.
30296532	7	2	located	present	1224:1230	arg2	present					1224:1230	present	1224:1230	present	1224:1230	The levels of AMPA receptor subunits (GluA1, GluA3, and GluA4) in the hippocampus of GluN2 A mice were unaltered upon the treatment and abundantly present in the PFC of KO mice.
30296532	7	2	located	present	1224:1230	arg2	levels					1081:1086	The levels	1077:1086	The levels of AMPA receptor subunits (GluA1, GluA3, and GluA4) in the hippocampus of GluN2 A mice	1077:1173	The levels of AMPA receptor subunits (GluA1, GluA3, and GluA4) in the hippocampus of GluN2 A mice were unaltered upon the treatment and abundantly present in the PFC of KO mice.
30296532	7	2	located	present	1224:1230	arg2	unaltered					1180:1188	unaltered	1180:1188	unaltered	1180:1188	The levels of AMPA receptor subunits (GluA1, GluA3, and GluA4) in the hippocampus of GluN2 A mice were unaltered upon the treatment and abundantly present in the PFC of KO mice.
30296532	4	3	theme	unaltered	574:582	arg1	levels					584:589	unaltered levels	574:589	unaltered levels of IL-6	574:597	This was accompanied by unaltered levels of IL-6 and significant changes in neuroplasticity markers and glutamate receptor subunits composition in PFC and hippocampus.
30296532	7	4	theme	mice	1170:1173	arg1	hippocampus					1147:1157	the hippocampus	1143:1157	the hippocampus of GluN2 A mice	1143:1173	The levels of AMPA receptor subunits (GluA1, GluA3, and GluA4) in the hippocampus of GluN2 A mice were unaltered upon the treatment and abundantly present in the PFC of KO mice.
30296532	7	5	theme	AMPA	1091:1094	arg1	GluA3					1122:1126	GluA3	1122:1126	GluA3	1122:1126	The levels of AMPA receptor subunits (GluA1, GluA3, and GluA4) in the hippocampus of GluN2 A mice were unaltered upon the treatment and abundantly present in the PFC of KO mice.
30296532	7	5	theme	AMPA	1091:1094	arg1	GluA4					1133:1137	GluA4	1133:1137	GluA4	1133:1137	The levels of AMPA receptor subunits (GluA1, GluA3, and GluA4) in the hippocampus of GluN2 A mice were unaltered upon the treatment and abundantly present in the PFC of KO mice.
30296532	7	5	theme	AMPA	1091:1094	arg1	GluA1					1115:1119	GluA1	1115:1119	GluA1	1115:1119	The levels of AMPA receptor subunits (GluA1, GluA3, and GluA4) in the hippocampus of GluN2 A mice were unaltered upon the treatment and abundantly present in the PFC of KO mice.
30296532	7	5	theme	AMPA	1091:1094	arg1	subunits					1105:1112	AMPA receptor subunits	1091:1112	AMPA receptor subunits (GluA1, GluA3, and GluA4)	1091:1138	The levels of AMPA receptor subunits (GluA1, GluA3, and GluA4) in the hippocampus of GluN2 A mice were unaltered upon the treatment and abundantly present in the PFC of KO mice.
30296532	5	6	from	levels	887:892	arg1	hippocampus					897:907	hippocampus	897:907	hippocampus	897:907	In particular, an immune challenge in GluN2 A KO mice resulted in unchanged PSA-NCAM levels and proBDNF increase in both brain structures as well as in increase in BDNF levels in hippocampus.
30296532	8	7	from	signalling	1461:1470	arg1	PFC					1479:1481	PFC	1479:1481	PFC	1479:1481	These results indicate that the GluN2 A subunit is critical in neuroinflammation-related depression, that its absence abolishes LPS-induced depressive phenotype, sustains PSA-NCAM levels, increases proBDNF signalling in the PFC and hippocampus and potentiates synaptic stabilization through NCAM in the PFC upon an immune challenge.
30296532	8	7	from	signalling	1461:1470	arg1	hippocampus					1487:1497	hippocampus	1487:1497	hippocampus	1487:1497	These results indicate that the GluN2 A subunit is critical in neuroinflammation-related depression, that its absence abolishes LPS-induced depressive phenotype, sustains PSA-NCAM levels, increases proBDNF signalling in the PFC and hippocampus and potentiates synaptic stabilization through NCAM in the PFC upon an immune challenge.
30296532	6	8	theme	NCAM	982:985	arg1	isoforms					987:994	all NCAM isoforms	978:994	all NCAM isoforms in PFC upon LPS	978:1010	Furthermore, the absence of GluN2 A resulted in increased levels of all NCAM isoforms in PFC upon LPS which was followed with a decrease in GluN1 and GluN2B subunits.
30296532	8	9	dep	PFC	1479:1481	arg1	the					1475:1477	the	1475:1477	the	1475:1477	These results indicate that the GluN2 A subunit is critical in neuroinflammation-related depression, that its absence abolishes LPS-induced depressive phenotype, sustains PSA-NCAM levels, increases proBDNF signalling in the PFC and hippocampus and potentiates synaptic stabilization through NCAM in the PFC upon an immune challenge.
30296532	2	10	theme	receptor	293:300	arg1	subunit					277:283	GluN2 A subunit	269:283	GluN2 A subunit of NMDA receptor	269:300	In this study, we further investigated the role of GluN2 A subunit of NMDA receptor in synaptic processes in the prefrontal cortex (PFC) and hippocampus of GluN2 A knockout (KO) mice in LPS-induced depressive-like behavior.
30296532	2	11	from	processes	314:322	arg1	cortex					342:347	the prefrontal cortex	327:347	the prefrontal cortex (PFC)	327:353	In this study, we further investigated the role of GluN2 A subunit of NMDA receptor in synaptic processes in the prefrontal cortex (PFC) and hippocampus of GluN2 A knockout (KO) mice in LPS-induced depressive-like behavior.
30296532	2	11	from	processes	314:322	arg1	PFC					350:352	PFC	350:352	PFC	350:352	In this study, we further investigated the role of GluN2 A subunit of NMDA receptor in synaptic processes in the prefrontal cortex (PFC) and hippocampus of GluN2 A knockout (KO) mice in LPS-induced depressive-like behavior.
30296532	2	11	from	processes	314:322	arg1	hippocampus					359:369	hippocampus	359:369	hippocampus of GluN2 A knockout (KO) mice in LPS-induced depressive-like behavior	359:439	In this study, we further investigated the role of GluN2 A subunit of NMDA receptor in synaptic processes in the prefrontal cortex (PFC) and hippocampus of GluN2 A knockout (KO) mice in LPS-induced depressive-like behavior.
30296532	4	12	from	levels	584:589	arg1	markers					642:648	neuroplasticity markers	626:648	neuroplasticity markers	626:648	This was accompanied by unaltered levels of IL-6 and significant changes in neuroplasticity markers and glutamate receptor subunits composition in PFC and hippocampus.
30296532	4	12	from	levels	584:589	arg1	hippocampus					705:715	hippocampus	705:715	hippocampus	705:715	This was accompanied by unaltered levels of IL-6 and significant changes in neuroplasticity markers and glutamate receptor subunits composition in PFC and hippocampus.
30296532	4	12	from	levels	584:589	arg1	PFC					697:699	PFC	697:699	PFC	697:699	This was accompanied by unaltered levels of IL-6 and significant changes in neuroplasticity markers and glutamate receptor subunits composition in PFC and hippocampus.
30296532	7	13	attach	present	1224:1230	arg1	PFC					1239:1241	the PFC	1235:1241	the PFC of KO mice	1235:1252	The levels of AMPA receptor subunits (GluA1, GluA3, and GluA4) in the hippocampus of GluN2 A mice were unaltered upon the treatment and abundantly present in the PFC of KO mice.
30296532	7	13	attach	present	1224:1230	arg2	present					1224:1230	present	1224:1230	present	1224:1230	The levels of AMPA receptor subunits (GluA1, GluA3, and GluA4) in the hippocampus of GluN2 A mice were unaltered upon the treatment and abundantly present in the PFC of KO mice.
30296532	7	13	attach	present	1224:1230	arg2	levels					1081:1086	The levels	1077:1086	The levels of AMPA receptor subunits (GluA1, GluA3, and GluA4) in the hippocampus of GluN2 A mice	1077:1173	The levels of AMPA receptor subunits (GluA1, GluA3, and GluA4) in the hippocampus of GluN2 A mice were unaltered upon the treatment and abundantly present in the PFC of KO mice.
30296532	7	13	attach	present	1224:1230	arg2	unaltered					1180:1188	unaltered	1180:1188	unaltered	1180:1188	The levels of AMPA receptor subunits (GluA1, GluA3, and GluA4) in the hippocampus of GluN2 A mice were unaltered upon the treatment and abundantly present in the PFC of KO mice.
30296532	2	14	theme	NMDA	288:291	arg1	receptor					293:300	NMDA receptor	288:300	NMDA receptor	288:300	In this study, we further investigated the role of GluN2 A subunit of NMDA receptor in synaptic processes in the prefrontal cortex (PFC) and hippocampus of GluN2 A knockout (KO) mice in LPS-induced depressive-like behavior.
30296532	5	15	theme	brain	839:843	arg1	structures					845:854	both brain structures	834:854	both brain structures	834:854	In particular, an immune challenge in GluN2 A KO mice resulted in unchanged PSA-NCAM levels and proBDNF increase in both brain structures as well as in increase in BDNF levels in hippocampus.
30296532	2	16	from	cortex	342:347	arg1	behavior					432:439	LPS-induced depressive-like behavior	404:439	LPS-induced depressive-like behavior	404:439	In this study, we further investigated the role of GluN2 A subunit of NMDA receptor in synaptic processes in the prefrontal cortex (PFC) and hippocampus of GluN2 A knockout (KO) mice in LPS-induced depressive-like behavior.
30296532	1	17	theme	NMDA	203:206	arg1	receptor					208:215	NMDA receptor	203:215	NMDA receptor	203:215	Recent reports have demonstrated that lipopolysaccharide (LPS)-induced depressive-like behaviour is mediated via NMDA receptor.
30296532	4	18	theme	receptor	664:671	arg1	subunits					673:680	glutamate receptor subunits	654:680	glutamate receptor subunits	654:680	This was accompanied by unaltered levels of IL-6 and significant changes in neuroplasticity markers and glutamate receptor subunits composition in PFC and hippocampus.
30296532	7	19	theme	subunits	1105:1112	arg1	levels					1081:1086	The levels	1077:1086	The levels of AMPA receptor subunits (GluA1, GluA3, and GluA4) in the hippocampus of GluN2 A mice	1077:1173	The levels of AMPA receptor subunits (GluA1, GluA3, and GluA4) in the hippocampus of GluN2 A mice were unaltered upon the treatment and abundantly present in the PFC of KO mice.
30296532	7	19	theme	subunits	1105:1112	arg1	unaltered					1180:1188	unaltered	1180:1188	unaltered	1180:1188	The levels of AMPA receptor subunits (GluA1, GluA3, and GluA4) in the hippocampus of GluN2 A mice were unaltered upon the treatment and abundantly present in the PFC of KO mice.
30296532	7	19	theme	subunits	1105:1112	arg1	present					1224:1230	present	1224:1230	present	1224:1230	The levels of AMPA receptor subunits (GluA1, GluA3, and GluA4) in the hippocampus of GluN2 A mice were unaltered upon the treatment and abundantly present in the PFC of KO mice.
30296532	6	20	theme	isoforms	987:994	arg1	levels					968:973	increased levels	958:973	increased levels of all NCAM isoforms in PFC upon LPS which was followed with a decrease in GluN1 and GluN2B subunits	958:1074	Furthermore, the absence of GluN2 A resulted in increased levels of all NCAM isoforms in PFC upon LPS which was followed with a decrease in GluN1 and GluN2B subunits.
30296532	1	21	theme	Recent	90:95	arg1	reports					97:103	Recent reports	90:103	Recent reports	90:103	Recent reports have demonstrated that lipopolysaccharide (LPS)-induced depressive-like behaviour is mediated via NMDA receptor.
30296532	2	22	theme	knockout	382:389	arg1	mice					396:399	GluN2 A knockout (KO) mice	374:399	GluN2 A knockout (KO) mice	374:399	In this study, we further investigated the role of GluN2 A subunit of NMDA receptor in synaptic processes in the prefrontal cortex (PFC) and hippocampus of GluN2 A knockout (KO) mice in LPS-induced depressive-like behavior.
30296532	8	23	theme	synaptic	1515:1522	arg1	stabilization					1524:1536	synaptic stabilization	1515:1536	synaptic stabilization	1515:1536	These results indicate that the GluN2 A subunit is critical in neuroinflammation-related depression, that its absence abolishes LPS-induced depressive phenotype, sustains PSA-NCAM levels, increases proBDNF signalling in the PFC and hippocampus and potentiates synaptic stabilization through NCAM in the PFC upon an immune challenge.
30296532	6	24	from	isoforms	987:994	arg1	PFC					999:1001	PFC	999:1001	PFC upon LPS	999:1010	Furthermore, the absence of GluN2 A resulted in increased levels of all NCAM isoforms in PFC upon LPS which was followed with a decrease in GluN1 and GluN2B subunits.
30296532	5	25	theme	GluN2 A	756:762	arg1	mice					767:770	GluN2 A KO mice	756:770	GluN2 A KO mice	756:770	In particular, an immune challenge in GluN2 A KO mice resulted in unchanged PSA-NCAM levels and proBDNF increase in both brain structures as well as in increase in BDNF levels in hippocampus.
30296532	2	26	theme	GluN2 A	374:380	arg1	mice					396:399	GluN2 A knockout (KO) mice	374:399	GluN2 A knockout (KO) mice	374:399	In this study, we further investigated the role of GluN2 A subunit of NMDA receptor in synaptic processes in the prefrontal cortex (PFC) and hippocampus of GluN2 A knockout (KO) mice in LPS-induced depressive-like behavior.
30296532	5	27	theme	proBDNF	814:820	arg1	increase					822:829	proBDNF increase	814:829	proBDNF increase	814:829	In particular, an immune challenge in GluN2 A KO mice resulted in unchanged PSA-NCAM levels and proBDNF increase in both brain structures as well as in increase in BDNF levels in hippocampus.
30296532	2	28	from	hippocampus	359:369	arg1	behavior					432:439	LPS-induced depressive-like behavior	404:439	LPS-induced depressive-like behavior	404:439	In this study, we further investigated the role of GluN2 A subunit of NMDA receptor in synaptic processes in the prefrontal cortex (PFC) and hippocampus of GluN2 A knockout (KO) mice in LPS-induced depressive-like behavior.
30296532	7	29	dep	unaltered	1180:1188	arg1	levels					1081:1086	The levels	1077:1086	The levels of AMPA receptor subunits (GluA1, GluA3, and GluA4) in the hippocampus of GluN2 A mice	1077:1173	The levels of AMPA receptor subunits (GluA1, GluA3, and GluA4) in the hippocampus of GluN2 A mice were unaltered upon the treatment and abundantly present in the PFC of KO mice.
30296532	7	29	dep	unaltered	1180:1188	arg1	unaltered					1180:1188	unaltered	1180:1188	unaltered	1180:1188	The levels of AMPA receptor subunits (GluA1, GluA3, and GluA4) in the hippocampus of GluN2 A mice were unaltered upon the treatment and abundantly present in the PFC of KO mice.
30296532	7	29	dep	unaltered	1180:1188	arg1	present					1224:1230	present	1224:1230	present	1224:1230	The levels of AMPA receptor subunits (GluA1, GluA3, and GluA4) in the hippocampus of GluN2 A mice were unaltered upon the treatment and abundantly present in the PFC of KO mice.
30296532	2	30	from	role	261:264	arg1	processes					314:322	synaptic processes	305:322	synaptic processes in the prefrontal cortex (PFC) and hippocampus of GluN2 A knockout (KO) mice in LPS-induced depressive-like behavior	305:439	In this study, we further investigated the role of GluN2 A subunit of NMDA receptor in synaptic processes in the prefrontal cortex (PFC) and hippocampus of GluN2 A knockout (KO) mice in LPS-induced depressive-like behavior.
30296532	7	31	theme	mice	1249:1252	arg1	PFC					1239:1241	the PFC	1235:1241	the PFC of KO mice	1235:1252	The levels of AMPA receptor subunits (GluA1, GluA3, and GluA4) in the hippocampus of GluN2 A mice were unaltered upon the treatment and abundantly present in the PFC of KO mice.
30296532	2	32	theme	depressive-like	416:430	arg1	behavior					432:439	LPS-induced depressive-like behavior	404:439	LPS-induced depressive-like behavior	404:439	In this study, we further investigated the role of GluN2 A subunit of NMDA receptor in synaptic processes in the prefrontal cortex (PFC) and hippocampus of GluN2 A knockout (KO) mice in LPS-induced depressive-like behavior.
30296532	6	33	theme	increased	958:966	arg1	levels					968:973	increased levels	958:973	increased levels of all NCAM isoforms in PFC upon LPS which was followed with a decrease in GluN1 and GluN2B subunits	958:1074	Furthermore, the absence of GluN2 A resulted in increased levels of all NCAM isoforms in PFC upon LPS which was followed with a decrease in GluN1 and GluN2B subunits.
30296532	7	34	from	present	1224:1230	arg1	PFC					1239:1241	the PFC	1235:1241	the PFC of KO mice	1235:1252	The levels of AMPA receptor subunits (GluA1, GluA3, and GluA4) in the hippocampus of GluN2 A mice were unaltered upon the treatment and abundantly present in the PFC of KO mice.
30296532	2	35	theme	synaptic	305:312	arg1	processes					314:322	synaptic processes	305:322	synaptic processes in the prefrontal cortex (PFC) and hippocampus of GluN2 A knockout (KO) mice in LPS-induced depressive-like behavior	305:439	In this study, we further investigated the role of GluN2 A subunit of NMDA receptor in synaptic processes in the prefrontal cortex (PFC) and hippocampus of GluN2 A knockout (KO) mice in LPS-induced depressive-like behavior.
30296532	6	36	from	decrease	1038:1045	arg1	GluN2B					1060:1065	GluN2B	1060:1065	GluN2B	1060:1065	Furthermore, the absence of GluN2 A resulted in increased levels of all NCAM isoforms in PFC upon LPS which was followed with a decrease in GluN1 and GluN2B subunits.
30296532	6	36	from	decrease	1038:1045	arg1	GluN1					1050:1054	GluN1	1050:1054	GluN1	1050:1054	Furthermore, the absence of GluN2 A resulted in increased levels of all NCAM isoforms in PFC upon LPS which was followed with a decrease in GluN1 and GluN2B subunits.
30296532	4	37	theme	neuroplasticity	626:640	arg1	markers					642:648	neuroplasticity markers	626:648	neuroplasticity markers	626:648	This was accompanied by unaltered levels of IL-6 and significant changes in neuroplasticity markers and glutamate receptor subunits composition in PFC and hippocampus.
30296532	2	38	theme	LPS-induced	404:414	arg1	behavior					432:439	LPS-induced depressive-like behavior	404:439	LPS-induced depressive-like behavior	404:439	In this study, we further investigated the role of GluN2 A subunit of NMDA receptor in synaptic processes in the prefrontal cortex (PFC) and hippocampus of GluN2 A knockout (KO) mice in LPS-induced depressive-like behavior.
30296532	5	39	from	challenge	743:751	arg1	mice					767:770	GluN2 A KO mice	756:770	GluN2 A KO mice	756:770	In particular, an immune challenge in GluN2 A KO mice resulted in unchanged PSA-NCAM levels and proBDNF increase in both brain structures as well as in increase in BDNF levels in hippocampus.
30296532	7	40	theme	KO	1246:1247	arg1	mice					1249:1252	KO mice	1246:1252	KO mice	1246:1252	The levels of AMPA receptor subunits (GluA1, GluA3, and GluA4) in the hippocampus of GluN2 A mice were unaltered upon the treatment and abundantly present in the PFC of KO mice.
30296532	2	41	theme	mice	396:399	arg1	cortex					342:347	the prefrontal cortex	327:347	the prefrontal cortex (PFC)	327:353	In this study, we further investigated the role of GluN2 A subunit of NMDA receptor in synaptic processes in the prefrontal cortex (PFC) and hippocampus of GluN2 A knockout (KO) mice in LPS-induced depressive-like behavior.
30296532	2	41	theme	mice	396:399	arg1	PFC					350:352	PFC	350:352	PFC	350:352	In this study, we further investigated the role of GluN2 A subunit of NMDA receptor in synaptic processes in the prefrontal cortex (PFC) and hippocampus of GluN2 A knockout (KO) mice in LPS-induced depressive-like behavior.
30296532	2	41	theme	mice	396:399	arg1	hippocampus					359:369	hippocampus	359:369	hippocampus of GluN2 A knockout (KO) mice in LPS-induced depressive-like behavior	359:439	In this study, we further investigated the role of GluN2 A subunit of NMDA receptor in synaptic processes in the prefrontal cortex (PFC) and hippocampus of GluN2 A knockout (KO) mice in LPS-induced depressive-like behavior.
30296532	0	42	theme	GluN2A	32:37	arg1	subunit					39:45	the NMDA receptor GluN2A subunit	14:45	the NMDA receptor GluN2A subunit	14:45	Disruption of the NMDA receptor GluN2A subunit abolishes inflammation-induced depression.
30296532	4	43	from	subunits	673:680	arg1	markers					642:648	neuroplasticity markers	626:648	neuroplasticity markers	626:648	This was accompanied by unaltered levels of IL-6 and significant changes in neuroplasticity markers and glutamate receptor subunits composition in PFC and hippocampus.
30296532	4	43	from	subunits	673:680	arg1	hippocampus					705:715	hippocampus	705:715	hippocampus	705:715	This was accompanied by unaltered levels of IL-6 and significant changes in neuroplasticity markers and glutamate receptor subunits composition in PFC and hippocampus.
30296532	4	43	from	subunits	673:680	arg1	PFC					697:699	PFC	697:699	PFC	697:699	This was accompanied by unaltered levels of IL-6 and significant changes in neuroplasticity markers and glutamate receptor subunits composition in PFC and hippocampus.
30296532	6	44	from	levels	968:973	arg1	PFC					999:1001	PFC	999:1001	PFC upon LPS	999:1010	Furthermore, the absence of GluN2 A resulted in increased levels of all NCAM isoforms in PFC upon LPS which was followed with a decrease in GluN1 and GluN2B subunits.
30296532	8	45	theme	LPS-induced	1383:1393	arg1	phenotype					1406:1414	LPS-induced depressive phenotype	1383:1414	LPS-induced depressive phenotype	1383:1414	These results indicate that the GluN2 A subunit is critical in neuroinflammation-related depression, that its absence abolishes LPS-induced depressive phenotype, sustains PSA-NCAM levels, increases proBDNF signalling in the PFC and hippocampus and potentiates synaptic stabilization through NCAM in the PFC upon an immune challenge.
30296532	8	46	theme	neuroinflammation-related	1318:1342	arg1	depression					1344:1353	neuroinflammation-related depression	1318:1353	neuroinflammation-related depression	1318:1353	These results indicate that the GluN2 A subunit is critical in neuroinflammation-related depression, that its absence abolishes LPS-induced depressive phenotype, sustains PSA-NCAM levels, increases proBDNF signalling in the PFC and hippocampus and potentiates synaptic stabilization through NCAM in the PFC upon an immune challenge.
30296532	8	47	from	depression	1344:1353	arg1	subunit					1295:1301	the GluN2 A subunit	1283:1301	the GluN2 A subunit	1283:1301	These results indicate that the GluN2 A subunit is critical in neuroinflammation-related depression, that its absence abolishes LPS-induced depressive phenotype, sustains PSA-NCAM levels, increases proBDNF signalling in the PFC and hippocampus and potentiates synaptic stabilization through NCAM in the PFC upon an immune challenge.
30296532	8	47	from	depression	1344:1353	arg1	critical					1306:1313	critical	1306:1313	critical	1306:1313	These results indicate that the GluN2 A subunit is critical in neuroinflammation-related depression, that its absence abolishes LPS-induced depressive phenotype, sustains PSA-NCAM levels, increases proBDNF signalling in the PFC and hippocampus and potentiates synaptic stabilization through NCAM in the PFC upon an immune challenge.
30296532	7	48	theme	receptor	1096:1103	arg1	GluA3					1122:1126	GluA3	1122:1126	GluA3	1122:1126	The levels of AMPA receptor subunits (GluA1, GluA3, and GluA4) in the hippocampus of GluN2 A mice were unaltered upon the treatment and abundantly present in the PFC of KO mice.
30296532	7	48	theme	receptor	1096:1103	arg1	GluA4					1133:1137	GluA4	1133:1137	GluA4	1133:1137	The levels of AMPA receptor subunits (GluA1, GluA3, and GluA4) in the hippocampus of GluN2 A mice were unaltered upon the treatment and abundantly present in the PFC of KO mice.
30296532	7	48	theme	receptor	1096:1103	arg1	GluA1					1115:1119	GluA1	1115:1119	GluA1	1115:1119	The levels of AMPA receptor subunits (GluA1, GluA3, and GluA4) in the hippocampus of GluN2 A mice were unaltered upon the treatment and abundantly present in the PFC of KO mice.
30296532	7	48	theme	receptor	1096:1103	arg1	subunits					1105:1112	AMPA receptor subunits	1091:1112	AMPA receptor subunits (GluA1, GluA3, and GluA4)	1091:1138	The levels of AMPA receptor subunits (GluA1, GluA3, and GluA4) in the hippocampus of GluN2 A mice were unaltered upon the treatment and abundantly present in the PFC of KO mice.
30296532	0	49	theme	receptor	23:30	arg1	subunit					39:45	the NMDA receptor GluN2A subunit	14:45	the NMDA receptor GluN2A subunit	14:45	Disruption of the NMDA receptor GluN2A subunit abolishes inflammation-induced depression.
30296532	6	50	theme	GluN2 A	938:944	arg1	absence					927:933	the absence	923:933	the absence of GluN2 A	923:944	Furthermore, the absence of GluN2 A resulted in increased levels of all NCAM isoforms in PFC upon LPS which was followed with a decrease in GluN1 and GluN2B subunits.
30296532	5	51	theme	unchanged	784:792	arg1	levels					803:808	unchanged PSA-NCAM levels	784:808	unchanged PSA-NCAM levels	784:808	In particular, an immune challenge in GluN2 A KO mice resulted in unchanged PSA-NCAM levels and proBDNF increase in both brain structures as well as in increase in BDNF levels in hippocampus.
30296532	5	52	theme	immune	736:741	arg1	challenge					743:751	an immune challenge	733:751	an immune challenge in GluN2 A KO mice	733:770	In particular, an immune challenge in GluN2 A KO mice resulted in unchanged PSA-NCAM levels and proBDNF increase in both brain structures as well as in increase in BDNF levels in hippocampus.
30296532	0	53	theme	NMDA	18:21	arg1	subunit					39:45	the NMDA receptor GluN2A subunit	14:45	the NMDA receptor GluN2A subunit	14:45	Disruption of the NMDA receptor GluN2A subunit abolishes inflammation-induced depression.
30296532	2	54	theme	KO	392:393	arg1	mice					396:399	GluN2 A knockout (KO) mice	374:399	GluN2 A knockout (KO) mice	374:399	In this study, we further investigated the role of GluN2 A subunit of NMDA receptor in synaptic processes in the prefrontal cortex (PFC) and hippocampus of GluN2 A knockout (KO) mice in LPS-induced depressive-like behavior.
30296532	5	55	theme	PSA-NCAM	794:801	arg1	levels					803:808	unchanged PSA-NCAM levels	784:808	unchanged PSA-NCAM levels	784:808	In particular, an immune challenge in GluN2 A KO mice resulted in unchanged PSA-NCAM levels and proBDNF increase in both brain structures as well as in increase in BDNF levels in hippocampus.
30296532	7	56	from	levels	1081:1086	arg1	hippocampus					1147:1157	the hippocampus	1143:1157	the hippocampus of GluN2 A mice	1143:1173	The levels of AMPA receptor subunits (GluA1, GluA3, and GluA4) in the hippocampus of GluN2 A mice were unaltered upon the treatment and abundantly present in the PFC of KO mice.
30296532	5	57	theme	KO	764:765	arg1	mice					767:770	GluN2 A KO mice	756:770	GluN2 A KO mice	756:770	In particular, an immune challenge in GluN2 A KO mice resulted in unchanged PSA-NCAM levels and proBDNF increase in both brain structures as well as in increase in BDNF levels in hippocampus.
30296532	8	58	theme	GluN2 A	1287:1293	arg1	subunit					1295:1301	the GluN2 A subunit	1283:1301	the GluN2 A subunit	1283:1301	These results indicate that the GluN2 A subunit is critical in neuroinflammation-related depression, that its absence abolishes LPS-induced depressive phenotype, sustains PSA-NCAM levels, increases proBDNF signalling in the PFC and hippocampus and potentiates synaptic stabilization through NCAM in the PFC upon an immune challenge.
30296532	8	58	theme	GluN2 A	1287:1293	arg1	critical					1306:1313	critical	1306:1313	critical	1306:1313	These results indicate that the GluN2 A subunit is critical in neuroinflammation-related depression, that its absence abolishes LPS-induced depressive phenotype, sustains PSA-NCAM levels, increases proBDNF signalling in the PFC and hippocampus and potentiates synaptic stabilization through NCAM in the PFC upon an immune challenge.
30296532	0	59	theme	subunit	39:45	arg1	Disruption					0:9	Disruption	0:9	Disruption of the NMDA receptor GluN2A subunit	0:45	Disruption of the NMDA receptor GluN2A subunit abolishes inflammation-induced depression.
30296532	8	60	theme	PSA-NCAM	1426:1433	arg1	levels					1435:1440	PSA-NCAM levels	1426:1440	PSA-NCAM levels	1426:1440	These results indicate that the GluN2 A subunit is critical in neuroinflammation-related depression, that its absence abolishes LPS-induced depressive phenotype, sustains PSA-NCAM levels, increases proBDNF signalling in the PFC and hippocampus and potentiates synaptic stabilization through NCAM in the PFC upon an immune challenge.
30296532	7	61	theme	GluN2 A	1162:1168	arg1	mice					1170:1173	GluN2 A mice	1162:1173	GluN2 A mice	1162:1173	The levels of AMPA receptor subunits (GluA1, GluA3, and GluA4) in the hippocampus of GluN2 A mice were unaltered upon the treatment and abundantly present in the PFC of KO mice.
30296532	4	62	dep	changes	615:621	arg1	composition					682:692	composition	682:692	composition	682:692	This was accompanied by unaltered levels of IL-6 and significant changes in neuroplasticity markers and glutamate receptor subunits composition in PFC and hippocampus.
30296532	7	63	dep	subunits	1105:1112	arg1	GluA3					1122:1126	GluA3	1122:1126	GluA3	1122:1126	The levels of AMPA receptor subunits (GluA1, GluA3, and GluA4) in the hippocampus of GluN2 A mice were unaltered upon the treatment and abundantly present in the PFC of KO mice.
30296532	7	63	dep	subunits	1105:1112	arg1	GluA4					1133:1137	GluA4	1133:1137	GluA4	1133:1137	The levels of AMPA receptor subunits (GluA1, GluA3, and GluA4) in the hippocampus of GluN2 A mice were unaltered upon the treatment and abundantly present in the PFC of KO mice.
30296532	7	63	dep	subunits	1105:1112	arg1	GluA1					1115:1119	GluA1	1115:1119	GluA1	1115:1119	The levels of AMPA receptor subunits (GluA1, GluA3, and GluA4) in the hippocampus of GluN2 A mice were unaltered upon the treatment and abundantly present in the PFC of KO mice.
30296532	7	63	dep	subunits	1105:1112	arg1	subunits					1105:1112	AMPA receptor subunits	1091:1112	AMPA receptor subunits (GluA1, GluA3, and GluA4)	1091:1138	The levels of AMPA receptor subunits (GluA1, GluA3, and GluA4) in the hippocampus of GluN2 A mice were unaltered upon the treatment and abundantly present in the PFC of KO mice.
30296532	7	64	from	PFC	1239:1241	arg1	levels					1081:1086	The levels	1077:1086	The levels of AMPA receptor subunits (GluA1, GluA3, and GluA4) in the hippocampus of GluN2 A mice	1077:1173	The levels of AMPA receptor subunits (GluA1, GluA3, and GluA4) in the hippocampus of GluN2 A mice were unaltered upon the treatment and abundantly present in the PFC of KO mice.
30296532	7	64	from	PFC	1239:1241	arg1	unaltered					1180:1188	unaltered	1180:1188	unaltered	1180:1188	The levels of AMPA receptor subunits (GluA1, GluA3, and GluA4) in the hippocampus of GluN2 A mice were unaltered upon the treatment and abundantly present in the PFC of KO mice.
30296532	7	64	from	PFC	1239:1241	arg1	present					1224:1230	present	1224:1230	present	1224:1230	The levels of AMPA receptor subunits (GluA1, GluA3, and GluA4) in the hippocampus of GluN2 A mice were unaltered upon the treatment and abundantly present in the PFC of KO mice.
30296532	2	65	theme	GluN2 A	269:275	arg1	subunit					277:283	GluN2 A subunit	269:283	GluN2 A subunit of NMDA receptor	269:300	In this study, we further investigated the role of GluN2 A subunit of NMDA receptor in synaptic processes in the prefrontal cortex (PFC) and hippocampus of GluN2 A knockout (KO) mice in LPS-induced depressive-like behavior.
30296532	2	66	theme	prefrontal	331:340	arg1	cortex					342:347	the prefrontal cortex	327:347	the prefrontal cortex (PFC)	327:353	In this study, we further investigated the role of GluN2 A subunit of NMDA receptor in synaptic processes in the prefrontal cortex (PFC) and hippocampus of GluN2 A knockout (KO) mice in LPS-induced depressive-like behavior.
30296532	2	66	theme	prefrontal	331:340	arg1	PFC					350:352	PFC	350:352	PFC	350:352	In this study, we further investigated the role of GluN2 A subunit of NMDA receptor in synaptic processes in the prefrontal cortex (PFC) and hippocampus of GluN2 A knockout (KO) mice in LPS-induced depressive-like behavior.
30296532	6	67	dep	GluN1	1050:1054	arg1	subunits					1067:1074	subunits	1067:1074	subunits	1067:1074	Furthermore, the absence of GluN2 A resulted in increased levels of all NCAM isoforms in PFC upon LPS which was followed with a decrease in GluN1 and GluN2B subunits.
30296532	2	68	theme	subunit	277:283	arg1	role					261:264	the role	257:264	the role of GluN2 A subunit of NMDA receptor in synaptic processes in the prefrontal cortex (PFC) and hippocampus of GluN2 A knockout (KO) mice in LPS-induced depressive-like behavior	257:439	In this study, we further investigated the role of GluN2 A subunit of NMDA receptor in synaptic processes in the prefrontal cortex (PFC) and hippocampus of GluN2 A knockout (KO) mice in LPS-induced depressive-like behavior.
30296532	8	69	theme	depressive	1395:1404	arg1	phenotype					1406:1414	LPS-induced depressive phenotype	1383:1414	LPS-induced depressive phenotype	1383:1414	These results indicate that the GluN2 A subunit is critical in neuroinflammation-related depression, that its absence abolishes LPS-induced depressive phenotype, sustains PSA-NCAM levels, increases proBDNF signalling in the PFC and hippocampus and potentiates synaptic stabilization through NCAM in the PFC upon an immune challenge.
30296532	3	70	theme	depressive-like	523:537	arg1	behaviour					539:547	depressive-like behaviour	523:547	depressive-like behaviour	523:547	Our data suggest that LPS-treated mice, lacking GluN2 A subunit, did not exhibit depressive-like behaviour.
30296532	5	71	theme	BDNF	882:885	arg1	levels					887:892	BDNF levels	882:892	BDNF levels in hippocampus	882:907	In particular, an immune challenge in GluN2 A KO mice resulted in unchanged PSA-NCAM levels and proBDNF increase in both brain structures as well as in increase in BDNF levels in hippocampus.
30296532	3	72	theme	LPS-treated	464:474	arg1	mice					476:479	LPS-treated mice	464:479	LPS-treated mice	464:479	Our data suggest that LPS-treated mice, lacking GluN2 A subunit, did not exhibit depressive-like behaviour.
30296532	4	73	from	changes	615:621	arg1	markers					642:648	neuroplasticity markers	626:648	neuroplasticity markers	626:648	This was accompanied by unaltered levels of IL-6 and significant changes in neuroplasticity markers and glutamate receptor subunits composition in PFC and hippocampus.
30296532	4	73	from	changes	615:621	arg1	hippocampus					705:715	hippocampus	705:715	hippocampus	705:715	This was accompanied by unaltered levels of IL-6 and significant changes in neuroplasticity markers and glutamate receptor subunits composition in PFC and hippocampus.
30296532	4	73	from	changes	615:621	arg1	PFC					697:699	PFC	697:699	PFC	697:699	This was accompanied by unaltered levels of IL-6 and significant changes in neuroplasticity markers and glutamate receptor subunits composition in PFC and hippocampus.
30296532	5	74	from	increase	870:877	arg1	levels					887:892	BDNF levels	882:892	BDNF levels in hippocampus	882:907	In particular, an immune challenge in GluN2 A KO mice resulted in unchanged PSA-NCAM levels and proBDNF increase in both brain structures as well as in increase in BDNF levels in hippocampus.
30296532	1	75	theme	-induced	152:159	arg1	behaviour					177:185	lipopolysaccharide (LPS)-induced depressive-like behaviour	128:185	lipopolysaccharide (LPS)-induced depressive-like behaviour	128:185	Recent reports have demonstrated that lipopolysaccharide (LPS)-induced depressive-like behaviour is mediated via NMDA receptor.
30296532	4	76	theme	glutamate	654:662	arg1	subunits					673:680	glutamate receptor subunits	654:680	glutamate receptor subunits	654:680	This was accompanied by unaltered levels of IL-6 and significant changes in neuroplasticity markers and glutamate receptor subunits composition in PFC and hippocampus.
30296532	8	77	theme	immune	1570:1575	arg1	challenge					1577:1585	an immune challenge	1567:1585	an immune challenge	1567:1585	These results indicate that the GluN2 A subunit is critical in neuroinflammation-related depression, that its absence abolishes LPS-induced depressive phenotype, sustains PSA-NCAM levels, increases proBDNF signalling in the PFC and hippocampus and potentiates synaptic stabilization through NCAM in the PFC upon an immune challenge.
30296532	0	78	theme	inflammation-induced	57:76	arg1	depression					78:87	inflammation-induced depression	57:87	inflammation-induced depression	57:87	Disruption of the NMDA receptor GluN2A subunit abolishes inflammation-induced depression.
30296532	8	79	theme	proBDNF	1453:1459	arg1	signalling					1461:1470	proBDNF signalling	1453:1470	proBDNF signalling in the PFC and hippocampus	1453:1497	These results indicate that the GluN2 A subunit is critical in neuroinflammation-related depression, that its absence abolishes LPS-induced depressive phenotype, sustains PSA-NCAM levels, increases proBDNF signalling in the PFC and hippocampus and potentiates synaptic stabilization through NCAM in the PFC upon an immune challenge.
30296532	4	80	theme	IL-6	594:597	arg1	levels					584:589	unaltered levels	574:589	unaltered levels of IL-6	574:597	This was accompanied by unaltered levels of IL-6 and significant changes in neuroplasticity markers and glutamate receptor subunits composition in PFC and hippocampus.
30296532	4	80	theme	IL-6	594:597	arg1	subunits					673:680	glutamate receptor subunits	654:680	glutamate receptor subunits	654:680	This was accompanied by unaltered levels of IL-6 and significant changes in neuroplasticity markers and glutamate receptor subunits composition in PFC and hippocampus.
30296532	4	80	theme	IL-6	594:597	arg1	changes					615:621	significant changes	603:621	significant changes in neuroplasticity markers	603:648	This was accompanied by unaltered levels of IL-6 and significant changes in neuroplasticity markers and glutamate receptor subunits composition in PFC and hippocampus.
30296532	1	81	theme	depressive-like	161:175	arg1	behaviour					177:185	lipopolysaccharide (LPS)-induced depressive-like behaviour	128:185	lipopolysaccharide (LPS)-induced depressive-like behaviour	128:185	Recent reports have demonstrated that lipopolysaccharide (LPS)-induced depressive-like behaviour is mediated via NMDA receptor.
30296532	8	82	from	critical	1306:1313	arg1	depression					1344:1353	neuroinflammation-related depression	1318:1353	neuroinflammation-related depression	1318:1353	These results indicate that the GluN2 A subunit is critical in neuroinflammation-related depression, that its absence abolishes LPS-induced depressive phenotype, sustains PSA-NCAM levels, increases proBDNF signalling in the PFC and hippocampus and potentiates synaptic stabilization through NCAM in the PFC upon an immune challenge.
30296532	4	83	theme	significant	603:613	arg1	changes					615:621	significant changes	603:621	significant changes in neuroplasticity markers	603:648	This was accompanied by unaltered levels of IL-6 and significant changes in neuroplasticity markers and glutamate receptor subunits composition in PFC and hippocampus.
29580737	4	0	theme	decoy	738:742	arg1	database					751:758	a decoy glycan database	736:758	a decoy glycan database based on the glycan structures in the target database	736:812	In this manuscript, we introduced a novel method that constructed a decoy glycan database based on the glycan structures in the target database, and searched the experimental spectra against both the target and decoy databases to find the best matched glycans.
29580737	2	1	theme	glycans	369:375	arg1	characterization					349:364	the characterization	345:364	the characterization of glycans and glycopeptides	345:393	Currently, appropriate sample preparation followed by tandem mass spectrometry has emerged as the dominant technique for the characterization of glycans and glycopeptides.
29580737	0	2	theme	target-decoy	69:80	arg1	strategy					82:89	target-decoy strategy	69:89	target-decoy strategy	69:89	An approach for N-linked glycan identification from MS/MS spectra by target-decoy strategy.
29580737	0	3	link	N-linked	16:23	arg1	identification					32:45	N-linked glycan identification	16:45	N-linked glycan identification	16:45	An approach for N-linked glycan identification from MS/MS spectra by target-decoy strategy.
29580737	1	4	theme	thorough	159:166	arg1	investigation					168:180	the thorough investigation	155:180	the thorough investigation of the structure and function of protein	155:221	Glycan structure determination serves as an essential step for the thorough investigation of the structure and function of protein.
29580737	5	5	theme	search	1034:1039	arg1	procedure					1041:1049	the search procedure	1030:1049	the search procedure which enables the unbiased ranking of the matched glycans	1030:1107	Specifically, a two-layer scoring scheme for calculating a normalized matching score is applied in the search procedure which enables the unbiased ranking of the matched glycans.
29580737	4	6	theme	target	798:803	arg1	database					805:812	the target database	794:812	the target database	794:812	In this manuscript, we introduced a novel method that constructed a decoy glycan database based on the glycan structures in the target database, and searched the experimental spectra against both the target and decoy databases to find the best matched glycans.
29580737	6	7	theme	more	1175:1178	arg1	structures					1180:1189	more structures	1175:1189	more structures	1175:1189	Experimental analysis showed that our proposed method can report more structures with high confidence compared with previous approaches.
29580737	4	8	theme	glycan	773:778	arg1	structures					780:789	the glycan structures	769:789	the glycan structures in the target database	769:812	In this manuscript, we introduced a novel method that constructed a decoy glycan database based on the glycan structures in the target database, and searched the experimental spectra against both the target and decoy databases to find the best matched glycans.
29580737	5	9	theme	matching	1001:1008	arg1	score					1010:1014	a normalized matching score	988:1014	a normalized matching score	988:1014	Specifically, a two-layer scoring scheme for calculating a normalized matching score is applied in the search procedure which enables the unbiased ranking of the matched glycans.
29580737	3	10	theme	reasonable	582:591	arg1	strategy					609:616	a reasonable quality control strategy	580:616	a reasonable quality control strategy for the statistical validation of reported results	580:667	Although extensive efforts have been made to the development of computational approaches for the automated interpretation of glycopeptide spectra, the previously appeared methods lack a reasonable quality control strategy for the statistical validation of reported results.
29580737	5	11	theme	scoring	957:963	arg1	scheme					965:970	a two-layer scoring scheme	945:970	a two-layer scoring scheme for calculating a normalized matching score	945:1014	Specifically, a two-layer scoring scheme for calculating a normalized matching score is applied in the search procedure which enables the unbiased ranking of the matched glycans.
29580737	3	12	theme	automated	493:501	arg1	interpretation					503:516	the automated interpretation	489:516	the automated interpretation of glycopeptide spectra	489:540	Although extensive efforts have been made to the development of computational approaches for the automated interpretation of glycopeptide spectra, the previously appeared methods lack a reasonable quality control strategy for the statistical validation of reported results.
29580737	2	13	theme	mass	285:288	arg1	spectrometry					290:301	tandem mass spectrometry	278:301	tandem mass spectrometry	278:301	Currently, appropriate sample preparation followed by tandem mass spectrometry has emerged as the dominant technique for the characterization of glycans and glycopeptides.
29580737	6	14	theme	Experimental	1110:1121	arg1	analysis					1123:1130	Experimental analysis	1110:1130	Experimental analysis	1110:1130	Experimental analysis showed that our proposed method can report more structures with high confidence compared with previous approaches.
29580737	4	15	theme	experimental	832:843	arg1	spectra					845:851	the experimental spectra	828:851	the experimental spectra against both the target and decoy databases	828:895	In this manuscript, we introduced a novel method that constructed a decoy glycan database based on the glycan structures in the target database, and searched the experimental spectra against both the target and decoy databases to find the best matched glycans.
29580737	2	16	theme	tandem	278:283	arg1	spectrometry					290:301	tandem mass spectrometry	278:301	tandem mass spectrometry	278:301	Currently, appropriate sample preparation followed by tandem mass spectrometry has emerged as the dominant technique for the characterization of glycans and glycopeptides.
29580737	3	17	theme	computational	460:472	arg1	approaches					474:483	computational approaches	460:483	computational approaches for the automated interpretation of glycopeptide spectra	460:540	Although extensive efforts have been made to the development of computational approaches for the automated interpretation of glycopeptide spectra, the previously appeared methods lack a reasonable quality control strategy for the statistical validation of reported results.
29580737	3	18	theme	extensive	405:413	arg1	efforts					415:421	extensive efforts	405:421	extensive efforts	405:421	Although extensive efforts have been made to the development of computational approaches for the automated interpretation of glycopeptide spectra, the previously appeared methods lack a reasonable quality control strategy for the statistical validation of reported results.
29580737	6	19	theme	high	1196:1199	arg1	confidence					1201:1210	high confidence	1196:1210	high confidence	1196:1210	Experimental analysis showed that our proposed method can report more structures with high confidence compared with previous approaches.
29580737	4	20	from	structures	780:789	arg1	database					805:812	the target database	794:812	the target database	794:812	In this manuscript, we introduced a novel method that constructed a decoy glycan database based on the glycan structures in the target database, and searched the experimental spectra against both the target and decoy databases to find the best matched glycans.
29580737	2	21	theme	dominant	322:329	arg1	technique					331:339	the dominant technique	318:339	the dominant technique for the characterization of glycans and glycopeptides	318:393	Currently, appropriate sample preparation followed by tandem mass spectrometry has emerged as the dominant technique for the characterization of glycans and glycopeptides.
29580737	4	22	theme	decoy	881:885	arg1	databases					887:895	both the target and decoy databases	861:895	databases	887:895	In this manuscript, we introduced a novel method that constructed a decoy glycan database based on the glycan structures in the target database, and searched the experimental spectra against both the target and decoy databases to find the best matched glycans.
29580737	1	23	theme	Glycan	92:97	arg1	step					146:149	an essential step	133:149	an essential step for the thorough investigation of the structure and function of protein	133:221	Glycan structure determination serves as an essential step for the thorough investigation of the structure and function of protein.
29580737	1	23	theme	Glycan	92:97	arg1	determination					109:121	Glycan structure determination	92:121	Glycan structure determination	92:121	Glycan structure determination serves as an essential step for the thorough investigation of the structure and function of protein.
29580737	1	24	theme	structure	189:197	arg1	investigation					168:180	the thorough investigation	155:180	the thorough investigation of the structure and function of protein	155:221	Glycan structure determination serves as an essential step for the thorough investigation of the structure and function of protein.
29580737	0	25	theme	N-linked	16:23	arg1	identification					32:45	N-linked glycan identification	16:45	N-linked glycan identification	16:45	An approach for N-linked glycan identification from MS/MS spectra by target-decoy strategy.
29580737	3	26	theme	reported	652:659	arg1	results					661:667	reported results	652:667	reported results	652:667	Although extensive efforts have been made to the development of computational approaches for the automated interpretation of glycopeptide spectra, the previously appeared methods lack a reasonable quality control strategy for the statistical validation of reported results.
29580737	1	27	theme	structure	99:107	arg1	step					146:149	an essential step	133:149	an essential step for the thorough investigation of the structure and function of protein	133:221	Glycan structure determination serves as an essential step for the thorough investigation of the structure and function of protein.
29580737	1	27	theme	structure	99:107	arg1	determination					109:121	Glycan structure determination	92:121	Glycan structure determination	92:121	Glycan structure determination serves as an essential step for the thorough investigation of the structure and function of protein.
29580737	3	28	gly	glycopeptide	521:532	arg2	glycopeptide					521:532	glycopeptide spectra	521:540	glycopeptide spectra	521:540	Although extensive efforts have been made to the development of computational approaches for the automated interpretation of glycopeptide spectra, the previously appeared methods lack a reasonable quality control strategy for the statistical validation of reported results.
29580737	6	29	theme	previous	1226:1233	arg1	approaches					1235:1244	previous approaches	1226:1244	previous approaches	1226:1244	Experimental analysis showed that our proposed method can report more structures with high confidence compared with previous approaches.
29580737	2	30	gly	glycopeptides	381:393	arg2	glycopeptides					381:393	glycopeptides	381:393	glycopeptides	381:393	Currently, appropriate sample preparation followed by tandem mass spectrometry has emerged as the dominant technique for the characterization of glycans and glycopeptides.
29580737	5	31	theme	matched	1093:1099	arg1	glycans					1101:1107	the matched glycans	1089:1107	the matched glycans	1089:1107	Specifically, a two-layer scoring scheme for calculating a normalized matching score is applied in the search procedure which enables the unbiased ranking of the matched glycans.
29580737	3	32	theme	control	601:607	arg1	strategy					609:616	a reasonable quality control strategy	580:616	a reasonable quality control strategy for the statistical validation of reported results	580:667	Although extensive efforts have been made to the development of computational approaches for the automated interpretation of glycopeptide spectra, the previously appeared methods lack a reasonable quality control strategy for the statistical validation of reported results.
29580737	1	33	theme	function	203:210	arg1	investigation					168:180	the thorough investigation	155:180	the thorough investigation of the structure and function of protein	155:221	Glycan structure determination serves as an essential step for the thorough investigation of the structure and function of protein.
29580737	4	34	theme	glycan	744:749	arg1	database					751:758	a decoy glycan database	736:758	a decoy glycan database based on the glycan structures in the target database	736:812	In this manuscript, we introduced a novel method that constructed a decoy glycan database based on the glycan structures in the target database, and searched the experimental spectra against both the target and decoy databases to find the best matched glycans.
29580737	5	35	theme	glycans	1101:1107	arg1	ranking					1078:1084	the unbiased ranking	1065:1084	the unbiased ranking of the matched glycans	1065:1107	Specifically, a two-layer scoring scheme for calculating a normalized matching score is applied in the search procedure which enables the unbiased ranking of the matched glycans.
29580737	2	36	theme	appropriate	235:245	arg1	preparation					254:264	appropriate sample preparation	235:264	appropriate sample preparation followed by tandem mass spectrometry	235:301	Currently, appropriate sample preparation followed by tandem mass spectrometry has emerged as the dominant technique for the characterization of glycans and glycopeptides.
29580737	4	37	theme	novel	706:710	arg1	method					712:717	a novel method	704:717	a novel method that constructed a decoy glycan database based on the glycan structures in the target database, and searched the experimental spectra against both the target and decoy databases to find the best matched glycans	704:928	In this manuscript, we introduced a novel method that constructed a decoy glycan database based on the glycan structures in the target database, and searched the experimental spectra against both the target and decoy databases to find the best matched glycans.
29580737	3	38	theme	approaches	474:483	arg1	development					445:455	the development	441:455	the development of computational approaches for the automated interpretation of glycopeptide spectra	441:540	Although extensive efforts have been made to the development of computational approaches for the automated interpretation of glycopeptide spectra, the previously appeared methods lack a reasonable quality control strategy for the statistical validation of reported results.
29580737	0	39	theme	glycan	25:30	arg1	identification					32:45	N-linked glycan identification	16:45	N-linked glycan identification	16:45	An approach for N-linked glycan identification from MS/MS spectra by target-decoy strategy.
29580737	1	40	dep	structure	189:197	arg1	the					185:187	the	185:187	the	185:187	Glycan structure determination serves as an essential step for the thorough investigation of the structure and function of protein.
29580737	1	41	theme	protein	215:221	arg1	function					203:210	function	203:210	function	203:210	Glycan structure determination serves as an essential step for the thorough investigation of the structure and function of protein.
29580737	1	41	theme	protein	215:221	arg1	structure					189:197	structure	189:197	structure	189:197	Glycan structure determination serves as an essential step for the thorough investigation of the structure and function of protein.
29580737	0	42	theme	MS/MS	52:56	arg1	spectra					58:64	MS/MS spectra	52:64	MS/MS spectra	52:64	An approach for N-linked glycan identification from MS/MS spectra by target-decoy strategy.
29580737	0	43	from	spectra	58:64	arg1	approach					3:10	An approach	0:10	An approach for N-linked glycan identification from MS/MS spectra	0:64	An approach for N-linked glycan identification from MS/MS spectra by target-decoy strategy.
29580737	3	44	theme	glycopeptide	521:532	arg1	spectra					534:540	glycopeptide spectra	521:540	glycopeptide spectra	521:540	Although extensive efforts have been made to the development of computational approaches for the automated interpretation of glycopeptide spectra, the previously appeared methods lack a reasonable quality control strategy for the statistical validation of reported results.
29580737	4	45	theme	matched	914:920	arg1	glycans					922:928	the best matched glycans	905:928	the best matched glycans	905:928	In this manuscript, we introduced a novel method that constructed a decoy glycan database based on the glycan structures in the target database, and searched the experimental spectra against both the target and decoy databases to find the best matched glycans.
29580737	5	46	theme	unbiased	1069:1076	arg1	ranking					1078:1084	the unbiased ranking	1065:1084	the unbiased ranking of the matched glycans	1065:1107	Specifically, a two-layer scoring scheme for calculating a normalized matching score is applied in the search procedure which enables the unbiased ranking of the matched glycans.
29580737	3	47	theme	statistical	626:636	arg1	validation					638:647	the statistical validation	622:647	the statistical validation of reported results	622:667	Although extensive efforts have been made to the development of computational approaches for the automated interpretation of glycopeptide spectra, the previously appeared methods lack a reasonable quality control strategy for the statistical validation of reported results.
29580737	3	48	theme	spectra	534:540	arg1	interpretation					503:516	the automated interpretation	489:516	the automated interpretation of glycopeptide spectra	489:540	Although extensive efforts have been made to the development of computational approaches for the automated interpretation of glycopeptide spectra, the previously appeared methods lack a reasonable quality control strategy for the statistical validation of reported results.
29580737	3	49	theme	results	661:667	arg1	validation					638:647	the statistical validation	622:647	the statistical validation of reported results	622:667	Although extensive efforts have been made to the development of computational approaches for the automated interpretation of glycopeptide spectra, the previously appeared methods lack a reasonable quality control strategy for the statistical validation of reported results.
29580737	5	50	theme	two-layer	947:955	arg1	scheme					965:970	a two-layer scoring scheme	945:970	a two-layer scoring scheme for calculating a normalized matching score	945:1014	Specifically, a two-layer scoring scheme for calculating a normalized matching score is applied in the search procedure which enables the unbiased ranking of the matched glycans.
29580737	3	51	theme	quality	593:599	arg1	strategy					609:616	a reasonable quality control strategy	580:616	a reasonable quality control strategy for the statistical validation of reported results	580:667	Although extensive efforts have been made to the development of computational approaches for the automated interpretation of glycopeptide spectra, the previously appeared methods lack a reasonable quality control strategy for the statistical validation of reported results.
29580737	6	52	theme	proposed	1148:1155	arg1	method					1157:1162	our proposed method	1144:1162	our proposed method	1144:1162	Experimental analysis showed that our proposed method can report more structures with high confidence compared with previous approaches.
29580737	5	53	theme	normalized	990:999	arg1	score					1010:1014	a normalized matching score	988:1014	a normalized matching score	988:1014	Specifically, a two-layer scoring scheme for calculating a normalized matching score is applied in the search procedure which enables the unbiased ranking of the matched glycans.
29580737	1	54	theme	essential	136:144	arg1	step					146:149	an essential step	133:149	an essential step for the thorough investigation of the structure and function of protein	133:221	Glycan structure determination serves as an essential step for the thorough investigation of the structure and function of protein.
29580737	1	54	theme	essential	136:144	arg1	determination					109:121	Glycan structure determination	92:121	Glycan structure determination	92:121	Glycan structure determination serves as an essential step for the thorough investigation of the structure and function of protein.
29580737	0	55	dep	approach	3:10	arg1	strategy					82:89	target-decoy strategy	69:89	target-decoy strategy	69:89	An approach for N-linked glycan identification from MS/MS spectra by target-decoy strategy.
29580737	2	56	theme	glycopeptides	381:393	arg1	characterization					349:364	the characterization	345:364	the characterization of glycans and glycopeptides	345:393	Currently, appropriate sample preparation followed by tandem mass spectrometry has emerged as the dominant technique for the characterization of glycans and glycopeptides.
29580737	2	57	theme	sample	247:252	arg1	preparation					254:264	appropriate sample preparation	235:264	appropriate sample preparation followed by tandem mass spectrometry	235:301	Currently, appropriate sample preparation followed by tandem mass spectrometry has emerged as the dominant technique for the characterization of glycans and glycopeptides.
29580737	3	58	theme	appeared	558:565	arg1	methods					567:573	the previously appeared methods	543:573	the previously appeared methods	543:573	Although extensive efforts have been made to the development of computational approaches for the automated interpretation of glycopeptide spectra, the previously appeared methods lack a reasonable quality control strategy for the statistical validation of reported results.
30926029	7	0	theme	crystalline	1830:1840	arg1	lens					1842:1845	human crystalline lens	1824:1845	human crystalline lens	1824:1845	The design of mMIL-125@Au@L-Cys not only served as a multifunctional probe for efficient identification of N-linked glycopeptides and phosphopeptides in human crystalline lens, but also set a precedent for fabricating more MOFs with post-modifications for further proteomics research.
30926029	7	1	from	phosphopeptides	1805:1819	arg1	lens					1842:1845	human crystalline lens	1824:1845	human crystalline lens	1824:1845	The design of mMIL-125@Au@L-Cys not only served as a multifunctional probe for efficient identification of N-linked glycopeptides and phosphopeptides in human crystalline lens, but also set a precedent for fabricating more MOFs with post-modifications for further proteomics research.
30926029	5	2	gly	glycoproteins	1343:1355	arg1	glycoproteins					1343:1355	35 glycoproteins	1340:1355	35 glycoproteins	1340:1355	In the end, 81 N-linked glycopeptides corresponding to 35 glycoproteins and 175 phosphopeptides ascribed to 55 phosphorylated proteins were identified, respectively.
30926029	1	3	gly	glycopeptides	213:225	arg2	glycopeptides					213:225	N-linked glycopeptides	204:225	N-linked glycopeptides	204:225	Highly selective enrichment of N-linked glycopeptides and phosphopeptides from complex biological samples is extremely important prior to mass spectrometry analysis due to their low abundance as well as numerous extrinsic interferences.
30926029	1	4	theme	spectrometry	316:327	arg1	analysis					329:336	mass spectrometry analysis	311:336	mass spectrometry analysis	311:336	Highly selective enrichment of N-linked glycopeptides and phosphopeptides from complex biological samples is extremely important prior to mass spectrometry analysis due to their low abundance as well as numerous extrinsic interferences.
30926029	3	5	used	utilized	742:749	arg2	material					721:728	the as-prepared material	705:728	the as-prepared material	705:728	By combining hydrophilic interaction chromatography (HILIC) with metal oxide affinity chromatography (MOAC), the as-prepared material was firstly utilized to identify N-linked glycopeptides and phosphopeptides from tryptic digests of horseradish peroxidase (HRP) and beta-casein (β-casein), respectively, with the help of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and exhibited outstanding sensitivity (0.1 fmol μL-1), great reusability (5 circles) and high selectivity (1: 100).
30926029	6	6	theme	centers	1568:1574	arg1	sites					1595:1599	numerous reaction sites	1577:1599	numerous reaction sites on the large surface of MOFs	1577:1628	The remarkable results were benefitted from the merits of improved hydrophilicity from L-Cys, strong affinity of TiO centers, numerous reaction sites on the large surface of MOFs and superparamagnetism from Fe3O4 cores.
30926029	6	6	theme	centers	1568:1574	arg1	superparamagnetism					1634:1651	superparamagnetism	1634:1651	superparamagnetism	1634:1651	The remarkable results were benefitted from the merits of improved hydrophilicity from L-Cys, strong affinity of TiO centers, numerous reaction sites on the large surface of MOFs and superparamagnetism from Fe3O4 cores.
30926029	6	6	theme	centers	1568:1574	arg1	L-Cys					1538:1542	L-Cys	1538:1542	L-Cys	1538:1542	The remarkable results were benefitted from the merits of improved hydrophilicity from L-Cys, strong affinity of TiO centers, numerous reaction sites on the large surface of MOFs and superparamagnetism from Fe3O4 cores.
30926029	6	6	theme	centers	1568:1574	arg1	affinity					1552:1559	strong affinity	1545:1559	strong affinity of TiO centers	1545:1574	The remarkable results were benefitted from the merits of improved hydrophilicity from L-Cys, strong affinity of TiO centers, numerous reaction sites on the large surface of MOFs and superparamagnetism from Fe3O4 cores.
30926029	6	6	theme	centers	1568:1574	arg1	centers					1568:1574	TiO centers	1564:1574	TiO centers	1564:1574	The remarkable results were benefitted from the merits of improved hydrophilicity from L-Cys, strong affinity of TiO centers, numerous reaction sites on the large surface of MOFs and superparamagnetism from Fe3O4 cores.
30926029	5	7	gly	glycopeptides	1309:1321	arg2	glycopeptides					1309:1321	81 N-linked glycopeptides	1297:1321	81 N-linked glycopeptides corresponding to 35 glycoproteins and 175 phosphopeptides ascribed to 55 phosphorylated proteins	1297:1418	In the end, 81 N-linked glycopeptides corresponding to 35 glycoproteins and 175 phosphopeptides ascribed to 55 phosphorylated proteins were identified, respectively.
30926029	6	8	theme	remarkable	1455:1464	arg1	results					1466:1472	The remarkable results	1451:1472	The remarkable results	1451:1472	The remarkable results were benefitted from the merits of improved hydrophilicity from L-Cys, strong affinity of TiO centers, numerous reaction sites on the large surface of MOFs and superparamagnetism from Fe3O4 cores.
30926029	7	9	theme	N-linked	1778:1785	arg1	glycopeptides					1787:1799	N-linked glycopeptides	1778:1799	N-linked glycopeptides	1778:1799	The design of mMIL-125@Au@L-Cys not only served as a multifunctional probe for efficient identification of N-linked glycopeptides and phosphopeptides in human crystalline lens, but also set a precedent for fabricating more MOFs with post-modifications for further proteomics research.
30926029	3	10	theme	N-linked	763:770	arg1	glycopeptides					772:784	N-linked glycopeptides	763:784	N-linked glycopeptides	763:784	By combining hydrophilic interaction chromatography (HILIC) with metal oxide affinity chromatography (MOAC), the as-prepared material was firstly utilized to identify N-linked glycopeptides and phosphopeptides from tryptic digests of horseradish peroxidase (HRP) and beta-casein (β-casein), respectively, with the help of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and exhibited outstanding sensitivity (0.1 fmol μL-1), great reusability (5 circles) and high selectivity (1: 100).
30926029	7	11	theme	proteomics	1935:1944	arg1	research					1946:1953	further proteomics research	1927:1953	further proteomics research	1927:1953	The design of mMIL-125@Au@L-Cys not only served as a multifunctional probe for efficient identification of N-linked glycopeptides and phosphopeptides in human crystalline lens, but also set a precedent for fabricating more MOFs with post-modifications for further proteomics research.
30926029	6	12	theme	strong	1545:1550	arg1	affinity					1552:1559	strong affinity	1545:1559	strong affinity of TiO centers	1545:1574	The remarkable results were benefitted from the merits of improved hydrophilicity from L-Cys, strong affinity of TiO centers, numerous reaction sites on the large surface of MOFs and superparamagnetism from Fe3O4 cores.
30926029	6	13	from	cores	1664:1668	arg1	merits					1499:1504	the merits	1495:1504	the merits of improved hydrophilicity from L-Cys, strong affinity of TiO centers, numerous reaction sites on the large surface of MOFs and superparamagnetism from Fe3O4 cores	1495:1668	The remarkable results were benefitted from the merits of improved hydrophilicity from L-Cys, strong affinity of TiO centers, numerous reaction sites on the large surface of MOFs and superparamagnetism from Fe3O4 cores.
30926029	0	14	theme	crystalline	155:165	arg1	lens					167:170	human crystalline lens	149:170	human crystalline lens	149:170	l-cysteine-modified metal-organic frameworks as multifunctional probes for efficient identification of N-linked glycopeptides and phosphopeptides in human crystalline lens.
30926029	1	15	theme	biological	260:269	arg1	samples					271:277	complex biological samples	252:277	complex biological samples	252:277	Highly selective enrichment of N-linked glycopeptides and phosphopeptides from complex biological samples is extremely important prior to mass spectrometry analysis due to their low abundance as well as numerous extrinsic interferences.
30926029	4	16	gly	glycopeptides	1187:1199	arg2	glycopeptides					1187:1199	glycopeptides	1187:1199	glycopeptides	1187:1199	Based on this, it was further applied into the enrichment of glycopeptides and phosphopeptides from tryptic digests of 100 μg human crystalline lens proteins.
30926029	3	17	theme	hydrophilic	609:619	arg1	chromatography					633:646	hydrophilic interaction chromatography	609:646	hydrophilic interaction chromatography (HILIC)	609:654	By combining hydrophilic interaction chromatography (HILIC) with metal oxide affinity chromatography (MOAC), the as-prepared material was firstly utilized to identify N-linked glycopeptides and phosphopeptides from tryptic digests of horseradish peroxidase (HRP) and beta-casein (β-casein), respectively, with the help of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and exhibited outstanding sensitivity (0.1 fmol μL-1), great reusability (5 circles) and high selectivity (1: 100).
30926029	3	17	theme	hydrophilic	609:619	arg1	HILIC					649:653	HILIC	649:653	HILIC	649:653	By combining hydrophilic interaction chromatography (HILIC) with metal oxide affinity chromatography (MOAC), the as-prepared material was firstly utilized to identify N-linked glycopeptides and phosphopeptides from tryptic digests of horseradish peroxidase (HRP) and beta-casein (β-casein), respectively, with the help of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and exhibited outstanding sensitivity (0.1 fmol μL-1), great reusability (5 circles) and high selectivity (1: 100).
30926029	1	18	from	samples	271:277	arg1	enrichment					190:199	Highly selective enrichment	173:199	Highly selective enrichment of N-linked glycopeptides and phosphopeptides from complex biological samples	173:277	Highly selective enrichment of N-linked glycopeptides and phosphopeptides from complex biological samples is extremely important prior to mass spectrometry analysis due to their low abundance as well as numerous extrinsic interferences.
30926029	0	19	from	identification	85:98	arg1	lens					167:170	human crystalline lens	149:170	human crystalline lens	149:170	l-cysteine-modified metal-organic frameworks as multifunctional probes for efficient identification of N-linked glycopeptides and phosphopeptides in human crystalline lens.
30926029	3	20	theme	horseradish	830:840	arg1	HRP					854:856	HRP	854:856	HRP	854:856	By combining hydrophilic interaction chromatography (HILIC) with metal oxide affinity chromatography (MOAC), the as-prepared material was firstly utilized to identify N-linked glycopeptides and phosphopeptides from tryptic digests of horseradish peroxidase (HRP) and beta-casein (β-casein), respectively, with the help of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and exhibited outstanding sensitivity (0.1 fmol μL-1), great reusability (5 circles) and high selectivity (1: 100).
30926029	3	20	theme	horseradish	830:840	arg1	peroxidase					842:851	horseradish peroxidase	830:851	horseradish peroxidase (HRP)	830:857	By combining hydrophilic interaction chromatography (HILIC) with metal oxide affinity chromatography (MOAC), the as-prepared material was firstly utilized to identify N-linked glycopeptides and phosphopeptides from tryptic digests of horseradish peroxidase (HRP) and beta-casein (β-casein), respectively, with the help of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and exhibited outstanding sensitivity (0.1 fmol μL-1), great reusability (5 circles) and high selectivity (1: 100).
30926029	7	21	theme	mMIL-125	1685:1692	arg1	L-Cys					1697:1701	mMIL-125@Au@L-Cys	1685:1701	mMIL-125@Au@L-Cys	1685:1701	The design of mMIL-125@Au@L-Cys not only served as a multifunctional probe for efficient identification of N-linked glycopeptides and phosphopeptides in human crystalline lens, but also set a precedent for fabricating more MOFs with post-modifications for further proteomics research.
30926029	3	22	theme	beta-casein	863:873	arg1	digests					819:825	tryptic digests	811:825	tryptic digests of horseradish peroxidase (HRP) and beta-casein (β-casein), respectively	811:898	By combining hydrophilic interaction chromatography (HILIC) with metal oxide affinity chromatography (MOAC), the as-prepared material was firstly utilized to identify N-linked glycopeptides and phosphopeptides from tryptic digests of horseradish peroxidase (HRP) and beta-casein (β-casein), respectively, with the help of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and exhibited outstanding sensitivity (0.1 fmol μL-1), great reusability (5 circles) and high selectivity (1: 100).
30926029	1	23	theme	extrinsic	385:393	arg1	interferences					395:407	numerous extrinsic interferences	376:407	their low abundance as well as numerous extrinsic interferences	345:407	Highly selective enrichment of N-linked glycopeptides and phosphopeptides from complex biological samples is extremely important prior to mass spectrometry analysis due to their low abundance as well as numerous extrinsic interferences.
30926029	6	24	theme	numerous	1577:1584	arg1	sites					1595:1599	numerous reaction sites	1577:1599	numerous reaction sites on the large surface of MOFs	1577:1628	The remarkable results were benefitted from the merits of improved hydrophilicity from L-Cys, strong affinity of TiO centers, numerous reaction sites on the large surface of MOFs and superparamagnetism from Fe3O4 cores.
30926029	6	24	theme	numerous	1577:1584	arg1	centers					1568:1574	TiO centers	1564:1574	TiO centers	1564:1574	The remarkable results were benefitted from the merits of improved hydrophilicity from L-Cys, strong affinity of TiO centers, numerous reaction sites on the large surface of MOFs and superparamagnetism from Fe3O4 cores.
30926029	0	25	theme	N-linked	103:110	arg1	glycopeptides					112:124	N-linked glycopeptides	103:124	N-linked glycopeptides	103:124	l-cysteine-modified metal-organic frameworks as multifunctional probes for efficient identification of N-linked glycopeptides and phosphopeptides in human crystalline lens.
30926029	1	26	theme	N-linked	204:211	arg1	glycopeptides					213:225	N-linked glycopeptides	204:225	N-linked glycopeptides	204:225	Highly selective enrichment of N-linked glycopeptides and phosphopeptides from complex biological samples is extremely important prior to mass spectrometry analysis due to their low abundance as well as numerous extrinsic interferences.
30926029	7	27	theme	glycopeptides	1787:1799	arg1	identification					1760:1773	efficient identification	1750:1773	efficient identification of N-linked glycopeptides and phosphopeptides in human crystalline lens	1750:1845	The design of mMIL-125@Au@L-Cys not only served as a multifunctional probe for efficient identification of N-linked glycopeptides and phosphopeptides in human crystalline lens, but also set a precedent for fabricating more MOFs with post-modifications for further proteomics research.
30926029	6	28	theme	Fe3O4	1658:1662	arg1	cores					1664:1668	Fe3O4 cores	1658:1668	Fe3O4 cores	1658:1668	The remarkable results were benefitted from the merits of improved hydrophilicity from L-Cys, strong affinity of TiO centers, numerous reaction sites on the large surface of MOFs and superparamagnetism from Fe3O4 cores.
30926029	4	29	from	digests	1234:1240	arg1	enrichment					1173:1182	the enrichment	1169:1182	the enrichment of glycopeptides and phosphopeptides from tryptic digests of 100 μg human crystalline lens proteins	1169:1282	Based on this, it was further applied into the enrichment of glycopeptides and phosphopeptides from tryptic digests of 100 μg human crystalline lens proteins.
30926029	7	30	theme	@	1696:1696	arg1	L-Cys					1697:1701	mMIL-125@Au@L-Cys	1685:1701	mMIL-125@Au@L-Cys	1685:1701	The design of mMIL-125@Au@L-Cys not only served as a multifunctional probe for efficient identification of N-linked glycopeptides and phosphopeptides in human crystalline lens, but also set a precedent for fabricating more MOFs with post-modifications for further proteomics research.
30926029	7	31	gly	glycopeptides	1787:1799	arg2	glycopeptides					1787:1799	N-linked glycopeptides	1778:1799	N-linked glycopeptides	1778:1799	The design of mMIL-125@Au@L-Cys not only served as a multifunctional probe for efficient identification of N-linked glycopeptides and phosphopeptides in human crystalline lens, but also set a precedent for fabricating more MOFs with post-modifications for further proteomics research.
30926029	2	32	theme	Au	541:542	arg1	L-Cys					525:529	Fe3O4@PDA@MIL-125@Au@L-Cys	504:529	Fe3O4@PDA@MIL-125@Au@L-Cys (mMIL-125@Au@L-Cys)	504:549	In this work, l-cysteine (L-Cys)-modified multifunctional metal-organic frameworks denoted as Fe3O4@PDA@MIL-125@Au@L-Cys (mMIL-125@Au@L-Cys) were prepared by modifications step by step.
30926029	2	32	theme	Au	541:542	arg1	L-Cys					544:548	mMIL-125@Au@L-Cys	532:548	mMIL-125@Au@L-Cys	532:548	In this work, l-cysteine (L-Cys)-modified multifunctional metal-organic frameworks denoted as Fe3O4@PDA@MIL-125@Au@L-Cys (mMIL-125@Au@L-Cys) were prepared by modifications step by step.
30926029	2	33	theme	-modified	442:450	arg1	frameworks					482:491	l-cysteine (L-Cys)-modified multifunctional metal-organic frameworks	424:491	l-cysteine (L-Cys)-modified multifunctional metal-organic frameworks denoted as Fe3O4@PDA@MIL-125@Au@L-Cys (mMIL-125@Au@L-Cys)	424:549	In this work, l-cysteine (L-Cys)-modified multifunctional metal-organic frameworks denoted as Fe3O4@PDA@MIL-125@Au@L-Cys (mMIL-125@Au@L-Cys) were prepared by modifications step by step.
30926029	0	34	gly	glycopeptides	112:124	arg2	glycopeptides					112:124	N-linked glycopeptides	103:124	N-linked glycopeptides	103:124	l-cysteine-modified metal-organic frameworks as multifunctional probes for efficient identification of N-linked glycopeptides and phosphopeptides in human crystalline lens.
30926029	7	35	theme	@	1693:1693	arg1	L-Cys					1697:1701	mMIL-125@Au@L-Cys	1685:1701	mMIL-125@Au@L-Cys	1685:1701	The design of mMIL-125@Au@L-Cys not only served as a multifunctional probe for efficient identification of N-linked glycopeptides and phosphopeptides in human crystalline lens, but also set a precedent for fabricating more MOFs with post-modifications for further proteomics research.
30926029	2	36	theme	PDA	510:512	arg1	L-Cys					525:529	Fe3O4@PDA@MIL-125@Au@L-Cys	504:529	Fe3O4@PDA@MIL-125@Au@L-Cys (mMIL-125@Au@L-Cys)	504:549	In this work, l-cysteine (L-Cys)-modified multifunctional metal-organic frameworks denoted as Fe3O4@PDA@MIL-125@Au@L-Cys (mMIL-125@Au@L-Cys) were prepared by modifications step by step.
30926029	2	36	theme	PDA	510:512	arg1	L-Cys					544:548	mMIL-125@Au@L-Cys	532:548	mMIL-125@Au@L-Cys	532:548	In this work, l-cysteine (L-Cys)-modified multifunctional metal-organic frameworks denoted as Fe3O4@PDA@MIL-125@Au@L-Cys (mMIL-125@Au@L-Cys) were prepared by modifications step by step.
30926029	0	37	theme	l-cysteine-modified	0:18	arg1	frameworks					34:43	l-cysteine-modified metal-organic frameworks	0:43	l-cysteine-modified metal-organic frameworks as multifunctional probes for efficient identification of N-linked glycopeptides and phosphopeptides in human crystalline lens.	0:171	l-cysteine-modified metal-organic frameworks as multifunctional probes for efficient identification of N-linked glycopeptides and phosphopeptides in human crystalline lens.
30926029	3	38	theme	high	1099:1102	arg1	100					1120:1122	100	1120:1122	100	1120:1122	By combining hydrophilic interaction chromatography (HILIC) with metal oxide affinity chromatography (MOAC), the as-prepared material was firstly utilized to identify N-linked glycopeptides and phosphopeptides from tryptic digests of horseradish peroxidase (HRP) and beta-casein (β-casein), respectively, with the help of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and exhibited outstanding sensitivity (0.1 fmol μL-1), great reusability (5 circles) and high selectivity (1: 100).
30926029	3	38	theme	high	1099:1102	arg1	selectivity					1104:1114	high selectivity	1099:1114	high selectivity (1: 100)	1099:1123	By combining hydrophilic interaction chromatography (HILIC) with metal oxide affinity chromatography (MOAC), the as-prepared material was firstly utilized to identify N-linked glycopeptides and phosphopeptides from tryptic digests of horseradish peroxidase (HRP) and beta-casein (β-casein), respectively, with the help of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and exhibited outstanding sensitivity (0.1 fmol μL-1), great reusability (5 circles) and high selectivity (1: 100).
30926029	6	39	theme	MOFs	1625:1628	arg1	surface					1614:1620	the large surface	1604:1620	the large surface of MOFs	1604:1628	The remarkable results were benefitted from the merits of improved hydrophilicity from L-Cys, strong affinity of TiO centers, numerous reaction sites on the large surface of MOFs and superparamagnetism from Fe3O4 cores.
30926029	3	40	theme	ionization	951:960	arg1	spectrometry					982:993	matrix-assisted laser desorption ionization time-of-flight mass spectrometry	918:993	matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS)	918:1008	By combining hydrophilic interaction chromatography (HILIC) with metal oxide affinity chromatography (MOAC), the as-prepared material was firstly utilized to identify N-linked glycopeptides and phosphopeptides from tryptic digests of horseradish peroxidase (HRP) and beta-casein (β-casein), respectively, with the help of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and exhibited outstanding sensitivity (0.1 fmol μL-1), great reusability (5 circles) and high selectivity (1: 100).
30926029	3	40	theme	ionization	951:960	arg1	MS					1006:1007	MALDI-TOF MS	996:1007	MALDI-TOF MS	996:1007	By combining hydrophilic interaction chromatography (HILIC) with metal oxide affinity chromatography (MOAC), the as-prepared material was firstly utilized to identify N-linked glycopeptides and phosphopeptides from tryptic digests of horseradish peroxidase (HRP) and beta-casein (β-casein), respectively, with the help of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and exhibited outstanding sensitivity (0.1 fmol μL-1), great reusability (5 circles) and high selectivity (1: 100).
30926029	3	41	theme	oxide	667:671	arg1	chromatography					682:695	metal oxide affinity chromatography	661:695	metal oxide affinity chromatography (MOAC)	661:702	By combining hydrophilic interaction chromatography (HILIC) with metal oxide affinity chromatography (MOAC), the as-prepared material was firstly utilized to identify N-linked glycopeptides and phosphopeptides from tryptic digests of horseradish peroxidase (HRP) and beta-casein (β-casein), respectively, with the help of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and exhibited outstanding sensitivity (0.1 fmol μL-1), great reusability (5 circles) and high selectivity (1: 100).
30926029	3	41	theme	oxide	667:671	arg1	MOAC					698:701	MOAC	698:701	MOAC	698:701	By combining hydrophilic interaction chromatography (HILIC) with metal oxide affinity chromatography (MOAC), the as-prepared material was firstly utilized to identify N-linked glycopeptides and phosphopeptides from tryptic digests of horseradish peroxidase (HRP) and beta-casein (β-casein), respectively, with the help of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and exhibited outstanding sensitivity (0.1 fmol μL-1), great reusability (5 circles) and high selectivity (1: 100).
30926029	2	42	theme	Fe3O4	504:508	arg1	L-Cys					525:529	Fe3O4@PDA@MIL-125@Au@L-Cys	504:529	Fe3O4@PDA@MIL-125@Au@L-Cys (mMIL-125@Au@L-Cys)	504:549	In this work, l-cysteine (L-Cys)-modified multifunctional metal-organic frameworks denoted as Fe3O4@PDA@MIL-125@Au@L-Cys (mMIL-125@Au@L-Cys) were prepared by modifications step by step.
30926029	2	42	theme	Fe3O4	504:508	arg1	L-Cys					544:548	mMIL-125@Au@L-Cys	532:548	mMIL-125@Au@L-Cys	532:548	In this work, l-cysteine (L-Cys)-modified multifunctional metal-organic frameworks denoted as Fe3O4@PDA@MIL-125@Au@L-Cys (mMIL-125@Au@L-Cys) were prepared by modifications step by step.
30926029	1	43	theme	low	351:353	arg1	abundance					355:363	their low abundance	345:363	their low abundance as well as numerous extrinsic interferences	345:407	Highly selective enrichment of N-linked glycopeptides and phosphopeptides from complex biological samples is extremely important prior to mass spectrometry analysis due to their low abundance as well as numerous extrinsic interferences.
30926029	3	44	theme	laser	934:938	arg1	spectrometry					982:993	matrix-assisted laser desorption ionization time-of-flight mass spectrometry	918:993	matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS)	918:1008	By combining hydrophilic interaction chromatography (HILIC) with metal oxide affinity chromatography (MOAC), the as-prepared material was firstly utilized to identify N-linked glycopeptides and phosphopeptides from tryptic digests of horseradish peroxidase (HRP) and beta-casein (β-casein), respectively, with the help of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and exhibited outstanding sensitivity (0.1 fmol μL-1), great reusability (5 circles) and high selectivity (1: 100).
30926029	3	44	theme	laser	934:938	arg1	MS					1006:1007	MALDI-TOF MS	996:1007	MALDI-TOF MS	996:1007	By combining hydrophilic interaction chromatography (HILIC) with metal oxide affinity chromatography (MOAC), the as-prepared material was firstly utilized to identify N-linked glycopeptides and phosphopeptides from tryptic digests of horseradish peroxidase (HRP) and beta-casein (β-casein), respectively, with the help of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and exhibited outstanding sensitivity (0.1 fmol μL-1), great reusability (5 circles) and high selectivity (1: 100).
30926029	2	45	theme	Au	522:523	arg1	L-Cys					525:529	Fe3O4@PDA@MIL-125@Au@L-Cys	504:529	Fe3O4@PDA@MIL-125@Au@L-Cys (mMIL-125@Au@L-Cys)	504:549	In this work, l-cysteine (L-Cys)-modified multifunctional metal-organic frameworks denoted as Fe3O4@PDA@MIL-125@Au@L-Cys (mMIL-125@Au@L-Cys) were prepared by modifications step by step.
30926029	2	45	theme	Au	522:523	arg1	L-Cys					544:548	mMIL-125@Au@L-Cys	532:548	mMIL-125@Au@L-Cys	532:548	In this work, l-cysteine (L-Cys)-modified multifunctional metal-organic frameworks denoted as Fe3O4@PDA@MIL-125@Au@L-Cys (mMIL-125@Au@L-Cys) were prepared by modifications step by step.
30926029	4	46	theme	proteins	1275:1282	arg1	digests					1234:1240	tryptic digests	1226:1240	tryptic digests of 100 μg human crystalline lens proteins	1226:1282	Based on this, it was further applied into the enrichment of glycopeptides and phosphopeptides from tryptic digests of 100 μg human crystalline lens proteins.
30926029	2	47	theme	metal-organic	468:480	arg1	frameworks					482:491	l-cysteine (L-Cys)-modified multifunctional metal-organic frameworks	424:491	l-cysteine (L-Cys)-modified multifunctional metal-organic frameworks denoted as Fe3O4@PDA@MIL-125@Au@L-Cys (mMIL-125@Au@L-Cys)	424:549	In this work, l-cysteine (L-Cys)-modified multifunctional metal-organic frameworks denoted as Fe3O4@PDA@MIL-125@Au@L-Cys (mMIL-125@Au@L-Cys) were prepared by modifications step by step.
30926029	0	48	theme	multifunctional	48:62	arg1	probes					64:69	multifunctional probes	48:69	multifunctional probes for efficient identification of N-linked glycopeptides and phosphopeptides in human crystalline lens	48:170	l-cysteine-modified metal-organic frameworks as multifunctional probes for efficient identification of N-linked glycopeptides and phosphopeptides in human crystalline lens.
30926029	6	49	from	merits	1499:1504	arg1	superparamagnetism					1634:1651	superparamagnetism	1634:1651	superparamagnetism	1634:1651	The remarkable results were benefitted from the merits of improved hydrophilicity from L-Cys, strong affinity of TiO centers, numerous reaction sites on the large surface of MOFs and superparamagnetism from Fe3O4 cores.
30926029	6	49	from	merits	1499:1504	arg1	sites					1595:1599	numerous reaction sites	1577:1599	numerous reaction sites on the large surface of MOFs	1577:1628	The remarkable results were benefitted from the merits of improved hydrophilicity from L-Cys, strong affinity of TiO centers, numerous reaction sites on the large surface of MOFs and superparamagnetism from Fe3O4 cores.
30926029	6	49	from	merits	1499:1504	arg1	L-Cys					1538:1542	L-Cys	1538:1542	L-Cys	1538:1542	The remarkable results were benefitted from the merits of improved hydrophilicity from L-Cys, strong affinity of TiO centers, numerous reaction sites on the large surface of MOFs and superparamagnetism from Fe3O4 cores.
30926029	6	49	from	merits	1499:1504	arg1	affinity					1552:1559	strong affinity	1545:1559	strong affinity of TiO centers	1545:1574	The remarkable results were benefitted from the merits of improved hydrophilicity from L-Cys, strong affinity of TiO centers, numerous reaction sites on the large surface of MOFs and superparamagnetism from Fe3O4 cores.
30926029	6	49	from	merits	1499:1504	arg1	centers					1568:1574	TiO centers	1564:1574	TiO centers	1564:1574	The remarkable results were benefitted from the merits of improved hydrophilicity from L-Cys, strong affinity of TiO centers, numerous reaction sites on the large surface of MOFs and superparamagnetism from Fe3O4 cores.
30926029	3	50	from	digests	819:825	arg1	phosphopeptides					790:804	phosphopeptides	790:804	phosphopeptides	790:804	By combining hydrophilic interaction chromatography (HILIC) with metal oxide affinity chromatography (MOAC), the as-prepared material was firstly utilized to identify N-linked glycopeptides and phosphopeptides from tryptic digests of horseradish peroxidase (HRP) and beta-casein (β-casein), respectively, with the help of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and exhibited outstanding sensitivity (0.1 fmol μL-1), great reusability (5 circles) and high selectivity (1: 100).
30926029	3	50	from	digests	819:825	arg1	glycopeptides					772:784	N-linked glycopeptides	763:784	N-linked glycopeptides	763:784	By combining hydrophilic interaction chromatography (HILIC) with metal oxide affinity chromatography (MOAC), the as-prepared material was firstly utilized to identify N-linked glycopeptides and phosphopeptides from tryptic digests of horseradish peroxidase (HRP) and beta-casein (β-casein), respectively, with the help of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and exhibited outstanding sensitivity (0.1 fmol μL-1), great reusability (5 circles) and high selectivity (1: 100).
30926029	7	51	theme	more	1889:1892	arg1	MOFs					1894:1897	more MOFs	1889:1897	more MOFs	1889:1897	The design of mMIL-125@Au@L-Cys not only served as a multifunctional probe for efficient identification of N-linked glycopeptides and phosphopeptides in human crystalline lens, but also set a precedent for fabricating more MOFs with post-modifications for further proteomics research.
30926029	3	52	theme	mass	977:980	arg1	spectrometry					982:993	matrix-assisted laser desorption ionization time-of-flight mass spectrometry	918:993	matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS)	918:1008	By combining hydrophilic interaction chromatography (HILIC) with metal oxide affinity chromatography (MOAC), the as-prepared material was firstly utilized to identify N-linked glycopeptides and phosphopeptides from tryptic digests of horseradish peroxidase (HRP) and beta-casein (β-casein), respectively, with the help of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and exhibited outstanding sensitivity (0.1 fmol μL-1), great reusability (5 circles) and high selectivity (1: 100).
30926029	3	52	theme	mass	977:980	arg1	MS					1006:1007	MALDI-TOF MS	996:1007	MALDI-TOF MS	996:1007	By combining hydrophilic interaction chromatography (HILIC) with metal oxide affinity chromatography (MOAC), the as-prepared material was firstly utilized to identify N-linked glycopeptides and phosphopeptides from tryptic digests of horseradish peroxidase (HRP) and beta-casein (β-casein), respectively, with the help of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and exhibited outstanding sensitivity (0.1 fmol μL-1), great reusability (5 circles) and high selectivity (1: 100).
30926029	7	53	from	identification	1760:1773	arg1	lens					1842:1845	human crystalline lens	1824:1845	human crystalline lens	1824:1845	The design of mMIL-125@Au@L-Cys not only served as a multifunctional probe for efficient identification of N-linked glycopeptides and phosphopeptides in human crystalline lens, but also set a precedent for fabricating more MOFs with post-modifications for further proteomics research.
30926029	4	54	theme	100 μg	1245:1250	arg1	proteins					1275:1282	100 μg human crystalline lens proteins	1245:1282	100 μg human crystalline lens proteins	1245:1282	Based on this, it was further applied into the enrichment of glycopeptides and phosphopeptides from tryptic digests of 100 μg human crystalline lens proteins.
30926029	6	55	theme	improved	1509:1516	arg1	hydrophilicity					1518:1531	improved hydrophilicity	1509:1531	improved hydrophilicity from L-Cys, strong affinity of TiO centers, numerous reaction sites on the large surface of MOFs and superparamagnetism	1509:1651	The remarkable results were benefitted from the merits of improved hydrophilicity from L-Cys, strong affinity of TiO centers, numerous reaction sites on the large surface of MOFs and superparamagnetism from Fe3O4 cores.
30926029	4	56	theme	crystalline	1258:1268	arg1	proteins					1275:1282	100 μg human crystalline lens proteins	1245:1282	100 μg human crystalline lens proteins	1245:1282	Based on this, it was further applied into the enrichment of glycopeptides and phosphopeptides from tryptic digests of 100 μg human crystalline lens proteins.
30926029	7	57	theme	human	1824:1828	arg1	lens					1842:1845	human crystalline lens	1824:1845	human crystalline lens	1824:1845	The design of mMIL-125@Au@L-Cys not only served as a multifunctional probe for efficient identification of N-linked glycopeptides and phosphopeptides in human crystalline lens, but also set a precedent for fabricating more MOFs with post-modifications for further proteomics research.
30926029	3	58	theme	as-prepared	709:719	arg1	material					721:728	the as-prepared material	705:728	the as-prepared material	705:728	By combining hydrophilic interaction chromatography (HILIC) with metal oxide affinity chromatography (MOAC), the as-prepared material was firstly utilized to identify N-linked glycopeptides and phosphopeptides from tryptic digests of horseradish peroxidase (HRP) and beta-casein (β-casein), respectively, with the help of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and exhibited outstanding sensitivity (0.1 fmol μL-1), great reusability (5 circles) and high selectivity (1: 100).
30926029	1	59	theme	mass	311:314	arg1	spectrometry					316:327	mass spectrometry	311:327	mass spectrometry analysis	311:336	Highly selective enrichment of N-linked glycopeptides and phosphopeptides from complex biological samples is extremely important prior to mass spectrometry analysis due to their low abundance as well as numerous extrinsic interferences.
30926029	7	60	theme	phosphopeptides	1805:1819	arg1	identification					1760:1773	efficient identification	1750:1773	efficient identification of N-linked glycopeptides and phosphopeptides in human crystalline lens	1750:1845	The design of mMIL-125@Au@L-Cys not only served as a multifunctional probe for efficient identification of N-linked glycopeptides and phosphopeptides in human crystalline lens, but also set a precedent for fabricating more MOFs with post-modifications for further proteomics research.
30926029	0	61	link	N-linked	103:110	arg1	glycopeptides					112:124	N-linked glycopeptides	103:124	N-linked glycopeptides	103:124	l-cysteine-modified metal-organic frameworks as multifunctional probes for efficient identification of N-linked glycopeptides and phosphopeptides in human crystalline lens.
30926029	3	62	theme	outstanding	1024:1034	arg1	0.1 fmol μL-1					1049:1061	0.1 fmol μL-1	1049:1061	0.1 fmol μL-1	1049:1061	By combining hydrophilic interaction chromatography (HILIC) with metal oxide affinity chromatography (MOAC), the as-prepared material was firstly utilized to identify N-linked glycopeptides and phosphopeptides from tryptic digests of horseradish peroxidase (HRP) and beta-casein (β-casein), respectively, with the help of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and exhibited outstanding sensitivity (0.1 fmol μL-1), great reusability (5 circles) and high selectivity (1: 100).
30926029	3	62	theme	outstanding	1024:1034	arg1	sensitivity					1036:1046	outstanding sensitivity	1024:1046	outstanding sensitivity (0.1 fmol μL-1)	1024:1062	By combining hydrophilic interaction chromatography (HILIC) with metal oxide affinity chromatography (MOAC), the as-prepared material was firstly utilized to identify N-linked glycopeptides and phosphopeptides from tryptic digests of horseradish peroxidase (HRP) and beta-casein (β-casein), respectively, with the help of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and exhibited outstanding sensitivity (0.1 fmol μL-1), great reusability (5 circles) and high selectivity (1: 100).
30926029	6	63	theme	TiO	1564:1566	arg1	centers					1568:1574	TiO centers	1564:1574	TiO centers	1564:1574	The remarkable results were benefitted from the merits of improved hydrophilicity from L-Cys, strong affinity of TiO centers, numerous reaction sites on the large surface of MOFs and superparamagnetism from Fe3O4 cores.
30926029	1	64	theme	glycopeptides	213:225	arg1	enrichment					190:199	Highly selective enrichment	173:199	Highly selective enrichment of N-linked glycopeptides and phosphopeptides from complex biological samples	173:277	Highly selective enrichment of N-linked glycopeptides and phosphopeptides from complex biological samples is extremely important prior to mass spectrometry analysis due to their low abundance as well as numerous extrinsic interferences.
30926029	3	65	theme	great	1065:1069	arg1	circles					1086:1092	5 circles	1084:1092	5 circles	1084:1092	By combining hydrophilic interaction chromatography (HILIC) with metal oxide affinity chromatography (MOAC), the as-prepared material was firstly utilized to identify N-linked glycopeptides and phosphopeptides from tryptic digests of horseradish peroxidase (HRP) and beta-casein (β-casein), respectively, with the help of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and exhibited outstanding sensitivity (0.1 fmol μL-1), great reusability (5 circles) and high selectivity (1: 100).
30926029	3	65	theme	great	1065:1069	arg1	reusability					1071:1081	great reusability	1065:1081	great reusability (5 circles)	1065:1093	By combining hydrophilic interaction chromatography (HILIC) with metal oxide affinity chromatography (MOAC), the as-prepared material was firstly utilized to identify N-linked glycopeptides and phosphopeptides from tryptic digests of horseradish peroxidase (HRP) and beta-casein (β-casein), respectively, with the help of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and exhibited outstanding sensitivity (0.1 fmol μL-1), great reusability (5 circles) and high selectivity (1: 100).
30926029	1	66	theme	phosphopeptides	231:245	arg1	enrichment					190:199	Highly selective enrichment	173:199	Highly selective enrichment of N-linked glycopeptides and phosphopeptides from complex biological samples	173:277	Highly selective enrichment of N-linked glycopeptides and phosphopeptides from complex biological samples is extremely important prior to mass spectrometry analysis due to their low abundance as well as numerous extrinsic interferences.
30926029	7	67	theme	efficient	1750:1758	arg1	identification					1760:1773	efficient identification	1750:1773	efficient identification of N-linked glycopeptides and phosphopeptides in human crystalline lens	1750:1845	The design of mMIL-125@Au@L-Cys not only served as a multifunctional probe for efficient identification of N-linked glycopeptides and phosphopeptides in human crystalline lens, but also set a precedent for fabricating more MOFs with post-modifications for further proteomics research.
30926029	1	68	theme	complex	252:258	arg1	samples					271:277	complex biological samples	252:277	complex biological samples	252:277	Highly selective enrichment of N-linked glycopeptides and phosphopeptides from complex biological samples is extremely important prior to mass spectrometry analysis due to their low abundance as well as numerous extrinsic interferences.
30926029	6	69	from	superparamagnetism	1634:1651	arg1	surface					1614:1620	the large surface	1604:1620	the large surface of MOFs	1604:1628	The remarkable results were benefitted from the merits of improved hydrophilicity from L-Cys, strong affinity of TiO centers, numerous reaction sites on the large surface of MOFs and superparamagnetism from Fe3O4 cores.
30926029	6	69	from	superparamagnetism	1634:1651	arg1	merits					1499:1504	the merits	1495:1504	the merits of improved hydrophilicity from L-Cys, strong affinity of TiO centers, numerous reaction sites on the large surface of MOFs and superparamagnetism from Fe3O4 cores	1495:1668	The remarkable results were benefitted from the merits of improved hydrophilicity from L-Cys, strong affinity of TiO centers, numerous reaction sites on the large surface of MOFs and superparamagnetism from Fe3O4 cores.
30926029	6	69	from	superparamagnetism	1634:1651	arg1	hydrophilicity					1518:1531	improved hydrophilicity	1509:1531	improved hydrophilicity from L-Cys, strong affinity of TiO centers, numerous reaction sites on the large surface of MOFs and superparamagnetism	1509:1651	The remarkable results were benefitted from the merits of improved hydrophilicity from L-Cys, strong affinity of TiO centers, numerous reaction sites on the large surface of MOFs and superparamagnetism from Fe3O4 cores.
30926029	3	70	theme	tryptic	811:817	arg1	digests					819:825	tryptic digests	811:825	tryptic digests of horseradish peroxidase (HRP) and beta-casein (β-casein), respectively	811:898	By combining hydrophilic interaction chromatography (HILIC) with metal oxide affinity chromatography (MOAC), the as-prepared material was firstly utilized to identify N-linked glycopeptides and phosphopeptides from tryptic digests of horseradish peroxidase (HRP) and beta-casein (β-casein), respectively, with the help of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and exhibited outstanding sensitivity (0.1 fmol μL-1), great reusability (5 circles) and high selectivity (1: 100).
30926029	6	71	theme	large	1608:1612	arg1	surface					1614:1620	the large surface	1604:1620	the large surface of MOFs	1604:1628	The remarkable results were benefitted from the merits of improved hydrophilicity from L-Cys, strong affinity of TiO centers, numerous reaction sites on the large surface of MOFs and superparamagnetism from Fe3O4 cores.
30926029	2	72	theme	modifications	568:580	arg1	step					582:585	modifications step	568:585	modifications step	568:585	In this work, l-cysteine (L-Cys)-modified multifunctional metal-organic frameworks denoted as Fe3O4@PDA@MIL-125@Au@L-Cys (mMIL-125@Au@L-Cys) were prepared by modifications step by step.
30926029	3	73	theme	peroxidase	842:851	arg1	digests					819:825	tryptic digests	811:825	tryptic digests of horseradish peroxidase (HRP) and beta-casein (β-casein), respectively	811:898	By combining hydrophilic interaction chromatography (HILIC) with metal oxide affinity chromatography (MOAC), the as-prepared material was firstly utilized to identify N-linked glycopeptides and phosphopeptides from tryptic digests of horseradish peroxidase (HRP) and beta-casein (β-casein), respectively, with the help of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and exhibited outstanding sensitivity (0.1 fmol μL-1), great reusability (5 circles) and high selectivity (1: 100).
30926029	3	74	theme	interaction	621:631	arg1	chromatography					633:646	hydrophilic interaction chromatography	609:646	hydrophilic interaction chromatography (HILIC)	609:654	By combining hydrophilic interaction chromatography (HILIC) with metal oxide affinity chromatography (MOAC), the as-prepared material was firstly utilized to identify N-linked glycopeptides and phosphopeptides from tryptic digests of horseradish peroxidase (HRP) and beta-casein (β-casein), respectively, with the help of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and exhibited outstanding sensitivity (0.1 fmol μL-1), great reusability (5 circles) and high selectivity (1: 100).
30926029	3	74	theme	interaction	621:631	arg1	HILIC					649:653	HILIC	649:653	HILIC	649:653	By combining hydrophilic interaction chromatography (HILIC) with metal oxide affinity chromatography (MOAC), the as-prepared material was firstly utilized to identify N-linked glycopeptides and phosphopeptides from tryptic digests of horseradish peroxidase (HRP) and beta-casein (β-casein), respectively, with the help of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and exhibited outstanding sensitivity (0.1 fmol μL-1), great reusability (5 circles) and high selectivity (1: 100).
30926029	6	75	theme	reaction	1586:1593	arg1	sites					1595:1599	numerous reaction sites	1577:1599	numerous reaction sites on the large surface of MOFs	1577:1628	The remarkable results were benefitted from the merits of improved hydrophilicity from L-Cys, strong affinity of TiO centers, numerous reaction sites on the large surface of MOFs and superparamagnetism from Fe3O4 cores.
30926029	6	75	theme	reaction	1586:1593	arg1	centers					1568:1574	TiO centers	1564:1574	TiO centers	1564:1574	The remarkable results were benefitted from the merits of improved hydrophilicity from L-Cys, strong affinity of TiO centers, numerous reaction sites on the large surface of MOFs and superparamagnetism from Fe3O4 cores.
30926029	1	76	theme	selective	180:188	arg1	enrichment					190:199	Highly selective enrichment	173:199	Highly selective enrichment of N-linked glycopeptides and phosphopeptides from complex biological samples	173:277	Highly selective enrichment of N-linked glycopeptides and phosphopeptides from complex biological samples is extremely important prior to mass spectrometry analysis due to their low abundance as well as numerous extrinsic interferences.
30926029	7	77	theme	multifunctional	1724:1738	arg1	precedent					1863:1871	a precedent	1861:1871	a precedent for fabricating more MOFs with post-modifications for further proteomics research	1861:1953	The design of mMIL-125@Au@L-Cys not only served as a multifunctional probe for efficient identification of N-linked glycopeptides and phosphopeptides in human crystalline lens, but also set a precedent for fabricating more MOFs with post-modifications for further proteomics research.
30926029	7	77	theme	multifunctional	1724:1738	arg1	probe					1740:1744	a multifunctional probe	1722:1744	a multifunctional probe for efficient identification of N-linked glycopeptides and phosphopeptides in human crystalline lens	1722:1845	The design of mMIL-125@Au@L-Cys not only served as a multifunctional probe for efficient identification of N-linked glycopeptides and phosphopeptides in human crystalline lens, but also set a precedent for fabricating more MOFs with post-modifications for further proteomics research.
30926029	7	77	theme	multifunctional	1724:1738	arg1	design					1675:1680	The design	1671:1680	The design of mMIL-125@Au@L-Cys	1671:1701	The design of mMIL-125@Au@L-Cys not only served as a multifunctional probe for efficient identification of N-linked glycopeptides and phosphopeptides in human crystalline lens, but also set a precedent for fabricating more MOFs with post-modifications for further proteomics research.
30926029	5	78	link	81 N-linked	1297:1307	arg1	glycopeptides					1309:1321	81 N-linked glycopeptides	1297:1321	81 N-linked glycopeptides corresponding to 35 glycoproteins and 175 phosphopeptides ascribed to 55 phosphorylated proteins	1297:1418	In the end, 81 N-linked glycopeptides corresponding to 35 glycoproteins and 175 phosphopeptides ascribed to 55 phosphorylated proteins were identified, respectively.
30926029	3	79	link	N-linked	763:770	arg1	glycopeptides					772:784	N-linked glycopeptides	763:784	N-linked glycopeptides	763:784	By combining hydrophilic interaction chromatography (HILIC) with metal oxide affinity chromatography (MOAC), the as-prepared material was firstly utilized to identify N-linked glycopeptides and phosphopeptides from tryptic digests of horseradish peroxidase (HRP) and beta-casein (β-casein), respectively, with the help of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and exhibited outstanding sensitivity (0.1 fmol μL-1), great reusability (5 circles) and high selectivity (1: 100).
30926029	0	80	theme	glycopeptides	112:124	arg1	identification					85:98	efficient identification	75:98	efficient identification of N-linked glycopeptides and phosphopeptides in human crystalline lens	75:170	l-cysteine-modified metal-organic frameworks as multifunctional probes for efficient identification of N-linked glycopeptides and phosphopeptides in human crystalline lens.
30926029	6	81	from	sites	1595:1599	arg1	surface					1614:1620	the large surface	1604:1620	the large surface of MOFs	1604:1628	The remarkable results were benefitted from the merits of improved hydrophilicity from L-Cys, strong affinity of TiO centers, numerous reaction sites on the large surface of MOFs and superparamagnetism from Fe3O4 cores.
30926029	6	81	from	sites	1595:1599	arg1	merits					1499:1504	the merits	1495:1504	the merits of improved hydrophilicity from L-Cys, strong affinity of TiO centers, numerous reaction sites on the large surface of MOFs and superparamagnetism from Fe3O4 cores	1495:1668	The remarkable results were benefitted from the merits of improved hydrophilicity from L-Cys, strong affinity of TiO centers, numerous reaction sites on the large surface of MOFs and superparamagnetism from Fe3O4 cores.
30926029	6	81	from	sites	1595:1599	arg1	hydrophilicity					1518:1531	improved hydrophilicity	1509:1531	improved hydrophilicity from L-Cys, strong affinity of TiO centers, numerous reaction sites on the large surface of MOFs and superparamagnetism	1509:1651	The remarkable results were benefitted from the merits of improved hydrophilicity from L-Cys, strong affinity of TiO centers, numerous reaction sites on the large surface of MOFs and superparamagnetism from Fe3O4 cores.
30926029	2	82	theme	@	524:524	arg1	L-Cys					525:529	Fe3O4@PDA@MIL-125@Au@L-Cys	504:529	Fe3O4@PDA@MIL-125@Au@L-Cys (mMIL-125@Au@L-Cys)	504:549	In this work, l-cysteine (L-Cys)-modified multifunctional metal-organic frameworks denoted as Fe3O4@PDA@MIL-125@Au@L-Cys (mMIL-125@Au@L-Cys) were prepared by modifications step by step.
30926029	2	82	theme	@	524:524	arg1	L-Cys					544:548	mMIL-125@Au@L-Cys	532:548	mMIL-125@Au@L-Cys	532:548	In this work, l-cysteine (L-Cys)-modified multifunctional metal-organic frameworks denoted as Fe3O4@PDA@MIL-125@Au@L-Cys (mMIL-125@Au@L-Cys) were prepared by modifications step by step.
30926029	3	83	theme	affinity	673:680	arg1	chromatography					682:695	metal oxide affinity chromatography	661:695	metal oxide affinity chromatography (MOAC)	661:702	By combining hydrophilic interaction chromatography (HILIC) with metal oxide affinity chromatography (MOAC), the as-prepared material was firstly utilized to identify N-linked glycopeptides and phosphopeptides from tryptic digests of horseradish peroxidase (HRP) and beta-casein (β-casein), respectively, with the help of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and exhibited outstanding sensitivity (0.1 fmol μL-1), great reusability (5 circles) and high selectivity (1: 100).
30926029	3	83	theme	affinity	673:680	arg1	MOAC					698:701	MOAC	698:701	MOAC	698:701	By combining hydrophilic interaction chromatography (HILIC) with metal oxide affinity chromatography (MOAC), the as-prepared material was firstly utilized to identify N-linked glycopeptides and phosphopeptides from tryptic digests of horseradish peroxidase (HRP) and beta-casein (β-casein), respectively, with the help of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and exhibited outstanding sensitivity (0.1 fmol μL-1), great reusability (5 circles) and high selectivity (1: 100).
30926029	4	84	theme	tryptic	1226:1232	arg1	digests					1234:1240	tryptic digests	1226:1240	tryptic digests of 100 μg human crystalline lens proteins	1226:1282	Based on this, it was further applied into the enrichment of glycopeptides and phosphopeptides from tryptic digests of 100 μg human crystalline lens proteins.
30926029	0	85	theme	phosphopeptides	130:144	arg1	identification					85:98	efficient identification	75:98	efficient identification of N-linked glycopeptides and phosphopeptides in human crystalline lens	75:170	l-cysteine-modified metal-organic frameworks as multifunctional probes for efficient identification of N-linked glycopeptides and phosphopeptides in human crystalline lens.
30926029	2	86	theme	@	543:543	arg1	L-Cys					525:529	Fe3O4@PDA@MIL-125@Au@L-Cys	504:529	Fe3O4@PDA@MIL-125@Au@L-Cys (mMIL-125@Au@L-Cys)	504:549	In this work, l-cysteine (L-Cys)-modified multifunctional metal-organic frameworks denoted as Fe3O4@PDA@MIL-125@Au@L-Cys (mMIL-125@Au@L-Cys) were prepared by modifications step by step.
30926029	2	86	theme	@	543:543	arg1	L-Cys					544:548	mMIL-125@Au@L-Cys	532:548	mMIL-125@Au@L-Cys	532:548	In this work, l-cysteine (L-Cys)-modified multifunctional metal-organic frameworks denoted as Fe3O4@PDA@MIL-125@Au@L-Cys (mMIL-125@Au@L-Cys) were prepared by modifications step by step.
30926029	3	87	gly	glycopeptides	772:784	arg1	digests					819:825	tryptic digests	811:825	tryptic digests of horseradish peroxidase (HRP) and beta-casein (β-casein), respectively	811:898	By combining hydrophilic interaction chromatography (HILIC) with metal oxide affinity chromatography (MOAC), the as-prepared material was firstly utilized to identify N-linked glycopeptides and phosphopeptides from tryptic digests of horseradish peroxidase (HRP) and beta-casein (β-casein), respectively, with the help of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and exhibited outstanding sensitivity (0.1 fmol μL-1), great reusability (5 circles) and high selectivity (1: 100).
30926029	3	87	gly	glycopeptides	772:784	arg2	glycopeptides					772:784	N-linked glycopeptides	763:784	N-linked glycopeptides	763:784	By combining hydrophilic interaction chromatography (HILIC) with metal oxide affinity chromatography (MOAC), the as-prepared material was firstly utilized to identify N-linked glycopeptides and phosphopeptides from tryptic digests of horseradish peroxidase (HRP) and beta-casein (β-casein), respectively, with the help of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and exhibited outstanding sensitivity (0.1 fmol μL-1), great reusability (5 circles) and high selectivity (1: 100).
30926029	6	88	from	affinity	1552:1559	arg1	surface					1614:1620	the large surface	1604:1620	the large surface of MOFs	1604:1628	The remarkable results were benefitted from the merits of improved hydrophilicity from L-Cys, strong affinity of TiO centers, numerous reaction sites on the large surface of MOFs and superparamagnetism from Fe3O4 cores.
30926029	6	88	from	affinity	1552:1559	arg1	merits					1499:1504	the merits	1495:1504	the merits of improved hydrophilicity from L-Cys, strong affinity of TiO centers, numerous reaction sites on the large surface of MOFs and superparamagnetism from Fe3O4 cores	1495:1668	The remarkable results were benefitted from the merits of improved hydrophilicity from L-Cys, strong affinity of TiO centers, numerous reaction sites on the large surface of MOFs and superparamagnetism from Fe3O4 cores.
30926029	6	88	from	affinity	1552:1559	arg1	hydrophilicity					1518:1531	improved hydrophilicity	1509:1531	improved hydrophilicity from L-Cys, strong affinity of TiO centers, numerous reaction sites on the large surface of MOFs and superparamagnetism	1509:1651	The remarkable results were benefitted from the merits of improved hydrophilicity from L-Cys, strong affinity of TiO centers, numerous reaction sites on the large surface of MOFs and superparamagnetism from Fe3O4 cores.
30926029	0	89	theme	human	149:153	arg1	lens					167:170	human crystalline lens	149:170	human crystalline lens	149:170	l-cysteine-modified metal-organic frameworks as multifunctional probes for efficient identification of N-linked glycopeptides and phosphopeptides in human crystalline lens.
30926029	3	90	theme	matrix-assisted	918:932	arg1	spectrometry					982:993	matrix-assisted laser desorption ionization time-of-flight mass spectrometry	918:993	matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS)	918:1008	By combining hydrophilic interaction chromatography (HILIC) with metal oxide affinity chromatography (MOAC), the as-prepared material was firstly utilized to identify N-linked glycopeptides and phosphopeptides from tryptic digests of horseradish peroxidase (HRP) and beta-casein (β-casein), respectively, with the help of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and exhibited outstanding sensitivity (0.1 fmol μL-1), great reusability (5 circles) and high selectivity (1: 100).
30926029	3	90	theme	matrix-assisted	918:932	arg1	MS					1006:1007	MALDI-TOF MS	996:1007	MALDI-TOF MS	996:1007	By combining hydrophilic interaction chromatography (HILIC) with metal oxide affinity chromatography (MOAC), the as-prepared material was firstly utilized to identify N-linked glycopeptides and phosphopeptides from tryptic digests of horseradish peroxidase (HRP) and beta-casein (β-casein), respectively, with the help of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and exhibited outstanding sensitivity (0.1 fmol μL-1), great reusability (5 circles) and high selectivity (1: 100).
30926029	2	91	theme	@	540:540	arg1	L-Cys					525:529	Fe3O4@PDA@MIL-125@Au@L-Cys	504:529	Fe3O4@PDA@MIL-125@Au@L-Cys (mMIL-125@Au@L-Cys)	504:549	In this work, l-cysteine (L-Cys)-modified multifunctional metal-organic frameworks denoted as Fe3O4@PDA@MIL-125@Au@L-Cys (mMIL-125@Au@L-Cys) were prepared by modifications step by step.
30926029	2	91	theme	@	540:540	arg1	L-Cys					544:548	mMIL-125@Au@L-Cys	532:548	mMIL-125@Au@L-Cys	532:548	In this work, l-cysteine (L-Cys)-modified multifunctional metal-organic frameworks denoted as Fe3O4@PDA@MIL-125@Au@L-Cys (mMIL-125@Au@L-Cys) were prepared by modifications step by step.
30926029	4	92	theme	glycopeptides	1187:1199	arg1	enrichment					1173:1182	the enrichment	1169:1182	the enrichment of glycopeptides and phosphopeptides from tryptic digests of 100 μg human crystalline lens proteins	1169:1282	Based on this, it was further applied into the enrichment of glycopeptides and phosphopeptides from tryptic digests of 100 μg human crystalline lens proteins.
30926029	0	93	theme	metal-organic	20:32	arg1	frameworks					34:43	l-cysteine-modified metal-organic frameworks	0:43	l-cysteine-modified metal-organic frameworks as multifunctional probes for efficient identification of N-linked glycopeptides and phosphopeptides in human crystalline lens.	0:171	l-cysteine-modified metal-organic frameworks as multifunctional probes for efficient identification of N-linked glycopeptides and phosphopeptides in human crystalline lens.
30926029	7	94	theme	further	1927:1933	arg1	research					1946:1953	further proteomics research	1927:1953	further proteomics research	1927:1953	The design of mMIL-125@Au@L-Cys not only served as a multifunctional probe for efficient identification of N-linked glycopeptides and phosphopeptides in human crystalline lens, but also set a precedent for fabricating more MOFs with post-modifications for further proteomics research.
30926029	6	95	from	L-Cys	1538:1542	arg1	surface					1614:1620	the large surface	1604:1620	the large surface of MOFs	1604:1628	The remarkable results were benefitted from the merits of improved hydrophilicity from L-Cys, strong affinity of TiO centers, numerous reaction sites on the large surface of MOFs and superparamagnetism from Fe3O4 cores.
30926029	6	95	from	L-Cys	1538:1542	arg1	merits					1499:1504	the merits	1495:1504	the merits of improved hydrophilicity from L-Cys, strong affinity of TiO centers, numerous reaction sites on the large surface of MOFs and superparamagnetism from Fe3O4 cores	1495:1668	The remarkable results were benefitted from the merits of improved hydrophilicity from L-Cys, strong affinity of TiO centers, numerous reaction sites on the large surface of MOFs and superparamagnetism from Fe3O4 cores.
30926029	6	95	from	L-Cys	1538:1542	arg1	hydrophilicity					1518:1531	improved hydrophilicity	1509:1531	improved hydrophilicity from L-Cys, strong affinity of TiO centers, numerous reaction sites on the large surface of MOFs and superparamagnetism	1509:1651	The remarkable results were benefitted from the merits of improved hydrophilicity from L-Cys, strong affinity of TiO centers, numerous reaction sites on the large surface of MOFs and superparamagnetism from Fe3O4 cores.
30926029	7	96	theme	Au	1694:1695	arg1	L-Cys					1697:1701	mMIL-125@Au@L-Cys	1685:1701	mMIL-125@Au@L-Cys	1685:1701	The design of mMIL-125@Au@L-Cys not only served as a multifunctional probe for efficient identification of N-linked glycopeptides and phosphopeptides in human crystalline lens, but also set a precedent for fabricating more MOFs with post-modifications for further proteomics research.
30926029	7	97	from	lens	1842:1845	arg1	identification					1760:1773	efficient identification	1750:1773	efficient identification of N-linked glycopeptides and phosphopeptides in human crystalline lens	1750:1845	The design of mMIL-125@Au@L-Cys not only served as a multifunctional probe for efficient identification of N-linked glycopeptides and phosphopeptides in human crystalline lens, but also set a precedent for fabricating more MOFs with post-modifications for further proteomics research.
30926029	2	98	theme	@	509:509	arg1	L-Cys					525:529	Fe3O4@PDA@MIL-125@Au@L-Cys	504:529	Fe3O4@PDA@MIL-125@Au@L-Cys (mMIL-125@Au@L-Cys)	504:549	In this work, l-cysteine (L-Cys)-modified multifunctional metal-organic frameworks denoted as Fe3O4@PDA@MIL-125@Au@L-Cys (mMIL-125@Au@L-Cys) were prepared by modifications step by step.
30926029	2	98	theme	@	509:509	arg1	L-Cys					544:548	mMIL-125@Au@L-Cys	532:548	mMIL-125@Au@L-Cys	532:548	In this work, l-cysteine (L-Cys)-modified multifunctional metal-organic frameworks denoted as Fe3O4@PDA@MIL-125@Au@L-Cys (mMIL-125@Au@L-Cys) were prepared by modifications step by step.
30926029	4	99	theme	phosphopeptides	1205:1219	arg1	enrichment					1173:1182	the enrichment	1169:1182	the enrichment of glycopeptides and phosphopeptides from tryptic digests of 100 μg human crystalline lens proteins	1169:1282	Based on this, it was further applied into the enrichment of glycopeptides and phosphopeptides from tryptic digests of 100 μg human crystalline lens proteins.
30926029	3	100	theme	time-of-flight	962:975	arg1	spectrometry					982:993	matrix-assisted laser desorption ionization time-of-flight mass spectrometry	918:993	matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS)	918:1008	By combining hydrophilic interaction chromatography (HILIC) with metal oxide affinity chromatography (MOAC), the as-prepared material was firstly utilized to identify N-linked glycopeptides and phosphopeptides from tryptic digests of horseradish peroxidase (HRP) and beta-casein (β-casein), respectively, with the help of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and exhibited outstanding sensitivity (0.1 fmol μL-1), great reusability (5 circles) and high selectivity (1: 100).
30926029	3	100	theme	time-of-flight	962:975	arg1	MS					1006:1007	MALDI-TOF MS	996:1007	MALDI-TOF MS	996:1007	By combining hydrophilic interaction chromatography (HILIC) with metal oxide affinity chromatography (MOAC), the as-prepared material was firstly utilized to identify N-linked glycopeptides and phosphopeptides from tryptic digests of horseradish peroxidase (HRP) and beta-casein (β-casein), respectively, with the help of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and exhibited outstanding sensitivity (0.1 fmol μL-1), great reusability (5 circles) and high selectivity (1: 100).
30926029	1	101	link	N-linked	204:211	arg1	glycopeptides					213:225	N-linked glycopeptides	204:225	N-linked glycopeptides	204:225	Highly selective enrichment of N-linked glycopeptides and phosphopeptides from complex biological samples is extremely important prior to mass spectrometry analysis due to their low abundance as well as numerous extrinsic interferences.
30926029	3	102	theme	metal	661:665	arg1	chromatography					682:695	metal oxide affinity chromatography	661:695	metal oxide affinity chromatography (MOAC)	661:702	By combining hydrophilic interaction chromatography (HILIC) with metal oxide affinity chromatography (MOAC), the as-prepared material was firstly utilized to identify N-linked glycopeptides and phosphopeptides from tryptic digests of horseradish peroxidase (HRP) and beta-casein (β-casein), respectively, with the help of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and exhibited outstanding sensitivity (0.1 fmol μL-1), great reusability (5 circles) and high selectivity (1: 100).
30926029	3	102	theme	metal	661:665	arg1	MOAC					698:701	MOAC	698:701	MOAC	698:701	By combining hydrophilic interaction chromatography (HILIC) with metal oxide affinity chromatography (MOAC), the as-prepared material was firstly utilized to identify N-linked glycopeptides and phosphopeptides from tryptic digests of horseradish peroxidase (HRP) and beta-casein (β-casein), respectively, with the help of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and exhibited outstanding sensitivity (0.1 fmol μL-1), great reusability (5 circles) and high selectivity (1: 100).
30926029	5	103	theme	81 N-linked	1297:1307	arg1	glycopeptides					1309:1321	81 N-linked glycopeptides	1297:1321	81 N-linked glycopeptides corresponding to 35 glycoproteins and 175 phosphopeptides ascribed to 55 phosphorylated proteins	1297:1418	In the end, 81 N-linked glycopeptides corresponding to 35 glycoproteins and 175 phosphopeptides ascribed to 55 phosphorylated proteins were identified, respectively.
30926029	7	104	theme	L-Cys	1697:1701	arg1	precedent					1863:1871	a precedent	1861:1871	a precedent for fabricating more MOFs with post-modifications for further proteomics research	1861:1953	The design of mMIL-125@Au@L-Cys not only served as a multifunctional probe for efficient identification of N-linked glycopeptides and phosphopeptides in human crystalline lens, but also set a precedent for fabricating more MOFs with post-modifications for further proteomics research.
30926029	7	104	theme	L-Cys	1697:1701	arg1	probe					1740:1744	a multifunctional probe	1722:1744	a multifunctional probe for efficient identification of N-linked glycopeptides and phosphopeptides in human crystalline lens	1722:1845	The design of mMIL-125@Au@L-Cys not only served as a multifunctional probe for efficient identification of N-linked glycopeptides and phosphopeptides in human crystalline lens, but also set a precedent for fabricating more MOFs with post-modifications for further proteomics research.
30926029	7	104	theme	L-Cys	1697:1701	arg1	design					1675:1680	The design	1671:1680	The design of mMIL-125@Au@L-Cys	1671:1701	The design of mMIL-125@Au@L-Cys not only served as a multifunctional probe for efficient identification of N-linked glycopeptides and phosphopeptides in human crystalline lens, but also set a precedent for fabricating more MOFs with post-modifications for further proteomics research.
30926029	3	105	theme	desorption	940:949	arg1	spectrometry					982:993	matrix-assisted laser desorption ionization time-of-flight mass spectrometry	918:993	matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS)	918:1008	By combining hydrophilic interaction chromatography (HILIC) with metal oxide affinity chromatography (MOAC), the as-prepared material was firstly utilized to identify N-linked glycopeptides and phosphopeptides from tryptic digests of horseradish peroxidase (HRP) and beta-casein (β-casein), respectively, with the help of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and exhibited outstanding sensitivity (0.1 fmol μL-1), great reusability (5 circles) and high selectivity (1: 100).
30926029	3	105	theme	desorption	940:949	arg1	MS					1006:1007	MALDI-TOF MS	996:1007	MALDI-TOF MS	996:1007	By combining hydrophilic interaction chromatography (HILIC) with metal oxide affinity chromatography (MOAC), the as-prepared material was firstly utilized to identify N-linked glycopeptides and phosphopeptides from tryptic digests of horseradish peroxidase (HRP) and beta-casein (β-casein), respectively, with the help of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and exhibited outstanding sensitivity (0.1 fmol μL-1), great reusability (5 circles) and high selectivity (1: 100).
30926029	2	106	theme	@	521:521	arg1	L-Cys					525:529	Fe3O4@PDA@MIL-125@Au@L-Cys	504:529	Fe3O4@PDA@MIL-125@Au@L-Cys (mMIL-125@Au@L-Cys)	504:549	In this work, l-cysteine (L-Cys)-modified multifunctional metal-organic frameworks denoted as Fe3O4@PDA@MIL-125@Au@L-Cys (mMIL-125@Au@L-Cys) were prepared by modifications step by step.
30926029	2	106	theme	@	521:521	arg1	L-Cys					544:548	mMIL-125@Au@L-Cys	532:548	mMIL-125@Au@L-Cys	532:548	In this work, l-cysteine (L-Cys)-modified multifunctional metal-organic frameworks denoted as Fe3O4@PDA@MIL-125@Au@L-Cys (mMIL-125@Au@L-Cys) were prepared by modifications step by step.
30926029	2	107	theme	multifunctional	452:466	arg1	frameworks					482:491	l-cysteine (L-Cys)-modified multifunctional metal-organic frameworks	424:491	l-cysteine (L-Cys)-modified multifunctional metal-organic frameworks denoted as Fe3O4@PDA@MIL-125@Au@L-Cys (mMIL-125@Au@L-Cys)	424:549	In this work, l-cysteine (L-Cys)-modified multifunctional metal-organic frameworks denoted as Fe3O4@PDA@MIL-125@Au@L-Cys (mMIL-125@Au@L-Cys) were prepared by modifications step by step.
30926029	0	108	theme	efficient	75:83	arg1	identification					85:98	efficient identification	75:98	efficient identification of N-linked glycopeptides and phosphopeptides in human crystalline lens	75:170	l-cysteine-modified metal-organic frameworks as multifunctional probes for efficient identification of N-linked glycopeptides and phosphopeptides in human crystalline lens.
30926029	3	109	theme	MALDI-TOF	996:1004	arg1	spectrometry					982:993	matrix-assisted laser desorption ionization time-of-flight mass spectrometry	918:993	matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS)	918:1008	By combining hydrophilic interaction chromatography (HILIC) with metal oxide affinity chromatography (MOAC), the as-prepared material was firstly utilized to identify N-linked glycopeptides and phosphopeptides from tryptic digests of horseradish peroxidase (HRP) and beta-casein (β-casein), respectively, with the help of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and exhibited outstanding sensitivity (0.1 fmol μL-1), great reusability (5 circles) and high selectivity (1: 100).
30926029	3	109	theme	MALDI-TOF	996:1004	arg1	MS					1006:1007	MALDI-TOF MS	996:1007	MALDI-TOF MS	996:1007	By combining hydrophilic interaction chromatography (HILIC) with metal oxide affinity chromatography (MOAC), the as-prepared material was firstly utilized to identify N-linked glycopeptides and phosphopeptides from tryptic digests of horseradish peroxidase (HRP) and beta-casein (β-casein), respectively, with the help of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and exhibited outstanding sensitivity (0.1 fmol μL-1), great reusability (5 circles) and high selectivity (1: 100).
30926029	2	110	theme	@	513:513	arg1	L-Cys					525:529	Fe3O4@PDA@MIL-125@Au@L-Cys	504:529	Fe3O4@PDA@MIL-125@Au@L-Cys (mMIL-125@Au@L-Cys)	504:549	In this work, l-cysteine (L-Cys)-modified multifunctional metal-organic frameworks denoted as Fe3O4@PDA@MIL-125@Au@L-Cys (mMIL-125@Au@L-Cys) were prepared by modifications step by step.
30926029	2	110	theme	@	513:513	arg1	L-Cys					544:548	mMIL-125@Au@L-Cys	532:548	mMIL-125@Au@L-Cys	532:548	In this work, l-cysteine (L-Cys)-modified multifunctional metal-organic frameworks denoted as Fe3O4@PDA@MIL-125@Au@L-Cys (mMIL-125@Au@L-Cys) were prepared by modifications step by step.
30926029	4	111	theme	human	1252:1256	arg1	proteins					1275:1282	100 μg human crystalline lens proteins	1245:1282	100 μg human crystalline lens proteins	1245:1282	Based on this, it was further applied into the enrichment of glycopeptides and phosphopeptides from tryptic digests of 100 μg human crystalline lens proteins.
30926029	5	112	theme	phosphorylated	1396:1409	arg1	proteins					1411:1418	55 phosphorylated proteins	1393:1418	55 phosphorylated proteins	1393:1418	In the end, 81 N-linked glycopeptides corresponding to 35 glycoproteins and 175 phosphopeptides ascribed to 55 phosphorylated proteins were identified, respectively.
30926029	6	113	theme	hydrophilicity	1518:1531	arg1	merits					1499:1504	the merits	1495:1504	the merits of improved hydrophilicity from L-Cys, strong affinity of TiO centers, numerous reaction sites on the large surface of MOFs and superparamagnetism from Fe3O4 cores	1495:1668	The remarkable results were benefitted from the merits of improved hydrophilicity from L-Cys, strong affinity of TiO centers, numerous reaction sites on the large surface of MOFs and superparamagnetism from Fe3O4 cores.
30926029	1	114	theme	numerous	376:383	arg1	interferences					395:407	numerous extrinsic interferences	376:407	their low abundance as well as numerous extrinsic interferences	345:407	Highly selective enrichment of N-linked glycopeptides and phosphopeptides from complex biological samples is extremely important prior to mass spectrometry analysis due to their low abundance as well as numerous extrinsic interferences.
30926029	4	115	theme	lens	1270:1273	arg1	proteins					1275:1282	100 μg human crystalline lens proteins	1245:1282	100 μg human crystalline lens proteins	1245:1282	Based on this, it was further applied into the enrichment of glycopeptides and phosphopeptides from tryptic digests of 100 μg human crystalline lens proteins.
30926029	3	116	dep	the	906:908	arg1	help					910:913	help	910:913	help	910:913	By combining hydrophilic interaction chromatography (HILIC) with metal oxide affinity chromatography (MOAC), the as-prepared material was firstly utilized to identify N-linked glycopeptides and phosphopeptides from tryptic digests of horseradish peroxidase (HRP) and beta-casein (β-casein), respectively, with the help of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and exhibited outstanding sensitivity (0.1 fmol μL-1), great reusability (5 circles) and high selectivity (1: 100).
30926029	7	117	from	glycopeptides	1787:1799	arg1	lens					1842:1845	human crystalline lens	1824:1845	human crystalline lens	1824:1845	The design of mMIL-125@Au@L-Cys not only served as a multifunctional probe for efficient identification of N-linked glycopeptides and phosphopeptides in human crystalline lens, but also set a precedent for fabricating more MOFs with post-modifications for further proteomics research.
30926029	7	118	link	N-linked	1778:1785	arg1	glycopeptides					1787:1799	N-linked glycopeptides	1778:1799	N-linked glycopeptides	1778:1799	The design of mMIL-125@Au@L-Cys not only served as a multifunctional probe for efficient identification of N-linked glycopeptides and phosphopeptides in human crystalline lens, but also set a precedent for fabricating more MOFs with post-modifications for further proteomics research.
30457839	5	0	gly	glycosites	1020:1029	arg2	glycosites					1020:1029	1162 unique glycosites	1008:1029	1162 unique glycosites in 530 glycoproteins, including 71 unique atypical N-linked IGPs on 18 atypical N-glycosylation sequons with an overrepresentation of the N-X-C motifs	1008:1180	A total of 10 338 N-linked glycosite-containing IGPs were identified, representing 1162 unique glycosites in 530 glycoproteins, including 71 unique atypical N-linked IGPs on 18 atypical N-glycosylation sequons with an overrepresentation of the N-X-C motifs.
30457839	2	1	theme	proteins	329:336	arg1	effects					416:422	side effects	411:422	side effects	411:422	The characterization glycosylation profile of therapeutic proteins produced from engineered CHO cells and therapeutic functions, as well as side effects, are critical to understand the important roles of glycosylation.
30457839	2	1	theme	proteins	329:336	arg1	critical					429:436	critical	429:436	critical	429:436	The characterization glycosylation profile of therapeutic proteins produced from engineered CHO cells and therapeutic functions, as well as side effects, are critical to understand the important roles of glycosylation.
30457839	2	1	theme	proteins	329:336	arg1	profile					306:312	The characterization glycosylation profile	271:312	The characterization glycosylation profile of therapeutic proteins produced from engineered CHO cells and therapeutic functions	271:397	The characterization glycosylation profile of therapeutic proteins produced from engineered CHO cells and therapeutic functions, as well as side effects, are critical to understand the important roles of glycosylation.
30457839	7	2	theme	genomic	1433:1439	arg1	data					1475:1478	the genomic, proteomic, and N-linked glycomic data	1429:1478	the genomic, proteomic, and N-linked glycomic data previously reported for CHO cells	1429:1512	The obtained large scale glycoproteomic data from intact N-linked glycopeptides in this study is complementary to the genomic, proteomic, and N-linked glycomic data previously reported for CHO cells.
30457839	4	3	gly	glycopeptides	646:658	arg2	glycopeptides					646:658	intact glycopeptides	639:658	intact glycopeptides from CHO-K1 cell lysate and medium using hydrophilic enrichment, fractionation of the obtained intact glycopeptides (IGPs) by basic reversed phase liquid chromatography (bRPLC), analyzing the glycopeptides using LC-MS/MS, and annotating the results by GPQuest 2.0	639:922	The workflow includes enrichment of intact glycopeptides from CHO-K1 cell lysate and medium using hydrophilic enrichment, fractionation of the obtained intact glycopeptides (IGPs) by basic reversed phase liquid chromatography (bRPLC), analyzing the glycopeptides using LC-MS/MS, and annotating the results by GPQuest 2.0.
30457839	4	3	gly	glycopeptides	646:658	arg1	medium					688:693	medium	688:693	medium	688:693	The workflow includes enrichment of intact glycopeptides from CHO-K1 cell lysate and medium using hydrophilic enrichment, fractionation of the obtained intact glycopeptides (IGPs) by basic reversed phase liquid chromatography (bRPLC), analyzing the glycopeptides using LC-MS/MS, and annotating the results by GPQuest 2.0.
30457839	4	3	gly	glycopeptides	646:658	arg1	lysate					677:682	CHO-K1 cell lysate	665:682	CHO-K1 cell lysate	665:682	The workflow includes enrichment of intact glycopeptides from CHO-K1 cell lysate and medium using hydrophilic enrichment, fractionation of the obtained intact glycopeptides (IGPs) by basic reversed phase liquid chromatography (bRPLC), analyzing the glycopeptides using LC-MS/MS, and annotating the results by GPQuest 2.0.
30457839	3	4	theme	large	507:511	arg1	workflow					534:541	a large scale glycoproteomic workflow	505:541	a large scale glycoproteomic workflow	505:541	In this study, a large scale glycoproteomic workflow was established and applied to CHO-K1 cells expressing EPO.
30457839	7	5	theme	N-linked	1457:1464	arg1	data					1475:1478	the genomic, proteomic, and N-linked glycomic data	1429:1478	the genomic, proteomic, and N-linked glycomic data previously reported for CHO cells	1429:1512	The obtained large scale glycoproteomic data from intact N-linked glycopeptides in this study is complementary to the genomic, proteomic, and N-linked glycomic data previously reported for CHO cells.
30457839	6	6	gly	glycoproteins	1209:1221	arg1	glycoproteins					1209:1221	the glycoproteins	1205:1221	the glycoproteins from CHO cell lysate	1205:1242	Moreover, we compared the glycoproteins from CHO cell lysate with those from medium using the in-depth N-linked glycoproteome data.
30457839	5	7	gly	N-glycosylation	1111:1125	arg2	sequons					1127:1133	18 atypical N-glycosylation sequons	1099:1133	18 atypical N-glycosylation sequons with an overrepresentation of the N-X-C motifs	1099:1180	A total of 10 338 N-linked glycosite-containing IGPs were identified, representing 1162 unique glycosites in 530 glycoproteins, including 71 unique atypical N-linked IGPs on 18 atypical N-glycosylation sequons with an overrepresentation of the N-X-C motifs.
30457839	4	8	theme	glycopeptides	646:658	arg1	enrichment					625:634	enrichment	625:634	enrichment of intact glycopeptides from CHO-K1 cell lysate and medium using hydrophilic enrichment, fractionation of the obtained intact glycopeptides (IGPs) by basic reversed phase liquid chromatography (bRPLC), analyzing the glycopeptides using LC-MS/MS, and annotating the results by GPQuest 2.0	625:922	The workflow includes enrichment of intact glycopeptides from CHO-K1 cell lysate and medium using hydrophilic enrichment, fractionation of the obtained intact glycopeptides (IGPs) by basic reversed phase liquid chromatography (bRPLC), analyzing the glycopeptides using LC-MS/MS, and annotating the results by GPQuest 2.0.
30457839	6	9	theme	cell	1232:1235	arg1	lysate					1237:1242	CHO cell lysate	1228:1242	CHO cell lysate	1228:1242	Moreover, we compared the glycoproteins from CHO cell lysate with those from medium using the in-depth N-linked glycoproteome data.
30457839	1	10	theme	therapeutic	163:173	arg1	proteins					175:182	therapeutic proteins	163:182	therapeutic proteins	163:182	The Chinese hamster ovary (CHO) cell line is a major expression system for the production of therapeutic proteins, the majority of which are glycoproteins, such as antibodies and erythropoietin (EPO).
30457839	3	11	theme	glycoproteomic	519:532	arg1	workflow					534:541	a large scale glycoproteomic workflow	505:541	a large scale glycoproteomic workflow	505:541	In this study, a large scale glycoproteomic workflow was established and applied to CHO-K1 cells expressing EPO.
30457839	6	12	theme	CHO	1228:1230	arg1	lysate					1237:1242	CHO cell lysate	1228:1242	CHO cell lysate	1228:1242	Moreover, we compared the glycoproteins from CHO cell lysate with those from medium using the in-depth N-linked glycoproteome data.
30457839	7	13	theme	intact	1365:1370	arg1	glycopeptides					1381:1393	intact N-linked glycopeptides	1365:1393	intact N-linked glycopeptides	1365:1393	The obtained large scale glycoproteomic data from intact N-linked glycopeptides in this study is complementary to the genomic, proteomic, and N-linked glycomic data previously reported for CHO cells.
30457839	6	14	theme	glycoproteome	1295:1307	arg1	data					1309:1312	the in-depth N-linked glycoproteome data	1273:1312	the in-depth N-linked glycoproteome data	1273:1312	Moreover, we compared the glycoproteins from CHO cell lysate with those from medium using the in-depth N-linked glycoproteome data.
30457839	4	15	theme	hydrophilic	701:711	arg1	fractionation					725:737	fractionation	725:737	fractionation of the obtained intact glycopeptides (IGPs) by basic reversed phase liquid chromatography (bRPLC)	725:835	The workflow includes enrichment of intact glycopeptides from CHO-K1 cell lysate and medium using hydrophilic enrichment, fractionation of the obtained intact glycopeptides (IGPs) by basic reversed phase liquid chromatography (bRPLC), analyzing the glycopeptides using LC-MS/MS, and annotating the results by GPQuest 2.0.
30457839	4	15	theme	hydrophilic	701:711	arg1	enrichment					713:722	hydrophilic enrichment	701:722	hydrophilic enrichment	701:722	The workflow includes enrichment of intact glycopeptides from CHO-K1 cell lysate and medium using hydrophilic enrichment, fractionation of the obtained intact glycopeptides (IGPs) by basic reversed phase liquid chromatography (bRPLC), analyzing the glycopeptides using LC-MS/MS, and annotating the results by GPQuest 2.0.
30457839	6	16	theme	in-depth	1277:1284	arg1	data					1309:1312	the in-depth N-linked glycoproteome data	1273:1312	the in-depth N-linked glycoproteome data	1273:1312	Moreover, we compared the glycoproteins from CHO cell lysate with those from medium using the in-depth N-linked glycoproteome data.
30457839	5	17	theme	530	1034:1036	arg1	IGPs					1091:1094	71 unique atypical N-linked IGPs	1063:1094	71 unique atypical N-linked IGPs on 18 atypical N-glycosylation sequons with an overrepresentation of the N-X-C motifs	1063:1180	A total of 10 338 N-linked glycosite-containing IGPs were identified, representing 1162 unique glycosites in 530 glycoproteins, including 71 unique atypical N-linked IGPs on 18 atypical N-glycosylation sequons with an overrepresentation of the N-X-C motifs.
30457839	5	17	theme	530	1034:1036	arg1	glycoproteins					1038:1050	530 glycoproteins	1034:1050	530 glycoproteins	1034:1050	A total of 10 338 N-linked glycosite-containing IGPs were identified, representing 1162 unique glycosites in 530 glycoproteins, including 71 unique atypical N-linked IGPs on 18 atypical N-glycosylation sequons with an overrepresentation of the N-X-C motifs.
30457839	7	18	theme	scale	1334:1338	arg1	data					1355:1358	The obtained large scale glycoproteomic data	1315:1358	The obtained large scale glycoproteomic data from intact N-linked glycopeptides in this study	1315:1407	The obtained large scale glycoproteomic data from intact N-linked glycopeptides in this study is complementary to the genomic, proteomic, and N-linked glycomic data previously reported for CHO cells.
30457839	7	18	theme	scale	1334:1338	arg1	complementary					1412:1424	complementary	1412:1424	complementary	1412:1424	The obtained large scale glycoproteomic data from intact N-linked glycopeptides in this study is complementary to the genomic, proteomic, and N-linked glycomic data previously reported for CHO cells.
30457839	4	19	theme	CHO-K1	665:670	arg1	lysate					677:682	CHO-K1 cell lysate	665:682	CHO-K1 cell lysate	665:682	The workflow includes enrichment of intact glycopeptides from CHO-K1 cell lysate and medium using hydrophilic enrichment, fractionation of the obtained intact glycopeptides (IGPs) by basic reversed phase liquid chromatography (bRPLC), analyzing the glycopeptides using LC-MS/MS, and annotating the results by GPQuest 2.0.
30457839	1	20	theme	expression	123:132	arg1	system					134:139	a major expression system	115:139	a major expression system for the production of therapeutic proteins, the majority of which are glycoproteins, such as antibodies and erythropoietin (EPO)	115:268	The Chinese hamster ovary (CHO) cell line is a major expression system for the production of therapeutic proteins, the majority of which are glycoproteins, such as antibodies and erythropoietin (EPO).
30457839	1	20	theme	expression	123:132	arg1	line					107:110	The Chinese hamster ovary (CHO) cell line	70:110	The Chinese hamster ovary (CHO) cell line	70:110	The Chinese hamster ovary (CHO) cell line is a major expression system for the production of therapeutic proteins, the majority of which are glycoproteins, such as antibodies and erythropoietin (EPO).
30457839	2	21	theme	important	456:464	arg1	roles					466:470	the important roles	452:470	the important roles of glycosylation	452:487	The characterization glycosylation profile of therapeutic proteins produced from engineered CHO cells and therapeutic functions, as well as side effects, are critical to understand the important roles of glycosylation.
30457839	5	22	with	sequons	1127:1133	arg1	overrepresentation					1143:1160	an overrepresentation	1140:1160	an overrepresentation of the N-X-C motifs	1140:1180	A total of 10 338 N-linked glycosite-containing IGPs were identified, representing 1162 unique glycosites in 530 glycoproteins, including 71 unique atypical N-linked IGPs on 18 atypical N-glycosylation sequons with an overrepresentation of the N-X-C motifs.
30457839	4	23	gly	glycopeptides	762:774	arg2	glycopeptides					762:774	the obtained intact glycopeptides	742:774	the obtained intact glycopeptides (IGPs)	742:781	The workflow includes enrichment of intact glycopeptides from CHO-K1 cell lysate and medium using hydrophilic enrichment, fractionation of the obtained intact glycopeptides (IGPs) by basic reversed phase liquid chromatography (bRPLC), analyzing the glycopeptides using LC-MS/MS, and annotating the results by GPQuest 2.0.
30457839	4	23	gly	glycopeptides	762:774	arg2	IGPs					777:780	IGPs	777:780	IGPs	777:780	The workflow includes enrichment of intact glycopeptides from CHO-K1 cell lysate and medium using hydrophilic enrichment, fractionation of the obtained intact glycopeptides (IGPs) by basic reversed phase liquid chromatography (bRPLC), analyzing the glycopeptides using LC-MS/MS, and annotating the results by GPQuest 2.0.
30457839	5	24	theme	N-glycosylation	1111:1125	arg1	sequons					1127:1133	18 atypical N-glycosylation sequons	1099:1133	18 atypical N-glycosylation sequons with an overrepresentation of the N-X-C motifs	1099:1180	A total of 10 338 N-linked glycosite-containing IGPs were identified, representing 1162 unique glycosites in 530 glycoproteins, including 71 unique atypical N-linked IGPs on 18 atypical N-glycosylation sequons with an overrepresentation of the N-X-C motifs.
30457839	4	25	theme	obtained	746:753	arg1	IGPs					777:780	IGPs	777:780	IGPs	777:780	The workflow includes enrichment of intact glycopeptides from CHO-K1 cell lysate and medium using hydrophilic enrichment, fractionation of the obtained intact glycopeptides (IGPs) by basic reversed phase liquid chromatography (bRPLC), analyzing the glycopeptides using LC-MS/MS, and annotating the results by GPQuest 2.0.
30457839	4	25	theme	obtained	746:753	arg1	glycopeptides					762:774	the obtained intact glycopeptides	742:774	the obtained intact glycopeptides (IGPs)	742:781	The workflow includes enrichment of intact glycopeptides from CHO-K1 cell lysate and medium using hydrophilic enrichment, fractionation of the obtained intact glycopeptides (IGPs) by basic reversed phase liquid chromatography (bRPLC), analyzing the glycopeptides using LC-MS/MS, and annotating the results by GPQuest 2.0.
30457839	5	26	theme	atypical	1073:1080	arg1	IGPs					1091:1094	71 unique atypical N-linked IGPs	1063:1094	71 unique atypical N-linked IGPs on 18 atypical N-glycosylation sequons with an overrepresentation of the N-X-C motifs	1063:1180	A total of 10 338 N-linked glycosite-containing IGPs were identified, representing 1162 unique glycosites in 530 glycoproteins, including 71 unique atypical N-linked IGPs on 18 atypical N-glycosylation sequons with an overrepresentation of the N-X-C motifs.
30457839	1	27	gly	glycoproteins	211:223	arg1	glycoproteins					211:223	glycoproteins	211:223	glycoproteins	211:223	The Chinese hamster ovary (CHO) cell line is a major expression system for the production of therapeutic proteins, the majority of which are glycoproteins, such as antibodies and erythropoietin (EPO).
30457839	1	27	gly	glycoproteins	211:223	arg1	erythropoietin					249:262	erythropoietin	249:262	erythropoietin (EPO)	249:268	The Chinese hamster ovary (CHO) cell line is a major expression system for the production of therapeutic proteins, the majority of which are glycoproteins, such as antibodies and erythropoietin (EPO).
30457839	1	27	gly	glycoproteins	211:223	arg1	antibodies					234:243	antibodies	234:243	antibodies	234:243	The Chinese hamster ovary (CHO) cell line is a major expression system for the production of therapeutic proteins, the majority of which are glycoproteins, such as antibodies and erythropoietin (EPO).
30457839	1	27	gly	glycoproteins	211:223	arg1	majority					189:196	the majority	185:196	the majority	185:196	The Chinese hamster ovary (CHO) cell line is a major expression system for the production of therapeutic proteins, the majority of which are glycoproteins, such as antibodies and erythropoietin (EPO).
30457839	4	28	theme	glycopeptides	762:774	arg1	fractionation					725:737	fractionation	725:737	fractionation of the obtained intact glycopeptides (IGPs) by basic reversed phase liquid chromatography (bRPLC)	725:835	The workflow includes enrichment of intact glycopeptides from CHO-K1 cell lysate and medium using hydrophilic enrichment, fractionation of the obtained intact glycopeptides (IGPs) by basic reversed phase liquid chromatography (bRPLC), analyzing the glycopeptides using LC-MS/MS, and annotating the results by GPQuest 2.0.
30457839	4	28	theme	glycopeptides	762:774	arg1	enrichment					713:722	hydrophilic enrichment	701:722	hydrophilic enrichment	701:722	The workflow includes enrichment of intact glycopeptides from CHO-K1 cell lysate and medium using hydrophilic enrichment, fractionation of the obtained intact glycopeptides (IGPs) by basic reversed phase liquid chromatography (bRPLC), analyzing the glycopeptides using LC-MS/MS, and annotating the results by GPQuest 2.0.
30457839	2	29	theme	therapeutic	377:387	arg1	functions					389:397	therapeutic functions	377:397	therapeutic functions	377:397	The characterization glycosylation profile of therapeutic proteins produced from engineered CHO cells and therapeutic functions, as well as side effects, are critical to understand the important roles of glycosylation.
30457839	5	30	theme	N-linked	943:950	arg1	IGPs					973:976	10 338 N-linked glycosite-containing IGPs	936:976	10 338 N-linked glycosite-containing IGPs	936:976	A total of 10 338 N-linked glycosite-containing IGPs were identified, representing 1162 unique glycosites in 530 glycoproteins, including 71 unique atypical N-linked IGPs on 18 atypical N-glycosylation sequons with an overrepresentation of the N-X-C motifs.
30457839	7	31	from	data	1355:1358	arg1	study					1403:1407	this study	1398:1407	this study	1398:1407	The obtained large scale glycoproteomic data from intact N-linked glycopeptides in this study is complementary to the genomic, proteomic, and N-linked glycomic data previously reported for CHO cells.
30457839	7	32	theme	large	1328:1332	arg1	data					1355:1358	The obtained large scale glycoproteomic data	1315:1358	The obtained large scale glycoproteomic data from intact N-linked glycopeptides in this study	1315:1407	The obtained large scale glycoproteomic data from intact N-linked glycopeptides in this study is complementary to the genomic, proteomic, and N-linked glycomic data previously reported for CHO cells.
30457839	7	32	theme	large	1328:1332	arg1	complementary					1412:1424	complementary	1412:1424	complementary	1412:1424	The obtained large scale glycoproteomic data from intact N-linked glycopeptides in this study is complementary to the genomic, proteomic, and N-linked glycomic data previously reported for CHO cells.
30457839	0	33	theme	Comprehensive	0:12	arg1	Analysis					29:36	Comprehensive Glycoproteomic Analysis	0:36	Comprehensive Glycoproteomic Analysis of Chinese Hamster Ovary Cells	0:67	Comprehensive Glycoproteomic Analysis of Chinese Hamster Ovary Cells.
30457839	2	34	theme	side	411:414	arg1	effects					416:422	side effects	411:422	side effects	411:422	The characterization glycosylation profile of therapeutic proteins produced from engineered CHO cells and therapeutic functions, as well as side effects, are critical to understand the important roles of glycosylation.
30457839	5	35	theme	motifs	1175:1180	arg1	overrepresentation					1143:1160	an overrepresentation	1140:1160	an overrepresentation of the N-X-C motifs	1140:1180	A total of 10 338 N-linked glycosite-containing IGPs were identified, representing 1162 unique glycosites in 530 glycoproteins, including 71 unique atypical N-linked IGPs on 18 atypical N-glycosylation sequons with an overrepresentation of the N-X-C motifs.
30457839	7	36	from	glycopeptides	1381:1393	arg1	data					1355:1358	The obtained large scale glycoproteomic data	1315:1358	The obtained large scale glycoproteomic data from intact N-linked glycopeptides in this study	1315:1407	The obtained large scale glycoproteomic data from intact N-linked glycopeptides in this study is complementary to the genomic, proteomic, and N-linked glycomic data previously reported for CHO cells.
30457839	7	36	from	glycopeptides	1381:1393	arg1	complementary					1412:1424	complementary	1412:1424	complementary	1412:1424	The obtained large scale glycoproteomic data from intact N-linked glycopeptides in this study is complementary to the genomic, proteomic, and N-linked glycomic data previously reported for CHO cells.
30457839	4	37	theme	reversed	792:799	arg1	bRPLC					830:834	bRPLC	830:834	bRPLC	830:834	The workflow includes enrichment of intact glycopeptides from CHO-K1 cell lysate and medium using hydrophilic enrichment, fractionation of the obtained intact glycopeptides (IGPs) by basic reversed phase liquid chromatography (bRPLC), analyzing the glycopeptides using LC-MS/MS, and annotating the results by GPQuest 2.0.
30457839	4	37	theme	reversed	792:799	arg1	chromatography					814:827	basic reversed phase liquid chromatography	786:827	basic reversed phase liquid chromatography (bRPLC)	786:835	The workflow includes enrichment of intact glycopeptides from CHO-K1 cell lysate and medium using hydrophilic enrichment, fractionation of the obtained intact glycopeptides (IGPs) by basic reversed phase liquid chromatography (bRPLC), analyzing the glycopeptides using LC-MS/MS, and annotating the results by GPQuest 2.0.
30457839	0	38	theme	Chinese	41:47	arg1	Cells					63:67	Chinese Hamster Ovary Cells	41:67	Chinese Hamster Ovary Cells	41:67	Comprehensive Glycoproteomic Analysis of Chinese Hamster Ovary Cells.
30457839	4	39	theme	liquid	807:812	arg1	bRPLC					830:834	bRPLC	830:834	bRPLC	830:834	The workflow includes enrichment of intact glycopeptides from CHO-K1 cell lysate and medium using hydrophilic enrichment, fractionation of the obtained intact glycopeptides (IGPs) by basic reversed phase liquid chromatography (bRPLC), analyzing the glycopeptides using LC-MS/MS, and annotating the results by GPQuest 2.0.
30457839	4	39	theme	liquid	807:812	arg1	chromatography					814:827	basic reversed phase liquid chromatography	786:827	basic reversed phase liquid chromatography (bRPLC)	786:835	The workflow includes enrichment of intact glycopeptides from CHO-K1 cell lysate and medium using hydrophilic enrichment, fractionation of the obtained intact glycopeptides (IGPs) by basic reversed phase liquid chromatography (bRPLC), analyzing the glycopeptides using LC-MS/MS, and annotating the results by GPQuest 2.0.
30457839	2	40	theme	therapeutic	317:327	arg1	proteins					329:336	therapeutic proteins	317:336	therapeutic proteins produced from engineered CHO cells and therapeutic functions	317:397	The characterization glycosylation profile of therapeutic proteins produced from engineered CHO cells and therapeutic functions, as well as side effects, are critical to understand the important roles of glycosylation.
30457839	0	41	theme	Ovary	57:61	arg1	Cells					63:67	Chinese Hamster Ovary Cells	41:67	Chinese Hamster Ovary Cells	41:67	Comprehensive Glycoproteomic Analysis of Chinese Hamster Ovary Cells.
30457839	5	42	theme	unique	1013:1018	arg1	glycosites					1020:1029	1162 unique glycosites	1008:1029	1162 unique glycosites in 530 glycoproteins, including 71 unique atypical N-linked IGPs on 18 atypical N-glycosylation sequons with an overrepresentation of the N-X-C motifs	1008:1180	A total of 10 338 N-linked glycosite-containing IGPs were identified, representing 1162 unique glycosites in 530 glycoproteins, including 71 unique atypical N-linked IGPs on 18 atypical N-glycosylation sequons with an overrepresentation of the N-X-C motifs.
30457839	8	43	theme	recombinant	1573:1583	arg1	glycoproteins					1597:1609	recombinant therapeutic glycoproteins	1573:1609	recombinant therapeutic glycoproteins	1573:1609	Our method has the potential to monitor the production of recombinant therapeutic glycoproteins.
30457839	2	44	gly	glycosylation	292:304	arg1	proteins					329:336	therapeutic proteins	317:336	therapeutic proteins produced from engineered CHO cells and therapeutic functions	317:397	The characterization glycosylation profile of therapeutic proteins produced from engineered CHO cells and therapeutic functions, as well as side effects, are critical to understand the important roles of glycosylation.
30457839	5	45	theme	IGPs	973:976	arg1	total					927:931	A total	925:931	A total of 10 338 N-linked glycosite-containing IGPs	925:976	A total of 10 338 N-linked glycosite-containing IGPs were identified, representing 1162 unique glycosites in 530 glycoproteins, including 71 unique atypical N-linked IGPs on 18 atypical N-glycosylation sequons with an overrepresentation of the N-X-C motifs.
30457839	8	46	theme	glycoproteins	1597:1609	arg1	production					1559:1568	the production	1555:1568	the production of recombinant therapeutic glycoproteins	1555:1609	Our method has the potential to monitor the production of recombinant therapeutic glycoproteins.
30457839	2	47	theme	engineered	352:361	arg1	cells					367:371	engineered CHO cells	352:371	engineered CHO cells	352:371	The characterization glycosylation profile of therapeutic proteins produced from engineered CHO cells and therapeutic functions, as well as side effects, are critical to understand the important roles of glycosylation.
30457839	6	48	link	N-linked	1286:1293	arg1	data					1309:1312	the in-depth N-linked glycoproteome data	1273:1312	the in-depth N-linked glycoproteome data	1273:1312	Moreover, we compared the glycoproteins from CHO cell lysate with those from medium using the in-depth N-linked glycoproteome data.
30457839	6	49	from	lysate	1237:1242	arg1	glycoproteins					1209:1221	the glycoproteins	1205:1221	the glycoproteins from CHO cell lysate	1205:1242	Moreover, we compared the glycoproteins from CHO cell lysate with those from medium using the in-depth N-linked glycoproteome data.
30457839	7	50	theme	glycomic	1466:1473	arg1	data					1475:1478	the genomic, proteomic, and N-linked glycomic data	1429:1478	the genomic, proteomic, and N-linked glycomic data previously reported for CHO cells	1429:1512	The obtained large scale glycoproteomic data from intact N-linked glycopeptides in this study is complementary to the genomic, proteomic, and N-linked glycomic data previously reported for CHO cells.
30457839	5	51	gly	glycoproteins	1038:1050	arg1	IGPs					1091:1094	71 unique atypical N-linked IGPs	1063:1094	71 unique atypical N-linked IGPs on 18 atypical N-glycosylation sequons with an overrepresentation of the N-X-C motifs	1063:1180	A total of 10 338 N-linked glycosite-containing IGPs were identified, representing 1162 unique glycosites in 530 glycoproteins, including 71 unique atypical N-linked IGPs on 18 atypical N-glycosylation sequons with an overrepresentation of the N-X-C motifs.
30457839	5	51	gly	glycoproteins	1038:1050	arg1	glycoproteins					1038:1050	530 glycoproteins	1034:1050	530 glycoproteins	1034:1050	A total of 10 338 N-linked glycosite-containing IGPs were identified, representing 1162 unique glycosites in 530 glycoproteins, including 71 unique atypical N-linked IGPs on 18 atypical N-glycosylation sequons with an overrepresentation of the N-X-C motifs.
30457839	5	52	link	N-linked	943:950	arg1	IGPs					973:976	10 338 N-linked glycosite-containing IGPs	936:976	10 338 N-linked glycosite-containing IGPs	936:976	A total of 10 338 N-linked glycosite-containing IGPs were identified, representing 1162 unique glycosites in 530 glycoproteins, including 71 unique atypical N-linked IGPs on 18 atypical N-glycosylation sequons with an overrepresentation of the N-X-C motifs.
30457839	8	53	contain	has	1526:1528	arg1	method					1519:1524	Our method	1515:1524	Our method	1515:1524	Our method has the potential to monitor the production of recombinant therapeutic glycoproteins.
30457839	8	53	contain	has	1526:1528	arg2	potential					1534:1542	the potential to monitor the production of recombinant therapeutic glycoproteins	1530:1609	the potential to monitor the production of recombinant therapeutic glycoproteins	1530:1609	Our method has the potential to monitor the production of recombinant therapeutic glycoproteins.
30457839	7	54	link	N-linked	1457:1464	arg1	data					1475:1478	the genomic, proteomic, and N-linked glycomic data	1429:1478	the genomic, proteomic, and N-linked glycomic data previously reported for CHO cells	1429:1512	The obtained large scale glycoproteomic data from intact N-linked glycopeptides in this study is complementary to the genomic, proteomic, and N-linked glycomic data previously reported for CHO cells.
30457839	7	55	theme	proteomic	1442:1450	arg1	data					1475:1478	the genomic, proteomic, and N-linked glycomic data	1429:1478	the genomic, proteomic, and N-linked glycomic data previously reported for CHO cells	1429:1512	The obtained large scale glycoproteomic data from intact N-linked glycopeptides in this study is complementary to the genomic, proteomic, and N-linked glycomic data previously reported for CHO cells.
30457839	2	56	theme	characterization	275:290	arg1	critical					429:436	critical	429:436	critical	429:436	The characterization glycosylation profile of therapeutic proteins produced from engineered CHO cells and therapeutic functions, as well as side effects, are critical to understand the important roles of glycosylation.
30457839	2	56	theme	characterization	275:290	arg1	profile					306:312	The characterization glycosylation profile	271:312	The characterization glycosylation profile of therapeutic proteins produced from engineered CHO cells and therapeutic functions	271:397	The characterization glycosylation profile of therapeutic proteins produced from engineered CHO cells and therapeutic functions, as well as side effects, are critical to understand the important roles of glycosylation.
30457839	4	57	from	enrichment	625:634	arg1	medium					688:693	medium	688:693	medium	688:693	The workflow includes enrichment of intact glycopeptides from CHO-K1 cell lysate and medium using hydrophilic enrichment, fractionation of the obtained intact glycopeptides (IGPs) by basic reversed phase liquid chromatography (bRPLC), analyzing the glycopeptides using LC-MS/MS, and annotating the results by GPQuest 2.0.
30457839	4	57	from	enrichment	625:634	arg1	lysate					677:682	CHO-K1 cell lysate	665:682	CHO-K1 cell lysate	665:682	The workflow includes enrichment of intact glycopeptides from CHO-K1 cell lysate and medium using hydrophilic enrichment, fractionation of the obtained intact glycopeptides (IGPs) by basic reversed phase liquid chromatography (bRPLC), analyzing the glycopeptides using LC-MS/MS, and annotating the results by GPQuest 2.0.
30457839	4	58	theme	intact	639:644	arg1	glycopeptides					646:658	intact glycopeptides	639:658	intact glycopeptides from CHO-K1 cell lysate and medium using hydrophilic enrichment, fractionation of the obtained intact glycopeptides (IGPs) by basic reversed phase liquid chromatography (bRPLC), analyzing the glycopeptides using LC-MS/MS, and annotating the results by GPQuest 2.0	639:922	The workflow includes enrichment of intact glycopeptides from CHO-K1 cell lysate and medium using hydrophilic enrichment, fractionation of the obtained intact glycopeptides (IGPs) by basic reversed phase liquid chromatography (bRPLC), analyzing the glycopeptides using LC-MS/MS, and annotating the results by GPQuest 2.0.
30457839	3	59	theme	scale	513:517	arg1	workflow					534:541	a large scale glycoproteomic workflow	505:541	a large scale glycoproteomic workflow	505:541	In this study, a large scale glycoproteomic workflow was established and applied to CHO-K1 cells expressing EPO.
30457839	1	60	theme	proteins	175:182	arg1	erythropoietin					249:262	erythropoietin	249:262	erythropoietin (EPO)	249:268	The Chinese hamster ovary (CHO) cell line is a major expression system for the production of therapeutic proteins, the majority of which are glycoproteins, such as antibodies and erythropoietin (EPO).
30457839	1	60	theme	proteins	175:182	arg1	glycoproteins					211:223	glycoproteins	211:223	glycoproteins	211:223	The Chinese hamster ovary (CHO) cell line is a major expression system for the production of therapeutic proteins, the majority of which are glycoproteins, such as antibodies and erythropoietin (EPO).
30457839	1	60	theme	proteins	175:182	arg1	production					149:158	the production	145:158	the production of therapeutic proteins, the majority of which are glycoproteins, such as antibodies and erythropoietin (EPO)	145:268	The Chinese hamster ovary (CHO) cell line is a major expression system for the production of therapeutic proteins, the majority of which are glycoproteins, such as antibodies and erythropoietin (EPO).
30457839	1	60	theme	proteins	175:182	arg1	antibodies					234:243	antibodies	234:243	antibodies	234:243	The Chinese hamster ovary (CHO) cell line is a major expression system for the production of therapeutic proteins, the majority of which are glycoproteins, such as antibodies and erythropoietin (EPO).
30457839	1	60	theme	proteins	175:182	arg1	majority					189:196	the majority	185:196	the majority	185:196	The Chinese hamster ovary (CHO) cell line is a major expression system for the production of therapeutic proteins, the majority of which are glycoproteins, such as antibodies and erythropoietin (EPO).
30457839	4	61	gly	glycopeptides	852:864	arg2	glycopeptides					852:864	the glycopeptides	848:864	the glycopeptides using LC-MS/MS	848:879	The workflow includes enrichment of intact glycopeptides from CHO-K1 cell lysate and medium using hydrophilic enrichment, fractionation of the obtained intact glycopeptides (IGPs) by basic reversed phase liquid chromatography (bRPLC), analyzing the glycopeptides using LC-MS/MS, and annotating the results by GPQuest 2.0.
30457839	7	62	gly	glycopeptides	1381:1393	arg2	glycopeptides					1381:1393	intact N-linked glycopeptides	1365:1393	intact N-linked glycopeptides	1365:1393	The obtained large scale glycoproteomic data from intact N-linked glycopeptides in this study is complementary to the genomic, proteomic, and N-linked glycomic data previously reported for CHO cells.
30457839	5	63	theme	unique	1066:1071	arg1	IGPs					1091:1094	71 unique atypical N-linked IGPs	1063:1094	71 unique atypical N-linked IGPs on 18 atypical N-glycosylation sequons with an overrepresentation of the N-X-C motifs	1063:1180	A total of 10 338 N-linked glycosite-containing IGPs were identified, representing 1162 unique glycosites in 530 glycoproteins, including 71 unique atypical N-linked IGPs on 18 atypical N-glycosylation sequons with an overrepresentation of the N-X-C motifs.
30457839	1	64	theme	cell	102:105	arg1	system					134:139	a major expression system	115:139	a major expression system for the production of therapeutic proteins, the majority of which are glycoproteins, such as antibodies and erythropoietin (EPO)	115:268	The Chinese hamster ovary (CHO) cell line is a major expression system for the production of therapeutic proteins, the majority of which are glycoproteins, such as antibodies and erythropoietin (EPO).
30457839	1	64	theme	cell	102:105	arg1	line					107:110	The Chinese hamster ovary (CHO) cell line	70:110	The Chinese hamster ovary (CHO) cell line	70:110	The Chinese hamster ovary (CHO) cell line is a major expression system for the production of therapeutic proteins, the majority of which are glycoproteins, such as antibodies and erythropoietin (EPO).
30457839	7	65	theme	N-linked	1372:1379	arg1	glycopeptides					1381:1393	intact N-linked glycopeptides	1365:1393	intact N-linked glycopeptides	1365:1393	The obtained large scale glycoproteomic data from intact N-linked glycopeptides in this study is complementary to the genomic, proteomic, and N-linked glycomic data previously reported for CHO cells.
30457839	6	66	theme	N-linked	1286:1293	arg1	data					1309:1312	the in-depth N-linked glycoproteome data	1273:1312	the in-depth N-linked glycoproteome data	1273:1312	Moreover, we compared the glycoproteins from CHO cell lysate with those from medium using the in-depth N-linked glycoproteome data.
30457839	3	67	theme	CHO-K1	574:579	arg1	cells					581:585	CHO-K1 cells	574:585	CHO-K1 cells expressing EPO	574:600	In this study, a large scale glycoproteomic workflow was established and applied to CHO-K1 cells expressing EPO.
30457839	1	68	theme	major	117:121	arg1	system					134:139	a major expression system	115:139	a major expression system for the production of therapeutic proteins, the majority of which are glycoproteins, such as antibodies and erythropoietin (EPO)	115:268	The Chinese hamster ovary (CHO) cell line is a major expression system for the production of therapeutic proteins, the majority of which are glycoproteins, such as antibodies and erythropoietin (EPO).
30457839	1	68	theme	major	117:121	arg1	line					107:110	The Chinese hamster ovary (CHO) cell line	70:110	The Chinese hamster ovary (CHO) cell line	70:110	The Chinese hamster ovary (CHO) cell line is a major expression system for the production of therapeutic proteins, the majority of which are glycoproteins, such as antibodies and erythropoietin (EPO).
30457839	7	69	theme	glycoproteomic	1340:1353	arg1	data					1355:1358	The obtained large scale glycoproteomic data	1315:1358	The obtained large scale glycoproteomic data from intact N-linked glycopeptides in this study	1315:1407	The obtained large scale glycoproteomic data from intact N-linked glycopeptides in this study is complementary to the genomic, proteomic, and N-linked glycomic data previously reported for CHO cells.
30457839	7	69	theme	glycoproteomic	1340:1353	arg1	complementary					1412:1424	complementary	1412:1424	complementary	1412:1424	The obtained large scale glycoproteomic data from intact N-linked glycopeptides in this study is complementary to the genomic, proteomic, and N-linked glycomic data previously reported for CHO cells.
30457839	5	70	from	glycosites	1020:1029	arg1	IGPs					1091:1094	71 unique atypical N-linked IGPs	1063:1094	71 unique atypical N-linked IGPs on 18 atypical N-glycosylation sequons with an overrepresentation of the N-X-C motifs	1063:1180	A total of 10 338 N-linked glycosite-containing IGPs were identified, representing 1162 unique glycosites in 530 glycoproteins, including 71 unique atypical N-linked IGPs on 18 atypical N-glycosylation sequons with an overrepresentation of the N-X-C motifs.
30457839	5	70	from	glycosites	1020:1029	arg1	glycoproteins					1038:1050	530 glycoproteins	1034:1050	530 glycoproteins	1034:1050	A total of 10 338 N-linked glycosite-containing IGPs were identified, representing 1162 unique glycosites in 530 glycoproteins, including 71 unique atypical N-linked IGPs on 18 atypical N-glycosylation sequons with an overrepresentation of the N-X-C motifs.
30457839	5	71	theme	atypical	1102:1109	arg1	sequons					1127:1133	18 atypical N-glycosylation sequons	1099:1133	18 atypical N-glycosylation sequons with an overrepresentation of the N-X-C motifs	1099:1180	A total of 10 338 N-linked glycosite-containing IGPs were identified, representing 1162 unique glycosites in 530 glycoproteins, including 71 unique atypical N-linked IGPs on 18 atypical N-glycosylation sequons with an overrepresentation of the N-X-C motifs.
30457839	4	72	theme	cell	672:675	arg1	lysate					677:682	CHO-K1 cell lysate	665:682	CHO-K1 cell lysate	665:682	The workflow includes enrichment of intact glycopeptides from CHO-K1 cell lysate and medium using hydrophilic enrichment, fractionation of the obtained intact glycopeptides (IGPs) by basic reversed phase liquid chromatography (bRPLC), analyzing the glycopeptides using LC-MS/MS, and annotating the results by GPQuest 2.0.
30457839	4	73	dep	lysate	677:682	arg1	analyzing					838:846	analyzing	838:846	analyzing the glycopeptides using LC-MS/MS	838:879	The workflow includes enrichment of intact glycopeptides from CHO-K1 cell lysate and medium using hydrophilic enrichment, fractionation of the obtained intact glycopeptides (IGPs) by basic reversed phase liquid chromatography (bRPLC), analyzing the glycopeptides using LC-MS/MS, and annotating the results by GPQuest 2.0.
30457839	4	73	dep	lysate	677:682	arg1	using					695:699	using	695:699	using hydrophilic enrichment, fractionation of the obtained intact glycopeptides (IGPs) by basic reversed phase liquid chromatography (bRPLC)	695:835	The workflow includes enrichment of intact glycopeptides from CHO-K1 cell lysate and medium using hydrophilic enrichment, fractionation of the obtained intact glycopeptides (IGPs) by basic reversed phase liquid chromatography (bRPLC), analyzing the glycopeptides using LC-MS/MS, and annotating the results by GPQuest 2.0.
30457839	4	73	dep	lysate	677:682	arg1	annotating					886:895	annotating	886:895	annotating the results by GPQuest 2.0	886:922	The workflow includes enrichment of intact glycopeptides from CHO-K1 cell lysate and medium using hydrophilic enrichment, fractionation of the obtained intact glycopeptides (IGPs) by basic reversed phase liquid chromatography (bRPLC), analyzing the glycopeptides using LC-MS/MS, and annotating the results by GPQuest 2.0.
30457839	5	74	theme	N-linked	1082:1089	arg1	IGPs					1091:1094	71 unique atypical N-linked IGPs	1063:1094	71 unique atypical N-linked IGPs on 18 atypical N-glycosylation sequons with an overrepresentation of the N-X-C motifs	1063:1180	A total of 10 338 N-linked glycosite-containing IGPs were identified, representing 1162 unique glycosites in 530 glycoproteins, including 71 unique atypical N-linked IGPs on 18 atypical N-glycosylation sequons with an overrepresentation of the N-X-C motifs.
30457839	1	75	theme	Chinese	74:80	arg1	CHO					97:99	CHO	97:99	CHO	97:99	The Chinese hamster ovary (CHO) cell line is a major expression system for the production of therapeutic proteins, the majority of which are glycoproteins, such as antibodies and erythropoietin (EPO).
30457839	1	75	theme	Chinese	74:80	arg1	ovary					90:94	Chinese hamster ovary	74:94	The Chinese hamster ovary (CHO) cell line	70:110	The Chinese hamster ovary (CHO) cell line is a major expression system for the production of therapeutic proteins, the majority of which are glycoproteins, such as antibodies and erythropoietin (EPO).
30457839	7	76	theme	obtained	1319:1326	arg1	data					1355:1358	The obtained large scale glycoproteomic data	1315:1358	The obtained large scale glycoproteomic data from intact N-linked glycopeptides in this study	1315:1407	The obtained large scale glycoproteomic data from intact N-linked glycopeptides in this study is complementary to the genomic, proteomic, and N-linked glycomic data previously reported for CHO cells.
30457839	7	76	theme	obtained	1319:1326	arg1	complementary					1412:1424	complementary	1412:1424	complementary	1412:1424	The obtained large scale glycoproteomic data from intact N-linked glycopeptides in this study is complementary to the genomic, proteomic, and N-linked glycomic data previously reported for CHO cells.
30457839	2	77	theme	glycosylation	475:487	arg1	roles					466:470	the important roles	452:470	the important roles of glycosylation	452:487	The characterization glycosylation profile of therapeutic proteins produced from engineered CHO cells and therapeutic functions, as well as side effects, are critical to understand the important roles of glycosylation.
30457839	4	78	theme	intact	755:760	arg1	IGPs					777:780	IGPs	777:780	IGPs	777:780	The workflow includes enrichment of intact glycopeptides from CHO-K1 cell lysate and medium using hydrophilic enrichment, fractionation of the obtained intact glycopeptides (IGPs) by basic reversed phase liquid chromatography (bRPLC), analyzing the glycopeptides using LC-MS/MS, and annotating the results by GPQuest 2.0.
30457839	4	78	theme	intact	755:760	arg1	glycopeptides					762:774	the obtained intact glycopeptides	742:774	the obtained intact glycopeptides (IGPs)	742:781	The workflow includes enrichment of intact glycopeptides from CHO-K1 cell lysate and medium using hydrophilic enrichment, fractionation of the obtained intact glycopeptides (IGPs) by basic reversed phase liquid chromatography (bRPLC), analyzing the glycopeptides using LC-MS/MS, and annotating the results by GPQuest 2.0.
30457839	0	79	theme	Glycoproteomic	14:27	arg1	Analysis					29:36	Comprehensive Glycoproteomic Analysis	0:36	Comprehensive Glycoproteomic Analysis of Chinese Hamster Ovary Cells	0:67	Comprehensive Glycoproteomic Analysis of Chinese Hamster Ovary Cells.
30457839	1	80	theme	ovary	90:94	arg1	system					134:139	a major expression system	115:139	a major expression system for the production of therapeutic proteins, the majority of which are glycoproteins, such as antibodies and erythropoietin (EPO)	115:268	The Chinese hamster ovary (CHO) cell line is a major expression system for the production of therapeutic proteins, the majority of which are glycoproteins, such as antibodies and erythropoietin (EPO).
30457839	1	80	theme	ovary	90:94	arg1	line					107:110	The Chinese hamster ovary (CHO) cell line	70:110	The Chinese hamster ovary (CHO) cell line	70:110	The Chinese hamster ovary (CHO) cell line is a major expression system for the production of therapeutic proteins, the majority of which are glycoproteins, such as antibodies and erythropoietin (EPO).
30457839	5	81	link	N-linked	1082:1089	arg1	IGPs					1091:1094	71 unique atypical N-linked IGPs	1063:1094	71 unique atypical N-linked IGPs on 18 atypical N-glycosylation sequons with an overrepresentation of the N-X-C motifs	1063:1180	A total of 10 338 N-linked glycosite-containing IGPs were identified, representing 1162 unique glycosites in 530 glycoproteins, including 71 unique atypical N-linked IGPs on 18 atypical N-glycosylation sequons with an overrepresentation of the N-X-C motifs.
30457839	2	82	theme	CHO	363:365	arg1	cells					367:371	engineered CHO cells	352:371	engineered CHO cells	352:371	The characterization glycosylation profile of therapeutic proteins produced from engineered CHO cells and therapeutic functions, as well as side effects, are critical to understand the important roles of glycosylation.
30457839	5	83	theme	N-X-C	1169:1173	arg1	motifs					1175:1180	the N-X-C motifs	1165:1180	the N-X-C motifs	1165:1180	A total of 10 338 N-linked glycosite-containing IGPs were identified, representing 1162 unique glycosites in 530 glycoproteins, including 71 unique atypical N-linked IGPs on 18 atypical N-glycosylation sequons with an overrepresentation of the N-X-C motifs.
30457839	0	84	theme	Hamster	49:55	arg1	Cells					63:67	Chinese Hamster Ovary Cells	41:67	Chinese Hamster Ovary Cells	41:67	Comprehensive Glycoproteomic Analysis of Chinese Hamster Ovary Cells.
30457839	8	85	gly	glycoproteins	1597:1609	arg1	glycoproteins					1597:1609	recombinant therapeutic glycoproteins	1573:1609	recombinant therapeutic glycoproteins	1573:1609	Our method has the potential to monitor the production of recombinant therapeutic glycoproteins.
30457839	7	86	link	N-linked	1372:1379	arg1	glycopeptides					1381:1393	intact N-linked glycopeptides	1365:1393	intact N-linked glycopeptides	1365:1393	The obtained large scale glycoproteomic data from intact N-linked glycopeptides in this study is complementary to the genomic, proteomic, and N-linked glycomic data previously reported for CHO cells.
30457839	4	87	theme	basic	786:790	arg1	bRPLC					830:834	bRPLC	830:834	bRPLC	830:834	The workflow includes enrichment of intact glycopeptides from CHO-K1 cell lysate and medium using hydrophilic enrichment, fractionation of the obtained intact glycopeptides (IGPs) by basic reversed phase liquid chromatography (bRPLC), analyzing the glycopeptides using LC-MS/MS, and annotating the results by GPQuest 2.0.
30457839	4	87	theme	basic	786:790	arg1	chromatography					814:827	basic reversed phase liquid chromatography	786:827	basic reversed phase liquid chromatography (bRPLC)	786:835	The workflow includes enrichment of intact glycopeptides from CHO-K1 cell lysate and medium using hydrophilic enrichment, fractionation of the obtained intact glycopeptides (IGPs) by basic reversed phase liquid chromatography (bRPLC), analyzing the glycopeptides using LC-MS/MS, and annotating the results by GPQuest 2.0.
30457839	0	88	theme	Cells	63:67	arg1	Analysis					29:36	Comprehensive Glycoproteomic Analysis	0:36	Comprehensive Glycoproteomic Analysis of Chinese Hamster Ovary Cells	0:67	Comprehensive Glycoproteomic Analysis of Chinese Hamster Ovary Cells.
30457839	8	89	theme	therapeutic	1585:1595	arg1	glycoproteins					1597:1609	recombinant therapeutic glycoproteins	1573:1609	recombinant therapeutic glycoproteins	1573:1609	Our method has the potential to monitor the production of recombinant therapeutic glycoproteins.
30457839	4	90	theme	phase	801:805	arg1	bRPLC					830:834	bRPLC	830:834	bRPLC	830:834	The workflow includes enrichment of intact glycopeptides from CHO-K1 cell lysate and medium using hydrophilic enrichment, fractionation of the obtained intact glycopeptides (IGPs) by basic reversed phase liquid chromatography (bRPLC), analyzing the glycopeptides using LC-MS/MS, and annotating the results by GPQuest 2.0.
30457839	4	90	theme	phase	801:805	arg1	chromatography					814:827	basic reversed phase liquid chromatography	786:827	basic reversed phase liquid chromatography (bRPLC)	786:835	The workflow includes enrichment of intact glycopeptides from CHO-K1 cell lysate and medium using hydrophilic enrichment, fractionation of the obtained intact glycopeptides (IGPs) by basic reversed phase liquid chromatography (bRPLC), analyzing the glycopeptides using LC-MS/MS, and annotating the results by GPQuest 2.0.
30457839	5	91	theme	glycosite-containing	952:971	arg1	IGPs					973:976	10 338 N-linked glycosite-containing IGPs	936:976	10 338 N-linked glycosite-containing IGPs	936:976	A total of 10 338 N-linked glycosite-containing IGPs were identified, representing 1162 unique glycosites in 530 glycoproteins, including 71 unique atypical N-linked IGPs on 18 atypical N-glycosylation sequons with an overrepresentation of the N-X-C motifs.
30457839	1	92	theme	hamster	82:88	arg1	CHO					97:99	CHO	97:99	CHO	97:99	The Chinese hamster ovary (CHO) cell line is a major expression system for the production of therapeutic proteins, the majority of which are glycoproteins, such as antibodies and erythropoietin (EPO).
30457839	1	92	theme	hamster	82:88	arg1	ovary					90:94	Chinese hamster ovary	74:94	The Chinese hamster ovary (CHO) cell line	70:110	The Chinese hamster ovary (CHO) cell line is a major expression system for the production of therapeutic proteins, the majority of which are glycoproteins, such as antibodies and erythropoietin (EPO).
30457839	2	93	theme	glycosylation	292:304	arg1	critical					429:436	critical	429:436	critical	429:436	The characterization glycosylation profile of therapeutic proteins produced from engineered CHO cells and therapeutic functions, as well as side effects, are critical to understand the important roles of glycosylation.
30457839	2	93	theme	glycosylation	292:304	arg1	profile					306:312	The characterization glycosylation profile	271:312	The characterization glycosylation profile of therapeutic proteins produced from engineered CHO cells and therapeutic functions	271:397	The characterization glycosylation profile of therapeutic proteins produced from engineered CHO cells and therapeutic functions, as well as side effects, are critical to understand the important roles of glycosylation.
30457839	4	94	from	medium	688:693	arg1	enrichment					625:634	enrichment	625:634	enrichment of intact glycopeptides from CHO-K1 cell lysate and medium using hydrophilic enrichment, fractionation of the obtained intact glycopeptides (IGPs) by basic reversed phase liquid chromatography (bRPLC), analyzing the glycopeptides using LC-MS/MS, and annotating the results by GPQuest 2.0	625:922	The workflow includes enrichment of intact glycopeptides from CHO-K1 cell lysate and medium using hydrophilic enrichment, fractionation of the obtained intact glycopeptides (IGPs) by basic reversed phase liquid chromatography (bRPLC), analyzing the glycopeptides using LC-MS/MS, and annotating the results by GPQuest 2.0.
30457839	4	94	from	medium	688:693	arg1	glycopeptides					646:658	intact glycopeptides	639:658	intact glycopeptides from CHO-K1 cell lysate and medium using hydrophilic enrichment, fractionation of the obtained intact glycopeptides (IGPs) by basic reversed phase liquid chromatography (bRPLC), analyzing the glycopeptides using LC-MS/MS, and annotating the results by GPQuest 2.0	639:922	The workflow includes enrichment of intact glycopeptides from CHO-K1 cell lysate and medium using hydrophilic enrichment, fractionation of the obtained intact glycopeptides (IGPs) by basic reversed phase liquid chromatography (bRPLC), analyzing the glycopeptides using LC-MS/MS, and annotating the results by GPQuest 2.0.
30457839	7	95	theme	CHO	1504:1506	arg1	cells					1508:1512	CHO cells	1504:1512	CHO cells	1504:1512	The obtained large scale glycoproteomic data from intact N-linked glycopeptides in this study is complementary to the genomic, proteomic, and N-linked glycomic data previously reported for CHO cells.
30457839	5	96	from	IGPs	1091:1094	arg1	sequons					1127:1133	18 atypical N-glycosylation sequons	1099:1133	18 atypical N-glycosylation sequons with an overrepresentation of the N-X-C motifs	1099:1180	A total of 10 338 N-linked glycosite-containing IGPs were identified, representing 1162 unique glycosites in 530 glycoproteins, including 71 unique atypical N-linked IGPs on 18 atypical N-glycosylation sequons with an overrepresentation of the N-X-C motifs.
30457839	4	97	from	lysate	677:682	arg1	enrichment					625:634	enrichment	625:634	enrichment of intact glycopeptides from CHO-K1 cell lysate and medium using hydrophilic enrichment, fractionation of the obtained intact glycopeptides (IGPs) by basic reversed phase liquid chromatography (bRPLC), analyzing the glycopeptides using LC-MS/MS, and annotating the results by GPQuest 2.0	625:922	The workflow includes enrichment of intact glycopeptides from CHO-K1 cell lysate and medium using hydrophilic enrichment, fractionation of the obtained intact glycopeptides (IGPs) by basic reversed phase liquid chromatography (bRPLC), analyzing the glycopeptides using LC-MS/MS, and annotating the results by GPQuest 2.0.
30457839	4	97	from	lysate	677:682	arg1	glycopeptides					646:658	intact glycopeptides	639:658	intact glycopeptides from CHO-K1 cell lysate and medium using hydrophilic enrichment, fractionation of the obtained intact glycopeptides (IGPs) by basic reversed phase liquid chromatography (bRPLC), analyzing the glycopeptides using LC-MS/MS, and annotating the results by GPQuest 2.0	639:922	The workflow includes enrichment of intact glycopeptides from CHO-K1 cell lysate and medium using hydrophilic enrichment, fractionation of the obtained intact glycopeptides (IGPs) by basic reversed phase liquid chromatography (bRPLC), analyzing the glycopeptides using LC-MS/MS, and annotating the results by GPQuest 2.0.
29971627	12	0	theme	breast	1871:1876	arg1	cells					1885:1889	breast cancer cells	1871:1889	breast cancer cells	1871:1889	CONCLUSION N-linked glycosylation interference and glycolysis disruption both contribute to the anticancer properties of 2-DG in breast cancer cells.
29971627	7	1	theme	MDA-MB-231	1290:1299	arg1	cells					1301:1305	2-DG incubated MDA-MB-231 cells	1275:1305	2-DG incubated MDA-MB-231 cells	1275:1305	These results are reinforced by the appearance of an abnormal Man7GlcNAc2 structure both on lipid-linked oligosaccharides and N-linked glycoproteins of 2-DG incubated MDA-MB-231 cells.
29971627	11	2	theme	anti-cancerous	1674:1687	arg1	effects					1689:1695	2-DG anti-cancerous effects	1669:1695	2-DG anti-cancerous effects in MDA-MB-231 and MCF-7 cells	1669:1725	Mannose partially reversed the effects of 2-DG on cell numbers with N-linked glycosylation interference accounting for 37 and 47% of 2-DG anti-cancerous effects in MDA-MB-231 and MCF-7 cells, respectively.
29971627	11	3	theme	cell	1586:1589	arg1	numbers					1591:1597	cell numbers	1586:1597	cell numbers with N-linked glycosylation interference accounting for 37 and 47% of 2-DG anti-cancerous effects in MDA-MB-231 and MCF-7 cells, respectively	1586:1739	Mannose partially reversed the effects of 2-DG on cell numbers with N-linked glycosylation interference accounting for 37 and 47% of 2-DG anti-cancerous effects in MDA-MB-231 and MCF-7 cells, respectively.
29971627	1	4	theme	inhibitors	270:279	arg1	development					244:254	the development	240:254	the development of glycolysis inhibitors	240:279	PURPOSE Cancer cells often elicit a higher glycolytic rate than normal cells, supporting the development of glycolysis inhibitors as therapeutic agents.
29971627	1	4	theme	inhibitors	270:279	arg1	agents					296:301	therapeutic agents	284:301	therapeutic agents	284:301	PURPOSE Cancer cells often elicit a higher glycolytic rate than normal cells, supporting the development of glycolysis inhibitors as therapeutic agents.
29971627	4	5	link	N-linked	698:705	arg1	interference					721:732	N-linked glycosylation interference	698:732	N-linked glycosylation interference	698:732	Since there are limited publications on 2-DG mechanism of action in breast cancer, we studied its effects in breast cancer cell lines to determine the part played by glycolysis inhibition and N-linked glycosylation interference.
29971627	11	6	theme	2-DG	1578:1581	arg1	effects					1567:1573	the effects	1563:1573	the effects of 2-DG on cell numbers with N-linked glycosylation interference accounting for 37 and 47% of 2-DG anti-cancerous effects in MDA-MB-231 and MCF-7 cells, respectively	1563:1739	Mannose partially reversed the effects of 2-DG on cell numbers with N-linked glycosylation interference accounting for 37 and 47% of 2-DG anti-cancerous effects in MDA-MB-231 and MCF-7 cells, respectively.
29971627	1	7	theme	therapeutic	284:294	arg1	development					244:254	the development	240:254	the development of glycolysis inhibitors	240:279	PURPOSE Cancer cells often elicit a higher glycolytic rate than normal cells, supporting the development of glycolysis inhibitors as therapeutic agents.
29971627	1	7	theme	therapeutic	284:294	arg1	agents					296:301	therapeutic agents	284:301	therapeutic agents	284:301	PURPOSE Cancer cells often elicit a higher glycolytic rate than normal cells, supporting the development of glycolysis inhibitors as therapeutic agents.
29971627	11	8	theme	glycosylation	1613:1625	arg1	interference					1627:1638	N-linked glycosylation interference	1604:1638	N-linked glycosylation interference accounting for 37 and 47% of 2-DG anti-cancerous effects in MDA-MB-231 and MCF-7 cells, respectively	1604:1739	Mannose partially reversed the effects of 2-DG on cell numbers with N-linked glycosylation interference accounting for 37 and 47% of 2-DG anti-cancerous effects in MDA-MB-231 and MCF-7 cells, respectively.
29971627	7	9	link	N-linked	1249:1256	arg1	glycoproteins					1258:1270	N-linked glycoproteins	1249:1270	N-linked glycoproteins	1249:1270	These results are reinforced by the appearance of an abnormal Man7GlcNAc2 structure both on lipid-linked oligosaccharides and N-linked glycoproteins of 2-DG incubated MDA-MB-231 cells.
29971627	1	10	theme	higher	187:192	arg1	rate					205:208	a higher glycolytic rate	185:208	a higher glycolytic rate than normal cells	185:226	PURPOSE Cancer cells often elicit a higher glycolytic rate than normal cells, supporting the development of glycolysis inhibitors as therapeutic agents.
29971627	8	11	theme	2-DG-induced	1317:1328	arg1	stress					1364:1369	2-DG-induced a transient endoplasmic reticulum stress	1317:1369	2-DG-induced a transient endoplasmic reticulum stress that was more sustained in MDA-MB-231 cells	1317:1413	Besides, 2-DG-induced a transient endoplasmic reticulum stress that was more sustained in MDA-MB-231 cells.
29971627	0	12	theme	cancer	137:142	arg1	cells					144:148	breast cancer cells	130:148	breast cancer cells	130:148	Protein N-glycosylation alteration and glycolysis inhibition both contribute to the antiproliferative action of 2-deoxyglucose in breast cancer cells.
29971627	6	13	theme	calumenin	1112:1120	arg1	profile					1053:1059	the migration profile	1039:1059	the migration profile of the lysosomal-associated membrane protein 2 and calumenin	1039:1120	It also interfered with the N-linked glycosylation process in both cell lines as illustrated by the migration profile of the lysosomal-associated membrane protein 2 and calumenin.
29971627	5	14	theme	cell	830:833	arg1	number					835:840	cell number decrease	830:849	cell number decrease	830:849	METHODS AND RESULTS 2-Deoxyglucose behaved as an anticancer agent with a similar efficiency on cell number decrease between the hormone-dependent MCF-7 and hormone-independent MDA-MB-231 breast cancer cells.
29971627	6	15	theme	N-linked	971:978	arg1	process					994:1000	the N-linked glycosylation process	967:1000	the N-linked glycosylation process in both cell lines	967:1019	It also interfered with the N-linked glycosylation process in both cell lines as illustrated by the migration profile of the lysosomal-associated membrane protein 2 and calumenin.
29971627	8	16	theme	transient	1332:1340	arg1	reticulum					1354:1362	a transient endoplasmic reticulum	1330:1362	2-DG-induced a transient endoplasmic reticulum stress that was more sustained in MDA-MB-231 cells	1317:1413	Besides, 2-DG-induced a transient endoplasmic reticulum stress that was more sustained in MDA-MB-231 cells.
29971627	6	17	link	N-linked	971:978	arg1	process					994:1000	the N-linked glycosylation process	967:1000	the N-linked glycosylation process in both cell lines	967:1019	It also interfered with the N-linked glycosylation process in both cell lines as illustrated by the migration profile of the lysosomal-associated membrane protein 2 and calumenin.
29971627	8	18	theme	reticulum	1354:1362	arg1	stress					1364:1369	2-DG-induced a transient endoplasmic reticulum stress	1317:1369	2-DG-induced a transient endoplasmic reticulum stress that was more sustained in MDA-MB-231 cells	1317:1413	Besides, 2-DG-induced a transient endoplasmic reticulum stress that was more sustained in MDA-MB-231 cells.
29971627	4	19	theme	N-linked	698:705	arg1	interference					721:732	N-linked glycosylation interference	698:732	N-linked glycosylation interference	698:732	Since there are limited publications on 2-DG mechanism of action in breast cancer, we studied its effects in breast cancer cell lines to determine the part played by glycolysis inhibition and N-linked glycosylation interference.
29971627	1	20	theme	PURPOSE	151:157	arg1	cells					166:170	PURPOSE Cancer cells	151:170	PURPOSE Cancer cells	151:170	PURPOSE Cancer cells often elicit a higher glycolytic rate than normal cells, supporting the development of glycolysis inhibitors as therapeutic agents.
29971627	12	21	theme	glycolysis	1793:1802	arg1	disruption					1804:1813	glycolysis disruption	1793:1813	glycolysis disruption	1793:1813	CONCLUSION N-linked glycosylation interference and glycolysis disruption both contribute to the anticancer properties of 2-DG in breast cancer cells.
29971627	7	22	theme	incubated	1280:1288	arg1	cells					1301:1305	2-DG incubated MDA-MB-231 cells	1275:1305	2-DG incubated MDA-MB-231 cells	1275:1305	These results are reinforced by the appearance of an abnormal Man7GlcNAc2 structure both on lipid-linked oligosaccharides and N-linked glycoproteins of 2-DG incubated MDA-MB-231 cells.
29971627	5	23	theme	hormone-independent	891:909	arg1	cells					936:940	the hormone-dependent MCF-7 and hormone-independent MDA-MB-231 breast cancer cells	859:940	cells	936:940	METHODS AND RESULTS 2-Deoxyglucose behaved as an anticancer agent with a similar efficiency on cell number decrease between the hormone-dependent MCF-7 and hormone-independent MDA-MB-231 breast cancer cells.
29971627	0	24	theme	antiproliferative	84:100	arg1	action					102:107	the antiproliferative action	80:107	the antiproliferative action of 2-deoxyglucose in breast cancer cells	80:148	Protein N-glycosylation alteration and glycolysis inhibition both contribute to the antiproliferative action of 2-deoxyglucose in breast cancer cells.
29971627	5	25	theme	breast	922:927	arg1	cells					936:940	the hormone-dependent MCF-7 and hormone-independent MDA-MB-231 breast cancer cells	859:940	cells	936:940	METHODS AND RESULTS 2-Deoxyglucose behaved as an anticancer agent with a similar efficiency on cell number decrease between the hormone-dependent MCF-7 and hormone-independent MDA-MB-231 breast cancer cells.
29971627	12	26	theme	N-linked	1753:1760	arg1	interference					1776:1787	CONCLUSION N-linked glycosylation interference	1742:1787	CONCLUSION N-linked glycosylation interference	1742:1787	CONCLUSION N-linked glycosylation interference and glycolysis disruption both contribute to the anticancer properties of 2-DG in breast cancer cells.
29971627	10	27	theme	mannose	1486:1492	arg1	presence					1474:1481	the presence	1470:1481	the presence of mannose	1470:1492	2-DG, even in the presence of mannose, decreased glycolysis in both cell lines.
29971627	7	28	theme	N-linked	1249:1256	arg1	glycoproteins					1258:1270	N-linked glycoproteins	1249:1270	N-linked glycoproteins	1249:1270	These results are reinforced by the appearance of an abnormal Man7GlcNAc2 structure both on lipid-linked oligosaccharides and N-linked glycoproteins of 2-DG incubated MDA-MB-231 cells.
29971627	7	29	dep	appearance	1159:1168	arg1	both					1207:1210	both	1207:1210	both	1207:1210	These results are reinforced by the appearance of an abnormal Man7GlcNAc2 structure both on lipid-linked oligosaccharides and N-linked glycoproteins of 2-DG incubated MDA-MB-231 cells.
29971627	5	30	theme	METHODS	735:741	arg1	2-Deoxyglucose					755:768	METHODS AND RESULTS 2-Deoxyglucose	735:768	METHODS AND RESULTS 2-Deoxyglucose	735:768	METHODS AND RESULTS 2-Deoxyglucose behaved as an anticancer agent with a similar efficiency on cell number decrease between the hormone-dependent MCF-7 and hormone-independent MDA-MB-231 breast cancer cells.
29971627	12	31	from	properties	1849:1858	arg1	cells					1885:1889	breast cancer cells	1871:1889	breast cancer cells	1871:1889	CONCLUSION N-linked glycosylation interference and glycolysis disruption both contribute to the anticancer properties of 2-DG in breast cancer cells.
29971627	0	32	theme	Protein	0:6	arg1	alteration					24:33	Protein N-glycosylation alteration	0:33	Protein N-glycosylation alteration	0:33	Protein N-glycosylation alteration and glycolysis inhibition both contribute to the antiproliferative action of 2-deoxyglucose in breast cancer cells.
29971627	6	33	theme	membrane	1089:1096	arg1	protein					1098:1104	the lysosomal-associated membrane protein 2	1064:1106	the lysosomal-associated membrane protein 2	1064:1106	It also interfered with the N-linked glycosylation process in both cell lines as illustrated by the migration profile of the lysosomal-associated membrane protein 2 and calumenin.
29971627	11	34	from	cells	1721:1725	arg1	%					1664:1664	37 and 47%	1655:1664	37 and 47%	1655:1664	Mannose partially reversed the effects of 2-DG on cell numbers with N-linked glycosylation interference accounting for 37 and 47% of 2-DG anti-cancerous effects in MDA-MB-231 and MCF-7 cells, respectively.
29971627	11	34	from	cells	1721:1725	arg1	effects					1689:1695	2-DG anti-cancerous effects	1669:1695	2-DG anti-cancerous effects in MDA-MB-231 and MCF-7 cells	1669:1725	Mannose partially reversed the effects of 2-DG on cell numbers with N-linked glycosylation interference accounting for 37 and 47% of 2-DG anti-cancerous effects in MDA-MB-231 and MCF-7 cells, respectively.
29971627	7	35	theme	structure	1197:1205	arg1	appearance					1159:1168	the appearance	1155:1168	the appearance of an abnormal Man7GlcNAc2 structure	1155:1205	These results are reinforced by the appearance of an abnormal Man7GlcNAc2 structure both on lipid-linked oligosaccharides and N-linked glycoproteins of 2-DG incubated MDA-MB-231 cells.
29971627	3	36	theme	many	403:406	arg1	studies					408:414	many studies	403:414	many studies	403:414	However, many studies do not take into account that 2-DG inhibits not only glycolysis but also N-glycosylation.
29971627	7	37	theme	abnormal	1176:1183	arg1	structure					1197:1205	an abnormal Man7GlcNAc2 structure	1173:1205	an abnormal Man7GlcNAc2 structure	1173:1205	These results are reinforced by the appearance of an abnormal Man7GlcNAc2 structure both on lipid-linked oligosaccharides and N-linked glycoproteins of 2-DG incubated MDA-MB-231 cells.
29971627	0	38	theme	glycolysis	39:48	arg1	inhibition					50:59	glycolysis inhibition	39:59	glycolysis inhibition	39:59	Protein N-glycosylation alteration and glycolysis inhibition both contribute to the antiproliferative action of 2-deoxyglucose in breast cancer cells.
29971627	5	39	theme	RESULTS	747:753	arg1	2-Deoxyglucose					755:768	METHODS AND RESULTS 2-Deoxyglucose	735:768	METHODS AND RESULTS 2-Deoxyglucose	735:768	METHODS AND RESULTS 2-Deoxyglucose behaved as an anticancer agent with a similar efficiency on cell number decrease between the hormone-dependent MCF-7 and hormone-independent MDA-MB-231 breast cancer cells.
29971627	11	40	with	numbers	1591:1597	arg1	interference					1627:1638	N-linked glycosylation interference	1604:1638	N-linked glycosylation interference accounting for 37 and 47% of 2-DG anti-cancerous effects in MDA-MB-231 and MCF-7 cells, respectively	1604:1739	Mannose partially reversed the effects of 2-DG on cell numbers with N-linked glycosylation interference accounting for 37 and 47% of 2-DG anti-cancerous effects in MDA-MB-231 and MCF-7 cells, respectively.
29971627	5	41	dep	number	835:840	arg1	decrease					842:849	decrease	842:849	decrease	842:849	METHODS AND RESULTS 2-Deoxyglucose behaved as an anticancer agent with a similar efficiency on cell number decrease between the hormone-dependent MCF-7 and hormone-independent MDA-MB-231 breast cancer cells.
29971627	10	42	from	2-DG	1456:1459	arg1	presence					1474:1481	the presence	1470:1481	the presence of mannose	1470:1492	2-DG, even in the presence of mannose, decreased glycolysis in both cell lines.
29971627	11	43	from	%	1664:1664	arg1	cells					1721:1725	MDA-MB-231 and MCF-7 cells	1700:1725	cells	1721:1725	Mannose partially reversed the effects of 2-DG on cell numbers with N-linked glycosylation interference accounting for 37 and 47% of 2-DG anti-cancerous effects in MDA-MB-231 and MCF-7 cells, respectively.
29971627	12	44	theme	cancer	1878:1883	arg1	cells					1885:1889	breast cancer cells	1871:1889	breast cancer cells	1871:1889	CONCLUSION N-linked glycosylation interference and glycolysis disruption both contribute to the anticancer properties of 2-DG in breast cancer cells.
29971627	11	45	theme	MDA-MB-231	1700:1709	arg1	cells					1721:1725	MDA-MB-231 and MCF-7 cells	1700:1725	cells	1721:1725	Mannose partially reversed the effects of 2-DG on cell numbers with N-linked glycosylation interference accounting for 37 and 47% of 2-DG anti-cancerous effects in MDA-MB-231 and MCF-7 cells, respectively.
29971627	1	46	theme	glycolysis	259:268	arg1	inhibitors					270:279	glycolysis inhibitors	259:279	glycolysis inhibitors	259:279	PURPOSE Cancer cells often elicit a higher glycolytic rate than normal cells, supporting the development of glycolysis inhibitors as therapeutic agents.
29971627	12	47	theme	anticancer	1838:1847	arg1	properties					1849:1858	the anticancer properties	1834:1858	the anticancer properties of 2-DG in breast cancer cells	1834:1889	CONCLUSION N-linked glycosylation interference and glycolysis disruption both contribute to the anticancer properties of 2-DG in breast cancer cells.
29971627	7	48	theme	cells	1301:1305	arg1	oligosaccharides					1228:1243	lipid-linked oligosaccharides	1215:1243	lipid-linked oligosaccharides	1215:1243	These results are reinforced by the appearance of an abnormal Man7GlcNAc2 structure both on lipid-linked oligosaccharides and N-linked glycoproteins of 2-DG incubated MDA-MB-231 cells.
29971627	7	48	theme	cells	1301:1305	arg1	glycoproteins					1258:1270	N-linked glycoproteins	1249:1270	N-linked glycoproteins	1249:1270	These results are reinforced by the appearance of an abnormal Man7GlcNAc2 structure both on lipid-linked oligosaccharides and N-linked glycoproteins of 2-DG incubated MDA-MB-231 cells.
29971627	4	49	theme	action	564:569	arg1	mechanism					551:559	2-DG mechanism	546:559	2-DG mechanism of action	546:569	Since there are limited publications on 2-DG mechanism of action in breast cancer, we studied its effects in breast cancer cell lines to determine the part played by glycolysis inhibition and N-linked glycosylation interference.
29971627	11	50	theme	effects	1689:1695	arg1	%					1664:1664	37 and 47%	1655:1664	37 and 47%	1655:1664	Mannose partially reversed the effects of 2-DG on cell numbers with N-linked glycosylation interference accounting for 37 and 47% of 2-DG anti-cancerous effects in MDA-MB-231 and MCF-7 cells, respectively.
29971627	11	50	theme	effects	1689:1695	arg1	effects					1689:1695	2-DG anti-cancerous effects	1669:1695	2-DG anti-cancerous effects in MDA-MB-231 and MCF-7 cells	1669:1725	Mannose partially reversed the effects of 2-DG on cell numbers with N-linked glycosylation interference accounting for 37 and 47% of 2-DG anti-cancerous effects in MDA-MB-231 and MCF-7 cells, respectively.
29971627	7	51	link	lipid-linked	1215:1226	arg1	oligosaccharides					1228:1243	lipid-linked oligosaccharides	1215:1243	lipid-linked oligosaccharides	1215:1243	These results are reinforced by the appearance of an abnormal Man7GlcNAc2 structure both on lipid-linked oligosaccharides and N-linked glycoproteins of 2-DG incubated MDA-MB-231 cells.
29971627	5	52	with	agent	795:799	arg1	efficiency					816:825	a similar efficiency	806:825	a similar efficiency on cell number decrease between the hormone-dependent MCF-7 and hormone-independent MDA-MB-231 breast cancer cells	806:940	METHODS AND RESULTS 2-Deoxyglucose behaved as an anticancer agent with a similar efficiency on cell number decrease between the hormone-dependent MCF-7 and hormone-independent MDA-MB-231 breast cancer cells.
29971627	4	53	theme	breast	574:579	arg1	cancer					581:586	breast cancer	574:586	breast cancer	574:586	Since there are limited publications on 2-DG mechanism of action in breast cancer, we studied its effects in breast cancer cell lines to determine the part played by glycolysis inhibition and N-linked glycosylation interference.
29971627	8	54	theme	endoplasmic	1342:1352	arg1	reticulum					1354:1362	a transient endoplasmic reticulum	1330:1362	2-DG-induced a transient endoplasmic reticulum stress that was more sustained in MDA-MB-231 cells	1317:1413	Besides, 2-DG-induced a transient endoplasmic reticulum stress that was more sustained in MDA-MB-231 cells.
29971627	1	55	theme	glycolytic	194:203	arg1	rate					205:208	a higher glycolytic rate	185:208	a higher glycolytic rate than normal cells	185:226	PURPOSE Cancer cells often elicit a higher glycolytic rate than normal cells, supporting the development of glycolysis inhibitors as therapeutic agents.
29971627	4	56	theme	cell	629:632	arg1	lines					634:638	breast cancer cell lines	615:638	breast cancer cell lines	615:638	Since there are limited publications on 2-DG mechanism of action in breast cancer, we studied its effects in breast cancer cell lines to determine the part played by glycolysis inhibition and N-linked glycosylation interference.
29971627	11	57	theme	MCF-7	1715:1719	arg1	cells					1721:1725	MDA-MB-231 and MCF-7 cells	1700:1725	cells	1721:1725	Mannose partially reversed the effects of 2-DG on cell numbers with N-linked glycosylation interference accounting for 37 and 47% of 2-DG anti-cancerous effects in MDA-MB-231 and MCF-7 cells, respectively.
29971627	5	58	theme	hormone-dependent	863:879	arg1	MCF-7					881:885	the hormone-dependent MCF-7 and hormone-independent MDA-MB-231 breast cancer cells	859:940	MCF-7	881:885	METHODS AND RESULTS 2-Deoxyglucose behaved as an anticancer agent with a similar efficiency on cell number decrease between the hormone-dependent MCF-7 and hormone-independent MDA-MB-231 breast cancer cells.
29971627	11	59	theme	N-linked	1604:1611	arg1	interference					1627:1638	N-linked glycosylation interference	1604:1638	N-linked glycosylation interference accounting for 37 and 47% of 2-DG anti-cancerous effects in MDA-MB-231 and MCF-7 cells, respectively	1604:1739	Mannose partially reversed the effects of 2-DG on cell numbers with N-linked glycosylation interference accounting for 37 and 47% of 2-DG anti-cancerous effects in MDA-MB-231 and MCF-7 cells, respectively.
29971627	6	60	theme	glycosylation	980:992	arg1	process					994:1000	the N-linked glycosylation process	967:1000	the N-linked glycosylation process in both cell lines	967:1019	It also interfered with the N-linked glycosylation process in both cell lines as illustrated by the migration profile of the lysosomal-associated membrane protein 2 and calumenin.
29971627	0	61	from	action	102:107	arg1	cells					144:148	breast cancer cells	130:148	breast cancer cells	130:148	Protein N-glycosylation alteration and glycolysis inhibition both contribute to the antiproliferative action of 2-deoxyglucose in breast cancer cells.
29971627	4	62	from	effects	604:610	arg1	lines					634:638	breast cancer cell lines	615:638	breast cancer cell lines	615:638	Since there are limited publications on 2-DG mechanism of action in breast cancer, we studied its effects in breast cancer cell lines to determine the part played by glycolysis inhibition and N-linked glycosylation interference.
29971627	4	63	theme	breast	615:620	arg1	lines					634:638	breast cancer cell lines	615:638	breast cancer cell lines	615:638	Since there are limited publications on 2-DG mechanism of action in breast cancer, we studied its effects in breast cancer cell lines to determine the part played by glycolysis inhibition and N-linked glycosylation interference.
29971627	12	64	link	N-linked	1753:1760	arg1	interference					1776:1787	CONCLUSION N-linked glycosylation interference	1742:1787	CONCLUSION N-linked glycosylation interference	1742:1787	CONCLUSION N-linked glycosylation interference and glycolysis disruption both contribute to the anticancer properties of 2-DG in breast cancer cells.
29971627	12	65	from	2-DG	1863:1866	arg1	cells					1885:1889	breast cancer cells	1871:1889	breast cancer cells	1871:1889	CONCLUSION N-linked glycosylation interference and glycolysis disruption both contribute to the anticancer properties of 2-DG in breast cancer cells.
29971627	5	66	theme	cancer	929:934	arg1	cells					936:940	the hormone-dependent MCF-7 and hormone-independent MDA-MB-231 breast cancer cells	859:940	cells	936:940	METHODS AND RESULTS 2-Deoxyglucose behaved as an anticancer agent with a similar efficiency on cell number decrease between the hormone-dependent MCF-7 and hormone-independent MDA-MB-231 breast cancer cells.
29971627	4	67	theme	glycolysis	672:681	arg1	inhibition					683:692	glycolysis inhibition	672:692	glycolysis inhibition	672:692	Since there are limited publications on 2-DG mechanism of action in breast cancer, we studied its effects in breast cancer cell lines to determine the part played by glycolysis inhibition and N-linked glycosylation interference.
29971627	7	68	theme	Man7GlcNAc2	1185:1195	arg1	structure					1197:1205	an abnormal Man7GlcNAc2 structure	1173:1205	an abnormal Man7GlcNAc2 structure	1173:1205	These results are reinforced by the appearance of an abnormal Man7GlcNAc2 structure both on lipid-linked oligosaccharides and N-linked glycoproteins of 2-DG incubated MDA-MB-231 cells.
29971627	12	69	theme	glycosylation	1762:1774	arg1	interference					1776:1787	CONCLUSION N-linked glycosylation interference	1742:1787	CONCLUSION N-linked glycosylation interference	1742:1787	CONCLUSION N-linked glycosylation interference and glycolysis disruption both contribute to the anticancer properties of 2-DG in breast cancer cells.
29971627	6	70	theme	migration	1043:1051	arg1	profile					1053:1059	the migration profile	1039:1059	the migration profile of the lysosomal-associated membrane protein 2 and calumenin	1039:1120	It also interfered with the N-linked glycosylation process in both cell lines as illustrated by the migration profile of the lysosomal-associated membrane protein 2 and calumenin.
29971627	10	71	theme	cell	1524:1527	arg1	lines					1529:1533	both cell lines	1519:1533	both cell lines	1519:1533	2-DG, even in the presence of mannose, decreased glycolysis in both cell lines.
29971627	1	72	theme	Cancer	159:164	arg1	cells					166:170	PURPOSE Cancer cells	151:170	PURPOSE Cancer cells	151:170	PURPOSE Cancer cells often elicit a higher glycolytic rate than normal cells, supporting the development of glycolysis inhibitors as therapeutic agents.
29971627	5	73	theme	MDA-MB-231	911:920	arg1	cells					936:940	the hormone-dependent MCF-7 and hormone-independent MDA-MB-231 breast cancer cells	859:940	cells	936:940	METHODS AND RESULTS 2-Deoxyglucose behaved as an anticancer agent with a similar efficiency on cell number decrease between the hormone-dependent MCF-7 and hormone-independent MDA-MB-231 breast cancer cells.
29971627	0	74	theme	2-deoxyglucose	112:125	arg1	action					102:107	the antiproliferative action	80:107	the antiproliferative action of 2-deoxyglucose in breast cancer cells	80:148	Protein N-glycosylation alteration and glycolysis inhibition both contribute to the antiproliferative action of 2-deoxyglucose in breast cancer cells.
29971627	12	75	theme	CONCLUSION	1742:1751	arg1	interference					1776:1787	CONCLUSION N-linked glycosylation interference	1742:1787	CONCLUSION N-linked glycosylation interference	1742:1787	CONCLUSION N-linked glycosylation interference and glycolysis disruption both contribute to the anticancer properties of 2-DG in breast cancer cells.
29971627	6	76	theme	cell	1010:1013	arg1	lines					1015:1019	both cell lines	1005:1019	both cell lines	1005:1019	It also interfered with the N-linked glycosylation process in both cell lines as illustrated by the migration profile of the lysosomal-associated membrane protein 2 and calumenin.
29971627	8	77	theme	MDA-MB-231	1398:1407	arg1	cells					1409:1413	MDA-MB-231 cells	1398:1413	MDA-MB-231 cells	1398:1413	Besides, 2-DG-induced a transient endoplasmic reticulum stress that was more sustained in MDA-MB-231 cells.
29971627	12	78	from	cells	1885:1889	arg1	properties					1849:1858	the anticancer properties	1834:1858	the anticancer properties of 2-DG in breast cancer cells	1834:1889	CONCLUSION N-linked glycosylation interference and glycolysis disruption both contribute to the anticancer properties of 2-DG in breast cancer cells.
29971627	0	79	theme	breast	130:135	arg1	cells					144:148	breast cancer cells	130:148	breast cancer cells	130:148	Protein N-glycosylation alteration and glycolysis inhibition both contribute to the antiproliferative action of 2-deoxyglucose in breast cancer cells.
29971627	5	80	from	efficiency	816:825	arg1	number					835:840	cell number decrease	830:849	cell number decrease	830:849	METHODS AND RESULTS 2-Deoxyglucose behaved as an anticancer agent with a similar efficiency on cell number decrease between the hormone-dependent MCF-7 and hormone-independent MDA-MB-231 breast cancer cells.
29971627	1	81	theme	normal	215:220	arg1	cells					222:226	normal cells	215:226	normal cells	215:226	PURPOSE Cancer cells often elicit a higher glycolytic rate than normal cells, supporting the development of glycolysis inhibitors as therapeutic agents.
29971627	11	82	from	effects	1567:1573	arg1	numbers					1591:1597	cell numbers	1586:1597	cell numbers with N-linked glycosylation interference accounting for 37 and 47% of 2-DG anti-cancerous effects in MDA-MB-231 and MCF-7 cells, respectively	1586:1739	Mannose partially reversed the effects of 2-DG on cell numbers with N-linked glycosylation interference accounting for 37 and 47% of 2-DG anti-cancerous effects in MDA-MB-231 and MCF-7 cells, respectively.
29971627	7	83	theme	lipid-linked	1215:1226	arg1	oligosaccharides					1228:1243	lipid-linked oligosaccharides	1215:1243	lipid-linked oligosaccharides	1215:1243	These results are reinforced by the appearance of an abnormal Man7GlcNAc2 structure both on lipid-linked oligosaccharides and N-linked glycoproteins of 2-DG incubated MDA-MB-231 cells.
29971627	0	84	theme	N-glycosylation	8:22	arg1	alteration					24:33	Protein N-glycosylation alteration	0:33	Protein N-glycosylation alteration	0:33	Protein N-glycosylation alteration and glycolysis inhibition both contribute to the antiproliferative action of 2-deoxyglucose in breast cancer cells.
29971627	8	85	from	sustained	1385:1393	arg1	cells					1409:1413	MDA-MB-231 cells	1398:1413	MDA-MB-231 cells	1398:1413	Besides, 2-DG-induced a transient endoplasmic reticulum stress that was more sustained in MDA-MB-231 cells.
29971627	6	86	theme	protein	1098:1104	arg1	profile					1053:1059	the migration profile	1039:1059	the migration profile of the lysosomal-associated membrane protein 2 and calumenin	1039:1120	It also interfered with the N-linked glycosylation process in both cell lines as illustrated by the migration profile of the lysosomal-associated membrane protein 2 and calumenin.
29971627	4	87	theme	glycosylation	707:719	arg1	interference					721:732	N-linked glycosylation interference	698:732	N-linked glycosylation interference	698:732	Since there are limited publications on 2-DG mechanism of action in breast cancer, we studied its effects in breast cancer cell lines to determine the part played by glycolysis inhibition and N-linked glycosylation interference.
29971627	5	88	theme	similar	808:814	arg1	efficiency					816:825	a similar efficiency	806:825	a similar efficiency on cell number decrease between the hormone-dependent MCF-7 and hormone-independent MDA-MB-231 breast cancer cells	806:940	METHODS AND RESULTS 2-Deoxyglucose behaved as an anticancer agent with a similar efficiency on cell number decrease between the hormone-dependent MCF-7 and hormone-independent MDA-MB-231 breast cancer cells.
29971627	6	89	theme	lysosomal-associated	1068:1087	arg1	protein					1098:1104	the lysosomal-associated membrane protein 2	1064:1106	the lysosomal-associated membrane protein 2	1064:1106	It also interfered with the N-linked glycosylation process in both cell lines as illustrated by the migration profile of the lysosomal-associated membrane protein 2 and calumenin.
29971627	7	90	gly	glycoproteins	1258:1270	arg1	glycoproteins					1258:1270	N-linked glycoproteins	1249:1270	N-linked glycoproteins	1249:1270	These results are reinforced by the appearance of an abnormal Man7GlcNAc2 structure both on lipid-linked oligosaccharides and N-linked glycoproteins of 2-DG incubated MDA-MB-231 cells.
29971627	4	91	from	publications	530:541	arg1	cancer					581:586	breast cancer	574:586	breast cancer	574:586	Since there are limited publications on 2-DG mechanism of action in breast cancer, we studied its effects in breast cancer cell lines to determine the part played by glycolysis inhibition and N-linked glycosylation interference.
29971627	4	91	from	publications	530:541	arg1	mechanism					551:559	2-DG mechanism	546:559	2-DG mechanism of action	546:569	Since there are limited publications on 2-DG mechanism of action in breast cancer, we studied its effects in breast cancer cell lines to determine the part played by glycolysis inhibition and N-linked glycosylation interference.
29971627	4	92	theme	cancer	622:627	arg1	lines					634:638	breast cancer cell lines	615:638	breast cancer cell lines	615:638	Since there are limited publications on 2-DG mechanism of action in breast cancer, we studied its effects in breast cancer cell lines to determine the part played by glycolysis inhibition and N-linked glycosylation interference.
29971627	11	93	from	effects	1689:1695	arg1	cells					1721:1725	MDA-MB-231 and MCF-7 cells	1700:1725	cells	1721:1725	Mannose partially reversed the effects of 2-DG on cell numbers with N-linked glycosylation interference accounting for 37 and 47% of 2-DG anti-cancerous effects in MDA-MB-231 and MCF-7 cells, respectively.
29971627	6	94	from	process	994:1000	arg1	lines					1015:1019	both cell lines	1005:1019	both cell lines	1005:1019	It also interfered with the N-linked glycosylation process in both cell lines as illustrated by the migration profile of the lysosomal-associated membrane protein 2 and calumenin.
29971627	5	95	theme	anticancer	784:793	arg1	agent					795:799	an anticancer agent	781:799	an anticancer agent with a similar efficiency on cell number decrease between the hormone-dependent MCF-7 and hormone-independent MDA-MB-231 breast cancer cells	781:940	METHODS AND RESULTS 2-Deoxyglucose behaved as an anticancer agent with a similar efficiency on cell number decrease between the hormone-dependent MCF-7 and hormone-independent MDA-MB-231 breast cancer cells.
29971627	8	96	from	cells	1409:1413	arg1	sustained					1385:1393	sustained	1385:1393	sustained	1385:1393	Besides, 2-DG-induced a transient endoplasmic reticulum stress that was more sustained in MDA-MB-231 cells.
29971627	11	97	link	N-linked	1604:1611	arg1	interference					1627:1638	N-linked glycosylation interference	1604:1638	N-linked glycosylation interference accounting for 37 and 47% of 2-DG anti-cancerous effects in MDA-MB-231 and MCF-7 cells, respectively	1604:1739	Mannose partially reversed the effects of 2-DG on cell numbers with N-linked glycosylation interference accounting for 37 and 47% of 2-DG anti-cancerous effects in MDA-MB-231 and MCF-7 cells, respectively.
29971627	12	98	theme	2-DG	1863:1866	arg1	properties					1849:1858	the anticancer properties	1834:1858	the anticancer properties of 2-DG in breast cancer cells	1834:1889	CONCLUSION N-linked glycosylation interference and glycolysis disruption both contribute to the anticancer properties of 2-DG in breast cancer cells.
29971627	2	99	used	used	329:332	arg2	2-DG					320:323	2-DG	320:323	2-DG	320:323	2-Deoxyglucose (2-DG) is used in this context due to its ability to compete with glucose.
29971627	2	99	used	used	329:332	arg2	2-Deoxyglucose					304:317	2-Deoxyglucose	304:317	2-Deoxyglucose (2-DG)	304:324	2-Deoxyglucose (2-DG) is used in this context due to its ability to compete with glucose.
30248972	6	0	theme	geographical	1488:1499	arg1	region					1501:1506	the geographical region	1484:1506	the geographical region	1484:1506	Additionally, the effect of caesarean section on milk metabolome was dependent on the geographical region.
30248972	1	1	theme	breast	152:157	arg1	milk					159:162	human breast milk	146:162	human breast milk	146:162	The composition of human breast milk is highly variable, and it can be influenced by genetics, diet, lifestyle, and other environmental factors.
30248972	5	2	theme	N-acetyl	1161:1168	arg1	lysine					1170:1175	N-acetyl lysine	1161:1175	N-acetyl lysine	1161:1175	The metabolite profiles showed significant differences between geographical locations, with significant differences (p < 0.05) in the levels of galactose, lacto-N-fucopentaose III, lacto-N-fucopentaose I and 2-fucosyllactose, 3-fucosyllactose, lacto-N-difucohexaose II, lacto-N-fucopentaose III, 2-hydroxybutyrate, 3-hydroxybutyrate, proline, N-acetyl lysine, methyl-histidine, dimethylamine, kynurenine, urea, creatine and creatine phosphate, formate, lactate, acetate, phosphocholine, acetylcholine, LDL, VLDL, ethanolamine, riboflavin, hippurate, spermidine, spermine and uridine.
30248972	2	3	theme	milk	442:445	arg1	profile					424:430	the nuclear magnetic resonance spectroscopy (NMR) metabolic profile	364:430	the nuclear magnetic resonance spectroscopy (NMR) metabolic profile of breast milk	364:445	This study aimed to investigate the impact of geographical location and mode of delivery on the nuclear magnetic resonance spectroscopy (NMR) metabolic profile of breast milk and its relationship with the milk microbiome.
30248972	2	3	theme	milk	442:445	arg1	relationship					455:466	its relationship	451:466	its relationship with the milk microbiome	451:491	This study aimed to investigate the impact of geographical location and mode of delivery on the nuclear magnetic resonance spectroscopy (NMR) metabolic profile of breast milk and its relationship with the milk microbiome.
30248972	2	4	with	relationship	455:466	arg1	microbiome					482:491	the milk microbiome	473:491	the milk microbiome	473:491	This study aimed to investigate the impact of geographical location and mode of delivery on the nuclear magnetic resonance spectroscopy (NMR) metabolic profile of breast milk and its relationship with the milk microbiome.
30248972	1	5	theme	milk	159:162	arg1	variable					174:181	variable	174:181	variable	174:181	The composition of human breast milk is highly variable, and it can be influenced by genetics, diet, lifestyle, and other environmental factors.
30248972	1	5	theme	milk	159:162	arg1	composition					131:141	The composition	127:141	The composition of human breast milk	127:162	The composition of human breast milk is highly variable, and it can be influenced by genetics, diet, lifestyle, and other environmental factors.
30248972	7	6	theme	Specific	1509:1516	arg1	interrelations					1518:1531	Specific interrelations	1509:1531	Specific interrelations between human milk metabolites and microbiota	1509:1577	Specific interrelations between human milk metabolites and microbiota were also identified.
30248972	5	7	from	I	1020:1020	arg1	levels					952:957	the levels	948:957	the levels of galactose	948:970	The metabolite profiles showed significant differences between geographical locations, with significant differences (p < 0.05) in the levels of galactose, lacto-N-fucopentaose III, lacto-N-fucopentaose I and 2-fucosyllactose, 3-fucosyllactose, lacto-N-difucohexaose II, lacto-N-fucopentaose III, 2-hydroxybutyrate, 3-hydroxybutyrate, proline, N-acetyl lysine, methyl-histidine, dimethylamine, kynurenine, urea, creatine and creatine phosphate, formate, lactate, acetate, phosphocholine, acetylcholine, LDL, VLDL, ethanolamine, riboflavin, hippurate, spermidine, spermine and uridine.
30248972	4	8	theme	NMR	805:807	arg1	spectra					809:815	the milk NMR spectra	796:815	the milk NMR spectra	796:815	Up to 68 metabolites, including amino acids, oligosaccharides, and fatty acid-associated metabolites, were identified in the milk NMR spectra.
30248972	2	9	from	impact	308:313	arg1	profile					424:430	the nuclear magnetic resonance spectroscopy (NMR) metabolic profile	364:430	the nuclear magnetic resonance spectroscopy (NMR) metabolic profile of breast milk	364:445	This study aimed to investigate the impact of geographical location and mode of delivery on the nuclear magnetic resonance spectroscopy (NMR) metabolic profile of breast milk and its relationship with the milk microbiome.
30248972	2	9	from	impact	308:313	arg1	relationship					455:466	its relationship	451:466	its relationship with the milk microbiome	451:491	This study aimed to investigate the impact of geographical location and mode of delivery on the nuclear magnetic resonance spectroscopy (NMR) metabolic profile of breast milk and its relationship with the milk microbiome.
30248972	9	10	theme	potential	1892:1900	arg1	interactions					1902:1913	the potential interactions	1888:1913	the potential interactions between human milk's metabolites and microbes	1888:1959	Our results reveal specific milk metabolomic profiles across geographical locations and also highlight the potential interactions between human milk's metabolites and microbes.
30248972	2	11	theme	milk	477:480	arg1	microbiome					482:491	the milk microbiome	473:491	the milk microbiome	473:491	This study aimed to investigate the impact of geographical location and mode of delivery on the nuclear magnetic resonance spectroscopy (NMR) metabolic profile of breast milk and its relationship with the milk microbiome.
30248972	4	12	theme	milk	800:803	arg1	spectra					809:815	the milk NMR spectra	796:815	the milk NMR spectra	796:815	Up to 68 metabolites, including amino acids, oligosaccharides, and fatty acid-associated metabolites, were identified in the milk NMR spectra.
30248972	0	13	with	Locations	71:79	arg1	Microbiota					115:124	the Milk Microbiota	106:124	the Milk Microbiota	106:124	Human Breast Milk NMR Metabolomic Profile across Specific Geographical Locations and Its Association with the Milk Microbiota.
30248972	2	14	theme	geographical	318:329	arg1	location					331:338	geographical location	318:338	geographical location	318:338	This study aimed to investigate the impact of geographical location and mode of delivery on the nuclear magnetic resonance spectroscopy (NMR) metabolic profile of breast milk and its relationship with the milk microbiome.
30248972	0	15	theme	Milk	110:113	arg1	Microbiota					115:124	the Milk Microbiota	106:124	the Milk Microbiota	106:124	Human Breast Milk NMR Metabolomic Profile across Specific Geographical Locations and Its Association with the Milk Microbiota.
30248972	3	16	from	China	668:672	arg1	women					623:627	79 healthy women	612:627	79 healthy women from Finland, Spain, South Africa, and China	612:672	Human milk metabolic and microbiota profiles were determined using NMR and 16S rRNA gene sequencing, respectively, in 79 healthy women from Finland, Spain, South Africa, and China.
30248972	9	17	theme	milk	1813:1816	arg1	profiles					1830:1837	specific milk metabolomic profiles	1804:1837	specific milk metabolomic profiles across geographical locations	1804:1867	Our results reveal specific milk metabolomic profiles across geographical locations and also highlight the potential interactions between human milk's metabolites and microbes.
30248972	5	18	theme	metabolite	822:831	arg1	profiles					833:840	The metabolite profiles	818:840	The metabolite profiles	818:840	The metabolite profiles showed significant differences between geographical locations, with significant differences (p < 0.05) in the levels of galactose, lacto-N-fucopentaose III, lacto-N-fucopentaose I and 2-fucosyllactose, 3-fucosyllactose, lacto-N-difucohexaose II, lacto-N-fucopentaose III, 2-hydroxybutyrate, 3-hydroxybutyrate, proline, N-acetyl lysine, methyl-histidine, dimethylamine, kynurenine, urea, creatine and creatine phosphate, formate, lactate, acetate, phosphocholine, acetylcholine, LDL, VLDL, ethanolamine, riboflavin, hippurate, spermidine, spermine and uridine.
30248972	9	19	theme	specific	1804:1811	arg1	profiles					1830:1837	specific milk metabolomic profiles	1804:1837	specific milk metabolomic profiles across geographical locations	1804:1867	Our results reveal specific milk metabolomic profiles across geographical locations and also highlight the potential interactions between human milk's metabolites and microbes.
30248972	3	20	from	Africa	656:661	arg1	women					623:627	79 healthy women	612:627	79 healthy women from Finland, Spain, South Africa, and China	612:672	Human milk metabolic and microbiota profiles were determined using NMR and 16S rRNA gene sequencing, respectively, in 79 healthy women from Finland, Spain, South Africa, and China.
30248972	8	21	theme	milk	1689:1692	arg1	metabolites					1694:1704	the milk metabolites	1685:1704	the milk metabolites	1685:1704	Proteobacteria, Actinobacteria, and Bacilli were most significantly associated with the milk metabolites, being either positively or negatively correlated depending on the metabolite.
30248972	2	22	theme	delivery	352:359	arg1	mode					344:347	mode	344:347	mode of delivery	344:359	This study aimed to investigate the impact of geographical location and mode of delivery on the nuclear magnetic resonance spectroscopy (NMR) metabolic profile of breast milk and its relationship with the milk microbiome.
30248972	2	22	theme	delivery	352:359	arg1	location					331:338	geographical location	318:338	geographical location	318:338	This study aimed to investigate the impact of geographical location and mode of delivery on the nuclear magnetic resonance spectroscopy (NMR) metabolic profile of breast milk and its relationship with the milk microbiome.
30248972	0	23	theme	Breast	6:11	arg1	Milk					13:16	Human Breast Milk	0:16	Human Breast Milk	0:16	Human Breast Milk NMR Metabolomic Profile across Specific Geographical Locations and Its Association with the Milk Microbiota.
30248972	0	24	theme	Human	0:4	arg1	Milk					13:16	Human Breast Milk	0:16	Human Breast Milk	0:16	Human Breast Milk NMR Metabolomic Profile across Specific Geographical Locations and Its Association with the Milk Microbiota.
30248972	5	25	theme	geographical	881:892	arg1	locations					894:902	geographical locations	881:902	geographical locations	881:902	The metabolite profiles showed significant differences between geographical locations, with significant differences (p < 0.05) in the levels of galactose, lacto-N-fucopentaose III, lacto-N-fucopentaose I and 2-fucosyllactose, 3-fucosyllactose, lacto-N-difucohexaose II, lacto-N-fucopentaose III, 2-hydroxybutyrate, 3-hydroxybutyrate, proline, N-acetyl lysine, methyl-histidine, dimethylamine, kynurenine, urea, creatine and creatine phosphate, formate, lactate, acetate, phosphocholine, acetylcholine, LDL, VLDL, ethanolamine, riboflavin, hippurate, spermidine, spermine and uridine.
30248972	2	26	theme	mode	344:347	arg1	impact					308:313	the impact	304:313	the impact of geographical location and mode of delivery on the nuclear magnetic resonance spectroscopy (NMR) metabolic profile of breast milk and its relationship with the milk microbiome	304:491	This study aimed to investigate the impact of geographical location and mode of delivery on the nuclear magnetic resonance spectroscopy (NMR) metabolic profile of breast milk and its relationship with the milk microbiome.
30248972	5	27	from	2-fucosyllactose	1026:1041	arg1	levels					952:957	the levels	948:957	the levels of galactose	948:970	The metabolite profiles showed significant differences between geographical locations, with significant differences (p < 0.05) in the levels of galactose, lacto-N-fucopentaose III, lacto-N-fucopentaose I and 2-fucosyllactose, 3-fucosyllactose, lacto-N-difucohexaose II, lacto-N-fucopentaose III, 2-hydroxybutyrate, 3-hydroxybutyrate, proline, N-acetyl lysine, methyl-histidine, dimethylamine, kynurenine, urea, creatine and creatine phosphate, formate, lactate, acetate, phosphocholine, acetylcholine, LDL, VLDL, ethanolamine, riboflavin, hippurate, spermidine, spermine and uridine.
30248972	1	28	theme	other	243:247	arg1	factors					263:269	other environmental factors	243:269	other environmental factors	243:269	The composition of human breast milk is highly variable, and it can be influenced by genetics, diet, lifestyle, and other environmental factors.
30248972	0	29	with	Association	89:99	arg1	Microbiota					115:124	the Milk Microbiota	106:124	the Milk Microbiota	106:124	Human Breast Milk NMR Metabolomic Profile across Specific Geographical Locations and Its Association with the Milk Microbiota.
30248972	6	30	theme	milk	1451:1454	arg1	metabolome					1456:1465	milk metabolome	1451:1465	milk metabolome	1451:1465	Additionally, the effect of caesarean section on milk metabolome was dependent on the geographical region.
30248972	2	31	theme	resonance	385:393	arg1	NMR					409:411	NMR	409:411	NMR	409:411	This study aimed to investigate the impact of geographical location and mode of delivery on the nuclear magnetic resonance spectroscopy (NMR) metabolic profile of breast milk and its relationship with the milk microbiome.
30248972	2	31	theme	resonance	385:393	arg1	spectroscopy					395:406	the nuclear magnetic resonance spectroscopy	364:406	the nuclear magnetic resonance spectroscopy (NMR) metabolic profile of breast milk	364:445	This study aimed to investigate the impact of geographical location and mode of delivery on the nuclear magnetic resonance spectroscopy (NMR) metabolic profile of breast milk and its relationship with the milk microbiome.
30248972	2	32	theme	spectroscopy	395:406	arg1	profile					424:430	the nuclear magnetic resonance spectroscopy (NMR) metabolic profile	364:430	the nuclear magnetic resonance spectroscopy (NMR) metabolic profile of breast milk	364:445	This study aimed to investigate the impact of geographical location and mode of delivery on the nuclear magnetic resonance spectroscopy (NMR) metabolic profile of breast milk and its relationship with the milk microbiome.
30248972	1	33	theme	environmental	249:261	arg1	factors					263:269	other environmental factors	243:269	other environmental factors	243:269	The composition of human breast milk is highly variable, and it can be influenced by genetics, diet, lifestyle, and other environmental factors.
30248972	3	34	theme	microbiota	519:528	arg1	profiles					530:537	Human milk metabolic and microbiota profiles	494:537	profiles	530:537	Human milk metabolic and microbiota profiles were determined using NMR and 16S rRNA gene sequencing, respectively, in 79 healthy women from Finland, Spain, South Africa, and China.
30248972	9	35	theme	human	1923:1927	arg1	metabolites					1936:1946	human milk's metabolites	1923:1946	human milk's metabolites	1923:1946	Our results reveal specific milk metabolomic profiles across geographical locations and also highlight the potential interactions between human milk's metabolites and microbes.
30248972	2	36	theme	magnetic	376:383	arg1	resonance					385:393	the nuclear magnetic resonance	364:393	the nuclear magnetic resonance spectroscopy (NMR) metabolic profile of breast milk	364:445	This study aimed to investigate the impact of geographical location and mode of delivery on the nuclear magnetic resonance spectroscopy (NMR) metabolic profile of breast milk and its relationship with the milk microbiome.
30248972	2	37	theme	breast	435:440	arg1	milk					442:445	breast milk	435:445	breast milk	435:445	This study aimed to investigate the impact of geographical location and mode of delivery on the nuclear magnetic resonance spectroscopy (NMR) metabolic profile of breast milk and its relationship with the milk microbiome.
30248972	5	38	from	lacto-N-difucohexaose	1062:1082	arg1	levels					952:957	the levels	948:957	the levels of galactose	948:970	The metabolite profiles showed significant differences between geographical locations, with significant differences (p < 0.05) in the levels of galactose, lacto-N-fucopentaose III, lacto-N-fucopentaose I and 2-fucosyllactose, 3-fucosyllactose, lacto-N-difucohexaose II, lacto-N-fucopentaose III, 2-hydroxybutyrate, 3-hydroxybutyrate, proline, N-acetyl lysine, methyl-histidine, dimethylamine, kynurenine, urea, creatine and creatine phosphate, formate, lactate, acetate, phosphocholine, acetylcholine, LDL, VLDL, ethanolamine, riboflavin, hippurate, spermidine, spermine and uridine.
30248972	5	39	theme	lacto-N-fucopentaose	999:1018	arg1	I					1020:1020	lacto-N-fucopentaose I	999:1020	lacto-N-fucopentaose I	999:1020	The metabolite profiles showed significant differences between geographical locations, with significant differences (p < 0.05) in the levels of galactose, lacto-N-fucopentaose III, lacto-N-fucopentaose I and 2-fucosyllactose, 3-fucosyllactose, lacto-N-difucohexaose II, lacto-N-fucopentaose III, 2-hydroxybutyrate, 3-hydroxybutyrate, proline, N-acetyl lysine, methyl-histidine, dimethylamine, kynurenine, urea, creatine and creatine phosphate, formate, lactate, acetate, phosphocholine, acetylcholine, LDL, VLDL, ethanolamine, riboflavin, hippurate, spermidine, spermine and uridine.
30248972	6	40	from	effect	1420:1425	arg1	metabolome					1456:1465	milk metabolome	1451:1465	milk metabolome	1451:1465	Additionally, the effect of caesarean section on milk metabolome was dependent on the geographical region.
30248972	6	41	theme	section	1440:1446	arg1	effect					1420:1425	the effect	1416:1425	the effect of caesarean section on milk metabolome	1416:1465	Additionally, the effect of caesarean section on milk metabolome was dependent on the geographical region.
30248972	6	41	theme	section	1440:1446	arg1	dependent					1471:1479	dependent	1471:1479	dependent	1471:1479	Additionally, the effect of caesarean section on milk metabolome was dependent on the geographical region.
30248972	2	42	theme	nuclear	368:374	arg1	resonance					385:393	the nuclear magnetic resonance	364:393	the nuclear magnetic resonance spectroscopy (NMR) metabolic profile of breast milk	364:445	This study aimed to investigate the impact of geographical location and mode of delivery on the nuclear magnetic resonance spectroscopy (NMR) metabolic profile of breast milk and its relationship with the milk microbiome.
30248972	0	43	theme	Metabolomic	22:32	arg1	Profile					34:40	Metabolomic Profile	22:40	Metabolomic Profile	22:40	Human Breast Milk NMR Metabolomic Profile across Specific Geographical Locations and Its Association with the Milk Microbiota.
30248972	5	44	from	lacto-N-fucopentaose	1088:1107	arg1	levels					952:957	the levels	948:957	the levels of galactose	948:970	The metabolite profiles showed significant differences between geographical locations, with significant differences (p < 0.05) in the levels of galactose, lacto-N-fucopentaose III, lacto-N-fucopentaose I and 2-fucosyllactose, 3-fucosyllactose, lacto-N-difucohexaose II, lacto-N-fucopentaose III, 2-hydroxybutyrate, 3-hydroxybutyrate, proline, N-acetyl lysine, methyl-histidine, dimethylamine, kynurenine, urea, creatine and creatine phosphate, formate, lactate, acetate, phosphocholine, acetylcholine, LDL, VLDL, ethanolamine, riboflavin, hippurate, spermidine, spermine and uridine.
30248972	0	45	theme	Specific	49:56	arg1	Locations					71:79	Specific Geographical Locations	49:79	Specific Geographical Locations	49:79	Human Breast Milk NMR Metabolomic Profile across Specific Geographical Locations and Its Association with the Milk Microbiota.
30248972	9	46	theme	metabolomic	1818:1828	arg1	profiles					1830:1837	specific milk metabolomic profiles	1804:1837	specific milk metabolomic profiles across geographical locations	1804:1867	Our results reveal specific milk metabolomic profiles across geographical locations and also highlight the potential interactions between human milk's metabolites and microbes.
30248972	6	47	theme	caesarean	1430:1438	arg1	section					1440:1446	caesarean section	1430:1446	caesarean section	1430:1446	Additionally, the effect of caesarean section on milk metabolome was dependent on the geographical region.
30248972	7	48	theme	milk	1547:1550	arg1	metabolites					1552:1562	human milk metabolites	1541:1562	human milk metabolites	1541:1562	Specific interrelations between human milk metabolites and microbiota were also identified.
30248972	4	49	theme	acid-associated	748:762	arg1	metabolites					764:774	fatty acid-associated metabolites	742:774	fatty acid-associated metabolites	742:774	Up to 68 metabolites, including amino acids, oligosaccharides, and fatty acid-associated metabolites, were identified in the milk NMR spectra.
30248972	2	50	theme	metabolic	414:422	arg1	profile					424:430	the nuclear magnetic resonance spectroscopy (NMR) metabolic profile	364:430	the nuclear magnetic resonance spectroscopy (NMR) metabolic profile of breast milk	364:445	This study aimed to investigate the impact of geographical location and mode of delivery on the nuclear magnetic resonance spectroscopy (NMR) metabolic profile of breast milk and its relationship with the milk microbiome.
30248972	5	51	theme	2-hydroxybutyrate	1114:1130	arg1	3-hydroxybutyrate					1133:1149	3-hydroxybutyrate	1133:1149	3-hydroxybutyrate	1133:1149	The metabolite profiles showed significant differences between geographical locations, with significant differences (p < 0.05) in the levels of galactose, lacto-N-fucopentaose III, lacto-N-fucopentaose I and 2-fucosyllactose, 3-fucosyllactose, lacto-N-difucohexaose II, lacto-N-fucopentaose III, 2-hydroxybutyrate, 3-hydroxybutyrate, proline, N-acetyl lysine, methyl-histidine, dimethylamine, kynurenine, urea, creatine and creatine phosphate, formate, lactate, acetate, phosphocholine, acetylcholine, LDL, VLDL, ethanolamine, riboflavin, hippurate, spermidine, spermine and uridine.
30248972	5	52	theme	galactose	962:970	arg1	levels					952:957	the levels	948:957	the levels of galactose	948:970	The metabolite profiles showed significant differences between geographical locations, with significant differences (p < 0.05) in the levels of galactose, lacto-N-fucopentaose III, lacto-N-fucopentaose I and 2-fucosyllactose, 3-fucosyllactose, lacto-N-difucohexaose II, lacto-N-fucopentaose III, 2-hydroxybutyrate, 3-hydroxybutyrate, proline, N-acetyl lysine, methyl-histidine, dimethylamine, kynurenine, urea, creatine and creatine phosphate, formate, lactate, acetate, phosphocholine, acetylcholine, LDL, VLDL, ethanolamine, riboflavin, hippurate, spermidine, spermine and uridine.
30248972	4	53	theme	fatty	742:746	arg1	metabolites					764:774	fatty acid-associated metabolites	742:774	fatty acid-associated metabolites	742:774	Up to 68 metabolites, including amino acids, oligosaccharides, and fatty acid-associated metabolites, were identified in the milk NMR spectra.
30248972	5	54	from	3-fucosyllactose	1044:1059	arg1	levels					952:957	the levels	948:957	the levels of galactose	948:970	The metabolite profiles showed significant differences between geographical locations, with significant differences (p < 0.05) in the levels of galactose, lacto-N-fucopentaose III, lacto-N-fucopentaose I and 2-fucosyllactose, 3-fucosyllactose, lacto-N-difucohexaose II, lacto-N-fucopentaose III, 2-hydroxybutyrate, 3-hydroxybutyrate, proline, N-acetyl lysine, methyl-histidine, dimethylamine, kynurenine, urea, creatine and creatine phosphate, formate, lactate, acetate, phosphocholine, acetylcholine, LDL, VLDL, ethanolamine, riboflavin, hippurate, spermidine, spermine and uridine.
30248972	4	55	dep	68	681:682	arg1	to					678:679	to	678:679	to	678:679	Up to 68 metabolites, including amino acids, oligosaccharides, and fatty acid-associated metabolites, were identified in the milk NMR spectra.
30248972	5	56	theme	p	935:935	arg1	differences					922:932	significant differences	910:932	significant differences (p < 0.05) in the levels of galactose	910:970	The metabolite profiles showed significant differences between geographical locations, with significant differences (p < 0.05) in the levels of galactose, lacto-N-fucopentaose III, lacto-N-fucopentaose I and 2-fucosyllactose, 3-fucosyllactose, lacto-N-difucohexaose II, lacto-N-fucopentaose III, 2-hydroxybutyrate, 3-hydroxybutyrate, proline, N-acetyl lysine, methyl-histidine, dimethylamine, kynurenine, urea, creatine and creatine phosphate, formate, lactate, acetate, phosphocholine, acetylcholine, LDL, VLDL, ethanolamine, riboflavin, hippurate, spermidine, spermine and uridine.
30248972	5	56	theme	p	935:935	arg1	<					937:937	p < 0.05	935:942	p < 0.05	935:942	The metabolite profiles showed significant differences between geographical locations, with significant differences (p < 0.05) in the levels of galactose, lacto-N-fucopentaose III, lacto-N-fucopentaose I and 2-fucosyllactose, 3-fucosyllactose, lacto-N-difucohexaose II, lacto-N-fucopentaose III, 2-hydroxybutyrate, 3-hydroxybutyrate, proline, N-acetyl lysine, methyl-histidine, dimethylamine, kynurenine, urea, creatine and creatine phosphate, formate, lactate, acetate, phosphocholine, acetylcholine, LDL, VLDL, ethanolamine, riboflavin, hippurate, spermidine, spermine and uridine.
30248972	3	57	theme	gene	578:581	arg1	sequencing					583:592	16S rRNA gene sequencing	569:592	16S rRNA gene sequencing	569:592	Human milk metabolic and microbiota profiles were determined using NMR and 16S rRNA gene sequencing, respectively, in 79 healthy women from Finland, Spain, South Africa, and China.
30248972	3	58	from	Spain	643:647	arg1	women					623:627	79 healthy women	612:627	79 healthy women from Finland, Spain, South Africa, and China	612:672	Human milk metabolic and microbiota profiles were determined using NMR and 16S rRNA gene sequencing, respectively, in 79 healthy women from Finland, Spain, South Africa, and China.
30248972	5	59	theme	creatine	1242:1249	arg1	phosphate					1251:1259	creatine phosphate	1242:1259	creatine phosphate	1242:1259	The metabolite profiles showed significant differences between geographical locations, with significant differences (p < 0.05) in the levels of galactose, lacto-N-fucopentaose III, lacto-N-fucopentaose I and 2-fucosyllactose, 3-fucosyllactose, lacto-N-difucohexaose II, lacto-N-fucopentaose III, 2-hydroxybutyrate, 3-hydroxybutyrate, proline, N-acetyl lysine, methyl-histidine, dimethylamine, kynurenine, urea, creatine and creatine phosphate, formate, lactate, acetate, phosphocholine, acetylcholine, LDL, VLDL, ethanolamine, riboflavin, hippurate, spermidine, spermine and uridine.
30248972	7	60	theme	human	1541:1545	arg1	metabolites					1552:1562	human milk metabolites	1541:1562	human milk metabolites	1541:1562	Specific interrelations between human milk metabolites and microbiota were also identified.
30248972	4	61	theme	amino	707:711	arg1	acids					713:717	amino acids	707:717	amino acids	707:717	Up to 68 metabolites, including amino acids, oligosaccharides, and fatty acid-associated metabolites, were identified in the milk NMR spectra.
30248972	2	62	with	profile	424:430	arg1	microbiome					482:491	the milk microbiome	473:491	the milk microbiome	473:491	This study aimed to investigate the impact of geographical location and mode of delivery on the nuclear magnetic resonance spectroscopy (NMR) metabolic profile of breast milk and its relationship with the milk microbiome.
30248972	1	63	theme	human	146:150	arg1	milk					159:162	human breast milk	146:162	human breast milk	146:162	The composition of human breast milk is highly variable, and it can be influenced by genetics, diet, lifestyle, and other environmental factors.
30248972	5	64	theme	significant	849:859	arg1	differences					861:871	significant differences	849:871	significant differences between geographical locations	849:902	The metabolite profiles showed significant differences between geographical locations, with significant differences (p < 0.05) in the levels of galactose, lacto-N-fucopentaose III, lacto-N-fucopentaose I and 2-fucosyllactose, 3-fucosyllactose, lacto-N-difucohexaose II, lacto-N-fucopentaose III, 2-hydroxybutyrate, 3-hydroxybutyrate, proline, N-acetyl lysine, methyl-histidine, dimethylamine, kynurenine, urea, creatine and creatine phosphate, formate, lactate, acetate, phosphocholine, acetylcholine, LDL, VLDL, ethanolamine, riboflavin, hippurate, spermidine, spermine and uridine.
30248972	5	65	from	lacto-N-fucopentaose	973:992	arg1	levels					952:957	the levels	948:957	the levels of galactose	948:970	The metabolite profiles showed significant differences between geographical locations, with significant differences (p < 0.05) in the levels of galactose, lacto-N-fucopentaose III, lacto-N-fucopentaose I and 2-fucosyllactose, 3-fucosyllactose, lacto-N-difucohexaose II, lacto-N-fucopentaose III, 2-hydroxybutyrate, 3-hydroxybutyrate, proline, N-acetyl lysine, methyl-histidine, dimethylamine, kynurenine, urea, creatine and creatine phosphate, formate, lactate, acetate, phosphocholine, acetylcholine, LDL, VLDL, ethanolamine, riboflavin, hippurate, spermidine, spermine and uridine.
30248972	3	66	theme	healthy	615:621	arg1	women					623:627	79 healthy women	612:627	79 healthy women from Finland, Spain, South Africa, and China	612:672	Human milk metabolic and microbiota profiles were determined using NMR and 16S rRNA gene sequencing, respectively, in 79 healthy women from Finland, Spain, South Africa, and China.
30248972	3	67	theme	16S	569:571	arg1	sequencing					583:592	16S rRNA gene sequencing	569:592	16S rRNA gene sequencing	569:592	Human milk metabolic and microbiota profiles were determined using NMR and 16S rRNA gene sequencing, respectively, in 79 healthy women from Finland, Spain, South Africa, and China.
30248972	9	68	theme	geographical	1846:1857	arg1	locations					1859:1867	geographical locations	1846:1867	geographical locations	1846:1867	Our results reveal specific milk metabolomic profiles across geographical locations and also highlight the potential interactions between human milk's metabolites and microbes.
30248972	0	69	theme	Geographical	58:69	arg1	Locations					71:79	Specific Geographical Locations	49:79	Specific Geographical Locations	49:79	Human Breast Milk NMR Metabolomic Profile across Specific Geographical Locations and Its Association with the Milk Microbiota.
30248972	5	70	theme	significant	910:920	arg1	differences					922:932	significant differences	910:932	significant differences (p < 0.05) in the levels of galactose	910:970	The metabolite profiles showed significant differences between geographical locations, with significant differences (p < 0.05) in the levels of galactose, lacto-N-fucopentaose III, lacto-N-fucopentaose I and 2-fucosyllactose, 3-fucosyllactose, lacto-N-difucohexaose II, lacto-N-fucopentaose III, 2-hydroxybutyrate, 3-hydroxybutyrate, proline, N-acetyl lysine, methyl-histidine, dimethylamine, kynurenine, urea, creatine and creatine phosphate, formate, lactate, acetate, phosphocholine, acetylcholine, LDL, VLDL, ethanolamine, riboflavin, hippurate, spermidine, spermine and uridine.
30248972	5	70	theme	significant	910:920	arg1	<					937:937	p < 0.05	935:942	p < 0.05	935:942	The metabolite profiles showed significant differences between geographical locations, with significant differences (p < 0.05) in the levels of galactose, lacto-N-fucopentaose III, lacto-N-fucopentaose I and 2-fucosyllactose, 3-fucosyllactose, lacto-N-difucohexaose II, lacto-N-fucopentaose III, 2-hydroxybutyrate, 3-hydroxybutyrate, proline, N-acetyl lysine, methyl-histidine, dimethylamine, kynurenine, urea, creatine and creatine phosphate, formate, lactate, acetate, phosphocholine, acetylcholine, LDL, VLDL, ethanolamine, riboflavin, hippurate, spermidine, spermine and uridine.
30248972	3	71	theme	rRNA	573:576	arg1	sequencing					583:592	16S rRNA gene sequencing	569:592	16S rRNA gene sequencing	569:592	Human milk metabolic and microbiota profiles were determined using NMR and 16S rRNA gene sequencing, respectively, in 79 healthy women from Finland, Spain, South Africa, and China.
30248972	2	72	theme	location	331:338	arg1	impact					308:313	the impact	304:313	the impact of geographical location and mode of delivery on the nuclear magnetic resonance spectroscopy (NMR) metabolic profile of breast milk and its relationship with the milk microbiome	304:491	This study aimed to investigate the impact of geographical location and mode of delivery on the nuclear magnetic resonance spectroscopy (NMR) metabolic profile of breast milk and its relationship with the milk microbiome.
30248972	5	73	from	differences	922:932	arg1	levels					952:957	the levels	948:957	the levels of galactose	948:970	The metabolite profiles showed significant differences between geographical locations, with significant differences (p < 0.05) in the levels of galactose, lacto-N-fucopentaose III, lacto-N-fucopentaose I and 2-fucosyllactose, 3-fucosyllactose, lacto-N-difucohexaose II, lacto-N-fucopentaose III, 2-hydroxybutyrate, 3-hydroxybutyrate, proline, N-acetyl lysine, methyl-histidine, dimethylamine, kynurenine, urea, creatine and creatine phosphate, formate, lactate, acetate, phosphocholine, acetylcholine, LDL, VLDL, ethanolamine, riboflavin, hippurate, spermidine, spermine and uridine.
30761104	11	0	from	microbiota	1557:1566	arg1	pigs					1579:1582	high FE pigs	1571:1582	high FE pigs	1571:1582	The fecal microbiota in high FE pigs have greater functional capacity to degrade dietary cellulose, polysaccharides, and protein and may have a greater abundance of microbes that can promote intestinal health.
30761104	1	1	theme	dietary	276:282	arg1	polysaccharides					284:298	dietary polysaccharides	276:298	dietary polysaccharides	276:298	Gut microbiota has indispensable roles in nutrient digestion and energy harvesting, especially in processing the indigestible components of dietary polysaccharides.
30761104	3	2	theme	shotgun	712:718	arg1	sequencing					732:741	shotgun metagenomic sequencing	712:741	shotgun metagenomic sequencing	712:741	In the current study, we performed a comparative analysis of the fecal microbiota in 50 commercial Duroc × (Landrace × Yorkshire) (DLY) pigs with polarizing FE using 16S rRNA gene sequencing and shotgun metagenomic sequencing.
30761104	3	3	with	pigs	653:656	arg1	FE					674:675	polarizing FE	663:675	polarizing FE	663:675	In the current study, we performed a comparative analysis of the fecal microbiota in 50 commercial Duroc × (Landrace × Yorkshire) (DLY) pigs with polarizing FE using 16S rRNA gene sequencing and shotgun metagenomic sequencing.
30761104	3	4	theme	comparative	554:564	arg1	analysis					566:573	a comparative analysis	552:573	a comparative analysis of the fecal microbiota in 50 commercial Duroc × (Landrace × Yorkshire) (DLY) pigs with polarizing FE	552:675	In the current study, we performed a comparative analysis of the fecal microbiota in 50 commercial Duroc × (Landrace × Yorkshire) (DLY) pigs with polarizing FE using 16S rRNA gene sequencing and shotgun metagenomic sequencing.
30761104	7	5	contain	had	1139:1141	arg1	pigs					1134:1137	The high-FE pigs	1122:1137	The high-FE pigs	1122:1137	The high-FE pigs had a greater abundance of OTUs that were from the Lachnospiraceae and Prevotellaceae families and the Escherichia-Shigella and Streptococcus genera than low-FE pigs.
30761104	7	5	contain	had	1139:1141	arg2	abundance					1153:1161	a greater abundance	1143:1161	a greater abundance of OTUs that were from the Lachnospiraceae and Prevotellaceae families and the Escherichia-Shigella and Streptococcus genera than low-FE pigs	1143:1303	The high-FE pigs had a greater abundance of OTUs that were from the Lachnospiraceae and Prevotellaceae families and the Escherichia-Shigella and Streptococcus genera than low-FE pigs.
30761104	2	6	theme	functional	338:347	arg1	capacity					349:356	functional capacity	338:356	functional capacity of the gut microbiome associated with feed efficiency (FE)	338:415	Searching for the microbial taxa and functional capacity of the gut microbiome associated with feed efficiency (FE) can provide important knowledge to increase profitability and sustainability of the swine industry.
30761104	4	7	theme	different	756:764	arg1	structure					786:794	a different microbial community structure	754:794	a different microbial community structure	754:794	There was a different microbial community structure in the fecal microbiota of pigs with different FE.
30761104	1	8	theme	energy	201:206	arg1	harvesting					208:217	energy harvesting	201:217	energy harvesting	201:217	Gut microbiota has indispensable roles in nutrient digestion and energy harvesting, especially in processing the indigestible components of dietary polysaccharides.
30761104	6	9	theme	Multiple	964:971	arg1	analysis					984:991	Multiple comparison analysis	964:991	Multiple comparison analysis	964:991	Multiple comparison analysis detected 8 OTUs with a significant difference or tendency toward a difference between high- and low-FE pigs (P < 0.01, q < 0.1).
30761104	3	10	theme	fecal	582:586	arg1	microbiota					588:597	the fecal microbiota	578:597	the fecal microbiota in 50 commercial Duroc × (Landrace × Yorkshire) (DLY) pigs with polarizing FE	578:675	In the current study, we performed a comparative analysis of the fecal microbiota in 50 commercial Duroc × (Landrace × Yorkshire) (DLY) pigs with polarizing FE using 16S rRNA gene sequencing and shotgun metagenomic sequencing.
30761104	3	11	theme	×	622:622	arg1	pigs					653:656	50 commercial Duroc × (Landrace × Yorkshire) (DLY) pigs	602:656	50 commercial Duroc × (Landrace × Yorkshire) (DLY) pigs with polarizing FE	602:675	In the current study, we performed a comparative analysis of the fecal microbiota in 50 commercial Duroc × (Landrace × Yorkshire) (DLY) pigs with polarizing FE using 16S rRNA gene sequencing and shotgun metagenomic sequencing.
30761104	5	12	theme	Random	847:852	arg1	analysis					861:868	Random forest analysis	847:868	Random forest analysis	847:868	Random forest analysis identified 24 operational taxonomic units (OTUs) as potential biomarkers to improve swine FE.
30761104	11	13	theme	dietary	1628:1634	arg1	cellulose					1636:1644	dietary cellulose	1628:1644	dietary cellulose	1628:1644	The fecal microbiota in high FE pigs have greater functional capacity to degrade dietary cellulose, polysaccharides, and protein and may have a greater abundance of microbes that can promote intestinal health.
30761104	3	14	theme	commercial	605:614	arg1	DLY					648:650	DLY	648:650	DLY	648:650	In the current study, we performed a comparative analysis of the fecal microbiota in 50 commercial Duroc × (Landrace × Yorkshire) (DLY) pigs with polarizing FE using 16S rRNA gene sequencing and shotgun metagenomic sequencing.
30761104	3	14	theme	commercial	605:614	arg1	×					622:622	50 commercial Duroc ×	602:622	50 commercial Duroc × (Landrace × Yorkshire) (DLY) pigs with polarizing FE	602:675	In the current study, we performed a comparative analysis of the fecal microbiota in 50 commercial Duroc × (Landrace × Yorkshire) (DLY) pigs with polarizing FE using 16S rRNA gene sequencing and shotgun metagenomic sequencing.
30761104	3	14	theme	commercial	605:614	arg1	Yorkshire					636:644	Landrace × Yorkshire	625:644	Landrace × Yorkshire	625:644	In the current study, we performed a comparative analysis of the fecal microbiota in 50 commercial Duroc × (Landrace × Yorkshire) (DLY) pigs with polarizing FE using 16S rRNA gene sequencing and shotgun metagenomic sequencing.
30761104	5	15	theme	potential	922:930	arg1	units					906:910	24 operational taxonomic units	881:910	24 operational taxonomic units (OTUs)	881:917	Random forest analysis identified 24 operational taxonomic units (OTUs) as potential biomarkers to improve swine FE.
30761104	5	15	theme	potential	922:930	arg1	biomarkers					932:941	potential biomarkers	922:941	potential biomarkers	922:941	Random forest analysis identified 24 operational taxonomic units (OTUs) as potential biomarkers to improve swine FE.
30761104	6	16	theme	significant	1016:1026	arg1	difference					1028:1037	a significant difference	1014:1037	a significant difference	1014:1037	Multiple comparison analysis detected 8 OTUs with a significant difference or tendency toward a difference between high- and low-FE pigs (P < 0.01, q < 0.1).
30761104	10	17	dep	high-	1524:1528	arg1	pigs					1541:1544	pigs	1541:1544	pigs	1541:1544	The functional capacity analysis found 18 KEGG pathways and CAZy EC activities that were different between high- and low-FE pigs.
30761104	0	18	from	Efficiency	101:110	arg1	Pigs					130:133	Commercial DLY Pigs	115:133	Commercial DLY Pigs	115:133	Exploring the Fecal Microbial Composition and Metagenomic Functional Capacities Associated With Feed Efficiency in Commercial DLY Pigs.
30761104	1	19	theme	indispensable	155:167	arg1	roles					169:173	indispensable roles	155:173	indispensable roles	155:173	Gut microbiota has indispensable roles in nutrient digestion and energy harvesting, especially in processing the indigestible components of dietary polysaccharides.
30761104	0	20	theme	Fecal	14:18	arg1	Composition					30:40	Fecal Microbial Composition	14:40	Fecal Microbial Composition	14:40	Exploring the Fecal Microbial Composition and Metagenomic Functional Capacities Associated With Feed Efficiency in Commercial DLY Pigs.
30761104	5	21	theme	taxonomic	896:904	arg1	units					906:910	24 operational taxonomic units	881:910	24 operational taxonomic units (OTUs)	881:917	Random forest analysis identified 24 operational taxonomic units (OTUs) as potential biomarkers to improve swine FE.
30761104	5	21	theme	taxonomic	896:904	arg1	biomarkers					932:941	potential biomarkers	922:941	potential biomarkers	922:941	Random forest analysis identified 24 operational taxonomic units (OTUs) as potential biomarkers to improve swine FE.
30761104	5	21	theme	taxonomic	896:904	arg1	OTUs					913:916	OTUs	913:916	OTUs	913:916	Random forest analysis identified 24 operational taxonomic units (OTUs) as potential biomarkers to improve swine FE.
30761104	10	22	theme	capacity	1432:1439	arg1	analysis					1441:1448	The functional capacity analysis	1417:1448	The functional capacity analysis	1417:1448	The functional capacity analysis found 18 KEGG pathways and CAZy EC activities that were different between high- and low-FE pigs.
30761104	7	23	theme	OTUs	1166:1169	arg1	abundance					1153:1161	a greater abundance	1143:1161	a greater abundance of OTUs that were from the Lachnospiraceae and Prevotellaceae families and the Escherichia-Shigella and Streptococcus genera than low-FE pigs	1143:1303	The high-FE pigs had a greater abundance of OTUs that were from the Lachnospiraceae and Prevotellaceae families and the Escherichia-Shigella and Streptococcus genera than low-FE pigs.
30761104	7	24	theme	Lachnospiraceae	1190:1204	arg1	families					1225:1232	the Lachnospiraceae and Prevotellaceae families	1186:1232	the Lachnospiraceae and Prevotellaceae families	1186:1232	The high-FE pigs had a greater abundance of OTUs that were from the Lachnospiraceae and Prevotellaceae families and the Escherichia-Shigella and Streptococcus genera than low-FE pigs.
30761104	4	25	theme	community	776:784	arg1	structure					786:794	a different microbial community structure	754:794	a different microbial community structure	754:794	There was a different microbial community structure in the fecal microbiota of pigs with different FE.
30761104	7	26	theme	greater	1145:1151	arg1	abundance					1153:1161	a greater abundance	1143:1161	a greater abundance of OTUs that were from the Lachnospiraceae and Prevotellaceae families and the Escherichia-Shigella and Streptococcus genera than low-FE pigs	1143:1303	The high-FE pigs had a greater abundance of OTUs that were from the Lachnospiraceae and Prevotellaceae families and the Escherichia-Shigella and Streptococcus genera than low-FE pigs.
30761104	11	27	theme	intestinal	1738:1747	arg1	health					1749:1754	intestinal health	1738:1754	intestinal health	1738:1754	The fecal microbiota in high FE pigs have greater functional capacity to degrade dietary cellulose, polysaccharides, and protein and may have a greater abundance of microbes that can promote intestinal health.
30761104	6	28	theme	high-	1079:1083	arg1	<					1114:1114	P < 0.01, q < 0.1	1102:1118	<	1114:1114	Multiple comparison analysis detected 8 OTUs with a significant difference or tendency toward a difference between high- and low-FE pigs (P < 0.01, q < 0.1).
30761104	6	28	theme	high-	1079:1083	arg1	pigs					1096:1099	high- and low-FE pigs	1079:1099	high- and low-FE pigs (P < 0.01, q < 0.1)	1079:1119	Multiple comparison analysis detected 8 OTUs with a significant difference or tendency toward a difference between high- and low-FE pigs (P < 0.01, q < 0.1).
30761104	10	29	theme	KEGG	1459:1462	arg1	pathways					1464:1471	18 KEGG pathways	1456:1471	18 KEGG pathways	1456:1471	The functional capacity analysis found 18 KEGG pathways and CAZy EC activities that were different between high- and low-FE pigs.
30761104	7	30	theme	high-FE	1126:1132	arg1	pigs					1134:1137	The high-FE pigs	1122:1137	The high-FE pigs	1122:1137	The high-FE pigs had a greater abundance of OTUs that were from the Lachnospiraceae and Prevotellaceae families and the Escherichia-Shigella and Streptococcus genera than low-FE pigs.
30761104	5	31	theme	swine	954:958	arg1	FE					960:961	swine FE	954:961	swine FE	954:961	Random forest analysis identified 24 operational taxonomic units (OTUs) as potential biomarkers to improve swine FE.
30761104	2	32	theme	industry	507:514	arg1	sustainability					479:492	sustainability	479:492	sustainability	479:492	Searching for the microbial taxa and functional capacity of the gut microbiome associated with feed efficiency (FE) can provide important knowledge to increase profitability and sustainability of the swine industry.
30761104	2	32	theme	industry	507:514	arg1	profitability					461:473	profitability	461:473	profitability	461:473	Searching for the microbial taxa and functional capacity of the gut microbiome associated with feed efficiency (FE) can provide important knowledge to increase profitability and sustainability of the swine industry.
30761104	10	33	theme	EC	1482:1483	arg1	activities					1485:1494	CAZy EC activities	1477:1494	CAZy EC activities	1477:1494	The functional capacity analysis found 18 KEGG pathways and CAZy EC activities that were different between high- and low-FE pigs.
30761104	2	34	theme	important	429:437	arg1	knowledge					439:447	important knowledge	429:447	important knowledge	429:447	Searching for the microbial taxa and functional capacity of the gut microbiome associated with feed efficiency (FE) can provide important knowledge to increase profitability and sustainability of the swine industry.
30761104	1	35	theme	nutrient	178:185	arg1	digestion					187:195	nutrient digestion	178:195	nutrient digestion	178:195	Gut microbiota has indispensable roles in nutrient digestion and energy harvesting, especially in processing the indigestible components of dietary polysaccharides.
30761104	7	36	theme	Streptococcus	1267:1279	arg1	genera					1281:1286	the Escherichia-Shigella and Streptococcus genera	1238:1286	the Escherichia-Shigella and Streptococcus genera	1238:1286	The high-FE pigs had a greater abundance of OTUs that were from the Lachnospiraceae and Prevotellaceae families and the Escherichia-Shigella and Streptococcus genera than low-FE pigs.
30761104	2	37	theme	microbiome	369:378	arg1	capacity					349:356	functional capacity	338:356	functional capacity of the gut microbiome associated with feed efficiency (FE)	338:415	Searching for the microbial taxa and functional capacity of the gut microbiome associated with feed efficiency (FE) can provide important knowledge to increase profitability and sustainability of the swine industry.
30761104	0	38	theme	Metagenomic	46:56	arg1	Capacities					69:78	Metagenomic Functional Capacities	46:78	Metagenomic Functional Capacities	46:78	Exploring the Fecal Microbial Composition and Metagenomic Functional Capacities Associated With Feed Efficiency in Commercial DLY Pigs.
30761104	0	39	dep	Composition	30:40	arg1	the					10:12	the	10:12	the	10:12	Exploring the Fecal Microbial Composition and Metagenomic Functional Capacities Associated With Feed Efficiency in Commercial DLY Pigs.
30761104	7	40	theme	Escherichia-Shigella	1242:1261	arg1	genera					1281:1286	the Escherichia-Shigella and Streptococcus genera	1238:1286	the Escherichia-Shigella and Streptococcus genera	1238:1286	The high-FE pigs had a greater abundance of OTUs that were from the Lachnospiraceae and Prevotellaceae families and the Escherichia-Shigella and Streptococcus genera than low-FE pigs.
30761104	3	41	theme	Landrace	625:632	arg1	×					622:622	50 commercial Duroc ×	602:622	50 commercial Duroc × (Landrace × Yorkshire) (DLY) pigs with polarizing FE	602:675	In the current study, we performed a comparative analysis of the fecal microbiota in 50 commercial Duroc × (Landrace × Yorkshire) (DLY) pigs with polarizing FE using 16S rRNA gene sequencing and shotgun metagenomic sequencing.
30761104	3	41	theme	Landrace	625:632	arg1	Yorkshire					636:644	Landrace × Yorkshire	625:644	Landrace × Yorkshire	625:644	In the current study, we performed a comparative analysis of the fecal microbiota in 50 commercial Duroc × (Landrace × Yorkshire) (DLY) pigs with polarizing FE using 16S rRNA gene sequencing and shotgun metagenomic sequencing.
30761104	11	42	theme	microbes	1712:1719	arg1	abundance					1699:1707	a greater abundance	1689:1707	a greater abundance of microbes that can promote intestinal health	1689:1754	The fecal microbiota in high FE pigs have greater functional capacity to degrade dietary cellulose, polysaccharides, and protein and may have a greater abundance of microbes that can promote intestinal health.
30761104	11	43	theme	FE	1576:1577	arg1	pigs					1579:1582	high FE pigs	1571:1582	high FE pigs	1571:1582	The fecal microbiota in high FE pigs have greater functional capacity to degrade dietary cellulose, polysaccharides, and protein and may have a greater abundance of microbes that can promote intestinal health.
30761104	6	44	theme	<	1104:1104	arg1	<					1114:1114	P < 0.01, q < 0.1	1102:1118	<	1114:1114	Multiple comparison analysis detected 8 OTUs with a significant difference or tendency toward a difference between high- and low-FE pigs (P < 0.01, q < 0.1).
30761104	6	44	theme	<	1104:1104	arg1	pigs					1096:1099	high- and low-FE pigs	1079:1099	high- and low-FE pigs (P < 0.01, q < 0.1)	1079:1119	Multiple comparison analysis detected 8 OTUs with a significant difference or tendency toward a difference between high- and low-FE pigs (P < 0.01, q < 0.1).
30761104	3	45	from	pigs	653:656	arg1	analysis					566:573	a comparative analysis	552:573	a comparative analysis of the fecal microbiota in 50 commercial Duroc × (Landrace × Yorkshire) (DLY) pigs with polarizing FE	552:675	In the current study, we performed a comparative analysis of the fecal microbiota in 50 commercial Duroc × (Landrace × Yorkshire) (DLY) pigs with polarizing FE using 16S rRNA gene sequencing and shotgun metagenomic sequencing.
30761104	4	46	theme	fecal	803:807	arg1	microbiota					809:818	the fecal microbiota	799:818	the fecal microbiota of pigs with different FE	799:844	There was a different microbial community structure in the fecal microbiota of pigs with different FE.
30761104	1	47	theme	indigestible	249:260	arg1	components					262:271	the indigestible components	245:271	processing the indigestible components of dietary polysaccharides	234:298	Gut microbiota has indispensable roles in nutrient digestion and energy harvesting, especially in processing the indigestible components of dietary polysaccharides.
30761104	7	48	theme	Prevotellaceae	1210:1223	arg1	families					1225:1232	the Lachnospiraceae and Prevotellaceae families	1186:1232	the Lachnospiraceae and Prevotellaceae families	1186:1232	The high-FE pigs had a greater abundance of OTUs that were from the Lachnospiraceae and Prevotellaceae families and the Escherichia-Shigella and Streptococcus genera than low-FE pigs.
30761104	3	49	theme	gene	692:695	arg1	sequencing					697:706	16S rRNA gene sequencing	683:706	16S rRNA gene sequencing	683:706	In the current study, we performed a comparative analysis of the fecal microbiota in 50 commercial Duroc × (Landrace × Yorkshire) (DLY) pigs with polarizing FE using 16S rRNA gene sequencing and shotgun metagenomic sequencing.
30761104	11	50	theme	functional	1597:1606	arg1	capacity					1608:1615	greater functional capacity	1589:1615	greater functional capacity to degrade dietary cellulose, polysaccharides, and protein	1589:1674	The fecal microbiota in high FE pigs have greater functional capacity to degrade dietary cellulose, polysaccharides, and protein and may have a greater abundance of microbes that can promote intestinal health.
30761104	6	51	theme	low-FE	1089:1094	arg1	<					1114:1114	P < 0.01, q < 0.1	1102:1118	<	1114:1114	Multiple comparison analysis detected 8 OTUs with a significant difference or tendency toward a difference between high- and low-FE pigs (P < 0.01, q < 0.1).
30761104	6	51	theme	low-FE	1089:1094	arg1	pigs					1096:1099	high- and low-FE pigs	1079:1099	high- and low-FE pigs (P < 0.01, q < 0.1)	1079:1119	Multiple comparison analysis detected 8 OTUs with a significant difference or tendency toward a difference between high- and low-FE pigs (P < 0.01, q < 0.1).
30761104	3	52	theme	16S	683:685	arg1	sequencing					697:706	16S rRNA gene sequencing	683:706	16S rRNA gene sequencing	683:706	In the current study, we performed a comparative analysis of the fecal microbiota in 50 commercial Duroc × (Landrace × Yorkshire) (DLY) pigs with polarizing FE using 16S rRNA gene sequencing and shotgun metagenomic sequencing.
30761104	2	53	theme	microbial	319:327	arg1	taxa					329:332	the microbial taxa	315:332	the microbial taxa	315:332	Searching for the microbial taxa and functional capacity of the gut microbiome associated with feed efficiency (FE) can provide important knowledge to increase profitability and sustainability of the swine industry.
30761104	1	54	theme	polysaccharides	284:298	arg1	components					262:271	the indigestible components	245:271	processing the indigestible components of dietary polysaccharides	234:298	Gut microbiota has indispensable roles in nutrient digestion and energy harvesting, especially in processing the indigestible components of dietary polysaccharides.
30761104	3	55	theme	metagenomic	720:730	arg1	sequencing					732:741	shotgun metagenomic sequencing	712:741	shotgun metagenomic sequencing	712:741	In the current study, we performed a comparative analysis of the fecal microbiota in 50 commercial Duroc × (Landrace × Yorkshire) (DLY) pigs with polarizing FE using 16S rRNA gene sequencing and shotgun metagenomic sequencing.
30761104	5	56	theme	forest	854:859	arg1	analysis					861:868	Random forest analysis	847:868	Random forest analysis	847:868	Random forest analysis identified 24 operational taxonomic units (OTUs) as potential biomarkers to improve swine FE.
30761104	4	57	theme	different	833:841	arg1	FE					843:844	different FE	833:844	different FE	833:844	There was a different microbial community structure in the fecal microbiota of pigs with different FE.
30761104	1	58	contain	has	151:153	arg2	roles					169:173	indispensable roles	155:173	indispensable roles	155:173	Gut microbiota has indispensable roles in nutrient digestion and energy harvesting, especially in processing the indigestible components of dietary polysaccharides.
30761104	1	58	contain	has	151:153	arg1	microbiota					140:149	Gut microbiota	136:149	Gut microbiota	136:149	Gut microbiota has indispensable roles in nutrient digestion and energy harvesting, especially in processing the indigestible components of dietary polysaccharides.
30761104	3	59	theme	microbiota	588:597	arg1	analysis					566:573	a comparative analysis	552:573	a comparative analysis of the fecal microbiota in 50 commercial Duroc × (Landrace × Yorkshire) (DLY) pigs with polarizing FE	552:675	In the current study, we performed a comparative analysis of the fecal microbiota in 50 commercial Duroc × (Landrace × Yorkshire) (DLY) pigs with polarizing FE using 16S rRNA gene sequencing and shotgun metagenomic sequencing.
30761104	6	60	theme	comparison	973:982	arg1	analysis					984:991	Multiple comparison analysis	964:991	Multiple comparison analysis	964:991	Multiple comparison analysis detected 8 OTUs with a significant difference or tendency toward a difference between high- and low-FE pigs (P < 0.01, q < 0.1).
30761104	11	61	theme	fecal	1551:1555	arg1	microbiota					1557:1566	The fecal microbiota	1547:1566	The fecal microbiota in high FE pigs	1547:1582	The fecal microbiota in high FE pigs have greater functional capacity to degrade dietary cellulose, polysaccharides, and protein and may have a greater abundance of microbes that can promote intestinal health.
30761104	2	62	theme	gut	365:367	arg1	microbiome					369:378	the gut microbiome	361:378	the gut microbiome	361:378	Searching for the microbial taxa and functional capacity of the gut microbiome associated with feed efficiency (FE) can provide important knowledge to increase profitability and sustainability of the swine industry.
30761104	3	63	theme	Duroc	616:620	arg1	DLY					648:650	DLY	648:650	DLY	648:650	In the current study, we performed a comparative analysis of the fecal microbiota in 50 commercial Duroc × (Landrace × Yorkshire) (DLY) pigs with polarizing FE using 16S rRNA gene sequencing and shotgun metagenomic sequencing.
30761104	3	63	theme	Duroc	616:620	arg1	×					622:622	50 commercial Duroc ×	602:622	50 commercial Duroc × (Landrace × Yorkshire) (DLY) pigs with polarizing FE	602:675	In the current study, we performed a comparative analysis of the fecal microbiota in 50 commercial Duroc × (Landrace × Yorkshire) (DLY) pigs with polarizing FE using 16S rRNA gene sequencing and shotgun metagenomic sequencing.
30761104	3	63	theme	Duroc	616:620	arg1	Yorkshire					636:644	Landrace × Yorkshire	625:644	Landrace × Yorkshire	625:644	In the current study, we performed a comparative analysis of the fecal microbiota in 50 commercial Duroc × (Landrace × Yorkshire) (DLY) pigs with polarizing FE using 16S rRNA gene sequencing and shotgun metagenomic sequencing.
30761104	1	64	dep	processing	234:243	arg1	components					262:271	the indigestible components	245:271	processing the indigestible components of dietary polysaccharides	234:298	Gut microbiota has indispensable roles in nutrient digestion and energy harvesting, especially in processing the indigestible components of dietary polysaccharides.
30761104	11	65	contain	have	1584:1587	arg2	capacity					1608:1615	greater functional capacity	1589:1615	greater functional capacity to degrade dietary cellulose, polysaccharides, and protein	1589:1674	The fecal microbiota in high FE pigs have greater functional capacity to degrade dietary cellulose, polysaccharides, and protein and may have a greater abundance of microbes that can promote intestinal health.
30761104	11	65	contain	have	1584:1587	arg1	microbiota					1557:1566	The fecal microbiota	1547:1566	The fecal microbiota in high FE pigs	1547:1582	The fecal microbiota in high FE pigs have greater functional capacity to degrade dietary cellulose, polysaccharides, and protein and may have a greater abundance of microbes that can promote intestinal health.
30761104	3	66	theme	×	634:634	arg1	×					622:622	50 commercial Duroc ×	602:622	50 commercial Duroc × (Landrace × Yorkshire) (DLY) pigs with polarizing FE	602:675	In the current study, we performed a comparative analysis of the fecal microbiota in 50 commercial Duroc × (Landrace × Yorkshire) (DLY) pigs with polarizing FE using 16S rRNA gene sequencing and shotgun metagenomic sequencing.
30761104	3	66	theme	×	634:634	arg1	Yorkshire					636:644	Landrace × Yorkshire	625:644	Landrace × Yorkshire	625:644	In the current study, we performed a comparative analysis of the fecal microbiota in 50 commercial Duroc × (Landrace × Yorkshire) (DLY) pigs with polarizing FE using 16S rRNA gene sequencing and shotgun metagenomic sequencing.
30761104	1	67	theme	Gut	136:138	arg1	microbiota					140:149	Gut microbiota	136:149	Gut microbiota	136:149	Gut microbiota has indispensable roles in nutrient digestion and energy harvesting, especially in processing the indigestible components of dietary polysaccharides.
30761104	8	68	theme	Streptococcus	1320:1332	arg1	subsp					1347:1351	A sub-species Streptococcus gallolyticus subsp	1306:1351	A sub-species Streptococcus gallolyticus subsp	1306:1351	A sub-species Streptococcus gallolyticus subsp.
30761104	3	69	from	microbiota	588:597	arg1	pigs					653:656	50 commercial Duroc × (Landrace × Yorkshire) (DLY) pigs	602:656	50 commercial Duroc × (Landrace × Yorkshire) (DLY) pigs with polarizing FE	602:675	In the current study, we performed a comparative analysis of the fecal microbiota in 50 commercial Duroc × (Landrace × Yorkshire) (DLY) pigs with polarizing FE using 16S rRNA gene sequencing and shotgun metagenomic sequencing.
30761104	4	70	with	pigs	823:826	arg1	FE					843:844	different FE	833:844	different FE	833:844	There was a different microbial community structure in the fecal microbiota of pigs with different FE.
30761104	5	71	theme	operational	884:894	arg1	units					906:910	24 operational taxonomic units	881:910	24 operational taxonomic units (OTUs)	881:917	Random forest analysis identified 24 operational taxonomic units (OTUs) as potential biomarkers to improve swine FE.
30761104	5	71	theme	operational	884:894	arg1	biomarkers					932:941	potential biomarkers	922:941	potential biomarkers	922:941	Random forest analysis identified 24 operational taxonomic units (OTUs) as potential biomarkers to improve swine FE.
30761104	5	71	theme	operational	884:894	arg1	OTUs					913:916	OTUs	913:916	OTUs	913:916	Random forest analysis identified 24 operational taxonomic units (OTUs) as potential biomarkers to improve swine FE.
30761104	0	72	theme	Feed	96:99	arg1	Efficiency					101:110	Feed Efficiency	96:110	Feed Efficiency in Commercial DLY Pigs	96:133	Exploring the Fecal Microbial Composition and Metagenomic Functional Capacities Associated With Feed Efficiency in Commercial DLY Pigs.
30761104	3	73	from	analysis	566:573	arg1	pigs					653:656	50 commercial Duroc × (Landrace × Yorkshire) (DLY) pigs	602:656	50 commercial Duroc × (Landrace × Yorkshire) (DLY) pigs with polarizing FE	602:675	In the current study, we performed a comparative analysis of the fecal microbiota in 50 commercial Duroc × (Landrace × Yorkshire) (DLY) pigs with polarizing FE using 16S rRNA gene sequencing and shotgun metagenomic sequencing.
30761104	11	74	contain	have	1684:1687	arg2	abundance					1699:1707	a greater abundance	1689:1707	a greater abundance of microbes that can promote intestinal health	1689:1754	The fecal microbiota in high FE pigs have greater functional capacity to degrade dietary cellulose, polysaccharides, and protein and may have a greater abundance of microbes that can promote intestinal health.
30761104	11	74	contain	have	1684:1687	arg1	microbiota					1557:1566	The fecal microbiota	1547:1566	The fecal microbiota in high FE pigs	1547:1582	The fecal microbiota in high FE pigs have greater functional capacity to degrade dietary cellulose, polysaccharides, and protein and may have a greater abundance of microbes that can promote intestinal health.
30761104	10	75	theme	functional	1421:1430	arg1	capacity					1432:1439	The functional capacity	1417:1439	The functional capacity analysis	1417:1448	The functional capacity analysis found 18 KEGG pathways and CAZy EC activities that were different between high- and low-FE pigs.
30761104	8	76	theme	sub-species	1308:1318	arg1	subsp					1347:1351	A sub-species Streptococcus gallolyticus subsp	1306:1351	A sub-species Streptococcus gallolyticus subsp	1306:1351	A sub-species Streptococcus gallolyticus subsp.
30761104	0	77	theme	DLY	126:128	arg1	Pigs					130:133	Commercial DLY Pigs	115:133	Commercial DLY Pigs	115:133	Exploring the Fecal Microbial Composition and Metagenomic Functional Capacities Associated With Feed Efficiency in Commercial DLY Pigs.
30761104	8	78	theme	gallolyticus	1334:1345	arg1	subsp					1347:1351	A sub-species Streptococcus gallolyticus subsp	1306:1351	A sub-species Streptococcus gallolyticus subsp	1306:1351	A sub-species Streptococcus gallolyticus subsp.
30761104	7	79	theme	low-FE	1293:1298	arg1	pigs					1300:1303	low-FE pigs	1293:1303	low-FE pigs	1293:1303	The high-FE pigs had a greater abundance of OTUs that were from the Lachnospiraceae and Prevotellaceae families and the Escherichia-Shigella and Streptococcus genera than low-FE pigs.
30761104	0	80	theme	Microbial	20:28	arg1	Composition					30:40	Fecal Microbial Composition	14:40	Fecal Microbial Composition	14:40	Exploring the Fecal Microbial Composition and Metagenomic Functional Capacities Associated With Feed Efficiency in Commercial DLY Pigs.
30761104	2	81	theme	swine	501:505	arg1	industry					507:514	the swine industry	497:514	the swine industry	497:514	Searching for the microbial taxa and functional capacity of the gut microbiome associated with feed efficiency (FE) can provide important knowledge to increase profitability and sustainability of the swine industry.
30761104	10	82	theme	CAZy	1477:1480	arg1	activities					1485:1494	CAZy EC activities	1477:1494	CAZy EC activities	1477:1494	The functional capacity analysis found 18 KEGG pathways and CAZy EC activities that were different between high- and low-FE pigs.
30761104	0	83	theme	Commercial	115:124	arg1	Pigs					130:133	Commercial DLY Pigs	115:133	Commercial DLY Pigs	115:133	Exploring the Fecal Microbial Composition and Metagenomic Functional Capacities Associated With Feed Efficiency in Commercial DLY Pigs.
30761104	0	84	theme	Functional	58:67	arg1	Capacities					69:78	Metagenomic Functional Capacities	46:78	Metagenomic Functional Capacities	46:78	Exploring the Fecal Microbial Composition and Metagenomic Functional Capacities Associated With Feed Efficiency in Commercial DLY Pigs.
30761104	12	85	theme	gut	1837:1839	arg1	microbiome					1841:1850	the gut microbiome	1833:1850	the gut microbiome	1833:1850	These results provided insights for improving porcine FE through modulating the gut microbiome.
30761104	12	86	theme	porcine	1803:1809	arg1	FE					1811:1812	porcine FE	1803:1812	porcine FE	1803:1812	These results provided insights for improving porcine FE through modulating the gut microbiome.
30761104	3	87	theme	polarizing	663:672	arg1	FE					674:675	polarizing FE	663:675	polarizing FE	663:675	In the current study, we performed a comparative analysis of the fecal microbiota in 50 commercial Duroc × (Landrace × Yorkshire) (DLY) pigs with polarizing FE using 16S rRNA gene sequencing and shotgun metagenomic sequencing.
30761104	4	88	theme	pigs	823:826	arg1	microbiota					809:818	the fecal microbiota	799:818	the fecal microbiota of pigs with different FE	799:844	There was a different microbial community structure in the fecal microbiota of pigs with different FE.
30761104	11	89	theme	high	1571:1574	arg1	pigs					1579:1582	high FE pigs	1571:1582	high FE pigs	1571:1582	The fecal microbiota in high FE pigs have greater functional capacity to degrade dietary cellulose, polysaccharides, and protein and may have a greater abundance of microbes that can promote intestinal health.
30761104	3	90	theme	current	524:530	arg1	study					532:536	the current study	520:536	the current study	520:536	In the current study, we performed a comparative analysis of the fecal microbiota in 50 commercial Duroc × (Landrace × Yorkshire) (DLY) pigs with polarizing FE using 16S rRNA gene sequencing and shotgun metagenomic sequencing.
30761104	4	91	theme	microbial	766:774	arg1	structure					786:794	a different microbial community structure	754:794	a different microbial community structure	754:794	There was a different microbial community structure in the fecal microbiota of pigs with different FE.
30761104	11	92	theme	greater	1691:1697	arg1	abundance					1699:1707	a greater abundance	1689:1707	a greater abundance of microbes that can promote intestinal health	1689:1754	The fecal microbiota in high FE pigs have greater functional capacity to degrade dietary cellulose, polysaccharides, and protein and may have a greater abundance of microbes that can promote intestinal health.
30761104	9	93	theme	important	1379:1387	arg1	candidate					1389:1397	an important candidate	1376:1397	an important candidate for improving FE	1376:1414	gallolyticus could be an important candidate for improving FE.
30761104	9	93	theme	important	1379:1387	arg1	gallolyticus					1354:1365	gallolyticus	1354:1365	gallolyticus	1354:1365	gallolyticus could be an important candidate for improving FE.
30761104	6	94	theme	P	1102:1102	arg1	<					1114:1114	P < 0.01, q < 0.1	1102:1118	<	1114:1114	Multiple comparison analysis detected 8 OTUs with a significant difference or tendency toward a difference between high- and low-FE pigs (P < 0.01, q < 0.1).
30761104	6	94	theme	P	1102:1102	arg1	pigs					1096:1099	high- and low-FE pigs	1079:1099	high- and low-FE pigs (P < 0.01, q < 0.1)	1079:1119	Multiple comparison analysis detected 8 OTUs with a significant difference or tendency toward a difference between high- and low-FE pigs (P < 0.01, q < 0.1).
30761104	3	95	theme	rRNA	687:690	arg1	sequencing					697:706	16S rRNA gene sequencing	683:706	16S rRNA gene sequencing	683:706	In the current study, we performed a comparative analysis of the fecal microbiota in 50 commercial Duroc × (Landrace × Yorkshire) (DLY) pigs with polarizing FE using 16S rRNA gene sequencing and shotgun metagenomic sequencing.
30761104	11	96	theme	greater	1589:1595	arg1	capacity					1608:1615	greater functional capacity	1589:1615	greater functional capacity to degrade dietary cellulose, polysaccharides, and protein	1589:1674	The fecal microbiota in high FE pigs have greater functional capacity to degrade dietary cellulose, polysaccharides, and protein and may have a greater abundance of microbes that can promote intestinal health.
30761104	2	97	theme	feed	396:399	arg1	FE					413:414	FE	413:414	FE	413:414	Searching for the microbial taxa and functional capacity of the gut microbiome associated with feed efficiency (FE) can provide important knowledge to increase profitability and sustainability of the swine industry.
30761104	2	97	theme	feed	396:399	arg1	efficiency					401:410	feed efficiency	396:410	feed efficiency (FE)	396:415	Searching for the microbial taxa and functional capacity of the gut microbiome associated with feed efficiency (FE) can provide important knowledge to increase profitability and sustainability of the swine industry.
31361770	0	0	theme	internode	89:97	arg1	development					99:109	maize internode development	83:109	maize internode development	83:109	Changes in cell walls lignification, feruloylation and p-coumaroylation throughout maize internode development.
31361770	5	1	theme	walls	789:793	arg1	development					795:805	internode cell walls development	774:805	internode cell walls development in maize before flowering	774:831	We combined biochemical and histological approaches and revealed that internode cell walls development in maize before flowering is characterized by the rapid deposition of secondary cell walls components and robust lignification in both the pith and the rind.
31361770	0	2	theme	maize	83:87	arg1	development					99:109	maize internode development	83:109	maize internode development	83:109	Changes in cell walls lignification, feruloylation and p-coumaroylation throughout maize internode development.
31361770	3	3	theme	distinct	502:509	arg1	genotypes					517:525	three distinct maize genotypes	496:525	three distinct maize genotypes	496:525	Here, we studied the patterns of cell walls establishment in the internode supporting the ear in three distinct maize genotypes.
31361770	4	4	theme	cell	581:584	arg1	walls					586:590	the internode cell walls	567:590	the internode cell walls in terms of its composition	567:618	The developmental patterns observed in the internode cell walls in terms of its composition are reported with an emphasis on lignification, p-coumaroylation and feruloylation.
31361770	0	5	from	Changes	0:6	arg1	walls					16:20	cell walls	11:20	cell walls	11:20	Changes in cell walls lignification, feruloylation and p-coumaroylation throughout maize internode development.
31361770	4	6	located	observed	555:562	arg2	patterns					546:553	The developmental patterns	528:553	The developmental patterns observed in the internode cell walls in terms of its composition	528:618	The developmental patterns observed in the internode cell walls in terms of its composition are reported with an emphasis on lignification, p-coumaroylation and feruloylation.
31361770	4	6	located	observed	555:562	arg1	walls					586:590	the internode cell walls	567:590	the internode cell walls in terms of its composition	567:618	The developmental patterns observed in the internode cell walls in terms of its composition are reported with an emphasis on lignification, p-coumaroylation and feruloylation.
31361770	7	7	dep	Terashima	1263:1271	arg1	al					1276:1277	Terashima et al	1263:1277	Terashima et al. (1993) for cell walls development in grass	1263:1321	We conclude the paper by proposing a revised spatiotemporal model based on that proposed by Terashima et al. (1993) for cell walls development in grass.
31361770	7	8	theme	revised	1208:1214	arg1	model					1231:1235	a revised spatiotemporal model	1206:1235	a revised spatiotemporal model based on that proposed by Terashima et al. (1993) for cell walls development in grass	1206:1321	We conclude the paper by proposing a revised spatiotemporal model based on that proposed by Terashima et al. (1993) for cell walls development in grass.
31361770	2	9	from	walls	238:242	arg1	grass					247:251	grass	247:251	grass	247:251	In the cell walls in grass, the accessibility of structural polysaccharides is limited by the cell walls structure and composition mainly as a result of phenolic compounds.
31361770	5	10	theme	cell	887:890	arg1	walls					892:896	secondary cell walls	877:896	secondary cell walls components and robust lignification	877:932	We combined biochemical and histological approaches and revealed that internode cell walls development in maize before flowering is characterized by the rapid deposition of secondary cell walls components and robust lignification in both the pith and the rind.
31361770	4	11	from	walls	586:590	arg1	terms					595:599	terms	595:599	terms of its composition	595:618	The developmental patterns observed in the internode cell walls in terms of its composition are reported with an emphasis on lignification, p-coumaroylation and feruloylation.
31361770	5	12	dep	walls	892:896	arg1	components					898:907	components	898:907	components	898:907	We combined biochemical and histological approaches and revealed that internode cell walls development in maize before flowering is characterized by the rapid deposition of secondary cell walls components and robust lignification in both the pith and the rind.
31361770	5	12	dep	walls	892:896	arg1	lignification					920:932	robust lignification	913:932	robust lignification	913:932	We combined biochemical and histological approaches and revealed that internode cell walls development in maize before flowering is characterized by the rapid deposition of secondary cell walls components and robust lignification in both the pith and the rind.
31361770	7	13	theme	cell	1291:1294	arg1	development					1302:1312	cell walls development	1291:1312	cell walls development in grass	1291:1321	We conclude the paper by proposing a revised spatiotemporal model based on that proposed by Terashima et al. (1993) for cell walls development in grass.
31361770	2	14	theme	polysaccharides	286:300	arg1	result					369:374	a result	367:374	a result of phenolic compounds	367:396	In the cell walls in grass, the accessibility of structural polysaccharides is limited by the cell walls structure and composition mainly as a result of phenolic compounds.
31361770	2	14	theme	polysaccharides	286:300	arg1	accessibility					258:270	the accessibility	254:270	the accessibility of structural polysaccharides	254:300	In the cell walls in grass, the accessibility of structural polysaccharides is limited by the cell walls structure and composition mainly as a result of phenolic compounds.
31361770	7	15	theme	walls	1296:1300	arg1	development					1302:1312	cell walls development	1291:1312	cell walls development in grass	1291:1321	We conclude the paper by proposing a revised spatiotemporal model based on that proposed by Terashima et al. (1993) for cell walls development in grass.
31361770	1	16	theme	several	179:185	arg1	components					187:196	several components	179:196	several components	179:196	Plant cell walls development is an integrated process during which several components are deposited successively.
31361770	2	17	theme	structural	275:284	arg1	polysaccharides					286:300	structural polysaccharides	275:300	structural polysaccharides	275:300	In the cell walls in grass, the accessibility of structural polysaccharides is limited by the cell walls structure and composition mainly as a result of phenolic compounds.
31361770	6	18	theme	deposited	1097:1105	arg1	p-coumaroylated					1154:1168	p-coumaroylated	1154:1168	p-coumaroylated	1154:1168	After flowering and until silage maturity, the slow deposition of secondary walls components occurs in the cortical region, and the deposited lignins are rich in β-O-4 bonds and are highly p-coumaroylated.
31361770	6	18	theme	deposited	1097:1105	arg1	rich					1119:1122	rich	1119:1122	rich	1119:1122	After flowering and until silage maturity, the slow deposition of secondary walls components occurs in the cortical region, and the deposited lignins are rich in β-O-4 bonds and are highly p-coumaroylated.
31361770	6	18	theme	deposited	1097:1105	arg1	lignins					1107:1113	the deposited lignins	1093:1113	the deposited lignins	1093:1113	After flowering and until silage maturity, the slow deposition of secondary walls components occurs in the cortical region, and the deposited lignins are rich in β-O-4 bonds and are highly p-coumaroylated.
31361770	3	19	theme	walls	437:441	arg1	patterns					420:427	the patterns	416:427	the patterns of cell walls establishment in the internode supporting the ear in three distinct maize genotypes	416:525	Here, we studied the patterns of cell walls establishment in the internode supporting the ear in three distinct maize genotypes.
31361770	2	20	theme	compounds	388:396	arg1	accessibility					258:270	the accessibility	254:270	the accessibility of structural polysaccharides	254:300	In the cell walls in grass, the accessibility of structural polysaccharides is limited by the cell walls structure and composition mainly as a result of phenolic compounds.
31361770	2	20	theme	compounds	388:396	arg1	result					369:374	a result	367:374	a result of phenolic compounds	367:396	In the cell walls in grass, the accessibility of structural polysaccharides is limited by the cell walls structure and composition mainly as a result of phenolic compounds.
31361770	6	21	theme	walls	1041:1045	arg1	components					1047:1056	secondary walls components	1031:1056	secondary walls components	1031:1056	After flowering and until silage maturity, the slow deposition of secondary walls components occurs in the cortical region, and the deposited lignins are rich in β-O-4 bonds and are highly p-coumaroylated.
31361770	7	22	theme	spatiotemporal	1216:1229	arg1	model					1231:1235	a revised spatiotemporal model	1206:1235	a revised spatiotemporal model based on that proposed by Terashima et al. (1993) for cell walls development in grass	1206:1321	We conclude the paper by proposing a revised spatiotemporal model based on that proposed by Terashima et al. (1993) for cell walls development in grass.
31361770	3	23	from	ear	489:491	arg1	genotypes					517:525	three distinct maize genotypes	496:525	three distinct maize genotypes	496:525	Here, we studied the patterns of cell walls establishment in the internode supporting the ear in three distinct maize genotypes.
31361770	2	24	theme	cell	320:323	arg1	composition					345:355	composition	345:355	composition	345:355	In the cell walls in grass, the accessibility of structural polysaccharides is limited by the cell walls structure and composition mainly as a result of phenolic compounds.
31361770	2	24	theme	cell	320:323	arg1	structure					331:339	structure	331:339	structure	331:339	In the cell walls in grass, the accessibility of structural polysaccharides is limited by the cell walls structure and composition mainly as a result of phenolic compounds.
31361770	2	24	theme	cell	320:323	arg1	walls					325:329	the cell walls structure and composition	316:355	the cell walls structure and composition	316:355	In the cell walls in grass, the accessibility of structural polysaccharides is limited by the cell walls structure and composition mainly as a result of phenolic compounds.
31361770	4	25	from	emphasis	641:648	arg1	lignification					653:665	lignification	653:665	lignification	653:665	The developmental patterns observed in the internode cell walls in terms of its composition are reported with an emphasis on lignification, p-coumaroylation and feruloylation.
31361770	4	25	from	emphasis	641:648	arg1	feruloylation					689:701	feruloylation	689:701	feruloylation	689:701	The developmental patterns observed in the internode cell walls in terms of its composition are reported with an emphasis on lignification, p-coumaroylation and feruloylation.
31361770	4	25	from	emphasis	641:648	arg1	p-coumaroylation					668:683	p-coumaroylation	668:683	p-coumaroylation	668:683	The developmental patterns observed in the internode cell walls in terms of its composition are reported with an emphasis on lignification, p-coumaroylation and feruloylation.
31361770	2	26	theme	phenolic	379:386	arg1	compounds					388:396	phenolic compounds	379:396	phenolic compounds	379:396	In the cell walls in grass, the accessibility of structural polysaccharides is limited by the cell walls structure and composition mainly as a result of phenolic compounds.
31361770	6	27	theme	silage	991:996	arg1	maturity					998:1005	silage maturity	991:1005	silage maturity	991:1005	After flowering and until silage maturity, the slow deposition of secondary walls components occurs in the cortical region, and the deposited lignins are rich in β-O-4 bonds and are highly p-coumaroylated.
31361770	3	28	dep	walls	437:441	arg1	establishment					443:455	establishment	443:455	cell walls establishment	432:455	Here, we studied the patterns of cell walls establishment in the internode supporting the ear in three distinct maize genotypes.
31361770	1	29	theme	Plant	112:116	arg1	walls					123:127	Plant cell walls	112:127	Plant cell walls development	112:139	Plant cell walls development is an integrated process during which several components are deposited successively.
31361770	5	30	theme	rapid	857:861	arg1	deposition					863:872	the rapid deposition	853:872	the rapid deposition of secondary cell walls components and robust lignification in both the pith and the rind	853:962	We combined biochemical and histological approaches and revealed that internode cell walls development in maize before flowering is characterized by the rapid deposition of secondary cell walls components and robust lignification in both the pith and the rind.
31361770	0	31	theme	cell	11:14	arg1	walls					16:20	cell walls	11:20	cell walls	11:20	Changes in cell walls lignification, feruloylation and p-coumaroylation throughout maize internode development.
31361770	4	32	theme	internode	571:579	arg1	walls					586:590	the internode cell walls	567:590	the internode cell walls in terms of its composition	567:618	The developmental patterns observed in the internode cell walls in terms of its composition are reported with an emphasis on lignification, p-coumaroylation and feruloylation.
31361770	5	33	theme	biochemical	716:726	arg1	approaches					745:754	biochemical and histological approaches	716:754	biochemical and histological approaches	716:754	We combined biochemical and histological approaches and revealed that internode cell walls development in maize before flowering is characterized by the rapid deposition of secondary cell walls components and robust lignification in both the pith and the rind.
31361770	1	34	theme	cell	118:121	arg1	walls					123:127	Plant cell walls	112:127	Plant cell walls development	112:139	Plant cell walls development is an integrated process during which several components are deposited successively.
31361770	1	35	theme	integrated	147:156	arg1	process					158:164	an integrated process	144:164	an integrated process during which several components are deposited successively	144:223	Plant cell walls development is an integrated process during which several components are deposited successively.
31361770	1	35	theme	integrated	147:156	arg1	development					129:139	Plant cell walls development	112:139	Plant cell walls development	112:139	Plant cell walls development is an integrated process during which several components are deposited successively.
31361770	3	36	theme	cell	432:435	arg1	walls					437:441	cell walls establishment	432:455	cell walls establishment	432:455	Here, we studied the patterns of cell walls establishment in the internode supporting the ear in three distinct maize genotypes.
31361770	5	37	theme	histological	732:743	arg1	approaches					745:754	biochemical and histological approaches	716:754	biochemical and histological approaches	716:754	We combined biochemical and histological approaches and revealed that internode cell walls development in maize before flowering is characterized by the rapid deposition of secondary cell walls components and robust lignification in both the pith and the rind.
31361770	6	38	theme	slow	1012:1015	arg1	deposition					1017:1026	the slow deposition	1008:1026	the slow deposition of secondary walls components	1008:1056	After flowering and until silage maturity, the slow deposition of secondary walls components occurs in the cortical region, and the deposited lignins are rich in β-O-4 bonds and are highly p-coumaroylated.
31361770	5	39	theme	secondary	877:885	arg1	walls					892:896	secondary cell walls	877:896	secondary cell walls components and robust lignification	877:932	We combined biochemical and histological approaches and revealed that internode cell walls development in maize before flowering is characterized by the rapid deposition of secondary cell walls components and robust lignification in both the pith and the rind.
31361770	5	40	theme	robust	913:918	arg1	lignification					920:932	robust lignification	913:932	robust lignification	913:932	We combined biochemical and histological approaches and revealed that internode cell walls development in maize before flowering is characterized by the rapid deposition of secondary cell walls components and robust lignification in both the pith and the rind.
31361770	1	41	theme	walls	123:127	arg1	process					158:164	an integrated process	144:164	an integrated process during which several components are deposited successively	144:223	Plant cell walls development is an integrated process during which several components are deposited successively.
31361770	1	41	theme	walls	123:127	arg1	development					129:139	Plant cell walls development	112:139	Plant cell walls development	112:139	Plant cell walls development is an integrated process during which several components are deposited successively.
31361770	7	42	from	development	1302:1312	arg1	grass					1317:1321	grass	1317:1321	grass	1317:1321	We conclude the paper by proposing a revised spatiotemporal model based on that proposed by Terashima et al. (1993) for cell walls development in grass.
31361770	2	43	theme	cell	233:236	arg1	walls					238:242	the cell walls	229:242	the cell walls in grass	229:251	In the cell walls in grass, the accessibility of structural polysaccharides is limited by the cell walls structure and composition mainly as a result of phenolic compounds.
31361770	4	44	theme	developmental	532:544	arg1	patterns					546:553	The developmental patterns	528:553	The developmental patterns observed in the internode cell walls in terms of its composition	528:618	The developmental patterns observed in the internode cell walls in terms of its composition are reported with an emphasis on lignification, p-coumaroylation and feruloylation.
31361770	5	45	from	development	795:805	arg1	maize					810:814	maize	810:814	maize before flowering	810:831	We combined biochemical and histological approaches and revealed that internode cell walls development in maize before flowering is characterized by the rapid deposition of secondary cell walls components and robust lignification in both the pith and the rind.
31361770	5	46	from	deposition	863:872	arg1	pith					946:949	pith	946:949	pith	946:949	We combined biochemical and histological approaches and revealed that internode cell walls development in maize before flowering is characterized by the rapid deposition of secondary cell walls components and robust lignification in both the pith and the rind.
31361770	5	46	from	deposition	863:872	arg1	rind					959:962	rind	959:962	rind	959:962	We combined biochemical and histological approaches and revealed that internode cell walls development in maize before flowering is characterized by the rapid deposition of secondary cell walls components and robust lignification in both the pith and the rind.
31361770	2	47	dep	walls	325:329	arg1	composition					345:355	composition	345:355	composition	345:355	In the cell walls in grass, the accessibility of structural polysaccharides is limited by the cell walls structure and composition mainly as a result of phenolic compounds.
31361770	2	47	dep	walls	325:329	arg1	structure					331:339	structure	331:339	structure	331:339	In the cell walls in grass, the accessibility of structural polysaccharides is limited by the cell walls structure and composition mainly as a result of phenolic compounds.
31361770	2	47	dep	walls	325:329	arg1	walls					325:329	the cell walls structure and composition	316:355	the cell walls structure and composition	316:355	In the cell walls in grass, the accessibility of structural polysaccharides is limited by the cell walls structure and composition mainly as a result of phenolic compounds.
31361770	6	48	theme	components	1047:1056	arg1	deposition					1017:1026	the slow deposition	1008:1026	the slow deposition of secondary walls components	1008:1056	After flowering and until silage maturity, the slow deposition of secondary walls components occurs in the cortical region, and the deposited lignins are rich in β-O-4 bonds and are highly p-coumaroylated.
31361770	6	49	from	bonds	1133:1137	arg1	p-coumaroylated					1154:1168	p-coumaroylated	1154:1168	p-coumaroylated	1154:1168	After flowering and until silage maturity, the slow deposition of secondary walls components occurs in the cortical region, and the deposited lignins are rich in β-O-4 bonds and are highly p-coumaroylated.
31361770	6	49	from	bonds	1133:1137	arg1	rich					1119:1122	rich	1119:1122	rich	1119:1122	After flowering and until silage maturity, the slow deposition of secondary walls components occurs in the cortical region, and the deposited lignins are rich in β-O-4 bonds and are highly p-coumaroylated.
31361770	6	49	from	bonds	1133:1137	arg1	lignins					1107:1113	the deposited lignins	1093:1113	the deposited lignins	1093:1113	After flowering and until silage maturity, the slow deposition of secondary walls components occurs in the cortical region, and the deposited lignins are rich in β-O-4 bonds and are highly p-coumaroylated.
31361770	3	50	from	patterns	420:427	arg1	internode					464:472	the internode	460:472	the internode supporting the ear in three distinct maize genotypes	460:525	Here, we studied the patterns of cell walls establishment in the internode supporting the ear in three distinct maize genotypes.
31361770	6	51	theme	β-O-4	1127:1131	arg1	bonds					1133:1137	β-O-4 bonds	1127:1137	β-O-4 bonds	1127:1137	After flowering and until silage maturity, the slow deposition of secondary walls components occurs in the cortical region, and the deposited lignins are rich in β-O-4 bonds and are highly p-coumaroylated.
31361770	5	52	theme	internode	774:782	arg1	walls					789:793	internode cell walls	774:793	internode cell walls development in maize before flowering	774:831	We combined biochemical and histological approaches and revealed that internode cell walls development in maize before flowering is characterized by the rapid deposition of secondary cell walls components and robust lignification in both the pith and the rind.
31361770	6	53	theme	cortical	1072:1079	arg1	region					1081:1086	the cortical region	1068:1086	the cortical region	1068:1086	After flowering and until silage maturity, the slow deposition of secondary walls components occurs in the cortical region, and the deposited lignins are rich in β-O-4 bonds and are highly p-coumaroylated.
31361770	3	54	theme	maize	511:515	arg1	genotypes					517:525	three distinct maize genotypes	496:525	three distinct maize genotypes	496:525	Here, we studied the patterns of cell walls establishment in the internode supporting the ear in three distinct maize genotypes.
31361770	5	55	theme	walls	892:896	arg1	deposition					863:872	the rapid deposition	853:872	the rapid deposition of secondary cell walls components and robust lignification in both the pith and the rind	853:962	We combined biochemical and histological approaches and revealed that internode cell walls development in maize before flowering is characterized by the rapid deposition of secondary cell walls components and robust lignification in both the pith and the rind.
31361770	6	56	theme	secondary	1031:1039	arg1	components					1047:1056	secondary walls components	1031:1056	secondary walls components	1031:1056	After flowering and until silage maturity, the slow deposition of secondary walls components occurs in the cortical region, and the deposited lignins are rich in β-O-4 bonds and are highly p-coumaroylated.
31361770	5	57	theme	cell	784:787	arg1	walls					789:793	internode cell walls	774:793	internode cell walls development in maize before flowering	774:831	We combined biochemical and histological approaches and revealed that internode cell walls development in maize before flowering is characterized by the rapid deposition of secondary cell walls components and robust lignification in both the pith and the rind.
31361770	6	58	from	rich	1119:1122	arg1	bonds					1133:1137	β-O-4 bonds	1127:1137	β-O-4 bonds	1127:1137	After flowering and until silage maturity, the slow deposition of secondary walls components occurs in the cortical region, and the deposited lignins are rich in β-O-4 bonds and are highly p-coumaroylated.
31361770	4	59	theme	composition	608:618	arg1	terms					595:599	terms	595:599	terms of its composition	595:618	The developmental patterns observed in the internode cell walls in terms of its composition are reported with an emphasis on lignification, p-coumaroylation and feruloylation.
31330394	4	0	from	effects	612:618	arg1	roots					629:633	maize roots	623:633	maize roots	623:633	In a short-term (24 h) exposure, rosmarinic acid caused deleterious effects on maize roots, inhibiting the first enzymes of the phenylpropanoid pathway, phenylalanine ammonia-lyase and tyrosine ammonia-lyase, altering lignin composition and slightly increasing saccharification.
31330394	4	1	theme	pathway	688:694	arg1	enzymes					657:663	the first enzymes	647:663	the first enzymes of the phenylpropanoid pathway	647:694	In a short-term (24 h) exposure, rosmarinic acid caused deleterious effects on maize roots, inhibiting the first enzymes of the phenylpropanoid pathway, phenylalanine ammonia-lyase and tyrosine ammonia-lyase, altering lignin composition and slightly increasing saccharification.
31330394	4	1	theme	pathway	688:694	arg1	ammonia-lyase					711:723	phenylalanine ammonia-lyase	697:723	phenylalanine ammonia-lyase	697:723	In a short-term (24 h) exposure, rosmarinic acid caused deleterious effects on maize roots, inhibiting the first enzymes of the phenylpropanoid pathway, phenylalanine ammonia-lyase and tyrosine ammonia-lyase, altering lignin composition and slightly increasing saccharification.
31330394	4	1	theme	pathway	688:694	arg1	ammonia-lyase					738:750	tyrosine ammonia-lyase	729:750	tyrosine ammonia-lyase	729:750	In a short-term (24 h) exposure, rosmarinic acid caused deleterious effects on maize roots, inhibiting the first enzymes of the phenylpropanoid pathway, phenylalanine ammonia-lyase and tyrosine ammonia-lyase, altering lignin composition and slightly increasing saccharification.
31330394	6	2	theme	acid	1084:1087	arg1	application					1058:1068	exogenous application	1048:1068	exogenous application of rosmarinic acid on maize plants	1048:1103	This demonstrated that exogenous application of rosmarinic acid on maize plants improved saccharification, and represented an interesting approach in facilitating enzymatic hydrolysis of biomass polysaccharides and increasing bioethanol production.
31330394	1	3	theme	rosmarinic	175:184	arg1	acid					186:189	rosmarinic acid	175:189	rosmarinic acid	175:189	Biomimetically incorporated into the lignin structure, rosmarinic acid improves in vitro maize cell wall saccharification; however, no in planta studies have been performed.
31330394	1	4	theme	planta	258:263	arg1	studies					265:271	no in planta studies	252:271	no in planta studies	252:271	Biomimetically incorporated into the lignin structure, rosmarinic acid improves in vitro maize cell wall saccharification; however, no in planta studies have been performed.
31330394	5	5	dep	exposure	845:852	arg1	14 d					839:842	14 d	839:842	14 d	839:842	In a long-term (14 d) exposure, rosmarinic acid increased saccharification of maize stems by about 50% without any deleterious effects on plant growth, the phenylpropanoid pathway and lignin formation.
31330394	3	6	theme	maize	522:526	arg1	saccharification					502:517	saccharification	502:517	saccharification of maize	502:526	Its effects on growth, enzymes of the phenylpropanoid pathway, lignin, monomeric composition, and saccharification of maize were evaluated.
31330394	3	6	theme	maize	522:526	arg1	growth					419:424	growth	419:424	growth	419:424	Its effects on growth, enzymes of the phenylpropanoid pathway, lignin, monomeric composition, and saccharification of maize were evaluated.
31330394	3	6	theme	maize	522:526	arg1	composition					485:495	monomeric composition	475:495	monomeric composition	475:495	Its effects on growth, enzymes of the phenylpropanoid pathway, lignin, monomeric composition, and saccharification of maize were evaluated.
31330394	3	6	theme	maize	522:526	arg1	enzymes					427:433	enzymes	427:433	enzymes of the phenylpropanoid pathway	427:464	Its effects on growth, enzymes of the phenylpropanoid pathway, lignin, monomeric composition, and saccharification of maize were evaluated.
31330394	3	6	theme	maize	522:526	arg1	lignin					467:472	lignin	467:472	lignin	467:472	Its effects on growth, enzymes of the phenylpropanoid pathway, lignin, monomeric composition, and saccharification of maize were evaluated.
31330394	0	7	theme	maize	113:117	arg1	lignification					96:108	lignification	96:108	lignification	96:108	Exogenous application of rosmarinic acid improves saccharification without affecting growth and lignification of maize.
31330394	0	7	theme	maize	113:117	arg1	growth					85:90	growth	85:90	growth	85:90	Exogenous application of rosmarinic acid improves saccharification without affecting growth and lignification of maize.
31330394	5	8	from	effects	950:956	arg1	growth					967:972	plant growth	961:972	plant growth	961:972	In a long-term (14 d) exposure, rosmarinic acid increased saccharification of maize stems by about 50% without any deleterious effects on plant growth, the phenylpropanoid pathway and lignin formation.
31330394	5	8	from	effects	950:956	arg1	pathway					995:1001	the phenylpropanoid pathway	975:1001	the phenylpropanoid pathway	975:1001	In a long-term (14 d) exposure, rosmarinic acid increased saccharification of maize stems by about 50% without any deleterious effects on plant growth, the phenylpropanoid pathway and lignin formation.
31330394	5	8	from	effects	950:956	arg1	formation					1014:1022	lignin formation	1007:1022	lignin formation	1007:1022	In a long-term (14 d) exposure, rosmarinic acid increased saccharification of maize stems by about 50% without any deleterious effects on plant growth, the phenylpropanoid pathway and lignin formation.
31330394	6	9	from	application	1058:1068	arg1	plants					1098:1103	maize plants	1092:1103	maize plants	1092:1103	This demonstrated that exogenous application of rosmarinic acid on maize plants improved saccharification, and represented an interesting approach in facilitating enzymatic hydrolysis of biomass polysaccharides and increasing bioethanol production.
31330394	6	10	theme	bioethanol	1251:1260	arg1	production					1262:1271	bioethanol production	1251:1271	bioethanol production	1251:1271	This demonstrated that exogenous application of rosmarinic acid on maize plants improved saccharification, and represented an interesting approach in facilitating enzymatic hydrolysis of biomass polysaccharides and increasing bioethanol production.
31330394	6	11	theme	enzymatic	1188:1196	arg1	hydrolysis					1198:1207	enzymatic hydrolysis	1188:1207	enzymatic hydrolysis of biomass polysaccharides	1188:1234	This demonstrated that exogenous application of rosmarinic acid on maize plants improved saccharification, and represented an interesting approach in facilitating enzymatic hydrolysis of biomass polysaccharides and increasing bioethanol production.
31330394	4	12	dep	exposure	567:574	arg1	24 h					561:564	24 h	561:564	24 h	561:564	In a short-term (24 h) exposure, rosmarinic acid caused deleterious effects on maize roots, inhibiting the first enzymes of the phenylpropanoid pathway, phenylalanine ammonia-lyase and tyrosine ammonia-lyase, altering lignin composition and slightly increasing saccharification.
31330394	5	13	theme	phenylpropanoid	979:993	arg1	pathway					995:1001	the phenylpropanoid pathway	975:1001	the phenylpropanoid pathway	975:1001	In a long-term (14 d) exposure, rosmarinic acid increased saccharification of maize stems by about 50% without any deleterious effects on plant growth, the phenylpropanoid pathway and lignin formation.
31330394	4	14	theme	phenylalanine	697:709	arg1	enzymes					657:663	the first enzymes	647:663	the first enzymes of the phenylpropanoid pathway	647:694	In a short-term (24 h) exposure, rosmarinic acid caused deleterious effects on maize roots, inhibiting the first enzymes of the phenylpropanoid pathway, phenylalanine ammonia-lyase and tyrosine ammonia-lyase, altering lignin composition and slightly increasing saccharification.
31330394	4	14	theme	phenylalanine	697:709	arg1	ammonia-lyase					711:723	phenylalanine ammonia-lyase	697:723	phenylalanine ammonia-lyase	697:723	In a short-term (24 h) exposure, rosmarinic acid caused deleterious effects on maize roots, inhibiting the first enzymes of the phenylpropanoid pathway, phenylalanine ammonia-lyase and tyrosine ammonia-lyase, altering lignin composition and slightly increasing saccharification.
31330394	4	15	theme	tyrosine	729:736	arg1	enzymes					657:663	the first enzymes	647:663	the first enzymes of the phenylpropanoid pathway	647:694	In a short-term (24 h) exposure, rosmarinic acid caused deleterious effects on maize roots, inhibiting the first enzymes of the phenylpropanoid pathway, phenylalanine ammonia-lyase and tyrosine ammonia-lyase, altering lignin composition and slightly increasing saccharification.
31330394	4	15	theme	tyrosine	729:736	arg1	ammonia-lyase					738:750	tyrosine ammonia-lyase	729:750	tyrosine ammonia-lyase	729:750	In a short-term (24 h) exposure, rosmarinic acid caused deleterious effects on maize roots, inhibiting the first enzymes of the phenylpropanoid pathway, phenylalanine ammonia-lyase and tyrosine ammonia-lyase, altering lignin composition and slightly increasing saccharification.
31330394	5	16	theme	rosmarinic	855:864	arg1	acid					866:869	rosmarinic acid	855:869	rosmarinic acid	855:869	In a long-term (14 d) exposure, rosmarinic acid increased saccharification of maize stems by about 50% without any deleterious effects on plant growth, the phenylpropanoid pathway and lignin formation.
31330394	3	17	theme	monomeric	475:483	arg1	composition					485:495	monomeric composition	475:495	monomeric composition	475:495	Its effects on growth, enzymes of the phenylpropanoid pathway, lignin, monomeric composition, and saccharification of maize were evaluated.
31330394	3	18	theme	phenylpropanoid	442:456	arg1	pathway					458:464	the phenylpropanoid pathway	438:464	the phenylpropanoid pathway	438:464	Its effects on growth, enzymes of the phenylpropanoid pathway, lignin, monomeric composition, and saccharification of maize were evaluated.
31330394	4	19	theme	rosmarinic	577:586	arg1	acid					588:591	rosmarinic acid	577:591	rosmarinic acid	577:591	In a short-term (24 h) exposure, rosmarinic acid caused deleterious effects on maize roots, inhibiting the first enzymes of the phenylpropanoid pathway, phenylalanine ammonia-lyase and tyrosine ammonia-lyase, altering lignin composition and slightly increasing saccharification.
31330394	6	20	theme	polysaccharides	1220:1234	arg1	hydrolysis					1198:1207	enzymatic hydrolysis	1188:1207	enzymatic hydrolysis of biomass polysaccharides	1188:1234	This demonstrated that exogenous application of rosmarinic acid on maize plants improved saccharification, and represented an interesting approach in facilitating enzymatic hydrolysis of biomass polysaccharides and increasing bioethanol production.
31330394	1	21	theme	in	200:201	arg1	saccharification					225:240	in vitro maize cell wall saccharification	200:240	in vitro maize cell wall saccharification	200:240	Biomimetically incorporated into the lignin structure, rosmarinic acid improves in vitro maize cell wall saccharification; however, no in planta studies have been performed.
31330394	3	22	from	effects	408:414	arg1	lignin					467:472	lignin	467:472	lignin	467:472	Its effects on growth, enzymes of the phenylpropanoid pathway, lignin, monomeric composition, and saccharification of maize were evaluated.
31330394	3	22	from	effects	408:414	arg1	growth					419:424	growth	419:424	growth	419:424	Its effects on growth, enzymes of the phenylpropanoid pathway, lignin, monomeric composition, and saccharification of maize were evaluated.
31330394	3	22	from	effects	408:414	arg1	composition					485:495	monomeric composition	475:495	monomeric composition	475:495	Its effects on growth, enzymes of the phenylpropanoid pathway, lignin, monomeric composition, and saccharification of maize were evaluated.
31330394	3	22	from	effects	408:414	arg1	saccharification					502:517	saccharification	502:517	saccharification of maize	502:526	Its effects on growth, enzymes of the phenylpropanoid pathway, lignin, monomeric composition, and saccharification of maize were evaluated.
31330394	3	22	from	effects	408:414	arg1	enzymes					427:433	enzymes	427:433	enzymes of the phenylpropanoid pathway	427:464	Its effects on growth, enzymes of the phenylpropanoid pathway, lignin, monomeric composition, and saccharification of maize were evaluated.
31330394	4	23	theme	first	651:655	arg1	enzymes					657:663	the first enzymes	647:663	the first enzymes of the phenylpropanoid pathway	647:694	In a short-term (24 h) exposure, rosmarinic acid caused deleterious effects on maize roots, inhibiting the first enzymes of the phenylpropanoid pathway, phenylalanine ammonia-lyase and tyrosine ammonia-lyase, altering lignin composition and slightly increasing saccharification.
31330394	4	23	theme	first	651:655	arg1	ammonia-lyase					711:723	phenylalanine ammonia-lyase	697:723	phenylalanine ammonia-lyase	697:723	In a short-term (24 h) exposure, rosmarinic acid caused deleterious effects on maize roots, inhibiting the first enzymes of the phenylpropanoid pathway, phenylalanine ammonia-lyase and tyrosine ammonia-lyase, altering lignin composition and slightly increasing saccharification.
31330394	4	23	theme	first	651:655	arg1	ammonia-lyase					738:750	tyrosine ammonia-lyase	729:750	tyrosine ammonia-lyase	729:750	In a short-term (24 h) exposure, rosmarinic acid caused deleterious effects on maize roots, inhibiting the first enzymes of the phenylpropanoid pathway, phenylalanine ammonia-lyase and tyrosine ammonia-lyase, altering lignin composition and slightly increasing saccharification.
31330394	6	24	theme	biomass	1212:1218	arg1	polysaccharides					1220:1234	biomass polysaccharides	1212:1234	biomass polysaccharides	1212:1234	This demonstrated that exogenous application of rosmarinic acid on maize plants improved saccharification, and represented an interesting approach in facilitating enzymatic hydrolysis of biomass polysaccharides and increasing bioethanol production.
31330394	5	25	theme	maize	901:905	arg1	stems					907:911	maize stems	901:911	maize stems	901:911	In a long-term (14 d) exposure, rosmarinic acid increased saccharification of maize stems by about 50% without any deleterious effects on plant growth, the phenylpropanoid pathway and lignin formation.
31330394	0	26	theme	Exogenous	0:8	arg1	application					10:20	Exogenous application	0:20	Exogenous application of rosmarinic acid	0:39	Exogenous application of rosmarinic acid improves saccharification without affecting growth and lignification of maize.
31330394	5	27	theme	plant	961:965	arg1	growth					967:972	plant growth	961:972	plant growth	961:972	In a long-term (14 d) exposure, rosmarinic acid increased saccharification of maize stems by about 50% without any deleterious effects on plant growth, the phenylpropanoid pathway and lignin formation.
31330394	1	28	theme	maize	209:213	arg1	saccharification					225:240	in vitro maize cell wall saccharification	200:240	in vitro maize cell wall saccharification	200:240	Biomimetically incorporated into the lignin structure, rosmarinic acid improves in vitro maize cell wall saccharification; however, no in planta studies have been performed.
31330394	0	29	theme	rosmarinic	25:34	arg1	acid					36:39	rosmarinic acid	25:39	rosmarinic acid	25:39	Exogenous application of rosmarinic acid improves saccharification without affecting growth and lignification of maize.
31330394	2	30	theme	rosmarinic	315:324	arg1	itself					332:337	itself	332:337	itself	332:337	We hypothesized that rosmarinic acid, itself, could inducer saccharification without disturbing plant growth.
31330394	2	30	theme	rosmarinic	315:324	arg1	acid					326:329	rosmarinic acid	315:329	rosmarinic acid	315:329	We hypothesized that rosmarinic acid, itself, could inducer saccharification without disturbing plant growth.
31330394	1	31	theme	cell	215:218	arg1	saccharification					225:240	in vitro maize cell wall saccharification	200:240	in vitro maize cell wall saccharification	200:240	Biomimetically incorporated into the lignin structure, rosmarinic acid improves in vitro maize cell wall saccharification; however, no in planta studies have been performed.
31330394	6	32	theme	interesting	1151:1161	arg1	approach					1163:1170	an interesting approach	1148:1170	an interesting approach in facilitating enzymatic hydrolysis of biomass polysaccharides and increasing bioethanol production	1148:1271	This demonstrated that exogenous application of rosmarinic acid on maize plants improved saccharification, and represented an interesting approach in facilitating enzymatic hydrolysis of biomass polysaccharides and increasing bioethanol production.
31330394	4	33	dep	caused	593:598	arg1	inhibiting					636:645	inhibiting	636:645	inhibiting the first enzymes of the phenylpropanoid pathway, phenylalanine ammonia-lyase and tyrosine ammonia-lyase	636:750	In a short-term (24 h) exposure, rosmarinic acid caused deleterious effects on maize roots, inhibiting the first enzymes of the phenylpropanoid pathway, phenylalanine ammonia-lyase and tyrosine ammonia-lyase, altering lignin composition and slightly increasing saccharification.
31330394	4	33	dep	caused	593:598	arg1	increasing					794:803	increasing	794:803	slightly increasing saccharification	785:820	In a short-term (24 h) exposure, rosmarinic acid caused deleterious effects on maize roots, inhibiting the first enzymes of the phenylpropanoid pathway, phenylalanine ammonia-lyase and tyrosine ammonia-lyase, altering lignin composition and slightly increasing saccharification.
31330394	4	33	dep	caused	593:598	arg1	altering					753:760	altering	753:760	altering lignin composition	753:779	In a short-term (24 h) exposure, rosmarinic acid caused deleterious effects on maize roots, inhibiting the first enzymes of the phenylpropanoid pathway, phenylalanine ammonia-lyase and tyrosine ammonia-lyase, altering lignin composition and slightly increasing saccharification.
31330394	4	34	theme	lignin	762:767	arg1	composition					769:779	lignin composition	762:779	lignin composition	762:779	In a short-term (24 h) exposure, rosmarinic acid caused deleterious effects on maize roots, inhibiting the first enzymes of the phenylpropanoid pathway, phenylalanine ammonia-lyase and tyrosine ammonia-lyase, altering lignin composition and slightly increasing saccharification.
31330394	1	35	theme	wall	220:223	arg1	saccharification					225:240	in vitro maize cell wall saccharification	200:240	in vitro maize cell wall saccharification	200:240	Biomimetically incorporated into the lignin structure, rosmarinic acid improves in vitro maize cell wall saccharification; however, no in planta studies have been performed.
31330394	5	36	theme	stems	907:911	arg1	saccharification					881:896	saccharification	881:896	saccharification of maize stems	881:911	In a long-term (14 d) exposure, rosmarinic acid increased saccharification of maize stems by about 50% without any deleterious effects on plant growth, the phenylpropanoid pathway and lignin formation.
31330394	6	37	theme	rosmarinic	1073:1082	arg1	acid					1084:1087	rosmarinic acid	1073:1087	rosmarinic acid	1073:1087	This demonstrated that exogenous application of rosmarinic acid on maize plants improved saccharification, and represented an interesting approach in facilitating enzymatic hydrolysis of biomass polysaccharides and increasing bioethanol production.
31330394	0	38	theme	acid	36:39	arg1	application					10:20	Exogenous application	0:20	Exogenous application of rosmarinic acid	0:39	Exogenous application of rosmarinic acid improves saccharification without affecting growth and lignification of maize.
31330394	2	39	theme	inducer	346:352	arg1	saccharification					354:369	inducer saccharification	346:369	inducer saccharification	346:369	We hypothesized that rosmarinic acid, itself, could inducer saccharification without disturbing plant growth.
31330394	5	40	theme	long-term	828:836	arg1	exposure					845:852	a long-term (14 d) exposure	826:852	a long-term (14 d) exposure	826:852	In a long-term (14 d) exposure, rosmarinic acid increased saccharification of maize stems by about 50% without any deleterious effects on plant growth, the phenylpropanoid pathway and lignin formation.
31330394	4	41	theme	short-term	549:558	arg1	exposure					567:574	a short-term (24 h) exposure	547:574	a short-term (24 h) exposure	547:574	In a short-term (24 h) exposure, rosmarinic acid caused deleterious effects on maize roots, inhibiting the first enzymes of the phenylpropanoid pathway, phenylalanine ammonia-lyase and tyrosine ammonia-lyase, altering lignin composition and slightly increasing saccharification.
31330394	1	42	dep	in	200:201	arg1	vitro					203:207	vitro	203:207	vitro	203:207	Biomimetically incorporated into the lignin structure, rosmarinic acid improves in vitro maize cell wall saccharification; however, no in planta studies have been performed.
31330394	5	43	theme	lignin	1007:1012	arg1	formation					1014:1022	lignin formation	1007:1022	lignin formation	1007:1022	In a long-term (14 d) exposure, rosmarinic acid increased saccharification of maize stems by about 50% without any deleterious effects on plant growth, the phenylpropanoid pathway and lignin formation.
31330394	6	44	theme	exogenous	1048:1056	arg1	application					1058:1068	exogenous application	1048:1068	exogenous application of rosmarinic acid on maize plants	1048:1103	This demonstrated that exogenous application of rosmarinic acid on maize plants improved saccharification, and represented an interesting approach in facilitating enzymatic hydrolysis of biomass polysaccharides and increasing bioethanol production.
31330394	5	45	theme	deleterious	938:948	arg1	effects					950:956	any deleterious effects	934:956	any deleterious effects on plant growth, the phenylpropanoid pathway and lignin formation	934:1022	In a long-term (14 d) exposure, rosmarinic acid increased saccharification of maize stems by about 50% without any deleterious effects on plant growth, the phenylpropanoid pathway and lignin formation.
31330394	6	46	theme	maize	1092:1096	arg1	plants					1098:1103	maize plants	1092:1103	maize plants	1092:1103	This demonstrated that exogenous application of rosmarinic acid on maize plants improved saccharification, and represented an interesting approach in facilitating enzymatic hydrolysis of biomass polysaccharides and increasing bioethanol production.
31330394	1	47	theme	lignin	157:162	arg1	structure					164:172	the lignin structure	153:172	the lignin structure	153:172	Biomimetically incorporated into the lignin structure, rosmarinic acid improves in vitro maize cell wall saccharification; however, no in planta studies have been performed.
31330394	4	48	theme	deleterious	600:610	arg1	effects					612:618	deleterious effects	600:618	deleterious effects on maize roots	600:633	In a short-term (24 h) exposure, rosmarinic acid caused deleterious effects on maize roots, inhibiting the first enzymes of the phenylpropanoid pathway, phenylalanine ammonia-lyase and tyrosine ammonia-lyase, altering lignin composition and slightly increasing saccharification.
31330394	2	49	theme	plant	390:394	arg1	growth					396:401	plant growth	390:401	plant growth	390:401	We hypothesized that rosmarinic acid, itself, could inducer saccharification without disturbing plant growth.
31330394	3	50	theme	pathway	458:464	arg1	saccharification					502:517	saccharification	502:517	saccharification of maize	502:526	Its effects on growth, enzymes of the phenylpropanoid pathway, lignin, monomeric composition, and saccharification of maize were evaluated.
31330394	3	50	theme	pathway	458:464	arg1	growth					419:424	growth	419:424	growth	419:424	Its effects on growth, enzymes of the phenylpropanoid pathway, lignin, monomeric composition, and saccharification of maize were evaluated.
31330394	3	50	theme	pathway	458:464	arg1	composition					485:495	monomeric composition	475:495	monomeric composition	475:495	Its effects on growth, enzymes of the phenylpropanoid pathway, lignin, monomeric composition, and saccharification of maize were evaluated.
31330394	3	50	theme	pathway	458:464	arg1	enzymes					427:433	enzymes	427:433	enzymes of the phenylpropanoid pathway	427:464	Its effects on growth, enzymes of the phenylpropanoid pathway, lignin, monomeric composition, and saccharification of maize were evaluated.
31330394	3	50	theme	pathway	458:464	arg1	lignin					467:472	lignin	467:472	lignin	467:472	Its effects on growth, enzymes of the phenylpropanoid pathway, lignin, monomeric composition, and saccharification of maize were evaluated.
31330394	4	51	theme	maize	623:627	arg1	roots					629:633	maize roots	623:633	maize roots	623:633	In a short-term (24 h) exposure, rosmarinic acid caused deleterious effects on maize roots, inhibiting the first enzymes of the phenylpropanoid pathway, phenylalanine ammonia-lyase and tyrosine ammonia-lyase, altering lignin composition and slightly increasing saccharification.
31330394	4	52	theme	phenylpropanoid	672:686	arg1	pathway					688:694	the phenylpropanoid pathway	668:694	the phenylpropanoid pathway	668:694	In a short-term (24 h) exposure, rosmarinic acid caused deleterious effects on maize roots, inhibiting the first enzymes of the phenylpropanoid pathway, phenylalanine ammonia-lyase and tyrosine ammonia-lyase, altering lignin composition and slightly increasing saccharification.
31330394	1	53	theme	in	255:256	arg1	studies					265:271	no in planta studies	252:271	no in planta studies	252:271	Biomimetically incorporated into the lignin structure, rosmarinic acid improves in vitro maize cell wall saccharification; however, no in planta studies have been performed.
30073560	2	0	theme	feeding	511:517	arg1	habits					519:524	host feeding habits	506:524	host feeding habits	506:524	To the best of our knowledge, the fundamental question of how the abundance and function of the gut microbiota of domestic dogs evolved to adapt to the changes in host feeding habits has yet to be addressed.
30073560	4	1	theme	wolves	924:929	arg1	diets					931:935	wolves diets	924:935	wolves diets	924:935	Furthermore, a number of taxa observed in higher numbers in domestic dogs are related to carbohydrate metabolism, which may be because that there were more complicated polysaccharides in dogs diets than that in wolves diets.
30073560	3	2	theme	comparative	570:580	arg1	analyses					582:589	our comparative analyses	566:589	our comparative analyses of gut metagenomes	566:608	In this study, our comparative analyses of gut metagenomes showed that the abundance of gut microbiota between the two species have some significant differences.
30073560	0	3	theme	gray	157:160	arg1	wolves					162:167	gray wolves	157:167	gray wolves (Canis lupus)	157:181	Changes in feeding habits promoted the differentiation of the composition and function of gut microbiotas between domestic dogs (Canis lupus familiaris) and gray wolves (Canis lupus).
30073560	0	3	theme	gray	157:160	arg1	lupus					176:180	Canis lupus	170:180	Canis lupus	170:180	Changes in feeding habits promoted the differentiation of the composition and function of gut microbiotas between domestic dogs (Canis lupus familiaris) and gray wolves (Canis lupus).
30073560	4	4	theme	higher	755:760	arg1	numbers					762:768	higher numbers	755:768	higher numbers in domestic dogs	755:785	Furthermore, a number of taxa observed in higher numbers in domestic dogs are related to carbohydrate metabolism, which may be because that there were more complicated polysaccharides in dogs diets than that in wolves diets.
30073560	4	5	located	observed	743:750	arg1	numbers					762:768	higher numbers	755:768	higher numbers in domestic dogs	755:785	Furthermore, a number of taxa observed in higher numbers in domestic dogs are related to carbohydrate metabolism, which may be because that there were more complicated polysaccharides in dogs diets than that in wolves diets.
30073560	4	5	located	observed	743:750	arg2	taxa					738:741	taxa	738:741	taxa observed in higher numbers in domestic dogs	738:785	Furthermore, a number of taxa observed in higher numbers in domestic dogs are related to carbohydrate metabolism, which may be because that there were more complicated polysaccharides in dogs diets than that in wolves diets.
30073560	3	6	theme	metagenomes	598:608	arg1	analyses					582:589	our comparative analyses	566:589	our comparative analyses of gut metagenomes	566:608	In this study, our comparative analyses of gut metagenomes showed that the abundance of gut microbiota between the two species have some significant differences.
30073560	8	7	theme	dogs	1719:1722	arg1	ability					1699:1705	the ability	1695:1705	the ability of domestic dogs to adapt to changes in food composition	1695:1762	Our results indicate that the function and abundance of gut microbiota of domestic dogs has been adapted to domestication, which is of great significance for the ability of domestic dogs to adapt to changes in food composition.
30073560	5	8	theme	module	1057:1062	arg1	family					1064:1069	carbohydrate-binding module family 25 (CBM25)	1036:1080	carbohydrate-binding module family 25 (CBM25)	1036:1080	A significant difference in the abundance of genes encoding glycosyltransferase family 34 (GT34), carbohydrate-binding module family 25 (CBM25), and glycoside hydrolase family 13 (GH13) between the gut microbiota metagenomes of domestic dogs and gray wolves also supported this observation.
30073560	3	9	theme	gut	639:641	arg1	microbiota					643:652	gut microbiota	639:652	gut microbiota	639:652	In this study, our comparative analyses of gut metagenomes showed that the abundance of gut microbiota between the two species have some significant differences.
30073560	7	10	theme	dietary	1497:1503	arg1	composition					1505:1515	the dietary composition	1493:1515	the dietary composition of wolves and dogs	1493:1534	This result showed that compared with wolves, the domestic dog diet contains a smaller amount of animal protein, which is consistent with the dietary composition of wolves and dogs.
30073560	4	11	theme	carbohydrate	802:813	arg1	metabolism					815:824	carbohydrate metabolism	802:824	carbohydrate metabolism	802:824	Furthermore, a number of taxa observed in higher numbers in domestic dogs are related to carbohydrate metabolism, which may be because that there were more complicated polysaccharides in dogs diets than that in wolves diets.
30073560	0	12	theme	domestic	114:121	arg1	familiaris					141:150	Canis lupus familiaris	129:150	Canis lupus familiaris	129:150	Changes in feeding habits promoted the differentiation of the composition and function of gut microbiotas between domestic dogs (Canis lupus familiaris) and gray wolves (Canis lupus).
30073560	0	12	theme	domestic	114:121	arg1	dogs					123:126	domestic dogs	114:126	domestic dogs (Canis lupus familiaris)	114:151	Changes in feeding habits promoted the differentiation of the composition and function of gut microbiotas between domestic dogs (Canis lupus familiaris) and gray wolves (Canis lupus).
30073560	5	13	theme	significant	940:950	arg1	difference					952:961	A significant difference	938:961	A significant difference in the abundance of genes encoding glycosyltransferase family 34 (GT34), carbohydrate-binding module family 25 (CBM25), and glycoside hydrolase family 13 (GH13) between the gut microbiota metagenomes of domestic dogs and gray wolves	938:1194	A significant difference in the abundance of genes encoding glycosyltransferase family 34 (GT34), carbohydrate-binding module family 25 (CBM25), and glycoside hydrolase family 13 (GH13) between the gut microbiota metagenomes of domestic dogs and gray wolves also supported this observation.
30073560	8	14	theme	gut	1593:1595	arg1	microbiota					1597:1606	gut microbiota	1593:1606	gut microbiota of domestic dogs	1593:1623	Our results indicate that the function and abundance of gut microbiota of domestic dogs has been adapted to domestication, which is of great significance for the ability of domestic dogs to adapt to changes in food composition.
30073560	2	15	theme	domestic	457:464	arg1	dogs					466:469	domestic dogs	457:469	domestic dogs	457:469	To the best of our knowledge, the fundamental question of how the abundance and function of the gut microbiota of domestic dogs evolved to adapt to the changes in host feeding habits has yet to be addressed.
30073560	8	16	theme	food	1747:1750	arg1	composition					1752:1762	food composition	1747:1762	food composition	1747:1762	Our results indicate that the function and abundance of gut microbiota of domestic dogs has been adapted to domestication, which is of great significance for the ability of domestic dogs to adapt to changes in food composition.
30073560	0	17	theme	lupus	135:139	arg1	familiaris					141:150	Canis lupus familiaris	129:150	Canis lupus familiaris	129:150	Changes in feeding habits promoted the differentiation of the composition and function of gut microbiotas between domestic dogs (Canis lupus familiaris) and gray wolves (Canis lupus).
30073560	0	17	theme	lupus	135:139	arg1	dogs					123:126	domestic dogs	114:126	domestic dogs (Canis lupus familiaris)	114:151	Changes in feeding habits promoted the differentiation of the composition and function of gut microbiotas between domestic dogs (Canis lupus familiaris) and gray wolves (Canis lupus).
30073560	8	18	theme	dogs	1620:1623	arg1	microbiota					1597:1606	gut microbiota	1593:1606	gut microbiota of domestic dogs	1593:1623	Our results indicate that the function and abundance of gut microbiota of domestic dogs has been adapted to domestication, which is of great significance for the ability of domestic dogs to adapt to changes in food composition.
30073560	8	19	theme	domestic	1710:1717	arg1	dogs					1719:1722	domestic dogs	1710:1722	domestic dogs	1710:1722	Our results indicate that the function and abundance of gut microbiota of domestic dogs has been adapted to domestication, which is of great significance for the ability of domestic dogs to adapt to changes in food composition.
30073560	5	20	theme	gut	1136:1138	arg1	metagenomes					1151:1161	the gut microbiota metagenomes	1132:1161	the gut microbiota metagenomes of domestic dogs and gray wolves	1132:1194	A significant difference in the abundance of genes encoding glycosyltransferase family 34 (GT34), carbohydrate-binding module family 25 (CBM25), and glycoside hydrolase family 13 (GH13) between the gut microbiota metagenomes of domestic dogs and gray wolves also supported this observation.
30073560	3	21	theme	significant	688:698	arg1	differences					700:710	some significant differences	683:710	some significant differences	683:710	In this study, our comparative analyses of gut metagenomes showed that the abundance of gut microbiota between the two species have some significant differences.
30073560	7	22	theme	animal	1452:1457	arg1	protein					1459:1465	animal protein	1452:1465	animal protein	1452:1465	This result showed that compared with wolves, the domestic dog diet contains a smaller amount of animal protein, which is consistent with the dietary composition of wolves and dogs.
30073560	6	23	theme	domestic	1246:1253	arg1	microbiota					1263:1272	the domestic dog gut microbiota	1242:1272	the domestic dog gut microbiota	1242:1272	Furthermore, the domestic dog gut microbiota has greater valine, leucine and isoleucine biosynthesis and nitrogen metabolism.
30073560	5	24	theme	glycosyltransferase	998:1016	arg1	family					1018:1023	glycosyltransferase family 34	998:1026	glycosyltransferase family 34 (GT34)	998:1033	A significant difference in the abundance of genes encoding glycosyltransferase family 34 (GT34), carbohydrate-binding module family 25 (CBM25), and glycoside hydrolase family 13 (GH13) between the gut microbiota metagenomes of domestic dogs and gray wolves also supported this observation.
30073560	5	24	theme	glycosyltransferase	998:1016	arg1	GT34					1029:1032	GT34	1029:1032	GT34	1029:1032	A significant difference in the abundance of genes encoding glycosyltransferase family 34 (GT34), carbohydrate-binding module family 25 (CBM25), and glycoside hydrolase family 13 (GH13) between the gut microbiota metagenomes of domestic dogs and gray wolves also supported this observation.
30073560	6	25	theme	valine	1286:1291	arg1	biosynthesis					1317:1328	greater valine, leucine and isoleucine biosynthesis	1278:1328	greater valine, leucine and isoleucine biosynthesis	1278:1328	Furthermore, the domestic dog gut microbiota has greater valine, leucine and isoleucine biosynthesis and nitrogen metabolism.
30073560	2	26	theme	microbiota	443:452	arg1	function					423:430	function	423:430	function	423:430	To the best of our knowledge, the fundamental question of how the abundance and function of the gut microbiota of domestic dogs evolved to adapt to the changes in host feeding habits has yet to be addressed.
30073560	2	26	theme	microbiota	443:452	arg1	abundance					409:417	abundance	409:417	abundance	409:417	To the best of our knowledge, the fundamental question of how the abundance and function of the gut microbiota of domestic dogs evolved to adapt to the changes in host feeding habits has yet to be addressed.
30073560	0	27	from	Changes	0:6	arg1	habits					19:24	feeding habits	11:24	feeding habits	11:24	Changes in feeding habits promoted the differentiation of the composition and function of gut microbiotas between domestic dogs (Canis lupus familiaris) and gray wolves (Canis lupus).
30073560	5	28	theme	wolves	1189:1194	arg1	metagenomes					1151:1161	the gut microbiota metagenomes	1132:1161	the gut microbiota metagenomes of domestic dogs and gray wolves	1132:1194	A significant difference in the abundance of genes encoding glycosyltransferase family 34 (GT34), carbohydrate-binding module family 25 (CBM25), and glycoside hydrolase family 13 (GH13) between the gut microbiota metagenomes of domestic dogs and gray wolves also supported this observation.
30073560	0	29	theme	microbiotas	94:104	arg1	function					78:85	function	78:85	function	78:85	Changes in feeding habits promoted the differentiation of the composition and function of gut microbiotas between domestic dogs (Canis lupus familiaris) and gray wolves (Canis lupus).
30073560	0	29	theme	microbiotas	94:104	arg1	composition					62:72	composition	62:72	composition	62:72	Changes in feeding habits promoted the differentiation of the composition and function of gut microbiotas between domestic dogs (Canis lupus familiaris) and gray wolves (Canis lupus).
30073560	5	30	theme	genes	983:987	arg1	abundance					970:978	the abundance	966:978	the abundance of genes encoding glycosyltransferase family 34 (GT34), carbohydrate-binding module family 25 (CBM25), and glycoside hydrolase family 13 (GH13)	966:1122	A significant difference in the abundance of genes encoding glycosyltransferase family 34 (GT34), carbohydrate-binding module family 25 (CBM25), and glycoside hydrolase family 13 (GH13) between the gut microbiota metagenomes of domestic dogs and gray wolves also supported this observation.
30073560	7	31	with	consistent	1477:1486	arg1	composition					1505:1515	the dietary composition	1493:1515	the dietary composition of wolves and dogs	1493:1534	This result showed that compared with wolves, the domestic dog diet contains a smaller amount of animal protein, which is consistent with the dietary composition of wolves and dogs.
30073560	7	32	theme	dogs	1531:1534	arg1	composition					1505:1515	the dietary composition	1493:1515	the dietary composition of wolves and dogs	1493:1534	This result showed that compared with wolves, the domestic dog diet contains a smaller amount of animal protein, which is consistent with the dietary composition of wolves and dogs.
30073560	0	33	theme	composition	62:72	arg1	differentiation					39:53	the differentiation	35:53	the differentiation of the composition and function of gut microbiotas between domestic dogs (Canis lupus familiaris) and gray wolves (Canis lupus)	35:181	Changes in feeding habits promoted the differentiation of the composition and function of gut microbiotas between domestic dogs (Canis lupus familiaris) and gray wolves (Canis lupus).
30073560	6	34	theme	leucine	1294:1300	arg1	biosynthesis					1317:1328	greater valine, leucine and isoleucine biosynthesis	1278:1328	greater valine, leucine and isoleucine biosynthesis	1278:1328	Furthermore, the domestic dog gut microbiota has greater valine, leucine and isoleucine biosynthesis and nitrogen metabolism.
30073560	1	35	theme	close	232:236	arg1	relatives					238:246	their domesticated and close relatives	209:246	their domesticated and close relatives	209:246	Wolves (Canis lupus) and their domesticated and close relatives, dogs (Canis lupus familiaris), have great differences in their diets and living environments.
30073560	7	36	theme	wolves	1520:1525	arg1	composition					1505:1515	the dietary composition	1493:1515	the dietary composition of wolves and dogs	1493:1534	This result showed that compared with wolves, the domestic dog diet contains a smaller amount of animal protein, which is consistent with the dietary composition of wolves and dogs.
30073560	2	37	from	changes	495:501	arg1	habits					519:524	host feeding habits	506:524	host feeding habits	506:524	To the best of our knowledge, the fundamental question of how the abundance and function of the gut microbiota of domestic dogs evolved to adapt to the changes in host feeding habits has yet to be addressed.
30073560	5	38	theme	domestic	1166:1173	arg1	dogs					1175:1178	domestic dogs	1166:1178	domestic dogs	1166:1178	A significant difference in the abundance of genes encoding glycosyltransferase family 34 (GT34), carbohydrate-binding module family 25 (CBM25), and glycoside hydrolase family 13 (GH13) between the gut microbiota metagenomes of domestic dogs and gray wolves also supported this observation.
30073560	0	39	theme	feeding	11:17	arg1	habits					19:24	feeding habits	11:24	feeding habits	11:24	Changes in feeding habits promoted the differentiation of the composition and function of gut microbiotas between domestic dogs (Canis lupus familiaris) and gray wolves (Canis lupus).
30073560	6	40	theme	gut	1259:1261	arg1	microbiota					1263:1272	the domestic dog gut microbiota	1242:1272	the domestic dog gut microbiota	1242:1272	Furthermore, the domestic dog gut microbiota has greater valine, leucine and isoleucine biosynthesis and nitrogen metabolism.
30073560	1	41	theme	lupus	261:265	arg1	dogs					249:252	dogs	249:252	dogs (Canis lupus familiaris)	249:277	Wolves (Canis lupus) and their domesticated and close relatives, dogs (Canis lupus familiaris), have great differences in their diets and living environments.
30073560	1	41	theme	lupus	261:265	arg1	familiaris					267:276	Canis lupus familiaris	255:276	Canis lupus familiaris	255:276	Wolves (Canis lupus) and their domesticated and close relatives, dogs (Canis lupus familiaris), have great differences in their diets and living environments.
30073560	7	42	contain	contains	1423:1430	arg2	protein					1459:1465	animal protein	1452:1465	animal protein	1452:1465	This result showed that compared with wolves, the domestic dog diet contains a smaller amount of animal protein, which is consistent with the dietary composition of wolves and dogs.
30073560	7	42	contain	contains	1423:1430	arg2	amount					1442:1447	a smaller amount	1432:1447	a smaller amount	1432:1447	This result showed that compared with wolves, the domestic dog diet contains a smaller amount of animal protein, which is consistent with the dietary composition of wolves and dogs.
30073560	7	42	contain	contains	1423:1430	arg1	diet					1418:1421	the domestic dog diet	1401:1421	the domestic dog diet	1401:1421	This result showed that compared with wolves, the domestic dog diet contains a smaller amount of animal protein, which is consistent with the dietary composition of wolves and dogs.
30073560	7	42	contain	contains	1423:1430	arg2	consistent					1477:1486	consistent	1477:1486	consistent	1477:1486	This result showed that compared with wolves, the domestic dog diet contains a smaller amount of animal protein, which is consistent with the dietary composition of wolves and dogs.
30073560	5	43	theme	glycoside	1087:1095	arg1	family					1107:1112	glycoside hydrolase family 13 (GH13)	1087:1122	glycoside hydrolase family 13 (GH13)	1087:1122	A significant difference in the abundance of genes encoding glycosyltransferase family 34 (GT34), carbohydrate-binding module family 25 (CBM25), and glycoside hydrolase family 13 (GH13) between the gut microbiota metagenomes of domestic dogs and gray wolves also supported this observation.
30073560	6	44	contain	has	1274:1276	arg1	microbiota					1263:1272	the domestic dog gut microbiota	1242:1272	the domestic dog gut microbiota	1242:1272	Furthermore, the domestic dog gut microbiota has greater valine, leucine and isoleucine biosynthesis and nitrogen metabolism.
30073560	6	44	contain	has	1274:1276	arg2	biosynthesis					1317:1328	greater valine, leucine and isoleucine biosynthesis	1278:1328	greater valine, leucine and isoleucine biosynthesis	1278:1328	Furthermore, the domestic dog gut microbiota has greater valine, leucine and isoleucine biosynthesis and nitrogen metabolism.
30073560	6	44	contain	has	1274:1276	arg2	metabolism					1343:1352	nitrogen metabolism	1334:1352	nitrogen metabolism	1334:1352	Furthermore, the domestic dog gut microbiota has greater valine, leucine and isoleucine biosynthesis and nitrogen metabolism.
30073560	8	45	from	changes	1736:1742	arg1	composition					1752:1762	food composition	1747:1762	food composition	1747:1762	Our results indicate that the function and abundance of gut microbiota of domestic dogs has been adapted to domestication, which is of great significance for the ability of domestic dogs to adapt to changes in food composition.
30073560	3	46	theme	gut	594:596	arg1	metagenomes					598:608	gut metagenomes	594:608	gut metagenomes	594:608	In this study, our comparative analyses of gut metagenomes showed that the abundance of gut microbiota between the two species have some significant differences.
30073560	2	47	theme	host	506:509	arg1	habits					519:524	host feeding habits	506:524	host feeding habits	506:524	To the best of our knowledge, the fundamental question of how the abundance and function of the gut microbiota of domestic dogs evolved to adapt to the changes in host feeding habits has yet to be addressed.
30073560	0	48	theme	Canis	170:174	arg1	wolves					162:167	gray wolves	157:167	gray wolves (Canis lupus)	157:181	Changes in feeding habits promoted the differentiation of the composition and function of gut microbiotas between domestic dogs (Canis lupus familiaris) and gray wolves (Canis lupus).
30073560	0	48	theme	Canis	170:174	arg1	lupus					176:180	Canis lupus	170:180	Canis lupus	170:180	Changes in feeding habits promoted the differentiation of the composition and function of gut microbiotas between domestic dogs (Canis lupus familiaris) and gray wolves (Canis lupus).
30073560	6	49	theme	isoleucine	1306:1315	arg1	biosynthesis					1317:1328	greater valine, leucine and isoleucine biosynthesis	1278:1328	greater valine, leucine and isoleucine biosynthesis	1278:1328	Furthermore, the domestic dog gut microbiota has greater valine, leucine and isoleucine biosynthesis and nitrogen metabolism.
30073560	1	50	theme	domesticated	215:226	arg1	relatives					238:246	their domesticated and close relatives	209:246	their domesticated and close relatives	209:246	Wolves (Canis lupus) and their domesticated and close relatives, dogs (Canis lupus familiaris), have great differences in their diets and living environments.
30073560	4	51	theme	taxa	738:741	arg1	number					728:733	a number	726:733	a number of taxa observed in higher numbers in domestic dogs	726:785	Furthermore, a number of taxa observed in higher numbers in domestic dogs are related to carbohydrate metabolism, which may be because that there were more complicated polysaccharides in dogs diets than that in wolves diets.
30073560	4	51	theme	taxa	738:741	arg1	related					791:797	related	791:797	related	791:797	Furthermore, a number of taxa observed in higher numbers in domestic dogs are related to carbohydrate metabolism, which may be because that there were more complicated polysaccharides in dogs diets than that in wolves diets.
30073560	3	52	theme	microbiota	643:652	arg1	abundance					626:634	the abundance	622:634	the abundance of gut microbiota between the two species	622:676	In this study, our comparative analyses of gut metagenomes showed that the abundance of gut microbiota between the two species have some significant differences.
30073560	2	53	theme	dogs	466:469	arg1	function					423:430	function	423:430	function	423:430	To the best of our knowledge, the fundamental question of how the abundance and function of the gut microbiota of domestic dogs evolved to adapt to the changes in host feeding habits has yet to be addressed.
30073560	2	53	theme	dogs	466:469	arg1	abundance					409:417	abundance	409:417	abundance	409:417	To the best of our knowledge, the fundamental question of how the abundance and function of the gut microbiota of domestic dogs evolved to adapt to the changes in host feeding habits has yet to be addressed.
30073560	0	54	theme	Canis	129:133	arg1	familiaris					141:150	Canis lupus familiaris	129:150	Canis lupus familiaris	129:150	Changes in feeding habits promoted the differentiation of the composition and function of gut microbiotas between domestic dogs (Canis lupus familiaris) and gray wolves (Canis lupus).
30073560	0	54	theme	Canis	129:133	arg1	dogs					123:126	domestic dogs	114:126	domestic dogs (Canis lupus familiaris)	114:151	Changes in feeding habits promoted the differentiation of the composition and function of gut microbiotas between domestic dogs (Canis lupus familiaris) and gray wolves (Canis lupus).
30073560	1	55	theme	living	322:327	arg1	environments					329:340	living environments	322:340	living environments	322:340	Wolves (Canis lupus) and their domesticated and close relatives, dogs (Canis lupus familiaris), have great differences in their diets and living environments.
30073560	8	56	theme	microbiota	1597:1606	arg1	abundance					1580:1588	abundance	1580:1588	abundance	1580:1588	Our results indicate that the function and abundance of gut microbiota of domestic dogs has been adapted to domestication, which is of great significance for the ability of domestic dogs to adapt to changes in food composition.
30073560	8	56	theme	microbiota	1597:1606	arg1	function					1567:1574	function	1567:1574	function	1567:1574	Our results indicate that the function and abundance of gut microbiota of domestic dogs has been adapted to domestication, which is of great significance for the ability of domestic dogs to adapt to changes in food composition.
30073560	7	57	theme	protein	1459:1465	arg1	amount					1442:1447	a smaller amount	1432:1447	a smaller amount	1432:1447	This result showed that compared with wolves, the domestic dog diet contains a smaller amount of animal protein, which is consistent with the dietary composition of wolves and dogs.
30073560	7	57	theme	protein	1459:1465	arg1	protein					1459:1465	animal protein	1452:1465	animal protein	1452:1465	This result showed that compared with wolves, the domestic dog diet contains a smaller amount of animal protein, which is consistent with the dietary composition of wolves and dogs.
30073560	7	57	theme	protein	1459:1465	arg1	consistent					1477:1486	consistent	1477:1486	consistent	1477:1486	This result showed that compared with wolves, the domestic dog diet contains a smaller amount of animal protein, which is consistent with the dietary composition of wolves and dogs.
30073560	4	58	theme	domestic	773:780	arg1	dogs					782:785	domestic dogs	773:785	domestic dogs	773:785	Furthermore, a number of taxa observed in higher numbers in domestic dogs are related to carbohydrate metabolism, which may be because that there were more complicated polysaccharides in dogs diets than that in wolves diets.
30073560	8	59	theme	domestic	1611:1618	arg1	dogs					1620:1623	domestic dogs	1611:1623	domestic dogs	1611:1623	Our results indicate that the function and abundance of gut microbiota of domestic dogs has been adapted to domestication, which is of great significance for the ability of domestic dogs to adapt to changes in food composition.
30073560	5	60	theme	microbiota	1140:1149	arg1	metagenomes					1151:1161	the gut microbiota metagenomes	1132:1161	the gut microbiota metagenomes of domestic dogs and gray wolves	1132:1194	A significant difference in the abundance of genes encoding glycosyltransferase family 34 (GT34), carbohydrate-binding module family 25 (CBM25), and glycoside hydrolase family 13 (GH13) between the gut microbiota metagenomes of domestic dogs and gray wolves also supported this observation.
30073560	6	61	theme	dog	1255:1257	arg1	microbiota					1263:1272	the domestic dog gut microbiota	1242:1272	the domestic dog gut microbiota	1242:1272	Furthermore, the domestic dog gut microbiota has greater valine, leucine and isoleucine biosynthesis and nitrogen metabolism.
30073560	7	62	theme	smaller	1434:1440	arg1	amount					1442:1447	a smaller amount	1432:1447	a smaller amount	1432:1447	This result showed that compared with wolves, the domestic dog diet contains a smaller amount of animal protein, which is consistent with the dietary composition of wolves and dogs.
30073560	7	62	theme	smaller	1434:1440	arg1	protein					1459:1465	animal protein	1452:1465	animal protein	1452:1465	This result showed that compared with wolves, the domestic dog diet contains a smaller amount of animal protein, which is consistent with the dietary composition of wolves and dogs.
30073560	7	62	theme	smaller	1434:1440	arg1	consistent					1477:1486	consistent	1477:1486	consistent	1477:1486	This result showed that compared with wolves, the domestic dog diet contains a smaller amount of animal protein, which is consistent with the dietary composition of wolves and dogs.
30073560	0	63	theme	function	78:85	arg1	differentiation					39:53	the differentiation	35:53	the differentiation of the composition and function of gut microbiotas between domestic dogs (Canis lupus familiaris) and gray wolves (Canis lupus)	35:181	Changes in feeding habits promoted the differentiation of the composition and function of gut microbiotas between domestic dogs (Canis lupus familiaris) and gray wolves (Canis lupus).
30073560	4	64	from	numbers	762:768	arg1	dogs					782:785	domestic dogs	773:785	domestic dogs	773:785	Furthermore, a number of taxa observed in higher numbers in domestic dogs are related to carbohydrate metabolism, which may be because that there were more complicated polysaccharides in dogs diets than that in wolves diets.
30073560	0	65	theme	gut	90:92	arg1	microbiotas					94:104	gut microbiotas	90:104	gut microbiotas	90:104	Changes in feeding habits promoted the differentiation of the composition and function of gut microbiotas between domestic dogs (Canis lupus familiaris) and gray wolves (Canis lupus).
30073560	1	66	theme	great	285:289	arg1	differences					291:301	great differences	285:301	great differences in their diets and living environments	285:340	Wolves (Canis lupus) and their domesticated and close relatives, dogs (Canis lupus familiaris), have great differences in their diets and living environments.
30073560	7	67	theme	dog	1414:1416	arg1	diet					1418:1421	the domestic dog diet	1401:1421	the domestic dog diet	1401:1421	This result showed that compared with wolves, the domestic dog diet contains a smaller amount of animal protein, which is consistent with the dietary composition of wolves and dogs.
30073560	2	68	theme	gut	439:441	arg1	microbiota					443:452	the gut microbiota	435:452	the gut microbiota	435:452	To the best of our knowledge, the fundamental question of how the abundance and function of the gut microbiota of domestic dogs evolved to adapt to the changes in host feeding habits has yet to be addressed.
30073560	5	69	theme	gray	1184:1187	arg1	wolves					1189:1194	gray wolves	1184:1194	gray wolves	1184:1194	A significant difference in the abundance of genes encoding glycosyltransferase family 34 (GT34), carbohydrate-binding module family 25 (CBM25), and glycoside hydrolase family 13 (GH13) between the gut microbiota metagenomes of domestic dogs and gray wolves also supported this observation.
30073560	8	70	dep	function	1567:1574	arg1	the					1563:1565	the	1563:1565	the	1563:1565	Our results indicate that the function and abundance of gut microbiota of domestic dogs has been adapted to domestication, which is of great significance for the ability of domestic dogs to adapt to changes in food composition.
30073560	1	71	theme	Canis	192:196	arg1	lupus					198:202	Canis lupus	192:202	Canis lupus	192:202	Wolves (Canis lupus) and their domesticated and close relatives, dogs (Canis lupus familiaris), have great differences in their diets and living environments.
30073560	1	71	theme	Canis	192:196	arg1	Wolves					184:189	Wolves	184:189	Wolves (Canis lupus)	184:203	Wolves (Canis lupus) and their domesticated and close relatives, dogs (Canis lupus familiaris), have great differences in their diets and living environments.
30073560	1	72	from	differences	291:301	arg1	environments					329:340	living environments	322:340	living environments	322:340	Wolves (Canis lupus) and their domesticated and close relatives, dogs (Canis lupus familiaris), have great differences in their diets and living environments.
30073560	1	72	from	differences	291:301	arg1	diets					312:316	their diets	306:316	their diets	306:316	Wolves (Canis lupus) and their domesticated and close relatives, dogs (Canis lupus familiaris), have great differences in their diets and living environments.
30073560	0	73	dep	composition	62:72	arg1	the					58:60	the	58:60	the	58:60	Changes in feeding habits promoted the differentiation of the composition and function of gut microbiotas between domestic dogs (Canis lupus familiaris) and gray wolves (Canis lupus).
30073560	5	74	theme	dogs	1175:1178	arg1	metagenomes					1151:1161	the gut microbiota metagenomes	1132:1161	the gut microbiota metagenomes of domestic dogs and gray wolves	1132:1194	A significant difference in the abundance of genes encoding glycosyltransferase family 34 (GT34), carbohydrate-binding module family 25 (CBM25), and glycoside hydrolase family 13 (GH13) between the gut microbiota metagenomes of domestic dogs and gray wolves also supported this observation.
30073560	6	75	theme	greater	1278:1284	arg1	biosynthesis					1317:1328	greater valine, leucine and isoleucine biosynthesis	1278:1328	greater valine, leucine and isoleucine biosynthesis	1278:1328	Furthermore, the domestic dog gut microbiota has greater valine, leucine and isoleucine biosynthesis and nitrogen metabolism.
30073560	7	76	theme	domestic	1405:1412	arg1	diet					1418:1421	the domestic dog diet	1401:1421	the domestic dog diet	1401:1421	This result showed that compared with wolves, the domestic dog diet contains a smaller amount of animal protein, which is consistent with the dietary composition of wolves and dogs.
30073560	4	77	theme	complicated	869:879	arg1	polysaccharides					881:895	more complicated polysaccharides	864:895	more complicated polysaccharides	864:895	Furthermore, a number of taxa observed in higher numbers in domestic dogs are related to carbohydrate metabolism, which may be because that there were more complicated polysaccharides in dogs diets than that in wolves diets.
30073560	5	78	from	difference	952:961	arg1	abundance					970:978	the abundance	966:978	the abundance of genes encoding glycosyltransferase family 34 (GT34), carbohydrate-binding module family 25 (CBM25), and glycoside hydrolase family 13 (GH13)	966:1122	A significant difference in the abundance of genes encoding glycosyltransferase family 34 (GT34), carbohydrate-binding module family 25 (CBM25), and glycoside hydrolase family 13 (GH13) between the gut microbiota metagenomes of domestic dogs and gray wolves also supported this observation.
30073560	1	79	theme	Canis	255:259	arg1	dogs					249:252	dogs	249:252	dogs (Canis lupus familiaris)	249:277	Wolves (Canis lupus) and their domesticated and close relatives, dogs (Canis lupus familiaris), have great differences in their diets and living environments.
30073560	1	79	theme	Canis	255:259	arg1	familiaris					267:276	Canis lupus familiaris	255:276	Canis lupus familiaris	255:276	Wolves (Canis lupus) and their domesticated and close relatives, dogs (Canis lupus familiaris), have great differences in their diets and living environments.
30073560	2	80	dep	abundance	409:417	arg1	the					405:407	the	405:407	the	405:407	To the best of our knowledge, the fundamental question of how the abundance and function of the gut microbiota of domestic dogs evolved to adapt to the changes in host feeding habits has yet to be addressed.
30073560	2	81	theme	fundamental	377:387	arg1	question					389:396	the fundamental question	373:396	the fundamental question of how the abundance and function of the gut microbiota of domestic dogs evolved to adapt to the changes in host feeding habits	373:524	To the best of our knowledge, the fundamental question of how the abundance and function of the gut microbiota of domestic dogs evolved to adapt to the changes in host feeding habits has yet to be addressed.
30073560	1	82	contain	have	280:283	arg1	relatives					238:246	their domesticated and close relatives	209:246	their domesticated and close relatives	209:246	Wolves (Canis lupus) and their domesticated and close relatives, dogs (Canis lupus familiaris), have great differences in their diets and living environments.
30073560	1	82	contain	have	280:283	arg1	lupus					198:202	Canis lupus	192:202	Canis lupus	192:202	Wolves (Canis lupus) and their domesticated and close relatives, dogs (Canis lupus familiaris), have great differences in their diets and living environments.
30073560	1	82	contain	have	280:283	arg1	dogs					249:252	dogs	249:252	dogs (Canis lupus familiaris)	249:277	Wolves (Canis lupus) and their domesticated and close relatives, dogs (Canis lupus familiaris), have great differences in their diets and living environments.
30073560	1	82	contain	have	280:283	arg2	differences					291:301	great differences	285:301	great differences in their diets and living environments	285:340	Wolves (Canis lupus) and their domesticated and close relatives, dogs (Canis lupus familiaris), have great differences in their diets and living environments.
30073560	1	82	contain	have	280:283	arg1	Wolves					184:189	Wolves	184:189	Wolves (Canis lupus)	184:203	Wolves (Canis lupus) and their domesticated and close relatives, dogs (Canis lupus familiaris), have great differences in their diets and living environments.
30073560	5	83	theme	carbohydrate-binding	1036:1055	arg1	family					1064:1069	carbohydrate-binding module family 25 (CBM25)	1036:1080	carbohydrate-binding module family 25 (CBM25)	1036:1080	A significant difference in the abundance of genes encoding glycosyltransferase family 34 (GT34), carbohydrate-binding module family 25 (CBM25), and glycoside hydrolase family 13 (GH13) between the gut microbiota metagenomes of domestic dogs and gray wolves also supported this observation.
30073560	8	84	theme	great	1672:1676	arg1	significance					1678:1689	great significance	1672:1689	great significance	1672:1689	Our results indicate that the function and abundance of gut microbiota of domestic dogs has been adapted to domestication, which is of great significance for the ability of domestic dogs to adapt to changes in food composition.
30073560	3	85	contain	have	678:681	arg1	abundance					626:634	the abundance	622:634	the abundance of gut microbiota between the two species	622:676	In this study, our comparative analyses of gut metagenomes showed that the abundance of gut microbiota between the two species have some significant differences.
30073560	3	85	contain	have	678:681	arg2	differences					700:710	some significant differences	683:710	some significant differences	683:710	In this study, our comparative analyses of gut metagenomes showed that the abundance of gut microbiota between the two species have some significant differences.
30073560	5	86	theme	hydrolase	1097:1105	arg1	family					1107:1112	glycoside hydrolase family 13 (GH13)	1087:1122	glycoside hydrolase family 13 (GH13)	1087:1122	A significant difference in the abundance of genes encoding glycosyltransferase family 34 (GT34), carbohydrate-binding module family 25 (CBM25), and glycoside hydrolase family 13 (GH13) between the gut microbiota metagenomes of domestic dogs and gray wolves also supported this observation.
30073560	6	87	theme	nitrogen	1334:1341	arg1	metabolism					1343:1352	nitrogen metabolism	1334:1352	nitrogen metabolism	1334:1352	Furthermore, the domestic dog gut microbiota has greater valine, leucine and isoleucine biosynthesis and nitrogen metabolism.
29340658	13	0	theme	eggs	1752:1755	arg1	cuticle					1726:1732	the cuticle	1722:1732	the cuticle of brown unwashed eggs with hen age	1722:1768	A significant reduction in the amount of proteins and phosphates and polysaccharides was observed in the cuticle of brown unwashed eggs with hen age.
29340658	8	1	theme	cuticle	1101:1107	arg1	length					1114:1119	cuticle pore length	1101:1119	cuticle pore length	1101:1119	The eggshell thickness and cuticle pore length visualized in brown eggs was significantly higher than in white eggs in hens of all ages.
29340658	4	2	theme	eggshell	564:571	arg1	cuticle					573:579	the eggshell cuticle	560:579	the eggshell cuticle	560:579	The present study characterizes the chemical composition of the eggshell cuticle and structure of pore plugs from table eggs.
29340658	7	3	theme	commercial	1046:1055	arg1	process					1065:1071	the commercial washing process	1042:1071	the commercial washing process	1042:1071	SEM observations confirmed that the plug formed by the cuticle layer within the eggshell pore remains firmly lodged throughout the commercial washing process.
29340658	8	4	theme	white	1179:1183	arg1	eggs					1185:1188	white eggs	1179:1188	white eggs	1179:1188	The eggshell thickness and cuticle pore length visualized in brown eggs was significantly higher than in white eggs in hens of all ages.
29340658	10	5	theme	Detailed	1357:1364	arg1	assessment					1366:1375	Detailed assessment	1357:1375	Detailed assessment of the cuticle chemical composition	1357:1411	Detailed assessment of the cuticle chemical composition was performed by Fourier transform infrared spectroscopy (FTIR).
29340658	14	6	theme	hen	1828:1830	arg1	age					1832:1834	hen age	1828:1834	hen age	1828:1834	In white unwashed eggs, amides and lipids decreased with hen age; by contrast, the amount of sulfate was highest at mid-lay.
29340658	4	7	theme	plugs	603:607	arg1	composition					545:555	the chemical composition	532:555	the chemical composition of the eggshell cuticle	532:579	The present study characterizes the chemical composition of the eggshell cuticle and structure of pore plugs from table eggs.
29340658	4	7	theme	plugs	603:607	arg1	structure					585:593	structure	585:593	structure of pore plugs from table eggs	585:623	The present study characterizes the chemical composition of the eggshell cuticle and structure of pore plugs from table eggs.
29340658	5	8	theme	egg	667:669	arg1	laying					671:676	both brown and white egg laying	646:676	both brown and white egg laying Lohmann flocks (early, mid, and late lay)	646:718	Eggs collected from both brown and white egg laying Lohmann flocks (early, mid, and late lay) were either unwashed or washed.
29340658	2	9	theme	defense	249:255	arg1	line					241:244	the first line	231:244	the first line of defense to restrict the entry of egg-associated pathogens, such as Salmonella Enteritidis	231:337	The eggshell cuticle is the first line of defense to restrict the entry of egg-associated pathogens, such as Salmonella Enteritidis.
29340658	2	9	theme	defense	249:255	arg1	cuticle					220:226	The eggshell cuticle	207:226	The eggshell cuticle	207:226	The eggshell cuticle is the first line of defense to restrict the entry of egg-associated pathogens, such as Salmonella Enteritidis.
29340658	4	10	theme	table	614:618	arg1	eggs					620:623	table eggs	614:623	table eggs	614:623	The present study characterizes the chemical composition of the eggshell cuticle and structure of pore plugs from table eggs.
29340658	5	11	theme	Lohmann	678:684	arg1	flocks					686:691	both brown and white egg laying Lohmann flocks	646:691	both brown and white egg laying Lohmann flocks (early, mid, and late lay)	646:718	Eggs collected from both brown and white egg laying Lohmann flocks (early, mid, and late lay) were either unwashed or washed.
29340658	1	12	theme	foodborne	174:182	arg1	illness					184:190	foodborne illness	174:190	foodborne illness	174:190	Food safety of table eggs is vital since many pathogens can contaminate the unfertilized egg, leading to increased risk of foodborne illness for consumers.
29340658	6	13	theme	energy-dispersive	871:887	arg1	EDS					909:911	EDS	909:911	EDS	909:911	Pore plugs were characterized using scanning electron microscopy (SEM), and elemental composition was determined using energy-dispersive x-ray spectroscopy (EDS).
29340658	6	13	theme	energy-dispersive	871:887	arg1	spectroscopy					895:906	energy-dispersive x-ray spectroscopy	871:906	energy-dispersive x-ray spectroscopy (EDS)	871:912	Pore plugs were characterized using scanning electron microscopy (SEM), and elemental composition was determined using energy-dispersive x-ray spectroscopy (EDS).
29340658	5	14	dep	early	694:698	arg1	lay					715:717	lay	715:717	lay	715:717	Eggs collected from both brown and white egg laying Lohmann flocks (early, mid, and late lay) were either unwashed or washed.
29340658	14	15	theme	unwashed	1780:1787	arg1	eggs					1789:1792	white unwashed eggs	1774:1792	white unwashed eggs	1774:1792	In white unwashed eggs, amides and lipids decreased with hen age; by contrast, the amount of sulfate was highest at mid-lay.
29340658	5	16	dep	flocks	686:691	arg1	early					694:698	early	694:698	early	694:698	Eggs collected from both brown and white egg laying Lohmann flocks (early, mid, and late lay) were either unwashed or washed.
29340658	5	16	dep	flocks	686:691	arg1	mid					701:703	mid	701:703	mid	701:703	Eggs collected from both brown and white egg laying Lohmann flocks (early, mid, and late lay) were either unwashed or washed.
29340658	5	16	dep	flocks	686:691	arg1	late					710:713	late	710:713	late	710:713	Eggs collected from both brown and white egg laying Lohmann flocks (early, mid, and late lay) were either unwashed or washed.
29340658	14	17	theme	white	1774:1778	arg1	eggs					1789:1792	white unwashed eggs	1774:1792	white unwashed eggs	1774:1792	In white unwashed eggs, amides and lipids decreased with hen age; by contrast, the amount of sulfate was highest at mid-lay.
29340658	4	18	from	structure	585:593	arg1	eggs					620:623	table eggs	614:623	table eggs	614:623	The present study characterizes the chemical composition of the eggshell cuticle and structure of pore plugs from table eggs.
29340658	10	19	theme	cuticle	1384:1390	arg1	composition					1401:1411	the cuticle chemical composition	1380:1411	the cuticle chemical composition	1380:1411	Detailed assessment of the cuticle chemical composition was performed by Fourier transform infrared spectroscopy (FTIR).
29340658	1	20	theme	unfertilized	127:138	arg1	egg					140:142	the unfertilized egg	123:142	the unfertilized egg	123:142	Food safety of table eggs is vital since many pathogens can contaminate the unfertilized egg, leading to increased risk of foodborne illness for consumers.
29340658	12	21	theme	hen	1597:1599	arg1	age					1601:1603	hen age	1597:1603	hen age for white eggs	1597:1618	There was a trend of lower cuticle coverage with increasing hen age for white eggs.
29340658	8	22	dep	thickness	1087:1095	arg1	The					1074:1076	The	1074:1076	The	1074:1076	The eggshell thickness and cuticle pore length visualized in brown eggs was significantly higher than in white eggs in hens of all ages.
29340658	3	23	theme	cuticle	390:396	arg1	layer					398:402	the cuticle layer	386:402	the cuticle layer	386:402	The thickness and completeness of coverage of the cuticle layer are heritable traits that are strongly associated with egg resistance to bacterial penetration.
29340658	10	24	dep	transform	1438:1446	arg1	infrared					1448:1455	infrared	1448:1455	transform infrared spectroscopy (FTIR)	1438:1475	Detailed assessment of the cuticle chemical composition was performed by Fourier transform infrared spectroscopy (FTIR).
29340658	9	25	theme	pore	1242:1245	arg1	enriched					1265:1272	enriched	1265:1272	enriched	1265:1272	EDS analysis revealed that the pore inner surface was enriched in phosphorus and chemically different from the surrounding bulk eggshell mineral.
29340658	9	25	theme	pore	1242:1245	arg1	mineral					1348:1354	mineral	1348:1354	mineral	1348:1354	EDS analysis revealed that the pore inner surface was enriched in phosphorus and chemically different from the surrounding bulk eggshell mineral.
29340658	9	25	theme	pore	1242:1245	arg1	surface					1253:1259	the pore inner surface	1238:1259	the pore inner surface	1238:1259	EDS analysis revealed that the pore inner surface was enriched in phosphorus and chemically different from the surrounding bulk eggshell mineral.
29340658	9	25	theme	pore	1242:1245	arg1	different					1303:1311	different	1303:1311	different	1303:1311	EDS analysis revealed that the pore inner surface was enriched in phosphorus and chemically different from the surrounding bulk eggshell mineral.
29340658	15	26	theme	breeding	1948:1955	arg1	programs					1957:1964	selective breeding programs	1938:1964	selective breeding programs that target cuticle integrity and pore plug stability to enhance egg resistance to pathogen penetration and improve food safety	1938:2092	The results from our research will assist selective breeding programs that target cuticle integrity and pore plug stability to enhance egg resistance to pathogen penetration and improve food safety.
29340658	0	27	from	properties	22:31	arg1	egg					46:48	the table egg	36:48	the table egg	36:48	Cuticle and pore plug properties in the table egg.
29340658	10	28	theme	composition	1401:1411	arg1	assessment					1366:1375	Detailed assessment	1357:1375	Detailed assessment of the cuticle chemical composition	1357:1411	Detailed assessment of the cuticle chemical composition was performed by Fourier transform infrared spectroscopy (FTIR).
29340658	1	29	theme	table	66:70	arg1	eggs					72:75	table eggs	66:75	table eggs	66:75	Food safety of table eggs is vital since many pathogens can contaminate the unfertilized egg, leading to increased risk of foodborne illness for consumers.
29340658	12	30	theme	cuticle	1564:1570	arg1	coverage					1572:1579	lower cuticle coverage	1558:1579	lower cuticle coverage	1558:1579	There was a trend of lower cuticle coverage with increasing hen age for white eggs.
29340658	13	31	theme	hen	1762:1764	arg1	age					1766:1768	hen age	1762:1768	hen age	1762:1768	A significant reduction in the amount of proteins and phosphates and polysaccharides was observed in the cuticle of brown unwashed eggs with hen age.
29340658	6	32	theme	scanning	788:795	arg1	SEM					818:820	SEM	818:820	SEM	818:820	Pore plugs were characterized using scanning electron microscopy (SEM), and elemental composition was determined using energy-dispersive x-ray spectroscopy (EDS).
29340658	6	32	theme	scanning	788:795	arg1	microscopy					806:815	scanning electron microscopy	788:815	scanning electron microscopy (SEM)	788:821	Pore plugs were characterized using scanning electron microscopy (SEM), and elemental composition was determined using energy-dispersive x-ray spectroscopy (EDS).
29340658	2	33	theme	pathogens	297:305	arg1	entry					273:277	the entry	269:277	the entry of egg-associated pathogens, such as Salmonella Enteritidis	269:337	The eggshell cuticle is the first line of defense to restrict the entry of egg-associated pathogens, such as Salmonella Enteritidis.
29340658	15	34	theme	plug	2005:2008	arg1	stability					2010:2018	pore plug stability	2000:2018	pore plug stability	2000:2018	The results from our research will assist selective breeding programs that target cuticle integrity and pore plug stability to enhance egg resistance to pathogen penetration and improve food safety.
29340658	1	35	theme	illness	184:190	arg1	risk					166:169	increased risk	156:169	increased risk of foodborne illness for consumers	156:204	Food safety of table eggs is vital since many pathogens can contaminate the unfertilized egg, leading to increased risk of foodborne illness for consumers.
29340658	4	36	theme	cuticle	573:579	arg1	composition					545:555	the chemical composition	532:555	the chemical composition of the eggshell cuticle	532:579	The present study characterizes the chemical composition of the eggshell cuticle and structure of pore plugs from table eggs.
29340658	4	36	theme	cuticle	573:579	arg1	structure					585:593	structure	585:593	structure of pore plugs from table eggs	585:623	The present study characterizes the chemical composition of the eggshell cuticle and structure of pore plugs from table eggs.
29340658	3	37	theme	heritable	408:416	arg1	completeness					358:369	completeness	358:369	completeness	358:369	The thickness and completeness of coverage of the cuticle layer are heritable traits that are strongly associated with egg resistance to bacterial penetration.
29340658	3	37	theme	heritable	408:416	arg1	thickness					344:352	thickness	344:352	thickness	344:352	The thickness and completeness of coverage of the cuticle layer are heritable traits that are strongly associated with egg resistance to bacterial penetration.
29340658	3	37	theme	heritable	408:416	arg1	traits					418:423	heritable traits	408:423	heritable traits that are strongly associated with egg resistance to bacterial penetration	408:497	The thickness and completeness of coverage of the cuticle layer are heritable traits that are strongly associated with egg resistance to bacterial penetration.
29340658	9	38	theme	surrounding	1322:1332	arg1	eggshell					1339:1346	the surrounding bulk eggshell	1318:1346	the surrounding bulk eggshell	1318:1346	EDS analysis revealed that the pore inner surface was enriched in phosphorus and chemically different from the surrounding bulk eggshell mineral.
29340658	15	39	theme	cuticle	1978:1984	arg1	integrity					1986:1994	cuticle integrity	1978:1994	cuticle integrity	1978:1994	The results from our research will assist selective breeding programs that target cuticle integrity and pore plug stability to enhance egg resistance to pathogen penetration and improve food safety.
29340658	13	40	theme	unwashed	1743:1750	arg1	eggs					1752:1755	brown unwashed eggs	1737:1755	brown unwashed eggs	1737:1755	A significant reduction in the amount of proteins and phosphates and polysaccharides was observed in the cuticle of brown unwashed eggs with hen age.
29340658	7	41	theme	washing	1057:1063	arg1	process					1065:1071	the commercial washing process	1042:1071	the commercial washing process	1042:1071	SEM observations confirmed that the plug formed by the cuticle layer within the eggshell pore remains firmly lodged throughout the commercial washing process.
29340658	3	42	theme	egg	459:461	arg1	resistance					463:472	egg resistance	459:472	egg resistance to bacterial penetration	459:497	The thickness and completeness of coverage of the cuticle layer are heritable traits that are strongly associated with egg resistance to bacterial penetration.
29340658	8	43	theme	eggshell	1078:1085	arg1	thickness					1087:1095	eggshell thickness	1078:1095	eggshell thickness	1078:1095	The eggshell thickness and cuticle pore length visualized in brown eggs was significantly higher than in white eggs in hens of all ages.
29340658	6	44	theme	elemental	828:836	arg1	composition					838:848	elemental composition	828:848	elemental composition	828:848	Pore plugs were characterized using scanning electron microscopy (SEM), and elemental composition was determined using energy-dispersive x-ray spectroscopy (EDS).
29340658	13	45	theme	significant	1623:1633	arg1	reduction					1635:1643	A significant reduction	1621:1643	A significant reduction in the amount of proteins and phosphates and polysaccharides	1621:1704	A significant reduction in the amount of proteins and phosphates and polysaccharides was observed in the cuticle of brown unwashed eggs with hen age.
29340658	2	46	theme	first	235:239	arg1	line					241:244	the first line	231:244	the first line of defense to restrict the entry of egg-associated pathogens, such as Salmonella Enteritidis	231:337	The eggshell cuticle is the first line of defense to restrict the entry of egg-associated pathogens, such as Salmonella Enteritidis.
29340658	2	46	theme	first	235:239	arg1	cuticle					220:226	The eggshell cuticle	207:226	The eggshell cuticle	207:226	The eggshell cuticle is the first line of defense to restrict the entry of egg-associated pathogens, such as Salmonella Enteritidis.
29340658	8	47	theme	pore	1109:1112	arg1	length					1114:1119	cuticle pore length	1101:1119	cuticle pore length	1101:1119	The eggshell thickness and cuticle pore length visualized in brown eggs was significantly higher than in white eggs in hens of all ages.
29340658	13	48	theme	phosphates	1675:1684	arg1	proteins					1662:1669	proteins	1662:1669	proteins	1662:1669	A significant reduction in the amount of proteins and phosphates and polysaccharides was observed in the cuticle of brown unwashed eggs with hen age.
29340658	13	48	theme	phosphates	1675:1684	arg1	amount					1652:1657	the amount	1648:1657	the amount of proteins and phosphates and polysaccharides	1648:1704	A significant reduction in the amount of proteins and phosphates and polysaccharides was observed in the cuticle of brown unwashed eggs with hen age.
29340658	13	48	theme	phosphates	1675:1684	arg1	phosphates					1675:1684	phosphates	1675:1684	phosphates	1675:1684	A significant reduction in the amount of proteins and phosphates and polysaccharides was observed in the cuticle of brown unwashed eggs with hen age.
29340658	4	49	from	eggs	620:623	arg1	composition					545:555	the chemical composition	532:555	the chemical composition of the eggshell cuticle	532:579	The present study characterizes the chemical composition of the eggshell cuticle and structure of pore plugs from table eggs.
29340658	4	49	from	eggs	620:623	arg1	structure					585:593	structure	585:593	structure of pore plugs from table eggs	585:623	The present study characterizes the chemical composition of the eggshell cuticle and structure of pore plugs from table eggs.
29340658	4	49	from	eggs	620:623	arg1	plugs					603:607	pore plugs	598:607	pore plugs from table eggs	598:623	The present study characterizes the chemical composition of the eggshell cuticle and structure of pore plugs from table eggs.
29340658	15	50	theme	pathogen	2049:2056	arg1	penetration					2058:2068	pathogen penetration	2049:2068	pathogen penetration	2049:2068	The results from our research will assist selective breeding programs that target cuticle integrity and pore plug stability to enhance egg resistance to pathogen penetration and improve food safety.
29340658	13	51	located	observed	1710:1717	arg2	reduction					1635:1643	A significant reduction	1621:1643	A significant reduction in the amount of proteins and phosphates and polysaccharides	1621:1704	A significant reduction in the amount of proteins and phosphates and polysaccharides was observed in the cuticle of brown unwashed eggs with hen age.
29340658	13	51	located	observed	1710:1717	arg1	cuticle					1726:1732	the cuticle	1722:1732	the cuticle of brown unwashed eggs with hen age	1722:1768	A significant reduction in the amount of proteins and phosphates and polysaccharides was observed in the cuticle of brown unwashed eggs with hen age.
29340658	5	52	theme	white	661:665	arg1	laying					671:676	both brown and white egg laying	646:676	both brown and white egg laying Lohmann flocks (early, mid, and late lay)	646:718	Eggs collected from both brown and white egg laying Lohmann flocks (early, mid, and late lay) were either unwashed or washed.
29340658	1	53	theme	increased	156:164	arg1	risk					166:169	increased risk	156:169	increased risk of foodborne illness for consumers	156:204	Food safety of table eggs is vital since many pathogens can contaminate the unfertilized egg, leading to increased risk of foodborne illness for consumers.
29340658	8	54	theme	brown	1135:1139	arg1	eggs					1141:1144	brown eggs	1135:1144	brown eggs	1135:1144	The eggshell thickness and cuticle pore length visualized in brown eggs was significantly higher than in white eggs in hens of all ages.
29340658	4	55	theme	pore	598:601	arg1	plugs					603:607	pore plugs	598:607	pore plugs from table eggs	598:623	The present study characterizes the chemical composition of the eggshell cuticle and structure of pore plugs from table eggs.
29340658	6	56	theme	x-ray	889:893	arg1	EDS					909:911	EDS	909:911	EDS	909:911	Pore plugs were characterized using scanning electron microscopy (SEM), and elemental composition was determined using energy-dispersive x-ray spectroscopy (EDS).
29340658	6	56	theme	x-ray	889:893	arg1	spectroscopy					895:906	energy-dispersive x-ray spectroscopy	871:906	energy-dispersive x-ray spectroscopy (EDS)	871:912	Pore plugs were characterized using scanning electron microscopy (SEM), and elemental composition was determined using energy-dispersive x-ray spectroscopy (EDS).
29340658	8	57	theme	ages	1205:1208	arg1	hens					1193:1196	hens	1193:1196	hens of all ages	1193:1208	The eggshell thickness and cuticle pore length visualized in brown eggs was significantly higher than in white eggs in hens of all ages.
29340658	15	58	theme	egg	2031:2033	arg1	resistance					2035:2044	egg resistance	2031:2044	egg resistance to pathogen penetration	2031:2068	The results from our research will assist selective breeding programs that target cuticle integrity and pore plug stability to enhance egg resistance to pathogen penetration and improve food safety.
29340658	13	59	theme	proteins	1662:1669	arg1	proteins					1662:1669	proteins	1662:1669	proteins	1662:1669	A significant reduction in the amount of proteins and phosphates and polysaccharides was observed in the cuticle of brown unwashed eggs with hen age.
29340658	13	59	theme	proteins	1662:1669	arg1	amount					1652:1657	the amount	1648:1657	the amount of proteins and phosphates and polysaccharides	1648:1704	A significant reduction in the amount of proteins and phosphates and polysaccharides was observed in the cuticle of brown unwashed eggs with hen age.
29340658	13	59	theme	proteins	1662:1669	arg1	phosphates					1675:1684	phosphates	1675:1684	phosphates	1675:1684	A significant reduction in the amount of proteins and phosphates and polysaccharides was observed in the cuticle of brown unwashed eggs with hen age.
29340658	7	60	theme	cuticle	970:976	arg1	layer					978:982	the cuticle layer	966:982	the cuticle layer within the eggshell pore	966:1007	SEM observations confirmed that the plug formed by the cuticle layer within the eggshell pore remains firmly lodged throughout the commercial washing process.
29340658	7	61	theme	eggshell	995:1002	arg1	pore					1004:1007	the eggshell pore	991:1007	the eggshell pore	991:1007	SEM observations confirmed that the plug formed by the cuticle layer within the eggshell pore remains firmly lodged throughout the commercial washing process.
29340658	5	62	theme	brown	651:655	arg1	laying					671:676	both brown and white egg laying	646:676	both brown and white egg laying Lohmann flocks (early, mid, and late lay)	646:718	Eggs collected from both brown and white egg laying Lohmann flocks (early, mid, and late lay) were either unwashed or washed.
29340658	1	63	theme	many	92:95	arg1	pathogens					97:105	many pathogens	92:105	many pathogens	92:105	Food safety of table eggs is vital since many pathogens can contaminate the unfertilized egg, leading to increased risk of foodborne illness for consumers.
29340658	2	64	theme	eggshell	211:218	arg1	line					241:244	the first line	231:244	the first line of defense to restrict the entry of egg-associated pathogens, such as Salmonella Enteritidis	231:337	The eggshell cuticle is the first line of defense to restrict the entry of egg-associated pathogens, such as Salmonella Enteritidis.
29340658	2	64	theme	eggshell	211:218	arg1	cuticle					220:226	The eggshell cuticle	207:226	The eggshell cuticle	207:226	The eggshell cuticle is the first line of defense to restrict the entry of egg-associated pathogens, such as Salmonella Enteritidis.
29340658	9	65	dep	enriched	1265:1272	arg1	enriched					1265:1272	enriched	1265:1272	enriched	1265:1272	EDS analysis revealed that the pore inner surface was enriched in phosphorus and chemically different from the surrounding bulk eggshell mineral.
29340658	9	65	dep	enriched	1265:1272	arg1	mineral					1348:1354	mineral	1348:1354	mineral	1348:1354	EDS analysis revealed that the pore inner surface was enriched in phosphorus and chemically different from the surrounding bulk eggshell mineral.
29340658	9	65	dep	enriched	1265:1272	arg1	surface					1253:1259	the pore inner surface	1238:1259	the pore inner surface	1238:1259	EDS analysis revealed that the pore inner surface was enriched in phosphorus and chemically different from the surrounding bulk eggshell mineral.
29340658	9	65	dep	enriched	1265:1272	arg1	different					1303:1311	different	1303:1311	different	1303:1311	EDS analysis revealed that the pore inner surface was enriched in phosphorus and chemically different from the surrounding bulk eggshell mineral.
29340658	9	66	theme	EDS	1211:1213	arg1	analysis					1215:1222	EDS analysis	1211:1222	EDS analysis	1211:1222	EDS analysis revealed that the pore inner surface was enriched in phosphorus and chemically different from the surrounding bulk eggshell mineral.
29340658	7	67	theme	SEM	915:917	arg1	observations					919:930	SEM observations	915:930	SEM observations	915:930	SEM observations confirmed that the plug formed by the cuticle layer within the eggshell pore remains firmly lodged throughout the commercial washing process.
29340658	15	68	theme	food	2082:2085	arg1	safety					2087:2092	food safety	2082:2092	food safety	2082:2092	The results from our research will assist selective breeding programs that target cuticle integrity and pore plug stability to enhance egg resistance to pathogen penetration and improve food safety.
29340658	12	69	theme	white	1609:1613	arg1	eggs					1615:1618	white eggs	1609:1618	white eggs	1609:1618	There was a trend of lower cuticle coverage with increasing hen age for white eggs.
29340658	10	70	dep	Fourier	1430:1436	arg1	transform					1438:1446	transform	1438:1446	transform infrared spectroscopy (FTIR)	1438:1475	Detailed assessment of the cuticle chemical composition was performed by Fourier transform infrared spectroscopy (FTIR).
29340658	9	71	from	phosphorus	1277:1286	arg1	enriched					1265:1272	enriched	1265:1272	enriched	1265:1272	EDS analysis revealed that the pore inner surface was enriched in phosphorus and chemically different from the surrounding bulk eggshell mineral.
29340658	9	71	from	phosphorus	1277:1286	arg1	mineral					1348:1354	mineral	1348:1354	mineral	1348:1354	EDS analysis revealed that the pore inner surface was enriched in phosphorus and chemically different from the surrounding bulk eggshell mineral.
29340658	9	71	from	phosphorus	1277:1286	arg1	surface					1253:1259	the pore inner surface	1238:1259	the pore inner surface	1238:1259	EDS analysis revealed that the pore inner surface was enriched in phosphorus and chemically different from the surrounding bulk eggshell mineral.
29340658	9	71	from	phosphorus	1277:1286	arg1	different					1303:1311	different	1303:1311	different	1303:1311	EDS analysis revealed that the pore inner surface was enriched in phosphorus and chemically different from the surrounding bulk eggshell mineral.
29340658	9	72	from	enriched	1265:1272	arg1	phosphorus					1277:1286	phosphorus	1277:1286	phosphorus	1277:1286	EDS analysis revealed that the pore inner surface was enriched in phosphorus and chemically different from the surrounding bulk eggshell mineral.
29340658	1	73	theme	Food	51:54	arg1	safety					56:61	Food safety	51:61	Food safety of table eggs	51:75	Food safety of table eggs is vital since many pathogens can contaminate the unfertilized egg, leading to increased risk of foodborne illness for consumers.
29340658	3	74	theme	layer	398:402	arg1	coverage					374:381	coverage	374:381	coverage of the cuticle layer	374:402	The thickness and completeness of coverage of the cuticle layer are heritable traits that are strongly associated with egg resistance to bacterial penetration.
29340658	9	75	theme	inner	1247:1251	arg1	enriched					1265:1272	enriched	1265:1272	enriched	1265:1272	EDS analysis revealed that the pore inner surface was enriched in phosphorus and chemically different from the surrounding bulk eggshell mineral.
29340658	9	75	theme	inner	1247:1251	arg1	mineral					1348:1354	mineral	1348:1354	mineral	1348:1354	EDS analysis revealed that the pore inner surface was enriched in phosphorus and chemically different from the surrounding bulk eggshell mineral.
29340658	9	75	theme	inner	1247:1251	arg1	surface					1253:1259	the pore inner surface	1238:1259	the pore inner surface	1238:1259	EDS analysis revealed that the pore inner surface was enriched in phosphorus and chemically different from the surrounding bulk eggshell mineral.
29340658	9	75	theme	inner	1247:1251	arg1	different					1303:1311	different	1303:1311	different	1303:1311	EDS analysis revealed that the pore inner surface was enriched in phosphorus and chemically different from the surrounding bulk eggshell mineral.
29340658	10	76	theme	chemical	1392:1399	arg1	composition					1401:1411	the cuticle chemical composition	1380:1411	the cuticle chemical composition	1380:1411	Detailed assessment of the cuticle chemical composition was performed by Fourier transform infrared spectroscopy (FTIR).
29340658	1	77	theme	eggs	72:75	arg1	safety					56:61	Food safety	51:61	Food safety of table eggs	51:75	Food safety of table eggs is vital since many pathogens can contaminate the unfertilized egg, leading to increased risk of foodborne illness for consumers.
29340658	12	78	theme	lower	1558:1562	arg1	coverage					1572:1579	lower cuticle coverage	1558:1579	lower cuticle coverage	1558:1579	There was a trend of lower cuticle coverage with increasing hen age for white eggs.
29340658	4	79	theme	chemical	536:543	arg1	composition					545:555	the chemical composition	532:555	the chemical composition of the eggshell cuticle	532:579	The present study characterizes the chemical composition of the eggshell cuticle and structure of pore plugs from table eggs.
29340658	15	80	theme	selective	1938:1946	arg1	programs					1957:1964	selective breeding programs	1938:1964	selective breeding programs that target cuticle integrity and pore plug stability to enhance egg resistance to pathogen penetration and improve food safety	1938:2092	The results from our research will assist selective breeding programs that target cuticle integrity and pore plug stability to enhance egg resistance to pathogen penetration and improve food safety.
29340658	9	81	from	eggshell	1339:1346	arg1	enriched					1265:1272	enriched	1265:1272	enriched	1265:1272	EDS analysis revealed that the pore inner surface was enriched in phosphorus and chemically different from the surrounding bulk eggshell mineral.
29340658	9	81	from	eggshell	1339:1346	arg1	mineral					1348:1354	mineral	1348:1354	mineral	1348:1354	EDS analysis revealed that the pore inner surface was enriched in phosphorus and chemically different from the surrounding bulk eggshell mineral.
29340658	9	81	from	eggshell	1339:1346	arg1	surface					1253:1259	the pore inner surface	1238:1259	the pore inner surface	1238:1259	EDS analysis revealed that the pore inner surface was enriched in phosphorus and chemically different from the surrounding bulk eggshell mineral.
29340658	9	81	from	eggshell	1339:1346	arg1	different					1303:1311	different	1303:1311	different	1303:1311	EDS analysis revealed that the pore inner surface was enriched in phosphorus and chemically different from the surrounding bulk eggshell mineral.
29340658	6	82	theme	electron	797:804	arg1	SEM					818:820	SEM	818:820	SEM	818:820	Pore plugs were characterized using scanning electron microscopy (SEM), and elemental composition was determined using energy-dispersive x-ray spectroscopy (EDS).
29340658	6	82	theme	electron	797:804	arg1	microscopy					806:815	scanning electron microscopy	788:815	scanning electron microscopy (SEM)	788:821	Pore plugs were characterized using scanning electron microscopy (SEM), and elemental composition was determined using energy-dispersive x-ray spectroscopy (EDS).
29340658	14	83	theme	sulfate	1864:1870	arg1	amount					1854:1859	the amount	1850:1859	the amount of sulfate	1850:1870	In white unwashed eggs, amides and lipids decreased with hen age; by contrast, the amount of sulfate was highest at mid-lay.
29340658	14	83	theme	sulfate	1864:1870	arg1	highest					1876:1882	highest	1876:1882	highest	1876:1882	In white unwashed eggs, amides and lipids decreased with hen age; by contrast, the amount of sulfate was highest at mid-lay.
29340658	14	83	theme	sulfate	1864:1870	arg1	sulfate					1864:1870	sulfate	1864:1870	sulfate	1864:1870	In white unwashed eggs, amides and lipids decreased with hen age; by contrast, the amount of sulfate was highest at mid-lay.
29340658	12	84	theme	coverage	1572:1579	arg1	trend					1549:1553	a trend	1547:1553	a trend of lower cuticle coverage	1547:1579	There was a trend of lower cuticle coverage with increasing hen age for white eggs.
29340658	3	85	theme	coverage	374:381	arg1	completeness					358:369	completeness	358:369	completeness	358:369	The thickness and completeness of coverage of the cuticle layer are heritable traits that are strongly associated with egg resistance to bacterial penetration.
29340658	3	85	theme	coverage	374:381	arg1	thickness					344:352	thickness	344:352	thickness	344:352	The thickness and completeness of coverage of the cuticle layer are heritable traits that are strongly associated with egg resistance to bacterial penetration.
29340658	3	85	theme	coverage	374:381	arg1	traits					418:423	heritable traits	408:423	heritable traits that are strongly associated with egg resistance to bacterial penetration	408:497	The thickness and completeness of coverage of the cuticle layer are heritable traits that are strongly associated with egg resistance to bacterial penetration.
29340658	13	86	with	cuticle	1726:1732	arg1	age					1766:1768	hen age	1762:1768	hen age	1762:1768	A significant reduction in the amount of proteins and phosphates and polysaccharides was observed in the cuticle of brown unwashed eggs with hen age.
29340658	4	87	from	composition	545:555	arg1	eggs					620:623	table eggs	614:623	table eggs	614:623	The present study characterizes the chemical composition of the eggshell cuticle and structure of pore plugs from table eggs.
29340658	0	88	theme	table	40:44	arg1	egg					46:48	the table egg	36:48	the table egg	36:48	Cuticle and pore plug properties in the table egg.
29340658	15	89	theme	pore	2000:2003	arg1	stability					2010:2018	pore plug stability	2000:2018	pore plug stability	2000:2018	The results from our research will assist selective breeding programs that target cuticle integrity and pore plug stability to enhance egg resistance to pathogen penetration and improve food safety.
29340658	4	90	theme	present	504:510	arg1	study					512:516	The present study	500:516	The present study	500:516	The present study characterizes the chemical composition of the eggshell cuticle and structure of pore plugs from table eggs.
29340658	11	91	theme	eggshell	1519:1526	arg1	surface					1528:1534	the eggshell surface	1515:1534	the eggshell surface	1515:1534	Washing of eggs removed cuticle from the eggshell surface.
29340658	2	92	theme	egg-associated	282:295	arg1	Enteritidis					327:337	Salmonella Enteritidis	316:337	Salmonella Enteritidis	316:337	The eggshell cuticle is the first line of defense to restrict the entry of egg-associated pathogens, such as Salmonella Enteritidis.
29340658	2	92	theme	egg-associated	282:295	arg1	pathogens					297:305	egg-associated pathogens	282:305	egg-associated pathogens	282:305	The eggshell cuticle is the first line of defense to restrict the entry of egg-associated pathogens, such as Salmonella Enteritidis.
29340658	13	93	from	reduction	1635:1643	arg1	proteins					1662:1669	proteins	1662:1669	proteins	1662:1669	A significant reduction in the amount of proteins and phosphates and polysaccharides was observed in the cuticle of brown unwashed eggs with hen age.
29340658	13	93	from	reduction	1635:1643	arg1	amount					1652:1657	the amount	1648:1657	the amount of proteins and phosphates and polysaccharides	1648:1704	A significant reduction in the amount of proteins and phosphates and polysaccharides was observed in the cuticle of brown unwashed eggs with hen age.
29340658	13	93	from	reduction	1635:1643	arg1	phosphates					1675:1684	phosphates	1675:1684	phosphates	1675:1684	A significant reduction in the amount of proteins and phosphates and polysaccharides was observed in the cuticle of brown unwashed eggs with hen age.
29340658	15	94	from	research	1917:1924	arg1	results					1900:1906	The results	1896:1906	The results from our research	1896:1924	The results from our research will assist selective breeding programs that target cuticle integrity and pore plug stability to enhance egg resistance to pathogen penetration and improve food safety.
29340658	6	95	theme	Pore	752:755	arg1	plugs					757:761	Pore plugs	752:761	Pore plugs	752:761	Pore plugs were characterized using scanning electron microscopy (SEM), and elemental composition was determined using energy-dispersive x-ray spectroscopy (EDS).
29340658	13	96	theme	brown	1737:1741	arg1	eggs					1752:1755	brown unwashed eggs	1737:1755	brown unwashed eggs	1737:1755	A significant reduction in the amount of proteins and phosphates and polysaccharides was observed in the cuticle of brown unwashed eggs with hen age.
29340658	3	97	theme	bacterial	477:485	arg1	penetration					487:497	bacterial penetration	477:497	bacterial penetration	477:497	The thickness and completeness of coverage of the cuticle layer are heritable traits that are strongly associated with egg resistance to bacterial penetration.
29340658	9	98	theme	bulk	1334:1337	arg1	eggshell					1339:1346	the surrounding bulk eggshell	1318:1346	the surrounding bulk eggshell	1318:1346	EDS analysis revealed that the pore inner surface was enriched in phosphorus and chemically different from the surrounding bulk eggshell mineral.
30918062	3	0	theme	EV	243:244	arg1	composition					246:256	EV composition	243:256	EV composition	243:256	EV composition is complex and includes proteins, glycans, pigments, and RNA.
30918062	18	1	theme	fungal	2413:2418	arg1	species					2420:2426	fungal species	2413:2426	fungal species	2413:2426	In this study, we described the RNA content in EVs produced by two isolates of Histoplasma capsulatum Our results add this important pathogen to the current short list of fungal species with the ability to use EVs for the extracellular release of RNA.
30918062	7	2	theme	G217B	689:693	arg1	strain					695:700	the G217B strain	685:700	the G217B strain	685:700	In this set of RNA classes, 93 transcripts were enriched in EVs from the G217B strain, whereas 31 were enriched in EVs produced by the G186AR strain.
30918062	13	3	from	present	1489:1495	arg1	EVs					1514:1516	H. capsulatum EVs	1500:1516	H. capsulatum EVs	1500:1516	Taking the results together, our study provided information about the RNA molecules present in H. capsulatum EVs and about the differences in composition between the strains.
30918062	17	4	theme	role	2219:2222	arg1	understanding					2196:2208	the understanding	2192:2208	the understanding of their role during infection	2192:2239	Improved knowledge of EV-associated RNA will contribute to the understanding of their role during infection.
30918062	2	5	theme	bilayered	155:163	arg1	compartments					165:176	These lipid bilayered compartments	143:176	These lipid bilayered compartments	143:176	These lipid bilayered compartments play essential roles in cellular communication and pathogenesis.
30918062	13	6	theme	present	1489:1495	arg1	molecules					1479:1487	the RNA molecules	1471:1487	the RNA molecules present in H. capsulatum EVs	1471:1516	Taking the results together, our study provided information about the RNA molecules present in H. capsulatum EVs and about the differences in composition between the strains.
30918062	13	7	from	differences	1532:1542	arg1	composition					1547:1557	composition	1547:1557	composition	1547:1557	Taking the results together, our study provided information about the RNA molecules present in H. capsulatum EVs and about the differences in composition between the strains.
30918062	11	8	theme	molecules	1253:1261	arg1	occurrence					1217:1226	the occurrence	1213:1226	the occurrence of microRNA (miRNA)-like molecules in fungal EVs	1213:1275	Half of these fragments aligned to the reverse strand of the transcript, suggesting the occurrence of microRNA (miRNA)-like molecules in fungal EVs.
30918062	13	9	theme	H.	1500:1501	arg1	EVs					1514:1516	H. capsulatum EVs	1500:1516	H. capsulatum EVs	1500:1516	Taking the results together, our study provided information about the RNA molecules present in H. capsulatum EVs and about the differences in composition between the strains.
30918062	6	10	theme	124	573:575	arg1	mRNAs					577:581	124 mRNAs	573:581	124 mRNAs	573:581	A total of 124 mRNAs were identified in both strains.
30918062	12	11	dep	H.	1325:1326	arg1	capsulatum					1328:1337	H. capsulatum	1325:1337	H. capsulatum	1325:1337	We also compared the transcriptome profiles of H. capsulatum with the RNA composition of EVs, and no correlation was observed.
30918062	13	12	theme	RNA	1475:1477	arg1	molecules					1479:1487	the RNA molecules	1471:1487	the RNA molecules present in H. capsulatum EVs	1471:1516	Taking the results together, our study provided information about the RNA molecules present in H. capsulatum EVs and about the differences in composition between the strains.
30918062	14	13	theme	important	1836:1844	arg1	roles					1846:1850	important roles	1836:1850	important roles	1836:1850	In addition, we found no correlation between the most highly expressed transcripts in the cell and their presence in the EVs, reinforcing the idea that the RNAs were directed to the EVs by a regulated mechanism.IMPORTANCE Extracellular vesicles (EVs) play important roles in cellular communication and pathogenesis.
30918062	11	14	theme	fungal	1266:1271	arg1	EVs					1273:1275	fungal EVs	1266:1275	fungal EVs	1266:1275	Half of these fragments aligned to the reverse strand of the transcript, suggesting the occurrence of microRNA (miRNA)-like molecules in fungal EVs.
30918062	5	15	theme	important	518:526	arg1	capsulatum					503:512	Histoplasma capsulatum	491:512	Histoplasma capsulatum	491:512	Here we describe the RNA content in EVs produced by the G186AR and G217B strains of Histoplasma capsulatum, an important human-pathogenic fungal pathogen.
30918062	5	15	theme	important	518:526	arg1	pathogen					552:559	an important human-pathogenic fungal pathogen	515:559	an important human-pathogenic fungal pathogen	515:559	Here we describe the RNA content in EVs produced by the G186AR and G217B strains of Histoplasma capsulatum, an important human-pathogenic fungal pathogen.
30918062	7	16	from	strain	695:700	arg1	EVs					676:678	EVs	676:678	EVs from the G217B strain	676:700	In this set of RNA classes, 93 transcripts were enriched in EVs from the G217B strain, whereas 31 were enriched in EVs produced by the G186AR strain.
30918062	16	17	theme	pathogenic	2027:2036	arg1	fungi					2038:2042	pathogenic fungi	2027:2042	pathogenic fungi	2027:2042	EV-associated RNA classes have recently been described in pathogenic fungi; however, only a few reports of studies describing the RNAs in fungal EVs are available.
30918062	5	18	theme	fungal	545:550	arg1	capsulatum					503:512	Histoplasma capsulatum	491:512	Histoplasma capsulatum	491:512	Here we describe the RNA content in EVs produced by the G186AR and G217B strains of Histoplasma capsulatum, an important human-pathogenic fungal pathogen.
30918062	5	18	theme	fungal	545:550	arg1	pathogen					552:559	an important human-pathogenic fungal pathogen	515:559	an important human-pathogenic fungal pathogen	515:559	Here we describe the RNA content in EVs produced by the G186AR and G217B strains of Histoplasma capsulatum, an important human-pathogenic fungal pathogen.
30918062	17	19	theme	EV-associated	2155:2167	arg1	RNA					2169:2171	EV-associated RNA	2155:2171	EV-associated RNA	2155:2171	Improved knowledge of EV-associated RNA will contribute to the understanding of their role during infection.
30918062	7	20	theme	RNA	631:633	arg1	classes					635:641	RNA classes	631:641	RNA classes	631:641	In this set of RNA classes, 93 transcripts were enriched in EVs from the G217B strain, whereas 31 were enriched in EVs produced by the G186AR strain.
30918062	5	21	theme	Histoplasma	491:501	arg1	capsulatum					503:512	Histoplasma capsulatum	491:512	Histoplasma capsulatum	491:512	Here we describe the RNA content in EVs produced by the G186AR and G217B strains of Histoplasma capsulatum, an important human-pathogenic fungal pathogen.
30918062	5	21	theme	Histoplasma	491:501	arg1	pathogen					552:559	an important human-pathogenic fungal pathogen	515:559	an important human-pathogenic fungal pathogen	515:559	Here we describe the RNA content in EVs produced by the G186AR and G217B strains of Histoplasma capsulatum, an important human-pathogenic fungal pathogen.
30918062	13	22	attach	present	1489:1495	arg2	molecules					1479:1487	the RNA molecules	1471:1487	the RNA molecules present in H. capsulatum EVs	1471:1516	Taking the results together, our study provided information about the RNA molecules present in H. capsulatum EVs and about the differences in composition between the strains.
30918062	13	22	attach	present	1489:1495	arg1	EVs					1514:1516	H. capsulatum EVs	1500:1516	H. capsulatum EVs	1500:1516	Taking the results together, our study provided information about the RNA molecules present in H. capsulatum EVs and about the differences in composition between the strains.
30918062	0	23	theme	RNA	31:33	arg1	Release					35:41	Extracellular Vesicle-Mediated RNA Release	0:41	Extracellular Vesicle-Mediated RNA Release in Histoplasma capsulatum.	0:68	Extracellular Vesicle-Mediated RNA Release in Histoplasma capsulatum.
30918062	11	24	theme	transcript	1190:1199	arg1	strand					1176:1181	the reverse strand	1164:1181	the reverse strand of the transcript	1164:1199	Half of these fragments aligned to the reverse strand of the transcript, suggesting the occurrence of microRNA (miRNA)-like molecules in fungal EVs.
30918062	18	25	with	list	2405:2408	arg1	ability					2437:2443	the ability to use EVs for the extracellular release of RNA	2433:2491	the ability to use EVs for the extracellular release of RNA	2433:2491	In this study, we described the RNA content in EVs produced by two isolates of Histoplasma capsulatum Our results add this important pathogen to the current short list of fungal species with the ability to use EVs for the extracellular release of RNA.
30918062	9	26	dep	40	920:921	arg1	to					917:918	to	917:918	to	917:918	We also identified short fragments (25 to 40 nucleotides in length) that were strain specific, with a greater number identified in EVs produced by the G217B strain.
30918062	1	27	theme	extracellular	113:125	arg1	EVs					137:139	EVs	137:139	EVs	137:139	Eukaryotic cells, including fungi, release extracellular vesicles (EVs).
30918062	1	27	theme	extracellular	113:125	arg1	vesicles					127:134	extracellular vesicles	113:134	extracellular vesicles (EVs)	113:140	Eukaryotic cells, including fungi, release extracellular vesicles (EVs).
30918062	8	28	theme	strain-specific	812:826	arg1	properties					828:837	important strain-specific properties	802:837	important strain-specific properties	802:837	This result suggests that there are important strain-specific properties in the mRNA composition of fungal EVs.
30918062	4	29	with	RNAs	320:323	arg1	roles					339:343	putative roles	330:343	putative roles in pathogenesis	330:359	RNAs with putative roles in pathogenesis have been described in EVs produced by fungi.
30918062	16	30	theme	studies	2076:2082	arg1	reports					2065:2071	only a few reports	2054:2071	only a few reports of studies describing the RNAs in fungal EVs	2054:2116	EV-associated RNA classes have recently been described in pathogenic fungi; however, only a few reports of studies describing the RNAs in fungal EVs are available.
30918062	16	31	theme	RNA	1983:1985	arg1	classes					1987:1993	EV-associated RNA classes	1969:1993	EV-associated RNA classes	1969:1993	EV-associated RNA classes have recently been described in pathogenic fungi; however, only a few reports of studies describing the RNAs in fungal EVs are available.
30918062	0	32	theme	Extracellular	0:12	arg1	Release					35:41	Extracellular Vesicle-Mediated RNA Release	0:41	Extracellular Vesicle-Mediated RNA Release in Histoplasma capsulatum.	0:68	Extracellular Vesicle-Mediated RNA Release in Histoplasma capsulatum.
30918062	7	33	theme	classes	635:641	arg1	set					624:626	this set	619:626	this set of RNA classes	619:641	In this set of RNA classes, 93 transcripts were enriched in EVs from the G217B strain, whereas 31 were enriched in EVs produced by the G186AR strain.
30918062	7	33	theme	classes	635:641	arg1	classes					635:641	RNA classes	631:641	RNA classes	631:641	In this set of RNA classes, 93 transcripts were enriched in EVs from the G217B strain, whereas 31 were enriched in EVs produced by the G186AR strain.
30918062	8	34	theme	mRNA	846:849	arg1	composition					851:861	the mRNA composition	842:861	the mRNA composition of fungal EVs	842:875	This result suggests that there are important strain-specific properties in the mRNA composition of fungal EVs.
30918062	11	35	theme	fragments	1143:1151	arg1	Half					1129:1132	Half	1129:1132	Half of these fragments	1129:1151	Half of these fragments aligned to the reverse strand of the transcript, suggesting the occurrence of microRNA (miRNA)-like molecules in fungal EVs.
30918062	14	36	from	presence	1685:1692	arg1	cell					1670:1673	the cell	1666:1673	the cell	1666:1673	In addition, we found no correlation between the most highly expressed transcripts in the cell and their presence in the EVs, reinforcing the idea that the RNAs were directed to the EVs by a regulated mechanism.IMPORTANCE Extracellular vesicles (EVs) play important roles in cellular communication and pathogenesis.
30918062	14	36	from	presence	1685:1692	arg1	EVs					1701:1703	the EVs	1697:1703	the EVs	1697:1703	In addition, we found no correlation between the most highly expressed transcripts in the cell and their presence in the EVs, reinforcing the idea that the RNAs were directed to the EVs by a regulated mechanism.IMPORTANCE Extracellular vesicles (EVs) play important roles in cellular communication and pathogenesis.
30918062	11	37	from	occurrence	1217:1226	arg1	EVs					1273:1275	fungal EVs	1266:1275	fungal EVs	1266:1275	Half of these fragments aligned to the reverse strand of the transcript, suggesting the occurrence of microRNA (miRNA)-like molecules in fungal EVs.
30918062	5	38	from	content	432:438	arg1	EVs					443:445	EVs	443:445	EVs produced by the G186AR and G217B strains of Histoplasma capsulatum, an important human-pathogenic fungal pathogen	443:559	Here we describe the RNA content in EVs produced by the G186AR and G217B strains of Histoplasma capsulatum, an important human-pathogenic fungal pathogen.
30918062	18	39	theme	RNA	2274:2276	arg1	content					2278:2284	the RNA content	2270:2284	the RNA content in EVs produced by two isolates of Histoplasma capsulatum Our results add this important pathogen to the current short list of fungal species with the ability to use EVs for the extracellular release of RNA	2270:2491	In this study, we described the RNA content in EVs produced by two isolates of Histoplasma capsulatum Our results add this important pathogen to the current short list of fungal species with the ability to use EVs for the extracellular release of RNA.
30918062	2	40	theme	essential	183:191	arg1	roles					193:197	essential roles	183:197	essential roles	183:197	These lipid bilayered compartments play essential roles in cellular communication and pathogenesis.
30918062	9	41	theme	G217B	1029:1033	arg1	strain					1035:1040	the G217B strain	1025:1040	the G217B strain	1025:1040	We also identified short fragments (25 to 40 nucleotides in length) that were strain specific, with a greater number identified in EVs produced by the G217B strain.
30918062	14	42	from	transcripts	1651:1661	arg1	cell					1670:1673	the cell	1666:1673	the cell	1666:1673	In addition, we found no correlation between the most highly expressed transcripts in the cell and their presence in the EVs, reinforcing the idea that the RNAs were directed to the EVs by a regulated mechanism.IMPORTANCE Extracellular vesicles (EVs) play important roles in cellular communication and pathogenesis.
30918062	14	42	from	transcripts	1651:1661	arg1	EVs					1701:1703	the EVs	1697:1703	the EVs	1697:1703	In addition, we found no correlation between the most highly expressed transcripts in the cell and their presence in the EVs, reinforcing the idea that the RNAs were directed to the EVs by a regulated mechanism.IMPORTANCE Extracellular vesicles (EVs) play important roles in cellular communication and pathogenesis.
30918062	10	43	theme	enriched	1071:1078	arg1	processes					1080:1088	the most highly enriched processes	1055:1088	the most highly enriched processes	1055:1088	Remarkably, the most highly enriched processes were stress responses and translation.
30918062	10	43	theme	enriched	1071:1078	arg1	responses					1102:1110	stress responses	1095:1110	stress responses	1095:1110	Remarkably, the most highly enriched processes were stress responses and translation.
30918062	8	44	theme	EVs	873:875	arg1	composition					851:861	the mRNA composition	842:861	the mRNA composition of fungal EVs	842:875	This result suggests that there are important strain-specific properties in the mRNA composition of fungal EVs.
30918062	11	45	theme	reverse	1168:1174	arg1	strand					1176:1181	the reverse strand	1164:1181	the reverse strand of the transcript	1164:1199	Half of these fragments aligned to the reverse strand of the transcript, suggesting the occurrence of microRNA (miRNA)-like molecules in fungal EVs.
30918062	9	46	dep	fragments	903:911	arg1	nucleotides					923:933	25 to 40 nucleotides	914:933	25 to 40 nucleotides in length	914:943	We also identified short fragments (25 to 40 nucleotides in length) that were strain specific, with a greater number identified in EVs produced by the G217B strain.
30918062	12	47	with	profiles	1313:1320	arg1	composition					1352:1362	the RNA composition	1344:1362	the RNA composition of EVs	1344:1369	We also compared the transcriptome profiles of H. capsulatum with the RNA composition of EVs, and no correlation was observed.
30918062	14	48	theme	expressed	1641:1649	arg1	transcripts					1651:1661	the most highly expressed transcripts	1625:1661	the most highly expressed transcripts in the cell	1625:1673	In addition, we found no correlation between the most highly expressed transcripts in the cell and their presence in the EVs, reinforcing the idea that the RNAs were directed to the EVs by a regulated mechanism.IMPORTANCE Extracellular vesicles (EVs) play important roles in cellular communication and pathogenesis.
30918062	14	49	theme	Extracellular	1802:1814	arg1	EVs					1826:1828	EVs	1826:1828	EVs	1826:1828	In addition, we found no correlation between the most highly expressed transcripts in the cell and their presence in the EVs, reinforcing the idea that the RNAs were directed to the EVs by a regulated mechanism.IMPORTANCE Extracellular vesicles (EVs) play important roles in cellular communication and pathogenesis.
30918062	14	49	theme	Extracellular	1802:1814	arg1	vesicles					1816:1823	Extracellular vesicles	1802:1823	Extracellular vesicles (EVs)	1802:1829	In addition, we found no correlation between the most highly expressed transcripts in the cell and their presence in the EVs, reinforcing the idea that the RNAs were directed to the EVs by a regulated mechanism.IMPORTANCE Extracellular vesicles (EVs) play important roles in cellular communication and pathogenesis.
30918062	15	50	from	molecules	1904:1912	arg1	EVs					1917:1919	EVs	1917:1919	EVs	1917:1919	The RNA molecules in EVs have been implicated in a variety of processes.
30918062	15	51	theme	RNA	1900:1902	arg1	molecules					1904:1912	The RNA molecules	1896:1912	The RNA molecules in EVs	1896:1919	The RNA molecules in EVs have been implicated in a variety of processes.
30918062	12	52	theme	RNA	1348:1350	arg1	composition					1352:1362	the RNA composition	1344:1362	the RNA composition of EVs	1344:1369	We also compared the transcriptome profiles of H. capsulatum with the RNA composition of EVs, and no correlation was observed.
30918062	14	53	theme	regulated	1771:1779	arg1	mechanism.IMPORTANCE					1781:1800	a regulated mechanism.IMPORTANCE	1769:1800	a regulated mechanism.IMPORTANCE Extracellular vesicles (EVs) play important roles in cellular communication and pathogenesis	1769:1893	In addition, we found no correlation between the most highly expressed transcripts in the cell and their presence in the EVs, reinforcing the idea that the RNAs were directed to the EVs by a regulated mechanism.IMPORTANCE Extracellular vesicles (EVs) play important roles in cellular communication and pathogenesis.
30918062	4	54	theme	putative	330:337	arg1	roles					339:343	putative roles	330:343	putative roles in pathogenesis	330:359	RNAs with putative roles in pathogenesis have been described in EVs produced by fungi.
30918062	18	55	theme	species	2420:2426	arg1	list					2405:2408	the current short list	2387:2408	the current short list of fungal species with the ability to use EVs for the extracellular release of RNA	2387:2491	In this study, we described the RNA content in EVs produced by two isolates of Histoplasma capsulatum Our results add this important pathogen to the current short list of fungal species with the ability to use EVs for the extracellular release of RNA.
30918062	18	56	theme	current	2391:2397	arg1	list					2405:2408	the current short list	2387:2408	the current short list of fungal species with the ability to use EVs for the extracellular release of RNA	2387:2491	In this study, we described the RNA content in EVs produced by two isolates of Histoplasma capsulatum Our results add this important pathogen to the current short list of fungal species with the ability to use EVs for the extracellular release of RNA.
30918062	13	57	theme	capsulatum	1503:1512	arg1	EVs					1514:1516	H. capsulatum EVs	1500:1516	H. capsulatum EVs	1500:1516	Taking the results together, our study provided information about the RNA molecules present in H. capsulatum EVs and about the differences in composition between the strains.
30918062	18	58	theme	capsulatum	2333:2342	arg1	isolates					2309:2316	two isolates	2305:2316	two isolates of Histoplasma capsulatum Our results add this important pathogen to the current short list of fungal species with the ability to use EVs for the extracellular release of RNA	2305:2491	In this study, we described the RNA content in EVs produced by two isolates of Histoplasma capsulatum Our results add this important pathogen to the current short list of fungal species with the ability to use EVs for the extracellular release of RNA.
30918062	12	59	theme	H.	1325:1326	arg1	profiles					1313:1320	the transcriptome profiles	1295:1320	the transcriptome profiles of H. capsulatum with the RNA composition of EVs	1295:1369	We also compared the transcriptome profiles of H. capsulatum with the RNA composition of EVs, and no correlation was observed.
30918062	9	60	theme	short	897:901	arg1	fragments					903:911	short fragments	897:911	short fragments (25 to 40 nucleotides in length) that were strain specific, with a greater number identified in EVs produced by the G217B strain	897:1040	We also identified short fragments (25 to 40 nucleotides in length) that were strain specific, with a greater number identified in EVs produced by the G217B strain.
30918062	2	61	theme	lipid	149:153	arg1	compartments					165:176	These lipid bilayered compartments	143:176	These lipid bilayered compartments	143:176	These lipid bilayered compartments play essential roles in cellular communication and pathogenesis.
30918062	5	62	theme	G217B	474:478	arg1	strains					480:486	the G186AR and G217B strains	459:486	the G186AR and G217B strains of Histoplasma capsulatum, an important human-pathogenic fungal pathogen	459:559	Here we describe the RNA content in EVs produced by the G186AR and G217B strains of Histoplasma capsulatum, an important human-pathogenic fungal pathogen.
30918062	12	63	theme	transcriptome	1299:1311	arg1	profiles					1313:1320	the transcriptome profiles	1295:1320	the transcriptome profiles of H. capsulatum with the RNA composition of EVs	1295:1369	We also compared the transcriptome profiles of H. capsulatum with the RNA composition of EVs, and no correlation was observed.
30918062	6	64	theme	mRNAs	577:581	arg1	total					564:568	A total	562:568	A total of 124 mRNAs	562:581	A total of 124 mRNAs were identified in both strains.
30918062	14	65	dep	mechanism.IMPORTANCE	1781:1800	arg1	play					1831:1834	play	1831:1834	play important roles in cellular communication and pathogenesis	1831:1893	In addition, we found no correlation between the most highly expressed transcripts in the cell and their presence in the EVs, reinforcing the idea that the RNAs were directed to the EVs by a regulated mechanism.IMPORTANCE Extracellular vesicles (EVs) play important roles in cellular communication and pathogenesis.
30918062	5	66	theme	G186AR	463:468	arg1	strains					480:486	the G186AR and G217B strains	459:486	the G186AR and G217B strains of Histoplasma capsulatum, an important human-pathogenic fungal pathogen	459:559	Here we describe the RNA content in EVs produced by the G186AR and G217B strains of Histoplasma capsulatum, an important human-pathogenic fungal pathogen.
30918062	18	67	dep	isolates	2309:2316	arg1	add					2356:2358	add	2356:2358	add this important pathogen to the current short list of fungal species with the ability to use EVs for the extracellular release of RNA	2356:2491	In this study, we described the RNA content in EVs produced by two isolates of Histoplasma capsulatum Our results add this important pathogen to the current short list of fungal species with the ability to use EVs for the extracellular release of RNA.
30918062	17	68	theme	Improved	2133:2140	arg1	knowledge					2142:2150	Improved knowledge	2133:2150	Improved knowledge of EV-associated RNA	2133:2171	Improved knowledge of EV-associated RNA will contribute to the understanding of their role during infection.
30918062	11	69	theme	-like	1247:1251	arg1	molecules					1253:1261	microRNA (miRNA)-like molecules	1231:1261	microRNA (miRNA)-like molecules	1231:1261	Half of these fragments aligned to the reverse strand of the transcript, suggesting the occurrence of microRNA (miRNA)-like molecules in fungal EVs.
30918062	5	70	theme	human-pathogenic	528:543	arg1	capsulatum					503:512	Histoplasma capsulatum	491:512	Histoplasma capsulatum	491:512	Here we describe the RNA content in EVs produced by the G186AR and G217B strains of Histoplasma capsulatum, an important human-pathogenic fungal pathogen.
30918062	5	70	theme	human-pathogenic	528:543	arg1	pathogen					552:559	an important human-pathogenic fungal pathogen	515:559	an important human-pathogenic fungal pathogen	515:559	Here we describe the RNA content in EVs produced by the G186AR and G217B strains of Histoplasma capsulatum, an important human-pathogenic fungal pathogen.
30918062	4	71	from	roles	339:343	arg1	pathogenesis					348:359	pathogenesis	348:359	pathogenesis	348:359	RNAs with putative roles in pathogenesis have been described in EVs produced by fungi.
30918062	17	72	theme	RNA	2169:2171	arg1	knowledge					2142:2150	Improved knowledge	2133:2150	Improved knowledge of EV-associated RNA	2133:2171	Improved knowledge of EV-associated RNA will contribute to the understanding of their role during infection.
30918062	9	73	theme	greater	980:986	arg1	number					988:993	a greater number	978:993	a greater number identified in EVs produced by the G217B strain	978:1040	We also identified short fragments (25 to 40 nucleotides in length) that were strain specific, with a greater number identified in EVs produced by the G217B strain.
30918062	5	74	theme	capsulatum	503:512	arg1	strains					480:486	the G186AR and G217B strains	459:486	the G186AR and G217B strains of Histoplasma capsulatum, an important human-pathogenic fungal pathogen	459:559	Here we describe the RNA content in EVs produced by the G186AR and G217B strains of Histoplasma capsulatum, an important human-pathogenic fungal pathogen.
30918062	7	75	dep	enriched	664:671	arg1	whereas					703:709	whereas	703:709	whereas	703:709	In this set of RNA classes, 93 transcripts were enriched in EVs from the G217B strain, whereas 31 were enriched in EVs produced by the G186AR strain.
30918062	0	76	from	Release	35:41	arg1	capsulatum					58:67	Histoplasma capsulatum	46:67	Histoplasma capsulatum	46:67	Extracellular Vesicle-Mediated RNA Release in Histoplasma capsulatum.
30918062	18	77	theme	important	2365:2373	arg1	pathogen					2375:2382	this important pathogen	2360:2382	this important pathogen	2360:2382	In this study, we described the RNA content in EVs produced by two isolates of Histoplasma capsulatum Our results add this important pathogen to the current short list of fungal species with the ability to use EVs for the extracellular release of RNA.
30918062	9	78	from	nucleotides	923:933	arg1	length					938:943	length	938:943	length	938:943	We also identified short fragments (25 to 40 nucleotides in length) that were strain specific, with a greater number identified in EVs produced by the G217B strain.
30918062	1	79	theme	Eukaryotic	70:79	arg1	cells					81:85	Eukaryotic cells	70:85	Eukaryotic cells	70:85	Eukaryotic cells, including fungi, release extracellular vesicles (EVs).
30918062	1	79	theme	Eukaryotic	70:79	arg1	fungi					98:102	fungi	98:102	fungi	98:102	Eukaryotic cells, including fungi, release extracellular vesicles (EVs).
30918062	9	80	with	specific	963:970	arg1	number					988:993	a greater number	978:993	a greater number identified in EVs produced by the G217B strain	978:1040	We also identified short fragments (25 to 40 nucleotides in length) that were strain specific, with a greater number identified in EVs produced by the G217B strain.
30918062	8	81	theme	important	802:810	arg1	properties					828:837	important strain-specific properties	802:837	important strain-specific properties	802:837	This result suggests that there are important strain-specific properties in the mRNA composition of fungal EVs.
30918062	0	82	theme	Vesicle-Mediated	14:29	arg1	Release					35:41	Extracellular Vesicle-Mediated RNA Release	0:41	Extracellular Vesicle-Mediated RNA Release in Histoplasma capsulatum.	0:68	Extracellular Vesicle-Mediated RNA Release in Histoplasma capsulatum.
30918062	14	83	theme	cellular	1855:1862	arg1	communication					1864:1876	cellular communication	1855:1876	cellular communication	1855:1876	In addition, we found no correlation between the most highly expressed transcripts in the cell and their presence in the EVs, reinforcing the idea that the RNAs were directed to the EVs by a regulated mechanism.IMPORTANCE Extracellular vesicles (EVs) play important roles in cellular communication and pathogenesis.
30918062	16	84	theme	EV-associated	1969:1981	arg1	classes					1987:1993	EV-associated RNA classes	1969:1993	EV-associated RNA classes	1969:1993	EV-associated RNA classes have recently been described in pathogenic fungi; however, only a few reports of studies describing the RNAs in fungal EVs are available.
30918062	16	85	theme	fungal	2107:2112	arg1	EVs					2114:2116	fungal EVs	2107:2116	fungal EVs	2107:2116	EV-associated RNA classes have recently been described in pathogenic fungi; however, only a few reports of studies describing the RNAs in fungal EVs are available.
30918062	7	86	theme	G186AR	751:756	arg1	strain					758:763	the G186AR strain	747:763	the G186AR strain	747:763	In this set of RNA classes, 93 transcripts were enriched in EVs from the G217B strain, whereas 31 were enriched in EVs produced by the G186AR strain.
30918062	18	87	theme	RNA	2489:2491	arg1	release					2478:2484	the extracellular release	2460:2484	the extracellular release of RNA	2460:2491	In this study, we described the RNA content in EVs produced by two isolates of Histoplasma capsulatum Our results add this important pathogen to the current short list of fungal species with the ability to use EVs for the extracellular release of RNA.
30918062	0	88	theme	Histoplasma	46:56	arg1	capsulatum					58:67	Histoplasma capsulatum	46:67	Histoplasma capsulatum	46:67	Extracellular Vesicle-Mediated RNA Release in Histoplasma capsulatum.
30918062	18	89	theme	extracellular	2464:2476	arg1	release					2478:2484	the extracellular release	2460:2484	the extracellular release of RNA	2460:2491	In this study, we described the RNA content in EVs produced by two isolates of Histoplasma capsulatum Our results add this important pathogen to the current short list of fungal species with the ability to use EVs for the extracellular release of RNA.
30918062	16	90	from	RNAs	2099:2102	arg1	EVs					2114:2116	fungal EVs	2107:2116	fungal EVs	2107:2116	EV-associated RNA classes have recently been described in pathogenic fungi; however, only a few reports of studies describing the RNAs in fungal EVs are available.
30918062	15	91	theme	processes	1958:1966	arg1	variety					1947:1953	a variety	1945:1953	a variety of processes	1945:1966	The RNA molecules in EVs have been implicated in a variety of processes.
30918062	15	91	theme	processes	1958:1966	arg1	processes					1958:1966	processes	1958:1966	processes	1958:1966	The RNA molecules in EVs have been implicated in a variety of processes.
30918062	8	92	theme	fungal	866:871	arg1	EVs					873:875	fungal EVs	866:875	fungal EVs	866:875	This result suggests that there are important strain-specific properties in the mRNA composition of fungal EVs.
30918062	5	93	theme	RNA	428:430	arg1	content					432:438	the RNA content	424:438	the RNA content in EVs produced by the G186AR and G217B strains of Histoplasma capsulatum, an important human-pathogenic fungal pathogen	424:559	Here we describe the RNA content in EVs produced by the G186AR and G217B strains of Histoplasma capsulatum, an important human-pathogenic fungal pathogen.
30918062	12	94	theme	EVs	1367:1369	arg1	composition					1352:1362	the RNA composition	1344:1362	the RNA composition of EVs	1344:1369	We also compared the transcriptome profiles of H. capsulatum with the RNA composition of EVs, and no correlation was observed.
30918062	2	95	theme	cellular	202:209	arg1	communication					211:223	cellular communication	202:223	cellular communication	202:223	These lipid bilayered compartments play essential roles in cellular communication and pathogenesis.
30918062	10	96	theme	stress	1095:1100	arg1	processes					1080:1088	the most highly enriched processes	1055:1088	the most highly enriched processes	1055:1088	Remarkably, the most highly enriched processes were stress responses and translation.
30918062	10	96	theme	stress	1095:1100	arg1	responses					1102:1110	stress responses	1095:1110	stress responses	1095:1110	Remarkably, the most highly enriched processes were stress responses and translation.
30918062	18	97	theme	short	2399:2403	arg1	list					2405:2408	the current short list	2387:2408	the current short list of fungal species with the ability to use EVs for the extracellular release of RNA	2387:2491	In this study, we described the RNA content in EVs produced by two isolates of Histoplasma capsulatum Our results add this important pathogen to the current short list of fungal species with the ability to use EVs for the extracellular release of RNA.
30918062	18	98	from	content	2278:2284	arg1	EVs					2289:2291	EVs	2289:2291	EVs produced by two isolates of Histoplasma capsulatum Our results add this important pathogen to the current short list of fungal species with the ability to use EVs for the extracellular release of RNA	2289:2491	In this study, we described the RNA content in EVs produced by two isolates of Histoplasma capsulatum Our results add this important pathogen to the current short list of fungal species with the ability to use EVs for the extracellular release of RNA.
30918062	13	99	from	EVs	1514:1516	arg1	present					1489:1495	present	1489:1495	present	1489:1495	Taking the results together, our study provided information about the RNA molecules present in H. capsulatum EVs and about the differences in composition between the strains.
29329275	1	0	theme	thermal	285:291	arg1	stability					293:301	thermal stability	285:301	thermal stability	285:301	The aim of this study is to analyze the properties of a series of polysaccharide composite films, such as apparent density, color, the presence of functional groups, morphology, and thermal stability, as well as the correlation between them and their antimicrobial and optical properties.
29329275	6	1	theme	other	1210:1214	arg1	hand					1216:1219	the other hand	1206:1219	the other hand	1206:1219	On the other hand, the TGA analysis showed that the type of extract influences the total weight loss.
29329275	4	2	theme	acid	909:912	arg1	curve					926:930	a gallic acid calibration curve	900:930	a gallic acid calibration curve	900:930	Due to this fact, the antioxidants were extracted and identified by spectroscopic analysis; and they were also quantified using the Folin-Denis method and a gallic acid calibration curve, which confirmed the presence of natural antioxidants in the films.
29329275	2	3	from	pitaya	508:513	arg1	betalains					479:487	betalains	479:487	betalains (from beetroot and pitaya)	479:514	Natural antioxidants such as anthocyanins (from cranberry; blueberry and pomegranate); betalains (from beetroot and pitaya); resveratrol (from grape); and thymol and carvacrol (from oregano) were added to the films.
29329275	2	3	from	pitaya	508:513	arg1	carvacrol					558:566	carvacrol	558:566	carvacrol	558:566	Natural antioxidants such as anthocyanins (from cranberry; blueberry and pomegranate); betalains (from beetroot and pitaya); resveratrol (from grape); and thymol and carvacrol (from oregano) were added to the films.
29329275	2	3	from	pitaya	508:513	arg1	anthocyanins					421:432	anthocyanins	421:432	anthocyanins (from cranberry; blueberry and pomegranate)	421:476	Natural antioxidants such as anthocyanins (from cranberry; blueberry and pomegranate); betalains (from beetroot and pitaya); resveratrol (from grape); and thymol and carvacrol (from oregano) were added to the films.
29329275	2	3	from	pitaya	508:513	arg1	resveratrol					517:527	resveratrol	517:527	resveratrol (from grape)	517:540	Natural antioxidants such as anthocyanins (from cranberry; blueberry and pomegranate); betalains (from beetroot and pitaya); resveratrol (from grape); and thymol and carvacrol (from oregano) were added to the films.
29329275	2	3	from	pitaya	508:513	arg1	thymol					547:552	thymol	547:552	thymol	547:552	Natural antioxidants such as anthocyanins (from cranberry; blueberry and pomegranate); betalains (from beetroot and pitaya); resveratrol (from grape); and thymol and carvacrol (from oregano) were added to the films.
29329275	0	4	theme	Morphological	65:77	arg1	Properties					91:100	Physical, Chemical, Morphological and Thermal Properties	45:100	Chitosan-Starch Films with Natural Extracts: Physical, Chemical, Morphological and Thermal Properties.	0:101	Chitosan-Starch Films with Natural Extracts: Physical, Chemical, Morphological and Thermal Properties.
29329275	7	5	theme	chitosan-starch	1410:1424	arg1	preparation					1431:1441	chitosan-starch film preparation	1410:1441	chitosan-starch film preparation	1410:1441	The overall interpretation of the results suggests that the use of natural antioxidants as additives for chitosan-starch film preparation has a prominent impact on most of the critical properties that are decisive in making them suitable for food-packing applications.
29329275	6	6	theme	total	1286:1290	arg1	loss					1299:1302	the total weight loss	1282:1302	the total weight loss	1282:1302	On the other hand, the TGA analysis showed that the type of extract influences the total weight loss.
29329275	3	7	theme	extract	717:723	arg1	content					706:712	the low content	698:712	the low content of extract added to the films	698:742	Few changes in the position and intensity of the FTIR spectra bands were observed despite the low content of extract added to the films.
29329275	4	8	theme	spectroscopic	813:825	arg1	analysis					827:834	spectroscopic analysis	813:834	spectroscopic analysis	813:834	Due to this fact, the antioxidants were extracted and identified by spectroscopic analysis; and they were also quantified using the Folin-Denis method and a gallic acid calibration curve, which confirmed the presence of natural antioxidants in the films.
29329275	3	9	theme	low	702:704	arg1	content					706:712	the low content	698:712	the low content of extract added to the films	698:742	Few changes in the position and intensity of the FTIR spectra bands were observed despite the low content of extract added to the films.
29329275	0	10	theme	Thermal	83:89	arg1	Properties					91:100	Physical, Chemical, Morphological and Thermal Properties	45:100	Chitosan-Starch Films with Natural Extracts: Physical, Chemical, Morphological and Thermal Properties.	0:101	Chitosan-Starch Films with Natural Extracts: Physical, Chemical, Morphological and Thermal Properties.
29329275	4	11	attach	presence	953:960	arg1	films					993:997	the films	989:997	the films	989:997	Due to this fact, the antioxidants were extracted and identified by spectroscopic analysis; and they were also quantified using the Folin-Denis method and a gallic acid calibration curve, which confirmed the presence of natural antioxidants in the films.
29329275	4	11	attach	presence	953:960	arg2	antioxidants					973:984	natural antioxidants	965:984	natural antioxidants	965:984	Due to this fact, the antioxidants were extracted and identified by spectroscopic analysis; and they were also quantified using the Folin-Denis method and a gallic acid calibration curve, which confirmed the presence of natural antioxidants in the films.
29329275	7	12	contain	has	1443:1445	arg2	impact					1459:1464	a prominent impact	1447:1464	a prominent impact	1447:1464	The overall interpretation of the results suggests that the use of natural antioxidants as additives for chitosan-starch film preparation has a prominent impact on most of the critical properties that are decisive in making them suitable for food-packing applications.
29329275	7	12	contain	has	1443:1445	arg1	use					1365:1367	the use	1361:1367	the use of natural antioxidants as additives for chitosan-starch film preparation	1361:1441	The overall interpretation of the results suggests that the use of natural antioxidants as additives for chitosan-starch film preparation has a prominent impact on most of the critical properties that are decisive in making them suitable for food-packing applications.
29329275	7	13	theme	antioxidants	1380:1391	arg1	use					1365:1367	the use	1361:1367	the use of natural antioxidants as additives for chitosan-starch film preparation	1361:1441	The overall interpretation of the results suggests that the use of natural antioxidants as additives for chitosan-starch film preparation has a prominent impact on most of the critical properties that are decisive in making them suitable for food-packing applications.
29329275	6	14	theme	weight	1292:1297	arg1	loss					1299:1302	the total weight loss	1282:1302	the total weight loss	1282:1302	On the other hand, the TGA analysis showed that the type of extract influences the total weight loss.
29329275	3	15	theme	Few	608:610	arg1	changes					612:618	Few changes	608:618	Few changes in the position and intensity of the FTIR spectra bands	608:674	Few changes in the position and intensity of the FTIR spectra bands were observed despite the low content of extract added to the films.
29329275	5	16	contain	has	1068:1070	arg1	presence					1035:1042	the presence	1031:1042	the presence of natural antioxidants	1031:1066	According to the SEM analysis, the presence of natural antioxidants has no influence on the film morphology because the stretch marks and white points that were observed were related to starch presence.
29329275	5	16	contain	has	1068:1070	arg2	influence					1075:1083	no influence	1072:1083	no influence on the film morphology	1072:1106	According to the SEM analysis, the presence of natural antioxidants has no influence on the film morphology because the stretch marks and white points that were observed were related to starch presence.
29329275	0	17	theme	Chitosan-Starch	0:14	arg1	Films					16:20	Chitosan-Starch Films	0:20	Chitosan-Starch Films with Natural Extracts: Physical, Chemical, Morphological and Thermal Properties.	0:101	Chitosan-Starch Films with Natural Extracts: Physical, Chemical, Morphological and Thermal Properties.
29329275	5	18	theme	starch	1186:1191	arg1	presence					1193:1200	starch presence	1186:1200	starch presence	1186:1200	According to the SEM analysis, the presence of natural antioxidants has no influence on the film morphology because the stretch marks and white points that were observed were related to starch presence.
29329275	7	19	theme	natural	1372:1378	arg1	antioxidants					1380:1391	natural antioxidants	1372:1391	natural antioxidants	1372:1391	The overall interpretation of the results suggests that the use of natural antioxidants as additives for chitosan-starch film preparation has a prominent impact on most of the critical properties that are decisive in making them suitable for food-packing applications.
29329275	2	20	theme	Natural	392:398	arg1	resveratrol					517:527	resveratrol	517:527	resveratrol (from grape)	517:540	Natural antioxidants such as anthocyanins (from cranberry; blueberry and pomegranate); betalains (from beetroot and pitaya); resveratrol (from grape); and thymol and carvacrol (from oregano) were added to the films.
29329275	2	20	theme	Natural	392:398	arg1	antioxidants					400:411	Natural antioxidants	392:411	Natural antioxidants such as anthocyanins (from cranberry; blueberry and pomegranate); betalains (from beetroot and pitaya); resveratrol (from grape); and thymol and carvacrol (from oregano)	392:581	Natural antioxidants such as anthocyanins (from cranberry; blueberry and pomegranate); betalains (from beetroot and pitaya); resveratrol (from grape); and thymol and carvacrol (from oregano) were added to the films.
29329275	2	20	theme	Natural	392:398	arg1	anthocyanins					421:432	anthocyanins	421:432	anthocyanins (from cranberry; blueberry and pomegranate)	421:476	Natural antioxidants such as anthocyanins (from cranberry; blueberry and pomegranate); betalains (from beetroot and pitaya); resveratrol (from grape); and thymol and carvacrol (from oregano) were added to the films.
29329275	2	20	theme	Natural	392:398	arg1	betalains					479:487	betalains	479:487	betalains (from beetroot and pitaya)	479:514	Natural antioxidants such as anthocyanins (from cranberry; blueberry and pomegranate); betalains (from beetroot and pitaya); resveratrol (from grape); and thymol and carvacrol (from oregano) were added to the films.
29329275	2	20	theme	Natural	392:398	arg1	thymol					547:552	thymol	547:552	thymol	547:552	Natural antioxidants such as anthocyanins (from cranberry; blueberry and pomegranate); betalains (from beetroot and pitaya); resveratrol (from grape); and thymol and carvacrol (from oregano) were added to the films.
29329275	5	21	theme	natural	1047:1053	arg1	antioxidants					1055:1066	natural antioxidants	1047:1066	natural antioxidants	1047:1066	According to the SEM analysis, the presence of natural antioxidants has no influence on the film morphology because the stretch marks and white points that were observed were related to starch presence.
29329275	4	22	theme	natural	965:971	arg1	antioxidants					973:984	natural antioxidants	965:984	natural antioxidants	965:984	Due to this fact, the antioxidants were extracted and identified by spectroscopic analysis; and they were also quantified using the Folin-Denis method and a gallic acid calibration curve, which confirmed the presence of natural antioxidants in the films.
29329275	1	23	theme	series	159:164	arg1	morphology					269:278	morphology	269:278	morphology	269:278	The aim of this study is to analyze the properties of a series of polysaccharide composite films, such as apparent density, color, the presence of functional groups, morphology, and thermal stability, as well as the correlation between them and their antimicrobial and optical properties.
29329275	1	23	theme	series	159:164	arg1	presence					238:245	the presence	234:245	the presence of functional groups	234:266	The aim of this study is to analyze the properties of a series of polysaccharide composite films, such as apparent density, color, the presence of functional groups, morphology, and thermal stability, as well as the correlation between them and their antimicrobial and optical properties.
29329275	1	23	theme	series	159:164	arg1	density					218:224	apparent density	209:224	apparent density	209:224	The aim of this study is to analyze the properties of a series of polysaccharide composite films, such as apparent density, color, the presence of functional groups, morphology, and thermal stability, as well as the correlation between them and their antimicrobial and optical properties.
29329275	1	23	theme	series	159:164	arg1	stability					293:301	thermal stability	285:301	thermal stability	285:301	The aim of this study is to analyze the properties of a series of polysaccharide composite films, such as apparent density, color, the presence of functional groups, morphology, and thermal stability, as well as the correlation between them and their antimicrobial and optical properties.
29329275	1	23	theme	series	159:164	arg1	properties					143:152	the properties	139:152	the properties of a series of polysaccharide composite films	139:198	The aim of this study is to analyze the properties of a series of polysaccharide composite films, such as apparent density, color, the presence of functional groups, morphology, and thermal stability, as well as the correlation between them and their antimicrobial and optical properties.
29329275	1	23	theme	series	159:164	arg1	color					227:231	color	227:231	color	227:231	The aim of this study is to analyze the properties of a series of polysaccharide composite films, such as apparent density, color, the presence of functional groups, morphology, and thermal stability, as well as the correlation between them and their antimicrobial and optical properties.
29329275	1	23	theme	series	159:164	arg1	correlation					319:329	the correlation	315:329	the correlation between them	315:342	The aim of this study is to analyze the properties of a series of polysaccharide composite films, such as apparent density, color, the presence of functional groups, morphology, and thermal stability, as well as the correlation between them and their antimicrobial and optical properties.
29329275	0	24	theme	Natural	27:33	arg1	Extracts					35:42	Natural Extracts	27:42	Natural Extracts	27:42	Chitosan-Starch Films with Natural Extracts: Physical, Chemical, Morphological and Thermal Properties.
29329275	2	25	from	grape	535:539	arg1	betalains					479:487	betalains	479:487	betalains (from beetroot and pitaya)	479:514	Natural antioxidants such as anthocyanins (from cranberry; blueberry and pomegranate); betalains (from beetroot and pitaya); resveratrol (from grape); and thymol and carvacrol (from oregano) were added to the films.
29329275	2	25	from	grape	535:539	arg1	carvacrol					558:566	carvacrol	558:566	carvacrol	558:566	Natural antioxidants such as anthocyanins (from cranberry; blueberry and pomegranate); betalains (from beetroot and pitaya); resveratrol (from grape); and thymol and carvacrol (from oregano) were added to the films.
29329275	2	25	from	grape	535:539	arg1	anthocyanins					421:432	anthocyanins	421:432	anthocyanins (from cranberry; blueberry and pomegranate)	421:476	Natural antioxidants such as anthocyanins (from cranberry; blueberry and pomegranate); betalains (from beetroot and pitaya); resveratrol (from grape); and thymol and carvacrol (from oregano) were added to the films.
29329275	2	25	from	grape	535:539	arg1	resveratrol					517:527	resveratrol	517:527	resveratrol (from grape)	517:540	Natural antioxidants such as anthocyanins (from cranberry; blueberry and pomegranate); betalains (from beetroot and pitaya); resveratrol (from grape); and thymol and carvacrol (from oregano) were added to the films.
29329275	2	25	from	grape	535:539	arg1	thymol					547:552	thymol	547:552	thymol	547:552	Natural antioxidants such as anthocyanins (from cranberry; blueberry and pomegranate); betalains (from beetroot and pitaya); resveratrol (from grape); and thymol and carvacrol (from oregano) were added to the films.
29329275	2	26	from	cranberry	440:448	arg1	betalains					479:487	betalains	479:487	betalains (from beetroot and pitaya)	479:514	Natural antioxidants such as anthocyanins (from cranberry; blueberry and pomegranate); betalains (from beetroot and pitaya); resveratrol (from grape); and thymol and carvacrol (from oregano) were added to the films.
29329275	2	26	from	cranberry	440:448	arg1	carvacrol					558:566	carvacrol	558:566	carvacrol	558:566	Natural antioxidants such as anthocyanins (from cranberry; blueberry and pomegranate); betalains (from beetroot and pitaya); resveratrol (from grape); and thymol and carvacrol (from oregano) were added to the films.
29329275	2	26	from	cranberry	440:448	arg1	anthocyanins					421:432	anthocyanins	421:432	anthocyanins (from cranberry; blueberry and pomegranate)	421:476	Natural antioxidants such as anthocyanins (from cranberry; blueberry and pomegranate); betalains (from beetroot and pitaya); resveratrol (from grape); and thymol and carvacrol (from oregano) were added to the films.
29329275	2	26	from	cranberry	440:448	arg1	resveratrol					517:527	resveratrol	517:527	resveratrol (from grape)	517:540	Natural antioxidants such as anthocyanins (from cranberry; blueberry and pomegranate); betalains (from beetroot and pitaya); resveratrol (from grape); and thymol and carvacrol (from oregano) were added to the films.
29329275	2	26	from	cranberry	440:448	arg1	thymol					547:552	thymol	547:552	thymol	547:552	Natural antioxidants such as anthocyanins (from cranberry; blueberry and pomegranate); betalains (from beetroot and pitaya); resveratrol (from grape); and thymol and carvacrol (from oregano) were added to the films.
29329275	7	27	theme	critical	1481:1488	arg1	properties					1490:1499	the critical properties	1477:1499	the critical properties that are decisive in making them suitable for food-packing applications	1477:1571	The overall interpretation of the results suggests that the use of natural antioxidants as additives for chitosan-starch film preparation has a prominent impact on most of the critical properties that are decisive in making them suitable for food-packing applications.
29329275	7	27	theme	critical	1481:1488	arg1	decisive					1510:1517	decisive	1510:1517	decisive	1510:1517	The overall interpretation of the results suggests that the use of natural antioxidants as additives for chitosan-starch film preparation has a prominent impact on most of the critical properties that are decisive in making them suitable for food-packing applications.
29329275	1	28	theme	polysaccharide	169:182	arg1	films					194:198	polysaccharide composite films	169:198	polysaccharide composite films	169:198	The aim of this study is to analyze the properties of a series of polysaccharide composite films, such as apparent density, color, the presence of functional groups, morphology, and thermal stability, as well as the correlation between them and their antimicrobial and optical properties.
29329275	1	29	theme	functional	250:259	arg1	groups					261:266	functional groups	250:266	functional groups	250:266	The aim of this study is to analyze the properties of a series of polysaccharide composite films, such as apparent density, color, the presence of functional groups, morphology, and thermal stability, as well as the correlation between them and their antimicrobial and optical properties.
29329275	4	30	theme	antioxidants	973:984	arg1	presence					953:960	the presence	949:960	the presence of natural antioxidants in the films	949:997	Due to this fact, the antioxidants were extracted and identified by spectroscopic analysis; and they were also quantified using the Folin-Denis method and a gallic acid calibration curve, which confirmed the presence of natural antioxidants in the films.
29329275	1	31	theme	composite	184:192	arg1	films					194:198	polysaccharide composite films	169:198	polysaccharide composite films	169:198	The aim of this study is to analyze the properties of a series of polysaccharide composite films, such as apparent density, color, the presence of functional groups, morphology, and thermal stability, as well as the correlation between them and their antimicrobial and optical properties.
29329275	7	32	theme	food-packing	1547:1558	arg1	applications					1560:1571	food-packing applications	1547:1571	food-packing applications	1547:1571	The overall interpretation of the results suggests that the use of natural antioxidants as additives for chitosan-starch film preparation has a prominent impact on most of the critical properties that are decisive in making them suitable for food-packing applications.
29329275	1	33	theme	groups	261:266	arg1	morphology					269:278	morphology	269:278	morphology	269:278	The aim of this study is to analyze the properties of a series of polysaccharide composite films, such as apparent density, color, the presence of functional groups, morphology, and thermal stability, as well as the correlation between them and their antimicrobial and optical properties.
29329275	1	33	theme	groups	261:266	arg1	presence					238:245	the presence	234:245	the presence of functional groups	234:266	The aim of this study is to analyze the properties of a series of polysaccharide composite films, such as apparent density, color, the presence of functional groups, morphology, and thermal stability, as well as the correlation between them and their antimicrobial and optical properties.
29329275	1	33	theme	groups	261:266	arg1	density					218:224	apparent density	209:224	apparent density	209:224	The aim of this study is to analyze the properties of a series of polysaccharide composite films, such as apparent density, color, the presence of functional groups, morphology, and thermal stability, as well as the correlation between them and their antimicrobial and optical properties.
29329275	1	33	theme	groups	261:266	arg1	stability					293:301	thermal stability	285:301	thermal stability	285:301	The aim of this study is to analyze the properties of a series of polysaccharide composite films, such as apparent density, color, the presence of functional groups, morphology, and thermal stability, as well as the correlation between them and their antimicrobial and optical properties.
29329275	1	33	theme	groups	261:266	arg1	properties					143:152	the properties	139:152	the properties of a series of polysaccharide composite films	139:198	The aim of this study is to analyze the properties of a series of polysaccharide composite films, such as apparent density, color, the presence of functional groups, morphology, and thermal stability, as well as the correlation between them and their antimicrobial and optical properties.
29329275	1	33	theme	groups	261:266	arg1	color					227:231	color	227:231	color	227:231	The aim of this study is to analyze the properties of a series of polysaccharide composite films, such as apparent density, color, the presence of functional groups, morphology, and thermal stability, as well as the correlation between them and their antimicrobial and optical properties.
29329275	1	33	theme	groups	261:266	arg1	correlation					319:329	the correlation	315:329	the correlation between them	315:342	The aim of this study is to analyze the properties of a series of polysaccharide composite films, such as apparent density, color, the presence of functional groups, morphology, and thermal stability, as well as the correlation between them and their antimicrobial and optical properties.
29329275	5	34	theme	antioxidants	1055:1066	arg1	presence					1035:1042	the presence	1031:1042	the presence of natural antioxidants	1031:1066	According to the SEM analysis, the presence of natural antioxidants has no influence on the film morphology because the stretch marks and white points that were observed were related to starch presence.
29329275	4	35	theme	gallic	902:907	arg1	curve					926:930	a gallic acid calibration curve	900:930	a gallic acid calibration curve	900:930	Due to this fact, the antioxidants were extracted and identified by spectroscopic analysis; and they were also quantified using the Folin-Denis method and a gallic acid calibration curve, which confirmed the presence of natural antioxidants in the films.
29329275	1	36	theme	films	194:198	arg1	series					159:164	a series	157:164	a series of polysaccharide composite films	157:198	The aim of this study is to analyze the properties of a series of polysaccharide composite films, such as apparent density, color, the presence of functional groups, morphology, and thermal stability, as well as the correlation between them and their antimicrobial and optical properties.
29329275	4	37	theme	Folin-Denis	877:887	arg1	method					889:894	the Folin-Denis method	873:894	the Folin-Denis method	873:894	Due to this fact, the antioxidants were extracted and identified by spectroscopic analysis; and they were also quantified using the Folin-Denis method and a gallic acid calibration curve, which confirmed the presence of natural antioxidants in the films.
29329275	3	38	theme	spectra	662:668	arg1	intensity					640:648	intensity	640:648	intensity	640:648	Few changes in the position and intensity of the FTIR spectra bands were observed despite the low content of extract added to the films.
29329275	3	38	theme	spectra	662:668	arg1	position					627:634	position	627:634	position	627:634	Few changes in the position and intensity of the FTIR spectra bands were observed despite the low content of extract added to the films.
29329275	2	39	dep	cranberry	440:448	arg1	pomegranate					465:475	pomegranate	465:475	pomegranate	465:475	Natural antioxidants such as anthocyanins (from cranberry; blueberry and pomegranate); betalains (from beetroot and pitaya); resveratrol (from grape); and thymol and carvacrol (from oregano) were added to the films.
29329275	2	39	dep	cranberry	440:448	arg1	blueberry					451:459	blueberry	451:459	blueberry	451:459	Natural antioxidants such as anthocyanins (from cranberry; blueberry and pomegranate); betalains (from beetroot and pitaya); resveratrol (from grape); and thymol and carvacrol (from oregano) were added to the films.
29329275	6	40	theme	extract	1263:1269	arg1	type					1255:1258	the type	1251:1258	the type of extract	1251:1269	On the other hand, the TGA analysis showed that the type of extract influences the total weight loss.
29329275	4	41	from	presence	953:960	arg1	films					993:997	the films	989:997	the films	989:997	Due to this fact, the antioxidants were extracted and identified by spectroscopic analysis; and they were also quantified using the Folin-Denis method and a gallic acid calibration curve, which confirmed the presence of natural antioxidants in the films.
29329275	0	42	theme	Physical	45:52	arg1	Properties					91:100	Physical, Chemical, Morphological and Thermal Properties	45:100	Chitosan-Starch Films with Natural Extracts: Physical, Chemical, Morphological and Thermal Properties.	0:101	Chitosan-Starch Films with Natural Extracts: Physical, Chemical, Morphological and Thermal Properties.
29329275	5	43	theme	SEM	1017:1019	arg1	analysis					1021:1028	the SEM analysis	1013:1028	the SEM analysis	1013:1028	According to the SEM analysis, the presence of natural antioxidants has no influence on the film morphology because the stretch marks and white points that were observed were related to starch presence.
29329275	7	44	theme	overall	1309:1315	arg1	interpretation					1317:1330	The overall interpretation	1305:1330	The overall interpretation of the results	1305:1345	The overall interpretation of the results suggests that the use of natural antioxidants as additives for chitosan-starch film preparation has a prominent impact on most of the critical properties that are decisive in making them suitable for food-packing applications.
29329275	7	45	theme	results	1339:1345	arg1	interpretation					1317:1330	The overall interpretation	1305:1330	The overall interpretation of the results	1305:1345	The overall interpretation of the results suggests that the use of natural antioxidants as additives for chitosan-starch film preparation has a prominent impact on most of the critical properties that are decisive in making them suitable for food-packing applications.
29329275	0	46	with	Films	16:20	arg1	Extracts					35:42	Natural Extracts	27:42	Natural Extracts	27:42	Chitosan-Starch Films with Natural Extracts: Physical, Chemical, Morphological and Thermal Properties.
29329275	3	47	theme	FTIR	657:660	arg1	spectra					662:668	the FTIR spectra	653:668	the FTIR spectra	653:668	Few changes in the position and intensity of the FTIR spectra bands were observed despite the low content of extract added to the films.
29329275	5	48	from	influence	1075:1083	arg1	morphology					1097:1106	the film morphology	1088:1106	the film morphology	1088:1106	According to the SEM analysis, the presence of natural antioxidants has no influence on the film morphology because the stretch marks and white points that were observed were related to starch presence.
29329275	3	49	from	changes	612:618	arg1	intensity					640:648	intensity	640:648	intensity	640:648	Few changes in the position and intensity of the FTIR spectra bands were observed despite the low content of extract added to the films.
29329275	3	49	from	changes	612:618	arg1	position					627:634	position	627:634	position	627:634	Few changes in the position and intensity of the FTIR spectra bands were observed despite the low content of extract added to the films.
29329275	7	50	theme	film	1426:1429	arg1	preparation					1431:1441	chitosan-starch film preparation	1410:1441	chitosan-starch film preparation	1410:1441	The overall interpretation of the results suggests that the use of natural antioxidants as additives for chitosan-starch film preparation has a prominent impact on most of the critical properties that are decisive in making them suitable for food-packing applications.
29329275	1	51	theme	study	119:123	arg1	aim					107:109	The aim	103:109	The aim of this study	103:123	The aim of this study is to analyze the properties of a series of polysaccharide composite films, such as apparent density, color, the presence of functional groups, morphology, and thermal stability, as well as the correlation between them and their antimicrobial and optical properties.
29329275	5	52	theme	white	1138:1142	arg1	points					1144:1149	white points	1138:1149	white points that were observed	1138:1168	According to the SEM analysis, the presence of natural antioxidants has no influence on the film morphology because the stretch marks and white points that were observed were related to starch presence.
29329275	6	53	theme	TGA	1226:1228	arg1	analysis					1230:1237	the TGA analysis	1222:1237	the TGA analysis	1222:1237	On the other hand, the TGA analysis showed that the type of extract influences the total weight loss.
29329275	1	54	theme	optical	372:378	arg1	properties					380:389	their antimicrobial and optical properties	348:389	properties	380:389	The aim of this study is to analyze the properties of a series of polysaccharide composite films, such as apparent density, color, the presence of functional groups, morphology, and thermal stability, as well as the correlation between them and their antimicrobial and optical properties.
29329275	0	55	dep	Films	16:20	arg1	Properties					91:100	Physical, Chemical, Morphological and Thermal Properties	45:100	Chitosan-Starch Films with Natural Extracts: Physical, Chemical, Morphological and Thermal Properties.	0:101	Chitosan-Starch Films with Natural Extracts: Physical, Chemical, Morphological and Thermal Properties.
29329275	7	56	theme	prominent	1449:1457	arg1	impact					1459:1464	a prominent impact	1447:1464	a prominent impact	1447:1464	The overall interpretation of the results suggests that the use of natural antioxidants as additives for chitosan-starch film preparation has a prominent impact on most of the critical properties that are decisive in making them suitable for food-packing applications.
29329275	2	57	from	beetroot	495:502	arg1	betalains					479:487	betalains	479:487	betalains (from beetroot and pitaya)	479:514	Natural antioxidants such as anthocyanins (from cranberry; blueberry and pomegranate); betalains (from beetroot and pitaya); resveratrol (from grape); and thymol and carvacrol (from oregano) were added to the films.
29329275	2	57	from	beetroot	495:502	arg1	carvacrol					558:566	carvacrol	558:566	carvacrol	558:566	Natural antioxidants such as anthocyanins (from cranberry; blueberry and pomegranate); betalains (from beetroot and pitaya); resveratrol (from grape); and thymol and carvacrol (from oregano) were added to the films.
29329275	2	57	from	beetroot	495:502	arg1	anthocyanins					421:432	anthocyanins	421:432	anthocyanins (from cranberry; blueberry and pomegranate)	421:476	Natural antioxidants such as anthocyanins (from cranberry; blueberry and pomegranate); betalains (from beetroot and pitaya); resveratrol (from grape); and thymol and carvacrol (from oregano) were added to the films.
29329275	2	57	from	beetroot	495:502	arg1	resveratrol					517:527	resveratrol	517:527	resveratrol (from grape)	517:540	Natural antioxidants such as anthocyanins (from cranberry; blueberry and pomegranate); betalains (from beetroot and pitaya); resveratrol (from grape); and thymol and carvacrol (from oregano) were added to the films.
29329275	2	57	from	beetroot	495:502	arg1	thymol					547:552	thymol	547:552	thymol	547:552	Natural antioxidants such as anthocyanins (from cranberry; blueberry and pomegranate); betalains (from beetroot and pitaya); resveratrol (from grape); and thymol and carvacrol (from oregano) were added to the films.
29329275	0	58	theme	Chemical	55:62	arg1	Properties					91:100	Physical, Chemical, Morphological and Thermal Properties	45:100	Chitosan-Starch Films with Natural Extracts: Physical, Chemical, Morphological and Thermal Properties.	0:101	Chitosan-Starch Films with Natural Extracts: Physical, Chemical, Morphological and Thermal Properties.
29329275	3	59	dep	position	627:634	arg1	bands					670:674	bands	670:674	the position and intensity of the FTIR spectra bands	623:674	Few changes in the position and intensity of the FTIR spectra bands were observed despite the low content of extract added to the films.
29329275	3	59	dep	position	627:634	arg1	the					623:625	the	623:625	the	623:625	Few changes in the position and intensity of the FTIR spectra bands were observed despite the low content of extract added to the films.
29329275	5	60	theme	film	1092:1095	arg1	morphology					1097:1106	the film morphology	1088:1106	the film morphology	1088:1106	According to the SEM analysis, the presence of natural antioxidants has no influence on the film morphology because the stretch marks and white points that were observed were related to starch presence.
29329275	2	61	from	oregano	574:580	arg1	betalains					479:487	betalains	479:487	betalains (from beetroot and pitaya)	479:514	Natural antioxidants such as anthocyanins (from cranberry; blueberry and pomegranate); betalains (from beetroot and pitaya); resveratrol (from grape); and thymol and carvacrol (from oregano) were added to the films.
29329275	2	61	from	oregano	574:580	arg1	carvacrol					558:566	carvacrol	558:566	carvacrol	558:566	Natural antioxidants such as anthocyanins (from cranberry; blueberry and pomegranate); betalains (from beetroot and pitaya); resveratrol (from grape); and thymol and carvacrol (from oregano) were added to the films.
29329275	2	61	from	oregano	574:580	arg1	anthocyanins					421:432	anthocyanins	421:432	anthocyanins (from cranberry; blueberry and pomegranate)	421:476	Natural antioxidants such as anthocyanins (from cranberry; blueberry and pomegranate); betalains (from beetroot and pitaya); resveratrol (from grape); and thymol and carvacrol (from oregano) were added to the films.
29329275	2	61	from	oregano	574:580	arg1	resveratrol					517:527	resveratrol	517:527	resveratrol (from grape)	517:540	Natural antioxidants such as anthocyanins (from cranberry; blueberry and pomegranate); betalains (from beetroot and pitaya); resveratrol (from grape); and thymol and carvacrol (from oregano) were added to the films.
29329275	2	61	from	oregano	574:580	arg1	thymol					547:552	thymol	547:552	thymol	547:552	Natural antioxidants such as anthocyanins (from cranberry; blueberry and pomegranate); betalains (from beetroot and pitaya); resveratrol (from grape); and thymol and carvacrol (from oregano) were added to the films.
29329275	4	62	theme	calibration	914:924	arg1	curve					926:930	a gallic acid calibration curve	900:930	a gallic acid calibration curve	900:930	Due to this fact, the antioxidants were extracted and identified by spectroscopic analysis; and they were also quantified using the Folin-Denis method and a gallic acid calibration curve, which confirmed the presence of natural antioxidants in the films.
29329275	1	63	theme	apparent	209:216	arg1	density					218:224	apparent density	209:224	apparent density	209:224	The aim of this study is to analyze the properties of a series of polysaccharide composite films, such as apparent density, color, the presence of functional groups, morphology, and thermal stability, as well as the correlation between them and their antimicrobial and optical properties.
29363764	0	0	theme	glycosylation	67:79	arg1	pathway					81:87	the N-linked glycosylation pathway	54:87	the N-linked glycosylation pathway	54:87	Myasthenic syndromes due to defects in COL13A1 and in the N-linked glycosylation pathway.
29363764	7	1	theme	underlying	1405:1414	arg1	mechanisms					1427:1436	the underlying pathogenic mechanisms	1401:1436	the underlying pathogenic mechanisms	1401:1436	Knowledge about the neuromuscular synapse and the different proteins involved in maintaining its structure as well as function enables us to tailor treatments to the underlying pathogenic mechanisms.
29363764	5	2	dep	disorders	773:781	arg1	disorders					795:803	congenital disorders	784:803	congenital disorders of glycosylation	784:820	However, mutations in these genes may also give rise to multisystem disorders (congenital disorders of glycosylation) or muscle disorders where the myasthenic symptoms constitute only one component within a wider phenotypic spectrum.
29363764	4	3	theme	limb-girdle	470:480	arg1	pattern					482:488	a limb-girdle pattern	468:488	a limb-girdle pattern of muscle weakness	468:507	An emerging group of CMS, characterized by a limb-girdle pattern of muscle weakness, is caused by mutations in genes that encode proteins involved in the initial steps of the N-linked glycosylation pathway, which is surprising, since this pathway is found in all mammalian cells.
29363764	1	4	theme	hereditary	136:145	arg1	syndromes					116:124	The congenital myasthenic syndromes	90:124	The congenital myasthenic syndromes (CMS)	90:130	The congenital myasthenic syndromes (CMS) are hereditary disorders of neuromuscular transmission.
29363764	1	4	theme	hereditary	136:145	arg1	disorders					147:155	hereditary disorders	136:155	hereditary disorders of neuromuscular transmission	136:185	The congenital myasthenic syndromes (CMS) are hereditary disorders of neuromuscular transmission.
29363764	6	5	theme	due	960:962	arg1	CMS					956:958	a CMS	954:958	a CMS	954:958	We also report a CMS due to mutations in COL13A1, which encodes an extracellular matrix protein that is concentrated at the neuromuscular junction and highlights a role for these extracellular matrix proteins in maintaining synaptic stability that is independent of the AGRN/MuSK clustering pathway.
29363764	6	5	theme	due	960:962	arg1	protein					1027:1033	an extracellular matrix protein	1003:1033	an extracellular matrix protein that is concentrated at the neuromuscular junction and highlights a role for these extracellular matrix proteins in maintaining synaptic stability that is independent of the AGRN/MuSK clustering pathway	1003:1236	We also report a CMS due to mutations in COL13A1, which encodes an extracellular matrix protein that is concentrated at the neuromuscular junction and highlights a role for these extracellular matrix proteins in maintaining synaptic stability that is independent of the AGRN/MuSK clustering pathway.
29363764	0	6	theme	N-linked	58:65	arg1	pathway					81:87	the N-linked glycosylation pathway	54:87	the N-linked glycosylation pathway	54:87	Myasthenic syndromes due to defects in COL13A1 and in the N-linked glycosylation pathway.
29363764	6	7	from	mutations	967:975	arg1	COL13A1					980:986	COL13A1	980:986	COL13A1	980:986	We also report a CMS due to mutations in COL13A1, which encodes an extracellular matrix protein that is concentrated at the neuromuscular junction and highlights a role for these extracellular matrix proteins in maintaining synaptic stability that is independent of the AGRN/MuSK clustering pathway.
29363764	4	8	theme	weakness	500:507	arg1	pattern					482:488	a limb-girdle pattern	468:488	a limb-girdle pattern of muscle weakness	468:507	An emerging group of CMS, characterized by a limb-girdle pattern of muscle weakness, is caused by mutations in genes that encode proteins involved in the initial steps of the N-linked glycosylation pathway, which is surprising, since this pathway is found in all mammalian cells.
29363764	7	9	theme	pathogenic	1416:1425	arg1	mechanisms					1427:1436	the underlying pathogenic mechanisms	1401:1436	the underlying pathogenic mechanisms	1401:1436	Knowledge about the neuromuscular synapse and the different proteins involved in maintaining its structure as well as function enables us to tailor treatments to the underlying pathogenic mechanisms.
29363764	5	10	theme	phenotypic	918:927	arg1	spectrum					929:936	a wider phenotypic spectrum	910:936	a wider phenotypic spectrum	910:936	However, mutations in these genes may also give rise to multisystem disorders (congenital disorders of glycosylation) or muscle disorders where the myasthenic symptoms constitute only one component within a wider phenotypic spectrum.
29363764	5	11	theme	myasthenic	853:862	arg1	symptoms					864:871	the myasthenic symptoms	849:871	the myasthenic symptoms	849:871	However, mutations in these genes may also give rise to multisystem disorders (congenital disorders of glycosylation) or muscle disorders where the myasthenic symptoms constitute only one component within a wider phenotypic spectrum.
29363764	3	12	theme	sequencing	332:341	arg1	advent					306:311	The advent	302:311	The advent of next-generation sequencing	302:341	The advent of next-generation sequencing has facilitated the discovery of many genes that harbor CMS-associated mutations.
29363764	7	13	theme	different	1289:1297	arg1	proteins					1299:1306	the different proteins	1285:1306	the different proteins involved in maintaining its structure as well as function	1285:1364	Knowledge about the neuromuscular synapse and the different proteins involved in maintaining its structure as well as function enables us to tailor treatments to the underlying pathogenic mechanisms.
29363764	6	14	theme	matrix	1132:1137	arg1	proteins					1139:1146	these extracellular matrix proteins	1112:1146	these extracellular matrix proteins	1112:1146	We also report a CMS due to mutations in COL13A1, which encodes an extracellular matrix protein that is concentrated at the neuromuscular junction and highlights a role for these extracellular matrix proteins in maintaining synaptic stability that is independent of the AGRN/MuSK clustering pathway.
29363764	5	15	theme	muscle	826:831	arg1	disorders					833:841	muscle disorders	826:841	muscle disorders	826:841	However, mutations in these genes may also give rise to multisystem disorders (congenital disorders of glycosylation) or muscle disorders where the myasthenic symptoms constitute only one component within a wider phenotypic spectrum.
29363764	4	16	from	mutations	523:531	arg1	genes					536:540	genes	536:540	genes that encode proteins involved in the initial steps of the N-linked glycosylation pathway, which is surprising, since this pathway is found in all mammalian cells	536:702	An emerging group of CMS, characterized by a limb-girdle pattern of muscle weakness, is caused by mutations in genes that encode proteins involved in the initial steps of the N-linked glycosylation pathway, which is surprising, since this pathway is found in all mammalian cells.
29363764	4	16	from	mutations	523:531	arg1	proteins					554:561	proteins	554:561	proteins involved in the initial steps of the N-linked glycosylation pathway, which is surprising, since this pathway is found in all mammalian cells	554:702	An emerging group of CMS, characterized by a limb-girdle pattern of muscle weakness, is caused by mutations in genes that encode proteins involved in the initial steps of the N-linked glycosylation pathway, which is surprising, since this pathway is found in all mammalian cells.
29363764	3	17	theme	genes	381:385	arg1	discovery					363:371	the discovery	359:371	the discovery of many genes that harbor CMS-associated mutations	359:422	The advent of next-generation sequencing has facilitated the discovery of many genes that harbor CMS-associated mutations.
29363764	1	18	theme	neuromuscular	160:172	arg1	transmission					174:185	neuromuscular transmission	160:185	neuromuscular transmission	160:185	The congenital myasthenic syndromes (CMS) are hereditary disorders of neuromuscular transmission.
29363764	3	19	theme	many	376:379	arg1	genes					381:385	many genes	376:385	many genes that harbor CMS-associated mutations	376:422	The advent of next-generation sequencing has facilitated the discovery of many genes that harbor CMS-associated mutations.
29363764	0	20	from	defects	28:34	arg1	COL13A1					39:45	COL13A1	39:45	COL13A1	39:45	Myasthenic syndromes due to defects in COL13A1 and in the N-linked glycosylation pathway.
29363764	0	20	from	defects	28:34	arg1	pathway					81:87	the N-linked glycosylation pathway	54:87	the N-linked glycosylation pathway	54:87	Myasthenic syndromes due to defects in COL13A1 and in the N-linked glycosylation pathway.
29363764	6	21	theme	clustering	1219:1228	arg1	pathway					1230:1236	the AGRN/MuSK clustering pathway	1205:1236	the AGRN/MuSK clustering pathway	1205:1236	We also report a CMS due to mutations in COL13A1, which encodes an extracellular matrix protein that is concentrated at the neuromuscular junction and highlights a role for these extracellular matrix proteins in maintaining synaptic stability that is independent of the AGRN/MuSK clustering pathway.
29363764	1	22	theme	transmission	174:185	arg1	syndromes					116:124	The congenital myasthenic syndromes	90:124	The congenital myasthenic syndromes (CMS)	90:130	The congenital myasthenic syndromes (CMS) are hereditary disorders of neuromuscular transmission.
29363764	1	22	theme	transmission	174:185	arg1	disorders					147:155	hereditary disorders	136:155	hereditary disorders of neuromuscular transmission	136:185	The congenital myasthenic syndromes (CMS) are hereditary disorders of neuromuscular transmission.
29363764	0	23	theme	Myasthenic	0:9	arg1	syndromes					11:19	Myasthenic syndromes	0:19	Myasthenic syndromes	0:19	Myasthenic syndromes due to defects in COL13A1 and in the N-linked glycosylation pathway.
29363764	6	24	theme	AGRN/MuSK	1209:1217	arg1	pathway					1230:1236	the AGRN/MuSK clustering pathway	1205:1236	the AGRN/MuSK clustering pathway	1205:1236	We also report a CMS due to mutations in COL13A1, which encodes an extracellular matrix protein that is concentrated at the neuromuscular junction and highlights a role for these extracellular matrix proteins in maintaining synaptic stability that is independent of the AGRN/MuSK clustering pathway.
29363764	6	25	theme	extracellular	1006:1018	arg1	CMS					956:958	a CMS	954:958	a CMS	954:958	We also report a CMS due to mutations in COL13A1, which encodes an extracellular matrix protein that is concentrated at the neuromuscular junction and highlights a role for these extracellular matrix proteins in maintaining synaptic stability that is independent of the AGRN/MuSK clustering pathway.
29363764	6	25	theme	extracellular	1006:1018	arg1	protein					1027:1033	an extracellular matrix protein	1003:1033	an extracellular matrix protein that is concentrated at the neuromuscular junction and highlights a role for these extracellular matrix proteins in maintaining synaptic stability that is independent of the AGRN/MuSK clustering pathway	1003:1236	We also report a CMS due to mutations in COL13A1, which encodes an extracellular matrix protein that is concentrated at the neuromuscular junction and highlights a role for these extracellular matrix proteins in maintaining synaptic stability that is independent of the AGRN/MuSK clustering pathway.
29363764	5	26	theme	multisystem	761:771	arg1	disorders					773:781	multisystem disorders	761:781	multisystem disorders (congenital disorders of glycosylation)	761:821	However, mutations in these genes may also give rise to multisystem disorders (congenital disorders of glycosylation) or muscle disorders where the myasthenic symptoms constitute only one component within a wider phenotypic spectrum.
29363764	2	27	theme	improved	282:289	arg1	diagnosis					291:299	improved diagnosis	282:299	improved diagnosis	282:299	The number of cases recognized, at around 1:100,000 in the United Kingdom, is increasing with improved diagnosis.
29363764	2	28	from	1:100,000	230:238	arg1	Kingdom					254:260	the United Kingdom	243:260	the United Kingdom	243:260	The number of cases recognized, at around 1:100,000 in the United Kingdom, is increasing with improved diagnosis.
29363764	4	29	theme	glycosylation	609:621	arg1	pathway					623:629	the N-linked glycosylation pathway	596:629	the N-linked glycosylation pathway	596:629	An emerging group of CMS, characterized by a limb-girdle pattern of muscle weakness, is caused by mutations in genes that encode proteins involved in the initial steps of the N-linked glycosylation pathway, which is surprising, since this pathway is found in all mammalian cells.
29363764	4	29	theme	glycosylation	609:621	arg1	surprising					641:650	surprising	641:650	surprising	641:650	An emerging group of CMS, characterized by a limb-girdle pattern of muscle weakness, is caused by mutations in genes that encode proteins involved in the initial steps of the N-linked glycosylation pathway, which is surprising, since this pathway is found in all mammalian cells.
29363764	4	30	theme	CMS	446:448	arg1	CMS					446:448	CMS	446:448	CMS	446:448	An emerging group of CMS, characterized by a limb-girdle pattern of muscle weakness, is caused by mutations in genes that encode proteins involved in the initial steps of the N-linked glycosylation pathway, which is surprising, since this pathway is found in all mammalian cells.
29363764	4	30	theme	CMS	446:448	arg1	group					437:441	An emerging group	425:441	An emerging group	425:441	An emerging group of CMS, characterized by a limb-girdle pattern of muscle weakness, is caused by mutations in genes that encode proteins involved in the initial steps of the N-linked glycosylation pathway, which is surprising, since this pathway is found in all mammalian cells.
29363764	1	31	theme	congenital	94:103	arg1	CMS					127:129	CMS	127:129	CMS	127:129	The congenital myasthenic syndromes (CMS) are hereditary disorders of neuromuscular transmission.
29363764	1	31	theme	congenital	94:103	arg1	syndromes					116:124	The congenital myasthenic syndromes	90:124	The congenital myasthenic syndromes (CMS)	90:130	The congenital myasthenic syndromes (CMS) are hereditary disorders of neuromuscular transmission.
29363764	1	31	theme	congenital	94:103	arg1	disorders					147:155	hereditary disorders	136:155	hereditary disorders of neuromuscular transmission	136:185	The congenital myasthenic syndromes (CMS) are hereditary disorders of neuromuscular transmission.
29363764	4	32	theme	N-linked	600:607	arg1	pathway					623:629	the N-linked glycosylation pathway	596:629	the N-linked glycosylation pathway	596:629	An emerging group of CMS, characterized by a limb-girdle pattern of muscle weakness, is caused by mutations in genes that encode proteins involved in the initial steps of the N-linked glycosylation pathway, which is surprising, since this pathway is found in all mammalian cells.
29363764	4	32	theme	N-linked	600:607	arg1	surprising					641:650	surprising	641:650	surprising	641:650	An emerging group of CMS, characterized by a limb-girdle pattern of muscle weakness, is caused by mutations in genes that encode proteins involved in the initial steps of the N-linked glycosylation pathway, which is surprising, since this pathway is found in all mammalian cells.
29363764	3	33	theme	CMS-associated	399:412	arg1	mutations					414:422	CMS-associated mutations	399:422	CMS-associated mutations	399:422	The advent of next-generation sequencing has facilitated the discovery of many genes that harbor CMS-associated mutations.
29363764	6	34	theme	matrix	1020:1025	arg1	CMS					956:958	a CMS	954:958	a CMS	954:958	We also report a CMS due to mutations in COL13A1, which encodes an extracellular matrix protein that is concentrated at the neuromuscular junction and highlights a role for these extracellular matrix proteins in maintaining synaptic stability that is independent of the AGRN/MuSK clustering pathway.
29363764	6	34	theme	matrix	1020:1025	arg1	protein					1027:1033	an extracellular matrix protein	1003:1033	an extracellular matrix protein that is concentrated at the neuromuscular junction and highlights a role for these extracellular matrix proteins in maintaining synaptic stability that is independent of the AGRN/MuSK clustering pathway	1003:1236	We also report a CMS due to mutations in COL13A1, which encodes an extracellular matrix protein that is concentrated at the neuromuscular junction and highlights a role for these extracellular matrix proteins in maintaining synaptic stability that is independent of the AGRN/MuSK clustering pathway.
29363764	5	35	theme	glycosylation	808:820	arg1	disorders					795:803	congenital disorders	784:803	congenital disorders of glycosylation	784:820	However, mutations in these genes may also give rise to multisystem disorders (congenital disorders of glycosylation) or muscle disorders where the myasthenic symptoms constitute only one component within a wider phenotypic spectrum.
29363764	4	36	theme	pathway	623:629	arg1	steps					587:591	the initial steps	575:591	the initial steps of the N-linked glycosylation pathway, which is surprising, since this pathway is found in all mammalian cells	575:702	An emerging group of CMS, characterized by a limb-girdle pattern of muscle weakness, is caused by mutations in genes that encode proteins involved in the initial steps of the N-linked glycosylation pathway, which is surprising, since this pathway is found in all mammalian cells.
29363764	0	37	link	N-linked	58:65	arg1	pathway					81:87	the N-linked glycosylation pathway	54:87	the N-linked glycosylation pathway	54:87	Myasthenic syndromes due to defects in COL13A1 and in the N-linked glycosylation pathway.
29363764	1	38	theme	myasthenic	105:114	arg1	CMS					127:129	CMS	127:129	CMS	127:129	The congenital myasthenic syndromes (CMS) are hereditary disorders of neuromuscular transmission.
29363764	1	38	theme	myasthenic	105:114	arg1	syndromes					116:124	The congenital myasthenic syndromes	90:124	The congenital myasthenic syndromes (CMS)	90:130	The congenital myasthenic syndromes (CMS) are hereditary disorders of neuromuscular transmission.
29363764	1	38	theme	myasthenic	105:114	arg1	disorders					147:155	hereditary disorders	136:155	hereditary disorders of neuromuscular transmission	136:185	The congenital myasthenic syndromes (CMS) are hereditary disorders of neuromuscular transmission.
29363764	4	39	theme	muscle	493:498	arg1	weakness					500:507	muscle weakness	493:507	muscle weakness	493:507	An emerging group of CMS, characterized by a limb-girdle pattern of muscle weakness, is caused by mutations in genes that encode proteins involved in the initial steps of the N-linked glycosylation pathway, which is surprising, since this pathway is found in all mammalian cells.
29363764	2	40	theme	cases	202:206	arg1	number					192:197	The number	188:197	The number of cases recognized	188:217	The number of cases recognized, at around 1:100,000 in the United Kingdom, is increasing with improved diagnosis.
29363764	6	41	theme	pathway	1230:1236	arg1	independent					1190:1200	independent	1190:1200	independent	1190:1200	We also report a CMS due to mutations in COL13A1, which encodes an extracellular matrix protein that is concentrated at the neuromuscular junction and highlights a role for these extracellular matrix proteins in maintaining synaptic stability that is independent of the AGRN/MuSK clustering pathway.
29363764	6	41	theme	pathway	1230:1236	arg1	role					1103:1106	a role	1101:1106	a role for these extracellular matrix proteins in maintaining synaptic stability that is independent of the AGRN/MuSK clustering pathway	1101:1236	We also report a CMS due to mutations in COL13A1, which encodes an extracellular matrix protein that is concentrated at the neuromuscular junction and highlights a role for these extracellular matrix proteins in maintaining synaptic stability that is independent of the AGRN/MuSK clustering pathway.
29363764	7	42	theme	neuromuscular	1259:1271	arg1	synapse					1273:1279	the neuromuscular synapse	1255:1279	the neuromuscular synapse	1255:1279	Knowledge about the neuromuscular synapse and the different proteins involved in maintaining its structure as well as function enables us to tailor treatments to the underlying pathogenic mechanisms.
29363764	2	43	theme	United	247:252	arg1	Kingdom					254:260	the United Kingdom	243:260	the United Kingdom	243:260	The number of cases recognized, at around 1:100,000 in the United Kingdom, is increasing with improved diagnosis.
29363764	4	44	theme	mammalian	688:696	arg1	cells					698:702	all mammalian cells	684:702	all mammalian cells	684:702	An emerging group of CMS, characterized by a limb-girdle pattern of muscle weakness, is caused by mutations in genes that encode proteins involved in the initial steps of the N-linked glycosylation pathway, which is surprising, since this pathway is found in all mammalian cells.
29363764	5	45	theme	wider	912:916	arg1	spectrum					929:936	a wider phenotypic spectrum	910:936	a wider phenotypic spectrum	910:936	However, mutations in these genes may also give rise to multisystem disorders (congenital disorders of glycosylation) or muscle disorders where the myasthenic symptoms constitute only one component within a wider phenotypic spectrum.
29363764	6	46	theme	extracellular	1118:1130	arg1	proteins					1139:1146	these extracellular matrix proteins	1112:1146	these extracellular matrix proteins	1112:1146	We also report a CMS due to mutations in COL13A1, which encodes an extracellular matrix protein that is concentrated at the neuromuscular junction and highlights a role for these extracellular matrix proteins in maintaining synaptic stability that is independent of the AGRN/MuSK clustering pathway.
29363764	5	47	from	mutations	714:722	arg1	genes					733:737	these genes	727:737	these genes	727:737	However, mutations in these genes may also give rise to multisystem disorders (congenital disorders of glycosylation) or muscle disorders where the myasthenic symptoms constitute only one component within a wider phenotypic spectrum.
29363764	6	48	theme	neuromuscular	1063:1075	arg1	junction					1077:1084	the neuromuscular junction	1059:1084	the neuromuscular junction	1059:1084	We also report a CMS due to mutations in COL13A1, which encodes an extracellular matrix protein that is concentrated at the neuromuscular junction and highlights a role for these extracellular matrix proteins in maintaining synaptic stability that is independent of the AGRN/MuSK clustering pathway.
29363764	4	49	theme	initial	579:585	arg1	steps					587:591	the initial steps	575:591	the initial steps of the N-linked glycosylation pathway, which is surprising, since this pathway is found in all mammalian cells	575:702	An emerging group of CMS, characterized by a limb-girdle pattern of muscle weakness, is caused by mutations in genes that encode proteins involved in the initial steps of the N-linked glycosylation pathway, which is surprising, since this pathway is found in all mammalian cells.
29363764	4	50	theme	emerging	428:435	arg1	CMS					446:448	CMS	446:448	CMS	446:448	An emerging group of CMS, characterized by a limb-girdle pattern of muscle weakness, is caused by mutations in genes that encode proteins involved in the initial steps of the N-linked glycosylation pathway, which is surprising, since this pathway is found in all mammalian cells.
29363764	4	50	theme	emerging	428:435	arg1	group					437:441	An emerging group	425:441	An emerging group	425:441	An emerging group of CMS, characterized by a limb-girdle pattern of muscle weakness, is caused by mutations in genes that encode proteins involved in the initial steps of the N-linked glycosylation pathway, which is surprising, since this pathway is found in all mammalian cells.
29363764	6	51	theme	synaptic	1163:1170	arg1	stability					1172:1180	synaptic stability	1163:1180	synaptic stability	1163:1180	We also report a CMS due to mutations in COL13A1, which encodes an extracellular matrix protein that is concentrated at the neuromuscular junction and highlights a role for these extracellular matrix proteins in maintaining synaptic stability that is independent of the AGRN/MuSK clustering pathway.
29363764	5	52	theme	congenital	784:793	arg1	disorders					795:803	congenital disorders	784:803	congenital disorders of glycosylation	784:820	However, mutations in these genes may also give rise to multisystem disorders (congenital disorders of glycosylation) or muscle disorders where the myasthenic symptoms constitute only one component within a wider phenotypic spectrum.
29363764	3	53	theme	next-generation	316:330	arg1	sequencing					332:341	next-generation sequencing	316:341	next-generation sequencing	316:341	The advent of next-generation sequencing has facilitated the discovery of many genes that harbor CMS-associated mutations.
29363764	4	54	located	found	675:679	arg1	cells					698:702	all mammalian cells	684:702	all mammalian cells	684:702	An emerging group of CMS, characterized by a limb-girdle pattern of muscle weakness, is caused by mutations in genes that encode proteins involved in the initial steps of the N-linked glycosylation pathway, which is surprising, since this pathway is found in all mammalian cells.
29363764	4	54	located	found	675:679	arg2	pathway					664:670	this pathway	659:670	this pathway	659:670	An emerging group of CMS, characterized by a limb-girdle pattern of muscle weakness, is caused by mutations in genes that encode proteins involved in the initial steps of the N-linked glycosylation pathway, which is surprising, since this pathway is found in all mammalian cells.
29363764	4	55	link	N-linked	600:607	arg1	pathway					623:629	the N-linked glycosylation pathway	596:629	the N-linked glycosylation pathway	596:629	An emerging group of CMS, characterized by a limb-girdle pattern of muscle weakness, is caused by mutations in genes that encode proteins involved in the initial steps of the N-linked glycosylation pathway, which is surprising, since this pathway is found in all mammalian cells.
29363764	4	55	link	N-linked	600:607	arg1	surprising					641:650	surprising	641:650	surprising	641:650	An emerging group of CMS, characterized by a limb-girdle pattern of muscle weakness, is caused by mutations in genes that encode proteins involved in the initial steps of the N-linked glycosylation pathway, which is surprising, since this pathway is found in all mammalian cells.
31887897	8	0	theme	gastrointestinal	1215:1230	arg1	tract					1232:1236	the in vitro gastrointestinal tract	1202:1236	the in vitro gastrointestinal tract simulation	1202:1247	In vitro digestion showed that PSF was minimally digested with the in vitro gastrointestinal tract simulation, but PSF with UV/H2O2 treatment could be digested in the low acid environment in the simulated gastric juice, but was minimally digested with the simulated intestinal juice.
31887897	3	1	with	2 h	485:487	arg1	H2O2					508:511	UV/150 mmol/L H2O2	494:511	UV/150 mmol/L H2O2	494:511	Results indicated that the molecular weight of PSF was reduced from ∼289 to ∼12.6 kDa within 2 h with UV/150 mmol/L H2O2, and the depolymerization effect of UV/H2O2 was significantly higher than that of UV or H2O2 alone.
31887897	1	2	from	fusiforme	194:202	arg1	polysaccharides					163:177	the polysaccharides	159:177	the polysaccharides from Sargassum fusiforme (PSF), a brown algae,	159:224	The depolymerization effect of UV/H2O2 on the polysaccharides from Sargassum fusiforme (PSF), a brown algae, were studied.
31887897	4	3	theme	recovery	659:666	arg1	%					695:695	93.54 %	689:695	93.54 %	689:695	In addition, the UV/H2O2 treatment had a high recovery rate of total sugar (93.54 %) and clearance rate of protein (76.34 %).
31887897	4	3	theme	recovery	659:666	arg1	rate					668:671	a high recovery rate	652:671	a high recovery rate of total sugar	652:686	In addition, the UV/H2O2 treatment had a high recovery rate of total sugar (93.54 %) and clearance rate of protein (76.34 %).
31887897	5	4	theme	mole	873:876	arg1	percentage					878:887	the mole percentage	869:887	the mole percentage of fucose	869:897	The monosaccharide composition showed that UV/H2O2 treatment could increase the mole percentage of mannose (37.44 %) and decrease the mole percentage of fucose (14.88 %).
31887897	4	5	contain	had	648:650	arg2	%					695:695	93.54 %	689:695	93.54 %	689:695	In addition, the UV/H2O2 treatment had a high recovery rate of total sugar (93.54 %) and clearance rate of protein (76.34 %).
31887897	4	5	contain	had	648:650	arg2	rate					712:715	clearance rate	702:715	clearance rate of protein (76.34 %)	702:736	In addition, the UV/H2O2 treatment had a high recovery rate of total sugar (93.54 %) and clearance rate of protein (76.34 %).
31887897	4	5	contain	had	648:650	arg2	%					735:735	76.34 %	729:735	76.34 %	729:735	In addition, the UV/H2O2 treatment had a high recovery rate of total sugar (93.54 %) and clearance rate of protein (76.34 %).
31887897	4	5	contain	had	648:650	arg2	rate					668:671	a high recovery rate	652:671	a high recovery rate of total sugar	652:686	In addition, the UV/H2O2 treatment had a high recovery rate of total sugar (93.54 %) and clearance rate of protein (76.34 %).
31887897	4	5	contain	had	648:650	arg1	treatment					638:646	the UV/H2O2 treatment	626:646	the UV/H2O2 treatment	626:646	In addition, the UV/H2O2 treatment had a high recovery rate of total sugar (93.54 %) and clearance rate of protein (76.34 %).
31887897	2	6	theme	weight	334:339	arg1	changes					341:347	molecular weight changes	324:347	molecular weight changes during in vitro digestion	324:373	The structural changes of PSF before and after UV/H2O2 treatment were analyzed, and molecular weight changes during in vitro digestion were determined.
31887897	0	7	from	Degradation	0:10	arg1	Sargassum					36:44	Sargassum	36:44	Sargassum	36:44	Degradation of polysaccharides from Sargassum fusiforme using UV/H2O2 and its effects on structural characteristics.
31887897	4	8	theme	clearance	702:710	arg1	%					735:735	76.34 %	729:735	76.34 %	729:735	In addition, the UV/H2O2 treatment had a high recovery rate of total sugar (93.54 %) and clearance rate of protein (76.34 %).
31887897	4	8	theme	clearance	702:710	arg1	rate					712:715	clearance rate	702:715	clearance rate of protein (76.34 %)	702:736	In addition, the UV/H2O2 treatment had a high recovery rate of total sugar (93.54 %) and clearance rate of protein (76.34 %).
31887897	3	9	dep	∼12.6 kDa	468:476	arg1	to					465:466	to	465:466	to	465:466	Results indicated that the molecular weight of PSF was reduced from ∼289 to ∼12.6 kDa within 2 h with UV/150 mmol/L H2O2, and the depolymerization effect of UV/H2O2 was significantly higher than that of UV or H2O2 alone.
31887897	0	10	theme	structural	89:98	arg1	characteristics					100:114	structural characteristics	89:114	structural characteristics	89:114	Degradation of polysaccharides from Sargassum fusiforme using UV/H2O2 and its effects on structural characteristics.
31887897	0	11	from	UV/H2O2	62:68	arg1	characteristics					100:114	structural characteristics	89:114	structural characteristics	89:114	Degradation of polysaccharides from Sargassum fusiforme using UV/H2O2 and its effects on structural characteristics.
31887897	9	12	dep	preparation	1455:1465	arg1	the					1451:1453	the	1451:1453	the	1451:1453	This studied suggested that the preparation and application of functional PSF with low molecular weight might be beneficial.
31887897	3	13	theme	depolymerization	522:537	arg1	higher					575:580	higher	575:580	higher	575:580	Results indicated that the molecular weight of PSF was reduced from ∼289 to ∼12.6 kDa within 2 h with UV/150 mmol/L H2O2, and the depolymerization effect of UV/H2O2 was significantly higher than that of UV or H2O2 alone.
31887897	3	13	theme	depolymerization	522:537	arg1	effect					539:544	the depolymerization effect	518:544	the depolymerization effect of UV/H2O2	518:555	Results indicated that the molecular weight of PSF was reduced from ∼289 to ∼12.6 kDa within 2 h with UV/150 mmol/L H2O2, and the depolymerization effect of UV/H2O2 was significantly higher than that of UV or H2O2 alone.
31887897	4	14	theme	total	676:680	arg1	sugar					682:686	total sugar	676:686	total sugar	676:686	In addition, the UV/H2O2 treatment had a high recovery rate of total sugar (93.54 %) and clearance rate of protein (76.34 %).
31887897	1	15	theme	depolymerization	121:136	arg1	effect					138:143	The depolymerization effect	117:143	The depolymerization effect of UV/H2O2 on the polysaccharides from Sargassum fusiforme (PSF), a brown algae,	117:224	The depolymerization effect of UV/H2O2 on the polysaccharides from Sargassum fusiforme (PSF), a brown algae, were studied.
31887897	2	16	theme	PSF	266:268	arg1	changes					255:261	The structural changes	240:261	The structural changes of PSF before and after UV/H2O2 treatment	240:303	The structural changes of PSF before and after UV/H2O2 treatment were analyzed, and molecular weight changes during in vitro digestion were determined.
31887897	1	17	theme	Sargassum	184:192	arg1	algae					219:223	a brown algae	211:223	a brown algae	211:223	The depolymerization effect of UV/H2O2 on the polysaccharides from Sargassum fusiforme (PSF), a brown algae, were studied.
31887897	1	17	theme	Sargassum	184:192	arg1	PSF					205:207	PSF	205:207	PSF	205:207	The depolymerization effect of UV/H2O2 on the polysaccharides from Sargassum fusiforme (PSF), a brown algae, were studied.
31887897	1	17	theme	Sargassum	184:192	arg1	fusiforme					194:202	Sargassum fusiforme	184:202	Sargassum fusiforme (PSF)	184:208	The depolymerization effect of UV/H2O2 on the polysaccharides from Sargassum fusiforme (PSF), a brown algae, were studied.
31887897	5	18	theme	mannose	838:844	arg1	percentage					824:833	the mole percentage	815:833	the mole percentage of mannose (37.44 %)	815:854	The monosaccharide composition showed that UV/H2O2 treatment could increase the mole percentage of mannose (37.44 %) and decrease the mole percentage of fucose (14.88 %).
31887897	4	19	theme	protein	720:726	arg1	%					695:695	93.54 %	689:695	93.54 %	689:695	In addition, the UV/H2O2 treatment had a high recovery rate of total sugar (93.54 %) and clearance rate of protein (76.34 %).
31887897	4	19	theme	protein	720:726	arg1	%					735:735	76.34 %	729:735	76.34 %	729:735	In addition, the UV/H2O2 treatment had a high recovery rate of total sugar (93.54 %) and clearance rate of protein (76.34 %).
31887897	4	19	theme	protein	720:726	arg1	rate					712:715	clearance rate	702:715	clearance rate of protein (76.34 %)	702:736	In addition, the UV/H2O2 treatment had a high recovery rate of total sugar (93.54 %) and clearance rate of protein (76.34 %).
31887897	4	19	theme	protein	720:726	arg1	rate					668:671	a high recovery rate	652:671	a high recovery rate of total sugar	652:686	In addition, the UV/H2O2 treatment had a high recovery rate of total sugar (93.54 %) and clearance rate of protein (76.34 %).
31887897	9	20	with	application	1471:1481	arg1	weight					1520:1525	low molecular weight	1506:1525	low molecular weight	1506:1525	This studied suggested that the preparation and application of functional PSF with low molecular weight might be beneficial.
31887897	0	21	from	Sargassum	36:44	arg1	polysaccharides					15:29	polysaccharides	15:29	polysaccharides from Sargassum	15:44	Degradation of polysaccharides from Sargassum fusiforme using UV/H2O2 and its effects on structural characteristics.
31887897	0	21	from	Sargassum	36:44	arg1	Degradation					0:10	Degradation	0:10	Degradation of polysaccharides from Sargassum	0:44	Degradation of polysaccharides from Sargassum fusiforme using UV/H2O2 and its effects on structural characteristics.
31887897	3	22	theme	UV/H2O2	549:555	arg1	higher					575:580	higher	575:580	higher	575:580	Results indicated that the molecular weight of PSF was reduced from ∼289 to ∼12.6 kDa within 2 h with UV/150 mmol/L H2O2, and the depolymerization effect of UV/H2O2 was significantly higher than that of UV or H2O2 alone.
31887897	3	22	theme	UV/H2O2	549:555	arg1	effect					539:544	the depolymerization effect	518:544	the depolymerization effect of UV/H2O2	518:555	Results indicated that the molecular weight of PSF was reduced from ∼289 to ∼12.6 kDa within 2 h with UV/150 mmol/L H2O2, and the depolymerization effect of UV/H2O2 was significantly higher than that of UV or H2O2 alone.
31887897	8	23	theme	intestinal	1405:1414	arg1	juice					1416:1420	the simulated intestinal juice	1391:1420	the simulated intestinal juice	1391:1420	In vitro digestion showed that PSF was minimally digested with the in vitro gastrointestinal tract simulation, but PSF with UV/H2O2 treatment could be digested in the low acid environment in the simulated gastric juice, but was minimally digested with the simulated intestinal juice.
31887897	9	24	theme	functional	1486:1495	arg1	PSF					1497:1499	functional PSF	1486:1499	functional PSF	1486:1499	This studied suggested that the preparation and application of functional PSF with low molecular weight might be beneficial.
31887897	8	25	theme	simulated	1395:1403	arg1	juice					1416:1420	the simulated intestinal juice	1391:1420	the simulated intestinal juice	1391:1420	In vitro digestion showed that PSF was minimally digested with the in vitro gastrointestinal tract simulation, but PSF with UV/H2O2 treatment could be digested in the low acid environment in the simulated gastric juice, but was minimally digested with the simulated intestinal juice.
31887897	8	26	theme	In	1139:1140	arg1	digestion					1148:1156	In vitro digestion	1139:1156	In vitro digestion	1139:1156	In vitro digestion showed that PSF was minimally digested with the in vitro gastrointestinal tract simulation, but PSF with UV/H2O2 treatment could be digested in the low acid environment in the simulated gastric juice, but was minimally digested with the simulated intestinal juice.
31887897	8	27	theme	low	1306:1308	arg1	environment					1315:1325	the low acid environment	1302:1325	the low acid environment in the simulated gastric juice	1302:1356	In vitro digestion showed that PSF was minimally digested with the in vitro gastrointestinal tract simulation, but PSF with UV/H2O2 treatment could be digested in the low acid environment in the simulated gastric juice, but was minimally digested with the simulated intestinal juice.
31887897	3	28	theme	molecular	419:427	arg1	weight					429:434	the molecular weight	415:434	the molecular weight of PSF	415:441	Results indicated that the molecular weight of PSF was reduced from ∼289 to ∼12.6 kDa within 2 h with UV/150 mmol/L H2O2, and the depolymerization effect of UV/H2O2 was significantly higher than that of UV or H2O2 alone.
31887897	9	29	with	preparation	1455:1465	arg1	weight					1520:1525	low molecular weight	1506:1525	low molecular weight	1506:1525	This studied suggested that the preparation and application of functional PSF with low molecular weight might be beneficial.
31887897	8	30	theme	gastric	1344:1350	arg1	juice					1352:1356	the simulated gastric juice	1330:1356	the simulated gastric juice	1330:1356	In vitro digestion showed that PSF was minimally digested with the in vitro gastrointestinal tract simulation, but PSF with UV/H2O2 treatment could be digested in the low acid environment in the simulated gastric juice, but was minimally digested with the simulated intestinal juice.
31887897	1	31	from	effect	138:143	arg1	polysaccharides					163:177	the polysaccharides	159:177	the polysaccharides from Sargassum fusiforme (PSF), a brown algae,	159:224	The depolymerization effect of UV/H2O2 on the polysaccharides from Sargassum fusiforme (PSF), a brown algae, were studied.
31887897	2	32	theme	UV/H2O2	287:293	arg1	treatment					295:303	UV/H2O2 treatment	287:303	UV/H2O2 treatment	287:303	The structural changes of PSF before and after UV/H2O2 treatment were analyzed, and molecular weight changes during in vitro digestion were determined.
31887897	8	33	from	environment	1315:1325	arg1	juice					1352:1356	the simulated gastric juice	1330:1356	the simulated gastric juice	1330:1356	In vitro digestion showed that PSF was minimally digested with the in vitro gastrointestinal tract simulation, but PSF with UV/H2O2 treatment could be digested in the low acid environment in the simulated gastric juice, but was minimally digested with the simulated intestinal juice.
31887897	0	34	theme	polysaccharides	15:29	arg1	Degradation					0:10	Degradation	0:10	Degradation of polysaccharides from Sargassum	0:44	Degradation of polysaccharides from Sargassum fusiforme using UV/H2O2 and its effects on structural characteristics.
31887897	2	35	dep	in	356:357	arg1	vitro					359:363	vitro	359:363	vitro	359:363	The structural changes of PSF before and after UV/H2O2 treatment were analyzed, and molecular weight changes during in vitro digestion were determined.
31887897	8	36	theme	simulated	1334:1342	arg1	juice					1352:1356	the simulated gastric juice	1330:1356	the simulated gastric juice	1330:1356	In vitro digestion showed that PSF was minimally digested with the in vitro gastrointestinal tract simulation, but PSF with UV/H2O2 treatment could be digested in the low acid environment in the simulated gastric juice, but was minimally digested with the simulated intestinal juice.
31887897	4	37	theme	high	654:657	arg1	%					695:695	93.54 %	689:695	93.54 %	689:695	In addition, the UV/H2O2 treatment had a high recovery rate of total sugar (93.54 %) and clearance rate of protein (76.34 %).
31887897	4	37	theme	high	654:657	arg1	rate					668:671	a high recovery rate	652:671	a high recovery rate of total sugar	652:686	In addition, the UV/H2O2 treatment had a high recovery rate of total sugar (93.54 %) and clearance rate of protein (76.34 %).
31887897	8	38	theme	UV/H2O2	1263:1269	arg1	treatment					1271:1279	UV/H2O2 treatment	1263:1279	UV/H2O2 treatment	1263:1279	In vitro digestion showed that PSF was minimally digested with the in vitro gastrointestinal tract simulation, but PSF with UV/H2O2 treatment could be digested in the low acid environment in the simulated gastric juice, but was minimally digested with the simulated intestinal juice.
31887897	3	39	theme	UV/150 mmol/L	494:506	arg1	H2O2					508:511	UV/150 mmol/L H2O2	494:511	UV/150 mmol/L H2O2	494:511	Results indicated that the molecular weight of PSF was reduced from ∼289 to ∼12.6 kDa within 2 h with UV/150 mmol/L H2O2, and the depolymerization effect of UV/H2O2 was significantly higher than that of UV or H2O2 alone.
31887897	0	40	from	effects	78:84	arg1	characteristics					100:114	structural characteristics	89:114	structural characteristics	89:114	Degradation of polysaccharides from Sargassum fusiforme using UV/H2O2 and its effects on structural characteristics.
31887897	5	41	dep	increase	806:813	arg1	%					906:906	14.88 %	900:906	14.88 %	900:906	The monosaccharide composition showed that UV/H2O2 treatment could increase the mole percentage of mannose (37.44 %) and decrease the mole percentage of fucose (14.88 %).
31887897	4	42	theme	UV/H2O2	630:636	arg1	treatment					638:646	the UV/H2O2 treatment	626:646	the UV/H2O2 treatment	626:646	In addition, the UV/H2O2 treatment had a high recovery rate of total sugar (93.54 %) and clearance rate of protein (76.34 %).
31887897	3	43	theme	PSF	439:441	arg1	weight					429:434	the molecular weight	415:434	the molecular weight of PSF	415:441	Results indicated that the molecular weight of PSF was reduced from ∼289 to ∼12.6 kDa within 2 h with UV/150 mmol/L H2O2, and the depolymerization effect of UV/H2O2 was significantly higher than that of UV or H2O2 alone.
31887897	6	44	theme	force	972:976	arg1	AFM					990:992	AFM	990:992	AFM	990:992	The helix-coil transition, X-ray diffraction (XRD) and atomic force microscopy (AFM) imaging showed that the UV/H2O2 treatment depolymerized PSF.
31887897	6	44	theme	force	972:976	arg1	microscopy					978:987	atomic force microscopy	965:987	atomic force microscopy (AFM)	965:993	The helix-coil transition, X-ray diffraction (XRD) and atomic force microscopy (AFM) imaging showed that the UV/H2O2 treatment depolymerized PSF.
31887897	6	44	theme	force	972:976	arg1	transition					925:934	The helix-coil transition	910:934	The helix-coil transition	910:934	The helix-coil transition, X-ray diffraction (XRD) and atomic force microscopy (AFM) imaging showed that the UV/H2O2 treatment depolymerized PSF.
31887897	8	45	theme	in	1206:1207	arg1	tract					1232:1236	the in vitro gastrointestinal tract	1202:1236	the in vitro gastrointestinal tract simulation	1202:1247	In vitro digestion showed that PSF was minimally digested with the in vitro gastrointestinal tract simulation, but PSF with UV/H2O2 treatment could be digested in the low acid environment in the simulated gastric juice, but was minimally digested with the simulated intestinal juice.
31887897	7	46	with	PSF	1091:1093	arg1	treatment					1108:1116	UV/H2O2 treatment	1100:1116	UV/H2O2 treatment	1100:1116	Rheological studies indicated that PSF with UV/H2O2 treatment had lower viscosity.
31887897	1	47	theme	brown	213:217	arg1	algae					219:223	a brown algae	211:223	a brown algae	211:223	The depolymerization effect of UV/H2O2 on the polysaccharides from Sargassum fusiforme (PSF), a brown algae, were studied.
31887897	1	47	theme	brown	213:217	arg1	fusiforme					194:202	Sargassum fusiforme	184:202	Sargassum fusiforme (PSF)	184:208	The depolymerization effect of UV/H2O2 on the polysaccharides from Sargassum fusiforme (PSF), a brown algae, were studied.
31887897	5	48	theme	mole	819:822	arg1	percentage					824:833	the mole percentage	815:833	the mole percentage of mannose (37.44 %)	815:854	The monosaccharide composition showed that UV/H2O2 treatment could increase the mole percentage of mannose (37.44 %) and decrease the mole percentage of fucose (14.88 %).
31887897	6	49	theme	X-ray	937:941	arg1	XRD					956:958	XRD	956:958	XRD	956:958	The helix-coil transition, X-ray diffraction (XRD) and atomic force microscopy (AFM) imaging showed that the UV/H2O2 treatment depolymerized PSF.
31887897	6	49	theme	X-ray	937:941	arg1	transition					925:934	The helix-coil transition	910:934	The helix-coil transition	910:934	The helix-coil transition, X-ray diffraction (XRD) and atomic force microscopy (AFM) imaging showed that the UV/H2O2 treatment depolymerized PSF.
31887897	6	49	theme	X-ray	937:941	arg1	diffraction					943:953	X-ray diffraction	937:953	X-ray diffraction (XRD)	937:959	The helix-coil transition, X-ray diffraction (XRD) and atomic force microscopy (AFM) imaging showed that the UV/H2O2 treatment depolymerized PSF.
31887897	5	50	theme	fucose	892:897	arg1	percentage					878:887	the mole percentage	869:887	the mole percentage of fucose	869:897	The monosaccharide composition showed that UV/H2O2 treatment could increase the mole percentage of mannose (37.44 %) and decrease the mole percentage of fucose (14.88 %).
31887897	2	51	theme	molecular	324:332	arg1	changes					341:347	molecular weight changes	324:347	molecular weight changes during in vitro digestion	324:373	The structural changes of PSF before and after UV/H2O2 treatment were analyzed, and molecular weight changes during in vitro digestion were determined.
31887897	8	52	dep	in	1206:1207	arg1	vitro					1209:1213	vitro	1209:1213	vitro	1209:1213	In vitro digestion showed that PSF was minimally digested with the in vitro gastrointestinal tract simulation, but PSF with UV/H2O2 treatment could be digested in the low acid environment in the simulated gastric juice, but was minimally digested with the simulated intestinal juice.
31887897	9	53	theme	molecular	1510:1518	arg1	weight					1520:1525	low molecular weight	1506:1525	low molecular weight	1506:1525	This studied suggested that the preparation and application of functional PSF with low molecular weight might be beneficial.
31887897	8	54	theme	acid	1310:1313	arg1	environment					1315:1325	the low acid environment	1302:1325	the low acid environment in the simulated gastric juice	1302:1356	In vitro digestion showed that PSF was minimally digested with the in vitro gastrointestinal tract simulation, but PSF with UV/H2O2 treatment could be digested in the low acid environment in the simulated gastric juice, but was minimally digested with the simulated intestinal juice.
31887897	9	55	theme	PSF	1497:1499	arg1	application					1471:1481	application	1471:1481	application	1471:1481	This studied suggested that the preparation and application of functional PSF with low molecular weight might be beneficial.
31887897	9	55	theme	PSF	1497:1499	arg1	preparation					1455:1465	preparation	1455:1465	preparation	1455:1465	This studied suggested that the preparation and application of functional PSF with low molecular weight might be beneficial.
31887897	4	56	theme	sugar	682:686	arg1	%					695:695	93.54 %	689:695	93.54 %	689:695	In addition, the UV/H2O2 treatment had a high recovery rate of total sugar (93.54 %) and clearance rate of protein (76.34 %).
31887897	4	56	theme	sugar	682:686	arg1	%					735:735	76.34 %	729:735	76.34 %	729:735	In addition, the UV/H2O2 treatment had a high recovery rate of total sugar (93.54 %) and clearance rate of protein (76.34 %).
31887897	4	56	theme	sugar	682:686	arg1	rate					712:715	clearance rate	702:715	clearance rate of protein (76.34 %)	702:736	In addition, the UV/H2O2 treatment had a high recovery rate of total sugar (93.54 %) and clearance rate of protein (76.34 %).
31887897	4	56	theme	sugar	682:686	arg1	rate					668:671	a high recovery rate	652:671	a high recovery rate of total sugar	652:686	In addition, the UV/H2O2 treatment had a high recovery rate of total sugar (93.54 %) and clearance rate of protein (76.34 %).
31887897	8	57	dep	In	1139:1140	arg1	vitro					1142:1146	vitro	1142:1146	vitro	1142:1146	In vitro digestion showed that PSF was minimally digested with the in vitro gastrointestinal tract simulation, but PSF with UV/H2O2 treatment could be digested in the low acid environment in the simulated gastric juice, but was minimally digested with the simulated intestinal juice.
31887897	8	58	with	PSF	1254:1256	arg1	treatment					1271:1279	UV/H2O2 treatment	1263:1279	UV/H2O2 treatment	1263:1279	In vitro digestion showed that PSF was minimally digested with the in vitro gastrointestinal tract simulation, but PSF with UV/H2O2 treatment could be digested in the low acid environment in the simulated gastric juice, but was minimally digested with the simulated intestinal juice.
31887897	2	59	theme	structural	244:253	arg1	changes					255:261	The structural changes	240:261	The structural changes of PSF before and after UV/H2O2 treatment	240:303	The structural changes of PSF before and after UV/H2O2 treatment were analyzed, and molecular weight changes during in vitro digestion were determined.
31887897	7	60	contain	had	1118:1120	arg2	viscosity					1128:1136	lower viscosity	1122:1136	lower viscosity	1122:1136	Rheological studies indicated that PSF with UV/H2O2 treatment had lower viscosity.
31887897	7	60	contain	had	1118:1120	arg1	PSF					1091:1093	PSF	1091:1093	PSF with UV/H2O2 treatment	1091:1116	Rheological studies indicated that PSF with UV/H2O2 treatment had lower viscosity.
31887897	1	61	theme	UV/H2O2	148:154	arg1	effect					138:143	The depolymerization effect	117:143	The depolymerization effect of UV/H2O2 on the polysaccharides from Sargassum fusiforme (PSF), a brown algae,	117:224	The depolymerization effect of UV/H2O2 on the polysaccharides from Sargassum fusiforme (PSF), a brown algae, were studied.
31887897	8	62	theme	tract	1232:1236	arg1	simulation					1238:1247	the in vitro gastrointestinal tract simulation	1202:1247	the in vitro gastrointestinal tract simulation	1202:1247	In vitro digestion showed that PSF was minimally digested with the in vitro gastrointestinal tract simulation, but PSF with UV/H2O2 treatment could be digested in the low acid environment in the simulated gastric juice, but was minimally digested with the simulated intestinal juice.
31887897	6	63	theme	transition	925:934	arg1	imaging					995:1001	The helix-coil transition, X-ray diffraction (XRD) and atomic force microscopy (AFM) imaging	910:1001	The helix-coil transition, X-ray diffraction (XRD) and atomic force microscopy (AFM) imaging	910:1001	The helix-coil transition, X-ray diffraction (XRD) and atomic force microscopy (AFM) imaging showed that the UV/H2O2 treatment depolymerized PSF.
31887897	7	64	theme	lower	1122:1126	arg1	viscosity					1128:1136	lower viscosity	1122:1136	lower viscosity	1122:1136	Rheological studies indicated that PSF with UV/H2O2 treatment had lower viscosity.
31887897	5	65	theme	UV/H2O2	782:788	arg1	treatment					790:798	UV/H2O2 treatment	782:798	UV/H2O2 treatment	782:798	The monosaccharide composition showed that UV/H2O2 treatment could increase the mole percentage of mannose (37.44 %) and decrease the mole percentage of fucose (14.88 %).
31887897	2	66	theme	in	356:357	arg1	digestion					365:373	in vitro digestion	356:373	in vitro digestion	356:373	The structural changes of PSF before and after UV/H2O2 treatment were analyzed, and molecular weight changes during in vitro digestion were determined.
31887897	6	67	theme	atomic	965:970	arg1	AFM					990:992	AFM	990:992	AFM	990:992	The helix-coil transition, X-ray diffraction (XRD) and atomic force microscopy (AFM) imaging showed that the UV/H2O2 treatment depolymerized PSF.
31887897	6	67	theme	atomic	965:970	arg1	microscopy					978:987	atomic force microscopy	965:987	atomic force microscopy (AFM)	965:993	The helix-coil transition, X-ray diffraction (XRD) and atomic force microscopy (AFM) imaging showed that the UV/H2O2 treatment depolymerized PSF.
31887897	6	67	theme	atomic	965:970	arg1	transition					925:934	The helix-coil transition	910:934	The helix-coil transition	910:934	The helix-coil transition, X-ray diffraction (XRD) and atomic force microscopy (AFM) imaging showed that the UV/H2O2 treatment depolymerized PSF.
31887897	6	68	theme	helix-coil	914:923	arg1	microscopy					978:987	atomic force microscopy	965:987	atomic force microscopy (AFM)	965:993	The helix-coil transition, X-ray diffraction (XRD) and atomic force microscopy (AFM) imaging showed that the UV/H2O2 treatment depolymerized PSF.
31887897	6	68	theme	helix-coil	914:923	arg1	transition					925:934	The helix-coil transition	910:934	The helix-coil transition	910:934	The helix-coil transition, X-ray diffraction (XRD) and atomic force microscopy (AFM) imaging showed that the UV/H2O2 treatment depolymerized PSF.
31887897	6	68	theme	helix-coil	914:923	arg1	diffraction					943:953	X-ray diffraction	937:953	X-ray diffraction (XRD)	937:959	The helix-coil transition, X-ray diffraction (XRD) and atomic force microscopy (AFM) imaging showed that the UV/H2O2 treatment depolymerized PSF.
31887897	6	69	theme	UV/H2O2	1019:1025	arg1	treatment					1027:1035	the UV/H2O2 treatment	1015:1035	the UV/H2O2 treatment	1015:1035	The helix-coil transition, X-ray diffraction (XRD) and atomic force microscopy (AFM) imaging showed that the UV/H2O2 treatment depolymerized PSF.
31887897	5	70	theme	monosaccharide	743:756	arg1	composition					758:768	The monosaccharide composition	739:768	The monosaccharide composition	739:768	The monosaccharide composition showed that UV/H2O2 treatment could increase the mole percentage of mannose (37.44 %) and decrease the mole percentage of fucose (14.88 %).
31887897	7	71	theme	Rheological	1056:1066	arg1	studies					1068:1074	Rheological studies	1056:1074	Rheological studies	1056:1074	Rheological studies indicated that PSF with UV/H2O2 treatment had lower viscosity.
31887897	9	72	theme	low	1506:1508	arg1	weight					1520:1525	low molecular weight	1506:1525	low molecular weight	1506:1525	This studied suggested that the preparation and application of functional PSF with low molecular weight might be beneficial.
31887897	7	73	theme	UV/H2O2	1100:1106	arg1	treatment					1108:1116	UV/H2O2 treatment	1100:1116	UV/H2O2 treatment	1100:1116	Rheological studies indicated that PSF with UV/H2O2 treatment had lower viscosity.
30325076	9	0	from	medium	1239:1244	arg1	filters					1257:1263	filters	1257:1263	filters	1257:1263	Bio-based hosting materials of this kind with two different hosting functional groups, ureido and amine, can potentially be utilized for a wide range of applications in aqueous medium, including filters, catalysis and biomedicine.
30325076	9	0	from	medium	1239:1244	arg1	range					1206:1210	a wide range	1199:1210	a wide range of applications in aqueous medium, including filters, catalysis and biomedicine	1199:1290	Bio-based hosting materials of this kind with two different hosting functional groups, ureido and amine, can potentially be utilized for a wide range of applications in aqueous medium, including filters, catalysis and biomedicine.
30325076	1	1	theme	chelating	157:165	arg1	functions					167:175	chelating functions	157:175	chelating functions	157:175	Aerogels of polysaccharides with chelating functions can be useful as supports in many applications because of their hosting properties.
30325076	4	2	theme	ureido	580:585	arg1	groups					598:603	ureido functional groups	580:603	ureido functional groups	580:603	The presence of ureido functional groups was confirmed by FTIR, solid-state CP-MAS 13 C and 15 N NMR analyses.
30325076	7	3	theme	crystalline	939:949	arg1	structure					951:959	a highly ordered crystalline structure	922:959	a highly ordered crystalline structure	922:959	Characterization by powder XRD confirmed that the polymer chains self-assembled, with the chitin polymers oriented in a highly ordered crystalline structure.
30325076	3	4	with	chitosan	517:524	arg1	acid					541:544	isocyanic acid	531:544	isocyanic acid prepared in situ	531:561	The nanofibrillar networks of "ureido-chitin" were produced by the nucleophilic addition of amine groups of chitosan with isocyanic acid prepared in situ.
30325076	9	5	from	applications	1215:1226	arg1	medium					1239:1244	aqueous medium	1231:1244	aqueous medium	1231:1244	Bio-based hosting materials of this kind with two different hosting functional groups, ureido and amine, can potentially be utilized for a wide range of applications in aqueous medium, including filters, catalysis and biomedicine.
30325076	7	6	theme	polymer	854:860	arg1	chains					862:867	the polymer chains	850:867	the polymer chains	850:867	Characterization by powder XRD confirmed that the polymer chains self-assembled, with the chitin polymers oriented in a highly ordered crystalline structure.
30325076	4	7	theme	groups	598:603	arg1	presence					568:575	The presence	564:575	The presence of ureido functional groups	564:603	The presence of ureido functional groups was confirmed by FTIR, solid-state CP-MAS 13 C and 15 N NMR analyses.
30325076	9	8	theme	functional	1130:1139	arg1	groups					1141:1146	two different hosting functional groups	1108:1146	two different hosting functional groups	1108:1146	Bio-based hosting materials of this kind with two different hosting functional groups, ureido and amine, can potentially be utilized for a wide range of applications in aqueous medium, including filters, catalysis and biomedicine.
30325076	9	8	theme	functional	1130:1139	arg1	amine					1160:1164	amine	1160:1164	amine	1160:1164	Bio-based hosting materials of this kind with two different hosting functional groups, ureido and amine, can potentially be utilized for a wide range of applications in aqueous medium, including filters, catalysis and biomedicine.
30325076	9	8	theme	functional	1130:1139	arg1	ureido					1149:1154	ureido	1149:1154	ureido	1149:1154	Bio-based hosting materials of this kind with two different hosting functional groups, ureido and amine, can potentially be utilized for a wide range of applications in aqueous medium, including filters, catalysis and biomedicine.
30325076	2	9	theme	facile	286:291	arg1	method					293:298	a facile method	284:298	a facile method for the preparation of aerogels of chitosan derivative "ureido-chitin", containing ureido functional groups	284:406	This work demonstrates a facile method for the preparation of aerogels of chitosan derivative "ureido-chitin", containing ureido functional groups.
30325076	9	10	theme	hosting	1072:1078	arg1	materials					1080:1088	Bio-based hosting materials	1062:1088	Bio-based hosting materials of this kind with two different hosting functional groups, ureido and amine,	1062:1165	Bio-based hosting materials of this kind with two different hosting functional groups, ureido and amine, can potentially be utilized for a wide range of applications in aqueous medium, including filters, catalysis and biomedicine.
30325076	4	11	theme	functional	587:596	arg1	groups					598:603	ureido functional groups	580:603	ureido functional groups	580:603	The presence of ureido functional groups was confirmed by FTIR, solid-state CP-MAS 13 C and 15 N NMR analyses.
30325076	0	12	theme	Functional	105:114	arg1	Ureido					75:80	Ureido	75:80	Ureido	75:80	Facile Preparation of Nanofibrillar Networks of "Ureido-Chitin" Containing Ureido and Amine as Chelating Functional Groups.
30325076	0	12	theme	Functional	105:114	arg1	Groups					116:121	Chelating Functional Groups	95:121	Chelating Functional Groups	95:121	Facile Preparation of Nanofibrillar Networks of "Ureido-Chitin" Containing Ureido and Amine as Chelating Functional Groups.
30325076	0	12	theme	Functional	105:114	arg1	Amine					86:90	Amine	86:90	Amine	86:90	Facile Preparation of Nanofibrillar Networks of "Ureido-Chitin" Containing Ureido and Amine as Chelating Functional Groups.
30325076	8	13	theme	nanofibrillar	966:978	arg1	networks					980:987	The nanofibrillar networks	962:987	The nanofibrillar networks	962:987	The nanofibrillar networks showed no solubility under either aqueous acidic or alkaline conditions.
30325076	1	14	with	Aerogels	124:131	arg1	functions					167:175	chelating functions	157:175	chelating functions	157:175	Aerogels of polysaccharides with chelating functions can be useful as supports in many applications because of their hosting properties.
30325076	7	15	theme	ordered	931:937	arg1	structure					951:959	a highly ordered crystalline structure	922:959	a highly ordered crystalline structure	922:959	Characterization by powder XRD confirmed that the polymer chains self-assembled, with the chitin polymers oriented in a highly ordered crystalline structure.
30325076	3	16	theme	isocyanic	531:539	arg1	acid					541:544	isocyanic acid	531:544	isocyanic acid prepared in situ	531:561	The nanofibrillar networks of "ureido-chitin" were produced by the nucleophilic addition of amine groups of chitosan with isocyanic acid prepared in situ.
30325076	4	17	theme	N	659:659	arg1	NMR					661:663	solid-state CP-MAS 13 C and 15 N NMR	628:663	NMR	661:663	The presence of ureido functional groups was confirmed by FTIR, solid-state CP-MAS 13 C and 15 N NMR analyses.
30325076	4	17	theme	N	659:659	arg1	FTIR					622:625	FTIR	622:625	FTIR	622:625	The presence of ureido functional groups was confirmed by FTIR, solid-state CP-MAS 13 C and 15 N NMR analyses.
30325076	3	18	theme	ureido-chitin	440:452	arg1	"					453:453	"ureido-chitin"	439:453	"ureido-chitin"	439:453	The nanofibrillar networks of "ureido-chitin" were produced by the nucleophilic addition of amine groups of chitosan with isocyanic acid prepared in situ.
30325076	0	19	theme	Chelating	95:103	arg1	Ureido					75:80	Ureido	75:80	Ureido	75:80	Facile Preparation of Nanofibrillar Networks of "Ureido-Chitin" Containing Ureido and Amine as Chelating Functional Groups.
30325076	0	19	theme	Chelating	95:103	arg1	Groups					116:121	Chelating Functional Groups	95:121	Chelating Functional Groups	95:121	Facile Preparation of Nanofibrillar Networks of "Ureido-Chitin" Containing Ureido and Amine as Chelating Functional Groups.
30325076	0	19	theme	Chelating	95:103	arg1	Amine					86:90	Amine	86:90	Amine	86:90	Facile Preparation of Nanofibrillar Networks of "Ureido-Chitin" Containing Ureido and Amine as Chelating Functional Groups.
30325076	3	20	theme	nanofibrillar	413:425	arg1	networks					427:434	The nanofibrillar networks	409:434	The nanofibrillar networks of "ureido-chitin"	409:453	The nanofibrillar networks of "ureido-chitin" were produced by the nucleophilic addition of amine groups of chitosan with isocyanic acid prepared in situ.
30325076	7	21	theme	powder	824:829	arg1	XRD					831:833	powder XRD	824:833	powder XRD	824:833	Characterization by powder XRD confirmed that the polymer chains self-assembled, with the chitin polymers oriented in a highly ordered crystalline structure.
30325076	2	22	theme	functional	390:399	arg1	groups					401:406	ureido functional groups	383:406	ureido functional groups	383:406	This work demonstrates a facile method for the preparation of aerogels of chitosan derivative "ureido-chitin", containing ureido functional groups.
30325076	9	23	used	utilized	1186:1193	arg2	materials					1080:1088	Bio-based hosting materials	1062:1088	Bio-based hosting materials of this kind with two different hosting functional groups, ureido and amine,	1062:1165	Bio-based hosting materials of this kind with two different hosting functional groups, ureido and amine, can potentially be utilized for a wide range of applications in aqueous medium, including filters, catalysis and biomedicine.
30325076	2	24	theme	ureido	383:388	arg1	groups					401:406	ureido functional groups	383:406	ureido functional groups	383:406	This work demonstrates a facile method for the preparation of aerogels of chitosan derivative "ureido-chitin", containing ureido functional groups.
30325076	0	25	theme	Facile	0:5	arg1	Preparation					7:17	Facile Preparation	0:17	Facile Preparation of Nanofibrillar Networks of "Ureido-Chitin"	0:62	Facile Preparation of Nanofibrillar Networks of "Ureido-Chitin" Containing Ureido and Amine as Chelating Functional Groups.
30325076	9	26	with	materials	1080:1088	arg1	groups					1141:1146	two different hosting functional groups	1108:1146	two different hosting functional groups	1108:1146	Bio-based hosting materials of this kind with two different hosting functional groups, ureido and amine, can potentially be utilized for a wide range of applications in aqueous medium, including filters, catalysis and biomedicine.
30325076	9	26	with	materials	1080:1088	arg1	amine					1160:1164	amine	1160:1164	amine	1160:1164	Bio-based hosting materials of this kind with two different hosting functional groups, ureido and amine, can potentially be utilized for a wide range of applications in aqueous medium, including filters, catalysis and biomedicine.
30325076	9	26	with	materials	1080:1088	arg1	ureido					1149:1154	ureido	1149:1154	ureido	1149:1154	Bio-based hosting materials of this kind with two different hosting functional groups, ureido and amine, can potentially be utilized for a wide range of applications in aqueous medium, including filters, catalysis and biomedicine.
30325076	6	27	theme	groups	765:770	arg1	degree					737:742	The maximum degree	725:742	The maximum degree of ureido functional groups	725:770	The maximum degree of ureido functional groups was estimated to be about 66 %.
30325076	9	28	theme	kind	1098:1101	arg1	materials					1080:1088	Bio-based hosting materials	1062:1088	Bio-based hosting materials of this kind with two different hosting functional groups, ureido and amine,	1062:1165	Bio-based hosting materials of this kind with two different hosting functional groups, ureido and amine, can potentially be utilized for a wide range of applications in aqueous medium, including filters, catalysis and biomedicine.
30325076	0	29	theme	Nanofibrillar	22:34	arg1	Networks					36:43	Nanofibrillar Networks	22:43	Nanofibrillar Networks of "Ureido-Chitin"	22:62	Facile Preparation of Nanofibrillar Networks of "Ureido-Chitin" Containing Ureido and Amine as Chelating Functional Groups.
30325076	9	30	theme	wide	1201:1204	arg1	filters					1257:1263	filters	1257:1263	filters	1257:1263	Bio-based hosting materials of this kind with two different hosting functional groups, ureido and amine, can potentially be utilized for a wide range of applications in aqueous medium, including filters, catalysis and biomedicine.
30325076	9	30	theme	wide	1201:1204	arg1	range					1206:1210	a wide range	1199:1210	a wide range of applications in aqueous medium, including filters, catalysis and biomedicine	1199:1290	Bio-based hosting materials of this kind with two different hosting functional groups, ureido and amine, can potentially be utilized for a wide range of applications in aqueous medium, including filters, catalysis and biomedicine.
30325076	6	31	theme	functional	754:763	arg1	groups					765:770	ureido functional groups	747:770	ureido functional groups	747:770	The maximum degree of ureido functional groups was estimated to be about 66 %.
30325076	4	32	theme	solid-state	628:638	arg1	C					650:650	solid-state CP-MAS 13 C and 15 N NMR	628:663	C	650:650	The presence of ureido functional groups was confirmed by FTIR, solid-state CP-MAS 13 C and 15 N NMR analyses.
30325076	4	32	theme	solid-state	628:638	arg1	FTIR					622:625	FTIR	622:625	FTIR	622:625	The presence of ureido functional groups was confirmed by FTIR, solid-state CP-MAS 13 C and 15 N NMR analyses.
30325076	2	33	contain	containing	372:381	arg1	aerogels					323:330	aerogels	323:330	aerogels	323:330	This work demonstrates a facile method for the preparation of aerogels of chitosan derivative "ureido-chitin", containing ureido functional groups.
30325076	2	33	contain	containing	372:381	arg2	groups					401:406	ureido functional groups	383:406	ureido functional groups	383:406	This work demonstrates a facile method for the preparation of aerogels of chitosan derivative "ureido-chitin", containing ureido functional groups.
30325076	3	34	theme	chitosan	517:524	arg1	chitosan					517:524	chitosan	517:524	chitosan with isocyanic acid prepared in situ	517:561	The nanofibrillar networks of "ureido-chitin" were produced by the nucleophilic addition of amine groups of chitosan with isocyanic acid prepared in situ.
30325076	3	34	theme	chitosan	517:524	arg1	groups					507:512	amine groups	501:512	amine groups of chitosan with isocyanic acid prepared in situ	501:561	The nanofibrillar networks of "ureido-chitin" were produced by the nucleophilic addition of amine groups of chitosan with isocyanic acid prepared in situ.
30325076	3	35	theme	groups	507:512	arg1	addition					489:496	the nucleophilic addition	472:496	the nucleophilic addition of amine groups of chitosan with isocyanic acid prepared in situ	472:561	The nanofibrillar networks of "ureido-chitin" were produced by the nucleophilic addition of amine groups of chitosan with isocyanic acid prepared in situ.
30325076	3	36	theme	amine	501:505	arg1	chitosan					517:524	chitosan	517:524	chitosan with isocyanic acid prepared in situ	517:561	The nanofibrillar networks of "ureido-chitin" were produced by the nucleophilic addition of amine groups of chitosan with isocyanic acid prepared in situ.
30325076	3	36	theme	amine	501:505	arg1	groups					507:512	amine groups	501:512	amine groups of chitosan with isocyanic acid prepared in situ	501:561	The nanofibrillar networks of "ureido-chitin" were produced by the nucleophilic addition of amine groups of chitosan with isocyanic acid prepared in situ.
30325076	5	37	theme	molecular	694:702	arg1	chains					704:709	molecular chains	694:709	molecular chains	694:709	No crosslinking of molecular chains was observed.
30325076	9	38	theme	applications	1215:1226	arg1	filters					1257:1263	filters	1257:1263	filters	1257:1263	Bio-based hosting materials of this kind with two different hosting functional groups, ureido and amine, can potentially be utilized for a wide range of applications in aqueous medium, including filters, catalysis and biomedicine.
30325076	9	38	theme	applications	1215:1226	arg1	range					1206:1210	a wide range	1199:1210	a wide range of applications in aqueous medium, including filters, catalysis and biomedicine	1199:1290	Bio-based hosting materials of this kind with two different hosting functional groups, ureido and amine, can potentially be utilized for a wide range of applications in aqueous medium, including filters, catalysis and biomedicine.
30325076	9	39	theme	different	1112:1120	arg1	groups					1141:1146	two different hosting functional groups	1108:1146	two different hosting functional groups	1108:1146	Bio-based hosting materials of this kind with two different hosting functional groups, ureido and amine, can potentially be utilized for a wide range of applications in aqueous medium, including filters, catalysis and biomedicine.
30325076	9	39	theme	different	1112:1120	arg1	amine					1160:1164	amine	1160:1164	amine	1160:1164	Bio-based hosting materials of this kind with two different hosting functional groups, ureido and amine, can potentially be utilized for a wide range of applications in aqueous medium, including filters, catalysis and biomedicine.
30325076	9	39	theme	different	1112:1120	arg1	ureido					1149:1154	ureido	1149:1154	ureido	1149:1154	Bio-based hosting materials of this kind with two different hosting functional groups, ureido and amine, can potentially be utilized for a wide range of applications in aqueous medium, including filters, catalysis and biomedicine.
30325076	1	40	theme	many	206:209	arg1	applications					211:222	many applications	206:222	many applications	206:222	Aerogels of polysaccharides with chelating functions can be useful as supports in many applications because of their hosting properties.
30325076	0	41	theme	Networks	36:43	arg1	Preparation					7:17	Facile Preparation	0:17	Facile Preparation of Nanofibrillar Networks of "Ureido-Chitin"	0:62	Facile Preparation of Nanofibrillar Networks of "Ureido-Chitin" Containing Ureido and Amine as Chelating Functional Groups.
30325076	2	42	theme	ureido-chitin	356:368	arg1	"					369:369	chitosan derivative "ureido-chitin"	335:369	chitosan derivative "ureido-chitin"	335:369	This work demonstrates a facile method for the preparation of aerogels of chitosan derivative "ureido-chitin", containing ureido functional groups.
30325076	5	43	theme	chains	704:709	arg1	crosslinking					678:689	No crosslinking	675:689	No crosslinking of molecular chains	675:709	No crosslinking of molecular chains was observed.
30325076	6	44	theme	ureido	747:752	arg1	groups					765:770	ureido functional groups	747:770	ureido functional groups	747:770	The maximum degree of ureido functional groups was estimated to be about 66 %.
30325076	4	45	theme	CP-MAS	640:645	arg1	C					650:650	solid-state CP-MAS 13 C and 15 N NMR	628:663	C	650:650	The presence of ureido functional groups was confirmed by FTIR, solid-state CP-MAS 13 C and 15 N NMR analyses.
30325076	4	45	theme	CP-MAS	640:645	arg1	FTIR					622:625	FTIR	622:625	FTIR	622:625	The presence of ureido functional groups was confirmed by FTIR, solid-state CP-MAS 13 C and 15 N NMR analyses.
30325076	0	46	theme	Ureido-Chitin	49:61	arg1	"					62:62	"Ureido-Chitin"	48:62	"Ureido-Chitin"	48:62	Facile Preparation of Nanofibrillar Networks of "Ureido-Chitin" Containing Ureido and Amine as Chelating Functional Groups.
30325076	9	47	theme	aqueous	1231:1237	arg1	medium					1239:1244	aqueous medium	1231:1244	aqueous medium	1231:1244	Bio-based hosting materials of this kind with two different hosting functional groups, ureido and amine, can potentially be utilized for a wide range of applications in aqueous medium, including filters, catalysis and biomedicine.
30325076	1	48	from	applications	211:222	arg1	useful					184:189	useful	184:189	useful	184:189	Aerogels of polysaccharides with chelating functions can be useful as supports in many applications because of their hosting properties.
30325076	2	49	theme	derivative	344:353	arg1	"					369:369	chitosan derivative "ureido-chitin"	335:369	chitosan derivative "ureido-chitin"	335:369	This work demonstrates a facile method for the preparation of aerogels of chitosan derivative "ureido-chitin", containing ureido functional groups.
30325076	4	50	dep	FTIR	622:625	arg1	analyses					665:672	analyses	665:672	analyses	665:672	The presence of ureido functional groups was confirmed by FTIR, solid-state CP-MAS 13 C and 15 N NMR analyses.
30325076	8	51	theme	alkaline	1041:1048	arg1	conditions					1050:1059	alkaline conditions	1041:1059	alkaline conditions	1041:1059	The nanofibrillar networks showed no solubility under either aqueous acidic or alkaline conditions.
30325076	2	52	theme	chitosan	335:342	arg1	"					369:369	chitosan derivative "ureido-chitin"	335:369	chitosan derivative "ureido-chitin"	335:369	This work demonstrates a facile method for the preparation of aerogels of chitosan derivative "ureido-chitin", containing ureido functional groups.
30325076	6	53	theme	maximum	729:735	arg1	degree					737:742	The maximum degree	725:742	The maximum degree of ureido functional groups	725:770	The maximum degree of ureido functional groups was estimated to be about 66 %.
30325076	3	54	theme	nucleophilic	476:487	arg1	addition					489:496	the nucleophilic addition	472:496	the nucleophilic addition of amine groups of chitosan with isocyanic acid prepared in situ	472:561	The nanofibrillar networks of "ureido-chitin" were produced by the nucleophilic addition of amine groups of chitosan with isocyanic acid prepared in situ.
30325076	7	55	theme	chitin	894:899	arg1	polymers					901:908	the chitin polymers	890:908	the chitin polymers oriented in a highly ordered crystalline structure	890:959	Characterization by powder XRD confirmed that the polymer chains self-assembled, with the chitin polymers oriented in a highly ordered crystalline structure.
30325076	9	56	theme	hosting	1122:1128	arg1	groups					1141:1146	two different hosting functional groups	1108:1146	two different hosting functional groups	1108:1146	Bio-based hosting materials of this kind with two different hosting functional groups, ureido and amine, can potentially be utilized for a wide range of applications in aqueous medium, including filters, catalysis and biomedicine.
30325076	9	56	theme	hosting	1122:1128	arg1	amine					1160:1164	amine	1160:1164	amine	1160:1164	Bio-based hosting materials of this kind with two different hosting functional groups, ureido and amine, can potentially be utilized for a wide range of applications in aqueous medium, including filters, catalysis and biomedicine.
30325076	9	56	theme	hosting	1122:1128	arg1	ureido					1149:1154	ureido	1149:1154	ureido	1149:1154	Bio-based hosting materials of this kind with two different hosting functional groups, ureido and amine, can potentially be utilized for a wide range of applications in aqueous medium, including filters, catalysis and biomedicine.
30325076	1	57	from	useful	184:189	arg1	applications					211:222	many applications	206:222	many applications	206:222	Aerogels of polysaccharides with chelating functions can be useful as supports in many applications because of their hosting properties.
30325076	2	58	theme	aerogels	323:330	arg1	preparation					308:318	the preparation	304:318	the preparation of aerogels of chitosan derivative "ureido-chitin", containing ureido functional groups	304:406	This work demonstrates a facile method for the preparation of aerogels of chitosan derivative "ureido-chitin", containing ureido functional groups.
30325076	9	59	theme	Bio-based	1062:1070	arg1	materials					1080:1088	Bio-based hosting materials	1062:1088	Bio-based hosting materials of this kind with two different hosting functional groups, ureido and amine,	1062:1165	Bio-based hosting materials of this kind with two different hosting functional groups, ureido and amine, can potentially be utilized for a wide range of applications in aqueous medium, including filters, catalysis and biomedicine.
30325076	1	60	theme	polysaccharides	136:150	arg1	Aerogels					124:131	Aerogels	124:131	Aerogels of polysaccharides with chelating functions	124:175	Aerogels of polysaccharides with chelating functions can be useful as supports in many applications because of their hosting properties.
30325076	3	61	theme	"	453:453	arg1	networks					427:434	The nanofibrillar networks	409:434	The nanofibrillar networks of "ureido-chitin"	409:453	The nanofibrillar networks of "ureido-chitin" were produced by the nucleophilic addition of amine groups of chitosan with isocyanic acid prepared in situ.
30325076	0	62	theme	"	62:62	arg1	Networks					36:43	Nanofibrillar Networks	22:43	Nanofibrillar Networks of "Ureido-Chitin"	22:62	Facile Preparation of Nanofibrillar Networks of "Ureido-Chitin" Containing Ureido and Amine as Chelating Functional Groups.
30325076	9	63	from	range	1206:1210	arg1	medium					1239:1244	aqueous medium	1231:1244	aqueous medium	1231:1244	Bio-based hosting materials of this kind with two different hosting functional groups, ureido and amine, can potentially be utilized for a wide range of applications in aqueous medium, including filters, catalysis and biomedicine.
30325076	2	64	theme	"	369:369	arg1	aerogels					323:330	aerogels	323:330	aerogels	323:330	This work demonstrates a facile method for the preparation of aerogels of chitosan derivative "ureido-chitin", containing ureido functional groups.
30325076	1	65	theme	hosting	241:247	arg1	properties					249:258	their hosting properties	235:258	their hosting properties	235:258	Aerogels of polysaccharides with chelating functions can be useful as supports in many applications because of their hosting properties.
31779692	5	0	theme	OECD	680:683	arg1	No.425					695:700	OECD Guideline No.425	680:700	OECD Guideline No.425	680:700	METHODS Acute toxicity of micronized PGA (m-PGA) was assessed in rats following OECD Guideline No.425.
31779692	10	1	theme	post-injection	1231:1244	arg1	points					1251:1256	different post-injection time points	1221:1256	different post-injection time points	1221:1256	Mechanical allodynia and motor function were evaluated at different post-injection time points.
31779692	8	2	dep	post-injection	909:922	arg1	up					899:900	up	899:900	up	899:900	Paw edema and thermal hyperalgesia were measured up to 6 h post-injection, when also myeloperoxidase activity and histological inflammation score were assessed.
31779692	2	3	theme	mast	302:305	arg1	cells					307:311	Joint mast cells	296:311	Joint mast cells	296:311	Joint mast cells are considered to play a key role in orchestrating this detrimental triad.
31779692	12	4	theme	MMP-1	1439:1443	arg1	Levels					1406:1411	Levels	1406:1411	Levels of TNF-α, IL-1β, NGF, and MMP-1, MMP-3, and MMP-9	1406:1461	Levels of TNF-α, IL-1β, NGF, and MMP-1, MMP-3, and MMP-9 were measured in serum using commercial colorimetric ELISA kits.
31779692	10	5	theme	time	1246:1249	arg1	points					1251:1256	different post-injection time points	1221:1256	different post-injection time points	1221:1256	Mechanical allodynia and motor function were evaluated at different post-injection time points.
31779692	17	6	theme	cell	2094:2097	arg1	count					2099:2103	mast cell count	2089:2103	mast cell count	2089:2103	The MIA-induced increase in mast cell count and serum level of the investigated markers was also counteracted by PGA and to a significantly greater extent by m-PGA.
31779692	6	7	theme	-injected	787:795	arg1	rats					797:800	carrageenan (CAR)-injected rats	770:800	carrageenan (CAR)-injected rats	770:800	PGA and m-PGA (30 mg/kg and 100 mg/kg) were orally administered to carrageenan (CAR)-injected rats.
31779692	0	8	theme	inflammation	114:125	arg1	models					104:109	preclinical models	92:109	preclinical models of inflammation	92:125	Safety and efficacy of a new micronized formulation of the ALIAmide palmitoylglucosamine in preclinical models of inflammation and osteoarthritis pain.
31779692	13	9	theme	multiple	1593:1600	arg1	comparisons					1602:1612	multiple comparisons	1593:1612	multiple comparisons	1593:1612	One- or two-way ANOVA followed by a Bonferroni post hoc test for multiple comparisons was used.
31779692	19	10	theme	size	2403:2406	arg1	reduction					2408:2416	particle size reduction	2394:2416	particle size reduction	2394:2416	Moreover, it proved that particle size reduction greatly enhances the activity of PGA, particularly on joint pain and disability.
31779692	18	11	theme	joint-protective	2343:2358	arg1	effects					2360:2366	anti-inflammatory, pain-relieving, and joint-protective effects	2304:2366	anti-inflammatory, pain-relieving, and joint-protective effects	2304:2366	CONCLUSIONS The results of the present study showed that PGA is endorsed with anti-inflammatory, pain-relieving, and joint-protective effects.
31779692	9	12	theme	intra-articular	1029:1043	arg1	injection					1045:1053	intra-articular injection	1029:1053	intra-articular injection of sodium monoiodoacetate (MIA)	1029:1085	Rats subjected to intra-articular injection of sodium monoiodoacetate (MIA) were treated three times per week for 21 days with PGA or m-PGA (30 mg/kg).
31779692	14	13	theme	2000 mg/kg	1698:1707	arg1	excess					1688:1693	excess	1688:1693	excess of 2000 mg/kg	1688:1707	RESULTS Acute oral toxicity of m-PGA resulted in LD50 values in excess of 2000 mg/kg.
31779692	1	14	theme	complex	224:230	arg1	interplay					232:240	a complex interplay	222:240	a complex interplay between inflammation, chrondrodegeneration, and pain	222:293	BACKGROUND Osteoarthritis is increasingly recognized as the result of a complex interplay between inflammation, chrondrodegeneration, and pain.
31779692	13	15	theme	post	1575:1578	arg1	test					1584:1587	a Bonferroni post hoc test	1562:1587	a Bonferroni post hoc test for multiple comparisons	1562:1612	One- or two-way ANOVA followed by a Bonferroni post hoc test for multiple comparisons was used.
31779692	16	16	theme	histologic	2025:2034	arg1	damage					2053:2058	histologic and radiographic damage	2025:2058	damage	2053:2058	Micronized PGA resulted in a superior activity to PGA on MIA-induced mechanical allodynia, locomotor disability, and histologic and radiographic damage.
31779692	9	17	theme	monoiodoacetate	1065:1079	arg1	injection					1045:1053	intra-articular injection	1029:1053	intra-articular injection of sodium monoiodoacetate (MIA)	1029:1085	Rats subjected to intra-articular injection of sodium monoiodoacetate (MIA) were treated three times per week for 21 days with PGA or m-PGA (30 mg/kg).
31779692	13	18	theme	two-way	1536:1542	arg1	ANOVA					1544:1548	two-way ANOVA	1536:1548	two-way ANOVA	1536:1548	One- or two-way ANOVA followed by a Bonferroni post hoc test for multiple comparisons was used.
31779692	8	19	theme	Paw	850:852	arg1	edema					854:858	Paw edema	850:858	Paw edema	850:858	Paw edema and thermal hyperalgesia were measured up to 6 h post-injection, when also myeloperoxidase activity and histological inflammation score were assessed.
31779692	1	20	theme	BACKGROUND	152:161	arg1	Osteoarthritis					163:176	BACKGROUND Osteoarthritis	152:176	BACKGROUND Osteoarthritis	152:176	BACKGROUND Osteoarthritis is increasingly recognized as the result of a complex interplay between inflammation, chrondrodegeneration, and pain.
31779692	1	20	theme	BACKGROUND	152:161	arg1	result					212:217	the result	208:217	the result of a complex interplay between inflammation, chrondrodegeneration, and pain	208:293	BACKGROUND Osteoarthritis is increasingly recognized as the result of a complex interplay between inflammation, chrondrodegeneration, and pain.
31779692	0	21	from	efficacy	11:18	arg1	models					104:109	preclinical models	92:109	preclinical models of inflammation	92:125	Safety and efficacy of a new micronized formulation of the ALIAmide palmitoylglucosamine in preclinical models of inflammation and osteoarthritis pain.
31779692	11	22	theme	articular	1314:1322	arg1	cells					1329:1333	articular mast cells	1314:1333	articular mast cells	1314:1333	Joint histological and radiographic damage was scored, articular mast cells were counted, and macrophages were immunohistochemically investigated.
31779692	17	23	theme	MIA-induced	2065:2075	arg1	increase					2077:2084	The MIA-induced increase	2061:2084	The MIA-induced increase in mast cell count and serum level of the investigated markers	2061:2147	The MIA-induced increase in mast cell count and serum level of the investigated markers was also counteracted by PGA and to a significantly greater extent by m-PGA.
31779692	0	24	theme	ALIAmide	59:66	arg1	palmitoylglucosamine					68:87	the ALIAmide palmitoylglucosamine	55:87	the ALIAmide palmitoylglucosamine	55:87	Safety and efficacy of a new micronized formulation of the ALIAmide palmitoylglucosamine in preclinical models of inflammation and osteoarthritis pain.
31779692	14	25	theme	m-PGA	1655:1659	arg1	toxicity					1643:1650	Acute oral toxicity	1632:1650	Acute oral toxicity of m-PGA	1632:1659	RESULTS Acute oral toxicity of m-PGA resulted in LD50 values in excess of 2000 mg/kg.
31779692	5	26	theme	PGA	637:639	arg1	toxicity					614:621	METHODS Acute toxicity	600:621	METHODS Acute toxicity of micronized PGA (m-PGA)	600:647	METHODS Acute toxicity of micronized PGA (m-PGA) was assessed in rats following OECD Guideline No.425.
31779692	0	27	from	Safety	0:5	arg1	models					104:109	preclinical models	92:109	preclinical models of inflammation	92:125	Safety and efficacy of a new micronized formulation of the ALIAmide palmitoylglucosamine in preclinical models of inflammation and osteoarthritis pain.
31779692	15	28	theme	oral	1719:1722	arg1	administration					1724:1737	A single oral administration	1710:1737	A single oral administration of PGA and m-PGA	1710:1754	A single oral administration of PGA and m-PGA significantly reduced CAR-induced inflammatory signs (edema, inflammatory infiltrate, and hyperalgesia), and m-PGA also reduced the histological score.
31779692	16	29	theme	MIA-induced	1965:1975	arg1	allodynia					1988:1996	MIA-induced mechanical allodynia	1965:1996	MIA-induced mechanical allodynia	1965:1996	Micronized PGA resulted in a superior activity to PGA on MIA-induced mechanical allodynia, locomotor disability, and histologic and radiographic damage.
31779692	15	30	theme	inflammatory	1817:1828	arg1	infiltrate					1830:1839	inflammatory infiltrate	1817:1839	inflammatory infiltrate	1817:1839	A single oral administration of PGA and m-PGA significantly reduced CAR-induced inflammatory signs (edema, inflammatory infiltrate, and hyperalgesia), and m-PGA also reduced the histological score.
31779692	15	30	theme	inflammatory	1817:1828	arg1	signs					1803:1807	CAR-induced inflammatory signs	1778:1807	CAR-induced inflammatory signs (edema, inflammatory infiltrate, and hyperalgesia)	1778:1858	A single oral administration of PGA and m-PGA significantly reduced CAR-induced inflammatory signs (edema, inflammatory infiltrate, and hyperalgesia), and m-PGA also reduced the histological score.
31779692	14	31	theme	Acute	1632:1636	arg1	toxicity					1643:1650	Acute oral toxicity	1632:1650	Acute oral toxicity of m-PGA	1632:1659	RESULTS Acute oral toxicity of m-PGA resulted in LD50 values in excess of 2000 mg/kg.
31779692	5	32	theme	Acute	608:612	arg1	toxicity					614:621	METHODS Acute toxicity	600:621	METHODS Acute toxicity of micronized PGA (m-PGA)	600:647	METHODS Acute toxicity of micronized PGA (m-PGA) was assessed in rats following OECD Guideline No.425.
31779692	17	33	theme	greater	2201:2207	arg1	extent					2209:2214	a significantly greater extent	2185:2214	a significantly greater extent	2185:2214	The MIA-induced increase in mast cell count and serum level of the investigated markers was also counteracted by PGA and to a significantly greater extent by m-PGA.
31779692	3	34	theme	hyperactive	442:452	arg1	cells					454:458	hyperactive cells	442:458	hyperactive cells	442:458	ALIAmides down-modulate mast cells and more generally hyperactive cells.
31779692	16	35	theme	locomotor	1999:2007	arg1	disability					2009:2018	locomotor disability	1999:2018	locomotor disability	1999:2018	Micronized PGA resulted in a superior activity to PGA on MIA-induced mechanical allodynia, locomotor disability, and histologic and radiographic damage.
31779692	11	36	theme	Joint	1259:1263	arg1	damage					1295:1300	Joint histological and radiographic damage	1259:1300	Joint histological and radiographic damage	1259:1300	Joint histological and radiographic damage was scored, articular mast cells were counted, and macrophages were immunohistochemically investigated.
31779692	16	37	from	PGA	1958:1960	arg1	allodynia					1988:1996	MIA-induced mechanical allodynia	1965:1996	MIA-induced mechanical allodynia	1965:1996	Micronized PGA resulted in a superior activity to PGA on MIA-induced mechanical allodynia, locomotor disability, and histologic and radiographic damage.
31779692	16	37	from	PGA	1958:1960	arg1	disability					2009:2018	locomotor disability	1999:2018	locomotor disability	1999:2018	Micronized PGA resulted in a superior activity to PGA on MIA-induced mechanical allodynia, locomotor disability, and histologic and radiographic damage.
31779692	16	37	from	PGA	1958:1960	arg1	damage					2053:2058	histologic and radiographic damage	2025:2058	damage	2053:2058	Micronized PGA resulted in a superior activity to PGA on MIA-induced mechanical allodynia, locomotor disability, and histologic and radiographic damage.
31779692	7	38	theme	Dexamethasone	803:815	arg1	0.1 mg/kg					817:825	Dexamethasone 0.1 mg/kg	803:825	Dexamethasone 0.1 mg/kg	803:825	Dexamethasone 0.1 mg/kg was used as reference.
31779692	7	38	theme	Dexamethasone	803:815	arg1	reference					839:847	reference	839:847	reference	839:847	Dexamethasone 0.1 mg/kg was used as reference.
31779692	18	39	theme	anti-inflammatory	2304:2320	arg1	effects					2360:2366	anti-inflammatory, pain-relieving, and joint-protective effects	2304:2366	anti-inflammatory, pain-relieving, and joint-protective effects	2304:2366	CONCLUSIONS The results of the present study showed that PGA is endorsed with anti-inflammatory, pain-relieving, and joint-protective effects.
31779692	15	40	theme	CAR-induced	1778:1788	arg1	edema					1810:1814	edema	1810:1814	edema	1810:1814	A single oral administration of PGA and m-PGA significantly reduced CAR-induced inflammatory signs (edema, inflammatory infiltrate, and hyperalgesia), and m-PGA also reduced the histological score.
31779692	15	40	theme	CAR-induced	1778:1788	arg1	infiltrate					1830:1839	inflammatory infiltrate	1817:1839	inflammatory infiltrate	1817:1839	A single oral administration of PGA and m-PGA significantly reduced CAR-induced inflammatory signs (edema, inflammatory infiltrate, and hyperalgesia), and m-PGA also reduced the histological score.
31779692	15	40	theme	CAR-induced	1778:1788	arg1	signs					1803:1807	CAR-induced inflammatory signs	1778:1807	CAR-induced inflammatory signs (edema, inflammatory infiltrate, and hyperalgesia)	1778:1858	A single oral administration of PGA and m-PGA significantly reduced CAR-induced inflammatory signs (edema, inflammatory infiltrate, and hyperalgesia), and m-PGA also reduced the histological score.
31779692	15	40	theme	CAR-induced	1778:1788	arg1	hyperalgesia					1846:1857	hyperalgesia	1846:1857	hyperalgesia	1846:1857	A single oral administration of PGA and m-PGA significantly reduced CAR-induced inflammatory signs (edema, inflammatory infiltrate, and hyperalgesia), and m-PGA also reduced the histological score.
31779692	8	41	theme	myeloperoxidase	935:949	arg1	activity					951:958	myeloperoxidase activity	935:958	myeloperoxidase activity	935:958	Paw edema and thermal hyperalgesia were measured up to 6 h post-injection, when also myeloperoxidase activity and histological inflammation score were assessed.
31779692	17	42	theme	markers	2141:2147	arg1	count					2099:2103	mast cell count	2089:2103	mast cell count	2089:2103	The MIA-induced increase in mast cell count and serum level of the investigated markers was also counteracted by PGA and to a significantly greater extent by m-PGA.
31779692	17	42	theme	markers	2141:2147	arg1	level					2115:2119	serum level	2109:2119	serum level	2109:2119	The MIA-induced increase in mast cell count and serum level of the investigated markers was also counteracted by PGA and to a significantly greater extent by m-PGA.
31779692	0	43	theme	micronized	29:38	arg1	formulation					40:50	a new micronized formulation	23:50	a new micronized formulation of the ALIAmide palmitoylglucosamine	23:87	Safety and efficacy of a new micronized formulation of the ALIAmide palmitoylglucosamine in preclinical models of inflammation and osteoarthritis pain.
31779692	10	44	theme	Mechanical	1163:1172	arg1	allodynia					1174:1182	Mechanical allodynia	1163:1182	Mechanical allodynia	1163:1182	Mechanical allodynia and motor function were evaluated at different post-injection time points.
31779692	2	45	theme	key	338:340	arg1	role					342:345	a key role	336:345	a key role	336:345	Joint mast cells are considered to play a key role in orchestrating this detrimental triad.
31779692	1	46	theme	interplay	232:240	arg1	Osteoarthritis					163:176	BACKGROUND Osteoarthritis	152:176	BACKGROUND Osteoarthritis	152:176	BACKGROUND Osteoarthritis is increasingly recognized as the result of a complex interplay between inflammation, chrondrodegeneration, and pain.
31779692	1	46	theme	interplay	232:240	arg1	result					212:217	the result	208:217	the result of a complex interplay between inflammation, chrondrodegeneration, and pain	208:293	BACKGROUND Osteoarthritis is increasingly recognized as the result of a complex interplay between inflammation, chrondrodegeneration, and pain.
31779692	12	47	theme	colorimetric	1503:1514	arg1	kits					1522:1525	commercial colorimetric ELISA kits	1492:1525	commercial colorimetric ELISA kits	1492:1525	Levels of TNF-α, IL-1β, NGF, and MMP-1, MMP-3, and MMP-9 were measured in serum using commercial colorimetric ELISA kits.
31779692	8	48	theme	inflammation	977:988	arg1	score					990:994	histological inflammation score	964:994	histological inflammation score	964:994	Paw edema and thermal hyperalgesia were measured up to 6 h post-injection, when also myeloperoxidase activity and histological inflammation score were assessed.
31779692	0	49	from	osteoarthritis	131:144	arg1	models					104:109	preclinical models	92:109	preclinical models of inflammation	92:125	Safety and efficacy of a new micronized formulation of the ALIAmide palmitoylglucosamine in preclinical models of inflammation and osteoarthritis pain.
31779692	18	50	theme	present	2257:2263	arg1	study					2265:2269	the present study	2253:2269	the present study	2253:2269	CONCLUSIONS The results of the present study showed that PGA is endorsed with anti-inflammatory, pain-relieving, and joint-protective effects.
31779692	14	51	dep	RESULTS	1624:1630	arg1	resulted					1661:1668	resulted	1661:1668	resulted in LD50 values in excess of 2000 mg/kg	1661:1707	RESULTS Acute oral toxicity of m-PGA resulted in LD50 values in excess of 2000 mg/kg.
31779692	16	52	theme	Micronized	1908:1917	arg1	PGA					1919:1921	Micronized PGA	1908:1921	Micronized PGA	1908:1921	Micronized PGA resulted in a superior activity to PGA on MIA-induced mechanical allodynia, locomotor disability, and histologic and radiographic damage.
31779692	13	53	used	used	1618:1621	arg2	One-					1528:1531	One-	1528:1531	One-	1528:1531	One- or two-way ANOVA followed by a Bonferroni post hoc test for multiple comparisons was used.
31779692	13	53	used	used	1618:1621	arg2	ANOVA					1544:1548	two-way ANOVA	1536:1548	two-way ANOVA	1536:1548	One- or two-way ANOVA followed by a Bonferroni post hoc test for multiple comparisons was used.
31779692	4	54	dep	safety	486:491	arg1	the					482:484	the	482:484	the	482:484	Here we investigated the safety and effectiveness of the ALIAmide N-palmitoyl-D-glucosamine (PGA) in inflammation and osteoarthritis pain.
31779692	4	55	theme	ALIAmide	518:525	arg1	N-palmitoyl-D-glucosamine					527:551	the ALIAmide N-palmitoyl-D-glucosamine	514:551	the ALIAmide N-palmitoyl-D-glucosamine (PGA)	514:557	Here we investigated the safety and effectiveness of the ALIAmide N-palmitoyl-D-glucosamine (PGA) in inflammation and osteoarthritis pain.
31779692	4	55	theme	ALIAmide	518:525	arg1	PGA					554:556	PGA	554:556	PGA	554:556	Here we investigated the safety and effectiveness of the ALIAmide N-palmitoyl-D-glucosamine (PGA) in inflammation and osteoarthritis pain.
31779692	5	56	theme	Guideline	685:693	arg1	No.425					695:700	OECD Guideline No.425	680:700	OECD Guideline No.425	680:700	METHODS Acute toxicity of micronized PGA (m-PGA) was assessed in rats following OECD Guideline No.425.
31779692	17	57	from	increase	2077:2084	arg1	count					2099:2103	mast cell count	2089:2103	mast cell count	2089:2103	The MIA-induced increase in mast cell count and serum level of the investigated markers was also counteracted by PGA and to a significantly greater extent by m-PGA.
31779692	17	57	from	increase	2077:2084	arg1	level					2115:2119	serum level	2109:2119	serum level	2109:2119	The MIA-induced increase in mast cell count and serum level of the investigated markers was also counteracted by PGA and to a significantly greater extent by m-PGA.
31779692	10	58	theme	different	1221:1229	arg1	points					1251:1256	different post-injection time points	1221:1256	different post-injection time points	1221:1256	Mechanical allodynia and motor function were evaluated at different post-injection time points.
31779692	18	59	dep	CONCLUSIONS	2226:2236	arg1	results					2242:2248	The results	2238:2248	CONCLUSIONS The results of the present study	2226:2269	CONCLUSIONS The results of the present study showed that PGA is endorsed with anti-inflammatory, pain-relieving, and joint-protective effects.
31779692	12	60	theme	NGF	1430:1432	arg1	Levels					1406:1411	Levels	1406:1411	Levels of TNF-α, IL-1β, NGF, and MMP-1, MMP-3, and MMP-9	1406:1461	Levels of TNF-α, IL-1β, NGF, and MMP-1, MMP-3, and MMP-9 were measured in serum using commercial colorimetric ELISA kits.
31779692	2	61	theme	Joint	296:300	arg1	cells					307:311	Joint mast cells	296:311	Joint mast cells	296:311	Joint mast cells are considered to play a key role in orchestrating this detrimental triad.
31779692	19	62	theme	particle	2394:2401	arg1	reduction					2408:2416	particle size reduction	2394:2416	particle size reduction	2394:2416	Moreover, it proved that particle size reduction greatly enhances the activity of PGA, particularly on joint pain and disability.
31779692	17	63	theme	mast	2089:2092	arg1	count					2099:2103	mast cell count	2089:2103	mast cell count	2089:2103	The MIA-induced increase in mast cell count and serum level of the investigated markers was also counteracted by PGA and to a significantly greater extent by m-PGA.
31779692	4	64	from	effectiveness	497:509	arg1	inflammation					562:573	inflammation	562:573	inflammation	562:573	Here we investigated the safety and effectiveness of the ALIAmide N-palmitoyl-D-glucosamine (PGA) in inflammation and osteoarthritis pain.
31779692	4	64	from	effectiveness	497:509	arg1	osteoarthritis					579:592	osteoarthritis	579:592	osteoarthritis	579:592	Here we investigated the safety and effectiveness of the ALIAmide N-palmitoyl-D-glucosamine (PGA) in inflammation and osteoarthritis pain.
31779692	13	65	theme	hoc	1580:1582	arg1	test					1584:1587	a Bonferroni post hoc test	1562:1587	a Bonferroni post hoc test for multiple comparisons	1562:1612	One- or two-way ANOVA followed by a Bonferroni post hoc test for multiple comparisons was used.
31779692	12	66	theme	TNF-α	1416:1420	arg1	Levels					1406:1411	Levels	1406:1411	Levels of TNF-α, IL-1β, NGF, and MMP-1, MMP-3, and MMP-9	1406:1461	Levels of TNF-α, IL-1β, NGF, and MMP-1, MMP-3, and MMP-9 were measured in serum using commercial colorimetric ELISA kits.
31779692	4	67	from	safety	486:491	arg1	inflammation					562:573	inflammation	562:573	inflammation	562:573	Here we investigated the safety and effectiveness of the ALIAmide N-palmitoyl-D-glucosamine (PGA) in inflammation and osteoarthritis pain.
31779692	4	67	from	safety	486:491	arg1	osteoarthritis					579:592	osteoarthritis	579:592	osteoarthritis	579:592	Here we investigated the safety and effectiveness of the ALIAmide N-palmitoyl-D-glucosamine (PGA) in inflammation and osteoarthritis pain.
31779692	12	68	theme	IL-1β	1423:1427	arg1	Levels					1406:1411	Levels	1406:1411	Levels of TNF-α, IL-1β, NGF, and MMP-1, MMP-3, and MMP-9	1406:1461	Levels of TNF-α, IL-1β, NGF, and MMP-1, MMP-3, and MMP-9 were measured in serum using commercial colorimetric ELISA kits.
31779692	17	69	theme	serum	2109:2113	arg1	level					2115:2119	serum level	2109:2119	serum level	2109:2119	The MIA-induced increase in mast cell count and serum level of the investigated markers was also counteracted by PGA and to a significantly greater extent by m-PGA.
31779692	14	70	from	values	1678:1683	arg1	excess					1688:1693	excess	1688:1693	excess of 2000 mg/kg	1688:1707	RESULTS Acute oral toxicity of m-PGA resulted in LD50 values in excess of 2000 mg/kg.
31779692	13	71	theme	Bonferroni	1564:1573	arg1	test					1584:1587	a Bonferroni post hoc test	1562:1587	a Bonferroni post hoc test for multiple comparisons	1562:1612	One- or two-way ANOVA followed by a Bonferroni post hoc test for multiple comparisons was used.
31779692	18	72	theme	pain-relieving	2323:2336	arg1	effects					2360:2366	anti-inflammatory, pain-relieving, and joint-protective effects	2304:2366	anti-inflammatory, pain-relieving, and joint-protective effects	2304:2366	CONCLUSIONS The results of the present study showed that PGA is endorsed with anti-inflammatory, pain-relieving, and joint-protective effects.
31779692	15	73	theme	m-PGA	1750:1754	arg1	administration					1724:1737	A single oral administration	1710:1737	A single oral administration of PGA and m-PGA	1710:1754	A single oral administration of PGA and m-PGA significantly reduced CAR-induced inflammatory signs (edema, inflammatory infiltrate, and hyperalgesia), and m-PGA also reduced the histological score.
31779692	5	74	theme	METHODS	600:606	arg1	toxicity					614:621	METHODS Acute toxicity	600:621	METHODS Acute toxicity of micronized PGA (m-PGA)	600:647	METHODS Acute toxicity of micronized PGA (m-PGA) was assessed in rats following OECD Guideline No.425.
31779692	15	75	theme	PGA	1742:1744	arg1	administration					1724:1737	A single oral administration	1710:1737	A single oral administration of PGA and m-PGA	1710:1754	A single oral administration of PGA and m-PGA significantly reduced CAR-induced inflammatory signs (edema, inflammatory infiltrate, and hyperalgesia), and m-PGA also reduced the histological score.
31779692	14	76	theme	LD50	1673:1676	arg1	values					1678:1683	LD50 values	1673:1683	LD50 values in excess of 2000 mg/kg	1673:1707	RESULTS Acute oral toxicity of m-PGA resulted in LD50 values in excess of 2000 mg/kg.
31779692	4	77	theme	N-palmitoyl-D-glucosamine	527:551	arg1	effectiveness					497:509	effectiveness	497:509	effectiveness	497:509	Here we investigated the safety and effectiveness of the ALIAmide N-palmitoyl-D-glucosamine (PGA) in inflammation and osteoarthritis pain.
31779692	4	77	theme	N-palmitoyl-D-glucosamine	527:551	arg1	safety					486:491	safety	486:491	safety	486:491	Here we investigated the safety and effectiveness of the ALIAmide N-palmitoyl-D-glucosamine (PGA) in inflammation and osteoarthritis pain.
31779692	0	78	theme	palmitoylglucosamine	68:87	arg1	formulation					40:50	a new micronized formulation	23:50	a new micronized formulation of the ALIAmide palmitoylglucosamine	23:87	Safety and efficacy of a new micronized formulation of the ALIAmide palmitoylglucosamine in preclinical models of inflammation and osteoarthritis pain.
31779692	4	79	dep	inflammation	562:573	arg1	pain					594:597	pain	594:597	pain	594:597	Here we investigated the safety and effectiveness of the ALIAmide N-palmitoyl-D-glucosamine (PGA) in inflammation and osteoarthritis pain.
31779692	15	80	dep	signs	1803:1807	arg1	edema					1810:1814	edema	1810:1814	edema	1810:1814	A single oral administration of PGA and m-PGA significantly reduced CAR-induced inflammatory signs (edema, inflammatory infiltrate, and hyperalgesia), and m-PGA also reduced the histological score.
31779692	15	80	dep	signs	1803:1807	arg1	infiltrate					1830:1839	inflammatory infiltrate	1817:1839	inflammatory infiltrate	1817:1839	A single oral administration of PGA and m-PGA significantly reduced CAR-induced inflammatory signs (edema, inflammatory infiltrate, and hyperalgesia), and m-PGA also reduced the histological score.
31779692	15	80	dep	signs	1803:1807	arg1	hyperalgesia					1846:1857	hyperalgesia	1846:1857	hyperalgesia	1846:1857	A single oral administration of PGA and m-PGA significantly reduced CAR-induced inflammatory signs (edema, inflammatory infiltrate, and hyperalgesia), and m-PGA also reduced the histological score.
31779692	15	80	dep	signs	1803:1807	arg1	signs					1803:1807	CAR-induced inflammatory signs	1778:1807	CAR-induced inflammatory signs (edema, inflammatory infiltrate, and hyperalgesia)	1778:1858	A single oral administration of PGA and m-PGA significantly reduced CAR-induced inflammatory signs (edema, inflammatory infiltrate, and hyperalgesia), and m-PGA also reduced the histological score.
31779692	11	81	theme	mast	1324:1327	arg1	cells					1329:1333	articular mast cells	1314:1333	articular mast cells	1314:1333	Joint histological and radiographic damage was scored, articular mast cells were counted, and macrophages were immunohistochemically investigated.
31779692	6	82	dep	PGA	703:705	arg1	30 mg/kg					718:725	30 mg/kg	718:725	30 mg/kg	718:725	PGA and m-PGA (30 mg/kg and 100 mg/kg) were orally administered to carrageenan (CAR)-injected rats.
31779692	6	82	dep	PGA	703:705	arg1	100 mg/kg					731:739	100 mg/kg	731:739	100 mg/kg	731:739	PGA and m-PGA (30 mg/kg and 100 mg/kg) were orally administered to carrageenan (CAR)-injected rats.
31779692	15	83	theme	single	1712:1717	arg1	administration					1724:1737	A single oral administration	1710:1737	A single oral administration of PGA and m-PGA	1710:1754	A single oral administration of PGA and m-PGA significantly reduced CAR-induced inflammatory signs (edema, inflammatory infiltrate, and hyperalgesia), and m-PGA also reduced the histological score.
31779692	0	84	theme	preclinical	92:102	arg1	models					104:109	preclinical models	92:109	preclinical models of inflammation	92:125	Safety and efficacy of a new micronized formulation of the ALIAmide palmitoylglucosamine in preclinical models of inflammation and osteoarthritis pain.
31779692	16	85	theme	mechanical	1977:1986	arg1	allodynia					1988:1996	MIA-induced mechanical allodynia	1965:1996	MIA-induced mechanical allodynia	1965:1996	Micronized PGA resulted in a superior activity to PGA on MIA-induced mechanical allodynia, locomotor disability, and histologic and radiographic damage.
31779692	8	86	theme	thermal	864:870	arg1	hyperalgesia					872:883	thermal hyperalgesia	864:883	thermal hyperalgesia	864:883	Paw edema and thermal hyperalgesia were measured up to 6 h post-injection, when also myeloperoxidase activity and histological inflammation score were assessed.
31779692	16	87	theme	radiographic	2040:2051	arg1	damage					2053:2058	histologic and radiographic damage	2025:2058	damage	2053:2058	Micronized PGA resulted in a superior activity to PGA on MIA-induced mechanical allodynia, locomotor disability, and histologic and radiographic damage.
31779692	20	88	theme	osteoarthritis	2585:2598	arg1	management					2571:2580	the management	2567:2580	the management of osteoarthritis	2567:2598	Given these results, m-PGA could be considered a valuable option in the management of osteoarthritis.
31779692	14	89	theme	oral	1638:1641	arg1	toxicity					1643:1650	Acute oral toxicity	1632:1650	Acute oral toxicity of m-PGA	1632:1659	RESULTS Acute oral toxicity of m-PGA resulted in LD50 values in excess of 2000 mg/kg.
31779692	9	90	theme	sodium	1058:1063	arg1	MIA					1082:1084	MIA	1082:1084	MIA	1082:1084	Rats subjected to intra-articular injection of sodium monoiodoacetate (MIA) were treated three times per week for 21 days with PGA or m-PGA (30 mg/kg).
31779692	9	90	theme	sodium	1058:1063	arg1	monoiodoacetate					1065:1079	sodium monoiodoacetate	1058:1079	sodium monoiodoacetate (MIA)	1058:1085	Rats subjected to intra-articular injection of sodium monoiodoacetate (MIA) were treated three times per week for 21 days with PGA or m-PGA (30 mg/kg).
31779692	3	91	theme	mast	412:415	arg1	cells					417:421	mast cells	412:421	mast cells	412:421	ALIAmides down-modulate mast cells and more generally hyperactive cells.
31779692	15	92	theme	inflammatory	1790:1801	arg1	edema					1810:1814	edema	1810:1814	edema	1810:1814	A single oral administration of PGA and m-PGA significantly reduced CAR-induced inflammatory signs (edema, inflammatory infiltrate, and hyperalgesia), and m-PGA also reduced the histological score.
31779692	15	92	theme	inflammatory	1790:1801	arg1	infiltrate					1830:1839	inflammatory infiltrate	1817:1839	inflammatory infiltrate	1817:1839	A single oral administration of PGA and m-PGA significantly reduced CAR-induced inflammatory signs (edema, inflammatory infiltrate, and hyperalgesia), and m-PGA also reduced the histological score.
31779692	15	92	theme	inflammatory	1790:1801	arg1	signs					1803:1807	CAR-induced inflammatory signs	1778:1807	CAR-induced inflammatory signs (edema, inflammatory infiltrate, and hyperalgesia)	1778:1858	A single oral administration of PGA and m-PGA significantly reduced CAR-induced inflammatory signs (edema, inflammatory infiltrate, and hyperalgesia), and m-PGA also reduced the histological score.
31779692	15	92	theme	inflammatory	1790:1801	arg1	hyperalgesia					1846:1857	hyperalgesia	1846:1857	hyperalgesia	1846:1857	A single oral administration of PGA and m-PGA significantly reduced CAR-induced inflammatory signs (edema, inflammatory infiltrate, and hyperalgesia), and m-PGA also reduced the histological score.
31779692	19	93	theme	PGA	2451:2453	arg1	activity					2439:2446	the activity	2435:2446	the activity of PGA	2435:2453	Moreover, it proved that particle size reduction greatly enhances the activity of PGA, particularly on joint pain and disability.
31779692	5	94	theme	micronized	626:635	arg1	m-PGA					642:646	m-PGA	642:646	m-PGA	642:646	METHODS Acute toxicity of micronized PGA (m-PGA) was assessed in rats following OECD Guideline No.425.
31779692	5	94	theme	micronized	626:635	arg1	PGA					637:639	micronized PGA	626:639	micronized PGA (m-PGA)	626:647	METHODS Acute toxicity of micronized PGA (m-PGA) was assessed in rats following OECD Guideline No.425.
31779692	8	95	theme	6 h	905:907	arg1	post-injection					909:922	6 h post-injection	905:922	6 h post-injection	905:922	Paw edema and thermal hyperalgesia were measured up to 6 h post-injection, when also myeloperoxidase activity and histological inflammation score were assessed.
31779692	11	96	theme	histological	1265:1276	arg1	damage					1295:1300	Joint histological and radiographic damage	1259:1300	Joint histological and radiographic damage	1259:1300	Joint histological and radiographic damage was scored, articular mast cells were counted, and macrophages were immunohistochemically investigated.
31779692	0	97	theme	new	25:27	arg1	formulation					40:50	a new micronized formulation	23:50	a new micronized formulation of the ALIAmide palmitoylglucosamine	23:87	Safety and efficacy of a new micronized formulation of the ALIAmide palmitoylglucosamine in preclinical models of inflammation and osteoarthritis pain.
31779692	12	98	theme	ELISA	1516:1520	arg1	kits					1522:1525	commercial colorimetric ELISA kits	1492:1525	commercial colorimetric ELISA kits	1492:1525	Levels of TNF-α, IL-1β, NGF, and MMP-1, MMP-3, and MMP-9 were measured in serum using commercial colorimetric ELISA kits.
31779692	15	99	theme	histological	1888:1899	arg1	score					1901:1905	the histological score	1884:1905	the histological score	1884:1905	A single oral administration of PGA and m-PGA significantly reduced CAR-induced inflammatory signs (edema, inflammatory infiltrate, and hyperalgesia), and m-PGA also reduced the histological score.
31779692	7	100	used	used	831:834	arg2	0.1 mg/kg					817:825	Dexamethasone 0.1 mg/kg	803:825	Dexamethasone 0.1 mg/kg	803:825	Dexamethasone 0.1 mg/kg was used as reference.
31779692	7	100	used	used	831:834	arg2	reference					839:847	reference	839:847	reference	839:847	Dexamethasone 0.1 mg/kg was used as reference.
31779692	17	101	theme	investigated	2128:2139	arg1	markers					2141:2147	the investigated markers	2124:2147	the investigated markers	2124:2147	The MIA-induced increase in mast cell count and serum level of the investigated markers was also counteracted by PGA and to a significantly greater extent by m-PGA.
31779692	0	102	theme	formulation	40:50	arg1	osteoarthritis					131:144	osteoarthritis	131:144	osteoarthritis	131:144	Safety and efficacy of a new micronized formulation of the ALIAmide palmitoylglucosamine in preclinical models of inflammation and osteoarthritis pain.
31779692	0	102	theme	formulation	40:50	arg1	efficacy					11:18	efficacy	11:18	efficacy	11:18	Safety and efficacy of a new micronized formulation of the ALIAmide palmitoylglucosamine in preclinical models of inflammation and osteoarthritis pain.
31779692	0	102	theme	formulation	40:50	arg1	Safety					0:5	Safety	0:5	Safety	0:5	Safety and efficacy of a new micronized formulation of the ALIAmide palmitoylglucosamine in preclinical models of inflammation and osteoarthritis pain.
31779692	19	103	theme	joint	2472:2476	arg1	pain					2478:2481	joint pain	2472:2481	joint pain	2472:2481	Moreover, it proved that particle size reduction greatly enhances the activity of PGA, particularly on joint pain and disability.
31779692	16	104	theme	superior	1937:1944	arg1	activity					1946:1953	a superior activity	1935:1953	a superior activity to PGA on MIA-induced mechanical allodynia, locomotor disability, and histologic and radiographic damage	1935:2058	Micronized PGA resulted in a superior activity to PGA on MIA-induced mechanical allodynia, locomotor disability, and histologic and radiographic damage.
31779692	20	105	theme	valuable	2548:2555	arg1	option					2557:2562	a valuable option	2546:2562	a valuable option	2546:2562	Given these results, m-PGA could be considered a valuable option in the management of osteoarthritis.
31779692	11	106	theme	radiographic	1282:1293	arg1	damage					1295:1300	Joint histological and radiographic damage	1259:1300	Joint histological and radiographic damage	1259:1300	Joint histological and radiographic damage was scored, articular mast cells were counted, and macrophages were immunohistochemically investigated.
31779692	12	107	theme	commercial	1492:1501	arg1	kits					1522:1525	commercial colorimetric ELISA kits	1492:1525	commercial colorimetric ELISA kits	1492:1525	Levels of TNF-α, IL-1β, NGF, and MMP-1, MMP-3, and MMP-9 were measured in serum using commercial colorimetric ELISA kits.
31779692	8	108	theme	histological	964:975	arg1	score					990:994	histological inflammation score	964:994	histological inflammation score	964:994	Paw edema and thermal hyperalgesia were measured up to 6 h post-injection, when also myeloperoxidase activity and histological inflammation score were assessed.
31779692	18	109	theme	study	2265:2269	arg1	results					2242:2248	The results	2238:2248	CONCLUSIONS The results of the present study	2226:2269	CONCLUSIONS The results of the present study showed that PGA is endorsed with anti-inflammatory, pain-relieving, and joint-protective effects.
31779692	2	110	theme	detrimental	369:379	arg1	triad					381:385	this detrimental triad	364:385	this detrimental triad	364:385	Joint mast cells are considered to play a key role in orchestrating this detrimental triad.
31779692	10	111	theme	motor	1188:1192	arg1	function					1194:1201	motor function	1188:1201	motor function	1188:1201	Mechanical allodynia and motor function were evaluated at different post-injection time points.
30808878	4	0	theme	stereomicroscopic	845:861	arg1	analysis					876:883	the classical stereomicroscopic histological analysis	831:883	the classical stereomicroscopic histological analysis based on dentin color and translucency	831:922	Histologically, microradiographic analysis with contrast solution (MRC) in dentine revealed a decreased proportion of sclerotic dentine and an increased proportion of deep dentine demineralization compared to the classical stereomicroscopic histological analysis based on dentin color and translucency.
30808878	1	1	theme	enamel	133:138	arg1	NEC					148:150	NEC	148:150	NEC	148:150	It is believed that penetration of dentinal fluid into natural enamel caries (NEC) is negligible because of the barrier created by underlying sclerotic dentine, but there are conflicting evidences on whether dentine subjacent to NEC is sclerotic or demineralized.
30808878	1	1	theme	enamel	133:138	arg1	caries					140:145	natural enamel caries	125:145	natural enamel caries (NEC)	125:151	It is believed that penetration of dentinal fluid into natural enamel caries (NEC) is negligible because of the barrier created by underlying sclerotic dentine, but there are conflicting evidences on whether dentine subjacent to NEC is sclerotic or demineralized.
30808878	1	2	theme	sclerotic	212:220	arg1	dentine					222:228	underlying sclerotic dentine	201:228	underlying sclerotic dentine	201:228	It is believed that penetration of dentinal fluid into natural enamel caries (NEC) is negligible because of the barrier created by underlying sclerotic dentine, but there are conflicting evidences on whether dentine subjacent to NEC is sclerotic or demineralized.
30808878	2	3	theme	fluid	452:456	arg1	composition					463:473	composition	463:473	composition	463:473	This study aimed at investigating the relationship between NEC, subjacent dentine reactions, modification of dentinal fluid, and composition of cariogenic biofilm formed on the NEC surface.
30808878	2	3	theme	fluid	452:456	arg1	modification					427:438	modification	427:438	modification of dentinal fluid	427:456	This study aimed at investigating the relationship between NEC, subjacent dentine reactions, modification of dentinal fluid, and composition of cariogenic biofilm formed on the NEC surface.
30808878	2	3	theme	fluid	452:456	arg1	reactions					416:424	subjacent dentine reactions	398:424	subjacent dentine reactions	398:424	This study aimed at investigating the relationship between NEC, subjacent dentine reactions, modification of dentinal fluid, and composition of cariogenic biofilm formed on the NEC surface.
30808878	2	3	theme	fluid	452:456	arg1	NEC					393:395	NEC	393:395	NEC	393:395	This study aimed at investigating the relationship between NEC, subjacent dentine reactions, modification of dentinal fluid, and composition of cariogenic biofilm formed on the NEC surface.
30808878	4	4	theme	classical	835:843	arg1	analysis					876:883	the classical stereomicroscopic histological analysis	831:883	the classical stereomicroscopic histological analysis based on dentin color and translucency	831:922	Histologically, microradiographic analysis with contrast solution (MRC) in dentine revealed a decreased proportion of sclerotic dentine and an increased proportion of deep dentine demineralization compared to the classical stereomicroscopic histological analysis based on dentin color and translucency.
30808878	5	5	theme	3D	972:973	arg1	analysis					992:999	Real-time MRC and 3D optical profilometry, and 3D microtomographic analysis	925:999	analysis	992:999	Real-time MRC and 3D optical profilometry, and 3D microtomographic analysis evidenced a facilitated transport of modified dentinal fluid towards PNEC lesions.
30808878	4	6	theme	dentine	750:756	arg1	proportion					726:735	a decreased proportion	714:735	a decreased proportion of sclerotic dentine	714:756	Histologically, microradiographic analysis with contrast solution (MRC) in dentine revealed a decreased proportion of sclerotic dentine and an increased proportion of deep dentine demineralization compared to the classical stereomicroscopic histological analysis based on dentin color and translucency.
30808878	4	6	theme	dentine	750:756	arg1	proportion					775:784	an increased proportion	762:784	an increased proportion of deep dentine demineralization	762:817	Histologically, microradiographic analysis with contrast solution (MRC) in dentine revealed a decreased proportion of sclerotic dentine and an increased proportion of deep dentine demineralization compared to the classical stereomicroscopic histological analysis based on dentin color and translucency.
30808878	6	7	theme	polysaccharides	1195:1209	arg1	amounts					1155:1161	lower amounts	1149:1161	lower amounts of insoluble and soluble matrix polysaccharides	1149:1209	Cariogenic biofilm formed in vitro on the PNEC surface presented lower amounts of insoluble and soluble matrix polysaccharides when 2% chlorexidine was inserted in the pulp chamber.
30808878	6	7	theme	polysaccharides	1195:1209	arg1	polysaccharides					1195:1209	insoluble and soluble matrix polysaccharides	1166:1209	insoluble and soluble matrix polysaccharides	1166:1209	Cariogenic biofilm formed in vitro on the PNEC surface presented lower amounts of insoluble and soluble matrix polysaccharides when 2% chlorexidine was inserted in the pulp chamber.
30808878	5	8	theme	optical	946:952	arg1	profilometry					954:965	Real-time MRC and 3D optical profilometry, and 3D microtomographic analysis	925:999	profilometry	954:965	Real-time MRC and 3D optical profilometry, and 3D microtomographic analysis evidenced a facilitated transport of modified dentinal fluid towards PNEC lesions.
30808878	2	9	theme	biofilm	489:495	arg1	composition					463:473	composition	463:473	composition	463:473	This study aimed at investigating the relationship between NEC, subjacent dentine reactions, modification of dentinal fluid, and composition of cariogenic biofilm formed on the NEC surface.
30808878	2	9	theme	biofilm	489:495	arg1	modification					427:438	modification	427:438	modification of dentinal fluid	427:456	This study aimed at investigating the relationship between NEC, subjacent dentine reactions, modification of dentinal fluid, and composition of cariogenic biofilm formed on the NEC surface.
30808878	2	9	theme	biofilm	489:495	arg1	reactions					416:424	subjacent dentine reactions	398:424	subjacent dentine reactions	398:424	This study aimed at investigating the relationship between NEC, subjacent dentine reactions, modification of dentinal fluid, and composition of cariogenic biofilm formed on the NEC surface.
30808878	2	9	theme	biofilm	489:495	arg1	NEC					393:395	NEC	393:395	NEC	393:395	This study aimed at investigating the relationship between NEC, subjacent dentine reactions, modification of dentinal fluid, and composition of cariogenic biofilm formed on the NEC surface.
30808878	7	10	theme	biofilm	1463:1469	arg1	composition					1444:1454	the composition	1440:1454	the composition of the biofilm formed on the PNEC surface	1440:1496	In conclusion, this study evidenced that dentine subjacent to PNEC is mostly demineralized, providing facilitated pathway for dentinal fluid to penetrate into PNEC and alter the composition of the biofilm formed on the PNEC surface.
30808878	4	11	theme	histological	863:874	arg1	analysis					876:883	the classical stereomicroscopic histological analysis	831:883	the classical stereomicroscopic histological analysis based on dentin color and translucency	831:922	Histologically, microradiographic analysis with contrast solution (MRC) in dentine revealed a decreased proportion of sclerotic dentine and an increased proportion of deep dentine demineralization compared to the classical stereomicroscopic histological analysis based on dentin color and translucency.
30808878	4	12	theme	contrast	670:677	arg1	MRC					689:691	MRC	689:691	MRC	689:691	Histologically, microradiographic analysis with contrast solution (MRC) in dentine revealed a decreased proportion of sclerotic dentine and an increased proportion of deep dentine demineralization compared to the classical stereomicroscopic histological analysis based on dentin color and translucency.
30808878	4	12	theme	contrast	670:677	arg1	solution					679:686	contrast solution	670:686	contrast solution (MRC) in dentine	670:703	Histologically, microradiographic analysis with contrast solution (MRC) in dentine revealed a decreased proportion of sclerotic dentine and an increased proportion of deep dentine demineralization compared to the classical stereomicroscopic histological analysis based on dentin color and translucency.
30808878	5	13	theme	Real-time	925:933	arg1	MRC					935:937	Real-time MRC and 3D optical profilometry, and 3D microtomographic analysis	925:999	MRC	935:937	Real-time MRC and 3D optical profilometry, and 3D microtomographic analysis evidenced a facilitated transport of modified dentinal fluid towards PNEC lesions.
30808878	2	14	theme	cariogenic	478:487	arg1	biofilm					489:495	cariogenic biofilm	478:495	cariogenic biofilm formed on the NEC surface	478:521	This study aimed at investigating the relationship between NEC, subjacent dentine reactions, modification of dentinal fluid, and composition of cariogenic biofilm formed on the NEC surface.
30808878	7	15	theme	dentinal	1392:1399	arg1	fluid					1401:1405	dentinal fluid	1392:1405	dentinal fluid	1392:1405	In conclusion, this study evidenced that dentine subjacent to PNEC is mostly demineralized, providing facilitated pathway for dentinal fluid to penetrate into PNEC and alter the composition of the biofilm formed on the PNEC surface.
30808878	4	16	theme	sclerotic	740:748	arg1	dentine					750:756	sclerotic dentine	740:756	sclerotic dentine	740:756	Histologically, microradiographic analysis with contrast solution (MRC) in dentine revealed a decreased proportion of sclerotic dentine and an increased proportion of deep dentine demineralization compared to the classical stereomicroscopic histological analysis based on dentin color and translucency.
30808878	6	17	theme	%	1217:1217	arg1	chlorexidine					1219:1230	2% chlorexidine	1216:1230	2% chlorexidine	1216:1230	Cariogenic biofilm formed in vitro on the PNEC surface presented lower amounts of insoluble and soluble matrix polysaccharides when 2% chlorexidine was inserted in the pulp chamber.
30808878	4	18	with	analysis	656:663	arg1	MRC					689:691	MRC	689:691	MRC	689:691	Histologically, microradiographic analysis with contrast solution (MRC) in dentine revealed a decreased proportion of sclerotic dentine and an increased proportion of deep dentine demineralization compared to the classical stereomicroscopic histological analysis based on dentin color and translucency.
30808878	4	18	with	analysis	656:663	arg1	solution					679:686	contrast solution	670:686	contrast solution (MRC) in dentine	670:703	Histologically, microradiographic analysis with contrast solution (MRC) in dentine revealed a decreased proportion of sclerotic dentine and an increased proportion of deep dentine demineralization compared to the classical stereomicroscopic histological analysis based on dentin color and translucency.
30808878	4	19	theme	demineralization	802:817	arg1	proportion					726:735	a decreased proportion	714:735	a decreased proportion of sclerotic dentine	714:756	Histologically, microradiographic analysis with contrast solution (MRC) in dentine revealed a decreased proportion of sclerotic dentine and an increased proportion of deep dentine demineralization compared to the classical stereomicroscopic histological analysis based on dentin color and translucency.
30808878	4	19	theme	demineralization	802:817	arg1	proportion					775:784	an increased proportion	762:784	an increased proportion of deep dentine demineralization	762:817	Histologically, microradiographic analysis with contrast solution (MRC) in dentine revealed a decreased proportion of sclerotic dentine and an increased proportion of deep dentine demineralization compared to the classical stereomicroscopic histological analysis based on dentin color and translucency.
30808878	0	20	theme	Natural	0:6	arg1	caries					15:20	Natural enamel caries	0:20	Natural enamel caries	0:20	Natural enamel caries, dentine reactions, dentinal fluid and biofilm.
30808878	6	21	theme	matrix	1188:1193	arg1	polysaccharides					1195:1209	insoluble and soluble matrix polysaccharides	1166:1209	insoluble and soluble matrix polysaccharides	1166:1209	Cariogenic biofilm formed in vitro on the PNEC surface presented lower amounts of insoluble and soluble matrix polysaccharides when 2% chlorexidine was inserted in the pulp chamber.
30808878	0	22	theme	enamel	8:13	arg1	caries					15:20	Natural enamel caries	0:20	Natural enamel caries	0:20	Natural enamel caries, dentine reactions, dentinal fluid and biofilm.
30808878	6	23	theme	insoluble	1166:1174	arg1	polysaccharides					1195:1209	insoluble and soluble matrix polysaccharides	1166:1209	insoluble and soluble matrix polysaccharides	1166:1209	Cariogenic biofilm formed in vitro on the PNEC surface presented lower amounts of insoluble and soluble matrix polysaccharides when 2% chlorexidine was inserted in the pulp chamber.
30808878	3	24	theme	NEC	533:535	arg1	lesions					544:550	Proximal NEC (PNEC) lesions	524:550	Proximal NEC (PNEC) lesions of human permanent posterior teeth	524:585	Proximal NEC (PNEC) lesions of human permanent posterior teeth were included in five experiments.
30808878	2	25	theme	dentine	408:414	arg1	reactions					416:424	subjacent dentine reactions	398:424	subjacent dentine reactions	398:424	This study aimed at investigating the relationship between NEC, subjacent dentine reactions, modification of dentinal fluid, and composition of cariogenic biofilm formed on the NEC surface.
30808878	6	26	theme	2	1216:1216	arg1	%					1217:1217	%	1217:1217	%	1217:1217	Cariogenic biofilm formed in vitro on the PNEC surface presented lower amounts of insoluble and soluble matrix polysaccharides when 2% chlorexidine was inserted in the pulp chamber.
30808878	6	27	theme	pulp	1252:1255	arg1	chamber					1257:1263	the pulp chamber	1248:1263	the pulp chamber	1248:1263	Cariogenic biofilm formed in vitro on the PNEC surface presented lower amounts of insoluble and soluble matrix polysaccharides when 2% chlorexidine was inserted in the pulp chamber.
30808878	2	28	theme	subjacent	398:406	arg1	reactions					416:424	subjacent dentine reactions	398:424	subjacent dentine reactions	398:424	This study aimed at investigating the relationship between NEC, subjacent dentine reactions, modification of dentinal fluid, and composition of cariogenic biofilm formed on the NEC surface.
30808878	4	29	theme	increased	765:773	arg1	proportion					775:784	an increased proportion	762:784	an increased proportion of deep dentine demineralization	762:817	Histologically, microradiographic analysis with contrast solution (MRC) in dentine revealed a decreased proportion of sclerotic dentine and an increased proportion of deep dentine demineralization compared to the classical stereomicroscopic histological analysis based on dentin color and translucency.
30808878	5	30	theme	modified	1038:1045	arg1	fluid					1056:1060	modified dentinal fluid	1038:1060	modified dentinal fluid towards PNEC lesions	1038:1081	Real-time MRC and 3D optical profilometry, and 3D microtomographic analysis evidenced a facilitated transport of modified dentinal fluid towards PNEC lesions.
30808878	5	31	theme	3D	943:944	arg1	profilometry					954:965	Real-time MRC and 3D optical profilometry, and 3D microtomographic analysis	925:999	profilometry	954:965	Real-time MRC and 3D optical profilometry, and 3D microtomographic analysis evidenced a facilitated transport of modified dentinal fluid towards PNEC lesions.
30808878	6	32	theme	Cariogenic	1084:1093	arg1	biofilm					1095:1101	Cariogenic biofilm	1084:1101	Cariogenic biofilm formed in vitro on the PNEC surface	1084:1137	Cariogenic biofilm formed in vitro on the PNEC surface presented lower amounts of insoluble and soluble matrix polysaccharides when 2% chlorexidine was inserted in the pulp chamber.
30808878	7	33	theme	dentine	1307:1313	arg1	subjacent					1315:1323	dentine subjacent	1307:1323	dentine subjacent to PNEC	1307:1331	In conclusion, this study evidenced that dentine subjacent to PNEC is mostly demineralized, providing facilitated pathway for dentinal fluid to penetrate into PNEC and alter the composition of the biofilm formed on the PNEC surface.
30808878	1	34	theme	conflicting	245:255	arg1	evidences					257:265	conflicting evidences	245:265	conflicting evidences	245:265	It is believed that penetration of dentinal fluid into natural enamel caries (NEC) is negligible because of the barrier created by underlying sclerotic dentine, but there are conflicting evidences on whether dentine subjacent to NEC is sclerotic or demineralized.
30808878	2	35	theme	NEC	511:513	arg1	surface					515:521	the NEC surface	507:521	the NEC surface	507:521	This study aimed at investigating the relationship between NEC, subjacent dentine reactions, modification of dentinal fluid, and composition of cariogenic biofilm formed on the NEC surface.
30808878	3	36	theme	Proximal	524:531	arg1	PNEC					538:541	PNEC	538:541	PNEC	538:541	Proximal NEC (PNEC) lesions of human permanent posterior teeth were included in five experiments.
30808878	3	36	theme	Proximal	524:531	arg1	NEC					533:535	Proximal NEC	524:535	Proximal NEC (PNEC) lesions of human permanent posterior teeth	524:585	Proximal NEC (PNEC) lesions of human permanent posterior teeth were included in five experiments.
30808878	7	37	theme	facilitated	1368:1378	arg1	pathway					1380:1386	facilitated pathway	1368:1386	facilitated pathway for dentinal fluid	1368:1405	In conclusion, this study evidenced that dentine subjacent to PNEC is mostly demineralized, providing facilitated pathway for dentinal fluid to penetrate into PNEC and alter the composition of the biofilm formed on the PNEC surface.
30808878	2	38	theme	dentinal	443:450	arg1	fluid					452:456	dentinal fluid	443:456	dentinal fluid	443:456	This study aimed at investigating the relationship between NEC, subjacent dentine reactions, modification of dentinal fluid, and composition of cariogenic biofilm formed on the NEC surface.
30808878	5	39	theme	facilitated	1013:1023	arg1	transport					1025:1033	a facilitated transport	1011:1033	a facilitated transport of modified dentinal fluid towards PNEC lesions	1011:1081	Real-time MRC and 3D optical profilometry, and 3D microtomographic analysis evidenced a facilitated transport of modified dentinal fluid towards PNEC lesions.
30808878	0	40	theme	dentine	23:29	arg1	reactions					31:39	dentine reactions	23:39	dentine reactions	23:39	Natural enamel caries, dentine reactions, dentinal fluid and biofilm.
30808878	4	41	theme	decreased	716:724	arg1	proportion					726:735	a decreased proportion	714:735	a decreased proportion of sclerotic dentine	714:756	Histologically, microradiographic analysis with contrast solution (MRC) in dentine revealed a decreased proportion of sclerotic dentine and an increased proportion of deep dentine demineralization compared to the classical stereomicroscopic histological analysis based on dentin color and translucency.
30808878	0	42	theme	dentinal	42:49	arg1	fluid					51:55	dentinal fluid	42:55	dentinal fluid	42:55	Natural enamel caries, dentine reactions, dentinal fluid and biofilm.
30808878	4	43	theme	dentine	794:800	arg1	demineralization					802:817	deep dentine demineralization	789:817	deep dentine demineralization	789:817	Histologically, microradiographic analysis with contrast solution (MRC) in dentine revealed a decreased proportion of sclerotic dentine and an increased proportion of deep dentine demineralization compared to the classical stereomicroscopic histological analysis based on dentin color and translucency.
30808878	1	44	theme	dentine	278:284	arg1	subjacent					286:294	dentine subjacent	278:294	dentine subjacent to NEC	278:301	It is believed that penetration of dentinal fluid into natural enamel caries (NEC) is negligible because of the barrier created by underlying sclerotic dentine, but there are conflicting evidences on whether dentine subjacent to NEC is sclerotic or demineralized.
30808878	7	45	theme	PNEC	1485:1488	arg1	surface					1490:1496	the PNEC surface	1481:1496	the PNEC surface	1481:1496	In conclusion, this study evidenced that dentine subjacent to PNEC is mostly demineralized, providing facilitated pathway for dentinal fluid to penetrate into PNEC and alter the composition of the biofilm formed on the PNEC surface.
30808878	5	46	theme	dentinal	1047:1054	arg1	fluid					1056:1060	modified dentinal fluid	1038:1060	modified dentinal fluid towards PNEC lesions	1038:1081	Real-time MRC and 3D optical profilometry, and 3D microtomographic analysis evidenced a facilitated transport of modified dentinal fluid towards PNEC lesions.
30808878	4	47	theme	deep	789:792	arg1	demineralization					802:817	deep dentine demineralization	789:817	deep dentine demineralization	789:817	Histologically, microradiographic analysis with contrast solution (MRC) in dentine revealed a decreased proportion of sclerotic dentine and an increased proportion of deep dentine demineralization compared to the classical stereomicroscopic histological analysis based on dentin color and translucency.
30808878	5	48	theme	fluid	1056:1060	arg1	transport					1025:1033	a facilitated transport	1011:1033	a facilitated transport of modified dentinal fluid towards PNEC lesions	1011:1081	Real-time MRC and 3D optical profilometry, and 3D microtomographic analysis evidenced a facilitated transport of modified dentinal fluid towards PNEC lesions.
30808878	3	49	theme	posterior	571:579	arg1	teeth					581:585	human permanent posterior teeth	555:585	human permanent posterior teeth	555:585	Proximal NEC (PNEC) lesions of human permanent posterior teeth were included in five experiments.
30808878	4	50	theme	microradiographic	638:654	arg1	analysis					656:663	microradiographic analysis	638:663	microradiographic analysis with contrast solution (MRC) in dentine	638:703	Histologically, microradiographic analysis with contrast solution (MRC) in dentine revealed a decreased proportion of sclerotic dentine and an increased proportion of deep dentine demineralization compared to the classical stereomicroscopic histological analysis based on dentin color and translucency.
30808878	1	51	theme	dentinal	105:112	arg1	fluid					114:118	dentinal fluid	105:118	dentinal fluid	105:118	It is believed that penetration of dentinal fluid into natural enamel caries (NEC) is negligible because of the barrier created by underlying sclerotic dentine, but there are conflicting evidences on whether dentine subjacent to NEC is sclerotic or demineralized.
30808878	4	52	theme	dentin	894:899	arg1	color					901:905	dentin color	894:905	dentin color	894:905	Histologically, microradiographic analysis with contrast solution (MRC) in dentine revealed a decreased proportion of sclerotic dentine and an increased proportion of deep dentine demineralization compared to the classical stereomicroscopic histological analysis based on dentin color and translucency.
30808878	3	53	theme	teeth	581:585	arg1	lesions					544:550	Proximal NEC (PNEC) lesions	524:550	Proximal NEC (PNEC) lesions of human permanent posterior teeth	524:585	Proximal NEC (PNEC) lesions of human permanent posterior teeth were included in five experiments.
30808878	6	54	theme	soluble	1180:1186	arg1	polysaccharides					1195:1209	insoluble and soluble matrix polysaccharides	1166:1209	insoluble and soluble matrix polysaccharides	1166:1209	Cariogenic biofilm formed in vitro on the PNEC surface presented lower amounts of insoluble and soluble matrix polysaccharides when 2% chlorexidine was inserted in the pulp chamber.
30808878	1	55	theme	fluid	114:118	arg1	penetration					90:100	penetration	90:100	penetration of dentinal fluid into natural enamel caries (NEC)	90:151	It is believed that penetration of dentinal fluid into natural enamel caries (NEC) is negligible because of the barrier created by underlying sclerotic dentine, but there are conflicting evidences on whether dentine subjacent to NEC is sclerotic or demineralized.
30808878	6	56	theme	PNEC	1126:1129	arg1	surface					1131:1137	the PNEC surface	1122:1137	the PNEC surface	1122:1137	Cariogenic biofilm formed in vitro on the PNEC surface presented lower amounts of insoluble and soluble matrix polysaccharides when 2% chlorexidine was inserted in the pulp chamber.
30808878	5	57	theme	PNEC	1070:1073	arg1	lesions					1075:1081	PNEC lesions	1070:1081	PNEC lesions	1070:1081	Real-time MRC and 3D optical profilometry, and 3D microtomographic analysis evidenced a facilitated transport of modified dentinal fluid towards PNEC lesions.
30808878	3	58	theme	human	555:559	arg1	teeth					581:585	human permanent posterior teeth	555:585	human permanent posterior teeth	555:585	Proximal NEC (PNEC) lesions of human permanent posterior teeth were included in five experiments.
30808878	4	59	from	solution	679:686	arg1	dentine					697:703	dentine	697:703	dentine	697:703	Histologically, microradiographic analysis with contrast solution (MRC) in dentine revealed a decreased proportion of sclerotic dentine and an increased proportion of deep dentine demineralization compared to the classical stereomicroscopic histological analysis based on dentin color and translucency.
30808878	5	60	theme	microtomographic	975:990	arg1	analysis					992:999	Real-time MRC and 3D optical profilometry, and 3D microtomographic analysis	925:999	analysis	992:999	Real-time MRC and 3D optical profilometry, and 3D microtomographic analysis evidenced a facilitated transport of modified dentinal fluid towards PNEC lesions.
30808878	6	61	theme	lower	1149:1153	arg1	amounts					1155:1161	lower amounts	1149:1161	lower amounts of insoluble and soluble matrix polysaccharides	1149:1209	Cariogenic biofilm formed in vitro on the PNEC surface presented lower amounts of insoluble and soluble matrix polysaccharides when 2% chlorexidine was inserted in the pulp chamber.
30808878	6	61	theme	lower	1149:1153	arg1	polysaccharides					1195:1209	insoluble and soluble matrix polysaccharides	1166:1209	insoluble and soluble matrix polysaccharides	1166:1209	Cariogenic biofilm formed in vitro on the PNEC surface presented lower amounts of insoluble and soluble matrix polysaccharides when 2% chlorexidine was inserted in the pulp chamber.
30808878	3	62	theme	permanent	561:569	arg1	teeth					581:585	human permanent posterior teeth	555:585	human permanent posterior teeth	555:585	Proximal NEC (PNEC) lesions of human permanent posterior teeth were included in five experiments.
30808878	1	63	theme	natural	125:131	arg1	NEC					148:150	NEC	148:150	NEC	148:150	It is believed that penetration of dentinal fluid into natural enamel caries (NEC) is negligible because of the barrier created by underlying sclerotic dentine, but there are conflicting evidences on whether dentine subjacent to NEC is sclerotic or demineralized.
30808878	1	63	theme	natural	125:131	arg1	caries					140:145	natural enamel caries	125:145	natural enamel caries (NEC)	125:151	It is believed that penetration of dentinal fluid into natural enamel caries (NEC) is negligible because of the barrier created by underlying sclerotic dentine, but there are conflicting evidences on whether dentine subjacent to NEC is sclerotic or demineralized.
30808878	1	64	theme	underlying	201:210	arg1	dentine					222:228	underlying sclerotic dentine	201:228	underlying sclerotic dentine	201:228	It is believed that penetration of dentinal fluid into natural enamel caries (NEC) is negligible because of the barrier created by underlying sclerotic dentine, but there are conflicting evidences on whether dentine subjacent to NEC is sclerotic or demineralized.
31626297	2	0	theme	seawater	462:469	arg1	brine					471:475	relic (late Pleistocene) seawater brine	437:475	relic (late Pleistocene) seawater brine	437:475	Near Utqiaġvik, Alaska, we sampled subzero brines (-6°C, 115-140 ppt) from cryopegs, i.e. unfrozen sediments within permafrost that contain relic (late Pleistocene) seawater brine, as well as nearby sea-ice brines to examine microbial community composition and diversity using 16S rRNA gene amplicon sequencing.
31626297	0	1	from	communities	22:32	arg1	ice					88:90	Arctic coastal sea ice	69:90	Arctic coastal sea ice	69:90	Distinctive microbial communities in subzero hypersaline brines from Arctic coastal sea ice and rarely sampled cryopegs.
31626297	0	1	from	communities	22:32	arg1	brines					57:62	subzero hypersaline brines	37:62	subzero hypersaline brines from Arctic coastal sea ice	37:90	Distinctive microbial communities in subzero hypersaline brines from Arctic coastal sea ice and rarely sampled cryopegs.
31626297	2	2	theme	rRNA	578:581	arg1	sequencing					597:606	16S rRNA gene amplicon sequencing	574:606	16S rRNA gene amplicon sequencing	574:606	Near Utqiaġvik, Alaska, we sampled subzero brines (-6°C, 115-140 ppt) from cryopegs, i.e. unfrozen sediments within permafrost that contain relic (late Pleistocene) seawater brine, as well as nearby sea-ice brines to examine microbial community composition and diversity using 16S rRNA gene amplicon sequencing.
31626297	4	3	theme	extracellular	906:918	arg1	polysaccharides					920:934	extracellular polysaccharides	906:934	extracellular polysaccharides	906:934	The cryopeg brines harbored surprisingly dense bacterial communities (up to 108 cells mL-1) and millimolar levels of dissolved and particulate organic matter, extracellular polysaccharides and ammonia.
31626297	0	4	theme	sea	84:86	arg1	ice					88:90	Arctic coastal sea ice	69:90	Arctic coastal sea ice	69:90	Distinctive microbial communities in subzero hypersaline brines from Arctic coastal sea ice and rarely sampled cryopegs.
31626297	3	5	theme	environmental	673:685	arg1	parameters					687:696	environmental parameters	673:696	environmental parameters	673:696	We also quantified the communities microscopically and assessed environmental parameters as possible determinants of community structure.
31626297	3	5	theme	environmental	673:685	arg1	determinants					710:721	possible determinants	701:721	possible determinants of community structure	701:744	We also quantified the communities microscopically and assessed environmental parameters as possible determinants of community structure.
31626297	2	6	theme	16S	574:576	arg1	sequencing					597:606	16S rRNA gene amplicon sequencing	574:606	16S rRNA gene amplicon sequencing	574:606	Near Utqiaġvik, Alaska, we sampled subzero brines (-6°C, 115-140 ppt) from cryopegs, i.e. unfrozen sediments within permafrost that contain relic (late Pleistocene) seawater brine, as well as nearby sea-ice brines to examine microbial community composition and diversity using 16S rRNA gene amplicon sequencing.
31626297	1	7	from	temperatures	165:176	arg1	environments					141:152	Hypersaline aqueous environments	121:152	Hypersaline aqueous environments at subzero temperatures	121:176	Hypersaline aqueous environments at subzero temperatures are known to be inhabited by microorganisms, yet information on community structure in subzero brines is very limited.
31626297	1	8	theme	subzero	265:271	arg1	brines					273:278	subzero brines	265:278	subzero brines	265:278	Hypersaline aqueous environments at subzero temperatures are known to be inhabited by microorganisms, yet information on community structure in subzero brines is very limited.
31626297	2	9	theme	nearby	489:494	arg1	brines					504:509	nearby sea-ice brines	489:509	nearby sea-ice brines	489:509	Near Utqiaġvik, Alaska, we sampled subzero brines (-6°C, 115-140 ppt) from cryopegs, i.e. unfrozen sediments within permafrost that contain relic (late Pleistocene) seawater brine, as well as nearby sea-ice brines to examine microbial community composition and diversity using 16S rRNA gene amplicon sequencing.
31626297	4	10	theme	organic	890:896	arg1	matter					898:903	particulate organic matter	878:903	particulate organic matter	878:903	The cryopeg brines harbored surprisingly dense bacterial communities (up to 108 cells mL-1) and millimolar levels of dissolved and particulate organic matter, extracellular polysaccharides and ammonia.
31626297	4	11	theme	polysaccharides	920:934	arg1	levels					854:859	millimolar levels	843:859	millimolar levels of dissolved and particulate organic matter, extracellular polysaccharides and ammonia	843:946	The cryopeg brines harbored surprisingly dense bacterial communities (up to 108 cells mL-1) and millimolar levels of dissolved and particulate organic matter, extracellular polysaccharides and ammonia.
31626297	4	11	theme	polysaccharides	920:934	arg1	communities					804:814	surprisingly dense bacterial communities	775:814	surprisingly dense bacterial communities (up to 108 cells mL-1)	775:837	The cryopeg brines harbored surprisingly dense bacterial communities (up to 108 cells mL-1) and millimolar levels of dissolved and particulate organic matter, extracellular polysaccharides and ammonia.
31626297	4	11	theme	polysaccharides	920:934	arg1	mL-1					833:836	up to 108 cells mL-1	817:836	up to 108 cells mL-1	817:836	The cryopeg brines harbored surprisingly dense bacterial communities (up to 108 cells mL-1) and millimolar levels of dissolved and particulate organic matter, extracellular polysaccharides and ammonia.
31626297	2	12	dep	-6°C	348:351	arg1	ppt					362:364	115-140 ppt	354:364	115-140 ppt	354:364	Near Utqiaġvik, Alaska, we sampled subzero brines (-6°C, 115-140 ppt) from cryopegs, i.e. unfrozen sediments within permafrost that contain relic (late Pleistocene) seawater brine, as well as nearby sea-ice brines to examine microbial community composition and diversity using 16S rRNA gene amplicon sequencing.
31626297	5	13	theme	sea	1241:1243	arg1	ice					1245:1247	sea ice	1241:1247	sea ice	1241:1247	Community composition and diversity differed between the two brine environments by alpha- and beta-diversity indices, with cryopeg brine communities appearing less diverse and dominated by one strain of the genus Marinobacter, also detected in other cold, hypersaline environments, including sea ice.
31626297	6	14	theme	subzero	1386:1392	arg1	brine					1394:1398	a subzero brine	1384:1398	a subzero brine	1384:1398	The higher density and trend toward lower diversity in the cryopeg communities suggest that long-term stability and other features of a subzero brine are more important selective forces than in situ temperature or salinity, even when the latter are extreme.
31626297	3	15	theme	community	726:734	arg1	structure					736:744	community structure	726:744	community structure	726:744	We also quantified the communities microscopically and assessed environmental parameters as possible determinants of community structure.
31626297	4	16	theme	particulate	878:888	arg1	matter					898:903	particulate organic matter	878:903	particulate organic matter	878:903	The cryopeg brines harbored surprisingly dense bacterial communities (up to 108 cells mL-1) and millimolar levels of dissolved and particulate organic matter, extracellular polysaccharides and ammonia.
31626297	3	17	theme	structure	736:744	arg1	parameters					687:696	environmental parameters	673:696	environmental parameters	673:696	We also quantified the communities microscopically and assessed environmental parameters as possible determinants of community structure.
31626297	3	17	theme	structure	736:744	arg1	determinants					710:721	possible determinants	701:721	possible determinants of community structure	701:744	We also quantified the communities microscopically and assessed environmental parameters as possible determinants of community structure.
31626297	4	18	theme	millimolar	843:852	arg1	levels					854:859	millimolar levels	843:859	millimolar levels of dissolved and particulate organic matter, extracellular polysaccharides and ammonia	843:946	The cryopeg brines harbored surprisingly dense bacterial communities (up to 108 cells mL-1) and millimolar levels of dissolved and particulate organic matter, extracellular polysaccharides and ammonia.
31626297	2	19	contain	contain	429:435	arg1	permafrost					413:422	permafrost	413:422	permafrost that contain relic (late Pleistocene) seawater brine	413:475	Near Utqiaġvik, Alaska, we sampled subzero brines (-6°C, 115-140 ppt) from cryopegs, i.e. unfrozen sediments within permafrost that contain relic (late Pleistocene) seawater brine, as well as nearby sea-ice brines to examine microbial community composition and diversity using 16S rRNA gene amplicon sequencing.
31626297	2	19	contain	contain	429:435	arg2	brine					471:475	relic (late Pleistocene) seawater brine	437:475	relic (late Pleistocene) seawater brine	437:475	Near Utqiaġvik, Alaska, we sampled subzero brines (-6°C, 115-140 ppt) from cryopegs, i.e. unfrozen sediments within permafrost that contain relic (late Pleistocene) seawater brine, as well as nearby sea-ice brines to examine microbial community composition and diversity using 16S rRNA gene amplicon sequencing.
31626297	4	20	theme	bacterial	794:802	arg1	communities					804:814	surprisingly dense bacterial communities	775:814	surprisingly dense bacterial communities (up to 108 cells mL-1)	775:837	The cryopeg brines harbored surprisingly dense bacterial communities (up to 108 cells mL-1) and millimolar levels of dissolved and particulate organic matter, extracellular polysaccharides and ammonia.
31626297	4	20	theme	bacterial	794:802	arg1	mL-1					833:836	up to 108 cells mL-1	817:836	up to 108 cells mL-1	817:836	The cryopeg brines harbored surprisingly dense bacterial communities (up to 108 cells mL-1) and millimolar levels of dissolved and particulate organic matter, extracellular polysaccharides and ammonia.
31626297	0	21	theme	sampled	103:109	arg1	cryopegs					111:118	rarely sampled cryopegs	96:118	rarely sampled cryopegs	96:118	Distinctive microbial communities in subzero hypersaline brines from Arctic coastal sea ice and rarely sampled cryopegs.
31626297	4	22	theme	dense	788:792	arg1	communities					804:814	surprisingly dense bacterial communities	775:814	surprisingly dense bacterial communities (up to 108 cells mL-1)	775:837	The cryopeg brines harbored surprisingly dense bacterial communities (up to 108 cells mL-1) and millimolar levels of dissolved and particulate organic matter, extracellular polysaccharides and ammonia.
31626297	4	22	theme	dense	788:792	arg1	mL-1					833:836	up to 108 cells mL-1	817:836	up to 108 cells mL-1	817:836	The cryopeg brines harbored surprisingly dense bacterial communities (up to 108 cells mL-1) and millimolar levels of dissolved and particulate organic matter, extracellular polysaccharides and ammonia.
31626297	0	23	theme	microbial	12:20	arg1	communities					22:32	Distinctive microbial communities	0:32	Distinctive microbial communities in subzero hypersaline brines from Arctic coastal sea ice	0:90	Distinctive microbial communities in subzero hypersaline brines from Arctic coastal sea ice and rarely sampled cryopegs.
31626297	6	24	theme	cryopeg	1309:1315	arg1	communities					1317:1327	the cryopeg communities	1305:1327	the cryopeg communities	1305:1327	The higher density and trend toward lower diversity in the cryopeg communities suggest that long-term stability and other features of a subzero brine are more important selective forces than in situ temperature or salinity, even when the latter are extreme.
31626297	2	25	theme	amplicon	588:595	arg1	sequencing					597:606	16S rRNA gene amplicon sequencing	574:606	16S rRNA gene amplicon sequencing	574:606	Near Utqiaġvik, Alaska, we sampled subzero brines (-6°C, 115-140 ppt) from cryopegs, i.e. unfrozen sediments within permafrost that contain relic (late Pleistocene) seawater brine, as well as nearby sea-ice brines to examine microbial community composition and diversity using 16S rRNA gene amplicon sequencing.
31626297	5	26	theme	cold	1199:1202	arg1	ice					1245:1247	sea ice	1241:1247	sea ice	1241:1247	Community composition and diversity differed between the two brine environments by alpha- and beta-diversity indices, with cryopeg brine communities appearing less diverse and dominated by one strain of the genus Marinobacter, also detected in other cold, hypersaline environments, including sea ice.
31626297	5	26	theme	cold	1199:1202	arg1	environments					1217:1228	other cold, hypersaline environments	1193:1228	other cold, hypersaline environments	1193:1228	Community composition and diversity differed between the two brine environments by alpha- and beta-diversity indices, with cryopeg brine communities appearing less diverse and dominated by one strain of the genus Marinobacter, also detected in other cold, hypersaline environments, including sea ice.
31626297	0	27	theme	Distinctive	0:10	arg1	communities					22:32	Distinctive microbial communities	0:32	Distinctive microbial communities in subzero hypersaline brines from Arctic coastal sea ice	0:90	Distinctive microbial communities in subzero hypersaline brines from Arctic coastal sea ice and rarely sampled cryopegs.
31626297	5	28	theme	brine	1010:1014	arg1	environments					1016:1027	the two brine environments	1002:1027	the two brine environments	1002:1027	Community composition and diversity differed between the two brine environments by alpha- and beta-diversity indices, with cryopeg brine communities appearing less diverse and dominated by one strain of the genus Marinobacter, also detected in other cold, hypersaline environments, including sea ice.
31626297	2	29	theme	gene	583:586	arg1	sequencing					597:606	16S rRNA gene amplicon sequencing	574:606	16S rRNA gene amplicon sequencing	574:606	Near Utqiaġvik, Alaska, we sampled subzero brines (-6°C, 115-140 ppt) from cryopegs, i.e. unfrozen sediments within permafrost that contain relic (late Pleistocene) seawater brine, as well as nearby sea-ice brines to examine microbial community composition and diversity using 16S rRNA gene amplicon sequencing.
31626297	5	30	theme	cryopeg	1072:1078	arg1	communities					1086:1096	cryopeg brine communities	1072:1096	cryopeg brine communities	1072:1096	Community composition and diversity differed between the two brine environments by alpha- and beta-diversity indices, with cryopeg brine communities appearing less diverse and dominated by one strain of the genus Marinobacter, also detected in other cold, hypersaline environments, including sea ice.
31626297	2	31	dep	relic	437:441	arg1	Pleistocene					449:459	late Pleistocene	444:459	late Pleistocene	444:459	Near Utqiaġvik, Alaska, we sampled subzero brines (-6°C, 115-140 ppt) from cryopegs, i.e. unfrozen sediments within permafrost that contain relic (late Pleistocene) seawater brine, as well as nearby sea-ice brines to examine microbial community composition and diversity using 16S rRNA gene amplicon sequencing.
31626297	4	32	theme	ammonia	940:946	arg1	levels					854:859	millimolar levels	843:859	millimolar levels of dissolved and particulate organic matter, extracellular polysaccharides and ammonia	843:946	The cryopeg brines harbored surprisingly dense bacterial communities (up to 108 cells mL-1) and millimolar levels of dissolved and particulate organic matter, extracellular polysaccharides and ammonia.
31626297	4	32	theme	ammonia	940:946	arg1	communities					804:814	surprisingly dense bacterial communities	775:814	surprisingly dense bacterial communities (up to 108 cells mL-1)	775:837	The cryopeg brines harbored surprisingly dense bacterial communities (up to 108 cells mL-1) and millimolar levels of dissolved and particulate organic matter, extracellular polysaccharides and ammonia.
31626297	4	32	theme	ammonia	940:946	arg1	mL-1					833:836	up to 108 cells mL-1	817:836	up to 108 cells mL-1	817:836	The cryopeg brines harbored surprisingly dense bacterial communities (up to 108 cells mL-1) and millimolar levels of dissolved and particulate organic matter, extracellular polysaccharides and ammonia.
31626297	4	33	theme	dissolved	864:872	arg1	levels					854:859	millimolar levels	843:859	millimolar levels of dissolved and particulate organic matter, extracellular polysaccharides and ammonia	843:946	The cryopeg brines harbored surprisingly dense bacterial communities (up to 108 cells mL-1) and millimolar levels of dissolved and particulate organic matter, extracellular polysaccharides and ammonia.
31626297	4	33	theme	dissolved	864:872	arg1	communities					804:814	surprisingly dense bacterial communities	775:814	surprisingly dense bacterial communities (up to 108 cells mL-1)	775:837	The cryopeg brines harbored surprisingly dense bacterial communities (up to 108 cells mL-1) and millimolar levels of dissolved and particulate organic matter, extracellular polysaccharides and ammonia.
31626297	4	33	theme	dissolved	864:872	arg1	mL-1					833:836	up to 108 cells mL-1	817:836	up to 108 cells mL-1	817:836	The cryopeg brines harbored surprisingly dense bacterial communities (up to 108 cells mL-1) and millimolar levels of dissolved and particulate organic matter, extracellular polysaccharides and ammonia.
31626297	6	34	theme	selective	1419:1427	arg1	forces					1429:1434	more important selective forces	1404:1434	more important selective forces	1404:1434	The higher density and trend toward lower diversity in the cryopeg communities suggest that long-term stability and other features of a subzero brine are more important selective forces than in situ temperature or salinity, even when the latter are extreme.
31626297	6	34	theme	selective	1419:1427	arg1	stability					1352:1360	long-term stability	1342:1360	long-term stability	1342:1360	The higher density and trend toward lower diversity in the cryopeg communities suggest that long-term stability and other features of a subzero brine are more important selective forces than in situ temperature or salinity, even when the latter are extreme.
31626297	6	34	theme	selective	1419:1427	arg1	features					1372:1379	other features	1366:1379	other features of a subzero brine	1366:1398	The higher density and trend toward lower diversity in the cryopeg communities suggest that long-term stability and other features of a subzero brine are more important selective forces than in situ temperature or salinity, even when the latter are extreme.
31626297	6	35	theme	other	1366:1370	arg1	forces					1429:1434	more important selective forces	1404:1434	more important selective forces	1404:1434	The higher density and trend toward lower diversity in the cryopeg communities suggest that long-term stability and other features of a subzero brine are more important selective forces than in situ temperature or salinity, even when the latter are extreme.
31626297	6	35	theme	other	1366:1370	arg1	stability					1352:1360	long-term stability	1342:1360	long-term stability	1342:1360	The higher density and trend toward lower diversity in the cryopeg communities suggest that long-term stability and other features of a subzero brine are more important selective forces than in situ temperature or salinity, even when the latter are extreme.
31626297	6	35	theme	other	1366:1370	arg1	features					1372:1379	other features	1366:1379	other features of a subzero brine	1366:1398	The higher density and trend toward lower diversity in the cryopeg communities suggest that long-term stability and other features of a subzero brine are more important selective forces than in situ temperature or salinity, even when the latter are extreme.
31626297	0	36	from	ice	88:90	arg1	communities					22:32	Distinctive microbial communities	0:32	Distinctive microbial communities in subzero hypersaline brines from Arctic coastal sea ice	0:90	Distinctive microbial communities in subzero hypersaline brines from Arctic coastal sea ice and rarely sampled cryopegs.
31626297	0	36	from	ice	88:90	arg1	brines					57:62	subzero hypersaline brines	37:62	subzero hypersaline brines from Arctic coastal sea ice	37:90	Distinctive microbial communities in subzero hypersaline brines from Arctic coastal sea ice and rarely sampled cryopegs.
31626297	0	36	from	ice	88:90	arg1	cryopegs					111:118	rarely sampled cryopegs	96:118	rarely sampled cryopegs	96:118	Distinctive microbial communities in subzero hypersaline brines from Arctic coastal sea ice and rarely sampled cryopegs.
31626297	2	37	theme	Utqiaġvik	302:310	arg1	Alaska					313:318	Utqiaġvik, Alaska	302:318	Alaska	313:318	Near Utqiaġvik, Alaska, we sampled subzero brines (-6°C, 115-140 ppt) from cryopegs, i.e. unfrozen sediments within permafrost that contain relic (late Pleistocene) seawater brine, as well as nearby sea-ice brines to examine microbial community composition and diversity using 16S rRNA gene amplicon sequencing.
31626297	2	38	theme	unfrozen	387:394	arg1	sediments					396:404	unfrozen sediments	387:404	unfrozen sediments within permafrost that contain relic (late Pleistocene) seawater brine	387:475	Near Utqiaġvik, Alaska, we sampled subzero brines (-6°C, 115-140 ppt) from cryopegs, i.e. unfrozen sediments within permafrost that contain relic (late Pleistocene) seawater brine, as well as nearby sea-ice brines to examine microbial community composition and diversity using 16S rRNA gene amplicon sequencing.
31626297	0	39	theme	hypersaline	45:55	arg1	brines					57:62	subzero hypersaline brines	37:62	subzero hypersaline brines from Arctic coastal sea ice	37:90	Distinctive microbial communities in subzero hypersaline brines from Arctic coastal sea ice and rarely sampled cryopegs.
31626297	1	40	theme	Hypersaline	121:131	arg1	environments					141:152	Hypersaline aqueous environments	121:152	Hypersaline aqueous environments at subzero temperatures	121:176	Hypersaline aqueous environments at subzero temperatures are known to be inhabited by microorganisms, yet information on community structure in subzero brines is very limited.
31626297	5	41	theme	beta-diversity	1043:1056	arg1	indices					1058:1064	alpha- and beta-diversity indices	1032:1064	alpha- and beta-diversity indices	1032:1064	Community composition and diversity differed between the two brine environments by alpha- and beta-diversity indices, with cryopeg brine communities appearing less diverse and dominated by one strain of the genus Marinobacter, also detected in other cold, hypersaline environments, including sea ice.
31626297	4	42	dep	cells	827:831	arg1	to					820:821	to	820:821	to	820:821	The cryopeg brines harbored surprisingly dense bacterial communities (up to 108 cells mL-1) and millimolar levels of dissolved and particulate organic matter, extracellular polysaccharides and ammonia.
31626297	0	43	theme	subzero	37:43	arg1	brines					57:62	subzero hypersaline brines	37:62	subzero hypersaline brines from Arctic coastal sea ice	37:90	Distinctive microbial communities in subzero hypersaline brines from Arctic coastal sea ice and rarely sampled cryopegs.
31626297	6	44	dep	in	1441:1442	arg1	situ					1444:1447	situ	1444:1447	situ	1444:1447	The higher density and trend toward lower diversity in the cryopeg communities suggest that long-term stability and other features of a subzero brine are more important selective forces than in situ temperature or salinity, even when the latter are extreme.
31626297	2	45	theme	sea-ice	496:502	arg1	brines					504:509	nearby sea-ice brines	489:509	nearby sea-ice brines	489:509	Near Utqiaġvik, Alaska, we sampled subzero brines (-6°C, 115-140 ppt) from cryopegs, i.e. unfrozen sediments within permafrost that contain relic (late Pleistocene) seawater brine, as well as nearby sea-ice brines to examine microbial community composition and diversity using 16S rRNA gene amplicon sequencing.
31626297	4	46	theme	108	823:825	arg1	cells					827:831	cells	827:831	cells	827:831	The cryopeg brines harbored surprisingly dense bacterial communities (up to 108 cells mL-1) and millimolar levels of dissolved and particulate organic matter, extracellular polysaccharides and ammonia.
31626297	6	47	theme	brine	1394:1398	arg1	forces					1429:1434	more important selective forces	1404:1434	more important selective forces	1404:1434	The higher density and trend toward lower diversity in the cryopeg communities suggest that long-term stability and other features of a subzero brine are more important selective forces than in situ temperature or salinity, even when the latter are extreme.
31626297	6	47	theme	brine	1394:1398	arg1	stability					1352:1360	long-term stability	1342:1360	long-term stability	1342:1360	The higher density and trend toward lower diversity in the cryopeg communities suggest that long-term stability and other features of a subzero brine are more important selective forces than in situ temperature or salinity, even when the latter are extreme.
31626297	6	47	theme	brine	1394:1398	arg1	features					1372:1379	other features	1366:1379	other features of a subzero brine	1366:1398	The higher density and trend toward lower diversity in the cryopeg communities suggest that long-term stability and other features of a subzero brine are more important selective forces than in situ temperature or salinity, even when the latter are extreme.
31626297	6	48	theme	long-term	1342:1350	arg1	forces					1429:1434	more important selective forces	1404:1434	more important selective forces	1404:1434	The higher density and trend toward lower diversity in the cryopeg communities suggest that long-term stability and other features of a subzero brine are more important selective forces than in situ temperature or salinity, even when the latter are extreme.
31626297	6	48	theme	long-term	1342:1350	arg1	stability					1352:1360	long-term stability	1342:1360	long-term stability	1342:1360	The higher density and trend toward lower diversity in the cryopeg communities suggest that long-term stability and other features of a subzero brine are more important selective forces than in situ temperature or salinity, even when the latter are extreme.
31626297	6	48	theme	long-term	1342:1350	arg1	features					1372:1379	other features	1366:1379	other features of a subzero brine	1366:1398	The higher density and trend toward lower diversity in the cryopeg communities suggest that long-term stability and other features of a subzero brine are more important selective forces than in situ temperature or salinity, even when the latter are extreme.
31626297	6	49	theme	important	1409:1417	arg1	forces					1429:1434	more important selective forces	1404:1434	more important selective forces	1404:1434	The higher density and trend toward lower diversity in the cryopeg communities suggest that long-term stability and other features of a subzero brine are more important selective forces than in situ temperature or salinity, even when the latter are extreme.
31626297	6	49	theme	important	1409:1417	arg1	stability					1352:1360	long-term stability	1342:1360	long-term stability	1342:1360	The higher density and trend toward lower diversity in the cryopeg communities suggest that long-term stability and other features of a subzero brine are more important selective forces than in situ temperature or salinity, even when the latter are extreme.
31626297	6	49	theme	important	1409:1417	arg1	features					1372:1379	other features	1366:1379	other features of a subzero brine	1366:1398	The higher density and trend toward lower diversity in the cryopeg communities suggest that long-term stability and other features of a subzero brine are more important selective forces than in situ temperature or salinity, even when the latter are extreme.
31626297	2	50	dep	sediments	396:404	arg1	i.e.					382:385	i.e.	382:385	i.e.	382:385	Near Utqiaġvik, Alaska, we sampled subzero brines (-6°C, 115-140 ppt) from cryopegs, i.e. unfrozen sediments within permafrost that contain relic (late Pleistocene) seawater brine, as well as nearby sea-ice brines to examine microbial community composition and diversity using 16S rRNA gene amplicon sequencing.
31626297	2	51	theme	community	532:540	arg1	composition					542:552	microbial community composition	522:552	microbial community composition	522:552	Near Utqiaġvik, Alaska, we sampled subzero brines (-6°C, 115-140 ppt) from cryopegs, i.e. unfrozen sediments within permafrost that contain relic (late Pleistocene) seawater brine, as well as nearby sea-ice brines to examine microbial community composition and diversity using 16S rRNA gene amplicon sequencing.
31626297	5	52	theme	Marinobacter	1162:1173	arg1	strain					1142:1147	one strain	1138:1147	one strain of the genus Marinobacter	1138:1173	Community composition and diversity differed between the two brine environments by alpha- and beta-diversity indices, with cryopeg brine communities appearing less diverse and dominated by one strain of the genus Marinobacter, also detected in other cold, hypersaline environments, including sea ice.
31626297	2	53	theme	relic	437:441	arg1	brine					471:475	relic (late Pleistocene) seawater brine	437:475	relic (late Pleistocene) seawater brine	437:475	Near Utqiaġvik, Alaska, we sampled subzero brines (-6°C, 115-140 ppt) from cryopegs, i.e. unfrozen sediments within permafrost that contain relic (late Pleistocene) seawater brine, as well as nearby sea-ice brines to examine microbial community composition and diversity using 16S rRNA gene amplicon sequencing.
31626297	4	54	theme	matter	898:903	arg1	levels					854:859	millimolar levels	843:859	millimolar levels of dissolved and particulate organic matter, extracellular polysaccharides and ammonia	843:946	The cryopeg brines harbored surprisingly dense bacterial communities (up to 108 cells mL-1) and millimolar levels of dissolved and particulate organic matter, extracellular polysaccharides and ammonia.
31626297	4	54	theme	matter	898:903	arg1	communities					804:814	surprisingly dense bacterial communities	775:814	surprisingly dense bacterial communities (up to 108 cells mL-1)	775:837	The cryopeg brines harbored surprisingly dense bacterial communities (up to 108 cells mL-1) and millimolar levels of dissolved and particulate organic matter, extracellular polysaccharides and ammonia.
31626297	4	54	theme	matter	898:903	arg1	mL-1					833:836	up to 108 cells mL-1	817:836	up to 108 cells mL-1	817:836	The cryopeg brines harbored surprisingly dense bacterial communities (up to 108 cells mL-1) and millimolar levels of dissolved and particulate organic matter, extracellular polysaccharides and ammonia.
31626297	0	55	from	cryopegs	111:118	arg1	ice					88:90	Arctic coastal sea ice	69:90	Arctic coastal sea ice	69:90	Distinctive microbial communities in subzero hypersaline brines from Arctic coastal sea ice and rarely sampled cryopegs.
31626297	0	55	from	cryopegs	111:118	arg1	brines					57:62	subzero hypersaline brines	37:62	subzero hypersaline brines from Arctic coastal sea ice	37:90	Distinctive microbial communities in subzero hypersaline brines from Arctic coastal sea ice and rarely sampled cryopegs.
31626297	5	56	dep	cold	1199:1202	arg1	hypersaline					1205:1215	hypersaline	1205:1215	hypersaline	1205:1215	Community composition and diversity differed between the two brine environments by alpha- and beta-diversity indices, with cryopeg brine communities appearing less diverse and dominated by one strain of the genus Marinobacter, also detected in other cold, hypersaline environments, including sea ice.
31626297	2	57	from	cryopegs	372:379	arg1	-6°C					348:351	-6°C	348:351	-6°C	348:351	Near Utqiaġvik, Alaska, we sampled subzero brines (-6°C, 115-140 ppt) from cryopegs, i.e. unfrozen sediments within permafrost that contain relic (late Pleistocene) seawater brine, as well as nearby sea-ice brines to examine microbial community composition and diversity using 16S rRNA gene amplicon sequencing.
31626297	2	57	from	cryopegs	372:379	arg1	brines					340:345	subzero brines	332:345	subzero brines (-6°C, 115-140 ppt)	332:365	Near Utqiaġvik, Alaska, we sampled subzero brines (-6°C, 115-140 ppt) from cryopegs, i.e. unfrozen sediments within permafrost that contain relic (late Pleistocene) seawater brine, as well as nearby sea-ice brines to examine microbial community composition and diversity using 16S rRNA gene amplicon sequencing.
31626297	2	57	from	cryopegs	372:379	arg1	brines					504:509	nearby sea-ice brines	489:509	nearby sea-ice brines	489:509	Near Utqiaġvik, Alaska, we sampled subzero brines (-6°C, 115-140 ppt) from cryopegs, i.e. unfrozen sediments within permafrost that contain relic (late Pleistocene) seawater brine, as well as nearby sea-ice brines to examine microbial community composition and diversity using 16S rRNA gene amplicon sequencing.
31626297	2	57	from	cryopegs	372:379	arg1	sediments					396:404	unfrozen sediments	387:404	unfrozen sediments within permafrost that contain relic (late Pleistocene) seawater brine	387:475	Near Utqiaġvik, Alaska, we sampled subzero brines (-6°C, 115-140 ppt) from cryopegs, i.e. unfrozen sediments within permafrost that contain relic (late Pleistocene) seawater brine, as well as nearby sea-ice brines to examine microbial community composition and diversity using 16S rRNA gene amplicon sequencing.
31626297	2	58	theme	subzero	332:338	arg1	-6°C					348:351	-6°C	348:351	-6°C	348:351	Near Utqiaġvik, Alaska, we sampled subzero brines (-6°C, 115-140 ppt) from cryopegs, i.e. unfrozen sediments within permafrost that contain relic (late Pleistocene) seawater brine, as well as nearby sea-ice brines to examine microbial community composition and diversity using 16S rRNA gene amplicon sequencing.
31626297	2	58	theme	subzero	332:338	arg1	brines					340:345	subzero brines	332:345	subzero brines (-6°C, 115-140 ppt)	332:365	Near Utqiaġvik, Alaska, we sampled subzero brines (-6°C, 115-140 ppt) from cryopegs, i.e. unfrozen sediments within permafrost that contain relic (late Pleistocene) seawater brine, as well as nearby sea-ice brines to examine microbial community composition and diversity using 16S rRNA gene amplicon sequencing.
31626297	5	59	theme	other	1193:1197	arg1	ice					1245:1247	sea ice	1241:1247	sea ice	1241:1247	Community composition and diversity differed between the two brine environments by alpha- and beta-diversity indices, with cryopeg brine communities appearing less diverse and dominated by one strain of the genus Marinobacter, also detected in other cold, hypersaline environments, including sea ice.
31626297	5	59	theme	other	1193:1197	arg1	environments					1217:1228	other cold, hypersaline environments	1193:1228	other cold, hypersaline environments	1193:1228	Community composition and diversity differed between the two brine environments by alpha- and beta-diversity indices, with cryopeg brine communities appearing less diverse and dominated by one strain of the genus Marinobacter, also detected in other cold, hypersaline environments, including sea ice.
31626297	1	60	from	information	227:237	arg1	structure					252:260	community structure	242:260	community structure	242:260	Hypersaline aqueous environments at subzero temperatures are known to be inhabited by microorganisms, yet information on community structure in subzero brines is very limited.
31626297	1	60	from	information	227:237	arg1	brines					273:278	subzero brines	265:278	subzero brines	265:278	Hypersaline aqueous environments at subzero temperatures are known to be inhabited by microorganisms, yet information on community structure in subzero brines is very limited.
31626297	5	61	theme	brine	1080:1084	arg1	communities					1086:1096	cryopeg brine communities	1072:1096	cryopeg brine communities	1072:1096	Community composition and diversity differed between the two brine environments by alpha- and beta-diversity indices, with cryopeg brine communities appearing less diverse and dominated by one strain of the genus Marinobacter, also detected in other cold, hypersaline environments, including sea ice.
31626297	6	62	theme	higher	1254:1259	arg1	density					1261:1267	The higher density	1250:1267	The higher density	1250:1267	The higher density and trend toward lower diversity in the cryopeg communities suggest that long-term stability and other features of a subzero brine are more important selective forces than in situ temperature or salinity, even when the latter are extreme.
31626297	1	63	theme	aqueous	133:139	arg1	environments					141:152	Hypersaline aqueous environments	121:152	Hypersaline aqueous environments at subzero temperatures	121:176	Hypersaline aqueous environments at subzero temperatures are known to be inhabited by microorganisms, yet information on community structure in subzero brines is very limited.
31626297	2	64	theme	microbial	522:530	arg1	composition					542:552	microbial community composition	522:552	microbial community composition	522:552	Near Utqiaġvik, Alaska, we sampled subzero brines (-6°C, 115-140 ppt) from cryopegs, i.e. unfrozen sediments within permafrost that contain relic (late Pleistocene) seawater brine, as well as nearby sea-ice brines to examine microbial community composition and diversity using 16S rRNA gene amplicon sequencing.
31626297	6	65	theme	lower	1286:1290	arg1	diversity					1292:1300	lower diversity	1286:1300	lower diversity in the cryopeg communities	1286:1327	The higher density and trend toward lower diversity in the cryopeg communities suggest that long-term stability and other features of a subzero brine are more important selective forces than in situ temperature or salinity, even when the latter are extreme.
31626297	2	66	theme	late	444:447	arg1	Pleistocene					449:459	late Pleistocene	444:459	late Pleistocene	444:459	Near Utqiaġvik, Alaska, we sampled subzero brines (-6°C, 115-140 ppt) from cryopegs, i.e. unfrozen sediments within permafrost that contain relic (late Pleistocene) seawater brine, as well as nearby sea-ice brines to examine microbial community composition and diversity using 16S rRNA gene amplicon sequencing.
31626297	5	67	theme	Community	949:957	arg1	composition					959:969	Community composition	949:969	Community composition	949:969	Community composition and diversity differed between the two brine environments by alpha- and beta-diversity indices, with cryopeg brine communities appearing less diverse and dominated by one strain of the genus Marinobacter, also detected in other cold, hypersaline environments, including sea ice.
31626297	6	68	from	diversity	1292:1300	arg1	communities					1317:1327	the cryopeg communities	1305:1327	the cryopeg communities	1305:1327	The higher density and trend toward lower diversity in the cryopeg communities suggest that long-term stability and other features of a subzero brine are more important selective forces than in situ temperature or salinity, even when the latter are extreme.
31626297	0	69	theme	coastal	76:82	arg1	ice					88:90	Arctic coastal sea ice	69:90	Arctic coastal sea ice	69:90	Distinctive microbial communities in subzero hypersaline brines from Arctic coastal sea ice and rarely sampled cryopegs.
31626297	5	70	theme	alpha-	1032:1037	arg1	indices					1058:1064	alpha- and beta-diversity indices	1032:1064	alpha- and beta-diversity indices	1032:1064	Community composition and diversity differed between the two brine environments by alpha- and beta-diversity indices, with cryopeg brine communities appearing less diverse and dominated by one strain of the genus Marinobacter, also detected in other cold, hypersaline environments, including sea ice.
31626297	6	71	theme	in	1441:1442	arg1	temperature					1449:1459	in situ temperature	1441:1459	in situ temperature	1441:1459	The higher density and trend toward lower diversity in the cryopeg communities suggest that long-term stability and other features of a subzero brine are more important selective forces than in situ temperature or salinity, even when the latter are extreme.
31626297	1	72	theme	community	242:250	arg1	structure					252:260	community structure	242:260	community structure	242:260	Hypersaline aqueous environments at subzero temperatures are known to be inhabited by microorganisms, yet information on community structure in subzero brines is very limited.
31626297	0	73	theme	Arctic	69:74	arg1	ice					88:90	Arctic coastal sea ice	69:90	Arctic coastal sea ice	69:90	Distinctive microbial communities in subzero hypersaline brines from Arctic coastal sea ice and rarely sampled cryopegs.
31626297	3	74	theme	possible	701:708	arg1	parameters					687:696	environmental parameters	673:696	environmental parameters	673:696	We also quantified the communities microscopically and assessed environmental parameters as possible determinants of community structure.
31626297	3	74	theme	possible	701:708	arg1	determinants					710:721	possible determinants	701:721	possible determinants of community structure	701:744	We also quantified the communities microscopically and assessed environmental parameters as possible determinants of community structure.
31626297	1	75	theme	subzero	157:163	arg1	temperatures					165:176	subzero temperatures	157:176	subzero temperatures	157:176	Hypersaline aqueous environments at subzero temperatures are known to be inhabited by microorganisms, yet information on community structure in subzero brines is very limited.
31626297	5	76	dep	diverse	1113:1119	arg1	detected					1181:1188	detected	1181:1188	detected in other cold, hypersaline environments, including sea ice	1181:1247	Community composition and diversity differed between the two brine environments by alpha- and beta-diversity indices, with cryopeg brine communities appearing less diverse and dominated by one strain of the genus Marinobacter, also detected in other cold, hypersaline environments, including sea ice.
29492107	10	0	theme	mature	1679:1684	arg1	tissues					1686:1692	mature tissues	1679:1692	mature tissues	1679:1692	Lignin influence was highlighted by the strong negative correlation with fluorescence intensity which mainly originates from monolignols in mature tissues.
29492107	12	1	theme	enzyme	1970:1975	arg1	size					1977:1980	fitted enzyme size	1963:1980	fitted enzyme size	1963:1980	The correlations with specific porosity ranges are biomass species-dependent, meaning that enzymes cocktails with fitted enzyme size are likely to be needed to optimise saccharification depending on the biomass origin.
29492107	11	2	theme	biomass	1781:1787	arg1	generic					1840:1846	generic	1840:1846	generic	1840:1846	CONCLUSIONS Our results provide a better understanding of the factors responsible for biomass recalcitrance that can reasonably be considered as generic.
29492107	11	2	theme	biomass	1781:1787	arg1	recalcitrance					1789:1801	biomass recalcitrance	1781:1801	biomass recalcitrance that can reasonably be considered as generic	1781:1846	CONCLUSIONS Our results provide a better understanding of the factors responsible for biomass recalcitrance that can reasonably be considered as generic.
29492107	7	3	theme	saccharification	1193:1208	arg1	efficiency					1210:1219	saccharification efficiency	1193:1219	saccharification efficiency	1193:1219	Increases in porosity displayed overall strong positive correlations with saccharification efficiency, but different porosity ranges were concerned depending on the considered biomass.
29492107	3	4	theme	contrasted	506:515	arg1	samples					517:523	contrasted samples	506:523	contrasted samples	506:523	Three representative biomass species (wheat straw, poplar and miscanthus) were submitted to four standard pretreatments (dilute acid, hot water, ionic liquid and sodium chlorite) in order to generate a set of contrasted samples.
29492107	4	5	theme	porosity	589:596	arg1	measurements					598:609	porosity measurements	589:609	porosity measurements using NMR spectroscopy	589:632	A large array of techniques, including wet chemistry analysis, porosity measurements using NMR spectroscopy, electron and fluorescence microscopy, were used in order to determine possible generic factors of biomass recalcitrance.
29492107	13	6	theme	important	2141:2149	arg1	composition					2075:2085	Lignin composition	2068:2085	Lignin composition	2068:2085	Lignin composition, which probably influences its structure, is the most important parameter to overcome to enhance enzymes access to the polysaccharides.
29492107	13	6	theme	important	2141:2149	arg1	parameter					2151:2159	the most important parameter	2132:2159	the most important parameter to overcome to enhance enzymes access to the polysaccharides	2132:2220	Lignin composition, which probably influences its structure, is the most important parameter to overcome to enhance enzymes access to the polysaccharides.
29492107	12	7	with	cocktails	1948:1956	arg1	size					1977:1980	fitted enzyme size	1963:1980	fitted enzyme size	1963:1980	The correlations with specific porosity ranges are biomass species-dependent, meaning that enzymes cocktails with fitted enzyme size are likely to be needed to optimise saccharification depending on the biomass origin.
29492107	12	8	theme	enzymes	1940:1946	arg1	cocktails					1948:1956	enzymes cocktails	1940:1956	enzymes cocktails with fitted enzyme size	1940:1980	The correlations with specific porosity ranges are biomass species-dependent, meaning that enzymes cocktails with fitted enzyme size are likely to be needed to optimise saccharification depending on the biomass origin.
29492107	8	9	theme	negative	1344:1351	arg1	coefficients					1353:1364	highly negative coefficients	1337:1364	highly negative coefficients for all biomasses	1337:1382	Lignin-related factors displayed highly negative coefficients for all biomasses.
29492107	7	10	theme	strong	1159:1164	arg1	correlations					1175:1186	overall strong positive correlations	1151:1186	overall strong positive correlations with saccharification efficiency	1151:1219	Increases in porosity displayed overall strong positive correlations with saccharification efficiency, but different porosity ranges were concerned depending on the considered biomass.
29492107	10	11	theme	Lignin	1539:1544	arg1	influence					1546:1554	Lignin influence	1539:1554	Lignin influence	1539:1554	Lignin influence was highlighted by the strong negative correlation with fluorescence intensity which mainly originates from monolignols in mature tissues.
29492107	12	12	theme	species-dependent	1908:1924	arg1	correlations					1853:1864	The correlations	1849:1864	The correlations with specific porosity ranges	1849:1894	The correlations with specific porosity ranges are biomass species-dependent, meaning that enzymes cocktails with fitted enzyme size are likely to be needed to optimise saccharification depending on the biomass origin.
29492107	12	12	theme	species-dependent	1908:1924	arg1	meaning					1927:1933	biomass species-dependent, meaning	1900:1933	meaning	1927:1933	The correlations with specific porosity ranges are biomass species-dependent, meaning that enzymes cocktails with fitted enzyme size are likely to be needed to optimise saccharification depending on the biomass origin.
29492107	5	13	theme	saccharification	959:974	arg1	96 h					951:954	96 h	951:954	96 h of saccharification	951:974	RESULTS The pretreatment conditions selected allowed obtaining samples displaying different susceptibility to enzymatic hydrolysis (from 3 up to 98% of the initial glucose content released after 96 h of saccharification).
29492107	8	14	theme	Lignin-related	1304:1317	arg1	factors					1319:1325	Lignin-related factors	1304:1325	Lignin-related factors	1304:1325	Lignin-related factors displayed highly negative coefficients for all biomasses.
29492107	6	15	theme	Generic	978:984	arg1	coefficients					998:1009	Generic correlation coefficients	978:1009	Generic correlation coefficients	978:1009	Generic correlation coefficients were calculated between the measured chemical and structural features and the final saccharification rates.
29492107	3	16	theme	representative	303:316	arg1	species					326:332	Three representative biomass species	297:332	Three representative biomass species (wheat straw, poplar and miscanthus)	297:369	Three representative biomass species (wheat straw, poplar and miscanthus) were submitted to four standard pretreatments (dilute acid, hot water, ionic liquid and sodium chlorite) in order to generate a set of contrasted samples.
29492107	3	16	theme	representative	303:316	arg1	straw					341:345	wheat straw	335:345	wheat straw	335:345	Three representative biomass species (wheat straw, poplar and miscanthus) were submitted to four standard pretreatments (dilute acid, hot water, ionic liquid and sodium chlorite) in order to generate a set of contrasted samples.
29492107	3	16	theme	representative	303:316	arg1	miscanthus					359:368	miscanthus	359:368	miscanthus	359:368	Three representative biomass species (wheat straw, poplar and miscanthus) were submitted to four standard pretreatments (dilute acid, hot water, ionic liquid and sodium chlorite) in order to generate a set of contrasted samples.
29492107	3	16	theme	representative	303:316	arg1	poplar					348:353	poplar	348:353	poplar	348:353	Three representative biomass species (wheat straw, poplar and miscanthus) were submitted to four standard pretreatments (dilute acid, hot water, ionic liquid and sodium chlorite) in order to generate a set of contrasted samples.
29492107	4	17	theme	fluorescence	648:659	arg1	microscopy					661:670	fluorescence microscopy	648:670	fluorescence microscopy	648:670	A large array of techniques, including wet chemistry analysis, porosity measurements using NMR spectroscopy, electron and fluorescence microscopy, were used in order to determine possible generic factors of biomass recalcitrance.
29492107	7	18	theme	porosity	1236:1243	arg1	ranges					1245:1250	different porosity ranges	1226:1250	different porosity ranges	1226:1250	Increases in porosity displayed overall strong positive correlations with saccharification efficiency, but different porosity ranges were concerned depending on the considered biomass.
29492107	4	19	theme	generic	714:720	arg1	factors					722:728	possible generic factors	705:728	possible generic factors of biomass recalcitrance	705:753	A large array of techniques, including wet chemistry analysis, porosity measurements using NMR spectroscopy, electron and fluorescence microscopy, were used in order to determine possible generic factors of biomass recalcitrance.
29492107	2	20	theme	relative	244:251	arg1	importance					253:262	their relative importance	238:262	their relative importance	238:262	However, their relative importance remains challenging to evaluate.
29492107	1	21	theme	enzymatic	146:154	arg1	hydrolysis					156:165	enzymatic hydrolysis	146:165	enzymatic hydrolysis	146:165	BACKGROUND Biomass recalcitrance to enzymatic hydrolysis has been assigned to several structural and chemical factors.
29492107	12	22	theme	specific	1871:1878	arg1	ranges					1889:1894	specific porosity ranges	1871:1894	specific porosity ranges	1871:1894	The correlations with specific porosity ranges are biomass species-dependent, meaning that enzymes cocktails with fitted enzyme size are likely to be needed to optimise saccharification depending on the biomass origin.
29492107	3	23	theme	wheat	335:339	arg1	species					326:332	Three representative biomass species	297:332	Three representative biomass species (wheat straw, poplar and miscanthus)	297:369	Three representative biomass species (wheat straw, poplar and miscanthus) were submitted to four standard pretreatments (dilute acid, hot water, ionic liquid and sodium chlorite) in order to generate a set of contrasted samples.
29492107	3	23	theme	wheat	335:339	arg1	straw					341:345	wheat straw	335:345	wheat straw	335:345	Three representative biomass species (wheat straw, poplar and miscanthus) were submitted to four standard pretreatments (dilute acid, hot water, ionic liquid and sodium chlorite) in order to generate a set of contrasted samples.
29492107	6	24	theme	structural	1061:1070	arg1	features					1072:1079	the measured chemical and structural features	1035:1079	the measured chemical and structural features	1035:1079	Generic correlation coefficients were calculated between the measured chemical and structural features and the final saccharification rates.
29492107	7	25	from	Increases	1119:1127	arg1	porosity					1132:1139	porosity	1132:1139	porosity	1132:1139	Increases in porosity displayed overall strong positive correlations with saccharification efficiency, but different porosity ranges were concerned depending on the considered biomass.
29492107	6	26	theme	chemical	1048:1055	arg1	features					1072:1079	the measured chemical and structural features	1035:1079	the measured chemical and structural features	1035:1079	Generic correlation coefficients were calculated between the measured chemical and structural features and the final saccharification rates.
29492107	3	27	dep	pretreatments	403:415	arg1	pretreatments					403:415	four standard pretreatments	389:415	four standard pretreatments (dilute acid, hot water, ionic liquid and sodium chlorite)	389:474	Three representative biomass species (wheat straw, poplar and miscanthus) were submitted to four standard pretreatments (dilute acid, hot water, ionic liquid and sodium chlorite) in order to generate a set of contrasted samples.
29492107	3	27	dep	pretreatments	403:415	arg1	acid					425:428	dilute acid	418:428	dilute acid	418:428	Three representative biomass species (wheat straw, poplar and miscanthus) were submitted to four standard pretreatments (dilute acid, hot water, ionic liquid and sodium chlorite) in order to generate a set of contrasted samples.
29492107	3	27	dep	pretreatments	403:415	arg1	water					435:439	hot water	431:439	hot water	431:439	Three representative biomass species (wheat straw, poplar and miscanthus) were submitted to four standard pretreatments (dilute acid, hot water, ionic liquid and sodium chlorite) in order to generate a set of contrasted samples.
29492107	3	27	dep	pretreatments	403:415	arg1	liquid					448:453	liquid	448:453	liquid	448:453	Three representative biomass species (wheat straw, poplar and miscanthus) were submitted to four standard pretreatments (dilute acid, hot water, ionic liquid and sodium chlorite) in order to generate a set of contrasted samples.
29492107	3	27	dep	pretreatments	403:415	arg1	chlorite					466:473	sodium chlorite	459:473	sodium chlorite	459:473	Three representative biomass species (wheat straw, poplar and miscanthus) were submitted to four standard pretreatments (dilute acid, hot water, ionic liquid and sodium chlorite) in order to generate a set of contrasted samples.
29492107	0	28	theme	lignocellulose	80:93	arg1	recalcitrance					95:107	lignocellulose recalcitrance	80:107	lignocellulose recalcitrance	80:107	Multimodal analysis of pretreated biomass species highlights generic markers of lignocellulose recalcitrance.
29492107	5	29	theme	glucose	920:926	arg1	content					928:934	the initial glucose content	908:934	the initial glucose content released after 96 h of saccharification	908:974	RESULTS The pretreatment conditions selected allowed obtaining samples displaying different susceptibility to enzymatic hydrolysis (from 3 up to 98% of the initial glucose content released after 96 h of saccharification).
29492107	14	30	theme	simple	2287:2292	arg1	intensity					2249:2257	fluorescence intensity	2236:2257	fluorescence intensity	2236:2257	Accordingly, fluorescence intensity was found to be a rapid and simple method to assess recalcitrance after pretreatment.
29492107	14	30	theme	simple	2287:2292	arg1	method					2294:2299	a rapid and simple method	2275:2299	a rapid and simple method to assess recalcitrance after pretreatment	2275:2342	Accordingly, fluorescence intensity was found to be a rapid and simple method to assess recalcitrance after pretreatment.
29492107	12	31	dep	meaning	1927:1933	arg1	likely					1986:1991	likely	1986:1991	likely	1986:1991	The correlations with specific porosity ranges are biomass species-dependent, meaning that enzymes cocktails with fitted enzyme size are likely to be needed to optimise saccharification depending on the biomass origin.
29492107	14	32	theme	rapid	2277:2281	arg1	intensity					2249:2257	fluorescence intensity	2236:2257	fluorescence intensity	2236:2257	Accordingly, fluorescence intensity was found to be a rapid and simple method to assess recalcitrance after pretreatment.
29492107	14	32	theme	rapid	2277:2281	arg1	method					2294:2299	a rapid and simple method	2275:2299	a rapid and simple method to assess recalcitrance after pretreatment	2275:2342	Accordingly, fluorescence intensity was found to be a rapid and simple method to assess recalcitrance after pretreatment.
29492107	3	33	theme	dilute	418:423	arg1	pretreatments					403:415	four standard pretreatments	389:415	four standard pretreatments (dilute acid, hot water, ionic liquid and sodium chlorite)	389:474	Three representative biomass species (wheat straw, poplar and miscanthus) were submitted to four standard pretreatments (dilute acid, hot water, ionic liquid and sodium chlorite) in order to generate a set of contrasted samples.
29492107	3	33	theme	dilute	418:423	arg1	acid					425:428	dilute acid	418:428	dilute acid	418:428	Three representative biomass species (wheat straw, poplar and miscanthus) were submitted to four standard pretreatments (dilute acid, hot water, ionic liquid and sodium chlorite) in order to generate a set of contrasted samples.
29492107	10	34	theme	strong	1579:1584	arg1	correlation					1595:1605	the strong negative correlation	1575:1605	the strong negative correlation with fluorescence intensity which mainly originates from monolignols in mature tissues	1575:1692	Lignin influence was highlighted by the strong negative correlation with fluorescence intensity which mainly originates from monolignols in mature tissues.
29492107	4	35	theme	recalcitrance	741:753	arg1	factors					722:728	possible generic factors	705:728	possible generic factors of biomass recalcitrance	705:753	A large array of techniques, including wet chemistry analysis, porosity measurements using NMR spectroscopy, electron and fluorescence microscopy, were used in order to determine possible generic factors of biomass recalcitrance.
29492107	0	36	theme	Multimodal	0:9	arg1	analysis					11:18	Multimodal analysis	0:18	Multimodal analysis of pretreated biomass species	0:48	Multimodal analysis of pretreated biomass species highlights generic markers of lignocellulose recalcitrance.
29492107	1	37	theme	BACKGROUND	110:119	arg1	recalcitrance					129:141	BACKGROUND Biomass recalcitrance	110:141	BACKGROUND Biomass recalcitrance to enzymatic hydrolysis	110:165	BACKGROUND Biomass recalcitrance to enzymatic hydrolysis has been assigned to several structural and chemical factors.
29492107	4	38	theme	techniques	543:552	arg1	array					534:538	A large array	526:538	A large array of techniques, including wet chemistry analysis, porosity measurements using NMR spectroscopy, electron and fluorescence microscopy,	526:671	A large array of techniques, including wet chemistry analysis, porosity measurements using NMR spectroscopy, electron and fluorescence microscopy, were used in order to determine possible generic factors of biomass recalcitrance.
29492107	5	39	theme	enzymatic	866:874	arg1	hydrolysis					876:885	enzymatic hydrolysis	866:885	enzymatic hydrolysis (from 3 up to 98% of the initial glucose content released after 96 h of saccharification)	866:975	RESULTS The pretreatment conditions selected allowed obtaining samples displaying different susceptibility to enzymatic hydrolysis (from 3 up to 98% of the initial glucose content released after 96 h of saccharification).
29492107	10	40	with	correlation	1595:1605	arg1	intensity					1625:1633	fluorescence intensity	1612:1633	fluorescence intensity	1612:1633	Lignin influence was highlighted by the strong negative correlation with fluorescence intensity which mainly originates from monolignols in mature tissues.
29492107	6	41	theme	final	1089:1093	arg1	rates					1112:1116	the final saccharification rates	1085:1116	the final saccharification rates	1085:1116	Generic correlation coefficients were calculated between the measured chemical and structural features and the final saccharification rates.
29492107	10	42	theme	fluorescence	1612:1623	arg1	intensity					1625:1633	fluorescence intensity	1612:1633	fluorescence intensity	1612:1633	Lignin influence was highlighted by the strong negative correlation with fluorescence intensity which mainly originates from monolignols in mature tissues.
29492107	5	43	dep	98	901:902	arg1	to					898:899	to	898:899	to	898:899	RESULTS The pretreatment conditions selected allowed obtaining samples displaying different susceptibility to enzymatic hydrolysis (from 3 up to 98% of the initial glucose content released after 96 h of saccharification).
29492107	7	44	theme	different	1226:1234	arg1	ranges					1245:1250	different porosity ranges	1226:1250	different porosity ranges	1226:1250	Increases in porosity displayed overall strong positive correlations with saccharification efficiency, but different porosity ranges were concerned depending on the considered biomass.
29492107	0	45	theme	biomass	34:40	arg1	species					42:48	pretreated biomass species	23:48	pretreated biomass species	23:48	Multimodal analysis of pretreated biomass species highlights generic markers of lignocellulose recalcitrance.
29492107	5	46	theme	pretreatment	768:779	arg1	conditions					781:790	The pretreatment conditions	764:790	The pretreatment conditions	764:790	RESULTS The pretreatment conditions selected allowed obtaining samples displaying different susceptibility to enzymatic hydrolysis (from 3 up to 98% of the initial glucose content released after 96 h of saccharification).
29492107	11	47	theme	better	1729:1734	arg1	understanding					1736:1748	a better understanding	1727:1748	a better understanding of the factors responsible for biomass recalcitrance that can reasonably be considered as generic	1727:1846	CONCLUSIONS Our results provide a better understanding of the factors responsible for biomass recalcitrance that can reasonably be considered as generic.
29492107	12	48	theme	fitted	1963:1968	arg1	size					1977:1980	fitted enzyme size	1963:1980	fitted enzyme size	1963:1980	The correlations with specific porosity ranges are biomass species-dependent, meaning that enzymes cocktails with fitted enzyme size are likely to be needed to optimise saccharification depending on the biomass origin.
29492107	5	49	dep	hydrolysis	876:885	arg1	content					928:934	the initial glucose content	908:934	the initial glucose content released after 96 h of saccharification	908:974	RESULTS The pretreatment conditions selected allowed obtaining samples displaying different susceptibility to enzymatic hydrolysis (from 3 up to 98% of the initial glucose content released after 96 h of saccharification).
29492107	5	49	dep	hydrolysis	876:885	arg1	%					903:903	from 3 up to 98%	888:903	from 3 up to 98% of the initial glucose content released after 96 h of saccharification	888:974	RESULTS The pretreatment conditions selected allowed obtaining samples displaying different susceptibility to enzymatic hydrolysis (from 3 up to 98% of the initial glucose content released after 96 h of saccharification).
29492107	3	50	theme	samples	517:523	arg1	set					499:501	a set	497:501	a set of contrasted samples	497:523	Three representative biomass species (wheat straw, poplar and miscanthus) were submitted to four standard pretreatments (dilute acid, hot water, ionic liquid and sodium chlorite) in order to generate a set of contrasted samples.
29492107	3	50	theme	samples	517:523	arg1	samples					517:523	contrasted samples	506:523	contrasted samples	506:523	Three representative biomass species (wheat straw, poplar and miscanthus) were submitted to four standard pretreatments (dilute acid, hot water, ionic liquid and sodium chlorite) in order to generate a set of contrasted samples.
29492107	7	51	with	correlations	1175:1186	arg1	efficiency					1210:1219	saccharification efficiency	1193:1219	saccharification efficiency	1193:1219	Increases in porosity displayed overall strong positive correlations with saccharification efficiency, but different porosity ranges were concerned depending on the considered biomass.
29492107	11	52	dep	CONCLUSIONS	1695:1705	arg1	provide					1719:1725	provide	1719:1725	provide a better understanding of the factors responsible for biomass recalcitrance that can reasonably be considered as generic	1719:1846	CONCLUSIONS Our results provide a better understanding of the factors responsible for biomass recalcitrance that can reasonably be considered as generic.
29492107	4	53	theme	NMR	617:619	arg1	spectroscopy					621:632	NMR spectroscopy	617:632	NMR spectroscopy	617:632	A large array of techniques, including wet chemistry analysis, porosity measurements using NMR spectroscopy, electron and fluorescence microscopy, were used in order to determine possible generic factors of biomass recalcitrance.
29492107	3	54	dep	species	326:332	arg1	species					326:332	Three representative biomass species	297:332	Three representative biomass species (wheat straw, poplar and miscanthus)	297:369	Three representative biomass species (wheat straw, poplar and miscanthus) were submitted to four standard pretreatments (dilute acid, hot water, ionic liquid and sodium chlorite) in order to generate a set of contrasted samples.
29492107	3	54	dep	species	326:332	arg1	straw					341:345	wheat straw	335:345	wheat straw	335:345	Three representative biomass species (wheat straw, poplar and miscanthus) were submitted to four standard pretreatments (dilute acid, hot water, ionic liquid and sodium chlorite) in order to generate a set of contrasted samples.
29492107	3	54	dep	species	326:332	arg1	miscanthus					359:368	miscanthus	359:368	miscanthus	359:368	Three representative biomass species (wheat straw, poplar and miscanthus) were submitted to four standard pretreatments (dilute acid, hot water, ionic liquid and sodium chlorite) in order to generate a set of contrasted samples.
29492107	3	54	dep	species	326:332	arg1	poplar					348:353	poplar	348:353	poplar	348:353	Three representative biomass species (wheat straw, poplar and miscanthus) were submitted to four standard pretreatments (dilute acid, hot water, ionic liquid and sodium chlorite) in order to generate a set of contrasted samples.
29492107	11	55	theme	factors	1757:1763	arg1	understanding					1736:1748	a better understanding	1727:1748	a better understanding of the factors responsible for biomass recalcitrance that can reasonably be considered as generic	1727:1846	CONCLUSIONS Our results provide a better understanding of the factors responsible for biomass recalcitrance that can reasonably be considered as generic.
29492107	9	56	theme	lignin	1519:1524	arg1	composition					1526:1536	lignin composition	1519:1536	lignin composition	1519:1536	Lignin content, which is likely involved in the correlations observed for porosity, was less detrimental to enzymatic hydrolysis than lignin composition.
29492107	4	57	theme	chemistry	569:577	arg1	analysis					579:586	wet chemistry analysis	565:586	wet chemistry analysis	565:586	A large array of techniques, including wet chemistry analysis, porosity measurements using NMR spectroscopy, electron and fluorescence microscopy, were used in order to determine possible generic factors of biomass recalcitrance.
29492107	7	58	theme	positive	1166:1173	arg1	correlations					1175:1186	overall strong positive correlations	1151:1186	overall strong positive correlations with saccharification efficiency	1151:1219	Increases in porosity displayed overall strong positive correlations with saccharification efficiency, but different porosity ranges were concerned depending on the considered biomass.
29492107	7	59	theme	overall	1151:1157	arg1	correlations					1175:1186	overall strong positive correlations	1151:1186	overall strong positive correlations with saccharification efficiency	1151:1219	Increases in porosity displayed overall strong positive correlations with saccharification efficiency, but different porosity ranges were concerned depending on the considered biomass.
29492107	1	60	theme	structural	196:205	arg1	factors					220:226	several structural and chemical factors	188:226	several structural and chemical factors	188:226	BACKGROUND Biomass recalcitrance to enzymatic hydrolysis has been assigned to several structural and chemical factors.
29492107	12	61	theme	biomass	1900:1906	arg1	correlations					1853:1864	The correlations	1849:1864	The correlations with specific porosity ranges	1849:1894	The correlations with specific porosity ranges are biomass species-dependent, meaning that enzymes cocktails with fitted enzyme size are likely to be needed to optimise saccharification depending on the biomass origin.
29492107	12	61	theme	biomass	1900:1906	arg1	meaning					1927:1933	biomass species-dependent, meaning	1900:1933	meaning	1927:1933	The correlations with specific porosity ranges are biomass species-dependent, meaning that enzymes cocktails with fitted enzyme size are likely to be needed to optimise saccharification depending on the biomass origin.
29492107	1	62	theme	chemical	211:218	arg1	factors					220:226	several structural and chemical factors	188:226	several structural and chemical factors	188:226	BACKGROUND Biomass recalcitrance to enzymatic hydrolysis has been assigned to several structural and chemical factors.
29492107	6	63	theme	correlation	986:996	arg1	coefficients					998:1009	Generic correlation coefficients	978:1009	Generic correlation coefficients	978:1009	Generic correlation coefficients were calculated between the measured chemical and structural features and the final saccharification rates.
29492107	5	64	dep	selected	792:799	arg1	allowed					801:807	allowed	801:807	selected allowed obtaining samples displaying different susceptibility to enzymatic hydrolysis (from 3 up to 98% of the initial glucose content released after 96 h of saccharification)	792:975	RESULTS The pretreatment conditions selected allowed obtaining samples displaying different susceptibility to enzymatic hydrolysis (from 3 up to 98% of the initial glucose content released after 96 h of saccharification).
29492107	1	65	theme	several	188:194	arg1	factors					220:226	several structural and chemical factors	188:226	several structural and chemical factors	188:226	BACKGROUND Biomass recalcitrance to enzymatic hydrolysis has been assigned to several structural and chemical factors.
29492107	3	66	theme	biomass	318:324	arg1	species					326:332	Three representative biomass species	297:332	Three representative biomass species (wheat straw, poplar and miscanthus)	297:369	Three representative biomass species (wheat straw, poplar and miscanthus) were submitted to four standard pretreatments (dilute acid, hot water, ionic liquid and sodium chlorite) in order to generate a set of contrasted samples.
29492107	3	66	theme	biomass	318:324	arg1	straw					341:345	wheat straw	335:345	wheat straw	335:345	Three representative biomass species (wheat straw, poplar and miscanthus) were submitted to four standard pretreatments (dilute acid, hot water, ionic liquid and sodium chlorite) in order to generate a set of contrasted samples.
29492107	3	66	theme	biomass	318:324	arg1	miscanthus					359:368	miscanthus	359:368	miscanthus	359:368	Three representative biomass species (wheat straw, poplar and miscanthus) were submitted to four standard pretreatments (dilute acid, hot water, ionic liquid and sodium chlorite) in order to generate a set of contrasted samples.
29492107	3	66	theme	biomass	318:324	arg1	poplar					348:353	poplar	348:353	poplar	348:353	Three representative biomass species (wheat straw, poplar and miscanthus) were submitted to four standard pretreatments (dilute acid, hot water, ionic liquid and sodium chlorite) in order to generate a set of contrasted samples.
29492107	4	67	used	used	678:681	arg2	array					534:538	A large array	526:538	A large array of techniques, including wet chemistry analysis, porosity measurements using NMR spectroscopy, electron and fluorescence microscopy,	526:671	A large array of techniques, including wet chemistry analysis, porosity measurements using NMR spectroscopy, electron and fluorescence microscopy, were used in order to determine possible generic factors of biomass recalcitrance.
29492107	5	68	theme	content	928:934	arg1	content					928:934	the initial glucose content	908:934	the initial glucose content released after 96 h of saccharification	908:974	RESULTS The pretreatment conditions selected allowed obtaining samples displaying different susceptibility to enzymatic hydrolysis (from 3 up to 98% of the initial glucose content released after 96 h of saccharification).
29492107	5	68	theme	content	928:934	arg1	%					903:903	from 3 up to 98%	888:903	from 3 up to 98% of the initial glucose content released after 96 h of saccharification	888:974	RESULTS The pretreatment conditions selected allowed obtaining samples displaying different susceptibility to enzymatic hydrolysis (from 3 up to 98% of the initial glucose content released after 96 h of saccharification).
29492107	5	69	dep	RESULTS	756:762	arg1	selected					792:799	selected	792:799	selected allowed obtaining samples displaying different susceptibility to enzymatic hydrolysis (from 3 up to 98% of the initial glucose content released after 96 h of saccharification)	792:975	RESULTS The pretreatment conditions selected allowed obtaining samples displaying different susceptibility to enzymatic hydrolysis (from 3 up to 98% of the initial glucose content released after 96 h of saccharification).
29492107	4	70	theme	possible	705:712	arg1	factors					722:728	possible generic factors	705:728	possible generic factors of biomass recalcitrance	705:753	A large array of techniques, including wet chemistry analysis, porosity measurements using NMR spectroscopy, electron and fluorescence microscopy, were used in order to determine possible generic factors of biomass recalcitrance.
29492107	9	71	theme	Lignin	1385:1390	arg1	content					1392:1398	Lignin content	1385:1398	Lignin content	1385:1398	Lignin content, which is likely involved in the correlations observed for porosity, was less detrimental to enzymatic hydrolysis than lignin composition.
29492107	12	72	theme	porosity	1880:1887	arg1	ranges					1889:1894	specific porosity ranges	1871:1894	specific porosity ranges	1871:1894	The correlations with specific porosity ranges are biomass species-dependent, meaning that enzymes cocktails with fitted enzyme size are likely to be needed to optimise saccharification depending on the biomass origin.
29492107	0	73	theme	recalcitrance	95:107	arg1	markers					69:75	generic markers	61:75	generic markers of lignocellulose recalcitrance	61:107	Multimodal analysis of pretreated biomass species highlights generic markers of lignocellulose recalcitrance.
29492107	5	74	theme	initial	912:918	arg1	content					928:934	the initial glucose content	908:934	the initial glucose content released after 96 h of saccharification	908:974	RESULTS The pretreatment conditions selected allowed obtaining samples displaying different susceptibility to enzymatic hydrolysis (from 3 up to 98% of the initial glucose content released after 96 h of saccharification).
29492107	6	75	theme	measured	1039:1046	arg1	features					1072:1079	the measured chemical and structural features	1035:1079	the measured chemical and structural features	1035:1079	Generic correlation coefficients were calculated between the measured chemical and structural features and the final saccharification rates.
29492107	13	76	theme	Lignin	2068:2073	arg1	composition					2075:2085	Lignin composition	2068:2085	Lignin composition	2068:2085	Lignin composition, which probably influences its structure, is the most important parameter to overcome to enhance enzymes access to the polysaccharides.
29492107	13	76	theme	Lignin	2068:2073	arg1	parameter					2151:2159	the most important parameter	2132:2159	the most important parameter to overcome to enhance enzymes access to the polysaccharides	2132:2220	Lignin composition, which probably influences its structure, is the most important parameter to overcome to enhance enzymes access to the polysaccharides.
29492107	7	77	theme	considered	1284:1293	arg1	biomass					1295:1301	the considered biomass	1280:1301	the considered biomass	1280:1301	Increases in porosity displayed overall strong positive correlations with saccharification efficiency, but different porosity ranges were concerned depending on the considered biomass.
29492107	10	78	from	monolignols	1664:1674	arg1	tissues					1686:1692	mature tissues	1679:1692	mature tissues	1679:1692	Lignin influence was highlighted by the strong negative correlation with fluorescence intensity which mainly originates from monolignols in mature tissues.
29492107	3	79	theme	standard	394:401	arg1	pretreatments					403:415	four standard pretreatments	389:415	four standard pretreatments (dilute acid, hot water, ionic liquid and sodium chlorite)	389:474	Three representative biomass species (wheat straw, poplar and miscanthus) were submitted to four standard pretreatments (dilute acid, hot water, ionic liquid and sodium chlorite) in order to generate a set of contrasted samples.
29492107	3	79	theme	standard	394:401	arg1	acid					425:428	dilute acid	418:428	dilute acid	418:428	Three representative biomass species (wheat straw, poplar and miscanthus) were submitted to four standard pretreatments (dilute acid, hot water, ionic liquid and sodium chlorite) in order to generate a set of contrasted samples.
29492107	3	79	theme	standard	394:401	arg1	water					435:439	hot water	431:439	hot water	431:439	Three representative biomass species (wheat straw, poplar and miscanthus) were submitted to four standard pretreatments (dilute acid, hot water, ionic liquid and sodium chlorite) in order to generate a set of contrasted samples.
29492107	3	79	theme	standard	394:401	arg1	chlorite					466:473	sodium chlorite	459:473	sodium chlorite	459:473	Three representative biomass species (wheat straw, poplar and miscanthus) were submitted to four standard pretreatments (dilute acid, hot water, ionic liquid and sodium chlorite) in order to generate a set of contrasted samples.
29492107	10	80	theme	negative	1586:1593	arg1	correlation					1595:1605	the strong negative correlation	1575:1605	the strong negative correlation with fluorescence intensity which mainly originates from monolignols in mature tissues	1575:1692	Lignin influence was highlighted by the strong negative correlation with fluorescence intensity which mainly originates from monolignols in mature tissues.
29492107	3	81	theme	sodium	459:464	arg1	pretreatments					403:415	four standard pretreatments	389:415	four standard pretreatments (dilute acid, hot water, ionic liquid and sodium chlorite)	389:474	Three representative biomass species (wheat straw, poplar and miscanthus) were submitted to four standard pretreatments (dilute acid, hot water, ionic liquid and sodium chlorite) in order to generate a set of contrasted samples.
29492107	3	81	theme	sodium	459:464	arg1	chlorite					466:473	sodium chlorite	459:473	sodium chlorite	459:473	Three representative biomass species (wheat straw, poplar and miscanthus) were submitted to four standard pretreatments (dilute acid, hot water, ionic liquid and sodium chlorite) in order to generate a set of contrasted samples.
29492107	5	82	theme	different	838:846	arg1	susceptibility					848:861	different susceptibility	838:861	different susceptibility to enzymatic hydrolysis (from 3 up to 98% of the initial glucose content released after 96 h of saccharification)	838:975	RESULTS The pretreatment conditions selected allowed obtaining samples displaying different susceptibility to enzymatic hydrolysis (from 3 up to 98% of the initial glucose content released after 96 h of saccharification).
29492107	12	83	with	correlations	1853:1864	arg1	ranges					1889:1894	specific porosity ranges	1871:1894	specific porosity ranges	1871:1894	The correlations with specific porosity ranges are biomass species-dependent, meaning that enzymes cocktails with fitted enzyme size are likely to be needed to optimise saccharification depending on the biomass origin.
29492107	4	84	theme	biomass	733:739	arg1	recalcitrance					741:753	biomass recalcitrance	733:753	biomass recalcitrance	733:753	A large array of techniques, including wet chemistry analysis, porosity measurements using NMR spectroscopy, electron and fluorescence microscopy, were used in order to determine possible generic factors of biomass recalcitrance.
29492107	0	85	theme	pretreated	23:32	arg1	species					42:48	pretreated biomass species	23:48	pretreated biomass species	23:48	Multimodal analysis of pretreated biomass species highlights generic markers of lignocellulose recalcitrance.
29492107	1	86	theme	Biomass	121:127	arg1	recalcitrance					129:141	BACKGROUND Biomass recalcitrance	110:141	BACKGROUND Biomass recalcitrance to enzymatic hydrolysis	110:165	BACKGROUND Biomass recalcitrance to enzymatic hydrolysis has been assigned to several structural and chemical factors.
29492107	6	87	theme	saccharification	1095:1110	arg1	rates					1112:1116	the final saccharification rates	1085:1116	the final saccharification rates	1085:1116	Generic correlation coefficients were calculated between the measured chemical and structural features and the final saccharification rates.
29492107	4	88	theme	wet	565:567	arg1	analysis					579:586	wet chemistry analysis	565:586	wet chemistry analysis	565:586	A large array of techniques, including wet chemistry analysis, porosity measurements using NMR spectroscopy, electron and fluorescence microscopy, were used in order to determine possible generic factors of biomass recalcitrance.
29492107	0	89	theme	species	42:48	arg1	analysis					11:18	Multimodal analysis	0:18	Multimodal analysis of pretreated biomass species	0:48	Multimodal analysis of pretreated biomass species highlights generic markers of lignocellulose recalcitrance.
29492107	12	90	theme	biomass	2052:2058	arg1	origin					2060:2065	the biomass origin	2048:2065	the biomass origin	2048:2065	The correlations with specific porosity ranges are biomass species-dependent, meaning that enzymes cocktails with fitted enzyme size are likely to be needed to optimise saccharification depending on the biomass origin.
29492107	4	91	theme	large	528:532	arg1	array					534:538	A large array	526:538	A large array of techniques, including wet chemistry analysis, porosity measurements using NMR spectroscopy, electron and fluorescence microscopy,	526:671	A large array of techniques, including wet chemistry analysis, porosity measurements using NMR spectroscopy, electron and fluorescence microscopy, were used in order to determine possible generic factors of biomass recalcitrance.
29492107	14	92	theme	fluorescence	2236:2247	arg1	intensity					2249:2257	fluorescence intensity	2236:2257	fluorescence intensity	2236:2257	Accordingly, fluorescence intensity was found to be a rapid and simple method to assess recalcitrance after pretreatment.
29492107	14	92	theme	fluorescence	2236:2247	arg1	method					2294:2299	a rapid and simple method	2275:2299	a rapid and simple method to assess recalcitrance after pretreatment	2275:2342	Accordingly, fluorescence intensity was found to be a rapid and simple method to assess recalcitrance after pretreatment.
29492107	13	93	theme	enzymes	2184:2190	arg1	access					2192:2197	enzymes access	2184:2197	enzymes access to the polysaccharides	2184:2220	Lignin composition, which probably influences its structure, is the most important parameter to overcome to enhance enzymes access to the polysaccharides.
29492107	0	94	theme	generic	61:67	arg1	markers					69:75	generic markers	61:75	generic markers of lignocellulose recalcitrance	61:107	Multimodal analysis of pretreated biomass species highlights generic markers of lignocellulose recalcitrance.
29492107	3	95	theme	hot	431:433	arg1	pretreatments					403:415	four standard pretreatments	389:415	four standard pretreatments (dilute acid, hot water, ionic liquid and sodium chlorite)	389:474	Three representative biomass species (wheat straw, poplar and miscanthus) were submitted to four standard pretreatments (dilute acid, hot water, ionic liquid and sodium chlorite) in order to generate a set of contrasted samples.
29492107	3	95	theme	hot	431:433	arg1	water					435:439	hot water	431:439	hot water	431:439	Three representative biomass species (wheat straw, poplar and miscanthus) were submitted to four standard pretreatments (dilute acid, hot water, ionic liquid and sodium chlorite) in order to generate a set of contrasted samples.
29492107	11	96	theme	responsible	1765:1775	arg1	factors					1757:1763	the factors	1753:1763	the factors responsible for biomass recalcitrance that can reasonably be considered as generic	1753:1846	CONCLUSIONS Our results provide a better understanding of the factors responsible for biomass recalcitrance that can reasonably be considered as generic.
29492107	9	97	theme	enzymatic	1493:1501	arg1	hydrolysis					1503:1512	enzymatic hydrolysis	1493:1512	enzymatic hydrolysis than lignin composition	1493:1536	Lignin content, which is likely involved in the correlations observed for porosity, was less detrimental to enzymatic hydrolysis than lignin composition.
29901065	1	0	theme	Synthesis	151:159	arg1	strategy					169:176	A new and efficient Ionic Liquid-Supported Oligosaccharide Synthesis (ILSOS) strategy	92:176	A new and efficient Ionic Liquid-Supported Oligosaccharide Synthesis (ILSOS) strategy for an N-linked core pentasaccharide on a 10 g scale	92:229	A new and efficient Ionic Liquid-Supported Oligosaccharide Synthesis (ILSOS) strategy for an N-linked core pentasaccharide on a 10 g scale is reported.
29901065	2	1	theme	core	419:422	arg1	pentasaccharide					424:438	the N-linked core pentasaccharide	406:438	the N-linked core pentasaccharide	406:438	This new ILSOS includes a new spacer for an IL support, a new tagging strategy, and fast, efficient and orthogonal removal of the ionic-liquid support, producing the N-linked core pentasaccharide with direct applicability potential in a short time, with high yield and on a large gram scale.
29901065	2	2	theme	gram	524:527	arg1	scale					529:533	a large gram scale	516:533	a large gram scale	516:533	This new ILSOS includes a new spacer for an IL support, a new tagging strategy, and fast, efficient and orthogonal removal of the ionic-liquid support, producing the N-linked core pentasaccharide with direct applicability potential in a short time, with high yield and on a large gram scale.
29901065	2	3	theme	large	518:522	arg1	scale					529:533	a large gram scale	516:533	a large gram scale	516:533	This new ILSOS includes a new spacer for an IL support, a new tagging strategy, and fast, efficient and orthogonal removal of the ionic-liquid support, producing the N-linked core pentasaccharide with direct applicability potential in a short time, with high yield and on a large gram scale.
29901065	0	4	theme	g	83:83	arg1	scale					85:89	a 10 g scale	78:89	a 10 g scale	78:89	Ionic-liquid supported rapid synthesis of an N-glycan core pentasaccharide on a 10 g scale.
29901065	1	5	link	N-linked	185:192	arg1	pentasaccharide					199:213	an N-linked core pentasaccharide	182:213	an N-linked core pentasaccharide	182:213	A new and efficient Ionic Liquid-Supported Oligosaccharide Synthesis (ILSOS) strategy for an N-linked core pentasaccharide on a 10 g scale is reported.
29901065	2	6	theme	new	302:304	arg1	strategy					314:321	a new tagging strategy	300:321	a new tagging strategy	300:321	This new ILSOS includes a new spacer for an IL support, a new tagging strategy, and fast, efficient and orthogonal removal of the ionic-liquid support, producing the N-linked core pentasaccharide with direct applicability potential in a short time, with high yield and on a large gram scale.
29901065	2	7	theme	orthogonal	348:357	arg1	removal					359:365	fast, efficient and orthogonal removal	328:365	fast, efficient and orthogonal removal of the ionic-liquid support	328:393	This new ILSOS includes a new spacer for an IL support, a new tagging strategy, and fast, efficient and orthogonal removal of the ionic-liquid support, producing the N-linked core pentasaccharide with direct applicability potential in a short time, with high yield and on a large gram scale.
29901065	2	8	theme	new	249:251	arg1	ILSOS					253:257	This new ILSOS	244:257	This new ILSOS	244:257	This new ILSOS includes a new spacer for an IL support, a new tagging strategy, and fast, efficient and orthogonal removal of the ionic-liquid support, producing the N-linked core pentasaccharide with direct applicability potential in a short time, with high yield and on a large gram scale.
29901065	1	9	theme	ILSOS	162:166	arg1	strategy					169:176	A new and efficient Ionic Liquid-Supported Oligosaccharide Synthesis (ILSOS) strategy	92:176	A new and efficient Ionic Liquid-Supported Oligosaccharide Synthesis (ILSOS) strategy for an N-linked core pentasaccharide on a 10 g scale	92:229	A new and efficient Ionic Liquid-Supported Oligosaccharide Synthesis (ILSOS) strategy for an N-linked core pentasaccharide on a 10 g scale is reported.
29901065	2	10	theme	applicability	452:464	arg1	potential					466:474	direct applicability potential	445:474	direct applicability potential in a short time	445:490	This new ILSOS includes a new spacer for an IL support, a new tagging strategy, and fast, efficient and orthogonal removal of the ionic-liquid support, producing the N-linked core pentasaccharide with direct applicability potential in a short time, with high yield and on a large gram scale.
29901065	2	11	theme	fast	328:331	arg1	removal					359:365	fast, efficient and orthogonal removal	328:365	fast, efficient and orthogonal removal of the ionic-liquid support	328:393	This new ILSOS includes a new spacer for an IL support, a new tagging strategy, and fast, efficient and orthogonal removal of the ionic-liquid support, producing the N-linked core pentasaccharide with direct applicability potential in a short time, with high yield and on a large gram scale.
29901065	2	12	theme	support	387:393	arg1	support					291:297	an IL support	285:297	an IL support	285:297	This new ILSOS includes a new spacer for an IL support, a new tagging strategy, and fast, efficient and orthogonal removal of the ionic-liquid support, producing the N-linked core pentasaccharide with direct applicability potential in a short time, with high yield and on a large gram scale.
29901065	2	12	theme	support	387:393	arg1	strategy					314:321	a new tagging strategy	300:321	a new tagging strategy	300:321	This new ILSOS includes a new spacer for an IL support, a new tagging strategy, and fast, efficient and orthogonal removal of the ionic-liquid support, producing the N-linked core pentasaccharide with direct applicability potential in a short time, with high yield and on a large gram scale.
29901065	2	12	theme	support	387:393	arg1	removal					359:365	fast, efficient and orthogonal removal	328:365	fast, efficient and orthogonal removal of the ionic-liquid support	328:393	This new ILSOS includes a new spacer for an IL support, a new tagging strategy, and fast, efficient and orthogonal removal of the ionic-liquid support, producing the N-linked core pentasaccharide with direct applicability potential in a short time, with high yield and on a large gram scale.
29901065	2	13	theme	ionic-liquid	374:385	arg1	support					387:393	the ionic-liquid support	370:393	the ionic-liquid support	370:393	This new ILSOS includes a new spacer for an IL support, a new tagging strategy, and fast, efficient and orthogonal removal of the ionic-liquid support, producing the N-linked core pentasaccharide with direct applicability potential in a short time, with high yield and on a large gram scale.
29901065	2	14	link	N-linked	410:417	arg1	pentasaccharide					424:438	the N-linked core pentasaccharide	406:438	the N-linked core pentasaccharide	406:438	This new ILSOS includes a new spacer for an IL support, a new tagging strategy, and fast, efficient and orthogonal removal of the ionic-liquid support, producing the N-linked core pentasaccharide with direct applicability potential in a short time, with high yield and on a large gram scale.
29901065	2	15	theme	tagging	306:312	arg1	strategy					314:321	a new tagging strategy	300:321	a new tagging strategy	300:321	This new ILSOS includes a new spacer for an IL support, a new tagging strategy, and fast, efficient and orthogonal removal of the ionic-liquid support, producing the N-linked core pentasaccharide with direct applicability potential in a short time, with high yield and on a large gram scale.
29901065	2	16	theme	new	270:272	arg1	spacer					274:279	a new spacer	268:279	a new spacer for an IL support, a new tagging strategy, and fast, efficient and orthogonal removal of the ionic-liquid support	268:393	This new ILSOS includes a new spacer for an IL support, a new tagging strategy, and fast, efficient and orthogonal removal of the ionic-liquid support, producing the N-linked core pentasaccharide with direct applicability potential in a short time, with high yield and on a large gram scale.
29901065	0	17	theme	rapid	23:27	arg1	synthesis					29:37	rapid synthesis	23:37	rapid synthesis of an N-glycan core pentasaccharide	23:73	Ionic-liquid supported rapid synthesis of an N-glycan core pentasaccharide on a 10 g scale.
29901065	1	18	theme	N-linked	185:192	arg1	pentasaccharide					199:213	an N-linked core pentasaccharide	182:213	an N-linked core pentasaccharide	182:213	A new and efficient Ionic Liquid-Supported Oligosaccharide Synthesis (ILSOS) strategy for an N-linked core pentasaccharide on a 10 g scale is reported.
29901065	2	19	theme	N-linked	410:417	arg1	pentasaccharide					424:438	the N-linked core pentasaccharide	406:438	the N-linked core pentasaccharide	406:438	This new ILSOS includes a new spacer for an IL support, a new tagging strategy, and fast, efficient and orthogonal removal of the ionic-liquid support, producing the N-linked core pentasaccharide with direct applicability potential in a short time, with high yield and on a large gram scale.
29901065	1	20	theme	core	194:197	arg1	pentasaccharide					199:213	an N-linked core pentasaccharide	182:213	an N-linked core pentasaccharide	182:213	A new and efficient Ionic Liquid-Supported Oligosaccharide Synthesis (ILSOS) strategy for an N-linked core pentasaccharide on a 10 g scale is reported.
29901065	2	21	theme	short	481:485	arg1	time					487:490	a short time	479:490	a short time	479:490	This new ILSOS includes a new spacer for an IL support, a new tagging strategy, and fast, efficient and orthogonal removal of the ionic-liquid support, producing the N-linked core pentasaccharide with direct applicability potential in a short time, with high yield and on a large gram scale.
29901065	2	22	theme	efficient	334:342	arg1	removal					359:365	fast, efficient and orthogonal removal	328:365	fast, efficient and orthogonal removal of the ionic-liquid support	328:393	This new ILSOS includes a new spacer for an IL support, a new tagging strategy, and fast, efficient and orthogonal removal of the ionic-liquid support, producing the N-linked core pentasaccharide with direct applicability potential in a short time, with high yield and on a large gram scale.
29901065	0	23	theme	core	54:57	arg1	pentasaccharide					59:73	an N-glycan core pentasaccharide	42:73	an N-glycan core pentasaccharide	42:73	Ionic-liquid supported rapid synthesis of an N-glycan core pentasaccharide on a 10 g scale.
29901065	1	24	theme	new	94:96	arg1	strategy					169:176	A new and efficient Ionic Liquid-Supported Oligosaccharide Synthesis (ILSOS) strategy	92:176	A new and efficient Ionic Liquid-Supported Oligosaccharide Synthesis (ILSOS) strategy for an N-linked core pentasaccharide on a 10 g scale	92:229	A new and efficient Ionic Liquid-Supported Oligosaccharide Synthesis (ILSOS) strategy for an N-linked core pentasaccharide on a 10 g scale is reported.
29901065	0	25	theme	N-glycan	45:52	arg1	pentasaccharide					59:73	an N-glycan core pentasaccharide	42:73	an N-glycan core pentasaccharide	42:73	Ionic-liquid supported rapid synthesis of an N-glycan core pentasaccharide on a 10 g scale.
29901065	2	26	from	potential	466:474	arg1	time					487:490	a short time	479:490	a short time	479:490	This new ILSOS includes a new spacer for an IL support, a new tagging strategy, and fast, efficient and orthogonal removal of the ionic-liquid support, producing the N-linked core pentasaccharide with direct applicability potential in a short time, with high yield and on a large gram scale.
29901065	1	27	theme	efficient	102:110	arg1	strategy					169:176	A new and efficient Ionic Liquid-Supported Oligosaccharide Synthesis (ILSOS) strategy	92:176	A new and efficient Ionic Liquid-Supported Oligosaccharide Synthesis (ILSOS) strategy for an N-linked core pentasaccharide on a 10 g scale	92:229	A new and efficient Ionic Liquid-Supported Oligosaccharide Synthesis (ILSOS) strategy for an N-linked core pentasaccharide on a 10 g scale is reported.
29901065	2	28	theme	high	498:501	arg1	yield					503:507	high yield	498:507	high yield	498:507	This new ILSOS includes a new spacer for an IL support, a new tagging strategy, and fast, efficient and orthogonal removal of the ionic-liquid support, producing the N-linked core pentasaccharide with direct applicability potential in a short time, with high yield and on a large gram scale.
29901065	1	29	from	strategy	169:176	arg1	scale					225:229	a 10 g scale	218:229	a 10 g scale	218:229	A new and efficient Ionic Liquid-Supported Oligosaccharide Synthesis (ILSOS) strategy for an N-linked core pentasaccharide on a 10 g scale is reported.
29901065	1	30	theme	Ionic	112:116	arg1	strategy					169:176	A new and efficient Ionic Liquid-Supported Oligosaccharide Synthesis (ILSOS) strategy	92:176	A new and efficient Ionic Liquid-Supported Oligosaccharide Synthesis (ILSOS) strategy for an N-linked core pentasaccharide on a 10 g scale	92:229	A new and efficient Ionic Liquid-Supported Oligosaccharide Synthesis (ILSOS) strategy for an N-linked core pentasaccharide on a 10 g scale is reported.
29901065	2	31	theme	IL	288:289	arg1	support					291:297	an IL support	285:297	an IL support	285:297	This new ILSOS includes a new spacer for an IL support, a new tagging strategy, and fast, efficient and orthogonal removal of the ionic-liquid support, producing the N-linked core pentasaccharide with direct applicability potential in a short time, with high yield and on a large gram scale.
29901065	2	32	theme	direct	445:450	arg1	potential					466:474	direct applicability potential	445:474	direct applicability potential in a short time	445:490	This new ILSOS includes a new spacer for an IL support, a new tagging strategy, and fast, efficient and orthogonal removal of the ionic-liquid support, producing the N-linked core pentasaccharide with direct applicability potential in a short time, with high yield and on a large gram scale.
29901065	1	33	theme	Liquid-Supported	118:133	arg1	strategy					169:176	A new and efficient Ionic Liquid-Supported Oligosaccharide Synthesis (ILSOS) strategy	92:176	A new and efficient Ionic Liquid-Supported Oligosaccharide Synthesis (ILSOS) strategy for an N-linked core pentasaccharide on a 10 g scale	92:229	A new and efficient Ionic Liquid-Supported Oligosaccharide Synthesis (ILSOS) strategy for an N-linked core pentasaccharide on a 10 g scale is reported.
29901065	0	34	theme	pentasaccharide	59:73	arg1	synthesis					29:37	rapid synthesis	23:37	rapid synthesis of an N-glycan core pentasaccharide	23:73	Ionic-liquid supported rapid synthesis of an N-glycan core pentasaccharide on a 10 g scale.
29901065	1	35	theme	Oligosaccharide	135:149	arg1	strategy					169:176	A new and efficient Ionic Liquid-Supported Oligosaccharide Synthesis (ILSOS) strategy	92:176	A new and efficient Ionic Liquid-Supported Oligosaccharide Synthesis (ILSOS) strategy for an N-linked core pentasaccharide on a 10 g scale	92:229	A new and efficient Ionic Liquid-Supported Oligosaccharide Synthesis (ILSOS) strategy for an N-linked core pentasaccharide on a 10 g scale is reported.
29901065	1	36	theme	g	223:223	arg1	scale					225:229	a 10 g scale	218:229	a 10 g scale	218:229	A new and efficient Ionic Liquid-Supported Oligosaccharide Synthesis (ILSOS) strategy for an N-linked core pentasaccharide on a 10 g scale is reported.
29687353	7	0	theme	glycan	1381:1386	arg1	biosynthesis					1388:1399	glycan biosynthesis	1381:1399	glycan biosynthesis	1381:1399	Predicted functional information showed that bacterial motility proteins, flagellar assembly, and fatty acid biosynthesis metabolism were significantly overrepresented in cancer patients, while amino acid metabolism and glycan biosynthesis were overrepresented in controls.
29687353	2	1	theme	patients	309:316	arg1	microbiome					279:288	the stool microbiome	269:288	the stool microbiome of Moroccan cancer patients with healthy individuals	269:341	The aim of our study was to compare the stool microbiome of Moroccan cancer patients with healthy individuals.
29687353	6	2	theme	other	1146:1150	arg1	studies					1152:1158	other studies	1146:1158	other studies	1146:1158	Porphyromonas, Clostridium, Ruminococcus, Selenomonas, and Fusobacterium were significantly overrepresented in diseased patients, similarly to other studies.
29687353	8	3	theme	independent	1553:1563	arg1	process					1544:1550	the carcinogenesis process	1525:1550	the carcinogenesis process	1525:1550	This suggests that involvement of these functional metagenomes is similar and relevant in the carcinogenesis process, independent of the origin of the samples.
29687353	2	4	theme	study	248:252	arg1	aim					237:239	The aim	233:239	The aim of our study	233:252	The aim of our study was to compare the stool microbiome of Moroccan cancer patients with healthy individuals.
29687353	7	5	theme	amino	1355:1359	arg1	metabolism					1366:1375	amino acid metabolism	1355:1375	amino acid metabolism	1355:1375	Predicted functional information showed that bacterial motility proteins, flagellar assembly, and fatty acid biosynthesis metabolism were significantly overrepresented in cancer patients, while amino acid metabolism and glycan biosynthesis were overrepresented in controls.
29687353	5	6	dep	%	909:909	arg1	p = 0.06					923:930	p = 0.06	923:930	p = 0.06	923:930	Our findings showed that cancer samples had higher proportions of Firmicutes (T = 50.5%; N = 28.4%; p = 0.04), specifically of Clostridia (T = 48.3%; N = 19.0%; p = 0.002), and Fusobacteria (T = 0.1%; N = 0.0%; p = 0.02), especially of Fusobacteriia (T = 0.1%; N = 0.0%; p = 0.02), while Bacteroidetes were enriched in healthy samples (T = 35.1%; N = 62.8%; p = 0.06), particularly the class Bacteroidia (T = 35.1%; N = 62.6%; p = 0.06).
29687353	5	6	dep	%	909:909	arg1	%					920:920	N = 62.8%	912:920	T = 35.1%; N = 62.8%; p = 0.06	901:930	Our findings showed that cancer samples had higher proportions of Firmicutes (T = 50.5%; N = 28.4%; p = 0.04), specifically of Clostridia (T = 48.3%; N = 19.0%; p = 0.002), and Fusobacteria (T = 0.1%; N = 0.0%; p = 0.02), especially of Fusobacteriia (T = 0.1%; N = 0.0%; p = 0.02), while Bacteroidetes were enriched in healthy samples (T = 35.1%; N = 62.8%; p = 0.06), particularly the class Bacteroidia (T = 35.1%; N = 62.6%; p = 0.06).
29687353	6	7	theme	diseased	1114:1121	arg1	patients					1123:1130	diseased patients	1114:1130	diseased patients	1114:1130	Porphyromonas, Clostridium, Ruminococcus, Selenomonas, and Fusobacterium were significantly overrepresented in diseased patients, similarly to other studies.
29687353	0	8	theme	Gut	0:2	arg1	microbiome					4:13	Gut microbiome	0:13	Gut microbiome of Moroccan colorectal cancer patients.	0:53	Gut microbiome of Moroccan colorectal cancer patients.
29687353	1	9	theme	microbiome	158:167	arg1	changes					169:175	the microbiome changes	154:175	the microbiome changes associated with the disease in the Moroccan population	154:230	Although colorectal cancer is the third leading cause of death in Morocco, there are no studies of the microbiome changes associated with the disease in the Moroccan population.
29687353	7	10	theme	acid	1265:1268	arg1	metabolism					1283:1292	fatty acid biosynthesis metabolism	1259:1292	fatty acid biosynthesis metabolism	1259:1292	Predicted functional information showed that bacterial motility proteins, flagellar assembly, and fatty acid biosynthesis metabolism were significantly overrepresented in cancer patients, while amino acid metabolism and glycan biosynthesis were overrepresented in controls.
29687353	5	11	dep	%	712:712	arg1	p = 0.002					726:734	p = 0.002	726:734	p = 0.002	726:734	Our findings showed that cancer samples had higher proportions of Firmicutes (T = 50.5%; N = 28.4%; p = 0.04), specifically of Clostridia (T = 48.3%; N = 19.0%; p = 0.002), and Fusobacteria (T = 0.1%; N = 0.0%; p = 0.02), especially of Fusobacteriia (T = 0.1%; N = 0.0%; p = 0.02), while Bacteroidetes were enriched in healthy samples (T = 35.1%; N = 62.8%; p = 0.06), particularly the class Bacteroidia (T = 35.1%; N = 62.6%; p = 0.06).
29687353	5	11	dep	%	712:712	arg1	%					723:723	N = 19.0%	715:723	T = 48.3%; N = 19.0%; p = 0.002	704:734	Our findings showed that cancer samples had higher proportions of Firmicutes (T = 50.5%; N = 28.4%; p = 0.04), specifically of Clostridia (T = 48.3%; N = 19.0%; p = 0.002), and Fusobacteria (T = 0.1%; N = 0.0%; p = 0.02), especially of Fusobacteriia (T = 0.1%; N = 0.0%; p = 0.02), while Bacteroidetes were enriched in healthy samples (T = 35.1%; N = 62.8%; p = 0.06), particularly the class Bacteroidia (T = 35.1%; N = 62.6%; p = 0.06).
29687353	1	12	from	disease	197:203	arg1	population					221:230	the Moroccan population	208:230	the Moroccan population	208:230	Although colorectal cancer is the third leading cause of death in Morocco, there are no studies of the microbiome changes associated with the disease in the Moroccan population.
29687353	1	13	theme	death	112:116	arg1	cancer					75:80	colorectal cancer	64:80	colorectal cancer	64:80	Although colorectal cancer is the third leading cause of death in Morocco, there are no studies of the microbiome changes associated with the disease in the Moroccan population.
29687353	1	13	theme	death	112:116	arg1	cause					103:107	the third leading cause	85:107	the third leading cause of death in Morocco	85:127	Although colorectal cancer is the third leading cause of death in Morocco, there are no studies of the microbiome changes associated with the disease in the Moroccan population.
29687353	9	14	theme	bacterial	1645:1653	arg1	taxa					1655:1658	bacterial taxa	1645:1658	bacterial taxa relevant to the Moroccan population	1645:1694	Results from this study allowed identification of bacterial taxa relevant to the Moroccan population and encourages larger studies to facilitate population-directed therapeutic approaches.
29687353	9	15	theme	relevant	1660:1667	arg1	taxa					1655:1658	bacterial taxa	1645:1658	bacterial taxa relevant to the Moroccan population	1645:1694	Results from this study allowed identification of bacterial taxa relevant to the Moroccan population and encourages larger studies to facilitate population-directed therapeutic approaches.
29687353	7	16	theme	motility	1216:1223	arg1	proteins					1225:1232	bacterial motility proteins	1206:1232	bacterial motility proteins	1206:1232	Predicted functional information showed that bacterial motility proteins, flagellar assembly, and fatty acid biosynthesis metabolism were significantly overrepresented in cancer patients, while amino acid metabolism and glycan biosynthesis were overrepresented in controls.
29687353	5	17	dep	%	978:978	arg1	p = 0.06					992:999	p = 0.06	992:999	p = 0.06	992:999	Our findings showed that cancer samples had higher proportions of Firmicutes (T = 50.5%; N = 28.4%; p = 0.04), specifically of Clostridia (T = 48.3%; N = 19.0%; p = 0.002), and Fusobacteria (T = 0.1%; N = 0.0%; p = 0.02), especially of Fusobacteriia (T = 0.1%; N = 0.0%; p = 0.02), while Bacteroidetes were enriched in healthy samples (T = 35.1%; N = 62.8%; p = 0.06), particularly the class Bacteroidia (T = 35.1%; N = 62.6%; p = 0.06).
29687353	5	17	dep	%	978:978	arg1	%					989:989	N = 62.6%	981:989	T = 35.1%; N = 62.6%; p = 0.06	970:999	Our findings showed that cancer samples had higher proportions of Firmicutes (T = 50.5%; N = 28.4%; p = 0.04), specifically of Clostridia (T = 48.3%; N = 19.0%; p = 0.002), and Fusobacteria (T = 0.1%; N = 0.0%; p = 0.02), especially of Fusobacteriia (T = 0.1%; N = 0.0%; p = 0.02), while Bacteroidetes were enriched in healthy samples (T = 35.1%; N = 62.8%; p = 0.06), particularly the class Bacteroidia (T = 35.1%; N = 62.6%; p = 0.06).
29687353	5	18	dep	Clostridia	692:701	arg1	%					712:712	T = 48.3%	704:712	T = 48.3%; N = 19.0%; p = 0.002	704:734	Our findings showed that cancer samples had higher proportions of Firmicutes (T = 50.5%; N = 28.4%; p = 0.04), specifically of Clostridia (T = 48.3%; N = 19.0%; p = 0.002), and Fusobacteria (T = 0.1%; N = 0.0%; p = 0.02), especially of Fusobacteriia (T = 0.1%; N = 0.0%; p = 0.02), while Bacteroidetes were enriched in healthy samples (T = 35.1%; N = 62.8%; p = 0.06), particularly the class Bacteroidia (T = 35.1%; N = 62.6%; p = 0.06).
29687353	4	19	theme	Principal	475:483	arg1	analysis					496:503	Principal coordinate analysis	475:503	Principal coordinate analysis of samples	475:514	Principal coordinate analysis of samples revealed defined cancer versus healthy clusters.
29687353	5	20	theme	Clostridia	692:701	arg1	proportions					616:626	higher proportions	609:626	higher proportions	609:626	Our findings showed that cancer samples had higher proportions of Firmicutes (T = 50.5%; N = 28.4%; p = 0.04), specifically of Clostridia (T = 48.3%; N = 19.0%; p = 0.002), and Fusobacteria (T = 0.1%; N = 0.0%; p = 0.02), especially of Fusobacteriia (T = 0.1%; N = 0.0%; p = 0.02), while Bacteroidetes were enriched in healthy samples (T = 35.1%; N = 62.8%; p = 0.06), particularly the class Bacteroidia (T = 35.1%; N = 62.6%; p = 0.06).
29687353	5	21	theme	class	951:955	arg1	samples					892:898	healthy samples	884:898	healthy samples (T = 35.1%; N = 62.8%; p = 0.06)	884:931	Our findings showed that cancer samples had higher proportions of Firmicutes (T = 50.5%; N = 28.4%; p = 0.04), specifically of Clostridia (T = 48.3%; N = 19.0%; p = 0.002), and Fusobacteria (T = 0.1%; N = 0.0%; p = 0.02), especially of Fusobacteriia (T = 0.1%; N = 0.0%; p = 0.02), while Bacteroidetes were enriched in healthy samples (T = 35.1%; N = 62.8%; p = 0.06), particularly the class Bacteroidia (T = 35.1%; N = 62.6%; p = 0.06).
29687353	5	21	theme	class	951:955	arg1	Bacteroidia					957:967	the class Bacteroidia	947:967	the class Bacteroidia (T = 35.1%; N = 62.6%; p = 0.06)	947:1000	Our findings showed that cancer samples had higher proportions of Firmicutes (T = 50.5%; N = 28.4%; p = 0.04), specifically of Clostridia (T = 48.3%; N = 19.0%; p = 0.002), and Fusobacteria (T = 0.1%; N = 0.0%; p = 0.02), especially of Fusobacteriia (T = 0.1%; N = 0.0%; p = 0.02), while Bacteroidetes were enriched in healthy samples (T = 35.1%; N = 62.8%; p = 0.06), particularly the class Bacteroidia (T = 35.1%; N = 62.6%; p = 0.06).
29687353	5	22	theme	cancer	590:595	arg1	samples					597:603	cancer samples	590:603	cancer samples	590:603	Our findings showed that cancer samples had higher proportions of Firmicutes (T = 50.5%; N = 28.4%; p = 0.04), specifically of Clostridia (T = 48.3%; N = 19.0%; p = 0.002), and Fusobacteria (T = 0.1%; N = 0.0%; p = 0.02), especially of Fusobacteriia (T = 0.1%; N = 0.0%; p = 0.02), while Bacteroidetes were enriched in healthy samples (T = 35.1%; N = 62.8%; p = 0.06), particularly the class Bacteroidia (T = 35.1%; N = 62.6%; p = 0.06).
29687353	1	23	theme	colorectal	64:73	arg1	cancer					75:80	colorectal cancer	64:80	colorectal cancer	64:80	Although colorectal cancer is the third leading cause of death in Morocco, there are no studies of the microbiome changes associated with the disease in the Moroccan population.
29687353	1	23	theme	colorectal	64:73	arg1	cause					103:107	the third leading cause	85:107	the third leading cause of death in Morocco	85:127	Although colorectal cancer is the third leading cause of death in Morocco, there are no studies of the microbiome changes associated with the disease in the Moroccan population.
29687353	3	24	theme	CRC	402:404	arg1	patients					406:413	11 CRC patients	399:413	11 CRC patients	399:413	We analyzed the microbiome composition of samples from 11 CRC patients and 12 healthy individuals by 16S rRNA amplicon sequencing.
29687353	7	25	theme	bacterial	1206:1214	arg1	proteins					1225:1232	bacterial motility proteins	1206:1232	bacterial motility proteins	1206:1232	Predicted functional information showed that bacterial motility proteins, flagellar assembly, and fatty acid biosynthesis metabolism were significantly overrepresented in cancer patients, while amino acid metabolism and glycan biosynthesis were overrepresented in controls.
29687353	5	26	dep	Fusobacteriia	801:813	arg1	%					823:823	T = 0.1%	816:823	T = 0.1%; N = 0.0%; p = 0.02	816:843	Our findings showed that cancer samples had higher proportions of Firmicutes (T = 50.5%; N = 28.4%; p = 0.04), specifically of Clostridia (T = 48.3%; N = 19.0%; p = 0.002), and Fusobacteria (T = 0.1%; N = 0.0%; p = 0.02), especially of Fusobacteriia (T = 0.1%; N = 0.0%; p = 0.02), while Bacteroidetes were enriched in healthy samples (T = 35.1%; N = 62.8%; p = 0.06), particularly the class Bacteroidia (T = 35.1%; N = 62.6%; p = 0.06).
29687353	5	27	theme	Fusobacteria	742:753	arg1	proportions					616:626	higher proportions	609:626	higher proportions	609:626	Our findings showed that cancer samples had higher proportions of Firmicutes (T = 50.5%; N = 28.4%; p = 0.04), specifically of Clostridia (T = 48.3%; N = 19.0%; p = 0.002), and Fusobacteria (T = 0.1%; N = 0.0%; p = 0.02), especially of Fusobacteriia (T = 0.1%; N = 0.0%; p = 0.02), while Bacteroidetes were enriched in healthy samples (T = 35.1%; N = 62.8%; p = 0.06), particularly the class Bacteroidia (T = 35.1%; N = 62.6%; p = 0.06).
29687353	5	28	dep	Firmicutes	631:640	arg1	%					651:651	T = 50.5%	643:651	T = 50.5%; N = 28.4%; p = 0.04	643:672	Our findings showed that cancer samples had higher proportions of Firmicutes (T = 50.5%; N = 28.4%; p = 0.04), specifically of Clostridia (T = 48.3%; N = 19.0%; p = 0.002), and Fusobacteria (T = 0.1%; N = 0.0%; p = 0.02), especially of Fusobacteriia (T = 0.1%; N = 0.0%; p = 0.02), while Bacteroidetes were enriched in healthy samples (T = 35.1%; N = 62.8%; p = 0.06), particularly the class Bacteroidia (T = 35.1%; N = 62.6%; p = 0.06).
29687353	4	29	theme	defined	525:531	arg1	clusters					555:562	defined cancer versus healthy clusters	525:562	defined cancer versus healthy clusters	525:562	Principal coordinate analysis of samples revealed defined cancer versus healthy clusters.
29687353	3	30	theme	healthy	422:428	arg1	individuals					430:440	12 healthy individuals	419:440	12 healthy individuals	419:440	We analyzed the microbiome composition of samples from 11 CRC patients and 12 healthy individuals by 16S rRNA amplicon sequencing.
29687353	7	31	theme	functional	1171:1180	arg1	information					1182:1192	Predicted functional information	1161:1192	Predicted functional information	1161:1192	Predicted functional information showed that bacterial motility proteins, flagellar assembly, and fatty acid biosynthesis metabolism were significantly overrepresented in cancer patients, while amino acid metabolism and glycan biosynthesis were overrepresented in controls.
29687353	9	32	theme	therapeutic	1760:1770	arg1	approaches					1772:1781	population-directed therapeutic approaches	1740:1781	population-directed therapeutic approaches	1740:1781	Results from this study allowed identification of bacterial taxa relevant to the Moroccan population and encourages larger studies to facilitate population-directed therapeutic approaches.
29687353	0	33	theme	colorectal	27:36	arg1	patients					45:52	Moroccan colorectal cancer patients	18:52	Moroccan colorectal cancer patients	18:52	Gut microbiome of Moroccan colorectal cancer patients.
29687353	3	34	theme	microbiome	360:369	arg1	composition					371:381	the microbiome composition	356:381	the microbiome composition of samples from 11 CRC patients and 12 healthy individuals	356:440	We analyzed the microbiome composition of samples from 11 CRC patients and 12 healthy individuals by 16S rRNA amplicon sequencing.
29687353	8	35	theme	functional	1475:1484	arg1	metagenomes					1486:1496	these functional metagenomes	1469:1496	these functional metagenomes	1469:1496	This suggests that involvement of these functional metagenomes is similar and relevant in the carcinogenesis process, independent of the origin of the samples.
29687353	3	36	theme	rRNA	449:452	arg1	sequencing					463:472	rRNA amplicon sequencing	449:472	rRNA amplicon sequencing	449:472	We analyzed the microbiome composition of samples from 11 CRC patients and 12 healthy individuals by 16S rRNA amplicon sequencing.
29687353	0	37	theme	patients	45:52	arg1	microbiome					4:13	Gut microbiome	0:13	Gut microbiome of Moroccan colorectal cancer patients.	0:53	Gut microbiome of Moroccan colorectal cancer patients.
29687353	5	38	theme	higher	609:614	arg1	proportions					616:626	higher proportions	609:626	higher proportions	609:626	Our findings showed that cancer samples had higher proportions of Firmicutes (T = 50.5%; N = 28.4%; p = 0.04), specifically of Clostridia (T = 48.3%; N = 19.0%; p = 0.002), and Fusobacteria (T = 0.1%; N = 0.0%; p = 0.02), especially of Fusobacteriia (T = 0.1%; N = 0.0%; p = 0.02), while Bacteroidetes were enriched in healthy samples (T = 35.1%; N = 62.8%; p = 0.06), particularly the class Bacteroidia (T = 35.1%; N = 62.6%; p = 0.06).
29687353	5	39	dep	Bacteroidia	957:967	arg1	%					978:978	T = 35.1%	970:978	T = 35.1%; N = 62.6%; p = 0.06	970:999	Our findings showed that cancer samples had higher proportions of Firmicutes (T = 50.5%; N = 28.4%; p = 0.04), specifically of Clostridia (T = 48.3%; N = 19.0%; p = 0.002), and Fusobacteria (T = 0.1%; N = 0.0%; p = 0.02), especially of Fusobacteriia (T = 0.1%; N = 0.0%; p = 0.02), while Bacteroidetes were enriched in healthy samples (T = 35.1%; N = 62.8%; p = 0.06), particularly the class Bacteroidia (T = 35.1%; N = 62.6%; p = 0.06).
29687353	3	40	from	individuals	430:440	arg1	composition					371:381	the microbiome composition	356:381	the microbiome composition of samples from 11 CRC patients and 12 healthy individuals	356:440	We analyzed the microbiome composition of samples from 11 CRC patients and 12 healthy individuals by 16S rRNA amplicon sequencing.
29687353	3	40	from	individuals	430:440	arg1	samples					386:392	samples	386:392	samples from 11 CRC patients and 12 healthy individuals	386:440	We analyzed the microbiome composition of samples from 11 CRC patients and 12 healthy individuals by 16S rRNA amplicon sequencing.
29687353	1	41	from	cause	103:107	arg1	Morocco					121:127	Morocco	121:127	Morocco	121:127	Although colorectal cancer is the third leading cause of death in Morocco, there are no studies of the microbiome changes associated with the disease in the Moroccan population.
29687353	5	42	dep	%	763:763	arg1	p = 0.02					776:783	p = 0.02	776:783	p = 0.02	776:783	Our findings showed that cancer samples had higher proportions of Firmicutes (T = 50.5%; N = 28.4%; p = 0.04), specifically of Clostridia (T = 48.3%; N = 19.0%; p = 0.002), and Fusobacteria (T = 0.1%; N = 0.0%; p = 0.02), especially of Fusobacteriia (T = 0.1%; N = 0.0%; p = 0.02), while Bacteroidetes were enriched in healthy samples (T = 35.1%; N = 62.8%; p = 0.06), particularly the class Bacteroidia (T = 35.1%; N = 62.6%; p = 0.06).
29687353	5	42	dep	%	763:763	arg1	%					773:773	N = 0.0%	766:773	T = 0.1%; N = 0.0%; p = 0.02	756:783	Our findings showed that cancer samples had higher proportions of Firmicutes (T = 50.5%; N = 28.4%; p = 0.04), specifically of Clostridia (T = 48.3%; N = 19.0%; p = 0.002), and Fusobacteria (T = 0.1%; N = 0.0%; p = 0.02), especially of Fusobacteriia (T = 0.1%; N = 0.0%; p = 0.02), while Bacteroidetes were enriched in healthy samples (T = 35.1%; N = 62.8%; p = 0.06), particularly the class Bacteroidia (T = 35.1%; N = 62.6%; p = 0.06).
29687353	8	43	theme	origin	1572:1577	arg1	independent					1553:1563	independent	1553:1563	independent	1553:1563	This suggests that involvement of these functional metagenomes is similar and relevant in the carcinogenesis process, independent of the origin of the samples.
29687353	5	44	theme	Firmicutes	631:640	arg1	proportions					616:626	higher proportions	609:626	higher proportions	609:626	Our findings showed that cancer samples had higher proportions of Firmicutes (T = 50.5%; N = 28.4%; p = 0.04), specifically of Clostridia (T = 48.3%; N = 19.0%; p = 0.002), and Fusobacteria (T = 0.1%; N = 0.0%; p = 0.02), especially of Fusobacteriia (T = 0.1%; N = 0.0%; p = 0.02), while Bacteroidetes were enriched in healthy samples (T = 35.1%; N = 62.8%; p = 0.06), particularly the class Bacteroidia (T = 35.1%; N = 62.6%; p = 0.06).
29687353	3	45	from	patients	406:413	arg1	composition					371:381	the microbiome composition	356:381	the microbiome composition of samples from 11 CRC patients and 12 healthy individuals	356:440	We analyzed the microbiome composition of samples from 11 CRC patients and 12 healthy individuals by 16S rRNA amplicon sequencing.
29687353	3	45	from	patients	406:413	arg1	samples					386:392	samples	386:392	samples from 11 CRC patients and 12 healthy individuals	386:440	We analyzed the microbiome composition of samples from 11 CRC patients and 12 healthy individuals by 16S rRNA amplicon sequencing.
29687353	7	46	theme	acid	1361:1364	arg1	metabolism					1366:1375	amino acid metabolism	1355:1375	amino acid metabolism	1355:1375	Predicted functional information showed that bacterial motility proteins, flagellar assembly, and fatty acid biosynthesis metabolism were significantly overrepresented in cancer patients, while amino acid metabolism and glycan biosynthesis were overrepresented in controls.
29687353	8	47	theme	carcinogenesis	1529:1542	arg1	process					1544:1550	the carcinogenesis process	1525:1550	the carcinogenesis process	1525:1550	This suggests that involvement of these functional metagenomes is similar and relevant in the carcinogenesis process, independent of the origin of the samples.
29687353	5	48	dep	Fusobacteria	742:753	arg1	%					763:763	T = 0.1%	756:763	T = 0.1%; N = 0.0%; p = 0.02	756:783	Our findings showed that cancer samples had higher proportions of Firmicutes (T = 50.5%; N = 28.4%; p = 0.04), specifically of Clostridia (T = 48.3%; N = 19.0%; p = 0.002), and Fusobacteria (T = 0.1%; N = 0.0%; p = 0.02), especially of Fusobacteriia (T = 0.1%; N = 0.0%; p = 0.02), while Bacteroidetes were enriched in healthy samples (T = 35.1%; N = 62.8%; p = 0.06), particularly the class Bacteroidia (T = 35.1%; N = 62.6%; p = 0.06).
29687353	7	49	theme	cancer	1332:1337	arg1	patients					1339:1346	cancer patients	1332:1346	cancer patients	1332:1346	Predicted functional information showed that bacterial motility proteins, flagellar assembly, and fatty acid biosynthesis metabolism were significantly overrepresented in cancer patients, while amino acid metabolism and glycan biosynthesis were overrepresented in controls.
29687353	5	50	contain	had	605:607	arg2	proportions					616:626	higher proportions	609:626	higher proportions	609:626	Our findings showed that cancer samples had higher proportions of Firmicutes (T = 50.5%; N = 28.4%; p = 0.04), specifically of Clostridia (T = 48.3%; N = 19.0%; p = 0.002), and Fusobacteria (T = 0.1%; N = 0.0%; p = 0.02), especially of Fusobacteriia (T = 0.1%; N = 0.0%; p = 0.02), while Bacteroidetes were enriched in healthy samples (T = 35.1%; N = 62.8%; p = 0.06), particularly the class Bacteroidia (T = 35.1%; N = 62.6%; p = 0.06).
29687353	5	50	contain	had	605:607	arg1	samples					597:603	cancer samples	590:603	cancer samples	590:603	Our findings showed that cancer samples had higher proportions of Firmicutes (T = 50.5%; N = 28.4%; p = 0.04), specifically of Clostridia (T = 48.3%; N = 19.0%; p = 0.002), and Fusobacteria (T = 0.1%; N = 0.0%; p = 0.02), especially of Fusobacteriia (T = 0.1%; N = 0.0%; p = 0.02), while Bacteroidetes were enriched in healthy samples (T = 35.1%; N = 62.8%; p = 0.06), particularly the class Bacteroidia (T = 35.1%; N = 62.6%; p = 0.06).
29687353	2	51	theme	cancer	302:307	arg1	patients					309:316	Moroccan cancer patients	293:316	Moroccan cancer patients	293:316	The aim of our study was to compare the stool microbiome of Moroccan cancer patients with healthy individuals.
29687353	8	52	from	similar	1501:1507	arg1	process					1544:1550	the carcinogenesis process	1525:1550	the carcinogenesis process	1525:1550	This suggests that involvement of these functional metagenomes is similar and relevant in the carcinogenesis process, independent of the origin of the samples.
29687353	2	53	theme	stool	273:277	arg1	microbiome					279:288	the stool microbiome	269:288	the stool microbiome of Moroccan cancer patients with healthy individuals	269:341	The aim of our study was to compare the stool microbiome of Moroccan cancer patients with healthy individuals.
29687353	1	54	theme	changes	169:175	arg1	studies					143:149	no studies	140:149	no studies of the microbiome changes associated with the disease in the Moroccan population	140:230	Although colorectal cancer is the third leading cause of death in Morocco, there are no studies of the microbiome changes associated with the disease in the Moroccan population.
29687353	7	55	theme	biosynthesis	1270:1281	arg1	metabolism					1283:1292	fatty acid biosynthesis metabolism	1259:1292	fatty acid biosynthesis metabolism	1259:1292	Predicted functional information showed that bacterial motility proteins, flagellar assembly, and fatty acid biosynthesis metabolism were significantly overrepresented in cancer patients, while amino acid metabolism and glycan biosynthesis were overrepresented in controls.
29687353	5	56	dep	samples	892:898	arg1	%					909:909	T = 35.1%	901:909	T = 35.1%; N = 62.8%; p = 0.06	901:930	Our findings showed that cancer samples had higher proportions of Firmicutes (T = 50.5%; N = 28.4%; p = 0.04), specifically of Clostridia (T = 48.3%; N = 19.0%; p = 0.002), and Fusobacteria (T = 0.1%; N = 0.0%; p = 0.02), especially of Fusobacteriia (T = 0.1%; N = 0.0%; p = 0.02), while Bacteroidetes were enriched in healthy samples (T = 35.1%; N = 62.8%; p = 0.06), particularly the class Bacteroidia (T = 35.1%; N = 62.6%; p = 0.06).
29687353	2	57	theme	Moroccan	293:300	arg1	patients					309:316	Moroccan cancer patients	293:316	Moroccan cancer patients	293:316	The aim of our study was to compare the stool microbiome of Moroccan cancer patients with healthy individuals.
29687353	7	58	theme	fatty	1259:1263	arg1	metabolism					1283:1292	fatty acid biosynthesis metabolism	1259:1292	fatty acid biosynthesis metabolism	1259:1292	Predicted functional information showed that bacterial motility proteins, flagellar assembly, and fatty acid biosynthesis metabolism were significantly overrepresented in cancer patients, while amino acid metabolism and glycan biosynthesis were overrepresented in controls.
29687353	4	59	theme	samples	508:514	arg1	analysis					496:503	Principal coordinate analysis	475:503	Principal coordinate analysis of samples	475:514	Principal coordinate analysis of samples revealed defined cancer versus healthy clusters.
29687353	8	60	from	process	1544:1550	arg1	similar					1501:1507	similar	1501:1507	similar	1501:1507	This suggests that involvement of these functional metagenomes is similar and relevant in the carcinogenesis process, independent of the origin of the samples.
29687353	9	61	theme	taxa	1655:1658	arg1	identification					1627:1640	identification	1627:1640	identification of bacterial taxa relevant to the Moroccan population	1627:1694	Results from this study allowed identification of bacterial taxa relevant to the Moroccan population and encourages larger studies to facilitate population-directed therapeutic approaches.
29687353	7	62	theme	flagellar	1235:1243	arg1	assembly					1245:1252	flagellar assembly	1235:1252	flagellar assembly	1235:1252	Predicted functional information showed that bacterial motility proteins, flagellar assembly, and fatty acid biosynthesis metabolism were significantly overrepresented in cancer patients, while amino acid metabolism and glycan biosynthesis were overrepresented in controls.
29687353	3	63	from	composition	371:381	arg1	patients					406:413	11 CRC patients	399:413	11 CRC patients	399:413	We analyzed the microbiome composition of samples from 11 CRC patients and 12 healthy individuals by 16S rRNA amplicon sequencing.
29687353	3	63	from	composition	371:381	arg1	individuals					430:440	12 healthy individuals	419:440	12 healthy individuals	419:440	We analyzed the microbiome composition of samples from 11 CRC patients and 12 healthy individuals by 16S rRNA amplicon sequencing.
29687353	5	64	theme	Fusobacteriia	801:813	arg1	proportions					616:626	higher proportions	609:626	higher proportions	609:626	Our findings showed that cancer samples had higher proportions of Firmicutes (T = 50.5%; N = 28.4%; p = 0.04), specifically of Clostridia (T = 48.3%; N = 19.0%; p = 0.002), and Fusobacteria (T = 0.1%; N = 0.0%; p = 0.02), especially of Fusobacteriia (T = 0.1%; N = 0.0%; p = 0.02), while Bacteroidetes were enriched in healthy samples (T = 35.1%; N = 62.8%; p = 0.06), particularly the class Bacteroidia (T = 35.1%; N = 62.6%; p = 0.06).
29687353	9	65	from	study	1613:1617	arg1	Results					1595:1601	Results	1595:1601	Results from this study	1595:1617	Results from this study allowed identification of bacterial taxa relevant to the Moroccan population and encourages larger studies to facilitate population-directed therapeutic approaches.
29687353	9	66	theme	Moroccan	1676:1683	arg1	population					1685:1694	the Moroccan population	1672:1694	the Moroccan population	1672:1694	Results from this study allowed identification of bacterial taxa relevant to the Moroccan population and encourages larger studies to facilitate population-directed therapeutic approaches.
29687353	4	67	theme	coordinate	485:494	arg1	analysis					496:503	Principal coordinate analysis	475:503	Principal coordinate analysis of samples	475:514	Principal coordinate analysis of samples revealed defined cancer versus healthy clusters.
29687353	9	68	theme	larger	1711:1716	arg1	studies					1718:1724	larger studies	1711:1724	larger studies	1711:1724	Results from this study allowed identification of bacterial taxa relevant to the Moroccan population and encourages larger studies to facilitate population-directed therapeutic approaches.
29687353	5	69	dep	%	823:823	arg1	p = 0.02					836:843	p = 0.02	836:843	p = 0.02	836:843	Our findings showed that cancer samples had higher proportions of Firmicutes (T = 50.5%; N = 28.4%; p = 0.04), specifically of Clostridia (T = 48.3%; N = 19.0%; p = 0.002), and Fusobacteria (T = 0.1%; N = 0.0%; p = 0.02), especially of Fusobacteriia (T = 0.1%; N = 0.0%; p = 0.02), while Bacteroidetes were enriched in healthy samples (T = 35.1%; N = 62.8%; p = 0.06), particularly the class Bacteroidia (T = 35.1%; N = 62.6%; p = 0.06).
29687353	5	69	dep	%	823:823	arg1	%					833:833	N = 0.0%	826:833	T = 0.1%; N = 0.0%; p = 0.02	816:843	Our findings showed that cancer samples had higher proportions of Firmicutes (T = 50.5%; N = 28.4%; p = 0.04), specifically of Clostridia (T = 48.3%; N = 19.0%; p = 0.002), and Fusobacteria (T = 0.1%; N = 0.0%; p = 0.02), especially of Fusobacteriia (T = 0.1%; N = 0.0%; p = 0.02), while Bacteroidetes were enriched in healthy samples (T = 35.1%; N = 62.8%; p = 0.06), particularly the class Bacteroidia (T = 35.1%; N = 62.6%; p = 0.06).
29687353	1	70	from	Morocco	121:127	arg1	cancer					75:80	colorectal cancer	64:80	colorectal cancer	64:80	Although colorectal cancer is the third leading cause of death in Morocco, there are no studies of the microbiome changes associated with the disease in the Moroccan population.
29687353	1	70	from	Morocco	121:127	arg1	cause					103:107	the third leading cause	85:107	the third leading cause of death in Morocco	85:127	Although colorectal cancer is the third leading cause of death in Morocco, there are no studies of the microbiome changes associated with the disease in the Moroccan population.
29687353	5	71	dep	%	651:651	arg1	%					662:662	N = 28.4%	654:662	T = 50.5%; N = 28.4%; p = 0.04	643:672	Our findings showed that cancer samples had higher proportions of Firmicutes (T = 50.5%; N = 28.4%; p = 0.04), specifically of Clostridia (T = 48.3%; N = 19.0%; p = 0.002), and Fusobacteria (T = 0.1%; N = 0.0%; p = 0.02), especially of Fusobacteriia (T = 0.1%; N = 0.0%; p = 0.02), while Bacteroidetes were enriched in healthy samples (T = 35.1%; N = 62.8%; p = 0.06), particularly the class Bacteroidia (T = 35.1%; N = 62.6%; p = 0.06).
29687353	5	71	dep	%	651:651	arg1	p = 0.04					665:672	p = 0.04	665:672	p = 0.04	665:672	Our findings showed that cancer samples had higher proportions of Firmicutes (T = 50.5%; N = 28.4%; p = 0.04), specifically of Clostridia (T = 48.3%; N = 19.0%; p = 0.002), and Fusobacteria (T = 0.1%; N = 0.0%; p = 0.02), especially of Fusobacteriia (T = 0.1%; N = 0.0%; p = 0.02), while Bacteroidetes were enriched in healthy samples (T = 35.1%; N = 62.8%; p = 0.06), particularly the class Bacteroidia (T = 35.1%; N = 62.6%; p = 0.06).
29687353	4	72	theme	cancer	533:538	arg1	clusters					555:562	defined cancer versus healthy clusters	525:562	defined cancer versus healthy clusters	525:562	Principal coordinate analysis of samples revealed defined cancer versus healthy clusters.
29687353	0	73	theme	Moroccan	18:25	arg1	patients					45:52	Moroccan colorectal cancer patients	18:52	Moroccan colorectal cancer patients	18:52	Gut microbiome of Moroccan colorectal cancer patients.
29687353	1	74	from	death	112:116	arg1	Morocco					121:127	Morocco	121:127	Morocco	121:127	Although colorectal cancer is the third leading cause of death in Morocco, there are no studies of the microbiome changes associated with the disease in the Moroccan population.
29687353	1	75	theme	leading	95:101	arg1	cancer					75:80	colorectal cancer	64:80	colorectal cancer	64:80	Although colorectal cancer is the third leading cause of death in Morocco, there are no studies of the microbiome changes associated with the disease in the Moroccan population.
29687353	1	75	theme	leading	95:101	arg1	cause					103:107	the third leading cause	85:107	the third leading cause of death in Morocco	85:127	Although colorectal cancer is the third leading cause of death in Morocco, there are no studies of the microbiome changes associated with the disease in the Moroccan population.
29687353	7	76	theme	Predicted	1161:1169	arg1	information					1182:1192	Predicted functional information	1161:1192	Predicted functional information	1161:1192	Predicted functional information showed that bacterial motility proteins, flagellar assembly, and fatty acid biosynthesis metabolism were significantly overrepresented in cancer patients, while amino acid metabolism and glycan biosynthesis were overrepresented in controls.
29687353	9	77	theme	population-directed	1740:1758	arg1	approaches					1772:1781	population-directed therapeutic approaches	1740:1781	population-directed therapeutic approaches	1740:1781	Results from this study allowed identification of bacterial taxa relevant to the Moroccan population and encourages larger studies to facilitate population-directed therapeutic approaches.
29687353	4	78	theme	healthy	547:553	arg1	clusters					555:562	defined cancer versus healthy clusters	525:562	defined cancer versus healthy clusters	525:562	Principal coordinate analysis of samples revealed defined cancer versus healthy clusters.
29687353	0	79	theme	cancer	38:43	arg1	patients					45:52	Moroccan colorectal cancer patients	18:52	Moroccan colorectal cancer patients	18:52	Gut microbiome of Moroccan colorectal cancer patients.
29687353	2	80	with	microbiome	279:288	arg1	individuals					331:341	healthy individuals	323:341	healthy individuals	323:341	The aim of our study was to compare the stool microbiome of Moroccan cancer patients with healthy individuals.
29687353	3	81	theme	samples	386:392	arg1	composition					371:381	the microbiome composition	356:381	the microbiome composition of samples from 11 CRC patients and 12 healthy individuals	356:440	We analyzed the microbiome composition of samples from 11 CRC patients and 12 healthy individuals by 16S rRNA amplicon sequencing.
29687353	8	82	theme	metagenomes	1486:1496	arg1	involvement					1454:1464	involvement	1454:1464	involvement of these functional metagenomes	1454:1496	This suggests that involvement of these functional metagenomes is similar and relevant in the carcinogenesis process, independent of the origin of the samples.
29687353	3	83	theme	amplicon	454:461	arg1	sequencing					463:472	rRNA amplicon sequencing	449:472	rRNA amplicon sequencing	449:472	We analyzed the microbiome composition of samples from 11 CRC patients and 12 healthy individuals by 16S rRNA amplicon sequencing.
29687353	5	84	theme	healthy	884:890	arg1	samples					892:898	healthy samples	884:898	healthy samples (T = 35.1%; N = 62.8%; p = 0.06)	884:931	Our findings showed that cancer samples had higher proportions of Firmicutes (T = 50.5%; N = 28.4%; p = 0.04), specifically of Clostridia (T = 48.3%; N = 19.0%; p = 0.002), and Fusobacteria (T = 0.1%; N = 0.0%; p = 0.02), especially of Fusobacteriia (T = 0.1%; N = 0.0%; p = 0.02), while Bacteroidetes were enriched in healthy samples (T = 35.1%; N = 62.8%; p = 0.06), particularly the class Bacteroidia (T = 35.1%; N = 62.6%; p = 0.06).
29687353	5	84	theme	healthy	884:890	arg1	Bacteroidia					957:967	the class Bacteroidia	947:967	the class Bacteroidia (T = 35.1%; N = 62.6%; p = 0.06)	947:1000	Our findings showed that cancer samples had higher proportions of Firmicutes (T = 50.5%; N = 28.4%; p = 0.04), specifically of Clostridia (T = 48.3%; N = 19.0%; p = 0.002), and Fusobacteria (T = 0.1%; N = 0.0%; p = 0.02), especially of Fusobacteriia (T = 0.1%; N = 0.0%; p = 0.02), while Bacteroidetes were enriched in healthy samples (T = 35.1%; N = 62.8%; p = 0.06), particularly the class Bacteroidia (T = 35.1%; N = 62.6%; p = 0.06).
29687353	2	85	theme	healthy	323:329	arg1	individuals					331:341	healthy individuals	323:341	healthy individuals	323:341	The aim of our study was to compare the stool microbiome of Moroccan cancer patients with healthy individuals.
29687353	8	86	theme	samples	1586:1592	arg1	origin					1572:1577	the origin	1568:1577	the origin of the samples	1568:1592	This suggests that involvement of these functional metagenomes is similar and relevant in the carcinogenesis process, independent of the origin of the samples.
29687353	1	87	theme	Moroccan	212:219	arg1	population					221:230	the Moroccan population	208:230	the Moroccan population	208:230	Although colorectal cancer is the third leading cause of death in Morocco, there are no studies of the microbiome changes associated with the disease in the Moroccan population.
29538769	1	0	dep	potato	255:260	arg1	tuberosum					271:279	Solanum tuberosum	263:279	Solanum tuberosum	263:279	The importance of a plastidial soluble inorganic pyrophosphatase (psPPase) and an ATP/ADP translocator (NTT) for starch composition and tuber formation in potato (Solanum tuberosum) was evaluated by individual and simultaneous down-regulation of the corresponding endogenous genes.
29538769	1	1	theme	plastidial	120:129	arg1	psPPase					166:172	psPPase	166:172	psPPase	166:172	The importance of a plastidial soluble inorganic pyrophosphatase (psPPase) and an ATP/ADP translocator (NTT) for starch composition and tuber formation in potato (Solanum tuberosum) was evaluated by individual and simultaneous down-regulation of the corresponding endogenous genes.
29538769	1	1	theme	plastidial	120:129	arg1	pyrophosphatase					149:163	a plastidial soluble inorganic pyrophosphatase	118:163	a plastidial soluble inorganic pyrophosphatase (psPPase)	118:173	The importance of a plastidial soluble inorganic pyrophosphatase (psPPase) and an ATP/ADP translocator (NTT) for starch composition and tuber formation in potato (Solanum tuberosum) was evaluated by individual and simultaneous down-regulation of the corresponding endogenous genes.
29538769	3	2	theme	higher	679:684	arg1	yield					699:703	higher fresh weight yield	679:703	higher fresh weight yield	679:703	Single-gene down-regulation of either StpsPPase or StNTT resulted in increased tuber numbers per plant and higher fresh weight yield.
29538769	1	3	theme	soluble	131:137	arg1	psPPase					166:172	psPPase	166:172	psPPase	166:172	The importance of a plastidial soluble inorganic pyrophosphatase (psPPase) and an ATP/ADP translocator (NTT) for starch composition and tuber formation in potato (Solanum tuberosum) was evaluated by individual and simultaneous down-regulation of the corresponding endogenous genes.
29538769	1	3	theme	soluble	131:137	arg1	pyrophosphatase					149:163	a plastidial soluble inorganic pyrophosphatase	118:163	a plastidial soluble inorganic pyrophosphatase (psPPase)	118:173	The importance of a plastidial soluble inorganic pyrophosphatase (psPPase) and an ATP/ADP translocator (NTT) for starch composition and tuber formation in potato (Solanum tuberosum) was evaluated by individual and simultaneous down-regulation of the corresponding endogenous genes.
29538769	1	4	theme	starch	213:218	arg1	composition					220:230	starch composition	213:230	starch composition	213:230	The importance of a plastidial soluble inorganic pyrophosphatase (psPPase) and an ATP/ADP translocator (NTT) for starch composition and tuber formation in potato (Solanum tuberosum) was evaluated by individual and simultaneous down-regulation of the corresponding endogenous genes.
29538769	0	5	theme	tuber	73:77	arg1	formation					79:87	tuber formation	73:87	tuber formation	73:87	Inhibition of plastid PPase and NTT leads to major changes in starch and tuber formation in potato.
29538769	1	6	theme	individual	299:308	arg1	down-regulation					327:341	individual and simultaneous down-regulation	299:341	down-regulation	327:341	The importance of a plastidial soluble inorganic pyrophosphatase (psPPase) and an ATP/ADP translocator (NTT) for starch composition and tuber formation in potato (Solanum tuberosum) was evaluated by individual and simultaneous down-regulation of the corresponding endogenous genes.
29538769	8	7	theme	PPi	1352:1354	arg1	hydrolysis					1356:1365	functional PPi hydrolysis	1341:1365	functional PPi hydrolysis in combination with efficient ATP import	1341:1406	Additionally, it demonstrates that functional PPi hydrolysis in combination with efficient ATP import is essential for tuber formation and development.
29538769	6	8	theme	Increased	974:982	arg1	amounts					984:990	Increased amounts	974:990	Increased amounts of intermediates of vitamin C biosynthesis	974:1033	Increased amounts of intermediates of vitamin C biosynthesis were also observed.
29538769	6	8	theme	Increased	974:982	arg1	intermediates					995:1007	intermediates	995:1007	intermediates of vitamin C biosynthesis	995:1033	Increased amounts of intermediates of vitamin C biosynthesis were also observed.
29538769	7	9	theme	potato	1184:1189	arg1	tubers					1191:1196	potato tubers	1184:1196	potato tubers	1184:1196	This study suggests that hydrolysis of pyrophosphate (PPi) by action of a psPPase is vital for functional starch accumulation in potato tubers and that no additional mechanism for consuming, hydrolysing, or exporting PPi exists in the studied tissue.
29538769	7	10	from	accumulation	1168:1179	arg1	tubers					1191:1196	potato tubers	1184:1196	potato tubers	1184:1196	This study suggests that hydrolysis of pyrophosphate (PPi) by action of a psPPase is vital for functional starch accumulation in potato tubers and that no additional mechanism for consuming, hydrolysing, or exporting PPi exists in the studied tissue.
29538769	5	11	from	amounts	868:874	arg1	content					942:948	ADP-glucose content	930:948	ADP-glucose content of the StpsPPase lines	930:971	Analysis of metabolites revealed altered amounts of sugar intermediates, and a substantial increase in ADP-glucose content of the StpsPPase lines.
29538769	5	12	theme	sugar	879:883	arg1	intermediates					885:897	sugar intermediates	879:897	sugar intermediates	879:897	Analysis of metabolites revealed altered amounts of sugar intermediates, and a substantial increase in ADP-glucose content of the StpsPPase lines.
29538769	5	13	theme	substantial	906:916	arg1	increase					918:925	a substantial increase	904:925	a substantial increase in ADP-glucose content of the StpsPPase lines	904:971	Analysis of metabolites revealed altered amounts of sugar intermediates, and a substantial increase in ADP-glucose content of the StpsPPase lines.
29538769	7	14	theme	starch	1161:1166	arg1	accumulation					1168:1179	functional starch accumulation	1150:1179	functional starch accumulation in potato tubers	1150:1196	This study suggests that hydrolysis of pyrophosphate (PPi) by action of a psPPase is vital for functional starch accumulation in potato tubers and that no additional mechanism for consuming, hydrolysing, or exporting PPi exists in the studied tissue.
29538769	1	15	theme	simultaneous	314:325	arg1	down-regulation					327:341	individual and simultaneous down-regulation	299:341	down-regulation	327:341	The importance of a plastidial soluble inorganic pyrophosphatase (psPPase) and an ATP/ADP translocator (NTT) for starch composition and tuber formation in potato (Solanum tuberosum) was evaluated by individual and simultaneous down-regulation of the corresponding endogenous genes.
29538769	2	16	dep	smaller	489:495	arg1	size					470:473	granule size	462:473	granule size	462:473	Starch and amylose content of the transgenic lines were considerably lower, and granule size substantially smaller, with down-regulation of StpsPPase generating the most pronounced effects.
29538769	5	17	theme	StpsPPase	957:965	arg1	lines					967:971	the StpsPPase lines	953:971	the StpsPPase lines	953:971	Analysis of metabolites revealed altered amounts of sugar intermediates, and a substantial increase in ADP-glucose content of the StpsPPase lines.
29538769	1	18	theme	inorganic	139:147	arg1	psPPase					166:172	psPPase	166:172	psPPase	166:172	The importance of a plastidial soluble inorganic pyrophosphatase (psPPase) and an ATP/ADP translocator (NTT) for starch composition and tuber formation in potato (Solanum tuberosum) was evaluated by individual and simultaneous down-regulation of the corresponding endogenous genes.
29538769	1	18	theme	inorganic	139:147	arg1	pyrophosphatase					149:163	a plastidial soluble inorganic pyrophosphatase	118:163	a plastidial soluble inorganic pyrophosphatase (psPPase)	118:173	The importance of a plastidial soluble inorganic pyrophosphatase (psPPase) and an ATP/ADP translocator (NTT) for starch composition and tuber formation in potato (Solanum tuberosum) was evaluated by individual and simultaneous down-regulation of the corresponding endogenous genes.
29538769	4	19	theme	distorted	809:817	arg1	tubers					819:824	only a few, small and distorted tubers	787:824	only a few, small and distorted tubers	787:824	In contrast, when both genes were inhibited simultaneously, some lines developed only a few, small and distorted tubers.
29538769	5	20	theme	lines	967:971	arg1	content					942:948	ADP-glucose content	930:948	ADP-glucose content of the StpsPPase lines	930:971	Analysis of metabolites revealed altered amounts of sugar intermediates, and a substantial increase in ADP-glucose content of the StpsPPase lines.
29538769	1	21	theme	pyrophosphatase	149:163	arg1	importance					104:113	The importance	100:113	The importance of a plastidial soluble inorganic pyrophosphatase (psPPase) and an ATP/ADP translocator (NTT) for starch composition and tuber formation in potato (Solanum tuberosum)	100:280	The importance of a plastidial soluble inorganic pyrophosphatase (psPPase) and an ATP/ADP translocator (NTT) for starch composition and tuber formation in potato (Solanum tuberosum) was evaluated by individual and simultaneous down-regulation of the corresponding endogenous genes.
29538769	2	22	theme	transgenic	416:425	arg1	lines					427:431	the transgenic lines	412:431	the transgenic lines	412:431	Starch and amylose content of the transgenic lines were considerably lower, and granule size substantially smaller, with down-regulation of StpsPPase generating the most pronounced effects.
29538769	8	23	theme	ATP	1397:1399	arg1	import					1401:1406	efficient ATP import	1387:1406	efficient ATP import	1387:1406	Additionally, it demonstrates that functional PPi hydrolysis in combination with efficient ATP import is essential for tuber formation and development.
29538769	7	24	theme	exporting	1262:1270	arg1	PPi					1272:1274	exporting PPi	1262:1274	exporting PPi	1262:1274	This study suggests that hydrolysis of pyrophosphate (PPi) by action of a psPPase is vital for functional starch accumulation in potato tubers and that no additional mechanism for consuming, hydrolysing, or exporting PPi exists in the studied tissue.
29538769	8	25	theme	functional	1341:1350	arg1	hydrolysis					1356:1365	functional PPi hydrolysis	1341:1365	functional PPi hydrolysis in combination with efficient ATP import	1341:1406	Additionally, it demonstrates that functional PPi hydrolysis in combination with efficient ATP import is essential for tuber formation and development.
29538769	3	26	theme	fresh	686:690	arg1	yield					699:703	higher fresh weight yield	679:703	higher fresh weight yield	679:703	Single-gene down-regulation of either StpsPPase or StNTT resulted in increased tuber numbers per plant and higher fresh weight yield.
29538769	7	27	theme	functional	1150:1159	arg1	accumulation					1168:1179	functional starch accumulation	1150:1179	functional starch accumulation in potato tubers	1150:1196	This study suggests that hydrolysis of pyrophosphate (PPi) by action of a psPPase is vital for functional starch accumulation in potato tubers and that no additional mechanism for consuming, hydrolysing, or exporting PPi exists in the studied tissue.
29538769	1	28	theme	tuber	236:240	arg1	formation					242:250	tuber formation	236:250	tuber formation	236:250	The importance of a plastidial soluble inorganic pyrophosphatase (psPPase) and an ATP/ADP translocator (NTT) for starch composition and tuber formation in potato (Solanum tuberosum) was evaluated by individual and simultaneous down-regulation of the corresponding endogenous genes.
29538769	1	29	from	formation	242:250	arg1	potato					255:260	potato	255:260	potato (Solanum tuberosum)	255:280	The importance of a plastidial soluble inorganic pyrophosphatase (psPPase) and an ATP/ADP translocator (NTT) for starch composition and tuber formation in potato (Solanum tuberosum) was evaluated by individual and simultaneous down-regulation of the corresponding endogenous genes.
29538769	3	30	theme	tuber	651:655	arg1	numbers					657:663	increased tuber numbers	641:663	increased tuber numbers per plant and higher fresh weight yield	641:703	Single-gene down-regulation of either StpsPPase or StNTT resulted in increased tuber numbers per plant and higher fresh weight yield.
29538769	0	31	theme	PPase	22:26	arg1	Inhibition					0:9	Inhibition	0:9	Inhibition of plastid PPase and NTT	0:34	Inhibition of plastid PPase and NTT leads to major changes in starch and tuber formation in potato.
29538769	1	32	from	composition	220:230	arg1	potato					255:260	potato	255:260	potato (Solanum tuberosum)	255:280	The importance of a plastidial soluble inorganic pyrophosphatase (psPPase) and an ATP/ADP translocator (NTT) for starch composition and tuber formation in potato (Solanum tuberosum) was evaluated by individual and simultaneous down-regulation of the corresponding endogenous genes.
29538769	4	33	theme	small	799:803	arg1	tubers					819:824	only a few, small and distorted tubers	787:824	only a few, small and distorted tubers	787:824	In contrast, when both genes were inhibited simultaneously, some lines developed only a few, small and distorted tubers.
29538769	5	34	theme	ADP-glucose	930:940	arg1	content					942:948	ADP-glucose content	930:948	ADP-glucose content of the StpsPPase lines	930:971	Analysis of metabolites revealed altered amounts of sugar intermediates, and a substantial increase in ADP-glucose content of the StpsPPase lines.
29538769	7	35	theme	pyrophosphate	1094:1106	arg1	hydrolysis					1080:1089	hydrolysis	1080:1089	hydrolysis of pyrophosphate (PPi) by action of a psPPase	1080:1135	This study suggests that hydrolysis of pyrophosphate (PPi) by action of a psPPase is vital for functional starch accumulation in potato tubers and that no additional mechanism for consuming, hydrolysing, or exporting PPi exists in the studied tissue.
29538769	2	36	theme	amylose	393:399	arg1	content					401:407	Starch and amylose content	382:407	Starch and amylose content of the transgenic lines	382:431	Starch and amylose content of the transgenic lines were considerably lower, and granule size substantially smaller, with down-regulation of StpsPPase generating the most pronounced effects.
29538769	0	37	theme	plastid	14:20	arg1	PPase					22:26	plastid PPase	14:26	plastid PPase	14:26	Inhibition of plastid PPase and NTT leads to major changes in starch and tuber formation in potato.
29538769	8	38	with	combination	1370:1380	arg1	import					1401:1406	efficient ATP import	1387:1406	efficient ATP import	1387:1406	Additionally, it demonstrates that functional PPi hydrolysis in combination with efficient ATP import is essential for tuber formation and development.
29538769	2	39	theme	StpsPPase	522:530	arg1	down-regulation					503:517	down-regulation	503:517	down-regulation of StpsPPase generating the most pronounced effects	503:569	Starch and amylose content of the transgenic lines were considerably lower, and granule size substantially smaller, with down-regulation of StpsPPase generating the most pronounced effects.
29538769	3	40	theme	increased	641:649	arg1	numbers					657:663	increased tuber numbers	641:663	increased tuber numbers per plant and higher fresh weight yield	641:703	Single-gene down-regulation of either StpsPPase or StNTT resulted in increased tuber numbers per plant and higher fresh weight yield.
29538769	5	41	theme	intermediates	885:897	arg1	amounts					868:874	altered amounts	860:874	altered amounts of sugar intermediates	860:897	Analysis of metabolites revealed altered amounts of sugar intermediates, and a substantial increase in ADP-glucose content of the StpsPPase lines.
29538769	5	41	theme	intermediates	885:897	arg1	intermediates					885:897	sugar intermediates	879:897	sugar intermediates	879:897	Analysis of metabolites revealed altered amounts of sugar intermediates, and a substantial increase in ADP-glucose content of the StpsPPase lines.
29538769	5	41	theme	intermediates	885:897	arg1	increase					918:925	a substantial increase	904:925	a substantial increase in ADP-glucose content of the StpsPPase lines	904:971	Analysis of metabolites revealed altered amounts of sugar intermediates, and a substantial increase in ADP-glucose content of the StpsPPase lines.
29538769	0	42	theme	NTT	32:34	arg1	Inhibition					0:9	Inhibition	0:9	Inhibition of plastid PPase and NTT	0:34	Inhibition of plastid PPase and NTT leads to major changes in starch and tuber formation in potato.
29538769	7	43	theme	studied	1290:1296	arg1	tissue					1298:1303	the studied tissue	1286:1303	the studied tissue	1286:1303	This study suggests that hydrolysis of pyrophosphate (PPi) by action of a psPPase is vital for functional starch accumulation in potato tubers and that no additional mechanism for consuming, hydrolysing, or exporting PPi exists in the studied tissue.
29538769	1	44	theme	corresponding	350:362	arg1	genes					375:379	the corresponding endogenous genes	346:379	the corresponding endogenous genes	346:379	The importance of a plastidial soluble inorganic pyrophosphatase (psPPase) and an ATP/ADP translocator (NTT) for starch composition and tuber formation in potato (Solanum tuberosum) was evaluated by individual and simultaneous down-regulation of the corresponding endogenous genes.
29538769	5	45	theme	altered	860:866	arg1	amounts					868:874	altered amounts	860:874	altered amounts of sugar intermediates	860:897	Analysis of metabolites revealed altered amounts of sugar intermediates, and a substantial increase in ADP-glucose content of the StpsPPase lines.
29538769	5	45	theme	altered	860:866	arg1	intermediates					885:897	sugar intermediates	879:897	sugar intermediates	879:897	Analysis of metabolites revealed altered amounts of sugar intermediates, and a substantial increase in ADP-glucose content of the StpsPPase lines.
29538769	2	46	theme	Starch	382:387	arg1	content					401:407	Starch and amylose content	382:407	Starch and amylose content of the transgenic lines	382:431	Starch and amylose content of the transgenic lines were considerably lower, and granule size substantially smaller, with down-regulation of StpsPPase generating the most pronounced effects.
29538769	7	47	theme	psPPase	1129:1135	arg1	action					1117:1122	action	1117:1122	action of a psPPase	1117:1135	This study suggests that hydrolysis of pyrophosphate (PPi) by action of a psPPase is vital for functional starch accumulation in potato tubers and that no additional mechanism for consuming, hydrolysing, or exporting PPi exists in the studied tissue.
29538769	1	48	theme	endogenous	364:373	arg1	genes					375:379	the corresponding endogenous genes	346:379	the corresponding endogenous genes	346:379	The importance of a plastidial soluble inorganic pyrophosphatase (psPPase) and an ATP/ADP translocator (NTT) for starch composition and tuber formation in potato (Solanum tuberosum) was evaluated by individual and simultaneous down-regulation of the corresponding endogenous genes.
29538769	4	49	theme	few	794:796	arg1	tubers					819:824	only a few, small and distorted tubers	787:824	only a few, small and distorted tubers	787:824	In contrast, when both genes were inhibited simultaneously, some lines developed only a few, small and distorted tubers.
29538769	1	50	theme	ATP/ADP	182:188	arg1	NTT					204:206	NTT	204:206	NTT	204:206	The importance of a plastidial soluble inorganic pyrophosphatase (psPPase) and an ATP/ADP translocator (NTT) for starch composition and tuber formation in potato (Solanum tuberosum) was evaluated by individual and simultaneous down-regulation of the corresponding endogenous genes.
29538769	1	50	theme	ATP/ADP	182:188	arg1	translocator					190:201	an ATP/ADP translocator	179:201	an ATP/ADP translocator (NTT)	179:207	The importance of a plastidial soluble inorganic pyrophosphatase (psPPase) and an ATP/ADP translocator (NTT) for starch composition and tuber formation in potato (Solanum tuberosum) was evaluated by individual and simultaneous down-regulation of the corresponding endogenous genes.
29538769	6	51	theme	biosynthesis	1022:1033	arg1	intermediates					995:1007	intermediates	995:1007	intermediates of vitamin C biosynthesis	995:1033	Increased amounts of intermediates of vitamin C biosynthesis were also observed.
29538769	1	52	theme	genes	375:379	arg1	down-regulation					327:341	individual and simultaneous down-regulation	299:341	down-regulation	327:341	The importance of a plastidial soluble inorganic pyrophosphatase (psPPase) and an ATP/ADP translocator (NTT) for starch composition and tuber formation in potato (Solanum tuberosum) was evaluated by individual and simultaneous down-regulation of the corresponding endogenous genes.
29538769	8	53	theme	efficient	1387:1395	arg1	import					1401:1406	efficient ATP import	1387:1406	efficient ATP import	1387:1406	Additionally, it demonstrates that functional PPi hydrolysis in combination with efficient ATP import is essential for tuber formation and development.
29538769	2	54	theme	lines	427:431	arg1	content					401:407	Starch and amylose content	382:407	Starch and amylose content of the transgenic lines	382:431	Starch and amylose content of the transgenic lines were considerably lower, and granule size substantially smaller, with down-regulation of StpsPPase generating the most pronounced effects.
29538769	2	55	theme	pronounced	552:561	arg1	effects					563:569	the most pronounced effects	543:569	the most pronounced effects	543:569	Starch and amylose content of the transgenic lines were considerably lower, and granule size substantially smaller, with down-regulation of StpsPPase generating the most pronounced effects.
29538769	3	56	theme	Single-gene	572:582	arg1	down-regulation					584:598	Single-gene down-regulation	572:598	Single-gene down-regulation of either StpsPPase or StNTT	572:627	Single-gene down-regulation of either StpsPPase or StNTT resulted in increased tuber numbers per plant and higher fresh weight yield.
29538769	0	57	from	changes	51:57	arg1	potato					92:97	potato	92:97	potato	92:97	Inhibition of plastid PPase and NTT leads to major changes in starch and tuber formation in potato.
29538769	0	57	from	changes	51:57	arg1	starch					62:67	starch	62:67	starch	62:67	Inhibition of plastid PPase and NTT leads to major changes in starch and tuber formation in potato.
29538769	0	57	from	changes	51:57	arg1	formation					79:87	tuber formation	73:87	tuber formation	73:87	Inhibition of plastid PPase and NTT leads to major changes in starch and tuber formation in potato.
29538769	1	58	theme	translocator	190:201	arg1	importance					104:113	The importance	100:113	The importance of a plastidial soluble inorganic pyrophosphatase (psPPase) and an ATP/ADP translocator (NTT) for starch composition and tuber formation in potato (Solanum tuberosum)	100:280	The importance of a plastidial soluble inorganic pyrophosphatase (psPPase) and an ATP/ADP translocator (NTT) for starch composition and tuber formation in potato (Solanum tuberosum) was evaluated by individual and simultaneous down-regulation of the corresponding endogenous genes.
29538769	8	59	theme	tuber	1425:1429	arg1	formation					1431:1439	tuber formation	1425:1439	tuber formation	1425:1439	Additionally, it demonstrates that functional PPi hydrolysis in combination with efficient ATP import is essential for tuber formation and development.
29538769	3	60	theme	StNTT	623:627	arg1	down-regulation					584:598	Single-gene down-regulation	572:598	Single-gene down-regulation of either StpsPPase or StNTT	572:627	Single-gene down-regulation of either StpsPPase or StNTT resulted in increased tuber numbers per plant and higher fresh weight yield.
29538769	2	61	theme	granule	462:468	arg1	size					470:473	granule size	462:473	granule size	462:473	Starch and amylose content of the transgenic lines were considerably lower, and granule size substantially smaller, with down-regulation of StpsPPase generating the most pronounced effects.
29538769	5	62	from	increase	918:925	arg1	content					942:948	ADP-glucose content	930:948	ADP-glucose content of the StpsPPase lines	930:971	Analysis of metabolites revealed altered amounts of sugar intermediates, and a substantial increase in ADP-glucose content of the StpsPPase lines.
29538769	6	63	theme	vitamin	1012:1018	arg1	biosynthesis					1022:1033	vitamin C biosynthesis	1012:1033	vitamin C biosynthesis	1012:1033	Increased amounts of intermediates of vitamin C biosynthesis were also observed.
29538769	4	64	dep	few	794:796	arg1	a					792:792	a	792:792	a	792:792	In contrast, when both genes were inhibited simultaneously, some lines developed only a few, small and distorted tubers.
29538769	8	65	from	hydrolysis	1356:1365	arg1	combination					1370:1380	combination	1370:1380	combination with efficient ATP import	1370:1406	Additionally, it demonstrates that functional PPi hydrolysis in combination with efficient ATP import is essential for tuber formation and development.
29538769	5	66	theme	metabolites	839:849	arg1	Analysis					827:834	Analysis	827:834	Analysis of metabolites	827:849	Analysis of metabolites revealed altered amounts of sugar intermediates, and a substantial increase in ADP-glucose content of the StpsPPase lines.
29538769	0	67	theme	major	45:49	arg1	changes					51:57	major changes	45:57	major changes in starch and tuber formation in potato	45:97	Inhibition of plastid PPase and NTT leads to major changes in starch and tuber formation in potato.
29538769	3	68	theme	weight	692:697	arg1	yield					699:703	higher fresh weight yield	679:703	higher fresh weight yield	679:703	Single-gene down-regulation of either StpsPPase or StNTT resulted in increased tuber numbers per plant and higher fresh weight yield.
29538769	3	69	theme	StpsPPase	610:618	arg1	down-regulation					584:598	Single-gene down-regulation	572:598	Single-gene down-regulation of either StpsPPase or StNTT	572:627	Single-gene down-regulation of either StpsPPase or StNTT resulted in increased tuber numbers per plant and higher fresh weight yield.
29538769	6	70	theme	C	1020:1020	arg1	biosynthesis					1022:1033	vitamin C biosynthesis	1012:1033	vitamin C biosynthesis	1012:1033	Increased amounts of intermediates of vitamin C biosynthesis were also observed.
29538769	7	71	theme	additional	1210:1219	arg1	mechanism					1221:1229	no additional mechanism	1207:1229	no additional mechanism for consuming, hydrolysing, or exporting PPi	1207:1274	This study suggests that hydrolysis of pyrophosphate (PPi) by action of a psPPase is vital for functional starch accumulation in potato tubers and that no additional mechanism for consuming, hydrolysing, or exporting PPi exists in the studied tissue.
29538769	6	72	theme	intermediates	995:1007	arg1	amounts					984:990	Increased amounts	974:990	Increased amounts of intermediates of vitamin C biosynthesis	974:1033	Increased amounts of intermediates of vitamin C biosynthesis were also observed.
29538769	6	72	theme	intermediates	995:1007	arg1	intermediates					995:1007	intermediates	995:1007	intermediates of vitamin C biosynthesis	995:1033	Increased amounts of intermediates of vitamin C biosynthesis were also observed.
29749563	8	0	theme	significant	1202:1212	arg1	downregulation					1214:1227	no significant downregulation	1199:1227	no significant downregulation of intracellular Mn2+ content with Zn2+ supplementation	1199:1283	Measurement of total intracellular Zn2+ content, however, indicates no significant upregulation of total intracellular Zn2+ content and no significant downregulation of intracellular Mn2+ content with Zn2+ supplementation.
29749563	6	1	theme	Zn2+/Mn2+	858:866	arg1	ratio					868:872	Zn2+/Mn2+ ratio	858:872	Zn2+/Mn2+ ratio	858:872	This suggests that one possible explanation of the effect of Zn2+ may be reduced intracellular galactosyltransferase activity due to increase in Zn2+/Mn2+ ratio.
29749563	12	2	theme	element	1845:1851	arg1	concentrations					1853:1866	trace element concentrations	1839:1866	trace element concentrations	1839:1866	This study also suggests the use of Mn2+ supplementation as a strategy to overcome the effect of lot-to-lot variability in trace element concentrations on galactosylation.
29749563	5	3	theme	Zn2+/Mn2+	696:704	arg1	ratio					706:710	Zn2+/Mn2+ ratio	696:710	Zn2+/Mn2+ ratio	696:710	Measurements of galactosyltransferase activity in the cell lysate show that activity decreases with increasing Zn2+/Mn2+ ratio.
29749563	10	4	theme	Zn2+	1476:1479	arg1	supplementation					1481:1495	Zn2+ supplementation	1476:1495	Zn2+ supplementation	1476:1495	In this case, Zn2+ supplementation would be expected to interfere with other known effects of Mn2+.
29749563	8	5	theme	Mn2+	1246:1249	arg1	content					1251:1257	intracellular Mn2+ content	1232:1257	intracellular Mn2+ content with Zn2+ supplementation	1232:1283	Measurement of total intracellular Zn2+ content, however, indicates no significant upregulation of total intracellular Zn2+ content and no significant downregulation of intracellular Mn2+ content with Zn2+ supplementation.
29749563	1	6	theme	glycoproteins	176:188	arg1	glycoform					151:159	glycoform	151:159	glycoform of recombinant glycoproteins	151:188	Trace element composition of culture medium can be altered to modulate glycoform of recombinant glycoproteins.
29749563	9	7	from	changes	1415:1421	arg1	distribution					1440:1451	intracellular distribution	1426:1451	intracellular distribution of Mn2+	1426:1459	One possible explanation could be that cellular detoxification response to higher extracellular Zn2+ concentration might lead to changes in intracellular distribution of Mn2+.
29749563	12	8	theme	lot-to-lot	1813:1822	arg1	variability					1824:1834	lot-to-lot variability	1813:1834	lot-to-lot variability in trace element concentrations	1813:1866	This study also suggests the use of Mn2+ supplementation as a strategy to overcome the effect of lot-to-lot variability in trace element concentrations on galactosylation.
29749563	6	9	theme	galactosyltransferase	808:828	arg1	activity					830:837	intracellular galactosyltransferase activity	794:837	intracellular galactosyltransferase activity	794:837	This suggests that one possible explanation of the effect of Zn2+ may be reduced intracellular galactosyltransferase activity due to increase in Zn2+/Mn2+ ratio.
29749563	11	10	theme	Mn2+	1632:1635	arg1	supplementation					1637:1651	Mn2+ supplementation	1632:1651	Mn2+ supplementation	1632:1651	Indeed, the previously reported increase in high mannose glycans upon Mn2+ supplementation in the absence of glucose is reversed by Zn2+ supplementation.
29749563	2	11	theme	IgG	300:302	arg1	galactosylation					269:283	galactosylation	269:283	galactosylation of recombinant IgG expressed in Chinese Hamster Ovary cells	269:343	In this study, we show that Zn2+ supplementation at or above 100 μM decreases galactosylation of recombinant IgG expressed in Chinese Hamster Ovary cells.
29749563	2	12	theme	Zn2+	219:222	arg1	supplementation					224:238	Zn2+ supplementation	219:238	Zn2+ supplementation at or above 100 μM	219:257	In this study, we show that Zn2+ supplementation at or above 100 μM decreases galactosylation of recombinant IgG expressed in Chinese Hamster Ovary cells.
29749563	4	13	theme	galactose	480:488	arg1	supplementation					461:475	supplementation	461:475	supplementation of galactose and uridine to the culture	461:515	This effect persists upon supplementation of galactose and uridine to the culture, indicating that it may not be due to reduced UDP-Gal availability.
29749563	9	14	theme	detoxification	1334:1347	arg1	response					1349:1356	cellular detoxification response	1325:1356	cellular detoxification response to higher extracellular Zn2+ concentration	1325:1399	One possible explanation could be that cellular detoxification response to higher extracellular Zn2+ concentration might lead to changes in intracellular distribution of Mn2+.
29749563	7	15	theme	Mn2+	1049:1052	arg1	content					1054:1060	intracellular Mn2+ content	1035:1060	intracellular Mn2+ content	1035:1060	Consistent with this, the decrease in galactosylation of IgG could be reversed by supplementation of Mn2+ (a cofactor of galactosyltransferase) which increases intracellular Mn2+ content.
29749563	8	16	with	content	1251:1257	arg1	supplementation					1269:1283	Zn2+ supplementation	1264:1283	Zn2+ supplementation	1264:1283	Measurement of total intracellular Zn2+ content, however, indicates no significant upregulation of total intracellular Zn2+ content and no significant downregulation of intracellular Mn2+ content with Zn2+ supplementation.
29749563	11	17	theme	reported	1585:1592	arg1	increase					1594:1601	the previously reported increase	1570:1601	the previously reported increase in high mannose glycans upon Mn2+ supplementation in the absence of glucose	1570:1677	Indeed, the previously reported increase in high mannose glycans upon Mn2+ supplementation in the absence of glucose is reversed by Zn2+ supplementation.
29749563	1	18	gly	glycoform	151:159	arg1	glycoproteins					176:188	recombinant glycoproteins	164:188	recombinant glycoproteins	164:188	Trace element composition of culture medium can be altered to modulate glycoform of recombinant glycoproteins.
29749563	5	19	from	Measurements	585:596	arg1	lysate					644:649	the cell lysate	635:649	the cell lysate	635:649	Measurements of galactosyltransferase activity in the cell lysate show that activity decreases with increasing Zn2+/Mn2+ ratio.
29749563	0	20	theme	CHO	69:71	arg1	cells					73:77	CHO cells	69:77	CHO cells	69:77	Zinc supplementation decreases galactosylation of recombinant IgG in CHO cells.
29749563	9	21	theme	extracellular	1368:1380	arg1	concentration					1387:1399	higher extracellular Zn2+ concentration	1361:1399	higher extracellular Zn2+ concentration	1361:1399	One possible explanation could be that cellular detoxification response to higher extracellular Zn2+ concentration might lead to changes in intracellular distribution of Mn2+.
29749563	11	22	theme	mannose	1611:1617	arg1	glycans					1619:1625	high mannose glycans	1606:1625	high mannose glycans	1606:1625	Indeed, the previously reported increase in high mannose glycans upon Mn2+ supplementation in the absence of glucose is reversed by Zn2+ supplementation.
29749563	5	23	theme	activity	623:630	arg1	Measurements					585:596	Measurements	585:596	Measurements of galactosyltransferase activity in the cell lysate	585:649	Measurements of galactosyltransferase activity in the cell lysate show that activity decreases with increasing Zn2+/Mn2+ ratio.
29749563	10	24	theme	other	1533:1537	arg1	effects					1545:1551	other known effects	1533:1551	other known effects of Mn2+	1533:1559	In this case, Zn2+ supplementation would be expected to interfere with other known effects of Mn2+.
29749563	8	25	theme	intracellular	1084:1096	arg1	content					1103:1109	total intracellular Zn2+ content	1078:1109	total intracellular Zn2+ content	1078:1109	Measurement of total intracellular Zn2+ content, however, indicates no significant upregulation of total intracellular Zn2+ content and no significant downregulation of intracellular Mn2+ content with Zn2+ supplementation.
29749563	4	26	theme	uridine	494:500	arg1	supplementation					461:475	supplementation	461:475	supplementation of galactose and uridine to the culture	461:515	This effect persists upon supplementation of galactose and uridine to the culture, indicating that it may not be due to reduced UDP-Gal availability.
29749563	6	27	theme	possible	736:743	arg1	explanation					745:755	one possible explanation	732:755	one possible explanation of the effect of Zn2+	732:777	This suggests that one possible explanation of the effect of Zn2+ may be reduced intracellular galactosyltransferase activity due to increase in Zn2+/Mn2+ ratio.
29749563	8	28	theme	content	1103:1109	arg1	Measurement					1063:1073	Measurement	1063:1073	Measurement of total intracellular Zn2+ content	1063:1109	Measurement of total intracellular Zn2+ content, however, indicates no significant upregulation of total intracellular Zn2+ content and no significant downregulation of intracellular Mn2+ content with Zn2+ supplementation.
29749563	7	29	theme	Mn2+	976:979	arg1	supplementation					957:971	supplementation	957:971	supplementation of Mn2+ (a cofactor of galactosyltransferase) which increases intracellular Mn2+ content	957:1060	Consistent with this, the decrease in galactosylation of IgG could be reversed by supplementation of Mn2+ (a cofactor of galactosyltransferase) which increases intracellular Mn2+ content.
29749563	1	30	theme	Trace	80:84	arg1	composition					94:104	Trace element composition	80:104	Trace element composition of culture medium	80:122	Trace element composition of culture medium can be altered to modulate glycoform of recombinant glycoproteins.
29749563	12	31	theme	supplementation	1757:1771	arg1	strategy					1778:1785	a strategy	1776:1785	a strategy to overcome the effect of lot-to-lot variability in trace element concentrations on galactosylation	1776:1885	This study also suggests the use of Mn2+ supplementation as a strategy to overcome the effect of lot-to-lot variability in trace element concentrations on galactosylation.
29749563	12	31	theme	supplementation	1757:1771	arg1	use					1745:1747	the use	1741:1747	the use of Mn2+ supplementation	1741:1771	This study also suggests the use of Mn2+ supplementation as a strategy to overcome the effect of lot-to-lot variability in trace element concentrations on galactosylation.
29749563	10	32	theme	Mn2+	1556:1559	arg1	effects					1545:1551	other known effects	1533:1551	other known effects of Mn2+	1533:1559	In this case, Zn2+ supplementation would be expected to interfere with other known effects of Mn2+.
29749563	0	33	theme	Zinc	0:3	arg1	supplementation					5:19	Zinc supplementation	0:19	Zinc supplementation	0:19	Zinc supplementation decreases galactosylation of recombinant IgG in CHO cells.
29749563	9	34	theme	intracellular	1426:1438	arg1	distribution					1440:1451	intracellular distribution	1426:1451	intracellular distribution of Mn2+	1426:1459	One possible explanation could be that cellular detoxification response to higher extracellular Zn2+ concentration might lead to changes in intracellular distribution of Mn2+.
29749563	1	35	theme	culture	109:115	arg1	medium					117:122	culture medium	109:122	culture medium	109:122	Trace element composition of culture medium can be altered to modulate glycoform of recombinant glycoproteins.
29749563	4	36	theme	UDP-Gal	563:569	arg1	availability					571:582	reduced UDP-Gal availability	555:582	reduced UDP-Gal availability	555:582	This effect persists upon supplementation of galactose and uridine to the culture, indicating that it may not be due to reduced UDP-Gal availability.
29749563	8	37	theme	significant	1134:1144	arg1	upregulation					1146:1157	no significant upregulation	1131:1157	no significant upregulation of total intracellular Zn2+ content	1131:1193	Measurement of total intracellular Zn2+ content, however, indicates no significant upregulation of total intracellular Zn2+ content and no significant downregulation of intracellular Mn2+ content with Zn2+ supplementation.
29749563	7	38	with	Consistent	875:884	arg1	this					891:894	this	891:894	this	891:894	Consistent with this, the decrease in galactosylation of IgG could be reversed by supplementation of Mn2+ (a cofactor of galactosyltransferase) which increases intracellular Mn2+ content.
29749563	3	39	theme	reduced	393:399	arg1	expression					423:432	reduced galactosyltransferase expression	393:432	reduced galactosyltransferase expression	393:432	This decrease in galactosylation is not due to reduced galactosyltransferase expression.
29749563	0	40	theme	IgG	62:64	arg1	galactosylation					31:45	galactosylation	31:45	galactosylation of recombinant IgG in CHO cells	31:77	Zinc supplementation decreases galactosylation of recombinant IgG in CHO cells.
29749563	6	41	theme	Zn2+	774:777	arg1	effect					764:769	the effect	760:769	the effect of Zn2+	760:777	This suggests that one possible explanation of the effect of Zn2+ may be reduced intracellular galactosyltransferase activity due to increase in Zn2+/Mn2+ ratio.
29749563	2	42	theme	Ovary	333:337	arg1	cells					339:343	Chinese Hamster Ovary cells	317:343	Chinese Hamster Ovary cells	317:343	In this study, we show that Zn2+ supplementation at or above 100 μM decreases galactosylation of recombinant IgG expressed in Chinese Hamster Ovary cells.
29749563	8	43	theme	intracellular	1168:1180	arg1	content					1187:1193	total intracellular Zn2+ content	1162:1193	total intracellular Zn2+ content	1162:1193	Measurement of total intracellular Zn2+ content, however, indicates no significant upregulation of total intracellular Zn2+ content and no significant downregulation of intracellular Mn2+ content with Zn2+ supplementation.
29749563	6	44	theme	effect	764:769	arg1	explanation					745:755	one possible explanation	732:755	one possible explanation of the effect of Zn2+	732:777	This suggests that one possible explanation of the effect of Zn2+ may be reduced intracellular galactosyltransferase activity due to increase in Zn2+/Mn2+ ratio.
29749563	8	45	theme	content	1187:1193	arg1	upregulation					1146:1157	no significant upregulation	1131:1157	no significant upregulation of total intracellular Zn2+ content	1131:1193	Measurement of total intracellular Zn2+ content, however, indicates no significant upregulation of total intracellular Zn2+ content and no significant downregulation of intracellular Mn2+ content with Zn2+ supplementation.
29749563	8	45	theme	content	1187:1193	arg1	downregulation					1214:1227	no significant downregulation	1199:1227	no significant downregulation of intracellular Mn2+ content with Zn2+ supplementation	1199:1283	Measurement of total intracellular Zn2+ content, however, indicates no significant upregulation of total intracellular Zn2+ content and no significant downregulation of intracellular Mn2+ content with Zn2+ supplementation.
29749563	1	46	gly	glycoproteins	176:188	arg1	glycoproteins					176:188	recombinant glycoproteins	164:188	recombinant glycoproteins	164:188	Trace element composition of culture medium can be altered to modulate glycoform of recombinant glycoproteins.
29749563	7	47	theme	IgG	932:934	arg1	galactosylation					913:927	galactosylation	913:927	galactosylation of IgG	913:934	Consistent with this, the decrease in galactosylation of IgG could be reversed by supplementation of Mn2+ (a cofactor of galactosyltransferase) which increases intracellular Mn2+ content.
29749563	2	48	theme	recombinant	288:298	arg1	IgG					300:302	recombinant IgG	288:302	recombinant IgG expressed in Chinese Hamster Ovary cells	288:343	In this study, we show that Zn2+ supplementation at or above 100 μM decreases galactosylation of recombinant IgG expressed in Chinese Hamster Ovary cells.
29749563	1	49	theme	recombinant	164:174	arg1	glycoproteins					176:188	recombinant glycoproteins	164:188	recombinant glycoproteins	164:188	Trace element composition of culture medium can be altered to modulate glycoform of recombinant glycoproteins.
29749563	8	50	theme	intracellular	1232:1244	arg1	content					1251:1257	intracellular Mn2+ content	1232:1257	intracellular Mn2+ content with Zn2+ supplementation	1232:1283	Measurement of total intracellular Zn2+ content, however, indicates no significant upregulation of total intracellular Zn2+ content and no significant downregulation of intracellular Mn2+ content with Zn2+ supplementation.
29749563	12	51	theme	variability	1824:1834	arg1	effect					1803:1808	the effect	1799:1808	the effect of lot-to-lot variability in trace element concentrations on galactosylation	1799:1885	This study also suggests the use of Mn2+ supplementation as a strategy to overcome the effect of lot-to-lot variability in trace element concentrations on galactosylation.
29749563	2	52	theme	Chinese	317:323	arg1	cells					339:343	Chinese Hamster Ovary cells	317:343	Chinese Hamster Ovary cells	317:343	In this study, we show that Zn2+ supplementation at or above 100 μM decreases galactosylation of recombinant IgG expressed in Chinese Hamster Ovary cells.
29749563	8	53	theme	content	1251:1257	arg1	upregulation					1146:1157	no significant upregulation	1131:1157	no significant upregulation of total intracellular Zn2+ content	1131:1193	Measurement of total intracellular Zn2+ content, however, indicates no significant upregulation of total intracellular Zn2+ content and no significant downregulation of intracellular Mn2+ content with Zn2+ supplementation.
29749563	8	53	theme	content	1251:1257	arg1	downregulation					1214:1227	no significant downregulation	1199:1227	no significant downregulation of intracellular Mn2+ content with Zn2+ supplementation	1199:1283	Measurement of total intracellular Zn2+ content, however, indicates no significant upregulation of total intracellular Zn2+ content and no significant downregulation of intracellular Mn2+ content with Zn2+ supplementation.
29749563	12	54	theme	trace	1839:1843	arg1	concentrations					1853:1866	trace element concentrations	1839:1866	trace element concentrations	1839:1866	This study also suggests the use of Mn2+ supplementation as a strategy to overcome the effect of lot-to-lot variability in trace element concentrations on galactosylation.
29749563	9	55	theme	possible	1290:1297	arg1	explanation					1299:1309	One possible explanation	1286:1309	One possible explanation	1286:1309	One possible explanation could be that cellular detoxification response to higher extracellular Zn2+ concentration might lead to changes in intracellular distribution of Mn2+.
29749563	6	56	theme	intracellular	794:806	arg1	activity					830:837	intracellular galactosyltransferase activity	794:837	intracellular galactosyltransferase activity	794:837	This suggests that one possible explanation of the effect of Zn2+ may be reduced intracellular galactosyltransferase activity due to increase in Zn2+/Mn2+ ratio.
29749563	8	57	theme	Zn2+	1264:1267	arg1	supplementation					1269:1283	Zn2+ supplementation	1264:1283	Zn2+ supplementation	1264:1283	Measurement of total intracellular Zn2+ content, however, indicates no significant upregulation of total intracellular Zn2+ content and no significant downregulation of intracellular Mn2+ content with Zn2+ supplementation.
29749563	3	58	from	decrease	351:358	arg1	galactosylation					363:377	galactosylation	363:377	galactosylation	363:377	This decrease in galactosylation is not due to reduced galactosyltransferase expression.
29749563	12	59	from	effect	1803:1808	arg1	concentrations					1853:1866	trace element concentrations	1839:1866	trace element concentrations	1839:1866	This study also suggests the use of Mn2+ supplementation as a strategy to overcome the effect of lot-to-lot variability in trace element concentrations on galactosylation.
29749563	12	59	from	effect	1803:1808	arg1	galactosylation					1871:1885	galactosylation	1871:1885	galactosylation	1871:1885	This study also suggests the use of Mn2+ supplementation as a strategy to overcome the effect of lot-to-lot variability in trace element concentrations on galactosylation.
29749563	9	60	theme	cellular	1325:1332	arg1	response					1349:1356	cellular detoxification response	1325:1356	cellular detoxification response to higher extracellular Zn2+ concentration	1325:1399	One possible explanation could be that cellular detoxification response to higher extracellular Zn2+ concentration might lead to changes in intracellular distribution of Mn2+.
29749563	1	61	theme	medium	117:122	arg1	composition					94:104	Trace element composition	80:104	Trace element composition of culture medium	80:122	Trace element composition of culture medium can be altered to modulate glycoform of recombinant glycoproteins.
29749563	9	62	theme	higher	1361:1366	arg1	concentration					1387:1399	higher extracellular Zn2+ concentration	1361:1399	higher extracellular Zn2+ concentration	1361:1399	One possible explanation could be that cellular detoxification response to higher extracellular Zn2+ concentration might lead to changes in intracellular distribution of Mn2+.
29749563	7	63	theme	intracellular	1035:1047	arg1	content					1054:1060	intracellular Mn2+ content	1035:1060	intracellular Mn2+ content	1035:1060	Consistent with this, the decrease in galactosylation of IgG could be reversed by supplementation of Mn2+ (a cofactor of galactosyltransferase) which increases intracellular Mn2+ content.
29749563	0	64	from	galactosylation	31:45	arg1	cells					73:77	CHO cells	69:77	CHO cells	69:77	Zinc supplementation decreases galactosylation of recombinant IgG in CHO cells.
29749563	11	65	theme	high	1606:1609	arg1	glycans					1619:1625	high mannose glycans	1606:1625	high mannose glycans	1606:1625	Indeed, the previously reported increase in high mannose glycans upon Mn2+ supplementation in the absence of glucose is reversed by Zn2+ supplementation.
29749563	9	66	theme	Zn2+	1382:1385	arg1	concentration					1387:1399	higher extracellular Zn2+ concentration	1361:1399	higher extracellular Zn2+ concentration	1361:1399	One possible explanation could be that cellular detoxification response to higher extracellular Zn2+ concentration might lead to changes in intracellular distribution of Mn2+.
29749563	8	67	theme	total	1078:1082	arg1	content					1103:1109	total intracellular Zn2+ content	1078:1109	total intracellular Zn2+ content	1078:1109	Measurement of total intracellular Zn2+ content, however, indicates no significant upregulation of total intracellular Zn2+ content and no significant downregulation of intracellular Mn2+ content with Zn2+ supplementation.
29749563	10	68	theme	known	1539:1543	arg1	effects					1545:1551	other known effects	1533:1551	other known effects of Mn2+	1533:1559	In this case, Zn2+ supplementation would be expected to interfere with other known effects of Mn2+.
29749563	7	69	dep	Mn2+	976:979	arg1	cofactor					984:991	a cofactor	982:991	a cofactor of galactosyltransferase	982:1016	Consistent with this, the decrease in galactosylation of IgG could be reversed by supplementation of Mn2+ (a cofactor of galactosyltransferase) which increases intracellular Mn2+ content.
29749563	8	70	theme	Zn2+	1098:1101	arg1	content					1103:1109	total intracellular Zn2+ content	1078:1109	total intracellular Zn2+ content	1078:1109	Measurement of total intracellular Zn2+ content, however, indicates no significant upregulation of total intracellular Zn2+ content and no significant downregulation of intracellular Mn2+ content with Zn2+ supplementation.
29749563	7	71	theme	galactosyltransferase	996:1016	arg1	cofactor					984:991	a cofactor	982:991	a cofactor of galactosyltransferase	982:1016	Consistent with this, the decrease in galactosylation of IgG could be reversed by supplementation of Mn2+ (a cofactor of galactosyltransferase) which increases intracellular Mn2+ content.
29749563	11	72	from	increase	1594:1601	arg1	glycans					1619:1625	high mannose glycans	1606:1625	high mannose glycans	1606:1625	Indeed, the previously reported increase in high mannose glycans upon Mn2+ supplementation in the absence of glucose is reversed by Zn2+ supplementation.
29749563	11	72	from	increase	1594:1601	arg1	absence					1660:1666	the absence	1656:1666	the absence of glucose	1656:1677	Indeed, the previously reported increase in high mannose glycans upon Mn2+ supplementation in the absence of glucose is reversed by Zn2+ supplementation.
29749563	5	73	theme	cell	639:642	arg1	lysate					644:649	the cell lysate	635:649	the cell lysate	635:649	Measurements of galactosyltransferase activity in the cell lysate show that activity decreases with increasing Zn2+/Mn2+ ratio.
29749563	6	74	from	increase	846:853	arg1	ratio					868:872	Zn2+/Mn2+ ratio	858:872	Zn2+/Mn2+ ratio	858:872	This suggests that one possible explanation of the effect of Zn2+ may be reduced intracellular galactosyltransferase activity due to increase in Zn2+/Mn2+ ratio.
29749563	1	75	theme	element	86:92	arg1	composition					94:104	Trace element composition	80:104	Trace element composition of culture medium	80:122	Trace element composition of culture medium can be altered to modulate glycoform of recombinant glycoproteins.
29749563	12	76	theme	Mn2+	1752:1755	arg1	supplementation					1757:1771	Mn2+ supplementation	1752:1771	Mn2+ supplementation	1752:1771	This study also suggests the use of Mn2+ supplementation as a strategy to overcome the effect of lot-to-lot variability in trace element concentrations on galactosylation.
29749563	12	77	from	concentrations	1853:1866	arg1	effect					1803:1808	the effect	1799:1808	the effect of lot-to-lot variability in trace element concentrations on galactosylation	1799:1885	This study also suggests the use of Mn2+ supplementation as a strategy to overcome the effect of lot-to-lot variability in trace element concentrations on galactosylation.
29749563	4	78	theme	reduced	555:561	arg1	availability					571:582	reduced UDP-Gal availability	555:582	reduced UDP-Gal availability	555:582	This effect persists upon supplementation of galactose and uridine to the culture, indicating that it may not be due to reduced UDP-Gal availability.
29749563	5	79	theme	galactosyltransferase	601:621	arg1	activity					623:630	galactosyltransferase activity	601:630	galactosyltransferase activity	601:630	Measurements of galactosyltransferase activity in the cell lysate show that activity decreases with increasing Zn2+/Mn2+ ratio.
29749563	0	80	theme	recombinant	50:60	arg1	IgG					62:64	recombinant IgG	50:64	recombinant IgG	50:64	Zinc supplementation decreases galactosylation of recombinant IgG in CHO cells.
29749563	9	81	theme	Mn2+	1456:1459	arg1	distribution					1440:1451	intracellular distribution	1426:1451	intracellular distribution of Mn2+	1426:1459	One possible explanation could be that cellular detoxification response to higher extracellular Zn2+ concentration might lead to changes in intracellular distribution of Mn2+.
29749563	12	82	from	variability	1824:1834	arg1	concentrations					1853:1866	trace element concentrations	1839:1866	trace element concentrations	1839:1866	This study also suggests the use of Mn2+ supplementation as a strategy to overcome the effect of lot-to-lot variability in trace element concentrations on galactosylation.
29749563	8	83	theme	total	1162:1166	arg1	content					1187:1193	total intracellular Zn2+ content	1162:1193	total intracellular Zn2+ content	1162:1193	Measurement of total intracellular Zn2+ content, however, indicates no significant upregulation of total intracellular Zn2+ content and no significant downregulation of intracellular Mn2+ content with Zn2+ supplementation.
29749563	3	84	theme	galactosyltransferase	401:421	arg1	expression					423:432	reduced galactosyltransferase expression	393:432	reduced galactosyltransferase expression	393:432	This decrease in galactosylation is not due to reduced galactosyltransferase expression.
29749563	11	85	theme	glucose	1671:1677	arg1	absence					1660:1666	the absence	1656:1666	the absence of glucose	1656:1677	Indeed, the previously reported increase in high mannose glycans upon Mn2+ supplementation in the absence of glucose is reversed by Zn2+ supplementation.
29749563	8	86	theme	Zn2+	1182:1185	arg1	content					1187:1193	total intracellular Zn2+ content	1162:1193	total intracellular Zn2+ content	1162:1193	Measurement of total intracellular Zn2+ content, however, indicates no significant upregulation of total intracellular Zn2+ content and no significant downregulation of intracellular Mn2+ content with Zn2+ supplementation.
29749563	2	87	theme	Hamster	325:331	arg1	cells					339:343	Chinese Hamster Ovary cells	317:343	Chinese Hamster Ovary cells	317:343	In this study, we show that Zn2+ supplementation at or above 100 μM decreases galactosylation of recombinant IgG expressed in Chinese Hamster Ovary cells.
29749563	7	88	from	decrease	901:908	arg1	galactosylation					913:927	galactosylation	913:927	galactosylation of IgG	913:934	Consistent with this, the decrease in galactosylation of IgG could be reversed by supplementation of Mn2+ (a cofactor of galactosyltransferase) which increases intracellular Mn2+ content.
29749563	11	89	theme	Zn2+	1694:1697	arg1	supplementation					1699:1713	Zn2+ supplementation	1694:1713	Zn2+ supplementation	1694:1713	Indeed, the previously reported increase in high mannose glycans upon Mn2+ supplementation in the absence of glucose is reversed by Zn2+ supplementation.
31177026	9	0	theme	Klason	1411:1416	arg1	lignin					1418:1423	the Klason lignin	1407:1423	the Klason lignin	1407:1423	The ratio of the non-hydrolysable part to the Klason lignin increased from 1.3 to 3.3 with increasing auxin concentrations in the substrate.
31177026	10	1	theme	turgor	1648:1653	arg1	pressure					1655:1662	turgor pressure	1648:1662	turgor pressure	1648:1662	This may be related to the deposition of polysaccharides and lignin in the cell wall, which help maintain the specific tensile stress of, and turgor pressure on, the cell walls.
31177026	10	2	theme	lignin	1567:1572	arg1	deposition					1533:1542	the deposition	1529:1542	the deposition	1529:1542	This may be related to the deposition of polysaccharides and lignin in the cell wall, which help maintain the specific tensile stress of, and turgor pressure on, the cell walls.
31177026	2	3	theme	maize	227:231	arg1	plants					233:238	maize plants	227:238	maize plants	227:238	The effects of auxin IBA (indole-3-butyric acid) during 10-days on maize plants growth in controlled conditions (hydroponic, 16-h photoperiod, 70% humidity, 25/20 °C temperature), depended on its concentration in the substrate.
31177026	2	4	theme	auxin	175:179	arg1	IBA					181:183	auxin IBA	175:183	auxin IBA (indole-3-butyric acid)	175:207	The effects of auxin IBA (indole-3-butyric acid) during 10-days on maize plants growth in controlled conditions (hydroponic, 16-h photoperiod, 70% humidity, 25/20 °C temperature), depended on its concentration in the substrate.
31177026	2	4	theme	auxin	175:179	arg1	acid					203:206	indole-3-butyric acid	186:206	indole-3-butyric acid	186:206	The effects of auxin IBA (indole-3-butyric acid) during 10-days on maize plants growth in controlled conditions (hydroponic, 16-h photoperiod, 70% humidity, 25/20 °C temperature), depended on its concentration in the substrate.
31177026	4	5	theme	apoplasmic	702:711	arg1	barriers					713:720	apoplasmic barriers	702:720	apoplasmic barriers	702:720	A low concentration (10-11 M) of IBA stimulated root growth but affected neither the development of apoplasmic barriers, nor the amount of lignin.
31177026	7	6	from	changes	1077:1083	arg1	composition					1092:1102	the composition	1088:1102	the composition of the PF III fraction (lignocellulosic complex) of the cell wall	1088:1168	We found the most significant changes in the composition of the PF III fraction (lignocellulosic complex) of the cell wall.
31177026	2	7	theme	10-days	216:222	arg1	growth					240:245	10-days on maize plants growth	216:245	10-days on maize plants growth in controlled conditions (hydroponic, 16-h photoperiod, 70% humidity, 25/20 °C temperature)	216:337	The effects of auxin IBA (indole-3-butyric acid) during 10-days on maize plants growth in controlled conditions (hydroponic, 16-h photoperiod, 70% humidity, 25/20 °C temperature), depended on its concentration in the substrate.
31177026	1	8	theme	cell	137:140	arg1	growth					152:157	cell and plant growth	137:157	growth	152:157	Auxin is one of the crucial plant hormones which stimulates and controls cell and plant growth.
31177026	3	9	from	roots	595:599	arg1	lignin					585:590	lignin	585:590	lignin in roots	585:599	A high concentration (10-7 M) of IBA inhibited root growth, evoked the development of apoplasmic barriers (Casparian bands and suberin lamellae) closer to the root apex, and elevated the amount of lignin in roots.
31177026	3	9	from	roots	595:599	arg1	amount					575:580	the amount	571:580	the amount of lignin in roots	571:599	A high concentration (10-7 M) of IBA inhibited root growth, evoked the development of apoplasmic barriers (Casparian bands and suberin lamellae) closer to the root apex, and elevated the amount of lignin in roots.
31177026	2	10	theme	controlled	250:259	arg1	conditions					261:270	controlled conditions	250:270	controlled conditions (hydroponic, 16-h photoperiod, 70% humidity, 25/20 °C temperature)	250:337	The effects of auxin IBA (indole-3-butyric acid) during 10-days on maize plants growth in controlled conditions (hydroponic, 16-h photoperiod, 70% humidity, 25/20 °C temperature), depended on its concentration in the substrate.
31177026	1	11	theme	plant	146:150	arg1	growth					152:157	cell and plant growth	137:157	growth	152:157	Auxin is one of the crucial plant hormones which stimulates and controls cell and plant growth.
31177026	6	12	theme	Plant	896:900	arg1	walls					907:911	Plant cell walls	896:911	Plant cell walls as cell structures	896:930	Plant cell walls as cell structures ensure cell enlargement and plant growth, and have to react to auxin stimulus by modification of their components.
31177026	3	13	from	amount	575:580	arg1	roots					595:599	roots	595:599	roots	595:599	A high concentration (10-7 M) of IBA inhibited root growth, evoked the development of apoplasmic barriers (Casparian bands and suberin lamellae) closer to the root apex, and elevated the amount of lignin in roots.
31177026	2	14	theme	IBA	181:183	arg1	effects					164:170	The effects	160:170	The effects	160:170	The effects of auxin IBA (indole-3-butyric acid) during 10-days on maize plants growth in controlled conditions (hydroponic, 16-h photoperiod, 70% humidity, 25/20 °C temperature), depended on its concentration in the substrate.
31177026	8	15	theme	fraction	1248:1255	arg1	components					1229:1238	all three components	1219:1238	all three components of this fraction - Klason lignin and both the by acid (2 M TFA) non-hydrolysable and the hydrolysable parts of this complex	1219:1362	The presence of auxin in the substrate affected all three components of this fraction - Klason lignin and both the by acid (2 M TFA) non-hydrolysable and the hydrolysable parts of this complex.
31177026	6	16	theme	cell	939:942	arg1	enlargement					944:954	cell enlargement	939:954	cell enlargement	939:954	Plant cell walls as cell structures ensure cell enlargement and plant growth, and have to react to auxin stimulus by modification of their components.
31177026	3	17	from	lignin	585:590	arg1	roots					595:599	roots	595:599	roots	595:599	A high concentration (10-7 M) of IBA inhibited root growth, evoked the development of apoplasmic barriers (Casparian bands and suberin lamellae) closer to the root apex, and elevated the amount of lignin in roots.
31177026	5	18	theme	10-8	760:763	arg1	M					765:765	M	765:765	M	765:765	Auxin in a 10-8 M concentration influenced the root growth to a minimal extent compare to the control, and it was the non-effective concentration.
31177026	8	19	theme	Klason	1259:1264	arg1	lignin					1266:1271	Klason lignin	1259:1271	Klason lignin	1259:1271	The presence of auxin in the substrate affected all three components of this fraction - Klason lignin and both the by acid (2 M TFA) non-hydrolysable and the hydrolysable parts of this complex.
31177026	7	20	theme	cell	1160:1163	arg1	wall					1165:1168	the cell wall	1156:1168	the cell wall	1156:1168	We found the most significant changes in the composition of the PF III fraction (lignocellulosic complex) of the cell wall.
31177026	2	21	dep	conditions	261:270	arg1	humidity					307:314	70% humidity	303:314	70% humidity	303:314	The effects of auxin IBA (indole-3-butyric acid) during 10-days on maize plants growth in controlled conditions (hydroponic, 16-h photoperiod, 70% humidity, 25/20 °C temperature), depended on its concentration in the substrate.
31177026	2	21	dep	conditions	261:270	arg1	temperature					326:336	25/20 °C temperature	317:336	25/20 °C temperature	317:336	The effects of auxin IBA (indole-3-butyric acid) during 10-days on maize plants growth in controlled conditions (hydroponic, 16-h photoperiod, 70% humidity, 25/20 °C temperature), depended on its concentration in the substrate.
31177026	2	21	dep	conditions	261:270	arg1	photoperiod					290:300	hydroponic, 16-h photoperiod	273:300	hydroponic, 16-h photoperiod	273:300	The effects of auxin IBA (indole-3-butyric acid) during 10-days on maize plants growth in controlled conditions (hydroponic, 16-h photoperiod, 70% humidity, 25/20 °C temperature), depended on its concentration in the substrate.
31177026	1	22	theme	plant	92:96	arg1	hormones					98:105	the crucial plant hormones	80:105	the crucial plant hormones which stimulates and controls cell and plant growth	80:157	Auxin is one of the crucial plant hormones which stimulates and controls cell and plant growth.
31177026	1	23	theme	crucial	84:90	arg1	hormones					98:105	the crucial plant hormones	80:105	the crucial plant hormones which stimulates and controls cell and plant growth	80:157	Auxin is one of the crucial plant hormones which stimulates and controls cell and plant growth.
31177026	0	24	theme	maize	41:45	arg1	composition					26:36	the composition	22:36	the composition of maize	22:45	The effects of IBA on the composition of maize root cell walls.
31177026	10	25	theme	cell	1581:1584	arg1	wall					1586:1589	the cell wall	1577:1589	the cell wall	1577:1589	This may be related to the deposition of polysaccharides and lignin in the cell wall, which help maintain the specific tensile stress of, and turgor pressure on, the cell walls.
31177026	10	26	from	deposition	1533:1542	arg1	wall					1586:1589	the cell wall	1577:1589	the cell wall	1577:1589	This may be related to the deposition of polysaccharides and lignin in the cell wall, which help maintain the specific tensile stress of, and turgor pressure on, the cell walls.
31177026	0	27	theme	cell	52:55	arg1	walls					57:61	cell walls	52:61	cell walls	52:61	The effects of IBA on the composition of maize root cell walls.
31177026	5	28	from	Auxin	749:753	arg1	concentration					767:779	a 10-8 M concentration	758:779	a 10-8 M concentration	758:779	Auxin in a 10-8 M concentration influenced the root growth to a minimal extent compare to the control, and it was the non-effective concentration.
31177026	10	29	from	polysaccharides	1547:1561	arg1	wall					1586:1589	the cell wall	1577:1589	the cell wall	1577:1589	This may be related to the deposition of polysaccharides and lignin in the cell wall, which help maintain the specific tensile stress of, and turgor pressure on, the cell walls.
31177026	10	30	from	lignin	1567:1572	arg1	wall					1586:1589	the cell wall	1577:1589	the cell wall	1577:1589	This may be related to the deposition of polysaccharides and lignin in the cell wall, which help maintain the specific tensile stress of, and turgor pressure on, the cell walls.
31177026	4	31	theme	IBA	635:637	arg1	concentration					608:620	A low concentration	602:620	A low concentration (10-11 M) of IBA	602:637	A low concentration (10-11 M) of IBA stimulated root growth but affected neither the development of apoplasmic barriers, nor the amount of lignin.
31177026	4	31	theme	IBA	635:637	arg1	M					629:629	10-11 M	623:629	10-11 M	623:629	A low concentration (10-11 M) of IBA stimulated root growth but affected neither the development of apoplasmic barriers, nor the amount of lignin.
31177026	2	32	dep	hydroponic	273:282	arg1	16-h					285:288	16-h	285:288	16-h	285:288	The effects of auxin IBA (indole-3-butyric acid) during 10-days on maize plants growth in controlled conditions (hydroponic, 16-h photoperiod, 70% humidity, 25/20 °C temperature), depended on its concentration in the substrate.
31177026	2	33	theme	indole-3-butyric	186:201	arg1	IBA					181:183	auxin IBA	175:183	auxin IBA (indole-3-butyric acid)	175:207	The effects of auxin IBA (indole-3-butyric acid) during 10-days on maize plants growth in controlled conditions (hydroponic, 16-h photoperiod, 70% humidity, 25/20 °C temperature), depended on its concentration in the substrate.
31177026	2	33	theme	indole-3-butyric	186:201	arg1	acid					203:206	indole-3-butyric acid	186:206	indole-3-butyric acid	186:206	The effects of auxin IBA (indole-3-butyric acid) during 10-days on maize plants growth in controlled conditions (hydroponic, 16-h photoperiod, 70% humidity, 25/20 °C temperature), depended on its concentration in the substrate.
31177026	10	34	from	wall	1586:1589	arg1	deposition					1533:1542	the deposition	1529:1542	the deposition	1529:1542	This may be related to the deposition of polysaccharides and lignin in the cell wall, which help maintain the specific tensile stress of, and turgor pressure on, the cell walls.
31177026	7	35	theme	lignocellulosic	1128:1142	arg1	fraction					1118:1125	the PF III fraction	1107:1125	the PF III fraction (lignocellulosic complex) of the cell wall	1107:1168	We found the most significant changes in the composition of the PF III fraction (lignocellulosic complex) of the cell wall.
31177026	7	35	theme	lignocellulosic	1128:1142	arg1	complex					1144:1150	lignocellulosic complex	1128:1150	lignocellulosic complex	1128:1150	We found the most significant changes in the composition of the PF III fraction (lignocellulosic complex) of the cell wall.
31177026	7	36	theme	fraction	1118:1125	arg1	composition					1092:1102	the composition	1088:1102	the composition of the PF III fraction (lignocellulosic complex) of the cell wall	1088:1168	We found the most significant changes in the composition of the PF III fraction (lignocellulosic complex) of the cell wall.
31177026	8	37	theme	hydrolysable	1329:1340	arg1	parts					1342:1346	the hydrolysable parts	1325:1346	the hydrolysable parts of this complex	1325:1362	The presence of auxin in the substrate affected all three components of this fraction - Klason lignin and both the by acid (2 M TFA) non-hydrolysable and the hydrolysable parts of this complex.
31177026	3	38	theme	root	547:550	arg1	apex					552:555	the root apex	543:555	the root apex	543:555	A high concentration (10-7 M) of IBA inhibited root growth, evoked the development of apoplasmic barriers (Casparian bands and suberin lamellae) closer to the root apex, and elevated the amount of lignin in roots.
31177026	7	39	theme	PF	1111:1112	arg1	fraction					1118:1125	the PF III fraction	1107:1125	the PF III fraction (lignocellulosic complex) of the cell wall	1107:1168	We found the most significant changes in the composition of the PF III fraction (lignocellulosic complex) of the cell wall.
31177026	7	39	theme	PF	1111:1112	arg1	complex					1144:1150	lignocellulosic complex	1128:1150	lignocellulosic complex	1128:1150	We found the most significant changes in the composition of the PF III fraction (lignocellulosic complex) of the cell wall.
31177026	6	40	theme	plant	960:964	arg1	growth					966:971	plant growth	960:971	plant growth	960:971	Plant cell walls as cell structures ensure cell enlargement and plant growth, and have to react to auxin stimulus by modification of their components.
31177026	8	41	theme	complex	1356:1362	arg1	lignin					1266:1271	Klason lignin	1259:1271	Klason lignin	1259:1271	The presence of auxin in the substrate affected all three components of this fraction - Klason lignin and both the by acid (2 M TFA) non-hydrolysable and the hydrolysable parts of this complex.
31177026	8	41	theme	complex	1356:1362	arg1	parts					1342:1346	the hydrolysable parts	1325:1346	the hydrolysable parts of this complex	1325:1362	The presence of auxin in the substrate affected all three components of this fraction - Klason lignin and both the by acid (2 M TFA) non-hydrolysable and the hydrolysable parts of this complex.
31177026	4	42	theme	root	650:653	arg1	growth					655:660	root growth	650:660	root growth	650:660	A low concentration (10-11 M) of IBA stimulated root growth but affected neither the development of apoplasmic barriers, nor the amount of lignin.
31177026	2	43	theme	hydroponic	273:282	arg1	humidity					307:314	70% humidity	303:314	70% humidity	303:314	The effects of auxin IBA (indole-3-butyric acid) during 10-days on maize plants growth in controlled conditions (hydroponic, 16-h photoperiod, 70% humidity, 25/20 °C temperature), depended on its concentration in the substrate.
31177026	2	43	theme	hydroponic	273:282	arg1	temperature					326:336	25/20 °C temperature	317:336	25/20 °C temperature	317:336	The effects of auxin IBA (indole-3-butyric acid) during 10-days on maize plants growth in controlled conditions (hydroponic, 16-h photoperiod, 70% humidity, 25/20 °C temperature), depended on its concentration in the substrate.
31177026	2	43	theme	hydroponic	273:282	arg1	photoperiod					290:300	hydroponic, 16-h photoperiod	273:300	hydroponic, 16-h photoperiod	273:300	The effects of auxin IBA (indole-3-butyric acid) during 10-days on maize plants growth in controlled conditions (hydroponic, 16-h photoperiod, 70% humidity, 25/20 °C temperature), depended on its concentration in the substrate.
31177026	8	44	dep	non-hydrolysable	1304:1319	arg1	TFA					1299:1301	2 M TFA	1295:1301	2 M TFA	1295:1301	The presence of auxin in the substrate affected all three components of this fraction - Klason lignin and both the by acid (2 M TFA) non-hydrolysable and the hydrolysable parts of this complex.
31177026	8	44	dep	non-hydrolysable	1304:1319	arg1	the					1282:1284	the	1282:1284	the	1282:1284	The presence of auxin in the substrate affected all three components of this fraction - Klason lignin and both the by acid (2 M TFA) non-hydrolysable and the hydrolysable parts of this complex.
31177026	9	45	theme	part	1399:1402	arg1	ratio					1369:1373	The ratio	1365:1373	The ratio of the non-hydrolysable part to the Klason lignin	1365:1423	The ratio of the non-hydrolysable part to the Klason lignin increased from 1.3 to 3.3 with increasing auxin concentrations in the substrate.
31177026	10	46	theme	tensile	1625:1631	arg1	stress					1633:1638	the specific tensile stress	1612:1638	the specific tensile stress of	1612:1641	This may be related to the deposition of polysaccharides and lignin in the cell wall, which help maintain the specific tensile stress of, and turgor pressure on, the cell walls.
31177026	3	47	theme	IBA	421:423	arg1	concentration					395:407	A high concentration	388:407	A high concentration (10-7 M) of IBA	388:423	A high concentration (10-7 M) of IBA inhibited root growth, evoked the development of apoplasmic barriers (Casparian bands and suberin lamellae) closer to the root apex, and elevated the amount of lignin in roots.
31177026	3	47	theme	IBA	421:423	arg1	M					415:415	10-7 M	410:415	10-7 M	410:415	A high concentration (10-7 M) of IBA inhibited root growth, evoked the development of apoplasmic barriers (Casparian bands and suberin lamellae) closer to the root apex, and elevated the amount of lignin in roots.
31177026	3	48	theme	lignin	585:590	arg1	lignin					585:590	lignin	585:590	lignin in roots	585:599	A high concentration (10-7 M) of IBA inhibited root growth, evoked the development of apoplasmic barriers (Casparian bands and suberin lamellae) closer to the root apex, and elevated the amount of lignin in roots.
31177026	3	48	theme	lignin	585:590	arg1	amount					575:580	the amount	571:580	the amount of lignin in roots	571:599	A high concentration (10-7 M) of IBA inhibited root growth, evoked the development of apoplasmic barriers (Casparian bands and suberin lamellae) closer to the root apex, and elevated the amount of lignin in roots.
31177026	8	49	from	presence	1175:1182	arg1	substrate					1200:1208	the substrate	1196:1208	the substrate	1196:1208	The presence of auxin in the substrate affected all three components of this fraction - Klason lignin and both the by acid (2 M TFA) non-hydrolysable and the hydrolysable parts of this complex.
31177026	6	50	theme	cell	916:919	arg1	structures					921:930	cell structures	916:930	cell structures	916:930	Plant cell walls as cell structures ensure cell enlargement and plant growth, and have to react to auxin stimulus by modification of their components.
31177026	6	51	theme	auxin	995:999	arg1	stimulus					1001:1008	auxin stimulus	995:1008	auxin stimulus	995:1008	Plant cell walls as cell structures ensure cell enlargement and plant growth, and have to react to auxin stimulus by modification of their components.
31177026	10	52	theme	cell	1672:1675	arg1	walls					1677:1681	, the cell walls	1666:1681	walls	1677:1681	This may be related to the deposition of polysaccharides and lignin in the cell wall, which help maintain the specific tensile stress of, and turgor pressure on, the cell walls.
31177026	3	53	theme	root	435:438	arg1	growth					440:445	root growth	435:445	root growth	435:445	A high concentration (10-7 M) of IBA inhibited root growth, evoked the development of apoplasmic barriers (Casparian bands and suberin lamellae) closer to the root apex, and elevated the amount of lignin in roots.
31177026	3	54	dep	barriers	485:492	arg1	bands					505:509	Casparian bands	495:509	Casparian bands	495:509	A high concentration (10-7 M) of IBA inhibited root growth, evoked the development of apoplasmic barriers (Casparian bands and suberin lamellae) closer to the root apex, and elevated the amount of lignin in roots.
31177026	3	54	dep	barriers	485:492	arg1	lamellae					523:530	suberin lamellae	515:530	suberin lamellae	515:530	A high concentration (10-7 M) of IBA inhibited root growth, evoked the development of apoplasmic barriers (Casparian bands and suberin lamellae) closer to the root apex, and elevated the amount of lignin in roots.
31177026	10	55	theme	polysaccharides	1547:1561	arg1	deposition					1533:1542	the deposition	1529:1542	the deposition	1529:1542	This may be related to the deposition of polysaccharides and lignin in the cell wall, which help maintain the specific tensile stress of, and turgor pressure on, the cell walls.
31177026	4	56	theme	barriers	713:720	arg1	development					687:697	the development	683:697	the development of apoplasmic barriers	683:720	A low concentration (10-11 M) of IBA stimulated root growth but affected neither the development of apoplasmic barriers, nor the amount of lignin.
31177026	3	57	theme	apoplasmic	474:483	arg1	barriers					485:492	apoplasmic barriers	474:492	apoplasmic barriers (Casparian bands and suberin lamellae) closer to the root apex	474:555	A high concentration (10-7 M) of IBA inhibited root growth, evoked the development of apoplasmic barriers (Casparian bands and suberin lamellae) closer to the root apex, and elevated the amount of lignin in roots.
31177026	2	58	theme	%	305:305	arg1	humidity					307:314	70% humidity	303:314	70% humidity	303:314	The effects of auxin IBA (indole-3-butyric acid) during 10-days on maize plants growth in controlled conditions (hydroponic, 16-h photoperiod, 70% humidity, 25/20 °C temperature), depended on its concentration in the substrate.
31177026	2	58	theme	%	305:305	arg1	photoperiod					290:300	hydroponic, 16-h photoperiod	273:300	hydroponic, 16-h photoperiod	273:300	The effects of auxin IBA (indole-3-butyric acid) during 10-days on maize plants growth in controlled conditions (hydroponic, 16-h photoperiod, 70% humidity, 25/20 °C temperature), depended on its concentration in the substrate.
31177026	3	59	theme	Casparian	495:503	arg1	bands					505:509	Casparian bands	495:509	Casparian bands	495:509	A high concentration (10-7 M) of IBA inhibited root growth, evoked the development of apoplasmic barriers (Casparian bands and suberin lamellae) closer to the root apex, and elevated the amount of lignin in roots.
31177026	3	60	theme	barriers	485:492	arg1	development					459:469	the development	455:469	the development of apoplasmic barriers (Casparian bands and suberin lamellae) closer to the root apex	455:555	A high concentration (10-7 M) of IBA inhibited root growth, evoked the development of apoplasmic barriers (Casparian bands and suberin lamellae) closer to the root apex, and elevated the amount of lignin in roots.
31177026	8	61	dep	components	1229:1238	arg1	lignin					1266:1271	Klason lignin	1259:1271	Klason lignin	1259:1271	The presence of auxin in the substrate affected all three components of this fraction - Klason lignin and both the by acid (2 M TFA) non-hydrolysable and the hydrolysable parts of this complex.
31177026	8	61	dep	components	1229:1238	arg1	non-hydrolysable					1304:1319	non-hydrolysable	1304:1319	non-hydrolysable	1304:1319	The presence of auxin in the substrate affected all three components of this fraction - Klason lignin and both the by acid (2 M TFA) non-hydrolysable and the hydrolysable parts of this complex.
31177026	4	62	theme	lignin	741:746	arg1	lignin					741:746	lignin	741:746	lignin	741:746	A low concentration (10-11 M) of IBA stimulated root growth but affected neither the development of apoplasmic barriers, nor the amount of lignin.
31177026	4	62	theme	lignin	741:746	arg1	amount					731:736	the amount	727:736	the amount of lignin	727:746	A low concentration (10-11 M) of IBA stimulated root growth but affected neither the development of apoplasmic barriers, nor the amount of lignin.
31177026	6	63	theme	components	1035:1044	arg1	modification					1013:1024	modification	1013:1024	modification of their components	1013:1044	Plant cell walls as cell structures ensure cell enlargement and plant growth, and have to react to auxin stimulus by modification of their components.
31177026	5	64	dep	extent	821:826	arg1	compare					828:834	compare	828:834	compare to the control	828:849	Auxin in a 10-8 M concentration influenced the root growth to a minimal extent compare to the control, and it was the non-effective concentration.
31177026	5	65	theme	non-effective	867:879	arg1	concentration					881:893	the non-effective concentration	863:893	the non-effective concentration	863:893	Auxin in a 10-8 M concentration influenced the root growth to a minimal extent compare to the control, and it was the non-effective concentration.
31177026	5	65	theme	non-effective	867:879	arg1	it					856:857	it	856:857	it	856:857	Auxin in a 10-8 M concentration influenced the root growth to a minimal extent compare to the control, and it was the non-effective concentration.
31177026	5	66	theme	M	765:765	arg1	concentration					767:779	a 10-8 M concentration	758:779	a 10-8 M concentration	758:779	Auxin in a 10-8 M concentration influenced the root growth to a minimal extent compare to the control, and it was the non-effective concentration.
31177026	7	67	theme	wall	1165:1168	arg1	fraction					1118:1125	the PF III fraction	1107:1125	the PF III fraction (lignocellulosic complex) of the cell wall	1107:1168	We found the most significant changes in the composition of the PF III fraction (lignocellulosic complex) of the cell wall.
31177026	7	67	theme	wall	1165:1168	arg1	complex					1144:1150	lignocellulosic complex	1128:1150	lignocellulosic complex	1128:1150	We found the most significant changes in the composition of the PF III fraction (lignocellulosic complex) of the cell wall.
31177026	1	68	theme	hormones	98:105	arg1	hormones					98:105	the crucial plant hormones	80:105	the crucial plant hormones which stimulates and controls cell and plant growth	80:157	Auxin is one of the crucial plant hormones which stimulates and controls cell and plant growth.
31177026	1	68	theme	hormones	98:105	arg1	one					73:75	one	73:75	one	73:75	Auxin is one of the crucial plant hormones which stimulates and controls cell and plant growth.
31177026	2	69	from	concentration	356:368	arg1	substrate					377:385	the substrate	373:385	the substrate	373:385	The effects of auxin IBA (indole-3-butyric acid) during 10-days on maize plants growth in controlled conditions (hydroponic, 16-h photoperiod, 70% humidity, 25/20 °C temperature), depended on its concentration in the substrate.
31177026	3	70	theme	suberin	515:521	arg1	lamellae					523:530	suberin lamellae	515:530	suberin lamellae	515:530	A high concentration (10-7 M) of IBA inhibited root growth, evoked the development of apoplasmic barriers (Casparian bands and suberin lamellae) closer to the root apex, and elevated the amount of lignin in roots.
31177026	2	71	from	growth	240:245	arg1	conditions					261:270	controlled conditions	250:270	controlled conditions (hydroponic, 16-h photoperiod, 70% humidity, 25/20 °C temperature)	250:337	The effects of auxin IBA (indole-3-butyric acid) during 10-days on maize plants growth in controlled conditions (hydroponic, 16-h photoperiod, 70% humidity, 25/20 °C temperature), depended on its concentration in the substrate.
31177026	9	72	from	concentrations	1473:1486	arg1	substrate					1495:1503	the substrate	1491:1503	the substrate	1491:1503	The ratio of the non-hydrolysable part to the Klason lignin increased from 1.3 to 3.3 with increasing auxin concentrations in the substrate.
31177026	9	73	theme	auxin	1467:1471	arg1	concentrations					1473:1486	auxin concentrations	1467:1486	auxin concentrations in the substrate	1467:1503	The ratio of the non-hydrolysable part to the Klason lignin increased from 1.3 to 3.3 with increasing auxin concentrations in the substrate.
31177026	0	74	from	effects	4:10	arg1	composition					26:36	the composition	22:36	the composition of maize	22:45	The effects of IBA on the composition of maize root cell walls.
31177026	8	75	attach	presence	1175:1182	arg2	auxin					1187:1191	auxin	1187:1191	auxin	1187:1191	The presence of auxin in the substrate affected all three components of this fraction - Klason lignin and both the by acid (2 M TFA) non-hydrolysable and the hydrolysable parts of this complex.
31177026	8	75	attach	presence	1175:1182	arg1	substrate					1200:1208	the substrate	1196:1208	the substrate	1196:1208	The presence of auxin in the substrate affected all three components of this fraction - Klason lignin and both the by acid (2 M TFA) non-hydrolysable and the hydrolysable parts of this complex.
31177026	5	76	theme	minimal	813:819	arg1	extent					821:826	a minimal extent	811:826	a minimal extent compare to the control	811:849	Auxin in a 10-8 M concentration influenced the root growth to a minimal extent compare to the control, and it was the non-effective concentration.
31177026	2	77	theme	25/20 °C	317:324	arg1	temperature					326:336	25/20 °C temperature	317:336	25/20 °C temperature	317:336	The effects of auxin IBA (indole-3-butyric acid) during 10-days on maize plants growth in controlled conditions (hydroponic, 16-h photoperiod, 70% humidity, 25/20 °C temperature), depended on its concentration in the substrate.
31177026	2	77	theme	25/20 °C	317:324	arg1	photoperiod					290:300	hydroponic, 16-h photoperiod	273:300	hydroponic, 16-h photoperiod	273:300	The effects of auxin IBA (indole-3-butyric acid) during 10-days on maize plants growth in controlled conditions (hydroponic, 16-h photoperiod, 70% humidity, 25/20 °C temperature), depended on its concentration in the substrate.
31177026	6	78	theme	cell	902:905	arg1	walls					907:911	Plant cell walls	896:911	Plant cell walls as cell structures	896:930	Plant cell walls as cell structures ensure cell enlargement and plant growth, and have to react to auxin stimulus by modification of their components.
31177026	4	79	theme	low	604:606	arg1	concentration					608:620	A low concentration	602:620	A low concentration (10-11 M) of IBA	602:637	A low concentration (10-11 M) of IBA stimulated root growth but affected neither the development of apoplasmic barriers, nor the amount of lignin.
31177026	4	79	theme	low	604:606	arg1	M					629:629	10-11 M	623:629	10-11 M	623:629	A low concentration (10-11 M) of IBA stimulated root growth but affected neither the development of apoplasmic barriers, nor the amount of lignin.
31177026	0	80	theme	IBA	15:17	arg1	effects					4:10	The effects	0:10	The effects of IBA on the composition of maize	0:45	The effects of IBA on the composition of maize root cell walls.
31177026	5	81	theme	root	796:799	arg1	growth					801:806	the root growth	792:806	the root growth	792:806	Auxin in a 10-8 M concentration influenced the root growth to a minimal extent compare to the control, and it was the non-effective concentration.
31177026	10	82	theme	specific	1616:1623	arg1	stress					1633:1638	the specific tensile stress	1612:1638	the specific tensile stress of	1612:1641	This may be related to the deposition of polysaccharides and lignin in the cell wall, which help maintain the specific tensile stress of, and turgor pressure on, the cell walls.
31177026	3	83	theme	high	390:393	arg1	concentration					395:407	A high concentration	388:407	A high concentration (10-7 M) of IBA	388:423	A high concentration (10-7 M) of IBA inhibited root growth, evoked the development of apoplasmic barriers (Casparian bands and suberin lamellae) closer to the root apex, and elevated the amount of lignin in roots.
31177026	3	83	theme	high	390:393	arg1	M					415:415	10-7 M	410:415	10-7 M	410:415	A high concentration (10-7 M) of IBA inhibited root growth, evoked the development of apoplasmic barriers (Casparian bands and suberin lamellae) closer to the root apex, and elevated the amount of lignin in roots.
31177026	9	84	dep	3.3	1447:1449	arg1	to					1444:1445	to	1444:1445	to	1444:1445	The ratio of the non-hydrolysable part to the Klason lignin increased from 1.3 to 3.3 with increasing auxin concentrations in the substrate.
31177026	3	85	theme	closer	533:538	arg1	barriers					485:492	apoplasmic barriers	474:492	apoplasmic barriers (Casparian bands and suberin lamellae) closer to the root apex	474:555	A high concentration (10-7 M) of IBA inhibited root growth, evoked the development of apoplasmic barriers (Casparian bands and suberin lamellae) closer to the root apex, and elevated the amount of lignin in roots.
31177026	8	86	theme	auxin	1187:1191	arg1	presence					1175:1182	The presence	1171:1182	The presence of auxin in the substrate	1171:1208	The presence of auxin in the substrate affected all three components of this fraction - Klason lignin and both the by acid (2 M TFA) non-hydrolysable and the hydrolysable parts of this complex.
31177026	9	87	theme	non-hydrolysable	1382:1397	arg1	part					1399:1402	the non-hydrolysable part	1378:1402	the non-hydrolysable part	1378:1402	The ratio of the non-hydrolysable part to the Klason lignin increased from 1.3 to 3.3 with increasing auxin concentrations in the substrate.
31177026	7	88	theme	significant	1065:1075	arg1	changes					1077:1083	the most significant changes	1056:1083	the most significant changes in the composition of the PF III fraction (lignocellulosic complex) of the cell wall	1056:1168	We found the most significant changes in the composition of the PF III fraction (lignocellulosic complex) of the cell wall.
31177026	2	89	theme	70	303:304	arg1	%					305:305	%	305:305	%	305:305	The effects of auxin IBA (indole-3-butyric acid) during 10-days on maize plants growth in controlled conditions (hydroponic, 16-h photoperiod, 70% humidity, 25/20 °C temperature), depended on its concentration in the substrate.
31244795	8	0	theme	response	1444:1451	arg1	methodology					1461:1471	response surface methodology	1444:1471	response surface methodology (RSM)	1444:1477	The main factors affecting shell and straw degradation were determined to be reaction time and temperature; and both factors were optimized by central composite design (CCD) of response surface methodology (RSM) to enhance the efficiency of degradation.
31244795	8	0	theme	response	1444:1451	arg1	RSM					1474:1476	RSM	1474:1476	RSM	1474:1476	The main factors affecting shell and straw degradation were determined to be reaction time and temperature; and both factors were optimized by central composite design (CCD) of response surface methodology (RSM) to enhance the efficiency of degradation.
31244795	3	1	theme	Tk-ChiA	512:518	arg1	gene					520:523	The Tk-ChiA gene	508:523	The Tk-ChiA gene	508:523	The Tk-ChiA gene was cloned and expressed on Pichia pastoris GS115.
31244795	9	2	theme	promising	1608:1616	arg1	hydrolase					1618:1626	a promising hydrolase	1606:1626	a promising hydrolase for shell and straw waste treatment, conversion, and utilization	1606:1691	Our findings suggest that Tk-ChiA with dual thermostable hydrolytic activities maybe a promising hydrolase for shell and straw waste treatment, conversion, and utilization.
31244795	7	3	dep	h.	1085:1086	arg1	capable					1113:1119	capable	1113:1119	capable	1113:1119	It showed thermal stability on incubation for 4 h at temperatures ranging from 70 to 80°C and remained more than 40% of its maximum activity after pre-incubation at 100°C for 4 h. Particularly, Tk-ChiA is capable of degrading shrimp shell and rice straw through scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FT-IR) analysis.
31244795	4	4	theme	molecular	580:588	arg1	weight					590:595	The molecular weight	576:595	The molecular weight of the purified Tk-ChiA	576:619	The molecular weight of the purified Tk-ChiA is about 130.0 kDa.
31244795	4	4	theme	molecular	580:588	arg1	kDa					636:638	about 130.0 kDa	624:638	about 130.0 kDa	624:638	The molecular weight of the purified Tk-ChiA is about 130.0 kDa.
31244795	7	5	theme	scanning	1170:1177	arg1	SEM					1200:1202	SEM	1200:1202	SEM	1200:1202	It showed thermal stability on incubation for 4 h at temperatures ranging from 70 to 80°C and remained more than 40% of its maximum activity after pre-incubation at 100°C for 4 h. Particularly, Tk-ChiA is capable of degrading shrimp shell and rice straw through scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FT-IR) analysis.
31244795	7	5	theme	scanning	1170:1177	arg1	microscopy					1188:1197	scanning electron microscopy	1170:1197	scanning electron microscopy (SEM)	1170:1203	It showed thermal stability on incubation for 4 h at temperatures ranging from 70 to 80°C and remained more than 40% of its maximum activity after pre-incubation at 100°C for 4 h. Particularly, Tk-ChiA is capable of degrading shrimp shell and rice straw through scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FT-IR) analysis.
31244795	8	6	theme	methodology	1461:1471	arg1	CCD					1436:1438	CCD	1436:1438	CCD	1436:1438	The main factors affecting shell and straw degradation were determined to be reaction time and temperature; and both factors were optimized by central composite design (CCD) of response surface methodology (RSM) to enhance the efficiency of degradation.
31244795	8	6	theme	methodology	1461:1471	arg1	design					1428:1433	central composite design	1410:1433	central composite design (CCD) of response surface methodology (RSM) to enhance the efficiency of degradation	1410:1518	The main factors affecting shell and straw degradation were determined to be reaction time and temperature; and both factors were optimized by central composite design (CCD) of response surface methodology (RSM) to enhance the efficiency of degradation.
31244795	0	7	dep	Shell	114:118	arg1	Degradation					130:140	Degradation	130:140	Degradation	130:140	An Archaeal Chitinase With a Secondary Capacity for Catalyzing Cellulose and Its Biotechnological Applications in Shell and Straw Degradation.
31244795	1	8	theme	attractive	277:286	arg1	resource					288:295	an attractive resource	274:295	an attractive resource for gene cloning	274:312	Numerous thermostable enzymes have been reported from the hyperthermophilic archaeon Thermococcus kodakarensis KOD1, which made it an attractive resource for gene cloning.
31244795	0	9	theme	Biotechnological	81:96	arg1	Applications					98:109	Its Biotechnological Applications	77:109	Its Biotechnological Applications in Shell and Straw Degradation	77:140	An Archaeal Chitinase With a Secondary Capacity for Catalyzing Cellulose and Its Biotechnological Applications in Shell and Straw Degradation.
31244795	6	10	theme	Purified	812:819	arg1	Tk-ChiA					821:827	Purified Tk-ChiA	812:827	Purified Tk-ChiA	812:827	Purified Tk-ChiA showed maximal activity for hydrolyzing CMC-Na at temperature 65°C and pH 7.0.
31244795	3	11	theme	Pichia	553:558	arg1	pastoris					560:567	Pichia pastoris GS115	553:573	Pichia pastoris GS115	553:573	The Tk-ChiA gene was cloned and expressed on Pichia pastoris GS115.
31244795	2	12	dep	reported	329:336	arg1	form					369:372	form	369:372	reported a glycosyl hydrolase (Tk-ChiA) form T. Kodakarensis with dual hydrolytic activity due to the presence of three binding domains with affinity toward chitin and cellulose	329:505	This research reported a glycosyl hydrolase (Tk-ChiA) form T. Kodakarensis with dual hydrolytic activity due to the presence of three binding domains with affinity toward chitin and cellulose.
31244795	9	13	with	Tk-ChiA	1547:1553	arg1	activities					1589:1598	dual thermostable hydrolytic activities	1560:1598	dual thermostable hydrolytic activities	1560:1598	Our findings suggest that Tk-ChiA with dual thermostable hydrolytic activities maybe a promising hydrolase for shell and straw waste treatment, conversion, and utilization.
31244795	5	14	theme	dual	739:742	arg1	activity					755:762	dual hydrolysis activity	739:762	dual hydrolysis activity	739:762	By using chitosan, CMC-Na and other polysaccharides as substrates, we confirmed that Tk-ChiA with dual hydrolysis activity preferably hydrolyzes both chitosan and CMC-Na.
31244795	7	15	theme	rice	1151:1154	arg1	straw					1156:1160	rice straw	1151:1160	rice straw	1151:1160	It showed thermal stability on incubation for 4 h at temperatures ranging from 70 to 80°C and remained more than 40% of its maximum activity after pre-incubation at 100°C for 4 h. Particularly, Tk-ChiA is capable of degrading shrimp shell and rice straw through scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FT-IR) analysis.
31244795	2	16	theme	hydrolytic	400:409	arg1	activity					411:418	dual hydrolytic activity	395:418	dual hydrolytic activity due to the presence of three binding domains with affinity toward chitin and cellulose	395:505	This research reported a glycosyl hydrolase (Tk-ChiA) form T. Kodakarensis with dual hydrolytic activity due to the presence of three binding domains with affinity toward chitin and cellulose.
31244795	2	17	theme	domains	457:463	arg1	presence					431:438	the presence	427:438	the presence of three binding domains with affinity toward chitin and cellulose	427:505	This research reported a glycosyl hydrolase (Tk-ChiA) form T. Kodakarensis with dual hydrolytic activity due to the presence of three binding domains with affinity toward chitin and cellulose.
31244795	2	18	theme	dual	395:398	arg1	activity					411:418	dual hydrolytic activity	395:418	dual hydrolytic activity due to the presence of three binding domains with affinity toward chitin and cellulose	395:505	This research reported a glycosyl hydrolase (Tk-ChiA) form T. Kodakarensis with dual hydrolytic activity due to the presence of three binding domains with affinity toward chitin and cellulose.
31244795	2	19	theme	binding	449:455	arg1	domains					457:463	three binding domains	443:463	three binding domains with affinity toward chitin and cellulose	443:505	This research reported a glycosyl hydrolase (Tk-ChiA) form T. Kodakarensis with dual hydrolytic activity due to the presence of three binding domains with affinity toward chitin and cellulose.
31244795	7	20	from	temperatures	961:972	arg1	incubation					939:948	incubation	939:948	incubation for 4 h at temperatures ranging from 70 to 80°C	939:996	It showed thermal stability on incubation for 4 h at temperatures ranging from 70 to 80°C and remained more than 40% of its maximum activity after pre-incubation at 100°C for 4 h. Particularly, Tk-ChiA is capable of degrading shrimp shell and rice straw through scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FT-IR) analysis.
31244795	6	21	theme	maximal	836:842	arg1	activity					844:851	maximal activity	836:851	maximal activity for hydrolyzing CMC-Na at temperature 65°C and pH 7.0	836:905	Purified Tk-ChiA showed maximal activity for hydrolyzing CMC-Na at temperature 65°C and pH 7.0.
31244795	7	22	theme	shrimp	1134:1139	arg1	shell					1141:1145	shrimp shell	1134:1145	shrimp shell	1134:1145	It showed thermal stability on incubation for 4 h at temperatures ranging from 70 to 80°C and remained more than 40% of its maximum activity after pre-incubation at 100°C for 4 h. Particularly, Tk-ChiA is capable of degrading shrimp shell and rice straw through scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FT-IR) analysis.
31244795	1	23	theme	gene	301:304	arg1	cloning					306:312	gene cloning	301:312	gene cloning	301:312	Numerous thermostable enzymes have been reported from the hyperthermophilic archaeon Thermococcus kodakarensis KOD1, which made it an attractive resource for gene cloning.
31244795	8	24	theme	composite	1418:1426	arg1	CCD					1436:1438	CCD	1436:1438	CCD	1436:1438	The main factors affecting shell and straw degradation were determined to be reaction time and temperature; and both factors were optimized by central composite design (CCD) of response surface methodology (RSM) to enhance the efficiency of degradation.
31244795	8	24	theme	composite	1418:1426	arg1	design					1428:1433	central composite design	1410:1433	central composite design (CCD) of response surface methodology (RSM) to enhance the efficiency of degradation	1410:1518	The main factors affecting shell and straw degradation were determined to be reaction time and temperature; and both factors were optimized by central composite design (CCD) of response surface methodology (RSM) to enhance the efficiency of degradation.
31244795	5	25	with	Tk-ChiA	726:732	arg1	activity					755:762	dual hydrolysis activity	739:762	dual hydrolysis activity	739:762	By using chitosan, CMC-Na and other polysaccharides as substrates, we confirmed that Tk-ChiA with dual hydrolysis activity preferably hydrolyzes both chitosan and CMC-Na.
31244795	7	26	dep	transform	1217:1225	arg1	infrared					1227:1234	infrared	1227:1234	transform infrared spectroscopy (FT-IR) analysis	1217:1264	It showed thermal stability on incubation for 4 h at temperatures ranging from 70 to 80°C and remained more than 40% of its maximum activity after pre-incubation at 100°C for 4 h. Particularly, Tk-ChiA is capable of degrading shrimp shell and rice straw through scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FT-IR) analysis.
31244795	1	27	theme	hyperthermophilic	201:217	arg1	archaeon					219:226	the hyperthermophilic archaeon	197:226	the hyperthermophilic archaeon Thermococcus kodakarensis KOD1	197:257	Numerous thermostable enzymes have been reported from the hyperthermophilic archaeon Thermococcus kodakarensis KOD1, which made it an attractive resource for gene cloning.
31244795	8	28	theme	degradation	1508:1518	arg1	efficiency					1494:1503	the efficiency	1490:1503	the efficiency of degradation	1490:1518	The main factors affecting shell and straw degradation were determined to be reaction time and temperature; and both factors were optimized by central composite design (CCD) of response surface methodology (RSM) to enhance the efficiency of degradation.
31244795	8	29	theme	central	1410:1416	arg1	CCD					1436:1438	CCD	1436:1438	CCD	1436:1438	The main factors affecting shell and straw degradation were determined to be reaction time and temperature; and both factors were optimized by central composite design (CCD) of response surface methodology (RSM) to enhance the efficiency of degradation.
31244795	8	29	theme	central	1410:1416	arg1	design					1428:1433	central composite design	1410:1433	central composite design (CCD) of response surface methodology (RSM) to enhance the efficiency of degradation	1410:1518	The main factors affecting shell and straw degradation were determined to be reaction time and temperature; and both factors were optimized by central composite design (CCD) of response surface methodology (RSM) to enhance the efficiency of degradation.
31244795	8	30	theme	reaction	1344:1351	arg1	factors					1276:1282	The main factors	1267:1282	The main factors affecting shell and straw degradation	1267:1320	The main factors affecting shell and straw degradation were determined to be reaction time and temperature; and both factors were optimized by central composite design (CCD) of response surface methodology (RSM) to enhance the efficiency of degradation.
31244795	8	30	theme	reaction	1344:1351	arg1	time					1353:1356	reaction time	1344:1356	reaction time	1344:1356	The main factors affecting shell and straw degradation were determined to be reaction time and temperature; and both factors were optimized by central composite design (CCD) of response surface methodology (RSM) to enhance the efficiency of degradation.
31244795	0	31	theme	Secondary	29:37	arg1	Capacity					39:46	a Secondary Capacity	27:46	a Secondary Capacity for Catalyzing Cellulose and Its Biotechnological Applications in Shell and Straw Degradation	27:140	An Archaeal Chitinase With a Secondary Capacity for Catalyzing Cellulose and Its Biotechnological Applications in Shell and Straw Degradation.
31244795	7	32	theme	maximum	1032:1038	arg1	activity					1040:1047	its maximum activity	1028:1047	its maximum activity	1028:1047	It showed thermal stability on incubation for 4 h at temperatures ranging from 70 to 80°C and remained more than 40% of its maximum activity after pre-incubation at 100°C for 4 h. Particularly, Tk-ChiA is capable of degrading shrimp shell and rice straw through scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FT-IR) analysis.
31244795	9	33	theme	waste	1648:1652	arg1	treatment					1654:1662	waste treatment	1648:1662	waste treatment	1648:1662	Our findings suggest that Tk-ChiA with dual thermostable hydrolytic activities maybe a promising hydrolase for shell and straw waste treatment, conversion, and utilization.
31244795	7	34	dep	80°C	993:996	arg1	to					990:991	to	990:991	to	990:991	It showed thermal stability on incubation for 4 h at temperatures ranging from 70 to 80°C and remained more than 40% of its maximum activity after pre-incubation at 100°C for 4 h. Particularly, Tk-ChiA is capable of degrading shrimp shell and rice straw through scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FT-IR) analysis.
31244795	1	35	theme	Thermococcus	228:239	arg1	KOD1					254:257	Thermococcus kodakarensis KOD1	228:257	the hyperthermophilic archaeon Thermococcus kodakarensis KOD1	197:257	Numerous thermostable enzymes have been reported from the hyperthermophilic archaeon Thermococcus kodakarensis KOD1, which made it an attractive resource for gene cloning.
31244795	7	36	theme	activity	1040:1047	arg1	activity					1040:1047	its maximum activity	1028:1047	its maximum activity	1028:1047	It showed thermal stability on incubation for 4 h at temperatures ranging from 70 to 80°C and remained more than 40% of its maximum activity after pre-incubation at 100°C for 4 h. Particularly, Tk-ChiA is capable of degrading shrimp shell and rice straw through scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FT-IR) analysis.
31244795	7	36	theme	activity	1040:1047	arg1	%					1023:1023	more than 40%	1011:1023	more than 40% of its maximum activity	1011:1047	It showed thermal stability on incubation for 4 h at temperatures ranging from 70 to 80°C and remained more than 40% of its maximum activity after pre-incubation at 100°C for 4 h. Particularly, Tk-ChiA is capable of degrading shrimp shell and rice straw through scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FT-IR) analysis.
31244795	0	37	from	Cellulose	63:71	arg1	Straw					124:128	Straw	124:128	Straw	124:128	An Archaeal Chitinase With a Secondary Capacity for Catalyzing Cellulose and Its Biotechnological Applications in Shell and Straw Degradation.
31244795	0	37	from	Cellulose	63:71	arg1	Shell					114:118	Shell	114:118	Shell	114:118	An Archaeal Chitinase With a Secondary Capacity for Catalyzing Cellulose and Its Biotechnological Applications in Shell and Straw Degradation.
31244795	8	38	dep	shell	1294:1298	arg1	degradation					1310:1320	degradation	1310:1320	degradation	1310:1320	The main factors affecting shell and straw degradation were determined to be reaction time and temperature; and both factors were optimized by central composite design (CCD) of response surface methodology (RSM) to enhance the efficiency of degradation.
31244795	2	39	theme	due	420:422	arg1	activity					411:418	dual hydrolytic activity	395:418	dual hydrolytic activity due to the presence of three binding domains with affinity toward chitin and cellulose	395:505	This research reported a glycosyl hydrolase (Tk-ChiA) form T. Kodakarensis with dual hydrolytic activity due to the presence of three binding domains with affinity toward chitin and cellulose.
31244795	9	40	dep	shell	1632:1636	arg1	treatment					1654:1662	waste treatment	1648:1662	waste treatment	1648:1662	Our findings suggest that Tk-ChiA with dual thermostable hydrolytic activities maybe a promising hydrolase for shell and straw waste treatment, conversion, and utilization.
31244795	7	41	from	100°C	1073:1077	arg1	pre-incubation					1055:1068	pre-incubation	1055:1068	pre-incubation at 100°C for 4 h. Particularly, Tk-ChiA is capable of degrading shrimp shell and rice straw through scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FT-IR) analysis	1055:1264	It showed thermal stability on incubation for 4 h at temperatures ranging from 70 to 80°C and remained more than 40% of its maximum activity after pre-incubation at 100°C for 4 h. Particularly, Tk-ChiA is capable of degrading shrimp shell and rice straw through scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FT-IR) analysis.
31244795	0	42	with	Chitinase	12:20	arg1	Capacity					39:46	a Secondary Capacity	27:46	a Secondary Capacity for Catalyzing Cellulose and Its Biotechnological Applications in Shell and Straw Degradation	27:140	An Archaeal Chitinase With a Secondary Capacity for Catalyzing Cellulose and Its Biotechnological Applications in Shell and Straw Degradation.
31244795	7	43	theme	FT-IR	1250:1254	arg1	analysis					1257:1264	spectroscopy (FT-IR) analysis	1236:1264	spectroscopy (FT-IR) analysis	1236:1264	It showed thermal stability on incubation for 4 h at temperatures ranging from 70 to 80°C and remained more than 40% of its maximum activity after pre-incubation at 100°C for 4 h. Particularly, Tk-ChiA is capable of degrading shrimp shell and rice straw through scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FT-IR) analysis.
31244795	5	44	theme	other	671:675	arg1	polysaccharides					677:691	other polysaccharides	671:691	other polysaccharides	671:691	By using chitosan, CMC-Na and other polysaccharides as substrates, we confirmed that Tk-ChiA with dual hydrolysis activity preferably hydrolyzes both chitosan and CMC-Na.
31244795	5	44	theme	other	671:675	arg1	chitosan					650:657	chitosan	650:657	chitosan	650:657	By using chitosan, CMC-Na and other polysaccharides as substrates, we confirmed that Tk-ChiA with dual hydrolysis activity preferably hydrolyzes both chitosan and CMC-Na.
31244795	5	44	theme	other	671:675	arg1	substrates					696:705	substrates	696:705	substrates	696:705	By using chitosan, CMC-Na and other polysaccharides as substrates, we confirmed that Tk-ChiA with dual hydrolysis activity preferably hydrolyzes both chitosan and CMC-Na.
31244795	5	44	theme	other	671:675	arg1	CMC-Na					660:665	CMC-Na	660:665	CMC-Na	660:665	By using chitosan, CMC-Na and other polysaccharides as substrates, we confirmed that Tk-ChiA with dual hydrolysis activity preferably hydrolyzes both chitosan and CMC-Na.
31244795	1	45	theme	thermostable	152:163	arg1	enzymes					165:171	Numerous thermostable enzymes	143:171	Numerous thermostable enzymes	143:171	Numerous thermostable enzymes have been reported from the hyperthermophilic archaeon Thermococcus kodakarensis KOD1, which made it an attractive resource for gene cloning.
31244795	2	46	with	domains	457:463	arg1	affinity					470:477	affinity	470:477	affinity toward chitin and cellulose	470:505	This research reported a glycosyl hydrolase (Tk-ChiA) form T. Kodakarensis with dual hydrolytic activity due to the presence of three binding domains with affinity toward chitin and cellulose.
31244795	7	47	theme	thermal	918:924	arg1	stability					926:934	thermal stability	918:934	thermal stability	918:934	It showed thermal stability on incubation for 4 h at temperatures ranging from 70 to 80°C and remained more than 40% of its maximum activity after pre-incubation at 100°C for 4 h. Particularly, Tk-ChiA is capable of degrading shrimp shell and rice straw through scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FT-IR) analysis.
31244795	9	48	theme	thermostable	1565:1576	arg1	activities					1589:1598	dual thermostable hydrolytic activities	1560:1598	dual thermostable hydrolytic activities	1560:1598	Our findings suggest that Tk-ChiA with dual thermostable hydrolytic activities maybe a promising hydrolase for shell and straw waste treatment, conversion, and utilization.
31244795	7	49	theme	spectroscopy	1236:1247	arg1	analysis					1257:1264	spectroscopy (FT-IR) analysis	1236:1264	spectroscopy (FT-IR) analysis	1236:1264	It showed thermal stability on incubation for 4 h at temperatures ranging from 70 to 80°C and remained more than 40% of its maximum activity after pre-incubation at 100°C for 4 h. Particularly, Tk-ChiA is capable of degrading shrimp shell and rice straw through scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FT-IR) analysis.
31244795	4	50	theme	Tk-ChiA	613:619	arg1	weight					590:595	The molecular weight	576:595	The molecular weight of the purified Tk-ChiA	576:619	The molecular weight of the purified Tk-ChiA is about 130.0 kDa.
31244795	4	50	theme	Tk-ChiA	613:619	arg1	kDa					636:638	about 130.0 kDa	624:638	about 130.0 kDa	624:638	The molecular weight of the purified Tk-ChiA is about 130.0 kDa.
31244795	0	51	from	Applications	98:109	arg1	Straw					124:128	Straw	124:128	Straw	124:128	An Archaeal Chitinase With a Secondary Capacity for Catalyzing Cellulose and Its Biotechnological Applications in Shell and Straw Degradation.
31244795	0	51	from	Applications	98:109	arg1	Shell					114:118	Shell	114:118	Shell	114:118	An Archaeal Chitinase With a Secondary Capacity for Catalyzing Cellulose and Its Biotechnological Applications in Shell and Straw Degradation.
31244795	9	52	theme	hydrolytic	1578:1587	arg1	activities					1589:1598	dual thermostable hydrolytic activities	1560:1598	dual thermostable hydrolytic activities	1560:1598	Our findings suggest that Tk-ChiA with dual thermostable hydrolytic activities maybe a promising hydrolase for shell and straw waste treatment, conversion, and utilization.
31244795	2	53	theme	glycosyl	340:347	arg1	Tk-ChiA					360:366	Tk-ChiA	360:366	Tk-ChiA	360:366	This research reported a glycosyl hydrolase (Tk-ChiA) form T. Kodakarensis with dual hydrolytic activity due to the presence of three binding domains with affinity toward chitin and cellulose.
31244795	2	53	theme	glycosyl	340:347	arg1	hydrolase					349:357	a glycosyl hydrolase	338:357	a glycosyl hydrolase (Tk-ChiA)	338:367	This research reported a glycosyl hydrolase (Tk-ChiA) form T. Kodakarensis with dual hydrolytic activity due to the presence of three binding domains with affinity toward chitin and cellulose.
31244795	4	54	theme	purified	604:611	arg1	Tk-ChiA					613:619	the purified Tk-ChiA	600:619	the purified Tk-ChiA	600:619	The molecular weight of the purified Tk-ChiA is about 130.0 kDa.
31244795	5	55	theme	hydrolysis	744:753	arg1	activity					755:762	dual hydrolysis activity	739:762	dual hydrolysis activity	739:762	By using chitosan, CMC-Na and other polysaccharides as substrates, we confirmed that Tk-ChiA with dual hydrolysis activity preferably hydrolyzes both chitosan and CMC-Na.
31244795	1	56	theme	kodakarensis	241:252	arg1	KOD1					254:257	Thermococcus kodakarensis KOD1	228:257	the hyperthermophilic archaeon Thermococcus kodakarensis KOD1	197:257	Numerous thermostable enzymes have been reported from the hyperthermophilic archaeon Thermococcus kodakarensis KOD1, which made it an attractive resource for gene cloning.
31244795	1	57	theme	Numerous	143:150	arg1	enzymes					165:171	Numerous thermostable enzymes	143:171	Numerous thermostable enzymes	143:171	Numerous thermostable enzymes have been reported from the hyperthermophilic archaeon Thermococcus kodakarensis KOD1, which made it an attractive resource for gene cloning.
31244795	8	58	theme	main	1271:1274	arg1	factors					1276:1282	The main factors	1267:1282	The main factors affecting shell and straw degradation	1267:1320	The main factors affecting shell and straw degradation were determined to be reaction time and temperature; and both factors were optimized by central composite design (CCD) of response surface methodology (RSM) to enhance the efficiency of degradation.
31244795	8	58	theme	main	1271:1274	arg1	time					1353:1356	reaction time	1344:1356	reaction time	1344:1356	The main factors affecting shell and straw degradation were determined to be reaction time and temperature; and both factors were optimized by central composite design (CCD) of response surface methodology (RSM) to enhance the efficiency of degradation.
31244795	8	59	theme	surface	1453:1459	arg1	methodology					1461:1471	response surface methodology	1444:1471	response surface methodology (RSM)	1444:1477	The main factors affecting shell and straw degradation were determined to be reaction time and temperature; and both factors were optimized by central composite design (CCD) of response surface methodology (RSM) to enhance the efficiency of degradation.
31244795	8	59	theme	surface	1453:1459	arg1	RSM					1474:1476	RSM	1474:1476	RSM	1474:1476	The main factors affecting shell and straw degradation were determined to be reaction time and temperature; and both factors were optimized by central composite design (CCD) of response surface methodology (RSM) to enhance the efficiency of degradation.
31244795	1	60	dep	archaeon	219:226	arg1	KOD1					254:257	Thermococcus kodakarensis KOD1	228:257	the hyperthermophilic archaeon Thermococcus kodakarensis KOD1	197:257	Numerous thermostable enzymes have been reported from the hyperthermophilic archaeon Thermococcus kodakarensis KOD1, which made it an attractive resource for gene cloning.
31244795	0	61	theme	Archaeal	3:10	arg1	Chitinase					12:20	An Archaeal Chitinase	0:20	An Archaeal Chitinase With a Secondary Capacity for Catalyzing Cellulose and Its Biotechnological Applications in Shell and Straw Degradation.	0:141	An Archaeal Chitinase With a Secondary Capacity for Catalyzing Cellulose and Its Biotechnological Applications in Shell and Straw Degradation.
31244795	9	62	theme	dual	1560:1563	arg1	activities					1589:1598	dual thermostable hydrolytic activities	1560:1598	dual thermostable hydrolytic activities	1560:1598	Our findings suggest that Tk-ChiA with dual thermostable hydrolytic activities maybe a promising hydrolase for shell and straw waste treatment, conversion, and utilization.
31244795	7	63	theme	electron	1179:1186	arg1	SEM					1200:1202	SEM	1200:1202	SEM	1200:1202	It showed thermal stability on incubation for 4 h at temperatures ranging from 70 to 80°C and remained more than 40% of its maximum activity after pre-incubation at 100°C for 4 h. Particularly, Tk-ChiA is capable of degrading shrimp shell and rice straw through scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FT-IR) analysis.
31244795	7	63	theme	electron	1179:1186	arg1	microscopy					1188:1197	scanning electron microscopy	1170:1197	scanning electron microscopy (SEM)	1170:1203	It showed thermal stability on incubation for 4 h at temperatures ranging from 70 to 80°C and remained more than 40% of its maximum activity after pre-incubation at 100°C for 4 h. Particularly, Tk-ChiA is capable of degrading shrimp shell and rice straw through scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FT-IR) analysis.
29434668	3	0	theme	tissue	536:541	arg1	quality					519:525	the overall quality	507:525	the overall quality of plant tissue	507:541	Four primary components found in the plant cell wall contribute to the overall quality of plant tissue and conversion characteristics, cellulose and hemicellulose polysaccharides are the primary targets for fuel conversion, while lignin and ash provide structure and defense.
29434668	2	1	theme	feedstock	309:317	arg1	crops					319:323	biofuel feedstock crops	301:323	biofuel feedstock crops	301:323	Improvement efforts in biofuel feedstock crops have been primarily focused on increasing biomass yield with less consideration for tissue quality or composition.
29434668	7	2	from	biomass	1279:1285	arg1	hallii					1293:1298	P. hallii	1290:1298	P. hallii	1290:1298	RESULTS Ash, lignin, glucan, and xylan comprise 68% of total dry biomass in P. hallii: comparable to other feedstocks.
29434668	1	3	attach	derived	167:173	arg2	component					228:236	an important component	215:236	an important component of current renewable energy strategies	215:275	BACKGROUND Biofuels derived from lignocellulosic plant material are an important component of current renewable energy strategies.
29434668	1	3	attach	derived	167:173	arg2	Biofuels					158:165	BACKGROUND Biofuels	147:165	BACKGROUND Biofuels derived from lignocellulosic plant material	147:209	BACKGROUND Biofuels derived from lignocellulosic plant material are an important component of current renewable energy strategies.
29434668	1	3	attach	derived	167:173	arg1	material					202:209	lignocellulosic plant material	180:209	lignocellulosic plant material	180:209	BACKGROUND Biofuels derived from lignocellulosic plant material are an important component of current renewable energy strategies.
29434668	10	4	theme	tissue	1720:1725	arg1	composition					1727:1737	leaf tissue composition	1715:1737	leaf tissue composition	1715:1737	We used high throughput phenotyping to map genomic regions that impact natural variation in leaf tissue composition.
29434668	5	5	from	ecotypes	1031:1038	arg1	switchgrass					1043:1053	switchgrass	1043:1053	switchgrass	1043:1053	Diversity in the mapping population was generated by crossing xeric and mesic varietals, comparative to northern upland and southern lowland ecotypes in switchgrass.
29434668	1	6	theme	lignocellulosic	180:194	arg1	material					202:209	lignocellulosic plant material	180:209	lignocellulosic plant material	180:209	BACKGROUND Biofuels derived from lignocellulosic plant material are an important component of current renewable energy strategies.
29434668	6	7	theme	wall	1197:1200	arg1	components					1202:1211	cell wall components	1192:1211	cell wall components	1192:1211	We use near-infrared spectroscopy with a primary analytical method to create a P. hallii specific calibration model to quickly quantify cell wall components.
29434668	11	8	theme	tissue	1782:1787	arg1	traits					1789:1794	tissue traits	1782:1794	tissue traits	1782:1794	Understanding the genetic architecture of tissue traits in a tractable model grass system will lead to a better understanding of cell wall structure as well as provide genomic resources for bioenergy crop breeding programs.
29434668	0	9	theme	perennial	115:123	arg1	hallii					139:144	the model C4 perennial grass Panicum hallii	102:144	the model C4 perennial grass Panicum hallii	102:144	Quantitative trait loci for cell wall composition traits measured using near-infrared spectroscopy in the model C4 perennial grass Panicum hallii.
29434668	6	10	theme	P.	1135:1136	arg1	model					1166:1170	a P. hallii specific calibration model	1133:1170	a P. hallii specific calibration model to quickly quantify cell wall components	1133:1211	We use near-infrared spectroscopy with a primary analytical method to create a P. hallii specific calibration model to quickly quantify cell wall components.
29434668	11	11	theme	structure	1879:1887	arg1	understanding					1852:1864	a better understanding	1843:1864	a better understanding of cell wall structure	1843:1887	Understanding the genetic architecture of tissue traits in a tractable model grass system will lead to a better understanding of cell wall structure as well as provide genomic resources for bioenergy crop breeding programs.
29434668	11	12	from	architecture	1766:1777	arg1	system					1823:1828	a tractable model grass system	1799:1828	a tractable model grass system	1799:1828	Understanding the genetic architecture of tissue traits in a tractable model grass system will lead to a better understanding of cell wall structure as well as provide genomic resources for bioenergy crop breeding programs.
29434668	11	13	theme	cell	1869:1872	arg1	structure					1879:1887	cell wall structure	1869:1887	cell wall structure	1869:1887	Understanding the genetic architecture of tissue traits in a tractable model grass system will lead to a better understanding of cell wall structure as well as provide genomic resources for bioenergy crop breeding programs.
29434668	11	14	theme	grass	1817:1821	arg1	system					1823:1828	a tractable model grass system	1799:1828	a tractable model grass system	1799:1828	Understanding the genetic architecture of tissue traits in a tractable model grass system will lead to a better understanding of cell wall structure as well as provide genomic resources for bioenergy crop breeding programs.
29434668	0	15	theme	Panicum	131:137	arg1	hallii					139:144	the model C4 perennial grass Panicum hallii	102:144	the model C4 perennial grass Panicum hallii	102:144	Quantitative trait loci for cell wall composition traits measured using near-infrared spectroscopy in the model C4 perennial grass Panicum hallii.
29434668	3	16	theme	fuel	647:650	arg1	conversion					652:661	fuel conversion	647:661	fuel conversion	647:661	Four primary components found in the plant cell wall contribute to the overall quality of plant tissue and conversion characteristics, cellulose and hemicellulose polysaccharides are the primary targets for fuel conversion, while lignin and ash provide structure and defense.
29434668	11	17	theme	model	1811:1815	arg1	system					1823:1828	a tractable model grass system	1799:1828	a tractable model grass system	1799:1828	Understanding the genetic architecture of tissue traits in a tractable model grass system will lead to a better understanding of cell wall structure as well as provide genomic resources for bioenergy crop breeding programs.
29434668	6	18	with	spectroscopy	1077:1088	arg1	method					1116:1121	a primary analytical method	1095:1121	a primary analytical method	1095:1121	We use near-infrared spectroscopy with a primary analytical method to create a P. hallii specific calibration model to quickly quantify cell wall components.
29434668	4	19	theme	tissue	755:760	arg1	characteristics					762:776	tissue characteristics	755:776	tissue characteristics	755:776	We explore the genetic architecture of tissue characteristics using a quantitative trait loci (QTL) mapping approach in Panicum hallii, a model lignocellulosic grass system.
29434668	11	20	theme	tractable	1801:1809	arg1	system					1823:1828	a tractable model grass system	1799:1828	a tractable model grass system	1799:1828	Understanding the genetic architecture of tissue traits in a tractable model grass system will lead to a better understanding of cell wall structure as well as provide genomic resources for bioenergy crop breeding programs.
29434668	11	21	theme	bioenergy	1930:1938	arg1	programs					1954:1961	bioenergy crop breeding programs	1930:1961	bioenergy crop breeding programs	1930:1961	Understanding the genetic architecture of tissue traits in a tractable model grass system will lead to a better understanding of cell wall structure as well as provide genomic resources for bioenergy crop breeding programs.
29434668	2	22	theme	Improvement	278:288	arg1	efforts					290:296	Improvement efforts	278:296	Improvement efforts in biofuel feedstock crops	278:323	Improvement efforts in biofuel feedstock crops have been primarily focused on increasing biomass yield with less consideration for tissue quality or composition.
29434668	9	23	theme	Panicum	1503:1509	arg1	model					1540:1544	the genomic model	1528:1544	the genomic model for its close relative and emerging biofuel crop	1528:1593	CONCLUSIONS Panicum hallii serves as the genomic model for its close relative and emerging biofuel crop, switchgrass (P. virgatum).
29434668	9	23	theme	Panicum	1503:1509	arg1	hallii					1511:1516	CONCLUSIONS Panicum hallii	1491:1516	CONCLUSIONS Panicum hallii	1491:1516	CONCLUSIONS Panicum hallii serves as the genomic model for its close relative and emerging biofuel crop, switchgrass (P. virgatum).
29434668	6	24	theme	near-infrared	1063:1075	arg1	spectroscopy					1077:1088	near-infrared spectroscopy	1063:1088	near-infrared spectroscopy with a primary analytical method	1063:1121	We use near-infrared spectroscopy with a primary analytical method to create a P. hallii specific calibration model to quickly quantify cell wall components.
29434668	8	25	theme	single	1469:1474	arg1	group					1484:1488	a single linkage group	1467:1488	a single linkage group	1467:1488	We identified 14 QTL and one epistatic interaction across these four cell wall traits and found almost half of the QTL to localize to a single linkage group.
29434668	7	26	theme	other	1315:1319	arg1	feedstocks					1321:1330	other feedstocks	1315:1330	other feedstocks	1315:1330	RESULTS Ash, lignin, glucan, and xylan comprise 68% of total dry biomass in P. hallii: comparable to other feedstocks.
29434668	1	27	theme	BACKGROUND	147:156	arg1	Biofuels					158:165	BACKGROUND Biofuels	147:165	BACKGROUND Biofuels derived from lignocellulosic plant material	147:209	BACKGROUND Biofuels derived from lignocellulosic plant material are an important component of current renewable energy strategies.
29434668	1	27	theme	BACKGROUND	147:156	arg1	component					228:236	an important component	215:236	an important component of current renewable energy strategies	215:275	BACKGROUND Biofuels derived from lignocellulosic plant material are an important component of current renewable energy strategies.
29434668	7	28	dep	RESULTS	1214:1220	arg1	Ash					1222:1224	Ash	1222:1224	Ash	1222:1224	RESULTS Ash, lignin, glucan, and xylan comprise 68% of total dry biomass in P. hallii: comparable to other feedstocks.
29434668	7	28	dep	RESULTS	1214:1220	arg1	RESULTS					1214:1220	RESULTS	1214:1220	RESULTS Ash, lignin, glucan, and xylan	1214:1251	RESULTS Ash, lignin, glucan, and xylan comprise 68% of total dry biomass in P. hallii: comparable to other feedstocks.
29434668	7	28	dep	RESULTS	1214:1220	arg1	glucan					1235:1240	glucan	1235:1240	glucan	1235:1240	RESULTS Ash, lignin, glucan, and xylan comprise 68% of total dry biomass in P. hallii: comparable to other feedstocks.
29434668	7	28	dep	RESULTS	1214:1220	arg1	xylan					1247:1251	xylan	1247:1251	xylan	1247:1251	RESULTS Ash, lignin, glucan, and xylan comprise 68% of total dry biomass in P. hallii: comparable to other feedstocks.
29434668	7	28	dep	RESULTS	1214:1220	arg1	lignin					1227:1232	lignin	1227:1232	lignin	1227:1232	RESULTS Ash, lignin, glucan, and xylan comprise 68% of total dry biomass in P. hallii: comparable to other feedstocks.
29434668	0	29	theme	near-infrared	72:84	arg1	spectroscopy					86:97	near-infrared spectroscopy	72:97	near-infrared spectroscopy	72:97	Quantitative trait loci for cell wall composition traits measured using near-infrared spectroscopy in the model C4 perennial grass Panicum hallii.
29434668	10	30	theme	natural	1694:1700	arg1	variation					1702:1710	natural variation	1694:1710	natural variation in leaf tissue composition	1694:1737	We used high throughput phenotyping to map genomic regions that impact natural variation in leaf tissue composition.
29434668	9	31	theme	emerging	1573:1580	arg1	crop					1590:1593	emerging biofuel crop	1573:1593	emerging biofuel crop	1573:1593	CONCLUSIONS Panicum hallii serves as the genomic model for its close relative and emerging biofuel crop, switchgrass (P. virgatum).
29434668	8	32	theme	epistatic	1362:1370	arg1	interaction					1372:1382	one epistatic interaction	1358:1382	one epistatic interaction	1358:1382	We identified 14 QTL and one epistatic interaction across these four cell wall traits and found almost half of the QTL to localize to a single linkage group.
29434668	10	33	theme	high	1631:1634	arg1	phenotyping					1647:1657	high throughput phenotyping	1631:1657	high throughput phenotyping	1631:1657	We used high throughput phenotyping to map genomic regions that impact natural variation in leaf tissue composition.
29434668	0	34	theme	model	106:110	arg1	hallii					139:144	the model C4 perennial grass Panicum hallii	102:144	the model C4 perennial grass Panicum hallii	102:144	Quantitative trait loci for cell wall composition traits measured using near-infrared spectroscopy in the model C4 perennial grass Panicum hallii.
29434668	5	35	theme	lowland	1023:1029	arg1	ecotypes					1031:1038	northern upland and southern lowland ecotypes	994:1038	northern upland and southern lowland ecotypes in switchgrass	994:1053	Diversity in the mapping population was generated by crossing xeric and mesic varietals, comparative to northern upland and southern lowland ecotypes in switchgrass.
29434668	4	36	theme	quantitative	786:797	arg1	QTL					811:813	QTL	811:813	QTL	811:813	We explore the genetic architecture of tissue characteristics using a quantitative trait loci (QTL) mapping approach in Panicum hallii, a model lignocellulosic grass system.
29434668	4	36	theme	quantitative	786:797	arg1	loci					805:808	quantitative trait loci	786:808	a quantitative trait loci (QTL) mapping approach	784:831	We explore the genetic architecture of tissue characteristics using a quantitative trait loci (QTL) mapping approach in Panicum hallii, a model lignocellulosic grass system.
29434668	11	37	theme	wall	1874:1877	arg1	structure					1879:1887	cell wall structure	1869:1887	cell wall structure	1869:1887	Understanding the genetic architecture of tissue traits in a tractable model grass system will lead to a better understanding of cell wall structure as well as provide genomic resources for bioenergy crop breeding programs.
29434668	0	38	theme	Quantitative	0:11	arg1	loci					19:22	Quantitative trait loci	0:22	Quantitative trait loci for cell wall composition traits	0:55	Quantitative trait loci for cell wall composition traits measured using near-infrared spectroscopy in the model C4 perennial grass Panicum hallii.
29434668	7	39	from	%	1264:1264	arg1	hallii					1293:1298	P. hallii	1290:1298	P. hallii	1290:1298	RESULTS Ash, lignin, glucan, and xylan comprise 68% of total dry biomass in P. hallii: comparable to other feedstocks.
29434668	5	40	theme	comparative	979:989	arg1	varietals					968:976	xeric and mesic varietals	952:976	xeric and mesic varietals	952:976	Diversity in the mapping population was generated by crossing xeric and mesic varietals, comparative to northern upland and southern lowland ecotypes in switchgrass.
29434668	4	41	theme	loci	805:808	arg1	approach					824:831	a quantitative trait loci (QTL) mapping approach	784:831	a quantitative trait loci (QTL) mapping approach	784:831	We explore the genetic architecture of tissue characteristics using a quantitative trait loci (QTL) mapping approach in Panicum hallii, a model lignocellulosic grass system.
29434668	11	42	theme	genomic	1908:1914	arg1	resources					1916:1924	genomic resources	1908:1924	genomic resources for bioenergy crop breeding programs	1908:1961	Understanding the genetic architecture of tissue traits in a tractable model grass system will lead to a better understanding of cell wall structure as well as provide genomic resources for bioenergy crop breeding programs.
29434668	5	43	theme	northern	994:1001	arg1	ecotypes					1031:1038	northern upland and southern lowland ecotypes	994:1038	northern upland and southern lowland ecotypes in switchgrass	994:1053	Diversity in the mapping population was generated by crossing xeric and mesic varietals, comparative to northern upland and southern lowland ecotypes in switchgrass.
29434668	2	44	from	efforts	290:296	arg1	crops					319:323	biofuel feedstock crops	301:323	biofuel feedstock crops	301:323	Improvement efforts in biofuel feedstock crops have been primarily focused on increasing biomass yield with less consideration for tissue quality or composition.
29434668	6	45	theme	primary	1097:1103	arg1	method					1116:1121	a primary analytical method	1095:1121	a primary analytical method	1095:1121	We use near-infrared spectroscopy with a primary analytical method to create a P. hallii specific calibration model to quickly quantify cell wall components.
29434668	3	46	theme	primary	445:451	arg1	components					453:462	Four primary components	440:462	Four primary components found in the plant cell wall	440:491	Four primary components found in the plant cell wall contribute to the overall quality of plant tissue and conversion characteristics, cellulose and hemicellulose polysaccharides are the primary targets for fuel conversion, while lignin and ash provide structure and defense.
29434668	8	47	theme	wall	1407:1410	arg1	traits					1412:1417	these four cell wall traits	1391:1417	these four cell wall traits	1391:1417	We identified 14 QTL and one epistatic interaction across these four cell wall traits and found almost half of the QTL to localize to a single linkage group.
29434668	3	48	theme	cell	483:486	arg1	wall					488:491	the plant cell wall	473:491	the plant cell wall	473:491	Four primary components found in the plant cell wall contribute to the overall quality of plant tissue and conversion characteristics, cellulose and hemicellulose polysaccharides are the primary targets for fuel conversion, while lignin and ash provide structure and defense.
29434668	0	49	theme	cell	28:31	arg1	traits					50:55	cell wall composition traits	28:55	cell wall composition traits	28:55	Quantitative trait loci for cell wall composition traits measured using near-infrared spectroscopy in the model C4 perennial grass Panicum hallii.
29434668	4	50	theme	lignocellulosic	860:874	arg1	system					882:887	a model lignocellulosic grass system	852:887	a model lignocellulosic grass system	852:887	We explore the genetic architecture of tissue characteristics using a quantitative trait loci (QTL) mapping approach in Panicum hallii, a model lignocellulosic grass system.
29434668	0	51	theme	composition	38:48	arg1	traits					50:55	cell wall composition traits	28:55	cell wall composition traits	28:55	Quantitative trait loci for cell wall composition traits measured using near-infrared spectroscopy in the model C4 perennial grass Panicum hallii.
29434668	11	52	theme	better	1845:1850	arg1	understanding					1852:1864	a better understanding	1843:1864	a better understanding of cell wall structure	1843:1887	Understanding the genetic architecture of tissue traits in a tractable model grass system will lead to a better understanding of cell wall structure as well as provide genomic resources for bioenergy crop breeding programs.
29434668	6	53	dep	P.	1135:1136	arg1	hallii					1138:1143	hallii	1138:1143	hallii	1138:1143	We use near-infrared spectroscopy with a primary analytical method to create a P. hallii specific calibration model to quickly quantify cell wall components.
29434668	10	54	theme	leaf	1715:1718	arg1	composition					1727:1737	leaf tissue composition	1715:1737	leaf tissue composition	1715:1737	We used high throughput phenotyping to map genomic regions that impact natural variation in leaf tissue composition.
29434668	1	55	theme	renewable	249:257	arg1	strategies					266:275	current renewable energy strategies	241:275	current renewable energy strategies	241:275	BACKGROUND Biofuels derived from lignocellulosic plant material are an important component of current renewable energy strategies.
29434668	6	56	theme	specific	1145:1152	arg1	model					1166:1170	a P. hallii specific calibration model	1133:1170	a P. hallii specific calibration model to quickly quantify cell wall components	1133:1211	We use near-infrared spectroscopy with a primary analytical method to create a P. hallii specific calibration model to quickly quantify cell wall components.
29434668	3	57	theme	plant	530:534	arg1	tissue					536:541	plant tissue	530:541	plant tissue	530:541	Four primary components found in the plant cell wall contribute to the overall quality of plant tissue and conversion characteristics, cellulose and hemicellulose polysaccharides are the primary targets for fuel conversion, while lignin and ash provide structure and defense.
29434668	1	58	theme	strategies	266:275	arg1	Biofuels					158:165	BACKGROUND Biofuels	147:165	BACKGROUND Biofuels derived from lignocellulosic plant material	147:209	BACKGROUND Biofuels derived from lignocellulosic plant material are an important component of current renewable energy strategies.
29434668	1	58	theme	strategies	266:275	arg1	component					228:236	an important component	215:236	an important component of current renewable energy strategies	215:275	BACKGROUND Biofuels derived from lignocellulosic plant material are an important component of current renewable energy strategies.
29434668	7	59	theme	total	1269:1273	arg1	biomass					1279:1285	total dry biomass	1269:1285	total dry biomass in P. hallii	1269:1298	RESULTS Ash, lignin, glucan, and xylan comprise 68% of total dry biomass in P. hallii: comparable to other feedstocks.
29434668	2	60	theme	biofuel	301:307	arg1	crops					319:323	biofuel feedstock crops	301:323	biofuel feedstock crops	301:323	Improvement efforts in biofuel feedstock crops have been primarily focused on increasing biomass yield with less consideration for tissue quality or composition.
29434668	1	61	theme	plant	196:200	arg1	material					202:209	lignocellulosic plant material	180:209	lignocellulosic plant material	180:209	BACKGROUND Biofuels derived from lignocellulosic plant material are an important component of current renewable energy strategies.
29434668	4	62	theme	grass	876:880	arg1	system					882:887	a model lignocellulosic grass system	852:887	a model lignocellulosic grass system	852:887	We explore the genetic architecture of tissue characteristics using a quantitative trait loci (QTL) mapping approach in Panicum hallii, a model lignocellulosic grass system.
29434668	10	63	from	variation	1702:1710	arg1	composition					1727:1737	leaf tissue composition	1715:1737	leaf tissue composition	1715:1737	We used high throughput phenotyping to map genomic regions that impact natural variation in leaf tissue composition.
29434668	9	64	dep	switchgrass	1596:1606	arg1	virgatum					1612:1619	P. virgatum	1609:1619	P. virgatum	1609:1619	CONCLUSIONS Panicum hallii serves as the genomic model for its close relative and emerging biofuel crop, switchgrass (P. virgatum).
29434668	5	65	theme	mapping	907:913	arg1	population					915:924	the mapping population	903:924	the mapping population	903:924	Diversity in the mapping population was generated by crossing xeric and mesic varietals, comparative to northern upland and southern lowland ecotypes in switchgrass.
29434668	7	66	from	hallii	1293:1298	arg1	biomass					1279:1285	total dry biomass	1269:1285	total dry biomass in P. hallii	1269:1298	RESULTS Ash, lignin, glucan, and xylan comprise 68% of total dry biomass in P. hallii: comparable to other feedstocks.
29434668	7	66	from	hallii	1293:1298	arg1	%					1264:1264	68%	1262:1264	68% of total dry biomass in P. hallii: comparable to other feedstocks	1262:1330	RESULTS Ash, lignin, glucan, and xylan comprise 68% of total dry biomass in P. hallii: comparable to other feedstocks.
29434668	0	67	theme	grass	125:129	arg1	hallii					139:144	the model C4 perennial grass Panicum hallii	102:144	the model C4 perennial grass Panicum hallii	102:144	Quantitative trait loci for cell wall composition traits measured using near-infrared spectroscopy in the model C4 perennial grass Panicum hallii.
29434668	3	68	theme	hemicellulose	589:601	arg1	cellulose					575:583	cellulose	575:583	cellulose	575:583	Four primary components found in the plant cell wall contribute to the overall quality of plant tissue and conversion characteristics, cellulose and hemicellulose polysaccharides are the primary targets for fuel conversion, while lignin and ash provide structure and defense.
29434668	3	68	theme	hemicellulose	589:601	arg1	polysaccharides					603:617	hemicellulose polysaccharides	589:617	hemicellulose polysaccharides	589:617	Four primary components found in the plant cell wall contribute to the overall quality of plant tissue and conversion characteristics, cellulose and hemicellulose polysaccharides are the primary targets for fuel conversion, while lignin and ash provide structure and defense.
29434668	3	68	theme	hemicellulose	589:601	arg1	characteristics					558:572	conversion characteristics	547:572	conversion characteristics	547:572	Four primary components found in the plant cell wall contribute to the overall quality of plant tissue and conversion characteristics, cellulose and hemicellulose polysaccharides are the primary targets for fuel conversion, while lignin and ash provide structure and defense.
29434668	3	68	theme	hemicellulose	589:601	arg1	targets					635:641	the primary targets	623:641	the primary targets for fuel conversion	623:661	Four primary components found in the plant cell wall contribute to the overall quality of plant tissue and conversion characteristics, cellulose and hemicellulose polysaccharides are the primary targets for fuel conversion, while lignin and ash provide structure and defense.
29434668	8	69	theme	QTL	1448:1450	arg1	half					1436:1439	almost half	1429:1439	almost half of the QTL	1429:1450	We identified 14 QTL and one epistatic interaction across these four cell wall traits and found almost half of the QTL to localize to a single linkage group.
29434668	6	70	theme	cell	1192:1195	arg1	components					1202:1211	cell wall components	1192:1211	cell wall components	1192:1211	We use near-infrared spectroscopy with a primary analytical method to create a P. hallii specific calibration model to quickly quantify cell wall components.
29434668	11	71	theme	traits	1789:1794	arg1	architecture					1766:1777	the genetic architecture	1754:1777	the genetic architecture of tissue traits in a tractable model grass system	1754:1828	Understanding the genetic architecture of tissue traits in a tractable model grass system will lead to a better understanding of cell wall structure as well as provide genomic resources for bioenergy crop breeding programs.
29434668	11	72	theme	crop	1940:1943	arg1	programs					1954:1961	bioenergy crop breeding programs	1930:1961	bioenergy crop breeding programs	1930:1961	Understanding the genetic architecture of tissue traits in a tractable model grass system will lead to a better understanding of cell wall structure as well as provide genomic resources for bioenergy crop breeding programs.
29434668	1	73	theme	important	218:226	arg1	Biofuels					158:165	BACKGROUND Biofuels	147:165	BACKGROUND Biofuels derived from lignocellulosic plant material	147:209	BACKGROUND Biofuels derived from lignocellulosic plant material are an important component of current renewable energy strategies.
29434668	1	73	theme	important	218:226	arg1	component					228:236	an important component	215:236	an important component of current renewable energy strategies	215:275	BACKGROUND Biofuels derived from lignocellulosic plant material are an important component of current renewable energy strategies.
29434668	3	74	theme	primary	627:633	arg1	cellulose					575:583	cellulose	575:583	cellulose	575:583	Four primary components found in the plant cell wall contribute to the overall quality of plant tissue and conversion characteristics, cellulose and hemicellulose polysaccharides are the primary targets for fuel conversion, while lignin and ash provide structure and defense.
29434668	3	74	theme	primary	627:633	arg1	polysaccharides					603:617	hemicellulose polysaccharides	589:617	hemicellulose polysaccharides	589:617	Four primary components found in the plant cell wall contribute to the overall quality of plant tissue and conversion characteristics, cellulose and hemicellulose polysaccharides are the primary targets for fuel conversion, while lignin and ash provide structure and defense.
29434668	3	74	theme	primary	627:633	arg1	characteristics					558:572	conversion characteristics	547:572	conversion characteristics	547:572	Four primary components found in the plant cell wall contribute to the overall quality of plant tissue and conversion characteristics, cellulose and hemicellulose polysaccharides are the primary targets for fuel conversion, while lignin and ash provide structure and defense.
29434668	3	74	theme	primary	627:633	arg1	targets					635:641	the primary targets	623:641	the primary targets for fuel conversion	623:661	Four primary components found in the plant cell wall contribute to the overall quality of plant tissue and conversion characteristics, cellulose and hemicellulose polysaccharides are the primary targets for fuel conversion, while lignin and ash provide structure and defense.
29434668	5	75	from	Diversity	890:898	arg1	population					915:924	the mapping population	903:924	the mapping population	903:924	Diversity in the mapping population was generated by crossing xeric and mesic varietals, comparative to northern upland and southern lowland ecotypes in switchgrass.
29434668	5	76	theme	xeric	952:956	arg1	varietals					968:976	xeric and mesic varietals	952:976	xeric and mesic varietals	952:976	Diversity in the mapping population was generated by crossing xeric and mesic varietals, comparative to northern upland and southern lowland ecotypes in switchgrass.
29434668	7	77	theme	biomass	1279:1285	arg1	biomass					1279:1285	total dry biomass	1269:1285	total dry biomass in P. hallii	1269:1298	RESULTS Ash, lignin, glucan, and xylan comprise 68% of total dry biomass in P. hallii: comparable to other feedstocks.
29434668	7	77	theme	biomass	1279:1285	arg1	%					1264:1264	68%	1262:1264	68% of total dry biomass in P. hallii: comparable to other feedstocks	1262:1330	RESULTS Ash, lignin, glucan, and xylan comprise 68% of total dry biomass in P. hallii: comparable to other feedstocks.
29434668	9	78	theme	CONCLUSIONS	1491:1501	arg1	model					1540:1544	the genomic model	1528:1544	the genomic model for its close relative and emerging biofuel crop	1528:1593	CONCLUSIONS Panicum hallii serves as the genomic model for its close relative and emerging biofuel crop, switchgrass (P. virgatum).
29434668	9	78	theme	CONCLUSIONS	1491:1501	arg1	hallii					1511:1516	CONCLUSIONS Panicum hallii	1491:1516	CONCLUSIONS Panicum hallii	1491:1516	CONCLUSIONS Panicum hallii serves as the genomic model for its close relative and emerging biofuel crop, switchgrass (P. virgatum).
29434668	8	79	theme	linkage	1476:1482	arg1	group					1484:1488	a single linkage group	1467:1488	a single linkage group	1467:1488	We identified 14 QTL and one epistatic interaction across these four cell wall traits and found almost half of the QTL to localize to a single linkage group.
29434668	9	80	theme	genomic	1532:1538	arg1	switchgrass					1596:1606	switchgrass	1596:1606	switchgrass (P. virgatum)	1596:1620	CONCLUSIONS Panicum hallii serves as the genomic model for its close relative and emerging biofuel crop, switchgrass (P. virgatum).
29434668	9	80	theme	genomic	1532:1538	arg1	model					1540:1544	the genomic model	1528:1544	the genomic model for its close relative and emerging biofuel crop	1528:1593	CONCLUSIONS Panicum hallii serves as the genomic model for its close relative and emerging biofuel crop, switchgrass (P. virgatum).
29434668	9	80	theme	genomic	1532:1538	arg1	hallii					1511:1516	CONCLUSIONS Panicum hallii	1491:1516	CONCLUSIONS Panicum hallii	1491:1516	CONCLUSIONS Panicum hallii serves as the genomic model for its close relative and emerging biofuel crop, switchgrass (P. virgatum).
29434668	4	81	theme	characteristics	762:776	arg1	architecture					739:750	the genetic architecture	727:750	the genetic architecture of tissue characteristics	727:776	We explore the genetic architecture of tissue characteristics using a quantitative trait loci (QTL) mapping approach in Panicum hallii, a model lignocellulosic grass system.
29434668	4	82	theme	genetic	731:737	arg1	architecture					739:750	the genetic architecture	727:750	the genetic architecture of tissue characteristics	727:776	We explore the genetic architecture of tissue characteristics using a quantitative trait loci (QTL) mapping approach in Panicum hallii, a model lignocellulosic grass system.
29434668	5	83	theme	upland	1003:1008	arg1	northern					994:1001	northern upland	994:1008	northern upland	994:1008	Diversity in the mapping population was generated by crossing xeric and mesic varietals, comparative to northern upland and southern lowland ecotypes in switchgrass.
29434668	10	84	theme	genomic	1666:1672	arg1	regions					1674:1680	genomic regions	1666:1680	genomic regions that impact natural variation in leaf tissue composition	1666:1737	We used high throughput phenotyping to map genomic regions that impact natural variation in leaf tissue composition.
29434668	0	85	theme	C4	112:113	arg1	hallii					139:144	the model C4 perennial grass Panicum hallii	102:144	the model C4 perennial grass Panicum hallii	102:144	Quantitative trait loci for cell wall composition traits measured using near-infrared spectroscopy in the model C4 perennial grass Panicum hallii.
29434668	5	86	theme	southern	1014:1021	arg1	ecotypes					1031:1038	northern upland and southern lowland ecotypes	994:1038	northern upland and southern lowland ecotypes in switchgrass	994:1053	Diversity in the mapping population was generated by crossing xeric and mesic varietals, comparative to northern upland and southern lowland ecotypes in switchgrass.
29434668	5	87	theme	mesic	962:966	arg1	varietals					968:976	xeric and mesic varietals	952:976	xeric and mesic varietals	952:976	Diversity in the mapping population was generated by crossing xeric and mesic varietals, comparative to northern upland and southern lowland ecotypes in switchgrass.
29434668	11	88	theme	genetic	1758:1764	arg1	architecture					1766:1777	the genetic architecture	1754:1777	the genetic architecture of tissue traits in a tractable model grass system	1754:1828	Understanding the genetic architecture of tissue traits in a tractable model grass system will lead to a better understanding of cell wall structure as well as provide genomic resources for bioenergy crop breeding programs.
29434668	3	89	theme	plant	477:481	arg1	wall					488:491	the plant cell wall	473:491	the plant cell wall	473:491	Four primary components found in the plant cell wall contribute to the overall quality of plant tissue and conversion characteristics, cellulose and hemicellulose polysaccharides are the primary targets for fuel conversion, while lignin and ash provide structure and defense.
29434668	0	90	theme	trait	13:17	arg1	loci					19:22	Quantitative trait loci	0:22	Quantitative trait loci for cell wall composition traits	0:55	Quantitative trait loci for cell wall composition traits measured using near-infrared spectroscopy in the model C4 perennial grass Panicum hallii.
29434668	9	91	theme	close	1554:1558	arg1	relative					1560:1567	its close relative	1550:1567	its close relative	1550:1567	CONCLUSIONS Panicum hallii serves as the genomic model for its close relative and emerging biofuel crop, switchgrass (P. virgatum).
29434668	2	92	theme	tissue	409:414	arg1	quality					416:422	tissue quality	409:422	tissue quality	409:422	Improvement efforts in biofuel feedstock crops have been primarily focused on increasing biomass yield with less consideration for tissue quality or composition.
29434668	3	93	located	found	464:468	arg2	components					453:462	Four primary components	440:462	Four primary components found in the plant cell wall	440:491	Four primary components found in the plant cell wall contribute to the overall quality of plant tissue and conversion characteristics, cellulose and hemicellulose polysaccharides are the primary targets for fuel conversion, while lignin and ash provide structure and defense.
29434668	3	93	located	found	464:468	arg1	wall					488:491	the plant cell wall	473:491	the plant cell wall	473:491	Four primary components found in the plant cell wall contribute to the overall quality of plant tissue and conversion characteristics, cellulose and hemicellulose polysaccharides are the primary targets for fuel conversion, while lignin and ash provide structure and defense.
29434668	10	94	used	used	1626:1629	arg2	We					1623:1624	We	1623:1624	We	1623:1624	We used high throughput phenotyping to map genomic regions that impact natural variation in leaf tissue composition.
29434668	6	95	theme	analytical	1105:1114	arg1	method					1116:1121	a primary analytical method	1095:1121	a primary analytical method	1095:1121	We use near-infrared spectroscopy with a primary analytical method to create a P. hallii specific calibration model to quickly quantify cell wall components.
29434668	4	96	theme	mapping	816:822	arg1	approach					824:831	a quantitative trait loci (QTL) mapping approach	784:831	a quantitative trait loci (QTL) mapping approach	784:831	We explore the genetic architecture of tissue characteristics using a quantitative trait loci (QTL) mapping approach in Panicum hallii, a model lignocellulosic grass system.
29434668	4	97	theme	trait	799:803	arg1	QTL					811:813	QTL	811:813	QTL	811:813	We explore the genetic architecture of tissue characteristics using a quantitative trait loci (QTL) mapping approach in Panicum hallii, a model lignocellulosic grass system.
29434668	4	97	theme	trait	799:803	arg1	loci					805:808	quantitative trait loci	786:808	a quantitative trait loci (QTL) mapping approach	784:831	We explore the genetic architecture of tissue characteristics using a quantitative trait loci (QTL) mapping approach in Panicum hallii, a model lignocellulosic grass system.
29434668	6	98	theme	calibration	1154:1164	arg1	model					1166:1170	a P. hallii specific calibration model	1133:1170	a P. hallii specific calibration model to quickly quantify cell wall components	1133:1211	We use near-infrared spectroscopy with a primary analytical method to create a P. hallii specific calibration model to quickly quantify cell wall components.
29434668	0	99	theme	wall	33:36	arg1	traits					50:55	cell wall composition traits	28:55	cell wall composition traits	28:55	Quantitative trait loci for cell wall composition traits measured using near-infrared spectroscopy in the model C4 perennial grass Panicum hallii.
29434668	9	100	theme	biofuel	1582:1588	arg1	crop					1590:1593	emerging biofuel crop	1573:1593	emerging biofuel crop	1573:1593	CONCLUSIONS Panicum hallii serves as the genomic model for its close relative and emerging biofuel crop, switchgrass (P. virgatum).
29434668	7	101	theme	comparable	1301:1310	arg1	biomass					1279:1285	total dry biomass	1269:1285	total dry biomass in P. hallii	1269:1298	RESULTS Ash, lignin, glucan, and xylan comprise 68% of total dry biomass in P. hallii: comparable to other feedstocks.
29434668	7	101	theme	comparable	1301:1310	arg1	%					1264:1264	68%	1262:1264	68% of total dry biomass in P. hallii: comparable to other feedstocks	1262:1330	RESULTS Ash, lignin, glucan, and xylan comprise 68% of total dry biomass in P. hallii: comparable to other feedstocks.
29434668	8	102	theme	cell	1402:1405	arg1	traits					1412:1417	these four cell wall traits	1391:1417	these four cell wall traits	1391:1417	We identified 14 QTL and one epistatic interaction across these four cell wall traits and found almost half of the QTL to localize to a single linkage group.
29434668	10	103	theme	throughput	1636:1645	arg1	phenotyping					1647:1657	high throughput phenotyping	1631:1657	high throughput phenotyping	1631:1657	We used high throughput phenotyping to map genomic regions that impact natural variation in leaf tissue composition.
29434668	4	104	theme	model	854:858	arg1	system					882:887	a model lignocellulosic grass system	852:887	a model lignocellulosic grass system	852:887	We explore the genetic architecture of tissue characteristics using a quantitative trait loci (QTL) mapping approach in Panicum hallii, a model lignocellulosic grass system.
29434668	2	105	theme	biomass	367:373	arg1	yield					375:379	biomass yield	367:379	biomass yield	367:379	Improvement efforts in biofuel feedstock crops have been primarily focused on increasing biomass yield with less consideration for tissue quality or composition.
29434668	11	106	theme	breeding	1945:1952	arg1	programs					1954:1961	bioenergy crop breeding programs	1930:1961	bioenergy crop breeding programs	1930:1961	Understanding the genetic architecture of tissue traits in a tractable model grass system will lead to a better understanding of cell wall structure as well as provide genomic resources for bioenergy crop breeding programs.
29434668	3	107	theme	overall	511:517	arg1	quality					519:525	the overall quality	507:525	the overall quality of plant tissue	507:541	Four primary components found in the plant cell wall contribute to the overall quality of plant tissue and conversion characteristics, cellulose and hemicellulose polysaccharides are the primary targets for fuel conversion, while lignin and ash provide structure and defense.
29434668	1	108	theme	current	241:247	arg1	strategies					266:275	current renewable energy strategies	241:275	current renewable energy strategies	241:275	BACKGROUND Biofuels derived from lignocellulosic plant material are an important component of current renewable energy strategies.
29434668	7	109	theme	dry	1275:1277	arg1	biomass					1279:1285	total dry biomass	1269:1285	total dry biomass in P. hallii	1269:1298	RESULTS Ash, lignin, glucan, and xylan comprise 68% of total dry biomass in P. hallii: comparable to other feedstocks.
29434668	3	110	theme	conversion	547:556	arg1	cellulose					575:583	cellulose	575:583	cellulose	575:583	Four primary components found in the plant cell wall contribute to the overall quality of plant tissue and conversion characteristics, cellulose and hemicellulose polysaccharides are the primary targets for fuel conversion, while lignin and ash provide structure and defense.
29434668	3	110	theme	conversion	547:556	arg1	polysaccharides					603:617	hemicellulose polysaccharides	589:617	hemicellulose polysaccharides	589:617	Four primary components found in the plant cell wall contribute to the overall quality of plant tissue and conversion characteristics, cellulose and hemicellulose polysaccharides are the primary targets for fuel conversion, while lignin and ash provide structure and defense.
29434668	3	110	theme	conversion	547:556	arg1	characteristics					558:572	conversion characteristics	547:572	conversion characteristics	547:572	Four primary components found in the plant cell wall contribute to the overall quality of plant tissue and conversion characteristics, cellulose and hemicellulose polysaccharides are the primary targets for fuel conversion, while lignin and ash provide structure and defense.
29434668	3	110	theme	conversion	547:556	arg1	targets					635:641	the primary targets	623:641	the primary targets for fuel conversion	623:661	Four primary components found in the plant cell wall contribute to the overall quality of plant tissue and conversion characteristics, cellulose and hemicellulose polysaccharides are the primary targets for fuel conversion, while lignin and ash provide structure and defense.
29434668	1	111	theme	energy	259:264	arg1	strategies					266:275	current renewable energy strategies	241:275	current renewable energy strategies	241:275	BACKGROUND Biofuels derived from lignocellulosic plant material are an important component of current renewable energy strategies.
29864141	7	0	theme	cellular	1162:1169	arg1	membranes					1171:1179	cellular membranes	1162:1179	cellular membranes	1162:1179	We observed that the Mar1 protein differentially localizes to cellular membranes in a condition dependent manner, and we have further shown that the mar1Δ mutant displays defects in intracellular trafficking, resulting in a mislocalization of the β-glucan synthase catalytic subunit, Fks1.
29864141	3	1	with	mutants	357:363	arg1	changes					370:376	changes	370:376	changes in their cell wall	370:395	In a genetic screen for mutants with changes in their cell wall, we identified a novel protein, Mar1, that controls cell wall organization and immune evasion.
29864141	4	2	theme	strain	541:546	arg1	studies					511:517	phenotypic studies	500:517	phenotypic studies of a loss-of-function strain	500:546	Through phenotypic studies of a loss-of-function strain, we have demonstrated that the mar1Δ mutant has an aberrant cell surface and a defect in polysaccharide capsule attachment, resulting in attenuated virulence.
29864141	2	3	theme	cell	255:258	arg1	remodeling					265:274	This cell wall remodeling	250:274	This cell wall remodeling	250:274	This cell wall remodeling is essential for host immune avoidance by this pathogen.
29864141	9	4	theme	macrophage	1632:1641	arg1	activation					1643:1652	the observed macrophage activation	1619:1652	the observed macrophage activation	1619:1652	We established that several host innate immune signaling proteins are required for the observed macrophage activation, including the Card9 and MyD88 adaptor proteins, as well as the Dectin-1 and TLR2 pattern recognition receptors.
29864141	6	5	theme	cell	1082:1085	arg1	layers					1092:1097	the outer cell wall layers	1072:1097	the outer cell wall layers	1072:1097	However, HPLC analysis of whole cell walls and RT-PCR analysis of cell wall synthase genes demonstrated that this increased chitin exposure is likely due to decreased levels of glucans and mannans in the outer cell wall layers.
29864141	4	6	theme	cell	608:611	arg1	surface					613:619	an aberrant cell surface	596:619	an aberrant cell surface	596:619	Through phenotypic studies of a loss-of-function strain, we have demonstrated that the mar1Δ mutant has an aberrant cell surface and a defect in polysaccharide capsule attachment, resulting in attenuated virulence.
29864141	1	7	theme	cell	185:188	arg1	wall					190:193	its cell wall	181:193	its cell wall	181:193	The human fungal pathogen, Cryptococcus neoformans, dramatically alters its cell wall, both in size and composition, upon entering the host.
29864141	6	8	theme	cell	904:907	arg1	walls					909:913	whole cell walls	898:913	whole cell walls	898:913	However, HPLC analysis of whole cell walls and RT-PCR analysis of cell wall synthase genes demonstrated that this increased chitin exposure is likely due to decreased levels of glucans and mannans in the outer cell wall layers.
29864141	5	9	theme	wall	782:785	arg1	chitin					787:792	exposed cell wall chitin	769:792	exposed cell wall chitin	769:792	Furthermore, the mar1Δ mutant displays increased staining for exposed cell wall chitin and chitosan when the cells are grown in host-like tissue culture conditions.
29864141	1	10	theme	human	113:117	arg1	pathogen					126:133	The human fungal pathogen	109:133	The human fungal pathogen	109:133	The human fungal pathogen, Cryptococcus neoformans, dramatically alters its cell wall, both in size and composition, upon entering the host.
29864141	1	10	theme	human	113:117	arg1	neoformans					149:158	Cryptococcus neoformans	136:158	Cryptococcus neoformans	136:158	The human fungal pathogen, Cryptococcus neoformans, dramatically alters its cell wall, both in size and composition, upon entering the host.
29864141	10	11	theme	adaptive	1925:1932	arg1	response					1934:1941	this adaptive response	1920:1941	this adaptive response	1920:1941	These studies explore novel mechanisms by which a microbial pathogen regulates its cell surface in response to the host, as well as how dysregulation of this adaptive response leads to defective immune avoidance.
29864141	6	12	theme	HPLC	881:884	arg1	analysis					886:893	HPLC analysis	881:893	HPLC analysis of whole cell walls	881:913	However, HPLC analysis of whole cell walls and RT-PCR analysis of cell wall synthase genes demonstrated that this increased chitin exposure is likely due to decreased levels of glucans and mannans in the outer cell wall layers.
29864141	7	13	theme	intracellular	1282:1294	arg1	trafficking					1296:1306	intracellular trafficking	1282:1306	intracellular trafficking	1282:1306	We observed that the Mar1 protein differentially localizes to cellular membranes in a condition dependent manner, and we have further shown that the mar1Δ mutant displays defects in intracellular trafficking, resulting in a mislocalization of the β-glucan synthase catalytic subunit, Fks1.
29864141	4	14	theme	polysaccharide	637:650	arg1	attachment					660:669	polysaccharide capsule attachment	637:669	polysaccharide capsule attachment	637:669	Through phenotypic studies of a loss-of-function strain, we have demonstrated that the mar1Δ mutant has an aberrant cell surface and a defect in polysaccharide capsule attachment, resulting in attenuated virulence.
29864141	5	15	theme	culture	852:858	arg1	conditions					860:869	host-like tissue culture conditions	835:869	host-like tissue culture conditions	835:869	Furthermore, the mar1Δ mutant displays increased staining for exposed cell wall chitin and chitosan when the cells are grown in host-like tissue culture conditions.
29864141	9	16	theme	adaptor	1685:1691	arg1	proteins					1693:1700	the Card9 and MyD88 adaptor proteins	1665:1700	proteins	1693:1700	We established that several host innate immune signaling proteins are required for the observed macrophage activation, including the Card9 and MyD88 adaptor proteins, as well as the Dectin-1 and TLR2 pattern recognition receptors.
29864141	10	17	theme	defective	1952:1960	arg1	avoidance					1969:1977	defective immune avoidance	1952:1977	defective immune avoidance	1952:1977	These studies explore novel mechanisms by which a microbial pathogen regulates its cell surface in response to the host, as well as how dysregulation of this adaptive response leads to defective immune avoidance.
29864141	9	18	dep	Dectin-1	1718:1725	arg1	receptors					1756:1764	pattern recognition receptors	1736:1764	pattern recognition receptors	1736:1764	We established that several host innate immune signaling proteins are required for the observed macrophage activation, including the Card9 and MyD88 adaptor proteins, as well as the Dectin-1 and TLR2 pattern recognition receptors.
29864141	9	18	dep	Dectin-1	1718:1725	arg1	the					1714:1716	the	1714:1716	the	1714:1716	We established that several host innate immune signaling proteins are required for the observed macrophage activation, including the Card9 and MyD88 adaptor proteins, as well as the Dectin-1 and TLR2 pattern recognition receptors.
29864141	7	19	theme	synthase	1356:1363	arg1	Fks1					1384:1387	Fks1	1384:1387	Fks1	1384:1387	We observed that the Mar1 protein differentially localizes to cellular membranes in a condition dependent manner, and we have further shown that the mar1Δ mutant displays defects in intracellular trafficking, resulting in a mislocalization of the β-glucan synthase catalytic subunit, Fks1.
29864141	7	19	theme	synthase	1356:1363	arg1	subunit					1375:1381	the β-glucan synthase catalytic subunit	1343:1381	the β-glucan synthase catalytic subunit	1343:1381	We observed that the Mar1 protein differentially localizes to cellular membranes in a condition dependent manner, and we have further shown that the mar1Δ mutant displays defects in intracellular trafficking, resulting in a mislocalization of the β-glucan synthase catalytic subunit, Fks1.
29864141	6	20	theme	synthase	948:955	arg1	genes					957:961	cell wall synthase genes	938:961	cell wall synthase genes	938:961	However, HPLC analysis of whole cell walls and RT-PCR analysis of cell wall synthase genes demonstrated that this increased chitin exposure is likely due to decreased levels of glucans and mannans in the outer cell wall layers.
29864141	8	21	theme	surface	1401:1407	arg1	changes					1409:1415	These cell surface changes	1390:1415	These cell surface changes	1390:1415	These cell surface changes influence the host-pathogen interaction, resulting in increased macrophage activation to microbial challenge in vitro.
29864141	0	22	theme	synthases	57:65	arg1	trafficking					25:35	intracellular trafficking	11:35	intracellular trafficking of fungal cell wall synthases	11:65	Defects in intracellular trafficking of fungal cell wall synthases lead to aberrant host immune recognition.
29864141	3	23	theme	immune	476:481	arg1	evasion					483:489	immune evasion	476:489	immune evasion	476:489	In a genetic screen for mutants with changes in their cell wall, we identified a novel protein, Mar1, that controls cell wall organization and immune evasion.
29864141	0	24	theme	intracellular	11:23	arg1	trafficking					25:35	intracellular trafficking	11:35	intracellular trafficking of fungal cell wall synthases	11:65	Defects in intracellular trafficking of fungal cell wall synthases lead to aberrant host immune recognition.
29864141	1	25	theme	Cryptococcus	136:147	arg1	pathogen					126:133	The human fungal pathogen	109:133	The human fungal pathogen	109:133	The human fungal pathogen, Cryptococcus neoformans, dramatically alters its cell wall, both in size and composition, upon entering the host.
29864141	1	25	theme	Cryptococcus	136:147	arg1	neoformans					149:158	Cryptococcus neoformans	136:158	Cryptococcus neoformans	136:158	The human fungal pathogen, Cryptococcus neoformans, dramatically alters its cell wall, both in size and composition, upon entering the host.
29864141	6	26	theme	cell	938:941	arg1	genes					957:961	cell wall synthase genes	938:961	cell wall synthase genes	938:961	However, HPLC analysis of whole cell walls and RT-PCR analysis of cell wall synthase genes demonstrated that this increased chitin exposure is likely due to decreased levels of glucans and mannans in the outer cell wall layers.
29864141	10	27	theme	response	1934:1941	arg1	dysregulation					1903:1915	dysregulation	1903:1915	dysregulation of this adaptive response	1903:1941	These studies explore novel mechanisms by which a microbial pathogen regulates its cell surface in response to the host, as well as how dysregulation of this adaptive response leads to defective immune avoidance.
29864141	3	28	from	changes	370:376	arg1	wall					392:395	their cell wall	381:395	their cell wall	381:395	In a genetic screen for mutants with changes in their cell wall, we identified a novel protein, Mar1, that controls cell wall organization and immune evasion.
29864141	5	29	theme	host-like	835:843	arg1	conditions					860:869	host-like tissue culture conditions	835:869	host-like tissue culture conditions	835:869	Furthermore, the mar1Δ mutant displays increased staining for exposed cell wall chitin and chitosan when the cells are grown in host-like tissue culture conditions.
29864141	8	30	theme	host-pathogen	1431:1443	arg1	interaction					1445:1455	the host-pathogen interaction	1427:1455	the host-pathogen interaction	1427:1455	These cell surface changes influence the host-pathogen interaction, resulting in increased macrophage activation to microbial challenge in vitro.
29864141	0	31	theme	host	84:87	arg1	recognition					96:106	aberrant host immune recognition	75:106	aberrant host immune recognition	75:106	Defects in intracellular trafficking of fungal cell wall synthases lead to aberrant host immune recognition.
29864141	7	32	from	defects	1271:1277	arg1	trafficking					1296:1306	intracellular trafficking	1282:1306	intracellular trafficking	1282:1306	We observed that the Mar1 protein differentially localizes to cellular membranes in a condition dependent manner, and we have further shown that the mar1Δ mutant displays defects in intracellular trafficking, resulting in a mislocalization of the β-glucan synthase catalytic subunit, Fks1.
29864141	7	33	theme	mar1Δ	1249:1253	arg1	mutant					1255:1260	the mar1Δ mutant	1245:1260	the mar1Δ mutant	1245:1260	We observed that the Mar1 protein differentially localizes to cellular membranes in a condition dependent manner, and we have further shown that the mar1Δ mutant displays defects in intracellular trafficking, resulting in a mislocalization of the β-glucan synthase catalytic subunit, Fks1.
29864141	9	34	theme	pattern	1736:1742	arg1	receptors					1756:1764	pattern recognition receptors	1736:1764	pattern recognition receptors	1736:1764	We established that several host innate immune signaling proteins are required for the observed macrophage activation, including the Card9 and MyD88 adaptor proteins, as well as the Dectin-1 and TLR2 pattern recognition receptors.
29864141	10	35	dep	host	1882:1885	arg1	response					1866:1873	response	1866:1873	response	1866:1873	These studies explore novel mechanisms by which a microbial pathogen regulates its cell surface in response to the host, as well as how dysregulation of this adaptive response leads to defective immune avoidance.
29864141	6	36	theme	chitin	996:1001	arg1	exposure					1003:1010	this increased chitin exposure	981:1010	this increased chitin exposure	981:1010	However, HPLC analysis of whole cell walls and RT-PCR analysis of cell wall synthase genes demonstrated that this increased chitin exposure is likely due to decreased levels of glucans and mannans in the outer cell wall layers.
29864141	8	37	theme	macrophage	1481:1490	arg1	activation					1492:1501	increased macrophage activation	1471:1501	increased macrophage activation to microbial challenge in vitro	1471:1533	These cell surface changes influence the host-pathogen interaction, resulting in increased macrophage activation to microbial challenge in vitro.
29864141	4	38	theme	attenuated	685:694	arg1	virulence					696:704	attenuated virulence	685:704	attenuated virulence	685:704	Through phenotypic studies of a loss-of-function strain, we have demonstrated that the mar1Δ mutant has an aberrant cell surface and a defect in polysaccharide capsule attachment, resulting in attenuated virulence.
29864141	5	39	theme	exposed	769:775	arg1	chitin					787:792	exposed cell wall chitin	769:792	exposed cell wall chitin	769:792	Furthermore, the mar1Δ mutant displays increased staining for exposed cell wall chitin and chitosan when the cells are grown in host-like tissue culture conditions.
29864141	4	40	theme	phenotypic	500:509	arg1	studies					511:517	phenotypic studies	500:517	phenotypic studies of a loss-of-function strain	500:546	Through phenotypic studies of a loss-of-function strain, we have demonstrated that the mar1Δ mutant has an aberrant cell surface and a defect in polysaccharide capsule attachment, resulting in attenuated virulence.
29864141	5	41	theme	mar1Δ	724:728	arg1	mutant					730:735	the mar1Δ mutant	720:735	the mar1Δ mutant	720:735	Furthermore, the mar1Δ mutant displays increased staining for exposed cell wall chitin and chitosan when the cells are grown in host-like tissue culture conditions.
29864141	0	42	theme	cell	47:50	arg1	synthases					57:65	fungal cell wall synthases	40:65	fungal cell wall synthases	40:65	Defects in intracellular trafficking of fungal cell wall synthases lead to aberrant host immune recognition.
29864141	9	43	theme	several	1556:1562	arg1	proteins					1593:1600	several host innate immune signaling proteins	1556:1600	several host innate immune signaling proteins	1556:1600	We established that several host innate immune signaling proteins are required for the observed macrophage activation, including the Card9 and MyD88 adaptor proteins, as well as the Dectin-1 and TLR2 pattern recognition receptors.
29864141	2	44	theme	immune	298:303	arg1	avoidance					305:313	host immune avoidance	293:313	host immune avoidance by this pathogen	293:330	This cell wall remodeling is essential for host immune avoidance by this pathogen.
29864141	7	45	theme	β-glucan	1347:1354	arg1	Fks1					1384:1387	Fks1	1384:1387	Fks1	1384:1387	We observed that the Mar1 protein differentially localizes to cellular membranes in a condition dependent manner, and we have further shown that the mar1Δ mutant displays defects in intracellular trafficking, resulting in a mislocalization of the β-glucan synthase catalytic subunit, Fks1.
29864141	7	45	theme	β-glucan	1347:1354	arg1	subunit					1375:1381	the β-glucan synthase catalytic subunit	1343:1381	the β-glucan synthase catalytic subunit	1343:1381	We observed that the Mar1 protein differentially localizes to cellular membranes in a condition dependent manner, and we have further shown that the mar1Δ mutant displays defects in intracellular trafficking, resulting in a mislocalization of the β-glucan synthase catalytic subunit, Fks1.
29864141	9	46	theme	innate	1569:1574	arg1	proteins					1593:1600	several host innate immune signaling proteins	1556:1600	several host innate immune signaling proteins	1556:1600	We established that several host innate immune signaling proteins are required for the observed macrophage activation, including the Card9 and MyD88 adaptor proteins, as well as the Dectin-1 and TLR2 pattern recognition receptors.
29864141	7	47	theme	dependent	1196:1204	arg1	manner					1206:1211	a condition dependent manner	1184:1211	a condition dependent manner	1184:1211	We observed that the Mar1 protein differentially localizes to cellular membranes in a condition dependent manner, and we have further shown that the mar1Δ mutant displays defects in intracellular trafficking, resulting in a mislocalization of the β-glucan synthase catalytic subunit, Fks1.
29864141	10	48	theme	microbial	1817:1825	arg1	pathogen					1827:1834	a microbial pathogen	1815:1834	a microbial pathogen	1815:1834	These studies explore novel mechanisms by which a microbial pathogen regulates its cell surface in response to the host, as well as how dysregulation of this adaptive response leads to defective immune avoidance.
29864141	3	49	theme	cell	449:452	arg1	organization					459:470	cell wall organization	449:470	cell wall organization	449:470	In a genetic screen for mutants with changes in their cell wall, we identified a novel protein, Mar1, that controls cell wall organization and immune evasion.
29864141	9	50	theme	signaling	1583:1591	arg1	proteins					1593:1600	several host innate immune signaling proteins	1556:1600	several host innate immune signaling proteins	1556:1600	We established that several host innate immune signaling proteins are required for the observed macrophage activation, including the Card9 and MyD88 adaptor proteins, as well as the Dectin-1 and TLR2 pattern recognition receptors.
29864141	6	51	theme	decreased	1029:1037	arg1	levels					1039:1044	decreased levels	1029:1044	decreased levels of glucans and mannans in the outer cell wall layers	1029:1097	However, HPLC analysis of whole cell walls and RT-PCR analysis of cell wall synthase genes demonstrated that this increased chitin exposure is likely due to decreased levels of glucans and mannans in the outer cell wall layers.
29864141	10	52	dep	regulates	1836:1844	arg1	leads					1943:1947	leads	1943:1947	leads to defective immune avoidance	1943:1977	These studies explore novel mechanisms by which a microbial pathogen regulates its cell surface in response to the host, as well as how dysregulation of this adaptive response leads to defective immune avoidance.
29864141	10	53	theme	cell	1850:1853	arg1	surface					1855:1861	its cell surface	1846:1861	its cell surface	1846:1861	These studies explore novel mechanisms by which a microbial pathogen regulates its cell surface in response to the host, as well as how dysregulation of this adaptive response leads to defective immune avoidance.
29864141	2	54	theme	wall	260:263	arg1	remodeling					265:274	This cell wall remodeling	250:274	This cell wall remodeling	250:274	This cell wall remodeling is essential for host immune avoidance by this pathogen.
29864141	4	55	from	surface	613:619	arg1	attachment					660:669	polysaccharide capsule attachment	637:669	polysaccharide capsule attachment	637:669	Through phenotypic studies of a loss-of-function strain, we have demonstrated that the mar1Δ mutant has an aberrant cell surface and a defect in polysaccharide capsule attachment, resulting in attenuated virulence.
29864141	7	56	theme	catalytic	1365:1373	arg1	Fks1					1384:1387	Fks1	1384:1387	Fks1	1384:1387	We observed that the Mar1 protein differentially localizes to cellular membranes in a condition dependent manner, and we have further shown that the mar1Δ mutant displays defects in intracellular trafficking, resulting in a mislocalization of the β-glucan synthase catalytic subunit, Fks1.
29864141	7	56	theme	catalytic	1365:1373	arg1	subunit					1375:1381	the β-glucan synthase catalytic subunit	1343:1381	the β-glucan synthase catalytic subunit	1343:1381	We observed that the Mar1 protein differentially localizes to cellular membranes in a condition dependent manner, and we have further shown that the mar1Δ mutant displays defects in intracellular trafficking, resulting in a mislocalization of the β-glucan synthase catalytic subunit, Fks1.
29864141	4	57	theme	loss-of-function	524:539	arg1	strain					541:546	a loss-of-function strain	522:546	a loss-of-function strain	522:546	Through phenotypic studies of a loss-of-function strain, we have demonstrated that the mar1Δ mutant has an aberrant cell surface and a defect in polysaccharide capsule attachment, resulting in attenuated virulence.
29864141	7	58	theme	subunit	1375:1381	arg1	mislocalization					1324:1338	a mislocalization	1322:1338	a mislocalization of the β-glucan synthase catalytic subunit, Fks1	1322:1387	We observed that the Mar1 protein differentially localizes to cellular membranes in a condition dependent manner, and we have further shown that the mar1Δ mutant displays defects in intracellular trafficking, resulting in a mislocalization of the β-glucan synthase catalytic subunit, Fks1.
29864141	9	59	theme	observed	1623:1630	arg1	activation					1643:1652	the observed macrophage activation	1619:1652	the observed macrophage activation	1619:1652	We established that several host innate immune signaling proteins are required for the observed macrophage activation, including the Card9 and MyD88 adaptor proteins, as well as the Dectin-1 and TLR2 pattern recognition receptors.
29864141	6	60	theme	wall	1087:1090	arg1	layers					1092:1097	the outer cell wall layers	1072:1097	the outer cell wall layers	1072:1097	However, HPLC analysis of whole cell walls and RT-PCR analysis of cell wall synthase genes demonstrated that this increased chitin exposure is likely due to decreased levels of glucans and mannans in the outer cell wall layers.
29864141	6	61	theme	walls	909:913	arg1	analysis					926:933	RT-PCR analysis	919:933	RT-PCR analysis of cell wall synthase genes	919:961	However, HPLC analysis of whole cell walls and RT-PCR analysis of cell wall synthase genes demonstrated that this increased chitin exposure is likely due to decreased levels of glucans and mannans in the outer cell wall layers.
29864141	6	61	theme	walls	909:913	arg1	analysis					886:893	HPLC analysis	881:893	HPLC analysis of whole cell walls	881:913	However, HPLC analysis of whole cell walls and RT-PCR analysis of cell wall synthase genes demonstrated that this increased chitin exposure is likely due to decreased levels of glucans and mannans in the outer cell wall layers.
29864141	6	62	theme	outer	1076:1080	arg1	layers					1092:1097	the outer cell wall layers	1072:1097	the outer cell wall layers	1072:1097	However, HPLC analysis of whole cell walls and RT-PCR analysis of cell wall synthase genes demonstrated that this increased chitin exposure is likely due to decreased levels of glucans and mannans in the outer cell wall layers.
29864141	4	63	theme	aberrant	599:606	arg1	surface					613:619	an aberrant cell surface	596:619	an aberrant cell surface	596:619	Through phenotypic studies of a loss-of-function strain, we have demonstrated that the mar1Δ mutant has an aberrant cell surface and a defect in polysaccharide capsule attachment, resulting in attenuated virulence.
29864141	5	64	theme	cell	777:780	arg1	chitin					787:792	exposed cell wall chitin	769:792	exposed cell wall chitin	769:792	Furthermore, the mar1Δ mutant displays increased staining for exposed cell wall chitin and chitosan when the cells are grown in host-like tissue culture conditions.
29864141	6	65	theme	whole	898:902	arg1	walls					909:913	whole cell walls	898:913	whole cell walls	898:913	However, HPLC analysis of whole cell walls and RT-PCR analysis of cell wall synthase genes demonstrated that this increased chitin exposure is likely due to decreased levels of glucans and mannans in the outer cell wall layers.
29864141	1	66	dep	size	204:207	arg1	both					196:199	both	196:199	both	196:199	The human fungal pathogen, Cryptococcus neoformans, dramatically alters its cell wall, both in size and composition, upon entering the host.
29864141	4	67	theme	mar1Δ	579:583	arg1	mutant					585:590	the mar1Δ mutant	575:590	the mar1Δ mutant	575:590	Through phenotypic studies of a loss-of-function strain, we have demonstrated that the mar1Δ mutant has an aberrant cell surface and a defect in polysaccharide capsule attachment, resulting in attenuated virulence.
29864141	5	68	theme	tissue	845:850	arg1	conditions					860:869	host-like tissue culture conditions	835:869	host-like tissue culture conditions	835:869	Furthermore, the mar1Δ mutant displays increased staining for exposed cell wall chitin and chitosan when the cells are grown in host-like tissue culture conditions.
29864141	0	69	from	Defects	0:6	arg1	trafficking					25:35	intracellular trafficking	11:35	intracellular trafficking of fungal cell wall synthases	11:65	Defects in intracellular trafficking of fungal cell wall synthases lead to aberrant host immune recognition.
29864141	9	70	theme	MyD88	1679:1683	arg1	proteins					1693:1700	the Card9 and MyD88 adaptor proteins	1665:1700	proteins	1693:1700	We established that several host innate immune signaling proteins are required for the observed macrophage activation, including the Card9 and MyD88 adaptor proteins, as well as the Dectin-1 and TLR2 pattern recognition receptors.
29864141	8	71	theme	cell	1396:1399	arg1	changes					1409:1415	These cell surface changes	1390:1415	These cell surface changes	1390:1415	These cell surface changes influence the host-pathogen interaction, resulting in increased macrophage activation to microbial challenge in vitro.
29864141	10	72	theme	novel	1789:1793	arg1	mechanisms					1795:1804	novel mechanisms	1789:1804	novel mechanisms by which a microbial pathogen regulates its cell surface in response to the host, as well as how dysregulation of this adaptive response leads to defective immune avoidance	1789:1977	These studies explore novel mechanisms by which a microbial pathogen regulates its cell surface in response to the host, as well as how dysregulation of this adaptive response leads to defective immune avoidance.
29864141	1	73	theme	fungal	119:124	arg1	pathogen					126:133	The human fungal pathogen	109:133	The human fungal pathogen	109:133	The human fungal pathogen, Cryptococcus neoformans, dramatically alters its cell wall, both in size and composition, upon entering the host.
29864141	1	73	theme	fungal	119:124	arg1	neoformans					149:158	Cryptococcus neoformans	136:158	Cryptococcus neoformans	136:158	The human fungal pathogen, Cryptococcus neoformans, dramatically alters its cell wall, both in size and composition, upon entering the host.
29864141	6	74	theme	genes	957:961	arg1	analysis					926:933	RT-PCR analysis	919:933	RT-PCR analysis of cell wall synthase genes	919:961	However, HPLC analysis of whole cell walls and RT-PCR analysis of cell wall synthase genes demonstrated that this increased chitin exposure is likely due to decreased levels of glucans and mannans in the outer cell wall layers.
29864141	6	74	theme	genes	957:961	arg1	analysis					886:893	HPLC analysis	881:893	HPLC analysis of whole cell walls	881:913	However, HPLC analysis of whole cell walls and RT-PCR analysis of cell wall synthase genes demonstrated that this increased chitin exposure is likely due to decreased levels of glucans and mannans in the outer cell wall layers.
29864141	4	75	theme	capsule	652:658	arg1	attachment					660:669	polysaccharide capsule attachment	637:669	polysaccharide capsule attachment	637:669	Through phenotypic studies of a loss-of-function strain, we have demonstrated that the mar1Δ mutant has an aberrant cell surface and a defect in polysaccharide capsule attachment, resulting in attenuated virulence.
29864141	6	76	theme	wall	943:946	arg1	genes					957:961	cell wall synthase genes	938:961	cell wall synthase genes	938:961	However, HPLC analysis of whole cell walls and RT-PCR analysis of cell wall synthase genes demonstrated that this increased chitin exposure is likely due to decreased levels of glucans and mannans in the outer cell wall layers.
29864141	7	77	theme	Mar1	1121:1124	arg1	protein					1126:1132	the Mar1 protein	1117:1132	the Mar1 protein	1117:1132	We observed that the Mar1 protein differentially localizes to cellular membranes in a condition dependent manner, and we have further shown that the mar1Δ mutant displays defects in intracellular trafficking, resulting in a mislocalization of the β-glucan synthase catalytic subunit, Fks1.
29864141	0	78	theme	aberrant	75:82	arg1	recognition					96:106	aberrant host immune recognition	75:106	aberrant host immune recognition	75:106	Defects in intracellular trafficking of fungal cell wall synthases lead to aberrant host immune recognition.
29864141	0	79	theme	immune	89:94	arg1	recognition					96:106	aberrant host immune recognition	75:106	aberrant host immune recognition	75:106	Defects in intracellular trafficking of fungal cell wall synthases lead to aberrant host immune recognition.
29864141	6	80	theme	RT-PCR	919:924	arg1	analysis					926:933	RT-PCR analysis	919:933	RT-PCR analysis of cell wall synthase genes	919:961	However, HPLC analysis of whole cell walls and RT-PCR analysis of cell wall synthase genes demonstrated that this increased chitin exposure is likely due to decreased levels of glucans and mannans in the outer cell wall layers.
29864141	10	81	theme	immune	1962:1967	arg1	avoidance					1969:1977	defective immune avoidance	1952:1977	defective immune avoidance	1952:1977	These studies explore novel mechanisms by which a microbial pathogen regulates its cell surface in response to the host, as well as how dysregulation of this adaptive response leads to defective immune avoidance.
29864141	8	82	theme	increased	1471:1479	arg1	activation					1492:1501	increased macrophage activation	1471:1501	increased macrophage activation to microbial challenge in vitro	1471:1533	These cell surface changes influence the host-pathogen interaction, resulting in increased macrophage activation to microbial challenge in vitro.
29864141	5	83	theme	increased	746:754	arg1	staining					756:763	increased staining	746:763	increased staining for exposed cell wall chitin and chitosan	746:805	Furthermore, the mar1Δ mutant displays increased staining for exposed cell wall chitin and chitosan when the cells are grown in host-like tissue culture conditions.
29864141	9	84	theme	recognition	1744:1754	arg1	receptors					1756:1764	pattern recognition receptors	1736:1764	pattern recognition receptors	1736:1764	We established that several host innate immune signaling proteins are required for the observed macrophage activation, including the Card9 and MyD88 adaptor proteins, as well as the Dectin-1 and TLR2 pattern recognition receptors.
29864141	3	85	theme	cell	387:390	arg1	wall					392:395	their cell wall	381:395	their cell wall	381:395	In a genetic screen for mutants with changes in their cell wall, we identified a novel protein, Mar1, that controls cell wall organization and immune evasion.
29864141	6	86	theme	increased	986:994	arg1	exposure					1003:1010	this increased chitin exposure	981:1010	this increased chitin exposure	981:1010	However, HPLC analysis of whole cell walls and RT-PCR analysis of cell wall synthase genes demonstrated that this increased chitin exposure is likely due to decreased levels of glucans and mannans in the outer cell wall layers.
29864141	3	87	theme	genetic	338:344	arg1	screen					346:351	a genetic screen	336:351	a genetic screen for mutants with changes in their cell wall	336:395	In a genetic screen for mutants with changes in their cell wall, we identified a novel protein, Mar1, that controls cell wall organization and immune evasion.
29864141	8	88	theme	microbial	1506:1514	arg1	challenge					1516:1524	microbial challenge	1506:1524	microbial challenge	1506:1524	These cell surface changes influence the host-pathogen interaction, resulting in increased macrophage activation to microbial challenge in vitro.
29864141	3	89	theme	novel	414:418	arg1	Mar1					429:432	Mar1	429:432	Mar1	429:432	In a genetic screen for mutants with changes in their cell wall, we identified a novel protein, Mar1, that controls cell wall organization and immune evasion.
29864141	3	89	theme	novel	414:418	arg1	protein					420:426	a novel protein	412:426	a novel protein	412:426	In a genetic screen for mutants with changes in their cell wall, we identified a novel protein, Mar1, that controls cell wall organization and immune evasion.
29864141	0	90	theme	fungal	40:45	arg1	synthases					57:65	fungal cell wall synthases	40:65	fungal cell wall synthases	40:65	Defects in intracellular trafficking of fungal cell wall synthases lead to aberrant host immune recognition.
29864141	0	91	theme	wall	52:55	arg1	synthases					57:65	fungal cell wall synthases	40:65	fungal cell wall synthases	40:65	Defects in intracellular trafficking of fungal cell wall synthases lead to aberrant host immune recognition.
29864141	6	92	theme	mannans	1061:1067	arg1	levels					1039:1044	decreased levels	1029:1044	decreased levels of glucans and mannans in the outer cell wall layers	1029:1097	However, HPLC analysis of whole cell walls and RT-PCR analysis of cell wall synthase genes demonstrated that this increased chitin exposure is likely due to decreased levels of glucans and mannans in the outer cell wall layers.
29864141	9	93	theme	host	1564:1567	arg1	proteins					1593:1600	several host innate immune signaling proteins	1556:1600	several host innate immune signaling proteins	1556:1600	We established that several host innate immune signaling proteins are required for the observed macrophage activation, including the Card9 and MyD88 adaptor proteins, as well as the Dectin-1 and TLR2 pattern recognition receptors.
29864141	6	94	theme	glucans	1049:1055	arg1	levels					1039:1044	decreased levels	1029:1044	decreased levels of glucans and mannans in the outer cell wall layers	1029:1097	However, HPLC analysis of whole cell walls and RT-PCR analysis of cell wall synthase genes demonstrated that this increased chitin exposure is likely due to decreased levels of glucans and mannans in the outer cell wall layers.
29864141	4	95	contain	has	592:594	arg2	surface					613:619	an aberrant cell surface	596:619	an aberrant cell surface	596:619	Through phenotypic studies of a loss-of-function strain, we have demonstrated that the mar1Δ mutant has an aberrant cell surface and a defect in polysaccharide capsule attachment, resulting in attenuated virulence.
29864141	4	95	contain	has	592:594	arg1	mutant					585:590	the mar1Δ mutant	575:590	the mar1Δ mutant	575:590	Through phenotypic studies of a loss-of-function strain, we have demonstrated that the mar1Δ mutant has an aberrant cell surface and a defect in polysaccharide capsule attachment, resulting in attenuated virulence.
29864141	4	95	contain	has	592:594	arg2	defect					627:632	a defect	625:632	a defect in polysaccharide capsule attachment	625:669	Through phenotypic studies of a loss-of-function strain, we have demonstrated that the mar1Δ mutant has an aberrant cell surface and a defect in polysaccharide capsule attachment, resulting in attenuated virulence.
29864141	2	96	theme	host	293:296	arg1	avoidance					305:313	host immune avoidance	293:313	host immune avoidance by this pathogen	293:330	This cell wall remodeling is essential for host immune avoidance by this pathogen.
29864141	9	97	theme	immune	1576:1581	arg1	proteins					1593:1600	several host innate immune signaling proteins	1556:1600	several host innate immune signaling proteins	1556:1600	We established that several host innate immune signaling proteins are required for the observed macrophage activation, including the Card9 and MyD88 adaptor proteins, as well as the Dectin-1 and TLR2 pattern recognition receptors.
29864141	6	98	from	levels	1039:1044	arg1	layers					1092:1097	the outer cell wall layers	1072:1097	the outer cell wall layers	1072:1097	However, HPLC analysis of whole cell walls and RT-PCR analysis of cell wall synthase genes demonstrated that this increased chitin exposure is likely due to decreased levels of glucans and mannans in the outer cell wall layers.
29864141	3	99	theme	wall	454:457	arg1	organization					459:470	cell wall organization	449:470	cell wall organization	449:470	In a genetic screen for mutants with changes in their cell wall, we identified a novel protein, Mar1, that controls cell wall organization and immune evasion.
29864141	4	100	from	defect	627:632	arg1	attachment					660:669	polysaccharide capsule attachment	637:669	polysaccharide capsule attachment	637:669	Through phenotypic studies of a loss-of-function strain, we have demonstrated that the mar1Δ mutant has an aberrant cell surface and a defect in polysaccharide capsule attachment, resulting in attenuated virulence.
30077416	6	0	theme	MHC	1213:1215	arg1	molecules					1225:1233	MHC class I molecules	1213:1233	MHC class I molecules that are associated with a broader diversity of oligosaccharides attached compared with tapasin	1213:1329	The distinction in glycan specificity between the two peptide editors suggests that TAPBPR may bind to MHC class I molecules that are associated with a broader diversity of oligosaccharides attached compared with tapasin.
30077416	6	1	theme	class	1217:1221	arg1	molecules					1225:1233	MHC class I molecules	1213:1233	MHC class I molecules that are associated with a broader diversity of oligosaccharides attached compared with tapasin	1213:1329	The distinction in glycan specificity between the two peptide editors suggests that TAPBPR may bind to MHC class I molecules that are associated with a broader diversity of oligosaccharides attached compared with tapasin.
30077416	5	2	theme	class	1023:1027	arg1	molecules					1031:1039	MHC class I molecules	1019:1039	MHC class I molecules	1019:1039	Furthermore, we found that glycosylated HLA-A2 preferentially interacts with tapasin rather than TAPBPR, possibly explaining, in part, why MHC class I molecules bind efficiently to tapasin in the face of an alternative chaperone.
30077416	5	3	gly	glycosylated	907:918	arg1	HLA-A2					920:925	glycosylated HLA-A2	907:925	glycosylated HLA-A2	907:925	Furthermore, we found that glycosylated HLA-A2 preferentially interacts with tapasin rather than TAPBPR, possibly explaining, in part, why MHC class I molecules bind efficiently to tapasin in the face of an alternative chaperone.
30077416	7	4	theme	TAPBPR	1381:1386	arg1	ability					1370:1376	the ability	1366:1376	the ability of TAPBPR to interact with MHC class I molecules outside of the ER	1366:1443	This may explain, to some extent, the ability of TAPBPR to interact with MHC class I molecules outside of the ER.
30077416	7	5	theme	MHC	1405:1407	arg1	molecules					1417:1425	MHC class I molecules	1405:1425	MHC class I molecules outside of the ER	1405:1443	This may explain, to some extent, the ability of TAPBPR to interact with MHC class I molecules outside of the ER.
30077416	1	6	theme	N-linked	152:159	arg1	glycan					161:166	the N-linked glycan	148:166	the N-linked glycan on MHC class I molecules	148:191	We recently discovered that TAPBPR promotes reglucosylation of the N-linked glycan on MHC class I molecules, a modification that restores their recognition by calreticulin and reincorporation into the peptide-loading complex.
30077416	1	6	theme	N-linked	152:159	arg1	modification					196:207	a modification	194:207	a modification that restores their recognition by calreticulin and reincorporation into the peptide-loading complex	194:308	We recently discovered that TAPBPR promotes reglucosylation of the N-linked glycan on MHC class I molecules, a modification that restores their recognition by calreticulin and reincorporation into the peptide-loading complex.
30077416	2	7	theme	MHC	501:503	arg1	class					505:509	MHC class I	501:511	MHC class I	501:511	We wondered whether TAPBPR displayed some degree of glycan specificity, as is known to be the case for tapasin via its interaction with calreticulin & ERp57, or whether its interaction with MHC class I was glycan independent.
30077416	4	8	theme	TAPBPR	826:831	arg1	interaction					845:855	the TAPBPR:MHC class I interaction	822:855	the TAPBPR:MHC class I interaction	822:855	In contrast to tapasin, TAPBPR bound strongly to MHC class I molecules that lacked N-linked glycosylation, suggesting that the TAPBPR:MHC class I interaction is glycan independent.
30077416	4	8	theme	TAPBPR	826:831	arg1	independent					867:877	independent	867:877	independent	867:877	In contrast to tapasin, TAPBPR bound strongly to MHC class I molecules that lacked N-linked glycosylation, suggesting that the TAPBPR:MHC class I interaction is glycan independent.
30077416	5	9	theme	chaperone	1099:1107	arg1	face					1076:1079	the face	1072:1079	the face of an alternative chaperone	1072:1107	Furthermore, we found that glycosylated HLA-A2 preferentially interacts with tapasin rather than TAPBPR, possibly explaining, in part, why MHC class I molecules bind efficiently to tapasin in the face of an alternative chaperone.
30077416	0	10	theme	glycosylation	70:82	arg1	absence					59:65	the absence	55:65	the absence of glycosylation	55:82	Preferential interaction of MHC class I with TAPBPR in the absence of glycosylation.
30077416	3	11	theme	glycosylation	665:677	arg1	sequence					689:696	disrupted NxS/T glycosylation consensus sequence	649:696	disrupted NxS/T glycosylation consensus sequence	649:696	Here, we explored this by comparing the ability of TAPBPR to bind to MHC class I containing either an intact or disrupted NxS/T glycosylation consensus sequence.
30077416	1	12	from	reglucosylation	129:143	arg1	molecules					183:191	MHC class I molecules	171:191	MHC class I molecules	171:191	We recently discovered that TAPBPR promotes reglucosylation of the N-linked glycan on MHC class I molecules, a modification that restores their recognition by calreticulin and reincorporation into the peptide-loading complex.
30077416	3	13	theme	consensus	679:687	arg1	sequence					689:696	disrupted NxS/T glycosylation consensus sequence	649:696	disrupted NxS/T glycosylation consensus sequence	649:696	Here, we explored this by comparing the ability of TAPBPR to bind to MHC class I containing either an intact or disrupted NxS/T glycosylation consensus sequence.
30077416	4	14	theme	N-linked	782:789	arg1	glycosylation					791:803	N-linked glycosylation	782:803	N-linked glycosylation	782:803	In contrast to tapasin, TAPBPR bound strongly to MHC class I molecules that lacked N-linked glycosylation, suggesting that the TAPBPR:MHC class I interaction is glycan independent.
30077416	6	15	from	distinction	1114:1124	arg1	specificity					1136:1146	glycan specificity	1129:1146	glycan specificity	1129:1146	The distinction in glycan specificity between the two peptide editors suggests that TAPBPR may bind to MHC class I molecules that are associated with a broader diversity of oligosaccharides attached compared with tapasin.
30077416	5	16	theme	glycosylated	907:918	arg1	HLA-A2					920:925	glycosylated HLA-A2	907:925	glycosylated HLA-A2	907:925	Furthermore, we found that glycosylated HLA-A2 preferentially interacts with tapasin rather than TAPBPR, possibly explaining, in part, why MHC class I molecules bind efficiently to tapasin in the face of an alternative chaperone.
30077416	3	17	contain	containing	618:627	arg1	class					610:614	MHC class I	606:616	MHC class I containing either an intact or disrupted NxS/T glycosylation consensus sequence	606:696	Here, we explored this by comparing the ability of TAPBPR to bind to MHC class I containing either an intact or disrupted NxS/T glycosylation consensus sequence.
30077416	3	17	contain	containing	618:627	arg2	sequence					689:696	disrupted NxS/T glycosylation consensus sequence	649:696	disrupted NxS/T glycosylation consensus sequence	649:696	Here, we explored this by comparing the ability of TAPBPR to bind to MHC class I containing either an intact or disrupted NxS/T glycosylation consensus sequence.
30077416	3	17	contain	containing	618:627	arg2	intact					639:644	intact	639:644	intact	639:644	Here, we explored this by comparing the ability of TAPBPR to bind to MHC class I containing either an intact or disrupted NxS/T glycosylation consensus sequence.
30077416	1	18	theme	glycan	161:166	arg1	reglucosylation					129:143	reglucosylation	129:143	reglucosylation of the N-linked glycan on MHC class I molecules, a modification that restores their recognition by calreticulin and reincorporation into the peptide-loading complex	129:308	We recently discovered that TAPBPR promotes reglucosylation of the N-linked glycan on MHC class I molecules, a modification that restores their recognition by calreticulin and reincorporation into the peptide-loading complex.
30077416	6	19	theme	broader	1262:1268	arg1	diversity					1270:1278	a broader diversity	1260:1278	a broader diversity of oligosaccharides attached compared with tapasin	1260:1329	The distinction in glycan specificity between the two peptide editors suggests that TAPBPR may bind to MHC class I molecules that are associated with a broader diversity of oligosaccharides attached compared with tapasin.
30077416	3	20	theme	disrupted	649:657	arg1	sequence					689:696	disrupted NxS/T glycosylation consensus sequence	649:696	disrupted NxS/T glycosylation consensus sequence	649:696	Here, we explored this by comparing the ability of TAPBPR to bind to MHC class I containing either an intact or disrupted NxS/T glycosylation consensus sequence.
30077416	2	21	with	interaction	484:494	arg1	class					505:509	MHC class I	501:511	MHC class I	501:511	We wondered whether TAPBPR displayed some degree of glycan specificity, as is known to be the case for tapasin via its interaction with calreticulin & ERp57, or whether its interaction with MHC class I was glycan independent.
30077416	3	22	theme	NxS/T	659:663	arg1	sequence					689:696	disrupted NxS/T glycosylation consensus sequence	649:696	disrupted NxS/T glycosylation consensus sequence	649:696	Here, we explored this by comparing the ability of TAPBPR to bind to MHC class I containing either an intact or disrupted NxS/T glycosylation consensus sequence.
30077416	1	23	theme	MHC	171:173	arg1	molecules					183:191	MHC class I molecules	171:191	MHC class I molecules	171:191	We recently discovered that TAPBPR promotes reglucosylation of the N-linked glycan on MHC class I molecules, a modification that restores their recognition by calreticulin and reincorporation into the peptide-loading complex.
30077416	0	24	theme	Preferential	0:11	arg1	interaction					13:23	Preferential interaction	0:23	Preferential interaction of MHC class I with TAPBPR	0:50	Preferential interaction of MHC class I with TAPBPR in the absence of glycosylation.
30077416	5	25	theme	alternative	1087:1097	arg1	chaperone					1099:1107	an alternative chaperone	1084:1107	an alternative chaperone	1084:1107	Furthermore, we found that glycosylated HLA-A2 preferentially interacts with tapasin rather than TAPBPR, possibly explaining, in part, why MHC class I molecules bind efficiently to tapasin in the face of an alternative chaperone.
30077416	1	26	theme	class	175:179	arg1	molecules					183:191	MHC class I molecules	171:191	MHC class I molecules	171:191	We recently discovered that TAPBPR promotes reglucosylation of the N-linked glycan on MHC class I molecules, a modification that restores their recognition by calreticulin and reincorporation into the peptide-loading complex.
30077416	0	27	theme	MHC	28:30	arg1	class					32:36	MHC class I	28:38	MHC class I	28:38	Preferential interaction of MHC class I with TAPBPR in the absence of glycosylation.
30077416	4	28	theme	MHC	748:750	arg1	molecules					760:768	MHC class I molecules	748:768	MHC class I molecules that lacked N-linked glycosylation	748:803	In contrast to tapasin, TAPBPR bound strongly to MHC class I molecules that lacked N-linked glycosylation, suggesting that the TAPBPR:MHC class I interaction is glycan independent.
30077416	6	29	theme	peptide	1164:1170	arg1	editors					1172:1178	the two peptide editors	1156:1178	the two peptide editors	1156:1178	The distinction in glycan specificity between the two peptide editors suggests that TAPBPR may bind to MHC class I molecules that are associated with a broader diversity of oligosaccharides attached compared with tapasin.
30077416	1	30	link	N-linked	152:159	arg1	glycan					161:166	the N-linked glycan	148:166	the N-linked glycan on MHC class I molecules	148:191	We recently discovered that TAPBPR promotes reglucosylation of the N-linked glycan on MHC class I molecules, a modification that restores their recognition by calreticulin and reincorporation into the peptide-loading complex.
30077416	1	30	link	N-linked	152:159	arg1	modification					196:207	a modification	194:207	a modification that restores their recognition by calreticulin and reincorporation into the peptide-loading complex	194:308	We recently discovered that TAPBPR promotes reglucosylation of the N-linked glycan on MHC class I molecules, a modification that restores their recognition by calreticulin and reincorporation into the peptide-loading complex.
30077416	2	31	theme	&	460:460	arg1	ERp57					462:466	calreticulin & ERp57	447:466	calreticulin & ERp57	447:466	We wondered whether TAPBPR displayed some degree of glycan specificity, as is known to be the case for tapasin via its interaction with calreticulin & ERp57, or whether its interaction with MHC class I was glycan independent.
30077416	1	32	theme	peptide-loading	286:300	arg1	complex					302:308	the peptide-loading complex	282:308	the peptide-loading complex	282:308	We recently discovered that TAPBPR promotes reglucosylation of the N-linked glycan on MHC class I molecules, a modification that restores their recognition by calreticulin and reincorporation into the peptide-loading complex.
30077416	4	33	theme	I	758:758	arg1	molecules					760:768	MHC class I molecules	748:768	MHC class I molecules that lacked N-linked glycosylation	748:803	In contrast to tapasin, TAPBPR bound strongly to MHC class I molecules that lacked N-linked glycosylation, suggesting that the TAPBPR:MHC class I interaction is glycan independent.
30077416	0	34	theme	class	32:36	arg1	interaction					13:23	Preferential interaction	0:23	Preferential interaction of MHC class I with TAPBPR	0:50	Preferential interaction of MHC class I with TAPBPR in the absence of glycosylation.
30077416	2	35	theme	calreticulin	447:458	arg1	ERp57					462:466	calreticulin & ERp57	447:466	calreticulin & ERp57	447:466	We wondered whether TAPBPR displayed some degree of glycan specificity, as is known to be the case for tapasin via its interaction with calreticulin & ERp57, or whether its interaction with MHC class I was glycan independent.
30077416	3	36	theme	TAPBPR	588:593	arg1	ability					577:583	the ability	573:583	the ability of TAPBPR to bind to MHC class I containing either an intact or disrupted NxS/T glycosylation consensus sequence	573:696	Here, we explored this by comparing the ability of TAPBPR to bind to MHC class I containing either an intact or disrupted NxS/T glycosylation consensus sequence.
30077416	4	37	theme	class	752:756	arg1	molecules					760:768	MHC class I molecules	748:768	MHC class I molecules that lacked N-linked glycosylation	748:803	In contrast to tapasin, TAPBPR bound strongly to MHC class I molecules that lacked N-linked glycosylation, suggesting that the TAPBPR:MHC class I interaction is glycan independent.
30077416	4	38	link	N-linked	782:789	arg1	glycosylation					791:803	N-linked glycosylation	782:803	N-linked glycosylation	782:803	In contrast to tapasin, TAPBPR bound strongly to MHC class I molecules that lacked N-linked glycosylation, suggesting that the TAPBPR:MHC class I interaction is glycan independent.
30077416	6	39	theme	oligosaccharides	1283:1298	arg1	diversity					1270:1278	a broader diversity	1260:1278	a broader diversity of oligosaccharides attached compared with tapasin	1260:1329	The distinction in glycan specificity between the two peptide editors suggests that TAPBPR may bind to MHC class I molecules that are associated with a broader diversity of oligosaccharides attached compared with tapasin.
30077416	4	40	theme	MHC	833:835	arg1	class					837:841	MHC class I	833:843	the TAPBPR:MHC class I interaction	822:855	In contrast to tapasin, TAPBPR bound strongly to MHC class I molecules that lacked N-linked glycosylation, suggesting that the TAPBPR:MHC class I interaction is glycan independent.
30077416	5	41	theme	MHC	1019:1021	arg1	molecules					1031:1039	MHC class I molecules	1019:1039	MHC class I molecules	1019:1039	Furthermore, we found that glycosylated HLA-A2 preferentially interacts with tapasin rather than TAPBPR, possibly explaining, in part, why MHC class I molecules bind efficiently to tapasin in the face of an alternative chaperone.
30077416	7	42	theme	I	1415:1415	arg1	molecules					1417:1425	MHC class I molecules	1405:1425	MHC class I molecules outside of the ER	1405:1443	This may explain, to some extent, the ability of TAPBPR to interact with MHC class I molecules outside of the ER.
30077416	0	43	with	interaction	13:23	arg1	TAPBPR					45:50	TAPBPR	45:50	TAPBPR	45:50	Preferential interaction of MHC class I with TAPBPR in the absence of glycosylation.
30077416	6	44	theme	I	1223:1223	arg1	molecules					1225:1233	MHC class I molecules	1213:1233	MHC class I molecules that are associated with a broader diversity of oligosaccharides attached compared with tapasin	1213:1329	The distinction in glycan specificity between the two peptide editors suggests that TAPBPR may bind to MHC class I molecules that are associated with a broader diversity of oligosaccharides attached compared with tapasin.
30077416	1	45	from	molecules	183:191	arg1	reglucosylation					129:143	reglucosylation	129:143	reglucosylation of the N-linked glycan on MHC class I molecules, a modification that restores their recognition by calreticulin and reincorporation into the peptide-loading complex	129:308	We recently discovered that TAPBPR promotes reglucosylation of the N-linked glycan on MHC class I molecules, a modification that restores their recognition by calreticulin and reincorporation into the peptide-loading complex.
30077416	4	46	theme	class	837:841	arg1	interaction					845:855	the TAPBPR:MHC class I interaction	822:855	the TAPBPR:MHC class I interaction	822:855	In contrast to tapasin, TAPBPR bound strongly to MHC class I molecules that lacked N-linked glycosylation, suggesting that the TAPBPR:MHC class I interaction is glycan independent.
30077416	4	46	theme	class	837:841	arg1	independent					867:877	independent	867:877	independent	867:877	In contrast to tapasin, TAPBPR bound strongly to MHC class I molecules that lacked N-linked glycosylation, suggesting that the TAPBPR:MHC class I interaction is glycan independent.
30077416	3	47	theme	MHC	606:608	arg1	class					610:614	MHC class I	606:616	MHC class I containing either an intact or disrupted NxS/T glycosylation consensus sequence	606:696	Here, we explored this by comparing the ability of TAPBPR to bind to MHC class I containing either an intact or disrupted NxS/T glycosylation consensus sequence.
30077416	6	48	theme	glycan	1129:1134	arg1	specificity					1136:1146	glycan specificity	1129:1146	glycan specificity	1129:1146	The distinction in glycan specificity between the two peptide editors suggests that TAPBPR may bind to MHC class I molecules that are associated with a broader diversity of oligosaccharides attached compared with tapasin.
30077416	7	49	theme	class	1409:1413	arg1	molecules					1417:1425	MHC class I molecules	1405:1425	MHC class I molecules outside of the ER	1405:1443	This may explain, to some extent, the ability of TAPBPR to interact with MHC class I molecules outside of the ER.
30077416	0	50	dep	interaction	13:23	arg1	absence					59:65	the absence	55:65	the absence of glycosylation	55:82	Preferential interaction of MHC class I with TAPBPR in the absence of glycosylation.
30077416	2	51	with	interaction	430:440	arg1	ERp57					462:466	calreticulin & ERp57	447:466	calreticulin & ERp57	447:466	We wondered whether TAPBPR displayed some degree of glycan specificity, as is known to be the case for tapasin via its interaction with calreticulin & ERp57, or whether its interaction with MHC class I was glycan independent.
30077416	2	52	theme	specificity	370:380	arg1	degree					353:358	some degree	348:358	some degree of glycan specificity	348:380	We wondered whether TAPBPR displayed some degree of glycan specificity, as is known to be the case for tapasin via its interaction with calreticulin & ERp57, or whether its interaction with MHC class I was glycan independent.
30077416	1	53	from	glycan	161:166	arg1	molecules					183:191	MHC class I molecules	171:191	MHC class I molecules	171:191	We recently discovered that TAPBPR promotes reglucosylation of the N-linked glycan on MHC class I molecules, a modification that restores their recognition by calreticulin and reincorporation into the peptide-loading complex.
30077416	2	54	theme	glycan	363:368	arg1	specificity					370:380	glycan specificity	363:380	glycan specificity	363:380	We wondered whether TAPBPR displayed some degree of glycan specificity, as is known to be the case for tapasin via its interaction with calreticulin & ERp57, or whether its interaction with MHC class I was glycan independent.
31771941	4	0	theme	bacterial	904:912	arg1	composition					914:924	bacterial composition	904:924	bacterial composition	904:924	Using a model of inflammation-induced colorectal cancer, we found that Lactobacillus species are lost more quickly in wild-type (WT) mice than TLR6-deficient mice resulting in overall differences in bacterial composition.
31771941	3	1	theme	specific	541:548	arg1	receptors					557:565	specific innate receptors	541:565	specific innate receptors	541:565	However, the role that specific innate receptors may play in the development of chronic inflammation and their impact on the composition of the colonic microbiota is not well understood.
31771941	8	2	theme	innate	1566:1571	arg1	signals					1580:1586	innate immune signals	1566:1586	innate immune signals	1566:1586	Altogether, these data suggest that innate immune signals can shape the composition of the microbiota under chronic inflammatory conditions, bias the cytokine milieu of the tissue microenvironment, and influence the response to microbiota-associated therapies.
31771941	0	3	theme	Microbiota	84:93	arg1	Impacts					57:63	Impacts	57:63	Impacts	57:63	Toll-like Receptor-6 Signaling Prevents Inflammation and Impacts Composition of the Microbiota During Inflammation-Induced Colorectal Cancer.
31771941	0	3	theme	Microbiota	84:93	arg1	Inflammation					40:51	Inflammation	40:51	Inflammation	40:51	Toll-like Receptor-6 Signaling Prevents Inflammation and Impacts Composition of the Microbiota During Inflammation-Induced Colorectal Cancer.
31771941	7	4	theme	bacterial	1507:1515	arg1	composition					1517:1527	bacterial composition	1507:1527	bacterial composition	1507:1527	We found that the TLR6-dependent effects of Lactobacillus could be dissociated from one another via the involvement of IL10, which was necessary to dampen the inflammatory microenvironment, but had no effect on bacterial composition.
31771941	5	5	theme	longer	939:944	arg1	retention					946:954	the longer retention	935:954	the longer retention of Lactobacillus	935:971	Despite the longer retention of Lactobacillus, the TLR6-deficient mice presented with more tumors and a worse overall outcome.
31771941	2	6	theme	immune	382:387	arg1	responses					389:397	aberrant immune responses	373:397	aberrant immune responses in the intestine	373:414	Toll-like receptors play an important role in preventing aberrant immune responses in the intestine by sensing endogenous commensal microbiota and delivering important regulatory signals to the tissue.
31771941	0	7	theme	Colorectal	123:132	arg1	Cancer					134:139	Inflammation-Induced Colorectal Cancer	102:139	Inflammation-Induced Colorectal Cancer	102:139	Toll-like Receptor-6 Signaling Prevents Inflammation and Impacts Composition of the Microbiota During Inflammation-Induced Colorectal Cancer.
31771941	4	8	theme	wild-type	823:831	arg1	mice					838:841	wild-type (WT) mice	823:841	wild-type (WT) mice than TLR6-deficient mice resulting in overall differences in bacterial composition	823:924	Using a model of inflammation-induced colorectal cancer, we found that Lactobacillus species are lost more quickly in wild-type (WT) mice than TLR6-deficient mice resulting in overall differences in bacterial composition.
31771941	7	9	theme	IL10	1415:1418	arg1	involvement					1400:1410	the involvement	1396:1410	the involvement	1396:1410	We found that the TLR6-dependent effects of Lactobacillus could be dissociated from one another via the involvement of IL10, which was necessary to dampen the inflammatory microenvironment, but had no effect on bacterial composition.
31771941	7	9	theme	IL10	1415:1418	arg1	necessary					1431:1439	necessary	1431:1439	necessary	1431:1439	We found that the TLR6-dependent effects of Lactobacillus could be dissociated from one another via the involvement of IL10, which was necessary to dampen the inflammatory microenvironment, but had no effect on bacterial composition.
31771941	2	10	theme	Toll-like	316:324	arg1	receptors					326:334	Toll-like receptors	316:334	Toll-like receptors	316:334	Toll-like receptors play an important role in preventing aberrant immune responses in the intestine by sensing endogenous commensal microbiota and delivering important regulatory signals to the tissue.
31771941	3	11	from	development	583:593	arg1	composition					643:653	the composition	639:653	the composition of the colonic microbiota	639:679	However, the role that specific innate receptors may play in the development of chronic inflammation and their impact on the composition of the colonic microbiota is not well understood.
31771941	7	12	dep	one	1380:1382	arg1	another					1384:1390	another	1384:1390	another	1384:1390	We found that the TLR6-dependent effects of Lactobacillus could be dissociated from one another via the involvement of IL10, which was necessary to dampen the inflammatory microenvironment, but had no effect on bacterial composition.
31771941	2	13	theme	important	344:352	arg1	role					354:357	an important role	341:357	an important role	341:357	Toll-like receptors play an important role in preventing aberrant immune responses in the intestine by sensing endogenous commensal microbiota and delivering important regulatory signals to the tissue.
31771941	4	14	from	differences	889:899	arg1	composition					914:924	bacterial composition	904:924	bacterial composition	904:924	Using a model of inflammation-induced colorectal cancer, we found that Lactobacillus species are lost more quickly in wild-type (WT) mice than TLR6-deficient mice resulting in overall differences in bacterial composition.
31771941	4	15	theme	colorectal	743:752	arg1	cancer					754:759	inflammation-induced colorectal cancer	722:759	inflammation-induced colorectal cancer	722:759	Using a model of inflammation-induced colorectal cancer, we found that Lactobacillus species are lost more quickly in wild-type (WT) mice than TLR6-deficient mice resulting in overall differences in bacterial composition.
31771941	8	16	theme	cytokine	1680:1687	arg1	milieu					1689:1694	the cytokine milieu	1676:1694	the cytokine milieu of the tissue microenvironment	1676:1725	Altogether, these data suggest that innate immune signals can shape the composition of the microbiota under chronic inflammatory conditions, bias the cytokine milieu of the tissue microenvironment, and influence the response to microbiota-associated therapies.
31771941	4	17	theme	inflammation-induced	722:741	arg1	cancer					754:759	inflammation-induced colorectal cancer	722:759	inflammation-induced colorectal cancer	722:759	Using a model of inflammation-induced colorectal cancer, we found that Lactobacillus species are lost more quickly in wild-type (WT) mice than TLR6-deficient mice resulting in overall differences in bacterial composition.
31771941	7	18	theme	TLR6-dependent	1314:1327	arg1	effects					1329:1335	the TLR6-dependent effects	1310:1335	the TLR6-dependent effects of Lactobacillus	1310:1352	We found that the TLR6-dependent effects of Lactobacillus could be dissociated from one another via the involvement of IL10, which was necessary to dampen the inflammatory microenvironment, but had no effect on bacterial composition.
31771941	4	19	theme	Lactobacillus	776:788	arg1	species					790:796	Lactobacillus species	776:796	Lactobacillus species	776:796	Using a model of inflammation-induced colorectal cancer, we found that Lactobacillus species are lost more quickly in wild-type (WT) mice than TLR6-deficient mice resulting in overall differences in bacterial composition.
31771941	6	20	theme	WT	1222:1223	arg1	mice					1225:1228	WT mice	1222:1228	WT mice	1222:1228	Restoration of the lost Lactobacillus species suppressed inflammation, reduced tumor number, and prevented change in the abundance of Proteobacteria only when given to WT mice, indicating the effect of these Lactobacillus are TLR6 dependent.
31771941	2	21	theme	endogenous	427:436	arg1	microbiota					448:457	endogenous commensal microbiota	427:457	endogenous commensal microbiota	427:457	Toll-like receptors play an important role in preventing aberrant immune responses in the intestine by sensing endogenous commensal microbiota and delivering important regulatory signals to the tissue.
31771941	2	22	from	responses	389:397	arg1	intestine					406:414	the intestine	402:414	the intestine	402:414	Toll-like receptors play an important role in preventing aberrant immune responses in the intestine by sensing endogenous commensal microbiota and delivering important regulatory signals to the tissue.
31771941	5	23	theme	TLR6-deficient	978:991	arg1	mice					993:996	the TLR6-deficient mice	974:996	the TLR6-deficient mice	974:996	Despite the longer retention of Lactobacillus, the TLR6-deficient mice presented with more tumors and a worse overall outcome.
31771941	6	24	theme	tumor	1133:1137	arg1	number					1139:1144	tumor number	1133:1144	tumor number	1133:1144	Restoration of the lost Lactobacillus species suppressed inflammation, reduced tumor number, and prevented change in the abundance of Proteobacteria only when given to WT mice, indicating the effect of these Lactobacillus are TLR6 dependent.
31771941	0	25	theme	Receptor-6	10:19	arg1	Signaling					21:29	Toll-like Receptor-6 Signaling	0:29	Toll-like Receptor-6 Signaling	0:29	Toll-like Receptor-6 Signaling Prevents Inflammation and Impacts Composition of the Microbiota During Inflammation-Induced Colorectal Cancer.
31771941	8	26	theme	immune	1573:1578	arg1	signals					1580:1586	innate immune signals	1566:1586	innate immune signals	1566:1586	Altogether, these data suggest that innate immune signals can shape the composition of the microbiota under chronic inflammatory conditions, bias the cytokine milieu of the tissue microenvironment, and influence the response to microbiota-associated therapies.
31771941	3	27	theme	inflammation	606:617	arg1	development					583:593	the development	579:593	the development of chronic inflammation	579:617	However, the role that specific innate receptors may play in the development of chronic inflammation and their impact on the composition of the colonic microbiota is not well understood.
31771941	3	27	theme	inflammation	606:617	arg1	impact					629:634	their impact	623:634	their impact on the composition of the colonic microbiota	623:679	However, the role that specific innate receptors may play in the development of chronic inflammation and their impact on the composition of the colonic microbiota is not well understood.
31771941	8	28	theme	microbiota-associated	1758:1778	arg1	therapies					1780:1788	microbiota-associated therapies	1758:1788	microbiota-associated therapies	1758:1788	Altogether, these data suggest that innate immune signals can shape the composition of the microbiota under chronic inflammatory conditions, bias the cytokine milieu of the tissue microenvironment, and influence the response to microbiota-associated therapies.
31771941	0	29	theme	Toll-like	0:8	arg1	Signaling					21:29	Toll-like Receptor-6 Signaling	0:29	Toll-like Receptor-6 Signaling	0:29	Toll-like Receptor-6 Signaling Prevents Inflammation and Impacts Composition of the Microbiota During Inflammation-Induced Colorectal Cancer.
31771941	1	30	theme	colorectal	297:306	arg1	cancer					308:313	colorectal cancer	297:313	colorectal cancer	297:313	Tightly regulated immune responses must occur in the intestine to avoid unwanted inflammation, which may cause chronic sequela leading to diseases such as colorectal cancer.
31771941	4	31	theme	overall	881:887	arg1	differences					889:899	overall differences	881:899	overall differences in bacterial composition	881:924	Using a model of inflammation-induced colorectal cancer, we found that Lactobacillus species are lost more quickly in wild-type (WT) mice than TLR6-deficient mice resulting in overall differences in bacterial composition.
31771941	5	32	theme	overall	1037:1043	arg1	outcome					1045:1051	a worse overall outcome	1029:1051	a worse overall outcome	1029:1051	Despite the longer retention of Lactobacillus, the TLR6-deficient mice presented with more tumors and a worse overall outcome.
31771941	1	33	theme	unwanted	214:221	arg1	inflammation					223:234	unwanted inflammation	214:234	unwanted inflammation	214:234	Tightly regulated immune responses must occur in the intestine to avoid unwanted inflammation, which may cause chronic sequela leading to diseases such as colorectal cancer.
31771941	2	34	theme	regulatory	484:493	arg1	signals					495:501	important regulatory signals	474:501	important regulatory signals to the tissue	474:515	Toll-like receptors play an important role in preventing aberrant immune responses in the intestine by sensing endogenous commensal microbiota and delivering important regulatory signals to the tissue.
31771941	4	35	theme	TLR6-deficient	848:861	arg1	mice					863:866	TLR6-deficient mice	848:866	TLR6-deficient mice resulting in overall differences in bacterial composition	848:924	Using a model of inflammation-induced colorectal cancer, we found that Lactobacillus species are lost more quickly in wild-type (WT) mice than TLR6-deficient mice resulting in overall differences in bacterial composition.
31771941	3	36	from	impact	629:634	arg1	composition					643:653	the composition	639:653	the composition of the colonic microbiota	639:679	However, the role that specific innate receptors may play in the development of chronic inflammation and their impact on the composition of the colonic microbiota is not well understood.
31771941	5	37	theme	more	1013:1016	arg1	tumors					1018:1023	more tumors	1013:1023	more tumors	1013:1023	Despite the longer retention of Lactobacillus, the TLR6-deficient mice presented with more tumors and a worse overall outcome.
31771941	2	38	theme	important	474:482	arg1	signals					495:501	important regulatory signals	474:501	important regulatory signals to the tissue	474:515	Toll-like receptors play an important role in preventing aberrant immune responses in the intestine by sensing endogenous commensal microbiota and delivering important regulatory signals to the tissue.
31771941	7	39	theme	inflammatory	1455:1466	arg1	microenvironment					1468:1483	the inflammatory microenvironment	1451:1483	the inflammatory microenvironment	1451:1483	We found that the TLR6-dependent effects of Lactobacillus could be dissociated from one another via the involvement of IL10, which was necessary to dampen the inflammatory microenvironment, but had no effect on bacterial composition.
31771941	3	40	theme	chronic	598:604	arg1	inflammation					606:617	chronic inflammation	598:617	chronic inflammation	598:617	However, the role that specific innate receptors may play in the development of chronic inflammation and their impact on the composition of the colonic microbiota is not well understood.
31771941	7	41	theme	Lactobacillus	1340:1352	arg1	effects					1329:1335	the TLR6-dependent effects	1310:1335	the TLR6-dependent effects of Lactobacillus	1310:1352	We found that the TLR6-dependent effects of Lactobacillus could be dissociated from one another via the involvement of IL10, which was necessary to dampen the inflammatory microenvironment, but had no effect on bacterial composition.
31771941	4	42	theme	cancer	754:759	arg1	model					713:717	a model	711:717	a model of inflammation-induced colorectal cancer	711:759	Using a model of inflammation-induced colorectal cancer, we found that Lactobacillus species are lost more quickly in wild-type (WT) mice than TLR6-deficient mice resulting in overall differences in bacterial composition.
31771941	4	43	dep	wild-type	823:831	arg1	WT					834:835	WT	834:835	WT	834:835	Using a model of inflammation-induced colorectal cancer, we found that Lactobacillus species are lost more quickly in wild-type (WT) mice than TLR6-deficient mice resulting in overall differences in bacterial composition.
31771941	6	44	theme	species	1092:1098	arg1	Restoration					1054:1064	Restoration	1054:1064	Restoration of the lost Lactobacillus species	1054:1098	Restoration of the lost Lactobacillus species suppressed inflammation, reduced tumor number, and prevented change in the abundance of Proteobacteria only when given to WT mice, indicating the effect of these Lactobacillus are TLR6 dependent.
31771941	3	45	theme	microbiota	670:679	arg1	composition					643:653	the composition	639:653	the composition of the colonic microbiota	639:679	However, the role that specific innate receptors may play in the development of chronic inflammation and their impact on the composition of the colonic microbiota is not well understood.
31771941	5	46	theme	Lactobacillus	959:971	arg1	retention					946:954	the longer retention	935:954	the longer retention of Lactobacillus	935:971	Despite the longer retention of Lactobacillus, the TLR6-deficient mice presented with more tumors and a worse overall outcome.
31771941	2	47	theme	commensal	438:446	arg1	microbiota					448:457	endogenous commensal microbiota	427:457	endogenous commensal microbiota	427:457	Toll-like receptors play an important role in preventing aberrant immune responses in the intestine by sensing endogenous commensal microbiota and delivering important regulatory signals to the tissue.
31771941	7	48	contain	had	1490:1492	arg1	involvement					1400:1410	the involvement	1396:1410	the involvement	1396:1410	We found that the TLR6-dependent effects of Lactobacillus could be dissociated from one another via the involvement of IL10, which was necessary to dampen the inflammatory microenvironment, but had no effect on bacterial composition.
31771941	7	48	contain	had	1490:1492	arg1	necessary					1431:1439	necessary	1431:1439	necessary	1431:1439	We found that the TLR6-dependent effects of Lactobacillus could be dissociated from one another via the involvement of IL10, which was necessary to dampen the inflammatory microenvironment, but had no effect on bacterial composition.
31771941	7	48	contain	had	1490:1492	arg2	effect					1497:1502	no effect	1494:1502	no effect	1494:1502	We found that the TLR6-dependent effects of Lactobacillus could be dissociated from one another via the involvement of IL10, which was necessary to dampen the inflammatory microenvironment, but had no effect on bacterial composition.
31771941	6	49	theme	Lactobacillus	1262:1274	arg1	effect					1246:1251	the effect	1242:1251	the effect of these Lactobacillus are TLR6 dependent	1242:1293	Restoration of the lost Lactobacillus species suppressed inflammation, reduced tumor number, and prevented change in the abundance of Proteobacteria only when given to WT mice, indicating the effect of these Lactobacillus are TLR6 dependent.
31771941	2	50	theme	aberrant	373:380	arg1	responses					389:397	aberrant immune responses	373:397	aberrant immune responses in the intestine	373:414	Toll-like receptors play an important role in preventing aberrant immune responses in the intestine by sensing endogenous commensal microbiota and delivering important regulatory signals to the tissue.
31771941	0	51	dep	Inflammation	40:51	arg1	Composition					65:75	Composition	65:75	Composition	65:75	Toll-like Receptor-6 Signaling Prevents Inflammation and Impacts Composition of the Microbiota During Inflammation-Induced Colorectal Cancer.
31771941	3	52	theme	innate	550:555	arg1	receptors					557:565	specific innate receptors	541:565	specific innate receptors	541:565	However, the role that specific innate receptors may play in the development of chronic inflammation and their impact on the composition of the colonic microbiota is not well understood.
31771941	1	53	theme	regulated	150:158	arg1	responses					167:175	Tightly regulated immune responses	142:175	Tightly regulated immune responses	142:175	Tightly regulated immune responses must occur in the intestine to avoid unwanted inflammation, which may cause chronic sequela leading to diseases such as colorectal cancer.
31771941	8	54	theme	microbiota	1621:1630	arg1	composition					1602:1612	the composition	1598:1612	the composition of the microbiota	1598:1630	Altogether, these data suggest that innate immune signals can shape the composition of the microbiota under chronic inflammatory conditions, bias the cytokine milieu of the tissue microenvironment, and influence the response to microbiota-associated therapies.
31771941	5	55	theme	worse	1031:1035	arg1	outcome					1045:1051	a worse overall outcome	1029:1051	a worse overall outcome	1029:1051	Despite the longer retention of Lactobacillus, the TLR6-deficient mice presented with more tumors and a worse overall outcome.
31771941	1	56	theme	immune	160:165	arg1	responses					167:175	Tightly regulated immune responses	142:175	Tightly regulated immune responses	142:175	Tightly regulated immune responses must occur in the intestine to avoid unwanted inflammation, which may cause chronic sequela leading to diseases such as colorectal cancer.
31771941	6	57	theme	lost	1073:1076	arg1	species					1092:1098	the lost Lactobacillus species	1069:1098	the lost Lactobacillus species	1069:1098	Restoration of the lost Lactobacillus species suppressed inflammation, reduced tumor number, and prevented change in the abundance of Proteobacteria only when given to WT mice, indicating the effect of these Lactobacillus are TLR6 dependent.
31771941	3	58	theme	colonic	662:668	arg1	microbiota					670:679	the colonic microbiota	658:679	the colonic microbiota	658:679	However, the role that specific innate receptors may play in the development of chronic inflammation and their impact on the composition of the colonic microbiota is not well understood.
31771941	8	59	theme	microenvironment	1710:1725	arg1	milieu					1689:1694	the cytokine milieu	1676:1694	the cytokine milieu of the tissue microenvironment	1676:1725	Altogether, these data suggest that innate immune signals can shape the composition of the microbiota under chronic inflammatory conditions, bias the cytokine milieu of the tissue microenvironment, and influence the response to microbiota-associated therapies.
31771941	1	60	theme	chronic	253:259	arg1	sequela					261:267	chronic sequela	253:267	chronic sequela leading to diseases such as colorectal cancer	253:313	Tightly regulated immune responses must occur in the intestine to avoid unwanted inflammation, which may cause chronic sequela leading to diseases such as colorectal cancer.
31771941	6	61	theme	Lactobacillus	1078:1090	arg1	species					1092:1098	the lost Lactobacillus species	1069:1098	the lost Lactobacillus species	1069:1098	Restoration of the lost Lactobacillus species suppressed inflammation, reduced tumor number, and prevented change in the abundance of Proteobacteria only when given to WT mice, indicating the effect of these Lactobacillus are TLR6 dependent.
31771941	6	62	dep	Lactobacillus	1262:1274	arg1	dependent					1285:1293	dependent	1285:1293	dependent	1285:1293	Restoration of the lost Lactobacillus species suppressed inflammation, reduced tumor number, and prevented change in the abundance of Proteobacteria only when given to WT mice, indicating the effect of these Lactobacillus are TLR6 dependent.
31771941	8	63	theme	tissue	1703:1708	arg1	microenvironment					1710:1725	the tissue microenvironment	1699:1725	the tissue microenvironment	1699:1725	Altogether, these data suggest that innate immune signals can shape the composition of the microbiota under chronic inflammatory conditions, bias the cytokine milieu of the tissue microenvironment, and influence the response to microbiota-associated therapies.
31771941	6	64	theme	Proteobacteria	1188:1201	arg1	abundance					1175:1183	the abundance	1171:1183	the abundance of Proteobacteria	1171:1201	Restoration of the lost Lactobacillus species suppressed inflammation, reduced tumor number, and prevented change in the abundance of Proteobacteria only when given to WT mice, indicating the effect of these Lactobacillus are TLR6 dependent.
31771941	8	65	theme	chronic	1638:1644	arg1	conditions					1659:1668	chronic inflammatory conditions	1638:1668	chronic inflammatory conditions	1638:1668	Altogether, these data suggest that innate immune signals can shape the composition of the microbiota under chronic inflammatory conditions, bias the cytokine milieu of the tissue microenvironment, and influence the response to microbiota-associated therapies.
31771941	6	66	from	change	1161:1166	arg1	abundance					1175:1183	the abundance	1171:1183	the abundance of Proteobacteria	1171:1201	Restoration of the lost Lactobacillus species suppressed inflammation, reduced tumor number, and prevented change in the abundance of Proteobacteria only when given to WT mice, indicating the effect of these Lactobacillus are TLR6 dependent.
31771941	8	67	theme	inflammatory	1646:1657	arg1	conditions					1659:1668	chronic inflammatory conditions	1638:1668	chronic inflammatory conditions	1638:1668	Altogether, these data suggest that innate immune signals can shape the composition of the microbiota under chronic inflammatory conditions, bias the cytokine milieu of the tissue microenvironment, and influence the response to microbiota-associated therapies.
31771941	0	68	theme	Inflammation-Induced	102:121	arg1	Cancer					134:139	Inflammation-Induced Colorectal Cancer	102:139	Inflammation-Induced Colorectal Cancer	102:139	Toll-like Receptor-6 Signaling Prevents Inflammation and Impacts Composition of the Microbiota During Inflammation-Induced Colorectal Cancer.
31880067	2	0	theme	gut	312:314	arg1	composition					327:337	gut microbiota composition	312:337	gut microbiota composition	312:337	Captivity and human source food have been shown to influence drastically the gut microbiota composition and function of wild animals.
31880067	6	1	theme	enriched	978:985	arg1	pathways					997:1004	enriched microbial pathways	978:1004	enriched microbial pathways involved in glycan biosynthesis and metabolism, transport and catabolism, and the digestive and endocrine systems	978:1118	Functional analysis revealed that wild macaques had enriched microbial pathways involved in glycan biosynthesis and metabolism, transport and catabolism, and the digestive and endocrine systems, while provisioned macaques were richer in pathways associated with signaling molecules and interaction, neurodegenerative diseases.
31880067	7	2	theme	provisioned	1332:1342	arg1	macaques					1344:1351	the provisioned macaques	1328:1351	the provisioned macaques	1328:1351	These differences were likely due to modification of the gut microbiota of the provisioned macaques to enable the digestion of new foods.
31880067	2	3	theme	human	249:253	arg1	food					262:265	human source food	249:265	human source food	249:265	Captivity and human source food have been shown to influence drastically the gut microbiota composition and function of wild animals.
31880067	3	4	theme	populations	445:455	arg1	microbiota					410:419	the gut microbiota	402:419	the gut microbiota of provisioned and wild populations of limestone-living rhesus macaques (Macaca mulatta)	402:508	Therefore, in the present study, the gut microbiota of provisioned and wild populations of limestone-living rhesus macaques (Macaca mulatta) were compared using high-throughput 16S rRNA sequencing and bioinformatic analyses.
31880067	3	5	theme	provisioned	424:434	arg1	populations					445:455	provisioned and wild populations	424:455	provisioned and wild populations of limestone-living rhesus macaques (Macaca mulatta)	424:508	Therefore, in the present study, the gut microbiota of provisioned and wild populations of limestone-living rhesus macaques (Macaca mulatta) were compared using high-throughput 16S rRNA sequencing and bioinformatic analyses.
31880067	0	6	theme	limestone	84:92	arg1	forest					94:99	a limestone forest	82:99	a limestone forest in southwest Guangxi, China	82:127	Gut microbiota of provisioned and wild rhesus macaques (Macaca mulatta) living in a limestone forest in southwest Guangxi, China.
31880067	5	7	theme	Provisioned	801:811	arg1	macaques					813:820	Provisioned macaques	801:820	Provisioned macaques	801:820	Provisioned macaques also showed a higher abundance of Firmicutes and a lower abundance of Bacteroidetes than wild macaques.
31880067	4	8	from	difference	723:732	arg1	evenness					741:748	the evenness	737:748	the evenness of the gut microbiota	737:770	The results indicated that provisioned macaques had a higher microbial richness than wild macaques, but there was no significant difference in the evenness of the gut microbiota between the two populations.
31880067	2	9	dep	composition	327:337	arg1	the					308:310	the	308:310	the	308:310	Captivity and human source food have been shown to influence drastically the gut microbiota composition and function of wild animals.
31880067	1	10	theme	gut	134:136	arg1	microbiota					138:147	The gut microbiota	130:147	The gut microbiota	130:147	The gut microbiota plays an important role in animal health and is strongly affected by the environment.
31880067	6	11	theme	wild	960:963	arg1	macaques					965:972	wild macaques	960:972	wild macaques	960:972	Functional analysis revealed that wild macaques had enriched microbial pathways involved in glycan biosynthesis and metabolism, transport and catabolism, and the digestive and endocrine systems, while provisioned macaques were richer in pathways associated with signaling molecules and interaction, neurodegenerative diseases.
31880067	6	12	theme	provisioned	1127:1137	arg1	macaques					1139:1146	provisioned macaques	1127:1146	provisioned macaques	1127:1146	Functional analysis revealed that wild macaques had enriched microbial pathways involved in glycan biosynthesis and metabolism, transport and catabolism, and the digestive and endocrine systems, while provisioned macaques were richer in pathways associated with signaling molecules and interaction, neurodegenerative diseases.
31880067	4	13	theme	provisioned	621:631	arg1	macaques					633:640	provisioned macaques	621:640	provisioned macaques	621:640	The results indicated that provisioned macaques had a higher microbial richness than wild macaques, but there was no significant difference in the evenness of the gut microbiota between the two populations.
31880067	6	14	theme	Functional	926:935	arg1	analysis					937:944	Functional analysis	926:944	Functional analysis	926:944	Functional analysis revealed that wild macaques had enriched microbial pathways involved in glycan biosynthesis and metabolism, transport and catabolism, and the digestive and endocrine systems, while provisioned macaques were richer in pathways associated with signaling molecules and interaction, neurodegenerative diseases.
31880067	7	15	theme	microbiota	1314:1323	arg1	modification					1290:1301	modification	1290:1301	modification of the gut microbiota of the provisioned macaques to enable the digestion of new foods	1290:1388	These differences were likely due to modification of the gut microbiota of the provisioned macaques to enable the digestion of new foods.
31880067	6	16	theme	neurodegenerative	1225:1241	arg1	interaction					1212:1222	interaction	1212:1222	interaction	1212:1222	Functional analysis revealed that wild macaques had enriched microbial pathways involved in glycan biosynthesis and metabolism, transport and catabolism, and the digestive and endocrine systems, while provisioned macaques were richer in pathways associated with signaling molecules and interaction, neurodegenerative diseases.
31880067	6	16	theme	neurodegenerative	1225:1241	arg1	diseases					1243:1250	neurodegenerative diseases	1225:1250	neurodegenerative diseases	1225:1250	Functional analysis revealed that wild macaques had enriched microbial pathways involved in glycan biosynthesis and metabolism, transport and catabolism, and the digestive and endocrine systems, while provisioned macaques were richer in pathways associated with signaling molecules and interaction, neurodegenerative diseases.
31880067	5	17	theme	higher	836:841	arg1	abundance					843:851	a higher abundance	834:851	a higher abundance of Firmicutes	834:865	Provisioned macaques also showed a higher abundance of Firmicutes and a lower abundance of Bacteroidetes than wild macaques.
31880067	0	18	theme	Gut	0:2	arg1	microbiota					4:13	Gut microbiota	0:13	Gut microbiota of provisioned and wild rhesus macaques (Macaca mulatta)	0:70	Gut microbiota of provisioned and wild rhesus macaques (Macaca mulatta) living in a limestone forest in southwest Guangxi, China.
31880067	5	19	theme	Firmicutes	856:865	arg1	abundance					879:887	a lower abundance	871:887	a lower abundance of Bacteroidetes	871:904	Provisioned macaques also showed a higher abundance of Firmicutes and a lower abundance of Bacteroidetes than wild macaques.
31880067	5	19	theme	Firmicutes	856:865	arg1	abundance					843:851	a higher abundance	834:851	a higher abundance of Firmicutes	834:865	Provisioned macaques also showed a higher abundance of Firmicutes and a lower abundance of Bacteroidetes than wild macaques.
31880067	0	20	theme	provisioned	18:28	arg1	mulatta					63:69	Macaca mulatta	56:69	Macaca mulatta	56:69	Gut microbiota of provisioned and wild rhesus macaques (Macaca mulatta) living in a limestone forest in southwest Guangxi, China.
31880067	0	20	theme	provisioned	18:28	arg1	macaques					46:53	provisioned and wild rhesus macaques	18:53	provisioned and wild rhesus macaques (Macaca mulatta)	18:70	Gut microbiota of provisioned and wild rhesus macaques (Macaca mulatta) living in a limestone forest in southwest Guangxi, China.
31880067	6	21	theme	signaling	1188:1196	arg1	molecules					1198:1206	signaling molecules	1188:1206	signaling molecules	1188:1206	Functional analysis revealed that wild macaques had enriched microbial pathways involved in glycan biosynthesis and metabolism, transport and catabolism, and the digestive and endocrine systems, while provisioned macaques were richer in pathways associated with signaling molecules and interaction, neurodegenerative diseases.
31880067	5	22	theme	Bacteroidetes	892:904	arg1	abundance					879:887	a lower abundance	871:887	a lower abundance of Bacteroidetes	871:904	Provisioned macaques also showed a higher abundance of Firmicutes and a lower abundance of Bacteroidetes than wild macaques.
31880067	5	22	theme	Bacteroidetes	892:904	arg1	abundance					843:851	a higher abundance	834:851	a higher abundance of Firmicutes	834:865	Provisioned macaques also showed a higher abundance of Firmicutes and a lower abundance of Bacteroidetes than wild macaques.
31880067	4	23	theme	significant	711:721	arg1	difference					723:732	no significant difference	708:732	no significant difference in the evenness of the gut microbiota between the two populations	708:798	The results indicated that provisioned macaques had a higher microbial richness than wild macaques, but there was no significant difference in the evenness of the gut microbiota between the two populations.
31880067	7	24	theme	foods	1384:1388	arg1	digestion					1367:1375	the digestion	1363:1375	the digestion of new foods	1363:1388	These differences were likely due to modification of the gut microbiota of the provisioned macaques to enable the digestion of new foods.
31880067	3	25	theme	gut	406:408	arg1	microbiota					410:419	the gut microbiota	402:419	the gut microbiota of provisioned and wild populations of limestone-living rhesus macaques (Macaca mulatta)	402:508	Therefore, in the present study, the gut microbiota of provisioned and wild populations of limestone-living rhesus macaques (Macaca mulatta) were compared using high-throughput 16S rRNA sequencing and bioinformatic analyses.
31880067	5	26	theme	wild	911:914	arg1	macaques					916:923	wild macaques	911:923	wild macaques	911:923	Provisioned macaques also showed a higher abundance of Firmicutes and a lower abundance of Bacteroidetes than wild macaques.
31880067	3	27	theme	macaques	484:491	arg1	populations					445:455	provisioned and wild populations	424:455	provisioned and wild populations of limestone-living rhesus macaques (Macaca mulatta)	424:508	Therefore, in the present study, the gut microbiota of provisioned and wild populations of limestone-living rhesus macaques (Macaca mulatta) were compared using high-throughput 16S rRNA sequencing and bioinformatic analyses.
31880067	2	28	theme	source	255:260	arg1	food					262:265	human source food	249:265	human source food	249:265	Captivity and human source food have been shown to influence drastically the gut microbiota composition and function of wild animals.
31880067	4	29	theme	microbial	655:663	arg1	richness					665:672	a higher microbial richness	646:672	a higher microbial richness	646:672	The results indicated that provisioned macaques had a higher microbial richness than wild macaques, but there was no significant difference in the evenness of the gut microbiota between the two populations.
31880067	0	30	theme	wild	34:37	arg1	mulatta					63:69	Macaca mulatta	56:69	Macaca mulatta	56:69	Gut microbiota of provisioned and wild rhesus macaques (Macaca mulatta) living in a limestone forest in southwest Guangxi, China.
31880067	0	30	theme	wild	34:37	arg1	macaques					46:53	provisioned and wild rhesus macaques	18:53	provisioned and wild rhesus macaques (Macaca mulatta)	18:70	Gut microbiota of provisioned and wild rhesus macaques (Macaca mulatta) living in a limestone forest in southwest Guangxi, China.
31880067	4	31	theme	wild	679:682	arg1	macaques					684:691	wild macaques	679:691	wild macaques	679:691	The results indicated that provisioned macaques had a higher microbial richness than wild macaques, but there was no significant difference in the evenness of the gut microbiota between the two populations.
31880067	3	32	theme	rRNA	550:553	arg1	sequencing					555:564	high-throughput 16S rRNA sequencing	530:564	high-throughput 16S rRNA sequencing	530:564	Therefore, in the present study, the gut microbiota of provisioned and wild populations of limestone-living rhesus macaques (Macaca mulatta) were compared using high-throughput 16S rRNA sequencing and bioinformatic analyses.
31880067	2	33	theme	wild	355:358	arg1	animals					360:366	wild animals	355:366	wild animals	355:366	Captivity and human source food have been shown to influence drastically the gut microbiota composition and function of wild animals.
31880067	6	34	theme	microbial	987:995	arg1	pathways					997:1004	enriched microbial pathways	978:1004	enriched microbial pathways involved in glycan biosynthesis and metabolism, transport and catabolism, and the digestive and endocrine systems	978:1118	Functional analysis revealed that wild macaques had enriched microbial pathways involved in glycan biosynthesis and metabolism, transport and catabolism, and the digestive and endocrine systems, while provisioned macaques were richer in pathways associated with signaling molecules and interaction, neurodegenerative diseases.
31880067	7	35	theme	new	1380:1382	arg1	foods					1384:1388	new foods	1380:1388	new foods	1380:1388	These differences were likely due to modification of the gut microbiota of the provisioned macaques to enable the digestion of new foods.
31880067	2	36	theme	animals	360:366	arg1	function					343:350	function	343:350	function	343:350	Captivity and human source food have been shown to influence drastically the gut microbiota composition and function of wild animals.
31880067	2	36	theme	animals	360:366	arg1	composition					327:337	gut microbiota composition	312:337	gut microbiota composition	312:337	Captivity and human source food have been shown to influence drastically the gut microbiota composition and function of wild animals.
31880067	0	37	theme	macaques	46:53	arg1	microbiota					4:13	Gut microbiota	0:13	Gut microbiota of provisioned and wild rhesus macaques (Macaca mulatta)	0:70	Gut microbiota of provisioned and wild rhesus macaques (Macaca mulatta) living in a limestone forest in southwest Guangxi, China.
31880067	6	38	theme	glycan	1018:1023	arg1	biosynthesis					1025:1036	glycan biosynthesis	1018:1036	glycan biosynthesis	1018:1036	Functional analysis revealed that wild macaques had enriched microbial pathways involved in glycan biosynthesis and metabolism, transport and catabolism, and the digestive and endocrine systems, while provisioned macaques were richer in pathways associated with signaling molecules and interaction, neurodegenerative diseases.
31880067	3	39	theme	present	387:393	arg1	study					395:399	the present study	383:399	the present study	383:399	Therefore, in the present study, the gut microbiota of provisioned and wild populations of limestone-living rhesus macaques (Macaca mulatta) were compared using high-throughput 16S rRNA sequencing and bioinformatic analyses.
31880067	3	40	theme	high-throughput	530:544	arg1	sequencing					555:564	high-throughput 16S rRNA sequencing	530:564	high-throughput 16S rRNA sequencing	530:564	Therefore, in the present study, the gut microbiota of provisioned and wild populations of limestone-living rhesus macaques (Macaca mulatta) were compared using high-throughput 16S rRNA sequencing and bioinformatic analyses.
31880067	6	41	theme	digestive	1088:1096	arg1	systems					1112:1118	the digestive and endocrine systems	1084:1118	the digestive and endocrine systems	1084:1118	Functional analysis revealed that wild macaques had enriched microbial pathways involved in glycan biosynthesis and metabolism, transport and catabolism, and the digestive and endocrine systems, while provisioned macaques were richer in pathways associated with signaling molecules and interaction, neurodegenerative diseases.
31880067	5	42	theme	lower	873:877	arg1	abundance					879:887	a lower abundance	871:887	a lower abundance of Bacteroidetes	871:904	Provisioned macaques also showed a higher abundance of Firmicutes and a lower abundance of Bacteroidetes than wild macaques.
31880067	0	43	theme	rhesus	39:44	arg1	mulatta					63:69	Macaca mulatta	56:69	Macaca mulatta	56:69	Gut microbiota of provisioned and wild rhesus macaques (Macaca mulatta) living in a limestone forest in southwest Guangxi, China.
31880067	0	43	theme	rhesus	39:44	arg1	macaques					46:53	provisioned and wild rhesus macaques	18:53	provisioned and wild rhesus macaques (Macaca mulatta)	18:70	Gut microbiota of provisioned and wild rhesus macaques (Macaca mulatta) living in a limestone forest in southwest Guangxi, China.
31880067	4	44	contain	had	642:644	arg1	macaques					633:640	provisioned macaques	621:640	provisioned macaques	621:640	The results indicated that provisioned macaques had a higher microbial richness than wild macaques, but there was no significant difference in the evenness of the gut microbiota between the two populations.
31880067	4	44	contain	had	642:644	arg2	richness					665:672	a higher microbial richness	646:672	a higher microbial richness	646:672	The results indicated that provisioned macaques had a higher microbial richness than wild macaques, but there was no significant difference in the evenness of the gut microbiota between the two populations.
31880067	3	45	theme	16S	546:548	arg1	sequencing					555:564	high-throughput 16S rRNA sequencing	530:564	high-throughput 16S rRNA sequencing	530:564	Therefore, in the present study, the gut microbiota of provisioned and wild populations of limestone-living rhesus macaques (Macaca mulatta) were compared using high-throughput 16S rRNA sequencing and bioinformatic analyses.
31880067	7	46	theme	macaques	1344:1351	arg1	microbiota					1314:1323	the gut microbiota	1306:1323	the gut microbiota of the provisioned macaques to enable the digestion of new foods	1306:1388	These differences were likely due to modification of the gut microbiota of the provisioned macaques to enable the digestion of new foods.
31880067	3	47	theme	wild	440:443	arg1	populations					445:455	provisioned and wild populations	424:455	provisioned and wild populations of limestone-living rhesus macaques (Macaca mulatta)	424:508	Therefore, in the present study, the gut microbiota of provisioned and wild populations of limestone-living rhesus macaques (Macaca mulatta) were compared using high-throughput 16S rRNA sequencing and bioinformatic analyses.
31880067	4	48	theme	gut	757:759	arg1	microbiota					761:770	the gut microbiota	753:770	the gut microbiota	753:770	The results indicated that provisioned macaques had a higher microbial richness than wild macaques, but there was no significant difference in the evenness of the gut microbiota between the two populations.
31880067	4	49	theme	higher	648:653	arg1	richness					665:672	a higher microbial richness	646:672	a higher microbial richness	646:672	The results indicated that provisioned macaques had a higher microbial richness than wild macaques, but there was no significant difference in the evenness of the gut microbiota between the two populations.
31880067	6	50	theme	endocrine	1102:1110	arg1	systems					1112:1118	the digestive and endocrine systems	1084:1118	the digestive and endocrine systems	1084:1118	Functional analysis revealed that wild macaques had enriched microbial pathways involved in glycan biosynthesis and metabolism, transport and catabolism, and the digestive and endocrine systems, while provisioned macaques were richer in pathways associated with signaling molecules and interaction, neurodegenerative diseases.
31880067	6	51	contain	had	974:976	arg2	pathways					997:1004	enriched microbial pathways	978:1004	enriched microbial pathways involved in glycan biosynthesis and metabolism, transport and catabolism, and the digestive and endocrine systems	978:1118	Functional analysis revealed that wild macaques had enriched microbial pathways involved in glycan biosynthesis and metabolism, transport and catabolism, and the digestive and endocrine systems, while provisioned macaques were richer in pathways associated with signaling molecules and interaction, neurodegenerative diseases.
31880067	6	51	contain	had	974:976	arg1	macaques					965:972	wild macaques	960:972	wild macaques	960:972	Functional analysis revealed that wild macaques had enriched microbial pathways involved in glycan biosynthesis and metabolism, transport and catabolism, and the digestive and endocrine systems, while provisioned macaques were richer in pathways associated with signaling molecules and interaction, neurodegenerative diseases.
31880067	3	52	theme	Macaca	494:499	arg1	macaques					484:491	limestone-living rhesus macaques	460:491	limestone-living rhesus macaques (Macaca mulatta)	460:508	Therefore, in the present study, the gut microbiota of provisioned and wild populations of limestone-living rhesus macaques (Macaca mulatta) were compared using high-throughput 16S rRNA sequencing and bioinformatic analyses.
31880067	3	52	theme	Macaca	494:499	arg1	mulatta					501:507	Macaca mulatta	494:507	Macaca mulatta	494:507	Therefore, in the present study, the gut microbiota of provisioned and wild populations of limestone-living rhesus macaques (Macaca mulatta) were compared using high-throughput 16S rRNA sequencing and bioinformatic analyses.
31880067	2	53	theme	microbiota	316:325	arg1	composition					327:337	gut microbiota composition	312:337	gut microbiota composition	312:337	Captivity and human source food have been shown to influence drastically the gut microbiota composition and function of wild animals.
31880067	0	54	theme	Macaca	56:61	arg1	mulatta					63:69	Macaca mulatta	56:69	Macaca mulatta	56:69	Gut microbiota of provisioned and wild rhesus macaques (Macaca mulatta) living in a limestone forest in southwest Guangxi, China.
31880067	0	54	theme	Macaca	56:61	arg1	macaques					46:53	provisioned and wild rhesus macaques	18:53	provisioned and wild rhesus macaques (Macaca mulatta)	18:70	Gut microbiota of provisioned and wild rhesus macaques (Macaca mulatta) living in a limestone forest in southwest Guangxi, China.
31880067	1	55	theme	animal	176:181	arg1	health					183:188	animal health	176:188	animal health	176:188	The gut microbiota plays an important role in animal health and is strongly affected by the environment.
31880067	4	56	theme	microbiota	761:770	arg1	evenness					741:748	the evenness	737:748	the evenness of the gut microbiota	737:770	The results indicated that provisioned macaques had a higher microbial richness than wild macaques, but there was no significant difference in the evenness of the gut microbiota between the two populations.
31880067	1	57	theme	important	158:166	arg1	role					168:171	an important role	155:171	an important role	155:171	The gut microbiota plays an important role in animal health and is strongly affected by the environment.
31880067	3	58	theme	limestone-living	460:475	arg1	macaques					484:491	limestone-living rhesus macaques	460:491	limestone-living rhesus macaques (Macaca mulatta)	460:508	Therefore, in the present study, the gut microbiota of provisioned and wild populations of limestone-living rhesus macaques (Macaca mulatta) were compared using high-throughput 16S rRNA sequencing and bioinformatic analyses.
31880067	3	58	theme	limestone-living	460:475	arg1	mulatta					501:507	Macaca mulatta	494:507	Macaca mulatta	494:507	Therefore, in the present study, the gut microbiota of provisioned and wild populations of limestone-living rhesus macaques (Macaca mulatta) were compared using high-throughput 16S rRNA sequencing and bioinformatic analyses.
31880067	7	59	theme	gut	1310:1312	arg1	microbiota					1314:1323	the gut microbiota	1306:1323	the gut microbiota of the provisioned macaques to enable the digestion of new foods	1306:1388	These differences were likely due to modification of the gut microbiota of the provisioned macaques to enable the digestion of new foods.
31880067	3	60	theme	bioinformatic	570:582	arg1	analyses					584:591	bioinformatic analyses	570:591	bioinformatic analyses	570:591	Therefore, in the present study, the gut microbiota of provisioned and wild populations of limestone-living rhesus macaques (Macaca mulatta) were compared using high-throughput 16S rRNA sequencing and bioinformatic analyses.
31880067	3	61	theme	rhesus	477:482	arg1	macaques					484:491	limestone-living rhesus macaques	460:491	limestone-living rhesus macaques (Macaca mulatta)	460:508	Therefore, in the present study, the gut microbiota of provisioned and wild populations of limestone-living rhesus macaques (Macaca mulatta) were compared using high-throughput 16S rRNA sequencing and bioinformatic analyses.
31880067	3	61	theme	rhesus	477:482	arg1	mulatta					501:507	Macaca mulatta	494:507	Macaca mulatta	494:507	Therefore, in the present study, the gut microbiota of provisioned and wild populations of limestone-living rhesus macaques (Macaca mulatta) were compared using high-throughput 16S rRNA sequencing and bioinformatic analyses.
31880067	0	62	from	forest	94:99	arg1	China					123:127	China	123:127	China	123:127	Gut microbiota of provisioned and wild rhesus macaques (Macaca mulatta) living in a limestone forest in southwest Guangxi, China.
29409818	0	0	theme	β-glucosidase	68:80	arg1	stability					53:61	stability	53:61	stability	53:61	Effect of N-linked glycosylation on the activity and stability of a β-glucosidase from Putranjiva roxburghii.
29409818	0	0	theme	β-glucosidase	68:80	arg1	activity					40:47	activity	40:47	activity	40:47	Effect of N-linked glycosylation on the activity and stability of a β-glucosidase from Putranjiva roxburghii.
29409818	2	1	gly	deglycosylation	287:301	arg1	PRGH1					306:310	PRGH1	306:310	PRGH1	306:310	The deglycosylation of PRGH1 was carried out by using PNGase F enzyme and confirmed by SDS-PAGE and carbohydrate estimation.
29409818	6	2	theme	glycosylated	1055:1066	arg1	form					1068:1071	the glycosylated form	1051:1071	the glycosylated form of PRGH1	1051:1080	DSC results showed deglycosylated form had lower Tm as compared to the glycosylated form of PRGH1.
29409818	2	3	theme	F	344:344	arg1	enzyme					346:351	PNGase F enzyme	337:351	PNGase F enzyme	337:351	The deglycosylation of PRGH1 was carried out by using PNGase F enzyme and confirmed by SDS-PAGE and carbohydrate estimation.
29409818	6	4	dep	showed	996:1001	arg1	had					1023:1025	had	1023:1025	showed deglycosylated form had lower Tm as compared to the glycosylated form of PRGH1	996:1080	DSC results showed deglycosylated form had lower Tm as compared to the glycosylated form of PRGH1.
29409818	5	5	gly	glycosylation	864:876	arg1	protein					928:934	protein	928:934	protein	928:934	Circular dichroism, fluorescence studies and differential scanning calorimetry (DSC) analysis demonstrated the possible effect of glycosylation on local and/or global conformational dynamics of protein and its effect on the thermostability of PRGH1.
29409818	5	6	from	dynamics	916:923	arg1	thermostability					958:972	the thermostability	954:972	the thermostability of PRGH1	954:981	Circular dichroism, fluorescence studies and differential scanning calorimetry (DSC) analysis demonstrated the possible effect of glycosylation on local and/or global conformational dynamics of protein and its effect on the thermostability of PRGH1.
29409818	1	7	theme	N-glycosylation	139:153	arg1	effect					129:134	the effect	125:134	the effect of N-glycosylation on the conformational and functional stability of Putranjiva roxburghii family 1 β-glucosidase (PRGH1) enzyme	125:263	In this study, the effect of N-glycosylation on the conformational and functional stability of Putranjiva roxburghii family 1 β-glucosidase (PRGH1) enzyme was investigated.
29409818	4	8	from	reduction	679:687	arg1	Vmax					692:695	Vmax	692:695	Vmax	692:695	The deglycosylation of PRGH1 affected enzyme activity to a certain extent only where Km was not affected but a slight reduction in Vmax for various substrates was observed.
29409818	1	9	theme	PRGH1	251:255	arg1	enzyme					258:263	Putranjiva roxburghii family 1 β-glucosidase (PRGH1) enzyme	205:263	Putranjiva roxburghii family 1 β-glucosidase (PRGH1) enzyme	205:263	In this study, the effect of N-glycosylation on the conformational and functional stability of Putranjiva roxburghii family 1 β-glucosidase (PRGH1) enzyme was investigated.
29409818	0	10	from	Effect	0:5	arg1	stability					53:61	stability	53:61	stability	53:61	Effect of N-linked glycosylation on the activity and stability of a β-glucosidase from Putranjiva roxburghii.
29409818	0	10	from	Effect	0:5	arg1	activity					40:47	activity	40:47	activity	40:47	Effect of N-linked glycosylation on the activity and stability of a β-glucosidase from Putranjiva roxburghii.
29409818	0	11	link	N-linked	10:17	arg1	glycosylation					19:31	N-linked glycosylation	10:31	N-linked glycosylation	10:31	Effect of N-linked glycosylation on the activity and stability of a β-glucosidase from Putranjiva roxburghii.
29409818	3	12	theme	deglycosylated	539:552	arg1	PRGH1					554:558	deglycosylated PRGH1	539:558	deglycosylated PRGH1	539:558	Comparative analysis with respect to enzyme activity, stability and aggregation behaviour was carried out for the glycosylated and deglycosylated PRGH1.
29409818	6	13	theme	deglycosylated	1003:1016	arg1	form					1018:1021	deglycosylated form	1003:1021	deglycosylated form	1003:1021	DSC results showed deglycosylated form had lower Tm as compared to the glycosylated form of PRGH1.
29409818	1	14	theme	enzyme	258:263	arg1	stability					192:200	the conformational and functional stability	158:200	the conformational and functional stability of Putranjiva roxburghii family 1 β-glucosidase (PRGH1) enzyme	158:263	In this study, the effect of N-glycosylation on the conformational and functional stability of Putranjiva roxburghii family 1 β-glucosidase (PRGH1) enzyme was investigated.
29409818	5	15	theme	Circular	734:741	arg1	dichroism					743:751	Circular dichroism	734:751	Circular dichroism	734:751	Circular dichroism, fluorescence studies and differential scanning calorimetry (DSC) analysis demonstrated the possible effect of glycosylation on local and/or global conformational dynamics of protein and its effect on the thermostability of PRGH1.
29409818	0	16	from	activity	40:47	arg1	Putranjiva					87:96	Putranjiva	87:96	Putranjiva	87:96	Effect of N-linked glycosylation on the activity and stability of a β-glucosidase from Putranjiva roxburghii.
29409818	1	17	from	effect	129:134	arg1	stability					192:200	the conformational and functional stability	158:200	the conformational and functional stability of Putranjiva roxburghii family 1 β-glucosidase (PRGH1) enzyme	158:263	In this study, the effect of N-glycosylation on the conformational and functional stability of Putranjiva roxburghii family 1 β-glucosidase (PRGH1) enzyme was investigated.
29409818	5	18	theme	local	881:885	arg1	dynamics					916:923	local and/or global conformational dynamics	881:923	local and/or global conformational dynamics of protein	881:934	Circular dichroism, fluorescence studies and differential scanning calorimetry (DSC) analysis demonstrated the possible effect of glycosylation on local and/or global conformational dynamics of protein and its effect on the thermostability of PRGH1.
29409818	5	19	theme	scanning	792:799	arg1	calorimetry					801:811	differential scanning calorimetry	779:811	differential scanning calorimetry (DSC)	779:817	Circular dichroism, fluorescence studies and differential scanning calorimetry (DSC) analysis demonstrated the possible effect of glycosylation on local and/or global conformational dynamics of protein and its effect on the thermostability of PRGH1.
29409818	5	19	theme	scanning	792:799	arg1	DSC					814:816	DSC	814:816	DSC	814:816	Circular dichroism, fluorescence studies and differential scanning calorimetry (DSC) analysis demonstrated the possible effect of glycosylation on local and/or global conformational dynamics of protein and its effect on the thermostability of PRGH1.
29409818	4	20	theme	enzyme	599:604	arg1	activity					606:613	enzyme activity	599:613	enzyme activity	599:613	The deglycosylation of PRGH1 affected enzyme activity to a certain extent only where Km was not affected but a slight reduction in Vmax for various substrates was observed.
29409818	9	21	theme	PRGH1	1455:1459	arg1	stability					1442:1450	conformational stability	1427:1450	conformational stability of PRGH1	1427:1459	The results demonstrated that N-linked glycosylation played an important role in conformational stability of PRGH1; however, it did not affect the enzyme function drastically.
29409818	7	22	theme	glycosylated	1181:1192	arg1	compact					1210:1216	compact	1210:1216	compact	1210:1216	The PRGH1 was found to be more sensitive to proteolysis after deglycosylation suggesting that the glycosylated PRGH1 was quite compact and rigid.
29409818	7	22	theme	glycosylated	1181:1192	arg1	PRGH1					1194:1198	the glycosylated PRGH1	1177:1198	the glycosylated PRGH1	1177:1198	The PRGH1 was found to be more sensitive to proteolysis after deglycosylation suggesting that the glycosylated PRGH1 was quite compact and rigid.
29409818	9	23	theme	N-linked	1376:1383	arg1	glycosylation					1385:1397	N-linked glycosylation	1376:1397	N-linked glycosylation	1376:1397	The results demonstrated that N-linked glycosylation played an important role in conformational stability of PRGH1; however, it did not affect the enzyme function drastically.
29409818	5	24	from	effect	944:949	arg1	thermostability					958:972	the thermostability	954:972	the thermostability of PRGH1	954:981	Circular dichroism, fluorescence studies and differential scanning calorimetry (DSC) analysis demonstrated the possible effect of glycosylation on local and/or global conformational dynamics of protein and its effect on the thermostability of PRGH1.
29409818	6	25	theme	PRGH1	1076:1080	arg1	form					1068:1071	the glycosylated form	1051:1071	the glycosylated form of PRGH1	1051:1080	DSC results showed deglycosylated form had lower Tm as compared to the glycosylated form of PRGH1.
29409818	1	26	theme	conformational	162:175	arg1	stability					192:200	the conformational and functional stability	158:200	the conformational and functional stability of Putranjiva roxburghii family 1 β-glucosidase (PRGH1) enzyme	158:263	In this study, the effect of N-glycosylation on the conformational and functional stability of Putranjiva roxburghii family 1 β-glucosidase (PRGH1) enzyme was investigated.
29409818	8	27	gly	glycosylation	1295:1307	arg2	sites					1309:1313	out of seven potential N-linked glycosylation sites	1263:1313	out of seven potential N-linked glycosylation sites	1263:1313	Mutagenesis studies revealed that out of seven potential N-linked glycosylation sites, only three were glycosylated.
29409818	8	27	gly	glycosylation	1295:1307	arg2	three					1321:1325	three	1321:1325	three	1321:1325	Mutagenesis studies revealed that out of seven potential N-linked glycosylation sites, only three were glycosylated.
29409818	8	27	gly	glycosylation	1295:1307	arg2	seven					1270:1274	seven	1270:1274	seven	1270:1274	Mutagenesis studies revealed that out of seven potential N-linked glycosylation sites, only three were glycosylated.
29409818	5	28	theme	glycosylation	864:876	arg1	effect					854:859	the possible effect	841:859	the possible effect of glycosylation on local and/or global conformational dynamics of protein and its effect on the thermostability of PRGH1	841:981	Circular dichroism, fluorescence studies and differential scanning calorimetry (DSC) analysis demonstrated the possible effect of glycosylation on local and/or global conformational dynamics of protein and its effect on the thermostability of PRGH1.
29409818	2	29	theme	PRGH1	306:310	arg1	deglycosylation					287:301	The deglycosylation	283:301	The deglycosylation of PRGH1	283:310	The deglycosylation of PRGH1 was carried out by using PNGase F enzyme and confirmed by SDS-PAGE and carbohydrate estimation.
29409818	0	30	theme	glycosylation	19:31	arg1	Effect					0:5	Effect	0:5	Effect of N-linked glycosylation on the activity and stability of a β-glucosidase from Putranjiva roxburghii.	0:108	Effect of N-linked glycosylation on the activity and stability of a β-glucosidase from Putranjiva roxburghii.
29409818	6	31	theme	DSC	984:986	arg1	results					988:994	DSC results	984:994	DSC results	984:994	DSC results showed deglycosylated form had lower Tm as compared to the glycosylated form of PRGH1.
29409818	6	32	gly	glycosylated	1055:1066	arg1	PRGH1					1076:1080	PRGH1	1076:1080	PRGH1	1076:1080	DSC results showed deglycosylated form had lower Tm as compared to the glycosylated form of PRGH1.
29409818	6	32	gly	glycosylated	1055:1066	arg1	form					1068:1071	the glycosylated form	1051:1071	the glycosylated form of PRGH1	1051:1080	DSC results showed deglycosylated form had lower Tm as compared to the glycosylated form of PRGH1.
29409818	5	33	theme	protein	928:934	arg1	dynamics					916:923	local and/or global conformational dynamics	881:923	local and/or global conformational dynamics of protein	881:934	Circular dichroism, fluorescence studies and differential scanning calorimetry (DSC) analysis demonstrated the possible effect of glycosylation on local and/or global conformational dynamics of protein and its effect on the thermostability of PRGH1.
29409818	5	33	theme	protein	928:934	arg1	effect					944:949	its effect	940:949	its effect on the thermostability of PRGH1	940:981	Circular dichroism, fluorescence studies and differential scanning calorimetry (DSC) analysis demonstrated the possible effect of glycosylation on local and/or global conformational dynamics of protein and its effect on the thermostability of PRGH1.
29409818	6	34	theme	lower	1027:1031	arg1	Tm					1033:1034	lower Tm	1027:1034	lower Tm	1027:1034	DSC results showed deglycosylated form had lower Tm as compared to the glycosylated form of PRGH1.
29409818	1	35	theme	functional	181:190	arg1	stability					192:200	the conformational and functional stability	158:200	the conformational and functional stability of Putranjiva roxburghii family 1 β-glucosidase (PRGH1) enzyme	158:263	In this study, the effect of N-glycosylation on the conformational and functional stability of Putranjiva roxburghii family 1 β-glucosidase (PRGH1) enzyme was investigated.
29409818	0	36	theme	N-linked	10:17	arg1	glycosylation					19:31	N-linked glycosylation	10:31	N-linked glycosylation	10:31	Effect of N-linked glycosylation on the activity and stability of a β-glucosidase from Putranjiva roxburghii.
29409818	2	37	theme	PNGase	337:342	arg1	F					344:344	PNGase F	337:344	PNGase F enzyme	337:351	The deglycosylation of PRGH1 was carried out by using PNGase F enzyme and confirmed by SDS-PAGE and carbohydrate estimation.
29409818	5	38	theme	possible	845:852	arg1	effect					854:859	the possible effect	841:859	the possible effect of glycosylation on local and/or global conformational dynamics of protein and its effect on the thermostability of PRGH1	841:981	Circular dichroism, fluorescence studies and differential scanning calorimetry (DSC) analysis demonstrated the possible effect of glycosylation on local and/or global conformational dynamics of protein and its effect on the thermostability of PRGH1.
29409818	5	39	theme	differential	779:790	arg1	calorimetry					801:811	differential scanning calorimetry	779:811	differential scanning calorimetry (DSC)	779:817	Circular dichroism, fluorescence studies and differential scanning calorimetry (DSC) analysis demonstrated the possible effect of glycosylation on local and/or global conformational dynamics of protein and its effect on the thermostability of PRGH1.
29409818	5	39	theme	differential	779:790	arg1	DSC					814:816	DSC	814:816	DSC	814:816	Circular dichroism, fluorescence studies and differential scanning calorimetry (DSC) analysis demonstrated the possible effect of glycosylation on local and/or global conformational dynamics of protein and its effect on the thermostability of PRGH1.
29409818	5	40	theme	PRGH1	977:981	arg1	thermostability					958:972	the thermostability	954:972	the thermostability of PRGH1	954:981	Circular dichroism, fluorescence studies and differential scanning calorimetry (DSC) analysis demonstrated the possible effect of glycosylation on local and/or global conformational dynamics of protein and its effect on the thermostability of PRGH1.
29409818	8	41	theme	glycosylation	1295:1307	arg1	sites					1309:1313	out of seven potential N-linked glycosylation sites	1263:1313	out of seven potential N-linked glycosylation sites	1263:1313	Mutagenesis studies revealed that out of seven potential N-linked glycosylation sites, only three were glycosylated.
29409818	7	42	gly	glycosylated	1181:1192	arg1	compact					1210:1216	compact	1210:1216	compact	1210:1216	The PRGH1 was found to be more sensitive to proteolysis after deglycosylation suggesting that the glycosylated PRGH1 was quite compact and rigid.
29409818	7	42	gly	glycosylated	1181:1192	arg1	PRGH1					1194:1198	the glycosylated PRGH1	1177:1198	the glycosylated PRGH1	1177:1198	The PRGH1 was found to be more sensitive to proteolysis after deglycosylation suggesting that the glycosylated PRGH1 was quite compact and rigid.
29409818	3	43	theme	enzyme	445:450	arg1	activity					452:459	enzyme activity	445:459	enzyme activity	445:459	Comparative analysis with respect to enzyme activity, stability and aggregation behaviour was carried out for the glycosylated and deglycosylated PRGH1.
29409818	3	44	theme	Comparative	408:418	arg1	analysis					420:427	Comparative analysis	408:427	Comparative analysis with respect to enzyme activity, stability and aggregation behaviour	408:496	Comparative analysis with respect to enzyme activity, stability and aggregation behaviour was carried out for the glycosylated and deglycosylated PRGH1.
29409818	4	45	theme	PRGH1	584:588	arg1	deglycosylation					565:579	The deglycosylation	561:579	The deglycosylation of PRGH1	561:588	The deglycosylation of PRGH1 affected enzyme activity to a certain extent only where Km was not affected but a slight reduction in Vmax for various substrates was observed.
29409818	0	46	dep	activity	40:47	arg1	the					36:38	the	36:38	the	36:38	Effect of N-linked glycosylation on the activity and stability of a β-glucosidase from Putranjiva roxburghii.
29409818	5	47	from	effect	854:859	arg1	dynamics					916:923	local and/or global conformational dynamics	881:923	local and/or global conformational dynamics of protein	881:934	Circular dichroism, fluorescence studies and differential scanning calorimetry (DSC) analysis demonstrated the possible effect of glycosylation on local and/or global conformational dynamics of protein and its effect on the thermostability of PRGH1.
29409818	5	47	from	effect	854:859	arg1	effect					944:949	its effect	940:949	its effect on the thermostability of PRGH1	940:981	Circular dichroism, fluorescence studies and differential scanning calorimetry (DSC) analysis demonstrated the possible effect of glycosylation on local and/or global conformational dynamics of protein and its effect on the thermostability of PRGH1.
29409818	1	48	theme	Putranjiva	205:214	arg1	enzyme					258:263	Putranjiva roxburghii family 1 β-glucosidase (PRGH1) enzyme	205:263	Putranjiva roxburghii family 1 β-glucosidase (PRGH1) enzyme	205:263	In this study, the effect of N-glycosylation on the conformational and functional stability of Putranjiva roxburghii family 1 β-glucosidase (PRGH1) enzyme was investigated.
29409818	9	49	link	N-linked	1376:1383	arg1	glycosylation					1385:1397	N-linked glycosylation	1376:1397	N-linked glycosylation	1376:1397	The results demonstrated that N-linked glycosylation played an important role in conformational stability of PRGH1; however, it did not affect the enzyme function drastically.
29409818	0	50	from	stability	53:61	arg1	Putranjiva					87:96	Putranjiva	87:96	Putranjiva	87:96	Effect of N-linked glycosylation on the activity and stability of a β-glucosidase from Putranjiva roxburghii.
29409818	9	51	theme	enzyme	1493:1498	arg1	function					1500:1507	the enzyme function	1489:1507	the enzyme function	1489:1507	The results demonstrated that N-linked glycosylation played an important role in conformational stability of PRGH1; however, it did not affect the enzyme function drastically.
29409818	4	52	theme	certain	620:626	arg1	extent					628:633	a certain extent	618:633	a certain extent only where Km was not affected but a slight reduction in Vmax for various substrates was observed	618:731	The deglycosylation of PRGH1 affected enzyme activity to a certain extent only where Km was not affected but a slight reduction in Vmax for various substrates was observed.
29409818	4	53	theme	various	701:707	arg1	substrates					709:718	various substrates	701:718	various substrates	701:718	The deglycosylation of PRGH1 affected enzyme activity to a certain extent only where Km was not affected but a slight reduction in Vmax for various substrates was observed.
29409818	8	54	theme	Mutagenesis	1229:1239	arg1	studies					1241:1247	Mutagenesis studies	1229:1247	Mutagenesis studies	1229:1247	Mutagenesis studies revealed that out of seven potential N-linked glycosylation sites, only three were glycosylated.
29409818	5	55	dep	dichroism	743:751	arg1	analysis					819:826	analysis	819:826	analysis	819:826	Circular dichroism, fluorescence studies and differential scanning calorimetry (DSC) analysis demonstrated the possible effect of glycosylation on local and/or global conformational dynamics of protein and its effect on the thermostability of PRGH1.
29409818	0	56	gly	glycosylation	19:31	arg1	β-glucosidase					68:80	a β-glucosidase	66:80	a β-glucosidase from Putranjiva roxburghii	66:107	Effect of N-linked glycosylation on the activity and stability of a β-glucosidase from Putranjiva roxburghii.
29409818	4	57	gly	deglycosylation	565:579	arg1	enzyme					599:604	enzyme activity	599:613	enzyme activity	599:613	The deglycosylation of PRGH1 affected enzyme activity to a certain extent only where Km was not affected but a slight reduction in Vmax for various substrates was observed.
29409818	4	57	gly	deglycosylation	565:579	arg1	PRGH1					584:588	PRGH1	584:588	PRGH1	584:588	The deglycosylation of PRGH1 affected enzyme activity to a certain extent only where Km was not affected but a slight reduction in Vmax for various substrates was observed.
29409818	9	58	theme	important	1409:1417	arg1	role					1419:1422	an important role	1406:1422	an important role	1406:1422	The results demonstrated that N-linked glycosylation played an important role in conformational stability of PRGH1; however, it did not affect the enzyme function drastically.
29409818	1	59	gly	N-glycosylation	139:153	arg1	enzyme					258:263	Putranjiva roxburghii family 1 β-glucosidase (PRGH1) enzyme	205:263	Putranjiva roxburghii family 1 β-glucosidase (PRGH1) enzyme	205:263	In this study, the effect of N-glycosylation on the conformational and functional stability of Putranjiva roxburghii family 1 β-glucosidase (PRGH1) enzyme was investigated.
29409818	8	60	link	N-linked	1286:1293	arg1	sites					1309:1313	out of seven potential N-linked glycosylation sites	1263:1313	out of seven potential N-linked glycosylation sites	1263:1313	Mutagenesis studies revealed that out of seven potential N-linked glycosylation sites, only three were glycosylated.
29409818	4	61	theme	slight	672:677	arg1	reduction					679:687	a slight reduction	670:687	a slight reduction in Vmax for various substrates	670:718	The deglycosylation of PRGH1 affected enzyme activity to a certain extent only where Km was not affected but a slight reduction in Vmax for various substrates was observed.
29409818	8	62	theme	N-linked	1286:1293	arg1	sites					1309:1313	out of seven potential N-linked glycosylation sites	1263:1313	out of seven potential N-linked glycosylation sites	1263:1313	Mutagenesis studies revealed that out of seven potential N-linked glycosylation sites, only three were glycosylated.
29409818	6	63	gly	deglycosylated	1003:1016	arg1	form					1018:1021	deglycosylated form	1003:1021	deglycosylated form	1003:1021	DSC results showed deglycosylated form had lower Tm as compared to the glycosylated form of PRGH1.
29409818	0	64	from	Putranjiva	87:96	arg1	stability					53:61	stability	53:61	stability	53:61	Effect of N-linked glycosylation on the activity and stability of a β-glucosidase from Putranjiva roxburghii.
29409818	0	64	from	Putranjiva	87:96	arg1	β-glucosidase					68:80	a β-glucosidase	66:80	a β-glucosidase from Putranjiva roxburghii	66:107	Effect of N-linked glycosylation on the activity and stability of a β-glucosidase from Putranjiva roxburghii.
29409818	0	64	from	Putranjiva	87:96	arg1	activity					40:47	activity	40:47	activity	40:47	Effect of N-linked glycosylation on the activity and stability of a β-glucosidase from Putranjiva roxburghii.
29409818	1	65	dep	Putranjiva	205:214	arg1	roxburghii					216:225	roxburghii	216:225	roxburghii	216:225	In this study, the effect of N-glycosylation on the conformational and functional stability of Putranjiva roxburghii family 1 β-glucosidase (PRGH1) enzyme was investigated.
29409818	9	66	theme	conformational	1427:1440	arg1	stability					1442:1450	conformational stability	1427:1450	conformational stability of PRGH1	1427:1459	The results demonstrated that N-linked glycosylation played an important role in conformational stability of PRGH1; however, it did not affect the enzyme function drastically.
29409818	3	67	theme	aggregation	476:486	arg1	behaviour					488:496	aggregation behaviour	476:496	aggregation behaviour	476:496	Comparative analysis with respect to enzyme activity, stability and aggregation behaviour was carried out for the glycosylated and deglycosylated PRGH1.
29409818	1	68	theme	family	227:232	arg1	enzyme					258:263	Putranjiva roxburghii family 1 β-glucosidase (PRGH1) enzyme	205:263	Putranjiva roxburghii family 1 β-glucosidase (PRGH1) enzyme	205:263	In this study, the effect of N-glycosylation on the conformational and functional stability of Putranjiva roxburghii family 1 β-glucosidase (PRGH1) enzyme was investigated.
29409818	5	69	theme	conformational	901:914	arg1	dynamics					916:923	local and/or global conformational dynamics	881:923	local and/or global conformational dynamics of protein	881:934	Circular dichroism, fluorescence studies and differential scanning calorimetry (DSC) analysis demonstrated the possible effect of glycosylation on local and/or global conformational dynamics of protein and its effect on the thermostability of PRGH1.
29409818	8	70	theme	potential	1276:1284	arg1	sites					1309:1313	out of seven potential N-linked glycosylation sites	1263:1313	out of seven potential N-linked glycosylation sites	1263:1313	Mutagenesis studies revealed that out of seven potential N-linked glycosylation sites, only three were glycosylated.
29409818	5	71	theme	global	894:899	arg1	dynamics					916:923	local and/or global conformational dynamics	881:923	local and/or global conformational dynamics of protein	881:934	Circular dichroism, fluorescence studies and differential scanning calorimetry (DSC) analysis demonstrated the possible effect of glycosylation on local and/or global conformational dynamics of protein and its effect on the thermostability of PRGH1.
29409818	8	72	gly	glycosylated	1332:1343	arg1	sites					1309:1313	out of seven potential N-linked glycosylation sites	1263:1313	out of seven potential N-linked glycosylation sites	1263:1313	Mutagenesis studies revealed that out of seven potential N-linked glycosylation sites, only three were glycosylated.
29409818	5	73	theme	fluorescence	754:765	arg1	studies					767:773	fluorescence studies	754:773	fluorescence studies	754:773	Circular dichroism, fluorescence studies and differential scanning calorimetry (DSC) analysis demonstrated the possible effect of glycosylation on local and/or global conformational dynamics of protein and its effect on the thermostability of PRGH1.
29409818	2	74	dep	SDS-PAGE	370:377	arg1	estimation					396:405	estimation	396:405	estimation	396:405	The deglycosylation of PRGH1 was carried out by using PNGase F enzyme and confirmed by SDS-PAGE and carbohydrate estimation.
29409818	3	75	gly	deglycosylated	539:552	arg1	PRGH1					554:558	deglycosylated PRGH1	539:558	deglycosylated PRGH1	539:558	Comparative analysis with respect to enzyme activity, stability and aggregation behaviour was carried out for the glycosylated and deglycosylated PRGH1.
29409818	1	76	theme	β-glucosidase	236:248	arg1	enzyme					258:263	Putranjiva roxburghii family 1 β-glucosidase (PRGH1) enzyme	205:263	Putranjiva roxburghii family 1 β-glucosidase (PRGH1) enzyme	205:263	In this study, the effect of N-glycosylation on the conformational and functional stability of Putranjiva roxburghii family 1 β-glucosidase (PRGH1) enzyme was investigated.
31754200	9	0	theme	narrow	1132:1137	arg1	variation					1148:1156	Remarkably narrow sequence variation	1121:1156	Remarkably narrow sequence variation	1121:1156	Remarkably narrow sequence variation was found within the HvCslF6 promoter and coding sequence and does not explain variation in (1,3;1,4)-β-glucan content.
31754200	10	1	dep	1,3	1337:1339	arg1	1,4					1341:1343	1,4	1341:1343	1,3;1,4	1337:1343	Our data emphasise the genotype-dependent accumulation of (1,3;1,4)-β-glucan during barley grain development and a role for the balance between hydrolysis and synthesis in determining (1,3;1,4)-β-glucan content, and suggests that other regulatory sequences or proteins are likely to be involved in this trait in developing grain.
31754200	5	2	dep	1,3	669:671	arg1	1,4					673:675	1,4	673:675	1,3;1,4	669:675	Here, the relationship between mRNA levels of HvCslF6, HvCslF9, HvGlbI (1,3;1,4)-β-glucan endohydrolase, and (1,3;1,4)-β-glucan content was studied in developing grains of four barley cultivars.
31754200	7	3	theme	HvGlbI	930:935	arg1	expression					937:946	HvGlbI expression	930:946	HvGlbI expression	930:946	A peak of HvGlbI expression was detected at 15 DPA.
31754200	10	4	theme	regulatory	1514:1523	arg1	sequences					1525:1533	other regulatory sequences	1508:1533	other regulatory sequences	1508:1533	Our data emphasise the genotype-dependent accumulation of (1,3;1,4)-β-glucan during barley grain development and a role for the balance between hydrolysis and synthesis in determining (1,3;1,4)-β-glucan content, and suggests that other regulatory sequences or proteins are likely to be involved in this trait in developing grain.
31754200	8	5	theme	transcript	987:996	arg1	abundance					998:1006	transcript abundance	987:1006	transcript abundance	987:1006	Differences in transcript abundance across the three genes could partially explain variation in grain (1,3;1,4)-β-glucan content in these genotypes.
31754200	5	6	theme	developing	710:719	arg1	grains					721:726	developing grains	710:726	developing grains of four barley cultivars	710:751	Here, the relationship between mRNA levels of HvCslF6, HvCslF9, HvGlbI (1,3;1,4)-β-glucan endohydrolase, and (1,3;1,4)-β-glucan content was studied in developing grains of four barley cultivars.
31754200	4	7	theme	HvCslF9	539:545	arg1	unknown					550:556	unknown	550:556	unknown	550:556	HvCslF6 encodes a grain (1,3;1,4)-β-glucan synthase, whereas the function of HvCslF9 is unknown.
31754200	4	7	theme	HvCslF9	539:545	arg1	function					527:534	the function	523:534	the function of HvCslF9	523:545	HvCslF6 encodes a grain (1,3;1,4)-β-glucan synthase, whereas the function of HvCslF9 is unknown.
31754200	10	8	theme	grain	1369:1373	arg1	development					1375:1385	barley grain development	1362:1385	barley grain development	1362:1385	Our data emphasise the genotype-dependent accumulation of (1,3;1,4)-β-glucan during barley grain development and a role for the balance between hydrolysis and synthesis in determining (1,3;1,4)-β-glucan content, and suggests that other regulatory sequences or proteins are likely to be involved in this trait in developing grain.
31754200	5	9	theme	HvCslF6	605:611	arg1	levels					595:600	mRNA levels	590:600	mRNA levels of HvCslF6, HvCslF9, HvGlbI (1,3;1,4)-β-glucan endohydrolase, and (1,3;1,4)-β-glucan content	590:693	Here, the relationship between mRNA levels of HvCslF6, HvCslF9, HvGlbI (1,3;1,4)-β-glucan endohydrolase, and (1,3;1,4)-β-glucan content was studied in developing grains of four barley cultivars.
31754200	2	10	theme	cereal	268:273	arg1	grains					275:280	other widely consumed cereal grains	246:280	other widely consumed cereal grains	246:280	Unlike other widely consumed cereal grains barley is comparatively rich in (1,3;1,4)-β-glucan, a source of dietary fibre.
31754200	5	11	theme	content	687:693	arg1	levels					595:600	mRNA levels	590:600	mRNA levels of HvCslF6, HvCslF9, HvGlbI (1,3;1,4)-β-glucan endohydrolase, and (1,3;1,4)-β-glucan content	590:693	Here, the relationship between mRNA levels of HvCslF6, HvCslF9, HvGlbI (1,3;1,4)-β-glucan endohydrolase, and (1,3;1,4)-β-glucan content was studied in developing grains of four barley cultivars.
31754200	0	12	from	effects	41:47	arg1	genes					89:93	genes	89:93	genes encoding the corresponding synthase and endohydrolase enzymes	89:155	Barley grain (1,3;1,4)-β-glucan content: effects of transcript and sequence variation in genes encoding the corresponding synthase and endohydrolase enzymes.
31754200	1	13	theme	walls	188:192	arg1	composition					162:172	The composition	158:172	The composition of plant cell walls	158:192	The composition of plant cell walls is important in determining cereal end uses.
31754200	1	13	theme	walls	188:192	arg1	important					197:205	important	197:205	important	197:205	The composition of plant cell walls is important in determining cereal end uses.
31754200	5	14	theme	mRNA	590:593	arg1	levels					595:600	mRNA levels	590:600	mRNA levels of HvCslF6, HvCslF9, HvGlbI (1,3;1,4)-β-glucan endohydrolase, and (1,3;1,4)-β-glucan content	590:693	Here, the relationship between mRNA levels of HvCslF6, HvCslF9, HvGlbI (1,3;1,4)-β-glucan endohydrolase, and (1,3;1,4)-β-glucan content was studied in developing grains of four barley cultivars.
31754200	0	15	theme	corresponding	108:120	arg1	synthase					122:129	corresponding synthase	108:129	corresponding synthase	108:129	Barley grain (1,3;1,4)-β-glucan content: effects of transcript and sequence variation in genes encoding the corresponding synthase and endohydrolase enzymes.
31754200	10	16	theme	other	1508:1512	arg1	sequences					1525:1533	other regulatory sequences	1508:1533	other regulatory sequences	1508:1533	Our data emphasise the genotype-dependent accumulation of (1,3;1,4)-β-glucan during barley grain development and a role for the balance between hydrolysis and synthesis in determining (1,3;1,4)-β-glucan content, and suggests that other regulatory sequences or proteins are likely to be involved in this trait in developing grain.
31754200	1	17	dep	end	229:231	arg1	uses					233:236	uses	233:236	uses	233:236	The composition of plant cell walls is important in determining cereal end uses.
31754200	5	18	theme	barley	736:741	arg1	cultivars					743:751	four barley cultivars	731:751	four barley cultivars	731:751	Here, the relationship between mRNA levels of HvCslF6, HvCslF9, HvGlbI (1,3;1,4)-β-glucan endohydrolase, and (1,3;1,4)-β-glucan content was studied in developing grains of four barley cultivars.
31754200	6	19	theme	grain	831:835	arg1	stages					849:854	grain development stages	831:854	grain development stages	831:854	HvCslF6 was differentially expressed during mid (8-15 DPA) and late (38 DPA) grain development stages while HvCslF9 transcript was only clearly detected at 8-10 DPA.
31754200	5	20	theme	-β-glucan	639:647	arg1	endohydrolase					649:661	HvCslF9, HvGlbI (1,3;1,4)-β-glucan endohydrolase	614:661	HvCslF9, HvGlbI (1,3;1,4)-β-glucan endohydrolase	614:661	Here, the relationship between mRNA levels of HvCslF6, HvCslF9, HvGlbI (1,3;1,4)-β-glucan endohydrolase, and (1,3;1,4)-β-glucan content was studied in developing grains of four barley cultivars.
31754200	0	21	theme	transcript	52:61	arg1	effects					41:47	effects	41:47	Barley grain (1,3;1,4)-β-glucan content: effects of transcript and sequence variation in genes encoding the corresponding synthase and endohydrolase enzymes.	0:156	Barley grain (1,3;1,4)-β-glucan content: effects of transcript and sequence variation in genes encoding the corresponding synthase and endohydrolase enzymes.
31754200	8	22	from	content	1093:1099	arg1	genotypes					1110:1118	these genotypes	1104:1118	these genotypes	1104:1118	Differences in transcript abundance across the three genes could partially explain variation in grain (1,3;1,4)-β-glucan content in these genotypes.
31754200	0	23	theme	sequence	67:74	arg1	variation					76:84	sequence variation	67:84	sequence variation	67:84	Barley grain (1,3;1,4)-β-glucan content: effects of transcript and sequence variation in genes encoding the corresponding synthase and endohydrolase enzymes.
31754200	8	24	dep	1,3	1075:1077	arg1	1,4					1079:1081	1,4	1079:1081	1,3;1,4	1075:1081	Differences in transcript abundance across the three genes could partially explain variation in grain (1,3;1,4)-β-glucan content in these genotypes.
31754200	6	25	located	detected	898:905	arg1	DPA					915:917	8-10 DPA	910:917	8-10 DPA	910:917	HvCslF6 was differentially expressed during mid (8-15 DPA) and late (38 DPA) grain development stages while HvCslF9 transcript was only clearly detected at 8-10 DPA.
31754200	6	25	located	detected	898:905	arg2	transcript					870:879	HvCslF9 transcript	862:879	HvCslF9 transcript	862:879	HvCslF6 was differentially expressed during mid (8-15 DPA) and late (38 DPA) grain development stages while HvCslF9 transcript was only clearly detected at 8-10 DPA.
31754200	3	26	theme	Previous	361:368	arg1	work					370:373	Previous work	361:373	Previous work	361:373	Previous work showed Cellulose synthase-like genes synthesise (1,3;1,4)-β-glucan in several tissues.
31754200	0	27	theme	Barley	0:5	arg1	grain					7:11	Barley grain	0:11	Barley grain (1,3;1,4)-β-glucan content: effects of transcript and sequence variation in genes encoding the corresponding synthase and endohydrolase enzymes.	0:156	Barley grain (1,3;1,4)-β-glucan content: effects of transcript and sequence variation in genes encoding the corresponding synthase and endohydrolase enzymes.
31754200	9	28	theme	sequence	1139:1146	arg1	variation					1148:1156	Remarkably narrow sequence variation	1121:1156	Remarkably narrow sequence variation	1121:1156	Remarkably narrow sequence variation was found within the HvCslF6 promoter and coding sequence and does not explain variation in (1,3;1,4)-β-glucan content.
31754200	10	29	from	trait	1581:1585	arg1	grain					1601:1605	developing grain	1590:1605	developing grain	1590:1605	Our data emphasise the genotype-dependent accumulation of (1,3;1,4)-β-glucan during barley grain development and a role for the balance between hydrolysis and synthesis in determining (1,3;1,4)-β-glucan content, and suggests that other regulatory sequences or proteins are likely to be involved in this trait in developing grain.
31754200	4	30	theme	grain	480:484	arg1	synthase					505:512	a grain (1,3;1,4)-β-glucan synthase	478:512	a grain (1,3;1,4)-β-glucan synthase	478:512	HvCslF6 encodes a grain (1,3;1,4)-β-glucan synthase, whereas the function of HvCslF9 is unknown.
31754200	4	30	theme	grain	480:484	arg1	HvCslF6					462:468	HvCslF6	462:468	HvCslF6	462:468	HvCslF6 encodes a grain (1,3;1,4)-β-glucan synthase, whereas the function of HvCslF9 is unknown.
31754200	9	31	from	variation	1237:1245	arg1	content					1269:1275	(1,3;1,4)-β-glucan content	1250:1275	(1,3;1,4)-β-glucan content	1250:1275	Remarkably narrow sequence variation was found within the HvCslF6 promoter and coding sequence and does not explain variation in (1,3;1,4)-β-glucan content.
31754200	0	32	dep	content	32:38	arg1	effects					41:47	effects	41:47	Barley grain (1,3;1,4)-β-glucan content: effects of transcript and sequence variation in genes encoding the corresponding synthase and endohydrolase enzymes.	0:156	Barley grain (1,3;1,4)-β-glucan content: effects of transcript and sequence variation in genes encoding the corresponding synthase and endohydrolase enzymes.
31754200	9	33	dep	1,3	1251:1253	arg1	1,4					1255:1257	1,4	1255:1257	1,3;1,4	1251:1257	Remarkably narrow sequence variation was found within the HvCslF6 promoter and coding sequence and does not explain variation in (1,3;1,4)-β-glucan content.
31754200	3	34	theme	synthase-like	392:404	arg1	genes					406:410	synthase-like genes	392:410	synthase-like genes	392:410	Previous work showed Cellulose synthase-like genes synthesise (1,3;1,4)-β-glucan in several tissues.
31754200	6	35	theme	HvCslF9	862:868	arg1	transcript					870:879	HvCslF9 transcript	862:879	HvCslF9 transcript	862:879	HvCslF6 was differentially expressed during mid (8-15 DPA) and late (38 DPA) grain development stages while HvCslF9 transcript was only clearly detected at 8-10 DPA.
31754200	10	36	dep	1,3	1463:1465	arg1	1,4					1467:1469	1,4	1467:1469	1,4	1467:1469	Our data emphasise the genotype-dependent accumulation of (1,3;1,4)-β-glucan during barley grain development and a role for the balance between hydrolysis and synthesis in determining (1,3;1,4)-β-glucan content, and suggests that other regulatory sequences or proteins are likely to be involved in this trait in developing grain.
31754200	10	37	theme	-β-glucan	1471:1479	arg1	content					1481:1487	(1,3;1,4)-β-glucan content	1462:1487	(1,3;1,4)-β-glucan content	1462:1487	Our data emphasise the genotype-dependent accumulation of (1,3;1,4)-β-glucan during barley grain development and a role for the balance between hydrolysis and synthesis in determining (1,3;1,4)-β-glucan content, and suggests that other regulatory sequences or proteins are likely to be involved in this trait in developing grain.
31754200	9	38	theme	HvCslF6	1179:1185	arg1	promoter					1187:1194	the HvCslF6 promoter	1175:1194	the HvCslF6 promoter	1175:1194	Remarkably narrow sequence variation was found within the HvCslF6 promoter and coding sequence and does not explain variation in (1,3;1,4)-β-glucan content.
31754200	3	39	dep	Cellulose	382:390	arg1	synthesise					412:421	synthesise	412:421	synthesise (1,3;1,4)-β-glucan in several tissues	412:459	Previous work showed Cellulose synthase-like genes synthesise (1,3;1,4)-β-glucan in several tissues.
31754200	10	40	theme	genotype-dependent	1301:1318	arg1	accumulation					1320:1331	the genotype-dependent accumulation	1297:1331	the genotype-dependent accumulation of (1,3;1,4)-β-glucan	1297:1353	Our data emphasise the genotype-dependent accumulation of (1,3;1,4)-β-glucan during barley grain development and a role for the balance between hydrolysis and synthesis in determining (1,3;1,4)-β-glucan content, and suggests that other regulatory sequences or proteins are likely to be involved in this trait in developing grain.
31754200	8	41	from	Differences	972:982	arg1	abundance					998:1006	transcript abundance	987:1006	transcript abundance	987:1006	Differences in transcript abundance across the three genes could partially explain variation in grain (1,3;1,4)-β-glucan content in these genotypes.
31754200	3	42	theme	several	445:451	arg1	tissues					453:459	several tissues	445:459	several tissues	445:459	Previous work showed Cellulose synthase-like genes synthesise (1,3;1,4)-β-glucan in several tissues.
31754200	7	43	theme	expression	937:946	arg1	peak					922:925	A peak	920:925	A peak of HvGlbI expression	920:946	A peak of HvGlbI expression was detected at 15 DPA.
31754200	1	44	theme	cereal	222:227	arg1	end					229:231	cereal end uses	222:236	cereal end uses	222:236	The composition of plant cell walls is important in determining cereal end uses.
31754200	2	45	from	rich	306:309	arg1	-β-glucan					323:331	-β-glucan	323:331	-β-glucan	323:331	Unlike other widely consumed cereal grains barley is comparatively rich in (1,3;1,4)-β-glucan, a source of dietary fibre.
31754200	2	45	from	rich	306:309	arg1	source					336:341	a source	334:341	a source of dietary fibre	334:358	Unlike other widely consumed cereal grains barley is comparatively rich in (1,3;1,4)-β-glucan, a source of dietary fibre.
31754200	10	46	theme	barley	1362:1367	arg1	development					1375:1385	barley grain development	1362:1385	barley grain development	1362:1385	Our data emphasise the genotype-dependent accumulation of (1,3;1,4)-β-glucan during barley grain development and a role for the balance between hydrolysis and synthesis in determining (1,3;1,4)-β-glucan content, and suggests that other regulatory sequences or proteins are likely to be involved in this trait in developing grain.
31754200	2	47	theme	consumed	259:266	arg1	grains					275:280	other widely consumed cereal grains	246:280	other widely consumed cereal grains	246:280	Unlike other widely consumed cereal grains barley is comparatively rich in (1,3;1,4)-β-glucan, a source of dietary fibre.
31754200	4	48	theme	-β-glucan	495:503	arg1	synthase					505:512	a grain (1,3;1,4)-β-glucan synthase	478:512	a grain (1,3;1,4)-β-glucan synthase	478:512	HvCslF6 encodes a grain (1,3;1,4)-β-glucan synthase, whereas the function of HvCslF9 is unknown.
31754200	4	48	theme	-β-glucan	495:503	arg1	HvCslF6					462:468	HvCslF6	462:468	HvCslF6	462:468	HvCslF6 encodes a grain (1,3;1,4)-β-glucan synthase, whereas the function of HvCslF9 is unknown.
31754200	10	49	theme	-β-glucan	1345:1353	arg1	accumulation					1320:1331	the genotype-dependent accumulation	1297:1331	the genotype-dependent accumulation of (1,3;1,4)-β-glucan	1297:1353	Our data emphasise the genotype-dependent accumulation of (1,3;1,4)-β-glucan during barley grain development and a role for the balance between hydrolysis and synthesis in determining (1,3;1,4)-β-glucan content, and suggests that other regulatory sequences or proteins are likely to be involved in this trait in developing grain.
31754200	7	50	located	detected	952:959	arg1	DPA					967:969	15 DPA	964:969	15 DPA	964:969	A peak of HvGlbI expression was detected at 15 DPA.
31754200	7	50	located	detected	952:959	arg2	peak					922:925	A peak	920:925	A peak of HvGlbI expression	920:946	A peak of HvGlbI expression was detected at 15 DPA.
31754200	2	51	theme	other	246:250	arg1	grains					275:280	other widely consumed cereal grains	246:280	other widely consumed cereal grains	246:280	Unlike other widely consumed cereal grains barley is comparatively rich in (1,3;1,4)-β-glucan, a source of dietary fibre.
31754200	8	52	theme	grain	1068:1072	arg1	content					1093:1099	grain (1,3;1,4)-β-glucan content	1068:1099	grain (1,3;1,4)-β-glucan content in these genotypes	1068:1118	Differences in transcript abundance across the three genes could partially explain variation in grain (1,3;1,4)-β-glucan content in these genotypes.
31754200	10	53	dep	-β-glucan	1345:1353	arg1	1,3					1337:1339	1,3	1337:1339	1,3;1,4	1337:1343	Our data emphasise the genotype-dependent accumulation of (1,3;1,4)-β-glucan during barley grain development and a role for the balance between hydrolysis and synthesis in determining (1,3;1,4)-β-glucan content, and suggests that other regulatory sequences or proteins are likely to be involved in this trait in developing grain.
31754200	5	54	theme	-β-glucan	677:685	arg1	content					687:693	(1,3;1,4)-β-glucan content	668:693	(1,3;1,4)-β-glucan content	668:693	Here, the relationship between mRNA levels of HvCslF6, HvCslF9, HvGlbI (1,3;1,4)-β-glucan endohydrolase, and (1,3;1,4)-β-glucan content was studied in developing grains of four barley cultivars.
31754200	6	55	dep	mid	798:800	arg1	stages					849:854	grain development stages	831:854	grain development stages	831:854	HvCslF6 was differentially expressed during mid (8-15 DPA) and late (38 DPA) grain development stages while HvCslF9 transcript was only clearly detected at 8-10 DPA.
31754200	1	56	theme	cell	183:186	arg1	walls					188:192	plant cell walls	177:192	plant cell walls	177:192	The composition of plant cell walls is important in determining cereal end uses.
31754200	0	57	theme	synthase	122:129	arg1	enzymes					149:155	the corresponding synthase and endohydrolase enzymes	104:155	the corresponding synthase and endohydrolase enzymes	104:155	Barley grain (1,3;1,4)-β-glucan content: effects of transcript and sequence variation in genes encoding the corresponding synthase and endohydrolase enzymes.
31754200	9	58	located	found	1162:1166	arg1	sequence					1207:1214	coding sequence	1200:1214	coding sequence	1200:1214	Remarkably narrow sequence variation was found within the HvCslF6 promoter and coding sequence and does not explain variation in (1,3;1,4)-β-glucan content.
31754200	9	58	located	found	1162:1166	arg2	variation					1148:1156	Remarkably narrow sequence variation	1121:1156	Remarkably narrow sequence variation	1121:1156	Remarkably narrow sequence variation was found within the HvCslF6 promoter and coding sequence and does not explain variation in (1,3;1,4)-β-glucan content.
31754200	9	58	located	found	1162:1166	arg1	promoter					1187:1194	the HvCslF6 promoter	1175:1194	the HvCslF6 promoter	1175:1194	Remarkably narrow sequence variation was found within the HvCslF6 promoter and coding sequence and does not explain variation in (1,3;1,4)-β-glucan content.
31754200	8	59	theme	-β-glucan	1083:1091	arg1	content					1093:1099	grain (1,3;1,4)-β-glucan content	1068:1099	grain (1,3;1,4)-β-glucan content in these genotypes	1068:1118	Differences in transcript abundance across the three genes could partially explain variation in grain (1,3;1,4)-β-glucan content in these genotypes.
31754200	0	60	theme	endohydrolase	135:147	arg1	enzymes					149:155	the corresponding synthase and endohydrolase enzymes	104:155	the corresponding synthase and endohydrolase enzymes	104:155	Barley grain (1,3;1,4)-β-glucan content: effects of transcript and sequence variation in genes encoding the corresponding synthase and endohydrolase enzymes.
31754200	10	61	theme	developing	1590:1599	arg1	grain					1601:1605	developing grain	1590:1605	developing grain	1590:1605	Our data emphasise the genotype-dependent accumulation of (1,3;1,4)-β-glucan during barley grain development and a role for the balance between hydrolysis and synthesis in determining (1,3;1,4)-β-glucan content, and suggests that other regulatory sequences or proteins are likely to be involved in this trait in developing grain.
31754200	8	62	from	variation	1055:1063	arg1	content					1093:1099	grain (1,3;1,4)-β-glucan content	1068:1099	grain (1,3;1,4)-β-glucan content in these genotypes	1068:1118	Differences in transcript abundance across the three genes could partially explain variation in grain (1,3;1,4)-β-glucan content in these genotypes.
31754200	6	63	theme	development	837:847	arg1	stages					849:854	grain development stages	831:854	grain development stages	831:854	HvCslF6 was differentially expressed during mid (8-15 DPA) and late (38 DPA) grain development stages while HvCslF9 transcript was only clearly detected at 8-10 DPA.
31754200	5	64	theme	HvGlbI	623:628	arg1	endohydrolase					649:661	HvCslF9, HvGlbI (1,3;1,4)-β-glucan endohydrolase	614:661	HvCslF9, HvGlbI (1,3;1,4)-β-glucan endohydrolase	614:661	Here, the relationship between mRNA levels of HvCslF6, HvCslF9, HvGlbI (1,3;1,4)-β-glucan endohydrolase, and (1,3;1,4)-β-glucan content was studied in developing grains of four barley cultivars.
31754200	4	65	dep	encodes	470:476	arg1	whereas					515:521	whereas	515:521	whereas	515:521	HvCslF6 encodes a grain (1,3;1,4)-β-glucan synthase, whereas the function of HvCslF9 is unknown.
31754200	5	66	theme	cultivars	743:751	arg1	grains					721:726	developing grains	710:726	developing grains of four barley cultivars	710:751	Here, the relationship between mRNA levels of HvCslF6, HvCslF9, HvGlbI (1,3;1,4)-β-glucan endohydrolase, and (1,3;1,4)-β-glucan content was studied in developing grains of four barley cultivars.
31754200	2	67	dep	-β-glucan	323:331	arg1	1,3					315:317	1,3	315:317	1,3;1,4	315:321	Unlike other widely consumed cereal grains barley is comparatively rich in (1,3;1,4)-β-glucan, a source of dietary fibre.
31754200	3	68	dep	1,3	424:426	arg1	1,4					428:430	1,4	428:430	1,4	428:430	Previous work showed Cellulose synthase-like genes synthesise (1,3;1,4)-β-glucan in several tissues.
31754200	10	69	dep	content	1481:1487	arg1	1,3					1463:1465	1,3	1463:1465	1,3;1,4	1463:1469	Our data emphasise the genotype-dependent accumulation of (1,3;1,4)-β-glucan during barley grain development and a role for the balance between hydrolysis and synthesis in determining (1,3;1,4)-β-glucan content, and suggests that other regulatory sequences or proteins are likely to be involved in this trait in developing grain.
31754200	2	70	dep	1,3	315:317	arg1	1,4					319:321	1,4	319:321	1,4	319:321	Unlike other widely consumed cereal grains barley is comparatively rich in (1,3;1,4)-β-glucan, a source of dietary fibre.
31754200	0	71	theme	variation	76:84	arg1	effects					41:47	effects	41:47	Barley grain (1,3;1,4)-β-glucan content: effects of transcript and sequence variation in genes encoding the corresponding synthase and endohydrolase enzymes.	0:156	Barley grain (1,3;1,4)-β-glucan content: effects of transcript and sequence variation in genes encoding the corresponding synthase and endohydrolase enzymes.
31754200	4	72	dep	1,3	487:489	arg1	1,4					491:493	1,4	491:493	1,4	491:493	HvCslF6 encodes a grain (1,3;1,4)-β-glucan synthase, whereas the function of HvCslF9 is unknown.
31754200	1	73	theme	plant	177:181	arg1	walls					188:192	plant cell walls	177:192	plant cell walls	177:192	The composition of plant cell walls is important in determining cereal end uses.
31754200	0	74	theme	grain	7:11	arg1	content					32:38	Barley grain (1,3;1,4)-β-glucan content	0:38	Barley grain (1,3;1,4)-β-glucan content: effects of transcript and sequence variation in genes encoding the corresponding synthase and endohydrolase enzymes.	0:156	Barley grain (1,3;1,4)-β-glucan content: effects of transcript and sequence variation in genes encoding the corresponding synthase and endohydrolase enzymes.
31754200	4	75	dep	grain	480:484	arg1	1,3					487:489	1,3	487:489	1,3;1,4	487:493	HvCslF6 encodes a grain (1,3;1,4)-β-glucan synthase, whereas the function of HvCslF9 is unknown.
31754200	3	76	dep	-β-glucan	432:440	arg1	1,3					424:426	1,3	424:426	1,3	424:426	Previous work showed Cellulose synthase-like genes synthesise (1,3;1,4)-β-glucan in several tissues.
31754200	0	77	dep	grain	7:11	arg1	1,3					14:16	1,3	14:16	1,3;1,4	14:20	Barley grain (1,3;1,4)-β-glucan content: effects of transcript and sequence variation in genes encoding the corresponding synthase and endohydrolase enzymes.
31754200	2	78	theme	fibre	354:358	arg1	-β-glucan					323:331	-β-glucan	323:331	-β-glucan	323:331	Unlike other widely consumed cereal grains barley is comparatively rich in (1,3;1,4)-β-glucan, a source of dietary fibre.
31754200	2	78	theme	fibre	354:358	arg1	source					336:341	a source	334:341	a source of dietary fibre	334:358	Unlike other widely consumed cereal grains barley is comparatively rich in (1,3;1,4)-β-glucan, a source of dietary fibre.
31754200	5	79	theme	endohydrolase	649:661	arg1	levels					595:600	mRNA levels	590:600	mRNA levels of HvCslF6, HvCslF9, HvGlbI (1,3;1,4)-β-glucan endohydrolase, and (1,3;1,4)-β-glucan content	590:693	Here, the relationship between mRNA levels of HvCslF6, HvCslF9, HvGlbI (1,3;1,4)-β-glucan endohydrolase, and (1,3;1,4)-β-glucan content was studied in developing grains of four barley cultivars.
31754200	9	80	theme	-β-glucan	1259:1267	arg1	content					1269:1275	(1,3;1,4)-β-glucan content	1250:1275	(1,3;1,4)-β-glucan content	1250:1275	Remarkably narrow sequence variation was found within the HvCslF6 promoter and coding sequence and does not explain variation in (1,3;1,4)-β-glucan content.
31754200	0	81	theme	-β-glucan	22:30	arg1	content					32:38	Barley grain (1,3;1,4)-β-glucan content	0:38	Barley grain (1,3;1,4)-β-glucan content: effects of transcript and sequence variation in genes encoding the corresponding synthase and endohydrolase enzymes.	0:156	Barley grain (1,3;1,4)-β-glucan content: effects of transcript and sequence variation in genes encoding the corresponding synthase and endohydrolase enzymes.
31754200	2	82	from	-β-glucan	323:331	arg1	rich					306:309	rich	306:309	rich	306:309	Unlike other widely consumed cereal grains barley is comparatively rich in (1,3;1,4)-β-glucan, a source of dietary fibre.
31754200	0	83	dep	1,3	14:16	arg1	1,4					18:20	1,4	18:20	1,3;1,4	14:20	Barley grain (1,3;1,4)-β-glucan content: effects of transcript and sequence variation in genes encoding the corresponding synthase and endohydrolase enzymes.
31754200	9	84	dep	content	1269:1275	arg1	1,3					1251:1253	1,3	1251:1253	1,3;1,4	1251:1257	Remarkably narrow sequence variation was found within the HvCslF6 promoter and coding sequence and does not explain variation in (1,3;1,4)-β-glucan content.
31754200	9	85	theme	coding	1200:1205	arg1	sequence					1207:1214	coding sequence	1200:1214	coding sequence	1200:1214	Remarkably narrow sequence variation was found within the HvCslF6 promoter and coding sequence and does not explain variation in (1,3;1,4)-β-glucan content.
31754200	8	86	dep	content	1093:1099	arg1	1,3					1075:1077	1,3	1075:1077	1,3;1,4	1075:1081	Differences in transcript abundance across the three genes could partially explain variation in grain (1,3;1,4)-β-glucan content in these genotypes.
31754200	5	87	dep	content	687:693	arg1	1,3					669:671	1,3	669:671	1,3;1,4	669:675	Here, the relationship between mRNA levels of HvCslF6, HvCslF9, HvGlbI (1,3;1,4)-β-glucan endohydrolase, and (1,3;1,4)-β-glucan content was studied in developing grains of four barley cultivars.
30946740	9	0	theme	evolution	1643:1651	arg1	models					1629:1634	classic models	1621:1634	classic models of the evolution of novel protein functions	1621:1678	In contrast to classic models of the evolution of novel protein functions, the recombination breakpoints occurred within a single functional domain.
30946740	5	1	dep	confer	994:999	arg1	passaging					1059:1067	passaging	1059:1067	passaging	1059:1067	In one strain of S. eubayanus, we found a gene closely related to a known maltotriose transporter and were able to confer maltotriose consumption by overexpressing this gene or by passaging the strain on maltose.
30946740	5	1	dep	confer	994:999	arg1	overexpressing					1028:1041	overexpressing	1028:1041	overexpressing this gene	1028:1051	In one strain of S. eubayanus, we found a gene closely related to a known maltotriose transporter and were able to confer maltotriose consumption by overexpressing this gene or by passaging the strain on maltose.
30946740	7	2	theme	able	1370:1373	arg1	strain					1347:1352	a single strain	1338:1352	a single strain of S. eubayanus able to grow on maltotriose	1338:1396	To determine how this rare trait could evolve in naive genetic backgrounds, we performed an adaptive evolution experiment for maltotriose consumption, which yielded a single strain of S. eubayanus able to grow on maltotriose.
30946740	10	3	theme	evolved	1886:1892	arg1	substitutions					1894:1906	independently evolved substitutions	1872:1906	independently evolved substitutions	1872:1906	Thus, the ability of the new protein to carry maltotriose was likely acquired through epistatic interactions between independently evolved substitutions.
30946740	3	4	theme	limiting	526:533	arg1	uptake					465:470	the uptake	461:470	the uptake of the sugar maltotriose into the cell	461:509	In Saccharomyces, the uptake of the sugar maltotriose into the cell is the primary limiting factor in its utilization, but maltotriose transporters are relatively rare, except in brewing strains.
30946740	3	4	theme	limiting	526:533	arg1	factor					535:540	the primary limiting factor	514:540	the primary limiting factor in its utilization	514:559	In Saccharomyces, the uptake of the sugar maltotriose into the cell is the primary limiting factor in its utilization, but maltotriose transporters are relatively rare, except in brewing strains.
30946740	6	5	theme	wild	1106:1109	arg1	strains					1111:1117	most wild strains	1101:1117	most wild strains of S. eubayanus	1101:1133	Even so, most wild strains of S. eubayanus lack native maltotriose transporters.
30946740	3	6	from	strains	630:636	arg1	rare					606:609	rare	606:609	rare	606:609	In Saccharomyces, the uptake of the sugar maltotriose into the cell is the primary limiting factor in its utilization, but maltotriose transporters are relatively rare, except in brewing strains.
30946740	9	7	theme	functional	1736:1745	arg1	domain					1747:1752	a single functional domain	1727:1752	a single functional domain	1727:1752	In contrast to classic models of the evolution of novel protein functions, the recombination breakpoints occurred within a single functional domain.
30946740	4	8	theme	wild	648:651	arg1	strains					653:659	No known wild strains	639:659	No known wild strains of Saccharomyces eubayanus, the cold-tolerant parent of hybrid lager-brewing yeasts (Saccharomyces cerevisiae x S. eubayanus),	639:786	No known wild strains of Saccharomyces eubayanus, the cold-tolerant parent of hybrid lager-brewing yeasts (Saccharomyces cerevisiae x S. eubayanus), are able to consume maltotriose, which limits their ability to fully ferment malt extract.
30946740	1	9	theme	new	176:178	arg1	traits					180:185	new traits	176:185	new traits	176:185	At the molecular level, the evolution of new traits can be broadly divided between changes in gene expression and changes in protein-coding sequence.
30946740	9	10	theme	classic	1621:1627	arg1	models					1629:1634	classic models	1621:1634	classic models of the evolution of novel protein functions	1621:1678	In contrast to classic models of the evolution of novel protein functions, the recombination breakpoints occurred within a single functional domain.
30946740	1	11	from	changes	249:255	arg1	sequence					275:282	protein-coding sequence	260:282	protein-coding sequence	260:282	At the molecular level, the evolution of new traits can be broadly divided between changes in gene expression and changes in protein-coding sequence.
30946740	1	11	from	changes	249:255	arg1	expression					234:243	gene expression	229:243	gene expression	229:243	At the molecular level, the evolution of new traits can be broadly divided between changes in gene expression and changes in protein-coding sequence.
30946740	4	12	theme	malt	865:868	arg1	extract					870:876	malt extract	865:876	malt extract	865:876	No known wild strains of Saccharomyces eubayanus, the cold-tolerant parent of hybrid lager-brewing yeasts (Saccharomyces cerevisiae x S. eubayanus), are able to consume maltotriose, which limits their ability to fully ferment malt extract.
30946740	9	13	theme	novel	1656:1660	arg1	functions					1670:1678	novel protein functions	1656:1678	novel protein functions	1656:1678	In contrast to classic models of the evolution of novel protein functions, the recombination breakpoints occurred within a single functional domain.
30946740	11	14	theme	deleterious	2027:2037	arg1	steps					2052:2056	potentially deleterious intermediate steps	2015:2056	potentially deleterious intermediate steps	2015:2056	By acquiring multiple mutations at once, the transporter rapidly gained a novel function, while bypassing potentially deleterious intermediate steps.
30946740	6	15	theme	native	1140:1145	arg1	transporters					1159:1170	native maltotriose transporters	1140:1170	native maltotriose transporters	1140:1170	Even so, most wild strains of S. eubayanus lack native maltotriose transporters.
30946740	12	16	theme	adaptive	2204:2211	arg1	functions					2213:2221	adaptive functions	2204:2221	adaptive functions	2204:2221	This study provides an illuminating example of how recombination between paralogs can establish novel interactions among substitutions to create adaptive functions.
30946740	9	17	theme	functions	1670:1678	arg1	evolution					1643:1651	the evolution	1639:1651	the evolution of novel protein functions	1639:1678	In contrast to classic models of the evolution of novel protein functions, the recombination breakpoints occurred within a single functional domain.
30946740	0	18	from	proteins	93:100	arg1	Evolution					0:8	Evolution	0:8	Evolution of a novel chimeric maltotriose transporter in Saccharomyces eubayanus from parent proteins	0:100	Evolution of a novel chimeric maltotriose transporter in Saccharomyces eubayanus from parent proteins unable to perform this function.
30946740	0	18	from	proteins	93:100	arg1	transporter					42:52	a novel chimeric maltotriose transporter	13:52	a novel chimeric maltotriose transporter in Saccharomyces eubayanus from parent proteins	13:100	Evolution of a novel chimeric maltotriose transporter in Saccharomyces eubayanus from parent proteins unable to perform this function.
30946740	7	19	theme	evolution	1274:1282	arg1	experiment					1284:1293	an adaptive evolution experiment	1262:1293	an adaptive evolution experiment	1262:1293	To determine how this rare trait could evolve in naive genetic backgrounds, we performed an adaptive evolution experiment for maltotriose consumption, which yielded a single strain of S. eubayanus able to grow on maltotriose.
30946740	1	20	theme	molecular	142:150	arg1	level					152:156	the molecular level	138:156	the molecular level	138:156	At the molecular level, the evolution of new traits can be broadly divided between changes in gene expression and changes in protein-coding sequence.
30946740	11	21	theme	intermediate	2039:2050	arg1	steps					2052:2056	potentially deleterious intermediate steps	2015:2056	potentially deleterious intermediate steps	2015:2056	By acquiring multiple mutations at once, the transporter rapidly gained a novel function, while bypassing potentially deleterious intermediate steps.
30946740	4	22	theme	eubayanus	678:686	arg1	strains					653:659	No known wild strains	639:659	No known wild strains of Saccharomyces eubayanus, the cold-tolerant parent of hybrid lager-brewing yeasts (Saccharomyces cerevisiae x S. eubayanus),	639:786	No known wild strains of Saccharomyces eubayanus, the cold-tolerant parent of hybrid lager-brewing yeasts (Saccharomyces cerevisiae x S. eubayanus), are able to consume maltotriose, which limits their ability to fully ferment malt extract.
30946740	9	23	dep	models	1629:1634	arg1	contrast					1609:1616	contrast	1609:1616	contrast	1609:1616	In contrast to classic models of the evolution of novel protein functions, the recombination breakpoints occurred within a single functional domain.
30946740	3	24	theme	brewing	622:628	arg1	strains					630:636	brewing strains	622:636	brewing strains	622:636	In Saccharomyces, the uptake of the sugar maltotriose into the cell is the primary limiting factor in its utilization, but maltotriose transporters are relatively rare, except in brewing strains.
30946740	0	25	from	Evolution	0:8	arg1	eubayanus					71:79	Saccharomyces eubayanus	57:79	Saccharomyces eubayanus	57:79	Evolution of a novel chimeric maltotriose transporter in Saccharomyces eubayanus from parent proteins unable to perform this function.
30946740	0	25	from	Evolution	0:8	arg1	proteins					93:100	parent proteins	86:100	parent proteins	86:100	Evolution of a novel chimeric maltotriose transporter in Saccharomyces eubayanus from parent proteins unable to perform this function.
30946740	0	26	from	transporter	42:52	arg1	eubayanus					71:79	Saccharomyces eubayanus	57:79	Saccharomyces eubayanus	57:79	Evolution of a novel chimeric maltotriose transporter in Saccharomyces eubayanus from parent proteins unable to perform this function.
30946740	2	27	theme	point	383:387	arg1	mutations					389:397	sequential point mutations	372:397	sequential point mutations	372:397	For proteins, the evolution of novel functions is generally thought to proceed through sequential point mutations or recombination of whole functional units.
30946740	7	28	theme	adaptive	1265:1272	arg1	experiment					1284:1293	an adaptive evolution experiment	1262:1293	an adaptive evolution experiment	1262:1293	To determine how this rare trait could evolve in naive genetic backgrounds, we performed an adaptive evolution experiment for maltotriose consumption, which yielded a single strain of S. eubayanus able to grow on maltotriose.
30946740	9	29	theme	single	1729:1734	arg1	domain					1747:1752	a single functional domain	1727:1752	a single functional domain	1727:1752	In contrast to classic models of the evolution of novel protein functions, the recombination breakpoints occurred within a single functional domain.
30946740	8	30	theme	novel	1450:1454	arg1	transporter					1465:1475	a novel chimeric transporter	1448:1475	a novel chimeric transporter that was formed by an ectopic recombination event between two genes encoding transporters that are unable to import maltotriose	1448:1603	We mapped the causative locus to a gene encoding a novel chimeric transporter that was formed by an ectopic recombination event between two genes encoding transporters that are unable to import maltotriose.
30946740	4	31	theme	hybrid	717:722	arg1	yeasts					738:743	hybrid lager-brewing yeasts	717:743	hybrid lager-brewing yeasts (Saccharomyces cerevisiae x S. eubayanus)	717:785	No known wild strains of Saccharomyces eubayanus, the cold-tolerant parent of hybrid lager-brewing yeasts (Saccharomyces cerevisiae x S. eubayanus), are able to consume maltotriose, which limits their ability to fully ferment malt extract.
30946740	5	32	from	passaging	1059:1067	arg1	maltose					1083:1089	maltose	1083:1089	maltose	1083:1089	In one strain of S. eubayanus, we found a gene closely related to a known maltotriose transporter and were able to confer maltotriose consumption by overexpressing this gene or by passaging the strain on maltose.
30946740	5	33	theme	eubayanus	899:907	arg1	strain					886:891	one strain	882:891	one strain of S. eubayanus	882:907	In one strain of S. eubayanus, we found a gene closely related to a known maltotriose transporter and were able to confer maltotriose consumption by overexpressing this gene or by passaging the strain on maltose.
30946740	2	34	theme	functional	425:434	arg1	units					436:440	whole functional units	419:440	whole functional units	419:440	For proteins, the evolution of novel functions is generally thought to proceed through sequential point mutations or recombination of whole functional units.
30946740	6	35	theme	eubayanus	1125:1133	arg1	strains					1111:1117	most wild strains	1101:1117	most wild strains of S. eubayanus	1101:1133	Even so, most wild strains of S. eubayanus lack native maltotriose transporters.
30946740	4	36	theme	yeasts	738:743	arg1	eubayanus					678:686	Saccharomyces eubayanus	664:686	Saccharomyces eubayanus	664:686	No known wild strains of Saccharomyces eubayanus, the cold-tolerant parent of hybrid lager-brewing yeasts (Saccharomyces cerevisiae x S. eubayanus), are able to consume maltotriose, which limits their ability to fully ferment malt extract.
30946740	4	36	theme	yeasts	738:743	arg1	parent					707:712	the cold-tolerant parent	689:712	the cold-tolerant parent of hybrid lager-brewing yeasts (Saccharomyces cerevisiae x S. eubayanus)	689:785	No known wild strains of Saccharomyces eubayanus, the cold-tolerant parent of hybrid lager-brewing yeasts (Saccharomyces cerevisiae x S. eubayanus), are able to consume maltotriose, which limits their ability to fully ferment malt extract.
30946740	10	37	theme	new	1780:1782	arg1	protein					1784:1790	the new protein	1776:1790	the new protein	1776:1790	Thus, the ability of the new protein to carry maltotriose was likely acquired through epistatic interactions between independently evolved substitutions.
30946740	12	38	theme	novel	2155:2159	arg1	interactions					2161:2172	novel interactions	2155:2172	novel interactions among substitutions	2155:2192	This study provides an illuminating example of how recombination between paralogs can establish novel interactions among substitutions to create adaptive functions.
30946740	0	39	theme	chimeric	21:28	arg1	transporter					42:52	a novel chimeric maltotriose transporter	13:52	a novel chimeric maltotriose transporter in Saccharomyces eubayanus from parent proteins	13:100	Evolution of a novel chimeric maltotriose transporter in Saccharomyces eubayanus from parent proteins unable to perform this function.
30946740	7	40	theme	genetic	1228:1234	arg1	backgrounds					1236:1246	naive genetic backgrounds	1222:1246	naive genetic backgrounds	1222:1246	To determine how this rare trait could evolve in naive genetic backgrounds, we performed an adaptive evolution experiment for maltotriose consumption, which yielded a single strain of S. eubayanus able to grow on maltotriose.
30946740	2	41	theme	functions	322:330	arg1	evolution					303:311	the evolution	299:311	the evolution of novel functions	299:330	For proteins, the evolution of novel functions is generally thought to proceed through sequential point mutations or recombination of whole functional units.
30946740	0	42	theme	transporter	42:52	arg1	Evolution					0:8	Evolution	0:8	Evolution of a novel chimeric maltotriose transporter in Saccharomyces eubayanus from parent proteins	0:100	Evolution of a novel chimeric maltotriose transporter in Saccharomyces eubayanus from parent proteins unable to perform this function.
30946740	8	43	theme	ectopic	1499:1505	arg1	event					1521:1525	an ectopic recombination event	1496:1525	an ectopic recombination event between two genes encoding transporters that are unable to import maltotriose	1496:1603	We mapped the causative locus to a gene encoding a novel chimeric transporter that was formed by an ectopic recombination event between two genes encoding transporters that are unable to import maltotriose.
30946740	6	44	theme	most	1101:1104	arg1	strains					1111:1117	most wild strains	1101:1117	most wild strains of S. eubayanus	1101:1133	Even so, most wild strains of S. eubayanus lack native maltotriose transporters.
30946740	8	45	theme	import	1586:1591	arg1	maltotriose					1593:1603	import maltotriose	1586:1603	import maltotriose	1586:1603	We mapped the causative locus to a gene encoding a novel chimeric transporter that was formed by an ectopic recombination event between two genes encoding transporters that are unable to import maltotriose.
30946740	5	46	theme	known	947:951	arg1	transporter					965:975	a known maltotriose transporter	945:975	a known maltotriose transporter	945:975	In one strain of S. eubayanus, we found a gene closely related to a known maltotriose transporter and were able to confer maltotriose consumption by overexpressing this gene or by passaging the strain on maltose.
30946740	7	47	theme	rare	1195:1198	arg1	trait					1200:1204	this rare trait	1190:1204	this rare trait	1190:1204	To determine how this rare trait could evolve in naive genetic backgrounds, we performed an adaptive evolution experiment for maltotriose consumption, which yielded a single strain of S. eubayanus able to grow on maltotriose.
30946740	3	48	theme	maltotriose	485:495	arg1	uptake					465:470	the uptake	461:470	the uptake of the sugar maltotriose into the cell	461:509	In Saccharomyces, the uptake of the sugar maltotriose into the cell is the primary limiting factor in its utilization, but maltotriose transporters are relatively rare, except in brewing strains.
30946740	3	48	theme	maltotriose	485:495	arg1	factor					535:540	the primary limiting factor	514:540	the primary limiting factor in its utilization	514:559	In Saccharomyces, the uptake of the sugar maltotriose into the cell is the primary limiting factor in its utilization, but maltotriose transporters are relatively rare, except in brewing strains.
30946740	1	49	theme	gene	229:232	arg1	expression					234:243	gene expression	229:243	gene expression	229:243	At the molecular level, the evolution of new traits can be broadly divided between changes in gene expression and changes in protein-coding sequence.
30946740	3	50	theme	primary	518:524	arg1	uptake					465:470	the uptake	461:470	the uptake of the sugar maltotriose into the cell	461:509	In Saccharomyces, the uptake of the sugar maltotriose into the cell is the primary limiting factor in its utilization, but maltotriose transporters are relatively rare, except in brewing strains.
30946740	3	50	theme	primary	518:524	arg1	factor					535:540	the primary limiting factor	514:540	the primary limiting factor in its utilization	514:559	In Saccharomyces, the uptake of the sugar maltotriose into the cell is the primary limiting factor in its utilization, but maltotriose transporters are relatively rare, except in brewing strains.
30946740	7	51	theme	eubayanus	1360:1368	arg1	strain					1347:1352	a single strain	1338:1352	a single strain of S. eubayanus able to grow on maltotriose	1338:1396	To determine how this rare trait could evolve in naive genetic backgrounds, we performed an adaptive evolution experiment for maltotriose consumption, which yielded a single strain of S. eubayanus able to grow on maltotriose.
30946740	12	52	theme	illuminating	2082:2093	arg1	example					2095:2101	an illuminating example	2079:2101	an illuminating example of how recombination between paralogs can establish novel interactions among substitutions to create adaptive functions	2079:2221	This study provides an illuminating example of how recombination between paralogs can establish novel interactions among substitutions to create adaptive functions.
30946740	11	53	theme	novel	1983:1987	arg1	function					1989:1996	a novel function	1981:1996	a novel function	1981:1996	By acquiring multiple mutations at once, the transporter rapidly gained a novel function, while bypassing potentially deleterious intermediate steps.
30946740	4	54	theme	known	642:646	arg1	strains					653:659	No known wild strains	639:659	No known wild strains of Saccharomyces eubayanus, the cold-tolerant parent of hybrid lager-brewing yeasts (Saccharomyces cerevisiae x S. eubayanus),	639:786	No known wild strains of Saccharomyces eubayanus, the cold-tolerant parent of hybrid lager-brewing yeasts (Saccharomyces cerevisiae x S. eubayanus), are able to consume maltotriose, which limits their ability to fully ferment malt extract.
30946740	1	55	theme	traits	180:185	arg1	evolution					163:171	the evolution	159:171	the evolution of new traits	159:185	At the molecular level, the evolution of new traits can be broadly divided between changes in gene expression and changes in protein-coding sequence.
30946740	7	56	theme	single	1340:1345	arg1	strain					1347:1352	a single strain	1338:1352	a single strain of S. eubayanus able to grow on maltotriose	1338:1396	To determine how this rare trait could evolve in naive genetic backgrounds, we performed an adaptive evolution experiment for maltotriose consumption, which yielded a single strain of S. eubayanus able to grow on maltotriose.
30946740	2	57	theme	novel	316:320	arg1	functions					322:330	novel functions	316:330	novel functions	316:330	For proteins, the evolution of novel functions is generally thought to proceed through sequential point mutations or recombination of whole functional units.
30946740	3	58	theme	maltotriose	566:576	arg1	transporters					578:589	maltotriose transporters	566:589	maltotriose transporters	566:589	In Saccharomyces, the uptake of the sugar maltotriose into the cell is the primary limiting factor in its utilization, but maltotriose transporters are relatively rare, except in brewing strains.
30946740	0	59	from	eubayanus	71:79	arg1	Evolution					0:8	Evolution	0:8	Evolution of a novel chimeric maltotriose transporter in Saccharomyces eubayanus from parent proteins	0:100	Evolution of a novel chimeric maltotriose transporter in Saccharomyces eubayanus from parent proteins unable to perform this function.
30946740	1	60	from	changes	218:224	arg1	sequence					275:282	protein-coding sequence	260:282	protein-coding sequence	260:282	At the molecular level, the evolution of new traits can be broadly divided between changes in gene expression and changes in protein-coding sequence.
30946740	1	60	from	changes	218:224	arg1	expression					234:243	gene expression	229:243	gene expression	229:243	At the molecular level, the evolution of new traits can be broadly divided between changes in gene expression and changes in protein-coding sequence.
30946740	4	61	dep	yeasts	738:743	arg1	cerevisiae					760:769	Saccharomyces cerevisiae	746:769	Saccharomyces cerevisiae	746:769	No known wild strains of Saccharomyces eubayanus, the cold-tolerant parent of hybrid lager-brewing yeasts (Saccharomyces cerevisiae x S. eubayanus), are able to consume maltotriose, which limits their ability to fully ferment malt extract.
30946740	4	61	dep	yeasts	738:743	arg1	eubayanus					776:784	S. eubayanus	773:784	S. eubayanus	773:784	No known wild strains of Saccharomyces eubayanus, the cold-tolerant parent of hybrid lager-brewing yeasts (Saccharomyces cerevisiae x S. eubayanus), are able to consume maltotriose, which limits their ability to fully ferment malt extract.
30946740	6	62	theme	maltotriose	1147:1157	arg1	transporters					1159:1170	native maltotriose transporters	1140:1170	native maltotriose transporters	1140:1170	Even so, most wild strains of S. eubayanus lack native maltotriose transporters.
30946740	3	63	from	factor	535:540	arg1	utilization					549:559	its utilization	545:559	its utilization	545:559	In Saccharomyces, the uptake of the sugar maltotriose into the cell is the primary limiting factor in its utilization, but maltotriose transporters are relatively rare, except in brewing strains.
30946740	9	64	theme	protein	1662:1668	arg1	functions					1670:1678	novel protein functions	1656:1678	novel protein functions	1656:1678	In contrast to classic models of the evolution of novel protein functions, the recombination breakpoints occurred within a single functional domain.
30946740	8	65	theme	causative	1413:1421	arg1	locus					1423:1427	the causative locus	1409:1427	the causative locus	1409:1427	We mapped the causative locus to a gene encoding a novel chimeric transporter that was formed by an ectopic recombination event between two genes encoding transporters that are unable to import maltotriose.
30946740	7	66	theme	maltotriose	1299:1309	arg1	consumption					1311:1321	maltotriose consumption	1299:1321	maltotriose consumption	1299:1321	To determine how this rare trait could evolve in naive genetic backgrounds, we performed an adaptive evolution experiment for maltotriose consumption, which yielded a single strain of S. eubayanus able to grow on maltotriose.
30946740	4	67	theme	cold-tolerant	693:705	arg1	eubayanus					678:686	Saccharomyces eubayanus	664:686	Saccharomyces eubayanus	664:686	No known wild strains of Saccharomyces eubayanus, the cold-tolerant parent of hybrid lager-brewing yeasts (Saccharomyces cerevisiae x S. eubayanus), are able to consume maltotriose, which limits their ability to fully ferment malt extract.
30946740	4	67	theme	cold-tolerant	693:705	arg1	parent					707:712	the cold-tolerant parent	689:712	the cold-tolerant parent of hybrid lager-brewing yeasts (Saccharomyces cerevisiae x S. eubayanus)	689:785	No known wild strains of Saccharomyces eubayanus, the cold-tolerant parent of hybrid lager-brewing yeasts (Saccharomyces cerevisiae x S. eubayanus), are able to consume maltotriose, which limits their ability to fully ferment malt extract.
30946740	5	68	theme	maltotriose	1001:1011	arg1	consumption					1013:1023	maltotriose consumption	1001:1023	maltotriose consumption	1001:1023	In one strain of S. eubayanus, we found a gene closely related to a known maltotriose transporter and were able to confer maltotriose consumption by overexpressing this gene or by passaging the strain on maltose.
30946740	11	69	theme	multiple	1922:1929	arg1	mutations					1931:1939	multiple mutations	1922:1939	multiple mutations	1922:1939	By acquiring multiple mutations at once, the transporter rapidly gained a novel function, while bypassing potentially deleterious intermediate steps.
30946740	0	70	theme	parent	86:91	arg1	proteins					93:100	parent proteins	86:100	parent proteins	86:100	Evolution of a novel chimeric maltotriose transporter in Saccharomyces eubayanus from parent proteins unable to perform this function.
30946740	9	71	theme	recombination	1685:1697	arg1	breakpoints					1699:1709	the recombination breakpoints	1681:1709	the recombination breakpoints	1681:1709	In contrast to classic models of the evolution of novel protein functions, the recombination breakpoints occurred within a single functional domain.
30946740	0	72	dep	perform	112:118	arg1	unable					102:107	unable	102:107	unable	102:107	Evolution of a novel chimeric maltotriose transporter in Saccharomyces eubayanus from parent proteins unable to perform this function.
30946740	0	72	dep	perform	112:118	arg1	Evolution					0:8	Evolution	0:8	Evolution of a novel chimeric maltotriose transporter in Saccharomyces eubayanus from parent proteins	0:100	Evolution of a novel chimeric maltotriose transporter in Saccharomyces eubayanus from parent proteins unable to perform this function.
30946740	8	73	theme	chimeric	1456:1463	arg1	transporter					1465:1475	a novel chimeric transporter	1448:1475	a novel chimeric transporter that was formed by an ectopic recombination event between two genes encoding transporters that are unable to import maltotriose	1448:1603	We mapped the causative locus to a gene encoding a novel chimeric transporter that was formed by an ectopic recombination event between two genes encoding transporters that are unable to import maltotriose.
30946740	3	74	from	rare	606:609	arg1	strains					630:636	brewing strains	622:636	brewing strains	622:636	In Saccharomyces, the uptake of the sugar maltotriose into the cell is the primary limiting factor in its utilization, but maltotriose transporters are relatively rare, except in brewing strains.
30946740	2	75	theme	sequential	372:381	arg1	mutations					389:397	sequential point mutations	372:397	sequential point mutations	372:397	For proteins, the evolution of novel functions is generally thought to proceed through sequential point mutations or recombination of whole functional units.
30946740	2	76	theme	units	436:440	arg1	recombination					402:414	recombination	402:414	recombination of whole functional units	402:440	For proteins, the evolution of novel functions is generally thought to proceed through sequential point mutations or recombination of whole functional units.
30946740	2	76	theme	units	436:440	arg1	mutations					389:397	sequential point mutations	372:397	sequential point mutations	372:397	For proteins, the evolution of novel functions is generally thought to proceed through sequential point mutations or recombination of whole functional units.
30946740	0	77	theme	novel	15:19	arg1	transporter					42:52	a novel chimeric maltotriose transporter	13:52	a novel chimeric maltotriose transporter in Saccharomyces eubayanus from parent proteins	13:100	Evolution of a novel chimeric maltotriose transporter in Saccharomyces eubayanus from parent proteins unable to perform this function.
30946740	1	78	theme	protein-coding	260:273	arg1	sequence					275:282	protein-coding sequence	260:282	protein-coding sequence	260:282	At the molecular level, the evolution of new traits can be broadly divided between changes in gene expression and changes in protein-coding sequence.
30946740	2	79	theme	whole	419:423	arg1	units					436:440	whole functional units	419:440	whole functional units	419:440	For proteins, the evolution of novel functions is generally thought to proceed through sequential point mutations or recombination of whole functional units.
30946740	10	80	theme	protein	1784:1790	arg1	ability					1765:1771	the ability	1761:1771	the ability of the new protein to carry maltotriose	1761:1811	Thus, the ability of the new protein to carry maltotriose was likely acquired through epistatic interactions between independently evolved substitutions.
30946740	4	81	theme	lager-brewing	724:736	arg1	yeasts					738:743	hybrid lager-brewing yeasts	717:743	hybrid lager-brewing yeasts (Saccharomyces cerevisiae x S. eubayanus)	717:785	No known wild strains of Saccharomyces eubayanus, the cold-tolerant parent of hybrid lager-brewing yeasts (Saccharomyces cerevisiae x S. eubayanus), are able to consume maltotriose, which limits their ability to fully ferment malt extract.
30946740	0	82	theme	maltotriose	30:40	arg1	transporter					42:52	a novel chimeric maltotriose transporter	13:52	a novel chimeric maltotriose transporter in Saccharomyces eubayanus from parent proteins	13:100	Evolution of a novel chimeric maltotriose transporter in Saccharomyces eubayanus from parent proteins unable to perform this function.
30946740	5	83	theme	maltotriose	953:963	arg1	transporter					965:975	a known maltotriose transporter	945:975	a known maltotriose transporter	945:975	In one strain of S. eubayanus, we found a gene closely related to a known maltotriose transporter and were able to confer maltotriose consumption by overexpressing this gene or by passaging the strain on maltose.
30946740	7	84	theme	naive	1222:1226	arg1	backgrounds					1236:1246	naive genetic backgrounds	1222:1246	naive genetic backgrounds	1222:1246	To determine how this rare trait could evolve in naive genetic backgrounds, we performed an adaptive evolution experiment for maltotriose consumption, which yielded a single strain of S. eubayanus able to grow on maltotriose.
30946740	8	85	theme	recombination	1507:1519	arg1	event					1521:1525	an ectopic recombination event	1496:1525	an ectopic recombination event between two genes encoding transporters that are unable to import maltotriose	1496:1603	We mapped the causative locus to a gene encoding a novel chimeric transporter that was formed by an ectopic recombination event between two genes encoding transporters that are unable to import maltotriose.
30946740	3	86	theme	sugar	479:483	arg1	maltotriose					485:495	the sugar maltotriose	475:495	the sugar maltotriose	475:495	In Saccharomyces, the uptake of the sugar maltotriose into the cell is the primary limiting factor in its utilization, but maltotriose transporters are relatively rare, except in brewing strains.
30946740	5	87	theme	related	934:940	arg1	gene					921:924	a gene	919:924	a gene closely related to a known maltotriose transporter	919:975	In one strain of S. eubayanus, we found a gene closely related to a known maltotriose transporter and were able to confer maltotriose consumption by overexpressing this gene or by passaging the strain on maltose.
30946740	10	88	theme	epistatic	1841:1849	arg1	interactions					1851:1862	epistatic interactions	1841:1862	epistatic interactions between independently evolved substitutions	1841:1906	Thus, the ability of the new protein to carry maltotriose was likely acquired through epistatic interactions between independently evolved substitutions.
30240938	4	0	theme	Cartilage	547:555	arg1	damage					557:562	DESIGN Cartilage damage	540:562	DESIGN Cartilage damage	540:562	DESIGN Cartilage damage was induced through cyclic compressive loading in four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT).
30240938	11	1	theme	milder	1518:1523	arg1	changes					1525:1531	milder changes	1518:1531	milder changes in adiposity/metabolic syndrome seen in TLR5KO mice	1518:1583	CONCLUSIONS Severe obesity increased load-induced cartilage damage, while milder changes in adiposity/metabolic syndrome seen in TLR5KO mice did not.
30240938	7	2	contain	had	1228:1230	arg2	damage					1242:1247	cartilage damage	1232:1247	cartilage damage comparable to WT mice	1232:1269	After 6 weeks of loading, HFD mice had increased load-induced cartilage damage, while TLR5KO mice had cartilage damage comparable to WT mice.
30240938	7	2	contain	had	1228:1230	arg1	mice					1223:1226	TLR5KO mice	1216:1226	TLR5KO mice	1216:1226	After 6 weeks of loading, HFD mice had increased load-induced cartilage damage, while TLR5KO mice had cartilage damage comparable to WT mice.
30240938	12	3	theme	cartilage	1655:1663	arg1	damage					1665:1670	cartilage damage	1655:1670	cartilage damage	1655:1670	Furthermore, the effects of systemic inflammation/obesity on cartilage damage depend on the duration of mechanical loading.
30240938	6	4	theme	cartilage	1067:1075	arg1	loading					1058:1064	loading	1058:1064	loading	1058:1064	RESULTS After 2 weeks of loading, cartilage damage (OARSI score) was not different among groups.
30240938	6	4	theme	cartilage	1067:1075	arg1	damage					1077:1082	cartilage damage	1067:1082	cartilage damage (OARSI score)	1067:1096	RESULTS After 2 weeks of loading, cartilage damage (OARSI score) was not different among groups.
30240938	6	4	theme	cartilage	1067:1075	arg1	score					1091:1095	OARSI score	1085:1095	OARSI score	1085:1095	RESULTS After 2 weeks of loading, cartilage damage (OARSI score) was not different among groups.
30240938	3	5	theme	microbiome	509:518	arg1	effects					457:463	the effects	453:463	the effects of altered metabolism, obesity, and the gut microbiome on load-induced OA	453:537	Here we examined the effects of altered metabolism, obesity, and the gut microbiome on load-induced OA.
30240938	6	6	theme	loading	1058:1064	arg1	weeks					1049:1053	2 weeks	1047:1053	2 weeks of loading, cartilage damage (OARSI score)	1047:1096	RESULTS After 2 weeks of loading, cartilage damage (OARSI score) was not different among groups.
30240938	4	7	theme	adult	629:633	arg1	mice					640:643	adult male mice	629:643	adult male mice	629:643	DESIGN Cartilage damage was induced through cyclic compressive loading in four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT).
30240938	10	8	theme	microbiome	1420:1429	arg1	composition					1431:1441	a distinct gut microbiome composition	1405:1441	a distinct gut microbiome composition	1405:1441	Each group had a distinct gut microbiome composition.
30240938	4	9	theme	mice	640:643	arg1	mice					942:945	untreated C57BL/6J mice	923:945	untreated C57BL/6J mice (WT)	923:950	DESIGN Cartilage damage was induced through cyclic compressive loading in four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT).
30240938	4	9	theme	mice	640:643	arg1	mice					686:689	Toll-like receptor-5 deficient (TLR5KO) mice	646:689	Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome	646:762	DESIGN Cartilage damage was induced through cyclic compressive loading in four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT).
30240938	4	9	theme	mice	640:643	arg1	groups					619:624	four groups	614:624	four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT)	614:950	DESIGN Cartilage damage was induced through cyclic compressive loading in four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT).
30240938	4	9	theme	mice	640:643	arg1	WT					948:949	WT	948:949	WT	948:949	DESIGN Cartilage damage was induced through cyclic compressive loading in four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT).
30240938	4	9	theme	mice	640:643	arg1	mice					772:775	TLR5KO mice	765:775	TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota)	765:858	DESIGN Cartilage damage was induced through cyclic compressive loading in four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT).
30240938	4	9	theme	mice	640:643	arg1	mice					870:873	C57BL/6J mice	861:873	C57BL/6J mice fed a high fat diet to cause obesity (HFD)	861:916	DESIGN Cartilage damage was induced through cyclic compressive loading in four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT).
30240938	4	9	theme	mice	640:643	arg1	mice					640:643	adult male mice	629:643	adult male mice	629:643	DESIGN Cartilage damage was induced through cyclic compressive loading in four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT).
30240938	12	10	theme	inflammation/obesity	1631:1650	arg1	effects					1611:1617	the effects	1607:1617	the effects of systemic inflammation/obesity on cartilage damage	1607:1670	Furthermore, the effects of systemic inflammation/obesity on cartilage damage depend on the duration of mechanical loading.
30240938	3	11	theme	load-induced	523:534	arg1	OA					536:537	load-induced OA	523:537	load-induced OA	523:537	Here we examined the effects of altered metabolism, obesity, and the gut microbiome on load-induced OA.
30240938	1	12	theme	Metabolic	109:117	arg1	syndrome					119:126	OBJECTIVE Metabolic syndrome	99:126	OBJECTIVE Metabolic syndrome	99:126	OBJECTIVE Metabolic syndrome is characterized by obesity, hyperglycemia, hypertension, insulin resistance, and dyslipidemia.
30240938	4	13	theme	fat	886:888	arg1	HFD					913:915	HFD	913:915	HFD	913:915	DESIGN Cartilage damage was induced through cyclic compressive loading in four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT).
30240938	4	13	theme	fat	886:888	arg1	diet					890:893	a high fat diet	879:893	a high fat diet to cause obesity (HFD)	879:916	DESIGN Cartilage damage was induced through cyclic compressive loading in four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT).
30240938	8	14	theme	cartilage	1304:1312	arg1	damage					1314:1319	less cartilage damage	1299:1319	less cartilage damage	1299:1319	TLR5KOΔMicrobiota mice had less cartilage damage than other groups.
30240938	12	15	from	effects	1611:1617	arg1	damage					1665:1670	cartilage damage	1655:1670	cartilage damage	1655:1670	Furthermore, the effects of systemic inflammation/obesity on cartilage damage depend on the duration of mechanical loading.
30240938	4	16	dep	diet	890:893	arg1	cause					898:902	cause	898:902	to cause obesity	895:910	DESIGN Cartilage damage was induced through cyclic compressive loading in four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT).
30240938	7	17	contain	had	1165:1167	arg1	mice					1160:1163	HFD mice	1156:1163	HFD mice	1156:1163	After 6 weeks of loading, HFD mice had increased load-induced cartilage damage, while TLR5KO mice had cartilage damage comparable to WT mice.
30240938	7	17	contain	had	1165:1167	arg2	damage					1202:1207	increased load-induced cartilage damage	1169:1207	increased load-induced cartilage damage	1169:1207	After 6 weeks of loading, HFD mice had increased load-induced cartilage damage, while TLR5KO mice had cartilage damage comparable to WT mice.
30240938	2	18	theme	Metabolic	224:232	arg1	syndrome					234:241	Metabolic syndrome	224:241	Metabolic syndrome	224:241	Metabolic syndrome is associated with osteoarthritis (OA), but it is unclear if the association is attributable to increased mechanical loading on joints caused by obesity or other aspects of metabolic syndrome.
30240938	9	19	theme	elevated	1353:1360	arg1	markers					1381:1387	elevated serum inflammatory markers	1353:1387	elevated serum inflammatory markers	1353:1387	HFD mice had elevated serum inflammatory markers.
30240938	4	20	theme	mice	870:873	arg1	mice					942:945	untreated C57BL/6J mice	923:945	untreated C57BL/6J mice (WT)	923:950	DESIGN Cartilage damage was induced through cyclic compressive loading in four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT).
30240938	4	20	theme	mice	870:873	arg1	mice					686:689	Toll-like receptor-5 deficient (TLR5KO) mice	646:689	Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome	646:762	DESIGN Cartilage damage was induced through cyclic compressive loading in four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT).
30240938	4	20	theme	mice	870:873	arg1	groups					619:624	four groups	614:624	four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT)	614:950	DESIGN Cartilage damage was induced through cyclic compressive loading in four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT).
30240938	4	20	theme	mice	870:873	arg1	WT					948:949	WT	948:949	WT	948:949	DESIGN Cartilage damage was induced through cyclic compressive loading in four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT).
30240938	4	20	theme	mice	870:873	arg1	mice					772:775	TLR5KO mice	765:775	TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota)	765:858	DESIGN Cartilage damage was induced through cyclic compressive loading in four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT).
30240938	4	20	theme	mice	870:873	arg1	mice					870:873	C57BL/6J mice	861:873	C57BL/6J mice fed a high fat diet to cause obesity (HFD)	861:916	DESIGN Cartilage damage was induced through cyclic compressive loading in four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT).
30240938	4	20	theme	mice	870:873	arg1	mice					640:643	adult male mice	629:643	adult male mice	629:643	DESIGN Cartilage damage was induced through cyclic compressive loading in four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT).
30240938	7	21	theme	TLR5KO	1216:1221	arg1	mice					1223:1226	TLR5KO mice	1216:1226	TLR5KO mice	1216:1226	After 6 weeks of loading, HFD mice had increased load-induced cartilage damage, while TLR5KO mice had cartilage damage comparable to WT mice.
30240938	4	22	theme	mice	686:689	arg1	mice					942:945	untreated C57BL/6J mice	923:945	untreated C57BL/6J mice (WT)	923:950	DESIGN Cartilage damage was induced through cyclic compressive loading in four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT).
30240938	4	22	theme	mice	686:689	arg1	mice					686:689	Toll-like receptor-5 deficient (TLR5KO) mice	646:689	Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome	646:762	DESIGN Cartilage damage was induced through cyclic compressive loading in four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT).
30240938	4	22	theme	mice	686:689	arg1	groups					619:624	four groups	614:624	four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT)	614:950	DESIGN Cartilage damage was induced through cyclic compressive loading in four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT).
30240938	4	22	theme	mice	686:689	arg1	WT					948:949	WT	948:949	WT	948:949	DESIGN Cartilage damage was induced through cyclic compressive loading in four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT).
30240938	4	22	theme	mice	686:689	arg1	mice					772:775	TLR5KO mice	765:775	TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota)	765:858	DESIGN Cartilage damage was induced through cyclic compressive loading in four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT).
30240938	4	22	theme	mice	686:689	arg1	mice					870:873	C57BL/6J mice	861:873	C57BL/6J mice fed a high fat diet to cause obesity (HFD)	861:916	DESIGN Cartilage damage was induced through cyclic compressive loading in four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT).
30240938	4	22	theme	mice	686:689	arg1	mice					640:643	adult male mice	629:643	adult male mice	629:643	DESIGN Cartilage damage was induced through cyclic compressive loading in four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT).
30240938	9	23	theme	inflammatory	1368:1379	arg1	markers					1381:1387	elevated serum inflammatory markers	1353:1387	elevated serum inflammatory markers	1353:1387	HFD mice had elevated serum inflammatory markers.
30240938	4	24	theme	Toll-like	646:654	arg1	mice					686:689	Toll-like receptor-5 deficient (TLR5KO) mice	646:689	Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome	646:762	DESIGN Cartilage damage was induced through cyclic compressive loading in four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT).
30240938	4	25	theme	deficient	667:675	arg1	mice					686:689	Toll-like receptor-5 deficient (TLR5KO) mice	646:689	Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome	646:762	DESIGN Cartilage damage was induced through cyclic compressive loading in four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT).
30240938	0	26	theme	gut	52:54	arg1	effects					4:10	The effects	0:10	The effects of metabolic syndrome, obesity, and the gut	0:54	The effects of metabolic syndrome, obesity, and the gut microbiome on load-induced osteoarthritis.
30240938	13	27	theme	TLR5KOΔMicrobiota	1758:1774	arg1	mice					1776:1779	the TLR5KOΔMicrobiota mice	1754:1779	the TLR5KOΔMicrobiota mice	1754:1779	Lastly, reduced cartilage damage in the TLR5KOΔMicrobiota mice suggests that the gut microbiome may influence cartilage pathology.
30240938	2	28	theme	syndrome	426:433	arg1	obesity					388:394	obesity	388:394	obesity	388:394	Metabolic syndrome is associated with osteoarthritis (OA), but it is unclear if the association is attributable to increased mechanical loading on joints caused by obesity or other aspects of metabolic syndrome.
30240938	2	28	theme	syndrome	426:433	arg1	aspects					405:411	other aspects	399:411	other aspects of metabolic syndrome	399:433	Metabolic syndrome is associated with osteoarthritis (OA), but it is unclear if the association is attributable to increased mechanical loading on joints caused by obesity or other aspects of metabolic syndrome.
30240938	7	29	theme	load-induced	1179:1190	arg1	damage					1202:1207	increased load-induced cartilage damage	1169:1207	increased load-induced cartilage damage	1169:1207	After 6 weeks of loading, HFD mice had increased load-induced cartilage damage, while TLR5KO mice had cartilage damage comparable to WT mice.
30240938	13	30	theme	cartilage	1734:1742	arg1	damage					1744:1749	reduced cartilage damage	1726:1749	reduced cartilage damage in the TLR5KOΔMicrobiota mice	1726:1779	Lastly, reduced cartilage damage in the TLR5KOΔMicrobiota mice suggests that the gut microbiome may influence cartilage pathology.
30240938	2	31	theme	other	399:403	arg1	aspects					405:411	other aspects	399:411	other aspects of metabolic syndrome	399:433	Metabolic syndrome is associated with osteoarthritis (OA), but it is unclear if the association is attributable to increased mechanical loading on joints caused by obesity or other aspects of metabolic syndrome.
30240938	4	32	theme	untreated	923:931	arg1	WT					948:949	WT	948:949	WT	948:949	DESIGN Cartilage damage was induced through cyclic compressive loading in four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT).
30240938	4	32	theme	untreated	923:931	arg1	mice					942:945	untreated C57BL/6J mice	923:945	untreated C57BL/6J mice (WT)	923:950	DESIGN Cartilage damage was induced through cyclic compressive loading in four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT).
30240938	4	33	theme	mice	772:775	arg1	mice					942:945	untreated C57BL/6J mice	923:945	untreated C57BL/6J mice (WT)	923:950	DESIGN Cartilage damage was induced through cyclic compressive loading in four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT).
30240938	4	33	theme	mice	772:775	arg1	mice					686:689	Toll-like receptor-5 deficient (TLR5KO) mice	646:689	Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome	646:762	DESIGN Cartilage damage was induced through cyclic compressive loading in four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT).
30240938	4	33	theme	mice	772:775	arg1	groups					619:624	four groups	614:624	four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT)	614:950	DESIGN Cartilage damage was induced through cyclic compressive loading in four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT).
30240938	4	33	theme	mice	772:775	arg1	WT					948:949	WT	948:949	WT	948:949	DESIGN Cartilage damage was induced through cyclic compressive loading in four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT).
30240938	4	33	theme	mice	772:775	arg1	mice					772:775	TLR5KO mice	765:775	TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota)	765:858	DESIGN Cartilage damage was induced through cyclic compressive loading in four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT).
30240938	4	33	theme	mice	772:775	arg1	mice					870:873	C57BL/6J mice	861:873	C57BL/6J mice fed a high fat diet to cause obesity (HFD)	861:916	DESIGN Cartilage damage was induced through cyclic compressive loading in four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT).
30240938	4	33	theme	mice	772:775	arg1	mice					640:643	adult male mice	629:643	adult male mice	629:643	DESIGN Cartilage damage was induced through cyclic compressive loading in four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT).
30240938	0	34	theme	syndrome	25:32	arg1	effects					4:10	The effects	0:10	The effects of metabolic syndrome, obesity, and the gut	0:54	The effects of metabolic syndrome, obesity, and the gut microbiome on load-induced osteoarthritis.
30240938	7	35	theme	HFD	1156:1158	arg1	mice					1160:1163	HFD mice	1156:1163	HFD mice	1156:1163	After 6 weeks of loading, HFD mice had increased load-induced cartilage damage, while TLR5KO mice had cartilage damage comparable to WT mice.
30240938	4	36	theme	mice	942:945	arg1	mice					942:945	untreated C57BL/6J mice	923:945	untreated C57BL/6J mice (WT)	923:950	DESIGN Cartilage damage was induced through cyclic compressive loading in four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT).
30240938	4	36	theme	mice	942:945	arg1	mice					686:689	Toll-like receptor-5 deficient (TLR5KO) mice	646:689	Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome	646:762	DESIGN Cartilage damage was induced through cyclic compressive loading in four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT).
30240938	4	36	theme	mice	942:945	arg1	groups					619:624	four groups	614:624	four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT)	614:950	DESIGN Cartilage damage was induced through cyclic compressive loading in four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT).
30240938	4	36	theme	mice	942:945	arg1	WT					948:949	WT	948:949	WT	948:949	DESIGN Cartilage damage was induced through cyclic compressive loading in four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT).
30240938	4	36	theme	mice	942:945	arg1	mice					772:775	TLR5KO mice	765:775	TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota)	765:858	DESIGN Cartilage damage was induced through cyclic compressive loading in four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT).
30240938	4	36	theme	mice	942:945	arg1	mice					870:873	C57BL/6J mice	861:873	C57BL/6J mice fed a high fat diet to cause obesity (HFD)	861:916	DESIGN Cartilage damage was induced through cyclic compressive loading in four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT).
30240938	4	36	theme	mice	942:945	arg1	mice					640:643	adult male mice	629:643	adult male mice	629:643	DESIGN Cartilage damage was induced through cyclic compressive loading in four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT).
30240938	11	37	theme	load-induced	1481:1492	arg1	damage					1504:1509	load-induced cartilage damage	1481:1509	load-induced cartilage damage	1481:1509	CONCLUSIONS Severe obesity increased load-induced cartilage damage, while milder changes in adiposity/metabolic syndrome seen in TLR5KO mice did not.
30240938	0	38	theme	obesity	35:41	arg1	effects					4:10	The effects	0:10	The effects of metabolic syndrome, obesity, and the gut	0:54	The effects of metabolic syndrome, obesity, and the gut microbiome on load-induced osteoarthritis.
30240938	7	39	theme	loading	1147:1153	arg1	weeks					1138:1142	6 weeks	1136:1142	6 weeks of loading	1136:1153	After 6 weeks of loading, HFD mice had increased load-induced cartilage damage, while TLR5KO mice had cartilage damage comparable to WT mice.
30240938	4	40	theme	gut	749:751	arg1	microbiome					753:762	the gut microbiome	745:762	the gut microbiome	745:762	DESIGN Cartilage damage was induced through cyclic compressive loading in four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT).
30240938	10	41	theme	gut	1416:1418	arg1	composition					1431:1441	a distinct gut microbiome composition	1405:1441	a distinct gut microbiome composition	1405:1441	Each group had a distinct gut microbiome composition.
30240938	2	42	theme	mechanical	349:358	arg1	loading					360:366	increased mechanical loading	339:366	increased mechanical loading on joints caused by obesity or other aspects of metabolic syndrome	339:433	Metabolic syndrome is associated with osteoarthritis (OA), but it is unclear if the association is attributable to increased mechanical loading on joints caused by obesity or other aspects of metabolic syndrome.
30240938	12	43	theme	mechanical	1698:1707	arg1	loading					1709:1715	mechanical loading	1698:1715	mechanical loading	1698:1715	Furthermore, the effects of systemic inflammation/obesity on cartilage damage depend on the duration of mechanical loading.
30240938	4	44	theme	cyclic	584:589	arg1	loading					603:609	cyclic compressive loading	584:609	cyclic compressive loading	584:609	DESIGN Cartilage damage was induced through cyclic compressive loading in four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT).
30240938	11	45	theme	adiposity/metabolic	1536:1554	arg1	syndrome					1556:1563	adiposity/metabolic syndrome	1536:1563	adiposity/metabolic syndrome seen in TLR5KO mice	1536:1583	CONCLUSIONS Severe obesity increased load-induced cartilage damage, while milder changes in adiposity/metabolic syndrome seen in TLR5KO mice did not.
30240938	3	46	theme	metabolism	476:485	arg1	effects					457:463	the effects	453:463	the effects of altered metabolism, obesity, and the gut microbiome on load-induced OA	453:537	Here we examined the effects of altered metabolism, obesity, and the gut microbiome on load-induced OA.
30240938	4	47	theme	metabolic	821:829	arg1	syndrome					831:838	metabolic syndrome	821:838	metabolic syndrome (TLR5KOΔMicrobiota)	821:858	DESIGN Cartilage damage was induced through cyclic compressive loading in four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT).
30240938	4	47	theme	metabolic	821:829	arg1	TLR5KOΔMicrobiota					841:857	TLR5KOΔMicrobiota	841:857	TLR5KOΔMicrobiota	841:857	DESIGN Cartilage damage was induced through cyclic compressive loading in four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT).
30240938	4	48	theme	DESIGN	540:545	arg1	damage					557:562	DESIGN Cartilage damage	540:562	DESIGN Cartilage damage	540:562	DESIGN Cartilage damage was induced through cyclic compressive loading in four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT).
30240938	11	49	theme	CONCLUSIONS	1444:1454	arg1	obesity					1463:1469	CONCLUSIONS Severe obesity	1444:1469	CONCLUSIONS Severe obesity	1444:1469	CONCLUSIONS Severe obesity increased load-induced cartilage damage, while milder changes in adiposity/metabolic syndrome seen in TLR5KO mice did not.
30240938	3	50	theme	gut	505:507	arg1	microbiome					509:518	the gut microbiome	501:518	the gut microbiome	501:518	Here we examined the effects of altered metabolism, obesity, and the gut microbiome on load-induced OA.
30240938	2	51	from	loading	360:366	arg1	joints					371:376	joints	371:376	joints caused by obesity or other aspects of metabolic syndrome	371:433	Metabolic syndrome is associated with osteoarthritis (OA), but it is unclear if the association is attributable to increased mechanical loading on joints caused by obesity or other aspects of metabolic syndrome.
30240938	11	52	theme	TLR5KO	1573:1578	arg1	mice					1580:1583	TLR5KO mice	1573:1583	TLR5KO mice	1573:1583	CONCLUSIONS Severe obesity increased load-induced cartilage damage, while milder changes in adiposity/metabolic syndrome seen in TLR5KO mice did not.
30240938	4	53	theme	chronic	790:796	arg1	antibiotics					798:808	chronic antibiotics	790:808	chronic antibiotics	790:808	DESIGN Cartilage damage was induced through cyclic compressive loading in four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT).
30240938	12	54	theme	systemic	1622:1629	arg1	inflammation/obesity					1631:1650	systemic inflammation/obesity	1622:1650	systemic inflammation/obesity	1622:1650	Furthermore, the effects of systemic inflammation/obesity on cartilage damage depend on the duration of mechanical loading.
30240938	3	55	from	effects	457:463	arg1	OA					536:537	load-induced OA	523:537	load-induced OA	523:537	Here we examined the effects of altered metabolism, obesity, and the gut microbiome on load-induced OA.
30240938	4	56	theme	male	635:638	arg1	mice					640:643	adult male mice	629:643	adult male mice	629:643	DESIGN Cartilage damage was induced through cyclic compressive loading in four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT).
30240938	1	57	theme	insulin	186:192	arg1	resistance					194:203	insulin resistance	186:203	insulin resistance	186:203	OBJECTIVE Metabolic syndrome is characterized by obesity, hyperglycemia, hypertension, insulin resistance, and dyslipidemia.
30240938	7	58	theme	WT	1263:1264	arg1	mice					1266:1269	WT mice	1263:1269	WT mice	1263:1269	After 6 weeks of loading, HFD mice had increased load-induced cartilage damage, while TLR5KO mice had cartilage damage comparable to WT mice.
30240938	9	59	theme	HFD	1340:1342	arg1	mice					1344:1347	HFD mice	1340:1347	HFD mice	1340:1347	HFD mice had elevated serum inflammatory markers.
30240938	4	60	theme	high	881:884	arg1	HFD					913:915	HFD	913:915	HFD	913:915	DESIGN Cartilage damage was induced through cyclic compressive loading in four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT).
30240938	4	60	theme	high	881:884	arg1	diet					890:893	a high fat diet	879:893	a high fat diet to cause obesity (HFD)	879:916	DESIGN Cartilage damage was induced through cyclic compressive loading in four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT).
30240938	8	61	theme	other	1326:1330	arg1	groups					1332:1337	other groups	1326:1337	other groups	1326:1337	TLR5KOΔMicrobiota mice had less cartilage damage than other groups.
30240938	7	62	theme	comparable	1249:1258	arg1	damage					1242:1247	cartilage damage	1232:1247	cartilage damage comparable to WT mice	1232:1269	After 6 weeks of loading, HFD mice had increased load-induced cartilage damage, while TLR5KO mice had cartilage damage comparable to WT mice.
30240938	1	63	theme	OBJECTIVE	99:107	arg1	syndrome					119:126	OBJECTIVE Metabolic syndrome	99:126	OBJECTIVE Metabolic syndrome	99:126	OBJECTIVE Metabolic syndrome is characterized by obesity, hyperglycemia, hypertension, insulin resistance, and dyslipidemia.
30240938	7	64	theme	cartilage	1232:1240	arg1	damage					1242:1247	cartilage damage	1232:1247	cartilage damage comparable to WT mice	1232:1269	After 6 weeks of loading, HFD mice had increased load-induced cartilage damage, while TLR5KO mice had cartilage damage comparable to WT mice.
30240938	9	65	theme	serum	1362:1366	arg1	markers					1381:1387	elevated serum inflammatory markers	1353:1387	elevated serum inflammatory markers	1353:1387	HFD mice had elevated serum inflammatory markers.
30240938	4	66	theme	C57BL/6J	861:868	arg1	mice					870:873	C57BL/6J mice	861:873	C57BL/6J mice fed a high fat diet to cause obesity (HFD)	861:916	DESIGN Cartilage damage was induced through cyclic compressive loading in four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT).
30240938	6	67	theme	OARSI	1085:1089	arg1	damage					1077:1082	cartilage damage	1067:1082	cartilage damage (OARSI score)	1067:1096	RESULTS After 2 weeks of loading, cartilage damage (OARSI score) was not different among groups.
30240938	6	67	theme	OARSI	1085:1089	arg1	score					1091:1095	OARSI score	1085:1095	OARSI score	1085:1095	RESULTS After 2 weeks of loading, cartilage damage (OARSI score) was not different among groups.
30240938	13	68	theme	cartilage	1828:1836	arg1	pathology					1838:1846	cartilage pathology	1828:1846	cartilage pathology	1828:1846	Lastly, reduced cartilage damage in the TLR5KOΔMicrobiota mice suggests that the gut microbiome may influence cartilage pathology.
30240938	4	69	dep	deficient	667:675	arg1	TLR5KO					678:683	TLR5KO	678:683	TLR5KO	678:683	DESIGN Cartilage damage was induced through cyclic compressive loading in four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT).
30240938	13	70	theme	gut	1799:1801	arg1	microbiome					1803:1812	the gut microbiome	1795:1812	the gut microbiome	1795:1812	Lastly, reduced cartilage damage in the TLR5KOΔMicrobiota mice suggests that the gut microbiome may influence cartilage pathology.
30240938	0	71	theme	load-induced	70:81	arg1	osteoarthritis					83:96	load-induced osteoarthritis	70:96	load-induced osteoarthritis	70:96	The effects of metabolic syndrome, obesity, and the gut microbiome on load-induced osteoarthritis.
30240938	4	72	from	alterations	730:740	arg1	microbiome					753:762	the gut microbiome	745:762	the gut microbiome	745:762	DESIGN Cartilage damage was induced through cyclic compressive loading in four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT).
30240938	10	73	contain	had	1401:1403	arg2	composition					1431:1441	a distinct gut microbiome composition	1405:1441	a distinct gut microbiome composition	1405:1441	Each group had a distinct gut microbiome composition.
30240938	10	73	contain	had	1401:1403	arg1	group					1395:1399	Each group	1390:1399	Each group	1390:1399	Each group had a distinct gut microbiome composition.
30240938	4	74	theme	due	723:725	arg1	syndrome					714:721	metabolic syndrome	704:721	metabolic syndrome due to alterations in the gut microbiome	704:762	DESIGN Cartilage damage was induced through cyclic compressive loading in four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT).
30240938	7	75	theme	cartilage	1192:1200	arg1	damage					1202:1207	increased load-induced cartilage damage	1169:1207	increased load-induced cartilage damage	1169:1207	After 6 weeks of loading, HFD mice had increased load-induced cartilage damage, while TLR5KO mice had cartilage damage comparable to WT mice.
30240938	7	76	theme	increased	1169:1177	arg1	damage					1202:1207	increased load-induced cartilage damage	1169:1207	increased load-induced cartilage damage	1169:1207	After 6 weeks of loading, HFD mice had increased load-induced cartilage damage, while TLR5KO mice had cartilage damage comparable to WT mice.
30240938	2	77	theme	metabolic	416:424	arg1	syndrome					426:433	metabolic syndrome	416:433	metabolic syndrome	416:433	Metabolic syndrome is associated with osteoarthritis (OA), but it is unclear if the association is attributable to increased mechanical loading on joints caused by obesity or other aspects of metabolic syndrome.
30240938	13	78	theme	reduced	1726:1732	arg1	damage					1744:1749	reduced cartilage damage	1726:1749	reduced cartilage damage in the TLR5KOΔMicrobiota mice	1726:1779	Lastly, reduced cartilage damage in the TLR5KOΔMicrobiota mice suggests that the gut microbiome may influence cartilage pathology.
30240938	0	79	theme	metabolic	15:23	arg1	syndrome					25:32	metabolic syndrome	15:32	metabolic syndrome	15:32	The effects of metabolic syndrome, obesity, and the gut microbiome on load-induced osteoarthritis.
30240938	4	80	theme	metabolic	704:712	arg1	syndrome					714:721	metabolic syndrome	704:721	metabolic syndrome due to alterations in the gut microbiome	704:762	DESIGN Cartilage damage was induced through cyclic compressive loading in four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT).
30240938	3	81	theme	obesity	488:494	arg1	effects					457:463	the effects	453:463	the effects of altered metabolism, obesity, and the gut microbiome on load-induced OA	453:537	Here we examined the effects of altered metabolism, obesity, and the gut microbiome on load-induced OA.
30240938	13	82	from	damage	1744:1749	arg1	mice					1776:1779	the TLR5KOΔMicrobiota mice	1754:1779	the TLR5KOΔMicrobiota mice	1754:1779	Lastly, reduced cartilage damage in the TLR5KOΔMicrobiota mice suggests that the gut microbiome may influence cartilage pathology.
30240938	11	83	from	changes	1525:1531	arg1	syndrome					1556:1563	adiposity/metabolic syndrome	1536:1563	adiposity/metabolic syndrome seen in TLR5KO mice	1536:1583	CONCLUSIONS Severe obesity increased load-induced cartilage damage, while milder changes in adiposity/metabolic syndrome seen in TLR5KO mice did not.
30240938	9	84	contain	had	1349:1351	arg2	markers					1381:1387	elevated serum inflammatory markers	1353:1387	elevated serum inflammatory markers	1353:1387	HFD mice had elevated serum inflammatory markers.
30240938	9	84	contain	had	1349:1351	arg1	mice					1344:1347	HFD mice	1340:1347	HFD mice	1340:1347	HFD mice had elevated serum inflammatory markers.
30240938	4	85	theme	TLR5KO	765:770	arg1	mice					772:775	TLR5KO mice	765:775	TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota)	765:858	DESIGN Cartilage damage was induced through cyclic compressive loading in four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT).
30240938	12	86	theme	loading	1709:1715	arg1	duration					1686:1693	the duration	1682:1693	the duration of mechanical loading	1682:1715	Furthermore, the effects of systemic inflammation/obesity on cartilage damage depend on the duration of mechanical loading.
30240938	4	87	theme	C57BL/6J	933:940	arg1	WT					948:949	WT	948:949	WT	948:949	DESIGN Cartilage damage was induced through cyclic compressive loading in four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT).
30240938	4	87	theme	C57BL/6J	933:940	arg1	mice					942:945	untreated C57BL/6J mice	923:945	untreated C57BL/6J mice (WT)	923:950	DESIGN Cartilage damage was induced through cyclic compressive loading in four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT).
30240938	11	88	theme	Severe	1456:1461	arg1	obesity					1463:1469	CONCLUSIONS Severe obesity	1444:1469	CONCLUSIONS Severe obesity	1444:1469	CONCLUSIONS Severe obesity increased load-induced cartilage damage, while milder changes in adiposity/metabolic syndrome seen in TLR5KO mice did not.
30240938	3	89	theme	altered	468:474	arg1	metabolism					476:485	altered metabolism	468:485	altered metabolism	468:485	Here we examined the effects of altered metabolism, obesity, and the gut microbiome on load-induced OA.
30240938	8	90	theme	TLR5KOΔMicrobiota	1272:1288	arg1	mice					1290:1293	TLR5KOΔMicrobiota mice	1272:1293	TLR5KOΔMicrobiota mice	1272:1293	TLR5KOΔMicrobiota mice had less cartilage damage than other groups.
30240938	2	91	theme	increased	339:347	arg1	loading					360:366	increased mechanical loading	339:366	increased mechanical loading on joints caused by obesity or other aspects of metabolic syndrome	339:433	Metabolic syndrome is associated with osteoarthritis (OA), but it is unclear if the association is attributable to increased mechanical loading on joints caused by obesity or other aspects of metabolic syndrome.
30240938	4	92	theme	compressive	591:601	arg1	loading					603:609	cyclic compressive loading	584:609	cyclic compressive loading	584:609	DESIGN Cartilage damage was induced through cyclic compressive loading in four groups of adult male mice: Toll-like receptor-5 deficient (TLR5KO) mice that develop metabolic syndrome due to alterations in the gut microbiome, TLR5KO mice submitted to chronic antibiotics to prevent metabolic syndrome (TLR5KOΔMicrobiota), C57BL/6J mice fed a high fat diet to cause obesity (HFD), and untreated C57BL/6J mice (WT).
30240938	10	93	theme	distinct	1407:1414	arg1	composition					1431:1441	a distinct gut microbiome composition	1405:1441	a distinct gut microbiome composition	1405:1441	Each group had a distinct gut microbiome composition.
30240938	11	94	theme	cartilage	1494:1502	arg1	damage					1504:1509	load-induced cartilage damage	1481:1509	load-induced cartilage damage	1481:1509	CONCLUSIONS Severe obesity increased load-induced cartilage damage, while milder changes in adiposity/metabolic syndrome seen in TLR5KO mice did not.
30240938	8	95	contain	had	1295:1297	arg1	mice					1290:1293	TLR5KOΔMicrobiota mice	1272:1293	TLR5KOΔMicrobiota mice	1272:1293	TLR5KOΔMicrobiota mice had less cartilage damage than other groups.
30240938	8	95	contain	had	1295:1297	arg2	damage					1314:1319	less cartilage damage	1299:1319	less cartilage damage	1299:1319	TLR5KOΔMicrobiota mice had less cartilage damage than other groups.
30479660	0	0	theme	cytoplasmic	93:103	arg1	proteins					105:112	cytoplasmic proteins	93:112	cytoplasmic proteins	93:112	Clostridium acetobutylicum grows vegetatively in a biofilm rich in heteropolysaccharides and cytoplasmic proteins.
30479660	11	1	theme	acetobutylicum	1416:1429	arg1	cells					1431:1435	vegetative C. acetobutylicum cells	1402:1435	vegetative C. acetobutylicum cells rather than commonly assumed spore-forming cells	1402:1484	CONCLUSIONS This study evidenced that vegetative C. acetobutylicum cells rather than commonly assumed spore-forming cells were essentially the solvent-forming cells.
30479660	13	2	theme	processes	1814:1822	arg1	development					1781:1791	development	1781:1791	development	1781:1791	This study provides the first physiological and molecular insights into C. acetobutylicum biofilm which should be valuable for understanding and development of the biofilm-based processes.
30479660	13	2	theme	processes	1814:1822	arg1	understanding					1763:1775	understanding	1763:1775	understanding	1763:1775	This study provides the first physiological and molecular insights into C. acetobutylicum biofilm which should be valuable for understanding and development of the biofilm-based processes.
30479660	3	3	from	physiology	460:469	arg1	biofilm					495:501	biofilm	495:501	biofilm	495:501	However, little has been known about the physiology of C. acetobutylicum in biofilm as well as the composition and biosynthesis of the EPS.
30479660	2	4	theme	liquid	383:388	arg1	biofuels					390:397	liquid biofuels	383:397	liquid biofuels	383:397	The biofilm of Clostridium acetobutylicum confers the cells superior phenotypes and has been extensively exploited to produce a variety of liquid biofuels and bulk chemicals.
30479660	10	5	theme	great	1139:1143	arg1	proteins					1156:1163	proteins	1156:1163	proteins including many non-classically secreted proteins moonlighting as adhesins	1156:1237	Also, a great variety of proteins including many non-classically secreted proteins moonlighting as adhesins were found considerably present in the biofilm, with GroEL, a S-layer protein and rubrerythrin being the most abundant ones.
30479660	10	5	theme	great	1139:1143	arg1	variety					1145:1151	a great variety	1137:1151	a great variety of proteins including many non-classically secreted proteins moonlighting as adhesins	1137:1237	Also, a great variety of proteins including many non-classically secreted proteins moonlighting as adhesins were found considerably present in the biofilm, with GroEL, a S-layer protein and rubrerythrin being the most abundant ones.
30479660	10	5	theme	great	1139:1143	arg1	proteins					1205:1212	many non-classically secreted proteins	1175:1212	many non-classically secreted proteins moonlighting as adhesins	1175:1237	Also, a great variety of proteins including many non-classically secreted proteins moonlighting as adhesins were found considerably present in the biofilm, with GroEL, a S-layer protein and rubrerythrin being the most abundant ones.
30479660	5	6	from	lifestyle	704:712	arg1	biofilm					738:744	biofilm	738:744	biofilm	738:744	RESULTS Here, we revealed a novel lifestyle of C. acetobutylicum in biofilm: elimination of sporulation and vegetative growth.
30479660	5	7	theme	sporulation	762:772	arg1	elimination					747:757	elimination	747:757	elimination of sporulation and vegetative growth	747:794	RESULTS Here, we revealed a novel lifestyle of C. acetobutylicum in biofilm: elimination of sporulation and vegetative growth.
30479660	12	8	theme	abundant	1534:1541	arg1	proteins					1581:1588	The abundant non-classically secreted moonlighting proteins	1530:1588	The abundant non-classically secreted moonlighting proteins	1530:1588	The abundant non-classically secreted moonlighting proteins might be important for the biofilm formation.
30479660	12	8	theme	abundant	1534:1541	arg1	important					1599:1607	important	1599:1607	important	1599:1607	The abundant non-classically secreted moonlighting proteins might be important for the biofilm formation.
30479660	11	9	dep	CONCLUSIONS	1364:1374	arg1	evidenced					1387:1395	evidenced	1387:1395	evidenced that vegetative C. acetobutylicum cells rather than commonly assumed spore-forming cells	1387:1484	CONCLUSIONS This study evidenced that vegetative C. acetobutylicum cells rather than commonly assumed spore-forming cells were essentially the solvent-forming cells.
30479660	1	10	from	embedded	174:181	arg1	EPS					238:240	EPS	238:240	EPS	238:240	BACKGROUND Biofilms are cell communities wherein cells are embedded in a self-produced extracellular polymeric substances (EPS).
30479660	1	10	from	embedded	174:181	arg1	substances					226:235	a self-produced extracellular polymeric substances	186:235	a self-produced extracellular polymeric substances (EPS)	186:241	BACKGROUND Biofilms are cell communities wherein cells are embedded in a self-produced extracellular polymeric substances (EPS).
30479660	13	11	theme	biofilm-based	1800:1812	arg1	processes					1814:1822	the biofilm-based processes	1796:1822	the biofilm-based processes	1796:1822	This study provides the first physiological and molecular insights into C. acetobutylicum biofilm which should be valuable for understanding and development of the biofilm-based processes.
30479660	11	12	theme	spore-forming	1466:1478	arg1	cells					1480:1484	commonly assumed spore-forming cells	1449:1484	vegetative C. acetobutylicum cells rather than commonly assumed spore-forming cells	1402:1484	CONCLUSIONS This study evidenced that vegetative C. acetobutylicum cells rather than commonly assumed spore-forming cells were essentially the solvent-forming cells.
30479660	3	13	from	composition	518:528	arg1	biofilm					495:501	biofilm	495:501	biofilm	495:501	However, little has been known about the physiology of C. acetobutylicum in biofilm as well as the composition and biosynthesis of the EPS.
30479660	3	14	theme	EPS	554:556	arg1	composition					518:528	composition	518:528	composition	518:528	However, little has been known about the physiology of C. acetobutylicum in biofilm as well as the composition and biosynthesis of the EPS.
30479660	3	14	theme	EPS	554:556	arg1	physiology					460:469	the physiology	456:469	the physiology of C. acetobutylicum in biofilm as well as the composition and biosynthesis of the EPS	456:556	However, little has been known about the physiology of C. acetobutylicum in biofilm as well as the composition and biosynthesis of the EPS.
30479660	3	14	theme	EPS	554:556	arg1	biosynthesis					534:545	biosynthesis	534:545	biosynthesis	534:545	However, little has been known about the physiology of C. acetobutylicum in biofilm as well as the composition and biosynthesis of the EPS.
30479660	5	15	theme	acetobutylicum	720:733	arg1	lifestyle					704:712	a novel lifestyle	696:712	a novel lifestyle of C. acetobutylicum in biofilm	696:744	RESULTS Here, we revealed a novel lifestyle of C. acetobutylicum in biofilm: elimination of sporulation and vegetative growth.
30479660	0	16	from	rich	59:62	arg1	heteropolysaccharides					67:87	heteropolysaccharides	67:87	heteropolysaccharides	67:87	Clostridium acetobutylicum grows vegetatively in a biofilm rich in heteropolysaccharides and cytoplasmic proteins.
30479660	0	16	from	rich	59:62	arg1	proteins					105:112	cytoplasmic proteins	93:112	cytoplasmic proteins	93:112	Clostridium acetobutylicum grows vegetatively in a biofilm rich in heteropolysaccharides and cytoplasmic proteins.
30479660	6	17	theme	polymeric	811:819	arg1	substances					821:830	Extracellular polymeric substances	797:830	Extracellular polymeric substances	797:830	Extracellular polymeric substances and wire-like structures were also observed in the biofilm.
30479660	6	18	located	observed	867:874	arg2	substances					821:830	Extracellular polymeric substances	797:830	Extracellular polymeric substances	797:830	Extracellular polymeric substances and wire-like structures were also observed in the biofilm.
30479660	6	18	located	observed	867:874	arg2	structures					846:855	wire-like structures	836:855	wire-like structures	836:855	Extracellular polymeric substances and wire-like structures were also observed in the biofilm.
30479660	6	18	located	observed	867:874	arg1	biofilm					883:889	the biofilm	879:889	the biofilm	879:889	Extracellular polymeric substances and wire-like structures were also observed in the biofilm.
30479660	3	19	from	biosynthesis	534:545	arg1	biofilm					495:501	biofilm	495:501	biofilm	495:501	However, little has been known about the physiology of C. acetobutylicum in biofilm as well as the composition and biosynthesis of the EPS.
30479660	2	20	theme	superior	304:311	arg1	phenotypes					313:322	the cells superior phenotypes	294:322	the cells superior phenotypes	294:322	The biofilm of Clostridium acetobutylicum confers the cells superior phenotypes and has been extensively exploited to produce a variety of liquid biofuels and bulk chemicals.
30479660	1	21	theme	extracellular	202:214	arg1	EPS					238:240	EPS	238:240	EPS	238:240	BACKGROUND Biofilms are cell communities wherein cells are embedded in a self-produced extracellular polymeric substances (EPS).
30479660	1	21	theme	extracellular	202:214	arg1	substances					226:235	a self-produced extracellular polymeric substances	186:235	a self-produced extracellular polymeric substances (EPS)	186:241	BACKGROUND Biofilms are cell communities wherein cells are embedded in a self-produced extracellular polymeric substances (EPS).
30479660	4	22	theme	biofilm	661:667	arg1	physiology					609:618	cell physiology	604:618	cell physiology	604:618	Thus, this study is focused on revealing the cell physiology and EPS composition of C. acetobutylicum biofilm.
30479660	4	22	theme	biofilm	661:667	arg1	composition					628:638	EPS composition	624:638	EPS composition	624:638	Thus, this study is focused on revealing the cell physiology and EPS composition of C. acetobutylicum biofilm.
30479660	11	23	theme	assumed	1458:1464	arg1	cells					1480:1484	commonly assumed spore-forming cells	1449:1484	vegetative C. acetobutylicum cells rather than commonly assumed spore-forming cells	1402:1484	CONCLUSIONS This study evidenced that vegetative C. acetobutylicum cells rather than commonly assumed spore-forming cells were essentially the solvent-forming cells.
30479660	12	24	theme	biofilm	1617:1623	arg1	formation					1625:1633	the biofilm formation	1613:1633	the biofilm formation	1613:1633	The abundant non-classically secreted moonlighting proteins might be important for the biofilm formation.
30479660	2	25	theme	cells	298:302	arg1	phenotypes					313:322	the cells superior phenotypes	294:322	the cells superior phenotypes	294:322	The biofilm of Clostridium acetobutylicum confers the cells superior phenotypes and has been extensively exploited to produce a variety of liquid biofuels and bulk chemicals.
30479660	1	26	theme	self-produced	188:200	arg1	EPS					238:240	EPS	238:240	EPS	238:240	BACKGROUND Biofilms are cell communities wherein cells are embedded in a self-produced extracellular polymeric substances (EPS).
30479660	1	26	theme	self-produced	188:200	arg1	substances					226:235	a self-produced extracellular polymeric substances	186:235	a self-produced extracellular polymeric substances (EPS)	186:241	BACKGROUND Biofilms are cell communities wherein cells are embedded in a self-produced extracellular polymeric substances (EPS).
30479660	13	27	theme	first	1660:1664	arg1	insights					1694:1701	the first physiological and molecular insights	1656:1701	the first physiological and molecular insights into C. acetobutylicum biofilm which should be valuable for understanding and development of the biofilm-based processes	1656:1822	This study provides the first physiological and molecular insights into C. acetobutylicum biofilm which should be valuable for understanding and development of the biofilm-based processes.
30479660	13	28	theme	C.	1708:1709	arg1	biofilm					1726:1732	C. acetobutylicum biofilm	1708:1732	C. acetobutylicum biofilm which should be valuable for understanding and development of the biofilm-based processes	1708:1822	This study provides the first physiological and molecular insights into C. acetobutylicum biofilm which should be valuable for understanding and development of the biofilm-based processes.
30479660	2	29	theme	chemicals	408:416	arg1	variety					372:378	a variety	370:378	a variety of liquid biofuels and bulk chemicals	370:416	The biofilm of Clostridium acetobutylicum confers the cells superior phenotypes and has been extensively exploited to produce a variety of liquid biofuels and bulk chemicals.
30479660	2	29	theme	chemicals	408:416	arg1	biofuels					390:397	liquid biofuels	383:397	liquid biofuels	383:397	The biofilm of Clostridium acetobutylicum confers the cells superior phenotypes and has been extensively exploited to produce a variety of liquid biofuels and bulk chemicals.
30479660	2	29	theme	chemicals	408:416	arg1	chemicals					408:416	bulk chemicals	403:416	bulk chemicals	403:416	The biofilm of Clostridium acetobutylicum confers the cells superior phenotypes and has been extensively exploited to produce a variety of liquid biofuels and bulk chemicals.
30479660	0	30	theme	Clostridium	0:10	arg1	acetobutylicum					12:25	Clostridium acetobutylicum	0:25	Clostridium acetobutylicum	0:25	Clostridium acetobutylicum grows vegetatively in a biofilm rich in heteropolysaccharides and cytoplasmic proteins.
30479660	6	31	theme	wire-like	836:844	arg1	structures					846:855	wire-like structures	836:855	wire-like structures	836:855	Extracellular polymeric substances and wire-like structures were also observed in the biofilm.
30479660	3	32	dep	composition	518:528	arg1	the					514:516	the	514:516	the	514:516	However, little has been known about the physiology of C. acetobutylicum in biofilm as well as the composition and biosynthesis of the EPS.
30479660	2	33	theme	bulk	403:406	arg1	chemicals					408:416	bulk chemicals	403:416	bulk chemicals	403:416	The biofilm of Clostridium acetobutylicum confers the cells superior phenotypes and has been extensively exploited to produce a variety of liquid biofuels and bulk chemicals.
30479660	10	34	theme	proteins	1156:1163	arg1	proteins					1156:1163	proteins	1156:1163	proteins including many non-classically secreted proteins moonlighting as adhesins	1156:1237	Also, a great variety of proteins including many non-classically secreted proteins moonlighting as adhesins were found considerably present in the biofilm, with GroEL, a S-layer protein and rubrerythrin being the most abundant ones.
30479660	10	34	theme	proteins	1156:1163	arg1	variety					1145:1151	a great variety	1137:1151	a great variety of proteins including many non-classically secreted proteins moonlighting as adhesins	1137:1237	Also, a great variety of proteins including many non-classically secreted proteins moonlighting as adhesins were found considerably present in the biofilm, with GroEL, a S-layer protein and rubrerythrin being the most abundant ones.
30479660	10	34	theme	proteins	1156:1163	arg1	proteins					1205:1212	many non-classically secreted proteins	1175:1212	many non-classically secreted proteins moonlighting as adhesins	1175:1237	Also, a great variety of proteins including many non-classically secreted proteins moonlighting as adhesins were found considerably present in the biofilm, with GroEL, a S-layer protein and rubrerythrin being the most abundant ones.
30479660	10	35	with	biofilm	1278:1284	arg1	rubrerythrin					1321:1332	rubrerythrin	1321:1332	rubrerythrin	1321:1332	Also, a great variety of proteins including many non-classically secreted proteins moonlighting as adhesins were found considerably present in the biofilm, with GroEL, a S-layer protein and rubrerythrin being the most abundant ones.
30479660	10	35	with	biofilm	1278:1284	arg1	protein					1309:1315	a S-layer protein	1299:1315	a S-layer protein	1299:1315	Also, a great variety of proteins including many non-classically secreted proteins moonlighting as adhesins were found considerably present in the biofilm, with GroEL, a S-layer protein and rubrerythrin being the most abundant ones.
30479660	10	35	with	biofilm	1278:1284	arg1	GroEL					1292:1296	GroEL	1292:1296	GroEL	1292:1296	Also, a great variety of proteins including many non-classically secreted proteins moonlighting as adhesins were found considerably present in the biofilm, with GroEL, a S-layer protein and rubrerythrin being the most abundant ones.
30479660	6	36	theme	Extracellular	797:809	arg1	substances					821:830	Extracellular polymeric substances	797:830	Extracellular polymeric substances	797:830	Extracellular polymeric substances and wire-like structures were also observed in the biofilm.
30479660	1	37	theme	BACKGROUND	115:124	arg1	communities					144:154	cell communities	139:154	cell communities	139:154	BACKGROUND Biofilms are cell communities wherein cells are embedded in a self-produced extracellular polymeric substances (EPS).
30479660	1	37	theme	BACKGROUND	115:124	arg1	Biofilms					126:133	BACKGROUND Biofilms	115:133	BACKGROUND Biofilms	115:133	BACKGROUND Biofilms are cell communities wherein cells are embedded in a self-produced extracellular polymeric substances (EPS).
30479660	10	38	theme	abundant	1349:1356	arg1	ones					1358:1361	the most abundant ones	1340:1361	the most abundant ones	1340:1361	Also, a great variety of proteins including many non-classically secreted proteins moonlighting as adhesins were found considerably present in the biofilm, with GroEL, a S-layer protein and rubrerythrin being the most abundant ones.
30479660	3	39	theme	acetobutylicum	477:490	arg1	composition					518:528	composition	518:528	composition	518:528	However, little has been known about the physiology of C. acetobutylicum in biofilm as well as the composition and biosynthesis of the EPS.
30479660	3	39	theme	acetobutylicum	477:490	arg1	physiology					460:469	the physiology	456:469	the physiology of C. acetobutylicum in biofilm as well as the composition and biosynthesis of the EPS	456:556	However, little has been known about the physiology of C. acetobutylicum in biofilm as well as the composition and biosynthesis of the EPS.
30479660	3	39	theme	acetobutylicum	477:490	arg1	biosynthesis					534:545	biosynthesis	534:545	biosynthesis	534:545	However, little has been known about the physiology of C. acetobutylicum in biofilm as well as the composition and biosynthesis of the EPS.
30479660	13	40	theme	physiological	1666:1678	arg1	insights					1694:1701	the first physiological and molecular insights	1656:1701	the first physiological and molecular insights into C. acetobutylicum biofilm which should be valuable for understanding and development of the biofilm-based processes	1656:1822	This study provides the first physiological and molecular insights into C. acetobutylicum biofilm which should be valuable for understanding and development of the biofilm-based processes.
30479660	5	41	theme	novel	698:702	arg1	lifestyle					704:712	a novel lifestyle	696:712	a novel lifestyle of C. acetobutylicum in biofilm	696:744	RESULTS Here, we revealed a novel lifestyle of C. acetobutylicum in biofilm: elimination of sporulation and vegetative growth.
30479660	13	42	theme	molecular	1684:1692	arg1	insights					1694:1701	the first physiological and molecular insights	1656:1701	the first physiological and molecular insights into C. acetobutylicum biofilm which should be valuable for understanding and development of the biofilm-based processes	1656:1822	This study provides the first physiological and molecular insights into C. acetobutylicum biofilm which should be valuable for understanding and development of the biofilm-based processes.
30479660	4	43	theme	EPS	624:626	arg1	composition					628:638	EPS composition	624:638	EPS composition	624:638	Thus, this study is focused on revealing the cell physiology and EPS composition of C. acetobutylicum biofilm.
30479660	11	44	theme	vegetative	1402:1411	arg1	cells					1431:1435	vegetative C. acetobutylicum cells	1402:1435	vegetative C. acetobutylicum cells rather than commonly assumed spore-forming cells	1402:1484	CONCLUSIONS This study evidenced that vegetative C. acetobutylicum cells rather than commonly assumed spore-forming cells were essentially the solvent-forming cells.
30479660	11	45	theme	solvent-forming	1507:1521	arg1	CONCLUSIONS					1364:1374	CONCLUSIONS	1364:1374	CONCLUSIONS This study evidenced that vegetative C. acetobutylicum cells rather than commonly assumed spore-forming cells	1364:1484	CONCLUSIONS This study evidenced that vegetative C. acetobutylicum cells rather than commonly assumed spore-forming cells were essentially the solvent-forming cells.
30479660	11	45	theme	solvent-forming	1507:1521	arg1	cells					1523:1527	the solvent-forming cells	1503:1527	the solvent-forming cells	1503:1527	CONCLUSIONS This study evidenced that vegetative C. acetobutylicum cells rather than commonly assumed spore-forming cells were essentially the solvent-forming cells.
30479660	13	46	theme	acetobutylicum	1711:1724	arg1	biofilm					1726:1732	C. acetobutylicum biofilm	1708:1732	C. acetobutylicum biofilm which should be valuable for understanding and development of the biofilm-based processes	1708:1822	This study provides the first physiological and molecular insights into C. acetobutylicum biofilm which should be valuable for understanding and development of the biofilm-based processes.
30479660	1	47	theme	polymeric	216:224	arg1	EPS					238:240	EPS	238:240	EPS	238:240	BACKGROUND Biofilms are cell communities wherein cells are embedded in a self-produced extracellular polymeric substances (EPS).
30479660	1	47	theme	polymeric	216:224	arg1	substances					226:235	a self-produced extracellular polymeric substances	186:235	a self-produced extracellular polymeric substances (EPS)	186:241	BACKGROUND Biofilms are cell communities wherein cells are embedded in a self-produced extracellular polymeric substances (EPS).
30479660	4	48	theme	C.	643:644	arg1	biofilm					661:667	C. acetobutylicum biofilm	643:667	C. acetobutylicum biofilm	643:667	Thus, this study is focused on revealing the cell physiology and EPS composition of C. acetobutylicum biofilm.
30479660	5	49	theme	vegetative	778:787	arg1	growth					789:794	vegetative growth	778:794	vegetative growth	778:794	RESULTS Here, we revealed a novel lifestyle of C. acetobutylicum in biofilm: elimination of sporulation and vegetative growth.
30479660	7	50	theme	first	913:917	arg1	time					919:922	the first time	909:922	the first time	909:922	Furthermore, for the first time, the biofilm polysaccharides and proteins were isolated and characterized.
30479660	0	51	from	heteropolysaccharides	67:87	arg1	rich					59:62	rich	59:62	rich	59:62	Clostridium acetobutylicum grows vegetatively in a biofilm rich in heteropolysaccharides and cytoplasmic proteins.
30479660	5	52	dep	RESULTS	670:676	arg1	revealed					687:694	revealed	687:694	revealed a novel lifestyle of C. acetobutylicum in biofilm	687:744	RESULTS Here, we revealed a novel lifestyle of C. acetobutylicum in biofilm: elimination of sporulation and vegetative growth.
30479660	10	53	theme	many	1175:1178	arg1	proteins					1205:1212	many non-classically secreted proteins	1175:1212	many non-classically secreted proteins moonlighting as adhesins	1175:1237	Also, a great variety of proteins including many non-classically secreted proteins moonlighting as adhesins were found considerably present in the biofilm, with GroEL, a S-layer protein and rubrerythrin being the most abundant ones.
30479660	0	54	theme	rich	59:62	arg1	biofilm					51:57	a biofilm	49:57	a biofilm rich in heteropolysaccharides and cytoplasmic proteins	49:112	Clostridium acetobutylicum grows vegetatively in a biofilm rich in heteropolysaccharides and cytoplasmic proteins.
30479660	10	55	theme	secreted	1196:1203	arg1	proteins					1205:1212	many non-classically secreted proteins	1175:1212	many non-classically secreted proteins moonlighting as adhesins	1175:1237	Also, a great variety of proteins including many non-classically secreted proteins moonlighting as adhesins were found considerably present in the biofilm, with GroEL, a S-layer protein and rubrerythrin being the most abundant ones.
30479660	10	56	theme	non-classically	1180:1194	arg1	proteins					1205:1212	many non-classically secreted proteins	1175:1212	many non-classically secreted proteins moonlighting as adhesins	1175:1237	Also, a great variety of proteins including many non-classically secreted proteins moonlighting as adhesins were found considerably present in the biofilm, with GroEL, a S-layer protein and rubrerythrin being the most abundant ones.
30479660	12	57	theme	secreted	1559:1566	arg1	proteins					1581:1588	The abundant non-classically secreted moonlighting proteins	1530:1588	The abundant non-classically secreted moonlighting proteins	1530:1588	The abundant non-classically secreted moonlighting proteins might be important for the biofilm formation.
30479660	12	57	theme	secreted	1559:1566	arg1	important					1599:1607	important	1599:1607	important	1599:1607	The abundant non-classically secreted moonlighting proteins might be important for the biofilm formation.
30479660	12	58	theme	non-classically	1543:1557	arg1	proteins					1581:1588	The abundant non-classically secreted moonlighting proteins	1530:1588	The abundant non-classically secreted moonlighting proteins	1530:1588	The abundant non-classically secreted moonlighting proteins might be important for the biofilm formation.
30479660	12	58	theme	non-classically	1543:1557	arg1	important					1599:1607	important	1599:1607	important	1599:1607	The abundant non-classically secreted moonlighting proteins might be important for the biofilm formation.
30479660	1	59	from	substances	226:235	arg1	embedded					174:181	embedded	174:181	embedded	174:181	BACKGROUND Biofilms are cell communities wherein cells are embedded in a self-produced extracellular polymeric substances (EPS).
30479660	1	60	theme	cell	139:142	arg1	communities					144:154	cell communities	139:154	cell communities	139:154	BACKGROUND Biofilms are cell communities wherein cells are embedded in a self-produced extracellular polymeric substances (EPS).
30479660	1	60	theme	cell	139:142	arg1	Biofilms					126:133	BACKGROUND Biofilms	115:133	BACKGROUND Biofilms	115:133	BACKGROUND Biofilms are cell communities wherein cells are embedded in a self-produced extracellular polymeric substances (EPS).
30479660	7	61	theme	biofilm	929:935	arg1	polysaccharides					937:951	the biofilm polysaccharides	925:951	the biofilm polysaccharides	925:951	Furthermore, for the first time, the biofilm polysaccharides and proteins were isolated and characterized.
30479660	4	62	dep	physiology	609:618	arg1	the					600:602	the	600:602	the	600:602	Thus, this study is focused on revealing the cell physiology and EPS composition of C. acetobutylicum biofilm.
30479660	9	63	theme	major	1054:1058	arg1	fraction					1060:1067	The major fraction	1050:1067	The major fraction	1050:1067	The major fraction consisted of predominantly glucose, mannose and aminoglucose.
30479660	4	64	theme	cell	604:607	arg1	physiology					609:618	cell physiology	604:618	cell physiology	604:618	Thus, this study is focused on revealing the cell physiology and EPS composition of C. acetobutylicum biofilm.
30479660	10	65	from	present	1263:1269	arg1	biofilm					1278:1284	the biofilm	1274:1284	the biofilm	1274:1284	Also, a great variety of proteins including many non-classically secreted proteins moonlighting as adhesins were found considerably present in the biofilm, with GroEL, a S-layer protein and rubrerythrin being the most abundant ones.
30479660	10	66	from	biofilm	1278:1284	arg1	present					1263:1269	present	1263:1269	present	1263:1269	Also, a great variety of proteins including many non-classically secreted proteins moonlighting as adhesins were found considerably present in the biofilm, with GroEL, a S-layer protein and rubrerythrin being the most abundant ones.
30479660	1	67	theme	wherein	156:162	arg1	cells					164:168	wherein cells	156:168	wherein cells	156:168	BACKGROUND Biofilms are cell communities wherein cells are embedded in a self-produced extracellular polymeric substances (EPS).
30479660	0	68	from	proteins	105:112	arg1	rich					59:62	rich	59:62	rich	59:62	Clostridium acetobutylicum grows vegetatively in a biofilm rich in heteropolysaccharides and cytoplasmic proteins.
30479660	2	69	theme	acetobutylicum	271:284	arg1	biofilm					248:254	The biofilm	244:254	The biofilm of Clostridium acetobutylicum	244:284	The biofilm of Clostridium acetobutylicum confers the cells superior phenotypes and has been extensively exploited to produce a variety of liquid biofuels and bulk chemicals.
30479660	4	70	theme	acetobutylicum	646:659	arg1	biofilm					661:667	C. acetobutylicum biofilm	643:667	C. acetobutylicum biofilm	643:667	Thus, this study is focused on revealing the cell physiology and EPS composition of C. acetobutylicum biofilm.
30479660	8	71	contain	contained	1011:1019	arg1	biofilm					1003:1009	The biofilm	999:1009	The biofilm	999:1009	The biofilm contained three heteropolysaccharides.
30479660	8	71	contain	contained	1011:1019	arg2	heteropolysaccharides					1027:1047	three heteropolysaccharides	1021:1047	three heteropolysaccharides	1021:1047	The biofilm contained three heteropolysaccharides.
30479660	5	72	theme	growth	789:794	arg1	elimination					747:757	elimination	747:757	elimination of sporulation and vegetative growth	747:794	RESULTS Here, we revealed a novel lifestyle of C. acetobutylicum in biofilm: elimination of sporulation and vegetative growth.
30479660	2	73	theme	biofuels	390:397	arg1	variety					372:378	a variety	370:378	a variety of liquid biofuels and bulk chemicals	370:416	The biofilm of Clostridium acetobutylicum confers the cells superior phenotypes and has been extensively exploited to produce a variety of liquid biofuels and bulk chemicals.
30479660	2	73	theme	biofuels	390:397	arg1	biofuels					390:397	liquid biofuels	383:397	liquid biofuels	383:397	The biofilm of Clostridium acetobutylicum confers the cells superior phenotypes and has been extensively exploited to produce a variety of liquid biofuels and bulk chemicals.
30479660	2	73	theme	biofuels	390:397	arg1	chemicals					408:416	bulk chemicals	403:416	bulk chemicals	403:416	The biofilm of Clostridium acetobutylicum confers the cells superior phenotypes and has been extensively exploited to produce a variety of liquid biofuels and bulk chemicals.
30479660	10	74	theme	S-layer	1301:1307	arg1	protein					1309:1315	a S-layer protein	1299:1315	a S-layer protein	1299:1315	Also, a great variety of proteins including many non-classically secreted proteins moonlighting as adhesins were found considerably present in the biofilm, with GroEL, a S-layer protein and rubrerythrin being the most abundant ones.
30479660	12	75	theme	moonlighting	1568:1579	arg1	proteins					1581:1588	The abundant non-classically secreted moonlighting proteins	1530:1588	The abundant non-classically secreted moonlighting proteins	1530:1588	The abundant non-classically secreted moonlighting proteins might be important for the biofilm formation.
30479660	12	75	theme	moonlighting	1568:1579	arg1	important					1599:1607	important	1599:1607	important	1599:1607	The abundant non-classically secreted moonlighting proteins might be important for the biofilm formation.
29772522	0	0	theme	biofilm	89:95	arg1	composition					97:107	the biofilm composition	85:107	the biofilm composition	85:107	Bariatric surgery drives major rearrangements of the intestinal microbiota including the biofilm composition.
29772522	4	1	theme	acetate	889:895	arg1	player					912:917	a key player	906:917	a key player to inhibit enteropathogens	906:944	The Bacteroides enterotype guarantees the strong ability to form a biofilm which allows a more efficient digestion of polysaccharides than planktonic communities and leads to the production of acetate which is a key player to inhibit enteropathogens.
29772522	4	1	theme	acetate	889:895	arg1	production					875:884	the production	871:884	the production of acetate which is a key player to inhibit enteropathogens	871:944	The Bacteroides enterotype guarantees the strong ability to form a biofilm which allows a more efficient digestion of polysaccharides than planktonic communities and leads to the production of acetate which is a key player to inhibit enteropathogens.
29772522	6	2	theme	Bariatric	1131:1139	arg1	surgery					1141:1147	Bariatric surgery	1131:1147	Bariatric surgery	1131:1147	Bariatric surgery influences the microbial composition of gut biofilm.
29772522	7	3	theme	variation	1261:1269	arg1	impact					1246:1251	the impact	1242:1251	the impact of this variation on recovery after surgery and on weight loss	1242:1314	Further studies are needed to elucidate the impact of this variation on recovery after surgery and on weight loss.
29772522	3	4	theme	obese	516:520	arg1	patients					522:529	obese patients	516:529	obese patients	516:529	Although the bariatric surgery drives major rearrangements of the gut microbiota, obese patients maintain the Prevotella enterotype before and after surgery, as shown by normal weight patients, with an increase of Bacteroides vulgatus and Bacteroides uniformis.
29772522	5	5	theme	Hafniaalvei	1016:1026	arg1	increase					1004:1011	an increase	1001:1011	an increase of Hafniaalvei (Proteobacteria), a facultative anaerobic bacterium involved in intestinal and inflammatory disorders	1001:1128	Additionally, the laparoscopic gastric bypass induces an increase of Hafniaalvei (Proteobacteria), a facultative anaerobic bacterium involved in intestinal and inflammatory disorders.
29772522	6	6	theme	gut	1189:1191	arg1	biofilm					1193:1199	gut biofilm	1189:1199	gut biofilm	1189:1199	Bariatric surgery influences the microbial composition of gut biofilm.
29772522	7	7	theme	weight	1304:1309	arg1	loss					1311:1314	weight loss	1304:1314	weight loss	1304:1314	Further studies are needed to elucidate the impact of this variation on recovery after surgery and on weight loss.
29772522	4	8	theme	strong	738:743	arg1	ability					745:751	the strong ability	734:751	the strong ability to form a biofilm which allows a more efficient digestion of polysaccharides than planktonic communities and leads to the production of acetate which is a key player to inhibit enteropathogens	734:944	The Bacteroides enterotype guarantees the strong ability to form a biofilm which allows a more efficient digestion of polysaccharides than planktonic communities and leads to the production of acetate which is a key player to inhibit enteropathogens.
29772522	4	9	theme	Bacteroides	700:710	arg1	enterotype					712:721	The Bacteroides enterotype	696:721	The Bacteroides enterotype	696:721	The Bacteroides enterotype guarantees the strong ability to form a biofilm which allows a more efficient digestion of polysaccharides than planktonic communities and leads to the production of acetate which is a key player to inhibit enteropathogens.
29772522	3	10	theme	gut	500:502	arg1	microbiota					504:513	the gut microbiota	496:513	the gut microbiota	496:513	Although the bariatric surgery drives major rearrangements of the gut microbiota, obese patients maintain the Prevotella enterotype before and after surgery, as shown by normal weight patients, with an increase of Bacteroides vulgatus and Bacteroides uniformis.
29772522	2	11	theme	biofilm	411:417	arg1	configuration					419:431	the biofilm configuration	407:431	the biofilm configuration	407:431	We analysed the changes in the intestinal microbiota of obese patients after bariatric surgery with the aim of disclosing the rearrangement of the biofilm configuration.
29772522	3	12	theme	Prevotella	544:553	arg1	enterotype					555:564	the Prevotella enterotype	540:564	the Prevotella enterotype	540:564	Although the bariatric surgery drives major rearrangements of the gut microbiota, obese patients maintain the Prevotella enterotype before and after surgery, as shown by normal weight patients, with an increase of Bacteroides vulgatus and Bacteroides uniformis.
29772522	3	13	theme	bariatric	447:455	arg1	surgery					457:463	the bariatric surgery	443:463	the bariatric surgery	443:463	Although the bariatric surgery drives major rearrangements of the gut microbiota, obese patients maintain the Prevotella enterotype before and after surgery, as shown by normal weight patients, with an increase of Bacteroides vulgatus and Bacteroides uniformis.
29772522	7	14	from	impact	1246:1251	arg1	loss					1311:1314	weight loss	1304:1314	weight loss	1304:1314	Further studies are needed to elucidate the impact of this variation on recovery after surgery and on weight loss.
29772522	7	14	from	impact	1246:1251	arg1	recovery					1274:1281	recovery	1274:1281	recovery	1274:1281	Further studies are needed to elucidate the impact of this variation on recovery after surgery and on weight loss.
29772522	0	15	theme	Bariatric	0:8	arg1	surgery					10:16	Bariatric surgery	0:16	Bariatric surgery	0:16	Bariatric surgery drives major rearrangements of the intestinal microbiota including the biofilm composition.
29772522	0	16	theme	major	25:29	arg1	rearrangements					31:44	major rearrangements	25:44	major rearrangements of the intestinal microbiota including the biofilm composition	25:107	Bariatric surgery drives major rearrangements of the intestinal microbiota including the biofilm composition.
29772522	4	17	theme	polysaccharides	814:828	arg1	digestion					801:809	a more efficient digestion	784:809	a more efficient digestion of polysaccharides than planktonic communities	784:856	The Bacteroides enterotype guarantees the strong ability to form a biofilm which allows a more efficient digestion of polysaccharides than planktonic communities and leads to the production of acetate which is a key player to inhibit enteropathogens.
29772522	5	18	theme	facultative	1048:1058	arg1	bacterium					1070:1078	a facultative anaerobic bacterium	1046:1078	a facultative anaerobic bacterium involved in intestinal and inflammatory disorders	1046:1128	Additionally, the laparoscopic gastric bypass induces an increase of Hafniaalvei (Proteobacteria), a facultative anaerobic bacterium involved in intestinal and inflammatory disorders.
29772522	5	18	theme	facultative	1048:1058	arg1	Hafniaalvei					1016:1026	Hafniaalvei	1016:1026	Hafniaalvei (Proteobacteria)	1016:1043	Additionally, the laparoscopic gastric bypass induces an increase of Hafniaalvei (Proteobacteria), a facultative anaerobic bacterium involved in intestinal and inflammatory disorders.
29772522	2	19	theme	intestinal	295:304	arg1	microbiota					306:315	the intestinal microbiota	291:315	the intestinal microbiota of obese patients	291:333	We analysed the changes in the intestinal microbiota of obese patients after bariatric surgery with the aim of disclosing the rearrangement of the biofilm configuration.
29772522	2	20	theme	configuration	419:431	arg1	rearrangement					390:402	the rearrangement	386:402	the rearrangement of the biofilm configuration	386:431	We analysed the changes in the intestinal microbiota of obese patients after bariatric surgery with the aim of disclosing the rearrangement of the biofilm configuration.
29772522	5	21	theme	intestinal	1092:1101	arg1	disorders					1120:1128	intestinal and inflammatory disorders	1092:1128	disorders	1120:1128	Additionally, the laparoscopic gastric bypass induces an increase of Hafniaalvei (Proteobacteria), a facultative anaerobic bacterium involved in intestinal and inflammatory disorders.
29772522	1	22	theme	intestinal	114:123	arg1	disequilibrium					136:149	The intestinal microbiota disequilibrium	110:149	The intestinal microbiota disequilibrium	110:149	The intestinal microbiota disequilibrium has been associated with obesity, while the role of the gut mucosal biofilms in this pathology is still unknown.
29772522	1	23	theme	gut	207:209	arg1	biofilms					219:226	the gut mucosal biofilms	203:226	the gut mucosal biofilms	203:226	The intestinal microbiota disequilibrium has been associated with obesity, while the role of the gut mucosal biofilms in this pathology is still unknown.
29772522	4	24	theme	planktonic	835:844	arg1	communities					846:856	planktonic communities	835:856	planktonic communities	835:856	The Bacteroides enterotype guarantees the strong ability to form a biofilm which allows a more efficient digestion of polysaccharides than planktonic communities and leads to the production of acetate which is a key player to inhibit enteropathogens.
29772522	1	25	theme	mucosal	211:217	arg1	biofilms					219:226	the gut mucosal biofilms	203:226	the gut mucosal biofilms	203:226	The intestinal microbiota disequilibrium has been associated with obesity, while the role of the gut mucosal biofilms in this pathology is still unknown.
29772522	7	26	theme	Further	1202:1208	arg1	studies					1210:1216	Further studies	1202:1216	Further studies	1202:1216	Further studies are needed to elucidate the impact of this variation on recovery after surgery and on weight loss.
29772522	5	27	theme	inflammatory	1107:1118	arg1	disorders					1120:1128	intestinal and inflammatory disorders	1092:1128	disorders	1120:1128	Additionally, the laparoscopic gastric bypass induces an increase of Hafniaalvei (Proteobacteria), a facultative anaerobic bacterium involved in intestinal and inflammatory disorders.
29772522	3	28	theme	normal	604:609	arg1	patients					618:625	normal weight patients	604:625	normal weight patients	604:625	Although the bariatric surgery drives major rearrangements of the gut microbiota, obese patients maintain the Prevotella enterotype before and after surgery, as shown by normal weight patients, with an increase of Bacteroides vulgatus and Bacteroides uniformis.
29772522	1	29	theme	biofilms	219:226	arg1	role					195:198	the role	191:198	the role of the gut mucosal biofilms in this pathology	191:244	The intestinal microbiota disequilibrium has been associated with obesity, while the role of the gut mucosal biofilms in this pathology is still unknown.
29772522	1	29	theme	biofilms	219:226	arg1	unknown					255:261	unknown	255:261	unknown	255:261	The intestinal microbiota disequilibrium has been associated with obesity, while the role of the gut mucosal biofilms in this pathology is still unknown.
29772522	0	30	theme	intestinal	53:62	arg1	microbiota					64:73	the intestinal microbiota	49:73	the intestinal microbiota including the biofilm composition	49:107	Bariatric surgery drives major rearrangements of the intestinal microbiota including the biofilm composition.
29772522	4	31	theme	key	908:910	arg1	player					912:917	a key player	906:917	a key player to inhibit enteropathogens	906:944	The Bacteroides enterotype guarantees the strong ability to form a biofilm which allows a more efficient digestion of polysaccharides than planktonic communities and leads to the production of acetate which is a key player to inhibit enteropathogens.
29772522	4	31	theme	key	908:910	arg1	production					875:884	the production	871:884	the production of acetate which is a key player to inhibit enteropathogens	871:944	The Bacteroides enterotype guarantees the strong ability to form a biofilm which allows a more efficient digestion of polysaccharides than planktonic communities and leads to the production of acetate which is a key player to inhibit enteropathogens.
29772522	2	32	theme	bariatric	341:349	arg1	surgery					351:357	bariatric surgery	341:357	bariatric surgery with the aim of disclosing the rearrangement of the biofilm configuration	341:431	We analysed the changes in the intestinal microbiota of obese patients after bariatric surgery with the aim of disclosing the rearrangement of the biofilm configuration.
29772522	1	33	theme	microbiota	125:134	arg1	disequilibrium					136:149	The intestinal microbiota disequilibrium	110:149	The intestinal microbiota disequilibrium	110:149	The intestinal microbiota disequilibrium has been associated with obesity, while the role of the gut mucosal biofilms in this pathology is still unknown.
29772522	5	34	theme	anaerobic	1060:1068	arg1	bacterium					1070:1078	a facultative anaerobic bacterium	1046:1078	a facultative anaerobic bacterium involved in intestinal and inflammatory disorders	1046:1128	Additionally, the laparoscopic gastric bypass induces an increase of Hafniaalvei (Proteobacteria), a facultative anaerobic bacterium involved in intestinal and inflammatory disorders.
29772522	5	34	theme	anaerobic	1060:1068	arg1	Hafniaalvei					1016:1026	Hafniaalvei	1016:1026	Hafniaalvei (Proteobacteria)	1016:1043	Additionally, the laparoscopic gastric bypass induces an increase of Hafniaalvei (Proteobacteria), a facultative anaerobic bacterium involved in intestinal and inflammatory disorders.
29772522	3	35	theme	uniformis	685:693	arg1	increase					636:643	an increase	633:643	an increase of Bacteroides vulgatus and Bacteroides uniformis	633:693	Although the bariatric surgery drives major rearrangements of the gut microbiota, obese patients maintain the Prevotella enterotype before and after surgery, as shown by normal weight patients, with an increase of Bacteroides vulgatus and Bacteroides uniformis.
29772522	3	36	theme	microbiota	504:513	arg1	rearrangements					478:491	major rearrangements	472:491	major rearrangements of the gut microbiota	472:513	Although the bariatric surgery drives major rearrangements of the gut microbiota, obese patients maintain the Prevotella enterotype before and after surgery, as shown by normal weight patients, with an increase of Bacteroides vulgatus and Bacteroides uniformis.
29772522	2	37	with	surgery	351:357	arg1	aim					368:370	the aim	364:370	the aim of disclosing the rearrangement of the biofilm configuration	364:431	We analysed the changes in the intestinal microbiota of obese patients after bariatric surgery with the aim of disclosing the rearrangement of the biofilm configuration.
29772522	2	38	theme	patients	326:333	arg1	microbiota					306:315	the intestinal microbiota	291:315	the intestinal microbiota of obese patients	291:333	We analysed the changes in the intestinal microbiota of obese patients after bariatric surgery with the aim of disclosing the rearrangement of the biofilm configuration.
29772522	6	39	theme	biofilm	1193:1199	arg1	composition					1174:1184	the microbial composition	1160:1184	the microbial composition of gut biofilm	1160:1199	Bariatric surgery influences the microbial composition of gut biofilm.
29772522	3	40	theme	vulgatus	660:667	arg1	increase					636:643	an increase	633:643	an increase of Bacteroides vulgatus and Bacteroides uniformis	633:693	Although the bariatric surgery drives major rearrangements of the gut microbiota, obese patients maintain the Prevotella enterotype before and after surgery, as shown by normal weight patients, with an increase of Bacteroides vulgatus and Bacteroides uniformis.
29772522	4	41	theme	efficient	791:799	arg1	digestion					801:809	a more efficient digestion	784:809	a more efficient digestion of polysaccharides than planktonic communities	784:856	The Bacteroides enterotype guarantees the strong ability to form a biofilm which allows a more efficient digestion of polysaccharides than planktonic communities and leads to the production of acetate which is a key player to inhibit enteropathogens.
29772522	1	42	from	role	195:198	arg1	pathology					236:244	this pathology	231:244	this pathology	231:244	The intestinal microbiota disequilibrium has been associated with obesity, while the role of the gut mucosal biofilms in this pathology is still unknown.
29772522	2	43	theme	obese	320:324	arg1	patients					326:333	obese patients	320:333	obese patients	320:333	We analysed the changes in the intestinal microbiota of obese patients after bariatric surgery with the aim of disclosing the rearrangement of the biofilm configuration.
29772522	6	44	theme	microbial	1164:1172	arg1	composition					1174:1184	the microbial composition	1160:1184	the microbial composition of gut biofilm	1160:1199	Bariatric surgery influences the microbial composition of gut biofilm.
29772522	3	45	theme	weight	611:616	arg1	patients					618:625	normal weight patients	604:625	normal weight patients	604:625	Although the bariatric surgery drives major rearrangements of the gut microbiota, obese patients maintain the Prevotella enterotype before and after surgery, as shown by normal weight patients, with an increase of Bacteroides vulgatus and Bacteroides uniformis.
29772522	3	46	theme	major	472:476	arg1	rearrangements					478:491	major rearrangements	472:491	major rearrangements of the gut microbiota	472:513	Although the bariatric surgery drives major rearrangements of the gut microbiota, obese patients maintain the Prevotella enterotype before and after surgery, as shown by normal weight patients, with an increase of Bacteroides vulgatus and Bacteroides uniformis.
29772522	2	47	from	changes	280:286	arg1	microbiota					306:315	the intestinal microbiota	291:315	the intestinal microbiota of obese patients	291:333	We analysed the changes in the intestinal microbiota of obese patients after bariatric surgery with the aim of disclosing the rearrangement of the biofilm configuration.
29772522	0	48	theme	microbiota	64:73	arg1	rearrangements					31:44	major rearrangements	25:44	major rearrangements of the intestinal microbiota including the biofilm composition	25:107	Bariatric surgery drives major rearrangements of the intestinal microbiota including the biofilm composition.
29772522	5	49	dep	gastric	978:984	arg1	bypass					986:991	bypass	986:991	bypass	986:991	Additionally, the laparoscopic gastric bypass induces an increase of Hafniaalvei (Proteobacteria), a facultative anaerobic bacterium involved in intestinal and inflammatory disorders.
29909805	2	0	theme	genes	558:562	arg1	expression					526:535	the expression	522:535	the expression of hepatic lipogenic genes in the offspring	522:579	The current study aimed to determine the sex-specific effects of maternal consumption of sucrose or HFCS-55 on the expression of hepatic lipogenic genes in the offspring.
29909805	3	1	theme	Wistar	636:641	arg1	rats					643:646	albino Wistar rats	629:646	albino Wistar rats	629:646	Liver samples were collected from offspring of albino Wistar rats provided with ad libitum access to either water (control), 10% sucrose or 10% HFCS-55 solution during pregnancy and lactation at 3 weeks (control n=16, sucrose n=22, HFCS-55 n=16) and 12 weeks (control n=16, sucrose n=10, HFCS-55 n=16) of age.
29909805	0	2	theme	rat	142:144	arg1	offspring					146:154	rat offspring	142:154	rat offspring	142:154	The effect of maternal intake of sucrose or high-fructose corn syrup (HFCS)-55 during gestation and lactation on lipogenic gene expression in rat offspring at 3 and 12 weeks of age.
29909805	1	3	theme	hepatic	379:385	arg1	metabolism					387:396	hepatic metabolism	379:396	hepatic metabolism	379:396	Perinatal exposure to sucrose or high-fructose corn syrup-55 (HFCS-55) in rats has previously been associated with altered hepatic fat content and composition post-weaning, although the effects on hepatic metabolism are unknown.
29909805	6	4	theme	other	1329:1333	arg1	genes					1353:1357	other hepatic lipogenic genes	1329:1357	other hepatic lipogenic genes	1329:1357	There were no differences in the expression of other hepatic lipogenic genes between groups at either 3 or 12 weeks.
29909805	2	5	theme	hepatic	540:546	arg1	genes					558:562	hepatic lipogenic genes	540:562	hepatic lipogenic genes	540:562	The current study aimed to determine the sex-specific effects of maternal consumption of sucrose or HFCS-55 on the expression of hepatic lipogenic genes in the offspring.
29909805	3	6	from	weeks	779:783	arg1	lactation					764:772	lactation	764:772	lactation	764:772	Liver samples were collected from offspring of albino Wistar rats provided with ad libitum access to either water (control), 10% sucrose or 10% HFCS-55 solution during pregnancy and lactation at 3 weeks (control n=16, sucrose n=22, HFCS-55 n=16) and 12 weeks (control n=16, sucrose n=10, HFCS-55 n=16) of age.
29909805	3	6	from	weeks	779:783	arg1	pregnancy					750:758	pregnancy	750:758	pregnancy	750:758	Liver samples were collected from offspring of albino Wistar rats provided with ad libitum access to either water (control), 10% sucrose or 10% HFCS-55 solution during pregnancy and lactation at 3 weeks (control n=16, sucrose n=22, HFCS-55 n=16) and 12 weeks (control n=16, sucrose n=10, HFCS-55 n=16) of age.
29909805	2	7	from	expression	526:535	arg1	offspring					571:579	the offspring	567:579	the offspring	567:579	The current study aimed to determine the sex-specific effects of maternal consumption of sucrose or HFCS-55 on the expression of hepatic lipogenic genes in the offspring.
29909805	1	8	theme	high-fructose	215:227	arg1	HFCS-55					244:250	HFCS-55	244:250	HFCS-55	244:250	Perinatal exposure to sucrose or high-fructose corn syrup-55 (HFCS-55) in rats has previously been associated with altered hepatic fat content and composition post-weaning, although the effects on hepatic metabolism are unknown.
29909805	1	8	theme	high-fructose	215:227	arg1	syrup-55					234:241	high-fructose corn syrup-55	215:241	high-fructose corn syrup-55 (HFCS-55)	215:251	Perinatal exposure to sucrose or high-fructose corn syrup-55 (HFCS-55) in rats has previously been associated with altered hepatic fat content and composition post-weaning, although the effects on hepatic metabolism are unknown.
29909805	4	9	theme	sterol	995:1000	arg1	protein-1c					1029:1038	sterol regulatory element-binding protein-1c	995:1038	sterol regulatory element-binding protein-1c	995:1038	Hepatic expression of the transcription factors such as carbohydrate response element-binding protein, sterol regulatory element-binding protein-1c and downstream genes was determined by quantitative real-time PCR.
29909805	3	10	theme	ad	662:663	arg1	access					673:678	ad libitum access	662:678	ad libitum access to either water (control), 10% sucrose or 10% HFCS-55 solution	662:741	Liver samples were collected from offspring of albino Wistar rats provided with ad libitum access to either water (control), 10% sucrose or 10% HFCS-55 solution during pregnancy and lactation at 3 weeks (control n=16, sucrose n=22, HFCS-55 n=16) and 12 weeks (control n=16, sucrose n=10, HFCS-55 n=16) of age.
29909805	7	11	theme	future	1628:1633	arg1	studies					1635:1641	future studies	1628:1641	future studies	1628:1641	Thus, perinatal exposure to sucrose may be more detrimental to offspring hepatic metabolism compared with HFCS-55, independent of sex, and it will be important to evaluate the longer-term effects of perinatal sucrose exposure in future studies.
29909805	4	12	theme	element-binding	1013:1027	arg1	protein-1c					1029:1038	sterol regulatory element-binding protein-1c	995:1038	sterol regulatory element-binding protein-1c	995:1038	Hepatic expression of the transcription factors such as carbohydrate response element-binding protein, sterol regulatory element-binding protein-1c and downstream genes was determined by quantitative real-time PCR.
29909805	3	13	theme	age	887:889	arg1	weeks					835:839	12 weeks	832:839	12 weeks (control n=16, sucrose n=10, HFCS-55 n=16)	832:882	Liver samples were collected from offspring of albino Wistar rats provided with ad libitum access to either water (control), 10% sucrose or 10% HFCS-55 solution during pregnancy and lactation at 3 weeks (control n=16, sucrose n=22, HFCS-55 n=16) and 12 weeks (control n=16, sucrose n=10, HFCS-55 n=16) of age.
29909805	3	13	theme	age	887:889	arg1	weeks					779:783	3 weeks	777:783	3 weeks (control n=16, sucrose n=22, HFCS-55 n=16)	777:826	Liver samples were collected from offspring of albino Wistar rats provided with ad libitum access to either water (control), 10% sucrose or 10% HFCS-55 solution during pregnancy and lactation at 3 weeks (control n=16, sucrose n=22, HFCS-55 n=16) and 12 weeks (control n=16, sucrose n=10, HFCS-55 n=16) of age.
29909805	4	14	theme	response	961:968	arg1	protein					986:992	carbohydrate response element-binding protein	948:992	carbohydrate response element-binding protein	948:992	Hepatic expression of the transcription factors such as carbohydrate response element-binding protein, sterol regulatory element-binding protein-1c and downstream genes was determined by quantitative real-time PCR.
29909805	3	15	theme	10	707:708	arg1	%					709:709	%	709:709	%	709:709	Liver samples were collected from offspring of albino Wistar rats provided with ad libitum access to either water (control), 10% sucrose or 10% HFCS-55 solution during pregnancy and lactation at 3 weeks (control n=16, sucrose n=22, HFCS-55 n=16) and 12 weeks (control n=16, sucrose n=10, HFCS-55 n=16) of age.
29909805	1	16	theme	post-weaning	341:352	arg1	composition					329:339	composition post-weaning	329:352	composition post-weaning	329:352	Perinatal exposure to sucrose or high-fructose corn syrup-55 (HFCS-55) in rats has previously been associated with altered hepatic fat content and composition post-weaning, although the effects on hepatic metabolism are unknown.
29909805	1	17	from	exposure	192:199	arg1	rats					256:259	rats	256:259	rats	256:259	Perinatal exposure to sucrose or high-fructose corn syrup-55 (HFCS-55) in rats has previously been associated with altered hepatic fat content and composition post-weaning, although the effects on hepatic metabolism are unknown.
29909805	0	18	theme	lipogenic	113:121	arg1	expression					128:137	lipogenic gene expression	113:137	lipogenic gene expression in rat offspring at 3 and 12 weeks of age	113:179	The effect of maternal intake of sucrose or high-fructose corn syrup (HFCS)-55 during gestation and lactation on lipogenic gene expression in rat offspring at 3 and 12 weeks of age.
29909805	7	19	theme	offspring	1462:1470	arg1	metabolism					1480:1489	offspring hepatic metabolism	1462:1489	offspring hepatic metabolism compared with HFCS-55, independent of sex	1462:1531	Thus, perinatal exposure to sucrose may be more detrimental to offspring hepatic metabolism compared with HFCS-55, independent of sex, and it will be important to evaluate the longer-term effects of perinatal sucrose exposure in future studies.
29909805	4	20	theme	quantitative	1079:1090	arg1	PCR					1102:1104	quantitative real-time PCR	1079:1104	quantitative real-time PCR	1079:1104	Hepatic expression of the transcription factors such as carbohydrate response element-binding protein, sterol regulatory element-binding protein-1c and downstream genes was determined by quantitative real-time PCR.
29909805	3	21	theme	10	722:723	arg1	%					724:724	%	724:724	%	724:724	Liver samples were collected from offspring of albino Wistar rats provided with ad libitum access to either water (control), 10% sucrose or 10% HFCS-55 solution during pregnancy and lactation at 3 weeks (control n=16, sucrose n=22, HFCS-55 n=16) and 12 weeks (control n=16, sucrose n=10, HFCS-55 n=16) of age.
29909805	7	22	theme	longer-term	1575:1585	arg1	effects					1587:1593	the longer-term effects	1571:1593	the longer-term effects of perinatal sucrose exposure in future studies	1571:1641	Thus, perinatal exposure to sucrose may be more detrimental to offspring hepatic metabolism compared with HFCS-55, independent of sex, and it will be important to evaluate the longer-term effects of perinatal sucrose exposure in future studies.
29909805	5	23	from	weeks	1237:1241	arg1	control					1200:1206	control	1200:1206	control	1200:1206	Sucrose-exposed offspring had higher hepatic SREBP-1c messenger RNA expression compared with control and HFCS-55 groups at both 3 weeks (P=0.01) and 12 weeks (P=0.03) of age.
29909805	5	23	from	weeks	1237:1241	arg1	groups					1220:1225	HFCS-55 groups	1212:1225	HFCS-55 groups at both 3 weeks (P=0.01) and 12 weeks (P=0.03) of age	1212:1279	Sucrose-exposed offspring had higher hepatic SREBP-1c messenger RNA expression compared with control and HFCS-55 groups at both 3 weeks (P=0.01) and 12 weeks (P=0.03) of age.
29909805	0	24	theme	high-fructose	44:56	arg1	HFCS					70:73	high-fructose corn syrup (HFCS)-55	44:77	high-fructose corn syrup (HFCS)-55	44:77	The effect of maternal intake of sucrose or high-fructose corn syrup (HFCS)-55 during gestation and lactation on lipogenic gene expression in rat offspring at 3 and 12 weeks of age.
29909805	3	25	theme	control	786:792	arg1	n=22					808:811	sucrose n=22	800:811	sucrose n=22	800:811	Liver samples were collected from offspring of albino Wistar rats provided with ad libitum access to either water (control), 10% sucrose or 10% HFCS-55 solution during pregnancy and lactation at 3 weeks (control n=16, sucrose n=22, HFCS-55 n=16) and 12 weeks (control n=16, sucrose n=10, HFCS-55 n=16) of age.
29909805	3	25	theme	control	786:792	arg1	n=16					822:825	HFCS-55 n=16	814:825	HFCS-55 n=16	814:825	Liver samples were collected from offspring of albino Wistar rats provided with ad libitum access to either water (control), 10% sucrose or 10% HFCS-55 solution during pregnancy and lactation at 3 weeks (control n=16, sucrose n=22, HFCS-55 n=16) and 12 weeks (control n=16, sucrose n=10, HFCS-55 n=16) of age.
29909805	3	25	theme	control	786:792	arg1	n=16					794:797	control n=16	786:797	control n=16	786:797	Liver samples were collected from offspring of albino Wistar rats provided with ad libitum access to either water (control), 10% sucrose or 10% HFCS-55 solution during pregnancy and lactation at 3 weeks (control n=16, sucrose n=22, HFCS-55 n=16) and 12 weeks (control n=16, sucrose n=10, HFCS-55 n=16) of age.
29909805	5	26	theme	RNA	1171:1173	arg1	expression					1175:1184	higher hepatic SREBP-1c messenger RNA expression	1137:1184	higher hepatic SREBP-1c messenger RNA expression	1137:1184	Sucrose-exposed offspring had higher hepatic SREBP-1c messenger RNA expression compared with control and HFCS-55 groups at both 3 weeks (P=0.01) and 12 weeks (P=0.03) of age.
29909805	0	27	theme	syrup	63:67	arg1	HFCS					70:73	high-fructose corn syrup (HFCS)-55	44:77	high-fructose corn syrup (HFCS)-55	44:77	The effect of maternal intake of sucrose or high-fructose corn syrup (HFCS)-55 during gestation and lactation on lipogenic gene expression in rat offspring at 3 and 12 weeks of age.
29909805	2	28	theme	maternal	476:483	arg1	consumption					485:495	maternal consumption	476:495	maternal consumption of sucrose or HFCS-55	476:517	The current study aimed to determine the sex-specific effects of maternal consumption of sucrose or HFCS-55 on the expression of hepatic lipogenic genes in the offspring.
29909805	3	29	theme	HFCS-55	726:732	arg1	solution					734:741	10% HFCS-55 solution	722:741	10% HFCS-55 solution	722:741	Liver samples were collected from offspring of albino Wistar rats provided with ad libitum access to either water (control), 10% sucrose or 10% HFCS-55 solution during pregnancy and lactation at 3 weeks (control n=16, sucrose n=22, HFCS-55 n=16) and 12 weeks (control n=16, sucrose n=10, HFCS-55 n=16) of age.
29909805	0	30	theme	HFCS	70:73	arg1	intake					23:28	maternal intake	14:28	maternal intake of sucrose or high-fructose corn syrup (HFCS)-55 during gestation and lactation	14:108	The effect of maternal intake of sucrose or high-fructose corn syrup (HFCS)-55 during gestation and lactation on lipogenic gene expression in rat offspring at 3 and 12 weeks of age.
29909805	1	31	theme	altered	297:303	arg1	content					317:323	altered hepatic fat content	297:323	altered hepatic fat content	297:323	Perinatal exposure to sucrose or high-fructose corn syrup-55 (HFCS-55) in rats has previously been associated with altered hepatic fat content and composition post-weaning, although the effects on hepatic metabolism are unknown.
29909805	6	32	from	weeks	1392:1396	arg1	groups					1367:1372	groups	1367:1372	groups at either 3 or 12 weeks	1367:1396	There were no differences in the expression of other hepatic lipogenic genes between groups at either 3 or 12 weeks.
29909805	1	33	theme	fat	313:315	arg1	content					317:323	altered hepatic fat content	297:323	altered hepatic fat content	297:323	Perinatal exposure to sucrose or high-fructose corn syrup-55 (HFCS-55) in rats has previously been associated with altered hepatic fat content and composition post-weaning, although the effects on hepatic metabolism are unknown.
29909805	3	34	dep	weeks	779:783	arg1	n=22					808:811	sucrose n=22	800:811	sucrose n=22	800:811	Liver samples were collected from offspring of albino Wistar rats provided with ad libitum access to either water (control), 10% sucrose or 10% HFCS-55 solution during pregnancy and lactation at 3 weeks (control n=16, sucrose n=22, HFCS-55 n=16) and 12 weeks (control n=16, sucrose n=10, HFCS-55 n=16) of age.
29909805	3	34	dep	weeks	779:783	arg1	n=16					822:825	HFCS-55 n=16	814:825	HFCS-55 n=16	814:825	Liver samples were collected from offspring of albino Wistar rats provided with ad libitum access to either water (control), 10% sucrose or 10% HFCS-55 solution during pregnancy and lactation at 3 weeks (control n=16, sucrose n=22, HFCS-55 n=16) and 12 weeks (control n=16, sucrose n=10, HFCS-55 n=16) of age.
29909805	3	34	dep	weeks	779:783	arg1	n=16					794:797	control n=16	786:797	control n=16	786:797	Liver samples were collected from offspring of albino Wistar rats provided with ad libitum access to either water (control), 10% sucrose or 10% HFCS-55 solution during pregnancy and lactation at 3 weeks (control n=16, sucrose n=22, HFCS-55 n=16) and 12 weeks (control n=16, sucrose n=10, HFCS-55 n=16) of age.
29909805	0	35	theme	corn	58:61	arg1	HFCS					70:73	high-fructose corn syrup (HFCS)-55	44:77	high-fructose corn syrup (HFCS)-55	44:77	The effect of maternal intake of sucrose or high-fructose corn syrup (HFCS)-55 during gestation and lactation on lipogenic gene expression in rat offspring at 3 and 12 weeks of age.
29909805	1	36	theme	corn	229:232	arg1	HFCS-55					244:250	HFCS-55	244:250	HFCS-55	244:250	Perinatal exposure to sucrose or high-fructose corn syrup-55 (HFCS-55) in rats has previously been associated with altered hepatic fat content and composition post-weaning, although the effects on hepatic metabolism are unknown.
29909805	1	36	theme	corn	229:232	arg1	syrup-55					234:241	high-fructose corn syrup-55	215:241	high-fructose corn syrup-55 (HFCS-55)	215:251	Perinatal exposure to sucrose or high-fructose corn syrup-55 (HFCS-55) in rats has previously been associated with altered hepatic fat content and composition post-weaning, although the effects on hepatic metabolism are unknown.
29909805	7	37	theme	sex	1529:1531	arg1	independent					1514:1524	independent	1514:1524	independent	1514:1524	Thus, perinatal exposure to sucrose may be more detrimental to offspring hepatic metabolism compared with HFCS-55, independent of sex, and it will be important to evaluate the longer-term effects of perinatal sucrose exposure in future studies.
29909805	4	38	theme	Hepatic	892:898	arg1	expression					900:909	Hepatic expression	892:909	Hepatic expression of the transcription factors such as carbohydrate response element-binding protein, sterol regulatory element-binding protein-1c and downstream genes	892:1059	Hepatic expression of the transcription factors such as carbohydrate response element-binding protein, sterol regulatory element-binding protein-1c and downstream genes was determined by quantitative real-time PCR.
29909805	7	39	theme	independent	1514:1524	arg1	HFCS-55					1505:1511	HFCS-55	1505:1511	HFCS-55	1505:1511	Thus, perinatal exposure to sucrose may be more detrimental to offspring hepatic metabolism compared with HFCS-55, independent of sex, and it will be important to evaluate the longer-term effects of perinatal sucrose exposure in future studies.
29909805	3	40	from	weeks	835:839	arg1	lactation					764:772	lactation	764:772	lactation	764:772	Liver samples were collected from offspring of albino Wistar rats provided with ad libitum access to either water (control), 10% sucrose or 10% HFCS-55 solution during pregnancy and lactation at 3 weeks (control n=16, sucrose n=22, HFCS-55 n=16) and 12 weeks (control n=16, sucrose n=10, HFCS-55 n=16) of age.
29909805	3	40	from	weeks	835:839	arg1	pregnancy					750:758	pregnancy	750:758	pregnancy	750:758	Liver samples were collected from offspring of albino Wistar rats provided with ad libitum access to either water (control), 10% sucrose or 10% HFCS-55 solution during pregnancy and lactation at 3 weeks (control n=16, sucrose n=22, HFCS-55 n=16) and 12 weeks (control n=16, sucrose n=10, HFCS-55 n=16) of age.
29909805	0	41	theme	intake	23:28	arg1	effect					4:9	The effect	0:9	The effect of maternal intake of sucrose or high-fructose corn syrup (HFCS)-55 during gestation and lactation on lipogenic gene expression in rat offspring at 3 and 12 weeks of age.	0:180	The effect of maternal intake of sucrose or high-fructose corn syrup (HFCS)-55 during gestation and lactation on lipogenic gene expression in rat offspring at 3 and 12 weeks of age.
29909805	0	42	from	weeks	168:172	arg1	expression					128:137	lipogenic gene expression	113:137	lipogenic gene expression in rat offspring at 3 and 12 weeks of age	113:179	The effect of maternal intake of sucrose or high-fructose corn syrup (HFCS)-55 during gestation and lactation on lipogenic gene expression in rat offspring at 3 and 12 weeks of age.
29909805	0	43	theme	sucrose	33:39	arg1	intake					23:28	maternal intake	14:28	maternal intake of sucrose or high-fructose corn syrup (HFCS)-55 during gestation and lactation	14:108	The effect of maternal intake of sucrose or high-fructose corn syrup (HFCS)-55 during gestation and lactation on lipogenic gene expression in rat offspring at 3 and 12 weeks of age.
29909805	3	44	theme	albino	629:634	arg1	rats					643:646	albino Wistar rats	629:646	albino Wistar rats	629:646	Liver samples were collected from offspring of albino Wistar rats provided with ad libitum access to either water (control), 10% sucrose or 10% HFCS-55 solution during pregnancy and lactation at 3 weeks (control n=16, sucrose n=22, HFCS-55 n=16) and 12 weeks (control n=16, sucrose n=10, HFCS-55 n=16) of age.
29909805	5	45	theme	age	1277:1279	arg1	weeks					1259:1263	12 weeks	1256:1263	12 weeks (P=0.03)	1256:1272	Sucrose-exposed offspring had higher hepatic SREBP-1c messenger RNA expression compared with control and HFCS-55 groups at both 3 weeks (P=0.01) and 12 weeks (P=0.03) of age.
29909805	5	45	theme	age	1277:1279	arg1	P=0.01					1244:1249	P=0.01	1244:1249	P=0.01	1244:1249	Sucrose-exposed offspring had higher hepatic SREBP-1c messenger RNA expression compared with control and HFCS-55 groups at both 3 weeks (P=0.01) and 12 weeks (P=0.03) of age.
29909805	5	45	theme	age	1277:1279	arg1	P=0.03					1266:1271	P=0.03	1266:1271	P=0.03	1266:1271	Sucrose-exposed offspring had higher hepatic SREBP-1c messenger RNA expression compared with control and HFCS-55 groups at both 3 weeks (P=0.01) and 12 weeks (P=0.03) of age.
29909805	5	45	theme	age	1277:1279	arg1	weeks					1237:1241	3 weeks	1235:1241	3 weeks (P=0.01)	1235:1250	Sucrose-exposed offspring had higher hepatic SREBP-1c messenger RNA expression compared with control and HFCS-55 groups at both 3 weeks (P=0.01) and 12 weeks (P=0.03) of age.
29909805	3	46	theme	sucrose	856:862	arg1	n=16					850:853	control n=16	842:853	control n=16	842:853	Liver samples were collected from offspring of albino Wistar rats provided with ad libitum access to either water (control), 10% sucrose or 10% HFCS-55 solution during pregnancy and lactation at 3 weeks (control n=16, sucrose n=22, HFCS-55 n=16) and 12 weeks (control n=16, sucrose n=10, HFCS-55 n=16) of age.
29909805	3	46	theme	sucrose	856:862	arg1	n=10					864:867	sucrose n=10	856:867	sucrose n=10	856:867	Liver samples were collected from offspring of albino Wistar rats provided with ad libitum access to either water (control), 10% sucrose or 10% HFCS-55 solution during pregnancy and lactation at 3 weeks (control n=16, sucrose n=22, HFCS-55 n=16) and 12 weeks (control n=16, sucrose n=10, HFCS-55 n=16) of age.
29909805	4	47	theme	carbohydrate	948:959	arg1	protein					986:992	carbohydrate response element-binding protein	948:992	carbohydrate response element-binding protein	948:992	Hepatic expression of the transcription factors such as carbohydrate response element-binding protein, sterol regulatory element-binding protein-1c and downstream genes was determined by quantitative real-time PCR.
29909805	6	48	theme	genes	1353:1357	arg1	expression					1315:1324	the expression	1311:1324	the expression of other hepatic lipogenic genes	1311:1357	There were no differences in the expression of other hepatic lipogenic genes between groups at either 3 or 12 weeks.
29909805	1	49	theme	Perinatal	182:190	arg1	exposure					192:199	Perinatal exposure	182:199	Perinatal exposure to sucrose or high-fructose corn syrup-55 (HFCS-55) in rats	182:259	Perinatal exposure to sucrose or high-fructose corn syrup-55 (HFCS-55) in rats has previously been associated with altered hepatic fat content and composition post-weaning, although the effects on hepatic metabolism are unknown.
29909805	6	50	theme	hepatic	1335:1341	arg1	genes					1353:1357	other hepatic lipogenic genes	1329:1357	other hepatic lipogenic genes	1329:1357	There were no differences in the expression of other hepatic lipogenic genes between groups at either 3 or 12 weeks.
29909805	2	51	theme	lipogenic	548:556	arg1	genes					558:562	hepatic lipogenic genes	540:562	hepatic lipogenic genes	540:562	The current study aimed to determine the sex-specific effects of maternal consumption of sucrose or HFCS-55 on the expression of hepatic lipogenic genes in the offspring.
29909805	4	52	theme	transcription	918:930	arg1	genes					1055:1059	downstream genes	1044:1059	downstream genes	1044:1059	Hepatic expression of the transcription factors such as carbohydrate response element-binding protein, sterol regulatory element-binding protein-1c and downstream genes was determined by quantitative real-time PCR.
29909805	4	52	theme	transcription	918:930	arg1	protein					986:992	carbohydrate response element-binding protein	948:992	carbohydrate response element-binding protein	948:992	Hepatic expression of the transcription factors such as carbohydrate response element-binding protein, sterol regulatory element-binding protein-1c and downstream genes was determined by quantitative real-time PCR.
29909805	4	52	theme	transcription	918:930	arg1	factors					932:938	the transcription factors	914:938	the transcription factors such as carbohydrate response element-binding protein, sterol regulatory element-binding protein-1c and downstream genes	914:1059	Hepatic expression of the transcription factors such as carbohydrate response element-binding protein, sterol regulatory element-binding protein-1c and downstream genes was determined by quantitative real-time PCR.
29909805	4	52	theme	transcription	918:930	arg1	protein-1c					1029:1038	sterol regulatory element-binding protein-1c	995:1038	sterol regulatory element-binding protein-1c	995:1038	Hepatic expression of the transcription factors such as carbohydrate response element-binding protein, sterol regulatory element-binding protein-1c and downstream genes was determined by quantitative real-time PCR.
29909805	0	53	from	expression	128:137	arg1	offspring					146:154	rat offspring	142:154	rat offspring	142:154	The effect of maternal intake of sucrose or high-fructose corn syrup (HFCS)-55 during gestation and lactation on lipogenic gene expression in rat offspring at 3 and 12 weeks of age.
29909805	3	54	theme	rats	643:646	arg1	offspring					616:624	offspring	616:624	offspring of albino Wistar rats provided with ad libitum access to either water (control), 10% sucrose or 10% HFCS-55 solution during pregnancy and lactation at 3 weeks (control n=16, sucrose n=22, HFCS-55 n=16) and 12 weeks (control n=16, sucrose n=10, HFCS-55 n=16) of age	616:889	Liver samples were collected from offspring of albino Wistar rats provided with ad libitum access to either water (control), 10% sucrose or 10% HFCS-55 solution during pregnancy and lactation at 3 weeks (control n=16, sucrose n=22, HFCS-55 n=16) and 12 weeks (control n=16, sucrose n=10, HFCS-55 n=16) of age.
29909805	5	55	theme	Sucrose-exposed	1107:1121	arg1	offspring					1123:1131	Sucrose-exposed offspring	1107:1131	Sucrose-exposed offspring	1107:1131	Sucrose-exposed offspring had higher hepatic SREBP-1c messenger RNA expression compared with control and HFCS-55 groups at both 3 weeks (P=0.01) and 12 weeks (P=0.03) of age.
29909805	5	56	contain	had	1133:1135	arg1	offspring					1123:1131	Sucrose-exposed offspring	1107:1131	Sucrose-exposed offspring	1107:1131	Sucrose-exposed offspring had higher hepatic SREBP-1c messenger RNA expression compared with control and HFCS-55 groups at both 3 weeks (P=0.01) and 12 weeks (P=0.03) of age.
29909805	5	56	contain	had	1133:1135	arg2	expression					1175:1184	higher hepatic SREBP-1c messenger RNA expression	1137:1184	higher hepatic SREBP-1c messenger RNA expression	1137:1184	Sucrose-exposed offspring had higher hepatic SREBP-1c messenger RNA expression compared with control and HFCS-55 groups at both 3 weeks (P=0.01) and 12 weeks (P=0.03) of age.
29909805	4	57	theme	regulatory	1002:1011	arg1	protein-1c					1029:1038	sterol regulatory element-binding protein-1c	995:1038	sterol regulatory element-binding protein-1c	995:1038	Hepatic expression of the transcription factors such as carbohydrate response element-binding protein, sterol regulatory element-binding protein-1c and downstream genes was determined by quantitative real-time PCR.
29909805	3	58	dep	weeks	835:839	arg1	n=16					878:881	HFCS-55 n=16	870:881	HFCS-55 n=16	870:881	Liver samples were collected from offspring of albino Wistar rats provided with ad libitum access to either water (control), 10% sucrose or 10% HFCS-55 solution during pregnancy and lactation at 3 weeks (control n=16, sucrose n=22, HFCS-55 n=16) and 12 weeks (control n=16, sucrose n=10, HFCS-55 n=16) of age.
29909805	3	58	dep	weeks	835:839	arg1	n=16					850:853	control n=16	842:853	control n=16	842:853	Liver samples were collected from offspring of albino Wistar rats provided with ad libitum access to either water (control), 10% sucrose or 10% HFCS-55 solution during pregnancy and lactation at 3 weeks (control n=16, sucrose n=22, HFCS-55 n=16) and 12 weeks (control n=16, sucrose n=10, HFCS-55 n=16) of age.
29909805	3	58	dep	weeks	835:839	arg1	n=10					864:867	sucrose n=10	856:867	sucrose n=10	856:867	Liver samples were collected from offspring of albino Wistar rats provided with ad libitum access to either water (control), 10% sucrose or 10% HFCS-55 solution during pregnancy and lactation at 3 weeks (control n=16, sucrose n=22, HFCS-55 n=16) and 12 weeks (control n=16, sucrose n=10, HFCS-55 n=16) of age.
29909805	3	59	theme	libitum	665:671	arg1	access					673:678	ad libitum access	662:678	ad libitum access to either water (control), 10% sucrose or 10% HFCS-55 solution	662:741	Liver samples were collected from offspring of albino Wistar rats provided with ad libitum access to either water (control), 10% sucrose or 10% HFCS-55 solution during pregnancy and lactation at 3 weeks (control n=16, sucrose n=22, HFCS-55 n=16) and 12 weeks (control n=16, sucrose n=10, HFCS-55 n=16) of age.
29909805	7	60	theme	sucrose	1608:1614	arg1	exposure					1616:1623	perinatal sucrose exposure	1598:1623	perinatal sucrose exposure	1598:1623	Thus, perinatal exposure to sucrose may be more detrimental to offspring hepatic metabolism compared with HFCS-55, independent of sex, and it will be important to evaluate the longer-term effects of perinatal sucrose exposure in future studies.
29909805	7	61	from	effects	1587:1593	arg1	studies					1635:1641	future studies	1628:1641	future studies	1628:1641	Thus, perinatal exposure to sucrose may be more detrimental to offspring hepatic metabolism compared with HFCS-55, independent of sex, and it will be important to evaluate the longer-term effects of perinatal sucrose exposure in future studies.
29909805	4	62	theme	element-binding	970:984	arg1	protein					986:992	carbohydrate response element-binding protein	948:992	carbohydrate response element-binding protein	948:992	Hepatic expression of the transcription factors such as carbohydrate response element-binding protein, sterol regulatory element-binding protein-1c and downstream genes was determined by quantitative real-time PCR.
29909805	2	63	theme	sucrose	500:506	arg1	consumption					485:495	maternal consumption	476:495	maternal consumption of sucrose or HFCS-55	476:517	The current study aimed to determine the sex-specific effects of maternal consumption of sucrose or HFCS-55 on the expression of hepatic lipogenic genes in the offspring.
29909805	6	64	from	differences	1296:1306	arg1	expression					1315:1324	the expression	1311:1324	the expression of other hepatic lipogenic genes	1311:1357	There were no differences in the expression of other hepatic lipogenic genes between groups at either 3 or 12 weeks.
29909805	7	65	theme	hepatic	1472:1478	arg1	metabolism					1480:1489	offspring hepatic metabolism	1462:1489	offspring hepatic metabolism compared with HFCS-55, independent of sex	1462:1531	Thus, perinatal exposure to sucrose may be more detrimental to offspring hepatic metabolism compared with HFCS-55, independent of sex, and it will be important to evaluate the longer-term effects of perinatal sucrose exposure in future studies.
29909805	2	66	theme	consumption	485:495	arg1	effects					465:471	the sex-specific effects	448:471	the sex-specific effects of maternal consumption of sucrose or HFCS-55 on the expression of hepatic lipogenic genes in the offspring	448:579	The current study aimed to determine the sex-specific effects of maternal consumption of sucrose or HFCS-55 on the expression of hepatic lipogenic genes in the offspring.
29909805	3	67	theme	%	709:709	arg1	sucrose					711:717	10% sucrose	707:717	10% sucrose	707:717	Liver samples were collected from offspring of albino Wistar rats provided with ad libitum access to either water (control), 10% sucrose or 10% HFCS-55 solution during pregnancy and lactation at 3 weeks (control n=16, sucrose n=22, HFCS-55 n=16) and 12 weeks (control n=16, sucrose n=10, HFCS-55 n=16) of age.
29909805	0	68	theme	gene	123:126	arg1	expression					128:137	lipogenic gene expression	113:137	lipogenic gene expression in rat offspring at 3 and 12 weeks of age	113:179	The effect of maternal intake of sucrose or high-fructose corn syrup (HFCS)-55 during gestation and lactation on lipogenic gene expression in rat offspring at 3 and 12 weeks of age.
29909805	2	69	from	effects	465:471	arg1	expression					526:535	the expression	522:535	the expression of hepatic lipogenic genes in the offspring	522:579	The current study aimed to determine the sex-specific effects of maternal consumption of sucrose or HFCS-55 on the expression of hepatic lipogenic genes in the offspring.
29909805	4	70	theme	real-time	1092:1100	arg1	PCR					1102:1104	quantitative real-time PCR	1079:1104	quantitative real-time PCR	1079:1104	Hepatic expression of the transcription factors such as carbohydrate response element-binding protein, sterol regulatory element-binding protein-1c and downstream genes was determined by quantitative real-time PCR.
29909805	7	71	theme	exposure	1616:1623	arg1	effects					1587:1593	the longer-term effects	1571:1593	the longer-term effects of perinatal sucrose exposure in future studies	1571:1641	Thus, perinatal exposure to sucrose may be more detrimental to offspring hepatic metabolism compared with HFCS-55, independent of sex, and it will be important to evaluate the longer-term effects of perinatal sucrose exposure in future studies.
29909805	7	72	theme	perinatal	1598:1606	arg1	exposure					1616:1623	perinatal sucrose exposure	1598:1623	perinatal sucrose exposure	1598:1623	Thus, perinatal exposure to sucrose may be more detrimental to offspring hepatic metabolism compared with HFCS-55, independent of sex, and it will be important to evaluate the longer-term effects of perinatal sucrose exposure in future studies.
29909805	2	73	theme	HFCS-55	511:517	arg1	consumption					485:495	maternal consumption	476:495	maternal consumption of sucrose or HFCS-55	476:517	The current study aimed to determine the sex-specific effects of maternal consumption of sucrose or HFCS-55 on the expression of hepatic lipogenic genes in the offspring.
29909805	4	74	theme	downstream	1044:1053	arg1	genes					1055:1059	downstream genes	1044:1059	downstream genes	1044:1059	Hepatic expression of the transcription factors such as carbohydrate response element-binding protein, sterol regulatory element-binding protein-1c and downstream genes was determined by quantitative real-time PCR.
29909805	3	75	theme	%	724:724	arg1	solution					734:741	10% HFCS-55 solution	722:741	10% HFCS-55 solution	722:741	Liver samples were collected from offspring of albino Wistar rats provided with ad libitum access to either water (control), 10% sucrose or 10% HFCS-55 solution during pregnancy and lactation at 3 weeks (control n=16, sucrose n=22, HFCS-55 n=16) and 12 weeks (control n=16, sucrose n=10, HFCS-55 n=16) of age.
29909805	1	76	from	effects	368:374	arg1	metabolism					387:396	hepatic metabolism	379:396	hepatic metabolism	379:396	Perinatal exposure to sucrose or high-fructose corn syrup-55 (HFCS-55) in rats has previously been associated with altered hepatic fat content and composition post-weaning, although the effects on hepatic metabolism are unknown.
29909805	5	77	theme	messenger	1161:1169	arg1	RNA					1171:1173	higher hepatic SREBP-1c messenger RNA	1137:1173	higher hepatic SREBP-1c messenger RNA expression	1137:1184	Sucrose-exposed offspring had higher hepatic SREBP-1c messenger RNA expression compared with control and HFCS-55 groups at both 3 weeks (P=0.01) and 12 weeks (P=0.03) of age.
29909805	0	78	from	effect	4:9	arg1	expression					128:137	lipogenic gene expression	113:137	lipogenic gene expression in rat offspring at 3 and 12 weeks of age	113:179	The effect of maternal intake of sucrose or high-fructose corn syrup (HFCS)-55 during gestation and lactation on lipogenic gene expression in rat offspring at 3 and 12 weeks of age.
29909805	0	79	theme	age	177:179	arg1	weeks					168:172	3 and 12 weeks	159:172	3 and 12 weeks of age	159:179	The effect of maternal intake of sucrose or high-fructose corn syrup (HFCS)-55 during gestation and lactation on lipogenic gene expression in rat offspring at 3 and 12 weeks of age.
29909805	7	80	theme	perinatal	1405:1413	arg1	exposure					1415:1422	perinatal exposure	1405:1422	perinatal exposure to sucrose	1405:1433	Thus, perinatal exposure to sucrose may be more detrimental to offspring hepatic metabolism compared with HFCS-55, independent of sex, and it will be important to evaluate the longer-term effects of perinatal sucrose exposure in future studies.
29909805	1	81	theme	hepatic	305:311	arg1	content					317:323	altered hepatic fat content	297:323	altered hepatic fat content	297:323	Perinatal exposure to sucrose or high-fructose corn syrup-55 (HFCS-55) in rats has previously been associated with altered hepatic fat content and composition post-weaning, although the effects on hepatic metabolism are unknown.
29909805	5	82	from	weeks	1259:1263	arg1	control					1200:1206	control	1200:1206	control	1200:1206	Sucrose-exposed offspring had higher hepatic SREBP-1c messenger RNA expression compared with control and HFCS-55 groups at both 3 weeks (P=0.01) and 12 weeks (P=0.03) of age.
29909805	5	82	from	weeks	1259:1263	arg1	groups					1220:1225	HFCS-55 groups	1212:1225	HFCS-55 groups at both 3 weeks (P=0.01) and 12 weeks (P=0.03) of age	1212:1279	Sucrose-exposed offspring had higher hepatic SREBP-1c messenger RNA expression compared with control and HFCS-55 groups at both 3 weeks (P=0.01) and 12 weeks (P=0.03) of age.
29909805	2	83	theme	sex-specific	452:463	arg1	effects					465:471	the sex-specific effects	448:471	the sex-specific effects of maternal consumption of sucrose or HFCS-55 on the expression of hepatic lipogenic genes in the offspring	448:579	The current study aimed to determine the sex-specific effects of maternal consumption of sucrose or HFCS-55 on the expression of hepatic lipogenic genes in the offspring.
29909805	6	84	from	3	1384:1384	arg1	groups					1367:1372	groups	1367:1372	groups at either 3 or 12 weeks	1367:1396	There were no differences in the expression of other hepatic lipogenic genes between groups at either 3 or 12 weeks.
29909805	3	85	theme	sucrose	800:806	arg1	n=22					808:811	sucrose n=22	800:811	sucrose n=22	800:811	Liver samples were collected from offspring of albino Wistar rats provided with ad libitum access to either water (control), 10% sucrose or 10% HFCS-55 solution during pregnancy and lactation at 3 weeks (control n=16, sucrose n=22, HFCS-55 n=16) and 12 weeks (control n=16, sucrose n=10, HFCS-55 n=16) of age.
29909805	3	85	theme	sucrose	800:806	arg1	n=16					794:797	control n=16	786:797	control n=16	786:797	Liver samples were collected from offspring of albino Wistar rats provided with ad libitum access to either water (control), 10% sucrose or 10% HFCS-55 solution during pregnancy and lactation at 3 weeks (control n=16, sucrose n=22, HFCS-55 n=16) and 12 weeks (control n=16, sucrose n=10, HFCS-55 n=16) of age.
29909805	0	86	theme	maternal	14:21	arg1	intake					23:28	maternal intake	14:28	maternal intake of sucrose or high-fructose corn syrup (HFCS)-55 during gestation and lactation	14:108	The effect of maternal intake of sucrose or high-fructose corn syrup (HFCS)-55 during gestation and lactation on lipogenic gene expression in rat offspring at 3 and 12 weeks of age.
29909805	2	87	theme	current	415:421	arg1	study					423:427	The current study	411:427	The current study	411:427	The current study aimed to determine the sex-specific effects of maternal consumption of sucrose or HFCS-55 on the expression of hepatic lipogenic genes in the offspring.
29909805	3	88	theme	Liver	582:586	arg1	samples					588:594	Liver samples	582:594	Liver samples	582:594	Liver samples were collected from offspring of albino Wistar rats provided with ad libitum access to either water (control), 10% sucrose or 10% HFCS-55 solution during pregnancy and lactation at 3 weeks (control n=16, sucrose n=22, HFCS-55 n=16) and 12 weeks (control n=16, sucrose n=10, HFCS-55 n=16) of age.
29909805	5	89	theme	hepatic	1144:1150	arg1	RNA					1171:1173	higher hepatic SREBP-1c messenger RNA	1137:1173	higher hepatic SREBP-1c messenger RNA expression	1137:1184	Sucrose-exposed offspring had higher hepatic SREBP-1c messenger RNA expression compared with control and HFCS-55 groups at both 3 weeks (P=0.01) and 12 weeks (P=0.03) of age.
29909805	3	90	theme	control	842:848	arg1	n=16					878:881	HFCS-55 n=16	870:881	HFCS-55 n=16	870:881	Liver samples were collected from offspring of albino Wistar rats provided with ad libitum access to either water (control), 10% sucrose or 10% HFCS-55 solution during pregnancy and lactation at 3 weeks (control n=16, sucrose n=22, HFCS-55 n=16) and 12 weeks (control n=16, sucrose n=10, HFCS-55 n=16) of age.
29909805	3	90	theme	control	842:848	arg1	n=16					850:853	control n=16	842:853	control n=16	842:853	Liver samples were collected from offspring of albino Wistar rats provided with ad libitum access to either water (control), 10% sucrose or 10% HFCS-55 solution during pregnancy and lactation at 3 weeks (control n=16, sucrose n=22, HFCS-55 n=16) and 12 weeks (control n=16, sucrose n=10, HFCS-55 n=16) of age.
29909805	3	90	theme	control	842:848	arg1	n=10					864:867	sucrose n=10	856:867	sucrose n=10	856:867	Liver samples were collected from offspring of albino Wistar rats provided with ad libitum access to either water (control), 10% sucrose or 10% HFCS-55 solution during pregnancy and lactation at 3 weeks (control n=16, sucrose n=22, HFCS-55 n=16) and 12 weeks (control n=16, sucrose n=10, HFCS-55 n=16) of age.
29909805	4	91	theme	factors	932:938	arg1	expression					900:909	Hepatic expression	892:909	Hepatic expression of the transcription factors such as carbohydrate response element-binding protein, sterol regulatory element-binding protein-1c and downstream genes	892:1059	Hepatic expression of the transcription factors such as carbohydrate response element-binding protein, sterol regulatory element-binding protein-1c and downstream genes was determined by quantitative real-time PCR.
29909805	5	92	theme	higher	1137:1142	arg1	RNA					1171:1173	higher hepatic SREBP-1c messenger RNA	1137:1173	higher hepatic SREBP-1c messenger RNA expression	1137:1184	Sucrose-exposed offspring had higher hepatic SREBP-1c messenger RNA expression compared with control and HFCS-55 groups at both 3 weeks (P=0.01) and 12 weeks (P=0.03) of age.
29909805	6	93	theme	lipogenic	1343:1351	arg1	genes					1353:1357	other hepatic lipogenic genes	1329:1357	other hepatic lipogenic genes	1329:1357	There were no differences in the expression of other hepatic lipogenic genes between groups at either 3 or 12 weeks.
29909805	5	94	theme	SREBP-1c	1152:1159	arg1	RNA					1171:1173	higher hepatic SREBP-1c messenger RNA	1137:1173	higher hepatic SREBP-1c messenger RNA expression	1137:1184	Sucrose-exposed offspring had higher hepatic SREBP-1c messenger RNA expression compared with control and HFCS-55 groups at both 3 weeks (P=0.01) and 12 weeks (P=0.03) of age.
29600284	1	0	theme	Shigella	251:258	arg1	species					260:266	virulent Shigella species	242:266	virulent Shigella species	242:266	The native Invaplex (InvaplexNAT) vaccine and adjuvant is an ion exchange-purified product derived from the water extract of virulent Shigella species.
29600284	16	1	theme	new	2616:2618	arg1	generation					2620:2629	a new generation	2614:2629	a new generation of Invaplex	2614:2641	The work presented here describes the production and evaluation of a new generation of Invaplex.
29600284	10	2	theme	ocular	1666:1671	arg1	challenge					1673:1681	ocular challenge	1666:1681	ocular challenge	1666:1681	Guinea pigs receiving approximately 5-fold less InvaplexAR compared to cohorts immunized with InvaplexNAT were protected from ocular challenge.
29600284	7	3	theme	serum	1161:1165	arg1	IgG					1167:1169	-IpaC serum IgG	1155:1169	-IpaC serum IgG	1155:1169	In both models, immunization induced antibody responses specific for InvaplexNAT and LPS while InvaplexAR induced markedly higher anti-IpaB and -IpaC serum IgG and IgA endpoint titers.
29600284	3	4	theme	recombinant	533:543	arg1	proteins					559:566	recombinant IpaB and IpaC proteins	533:566	recombinant IpaB and IpaC proteins	533:566	To improve product purity and immunogenicity, artificial Invaplex (InvaplexAR) was developed using recombinant IpaB and IpaC proteins and purified Shigella LPS to assemble an HMMC consisting of all three components.
29600284	4	5	theme	similar	713:719	arg1	HMMC					764:767	the previously reported HMMC	740:767	the previously reported HMMC in InvaplexNAT	740:782	Characterization of InvaplexAR by various methods demonstrated similar characteristics as the previously reported HMMC in InvaplexNAT.
29600284	4	5	theme	similar	713:719	arg1	characteristics					721:735	similar characteristics	713:735	similar characteristics	713:735	Characterization of InvaplexAR by various methods demonstrated similar characteristics as the previously reported HMMC in InvaplexNAT.
29600284	5	6	theme	LPS	882:884	arg1	quantities					852:861	greater quantities	844:861	greater quantities of IpaB, IpaC, and LPS	844:884	The well-defined InvaplexAR vaccine consistently contained greater quantities of IpaB, IpaC, and LPS than InvaplexNAT.
29600284	8	7	theme	homologous	1217:1226	arg1	protection					1228:1237	homologous protection	1217:1237	homologous protection	1217:1237	In the murine model, homologous protection was achieved with 10-fold less InvaplexAR than InvaplexNAT and mice receiving InvaplexAR lost significantly less weight than mice receiving the same amount of InvaplexNAT.
29600284	7	8	theme	higher	1134:1139	arg1	anti-IpaB					1141:1149	markedly higher anti-IpaB	1125:1149	markedly higher anti-IpaB	1125:1149	In both models, immunization induced antibody responses specific for InvaplexNAT and LPS while InvaplexAR induced markedly higher anti-IpaB and -IpaC serum IgG and IgA endpoint titers.
29600284	1	9	theme	ion	178:180	arg1	product					200:206	an ion exchange-purified product	175:206	an ion exchange-purified product derived from the water extract of virulent Shigella species	175:266	The native Invaplex (InvaplexNAT) vaccine and adjuvant is an ion exchange-purified product derived from the water extract of virulent Shigella species.
29600284	1	9	theme	ion	178:180	arg1	adjuvant					163:170	adjuvant	163:170	adjuvant	163:170	The native Invaplex (InvaplexNAT) vaccine and adjuvant is an ion exchange-purified product derived from the water extract of virulent Shigella species.
29600284	1	9	theme	ion	178:180	arg1	vaccine					151:157	The native Invaplex (InvaplexNAT) vaccine	117:157	The native Invaplex (InvaplexNAT) vaccine	117:157	The native Invaplex (InvaplexNAT) vaccine and adjuvant is an ion exchange-purified product derived from the water extract of virulent Shigella species.
29600284	18	10	dep	safety	2854:2859	arg1	the					2850:2852	the	2850:2852	the	2850:2852	The next step in the product's development will be to test the safety and immune response induced in humans immunized with Invaplex.
29600284	2	11	theme	InvaplexNAT	290:300	arg1	component					277:285	The key component	269:285	The key component of InvaplexNAT	269:300	The key component of InvaplexNAT is a high-molecular-mass complex (HMMC) consisting of the Shigella lipopolysaccharide (LPS) and the invasin proteins IpaB and IpaC.
29600284	2	11	theme	InvaplexNAT	290:300	arg1	complex					327:333	a high-molecular-mass complex	305:333	a high-molecular-mass complex (HMMC) consisting of the Shigella lipopolysaccharide (LPS) and the invasin proteins IpaB and IpaC	305:431	The key component of InvaplexNAT is a high-molecular-mass complex (HMMC) consisting of the Shigella lipopolysaccharide (LPS) and the invasin proteins IpaB and IpaC.
29600284	6	12	theme	dose	986:989	arg1	studies					1002:1008	dose escalation studies	986:1008	dose escalation studies	986:1008	InvaplexAR and InvaplexNAT immunogenicities were compared in mouse and guinea pig dose escalation studies.
29600284	6	13	dep	mouse	965:969	arg1	studies					1002:1008	dose escalation studies	986:1008	dose escalation studies	986:1008	InvaplexAR and InvaplexNAT immunogenicities were compared in mouse and guinea pig dose escalation studies.
29600284	7	14	theme	antibody	1048:1055	arg1	responses					1057:1065	antibody responses	1048:1065	antibody responses specific for InvaplexNAT and LPS	1048:1098	In both models, immunization induced antibody responses specific for InvaplexNAT and LPS while InvaplexAR induced markedly higher anti-IpaB and -IpaC serum IgG and IgA endpoint titers.
29600284	8	15	theme	less	1265:1268	arg1	InvaplexAR					1270:1279	10-fold less InvaplexAR	1257:1279	10-fold less InvaplexAR than InvaplexNAT	1257:1296	In the murine model, homologous protection was achieved with 10-fold less InvaplexAR than InvaplexNAT and mice receiving InvaplexAR lost significantly less weight than mice receiving the same amount of InvaplexNAT.
29600284	6	16	theme	guinea	975:980	arg1	pig					982:984	guinea pig	975:984	guinea pig	975:984	InvaplexAR and InvaplexNAT immunogenicities were compared in mouse and guinea pig dose escalation studies.
29600284	5	17	contain	contained	834:842	arg1	vaccine					813:819	The well-defined InvaplexAR vaccine	785:819	The well-defined InvaplexAR vaccine	785:819	The well-defined InvaplexAR vaccine consistently contained greater quantities of IpaB, IpaC, and LPS than InvaplexNAT.
29600284	5	17	contain	contained	834:842	arg2	quantities					852:861	greater quantities	844:861	greater quantities of IpaB, IpaC, and LPS	844:884	The well-defined InvaplexAR vaccine consistently contained greater quantities of IpaB, IpaC, and LPS than InvaplexNAT.
29600284	8	18	theme	less	1347:1350	arg1	weight					1352:1357	significantly less weight	1333:1357	significantly less weight	1333:1357	In the murine model, homologous protection was achieved with 10-fold less InvaplexAR than InvaplexNAT and mice receiving InvaplexAR lost significantly less weight than mice receiving the same amount of InvaplexNAT.
29600284	14	19	theme	effective	2312:2320	arg1	vaccine					2322:2328	an effective vaccine	2309:2328	an effective vaccine	2309:2328	Treatment of shigellosis includes oral fluids and antibiotics, but the high burden of disease, increasing prevalence of antibiotic resistance, and long-term health consequences clearly warrant the development of an effective vaccine.
29600284	14	20	theme	long-term	2244:2252	arg1	consequences					2261:2272	long-term health consequences	2244:2272	long-term health consequences	2244:2272	Treatment of shigellosis includes oral fluids and antibiotics, but the high burden of disease, increasing prevalence of antibiotic resistance, and long-term health consequences clearly warrant the development of an effective vaccine.
29600284	3	21	theme	purified	572:579	arg1	LPS					590:592	purified Shigella LPS	572:592	purified Shigella LPS	572:592	To improve product purity and immunogenicity, artificial Invaplex (InvaplexAR) was developed using recombinant IpaB and IpaC proteins and purified Shigella LPS to assemble an HMMC consisting of all three components.
29600284	16	22	dep	production	2585:2594	arg1	the					2581:2583	the	2581:2583	the	2581:2583	The work presented here describes the production and evaluation of a new generation of Invaplex.
29600284	1	23	theme	native	121:126	arg1	product					200:206	an ion exchange-purified product	175:206	an ion exchange-purified product derived from the water extract of virulent Shigella species	175:266	The native Invaplex (InvaplexNAT) vaccine and adjuvant is an ion exchange-purified product derived from the water extract of virulent Shigella species.
29600284	1	23	theme	native	121:126	arg1	adjuvant					163:170	adjuvant	163:170	adjuvant	163:170	The native Invaplex (InvaplexNAT) vaccine and adjuvant is an ion exchange-purified product derived from the water extract of virulent Shigella species.
29600284	1	23	theme	native	121:126	arg1	vaccine					151:157	The native Invaplex (InvaplexNAT) vaccine	117:157	The native Invaplex (InvaplexNAT) vaccine	117:157	The native Invaplex (InvaplexNAT) vaccine and adjuvant is an ion exchange-purified product derived from the water extract of virulent Shigella species.
29600284	13	24	theme	young	2081:2085	arg1	children					2087:2094	young children	2081:2094	young children	2081:2094	IMPORTANCEShigella species are bacteria that cause severe diarrheal disease worldwide, primarily in young children.
29600284	18	25	from	step	2800:2803	arg1	development					2822:2832	the product's development	2808:2832	the product's development	2808:2832	The next step in the product's development will be to test the safety and immune response induced in humans immunized with Invaplex.
29600284	15	26	theme	specific	2459:2466	arg1	antigens					2468:2475	specific antigens	2459:2475	specific antigens of the bacteria	2459:2491	One Shigella vaccine under development is termed the invasin complex or Invaplex and is designed to drive an immune response to specific antigens of the bacteria in an effort to protect an individual from infection.
29600284	15	27	theme	invasin	2384:2390	arg1	complex					2392:2398	the invasin complex	2380:2398	the invasin complex	2380:2398	One Shigella vaccine under development is termed the invasin complex or Invaplex and is designed to drive an immune response to specific antigens of the bacteria in an effort to protect an individual from infection.
29600284	18	28	theme	next	2795:2798	arg1	step					2800:2803	The next step	2791:2803	The next step in the product's development	2791:2832	The next step in the product's development will be to test the safety and immune response induced in humans immunized with Invaplex.
29600284	8	29	theme	same	1383:1386	arg1	InvaplexNAT					1398:1408	InvaplexNAT	1398:1408	InvaplexNAT	1398:1408	In the murine model, homologous protection was achieved with 10-fold less InvaplexAR than InvaplexNAT and mice receiving InvaplexAR lost significantly less weight than mice receiving the same amount of InvaplexNAT.
29600284	8	29	theme	same	1383:1386	arg1	amount					1388:1393	the same amount	1379:1393	the same amount of InvaplexNAT	1379:1408	In the murine model, homologous protection was achieved with 10-fold less InvaplexAR than InvaplexNAT and mice receiving InvaplexAR lost significantly less weight than mice receiving the same amount of InvaplexNAT.
29600284	16	30	theme	Invaplex	2634:2641	arg1	generation					2620:2629	a new generation	2614:2629	a new generation of Invaplex	2614:2641	The work presented here describes the production and evaluation of a new generation of Invaplex.
29600284	18	31	theme	immune	2865:2870	arg1	response					2872:2879	immune response	2865:2879	immune response	2865:2879	The next step in the product's development will be to test the safety and immune response induced in humans immunized with Invaplex.
29600284	0	32	theme	Shigella	87:94	arg1	Invaplex					107:114	Shigella Artificial Invaplex	87:114	Shigella Artificial Invaplex	87:114	Assembly, Biochemical Characterization, Immunogenicity, Adjuvanticity, and Efficacy of Shigella Artificial Invaplex.
29600284	14	33	theme	disease	2183:2189	arg1	consequences					2261:2272	long-term health consequences	2244:2272	long-term health consequences	2244:2272	Treatment of shigellosis includes oral fluids and antibiotics, but the high burden of disease, increasing prevalence of antibiotic resistance, and long-term health consequences clearly warrant the development of an effective vaccine.
29600284	14	33	theme	disease	2183:2189	arg1	burden					2173:2178	the high burden	2164:2178	the high burden of disease	2164:2189	Treatment of shigellosis includes oral fluids and antibiotics, but the high burden of disease, increasing prevalence of antibiotic resistance, and long-term health consequences clearly warrant the development of an effective vaccine.
29600284	14	33	theme	disease	2183:2189	arg1	prevalence					2203:2212	increasing prevalence	2192:2212	increasing prevalence of antibiotic resistance	2192:2237	Treatment of shigellosis includes oral fluids and antibiotics, but the high burden of disease, increasing prevalence of antibiotic resistance, and long-term health consequences clearly warrant the development of an effective vaccine.
29600284	17	34	theme	guinea	2727:2732	arg1	pigs					2734:2737	guinea pigs	2727:2737	guinea pigs	2727:2737	The improved vaccine stimulates the production of antibodies in immunized mice and guinea pigs and protects these animals from Shigella infection.
29600284	0	35	theme	Invaplex	107:114	arg1	Efficacy					75:82	Efficacy	75:82	Efficacy	75:82	Assembly, Biochemical Characterization, Immunogenicity, Adjuvanticity, and Efficacy of Shigella Artificial Invaplex.
29600284	0	35	theme	Invaplex	107:114	arg1	Immunogenicity					40:53	Immunogenicity	40:53	Immunogenicity	40:53	Assembly, Biochemical Characterization, Immunogenicity, Adjuvanticity, and Efficacy of Shigella Artificial Invaplex.
29600284	0	35	theme	Invaplex	107:114	arg1	Assembly					0:7	Assembly	0:7	Assembly	0:7	Assembly, Biochemical Characterization, Immunogenicity, Adjuvanticity, and Efficacy of Shigella Artificial Invaplex.
29600284	0	35	theme	Invaplex	107:114	arg1	Characterization					22:37	Biochemical Characterization	10:37	Biochemical Characterization	10:37	Assembly, Biochemical Characterization, Immunogenicity, Adjuvanticity, and Efficacy of Shigella Artificial Invaplex.
29600284	0	35	theme	Invaplex	107:114	arg1	Adjuvanticity					56:68	Adjuvanticity	56:68	Adjuvanticity	56:68	Assembly, Biochemical Characterization, Immunogenicity, Adjuvanticity, and Efficacy of Shigella Artificial Invaplex.
29600284	4	36	from	HMMC	764:767	arg1	InvaplexNAT					772:782	InvaplexNAT	772:782	InvaplexNAT	772:782	Characterization of InvaplexAR by various methods demonstrated similar characteristics as the previously reported HMMC in InvaplexNAT.
29600284	12	37	theme	Shigella	1800:1807	arg1	InvaplexAR					1827:1836	InvaplexAR	1827:1836	InvaplexAR	1827:1836	The second-generation Shigella Invaplex vaccine, InvaplexAR, offers significant advantages over InvaplexNAT in reproducibility, flexible yet defined composition, immunogenicity, and protective efficacy.
29600284	12	37	theme	Shigella	1800:1807	arg1	vaccine					1818:1824	The second-generation Shigella Invaplex vaccine	1778:1824	The second-generation Shigella Invaplex vaccine	1778:1824	The second-generation Shigella Invaplex vaccine, InvaplexAR, offers significant advantages over InvaplexNAT in reproducibility, flexible yet defined composition, immunogenicity, and protective efficacy.
29600284	13	38	theme	diarrheal	2039:2047	arg1	worldwide					2057:2065	severe diarrheal disease worldwide	2032:2065	severe diarrheal disease worldwide	2032:2065	IMPORTANCEShigella species are bacteria that cause severe diarrheal disease worldwide, primarily in young children.
29600284	5	39	theme	greater	844:850	arg1	quantities					852:861	greater quantities	844:861	greater quantities of IpaB, IpaC, and LPS	844:884	The well-defined InvaplexAR vaccine consistently contained greater quantities of IpaB, IpaC, and LPS than InvaplexNAT.
29600284	10	40	theme	less	1583:1586	arg1	InvaplexAR					1588:1597	approximately 5-fold less InvaplexAR	1562:1597	approximately 5-fold less InvaplexAR	1562:1597	Guinea pigs receiving approximately 5-fold less InvaplexAR compared to cohorts immunized with InvaplexNAT were protected from ocular challenge.
29600284	3	41	theme	product	445:451	arg1	purity					453:458	product purity	445:458	product purity	445:458	To improve product purity and immunogenicity, artificial Invaplex (InvaplexAR) was developed using recombinant IpaB and IpaC proteins and purified Shigella LPS to assemble an HMMC consisting of all three components.
29600284	0	42	theme	Biochemical	10:20	arg1	Characterization					22:37	Biochemical Characterization	10:37	Biochemical Characterization	10:37	Assembly, Biochemical Characterization, Immunogenicity, Adjuvanticity, and Efficacy of Shigella Artificial Invaplex.
29600284	5	43	theme	InvaplexAR	802:811	arg1	vaccine					813:819	The well-defined InvaplexAR vaccine	785:819	The well-defined InvaplexAR vaccine	785:819	The well-defined InvaplexAR vaccine consistently contained greater quantities of IpaB, IpaC, and LPS than InvaplexNAT.
29600284	12	44	theme	protective	1960:1969	arg1	efficacy					1971:1978	protective efficacy	1960:1978	protective efficacy	1960:1978	The second-generation Shigella Invaplex vaccine, InvaplexAR, offers significant advantages over InvaplexNAT in reproducibility, flexible yet defined composition, immunogenicity, and protective efficacy.
29600284	14	45	theme	antibiotic	2217:2226	arg1	resistance					2228:2237	antibiotic resistance	2217:2237	antibiotic resistance	2217:2237	Treatment of shigellosis includes oral fluids and antibiotics, but the high burden of disease, increasing prevalence of antibiotic resistance, and long-term health consequences clearly warrant the development of an effective vaccine.
29600284	2	46	dep	proteins	410:417	arg1	IpaC					428:431	IpaC	428:431	IpaC	428:431	The key component of InvaplexNAT is a high-molecular-mass complex (HMMC) consisting of the Shigella lipopolysaccharide (LPS) and the invasin proteins IpaB and IpaC.
29600284	2	46	dep	proteins	410:417	arg1	IpaB					419:422	IpaB	419:422	IpaB	419:422	The key component of InvaplexNAT is a high-molecular-mass complex (HMMC) consisting of the Shigella lipopolysaccharide (LPS) and the invasin proteins IpaB and IpaC.
29600284	2	46	dep	proteins	410:417	arg1	proteins					410:417	the invasin proteins IpaB and IpaC	398:431	the invasin proteins IpaB and IpaC	398:431	The key component of InvaplexNAT is a high-molecular-mass complex (HMMC) consisting of the Shigella lipopolysaccharide (LPS) and the invasin proteins IpaB and IpaC.
29600284	7	47	theme	endpoint	1179:1186	arg1	titers					1188:1193	endpoint titers	1179:1193	endpoint titers	1179:1193	In both models, immunization induced antibody responses specific for InvaplexNAT and LPS while InvaplexAR induced markedly higher anti-IpaB and -IpaC serum IgG and IgA endpoint titers.
29600284	14	48	theme	oral	2131:2134	arg1	fluids					2136:2141	oral fluids	2131:2141	oral fluids	2131:2141	Treatment of shigellosis includes oral fluids and antibiotics, but the high burden of disease, increasing prevalence of antibiotic resistance, and long-term health consequences clearly warrant the development of an effective vaccine.
29600284	17	49	theme	improved	2648:2655	arg1	vaccine					2657:2663	The improved vaccine	2644:2663	The improved vaccine	2644:2663	The improved vaccine stimulates the production of antibodies in immunized mice and guinea pigs and protects these animals from Shigella infection.
29600284	9	50	with	challenge	1472:1480	arg1	serotypes					1529:1537	both homologous and heterologous Shigella serotypes	1487:1537	both homologous and heterologous Shigella serotypes	1487:1537	Moreover, mice immunized with InvaplexAR were protected from challenge with both homologous and heterologous Shigella serotypes.
29600284	14	51	theme	shigellosis	2110:2120	arg1	Treatment					2097:2105	Treatment	2097:2105	Treatment of shigellosis	2097:2120	Treatment of shigellosis includes oral fluids and antibiotics, but the high burden of disease, increasing prevalence of antibiotic resistance, and long-term health consequences clearly warrant the development of an effective vaccine.
29600284	1	52	attach	derived	208:214	arg1	extract					231:237	the water extract	221:237	the water extract of virulent Shigella species	221:266	The native Invaplex (InvaplexNAT) vaccine and adjuvant is an ion exchange-purified product derived from the water extract of virulent Shigella species.
29600284	1	52	attach	derived	208:214	arg2	adjuvant					163:170	adjuvant	163:170	adjuvant	163:170	The native Invaplex (InvaplexNAT) vaccine and adjuvant is an ion exchange-purified product derived from the water extract of virulent Shigella species.
29600284	1	52	attach	derived	208:214	arg2	product					200:206	an ion exchange-purified product	175:206	an ion exchange-purified product derived from the water extract of virulent Shigella species	175:266	The native Invaplex (InvaplexNAT) vaccine and adjuvant is an ion exchange-purified product derived from the water extract of virulent Shigella species.
29600284	1	52	attach	derived	208:214	arg2	vaccine					151:157	The native Invaplex (InvaplexNAT) vaccine	117:157	The native Invaplex (InvaplexNAT) vaccine	117:157	The native Invaplex (InvaplexNAT) vaccine and adjuvant is an ion exchange-purified product derived from the water extract of virulent Shigella species.
29600284	2	53	theme	Shigella	360:367	arg1	LPS					389:391	LPS	389:391	LPS	389:391	The key component of InvaplexNAT is a high-molecular-mass complex (HMMC) consisting of the Shigella lipopolysaccharide (LPS) and the invasin proteins IpaB and IpaC.
29600284	2	53	theme	Shigella	360:367	arg1	lipopolysaccharide					369:386	the Shigella lipopolysaccharide	356:386	the Shigella lipopolysaccharide (LPS)	356:392	The key component of InvaplexNAT is a high-molecular-mass complex (HMMC) consisting of the Shigella lipopolysaccharide (LPS) and the invasin proteins IpaB and IpaC.
29600284	15	54	theme	bacteria	2484:2491	arg1	antigens					2468:2475	specific antigens	2459:2475	specific antigens of the bacteria	2459:2491	One Shigella vaccine under development is termed the invasin complex or Invaplex and is designed to drive an immune response to specific antigens of the bacteria in an effort to protect an individual from infection.
29600284	1	55	theme	virulent	242:249	arg1	species					260:266	virulent Shigella species	242:266	virulent Shigella species	242:266	The native Invaplex (InvaplexNAT) vaccine and adjuvant is an ion exchange-purified product derived from the water extract of virulent Shigella species.
29600284	16	56	theme	generation	2620:2629	arg1	production					2585:2594	production	2585:2594	production	2585:2594	The work presented here describes the production and evaluation of a new generation of Invaplex.
29600284	16	56	theme	generation	2620:2629	arg1	evaluation					2600:2609	evaluation	2600:2609	evaluation	2600:2609	The work presented here describes the production and evaluation of a new generation of Invaplex.
29600284	12	57	theme	flexible	1906:1913	arg1	composition					1927:1937	flexible yet defined composition	1906:1937	flexible yet defined composition	1906:1937	The second-generation Shigella Invaplex vaccine, InvaplexAR, offers significant advantages over InvaplexNAT in reproducibility, flexible yet defined composition, immunogenicity, and protective efficacy.
29600284	17	58	theme	antibodies	2694:2703	arg1	production					2680:2689	the production	2676:2689	the production of antibodies	2676:2703	The improved vaccine stimulates the production of antibodies in immunized mice and guinea pigs and protects these animals from Shigella infection.
29600284	7	59	theme	-IpaC	1155:1159	arg1	IgG					1167:1169	-IpaC serum IgG	1155:1169	-IpaC serum IgG	1155:1169	In both models, immunization induced antibody responses specific for InvaplexNAT and LPS while InvaplexAR induced markedly higher anti-IpaB and -IpaC serum IgG and IgA endpoint titers.
29600284	1	60	theme	species	260:266	arg1	extract					231:237	the water extract	221:237	the water extract of virulent Shigella species	221:266	The native Invaplex (InvaplexNAT) vaccine and adjuvant is an ion exchange-purified product derived from the water extract of virulent Shigella species.
29600284	3	61	theme	IpaC	554:557	arg1	proteins					559:566	recombinant IpaB and IpaC proteins	533:566	recombinant IpaB and IpaC proteins	533:566	To improve product purity and immunogenicity, artificial Invaplex (InvaplexAR) was developed using recombinant IpaB and IpaC proteins and purified Shigella LPS to assemble an HMMC consisting of all three components.
29600284	6	62	theme	InvaplexNAT	919:929	arg1	immunogenicities					931:946	InvaplexAR and InvaplexNAT immunogenicities	904:946	InvaplexAR and InvaplexNAT immunogenicities	904:946	InvaplexAR and InvaplexNAT immunogenicities were compared in mouse and guinea pig dose escalation studies.
29600284	12	63	theme	defined	1919:1925	arg1	composition					1927:1937	flexible yet defined composition	1906:1937	flexible yet defined composition	1906:1937	The second-generation Shigella Invaplex vaccine, InvaplexAR, offers significant advantages over InvaplexNAT in reproducibility, flexible yet defined composition, immunogenicity, and protective efficacy.
29600284	2	64	theme	key	273:275	arg1	component					277:285	The key component	269:285	The key component of InvaplexNAT	269:300	The key component of InvaplexNAT is a high-molecular-mass complex (HMMC) consisting of the Shigella lipopolysaccharide (LPS) and the invasin proteins IpaB and IpaC.
29600284	2	64	theme	key	273:275	arg1	complex					327:333	a high-molecular-mass complex	305:333	a high-molecular-mass complex (HMMC) consisting of the Shigella lipopolysaccharide (LPS) and the invasin proteins IpaB and IpaC	305:431	The key component of InvaplexNAT is a high-molecular-mass complex (HMMC) consisting of the Shigella lipopolysaccharide (LPS) and the invasin proteins IpaB and IpaC.
29600284	15	65	theme	Shigella	2335:2342	arg1	vaccine					2344:2350	One Shigella vaccine	2331:2350	One Shigella vaccine under development	2331:2368	One Shigella vaccine under development is termed the invasin complex or Invaplex and is designed to drive an immune response to specific antigens of the bacteria in an effort to protect an individual from infection.
29600284	9	66	theme	homologous	1492:1501	arg1	serotypes					1529:1537	both homologous and heterologous Shigella serotypes	1487:1537	both homologous and heterologous Shigella serotypes	1487:1537	Moreover, mice immunized with InvaplexAR were protected from challenge with both homologous and heterologous Shigella serotypes.
29600284	8	67	theme	murine	1203:1208	arg1	model					1210:1214	the murine model	1199:1214	the murine model	1199:1214	In the murine model, homologous protection was achieved with 10-fold less InvaplexAR than InvaplexNAT and mice receiving InvaplexAR lost significantly less weight than mice receiving the same amount of InvaplexNAT.
29600284	6	68	theme	InvaplexAR	904:913	arg1	immunogenicities					931:946	InvaplexAR and InvaplexNAT immunogenicities	904:946	InvaplexAR and InvaplexNAT immunogenicities	904:946	InvaplexAR and InvaplexNAT immunogenicities were compared in mouse and guinea pig dose escalation studies.
29600284	2	69	theme	high-molecular-mass	307:325	arg1	component					277:285	The key component	269:285	The key component of InvaplexNAT	269:300	The key component of InvaplexNAT is a high-molecular-mass complex (HMMC) consisting of the Shigella lipopolysaccharide (LPS) and the invasin proteins IpaB and IpaC.
29600284	2	69	theme	high-molecular-mass	307:325	arg1	HMMC					336:339	HMMC	336:339	HMMC	336:339	The key component of InvaplexNAT is a high-molecular-mass complex (HMMC) consisting of the Shigella lipopolysaccharide (LPS) and the invasin proteins IpaB and IpaC.
29600284	2	69	theme	high-molecular-mass	307:325	arg1	complex					327:333	a high-molecular-mass complex	305:333	a high-molecular-mass complex (HMMC) consisting of the Shigella lipopolysaccharide (LPS) and the invasin proteins IpaB and IpaC	305:431	The key component of InvaplexNAT is a high-molecular-mass complex (HMMC) consisting of the Shigella lipopolysaccharide (LPS) and the invasin proteins IpaB and IpaC.
29600284	9	70	theme	heterologous	1507:1518	arg1	serotypes					1529:1537	both homologous and heterologous Shigella serotypes	1487:1537	both homologous and heterologous Shigella serotypes	1487:1537	Moreover, mice immunized with InvaplexAR were protected from challenge with both homologous and heterologous Shigella serotypes.
29600284	7	71	dep	IgG	1167:1169	arg1	titers					1188:1193	endpoint titers	1179:1193	endpoint titers	1179:1193	In both models, immunization induced antibody responses specific for InvaplexNAT and LPS while InvaplexAR induced markedly higher anti-IpaB and -IpaC serum IgG and IgA endpoint titers.
29600284	3	72	theme	IpaB	545:548	arg1	proteins					559:566	recombinant IpaB and IpaC proteins	533:566	recombinant IpaB and IpaC proteins	533:566	To improve product purity and immunogenicity, artificial Invaplex (InvaplexAR) was developed using recombinant IpaB and IpaC proteins and purified Shigella LPS to assemble an HMMC consisting of all three components.
29600284	5	73	theme	IpaB	866:869	arg1	quantities					852:861	greater quantities	844:861	greater quantities of IpaB, IpaC, and LPS	844:884	The well-defined InvaplexAR vaccine consistently contained greater quantities of IpaB, IpaC, and LPS than InvaplexNAT.
29600284	6	74	theme	escalation	991:1000	arg1	studies					1002:1008	dose escalation studies	986:1008	dose escalation studies	986:1008	InvaplexAR and InvaplexNAT immunogenicities were compared in mouse and guinea pig dose escalation studies.
29600284	3	75	theme	Shigella	581:588	arg1	LPS					590:592	purified Shigella LPS	572:592	purified Shigella LPS	572:592	To improve product purity and immunogenicity, artificial Invaplex (InvaplexAR) was developed using recombinant IpaB and IpaC proteins and purified Shigella LPS to assemble an HMMC consisting of all three components.
29600284	9	76	theme	Shigella	1520:1527	arg1	serotypes					1529:1537	both homologous and heterologous Shigella serotypes	1487:1537	both homologous and heterologous Shigella serotypes	1487:1537	Moreover, mice immunized with InvaplexAR were protected from challenge with both homologous and heterologous Shigella serotypes.
29600284	5	77	theme	IpaC	872:875	arg1	quantities					852:861	greater quantities	844:861	greater quantities of IpaB, IpaC, and LPS	844:884	The well-defined InvaplexAR vaccine consistently contained greater quantities of IpaB, IpaC, and LPS than InvaplexNAT.
29600284	14	78	theme	vaccine	2322:2328	arg1	development					2294:2304	the development	2290:2304	the development of an effective vaccine	2290:2328	Treatment of shigellosis includes oral fluids and antibiotics, but the high burden of disease, increasing prevalence of antibiotic resistance, and long-term health consequences clearly warrant the development of an effective vaccine.
29600284	17	79	theme	Shigella	2771:2778	arg1	infection					2780:2788	Shigella infection	2771:2788	Shigella infection	2771:2788	The improved vaccine stimulates the production of antibodies in immunized mice and guinea pigs and protects these animals from Shigella infection.
29600284	7	80	theme	specific	1067:1074	arg1	responses					1057:1065	antibody responses	1048:1065	antibody responses specific for InvaplexNAT and LPS	1048:1098	In both models, immunization induced antibody responses specific for InvaplexNAT and LPS while InvaplexAR induced markedly higher anti-IpaB and -IpaC serum IgG and IgA endpoint titers.
29600284	1	81	theme	Invaplex	128:135	arg1	product					200:206	an ion exchange-purified product	175:206	an ion exchange-purified product derived from the water extract of virulent Shigella species	175:266	The native Invaplex (InvaplexNAT) vaccine and adjuvant is an ion exchange-purified product derived from the water extract of virulent Shigella species.
29600284	1	81	theme	Invaplex	128:135	arg1	adjuvant					163:170	adjuvant	163:170	adjuvant	163:170	The native Invaplex (InvaplexNAT) vaccine and adjuvant is an ion exchange-purified product derived from the water extract of virulent Shigella species.
29600284	1	81	theme	Invaplex	128:135	arg1	vaccine					151:157	The native Invaplex (InvaplexNAT) vaccine	117:157	The native Invaplex (InvaplexNAT) vaccine	117:157	The native Invaplex (InvaplexNAT) vaccine and adjuvant is an ion exchange-purified product derived from the water extract of virulent Shigella species.
29600284	1	82	theme	exchange-purified	182:198	arg1	product					200:206	an ion exchange-purified product	175:206	an ion exchange-purified product derived from the water extract of virulent Shigella species	175:266	The native Invaplex (InvaplexNAT) vaccine and adjuvant is an ion exchange-purified product derived from the water extract of virulent Shigella species.
29600284	1	82	theme	exchange-purified	182:198	arg1	adjuvant					163:170	adjuvant	163:170	adjuvant	163:170	The native Invaplex (InvaplexNAT) vaccine and adjuvant is an ion exchange-purified product derived from the water extract of virulent Shigella species.
29600284	1	82	theme	exchange-purified	182:198	arg1	vaccine					151:157	The native Invaplex (InvaplexNAT) vaccine	117:157	The native Invaplex (InvaplexNAT) vaccine	117:157	The native Invaplex (InvaplexNAT) vaccine and adjuvant is an ion exchange-purified product derived from the water extract of virulent Shigella species.
29600284	1	83	theme	InvaplexNAT	138:148	arg1	product					200:206	an ion exchange-purified product	175:206	an ion exchange-purified product derived from the water extract of virulent Shigella species	175:266	The native Invaplex (InvaplexNAT) vaccine and adjuvant is an ion exchange-purified product derived from the water extract of virulent Shigella species.
29600284	1	83	theme	InvaplexNAT	138:148	arg1	adjuvant					163:170	adjuvant	163:170	adjuvant	163:170	The native Invaplex (InvaplexNAT) vaccine and adjuvant is an ion exchange-purified product derived from the water extract of virulent Shigella species.
29600284	1	83	theme	InvaplexNAT	138:148	arg1	vaccine					151:157	The native Invaplex (InvaplexNAT) vaccine	117:157	The native Invaplex (InvaplexNAT) vaccine	117:157	The native Invaplex (InvaplexNAT) vaccine and adjuvant is an ion exchange-purified product derived from the water extract of virulent Shigella species.
29600284	12	84	theme	significant	1846:1856	arg1	advantages					1858:1867	significant advantages	1846:1867	significant advantages	1846:1867	The second-generation Shigella Invaplex vaccine, InvaplexAR, offers significant advantages over InvaplexNAT in reproducibility, flexible yet defined composition, immunogenicity, and protective efficacy.
29600284	4	85	theme	InvaplexAR	670:679	arg1	Characterization					650:665	Characterization	650:665	Characterization of InvaplexAR by various methods	650:698	Characterization of InvaplexAR by various methods demonstrated similar characteristics as the previously reported HMMC in InvaplexNAT.
29600284	13	86	theme	disease	2049:2055	arg1	worldwide					2057:2065	severe diarrheal disease worldwide	2032:2065	severe diarrheal disease worldwide	2032:2065	IMPORTANCEShigella species are bacteria that cause severe diarrheal disease worldwide, primarily in young children.
29600284	12	87	theme	Invaplex	1809:1816	arg1	InvaplexAR					1827:1836	InvaplexAR	1827:1836	InvaplexAR	1827:1836	The second-generation Shigella Invaplex vaccine, InvaplexAR, offers significant advantages over InvaplexNAT in reproducibility, flexible yet defined composition, immunogenicity, and protective efficacy.
29600284	12	87	theme	Invaplex	1809:1816	arg1	vaccine					1818:1824	The second-generation Shigella Invaplex vaccine	1778:1824	The second-generation Shigella Invaplex vaccine	1778:1824	The second-generation Shigella Invaplex vaccine, InvaplexAR, offers significant advantages over InvaplexNAT in reproducibility, flexible yet defined composition, immunogenicity, and protective efficacy.
29600284	17	88	theme	immunized	2708:2716	arg1	mice					2718:2721	immunized mice	2708:2721	immunized mice	2708:2721	The improved vaccine stimulates the production of antibodies in immunized mice and guinea pigs and protects these animals from Shigella infection.
29600284	4	89	theme	various	684:690	arg1	methods					692:698	various methods	684:698	various methods	684:698	Characterization of InvaplexAR by various methods demonstrated similar characteristics as the previously reported HMMC in InvaplexNAT.
29600284	0	90	theme	Artificial	96:105	arg1	Invaplex					107:114	Shigella Artificial Invaplex	87:114	Shigella Artificial Invaplex	87:114	Assembly, Biochemical Characterization, Immunogenicity, Adjuvanticity, and Efficacy of Shigella Artificial Invaplex.
29600284	10	91	theme	Guinea	1540:1545	arg1	pigs					1547:1550	Guinea pigs	1540:1550	Guinea pigs receiving approximately 5-fold less InvaplexAR compared to cohorts immunized with InvaplexNAT	1540:1644	Guinea pigs receiving approximately 5-fold less InvaplexAR compared to cohorts immunized with InvaplexNAT were protected from ocular challenge.
29600284	8	92	theme	InvaplexNAT	1398:1408	arg1	InvaplexNAT					1398:1408	InvaplexNAT	1398:1408	InvaplexNAT	1398:1408	In the murine model, homologous protection was achieved with 10-fold less InvaplexAR than InvaplexNAT and mice receiving InvaplexAR lost significantly less weight than mice receiving the same amount of InvaplexNAT.
29600284	8	92	theme	InvaplexNAT	1398:1408	arg1	amount					1388:1393	the same amount	1379:1393	the same amount of InvaplexNAT	1379:1408	In the murine model, homologous protection was achieved with 10-fold less InvaplexAR than InvaplexNAT and mice receiving InvaplexAR lost significantly less weight than mice receiving the same amount of InvaplexNAT.
29600284	13	93	theme	severe	2032:2037	arg1	worldwide					2057:2065	severe diarrheal disease worldwide	2032:2065	severe diarrheal disease worldwide	2032:2065	IMPORTANCEShigella species are bacteria that cause severe diarrheal disease worldwide, primarily in young children.
29600284	14	94	theme	high	2168:2171	arg1	burden					2173:2178	the high burden	2164:2178	the high burden of disease	2164:2189	Treatment of shigellosis includes oral fluids and antibiotics, but the high burden of disease, increasing prevalence of antibiotic resistance, and long-term health consequences clearly warrant the development of an effective vaccine.
29600284	12	95	theme	second-generation	1782:1798	arg1	InvaplexAR					1827:1836	InvaplexAR	1827:1836	InvaplexAR	1827:1836	The second-generation Shigella Invaplex vaccine, InvaplexAR, offers significant advantages over InvaplexNAT in reproducibility, flexible yet defined composition, immunogenicity, and protective efficacy.
29600284	12	95	theme	second-generation	1782:1798	arg1	vaccine					1818:1824	The second-generation Shigella Invaplex vaccine	1778:1824	The second-generation Shigella Invaplex vaccine	1778:1824	The second-generation Shigella Invaplex vaccine, InvaplexAR, offers significant advantages over InvaplexNAT in reproducibility, flexible yet defined composition, immunogenicity, and protective efficacy.
29600284	14	96	theme	health	2254:2259	arg1	consequences					2261:2272	long-term health consequences	2244:2272	long-term health consequences	2244:2272	Treatment of shigellosis includes oral fluids and antibiotics, but the high burden of disease, increasing prevalence of antibiotic resistance, and long-term health consequences clearly warrant the development of an effective vaccine.
29600284	13	97	theme	IMPORTANCEShigella	1981:1998	arg1	bacteria					2012:2019	bacteria	2012:2019	bacteria that cause severe diarrheal disease worldwide, primarily in young children	2012:2094	IMPORTANCEShigella species are bacteria that cause severe diarrheal disease worldwide, primarily in young children.
29600284	13	97	theme	IMPORTANCEShigella	1981:1998	arg1	species					2000:2006	IMPORTANCEShigella species	1981:2006	IMPORTANCEShigella species	1981:2006	IMPORTANCEShigella species are bacteria that cause severe diarrheal disease worldwide, primarily in young children.
29600284	4	98	theme	reported	755:762	arg1	HMMC					764:767	the previously reported HMMC	740:767	the previously reported HMMC in InvaplexNAT	740:782	Characterization of InvaplexAR by various methods demonstrated similar characteristics as the previously reported HMMC in InvaplexNAT.
29600284	4	98	theme	reported	755:762	arg1	characteristics					721:735	similar characteristics	713:735	similar characteristics	713:735	Characterization of InvaplexAR by various methods demonstrated similar characteristics as the previously reported HMMC in InvaplexNAT.
29600284	2	99	theme	invasin	402:408	arg1	IpaC					428:431	IpaC	428:431	IpaC	428:431	The key component of InvaplexNAT is a high-molecular-mass complex (HMMC) consisting of the Shigella lipopolysaccharide (LPS) and the invasin proteins IpaB and IpaC.
29600284	2	99	theme	invasin	402:408	arg1	IpaB					419:422	IpaB	419:422	IpaB	419:422	The key component of InvaplexNAT is a high-molecular-mass complex (HMMC) consisting of the Shigella lipopolysaccharide (LPS) and the invasin proteins IpaB and IpaC.
29600284	2	99	theme	invasin	402:408	arg1	proteins					410:417	the invasin proteins IpaB and IpaC	398:431	the invasin proteins IpaB and IpaC	398:431	The key component of InvaplexNAT is a high-molecular-mass complex (HMMC) consisting of the Shigella lipopolysaccharide (LPS) and the invasin proteins IpaB and IpaC.
29600284	5	100	theme	well-defined	789:800	arg1	vaccine					813:819	The well-defined InvaplexAR vaccine	785:819	The well-defined InvaplexAR vaccine	785:819	The well-defined InvaplexAR vaccine consistently contained greater quantities of IpaB, IpaC, and LPS than InvaplexNAT.
29600284	15	101	theme	immune	2440:2445	arg1	response					2447:2454	an immune response	2437:2454	an immune response to specific antigens of the bacteria	2437:2491	One Shigella vaccine under development is termed the invasin complex or Invaplex and is designed to drive an immune response to specific antigens of the bacteria in an effort to protect an individual from infection.
29600284	14	102	theme	resistance	2228:2237	arg1	consequences					2261:2272	long-term health consequences	2244:2272	long-term health consequences	2244:2272	Treatment of shigellosis includes oral fluids and antibiotics, but the high burden of disease, increasing prevalence of antibiotic resistance, and long-term health consequences clearly warrant the development of an effective vaccine.
29600284	14	102	theme	resistance	2228:2237	arg1	burden					2173:2178	the high burden	2164:2178	the high burden of disease	2164:2189	Treatment of shigellosis includes oral fluids and antibiotics, but the high burden of disease, increasing prevalence of antibiotic resistance, and long-term health consequences clearly warrant the development of an effective vaccine.
29600284	14	102	theme	resistance	2228:2237	arg1	prevalence					2203:2212	increasing prevalence	2192:2212	increasing prevalence of antibiotic resistance	2192:2237	Treatment of shigellosis includes oral fluids and antibiotics, but the high burden of disease, increasing prevalence of antibiotic resistance, and long-term health consequences clearly warrant the development of an effective vaccine.
29600284	3	103	theme	artificial	480:489	arg1	InvaplexAR					501:510	InvaplexAR	501:510	InvaplexAR	501:510	To improve product purity and immunogenicity, artificial Invaplex (InvaplexAR) was developed using recombinant IpaB and IpaC proteins and purified Shigella LPS to assemble an HMMC consisting of all three components.
29600284	3	103	theme	artificial	480:489	arg1	Invaplex					491:498	artificial Invaplex	480:498	artificial Invaplex (InvaplexAR)	480:511	To improve product purity and immunogenicity, artificial Invaplex (InvaplexAR) was developed using recombinant IpaB and IpaC proteins and purified Shigella LPS to assemble an HMMC consisting of all three components.
29600284	14	104	theme	increasing	2192:2201	arg1	prevalence					2203:2212	increasing prevalence	2192:2212	increasing prevalence of antibiotic resistance	2192:2237	Treatment of shigellosis includes oral fluids and antibiotics, but the high burden of disease, increasing prevalence of antibiotic resistance, and long-term health consequences clearly warrant the development of an effective vaccine.
29600284	1	105	theme	water	225:229	arg1	extract					231:237	the water extract	221:237	the water extract of virulent Shigella species	221:266	The native Invaplex (InvaplexNAT) vaccine and adjuvant is an ion exchange-purified product derived from the water extract of virulent Shigella species.
29331316	4	0	theme	paw	620:622	arg1	edema					624:628	carrageenan-induced paw edema	600:628	carrageenan-induced paw edema	600:628	Anti-inflammatory activity was also evaluated in vivo using carrageenan-induced paw edema in Wistar rats.
29331316	13	1	theme	traditional	1734:1744	arg1	use					1746:1748	the traditional use	1730:1748	the traditional use of the leaves of H. tuberculatum	1730:1781	CONCLUSION The results of the present study support the traditional use of the leaves of H. tuberculatum and may possibly serve as prospective material for further development of safe new phytochemical anti-inflammatory, gastroprotective and/or analgesic agents.
29331316	5	2	theme	HCl/ethanol-induced	693:711	arg1	test					727:730	the HCl/ethanol-induced gastric ulcer test	689:730	the HCl/ethanol-induced gastric ulcer test in rats	689:738	Gastroprotective activity was tested using the HCl/ethanol-induced gastric ulcer test in rats.
29331316	13	3	theme	phytochemical	1866:1878	arg1	agents					1933:1938	safe new phytochemical anti-inflammatory, gastroprotective and/or analgesic agents	1857:1938	safe new phytochemical anti-inflammatory, gastroprotective and/or analgesic agents	1857:1938	CONCLUSION The results of the present study support the traditional use of the leaves of H. tuberculatum and may possibly serve as prospective material for further development of safe new phytochemical anti-inflammatory, gastroprotective and/or analgesic agents.
29331316	3	4	theme	organic	522:528	arg1	extracts					530:537	the organic extracts	518:537	the organic extracts	518:537	MATERIALS AND METHODS Acute toxicity was studied in vivo to determine the toxic doses of the organic extracts.
29331316	5	5	theme	ulcer	721:725	arg1	test					727:730	the HCl/ethanol-induced gastric ulcer test	689:730	the HCl/ethanol-induced gastric ulcer test in rats	689:738	Gastroprotective activity was tested using the HCl/ethanol-induced gastric ulcer test in rats.
29331316	2	6	theme	study	310:314	arg1	aim					298:300	The aim	294:300	The aim of this study	294:314	The aim of this study is to investigate the organic extracts from H. tuberculatum leaves against inflammation, gastric ulcer and pain.
29331316	7	7	theme	acids	921:925	arg1	composition					896:906	The chemical composition	883:906	The chemical composition of the fatty acids in the petroleum ether (PE) extract	883:961	The chemical composition of the fatty acids in the petroleum ether (PE) extract was determined with GC-MS.
29331316	2	8	from	H.	360:361	arg1	extracts					346:353	the organic extracts	334:353	the organic extracts from H. tuberculatum leaves against inflammation, gastric ulcer and pain	334:426	The aim of this study is to investigate the organic extracts from H. tuberculatum leaves against inflammation, gastric ulcer and pain.
29331316	13	9	theme	present	1708:1714	arg1	study					1716:1720	the present study	1704:1720	the present study	1704:1720	CONCLUSION The results of the present study support the traditional use of the leaves of H. tuberculatum and may possibly serve as prospective material for further development of safe new phytochemical anti-inflammatory, gastroprotective and/or analgesic agents.
29331316	6	10	theme	hot-plate	851:859	arg1	method					861:866	the hot-plate method	847:866	the hot-plate method	847:866	Peripheral and central analgesic activities were assessed using the acetic acid-induced writhing test and the hot-plate method, respectively.
29331316	12	11	theme	GC-FID	1470:1475	arg1	analysis					1477:1484	The GC-FID analysis	1466:1484	The GC-FID analysis	1466:1484	The GC-FID analysis revealed that the PE extract was rich in γ-linolenic acid (45.50%) followed by palmitic acid (18.48%), linoleic acid (10.73%), erucic acid (4.72), stearic acid (3.96%) and oleic acid (2.57%).
29331316	11	12	theme	n-BuOH	1358:1363	arg1	extracts					1366:1373	butanolic (n-BuOH) extracts	1347:1373	butanolic (n-BuOH) extracts	1347:1373	The chloroform, ethyl acetate (EtOAc) and butanolic (n-BuOH) extracts exhibited strong anti-inflammatory, gastroprotective and analgesic activities at 100mg/kg.
29331316	13	13	theme	safe	1857:1860	arg1	agents					1933:1938	safe new phytochemical anti-inflammatory, gastroprotective and/or analgesic agents	1857:1938	safe new phytochemical anti-inflammatory, gastroprotective and/or analgesic agents	1857:1938	CONCLUSION The results of the present study support the traditional use of the leaves of H. tuberculatum and may possibly serve as prospective material for further development of safe new phytochemical anti-inflammatory, gastroprotective and/or analgesic agents.
29331316	1	14	theme	traditional	216:226	arg1	medicine					228:235	traditional medicine	216:235	traditional medicine	216:235	ETHNOPHARMACOLOGICAL RELEVANCE Haplophyllum tuberculatum is used in traditional medicine to treat many disorders including inflammation and pain.
29331316	12	15	from	acid	1539:1542	arg1	rich					1519:1522	rich	1519:1522	rich	1519:1522	The GC-FID analysis revealed that the PE extract was rich in γ-linolenic acid (45.50%) followed by palmitic acid (18.48%), linoleic acid (10.73%), erucic acid (4.72), stearic acid (3.96%) and oleic acid (2.57%).
29331316	12	15	from	acid	1539:1542	arg1	followed					1553:1560	followed	1553:1560	followed by palmitic acid (18.48%), linoleic acid (10.73%), erucic acid (4.72), stearic acid (3.96%) and oleic acid (2.57%)	1553:1675	The GC-FID analysis revealed that the PE extract was rich in γ-linolenic acid (45.50%) followed by palmitic acid (18.48%), linoleic acid (10.73%), erucic acid (4.72), stearic acid (3.96%) and oleic acid (2.57%).
29331316	12	15	from	acid	1539:1542	arg1	extract					1507:1513	the PE extract	1500:1513	the PE extract	1500:1513	The GC-FID analysis revealed that the PE extract was rich in γ-linolenic acid (45.50%) followed by palmitic acid (18.48%), linoleic acid (10.73%), erucic acid (4.72), stearic acid (3.96%) and oleic acid (2.57%).
29331316	11	16	theme	butanolic	1347:1355	arg1	extracts					1366:1373	butanolic (n-BuOH) extracts	1347:1373	butanolic (n-BuOH) extracts	1347:1373	The chloroform, ethyl acetate (EtOAc) and butanolic (n-BuOH) extracts exhibited strong anti-inflammatory, gastroprotective and analgesic activities at 100mg/kg.
29331316	10	17	from	100mg/kg	1193:1200	arg1	extract					1182:1188	The same extract	1173:1188	The same extract at 100mg/kg	1173:1200	The same extract at 100mg/kg showed good analgesic activities using the acetic acid-induced writhing test and the hot-plate method.
29331316	11	18	theme	strong	1385:1390	arg1	activities					1442:1451	strong anti-inflammatory, gastroprotective and analgesic activities	1385:1451	strong anti-inflammatory, gastroprotective and analgesic activities	1385:1451	The chloroform, ethyl acetate (EtOAc) and butanolic (n-BuOH) extracts exhibited strong anti-inflammatory, gastroprotective and analgesic activities at 100mg/kg.
29331316	1	19	theme	many	246:249	arg1	inflammation					271:282	inflammation	271:282	inflammation	271:282	ETHNOPHARMACOLOGICAL RELEVANCE Haplophyllum tuberculatum is used in traditional medicine to treat many disorders including inflammation and pain.
29331316	1	19	theme	many	246:249	arg1	disorders					251:259	many disorders	246:259	many disorders including inflammation and pain	246:291	ETHNOPHARMACOLOGICAL RELEVANCE Haplophyllum tuberculatum is used in traditional medicine to treat many disorders including inflammation and pain.
29331316	1	19	theme	many	246:249	arg1	pain					288:291	pain	288:291	pain	288:291	ETHNOPHARMACOLOGICAL RELEVANCE Haplophyllum tuberculatum is used in traditional medicine to treat many disorders including inflammation and pain.
29331316	12	20	theme	oleic	1658:1662	arg1	acid					1664:1667	oleic acid	1658:1667	oleic acid (2.57%)	1658:1675	The GC-FID analysis revealed that the PE extract was rich in γ-linolenic acid (45.50%) followed by palmitic acid (18.48%), linoleic acid (10.73%), erucic acid (4.72), stearic acid (3.96%) and oleic acid (2.57%).
29331316	12	20	theme	oleic	1658:1662	arg1	%					1674:1674	2.57%	1670:1674	2.57%	1670:1674	The GC-FID analysis revealed that the PE extract was rich in γ-linolenic acid (45.50%) followed by palmitic acid (18.48%), linoleic acid (10.73%), erucic acid (4.72), stearic acid (3.96%) and oleic acid (2.57%).
29331316	12	21	theme	palmitic	1565:1572	arg1	acid					1574:1577	palmitic acid	1565:1577	palmitic acid (18.48%)	1565:1586	The GC-FID analysis revealed that the PE extract was rich in γ-linolenic acid (45.50%) followed by palmitic acid (18.48%), linoleic acid (10.73%), erucic acid (4.72), stearic acid (3.96%) and oleic acid (2.57%).
29331316	12	21	theme	palmitic	1565:1572	arg1	%					1585:1585	18.48%	1580:1585	18.48%	1580:1585	The GC-FID analysis revealed that the PE extract was rich in γ-linolenic acid (45.50%) followed by palmitic acid (18.48%), linoleic acid (10.73%), erucic acid (4.72), stearic acid (3.96%) and oleic acid (2.57%).
29331316	1	22	used	used	208:211	arg2	RELEVANCE					169:177	ETHNOPHARMACOLOGICAL RELEVANCE	148:177	ETHNOPHARMACOLOGICAL RELEVANCE Haplophyllum tuberculatum	148:203	ETHNOPHARMACOLOGICAL RELEVANCE Haplophyllum tuberculatum is used in traditional medicine to treat many disorders including inflammation and pain.
29331316	10	23	theme	acid-induced	1252:1263	arg1	test					1274:1277	the acetic acid-induced writhing test	1241:1277	the acetic acid-induced writhing test	1241:1277	The same extract at 100mg/kg showed good analgesic activities using the acetic acid-induced writhing test and the hot-plate method.
29331316	0	24	theme	acid	61:64	arg1	composition					66:76	fatty acid composition	55:76	fatty acid composition of the petroleum ether extract from Haplophyllum tuberculatum	55:138	Pharmacological activities of the organic extracts and fatty acid composition of the petroleum ether extract from Haplophyllum tuberculatum leaves.
29331316	9	25	theme	inhibition	1086:1095	arg1	5h					1097:1098	inhibition 5h	1086:1098	inhibition 5h	1086:1098	Percentages inhibition 5h after carrageenan-injection were 51.12; 86.71% and 96.92%, respectively.
29331316	0	26	theme	petroleum	85:93	arg1	extract					101:107	the petroleum ether extract	81:107	the petroleum ether extract from Haplophyllum tuberculatum	81:138	Pharmacological activities of the organic extracts and fatty acid composition of the petroleum ether extract from Haplophyllum tuberculatum leaves.
29331316	6	27	theme	central	756:762	arg1	activities					774:783	Peripheral and central analgesic activities	741:783	Peripheral and central analgesic activities	741:783	Peripheral and central analgesic activities were assessed using the acetic acid-induced writhing test and the hot-plate method, respectively.
29331316	0	28	theme	extract	101:107	arg1	activities					16:25	Pharmacological activities	0:25	Pharmacological activities of the organic extracts	0:49	Pharmacological activities of the organic extracts and fatty acid composition of the petroleum ether extract from Haplophyllum tuberculatum leaves.
29331316	0	28	theme	extract	101:107	arg1	composition					66:76	fatty acid composition	55:76	fatty acid composition of the petroleum ether extract from Haplophyllum tuberculatum	55:138	Pharmacological activities of the organic extracts and fatty acid composition of the petroleum ether extract from Haplophyllum tuberculatum leaves.
29331316	1	29	dep	Haplophyllum	179:190	arg1	tuberculatum					192:203	tuberculatum	192:203	tuberculatum	192:203	ETHNOPHARMACOLOGICAL RELEVANCE Haplophyllum tuberculatum is used in traditional medicine to treat many disorders including inflammation and pain.
29331316	6	30	theme	Peripheral	741:750	arg1	activities					774:783	Peripheral and central analgesic activities	741:783	Peripheral and central analgesic activities	741:783	Peripheral and central analgesic activities were assessed using the acetic acid-induced writhing test and the hot-plate method, respectively.
29331316	3	31	theme	Acute	451:455	arg1	toxicity					457:464	MATERIALS AND METHODS Acute toxicity	429:464	MATERIALS AND METHODS Acute toxicity	429:464	MATERIALS AND METHODS Acute toxicity was studied in vivo to determine the toxic doses of the organic extracts.
29331316	0	32	theme	Pharmacological	0:14	arg1	activities					16:25	Pharmacological activities	0:25	Pharmacological activities of the organic extracts	0:49	Pharmacological activities of the organic extracts and fatty acid composition of the petroleum ether extract from Haplophyllum tuberculatum leaves.
29331316	8	33	theme	100mg/kg	1012:1019	arg1	extract					1024:1030	100mg/kg PE extract	1012:1030	100mg/kg PE extract	1012:1030	RESULTS At 25, 50 and 100mg/kg PE extract was the most active against inflammation.
29331316	8	34	from	25	1001:1002	arg1	active					1045:1050	active	1045:1050	active	1045:1050	RESULTS At 25, 50 and 100mg/kg PE extract was the most active against inflammation.
29331316	8	34	from	25	1001:1002	arg1	RESULTS					990:996	RESULTS	990:996	RESULTS At 25, 50 and 100mg/kg PE extract	990:1030	RESULTS At 25, 50 and 100mg/kg PE extract was the most active against inflammation.
29331316	13	35	theme	further	1834:1840	arg1	development					1842:1852	further development	1834:1852	further development of safe new phytochemical anti-inflammatory, gastroprotective and/or analgesic agents	1834:1938	CONCLUSION The results of the present study support the traditional use of the leaves of H. tuberculatum and may possibly serve as prospective material for further development of safe new phytochemical anti-inflammatory, gastroprotective and/or analgesic agents.
29331316	0	36	theme	organic	34:40	arg1	extracts					42:49	the organic extracts	30:49	the organic extracts	30:49	Pharmacological activities of the organic extracts and fatty acid composition of the petroleum ether extract from Haplophyllum tuberculatum leaves.
29331316	4	37	theme	Anti-inflammatory	540:556	arg1	activity					558:565	Anti-inflammatory activity	540:565	Anti-inflammatory activity	540:565	Anti-inflammatory activity was also evaluated in vivo using carrageenan-induced paw edema in Wistar rats.
29331316	7	38	from	composition	896:906	arg1	extract					955:961	the petroleum ether (PE) extract	930:961	the petroleum ether (PE) extract	930:961	The chemical composition of the fatty acids in the petroleum ether (PE) extract was determined with GC-MS.
29331316	6	39	theme	acid-induced	816:827	arg1	test					838:841	the acetic acid-induced writhing test	805:841	the acetic acid-induced writhing test	805:841	Peripheral and central analgesic activities were assessed using the acetic acid-induced writhing test and the hot-plate method, respectively.
29331316	10	40	theme	same	1177:1180	arg1	extract					1182:1188	The same extract	1173:1188	The same extract at 100mg/kg	1173:1200	The same extract at 100mg/kg showed good analgesic activities using the acetic acid-induced writhing test and the hot-plate method.
29331316	10	41	theme	analgesic	1214:1222	arg1	activities					1224:1233	good analgesic activities	1209:1233	good analgesic activities using the acetic acid-induced writhing test	1209:1277	The same extract at 100mg/kg showed good analgesic activities using the acetic acid-induced writhing test and the hot-plate method.
29331316	13	42	dep	the	1753:1755	arg1	leaves					1757:1762	leaves	1757:1762	leaves	1757:1762	CONCLUSION The results of the present study support the traditional use of the leaves of H. tuberculatum and may possibly serve as prospective material for further development of safe new phytochemical anti-inflammatory, gastroprotective and/or analgesic agents.
29331316	8	43	from	50	1005:1006	arg1	active					1045:1050	active	1045:1050	active	1045:1050	RESULTS At 25, 50 and 100mg/kg PE extract was the most active against inflammation.
29331316	8	43	from	50	1005:1006	arg1	RESULTS					990:996	RESULTS	990:996	RESULTS At 25, 50 and 100mg/kg PE extract	990:1030	RESULTS At 25, 50 and 100mg/kg PE extract was the most active against inflammation.
29331316	4	44	theme	Wistar	633:638	arg1	rats					640:643	Wistar rats	633:643	Wistar rats	633:643	Anti-inflammatory activity was also evaluated in vivo using carrageenan-induced paw edema in Wistar rats.
29331316	11	45	theme	gastroprotective	1411:1426	arg1	activities					1442:1451	strong anti-inflammatory, gastroprotective and analgesic activities	1385:1451	strong anti-inflammatory, gastroprotective and analgesic activities	1385:1451	The chloroform, ethyl acetate (EtOAc) and butanolic (n-BuOH) extracts exhibited strong anti-inflammatory, gastroprotective and analgesic activities at 100mg/kg.
29331316	13	46	theme	analgesic	1923:1931	arg1	agents					1933:1938	safe new phytochemical anti-inflammatory, gastroprotective and/or analgesic agents	1857:1938	safe new phytochemical anti-inflammatory, gastroprotective and/or analgesic agents	1857:1938	CONCLUSION The results of the present study support the traditional use of the leaves of H. tuberculatum and may possibly serve as prospective material for further development of safe new phytochemical anti-inflammatory, gastroprotective and/or analgesic agents.
29331316	3	47	theme	extracts	530:537	arg1	doses					509:513	the toxic doses	499:513	the toxic doses of the organic extracts	499:537	MATERIALS AND METHODS Acute toxicity was studied in vivo to determine the toxic doses of the organic extracts.
29331316	5	48	from	test	727:730	arg1	rats					735:738	rats	735:738	rats	735:738	Gastroprotective activity was tested using the HCl/ethanol-induced gastric ulcer test in rats.
29331316	11	49	theme	anti-inflammatory	1392:1408	arg1	activities					1442:1451	strong anti-inflammatory, gastroprotective and analgesic activities	1385:1451	strong anti-inflammatory, gastroprotective and analgesic activities	1385:1451	The chloroform, ethyl acetate (EtOAc) and butanolic (n-BuOH) extracts exhibited strong anti-inflammatory, gastroprotective and analgesic activities at 100mg/kg.
29331316	10	50	theme	acetic	1245:1250	arg1	test					1274:1277	the acetic acid-induced writhing test	1241:1277	the acetic acid-induced writhing test	1241:1277	The same extract at 100mg/kg showed good analgesic activities using the acetic acid-induced writhing test and the hot-plate method.
29331316	12	51	theme	PE	1504:1505	arg1	followed					1553:1560	followed	1553:1560	followed by palmitic acid (18.48%), linoleic acid (10.73%), erucic acid (4.72), stearic acid (3.96%) and oleic acid (2.57%)	1553:1675	The GC-FID analysis revealed that the PE extract was rich in γ-linolenic acid (45.50%) followed by palmitic acid (18.48%), linoleic acid (10.73%), erucic acid (4.72), stearic acid (3.96%) and oleic acid (2.57%).
29331316	12	51	theme	PE	1504:1505	arg1	rich					1519:1522	rich	1519:1522	rich	1519:1522	The GC-FID analysis revealed that the PE extract was rich in γ-linolenic acid (45.50%) followed by palmitic acid (18.48%), linoleic acid (10.73%), erucic acid (4.72), stearic acid (3.96%) and oleic acid (2.57%).
29331316	12	51	theme	PE	1504:1505	arg1	extract					1507:1513	the PE extract	1500:1513	the PE extract	1500:1513	The GC-FID analysis revealed that the PE extract was rich in γ-linolenic acid (45.50%) followed by palmitic acid (18.48%), linoleic acid (10.73%), erucic acid (4.72), stearic acid (3.96%) and oleic acid (2.57%).
29331316	8	52	from	extract	1024:1030	arg1	active					1045:1050	active	1045:1050	active	1045:1050	RESULTS At 25, 50 and 100mg/kg PE extract was the most active against inflammation.
29331316	8	52	from	extract	1024:1030	arg1	RESULTS					990:996	RESULTS	990:996	RESULTS At 25, 50 and 100mg/kg PE extract	990:1030	RESULTS At 25, 50 and 100mg/kg PE extract was the most active against inflammation.
29331316	4	53	theme	carrageenan-induced	600:618	arg1	edema					624:628	carrageenan-induced paw edema	600:628	carrageenan-induced paw edema	600:628	Anti-inflammatory activity was also evaluated in vivo using carrageenan-induced paw edema in Wistar rats.
29331316	5	54	theme	gastric	713:719	arg1	test					727:730	the HCl/ethanol-induced gastric ulcer test	689:730	the HCl/ethanol-induced gastric ulcer test in rats	689:738	Gastroprotective activity was tested using the HCl/ethanol-induced gastric ulcer test in rats.
29331316	13	55	theme	anti-inflammatory	1880:1896	arg1	agents					1933:1938	safe new phytochemical anti-inflammatory, gastroprotective and/or analgesic agents	1857:1938	safe new phytochemical anti-inflammatory, gastroprotective and/or analgesic agents	1857:1938	CONCLUSION The results of the present study support the traditional use of the leaves of H. tuberculatum and may possibly serve as prospective material for further development of safe new phytochemical anti-inflammatory, gastroprotective and/or analgesic agents.
29331316	7	56	theme	petroleum	934:942	arg1	extract					955:961	the petroleum ether (PE) extract	930:961	the petroleum ether (PE) extract	930:961	The chemical composition of the fatty acids in the petroleum ether (PE) extract was determined with GC-MS.
29331316	8	57	theme	PE	1021:1022	arg1	extract					1024:1030	100mg/kg PE extract	1012:1030	100mg/kg PE extract	1012:1030	RESULTS At 25, 50 and 100mg/kg PE extract was the most active against inflammation.
29331316	11	58	theme	analgesic	1432:1440	arg1	activities					1442:1451	strong anti-inflammatory, gastroprotective and analgesic activities	1385:1451	strong anti-inflammatory, gastroprotective and analgesic activities	1385:1451	The chloroform, ethyl acetate (EtOAc) and butanolic (n-BuOH) extracts exhibited strong anti-inflammatory, gastroprotective and analgesic activities at 100mg/kg.
29331316	13	59	theme	gastroprotective	1899:1914	arg1	agents					1933:1938	safe new phytochemical anti-inflammatory, gastroprotective and/or analgesic agents	1857:1938	safe new phytochemical anti-inflammatory, gastroprotective and/or analgesic agents	1857:1938	CONCLUSION The results of the present study support the traditional use of the leaves of H. tuberculatum and may possibly serve as prospective material for further development of safe new phytochemical anti-inflammatory, gastroprotective and/or analgesic agents.
29331316	9	60	dep	5h	1097:1098	arg1	carrageenan-injection					1106:1126	carrageenan-injection	1106:1126	carrageenan-injection	1106:1126	Percentages inhibition 5h after carrageenan-injection were 51.12; 86.71% and 96.92%, respectively.
29331316	2	61	theme	organic	338:344	arg1	extracts					346:353	the organic extracts	334:353	the organic extracts from H. tuberculatum leaves against inflammation, gastric ulcer and pain	334:426	The aim of this study is to investigate the organic extracts from H. tuberculatum leaves against inflammation, gastric ulcer and pain.
29331316	7	62	theme	fatty	915:919	arg1	acids					921:925	the fatty acids	911:925	the fatty acids	911:925	The chemical composition of the fatty acids in the petroleum ether (PE) extract was determined with GC-MS.
29331316	13	63	theme	new	1862:1864	arg1	agents					1933:1938	safe new phytochemical anti-inflammatory, gastroprotective and/or analgesic agents	1857:1938	safe new phytochemical anti-inflammatory, gastroprotective and/or analgesic agents	1857:1938	CONCLUSION The results of the present study support the traditional use of the leaves of H. tuberculatum and may possibly serve as prospective material for further development of safe new phytochemical anti-inflammatory, gastroprotective and/or analgesic agents.
29331316	7	64	theme	chemical	887:894	arg1	composition					896:906	The chemical composition	883:906	The chemical composition of the fatty acids in the petroleum ether (PE) extract	883:961	The chemical composition of the fatty acids in the petroleum ether (PE) extract was determined with GC-MS.
29331316	12	65	from	rich	1519:1522	arg1	acid					1539:1542	γ-linolenic acid	1527:1542	γ-linolenic acid (45.50%)	1527:1551	The GC-FID analysis revealed that the PE extract was rich in γ-linolenic acid (45.50%) followed by palmitic acid (18.48%), linoleic acid (10.73%), erucic acid (4.72), stearic acid (3.96%) and oleic acid (2.57%).
29331316	12	65	from	rich	1519:1522	arg1	%					1550:1550	45.50%	1545:1550	45.50%	1545:1550	The GC-FID analysis revealed that the PE extract was rich in γ-linolenic acid (45.50%) followed by palmitic acid (18.48%), linoleic acid (10.73%), erucic acid (4.72), stearic acid (3.96%) and oleic acid (2.57%).
29331316	0	66	from	composition	66:76	arg1	tuberculatum					127:138	Haplophyllum tuberculatum	114:138	Haplophyllum tuberculatum	114:138	Pharmacological activities of the organic extracts and fatty acid composition of the petroleum ether extract from Haplophyllum tuberculatum leaves.
29331316	12	67	theme	linoleic	1589:1596	arg1	acid					1598:1601	linoleic acid	1589:1601	linoleic acid (10.73%)	1589:1610	The GC-FID analysis revealed that the PE extract was rich in γ-linolenic acid (45.50%) followed by palmitic acid (18.48%), linoleic acid (10.73%), erucic acid (4.72), stearic acid (3.96%) and oleic acid (2.57%).
29331316	12	67	theme	linoleic	1589:1596	arg1	%					1609:1609	10.73%	1604:1609	10.73%	1604:1609	The GC-FID analysis revealed that the PE extract was rich in γ-linolenic acid (45.50%) followed by palmitic acid (18.48%), linoleic acid (10.73%), erucic acid (4.72), stearic acid (3.96%) and oleic acid (2.57%).
29331316	13	68	theme	prospective	1809:1819	arg1	material					1821:1828	prospective material	1809:1828	prospective material for further development of safe new phytochemical anti-inflammatory, gastroprotective and/or analgesic agents	1809:1938	CONCLUSION The results of the present study support the traditional use of the leaves of H. tuberculatum and may possibly serve as prospective material for further development of safe new phytochemical anti-inflammatory, gastroprotective and/or analgesic agents.
29331316	10	69	theme	writhing	1265:1272	arg1	test					1274:1277	the acetic acid-induced writhing test	1241:1277	the acetic acid-induced writhing test	1241:1277	The same extract at 100mg/kg showed good analgesic activities using the acetic acid-induced writhing test and the hot-plate method.
29331316	12	70	dep	rich	1519:1522	arg1	followed					1553:1560	followed	1553:1560	followed by palmitic acid (18.48%), linoleic acid (10.73%), erucic acid (4.72), stearic acid (3.96%) and oleic acid (2.57%)	1553:1675	The GC-FID analysis revealed that the PE extract was rich in γ-linolenic acid (45.50%) followed by palmitic acid (18.48%), linoleic acid (10.73%), erucic acid (4.72), stearic acid (3.96%) and oleic acid (2.57%).
29331316	12	70	dep	rich	1519:1522	arg1	rich					1519:1522	rich	1519:1522	rich	1519:1522	The GC-FID analysis revealed that the PE extract was rich in γ-linolenic acid (45.50%) followed by palmitic acid (18.48%), linoleic acid (10.73%), erucic acid (4.72), stearic acid (3.96%) and oleic acid (2.57%).
29331316	12	70	dep	rich	1519:1522	arg1	extract					1507:1513	the PE extract	1500:1513	the PE extract	1500:1513	The GC-FID analysis revealed that the PE extract was rich in γ-linolenic acid (45.50%) followed by palmitic acid (18.48%), linoleic acid (10.73%), erucic acid (4.72), stearic acid (3.96%) and oleic acid (2.57%).
29331316	1	71	theme	ETHNOPHARMACOLOGICAL	148:167	arg1	RELEVANCE					169:177	ETHNOPHARMACOLOGICAL RELEVANCE	148:177	ETHNOPHARMACOLOGICAL RELEVANCE Haplophyllum tuberculatum	148:203	ETHNOPHARMACOLOGICAL RELEVANCE Haplophyllum tuberculatum is used in traditional medicine to treat many disorders including inflammation and pain.
29331316	13	72	dep	CONCLUSION	1678:1687	arg1	serve					1800:1804	serve	1800:1804	may possibly serve as prospective material for further development of safe new phytochemical anti-inflammatory, gastroprotective and/or analgesic agents	1787:1938	CONCLUSION The results of the present study support the traditional use of the leaves of H. tuberculatum and may possibly serve as prospective material for further development of safe new phytochemical anti-inflammatory, gastroprotective and/or analgesic agents.
29331316	13	72	dep	CONCLUSION	1678:1687	arg1	support					1722:1728	support	1722:1728	support the traditional use of the leaves of H. tuberculatum	1722:1781	CONCLUSION The results of the present study support the traditional use of the leaves of H. tuberculatum and may possibly serve as prospective material for further development of safe new phytochemical anti-inflammatory, gastroprotective and/or analgesic agents.
29331316	1	73	theme	Haplophyllum	179:190	arg1	RELEVANCE					169:177	ETHNOPHARMACOLOGICAL RELEVANCE	148:177	ETHNOPHARMACOLOGICAL RELEVANCE Haplophyllum tuberculatum	148:203	ETHNOPHARMACOLOGICAL RELEVANCE Haplophyllum tuberculatum is used in traditional medicine to treat many disorders including inflammation and pain.
29331316	12	74	theme	stearic	1633:1639	arg1	acid					1641:1644	stearic acid	1633:1644	stearic acid (3.96%)	1633:1652	The GC-FID analysis revealed that the PE extract was rich in γ-linolenic acid (45.50%) followed by palmitic acid (18.48%), linoleic acid (10.73%), erucic acid (4.72), stearic acid (3.96%) and oleic acid (2.57%).
29331316	12	74	theme	stearic	1633:1639	arg1	%					1651:1651	3.96%	1647:1651	3.96%	1647:1651	The GC-FID analysis revealed that the PE extract was rich in γ-linolenic acid (45.50%) followed by palmitic acid (18.48%), linoleic acid (10.73%), erucic acid (4.72), stearic acid (3.96%) and oleic acid (2.57%).
29331316	0	75	from	activities	16:25	arg1	tuberculatum					127:138	Haplophyllum tuberculatum	114:138	Haplophyllum tuberculatum	114:138	Pharmacological activities of the organic extracts and fatty acid composition of the petroleum ether extract from Haplophyllum tuberculatum leaves.
29331316	11	76	theme	ethyl	1321:1325	arg1	EtOAc					1336:1340	EtOAc	1336:1340	EtOAc	1336:1340	The chloroform, ethyl acetate (EtOAc) and butanolic (n-BuOH) extracts exhibited strong anti-inflammatory, gastroprotective and analgesic activities at 100mg/kg.
29331316	11	76	theme	ethyl	1321:1325	arg1	acetate					1327:1333	ethyl acetate	1321:1333	ethyl acetate (EtOAc)	1321:1341	The chloroform, ethyl acetate (EtOAc) and butanolic (n-BuOH) extracts exhibited strong anti-inflammatory, gastroprotective and analgesic activities at 100mg/kg.
29331316	10	77	theme	hot-plate	1287:1295	arg1	method					1297:1302	the hot-plate method	1283:1302	the hot-plate method	1283:1302	The same extract at 100mg/kg showed good analgesic activities using the acetic acid-induced writhing test and the hot-plate method.
29331316	0	78	theme	ether	95:99	arg1	extract					101:107	the petroleum ether extract	81:107	the petroleum ether extract from Haplophyllum tuberculatum	81:138	Pharmacological activities of the organic extracts and fatty acid composition of the petroleum ether extract from Haplophyllum tuberculatum leaves.
29331316	6	79	theme	analgesic	764:772	arg1	activities					774:783	Peripheral and central analgesic activities	741:783	Peripheral and central analgesic activities	741:783	Peripheral and central analgesic activities were assessed using the acetic acid-induced writhing test and the hot-plate method, respectively.
29331316	5	80	theme	Gastroprotective	646:661	arg1	activity					663:670	Gastroprotective activity	646:670	Gastroprotective activity	646:670	Gastroprotective activity was tested using the HCl/ethanol-induced gastric ulcer test in rats.
29331316	13	81	theme	study	1716:1720	arg1	results					1693:1699	The results	1689:1699	The results of the present study	1689:1720	CONCLUSION The results of the present study support the traditional use of the leaves of H. tuberculatum and may possibly serve as prospective material for further development of safe new phytochemical anti-inflammatory, gastroprotective and/or analgesic agents.
29331316	2	82	theme	gastric	405:411	arg1	ulcer					413:417	gastric ulcer	405:417	gastric ulcer	405:417	The aim of this study is to investigate the organic extracts from H. tuberculatum leaves against inflammation, gastric ulcer and pain.
29331316	2	83	dep	H.	360:361	arg1	tuberculatum					363:374	H. tuberculatum	360:374	H. tuberculatum leaves against inflammation, gastric ulcer and pain	360:426	The aim of this study is to investigate the organic extracts from H. tuberculatum leaves against inflammation, gastric ulcer and pain.
29331316	2	83	dep	H.	360:361	arg1	leaves					376:381	leaves	376:381	leaves against inflammation, gastric ulcer and pain	376:426	The aim of this study is to investigate the organic extracts from H. tuberculatum leaves against inflammation, gastric ulcer and pain.
29331316	13	84	theme	the	1753:1755	arg1	use					1746:1748	the traditional use	1730:1748	the traditional use of the leaves of H. tuberculatum	1730:1781	CONCLUSION The results of the present study support the traditional use of the leaves of H. tuberculatum and may possibly serve as prospective material for further development of safe new phytochemical anti-inflammatory, gastroprotective and/or analgesic agents.
29331316	0	85	theme	extracts	42:49	arg1	activities					16:25	Pharmacological activities	0:25	Pharmacological activities of the organic extracts	0:49	Pharmacological activities of the organic extracts and fatty acid composition of the petroleum ether extract from Haplophyllum tuberculatum leaves.
29331316	0	85	theme	extracts	42:49	arg1	composition					66:76	fatty acid composition	55:76	fatty acid composition of the petroleum ether extract from Haplophyllum tuberculatum	55:138	Pharmacological activities of the organic extracts and fatty acid composition of the petroleum ether extract from Haplophyllum tuberculatum leaves.
29331316	13	86	theme	H.	1767:1768	arg1	tuberculatum					1770:1781	H. tuberculatum	1767:1781	H. tuberculatum	1767:1781	CONCLUSION The results of the present study support the traditional use of the leaves of H. tuberculatum and may possibly serve as prospective material for further development of safe new phytochemical anti-inflammatory, gastroprotective and/or analgesic agents.
29331316	12	87	theme	γ-linolenic	1527:1537	arg1	acid					1539:1542	γ-linolenic acid	1527:1542	γ-linolenic acid (45.50%)	1527:1551	The GC-FID analysis revealed that the PE extract was rich in γ-linolenic acid (45.50%) followed by palmitic acid (18.48%), linoleic acid (10.73%), erucic acid (4.72), stearic acid (3.96%) and oleic acid (2.57%).
29331316	12	87	theme	γ-linolenic	1527:1537	arg1	%					1550:1550	45.50%	1545:1550	45.50%	1545:1550	The GC-FID analysis revealed that the PE extract was rich in γ-linolenic acid (45.50%) followed by palmitic acid (18.48%), linoleic acid (10.73%), erucic acid (4.72), stearic acid (3.96%) and oleic acid (2.57%).
29331316	12	88	theme	erucic	1613:1618	arg1	4.72					1626:1629	4.72	1626:1629	4.72	1626:1629	The GC-FID analysis revealed that the PE extract was rich in γ-linolenic acid (45.50%) followed by palmitic acid (18.48%), linoleic acid (10.73%), erucic acid (4.72), stearic acid (3.96%) and oleic acid (2.57%).
29331316	12	88	theme	erucic	1613:1618	arg1	acid					1620:1623	erucic acid	1613:1623	erucic acid (4.72)	1613:1630	The GC-FID analysis revealed that the PE extract was rich in γ-linolenic acid (45.50%) followed by palmitic acid (18.48%), linoleic acid (10.73%), erucic acid (4.72), stearic acid (3.96%) and oleic acid (2.57%).
29331316	3	89	theme	METHODS	443:449	arg1	toxicity					457:464	MATERIALS AND METHODS Acute toxicity	429:464	MATERIALS AND METHODS Acute toxicity	429:464	MATERIALS AND METHODS Acute toxicity was studied in vivo to determine the toxic doses of the organic extracts.
29331316	6	90	theme	writhing	829:836	arg1	test					838:841	the acetic acid-induced writhing test	805:841	the acetic acid-induced writhing test	805:841	Peripheral and central analgesic activities were assessed using the acetic acid-induced writhing test and the hot-plate method, respectively.
29331316	0	91	theme	fatty	55:59	arg1	composition					66:76	fatty acid composition	55:76	fatty acid composition of the petroleum ether extract from Haplophyllum tuberculatum	55:138	Pharmacological activities of the organic extracts and fatty acid composition of the petroleum ether extract from Haplophyllum tuberculatum leaves.
29331316	3	92	theme	MATERIALS	429:437	arg1	toxicity					457:464	MATERIALS AND METHODS Acute toxicity	429:464	MATERIALS AND METHODS Acute toxicity	429:464	MATERIALS AND METHODS Acute toxicity was studied in vivo to determine the toxic doses of the organic extracts.
29331316	6	93	theme	acetic	809:814	arg1	test					838:841	the acetic acid-induced writhing test	805:841	the acetic acid-induced writhing test	805:841	Peripheral and central analgesic activities were assessed using the acetic acid-induced writhing test and the hot-plate method, respectively.
29331316	3	94	theme	toxic	503:507	arg1	doses					509:513	the toxic doses	499:513	the toxic doses of the organic extracts	499:537	MATERIALS AND METHODS Acute toxicity was studied in vivo to determine the toxic doses of the organic extracts.
29331316	7	95	theme	PE	951:952	arg1	extract					955:961	the petroleum ether (PE) extract	930:961	the petroleum ether (PE) extract	930:961	The chemical composition of the fatty acids in the petroleum ether (PE) extract was determined with GC-MS.
29331316	10	96	theme	good	1209:1212	arg1	activities					1224:1233	good analgesic activities	1209:1233	good analgesic activities using the acetic acid-induced writhing test	1209:1277	The same extract at 100mg/kg showed good analgesic activities using the acetic acid-induced writhing test and the hot-plate method.
29331316	0	97	from	tuberculatum	127:138	arg1	extract					101:107	the petroleum ether extract	81:107	the petroleum ether extract from Haplophyllum tuberculatum	81:138	Pharmacological activities of the organic extracts and fatty acid composition of the petroleum ether extract from Haplophyllum tuberculatum leaves.
29331316	0	97	from	tuberculatum	127:138	arg1	activities					16:25	Pharmacological activities	0:25	Pharmacological activities of the organic extracts	0:49	Pharmacological activities of the organic extracts and fatty acid composition of the petroleum ether extract from Haplophyllum tuberculatum leaves.
29331316	0	97	from	tuberculatum	127:138	arg1	composition					66:76	fatty acid composition	55:76	fatty acid composition of the petroleum ether extract from Haplophyllum tuberculatum	55:138	Pharmacological activities of the organic extracts and fatty acid composition of the petroleum ether extract from Haplophyllum tuberculatum leaves.
29331316	13	98	theme	agents	1933:1938	arg1	development					1842:1852	further development	1834:1852	further development of safe new phytochemical anti-inflammatory, gastroprotective and/or analgesic agents	1834:1938	CONCLUSION The results of the present study support the traditional use of the leaves of H. tuberculatum and may possibly serve as prospective material for further development of safe new phytochemical anti-inflammatory, gastroprotective and/or analgesic agents.
29331316	7	99	theme	ether	944:948	arg1	extract					955:961	the petroleum ether (PE) extract	930:961	the petroleum ether (PE) extract	930:961	The chemical composition of the fatty acids in the petroleum ether (PE) extract was determined with GC-MS.
31831916	3	0	gly	glycosylation	563:575	arg2	sites					577:581	the N-glycoproteins and glycosylation sites	539:581	the N-glycoproteins and glycosylation sites identified through their deglycosylated forms of glycosite-containing peptides	539:660	In this paper, we reviewed the human glycoproteomic profiles that have been reported in more than 80 individual studies, and mainly focused on the N-glycoproteins and glycosylation sites identified through their deglycosylated forms of glycosite-containing peptides.
31831916	1	1	theme	biological	167:176	arg1	research					178:185	clinical and biological research	154:185	clinical and biological research	154:185	N-linked glycoprotein is a highly interesting class of proteins for clinical and biological research.
31831916	5	2	theme	tissues	1025:1031	arg1	analysis					999:1006	a systematical glycoproteomic analysis	969:1006	a systematical glycoproteomic analysis of various human tissues and body fluids using a uniform platform	969:1072	As the glycoproteomic data is still missing for many organs and tissues, a systematical glycoproteomic analysis of various human tissues and body fluids using a uniform platform is still needed for an integrated map of human N-glycoproteomes.
31831916	2	3	theme	sites	276:280	arg1	profiling					222:230	large-scale profiling	210:230	large-scale profiling of N-linked glycoproteins and glycosylation sites from biological and clinical samples	210:317	Over the last decade, large-scale profiling of N-linked glycoproteins and glycosylation sites from biological and clinical samples has been achieved through mass spectrometry-based glycoproteomic approaches.
31831916	0	4	link	N-linked	14:21	arg1	glycoproteins					23:35	human N-linked glycoproteins	8:35	human N-linked glycoproteins	8:35	Mapping human N-linked glycoproteins and glycosylation sites using mass spectrometry.
31831916	3	5	gly	N-glycoproteins	543:557	arg1	N-glycoproteins					543:557	N-glycoproteins	543:557	N-glycoproteins	543:557	In this paper, we reviewed the human glycoproteomic profiles that have been reported in more than 80 individual studies, and mainly focused on the N-glycoproteins and glycosylation sites identified through their deglycosylated forms of glycosite-containing peptides.
31831916	5	6	theme	body	1037:1040	arg1	fluids					1042:1047	body fluids	1037:1047	body fluids	1037:1047	As the glycoproteomic data is still missing for many organs and tissues, a systematical glycoproteomic analysis of various human tissues and body fluids using a uniform platform is still needed for an integrated map of human N-glycoproteomes.
31831916	1	7	gly	glycoprotein	95:106	arg1	class					132:136	a highly interesting class	111:136	a highly interesting class of proteins for clinical and biological research	111:185	N-linked glycoprotein is a highly interesting class of proteins for clinical and biological research.
31831916	1	7	gly	glycoprotein	95:106	arg1	glycoprotein					95:106	N-linked glycoprotein	86:106	N-linked glycoprotein	86:106	N-linked glycoprotein is a highly interesting class of proteins for clinical and biological research.
31831916	5	8	theme	N-glycoproteomes	1121:1136	arg1	map					1108:1110	an integrated map	1094:1110	an integrated map of human N-glycoproteomes	1094:1136	As the glycoproteomic data is still missing for many organs and tissues, a systematical glycoproteomic analysis of various human tissues and body fluids using a uniform platform is still needed for an integrated map of human N-glycoproteomes.
31831916	4	9	theme	human	828:832	arg1	tissues					834:840	twelve human tissues	821:840	twelve human tissues	821:840	According to our analyses, more than 30,000 glycosite-containing peptides and 7,000 human glycoproteins have been identified from five different body fluids, twelve human tissues (or related cell lines), and four special cell types.
31831916	2	10	theme	clinical	302:309	arg1	samples					311:317	biological and clinical samples	287:317	biological and clinical samples	287:317	Over the last decade, large-scale profiling of N-linked glycoproteins and glycosylation sites from biological and clinical samples has been achieved through mass spectrometry-based glycoproteomic approaches.
31831916	1	11	theme	proteins	141:148	arg1	class					132:136	a highly interesting class	111:136	a highly interesting class of proteins for clinical and biological research	111:185	N-linked glycoprotein is a highly interesting class of proteins for clinical and biological research.
31831916	1	11	theme	proteins	141:148	arg1	glycoprotein					95:106	N-linked glycoprotein	86:106	N-linked glycoprotein	86:106	N-linked glycoprotein is a highly interesting class of proteins for clinical and biological research.
31831916	5	12	theme	glycoproteomic	903:916	arg1	data					918:921	the glycoproteomic data	899:921	the glycoproteomic data	899:921	As the glycoproteomic data is still missing for many organs and tissues, a systematical glycoproteomic analysis of various human tissues and body fluids using a uniform platform is still needed for an integrated map of human N-glycoproteomes.
31831916	5	13	theme	human	1019:1023	arg1	tissues					1025:1031	various human tissues	1011:1031	various human tissues	1011:1031	As the glycoproteomic data is still missing for many organs and tissues, a systematical glycoproteomic analysis of various human tissues and body fluids using a uniform platform is still needed for an integrated map of human N-glycoproteomes.
31831916	2	14	from	profiling	222:230	arg1	samples					311:317	biological and clinical samples	287:317	biological and clinical samples	287:317	Over the last decade, large-scale profiling of N-linked glycoproteins and glycosylation sites from biological and clinical samples has been achieved through mass spectrometry-based glycoproteomic approaches.
31831916	4	15	theme	cell	884:887	arg1	types					889:893	four special cell types	871:893	four special cell types	871:893	According to our analyses, more than 30,000 glycosite-containing peptides and 7,000 human glycoproteins have been identified from five different body fluids, twelve human tissues (or related cell lines), and four special cell types.
31831916	1	16	theme	N-linked	86:93	arg1	class					132:136	a highly interesting class	111:136	a highly interesting class of proteins for clinical and biological research	111:185	N-linked glycoprotein is a highly interesting class of proteins for clinical and biological research.
31831916	1	16	theme	N-linked	86:93	arg1	glycoprotein					95:106	N-linked glycoprotein	86:106	N-linked glycoprotein	86:106	N-linked glycoprotein is a highly interesting class of proteins for clinical and biological research.
31831916	4	17	theme	related	846:852	arg1	lines					859:863	related cell lines	846:863	related cell lines	846:863	According to our analyses, more than 30,000 glycosite-containing peptides and 7,000 human glycoproteins have been identified from five different body fluids, twelve human tissues (or related cell lines), and four special cell types.
31831916	0	18	theme	human	8:12	arg1	glycoproteins					23:35	human N-linked glycoproteins	8:35	human N-linked glycoproteins	8:35	Mapping human N-linked glycoproteins and glycosylation sites using mass spectrometry.
31831916	3	19	theme	N-glycoproteins	543:557	arg1	sites					577:581	the N-glycoproteins and glycosylation sites	539:581	the N-glycoproteins and glycosylation sites identified through their deglycosylated forms of glycosite-containing peptides	539:660	In this paper, we reviewed the human glycoproteomic profiles that have been reported in more than 80 individual studies, and mainly focused on the N-glycoproteins and glycosylation sites identified through their deglycosylated forms of glycosite-containing peptides.
31831916	1	20	link	N-linked	86:93	arg1	class					132:136	a highly interesting class	111:136	a highly interesting class of proteins for clinical and biological research	111:185	N-linked glycoprotein is a highly interesting class of proteins for clinical and biological research.
31831916	1	20	link	N-linked	86:93	arg1	glycoprotein					95:106	N-linked glycoprotein	86:106	N-linked glycoprotein	86:106	N-linked glycoprotein is a highly interesting class of proteins for clinical and biological research.
31831916	3	21	theme	deglycosylated	608:621	arg1	forms					623:627	their deglycosylated forms	602:627	their deglycosylated forms of glycosite-containing peptides	602:660	In this paper, we reviewed the human glycoproteomic profiles that have been reported in more than 80 individual studies, and mainly focused on the N-glycoproteins and glycosylation sites identified through their deglycosylated forms of glycosite-containing peptides.
31831916	4	22	theme	body	808:811	arg1	fluids					813:818	five different body fluids	793:818	five different body fluids	793:818	According to our analyses, more than 30,000 glycosite-containing peptides and 7,000 human glycoproteins have been identified from five different body fluids, twelve human tissues (or related cell lines), and four special cell types.
31831916	5	23	theme	fluids	1042:1047	arg1	analysis					999:1006	a systematical glycoproteomic analysis	969:1006	a systematical glycoproteomic analysis of various human tissues and body fluids using a uniform platform	969:1072	As the glycoproteomic data is still missing for many organs and tissues, a systematical glycoproteomic analysis of various human tissues and body fluids using a uniform platform is still needed for an integrated map of human N-glycoproteomes.
31831916	3	24	theme	glycosite-containing	632:651	arg1	peptides					653:660	glycosite-containing peptides	632:660	glycosite-containing peptides	632:660	In this paper, we reviewed the human glycoproteomic profiles that have been reported in more than 80 individual studies, and mainly focused on the N-glycoproteins and glycosylation sites identified through their deglycosylated forms of glycosite-containing peptides.
31831916	5	25	theme	glycoproteomic	984:997	arg1	analysis					999:1006	a systematical glycoproteomic analysis	969:1006	a systematical glycoproteomic analysis of various human tissues and body fluids using a uniform platform	969:1072	As the glycoproteomic data is still missing for many organs and tissues, a systematical glycoproteomic analysis of various human tissues and body fluids using a uniform platform is still needed for an integrated map of human N-glycoproteomes.
31831916	3	26	theme	peptides	653:660	arg1	forms					623:627	their deglycosylated forms	602:627	their deglycosylated forms of glycosite-containing peptides	602:660	In this paper, we reviewed the human glycoproteomic profiles that have been reported in more than 80 individual studies, and mainly focused on the N-glycoproteins and glycosylation sites identified through their deglycosylated forms of glycosite-containing peptides.
31831916	2	27	gly	glycosylation	262:274	arg2	sites					276:280	glycosylation sites	262:280	glycosylation sites	262:280	Over the last decade, large-scale profiling of N-linked glycoproteins and glycosylation sites from biological and clinical samples has been achieved through mass spectrometry-based glycoproteomic approaches.
31831916	5	28	theme	systematical	971:982	arg1	analysis					999:1006	a systematical glycoproteomic analysis	969:1006	a systematical glycoproteomic analysis of various human tissues and body fluids using a uniform platform	969:1072	As the glycoproteomic data is still missing for many organs and tissues, a systematical glycoproteomic analysis of various human tissues and body fluids using a uniform platform is still needed for an integrated map of human N-glycoproteomes.
31831916	5	29	theme	integrated	1097:1106	arg1	map					1108:1110	an integrated map	1094:1110	an integrated map of human N-glycoproteomes	1094:1136	As the glycoproteomic data is still missing for many organs and tissues, a systematical glycoproteomic analysis of various human tissues and body fluids using a uniform platform is still needed for an integrated map of human N-glycoproteomes.
31831916	5	30	theme	many	944:947	arg1	organs					949:954	many organs	944:954	many organs	944:954	As the glycoproteomic data is still missing for many organs and tissues, a systematical glycoproteomic analysis of various human tissues and body fluids using a uniform platform is still needed for an integrated map of human N-glycoproteomes.
31831916	3	31	theme	individual	497:506	arg1	studies					508:514	more than 80 individual studies	484:514	more than 80 individual studies	484:514	In this paper, we reviewed the human glycoproteomic profiles that have been reported in more than 80 individual studies, and mainly focused on the N-glycoproteins and glycosylation sites identified through their deglycosylated forms of glycosite-containing peptides.
31831916	0	32	theme	glycosylation	41:53	arg1	sites					55:59	glycosylation sites	41:59	glycosylation sites	41:59	Mapping human N-linked glycoproteins and glycosylation sites using mass spectrometry.
31831916	3	33	gly	deglycosylated	608:621	arg1	peptides					653:660	glycosite-containing peptides	632:660	glycosite-containing peptides	632:660	In this paper, we reviewed the human glycoproteomic profiles that have been reported in more than 80 individual studies, and mainly focused on the N-glycoproteins and glycosylation sites identified through their deglycosylated forms of glycosite-containing peptides.
31831916	3	33	gly	deglycosylated	608:621	arg1	forms					623:627	their deglycosylated forms	602:627	their deglycosylated forms of glycosite-containing peptides	602:660	In this paper, we reviewed the human glycoproteomic profiles that have been reported in more than 80 individual studies, and mainly focused on the N-glycoproteins and glycosylation sites identified through their deglycosylated forms of glycosite-containing peptides.
31831916	3	34	theme	glycosylation	563:575	arg1	sites					577:581	the N-glycoproteins and glycosylation sites	539:581	the N-glycoproteins and glycosylation sites identified through their deglycosylated forms of glycosite-containing peptides	539:660	In this paper, we reviewed the human glycoproteomic profiles that have been reported in more than 80 individual studies, and mainly focused on the N-glycoproteins and glycosylation sites identified through their deglycosylated forms of glycosite-containing peptides.
31831916	2	35	theme	glycoproteins	244:256	arg1	profiling					222:230	large-scale profiling	210:230	large-scale profiling of N-linked glycoproteins and glycosylation sites from biological and clinical samples	210:317	Over the last decade, large-scale profiling of N-linked glycoproteins and glycosylation sites from biological and clinical samples has been achieved through mass spectrometry-based glycoproteomic approaches.
31831916	2	36	gly	glycoproteins	244:256	arg1	glycoproteins					244:256	N-linked glycoproteins	235:256	N-linked glycoproteins	235:256	Over the last decade, large-scale profiling of N-linked glycoproteins and glycosylation sites from biological and clinical samples has been achieved through mass spectrometry-based glycoproteomic approaches.
31831916	2	37	theme	N-linked	235:242	arg1	glycoproteins					244:256	N-linked glycoproteins	235:256	N-linked glycoproteins	235:256	Over the last decade, large-scale profiling of N-linked glycoproteins and glycosylation sites from biological and clinical samples has been achieved through mass spectrometry-based glycoproteomic approaches.
31831916	3	38	theme	human	427:431	arg1	profiles					448:455	the human glycoproteomic profiles	423:455	the human glycoproteomic profiles that have been reported in more than 80 individual studies	423:514	In this paper, we reviewed the human glycoproteomic profiles that have been reported in more than 80 individual studies, and mainly focused on the N-glycoproteins and glycosylation sites identified through their deglycosylated forms of glycosite-containing peptides.
31831916	5	39	theme	human	1115:1119	arg1	N-glycoproteomes					1121:1136	human N-glycoproteomes	1115:1136	human N-glycoproteomes	1115:1136	As the glycoproteomic data is still missing for many organs and tissues, a systematical glycoproteomic analysis of various human tissues and body fluids using a uniform platform is still needed for an integrated map of human N-glycoproteomes.
31831916	4	40	theme	special	876:882	arg1	types					889:893	four special cell types	871:893	four special cell types	871:893	According to our analyses, more than 30,000 glycosite-containing peptides and 7,000 human glycoproteins have been identified from five different body fluids, twelve human tissues (or related cell lines), and four special cell types.
31831916	1	41	theme	interesting	120:130	arg1	class					132:136	a highly interesting class	111:136	a highly interesting class of proteins for clinical and biological research	111:185	N-linked glycoprotein is a highly interesting class of proteins for clinical and biological research.
31831916	1	41	theme	interesting	120:130	arg1	glycoprotein					95:106	N-linked glycoprotein	86:106	N-linked glycoprotein	86:106	N-linked glycoprotein is a highly interesting class of proteins for clinical and biological research.
31831916	3	42	theme	glycoproteomic	433:446	arg1	profiles					448:455	the human glycoproteomic profiles	423:455	the human glycoproteomic profiles that have been reported in more than 80 individual studies	423:514	In this paper, we reviewed the human glycoproteomic profiles that have been reported in more than 80 individual studies, and mainly focused on the N-glycoproteins and glycosylation sites identified through their deglycosylated forms of glycosite-containing peptides.
31831916	4	43	gly	glycoproteins	753:765	arg1	glycoproteins					753:765	7,000 human glycoproteins	741:765	7,000 human glycoproteins	741:765	According to our analyses, more than 30,000 glycosite-containing peptides and 7,000 human glycoproteins have been identified from five different body fluids, twelve human tissues (or related cell lines), and four special cell types.
31831916	2	44	theme	large-scale	210:220	arg1	profiling					222:230	large-scale profiling	210:230	large-scale profiling of N-linked glycoproteins and glycosylation sites from biological and clinical samples	210:317	Over the last decade, large-scale profiling of N-linked glycoproteins and glycosylation sites from biological and clinical samples has been achieved through mass spectrometry-based glycoproteomic approaches.
31831916	0	45	gly	glycoproteins	23:35	arg1	glycoproteins					23:35	human N-linked glycoproteins	8:35	human N-linked glycoproteins	8:35	Mapping human N-linked glycoproteins and glycosylation sites using mass spectrometry.
31831916	2	46	theme	glycoproteomic	369:382	arg1	approaches					384:393	mass spectrometry-based glycoproteomic approaches	345:393	mass spectrometry-based glycoproteomic approaches	345:393	Over the last decade, large-scale profiling of N-linked glycoproteins and glycosylation sites from biological and clinical samples has been achieved through mass spectrometry-based glycoproteomic approaches.
31831916	5	47	theme	uniform	1057:1063	arg1	platform					1065:1072	a uniform platform	1055:1072	a uniform platform	1055:1072	As the glycoproteomic data is still missing for many organs and tissues, a systematical glycoproteomic analysis of various human tissues and body fluids using a uniform platform is still needed for an integrated map of human N-glycoproteomes.
31831916	4	48	theme	cell	854:857	arg1	lines					859:863	related cell lines	846:863	related cell lines	846:863	According to our analyses, more than 30,000 glycosite-containing peptides and 7,000 human glycoproteins have been identified from five different body fluids, twelve human tissues (or related cell lines), and four special cell types.
31831916	4	49	theme	human	747:751	arg1	glycoproteins					753:765	7,000 human glycoproteins	741:765	7,000 human glycoproteins	741:765	According to our analyses, more than 30,000 glycosite-containing peptides and 7,000 human glycoproteins have been identified from five different body fluids, twelve human tissues (or related cell lines), and four special cell types.
31831916	2	50	theme	spectrometry-based	350:367	arg1	approaches					384:393	mass spectrometry-based glycoproteomic approaches	345:393	mass spectrometry-based glycoproteomic approaches	345:393	Over the last decade, large-scale profiling of N-linked glycoproteins and glycosylation sites from biological and clinical samples has been achieved through mass spectrometry-based glycoproteomic approaches.
31831916	2	51	theme	biological	287:296	arg1	samples					311:317	biological and clinical samples	287:317	biological and clinical samples	287:317	Over the last decade, large-scale profiling of N-linked glycoproteins and glycosylation sites from biological and clinical samples has been achieved through mass spectrometry-based glycoproteomic approaches.
31831916	0	52	gly	glycosylation	41:53	arg2	sites					55:59	glycosylation sites	41:59	glycosylation sites	41:59	Mapping human N-linked glycoproteins and glycosylation sites using mass spectrometry.
31831916	2	53	theme	mass	345:348	arg1	approaches					384:393	mass spectrometry-based glycoproteomic approaches	345:393	mass spectrometry-based glycoproteomic approaches	345:393	Over the last decade, large-scale profiling of N-linked glycoproteins and glycosylation sites from biological and clinical samples has been achieved through mass spectrometry-based glycoproteomic approaches.
31831916	2	54	from	samples	311:317	arg1	profiling					222:230	large-scale profiling	210:230	large-scale profiling of N-linked glycoproteins and glycosylation sites from biological and clinical samples	210:317	Over the last decade, large-scale profiling of N-linked glycoproteins and glycosylation sites from biological and clinical samples has been achieved through mass spectrometry-based glycoproteomic approaches.
31831916	2	54	from	samples	311:317	arg1	glycoproteins					244:256	N-linked glycoproteins	235:256	N-linked glycoproteins	235:256	Over the last decade, large-scale profiling of N-linked glycoproteins and glycosylation sites from biological and clinical samples has been achieved through mass spectrometry-based glycoproteomic approaches.
31831916	2	54	from	samples	311:317	arg1	sites					276:280	glycosylation sites	262:280	glycosylation sites	262:280	Over the last decade, large-scale profiling of N-linked glycoproteins and glycosylation sites from biological and clinical samples has been achieved through mass spectrometry-based glycoproteomic approaches.
31831916	4	55	theme	different	798:806	arg1	fluids					813:818	five different body fluids	793:818	five different body fluids	793:818	According to our analyses, more than 30,000 glycosite-containing peptides and 7,000 human glycoproteins have been identified from five different body fluids, twelve human tissues (or related cell lines), and four special cell types.
31831916	2	56	theme	last	197:200	arg1	decade					202:207	the last decade	193:207	the last decade	193:207	Over the last decade, large-scale profiling of N-linked glycoproteins and glycosylation sites from biological and clinical samples has been achieved through mass spectrometry-based glycoproteomic approaches.
31831916	2	57	theme	glycosylation	262:274	arg1	sites					276:280	glycosylation sites	262:280	glycosylation sites	262:280	Over the last decade, large-scale profiling of N-linked glycoproteins and glycosylation sites from biological and clinical samples has been achieved through mass spectrometry-based glycoproteomic approaches.
31831916	4	58	theme	glycosite-containing	707:726	arg1	peptides					728:735	more than 30,000 glycosite-containing peptides	690:735	more than 30,000 glycosite-containing peptides	690:735	According to our analyses, more than 30,000 glycosite-containing peptides and 7,000 human glycoproteins have been identified from five different body fluids, twelve human tissues (or related cell lines), and four special cell types.
31831916	0	59	theme	mass	67:70	arg1	spectrometry					72:83	mass spectrometry	67:83	mass spectrometry	67:83	Mapping human N-linked glycoproteins and glycosylation sites using mass spectrometry.
31831916	5	60	theme	various	1011:1017	arg1	tissues					1025:1031	various human tissues	1011:1031	various human tissues	1011:1031	As the glycoproteomic data is still missing for many organs and tissues, a systematical glycoproteomic analysis of various human tissues and body fluids using a uniform platform is still needed for an integrated map of human N-glycoproteomes.
31831916	0	61	theme	N-linked	14:21	arg1	glycoproteins					23:35	human N-linked glycoproteins	8:35	human N-linked glycoproteins	8:35	Mapping human N-linked glycoproteins and glycosylation sites using mass spectrometry.
31831916	1	62	theme	clinical	154:161	arg1	research					178:185	clinical and biological research	154:185	clinical and biological research	154:185	N-linked glycoprotein is a highly interesting class of proteins for clinical and biological research.
31831916	2	63	link	N-linked	235:242	arg1	glycoproteins					244:256	N-linked glycoproteins	235:256	N-linked glycoproteins	235:256	Over the last decade, large-scale profiling of N-linked glycoproteins and glycosylation sites from biological and clinical samples has been achieved through mass spectrometry-based glycoproteomic approaches.
30110170	7	0	theme	glycoforms	1161:1170	arg1	levels					1139:1144	increased levels	1129:1144	increased levels of fucosylated glycoforms	1129:1170	In addition, increased levels of fucosylated glycoforms were associated with a reduction in survival time.
30110170	4	1	theme	no	825:826	arg1	cancer					828:833	no cancer	825:833	no cancer	825:833	We used this methodology in the analysis of 138 HCC tissue samples and compared the N-linked glycans in cancer tissue with either adjacent untransformed or tissue from patients with liver cirrhosis but no cancer.
30110170	6	2	theme	increased	1044:1052	arg1	levels					1054:1059	increased levels	1044:1059	increased levels of branching with or without any fucose modifications	1044:1113	These glycans fell into two major classes, those with increased levels of fucosylation and those with increased levels of branching with or without any fucose modifications.
30110170	8	3	from	levels	1276:1281	arg1	serum					1322:1326	HCC serum	1318:1326	HCC serum	1318:1326	This work supports the hypothesis that the increased levels of fucosylated N-linked glycans in HCC serum are produced directly from the cancer tissue.
30110170	7	4	from	reduction	1195:1203	arg1	time					1217:1220	survival time	1208:1220	survival time	1208:1220	In addition, increased levels of fucosylated glycoforms were associated with a reduction in survival time.
30110170	3	5	theme	tissue	495:500	arg1	imaging					524:530	in situ tissue based N-linked glycan imaging	487:530	in situ tissue based N-linked glycan imaging	487:530	Our laboratory previously developed a method of in situ tissue based N-linked glycan imaging that bypasses the need for microdissection and solubilization of tissue prior to analysis.
30110170	4	6	with	patients	791:798	arg1	cirrhosis					811:819	liver cirrhosis	805:819	liver cirrhosis	805:819	We used this methodology in the analysis of 138 HCC tissue samples and compared the N-linked glycans in cancer tissue with either adjacent untransformed or tissue from patients with liver cirrhosis but no cancer.
30110170	4	6	with	patients	791:798	arg1	cancer					828:833	no cancer	825:833	no cancer	825:833	We used this methodology in the analysis of 138 HCC tissue samples and compared the N-linked glycans in cancer tissue with either adjacent untransformed or tissue from patients with liver cirrhosis but no cancer.
30110170	7	7	theme	fucosylated	1149:1159	arg1	glycoforms					1161:1170	fucosylated glycoforms	1149:1170	fucosylated glycoforms	1149:1170	In addition, increased levels of fucosylated glycoforms were associated with a reduction in survival time.
30110170	6	8	theme	fucose	1094:1099	arg1	modifications					1101:1113	any fucose modifications	1090:1113	any fucose modifications	1090:1113	These glycans fell into two major classes, those with increased levels of fucosylation and those with increased levels of branching with or without any fucose modifications.
30110170	0	9	dep	in	102:103	arg1	Situ					105:108	Situ	105:108	Situ	105:108	N-Linked Glycan Branching and Fucosylation Are Increased Directly in Hcc Tissue As Determined through in Situ Glycan Imaging.
30110170	4	10	from	glycans	716:722	arg1	tissue					734:739	cancer tissue	727:739	cancer tissue	727:739	We used this methodology in the analysis of 138 HCC tissue samples and compared the N-linked glycans in cancer tissue with either adjacent untransformed or tissue from patients with liver cirrhosis but no cancer.
30110170	3	11	theme	based	502:506	arg1	imaging					524:530	in situ tissue based N-linked glycan imaging	487:530	in situ tissue based N-linked glycan imaging	487:530	Our laboratory previously developed a method of in situ tissue based N-linked glycan imaging that bypasses the need for microdissection and solubilization of tissue prior to analysis.
30110170	4	12	link	N-linked	707:714	arg1	glycans					716:722	the N-linked glycans	703:722	the N-linked glycans in cancer tissue	703:739	We used this methodology in the analysis of 138 HCC tissue samples and compared the N-linked glycans in cancer tissue with either adjacent untransformed or tissue from patients with liver cirrhosis but no cancer.
30110170	7	13	theme	increased	1129:1137	arg1	levels					1139:1144	increased levels	1129:1144	increased levels of fucosylated glycoforms	1129:1170	In addition, increased levels of fucosylated glycoforms were associated with a reduction in survival time.
30110170	3	14	theme	in	487:488	arg1	imaging					524:530	in situ tissue based N-linked glycan imaging	487:530	in situ tissue based N-linked glycan imaging	487:530	Our laboratory previously developed a method of in situ tissue based N-linked glycan imaging that bypasses the need for microdissection and solubilization of tissue prior to analysis.
30110170	0	15	theme	in	102:103	arg1	Imaging					117:123	in Situ Glycan Imaging	102:123	in Situ Glycan Imaging	102:123	N-Linked Glycan Branching and Fucosylation Are Increased Directly in Hcc Tissue As Determined through in Situ Glycan Imaging.
30110170	8	16	theme	HCC	1318:1320	arg1	serum					1322:1326	HCC serum	1318:1326	HCC serum	1318:1326	This work supports the hypothesis that the increased levels of fucosylated N-linked glycans in HCC serum are produced directly from the cancer tissue.
30110170	6	17	theme	branching	1064:1072	arg1	levels					1054:1059	increased levels	1044:1059	increased levels of branching with or without any fucose modifications	1044:1113	These glycans fell into two major classes, those with increased levels of fucosylation and those with increased levels of branching with or without any fucose modifications.
30110170	6	18	theme	major	970:974	arg1	those					1033:1037	those	1033:1037	those	1033:1037	These glycans fell into two major classes, those with increased levels of fucosylation and those with increased levels of branching with or without any fucose modifications.
30110170	6	18	theme	major	970:974	arg1	those					985:989	those	985:989	those	985:989	These glycans fell into two major classes, those with increased levels of fucosylation and those with increased levels of branching with or without any fucose modifications.
30110170	6	18	theme	major	970:974	arg1	classes					976:982	two major classes	966:982	two major classes	966:982	These glycans fell into two major classes, those with increased levels of fucosylation and those with increased levels of branching with or without any fucose modifications.
30110170	8	19	theme	increased	1266:1274	arg1	levels					1276:1281	the increased levels	1262:1281	the increased levels of fucosylated N-linked glycans in HCC serum	1262:1326	This work supports the hypothesis that the increased levels of fucosylated N-linked glycans in HCC serum are produced directly from the cancer tissue.
30110170	6	20	theme	fucosylation	1016:1027	arg1	levels					1006:1011	increased levels	996:1011	increased levels of fucosylation	996:1027	These glycans fell into two major classes, those with increased levels of fucosylation and those with increased levels of branching with or without any fucose modifications.
30110170	0	21	theme	Glycan	9:14	arg1	Branching					16:24	N-Linked Glycan Branching	0:24	N-Linked Glycan Branching	0:24	N-Linked Glycan Branching and Fucosylation Are Increased Directly in Hcc Tissue As Determined through in Situ Glycan Imaging.
30110170	4	22	from	patients	791:798	arg1	tissue					779:784	either adjacent untransformed or tissue	746:784	either adjacent untransformed or tissue from patients with liver cirrhosis but no cancer	746:833	We used this methodology in the analysis of 138 HCC tissue samples and compared the N-linked glycans in cancer tissue with either adjacent untransformed or tissue from patients with liver cirrhosis but no cancer.
30110170	3	23	link	N-linked	508:515	arg1	imaging					524:530	in situ tissue based N-linked glycan imaging	487:530	in situ tissue based N-linked glycan imaging	487:530	Our laboratory previously developed a method of in situ tissue based N-linked glycan imaging that bypasses the need for microdissection and solubilization of tissue prior to analysis.
30110170	3	24	theme	imaging	524:530	arg1	method					477:482	a method	475:482	a method of in situ tissue based N-linked glycan imaging that bypasses the need for microdissection and solubilization of tissue prior to analysis	475:620	Our laboratory previously developed a method of in situ tissue based N-linked glycan imaging that bypasses the need for microdissection and solubilization of tissue prior to analysis.
30110170	0	25	theme	N-Linked	0:7	arg1	Branching					16:24	N-Linked Glycan Branching	0:24	N-Linked Glycan Branching	0:24	N-Linked Glycan Branching and Fucosylation Are Increased Directly in Hcc Tissue As Determined through in Situ Glycan Imaging.
30110170	1	26	theme	deaths	245:250	arg1	annually					252:259	more than 700,000 deaths annually	227:259	more than 700,000 deaths annually	227:259	Hepatocellular carcinoma (HCC) remains as the fifth most common cancer in the world and accounts for more than 700,000 deaths annually.
30110170	4	27	theme	liver	805:809	arg1	cirrhosis					811:819	liver cirrhosis	805:819	liver cirrhosis	805:819	We used this methodology in the analysis of 138 HCC tissue samples and compared the N-linked glycans in cancer tissue with either adjacent untransformed or tissue from patients with liver cirrhosis but no cancer.
30110170	4	28	theme	samples	682:688	arg1	analysis					655:662	the analysis	651:662	the analysis of 138 HCC tissue samples	651:688	We used this methodology in the analysis of 138 HCC tissue samples and compared the N-linked glycans in cancer tissue with either adjacent untransformed or tissue from patients with liver cirrhosis but no cancer.
30110170	1	29	theme	fifth	172:176	arg1	cancer					190:195	the fifth most common cancer	168:195	the fifth most common cancer in the world	168:208	Hepatocellular carcinoma (HCC) remains as the fifth most common cancer in the world and accounts for more than 700,000 deaths annually.
30110170	5	30	theme	HCC	885:887	arg1	tissues					889:895	HCC tissues	885:895	HCC tissues	885:895	Ten glycans were found significantly elevated in HCC tissues as compared to cirrhotic or adjacent tissue.
30110170	2	31	theme	serum	273:277	arg1	glycosylation					279:291	serum glycosylation	273:291	serum glycosylation	273:291	Changes in serum glycosylation have long been associated with this cancer but the source of that material is unknown and direct glycan analysis of HCC tissues has been limited.
30110170	2	32	theme	direct	383:388	arg1	analysis					397:404	direct glycan analysis	383:404	direct glycan analysis of HCC tissues	383:419	Changes in serum glycosylation have long been associated with this cancer but the source of that material is unknown and direct glycan analysis of HCC tissues has been limited.
30110170	2	33	theme	glycan	390:395	arg1	analysis					397:404	direct glycan analysis	383:404	direct glycan analysis of HCC tissues	383:419	Changes in serum glycosylation have long been associated with this cancer but the source of that material is unknown and direct glycan analysis of HCC tissues has been limited.
30110170	8	34	link	N-linked	1298:1305	arg1	glycans					1307:1313	fucosylated N-linked glycans	1286:1313	fucosylated N-linked glycans	1286:1313	This work supports the hypothesis that the increased levels of fucosylated N-linked glycans in HCC serum are produced directly from the cancer tissue.
30110170	0	35	theme	Glycan	110:115	arg1	Imaging					117:123	in Situ Glycan Imaging	102:123	in Situ Glycan Imaging	102:123	N-Linked Glycan Branching and Fucosylation Are Increased Directly in Hcc Tissue As Determined through in Situ Glycan Imaging.
30110170	4	36	theme	N-linked	707:714	arg1	glycans					716:722	the N-linked glycans	703:722	the N-linked glycans in cancer tissue	703:739	We used this methodology in the analysis of 138 HCC tissue samples and compared the N-linked glycans in cancer tissue with either adjacent untransformed or tissue from patients with liver cirrhosis but no cancer.
30110170	3	37	theme	glycan	517:522	arg1	imaging					524:530	in situ tissue based N-linked glycan imaging	487:530	in situ tissue based N-linked glycan imaging	487:530	Our laboratory previously developed a method of in situ tissue based N-linked glycan imaging that bypasses the need for microdissection and solubilization of tissue prior to analysis.
30110170	1	38	theme	common	183:188	arg1	cancer					190:195	the fifth most common cancer	168:195	the fifth most common cancer in the world	168:208	Hepatocellular carcinoma (HCC) remains as the fifth most common cancer in the world and accounts for more than 700,000 deaths annually.
30110170	8	39	theme	glycans	1307:1313	arg1	levels					1276:1281	the increased levels	1262:1281	the increased levels of fucosylated N-linked glycans in HCC serum	1262:1326	This work supports the hypothesis that the increased levels of fucosylated N-linked glycans in HCC serum are produced directly from the cancer tissue.
30110170	8	40	gly	fucosylated	1286:1296	arg1	glycans					1307:1313	fucosylated N-linked glycans	1286:1313	fucosylated N-linked glycans	1286:1313	This work supports the hypothesis that the increased levels of fucosylated N-linked glycans in HCC serum are produced directly from the cancer tissue.
30110170	4	41	theme	or	776:777	arg1	tissue					779:784	either adjacent untransformed or tissue	746:784	either adjacent untransformed or tissue from patients with liver cirrhosis but no cancer	746:833	We used this methodology in the analysis of 138 HCC tissue samples and compared the N-linked glycans in cancer tissue with either adjacent untransformed or tissue from patients with liver cirrhosis but no cancer.
30110170	8	42	theme	fucosylated	1286:1296	arg1	glycans					1307:1313	fucosylated N-linked glycans	1286:1313	fucosylated N-linked glycans	1286:1313	This work supports the hypothesis that the increased levels of fucosylated N-linked glycans in HCC serum are produced directly from the cancer tissue.
30110170	3	43	theme	tissue	597:602	arg1	solubilization					579:592	solubilization	579:592	solubilization	579:592	Our laboratory previously developed a method of in situ tissue based N-linked glycan imaging that bypasses the need for microdissection and solubilization of tissue prior to analysis.
30110170	3	43	theme	tissue	597:602	arg1	microdissection					559:573	microdissection	559:573	microdissection	559:573	Our laboratory previously developed a method of in situ tissue based N-linked glycan imaging that bypasses the need for microdissection and solubilization of tissue prior to analysis.
30110170	4	44	theme	untransformed	762:774	arg1	tissue					779:784	either adjacent untransformed or tissue	746:784	either adjacent untransformed or tissue from patients with liver cirrhosis but no cancer	746:833	We used this methodology in the analysis of 138 HCC tissue samples and compared the N-linked glycans in cancer tissue with either adjacent untransformed or tissue from patients with liver cirrhosis but no cancer.
30110170	1	45	from	cancer	190:195	arg1	world					204:208	the world	200:208	the world	200:208	Hepatocellular carcinoma (HCC) remains as the fifth most common cancer in the world and accounts for more than 700,000 deaths annually.
30110170	8	46	theme	N-linked	1298:1305	arg1	glycans					1307:1313	fucosylated N-linked glycans	1286:1313	fucosylated N-linked glycans	1286:1313	This work supports the hypothesis that the increased levels of fucosylated N-linked glycans in HCC serum are produced directly from the cancer tissue.
30110170	3	47	theme	prior	604:608	arg1	tissue					597:602	tissue	597:602	tissue prior to analysis	597:620	Our laboratory previously developed a method of in situ tissue based N-linked glycan imaging that bypasses the need for microdissection and solubilization of tissue prior to analysis.
30110170	8	48	theme	cancer	1359:1364	arg1	tissue					1366:1371	the cancer tissue	1355:1371	the cancer tissue	1355:1371	This work supports the hypothesis that the increased levels of fucosylated N-linked glycans in HCC serum are produced directly from the cancer tissue.
30110170	5	49	dep	found	853:857	arg1	elevated					873:880	elevated	873:880	were found significantly elevated in HCC tissues as compared to cirrhotic or adjacent tissue	848:939	Ten glycans were found significantly elevated in HCC tissues as compared to cirrhotic or adjacent tissue.
30110170	3	50	dep	in	487:488	arg1	situ					490:493	situ	490:493	situ	490:493	Our laboratory previously developed a method of in situ tissue based N-linked glycan imaging that bypasses the need for microdissection and solubilization of tissue prior to analysis.
30110170	2	51	theme	tissues	413:419	arg1	source					344:349	the source	340:349	the source of that material is unknown	340:377	Changes in serum glycosylation have long been associated with this cancer but the source of that material is unknown and direct glycan analysis of HCC tissues has been limited.
30110170	2	51	theme	tissues	413:419	arg1	analysis					397:404	direct glycan analysis	383:404	direct glycan analysis of HCC tissues	383:419	Changes in serum glycosylation have long been associated with this cancer but the source of that material is unknown and direct glycan analysis of HCC tissues has been limited.
30110170	7	52	theme	survival	1208:1215	arg1	time					1217:1220	survival time	1208:1220	survival time	1208:1220	In addition, increased levels of fucosylated glycoforms were associated with a reduction in survival time.
30110170	0	53	gly	Fucosylation	30:41	arg1	Tissue					73:78	Hcc Tissue	69:78	Hcc Tissue	69:78	N-Linked Glycan Branching and Fucosylation Are Increased Directly in Hcc Tissue As Determined through in Situ Glycan Imaging.
30110170	4	54	theme	tissue	675:680	arg1	samples					682:688	138 HCC tissue samples	667:688	138 HCC tissue samples	667:688	We used this methodology in the analysis of 138 HCC tissue samples and compared the N-linked glycans in cancer tissue with either adjacent untransformed or tissue from patients with liver cirrhosis but no cancer.
30110170	3	55	theme	N-linked	508:515	arg1	imaging					524:530	in situ tissue based N-linked glycan imaging	487:530	in situ tissue based N-linked glycan imaging	487:530	Our laboratory previously developed a method of in situ tissue based N-linked glycan imaging that bypasses the need for microdissection and solubilization of tissue prior to analysis.
30110170	4	56	theme	cancer	727:732	arg1	tissue					734:739	cancer tissue	727:739	cancer tissue	727:739	We used this methodology in the analysis of 138 HCC tissue samples and compared the N-linked glycans in cancer tissue with either adjacent untransformed or tissue from patients with liver cirrhosis but no cancer.
30110170	4	57	used	used	626:629	arg2	We					623:624	We	623:624	We	623:624	We used this methodology in the analysis of 138 HCC tissue samples and compared the N-linked glycans in cancer tissue with either adjacent untransformed or tissue from patients with liver cirrhosis but no cancer.
30110170	1	58	theme	Hepatocellular	126:139	arg1	HCC					152:154	HCC	152:154	HCC	152:154	Hepatocellular carcinoma (HCC) remains as the fifth most common cancer in the world and accounts for more than 700,000 deaths annually.
30110170	1	58	theme	Hepatocellular	126:139	arg1	carcinoma					141:149	Hepatocellular carcinoma	126:149	Hepatocellular carcinoma (HCC)	126:155	Hepatocellular carcinoma (HCC) remains as the fifth most common cancer in the world and accounts for more than 700,000 deaths annually.
30110170	2	59	from	Changes	262:268	arg1	glycosylation					279:291	serum glycosylation	273:291	serum glycosylation	273:291	Changes in serum glycosylation have long been associated with this cancer but the source of that material is unknown and direct glycan analysis of HCC tissues has been limited.
30110170	4	60	theme	HCC	671:673	arg1	samples					682:688	138 HCC tissue samples	667:688	138 HCC tissue samples	667:688	We used this methodology in the analysis of 138 HCC tissue samples and compared the N-linked glycans in cancer tissue with either adjacent untransformed or tissue from patients with liver cirrhosis but no cancer.
30110170	0	61	theme	Hcc	69:71	arg1	Tissue					73:78	Hcc Tissue	69:78	Hcc Tissue	69:78	N-Linked Glycan Branching and Fucosylation Are Increased Directly in Hcc Tissue As Determined through in Situ Glycan Imaging.
30110170	5	62	theme	adjacent	925:932	arg1	tissue					934:939	adjacent tissue	925:939	adjacent tissue	925:939	Ten glycans were found significantly elevated in HCC tissues as compared to cirrhotic or adjacent tissue.
30110170	4	63	theme	adjacent	753:760	arg1	tissue					779:784	either adjacent untransformed or tissue	746:784	either adjacent untransformed or tissue from patients with liver cirrhosis but no cancer	746:833	We used this methodology in the analysis of 138 HCC tissue samples and compared the N-linked glycans in cancer tissue with either adjacent untransformed or tissue from patients with liver cirrhosis but no cancer.
30110170	6	64	theme	increased	996:1004	arg1	levels					1006:1011	increased levels	996:1011	increased levels of fucosylation	996:1027	These glycans fell into two major classes, those with increased levels of fucosylation and those with increased levels of branching with or without any fucose modifications.
30110170	7	65	gly	fucosylated	1149:1159	arg1	glycoforms					1161:1170	fucosylated glycoforms	1149:1170	fucosylated glycoforms	1149:1170	In addition, increased levels of fucosylated glycoforms were associated with a reduction in survival time.
30110170	2	66	theme	HCC	409:411	arg1	tissues					413:419	HCC tissues	409:419	HCC tissues	409:419	Changes in serum glycosylation have long been associated with this cancer but the source of that material is unknown and direct glycan analysis of HCC tissues has been limited.
30225063	10	0	theme	estimated	1561:1569	arg1	quantities					1571:1580	the maximum estimated quantities	1549:1580	the maximum estimated quantities of Ca and Sr	1549:1593	Moreover, the maximum estimated quantities of Ca and Sr were found in S8.
30225063	3	1	theme	total	467:471	arg1	saponins					473:480	total saponins	467:480	total saponins	467:480	To better understand the nutritional value of A. elata, we compared total flavonoids, total saponins, phenolic compounds and mineral element contents in the buds of A. elata collected from eight different geographical regions (S1: Benxi; S2: Linjiang; S3: Pingwu; S4: Enshi; S5: Changbaishan; S6: Shangzhi; S7: Xiaoxinganling and S8: Harbin) in China.
30225063	12	2	from	evaluation	1798:1807	arg1	field					1849:1853	the food production field	1829:1853	the food production field	1829:1853	Our results provided an essential theoretical basis of quality evaluation of A. elata buds in the food production field.
30225063	3	3	from	regions	599:605	arg1	China					726:730	China	726:730	China	726:730	To better understand the nutritional value of A. elata, we compared total flavonoids, total saponins, phenolic compounds and mineral element contents in the buds of A. elata collected from eight different geographical regions (S1: Benxi; S2: Linjiang; S3: Pingwu; S4: Enshi; S5: Changbaishan; S6: Shangzhi; S7: Xiaoxinganling and S8: Harbin) in China.
30225063	3	4	theme	mineral	506:512	arg1	contents					522:529	mineral element contents	506:529	mineral element contents	506:529	To better understand the nutritional value of A. elata, we compared total flavonoids, total saponins, phenolic compounds and mineral element contents in the buds of A. elata collected from eight different geographical regions (S1: Benxi; S2: Linjiang; S3: Pingwu; S4: Enshi; S5: Changbaishan; S6: Shangzhi; S7: Xiaoxinganling and S8: Harbin) in China.
30225063	3	5	dep	S2	619:620	arg1	Pingwu					637:642	Pingwu	637:642	Pingwu	637:642	To better understand the nutritional value of A. elata, we compared total flavonoids, total saponins, phenolic compounds and mineral element contents in the buds of A. elata collected from eight different geographical regions (S1: Benxi; S2: Linjiang; S3: Pingwu; S4: Enshi; S5: Changbaishan; S6: Shangzhi; S7: Xiaoxinganling and S8: Harbin) in China.
30225063	3	5	dep	S2	619:620	arg1	S7					688:689	S7	688:689	S7	688:689	To better understand the nutritional value of A. elata, we compared total flavonoids, total saponins, phenolic compounds and mineral element contents in the buds of A. elata collected from eight different geographical regions (S1: Benxi; S2: Linjiang; S3: Pingwu; S4: Enshi; S5: Changbaishan; S6: Shangzhi; S7: Xiaoxinganling and S8: Harbin) in China.
30225063	3	5	dep	S2	619:620	arg1	Harbin					715:720	Harbin	715:720	S2: Linjiang; S3: Pingwu; S4: Enshi; S5: Changbaishan; S6: Shangzhi; S7: Xiaoxinganling and S8: Harbin	619:720	To better understand the nutritional value of A. elata, we compared total flavonoids, total saponins, phenolic compounds and mineral element contents in the buds of A. elata collected from eight different geographical regions (S1: Benxi; S2: Linjiang; S3: Pingwu; S4: Enshi; S5: Changbaishan; S6: Shangzhi; S7: Xiaoxinganling and S8: Harbin) in China.
30225063	3	5	dep	S2	619:620	arg1	S4					645:646	S4	645:646	S4	645:646	To better understand the nutritional value of A. elata, we compared total flavonoids, total saponins, phenolic compounds and mineral element contents in the buds of A. elata collected from eight different geographical regions (S1: Benxi; S2: Linjiang; S3: Pingwu; S4: Enshi; S5: Changbaishan; S6: Shangzhi; S7: Xiaoxinganling and S8: Harbin) in China.
30225063	3	5	dep	S2	619:620	arg1	Changbaishan					660:671	Changbaishan	660:671	Changbaishan	660:671	To better understand the nutritional value of A. elata, we compared total flavonoids, total saponins, phenolic compounds and mineral element contents in the buds of A. elata collected from eight different geographical regions (S1: Benxi; S2: Linjiang; S3: Pingwu; S4: Enshi; S5: Changbaishan; S6: Shangzhi; S7: Xiaoxinganling and S8: Harbin) in China.
30225063	3	5	dep	S2	619:620	arg1	Linjiang					623:630	Linjiang	623:630	Linjiang	623:630	To better understand the nutritional value of A. elata, we compared total flavonoids, total saponins, phenolic compounds and mineral element contents in the buds of A. elata collected from eight different geographical regions (S1: Benxi; S2: Linjiang; S3: Pingwu; S4: Enshi; S5: Changbaishan; S6: Shangzhi; S7: Xiaoxinganling and S8: Harbin) in China.
30225063	3	5	dep	S2	619:620	arg1	S6					674:675	S6	674:675	S2: Linjiang; S3: Pingwu; S4: Enshi; S5: Changbaishan; S6: Shangzhi; S7: Xiaoxinganling and S8: Harbin	619:720	To better understand the nutritional value of A. elata, we compared total flavonoids, total saponins, phenolic compounds and mineral element contents in the buds of A. elata collected from eight different geographical regions (S1: Benxi; S2: Linjiang; S3: Pingwu; S4: Enshi; S5: Changbaishan; S6: Shangzhi; S7: Xiaoxinganling and S8: Harbin) in China.
30225063	3	5	dep	S2	619:620	arg1	Xiaoxinganling					692:705	Xiaoxinganling	692:705	Xiaoxinganling	692:705	To better understand the nutritional value of A. elata, we compared total flavonoids, total saponins, phenolic compounds and mineral element contents in the buds of A. elata collected from eight different geographical regions (S1: Benxi; S2: Linjiang; S3: Pingwu; S4: Enshi; S5: Changbaishan; S6: Shangzhi; S7: Xiaoxinganling and S8: Harbin) in China.
30225063	3	5	dep	S2	619:620	arg1	S3					633:634	S3	633:634	S2: Linjiang; S3: Pingwu; S4: Enshi; S5: Changbaishan; S6: Shangzhi; S7: Xiaoxinganling and S8: Harbin	619:720	To better understand the nutritional value of A. elata, we compared total flavonoids, total saponins, phenolic compounds and mineral element contents in the buds of A. elata collected from eight different geographical regions (S1: Benxi; S2: Linjiang; S3: Pingwu; S4: Enshi; S5: Changbaishan; S6: Shangzhi; S7: Xiaoxinganling and S8: Harbin) in China.
30225063	3	5	dep	S2	619:620	arg1	Enshi					649:653	Enshi	649:653	Enshi	649:653	To better understand the nutritional value of A. elata, we compared total flavonoids, total saponins, phenolic compounds and mineral element contents in the buds of A. elata collected from eight different geographical regions (S1: Benxi; S2: Linjiang; S3: Pingwu; S4: Enshi; S5: Changbaishan; S6: Shangzhi; S7: Xiaoxinganling and S8: Harbin) in China.
30225063	3	5	dep	S2	619:620	arg1	S8					711:712	S8	711:712	S8	711:712	To better understand the nutritional value of A. elata, we compared total flavonoids, total saponins, phenolic compounds and mineral element contents in the buds of A. elata collected from eight different geographical regions (S1: Benxi; S2: Linjiang; S3: Pingwu; S4: Enshi; S5: Changbaishan; S6: Shangzhi; S7: Xiaoxinganling and S8: Harbin) in China.
30225063	3	5	dep	S2	619:620	arg1	S5					656:657	S5	656:657	S5	656:657	To better understand the nutritional value of A. elata, we compared total flavonoids, total saponins, phenolic compounds and mineral element contents in the buds of A. elata collected from eight different geographical regions (S1: Benxi; S2: Linjiang; S3: Pingwu; S4: Enshi; S5: Changbaishan; S6: Shangzhi; S7: Xiaoxinganling and S8: Harbin) in China.
30225063	3	5	dep	S2	619:620	arg1	Shangzhi					678:685	Shangzhi	678:685	Shangzhi	678:685	To better understand the nutritional value of A. elata, we compared total flavonoids, total saponins, phenolic compounds and mineral element contents in the buds of A. elata collected from eight different geographical regions (S1: Benxi; S2: Linjiang; S3: Pingwu; S4: Enshi; S5: Changbaishan; S6: Shangzhi; S7: Xiaoxinganling and S8: Harbin) in China.
30225063	12	6	theme	theoretical	1769:1779	arg1	basis					1781:1785	an essential theoretical basis	1756:1785	an essential theoretical basis of quality evaluation of A. elata buds in the food production field	1756:1853	Our results provided an essential theoretical basis of quality evaluation of A. elata buds in the food production field.
30225063	11	7	theme	chemical	1643:1650	arg1	composition					1652:1662	the chemical composition	1639:1662	the chemical composition in the buds of A. elata	1639:1686	This study indicated that the chemical composition in the buds of A. elata was obviously affected by geographical origin.
30225063	2	8	theme	species	279:285	arg1	bark					266:269	the root bark	257:269	the root bark of this species	257:285	Previous studies mainly focused on triterpene saponins in the root bark of this species, but little information existed concerning other chemical components, especially in the buds.
30225063	6	9	theme	predominant	1263:1273	arg1	compound					1284:1291	the predominant phenolic compound	1259:1291	the predominant phenolic compound	1259:1291	The total phenolic content in Enshi (S4) was significantly higher than others, and quercetin was the predominant phenolic compound in this sample.
30225063	6	9	theme	predominant	1263:1273	arg1	quercetin					1245:1253	quercetin	1245:1253	quercetin	1245:1253	The total phenolic content in Enshi (S4) was significantly higher than others, and quercetin was the predominant phenolic compound in this sample.
30225063	11	10	theme	elata	1682:1686	arg1	buds					1671:1674	the buds	1667:1674	the buds of A. elata	1667:1686	This study indicated that the chemical composition in the buds of A. elata was obviously affected by geographical origin.
30225063	4	11	theme	total	908:912	arg1	content					924:930	total flavonoid content	908:930	total flavonoid content (TFC, 4.06-48.63 mg g-1)	908:955	The results showed that the basic composition in the buds presented a wide variation, with ash (8.76-10.35%), crude fibre (5.38-11.07%), polysaccharides (33.85-46.79 mg g-1), total flavonoid content (TFC, 4.06-48.63 mg g-1) and total saponins (13.62-27.85 mg g-1).
30225063	3	12	theme	elata	549:553	arg1	buds					538:541	the buds	534:541	the buds of A. elata collected from eight different geographical regions (S1: Benxi; S2: Linjiang; S3: Pingwu; S4: Enshi; S5: Changbaishan; S6: Shangzhi; S7: Xiaoxinganling and S8: Harbin) in China	534:730	To better understand the nutritional value of A. elata, we compared total flavonoids, total saponins, phenolic compounds and mineral element contents in the buds of A. elata collected from eight different geographical regions (S1: Benxi; S2: Linjiang; S3: Pingwu; S4: Enshi; S5: Changbaishan; S6: Shangzhi; S7: Xiaoxinganling and S8: Harbin) in China.
30225063	1	13	theme	Aralia	109:114	arg1	buds					122:125	Aralia elata buds	109:125	Aralia elata buds	109:125	Aralia elata buds contain many nutrients and have a pleasant taste with a unique flavour.
30225063	4	14	theme	basic	761:765	arg1	composition					767:777	the basic composition	757:777	the basic composition in the buds	757:789	The results showed that the basic composition in the buds presented a wide variation, with ash (8.76-10.35%), crude fibre (5.38-11.07%), polysaccharides (33.85-46.79 mg g-1), total flavonoid content (TFC, 4.06-48.63 mg g-1) and total saponins (13.62-27.85 mg g-1).
30225063	6	15	from	content	1181:1187	arg1	Enshi					1192:1196	Enshi	1192:1196	Enshi	1192:1196	The total phenolic content in Enshi (S4) was significantly higher than others, and quercetin was the predominant phenolic compound in this sample.
30225063	6	15	from	content	1181:1187	arg1	S4					1199:1200	S4	1199:1200	S4	1199:1200	The total phenolic content in Enshi (S4) was significantly higher than others, and quercetin was the predominant phenolic compound in this sample.
30225063	3	16	theme	different	576:584	arg1	regions					599:605	eight different geographical regions	570:605	eight different geographical regions (S1: Benxi; S2: Linjiang; S3: Pingwu; S4: Enshi; S5: Changbaishan; S6: Shangzhi; S7: Xiaoxinganling and S8: Harbin) in China	570:730	To better understand the nutritional value of A. elata, we compared total flavonoids, total saponins, phenolic compounds and mineral element contents in the buds of A. elata collected from eight different geographical regions (S1: Benxi; S2: Linjiang; S3: Pingwu; S4: Enshi; S5: Changbaishan; S6: Shangzhi; S7: Xiaoxinganling and S8: Harbin) in China.
30225063	4	17	theme	4.06-48.63	938:947	arg1	mg					949:950	mg	949:950	mg	949:950	The results showed that the basic composition in the buds presented a wide variation, with ash (8.76-10.35%), crude fibre (5.38-11.07%), polysaccharides (33.85-46.79 mg g-1), total flavonoid content (TFC, 4.06-48.63 mg g-1) and total saponins (13.62-27.85 mg g-1).
30225063	5	18	theme	phenolic	1054:1061	arg1	analysis					1073:1080	the phenolic compounds analysis	1050:1080	the phenolic compounds analysis	1050:1080	UPLC combined with the LC-MS/MS method was used for the phenolic compounds analysis, and 11 phenolic compounds were identified and quantified in the eight samples.
30225063	0	19	theme	Aralia	50:55	arg1	elata					57:61	Aralia elata	50:61	Aralia elata from different geographical origins of China	50:106	Comparison of chemical composition in the buds of Aralia elata from different geographical origins of China.
30225063	10	20	located	found	1600:1604	arg1	S8					1609:1610	S8	1609:1610	S8	1609:1610	Moreover, the maximum estimated quantities of Ca and Sr were found in S8.
30225063	10	20	located	found	1600:1604	arg2	quantities					1571:1580	the maximum estimated quantities	1549:1580	the maximum estimated quantities of Ca and Sr	1549:1593	Moreover, the maximum estimated quantities of Ca and Sr were found in S8.
30225063	1	21	theme	many	135:138	arg1	nutrients					140:148	many nutrients	135:148	many nutrients	135:148	Aralia elata buds contain many nutrients and have a pleasant taste with a unique flavour.
30225063	9	22	theme	Mg	1472:1473	arg1	contents					1490:1497	maximum Mg, Mn, Co and Ni contents	1464:1497	maximum Mg, Mn, Co and Ni contents	1464:1497	We obtained maximum Mg, Mn, Co and Ni contents in S4, and found rich Zn content in S7.
30225063	5	23	used	used	1041:1044	arg2	UPLC					998:1001	UPLC	998:1001	UPLC combined with the LC-MS/MS method	998:1035	UPLC combined with the LC-MS/MS method was used for the phenolic compounds analysis, and 11 phenolic compounds were identified and quantified in the eight samples.
30225063	5	24	theme	LC-MS/MS	1021:1028	arg1	method					1030:1035	the LC-MS/MS method	1017:1035	the LC-MS/MS method	1017:1035	UPLC combined with the LC-MS/MS method was used for the phenolic compounds analysis, and 11 phenolic compounds were identified and quantified in the eight samples.
30225063	9	25	theme	Mn	1476:1477	arg1	contents					1490:1497	maximum Mg, Mn, Co and Ni contents	1464:1497	maximum Mg, Mn, Co and Ni contents	1464:1497	We obtained maximum Mg, Mn, Co and Ni contents in S4, and found rich Zn content in S7.
30225063	0	26	from	Comparison	0:9	arg1	buds					42:45	the buds	38:45	the buds of Aralia elata from different geographical origins of China	38:106	Comparison of chemical composition in the buds of Aralia elata from different geographical origins of China.
30225063	0	27	theme	geographical	78:89	arg1	origins					91:97	different geographical origins	68:97	different geographical origins of China	68:106	Comparison of chemical composition in the buds of Aralia elata from different geographical origins of China.
30225063	2	28	theme	root	261:264	arg1	bark					266:269	the root bark	257:269	the root bark of this species	257:285	Previous studies mainly focused on triterpene saponins in the root bark of this species, but little information existed concerning other chemical components, especially in the buds.
30225063	9	29	theme	Co	1480:1481	arg1	contents					1490:1497	maximum Mg, Mn, Co and Ni contents	1464:1497	maximum Mg, Mn, Co and Ni contents	1464:1497	We obtained maximum Mg, Mn, Co and Ni contents in S4, and found rich Zn content in S7.
30225063	0	30	from	buds	42:45	arg1	origins					91:97	different geographical origins	68:97	different geographical origins of China	68:106	Comparison of chemical composition in the buds of Aralia elata from different geographical origins of China.
30225063	5	31	theme	phenolic	1090:1097	arg1	compounds					1099:1107	11 phenolic compounds	1087:1107	11 phenolic compounds	1087:1107	UPLC combined with the LC-MS/MS method was used for the phenolic compounds analysis, and 11 phenolic compounds were identified and quantified in the eight samples.
30225063	3	32	theme	nutritional	406:416	arg1	value					418:422	the nutritional value	402:422	the nutritional value of A. elata	402:434	To better understand the nutritional value of A. elata, we compared total flavonoids, total saponins, phenolic compounds and mineral element contents in the buds of A. elata collected from eight different geographical regions (S1: Benxi; S2: Linjiang; S3: Pingwu; S4: Enshi; S5: Changbaishan; S6: Shangzhi; S7: Xiaoxinganling and S8: Harbin) in China.
30225063	9	33	theme	Ni	1487:1488	arg1	contents					1490:1497	maximum Mg, Mn, Co and Ni contents	1464:1497	maximum Mg, Mn, Co and Ni contents	1464:1497	We obtained maximum Mg, Mn, Co and Ni contents in S4, and found rich Zn content in S7.
30225063	4	34	theme	13.62-27.85	977:987	arg1	mg					989:990	mg	989:990	mg	989:990	The results showed that the basic composition in the buds presented a wide variation, with ash (8.76-10.35%), crude fibre (5.38-11.07%), polysaccharides (33.85-46.79 mg g-1), total flavonoid content (TFC, 4.06-48.63 mg g-1) and total saponins (13.62-27.85 mg g-1).
30225063	12	35	theme	production	1838:1847	arg1	field					1849:1853	the food production field	1829:1853	the food production field	1829:1853	Our results provided an essential theoretical basis of quality evaluation of A. elata buds in the food production field.
30225063	3	36	theme	elata	430:434	arg1	value					418:422	the nutritional value	402:422	the nutritional value of A. elata	402:434	To better understand the nutritional value of A. elata, we compared total flavonoids, total saponins, phenolic compounds and mineral element contents in the buds of A. elata collected from eight different geographical regions (S1: Benxi; S2: Linjiang; S3: Pingwu; S4: Enshi; S5: Changbaishan; S6: Shangzhi; S7: Xiaoxinganling and S8: Harbin) in China.
30225063	0	37	theme	chemical	14:21	arg1	composition					23:33	chemical composition	14:33	chemical composition	14:33	Comparison of chemical composition in the buds of Aralia elata from different geographical origins of China.
30225063	2	38	from	saponins	245:252	arg1	bark					266:269	the root bark	257:269	the root bark of this species	257:285	Previous studies mainly focused on triterpene saponins in the root bark of this species, but little information existed concerning other chemical components, especially in the buds.
30225063	12	39	theme	buds	1821:1824	arg1	evaluation					1798:1807	quality evaluation	1790:1807	quality evaluation of A. elata buds in the food production field	1790:1853	Our results provided an essential theoretical basis of quality evaluation of A. elata buds in the food production field.
30225063	6	40	theme	phenolic	1172:1179	arg1	higher					1221:1226	higher	1221:1226	higher	1221:1226	The total phenolic content in Enshi (S4) was significantly higher than others, and quercetin was the predominant phenolic compound in this sample.
30225063	6	40	theme	phenolic	1172:1179	arg1	content					1181:1187	The total phenolic content	1162:1187	The total phenolic content in Enshi (S4)	1162:1201	The total phenolic content in Enshi (S4) was significantly higher than others, and quercetin was the predominant phenolic compound in this sample.
30225063	1	41	contain	have	154:157	arg1	buds					122:125	Aralia elata buds	109:125	Aralia elata buds	109:125	Aralia elata buds contain many nutrients and have a pleasant taste with a unique flavour.
30225063	1	41	contain	have	154:157	arg2	taste					170:174	a pleasant taste	159:174	a pleasant taste with a unique flavour	159:196	Aralia elata buds contain many nutrients and have a pleasant taste with a unique flavour.
30225063	3	42	theme	total	449:453	arg1	flavonoids					455:464	total flavonoids	449:464	total flavonoids	449:464	To better understand the nutritional value of A. elata, we compared total flavonoids, total saponins, phenolic compounds and mineral element contents in the buds of A. elata collected from eight different geographical regions (S1: Benxi; S2: Linjiang; S3: Pingwu; S4: Enshi; S5: Changbaishan; S6: Shangzhi; S7: Xiaoxinganling and S8: Harbin) in China.
30225063	1	43	with	taste	170:174	arg1	flavour					190:196	a unique flavour	181:196	a unique flavour	181:196	Aralia elata buds contain many nutrients and have a pleasant taste with a unique flavour.
30225063	9	44	theme	Zn	1521:1522	arg1	content					1524:1530	rich Zn content	1516:1530	rich Zn content	1516:1530	We obtained maximum Mg, Mn, Co and Ni contents in S4, and found rich Zn content in S7.
30225063	12	45	theme	evaluation	1798:1807	arg1	basis					1781:1785	an essential theoretical basis	1756:1785	an essential theoretical basis of quality evaluation of A. elata buds in the food production field	1756:1853	Our results provided an essential theoretical basis of quality evaluation of A. elata buds in the food production field.
30225063	2	46	theme	little	292:297	arg1	information					299:309	little information	292:309	little information	292:309	Previous studies mainly focused on triterpene saponins in the root bark of this species, but little information existed concerning other chemical components, especially in the buds.
30225063	12	47	theme	A.	1812:1813	arg1	buds					1821:1824	A. elata buds	1812:1824	A. elata buds	1812:1824	Our results provided an essential theoretical basis of quality evaluation of A. elata buds in the food production field.
30225063	10	48	theme	Ca	1585:1586	arg1	quantities					1571:1580	the maximum estimated quantities	1549:1580	the maximum estimated quantities of Ca and Sr	1549:1593	Moreover, the maximum estimated quantities of Ca and Sr were found in S8.
30225063	2	49	theme	other	330:334	arg1	components					345:354	other chemical components	330:354	other chemical components	330:354	Previous studies mainly focused on triterpene saponins in the root bark of this species, but little information existed concerning other chemical components, especially in the buds.
30225063	11	50	from	composition	1652:1662	arg1	buds					1671:1674	the buds	1667:1674	the buds of A. elata	1667:1686	This study indicated that the chemical composition in the buds of A. elata was obviously affected by geographical origin.
30225063	10	51	theme	maximum	1553:1559	arg1	quantities					1571:1580	the maximum estimated quantities	1549:1580	the maximum estimated quantities of Ca and Sr	1549:1593	Moreover, the maximum estimated quantities of Ca and Sr were found in S8.
30225063	10	52	theme	Sr	1592:1593	arg1	quantities					1571:1580	the maximum estimated quantities	1549:1580	the maximum estimated quantities of Ca and Sr	1549:1593	Moreover, the maximum estimated quantities of Ca and Sr were found in S8.
30225063	3	53	theme	phenolic	483:490	arg1	compounds					492:500	phenolic compounds	483:500	phenolic compounds	483:500	To better understand the nutritional value of A. elata, we compared total flavonoids, total saponins, phenolic compounds and mineral element contents in the buds of A. elata collected from eight different geographical regions (S1: Benxi; S2: Linjiang; S3: Pingwu; S4: Enshi; S5: Changbaishan; S6: Shangzhi; S7: Xiaoxinganling and S8: Harbin) in China.
30225063	12	54	theme	essential	1759:1767	arg1	basis					1781:1785	an essential theoretical basis	1756:1785	an essential theoretical basis of quality evaluation of A. elata buds in the food production field	1756:1853	Our results provided an essential theoretical basis of quality evaluation of A. elata buds in the food production field.
30225063	1	55	contain	contain	127:133	arg2	nutrients					140:148	many nutrients	135:148	many nutrients	135:148	Aralia elata buds contain many nutrients and have a pleasant taste with a unique flavour.
30225063	1	55	contain	contain	127:133	arg1	buds					122:125	Aralia elata buds	109:125	Aralia elata buds	109:125	Aralia elata buds contain many nutrients and have a pleasant taste with a unique flavour.
30225063	6	56	theme	phenolic	1275:1282	arg1	compound					1284:1291	the predominant phenolic compound	1259:1291	the predominant phenolic compound	1259:1291	The total phenolic content in Enshi (S4) was significantly higher than others, and quercetin was the predominant phenolic compound in this sample.
30225063	6	56	theme	phenolic	1275:1282	arg1	quercetin					1245:1253	quercetin	1245:1253	quercetin	1245:1253	The total phenolic content in Enshi (S4) was significantly higher than others, and quercetin was the predominant phenolic compound in this sample.
30225063	1	57	theme	pleasant	161:168	arg1	taste					170:174	a pleasant taste	159:174	a pleasant taste with a unique flavour	159:196	Aralia elata buds contain many nutrients and have a pleasant taste with a unique flavour.
30225063	3	58	theme	element	514:520	arg1	contents					522:529	mineral element contents	506:529	mineral element contents	506:529	To better understand the nutritional value of A. elata, we compared total flavonoids, total saponins, phenolic compounds and mineral element contents in the buds of A. elata collected from eight different geographical regions (S1: Benxi; S2: Linjiang; S3: Pingwu; S4: Enshi; S5: Changbaishan; S6: Shangzhi; S7: Xiaoxinganling and S8: Harbin) in China.
30225063	12	59	from	field	1849:1853	arg1	basis					1781:1785	an essential theoretical basis	1756:1785	an essential theoretical basis of quality evaluation of A. elata buds in the food production field	1756:1853	Our results provided an essential theoretical basis of quality evaluation of A. elata buds in the food production field.
30225063	11	60	theme	A.	1679:1680	arg1	elata					1682:1686	A. elata	1679:1686	A. elata	1679:1686	This study indicated that the chemical composition in the buds of A. elata was obviously affected by geographical origin.
30225063	4	61	theme	flavonoid	914:922	arg1	content					924:930	total flavonoid content	908:930	total flavonoid content (TFC, 4.06-48.63 mg g-1)	908:955	The results showed that the basic composition in the buds presented a wide variation, with ash (8.76-10.35%), crude fibre (5.38-11.07%), polysaccharides (33.85-46.79 mg g-1), total flavonoid content (TFC, 4.06-48.63 mg g-1) and total saponins (13.62-27.85 mg g-1).
30225063	3	62	theme	A.	546:547	arg1	elata					549:553	A. elata	546:553	A. elata collected from eight different geographical regions (S1: Benxi; S2: Linjiang; S3: Pingwu; S4: Enshi; S5: Changbaishan; S6: Shangzhi; S7: Xiaoxinganling and S8: Harbin) in China	546:730	To better understand the nutritional value of A. elata, we compared total flavonoids, total saponins, phenolic compounds and mineral element contents in the buds of A. elata collected from eight different geographical regions (S1: Benxi; S2: Linjiang; S3: Pingwu; S4: Enshi; S5: Changbaishan; S6: Shangzhi; S7: Xiaoxinganling and S8: Harbin) in China.
30225063	1	63	theme	unique	183:188	arg1	flavour					190:196	a unique flavour	181:196	a unique flavour	181:196	Aralia elata buds contain many nutrients and have a pleasant taste with a unique flavour.
30225063	4	64	theme	33.85-46.79	887:897	arg1	mg					899:900	mg	899:900	mg	899:900	The results showed that the basic composition in the buds presented a wide variation, with ash (8.76-10.35%), crude fibre (5.38-11.07%), polysaccharides (33.85-46.79 mg g-1), total flavonoid content (TFC, 4.06-48.63 mg g-1) and total saponins (13.62-27.85 mg g-1).
30225063	7	65	theme	mineral	1355:1361	arg1	elements					1363:1370	nine mineral elements	1350:1370	nine mineral elements in the buds	1350:1382	We used ICP-OES to identify and quantify nine mineral elements in the buds.
30225063	4	66	theme	total	961:965	arg1	g-1					992:994	13.62-27.85 mg g-1	977:994	13.62-27.85 mg g-1	977:994	The results showed that the basic composition in the buds presented a wide variation, with ash (8.76-10.35%), crude fibre (5.38-11.07%), polysaccharides (33.85-46.79 mg g-1), total flavonoid content (TFC, 4.06-48.63 mg g-1) and total saponins (13.62-27.85 mg g-1).
30225063	4	66	theme	total	961:965	arg1	saponins					967:974	total saponins	961:974	total saponins (13.62-27.85 mg g-1)	961:995	The results showed that the basic composition in the buds presented a wide variation, with ash (8.76-10.35%), crude fibre (5.38-11.07%), polysaccharides (33.85-46.79 mg g-1), total flavonoid content (TFC, 4.06-48.63 mg g-1) and total saponins (13.62-27.85 mg g-1).
30225063	4	67	from	composition	767:777	arg1	buds					786:789	the buds	782:789	the buds	782:789	The results showed that the basic composition in the buds presented a wide variation, with ash (8.76-10.35%), crude fibre (5.38-11.07%), polysaccharides (33.85-46.79 mg g-1), total flavonoid content (TFC, 4.06-48.63 mg g-1) and total saponins (13.62-27.85 mg g-1).
30225063	2	68	theme	Previous	199:206	arg1	studies					208:214	Previous studies	199:214	Previous studies	199:214	Previous studies mainly focused on triterpene saponins in the root bark of this species, but little information existed concerning other chemical components, especially in the buds.
30225063	1	69	theme	elata	116:120	arg1	buds					122:125	Aralia elata buds	109:125	Aralia elata buds	109:125	Aralia elata buds contain many nutrients and have a pleasant taste with a unique flavour.
30225063	11	70	theme	geographical	1714:1725	arg1	origin					1727:1732	geographical origin	1714:1732	geographical origin	1714:1732	This study indicated that the chemical composition in the buds of A. elata was obviously affected by geographical origin.
30225063	0	71	from	origins	91:97	arg1	buds					42:45	the buds	38:45	the buds of Aralia elata from different geographical origins of China	38:106	Comparison of chemical composition in the buds of Aralia elata from different geographical origins of China.
30225063	0	71	from	origins	91:97	arg1	elata					57:61	Aralia elata	50:61	Aralia elata from different geographical origins of China	50:106	Comparison of chemical composition in the buds of Aralia elata from different geographical origins of China.
30225063	4	72	theme	mg	949:950	arg1	g-1					952:954	4.06-48.63 mg g-1	938:954	4.06-48.63 mg g-1	938:954	The results showed that the basic composition in the buds presented a wide variation, with ash (8.76-10.35%), crude fibre (5.38-11.07%), polysaccharides (33.85-46.79 mg g-1), total flavonoid content (TFC, 4.06-48.63 mg g-1) and total saponins (13.62-27.85 mg g-1).
30225063	4	72	theme	mg	949:950	arg1	TFC					933:935	TFC	933:935	TFC	933:935	The results showed that the basic composition in the buds presented a wide variation, with ash (8.76-10.35%), crude fibre (5.38-11.07%), polysaccharides (33.85-46.79 mg g-1), total flavonoid content (TFC, 4.06-48.63 mg g-1) and total saponins (13.62-27.85 mg g-1).
30225063	0	73	theme	elata	57:61	arg1	buds					42:45	the buds	38:45	the buds of Aralia elata from different geographical origins of China	38:106	Comparison of chemical composition in the buds of Aralia elata from different geographical origins of China.
30225063	9	74	theme	maximum	1464:1470	arg1	contents					1490:1497	maximum Mg, Mn, Co and Ni contents	1464:1497	maximum Mg, Mn, Co and Ni contents	1464:1497	We obtained maximum Mg, Mn, Co and Ni contents in S4, and found rich Zn content in S7.
30225063	2	75	theme	triterpene	234:243	arg1	saponins					245:252	triterpene saponins	234:252	triterpene saponins in the root bark of this species	234:285	Previous studies mainly focused on triterpene saponins in the root bark of this species, but little information existed concerning other chemical components, especially in the buds.
30225063	0	76	theme	different	68:76	arg1	origins					91:97	different geographical origins	68:97	different geographical origins of China	68:106	Comparison of chemical composition in the buds of Aralia elata from different geographical origins of China.
30225063	4	77	dep	content	924:930	arg1	g-1					952:954	4.06-48.63 mg g-1	938:954	4.06-48.63 mg g-1	938:954	The results showed that the basic composition in the buds presented a wide variation, with ash (8.76-10.35%), crude fibre (5.38-11.07%), polysaccharides (33.85-46.79 mg g-1), total flavonoid content (TFC, 4.06-48.63 mg g-1) and total saponins (13.62-27.85 mg g-1).
30225063	4	77	dep	content	924:930	arg1	TFC					933:935	TFC	933:935	TFC	933:935	The results showed that the basic composition in the buds presented a wide variation, with ash (8.76-10.35%), crude fibre (5.38-11.07%), polysaccharides (33.85-46.79 mg g-1), total flavonoid content (TFC, 4.06-48.63 mg g-1) and total saponins (13.62-27.85 mg g-1).
30225063	7	78	from	elements	1363:1370	arg1	buds					1379:1382	the buds	1375:1382	the buds	1375:1382	We used ICP-OES to identify and quantify nine mineral elements in the buds.
30225063	12	79	from	basis	1781:1785	arg1	field					1849:1853	the food production field	1829:1853	the food production field	1829:1853	Our results provided an essential theoretical basis of quality evaluation of A. elata buds in the food production field.
30225063	6	80	from	compound	1284:1291	arg1	sample					1301:1306	this sample	1296:1306	this sample	1296:1306	The total phenolic content in Enshi (S4) was significantly higher than others, and quercetin was the predominant phenolic compound in this sample.
30225063	0	81	theme	China	102:106	arg1	origins					91:97	different geographical origins	68:97	different geographical origins of China	68:106	Comparison of chemical composition in the buds of Aralia elata from different geographical origins of China.
30225063	4	82	theme	wide	803:806	arg1	variation					808:816	a wide variation	801:816	a wide variation	801:816	The results showed that the basic composition in the buds presented a wide variation, with ash (8.76-10.35%), crude fibre (5.38-11.07%), polysaccharides (33.85-46.79 mg g-1), total flavonoid content (TFC, 4.06-48.63 mg g-1) and total saponins (13.62-27.85 mg g-1).
30225063	7	83	used	used	1312:1315	arg2	We					1309:1310	We	1309:1310	We	1309:1310	We used ICP-OES to identify and quantify nine mineral elements in the buds.
30225063	12	84	theme	food	1833:1836	arg1	field					1849:1853	the food production field	1829:1853	the food production field	1829:1853	Our results provided an essential theoretical basis of quality evaluation of A. elata buds in the food production field.
30225063	5	85	theme	compounds	1063:1071	arg1	analysis					1073:1080	the phenolic compounds analysis	1050:1080	the phenolic compounds analysis	1050:1080	UPLC combined with the LC-MS/MS method was used for the phenolic compounds analysis, and 11 phenolic compounds were identified and quantified in the eight samples.
30225063	0	86	theme	composition	23:33	arg1	Comparison					0:9	Comparison	0:9	Comparison of chemical composition in the buds of Aralia elata from different geographical origins of China.	0:107	Comparison of chemical composition in the buds of Aralia elata from different geographical origins of China.
30225063	8	87	from	contents	1399:1406	arg1	S5					1411:1412	S5	1411:1412	S5	1411:1412	The Fe and Cu contents in S5 were much higher than that of others.
30225063	3	88	theme	geographical	586:597	arg1	regions					599:605	eight different geographical regions	570:605	eight different geographical regions (S1: Benxi; S2: Linjiang; S3: Pingwu; S4: Enshi; S5: Changbaishan; S6: Shangzhi; S7: Xiaoxinganling and S8: Harbin) in China	570:730	To better understand the nutritional value of A. elata, we compared total flavonoids, total saponins, phenolic compounds and mineral element contents in the buds of A. elata collected from eight different geographical regions (S1: Benxi; S2: Linjiang; S3: Pingwu; S4: Enshi; S5: Changbaishan; S6: Shangzhi; S7: Xiaoxinganling and S8: Harbin) in China.
30225063	12	89	theme	elata	1815:1819	arg1	buds					1821:1824	A. elata buds	1812:1824	A. elata buds	1812:1824	Our results provided an essential theoretical basis of quality evaluation of A. elata buds in the food production field.
30225063	3	90	theme	A.	427:428	arg1	elata					430:434	A. elata	427:434	A. elata	427:434	To better understand the nutritional value of A. elata, we compared total flavonoids, total saponins, phenolic compounds and mineral element contents in the buds of A. elata collected from eight different geographical regions (S1: Benxi; S2: Linjiang; S3: Pingwu; S4: Enshi; S5: Changbaishan; S6: Shangzhi; S7: Xiaoxinganling and S8: Harbin) in China.
30225063	6	91	theme	total	1166:1170	arg1	higher					1221:1226	higher	1221:1226	higher	1221:1226	The total phenolic content in Enshi (S4) was significantly higher than others, and quercetin was the predominant phenolic compound in this sample.
30225063	6	91	theme	total	1166:1170	arg1	content					1181:1187	The total phenolic content	1162:1187	The total phenolic content in Enshi (S4)	1162:1201	The total phenolic content in Enshi (S4) was significantly higher than others, and quercetin was the predominant phenolic compound in this sample.
30225063	9	92	theme	rich	1516:1519	arg1	content					1524:1530	rich Zn content	1516:1530	rich Zn content	1516:1530	We obtained maximum Mg, Mn, Co and Ni contents in S4, and found rich Zn content in S7.
30225063	8	93	theme	Fe	1389:1390	arg1	higher					1424:1429	higher	1424:1429	higher	1424:1429	The Fe and Cu contents in S5 were much higher than that of others.
30225063	8	93	theme	Fe	1389:1390	arg1	contents					1399:1406	The Fe and Cu contents	1385:1406	The Fe and Cu contents in S5	1385:1412	The Fe and Cu contents in S5 were much higher than that of others.
30225063	12	94	theme	quality	1790:1796	arg1	evaluation					1798:1807	quality evaluation	1790:1807	quality evaluation of A. elata buds in the food production field	1790:1853	Our results provided an essential theoretical basis of quality evaluation of A. elata buds in the food production field.
30225063	8	95	theme	Cu	1396:1397	arg1	higher					1424:1429	higher	1424:1429	higher	1424:1429	The Fe and Cu contents in S5 were much higher than that of others.
30225063	8	95	theme	Cu	1396:1397	arg1	contents					1399:1406	The Fe and Cu contents	1385:1406	The Fe and Cu contents in S5	1385:1412	The Fe and Cu contents in S5 were much higher than that of others.
30225063	3	96	dep	regions	599:605	arg1	S1					608:609	S1	608:609	S1	608:609	To better understand the nutritional value of A. elata, we compared total flavonoids, total saponins, phenolic compounds and mineral element contents in the buds of A. elata collected from eight different geographical regions (S1: Benxi; S2: Linjiang; S3: Pingwu; S4: Enshi; S5: Changbaishan; S6: Shangzhi; S7: Xiaoxinganling and S8: Harbin) in China.
30225063	2	97	theme	chemical	336:343	arg1	components					345:354	other chemical components	330:354	other chemical components	330:354	Previous studies mainly focused on triterpene saponins in the root bark of this species, but little information existed concerning other chemical components, especially in the buds.
30225063	3	98	dep	S1	608:609	arg1	Benxi					612:616	Benxi	612:616	Benxi	612:616	To better understand the nutritional value of A. elata, we compared total flavonoids, total saponins, phenolic compounds and mineral element contents in the buds of A. elata collected from eight different geographical regions (S1: Benxi; S2: Linjiang; S3: Pingwu; S4: Enshi; S5: Changbaishan; S6: Shangzhi; S7: Xiaoxinganling and S8: Harbin) in China.
30225063	3	98	dep	S1	608:609	arg1	S2					619:620	S2	619:620	S2: Linjiang; S3: Pingwu; S4: Enshi; S5: Changbaishan; S6: Shangzhi; S7: Xiaoxinganling and S8: Harbin	619:720	To better understand the nutritional value of A. elata, we compared total flavonoids, total saponins, phenolic compounds and mineral element contents in the buds of A. elata collected from eight different geographical regions (S1: Benxi; S2: Linjiang; S3: Pingwu; S4: Enshi; S5: Changbaishan; S6: Shangzhi; S7: Xiaoxinganling and S8: Harbin) in China.
31637462	10	0	from	direction	1852:1860	arg1	estimation					1807:1816	the estimation	1803:1816	the estimation of biofilm growth in the vertical direction	1803:1860	In addition, nanoFTIR and AFM investigations allowed the estimation of biofilm growth in the vertical direction and the morphological analysis of bacterial colonies at different time points and the evaluation of the chemical composition at the nanoscale.
31637462	10	0	from	direction	1852:1860	arg1	analysis					1884:1891	the morphological analysis	1866:1891	the morphological analysis of bacterial colonies	1866:1913	In addition, nanoFTIR and AFM investigations allowed the estimation of biofilm growth in the vertical direction and the morphological analysis of bacterial colonies at different time points and the evaluation of the chemical composition at the nanoscale.
31637462	4	1	theme	architecture	612:623	arg1	details					597:603	details	597:603	details of its architecture including the spatial distribution of the macromolecular components which might give hints on the way the biofilm scaffold is built up by bacteria	597:770	Due to the complexity of the matrix, few information is present in the literature on details of its architecture including the spatial distribution of the macromolecular components which might give hints on the way the biofilm scaffold is built up by bacteria.
31637462	0	2	dep	Klebsiella	83:92	arg1	clinical					105:112	clinical	105:112	clinical	105:112	Multi-technique microscopy investigation on bacterial biofilm matrices: a study on Klebsiella pneumoniae clinical strains.
31637462	0	2	dep	Klebsiella	83:92	arg1	pneumoniae					94:103	pneumoniae	94:103	pneumoniae	94:103	Multi-technique microscopy investigation on bacterial biofilm matrices: a study on Klebsiella pneumoniae clinical strains.
31637462	10	3	theme	time	1928:1931	arg1	points					1933:1938	different time points	1918:1938	different time points	1918:1938	In addition, nanoFTIR and AFM investigations allowed the estimation of biofilm growth in the vertical direction and the morphological analysis of bacterial colonies at different time points and the evaluation of the chemical composition at the nanoscale.
31637462	2	4	theme	numerous	290:297	arg1	advantages					299:308	numerous advantages	290:308	numerous advantages	290:308	This life style confers numerous advantages to bacteria including protection against external threats.
31637462	4	5	from	literature	583:592	arg1	details					597:603	details	597:603	details of its architecture including the spatial distribution of the macromolecular components which might give hints on the way the biofilm scaffold is built up by bacteria	597:770	Due to the complexity of the matrix, few information is present in the literature on details of its architecture including the spatial distribution of the macromolecular components which might give hints on the way the biofilm scaffold is built up by bacteria.
31637462	4	5	from	literature	583:592	arg1	present					568:574	present	568:574	present	568:574	Due to the complexity of the matrix, few information is present in the literature on details of its architecture including the spatial distribution of the macromolecular components which might give hints on the way the biofilm scaffold is built up by bacteria.
31637462	10	6	from	analysis	1884:1891	arg1	direction					1852:1860	the vertical direction	1839:1860	the vertical direction	1839:1860	In addition, nanoFTIR and AFM investigations allowed the estimation of biofilm growth in the vertical direction and the morphological analysis of bacterial colonies at different time points and the evaluation of the chemical composition at the nanoscale.
31637462	9	7	theme	multi-layered	1649:1661	arg1	structure					1663:1671	a 3-dimensional multi-layered structure	1633:1671	a 3-dimensional multi-layered structure	1633:1671	It was also possible to follow the time course of biofilm from 6 up to 48 h when the biofilm grew into a 3-dimensional multi-layered structure, characteristic of colonies of bacteria linked together by a complex matrix.
31637462	6	8	theme	Klebsiella	1185:1194	arg1	pneumoniae					1196:1205	Klebsiella pneumoniae	1185:1205	Klebsiella pneumoniae	1185:1205	To this, confocal microscopy, diffraction-limited infrared (IR) spectral imaging, and atomic force microscopy (AFM) were used to explore biofilm produced by a clinical strain of Klebsiella pneumoniae.
31637462	6	9	theme	confocal	1016:1023	arg1	microscopy					1025:1034	confocal microscopy	1016:1034	confocal microscopy	1016:1034	To this, confocal microscopy, diffraction-limited infrared (IR) spectral imaging, and atomic force microscopy (AFM) were used to explore biofilm produced by a clinical strain of Klebsiella pneumoniae.
31637462	4	10	attach	present	568:574	arg1	literature					583:592	the literature	579:592	the literature on details of its architecture including the spatial distribution of the macromolecular components which might give hints on the way the biofilm scaffold is built up by bacteria	579:770	Due to the complexity of the matrix, few information is present in the literature on details of its architecture including the spatial distribution of the macromolecular components which might give hints on the way the biofilm scaffold is built up by bacteria.
31637462	4	10	attach	present	568:574	arg2	information					553:563	few information	549:563	few information	549:563	Due to the complexity of the matrix, few information is present in the literature on details of its architecture including the spatial distribution of the macromolecular components which might give hints on the way the biofilm scaffold is built up by bacteria.
31637462	4	11	theme	components	682:691	arg1	distribution					647:658	the spatial distribution	635:658	the spatial distribution of the macromolecular components which might give hints on the way the biofilm scaffold is built up by bacteria	635:770	Due to the complexity of the matrix, few information is present in the literature on details of its architecture including the spatial distribution of the macromolecular components which might give hints on the way the biofilm scaffold is built up by bacteria.
31637462	10	12	theme	composition	1975:1985	arg1	points					1933:1938	different time points	1918:1938	different time points	1918:1938	In addition, nanoFTIR and AFM investigations allowed the estimation of biofilm growth in the vertical direction and the morphological analysis of bacterial colonies at different time points and the evaluation of the chemical composition at the nanoscale.
31637462	10	12	theme	composition	1975:1985	arg1	evaluation					1948:1957	the evaluation	1944:1957	the evaluation of the chemical composition at the nanoscale	1944:2002	In addition, nanoFTIR and AFM investigations allowed the estimation of biofilm growth in the vertical direction and the morphological analysis of bacterial colonies at different time points and the evaluation of the chemical composition at the nanoscale.
31637462	10	13	theme	AFM	1776:1778	arg1	investigations					1780:1793	AFM investigations	1776:1793	AFM investigations	1776:1793	In addition, nanoFTIR and AFM investigations allowed the estimation of biofilm growth in the vertical direction and the morphological analysis of bacterial colonies at different time points and the evaluation of the chemical composition at the nanoscale.
31637462	5	14	theme	macromolecular	960:973	arg1	components					975:984	the macromolecular components	956:984	the macromolecular components of biofilm matrices	956:1004	In this study, we investigated the possibility to combine well-established microbiological procedures with advanced microscopies to get information on composition and distribution of the macromolecular components of biofilm matrices.
31637462	5	15	from	information	909:919	arg1	distribution					940:951	distribution	940:951	distribution	940:951	In this study, we investigated the possibility to combine well-established microbiological procedures with advanced microscopies to get information on composition and distribution of the macromolecular components of biofilm matrices.
31637462	5	15	from	information	909:919	arg1	composition					924:934	composition	924:934	composition	924:934	In this study, we investigated the possibility to combine well-established microbiological procedures with advanced microscopies to get information on composition and distribution of the macromolecular components of biofilm matrices.
31637462	6	16	theme	spectral	1071:1078	arg1	imaging					1080:1086	diffraction-limited infrared (IR) spectral imaging	1037:1086	diffraction-limited infrared (IR) spectral imaging	1037:1086	To this, confocal microscopy, diffraction-limited infrared (IR) spectral imaging, and atomic force microscopy (AFM) were used to explore biofilm produced by a clinical strain of Klebsiella pneumoniae.
31637462	8	17	theme	matrix	1485:1490	arg1	composition					1455:1465	the chemical and structural composition	1427:1465	the chemical and structural composition of the saccaridic matrix	1427:1490	Through the analysis of the pure component spectra, it was possible to assess the chemical and structural composition of the saccaridic matrix, confirming the data obtained by NMR.
31637462	6	18	theme	IR	1067:1068	arg1	imaging					1080:1086	diffraction-limited infrared (IR) spectral imaging	1037:1086	diffraction-limited infrared (IR) spectral imaging	1037:1086	To this, confocal microscopy, diffraction-limited infrared (IR) spectral imaging, and atomic force microscopy (AFM) were used to explore biofilm produced by a clinical strain of Klebsiella pneumoniae.
31637462	9	19	theme	complex	1734:1740	arg1	matrix					1742:1747	a complex matrix	1732:1747	a complex matrix	1732:1747	It was also possible to follow the time course of biofilm from 6 up to 48 h when the biofilm grew into a 3-dimensional multi-layered structure, characteristic of colonies of bacteria linked together by a complex matrix.
31637462	0	20	from	investigation	27:39	arg1	matrices					62:69	bacterial biofilm matrices	44:69	bacterial biofilm matrices	44:69	Multi-technique microscopy investigation on bacterial biofilm matrices: a study on Klebsiella pneumoniae clinical strains.
31637462	1	21	from	embedded	167:174	arg1	matrix					197:202	a highly hydrated matrix	179:202	a highly hydrated matrix composed of polysaccharides, proteins, and extracellular DNA	179:263	Biofilms are communities of bacteria living embedded in a highly hydrated matrix composed of polysaccharides, proteins, and extracellular DNA.
31637462	6	22	theme	infrared	1057:1064	arg1	imaging					1080:1086	diffraction-limited infrared (IR) spectral imaging	1037:1086	diffraction-limited infrared (IR) spectral imaging	1037:1086	To this, confocal microscopy, diffraction-limited infrared (IR) spectral imaging, and atomic force microscopy (AFM) were used to explore biofilm produced by a clinical strain of Klebsiella pneumoniae.
31637462	4	23	theme	matrix	541:546	arg1	complexity					523:532	the complexity	519:532	the complexity of the matrix	519:546	Due to the complexity of the matrix, few information is present in the literature on details of its architecture including the spatial distribution of the macromolecular components which might give hints on the way the biofilm scaffold is built up by bacteria.
31637462	4	24	located	present	568:574	arg1	literature					583:592	the literature	579:592	the literature on details of its architecture including the spatial distribution of the macromolecular components which might give hints on the way the biofilm scaffold is built up by bacteria	579:770	Due to the complexity of the matrix, few information is present in the literature on details of its architecture including the spatial distribution of the macromolecular components which might give hints on the way the biofilm scaffold is built up by bacteria.
31637462	4	24	located	present	568:574	arg2	information					553:563	few information	549:563	few information	549:563	Due to the complexity of the matrix, few information is present in the literature on details of its architecture including the spatial distribution of the macromolecular components which might give hints on the way the biofilm scaffold is built up by bacteria.
31637462	4	25	dep	way	723:725	arg1	built					751:755	built	751:755	is built up by bacteria	748:770	Due to the complexity of the matrix, few information is present in the literature on details of its architecture including the spatial distribution of the macromolecular components which might give hints on the way the biofilm scaffold is built up by bacteria.
31637462	0	26	theme	biofilm	54:60	arg1	matrices					62:69	bacterial biofilm matrices	44:69	bacterial biofilm matrices	44:69	Multi-technique microscopy investigation on bacterial biofilm matrices: a study on Klebsiella pneumoniae clinical strains.
31637462	8	27	theme	pure	1377:1380	arg1	spectra					1392:1398	the pure component spectra	1373:1398	the pure component spectra	1373:1398	Through the analysis of the pure component spectra, it was possible to assess the chemical and structural composition of the saccaridic matrix, confirming the data obtained by NMR.
31637462	0	28	theme	Multi-technique	0:14	arg1	investigation					27:39	Multi-technique microscopy investigation	0:39	Multi-technique microscopy investigation on bacterial biofilm matrices: a study on Klebsiella pneumoniae clinical strains.	0:121	Multi-technique microscopy investigation on bacterial biofilm matrices: a study on Klebsiella pneumoniae clinical strains.
31637462	5	29	theme	matrices	997:1004	arg1	components					975:984	the macromolecular components	956:984	the macromolecular components of biofilm matrices	956:1004	In this study, we investigated the possibility to combine well-established microbiological procedures with advanced microscopies to get information on composition and distribution of the macromolecular components of biofilm matrices.
31637462	8	30	theme	spectra	1392:1398	arg1	analysis					1361:1368	the analysis	1357:1368	the analysis of the pure component spectra	1357:1398	Through the analysis of the pure component spectra, it was possible to assess the chemical and structural composition of the saccaridic matrix, confirming the data obtained by NMR.
31637462	5	31	theme	microbiological	848:862	arg1	procedures					864:873	well-established microbiological procedures	831:873	well-established microbiological procedures	831:873	In this study, we investigated the possibility to combine well-established microbiological procedures with advanced microscopies to get information on composition and distribution of the macromolecular components of biofilm matrices.
31637462	6	32	theme	atomic	1093:1098	arg1	AFM					1118:1120	AFM	1118:1120	AFM	1118:1120	To this, confocal microscopy, diffraction-limited infrared (IR) spectral imaging, and atomic force microscopy (AFM) were used to explore biofilm produced by a clinical strain of Klebsiella pneumoniae.
31637462	6	32	theme	atomic	1093:1098	arg1	microscopy					1106:1115	atomic force microscopy	1093:1115	atomic force microscopy (AFM)	1093:1121	To this, confocal microscopy, diffraction-limited infrared (IR) spectral imaging, and atomic force microscopy (AFM) were used to explore biofilm produced by a clinical strain of Klebsiella pneumoniae.
31637462	0	33	theme	bacterial	44:52	arg1	matrices					62:69	bacterial biofilm matrices	44:69	bacterial biofilm matrices	44:69	Multi-technique microscopy investigation on bacterial biofilm matrices: a study on Klebsiella pneumoniae clinical strains.
31637462	10	34	from	nanoscale	1994:2002	arg1	points					1933:1938	different time points	1918:1938	different time points	1918:1938	In addition, nanoFTIR and AFM investigations allowed the estimation of biofilm growth in the vertical direction and the morphological analysis of bacterial colonies at different time points and the evaluation of the chemical composition at the nanoscale.
31637462	10	34	from	nanoscale	1994:2002	arg1	evaluation					1948:1957	the evaluation	1944:1957	the evaluation of the chemical composition at the nanoscale	1944:2002	In addition, nanoFTIR and AFM investigations allowed the estimation of biofilm growth in the vertical direction and the morphological analysis of bacterial colonies at different time points and the evaluation of the chemical composition at the nanoscale.
31637462	9	35	theme	biofilm	1580:1586	arg1	course					1570:1575	the time course	1561:1575	the time course of biofilm from 6	1561:1593	It was also possible to follow the time course of biofilm from 6 up to 48 h when the biofilm grew into a 3-dimensional multi-layered structure, characteristic of colonies of bacteria linked together by a complex matrix.
31637462	4	36	theme	few	549:551	arg1	information					553:563	few information	549:563	few information	549:563	Due to the complexity of the matrix, few information is present in the literature on details of its architecture including the spatial distribution of the macromolecular components which might give hints on the way the biofilm scaffold is built up by bacteria.
31637462	10	37	theme	biofilm	1821:1827	arg1	growth					1829:1834	biofilm growth	1821:1834	biofilm growth in the vertical direction	1821:1860	In addition, nanoFTIR and AFM investigations allowed the estimation of biofilm growth in the vertical direction and the morphological analysis of bacterial colonies at different time points and the evaluation of the chemical composition at the nanoscale.
31637462	7	38	contain	have	1232:1235	arg2	clues					1237:1241	clues	1237:1241	clues	1237:1241	IR imaging permitted to have clues on how the biofilm grows and spreads on surfaces, and the local distribution of the components within it.
31637462	7	38	contain	have	1232:1235	arg1	imaging					1211:1217	IR imaging	1208:1217	IR imaging	1208:1217	IR imaging permitted to have clues on how the biofilm grows and spreads on surfaces, and the local distribution of the components within it.
31637462	2	39	theme	external	351:358	arg1	threats					360:366	external threats	351:366	external threats	351:366	This life style confers numerous advantages to bacteria including protection against external threats.
31637462	0	40	dep	investigation	27:39	arg1	study					74:78	a study	72:78	Multi-technique microscopy investigation on bacterial biofilm matrices: a study on Klebsiella pneumoniae clinical strains.	0:121	Multi-technique microscopy investigation on bacterial biofilm matrices: a study on Klebsiella pneumoniae clinical strains.
31637462	10	41	theme	vertical	1843:1850	arg1	direction					1852:1860	the vertical direction	1839:1860	the vertical direction	1839:1860	In addition, nanoFTIR and AFM investigations allowed the estimation of biofilm growth in the vertical direction and the morphological analysis of bacterial colonies at different time points and the evaluation of the chemical composition at the nanoscale.
31637462	8	42	theme	chemical	1431:1438	arg1	composition					1455:1465	the chemical and structural composition	1427:1465	the chemical and structural composition of the saccaridic matrix	1427:1490	Through the analysis of the pure component spectra, it was possible to assess the chemical and structural composition of the saccaridic matrix, confirming the data obtained by NMR.
31637462	4	43	theme	spatial	639:645	arg1	distribution					647:658	the spatial distribution	635:658	the spatial distribution of the macromolecular components which might give hints on the way the biofilm scaffold is built up by bacteria	635:770	Due to the complexity of the matrix, few information is present in the literature on details of its architecture including the spatial distribution of the macromolecular components which might give hints on the way the biofilm scaffold is built up by bacteria.
31637462	3	44	from	relevant	478:485	arg1	infections					500:509	dangerous infections	490:509	dangerous infections	490:509	However, they also contribute to increase bacterial resistance against antimicrobials, an issue particularly relevant in dangerous infections.
31637462	10	45	theme	morphological	1870:1882	arg1	analysis					1884:1891	the morphological analysis	1866:1891	the morphological analysis of bacterial colonies	1866:1913	In addition, nanoFTIR and AFM investigations allowed the estimation of biofilm growth in the vertical direction and the morphological analysis of bacterial colonies at different time points and the evaluation of the chemical composition at the nanoscale.
31637462	10	46	theme	different	1918:1926	arg1	points					1933:1938	different time points	1918:1938	different time points	1918:1938	In addition, nanoFTIR and AFM investigations allowed the estimation of biofilm growth in the vertical direction and the morphological analysis of bacterial colonies at different time points and the evaluation of the chemical composition at the nanoscale.
31637462	9	47	theme	3-dimensional	1635:1647	arg1	structure					1663:1671	a 3-dimensional multi-layered structure	1633:1671	a 3-dimensional multi-layered structure	1633:1671	It was also possible to follow the time course of biofilm from 6 up to 48 h when the biofilm grew into a 3-dimensional multi-layered structure, characteristic of colonies of bacteria linked together by a complex matrix.
31637462	6	48	theme	pneumoniae	1196:1205	arg1	strain					1175:1180	a clinical strain	1164:1180	a clinical strain of Klebsiella pneumoniae	1164:1205	To this, confocal microscopy, diffraction-limited infrared (IR) spectral imaging, and atomic force microscopy (AFM) were used to explore biofilm produced by a clinical strain of Klebsiella pneumoniae.
31637462	10	49	theme	colonies	1906:1913	arg1	estimation					1807:1816	the estimation	1803:1816	the estimation of biofilm growth in the vertical direction	1803:1860	In addition, nanoFTIR and AFM investigations allowed the estimation of biofilm growth in the vertical direction and the morphological analysis of bacterial colonies at different time points and the evaluation of the chemical composition at the nanoscale.
31637462	10	49	theme	colonies	1906:1913	arg1	analysis					1884:1891	the morphological analysis	1866:1891	the morphological analysis of bacterial colonies	1866:1913	In addition, nanoFTIR and AFM investigations allowed the estimation of biofilm growth in the vertical direction and the morphological analysis of bacterial colonies at different time points and the evaluation of the chemical composition at the nanoscale.
31637462	1	50	theme	hydrated	188:195	arg1	matrix					197:202	a highly hydrated matrix	179:202	a highly hydrated matrix composed of polysaccharides, proteins, and extracellular DNA	179:263	Biofilms are communities of bacteria living embedded in a highly hydrated matrix composed of polysaccharides, proteins, and extracellular DNA.
31637462	9	51	theme	characteristic	1674:1687	arg1	structure					1663:1671	a 3-dimensional multi-layered structure	1633:1671	a 3-dimensional multi-layered structure	1633:1671	It was also possible to follow the time course of biofilm from 6 up to 48 h when the biofilm grew into a 3-dimensional multi-layered structure, characteristic of colonies of bacteria linked together by a complex matrix.
31637462	8	52	theme	structural	1444:1453	arg1	composition					1455:1465	the chemical and structural composition	1427:1465	the chemical and structural composition of the saccaridic matrix	1427:1490	Through the analysis of the pure component spectra, it was possible to assess the chemical and structural composition of the saccaridic matrix, confirming the data obtained by NMR.
31637462	6	53	theme	clinical	1166:1173	arg1	strain					1175:1180	a clinical strain	1164:1180	a clinical strain of Klebsiella pneumoniae	1164:1205	To this, confocal microscopy, diffraction-limited infrared (IR) spectral imaging, and atomic force microscopy (AFM) were used to explore biofilm produced by a clinical strain of Klebsiella pneumoniae.
31637462	5	54	theme	advanced	880:887	arg1	microscopies					889:900	advanced microscopies	880:900	advanced microscopies	880:900	In this study, we investigated the possibility to combine well-established microbiological procedures with advanced microscopies to get information on composition and distribution of the macromolecular components of biofilm matrices.
31637462	9	55	theme	colonies	1692:1699	arg1	characteristic					1674:1687	characteristic	1674:1687	characteristic	1674:1687	It was also possible to follow the time course of biofilm from 6 up to 48 h when the biofilm grew into a 3-dimensional multi-layered structure, characteristic of colonies of bacteria linked together by a complex matrix.
31637462	0	56	from	study	74:78	arg1	strains					114:120	Klebsiella pneumoniae clinical strains	83:120	Klebsiella pneumoniae clinical strains	83:120	Multi-technique microscopy investigation on bacterial biofilm matrices: a study on Klebsiella pneumoniae clinical strains.
31637462	4	57	theme	macromolecular	667:680	arg1	components					682:691	the macromolecular components	663:691	the macromolecular components which might give hints on the way the biofilm scaffold is built up by bacteria	663:770	Due to the complexity of the matrix, few information is present in the literature on details of its architecture including the spatial distribution of the macromolecular components which might give hints on the way the biofilm scaffold is built up by bacteria.
31637462	10	58	theme	chemical	1966:1973	arg1	composition					1975:1985	the chemical composition	1962:1985	the chemical composition	1962:1985	In addition, nanoFTIR and AFM investigations allowed the estimation of biofilm growth in the vertical direction and the morphological analysis of bacterial colonies at different time points and the evaluation of the chemical composition at the nanoscale.
31637462	6	59	used	used	1128:1131	arg2	microscopy					1025:1034	confocal microscopy	1016:1034	confocal microscopy	1016:1034	To this, confocal microscopy, diffraction-limited infrared (IR) spectral imaging, and atomic force microscopy (AFM) were used to explore biofilm produced by a clinical strain of Klebsiella pneumoniae.
31637462	6	59	used	used	1128:1131	arg2	imaging					1080:1086	diffraction-limited infrared (IR) spectral imaging	1037:1086	diffraction-limited infrared (IR) spectral imaging	1037:1086	To this, confocal microscopy, diffraction-limited infrared (IR) spectral imaging, and atomic force microscopy (AFM) were used to explore biofilm produced by a clinical strain of Klebsiella pneumoniae.
31637462	6	59	used	used	1128:1131	arg2	AFM					1118:1120	AFM	1118:1120	AFM	1118:1120	To this, confocal microscopy, diffraction-limited infrared (IR) spectral imaging, and atomic force microscopy (AFM) were used to explore biofilm produced by a clinical strain of Klebsiella pneumoniae.
31637462	6	59	used	used	1128:1131	arg2	microscopy					1106:1115	atomic force microscopy	1093:1115	atomic force microscopy (AFM)	1093:1121	To this, confocal microscopy, diffraction-limited infrared (IR) spectral imaging, and atomic force microscopy (AFM) were used to explore biofilm produced by a clinical strain of Klebsiella pneumoniae.
31637462	9	60	theme	bacteria	1704:1711	arg1	colonies					1692:1699	colonies	1692:1699	colonies of bacteria linked together by a complex matrix	1692:1747	It was also possible to follow the time course of biofilm from 6 up to 48 h when the biofilm grew into a 3-dimensional multi-layered structure, characteristic of colonies of bacteria linked together by a complex matrix.
31637462	8	61	theme	saccaridic	1474:1483	arg1	matrix					1485:1490	the saccaridic matrix	1470:1490	the saccaridic matrix	1470:1490	Through the analysis of the pure component spectra, it was possible to assess the chemical and structural composition of the saccaridic matrix, confirming the data obtained by NMR.
31637462	4	62	theme	biofilm	731:737	arg1	scaffold					739:746	the biofilm scaffold	727:746	the biofilm scaffold	727:746	Due to the complexity of the matrix, few information is present in the literature on details of its architecture including the spatial distribution of the macromolecular components which might give hints on the way the biofilm scaffold is built up by bacteria.
31637462	2	63	theme	life	271:274	arg1	style					276:280	This life style	266:280	This life style	266:280	This life style confers numerous advantages to bacteria including protection against external threats.
31637462	5	64	theme	components	975:984	arg1	distribution					940:951	distribution	940:951	distribution	940:951	In this study, we investigated the possibility to combine well-established microbiological procedures with advanced microscopies to get information on composition and distribution of the macromolecular components of biofilm matrices.
31637462	5	64	theme	components	975:984	arg1	composition					924:934	composition	924:934	composition	924:934	In this study, we investigated the possibility to combine well-established microbiological procedures with advanced microscopies to get information on composition and distribution of the macromolecular components of biofilm matrices.
31637462	9	65	from	course	1570:1575	arg1	6					1593:1593	6	1593:1593	6	1593:1593	It was also possible to follow the time course of biofilm from 6 up to 48 h when the biofilm grew into a 3-dimensional multi-layered structure, characteristic of colonies of bacteria linked together by a complex matrix.
31637462	1	66	from	matrix	197:202	arg1	embedded					167:174	embedded	167:174	embedded	167:174	Biofilms are communities of bacteria living embedded in a highly hydrated matrix composed of polysaccharides, proteins, and extracellular DNA.
31637462	1	67	theme	bacteria	151:158	arg1	communities					136:146	communities	136:146	communities of bacteria living embedded in a highly hydrated matrix composed of polysaccharides, proteins, and extracellular DNA	136:263	Biofilms are communities of bacteria living embedded in a highly hydrated matrix composed of polysaccharides, proteins, and extracellular DNA.
31637462	1	67	theme	bacteria	151:158	arg1	Biofilms					123:130	Biofilms	123:130	Biofilms	123:130	Biofilms are communities of bacteria living embedded in a highly hydrated matrix composed of polysaccharides, proteins, and extracellular DNA.
31637462	0	68	theme	Klebsiella	83:92	arg1	strains					114:120	Klebsiella pneumoniae clinical strains	83:120	Klebsiella pneumoniae clinical strains	83:120	Multi-technique microscopy investigation on bacterial biofilm matrices: a study on Klebsiella pneumoniae clinical strains.
31637462	6	69	theme	diffraction-limited	1037:1055	arg1	imaging					1080:1086	diffraction-limited infrared (IR) spectral imaging	1037:1086	diffraction-limited infrared (IR) spectral imaging	1037:1086	To this, confocal microscopy, diffraction-limited infrared (IR) spectral imaging, and atomic force microscopy (AFM) were used to explore biofilm produced by a clinical strain of Klebsiella pneumoniae.
31637462	3	70	theme	relevant	478:485	arg1	antimicrobials					440:453	antimicrobials	440:453	antimicrobials	440:453	However, they also contribute to increase bacterial resistance against antimicrobials, an issue particularly relevant in dangerous infections.
31637462	3	70	theme	relevant	478:485	arg1	issue					459:463	an issue	456:463	an issue particularly relevant in dangerous infections	456:509	However, they also contribute to increase bacterial resistance against antimicrobials, an issue particularly relevant in dangerous infections.
31637462	0	71	theme	microscopy	16:25	arg1	investigation					27:39	Multi-technique microscopy investigation	0:39	Multi-technique microscopy investigation on bacterial biofilm matrices: a study on Klebsiella pneumoniae clinical strains.	0:121	Multi-technique microscopy investigation on bacterial biofilm matrices: a study on Klebsiella pneumoniae clinical strains.
31637462	5	72	theme	biofilm	989:995	arg1	matrices					997:1004	biofilm matrices	989:1004	biofilm matrices	989:1004	In this study, we investigated the possibility to combine well-established microbiological procedures with advanced microscopies to get information on composition and distribution of the macromolecular components of biofilm matrices.
31637462	7	73	theme	components	1327:1336	arg1	distribution					1307:1318	the local distribution	1297:1318	the local distribution of the components within it	1297:1346	IR imaging permitted to have clues on how the biofilm grows and spreads on surfaces, and the local distribution of the components within it.
31637462	7	73	theme	components	1327:1336	arg1	surfaces					1283:1290	surfaces	1283:1290	surfaces	1283:1290	IR imaging permitted to have clues on how the biofilm grows and spreads on surfaces, and the local distribution of the components within it.
31637462	3	74	theme	bacterial	411:419	arg1	resistance					421:430	bacterial resistance	411:430	bacterial resistance against antimicrobials, an issue particularly relevant in dangerous infections	411:509	However, they also contribute to increase bacterial resistance against antimicrobials, an issue particularly relevant in dangerous infections.
31637462	5	75	theme	well-established	831:846	arg1	procedures					864:873	well-established microbiological procedures	831:873	well-established microbiological procedures	831:873	In this study, we investigated the possibility to combine well-established microbiological procedures with advanced microscopies to get information on composition and distribution of the macromolecular components of biofilm matrices.
31637462	9	76	theme	time	1565:1568	arg1	course					1570:1575	the time course	1561:1575	the time course of biofilm from 6	1561:1593	It was also possible to follow the time course of biofilm from 6 up to 48 h when the biofilm grew into a 3-dimensional multi-layered structure, characteristic of colonies of bacteria linked together by a complex matrix.
31637462	9	77	from	6	1593:1593	arg1	biofilm					1580:1586	biofilm	1580:1586	biofilm from 6	1580:1593	It was also possible to follow the time course of biofilm from 6 up to 48 h when the biofilm grew into a 3-dimensional multi-layered structure, characteristic of colonies of bacteria linked together by a complex matrix.
31637462	9	77	from	6	1593:1593	arg1	course					1570:1575	the time course	1561:1575	the time course of biofilm from 6	1561:1593	It was also possible to follow the time course of biofilm from 6 up to 48 h when the biofilm grew into a 3-dimensional multi-layered structure, characteristic of colonies of bacteria linked together by a complex matrix.
31637462	6	78	theme	force	1100:1104	arg1	AFM					1118:1120	AFM	1118:1120	AFM	1118:1120	To this, confocal microscopy, diffraction-limited infrared (IR) spectral imaging, and atomic force microscopy (AFM) were used to explore biofilm produced by a clinical strain of Klebsiella pneumoniae.
31637462	6	78	theme	force	1100:1104	arg1	microscopy					1106:1115	atomic force microscopy	1093:1115	atomic force microscopy (AFM)	1093:1121	To this, confocal microscopy, diffraction-limited infrared (IR) spectral imaging, and atomic force microscopy (AFM) were used to explore biofilm produced by a clinical strain of Klebsiella pneumoniae.
31637462	8	79	theme	component	1382:1390	arg1	spectra					1392:1398	the pure component spectra	1373:1398	the pure component spectra	1373:1398	Through the analysis of the pure component spectra, it was possible to assess the chemical and structural composition of the saccaridic matrix, confirming the data obtained by NMR.
31637462	4	80	from	present	568:574	arg1	literature					583:592	the literature	579:592	the literature on details of its architecture including the spatial distribution of the macromolecular components which might give hints on the way the biofilm scaffold is built up by bacteria	579:770	Due to the complexity of the matrix, few information is present in the literature on details of its architecture including the spatial distribution of the macromolecular components which might give hints on the way the biofilm scaffold is built up by bacteria.
31637462	10	81	from	estimation	1807:1816	arg1	direction					1852:1860	the vertical direction	1839:1860	the vertical direction	1839:1860	In addition, nanoFTIR and AFM investigations allowed the estimation of biofilm growth in the vertical direction and the morphological analysis of bacterial colonies at different time points and the evaluation of the chemical composition at the nanoscale.
31637462	7	82	theme	local	1301:1305	arg1	distribution					1307:1318	the local distribution	1297:1318	the local distribution of the components within it	1297:1346	IR imaging permitted to have clues on how the biofilm grows and spreads on surfaces, and the local distribution of the components within it.
31637462	10	83	theme	growth	1829:1834	arg1	estimation					1807:1816	the estimation	1803:1816	the estimation of biofilm growth in the vertical direction	1803:1860	In addition, nanoFTIR and AFM investigations allowed the estimation of biofilm growth in the vertical direction and the morphological analysis of bacterial colonies at different time points and the evaluation of the chemical composition at the nanoscale.
31637462	10	83	theme	growth	1829:1834	arg1	analysis					1884:1891	the morphological analysis	1866:1891	the morphological analysis of bacterial colonies	1866:1913	In addition, nanoFTIR and AFM investigations allowed the estimation of biofilm growth in the vertical direction and the morphological analysis of bacterial colonies at different time points and the evaluation of the chemical composition at the nanoscale.
31637462	3	84	theme	dangerous	490:498	arg1	infections					500:509	dangerous infections	490:509	dangerous infections	490:509	However, they also contribute to increase bacterial resistance against antimicrobials, an issue particularly relevant in dangerous infections.
31637462	3	85	from	infections	500:509	arg1	relevant					478:485	relevant	478:485	relevant	478:485	However, they also contribute to increase bacterial resistance against antimicrobials, an issue particularly relevant in dangerous infections.
31637462	10	86	from	growth	1829:1834	arg1	direction					1852:1860	the vertical direction	1839:1860	the vertical direction	1839:1860	In addition, nanoFTIR and AFM investigations allowed the estimation of biofilm growth in the vertical direction and the morphological analysis of bacterial colonies at different time points and the evaluation of the chemical composition at the nanoscale.
31637462	10	87	theme	bacterial	1896:1904	arg1	colonies					1906:1913	bacterial colonies	1896:1913	bacterial colonies	1896:1913	In addition, nanoFTIR and AFM investigations allowed the estimation of biofilm growth in the vertical direction and the morphological analysis of bacterial colonies at different time points and the evaluation of the chemical composition at the nanoscale.
31637462	7	88	theme	IR	1208:1209	arg1	imaging					1211:1217	IR imaging	1208:1217	IR imaging	1208:1217	IR imaging permitted to have clues on how the biofilm grows and spreads on surfaces, and the local distribution of the components within it.
31637462	1	89	theme	extracellular	247:259	arg1	DNA					261:263	extracellular DNA	247:263	extracellular DNA	247:263	Biofilms are communities of bacteria living embedded in a highly hydrated matrix composed of polysaccharides, proteins, and extracellular DNA.
29867803	14	0	from	case	1980:1983	arg1	volunteers					1988:1997	volunteers	1988:1997	volunteers with greater adherence to the MD compared to those with lower adherence	1988:2069	This was also the case in volunteers with greater adherence to the MD compared to those with lower adherence.
29867803	7	1	theme	Dietary	1092:1098	arg1	information					1100:1110	Dietary information	1092:1110	Dietary information from healthy volunteers (n = 27)	1092:1143	Dietary information from healthy volunteers (n = 27) was recorded using the Food Frequency Questionnaire.
29867803	10	2	theme	metabolic	1398:1406	arg1	activity					1408:1415	Microbial metabolic activity	1388:1415	Microbial metabolic activity	1388:1415	Microbial metabolic activity was determined by quantification of short chain fatty acids (SCFA) on high performance liquid chromatography (HPLC).
29867803	2	3	theme	gut	312:314	arg1	populations					326:336	gut microbial populations	312:336	gut microbial populations	312:336	It is known that dysbiosis is related to disease and that most of the times this imbalances in gut microbial populations can be promoted through diet.
29867803	9	4	theme	Microbiota	1256:1265	arg1	composition					1267:1277	Microbiota composition	1256:1277	Microbiota composition	1256:1277	Microbiota composition and diversity were obtained by 16S rRNA gene sequencing and specific quantitative polymerase chain reaction.
29867803	16	5	theme	nutrients	2321:2329	arg1	consumption					2294:2304	greater consumption	2286:2304	greater consumption of plant-based nutrients, such as vegetable proteins and polysaccharides	2286:2377	Higher bifidobacterial counts, and higher total SCFA were related to greater consumption of plant-based nutrients, such as vegetable proteins and polysaccharides.
29867803	2	6	from	times	287:291	arg1	populations					326:336	gut microbial populations	312:336	gut microbial populations	312:336	It is known that dysbiosis is related to disease and that most of the times this imbalances in gut microbial populations can be promoted through diet.
29867803	16	7	theme	greater	2286:2292	arg1	consumption					2294:2304	greater consumption	2286:2304	greater consumption of plant-based nutrients, such as vegetable proteins and polysaccharides	2286:2377	Higher bifidobacterial counts, and higher total SCFA were related to greater consumption of plant-based nutrients, such as vegetable proteins and polysaccharides.
29867803	18	8	theme	specific	2493:2500	arg1	components					2510:2519	specific dietary components	2493:2519	specific dietary components	2493:2519	Consequently, diet and specific dietary components could affect microbiota composition, diversity, and activity, which may have an effect on host metabolism by increasing the risk of Western diseases.
29867803	16	9	theme	Higher	2217:2222	arg1	counts					2240:2245	Higher bifidobacterial counts	2217:2245	Higher bifidobacterial counts	2217:2245	Higher bifidobacterial counts, and higher total SCFA were related to greater consumption of plant-based nutrients, such as vegetable proteins and polysaccharides.
29867803	3	10	theme	simple	482:487	arg1	sugars					489:494	simple sugars	482:494	simple sugars	482:494	Western dietary habits, which are characterized by high intakes of calories, animal proteins, saturated fats, and simple sugars have been linked with higher risk of obesity, diabetes, cancer, and cardiovascular disease.
29867803	16	11	theme	vegetable	2340:2348	arg1	proteins					2350:2357	vegetable proteins	2340:2357	vegetable proteins	2340:2357	Higher bifidobacterial counts, and higher total SCFA were related to greater consumption of plant-based nutrients, such as vegetable proteins and polysaccharides.
29867803	6	12	theme	nutritional	1021:1031	arg1	habits					1033:1038	nutritional habits	1021:1038	nutritional habits as well as risk of disease related to these habits	1021:1089	Consequently, gut microbiota composition in healthy individuals, may be used as a potential biomarker to identify nutritional habits as well as risk of disease related to these habits.
29867803	10	13	theme	acids	1471:1475	arg1	quantification					1435:1448	quantification	1435:1448	quantification of short chain fatty acids (SCFA)	1435:1482	Microbial metabolic activity was determined by quantification of short chain fatty acids (SCFA) on high performance liquid chromatography (HPLC).
29867803	11	14	theme	greater	1650:1656	arg1	presence					1658:1665	greater presence	1650:1665	greater presence of Bacteroidetes	1650:1682	The results indicated that a higher ratio of Firmicutes-Bacteroidetes was related to lower adherence to the MD, and greater presence of Bacteroidetes was associated with lower animal protein intake.
29867803	3	15	theme	higher	518:523	arg1	risk					525:528	higher risk	518:528	higher risk of obesity, diabetes, cancer, and cardiovascular disease	518:585	Western dietary habits, which are characterized by high intakes of calories, animal proteins, saturated fats, and simple sugars have been linked with higher risk of obesity, diabetes, cancer, and cardiovascular disease.
29867803	5	16	theme	nutrient	773:780	arg1	compounds					782:790	nutrient compounds	773:790	nutrient compounds as well as adherence to a dietary pattern, as the Mediterranean diet (MD)	773:864	The aim of our study is to determine the effect of nutrient compounds as well as adherence to a dietary pattern, as the Mediterranean diet (MD) on the gut microbiome of healthy adults.
29867803	4	17	theme	dietary	651:657	arg1	components					659:668	dietary components	651:668	dietary components	651:668	However, little is known about the impact of dietary patterns, dietary components, and nutrients on gut microbiota in healthy people.
29867803	3	18	theme	saturated	462:470	arg1	fats					472:475	saturated fats	462:475	saturated fats	462:475	Western dietary habits, which are characterized by high intakes of calories, animal proteins, saturated fats, and simple sugars have been linked with higher risk of obesity, diabetes, cancer, and cardiovascular disease.
29867803	9	19	theme	rRNA	1314:1317	arg1	sequencing					1324:1333	rRNA gene sequencing	1314:1333	rRNA gene sequencing	1314:1333	Microbiota composition and diversity were obtained by 16S rRNA gene sequencing and specific quantitative polymerase chain reaction.
29867803	15	20	theme	genus	2165:2169	arg1	Catenibacterium					2171:2185	the genus Catenibacterium	2161:2185	the genus Catenibacterium	2161:2185	Butyricimonas, Desulfovibrio, and Oscillospira genera were associated with a BMI <25 and the genus Catenibacterium with a higher PREDIMED score.
29867803	12	21	theme	microbiota	1809:1818	arg1	diversity					1820:1828	gut microbiota diversity	1805:1828	gut microbiota diversity	1805:1828	High consumption of animal protein, saturated fats, and sugars affected gut microbiota diversity.
29867803	18	22	theme	dietary	2502:2508	arg1	components					2510:2519	specific dietary components	2493:2519	specific dietary components	2493:2519	Consequently, diet and specific dietary components could affect microbiota composition, diversity, and activity, which may have an effect on host metabolism by increasing the risk of Western diseases.
29867803	4	23	theme	dietary	633:639	arg1	patterns					641:648	dietary patterns	633:648	dietary patterns	633:648	However, little is known about the impact of dietary patterns, dietary components, and nutrients on gut microbiota in healthy people.
29867803	14	24	theme	greater	2004:2010	arg1	adherence					2012:2020	greater adherence	2004:2020	greater adherence to the MD compared to those with lower adherence	2004:2069	This was also the case in volunteers with greater adherence to the MD compared to those with lower adherence.
29867803	9	25	theme	specific	1339:1346	arg1	reaction					1378:1385	specific quantitative polymerase chain reaction	1339:1385	specific quantitative polymerase chain reaction	1339:1385	Microbiota composition and diversity were obtained by 16S rRNA gene sequencing and specific quantitative polymerase chain reaction.
29867803	6	26	theme	related	1067:1073	arg1	habits					1033:1038	nutritional habits	1021:1038	nutritional habits as well as risk of disease related to these habits	1021:1089	Consequently, gut microbiota composition in healthy individuals, may be used as a potential biomarker to identify nutritional habits as well as risk of disease related to these habits.
29867803	15	27	theme	PREDIMED	2201:2208	arg1	score					2210:2214	a higher PREDIMED score	2192:2214	a higher PREDIMED score	2192:2214	Butyricimonas, Desulfovibrio, and Oscillospira genera were associated with a BMI <25 and the genus Catenibacterium with a higher PREDIMED score.
29867803	6	28	used	used	979:982	arg2	biomarker					999:1007	a potential biomarker	987:1007	a potential biomarker to identify nutritional habits as well as risk of disease related to these habits	987:1089	Consequently, gut microbiota composition in healthy individuals, may be used as a potential biomarker to identify nutritional habits as well as risk of disease related to these habits.
29867803	6	28	used	used	979:982	arg2	composition					936:946	gut microbiota composition	921:946	gut microbiota composition	921:946	Consequently, gut microbiota composition in healthy individuals, may be used as a potential biomarker to identify nutritional habits as well as risk of disease related to these habits.
29867803	9	29	theme	polymerase	1361:1370	arg1	reaction					1378:1385	specific quantitative polymerase chain reaction	1339:1385	specific quantitative polymerase chain reaction	1339:1385	Microbiota composition and diversity were obtained by 16S rRNA gene sequencing and specific quantitative polymerase chain reaction.
29867803	0	30	from	Intakes	89:95	arg1	Population					114:123	General Adult Population	100:123	General Adult Population	100:123	Shifts on Gut Microbiota Associated to Mediterranean Diet Adherence and Specific Dietary Intakes on General Adult Population.
29867803	0	31	theme	Specific	72:79	arg1	Intakes					89:95	Specific Dietary Intakes	72:95	Specific Dietary Intakes	72:95	Shifts on Gut Microbiota Associated to Mediterranean Diet Adherence and Specific Dietary Intakes on General Adult Population.
29867803	12	32	theme	saturated	1769:1777	arg1	fats					1779:1782	saturated fats	1769:1782	saturated fats	1769:1782	High consumption of animal protein, saturated fats, and sugars affected gut microbiota diversity.
29867803	4	33	from	microbiota	692:701	arg1	people					714:719	healthy people	706:719	healthy people	706:719	However, little is known about the impact of dietary patterns, dietary components, and nutrients on gut microbiota in healthy people.
29867803	0	34	from	Shifts	0:5	arg1	Microbiota					14:23	Gut Microbiota	10:23	Gut Microbiota	10:23	Shifts on Gut Microbiota Associated to Mediterranean Diet Adherence and Specific Dietary Intakes on General Adult Population.
29867803	18	35	theme	host	2611:2614	arg1	metabolism					2616:2625	host metabolism	2611:2625	host metabolism	2611:2625	Consequently, diet and specific dietary components could affect microbiota composition, diversity, and activity, which may have an effect on host metabolism by increasing the risk of Western diseases.
29867803	5	36	theme	dietary	818:824	arg1	pattern					826:832	a dietary pattern	816:832	a dietary pattern	816:832	The aim of our study is to determine the effect of nutrient compounds as well as adherence to a dietary pattern, as the Mediterranean diet (MD) on the gut microbiome of healthy adults.
29867803	0	37	theme	General	100:106	arg1	Population					114:123	General Adult Population	100:123	General Adult Population	100:123	Shifts on Gut Microbiota Associated to Mediterranean Diet Adherence and Specific Dietary Intakes on General Adult Population.
29867803	7	38	theme	Frequency	1173:1181	arg1	Questionnaire					1183:1195	the Food Frequency Questionnaire	1164:1195	the Food Frequency Questionnaire	1164:1195	Dietary information from healthy volunteers (n = 27) was recorded using the Food Frequency Questionnaire.
29867803	4	39	theme	components	659:668	arg1	impact					623:628	the impact	619:628	the impact of dietary patterns, dietary components, and nutrients on gut microbiota in healthy people	619:719	However, little is known about the impact of dietary patterns, dietary components, and nutrients on gut microbiota in healthy people.
29867803	10	40	theme	liquid	1504:1509	arg1	chromatography					1511:1524	high performance liquid chromatography	1487:1524	high performance liquid chromatography (HPLC)	1487:1531	Microbial metabolic activity was determined by quantification of short chain fatty acids (SCFA) on high performance liquid chromatography (HPLC).
29867803	10	40	theme	liquid	1504:1509	arg1	HPLC					1527:1530	HPLC	1527:1530	HPLC	1527:1530	Microbial metabolic activity was determined by quantification of short chain fatty acids (SCFA) on high performance liquid chromatography (HPLC).
29867803	12	41	theme	protein	1760:1766	arg1	consumption					1738:1748	High consumption	1733:1748	High consumption of animal protein, saturated fats, and sugars	1733:1794	High consumption of animal protein, saturated fats, and sugars affected gut microbiota diversity.
29867803	6	42	theme	microbiota	925:934	arg1	composition					936:946	gut microbiota composition	921:946	gut microbiota composition	921:946	Consequently, gut microbiota composition in healthy individuals, may be used as a potential biomarker to identify nutritional habits as well as risk of disease related to these habits.
29867803	6	42	theme	microbiota	925:934	arg1	biomarker					999:1007	a potential biomarker	987:1007	a potential biomarker to identify nutritional habits as well as risk of disease related to these habits	987:1089	Consequently, gut microbiota composition in healthy individuals, may be used as a potential biomarker to identify nutritional habits as well as risk of disease related to these habits.
29867803	17	43	theme	total	2458:2462	arg1	SCFA					2464:2467	total SCFA	2458:2467	total SCFA	2458:2467	Better adherence to the MD was associated with significantly higher levels of total SCFA.
29867803	0	44	theme	Gut	10:12	arg1	Microbiota					14:23	Gut Microbiota	10:23	Gut Microbiota	10:23	Shifts on Gut Microbiota Associated to Mediterranean Diet Adherence and Specific Dietary Intakes on General Adult Population.
29867803	9	45	theme	chain	1372:1376	arg1	reaction					1378:1385	specific quantitative polymerase chain reaction	1339:1385	specific quantitative polymerase chain reaction	1339:1385	Microbiota composition and diversity were obtained by 16S rRNA gene sequencing and specific quantitative polymerase chain reaction.
29867803	3	46	theme	dietary	376:382	arg1	habits					384:389	Western dietary habits	368:389	Western dietary habits	368:389	Western dietary habits, which are characterized by high intakes of calories, animal proteins, saturated fats, and simple sugars have been linked with higher risk of obesity, diabetes, cancer, and cardiovascular disease.
29867803	12	47	theme	High	1733:1736	arg1	consumption					1738:1748	High consumption	1733:1748	High consumption of animal protein, saturated fats, and sugars	1733:1794	High consumption of animal protein, saturated fats, and sugars affected gut microbiota diversity.
29867803	10	48	theme	Microbial	1388:1396	arg1	activity					1408:1415	Microbial metabolic activity	1388:1415	Microbial metabolic activity	1388:1415	Microbial metabolic activity was determined by quantification of short chain fatty acids (SCFA) on high performance liquid chromatography (HPLC).
29867803	3	49	theme	cardiovascular	564:577	arg1	disease					579:585	cardiovascular disease	564:585	cardiovascular disease	564:585	Western dietary habits, which are characterized by high intakes of calories, animal proteins, saturated fats, and simple sugars have been linked with higher risk of obesity, diabetes, cancer, and cardiovascular disease.
29867803	16	50	theme	higher	2252:2257	arg1	SCFA					2265:2268	higher total SCFA	2252:2268	higher total SCFA	2252:2268	Higher bifidobacterial counts, and higher total SCFA were related to greater consumption of plant-based nutrients, such as vegetable proteins and polysaccharides.
29867803	3	51	theme	animal	445:450	arg1	proteins					452:459	animal proteins	445:459	animal proteins	445:459	Western dietary habits, which are characterized by high intakes of calories, animal proteins, saturated fats, and simple sugars have been linked with higher risk of obesity, diabetes, cancer, and cardiovascular disease.
29867803	0	52	theme	Mediterranean	39:51	arg1	Diet					53:56	Mediterranean Diet Adherence and Specific Dietary Intakes	39:95	Diet	53:56	Shifts on Gut Microbiota Associated to Mediterranean Diet Adherence and Specific Dietary Intakes on General Adult Population.
29867803	5	53	theme	adults	899:904	arg1	microbiome					877:886	the gut microbiome	869:886	the gut microbiome of healthy adults	869:904	The aim of our study is to determine the effect of nutrient compounds as well as adherence to a dietary pattern, as the Mediterranean diet (MD) on the gut microbiome of healthy adults.
29867803	7	54	theme	=	1139:1139	arg1	n					1137:1137	n = 27	1137:1142	n = 27	1137:1142	Dietary information from healthy volunteers (n = 27) was recorded using the Food Frequency Questionnaire.
29867803	7	54	theme	=	1139:1139	arg1	volunteers					1125:1134	healthy volunteers	1117:1134	healthy volunteers (n = 27)	1117:1143	Dietary information from healthy volunteers (n = 27) was recorded using the Food Frequency Questionnaire.
29867803	11	55	theme	Firmicutes-Bacteroidetes	1579:1602	arg1	ratio					1570:1574	a higher ratio	1561:1574	a higher ratio of Firmicutes-Bacteroidetes	1561:1602	The results indicated that a higher ratio of Firmicutes-Bacteroidetes was related to lower adherence to the MD, and greater presence of Bacteroidetes was associated with lower animal protein intake.
29867803	11	55	theme	Firmicutes-Bacteroidetes	1579:1602	arg1	related					1608:1614	related	1608:1614	related	1608:1614	The results indicated that a higher ratio of Firmicutes-Bacteroidetes was related to lower adherence to the MD, and greater presence of Bacteroidetes was associated with lower animal protein intake.
29867803	4	56	theme	healthy	706:712	arg1	people					714:719	healthy people	706:719	healthy people	706:719	However, little is known about the impact of dietary patterns, dietary components, and nutrients on gut microbiota in healthy people.
29867803	13	57	theme	normal-weight	1897:1909	arg1	individuals					1911:1921	normal-weight individuals	1897:1921	normal-weight individuals	1897:1921	A significant higher presence of Christensenellaceae was found in normal-weight individuals compared to those who were overweight.
29867803	3	58	theme	calories	435:442	arg1	intakes					424:430	high intakes	419:430	high intakes of calories, animal proteins, saturated fats, and simple sugars	419:494	Western dietary habits, which are characterized by high intakes of calories, animal proteins, saturated fats, and simple sugars have been linked with higher risk of obesity, diabetes, cancer, and cardiovascular disease.
29867803	3	59	theme	fats	472:475	arg1	intakes					424:430	high intakes	419:430	high intakes of calories, animal proteins, saturated fats, and simple sugars	419:494	Western dietary habits, which are characterized by high intakes of calories, animal proteins, saturated fats, and simple sugars have been linked with higher risk of obesity, diabetes, cancer, and cardiovascular disease.
29867803	16	60	theme	plant-based	2309:2319	arg1	nutrients					2321:2329	plant-based nutrients	2309:2329	plant-based nutrients	2309:2329	Higher bifidobacterial counts, and higher total SCFA were related to greater consumption of plant-based nutrients, such as vegetable proteins and polysaccharides.
29867803	16	60	theme	plant-based	2309:2319	arg1	polysaccharides					2363:2377	polysaccharides	2363:2377	polysaccharides	2363:2377	Higher bifidobacterial counts, and higher total SCFA were related to greater consumption of plant-based nutrients, such as vegetable proteins and polysaccharides.
29867803	16	60	theme	plant-based	2309:2319	arg1	proteins					2350:2357	vegetable proteins	2340:2357	vegetable proteins	2340:2357	Higher bifidobacterial counts, and higher total SCFA were related to greater consumption of plant-based nutrients, such as vegetable proteins and polysaccharides.
29867803	5	61	theme	gut	873:875	arg1	microbiome					877:886	the gut microbiome	869:886	the gut microbiome of healthy adults	869:904	The aim of our study is to determine the effect of nutrient compounds as well as adherence to a dietary pattern, as the Mediterranean diet (MD) on the gut microbiome of healthy adults.
29867803	7	62	theme	healthy	1117:1123	arg1	n					1137:1137	n = 27	1137:1142	n = 27	1137:1142	Dietary information from healthy volunteers (n = 27) was recorded using the Food Frequency Questionnaire.
29867803	7	62	theme	healthy	1117:1123	arg1	volunteers					1125:1134	healthy volunteers	1117:1134	healthy volunteers (n = 27)	1117:1143	Dietary information from healthy volunteers (n = 27) was recorded using the Food Frequency Questionnaire.
29867803	13	63	theme	Christensenellaceae	1864:1882	arg1	presence					1852:1859	A significant higher presence	1831:1859	A significant higher presence of Christensenellaceae	1831:1882	A significant higher presence of Christensenellaceae was found in normal-weight individuals compared to those who were overweight.
29867803	2	64	theme	microbial	316:324	arg1	populations					326:336	gut microbial populations	312:336	gut microbial populations	312:336	It is known that dysbiosis is related to disease and that most of the times this imbalances in gut microbial populations can be promoted through diet.
29867803	10	65	theme	short	1453:1457	arg1	acids					1471:1475	short chain fatty acids	1453:1475	short chain fatty acids (SCFA)	1453:1482	Microbial metabolic activity was determined by quantification of short chain fatty acids (SCFA) on high performance liquid chromatography (HPLC).
29867803	10	65	theme	short	1453:1457	arg1	SCFA					1478:1481	SCFA	1478:1481	SCFA	1478:1481	Microbial metabolic activity was determined by quantification of short chain fatty acids (SCFA) on high performance liquid chromatography (HPLC).
29867803	18	66	theme	Western	2653:2659	arg1	diseases					2661:2668	Western diseases	2653:2668	Western diseases	2653:2668	Consequently, diet and specific dietary components could affect microbiota composition, diversity, and activity, which may have an effect on host metabolism by increasing the risk of Western diseases.
29867803	3	67	theme	sugars	489:494	arg1	intakes					424:430	high intakes	419:430	high intakes of calories, animal proteins, saturated fats, and simple sugars	419:494	Western dietary habits, which are characterized by high intakes of calories, animal proteins, saturated fats, and simple sugars have been linked with higher risk of obesity, diabetes, cancer, and cardiovascular disease.
29867803	14	68	theme	lower	2055:2059	arg1	adherence					2061:2069	lower adherence	2055:2069	lower adherence	2055:2069	This was also the case in volunteers with greater adherence to the MD compared to those with lower adherence.
29867803	11	69	theme	animal	1710:1715	arg1	intake					1725:1730	lower animal protein intake	1704:1730	lower animal protein intake	1704:1730	The results indicated that a higher ratio of Firmicutes-Bacteroidetes was related to lower adherence to the MD, and greater presence of Bacteroidetes was associated with lower animal protein intake.
29867803	13	70	theme	significant	1833:1843	arg1	presence					1852:1859	A significant higher presence	1831:1859	A significant higher presence of Christensenellaceae	1831:1882	A significant higher presence of Christensenellaceae was found in normal-weight individuals compared to those who were overweight.
29867803	7	71	from	volunteers	1125:1134	arg1	information					1100:1110	Dietary information	1092:1110	Dietary information from healthy volunteers (n = 27)	1092:1143	Dietary information from healthy volunteers (n = 27) was recorded using the Food Frequency Questionnaire.
29867803	16	72	theme	bifidobacterial	2224:2238	arg1	counts					2240:2245	Higher bifidobacterial counts	2217:2245	Higher bifidobacterial counts	2217:2245	Higher bifidobacterial counts, and higher total SCFA were related to greater consumption of plant-based nutrients, such as vegetable proteins and polysaccharides.
29867803	1	73	theme	gut	183:185	arg1	microbiome					187:196	gut microbiome	183:196	gut microbiome	183:196	There is increasing evidence for the interaction between gut microbiome, diet, and health.
29867803	13	74	located	found	1888:1892	arg1	individuals					1911:1921	normal-weight individuals	1897:1921	normal-weight individuals	1897:1921	A significant higher presence of Christensenellaceae was found in normal-weight individuals compared to those who were overweight.
29867803	13	74	located	found	1888:1892	arg2	presence					1852:1859	A significant higher presence	1831:1859	A significant higher presence of Christensenellaceae	1831:1882	A significant higher presence of Christensenellaceae was found in normal-weight individuals compared to those who were overweight.
29867803	10	75	theme	fatty	1465:1469	arg1	acids					1471:1475	short chain fatty acids	1453:1475	short chain fatty acids (SCFA)	1453:1482	Microbial metabolic activity was determined by quantification of short chain fatty acids (SCFA) on high performance liquid chromatography (HPLC).
29867803	10	75	theme	fatty	1465:1469	arg1	SCFA					1478:1481	SCFA	1478:1481	SCFA	1478:1481	Microbial metabolic activity was determined by quantification of short chain fatty acids (SCFA) on high performance liquid chromatography (HPLC).
29867803	5	76	theme	study	737:741	arg1	aim					726:728	The aim	722:728	The aim of our study	722:741	The aim of our study is to determine the effect of nutrient compounds as well as adherence to a dietary pattern, as the Mediterranean diet (MD) on the gut microbiome of healthy adults.
29867803	1	77	theme	increasing	135:144	arg1	evidence					146:153	increasing evidence	135:153	increasing evidence for the interaction between gut microbiome, diet, and health	135:214	There is increasing evidence for the interaction between gut microbiome, diet, and health.
29867803	10	78	theme	high	1487:1490	arg1	chromatography					1511:1524	high performance liquid chromatography	1487:1524	high performance liquid chromatography (HPLC)	1487:1531	Microbial metabolic activity was determined by quantification of short chain fatty acids (SCFA) on high performance liquid chromatography (HPLC).
29867803	10	78	theme	high	1487:1490	arg1	HPLC					1527:1530	HPLC	1527:1530	HPLC	1527:1530	Microbial metabolic activity was determined by quantification of short chain fatty acids (SCFA) on high performance liquid chromatography (HPLC).
29867803	3	79	theme	proteins	452:459	arg1	intakes					424:430	high intakes	419:430	high intakes of calories, animal proteins, saturated fats, and simple sugars	419:494	Western dietary habits, which are characterized by high intakes of calories, animal proteins, saturated fats, and simple sugars have been linked with higher risk of obesity, diabetes, cancer, and cardiovascular disease.
29867803	3	80	theme	obesity	533:539	arg1	risk					525:528	higher risk	518:528	higher risk of obesity, diabetes, cancer, and cardiovascular disease	518:585	Western dietary habits, which are characterized by high intakes of calories, animal proteins, saturated fats, and simple sugars have been linked with higher risk of obesity, diabetes, cancer, and cardiovascular disease.
29867803	9	81	theme	gene	1319:1322	arg1	sequencing					1324:1333	rRNA gene sequencing	1314:1333	rRNA gene sequencing	1314:1333	Microbiota composition and diversity were obtained by 16S rRNA gene sequencing and specific quantitative polymerase chain reaction.
29867803	12	82	theme	sugars	1789:1794	arg1	consumption					1738:1748	High consumption	1733:1748	High consumption of animal protein, saturated fats, and sugars	1733:1794	High consumption of animal protein, saturated fats, and sugars affected gut microbiota diversity.
29867803	17	83	theme	SCFA	2464:2467	arg1	levels					2448:2453	significantly higher levels	2427:2453	significantly higher levels of total SCFA	2427:2467	Better adherence to the MD was associated with significantly higher levels of total SCFA.
29867803	5	84	theme	adherence	803:811	arg1	effect					763:768	the effect	759:768	the effect of nutrient compounds as well as adherence to a dietary pattern, as the Mediterranean diet (MD) on the gut microbiome of healthy adults	759:904	The aim of our study is to determine the effect of nutrient compounds as well as adherence to a dietary pattern, as the Mediterranean diet (MD) on the gut microbiome of healthy adults.
29867803	12	85	theme	gut	1805:1807	arg1	diversity					1820:1828	gut microbiota diversity	1805:1828	gut microbiota diversity	1805:1828	High consumption of animal protein, saturated fats, and sugars affected gut microbiota diversity.
29867803	11	86	theme	Bacteroidetes	1670:1682	arg1	presence					1658:1665	greater presence	1650:1665	greater presence of Bacteroidetes	1650:1682	The results indicated that a higher ratio of Firmicutes-Bacteroidetes was related to lower adherence to the MD, and greater presence of Bacteroidetes was associated with lower animal protein intake.
29867803	3	87	theme	cancer	552:557	arg1	risk					525:528	higher risk	518:528	higher risk of obesity, diabetes, cancer, and cardiovascular disease	518:585	Western dietary habits, which are characterized by high intakes of calories, animal proteins, saturated fats, and simple sugars have been linked with higher risk of obesity, diabetes, cancer, and cardiovascular disease.
29867803	9	88	theme	quantitative	1348:1359	arg1	reaction					1378:1385	specific quantitative polymerase chain reaction	1339:1385	specific quantitative polymerase chain reaction	1339:1385	Microbiota composition and diversity were obtained by 16S rRNA gene sequencing and specific quantitative polymerase chain reaction.
29867803	13	89	dep	significant	1833:1843	arg1	higher					1845:1850	higher	1845:1850	higher	1845:1850	A significant higher presence of Christensenellaceae was found in normal-weight individuals compared to those who were overweight.
29867803	14	90	with	volunteers	1988:1997	arg1	adherence					2012:2020	greater adherence	2004:2020	greater adherence to the MD compared to those with lower adherence	2004:2069	This was also the case in volunteers with greater adherence to the MD compared to those with lower adherence.
29867803	6	91	theme	disease	1059:1065	arg1	risk					1051:1054	risk	1051:1054	nutritional habits as well as risk of disease related to these habits	1021:1089	Consequently, gut microbiota composition in healthy individuals, may be used as a potential biomarker to identify nutritional habits as well as risk of disease related to these habits.
29867803	6	91	theme	disease	1059:1065	arg1	habits					1033:1038	nutritional habits	1021:1038	nutritional habits as well as risk of disease related to these habits	1021:1089	Consequently, gut microbiota composition in healthy individuals, may be used as a potential biomarker to identify nutritional habits as well as risk of disease related to these habits.
29867803	4	92	theme	patterns	641:648	arg1	impact					623:628	the impact	619:628	the impact of dietary patterns, dietary components, and nutrients on gut microbiota in healthy people	619:719	However, little is known about the impact of dietary patterns, dietary components, and nutrients on gut microbiota in healthy people.
29867803	11	93	theme	lower	1619:1623	arg1	adherence					1625:1633	lower adherence	1619:1633	lower adherence to the MD	1619:1643	The results indicated that a higher ratio of Firmicutes-Bacteroidetes was related to lower adherence to the MD, and greater presence of Bacteroidetes was associated with lower animal protein intake.
29867803	3	94	theme	diabetes	542:549	arg1	risk					525:528	higher risk	518:528	higher risk of obesity, diabetes, cancer, and cardiovascular disease	518:585	Western dietary habits, which are characterized by high intakes of calories, animal proteins, saturated fats, and simple sugars have been linked with higher risk of obesity, diabetes, cancer, and cardiovascular disease.
29867803	5	95	theme	compounds	782:790	arg1	effect					763:768	the effect	759:768	the effect of nutrient compounds as well as adherence to a dietary pattern, as the Mediterranean diet (MD) on the gut microbiome of healthy adults	759:904	The aim of our study is to determine the effect of nutrient compounds as well as adherence to a dietary pattern, as the Mediterranean diet (MD) on the gut microbiome of healthy adults.
29867803	10	96	theme	chain	1459:1463	arg1	acids					1471:1475	short chain fatty acids	1453:1475	short chain fatty acids (SCFA)	1453:1482	Microbial metabolic activity was determined by quantification of short chain fatty acids (SCFA) on high performance liquid chromatography (HPLC).
29867803	10	96	theme	chain	1459:1463	arg1	SCFA					1478:1481	SCFA	1478:1481	SCFA	1478:1481	Microbial metabolic activity was determined by quantification of short chain fatty acids (SCFA) on high performance liquid chromatography (HPLC).
29867803	0	97	theme	Dietary	81:87	arg1	Intakes					89:95	Specific Dietary Intakes	72:95	Specific Dietary Intakes	72:95	Shifts on Gut Microbiota Associated to Mediterranean Diet Adherence and Specific Dietary Intakes on General Adult Population.
29867803	12	98	theme	fats	1779:1782	arg1	consumption					1738:1748	High consumption	1733:1748	High consumption of animal protein, saturated fats, and sugars	1733:1794	High consumption of animal protein, saturated fats, and sugars affected gut microbiota diversity.
29867803	5	99	from	effect	763:768	arg1	microbiome					877:886	the gut microbiome	869:886	the gut microbiome of healthy adults	869:904	The aim of our study is to determine the effect of nutrient compounds as well as adherence to a dietary pattern, as the Mediterranean diet (MD) on the gut microbiome of healthy adults.
29867803	5	100	theme	Mediterranean	842:854	arg1	diet					856:859	the Mediterranean diet	838:859	the Mediterranean diet (MD)	838:864	The aim of our study is to determine the effect of nutrient compounds as well as adherence to a dietary pattern, as the Mediterranean diet (MD) on the gut microbiome of healthy adults.
29867803	5	100	theme	Mediterranean	842:854	arg1	MD					862:863	MD	862:863	MD	862:863	The aim of our study is to determine the effect of nutrient compounds as well as adherence to a dietary pattern, as the Mediterranean diet (MD) on the gut microbiome of healthy adults.
29867803	0	101	from	Diet	53:56	arg1	Population					114:123	General Adult Population	100:123	General Adult Population	100:123	Shifts on Gut Microbiota Associated to Mediterranean Diet Adherence and Specific Dietary Intakes on General Adult Population.
29867803	4	102	theme	nutrients	675:683	arg1	impact					623:628	the impact	619:628	the impact of dietary patterns, dietary components, and nutrients on gut microbiota in healthy people	619:719	However, little is known about the impact of dietary patterns, dietary components, and nutrients on gut microbiota in healthy people.
29867803	18	103	theme	microbiota	2534:2543	arg1	composition					2545:2555	microbiota composition	2534:2555	microbiota composition	2534:2555	Consequently, diet and specific dietary components could affect microbiota composition, diversity, and activity, which may have an effect on host metabolism by increasing the risk of Western diseases.
29867803	0	104	theme	Adult	108:112	arg1	Population					114:123	General Adult Population	100:123	General Adult Population	100:123	Shifts on Gut Microbiota Associated to Mediterranean Diet Adherence and Specific Dietary Intakes on General Adult Population.
29867803	12	105	theme	animal	1753:1758	arg1	protein					1760:1766	animal protein	1753:1766	animal protein	1753:1766	High consumption of animal protein, saturated fats, and sugars affected gut microbiota diversity.
29867803	6	106	theme	healthy	951:957	arg1	individuals					959:969	healthy individuals	951:969	healthy individuals	951:969	Consequently, gut microbiota composition in healthy individuals, may be used as a potential biomarker to identify nutritional habits as well as risk of disease related to these habits.
29867803	18	107	contain	have	2593:2596	arg2	effect					2601:2606	an effect	2598:2606	an effect on host metabolism	2598:2625	Consequently, diet and specific dietary components could affect microbiota composition, diversity, and activity, which may have an effect on host metabolism by increasing the risk of Western diseases.
29867803	18	107	contain	have	2593:2596	arg1	diversity					2558:2566	diversity	2558:2566	diversity	2558:2566	Consequently, diet and specific dietary components could affect microbiota composition, diversity, and activity, which may have an effect on host metabolism by increasing the risk of Western diseases.
29867803	18	107	contain	have	2593:2596	arg1	activity					2573:2580	activity	2573:2580	activity	2573:2580	Consequently, diet and specific dietary components could affect microbiota composition, diversity, and activity, which may have an effect on host metabolism by increasing the risk of Western diseases.
29867803	18	107	contain	have	2593:2596	arg1	composition					2545:2555	microbiota composition	2534:2555	microbiota composition	2534:2555	Consequently, diet and specific dietary components could affect microbiota composition, diversity, and activity, which may have an effect on host metabolism by increasing the risk of Western diseases.
29867803	17	108	theme	higher	2441:2446	arg1	levels					2448:2453	significantly higher levels	2427:2453	significantly higher levels of total SCFA	2427:2467	Better adherence to the MD was associated with significantly higher levels of total SCFA.
29867803	4	109	theme	gut	688:690	arg1	microbiota					692:701	gut microbiota	688:701	gut microbiota in healthy people	688:719	However, little is known about the impact of dietary patterns, dietary components, and nutrients on gut microbiota in healthy people.
29867803	2	110	dep	times	287:291	arg1	imbalances					298:307	this imbalances	293:307	the times this imbalances in gut microbial populations	283:336	It is known that dysbiosis is related to disease and that most of the times this imbalances in gut microbial populations can be promoted through diet.
29867803	10	111	theme	performance	1492:1502	arg1	chromatography					1511:1524	high performance liquid chromatography	1487:1524	high performance liquid chromatography (HPLC)	1487:1531	Microbial metabolic activity was determined by quantification of short chain fatty acids (SCFA) on high performance liquid chromatography (HPLC).
29867803	10	111	theme	performance	1492:1502	arg1	HPLC					1527:1530	HPLC	1527:1530	HPLC	1527:1530	Microbial metabolic activity was determined by quantification of short chain fatty acids (SCFA) on high performance liquid chromatography (HPLC).
29867803	18	112	from	effect	2601:2606	arg1	metabolism					2616:2625	host metabolism	2611:2625	host metabolism	2611:2625	Consequently, diet and specific dietary components could affect microbiota composition, diversity, and activity, which may have an effect on host metabolism by increasing the risk of Western diseases.
29867803	6	113	theme	gut	921:923	arg1	composition					936:946	gut microbiota composition	921:946	gut microbiota composition	921:946	Consequently, gut microbiota composition in healthy individuals, may be used as a potential biomarker to identify nutritional habits as well as risk of disease related to these habits.
29867803	6	113	theme	gut	921:923	arg1	biomarker					999:1007	a potential biomarker	987:1007	a potential biomarker to identify nutritional habits as well as risk of disease related to these habits	987:1089	Consequently, gut microbiota composition in healthy individuals, may be used as a potential biomarker to identify nutritional habits as well as risk of disease related to these habits.
29867803	3	114	theme	disease	579:585	arg1	risk					525:528	higher risk	518:528	higher risk of obesity, diabetes, cancer, and cardiovascular disease	518:585	Western dietary habits, which are characterized by high intakes of calories, animal proteins, saturated fats, and simple sugars have been linked with higher risk of obesity, diabetes, cancer, and cardiovascular disease.
29867803	0	115	dep	Diet	53:56	arg1	Adherence					58:66	Adherence	58:66	Adherence	58:66	Shifts on Gut Microbiota Associated to Mediterranean Diet Adherence and Specific Dietary Intakes on General Adult Population.
29867803	7	116	theme	Food	1168:1171	arg1	Questionnaire					1183:1195	the Food Frequency Questionnaire	1164:1195	the Food Frequency Questionnaire	1164:1195	Dietary information from healthy volunteers (n = 27) was recorded using the Food Frequency Questionnaire.
29867803	16	117	theme	total	2259:2263	arg1	SCFA					2265:2268	higher total SCFA	2252:2268	higher total SCFA	2252:2268	Higher bifidobacterial counts, and higher total SCFA were related to greater consumption of plant-based nutrients, such as vegetable proteins and polysaccharides.
29867803	3	118	theme	high	419:422	arg1	intakes					424:430	high intakes	419:430	high intakes of calories, animal proteins, saturated fats, and simple sugars	419:494	Western dietary habits, which are characterized by high intakes of calories, animal proteins, saturated fats, and simple sugars have been linked with higher risk of obesity, diabetes, cancer, and cardiovascular disease.
29867803	5	119	theme	healthy	891:897	arg1	adults					899:904	healthy adults	891:904	healthy adults	891:904	The aim of our study is to determine the effect of nutrient compounds as well as adherence to a dietary pattern, as the Mediterranean diet (MD) on the gut microbiome of healthy adults.
29867803	6	120	theme	potential	989:997	arg1	composition					936:946	gut microbiota composition	921:946	gut microbiota composition	921:946	Consequently, gut microbiota composition in healthy individuals, may be used as a potential biomarker to identify nutritional habits as well as risk of disease related to these habits.
29867803	6	120	theme	potential	989:997	arg1	biomarker					999:1007	a potential biomarker	987:1007	a potential biomarker to identify nutritional habits as well as risk of disease related to these habits	987:1089	Consequently, gut microbiota composition in healthy individuals, may be used as a potential biomarker to identify nutritional habits as well as risk of disease related to these habits.
29867803	3	121	theme	Western	368:374	arg1	habits					384:389	Western dietary habits	368:389	Western dietary habits	368:389	Western dietary habits, which are characterized by high intakes of calories, animal proteins, saturated fats, and simple sugars have been linked with higher risk of obesity, diabetes, cancer, and cardiovascular disease.
29867803	8	122	theme	PREDIMED	1241:1248	arg1	test					1250:1253	the PREDIMED test	1237:1253	the PREDIMED test	1237:1253	Adherence to the MD was measured using the PREDIMED test.
29867803	18	123	theme	diseases	2661:2668	arg1	risk					2645:2648	the risk	2641:2648	the risk of Western diseases	2641:2668	Consequently, diet and specific dietary components could affect microbiota composition, diversity, and activity, which may have an effect on host metabolism by increasing the risk of Western diseases.
29867803	11	124	theme	protein	1717:1723	arg1	intake					1725:1730	lower animal protein intake	1704:1730	lower animal protein intake	1704:1730	The results indicated that a higher ratio of Firmicutes-Bacteroidetes was related to lower adherence to the MD, and greater presence of Bacteroidetes was associated with lower animal protein intake.
29867803	15	125	theme	Oscillospira	2106:2117	arg1	genera					2119:2124	Oscillospira genera	2106:2124	Oscillospira genera	2106:2124	Butyricimonas, Desulfovibrio, and Oscillospira genera were associated with a BMI <25 and the genus Catenibacterium with a higher PREDIMED score.
29867803	11	126	theme	higher	1563:1568	arg1	ratio					1570:1574	a higher ratio	1561:1574	a higher ratio of Firmicutes-Bacteroidetes	1561:1602	The results indicated that a higher ratio of Firmicutes-Bacteroidetes was related to lower adherence to the MD, and greater presence of Bacteroidetes was associated with lower animal protein intake.
29867803	11	126	theme	higher	1563:1568	arg1	related					1608:1614	related	1608:1614	related	1608:1614	The results indicated that a higher ratio of Firmicutes-Bacteroidetes was related to lower adherence to the MD, and greater presence of Bacteroidetes was associated with lower animal protein intake.
29867803	11	127	theme	lower	1704:1708	arg1	intake					1725:1730	lower animal protein intake	1704:1730	lower animal protein intake	1704:1730	The results indicated that a higher ratio of Firmicutes-Bacteroidetes was related to lower adherence to the MD, and greater presence of Bacteroidetes was associated with lower animal protein intake.
29867803	15	128	theme	higher	2194:2199	arg1	score					2210:2214	a higher PREDIMED score	2192:2214	a higher PREDIMED score	2192:2214	Butyricimonas, Desulfovibrio, and Oscillospira genera were associated with a BMI <25 and the genus Catenibacterium with a higher PREDIMED score.
29867803	17	129	theme	Better	2380:2385	arg1	adherence					2387:2395	Better adherence	2380:2395	Better adherence to the MD	2380:2405	Better adherence to the MD was associated with significantly higher levels of total SCFA.
29867803	4	130	from	impact	623:628	arg1	microbiota					692:701	gut microbiota	688:701	gut microbiota in healthy people	688:719	However, little is known about the impact of dietary patterns, dietary components, and nutrients on gut microbiota in healthy people.
29792669	8	0	theme	myelogenous	1159:1169	arg1	leukemia					1171:1178	acute myelogenous leukemia	1153:1178	acute myelogenous leukemia cell lysate	1153:1190	Moreover, the developed platform was successfully applied to analyze glycopetides in acute myelogenous leukemia cell lysate and human serum samples.
29792669	6	1	theme	host-guest	732:741	arg1	strategy					767:774	a host-guest self-assembly synthetic strategy	730:774	a host-guest self-assembly synthetic strategy	730:774	A solid monolithic support was employed to immobilize gluCDMT by a host-guest self-assembly synthetic strategy, which did not occupy the surface hydrophilic sites.
29792669	7	2	theme	good	902:905	arg1	ability					907:913	good ability	902:913	good ability to capture glycopeptides (23 HRP glycopeptides and 28 IgG glycopeptides)	902:986	The gluCDMT-based monolith exhibited high binding capacity (∼50 mg g-1), good ability to capture glycopeptides (23 HRP glycopeptides and 28 IgG glycopeptides), and high selectivity (horseradish peroxidase/bovine serum albumin = 1:10 000).
29792669	7	3	gly	glycopeptides	973:985	arg2	glycopeptides					973:985	28 IgG glycopeptides	966:985	28 IgG glycopeptides	966:985	The gluCDMT-based monolith exhibited high binding capacity (∼50 mg g-1), good ability to capture glycopeptides (23 HRP glycopeptides and 28 IgG glycopeptides), and high selectivity (horseradish peroxidase/bovine serum albumin = 1:10 000).
29792669	0	4	theme	Glycopeptides	105:117	arg1	Enrichment					82:91	Specific Enrichment	73:91	Specific Enrichment of N-Linked Glycopeptides	73:117	Self-Assembling Glutamate-Functionalized Cyclodextrin Molecular Tube for Specific Enrichment of N-Linked Glycopeptides.
29792669	7	5	theme	IgG	969:971	arg1	glycopeptides					973:985	28 IgG glycopeptides	966:985	28 IgG glycopeptides	966:985	The gluCDMT-based monolith exhibited high binding capacity (∼50 mg g-1), good ability to capture glycopeptides (23 HRP glycopeptides and 28 IgG glycopeptides), and high selectivity (horseradish peroxidase/bovine serum albumin = 1:10 000).
29792669	1	6	theme	cyclodextrin	171:182	arg1	family					184:189	cyclodextrin family	171:189	cyclodextrin family	171:189	Cyclodextrin molecular tube (CDMT), a new comer of cyclodextrin family, possesses large and hydrophilic outer area and stable structure.
29792669	8	7	theme	cell	1180:1183	arg1	lysate					1185:1190	acute myelogenous leukemia cell lysate	1153:1190	acute myelogenous leukemia cell lysate	1153:1190	Moreover, the developed platform was successfully applied to analyze glycopetides in acute myelogenous leukemia cell lysate and human serum samples.
29792669	6	8	theme	surface	802:808	arg1	sites					822:826	the surface hydrophilic sites	798:826	the surface hydrophilic sites	798:826	A solid monolithic support was employed to immobilize gluCDMT by a host-guest self-assembly synthetic strategy, which did not occupy the surface hydrophilic sites.
29792669	5	9	theme	high	613:616	arg1	stability					618:626	high stability	613:626	high stability	613:626	The prepared gluCDMT exhibited large hydrophilic surface, high stability, and good acidic/alkalic resistance.
29792669	8	10	theme	leukemia	1171:1178	arg1	lysate					1185:1190	acute myelogenous leukemia cell lysate	1153:1190	acute myelogenous leukemia cell lysate	1153:1190	Moreover, the developed platform was successfully applied to analyze glycopetides in acute myelogenous leukemia cell lysate and human serum samples.
29792669	7	11	theme	∼50	889:891	arg1	mg					893:894	mg	893:894	mg	893:894	The gluCDMT-based monolith exhibited high binding capacity (∼50 mg g-1), good ability to capture glycopeptides (23 HRP glycopeptides and 28 IgG glycopeptides), and high selectivity (horseradish peroxidase/bovine serum albumin = 1:10 000).
29792669	7	12	dep	=	1055:1055	arg1	1:10 000					1057:1064	1:10 000	1057:1064	1:10 000	1057:1064	The gluCDMT-based monolith exhibited high binding capacity (∼50 mg g-1), good ability to capture glycopeptides (23 HRP glycopeptides and 28 IgG glycopeptides), and high selectivity (horseradish peroxidase/bovine serum albumin = 1:10 000).
29792669	7	13	dep	selectivity	998:1008	arg1	albumin					1047:1053	horseradish peroxidase/bovine serum albumin	1011:1053	horseradish peroxidase/bovine serum albumin = 1:10 000	1011:1064	The gluCDMT-based monolith exhibited high binding capacity (∼50 mg g-1), good ability to capture glycopeptides (23 HRP glycopeptides and 28 IgG glycopeptides), and high selectivity (horseradish peroxidase/bovine serum albumin = 1:10 000).
29792669	7	14	dep	glycopeptides	926:938	arg1	glycopeptides					948:960	23 HRP glycopeptides	941:960	23 HRP glycopeptides	941:960	The gluCDMT-based monolith exhibited high binding capacity (∼50 mg g-1), good ability to capture glycopeptides (23 HRP glycopeptides and 28 IgG glycopeptides), and high selectivity (horseradish peroxidase/bovine serum albumin = 1:10 000).
29792669	7	14	dep	glycopeptides	926:938	arg1	glycopeptides					973:985	28 IgG glycopeptides	966:985	28 IgG glycopeptides	966:985	The gluCDMT-based monolith exhibited high binding capacity (∼50 mg g-1), good ability to capture glycopeptides (23 HRP glycopeptides and 28 IgG glycopeptides), and high selectivity (horseradish peroxidase/bovine serum albumin = 1:10 000).
29792669	7	15	theme	mg	893:894	arg1	g-1					896:898	∼50 mg g-1	889:898	∼50 mg g-1	889:898	The gluCDMT-based monolith exhibited high binding capacity (∼50 mg g-1), good ability to capture glycopeptides (23 HRP glycopeptides and 28 IgG glycopeptides), and high selectivity (horseradish peroxidase/bovine serum albumin = 1:10 000).
29792669	7	15	theme	mg	893:894	arg1	capacity					879:886	high binding capacity	866:886	high binding capacity (∼50 mg g-1)	866:899	The gluCDMT-based monolith exhibited high binding capacity (∼50 mg g-1), good ability to capture glycopeptides (23 HRP glycopeptides and 28 IgG glycopeptides), and high selectivity (horseradish peroxidase/bovine serum albumin = 1:10 000).
29792669	1	16	theme	family	184:189	arg1	comer					162:166	a new comer	156:166	a new comer of cyclodextrin family	156:189	Cyclodextrin molecular tube (CDMT), a new comer of cyclodextrin family, possesses large and hydrophilic outer area and stable structure.
29792669	1	16	theme	family	184:189	arg1	tube					143:146	Cyclodextrin molecular tube	120:146	Cyclodextrin molecular tube (CDMT)	120:153	Cyclodextrin molecular tube (CDMT), a new comer of cyclodextrin family, possesses large and hydrophilic outer area and stable structure.
29792669	5	17	theme	good	633:636	arg1	resistance					653:662	good acidic/alkalic resistance	633:662	good acidic/alkalic resistance	633:662	The prepared gluCDMT exhibited large hydrophilic surface, high stability, and good acidic/alkalic resistance.
29792669	6	18	theme	hydrophilic	810:820	arg1	sites					822:826	the surface hydrophilic sites	798:826	the surface hydrophilic sites	798:826	A solid monolithic support was employed to immobilize gluCDMT by a host-guest self-assembly synthetic strategy, which did not occupy the surface hydrophilic sites.
29792669	0	19	theme	Self-Assembling	0:14	arg1	Tube					64:67	Self-Assembling Glutamate-Functionalized Cyclodextrin Molecular Tube	0:67	Self-Assembling Glutamate-Functionalized Cyclodextrin Molecular Tube for Specific Enrichment of N-Linked Glycopeptides.	0:118	Self-Assembling Glutamate-Functionalized Cyclodextrin Molecular Tube for Specific Enrichment of N-Linked Glycopeptides.
29792669	3	20	theme	glycopeptides	457:469	arg1	capture					446:452	the specific capture	433:452	the specific capture of glycopeptides	433:469	Herein, we developed a CDMT-based enrichment platform focusing on the specific capture of glycopeptides.
29792669	0	21	theme	Glutamate-Functionalized	16:39	arg1	Tube					64:67	Self-Assembling Glutamate-Functionalized Cyclodextrin Molecular Tube	0:67	Self-Assembling Glutamate-Functionalized Cyclodextrin Molecular Tube for Specific Enrichment of N-Linked Glycopeptides.	0:118	Self-Assembling Glutamate-Functionalized Cyclodextrin Molecular Tube for Specific Enrichment of N-Linked Glycopeptides.
29792669	4	22	theme	CDMT	505:508	arg1	hydrophilicity					487:500	the hydrophilicity	483:500	the hydrophilicity of CDMT	483:508	To enhance the hydrophilicity of CDMT, it was functionalized with glutamate (glu).
29792669	5	23	theme	acidic/alkalic	638:651	arg1	resistance					653:662	good acidic/alkalic resistance	633:662	good acidic/alkalic resistance	633:662	The prepared gluCDMT exhibited large hydrophilic surface, high stability, and good acidic/alkalic resistance.
29792669	5	24	theme	large	586:590	arg1	surface					604:610	large hydrophilic surface	586:610	large hydrophilic surface	586:610	The prepared gluCDMT exhibited large hydrophilic surface, high stability, and good acidic/alkalic resistance.
29792669	1	25	theme	Cyclodextrin	120:131	arg1	comer					162:166	a new comer	156:166	a new comer of cyclodextrin family	156:189	Cyclodextrin molecular tube (CDMT), a new comer of cyclodextrin family, possesses large and hydrophilic outer area and stable structure.
29792669	1	25	theme	Cyclodextrin	120:131	arg1	CDMT					149:152	CDMT	149:152	CDMT	149:152	Cyclodextrin molecular tube (CDMT), a new comer of cyclodextrin family, possesses large and hydrophilic outer area and stable structure.
29792669	1	25	theme	Cyclodextrin	120:131	arg1	tube					143:146	Cyclodextrin molecular tube	120:146	Cyclodextrin molecular tube (CDMT)	120:153	Cyclodextrin molecular tube (CDMT), a new comer of cyclodextrin family, possesses large and hydrophilic outer area and stable structure.
29792669	3	26	theme	specific	437:444	arg1	capture					446:452	the specific capture	433:452	the specific capture of glycopeptides	433:469	Herein, we developed a CDMT-based enrichment platform focusing on the specific capture of glycopeptides.
29792669	3	27	theme	enrichment	401:410	arg1	platform					412:419	a CDMT-based enrichment platform	388:419	a CDMT-based enrichment platform focusing on the specific capture of glycopeptides	388:469	Herein, we developed a CDMT-based enrichment platform focusing on the specific capture of glycopeptides.
29792669	5	28	theme	hydrophilic	592:602	arg1	surface					604:610	large hydrophilic surface	586:610	large hydrophilic surface	586:610	The prepared gluCDMT exhibited large hydrophilic surface, high stability, and good acidic/alkalic resistance.
29792669	1	29	theme	molecular	133:141	arg1	comer					162:166	a new comer	156:166	a new comer of cyclodextrin family	156:189	Cyclodextrin molecular tube (CDMT), a new comer of cyclodextrin family, possesses large and hydrophilic outer area and stable structure.
29792669	1	29	theme	molecular	133:141	arg1	CDMT					149:152	CDMT	149:152	CDMT	149:152	Cyclodextrin molecular tube (CDMT), a new comer of cyclodextrin family, possesses large and hydrophilic outer area and stable structure.
29792669	1	29	theme	molecular	133:141	arg1	tube					143:146	Cyclodextrin molecular tube	120:146	Cyclodextrin molecular tube (CDMT)	120:153	Cyclodextrin molecular tube (CDMT), a new comer of cyclodextrin family, possesses large and hydrophilic outer area and stable structure.
29792669	7	30	theme	high	866:869	arg1	g-1					896:898	∼50 mg g-1	889:898	∼50 mg g-1	889:898	The gluCDMT-based monolith exhibited high binding capacity (∼50 mg g-1), good ability to capture glycopeptides (23 HRP glycopeptides and 28 IgG glycopeptides), and high selectivity (horseradish peroxidase/bovine serum albumin = 1:10 000).
29792669	7	30	theme	high	866:869	arg1	capacity					879:886	high binding capacity	866:886	high binding capacity (∼50 mg g-1)	866:899	The gluCDMT-based monolith exhibited high binding capacity (∼50 mg g-1), good ability to capture glycopeptides (23 HRP glycopeptides and 28 IgG glycopeptides), and high selectivity (horseradish peroxidase/bovine serum albumin = 1:10 000).
29792669	1	31	theme	large	202:206	arg1	area					230:233	outer area	224:233	outer area	224:233	Cyclodextrin molecular tube (CDMT), a new comer of cyclodextrin family, possesses large and hydrophilic outer area and stable structure.
29792669	0	32	theme	Molecular	54:62	arg1	Tube					64:67	Self-Assembling Glutamate-Functionalized Cyclodextrin Molecular Tube	0:67	Self-Assembling Glutamate-Functionalized Cyclodextrin Molecular Tube for Specific Enrichment of N-Linked Glycopeptides.	0:118	Self-Assembling Glutamate-Functionalized Cyclodextrin Molecular Tube for Specific Enrichment of N-Linked Glycopeptides.
29792669	7	33	theme	gluCDMT-based	833:845	arg1	monolith					847:854	The gluCDMT-based monolith	829:854	The gluCDMT-based monolith	829:854	The gluCDMT-based monolith exhibited high binding capacity (∼50 mg g-1), good ability to capture glycopeptides (23 HRP glycopeptides and 28 IgG glycopeptides), and high selectivity (horseradish peroxidase/bovine serum albumin = 1:10 000).
29792669	8	34	theme	developed	1082:1090	arg1	platform					1092:1099	the developed platform	1078:1099	the developed platform	1078:1099	Moreover, the developed platform was successfully applied to analyze glycopetides in acute myelogenous leukemia cell lysate and human serum samples.
29792669	7	35	theme	binding	871:877	arg1	g-1					896:898	∼50 mg g-1	889:898	∼50 mg g-1	889:898	The gluCDMT-based monolith exhibited high binding capacity (∼50 mg g-1), good ability to capture glycopeptides (23 HRP glycopeptides and 28 IgG glycopeptides), and high selectivity (horseradish peroxidase/bovine serum albumin = 1:10 000).
29792669	7	35	theme	binding	871:877	arg1	capacity					879:886	high binding capacity	866:886	high binding capacity (∼50 mg g-1)	866:899	The gluCDMT-based monolith exhibited high binding capacity (∼50 mg g-1), good ability to capture glycopeptides (23 HRP glycopeptides and 28 IgG glycopeptides), and high selectivity (horseradish peroxidase/bovine serum albumin = 1:10 000).
29792669	0	36	theme	Cyclodextrin	41:52	arg1	Tube					64:67	Self-Assembling Glutamate-Functionalized Cyclodextrin Molecular Tube	0:67	Self-Assembling Glutamate-Functionalized Cyclodextrin Molecular Tube for Specific Enrichment of N-Linked Glycopeptides.	0:118	Self-Assembling Glutamate-Functionalized Cyclodextrin Molecular Tube for Specific Enrichment of N-Linked Glycopeptides.
29792669	1	37	theme	hydrophilic	212:222	arg1	area					230:233	outer area	224:233	outer area	224:233	Cyclodextrin molecular tube (CDMT), a new comer of cyclodextrin family, possesses large and hydrophilic outer area and stable structure.
29792669	1	38	contain	possesses	192:200	arg2	area					230:233	outer area	224:233	outer area	224:233	Cyclodextrin molecular tube (CDMT), a new comer of cyclodextrin family, possesses large and hydrophilic outer area and stable structure.
29792669	1	38	contain	possesses	192:200	arg1	comer					162:166	a new comer	156:166	a new comer of cyclodextrin family	156:189	Cyclodextrin molecular tube (CDMT), a new comer of cyclodextrin family, possesses large and hydrophilic outer area and stable structure.
29792669	1	38	contain	possesses	192:200	arg1	CDMT					149:152	CDMT	149:152	CDMT	149:152	Cyclodextrin molecular tube (CDMT), a new comer of cyclodextrin family, possesses large and hydrophilic outer area and stable structure.
29792669	1	38	contain	possesses	192:200	arg1	tube					143:146	Cyclodextrin molecular tube	120:146	Cyclodextrin molecular tube (CDMT)	120:153	Cyclodextrin molecular tube (CDMT), a new comer of cyclodextrin family, possesses large and hydrophilic outer area and stable structure.
29792669	1	38	contain	possesses	192:200	arg2	structure					246:254	stable structure	239:254	stable structure	239:254	Cyclodextrin molecular tube (CDMT), a new comer of cyclodextrin family, possesses large and hydrophilic outer area and stable structure.
29792669	1	39	theme	outer	224:228	arg1	area					230:233	outer area	224:233	outer area	224:233	Cyclodextrin molecular tube (CDMT), a new comer of cyclodextrin family, possesses large and hydrophilic outer area and stable structure.
29792669	8	40	theme	acute	1153:1157	arg1	leukemia					1171:1178	acute myelogenous leukemia	1153:1178	acute myelogenous leukemia cell lysate	1153:1190	Moreover, the developed platform was successfully applied to analyze glycopetides in acute myelogenous leukemia cell lysate and human serum samples.
29792669	7	41	theme	horseradish	1011:1021	arg1	albumin					1047:1053	horseradish peroxidase/bovine serum albumin	1011:1053	horseradish peroxidase/bovine serum albumin = 1:10 000	1011:1064	The gluCDMT-based monolith exhibited high binding capacity (∼50 mg g-1), good ability to capture glycopeptides (23 HRP glycopeptides and 28 IgG glycopeptides), and high selectivity (horseradish peroxidase/bovine serum albumin = 1:10 000).
29792669	8	42	theme	serum	1202:1206	arg1	samples					1208:1214	human serum samples	1196:1214	human serum samples	1196:1214	Moreover, the developed platform was successfully applied to analyze glycopetides in acute myelogenous leukemia cell lysate and human serum samples.
29792669	7	43	dep	ability	907:913	arg1	capture					918:924	capture	918:924	to capture glycopeptides (23 HRP glycopeptides and 28 IgG glycopeptides)	915:986	The gluCDMT-based monolith exhibited high binding capacity (∼50 mg g-1), good ability to capture glycopeptides (23 HRP glycopeptides and 28 IgG glycopeptides), and high selectivity (horseradish peroxidase/bovine serum albumin = 1:10 000).
29792669	7	44	theme	=	1055:1055	arg1	albumin					1047:1053	horseradish peroxidase/bovine serum albumin	1011:1053	horseradish peroxidase/bovine serum albumin = 1:10 000	1011:1064	The gluCDMT-based monolith exhibited high binding capacity (∼50 mg g-1), good ability to capture glycopeptides (23 HRP glycopeptides and 28 IgG glycopeptides), and high selectivity (horseradish peroxidase/bovine serum albumin = 1:10 000).
29792669	8	45	from	glycopetides	1137:1148	arg1	samples					1208:1214	human serum samples	1196:1214	human serum samples	1196:1214	Moreover, the developed platform was successfully applied to analyze glycopetides in acute myelogenous leukemia cell lysate and human serum samples.
29792669	8	45	from	glycopetides	1137:1148	arg1	lysate					1185:1190	acute myelogenous leukemia cell lysate	1153:1190	acute myelogenous leukemia cell lysate	1153:1190	Moreover, the developed platform was successfully applied to analyze glycopetides in acute myelogenous leukemia cell lysate and human serum samples.
29792669	7	46	theme	peroxidase/bovine	1023:1039	arg1	albumin					1047:1053	horseradish peroxidase/bovine serum albumin	1011:1053	horseradish peroxidase/bovine serum albumin = 1:10 000	1011:1064	The gluCDMT-based monolith exhibited high binding capacity (∼50 mg g-1), good ability to capture glycopeptides (23 HRP glycopeptides and 28 IgG glycopeptides), and high selectivity (horseradish peroxidase/bovine serum albumin = 1:10 000).
29792669	2	47	theme	enrichment	355:364	arg1	field					331:335	the field	327:335	the field of separation and enrichment	327:364	Its development and applications remain highly desired, especially in the field of separation and enrichment.
29792669	0	48	theme	Specific	73:80	arg1	Enrichment					82:91	Specific Enrichment	73:91	Specific Enrichment of N-Linked Glycopeptides	73:117	Self-Assembling Glutamate-Functionalized Cyclodextrin Molecular Tube for Specific Enrichment of N-Linked Glycopeptides.
29792669	7	49	gly	glycopeptides	926:938	arg2	glycopeptides					926:938	glycopeptides	926:938	glycopeptides (23 HRP glycopeptides and 28 IgG glycopeptides)	926:986	The gluCDMT-based monolith exhibited high binding capacity (∼50 mg g-1), good ability to capture glycopeptides (23 HRP glycopeptides and 28 IgG glycopeptides), and high selectivity (horseradish peroxidase/bovine serum albumin = 1:10 000).
29792669	5	50	theme	prepared	559:566	arg1	gluCDMT					568:574	The prepared gluCDMT	555:574	The prepared gluCDMT	555:574	The prepared gluCDMT exhibited large hydrophilic surface, high stability, and good acidic/alkalic resistance.
29792669	6	51	theme	monolithic	673:682	arg1	support					684:690	A solid monolithic support	665:690	A solid monolithic support	665:690	A solid monolithic support was employed to immobilize gluCDMT by a host-guest self-assembly synthetic strategy, which did not occupy the surface hydrophilic sites.
29792669	7	52	theme	high	993:996	arg1	selectivity					998:1008	high selectivity	993:1008	high selectivity (horseradish peroxidase/bovine serum albumin = 1:10 000)	993:1065	The gluCDMT-based monolith exhibited high binding capacity (∼50 mg g-1), good ability to capture glycopeptides (23 HRP glycopeptides and 28 IgG glycopeptides), and high selectivity (horseradish peroxidase/bovine serum albumin = 1:10 000).
29792669	7	53	theme	serum	1041:1045	arg1	albumin					1047:1053	horseradish peroxidase/bovine serum albumin	1011:1053	horseradish peroxidase/bovine serum albumin = 1:10 000	1011:1064	The gluCDMT-based monolith exhibited high binding capacity (∼50 mg g-1), good ability to capture glycopeptides (23 HRP glycopeptides and 28 IgG glycopeptides), and high selectivity (horseradish peroxidase/bovine serum albumin = 1:10 000).
29792669	7	54	theme	HRP	944:946	arg1	glycopeptides					948:960	23 HRP glycopeptides	941:960	23 HRP glycopeptides	941:960	The gluCDMT-based monolith exhibited high binding capacity (∼50 mg g-1), good ability to capture glycopeptides (23 HRP glycopeptides and 28 IgG glycopeptides), and high selectivity (horseradish peroxidase/bovine serum albumin = 1:10 000).
29792669	3	55	gly	glycopeptides	457:469	arg2	glycopeptides					457:469	glycopeptides	457:469	glycopeptides	457:469	Herein, we developed a CDMT-based enrichment platform focusing on the specific capture of glycopeptides.
29792669	2	56	theme	separation	340:349	arg1	field					331:335	the field	327:335	the field of separation and enrichment	327:364	Its development and applications remain highly desired, especially in the field of separation and enrichment.
29792669	6	57	theme	solid	667:671	arg1	support					684:690	A solid monolithic support	665:690	A solid monolithic support	665:690	A solid monolithic support was employed to immobilize gluCDMT by a host-guest self-assembly synthetic strategy, which did not occupy the surface hydrophilic sites.
29792669	7	58	gly	glycopeptides	948:960	arg2	glycopeptides					948:960	23 HRP glycopeptides	941:960	23 HRP glycopeptides	941:960	The gluCDMT-based monolith exhibited high binding capacity (∼50 mg g-1), good ability to capture glycopeptides (23 HRP glycopeptides and 28 IgG glycopeptides), and high selectivity (horseradish peroxidase/bovine serum albumin = 1:10 000).
29792669	8	59	theme	human	1196:1200	arg1	samples					1208:1214	human serum samples	1196:1214	human serum samples	1196:1214	Moreover, the developed platform was successfully applied to analyze glycopetides in acute myelogenous leukemia cell lysate and human serum samples.
29792669	1	60	theme	stable	239:244	arg1	structure					246:254	stable structure	239:254	stable structure	239:254	Cyclodextrin molecular tube (CDMT), a new comer of cyclodextrin family, possesses large and hydrophilic outer area and stable structure.
29792669	0	61	theme	N-Linked	96:103	arg1	Glycopeptides					105:117	N-Linked Glycopeptides	96:117	N-Linked Glycopeptides	96:117	Self-Assembling Glutamate-Functionalized Cyclodextrin Molecular Tube for Specific Enrichment of N-Linked Glycopeptides.
29792669	6	62	theme	synthetic	757:765	arg1	strategy					767:774	a host-guest self-assembly synthetic strategy	730:774	a host-guest self-assembly synthetic strategy	730:774	A solid monolithic support was employed to immobilize gluCDMT by a host-guest self-assembly synthetic strategy, which did not occupy the surface hydrophilic sites.
29792669	1	63	theme	new	158:160	arg1	comer					162:166	a new comer	156:166	a new comer of cyclodextrin family	156:189	Cyclodextrin molecular tube (CDMT), a new comer of cyclodextrin family, possesses large and hydrophilic outer area and stable structure.
29792669	1	63	theme	new	158:160	arg1	tube					143:146	Cyclodextrin molecular tube	120:146	Cyclodextrin molecular tube (CDMT)	120:153	Cyclodextrin molecular tube (CDMT), a new comer of cyclodextrin family, possesses large and hydrophilic outer area and stable structure.
29792669	6	64	theme	self-assembly	743:755	arg1	strategy					767:774	a host-guest self-assembly synthetic strategy	730:774	a host-guest self-assembly synthetic strategy	730:774	A solid monolithic support was employed to immobilize gluCDMT by a host-guest self-assembly synthetic strategy, which did not occupy the surface hydrophilic sites.
29792669	3	65	theme	CDMT-based	390:399	arg1	platform					412:419	a CDMT-based enrichment platform	388:419	a CDMT-based enrichment platform focusing on the specific capture of glycopeptides	388:469	Herein, we developed a CDMT-based enrichment platform focusing on the specific capture of glycopeptides.
31909333	0	0	theme	Dye	85:87	arg1	Pollutants					89:98	Dye Pollutants	85:98	Dye Pollutants	85:98	Crystalline Covalent Organic Frameworks from Triazine Nodes as Porous Adsorbents for Dye Pollutants.
31909333	4	1	theme	O-linked	719:726	arg1	-1,3,5-triazine					764:778	O-linked flexible 2,4,6-tris(p-formylphenoxy)-1,3,5-triazine	719:778	O-linked flexible 2,4,6-tris(p-formylphenoxy)-1,3,5-triazine	719:778	In this study, the COF prepared from O-linked flexible 2,4,6-tris(p-formylphenoxy)-1,3,5-triazine serves as an excellent absorbent for removing methylene blue from water.
31909333	5	2	from	stable	896:901	arg1	water					906:910	water	906:910	water	906:910	Our results demonstrate that COF is highly stable in water and functions as a robust adsorbent.
31909333	3	3	theme	triazine	516:523	arg1	nodes					525:529	flat, rigid sp2 carbon-linked triazine nodes	486:529	flat, rigid sp2 carbon-linked triazine nodes	486:529	Compared to COFs prepared from flat, rigid sp2 carbon-linked triazine nodes, the O-linked flexible tripodal triazine-based COF demonstrates several unpredictable properties such as an increase in crystallinity and cavity size.
31909333	4	4	attach	removing	817:824	arg2	COF					701:703	the COF	697:703	the COF prepared from O-linked flexible 2,4,6-tris(p-formylphenoxy)-1,3,5-triazine	697:778	In this study, the COF prepared from O-linked flexible 2,4,6-tris(p-formylphenoxy)-1,3,5-triazine serves as an excellent absorbent for removing methylene blue from water.
31909333	4	4	attach	removing	817:824	arg2	absorbent					803:811	an excellent absorbent	790:811	an excellent absorbent for removing methylene blue from water	790:850	In this study, the COF prepared from O-linked flexible 2,4,6-tris(p-formylphenoxy)-1,3,5-triazine serves as an excellent absorbent for removing methylene blue from water.
31909333	4	4	attach	removing	817:824	arg1	water					846:850	water	846:850	water	846:850	In this study, the COF prepared from O-linked flexible 2,4,6-tris(p-formylphenoxy)-1,3,5-triazine serves as an excellent absorbent for removing methylene blue from water.
31909333	7	5	theme	X-ray	1287:1291	arg1	diffraction					1293:1303	X-ray diffraction	1287:1303	X-ray diffraction	1287:1303	Moreover, the COF was characterized using elemental analysis, Fourier transform infrared spectroscopy, thermogravimetric analysis, scanning electron microscopy, solid-state ultraviolet-visible spectroscopy, and X-ray diffraction.
31909333	7	6	dep	Fourier	1138:1144	arg1	transform					1146:1154	transform	1146:1154	transform infrared spectroscopy	1146:1176	Moreover, the COF was characterized using elemental analysis, Fourier transform infrared spectroscopy, thermogravimetric analysis, scanning electron microscopy, solid-state ultraviolet-visible spectroscopy, and X-ray diffraction.
31909333	9	7	theme	semiconducting	1532:1545	arg1	nature					1547:1552	its semiconducting nature	1528:1552	its semiconducting nature	1528:1552	Photophysical studies revealed that the COF exhibits a low bandgap energy value of 3.07 eV, indicating its semiconducting nature.
31909333	6	8	theme	adsorption	1019:1028	arg1	kinetics					1030:1037	its adsorption kinetics	1015:1037	its adsorption kinetics	1015:1037	Its adsorption isotherm is consistent with the Langmuir model and its adsorption kinetics follows a pseudo-second order model.
31909333	3	9	link	O-linked	536:543	arg1	COF					578:580	the O-linked flexible tripodal triazine-based COF	532:580	the O-linked flexible tripodal triazine-based COF	532:580	Compared to COFs prepared from flat, rigid sp2 carbon-linked triazine nodes, the O-linked flexible tripodal triazine-based COF demonstrates several unpredictable properties such as an increase in crystallinity and cavity size.
31909333	7	10	dep	transform	1146:1154	arg1	infrared					1156:1163	infrared	1156:1163	transform infrared spectroscopy	1146:1176	Moreover, the COF was characterized using elemental analysis, Fourier transform infrared spectroscopy, thermogravimetric analysis, scanning electron microscopy, solid-state ultraviolet-visible spectroscopy, and X-ray diffraction.
31909333	3	11	theme	tripodal	554:561	arg1	COF					578:580	the O-linked flexible tripodal triazine-based COF	532:580	the O-linked flexible tripodal triazine-based COF	532:580	Compared to COFs prepared from flat, rigid sp2 carbon-linked triazine nodes, the O-linked flexible tripodal triazine-based COF demonstrates several unpredictable properties such as an increase in crystallinity and cavity size.
31909333	9	12	theme	low	1480:1482	arg1	value					1499:1503	a low bandgap energy value	1478:1503	a low bandgap energy value of 3.07 eV	1478:1514	Photophysical studies revealed that the COF exhibits a low bandgap energy value of 3.07 eV, indicating its semiconducting nature.
31909333	4	13	link	O-linked	719:726	arg1	-1,3,5-triazine					764:778	O-linked flexible 2,4,6-tris(p-formylphenoxy)-1,3,5-triazine	719:778	O-linked flexible 2,4,6-tris(p-formylphenoxy)-1,3,5-triazine	719:778	In this study, the COF prepared from O-linked flexible 2,4,6-tris(p-formylphenoxy)-1,3,5-triazine serves as an excellent absorbent for removing methylene blue from water.
31909333	9	14	theme	eV	1513:1514	arg1	value					1499:1503	a low bandgap energy value	1478:1503	a low bandgap energy value of 3.07 eV	1478:1514	Photophysical studies revealed that the COF exhibits a low bandgap energy value of 3.07 eV, indicating its semiconducting nature.
31909333	3	15	theme	triazine-based	563:576	arg1	COF					578:580	the O-linked flexible tripodal triazine-based COF	532:580	the O-linked flexible tripodal triazine-based COF	532:580	Compared to COFs prepared from flat, rigid sp2 carbon-linked triazine nodes, the O-linked flexible tripodal triazine-based COF demonstrates several unpredictable properties such as an increase in crystallinity and cavity size.
31909333	2	16	theme	electron	270:277	arg1	affinity					279:286	better electron affinity	263:286	better electron affinity than benzene-based aromatic rings	263:320	Triazines exhibit better electron affinity than benzene-based aromatic rings; therefore, structures based on 1,3,5-substituted triazine-centered nodes are more stable than those from 1,3,5-benzene-linked COFs.
31909333	3	17	from	increase	639:646	arg1	crystallinity					651:663	crystallinity	651:663	crystallinity	651:663	Compared to COFs prepared from flat, rigid sp2 carbon-linked triazine nodes, the O-linked flexible tripodal triazine-based COF demonstrates several unpredictable properties such as an increase in crystallinity and cavity size.
31909333	3	17	from	increase	639:646	arg1	size					676:679	cavity size	669:679	cavity size	669:679	Compared to COFs prepared from flat, rigid sp2 carbon-linked triazine nodes, the O-linked flexible tripodal triazine-based COF demonstrates several unpredictable properties such as an increase in crystallinity and cavity size.
31909333	9	18	theme	bandgap	1484:1490	arg1	value					1499:1503	a low bandgap energy value	1478:1503	a low bandgap energy value of 3.07 eV	1478:1514	Photophysical studies revealed that the COF exhibits a low bandgap energy value of 3.07 eV, indicating its semiconducting nature.
31909333	7	19	theme	solid-state	1237:1247	arg1	spectroscopy					1269:1280	solid-state ultraviolet-visible spectroscopy	1237:1280	solid-state ultraviolet-visible spectroscopy	1237:1280	Moreover, the COF was characterized using elemental analysis, Fourier transform infrared spectroscopy, thermogravimetric analysis, scanning electron microscopy, solid-state ultraviolet-visible spectroscopy, and X-ray diffraction.
31909333	0	20	from	Nodes	54:58	arg1	Frameworks					29:38	Crystalline Covalent Organic Frameworks	0:38	Crystalline Covalent Organic Frameworks from Triazine Nodes as Porous Adsorbents for Dye Pollutants.	0:99	Crystalline Covalent Organic Frameworks from Triazine Nodes as Porous Adsorbents for Dye Pollutants.
31909333	7	21	theme	electron	1216:1223	arg1	microscopy					1225:1234	scanning electron microscopy	1207:1234	scanning electron microscopy	1207:1234	Moreover, the COF was characterized using elemental analysis, Fourier transform infrared spectroscopy, thermogravimetric analysis, scanning electron microscopy, solid-state ultraviolet-visible spectroscopy, and X-ray diffraction.
31909333	8	22	theme	surface	1355:1361	arg1	porosity					1329:1336	permanent porosity	1319:1336	permanent porosity	1319:1336	It exhibited permanent porosity, a high specific surface area (279.5 m2·g-1), and was chemically and thermally stable.
31909333	8	22	theme	surface	1355:1361	arg1	area					1363:1366	a high specific surface area	1339:1366	a high specific surface area (279.5 m2·g-1)	1339:1381	It exhibited permanent porosity, a high specific surface area (279.5 m2·g-1), and was chemically and thermally stable.
31909333	8	22	theme	surface	1355:1361	arg1	m2·g-1					1375:1380	279.5 m2·g-1	1369:1380	279.5 m2·g-1	1369:1380	It exhibited permanent porosity, a high specific surface area (279.5 m2·g-1), and was chemically and thermally stable.
31909333	2	23	theme	better	263:268	arg1	affinity					279:286	better electron affinity	263:286	better electron affinity than benzene-based aromatic rings	263:320	Triazines exhibit better electron affinity than benzene-based aromatic rings; therefore, structures based on 1,3,5-substituted triazine-centered nodes are more stable than those from 1,3,5-benzene-linked COFs.
31909333	9	24	theme	energy	1492:1497	arg1	value					1499:1503	a low bandgap energy value	1478:1503	a low bandgap energy value of 3.07 eV	1478:1514	Photophysical studies revealed that the COF exhibits a low bandgap energy value of 3.07 eV, indicating its semiconducting nature.
31909333	7	25	theme	ultraviolet-visible	1249:1267	arg1	spectroscopy					1269:1280	solid-state ultraviolet-visible spectroscopy	1237:1280	solid-state ultraviolet-visible spectroscopy	1237:1280	Moreover, the COF was characterized using elemental analysis, Fourier transform infrared spectroscopy, thermogravimetric analysis, scanning electron microscopy, solid-state ultraviolet-visible spectroscopy, and X-ray diffraction.
31909333	1	26	with	frameworks	137:146	arg1	spacers					170:176	spacers	170:176	spacers	170:176	The development of covalent organic frameworks (COFs) with nodes and spacers, designed to maximize their functional properties, is a challenge.
31909333	1	26	with	frameworks	137:146	arg1	nodes					160:164	nodes	160:164	nodes	160:164	The development of covalent organic frameworks (COFs) with nodes and spacers, designed to maximize their functional properties, is a challenge.
31909333	3	27	theme	flexible	545:552	arg1	COF					578:580	the O-linked flexible tripodal triazine-based COF	532:580	the O-linked flexible tripodal triazine-based COF	532:580	Compared to COFs prepared from flat, rigid sp2 carbon-linked triazine nodes, the O-linked flexible tripodal triazine-based COF demonstrates several unpredictable properties such as an increase in crystallinity and cavity size.
31909333	0	28	theme	Covalent	12:19	arg1	Frameworks					29:38	Crystalline Covalent Organic Frameworks	0:38	Crystalline Covalent Organic Frameworks from Triazine Nodes as Porous Adsorbents for Dye Pollutants.	0:99	Crystalline Covalent Organic Frameworks from Triazine Nodes as Porous Adsorbents for Dye Pollutants.
31909333	3	29	theme	several	595:601	arg1	properties					617:626	several unpredictable properties	595:626	several unpredictable properties such as an increase in crystallinity and cavity size	595:679	Compared to COFs prepared from flat, rigid sp2 carbon-linked triazine nodes, the O-linked flexible tripodal triazine-based COF demonstrates several unpredictable properties such as an increase in crystallinity and cavity size.
31909333	3	29	theme	several	595:601	arg1	increase					639:646	an increase	636:646	an increase in crystallinity and cavity size	636:679	Compared to COFs prepared from flat, rigid sp2 carbon-linked triazine nodes, the O-linked flexible tripodal triazine-based COF demonstrates several unpredictable properties such as an increase in crystallinity and cavity size.
31909333	0	30	theme	Crystalline	0:10	arg1	Frameworks					29:38	Crystalline Covalent Organic Frameworks	0:38	Crystalline Covalent Organic Frameworks from Triazine Nodes as Porous Adsorbents for Dye Pollutants.	0:99	Crystalline Covalent Organic Frameworks from Triazine Nodes as Porous Adsorbents for Dye Pollutants.
31909333	7	31	theme	elemental	1118:1126	arg1	analysis					1128:1135	elemental analysis	1118:1135	elemental analysis	1118:1135	Moreover, the COF was characterized using elemental analysis, Fourier transform infrared spectroscopy, thermogravimetric analysis, scanning electron microscopy, solid-state ultraviolet-visible spectroscopy, and X-ray diffraction.
31909333	2	32	theme	aromatic	307:314	arg1	rings					316:320	benzene-based aromatic rings	293:320	benzene-based aromatic rings	293:320	Triazines exhibit better electron affinity than benzene-based aromatic rings; therefore, structures based on 1,3,5-substituted triazine-centered nodes are more stable than those from 1,3,5-benzene-linked COFs.
31909333	8	33	theme	permanent	1319:1327	arg1	porosity					1329:1336	permanent porosity	1319:1336	permanent porosity	1319:1336	It exhibited permanent porosity, a high specific surface area (279.5 m2·g-1), and was chemically and thermally stable.
31909333	8	33	theme	permanent	1319:1327	arg1	area					1363:1366	a high specific surface area	1339:1366	a high specific surface area (279.5 m2·g-1)	1339:1381	It exhibited permanent porosity, a high specific surface area (279.5 m2·g-1), and was chemically and thermally stable.
31909333	7	34	theme	scanning	1207:1214	arg1	microscopy					1225:1234	scanning electron microscopy	1207:1234	scanning electron microscopy	1207:1234	Moreover, the COF was characterized using elemental analysis, Fourier transform infrared spectroscopy, thermogravimetric analysis, scanning electron microscopy, solid-state ultraviolet-visible spectroscopy, and X-ray diffraction.
31909333	6	35	theme	Langmuir	996:1003	arg1	model					1005:1009	the Langmuir model	992:1009	the Langmuir model	992:1009	Its adsorption isotherm is consistent with the Langmuir model and its adsorption kinetics follows a pseudo-second order model.
31909333	2	36	theme	benzene-based	293:305	arg1	rings					316:320	benzene-based aromatic rings	293:320	benzene-based aromatic rings	293:320	Triazines exhibit better electron affinity than benzene-based aromatic rings; therefore, structures based on 1,3,5-substituted triazine-centered nodes are more stable than those from 1,3,5-benzene-linked COFs.
31909333	2	37	theme	1,3,5-benzene-linked	428:447	arg1	COFs					449:452	1,3,5-benzene-linked COFs	428:452	1,3,5-benzene-linked COFs	428:452	Triazines exhibit better electron affinity than benzene-based aromatic rings; therefore, structures based on 1,3,5-substituted triazine-centered nodes are more stable than those from 1,3,5-benzene-linked COFs.
31909333	0	38	theme	Organic	21:27	arg1	Frameworks					29:38	Crystalline Covalent Organic Frameworks	0:38	Crystalline Covalent Organic Frameworks from Triazine Nodes as Porous Adsorbents for Dye Pollutants.	0:99	Crystalline Covalent Organic Frameworks from Triazine Nodes as Porous Adsorbents for Dye Pollutants.
31909333	3	39	theme	O-linked	536:543	arg1	COF					578:580	the O-linked flexible tripodal triazine-based COF	532:580	the O-linked flexible tripodal triazine-based COF	532:580	Compared to COFs prepared from flat, rigid sp2 carbon-linked triazine nodes, the O-linked flexible tripodal triazine-based COF demonstrates several unpredictable properties such as an increase in crystallinity and cavity size.
31909333	3	40	theme	flat	486:489	arg1	nodes					525:529	flat, rigid sp2 carbon-linked triazine nodes	486:529	flat, rigid sp2 carbon-linked triazine nodes	486:529	Compared to COFs prepared from flat, rigid sp2 carbon-linked triazine nodes, the O-linked flexible tripodal triazine-based COF demonstrates several unpredictable properties such as an increase in crystallinity and cavity size.
31909333	0	41	theme	Triazine	45:52	arg1	Nodes					54:58	Triazine Nodes	45:58	Triazine Nodes	45:58	Crystalline Covalent Organic Frameworks from Triazine Nodes as Porous Adsorbents for Dye Pollutants.
31909333	3	42	theme	carbon-linked	502:514	arg1	nodes					525:529	flat, rigid sp2 carbon-linked triazine nodes	486:529	flat, rigid sp2 carbon-linked triazine nodes	486:529	Compared to COFs prepared from flat, rigid sp2 carbon-linked triazine nodes, the O-linked flexible tripodal triazine-based COF demonstrates several unpredictable properties such as an increase in crystallinity and cavity size.
31909333	8	43	theme	specific	1346:1353	arg1	porosity					1329:1336	permanent porosity	1319:1336	permanent porosity	1319:1336	It exhibited permanent porosity, a high specific surface area (279.5 m2·g-1), and was chemically and thermally stable.
31909333	8	43	theme	specific	1346:1353	arg1	area					1363:1366	a high specific surface area	1339:1366	a high specific surface area (279.5 m2·g-1)	1339:1381	It exhibited permanent porosity, a high specific surface area (279.5 m2·g-1), and was chemically and thermally stable.
31909333	8	43	theme	specific	1346:1353	arg1	m2·g-1					1375:1380	279.5 m2·g-1	1369:1380	279.5 m2·g-1	1369:1380	It exhibited permanent porosity, a high specific surface area (279.5 m2·g-1), and was chemically and thermally stable.
31909333	3	44	theme	unpredictable	603:615	arg1	properties					617:626	several unpredictable properties	595:626	several unpredictable properties such as an increase in crystallinity and cavity size	595:679	Compared to COFs prepared from flat, rigid sp2 carbon-linked triazine nodes, the O-linked flexible tripodal triazine-based COF demonstrates several unpredictable properties such as an increase in crystallinity and cavity size.
31909333	3	44	theme	unpredictable	603:615	arg1	increase					639:646	an increase	636:646	an increase in crystallinity and cavity size	636:679	Compared to COFs prepared from flat, rigid sp2 carbon-linked triazine nodes, the O-linked flexible tripodal triazine-based COF demonstrates several unpredictable properties such as an increase in crystallinity and cavity size.
31909333	6	45	theme	order	1063:1067	arg1	model					1069:1073	a pseudo-second order model	1047:1073	a pseudo-second order model	1047:1073	Its adsorption isotherm is consistent with the Langmuir model and its adsorption kinetics follows a pseudo-second order model.
31909333	3	46	theme	cavity	669:674	arg1	size					676:679	cavity size	669:679	cavity size	669:679	Compared to COFs prepared from flat, rigid sp2 carbon-linked triazine nodes, the O-linked flexible tripodal triazine-based COF demonstrates several unpredictable properties such as an increase in crystallinity and cavity size.
31909333	8	47	theme	high	1341:1344	arg1	porosity					1329:1336	permanent porosity	1319:1336	permanent porosity	1319:1336	It exhibited permanent porosity, a high specific surface area (279.5 m2·g-1), and was chemically and thermally stable.
31909333	8	47	theme	high	1341:1344	arg1	area					1363:1366	a high specific surface area	1339:1366	a high specific surface area (279.5 m2·g-1)	1339:1381	It exhibited permanent porosity, a high specific surface area (279.5 m2·g-1), and was chemically and thermally stable.
31909333	8	47	theme	high	1341:1344	arg1	m2·g-1					1375:1380	279.5 m2·g-1	1369:1380	279.5 m2·g-1	1369:1380	It exhibited permanent porosity, a high specific surface area (279.5 m2·g-1), and was chemically and thermally stable.
31909333	3	48	theme	sp2	498:500	arg1	nodes					525:529	flat, rigid sp2 carbon-linked triazine nodes	486:529	flat, rigid sp2 carbon-linked triazine nodes	486:529	Compared to COFs prepared from flat, rigid sp2 carbon-linked triazine nodes, the O-linked flexible tripodal triazine-based COF demonstrates several unpredictable properties such as an increase in crystallinity and cavity size.
31909333	2	49	theme	1,3,5-substituted	354:370	arg1	nodes					390:394	1,3,5-substituted triazine-centered nodes	354:394	1,3,5-substituted triazine-centered nodes	354:394	Triazines exhibit better electron affinity than benzene-based aromatic rings; therefore, structures based on 1,3,5-substituted triazine-centered nodes are more stable than those from 1,3,5-benzene-linked COFs.
31909333	9	50	theme	Photophysical	1425:1437	arg1	studies					1439:1445	Photophysical studies	1425:1445	Photophysical studies	1425:1445	Photophysical studies revealed that the COF exhibits a low bandgap energy value of 3.07 eV, indicating its semiconducting nature.
31909333	1	51	theme	covalent	120:127	arg1	COFs					149:152	COFs	149:152	COFs	149:152	The development of covalent organic frameworks (COFs) with nodes and spacers, designed to maximize their functional properties, is a challenge.
31909333	1	51	theme	covalent	120:127	arg1	frameworks					137:146	covalent organic frameworks	120:146	covalent organic frameworks (COFs) with nodes and spacers	120:176	The development of covalent organic frameworks (COFs) with nodes and spacers, designed to maximize their functional properties, is a challenge.
31909333	6	52	theme	adsorption	953:962	arg1	isotherm					964:971	Its adsorption isotherm	949:971	Its adsorption isotherm	949:971	Its adsorption isotherm is consistent with the Langmuir model and its adsorption kinetics follows a pseudo-second order model.
31909333	4	53	theme	2,4,6-tris	737:746	arg1	-1,3,5-triazine					764:778	O-linked flexible 2,4,6-tris(p-formylphenoxy)-1,3,5-triazine	719:778	O-linked flexible 2,4,6-tris(p-formylphenoxy)-1,3,5-triazine	719:778	In this study, the COF prepared from O-linked flexible 2,4,6-tris(p-formylphenoxy)-1,3,5-triazine serves as an excellent absorbent for removing methylene blue from water.
31909333	7	54	theme	thermogravimetric	1179:1195	arg1	analysis					1197:1204	thermogravimetric analysis	1179:1204	thermogravimetric analysis	1179:1204	Moreover, the COF was characterized using elemental analysis, Fourier transform infrared spectroscopy, thermogravimetric analysis, scanning electron microscopy, solid-state ultraviolet-visible spectroscopy, and X-ray diffraction.
31909333	6	55	theme	pseudo-second	1049:1061	arg1	model					1069:1073	a pseudo-second order model	1047:1073	a pseudo-second order model	1047:1073	Its adsorption isotherm is consistent with the Langmuir model and its adsorption kinetics follows a pseudo-second order model.
31909333	1	56	theme	organic	129:135	arg1	COFs					149:152	COFs	149:152	COFs	149:152	The development of covalent organic frameworks (COFs) with nodes and spacers, designed to maximize their functional properties, is a challenge.
31909333	1	56	theme	organic	129:135	arg1	frameworks					137:146	covalent organic frameworks	120:146	covalent organic frameworks (COFs) with nodes and spacers	120:176	The development of covalent organic frameworks (COFs) with nodes and spacers, designed to maximize their functional properties, is a challenge.
31909333	6	57	with	consistent	976:985	arg1	model					1005:1009	the Langmuir model	992:1009	the Langmuir model	992:1009	Its adsorption isotherm is consistent with the Langmuir model and its adsorption kinetics follows a pseudo-second order model.
31909333	1	58	theme	functional	206:215	arg1	properties					217:226	their functional properties	200:226	their functional properties	200:226	The development of covalent organic frameworks (COFs) with nodes and spacers, designed to maximize their functional properties, is a challenge.
31909333	3	59	link	carbon-linked	502:514	arg1	nodes					525:529	flat, rigid sp2 carbon-linked triazine nodes	486:529	flat, rigid sp2 carbon-linked triazine nodes	486:529	Compared to COFs prepared from flat, rigid sp2 carbon-linked triazine nodes, the O-linked flexible tripodal triazine-based COF demonstrates several unpredictable properties such as an increase in crystallinity and cavity size.
31909333	2	60	link	1,3,5-benzene-linked	428:447	arg1	COFs					449:452	1,3,5-benzene-linked COFs	428:452	1,3,5-benzene-linked COFs	428:452	Triazines exhibit better electron affinity than benzene-based aromatic rings; therefore, structures based on 1,3,5-substituted triazine-centered nodes are more stable than those from 1,3,5-benzene-linked COFs.
31909333	4	61	theme	excellent	793:801	arg1	COF					701:703	the COF	697:703	the COF prepared from O-linked flexible 2,4,6-tris(p-formylphenoxy)-1,3,5-triazine	697:778	In this study, the COF prepared from O-linked flexible 2,4,6-tris(p-formylphenoxy)-1,3,5-triazine serves as an excellent absorbent for removing methylene blue from water.
31909333	4	61	theme	excellent	793:801	arg1	absorbent					803:811	an excellent absorbent	790:811	an excellent absorbent for removing methylene blue from water	790:850	In this study, the COF prepared from O-linked flexible 2,4,6-tris(p-formylphenoxy)-1,3,5-triazine serves as an excellent absorbent for removing methylene blue from water.
31909333	1	62	theme	frameworks	137:146	arg1	challenge					234:242	a challenge	232:242	a challenge	232:242	The development of covalent organic frameworks (COFs) with nodes and spacers, designed to maximize their functional properties, is a challenge.
31909333	1	62	theme	frameworks	137:146	arg1	development					105:115	The development	101:115	The development	101:115	The development of covalent organic frameworks (COFs) with nodes and spacers, designed to maximize their functional properties, is a challenge.
31909333	4	63	theme	p-formylphenoxy	748:762	arg1	-1,3,5-triazine					764:778	O-linked flexible 2,4,6-tris(p-formylphenoxy)-1,3,5-triazine	719:778	O-linked flexible 2,4,6-tris(p-formylphenoxy)-1,3,5-triazine	719:778	In this study, the COF prepared from O-linked flexible 2,4,6-tris(p-formylphenoxy)-1,3,5-triazine serves as an excellent absorbent for removing methylene blue from water.
31909333	5	64	from	water	906:910	arg1	stable					896:901	stable	896:901	stable	896:901	Our results demonstrate that COF is highly stable in water and functions as a robust adsorbent.
31909333	2	65	theme	triazine-centered	372:388	arg1	nodes					390:394	1,3,5-substituted triazine-centered nodes	354:394	1,3,5-substituted triazine-centered nodes	354:394	Triazines exhibit better electron affinity than benzene-based aromatic rings; therefore, structures based on 1,3,5-substituted triazine-centered nodes are more stable than those from 1,3,5-benzene-linked COFs.
31909333	0	66	theme	Porous	63:68	arg1	Adsorbents					70:79	Porous Adsorbents	63:79	Porous Adsorbents for Dye Pollutants	63:98	Crystalline Covalent Organic Frameworks from Triazine Nodes as Porous Adsorbents for Dye Pollutants.
31909333	3	67	dep	flat	486:489	arg1	rigid					492:496	rigid	492:496	rigid	492:496	Compared to COFs prepared from flat, rigid sp2 carbon-linked triazine nodes, the O-linked flexible tripodal triazine-based COF demonstrates several unpredictable properties such as an increase in crystallinity and cavity size.
31909333	2	68	dep	exhibit	255:261	arg1	stable					405:410	stable	405:410	stable	405:410	Triazines exhibit better electron affinity than benzene-based aromatic rings; therefore, structures based on 1,3,5-substituted triazine-centered nodes are more stable than those from 1,3,5-benzene-linked COFs.
31909333	4	69	theme	flexible	728:735	arg1	-1,3,5-triazine					764:778	O-linked flexible 2,4,6-tris(p-formylphenoxy)-1,3,5-triazine	719:778	O-linked flexible 2,4,6-tris(p-formylphenoxy)-1,3,5-triazine	719:778	In this study, the COF prepared from O-linked flexible 2,4,6-tris(p-formylphenoxy)-1,3,5-triazine serves as an excellent absorbent for removing methylene blue from water.
29642144	0	0	theme	N-linked	82:89	arg1	glycosylation					91:103	N-linked glycosylation	82:103	N-linked glycosylation	82:103	Lectin microarray and mass spectrometric analysis of hepatitis C proteins reveals N-linked glycosylation.
29642144	6	1	from	N-glycans	894:902	arg1	proteins					911:918	HCV proteins	907:918	HCV proteins	907:918	The N-glycans on HCV proteins were analyzed by lectin microarray and mass spectrometry.
29642144	5	2	theme	HCV	872:874	arg1	protein					881:887	the HCV core protein	868:887	the HCV core protein	868:887	HCVcc particles were found mainly in fractions 6 to 8, as determined by quantitative polymerase chain reaction (qPCR) analysis for HCV RNA and ELISA of the HCV core protein.
29642144	7	3	theme	major	1095:1099	arg1	glycoforms					1105:1114	the major HCV glycoforms	1091:1114	the major HCV glycoforms	1091:1114	We identified that 32 of 37 lectins displayed the positive binding signals and 16 types of N-glycoforms of which the major HCV glycoforms were high mannose-type N-linked oligosaccharides, hybrid N-glycans, and fucosylated N-glycans.
29642144	7	3	theme	major	1095:1099	arg1	oligosaccharides					1148:1163	high mannose-type N-linked oligosaccharides	1121:1163	high mannose-type N-linked oligosaccharides	1121:1163	We identified that 32 of 37 lectins displayed the positive binding signals and 16 types of N-glycoforms of which the major HCV glycoforms were high mannose-type N-linked oligosaccharides, hybrid N-glycans, and fucosylated N-glycans.
29642144	6	4	theme	mass	959:962	arg1	spectrometry					964:975	mass spectrometry	959:975	mass spectrometry	959:975	The N-glycans on HCV proteins were analyzed by lectin microarray and mass spectrometry.
29642144	7	5	dep	signals	1045:1051	arg1	the					1024:1026	the	1024:1026	the	1024:1026	We identified that 32 of 37 lectins displayed the positive binding signals and 16 types of N-glycoforms of which the major HCV glycoforms were high mannose-type N-linked oligosaccharides, hybrid N-glycans, and fucosylated N-glycans.
29642144	3	6	theme	sucrose	517:523	arg1	separation					534:543	sucrose gradient separation	517:543	sucrose gradient separation	517:543	Twelve fractions were collected from the top and analyzed for viral infectivity and HCV RNA content after sucrose gradient separation.
29642144	7	7	theme	N-glycoforms	1069:1080	arg1	types					1060:1064	16 types	1057:1064	16 types of N-glycoforms	1057:1080	We identified that 32 of 37 lectins displayed the positive binding signals and 16 types of N-glycoforms of which the major HCV glycoforms were high mannose-type N-linked oligosaccharides, hybrid N-glycans, and fucosylated N-glycans.
29642144	7	7	theme	N-glycoforms	1069:1080	arg1	signals					1045:1051	positive binding signals	1028:1051	positive binding signals	1028:1051	We identified that 32 of 37 lectins displayed the positive binding signals and 16 types of N-glycoforms of which the major HCV glycoforms were high mannose-type N-linked oligosaccharides, hybrid N-glycans, and fucosylated N-glycans.
29642144	3	8	theme	gradient	525:532	arg1	separation					534:543	sucrose gradient separation	517:543	sucrose gradient separation	517:543	Twelve fractions were collected from the top and analyzed for viral infectivity and HCV RNA content after sucrose gradient separation.
29642144	4	9	theme	continuous	610:619	arg1	gradient					640:647	a continuous 10% to 60% sucrose gradient	608:647	a continuous 10% to 60% sucrose gradient	608:647	HCV RNA and proteins were separated by ultracentrifugation in a continuous 10% to 60% sucrose gradient to purify viral particles based on their sedimentation velocities.
29642144	7	10	theme	hybrid	1166:1171	arg1	N-glycans					1173:1181	hybrid N-glycans	1166:1181	hybrid N-glycans	1166:1181	We identified that 32 of 37 lectins displayed the positive binding signals and 16 types of N-glycoforms of which the major HCV glycoforms were high mannose-type N-linked oligosaccharides, hybrid N-glycans, and fucosylated N-glycans.
29642144	7	10	theme	hybrid	1166:1171	arg1	oligosaccharides					1148:1163	high mannose-type N-linked oligosaccharides	1121:1163	high mannose-type N-linked oligosaccharides	1121:1163	We identified that 32 of 37 lectins displayed the positive binding signals and 16 types of N-glycoforms of which the major HCV glycoforms were high mannose-type N-linked oligosaccharides, hybrid N-glycans, and fucosylated N-glycans.
29642144	1	11	gly	glycosylation	189:201	arg1	particles					239:247	hepatitis C virus (HCV) particles	215:247	hepatitis C virus (HCV) particles	215:247	We used lectin microarray and mass spectrometric analysis to identify the N-linked glycosylation patterns of hepatitis C virus (HCV) particles.
29642144	8	12	theme	previous	1326:1333	arg1	findings					1335:1342	previous findings	1326:1342	previous findings of glycan-HCV protein interactions	1326:1377	Our study provided new detailed information regarding the majority of the glycan-protein profile, complementing to previous findings of glycan-HCV protein interactions.
29642144	3	13	theme	HCV	495:497	arg1	content					503:509	HCV RNA content	495:509	HCV RNA content	495:509	Twelve fractions were collected from the top and analyzed for viral infectivity and HCV RNA content after sucrose gradient separation.
29642144	1	14	theme	lectin	114:119	arg1	microarray					121:130	lectin microarray and mass spectrometric analysis	114:162	microarray	121:130	We used lectin microarray and mass spectrometric analysis to identify the N-linked glycosylation patterns of hepatitis C virus (HCV) particles.
29642144	7	15	theme	types	1060:1064	arg1	glycoforms					1105:1114	the major HCV glycoforms	1091:1114	the major HCV glycoforms	1091:1114	We identified that 32 of 37 lectins displayed the positive binding signals and 16 types of N-glycoforms of which the major HCV glycoforms were high mannose-type N-linked oligosaccharides, hybrid N-glycans, and fucosylated N-glycans.
29642144	7	15	theme	types	1060:1064	arg1	N-glycans					1173:1181	hybrid N-glycans	1166:1181	hybrid N-glycans	1166:1181	We identified that 32 of 37 lectins displayed the positive binding signals and 16 types of N-glycoforms of which the major HCV glycoforms were high mannose-type N-linked oligosaccharides, hybrid N-glycans, and fucosylated N-glycans.
29642144	7	15	theme	types	1060:1064	arg1	N-glycans					1200:1208	fucosylated N-glycans	1188:1208	fucosylated N-glycans	1188:1208	We identified that 32 of 37 lectins displayed the positive binding signals and 16 types of N-glycoforms of which the major HCV glycoforms were high mannose-type N-linked oligosaccharides, hybrid N-glycans, and fucosylated N-glycans.
29642144	7	15	theme	types	1060:1064	arg1	oligosaccharides					1148:1163	high mannose-type N-linked oligosaccharides	1121:1163	high mannose-type N-linked oligosaccharides	1121:1163	We identified that 32 of 37 lectins displayed the positive binding signals and 16 types of N-glycoforms of which the major HCV glycoforms were high mannose-type N-linked oligosaccharides, hybrid N-glycans, and fucosylated N-glycans.
29642144	8	16	theme	detailed	1234:1241	arg1	information					1243:1253	new detailed information	1230:1253	new detailed information regarding the majority of the glycan-protein profile	1230:1306	Our study provided new detailed information regarding the majority of the glycan-protein profile, complementing to previous findings of glycan-HCV protein interactions.
29642144	8	17	theme	glycan-HCV	1347:1356	arg1	interactions					1366:1377	glycan-HCV protein interactions	1347:1377	glycan-HCV protein interactions	1347:1377	Our study provided new detailed information regarding the majority of the glycan-protein profile, complementing to previous findings of glycan-HCV protein interactions.
29642144	1	18	theme	glycosylation	189:201	arg1	patterns					203:210	the N-linked glycosylation patterns	176:210	the N-linked glycosylation patterns of hepatitis C virus (HCV) particles	176:247	We used lectin microarray and mass spectrometric analysis to identify the N-linked glycosylation patterns of hepatitis C virus (HCV) particles.
29642144	4	19	theme	viral	659:663	arg1	particles					665:673	viral particles	659:673	viral particles based on their sedimentation velocities	659:713	HCV RNA and proteins were separated by ultracentrifugation in a continuous 10% to 60% sucrose gradient to purify viral particles based on their sedimentation velocities.
29642144	7	20	theme	HCV	1101:1103	arg1	glycoforms					1105:1114	the major HCV glycoforms	1091:1114	the major HCV glycoforms	1091:1114	We identified that 32 of 37 lectins displayed the positive binding signals and 16 types of N-glycoforms of which the major HCV glycoforms were high mannose-type N-linked oligosaccharides, hybrid N-glycans, and fucosylated N-glycans.
29642144	7	20	theme	HCV	1101:1103	arg1	oligosaccharides					1148:1163	high mannose-type N-linked oligosaccharides	1121:1163	high mannose-type N-linked oligosaccharides	1121:1163	We identified that 32 of 37 lectins displayed the positive binding signals and 16 types of N-glycoforms of which the major HCV glycoforms were high mannose-type N-linked oligosaccharides, hybrid N-glycans, and fucosylated N-glycans.
29642144	5	21	theme	core	876:879	arg1	protein					881:887	the HCV core protein	868:887	the HCV core protein	868:887	HCVcc particles were found mainly in fractions 6 to 8, as determined by quantitative polymerase chain reaction (qPCR) analysis for HCV RNA and ELISA of the HCV core protein.
29642144	7	22	theme	signals	1045:1051	arg1	glycoforms					1105:1114	the major HCV glycoforms	1091:1114	the major HCV glycoforms	1091:1114	We identified that 32 of 37 lectins displayed the positive binding signals and 16 types of N-glycoforms of which the major HCV glycoforms were high mannose-type N-linked oligosaccharides, hybrid N-glycans, and fucosylated N-glycans.
29642144	7	22	theme	signals	1045:1051	arg1	N-glycans					1173:1181	hybrid N-glycans	1166:1181	hybrid N-glycans	1166:1181	We identified that 32 of 37 lectins displayed the positive binding signals and 16 types of N-glycoforms of which the major HCV glycoforms were high mannose-type N-linked oligosaccharides, hybrid N-glycans, and fucosylated N-glycans.
29642144	7	22	theme	signals	1045:1051	arg1	N-glycans					1200:1208	fucosylated N-glycans	1188:1208	fucosylated N-glycans	1188:1208	We identified that 32 of 37 lectins displayed the positive binding signals and 16 types of N-glycoforms of which the major HCV glycoforms were high mannose-type N-linked oligosaccharides, hybrid N-glycans, and fucosylated N-glycans.
29642144	7	22	theme	signals	1045:1051	arg1	oligosaccharides					1148:1163	high mannose-type N-linked oligosaccharides	1121:1163	high mannose-type N-linked oligosaccharides	1121:1163	We identified that 32 of 37 lectins displayed the positive binding signals and 16 types of N-glycoforms of which the major HCV glycoforms were high mannose-type N-linked oligosaccharides, hybrid N-glycans, and fucosylated N-glycans.
29642144	2	23	theme	cell	272:275	arg1	HCVcc					286:290	HCVcc	286:290	HCVcc	286:290	HCV J6/JFH-1 chimeric cell culture (HCVcc) in the culture supernatant was concentrated and purified by ultrafiltration and sucrose gradient ultracentrifugation.
29642144	2	23	theme	cell	272:275	arg1	culture					277:283	HCV J6/JFH-1 chimeric cell culture	250:283	HCV J6/JFH-1 chimeric cell culture (HCVcc) in the culture supernatant	250:318	HCV J6/JFH-1 chimeric cell culture (HCVcc) in the culture supernatant was concentrated and purified by ultrafiltration and sucrose gradient ultracentrifugation.
29642144	3	24	theme	RNA	499:501	arg1	content					503:509	HCV RNA content	495:509	HCV RNA content	495:509	Twelve fractions were collected from the top and analyzed for viral infectivity and HCV RNA content after sucrose gradient separation.
29642144	1	25	theme	N-linked	180:187	arg1	patterns					203:210	the N-linked glycosylation patterns	176:210	the N-linked glycosylation patterns of hepatitis C virus (HCV) particles	176:247	We used lectin microarray and mass spectrometric analysis to identify the N-linked glycosylation patterns of hepatitis C virus (HCV) particles.
29642144	5	26	theme	polymerase	801:810	arg1	qPCR					828:831	qPCR	828:831	qPCR	828:831	HCVcc particles were found mainly in fractions 6 to 8, as determined by quantitative polymerase chain reaction (qPCR) analysis for HCV RNA and ELISA of the HCV core protein.
29642144	5	26	theme	polymerase	801:810	arg1	reaction					818:825	quantitative polymerase chain reaction	788:825	quantitative polymerase chain reaction (qPCR) analysis for HCV RNA and ELISA of the HCV core protein	788:887	HCVcc particles were found mainly in fractions 6 to 8, as determined by quantitative polymerase chain reaction (qPCR) analysis for HCV RNA and ELISA of the HCV core protein.
29642144	7	27	theme	fucosylated	1188:1198	arg1	N-glycans					1200:1208	fucosylated N-glycans	1188:1208	fucosylated N-glycans	1188:1208	We identified that 32 of 37 lectins displayed the positive binding signals and 16 types of N-glycoforms of which the major HCV glycoforms were high mannose-type N-linked oligosaccharides, hybrid N-glycans, and fucosylated N-glycans.
29642144	7	27	theme	fucosylated	1188:1198	arg1	oligosaccharides					1148:1163	high mannose-type N-linked oligosaccharides	1121:1163	high mannose-type N-linked oligosaccharides	1121:1163	We identified that 32 of 37 lectins displayed the positive binding signals and 16 types of N-glycoforms of which the major HCV glycoforms were high mannose-type N-linked oligosaccharides, hybrid N-glycans, and fucosylated N-glycans.
29642144	2	28	theme	culture	300:306	arg1	supernatant					308:318	the culture supernatant	296:318	the culture supernatant	296:318	HCV J6/JFH-1 chimeric cell culture (HCVcc) in the culture supernatant was concentrated and purified by ultrafiltration and sucrose gradient ultracentrifugation.
29642144	2	29	theme	gradient	381:388	arg1	ultracentrifugation					390:408	sucrose gradient ultracentrifugation	373:408	sucrose gradient ultracentrifugation	373:408	HCV J6/JFH-1 chimeric cell culture (HCVcc) in the culture supernatant was concentrated and purified by ultrafiltration and sucrose gradient ultracentrifugation.
29642144	0	30	theme	Lectin	0:5	arg1	microarray					7:16	Lectin microarray and mass spectrometric analysis	0:48	microarray	7:16	Lectin microarray and mass spectrometric analysis of hepatitis C proteins reveals N-linked glycosylation.
29642144	1	31	link	N-linked	180:187	arg1	patterns					203:210	the N-linked glycosylation patterns	176:210	the N-linked glycosylation patterns of hepatitis C virus (HCV) particles	176:247	We used lectin microarray and mass spectrometric analysis to identify the N-linked glycosylation patterns of hepatitis C virus (HCV) particles.
29642144	5	32	theme	HCV	847:849	arg1	RNA					851:853	HCV RNA	847:853	HCV RNA	847:853	HCVcc particles were found mainly in fractions 6 to 8, as determined by quantitative polymerase chain reaction (qPCR) analysis for HCV RNA and ELISA of the HCV core protein.
29642144	8	33	theme	protein	1358:1364	arg1	interactions					1366:1377	glycan-HCV protein interactions	1347:1377	glycan-HCV protein interactions	1347:1377	Our study provided new detailed information regarding the majority of the glycan-protein profile, complementing to previous findings of glycan-HCV protein interactions.
29642144	2	34	theme	sucrose	373:379	arg1	ultracentrifugation					390:408	sucrose gradient ultracentrifugation	373:408	sucrose gradient ultracentrifugation	373:408	HCV J6/JFH-1 chimeric cell culture (HCVcc) in the culture supernatant was concentrated and purified by ultrafiltration and sucrose gradient ultracentrifugation.
29642144	0	35	theme	mass	22:25	arg1	analysis					41:48	Lectin microarray and mass spectrometric analysis	0:48	analysis	41:48	Lectin microarray and mass spectrometric analysis of hepatitis C proteins reveals N-linked glycosylation.
29642144	5	36	theme	chain	812:816	arg1	qPCR					828:831	qPCR	828:831	qPCR	828:831	HCVcc particles were found mainly in fractions 6 to 8, as determined by quantitative polymerase chain reaction (qPCR) analysis for HCV RNA and ELISA of the HCV core protein.
29642144	5	36	theme	chain	812:816	arg1	reaction					818:825	quantitative polymerase chain reaction	788:825	quantitative polymerase chain reaction (qPCR) analysis for HCV RNA and ELISA of the HCV core protein	788:887	HCVcc particles were found mainly in fractions 6 to 8, as determined by quantitative polymerase chain reaction (qPCR) analysis for HCV RNA and ELISA of the HCV core protein.
29642144	7	37	theme	binding	1037:1043	arg1	signals					1045:1051	positive binding signals	1028:1051	positive binding signals	1028:1051	We identified that 32 of 37 lectins displayed the positive binding signals and 16 types of N-glycoforms of which the major HCV glycoforms were high mannose-type N-linked oligosaccharides, hybrid N-glycans, and fucosylated N-glycans.
29642144	7	38	gly	fucosylated	1188:1198	arg1	N-glycans					1200:1208	fucosylated N-glycans	1188:1208	fucosylated N-glycans	1188:1208	We identified that 32 of 37 lectins displayed the positive binding signals and 16 types of N-glycoforms of which the major HCV glycoforms were high mannose-type N-linked oligosaccharides, hybrid N-glycans, and fucosylated N-glycans.
29642144	7	38	gly	fucosylated	1188:1198	arg1	oligosaccharides					1148:1163	high mannose-type N-linked oligosaccharides	1121:1163	high mannose-type N-linked oligosaccharides	1121:1163	We identified that 32 of 37 lectins displayed the positive binding signals and 16 types of N-glycoforms of which the major HCV glycoforms were high mannose-type N-linked oligosaccharides, hybrid N-glycans, and fucosylated N-glycans.
29642144	3	39	theme	viral	473:477	arg1	infectivity					479:489	viral infectivity	473:489	viral infectivity	473:489	Twelve fractions were collected from the top and analyzed for viral infectivity and HCV RNA content after sucrose gradient separation.
29642144	5	40	located	found	737:741	arg2	particles					722:730	HCVcc particles	716:730	HCVcc particles	716:730	HCVcc particles were found mainly in fractions 6 to 8, as determined by quantitative polymerase chain reaction (qPCR) analysis for HCV RNA and ELISA of the HCV core protein.
29642144	5	40	located	found	737:741	arg1	fractions					753:761	fractions 6 to 8	753:768	fractions 6 to 8	753:768	HCVcc particles were found mainly in fractions 6 to 8, as determined by quantitative polymerase chain reaction (qPCR) analysis for HCV RNA and ELISA of the HCV core protein.
29642144	5	41	theme	HCVcc	716:720	arg1	particles					722:730	HCVcc particles	716:730	HCVcc particles	716:730	HCVcc particles were found mainly in fractions 6 to 8, as determined by quantitative polymerase chain reaction (qPCR) analysis for HCV RNA and ELISA of the HCV core protein.
29642144	4	42	dep	continuous	610:619	arg1	%					630:630	10% to 60%	621:630	10% to 60%	621:630	HCV RNA and proteins were separated by ultracentrifugation in a continuous 10% to 60% sucrose gradient to purify viral particles based on their sedimentation velocities.
29642144	4	43	theme	sedimentation	690:702	arg1	velocities					704:713	their sedimentation velocities	684:713	their sedimentation velocities	684:713	HCV RNA and proteins were separated by ultracentrifugation in a continuous 10% to 60% sucrose gradient to purify viral particles based on their sedimentation velocities.
29642144	8	44	theme	interactions	1366:1377	arg1	findings					1335:1342	previous findings	1326:1342	previous findings of glycan-HCV protein interactions	1326:1377	Our study provided new detailed information regarding the majority of the glycan-protein profile, complementing to previous findings of glycan-HCV protein interactions.
29642144	1	45	theme	hepatitis	215:223	arg1	HCV					234:236	HCV	234:236	HCV	234:236	We used lectin microarray and mass spectrometric analysis to identify the N-linked glycosylation patterns of hepatitis C virus (HCV) particles.
29642144	1	45	theme	hepatitis	215:223	arg1	virus					227:231	hepatitis C virus	215:231	hepatitis C virus (HCV) particles	215:247	We used lectin microarray and mass spectrometric analysis to identify the N-linked glycosylation patterns of hepatitis C virus (HCV) particles.
29642144	1	46	theme	C	225:225	arg1	HCV					234:236	HCV	234:236	HCV	234:236	We used lectin microarray and mass spectrometric analysis to identify the N-linked glycosylation patterns of hepatitis C virus (HCV) particles.
29642144	1	46	theme	C	225:225	arg1	virus					227:231	hepatitis C virus	215:231	hepatitis C virus (HCV) particles	215:247	We used lectin microarray and mass spectrometric analysis to identify the N-linked glycosylation patterns of hepatitis C virus (HCV) particles.
29642144	0	47	theme	spectrometric	27:39	arg1	analysis					41:48	Lectin microarray and mass spectrometric analysis	0:48	analysis	41:48	Lectin microarray and mass spectrometric analysis of hepatitis C proteins reveals N-linked glycosylation.
29642144	4	48	theme	HCV	546:548	arg1	RNA					550:552	HCV RNA	546:552	HCV RNA	546:552	HCV RNA and proteins were separated by ultracentrifugation in a continuous 10% to 60% sucrose gradient to purify viral particles based on their sedimentation velocities.
29642144	7	49	theme	positive	1028:1035	arg1	signals					1045:1051	positive binding signals	1028:1051	positive binding signals	1028:1051	We identified that 32 of 37 lectins displayed the positive binding signals and 16 types of N-glycoforms of which the major HCV glycoforms were high mannose-type N-linked oligosaccharides, hybrid N-glycans, and fucosylated N-glycans.
29642144	8	50	theme	profile	1300:1306	arg1	majority					1269:1276	the majority	1265:1276	the majority of the glycan-protein profile	1265:1306	Our study provided new detailed information regarding the majority of the glycan-protein profile, complementing to previous findings of glycan-HCV protein interactions.
29642144	5	51	theme	reaction	818:825	arg1	analysis					834:841	quantitative polymerase chain reaction (qPCR) analysis	788:841	quantitative polymerase chain reaction (qPCR) analysis for HCV RNA and ELISA of the HCV core protein	788:887	HCVcc particles were found mainly in fractions 6 to 8, as determined by quantitative polymerase chain reaction (qPCR) analysis for HCV RNA and ELISA of the HCV core protein.
29642144	0	52	link	N-linked	82:89	arg1	glycosylation					91:103	N-linked glycosylation	82:103	N-linked glycosylation	82:103	Lectin microarray and mass spectrometric analysis of hepatitis C proteins reveals N-linked glycosylation.
29642144	1	53	theme	virus	227:231	arg1	particles					239:247	hepatitis C virus (HCV) particles	215:247	hepatitis C virus (HCV) particles	215:247	We used lectin microarray and mass spectrometric analysis to identify the N-linked glycosylation patterns of hepatitis C virus (HCV) particles.
29642144	0	54	theme	hepatitis	53:61	arg1	proteins					65:72	hepatitis C proteins	53:72	hepatitis C proteins	53:72	Lectin microarray and mass spectrometric analysis of hepatitis C proteins reveals N-linked glycosylation.
29642144	7	55	theme	lectins	1006:1012	arg1	lectins					1006:1012	37 lectins	1003:1012	37 lectins	1003:1012	We identified that 32 of 37 lectins displayed the positive binding signals and 16 types of N-glycoforms of which the major HCV glycoforms were high mannose-type N-linked oligosaccharides, hybrid N-glycans, and fucosylated N-glycans.
29642144	7	55	theme	lectins	1006:1012	arg1	32					997:998	32	997:998	32	997:998	We identified that 32 of 37 lectins displayed the positive binding signals and 16 types of N-glycoforms of which the major HCV glycoforms were high mannose-type N-linked oligosaccharides, hybrid N-glycans, and fucosylated N-glycans.
29642144	6	56	theme	lectin	937:942	arg1	microarray					944:953	lectin microarray	937:953	lectin microarray	937:953	The N-glycans on HCV proteins were analyzed by lectin microarray and mass spectrometry.
29642144	7	57	link	N-linked	1139:1146	arg1	glycoforms					1105:1114	the major HCV glycoforms	1091:1114	the major HCV glycoforms	1091:1114	We identified that 32 of 37 lectins displayed the positive binding signals and 16 types of N-glycoforms of which the major HCV glycoforms were high mannose-type N-linked oligosaccharides, hybrid N-glycans, and fucosylated N-glycans.
29642144	7	57	link	N-linked	1139:1146	arg1	N-glycans					1173:1181	hybrid N-glycans	1166:1181	hybrid N-glycans	1166:1181	We identified that 32 of 37 lectins displayed the positive binding signals and 16 types of N-glycoforms of which the major HCV glycoforms were high mannose-type N-linked oligosaccharides, hybrid N-glycans, and fucosylated N-glycans.
29642144	7	57	link	N-linked	1139:1146	arg1	N-glycans					1200:1208	fucosylated N-glycans	1188:1208	fucosylated N-glycans	1188:1208	We identified that 32 of 37 lectins displayed the positive binding signals and 16 types of N-glycoforms of which the major HCV glycoforms were high mannose-type N-linked oligosaccharides, hybrid N-glycans, and fucosylated N-glycans.
29642144	7	57	link	N-linked	1139:1146	arg1	oligosaccharides					1148:1163	high mannose-type N-linked oligosaccharides	1121:1163	high mannose-type N-linked oligosaccharides	1121:1163	We identified that 32 of 37 lectins displayed the positive binding signals and 16 types of N-glycoforms of which the major HCV glycoforms were high mannose-type N-linked oligosaccharides, hybrid N-glycans, and fucosylated N-glycans.
29642144	1	58	used	used	109:112	arg2	We					106:107	We	106:107	We	106:107	We used lectin microarray and mass spectrometric analysis to identify the N-linked glycosylation patterns of hepatitis C virus (HCV) particles.
29642144	5	59	theme	quantitative	788:799	arg1	qPCR					828:831	qPCR	828:831	qPCR	828:831	HCVcc particles were found mainly in fractions 6 to 8, as determined by quantitative polymerase chain reaction (qPCR) analysis for HCV RNA and ELISA of the HCV core protein.
29642144	5	59	theme	quantitative	788:799	arg1	reaction					818:825	quantitative polymerase chain reaction	788:825	quantitative polymerase chain reaction (qPCR) analysis for HCV RNA and ELISA of the HCV core protein	788:887	HCVcc particles were found mainly in fractions 6 to 8, as determined by quantitative polymerase chain reaction (qPCR) analysis for HCV RNA and ELISA of the HCV core protein.
29642144	6	60	theme	HCV	907:909	arg1	proteins					911:918	HCV proteins	907:918	HCV proteins	907:918	The N-glycans on HCV proteins were analyzed by lectin microarray and mass spectrometry.
29642144	5	61	dep	fractions	753:761	arg1	to					765:766	to	765:766	to	765:766	HCVcc particles were found mainly in fractions 6 to 8, as determined by quantitative polymerase chain reaction (qPCR) analysis for HCV RNA and ELISA of the HCV core protein.
29642144	4	62	dep	%	630:630	arg1	to					625:626	to	625:626	to	625:626	HCV RNA and proteins were separated by ultracentrifugation in a continuous 10% to 60% sucrose gradient to purify viral particles based on their sedimentation velocities.
29642144	8	63	theme	new	1230:1232	arg1	information					1243:1253	new detailed information	1230:1253	new detailed information regarding the majority of the glycan-protein profile	1230:1306	Our study provided new detailed information regarding the majority of the glycan-protein profile, complementing to previous findings of glycan-HCV protein interactions.
29642144	2	64	from	culture	277:283	arg1	supernatant					308:318	the culture supernatant	296:318	the culture supernatant	296:318	HCV J6/JFH-1 chimeric cell culture (HCVcc) in the culture supernatant was concentrated and purified by ultrafiltration and sucrose gradient ultracentrifugation.
29642144	7	65	theme	high	1121:1124	arg1	glycoforms					1105:1114	the major HCV glycoforms	1091:1114	the major HCV glycoforms	1091:1114	We identified that 32 of 37 lectins displayed the positive binding signals and 16 types of N-glycoforms of which the major HCV glycoforms were high mannose-type N-linked oligosaccharides, hybrid N-glycans, and fucosylated N-glycans.
29642144	7	65	theme	high	1121:1124	arg1	N-glycans					1173:1181	hybrid N-glycans	1166:1181	hybrid N-glycans	1166:1181	We identified that 32 of 37 lectins displayed the positive binding signals and 16 types of N-glycoforms of which the major HCV glycoforms were high mannose-type N-linked oligosaccharides, hybrid N-glycans, and fucosylated N-glycans.
29642144	7	65	theme	high	1121:1124	arg1	N-glycans					1200:1208	fucosylated N-glycans	1188:1208	fucosylated N-glycans	1188:1208	We identified that 32 of 37 lectins displayed the positive binding signals and 16 types of N-glycoforms of which the major HCV glycoforms were high mannose-type N-linked oligosaccharides, hybrid N-glycans, and fucosylated N-glycans.
29642144	7	65	theme	high	1121:1124	arg1	oligosaccharides					1148:1163	high mannose-type N-linked oligosaccharides	1121:1163	high mannose-type N-linked oligosaccharides	1121:1163	We identified that 32 of 37 lectins displayed the positive binding signals and 16 types of N-glycoforms of which the major HCV glycoforms were high mannose-type N-linked oligosaccharides, hybrid N-glycans, and fucosylated N-glycans.
29642144	2	66	theme	chimeric	263:270	arg1	HCVcc					286:290	HCVcc	286:290	HCVcc	286:290	HCV J6/JFH-1 chimeric cell culture (HCVcc) in the culture supernatant was concentrated and purified by ultrafiltration and sucrose gradient ultracentrifugation.
29642144	2	66	theme	chimeric	263:270	arg1	culture					277:283	HCV J6/JFH-1 chimeric cell culture	250:283	HCV J6/JFH-1 chimeric cell culture (HCVcc) in the culture supernatant	250:318	HCV J6/JFH-1 chimeric cell culture (HCVcc) in the culture supernatant was concentrated and purified by ultrafiltration and sucrose gradient ultracentrifugation.
29642144	7	67	gly	glycoforms	1105:1114	arg1	HCV					1101:1103	the major HCV glycoforms	1091:1114	the major HCV glycoforms	1091:1114	We identified that 32 of 37 lectins displayed the positive binding signals and 16 types of N-glycoforms of which the major HCV glycoforms were high mannose-type N-linked oligosaccharides, hybrid N-glycans, and fucosylated N-glycans.
29642144	8	68	theme	glycan-protein	1285:1298	arg1	profile					1300:1306	the glycan-protein profile	1281:1306	the glycan-protein profile	1281:1306	Our study provided new detailed information regarding the majority of the glycan-protein profile, complementing to previous findings of glycan-HCV protein interactions.
29642144	4	69	theme	%	623:623	arg1	%					630:630	10% to 60%	621:630	10% to 60%	621:630	HCV RNA and proteins were separated by ultracentrifugation in a continuous 10% to 60% sucrose gradient to purify viral particles based on their sedimentation velocities.
29642144	5	70	theme	protein	881:887	arg1	RNA					851:853	HCV RNA	847:853	HCV RNA	847:853	HCVcc particles were found mainly in fractions 6 to 8, as determined by quantitative polymerase chain reaction (qPCR) analysis for HCV RNA and ELISA of the HCV core protein.
29642144	5	70	theme	protein	881:887	arg1	ELISA					859:863	ELISA	859:863	ELISA	859:863	HCVcc particles were found mainly in fractions 6 to 8, as determined by quantitative polymerase chain reaction (qPCR) analysis for HCV RNA and ELISA of the HCV core protein.
29642144	2	71	theme	J6/JFH-1	254:261	arg1	HCVcc					286:290	HCVcc	286:290	HCVcc	286:290	HCV J6/JFH-1 chimeric cell culture (HCVcc) in the culture supernatant was concentrated and purified by ultrafiltration and sucrose gradient ultracentrifugation.
29642144	2	71	theme	J6/JFH-1	254:261	arg1	culture					277:283	HCV J6/JFH-1 chimeric cell culture	250:283	HCV J6/JFH-1 chimeric cell culture (HCVcc) in the culture supernatant	250:318	HCV J6/JFH-1 chimeric cell culture (HCVcc) in the culture supernatant was concentrated and purified by ultrafiltration and sucrose gradient ultracentrifugation.
29642144	1	72	theme	mass	136:139	arg1	analysis					155:162	lectin microarray and mass spectrometric analysis	114:162	analysis	155:162	We used lectin microarray and mass spectrometric analysis to identify the N-linked glycosylation patterns of hepatitis C virus (HCV) particles.
29642144	0	73	theme	proteins	65:72	arg1	analysis					41:48	Lectin microarray and mass spectrometric analysis	0:48	analysis	41:48	Lectin microarray and mass spectrometric analysis of hepatitis C proteins reveals N-linked glycosylation.
29642144	0	73	theme	proteins	65:72	arg1	microarray					7:16	Lectin microarray and mass spectrometric analysis	0:48	microarray	7:16	Lectin microarray and mass spectrometric analysis of hepatitis C proteins reveals N-linked glycosylation.
29642144	2	74	theme	HCV	250:252	arg1	HCVcc					286:290	HCVcc	286:290	HCVcc	286:290	HCV J6/JFH-1 chimeric cell culture (HCVcc) in the culture supernatant was concentrated and purified by ultrafiltration and sucrose gradient ultracentrifugation.
29642144	2	74	theme	HCV	250:252	arg1	culture					277:283	HCV J6/JFH-1 chimeric cell culture	250:283	HCV J6/JFH-1 chimeric cell culture (HCVcc) in the culture supernatant	250:318	HCV J6/JFH-1 chimeric cell culture (HCVcc) in the culture supernatant was concentrated and purified by ultrafiltration and sucrose gradient ultracentrifugation.
29642144	4	75	theme	sucrose	632:638	arg1	gradient					640:647	a continuous 10% to 60% sucrose gradient	608:647	a continuous 10% to 60% sucrose gradient	608:647	HCV RNA and proteins were separated by ultracentrifugation in a continuous 10% to 60% sucrose gradient to purify viral particles based on their sedimentation velocities.
29642144	1	76	theme	spectrometric	141:153	arg1	analysis					155:162	lectin microarray and mass spectrometric analysis	114:162	analysis	155:162	We used lectin microarray and mass spectrometric analysis to identify the N-linked glycosylation patterns of hepatitis C virus (HCV) particles.
29642144	0	77	theme	C	63:63	arg1	proteins					65:72	hepatitis C proteins	53:72	hepatitis C proteins	53:72	Lectin microarray and mass spectrometric analysis of hepatitis C proteins reveals N-linked glycosylation.
29642144	7	78	theme	mannose-type	1126:1137	arg1	glycoforms					1105:1114	the major HCV glycoforms	1091:1114	the major HCV glycoforms	1091:1114	We identified that 32 of 37 lectins displayed the positive binding signals and 16 types of N-glycoforms of which the major HCV glycoforms were high mannose-type N-linked oligosaccharides, hybrid N-glycans, and fucosylated N-glycans.
29642144	7	78	theme	mannose-type	1126:1137	arg1	N-glycans					1173:1181	hybrid N-glycans	1166:1181	hybrid N-glycans	1166:1181	We identified that 32 of 37 lectins displayed the positive binding signals and 16 types of N-glycoforms of which the major HCV glycoforms were high mannose-type N-linked oligosaccharides, hybrid N-glycans, and fucosylated N-glycans.
29642144	7	78	theme	mannose-type	1126:1137	arg1	N-glycans					1200:1208	fucosylated N-glycans	1188:1208	fucosylated N-glycans	1188:1208	We identified that 32 of 37 lectins displayed the positive binding signals and 16 types of N-glycoforms of which the major HCV glycoforms were high mannose-type N-linked oligosaccharides, hybrid N-glycans, and fucosylated N-glycans.
29642144	7	78	theme	mannose-type	1126:1137	arg1	oligosaccharides					1148:1163	high mannose-type N-linked oligosaccharides	1121:1163	high mannose-type N-linked oligosaccharides	1121:1163	We identified that 32 of 37 lectins displayed the positive binding signals and 16 types of N-glycoforms of which the major HCV glycoforms were high mannose-type N-linked oligosaccharides, hybrid N-glycans, and fucosylated N-glycans.
29642144	7	79	theme	N-linked	1139:1146	arg1	glycoforms					1105:1114	the major HCV glycoforms	1091:1114	the major HCV glycoforms	1091:1114	We identified that 32 of 37 lectins displayed the positive binding signals and 16 types of N-glycoforms of which the major HCV glycoforms were high mannose-type N-linked oligosaccharides, hybrid N-glycans, and fucosylated N-glycans.
29642144	7	79	theme	N-linked	1139:1146	arg1	N-glycans					1173:1181	hybrid N-glycans	1166:1181	hybrid N-glycans	1166:1181	We identified that 32 of 37 lectins displayed the positive binding signals and 16 types of N-glycoforms of which the major HCV glycoforms were high mannose-type N-linked oligosaccharides, hybrid N-glycans, and fucosylated N-glycans.
29642144	7	79	theme	N-linked	1139:1146	arg1	N-glycans					1200:1208	fucosylated N-glycans	1188:1208	fucosylated N-glycans	1188:1208	We identified that 32 of 37 lectins displayed the positive binding signals and 16 types of N-glycoforms of which the major HCV glycoforms were high mannose-type N-linked oligosaccharides, hybrid N-glycans, and fucosylated N-glycans.
29642144	7	79	theme	N-linked	1139:1146	arg1	oligosaccharides					1148:1163	high mannose-type N-linked oligosaccharides	1121:1163	high mannose-type N-linked oligosaccharides	1121:1163	We identified that 32 of 37 lectins displayed the positive binding signals and 16 types of N-glycoforms of which the major HCV glycoforms were high mannose-type N-linked oligosaccharides, hybrid N-glycans, and fucosylated N-glycans.
29642144	1	80	theme	particles	239:247	arg1	patterns					203:210	the N-linked glycosylation patterns	176:210	the N-linked glycosylation patterns of hepatitis C virus (HCV) particles	176:247	We used lectin microarray and mass spectrometric analysis to identify the N-linked glycosylation patterns of hepatitis C virus (HCV) particles.
29723274	0	0	theme	glycans	106:112	arg1	chromatography					64:77	liquid chromatography	57:77	liquid chromatography of immunoglobulin N-linked glycans	57:112	Rapid chemical de-N-glycosylation and derivatization for liquid chromatography of immunoglobulin N-linked glycans.
29723274	1	1	gly	heterogeneity	197:209	arg1	glycosylation					214:226	glycosylation	214:226	glycosylation of biopharmaceuticals	214:248	Glycan analysis may result in exploitation of glycan biomarkers and evaluation of heterogeneity of glycosylation of biopharmaceuticals.
29723274	0	2	theme	N-linked	97:104	arg1	glycans					106:112	immunoglobulin N-linked glycans	82:112	immunoglobulin N-linked glycans	82:112	Rapid chemical de-N-glycosylation and derivatization for liquid chromatography of immunoglobulin N-linked glycans.
29723274	5	3	contain	containing	960:969	arg1	NeuGc					927:931	NeuGc	927:931	NeuGc	927:931	Here, we demonstrated N-linked glycan analysis using this method for a monoclonal antibody, and examined whether this method could liberate glycans without degradation from apo-transferrin containing NeuAc and NeuGc and horseradish peroxidase containing Fuc α1-3 GlcNAc at the reducing end.
29723274	5	3	contain	containing	960:969	arg2	GlcNAc					980:985	Fuc α1-3 GlcNAc	971:985	Fuc α1-3 GlcNAc	971:985	Here, we demonstrated N-linked glycan analysis using this method for a monoclonal antibody, and examined whether this method could liberate glycans without degradation from apo-transferrin containing NeuAc and NeuGc and horseradish peroxidase containing Fuc α1-3 GlcNAc at the reducing end.
29723274	3	4	theme	glycosylamines	564:577	arg1	mixture					527:533	a mixture	525:533	a mixture of oximes, free glycans, and glycosylamines	525:577	By adding hydroxylamine into alkaline de-N-glycosylation, we suppressed the degradation of released glycans and obtained a mixture of oximes, free glycans, and glycosylamines.
29723274	1	5	gly	glycosylation	214:226	arg1	biopharmaceuticals					231:248	biopharmaceuticals	231:248	biopharmaceuticals	231:248	Glycan analysis may result in exploitation of glycan biomarkers and evaluation of heterogeneity of glycosylation of biopharmaceuticals.
29723274	8	6	from	50°C	1380:1383	arg1	reaction					1310:1317	the reaction	1306:1317	the reaction at 80°C for 1 h for asialo glycoproteins from mammals and at 50°C for 1 h for sialoglycoproteins	1306:1414	Considering this balance, we proposed to carry out the reaction at 80°C for 1 h for asialo glycoproteins from mammals and at 50°C for 1 h for sialoglycoproteins.
29723274	2	7	theme	glycosyl	335:342	arg1	carboxamide					344:354	the asparagine glycosyl carboxamide	320:354	the asparagine glycosyl carboxamide of glycoproteins	320:371	For N-linked glycan analysis, we investigated alkaline hydrolysis of the asparagine glycosyl carboxamide of glycoproteins as a deglycosylation reaction.
29723274	6	8	theme	glycan	1082:1087	arg1	release					1089:1095	conventional enzymatic glycan release	1059:1095	conventional enzymatic glycan release	1059:1095	Furthermore, we compared glycan recoveries between conventional enzymatic glycan release and this method.
29723274	5	9	theme	monoclonal	788:797	arg1	antibody					799:806	a monoclonal antibody	786:806	a monoclonal antibody	786:806	Here, we demonstrated N-linked glycan analysis using this method for a monoclonal antibody, and examined whether this method could liberate glycans without degradation from apo-transferrin containing NeuAc and NeuGc and horseradish peroxidase containing Fuc α1-3 GlcNAc at the reducing end.
29723274	8	10	from	80°C	1322:1325	arg1	reaction					1310:1317	the reaction	1306:1317	the reaction at 80°C for 1 h for asialo glycoproteins from mammals and at 50°C for 1 h for sialoglycoproteins	1306:1414	Considering this balance, we proposed to carry out the reaction at 80°C for 1 h for asialo glycoproteins from mammals and at 50°C for 1 h for sialoglycoproteins.
29723274	2	11	theme	asparagine	324:333	arg1	carboxamide					344:354	the asparagine glycosyl carboxamide	320:354	the asparagine glycosyl carboxamide of glycoproteins	320:371	For N-linked glycan analysis, we investigated alkaline hydrolysis of the asparagine glycosyl carboxamide of glycoproteins as a deglycosylation reaction.
29723274	5	12	link	N-linked	739:746	arg1	analysis					755:762	N-linked glycan analysis	739:762	N-linked glycan analysis using this method for a monoclonal antibody	739:806	Here, we demonstrated N-linked glycan analysis using this method for a monoclonal antibody, and examined whether this method could liberate glycans without degradation from apo-transferrin containing NeuAc and NeuGc and horseradish peroxidase containing Fuc α1-3 GlcNAc at the reducing end.
29723274	3	13	theme	oximes	538:543	arg1	mixture					527:533	a mixture	525:533	a mixture of oximes, free glycans, and glycosylamines	525:577	By adding hydroxylamine into alkaline de-N-glycosylation, we suppressed the degradation of released glycans and obtained a mixture of oximes, free glycans, and glycosylamines.
29723274	6	14	theme	enzymatic	1072:1080	arg1	release					1089:1095	conventional enzymatic glycan release	1059:1095	conventional enzymatic glycan release	1059:1095	Furthermore, we compared glycan recoveries between conventional enzymatic glycan release and this method.
29723274	1	15	theme	heterogeneity	197:209	arg1	exploitation					145:156	exploitation	145:156	exploitation of glycan biomarkers	145:177	Glycan analysis may result in exploitation of glycan biomarkers and evaluation of heterogeneity of glycosylation of biopharmaceuticals.
29723274	1	15	theme	heterogeneity	197:209	arg1	evaluation					183:192	evaluation	183:192	evaluation of heterogeneity of glycosylation of biopharmaceuticals	183:248	Glycan analysis may result in exploitation of glycan biomarkers and evaluation of heterogeneity of glycosylation of biopharmaceuticals.
29723274	0	16	theme	chemical	6:13	arg1	de-N-glycosylation					15:32	Rapid chemical de-N-glycosylation	0:32	Rapid chemical de-N-glycosylation	0:32	Rapid chemical de-N-glycosylation and derivatization for liquid chromatography of immunoglobulin N-linked glycans.
29723274	8	17	gly	sialoglycoproteins	1397:1414	arg1	sialoglycoproteins					1397:1414	sialoglycoproteins	1397:1414	sialoglycoproteins	1397:1414	Considering this balance, we proposed to carry out the reaction at 80°C for 1 h for asialo glycoproteins from mammals and at 50°C for 1 h for sialoglycoproteins.
29723274	0	18	theme	immunoglobulin	82:95	arg1	glycans					106:112	immunoglobulin N-linked glycans	82:112	immunoglobulin N-linked glycans	82:112	Rapid chemical de-N-glycosylation and derivatization for liquid chromatography of immunoglobulin N-linked glycans.
29723274	3	19	theme	released	495:502	arg1	glycans					504:510	released glycans	495:510	released glycans	495:510	By adding hydroxylamine into alkaline de-N-glycosylation, we suppressed the degradation of released glycans and obtained a mixture of oximes, free glycans, and glycosylamines.
29723274	0	20	theme	Rapid	0:4	arg1	de-N-glycosylation					15:32	Rapid chemical de-N-glycosylation	0:32	Rapid chemical de-N-glycosylation	0:32	Rapid chemical de-N-glycosylation and derivatization for liquid chromatography of immunoglobulin N-linked glycans.
29723274	6	21	theme	conventional	1059:1070	arg1	release					1089:1095	conventional enzymatic glycan release	1059:1095	conventional enzymatic glycan release	1059:1095	Furthermore, we compared glycan recoveries between conventional enzymatic glycan release and this method.
29723274	1	22	theme	Glycan	115:120	arg1	analysis					122:129	Glycan analysis	115:129	Glycan analysis	115:129	Glycan analysis may result in exploitation of glycan biomarkers and evaluation of heterogeneity of glycosylation of biopharmaceuticals.
29723274	8	23	theme	asialo	1339:1344	arg1	glycoproteins					1346:1358	asialo glycoproteins	1339:1358	asialo glycoproteins from mammals	1339:1371	Considering this balance, we proposed to carry out the reaction at 80°C for 1 h for asialo glycoproteins from mammals and at 50°C for 1 h for sialoglycoproteins.
29723274	1	24	theme	glycosylation	214:226	arg1	heterogeneity					197:209	heterogeneity	197:209	heterogeneity of glycosylation of biopharmaceuticals	197:248	Glycan analysis may result in exploitation of glycan biomarkers and evaluation of heterogeneity of glycosylation of biopharmaceuticals.
29723274	3	25	theme	alkaline	433:440	arg1	de-N-glycosylation					442:459	alkaline de-N-glycosylation	433:459	alkaline de-N-glycosylation	433:459	By adding hydroxylamine into alkaline de-N-glycosylation, we suppressed the degradation of released glycans and obtained a mixture of oximes, free glycans, and glycosylamines.
29723274	5	26	contain	containing	906:915	arg2	NeuGc					927:931	NeuGc	927:931	NeuGc	927:931	Here, we demonstrated N-linked glycan analysis using this method for a monoclonal antibody, and examined whether this method could liberate glycans without degradation from apo-transferrin containing NeuAc and NeuGc and horseradish peroxidase containing Fuc α1-3 GlcNAc at the reducing end.
29723274	5	26	contain	containing	906:915	arg2	NeuAc					917:921	NeuAc	917:921	NeuAc	917:921	Here, we demonstrated N-linked glycan analysis using this method for a monoclonal antibody, and examined whether this method could liberate glycans without degradation from apo-transferrin containing NeuAc and NeuGc and horseradish peroxidase containing Fuc α1-3 GlcNAc at the reducing end.
29723274	5	26	contain	containing	906:915	arg1	apo-transferrin					890:904	apo-transferrin	890:904	apo-transferrin containing NeuAc and NeuGc and horseradish peroxidase containing Fuc α1-3 GlcNAc at the reducing end	890:1005	Here, we demonstrated N-linked glycan analysis using this method for a monoclonal antibody, and examined whether this method could liberate glycans without degradation from apo-transferrin containing NeuAc and NeuGc and horseradish peroxidase containing Fuc α1-3 GlcNAc at the reducing end.
29723274	2	27	theme	glycoproteins	359:371	arg1	carboxamide					344:354	the asparagine glycosyl carboxamide	320:354	the asparagine glycosyl carboxamide of glycoproteins	320:371	For N-linked glycan analysis, we investigated alkaline hydrolysis of the asparagine glycosyl carboxamide of glycoproteins as a deglycosylation reaction.
29723274	2	28	theme	carboxamide	344:354	arg1	reaction					394:401	a deglycosylation reaction	376:401	a deglycosylation reaction	376:401	For N-linked glycan analysis, we investigated alkaline hydrolysis of the asparagine glycosyl carboxamide of glycoproteins as a deglycosylation reaction.
29723274	2	28	theme	carboxamide	344:354	arg1	hydrolysis					306:315	alkaline hydrolysis	297:315	alkaline hydrolysis of the asparagine glycosyl carboxamide of glycoproteins	297:371	For N-linked glycan analysis, we investigated alkaline hydrolysis of the asparagine glycosyl carboxamide of glycoproteins as a deglycosylation reaction.
29723274	1	29	theme	biopharmaceuticals	231:248	arg1	glycosylation					214:226	glycosylation	214:226	glycosylation of biopharmaceuticals	214:248	Glycan analysis may result in exploitation of glycan biomarkers and evaluation of heterogeneity of glycosylation of biopharmaceuticals.
29723274	6	30	theme	glycan	1033:1038	arg1	recoveries					1040:1049	glycan recoveries	1033:1049	glycan recoveries	1033:1049	Furthermore, we compared glycan recoveries between conventional enzymatic glycan release and this method.
29723274	7	31	theme	reaction	1154:1161	arg1	duration					1163:1170	reaction duration	1154:1170	reaction duration	1154:1170	Increasing the reaction temperature and reaction duration led to degradation, whereas decreasing these parameters resulted in lower release.
29723274	4	32	contain	containing	635:644	arg2	oximes					646:651	oximes	646:651	oximes	646:651	The reaction was completed within 1 h, and the mixture containing oximes was easily tagged with 2-aminobenzamide by reductive amination.
29723274	4	32	contain	containing	635:644	arg1	mixture					627:633	the mixture	623:633	the mixture containing oximes	623:651	The reaction was completed within 1 h, and the mixture containing oximes was easily tagged with 2-aminobenzamide by reductive amination.
29723274	3	33	theme	glycans	504:510	arg1	degradation					480:490	the degradation	476:490	the degradation of released glycans	476:510	By adding hydroxylamine into alkaline de-N-glycosylation, we suppressed the degradation of released glycans and obtained a mixture of oximes, free glycans, and glycosylamines.
29723274	4	34	theme	reductive	696:704	arg1	amination					706:714	reductive amination	696:714	reductive amination	696:714	The reaction was completed within 1 h, and the mixture containing oximes was easily tagged with 2-aminobenzamide by reductive amination.
29723274	7	35	theme	lower	1240:1244	arg1	release					1246:1252	lower release	1240:1252	lower release	1240:1252	Increasing the reaction temperature and reaction duration led to degradation, whereas decreasing these parameters resulted in lower release.
29723274	5	36	theme	Fuc	971:973	arg1	GlcNAc					980:985	Fuc α1-3 GlcNAc	971:985	Fuc α1-3 GlcNAc	971:985	Here, we demonstrated N-linked glycan analysis using this method for a monoclonal antibody, and examined whether this method could liberate glycans without degradation from apo-transferrin containing NeuAc and NeuGc and horseradish peroxidase containing Fuc α1-3 GlcNAc at the reducing end.
29723274	2	37	theme	glycan	264:269	arg1	analysis					271:278	N-linked glycan analysis	255:278	N-linked glycan analysis	255:278	For N-linked glycan analysis, we investigated alkaline hydrolysis of the asparagine glycosyl carboxamide of glycoproteins as a deglycosylation reaction.
29723274	0	38	link	N-linked	97:104	arg1	glycans					106:112	immunoglobulin N-linked glycans	82:112	immunoglobulin N-linked glycans	82:112	Rapid chemical de-N-glycosylation and derivatization for liquid chromatography of immunoglobulin N-linked glycans.
29723274	3	39	theme	glycans	551:557	arg1	mixture					527:533	a mixture	525:533	a mixture of oximes, free glycans, and glycosylamines	525:577	By adding hydroxylamine into alkaline de-N-glycosylation, we suppressed the degradation of released glycans and obtained a mixture of oximes, free glycans, and glycosylamines.
29723274	0	40	theme	liquid	57:62	arg1	chromatography					64:77	liquid chromatography	57:77	liquid chromatography of immunoglobulin N-linked glycans	57:112	Rapid chemical de-N-glycosylation and derivatization for liquid chromatography of immunoglobulin N-linked glycans.
29723274	5	41	theme	N-linked	739:746	arg1	analysis					755:762	N-linked glycan analysis	739:762	N-linked glycan analysis using this method for a monoclonal antibody	739:806	Here, we demonstrated N-linked glycan analysis using this method for a monoclonal antibody, and examined whether this method could liberate glycans without degradation from apo-transferrin containing NeuAc and NeuGc and horseradish peroxidase containing Fuc α1-3 GlcNAc at the reducing end.
29723274	2	42	theme	N-linked	255:262	arg1	analysis					271:278	N-linked glycan analysis	255:278	N-linked glycan analysis	255:278	For N-linked glycan analysis, we investigated alkaline hydrolysis of the asparagine glycosyl carboxamide of glycoproteins as a deglycosylation reaction.
29723274	5	43	theme	reducing	994:1001	arg1	end					1003:1005	the reducing end	990:1005	the reducing end	990:1005	Here, we demonstrated N-linked glycan analysis using this method for a monoclonal antibody, and examined whether this method could liberate glycans without degradation from apo-transferrin containing NeuAc and NeuGc and horseradish peroxidase containing Fuc α1-3 GlcNAc at the reducing end.
29723274	5	44	theme	glycan	748:753	arg1	analysis					755:762	N-linked glycan analysis	739:762	N-linked glycan analysis using this method for a monoclonal antibody	739:806	Here, we demonstrated N-linked glycan analysis using this method for a monoclonal antibody, and examined whether this method could liberate glycans without degradation from apo-transferrin containing NeuAc and NeuGc and horseradish peroxidase containing Fuc α1-3 GlcNAc at the reducing end.
29723274	8	45	gly	glycoproteins	1346:1358	arg1	glycoproteins					1346:1358	asialo glycoproteins	1339:1358	asialo glycoproteins from mammals	1339:1371	Considering this balance, we proposed to carry out the reaction at 80°C for 1 h for asialo glycoproteins from mammals and at 50°C for 1 h for sialoglycoproteins.
29723274	8	46	contain	carry	1296:1300	arg2	reaction					1310:1317	the reaction	1306:1317	the reaction at 80°C for 1 h for asialo glycoproteins from mammals and at 50°C for 1 h for sialoglycoproteins	1306:1414	Considering this balance, we proposed to carry out the reaction at 80°C for 1 h for asialo glycoproteins from mammals and at 50°C for 1 h for sialoglycoproteins.
29723274	8	46	contain	carry	1296:1300	arg1	we					1281:1282	we	1281:1282	we	1281:1282	Considering this balance, we proposed to carry out the reaction at 80°C for 1 h for asialo glycoproteins from mammals and at 50°C for 1 h for sialoglycoproteins.
29723274	2	47	theme	deglycosylation	378:392	arg1	reaction					394:401	a deglycosylation reaction	376:401	a deglycosylation reaction	376:401	For N-linked glycan analysis, we investigated alkaline hydrolysis of the asparagine glycosyl carboxamide of glycoproteins as a deglycosylation reaction.
29723274	2	47	theme	deglycosylation	378:392	arg1	hydrolysis					306:315	alkaline hydrolysis	297:315	alkaline hydrolysis of the asparagine glycosyl carboxamide of glycoproteins	297:371	For N-linked glycan analysis, we investigated alkaline hydrolysis of the asparagine glycosyl carboxamide of glycoproteins as a deglycosylation reaction.
29723274	7	48	theme	reaction	1129:1136	arg1	temperature					1138:1148	the reaction temperature	1125:1148	the reaction temperature	1125:1148	Increasing the reaction temperature and reaction duration led to degradation, whereas decreasing these parameters resulted in lower release.
29723274	2	49	theme	alkaline	297:304	arg1	reaction					394:401	a deglycosylation reaction	376:401	a deglycosylation reaction	376:401	For N-linked glycan analysis, we investigated alkaline hydrolysis of the asparagine glycosyl carboxamide of glycoproteins as a deglycosylation reaction.
29723274	2	49	theme	alkaline	297:304	arg1	hydrolysis					306:315	alkaline hydrolysis	297:315	alkaline hydrolysis of the asparagine glycosyl carboxamide of glycoproteins	297:371	For N-linked glycan analysis, we investigated alkaline hydrolysis of the asparagine glycosyl carboxamide of glycoproteins as a deglycosylation reaction.
29723274	8	50	from	mammals	1365:1371	arg1	glycoproteins					1346:1358	asialo glycoproteins	1339:1358	asialo glycoproteins from mammals	1339:1371	Considering this balance, we proposed to carry out the reaction at 80°C for 1 h for asialo glycoproteins from mammals and at 50°C for 1 h for sialoglycoproteins.
29723274	3	51	theme	free	546:549	arg1	glycans					551:557	free glycans	546:557	free glycans	546:557	By adding hydroxylamine into alkaline de-N-glycosylation, we suppressed the degradation of released glycans and obtained a mixture of oximes, free glycans, and glycosylamines.
29723274	2	52	link	N-linked	255:262	arg1	analysis					271:278	N-linked glycan analysis	255:278	N-linked glycan analysis	255:278	For N-linked glycan analysis, we investigated alkaline hydrolysis of the asparagine glycosyl carboxamide of glycoproteins as a deglycosylation reaction.
29723274	2	53	gly	glycoproteins	359:371	arg1	glycoproteins					359:371	glycoproteins	359:371	glycoproteins	359:371	For N-linked glycan analysis, we investigated alkaline hydrolysis of the asparagine glycosyl carboxamide of glycoproteins as a deglycosylation reaction.
29723274	5	54	theme	α1-3	975:978	arg1	GlcNAc					980:985	Fuc α1-3 GlcNAc	971:985	Fuc α1-3 GlcNAc	971:985	Here, we demonstrated N-linked glycan analysis using this method for a monoclonal antibody, and examined whether this method could liberate glycans without degradation from apo-transferrin containing NeuAc and NeuGc and horseradish peroxidase containing Fuc α1-3 GlcNAc at the reducing end.
29723274	1	55	theme	glycan	161:166	arg1	biomarkers					168:177	glycan biomarkers	161:177	glycan biomarkers	161:177	Glycan analysis may result in exploitation of glycan biomarkers and evaluation of heterogeneity of glycosylation of biopharmaceuticals.
29723274	5	56	theme	horseradish	937:947	arg1	peroxidase					949:958	horseradish peroxidase	937:958	horseradish peroxidase	937:958	Here, we demonstrated N-linked glycan analysis using this method for a monoclonal antibody, and examined whether this method could liberate glycans without degradation from apo-transferrin containing NeuAc and NeuGc and horseradish peroxidase containing Fuc α1-3 GlcNAc at the reducing end.
29723274	1	57	theme	biomarkers	168:177	arg1	exploitation					145:156	exploitation	145:156	exploitation of glycan biomarkers	145:177	Glycan analysis may result in exploitation of glycan biomarkers and evaluation of heterogeneity of glycosylation of biopharmaceuticals.
29723274	1	57	theme	biomarkers	168:177	arg1	evaluation					183:192	evaluation	183:192	evaluation of heterogeneity of glycosylation of biopharmaceuticals	183:248	Glycan analysis may result in exploitation of glycan biomarkers and evaluation of heterogeneity of glycosylation of biopharmaceuticals.
29487451	6	0	theme	mice	1574:1577	arg1	liver					1545:1549	liver	1545:1549	liver	1545:1549	In addition, antioxidant assays in vivo demonstrated that RRTPs can significantly increase the superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) activities, and total antioxidant capacity (TAOC) to some extent, as well as decrease the level of malondialdehyde (MDA) in both serum and liver of d-Gal aging-induced mice.
29487451	6	0	theme	mice	1574:1577	arg1	serum					1535:1539	serum	1535:1539	serum	1535:1539	In addition, antioxidant assays in vivo demonstrated that RRTPs can significantly increase the superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) activities, and total antioxidant capacity (TAOC) to some extent, as well as decrease the level of malondialdehyde (MDA) in both serum and liver of d-Gal aging-induced mice.
29487451	1	1	from	Tratt	314:318	arg1	polysaccharides					277:291	the polysaccharides	273:291	the polysaccharides from Rosa roxburghii Tratt fruit (RRTPs) using ultrasonic-assisted extraction, and the characterization and antioxidant activities of the RRTPs were discussed	273:450	In this study, the response surface methodology was utilized to determine optimum conditions for extracting the polysaccharides from Rosa roxburghii Tratt fruit (RRTPs) using ultrasonic-assisted extraction, and the characterization and antioxidant activities of the RRTPs were discussed.
29487451	2	2	theme	extraction	688:697	arg1	32.8 min					704:711	extraction time 32.8 min	688:711	extraction time 32.8 min	688:711	RRTPs yield was 6.59 ± 1.34%, which was well consistent with the predicted value of 6.716%, under the following optimum conditions: ratio of water to raw material 40.18 mL/g, extraction temperature 78.8 °C, ultrasonic power 148 W, and extraction time 32.8 min.
29487451	2	2	theme	extraction	688:697	arg1	conditions					573:582	the following optimum conditions	551:582	the following optimum conditions: ratio of water to raw material 40.18 mL/g, extraction temperature 78.8 °C, ultrasonic power 148 W, and extraction time 32.8 min	551:711	RRTPs yield was 6.59 ± 1.34%, which was well consistent with the predicted value of 6.716%, under the following optimum conditions: ratio of water to raw material 40.18 mL/g, extraction temperature 78.8 °C, ultrasonic power 148 W, and extraction time 32.8 min.
29487451	0	3	dep	rose	129:132	arg1	tratt					151:155	tratt	151:155	tratt	151:155	Ultrasound-assisted extraction, characterization, and antioxidant activity in vitro and in vivo of polysaccharides from Chestnut rose (Rosa roxburghii tratt) fruit.
29487451	0	3	dep	rose	129:132	arg1	Rosa					135:138	Rosa	135:138	Rosa	135:138	Ultrasound-assisted extraction, characterization, and antioxidant activity in vitro and in vivo of polysaccharides from Chestnut rose (Rosa roxburghii tratt) fruit.
29487451	0	4	from	activity	66:73	arg1	Chestnut					120:127	Chestnut	120:127	Chestnut	120:127	Ultrasound-assisted extraction, characterization, and antioxidant activity in vitro and in vivo of polysaccharides from Chestnut rose (Rosa roxburghii tratt) fruit.
29487451	6	5	from	assays	1257:1262	arg1	vivo					1267:1270	vivo	1267:1270	vivo	1267:1270	In addition, antioxidant assays in vivo demonstrated that RRTPs can significantly increase the superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) activities, and total antioxidant capacity (TAOC) to some extent, as well as decrease the level of malondialdehyde (MDA) in both serum and liver of d-Gal aging-induced mice.
29487451	0	6	from	extraction	20:29	arg1	Chestnut					120:127	Chestnut	120:127	Chestnut	120:127	Ultrasound-assisted extraction, characterization, and antioxidant activity in vitro and in vivo of polysaccharides from Chestnut rose (Rosa roxburghii tratt) fruit.
29487451	8	7	theme	R.	1847:1848	arg1	fruit					1867:1871	value-added R. roxburghii Tratt fruit	1835:1871	value-added R. roxburghii Tratt fruit	1835:1871	In summary, this work provided an effective method for the exploitation and utilization of value-added R. roxburghii Tratt fruit which would be useful to fully utilize this resource.
29487451	2	8	theme	extraction	628:637	arg1	78.8 °C					651:657	extraction temperature 78.8 °C	628:657	extraction temperature 78.8 °C	628:657	RRTPs yield was 6.59 ± 1.34%, which was well consistent with the predicted value of 6.716%, under the following optimum conditions: ratio of water to raw material 40.18 mL/g, extraction temperature 78.8 °C, ultrasonic power 148 W, and extraction time 32.8 min.
29487451	2	8	theme	extraction	628:637	arg1	conditions					573:582	the following optimum conditions	551:582	the following optimum conditions: ratio of water to raw material 40.18 mL/g, extraction temperature 78.8 °C, ultrasonic power 148 W, and extraction time 32.8 min	551:711	RRTPs yield was 6.59 ± 1.34%, which was well consistent with the predicted value of 6.716%, under the following optimum conditions: ratio of water to raw material 40.18 mL/g, extraction temperature 78.8 °C, ultrasonic power 148 W, and extraction time 32.8 min.
29487451	7	9	theme	natural	1646:1652	arg1	antioxidants					1654:1665	natural antioxidants	1646:1665	natural antioxidants	1646:1665	These data suggested that RRTPs could be a potential candidate of natural antioxidants for applications in functional food, pharmaceuticals or cosmetic industries.
29487451	3	10	theme	glucuronic	823:832	arg1	acid					834:837	glucuronic acid	823:837	glucuronic acid (GlcA)	823:844	The monosaccharide composition analysis indicated that RRTPs were composed of mannose (Man), rhamnose (Rha), glucuronic acid (GlcA), galacturonic acid (GalA), glucose (Glc), galactose (Gal), arabinose (Ara) and xylose (Xyl).
29487451	3	10	theme	glucuronic	823:832	arg1	GlcA					840:843	GlcA	840:843	GlcA	840:843	The monosaccharide composition analysis indicated that RRTPs were composed of mannose (Man), rhamnose (Rha), glucuronic acid (GlcA), galacturonic acid (GalA), glucose (Glc), galactose (Gal), arabinose (Ara) and xylose (Xyl).
29487451	1	11	theme	optimum	239:245	arg1	conditions					247:256	optimum conditions	239:256	optimum conditions for extracting the polysaccharides from Rosa roxburghii Tratt fruit (RRTPs) using ultrasonic-assisted extraction, and the characterization and antioxidant activities of the RRTPs were discussed	239:450	In this study, the response surface methodology was utilized to determine optimum conditions for extracting the polysaccharides from Rosa roxburghii Tratt fruit (RRTPs) using ultrasonic-assisted extraction, and the characterization and antioxidant activities of the RRTPs were discussed.
29487451	2	12	theme	predicted	518:526	arg1	value					528:532	the predicted value	514:532	the predicted value of 6.716%	514:542	RRTPs yield was 6.59 ± 1.34%, which was well consistent with the predicted value of 6.716%, under the following optimum conditions: ratio of water to raw material 40.18 mL/g, extraction temperature 78.8 °C, ultrasonic power 148 W, and extraction time 32.8 min.
29487451	4	13	theme	11.92	1067:1071	arg1	weights					1040:1046	molecular weights	1030:1046	molecular weights of 332.56, 183.96, 11.92 and 5.95 kDa, respectively	1030:1098	The molecular weight distribution analysis showed that RRTPs had four main components with molecular weights of 332.56, 183.96, 11.92 and 5.95 kDa, respectively.
29487451	7	14	theme	functional	1687:1696	arg1	food					1698:1701	functional food	1687:1701	functional food	1687:1701	These data suggested that RRTPs could be a potential candidate of natural antioxidants for applications in functional food, pharmaceuticals or cosmetic industries.
29487451	4	15	theme	5.95 kDa	1077:1084	arg1	weights					1040:1046	molecular weights	1030:1046	molecular weights of 332.56, 183.96, 11.92 and 5.95 kDa, respectively	1030:1098	The molecular weight distribution analysis showed that RRTPs had four main components with molecular weights of 332.56, 183.96, 11.92 and 5.95 kDa, respectively.
29487451	1	16	dep	Tratt	314:318	arg1	discussed					442:450	discussed	442:450	were discussed	437:450	In this study, the response surface methodology was utilized to determine optimum conditions for extracting the polysaccharides from Rosa roxburghii Tratt fruit (RRTPs) using ultrasonic-assisted extraction, and the characterization and antioxidant activities of the RRTPs were discussed.
29487451	2	17	theme	optimum	565:571	arg1	78.8 °C					651:657	extraction temperature 78.8 °C	628:657	extraction temperature 78.8 °C	628:657	RRTPs yield was 6.59 ± 1.34%, which was well consistent with the predicted value of 6.716%, under the following optimum conditions: ratio of water to raw material 40.18 mL/g, extraction temperature 78.8 °C, ultrasonic power 148 W, and extraction time 32.8 min.
29487451	2	17	theme	optimum	565:571	arg1	ratio					585:589	ratio	585:589	ratio of water to raw material 40.18 mL/g	585:625	RRTPs yield was 6.59 ± 1.34%, which was well consistent with the predicted value of 6.716%, under the following optimum conditions: ratio of water to raw material 40.18 mL/g, extraction temperature 78.8 °C, ultrasonic power 148 W, and extraction time 32.8 min.
29487451	2	17	theme	optimum	565:571	arg1	32.8 min					704:711	extraction time 32.8 min	688:711	extraction time 32.8 min	688:711	RRTPs yield was 6.59 ± 1.34%, which was well consistent with the predicted value of 6.716%, under the following optimum conditions: ratio of water to raw material 40.18 mL/g, extraction temperature 78.8 °C, ultrasonic power 148 W, and extraction time 32.8 min.
29487451	2	17	theme	optimum	565:571	arg1	conditions					573:582	the following optimum conditions	551:582	the following optimum conditions: ratio of water to raw material 40.18 mL/g, extraction temperature 78.8 °C, ultrasonic power 148 W, and extraction time 32.8 min	551:711	RRTPs yield was 6.59 ± 1.34%, which was well consistent with the predicted value of 6.716%, under the following optimum conditions: ratio of water to raw material 40.18 mL/g, extraction temperature 78.8 °C, ultrasonic power 148 W, and extraction time 32.8 min.
29487451	2	17	theme	optimum	565:571	arg1	148 W					677:681	ultrasonic power 148 W	660:681	ultrasonic power 148 W	660:681	RRTPs yield was 6.59 ± 1.34%, which was well consistent with the predicted value of 6.716%, under the following optimum conditions: ratio of water to raw material 40.18 mL/g, extraction temperature 78.8 °C, ultrasonic power 148 W, and extraction time 32.8 min.
29487451	3	18	theme	galacturonic	847:858	arg1	GalA					866:869	GalA	866:869	GalA	866:869	The monosaccharide composition analysis indicated that RRTPs were composed of mannose (Man), rhamnose (Rha), glucuronic acid (GlcA), galacturonic acid (GalA), glucose (Glc), galactose (Gal), arabinose (Ara) and xylose (Xyl).
29487451	3	18	theme	galacturonic	847:858	arg1	acid					860:863	galacturonic acid	847:863	galacturonic acid (GalA)	847:870	The monosaccharide composition analysis indicated that RRTPs were composed of mannose (Man), rhamnose (Rha), glucuronic acid (GlcA), galacturonic acid (GalA), glucose (Glc), galactose (Gal), arabinose (Ara) and xylose (Xyl).
29487451	8	19	theme	effective	1778:1786	arg1	method					1788:1793	an effective method	1775:1793	an effective method for the exploitation and utilization of value-added R. roxburghii Tratt fruit which would be useful to fully utilize this resource	1775:1924	In summary, this work provided an effective method for the exploitation and utilization of value-added R. roxburghii Tratt fruit which would be useful to fully utilize this resource.
29487451	8	19	theme	effective	1778:1786	arg1	useful					1888:1893	useful	1888:1893	useful	1888:1893	In summary, this work provided an effective method for the exploitation and utilization of value-added R. roxburghii Tratt fruit which would be useful to fully utilize this resource.
29487451	8	20	theme	value-added	1835:1845	arg1	fruit					1867:1871	value-added R. roxburghii Tratt fruit	1835:1871	value-added R. roxburghii Tratt fruit	1835:1871	In summary, this work provided an effective method for the exploitation and utilization of value-added R. roxburghii Tratt fruit which would be useful to fully utilize this resource.
29487451	2	21	theme	raw	603:605	arg1	40.18 mL/g					616:625	raw material 40.18 mL/g	603:625	raw material 40.18 mL/g	603:625	RRTPs yield was 6.59 ± 1.34%, which was well consistent with the predicted value of 6.716%, under the following optimum conditions: ratio of water to raw material 40.18 mL/g, extraction temperature 78.8 °C, ultrasonic power 148 W, and extraction time 32.8 min.
29487451	2	22	theme	material	607:614	arg1	40.18 mL/g					616:625	raw material 40.18 mL/g	603:625	raw material 40.18 mL/g	603:625	RRTPs yield was 6.59 ± 1.34%, which was well consistent with the predicted value of 6.716%, under the following optimum conditions: ratio of water to raw material 40.18 mL/g, extraction temperature 78.8 °C, ultrasonic power 148 W, and extraction time 32.8 min.
29487451	5	23	theme	DPPH	1217:1220	arg1	radicals					1222:1229	hydroxyl, superoxide and DPPH radicals	1192:1229	radicals	1222:1229	In vitro antioxidant studies revealed RRTPs exhibited significant antioxidant potential on hydroxyl, superoxide and DPPH radicals.
29487451	0	24	theme	polysaccharides	99:113	arg1	characterization					32:47	characterization	32:47	characterization	32:47	Ultrasound-assisted extraction, characterization, and antioxidant activity in vitro and in vivo of polysaccharides from Chestnut rose (Rosa roxburghii tratt) fruit.
29487451	0	24	theme	polysaccharides	99:113	arg1	extraction					20:29	Ultrasound-assisted extraction	0:29	Ultrasound-assisted extraction	0:29	Ultrasound-assisted extraction, characterization, and antioxidant activity in vitro and in vivo of polysaccharides from Chestnut rose (Rosa roxburghii tratt) fruit.
29487451	0	24	theme	polysaccharides	99:113	arg1	activity					66:73	antioxidant activity	54:73	antioxidant activity	54:73	Ultrasound-assisted extraction, characterization, and antioxidant activity in vitro and in vivo of polysaccharides from Chestnut rose (Rosa roxburghii tratt) fruit.
29487451	7	25	theme	potential	1623:1631	arg1	candidate					1633:1641	a potential candidate	1621:1641	a potential candidate of natural antioxidants for applications in functional food, pharmaceuticals or cosmetic industries	1621:1741	These data suggested that RRTPs could be a potential candidate of natural antioxidants for applications in functional food, pharmaceuticals or cosmetic industries.
29487451	7	25	theme	potential	1623:1631	arg1	RRTPs					1606:1610	RRTPs	1606:1610	RRTPs	1606:1610	These data suggested that RRTPs could be a potential candidate of natural antioxidants for applications in functional food, pharmaceuticals or cosmetic industries.
29487451	2	26	theme	ultrasonic	660:669	arg1	148 W					677:681	ultrasonic power 148 W	660:681	ultrasonic power 148 W	660:681	RRTPs yield was 6.59 ± 1.34%, which was well consistent with the predicted value of 6.716%, under the following optimum conditions: ratio of water to raw material 40.18 mL/g, extraction temperature 78.8 °C, ultrasonic power 148 W, and extraction time 32.8 min.
29487451	2	26	theme	ultrasonic	660:669	arg1	conditions					573:582	the following optimum conditions	551:582	the following optimum conditions: ratio of water to raw material 40.18 mL/g, extraction temperature 78.8 °C, ultrasonic power 148 W, and extraction time 32.8 min	551:711	RRTPs yield was 6.59 ± 1.34%, which was well consistent with the predicted value of 6.716%, under the following optimum conditions: ratio of water to raw material 40.18 mL/g, extraction temperature 78.8 °C, ultrasonic power 148 W, and extraction time 32.8 min.
29487451	6	27	theme	superoxide	1327:1336	arg1	SOD					1349:1351	SOD	1349:1351	SOD	1349:1351	In addition, antioxidant assays in vivo demonstrated that RRTPs can significantly increase the superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) activities, and total antioxidant capacity (TAOC) to some extent, as well as decrease the level of malondialdehyde (MDA) in both serum and liver of d-Gal aging-induced mice.
29487451	6	27	theme	superoxide	1327:1336	arg1	dismutase					1338:1346	the superoxide dismutase	1323:1346	the superoxide dismutase (SOD)	1323:1352	In addition, antioxidant assays in vivo demonstrated that RRTPs can significantly increase the superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) activities, and total antioxidant capacity (TAOC) to some extent, as well as decrease the level of malondialdehyde (MDA) in both serum and liver of d-Gal aging-induced mice.
29487451	2	28	theme	RRTPs	453:457	arg1	yield					459:463	RRTPs yield	453:463	RRTPs yield	453:463	RRTPs yield was 6.59 ± 1.34%, which was well consistent with the predicted value of 6.716%, under the following optimum conditions: ratio of water to raw material 40.18 mL/g, extraction temperature 78.8 °C, ultrasonic power 148 W, and extraction time 32.8 min.
29487451	2	29	theme	water	594:598	arg1	78.8 °C					651:657	extraction temperature 78.8 °C	628:657	extraction temperature 78.8 °C	628:657	RRTPs yield was 6.59 ± 1.34%, which was well consistent with the predicted value of 6.716%, under the following optimum conditions: ratio of water to raw material 40.18 mL/g, extraction temperature 78.8 °C, ultrasonic power 148 W, and extraction time 32.8 min.
29487451	2	29	theme	water	594:598	arg1	ratio					585:589	ratio	585:589	ratio of water to raw material 40.18 mL/g	585:625	RRTPs yield was 6.59 ± 1.34%, which was well consistent with the predicted value of 6.716%, under the following optimum conditions: ratio of water to raw material 40.18 mL/g, extraction temperature 78.8 °C, ultrasonic power 148 W, and extraction time 32.8 min.
29487451	2	29	theme	water	594:598	arg1	32.8 min					704:711	extraction time 32.8 min	688:711	extraction time 32.8 min	688:711	RRTPs yield was 6.59 ± 1.34%, which was well consistent with the predicted value of 6.716%, under the following optimum conditions: ratio of water to raw material 40.18 mL/g, extraction temperature 78.8 °C, ultrasonic power 148 W, and extraction time 32.8 min.
29487451	2	29	theme	water	594:598	arg1	148 W					677:681	ultrasonic power 148 W	660:681	ultrasonic power 148 W	660:681	RRTPs yield was 6.59 ± 1.34%, which was well consistent with the predicted value of 6.716%, under the following optimum conditions: ratio of water to raw material 40.18 mL/g, extraction temperature 78.8 °C, ultrasonic power 148 W, and extraction time 32.8 min.
29487451	2	29	theme	water	594:598	arg1	conditions					573:582	the following optimum conditions	551:582	the following optimum conditions: ratio of water to raw material 40.18 mL/g, extraction temperature 78.8 °C, ultrasonic power 148 W, and extraction time 32.8 min	551:711	RRTPs yield was 6.59 ± 1.34%, which was well consistent with the predicted value of 6.716%, under the following optimum conditions: ratio of water to raw material 40.18 mL/g, extraction temperature 78.8 °C, ultrasonic power 148 W, and extraction time 32.8 min.
29487451	2	30	with	consistent	498:507	arg1	value					528:532	the predicted value	514:532	the predicted value of 6.716%	514:542	RRTPs yield was 6.59 ± 1.34%, which was well consistent with the predicted value of 6.716%, under the following optimum conditions: ratio of water to raw material 40.18 mL/g, extraction temperature 78.8 °C, ultrasonic power 148 W, and extraction time 32.8 min.
29487451	4	31	theme	main	1009:1012	arg1	components					1014:1023	four main components	1004:1023	four main components	1004:1023	The molecular weight distribution analysis showed that RRTPs had four main components with molecular weights of 332.56, 183.96, 11.92 and 5.95 kDa, respectively.
29487451	0	32	theme	Ultrasound-assisted	0:18	arg1	extraction					20:29	Ultrasound-assisted extraction	0:29	Ultrasound-assisted extraction	0:29	Ultrasound-assisted extraction, characterization, and antioxidant activity in vitro and in vivo of polysaccharides from Chestnut rose (Rosa roxburghii tratt) fruit.
29487451	3	33	theme	composition	733:743	arg1	analysis					745:752	The monosaccharide composition analysis	714:752	The monosaccharide composition analysis	714:752	The monosaccharide composition analysis indicated that RRTPs were composed of mannose (Man), rhamnose (Rha), glucuronic acid (GlcA), galacturonic acid (GalA), glucose (Glc), galactose (Gal), arabinose (Ara) and xylose (Xyl).
29487451	8	34	dep	exploitation	1803:1814	arg1	the					1799:1801	the	1799:1801	the	1799:1801	In summary, this work provided an effective method for the exploitation and utilization of value-added R. roxburghii Tratt fruit which would be useful to fully utilize this resource.
29487451	6	35	theme	antioxidant	1428:1438	arg1	TAOC					1450:1453	TAOC	1450:1453	TAOC	1450:1453	In addition, antioxidant assays in vivo demonstrated that RRTPs can significantly increase the superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) activities, and total antioxidant capacity (TAOC) to some extent, as well as decrease the level of malondialdehyde (MDA) in both serum and liver of d-Gal aging-induced mice.
29487451	6	35	theme	antioxidant	1428:1438	arg1	capacity					1440:1447	total antioxidant capacity	1422:1447	total antioxidant capacity (TAOC) to some extent	1422:1469	In addition, antioxidant assays in vivo demonstrated that RRTPs can significantly increase the superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) activities, and total antioxidant capacity (TAOC) to some extent, as well as decrease the level of malondialdehyde (MDA) in both serum and liver of d-Gal aging-induced mice.
29487451	8	36	theme	Tratt	1861:1865	arg1	fruit					1867:1871	value-added R. roxburghii Tratt fruit	1835:1871	value-added R. roxburghii Tratt fruit	1835:1871	In summary, this work provided an effective method for the exploitation and utilization of value-added R. roxburghii Tratt fruit which would be useful to fully utilize this resource.
29487451	2	37	theme	%	542:542	arg1	value					528:532	the predicted value	514:532	the predicted value of 6.716%	514:542	RRTPs yield was 6.59 ± 1.34%, which was well consistent with the predicted value of 6.716%, under the following optimum conditions: ratio of water to raw material 40.18 mL/g, extraction temperature 78.8 °C, ultrasonic power 148 W, and extraction time 32.8 min.
29487451	2	38	theme	temperature	639:649	arg1	78.8 °C					651:657	extraction temperature 78.8 °C	628:657	extraction temperature 78.8 °C	628:657	RRTPs yield was 6.59 ± 1.34%, which was well consistent with the predicted value of 6.716%, under the following optimum conditions: ratio of water to raw material 40.18 mL/g, extraction temperature 78.8 °C, ultrasonic power 148 W, and extraction time 32.8 min.
29487451	2	38	theme	temperature	639:649	arg1	conditions					573:582	the following optimum conditions	551:582	the following optimum conditions: ratio of water to raw material 40.18 mL/g, extraction temperature 78.8 °C, ultrasonic power 148 W, and extraction time 32.8 min	551:711	RRTPs yield was 6.59 ± 1.34%, which was well consistent with the predicted value of 6.716%, under the following optimum conditions: ratio of water to raw material 40.18 mL/g, extraction temperature 78.8 °C, ultrasonic power 148 W, and extraction time 32.8 min.
29487451	5	39	theme	hydroxyl	1192:1199	arg1	radicals					1222:1229	hydroxyl, superoxide and DPPH radicals	1192:1229	radicals	1222:1229	In vitro antioxidant studies revealed RRTPs exhibited significant antioxidant potential on hydroxyl, superoxide and DPPH radicals.
29487451	7	40	theme	cosmetic	1723:1730	arg1	industries					1732:1741	cosmetic industries	1723:1741	cosmetic industries	1723:1741	These data suggested that RRTPs could be a potential candidate of natural antioxidants for applications in functional food, pharmaceuticals or cosmetic industries.
29487451	6	41	theme	malondialdehyde	1505:1519	arg1	level					1496:1500	the level	1492:1500	the level of malondialdehyde (MDA) in both serum and liver of d-Gal aging-induced mice	1492:1577	In addition, antioxidant assays in vivo demonstrated that RRTPs can significantly increase the superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) activities, and total antioxidant capacity (TAOC) to some extent, as well as decrease the level of malondialdehyde (MDA) in both serum and liver of d-Gal aging-induced mice.
29487451	5	42	theme	antioxidant	1167:1177	arg1	potential					1179:1187	significant antioxidant potential	1155:1187	significant antioxidant potential	1155:1187	In vitro antioxidant studies revealed RRTPs exhibited significant antioxidant potential on hydroxyl, superoxide and DPPH radicals.
29487451	5	43	dep	In	1101:1102	arg1	vitro					1104:1108	vitro	1104:1108	vitro	1104:1108	In vitro antioxidant studies revealed RRTPs exhibited significant antioxidant potential on hydroxyl, superoxide and DPPH radicals.
29487451	6	44	theme	peroxidase	1367:1376	arg1	activities					1406:1415	glutathione peroxidase (GSH-Px) and catalase (CAT) activities	1355:1415	glutathione peroxidase (GSH-Px) and catalase (CAT) activities	1355:1415	In addition, antioxidant assays in vivo demonstrated that RRTPs can significantly increase the superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) activities, and total antioxidant capacity (TAOC) to some extent, as well as decrease the level of malondialdehyde (MDA) in both serum and liver of d-Gal aging-induced mice.
29487451	2	45	dep	conditions	573:582	arg1	78.8 °C					651:657	extraction temperature 78.8 °C	628:657	extraction temperature 78.8 °C	628:657	RRTPs yield was 6.59 ± 1.34%, which was well consistent with the predicted value of 6.716%, under the following optimum conditions: ratio of water to raw material 40.18 mL/g, extraction temperature 78.8 °C, ultrasonic power 148 W, and extraction time 32.8 min.
29487451	2	45	dep	conditions	573:582	arg1	ratio					585:589	ratio	585:589	ratio of water to raw material 40.18 mL/g	585:625	RRTPs yield was 6.59 ± 1.34%, which was well consistent with the predicted value of 6.716%, under the following optimum conditions: ratio of water to raw material 40.18 mL/g, extraction temperature 78.8 °C, ultrasonic power 148 W, and extraction time 32.8 min.
29487451	2	45	dep	conditions	573:582	arg1	32.8 min					704:711	extraction time 32.8 min	688:711	extraction time 32.8 min	688:711	RRTPs yield was 6.59 ± 1.34%, which was well consistent with the predicted value of 6.716%, under the following optimum conditions: ratio of water to raw material 40.18 mL/g, extraction temperature 78.8 °C, ultrasonic power 148 W, and extraction time 32.8 min.
29487451	2	45	dep	conditions	573:582	arg1	148 W					677:681	ultrasonic power 148 W	660:681	ultrasonic power 148 W	660:681	RRTPs yield was 6.59 ± 1.34%, which was well consistent with the predicted value of 6.716%, under the following optimum conditions: ratio of water to raw material 40.18 mL/g, extraction temperature 78.8 °C, ultrasonic power 148 W, and extraction time 32.8 min.
29487451	2	45	dep	conditions	573:582	arg1	conditions					573:582	the following optimum conditions	551:582	the following optimum conditions: ratio of water to raw material 40.18 mL/g, extraction temperature 78.8 °C, ultrasonic power 148 W, and extraction time 32.8 min	551:711	RRTPs yield was 6.59 ± 1.34%, which was well consistent with the predicted value of 6.716%, under the following optimum conditions: ratio of water to raw material 40.18 mL/g, extraction temperature 78.8 °C, ultrasonic power 148 W, and extraction time 32.8 min.
29487451	3	46	theme	monosaccharide	718:731	arg1	analysis					745:752	The monosaccharide composition analysis	714:752	The monosaccharide composition analysis	714:752	The monosaccharide composition analysis indicated that RRTPs were composed of mannose (Man), rhamnose (Rha), glucuronic acid (GlcA), galacturonic acid (GalA), glucose (Glc), galactose (Gal), arabinose (Ara) and xylose (Xyl).
29487451	7	47	from	applications	1671:1682	arg1	pharmaceuticals					1704:1718	pharmaceuticals	1704:1718	pharmaceuticals	1704:1718	These data suggested that RRTPs could be a potential candidate of natural antioxidants for applications in functional food, pharmaceuticals or cosmetic industries.
29487451	7	47	from	applications	1671:1682	arg1	industries					1732:1741	cosmetic industries	1723:1741	cosmetic industries	1723:1741	These data suggested that RRTPs could be a potential candidate of natural antioxidants for applications in functional food, pharmaceuticals or cosmetic industries.
29487451	7	47	from	applications	1671:1682	arg1	food					1698:1701	functional food	1687:1701	functional food	1687:1701	These data suggested that RRTPs could be a potential candidate of natural antioxidants for applications in functional food, pharmaceuticals or cosmetic industries.
29487451	5	48	theme	superoxide	1202:1211	arg1	radicals					1222:1229	hydroxyl, superoxide and DPPH radicals	1192:1229	radicals	1222:1229	In vitro antioxidant studies revealed RRTPs exhibited significant antioxidant potential on hydroxyl, superoxide and DPPH radicals.
29487451	1	49	theme	response	184:191	arg1	methodology					201:211	the response surface methodology	180:211	the response surface methodology	180:211	In this study, the response surface methodology was utilized to determine optimum conditions for extracting the polysaccharides from Rosa roxburghii Tratt fruit (RRTPs) using ultrasonic-assisted extraction, and the characterization and antioxidant activities of the RRTPs were discussed.
29487451	6	50	from	level	1496:1500	arg1	liver					1545:1549	liver	1545:1549	liver	1545:1549	In addition, antioxidant assays in vivo demonstrated that RRTPs can significantly increase the superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) activities, and total antioxidant capacity (TAOC) to some extent, as well as decrease the level of malondialdehyde (MDA) in both serum and liver of d-Gal aging-induced mice.
29487451	6	50	from	level	1496:1500	arg1	serum					1535:1539	serum	1535:1539	serum	1535:1539	In addition, antioxidant assays in vivo demonstrated that RRTPs can significantly increase the superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) activities, and total antioxidant capacity (TAOC) to some extent, as well as decrease the level of malondialdehyde (MDA) in both serum and liver of d-Gal aging-induced mice.
29487451	6	51	theme	aging-induced	1560:1572	arg1	mice					1574:1577	d-Gal aging-induced mice	1554:1577	d-Gal aging-induced mice	1554:1577	In addition, antioxidant assays in vivo demonstrated that RRTPs can significantly increase the superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) activities, and total antioxidant capacity (TAOC) to some extent, as well as decrease the level of malondialdehyde (MDA) in both serum and liver of d-Gal aging-induced mice.
29487451	6	52	theme	total	1422:1426	arg1	TAOC					1450:1453	TAOC	1450:1453	TAOC	1450:1453	In addition, antioxidant assays in vivo demonstrated that RRTPs can significantly increase the superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) activities, and total antioxidant capacity (TAOC) to some extent, as well as decrease the level of malondialdehyde (MDA) in both serum and liver of d-Gal aging-induced mice.
29487451	6	52	theme	total	1422:1426	arg1	capacity					1440:1447	total antioxidant capacity	1422:1447	total antioxidant capacity (TAOC) to some extent	1422:1469	In addition, antioxidant assays in vivo demonstrated that RRTPs can significantly increase the superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) activities, and total antioxidant capacity (TAOC) to some extent, as well as decrease the level of malondialdehyde (MDA) in both serum and liver of d-Gal aging-induced mice.
29487451	1	53	theme	surface	193:199	arg1	methodology					201:211	the response surface methodology	180:211	the response surface methodology	180:211	In this study, the response surface methodology was utilized to determine optimum conditions for extracting the polysaccharides from Rosa roxburghii Tratt fruit (RRTPs) using ultrasonic-assisted extraction, and the characterization and antioxidant activities of the RRTPs were discussed.
29487451	6	54	theme	antioxidant	1245:1255	arg1	assays					1257:1262	antioxidant assays	1245:1262	antioxidant assays in vivo	1245:1270	In addition, antioxidant assays in vivo demonstrated that RRTPs can significantly increase the superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) activities, and total antioxidant capacity (TAOC) to some extent, as well as decrease the level of malondialdehyde (MDA) in both serum and liver of d-Gal aging-induced mice.
29487451	2	55	theme	following	555:563	arg1	78.8 °C					651:657	extraction temperature 78.8 °C	628:657	extraction temperature 78.8 °C	628:657	RRTPs yield was 6.59 ± 1.34%, which was well consistent with the predicted value of 6.716%, under the following optimum conditions: ratio of water to raw material 40.18 mL/g, extraction temperature 78.8 °C, ultrasonic power 148 W, and extraction time 32.8 min.
29487451	2	55	theme	following	555:563	arg1	ratio					585:589	ratio	585:589	ratio of water to raw material 40.18 mL/g	585:625	RRTPs yield was 6.59 ± 1.34%, which was well consistent with the predicted value of 6.716%, under the following optimum conditions: ratio of water to raw material 40.18 mL/g, extraction temperature 78.8 °C, ultrasonic power 148 W, and extraction time 32.8 min.
29487451	2	55	theme	following	555:563	arg1	32.8 min					704:711	extraction time 32.8 min	688:711	extraction time 32.8 min	688:711	RRTPs yield was 6.59 ± 1.34%, which was well consistent with the predicted value of 6.716%, under the following optimum conditions: ratio of water to raw material 40.18 mL/g, extraction temperature 78.8 °C, ultrasonic power 148 W, and extraction time 32.8 min.
29487451	2	55	theme	following	555:563	arg1	conditions					573:582	the following optimum conditions	551:582	the following optimum conditions: ratio of water to raw material 40.18 mL/g, extraction temperature 78.8 °C, ultrasonic power 148 W, and extraction time 32.8 min	551:711	RRTPs yield was 6.59 ± 1.34%, which was well consistent with the predicted value of 6.716%, under the following optimum conditions: ratio of water to raw material 40.18 mL/g, extraction temperature 78.8 °C, ultrasonic power 148 W, and extraction time 32.8 min.
29487451	2	55	theme	following	555:563	arg1	148 W					677:681	ultrasonic power 148 W	660:681	ultrasonic power 148 W	660:681	RRTPs yield was 6.59 ± 1.34%, which was well consistent with the predicted value of 6.716%, under the following optimum conditions: ratio of water to raw material 40.18 mL/g, extraction temperature 78.8 °C, ultrasonic power 148 W, and extraction time 32.8 min.
29487451	1	56	theme	antioxidant	401:411	arg1	activities					413:422	antioxidant activities	401:422	antioxidant activities	401:422	In this study, the response surface methodology was utilized to determine optimum conditions for extracting the polysaccharides from Rosa roxburghii Tratt fruit (RRTPs) using ultrasonic-assisted extraction, and the characterization and antioxidant activities of the RRTPs were discussed.
29487451	4	57	theme	332.56	1051:1056	arg1	weights					1040:1046	molecular weights	1030:1046	molecular weights of 332.56, 183.96, 11.92 and 5.95 kDa, respectively	1030:1098	The molecular weight distribution analysis showed that RRTPs had four main components with molecular weights of 332.56, 183.96, 11.92 and 5.95 kDa, respectively.
29487451	0	58	theme	antioxidant	54:64	arg1	activity					66:73	antioxidant activity	54:73	antioxidant activity	54:73	Ultrasound-assisted extraction, characterization, and antioxidant activity in vitro and in vivo of polysaccharides from Chestnut rose (Rosa roxburghii tratt) fruit.
29487451	6	59	theme	d-Gal	1554:1558	arg1	mice					1574:1577	d-Gal aging-induced mice	1554:1577	d-Gal aging-induced mice	1554:1577	In addition, antioxidant assays in vivo demonstrated that RRTPs can significantly increase the superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) activities, and total antioxidant capacity (TAOC) to some extent, as well as decrease the level of malondialdehyde (MDA) in both serum and liver of d-Gal aging-induced mice.
29487451	6	60	theme	glutathione	1355:1365	arg1	peroxidase					1367:1376	glutathione peroxidase	1355:1376	glutathione peroxidase (GSH-Px)	1355:1385	In addition, antioxidant assays in vivo demonstrated that RRTPs can significantly increase the superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) activities, and total antioxidant capacity (TAOC) to some extent, as well as decrease the level of malondialdehyde (MDA) in both serum and liver of d-Gal aging-induced mice.
29487451	6	60	theme	glutathione	1355:1365	arg1	GSH-Px					1379:1384	GSH-Px	1379:1384	GSH-Px	1379:1384	In addition, antioxidant assays in vivo demonstrated that RRTPs can significantly increase the superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) activities, and total antioxidant capacity (TAOC) to some extent, as well as decrease the level of malondialdehyde (MDA) in both serum and liver of d-Gal aging-induced mice.
29487451	7	61	theme	antioxidants	1654:1665	arg1	candidate					1633:1641	a potential candidate	1621:1641	a potential candidate of natural antioxidants for applications in functional food, pharmaceuticals or cosmetic industries	1621:1741	These data suggested that RRTPs could be a potential candidate of natural antioxidants for applications in functional food, pharmaceuticals or cosmetic industries.
29487451	7	61	theme	antioxidants	1654:1665	arg1	RRTPs					1606:1610	RRTPs	1606:1610	RRTPs	1606:1610	These data suggested that RRTPs could be a potential candidate of natural antioxidants for applications in functional food, pharmaceuticals or cosmetic industries.
29487451	4	62	theme	molecular	943:951	arg1	weight					953:958	The molecular weight	939:958	The molecular weight distribution analysis	939:980	The molecular weight distribution analysis showed that RRTPs had four main components with molecular weights of 332.56, 183.96, 11.92 and 5.95 kDa, respectively.
29487451	1	63	theme	fruit	320:324	arg1	RRTPs					327:331	fruit (RRTPs)	320:332	fruit (RRTPs) using ultrasonic-assisted extraction, and the characterization and antioxidant activities of the RRTPs	320:435	In this study, the response surface methodology was utilized to determine optimum conditions for extracting the polysaccharides from Rosa roxburghii Tratt fruit (RRTPs) using ultrasonic-assisted extraction, and the characterization and antioxidant activities of the RRTPs were discussed.
29487451	4	64	contain	had	1000:1002	arg2	components					1014:1023	four main components	1004:1023	four main components	1004:1023	The molecular weight distribution analysis showed that RRTPs had four main components with molecular weights of 332.56, 183.96, 11.92 and 5.95 kDa, respectively.
29487451	4	64	contain	had	1000:1002	arg1	RRTPs					994:998	RRTPs	994:998	RRTPs	994:998	The molecular weight distribution analysis showed that RRTPs had four main components with molecular weights of 332.56, 183.96, 11.92 and 5.95 kDa, respectively.
29487451	1	65	used	utilized	217:224	arg2	methodology					201:211	the response surface methodology	180:211	the response surface methodology	180:211	In this study, the response surface methodology was utilized to determine optimum conditions for extracting the polysaccharides from Rosa roxburghii Tratt fruit (RRTPs) using ultrasonic-assisted extraction, and the characterization and antioxidant activities of the RRTPs were discussed.
29487451	2	66	theme	power	671:675	arg1	148 W					677:681	ultrasonic power 148 W	660:681	ultrasonic power 148 W	660:681	RRTPs yield was 6.59 ± 1.34%, which was well consistent with the predicted value of 6.716%, under the following optimum conditions: ratio of water to raw material 40.18 mL/g, extraction temperature 78.8 °C, ultrasonic power 148 W, and extraction time 32.8 min.
29487451	2	66	theme	power	671:675	arg1	conditions					573:582	the following optimum conditions	551:582	the following optimum conditions: ratio of water to raw material 40.18 mL/g, extraction temperature 78.8 °C, ultrasonic power 148 W, and extraction time 32.8 min	551:711	RRTPs yield was 6.59 ± 1.34%, which was well consistent with the predicted value of 6.716%, under the following optimum conditions: ratio of water to raw material 40.18 mL/g, extraction temperature 78.8 °C, ultrasonic power 148 W, and extraction time 32.8 min.
29487451	8	67	theme	fruit	1867:1871	arg1	utilization					1820:1830	utilization	1820:1830	utilization	1820:1830	In summary, this work provided an effective method for the exploitation and utilization of value-added R. roxburghii Tratt fruit which would be useful to fully utilize this resource.
29487451	8	67	theme	fruit	1867:1871	arg1	exploitation					1803:1814	exploitation	1803:1814	exploitation	1803:1814	In summary, this work provided an effective method for the exploitation and utilization of value-added R. roxburghii Tratt fruit which would be useful to fully utilize this resource.
29487451	5	68	theme	significant	1155:1165	arg1	potential					1179:1187	significant antioxidant potential	1155:1187	significant antioxidant potential	1155:1187	In vitro antioxidant studies revealed RRTPs exhibited significant antioxidant potential on hydroxyl, superoxide and DPPH radicals.
29487451	4	69	theme	183.96	1059:1064	arg1	weights					1040:1046	molecular weights	1030:1046	molecular weights of 332.56, 183.96, 11.92 and 5.95 kDa, respectively	1030:1098	The molecular weight distribution analysis showed that RRTPs had four main components with molecular weights of 332.56, 183.96, 11.92 and 5.95 kDa, respectively.
29487451	4	70	theme	molecular	1030:1038	arg1	weights					1040:1046	molecular weights	1030:1046	molecular weights of 332.56, 183.96, 11.92 and 5.95 kDa, respectively	1030:1098	The molecular weight distribution analysis showed that RRTPs had four main components with molecular weights of 332.56, 183.96, 11.92 and 5.95 kDa, respectively.
29487451	6	71	theme	catalase	1391:1398	arg1	activities					1406:1415	glutathione peroxidase (GSH-Px) and catalase (CAT) activities	1355:1415	glutathione peroxidase (GSH-Px) and catalase (CAT) activities	1355:1415	In addition, antioxidant assays in vivo demonstrated that RRTPs can significantly increase the superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) activities, and total antioxidant capacity (TAOC) to some extent, as well as decrease the level of malondialdehyde (MDA) in both serum and liver of d-Gal aging-induced mice.
29487451	4	72	theme	distribution	960:971	arg1	analysis					973:980	The molecular weight distribution analysis	939:980	The molecular weight distribution analysis	939:980	The molecular weight distribution analysis showed that RRTPs had four main components with molecular weights of 332.56, 183.96, 11.92 and 5.95 kDa, respectively.
29487451	1	73	theme	RRTPs	431:435	arg1	extraction					360:369	ultrasonic-assisted extraction	340:369	ultrasonic-assisted extraction	340:369	In this study, the response surface methodology was utilized to determine optimum conditions for extracting the polysaccharides from Rosa roxburghii Tratt fruit (RRTPs) using ultrasonic-assisted extraction, and the characterization and antioxidant activities of the RRTPs were discussed.
29487451	1	73	theme	RRTPs	431:435	arg1	characterization					380:395	characterization	380:395	characterization	380:395	In this study, the response surface methodology was utilized to determine optimum conditions for extracting the polysaccharides from Rosa roxburghii Tratt fruit (RRTPs) using ultrasonic-assisted extraction, and the characterization and antioxidant activities of the RRTPs were discussed.
29487451	1	73	theme	RRTPs	431:435	arg1	activities					413:422	antioxidant activities	401:422	antioxidant activities	401:422	In this study, the response surface methodology was utilized to determine optimum conditions for extracting the polysaccharides from Rosa roxburghii Tratt fruit (RRTPs) using ultrasonic-assisted extraction, and the characterization and antioxidant activities of the RRTPs were discussed.
29487451	5	74	theme	antioxidant	1110:1120	arg1	studies					1122:1128	In vitro antioxidant studies	1101:1128	In vitro antioxidant studies	1101:1128	In vitro antioxidant studies revealed RRTPs exhibited significant antioxidant potential on hydroxyl, superoxide and DPPH radicals.
29487451	4	75	theme	weight	953:958	arg1	analysis					973:980	The molecular weight distribution analysis	939:980	The molecular weight distribution analysis	939:980	The molecular weight distribution analysis showed that RRTPs had four main components with molecular weights of 332.56, 183.96, 11.92 and 5.95 kDa, respectively.
29487451	5	76	theme	In	1101:1102	arg1	studies					1122:1128	In vitro antioxidant studies	1101:1128	In vitro antioxidant studies	1101:1128	In vitro antioxidant studies revealed RRTPs exhibited significant antioxidant potential on hydroxyl, superoxide and DPPH radicals.
29487451	0	77	from	characterization	32:47	arg1	Chestnut					120:127	Chestnut	120:127	Chestnut	120:127	Ultrasound-assisted extraction, characterization, and antioxidant activity in vitro and in vivo of polysaccharides from Chestnut rose (Rosa roxburghii tratt) fruit.
29487451	0	78	from	Chestnut	120:127	arg1	characterization					32:47	characterization	32:47	characterization	32:47	Ultrasound-assisted extraction, characterization, and antioxidant activity in vitro and in vivo of polysaccharides from Chestnut rose (Rosa roxburghii tratt) fruit.
29487451	0	78	from	Chestnut	120:127	arg1	polysaccharides					99:113	polysaccharides	99:113	polysaccharides from Chestnut	99:127	Ultrasound-assisted extraction, characterization, and antioxidant activity in vitro and in vivo of polysaccharides from Chestnut rose (Rosa roxburghii tratt) fruit.
29487451	0	78	from	Chestnut	120:127	arg1	extraction					20:29	Ultrasound-assisted extraction	0:29	Ultrasound-assisted extraction	0:29	Ultrasound-assisted extraction, characterization, and antioxidant activity in vitro and in vivo of polysaccharides from Chestnut rose (Rosa roxburghii tratt) fruit.
29487451	0	78	from	Chestnut	120:127	arg1	activity					66:73	antioxidant activity	54:73	antioxidant activity	54:73	Ultrasound-assisted extraction, characterization, and antioxidant activity in vitro and in vivo of polysaccharides from Chestnut rose (Rosa roxburghii tratt) fruit.
29487451	2	79	theme	time	699:702	arg1	32.8 min					704:711	extraction time 32.8 min	688:711	extraction time 32.8 min	688:711	RRTPs yield was 6.59 ± 1.34%, which was well consistent with the predicted value of 6.716%, under the following optimum conditions: ratio of water to raw material 40.18 mL/g, extraction temperature 78.8 °C, ultrasonic power 148 W, and extraction time 32.8 min.
29487451	2	79	theme	time	699:702	arg1	conditions					573:582	the following optimum conditions	551:582	the following optimum conditions: ratio of water to raw material 40.18 mL/g, extraction temperature 78.8 °C, ultrasonic power 148 W, and extraction time 32.8 min	551:711	RRTPs yield was 6.59 ± 1.34%, which was well consistent with the predicted value of 6.716%, under the following optimum conditions: ratio of water to raw material 40.18 mL/g, extraction temperature 78.8 °C, ultrasonic power 148 W, and extraction time 32.8 min.
29487451	8	80	theme	roxburghii	1850:1859	arg1	fruit					1867:1871	value-added R. roxburghii Tratt fruit	1835:1871	value-added R. roxburghii Tratt fruit	1835:1871	In summary, this work provided an effective method for the exploitation and utilization of value-added R. roxburghii Tratt fruit which would be useful to fully utilize this resource.
29487451	1	81	theme	ultrasonic-assisted	340:358	arg1	extraction					360:369	ultrasonic-assisted extraction	340:369	ultrasonic-assisted extraction	340:369	In this study, the response surface methodology was utilized to determine optimum conditions for extracting the polysaccharides from Rosa roxburghii Tratt fruit (RRTPs) using ultrasonic-assisted extraction, and the characterization and antioxidant activities of the RRTPs were discussed.
29487451	1	82	dep	characterization	380:395	arg1	the					376:378	the	376:378	the	376:378	In this study, the response surface methodology was utilized to determine optimum conditions for extracting the polysaccharides from Rosa roxburghii Tratt fruit (RRTPs) using ultrasonic-assisted extraction, and the characterization and antioxidant activities of the RRTPs were discussed.
31782817	3	0	theme	real-time	665:673	arg1	PCR					688:690	real-time quantitative PCR	665:690	real-time quantitative PCR (RT-qPCR)	665:700	Cytokine and Toll-like receptor (TLR) expression were assessed using real-time quantitative PCR (RT-qPCR).
31782817	3	0	theme	real-time	665:673	arg1	RT-qPCR					693:699	RT-qPCR	693:699	RT-qPCR	693:699	Cytokine and Toll-like receptor (TLR) expression were assessed using real-time quantitative PCR (RT-qPCR).
31782817	14	1	theme	RAW264.7	2441:2448	arg1	cells					2450:2454	mouse macrophage RAW264.7 cells	2424:2454	mouse macrophage RAW264.7 cells challenged with lipopolysaccharide	2424:2489	This study investigated the effects of pre-treatments with different components such as surface layer protein, genomic DNA and unmethylated cytosine-phosphate-guanine-containing oligodeoxynucleotides, alone or in combination on immunomodulation, and evaluated the signalling mechanism in mouse macrophage RAW264.7 cells challenged with lipopolysaccharide.
31782817	11	2	theme	nonviable	1865:1873	arg1	components					1889:1898	nonviable probiotic LGG components	1865:1898	nonviable probiotic LGG components	1865:1898	These results indicate that nonviable probiotic LGG components exert an anti-inflammation effect on epithelial cells.
31782817	15	3	theme	extracellular	2640:2652	arg1	kinases					2672:2678	extracellular regulated protein kinases	2640:2678	extracellular regulated protein kinases	2640:2678	Pre-incubation with components alone or in combination generally inhibited the activation of Toll-like receptor, mitogen-activated protein kinases, extracellular regulated protein kinases and nuclear factor-kappa B signalling pathways in lipopolysaccharide-stimulated cells, which generally leads to attenuated inflammatory cytokine interleukin-6 and tumour necrosis factor alpha production.
31782817	6	4	theme	LPS-induced	1164:1174	arg1	IL-6					1191:1194	IL-6	1191:1194	IL-6	1191:1194	Pre-treatment with LGG single components (SLP, gDNA or CpG) or their combinations (SLP + gDNA or SLP + CpG) significantly decreased the LPS-induced interleukin-6 (IL-6) mRNA level (P < 0·05).
31782817	6	4	theme	LPS-induced	1164:1174	arg1	interleukin-6					1176:1188	the LPS-induced interleukin-6	1160:1188	the LPS-induced interleukin-6 (IL-6) mRNA level (P < 0·05)	1160:1217	Pre-treatment with LGG single components (SLP, gDNA or CpG) or their combinations (SLP + gDNA or SLP + CpG) significantly decreased the LPS-induced interleukin-6 (IL-6) mRNA level (P < 0·05).
31782817	14	5	theme	mouse	2424:2428	arg1	cells					2450:2454	mouse macrophage RAW264.7 cells	2424:2454	mouse macrophage RAW264.7 cells challenged with lipopolysaccharide	2424:2489	This study investigated the effects of pre-treatments with different components such as surface layer protein, genomic DNA and unmethylated cytosine-phosphate-guanine-containing oligodeoxynucleotides, alone or in combination on immunomodulation, and evaluated the signalling mechanism in mouse macrophage RAW264.7 cells challenged with lipopolysaccharide.
31782817	10	6	theme	pathways	1732:1739	arg1	activation					1687:1696	the activation	1683:1696	the activation of TLR, MAPK and NF-κB signalling pathways in LPS-stimulated cells	1683:1763	In conclusion, pre-incubation with LGG components, singly or in combination, generally inhibited the activation of TLR, MAPK and NF-κB signalling pathways in LPS-stimulated cells, leading to attenuated inflammatory cytokine TNF-α and IL-6 production.
31782817	1	7	theme	layer	239:243	arg1	protein					245:251	surface layer protein	231:251	surface layer protein	231:251	The aim of this study was to determine whether Lactobacillus rhamnosus GG (LGG) components (surface layer protein, SLP; genomic DNA, gDNA; unmethylated cytosine-phosphate-guanine-containing oligodeoxynucleotide, CpG-ODN), alone or in combination, could affect immunomodulation, and evaluate the signalling mechanism in mouse macrophage RAW264.7 cells challenged with lipopolysaccharide (LPS).
31782817	1	7	theme	layer	239:243	arg1	components					219:228	Lactobacillus rhamnosus GG (LGG) components	186:228	Lactobacillus rhamnosus GG (LGG) components (surface layer protein, SLP; genomic DNA, gDNA; unmethylated cytosine-phosphate-guanine-containing oligodeoxynucleotide, CpG-ODN)	186:358	The aim of this study was to determine whether Lactobacillus rhamnosus GG (LGG) components (surface layer protein, SLP; genomic DNA, gDNA; unmethylated cytosine-phosphate-guanine-containing oligodeoxynucleotide, CpG-ODN), alone or in combination, could affect immunomodulation, and evaluate the signalling mechanism in mouse macrophage RAW264.7 cells challenged with lipopolysaccharide (LPS).
31782817	10	8	from	activation	1687:1696	arg1	cells					1759:1763	LPS-stimulated cells	1744:1763	LPS-stimulated cells	1744:1763	In conclusion, pre-incubation with LGG components, singly or in combination, generally inhibited the activation of TLR, MAPK and NF-κB signalling pathways in LPS-stimulated cells, leading to attenuated inflammatory cytokine TNF-α and IL-6 production.
31782817	1	9	theme	signalling	434:443	arg1	mechanism					445:453	the signalling mechanism	430:453	the signalling mechanism	430:453	The aim of this study was to determine whether Lactobacillus rhamnosus GG (LGG) components (surface layer protein, SLP; genomic DNA, gDNA; unmethylated cytosine-phosphate-guanine-containing oligodeoxynucleotide, CpG-ODN), alone or in combination, could affect immunomodulation, and evaluate the signalling mechanism in mouse macrophage RAW264.7 cells challenged with lipopolysaccharide (LPS).
31782817	10	10	theme	NF-κB	1715:1719	arg1	pathways					1732:1739	TLR, MAPK and NF-κB signalling pathways	1701:1739	pathways	1732:1739	In conclusion, pre-incubation with LGG components, singly or in combination, generally inhibited the activation of TLR, MAPK and NF-κB signalling pathways in LPS-stimulated cells, leading to attenuated inflammatory cytokine TNF-α and IL-6 production.
31782817	1	11	theme	study	155:159	arg1	aim					143:145	The aim	139:145	The aim of this study	139:159	The aim of this study was to determine whether Lactobacillus rhamnosus GG (LGG) components (surface layer protein, SLP; genomic DNA, gDNA; unmethylated cytosine-phosphate-guanine-containing oligodeoxynucleotide, CpG-ODN), alone or in combination, could affect immunomodulation, and evaluate the signalling mechanism in mouse macrophage RAW264.7 cells challenged with lipopolysaccharide (LPS).
31782817	4	12	theme	signalling	824:833	arg1	pathways					835:842	Mitogen-activated protein kinase (MAPK), extracellular regulated protein kinase (ERK) and nuclear factor-kappa B (NF-κB) signalling pathways	703:842	Mitogen-activated protein kinase (MAPK), extracellular regulated protein kinase (ERK) and nuclear factor-kappa B (NF-κB) signalling pathways	703:842	Mitogen-activated protein kinase (MAPK), extracellular regulated protein kinase (ERK) and nuclear factor-kappa B (NF-κB) signalling pathways were evaluated using immunoblots and immunofluorescence.
31782817	16	13	theme	probiotic	2922:2930	arg1	components					2936:2945	nonviable probiotic LGG components	2912:2945	nonviable probiotic LGG components	2912:2945	These results indicate that nonviable probiotic LGG components exert an anti-inflammation effect on epithelial cells.
31782817	14	14	from	mechanism	2411:2419	arg1	cells					2450:2454	mouse macrophage RAW264.7 cells	2424:2454	mouse macrophage RAW264.7 cells challenged with lipopolysaccharide	2424:2489	This study investigated the effects of pre-treatments with different components such as surface layer protein, genomic DNA and unmethylated cytosine-phosphate-guanine-containing oligodeoxynucleotides, alone or in combination on immunomodulation, and evaluated the signalling mechanism in mouse macrophage RAW264.7 cells challenged with lipopolysaccharide.
31782817	6	15	dep	components	1058:1067	arg1	CpG					1083:1085	CpG	1083:1085	CpG	1083:1085	Pre-treatment with LGG single components (SLP, gDNA or CpG) or their combinations (SLP + gDNA or SLP + CpG) significantly decreased the LPS-induced interleukin-6 (IL-6) mRNA level (P < 0·05).
31782817	6	15	dep	components	1058:1067	arg1	gDNA					1075:1078	gDNA	1075:1078	gDNA	1075:1078	Pre-treatment with LGG single components (SLP, gDNA or CpG) or their combinations (SLP + gDNA or SLP + CpG) significantly decreased the LPS-induced interleukin-6 (IL-6) mRNA level (P < 0·05).
31782817	6	15	dep	components	1058:1067	arg1	SLP					1070:1072	SLP	1070:1072	SLP	1070:1072	Pre-treatment with LGG single components (SLP, gDNA or CpG) or their combinations (SLP + gDNA or SLP + CpG) significantly decreased the LPS-induced interleukin-6 (IL-6) mRNA level (P < 0·05).
31782817	6	15	dep	components	1058:1067	arg1	components					1058:1067	LGG single components	1047:1067	LGG single components (SLP, gDNA or CpG)	1047:1086	Pre-treatment with LGG single components (SLP, gDNA or CpG) or their combinations (SLP + gDNA or SLP + CpG) significantly decreased the LPS-induced interleukin-6 (IL-6) mRNA level (P < 0·05).
31782817	10	16	theme	TLR	1701:1703	arg1	pathways					1732:1739	TLR, MAPK and NF-κB signalling pathways	1701:1739	pathways	1732:1739	In conclusion, pre-incubation with LGG components, singly or in combination, generally inhibited the activation of TLR, MAPK and NF-κB signalling pathways in LPS-stimulated cells, leading to attenuated inflammatory cytokine TNF-α and IL-6 production.
31782817	15	17	theme	signalling	2707:2716	arg1	pathways					2718:2725	signalling pathways	2707:2725	signalling pathways	2707:2725	Pre-incubation with components alone or in combination generally inhibited the activation of Toll-like receptor, mitogen-activated protein kinases, extracellular regulated protein kinases and nuclear factor-kappa B signalling pathways in lipopolysaccharide-stimulated cells, which generally leads to attenuated inflammatory cytokine interleukin-6 and tumour necrosis factor alpha production.
31782817	9	18	theme	LGG	1465:1467	arg1	components					1476:1485	LGG single components	1465:1485	LGG single components	1465:1485	Pre-treatment with LGG single components or combinations significantly suppressed the LPS-induced phosphorylation levels of ERK (P > 0·05).
31782817	16	19	theme	nonviable	2912:2920	arg1	components					2936:2945	nonviable probiotic LGG components	2912:2945	nonviable probiotic LGG components	2912:2945	These results indicate that nonviable probiotic LGG components exert an anti-inflammation effect on epithelial cells.
31782817	0	20	theme	lipopolysaccharide	109:126	arg1	challenge					128:136	lipopolysaccharide challenge	109:136	lipopolysaccharide challenge	109:136	Lactobacillus rhamnosus GG components, SLP, gDNA and CpG, exert protective effects on mouse macrophages upon lipopolysaccharide challenge.
31782817	10	21	theme	inflammatory	1788:1799	arg1	TNF-α					1810:1814	attenuated inflammatory cytokine TNF-α	1777:1814	attenuated inflammatory cytokine TNF-α	1777:1814	In conclusion, pre-incubation with LGG components, singly or in combination, generally inhibited the activation of TLR, MAPK and NF-κB signalling pathways in LPS-stimulated cells, leading to attenuated inflammatory cytokine TNF-α and IL-6 production.
31782817	8	22	theme	TLR9	1429:1432	arg1	expression					1415:1424	the LPS-induced expression	1399:1424	the LPS-induced expression of TLR9 (P < 0·05)	1399:1443	SLP pre-treatment also significantly decreased the LPS-induced expression of TLR9 (P < 0·05).
31782817	16	23	theme	LGG	2932:2934	arg1	components					2936:2945	nonviable probiotic LGG components	2912:2945	nonviable probiotic LGG components	2912:2945	These results indicate that nonviable probiotic LGG components exert an anti-inflammation effect on epithelial cells.
31782817	5	24	theme	SLP	901:903	arg1	pre-treatment					919:931	SLP or SLP + gDNA pre-treatment	901:931	SLP or SLP + gDNA pre-treatment	901:931	SLP or SLP + gDNA pre-treatment significantly reduced the LPS-induced mRNA expression of tumour necrosis factor alpha (TNF-α).
31782817	4	25	theme	Mitogen-activated	703:719	arg1	MAPK					737:740	MAPK	737:740	MAPK	737:740	Mitogen-activated protein kinase (MAPK), extracellular regulated protein kinase (ERK) and nuclear factor-kappa B (NF-κB) signalling pathways were evaluated using immunoblots and immunofluorescence.
31782817	4	25	theme	Mitogen-activated	703:719	arg1	kinase					729:734	Mitogen-activated protein kinase	703:734	Mitogen-activated protein kinase (MAPK)	703:741	Mitogen-activated protein kinase (MAPK), extracellular regulated protein kinase (ERK) and nuclear factor-kappa B (NF-κB) signalling pathways were evaluated using immunoblots and immunofluorescence.
31782817	3	26	theme	Toll-like	609:617	arg1	TLR					629:631	Toll-like receptor (TLR)	609:632	Toll-like receptor (TLR)	609:632	Cytokine and Toll-like receptor (TLR) expression were assessed using real-time quantitative PCR (RT-qPCR).
31782817	5	27	theme	SLP + gDNA	908:917	arg1	pre-treatment					919:931	SLP or SLP + gDNA pre-treatment	901:931	SLP or SLP + gDNA pre-treatment	901:931	SLP or SLP + gDNA pre-treatment significantly reduced the LPS-induced mRNA expression of tumour necrosis factor alpha (TNF-α).
31782817	12	28	used	used	2036:2039	arg2	IMPACT					1972:1977	IMPACT	1972:1977	IMPACT	1972:1977	SIGNIFICANCE AND IMPACT OF THE STUDY: Lactobacillus rhamnosus GG (LGG) is widely used as probiotics.
31782817	12	28	used	used	2036:2039	arg2	SIGNIFICANCE					1955:1966	SIGNIFICANCE	1955:1966	SIGNIFICANCE	1955:1966	SIGNIFICANCE AND IMPACT OF THE STUDY: Lactobacillus rhamnosus GG (LGG) is widely used as probiotics.
31782817	12	28	used	used	2036:2039	arg2	probiotics					2044:2053	probiotics	2044:2053	probiotics	2044:2053	SIGNIFICANCE AND IMPACT OF THE STUDY: Lactobacillus rhamnosus GG (LGG) is widely used as probiotics.
31782817	15	29	theme	tumour	2843:2848	arg1	alpha					2866:2870	tumour necrosis factor alpha	2843:2870	tumour necrosis factor alpha	2843:2870	Pre-incubation with components alone or in combination generally inhibited the activation of Toll-like receptor, mitogen-activated protein kinases, extracellular regulated protein kinases and nuclear factor-kappa B signalling pathways in lipopolysaccharide-stimulated cells, which generally leads to attenuated inflammatory cytokine interleukin-6 and tumour necrosis factor alpha production.
31782817	3	30	theme	Cytokine	596:603	arg1	expression					634:643	Cytokine and Toll-like receptor (TLR) expression	596:643	Cytokine and Toll-like receptor (TLR) expression	596:643	Cytokine and Toll-like receptor (TLR) expression were assessed using real-time quantitative PCR (RT-qPCR).
31782817	15	31	with	Pre-incubation	2492:2505	arg1	components					2512:2521	components	2512:2521	components	2512:2521	Pre-incubation with components alone or in combination generally inhibited the activation of Toll-like receptor, mitogen-activated protein kinases, extracellular regulated protein kinases and nuclear factor-kappa B signalling pathways in lipopolysaccharide-stimulated cells, which generally leads to attenuated inflammatory cytokine interleukin-6 and tumour necrosis factor alpha production.
31782817	15	32	theme	interleukin-6	2825:2837	arg1	production					2872:2881	attenuated inflammatory cytokine interleukin-6 and tumour necrosis factor alpha production	2792:2881	attenuated inflammatory cytokine interleukin-6 and tumour necrosis factor alpha production	2792:2881	Pre-incubation with components alone or in combination generally inhibited the activation of Toll-like receptor, mitogen-activated protein kinases, extracellular regulated protein kinases and nuclear factor-kappa B signalling pathways in lipopolysaccharide-stimulated cells, which generally leads to attenuated inflammatory cytokine interleukin-6 and tumour necrosis factor alpha production.
31782817	1	33	theme	cytosine-phosphate-guanine-containing	291:327	arg1	oligodeoxynucleotide					329:348	unmethylated cytosine-phosphate-guanine-containing oligodeoxynucleotide	278:348	unmethylated cytosine-phosphate-guanine-containing oligodeoxynucleotide	278:348	The aim of this study was to determine whether Lactobacillus rhamnosus GG (LGG) components (surface layer protein, SLP; genomic DNA, gDNA; unmethylated cytosine-phosphate-guanine-containing oligodeoxynucleotide, CpG-ODN), alone or in combination, could affect immunomodulation, and evaluate the signalling mechanism in mouse macrophage RAW264.7 cells challenged with lipopolysaccharide (LPS).
31782817	15	34	theme	kinases	2631:2637	arg1	activation					2571:2580	the activation	2567:2580	the activation of Toll-like receptor, mitogen-activated protein kinases, extracellular regulated protein kinases and nuclear factor-kappa B signalling pathways in lipopolysaccharide-stimulated cells	2567:2764	Pre-incubation with components alone or in combination generally inhibited the activation of Toll-like receptor, mitogen-activated protein kinases, extracellular regulated protein kinases and nuclear factor-kappa B signalling pathways in lipopolysaccharide-stimulated cells, which generally leads to attenuated inflammatory cytokine interleukin-6 and tumour necrosis factor alpha production.
31782817	10	35	theme	IL-6	1820:1823	arg1	production					1825:1834	IL-6 production	1820:1834	IL-6 production	1820:1834	In conclusion, pre-incubation with LGG components, singly or in combination, generally inhibited the activation of TLR, MAPK and NF-κB signalling pathways in LPS-stimulated cells, leading to attenuated inflammatory cytokine TNF-α and IL-6 production.
31782817	12	36	dep	SIGNIFICANCE	1955:1966	arg1	Lactobacillus					1993:2005	Lactobacillus	1993:2005	Lactobacillus	1993:2005	SIGNIFICANCE AND IMPACT OF THE STUDY: Lactobacillus rhamnosus GG (LGG) is widely used as probiotics.
31782817	12	36	dep	SIGNIFICANCE	1955:1966	arg1	LGG					2021:2023	LGG	2021:2023	LGG	2021:2023	SIGNIFICANCE AND IMPACT OF THE STUDY: Lactobacillus rhamnosus GG (LGG) is widely used as probiotics.
31782817	0	37	theme	protective	64:73	arg1	effects					75:81	protective effects	64:81	protective effects	64:81	Lactobacillus rhamnosus GG components, SLP, gDNA and CpG, exert protective effects on mouse macrophages upon lipopolysaccharide challenge.
31782817	15	38	theme	mitogen-activated	2605:2621	arg1	kinases					2631:2637	mitogen-activated protein kinases	2605:2637	mitogen-activated protein kinases	2605:2637	Pre-incubation with components alone or in combination generally inhibited the activation of Toll-like receptor, mitogen-activated protein kinases, extracellular regulated protein kinases and nuclear factor-kappa B signalling pathways in lipopolysaccharide-stimulated cells, which generally leads to attenuated inflammatory cytokine interleukin-6 and tumour necrosis factor alpha production.
31782817	9	39	theme	LPS-induced	1532:1542	arg1	levels					1560:1565	the LPS-induced phosphorylation levels	1528:1565	the LPS-induced phosphorylation levels of ERK (P > 0·05)	1528:1583	Pre-treatment with LGG single components or combinations significantly suppressed the LPS-induced phosphorylation levels of ERK (P > 0·05).
31782817	4	40	theme	regulated	758:766	arg1	ERK					784:786	ERK	784:786	ERK	784:786	Mitogen-activated protein kinase (MAPK), extracellular regulated protein kinase (ERK) and nuclear factor-kappa B (NF-κB) signalling pathways were evaluated using immunoblots and immunofluorescence.
31782817	4	40	theme	regulated	758:766	arg1	kinase					776:781	extracellular regulated protein kinase	744:781	extracellular regulated protein kinase (ERK)	744:787	Mitogen-activated protein kinase (MAPK), extracellular regulated protein kinase (ERK) and nuclear factor-kappa B (NF-κB) signalling pathways were evaluated using immunoblots and immunofluorescence.
31782817	15	41	dep	inhibited	2557:2565	arg1	leads					2783:2787	leads	2783:2787	leads to attenuated inflammatory cytokine interleukin-6 and tumour necrosis factor alpha production	2783:2881	Pre-incubation with components alone or in combination generally inhibited the activation of Toll-like receptor, mitogen-activated protein kinases, extracellular regulated protein kinases and nuclear factor-kappa B signalling pathways in lipopolysaccharide-stimulated cells, which generally leads to attenuated inflammatory cytokine interleukin-6 and tumour necrosis factor alpha production.
31782817	15	42	theme	receptor	2595:2602	arg1	activation					2571:2580	the activation	2567:2580	the activation of Toll-like receptor, mitogen-activated protein kinases, extracellular regulated protein kinases and nuclear factor-kappa B signalling pathways in lipopolysaccharide-stimulated cells	2567:2764	Pre-incubation with components alone or in combination generally inhibited the activation of Toll-like receptor, mitogen-activated protein kinases, extracellular regulated protein kinases and nuclear factor-kappa B signalling pathways in lipopolysaccharide-stimulated cells, which generally leads to attenuated inflammatory cytokine interleukin-6 and tumour necrosis factor alpha production.
31782817	7	43	theme	LPS-induced	1302:1312	arg1	TLR2					1314:1317	LPS-induced TLR2	1302:1317	LPS-induced TLR2	1302:1317	Pre-treatment with SLP or gDNA, alone or in combination, significantly suppressed LPS-induced TLR2 and TLR4 mRNA levels (P < 0·05).
31782817	7	43	theme	LPS-induced	1302:1312	arg1	P < 0·05					1341:1348	P < 0·05	1341:1348	P < 0·05	1341:1348	Pre-treatment with SLP or gDNA, alone or in combination, significantly suppressed LPS-induced TLR2 and TLR4 mRNA levels (P < 0·05).
31782817	4	44	theme	kinase	729:734	arg1	pathways					835:842	Mitogen-activated protein kinase (MAPK), extracellular regulated protein kinase (ERK) and nuclear factor-kappa B (NF-κB) signalling pathways	703:842	Mitogen-activated protein kinase (MAPK), extracellular regulated protein kinase (ERK) and nuclear factor-kappa B (NF-κB) signalling pathways	703:842	Mitogen-activated protein kinase (MAPK), extracellular regulated protein kinase (ERK) and nuclear factor-kappa B (NF-κB) signalling pathways were evaluated using immunoblots and immunofluorescence.
31782817	14	45	theme	unmethylated	2263:2274	arg1	oligodeoxynucleotides					2314:2334	unmethylated cytosine-phosphate-guanine-containing oligodeoxynucleotides	2263:2334	unmethylated cytosine-phosphate-guanine-containing oligodeoxynucleotides	2263:2334	This study investigated the effects of pre-treatments with different components such as surface layer protein, genomic DNA and unmethylated cytosine-phosphate-guanine-containing oligodeoxynucleotides, alone or in combination on immunomodulation, and evaluated the signalling mechanism in mouse macrophage RAW264.7 cells challenged with lipopolysaccharide.
31782817	14	45	theme	unmethylated	2263:2274	arg1	protein					2238:2244	surface layer protein	2224:2244	surface layer protein	2224:2244	This study investigated the effects of pre-treatments with different components such as surface layer protein, genomic DNA and unmethylated cytosine-phosphate-guanine-containing oligodeoxynucleotides, alone or in combination on immunomodulation, and evaluated the signalling mechanism in mouse macrophage RAW264.7 cells challenged with lipopolysaccharide.
31782817	0	46	theme	Lactobacillus	0:12	arg1	SLP					39:41	SLP	39:41	SLP	39:41	Lactobacillus rhamnosus GG components, SLP, gDNA and CpG, exert protective effects on mouse macrophages upon lipopolysaccharide challenge.
31782817	0	46	theme	Lactobacillus	0:12	arg1	components					27:36	Lactobacillus rhamnosus GG components	0:36	Lactobacillus rhamnosus GG components	0:36	Lactobacillus rhamnosus GG components, SLP, gDNA and CpG, exert protective effects on mouse macrophages upon lipopolysaccharide challenge.
31782817	0	46	theme	Lactobacillus	0:12	arg1	CpG					53:55	CpG	53:55	CpG	53:55	Lactobacillus rhamnosus GG components, SLP, gDNA and CpG, exert protective effects on mouse macrophages upon lipopolysaccharide challenge.
31782817	0	46	theme	Lactobacillus	0:12	arg1	gDNA					44:47	gDNA	44:47	gDNA	44:47	Lactobacillus rhamnosus GG components, SLP, gDNA and CpG, exert protective effects on mouse macrophages upon lipopolysaccharide challenge.
31782817	9	47	theme	ERK	1570:1572	arg1	levels					1560:1565	the LPS-induced phosphorylation levels	1528:1565	the LPS-induced phosphorylation levels of ERK (P > 0·05)	1528:1583	Pre-treatment with LGG single components or combinations significantly suppressed the LPS-induced phosphorylation levels of ERK (P > 0·05).
31782817	16	48	theme	anti-inflammation	2956:2972	arg1	effect					2974:2979	an anti-inflammation effect	2953:2979	an anti-inflammation effect	2953:2979	These results indicate that nonviable probiotic LGG components exert an anti-inflammation effect on epithelial cells.
31782817	11	49	theme	epithelial	1937:1946	arg1	cells					1948:1952	epithelial cells	1937:1952	epithelial cells	1937:1952	These results indicate that nonviable probiotic LGG components exert an anti-inflammation effect on epithelial cells.
31782817	6	50	theme	LGG	1047:1049	arg1	CpG					1083:1085	CpG	1083:1085	CpG	1083:1085	Pre-treatment with LGG single components (SLP, gDNA or CpG) or their combinations (SLP + gDNA or SLP + CpG) significantly decreased the LPS-induced interleukin-6 (IL-6) mRNA level (P < 0·05).
31782817	6	50	theme	LGG	1047:1049	arg1	gDNA					1075:1078	gDNA	1075:1078	gDNA	1075:1078	Pre-treatment with LGG single components (SLP, gDNA or CpG) or their combinations (SLP + gDNA or SLP + CpG) significantly decreased the LPS-induced interleukin-6 (IL-6) mRNA level (P < 0·05).
31782817	6	50	theme	LGG	1047:1049	arg1	SLP					1070:1072	SLP	1070:1072	SLP	1070:1072	Pre-treatment with LGG single components (SLP, gDNA or CpG) or their combinations (SLP + gDNA or SLP + CpG) significantly decreased the LPS-induced interleukin-6 (IL-6) mRNA level (P < 0·05).
31782817	6	50	theme	LGG	1047:1049	arg1	components					1058:1067	LGG single components	1047:1067	LGG single components (SLP, gDNA or CpG)	1047:1086	Pre-treatment with LGG single components (SLP, gDNA or CpG) or their combinations (SLP + gDNA or SLP + CpG) significantly decreased the LPS-induced interleukin-6 (IL-6) mRNA level (P < 0·05).
31782817	8	51	theme	SLP	1352:1354	arg1	pre-treatment					1356:1368	SLP pre-treatment	1352:1368	SLP pre-treatment	1352:1368	SLP pre-treatment also significantly decreased the LPS-induced expression of TLR9 (P < 0·05).
31782817	1	52	theme	genomic	259:265	arg1	DNA					267:269	genomic DNA	259:269	genomic DNA	259:269	The aim of this study was to determine whether Lactobacillus rhamnosus GG (LGG) components (surface layer protein, SLP; genomic DNA, gDNA; unmethylated cytosine-phosphate-guanine-containing oligodeoxynucleotide, CpG-ODN), alone or in combination, could affect immunomodulation, and evaluate the signalling mechanism in mouse macrophage RAW264.7 cells challenged with lipopolysaccharide (LPS).
31782817	4	53	theme	factor-kappa	801:812	arg1	NF-κB					817:821	NF-κB	817:821	NF-κB	817:821	Mitogen-activated protein kinase (MAPK), extracellular regulated protein kinase (ERK) and nuclear factor-kappa B (NF-κB) signalling pathways were evaluated using immunoblots and immunofluorescence.
31782817	4	53	theme	factor-kappa	801:812	arg1	B					814:814	nuclear factor-kappa B	793:814	nuclear factor-kappa B (NF-κB)	793:822	Mitogen-activated protein kinase (MAPK), extracellular regulated protein kinase (ERK) and nuclear factor-kappa B (NF-κB) signalling pathways were evaluated using immunoblots and immunofluorescence.
31782817	1	54	theme	RAW264.7	475:482	arg1	cells					484:488	mouse macrophage RAW264.7 cells	458:488	mouse macrophage RAW264.7 cells challenged with lipopolysaccharide (LPS)	458:529	The aim of this study was to determine whether Lactobacillus rhamnosus GG (LGG) components (surface layer protein, SLP; genomic DNA, gDNA; unmethylated cytosine-phosphate-guanine-containing oligodeoxynucleotide, CpG-ODN), alone or in combination, could affect immunomodulation, and evaluate the signalling mechanism in mouse macrophage RAW264.7 cells challenged with lipopolysaccharide (LPS).
31782817	15	55	theme	factor	2859:2864	arg1	alpha					2866:2870	tumour necrosis factor alpha	2843:2870	tumour necrosis factor alpha	2843:2870	Pre-incubation with components alone or in combination generally inhibited the activation of Toll-like receptor, mitogen-activated protein kinases, extracellular regulated protein kinases and nuclear factor-kappa B signalling pathways in lipopolysaccharide-stimulated cells, which generally leads to attenuated inflammatory cytokine interleukin-6 and tumour necrosis factor alpha production.
31782817	4	56	theme	kinase	776:781	arg1	pathways					835:842	Mitogen-activated protein kinase (MAPK), extracellular regulated protein kinase (ERK) and nuclear factor-kappa B (NF-κB) signalling pathways	703:842	Mitogen-activated protein kinase (MAPK), extracellular regulated protein kinase (ERK) and nuclear factor-kappa B (NF-κB) signalling pathways	703:842	Mitogen-activated protein kinase (MAPK), extracellular regulated protein kinase (ERK) and nuclear factor-kappa B (NF-κB) signalling pathways were evaluated using immunoblots and immunofluorescence.
31782817	15	57	theme	B	2705:2705	arg1	activation					2571:2580	the activation	2567:2580	the activation of Toll-like receptor, mitogen-activated protein kinases, extracellular regulated protein kinases and nuclear factor-kappa B signalling pathways in lipopolysaccharide-stimulated cells	2567:2764	Pre-incubation with components alone or in combination generally inhibited the activation of Toll-like receptor, mitogen-activated protein kinases, extracellular regulated protein kinases and nuclear factor-kappa B signalling pathways in lipopolysaccharide-stimulated cells, which generally leads to attenuated inflammatory cytokine interleukin-6 and tumour necrosis factor alpha production.
31782817	5	58	theme	necrosis	997:1004	arg1	TNF-α					1020:1024	TNF-α	1020:1024	TNF-α	1020:1024	SLP or SLP + gDNA pre-treatment significantly reduced the LPS-induced mRNA expression of tumour necrosis factor alpha (TNF-α).
31782817	5	58	theme	necrosis	997:1004	arg1	alpha					1013:1017	tumour necrosis factor alpha	990:1017	tumour necrosis factor alpha (TNF-α)	990:1025	SLP or SLP + gDNA pre-treatment significantly reduced the LPS-induced mRNA expression of tumour necrosis factor alpha (TNF-α).
31782817	10	59	with	pre-incubation	1601:1614	arg1	components					1625:1634	LGG components	1621:1634	LGG components	1621:1634	In conclusion, pre-incubation with LGG components, singly or in combination, generally inhibited the activation of TLR, MAPK and NF-κB signalling pathways in LPS-stimulated cells, leading to attenuated inflammatory cytokine TNF-α and IL-6 production.
31782817	8	60	theme	LPS-induced	1403:1413	arg1	expression					1415:1424	the LPS-induced expression	1399:1424	the LPS-induced expression of TLR9 (P < 0·05)	1399:1443	SLP pre-treatment also significantly decreased the LPS-induced expression of TLR9 (P < 0·05).
31782817	11	61	theme	probiotic	1875:1883	arg1	components					1889:1898	nonviable probiotic LGG components	1865:1898	nonviable probiotic LGG components	1865:1898	These results indicate that nonviable probiotic LGG components exert an anti-inflammation effect on epithelial cells.
31782817	15	62	theme	nuclear	2684:2690	arg1	B					2705:2705	nuclear factor-kappa B	2684:2705	nuclear factor-kappa B	2684:2705	Pre-incubation with components alone or in combination generally inhibited the activation of Toll-like receptor, mitogen-activated protein kinases, extracellular regulated protein kinases and nuclear factor-kappa B signalling pathways in lipopolysaccharide-stimulated cells, which generally leads to attenuated inflammatory cytokine interleukin-6 and tumour necrosis factor alpha production.
31782817	5	63	theme	alpha	1013:1017	arg1	expression					976:985	the LPS-induced mRNA expression	955:985	the LPS-induced mRNA expression of tumour necrosis factor alpha (TNF-α)	955:1025	SLP or SLP + gDNA pre-treatment significantly reduced the LPS-induced mRNA expression of tumour necrosis factor alpha (TNF-α).
31782817	14	64	theme	surface	2224:2230	arg1	DNA					2255:2257	genomic DNA	2247:2257	genomic DNA	2247:2257	This study investigated the effects of pre-treatments with different components such as surface layer protein, genomic DNA and unmethylated cytosine-phosphate-guanine-containing oligodeoxynucleotides, alone or in combination on immunomodulation, and evaluated the signalling mechanism in mouse macrophage RAW264.7 cells challenged with lipopolysaccharide.
31782817	14	64	theme	surface	2224:2230	arg1	oligodeoxynucleotides					2314:2334	unmethylated cytosine-phosphate-guanine-containing oligodeoxynucleotides	2263:2334	unmethylated cytosine-phosphate-guanine-containing oligodeoxynucleotides	2263:2334	This study investigated the effects of pre-treatments with different components such as surface layer protein, genomic DNA and unmethylated cytosine-phosphate-guanine-containing oligodeoxynucleotides, alone or in combination on immunomodulation, and evaluated the signalling mechanism in mouse macrophage RAW264.7 cells challenged with lipopolysaccharide.
31782817	14	64	theme	surface	2224:2230	arg1	protein					2238:2244	surface layer protein	2224:2244	surface layer protein	2224:2244	This study investigated the effects of pre-treatments with different components such as surface layer protein, genomic DNA and unmethylated cytosine-phosphate-guanine-containing oligodeoxynucleotides, alone or in combination on immunomodulation, and evaluated the signalling mechanism in mouse macrophage RAW264.7 cells challenged with lipopolysaccharide.
31782817	15	65	theme	kinases	2672:2678	arg1	activation					2571:2580	the activation	2567:2580	the activation of Toll-like receptor, mitogen-activated protein kinases, extracellular regulated protein kinases and nuclear factor-kappa B signalling pathways in lipopolysaccharide-stimulated cells	2567:2764	Pre-incubation with components alone or in combination generally inhibited the activation of Toll-like receptor, mitogen-activated protein kinases, extracellular regulated protein kinases and nuclear factor-kappa B signalling pathways in lipopolysaccharide-stimulated cells, which generally leads to attenuated inflammatory cytokine interleukin-6 and tumour necrosis factor alpha production.
31782817	2	66	theme	LPS	579:581	arg1	stimulation					583:593	LPS stimulation	579:593	LPS stimulation	579:593	LGG components were used to treat cells before LPS stimulation.
31782817	3	67	theme	quantitative	675:686	arg1	PCR					688:690	real-time quantitative PCR	665:690	real-time quantitative PCR (RT-qPCR)	665:700	Cytokine and Toll-like receptor (TLR) expression were assessed using real-time quantitative PCR (RT-qPCR).
31782817	3	67	theme	quantitative	675:686	arg1	RT-qPCR					693:699	RT-qPCR	693:699	RT-qPCR	693:699	Cytokine and Toll-like receptor (TLR) expression were assessed using real-time quantitative PCR (RT-qPCR).
31782817	7	68	with	Pre-treatment	1220:1232	arg1	gDNA					1246:1249	gDNA	1246:1249	gDNA	1246:1249	Pre-treatment with SLP or gDNA, alone or in combination, significantly suppressed LPS-induced TLR2 and TLR4 mRNA levels (P < 0·05).
31782817	7	68	with	Pre-treatment	1220:1232	arg1	SLP					1239:1241	SLP	1239:1241	SLP	1239:1241	Pre-treatment with SLP or gDNA, alone or in combination, significantly suppressed LPS-induced TLR2 and TLR4 mRNA levels (P < 0·05).
31782817	14	69	theme	macrophage	2430:2439	arg1	cells					2450:2454	mouse macrophage RAW264.7 cells	2424:2454	mouse macrophage RAW264.7 cells challenged with lipopolysaccharide	2424:2489	This study investigated the effects of pre-treatments with different components such as surface layer protein, genomic DNA and unmethylated cytosine-phosphate-guanine-containing oligodeoxynucleotides, alone or in combination on immunomodulation, and evaluated the signalling mechanism in mouse macrophage RAW264.7 cells challenged with lipopolysaccharide.
31782817	15	70	theme	regulated	2654:2662	arg1	kinases					2672:2678	extracellular regulated protein kinases	2640:2678	extracellular regulated protein kinases	2640:2678	Pre-incubation with components alone or in combination generally inhibited the activation of Toll-like receptor, mitogen-activated protein kinases, extracellular regulated protein kinases and nuclear factor-kappa B signalling pathways in lipopolysaccharide-stimulated cells, which generally leads to attenuated inflammatory cytokine interleukin-6 and tumour necrosis factor alpha production.
31782817	1	71	theme	surface	231:237	arg1	protein					245:251	surface layer protein	231:251	surface layer protein	231:251	The aim of this study was to determine whether Lactobacillus rhamnosus GG (LGG) components (surface layer protein, SLP; genomic DNA, gDNA; unmethylated cytosine-phosphate-guanine-containing oligodeoxynucleotide, CpG-ODN), alone or in combination, could affect immunomodulation, and evaluate the signalling mechanism in mouse macrophage RAW264.7 cells challenged with lipopolysaccharide (LPS).
31782817	1	71	theme	surface	231:237	arg1	components					219:228	Lactobacillus rhamnosus GG (LGG) components	186:228	Lactobacillus rhamnosus GG (LGG) components (surface layer protein, SLP; genomic DNA, gDNA; unmethylated cytosine-phosphate-guanine-containing oligodeoxynucleotide, CpG-ODN)	186:358	The aim of this study was to determine whether Lactobacillus rhamnosus GG (LGG) components (surface layer protein, SLP; genomic DNA, gDNA; unmethylated cytosine-phosphate-guanine-containing oligodeoxynucleotide, CpG-ODN), alone or in combination, could affect immunomodulation, and evaluate the signalling mechanism in mouse macrophage RAW264.7 cells challenged with lipopolysaccharide (LPS).
31782817	14	72	theme	different	2195:2203	arg1	components					2205:2214	different components	2195:2214	different components such as surface layer protein, genomic DNA and unmethylated cytosine-phosphate-guanine-containing oligodeoxynucleotides, alone or in combination	2195:2359	This study investigated the effects of pre-treatments with different components such as surface layer protein, genomic DNA and unmethylated cytosine-phosphate-guanine-containing oligodeoxynucleotides, alone or in combination on immunomodulation, and evaluated the signalling mechanism in mouse macrophage RAW264.7 cells challenged with lipopolysaccharide.
31782817	14	72	theme	different	2195:2203	arg1	protein					2238:2244	surface layer protein	2224:2244	surface layer protein	2224:2244	This study investigated the effects of pre-treatments with different components such as surface layer protein, genomic DNA and unmethylated cytosine-phosphate-guanine-containing oligodeoxynucleotides, alone or in combination on immunomodulation, and evaluated the signalling mechanism in mouse macrophage RAW264.7 cells challenged with lipopolysaccharide.
31782817	10	73	theme	signalling	1721:1730	arg1	pathways					1732:1739	TLR, MAPK and NF-κB signalling pathways	1701:1739	pathways	1732:1739	In conclusion, pre-incubation with LGG components, singly or in combination, generally inhibited the activation of TLR, MAPK and NF-κB signalling pathways in LPS-stimulated cells, leading to attenuated inflammatory cytokine TNF-α and IL-6 production.
31782817	4	74	theme	B	814:814	arg1	pathways					835:842	Mitogen-activated protein kinase (MAPK), extracellular regulated protein kinase (ERK) and nuclear factor-kappa B (NF-κB) signalling pathways	703:842	Mitogen-activated protein kinase (MAPK), extracellular regulated protein kinase (ERK) and nuclear factor-kappa B (NF-κB) signalling pathways	703:842	Mitogen-activated protein kinase (MAPK), extracellular regulated protein kinase (ERK) and nuclear factor-kappa B (NF-κB) signalling pathways were evaluated using immunoblots and immunofluorescence.
31782817	16	75	theme	epithelial	2984:2993	arg1	cells					2995:2999	epithelial cells	2984:2999	epithelial cells	2984:2999	These results indicate that nonviable probiotic LGG components exert an anti-inflammation effect on epithelial cells.
31782817	14	76	theme	pre-treatments	2175:2188	arg1	effects					2164:2170	the effects	2160:2170	the effects of pre-treatments with different components such as surface layer protein, genomic DNA and unmethylated cytosine-phosphate-guanine-containing oligodeoxynucleotides, alone or in combination on immunomodulation	2160:2379	This study investigated the effects of pre-treatments with different components such as surface layer protein, genomic DNA and unmethylated cytosine-phosphate-guanine-containing oligodeoxynucleotides, alone or in combination on immunomodulation, and evaluated the signalling mechanism in mouse macrophage RAW264.7 cells challenged with lipopolysaccharide.
31782817	14	77	theme	signalling	2400:2409	arg1	mechanism					2411:2419	the signalling mechanism	2396:2419	the signalling mechanism in mouse macrophage RAW264.7 cells challenged with lipopolysaccharide	2396:2489	This study investigated the effects of pre-treatments with different components such as surface layer protein, genomic DNA and unmethylated cytosine-phosphate-guanine-containing oligodeoxynucleotides, alone or in combination on immunomodulation, and evaluated the signalling mechanism in mouse macrophage RAW264.7 cells challenged with lipopolysaccharide.
31782817	14	78	from	effects	2164:2170	arg1	immunomodulation					2364:2379	immunomodulation	2364:2379	immunomodulation	2364:2379	This study investigated the effects of pre-treatments with different components such as surface layer protein, genomic DNA and unmethylated cytosine-phosphate-guanine-containing oligodeoxynucleotides, alone or in combination on immunomodulation, and evaluated the signalling mechanism in mouse macrophage RAW264.7 cells challenged with lipopolysaccharide.
31782817	1	79	theme	mouse	458:462	arg1	cells					484:488	mouse macrophage RAW264.7 cells	458:488	mouse macrophage RAW264.7 cells challenged with lipopolysaccharide (LPS)	458:529	The aim of this study was to determine whether Lactobacillus rhamnosus GG (LGG) components (surface layer protein, SLP; genomic DNA, gDNA; unmethylated cytosine-phosphate-guanine-containing oligodeoxynucleotide, CpG-ODN), alone or in combination, could affect immunomodulation, and evaluate the signalling mechanism in mouse macrophage RAW264.7 cells challenged with lipopolysaccharide (LPS).
31782817	15	80	theme	lipopolysaccharide-stimulated	2730:2758	arg1	cells					2760:2764	lipopolysaccharide-stimulated cells	2730:2764	lipopolysaccharide-stimulated cells	2730:2764	Pre-incubation with components alone or in combination generally inhibited the activation of Toll-like receptor, mitogen-activated protein kinases, extracellular regulated protein kinases and nuclear factor-kappa B signalling pathways in lipopolysaccharide-stimulated cells, which generally leads to attenuated inflammatory cytokine interleukin-6 and tumour necrosis factor alpha production.
31782817	7	81	dep	TLR2	1314:1317	arg1	levels					1333:1338	mRNA levels	1328:1338	mRNA levels	1328:1338	Pre-treatment with SLP or gDNA, alone or in combination, significantly suppressed LPS-induced TLR2 and TLR4 mRNA levels (P < 0·05).
31782817	12	82	theme	STUDY	1986:1990	arg1	probiotics					2044:2053	probiotics	2044:2053	probiotics	2044:2053	SIGNIFICANCE AND IMPACT OF THE STUDY: Lactobacillus rhamnosus GG (LGG) is widely used as probiotics.
31782817	12	82	theme	STUDY	1986:1990	arg1	IMPACT					1972:1977	IMPACT	1972:1977	IMPACT	1972:1977	SIGNIFICANCE AND IMPACT OF THE STUDY: Lactobacillus rhamnosus GG (LGG) is widely used as probiotics.
31782817	12	82	theme	STUDY	1986:1990	arg1	SIGNIFICANCE					1955:1966	SIGNIFICANCE	1955:1966	SIGNIFICANCE	1955:1966	SIGNIFICANCE AND IMPACT OF THE STUDY: Lactobacillus rhamnosus GG (LGG) is widely used as probiotics.
31782817	2	83	theme	LGG	532:534	arg1	components					536:545	LGG components	532:545	LGG components	532:545	LGG components were used to treat cells before LPS stimulation.
31782817	10	84	theme	LGG	1621:1623	arg1	components					1625:1634	LGG components	1621:1634	LGG components	1621:1634	In conclusion, pre-incubation with LGG components, singly or in combination, generally inhibited the activation of TLR, MAPK and NF-κB signalling pathways in LPS-stimulated cells, leading to attenuated inflammatory cytokine TNF-α and IL-6 production.
31782817	10	85	theme	cytokine	1801:1808	arg1	TNF-α					1810:1814	attenuated inflammatory cytokine TNF-α	1777:1814	attenuated inflammatory cytokine TNF-α	1777:1814	In conclusion, pre-incubation with LGG components, singly or in combination, generally inhibited the activation of TLR, MAPK and NF-κB signalling pathways in LPS-stimulated cells, leading to attenuated inflammatory cytokine TNF-α and IL-6 production.
31782817	1	86	theme	Lactobacillus	186:198	arg1	protein					245:251	surface layer protein	231:251	surface layer protein	231:251	The aim of this study was to determine whether Lactobacillus rhamnosus GG (LGG) components (surface layer protein, SLP; genomic DNA, gDNA; unmethylated cytosine-phosphate-guanine-containing oligodeoxynucleotide, CpG-ODN), alone or in combination, could affect immunomodulation, and evaluate the signalling mechanism in mouse macrophage RAW264.7 cells challenged with lipopolysaccharide (LPS).
31782817	1	86	theme	Lactobacillus	186:198	arg1	components					219:228	Lactobacillus rhamnosus GG (LGG) components	186:228	Lactobacillus rhamnosus GG (LGG) components (surface layer protein, SLP; genomic DNA, gDNA; unmethylated cytosine-phosphate-guanine-containing oligodeoxynucleotide, CpG-ODN)	186:358	The aim of this study was to determine whether Lactobacillus rhamnosus GG (LGG) components (surface layer protein, SLP; genomic DNA, gDNA; unmethylated cytosine-phosphate-guanine-containing oligodeoxynucleotide, CpG-ODN), alone or in combination, could affect immunomodulation, and evaluate the signalling mechanism in mouse macrophage RAW264.7 cells challenged with lipopolysaccharide (LPS).
31782817	15	87	from	activation	2571:2580	arg1	cells					2760:2764	lipopolysaccharide-stimulated cells	2730:2764	lipopolysaccharide-stimulated cells	2730:2764	Pre-incubation with components alone or in combination generally inhibited the activation of Toll-like receptor, mitogen-activated protein kinases, extracellular regulated protein kinases and nuclear factor-kappa B signalling pathways in lipopolysaccharide-stimulated cells, which generally leads to attenuated inflammatory cytokine interleukin-6 and tumour necrosis factor alpha production.
31782817	10	88	theme	attenuated	1777:1786	arg1	TNF-α					1810:1814	attenuated inflammatory cytokine TNF-α	1777:1814	attenuated inflammatory cytokine TNF-α	1777:1814	In conclusion, pre-incubation with LGG components, singly or in combination, generally inhibited the activation of TLR, MAPK and NF-κB signalling pathways in LPS-stimulated cells, leading to attenuated inflammatory cytokine TNF-α and IL-6 production.
31782817	6	89	theme	mRNA	1197:1200	arg1	level					1202:1206	the LPS-induced interleukin-6 (IL-6) mRNA level	1160:1206	the LPS-induced interleukin-6 (IL-6) mRNA level (P < 0·05)	1160:1217	Pre-treatment with LGG single components (SLP, gDNA or CpG) or their combinations (SLP + gDNA or SLP + CpG) significantly decreased the LPS-induced interleukin-6 (IL-6) mRNA level (P < 0·05).
31782817	6	89	theme	mRNA	1197:1200	arg1	P < 0·05					1209:1216	P < 0·05	1209:1216	P < 0·05	1209:1216	Pre-treatment with LGG single components (SLP, gDNA or CpG) or their combinations (SLP + gDNA or SLP + CpG) significantly decreased the LPS-induced interleukin-6 (IL-6) mRNA level (P < 0·05).
31782817	1	90	dep	Lactobacillus	186:198	arg1	GG					210:211	Lactobacillus rhamnosus GG	186:211	Lactobacillus rhamnosus GG (LGG) components (surface layer protein, SLP; genomic DNA, gDNA; unmethylated cytosine-phosphate-guanine-containing oligodeoxynucleotide, CpG-ODN)	186:358	The aim of this study was to determine whether Lactobacillus rhamnosus GG (LGG) components (surface layer protein, SLP; genomic DNA, gDNA; unmethylated cytosine-phosphate-guanine-containing oligodeoxynucleotide, CpG-ODN), alone or in combination, could affect immunomodulation, and evaluate the signalling mechanism in mouse macrophage RAW264.7 cells challenged with lipopolysaccharide (LPS).
31782817	9	91	theme	single	1469:1474	arg1	components					1476:1485	LGG single components	1465:1485	LGG single components	1465:1485	Pre-treatment with LGG single components or combinations significantly suppressed the LPS-induced phosphorylation levels of ERK (P > 0·05).
31782817	7	92	theme	mRNA	1328:1331	arg1	levels					1333:1338	mRNA levels	1328:1338	mRNA levels	1328:1338	Pre-treatment with SLP or gDNA, alone or in combination, significantly suppressed LPS-induced TLR2 and TLR4 mRNA levels (P < 0·05).
31782817	2	93	used	used	552:555	arg2	components					536:545	LGG components	532:545	LGG components	532:545	LGG components were used to treat cells before LPS stimulation.
31782817	6	94	theme	interleukin-6	1176:1188	arg1	level					1202:1206	the LPS-induced interleukin-6 (IL-6) mRNA level	1160:1206	the LPS-induced interleukin-6 (IL-6) mRNA level (P < 0·05)	1160:1217	Pre-treatment with LGG single components (SLP, gDNA or CpG) or their combinations (SLP + gDNA or SLP + CpG) significantly decreased the LPS-induced interleukin-6 (IL-6) mRNA level (P < 0·05).
31782817	6	94	theme	interleukin-6	1176:1188	arg1	P < 0·05					1209:1216	P < 0·05	1209:1216	P < 0·05	1209:1216	Pre-treatment with LGG single components (SLP, gDNA or CpG) or their combinations (SLP + gDNA or SLP + CpG) significantly decreased the LPS-induced interleukin-6 (IL-6) mRNA level (P < 0·05).
31782817	15	95	theme	inflammatory	2803:2814	arg1	interleukin-6					2825:2837	inflammatory cytokine interleukin-6	2803:2837	inflammatory cytokine interleukin-6	2803:2837	Pre-incubation with components alone or in combination generally inhibited the activation of Toll-like receptor, mitogen-activated protein kinases, extracellular regulated protein kinases and nuclear factor-kappa B signalling pathways in lipopolysaccharide-stimulated cells, which generally leads to attenuated inflammatory cytokine interleukin-6 and tumour necrosis factor alpha production.
31782817	15	96	theme	cytokine	2816:2823	arg1	interleukin-6					2825:2837	inflammatory cytokine interleukin-6	2803:2837	inflammatory cytokine interleukin-6	2803:2837	Pre-incubation with components alone or in combination generally inhibited the activation of Toll-like receptor, mitogen-activated protein kinases, extracellular regulated protein kinases and nuclear factor-kappa B signalling pathways in lipopolysaccharide-stimulated cells, which generally leads to attenuated inflammatory cytokine interleukin-6 and tumour necrosis factor alpha production.
31782817	1	97	theme	unmethylated	278:289	arg1	oligodeoxynucleotide					329:348	unmethylated cytosine-phosphate-guanine-containing oligodeoxynucleotide	278:348	unmethylated cytosine-phosphate-guanine-containing oligodeoxynucleotide	278:348	The aim of this study was to determine whether Lactobacillus rhamnosus GG (LGG) components (surface layer protein, SLP; genomic DNA, gDNA; unmethylated cytosine-phosphate-guanine-containing oligodeoxynucleotide, CpG-ODN), alone or in combination, could affect immunomodulation, and evaluate the signalling mechanism in mouse macrophage RAW264.7 cells challenged with lipopolysaccharide (LPS).
31782817	1	98	dep	protein	245:251	arg1	SLP					254:256	SLP	254:256	SLP	254:256	The aim of this study was to determine whether Lactobacillus rhamnosus GG (LGG) components (surface layer protein, SLP; genomic DNA, gDNA; unmethylated cytosine-phosphate-guanine-containing oligodeoxynucleotide, CpG-ODN), alone or in combination, could affect immunomodulation, and evaluate the signalling mechanism in mouse macrophage RAW264.7 cells challenged with lipopolysaccharide (LPS).
31782817	1	98	dep	protein	245:251	arg1	CpG-ODN					351:357	CpG-ODN	351:357	CpG-ODN	351:357	The aim of this study was to determine whether Lactobacillus rhamnosus GG (LGG) components (surface layer protein, SLP; genomic DNA, gDNA; unmethylated cytosine-phosphate-guanine-containing oligodeoxynucleotide, CpG-ODN), alone or in combination, could affect immunomodulation, and evaluate the signalling mechanism in mouse macrophage RAW264.7 cells challenged with lipopolysaccharide (LPS).
31782817	1	98	dep	protein	245:251	arg1	gDNA					272:275	gDNA	272:275	gDNA	272:275	The aim of this study was to determine whether Lactobacillus rhamnosus GG (LGG) components (surface layer protein, SLP; genomic DNA, gDNA; unmethylated cytosine-phosphate-guanine-containing oligodeoxynucleotide, CpG-ODN), alone or in combination, could affect immunomodulation, and evaluate the signalling mechanism in mouse macrophage RAW264.7 cells challenged with lipopolysaccharide (LPS).
31782817	1	98	dep	protein	245:251	arg1	oligodeoxynucleotide					329:348	unmethylated cytosine-phosphate-guanine-containing oligodeoxynucleotide	278:348	unmethylated cytosine-phosphate-guanine-containing oligodeoxynucleotide	278:348	The aim of this study was to determine whether Lactobacillus rhamnosus GG (LGG) components (surface layer protein, SLP; genomic DNA, gDNA; unmethylated cytosine-phosphate-guanine-containing oligodeoxynucleotide, CpG-ODN), alone or in combination, could affect immunomodulation, and evaluate the signalling mechanism in mouse macrophage RAW264.7 cells challenged with lipopolysaccharide (LPS).
31782817	1	98	dep	protein	245:251	arg1	DNA					267:269	genomic DNA	259:269	genomic DNA	259:269	The aim of this study was to determine whether Lactobacillus rhamnosus GG (LGG) components (surface layer protein, SLP; genomic DNA, gDNA; unmethylated cytosine-phosphate-guanine-containing oligodeoxynucleotide, CpG-ODN), alone or in combination, could affect immunomodulation, and evaluate the signalling mechanism in mouse macrophage RAW264.7 cells challenged with lipopolysaccharide (LPS).
31782817	15	99	dep	receptor	2595:2602	arg1	pathways					2718:2725	signalling pathways	2707:2725	signalling pathways	2707:2725	Pre-incubation with components alone or in combination generally inhibited the activation of Toll-like receptor, mitogen-activated protein kinases, extracellular regulated protein kinases and nuclear factor-kappa B signalling pathways in lipopolysaccharide-stimulated cells, which generally leads to attenuated inflammatory cytokine interleukin-6 and tumour necrosis factor alpha production.
31782817	15	100	theme	attenuated	2792:2801	arg1	production					2872:2881	attenuated inflammatory cytokine interleukin-6 and tumour necrosis factor alpha production	2792:2881	attenuated inflammatory cytokine interleukin-6 and tumour necrosis factor alpha production	2792:2881	Pre-incubation with components alone or in combination generally inhibited the activation of Toll-like receptor, mitogen-activated protein kinases, extracellular regulated protein kinases and nuclear factor-kappa B signalling pathways in lipopolysaccharide-stimulated cells, which generally leads to attenuated inflammatory cytokine interleukin-6 and tumour necrosis factor alpha production.
31782817	13	101	theme	main	2069:2072	arg1	components					2074:2083	its main components	2065:2083	its main components	2065:2083	However, its main components are not well known for affecting immunomodulation.
31782817	5	102	theme	LPS-induced	959:969	arg1	expression					976:985	the LPS-induced mRNA expression	955:985	the LPS-induced mRNA expression of tumour necrosis factor alpha (TNF-α)	955:1025	SLP or SLP + gDNA pre-treatment significantly reduced the LPS-induced mRNA expression of tumour necrosis factor alpha (TNF-α).
31782817	10	103	theme	LPS-stimulated	1744:1757	arg1	cells					1759:1763	LPS-stimulated cells	1744:1763	LPS-stimulated cells	1744:1763	In conclusion, pre-incubation with LGG components, singly or in combination, generally inhibited the activation of TLR, MAPK and NF-κB signalling pathways in LPS-stimulated cells, leading to attenuated inflammatory cytokine TNF-α and IL-6 production.
31782817	5	104	theme	mRNA	971:974	arg1	expression					976:985	the LPS-induced mRNA expression	955:985	the LPS-induced mRNA expression of tumour necrosis factor alpha (TNF-α)	955:1025	SLP or SLP + gDNA pre-treatment significantly reduced the LPS-induced mRNA expression of tumour necrosis factor alpha (TNF-α).
31782817	0	105	theme	mouse	86:90	arg1	macrophages					92:102	mouse macrophages	86:102	mouse macrophages	86:102	Lactobacillus rhamnosus GG components, SLP, gDNA and CpG, exert protective effects on mouse macrophages upon lipopolysaccharide challenge.
31782817	15	106	theme	protein	2623:2629	arg1	kinases					2631:2637	mitogen-activated protein kinases	2605:2637	mitogen-activated protein kinases	2605:2637	Pre-incubation with components alone or in combination generally inhibited the activation of Toll-like receptor, mitogen-activated protein kinases, extracellular regulated protein kinases and nuclear factor-kappa B signalling pathways in lipopolysaccharide-stimulated cells, which generally leads to attenuated inflammatory cytokine interleukin-6 and tumour necrosis factor alpha production.
31782817	9	107	theme	phosphorylation	1544:1558	arg1	levels					1560:1565	the LPS-induced phosphorylation levels	1528:1565	the LPS-induced phosphorylation levels of ERK (P > 0·05)	1528:1583	Pre-treatment with LGG single components or combinations significantly suppressed the LPS-induced phosphorylation levels of ERK (P > 0·05).
31782817	4	108	theme	extracellular	744:756	arg1	ERK					784:786	ERK	784:786	ERK	784:786	Mitogen-activated protein kinase (MAPK), extracellular regulated protein kinase (ERK) and nuclear factor-kappa B (NF-κB) signalling pathways were evaluated using immunoblots and immunofluorescence.
31782817	4	108	theme	extracellular	744:756	arg1	kinase					776:781	extracellular regulated protein kinase	744:781	extracellular regulated protein kinase (ERK)	744:787	Mitogen-activated protein kinase (MAPK), extracellular regulated protein kinase (ERK) and nuclear factor-kappa B (NF-κB) signalling pathways were evaluated using immunoblots and immunofluorescence.
31782817	14	109	theme	cytosine-phosphate-guanine-containing	2276:2312	arg1	oligodeoxynucleotides					2314:2334	unmethylated cytosine-phosphate-guanine-containing oligodeoxynucleotides	2263:2334	unmethylated cytosine-phosphate-guanine-containing oligodeoxynucleotides	2263:2334	This study investigated the effects of pre-treatments with different components such as surface layer protein, genomic DNA and unmethylated cytosine-phosphate-guanine-containing oligodeoxynucleotides, alone or in combination on immunomodulation, and evaluated the signalling mechanism in mouse macrophage RAW264.7 cells challenged with lipopolysaccharide.
31782817	14	109	theme	cytosine-phosphate-guanine-containing	2276:2312	arg1	protein					2238:2244	surface layer protein	2224:2244	surface layer protein	2224:2244	This study investigated the effects of pre-treatments with different components such as surface layer protein, genomic DNA and unmethylated cytosine-phosphate-guanine-containing oligodeoxynucleotides, alone or in combination on immunomodulation, and evaluated the signalling mechanism in mouse macrophage RAW264.7 cells challenged with lipopolysaccharide.
31782817	11	110	theme	anti-inflammation	1909:1925	arg1	effect					1927:1932	an anti-inflammation effect	1906:1932	an anti-inflammation effect	1906:1932	These results indicate that nonviable probiotic LGG components exert an anti-inflammation effect on epithelial cells.
31782817	4	111	theme	protein	721:727	arg1	MAPK					737:740	MAPK	737:740	MAPK	737:740	Mitogen-activated protein kinase (MAPK), extracellular regulated protein kinase (ERK) and nuclear factor-kappa B (NF-κB) signalling pathways were evaluated using immunoblots and immunofluorescence.
31782817	4	111	theme	protein	721:727	arg1	kinase					729:734	Mitogen-activated protein kinase	703:734	Mitogen-activated protein kinase (MAPK)	703:741	Mitogen-activated protein kinase (MAPK), extracellular regulated protein kinase (ERK) and nuclear factor-kappa B (NF-κB) signalling pathways were evaluated using immunoblots and immunofluorescence.
31782817	15	112	theme	Toll-like	2585:2593	arg1	receptor					2595:2602	Toll-like receptor	2585:2602	Toll-like receptor	2585:2602	Pre-incubation with components alone or in combination generally inhibited the activation of Toll-like receptor, mitogen-activated protein kinases, extracellular regulated protein kinases and nuclear factor-kappa B signalling pathways in lipopolysaccharide-stimulated cells, which generally leads to attenuated inflammatory cytokine interleukin-6 and tumour necrosis factor alpha production.
31782817	6	113	theme	single	1051:1056	arg1	CpG					1083:1085	CpG	1083:1085	CpG	1083:1085	Pre-treatment with LGG single components (SLP, gDNA or CpG) or their combinations (SLP + gDNA or SLP + CpG) significantly decreased the LPS-induced interleukin-6 (IL-6) mRNA level (P < 0·05).
31782817	6	113	theme	single	1051:1056	arg1	gDNA					1075:1078	gDNA	1075:1078	gDNA	1075:1078	Pre-treatment with LGG single components (SLP, gDNA or CpG) or their combinations (SLP + gDNA or SLP + CpG) significantly decreased the LPS-induced interleukin-6 (IL-6) mRNA level (P < 0·05).
31782817	6	113	theme	single	1051:1056	arg1	SLP					1070:1072	SLP	1070:1072	SLP	1070:1072	Pre-treatment with LGG single components (SLP, gDNA or CpG) or their combinations (SLP + gDNA or SLP + CpG) significantly decreased the LPS-induced interleukin-6 (IL-6) mRNA level (P < 0·05).
31782817	6	113	theme	single	1051:1056	arg1	components					1058:1067	LGG single components	1047:1067	LGG single components (SLP, gDNA or CpG)	1047:1086	Pre-treatment with LGG single components (SLP, gDNA or CpG) or their combinations (SLP + gDNA or SLP + CpG) significantly decreased the LPS-induced interleukin-6 (IL-6) mRNA level (P < 0·05).
31782817	9	114	with	Pre-treatment	1446:1458	arg1	combinations					1490:1501	combinations	1490:1501	combinations	1490:1501	Pre-treatment with LGG single components or combinations significantly suppressed the LPS-induced phosphorylation levels of ERK (P > 0·05).
31782817	9	114	with	Pre-treatment	1446:1458	arg1	components					1476:1485	LGG single components	1465:1485	LGG single components	1465:1485	Pre-treatment with LGG single components or combinations significantly suppressed the LPS-induced phosphorylation levels of ERK (P > 0·05).
31782817	3	115	theme	TLR	629:631	arg1	expression					634:643	Cytokine and Toll-like receptor (TLR) expression	596:643	Cytokine and Toll-like receptor (TLR) expression	596:643	Cytokine and Toll-like receptor (TLR) expression were assessed using real-time quantitative PCR (RT-qPCR).
31782817	14	116	theme	genomic	2247:2253	arg1	DNA					2255:2257	genomic DNA	2247:2257	genomic DNA	2247:2257	This study investigated the effects of pre-treatments with different components such as surface layer protein, genomic DNA and unmethylated cytosine-phosphate-guanine-containing oligodeoxynucleotides, alone or in combination on immunomodulation, and evaluated the signalling mechanism in mouse macrophage RAW264.7 cells challenged with lipopolysaccharide.
31782817	14	116	theme	genomic	2247:2253	arg1	protein					2238:2244	surface layer protein	2224:2244	surface layer protein	2224:2244	This study investigated the effects of pre-treatments with different components such as surface layer protein, genomic DNA and unmethylated cytosine-phosphate-guanine-containing oligodeoxynucleotides, alone or in combination on immunomodulation, and evaluated the signalling mechanism in mouse macrophage RAW264.7 cells challenged with lipopolysaccharide.
31782817	15	117	theme	alpha	2866:2870	arg1	production					2872:2881	attenuated inflammatory cytokine interleukin-6 and tumour necrosis factor alpha production	2792:2881	attenuated inflammatory cytokine interleukin-6 and tumour necrosis factor alpha production	2792:2881	Pre-incubation with components alone or in combination generally inhibited the activation of Toll-like receptor, mitogen-activated protein kinases, extracellular regulated protein kinases and nuclear factor-kappa B signalling pathways in lipopolysaccharide-stimulated cells, which generally leads to attenuated inflammatory cytokine interleukin-6 and tumour necrosis factor alpha production.
31782817	12	118	dep	Lactobacillus	1993:2005	arg1	GG					2017:2018	Lactobacillus rhamnosus GG	1993:2018	SIGNIFICANCE AND IMPACT OF THE STUDY: Lactobacillus rhamnosus GG (LGG)	1955:2024	SIGNIFICANCE AND IMPACT OF THE STUDY: Lactobacillus rhamnosus GG (LGG) is widely used as probiotics.
31782817	3	119	theme	receptor	619:626	arg1	TLR					629:631	Toll-like receptor (TLR)	609:632	Toll-like receptor (TLR)	609:632	Cytokine and Toll-like receptor (TLR) expression were assessed using real-time quantitative PCR (RT-qPCR).
31782817	1	120	theme	macrophage	464:473	arg1	cells					484:488	mouse macrophage RAW264.7 cells	458:488	mouse macrophage RAW264.7 cells challenged with lipopolysaccharide (LPS)	458:529	The aim of this study was to determine whether Lactobacillus rhamnosus GG (LGG) components (surface layer protein, SLP; genomic DNA, gDNA; unmethylated cytosine-phosphate-guanine-containing oligodeoxynucleotide, CpG-ODN), alone or in combination, could affect immunomodulation, and evaluate the signalling mechanism in mouse macrophage RAW264.7 cells challenged with lipopolysaccharide (LPS).
31782817	15	121	theme	necrosis	2850:2857	arg1	alpha					2866:2870	tumour necrosis factor alpha	2843:2870	tumour necrosis factor alpha	2843:2870	Pre-incubation with components alone or in combination generally inhibited the activation of Toll-like receptor, mitogen-activated protein kinases, extracellular regulated protein kinases and nuclear factor-kappa B signalling pathways in lipopolysaccharide-stimulated cells, which generally leads to attenuated inflammatory cytokine interleukin-6 and tumour necrosis factor alpha production.
31782817	4	122	theme	nuclear	793:799	arg1	NF-κB					817:821	NF-κB	817:821	NF-κB	817:821	Mitogen-activated protein kinase (MAPK), extracellular regulated protein kinase (ERK) and nuclear factor-kappa B (NF-κB) signalling pathways were evaluated using immunoblots and immunofluorescence.
31782817	4	122	theme	nuclear	793:799	arg1	B					814:814	nuclear factor-kappa B	793:814	nuclear factor-kappa B (NF-κB)	793:822	Mitogen-activated protein kinase (MAPK), extracellular regulated protein kinase (ERK) and nuclear factor-kappa B (NF-κB) signalling pathways were evaluated using immunoblots and immunofluorescence.
31782817	15	123	theme	factor-kappa	2692:2703	arg1	B					2705:2705	nuclear factor-kappa B	2684:2705	nuclear factor-kappa B	2684:2705	Pre-incubation with components alone or in combination generally inhibited the activation of Toll-like receptor, mitogen-activated protein kinases, extracellular regulated protein kinases and nuclear factor-kappa B signalling pathways in lipopolysaccharide-stimulated cells, which generally leads to attenuated inflammatory cytokine interleukin-6 and tumour necrosis factor alpha production.
31782817	0	124	dep	Lactobacillus	0:12	arg1	GG					24:25	Lactobacillus rhamnosus GG	0:25	Lactobacillus rhamnosus GG components	0:36	Lactobacillus rhamnosus GG components, SLP, gDNA and CpG, exert protective effects on mouse macrophages upon lipopolysaccharide challenge.
31782817	6	125	with	Pre-treatment	1028:1040	arg1	combinations					1097:1108	their combinations	1091:1108	their combinations (SLP + gDNA or SLP + CpG)	1091:1134	Pre-treatment with LGG single components (SLP, gDNA or CpG) or their combinations (SLP + gDNA or SLP + CpG) significantly decreased the LPS-induced interleukin-6 (IL-6) mRNA level (P < 0·05).
31782817	6	125	with	Pre-treatment	1028:1040	arg1	gDNA					1075:1078	gDNA	1075:1078	gDNA	1075:1078	Pre-treatment with LGG single components (SLP, gDNA or CpG) or their combinations (SLP + gDNA or SLP + CpG) significantly decreased the LPS-induced interleukin-6 (IL-6) mRNA level (P < 0·05).
31782817	6	125	with	Pre-treatment	1028:1040	arg1	CpG					1083:1085	CpG	1083:1085	CpG	1083:1085	Pre-treatment with LGG single components (SLP, gDNA or CpG) or their combinations (SLP + gDNA or SLP + CpG) significantly decreased the LPS-induced interleukin-6 (IL-6) mRNA level (P < 0·05).
31782817	6	125	with	Pre-treatment	1028:1040	arg1	SLP + gDNA					1111:1120	SLP + gDNA	1111:1120	SLP + gDNA	1111:1120	Pre-treatment with LGG single components (SLP, gDNA or CpG) or their combinations (SLP + gDNA or SLP + CpG) significantly decreased the LPS-induced interleukin-6 (IL-6) mRNA level (P < 0·05).
31782817	6	125	with	Pre-treatment	1028:1040	arg1	components					1058:1067	LGG single components	1047:1067	LGG single components (SLP, gDNA or CpG)	1047:1086	Pre-treatment with LGG single components (SLP, gDNA or CpG) or their combinations (SLP + gDNA or SLP + CpG) significantly decreased the LPS-induced interleukin-6 (IL-6) mRNA level (P < 0·05).
31782817	6	125	with	Pre-treatment	1028:1040	arg1	SLP + CpG					1125:1133	SLP + CpG	1125:1133	SLP + CpG	1125:1133	Pre-treatment with LGG single components (SLP, gDNA or CpG) or their combinations (SLP + gDNA or SLP + CpG) significantly decreased the LPS-induced interleukin-6 (IL-6) mRNA level (P < 0·05).
31782817	6	125	with	Pre-treatment	1028:1040	arg1	SLP					1070:1072	SLP	1070:1072	SLP	1070:1072	Pre-treatment with LGG single components (SLP, gDNA or CpG) or their combinations (SLP + gDNA or SLP + CpG) significantly decreased the LPS-induced interleukin-6 (IL-6) mRNA level (P < 0·05).
31782817	5	126	theme	tumour	990:995	arg1	TNF-α					1020:1024	TNF-α	1020:1024	TNF-α	1020:1024	SLP or SLP + gDNA pre-treatment significantly reduced the LPS-induced mRNA expression of tumour necrosis factor alpha (TNF-α).
31782817	5	126	theme	tumour	990:995	arg1	alpha					1013:1017	tumour necrosis factor alpha	990:1017	tumour necrosis factor alpha (TNF-α)	990:1025	SLP or SLP + gDNA pre-treatment significantly reduced the LPS-induced mRNA expression of tumour necrosis factor alpha (TNF-α).
31782817	4	127	theme	protein	768:774	arg1	ERK					784:786	ERK	784:786	ERK	784:786	Mitogen-activated protein kinase (MAPK), extracellular regulated protein kinase (ERK) and nuclear factor-kappa B (NF-κB) signalling pathways were evaluated using immunoblots and immunofluorescence.
31782817	4	127	theme	protein	768:774	arg1	kinase					776:781	extracellular regulated protein kinase	744:781	extracellular regulated protein kinase (ERK)	744:787	Mitogen-activated protein kinase (MAPK), extracellular regulated protein kinase (ERK) and nuclear factor-kappa B (NF-κB) signalling pathways were evaluated using immunoblots and immunofluorescence.
31782817	10	128	theme	MAPK	1706:1709	arg1	pathways					1732:1739	TLR, MAPK and NF-κB signalling pathways	1701:1739	pathways	1732:1739	In conclusion, pre-incubation with LGG components, singly or in combination, generally inhibited the activation of TLR, MAPK and NF-κB signalling pathways in LPS-stimulated cells, leading to attenuated inflammatory cytokine TNF-α and IL-6 production.
31782817	14	129	with	pre-treatments	2175:2188	arg1	components					2205:2214	different components	2195:2214	different components such as surface layer protein, genomic DNA and unmethylated cytosine-phosphate-guanine-containing oligodeoxynucleotides, alone or in combination	2195:2359	This study investigated the effects of pre-treatments with different components such as surface layer protein, genomic DNA and unmethylated cytosine-phosphate-guanine-containing oligodeoxynucleotides, alone or in combination on immunomodulation, and evaluated the signalling mechanism in mouse macrophage RAW264.7 cells challenged with lipopolysaccharide.
31782817	14	129	with	pre-treatments	2175:2188	arg1	protein					2238:2244	surface layer protein	2224:2244	surface layer protein	2224:2244	This study investigated the effects of pre-treatments with different components such as surface layer protein, genomic DNA and unmethylated cytosine-phosphate-guanine-containing oligodeoxynucleotides, alone or in combination on immunomodulation, and evaluated the signalling mechanism in mouse macrophage RAW264.7 cells challenged with lipopolysaccharide.
31782817	5	130	theme	factor	1006:1011	arg1	TNF-α					1020:1024	TNF-α	1020:1024	TNF-α	1020:1024	SLP or SLP + gDNA pre-treatment significantly reduced the LPS-induced mRNA expression of tumour necrosis factor alpha (TNF-α).
31782817	5	130	theme	factor	1006:1011	arg1	alpha					1013:1017	tumour necrosis factor alpha	990:1017	tumour necrosis factor alpha (TNF-α)	990:1025	SLP or SLP + gDNA pre-treatment significantly reduced the LPS-induced mRNA expression of tumour necrosis factor alpha (TNF-α).
31782817	14	131	theme	layer	2232:2236	arg1	DNA					2255:2257	genomic DNA	2247:2257	genomic DNA	2247:2257	This study investigated the effects of pre-treatments with different components such as surface layer protein, genomic DNA and unmethylated cytosine-phosphate-guanine-containing oligodeoxynucleotides, alone or in combination on immunomodulation, and evaluated the signalling mechanism in mouse macrophage RAW264.7 cells challenged with lipopolysaccharide.
31782817	14	131	theme	layer	2232:2236	arg1	oligodeoxynucleotides					2314:2334	unmethylated cytosine-phosphate-guanine-containing oligodeoxynucleotides	2263:2334	unmethylated cytosine-phosphate-guanine-containing oligodeoxynucleotides	2263:2334	This study investigated the effects of pre-treatments with different components such as surface layer protein, genomic DNA and unmethylated cytosine-phosphate-guanine-containing oligodeoxynucleotides, alone or in combination on immunomodulation, and evaluated the signalling mechanism in mouse macrophage RAW264.7 cells challenged with lipopolysaccharide.
31782817	14	131	theme	layer	2232:2236	arg1	protein					2238:2244	surface layer protein	2224:2244	surface layer protein	2224:2244	This study investigated the effects of pre-treatments with different components such as surface layer protein, genomic DNA and unmethylated cytosine-phosphate-guanine-containing oligodeoxynucleotides, alone or in combination on immunomodulation, and evaluated the signalling mechanism in mouse macrophage RAW264.7 cells challenged with lipopolysaccharide.
31782817	6	132	dep	combinations	1097:1108	arg1	SLP + CpG					1125:1133	SLP + CpG	1125:1133	SLP + CpG	1125:1133	Pre-treatment with LGG single components (SLP, gDNA or CpG) or their combinations (SLP + gDNA or SLP + CpG) significantly decreased the LPS-induced interleukin-6 (IL-6) mRNA level (P < 0·05).
31782817	6	132	dep	combinations	1097:1108	arg1	SLP + gDNA					1111:1120	SLP + gDNA	1111:1120	SLP + gDNA	1111:1120	Pre-treatment with LGG single components (SLP, gDNA or CpG) or their combinations (SLP + gDNA or SLP + CpG) significantly decreased the LPS-induced interleukin-6 (IL-6) mRNA level (P < 0·05).
31782817	6	132	dep	combinations	1097:1108	arg1	combinations					1097:1108	their combinations	1091:1108	their combinations (SLP + gDNA or SLP + CpG)	1091:1134	Pre-treatment with LGG single components (SLP, gDNA or CpG) or their combinations (SLP + gDNA or SLP + CpG) significantly decreased the LPS-induced interleukin-6 (IL-6) mRNA level (P < 0·05).
31782817	11	133	theme	LGG	1885:1887	arg1	components					1889:1898	nonviable probiotic LGG components	1865:1898	nonviable probiotic LGG components	1865:1898	These results indicate that nonviable probiotic LGG components exert an anti-inflammation effect on epithelial cells.
31782817	15	134	theme	protein	2664:2670	arg1	kinases					2672:2678	extracellular regulated protein kinases	2640:2678	extracellular regulated protein kinases	2640:2678	Pre-incubation with components alone or in combination generally inhibited the activation of Toll-like receptor, mitogen-activated protein kinases, extracellular regulated protein kinases and nuclear factor-kappa B signalling pathways in lipopolysaccharide-stimulated cells, which generally leads to attenuated inflammatory cytokine interleukin-6 and tumour necrosis factor alpha production.
31069125	2	0	theme	beet	473:476	arg1	pulp					478:481	sugar beet pulp	467:481	sugar beet pulp (rich in cellulose and pectin)	467:512	The purpose of this work has been to identify potential lignocellulolytic enzymes and to compare the secreted enzymes produced when the fungus is grown on sugar beet pulp (rich in cellulose and pectin) and corn cob (rich in cellulose and xylan).
31069125	5	1	from	function	788:795	arg1	degradation					804:814	the degradation	800:814	the degradation of the polysaccharides	800:837	The putative function in the degradation of the polysaccharides was determined using dbCAN software.
31069125	6	2	theme	sugar	1073:1077	arg1	pulp					1084:1087	sugar beet pulp	1073:1087	sugar beet pulp	1073:1087	The results show that there is a good correlation between the polysaccharide composition of the carbon sources and the function of the secreted enzymes: both cultures are rich in cellulases, while sugar beet pulp induces pectinases and corncob, xylanases.
31069125	0	3	theme	enzymes	70:76	arg1	enzymes					70:76	lignocellulolytic enzymes	52:76	lignocellulolytic enzymes	52:76	Corncob and sugar beet pulp induce specific sets of lignocellulolytic enzymes in Penicillium purpurogenum.
31069125	0	3	theme	enzymes	70:76	arg1	sets					44:47	specific sets	35:47	specific sets of lignocellulolytic enzymes	35:76	Corncob and sugar beet pulp induce specific sets of lignocellulolytic enzymes in Penicillium purpurogenum.
31069125	1	4	dep	cellulose	259:267	arg1	biodegradation					296:309	biodegradation	296:309	biodegradation	296:309	Penicillium purpurogenum is a filamentous fungus, which grows on a variety of natural carbon sources and secretes a large number of enzymes involved in cellulose, hemicelluloses and pectin biodegradation.
31069125	1	5	theme	enzymes	239:245	arg1	number					229:234	a large number	221:234	a large number of enzymes involved in cellulose, hemicelluloses and pectin biodegradation	221:309	Penicillium purpurogenum is a filamentous fungus, which grows on a variety of natural carbon sources and secretes a large number of enzymes involved in cellulose, hemicelluloses and pectin biodegradation.
31069125	6	6	from	rich	1047:1050	arg1	cellulases					1055:1064	cellulases	1055:1064	cellulases	1055:1064	The results show that there is a good correlation between the polysaccharide composition of the carbon sources and the function of the secreted enzymes: both cultures are rich in cellulases, while sugar beet pulp induces pectinases and corncob, xylanases.
31069125	6	7	theme	sources	979:985	arg1	function					995:1002	the function	991:1002	the function of the secreted enzymes	991:1026	The results show that there is a good correlation between the polysaccharide composition of the carbon sources and the function of the secreted enzymes: both cultures are rich in cellulases, while sugar beet pulp induces pectinases and corncob, xylanases.
31069125	6	7	theme	sources	979:985	arg1	composition					953:963	the polysaccharide composition	934:963	the polysaccharide composition of the carbon sources	934:985	The results show that there is a good correlation between the polysaccharide composition of the carbon sources and the function of the secreted enzymes: both cultures are rich in cellulases, while sugar beet pulp induces pectinases and corncob, xylanases.
31069125	4	8	from	supernatant	746:756	arg1	present					731:737	present	731:737	present	731:737	Using MASCOT and a genome-derived protein database, the proteins present in the supernatant were identified.
31069125	7	9	theme	better	1214:1219	arg1	understanding					1221:1233	a better understanding	1212:1233	a better understanding of the biodegradation process performed by the fungus	1212:1287	The eventual biochemical characterisation of these enzymes will be of value for a better understanding of the biodegradation process performed by the fungus and increase the availability of enzymes for biotechnological methods associated with this process.
31069125	2	10	from	rich	528:531	arg1	xylan					550:554	xylan	550:554	xylan	550:554	The purpose of this work has been to identify potential lignocellulolytic enzymes and to compare the secreted enzymes produced when the fungus is grown on sugar beet pulp (rich in cellulose and pectin) and corn cob (rich in cellulose and xylan).
31069125	2	10	from	rich	528:531	arg1	cellulose					536:544	cellulose	536:544	cellulose	536:544	The purpose of this work has been to identify potential lignocellulolytic enzymes and to compare the secreted enzymes produced when the fungus is grown on sugar beet pulp (rich in cellulose and pectin) and corn cob (rich in cellulose and xylan).
31069125	4	11	theme	protein	700:706	arg1	database					708:715	a genome-derived protein database	683:715	a genome-derived protein database	683:715	Using MASCOT and a genome-derived protein database, the proteins present in the supernatant were identified.
31069125	7	12	theme	biochemical	1145:1155	arg1	characterisation					1157:1172	The eventual biochemical characterisation	1132:1172	The eventual biochemical characterisation of these enzymes	1132:1189	The eventual biochemical characterisation of these enzymes will be of value for a better understanding of the biodegradation process performed by the fungus and increase the availability of enzymes for biotechnological methods associated with this process.
31069125	5	13	theme	polysaccharides	823:837	arg1	degradation					804:814	the degradation	800:814	the degradation of the polysaccharides	800:837	The putative function in the degradation of the polysaccharides was determined using dbCAN software.
31069125	2	14	theme	rich	484:487	arg1	pulp					478:481	sugar beet pulp	467:481	sugar beet pulp (rich in cellulose and pectin)	467:512	The purpose of this work has been to identify potential lignocellulolytic enzymes and to compare the secreted enzymes produced when the fungus is grown on sugar beet pulp (rich in cellulose and pectin) and corn cob (rich in cellulose and xylan).
31069125	4	15	link	genome-derived	685:698	arg1	database					708:715	a genome-derived protein database	683:715	a genome-derived protein database	683:715	Using MASCOT and a genome-derived protein database, the proteins present in the supernatant were identified.
31069125	5	16	theme	dbCAN	860:864	arg1	software					866:873	dbCAN software	860:873	dbCAN software	860:873	The putative function in the degradation of the polysaccharides was determined using dbCAN software.
31069125	2	17	theme	sugar	467:471	arg1	beet					473:476	sugar beet	467:476	sugar beet pulp (rich in cellulose and pectin)	467:512	The purpose of this work has been to identify potential lignocellulolytic enzymes and to compare the secreted enzymes produced when the fungus is grown on sugar beet pulp (rich in cellulose and pectin) and corn cob (rich in cellulose and xylan).
31069125	4	18	from	present	731:737	arg1	supernatant					746:756	the supernatant	742:756	the supernatant	742:756	Using MASCOT and a genome-derived protein database, the proteins present in the supernatant were identified.
31069125	7	19	theme	process	1257:1263	arg1	understanding					1221:1233	a better understanding	1212:1233	a better understanding of the biodegradation process performed by the fungus	1212:1287	The eventual biochemical characterisation of these enzymes will be of value for a better understanding of the biodegradation process performed by the fungus and increase the availability of enzymes for biotechnological methods associated with this process.
31069125	2	20	theme	lignocellulolytic	368:384	arg1	enzymes					386:392	potential lignocellulolytic enzymes	358:392	potential lignocellulolytic enzymes	358:392	The purpose of this work has been to identify potential lignocellulolytic enzymes and to compare the secreted enzymes produced when the fungus is grown on sugar beet pulp (rich in cellulose and pectin) and corn cob (rich in cellulose and xylan).
31069125	6	21	theme	carbon	972:977	arg1	sources					979:985	the carbon sources	968:985	the carbon sources	968:985	The results show that there is a good correlation between the polysaccharide composition of the carbon sources and the function of the secreted enzymes: both cultures are rich in cellulases, while sugar beet pulp induces pectinases and corncob, xylanases.
31069125	3	22	theme	mass	647:650	arg1	spectrometry					652:663	two-dimensional nano-liquid chromatography/tandem mass spectrometry	597:663	two-dimensional nano-liquid chromatography/tandem mass spectrometry	597:663	Culture supernatants were subjected to two-dimensional nano-liquid chromatography/tandem mass spectrometry.
31069125	6	23	dep	rich	1047:1050	arg1	cultures					1034:1041	both cultures	1029:1041	both cultures	1029:1041	The results show that there is a good correlation between the polysaccharide composition of the carbon sources and the function of the secreted enzymes: both cultures are rich in cellulases, while sugar beet pulp induces pectinases and corncob, xylanases.
31069125	6	23	dep	rich	1047:1050	arg1	xylanases					1121:1129	xylanases	1121:1129	xylanases	1121:1129	The results show that there is a good correlation between the polysaccharide composition of the carbon sources and the function of the secreted enzymes: both cultures are rich in cellulases, while sugar beet pulp induces pectinases and corncob, xylanases.
31069125	2	24	theme	potential	358:366	arg1	enzymes					386:392	potential lignocellulolytic enzymes	358:392	potential lignocellulolytic enzymes	358:392	The purpose of this work has been to identify potential lignocellulolytic enzymes and to compare the secreted enzymes produced when the fungus is grown on sugar beet pulp (rich in cellulose and pectin) and corn cob (rich in cellulose and xylan).
31069125	0	25	theme	beet	18:21	arg1	pulp					23:26	sugar beet pulp	12:26	sugar beet pulp	12:26	Corncob and sugar beet pulp induce specific sets of lignocellulolytic enzymes in Penicillium purpurogenum.
31069125	2	26	from	cellulose	536:544	arg1	rich					528:531	rich	528:531	rich	528:531	The purpose of this work has been to identify potential lignocellulolytic enzymes and to compare the secreted enzymes produced when the fungus is grown on sugar beet pulp (rich in cellulose and pectin) and corn cob (rich in cellulose and xylan).
31069125	2	27	theme	corn	518:521	arg1	cob					523:525	corn cob	518:525	corn cob (rich in cellulose and xylan)	518:555	The purpose of this work has been to identify potential lignocellulolytic enzymes and to compare the secreted enzymes produced when the fungus is grown on sugar beet pulp (rich in cellulose and pectin) and corn cob (rich in cellulose and xylan).
31069125	6	28	from	cellulases	1055:1064	arg1	rich					1047:1050	rich	1047:1050	rich	1047:1050	The results show that there is a good correlation between the polysaccharide composition of the carbon sources and the function of the secreted enzymes: both cultures are rich in cellulases, while sugar beet pulp induces pectinases and corncob, xylanases.
31069125	2	29	theme	secreted	413:420	arg1	enzymes					422:428	the secreted enzymes	409:428	the secreted enzymes produced when the fungus is grown on sugar beet pulp (rich in cellulose and pectin) and corn cob (rich in cellulose and xylan)	409:555	The purpose of this work has been to identify potential lignocellulolytic enzymes and to compare the secreted enzymes produced when the fungus is grown on sugar beet pulp (rich in cellulose and pectin) and corn cob (rich in cellulose and xylan).
31069125	0	30	theme	sugar	12:16	arg1	pulp					23:26	sugar beet pulp	12:26	sugar beet pulp	12:26	Corncob and sugar beet pulp induce specific sets of lignocellulolytic enzymes in Penicillium purpurogenum.
31069125	3	31	theme	two-dimensional	597:611	arg1	spectrometry					652:663	two-dimensional nano-liquid chromatography/tandem mass spectrometry	597:663	two-dimensional nano-liquid chromatography/tandem mass spectrometry	597:663	Culture supernatants were subjected to two-dimensional nano-liquid chromatography/tandem mass spectrometry.
31069125	4	32	theme	present	731:737	arg1	proteins					722:729	the proteins	718:729	the proteins present in the supernatant	718:756	Using MASCOT and a genome-derived protein database, the proteins present in the supernatant were identified.
31069125	1	33	theme	carbon	193:198	arg1	sources					200:206	natural carbon sources	185:206	natural carbon sources	185:206	Penicillium purpurogenum is a filamentous fungus, which grows on a variety of natural carbon sources and secretes a large number of enzymes involved in cellulose, hemicelluloses and pectin biodegradation.
31069125	2	34	from	xylan	550:554	arg1	rich					528:531	rich	528:531	rich	528:531	The purpose of this work has been to identify potential lignocellulolytic enzymes and to compare the secreted enzymes produced when the fungus is grown on sugar beet pulp (rich in cellulose and pectin) and corn cob (rich in cellulose and xylan).
31069125	3	35	theme	nano-liquid	613:623	arg1	spectrometry					652:663	two-dimensional nano-liquid chromatography/tandem mass spectrometry	597:663	two-dimensional nano-liquid chromatography/tandem mass spectrometry	597:663	Culture supernatants were subjected to two-dimensional nano-liquid chromatography/tandem mass spectrometry.
31069125	1	36	theme	sources	200:206	arg1	variety					174:180	a variety	172:180	a variety of natural carbon sources	172:206	Penicillium purpurogenum is a filamentous fungus, which grows on a variety of natural carbon sources and secretes a large number of enzymes involved in cellulose, hemicelluloses and pectin biodegradation.
31069125	1	36	theme	sources	200:206	arg1	sources					200:206	natural carbon sources	185:206	natural carbon sources	185:206	Penicillium purpurogenum is a filamentous fungus, which grows on a variety of natural carbon sources and secretes a large number of enzymes involved in cellulose, hemicelluloses and pectin biodegradation.
31069125	6	37	theme	polysaccharide	938:951	arg1	composition					953:963	the polysaccharide composition	934:963	the polysaccharide composition of the carbon sources	934:985	The results show that there is a good correlation between the polysaccharide composition of the carbon sources and the function of the secreted enzymes: both cultures are rich in cellulases, while sugar beet pulp induces pectinases and corncob, xylanases.
31069125	7	38	theme	eventual	1136:1143	arg1	characterisation					1157:1172	The eventual biochemical characterisation	1132:1172	The eventual biochemical characterisation of these enzymes	1132:1189	The eventual biochemical characterisation of these enzymes will be of value for a better understanding of the biodegradation process performed by the fungus and increase the availability of enzymes for biotechnological methods associated with this process.
31069125	7	39	theme	enzymes	1183:1189	arg1	characterisation					1157:1172	The eventual biochemical characterisation	1132:1172	The eventual biochemical characterisation of these enzymes	1132:1189	The eventual biochemical characterisation of these enzymes will be of value for a better understanding of the biodegradation process performed by the fungus and increase the availability of enzymes for biotechnological methods associated with this process.
31069125	5	40	theme	putative	779:786	arg1	function					788:795	The putative function	775:795	The putative function in the degradation of the polysaccharides	775:837	The putative function in the degradation of the polysaccharides was determined using dbCAN software.
31069125	2	41	theme	work	332:335	arg1	purpose					316:322	The purpose	312:322	The purpose of this work	312:335	The purpose of this work has been to identify potential lignocellulolytic enzymes and to compare the secreted enzymes produced when the fungus is grown on sugar beet pulp (rich in cellulose and pectin) and corn cob (rich in cellulose and xylan).
31069125	0	42	theme	specific	35:42	arg1	enzymes					70:76	lignocellulolytic enzymes	52:76	lignocellulolytic enzymes	52:76	Corncob and sugar beet pulp induce specific sets of lignocellulolytic enzymes in Penicillium purpurogenum.
31069125	0	42	theme	specific	35:42	arg1	sets					44:47	specific sets	35:47	specific sets of lignocellulolytic enzymes	35:76	Corncob and sugar beet pulp induce specific sets of lignocellulolytic enzymes in Penicillium purpurogenum.
31069125	4	43	attach	present	731:737	arg1	supernatant					746:756	the supernatant	742:756	the supernatant	742:756	Using MASCOT and a genome-derived protein database, the proteins present in the supernatant were identified.
31069125	4	43	attach	present	731:737	arg2	proteins					722:729	the proteins	718:729	the proteins present in the supernatant	718:756	Using MASCOT and a genome-derived protein database, the proteins present in the supernatant were identified.
31069125	2	44	theme	rich	528:531	arg1	cob					523:525	corn cob	518:525	corn cob (rich in cellulose and xylan)	518:555	The purpose of this work has been to identify potential lignocellulolytic enzymes and to compare the secreted enzymes produced when the fungus is grown on sugar beet pulp (rich in cellulose and pectin) and corn cob (rich in cellulose and xylan).
31069125	4	45	theme	genome-derived	685:698	arg1	database					708:715	a genome-derived protein database	683:715	a genome-derived protein database	683:715	Using MASCOT and a genome-derived protein database, the proteins present in the supernatant were identified.
31069125	7	46	theme	biotechnological	1334:1349	arg1	methods					1351:1357	biotechnological methods	1334:1357	biotechnological methods associated with this process	1334:1386	The eventual biochemical characterisation of these enzymes will be of value for a better understanding of the biodegradation process performed by the fungus and increase the availability of enzymes for biotechnological methods associated with this process.
31069125	2	47	from	rich	484:487	arg1	pectin					506:511	pectin	506:511	pectin	506:511	The purpose of this work has been to identify potential lignocellulolytic enzymes and to compare the secreted enzymes produced when the fungus is grown on sugar beet pulp (rich in cellulose and pectin) and corn cob (rich in cellulose and xylan).
31069125	2	47	from	rich	484:487	arg1	cellulose					492:500	cellulose	492:500	cellulose	492:500	The purpose of this work has been to identify potential lignocellulolytic enzymes and to compare the secreted enzymes produced when the fungus is grown on sugar beet pulp (rich in cellulose and pectin) and corn cob (rich in cellulose and xylan).
31069125	6	48	theme	secreted	1011:1018	arg1	enzymes					1020:1026	the secreted enzymes	1007:1026	the secreted enzymes	1007:1026	The results show that there is a good correlation between the polysaccharide composition of the carbon sources and the function of the secreted enzymes: both cultures are rich in cellulases, while sugar beet pulp induces pectinases and corncob, xylanases.
31069125	7	49	theme	biodegradation	1242:1255	arg1	process					1257:1263	the biodegradation process	1238:1263	the biodegradation process performed by the fungus	1238:1287	The eventual biochemical characterisation of these enzymes will be of value for a better understanding of the biodegradation process performed by the fungus and increase the availability of enzymes for biotechnological methods associated with this process.
31069125	6	50	theme	enzymes	1020:1026	arg1	function					995:1002	the function	991:1002	the function of the secreted enzymes	991:1026	The results show that there is a good correlation between the polysaccharide composition of the carbon sources and the function of the secreted enzymes: both cultures are rich in cellulases, while sugar beet pulp induces pectinases and corncob, xylanases.
31069125	6	50	theme	enzymes	1020:1026	arg1	composition					953:963	the polysaccharide composition	934:963	the polysaccharide composition of the carbon sources	934:985	The results show that there is a good correlation between the polysaccharide composition of the carbon sources and the function of the secreted enzymes: both cultures are rich in cellulases, while sugar beet pulp induces pectinases and corncob, xylanases.
31069125	3	51	theme	chromatography/tandem	625:645	arg1	spectrometry					652:663	two-dimensional nano-liquid chromatography/tandem mass spectrometry	597:663	two-dimensional nano-liquid chromatography/tandem mass spectrometry	597:663	Culture supernatants were subjected to two-dimensional nano-liquid chromatography/tandem mass spectrometry.
31069125	1	52	theme	filamentous	137:147	arg1	purpurogenum					119:130	Penicillium purpurogenum	107:130	Penicillium purpurogenum	107:130	Penicillium purpurogenum is a filamentous fungus, which grows on a variety of natural carbon sources and secretes a large number of enzymes involved in cellulose, hemicelluloses and pectin biodegradation.
31069125	1	52	theme	filamentous	137:147	arg1	fungus					149:154	a filamentous fungus	135:154	a filamentous fungus	135:154	Penicillium purpurogenum is a filamentous fungus, which grows on a variety of natural carbon sources and secretes a large number of enzymes involved in cellulose, hemicelluloses and pectin biodegradation.
31069125	2	53	from	pectin	506:511	arg1	rich					484:487	rich	484:487	rich	484:487	The purpose of this work has been to identify potential lignocellulolytic enzymes and to compare the secreted enzymes produced when the fungus is grown on sugar beet pulp (rich in cellulose and pectin) and corn cob (rich in cellulose and xylan).
31069125	0	54	theme	lignocellulolytic	52:68	arg1	enzymes					70:76	lignocellulolytic enzymes	52:76	lignocellulolytic enzymes	52:76	Corncob and sugar beet pulp induce specific sets of lignocellulolytic enzymes in Penicillium purpurogenum.
31069125	2	55	from	cellulose	492:500	arg1	rich					484:487	rich	484:487	rich	484:487	The purpose of this work has been to identify potential lignocellulolytic enzymes and to compare the secreted enzymes produced when the fungus is grown on sugar beet pulp (rich in cellulose and pectin) and corn cob (rich in cellulose and xylan).
31069125	1	56	theme	natural	185:191	arg1	sources					200:206	natural carbon sources	185:206	natural carbon sources	185:206	Penicillium purpurogenum is a filamentous fungus, which grows on a variety of natural carbon sources and secretes a large number of enzymes involved in cellulose, hemicelluloses and pectin biodegradation.
31069125	7	57	theme	enzymes	1322:1328	arg1	availability					1306:1317	the availability	1302:1317	the availability of enzymes for biotechnological methods associated with this process	1302:1386	The eventual biochemical characterisation of these enzymes will be of value for a better understanding of the biodegradation process performed by the fungus and increase the availability of enzymes for biotechnological methods associated with this process.
31069125	1	58	theme	large	223:227	arg1	number					229:234	a large number	221:234	a large number of enzymes involved in cellulose, hemicelluloses and pectin biodegradation	221:309	Penicillium purpurogenum is a filamentous fungus, which grows on a variety of natural carbon sources and secretes a large number of enzymes involved in cellulose, hemicelluloses and pectin biodegradation.
31069125	6	59	theme	good	909:912	arg1	correlation					914:924	a good correlation	907:924	a good correlation between the polysaccharide composition of the carbon sources and the function of the secreted enzymes	907:1026	The results show that there is a good correlation between the polysaccharide composition of the carbon sources and the function of the secreted enzymes: both cultures are rich in cellulases, while sugar beet pulp induces pectinases and corncob, xylanases.
31069125	3	60	theme	Culture	558:564	arg1	supernatants					566:577	Culture supernatants	558:577	Culture supernatants	558:577	Culture supernatants were subjected to two-dimensional nano-liquid chromatography/tandem mass spectrometry.
31069125	6	61	theme	beet	1079:1082	arg1	pulp					1084:1087	sugar beet pulp	1073:1087	sugar beet pulp	1073:1087	The results show that there is a good correlation between the polysaccharide composition of the carbon sources and the function of the secreted enzymes: both cultures are rich in cellulases, while sugar beet pulp induces pectinases and corncob, xylanases.
29803426	2	0	theme	milk	127:130	arg1	GMP					151:153	GMP	151:153	GMP	151:153	Bovine milk glycomacropeptide (GMP) is derived from κ-casein, with exclusively o-linked glycosylation.
29803426	2	0	theme	milk	127:130	arg1	glycomacropeptide					132:148	Bovine milk glycomacropeptide	120:148	Bovine milk glycomacropeptide (GMP)	120:154	Bovine milk glycomacropeptide (GMP) is derived from κ-casein, with exclusively o-linked glycosylation.
29803426	9	1	theme	glycoside	942:950	arg1	family					963:968	family 25	963:971	family 25	963:971	The pool of differentially expressed transcripts included 2 glycoside hydrolase (family 25) genes, which were substantially upregulated following exposure to GMP, but not GMP-P.
29803426	9	1	theme	glycoside	942:950	arg1	hydrolase					952:960	2 glycoside hydrolase	940:960	2 glycoside hydrolase (family 25) genes	940:978	The pool of differentially expressed transcripts included 2 glycoside hydrolase (family 25) genes, which were substantially upregulated following exposure to GMP, but not GMP-P.
29803426	8	2	theme	O-linked	858:865	arg1	glycosylation					867:879	the peptide's O-linked glycosylation	844:879	the peptide's O-linked glycosylation	844:879	infantis growth by GMP is intrinsically linked to the peptide's O-linked glycosylation.
29803426	10	3	theme	duplicated	1093:1102	arg1	islands					1112:1118	duplicated genomic islands	1093:1118	duplicated genomic islands that also contained genes encoding fibronectin type III binding domain proteins and numerous phage-related proteins, all of which were also upregulated	1093:1270	These GH25 genes were present in duplicated genomic islands that also contained genes encoding fibronectin type III binding domain proteins and numerous phage-related proteins, all of which were also upregulated.
29803426	2	4	theme	Bovine	120:125	arg1	GMP					151:153	GMP	151:153	GMP	151:153	Bovine milk glycomacropeptide (GMP) is derived from κ-casein, with exclusively o-linked glycosylation.
29803426	2	4	theme	Bovine	120:125	arg1	glycomacropeptide					132:148	Bovine milk glycomacropeptide	120:148	Bovine milk glycomacropeptide (GMP)	120:154	Bovine milk glycomacropeptide (GMP) is derived from κ-casein, with exclusively o-linked glycosylation.
29803426	12	5	theme	GMP	1540:1542	arg1	effect					1518:1523	the prebiotic effect	1504:1523	the prebiotic effect of bovine milk GMP on B. longum ssp	1504:1559	This study provides insights into the molecular basis for the prebiotic effect of bovine milk GMP on B. longum ssp.
29803426	12	6	theme	milk	1535:1538	arg1	GMP					1540:1542	bovine milk GMP	1528:1542	bovine milk GMP	1528:1542	This study provides insights into the molecular basis for the prebiotic effect of bovine milk GMP on B. longum ssp.
29803426	12	7	dep	B.	1547:1548	arg1	longum					1550:1555	longum	1550:1555	longum	1550:1555	This study provides insights into the molecular basis for the prebiotic effect of bovine milk GMP on B. longum ssp.
29803426	11	8	theme	arrangement	1298:1308	arg1	Homologs					1273:1280	Homologs	1273:1280	Homologs of this genomic arrangement	1273:1308	Homologs of this genomic arrangement were present in other Bifidobacterium species, which suggest it may be a conserved domain for the utilization of glycosylated peptides.
29803426	10	9	located	present	1082:1088	arg2	genes					1071:1075	These GH25 genes	1060:1075	These GH25 genes	1060:1075	These GH25 genes were present in duplicated genomic islands that also contained genes encoding fibronectin type III binding domain proteins and numerous phage-related proteins, all of which were also upregulated.
29803426	10	9	located	present	1082:1088	arg1	islands					1112:1118	duplicated genomic islands	1093:1118	duplicated genomic islands that also contained genes encoding fibronectin type III binding domain proteins and numerous phage-related proteins, all of which were also upregulated	1093:1270	These GH25 genes were present in duplicated genomic islands that also contained genes encoding fibronectin type III binding domain proteins and numerous phage-related proteins, all of which were also upregulated.
29803426	4	10	theme	concentration-dependent	306:328	arg1	manner					330:335	a concentration-dependent manner	304:335	a concentration-dependent manner	304:335	infantis in a concentration-dependent manner, and this activity was lost following periodate treatment of the GMP (GMP-P), which disables biological recognition of the conjugated oligosaccharides.
29803426	11	11	theme	glycosylated	1423:1434	arg1	peptides					1436:1443	glycosylated peptides	1423:1443	glycosylated peptides	1423:1443	Homologs of this genomic arrangement were present in other Bifidobacterium species, which suggest it may be a conserved domain for the utilization of glycosylated peptides.
29803426	5	12	theme	longum	520:525	arg1	analysis					505:512	Transcriptional analysis	489:512	Transcriptional analysis of B. longum	489:525	Transcriptional analysis of B. longum ssp.
29803426	11	13	gly	glycosylated	1423:1434	arg1	peptides					1436:1443	glycosylated peptides	1423:1443	glycosylated peptides	1423:1443	Homologs of this genomic arrangement were present in other Bifidobacterium species, which suggest it may be a conserved domain for the utilization of glycosylated peptides.
29803426	6	14	theme	substantial	578:588	arg1	response					590:597	a substantial response	576:597	a substantial response to GMP relative to bacteria treated with GMP-P	576:644	infantis following exposure to GMP revealed a substantial response to GMP relative to bacteria treated with GMP-P, with a greater number of differentially expressed transcripts and larger fold changes versus the control.
29803426	3	15	theme	longum	280:285	arg1	ssp					287:289	Bifidobacterium longum ssp	264:289	Bifidobacterium longum ssp	264:289	Glycomacropeptide promoted the growth of Bifidobacterium longum ssp.
29803426	10	16	theme	phage-related	1213:1225	arg1	proteins					1227:1234	numerous phage-related proteins	1204:1234	numerous phage-related proteins	1204:1234	These GH25 genes were present in duplicated genomic islands that also contained genes encoding fibronectin type III binding domain proteins and numerous phage-related proteins, all of which were also upregulated.
29803426	11	17	from	present	1315:1321	arg1	species					1348:1354	other Bifidobacterium species	1326:1354	other Bifidobacterium species	1326:1354	Homologs of this genomic arrangement were present in other Bifidobacterium species, which suggest it may be a conserved domain for the utilization of glycosylated peptides.
29803426	11	18	theme	other	1326:1330	arg1	species					1348:1354	other Bifidobacterium species	1326:1354	other Bifidobacterium species	1326:1354	Homologs of this genomic arrangement were present in other Bifidobacterium species, which suggest it may be a conserved domain for the utilization of glycosylated peptides.
29803426	11	19	theme	Bifidobacterium	1332:1346	arg1	species					1348:1354	other Bifidobacterium species	1326:1354	other Bifidobacterium species	1326:1354	Homologs of this genomic arrangement were present in other Bifidobacterium species, which suggest it may be a conserved domain for the utilization of glycosylated peptides.
29803426	0	20	theme	Bovine	0:5	arg1	glycomacropeptide					7:23	Bovine glycomacropeptide	0:23	Bovine glycomacropeptide	0:23	Bovine glycomacropeptide promotes the growth of Bifidobacterium longum ssp.
29803426	10	21	theme	type	1167:1170	arg1	proteins					1191:1198	fibronectin type III binding domain proteins	1155:1198	fibronectin type III binding domain proteins	1155:1198	These GH25 genes were present in duplicated genomic islands that also contained genes encoding fibronectin type III binding domain proteins and numerous phage-related proteins, all of which were also upregulated.
29803426	8	22	link	O-linked	858:865	arg1	glycosylation					867:879	the peptide's O-linked glycosylation	844:879	the peptide's O-linked glycosylation	844:879	infantis growth by GMP is intrinsically linked to the peptide's O-linked glycosylation.
29803426	12	23	theme	prebiotic	1508:1516	arg1	effect					1518:1523	the prebiotic effect	1504:1523	the prebiotic effect of bovine milk GMP on B. longum ssp	1504:1559	This study provides insights into the molecular basis for the prebiotic effect of bovine milk GMP on B. longum ssp.
29803426	5	24	theme	Transcriptional	489:503	arg1	analysis					505:512	Transcriptional analysis	489:512	Transcriptional analysis of B. longum	489:525	Transcriptional analysis of B. longum ssp.
29803426	12	25	theme	bovine	1528:1533	arg1	GMP					1540:1542	bovine milk GMP	1528:1542	bovine milk GMP	1528:1542	This study provides insights into the molecular basis for the prebiotic effect of bovine milk GMP on B. longum ssp.
29803426	10	26	theme	fibronectin	1155:1165	arg1	type					1167:1170	fibronectin type III	1155:1174	fibronectin type III binding domain proteins	1155:1198	These GH25 genes were present in duplicated genomic islands that also contained genes encoding fibronectin type III binding domain proteins and numerous phage-related proteins, all of which were also upregulated.
29803426	4	27	theme	GMP	402:404	arg1	treatment					385:393	periodate treatment	375:393	periodate treatment of the GMP (GMP-P), which disables biological recognition of the conjugated oligosaccharides	375:486	infantis in a concentration-dependent manner, and this activity was lost following periodate treatment of the GMP (GMP-P), which disables biological recognition of the conjugated oligosaccharides.
29803426	1	28	theme	gene	103:106	arg1	expression					108:117	its gene expression	99:117	its gene expression	99:117	infantis and modulates its gene expression.
29803426	6	29	theme	larger	713:718	arg1	changes					725:731	larger fold changes	713:731	larger fold changes	713:731	infantis following exposure to GMP revealed a substantial response to GMP relative to bacteria treated with GMP-P, with a greater number of differentially expressed transcripts and larger fold changes versus the control.
29803426	11	30	from	species	1348:1354	arg1	present					1315:1321	present	1315:1321	present	1315:1321	Homologs of this genomic arrangement were present in other Bifidobacterium species, which suggest it may be a conserved domain for the utilization of glycosylated peptides.
29803426	4	31	theme	conjugated	460:469	arg1	oligosaccharides					471:486	the conjugated oligosaccharides	456:486	the conjugated oligosaccharides	456:486	infantis in a concentration-dependent manner, and this activity was lost following periodate treatment of the GMP (GMP-P), which disables biological recognition of the conjugated oligosaccharides.
29803426	10	32	contain	contained	1130:1138	arg2	genes					1140:1144	genes	1140:1144	genes	1140:1144	These GH25 genes were present in duplicated genomic islands that also contained genes encoding fibronectin type III binding domain proteins and numerous phage-related proteins, all of which were also upregulated.
29803426	10	32	contain	contained	1130:1138	arg1	islands					1112:1118	duplicated genomic islands	1093:1118	duplicated genomic islands that also contained genes encoding fibronectin type III binding domain proteins and numerous phage-related proteins, all of which were also upregulated	1093:1270	These GH25 genes were present in duplicated genomic islands that also contained genes encoding fibronectin type III binding domain proteins and numerous phage-related proteins, all of which were also upregulated.
29803426	10	33	attach	present	1082:1088	arg2	genes					1071:1075	These GH25 genes	1060:1075	These GH25 genes	1060:1075	These GH25 genes were present in duplicated genomic islands that also contained genes encoding fibronectin type III binding domain proteins and numerous phage-related proteins, all of which were also upregulated.
29803426	10	33	attach	present	1082:1088	arg1	islands					1112:1118	duplicated genomic islands	1093:1118	duplicated genomic islands that also contained genes encoding fibronectin type III binding domain proteins and numerous phage-related proteins, all of which were also upregulated	1093:1270	These GH25 genes were present in duplicated genomic islands that also contained genes encoding fibronectin type III binding domain proteins and numerous phage-related proteins, all of which were also upregulated.
29803426	6	34	theme	fold	720:723	arg1	changes					725:731	larger fold changes	713:731	larger fold changes	713:731	infantis following exposure to GMP revealed a substantial response to GMP relative to bacteria treated with GMP-P, with a greater number of differentially expressed transcripts and larger fold changes versus the control.
29803426	11	35	theme	conserved	1383:1391	arg1	it					1371:1372	it	1371:1372	it	1371:1372	Homologs of this genomic arrangement were present in other Bifidobacterium species, which suggest it may be a conserved domain for the utilization of glycosylated peptides.
29803426	11	35	theme	conserved	1383:1391	arg1	domain					1393:1398	a conserved domain	1381:1398	a conserved domain for the utilization of glycosylated peptides	1381:1443	Homologs of this genomic arrangement were present in other Bifidobacterium species, which suggest it may be a conserved domain for the utilization of glycosylated peptides.
29803426	3	36	theme	ssp	287:289	arg1	growth					254:259	the growth	250:259	the growth of Bifidobacterium longum ssp	250:289	Glycomacropeptide promoted the growth of Bifidobacterium longum ssp.
29803426	7	37	dep	B.	779:780	arg1	longum					782:787	longum	782:787	longum	782:787	Therefore, stimulation of B. longum ssp.
29803426	6	38	theme	transcripts	697:707	arg1	changes					725:731	larger fold changes	713:731	larger fold changes	713:731	infantis following exposure to GMP revealed a substantial response to GMP relative to bacteria treated with GMP-P, with a greater number of differentially expressed transcripts and larger fold changes versus the control.
29803426	6	38	theme	transcripts	697:707	arg1	number					662:667	a greater number	652:667	a greater number of differentially expressed transcripts	652:707	infantis following exposure to GMP revealed a substantial response to GMP relative to bacteria treated with GMP-P, with a greater number of differentially expressed transcripts and larger fold changes versus the control.
29803426	6	38	theme	transcripts	697:707	arg1	control					744:750	the control	740:750	the control	740:750	infantis following exposure to GMP revealed a substantial response to GMP relative to bacteria treated with GMP-P, with a greater number of differentially expressed transcripts and larger fold changes versus the control.
29803426	2	39	attach	derived	159:165	arg1	κ-casein					172:179	κ-casein	172:179	κ-casein	172:179	Bovine milk glycomacropeptide (GMP) is derived from κ-casein, with exclusively o-linked glycosylation.
29803426	2	39	attach	derived	159:165	arg2	GMP					151:153	GMP	151:153	GMP	151:153	Bovine milk glycomacropeptide (GMP) is derived from κ-casein, with exclusively o-linked glycosylation.
29803426	2	39	attach	derived	159:165	arg2	glycomacropeptide					132:148	Bovine milk glycomacropeptide	120:148	Bovine milk glycomacropeptide (GMP)	120:154	Bovine milk glycomacropeptide (GMP) is derived from κ-casein, with exclusively o-linked glycosylation.
29803426	10	40	from	islands	1112:1118	arg1	present					1082:1088	present	1082:1088	present	1082:1088	These GH25 genes were present in duplicated genomic islands that also contained genes encoding fibronectin type III binding domain proteins and numerous phage-related proteins, all of which were also upregulated.
29803426	9	41	theme	expressed	909:917	arg1	transcripts					919:929	differentially expressed transcripts	894:929	differentially expressed transcripts	894:929	The pool of differentially expressed transcripts included 2 glycoside hydrolase (family 25) genes, which were substantially upregulated following exposure to GMP, but not GMP-P.
29803426	12	42	theme	B.	1547:1548	arg1	ssp					1557:1559	B. longum ssp	1547:1559	B. longum ssp	1547:1559	This study provides insights into the molecular basis for the prebiotic effect of bovine milk GMP on B. longum ssp.
29803426	11	43	theme	peptides	1436:1443	arg1	utilization					1408:1418	the utilization	1404:1418	the utilization of glycosylated peptides	1404:1443	Homologs of this genomic arrangement were present in other Bifidobacterium species, which suggest it may be a conserved domain for the utilization of glycosylated peptides.
29803426	2	44	link	o-linked	199:206	arg1	glycosylation					208:220	o-linked glycosylation	199:220	o-linked glycosylation	199:220	Bovine milk glycomacropeptide (GMP) is derived from κ-casein, with exclusively o-linked glycosylation.
29803426	0	45	theme	longum	64:69	arg1	ssp					71:73	Bifidobacterium longum ssp	48:73	Bifidobacterium longum ssp	48:73	Bovine glycomacropeptide promotes the growth of Bifidobacterium longum ssp.
29803426	10	46	theme	domain	1184:1189	arg1	proteins					1191:1198	fibronectin type III binding domain proteins	1155:1198	fibronectin type III binding domain proteins	1155:1198	These GH25 genes were present in duplicated genomic islands that also contained genes encoding fibronectin type III binding domain proteins and numerous phage-related proteins, all of which were also upregulated.
29803426	9	47	theme	transcripts	919:929	arg1	pool					886:889	The pool	882:889	The pool of differentially expressed transcripts	882:929	The pool of differentially expressed transcripts included 2 glycoside hydrolase (family 25) genes, which were substantially upregulated following exposure to GMP, but not GMP-P.
29803426	4	48	theme	oligosaccharides	471:486	arg1	recognition					441:451	biological recognition	430:451	biological recognition of the conjugated oligosaccharides	430:486	infantis in a concentration-dependent manner, and this activity was lost following periodate treatment of the GMP (GMP-P), which disables biological recognition of the conjugated oligosaccharides.
29803426	4	49	dep	infantis	292:299	arg1	manner					330:335	a concentration-dependent manner	304:335	a concentration-dependent manner	304:335	infantis in a concentration-dependent manner, and this activity was lost following periodate treatment of the GMP (GMP-P), which disables biological recognition of the conjugated oligosaccharides.
29803426	10	50	theme	binding	1176:1182	arg1	proteins					1191:1198	fibronectin type III binding domain proteins	1155:1198	fibronectin type III binding domain proteins	1155:1198	These GH25 genes were present in duplicated genomic islands that also contained genes encoding fibronectin type III binding domain proteins and numerous phage-related proteins, all of which were also upregulated.
29803426	11	51	theme	genomic	1290:1296	arg1	arrangement					1298:1308	this genomic arrangement	1285:1308	this genomic arrangement	1285:1308	Homologs of this genomic arrangement were present in other Bifidobacterium species, which suggest it may be a conserved domain for the utilization of glycosylated peptides.
29803426	4	52	theme	biological	430:439	arg1	recognition					441:451	biological recognition	430:451	biological recognition of the conjugated oligosaccharides	430:486	infantis in a concentration-dependent manner, and this activity was lost following periodate treatment of the GMP (GMP-P), which disables biological recognition of the conjugated oligosaccharides.
29803426	6	53	theme	expressed	687:695	arg1	transcripts					697:707	differentially expressed transcripts	672:707	differentially expressed transcripts	672:707	infantis following exposure to GMP revealed a substantial response to GMP relative to bacteria treated with GMP-P, with a greater number of differentially expressed transcripts and larger fold changes versus the control.
29803426	2	54	theme	o-linked	199:206	arg1	glycosylation					208:220	o-linked glycosylation	199:220	o-linked glycosylation	199:220	Bovine milk glycomacropeptide (GMP) is derived from κ-casein, with exclusively o-linked glycosylation.
29803426	4	55	theme	periodate	375:383	arg1	treatment					385:393	periodate treatment	375:393	periodate treatment of the GMP (GMP-P), which disables biological recognition of the conjugated oligosaccharides	375:486	infantis in a concentration-dependent manner, and this activity was lost following periodate treatment of the GMP (GMP-P), which disables biological recognition of the conjugated oligosaccharides.
29803426	12	56	theme	molecular	1484:1492	arg1	basis					1494:1498	the molecular basis	1480:1498	the molecular basis for the prebiotic effect of bovine milk GMP on B. longum ssp	1480:1559	This study provides insights into the molecular basis for the prebiotic effect of bovine milk GMP on B. longum ssp.
29803426	10	57	from	present	1082:1088	arg1	islands					1112:1118	duplicated genomic islands	1093:1118	duplicated genomic islands that also contained genes encoding fibronectin type III binding domain proteins and numerous phage-related proteins, all of which were also upregulated	1093:1270	These GH25 genes were present in duplicated genomic islands that also contained genes encoding fibronectin type III binding domain proteins and numerous phage-related proteins, all of which were also upregulated.
29803426	8	58	theme	infantis	794:801	arg1	growth					803:808	infantis growth	794:808	infantis growth by GMP	794:815	infantis growth by GMP is intrinsically linked to the peptide's O-linked glycosylation.
29803426	5	59	dep	ssp	527:529	arg1	analysis					505:512	Transcriptional analysis	489:512	Transcriptional analysis of B. longum	489:525	Transcriptional analysis of B. longum ssp.
29803426	12	60	from	effect	1518:1523	arg1	ssp					1557:1559	B. longum ssp	1547:1559	B. longum ssp	1547:1559	This study provides insights into the molecular basis for the prebiotic effect of bovine milk GMP on B. longum ssp.
29803426	11	61	located	present	1315:1321	arg1	species					1348:1354	other Bifidobacterium species	1326:1354	other Bifidobacterium species	1326:1354	Homologs of this genomic arrangement were present in other Bifidobacterium species, which suggest it may be a conserved domain for the utilization of glycosylated peptides.
29803426	11	61	located	present	1315:1321	arg2	Homologs					1273:1280	Homologs	1273:1280	Homologs of this genomic arrangement	1273:1308	Homologs of this genomic arrangement were present in other Bifidobacterium species, which suggest it may be a conserved domain for the utilization of glycosylated peptides.
29803426	7	62	theme	ssp	789:791	arg1	stimulation					764:774	stimulation	764:774	stimulation of B. longum ssp	764:791	Therefore, stimulation of B. longum ssp.
29803426	9	63	theme	hydrolase	952:960	arg1	genes					974:978	2 glycoside hydrolase (family 25) genes	940:978	2 glycoside hydrolase (family 25) genes	940:978	The pool of differentially expressed transcripts included 2 glycoside hydrolase (family 25) genes, which were substantially upregulated following exposure to GMP, but not GMP-P.
29803426	10	64	theme	numerous	1204:1211	arg1	proteins					1227:1234	numerous phage-related proteins	1204:1234	numerous phage-related proteins	1204:1234	These GH25 genes were present in duplicated genomic islands that also contained genes encoding fibronectin type III binding domain proteins and numerous phage-related proteins, all of which were also upregulated.
29803426	0	65	theme	ssp	71:73	arg1	growth					38:43	the growth	34:43	the growth of Bifidobacterium longum ssp	34:73	Bovine glycomacropeptide promotes the growth of Bifidobacterium longum ssp.
29803426	10	66	theme	genomic	1104:1110	arg1	islands					1112:1118	duplicated genomic islands	1093:1118	duplicated genomic islands that also contained genes encoding fibronectin type III binding domain proteins and numerous phage-related proteins, all of which were also upregulated	1093:1270	These GH25 genes were present in duplicated genomic islands that also contained genes encoding fibronectin type III binding domain proteins and numerous phage-related proteins, all of which were also upregulated.
29803426	11	67	attach	present	1315:1321	arg1	species					1348:1354	other Bifidobacterium species	1326:1354	other Bifidobacterium species	1326:1354	Homologs of this genomic arrangement were present in other Bifidobacterium species, which suggest it may be a conserved domain for the utilization of glycosylated peptides.
29803426	11	67	attach	present	1315:1321	arg2	Homologs					1273:1280	Homologs	1273:1280	Homologs of this genomic arrangement	1273:1308	Homologs of this genomic arrangement were present in other Bifidobacterium species, which suggest it may be a conserved domain for the utilization of glycosylated peptides.
29803426	6	68	theme	greater	654:660	arg1	number					662:667	a greater number	652:667	a greater number of differentially expressed transcripts	652:707	infantis following exposure to GMP revealed a substantial response to GMP relative to bacteria treated with GMP-P, with a greater number of differentially expressed transcripts and larger fold changes versus the control.
29803426	7	69	theme	B.	779:780	arg1	ssp					789:791	B. longum ssp	779:791	B. longum ssp	779:791	Therefore, stimulation of B. longum ssp.
30984549	2	0	theme	cirrhosis	546:554	arg1	progression					525:535	progression	525:535	progression of liver cirrhosis	525:554	If it is possible to control the modification of oligosaccharides on VN, it may be possible to retard progression of liver cirrhosis.
30984549	1	1	from	remodeling	392:401	arg1	cirrhosis					412:420	liver cirrhosis	406:420	liver cirrhosis	406:420	Liver cirrhosis (LC) is a disease characterized by pathological accumulation and alteration of extracellular matrix (ECM) proteins; the interaction between two such proteins, collagen and vitronectin (VN), is considered to be the key to controlling ECM remodeling in liver cirrhosis.
30984549	4	2	theme	human	804:808	arg1	LC					810:811	human LC	804:811	human LC	804:811	Plasma concentrations of VN in human LC declined to approximately two-thirds that in normal plasma, but the ratio of active VN, which has collagen-binding activities, increased 2.8 times in LC plasma.
30984549	3	3	theme	VN	620:621	arg1	glycosylation					623:635	VN glycosylation	620:635	VN glycosylation	620:635	In this study, we examined the relationship between changes in VN glycosylation and activity related to the remodeling of hepatic tissue in human LC and a rat model of LC generated using carbon tetrachloride (CCl4).
30984549	9	4	from	composition	1631:1641	arg1	levels					1551:1556	plasma levels	1544:1556	plasma levels of human VN	1544:1568	The changes in rat VN due to CCl4 treatment did not correspond to the changes in plasma levels of human VN caused by LC, the ratio of active molecules, or carbohydrate composition, thereby indicating that CCl4-treated rats are not an appropriate model for studying VNs in human LC.
30984549	4	5	theme	VN	798:799	arg1	concentrations					780:793	Plasma concentrations	773:793	Plasma concentrations of VN in human LC	773:811	Plasma concentrations of VN in human LC declined to approximately two-thirds that in normal plasma, but the ratio of active VN, which has collagen-binding activities, increased 2.8 times in LC plasma.
30984549	7	6	theme	plasma	1280:1285	arg1	level					1287:1291	the plasma level	1276:1291	the plasma level of rat CCl4-VN	1276:1306	On the other hand, the plasma level of rat CCl4-VN increased and the ratio of active molecules to collagen in plasma decreased.
30984549	9	7	theme	appropriate	1697:1707	arg1	model					1709:1713	an appropriate model	1694:1713	an appropriate model for studying VNs in human LC	1694:1742	The changes in rat VN due to CCl4 treatment did not correspond to the changes in plasma levels of human VN caused by LC, the ratio of active molecules, or carbohydrate composition, thereby indicating that CCl4-treated rats are not an appropriate model for studying VNs in human LC.
30984549	9	7	theme	appropriate	1697:1707	arg1	rats					1681:1684	CCl4-treated rats	1668:1684	CCl4-treated rats	1668:1684	The changes in rat VN due to CCl4 treatment did not correspond to the changes in plasma levels of human VN caused by LC, the ratio of active molecules, or carbohydrate composition, thereby indicating that CCl4-treated rats are not an appropriate model for studying VNs in human LC.
30984549	1	8	theme	liver	406:410	arg1	cirrhosis					412:420	liver cirrhosis	406:420	liver cirrhosis	406:420	Liver cirrhosis (LC) is a disease characterized by pathological accumulation and alteration of extracellular matrix (ECM) proteins; the interaction between two such proteins, collagen and vitronectin (VN), is considered to be the key to controlling ECM remodeling in liver cirrhosis.
30984549	1	9	theme	proteins	261:268	arg1	alteration					220:229	alteration	220:229	alteration	220:229	Liver cirrhosis (LC) is a disease characterized by pathological accumulation and alteration of extracellular matrix (ECM) proteins; the interaction between two such proteins, collagen and vitronectin (VN), is considered to be the key to controlling ECM remodeling in liver cirrhosis.
30984549	1	9	theme	proteins	261:268	arg1	accumulation					203:214	pathological accumulation	190:214	pathological accumulation	190:214	Liver cirrhosis (LC) is a disease characterized by pathological accumulation and alteration of extracellular matrix (ECM) proteins; the interaction between two such proteins, collagen and vitronectin (VN), is considered to be the key to controlling ECM remodeling in liver cirrhosis.
30984549	3	10	theme	related	650:656	arg1	glycosylation					623:635	VN glycosylation	620:635	VN glycosylation	620:635	In this study, we examined the relationship between changes in VN glycosylation and activity related to the remodeling of hepatic tissue in human LC and a rat model of LC generated using carbon tetrachloride (CCl4).
30984549	6	11	theme	carbohydrate	1121:1132	arg1	analyses					1134:1141	carbohydrate analyses	1121:1141	carbohydrate analyses of LC-VN	1121:1150	Lectin reactivities and carbohydrate analyses of LC-VN revealed that branching, fucosylation, and sialylation of N-glycans were higher than those of normal VN.
30984549	7	12	theme	rat	1296:1298	arg1	CCl4-VN					1300:1306	rat CCl4-VN	1296:1306	rat CCl4-VN	1296:1306	On the other hand, the plasma level of rat CCl4-VN increased and the ratio of active molecules to collagen in plasma decreased.
30984549	7	13	theme	other	1264:1268	arg1	hand					1270:1273	the other hand	1260:1273	the other hand	1260:1273	On the other hand, the plasma level of rat CCl4-VN increased and the ratio of active molecules to collagen in plasma decreased.
30984549	3	14	theme	tissue	687:692	arg1	remodeling					665:674	the remodeling	661:674	the remodeling of hepatic tissue in human LC	661:704	In this study, we examined the relationship between changes in VN glycosylation and activity related to the remodeling of hepatic tissue in human LC and a rat model of LC generated using carbon tetrachloride (CCl4).
30984549	4	15	theme	Plasma	773:778	arg1	concentrations					780:793	Plasma concentrations	773:793	Plasma concentrations of VN in human LC	773:811	Plasma concentrations of VN in human LC declined to approximately two-thirds that in normal plasma, but the ratio of active VN, which has collagen-binding activities, increased 2.8 times in LC plasma.
30984549	9	16	theme	human	1735:1739	arg1	LC					1741:1742	human LC	1735:1742	human LC	1735:1742	The changes in rat VN due to CCl4 treatment did not correspond to the changes in plasma levels of human VN caused by LC, the ratio of active molecules, or carbohydrate composition, thereby indicating that CCl4-treated rats are not an appropriate model for studying VNs in human LC.
30984549	3	17	from	remodeling	665:674	arg1	LC					703:704	human LC	697:704	human LC	697:704	In this study, we examined the relationship between changes in VN glycosylation and activity related to the remodeling of hepatic tissue in human LC and a rat model of LC generated using carbon tetrachloride (CCl4).
30984549	0	18	theme	remodeling	118:127	arg1	activity					129:136	tissue remodeling activity	111:136	tissue remodeling activity	111:136	Vitronectins produced by human cirrhotic liver and CCl4-treated rats differ in their glycosylation pattern and tissue remodeling activity.
30984549	1	19	theme	pathological	190:201	arg1	accumulation					203:214	pathological accumulation	190:214	pathological accumulation	190:214	Liver cirrhosis (LC) is a disease characterized by pathological accumulation and alteration of extracellular matrix (ECM) proteins; the interaction between two such proteins, collagen and vitronectin (VN), is considered to be the key to controlling ECM remodeling in liver cirrhosis.
30984549	6	20	theme	Lectin	1097:1102	arg1	reactivities					1104:1115	Lectin reactivities	1097:1115	Lectin reactivities	1097:1115	Lectin reactivities and carbohydrate analyses of LC-VN revealed that branching, fucosylation, and sialylation of N-glycans were higher than those of normal VN.
30984549	6	21	gly	sialylation	1195:1205	arg1	N-glycans					1210:1218	N-glycans	1210:1218	N-glycans	1210:1218	Lectin reactivities and carbohydrate analyses of LC-VN revealed that branching, fucosylation, and sialylation of N-glycans were higher than those of normal VN.
30984549	7	22	from	ratio	1326:1330	arg1	plasma					1367:1372	plasma	1367:1372	plasma	1367:1372	On the other hand, the plasma level of rat CCl4-VN increased and the ratio of active molecules to collagen in plasma decreased.
30984549	9	23	theme	plasma	1544:1549	arg1	levels					1551:1556	plasma levels	1544:1556	plasma levels of human VN	1544:1568	The changes in rat VN due to CCl4 treatment did not correspond to the changes in plasma levels of human VN caused by LC, the ratio of active molecules, or carbohydrate composition, thereby indicating that CCl4-treated rats are not an appropriate model for studying VNs in human LC.
30984549	3	24	theme	human	697:701	arg1	LC					703:704	human LC	697:704	human LC	697:704	In this study, we examined the relationship between changes in VN glycosylation and activity related to the remodeling of hepatic tissue in human LC and a rat model of LC generated using carbon tetrachloride (CCl4).
30984549	2	25	from	modification	456:467	arg1	VN					492:493	VN	492:493	VN	492:493	If it is possible to control the modification of oligosaccharides on VN, it may be possible to retard progression of liver cirrhosis.
30984549	1	26	theme	Liver	139:143	arg1	disease					165:171	a disease	163:171	a disease characterized by pathological accumulation and alteration of extracellular matrix (ECM) proteins	163:268	Liver cirrhosis (LC) is a disease characterized by pathological accumulation and alteration of extracellular matrix (ECM) proteins; the interaction between two such proteins, collagen and vitronectin (VN), is considered to be the key to controlling ECM remodeling in liver cirrhosis.
30984549	1	26	theme	Liver	139:143	arg1	LC					156:157	LC	156:157	LC	156:157	Liver cirrhosis (LC) is a disease characterized by pathological accumulation and alteration of extracellular matrix (ECM) proteins; the interaction between two such proteins, collagen and vitronectin (VN), is considered to be the key to controlling ECM remodeling in liver cirrhosis.
30984549	1	26	theme	Liver	139:143	arg1	cirrhosis					145:153	Liver cirrhosis	139:153	Liver cirrhosis (LC)	139:158	Liver cirrhosis (LC) is a disease characterized by pathological accumulation and alteration of extracellular matrix (ECM) proteins; the interaction between two such proteins, collagen and vitronectin (VN), is considered to be the key to controlling ECM remodeling in liver cirrhosis.
30984549	9	27	theme	VN	1567:1568	arg1	levels					1551:1556	plasma levels	1544:1556	plasma levels of human VN	1544:1568	The changes in rat VN due to CCl4 treatment did not correspond to the changes in plasma levels of human VN caused by LC, the ratio of active molecules, or carbohydrate composition, thereby indicating that CCl4-treated rats are not an appropriate model for studying VNs in human LC.
30984549	2	28	from	oligosaccharides	472:487	arg1	VN					492:493	VN	492:493	VN	492:493	If it is possible to control the modification of oligosaccharides on VN, it may be possible to retard progression of liver cirrhosis.
30984549	10	29	theme	tissue	1963:1968	arg1	fibrosis					1970:1977	(b) tissue fibrosis	1959:1977	(b) tissue fibrosis	1959:1977	Glycosidase treatment of VNs supported the hypothesis that the collagen-binding activity of VN is modulated by alterations of glycosylation during LC, which may contribute to (a) the matrix incorporation of VN and (b) tissue fibrosis.
30984549	3	30	theme	rat	712:714	arg1	model					716:720	a rat model	710:720	a rat model of LC generated using carbon tetrachloride (CCl4)	710:770	In this study, we examined the relationship between changes in VN glycosylation and activity related to the remodeling of hepatic tissue in human LC and a rat model of LC generated using carbon tetrachloride (CCl4).
30984549	3	31	theme	carbon	744:749	arg1	CCl4					766:769	CCl4	766:769	CCl4	766:769	In this study, we examined the relationship between changes in VN glycosylation and activity related to the remodeling of hepatic tissue in human LC and a rat model of LC generated using carbon tetrachloride (CCl4).
30984549	3	31	theme	carbon	744:749	arg1	tetrachloride					751:763	carbon tetrachloride	744:763	carbon tetrachloride (CCl4)	744:770	In this study, we examined the relationship between changes in VN glycosylation and activity related to the remodeling of hepatic tissue in human LC and a rat model of LC generated using carbon tetrachloride (CCl4).
30984549	4	32	from	concentrations	780:793	arg1	LC					810:811	human LC	804:811	human LC	804:811	Plasma concentrations of VN in human LC declined to approximately two-thirds that in normal plasma, but the ratio of active VN, which has collagen-binding activities, increased 2.8 times in LC plasma.
30984549	4	33	theme	normal	858:863	arg1	plasma					865:870	normal plasma	858:870	normal plasma	858:870	Plasma concentrations of VN in human LC declined to approximately two-thirds that in normal plasma, but the ratio of active VN, which has collagen-binding activities, increased 2.8 times in LC plasma.
30984549	4	34	dep	two-thirds	839:848	arg1	that					850:853	that	850:853	that	850:853	Plasma concentrations of VN in human LC declined to approximately two-thirds that in normal plasma, but the ratio of active VN, which has collagen-binding activities, increased 2.8 times in LC plasma.
30984549	8	35	gly	fucosylation	1395:1406	arg1	carbohydrates					1437:1449	carbohydrates	1437:1449	carbohydrates of CCl4-VN	1437:1460	Increased fucosylation of LC-VN was not detected in carbohydrates of CCl4-VN.
30984549	8	35	gly	fucosylation	1395:1406	arg1	LC-VN					1411:1415	LC-VN	1411:1415	LC-VN	1411:1415	Increased fucosylation of LC-VN was not detected in carbohydrates of CCl4-VN.
30984549	5	36	theme	purified	987:994	arg1	LC-VN					996:1000	purified LC-VN	987:1000	purified LC-VN	987:1000	In contrast, purified LC-VN exhibited similar binding activities toward type I, IV, and V collagens to those of normal VN.
30984549	9	37	theme	carbohydrate	1618:1629	arg1	composition					1631:1641	carbohydrate composition	1618:1641	carbohydrate composition	1618:1641	The changes in rat VN due to CCl4 treatment did not correspond to the changes in plasma levels of human VN caused by LC, the ratio of active molecules, or carbohydrate composition, thereby indicating that CCl4-treated rats are not an appropriate model for studying VNs in human LC.
30984549	10	38	dep	fibrosis	1970:1977	arg1	b					1960:1960	b	1960:1960	b	1960:1960	Glycosidase treatment of VNs supported the hypothesis that the collagen-binding activity of VN is modulated by alterations of glycosylation during LC, which may contribute to (a) the matrix incorporation of VN and (b) tissue fibrosis.
30984549	4	39	theme	collagen-binding	911:926	arg1	activities					928:937	collagen-binding activities	911:937	collagen-binding activities	911:937	Plasma concentrations of VN in human LC declined to approximately two-thirds that in normal plasma, but the ratio of active VN, which has collagen-binding activities, increased 2.8 times in LC plasma.
30984549	8	40	theme	Increased	1385:1393	arg1	fucosylation					1395:1406	Increased fucosylation	1385:1406	Increased fucosylation of LC-VN	1385:1415	Increased fucosylation of LC-VN was not detected in carbohydrates of CCl4-VN.
30984549	9	41	from	ratio	1588:1592	arg1	levels					1551:1556	plasma levels	1544:1556	plasma levels of human VN	1544:1568	The changes in rat VN due to CCl4 treatment did not correspond to the changes in plasma levels of human VN caused by LC, the ratio of active molecules, or carbohydrate composition, thereby indicating that CCl4-treated rats are not an appropriate model for studying VNs in human LC.
30984549	4	42	theme	VN	897:898	arg1	ratio					881:885	the ratio	877:885	the ratio	877:885	Plasma concentrations of VN in human LC declined to approximately two-thirds that in normal plasma, but the ratio of active VN, which has collagen-binding activities, increased 2.8 times in LC plasma.
30984549	0	43	theme	cirrhotic	31:39	arg1	liver					41:45	human cirrhotic liver	25:45	human cirrhotic liver	25:45	Vitronectins produced by human cirrhotic liver and CCl4-treated rats differ in their glycosylation pattern and tissue remodeling activity.
30984549	10	44	theme	collagen-binding	1808:1823	arg1	activity					1825:1832	the collagen-binding activity	1804:1832	the collagen-binding activity of VN	1804:1838	Glycosidase treatment of VNs supported the hypothesis that the collagen-binding activity of VN is modulated by alterations of glycosylation during LC, which may contribute to (a) the matrix incorporation of VN and (b) tissue fibrosis.
30984549	10	45	dep	incorporation	1935:1947	arg1	a					1921:1921	a	1921:1921	a	1921:1921	Glycosidase treatment of VNs supported the hypothesis that the collagen-binding activity of VN is modulated by alterations of glycosylation during LC, which may contribute to (a) the matrix incorporation of VN and (b) tissue fibrosis.
30984549	5	46	theme	normal	1086:1091	arg1	VN					1093:1094	normal VN	1086:1094	normal VN	1086:1094	In contrast, purified LC-VN exhibited similar binding activities toward type I, IV, and V collagens to those of normal VN.
30984549	7	47	theme	active	1335:1340	arg1	molecules					1342:1350	active molecules	1335:1350	active molecules	1335:1350	On the other hand, the plasma level of rat CCl4-VN increased and the ratio of active molecules to collagen in plasma decreased.
30984549	9	48	theme	CCl4-treated	1668:1679	arg1	model					1709:1713	an appropriate model	1694:1713	an appropriate model for studying VNs in human LC	1694:1742	The changes in rat VN due to CCl4 treatment did not correspond to the changes in plasma levels of human VN caused by LC, the ratio of active molecules, or carbohydrate composition, thereby indicating that CCl4-treated rats are not an appropriate model for studying VNs in human LC.
30984549	9	48	theme	CCl4-treated	1668:1679	arg1	rats					1681:1684	CCl4-treated rats	1668:1684	CCl4-treated rats	1668:1684	The changes in rat VN due to CCl4 treatment did not correspond to the changes in plasma levels of human VN caused by LC, the ratio of active molecules, or carbohydrate composition, thereby indicating that CCl4-treated rats are not an appropriate model for studying VNs in human LC.
30984549	2	49	from	VN	492:493	arg1	modification					456:467	the modification	452:467	the modification of oligosaccharides on VN	452:493	If it is possible to control the modification of oligosaccharides on VN, it may be possible to retard progression of liver cirrhosis.
30984549	1	50	theme	extracellular	234:246	arg1	ECM					256:258	ECM	256:258	ECM	256:258	Liver cirrhosis (LC) is a disease characterized by pathological accumulation and alteration of extracellular matrix (ECM) proteins; the interaction between two such proteins, collagen and vitronectin (VN), is considered to be the key to controlling ECM remodeling in liver cirrhosis.
30984549	1	50	theme	extracellular	234:246	arg1	matrix					248:253	extracellular matrix	234:253	extracellular matrix (ECM) proteins	234:268	Liver cirrhosis (LC) is a disease characterized by pathological accumulation and alteration of extracellular matrix (ECM) proteins; the interaction between two such proteins, collagen and vitronectin (VN), is considered to be the key to controlling ECM remodeling in liver cirrhosis.
30984549	9	51	theme	rat	1478:1480	arg1	VN					1482:1483	rat VN	1478:1483	rat VN	1478:1483	The changes in rat VN due to CCl4 treatment did not correspond to the changes in plasma levels of human VN caused by LC, the ratio of active molecules, or carbohydrate composition, thereby indicating that CCl4-treated rats are not an appropriate model for studying VNs in human LC.
30984549	8	52	located	detected	1425:1432	arg1	carbohydrates					1437:1449	carbohydrates	1437:1449	carbohydrates of CCl4-VN	1437:1460	Increased fucosylation of LC-VN was not detected in carbohydrates of CCl4-VN.
30984549	8	52	located	detected	1425:1432	arg2	fucosylation					1395:1406	Increased fucosylation	1385:1406	Increased fucosylation of LC-VN	1385:1415	Increased fucosylation of LC-VN was not detected in carbohydrates of CCl4-VN.
30984549	8	53	theme	CCl4-VN	1454:1460	arg1	carbohydrates					1437:1449	carbohydrates	1437:1449	carbohydrates of CCl4-VN	1437:1460	Increased fucosylation of LC-VN was not detected in carbohydrates of CCl4-VN.
30984549	3	54	from	changes	609:615	arg1	activity					641:648	activity	641:648	activity	641:648	In this study, we examined the relationship between changes in VN glycosylation and activity related to the remodeling of hepatic tissue in human LC and a rat model of LC generated using carbon tetrachloride (CCl4).
30984549	3	54	from	changes	609:615	arg1	glycosylation					623:635	VN glycosylation	620:635	VN glycosylation	620:635	In this study, we examined the relationship between changes in VN glycosylation and activity related to the remodeling of hepatic tissue in human LC and a rat model of LC generated using carbon tetrachloride (CCl4).
30984549	2	55	theme	liver	540:544	arg1	cirrhosis					546:554	liver cirrhosis	540:554	liver cirrhosis	540:554	If it is possible to control the modification of oligosaccharides on VN, it may be possible to retard progression of liver cirrhosis.
30984549	6	56	theme	LC-VN	1146:1150	arg1	reactivities					1104:1115	Lectin reactivities	1097:1115	Lectin reactivities	1097:1115	Lectin reactivities and carbohydrate analyses of LC-VN revealed that branching, fucosylation, and sialylation of N-glycans were higher than those of normal VN.
30984549	6	56	theme	LC-VN	1146:1150	arg1	analyses					1134:1141	carbohydrate analyses	1121:1141	carbohydrate analyses of LC-VN	1121:1150	Lectin reactivities and carbohydrate analyses of LC-VN revealed that branching, fucosylation, and sialylation of N-glycans were higher than those of normal VN.
30984549	7	57	theme	CCl4-VN	1300:1306	arg1	level					1287:1291	the plasma level	1276:1291	the plasma level of rat CCl4-VN	1276:1306	On the other hand, the plasma level of rat CCl4-VN increased and the ratio of active molecules to collagen in plasma decreased.
30984549	9	58	theme	CCl4	1492:1495	arg1	treatment					1497:1505	CCl4 treatment	1492:1505	CCl4 treatment	1492:1505	The changes in rat VN due to CCl4 treatment did not correspond to the changes in plasma levels of human VN caused by LC, the ratio of active molecules, or carbohydrate composition, thereby indicating that CCl4-treated rats are not an appropriate model for studying VNs in human LC.
30984549	6	59	gly	fucosylation	1177:1188	arg1	N-glycans					1210:1218	N-glycans	1210:1218	N-glycans	1210:1218	Lectin reactivities and carbohydrate analyses of LC-VN revealed that branching, fucosylation, and sialylation of N-glycans were higher than those of normal VN.
30984549	10	60	theme	VNs	1770:1772	arg1	treatment					1757:1765	Glycosidase treatment	1745:1765	Glycosidase treatment of VNs	1745:1772	Glycosidase treatment of VNs supported the hypothesis that the collagen-binding activity of VN is modulated by alterations of glycosylation during LC, which may contribute to (a) the matrix incorporation of VN and (b) tissue fibrosis.
30984549	5	61	theme	binding	1020:1026	arg1	activities					1028:1037	similar binding activities	1012:1037	similar binding activities toward type I, IV, and V collagens	1012:1072	In contrast, purified LC-VN exhibited similar binding activities toward type I, IV, and V collagens to those of normal VN.
30984549	4	62	contain	has	907:909	arg2	activities					928:937	collagen-binding activities	911:937	collagen-binding activities	911:937	Plasma concentrations of VN in human LC declined to approximately two-thirds that in normal plasma, but the ratio of active VN, which has collagen-binding activities, increased 2.8 times in LC plasma.
30984549	4	62	contain	has	907:909	arg1	ratio					881:885	the ratio	877:885	the ratio	877:885	Plasma concentrations of VN in human LC declined to approximately two-thirds that in normal plasma, but the ratio of active VN, which has collagen-binding activities, increased 2.8 times in LC plasma.
30984549	6	63	theme	N-glycans	1210:1218	arg1	fucosylation					1177:1188	fucosylation	1177:1188	fucosylation	1177:1188	Lectin reactivities and carbohydrate analyses of LC-VN revealed that branching, fucosylation, and sialylation of N-glycans were higher than those of normal VN.
30984549	6	63	theme	N-glycans	1210:1218	arg1	sialylation					1195:1205	sialylation	1195:1205	sialylation	1195:1205	Lectin reactivities and carbohydrate analyses of LC-VN revealed that branching, fucosylation, and sialylation of N-glycans were higher than those of normal VN.
30984549	6	63	theme	N-glycans	1210:1218	arg1	branching					1166:1174	branching	1166:1174	branching	1166:1174	Lectin reactivities and carbohydrate analyses of LC-VN revealed that branching, fucosylation, and sialylation of N-glycans were higher than those of normal VN.
30984549	10	64	theme	Glycosidase	1745:1755	arg1	treatment					1757:1765	Glycosidase treatment	1745:1765	Glycosidase treatment of VNs	1745:1772	Glycosidase treatment of VNs supported the hypothesis that the collagen-binding activity of VN is modulated by alterations of glycosylation during LC, which may contribute to (a) the matrix incorporation of VN and (b) tissue fibrosis.
30984549	4	65	from	two-thirds	839:848	arg1	plasma					865:870	normal plasma	858:870	normal plasma	858:870	Plasma concentrations of VN in human LC declined to approximately two-thirds that in normal plasma, but the ratio of active VN, which has collagen-binding activities, increased 2.8 times in LC plasma.
30984549	10	66	theme	VN	1952:1953	arg1	fibrosis					1970:1977	(b) tissue fibrosis	1959:1977	(b) tissue fibrosis	1959:1977	Glycosidase treatment of VNs supported the hypothesis that the collagen-binding activity of VN is modulated by alterations of glycosylation during LC, which may contribute to (a) the matrix incorporation of VN and (b) tissue fibrosis.
30984549	10	66	theme	VN	1952:1953	arg1	incorporation					1935:1947	the matrix incorporation	1924:1947	(a) the matrix incorporation of VN	1920:1953	Glycosidase treatment of VNs supported the hypothesis that the collagen-binding activity of VN is modulated by alterations of glycosylation during LC, which may contribute to (a) the matrix incorporation of VN and (b) tissue fibrosis.
30984549	9	67	theme	human	1561:1565	arg1	VN					1567:1568	human VN	1561:1568	human VN	1561:1568	The changes in rat VN due to CCl4 treatment did not correspond to the changes in plasma levels of human VN caused by LC, the ratio of active molecules, or carbohydrate composition, thereby indicating that CCl4-treated rats are not an appropriate model for studying VNs in human LC.
30984549	9	68	from	VNs	1728:1730	arg1	LC					1741:1742	human LC	1735:1742	human LC	1735:1742	The changes in rat VN due to CCl4 treatment did not correspond to the changes in plasma levels of human VN caused by LC, the ratio of active molecules, or carbohydrate composition, thereby indicating that CCl4-treated rats are not an appropriate model for studying VNs in human LC.
30984549	10	69	theme	matrix	1928:1933	arg1	incorporation					1935:1947	the matrix incorporation	1924:1947	(a) the matrix incorporation of VN	1920:1953	Glycosidase treatment of VNs supported the hypothesis that the collagen-binding activity of VN is modulated by alterations of glycosylation during LC, which may contribute to (a) the matrix incorporation of VN and (b) tissue fibrosis.
30984549	3	70	theme	LC	725:726	arg1	changes					609:615	changes	609:615	changes in VN glycosylation and activity related to the remodeling of hepatic tissue in human LC	609:704	In this study, we examined the relationship between changes in VN glycosylation and activity related to the remodeling of hepatic tissue in human LC and a rat model of LC generated using carbon tetrachloride (CCl4).
30984549	3	70	theme	LC	725:726	arg1	model					716:720	a rat model	710:720	a rat model of LC generated using carbon tetrachloride (CCl4)	710:770	In this study, we examined the relationship between changes in VN glycosylation and activity related to the remodeling of hepatic tissue in human LC and a rat model of LC generated using carbon tetrachloride (CCl4).
30984549	1	71	theme	ECM	388:390	arg1	remodeling					392:401	ECM remodeling	388:401	ECM remodeling in liver cirrhosis	388:420	Liver cirrhosis (LC) is a disease characterized by pathological accumulation and alteration of extracellular matrix (ECM) proteins; the interaction between two such proteins, collagen and vitronectin (VN), is considered to be the key to controlling ECM remodeling in liver cirrhosis.
30984549	10	72	theme	glycosylation	1871:1883	arg1	alterations					1856:1866	alterations	1856:1866	alterations of glycosylation	1856:1883	Glycosidase treatment of VNs supported the hypothesis that the collagen-binding activity of VN is modulated by alterations of glycosylation during LC, which may contribute to (a) the matrix incorporation of VN and (b) tissue fibrosis.
30984549	0	73	theme	glycosylation	85:97	arg1	pattern					99:105	their glycosylation pattern	79:105	their glycosylation pattern	79:105	Vitronectins produced by human cirrhotic liver and CCl4-treated rats differ in their glycosylation pattern and tissue remodeling activity.
30984549	6	74	theme	normal	1246:1251	arg1	VN					1253:1254	normal VN	1246:1254	normal VN	1246:1254	Lectin reactivities and carbohydrate analyses of LC-VN revealed that branching, fucosylation, and sialylation of N-glycans were higher than those of normal VN.
30984549	5	75	theme	similar	1012:1018	arg1	activities					1028:1037	similar binding activities	1012:1037	similar binding activities toward type I, IV, and V collagens	1012:1072	In contrast, purified LC-VN exhibited similar binding activities toward type I, IV, and V collagens to those of normal VN.
30984549	9	76	from	changes	1533:1539	arg1	levels					1551:1556	plasma levels	1544:1556	plasma levels of human VN	1544:1568	The changes in rat VN due to CCl4 treatment did not correspond to the changes in plasma levels of human VN caused by LC, the ratio of active molecules, or carbohydrate composition, thereby indicating that CCl4-treated rats are not an appropriate model for studying VNs in human LC.
30984549	2	77	theme	oligosaccharides	472:487	arg1	modification					456:467	the modification	452:467	the modification of oligosaccharides on VN	452:493	If it is possible to control the modification of oligosaccharides on VN, it may be possible to retard progression of liver cirrhosis.
30984549	10	78	theme	VN	1837:1838	arg1	activity					1825:1832	the collagen-binding activity	1804:1832	the collagen-binding activity of VN	1804:1838	Glycosidase treatment of VNs supported the hypothesis that the collagen-binding activity of VN is modulated by alterations of glycosylation during LC, which may contribute to (a) the matrix incorporation of VN and (b) tissue fibrosis.
30984549	0	79	theme	tissue	111:116	arg1	activity					129:136	tissue remodeling activity	111:136	tissue remodeling activity	111:136	Vitronectins produced by human cirrhotic liver and CCl4-treated rats differ in their glycosylation pattern and tissue remodeling activity.
30984549	3	80	theme	hepatic	679:685	arg1	tissue					687:692	hepatic tissue	679:692	hepatic tissue	679:692	In this study, we examined the relationship between changes in VN glycosylation and activity related to the remodeling of hepatic tissue in human LC and a rat model of LC generated using carbon tetrachloride (CCl4).
30984549	9	81	from	changes	1467:1473	arg1	VN					1482:1483	rat VN	1478:1483	rat VN	1478:1483	The changes in rat VN due to CCl4 treatment did not correspond to the changes in plasma levels of human VN caused by LC, the ratio of active molecules, or carbohydrate composition, thereby indicating that CCl4-treated rats are not an appropriate model for studying VNs in human LC.
30984549	9	82	theme	molecules	1604:1612	arg1	changes					1533:1539	the changes	1529:1539	the changes in plasma levels of human VN caused by LC	1529:1581	The changes in rat VN due to CCl4 treatment did not correspond to the changes in plasma levels of human VN caused by LC, the ratio of active molecules, or carbohydrate composition, thereby indicating that CCl4-treated rats are not an appropriate model for studying VNs in human LC.
30984549	9	82	theme	molecules	1604:1612	arg1	composition					1631:1641	carbohydrate composition	1618:1641	carbohydrate composition	1618:1641	The changes in rat VN due to CCl4 treatment did not correspond to the changes in plasma levels of human VN caused by LC, the ratio of active molecules, or carbohydrate composition, thereby indicating that CCl4-treated rats are not an appropriate model for studying VNs in human LC.
30984549	9	82	theme	molecules	1604:1612	arg1	ratio					1588:1592	the ratio	1584:1592	the ratio of active molecules	1584:1612	The changes in rat VN due to CCl4 treatment did not correspond to the changes in plasma levels of human VN caused by LC, the ratio of active molecules, or carbohydrate composition, thereby indicating that CCl4-treated rats are not an appropriate model for studying VNs in human LC.
30984549	0	83	theme	human	25:29	arg1	liver					41:45	human cirrhotic liver	25:45	human cirrhotic liver	25:45	Vitronectins produced by human cirrhotic liver and CCl4-treated rats differ in their glycosylation pattern and tissue remodeling activity.
30984549	8	84	theme	LC-VN	1411:1415	arg1	fucosylation					1395:1406	Increased fucosylation	1385:1406	Increased fucosylation of LC-VN	1385:1415	Increased fucosylation of LC-VN was not detected in carbohydrates of CCl4-VN.
30984549	5	85	theme	V	1062:1062	arg1	collagens					1064:1072	V collagens	1062:1072	V collagens	1062:1072	In contrast, purified LC-VN exhibited similar binding activities toward type I, IV, and V collagens to those of normal VN.
30984549	4	86	theme	active	890:895	arg1	VN					897:898	active VN	890:898	active VN	890:898	Plasma concentrations of VN in human LC declined to approximately two-thirds that in normal plasma, but the ratio of active VN, which has collagen-binding activities, increased 2.8 times in LC plasma.
30984549	0	87	theme	CCl4-treated	51:62	arg1	rats					64:67	CCl4-treated rats	51:67	CCl4-treated rats	51:67	Vitronectins produced by human cirrhotic liver and CCl4-treated rats differ in their glycosylation pattern and tissue remodeling activity.
30984549	1	88	theme	such	299:302	arg1	vitronectin					327:337	vitronectin	327:337	vitronectin (VN)	327:342	Liver cirrhosis (LC) is a disease characterized by pathological accumulation and alteration of extracellular matrix (ECM) proteins; the interaction between two such proteins, collagen and vitronectin (VN), is considered to be the key to controlling ECM remodeling in liver cirrhosis.
30984549	1	88	theme	such	299:302	arg1	collagen					314:321	collagen	314:321	collagen	314:321	Liver cirrhosis (LC) is a disease characterized by pathological accumulation and alteration of extracellular matrix (ECM) proteins; the interaction between two such proteins, collagen and vitronectin (VN), is considered to be the key to controlling ECM remodeling in liver cirrhosis.
30984549	1	88	theme	such	299:302	arg1	proteins					304:311	two such proteins	295:311	two such proteins	295:311	Liver cirrhosis (LC) is a disease characterized by pathological accumulation and alteration of extracellular matrix (ECM) proteins; the interaction between two such proteins, collagen and vitronectin (VN), is considered to be the key to controlling ECM remodeling in liver cirrhosis.
30984549	3	89	from	model	716:720	arg1	activity					641:648	activity	641:648	activity	641:648	In this study, we examined the relationship between changes in VN glycosylation and activity related to the remodeling of hepatic tissue in human LC and a rat model of LC generated using carbon tetrachloride (CCl4).
30984549	3	89	from	model	716:720	arg1	glycosylation					623:635	VN glycosylation	620:635	VN glycosylation	620:635	In this study, we examined the relationship between changes in VN glycosylation and activity related to the remodeling of hepatic tissue in human LC and a rat model of LC generated using carbon tetrachloride (CCl4).
30984549	4	90	theme	LC	963:964	arg1	plasma					966:971	LC plasma	963:971	LC plasma	963:971	Plasma concentrations of VN in human LC declined to approximately two-thirds that in normal plasma, but the ratio of active VN, which has collagen-binding activities, increased 2.8 times in LC plasma.
30984549	7	91	theme	molecules	1342:1350	arg1	ratio					1326:1330	the ratio	1322:1330	the ratio of active molecules to collagen in plasma	1322:1372	On the other hand, the plasma level of rat CCl4-VN increased and the ratio of active molecules to collagen in plasma decreased.
30984549	9	92	theme	active	1597:1602	arg1	molecules					1604:1612	active molecules	1597:1612	active molecules	1597:1612	The changes in rat VN due to CCl4 treatment did not correspond to the changes in plasma levels of human VN caused by LC, the ratio of active molecules, or carbohydrate composition, thereby indicating that CCl4-treated rats are not an appropriate model for studying VNs in human LC.
30984549	1	93	theme	matrix	248:253	arg1	proteins					261:268	extracellular matrix (ECM) proteins	234:268	extracellular matrix (ECM) proteins	234:268	Liver cirrhosis (LC) is a disease characterized by pathological accumulation and alteration of extracellular matrix (ECM) proteins; the interaction between two such proteins, collagen and vitronectin (VN), is considered to be the key to controlling ECM remodeling in liver cirrhosis.
31008057	8	0	theme	precursor	1310:1318	arg1	synthesis					1320:1328	glycan precursor synthesis	1303:1328	glycan precursor synthesis	1303:1328	Simulations suggested that these growth-coupled glycan overproducing strains had metabolic imbalances that rerouted flux toward glycan precursor synthesis.
31008057	4	1	theme	glycoprotein	650:661	arg1	production					663:672	prokaryotic glycoprotein production	638:672	prokaryotic glycoprotein production	638:672	However, prokaryotic glycoprotein production has several drawbacks, including insufficient availability of non-native glycan precursors.
31008057	9	2	theme	model-identified	1353:1368	arg1	strains					1379:1385	the model-identified knockout strains	1349:1385	the model-identified knockout strains	1349:1385	We then validated the model-identified knockout strains experimentally by measuring glycan expression using a flow cytometric-based assay involving fluorescent labeling of cell surface-displayed glycans.
31008057	6	3	theme	C. jejuni	1010:1018	arg1	assembly					1027:1034	C. jejuni glycan assembly	1010:1034	C. jejuni glycan assembly	1010:1034	First, we incorporated reactions associated with C. jejuni glycan assembly into a genome-scale model of E. coli metabolism.
31008057	4	4	theme	precursors	754:763	arg1	availability					720:731	insufficient availability	707:731	insufficient availability of non-native glycan precursors	707:763	However, prokaryotic glycoprotein production has several drawbacks, including insufficient availability of non-native glycan precursors.
31008057	3	5	theme	glycoprotein	589:600	arg1	production					602:611	glycoprotein production	589:611	glycoprotein production in prokaryotes	589:626	The discovery of a protein glycosylation pathway in the pathogen Campylobacter jejuni and its subsequent transfer into laboratory strains of Escherichia coli has spurred great interest in glycoprotein production in prokaryotes.
31008057	2	6	theme	proteins	344:351	arg1	immunogenicity					309:322	immunogenicity	309:322	immunogenicity	309:322	Glycosylation is also critical to the pharmacokinetic activity and immunogenicity of many therapeutic proteins currently produced in complex eukaryotic hosts.
31008057	2	6	theme	proteins	344:351	arg1	activity					296:303	pharmacokinetic activity	280:303	pharmacokinetic activity	280:303	Glycosylation is also critical to the pharmacokinetic activity and immunogenicity of many therapeutic proteins currently produced in complex eukaryotic hosts.
31008057	9	7	theme	glycan	1415:1420	arg1	expression					1422:1431	glycan expression	1415:1431	glycan expression	1415:1431	We then validated the model-identified knockout strains experimentally by measuring glycan expression using a flow cytometric-based assay involving fluorescent labeling of cell surface-displayed glycans.
31008057	2	8	theme	many	327:330	arg1	proteins					344:351	many therapeutic proteins	327:351	many therapeutic proteins currently produced in complex eukaryotic hosts	327:398	Glycosylation is also critical to the pharmacokinetic activity and immunogenicity of many therapeutic proteins currently produced in complex eukaryotic hosts.
31008057	4	9	theme	non-native	736:745	arg1	precursors					754:763	non-native glycan precursors	736:763	non-native glycan precursors	736:763	However, prokaryotic glycoprotein production has several drawbacks, including insufficient availability of non-native glycan precursors.
31008057	5	10	theme	constraint-based	804:819	arg1	model					821:825	a constraint-based model	802:825	a constraint-based model of E. coli metabolism	802:847	To address this limitation, we used a constraint-based model of E. coli metabolism in combination with heuristic optimization to design gene knockout strains that overproduced glycan precursors.
31008057	4	11	contain	has	674:676	arg1	production					663:672	prokaryotic glycoprotein production	638:672	prokaryotic glycoprotein production	638:672	However, prokaryotic glycoprotein production has several drawbacks, including insufficient availability of non-native glycan precursors.
31008057	4	11	contain	has	674:676	arg2	drawbacks					686:694	several drawbacks	678:694	several drawbacks	678:694	However, prokaryotic glycoprotein production has several drawbacks, including insufficient availability of non-native glycan precursors.
31008057	4	11	contain	has	674:676	arg2	availability					720:731	insufficient availability	707:731	insufficient availability of non-native glycan precursors	707:763	However, prokaryotic glycoprotein production has several drawbacks, including insufficient availability of non-native glycan precursors.
31008057	1	12	theme	proteome	232:239	arg1	two-thirds					214:223	two-thirds	214:223	two-thirds	214:223	Asparagine-linked (N-linked) glycosylation is the most common protein modification in eukaryotes, affecting over two-thirds of the proteome.
31008057	1	12	theme	proteome	232:239	arg1	proteome					232:239	the proteome	228:239	the proteome	228:239	Asparagine-linked (N-linked) glycosylation is the most common protein modification in eukaryotes, affecting over two-thirds of the proteome.
31008057	5	13	used	used	797:800	arg2	we					794:795	we	794:795	we	794:795	To address this limitation, we used a constraint-based model of E. coli metabolism in combination with heuristic optimization to design gene knockout strains that overproduced glycan precursors.
31008057	6	14	theme	E. coli	1065:1071	arg1	metabolism					1073:1082	E. coli metabolism	1065:1082	E. coli metabolism	1065:1082	First, we incorporated reactions associated with C. jejuni glycan assembly into a genome-scale model of E. coli metabolism.
31008057	9	15	theme	flow	1441:1444	arg1	assay					1463:1467	a flow cytometric-based assay	1439:1467	a flow cytometric-based assay involving fluorescent labeling of cell surface-displayed glycans	1439:1532	We then validated the model-identified knockout strains experimentally by measuring glycan expression using a flow cytometric-based assay involving fluorescent labeling of cell surface-displayed glycans.
31008057	0	16	theme	model-guided	65:76	arg1	engineering					88:98	model-guided metabolic engineering	65:98	model-guided metabolic engineering	65:98	Improving designer glycan production in Escherichia coli through model-guided metabolic engineering.
31008057	9	17	theme	fluorescent	1479:1489	arg1	labeling					1491:1498	fluorescent labeling	1479:1498	fluorescent labeling of cell surface-displayed glycans	1479:1532	We then validated the model-identified knockout strains experimentally by measuring glycan expression using a flow cytometric-based assay involving fluorescent labeling of cell surface-displayed glycans.
31008057	5	18	theme	overproduced	929:940	arg1	precursors					949:958	overproduced glycan precursors	929:958	overproduced glycan precursors	929:958	To address this limitation, we used a constraint-based model of E. coli metabolism in combination with heuristic optimization to design gene knockout strains that overproduced glycan precursors.
31008057	7	19	theme	gene	1104:1107	arg1	strains					1118:1124	gene knockout strains	1104:1124	gene knockout strains that coupled optimal growth to glycan synthesis	1104:1172	We then identified gene knockout strains that coupled optimal growth to glycan synthesis.
31008057	1	20	theme	protein	163:169	arg1	glycosylation					130:142	Asparagine-linked (N-linked) glycosylation	101:142	Asparagine-linked (N-linked) glycosylation	101:142	Asparagine-linked (N-linked) glycosylation is the most common protein modification in eukaryotes, affecting over two-thirds of the proteome.
31008057	1	20	theme	protein	163:169	arg1	modification					171:182	the most common protein modification	147:182	the most common protein modification in eukaryotes	147:196	Asparagine-linked (N-linked) glycosylation is the most common protein modification in eukaryotes, affecting over two-thirds of the proteome.
31008057	10	21	theme	microbial	1671:1679	arg1	platform					1695:1702	a next-generation microbial glycosylation platform	1653:1702	a next-generation microbial glycosylation platform	1653:1702	Overall, this study demonstrates the promising role that metabolic modeling can play in optimizing the performance of a next-generation microbial glycosylation platform.
31008057	3	22	gly	glycoprotein	589:600	arg1	glycoprotein					589:600	glycoprotein production	589:611	glycoprotein production in prokaryotes	589:626	The discovery of a protein glycosylation pathway in the pathogen Campylobacter jejuni and its subsequent transfer into laboratory strains of Escherichia coli has spurred great interest in glycoprotein production in prokaryotes.
31008057	5	23	theme	heuristic	869:877	arg1	optimization					879:890	heuristic optimization	869:890	heuristic optimization to design gene knockout strains that overproduced glycan precursors	869:958	To address this limitation, we used a constraint-based model of E. coli metabolism in combination with heuristic optimization to design gene knockout strains that overproduced glycan precursors.
31008057	1	24	from	modification	171:182	arg1	eukaryotes					187:196	eukaryotes	187:196	eukaryotes	187:196	Asparagine-linked (N-linked) glycosylation is the most common protein modification in eukaryotes, affecting over two-thirds of the proteome.
31008057	10	25	theme	metabolic	1592:1600	arg1	modeling					1602:1609	metabolic modeling	1592:1609	metabolic modeling	1592:1609	Overall, this study demonstrates the promising role that metabolic modeling can play in optimizing the performance of a next-generation microbial glycosylation platform.
31008057	9	26	theme	surface-displayed	1508:1524	arg1	glycans					1526:1532	cell surface-displayed glycans	1503:1532	cell surface-displayed glycans	1503:1532	We then validated the model-identified knockout strains experimentally by measuring glycan expression using a flow cytometric-based assay involving fluorescent labeling of cell surface-displayed glycans.
31008057	5	27	theme	metabolism	838:847	arg1	model					821:825	a constraint-based model	802:825	a constraint-based model of E. coli metabolism	802:847	To address this limitation, we used a constraint-based model of E. coli metabolism in combination with heuristic optimization to design gene knockout strains that overproduced glycan precursors.
31008057	3	28	theme	protein	420:426	arg1	pathway					442:448	a protein glycosylation pathway	418:448	a protein glycosylation pathway in the pathogen Campylobacter jejuni	418:485	The discovery of a protein glycosylation pathway in the pathogen Campylobacter jejuni and its subsequent transfer into laboratory strains of Escherichia coli has spurred great interest in glycoprotein production in prokaryotes.
31008057	10	29	theme	platform	1695:1702	arg1	performance					1638:1648	the performance	1634:1648	the performance of a next-generation microbial glycosylation platform	1634:1702	Overall, this study demonstrates the promising role that metabolic modeling can play in optimizing the performance of a next-generation microbial glycosylation platform.
31008057	3	30	theme	Campylobacter	466:478	arg1	jejuni					480:485	the pathogen Campylobacter jejuni	453:485	the pathogen Campylobacter jejuni	453:485	The discovery of a protein glycosylation pathway in the pathogen Campylobacter jejuni and its subsequent transfer into laboratory strains of Escherichia coli has spurred great interest in glycoprotein production in prokaryotes.
31008057	9	31	theme	knockout	1370:1377	arg1	strains					1379:1385	the model-identified knockout strains	1349:1385	the model-identified knockout strains	1349:1385	We then validated the model-identified knockout strains experimentally by measuring glycan expression using a flow cytometric-based assay involving fluorescent labeling of cell surface-displayed glycans.
31008057	0	32	theme	glycan	19:24	arg1	production					26:35	designer glycan production	10:35	designer glycan production in Escherichia coli	10:55	Improving designer glycan production in Escherichia coli through model-guided metabolic engineering.
31008057	8	33	theme	glycan	1223:1228	arg1	strains					1244:1250	these growth-coupled glycan overproducing strains	1202:1250	these growth-coupled glycan overproducing strains	1202:1250	Simulations suggested that these growth-coupled glycan overproducing strains had metabolic imbalances that rerouted flux toward glycan precursor synthesis.
31008057	3	34	theme	subsequent	495:504	arg1	transfer					506:513	its subsequent transfer	491:513	its subsequent transfer into laboratory strains of Escherichia coli	491:557	The discovery of a protein glycosylation pathway in the pathogen Campylobacter jejuni and its subsequent transfer into laboratory strains of Escherichia coli has spurred great interest in glycoprotein production in prokaryotes.
31008057	3	35	theme	pathway	442:448	arg1	discovery					405:413	The discovery	401:413	The discovery of a protein glycosylation pathway in the pathogen Campylobacter jejuni and its subsequent transfer into laboratory strains of Escherichia coli	401:557	The discovery of a protein glycosylation pathway in the pathogen Campylobacter jejuni and its subsequent transfer into laboratory strains of Escherichia coli has spurred great interest in glycoprotein production in prokaryotes.
31008057	3	36	from	transfer	506:513	arg1	jejuni					480:485	the pathogen Campylobacter jejuni	453:485	the pathogen Campylobacter jejuni	453:485	The discovery of a protein glycosylation pathway in the pathogen Campylobacter jejuni and its subsequent transfer into laboratory strains of Escherichia coli has spurred great interest in glycoprotein production in prokaryotes.
31008057	2	37	theme	eukaryotic	383:392	arg1	hosts					394:398	complex eukaryotic hosts	375:398	complex eukaryotic hosts	375:398	Glycosylation is also critical to the pharmacokinetic activity and immunogenicity of many therapeutic proteins currently produced in complex eukaryotic hosts.
31008057	5	38	theme	gene	902:905	arg1	strains					916:922	design gene knockout strains	895:922	design gene knockout strains that overproduced glycan precursors	895:958	To address this limitation, we used a constraint-based model of E. coli metabolism in combination with heuristic optimization to design gene knockout strains that overproduced glycan precursors.
31008057	8	39	theme	metabolic	1256:1264	arg1	imbalances					1266:1275	metabolic imbalances	1256:1275	metabolic imbalances that rerouted flux toward glycan precursor synthesis	1256:1328	Simulations suggested that these growth-coupled glycan overproducing strains had metabolic imbalances that rerouted flux toward glycan precursor synthesis.
31008057	4	40	theme	several	678:684	arg1	drawbacks					686:694	several drawbacks	678:694	several drawbacks	678:694	However, prokaryotic glycoprotein production has several drawbacks, including insufficient availability of non-native glycan precursors.
31008057	4	40	theme	several	678:684	arg1	availability					720:731	insufficient availability	707:731	insufficient availability of non-native glycan precursors	707:763	However, prokaryotic glycoprotein production has several drawbacks, including insufficient availability of non-native glycan precursors.
31008057	1	41	link	N-linked	120:127	arg1	glycosylation					130:142	Asparagine-linked (N-linked) glycosylation	101:142	Asparagine-linked (N-linked) glycosylation	101:142	Asparagine-linked (N-linked) glycosylation is the most common protein modification in eukaryotes, affecting over two-thirds of the proteome.
31008057	1	41	link	N-linked	120:127	arg1	modification					171:182	the most common protein modification	147:182	the most common protein modification in eukaryotes	147:196	Asparagine-linked (N-linked) glycosylation is the most common protein modification in eukaryotes, affecting over two-thirds of the proteome.
31008057	3	42	from	jejuni	480:485	arg1	discovery					405:413	The discovery	401:413	The discovery of a protein glycosylation pathway in the pathogen Campylobacter jejuni and its subsequent transfer into laboratory strains of Escherichia coli	401:557	The discovery of a protein glycosylation pathway in the pathogen Campylobacter jejuni and its subsequent transfer into laboratory strains of Escherichia coli has spurred great interest in glycoprotein production in prokaryotes.
31008057	3	43	theme	great	571:575	arg1	interest					577:584	great interest	571:584	great interest in glycoprotein production in prokaryotes	571:626	The discovery of a protein glycosylation pathway in the pathogen Campylobacter jejuni and its subsequent transfer into laboratory strains of Escherichia coli has spurred great interest in glycoprotein production in prokaryotes.
31008057	6	44	theme	genome-scale	1043:1054	arg1	model					1056:1060	a genome-scale model	1041:1060	a genome-scale model of E. coli metabolism	1041:1082	First, we incorporated reactions associated with C. jejuni glycan assembly into a genome-scale model of E. coli metabolism.
31008057	1	45	link	Asparagine-linked	101:117	arg1	glycosylation					130:142	Asparagine-linked (N-linked) glycosylation	101:142	Asparagine-linked (N-linked) glycosylation	101:142	Asparagine-linked (N-linked) glycosylation is the most common protein modification in eukaryotes, affecting over two-thirds of the proteome.
31008057	1	45	link	Asparagine-linked	101:117	arg1	modification					171:182	the most common protein modification	147:182	the most common protein modification in eukaryotes	147:196	Asparagine-linked (N-linked) glycosylation is the most common protein modification in eukaryotes, affecting over two-thirds of the proteome.
31008057	8	46	theme	glycan	1303:1308	arg1	synthesis					1320:1328	glycan precursor synthesis	1303:1328	glycan precursor synthesis	1303:1328	Simulations suggested that these growth-coupled glycan overproducing strains had metabolic imbalances that rerouted flux toward glycan precursor synthesis.
31008057	4	47	theme	prokaryotic	638:648	arg1	production					663:672	prokaryotic glycoprotein production	638:672	prokaryotic glycoprotein production	638:672	However, prokaryotic glycoprotein production has several drawbacks, including insufficient availability of non-native glycan precursors.
31008057	6	48	theme	glycan	1020:1025	arg1	assembly					1027:1034	C. jejuni glycan assembly	1010:1034	C. jejuni glycan assembly	1010:1034	First, we incorporated reactions associated with C. jejuni glycan assembly into a genome-scale model of E. coli metabolism.
31008057	3	49	from	interest	577:584	arg1	production					602:611	glycoprotein production	589:611	glycoprotein production in prokaryotes	589:626	The discovery of a protein glycosylation pathway in the pathogen Campylobacter jejuni and its subsequent transfer into laboratory strains of Escherichia coli has spurred great interest in glycoprotein production in prokaryotes.
31008057	4	50	theme	glycan	747:752	arg1	precursors					754:763	non-native glycan precursors	736:763	non-native glycan precursors	736:763	However, prokaryotic glycoprotein production has several drawbacks, including insufficient availability of non-native glycan precursors.
31008057	3	51	from	pathway	442:448	arg1	jejuni					480:485	the pathogen Campylobacter jejuni	453:485	the pathogen Campylobacter jejuni	453:485	The discovery of a protein glycosylation pathway in the pathogen Campylobacter jejuni and its subsequent transfer into laboratory strains of Escherichia coli has spurred great interest in glycoprotein production in prokaryotes.
31008057	2	52	theme	therapeutic	332:342	arg1	proteins					344:351	many therapeutic proteins	327:351	many therapeutic proteins currently produced in complex eukaryotic hosts	327:398	Glycosylation is also critical to the pharmacokinetic activity and immunogenicity of many therapeutic proteins currently produced in complex eukaryotic hosts.
31008057	5	53	theme	glycan	942:947	arg1	precursors					949:958	overproduced glycan precursors	929:958	overproduced glycan precursors	929:958	To address this limitation, we used a constraint-based model of E. coli metabolism in combination with heuristic optimization to design gene knockout strains that overproduced glycan precursors.
31008057	4	54	theme	insufficient	707:718	arg1	availability					720:731	insufficient availability	707:731	insufficient availability of non-native glycan precursors	707:763	However, prokaryotic glycoprotein production has several drawbacks, including insufficient availability of non-native glycan precursors.
31008057	2	55	theme	complex	375:381	arg1	hosts					394:398	complex eukaryotic hosts	375:398	complex eukaryotic hosts	375:398	Glycosylation is also critical to the pharmacokinetic activity and immunogenicity of many therapeutic proteins currently produced in complex eukaryotic hosts.
31008057	6	56	theme	metabolism	1073:1082	arg1	model					1056:1060	a genome-scale model	1041:1060	a genome-scale model of E. coli metabolism	1041:1082	First, we incorporated reactions associated with C. jejuni glycan assembly into a genome-scale model of E. coli metabolism.
31008057	3	57	from	discovery	405:413	arg1	jejuni					480:485	the pathogen Campylobacter jejuni	453:485	the pathogen Campylobacter jejuni	453:485	The discovery of a protein glycosylation pathway in the pathogen Campylobacter jejuni and its subsequent transfer into laboratory strains of Escherichia coli has spurred great interest in glycoprotein production in prokaryotes.
31008057	7	58	theme	glycan	1157:1162	arg1	synthesis					1164:1172	glycan synthesis	1157:1172	glycan synthesis	1157:1172	We then identified gene knockout strains that coupled optimal growth to glycan synthesis.
31008057	9	59	theme	cytometric-based	1446:1461	arg1	assay					1463:1467	a flow cytometric-based assay	1439:1467	a flow cytometric-based assay involving fluorescent labeling of cell surface-displayed glycans	1439:1532	We then validated the model-identified knockout strains experimentally by measuring glycan expression using a flow cytometric-based assay involving fluorescent labeling of cell surface-displayed glycans.
31008057	2	60	theme	pharmacokinetic	280:294	arg1	activity					296:303	pharmacokinetic activity	280:303	pharmacokinetic activity	280:303	Glycosylation is also critical to the pharmacokinetic activity and immunogenicity of many therapeutic proteins currently produced in complex eukaryotic hosts.
31008057	7	61	theme	optimal	1139:1145	arg1	growth					1147:1152	optimal growth	1139:1152	optimal growth	1139:1152	We then identified gene knockout strains that coupled optimal growth to glycan synthesis.
31008057	0	62	theme	metabolic	78:86	arg1	engineering					88:98	model-guided metabolic engineering	65:98	model-guided metabolic engineering	65:98	Improving designer glycan production in Escherichia coli through model-guided metabolic engineering.
31008057	1	63	theme	common	156:161	arg1	glycosylation					130:142	Asparagine-linked (N-linked) glycosylation	101:142	Asparagine-linked (N-linked) glycosylation	101:142	Asparagine-linked (N-linked) glycosylation is the most common protein modification in eukaryotes, affecting over two-thirds of the proteome.
31008057	1	63	theme	common	156:161	arg1	modification					171:182	the most common protein modification	147:182	the most common protein modification in eukaryotes	147:196	Asparagine-linked (N-linked) glycosylation is the most common protein modification in eukaryotes, affecting over two-thirds of the proteome.
31008057	0	64	from	production	26:35	arg1	coli					52:55	Escherichia coli	40:55	Escherichia coli	40:55	Improving designer glycan production in Escherichia coli through model-guided metabolic engineering.
31008057	5	65	with	combination	852:862	arg1	optimization					879:890	heuristic optimization	869:890	heuristic optimization to design gene knockout strains that overproduced glycan precursors	869:958	To address this limitation, we used a constraint-based model of E. coli metabolism in combination with heuristic optimization to design gene knockout strains that overproduced glycan precursors.
31008057	2	66	dep	activity	296:303	arg1	the					276:278	the	276:278	the	276:278	Glycosylation is also critical to the pharmacokinetic activity and immunogenicity of many therapeutic proteins currently produced in complex eukaryotic hosts.
31008057	7	67	theme	knockout	1109:1116	arg1	strains					1118:1124	gene knockout strains	1104:1124	gene knockout strains that coupled optimal growth to glycan synthesis	1104:1172	We then identified gene knockout strains that coupled optimal growth to glycan synthesis.
31008057	10	68	theme	next-generation	1655:1669	arg1	platform					1695:1702	a next-generation microbial glycosylation platform	1653:1702	a next-generation microbial glycosylation platform	1653:1702	Overall, this study demonstrates the promising role that metabolic modeling can play in optimizing the performance of a next-generation microbial glycosylation platform.
31008057	3	69	from	production	602:611	arg1	prokaryotes					616:626	prokaryotes	616:626	prokaryotes	616:626	The discovery of a protein glycosylation pathway in the pathogen Campylobacter jejuni and its subsequent transfer into laboratory strains of Escherichia coli has spurred great interest in glycoprotein production in prokaryotes.
31008057	9	70	theme	cell	1503:1506	arg1	glycans					1526:1532	cell surface-displayed glycans	1503:1532	cell surface-displayed glycans	1503:1532	We then validated the model-identified knockout strains experimentally by measuring glycan expression using a flow cytometric-based assay involving fluorescent labeling of cell surface-displayed glycans.
31008057	5	71	theme	E. coli	830:836	arg1	metabolism					838:847	E. coli metabolism	830:847	E. coli metabolism	830:847	To address this limitation, we used a constraint-based model of E. coli metabolism in combination with heuristic optimization to design gene knockout strains that overproduced glycan precursors.
31008057	9	72	theme	glycans	1526:1532	arg1	labeling					1491:1498	fluorescent labeling	1479:1498	fluorescent labeling of cell surface-displayed glycans	1479:1532	We then validated the model-identified knockout strains experimentally by measuring glycan expression using a flow cytometric-based assay involving fluorescent labeling of cell surface-displayed glycans.
31008057	5	73	theme	knockout	907:914	arg1	strains					916:922	design gene knockout strains	895:922	design gene knockout strains that overproduced glycan precursors	895:958	To address this limitation, we used a constraint-based model of E. coli metabolism in combination with heuristic optimization to design gene knockout strains that overproduced glycan precursors.
31008057	8	74	theme	growth-coupled	1208:1221	arg1	strains					1244:1250	these growth-coupled glycan overproducing strains	1202:1250	these growth-coupled glycan overproducing strains	1202:1250	Simulations suggested that these growth-coupled glycan overproducing strains had metabolic imbalances that rerouted flux toward glycan precursor synthesis.
31008057	4	75	gly	glycoprotein	650:661	arg1	glycoprotein					650:661	prokaryotic glycoprotein production	638:672	prokaryotic glycoprotein production	638:672	However, prokaryotic glycoprotein production has several drawbacks, including insufficient availability of non-native glycan precursors.
31008057	1	76	theme	Asparagine-linked	101:117	arg1	glycosylation					130:142	Asparagine-linked (N-linked) glycosylation	101:142	Asparagine-linked (N-linked) glycosylation	101:142	Asparagine-linked (N-linked) glycosylation is the most common protein modification in eukaryotes, affecting over two-thirds of the proteome.
31008057	1	76	theme	Asparagine-linked	101:117	arg1	modification					171:182	the most common protein modification	147:182	the most common protein modification in eukaryotes	147:196	Asparagine-linked (N-linked) glycosylation is the most common protein modification in eukaryotes, affecting over two-thirds of the proteome.
31008057	3	77	theme	glycosylation	428:440	arg1	pathway					442:448	a protein glycosylation pathway	418:448	a protein glycosylation pathway in the pathogen Campylobacter jejuni	418:485	The discovery of a protein glycosylation pathway in the pathogen Campylobacter jejuni and its subsequent transfer into laboratory strains of Escherichia coli has spurred great interest in glycoprotein production in prokaryotes.
31008057	10	78	theme	glycosylation	1681:1693	arg1	platform					1695:1702	a next-generation microbial glycosylation platform	1653:1702	a next-generation microbial glycosylation platform	1653:1702	Overall, this study demonstrates the promising role that metabolic modeling can play in optimizing the performance of a next-generation microbial glycosylation platform.
31008057	8	79	theme	overproducing	1230:1242	arg1	strains					1244:1250	these growth-coupled glycan overproducing strains	1202:1250	these growth-coupled glycan overproducing strains	1202:1250	Simulations suggested that these growth-coupled glycan overproducing strains had metabolic imbalances that rerouted flux toward glycan precursor synthesis.
31008057	1	80	theme	N-linked	120:127	arg1	glycosylation					130:142	Asparagine-linked (N-linked) glycosylation	101:142	Asparagine-linked (N-linked) glycosylation	101:142	Asparagine-linked (N-linked) glycosylation is the most common protein modification in eukaryotes, affecting over two-thirds of the proteome.
31008057	1	80	theme	N-linked	120:127	arg1	modification					171:182	the most common protein modification	147:182	the most common protein modification in eukaryotes	147:196	Asparagine-linked (N-linked) glycosylation is the most common protein modification in eukaryotes, affecting over two-thirds of the proteome.
31008057	3	81	theme	laboratory	520:529	arg1	strains					531:537	laboratory strains	520:537	laboratory strains of Escherichia coli	520:557	The discovery of a protein glycosylation pathway in the pathogen Campylobacter jejuni and its subsequent transfer into laboratory strains of Escherichia coli has spurred great interest in glycoprotein production in prokaryotes.
31008057	3	82	theme	pathogen	457:464	arg1	jejuni					480:485	the pathogen Campylobacter jejuni	453:485	the pathogen Campylobacter jejuni	453:485	The discovery of a protein glycosylation pathway in the pathogen Campylobacter jejuni and its subsequent transfer into laboratory strains of Escherichia coli has spurred great interest in glycoprotein production in prokaryotes.
31008057	3	83	theme	transfer	506:513	arg1	discovery					405:413	The discovery	401:413	The discovery of a protein glycosylation pathway in the pathogen Campylobacter jejuni and its subsequent transfer into laboratory strains of Escherichia coli	401:557	The discovery of a protein glycosylation pathway in the pathogen Campylobacter jejuni and its subsequent transfer into laboratory strains of Escherichia coli has spurred great interest in glycoprotein production in prokaryotes.
31008057	5	84	theme	design	895:900	arg1	strains					916:922	design gene knockout strains	895:922	design gene knockout strains that overproduced glycan precursors	895:958	To address this limitation, we used a constraint-based model of E. coli metabolism in combination with heuristic optimization to design gene knockout strains that overproduced glycan precursors.
31008057	3	85	theme	coli	554:557	arg1	strains					531:537	laboratory strains	520:537	laboratory strains of Escherichia coli	520:557	The discovery of a protein glycosylation pathway in the pathogen Campylobacter jejuni and its subsequent transfer into laboratory strains of Escherichia coli has spurred great interest in glycoprotein production in prokaryotes.
31008057	8	86	contain	had	1252:1254	arg1	strains					1244:1250	these growth-coupled glycan overproducing strains	1202:1250	these growth-coupled glycan overproducing strains	1202:1250	Simulations suggested that these growth-coupled glycan overproducing strains had metabolic imbalances that rerouted flux toward glycan precursor synthesis.
31008057	8	86	contain	had	1252:1254	arg2	imbalances					1266:1275	metabolic imbalances	1256:1275	metabolic imbalances that rerouted flux toward glycan precursor synthesis	1256:1328	Simulations suggested that these growth-coupled glycan overproducing strains had metabolic imbalances that rerouted flux toward glycan precursor synthesis.
31008057	10	87	theme	promising	1572:1580	arg1	role					1582:1585	the promising role	1568:1585	the promising role	1568:1585	Overall, this study demonstrates the promising role that metabolic modeling can play in optimizing the performance of a next-generation microbial glycosylation platform.
31008057	0	88	theme	designer	10:17	arg1	production					26:35	designer glycan production	10:35	designer glycan production in Escherichia coli	10:55	Improving designer glycan production in Escherichia coli through model-guided metabolic engineering.
30798070	5	0	theme	high	833:836	arg1	temperature					838:848	high temperature	833:848	high temperature	833:848	However, highly sialylated N-glycopeptides containing hydrophobic amino acids exhibited increased retention times at high temperature.
30798070	3	1	theme	O-	516:517	arg1	isomers					545:551	sialylated O- and N-linked glycopeptide isomers	505:551	sialylated O- and N-linked glycopeptide isomers with decreased retention times	505:582	In general, high column temperatures enhanced the resolution for separation of sialylated O- and N-linked glycopeptide isomers with decreased retention times.
30798070	4	2	theme	RPLC	612:615	arg1	method					617:622	the high-temperature RPLC method	591:622	the high-temperature RPLC method	591:622	Using the high-temperature RPLC method, α2-6-linked sialylated N-glycopeptides were eluted first, followed by α2-3-linked isomers.
30798070	6	3	theme	N-glycopeptides	887:901	arg1	separation					855:864	The separation	851:864	The separation of sialylated O- and N-glycopeptides with different glycan isoforms using a high-temperature RPLC method	851:969	The separation of sialylated O- and N-glycopeptides with different glycan isoforms using a high-temperature RPLC method was demonstrated.
30798070	1	4	theme	peptide	257:263	arg1	backbone					265:272	the peptide backbone	253:272	the peptide backbone	253:272	Analyses of intact glycopeptides using mass spectrometry is challenging due to the numerous types of isomers of glycan moieties attached to the peptide backbone.
30798070	5	5	gly	N-glycopeptides	743:757	arg2	N-glycopeptides					743:757	highly sialylated N-glycopeptides	725:757	highly sialylated N-glycopeptides containing hydrophobic amino acids	725:792	However, highly sialylated N-glycopeptides containing hydrophobic amino acids exhibited increased retention times at high temperature.
30798070	4	6	gly	N-glycopeptides	648:662	arg2	N-glycopeptides					648:662	α2-6-linked sialylated N-glycopeptides	625:662	α2-6-linked sialylated N-glycopeptides	625:662	Using the high-temperature RPLC method, α2-6-linked sialylated N-glycopeptides were eluted first, followed by α2-3-linked isomers.
30798070	3	7	gly	glycopeptide	532:543	arg2	glycopeptide					532:543	sialylated O- and N-linked glycopeptide isomers	505:551	sialylated O- and N-linked glycopeptide isomers with decreased retention times	505:582	In general, high column temperatures enhanced the resolution for separation of sialylated O- and N-linked glycopeptide isomers with decreased retention times.
30798070	4	8	theme	α2-3-linked	695:705	arg1	isomers					707:713	α2-3-linked isomers	695:713	α2-3-linked isomers	695:713	Using the high-temperature RPLC method, α2-6-linked sialylated N-glycopeptides were eluted first, followed by α2-3-linked isomers.
30798070	7	9	from	temperatures	1072:1083	arg1	separation					1046:1055	reversed-phase chromatographic separation	1015:1055	reversed-phase chromatographic separation at high column temperatures	1015:1083	This study indicates that reversed-phase chromatographic separation at high column temperatures is suitable for the separation of glycopeptide structural isomers.
30798070	0	10	theme	high	95:98	arg1	temperature					100:110	high temperature	95:110	high temperature	95:110	Isomer separation of sialylated O- and N-linked glycopeptides using reversed-phase LC-MS/MS at high temperature.
30798070	2	11	gly	glycopeptides	411:423	arg2	glycopeptides					411:423	separate isomeric O- and N-linked glycopeptides	377:423	separate isomeric O- and N-linked glycopeptides	377:423	Here, we demonstrate that high-temperature reversed-phase liquid chromatography (RPLC) can be used to separate isomeric O- and N-linked glycopeptides.
30798070	2	12	used	used	369:372	arg2	chromatography					340:353	high-temperature reversed-phase liquid chromatography	301:353	high-temperature reversed-phase liquid chromatography (RPLC)	301:360	Here, we demonstrate that high-temperature reversed-phase liquid chromatography (RPLC) can be used to separate isomeric O- and N-linked glycopeptides.
30798070	2	12	used	used	369:372	arg2	RPLC					356:359	RPLC	356:359	RPLC	356:359	Here, we demonstrate that high-temperature reversed-phase liquid chromatography (RPLC) can be used to separate isomeric O- and N-linked glycopeptides.
30798070	3	13	theme	retention	568:576	arg1	times					578:582	decreased retention times	558:582	decreased retention times	558:582	In general, high column temperatures enhanced the resolution for separation of sialylated O- and N-linked glycopeptide isomers with decreased retention times.
30798070	7	14	theme	column	1065:1070	arg1	temperatures					1072:1083	high column temperatures	1060:1083	high column temperatures	1060:1083	This study indicates that reversed-phase chromatographic separation at high column temperatures is suitable for the separation of glycopeptide structural isomers.
30798070	5	15	theme	increased	804:812	arg1	times					824:828	increased retention times	804:828	increased retention times	804:828	However, highly sialylated N-glycopeptides containing hydrophobic amino acids exhibited increased retention times at high temperature.
30798070	4	16	dep	eluted	669:674	arg1	followed					683:690	followed	683:690	followed by α2-3-linked isomers	683:713	Using the high-temperature RPLC method, α2-6-linked sialylated N-glycopeptides were eluted first, followed by α2-3-linked isomers.
30798070	6	17	theme	sialylated	869:878	arg1	O-					880:881	O-	880:881	O-	880:881	The separation of sialylated O- and N-glycopeptides with different glycan isoforms using a high-temperature RPLC method was demonstrated.
30798070	3	18	theme	isomers	545:551	arg1	separation					491:500	separation	491:500	separation of sialylated O- and N-linked glycopeptide isomers with decreased retention times	491:582	In general, high column temperatures enhanced the resolution for separation of sialylated O- and N-linked glycopeptide isomers with decreased retention times.
30798070	3	19	theme	high	438:441	arg1	temperatures					450:461	high column temperatures	438:461	high column temperatures	438:461	In general, high column temperatures enhanced the resolution for separation of sialylated O- and N-linked glycopeptide isomers with decreased retention times.
30798070	5	20	theme	retention	814:822	arg1	times					824:828	increased retention times	804:828	increased retention times	804:828	However, highly sialylated N-glycopeptides containing hydrophobic amino acids exhibited increased retention times at high temperature.
30798070	4	21	theme	α2-6-linked	625:635	arg1	N-glycopeptides					648:662	α2-6-linked sialylated N-glycopeptides	625:662	α2-6-linked sialylated N-glycopeptides	625:662	Using the high-temperature RPLC method, α2-6-linked sialylated N-glycopeptides were eluted first, followed by α2-3-linked isomers.
30798070	2	22	theme	O-	395:396	arg1	glycopeptides					411:423	separate isomeric O- and N-linked glycopeptides	377:423	separate isomeric O- and N-linked glycopeptides	377:423	Here, we demonstrate that high-temperature reversed-phase liquid chromatography (RPLC) can be used to separate isomeric O- and N-linked glycopeptides.
30798070	6	23	with	O-	880:881	arg1	isoforms					925:932	different glycan isoforms	908:932	different glycan isoforms using a high-temperature RPLC method	908:969	The separation of sialylated O- and N-glycopeptides with different glycan isoforms using a high-temperature RPLC method was demonstrated.
30798070	0	24	link	N-linked	39:46	arg1	glycopeptides					48:60	sialylated O- and N-linked glycopeptides	21:60	sialylated O- and N-linked glycopeptides using reversed-phase LC-MS/MS at high temperature	21:110	Isomer separation of sialylated O- and N-linked glycopeptides using reversed-phase LC-MS/MS at high temperature.
30798070	2	25	theme	liquid	333:338	arg1	RPLC					356:359	RPLC	356:359	RPLC	356:359	Here, we demonstrate that high-temperature reversed-phase liquid chromatography (RPLC) can be used to separate isomeric O- and N-linked glycopeptides.
30798070	2	25	theme	liquid	333:338	arg1	chromatography					340:353	high-temperature reversed-phase liquid chromatography	301:353	high-temperature reversed-phase liquid chromatography (RPLC)	301:360	Here, we demonstrate that high-temperature reversed-phase liquid chromatography (RPLC) can be used to separate isomeric O- and N-linked glycopeptides.
30798070	2	26	theme	isomeric	386:393	arg1	glycopeptides					411:423	separate isomeric O- and N-linked glycopeptides	377:423	separate isomeric O- and N-linked glycopeptides	377:423	Here, we demonstrate that high-temperature reversed-phase liquid chromatography (RPLC) can be used to separate isomeric O- and N-linked glycopeptides.
30798070	0	27	theme	Isomer	0:5	arg1	separation					7:16	Isomer separation	0:16	Isomer separation of sialylated O- and N-linked glycopeptides using reversed-phase LC-MS/MS at high temperature.	0:111	Isomer separation of sialylated O- and N-linked glycopeptides using reversed-phase LC-MS/MS at high temperature.
30798070	5	28	theme	hydrophobic	770:780	arg1	acids					788:792	hydrophobic amino acids	770:792	hydrophobic amino acids	770:792	However, highly sialylated N-glycopeptides containing hydrophobic amino acids exhibited increased retention times at high temperature.
30798070	7	29	theme	reversed-phase	1015:1028	arg1	separation					1046:1055	reversed-phase chromatographic separation	1015:1055	reversed-phase chromatographic separation at high column temperatures	1015:1083	This study indicates that reversed-phase chromatographic separation at high column temperatures is suitable for the separation of glycopeptide structural isomers.
30798070	2	30	theme	reversed-phase	318:331	arg1	RPLC					356:359	RPLC	356:359	RPLC	356:359	Here, we demonstrate that high-temperature reversed-phase liquid chromatography (RPLC) can be used to separate isomeric O- and N-linked glycopeptides.
30798070	2	30	theme	reversed-phase	318:331	arg1	chromatography					340:353	high-temperature reversed-phase liquid chromatography	301:353	high-temperature reversed-phase liquid chromatography (RPLC)	301:360	Here, we demonstrate that high-temperature reversed-phase liquid chromatography (RPLC) can be used to separate isomeric O- and N-linked glycopeptides.
30798070	6	31	theme	glycan	918:923	arg1	isoforms					925:932	different glycan isoforms	908:932	different glycan isoforms using a high-temperature RPLC method	908:969	The separation of sialylated O- and N-glycopeptides with different glycan isoforms using a high-temperature RPLC method was demonstrated.
30798070	1	32	theme	numerous	196:203	arg1	types					205:209	the numerous types	192:209	the numerous types of isomers of glycan moieties attached to the peptide backbone	192:272	Analyses of intact glycopeptides using mass spectrometry is challenging due to the numerous types of isomers of glycan moieties attached to the peptide backbone.
30798070	0	33	theme	sialylated	21:30	arg1	glycopeptides					48:60	sialylated O- and N-linked glycopeptides	21:60	sialylated O- and N-linked glycopeptides using reversed-phase LC-MS/MS at high temperature	21:110	Isomer separation of sialylated O- and N-linked glycopeptides using reversed-phase LC-MS/MS at high temperature.
30798070	7	34	theme	high	1060:1063	arg1	temperatures					1072:1083	high column temperatures	1060:1083	high column temperatures	1060:1083	This study indicates that reversed-phase chromatographic separation at high column temperatures is suitable for the separation of glycopeptide structural isomers.
30798070	7	35	gly	glycopeptide	1119:1130	arg2	glycopeptide					1119:1130	glycopeptide	1119:1130	glycopeptide	1119:1130	This study indicates that reversed-phase chromatographic separation at high column temperatures is suitable for the separation of glycopeptide structural isomers.
30798070	0	36	gly	sialylated	21:30	arg1	glycopeptides					48:60	sialylated O- and N-linked glycopeptides	21:60	sialylated O- and N-linked glycopeptides using reversed-phase LC-MS/MS at high temperature	21:110	Isomer separation of sialylated O- and N-linked glycopeptides using reversed-phase LC-MS/MS at high temperature.
30798070	2	37	theme	high-temperature	301:316	arg1	RPLC					356:359	RPLC	356:359	RPLC	356:359	Here, we demonstrate that high-temperature reversed-phase liquid chromatography (RPLC) can be used to separate isomeric O- and N-linked glycopeptides.
30798070	2	37	theme	high-temperature	301:316	arg1	chromatography					340:353	high-temperature reversed-phase liquid chromatography	301:353	high-temperature reversed-phase liquid chromatography (RPLC)	301:360	Here, we demonstrate that high-temperature reversed-phase liquid chromatography (RPLC) can be used to separate isomeric O- and N-linked glycopeptides.
30798070	5	38	contain	containing	759:768	arg2	acids					788:792	hydrophobic amino acids	770:792	hydrophobic amino acids	770:792	However, highly sialylated N-glycopeptides containing hydrophobic amino acids exhibited increased retention times at high temperature.
30798070	5	38	contain	containing	759:768	arg1	N-glycopeptides					743:757	highly sialylated N-glycopeptides	725:757	highly sialylated N-glycopeptides containing hydrophobic amino acids	725:792	However, highly sialylated N-glycopeptides containing hydrophobic amino acids exhibited increased retention times at high temperature.
30798070	4	39	theme	sialylated	637:646	arg1	N-glycopeptides					648:662	α2-6-linked sialylated N-glycopeptides	625:662	α2-6-linked sialylated N-glycopeptides	625:662	Using the high-temperature RPLC method, α2-6-linked sialylated N-glycopeptides were eluted first, followed by α2-3-linked isomers.
30798070	6	40	theme	RPLC	959:962	arg1	method					964:969	a high-temperature RPLC method	940:969	a high-temperature RPLC method	940:969	The separation of sialylated O- and N-glycopeptides with different glycan isoforms using a high-temperature RPLC method was demonstrated.
30798070	5	41	gly	sialylated	732:741	arg1	N-glycopeptides					743:757	highly sialylated N-glycopeptides	725:757	highly sialylated N-glycopeptides containing hydrophobic amino acids	725:792	However, highly sialylated N-glycopeptides containing hydrophobic amino acids exhibited increased retention times at high temperature.
30798070	4	42	theme	high-temperature	595:610	arg1	method					617:622	the high-temperature RPLC method	591:622	the high-temperature RPLC method	591:622	Using the high-temperature RPLC method, α2-6-linked sialylated N-glycopeptides were eluted first, followed by α2-3-linked isomers.
30798070	3	43	theme	N-linked	523:530	arg1	isomers					545:551	sialylated O- and N-linked glycopeptide isomers	505:551	sialylated O- and N-linked glycopeptide isomers with decreased retention times	505:582	In general, high column temperatures enhanced the resolution for separation of sialylated O- and N-linked glycopeptide isomers with decreased retention times.
30798070	3	44	with	isomers	545:551	arg1	times					578:582	decreased retention times	558:582	decreased retention times	558:582	In general, high column temperatures enhanced the resolution for separation of sialylated O- and N-linked glycopeptide isomers with decreased retention times.
30798070	3	45	theme	sialylated	505:514	arg1	isomers					545:551	sialylated O- and N-linked glycopeptide isomers	505:551	sialylated O- and N-linked glycopeptide isomers with decreased retention times	505:582	In general, high column temperatures enhanced the resolution for separation of sialylated O- and N-linked glycopeptide isomers with decreased retention times.
30798070	3	46	link	N-linked	523:530	arg1	isomers					545:551	sialylated O- and N-linked glycopeptide isomers	505:551	sialylated O- and N-linked glycopeptide isomers with decreased retention times	505:582	In general, high column temperatures enhanced the resolution for separation of sialylated O- and N-linked glycopeptide isomers with decreased retention times.
30798070	6	47	theme	high-temperature	942:957	arg1	method					964:969	a high-temperature RPLC method	940:969	a high-temperature RPLC method	940:969	The separation of sialylated O- and N-glycopeptides with different glycan isoforms using a high-temperature RPLC method was demonstrated.
30798070	5	48	theme	amino	782:786	arg1	acids					788:792	hydrophobic amino acids	770:792	hydrophobic amino acids	770:792	However, highly sialylated N-glycopeptides containing hydrophobic amino acids exhibited increased retention times at high temperature.
30798070	2	49	theme	separate	377:384	arg1	glycopeptides					411:423	separate isomeric O- and N-linked glycopeptides	377:423	separate isomeric O- and N-linked glycopeptides	377:423	Here, we demonstrate that high-temperature reversed-phase liquid chromatography (RPLC) can be used to separate isomeric O- and N-linked glycopeptides.
30798070	1	50	theme	isomers	214:220	arg1	types					205:209	the numerous types	192:209	the numerous types of isomers of glycan moieties attached to the peptide backbone	192:272	Analyses of intact glycopeptides using mass spectrometry is challenging due to the numerous types of isomers of glycan moieties attached to the peptide backbone.
30798070	0	51	theme	O-	32:33	arg1	glycopeptides					48:60	sialylated O- and N-linked glycopeptides	21:60	sialylated O- and N-linked glycopeptides using reversed-phase LC-MS/MS at high temperature	21:110	Isomer separation of sialylated O- and N-linked glycopeptides using reversed-phase LC-MS/MS at high temperature.
30798070	1	52	gly	glycopeptides	132:144	arg2	glycopeptides					132:144	intact glycopeptides	125:144	intact glycopeptides using mass spectrometry	125:168	Analyses of intact glycopeptides using mass spectrometry is challenging due to the numerous types of isomers of glycan moieties attached to the peptide backbone.
30798070	6	53	with	N-glycopeptides	887:901	arg1	isoforms					925:932	different glycan isoforms	908:932	different glycan isoforms using a high-temperature RPLC method	908:969	The separation of sialylated O- and N-glycopeptides with different glycan isoforms using a high-temperature RPLC method was demonstrated.
30798070	7	54	theme	structural	1132:1141	arg1	isomers					1143:1149	glycopeptide structural isomers	1119:1149	glycopeptide structural isomers	1119:1149	This study indicates that reversed-phase chromatographic separation at high column temperatures is suitable for the separation of glycopeptide structural isomers.
30798070	2	55	theme	N-linked	402:409	arg1	glycopeptides					411:423	separate isomeric O- and N-linked glycopeptides	377:423	separate isomeric O- and N-linked glycopeptides	377:423	Here, we demonstrate that high-temperature reversed-phase liquid chromatography (RPLC) can be used to separate isomeric O- and N-linked glycopeptides.
30798070	0	56	theme	glycopeptides	48:60	arg1	separation					7:16	Isomer separation	0:16	Isomer separation of sialylated O- and N-linked glycopeptides using reversed-phase LC-MS/MS at high temperature.	0:111	Isomer separation of sialylated O- and N-linked glycopeptides using reversed-phase LC-MS/MS at high temperature.
30798070	6	57	gly	N-glycopeptides	887:901	arg2	N-glycopeptides					887:901	N-glycopeptides	887:901	N-glycopeptides	887:901	The separation of sialylated O- and N-glycopeptides with different glycan isoforms using a high-temperature RPLC method was demonstrated.
30798070	6	58	theme	O-	880:881	arg1	separation					855:864	The separation	851:864	The separation of sialylated O- and N-glycopeptides with different glycan isoforms using a high-temperature RPLC method	851:969	The separation of sialylated O- and N-glycopeptides with different glycan isoforms using a high-temperature RPLC method was demonstrated.
30798070	4	59	link	α2-6-linked	625:635	arg1	N-glycopeptides					648:662	α2-6-linked sialylated N-glycopeptides	625:662	α2-6-linked sialylated N-glycopeptides	625:662	Using the high-temperature RPLC method, α2-6-linked sialylated N-glycopeptides were eluted first, followed by α2-3-linked isomers.
30798070	4	60	link	α2-3-linked	695:705	arg1	isomers					707:713	α2-3-linked isomers	695:713	α2-3-linked isomers	695:713	Using the high-temperature RPLC method, α2-6-linked sialylated N-glycopeptides were eluted first, followed by α2-3-linked isomers.
30798070	7	61	theme	isomers	1143:1149	arg1	separation					1105:1114	the separation	1101:1114	the separation of glycopeptide structural isomers	1101:1149	This study indicates that reversed-phase chromatographic separation at high column temperatures is suitable for the separation of glycopeptide structural isomers.
30798070	0	62	theme	N-linked	39:46	arg1	glycopeptides					48:60	sialylated O- and N-linked glycopeptides	21:60	sialylated O- and N-linked glycopeptides using reversed-phase LC-MS/MS at high temperature	21:110	Isomer separation of sialylated O- and N-linked glycopeptides using reversed-phase LC-MS/MS at high temperature.
30798070	6	63	gly	sialylated	869:878	arg1	O-					880:881	O-	880:881	O-	880:881	The separation of sialylated O- and N-glycopeptides with different glycan isoforms using a high-temperature RPLC method was demonstrated.
30798070	1	64	attach	attached	241:248	arg1	backbone					265:272	the peptide backbone	253:272	the peptide backbone	253:272	Analyses of intact glycopeptides using mass spectrometry is challenging due to the numerous types of isomers of glycan moieties attached to the peptide backbone.
30798070	1	64	attach	attached	241:248	arg2	moieties					232:239	glycan moieties	225:239	glycan moieties attached to the peptide backbone	225:272	Analyses of intact glycopeptides using mass spectrometry is challenging due to the numerous types of isomers of glycan moieties attached to the peptide backbone.
30798070	1	65	theme	intact	125:130	arg1	glycopeptides					132:144	intact glycopeptides	125:144	intact glycopeptides using mass spectrometry	125:168	Analyses of intact glycopeptides using mass spectrometry is challenging due to the numerous types of isomers of glycan moieties attached to the peptide backbone.
30798070	7	66	theme	chromatographic	1030:1044	arg1	separation					1046:1055	reversed-phase chromatographic separation	1015:1055	reversed-phase chromatographic separation at high column temperatures	1015:1083	This study indicates that reversed-phase chromatographic separation at high column temperatures is suitable for the separation of glycopeptide structural isomers.
30798070	2	67	link	N-linked	402:409	arg1	glycopeptides					411:423	separate isomeric O- and N-linked glycopeptides	377:423	separate isomeric O- and N-linked glycopeptides	377:423	Here, we demonstrate that high-temperature reversed-phase liquid chromatography (RPLC) can be used to separate isomeric O- and N-linked glycopeptides.
30798070	4	68	gly	sialylated	637:646	arg1	N-glycopeptides					648:662	α2-6-linked sialylated N-glycopeptides	625:662	α2-6-linked sialylated N-glycopeptides	625:662	Using the high-temperature RPLC method, α2-6-linked sialylated N-glycopeptides were eluted first, followed by α2-3-linked isomers.
30798070	1	69	theme	glycopeptides	132:144	arg1	Analyses					113:120	Analyses	113:120	Analyses of intact glycopeptides using mass spectrometry	113:168	Analyses of intact glycopeptides using mass spectrometry is challenging due to the numerous types of isomers of glycan moieties attached to the peptide backbone.
30798070	1	70	theme	glycan	225:230	arg1	moieties					232:239	glycan moieties	225:239	glycan moieties attached to the peptide backbone	225:272	Analyses of intact glycopeptides using mass spectrometry is challenging due to the numerous types of isomers of glycan moieties attached to the peptide backbone.
30798070	5	71	theme	sialylated	732:741	arg1	N-glycopeptides					743:757	highly sialylated N-glycopeptides	725:757	highly sialylated N-glycopeptides containing hydrophobic amino acids	725:792	However, highly sialylated N-glycopeptides containing hydrophobic amino acids exhibited increased retention times at high temperature.
30798070	6	72	theme	different	908:916	arg1	isoforms					925:932	different glycan isoforms	908:932	different glycan isoforms using a high-temperature RPLC method	908:969	The separation of sialylated O- and N-glycopeptides with different glycan isoforms using a high-temperature RPLC method was demonstrated.
30798070	3	73	theme	decreased	558:566	arg1	times					578:582	decreased retention times	558:582	decreased retention times	558:582	In general, high column temperatures enhanced the resolution for separation of sialylated O- and N-linked glycopeptide isomers with decreased retention times.
30798070	3	74	gly	sialylated	505:514	arg1	isomers					545:551	sialylated O- and N-linked glycopeptide isomers	505:551	sialylated O- and N-linked glycopeptide isomers with decreased retention times	505:582	In general, high column temperatures enhanced the resolution for separation of sialylated O- and N-linked glycopeptide isomers with decreased retention times.
30798070	1	75	theme	moieties	232:239	arg1	isomers					214:220	isomers	214:220	isomers of glycan moieties attached to the peptide backbone	214:272	Analyses of intact glycopeptides using mass spectrometry is challenging due to the numerous types of isomers of glycan moieties attached to the peptide backbone.
30798070	0	76	theme	reversed-phase	68:81	arg1	LC-MS/MS					83:90	reversed-phase LC-MS/MS	68:90	reversed-phase LC-MS/MS	68:90	Isomer separation of sialylated O- and N-linked glycopeptides using reversed-phase LC-MS/MS at high temperature.
30798070	7	77	theme	glycopeptide	1119:1130	arg1	isomers					1143:1149	glycopeptide structural isomers	1119:1149	glycopeptide structural isomers	1119:1149	This study indicates that reversed-phase chromatographic separation at high column temperatures is suitable for the separation of glycopeptide structural isomers.
30798070	1	78	theme	mass	152:155	arg1	spectrometry					157:168	mass spectrometry	152:168	mass spectrometry	152:168	Analyses of intact glycopeptides using mass spectrometry is challenging due to the numerous types of isomers of glycan moieties attached to the peptide backbone.
30798070	3	79	theme	column	443:448	arg1	temperatures					450:461	high column temperatures	438:461	high column temperatures	438:461	In general, high column temperatures enhanced the resolution for separation of sialylated O- and N-linked glycopeptide isomers with decreased retention times.
30798070	0	80	gly	glycopeptides	48:60	arg2	glycopeptides					48:60	sialylated O- and N-linked glycopeptides	21:60	sialylated O- and N-linked glycopeptides using reversed-phase LC-MS/MS at high temperature	21:110	Isomer separation of sialylated O- and N-linked glycopeptides using reversed-phase LC-MS/MS at high temperature.
30798070	3	81	theme	glycopeptide	532:543	arg1	isomers					545:551	sialylated O- and N-linked glycopeptide isomers	505:551	sialylated O- and N-linked glycopeptide isomers with decreased retention times	505:582	In general, high column temperatures enhanced the resolution for separation of sialylated O- and N-linked glycopeptide isomers with decreased retention times.
30515991	5	0	theme	decreased	746:754	arg1	interaction					756:766	decreased interaction	746:766	decreased interaction between c-Fos and O-GlcNAcase	746:796	We found that amyloid beta (Aβ)-induced O-GlcNAcylation on serine-56 and 57 of c-Fos was resulted from decreased interaction between c-Fos and O-GlcNAcase and promoted neuronal cell death.
30515991	7	1	theme	Aβ-induced	1050:1059	arg1	O-GlcNAcylation					1061:1075	Aβ-induced O-GlcNAcylation	1050:1075	Aβ-induced O-GlcNAcylation of c-Fos	1050:1084	Taken together, Aβ-induced O-GlcNAcylation of c-Fos plays an important role in neuronal cell death during the pathogenesis of AD.
30515991	2	2	theme	patients	369:376	arg1	brains					359:364	the brains	355:364	the brains of patients with AD	355:384	O-linked β-N-acetyl glucosamine (O-GlcNAc) modification of the proteins reflecting glucose metabolism is altered in the brains of patients with AD.
30515991	4	3	theme	O-GlcNAcylated	575:588	arg1	c-Fos					590:594	O-GlcNAcylated c-Fos	575:594	O-GlcNAcylated c-Fos	575:594	Here, we examined the regulation of O-GlcNAcylation of c-Fos and the effects of O-GlcNAcylated c-Fos on neuronal cell death during AD pathogenesis.
30515991	6	4	theme	higher	930:935	arg1	interaction					937:947	higher interaction	930:947	higher interaction	930:947	O-GlcNAcylated c-Fos increased its stability and potentiated the transcriptional activity through higher interaction with c-Jun, resulting in induction of Bim expression leading to neuronal cell death.
30515991	2	5	mod	modification	282:293	arg1	proteins					302:309	the proteins	298:309	the proteins reflecting glucose metabolism	298:339	O-linked β-N-acetyl glucosamine (O-GlcNAc) modification of the proteins reflecting glucose metabolism is altered in the brains of patients with AD.
30515991	2	5	mod	modification	282:293	arg3	glucosamine					259:269	O-linked β-N-acetyl glucosamine (O-GlcNAc) modification	239:293	O-linked β-N-acetyl glucosamine (O-GlcNAc) modification of the proteins reflecting glucose metabolism	239:339	O-linked β-N-acetyl glucosamine (O-GlcNAc) modification of the proteins reflecting glucose metabolism is altered in the brains of patients with AD.
30515991	2	5	mod	modification	282:293	arg3	O-GlcNAc					272:279	O-linked β-N-acetyl glucosamine (O-GlcNAc) modification	239:293	O-linked β-N-acetyl glucosamine (O-GlcNAc) modification of the proteins reflecting glucose metabolism	239:339	O-linked β-N-acetyl glucosamine (O-GlcNAc) modification of the proteins reflecting glucose metabolism is altered in the brains of patients with AD.
30515991	6	6	theme	neuronal	1013:1020	arg1	death					1027:1031	neuronal cell death	1013:1031	neuronal cell death	1013:1031	O-GlcNAcylated c-Fos increased its stability and potentiated the transcriptional activity through higher interaction with c-Jun, resulting in induction of Bim expression leading to neuronal cell death.
30515991	5	7	theme	amyloid	657:663	arg1	beta					665:668	amyloid beta	657:668	amyloid beta	657:668	We found that amyloid beta (Aβ)-induced O-GlcNAcylation on serine-56 and 57 of c-Fos was resulted from decreased interaction between c-Fos and O-GlcNAcase and promoted neuronal cell death.
30515991	2	8	theme	proteins	302:309	arg1	modification					282:293	O-linked β-N-acetyl glucosamine (O-GlcNAc) modification	239:293	O-linked β-N-acetyl glucosamine (O-GlcNAc) modification of the proteins reflecting glucose metabolism	239:339	O-linked β-N-acetyl glucosamine (O-GlcNAc) modification of the proteins reflecting glucose metabolism is altered in the brains of patients with AD.
30515991	5	9	from	O-GlcNAcylation	683:697	arg1	57					716:717	57	716:717	57	716:717	We found that amyloid beta (Aβ)-induced O-GlcNAcylation on serine-56 and 57 of c-Fos was resulted from decreased interaction between c-Fos and O-GlcNAcase and promoted neuronal cell death.
30515991	5	9	from	O-GlcNAcylation	683:697	arg1	serine-56					702:710	serine-56	702:710	serine-56	702:710	We found that amyloid beta (Aβ)-induced O-GlcNAcylation on serine-56 and 57 of c-Fos was resulted from decreased interaction between c-Fos and O-GlcNAcase and promoted neuronal cell death.
30515991	5	10	dep	-induced	674:681	arg1	beta					665:668	amyloid beta	657:668	amyloid beta	657:668	We found that amyloid beta (Aβ)-induced O-GlcNAcylation on serine-56 and 57 of c-Fos was resulted from decreased interaction between c-Fos and O-GlcNAcase and promoted neuronal cell death.
30515991	4	11	theme	c-Fos	590:594	arg1	regulation					517:526	the regulation	513:526	the regulation of O-GlcNAcylation of c-Fos	513:554	Here, we examined the regulation of O-GlcNAcylation of c-Fos and the effects of O-GlcNAcylated c-Fos on neuronal cell death during AD pathogenesis.
30515991	4	11	theme	c-Fos	590:594	arg1	effects					564:570	the effects	560:570	the effects of O-GlcNAcylated c-Fos on neuronal cell death	560:617	Here, we examined the regulation of O-GlcNAcylation of c-Fos and the effects of O-GlcNAcylated c-Fos on neuronal cell death during AD pathogenesis.
30515991	5	12	theme	c-Fos	722:726	arg1	O-GlcNAcylation					683:697	amyloid beta (Aβ)-induced O-GlcNAcylation	657:697	amyloid beta (Aβ)-induced O-GlcNAcylation on serine-56 and 57 of c-Fos	657:726	We found that amyloid beta (Aβ)-induced O-GlcNAcylation on serine-56 and 57 of c-Fos was resulted from decreased interaction between c-Fos and O-GlcNAcase and promoted neuronal cell death.
30515991	6	13	theme	transcriptional	897:911	arg1	activity					913:920	the transcriptional activity	893:920	the transcriptional activity	893:920	O-GlcNAcylated c-Fos increased its stability and potentiated the transcriptional activity through higher interaction with c-Jun, resulting in induction of Bim expression leading to neuronal cell death.
30515991	7	14	theme	neuronal	1113:1120	arg1	death					1127:1131	neuronal cell death	1113:1131	neuronal cell death	1113:1131	Taken together, Aβ-induced O-GlcNAcylation of c-Fos plays an important role in neuronal cell death during the pathogenesis of AD.
30515991	4	15	theme	AD	626:627	arg1	pathogenesis					629:640	AD pathogenesis	626:640	AD pathogenesis	626:640	Here, we examined the regulation of O-GlcNAcylation of c-Fos and the effects of O-GlcNAcylated c-Fos on neuronal cell death during AD pathogenesis.
30515991	1	16	theme	progressive	168:178	arg1	loss					187:190	progressive memory loss	168:190	progressive memory loss resulting from cumulative neuronal cell death	168:236	Alzheimer's disease (AD) is an age-related neurodegenerative disease characterized by progressive memory loss resulting from cumulative neuronal cell death.
30515991	7	17	theme	AD	1160:1161	arg1	pathogenesis					1144:1155	the pathogenesis	1140:1155	the pathogenesis of AD	1140:1161	Taken together, Aβ-induced O-GlcNAcylation of c-Fos plays an important role in neuronal cell death during the pathogenesis of AD.
30515991	3	18	theme	altered	413:419	arg1	modification					430:441	altered O-GlcNAc modification	413:441	altered O-GlcNAc modification	413:441	However, the link between altered O-GlcNAc modification and neuronal cell death in AD is poorly understood.
30515991	1	19	theme	memory	180:185	arg1	loss					187:190	progressive memory loss	168:190	progressive memory loss resulting from cumulative neuronal cell death	168:236	Alzheimer's disease (AD) is an age-related neurodegenerative disease characterized by progressive memory loss resulting from cumulative neuronal cell death.
30515991	0	20	theme	β-induced	8:16	arg1	elevation					18:26	Amyloid β-induced elevation	0:26	Amyloid β-induced elevation of O-GlcNAcylated c-Fos	0:50	Amyloid β-induced elevation of O-GlcNAcylated c-Fos promotes neuronal cell death.
30515991	6	21	with	interaction	937:947	arg1	c-Jun					954:958	c-Jun	954:958	c-Jun	954:958	O-GlcNAcylated c-Fos increased its stability and potentiated the transcriptional activity through higher interaction with c-Jun, resulting in induction of Bim expression leading to neuronal cell death.
30515991	0	22	theme	Amyloid	0:6	arg1	elevation					18:26	Amyloid β-induced elevation	0:26	Amyloid β-induced elevation of O-GlcNAcylated c-Fos	0:50	Amyloid β-induced elevation of O-GlcNAcylated c-Fos promotes neuronal cell death.
30515991	6	23	theme	expression	991:1000	arg1	induction					974:982	induction	974:982	induction of Bim expression leading to neuronal cell death	974:1031	O-GlcNAcylated c-Fos increased its stability and potentiated the transcriptional activity through higher interaction with c-Jun, resulting in induction of Bim expression leading to neuronal cell death.
30515991	6	24	theme	cell	1022:1025	arg1	death					1027:1031	neuronal cell death	1013:1031	neuronal cell death	1013:1031	O-GlcNAcylated c-Fos increased its stability and potentiated the transcriptional activity through higher interaction with c-Jun, resulting in induction of Bim expression leading to neuronal cell death.
30515991	5	25	theme	neuronal	811:818	arg1	death					825:829	neuronal cell death	811:829	neuronal cell death	811:829	We found that amyloid beta (Aβ)-induced O-GlcNAcylation on serine-56 and 57 of c-Fos was resulted from decreased interaction between c-Fos and O-GlcNAcase and promoted neuronal cell death.
30515991	4	26	theme	c-Fos	550:554	arg1	O-GlcNAcylation					531:545	O-GlcNAcylation	531:545	O-GlcNAcylation of c-Fos	531:554	Here, we examined the regulation of O-GlcNAcylation of c-Fos and the effects of O-GlcNAcylated c-Fos on neuronal cell death during AD pathogenesis.
30515991	2	27	theme	glucose	322:328	arg1	metabolism					330:339	glucose metabolism	322:339	glucose metabolism	322:339	O-linked β-N-acetyl glucosamine (O-GlcNAc) modification of the proteins reflecting glucose metabolism is altered in the brains of patients with AD.
30515991	5	28	theme	cell	820:823	arg1	death					825:829	neuronal cell death	811:829	neuronal cell death	811:829	We found that amyloid beta (Aβ)-induced O-GlcNAcylation on serine-56 and 57 of c-Fos was resulted from decreased interaction between c-Fos and O-GlcNAcase and promoted neuronal cell death.
30515991	7	29	theme	important	1095:1103	arg1	role					1105:1108	an important role	1092:1108	an important role	1092:1108	Taken together, Aβ-induced O-GlcNAcylation of c-Fos plays an important role in neuronal cell death during the pathogenesis of AD.
30515991	6	30	theme	Bim	987:989	arg1	expression					991:1000	Bim expression	987:1000	Bim expression leading to neuronal cell death	987:1031	O-GlcNAcylated c-Fos increased its stability and potentiated the transcriptional activity through higher interaction with c-Jun, resulting in induction of Bim expression leading to neuronal cell death.
30515991	1	31	theme	cumulative	207:216	arg1	death					232:236	cumulative neuronal cell death	207:236	cumulative neuronal cell death	207:236	Alzheimer's disease (AD) is an age-related neurodegenerative disease characterized by progressive memory loss resulting from cumulative neuronal cell death.
30515991	0	32	theme	c-Fos	46:50	arg1	elevation					18:26	Amyloid β-induced elevation	0:26	Amyloid β-induced elevation of O-GlcNAcylated c-Fos	0:50	Amyloid β-induced elevation of O-GlcNAcylated c-Fos promotes neuronal cell death.
30515991	7	33	theme	c-Fos	1080:1084	arg1	O-GlcNAcylation					1061:1075	Aβ-induced O-GlcNAcylation	1050:1075	Aβ-induced O-GlcNAcylation of c-Fos	1050:1084	Taken together, Aβ-induced O-GlcNAcylation of c-Fos plays an important role in neuronal cell death during the pathogenesis of AD.
30515991	2	34	theme	β-N-acetyl	248:257	arg1	modification					282:293	O-linked β-N-acetyl glucosamine (O-GlcNAc) modification	239:293	O-linked β-N-acetyl glucosamine (O-GlcNAc) modification of the proteins reflecting glucose metabolism	239:339	O-linked β-N-acetyl glucosamine (O-GlcNAc) modification of the proteins reflecting glucose metabolism is altered in the brains of patients with AD.
30515991	1	35	theme	neuronal	218:225	arg1	death					232:236	cumulative neuronal cell death	207:236	cumulative neuronal cell death	207:236	Alzheimer's disease (AD) is an age-related neurodegenerative disease characterized by progressive memory loss resulting from cumulative neuronal cell death.
30515991	0	36	theme	O-GlcNAcylated	31:44	arg1	c-Fos					46:50	O-GlcNAcylated c-Fos	31:50	O-GlcNAcylated c-Fos	31:50	Amyloid β-induced elevation of O-GlcNAcylated c-Fos promotes neuronal cell death.
30515991	3	37	theme	O-GlcNAc	421:428	arg1	modification					430:441	altered O-GlcNAc modification	413:441	altered O-GlcNAc modification	413:441	However, the link between altered O-GlcNAc modification and neuronal cell death in AD is poorly understood.
30515991	2	38	theme	O-linked	239:246	arg1	modification					282:293	O-linked β-N-acetyl glucosamine (O-GlcNAc) modification	239:293	O-linked β-N-acetyl glucosamine (O-GlcNAc) modification of the proteins reflecting glucose metabolism	239:339	O-linked β-N-acetyl glucosamine (O-GlcNAc) modification of the proteins reflecting glucose metabolism is altered in the brains of patients with AD.
30515991	1	39	theme	cell	227:230	arg1	death					232:236	cumulative neuronal cell death	207:236	cumulative neuronal cell death	207:236	Alzheimer's disease (AD) is an age-related neurodegenerative disease characterized by progressive memory loss resulting from cumulative neuronal cell death.
30515991	0	40	theme	neuronal	61:68	arg1	death					75:79	neuronal cell death	61:79	neuronal cell death	61:79	Amyloid β-induced elevation of O-GlcNAcylated c-Fos promotes neuronal cell death.
30515991	3	41	theme	neuronal	447:454	arg1	death					461:465	neuronal cell death	447:465	neuronal cell death	447:465	However, the link between altered O-GlcNAc modification and neuronal cell death in AD is poorly understood.
30515991	4	42	from	regulation	517:526	arg1	death					613:617	neuronal cell death	599:617	neuronal cell death	599:617	Here, we examined the regulation of O-GlcNAcylation of c-Fos and the effects of O-GlcNAcylated c-Fos on neuronal cell death during AD pathogenesis.
30515991	2	43	with	patients	369:376	arg1	AD					383:384	AD	383:384	AD	383:384	O-linked β-N-acetyl glucosamine (O-GlcNAc) modification of the proteins reflecting glucose metabolism is altered in the brains of patients with AD.
30515991	7	44	theme	cell	1122:1125	arg1	death					1127:1131	neuronal cell death	1113:1131	neuronal cell death	1113:1131	Taken together, Aβ-induced O-GlcNAcylation of c-Fos plays an important role in neuronal cell death during the pathogenesis of AD.
30515991	4	45	theme	O-GlcNAcylation	531:545	arg1	regulation					517:526	the regulation	513:526	the regulation of O-GlcNAcylation of c-Fos	513:554	Here, we examined the regulation of O-GlcNAcylation of c-Fos and the effects of O-GlcNAcylated c-Fos on neuronal cell death during AD pathogenesis.
30515991	4	45	theme	O-GlcNAcylation	531:545	arg1	effects					564:570	the effects	560:570	the effects of O-GlcNAcylated c-Fos on neuronal cell death	560:617	Here, we examined the regulation of O-GlcNAcylation of c-Fos and the effects of O-GlcNAcylated c-Fos on neuronal cell death during AD pathogenesis.
30515991	6	46	theme	O-GlcNAcylated	832:845	arg1	c-Fos					847:851	O-GlcNAcylated c-Fos	832:851	O-GlcNAcylated c-Fos	832:851	O-GlcNAcylated c-Fos increased its stability and potentiated the transcriptional activity through higher interaction with c-Jun, resulting in induction of Bim expression leading to neuronal cell death.
30515991	4	47	theme	cell	608:611	arg1	death					613:617	neuronal cell death	599:617	neuronal cell death	599:617	Here, we examined the regulation of O-GlcNAcylation of c-Fos and the effects of O-GlcNAcylated c-Fos on neuronal cell death during AD pathogenesis.
30515991	5	48	theme	-induced	674:681	arg1	O-GlcNAcylation					683:697	amyloid beta (Aβ)-induced O-GlcNAcylation	657:697	amyloid beta (Aβ)-induced O-GlcNAcylation on serine-56 and 57 of c-Fos	657:726	We found that amyloid beta (Aβ)-induced O-GlcNAcylation on serine-56 and 57 of c-Fos was resulted from decreased interaction between c-Fos and O-GlcNAcase and promoted neuronal cell death.
30515991	2	49	theme	O-GlcNAc	272:279	arg1	modification					282:293	O-linked β-N-acetyl glucosamine (O-GlcNAc) modification	239:293	O-linked β-N-acetyl glucosamine (O-GlcNAc) modification of the proteins reflecting glucose metabolism	239:339	O-linked β-N-acetyl glucosamine (O-GlcNAc) modification of the proteins reflecting glucose metabolism is altered in the brains of patients with AD.
30515991	1	50	theme	age-related	113:123	arg1	disease					143:149	an age-related neurodegenerative disease	110:149	an age-related neurodegenerative disease characterized by progressive memory loss resulting from cumulative neuronal cell death	110:236	Alzheimer's disease (AD) is an age-related neurodegenerative disease characterized by progressive memory loss resulting from cumulative neuronal cell death.
30515991	1	50	theme	age-related	113:123	arg1	disease					94:100	Alzheimer's disease	82:100	Alzheimer's disease (AD)	82:105	Alzheimer's disease (AD) is an age-related neurodegenerative disease characterized by progressive memory loss resulting from cumulative neuronal cell death.
30515991	4	51	theme	neuronal	599:606	arg1	death					613:617	neuronal cell death	599:617	neuronal cell death	599:617	Here, we examined the regulation of O-GlcNAcylation of c-Fos and the effects of O-GlcNAcylated c-Fos on neuronal cell death during AD pathogenesis.
30515991	1	52	theme	neurodegenerative	125:141	arg1	disease					143:149	an age-related neurodegenerative disease	110:149	an age-related neurodegenerative disease characterized by progressive memory loss resulting from cumulative neuronal cell death	110:236	Alzheimer's disease (AD) is an age-related neurodegenerative disease characterized by progressive memory loss resulting from cumulative neuronal cell death.
30515991	1	52	theme	neurodegenerative	125:141	arg1	disease					94:100	Alzheimer's disease	82:100	Alzheimer's disease (AD)	82:105	Alzheimer's disease (AD) is an age-related neurodegenerative disease characterized by progressive memory loss resulting from cumulative neuronal cell death.
30515991	3	53	theme	cell	456:459	arg1	death					461:465	neuronal cell death	447:465	neuronal cell death	447:465	However, the link between altered O-GlcNAc modification and neuronal cell death in AD is poorly understood.
30515991	0	54	theme	cell	70:73	arg1	death					75:79	neuronal cell death	61:79	neuronal cell death	61:79	Amyloid β-induced elevation of O-GlcNAcylated c-Fos promotes neuronal cell death.
30515991	3	55	from	link	400:403	arg1	AD					470:471	AD	470:471	AD	470:471	However, the link between altered O-GlcNAc modification and neuronal cell death in AD is poorly understood.
30515991	2	56	theme	glucosamine	259:269	arg1	modification					282:293	O-linked β-N-acetyl glucosamine (O-GlcNAc) modification	239:293	O-linked β-N-acetyl glucosamine (O-GlcNAc) modification of the proteins reflecting glucose metabolism	239:339	O-linked β-N-acetyl glucosamine (O-GlcNAc) modification of the proteins reflecting glucose metabolism is altered in the brains of patients with AD.
30515991	4	57	from	effects	564:570	arg1	death					613:617	neuronal cell death	599:617	neuronal cell death	599:617	Here, we examined the regulation of O-GlcNAcylation of c-Fos and the effects of O-GlcNAcylated c-Fos on neuronal cell death during AD pathogenesis.
30515991	2	58	link	O-linked	239:246	arg1	modification					282:293	O-linked β-N-acetyl glucosamine (O-GlcNAc) modification	239:293	O-linked β-N-acetyl glucosamine (O-GlcNAc) modification of the proteins reflecting glucose metabolism	239:339	O-linked β-N-acetyl glucosamine (O-GlcNAc) modification of the proteins reflecting glucose metabolism is altered in the brains of patients with AD.
30063825	0	0	theme	N-Linked	80:87	arg1	Glycoproteins					89:101	Natural N-Linked Glycoproteins	72:101	Natural N-Linked Glycoproteins	72:101	Efficient Assembly of Quantum Dots with Homogenous Glycans Derived from Natural N-Linked Glycoproteins.
30063825	0	1	theme	Natural	72:78	arg1	Glycoproteins					89:101	Natural N-Linked Glycoproteins	72:101	Natural N-Linked Glycoproteins	72:101	Efficient Assembly of Quantum Dots with Homogenous Glycans Derived from Natural N-Linked Glycoproteins.
30063825	4	2	dep	homogeneous	969:979	arg1	complex-type					982:993	complex-type	982:993	complex-type	982:993	Here we provide two distinct methodologies, covalent bioconjugation and self-assembly, to functionalize two types of Quantum Dots with a homogeneous, complex-type N-linked glycan terminated with a sialic acid moiety.
30063825	1	3	theme	-appended	166:174	arg1	ligands					176:182	polyethylene glycol (PEG)-appended ligands	141:182	polyethylene glycol (PEG)-appended ligands	141:182	Coating inorganic nanoparticles with polyethylene glycol (PEG)-appended ligands, as means to preserve their physical characteristics and promote steric interactions with biological systems, including enhanced aqueous solubility and reduced immunogenicity, has been explored by several groups.
30063825	3	4	from	abundant	658:665	arg1	particular					625:634	particular	625:634	particular	625:634	In particular, N-linked glycans are abundant on the surface of most serum proteins and are composed of a branched architecture that is typically characterized by a significant level of molecular heterogeneity.
30063825	5	5	theme	physical	1060:1067	arg1	characterization					1084:1099	A detailed physical and functional characterization	1049:1099	A detailed physical and functional characterization of these glycan-coated nanoparticles	1049:1136	A detailed physical and functional characterization of these glycan-coated nanoparticles has been performed.
30063825	6	6	theme	biological	1252:1261	arg1	recognition					1289:1299	lectin recognition	1282:1299	lectin recognition	1282:1299	Our findings support the potential use of such fluorescent platforms to sense glycan-involved biological processes, such as lectin recognition and sialidase-mediated hydrolysis.
30063825	6	6	theme	biological	1252:1261	arg1	processes					1263:1271	sense glycan-involved biological processes	1230:1271	sense glycan-involved biological processes	1230:1271	Our findings support the potential use of such fluorescent platforms to sense glycan-involved biological processes, such as lectin recognition and sialidase-mediated hydrolysis.
30063825	6	6	theme	biological	1252:1261	arg1	hydrolysis					1324:1333	sialidase-mediated hydrolysis	1305:1333	sialidase-mediated hydrolysis	1305:1333	Our findings support the potential use of such fluorescent platforms to sense glycan-involved biological processes, such as lectin recognition and sialidase-mediated hydrolysis.
30063825	1	7	theme	biological	274:283	arg1	systems					285:291	biological systems	274:291	biological systems	274:291	Coating inorganic nanoparticles with polyethylene glycol (PEG)-appended ligands, as means to preserve their physical characteristics and promote steric interactions with biological systems, including enhanced aqueous solubility and reduced immunogenicity, has been explored by several groups.
30063825	4	8	theme	homogeneous	969:979	arg1	glycan					1004:1009	a homogeneous, complex-type N-linked glycan	967:1009	a homogeneous, complex-type N-linked glycan terminated with a sialic acid moiety	967:1046	Here we provide two distinct methodologies, covalent bioconjugation and self-assembly, to functionalize two types of Quantum Dots with a homogeneous, complex-type N-linked glycan terminated with a sialic acid moiety.
30063825	6	9	theme	glycan-involved	1236:1250	arg1	recognition					1289:1299	lectin recognition	1282:1299	lectin recognition	1282:1299	Our findings support the potential use of such fluorescent platforms to sense glycan-involved biological processes, such as lectin recognition and sialidase-mediated hydrolysis.
30063825	6	9	theme	glycan-involved	1236:1250	arg1	processes					1263:1271	sense glycan-involved biological processes	1230:1271	sense glycan-involved biological processes	1230:1271	Our findings support the potential use of such fluorescent platforms to sense glycan-involved biological processes, such as lectin recognition and sialidase-mediated hydrolysis.
30063825	6	9	theme	glycan-involved	1236:1250	arg1	hydrolysis					1324:1333	sialidase-mediated hydrolysis	1305:1333	sialidase-mediated hydrolysis	1305:1333	Our findings support the potential use of such fluorescent platforms to sense glycan-involved biological processes, such as lectin recognition and sialidase-mediated hydrolysis.
30063825	4	10	theme	sialic	1029:1034	arg1	moiety					1041:1046	a sialic acid moiety	1027:1046	a sialic acid moiety	1027:1046	Here we provide two distinct methodologies, covalent bioconjugation and self-assembly, to functionalize two types of Quantum Dots with a homogeneous, complex-type N-linked glycan terminated with a sialic acid moiety.
30063825	4	11	theme	N-linked	995:1002	arg1	glycan					1004:1009	a homogeneous, complex-type N-linked glycan	967:1009	a homogeneous, complex-type N-linked glycan terminated with a sialic acid moiety	967:1046	Here we provide two distinct methodologies, covalent bioconjugation and self-assembly, to functionalize two types of Quantum Dots with a homogeneous, complex-type N-linked glycan terminated with a sialic acid moiety.
30063825	1	12	theme	several	381:387	arg1	groups					389:394	several groups	381:394	several groups	381:394	Coating inorganic nanoparticles with polyethylene glycol (PEG)-appended ligands, as means to preserve their physical characteristics and promote steric interactions with biological systems, including enhanced aqueous solubility and reduced immunogenicity, has been explored by several groups.
30063825	6	13	theme	sense	1230:1234	arg1	recognition					1289:1299	lectin recognition	1282:1299	lectin recognition	1282:1299	Our findings support the potential use of such fluorescent platforms to sense glycan-involved biological processes, such as lectin recognition and sialidase-mediated hydrolysis.
30063825	6	13	theme	sense	1230:1234	arg1	processes					1263:1271	sense glycan-involved biological processes	1230:1271	sense glycan-involved biological processes	1230:1271	Our findings support the potential use of such fluorescent platforms to sense glycan-involved biological processes, such as lectin recognition and sialidase-mediated hydrolysis.
30063825	6	13	theme	sense	1230:1234	arg1	hydrolysis					1324:1333	sialidase-mediated hydrolysis	1305:1333	sialidase-mediated hydrolysis	1305:1333	Our findings support the potential use of such fluorescent platforms to sense glycan-involved biological processes, such as lectin recognition and sialidase-mediated hydrolysis.
30063825	2	14	theme	present	424:430	arg1	macromolecules					409:422	macromolecules	409:422	macromolecules present in the human serum and on the surface of cells	409:477	Conversely, macromolecules present in the human serum and on the surface of cells are densely coated with hydrophilic glycans that act to reduce nonspecific interactions, while facilitating specific binding and interactions.
30063825	3	15	link	N-linked	637:644	arg1	glycans					646:652	N-linked glycans	637:652	N-linked glycans	637:652	In particular, N-linked glycans are abundant on the surface of most serum proteins and are composed of a branched architecture that is typically characterized by a significant level of molecular heterogeneity.
30063825	3	16	theme	branched	727:734	arg1	architecture					736:747	a branched architecture	725:747	a branched architecture that is typically characterized by a significant level of molecular heterogeneity	725:829	In particular, N-linked glycans are abundant on the surface of most serum proteins and are composed of a branched architecture that is typically characterized by a significant level of molecular heterogeneity.
30063825	1	17	with	nanoparticles	122:134	arg1	ligands					176:182	polyethylene glycol (PEG)-appended ligands	141:182	polyethylene glycol (PEG)-appended ligands	141:182	Coating inorganic nanoparticles with polyethylene glycol (PEG)-appended ligands, as means to preserve their physical characteristics and promote steric interactions with biological systems, including enhanced aqueous solubility and reduced immunogenicity, has been explored by several groups.
30063825	6	18	theme	platforms	1217:1225	arg1	use					1193:1195	the potential use	1179:1195	the potential use of such fluorescent platforms to sense glycan-involved biological processes, such as lectin recognition and sialidase-mediated hydrolysis	1179:1333	Our findings support the potential use of such fluorescent platforms to sense glycan-involved biological processes, such as lectin recognition and sialidase-mediated hydrolysis.
30063825	0	19	theme	Efficient	0:8	arg1	Assembly					10:17	Efficient Assembly	0:17	Efficient Assembly of Quantum Dots with Homogenous Glycans	0:57	Efficient Assembly of Quantum Dots with Homogenous Glycans Derived from Natural N-Linked Glycoproteins.
30063825	4	20	theme	covalent	876:883	arg1	methodologies					861:873	two distinct methodologies	848:873	two distinct methodologies	848:873	Here we provide two distinct methodologies, covalent bioconjugation and self-assembly, to functionalize two types of Quantum Dots with a homogeneous, complex-type N-linked glycan terminated with a sialic acid moiety.
30063825	4	20	theme	covalent	876:883	arg1	bioconjugation					885:898	covalent bioconjugation	876:898	covalent bioconjugation	876:898	Here we provide two distinct methodologies, covalent bioconjugation and self-assembly, to functionalize two types of Quantum Dots with a homogeneous, complex-type N-linked glycan terminated with a sialic acid moiety.
30063825	5	21	theme	functional	1073:1082	arg1	characterization					1084:1099	A detailed physical and functional characterization	1049:1099	A detailed physical and functional characterization of these glycan-coated nanoparticles	1049:1136	A detailed physical and functional characterization of these glycan-coated nanoparticles has been performed.
30063825	0	22	theme	Quantum	22:28	arg1	Dots					30:33	Quantum Dots	22:33	Quantum Dots	22:33	Efficient Assembly of Quantum Dots with Homogenous Glycans Derived from Natural N-Linked Glycoproteins.
30063825	4	23	theme	Dots	957:960	arg1	types					940:944	two types	936:944	two types of Quantum Dots	936:960	Here we provide two distinct methodologies, covalent bioconjugation and self-assembly, to functionalize two types of Quantum Dots with a homogeneous, complex-type N-linked glycan terminated with a sialic acid moiety.
30063825	6	24	theme	lectin	1282:1287	arg1	recognition					1289:1299	lectin recognition	1282:1299	lectin recognition	1282:1299	Our findings support the potential use of such fluorescent platforms to sense glycan-involved biological processes, such as lectin recognition and sialidase-mediated hydrolysis.
30063825	1	25	theme	Coating	104:110	arg1	nanoparticles					122:134	Coating inorganic nanoparticles	104:134	Coating inorganic nanoparticles	104:134	Coating inorganic nanoparticles with polyethylene glycol (PEG)-appended ligands, as means to preserve their physical characteristics and promote steric interactions with biological systems, including enhanced aqueous solubility and reduced immunogenicity, has been explored by several groups.
30063825	1	25	theme	Coating	104:110	arg1	solubility					321:330	enhanced aqueous solubility	304:330	enhanced aqueous solubility	304:330	Coating inorganic nanoparticles with polyethylene glycol (PEG)-appended ligands, as means to preserve their physical characteristics and promote steric interactions with biological systems, including enhanced aqueous solubility and reduced immunogenicity, has been explored by several groups.
30063825	1	25	theme	Coating	104:110	arg1	immunogenicity					344:357	reduced immunogenicity	336:357	reduced immunogenicity	336:357	Coating inorganic nanoparticles with polyethylene glycol (PEG)-appended ligands, as means to preserve their physical characteristics and promote steric interactions with biological systems, including enhanced aqueous solubility and reduced immunogenicity, has been explored by several groups.
30063825	6	26	theme	fluorescent	1205:1215	arg1	platforms					1217:1225	such fluorescent platforms	1200:1225	such fluorescent platforms	1200:1225	Our findings support the potential use of such fluorescent platforms to sense glycan-involved biological processes, such as lectin recognition and sialidase-mediated hydrolysis.
30063825	1	27	theme	enhanced	304:311	arg1	solubility					321:330	enhanced aqueous solubility	304:330	enhanced aqueous solubility	304:330	Coating inorganic nanoparticles with polyethylene glycol (PEG)-appended ligands, as means to preserve their physical characteristics and promote steric interactions with biological systems, including enhanced aqueous solubility and reduced immunogenicity, has been explored by several groups.
30063825	1	28	with	interactions	256:267	arg1	systems					285:291	biological systems	274:291	biological systems	274:291	Coating inorganic nanoparticles with polyethylene glycol (PEG)-appended ligands, as means to preserve their physical characteristics and promote steric interactions with biological systems, including enhanced aqueous solubility and reduced immunogenicity, has been explored by several groups.
30063825	5	29	theme	glycan-coated	1110:1122	arg1	nanoparticles					1124:1136	these glycan-coated nanoparticles	1104:1136	these glycan-coated nanoparticles	1104:1136	A detailed physical and functional characterization of these glycan-coated nanoparticles has been performed.
30063825	1	30	theme	inorganic	112:120	arg1	nanoparticles					122:134	Coating inorganic nanoparticles	104:134	Coating inorganic nanoparticles	104:134	Coating inorganic nanoparticles with polyethylene glycol (PEG)-appended ligands, as means to preserve their physical characteristics and promote steric interactions with biological systems, including enhanced aqueous solubility and reduced immunogenicity, has been explored by several groups.
30063825	1	30	theme	inorganic	112:120	arg1	solubility					321:330	enhanced aqueous solubility	304:330	enhanced aqueous solubility	304:330	Coating inorganic nanoparticles with polyethylene glycol (PEG)-appended ligands, as means to preserve their physical characteristics and promote steric interactions with biological systems, including enhanced aqueous solubility and reduced immunogenicity, has been explored by several groups.
30063825	1	30	theme	inorganic	112:120	arg1	immunogenicity					344:357	reduced immunogenicity	336:357	reduced immunogenicity	336:357	Coating inorganic nanoparticles with polyethylene glycol (PEG)-appended ligands, as means to preserve their physical characteristics and promote steric interactions with biological systems, including enhanced aqueous solubility and reduced immunogenicity, has been explored by several groups.
30063825	2	31	theme	human	439:443	arg1	serum					445:449	the human serum	435:449	the human serum	435:449	Conversely, macromolecules present in the human serum and on the surface of cells are densely coated with hydrophilic glycans that act to reduce nonspecific interactions, while facilitating specific binding and interactions.
30063825	4	32	theme	acid	1036:1039	arg1	moiety					1041:1046	a sialic acid moiety	1027:1046	a sialic acid moiety	1027:1046	Here we provide two distinct methodologies, covalent bioconjugation and self-assembly, to functionalize two types of Quantum Dots with a homogeneous, complex-type N-linked glycan terminated with a sialic acid moiety.
30063825	1	33	theme	aqueous	313:319	arg1	solubility					321:330	enhanced aqueous solubility	304:330	enhanced aqueous solubility	304:330	Coating inorganic nanoparticles with polyethylene glycol (PEG)-appended ligands, as means to preserve their physical characteristics and promote steric interactions with biological systems, including enhanced aqueous solubility and reduced immunogenicity, has been explored by several groups.
30063825	5	34	theme	nanoparticles	1124:1136	arg1	characterization					1084:1099	A detailed physical and functional characterization	1049:1099	A detailed physical and functional characterization of these glycan-coated nanoparticles	1049:1136	A detailed physical and functional characterization of these glycan-coated nanoparticles has been performed.
30063825	6	35	theme	sialidase-mediated	1305:1322	arg1	hydrolysis					1324:1333	sialidase-mediated hydrolysis	1305:1333	sialidase-mediated hydrolysis	1305:1333	Our findings support the potential use of such fluorescent platforms to sense glycan-involved biological processes, such as lectin recognition and sialidase-mediated hydrolysis.
30063825	3	36	from	particular	625:634	arg1	abundant					658:665	abundant	658:665	abundant	658:665	In particular, N-linked glycans are abundant on the surface of most serum proteins and are composed of a branched architecture that is typically characterized by a significant level of molecular heterogeneity.
30063825	0	37	theme	Dots	30:33	arg1	Assembly					10:17	Efficient Assembly	0:17	Efficient Assembly of Quantum Dots with Homogenous Glycans	0:57	Efficient Assembly of Quantum Dots with Homogenous Glycans Derived from Natural N-Linked Glycoproteins.
30063825	2	38	theme	nonspecific	542:552	arg1	interactions					554:565	nonspecific interactions	542:565	nonspecific interactions	542:565	Conversely, macromolecules present in the human serum and on the surface of cells are densely coated with hydrophilic glycans that act to reduce nonspecific interactions, while facilitating specific binding and interactions.
30063825	5	39	theme	detailed	1051:1058	arg1	characterization					1084:1099	A detailed physical and functional characterization	1049:1099	A detailed physical and functional characterization of these glycan-coated nanoparticles	1049:1136	A detailed physical and functional characterization of these glycan-coated nanoparticles has been performed.
30063825	1	40	theme	physical	212:219	arg1	characteristics					221:235	their physical characteristics	206:235	their physical characteristics	206:235	Coating inorganic nanoparticles with polyethylene glycol (PEG)-appended ligands, as means to preserve their physical characteristics and promote steric interactions with biological systems, including enhanced aqueous solubility and reduced immunogenicity, has been explored by several groups.
30063825	2	41	theme	cells	473:477	arg1	surface					462:468	the surface	458:468	the surface of cells	458:477	Conversely, macromolecules present in the human serum and on the surface of cells are densely coated with hydrophilic glycans that act to reduce nonspecific interactions, while facilitating specific binding and interactions.
30063825	4	42	link	N-linked	995:1002	arg1	glycan					1004:1009	a homogeneous, complex-type N-linked glycan	967:1009	a homogeneous, complex-type N-linked glycan terminated with a sialic acid moiety	967:1046	Here we provide two distinct methodologies, covalent bioconjugation and self-assembly, to functionalize two types of Quantum Dots with a homogeneous, complex-type N-linked glycan terminated with a sialic acid moiety.
30063825	4	43	theme	distinct	852:859	arg1	self-assembly					904:916	self-assembly	904:916	self-assembly	904:916	Here we provide two distinct methodologies, covalent bioconjugation and self-assembly, to functionalize two types of Quantum Dots with a homogeneous, complex-type N-linked glycan terminated with a sialic acid moiety.
30063825	4	43	theme	distinct	852:859	arg1	methodologies					861:873	two distinct methodologies	848:873	two distinct methodologies	848:873	Here we provide two distinct methodologies, covalent bioconjugation and self-assembly, to functionalize two types of Quantum Dots with a homogeneous, complex-type N-linked glycan terminated with a sialic acid moiety.
30063825	4	43	theme	distinct	852:859	arg1	bioconjugation					885:898	covalent bioconjugation	876:898	covalent bioconjugation	876:898	Here we provide two distinct methodologies, covalent bioconjugation and self-assembly, to functionalize two types of Quantum Dots with a homogeneous, complex-type N-linked glycan terminated with a sialic acid moiety.
30063825	0	44	theme	Homogenous	40:49	arg1	Glycans					51:57	Homogenous Glycans	40:57	Homogenous Glycans	40:57	Efficient Assembly of Quantum Dots with Homogenous Glycans Derived from Natural N-Linked Glycoproteins.
30063825	1	45	theme	reduced	336:342	arg1	immunogenicity					344:357	reduced immunogenicity	336:357	reduced immunogenicity	336:357	Coating inorganic nanoparticles with polyethylene glycol (PEG)-appended ligands, as means to preserve their physical characteristics and promote steric interactions with biological systems, including enhanced aqueous solubility and reduced immunogenicity, has been explored by several groups.
30063825	3	46	theme	serum	690:694	arg1	proteins					696:703	most serum proteins	685:703	most serum proteins	685:703	In particular, N-linked glycans are abundant on the surface of most serum proteins and are composed of a branched architecture that is typically characterized by a significant level of molecular heterogeneity.
30063825	3	47	theme	proteins	696:703	arg1	surface					674:680	the surface	670:680	the surface of most serum proteins	670:703	In particular, N-linked glycans are abundant on the surface of most serum proteins and are composed of a branched architecture that is typically characterized by a significant level of molecular heterogeneity.
30063825	0	48	with	Assembly	10:17	arg1	Glycans					51:57	Homogenous Glycans	40:57	Homogenous Glycans	40:57	Efficient Assembly of Quantum Dots with Homogenous Glycans Derived from Natural N-Linked Glycoproteins.
30063825	6	49	theme	potential	1183:1191	arg1	use					1193:1195	the potential use	1179:1195	the potential use of such fluorescent platforms to sense glycan-involved biological processes, such as lectin recognition and sialidase-mediated hydrolysis	1179:1333	Our findings support the potential use of such fluorescent platforms to sense glycan-involved biological processes, such as lectin recognition and sialidase-mediated hydrolysis.
30063825	3	50	theme	N-linked	637:644	arg1	glycans					646:652	N-linked glycans	637:652	N-linked glycans	637:652	In particular, N-linked glycans are abundant on the surface of most serum proteins and are composed of a branched architecture that is typically characterized by a significant level of molecular heterogeneity.
30063825	2	51	theme	specific	587:594	arg1	binding					596:602	specific binding	587:602	specific binding	587:602	Conversely, macromolecules present in the human serum and on the surface of cells are densely coated with hydrophilic glycans that act to reduce nonspecific interactions, while facilitating specific binding and interactions.
30063825	2	52	attach	present	424:430	arg1	surface					462:468	the surface	458:468	the surface of cells	458:477	Conversely, macromolecules present in the human serum and on the surface of cells are densely coated with hydrophilic glycans that act to reduce nonspecific interactions, while facilitating specific binding and interactions.
30063825	2	52	attach	present	424:430	arg1	serum					445:449	the human serum	435:449	the human serum	435:449	Conversely, macromolecules present in the human serum and on the surface of cells are densely coated with hydrophilic glycans that act to reduce nonspecific interactions, while facilitating specific binding and interactions.
30063825	2	52	attach	present	424:430	arg2	macromolecules					409:422	macromolecules	409:422	macromolecules present in the human serum and on the surface of cells	409:477	Conversely, macromolecules present in the human serum and on the surface of cells are densely coated with hydrophilic glycans that act to reduce nonspecific interactions, while facilitating specific binding and interactions.
30063825	1	53	theme	polyethylene	141:152	arg1	PEG					162:164	PEG	162:164	PEG	162:164	Coating inorganic nanoparticles with polyethylene glycol (PEG)-appended ligands, as means to preserve their physical characteristics and promote steric interactions with biological systems, including enhanced aqueous solubility and reduced immunogenicity, has been explored by several groups.
30063825	1	53	theme	polyethylene	141:152	arg1	glycol					154:159	polyethylene glycol	141:159	polyethylene glycol (PEG)-appended ligands	141:182	Coating inorganic nanoparticles with polyethylene glycol (PEG)-appended ligands, as means to preserve their physical characteristics and promote steric interactions with biological systems, including enhanced aqueous solubility and reduced immunogenicity, has been explored by several groups.
30063825	6	54	theme	such	1200:1203	arg1	platforms					1217:1225	such fluorescent platforms	1200:1225	such fluorescent platforms	1200:1225	Our findings support the potential use of such fluorescent platforms to sense glycan-involved biological processes, such as lectin recognition and sialidase-mediated hydrolysis.
30063825	3	55	theme	significant	786:796	arg1	level					798:802	a significant level	784:802	a significant level of molecular heterogeneity	784:829	In particular, N-linked glycans are abundant on the surface of most serum proteins and are composed of a branched architecture that is typically characterized by a significant level of molecular heterogeneity.
30063825	1	56	theme	glycol	154:159	arg1	ligands					176:182	polyethylene glycol (PEG)-appended ligands	141:182	polyethylene glycol (PEG)-appended ligands	141:182	Coating inorganic nanoparticles with polyethylene glycol (PEG)-appended ligands, as means to preserve their physical characteristics and promote steric interactions with biological systems, including enhanced aqueous solubility and reduced immunogenicity, has been explored by several groups.
30063825	4	57	theme	Quantum	949:955	arg1	Dots					957:960	Quantum Dots	949:960	Quantum Dots	949:960	Here we provide two distinct methodologies, covalent bioconjugation and self-assembly, to functionalize two types of Quantum Dots with a homogeneous, complex-type N-linked glycan terminated with a sialic acid moiety.
30063825	3	58	theme	most	685:688	arg1	proteins					696:703	most serum proteins	685:703	most serum proteins	685:703	In particular, N-linked glycans are abundant on the surface of most serum proteins and are composed of a branched architecture that is typically characterized by a significant level of molecular heterogeneity.
30063825	3	59	theme	molecular	807:815	arg1	heterogeneity					817:829	molecular heterogeneity	807:829	molecular heterogeneity	807:829	In particular, N-linked glycans are abundant on the surface of most serum proteins and are composed of a branched architecture that is typically characterized by a significant level of molecular heterogeneity.
30063825	2	60	theme	hydrophilic	503:513	arg1	glycans					515:521	hydrophilic glycans	503:521	hydrophilic glycans that act to reduce nonspecific interactions, while facilitating specific binding and interactions	503:619	Conversely, macromolecules present in the human serum and on the surface of cells are densely coated with hydrophilic glycans that act to reduce nonspecific interactions, while facilitating specific binding and interactions.
30063825	3	61	theme	heterogeneity	817:829	arg1	level					798:802	a significant level	784:802	a significant level of molecular heterogeneity	784:829	In particular, N-linked glycans are abundant on the surface of most serum proteins and are composed of a branched architecture that is typically characterized by a significant level of molecular heterogeneity.
30063825	2	62	from	serum	445:449	arg1	present					424:430	present	424:430	present	424:430	Conversely, macromolecules present in the human serum and on the surface of cells are densely coated with hydrophilic glycans that act to reduce nonspecific interactions, while facilitating specific binding and interactions.
30063825	2	63	from	present	424:430	arg1	serum					445:449	the human serum	435:449	the human serum	435:449	Conversely, macromolecules present in the human serum and on the surface of cells are densely coated with hydrophilic glycans that act to reduce nonspecific interactions, while facilitating specific binding and interactions.
30063825	1	64	theme	steric	249:254	arg1	interactions					256:267	steric interactions	249:267	steric interactions with biological systems	249:291	Coating inorganic nanoparticles with polyethylene glycol (PEG)-appended ligands, as means to preserve their physical characteristics and promote steric interactions with biological systems, including enhanced aqueous solubility and reduced immunogenicity, has been explored by several groups.
30740837	5	0	theme	Con	767:769	arg1	interference					773:784	the Con A interference	763:784	the Con A interference with the pmTSP-II-induced acrosome reaction (AR)	763:833	The function of the Man glycoconjugates was evident by the Con A interference with the pmTSP-II-induced acrosome reaction (AR) as well as by the ability to recover the induction of the AR by the inclusion of Mans in the treatment mixture.
30740837	0	1	from	glycoconjugates	17:31	arg1	thrombospondin					43:56	shrimp thrombospondin	36:56	shrimp thrombospondin	36:56	N-linked mannose glycoconjugates on shrimp thrombospondin, pmTSP-II, and their involvement in the sperm acrosome reaction.
30740837	0	1	from	glycoconjugates	17:31	arg1	involvement					79:89	their involvement	73:89	their involvement in the sperm acrosome reaction	73:120	N-linked mannose glycoconjugates on shrimp thrombospondin, pmTSP-II, and their involvement in the sperm acrosome reaction.
30740837	0	1	from	glycoconjugates	17:31	arg1	pmTSP-II					59:66	pmTSP-II	59:66	pmTSP-II	59:66	N-linked mannose glycoconjugates on shrimp thrombospondin, pmTSP-II, and their involvement in the sperm acrosome reaction.
30740837	5	2	theme	A	771:771	arg1	interference					773:784	the Con A interference	763:784	the Con A interference with the pmTSP-II-induced acrosome reaction (AR)	763:833	The function of the Man glycoconjugates was evident by the Con A interference with the pmTSP-II-induced acrosome reaction (AR) as well as by the ability to recover the induction of the AR by the inclusion of Mans in the treatment mixture.
30740837	6	3	theme	domains	1017:1023	arg1	proteins					976:983	the recombinant proteins	960:983	the recombinant proteins of the three signature pmTSP-II domains expressed in E. coli (lacking glycosylation) and mannosidase-treated pmTSP-II	960:1101	In addition, the recombinant proteins of the three signature pmTSP-II domains expressed in E. coli (lacking glycosylation) and mannosidase-treated pmTSP-II showed a minimal ability to initiate the AR response.
30740837	2	4	theme	physiological	341:353	arg1	functions					355:363	their physiological functions	335:363	their physiological functions	335:363	Here, the presence of N-linked mannose (Man) glycoconjugates on shrimp thrombospondin ( pmTSP-II) and their physiological functions were investigated in the black tiger shrimp Penaeus monodon.
30740837	5	5	theme	Man	728:730	arg1	glycoconjugates					732:746	the Man glycoconjugates	724:746	the Man glycoconjugates	724:746	The function of the Man glycoconjugates was evident by the Con A interference with the pmTSP-II-induced acrosome reaction (AR) as well as by the ability to recover the induction of the AR by the inclusion of Mans in the treatment mixture.
30740837	7	6	theme	TSP	1303:1305	arg1	protein					1314:1320	a TSP family protein	1301:1320	a TSP family protein	1301:1320	Together, these results provide evidence of the pivotal role that Man-linked pmTSP-II plays in modulating the shrimp sperm AR, a novel role for a TSP family protein in shrimp reproductive biology.
30740837	4	7	theme	protease	687:694	arg1	inhibitor					696:704	protease inhibitor	687:704	protease inhibitor	687:704	The presence of Man residues was verified by concanavalin A lectin histochemistry on the purified fraction of pmTSP-II (250 kDa with protease inhibitor).
30740837	2	8	theme	glycoconjugates	278:292	arg1	presence					243:250	the presence	239:250	the presence of N-linked mannose (Man) glycoconjugates on shrimp thrombospondin ( pmTSP-II) and their physiological functions	239:363	Here, the presence of N-linked mannose (Man) glycoconjugates on shrimp thrombospondin ( pmTSP-II) and their physiological functions were investigated in the black tiger shrimp Penaeus monodon.
30740837	6	9	theme	signature	998:1006	arg1	domains					1017:1023	the three signature pmTSP-II domains	988:1023	the three signature pmTSP-II domains expressed in E. coli (lacking glycosylation) and mannosidase-treated pmTSP-II	988:1101	In addition, the recombinant proteins of the three signature pmTSP-II domains expressed in E. coli (lacking glycosylation) and mannosidase-treated pmTSP-II showed a minimal ability to initiate the AR response.
30740837	4	10	theme	concanavalin	599:610	arg1	A					612:612	concanavalin A	599:612	concanavalin A lectin histochemistry	599:634	The presence of Man residues was verified by concanavalin A lectin histochemistry on the purified fraction of pmTSP-II (250 kDa with protease inhibitor).
30740837	5	11	theme	glycoconjugates	732:746	arg1	function					712:719	The function	708:719	The function of the Man glycoconjugates	708:746	The function of the Man glycoconjugates was evident by the Con A interference with the pmTSP-II-induced acrosome reaction (AR) as well as by the ability to recover the induction of the AR by the inclusion of Mans in the treatment mixture.
30740837	5	11	theme	glycoconjugates	732:746	arg1	evident					752:758	evident	752:758	evident	752:758	The function of the Man glycoconjugates was evident by the Con A interference with the pmTSP-II-induced acrosome reaction (AR) as well as by the ability to recover the induction of the AR by the inclusion of Mans in the treatment mixture.
30740837	0	12	from	pmTSP-II	59:66	arg1	reaction					113:120	the sperm acrosome reaction	94:120	the sperm acrosome reaction	94:120	N-linked mannose glycoconjugates on shrimp thrombospondin, pmTSP-II, and their involvement in the sperm acrosome reaction.
30740837	6	13	theme	AR	1144:1145	arg1	response					1147:1154	the AR response	1140:1154	the AR response	1140:1154	In addition, the recombinant proteins of the three signature pmTSP-II domains expressed in E. coli (lacking glycosylation) and mannosidase-treated pmTSP-II showed a minimal ability to initiate the AR response.
30740837	5	14	theme	treatment	928:936	arg1	mixture					938:944	the treatment mixture	924:944	the treatment mixture	924:944	The function of the Man glycoconjugates was evident by the Con A interference with the pmTSP-II-induced acrosome reaction (AR) as well as by the ability to recover the induction of the AR by the inclusion of Mans in the treatment mixture.
30740837	7	15	from	role	1292:1295	arg1	biology					1345:1351	shrimp reproductive biology	1325:1351	shrimp reproductive biology	1325:1351	Together, these results provide evidence of the pivotal role that Man-linked pmTSP-II plays in modulating the shrimp sperm AR, a novel role for a TSP family protein in shrimp reproductive biology.
30740837	2	16	link	N-linked	255:262	arg1	Man					273:275	Man	273:275	Man	273:275	Here, the presence of N-linked mannose (Man) glycoconjugates on shrimp thrombospondin ( pmTSP-II) and their physiological functions were investigated in the black tiger shrimp Penaeus monodon.
30740837	2	16	link	N-linked	255:262	arg1	mannose					264:270	N-linked mannose	255:270	N-linked mannose (Man) glycoconjugates	255:292	Here, the presence of N-linked mannose (Man) glycoconjugates on shrimp thrombospondin ( pmTSP-II) and their physiological functions were investigated in the black tiger shrimp Penaeus monodon.
30740837	0	17	theme	acrosome	104:111	arg1	reaction					113:120	the sperm acrosome reaction	94:120	the sperm acrosome reaction	94:120	N-linked mannose glycoconjugates on shrimp thrombospondin, pmTSP-II, and their involvement in the sperm acrosome reaction.
30740837	7	18	theme	role	1213:1216	arg1	evidence					1189:1196	evidence	1189:1196	evidence of the pivotal role that Man-linked pmTSP-II plays in modulating the shrimp sperm AR, a novel role for a TSP family protein in shrimp reproductive biology	1189:1351	Together, these results provide evidence of the pivotal role that Man-linked pmTSP-II plays in modulating the shrimp sperm AR, a novel role for a TSP family protein in shrimp reproductive biology.
30740837	0	19	link	N-linked	0:7	arg1	glycoconjugates					17:31	N-linked mannose glycoconjugates	0:31	N-linked mannose glycoconjugates on shrimp thrombospondin, pmTSP-II, and their involvement in the sperm acrosome reaction	0:120	N-linked mannose glycoconjugates on shrimp thrombospondin, pmTSP-II, and their involvement in the sperm acrosome reaction.
30740837	3	20	theme	TSP3	540:543	arg1	domains					545:551	TSP3 domains	540:551	TSP3 domains	540:551	A molecular analysis of pmTSP-II demonstrated anchorage sites for N-linked glycans in both the chitin-binding and TSP3 domains.
30740837	2	21	theme	tiger	396:400	arg1	monodon					417:423	the black tiger shrimp Penaeus monodon	386:423	the black tiger shrimp Penaeus monodon	386:423	Here, the presence of N-linked mannose (Man) glycoconjugates on shrimp thrombospondin ( pmTSP-II) and their physiological functions were investigated in the black tiger shrimp Penaeus monodon.
30740837	0	22	theme	sperm	98:102	arg1	reaction					113:120	the sperm acrosome reaction	94:120	the sperm acrosome reaction	94:120	N-linked mannose glycoconjugates on shrimp thrombospondin, pmTSP-II, and their involvement in the sperm acrosome reaction.
30740837	0	23	from	thrombospondin	43:56	arg1	reaction					113:120	the sperm acrosome reaction	94:120	the sperm acrosome reaction	94:120	N-linked mannose glycoconjugates on shrimp thrombospondin, pmTSP-II, and their involvement in the sperm acrosome reaction.
30740837	5	24	theme	acrosome	812:819	arg1	reaction					821:828	the pmTSP-II-induced acrosome reaction	791:828	the pmTSP-II-induced acrosome reaction (AR)	791:833	The function of the Man glycoconjugates was evident by the Con A interference with the pmTSP-II-induced acrosome reaction (AR) as well as by the ability to recover the induction of the AR by the inclusion of Mans in the treatment mixture.
30740837	5	24	theme	acrosome	812:819	arg1	AR					831:832	AR	831:832	AR	831:832	The function of the Man glycoconjugates was evident by the Con A interference with the pmTSP-II-induced acrosome reaction (AR) as well as by the ability to recover the induction of the AR by the inclusion of Mans in the treatment mixture.
30740837	1	25	from	Glycoconjugates	123:137	arg1	matrices					160:167	egg extracellular matrices	142:167	egg extracellular matrices	142:167	Glycoconjugates in egg extracellular matrices are known to serve several functions in reproductive processes.
30740837	2	26	theme	black	390:394	arg1	monodon					417:423	the black tiger shrimp Penaeus monodon	386:423	the black tiger shrimp Penaeus monodon	386:423	Here, the presence of N-linked mannose (Man) glycoconjugates on shrimp thrombospondin ( pmTSP-II) and their physiological functions were investigated in the black tiger shrimp Penaeus monodon.
30740837	0	27	theme	mannose	9:15	arg1	glycoconjugates					17:31	N-linked mannose glycoconjugates	0:31	N-linked mannose glycoconjugates on shrimp thrombospondin, pmTSP-II, and their involvement in the sperm acrosome reaction	0:120	N-linked mannose glycoconjugates on shrimp thrombospondin, pmTSP-II, and their involvement in the sperm acrosome reaction.
30740837	7	28	dep	role	1213:1216	arg1	plays					1243:1247	plays	1243:1247	plays	1243:1247	Together, these results provide evidence of the pivotal role that Man-linked pmTSP-II plays in modulating the shrimp sperm AR, a novel role for a TSP family protein in shrimp reproductive biology.
30740837	0	29	theme	N-linked	0:7	arg1	glycoconjugates					17:31	N-linked mannose glycoconjugates	0:31	N-linked mannose glycoconjugates on shrimp thrombospondin, pmTSP-II, and their involvement in the sperm acrosome reaction	0:120	N-linked mannose glycoconjugates on shrimp thrombospondin, pmTSP-II, and their involvement in the sperm acrosome reaction.
30740837	7	30	theme	novel	1286:1290	arg1	role					1292:1295	a novel role	1284:1295	a novel role for a TSP family protein in shrimp reproductive biology	1284:1351	Together, these results provide evidence of the pivotal role that Man-linked pmTSP-II plays in modulating the shrimp sperm AR, a novel role for a TSP family protein in shrimp reproductive biology.
30740837	1	31	theme	several	188:194	arg1	functions					196:204	several functions	188:204	several functions in reproductive processes	188:230	Glycoconjugates in egg extracellular matrices are known to serve several functions in reproductive processes.
30740837	2	32	theme	shrimp	297:302	arg1	pmTSP-II					321:328	pmTSP-II	321:328	pmTSP-II	321:328	Here, the presence of N-linked mannose (Man) glycoconjugates on shrimp thrombospondin ( pmTSP-II) and their physiological functions were investigated in the black tiger shrimp Penaeus monodon.
30740837	2	32	theme	shrimp	297:302	arg1	thrombospondin					304:317	shrimp thrombospondin	297:317	shrimp thrombospondin ( pmTSP-II)	297:329	Here, the presence of N-linked mannose (Man) glycoconjugates on shrimp thrombospondin ( pmTSP-II) and their physiological functions were investigated in the black tiger shrimp Penaeus monodon.
30740837	2	33	from	presence	243:250	arg1	functions					355:363	their physiological functions	335:363	their physiological functions	335:363	Here, the presence of N-linked mannose (Man) glycoconjugates on shrimp thrombospondin ( pmTSP-II) and their physiological functions were investigated in the black tiger shrimp Penaeus monodon.
30740837	2	33	from	presence	243:250	arg1	pmTSP-II					321:328	pmTSP-II	321:328	pmTSP-II	321:328	Here, the presence of N-linked mannose (Man) glycoconjugates on shrimp thrombospondin ( pmTSP-II) and their physiological functions were investigated in the black tiger shrimp Penaeus monodon.
30740837	2	33	from	presence	243:250	arg1	thrombospondin					304:317	shrimp thrombospondin	297:317	shrimp thrombospondin ( pmTSP-II)	297:329	Here, the presence of N-linked mannose (Man) glycoconjugates on shrimp thrombospondin ( pmTSP-II) and their physiological functions were investigated in the black tiger shrimp Penaeus monodon.
30740837	3	34	theme	anchorage	472:480	arg1	sites					482:486	anchorage sites	472:486	anchorage sites for N-linked glycans in both the chitin-binding and TSP3 domains	472:551	A molecular analysis of pmTSP-II demonstrated anchorage sites for N-linked glycans in both the chitin-binding and TSP3 domains.
30740837	4	35	theme	residues	574:581	arg1	presence					558:565	The presence	554:565	The presence of Man residues	554:581	The presence of Man residues was verified by concanavalin A lectin histochemistry on the purified fraction of pmTSP-II (250 kDa with protease inhibitor).
30740837	5	36	with	interference	773:784	arg1	reaction					821:828	the pmTSP-II-induced acrosome reaction	791:828	the pmTSP-II-induced acrosome reaction (AR)	791:833	The function of the Man glycoconjugates was evident by the Con A interference with the pmTSP-II-induced acrosome reaction (AR) as well as by the ability to recover the induction of the AR by the inclusion of Mans in the treatment mixture.
30740837	5	36	with	interference	773:784	arg1	AR					831:832	AR	831:832	AR	831:832	The function of the Man glycoconjugates was evident by the Con A interference with the pmTSP-II-induced acrosome reaction (AR) as well as by the ability to recover the induction of the AR by the inclusion of Mans in the treatment mixture.
30740837	0	37	from	involvement	79:89	arg1	reaction					113:120	the sperm acrosome reaction	94:120	the sperm acrosome reaction	94:120	N-linked mannose glycoconjugates on shrimp thrombospondin, pmTSP-II, and their involvement in the sperm acrosome reaction.
30740837	7	38	theme	pivotal	1205:1211	arg1	role					1213:1216	the pivotal role	1201:1216	the pivotal role that Man-linked pmTSP-II plays in modulating the shrimp sperm AR, a novel role for a TSP family protein in shrimp reproductive biology	1201:1351	Together, these results provide evidence of the pivotal role that Man-linked pmTSP-II plays in modulating the shrimp sperm AR, a novel role for a TSP family protein in shrimp reproductive biology.
30740837	6	39	theme	pmTSP-II	1008:1015	arg1	domains					1017:1023	the three signature pmTSP-II domains	988:1023	the three signature pmTSP-II domains expressed in E. coli (lacking glycosylation) and mannosidase-treated pmTSP-II	988:1101	In addition, the recombinant proteins of the three signature pmTSP-II domains expressed in E. coli (lacking glycosylation) and mannosidase-treated pmTSP-II showed a minimal ability to initiate the AR response.
30740837	7	40	theme	shrimp	1267:1272	arg1	AR					1280:1281	the shrimp sperm AR	1263:1281	the shrimp sperm AR	1263:1281	Together, these results provide evidence of the pivotal role that Man-linked pmTSP-II plays in modulating the shrimp sperm AR, a novel role for a TSP family protein in shrimp reproductive biology.
30740837	4	41	theme	Man	570:572	arg1	residues					574:581	Man residues	570:581	Man residues	570:581	The presence of Man residues was verified by concanavalin A lectin histochemistry on the purified fraction of pmTSP-II (250 kDa with protease inhibitor).
30740837	1	42	theme	reproductive	209:220	arg1	processes					222:230	reproductive processes	209:230	reproductive processes	209:230	Glycoconjugates in egg extracellular matrices are known to serve several functions in reproductive processes.
30740837	3	43	theme	molecular	428:436	arg1	analysis					438:445	A molecular analysis	426:445	A molecular analysis of pmTSP-II	426:457	A molecular analysis of pmTSP-II demonstrated anchorage sites for N-linked glycans in both the chitin-binding and TSP3 domains.
30740837	2	44	theme	Penaeus	409:415	arg1	monodon					417:423	the black tiger shrimp Penaeus monodon	386:423	the black tiger shrimp Penaeus monodon	386:423	Here, the presence of N-linked mannose (Man) glycoconjugates on shrimp thrombospondin ( pmTSP-II) and their physiological functions were investigated in the black tiger shrimp Penaeus monodon.
30740837	0	45	theme	shrimp	36:41	arg1	thrombospondin					43:56	shrimp thrombospondin	36:56	shrimp thrombospondin	36:56	N-linked mannose glycoconjugates on shrimp thrombospondin, pmTSP-II, and their involvement in the sperm acrosome reaction.
30740837	7	46	theme	reproductive	1332:1343	arg1	biology					1345:1351	shrimp reproductive biology	1325:1351	shrimp reproductive biology	1325:1351	Together, these results provide evidence of the pivotal role that Man-linked pmTSP-II plays in modulating the shrimp sperm AR, a novel role for a TSP family protein in shrimp reproductive biology.
30740837	4	47	dep	verified	587:594	arg1	250 kDa					674:680	250 kDa	674:680	250 kDa	674:680	The presence of Man residues was verified by concanavalin A lectin histochemistry on the purified fraction of pmTSP-II (250 kDa with protease inhibitor).
30740837	7	48	theme	shrimp	1325:1330	arg1	biology					1345:1351	shrimp reproductive biology	1325:1351	shrimp reproductive biology	1325:1351	Together, these results provide evidence of the pivotal role that Man-linked pmTSP-II plays in modulating the shrimp sperm AR, a novel role for a TSP family protein in shrimp reproductive biology.
30740837	2	49	theme	shrimp	402:407	arg1	monodon					417:423	the black tiger shrimp Penaeus monodon	386:423	the black tiger shrimp Penaeus monodon	386:423	Here, the presence of N-linked mannose (Man) glycoconjugates on shrimp thrombospondin ( pmTSP-II) and their physiological functions were investigated in the black tiger shrimp Penaeus monodon.
30740837	7	50	link	Man-linked	1223:1232	arg1	pmTSP-II					1234:1241	Man-linked pmTSP-II	1223:1241	Man-linked pmTSP-II	1223:1241	Together, these results provide evidence of the pivotal role that Man-linked pmTSP-II plays in modulating the shrimp sperm AR, a novel role for a TSP family protein in shrimp reproductive biology.
30740837	7	51	theme	family	1307:1312	arg1	protein					1314:1320	a TSP family protein	1301:1320	a TSP family protein	1301:1320	Together, these results provide evidence of the pivotal role that Man-linked pmTSP-II plays in modulating the shrimp sperm AR, a novel role for a TSP family protein in shrimp reproductive biology.
30740837	5	52	theme	pmTSP-II-induced	795:810	arg1	reaction					821:828	the pmTSP-II-induced acrosome reaction	791:828	the pmTSP-II-induced acrosome reaction (AR)	791:833	The function of the Man glycoconjugates was evident by the Con A interference with the pmTSP-II-induced acrosome reaction (AR) as well as by the ability to recover the induction of the AR by the inclusion of Mans in the treatment mixture.
30740837	5	52	theme	pmTSP-II-induced	795:810	arg1	AR					831:832	AR	831:832	AR	831:832	The function of the Man glycoconjugates was evident by the Con A interference with the pmTSP-II-induced acrosome reaction (AR) as well as by the ability to recover the induction of the AR by the inclusion of Mans in the treatment mixture.
30740837	3	53	link	N-linked	492:499	arg1	glycans					501:507	N-linked glycans	492:507	N-linked glycans	492:507	A molecular analysis of pmTSP-II demonstrated anchorage sites for N-linked glycans in both the chitin-binding and TSP3 domains.
30740837	6	54	theme	minimal	1112:1118	arg1	ability					1120:1126	a minimal ability	1110:1126	a minimal ability to initiate the AR response	1110:1154	In addition, the recombinant proteins of the three signature pmTSP-II domains expressed in E. coli (lacking glycosylation) and mannosidase-treated pmTSP-II showed a minimal ability to initiate the AR response.
30740837	1	55	from	functions	196:204	arg1	processes					222:230	reproductive processes	209:230	reproductive processes	209:230	Glycoconjugates in egg extracellular matrices are known to serve several functions in reproductive processes.
30740837	5	56	theme	AR	893:894	arg1	induction					876:884	the induction	872:884	the induction of the AR by the inclusion of Mans in the treatment mixture	872:944	The function of the Man glycoconjugates was evident by the Con A interference with the pmTSP-II-induced acrosome reaction (AR) as well as by the ability to recover the induction of the AR by the inclusion of Mans in the treatment mixture.
30740837	5	57	from	Mans	916:919	arg1	mixture					938:944	the treatment mixture	924:944	the treatment mixture	924:944	The function of the Man glycoconjugates was evident by the Con A interference with the pmTSP-II-induced acrosome reaction (AR) as well as by the ability to recover the induction of the AR by the inclusion of Mans in the treatment mixture.
30740837	2	58	theme	mannose	264:270	arg1	glycoconjugates					278:292	N-linked mannose (Man) glycoconjugates	255:292	N-linked mannose (Man) glycoconjugates	255:292	Here, the presence of N-linked mannose (Man) glycoconjugates on shrimp thrombospondin ( pmTSP-II) and their physiological functions were investigated in the black tiger shrimp Penaeus monodon.
30740837	3	59	from	sites	482:486	arg1	chitin-binding					521:534	chitin-binding	521:534	chitin-binding	521:534	A molecular analysis of pmTSP-II demonstrated anchorage sites for N-linked glycans in both the chitin-binding and TSP3 domains.
30740837	3	59	from	sites	482:486	arg1	domains					545:551	TSP3 domains	540:551	TSP3 domains	540:551	A molecular analysis of pmTSP-II demonstrated anchorage sites for N-linked glycans in both the chitin-binding and TSP3 domains.
30740837	6	60	theme	mannosidase-treated	1074:1092	arg1	pmTSP-II					1094:1101	mannosidase-treated pmTSP-II	1074:1101	mannosidase-treated pmTSP-II	1074:1101	In addition, the recombinant proteins of the three signature pmTSP-II domains expressed in E. coli (lacking glycosylation) and mannosidase-treated pmTSP-II showed a minimal ability to initiate the AR response.
30740837	5	61	theme	Mans	916:919	arg1	inclusion					903:911	the inclusion	899:911	the inclusion of Mans in the treatment mixture	899:944	The function of the Man glycoconjugates was evident by the Con A interference with the pmTSP-II-induced acrosome reaction (AR) as well as by the ability to recover the induction of the AR by the inclusion of Mans in the treatment mixture.
30740837	4	62	theme	lectin	614:619	arg1	histochemistry					621:634	concanavalin A lectin histochemistry	599:634	concanavalin A lectin histochemistry	599:634	The presence of Man residues was verified by concanavalin A lectin histochemistry on the purified fraction of pmTSP-II (250 kDa with protease inhibitor).
30740837	2	63	theme	N-linked	255:262	arg1	Man					273:275	Man	273:275	Man	273:275	Here, the presence of N-linked mannose (Man) glycoconjugates on shrimp thrombospondin ( pmTSP-II) and their physiological functions were investigated in the black tiger shrimp Penaeus monodon.
30740837	2	63	theme	N-linked	255:262	arg1	mannose					264:270	N-linked mannose	255:270	N-linked mannose (Man) glycoconjugates	255:292	Here, the presence of N-linked mannose (Man) glycoconjugates on shrimp thrombospondin ( pmTSP-II) and their physiological functions were investigated in the black tiger shrimp Penaeus monodon.
30740837	3	64	theme	N-linked	492:499	arg1	glycans					501:507	N-linked glycans	492:507	N-linked glycans	492:507	A molecular analysis of pmTSP-II demonstrated anchorage sites for N-linked glycans in both the chitin-binding and TSP3 domains.
30740837	5	65	from	inclusion	903:911	arg1	mixture					938:944	the treatment mixture	924:944	the treatment mixture	924:944	The function of the Man glycoconjugates was evident by the Con A interference with the pmTSP-II-induced acrosome reaction (AR) as well as by the ability to recover the induction of the AR by the inclusion of Mans in the treatment mixture.
30740837	4	66	theme	A	612:612	arg1	histochemistry					621:634	concanavalin A lectin histochemistry	599:634	concanavalin A lectin histochemistry	599:634	The presence of Man residues was verified by concanavalin A lectin histochemistry on the purified fraction of pmTSP-II (250 kDa with protease inhibitor).
30740837	7	67	theme	sperm	1274:1278	arg1	AR					1280:1281	the shrimp sperm AR	1263:1281	the shrimp sperm AR	1263:1281	Together, these results provide evidence of the pivotal role that Man-linked pmTSP-II plays in modulating the shrimp sperm AR, a novel role for a TSP family protein in shrimp reproductive biology.
30740837	3	68	theme	pmTSP-II	450:457	arg1	analysis					438:445	A molecular analysis	426:445	A molecular analysis of pmTSP-II	426:457	A molecular analysis of pmTSP-II demonstrated anchorage sites for N-linked glycans in both the chitin-binding and TSP3 domains.
30740837	4	69	theme	purified	643:650	arg1	fraction					652:659	the purified fraction	639:659	the purified fraction of pmTSP-II	639:671	The presence of Man residues was verified by concanavalin A lectin histochemistry on the purified fraction of pmTSP-II (250 kDa with protease inhibitor).
30740837	1	70	theme	egg	142:144	arg1	matrices					160:167	egg extracellular matrices	142:167	egg extracellular matrices	142:167	Glycoconjugates in egg extracellular matrices are known to serve several functions in reproductive processes.
30740837	4	71	theme	pmTSP-II	664:671	arg1	fraction					652:659	the purified fraction	639:659	the purified fraction of pmTSP-II	639:671	The presence of Man residues was verified by concanavalin A lectin histochemistry on the purified fraction of pmTSP-II (250 kDa with protease inhibitor).
30740837	2	72	attach	presence	243:250	arg1	functions					355:363	their physiological functions	335:363	their physiological functions	335:363	Here, the presence of N-linked mannose (Man) glycoconjugates on shrimp thrombospondin ( pmTSP-II) and their physiological functions were investigated in the black tiger shrimp Penaeus monodon.
30740837	2	72	attach	presence	243:250	arg1	pmTSP-II					321:328	pmTSP-II	321:328	pmTSP-II	321:328	Here, the presence of N-linked mannose (Man) glycoconjugates on shrimp thrombospondin ( pmTSP-II) and their physiological functions were investigated in the black tiger shrimp Penaeus monodon.
30740837	2	72	attach	presence	243:250	arg1	thrombospondin					304:317	shrimp thrombospondin	297:317	shrimp thrombospondin ( pmTSP-II)	297:329	Here, the presence of N-linked mannose (Man) glycoconjugates on shrimp thrombospondin ( pmTSP-II) and their physiological functions were investigated in the black tiger shrimp Penaeus monodon.
30740837	2	72	attach	presence	243:250	arg2	glycoconjugates					278:292	N-linked mannose (Man) glycoconjugates	255:292	N-linked mannose (Man) glycoconjugates	255:292	Here, the presence of N-linked mannose (Man) glycoconjugates on shrimp thrombospondin ( pmTSP-II) and their physiological functions were investigated in the black tiger shrimp Penaeus monodon.
30740837	7	73	theme	Man-linked	1223:1232	arg1	pmTSP-II					1234:1241	Man-linked pmTSP-II	1223:1241	Man-linked pmTSP-II	1223:1241	Together, these results provide evidence of the pivotal role that Man-linked pmTSP-II plays in modulating the shrimp sperm AR, a novel role for a TSP family protein in shrimp reproductive biology.
30740837	1	74	theme	extracellular	146:158	arg1	matrices					160:167	egg extracellular matrices	142:167	egg extracellular matrices	142:167	Glycoconjugates in egg extracellular matrices are known to serve several functions in reproductive processes.
30740837	5	75	from	mixture	938:944	arg1	inclusion					903:911	the inclusion	899:911	the inclusion of Mans in the treatment mixture	899:944	The function of the Man glycoconjugates was evident by the Con A interference with the pmTSP-II-induced acrosome reaction (AR) as well as by the ability to recover the induction of the AR by the inclusion of Mans in the treatment mixture.
30740837	6	76	theme	recombinant	964:974	arg1	proteins					976:983	the recombinant proteins	960:983	the recombinant proteins of the three signature pmTSP-II domains expressed in E. coli (lacking glycosylation) and mannosidase-treated pmTSP-II	960:1101	In addition, the recombinant proteins of the three signature pmTSP-II domains expressed in E. coli (lacking glycosylation) and mannosidase-treated pmTSP-II showed a minimal ability to initiate the AR response.
31316996	10	0	theme	worm	1396:1399	arg1	sizes					1401:1405	worm sizes	1396:1405	worm sizes	1396:1405	Furthermore, some metabolites that were correlated to these glycans and worm sizes showed interesting interactions.
31316996	7	1	theme	adult	1002:1006	arg1	animals					1008:1014	adult animals	1002:1014	adult animals	1002:1014	From the five time points, four distinct sizes of worms were observed from the Biosorter distributions, ranging from the smallest corresponding to L1 to adult animals.
31316996	9	2	with	consistent	1290:1299	arg1	studies					1315:1321	previous studies	1306:1321	previous studies	1306:1321	The emerging structure of the network showed distinct patterns of N- and O-linked glycans that were consistent with previous studies.
31316996	4	3	theme	O-linked	553:560	arg1	glycans					562:568	O-linked glycans	553:568	O-linked glycans	553:568	The samples were each assayed by a large particle flow cytometer (Biosorter) for size distribution data, LC-MS/MS for targeted N- and O-linked glycans, and NMR for metabolites.
31316996	9	4	theme	distinct	1235:1242	arg1	patterns					1244:1251	distinct patterns	1235:1251	distinct patterns of N- and O-linked glycans that were consistent with previous studies	1235:1321	The emerging structure of the network showed distinct patterns of N- and O-linked glycans that were consistent with previous studies.
31316996	7	5	theme	Biosorter	928:936	arg1	distributions					938:950	the Biosorter distributions	924:950	the Biosorter distributions	924:950	From the five time points, four distinct sizes of worms were observed from the Biosorter distributions, ranging from the smallest corresponding to L1 to adult animals.
31316996	2	6	theme	adults	352:357	arg1	population					323:332	a mixed population	315:332	a mixed population of adults, gravid adults, and offspring	315:372	Samples of N2 animals were synchronized and grown to five different time points ranging from L1 to a mixed population of adults, gravid adults, and offspring.
31316996	0	7	from	Correlations	0:11	arg1	elegans					97:103	Caenorhabditis elegans	82:103	Caenorhabditis elegans	82:103	Correlations Between LC-MS/MS-Detected Glycomics and NMR-Detected Metabolomics in Caenorhabditis elegans Development.
31316996	1	8	from	development	189:199	arg1	elegans					207:213	C. elegans	204:213	C. elegans	204:213	This study examined the relationship between glycans, metabolites, and development in C. elegans.
31316996	4	9	dep	assayed	441:447	arg1	Biosorter					485:493	Biosorter	485:493	Biosorter	485:493	The samples were each assayed by a large particle flow cytometer (Biosorter) for size distribution data, LC-MS/MS for targeted N- and O-linked glycans, and NMR for metabolites.
31316996	4	9	dep	assayed	441:447	arg1	each					436:439	each	436:439	each	436:439	The samples were each assayed by a large particle flow cytometer (Biosorter) for size distribution data, LC-MS/MS for targeted N- and O-linked glycans, and NMR for metabolites.
31316996	4	10	theme	particle	460:467	arg1	cytometer					474:482	a large particle flow cytometer	452:482	a large particle flow cytometer	452:482	The samples were each assayed by a large particle flow cytometer (Biosorter) for size distribution data, LC-MS/MS for targeted N- and O-linked glycans, and NMR for metabolites.
31316996	1	11	from	metabolites	172:182	arg1	elegans					207:213	C. elegans	204:213	C. elegans	204:213	This study examined the relationship between glycans, metabolites, and development in C. elegans.
31316996	5	12	used	utilized	618:625	arg2	samples					605:611	The same samples	596:611	The same samples	596:611	The same samples were utilized for all measurements, which allowed for statistical correlations between the data.
31316996	4	13	theme	large	454:458	arg1	cytometer					474:482	a large particle flow cytometer	452:482	a large particle flow cytometer	452:482	The samples were each assayed by a large particle flow cytometer (Biosorter) for size distribution data, LC-MS/MS for targeted N- and O-linked glycans, and NMR for metabolites.
31316996	8	14	theme	network	1019:1025	arg1	model					1027:1031	A network model	1017:1031	A network model	1017:1031	A network model was constructed using the four binned sizes of worms as starting nodes and adding glycans and metabolites that had correlations with r ≥ 0.5 to those nodes.
31316996	11	15	theme	positive	1471:1478	arg1	correlations					1480:1491	strong positive correlations	1464:1491	strong positive correlations	1464:1491	Of note, UDP-GlcNAc had strong positive correlations with many O-glycans that were expressed in the largest animals.
31316996	9	16	link	O-linked	1263:1270	arg1	glycans					1272:1278	O-linked glycans	1263:1278	O-linked glycans	1263:1278	The emerging structure of the network showed distinct patterns of N- and O-linked glycans that were consistent with previous studies.
31316996	10	17	theme	interesting	1414:1424	arg1	interactions					1426:1437	interesting interactions	1414:1437	interesting interactions	1414:1437	Furthermore, some metabolites that were correlated to these glycans and worm sizes showed interesting interactions.
31316996	1	18	from	glycans	163:169	arg1	elegans					207:213	C. elegans	204:213	C. elegans	204:213	This study examined the relationship between glycans, metabolites, and development in C. elegans.
31316996	12	19	theme	L1	1640:1641	arg1	animals					1643:1649	L1 animals	1640:1649	L1 animals	1640:1649	Similarly, phosphorylcholine correlated with many N-glycans that were expressed in L1 animals.
31316996	1	20	theme	C.	204:205	arg1	elegans					207:213	C. elegans	204:213	C. elegans	204:213	This study examined the relationship between glycans, metabolites, and development in C. elegans.
31316996	3	21	theme	time	380:383	arg1	point					385:389	Each time point	375:389	Each time point	375:389	Each time point was replicated seven times.
31316996	4	22	theme	flow	469:472	arg1	cytometer					474:482	a large particle flow cytometer	452:482	a large particle flow cytometer	452:482	The samples were each assayed by a large particle flow cytometer (Biosorter) for size distribution data, LC-MS/MS for targeted N- and O-linked glycans, and NMR for metabolites.
31316996	5	23	theme	same	600:603	arg1	samples					605:611	The same samples	596:611	The same samples	596:611	The same samples were utilized for all measurements, which allowed for statistical correlations between the data.
31316996	6	24	theme	Biosorter	752:760	arg1	data					776:779	Biosorter developmental data	752:779	Biosorter developmental data	752:779	A new protocol was developed to correlate Biosorter developmental data with LC-MS/MS data to obtain stage-specific information of glycans.
31316996	9	25	theme	N-	1256:1257	arg1	patterns					1244:1251	distinct patterns	1235:1251	distinct patterns of N- and O-linked glycans that were consistent with previous studies	1235:1321	The emerging structure of the network showed distinct patterns of N- and O-linked glycans that were consistent with previous studies.
31316996	9	26	theme	glycans	1272:1278	arg1	patterns					1244:1251	distinct patterns	1235:1251	distinct patterns of N- and O-linked glycans that were consistent with previous studies	1235:1321	The emerging structure of the network showed distinct patterns of N- and O-linked glycans that were consistent with previous studies.
31316996	0	27	dep	Development	105:115	arg1	Correlations					0:11	Correlations	0:11	Correlations Between LC-MS/MS-Detected Glycomics and NMR-Detected Metabolomics in Caenorhabditis elegans	0:103	Correlations Between LC-MS/MS-Detected Glycomics and NMR-Detected Metabolomics in Caenorhabditis elegans Development.
31316996	6	28	theme	developmental	762:774	arg1	data					776:779	Biosorter developmental data	752:779	Biosorter developmental data	752:779	A new protocol was developed to correlate Biosorter developmental data with LC-MS/MS data to obtain stage-specific information of glycans.
31316996	7	29	theme	worms	899:903	arg1	sizes					890:894	four distinct sizes	876:894	four distinct sizes of worms	876:903	From the five time points, four distinct sizes of worms were observed from the Biosorter distributions, ranging from the smallest corresponding to L1 to adult animals.
31316996	11	30	theme	many	1498:1501	arg1	O-glycans					1503:1511	many O-glycans	1498:1511	many O-glycans that were expressed in the largest animals	1498:1554	Of note, UDP-GlcNAc had strong positive correlations with many O-glycans that were expressed in the largest animals.
31316996	2	31	theme	mixed	317:321	arg1	population					323:332	a mixed population	315:332	a mixed population of adults, gravid adults, and offspring	315:372	Samples of N2 animals were synchronized and grown to five different time points ranging from L1 to a mixed population of adults, gravid adults, and offspring.
31316996	5	32	theme	statistical	667:677	arg1	correlations					679:690	statistical correlations	667:690	statistical correlations between the data	667:707	The same samples were utilized for all measurements, which allowed for statistical correlations between the data.
31316996	11	33	contain	had	1460:1462	arg2	correlations					1480:1491	strong positive correlations	1464:1491	strong positive correlations	1464:1491	Of note, UDP-GlcNAc had strong positive correlations with many O-glycans that were expressed in the largest animals.
31316996	11	33	contain	had	1460:1462	arg1	UDP-GlcNAc					1449:1458	UDP-GlcNAc	1449:1458	UDP-GlcNAc	1449:1458	Of note, UDP-GlcNAc had strong positive correlations with many O-glycans that were expressed in the largest animals.
31316996	12	34	theme	many	1602:1605	arg1	N-glycans					1607:1615	many N-glycans	1602:1615	many N-glycans that were expressed in L1 animals	1602:1649	Similarly, phosphorylcholine correlated with many N-glycans that were expressed in L1 animals.
31316996	0	35	theme	NMR-Detected	53:64	arg1	Metabolomics					66:77	NMR-Detected Metabolomics	53:77	NMR-Detected Metabolomics	53:77	Correlations Between LC-MS/MS-Detected Glycomics and NMR-Detected Metabolomics in Caenorhabditis elegans Development.
31316996	4	36	theme	targeted	537:544	arg1	N-					546:547	targeted N-	537:547	targeted N-	537:547	The samples were each assayed by a large particle flow cytometer (Biosorter) for size distribution data, LC-MS/MS for targeted N- and O-linked glycans, and NMR for metabolites.
31316996	8	37	theme	r	1166:1166	arg1	≥					1168:1168	r ≥ 0.5	1166:1172	r ≥ 0.5 to those nodes	1166:1187	A network model was constructed using the four binned sizes of worms as starting nodes and adding glycans and metabolites that had correlations with r ≥ 0.5 to those nodes.
31316996	4	38	theme	distribution	505:516	arg1	data					518:521	size distribution data	500:521	size distribution data	500:521	The samples were each assayed by a large particle flow cytometer (Biosorter) for size distribution data, LC-MS/MS for targeted N- and O-linked glycans, and NMR for metabolites.
31316996	6	39	theme	glycans	840:846	arg1	information					825:835	stage-specific information	810:835	stage-specific information of glycans	810:846	A new protocol was developed to correlate Biosorter developmental data with LC-MS/MS data to obtain stage-specific information of glycans.
31316996	4	40	theme	size	500:503	arg1	data					518:521	size distribution data	500:521	size distribution data	500:521	The samples were each assayed by a large particle flow cytometer (Biosorter) for size distribution data, LC-MS/MS for targeted N- and O-linked glycans, and NMR for metabolites.
31316996	8	41	with	correlations	1148:1159	arg1	≥					1168:1168	r ≥ 0.5	1166:1172	r ≥ 0.5 to those nodes	1166:1187	A network model was constructed using the four binned sizes of worms as starting nodes and adding glycans and metabolites that had correlations with r ≥ 0.5 to those nodes.
31316996	2	42	theme	gravid	345:350	arg1	adults					352:357	gravid adults	345:357	gravid adults	345:357	Samples of N2 animals were synchronized and grown to five different time points ranging from L1 to a mixed population of adults, gravid adults, and offspring.
31316996	9	43	theme	emerging	1194:1201	arg1	structure					1203:1211	The emerging structure	1190:1211	The emerging structure of the network	1190:1226	The emerging structure of the network showed distinct patterns of N- and O-linked glycans that were consistent with previous studies.
31316996	8	44	theme	binned	1064:1069	arg1	sizes					1071:1075	the four binned sizes	1055:1075	the four binned sizes of worms	1055:1084	A network model was constructed using the four binned sizes of worms as starting nodes and adding glycans and metabolites that had correlations with r ≥ 0.5 to those nodes.
31316996	2	45	theme	animals	230:236	arg1	Samples					216:222	Samples	216:222	Samples of N2 animals	216:236	Samples of N2 animals were synchronized and grown to five different time points ranging from L1 to a mixed population of adults, gravid adults, and offspring.
31316996	6	46	theme	stage-specific	810:823	arg1	information					825:835	stage-specific information	810:835	stage-specific information of glycans	810:846	A new protocol was developed to correlate Biosorter developmental data with LC-MS/MS data to obtain stage-specific information of glycans.
31316996	7	47	theme	distinct	881:888	arg1	sizes					890:894	four distinct sizes	876:894	four distinct sizes of worms	876:903	From the five time points, four distinct sizes of worms were observed from the Biosorter distributions, ranging from the smallest corresponding to L1 to adult animals.
31316996	8	48	contain	had	1144:1146	arg1	glycans					1115:1121	glycans	1115:1121	glycans	1115:1121	A network model was constructed using the four binned sizes of worms as starting nodes and adding glycans and metabolites that had correlations with r ≥ 0.5 to those nodes.
31316996	8	48	contain	had	1144:1146	arg1	metabolites					1127:1137	metabolites	1127:1137	metabolites	1127:1137	A network model was constructed using the four binned sizes of worms as starting nodes and adding glycans and metabolites that had correlations with r ≥ 0.5 to those nodes.
31316996	8	48	contain	had	1144:1146	arg2	correlations					1148:1159	correlations	1148:1159	correlations with r ≥ 0.5 to those nodes	1148:1187	A network model was constructed using the four binned sizes of worms as starting nodes and adding glycans and metabolites that had correlations with r ≥ 0.5 to those nodes.
31316996	8	49	theme	worms	1080:1084	arg1	sizes					1071:1075	the four binned sizes	1055:1075	the four binned sizes of worms	1055:1084	A network model was constructed using the four binned sizes of worms as starting nodes and adding glycans and metabolites that had correlations with r ≥ 0.5 to those nodes.
31316996	2	50	theme	N2	227:228	arg1	animals					230:236	N2 animals	227:236	N2 animals	227:236	Samples of N2 animals were synchronized and grown to five different time points ranging from L1 to a mixed population of adults, gravid adults, and offspring.
31316996	2	51	theme	time	284:287	arg1	points					289:294	five different time points	269:294	five different time points ranging from L1 to a mixed population of adults, gravid adults, and offspring	269:372	Samples of N2 animals were synchronized and grown to five different time points ranging from L1 to a mixed population of adults, gravid adults, and offspring.
31316996	6	52	theme	LC-MS/MS	786:793	arg1	data					795:798	LC-MS/MS data	786:798	LC-MS/MS data	786:798	A new protocol was developed to correlate Biosorter developmental data with LC-MS/MS data to obtain stage-specific information of glycans.
31316996	2	53	theme	adults	337:342	arg1	population					323:332	a mixed population	315:332	a mixed population of adults, gravid adults, and offspring	315:372	Samples of N2 animals were synchronized and grown to five different time points ranging from L1 to a mixed population of adults, gravid adults, and offspring.
31316996	6	54	theme	new	712:714	arg1	protocol					716:723	A new protocol	710:723	A new protocol	710:723	A new protocol was developed to correlate Biosorter developmental data with LC-MS/MS data to obtain stage-specific information of glycans.
31316996	4	55	link	O-linked	553:560	arg1	glycans					562:568	O-linked glycans	553:568	O-linked glycans	553:568	The samples were each assayed by a large particle flow cytometer (Biosorter) for size distribution data, LC-MS/MS for targeted N- and O-linked glycans, and NMR for metabolites.
31316996	2	56	theme	different	274:282	arg1	points					289:294	five different time points	269:294	five different time points ranging from L1 to a mixed population of adults, gravid adults, and offspring	269:372	Samples of N2 animals were synchronized and grown to five different time points ranging from L1 to a mixed population of adults, gravid adults, and offspring.
31316996	11	57	theme	strong	1464:1469	arg1	correlations					1480:1491	strong positive correlations	1464:1491	strong positive correlations	1464:1491	Of note, UDP-GlcNAc had strong positive correlations with many O-glycans that were expressed in the largest animals.
31316996	2	58	theme	offspring	364:372	arg1	population					323:332	a mixed population	315:332	a mixed population of adults, gravid adults, and offspring	315:372	Samples of N2 animals were synchronized and grown to five different time points ranging from L1 to a mixed population of adults, gravid adults, and offspring.
31316996	11	59	theme	largest	1540:1546	arg1	animals					1548:1554	the largest animals	1536:1554	the largest animals	1536:1554	Of note, UDP-GlcNAc had strong positive correlations with many O-glycans that were expressed in the largest animals.
31316996	0	60	theme	Caenorhabditis	82:95	arg1	elegans					97:103	Caenorhabditis elegans	82:103	Caenorhabditis elegans	82:103	Correlations Between LC-MS/MS-Detected Glycomics and NMR-Detected Metabolomics in Caenorhabditis elegans Development.
31316996	9	61	theme	network	1220:1226	arg1	structure					1203:1211	The emerging structure	1190:1211	The emerging structure of the network	1190:1226	The emerging structure of the network showed distinct patterns of N- and O-linked glycans that were consistent with previous studies.
31316996	0	62	theme	LC-MS/MS-Detected	21:37	arg1	Glycomics					39:47	LC-MS/MS-Detected Glycomics	21:47	LC-MS/MS-Detected Glycomics	21:47	Correlations Between LC-MS/MS-Detected Glycomics and NMR-Detected Metabolomics in Caenorhabditis elegans Development.
31316996	9	63	theme	O-linked	1263:1270	arg1	glycans					1272:1278	O-linked glycans	1263:1278	O-linked glycans	1263:1278	The emerging structure of the network showed distinct patterns of N- and O-linked glycans that were consistent with previous studies.
31316996	7	64	theme	time	863:866	arg1	points					868:873	the five time points	854:873	the five time points	854:873	From the five time points, four distinct sizes of worms were observed from the Biosorter distributions, ranging from the smallest corresponding to L1 to adult animals.
31316996	9	65	theme	previous	1306:1313	arg1	studies					1315:1321	previous studies	1306:1321	previous studies	1306:1321	The emerging structure of the network showed distinct patterns of N- and O-linked glycans that were consistent with previous studies.
30835280	2	0	theme	eukaryotes-are	508:521	arg1	present					523:529	eukaryotes-are present	508:529	eukaryotes-are present in Plasmodium species	508:551	Nevertheless, the currently established notion is that only four out of eight subunits of the OST complex-which is considered conserved across eukaryotes-are present in Plasmodium species.
30835280	0	1	from	view	11:14	arg1	Plasmodium					60:69	Plasmodium	60:69	Plasmodium	60:69	An updated view of the oligosaccharyltransferase complex in Plasmodium.
30835280	4	2	theme	protein	904:910	arg1	sequences					912:920	uncharacterized protein sequences	888:920	uncharacterized protein sequences already existing in sequence databases	888:959	In fact, most of the subunits currently considered as absent from Plasmodium refer to uncharacterized protein sequences already existing in sequence databases.
30835280	5	3	theme	coding	1198:1203	arg1	sequence					1205:1212	such a short coding sequence	1185:1212	such a short coding sequence	1185:1212	Interestingly, the main reason why the unusually short Ost4 subunit (36 residues long in yeast) has not been identified so far in plasmodia (and possibly other species) is the failure of gene-prediction pipelines to detect such a short coding sequence.
30835280	1	4	theme	parasites	354:362	arg1	genomes					335:341	completely sequenced genomes	314:341	completely sequenced genomes of malaria parasites	314:362	Despite the controversy regarding the importance of protein N-linked glycosylation in species of the genus Plasmodium, genes potentially encoding core subunits of the oligosaccharyltransferase (OST) complex have already been characterized in completely sequenced genomes of malaria parasites.
30835280	3	5	theme	Swp1/Ribophorin	707:721	arg1	exception					694:702	the exception	690:702	the exception of Swp1/Ribophorin II	690:724	In this study, we carefully conduct computational analysis to provide unequivocal evidence that all components of the OST complex, with the exception of Swp1/Ribophorin II, can be reliably identified within completely sequenced plasmodial genomes.
30835280	4	6	theme	uncharacterized	888:902	arg1	sequences					912:920	uncharacterized protein sequences	888:920	uncharacterized protein sequences already existing in sequence databases	888:959	In fact, most of the subunits currently considered as absent from Plasmodium refer to uncharacterized protein sequences already existing in sequence databases.
30835280	3	7	theme	OST	672:674	arg1	complex					676:682	the OST complex	668:682	the OST complex	668:682	In this study, we carefully conduct computational analysis to provide unequivocal evidence that all components of the OST complex, with the exception of Swp1/Ribophorin II, can be reliably identified within completely sequenced plasmodial genomes.
30835280	6	8	theme	sequenced	1298:1306	arg1	genomes					1308:1314	completely sequenced genomes	1287:1314	completely sequenced genomes	1287:1314	We further identify elusive OST subunits in select protist species with completely sequenced genomes.
30835280	7	9	theme	systematic	1362:1371	arg1	approach					1373:1380	a systematic approach	1360:1380	a systematic approach	1360:1380	Thus, our work highlights the necessity of a systematic approach towards the characterization of OST subunits across eukaryotes.
30835280	5	10	dep	species	1122:1128	arg1	possibly					1107:1114	possibly	1107:1114	possibly	1107:1114	Interestingly, the main reason why the unusually short Ost4 subunit (36 residues long in yeast) has not been identified so far in plasmodia (and possibly other species) is the failure of gene-prediction pipelines to detect such a short coding sequence.
30835280	8	11	theme	evolution	1509:1517	arg1	picture					1494:1500	a concrete picture	1483:1500	a concrete picture of the evolution of the OST complex	1483:1536	This is necessary both for obtaining a concrete picture of the evolution of the OST complex but also for elucidating its possible role in eukaryotic pathogens.
30835280	1	12	theme	complex	271:277	arg1	subunits					223:230	core subunits	218:230	core subunits of the oligosaccharyltransferase (OST) complex	218:277	Despite the controversy regarding the importance of protein N-linked glycosylation in species of the genus Plasmodium, genes potentially encoding core subunits of the oligosaccharyltransferase (OST) complex have already been characterized in completely sequenced genomes of malaria parasites.
30835280	5	13	theme	main	981:984	arg1	reason					986:991	the main reason	977:991	the main reason why the unusually short Ost4 subunit (36 residues long in yeast) has not been identified so far in plasmodia (and possibly other species)	977:1129	Interestingly, the main reason why the unusually short Ost4 subunit (36 residues long in yeast) has not been identified so far in plasmodia (and possibly other species) is the failure of gene-prediction pipelines to detect such a short coding sequence.
30835280	5	13	theme	main	981:984	arg1	failure					1138:1144	the failure	1134:1144	the failure of gene-prediction pipelines to detect such a short coding sequence	1134:1212	Interestingly, the main reason why the unusually short Ost4 subunit (36 residues long in yeast) has not been identified so far in plasmodia (and possibly other species) is the failure of gene-prediction pipelines to detect such a short coding sequence.
30835280	8	14	theme	complex	1530:1536	arg1	evolution					1509:1517	the evolution	1505:1517	the evolution of the OST complex	1505:1536	This is necessary both for obtaining a concrete picture of the evolution of the OST complex but also for elucidating its possible role in eukaryotic pathogens.
30835280	2	15	dep	considered	480:489	arg1	conserved					491:499	conserved	491:499	is considered conserved across eukaryotes-are present in Plasmodium species	477:551	Nevertheless, the currently established notion is that only four out of eight subunits of the OST complex-which is considered conserved across eukaryotes-are present in Plasmodium species.
30835280	5	16	theme	short	1011:1015	arg1	subunit					1022:1028	the unusually short Ost4 subunit	997:1028	the unusually short Ost4 subunit (36 residues long in yeast)	997:1056	Interestingly, the main reason why the unusually short Ost4 subunit (36 residues long in yeast) has not been identified so far in plasmodia (and possibly other species) is the failure of gene-prediction pipelines to detect such a short coding sequence.
30835280	7	17	theme	subunits	1418:1425	arg1	characterization					1394:1409	the characterization	1390:1409	the characterization of OST subunits across eukaryotes	1390:1443	Thus, our work highlights the necessity of a systematic approach towards the characterization of OST subunits across eukaryotes.
30835280	8	18	theme	OST	1526:1528	arg1	complex					1530:1536	the OST complex	1522:1536	the OST complex	1522:1536	This is necessary both for obtaining a concrete picture of the evolution of the OST complex but also for elucidating its possible role in eukaryotic pathogens.
30835280	6	19	theme	protist	1266:1272	arg1	species					1274:1280	select protist species	1259:1280	select protist species with completely sequenced genomes	1259:1314	We further identify elusive OST subunits in select protist species with completely sequenced genomes.
30835280	5	20	dep	subunit	1022:1028	arg1	residues					1034:1041	36 residues	1031:1041	36 residues long in yeast	1031:1055	Interestingly, the main reason why the unusually short Ost4 subunit (36 residues long in yeast) has not been identified so far in plasmodia (and possibly other species) is the failure of gene-prediction pipelines to detect such a short coding sequence.
30835280	5	21	theme	Ost4	1017:1020	arg1	subunit					1022:1028	the unusually short Ost4 subunit	997:1028	the unusually short Ost4 subunit (36 residues long in yeast)	997:1056	Interestingly, the main reason why the unusually short Ost4 subunit (36 residues long in yeast) has not been identified so far in plasmodia (and possibly other species) is the failure of gene-prediction pipelines to detect such a short coding sequence.
30835280	5	22	from	long	1043:1046	arg1	yeast					1051:1055	yeast	1051:1055	yeast	1051:1055	Interestingly, the main reason why the unusually short Ost4 subunit (36 residues long in yeast) has not been identified so far in plasmodia (and possibly other species) is the failure of gene-prediction pipelines to detect such a short coding sequence.
30835280	1	23	theme	genus	173:177	arg1	Plasmodium					179:188	the genus Plasmodium	169:188	the genus Plasmodium	169:188	Despite the controversy regarding the importance of protein N-linked glycosylation in species of the genus Plasmodium, genes potentially encoding core subunits of the oligosaccharyltransferase (OST) complex have already been characterized in completely sequenced genomes of malaria parasites.
30835280	2	24	theme	Plasmodium	534:543	arg1	species					545:551	Plasmodium species	534:551	Plasmodium species	534:551	Nevertheless, the currently established notion is that only four out of eight subunits of the OST complex-which is considered conserved across eukaryotes-are present in Plasmodium species.
30835280	4	25	from	Plasmodium	868:877	arg1	absent					856:861	absent	856:861	absent	856:861	In fact, most of the subunits currently considered as absent from Plasmodium refer to uncharacterized protein sequences already existing in sequence databases.
30835280	6	26	theme	select	1259:1264	arg1	species					1274:1280	select protist species	1259:1280	select protist species with completely sequenced genomes	1259:1314	We further identify elusive OST subunits in select protist species with completely sequenced genomes.
30835280	1	27	theme	Plasmodium	179:188	arg1	species					158:164	species	158:164	species of the genus Plasmodium	158:188	Despite the controversy regarding the importance of protein N-linked glycosylation in species of the genus Plasmodium, genes potentially encoding core subunits of the oligosaccharyltransferase (OST) complex have already been characterized in completely sequenced genomes of malaria parasites.
30835280	0	28	theme	updated	3:9	arg1	view					11:14	An updated view	0:14	An updated view of the oligosaccharyltransferase complex in Plasmodium	0:69	An updated view of the oligosaccharyltransferase complex in Plasmodium.
30835280	5	29	theme	short	1192:1196	arg1	sequence					1205:1212	such a short coding sequence	1185:1212	such a short coding sequence	1185:1212	Interestingly, the main reason why the unusually short Ost4 subunit (36 residues long in yeast) has not been identified so far in plasmodia (and possibly other species) is the failure of gene-prediction pipelines to detect such a short coding sequence.
30835280	3	30	theme	computational	590:602	arg1	analysis					604:611	computational analysis	590:611	computational analysis to provide unequivocal evidence that all components of the OST complex, with the exception of Swp1/Ribophorin II, can be reliably identified within completely sequenced plasmodial genomes	590:799	In this study, we carefully conduct computational analysis to provide unequivocal evidence that all components of the OST complex, with the exception of Swp1/Ribophorin II, can be reliably identified within completely sequenced plasmodial genomes.
30835280	3	31	theme	plasmodial	782:791	arg1	genomes					793:799	completely sequenced plasmodial genomes	761:799	completely sequenced plasmodial genomes	761:799	In this study, we carefully conduct computational analysis to provide unequivocal evidence that all components of the OST complex, with the exception of Swp1/Ribophorin II, can be reliably identified within completely sequenced plasmodial genomes.
30835280	8	32	from	role	1576:1579	arg1	pathogens					1595:1603	eukaryotic pathogens	1584:1603	eukaryotic pathogens	1584:1603	This is necessary both for obtaining a concrete picture of the evolution of the OST complex but also for elucidating its possible role in eukaryotic pathogens.
30835280	4	33	from	databases	951:959	arg1	existing					930:937	existing	930:937	existing	930:937	In fact, most of the subunits currently considered as absent from Plasmodium refer to uncharacterized protein sequences already existing in sequence databases.
30835280	6	34	theme	OST	1243:1245	arg1	subunits					1247:1254	elusive OST subunits	1235:1254	elusive OST subunits	1235:1254	We further identify elusive OST subunits in select protist species with completely sequenced genomes.
30835280	8	35	theme	possible	1567:1574	arg1	role					1576:1579	its possible role	1563:1579	its possible role in eukaryotic pathogens	1563:1603	This is necessary both for obtaining a concrete picture of the evolution of the OST complex but also for elucidating its possible role in eukaryotic pathogens.
30835280	3	36	theme	complex	676:682	arg1	components					654:663	all components	650:663	all components of the OST complex	650:682	In this study, we carefully conduct computational analysis to provide unequivocal evidence that all components of the OST complex, with the exception of Swp1/Ribophorin II, can be reliably identified within completely sequenced plasmodial genomes.
30835280	7	37	theme	OST	1414:1416	arg1	subunits					1418:1425	OST subunits	1414:1425	OST subunits across eukaryotes	1414:1443	Thus, our work highlights the necessity of a systematic approach towards the characterization of OST subunits across eukaryotes.
30835280	0	38	theme	oligosaccharyltransferase	23:47	arg1	complex					49:55	the oligosaccharyltransferase complex	19:55	the oligosaccharyltransferase complex	19:55	An updated view of the oligosaccharyltransferase complex in Plasmodium.
30835280	3	39	theme	sequenced	772:780	arg1	genomes					793:799	completely sequenced plasmodial genomes	761:799	completely sequenced plasmodial genomes	761:799	In this study, we carefully conduct computational analysis to provide unequivocal evidence that all components of the OST complex, with the exception of Swp1/Ribophorin II, can be reliably identified within completely sequenced plasmodial genomes.
30835280	3	40	theme	unequivocal	624:634	arg1	evidence					636:643	unequivocal evidence that all components of the OST complex, with the exception of Swp1/Ribophorin II, can be reliably identified within completely sequenced plasmodial genomes	624:799	unequivocal evidence that all components of the OST complex, with the exception of Swp1/Ribophorin II, can be reliably identified within completely sequenced plasmodial genomes	624:799	In this study, we carefully conduct computational analysis to provide unequivocal evidence that all components of the OST complex, with the exception of Swp1/Ribophorin II, can be reliably identified within completely sequenced plasmodial genomes.
30835280	5	41	from	yeast	1051:1055	arg1	long					1043:1046	long	1043:1046	long	1043:1046	Interestingly, the main reason why the unusually short Ost4 subunit (36 residues long in yeast) has not been identified so far in plasmodia (and possibly other species) is the failure of gene-prediction pipelines to detect such a short coding sequence.
30835280	1	42	theme	core	218:221	arg1	subunits					223:230	core subunits	218:230	core subunits of the oligosaccharyltransferase (OST) complex	218:277	Despite the controversy regarding the importance of protein N-linked glycosylation in species of the genus Plasmodium, genes potentially encoding core subunits of the oligosaccharyltransferase (OST) complex have already been characterized in completely sequenced genomes of malaria parasites.
30835280	6	43	theme	elusive	1235:1241	arg1	subunits					1247:1254	elusive OST subunits	1235:1254	elusive OST subunits	1235:1254	We further identify elusive OST subunits in select protist species with completely sequenced genomes.
30835280	8	44	dep	necessary	1454:1462	arg1	both					1464:1467	both	1464:1467	both	1464:1467	This is necessary both for obtaining a concrete picture of the evolution of the OST complex but also for elucidating its possible role in eukaryotic pathogens.
30835280	8	45	theme	concrete	1485:1492	arg1	picture					1494:1500	a concrete picture	1483:1500	a concrete picture of the evolution of the OST complex	1483:1536	This is necessary both for obtaining a concrete picture of the evolution of the OST complex but also for elucidating its possible role in eukaryotic pathogens.
30835280	1	46	theme	sequenced	325:333	arg1	genomes					335:341	completely sequenced genomes	314:341	completely sequenced genomes of malaria parasites	314:362	Despite the controversy regarding the importance of protein N-linked glycosylation in species of the genus Plasmodium, genes potentially encoding core subunits of the oligosaccharyltransferase (OST) complex have already been characterized in completely sequenced genomes of malaria parasites.
30835280	1	47	link	N-linked	132:139	arg1	glycosylation					141:153	protein N-linked glycosylation	124:153	protein N-linked glycosylation	124:153	Despite the controversy regarding the importance of protein N-linked glycosylation in species of the genus Plasmodium, genes potentially encoding core subunits of the oligosaccharyltransferase (OST) complex have already been characterized in completely sequenced genomes of malaria parasites.
30835280	2	48	from	present	523:529	arg1	species					545:551	Plasmodium species	534:551	Plasmodium species	534:551	Nevertheless, the currently established notion is that only four out of eight subunits of the OST complex-which is considered conserved across eukaryotes-are present in Plasmodium species.
30835280	7	49	theme	approach	1373:1380	arg1	necessity					1347:1355	the necessity	1343:1355	the necessity of a systematic approach	1343:1380	Thus, our work highlights the necessity of a systematic approach towards the characterization of OST subunits across eukaryotes.
30835280	5	50	theme	other	1116:1120	arg1	species					1122:1128	other species	1116:1128	other species	1116:1128	Interestingly, the main reason why the unusually short Ost4 subunit (36 residues long in yeast) has not been identified so far in plasmodia (and possibly other species) is the failure of gene-prediction pipelines to detect such a short coding sequence.
30835280	5	50	theme	other	1116:1120	arg1	plasmodia					1092:1100	plasmodia	1092:1100	plasmodia (and possibly other species)	1092:1129	Interestingly, the main reason why the unusually short Ost4 subunit (36 residues long in yeast) has not been identified so far in plasmodia (and possibly other species) is the failure of gene-prediction pipelines to detect such a short coding sequence.
30835280	0	51	theme	complex	49:55	arg1	view					11:14	An updated view	0:14	An updated view of the oligosaccharyltransferase complex in Plasmodium	0:69	An updated view of the oligosaccharyltransferase complex in Plasmodium.
30835280	5	52	theme	pipelines	1165:1173	arg1	reason					986:991	the main reason	977:991	the main reason why the unusually short Ost4 subunit (36 residues long in yeast) has not been identified so far in plasmodia (and possibly other species)	977:1129	Interestingly, the main reason why the unusually short Ost4 subunit (36 residues long in yeast) has not been identified so far in plasmodia (and possibly other species) is the failure of gene-prediction pipelines to detect such a short coding sequence.
30835280	5	52	theme	pipelines	1165:1173	arg1	failure					1138:1144	the failure	1134:1144	the failure of gene-prediction pipelines to detect such a short coding sequence	1134:1212	Interestingly, the main reason why the unusually short Ost4 subunit (36 residues long in yeast) has not been identified so far in plasmodia (and possibly other species) is the failure of gene-prediction pipelines to detect such a short coding sequence.
30835280	4	53	theme	existing	930:937	arg1	sequences					912:920	uncharacterized protein sequences	888:920	uncharacterized protein sequences already existing in sequence databases	888:959	In fact, most of the subunits currently considered as absent from Plasmodium refer to uncharacterized protein sequences already existing in sequence databases.
30835280	2	54	theme	complex-which	463:475	arg1	subunits					443:450	only four out of eight subunits	420:450	only four out of eight subunits of the OST complex-which	420:475	Nevertheless, the currently established notion is that only four out of eight subunits of the OST complex-which is considered conserved across eukaryotes-are present in Plasmodium species.
30835280	1	55	theme	protein	124:130	arg1	glycosylation					141:153	protein N-linked glycosylation	124:153	protein N-linked glycosylation	124:153	Despite the controversy regarding the importance of protein N-linked glycosylation in species of the genus Plasmodium, genes potentially encoding core subunits of the oligosaccharyltransferase (OST) complex have already been characterized in completely sequenced genomes of malaria parasites.
30835280	8	56	theme	eukaryotic	1584:1593	arg1	pathogens					1595:1603	eukaryotic pathogens	1584:1603	eukaryotic pathogens	1584:1603	This is necessary both for obtaining a concrete picture of the evolution of the OST complex but also for elucidating its possible role in eukaryotic pathogens.
30835280	1	57	theme	N-linked	132:139	arg1	glycosylation					141:153	protein N-linked glycosylation	124:153	protein N-linked glycosylation	124:153	Despite the controversy regarding the importance of protein N-linked glycosylation in species of the genus Plasmodium, genes potentially encoding core subunits of the oligosaccharyltransferase (OST) complex have already been characterized in completely sequenced genomes of malaria parasites.
30835280	1	58	from	importance	110:119	arg1	species					158:164	species	158:164	species of the genus Plasmodium	158:188	Despite the controversy regarding the importance of protein N-linked glycosylation in species of the genus Plasmodium, genes potentially encoding core subunits of the oligosaccharyltransferase (OST) complex have already been characterized in completely sequenced genomes of malaria parasites.
30835280	4	59	theme	sequence	942:949	arg1	databases					951:959	sequence databases	942:959	sequence databases	942:959	In fact, most of the subunits currently considered as absent from Plasmodium refer to uncharacterized protein sequences already existing in sequence databases.
30835280	2	60	dep	notion	405:410	arg1	subunits					443:450	only four out of eight subunits	420:450	only four out of eight subunits of the OST complex-which	420:475	Nevertheless, the currently established notion is that only four out of eight subunits of the OST complex-which is considered conserved across eukaryotes-are present in Plasmodium species.
30835280	1	61	theme	glycosylation	141:153	arg1	importance					110:119	the importance	106:119	the importance of protein N-linked glycosylation in species of the genus Plasmodium	106:188	Despite the controversy regarding the importance of protein N-linked glycosylation in species of the genus Plasmodium, genes potentially encoding core subunits of the oligosaccharyltransferase (OST) complex have already been characterized in completely sequenced genomes of malaria parasites.
30835280	4	62	from	existing	930:937	arg1	databases					951:959	sequence databases	942:959	sequence databases	942:959	In fact, most of the subunits currently considered as absent from Plasmodium refer to uncharacterized protein sequences already existing in sequence databases.
30835280	2	63	theme	established	393:403	arg1	notion					405:410	the currently established notion	379:410	the currently established notion is that only four out of eight subunits of the OST complex-which	379:475	Nevertheless, the currently established notion is that only four out of eight subunits of the OST complex-which is considered conserved across eukaryotes-are present in Plasmodium species.
30835280	6	64	with	species	1274:1280	arg1	genomes					1308:1314	completely sequenced genomes	1287:1314	completely sequenced genomes	1287:1314	We further identify elusive OST subunits in select protist species with completely sequenced genomes.
30835280	1	65	theme	oligosaccharyltransferase	239:263	arg1	complex					271:277	the oligosaccharyltransferase (OST) complex	235:277	the oligosaccharyltransferase (OST) complex	235:277	Despite the controversy regarding the importance of protein N-linked glycosylation in species of the genus Plasmodium, genes potentially encoding core subunits of the oligosaccharyltransferase (OST) complex have already been characterized in completely sequenced genomes of malaria parasites.
30835280	1	66	theme	malaria	346:352	arg1	parasites					354:362	malaria parasites	346:362	malaria parasites	346:362	Despite the controversy regarding the importance of protein N-linked glycosylation in species of the genus Plasmodium, genes potentially encoding core subunits of the oligosaccharyltransferase (OST) complex have already been characterized in completely sequenced genomes of malaria parasites.
30835280	5	67	theme	long	1043:1046	arg1	residues					1034:1041	36 residues	1031:1041	36 residues long in yeast	1031:1055	Interestingly, the main reason why the unusually short Ost4 subunit (36 residues long in yeast) has not been identified so far in plasmodia (and possibly other species) is the failure of gene-prediction pipelines to detect such a short coding sequence.
30835280	2	68	theme	OST	459:461	arg1	complex-which					463:475	the OST complex-which	455:475	the OST complex-which	455:475	Nevertheless, the currently established notion is that only four out of eight subunits of the OST complex-which is considered conserved across eukaryotes-are present in Plasmodium species.
30835280	5	69	theme	gene-prediction	1149:1163	arg1	pipelines					1165:1173	gene-prediction pipelines	1149:1173	gene-prediction pipelines	1149:1173	Interestingly, the main reason why the unusually short Ost4 subunit (36 residues long in yeast) has not been identified so far in plasmodia (and possibly other species) is the failure of gene-prediction pipelines to detect such a short coding sequence.
29720943	7	0	theme	increased	1276:1284	arg1	molecule					1309:1316	increased intercellular adhesion molecule 1	1276:1318	increased intercellular adhesion molecule 1 (ICAM-1)	1276:1327	Results: HG upregulated OGT mRNA and protein expression and protein O-GlcNAcylation levels (RL2 antibody) in HAECs, and showed increased intercellular adhesion molecule 1 (ICAM-1), vascular cell adhesion molecule 1 (VCAM-1), and E-selectin gene expression; ICAM-1 expression; and THP-1 adhesion.
29720943	7	0	theme	increased	1276:1284	arg1	ICAM-1					1321:1326	ICAM-1	1321:1326	ICAM-1	1321:1326	Results: HG upregulated OGT mRNA and protein expression and protein O-GlcNAcylation levels (RL2 antibody) in HAECs, and showed increased intercellular adhesion molecule 1 (ICAM-1), vascular cell adhesion molecule 1 (VCAM-1), and E-selectin gene expression; ICAM-1 expression; and THP-1 adhesion.
29720943	8	1	theme	family	1651:1656	arg1	members					1632:1638	members	1632:1638	members of miR-200 family	1632:1656	Bioinformatics analysis revealed homologous sequences between members of the miR-200 family and the 3'-untranslated region (3'-UTR) of OGT mRNA, and real-time PCR analysis confirmed that members of miR-200 family were significantly decreased in HG-stimulated HAECs.
29720943	7	2	theme	mRNA	1177:1180	arg1	expression					1194:1203	OGT mRNA and protein expression	1173:1203	OGT mRNA and protein expression	1173:1203	Results: HG upregulated OGT mRNA and protein expression and protein O-GlcNAcylation levels (RL2 antibody) in HAECs, and showed increased intercellular adhesion molecule 1 (ICAM-1), vascular cell adhesion molecule 1 (VCAM-1), and E-selectin gene expression; ICAM-1 expression; and THP-1 adhesion.
29720943	14	3	theme	endothelial	2697:2707	arg1	inflammation					2709:2720	HG-induced endothelial inflammation	2686:2720	HG-induced endothelial inflammation	2686:2720	Conclusion: miR-200a/200b are involved in modulating HG-induced endothelial inflammation by regulating OGT-mediated protein O-GlcNAcylation, suggesting the therapeutic role of miR-200a/200b on vascular complications in diabetes.
29720943	2	4	theme	oxidative	322:330	arg1	stress					332:337	oxidative stress	322:337	oxidative stress	322:337	Furthermore, oxidative stress promotes endothelial inflammation during diabetes.
29720943	1	5	theme	proteins	227:234	arg1	modification					211:222	Increased O-linked N-acetylglucosamine (O-GlcNAc) modification	161:222	Increased O-linked N-acetylglucosamine (O-GlcNAc) modification of proteins by O-GlcNAc transferase (OGT)	161:264	Background and Aims: Increased O-linked N-acetylglucosamine (O-GlcNAc) modification of proteins by O-GlcNAc transferase (OGT) is associated with diabetic complications.
29720943	4	6	theme	aortic	610:615	arg1	HAECs					636:640	HAECs	636:640	HAECs	636:640	In this study, we examined whether miR-200a and miR-200b regulate high-glucose (HG)-induced OGT expression in human aortic endothelial cells (HAECs) and whether miRNA-200a/200b downregulate OGT expression to control HG-induced endothelial inflammation.
29720943	4	6	theme	aortic	610:615	arg1	cells					629:633	human aortic endothelial cells	604:633	human aortic endothelial cells (HAECs)	604:641	In this study, we examined whether miR-200a and miR-200b regulate high-glucose (HG)-induced OGT expression in human aortic endothelial cells (HAECs) and whether miRNA-200a/200b downregulate OGT expression to control HG-induced endothelial inflammation.
29720943	13	7	theme	OGT	2592:2594	arg1	expression					2607:2616	endothelial OGT and ICAM-1 expression	2580:2616	endothelial OGT and ICAM-1 expression in db/db mice	2580:2630	These results were validated in vivo: tail-vein injection of miR-200a/200b mimics downregulated endothelial OGT and ICAM-1 expression in db/db mice.
29720943	12	8	theme	OGT	2224:2226	arg1	depletion					2228:2236	siRNA-mediated OGT depletion	2209:2236	siRNA-mediated OGT depletion	2209:2236	Additionally, siRNA-mediated OGT depletion reduced HG-induced protein O-GlcNAcylation; ICAM-1, VCAM-1, and E-selectin gene expression; ICAM-1 expression; and THP-1 adhesion, confirming that HG-induced endothelial inflammation is partially mediated via OGT-induced protein O-GlcNAcylation.
29720943	14	9	from	role	2801:2804	arg1	complications					2835:2847	vascular complications	2826:2847	vascular complications in diabetes	2826:2859	Conclusion: miR-200a/200b are involved in modulating HG-induced endothelial inflammation by regulating OGT-mediated protein O-GlcNAcylation, suggesting the therapeutic role of miR-200a/200b on vascular complications in diabetes.
29720943	1	10	theme	O-GlcNAc	239:246	arg1	OGT					261:263	OGT	261:263	OGT	261:263	Background and Aims: Increased O-linked N-acetylglucosamine (O-GlcNAc) modification of proteins by O-GlcNAc transferase (OGT) is associated with diabetic complications.
29720943	1	10	theme	O-GlcNAc	239:246	arg1	transferase					248:258	O-GlcNAc transferase	239:258	O-GlcNAc transferase (OGT)	239:264	Background and Aims: Increased O-linked N-acetylglucosamine (O-GlcNAc) modification of proteins by O-GlcNAc transferase (OGT) is associated with diabetic complications.
29720943	14	11	theme	miR-200a/200b	2809:2821	arg1	role					2801:2804	the therapeutic role	2785:2804	the therapeutic role of miR-200a/200b on vascular complications in diabetes	2785:2859	Conclusion: miR-200a/200b are involved in modulating HG-induced endothelial inflammation by regulating OGT-mediated protein O-GlcNAcylation, suggesting the therapeutic role of miR-200a/200b on vascular complications in diabetes.
29720943	11	12	theme	gene	2139:2142	arg1	expression					2144:2153	E-selectin gene expression	2128:2153	E-selectin gene expression	2128:2153	Transfection with miR-200a/200b mimics significantly inhibited HG-induced OGT mRNA expression, OGT protein expression; protein O-GlcNAcylation levels; ICAM-1, VCAM-1, and E-selectin gene expression; ICAM-1 expression; and THP-1 adhesion.
29720943	5	13	theme	high	783:786	arg1	glucose					788:794	high glucose	783:794	high glucose (25 mM)	783:802	Methods: HAECs were stimulated with high glucose (25 mM) for 12 and 24 h. Real-time polymerase chain reaction (PCR), western blotting, THP-1 adhesion assay, bioinformatics predication, transfection of miR-200a/200b mimic or inhibitor, luciferase reporter assay, and transfection of siRNA OGT were performed.
29720943	5	13	theme	high	783:786	arg1	mM					800:801	25 mM	797:801	25 mM	797:801	Methods: HAECs were stimulated with high glucose (25 mM) for 12 and 24 h. Real-time polymerase chain reaction (PCR), western blotting, THP-1 adhesion assay, bioinformatics predication, transfection of miR-200a/200b mimic or inhibitor, luciferase reporter assay, and transfection of siRNA OGT were performed.
29720943	14	14	theme	vascular	2826:2833	arg1	complications					2835:2847	vascular complications	2826:2847	vascular complications in diabetes	2826:2859	Conclusion: miR-200a/200b are involved in modulating HG-induced endothelial inflammation by regulating OGT-mediated protein O-GlcNAcylation, suggesting the therapeutic role of miR-200a/200b on vascular complications in diabetes.
29720943	4	15	theme	HG-induced	710:719	arg1	inflammation					733:744	HG-induced endothelial inflammation	710:744	HG-induced endothelial inflammation	710:744	In this study, we examined whether miR-200a and miR-200b regulate high-glucose (HG)-induced OGT expression in human aortic endothelial cells (HAECs) and whether miRNA-200a/200b downregulate OGT expression to control HG-induced endothelial inflammation.
29720943	7	16	theme	protein	1209:1215	arg1	levels					1233:1238	protein O-GlcNAcylation levels	1209:1238	protein O-GlcNAcylation levels (RL2 antibody)	1209:1253	Results: HG upregulated OGT mRNA and protein expression and protein O-GlcNAcylation levels (RL2 antibody) in HAECs, and showed increased intercellular adhesion molecule 1 (ICAM-1), vascular cell adhesion molecule 1 (VCAM-1), and E-selectin gene expression; ICAM-1 expression; and THP-1 adhesion.
29720943	7	16	theme	protein	1209:1215	arg1	antibody					1245:1252	RL2 antibody	1241:1252	RL2 antibody	1241:1252	Results: HG upregulated OGT mRNA and protein expression and protein O-GlcNAcylation levels (RL2 antibody) in HAECs, and showed increased intercellular adhesion molecule 1 (ICAM-1), vascular cell adhesion molecule 1 (VCAM-1), and E-selectin gene expression; ICAM-1 expression; and THP-1 adhesion.
29720943	5	17	theme	THP-1	882:886	arg1	assay					897:901	THP-1 adhesion assay	882:901	THP-1 adhesion assay	882:901	Methods: HAECs were stimulated with high glucose (25 mM) for 12 and 24 h. Real-time polymerase chain reaction (PCR), western blotting, THP-1 adhesion assay, bioinformatics predication, transfection of miR-200a/200b mimic or inhibitor, luciferase reporter assay, and transfection of siRNA OGT were performed.
29720943	9	18	theme	feedback	1753:1760	arg1	restraint					1762:1770	an impaired feedback restraint	1741:1770	an impaired feedback restraint	1741:1770	This suggests the presence of an impaired feedback restraint on HG-induced endothelial protein O-GlcNAcylation levels because of OGT upregulation.
29720943	7	19	theme	gene	1389:1392	arg1	expression					1394:1403	E-selectin gene expression	1378:1403	E-selectin gene expression	1378:1403	Results: HG upregulated OGT mRNA and protein expression and protein O-GlcNAcylation levels (RL2 antibody) in HAECs, and showed increased intercellular adhesion molecule 1 (ICAM-1), vascular cell adhesion molecule 1 (VCAM-1), and E-selectin gene expression; ICAM-1 expression; and THP-1 adhesion.
29720943	11	20	with	Transfection	1957:1968	arg1	miR-200a/200b					1975:1987	miR-200a/200b	1975:1987	miR-200a/200b	1975:1987	Transfection with miR-200a/200b mimics significantly inhibited HG-induced OGT mRNA expression, OGT protein expression; protein O-GlcNAcylation levels; ICAM-1, VCAM-1, and E-selectin gene expression; ICAM-1 expression; and THP-1 adhesion.
29720943	11	21	theme	mRNA	2035:2038	arg1	expression					2040:2049	HG-induced OGT mRNA expression	2020:2049	HG-induced OGT mRNA expression	2020:2049	Transfection with miR-200a/200b mimics significantly inhibited HG-induced OGT mRNA expression, OGT protein expression; protein O-GlcNAcylation levels; ICAM-1, VCAM-1, and E-selectin gene expression; ICAM-1 expression; and THP-1 adhesion.
29720943	1	22	theme	Increased	161:169	arg1	modification					211:222	Increased O-linked N-acetylglucosamine (O-GlcNAc) modification	161:222	Increased O-linked N-acetylglucosamine (O-GlcNAc) modification of proteins by O-GlcNAc transferase (OGT)	161:264	Background and Aims: Increased O-linked N-acetylglucosamine (O-GlcNAc) modification of proteins by O-GlcNAc transferase (OGT) is associated with diabetic complications.
29720943	12	23	theme	HG-induced	2385:2394	arg1	inflammation					2408:2419	HG-induced endothelial inflammation	2385:2419	HG-induced endothelial inflammation	2385:2419	Additionally, siRNA-mediated OGT depletion reduced HG-induced protein O-GlcNAcylation; ICAM-1, VCAM-1, and E-selectin gene expression; ICAM-1 expression; and THP-1 adhesion, confirming that HG-induced endothelial inflammation is partially mediated via OGT-induced protein O-GlcNAcylation.
29720943	13	24	theme	tail-vein	2522:2530	arg1	injection					2532:2540	tail-vein injection	2522:2540	tail-vein injection of miR-200a/200b mimics	2522:2564	These results were validated in vivo: tail-vein injection of miR-200a/200b mimics downregulated endothelial OGT and ICAM-1 expression in db/db mice.
29720943	13	25	theme	ICAM-1	2600:2605	arg1	expression					2607:2616	endothelial OGT and ICAM-1 expression	2580:2616	endothelial OGT and ICAM-1 expression in db/db mice	2580:2630	These results were validated in vivo: tail-vein injection of miR-200a/200b mimics downregulated endothelial OGT and ICAM-1 expression in db/db mice.
29720943	5	26	theme	inhibitor	971:979	arg1	reaction					848:855	Real-time polymerase chain reaction	821:855	Real-time polymerase chain reaction (PCR)	821:861	Methods: HAECs were stimulated with high glucose (25 mM) for 12 and 24 h. Real-time polymerase chain reaction (PCR), western blotting, THP-1 adhesion assay, bioinformatics predication, transfection of miR-200a/200b mimic or inhibitor, luciferase reporter assay, and transfection of siRNA OGT were performed.
29720943	5	26	theme	inhibitor	971:979	arg1	blotting					872:879	western blotting	864:879	western blotting	864:879	Methods: HAECs were stimulated with high glucose (25 mM) for 12 and 24 h. Real-time polymerase chain reaction (PCR), western blotting, THP-1 adhesion assay, bioinformatics predication, transfection of miR-200a/200b mimic or inhibitor, luciferase reporter assay, and transfection of siRNA OGT were performed.
29720943	5	26	theme	inhibitor	971:979	arg1	predication					919:929	bioinformatics predication	904:929	bioinformatics predication	904:929	Methods: HAECs were stimulated with high glucose (25 mM) for 12 and 24 h. Real-time polymerase chain reaction (PCR), western blotting, THP-1 adhesion assay, bioinformatics predication, transfection of miR-200a/200b mimic or inhibitor, luciferase reporter assay, and transfection of siRNA OGT were performed.
29720943	5	26	theme	inhibitor	971:979	arg1	transfection					1013:1024	transfection	1013:1024	transfection of siRNA OGT	1013:1037	Methods: HAECs were stimulated with high glucose (25 mM) for 12 and 24 h. Real-time polymerase chain reaction (PCR), western blotting, THP-1 adhesion assay, bioinformatics predication, transfection of miR-200a/200b mimic or inhibitor, luciferase reporter assay, and transfection of siRNA OGT were performed.
29720943	5	26	theme	inhibitor	971:979	arg1	transfection					932:943	transfection	932:943	transfection of miR-200a/200b mimic or inhibitor	932:979	Methods: HAECs were stimulated with high glucose (25 mM) for 12 and 24 h. Real-time polymerase chain reaction (PCR), western blotting, THP-1 adhesion assay, bioinformatics predication, transfection of miR-200a/200b mimic or inhibitor, luciferase reporter assay, and transfection of siRNA OGT were performed.
29720943	5	26	theme	inhibitor	971:979	arg1	PCR					858:860	PCR	858:860	PCR	858:860	Methods: HAECs were stimulated with high glucose (25 mM) for 12 and 24 h. Real-time polymerase chain reaction (PCR), western blotting, THP-1 adhesion assay, bioinformatics predication, transfection of miR-200a/200b mimic or inhibitor, luciferase reporter assay, and transfection of siRNA OGT were performed.
29720943	5	26	theme	inhibitor	971:979	arg1	assay					897:901	THP-1 adhesion assay	882:901	THP-1 adhesion assay	882:901	Methods: HAECs were stimulated with high glucose (25 mM) for 12 and 24 h. Real-time polymerase chain reaction (PCR), western blotting, THP-1 adhesion assay, bioinformatics predication, transfection of miR-200a/200b mimic or inhibitor, luciferase reporter assay, and transfection of siRNA OGT were performed.
29720943	5	26	theme	inhibitor	971:979	arg1	assay					1002:1006	luciferase reporter assay	982:1006	luciferase reporter assay	982:1006	Methods: HAECs were stimulated with high glucose (25 mM) for 12 and 24 h. Real-time polymerase chain reaction (PCR), western blotting, THP-1 adhesion assay, bioinformatics predication, transfection of miR-200a/200b mimic or inhibitor, luciferase reporter assay, and transfection of siRNA OGT were performed.
29720943	6	27	theme	aortic	1059:1064	arg1	endothelium					1066:1076	The aortic endothelium	1055:1076	The aortic endothelium of db/db diabetic mice	1055:1099	The aortic endothelium of db/db diabetic mice was evaluated by immunohistochemistry staining.
29720943	9	28	theme	endothelial	1786:1796	arg1	levels					1822:1827	HG-induced endothelial protein O-GlcNAcylation levels	1775:1827	HG-induced endothelial protein O-GlcNAcylation levels	1775:1827	This suggests the presence of an impaired feedback restraint on HG-induced endothelial protein O-GlcNAcylation levels because of OGT upregulation.
29720943	7	29	theme	cell	1339:1342	arg1	molecule					1353:1360	vascular cell adhesion molecule 1	1330:1362	vascular cell adhesion molecule 1 (VCAM-1)	1330:1371	Results: HG upregulated OGT mRNA and protein expression and protein O-GlcNAcylation levels (RL2 antibody) in HAECs, and showed increased intercellular adhesion molecule 1 (ICAM-1), vascular cell adhesion molecule 1 (VCAM-1), and E-selectin gene expression; ICAM-1 expression; and THP-1 adhesion.
29720943	7	29	theme	cell	1339:1342	arg1	VCAM-1					1365:1370	VCAM-1	1365:1370	VCAM-1	1365:1370	Results: HG upregulated OGT mRNA and protein expression and protein O-GlcNAcylation levels (RL2 antibody) in HAECs, and showed increased intercellular adhesion molecule 1 (ICAM-1), vascular cell adhesion molecule 1 (VCAM-1), and E-selectin gene expression; ICAM-1 expression; and THP-1 adhesion.
29720943	0	30	theme	O-linked	87:94	arg1	Transferase					116:126	O-linked N-Acetylglucosamine Transferase	87:126	O-linked N-Acetylglucosamine Transferase Expression	87:137	MicroRNA-200a/200b Modulate High Glucose-Induced Endothelial Inflammation by Targeting O-linked N-Acetylglucosamine Transferase Expression.
29720943	7	31	theme	O-GlcNAcylation	1217:1231	arg1	levels					1233:1238	protein O-GlcNAcylation levels	1209:1238	protein O-GlcNAcylation levels (RL2 antibody)	1209:1253	Results: HG upregulated OGT mRNA and protein expression and protein O-GlcNAcylation levels (RL2 antibody) in HAECs, and showed increased intercellular adhesion molecule 1 (ICAM-1), vascular cell adhesion molecule 1 (VCAM-1), and E-selectin gene expression; ICAM-1 expression; and THP-1 adhesion.
29720943	7	31	theme	O-GlcNAcylation	1217:1231	arg1	antibody					1245:1252	RL2 antibody	1241:1252	RL2 antibody	1241:1252	Results: HG upregulated OGT mRNA and protein expression and protein O-GlcNAcylation levels (RL2 antibody) in HAECs, and showed increased intercellular adhesion molecule 1 (ICAM-1), vascular cell adhesion molecule 1 (VCAM-1), and E-selectin gene expression; ICAM-1 expression; and THP-1 adhesion.
29720943	9	32	theme	O-GlcNAcylation	1806:1820	arg1	levels					1822:1827	HG-induced endothelial protein O-GlcNAcylation levels	1775:1827	HG-induced endothelial protein O-GlcNAcylation levels	1775:1827	This suggests the presence of an impaired feedback restraint on HG-induced endothelial protein O-GlcNAcylation levels because of OGT upregulation.
29720943	8	33	theme	family	1530:1535	arg1	members					1507:1513	members	1507:1513	members of the miR-200 family	1507:1535	Bioinformatics analysis revealed homologous sequences between members of the miR-200 family and the 3'-untranslated region (3'-UTR) of OGT mRNA, and real-time PCR analysis confirmed that members of miR-200 family were significantly decreased in HG-stimulated HAECs.
29720943	11	34	theme	ICAM-1	2156:2161	arg1	expression					2163:2172	ICAM-1 expression	2156:2172	ICAM-1 expression	2156:2172	Transfection with miR-200a/200b mimics significantly inhibited HG-induced OGT mRNA expression, OGT protein expression; protein O-GlcNAcylation levels; ICAM-1, VCAM-1, and E-selectin gene expression; ICAM-1 expression; and THP-1 adhesion.
29720943	0	35	theme	Transferase	116:126	arg1	Expression					128:137	O-linked N-Acetylglucosamine Transferase Expression	87:137	O-linked N-Acetylglucosamine Transferase Expression	87:137	MicroRNA-200a/200b Modulate High Glucose-Induced Endothelial Inflammation by Targeting O-linked N-Acetylglucosamine Transferase Expression.
29720943	10	36	theme	luciferase	1860:1869	arg1	assay					1880:1884	A luciferase reporter assay	1858:1884	A luciferase reporter assay	1858:1884	A luciferase reporter assay demonstrated that miR-200a/200b mimics bind to the 3'-UTR of OGT mRNA.
29720943	13	37	theme	miR-200a/200b	2545:2557	arg1	mimics					2559:2564	miR-200a/200b mimics	2545:2564	miR-200a/200b mimics	2545:2564	These results were validated in vivo: tail-vein injection of miR-200a/200b mimics downregulated endothelial OGT and ICAM-1 expression in db/db mice.
29720943	1	38	theme	N-acetylglucosamine	180:198	arg1	modification					211:222	Increased O-linked N-acetylglucosamine (O-GlcNAc) modification	161:222	Increased O-linked N-acetylglucosamine (O-GlcNAc) modification of proteins by O-GlcNAc transferase (OGT)	161:264	Background and Aims: Increased O-linked N-acetylglucosamine (O-GlcNAc) modification of proteins by O-GlcNAc transferase (OGT) is associated with diabetic complications.
29720943	9	39	theme	OGT	1840:1842	arg1	upregulation					1844:1855	OGT upregulation	1840:1855	OGT upregulation	1840:1855	This suggests the presence of an impaired feedback restraint on HG-induced endothelial protein O-GlcNAcylation levels because of OGT upregulation.
29720943	6	40	theme	diabetic	1087:1094	arg1	mice					1096:1099	db/db diabetic mice	1081:1099	db/db diabetic mice	1081:1099	The aortic endothelium of db/db diabetic mice was evaluated by immunohistochemistry staining.
29720943	5	41	dep	miR-200a/200b	948:960	arg1	mimic					962:966	mimic	962:966	mimic	962:966	Methods: HAECs were stimulated with high glucose (25 mM) for 12 and 24 h. Real-time polymerase chain reaction (PCR), western blotting, THP-1 adhesion assay, bioinformatics predication, transfection of miR-200a/200b mimic or inhibitor, luciferase reporter assay, and transfection of siRNA OGT were performed.
29720943	14	42	theme	protein	2749:2755	arg1	O-GlcNAcylation					2757:2771	OGT-mediated protein O-GlcNAcylation	2736:2771	OGT-mediated protein O-GlcNAcylation	2736:2771	Conclusion: miR-200a/200b are involved in modulating HG-induced endothelial inflammation by regulating OGT-mediated protein O-GlcNAcylation, suggesting the therapeutic role of miR-200a/200b on vascular complications in diabetes.
29720943	10	43	theme	mRNA	1951:1954	arg1	3'-UTR					1937:1942	the 3'-UTR	1933:1942	the 3'-UTR of OGT mRNA	1933:1954	A luciferase reporter assay demonstrated that miR-200a/200b mimics bind to the 3'-UTR of OGT mRNA.
29720943	4	44	theme	endothelial	617:627	arg1	HAECs					636:640	HAECs	636:640	HAECs	636:640	In this study, we examined whether miR-200a and miR-200b regulate high-glucose (HG)-induced OGT expression in human aortic endothelial cells (HAECs) and whether miRNA-200a/200b downregulate OGT expression to control HG-induced endothelial inflammation.
29720943	4	44	theme	endothelial	617:627	arg1	cells					629:633	human aortic endothelial cells	604:633	human aortic endothelial cells (HAECs)	604:641	In this study, we examined whether miR-200a and miR-200b regulate high-glucose (HG)-induced OGT expression in human aortic endothelial cells (HAECs) and whether miRNA-200a/200b downregulate OGT expression to control HG-induced endothelial inflammation.
29720943	5	45	theme	luciferase	982:991	arg1	assay					1002:1006	luciferase reporter assay	982:1006	luciferase reporter assay	982:1006	Methods: HAECs were stimulated with high glucose (25 mM) for 12 and 24 h. Real-time polymerase chain reaction (PCR), western blotting, THP-1 adhesion assay, bioinformatics predication, transfection of miR-200a/200b mimic or inhibitor, luciferase reporter assay, and transfection of siRNA OGT were performed.
29720943	4	46	theme	human	604:608	arg1	HAECs					636:640	HAECs	636:640	HAECs	636:640	In this study, we examined whether miR-200a and miR-200b regulate high-glucose (HG)-induced OGT expression in human aortic endothelial cells (HAECs) and whether miRNA-200a/200b downregulate OGT expression to control HG-induced endothelial inflammation.
29720943	4	46	theme	human	604:608	arg1	cells					629:633	human aortic endothelial cells	604:633	human aortic endothelial cells (HAECs)	604:641	In this study, we examined whether miR-200a and miR-200b regulate high-glucose (HG)-induced OGT expression in human aortic endothelial cells (HAECs) and whether miRNA-200a/200b downregulate OGT expression to control HG-induced endothelial inflammation.
29720943	12	47	theme	E-selectin	2302:2311	arg1	expression					2318:2327	ICAM-1, VCAM-1, and E-selectin gene expression	2282:2327	ICAM-1, VCAM-1, and E-selectin gene expression	2282:2327	Additionally, siRNA-mediated OGT depletion reduced HG-induced protein O-GlcNAcylation; ICAM-1, VCAM-1, and E-selectin gene expression; ICAM-1 expression; and THP-1 adhesion, confirming that HG-induced endothelial inflammation is partially mediated via OGT-induced protein O-GlcNAcylation.
29720943	0	48	theme	High	28:31	arg1	Inflammation					61:72	High Glucose-Induced Endothelial Inflammation	28:72	High Glucose-Induced Endothelial Inflammation	28:72	MicroRNA-200a/200b Modulate High Glucose-Induced Endothelial Inflammation by Targeting O-linked N-Acetylglucosamine Transferase Expression.
29720943	7	49	theme	adhesion	1344:1351	arg1	molecule					1353:1360	vascular cell adhesion molecule 1	1330:1362	vascular cell adhesion molecule 1 (VCAM-1)	1330:1371	Results: HG upregulated OGT mRNA and protein expression and protein O-GlcNAcylation levels (RL2 antibody) in HAECs, and showed increased intercellular adhesion molecule 1 (ICAM-1), vascular cell adhesion molecule 1 (VCAM-1), and E-selectin gene expression; ICAM-1 expression; and THP-1 adhesion.
29720943	7	49	theme	adhesion	1344:1351	arg1	VCAM-1					1365:1370	VCAM-1	1365:1370	VCAM-1	1365:1370	Results: HG upregulated OGT mRNA and protein expression and protein O-GlcNAcylation levels (RL2 antibody) in HAECs, and showed increased intercellular adhesion molecule 1 (ICAM-1), vascular cell adhesion molecule 1 (VCAM-1), and E-selectin gene expression; ICAM-1 expression; and THP-1 adhesion.
29720943	3	50	theme	oxidative	476:484	arg1	stress					486:491	oxidative stress	476:491	oxidative stress	476:491	A previous study reported that microRNA-200 (miR-200) family members are sensitive to oxidative stress.
29720943	1	51	mod	modification	211:222	arg3	O-GlcNAc					201:208	O-GlcNAc	201:208	O-GlcNAc	201:208	Background and Aims: Increased O-linked N-acetylglucosamine (O-GlcNAc) modification of proteins by O-GlcNAc transferase (OGT) is associated with diabetic complications.
29720943	1	51	mod	modification	211:222	arg1	proteins					227:234	proteins	227:234	proteins	227:234	Background and Aims: Increased O-linked N-acetylglucosamine (O-GlcNAc) modification of proteins by O-GlcNAc transferase (OGT) is associated with diabetic complications.
29720943	1	51	mod	modification	211:222	arg3	N-acetylglucosamine					180:198	O-linked N-acetylglucosamine	171:198	Increased O-linked N-acetylglucosamine (O-GlcNAc) modification of proteins by O-GlcNAc transferase (OGT)	161:264	Background and Aims: Increased O-linked N-acetylglucosamine (O-GlcNAc) modification of proteins by O-GlcNAc transferase (OGT) is associated with diabetic complications.
29720943	5	52	theme	polymerase	831:840	arg1	PCR					858:860	PCR	858:860	PCR	858:860	Methods: HAECs were stimulated with high glucose (25 mM) for 12 and 24 h. Real-time polymerase chain reaction (PCR), western blotting, THP-1 adhesion assay, bioinformatics predication, transfection of miR-200a/200b mimic or inhibitor, luciferase reporter assay, and transfection of siRNA OGT were performed.
29720943	5	52	theme	polymerase	831:840	arg1	reaction					848:855	Real-time polymerase chain reaction	821:855	Real-time polymerase chain reaction (PCR)	821:861	Methods: HAECs were stimulated with high glucose (25 mM) for 12 and 24 h. Real-time polymerase chain reaction (PCR), western blotting, THP-1 adhesion assay, bioinformatics predication, transfection of miR-200a/200b mimic or inhibitor, luciferase reporter assay, and transfection of siRNA OGT were performed.
29720943	0	53	theme	Endothelial	49:59	arg1	Inflammation					61:72	High Glucose-Induced Endothelial Inflammation	28:72	High Glucose-Induced Endothelial Inflammation	28:72	MicroRNA-200a/200b Modulate High Glucose-Induced Endothelial Inflammation by Targeting O-linked N-Acetylglucosamine Transferase Expression.
29720943	7	54	theme	vascular	1330:1337	arg1	molecule					1353:1360	vascular cell adhesion molecule 1	1330:1362	vascular cell adhesion molecule 1 (VCAM-1)	1330:1371	Results: HG upregulated OGT mRNA and protein expression and protein O-GlcNAcylation levels (RL2 antibody) in HAECs, and showed increased intercellular adhesion molecule 1 (ICAM-1), vascular cell adhesion molecule 1 (VCAM-1), and E-selectin gene expression; ICAM-1 expression; and THP-1 adhesion.
29720943	7	54	theme	vascular	1330:1337	arg1	VCAM-1					1365:1370	VCAM-1	1365:1370	VCAM-1	1365:1370	Results: HG upregulated OGT mRNA and protein expression and protein O-GlcNAcylation levels (RL2 antibody) in HAECs, and showed increased intercellular adhesion molecule 1 (ICAM-1), vascular cell adhesion molecule 1 (VCAM-1), and E-selectin gene expression; ICAM-1 expression; and THP-1 adhesion.
29720943	4	55	theme	OGT	586:588	arg1	expression					590:599	high-glucose (HG)-induced OGT expression	560:599	high-glucose (HG)-induced OGT expression in human aortic endothelial cells (HAECs)	560:641	In this study, we examined whether miR-200a and miR-200b regulate high-glucose (HG)-induced OGT expression in human aortic endothelial cells (HAECs) and whether miRNA-200a/200b downregulate OGT expression to control HG-induced endothelial inflammation.
29720943	1	56	link	O-linked	171:178	arg1	O-GlcNAc					201:208	O-GlcNAc	201:208	O-GlcNAc	201:208	Background and Aims: Increased O-linked N-acetylglucosamine (O-GlcNAc) modification of proteins by O-GlcNAc transferase (OGT) is associated with diabetic complications.
29720943	1	56	link	O-linked	171:178	arg1	N-acetylglucosamine					180:198	O-linked N-acetylglucosamine	171:198	Increased O-linked N-acetylglucosamine (O-GlcNAc) modification of proteins by O-GlcNAc transferase (OGT)	161:264	Background and Aims: Increased O-linked N-acetylglucosamine (O-GlcNAc) modification of proteins by O-GlcNAc transferase (OGT) is associated with diabetic complications.
29720943	7	57	theme	protein	1186:1192	arg1	expression					1194:1203	OGT mRNA and protein expression	1173:1203	OGT mRNA and protein expression	1173:1203	Results: HG upregulated OGT mRNA and protein expression and protein O-GlcNAcylation levels (RL2 antibody) in HAECs, and showed increased intercellular adhesion molecule 1 (ICAM-1), vascular cell adhesion molecule 1 (VCAM-1), and E-selectin gene expression; ICAM-1 expression; and THP-1 adhesion.
29720943	8	58	theme	PCR	1604:1606	arg1	analysis					1608:1615	real-time PCR analysis	1594:1615	real-time PCR analysis	1594:1615	Bioinformatics analysis revealed homologous sequences between members of the miR-200 family and the 3'-untranslated region (3'-UTR) of OGT mRNA, and real-time PCR analysis confirmed that members of miR-200 family were significantly decreased in HG-stimulated HAECs.
29720943	10	59	theme	OGT	1947:1949	arg1	mRNA					1951:1954	OGT mRNA	1947:1954	OGT mRNA	1947:1954	A luciferase reporter assay demonstrated that miR-200a/200b mimics bind to the 3'-UTR of OGT mRNA.
29720943	11	60	theme	O-GlcNAcylation	2084:2098	arg1	levels					2100:2105	protein O-GlcNAcylation levels	2076:2105	protein O-GlcNAcylation levels	2076:2105	Transfection with miR-200a/200b mimics significantly inhibited HG-induced OGT mRNA expression, OGT protein expression; protein O-GlcNAcylation levels; ICAM-1, VCAM-1, and E-selectin gene expression; ICAM-1 expression; and THP-1 adhesion.
29720943	5	61	theme	OGT	1035:1037	arg1	reaction					848:855	Real-time polymerase chain reaction	821:855	Real-time polymerase chain reaction (PCR)	821:861	Methods: HAECs were stimulated with high glucose (25 mM) for 12 and 24 h. Real-time polymerase chain reaction (PCR), western blotting, THP-1 adhesion assay, bioinformatics predication, transfection of miR-200a/200b mimic or inhibitor, luciferase reporter assay, and transfection of siRNA OGT were performed.
29720943	5	61	theme	OGT	1035:1037	arg1	blotting					872:879	western blotting	864:879	western blotting	864:879	Methods: HAECs were stimulated with high glucose (25 mM) for 12 and 24 h. Real-time polymerase chain reaction (PCR), western blotting, THP-1 adhesion assay, bioinformatics predication, transfection of miR-200a/200b mimic or inhibitor, luciferase reporter assay, and transfection of siRNA OGT were performed.
29720943	5	61	theme	OGT	1035:1037	arg1	predication					919:929	bioinformatics predication	904:929	bioinformatics predication	904:929	Methods: HAECs were stimulated with high glucose (25 mM) for 12 and 24 h. Real-time polymerase chain reaction (PCR), western blotting, THP-1 adhesion assay, bioinformatics predication, transfection of miR-200a/200b mimic or inhibitor, luciferase reporter assay, and transfection of siRNA OGT were performed.
29720943	5	61	theme	OGT	1035:1037	arg1	transfection					1013:1024	transfection	1013:1024	transfection of siRNA OGT	1013:1037	Methods: HAECs were stimulated with high glucose (25 mM) for 12 and 24 h. Real-time polymerase chain reaction (PCR), western blotting, THP-1 adhesion assay, bioinformatics predication, transfection of miR-200a/200b mimic or inhibitor, luciferase reporter assay, and transfection of siRNA OGT were performed.
29720943	5	61	theme	OGT	1035:1037	arg1	transfection					932:943	transfection	932:943	transfection of miR-200a/200b mimic or inhibitor	932:979	Methods: HAECs were stimulated with high glucose (25 mM) for 12 and 24 h. Real-time polymerase chain reaction (PCR), western blotting, THP-1 adhesion assay, bioinformatics predication, transfection of miR-200a/200b mimic or inhibitor, luciferase reporter assay, and transfection of siRNA OGT were performed.
29720943	5	61	theme	OGT	1035:1037	arg1	PCR					858:860	PCR	858:860	PCR	858:860	Methods: HAECs were stimulated with high glucose (25 mM) for 12 and 24 h. Real-time polymerase chain reaction (PCR), western blotting, THP-1 adhesion assay, bioinformatics predication, transfection of miR-200a/200b mimic or inhibitor, luciferase reporter assay, and transfection of siRNA OGT were performed.
29720943	5	61	theme	OGT	1035:1037	arg1	assay					897:901	THP-1 adhesion assay	882:901	THP-1 adhesion assay	882:901	Methods: HAECs were stimulated with high glucose (25 mM) for 12 and 24 h. Real-time polymerase chain reaction (PCR), western blotting, THP-1 adhesion assay, bioinformatics predication, transfection of miR-200a/200b mimic or inhibitor, luciferase reporter assay, and transfection of siRNA OGT were performed.
29720943	5	61	theme	OGT	1035:1037	arg1	assay					1002:1006	luciferase reporter assay	982:1006	luciferase reporter assay	982:1006	Methods: HAECs were stimulated with high glucose (25 mM) for 12 and 24 h. Real-time polymerase chain reaction (PCR), western blotting, THP-1 adhesion assay, bioinformatics predication, transfection of miR-200a/200b mimic or inhibitor, luciferase reporter assay, and transfection of siRNA OGT were performed.
29720943	12	62	theme	protein	2257:2263	arg1	O-GlcNAcylation					2265:2279	HG-induced protein O-GlcNAcylation	2246:2279	HG-induced protein O-GlcNAcylation	2246:2279	Additionally, siRNA-mediated OGT depletion reduced HG-induced protein O-GlcNAcylation; ICAM-1, VCAM-1, and E-selectin gene expression; ICAM-1 expression; and THP-1 adhesion, confirming that HG-induced endothelial inflammation is partially mediated via OGT-induced protein O-GlcNAcylation.
29720943	8	63	theme	HG-stimulated	1690:1702	arg1	HAECs					1704:1708	HG-stimulated HAECs	1690:1708	HG-stimulated HAECs	1690:1708	Bioinformatics analysis revealed homologous sequences between members of the miR-200 family and the 3'-untranslated region (3'-UTR) of OGT mRNA, and real-time PCR analysis confirmed that members of miR-200 family were significantly decreased in HG-stimulated HAECs.
29720943	4	64	theme	-induced	577:584	arg1	expression					590:599	high-glucose (HG)-induced OGT expression	560:599	high-glucose (HG)-induced OGT expression in human aortic endothelial cells (HAECs)	560:641	In this study, we examined whether miR-200a and miR-200b regulate high-glucose (HG)-induced OGT expression in human aortic endothelial cells (HAECs) and whether miRNA-200a/200b downregulate OGT expression to control HG-induced endothelial inflammation.
29720943	7	65	theme	OGT	1173:1175	arg1	mRNA					1177:1180	OGT mRNA	1173:1180	OGT mRNA	1173:1180	Results: HG upregulated OGT mRNA and protein expression and protein O-GlcNAcylation levels (RL2 antibody) in HAECs, and showed increased intercellular adhesion molecule 1 (ICAM-1), vascular cell adhesion molecule 1 (VCAM-1), and E-selectin gene expression; ICAM-1 expression; and THP-1 adhesion.
29720943	7	66	theme	intercellular	1286:1298	arg1	molecule					1309:1316	increased intercellular adhesion molecule 1	1276:1318	increased intercellular adhesion molecule 1 (ICAM-1)	1276:1327	Results: HG upregulated OGT mRNA and protein expression and protein O-GlcNAcylation levels (RL2 antibody) in HAECs, and showed increased intercellular adhesion molecule 1 (ICAM-1), vascular cell adhesion molecule 1 (VCAM-1), and E-selectin gene expression; ICAM-1 expression; and THP-1 adhesion.
29720943	7	66	theme	intercellular	1286:1298	arg1	ICAM-1					1321:1326	ICAM-1	1321:1326	ICAM-1	1321:1326	Results: HG upregulated OGT mRNA and protein expression and protein O-GlcNAcylation levels (RL2 antibody) in HAECs, and showed increased intercellular adhesion molecule 1 (ICAM-1), vascular cell adhesion molecule 1 (VCAM-1), and E-selectin gene expression; ICAM-1 expression; and THP-1 adhesion.
29720943	12	67	theme	protein	2459:2465	arg1	O-GlcNAcylation					2467:2481	OGT-induced protein O-GlcNAcylation	2447:2481	OGT-induced protein O-GlcNAcylation	2447:2481	Additionally, siRNA-mediated OGT depletion reduced HG-induced protein O-GlcNAcylation; ICAM-1, VCAM-1, and E-selectin gene expression; ICAM-1 expression; and THP-1 adhesion, confirming that HG-induced endothelial inflammation is partially mediated via OGT-induced protein O-GlcNAcylation.
29720943	2	68	theme	endothelial	348:358	arg1	inflammation					360:371	endothelial inflammation	348:371	endothelial inflammation	348:371	Furthermore, oxidative stress promotes endothelial inflammation during diabetes.
29720943	11	69	theme	THP-1	2179:2183	arg1	adhesion					2185:2192	THP-1 adhesion	2179:2192	THP-1 adhesion	2179:2192	Transfection with miR-200a/200b mimics significantly inhibited HG-induced OGT mRNA expression, OGT protein expression; protein O-GlcNAcylation levels; ICAM-1, VCAM-1, and E-selectin gene expression; ICAM-1 expression; and THP-1 adhesion.
29720943	11	70	theme	protein	2076:2082	arg1	levels					2100:2105	protein O-GlcNAcylation levels	2076:2105	protein O-GlcNAcylation levels	2076:2105	Transfection with miR-200a/200b mimics significantly inhibited HG-induced OGT mRNA expression, OGT protein expression; protein O-GlcNAcylation levels; ICAM-1, VCAM-1, and E-selectin gene expression; ICAM-1 expression; and THP-1 adhesion.
29720943	5	71	theme	adhesion	888:895	arg1	assay					897:901	THP-1 adhesion assay	882:901	THP-1 adhesion assay	882:901	Methods: HAECs were stimulated with high glucose (25 mM) for 12 and 24 h. Real-time polymerase chain reaction (PCR), western blotting, THP-1 adhesion assay, bioinformatics predication, transfection of miR-200a/200b mimic or inhibitor, luciferase reporter assay, and transfection of siRNA OGT were performed.
29720943	11	72	theme	protein	2056:2062	arg1	expression					2064:2073	OGT protein expression	2052:2073	OGT protein expression	2052:2073	Transfection with miR-200a/200b mimics significantly inhibited HG-induced OGT mRNA expression, OGT protein expression; protein O-GlcNAcylation levels; ICAM-1, VCAM-1, and E-selectin gene expression; ICAM-1 expression; and THP-1 adhesion.
29720943	4	73	theme	endothelial	721:731	arg1	inflammation					733:744	HG-induced endothelial inflammation	710:744	HG-induced endothelial inflammation	710:744	In this study, we examined whether miR-200a and miR-200b regulate high-glucose (HG)-induced OGT expression in human aortic endothelial cells (HAECs) and whether miRNA-200a/200b downregulate OGT expression to control HG-induced endothelial inflammation.
29720943	8	74	theme	3'-untranslated	1545:1559	arg1	3'-UTR					1569:1574	3'-UTR	1569:1574	3'-UTR	1569:1574	Bioinformatics analysis revealed homologous sequences between members of the miR-200 family and the 3'-untranslated region (3'-UTR) of OGT mRNA, and real-time PCR analysis confirmed that members of miR-200 family were significantly decreased in HG-stimulated HAECs.
29720943	8	74	theme	3'-untranslated	1545:1559	arg1	region					1561:1566	the 3'-untranslated region	1541:1566	the 3'-untranslated region (3'-UTR) of OGT mRNA	1541:1587	Bioinformatics analysis revealed homologous sequences between members of the miR-200 family and the 3'-untranslated region (3'-UTR) of OGT mRNA, and real-time PCR analysis confirmed that members of miR-200 family were significantly decreased in HG-stimulated HAECs.
29720943	4	75	from	expression	590:599	arg1	HAECs					636:640	HAECs	636:640	HAECs	636:640	In this study, we examined whether miR-200a and miR-200b regulate high-glucose (HG)-induced OGT expression in human aortic endothelial cells (HAECs) and whether miRNA-200a/200b downregulate OGT expression to control HG-induced endothelial inflammation.
29720943	4	75	from	expression	590:599	arg1	cells					629:633	human aortic endothelial cells	604:633	human aortic endothelial cells (HAECs)	604:641	In this study, we examined whether miR-200a and miR-200b regulate high-glucose (HG)-induced OGT expression in human aortic endothelial cells (HAECs) and whether miRNA-200a/200b downregulate OGT expression to control HG-induced endothelial inflammation.
29720943	7	76	theme	THP-1	1429:1433	arg1	adhesion					1435:1442	THP-1 adhesion	1429:1442	THP-1 adhesion	1429:1442	Results: HG upregulated OGT mRNA and protein expression and protein O-GlcNAcylation levels (RL2 antibody) in HAECs, and showed increased intercellular adhesion molecule 1 (ICAM-1), vascular cell adhesion molecule 1 (VCAM-1), and E-selectin gene expression; ICAM-1 expression; and THP-1 adhesion.
29720943	12	77	theme	siRNA-mediated	2209:2222	arg1	depletion					2228:2236	siRNA-mediated OGT depletion	2209:2236	siRNA-mediated OGT depletion	2209:2236	Additionally, siRNA-mediated OGT depletion reduced HG-induced protein O-GlcNAcylation; ICAM-1, VCAM-1, and E-selectin gene expression; ICAM-1 expression; and THP-1 adhesion, confirming that HG-induced endothelial inflammation is partially mediated via OGT-induced protein O-GlcNAcylation.
29720943	5	78	theme	western	864:870	arg1	blotting					872:879	western blotting	864:879	western blotting	864:879	Methods: HAECs were stimulated with high glucose (25 mM) for 12 and 24 h. Real-time polymerase chain reaction (PCR), western blotting, THP-1 adhesion assay, bioinformatics predication, transfection of miR-200a/200b mimic or inhibitor, luciferase reporter assay, and transfection of siRNA OGT were performed.
29720943	5	79	dep	h.	818:819	arg1	performed					1044:1052	performed	1044:1052	were performed	1039:1052	Methods: HAECs were stimulated with high glucose (25 mM) for 12 and 24 h. Real-time polymerase chain reaction (PCR), western blotting, THP-1 adhesion assay, bioinformatics predication, transfection of miR-200a/200b mimic or inhibitor, luciferase reporter assay, and transfection of siRNA OGT were performed.
29720943	8	80	theme	homologous	1478:1487	arg1	sequences					1489:1497	homologous sequences	1478:1497	homologous sequences between members of the miR-200 family	1478:1535	Bioinformatics analysis revealed homologous sequences between members of the miR-200 family and the 3'-untranslated region (3'-UTR) of OGT mRNA, and real-time PCR analysis confirmed that members of miR-200 family were significantly decreased in HG-stimulated HAECs.
29720943	13	81	theme	db/db	2621:2625	arg1	mice					2627:2630	db/db mice	2621:2630	db/db mice	2621:2630	These results were validated in vivo: tail-vein injection of miR-200a/200b mimics downregulated endothelial OGT and ICAM-1 expression in db/db mice.
29720943	10	82	theme	reporter	1871:1878	arg1	assay					1880:1884	A luciferase reporter assay	1858:1884	A luciferase reporter assay	1858:1884	A luciferase reporter assay demonstrated that miR-200a/200b mimics bind to the 3'-UTR of OGT mRNA.
29720943	5	83	theme	bioinformatics	904:917	arg1	predication					919:929	bioinformatics predication	904:929	bioinformatics predication	904:929	Methods: HAECs were stimulated with high glucose (25 mM) for 12 and 24 h. Real-time polymerase chain reaction (PCR), western blotting, THP-1 adhesion assay, bioinformatics predication, transfection of miR-200a/200b mimic or inhibitor, luciferase reporter assay, and transfection of siRNA OGT were performed.
29720943	14	84	theme	therapeutic	2789:2799	arg1	role					2801:2804	the therapeutic role	2785:2804	the therapeutic role of miR-200a/200b on vascular complications in diabetes	2785:2859	Conclusion: miR-200a/200b are involved in modulating HG-induced endothelial inflammation by regulating OGT-mediated protein O-GlcNAcylation, suggesting the therapeutic role of miR-200a/200b on vascular complications in diabetes.
29720943	9	85	theme	impaired	1744:1751	arg1	restraint					1762:1770	an impaired feedback restraint	1741:1770	an impaired feedback restraint	1741:1770	This suggests the presence of an impaired feedback restraint on HG-induced endothelial protein O-GlcNAcylation levels because of OGT upregulation.
29720943	12	86	theme	OGT-induced	2447:2457	arg1	O-GlcNAcylation					2467:2481	OGT-induced protein O-GlcNAcylation	2447:2481	OGT-induced protein O-GlcNAcylation	2447:2481	Additionally, siRNA-mediated OGT depletion reduced HG-induced protein O-GlcNAcylation; ICAM-1, VCAM-1, and E-selectin gene expression; ICAM-1 expression; and THP-1 adhesion, confirming that HG-induced endothelial inflammation is partially mediated via OGT-induced protein O-GlcNAcylation.
29720943	7	87	theme	ICAM-1	1406:1411	arg1	expression					1413:1422	ICAM-1 expression	1406:1422	ICAM-1 expression	1406:1422	Results: HG upregulated OGT mRNA and protein expression and protein O-GlcNAcylation levels (RL2 antibody) in HAECs, and showed increased intercellular adhesion molecule 1 (ICAM-1), vascular cell adhesion molecule 1 (VCAM-1), and E-selectin gene expression; ICAM-1 expression; and THP-1 adhesion.
29720943	13	88	theme	endothelial	2580:2590	arg1	expression					2607:2616	endothelial OGT and ICAM-1 expression	2580:2616	endothelial OGT and ICAM-1 expression in db/db mice	2580:2630	These results were validated in vivo: tail-vein injection of miR-200a/200b mimics downregulated endothelial OGT and ICAM-1 expression in db/db mice.
29720943	11	89	theme	OGT	2031:2033	arg1	expression					2040:2049	HG-induced OGT mRNA expression	2020:2049	HG-induced OGT mRNA expression	2020:2049	Transfection with miR-200a/200b mimics significantly inhibited HG-induced OGT mRNA expression, OGT protein expression; protein O-GlcNAcylation levels; ICAM-1, VCAM-1, and E-selectin gene expression; ICAM-1 expression; and THP-1 adhesion.
29720943	5	90	theme	miR-200a/200b	948:960	arg1	reaction					848:855	Real-time polymerase chain reaction	821:855	Real-time polymerase chain reaction (PCR)	821:861	Methods: HAECs were stimulated with high glucose (25 mM) for 12 and 24 h. Real-time polymerase chain reaction (PCR), western blotting, THP-1 adhesion assay, bioinformatics predication, transfection of miR-200a/200b mimic or inhibitor, luciferase reporter assay, and transfection of siRNA OGT were performed.
29720943	5	90	theme	miR-200a/200b	948:960	arg1	blotting					872:879	western blotting	864:879	western blotting	864:879	Methods: HAECs were stimulated with high glucose (25 mM) for 12 and 24 h. Real-time polymerase chain reaction (PCR), western blotting, THP-1 adhesion assay, bioinformatics predication, transfection of miR-200a/200b mimic or inhibitor, luciferase reporter assay, and transfection of siRNA OGT were performed.
29720943	5	90	theme	miR-200a/200b	948:960	arg1	predication					919:929	bioinformatics predication	904:929	bioinformatics predication	904:929	Methods: HAECs were stimulated with high glucose (25 mM) for 12 and 24 h. Real-time polymerase chain reaction (PCR), western blotting, THP-1 adhesion assay, bioinformatics predication, transfection of miR-200a/200b mimic or inhibitor, luciferase reporter assay, and transfection of siRNA OGT were performed.
29720943	5	90	theme	miR-200a/200b	948:960	arg1	transfection					1013:1024	transfection	1013:1024	transfection of siRNA OGT	1013:1037	Methods: HAECs were stimulated with high glucose (25 mM) for 12 and 24 h. Real-time polymerase chain reaction (PCR), western blotting, THP-1 adhesion assay, bioinformatics predication, transfection of miR-200a/200b mimic or inhibitor, luciferase reporter assay, and transfection of siRNA OGT were performed.
29720943	5	90	theme	miR-200a/200b	948:960	arg1	transfection					932:943	transfection	932:943	transfection of miR-200a/200b mimic or inhibitor	932:979	Methods: HAECs were stimulated with high glucose (25 mM) for 12 and 24 h. Real-time polymerase chain reaction (PCR), western blotting, THP-1 adhesion assay, bioinformatics predication, transfection of miR-200a/200b mimic or inhibitor, luciferase reporter assay, and transfection of siRNA OGT were performed.
29720943	5	90	theme	miR-200a/200b	948:960	arg1	PCR					858:860	PCR	858:860	PCR	858:860	Methods: HAECs were stimulated with high glucose (25 mM) for 12 and 24 h. Real-time polymerase chain reaction (PCR), western blotting, THP-1 adhesion assay, bioinformatics predication, transfection of miR-200a/200b mimic or inhibitor, luciferase reporter assay, and transfection of siRNA OGT were performed.
29720943	5	90	theme	miR-200a/200b	948:960	arg1	assay					897:901	THP-1 adhesion assay	882:901	THP-1 adhesion assay	882:901	Methods: HAECs were stimulated with high glucose (25 mM) for 12 and 24 h. Real-time polymerase chain reaction (PCR), western blotting, THP-1 adhesion assay, bioinformatics predication, transfection of miR-200a/200b mimic or inhibitor, luciferase reporter assay, and transfection of siRNA OGT were performed.
29720943	5	90	theme	miR-200a/200b	948:960	arg1	assay					1002:1006	luciferase reporter assay	982:1006	luciferase reporter assay	982:1006	Methods: HAECs were stimulated with high glucose (25 mM) for 12 and 24 h. Real-time polymerase chain reaction (PCR), western blotting, THP-1 adhesion assay, bioinformatics predication, transfection of miR-200a/200b mimic or inhibitor, luciferase reporter assay, and transfection of siRNA OGT were performed.
29720943	9	91	theme	restraint	1762:1770	arg1	presence					1729:1736	the presence	1725:1736	the presence of an impaired feedback restraint on HG-induced endothelial protein O-GlcNAcylation levels	1725:1827	This suggests the presence of an impaired feedback restraint on HG-induced endothelial protein O-GlcNAcylation levels because of OGT upregulation.
29720943	12	92	theme	endothelial	2396:2406	arg1	inflammation					2408:2419	HG-induced endothelial inflammation	2385:2419	HG-induced endothelial inflammation	2385:2419	Additionally, siRNA-mediated OGT depletion reduced HG-induced protein O-GlcNAcylation; ICAM-1, VCAM-1, and E-selectin gene expression; ICAM-1 expression; and THP-1 adhesion, confirming that HG-induced endothelial inflammation is partially mediated via OGT-induced protein O-GlcNAcylation.
29720943	11	93	dep	mimics	1989:1994	arg1	inhibited					2010:2018	inhibited	2010:2018	mimics significantly inhibited HG-induced OGT mRNA expression, OGT protein expression; protein O-GlcNAcylation levels; ICAM-1, VCAM-1, and E-selectin gene expression; ICAM-1 expression; and THP-1 adhesion	1989:2192	Transfection with miR-200a/200b mimics significantly inhibited HG-induced OGT mRNA expression, OGT protein expression; protein O-GlcNAcylation levels; ICAM-1, VCAM-1, and E-selectin gene expression; ICAM-1 expression; and THP-1 adhesion.
29720943	1	94	theme	O-linked	171:178	arg1	O-GlcNAc					201:208	O-GlcNAc	201:208	O-GlcNAc	201:208	Background and Aims: Increased O-linked N-acetylglucosamine (O-GlcNAc) modification of proteins by O-GlcNAc transferase (OGT) is associated with diabetic complications.
29720943	1	94	theme	O-linked	171:178	arg1	N-acetylglucosamine					180:198	O-linked N-acetylglucosamine	171:198	Increased O-linked N-acetylglucosamine (O-GlcNAc) modification of proteins by O-GlcNAc transferase (OGT)	161:264	Background and Aims: Increased O-linked N-acetylglucosamine (O-GlcNAc) modification of proteins by O-GlcNAc transferase (OGT) is associated with diabetic complications.
29720943	9	95	theme	HG-induced	1775:1784	arg1	levels					1822:1827	HG-induced endothelial protein O-GlcNAcylation levels	1775:1827	HG-induced endothelial protein O-GlcNAcylation levels	1775:1827	This suggests the presence of an impaired feedback restraint on HG-induced endothelial protein O-GlcNAcylation levels because of OGT upregulation.
29720943	8	96	theme	miR-200	1522:1528	arg1	family					1530:1535	the miR-200 family	1518:1535	the miR-200 family	1518:1535	Bioinformatics analysis revealed homologous sequences between members of the miR-200 family and the 3'-untranslated region (3'-UTR) of OGT mRNA, and real-time PCR analysis confirmed that members of miR-200 family were significantly decreased in HG-stimulated HAECs.
29720943	7	97	theme	E-selectin	1378:1387	arg1	expression					1394:1403	E-selectin gene expression	1378:1403	E-selectin gene expression	1378:1403	Results: HG upregulated OGT mRNA and protein expression and protein O-GlcNAcylation levels (RL2 antibody) in HAECs, and showed increased intercellular adhesion molecule 1 (ICAM-1), vascular cell adhesion molecule 1 (VCAM-1), and E-selectin gene expression; ICAM-1 expression; and THP-1 adhesion.
29720943	0	98	theme	N-Acetylglucosamine	96:114	arg1	Transferase					116:126	O-linked N-Acetylglucosamine Transferase	87:126	O-linked N-Acetylglucosamine Transferase Expression	87:137	MicroRNA-200a/200b Modulate High Glucose-Induced Endothelial Inflammation by Targeting O-linked N-Acetylglucosamine Transferase Expression.
29720943	11	99	theme	OGT	2052:2054	arg1	expression					2064:2073	OGT protein expression	2052:2073	OGT protein expression	2052:2073	Transfection with miR-200a/200b mimics significantly inhibited HG-induced OGT mRNA expression, OGT protein expression; protein O-GlcNAcylation levels; ICAM-1, VCAM-1, and E-selectin gene expression; ICAM-1 expression; and THP-1 adhesion.
29720943	14	100	theme	OGT-mediated	2736:2747	arg1	O-GlcNAcylation					2757:2771	OGT-mediated protein O-GlcNAcylation	2736:2771	OGT-mediated protein O-GlcNAcylation	2736:2771	Conclusion: miR-200a/200b are involved in modulating HG-induced endothelial inflammation by regulating OGT-mediated protein O-GlcNAcylation, suggesting the therapeutic role of miR-200a/200b on vascular complications in diabetes.
29720943	9	101	theme	protein	1798:1804	arg1	levels					1822:1827	HG-induced endothelial protein O-GlcNAcylation levels	1775:1827	HG-induced endothelial protein O-GlcNAcylation levels	1775:1827	This suggests the presence of an impaired feedback restraint on HG-induced endothelial protein O-GlcNAcylation levels because of OGT upregulation.
29720943	14	102	from	complications	2835:2847	arg1	diabetes					2852:2859	diabetes	2852:2859	diabetes	2852:2859	Conclusion: miR-200a/200b are involved in modulating HG-induced endothelial inflammation by regulating OGT-mediated protein O-GlcNAcylation, suggesting the therapeutic role of miR-200a/200b on vascular complications in diabetes.
29720943	3	103	theme	previous	392:399	arg1	study					401:405	A previous study	390:405	A previous study	390:405	A previous study reported that microRNA-200 (miR-200) family members are sensitive to oxidative stress.
29720943	7	104	from	expression	1194:1203	arg1	HAECs					1258:1262	HAECs	1258:1262	HAECs	1258:1262	Results: HG upregulated OGT mRNA and protein expression and protein O-GlcNAcylation levels (RL2 antibody) in HAECs, and showed increased intercellular adhesion molecule 1 (ICAM-1), vascular cell adhesion molecule 1 (VCAM-1), and E-selectin gene expression; ICAM-1 expression; and THP-1 adhesion.
29720943	1	105	dep	Background	140:149	arg1	associated					269:278	associated	269:278	is associated with diabetic complications	266:306	Background and Aims: Increased O-linked N-acetylglucosamine (O-GlcNAc) modification of proteins by O-GlcNAc transferase (OGT) is associated with diabetic complications.
29720943	9	106	from	presence	1729:1736	arg1	levels					1822:1827	HG-induced endothelial protein O-GlcNAcylation levels	1775:1827	HG-induced endothelial protein O-GlcNAcylation levels	1775:1827	This suggests the presence of an impaired feedback restraint on HG-induced endothelial protein O-GlcNAcylation levels because of OGT upregulation.
29720943	12	107	theme	gene	2313:2316	arg1	expression					2318:2327	ICAM-1, VCAM-1, and E-selectin gene expression	2282:2327	ICAM-1, VCAM-1, and E-selectin gene expression	2282:2327	Additionally, siRNA-mediated OGT depletion reduced HG-induced protein O-GlcNAcylation; ICAM-1, VCAM-1, and E-selectin gene expression; ICAM-1 expression; and THP-1 adhesion, confirming that HG-induced endothelial inflammation is partially mediated via OGT-induced protein O-GlcNAcylation.
29720943	7	108	theme	RL2	1241:1243	arg1	levels					1233:1238	protein O-GlcNAcylation levels	1209:1238	protein O-GlcNAcylation levels (RL2 antibody)	1209:1253	Results: HG upregulated OGT mRNA and protein expression and protein O-GlcNAcylation levels (RL2 antibody) in HAECs, and showed increased intercellular adhesion molecule 1 (ICAM-1), vascular cell adhesion molecule 1 (VCAM-1), and E-selectin gene expression; ICAM-1 expression; and THP-1 adhesion.
29720943	7	108	theme	RL2	1241:1243	arg1	antibody					1245:1252	RL2 antibody	1241:1252	RL2 antibody	1241:1252	Results: HG upregulated OGT mRNA and protein expression and protein O-GlcNAcylation levels (RL2 antibody) in HAECs, and showed increased intercellular adhesion molecule 1 (ICAM-1), vascular cell adhesion molecule 1 (VCAM-1), and E-selectin gene expression; ICAM-1 expression; and THP-1 adhesion.
29720943	13	109	theme	mimics	2559:2564	arg1	injection					2532:2540	tail-vein injection	2522:2540	tail-vein injection of miR-200a/200b mimics	2522:2564	These results were validated in vivo: tail-vein injection of miR-200a/200b mimics downregulated endothelial OGT and ICAM-1 expression in db/db mice.
29720943	6	110	theme	immunohistochemistry	1118:1137	arg1	staining					1139:1146	immunohistochemistry staining	1118:1146	immunohistochemistry staining	1118:1146	The aortic endothelium of db/db diabetic mice was evaluated by immunohistochemistry staining.
29720943	14	111	theme	HG-induced	2686:2695	arg1	inflammation					2709:2720	HG-induced endothelial inflammation	2686:2720	HG-induced endothelial inflammation	2686:2720	Conclusion: miR-200a/200b are involved in modulating HG-induced endothelial inflammation by regulating OGT-mediated protein O-GlcNAcylation, suggesting the therapeutic role of miR-200a/200b on vascular complications in diabetes.
29720943	7	112	from	levels	1233:1238	arg1	HAECs					1258:1262	HAECs	1258:1262	HAECs	1258:1262	Results: HG upregulated OGT mRNA and protein expression and protein O-GlcNAcylation levels (RL2 antibody) in HAECs, and showed increased intercellular adhesion molecule 1 (ICAM-1), vascular cell adhesion molecule 1 (VCAM-1), and E-selectin gene expression; ICAM-1 expression; and THP-1 adhesion.
29720943	8	113	theme	OGT	1580:1582	arg1	mRNA					1584:1587	OGT mRNA	1580:1587	OGT mRNA	1580:1587	Bioinformatics analysis revealed homologous sequences between members of the miR-200 family and the 3'-untranslated region (3'-UTR) of OGT mRNA, and real-time PCR analysis confirmed that members of miR-200 family were significantly decreased in HG-stimulated HAECs.
29720943	12	114	theme	VCAM-1	2290:2295	arg1	expression					2318:2327	ICAM-1, VCAM-1, and E-selectin gene expression	2282:2327	ICAM-1, VCAM-1, and E-selectin gene expression	2282:2327	Additionally, siRNA-mediated OGT depletion reduced HG-induced protein O-GlcNAcylation; ICAM-1, VCAM-1, and E-selectin gene expression; ICAM-1 expression; and THP-1 adhesion, confirming that HG-induced endothelial inflammation is partially mediated via OGT-induced protein O-GlcNAcylation.
29720943	0	115	theme	Glucose-Induced	33:47	arg1	Inflammation					61:72	High Glucose-Induced Endothelial Inflammation	28:72	High Glucose-Induced Endothelial Inflammation	28:72	MicroRNA-200a/200b Modulate High Glucose-Induced Endothelial Inflammation by Targeting O-linked N-Acetylglucosamine Transferase Expression.
29720943	13	116	from	expression	2607:2616	arg1	mice					2627:2630	db/db mice	2621:2630	db/db mice	2621:2630	These results were validated in vivo: tail-vein injection of miR-200a/200b mimics downregulated endothelial OGT and ICAM-1 expression in db/db mice.
29720943	5	117	theme	reporter	993:1000	arg1	assay					1002:1006	luciferase reporter assay	982:1006	luciferase reporter assay	982:1006	Methods: HAECs were stimulated with high glucose (25 mM) for 12 and 24 h. Real-time polymerase chain reaction (PCR), western blotting, THP-1 adhesion assay, bioinformatics predication, transfection of miR-200a/200b mimic or inhibitor, luciferase reporter assay, and transfection of siRNA OGT were performed.
29720943	6	118	theme	mice	1096:1099	arg1	endothelium					1066:1076	The aortic endothelium	1055:1076	The aortic endothelium of db/db diabetic mice	1055:1099	The aortic endothelium of db/db diabetic mice was evaluated by immunohistochemistry staining.
29720943	3	119	theme	family	444:449	arg1	members					451:457	microRNA-200 (miR-200) family members	421:457	microRNA-200 (miR-200) family members	421:457	A previous study reported that microRNA-200 (miR-200) family members are sensitive to oxidative stress.
29720943	12	120	theme	ICAM-1	2330:2335	arg1	expression					2337:2346	ICAM-1 expression	2330:2346	ICAM-1 expression	2330:2346	Additionally, siRNA-mediated OGT depletion reduced HG-induced protein O-GlcNAcylation; ICAM-1, VCAM-1, and E-selectin gene expression; ICAM-1 expression; and THP-1 adhesion, confirming that HG-induced endothelial inflammation is partially mediated via OGT-induced protein O-GlcNAcylation.
29720943	11	121	theme	HG-induced	2020:2029	arg1	expression					2040:2049	HG-induced OGT mRNA expression	2020:2049	HG-induced OGT mRNA expression	2020:2049	Transfection with miR-200a/200b mimics significantly inhibited HG-induced OGT mRNA expression, OGT protein expression; protein O-GlcNAcylation levels; ICAM-1, VCAM-1, and E-selectin gene expression; ICAM-1 expression; and THP-1 adhesion.
29720943	5	122	theme	Real-time	821:829	arg1	PCR					858:860	PCR	858:860	PCR	858:860	Methods: HAECs were stimulated with high glucose (25 mM) for 12 and 24 h. Real-time polymerase chain reaction (PCR), western blotting, THP-1 adhesion assay, bioinformatics predication, transfection of miR-200a/200b mimic or inhibitor, luciferase reporter assay, and transfection of siRNA OGT were performed.
29720943	5	122	theme	Real-time	821:829	arg1	reaction					848:855	Real-time polymerase chain reaction	821:855	Real-time polymerase chain reaction (PCR)	821:861	Methods: HAECs were stimulated with high glucose (25 mM) for 12 and 24 h. Real-time polymerase chain reaction (PCR), western blotting, THP-1 adhesion assay, bioinformatics predication, transfection of miR-200a/200b mimic or inhibitor, luciferase reporter assay, and transfection of siRNA OGT were performed.
29720943	6	123	theme	db/db	1081:1085	arg1	mice					1096:1099	db/db diabetic mice	1081:1099	db/db diabetic mice	1081:1099	The aortic endothelium of db/db diabetic mice was evaluated by immunohistochemistry staining.
29720943	8	124	theme	real-time	1594:1602	arg1	analysis					1608:1615	real-time PCR analysis	1594:1615	real-time PCR analysis	1594:1615	Bioinformatics analysis revealed homologous sequences between members of the miR-200 family and the 3'-untranslated region (3'-UTR) of OGT mRNA, and real-time PCR analysis confirmed that members of miR-200 family were significantly decreased in HG-stimulated HAECs.
29720943	5	125	theme	chain	842:846	arg1	PCR					858:860	PCR	858:860	PCR	858:860	Methods: HAECs were stimulated with high glucose (25 mM) for 12 and 24 h. Real-time polymerase chain reaction (PCR), western blotting, THP-1 adhesion assay, bioinformatics predication, transfection of miR-200a/200b mimic or inhibitor, luciferase reporter assay, and transfection of siRNA OGT were performed.
29720943	5	125	theme	chain	842:846	arg1	reaction					848:855	Real-time polymerase chain reaction	821:855	Real-time polymerase chain reaction (PCR)	821:861	Methods: HAECs were stimulated with high glucose (25 mM) for 12 and 24 h. Real-time polymerase chain reaction (PCR), western blotting, THP-1 adhesion assay, bioinformatics predication, transfection of miR-200a/200b mimic or inhibitor, luciferase reporter assay, and transfection of siRNA OGT were performed.
29720943	12	126	theme	THP-1	2353:2357	arg1	adhesion					2359:2366	THP-1 adhesion	2353:2366	THP-1 adhesion	2353:2366	Additionally, siRNA-mediated OGT depletion reduced HG-induced protein O-GlcNAcylation; ICAM-1, VCAM-1, and E-selectin gene expression; ICAM-1 expression; and THP-1 adhesion, confirming that HG-induced endothelial inflammation is partially mediated via OGT-induced protein O-GlcNAcylation.
29720943	11	127	theme	E-selectin	2128:2137	arg1	expression					2144:2153	E-selectin gene expression	2128:2153	E-selectin gene expression	2128:2153	Transfection with miR-200a/200b mimics significantly inhibited HG-induced OGT mRNA expression, OGT protein expression; protein O-GlcNAcylation levels; ICAM-1, VCAM-1, and E-selectin gene expression; ICAM-1 expression; and THP-1 adhesion.
29720943	8	128	theme	mRNA	1584:1587	arg1	sequences					1489:1497	homologous sequences	1478:1497	homologous sequences between members of the miR-200 family	1478:1535	Bioinformatics analysis revealed homologous sequences between members of the miR-200 family and the 3'-untranslated region (3'-UTR) of OGT mRNA, and real-time PCR analysis confirmed that members of miR-200 family were significantly decreased in HG-stimulated HAECs.
29720943	8	128	theme	mRNA	1584:1587	arg1	3'-UTR					1569:1574	3'-UTR	1569:1574	3'-UTR	1569:1574	Bioinformatics analysis revealed homologous sequences between members of the miR-200 family and the 3'-untranslated region (3'-UTR) of OGT mRNA, and real-time PCR analysis confirmed that members of miR-200 family were significantly decreased in HG-stimulated HAECs.
29720943	8	128	theme	mRNA	1584:1587	arg1	region					1561:1566	the 3'-untranslated region	1541:1566	the 3'-untranslated region (3'-UTR) of OGT mRNA	1541:1587	Bioinformatics analysis revealed homologous sequences between members of the miR-200 family and the 3'-untranslated region (3'-UTR) of OGT mRNA, and real-time PCR analysis confirmed that members of miR-200 family were significantly decreased in HG-stimulated HAECs.
29720943	12	129	theme	ICAM-1	2282:2287	arg1	expression					2318:2327	ICAM-1, VCAM-1, and E-selectin gene expression	2282:2327	ICAM-1, VCAM-1, and E-selectin gene expression	2282:2327	Additionally, siRNA-mediated OGT depletion reduced HG-induced protein O-GlcNAcylation; ICAM-1, VCAM-1, and E-selectin gene expression; ICAM-1 expression; and THP-1 adhesion, confirming that HG-induced endothelial inflammation is partially mediated via OGT-induced protein O-GlcNAcylation.
29720943	9	130	attach	presence	1729:1736	arg2	restraint					1762:1770	an impaired feedback restraint	1741:1770	an impaired feedback restraint	1741:1770	This suggests the presence of an impaired feedback restraint on HG-induced endothelial protein O-GlcNAcylation levels because of OGT upregulation.
29720943	9	130	attach	presence	1729:1736	arg1	levels					1822:1827	HG-induced endothelial protein O-GlcNAcylation levels	1775:1827	HG-induced endothelial protein O-GlcNAcylation levels	1775:1827	This suggests the presence of an impaired feedback restraint on HG-induced endothelial protein O-GlcNAcylation levels because of OGT upregulation.
29720943	12	131	theme	HG-induced	2246:2255	arg1	O-GlcNAcylation					2265:2279	HG-induced protein O-GlcNAcylation	2246:2279	HG-induced protein O-GlcNAcylation	2246:2279	Additionally, siRNA-mediated OGT depletion reduced HG-induced protein O-GlcNAcylation; ICAM-1, VCAM-1, and E-selectin gene expression; ICAM-1 expression; and THP-1 adhesion, confirming that HG-induced endothelial inflammation is partially mediated via OGT-induced protein O-GlcNAcylation.
29720943	5	132	theme	siRNA	1029:1033	arg1	OGT					1035:1037	siRNA OGT	1029:1037	siRNA OGT	1029:1037	Methods: HAECs were stimulated with high glucose (25 mM) for 12 and 24 h. Real-time polymerase chain reaction (PCR), western blotting, THP-1 adhesion assay, bioinformatics predication, transfection of miR-200a/200b mimic or inhibitor, luciferase reporter assay, and transfection of siRNA OGT were performed.
29720943	0	133	link	O-linked	87:94	arg1	Transferase					116:126	O-linked N-Acetylglucosamine Transferase	87:126	O-linked N-Acetylglucosamine Transferase Expression	87:137	MicroRNA-200a/200b Modulate High Glucose-Induced Endothelial Inflammation by Targeting O-linked N-Acetylglucosamine Transferase Expression.
29720943	7	134	theme	adhesion	1300:1307	arg1	molecule					1309:1316	increased intercellular adhesion molecule 1	1276:1318	increased intercellular adhesion molecule 1 (ICAM-1)	1276:1327	Results: HG upregulated OGT mRNA and protein expression and protein O-GlcNAcylation levels (RL2 antibody) in HAECs, and showed increased intercellular adhesion molecule 1 (ICAM-1), vascular cell adhesion molecule 1 (VCAM-1), and E-selectin gene expression; ICAM-1 expression; and THP-1 adhesion.
29720943	7	134	theme	adhesion	1300:1307	arg1	ICAM-1					1321:1326	ICAM-1	1321:1326	ICAM-1	1321:1326	Results: HG upregulated OGT mRNA and protein expression and protein O-GlcNAcylation levels (RL2 antibody) in HAECs, and showed increased intercellular adhesion molecule 1 (ICAM-1), vascular cell adhesion molecule 1 (VCAM-1), and E-selectin gene expression; ICAM-1 expression; and THP-1 adhesion.
29720943	4	135	theme	OGT	684:686	arg1	expression					688:697	OGT expression	684:697	OGT expression	684:697	In this study, we examined whether miR-200a and miR-200b regulate high-glucose (HG)-induced OGT expression in human aortic endothelial cells (HAECs) and whether miRNA-200a/200b downregulate OGT expression to control HG-induced endothelial inflammation.
29720943	8	136	theme	Bioinformatics	1445:1458	arg1	analysis					1460:1467	Bioinformatics analysis	1445:1467	Bioinformatics analysis	1445:1467	Bioinformatics analysis revealed homologous sequences between members of the miR-200 family and the 3'-untranslated region (3'-UTR) of OGT mRNA, and real-time PCR analysis confirmed that members of miR-200 family were significantly decreased in HG-stimulated HAECs.
29720943	1	137	theme	diabetic	285:292	arg1	complications					294:306	diabetic complications	285:306	diabetic complications	285:306	Background and Aims: Increased O-linked N-acetylglucosamine (O-GlcNAc) modification of proteins by O-GlcNAc transferase (OGT) is associated with diabetic complications.
29713168	5	0	theme	protein-loading	953:967	arg1	efficiency					969:978	High protein-loading efficiency	948:978	High protein-loading efficiency	948:978	High protein-loading efficiency was obtained by testing with the model antigen ovalbumin (Ova), and the Ova adsorbed onto the cationic CS/O-HTCC complexes was taken up easily by the epithelium.
29713168	6	1	theme	vaccinated	1323:1332	arg1	mice					1334:1337	intranasally vaccinated mice	1310:1337	intranasally vaccinated mice	1310:1337	To evaluate the capacity of the Ova/CS/O-HTCC nanoparticles for immune enhancement in vivo, we collected and analyzed immunocytes, serum, and mucosal lavage fluid from intranasally vaccinated mice.
29713168	1	2	theme	ideal	193:197	arg1	adjuvants					207:215	ideal vaccine adjuvants	193:215	ideal vaccine adjuvants for intranasal vaccination	193:242	INTRODUCTION The development of ideal vaccine adjuvants for intranasal vaccination can provide convenience for many vaccinations.
29713168	0	3	theme	exogenous	114:122	arg1	antigens					124:131	exogenous antigens	114:131	exogenous antigens	114:131	Curdlan sulfate-O-linked quaternized chitosan nanoparticles: potential adjuvants to improve the immunogenicity of exogenous antigens via intranasal vaccination.
29713168	3	4	theme	propyl-3-trimethyl	636:653	arg1	chloride					673:680	curdlan sulfate (CS)-O-(2-hydroxyl)propyl-3-trimethyl ammonium chitosan chloride	601:680	curdlan sulfate (CS)-O-(2-hydroxyl)propyl-3-trimethyl ammonium chitosan chloride (O-HTCC) nanoparticles	601:703	METHODS By using the advantages of polysaccharides, which can promote both T-helper 1 and 2 responses, curdlan sulfate (CS)-O-(2-hydroxyl)propyl-3-trimethyl ammonium chitosan chloride (O-HTCC) nanoparticles were prepared by interacting CS with O-HTCC, and the adjuvancy of the nanoparticles was investigated.
29713168	3	4	theme	propyl-3-trimethyl	636:653	arg1	O-HTCC					683:688	O-HTCC	683:688	O-HTCC	683:688	METHODS By using the advantages of polysaccharides, which can promote both T-helper 1 and 2 responses, curdlan sulfate (CS)-O-(2-hydroxyl)propyl-3-trimethyl ammonium chitosan chloride (O-HTCC) nanoparticles were prepared by interacting CS with O-HTCC, and the adjuvancy of the nanoparticles was investigated.
29713168	4	5	theme	cells	941:945	arg1	proliferation					890:902	proliferation	890:902	proliferation	890:902	RESULTS The results showed that the polysaccharide-based nanoparticles induced the proliferation and activation of antigen-presenting cells.
29713168	4	5	theme	cells	941:945	arg1	activation					908:917	activation	908:917	activation	908:917	RESULTS The results showed that the polysaccharide-based nanoparticles induced the proliferation and activation of antigen-presenting cells.
29713168	6	6	theme	Ova/CS/O-HTCC	1174:1186	arg1	nanoparticles					1188:1200	the Ova/CS/O-HTCC nanoparticles	1170:1200	the Ova/CS/O-HTCC nanoparticles	1170:1200	To evaluate the capacity of the Ova/CS/O-HTCC nanoparticles for immune enhancement in vivo, we collected and analyzed immunocytes, serum, and mucosal lavage fluid from intranasally vaccinated mice.
29713168	6	7	theme	immune	1206:1211	arg1	enhancement					1213:1223	immune enhancement	1206:1223	immune enhancement	1206:1223	To evaluate the capacity of the Ova/CS/O-HTCC nanoparticles for immune enhancement in vivo, we collected and analyzed immunocytes, serum, and mucosal lavage fluid from intranasally vaccinated mice.
29713168	4	8	theme	antigen-presenting	922:939	arg1	cells					941:945	antigen-presenting cells	922:945	antigen-presenting cells	922:945	RESULTS The results showed that the polysaccharide-based nanoparticles induced the proliferation and activation of antigen-presenting cells.
29713168	0	9	link	sulfate-O-linked	8:23	arg1	nanoparticles					46:58	Curdlan sulfate-O-linked quaternized chitosan nanoparticles	0:58	Curdlan sulfate-O-linked quaternized chitosan nanoparticles: potential adjuvants to improve the immunogenicity of exogenous antigens via intranasal vaccination.	0:159	Curdlan sulfate-O-linked quaternized chitosan nanoparticles: potential adjuvants to improve the immunogenicity of exogenous antigens via intranasal vaccination.
29713168	1	10	dep	INTRODUCTION	161:172	arg1	development					178:188	The development	174:188	INTRODUCTION The development of ideal vaccine adjuvants for intranasal vaccination	161:242	INTRODUCTION The development of ideal vaccine adjuvants for intranasal vaccination can provide convenience for many vaccinations.
29713168	2	11	theme	adjuvant	322:329	arg1	vaccine					314:320	an ideal intranasal vaccine adjuvant	294:329	an ideal intranasal vaccine adjuvant	294:329	As an ideal intranasal vaccine adjuvant, it should have the properties of assisting soluble antigens to pass the mucosal barrier and potentiating both systemic and mucosal immunity via nasal administration.
29713168	2	11	theme	adjuvant	322:329	arg1	properties					351:360	the properties	347:360	the properties of assisting soluble antigens to pass the mucosal barrier and potentiating both systemic and mucosal immunity via nasal administration	347:495	As an ideal intranasal vaccine adjuvant, it should have the properties of assisting soluble antigens to pass the mucosal barrier and potentiating both systemic and mucosal immunity via nasal administration.
29713168	1	12	theme	vaccine	199:205	arg1	adjuvants					207:215	ideal vaccine adjuvants	193:215	ideal vaccine adjuvants for intranasal vaccination	193:242	INTRODUCTION The development of ideal vaccine adjuvants for intranasal vaccination can provide convenience for many vaccinations.
29713168	0	13	theme	antigens	124:131	arg1	immunogenicity					96:109	the immunogenicity	92:109	the immunogenicity of exogenous antigens	92:131	Curdlan sulfate-O-linked quaternized chitosan nanoparticles: potential adjuvants to improve the immunogenicity of exogenous antigens via intranasal vaccination.
29713168	6	14	theme	lavage	1292:1297	arg1	fluid					1299:1303	mucosal lavage fluid	1284:1303	mucosal lavage fluid	1284:1303	To evaluate the capacity of the Ova/CS/O-HTCC nanoparticles for immune enhancement in vivo, we collected and analyzed immunocytes, serum, and mucosal lavage fluid from intranasally vaccinated mice.
29713168	4	15	dep	proliferation	890:902	arg1	the					886:888	the	886:888	the	886:888	RESULTS The results showed that the polysaccharide-based nanoparticles induced the proliferation and activation of antigen-presenting cells.
29713168	1	16	theme	adjuvants	207:215	arg1	development					178:188	The development	174:188	INTRODUCTION The development of ideal vaccine adjuvants for intranasal vaccination	161:242	INTRODUCTION The development of ideal vaccine adjuvants for intranasal vaccination can provide convenience for many vaccinations.
29713168	0	17	theme	sulfate-O-linked	8:23	arg1	nanoparticles					46:58	Curdlan sulfate-O-linked quaternized chitosan nanoparticles	0:58	Curdlan sulfate-O-linked quaternized chitosan nanoparticles: potential adjuvants to improve the immunogenicity of exogenous antigens via intranasal vaccination.	0:159	Curdlan sulfate-O-linked quaternized chitosan nanoparticles: potential adjuvants to improve the immunogenicity of exogenous antigens via intranasal vaccination.
29713168	6	18	theme	mucosal	1284:1290	arg1	fluid					1299:1303	mucosal lavage fluid	1284:1303	mucosal lavage fluid	1284:1303	To evaluate the capacity of the Ova/CS/O-HTCC nanoparticles for immune enhancement in vivo, we collected and analyzed immunocytes, serum, and mucosal lavage fluid from intranasally vaccinated mice.
29713168	3	19	theme	curdlan	601:607	arg1	CS					618:619	CS	618:619	CS	618:619	METHODS By using the advantages of polysaccharides, which can promote both T-helper 1 and 2 responses, curdlan sulfate (CS)-O-(2-hydroxyl)propyl-3-trimethyl ammonium chitosan chloride (O-HTCC) nanoparticles were prepared by interacting CS with O-HTCC, and the adjuvancy of the nanoparticles was investigated.
29713168	3	19	theme	curdlan	601:607	arg1	sulfate					609:615	curdlan sulfate	601:615	curdlan sulfate (CS)-O-(2-hydroxyl)propyl-3-trimethyl ammonium chitosan chloride (O-HTCC) nanoparticles	601:703	METHODS By using the advantages of polysaccharides, which can promote both T-helper 1 and 2 responses, curdlan sulfate (CS)-O-(2-hydroxyl)propyl-3-trimethyl ammonium chitosan chloride (O-HTCC) nanoparticles were prepared by interacting CS with O-HTCC, and the adjuvancy of the nanoparticles was investigated.
29713168	9	20	theme	soluble	1802:1808	arg1	antigens					1810:1817	soluble antigens	1802:1817	soluble antigens used in intranasal or mucosal vaccination	1802:1859	CONCLUSION Therefore, these results suggest that CS/O-HTCC nanoparticles are ideal vaccine adjuvants for soluble antigens used in intranasal or mucosal vaccination.
29713168	9	21	dep	CONCLUSION	1697:1706	arg1	suggest					1733:1739	suggest	1733:1739	suggest that CS/O-HTCC nanoparticles are ideal vaccine adjuvants for soluble antigens used in intranasal or mucosal vaccination	1733:1859	CONCLUSION Therefore, these results suggest that CS/O-HTCC nanoparticles are ideal vaccine adjuvants for soluble antigens used in intranasal or mucosal vaccination.
29713168	0	22	theme	Curdlan	0:6	arg1	nanoparticles					46:58	Curdlan sulfate-O-linked quaternized chitosan nanoparticles	0:58	Curdlan sulfate-O-linked quaternized chitosan nanoparticles: potential adjuvants to improve the immunogenicity of exogenous antigens via intranasal vaccination.	0:159	Curdlan sulfate-O-linked quaternized chitosan nanoparticles: potential adjuvants to improve the immunogenicity of exogenous antigens via intranasal vaccination.
29713168	3	23	dep	prepared	710:717	arg1	promote					560:566	promote	560:566	can promote both T-helper 1 and 2 responses	556:598	METHODS By using the advantages of polysaccharides, which can promote both T-helper 1 and 2 responses, curdlan sulfate (CS)-O-(2-hydroxyl)propyl-3-trimethyl ammonium chitosan chloride (O-HTCC) nanoparticles were prepared by interacting CS with O-HTCC, and the adjuvancy of the nanoparticles was investigated.
29713168	3	23	dep	prepared	710:717	arg1	METHODS					498:504	METHODS	498:504	METHODS By using the advantages of polysaccharides	498:547	METHODS By using the advantages of polysaccharides, which can promote both T-helper 1 and 2 responses, curdlan sulfate (CS)-O-(2-hydroxyl)propyl-3-trimethyl ammonium chitosan chloride (O-HTCC) nanoparticles were prepared by interacting CS with O-HTCC, and the adjuvancy of the nanoparticles was investigated.
29713168	3	24	theme	sulfate	609:615	arg1	chloride					673:680	curdlan sulfate (CS)-O-(2-hydroxyl)propyl-3-trimethyl ammonium chitosan chloride	601:680	curdlan sulfate (CS)-O-(2-hydroxyl)propyl-3-trimethyl ammonium chitosan chloride (O-HTCC) nanoparticles	601:703	METHODS By using the advantages of polysaccharides, which can promote both T-helper 1 and 2 responses, curdlan sulfate (CS)-O-(2-hydroxyl)propyl-3-trimethyl ammonium chitosan chloride (O-HTCC) nanoparticles were prepared by interacting CS with O-HTCC, and the adjuvancy of the nanoparticles was investigated.
29713168	3	24	theme	sulfate	609:615	arg1	O-HTCC					683:688	O-HTCC	683:688	O-HTCC	683:688	METHODS By using the advantages of polysaccharides, which can promote both T-helper 1 and 2 responses, curdlan sulfate (CS)-O-(2-hydroxyl)propyl-3-trimethyl ammonium chitosan chloride (O-HTCC) nanoparticles were prepared by interacting CS with O-HTCC, and the adjuvancy of the nanoparticles was investigated.
29713168	1	25	theme	intranasal	221:230	arg1	vaccination					232:242	intranasal vaccination	221:242	intranasal vaccination	221:242	INTRODUCTION The development of ideal vaccine adjuvants for intranasal vaccination can provide convenience for many vaccinations.
29713168	0	26	theme	chitosan	37:44	arg1	nanoparticles					46:58	Curdlan sulfate-O-linked quaternized chitosan nanoparticles	0:58	Curdlan sulfate-O-linked quaternized chitosan nanoparticles: potential adjuvants to improve the immunogenicity of exogenous antigens via intranasal vaccination.	0:159	Curdlan sulfate-O-linked quaternized chitosan nanoparticles: potential adjuvants to improve the immunogenicity of exogenous antigens via intranasal vaccination.
29713168	5	27	theme	cationic	1074:1081	arg1	complexes					1093:1101	the cationic CS/O-HTCC complexes	1070:1101	the cationic CS/O-HTCC complexes	1070:1101	High protein-loading efficiency was obtained by testing with the model antigen ovalbumin (Ova), and the Ova adsorbed onto the cationic CS/O-HTCC complexes was taken up easily by the epithelium.
29713168	5	28	theme	model	1013:1017	arg1	Ova					1038:1040	Ova	1038:1040	Ova	1038:1040	High protein-loading efficiency was obtained by testing with the model antigen ovalbumin (Ova), and the Ova adsorbed onto the cationic CS/O-HTCC complexes was taken up easily by the epithelium.
29713168	5	28	theme	model	1013:1017	arg1	ovalbumin					1027:1035	the model antigen ovalbumin	1009:1035	the model antigen ovalbumin (Ova)	1009:1041	High protein-loading efficiency was obtained by testing with the model antigen ovalbumin (Ova), and the Ova adsorbed onto the cationic CS/O-HTCC complexes was taken up easily by the epithelium.
29713168	0	29	theme	quaternized	25:35	arg1	nanoparticles					46:58	Curdlan sulfate-O-linked quaternized chitosan nanoparticles	0:58	Curdlan sulfate-O-linked quaternized chitosan nanoparticles: potential adjuvants to improve the immunogenicity of exogenous antigens via intranasal vaccination.	0:159	Curdlan sulfate-O-linked quaternized chitosan nanoparticles: potential adjuvants to improve the immunogenicity of exogenous antigens via intranasal vaccination.
29713168	0	30	theme	intranasal	137:146	arg1	vaccination					148:158	intranasal vaccination	137:158	intranasal vaccination	137:158	Curdlan sulfate-O-linked quaternized chitosan nanoparticles: potential adjuvants to improve the immunogenicity of exogenous antigens via intranasal vaccination.
29713168	3	31	theme	chloride	673:680	arg1	nanoparticles					691:703	curdlan sulfate (CS)-O-(2-hydroxyl)propyl-3-trimethyl ammonium chitosan chloride (O-HTCC) nanoparticles	601:703	curdlan sulfate (CS)-O-(2-hydroxyl)propyl-3-trimethyl ammonium chitosan chloride (O-HTCC) nanoparticles	601:703	METHODS By using the advantages of polysaccharides, which can promote both T-helper 1 and 2 responses, curdlan sulfate (CS)-O-(2-hydroxyl)propyl-3-trimethyl ammonium chitosan chloride (O-HTCC) nanoparticles were prepared by interacting CS with O-HTCC, and the adjuvancy of the nanoparticles was investigated.
29713168	5	32	theme	antigen	1019:1025	arg1	Ova					1038:1040	Ova	1038:1040	Ova	1038:1040	High protein-loading efficiency was obtained by testing with the model antigen ovalbumin (Ova), and the Ova adsorbed onto the cationic CS/O-HTCC complexes was taken up easily by the epithelium.
29713168	5	32	theme	antigen	1019:1025	arg1	ovalbumin					1027:1035	the model antigen ovalbumin	1009:1035	the model antigen ovalbumin (Ova)	1009:1041	High protein-loading efficiency was obtained by testing with the model antigen ovalbumin (Ova), and the Ova adsorbed onto the cationic CS/O-HTCC complexes was taken up easily by the epithelium.
29713168	2	33	theme	mucosal	455:461	arg1	immunity					463:470	both systemic and mucosal immunity	437:470	both systemic and mucosal immunity	437:470	As an ideal intranasal vaccine adjuvant, it should have the properties of assisting soluble antigens to pass the mucosal barrier and potentiating both systemic and mucosal immunity via nasal administration.
29713168	9	34	theme	ideal	1774:1778	arg1	nanoparticles					1756:1768	CS/O-HTCC nanoparticles	1746:1768	CS/O-HTCC nanoparticles	1746:1768	CONCLUSION Therefore, these results suggest that CS/O-HTCC nanoparticles are ideal vaccine adjuvants for soluble antigens used in intranasal or mucosal vaccination.
29713168	9	34	theme	ideal	1774:1778	arg1	adjuvants					1788:1796	ideal vaccine adjuvants	1774:1796	ideal vaccine adjuvants for soluble antigens used in intranasal or mucosal vaccination	1774:1859	CONCLUSION Therefore, these results suggest that CS/O-HTCC nanoparticles are ideal vaccine adjuvants for soluble antigens used in intranasal or mucosal vaccination.
29713168	8	35	theme	higher	1656:1661	arg1	level					1663:1667	a significantly higher level	1640:1667	a significantly higher level of Ova-specific antibodies	1640:1694	Furthermore, CS/O-HTCC evoked a significantly higher level of Ova-specific antibodies.
29713168	9	36	theme	intranasal	1827:1836	arg1	vaccination					1849:1859	intranasal or mucosal vaccination	1827:1859	intranasal or mucosal vaccination	1827:1859	CONCLUSION Therefore, these results suggest that CS/O-HTCC nanoparticles are ideal vaccine adjuvants for soluble antigens used in intranasal or mucosal vaccination.
29713168	4	37	dep	RESULTS	807:813	arg1	results					819:825	The results	815:825	RESULTS The results	807:825	RESULTS The results showed that the polysaccharide-based nanoparticles induced the proliferation and activation of antigen-presenting cells.
29713168	8	38	theme	Ova-specific	1672:1683	arg1	antibodies					1685:1694	Ova-specific antibodies	1672:1694	Ova-specific antibodies	1672:1694	Furthermore, CS/O-HTCC evoked a significantly higher level of Ova-specific antibodies.
29713168	3	39	theme	ammonium	655:662	arg1	chloride					673:680	curdlan sulfate (CS)-O-(2-hydroxyl)propyl-3-trimethyl ammonium chitosan chloride	601:680	curdlan sulfate (CS)-O-(2-hydroxyl)propyl-3-trimethyl ammonium chitosan chloride (O-HTCC) nanoparticles	601:703	METHODS By using the advantages of polysaccharides, which can promote both T-helper 1 and 2 responses, curdlan sulfate (CS)-O-(2-hydroxyl)propyl-3-trimethyl ammonium chitosan chloride (O-HTCC) nanoparticles were prepared by interacting CS with O-HTCC, and the adjuvancy of the nanoparticles was investigated.
29713168	3	39	theme	ammonium	655:662	arg1	O-HTCC					683:688	O-HTCC	683:688	O-HTCC	683:688	METHODS By using the advantages of polysaccharides, which can promote both T-helper 1 and 2 responses, curdlan sulfate (CS)-O-(2-hydroxyl)propyl-3-trimethyl ammonium chitosan chloride (O-HTCC) nanoparticles were prepared by interacting CS with O-HTCC, and the adjuvancy of the nanoparticles was investigated.
29713168	2	40	theme	nasal	476:480	arg1	administration					482:495	nasal administration	476:495	nasal administration	476:495	As an ideal intranasal vaccine adjuvant, it should have the properties of assisting soluble antigens to pass the mucosal barrier and potentiating both systemic and mucosal immunity via nasal administration.
29713168	7	41	theme	cells	1448:1452	arg1	maturation					1415:1424	maturation	1415:1424	maturation	1415:1424	The results showed that Ova/CS/O-HTCC nanoparticles induced activation and maturation of antigen-presenting cells and provoked the proliferation and differentiation of lymphocytes more significantly compared to the immunization of Ova mixed with aluminum hydroxide gel.
29713168	7	41	theme	cells	1448:1452	arg1	activation					1400:1409	activation	1400:1409	activation	1400:1409	The results showed that Ova/CS/O-HTCC nanoparticles induced activation and maturation of antigen-presenting cells and provoked the proliferation and differentiation of lymphocytes more significantly compared to the immunization of Ova mixed with aluminum hydroxide gel.
29713168	2	42	theme	systemic	442:449	arg1	immunity					463:470	both systemic and mucosal immunity	437:470	both systemic and mucosal immunity	437:470	As an ideal intranasal vaccine adjuvant, it should have the properties of assisting soluble antigens to pass the mucosal barrier and potentiating both systemic and mucosal immunity via nasal administration.
29713168	2	43	contain	have	342:345	arg2	properties					351:360	the properties	347:360	the properties of assisting soluble antigens to pass the mucosal barrier and potentiating both systemic and mucosal immunity via nasal administration	347:495	As an ideal intranasal vaccine adjuvant, it should have the properties of assisting soluble antigens to pass the mucosal barrier and potentiating both systemic and mucosal immunity via nasal administration.
29713168	2	43	contain	have	342:345	arg2	vaccine					314:320	an ideal intranasal vaccine adjuvant	294:329	an ideal intranasal vaccine adjuvant	294:329	As an ideal intranasal vaccine adjuvant, it should have the properties of assisting soluble antigens to pass the mucosal barrier and potentiating both systemic and mucosal immunity via nasal administration.
29713168	2	43	contain	have	342:345	arg1	it					332:333	it	332:333	it	332:333	As an ideal intranasal vaccine adjuvant, it should have the properties of assisting soluble antigens to pass the mucosal barrier and potentiating both systemic and mucosal immunity via nasal administration.
29713168	9	44	theme	mucosal	1841:1847	arg1	vaccination					1849:1859	intranasal or mucosal vaccination	1827:1859	intranasal or mucosal vaccination	1827:1859	CONCLUSION Therefore, these results suggest that CS/O-HTCC nanoparticles are ideal vaccine adjuvants for soluble antigens used in intranasal or mucosal vaccination.
29713168	3	45	theme	chitosan	664:671	arg1	chloride					673:680	curdlan sulfate (CS)-O-(2-hydroxyl)propyl-3-trimethyl ammonium chitosan chloride	601:680	curdlan sulfate (CS)-O-(2-hydroxyl)propyl-3-trimethyl ammonium chitosan chloride (O-HTCC) nanoparticles	601:703	METHODS By using the advantages of polysaccharides, which can promote both T-helper 1 and 2 responses, curdlan sulfate (CS)-O-(2-hydroxyl)propyl-3-trimethyl ammonium chitosan chloride (O-HTCC) nanoparticles were prepared by interacting CS with O-HTCC, and the adjuvancy of the nanoparticles was investigated.
29713168	3	45	theme	chitosan	664:671	arg1	O-HTCC					683:688	O-HTCC	683:688	O-HTCC	683:688	METHODS By using the advantages of polysaccharides, which can promote both T-helper 1 and 2 responses, curdlan sulfate (CS)-O-(2-hydroxyl)propyl-3-trimethyl ammonium chitosan chloride (O-HTCC) nanoparticles were prepared by interacting CS with O-HTCC, and the adjuvancy of the nanoparticles was investigated.
29713168	7	46	theme	antigen-presenting	1429:1446	arg1	cells					1448:1452	antigen-presenting cells	1429:1452	antigen-presenting cells	1429:1452	The results showed that Ova/CS/O-HTCC nanoparticles induced activation and maturation of antigen-presenting cells and provoked the proliferation and differentiation of lymphocytes more significantly compared to the immunization of Ova mixed with aluminum hydroxide gel.
29713168	3	47	theme	polysaccharides	533:547	arg1	advantages					519:528	the advantages	515:528	the advantages of polysaccharides	515:547	METHODS By using the advantages of polysaccharides, which can promote both T-helper 1 and 2 responses, curdlan sulfate (CS)-O-(2-hydroxyl)propyl-3-trimethyl ammonium chitosan chloride (O-HTCC) nanoparticles were prepared by interacting CS with O-HTCC, and the adjuvancy of the nanoparticles was investigated.
29713168	0	48	theme	potential	61:69	arg1	adjuvants					71:79	potential adjuvants	61:79	potential adjuvants	61:79	Curdlan sulfate-O-linked quaternized chitosan nanoparticles: potential adjuvants to improve the immunogenicity of exogenous antigens via intranasal vaccination.
29713168	3	49	theme	nanoparticles	775:787	arg1	adjuvancy					758:766	the adjuvancy	754:766	the adjuvancy of the nanoparticles	754:787	METHODS By using the advantages of polysaccharides, which can promote both T-helper 1 and 2 responses, curdlan sulfate (CS)-O-(2-hydroxyl)propyl-3-trimethyl ammonium chitosan chloride (O-HTCC) nanoparticles were prepared by interacting CS with O-HTCC, and the adjuvancy of the nanoparticles was investigated.
29713168	7	50	theme	Ova/CS/O-HTCC	1364:1376	arg1	nanoparticles					1378:1390	Ova/CS/O-HTCC nanoparticles	1364:1390	Ova/CS/O-HTCC nanoparticles	1364:1390	The results showed that Ova/CS/O-HTCC nanoparticles induced activation and maturation of antigen-presenting cells and provoked the proliferation and differentiation of lymphocytes more significantly compared to the immunization of Ova mixed with aluminum hydroxide gel.
29713168	5	51	theme	CS/O-HTCC	1083:1091	arg1	complexes					1093:1101	the cationic CS/O-HTCC complexes	1070:1101	the cationic CS/O-HTCC complexes	1070:1101	High protein-loading efficiency was obtained by testing with the model antigen ovalbumin (Ova), and the Ova adsorbed onto the cationic CS/O-HTCC complexes was taken up easily by the epithelium.
29713168	4	52	theme	polysaccharide-based	843:862	arg1	nanoparticles					864:876	the polysaccharide-based nanoparticles	839:876	the polysaccharide-based nanoparticles	839:876	RESULTS The results showed that the polysaccharide-based nanoparticles induced the proliferation and activation of antigen-presenting cells.
29713168	9	53	theme	vaccine	1780:1786	arg1	nanoparticles					1756:1768	CS/O-HTCC nanoparticles	1746:1768	CS/O-HTCC nanoparticles	1746:1768	CONCLUSION Therefore, these results suggest that CS/O-HTCC nanoparticles are ideal vaccine adjuvants for soluble antigens used in intranasal or mucosal vaccination.
29713168	9	53	theme	vaccine	1780:1786	arg1	adjuvants					1788:1796	ideal vaccine adjuvants	1774:1796	ideal vaccine adjuvants for soluble antigens used in intranasal or mucosal vaccination	1774:1859	CONCLUSION Therefore, these results suggest that CS/O-HTCC nanoparticles are ideal vaccine adjuvants for soluble antigens used in intranasal or mucosal vaccination.
29713168	2	54	theme	soluble	375:381	arg1	antigens					383:390	soluble antigens	375:390	soluble antigens	375:390	As an ideal intranasal vaccine adjuvant, it should have the properties of assisting soluble antigens to pass the mucosal barrier and potentiating both systemic and mucosal immunity via nasal administration.
29713168	8	55	theme	antibodies	1685:1694	arg1	level					1663:1667	a significantly higher level	1640:1667	a significantly higher level of Ova-specific antibodies	1640:1694	Furthermore, CS/O-HTCC evoked a significantly higher level of Ova-specific antibodies.
29713168	3	56	dep	METHODS	498:504	arg1	using					509:513	using	509:513	using the advantages of polysaccharides	509:547	METHODS By using the advantages of polysaccharides, which can promote both T-helper 1 and 2 responses, curdlan sulfate (CS)-O-(2-hydroxyl)propyl-3-trimethyl ammonium chitosan chloride (O-HTCC) nanoparticles were prepared by interacting CS with O-HTCC, and the adjuvancy of the nanoparticles was investigated.
29713168	2	57	theme	intranasal	303:312	arg1	vaccine					314:320	an ideal intranasal vaccine adjuvant	294:329	an ideal intranasal vaccine adjuvant	294:329	As an ideal intranasal vaccine adjuvant, it should have the properties of assisting soluble antigens to pass the mucosal barrier and potentiating both systemic and mucosal immunity via nasal administration.
29713168	2	57	theme	intranasal	303:312	arg1	properties					351:360	the properties	347:360	the properties of assisting soluble antigens to pass the mucosal barrier and potentiating both systemic and mucosal immunity via nasal administration	347:495	As an ideal intranasal vaccine adjuvant, it should have the properties of assisting soluble antigens to pass the mucosal barrier and potentiating both systemic and mucosal immunity via nasal administration.
29713168	3	58	theme	-O-	621:623	arg1	chloride					673:680	curdlan sulfate (CS)-O-(2-hydroxyl)propyl-3-trimethyl ammonium chitosan chloride	601:680	curdlan sulfate (CS)-O-(2-hydroxyl)propyl-3-trimethyl ammonium chitosan chloride (O-HTCC) nanoparticles	601:703	METHODS By using the advantages of polysaccharides, which can promote both T-helper 1 and 2 responses, curdlan sulfate (CS)-O-(2-hydroxyl)propyl-3-trimethyl ammonium chitosan chloride (O-HTCC) nanoparticles were prepared by interacting CS with O-HTCC, and the adjuvancy of the nanoparticles was investigated.
29713168	3	58	theme	-O-	621:623	arg1	O-HTCC					683:688	O-HTCC	683:688	O-HTCC	683:688	METHODS By using the advantages of polysaccharides, which can promote both T-helper 1 and 2 responses, curdlan sulfate (CS)-O-(2-hydroxyl)propyl-3-trimethyl ammonium chitosan chloride (O-HTCC) nanoparticles were prepared by interacting CS with O-HTCC, and the adjuvancy of the nanoparticles was investigated.
29713168	7	59	theme	Ova	1571:1573	arg1	immunization					1555:1566	the immunization	1551:1566	the immunization of Ova mixed with aluminum hydroxide gel	1551:1607	The results showed that Ova/CS/O-HTCC nanoparticles induced activation and maturation of antigen-presenting cells and provoked the proliferation and differentiation of lymphocytes more significantly compared to the immunization of Ova mixed with aluminum hydroxide gel.
29713168	6	60	theme	nanoparticles	1188:1200	arg1	capacity					1158:1165	the capacity	1154:1165	the capacity of the Ova/CS/O-HTCC nanoparticles for immune enhancement	1154:1223	To evaluate the capacity of the Ova/CS/O-HTCC nanoparticles for immune enhancement in vivo, we collected and analyzed immunocytes, serum, and mucosal lavage fluid from intranasally vaccinated mice.
29713168	7	61	theme	lymphocytes	1508:1518	arg1	differentiation					1489:1503	differentiation	1489:1503	differentiation	1489:1503	The results showed that Ova/CS/O-HTCC nanoparticles induced activation and maturation of antigen-presenting cells and provoked the proliferation and differentiation of lymphocytes more significantly compared to the immunization of Ova mixed with aluminum hydroxide gel.
29713168	7	61	theme	lymphocytes	1508:1518	arg1	proliferation					1471:1483	proliferation	1471:1483	proliferation	1471:1483	The results showed that Ova/CS/O-HTCC nanoparticles induced activation and maturation of antigen-presenting cells and provoked the proliferation and differentiation of lymphocytes more significantly compared to the immunization of Ova mixed with aluminum hydroxide gel.
29713168	7	62	theme	aluminum	1586:1593	arg1	gel					1605:1607	aluminum hydroxide gel	1586:1607	aluminum hydroxide gel	1586:1607	The results showed that Ova/CS/O-HTCC nanoparticles induced activation and maturation of antigen-presenting cells and provoked the proliferation and differentiation of lymphocytes more significantly compared to the immunization of Ova mixed with aluminum hydroxide gel.
29713168	2	63	theme	ideal	297:301	arg1	vaccine					314:320	an ideal intranasal vaccine adjuvant	294:329	an ideal intranasal vaccine adjuvant	294:329	As an ideal intranasal vaccine adjuvant, it should have the properties of assisting soluble antigens to pass the mucosal barrier and potentiating both systemic and mucosal immunity via nasal administration.
29713168	2	63	theme	ideal	297:301	arg1	properties					351:360	the properties	347:360	the properties of assisting soluble antigens to pass the mucosal barrier and potentiating both systemic and mucosal immunity via nasal administration	347:495	As an ideal intranasal vaccine adjuvant, it should have the properties of assisting soluble antigens to pass the mucosal barrier and potentiating both systemic and mucosal immunity via nasal administration.
29713168	7	64	dep	proliferation	1471:1483	arg1	the					1467:1469	the	1467:1469	the	1467:1469	The results showed that Ova/CS/O-HTCC nanoparticles induced activation and maturation of antigen-presenting cells and provoked the proliferation and differentiation of lymphocytes more significantly compared to the immunization of Ova mixed with aluminum hydroxide gel.
29713168	1	65	theme	many	272:275	arg1	vaccinations					277:288	many vaccinations	272:288	many vaccinations	272:288	INTRODUCTION The development of ideal vaccine adjuvants for intranasal vaccination can provide convenience for many vaccinations.
29713168	7	66	theme	hydroxide	1595:1603	arg1	gel					1605:1607	aluminum hydroxide gel	1586:1607	aluminum hydroxide gel	1586:1607	The results showed that Ova/CS/O-HTCC nanoparticles induced activation and maturation of antigen-presenting cells and provoked the proliferation and differentiation of lymphocytes more significantly compared to the immunization of Ova mixed with aluminum hydroxide gel.
29713168	2	67	theme	mucosal	404:410	arg1	barrier					412:418	the mucosal barrier	400:418	the mucosal barrier	400:418	As an ideal intranasal vaccine adjuvant, it should have the properties of assisting soluble antigens to pass the mucosal barrier and potentiating both systemic and mucosal immunity via nasal administration.
29713168	6	68	from	mice	1334:1337	arg1	fluid					1299:1303	mucosal lavage fluid	1284:1303	mucosal lavage fluid	1284:1303	To evaluate the capacity of the Ova/CS/O-HTCC nanoparticles for immune enhancement in vivo, we collected and analyzed immunocytes, serum, and mucosal lavage fluid from intranasally vaccinated mice.
29713168	6	68	from	mice	1334:1337	arg1	serum					1273:1277	serum	1273:1277	serum	1273:1277	To evaluate the capacity of the Ova/CS/O-HTCC nanoparticles for immune enhancement in vivo, we collected and analyzed immunocytes, serum, and mucosal lavage fluid from intranasally vaccinated mice.
29713168	6	68	from	mice	1334:1337	arg1	immunocytes					1260:1270	immunocytes	1260:1270	immunocytes	1260:1270	To evaluate the capacity of the Ova/CS/O-HTCC nanoparticles for immune enhancement in vivo, we collected and analyzed immunocytes, serum, and mucosal lavage fluid from intranasally vaccinated mice.
29713168	3	69	theme	2-hydroxyl	625:634	arg1	chloride					673:680	curdlan sulfate (CS)-O-(2-hydroxyl)propyl-3-trimethyl ammonium chitosan chloride	601:680	curdlan sulfate (CS)-O-(2-hydroxyl)propyl-3-trimethyl ammonium chitosan chloride (O-HTCC) nanoparticles	601:703	METHODS By using the advantages of polysaccharides, which can promote both T-helper 1 and 2 responses, curdlan sulfate (CS)-O-(2-hydroxyl)propyl-3-trimethyl ammonium chitosan chloride (O-HTCC) nanoparticles were prepared by interacting CS with O-HTCC, and the adjuvancy of the nanoparticles was investigated.
29713168	3	69	theme	2-hydroxyl	625:634	arg1	O-HTCC					683:688	O-HTCC	683:688	O-HTCC	683:688	METHODS By using the advantages of polysaccharides, which can promote both T-helper 1 and 2 responses, curdlan sulfate (CS)-O-(2-hydroxyl)propyl-3-trimethyl ammonium chitosan chloride (O-HTCC) nanoparticles were prepared by interacting CS with O-HTCC, and the adjuvancy of the nanoparticles was investigated.
29713168	5	70	theme	High	948:951	arg1	efficiency					969:978	High protein-loading efficiency	948:978	High protein-loading efficiency	948:978	High protein-loading efficiency was obtained by testing with the model antigen ovalbumin (Ova), and the Ova adsorbed onto the cationic CS/O-HTCC complexes was taken up easily by the epithelium.
29713168	9	71	theme	CS/O-HTCC	1746:1754	arg1	nanoparticles					1756:1768	CS/O-HTCC nanoparticles	1746:1768	CS/O-HTCC nanoparticles	1746:1768	CONCLUSION Therefore, these results suggest that CS/O-HTCC nanoparticles are ideal vaccine adjuvants for soluble antigens used in intranasal or mucosal vaccination.
29713168	9	71	theme	CS/O-HTCC	1746:1754	arg1	adjuvants					1788:1796	ideal vaccine adjuvants	1774:1796	ideal vaccine adjuvants for soluble antigens used in intranasal or mucosal vaccination	1774:1859	CONCLUSION Therefore, these results suggest that CS/O-HTCC nanoparticles are ideal vaccine adjuvants for soluble antigens used in intranasal or mucosal vaccination.
31433728	0	0	theme	endoplasmic	92:102	arg1	reticulum					104:112	the endoplasmic reticulum	88:112	the endoplasmic reticulum in Saccharomyces cerevisiae	88:140	Asparagine-linked glycosylation is not directly coupled to protein translocation across the endoplasmic reticulum in Saccharomyces cerevisiae.
31433728	8	1	theme	site	1291:1294	arg1	occupation					1261:1270	occupation	1261:1270	occupation of the OST binding site by the Sec63 protein	1261:1315	A comparison of two recent protein structures indicates that the yeast OST is unable to interact with the yeast heptameric Sec complex via an evolutionarily conserved interface due to occupation of the OST binding site by the Sec63 protein.
31433728	8	2	theme	protein	1104:1110	arg1	structures					1112:1121	two recent protein structures	1093:1121	two recent protein structures	1093:1121	A comparison of two recent protein structures indicates that the yeast OST is unable to interact with the yeast heptameric Sec complex via an evolutionarily conserved interface due to occupation of the OST binding site by the Sec63 protein.
31433728	2	3	theme	N-terminal-to-C-terminal	399:422	arg1	mechanism					433:441	an N-terminal-to-C-terminal scanning mechanism	396:441	an N-terminal-to-C-terminal scanning mechanism	396:441	The STT3A complex interacts directly with the protein translocation channel to mediate glycosylation of proteins using an N-terminal-to-C-terminal scanning mechanism.
31433728	8	4	theme	binding	1283:1289	arg1	site					1291:1294	the OST binding site	1275:1294	the OST binding site	1275:1294	A comparison of two recent protein structures indicates that the yeast OST is unable to interact with the yeast heptameric Sec complex via an evolutionarily conserved interface due to occupation of the OST binding site by the Sec63 protein.
31433728	4	5	theme	glycoproteins	589:601	arg1	glycosylation					564:576	glycosylation	564:576	glycosylation of several glycoproteins in yeast and mammalian cells	564:630	We have compared glycosylation of several glycoproteins in yeast and mammalian cells.
31433728	5	6	theme	yeast	746:750	arg1	cells					752:756	yeast cells	746:756	yeast cells	746:756	Prosaposin, a cysteine-rich protein that contains STT3A-dependent glycosylation sites, is poorly glycosylated in yeast cells and STT3A-deficient human cells.
31433728	7	7	theme	reporter	1015:1022	arg1	proteins					1024:1031	carboxypeptidase Y-derived reporter proteins	988:1031	carboxypeptidase Y-derived reporter proteins that contain closely spaced acceptor sites	988:1074	Posttranslocational glycosylation was also observed for carboxypeptidase Y-derived reporter proteins that contain closely spaced acceptor sites.
31433728	4	8	theme	several	581:587	arg1	glycoproteins					589:601	several glycoproteins	581:601	several glycoproteins	581:601	We have compared glycosylation of several glycoproteins in yeast and mammalian cells.
31433728	7	9	contain	contain	1038:1044	arg2	sites					1070:1074	closely spaced acceptor sites	1046:1074	closely spaced acceptor sites	1046:1074	Posttranslocational glycosylation was also observed for carboxypeptidase Y-derived reporter proteins that contain closely spaced acceptor sites.
31433728	7	9	contain	contain	1038:1044	arg1	proteins					1024:1031	carboxypeptidase Y-derived reporter proteins	988:1031	carboxypeptidase Y-derived reporter proteins that contain closely spaced acceptor sites	988:1074	Posttranslocational glycosylation was also observed for carboxypeptidase Y-derived reporter proteins that contain closely spaced acceptor sites.
31433728	5	10	theme	STT3A-dependent	683:697	arg1	sites					713:717	STT3A-dependent glycosylation sites	683:717	STT3A-dependent glycosylation sites	683:717	Prosaposin, a cysteine-rich protein that contains STT3A-dependent glycosylation sites, is poorly glycosylated in yeast cells and STT3A-deficient human cells.
31433728	2	11	theme	protein	323:329	arg1	channel					345:351	the protein translocation channel	319:351	the protein translocation channel	319:351	The STT3A complex interacts directly with the protein translocation channel to mediate glycosylation of proteins using an N-terminal-to-C-terminal scanning mechanism.
31433728	6	12	gly	glycosylation	838:850	arg2	sites					852:856	extreme C-terminal glycosylation sites	819:856	extreme C-terminal glycosylation sites	819:856	In contrast, a protein with extreme C-terminal glycosylation sites was efficiently glycosylated in yeast by a posttranslocational mechanism.
31433728	6	13	with	protein	806:812	arg1	sites					852:856	extreme C-terminal glycosylation sites	819:856	extreme C-terminal glycosylation sites	819:856	In contrast, a protein with extreme C-terminal glycosylation sites was efficiently glycosylated in yeast by a posttranslocational mechanism.
31433728	9	14	theme	translocation	1431:1443	arg1	channels					1445:1452	the Sec61 or Ssh1 translocation channels	1413:1452	the Sec61 or Ssh1 translocation channels instead of the Sec complex	1413:1479	The efficiency of glycosylation in yeast is not enhanced for proteins that are translocated by the Sec61 or Ssh1 translocation channels instead of the Sec complex.
31433728	4	15	from	glycosylation	564:576	arg1	yeast					606:610	yeast	606:610	yeast	606:610	We have compared glycosylation of several glycoproteins in yeast and mammalian cells.
31433728	4	15	from	glycosylation	564:576	arg1	cells					626:630	mammalian cells	616:630	mammalian cells	616:630	We have compared glycosylation of several glycoproteins in yeast and mammalian cells.
31433728	5	16	theme	glycosylation	699:711	arg1	sites					713:717	STT3A-dependent glycosylation sites	683:717	STT3A-dependent glycosylation sites	683:717	Prosaposin, a cysteine-rich protein that contains STT3A-dependent glycosylation sites, is poorly glycosylated in yeast cells and STT3A-deficient human cells.
31433728	7	17	theme	Posttranslocational	932:950	arg1	glycosylation					952:964	Posttranslocational glycosylation	932:964	Posttranslocational glycosylation	932:964	Posttranslocational glycosylation was also observed for carboxypeptidase Y-derived reporter proteins that contain closely spaced acceptor sites.
31433728	4	18	gly	glycosylation	564:576	arg1	yeast					606:610	yeast	606:610	yeast	606:610	We have compared glycosylation of several glycoproteins in yeast and mammalian cells.
31433728	4	18	gly	glycosylation	564:576	arg1	cells					626:630	mammalian cells	616:630	mammalian cells	616:630	We have compared glycosylation of several glycoproteins in yeast and mammalian cells.
31433728	4	18	gly	glycosylation	564:576	arg1	glycoproteins					589:601	several glycoproteins	581:601	several glycoproteins	581:601	We have compared glycosylation of several glycoproteins in yeast and mammalian cells.
31433728	8	19	theme	yeast	1183:1187	arg1	complex					1204:1210	the yeast heptameric Sec complex	1179:1210	the yeast heptameric Sec complex	1179:1210	A comparison of two recent protein structures indicates that the yeast OST is unable to interact with the yeast heptameric Sec complex via an evolutionarily conserved interface due to occupation of the OST binding site by the Sec63 protein.
31433728	7	20	theme	carboxypeptidase	988:1003	arg1	proteins					1024:1031	carboxypeptidase Y-derived reporter proteins	988:1031	carboxypeptidase Y-derived reporter proteins that contain closely spaced acceptor sites	988:1074	Posttranslocational glycosylation was also observed for carboxypeptidase Y-derived reporter proteins that contain closely spaced acceptor sites.
31433728	8	21	theme	Sec	1200:1202	arg1	complex					1204:1210	the yeast heptameric Sec complex	1179:1210	the yeast heptameric Sec complex	1179:1210	A comparison of two recent protein structures indicates that the yeast OST is unable to interact with the yeast heptameric Sec complex via an evolutionarily conserved interface due to occupation of the OST binding site by the Sec63 protein.
31433728	0	22	link	Asparagine-linked	0:16	arg1	glycosylation					18:30	Asparagine-linked glycosylation	0:30	Asparagine-linked glycosylation	0:30	Asparagine-linked glycosylation is not directly coupled to protein translocation across the endoplasmic reticulum in Saccharomyces cerevisiae.
31433728	7	23	theme	Y-derived	1005:1013	arg1	proteins					1024:1031	carboxypeptidase Y-derived reporter proteins	988:1031	carboxypeptidase Y-derived reporter proteins that contain closely spaced acceptor sites	988:1074	Posttranslocational glycosylation was also observed for carboxypeptidase Y-derived reporter proteins that contain closely spaced acceptor sites.
31433728	0	24	theme	Saccharomyces	117:129	arg1	cerevisiae					131:140	Saccharomyces cerevisiae	117:140	Saccharomyces cerevisiae	117:140	Asparagine-linked glycosylation is not directly coupled to protein translocation across the endoplasmic reticulum in Saccharomyces cerevisiae.
31433728	3	25	from	glycosylation	453:465	arg1	yeast					490:494	budding yeast	482:494	budding yeast	482:494	N-linked glycosylation of proteins in budding yeast has been assumed to be a cotranslational reaction.
31433728	3	26	gly	glycosylation	453:465	arg1	proteins					470:477	proteins	470:477	proteins	470:477	N-linked glycosylation of proteins in budding yeast has been assumed to be a cotranslational reaction.
31433728	3	26	gly	glycosylation	453:465	arg1	yeast					490:494	budding yeast	482:494	budding yeast	482:494	N-linked glycosylation of proteins in budding yeast has been assumed to be a cotranslational reaction.
31433728	9	27	theme	glycosylation	1336:1348	arg1	efficiency					1322:1331	The efficiency	1318:1331	The efficiency of glycosylation in yeast	1318:1357	The efficiency of glycosylation in yeast is not enhanced for proteins that are translocated by the Sec61 or Ssh1 translocation channels instead of the Sec complex.
31433728	10	28	link	N-linked	1499:1506	arg1	glycosylation					1508:1520	N-linked glycosylation	1499:1520	N-linked glycosylation	1499:1520	We conclude that N-linked glycosylation and protein translocation are not directly coupled in yeast cells.
31433728	7	29	link	Y-derived	1005:1013	arg1	proteins					1024:1031	carboxypeptidase Y-derived reporter proteins	988:1031	carboxypeptidase Y-derived reporter proteins that contain closely spaced acceptor sites	988:1074	Posttranslocational glycosylation was also observed for carboxypeptidase Y-derived reporter proteins that contain closely spaced acceptor sites.
31433728	5	30	theme	cysteine-rich	647:659	arg1	protein					661:667	a cysteine-rich protein	645:667	a cysteine-rich protein that contains STT3A-dependent glycosylation sites	645:717	Prosaposin, a cysteine-rich protein that contains STT3A-dependent glycosylation sites, is poorly glycosylated in yeast cells and STT3A-deficient human cells.
31433728	5	30	theme	cysteine-rich	647:659	arg1	Prosaposin					633:642	Prosaposin	633:642	Prosaposin	633:642	Prosaposin, a cysteine-rich protein that contains STT3A-dependent glycosylation sites, is poorly glycosylated in yeast cells and STT3A-deficient human cells.
31433728	4	31	gly	glycoproteins	589:601	arg1	glycoproteins					589:601	several glycoproteins	581:601	several glycoproteins	581:601	We have compared glycosylation of several glycoproteins in yeast and mammalian cells.
31433728	0	32	theme	Asparagine-linked	0:16	arg1	glycosylation					18:30	Asparagine-linked glycosylation	0:30	Asparagine-linked glycosylation	0:30	Asparagine-linked glycosylation is not directly coupled to protein translocation across the endoplasmic reticulum in Saccharomyces cerevisiae.
31433728	10	33	gly	glycosylation	1508:1520	arg1	cells					1582:1586	yeast cells	1576:1586	yeast cells	1576:1586	We conclude that N-linked glycosylation and protein translocation are not directly coupled in yeast cells.
31433728	5	34	contain	contains	674:681	arg1	protein					661:667	a cysteine-rich protein	645:667	a cysteine-rich protein that contains STT3A-dependent glycosylation sites	645:717	Prosaposin, a cysteine-rich protein that contains STT3A-dependent glycosylation sites, is poorly glycosylated in yeast cells and STT3A-deficient human cells.
31433728	5	34	contain	contains	674:681	arg2	sites					713:717	STT3A-dependent glycosylation sites	683:717	STT3A-dependent glycosylation sites	683:717	Prosaposin, a cysteine-rich protein that contains STT3A-dependent glycosylation sites, is poorly glycosylated in yeast cells and STT3A-deficient human cells.
31433728	5	34	contain	contains	674:681	arg1	Prosaposin					633:642	Prosaposin	633:642	Prosaposin	633:642	Prosaposin, a cysteine-rich protein that contains STT3A-dependent glycosylation sites, is poorly glycosylated in yeast cells and STT3A-deficient human cells.
31433728	9	35	theme	Sec	1469:1471	arg1	complex					1473:1479	the Sec complex	1465:1479	the Sec complex	1465:1479	The efficiency of glycosylation in yeast is not enhanced for proteins that are translocated by the Sec61 or Ssh1 translocation channels instead of the Sec complex.
31433728	10	36	theme	yeast	1576:1580	arg1	cells					1582:1586	yeast cells	1576:1586	yeast cells	1576:1586	We conclude that N-linked glycosylation and protein translocation are not directly coupled in yeast cells.
31433728	1	37	link	N-linked	253:260	arg1	glycosylation					262:274	N-linked glycosylation	253:274	N-linked glycosylation	253:274	Mammalian cells express two oligosaccharyltransferase complexes, STT3A and STT3B, that have distinct roles in N-linked glycosylation.
31433728	9	38	theme	complex	1473:1479	arg1	channels					1445:1452	the Sec61 or Ssh1 translocation channels	1413:1452	the Sec61 or Ssh1 translocation channels instead of the Sec complex	1413:1479	The efficiency of glycosylation in yeast is not enhanced for proteins that are translocated by the Sec61 or Ssh1 translocation channels instead of the Sec complex.
31433728	3	39	theme	cotranslational	521:535	arg1	glycosylation					453:465	N-linked glycosylation	444:465	N-linked glycosylation of proteins in budding yeast	444:494	N-linked glycosylation of proteins in budding yeast has been assumed to be a cotranslational reaction.
31433728	3	39	theme	cotranslational	521:535	arg1	reaction					537:544	a cotranslational reaction	519:544	a cotranslational reaction	519:544	N-linked glycosylation of proteins in budding yeast has been assumed to be a cotranslational reaction.
31433728	3	40	theme	N-linked	444:451	arg1	glycosylation					453:465	N-linked glycosylation	444:465	N-linked glycosylation of proteins in budding yeast	444:494	N-linked glycosylation of proteins in budding yeast has been assumed to be a cotranslational reaction.
31433728	3	40	theme	N-linked	444:451	arg1	reaction					537:544	a cotranslational reaction	519:544	a cotranslational reaction	519:544	N-linked glycosylation of proteins in budding yeast has been assumed to be a cotranslational reaction.
31433728	6	41	gly	glycosylated	874:885	arg1	yeast					890:894	yeast	890:894	yeast	890:894	In contrast, a protein with extreme C-terminal glycosylation sites was efficiently glycosylated in yeast by a posttranslocational mechanism.
31433728	6	41	gly	glycosylated	874:885	arg1	contrast					794:801	contrast	794:801	contrast	794:801	In contrast, a protein with extreme C-terminal glycosylation sites was efficiently glycosylated in yeast by a posttranslocational mechanism.
31433728	6	41	gly	glycosylated	874:885	arg1	protein					806:812	a protein	804:812	a protein with extreme C-terminal glycosylation sites	804:856	In contrast, a protein with extreme C-terminal glycosylation sites was efficiently glycosylated in yeast by a posttranslocational mechanism.
31433728	6	41	gly	glycosylated	874:885	arg2	protein					806:812	a protein	804:812	a protein with extreme C-terminal glycosylation sites	804:856	In contrast, a protein with extreme C-terminal glycosylation sites was efficiently glycosylated in yeast by a posttranslocational mechanism.
31433728	2	42	theme	translocation	331:343	arg1	channel					345:351	the protein translocation channel	319:351	the protein translocation channel	319:351	The STT3A complex interacts directly with the protein translocation channel to mediate glycosylation of proteins using an N-terminal-to-C-terminal scanning mechanism.
31433728	6	43	theme	posttranslocational	901:919	arg1	mechanism					921:929	a posttranslocational mechanism	899:929	a posttranslocational mechanism	899:929	In contrast, a protein with extreme C-terminal glycosylation sites was efficiently glycosylated in yeast by a posttranslocational mechanism.
31433728	3	44	theme	budding	482:488	arg1	yeast					490:494	budding yeast	482:494	budding yeast	482:494	N-linked glycosylation of proteins in budding yeast has been assumed to be a cotranslational reaction.
31433728	2	45	theme	proteins	381:388	arg1	glycosylation					364:376	glycosylation	364:376	glycosylation of proteins using an N-terminal-to-C-terminal scanning mechanism	364:441	The STT3A complex interacts directly with the protein translocation channel to mediate glycosylation of proteins using an N-terminal-to-C-terminal scanning mechanism.
31433728	5	46	gly	glycosylated	730:741	arg1	protein					661:667	a cysteine-rich protein	645:667	a cysteine-rich protein that contains STT3A-dependent glycosylation sites	645:717	Prosaposin, a cysteine-rich protein that contains STT3A-dependent glycosylation sites, is poorly glycosylated in yeast cells and STT3A-deficient human cells.
31433728	5	46	gly	glycosylated	730:741	arg1	Prosaposin					633:642	Prosaposin	633:642	Prosaposin	633:642	Prosaposin, a cysteine-rich protein that contains STT3A-dependent glycosylation sites, is poorly glycosylated in yeast cells and STT3A-deficient human cells.
31433728	5	46	gly	glycosylated	730:741	arg1	cells					784:788	STT3A-deficient human cells	762:788	STT3A-deficient human cells	762:788	Prosaposin, a cysteine-rich protein that contains STT3A-dependent glycosylation sites, is poorly glycosylated in yeast cells and STT3A-deficient human cells.
31433728	5	46	gly	glycosylated	730:741	arg2	protein					661:667	a cysteine-rich protein	645:667	a cysteine-rich protein that contains STT3A-dependent glycosylation sites	645:717	Prosaposin, a cysteine-rich protein that contains STT3A-dependent glycosylation sites, is poorly glycosylated in yeast cells and STT3A-deficient human cells.
31433728	5	46	gly	glycosylated	730:741	arg2	Prosaposin					633:642	Prosaposin	633:642	Prosaposin	633:642	Prosaposin, a cysteine-rich protein that contains STT3A-dependent glycosylation sites, is poorly glycosylated in yeast cells and STT3A-deficient human cells.
31433728	5	46	gly	glycosylated	730:741	arg1	cells					752:756	yeast cells	746:756	yeast cells	746:756	Prosaposin, a cysteine-rich protein that contains STT3A-dependent glycosylation sites, is poorly glycosylated in yeast cells and STT3A-deficient human cells.
31433728	1	47	theme	distinct	235:242	arg1	roles					244:248	distinct roles	235:248	distinct roles	235:248	Mammalian cells express two oligosaccharyltransferase complexes, STT3A and STT3B, that have distinct roles in N-linked glycosylation.
31433728	8	48	theme	yeast	1142:1146	arg1	unable					1155:1160	unable	1155:1160	unable	1155:1160	A comparison of two recent protein structures indicates that the yeast OST is unable to interact with the yeast heptameric Sec complex via an evolutionarily conserved interface due to occupation of the OST binding site by the Sec63 protein.
31433728	8	48	theme	yeast	1142:1146	arg1	OST					1148:1150	the yeast OST	1138:1150	the yeast OST	1138:1150	A comparison of two recent protein structures indicates that the yeast OST is unable to interact with the yeast heptameric Sec complex via an evolutionarily conserved interface due to occupation of the OST binding site by the Sec63 protein.
31433728	8	49	theme	conserved	1234:1242	arg1	interface					1244:1252	an evolutionarily conserved interface	1216:1252	an evolutionarily conserved interface due to occupation of the OST binding site by the Sec63 protein	1216:1315	A comparison of two recent protein structures indicates that the yeast OST is unable to interact with the yeast heptameric Sec complex via an evolutionarily conserved interface due to occupation of the OST binding site by the Sec63 protein.
31433728	8	50	theme	Sec63	1303:1307	arg1	protein					1309:1315	the Sec63 protein	1299:1315	the Sec63 protein	1299:1315	A comparison of two recent protein structures indicates that the yeast OST is unable to interact with the yeast heptameric Sec complex via an evolutionarily conserved interface due to occupation of the OST binding site by the Sec63 protein.
31433728	0	51	theme	protein	59:65	arg1	translocation					67:79	protein translocation	59:79	protein translocation across the endoplasmic reticulum in Saccharomyces cerevisiae	59:140	Asparagine-linked glycosylation is not directly coupled to protein translocation across the endoplasmic reticulum in Saccharomyces cerevisiae.
31433728	5	52	theme	STT3A-deficient	762:776	arg1	cells					784:788	STT3A-deficient human cells	762:788	STT3A-deficient human cells	762:788	Prosaposin, a cysteine-rich protein that contains STT3A-dependent glycosylation sites, is poorly glycosylated in yeast cells and STT3A-deficient human cells.
31433728	10	53	theme	protein	1526:1532	arg1	translocation					1534:1546	protein translocation	1526:1546	protein translocation	1526:1546	We conclude that N-linked glycosylation and protein translocation are not directly coupled in yeast cells.
31433728	10	54	theme	N-linked	1499:1506	arg1	glycosylation					1508:1520	N-linked glycosylation	1499:1520	N-linked glycosylation	1499:1520	We conclude that N-linked glycosylation and protein translocation are not directly coupled in yeast cells.
31433728	9	55	theme	Ssh1	1426:1429	arg1	translocation					1431:1443	Ssh1 translocation	1426:1443	Ssh1 translocation	1426:1443	The efficiency of glycosylation in yeast is not enhanced for proteins that are translocated by the Sec61 or Ssh1 translocation channels instead of the Sec complex.
31433728	4	56	theme	mammalian	616:624	arg1	cells					626:630	mammalian cells	616:630	mammalian cells	616:630	We have compared glycosylation of several glycoproteins in yeast and mammalian cells.
31433728	5	57	theme	human	778:782	arg1	cells					784:788	STT3A-deficient human cells	762:788	STT3A-deficient human cells	762:788	Prosaposin, a cysteine-rich protein that contains STT3A-dependent glycosylation sites, is poorly glycosylated in yeast cells and STT3A-deficient human cells.
31433728	8	58	theme	heptameric	1189:1198	arg1	complex					1204:1210	the yeast heptameric Sec complex	1179:1210	the yeast heptameric Sec complex	1179:1210	A comparison of two recent protein structures indicates that the yeast OST is unable to interact with the yeast heptameric Sec complex via an evolutionarily conserved interface due to occupation of the OST binding site by the Sec63 protein.
31433728	9	59	theme	Sec61	1417:1421	arg1	channels					1445:1452	the Sec61 or Ssh1 translocation channels	1413:1452	the Sec61 or Ssh1 translocation channels instead of the Sec complex	1413:1479	The efficiency of glycosylation in yeast is not enhanced for proteins that are translocated by the Sec61 or Ssh1 translocation channels instead of the Sec complex.
31433728	1	60	theme	Mammalian	143:151	arg1	cells					153:157	Mammalian cells	143:157	Mammalian cells	143:157	Mammalian cells express two oligosaccharyltransferase complexes, STT3A and STT3B, that have distinct roles in N-linked glycosylation.
31433728	7	61	theme	acceptor	1061:1068	arg1	sites					1070:1074	closely spaced acceptor sites	1046:1074	closely spaced acceptor sites	1046:1074	Posttranslocational glycosylation was also observed for carboxypeptidase Y-derived reporter proteins that contain closely spaced acceptor sites.
31433728	2	62	gly	glycosylation	364:376	arg1	proteins					381:388	proteins	381:388	proteins using an N-terminal-to-C-terminal scanning mechanism	381:441	The STT3A complex interacts directly with the protein translocation channel to mediate glycosylation of proteins using an N-terminal-to-C-terminal scanning mechanism.
31433728	6	63	theme	glycosylation	838:850	arg1	sites					852:856	extreme C-terminal glycosylation sites	819:856	extreme C-terminal glycosylation sites	819:856	In contrast, a protein with extreme C-terminal glycosylation sites was efficiently glycosylated in yeast by a posttranslocational mechanism.
31433728	1	64	contain	have	230:233	arg2	roles					244:248	distinct roles	235:248	distinct roles	235:248	Mammalian cells express two oligosaccharyltransferase complexes, STT3A and STT3B, that have distinct roles in N-linked glycosylation.
31433728	1	64	contain	have	230:233	arg1	STT3B					218:222	STT3B	218:222	STT3B	218:222	Mammalian cells express two oligosaccharyltransferase complexes, STT3A and STT3B, that have distinct roles in N-linked glycosylation.
31433728	1	64	contain	have	230:233	arg1	STT3A					208:212	STT3A	208:212	STT3A	208:212	Mammalian cells express two oligosaccharyltransferase complexes, STT3A and STT3B, that have distinct roles in N-linked glycosylation.
31433728	1	64	contain	have	230:233	arg1	complexes					197:205	two oligosaccharyltransferase complexes	167:205	two oligosaccharyltransferase complexes	167:205	Mammalian cells express two oligosaccharyltransferase complexes, STT3A and STT3B, that have distinct roles in N-linked glycosylation.
31433728	3	65	link	N-linked	444:451	arg1	glycosylation					453:465	N-linked glycosylation	444:465	N-linked glycosylation of proteins in budding yeast	444:494	N-linked glycosylation of proteins in budding yeast has been assumed to be a cotranslational reaction.
31433728	3	65	link	N-linked	444:451	arg1	reaction					537:544	a cotranslational reaction	519:544	a cotranslational reaction	519:544	N-linked glycosylation of proteins in budding yeast has been assumed to be a cotranslational reaction.
31433728	6	66	theme	C-terminal	827:836	arg1	sites					852:856	extreme C-terminal glycosylation sites	819:856	extreme C-terminal glycosylation sites	819:856	In contrast, a protein with extreme C-terminal glycosylation sites was efficiently glycosylated in yeast by a posttranslocational mechanism.
31433728	1	67	theme	oligosaccharyltransferase	171:195	arg1	STT3B					218:222	STT3B	218:222	STT3B	218:222	Mammalian cells express two oligosaccharyltransferase complexes, STT3A and STT3B, that have distinct roles in N-linked glycosylation.
31433728	1	67	theme	oligosaccharyltransferase	171:195	arg1	STT3A					208:212	STT3A	208:212	STT3A	208:212	Mammalian cells express two oligosaccharyltransferase complexes, STT3A and STT3B, that have distinct roles in N-linked glycosylation.
31433728	1	67	theme	oligosaccharyltransferase	171:195	arg1	complexes					197:205	two oligosaccharyltransferase complexes	167:205	two oligosaccharyltransferase complexes	167:205	Mammalian cells express two oligosaccharyltransferase complexes, STT3A and STT3B, that have distinct roles in N-linked glycosylation.
31433728	1	68	theme	N-linked	253:260	arg1	glycosylation					262:274	N-linked glycosylation	253:274	N-linked glycosylation	253:274	Mammalian cells express two oligosaccharyltransferase complexes, STT3A and STT3B, that have distinct roles in N-linked glycosylation.
31433728	8	69	theme	OST	1279:1281	arg1	site					1291:1294	the OST binding site	1275:1294	the OST binding site	1275:1294	A comparison of two recent protein structures indicates that the yeast OST is unable to interact with the yeast heptameric Sec complex via an evolutionarily conserved interface due to occupation of the OST binding site by the Sec63 protein.
31433728	0	70	from	reticulum	104:112	arg1	cerevisiae					131:140	Saccharomyces cerevisiae	117:140	Saccharomyces cerevisiae	117:140	Asparagine-linked glycosylation is not directly coupled to protein translocation across the endoplasmic reticulum in Saccharomyces cerevisiae.
31433728	2	71	theme	STT3A	281:285	arg1	complex					287:293	The STT3A complex	277:293	The STT3A complex	277:293	The STT3A complex interacts directly with the protein translocation channel to mediate glycosylation of proteins using an N-terminal-to-C-terminal scanning mechanism.
31433728	6	72	theme	extreme	819:825	arg1	sites					852:856	extreme C-terminal glycosylation sites	819:856	extreme C-terminal glycosylation sites	819:856	In contrast, a protein with extreme C-terminal glycosylation sites was efficiently glycosylated in yeast by a posttranslocational mechanism.
31433728	9	73	from	efficiency	1322:1331	arg1	yeast					1353:1357	yeast	1353:1357	yeast	1353:1357	The efficiency of glycosylation in yeast is not enhanced for proteins that are translocated by the Sec61 or Ssh1 translocation channels instead of the Sec complex.
31433728	7	74	theme	spaced	1054:1059	arg1	sites					1070:1074	closely spaced acceptor sites	1046:1074	closely spaced acceptor sites	1046:1074	Posttranslocational glycosylation was also observed for carboxypeptidase Y-derived reporter proteins that contain closely spaced acceptor sites.
31433728	3	75	theme	proteins	470:477	arg1	glycosylation					453:465	N-linked glycosylation	444:465	N-linked glycosylation of proteins in budding yeast	444:494	N-linked glycosylation of proteins in budding yeast has been assumed to be a cotranslational reaction.
31433728	3	75	theme	proteins	470:477	arg1	reaction					537:544	a cotranslational reaction	519:544	a cotranslational reaction	519:544	N-linked glycosylation of proteins in budding yeast has been assumed to be a cotranslational reaction.
31433728	8	76	theme	recent	1097:1102	arg1	structures					1112:1121	two recent protein structures	1093:1121	two recent protein structures	1093:1121	A comparison of two recent protein structures indicates that the yeast OST is unable to interact with the yeast heptameric Sec complex via an evolutionarily conserved interface due to occupation of the OST binding site by the Sec63 protein.
31433728	8	77	theme	structures	1112:1121	arg1	comparison					1079:1088	A comparison	1077:1088	A comparison of two recent protein structures	1077:1121	A comparison of two recent protein structures indicates that the yeast OST is unable to interact with the yeast heptameric Sec complex via an evolutionarily conserved interface due to occupation of the OST binding site by the Sec63 protein.
31433728	2	78	theme	scanning	424:431	arg1	mechanism					433:441	an N-terminal-to-C-terminal scanning mechanism	396:441	an N-terminal-to-C-terminal scanning mechanism	396:441	The STT3A complex interacts directly with the protein translocation channel to mediate glycosylation of proteins using an N-terminal-to-C-terminal scanning mechanism.
31433728	5	79	gly	glycosylation	699:711	arg2	sites					713:717	STT3A-dependent glycosylation sites	683:717	STT3A-dependent glycosylation sites	683:717	Prosaposin, a cysteine-rich protein that contains STT3A-dependent glycosylation sites, is poorly glycosylated in yeast cells and STT3A-deficient human cells.
31042459	6	0	theme	B-cell	817:822	arg1	leg-type					834:841	leg-type	834:841	leg-type	834:841	Full-length functional variable, diversity, and joining gene sequences of 18 PCFCL and 8 primary cutaneous diffuse large B-cell lymphoma, leg-type were identified by unbiased Anchoring Reverse Transcription of Immunoglobulin Sequences and Amplification by Nested PCR and BCR reconstruction from RNA sequencing data.
31042459	6	0	theme	B-cell	817:822	arg1	lymphoma					824:831	cutaneous diffuse large B-cell lymphoma	793:831	8 primary cutaneous diffuse large B-cell lymphoma	783:831	Full-length functional variable, diversity, and joining gene sequences of 18 PCFCL and 8 primary cutaneous diffuse large B-cell lymphoma, leg-type were identified by unbiased Anchoring Reverse Transcription of Immunoglobulin Sequences and Amplification by Nested PCR and BCR reconstruction from RNA sequencing data.
31042459	6	1	theme	Sequences	921:929	arg1	Amplification					935:947	Amplification	935:947	Amplification	935:947	Full-length functional variable, diversity, and joining gene sequences of 18 PCFCL and 8 primary cutaneous diffuse large B-cell lymphoma, leg-type were identified by unbiased Anchoring Reverse Transcription of Immunoglobulin Sequences and Amplification by Nested PCR and BCR reconstruction from RNA sequencing data.
31042459	6	1	theme	Sequences	921:929	arg1	Transcription					889:901	unbiased Anchoring Reverse Transcription	862:901	unbiased Anchoring Reverse Transcription of Immunoglobulin Sequences	862:929	Full-length functional variable, diversity, and joining gene sequences of 18 PCFCL and 8 primary cutaneous diffuse large B-cell lymphoma, leg-type were identified by unbiased Anchoring Reverse Transcription of Immunoglobulin Sequences and Amplification by Nested PCR and BCR reconstruction from RNA sequencing data.
31042459	4	2	theme	FL	595:596	arg1	pathogenesis					598:609	FL pathogenesis	595:609	FL pathogenesis	595:609	The stimulation of mannosylated BCR by lectins on the tumor microenvironment is therefore a candidate driver in FL pathogenesis.
31042459	8	3	from	contrast	1270:1277	arg1	Similar					1249:1255	Similar	1249:1255	Similar	1249:1255	Similar to FL but in contrast to primary cutaneous diffuse large B-cell lymphoma, leg-type, BCR genes of 15 PCFCLs (83%) had acquired N-linked glycosylation motifs.
31042459	6	4	theme	diffuse	803:809	arg1	leg-type					834:841	leg-type	834:841	leg-type	834:841	Full-length functional variable, diversity, and joining gene sequences of 18 PCFCL and 8 primary cutaneous diffuse large B-cell lymphoma, leg-type were identified by unbiased Anchoring Reverse Transcription of Immunoglobulin Sequences and Amplification by Nested PCR and BCR reconstruction from RNA sequencing data.
31042459	6	4	theme	diffuse	803:809	arg1	lymphoma					824:831	cutaneous diffuse large B-cell lymphoma	793:831	8 primary cutaneous diffuse large B-cell lymphoma	783:831	Full-length functional variable, diversity, and joining gene sequences of 18 PCFCL and 8 primary cutaneous diffuse large B-cell lymphoma, leg-type were identified by unbiased Anchoring Reverse Transcription of Immunoglobulin Sequences and Amplification by Nested PCR and BCR reconstruction from RNA sequencing data.
31042459	6	5	theme	primary	785:791	arg1	leg-type					834:841	leg-type	834:841	leg-type	834:841	Full-length functional variable, diversity, and joining gene sequences of 18 PCFCL and 8 primary cutaneous diffuse large B-cell lymphoma, leg-type were identified by unbiased Anchoring Reverse Transcription of Immunoglobulin Sequences and Amplification by Nested PCR and BCR reconstruction from RNA sequencing data.
31042459	6	5	theme	primary	785:791	arg1	lymphoma					824:831	cutaneous diffuse large B-cell lymphoma	793:831	8 primary cutaneous diffuse large B-cell lymphoma	783:831	Full-length functional variable, diversity, and joining gene sequences of 18 PCFCL and 8 primary cutaneous diffuse large B-cell lymphoma, leg-type were identified by unbiased Anchoring Reverse Transcription of Immunoglobulin Sequences and Amplification by Nested PCR and BCR reconstruction from RNA sequencing data.
31042459	6	6	theme	Reverse	881:887	arg1	Transcription					889:901	unbiased Anchoring Reverse Transcription	862:901	unbiased Anchoring Reverse Transcription of Immunoglobulin Sequences	862:929	Full-length functional variable, diversity, and joining gene sequences of 18 PCFCL and 8 primary cutaneous diffuse large B-cell lymphoma, leg-type were identified by unbiased Anchoring Reverse Transcription of Immunoglobulin Sequences and Amplification by Nested PCR and BCR reconstruction from RNA sequencing data.
31042459	7	7	theme	B-cell	1129:1134	arg1	lymphoma					1136:1143	primary cutaneous diffuse large B-cell lymphoma	1097:1143	primary cutaneous diffuse large B-cell lymphoma	1097:1143	Low BCR variation demonstrated negligible ongoing somatic hypermutation in PCFCL and primary cutaneous diffuse large B-cell lymphoma, leg-type, and indicated that the PCFCL microarchitecture does not act as a functional germinal center.
31042459	6	8	theme	RNA	991:993	arg1	data					1006:1009	RNA sequencing data	991:1009	RNA sequencing data	991:1009	Full-length functional variable, diversity, and joining gene sequences of 18 PCFCL and 8 primary cutaneous diffuse large B-cell lymphoma, leg-type were identified by unbiased Anchoring Reverse Transcription of Immunoglobulin Sequences and Amplification by Nested PCR and BCR reconstruction from RNA sequencing data.
31042459	8	9	theme	cutaneous	1290:1298	arg1	leg-type					1331:1338	leg-type	1331:1338	leg-type	1331:1338	Similar to FL but in contrast to primary cutaneous diffuse large B-cell lymphoma, leg-type, BCR genes of 15 PCFCLs (83%) had acquired N-linked glycosylation motifs.
31042459	8	9	theme	cutaneous	1290:1298	arg1	lymphoma					1321:1328	primary cutaneous diffuse large B-cell lymphoma	1282:1328	primary cutaneous diffuse large B-cell lymphoma	1282:1328	Similar to FL but in contrast to primary cutaneous diffuse large B-cell lymphoma, leg-type, BCR genes of 15 PCFCLs (83%) had acquired N-linked glycosylation motifs.
31042459	7	10	theme	diffuse	1115:1121	arg1	lymphoma					1136:1143	primary cutaneous diffuse large B-cell lymphoma	1097:1143	primary cutaneous diffuse large B-cell lymphoma	1097:1143	Low BCR variation demonstrated negligible ongoing somatic hypermutation in PCFCL and primary cutaneous diffuse large B-cell lymphoma, leg-type, and indicated that the PCFCL microarchitecture does not act as a functional germinal center.
31042459	1	11	theme	mature	191:196	arg1	lymphoma					164:171	Primary cutaneous follicle center lymphoma	130:171	Primary cutaneous follicle center lymphoma (PCFCL)	130:179	Primary cutaneous follicle center lymphoma (PCFCL) is a rare mature B-cell lymphoma with an unknown etiology.
31042459	1	11	theme	mature	191:196	arg1	lymphoma					205:212	a rare mature B-cell lymphoma	184:212	a rare mature B-cell lymphoma with an unknown etiology	184:237	Primary cutaneous follicle center lymphoma (PCFCL) is a rare mature B-cell lymphoma with an unknown etiology.
31042459	8	12	theme	large	1308:1312	arg1	leg-type					1331:1338	leg-type	1331:1338	leg-type	1331:1338	Similar to FL but in contrast to primary cutaneous diffuse large B-cell lymphoma, leg-type, BCR genes of 15 PCFCLs (83%) had acquired N-linked glycosylation motifs.
31042459	8	12	theme	large	1308:1312	arg1	lymphoma					1321:1328	primary cutaneous diffuse large B-cell lymphoma	1282:1328	primary cutaneous diffuse large B-cell lymphoma	1282:1328	Similar to FL but in contrast to primary cutaneous diffuse large B-cell lymphoma, leg-type, BCR genes of 15 PCFCLs (83%) had acquired N-linked glycosylation motifs.
31042459	7	13	theme	primary	1097:1103	arg1	lymphoma					1136:1143	primary cutaneous diffuse large B-cell lymphoma	1097:1143	primary cutaneous diffuse large B-cell lymphoma	1097:1143	Low BCR variation demonstrated negligible ongoing somatic hypermutation in PCFCL and primary cutaneous diffuse large B-cell lymphoma, leg-type, and indicated that the PCFCL microarchitecture does not act as a functional germinal center.
31042459	7	14	theme	negligible	1043:1052	arg1	hypermutation					1070:1082	negligible ongoing somatic hypermutation	1043:1082	negligible ongoing somatic hypermutation	1043:1082	Low BCR variation demonstrated negligible ongoing somatic hypermutation in PCFCL and primary cutaneous diffuse large B-cell lymphoma, leg-type, and indicated that the PCFCL microarchitecture does not act as a functional germinal center.
31042459	9	15	theme	glycosylation	1483:1495	arg1	motifs					1497:1502	N-linked glycosylation motifs	1474:1502	N-linked glycosylation motifs in normal B-cell repertoires	1474:1531	These motifs were located at the BCR positions converted to N-linked glycosylation motifs in normal B-cell repertoires with low prevalence but mostly at different positions than those found in FL.
31042459	10	16	theme	cutaneous	1615:1623	arg1	localization					1625:1636	The cutaneous localization	1611:1636	The cutaneous localization of PCFCL	1611:1645	The cutaneous localization of PCFCL might suggest a role for lectins from commensal skin bacteria in PCFCL lymphomagenesis.
31042459	5	17	theme	PCFCL	676:680	arg1	pathogenesis					682:693	PCFCL pathogenesis	676:693	PCFCL pathogenesis	676:693	We investigated whether the same mechanism could play a role in PCFCL pathogenesis.
31042459	6	18	theme	Nested	952:957	arg1	PCR					959:961	Nested PCR	952:961	Nested PCR	952:961	Full-length functional variable, diversity, and joining gene sequences of 18 PCFCL and 8 primary cutaneous diffuse large B-cell lymphoma, leg-type were identified by unbiased Anchoring Reverse Transcription of Immunoglobulin Sequences and Amplification by Nested PCR and BCR reconstruction from RNA sequencing data.
31042459	2	19	theme	follicular	256:265	arg1	FL					277:278	FL	277:278	FL	277:278	PCFCL resembles follicular lymphoma (FL) by cytomorphologic and microarchitectural criteria.
31042459	2	19	theme	follicular	256:265	arg1	lymphoma					267:274	follicular lymphoma	256:274	follicular lymphoma (FL)	256:279	PCFCL resembles follicular lymphoma (FL) by cytomorphologic and microarchitectural criteria.
31042459	7	20	theme	Low	1012:1014	arg1	variation					1020:1028	Low BCR variation	1012:1028	Low BCR variation	1012:1028	Low BCR variation demonstrated negligible ongoing somatic hypermutation in PCFCL and primary cutaneous diffuse large B-cell lymphoma, leg-type, and indicated that the PCFCL microarchitecture does not act as a functional germinal center.
31042459	1	21	theme	cutaneous	138:146	arg1	PCFCL					174:178	PCFCL	174:178	PCFCL	174:178	Primary cutaneous follicle center lymphoma (PCFCL) is a rare mature B-cell lymphoma with an unknown etiology.
31042459	1	21	theme	cutaneous	138:146	arg1	lymphoma					164:171	Primary cutaneous follicle center lymphoma	130:171	Primary cutaneous follicle center lymphoma (PCFCL)	130:179	Primary cutaneous follicle center lymphoma (PCFCL) is a rare mature B-cell lymphoma with an unknown etiology.
31042459	1	21	theme	cutaneous	138:146	arg1	lymphoma					205:212	a rare mature B-cell lymphoma	184:212	a rare mature B-cell lymphoma with an unknown etiology	184:237	Primary cutaneous follicle center lymphoma (PCFCL) is a rare mature B-cell lymphoma with an unknown etiology.
31042459	9	22	from	positions	1577:1585	arg1	located					1432:1438	located	1432:1438	located	1432:1438	These motifs were located at the BCR positions converted to N-linked glycosylation motifs in normal B-cell repertoires with low prevalence but mostly at different positions than those found in FL.
31042459	9	23	theme	normal	1507:1512	arg1	repertoires					1521:1531	normal B-cell repertoires	1507:1531	normal B-cell repertoires	1507:1531	These motifs were located at the BCR positions converted to N-linked glycosylation motifs in normal B-cell repertoires with low prevalence but mostly at different positions than those found in FL.
31042459	3	24	theme	FL	333:334	arg1	cells					338:342	FL B cells	333:342	FL B cells	333:342	FL B cells are selected for N-linked glycosylation motifs in their B-cell receptors (BCRs) that are acquired during continuous somatic hypermutation.
31042459	10	25	theme	commensal	1685:1693	arg1	bacteria					1700:1707	commensal skin bacteria	1685:1707	commensal skin bacteria in PCFCL lymphomagenesis	1685:1732	The cutaneous localization of PCFCL might suggest a role for lectins from commensal skin bacteria in PCFCL lymphomagenesis.
31042459	8	26	theme	BCR	1341:1343	arg1	genes					1345:1349	BCR genes	1341:1349	BCR genes of 15 PCFCLs (83%)	1341:1368	Similar to FL but in contrast to primary cutaneous diffuse large B-cell lymphoma, leg-type, BCR genes of 15 PCFCLs (83%) had acquired N-linked glycosylation motifs.
31042459	0	27	theme	Primary	62:68	arg1	Lymphoma					87:94	Primary Cutaneous B-Cell Lymphoma	62:94	Primary Cutaneous B-Cell Lymphoma	62:94	Acquired N-Linked Glycosylation Motifs in B-Cell Receptors of Primary Cutaneous B-Cell Lymphoma and the Normal B-Cell Repertoire.
31042459	1	28	theme	center	157:162	arg1	PCFCL					174:178	PCFCL	174:178	PCFCL	174:178	Primary cutaneous follicle center lymphoma (PCFCL) is a rare mature B-cell lymphoma with an unknown etiology.
31042459	1	28	theme	center	157:162	arg1	lymphoma					164:171	Primary cutaneous follicle center lymphoma	130:171	Primary cutaneous follicle center lymphoma (PCFCL)	130:179	Primary cutaneous follicle center lymphoma (PCFCL) is a rare mature B-cell lymphoma with an unknown etiology.
31042459	1	28	theme	center	157:162	arg1	lymphoma					205:212	a rare mature B-cell lymphoma	184:212	a rare mature B-cell lymphoma with an unknown etiology	184:237	Primary cutaneous follicle center lymphoma (PCFCL) is a rare mature B-cell lymphoma with an unknown etiology.
31042459	8	29	link	N-linked	1383:1390	arg1	motifs					1406:1411	acquired N-linked glycosylation motifs	1374:1411	acquired N-linked glycosylation motifs	1374:1411	Similar to FL but in contrast to primary cutaneous diffuse large B-cell lymphoma, leg-type, BCR genes of 15 PCFCLs (83%) had acquired N-linked glycosylation motifs.
31042459	0	30	theme	B-Cell	80:85	arg1	Lymphoma					87:94	Primary Cutaneous B-Cell Lymphoma	62:94	Primary Cutaneous B-Cell Lymphoma	62:94	Acquired N-Linked Glycosylation Motifs in B-Cell Receptors of Primary Cutaneous B-Cell Lymphoma and the Normal B-Cell Repertoire.
31042459	4	31	theme	BCR	515:517	arg1	stimulation					487:497	The stimulation	483:497	The stimulation of mannosylated BCR by lectins on the tumor microenvironment	483:558	The stimulation of mannosylated BCR by lectins on the tumor microenvironment is therefore a candidate driver in FL pathogenesis.
31042459	4	31	theme	BCR	515:517	arg1	driver					585:590	a candidate driver	573:590	a candidate driver in FL pathogenesis	573:609	The stimulation of mannosylated BCR by lectins on the tumor microenvironment is therefore a candidate driver in FL pathogenesis.
31042459	8	32	theme	PCFCLs	1357:1362	arg1	genes					1345:1349	BCR genes	1341:1349	BCR genes of 15 PCFCLs (83%)	1341:1368	Similar to FL but in contrast to primary cutaneous diffuse large B-cell lymphoma, leg-type, BCR genes of 15 PCFCLs (83%) had acquired N-linked glycosylation motifs.
31042459	9	33	theme	B-cell	1514:1519	arg1	repertoires					1521:1531	normal B-cell repertoires	1507:1531	normal B-cell repertoires	1507:1531	These motifs were located at the BCR positions converted to N-linked glycosylation motifs in normal B-cell repertoires with low prevalence but mostly at different positions than those found in FL.
31042459	9	34	located	found	1598:1602	arg2	those					1592:1596	those	1592:1596	those	1592:1596	These motifs were located at the BCR positions converted to N-linked glycosylation motifs in normal B-cell repertoires with low prevalence but mostly at different positions than those found in FL.
31042459	9	34	located	found	1598:1602	arg1	FL					1607:1608	FL	1607:1608	FL	1607:1608	These motifs were located at the BCR positions converted to N-linked glycosylation motifs in normal B-cell repertoires with low prevalence but mostly at different positions than those found in FL.
31042459	0	35	theme	Normal	104:109	arg1	Repertoire					118:127	the Normal B-Cell Repertoire	100:127	the Normal B-Cell Repertoire	100:127	Acquired N-Linked Glycosylation Motifs in B-Cell Receptors of Primary Cutaneous B-Cell Lymphoma and the Normal B-Cell Repertoire.
31042459	3	36	from	motifs	384:389	arg1	BCRs					418:421	BCRs	418:421	BCRs	418:421	FL B cells are selected for N-linked glycosylation motifs in their B-cell receptors (BCRs) that are acquired during continuous somatic hypermutation.
31042459	3	36	from	motifs	384:389	arg1	receptors					407:415	their B-cell receptors	394:415	their B-cell receptors (BCRs) that are acquired during continuous somatic hypermutation	394:480	FL B cells are selected for N-linked glycosylation motifs in their B-cell receptors (BCRs) that are acquired during continuous somatic hypermutation.
31042459	0	37	theme	B-Cell	42:47	arg1	Receptors					49:57	B-Cell Receptors	42:57	B-Cell Receptors of Primary Cutaneous B-Cell Lymphoma	42:94	Acquired N-Linked Glycosylation Motifs in B-Cell Receptors of Primary Cutaneous B-Cell Lymphoma and the Normal B-Cell Repertoire.
31042459	9	38	theme	different	1567:1575	arg1	positions					1577:1585	different positions	1567:1585	different positions	1567:1585	These motifs were located at the BCR positions converted to N-linked glycosylation motifs in normal B-cell repertoires with low prevalence but mostly at different positions than those found in FL.
31042459	8	39	contain	had	1370:1372	arg1	genes					1345:1349	BCR genes	1341:1349	BCR genes of 15 PCFCLs (83%)	1341:1368	Similar to FL but in contrast to primary cutaneous diffuse large B-cell lymphoma, leg-type, BCR genes of 15 PCFCLs (83%) had acquired N-linked glycosylation motifs.
31042459	8	39	contain	had	1370:1372	arg2	motifs					1406:1411	acquired N-linked glycosylation motifs	1374:1411	acquired N-linked glycosylation motifs	1374:1411	Similar to FL but in contrast to primary cutaneous diffuse large B-cell lymphoma, leg-type, BCR genes of 15 PCFCLs (83%) had acquired N-linked glycosylation motifs.
31042459	6	40	theme	Full-length	696:706	arg1	variable					719:726	Full-length functional variable	696:726	Full-length functional variable	696:726	Full-length functional variable, diversity, and joining gene sequences of 18 PCFCL and 8 primary cutaneous diffuse large B-cell lymphoma, leg-type were identified by unbiased Anchoring Reverse Transcription of Immunoglobulin Sequences and Amplification by Nested PCR and BCR reconstruction from RNA sequencing data.
31042459	3	41	theme	N-linked	361:368	arg1	motifs					384:389	N-linked glycosylation motifs	361:389	N-linked glycosylation motifs in their B-cell receptors (BCRs) that are acquired during continuous somatic hypermutation	361:480	FL B cells are selected for N-linked glycosylation motifs in their B-cell receptors (BCRs) that are acquired during continuous somatic hypermutation.
31042459	8	42	theme	acquired	1374:1381	arg1	motifs					1406:1411	acquired N-linked glycosylation motifs	1374:1411	acquired N-linked glycosylation motifs	1374:1411	Similar to FL but in contrast to primary cutaneous diffuse large B-cell lymphoma, leg-type, BCR genes of 15 PCFCLs (83%) had acquired N-linked glycosylation motifs.
31042459	3	43	theme	continuous	449:458	arg1	hypermutation					468:480	continuous somatic hypermutation	449:480	continuous somatic hypermutation	449:480	FL B cells are selected for N-linked glycosylation motifs in their B-cell receptors (BCRs) that are acquired during continuous somatic hypermutation.
31042459	0	44	theme	Glycosylation	18:30	arg1	Motifs					32:37	N-Linked Glycosylation Motifs	9:37	N-Linked Glycosylation Motifs in B-Cell Receptors of Primary Cutaneous B-Cell Lymphoma	9:94	Acquired N-Linked Glycosylation Motifs in B-Cell Receptors of Primary Cutaneous B-Cell Lymphoma and the Normal B-Cell Repertoire.
31042459	1	45	with	lymphoma	205:212	arg1	etiology					230:237	an unknown etiology	219:237	an unknown etiology	219:237	Primary cutaneous follicle center lymphoma (PCFCL) is a rare mature B-cell lymphoma with an unknown etiology.
31042459	6	46	theme	Anchoring	871:879	arg1	Transcription					889:901	unbiased Anchoring Reverse Transcription	862:901	unbiased Anchoring Reverse Transcription of Immunoglobulin Sequences	862:929	Full-length functional variable, diversity, and joining gene sequences of 18 PCFCL and 8 primary cutaneous diffuse large B-cell lymphoma, leg-type were identified by unbiased Anchoring Reverse Transcription of Immunoglobulin Sequences and Amplification by Nested PCR and BCR reconstruction from RNA sequencing data.
31042459	8	47	theme	glycosylation	1392:1404	arg1	motifs					1406:1411	acquired N-linked glycosylation motifs	1374:1411	acquired N-linked glycosylation motifs	1374:1411	Similar to FL but in contrast to primary cutaneous diffuse large B-cell lymphoma, leg-type, BCR genes of 15 PCFCLs (83%) had acquired N-linked glycosylation motifs.
31042459	10	48	theme	PCFCL	1712:1716	arg1	lymphomagenesis					1718:1732	PCFCL lymphomagenesis	1712:1732	PCFCL lymphomagenesis	1712:1732	The cutaneous localization of PCFCL might suggest a role for lectins from commensal skin bacteria in PCFCL lymphomagenesis.
31042459	0	49	from	Motifs	32:37	arg1	Receptors					49:57	B-Cell Receptors	42:57	B-Cell Receptors of Primary Cutaneous B-Cell Lymphoma	42:94	Acquired N-Linked Glycosylation Motifs in B-Cell Receptors of Primary Cutaneous B-Cell Lymphoma and the Normal B-Cell Repertoire.
31042459	6	50	theme	gene	752:755	arg1	sequences					757:765	joining gene sequences	744:765	joining gene sequences of 18 PCFCL and 8 primary cutaneous diffuse large B-cell lymphoma, leg-type	744:841	Full-length functional variable, diversity, and joining gene sequences of 18 PCFCL and 8 primary cutaneous diffuse large B-cell lymphoma, leg-type were identified by unbiased Anchoring Reverse Transcription of Immunoglobulin Sequences and Amplification by Nested PCR and BCR reconstruction from RNA sequencing data.
31042459	1	51	theme	unknown	222:228	arg1	etiology					230:237	an unknown etiology	219:237	an unknown etiology	219:237	Primary cutaneous follicle center lymphoma (PCFCL) is a rare mature B-cell lymphoma with an unknown etiology.
31042459	0	52	from	Repertoire	118:127	arg1	Receptors					49:57	B-Cell Receptors	42:57	B-Cell Receptors of Primary Cutaneous B-Cell Lymphoma	42:94	Acquired N-Linked Glycosylation Motifs in B-Cell Receptors of Primary Cutaneous B-Cell Lymphoma and the Normal B-Cell Repertoire.
31042459	7	53	theme	large	1123:1127	arg1	lymphoma					1136:1143	primary cutaneous diffuse large B-cell lymphoma	1097:1143	primary cutaneous diffuse large B-cell lymphoma	1097:1143	Low BCR variation demonstrated negligible ongoing somatic hypermutation in PCFCL and primary cutaneous diffuse large B-cell lymphoma, leg-type, and indicated that the PCFCL microarchitecture does not act as a functional germinal center.
31042459	10	54	from	bacteria	1700:1707	arg1	lectins					1672:1678	lectins	1672:1678	lectins from commensal skin bacteria in PCFCL lymphomagenesis	1672:1732	The cutaneous localization of PCFCL might suggest a role for lectins from commensal skin bacteria in PCFCL lymphomagenesis.
31042459	10	54	from	bacteria	1700:1707	arg1	lymphomagenesis					1718:1732	PCFCL lymphomagenesis	1712:1732	PCFCL lymphomagenesis	1712:1732	The cutaneous localization of PCFCL might suggest a role for lectins from commensal skin bacteria in PCFCL lymphomagenesis.
31042459	4	55	theme	candidate	575:583	arg1	stimulation					487:497	The stimulation	483:497	The stimulation of mannosylated BCR by lectins on the tumor microenvironment	483:558	The stimulation of mannosylated BCR by lectins on the tumor microenvironment is therefore a candidate driver in FL pathogenesis.
31042459	4	55	theme	candidate	575:583	arg1	driver					585:590	a candidate driver	573:590	a candidate driver in FL pathogenesis	573:609	The stimulation of mannosylated BCR by lectins on the tumor microenvironment is therefore a candidate driver in FL pathogenesis.
31042459	6	56	theme	large	811:815	arg1	leg-type					834:841	leg-type	834:841	leg-type	834:841	Full-length functional variable, diversity, and joining gene sequences of 18 PCFCL and 8 primary cutaneous diffuse large B-cell lymphoma, leg-type were identified by unbiased Anchoring Reverse Transcription of Immunoglobulin Sequences and Amplification by Nested PCR and BCR reconstruction from RNA sequencing data.
31042459	6	56	theme	large	811:815	arg1	lymphoma					824:831	cutaneous diffuse large B-cell lymphoma	793:831	8 primary cutaneous diffuse large B-cell lymphoma	783:831	Full-length functional variable, diversity, and joining gene sequences of 18 PCFCL and 8 primary cutaneous diffuse large B-cell lymphoma, leg-type were identified by unbiased Anchoring Reverse Transcription of Immunoglobulin Sequences and Amplification by Nested PCR and BCR reconstruction from RNA sequencing data.
31042459	6	57	theme	Immunoglobulin	906:919	arg1	Sequences					921:929	Immunoglobulin Sequences	906:929	Immunoglobulin Sequences	906:929	Full-length functional variable, diversity, and joining gene sequences of 18 PCFCL and 8 primary cutaneous diffuse large B-cell lymphoma, leg-type were identified by unbiased Anchoring Reverse Transcription of Immunoglobulin Sequences and Amplification by Nested PCR and BCR reconstruction from RNA sequencing data.
31042459	4	58	from	stimulation	487:497	arg1	microenvironment					543:558	the tumor microenvironment	533:558	the tumor microenvironment	533:558	The stimulation of mannosylated BCR by lectins on the tumor microenvironment is therefore a candidate driver in FL pathogenesis.
31042459	6	59	theme	cutaneous	793:801	arg1	leg-type					834:841	leg-type	834:841	leg-type	834:841	Full-length functional variable, diversity, and joining gene sequences of 18 PCFCL and 8 primary cutaneous diffuse large B-cell lymphoma, leg-type were identified by unbiased Anchoring Reverse Transcription of Immunoglobulin Sequences and Amplification by Nested PCR and BCR reconstruction from RNA sequencing data.
31042459	6	59	theme	cutaneous	793:801	arg1	lymphoma					824:831	cutaneous diffuse large B-cell lymphoma	793:831	8 primary cutaneous diffuse large B-cell lymphoma	783:831	Full-length functional variable, diversity, and joining gene sequences of 18 PCFCL and 8 primary cutaneous diffuse large B-cell lymphoma, leg-type were identified by unbiased Anchoring Reverse Transcription of Immunoglobulin Sequences and Amplification by Nested PCR and BCR reconstruction from RNA sequencing data.
31042459	4	60	gly	mannosylated	502:513	arg1	BCR					515:517	mannosylated BCR	502:517	mannosylated BCR	502:517	The stimulation of mannosylated BCR by lectins on the tumor microenvironment is therefore a candidate driver in FL pathogenesis.
31042459	8	61	from	Similar	1249:1255	arg1	contrast					1270:1277	contrast	1270:1277	contrast to primary cutaneous diffuse large B-cell lymphoma, leg-type	1270:1338	Similar to FL but in contrast to primary cutaneous diffuse large B-cell lymphoma, leg-type, BCR genes of 15 PCFCLs (83%) had acquired N-linked glycosylation motifs.
31042459	5	62	theme	same	640:643	arg1	mechanism					645:653	the same mechanism	636:653	the same mechanism	636:653	We investigated whether the same mechanism could play a role in PCFCL pathogenesis.
31042459	2	63	theme	microarchitectural	304:321	arg1	criteria					323:330	cytomorphologic and microarchitectural criteria	284:330	cytomorphologic and microarchitectural criteria	284:330	PCFCL resembles follicular lymphoma (FL) by cytomorphologic and microarchitectural criteria.
31042459	9	64	theme	BCR	1447:1449	arg1	positions					1451:1459	the BCR positions	1443:1459	the BCR positions converted to N-linked glycosylation motifs in normal B-cell repertoires with low prevalence	1443:1551	These motifs were located at the BCR positions converted to N-linked glycosylation motifs in normal B-cell repertoires with low prevalence but mostly at different positions than those found in FL.
31042459	6	65	theme	sequencing	995:1004	arg1	data					1006:1009	RNA sequencing data	991:1009	RNA sequencing data	991:1009	Full-length functional variable, diversity, and joining gene sequences of 18 PCFCL and 8 primary cutaneous diffuse large B-cell lymphoma, leg-type were identified by unbiased Anchoring Reverse Transcription of Immunoglobulin Sequences and Amplification by Nested PCR and BCR reconstruction from RNA sequencing data.
31042459	9	66	from	motifs	1497:1502	arg1	repertoires					1521:1531	normal B-cell repertoires	1507:1531	normal B-cell repertoires	1507:1531	These motifs were located at the BCR positions converted to N-linked glycosylation motifs in normal B-cell repertoires with low prevalence but mostly at different positions than those found in FL.
31042459	7	67	theme	cutaneous	1105:1113	arg1	lymphoma					1136:1143	primary cutaneous diffuse large B-cell lymphoma	1097:1143	primary cutaneous diffuse large B-cell lymphoma	1097:1143	Low BCR variation demonstrated negligible ongoing somatic hypermutation in PCFCL and primary cutaneous diffuse large B-cell lymphoma, leg-type, and indicated that the PCFCL microarchitecture does not act as a functional germinal center.
31042459	6	68	theme	PCFCL	773:777	arg1	sequences					757:765	joining gene sequences	744:765	joining gene sequences of 18 PCFCL and 8 primary cutaneous diffuse large B-cell lymphoma, leg-type	744:841	Full-length functional variable, diversity, and joining gene sequences of 18 PCFCL and 8 primary cutaneous diffuse large B-cell lymphoma, leg-type were identified by unbiased Anchoring Reverse Transcription of Immunoglobulin Sequences and Amplification by Nested PCR and BCR reconstruction from RNA sequencing data.
31042459	6	68	theme	PCFCL	773:777	arg1	diversity					729:737	diversity	729:737	diversity	729:737	Full-length functional variable, diversity, and joining gene sequences of 18 PCFCL and 8 primary cutaneous diffuse large B-cell lymphoma, leg-type were identified by unbiased Anchoring Reverse Transcription of Immunoglobulin Sequences and Amplification by Nested PCR and BCR reconstruction from RNA sequencing data.
31042459	6	68	theme	PCFCL	773:777	arg1	variable					719:726	Full-length functional variable	696:726	Full-length functional variable	696:726	Full-length functional variable, diversity, and joining gene sequences of 18 PCFCL and 8 primary cutaneous diffuse large B-cell lymphoma, leg-type were identified by unbiased Anchoring Reverse Transcription of Immunoglobulin Sequences and Amplification by Nested PCR and BCR reconstruction from RNA sequencing data.
31042459	2	69	theme	cytomorphologic	284:298	arg1	criteria					323:330	cytomorphologic and microarchitectural criteria	284:330	cytomorphologic and microarchitectural criteria	284:330	PCFCL resembles follicular lymphoma (FL) by cytomorphologic and microarchitectural criteria.
31042459	1	70	theme	rare	186:189	arg1	lymphoma					164:171	Primary cutaneous follicle center lymphoma	130:171	Primary cutaneous follicle center lymphoma (PCFCL)	130:179	Primary cutaneous follicle center lymphoma (PCFCL) is a rare mature B-cell lymphoma with an unknown etiology.
31042459	1	70	theme	rare	186:189	arg1	lymphoma					205:212	a rare mature B-cell lymphoma	184:212	a rare mature B-cell lymphoma with an unknown etiology	184:237	Primary cutaneous follicle center lymphoma (PCFCL) is a rare mature B-cell lymphoma with an unknown etiology.
31042459	8	71	theme	primary	1282:1288	arg1	leg-type					1331:1338	leg-type	1331:1338	leg-type	1331:1338	Similar to FL but in contrast to primary cutaneous diffuse large B-cell lymphoma, leg-type, BCR genes of 15 PCFCLs (83%) had acquired N-linked glycosylation motifs.
31042459	8	71	theme	primary	1282:1288	arg1	lymphoma					1321:1328	primary cutaneous diffuse large B-cell lymphoma	1282:1328	primary cutaneous diffuse large B-cell lymphoma	1282:1328	Similar to FL but in contrast to primary cutaneous diffuse large B-cell lymphoma, leg-type, BCR genes of 15 PCFCLs (83%) had acquired N-linked glycosylation motifs.
31042459	10	72	theme	PCFCL	1641:1645	arg1	localization					1625:1636	The cutaneous localization	1611:1636	The cutaneous localization of PCFCL	1611:1645	The cutaneous localization of PCFCL might suggest a role for lectins from commensal skin bacteria in PCFCL lymphomagenesis.
31042459	1	73	theme	B-cell	198:203	arg1	lymphoma					164:171	Primary cutaneous follicle center lymphoma	130:171	Primary cutaneous follicle center lymphoma (PCFCL)	130:179	Primary cutaneous follicle center lymphoma (PCFCL) is a rare mature B-cell lymphoma with an unknown etiology.
31042459	1	73	theme	B-cell	198:203	arg1	lymphoma					205:212	a rare mature B-cell lymphoma	184:212	a rare mature B-cell lymphoma with an unknown etiology	184:237	Primary cutaneous follicle center lymphoma (PCFCL) is a rare mature B-cell lymphoma with an unknown etiology.
31042459	6	74	theme	BCR	967:969	arg1	reconstruction					971:984	BCR reconstruction	967:984	BCR reconstruction	967:984	Full-length functional variable, diversity, and joining gene sequences of 18 PCFCL and 8 primary cutaneous diffuse large B-cell lymphoma, leg-type were identified by unbiased Anchoring Reverse Transcription of Immunoglobulin Sequences and Amplification by Nested PCR and BCR reconstruction from RNA sequencing data.
31042459	8	75	theme	diffuse	1300:1306	arg1	leg-type					1331:1338	leg-type	1331:1338	leg-type	1331:1338	Similar to FL but in contrast to primary cutaneous diffuse large B-cell lymphoma, leg-type, BCR genes of 15 PCFCLs (83%) had acquired N-linked glycosylation motifs.
31042459	8	75	theme	diffuse	1300:1306	arg1	lymphoma					1321:1328	primary cutaneous diffuse large B-cell lymphoma	1282:1328	primary cutaneous diffuse large B-cell lymphoma	1282:1328	Similar to FL but in contrast to primary cutaneous diffuse large B-cell lymphoma, leg-type, BCR genes of 15 PCFCLs (83%) had acquired N-linked glycosylation motifs.
31042459	7	76	theme	ongoing	1054:1060	arg1	hypermutation					1070:1082	negligible ongoing somatic hypermutation	1043:1082	negligible ongoing somatic hypermutation	1043:1082	Low BCR variation demonstrated negligible ongoing somatic hypermutation in PCFCL and primary cutaneous diffuse large B-cell lymphoma, leg-type, and indicated that the PCFCL microarchitecture does not act as a functional germinal center.
31042459	8	77	theme	B-cell	1314:1319	arg1	leg-type					1331:1338	leg-type	1331:1338	leg-type	1331:1338	Similar to FL but in contrast to primary cutaneous diffuse large B-cell lymphoma, leg-type, BCR genes of 15 PCFCLs (83%) had acquired N-linked glycosylation motifs.
31042459	8	77	theme	B-cell	1314:1319	arg1	lymphoma					1321:1328	primary cutaneous diffuse large B-cell lymphoma	1282:1328	primary cutaneous diffuse large B-cell lymphoma	1282:1328	Similar to FL but in contrast to primary cutaneous diffuse large B-cell lymphoma, leg-type, BCR genes of 15 PCFCLs (83%) had acquired N-linked glycosylation motifs.
31042459	7	78	theme	somatic	1062:1068	arg1	hypermutation					1070:1082	negligible ongoing somatic hypermutation	1043:1082	negligible ongoing somatic hypermutation	1043:1082	Low BCR variation demonstrated negligible ongoing somatic hypermutation in PCFCL and primary cutaneous diffuse large B-cell lymphoma, leg-type, and indicated that the PCFCL microarchitecture does not act as a functional germinal center.
31042459	3	79	link	N-linked	361:368	arg1	motifs					384:389	N-linked glycosylation motifs	361:389	N-linked glycosylation motifs in their B-cell receptors (BCRs) that are acquired during continuous somatic hypermutation	361:480	FL B cells are selected for N-linked glycosylation motifs in their B-cell receptors (BCRs) that are acquired during continuous somatic hypermutation.
31042459	1	80	theme	Primary	130:136	arg1	PCFCL					174:178	PCFCL	174:178	PCFCL	174:178	Primary cutaneous follicle center lymphoma (PCFCL) is a rare mature B-cell lymphoma with an unknown etiology.
31042459	1	80	theme	Primary	130:136	arg1	lymphoma					164:171	Primary cutaneous follicle center lymphoma	130:171	Primary cutaneous follicle center lymphoma (PCFCL)	130:179	Primary cutaneous follicle center lymphoma (PCFCL) is a rare mature B-cell lymphoma with an unknown etiology.
31042459	1	80	theme	Primary	130:136	arg1	lymphoma					205:212	a rare mature B-cell lymphoma	184:212	a rare mature B-cell lymphoma with an unknown etiology	184:237	Primary cutaneous follicle center lymphoma (PCFCL) is a rare mature B-cell lymphoma with an unknown etiology.
31042459	9	81	gly	glycosylation	1483:1495	arg2	motifs					1497:1502	N-linked glycosylation motifs	1474:1502	N-linked glycosylation motifs in normal B-cell repertoires	1474:1531	These motifs were located at the BCR positions converted to N-linked glycosylation motifs in normal B-cell repertoires with low prevalence but mostly at different positions than those found in FL.
31042459	0	82	theme	Cutaneous	70:78	arg1	Lymphoma					87:94	Primary Cutaneous B-Cell Lymphoma	62:94	Primary Cutaneous B-Cell Lymphoma	62:94	Acquired N-Linked Glycosylation Motifs in B-Cell Receptors of Primary Cutaneous B-Cell Lymphoma and the Normal B-Cell Repertoire.
31042459	7	83	theme	BCR	1016:1018	arg1	variation					1020:1028	Low BCR variation	1012:1028	Low BCR variation	1012:1028	Low BCR variation demonstrated negligible ongoing somatic hypermutation in PCFCL and primary cutaneous diffuse large B-cell lymphoma, leg-type, and indicated that the PCFCL microarchitecture does not act as a functional germinal center.
31042459	1	84	theme	follicle	148:155	arg1	PCFCL					174:178	PCFCL	174:178	PCFCL	174:178	Primary cutaneous follicle center lymphoma (PCFCL) is a rare mature B-cell lymphoma with an unknown etiology.
31042459	1	84	theme	follicle	148:155	arg1	lymphoma					164:171	Primary cutaneous follicle center lymphoma	130:171	Primary cutaneous follicle center lymphoma (PCFCL)	130:179	Primary cutaneous follicle center lymphoma (PCFCL) is a rare mature B-cell lymphoma with an unknown etiology.
31042459	1	84	theme	follicle	148:155	arg1	lymphoma					205:212	a rare mature B-cell lymphoma	184:212	a rare mature B-cell lymphoma with an unknown etiology	184:237	Primary cutaneous follicle center lymphoma (PCFCL) is a rare mature B-cell lymphoma with an unknown etiology.
31042459	3	85	theme	B	336:336	arg1	cells					338:342	FL B cells	333:342	FL B cells	333:342	FL B cells are selected for N-linked glycosylation motifs in their B-cell receptors (BCRs) that are acquired during continuous somatic hypermutation.
31042459	4	86	theme	mannosylated	502:513	arg1	BCR					515:517	mannosylated BCR	502:517	mannosylated BCR	502:517	The stimulation of mannosylated BCR by lectins on the tumor microenvironment is therefore a candidate driver in FL pathogenesis.
31042459	0	87	theme	Lymphoma	87:94	arg1	Receptors					49:57	B-Cell Receptors	42:57	B-Cell Receptors of Primary Cutaneous B-Cell Lymphoma	42:94	Acquired N-Linked Glycosylation Motifs in B-Cell Receptors of Primary Cutaneous B-Cell Lymphoma and the Normal B-Cell Repertoire.
31042459	9	88	link	N-linked	1474:1481	arg1	motifs					1497:1502	N-linked glycosylation motifs	1474:1502	N-linked glycosylation motifs in normal B-cell repertoires	1474:1531	These motifs were located at the BCR positions converted to N-linked glycosylation motifs in normal B-cell repertoires with low prevalence but mostly at different positions than those found in FL.
31042459	9	89	theme	low	1538:1540	arg1	prevalence					1542:1551	low prevalence	1538:1551	low prevalence	1538:1551	These motifs were located at the BCR positions converted to N-linked glycosylation motifs in normal B-cell repertoires with low prevalence but mostly at different positions than those found in FL.
31042459	7	90	theme	germinal	1232:1239	arg1	microarchitecture					1185:1201	the PCFCL microarchitecture	1175:1201	the PCFCL microarchitecture	1175:1201	Low BCR variation demonstrated negligible ongoing somatic hypermutation in PCFCL and primary cutaneous diffuse large B-cell lymphoma, leg-type, and indicated that the PCFCL microarchitecture does not act as a functional germinal center.
31042459	7	90	theme	germinal	1232:1239	arg1	center					1241:1246	a functional germinal center	1219:1246	a functional germinal center	1219:1246	Low BCR variation demonstrated negligible ongoing somatic hypermutation in PCFCL and primary cutaneous diffuse large B-cell lymphoma, leg-type, and indicated that the PCFCL microarchitecture does not act as a functional germinal center.
31042459	0	91	theme	B-Cell	111:116	arg1	Repertoire					118:127	the Normal B-Cell Repertoire	100:127	the Normal B-Cell Repertoire	100:127	Acquired N-Linked Glycosylation Motifs in B-Cell Receptors of Primary Cutaneous B-Cell Lymphoma and the Normal B-Cell Repertoire.
31042459	6	92	from	data	1006:1009	arg1	PCR					959:961	Nested PCR	952:961	Nested PCR	952:961	Full-length functional variable, diversity, and joining gene sequences of 18 PCFCL and 8 primary cutaneous diffuse large B-cell lymphoma, leg-type were identified by unbiased Anchoring Reverse Transcription of Immunoglobulin Sequences and Amplification by Nested PCR and BCR reconstruction from RNA sequencing data.
31042459	6	92	from	data	1006:1009	arg1	reconstruction					971:984	BCR reconstruction	967:984	BCR reconstruction	967:984	Full-length functional variable, diversity, and joining gene sequences of 18 PCFCL and 8 primary cutaneous diffuse large B-cell lymphoma, leg-type were identified by unbiased Anchoring Reverse Transcription of Immunoglobulin Sequences and Amplification by Nested PCR and BCR reconstruction from RNA sequencing data.
31042459	6	93	theme	functional	708:717	arg1	variable					719:726	Full-length functional variable	696:726	Full-length functional variable	696:726	Full-length functional variable, diversity, and joining gene sequences of 18 PCFCL and 8 primary cutaneous diffuse large B-cell lymphoma, leg-type were identified by unbiased Anchoring Reverse Transcription of Immunoglobulin Sequences and Amplification by Nested PCR and BCR reconstruction from RNA sequencing data.
31042459	4	94	from	driver	585:590	arg1	pathogenesis					598:609	FL pathogenesis	595:609	FL pathogenesis	595:609	The stimulation of mannosylated BCR by lectins on the tumor microenvironment is therefore a candidate driver in FL pathogenesis.
31042459	0	95	theme	N-Linked	9:16	arg1	Motifs					32:37	N-Linked Glycosylation Motifs	9:37	N-Linked Glycosylation Motifs in B-Cell Receptors of Primary Cutaneous B-Cell Lymphoma	9:94	Acquired N-Linked Glycosylation Motifs in B-Cell Receptors of Primary Cutaneous B-Cell Lymphoma and the Normal B-Cell Repertoire.
31042459	3	96	gly	glycosylation	370:382	arg2	motifs					384:389	N-linked glycosylation motifs	361:389	N-linked glycosylation motifs in their B-cell receptors (BCRs) that are acquired during continuous somatic hypermutation	361:480	FL B cells are selected for N-linked glycosylation motifs in their B-cell receptors (BCRs) that are acquired during continuous somatic hypermutation.
31042459	9	97	located	located	1432:1438	arg2	motifs					1420:1425	These motifs	1414:1425	These motifs	1414:1425	These motifs were located at the BCR positions converted to N-linked glycosylation motifs in normal B-cell repertoires with low prevalence but mostly at different positions than those found in FL.
31042459	9	97	located	located	1432:1438	arg1	positions					1451:1459	the BCR positions	1443:1459	the BCR positions converted to N-linked glycosylation motifs in normal B-cell repertoires with low prevalence	1443:1551	These motifs were located at the BCR positions converted to N-linked glycosylation motifs in normal B-cell repertoires with low prevalence but mostly at different positions than those found in FL.
31042459	3	98	theme	glycosylation	370:382	arg1	motifs					384:389	N-linked glycosylation motifs	361:389	N-linked glycosylation motifs in their B-cell receptors (BCRs) that are acquired during continuous somatic hypermutation	361:480	FL B cells are selected for N-linked glycosylation motifs in their B-cell receptors (BCRs) that are acquired during continuous somatic hypermutation.
31042459	3	99	theme	somatic	460:466	arg1	hypermutation					468:480	continuous somatic hypermutation	449:480	continuous somatic hypermutation	449:480	FL B cells are selected for N-linked glycosylation motifs in their B-cell receptors (BCRs) that are acquired during continuous somatic hypermutation.
31042459	7	100	theme	PCFCL	1179:1183	arg1	center					1241:1246	a functional germinal center	1219:1246	a functional germinal center	1219:1246	Low BCR variation demonstrated negligible ongoing somatic hypermutation in PCFCL and primary cutaneous diffuse large B-cell lymphoma, leg-type, and indicated that the PCFCL microarchitecture does not act as a functional germinal center.
31042459	7	100	theme	PCFCL	1179:1183	arg1	microarchitecture					1185:1201	the PCFCL microarchitecture	1175:1201	the PCFCL microarchitecture	1175:1201	Low BCR variation demonstrated negligible ongoing somatic hypermutation in PCFCL and primary cutaneous diffuse large B-cell lymphoma, leg-type, and indicated that the PCFCL microarchitecture does not act as a functional germinal center.
31042459	6	101	theme	unbiased	862:869	arg1	Transcription					889:901	unbiased Anchoring Reverse Transcription	862:901	unbiased Anchoring Reverse Transcription of Immunoglobulin Sequences	862:929	Full-length functional variable, diversity, and joining gene sequences of 18 PCFCL and 8 primary cutaneous diffuse large B-cell lymphoma, leg-type were identified by unbiased Anchoring Reverse Transcription of Immunoglobulin Sequences and Amplification by Nested PCR and BCR reconstruction from RNA sequencing data.
31042459	8	102	theme	N-linked	1383:1390	arg1	motifs					1406:1411	acquired N-linked glycosylation motifs	1374:1411	acquired N-linked glycosylation motifs	1374:1411	Similar to FL but in contrast to primary cutaneous diffuse large B-cell lymphoma, leg-type, BCR genes of 15 PCFCLs (83%) had acquired N-linked glycosylation motifs.
31042459	7	103	theme	functional	1221:1230	arg1	microarchitecture					1185:1201	the PCFCL microarchitecture	1175:1201	the PCFCL microarchitecture	1175:1201	Low BCR variation demonstrated negligible ongoing somatic hypermutation in PCFCL and primary cutaneous diffuse large B-cell lymphoma, leg-type, and indicated that the PCFCL microarchitecture does not act as a functional germinal center.
31042459	7	103	theme	functional	1221:1230	arg1	center					1241:1246	a functional germinal center	1219:1246	a functional germinal center	1219:1246	Low BCR variation demonstrated negligible ongoing somatic hypermutation in PCFCL and primary cutaneous diffuse large B-cell lymphoma, leg-type, and indicated that the PCFCL microarchitecture does not act as a functional germinal center.
31042459	9	104	theme	N-linked	1474:1481	arg1	motifs					1497:1502	N-linked glycosylation motifs	1474:1502	N-linked glycosylation motifs in normal B-cell repertoires	1474:1531	These motifs were located at the BCR positions converted to N-linked glycosylation motifs in normal B-cell repertoires with low prevalence but mostly at different positions than those found in FL.
31042459	3	105	theme	B-cell	400:405	arg1	BCRs					418:421	BCRs	418:421	BCRs	418:421	FL B cells are selected for N-linked glycosylation motifs in their B-cell receptors (BCRs) that are acquired during continuous somatic hypermutation.
31042459	3	105	theme	B-cell	400:405	arg1	receptors					407:415	their B-cell receptors	394:415	their B-cell receptors (BCRs) that are acquired during continuous somatic hypermutation	394:480	FL B cells are selected for N-linked glycosylation motifs in their B-cell receptors (BCRs) that are acquired during continuous somatic hypermutation.
31042459	4	106	theme	tumor	537:541	arg1	microenvironment					543:558	the tumor microenvironment	533:558	the tumor microenvironment	533:558	The stimulation of mannosylated BCR by lectins on the tumor microenvironment is therefore a candidate driver in FL pathogenesis.
31042459	6	107	theme	joining	744:750	arg1	sequences					757:765	joining gene sequences	744:765	joining gene sequences of 18 PCFCL and 8 primary cutaneous diffuse large B-cell lymphoma, leg-type	744:841	Full-length functional variable, diversity, and joining gene sequences of 18 PCFCL and 8 primary cutaneous diffuse large B-cell lymphoma, leg-type were identified by unbiased Anchoring Reverse Transcription of Immunoglobulin Sequences and Amplification by Nested PCR and BCR reconstruction from RNA sequencing data.
31042459	10	108	theme	skin	1695:1698	arg1	bacteria					1700:1707	commensal skin bacteria	1685:1707	commensal skin bacteria in PCFCL lymphomagenesis	1685:1732	The cutaneous localization of PCFCL might suggest a role for lectins from commensal skin bacteria in PCFCL lymphomagenesis.
31042459	8	109	gly	glycosylation	1392:1404	arg2	motifs					1406:1411	acquired N-linked glycosylation motifs	1374:1411	acquired N-linked glycosylation motifs	1374:1411	Similar to FL but in contrast to primary cutaneous diffuse large B-cell lymphoma, leg-type, BCR genes of 15 PCFCLs (83%) had acquired N-linked glycosylation motifs.
31042459	6	110	theme	lymphoma	824:831	arg1	sequences					757:765	joining gene sequences	744:765	joining gene sequences of 18 PCFCL and 8 primary cutaneous diffuse large B-cell lymphoma, leg-type	744:841	Full-length functional variable, diversity, and joining gene sequences of 18 PCFCL and 8 primary cutaneous diffuse large B-cell lymphoma, leg-type were identified by unbiased Anchoring Reverse Transcription of Immunoglobulin Sequences and Amplification by Nested PCR and BCR reconstruction from RNA sequencing data.
31042459	6	110	theme	lymphoma	824:831	arg1	diversity					729:737	diversity	729:737	diversity	729:737	Full-length functional variable, diversity, and joining gene sequences of 18 PCFCL and 8 primary cutaneous diffuse large B-cell lymphoma, leg-type were identified by unbiased Anchoring Reverse Transcription of Immunoglobulin Sequences and Amplification by Nested PCR and BCR reconstruction from RNA sequencing data.
31042459	6	110	theme	lymphoma	824:831	arg1	variable					719:726	Full-length functional variable	696:726	Full-length functional variable	696:726	Full-length functional variable, diversity, and joining gene sequences of 18 PCFCL and 8 primary cutaneous diffuse large B-cell lymphoma, leg-type were identified by unbiased Anchoring Reverse Transcription of Immunoglobulin Sequences and Amplification by Nested PCR and BCR reconstruction from RNA sequencing data.
31042459	9	111	from	positions	1451:1459	arg1	located					1432:1438	located	1432:1438	located	1432:1438	These motifs were located at the BCR positions converted to N-linked glycosylation motifs in normal B-cell repertoires with low prevalence but mostly at different positions than those found in FL.
31002798	0	0	theme	Gene	83:86	arg1	Expression					88:97	Proteasome Gene Expression	72:97	Proteasome Gene Expression	72:97	Protein Sequence Editing of SKN-1A/Nrf1 by Peptide:N-Glycanase Controls Proteasome Gene Expression.
31002798	2	1	gly	glycosylation	321:333	arg1	factor					290:295	an endoplasmic reticulum (ER)-associated transcription factor	235:295	an endoplasmic reticulum (ER)-associated transcription factor that undergoes N-linked glycosylation	235:333	SKN-1A/Nrf1, an endoplasmic reticulum (ER)-associated transcription factor that undergoes N-linked glycosylation, serves as a sensor of proteasome dysfunction and triggers compensatory upregulation of proteasome subunit genes.
31002798	2	1	gly	glycosylation	321:333	arg1	SKN-1A/Nrf1					222:232	SKN-1A/Nrf1	222:232	SKN-1A/Nrf1	222:232	SKN-1A/Nrf1, an endoplasmic reticulum (ER)-associated transcription factor that undergoes N-linked glycosylation, serves as a sensor of proteasome dysfunction and triggers compensatory upregulation of proteasome subunit genes.
31002798	3	2	gly	N-glycosylated	566:579	arg1	residues					592:599	particular N-glycosylated asparagine residues	555:599	particular N-glycosylated asparagine residues	555:599	Here, we show that the PNG-1/NGLY1 peptide:N-glycanase edits the sequence of SKN-1A protein by converting particular N-glycosylated asparagine residues to aspartic acid.
31002798	6	3	theme	cytoprotective	1130:1143	arg1	functions					1145:1153	distinct cytoprotective functions	1121:1153	distinct cytoprotective functions corresponding to those of mammalian Nrf1 and Nrf2	1121:1203	Deglycosylation-dependent protein sequence editing explains how ER-associated and cytosolic isoforms of SKN-1 perform distinct cytoprotective functions corresponding to those of mammalian Nrf1 and Nrf2.
31002798	0	4	theme	Proteasome	72:81	arg1	Expression					88:97	Proteasome Gene Expression	72:97	Proteasome Gene Expression	72:97	Protein Sequence Editing of SKN-1A/Nrf1 by Peptide:N-Glycanase Controls Proteasome Gene Expression.
31002798	6	5	theme	Deglycosylation-dependent	1003:1027	arg1	editing					1046:1052	Deglycosylation-dependent protein sequence editing	1003:1052	Deglycosylation-dependent protein sequence editing	1003:1052	Deglycosylation-dependent protein sequence editing explains how ER-associated and cytosolic isoforms of SKN-1 perform distinct cytoprotective functions corresponding to those of mammalian Nrf1 and Nrf2.
31002798	7	6	theme	N-linked	1256:1263	arg1	glycosylation					1265:1277	N-linked glycosylation	1256:1277	N-linked glycosylation	1256:1277	Thus, we uncover an unexpected mechanism by which N-linked glycosylation regulates protein function and proteostasis.
31002798	5	7	theme	beta	989:992	arg1	peptide					994:1000	human amyloid beta peptide	975:1000	human amyloid beta peptide	975:1000	This pathway is required to maintain sufficient proteasome expression and activity, and SKN-1A hyperactivation confers resistance to the proteotoxicity of human amyloid beta peptide.
31002798	2	8	theme	dysfunction	369:379	arg1	upregulation					407:418	compensatory upregulation	394:418	compensatory upregulation of proteasome subunit genes	394:446	SKN-1A/Nrf1, an endoplasmic reticulum (ER)-associated transcription factor that undergoes N-linked glycosylation, serves as a sensor of proteasome dysfunction and triggers compensatory upregulation of proteasome subunit genes.
31002798	2	8	theme	dysfunction	369:379	arg1	SKN-1A/Nrf1					222:232	SKN-1A/Nrf1	222:232	SKN-1A/Nrf1	222:232	SKN-1A/Nrf1, an endoplasmic reticulum (ER)-associated transcription factor that undergoes N-linked glycosylation, serves as a sensor of proteasome dysfunction and triggers compensatory upregulation of proteasome subunit genes.
31002798	2	8	theme	dysfunction	369:379	arg1	sensor					348:353	a sensor	346:353	a sensor of proteasome dysfunction	346:379	SKN-1A/Nrf1, an endoplasmic reticulum (ER)-associated transcription factor that undergoes N-linked glycosylation, serves as a sensor of proteasome dysfunction and triggers compensatory upregulation of proteasome subunit genes.
31002798	6	9	theme	distinct	1121:1128	arg1	functions					1145:1153	distinct cytoprotective functions	1121:1153	distinct cytoprotective functions corresponding to those of mammalian Nrf1 and Nrf2	1121:1203	Deglycosylation-dependent protein sequence editing explains how ER-associated and cytosolic isoforms of SKN-1 perform distinct cytoprotective functions corresponding to those of mammalian Nrf1 and Nrf2.
31002798	3	10	dep	peptide	484:490	arg1	N-glycanase					492:502	N-glycanase	492:502	the PNG-1/NGLY1 peptide:N-glycanase	468:502	Here, we show that the PNG-1/NGLY1 peptide:N-glycanase edits the sequence of SKN-1A protein by converting particular N-glycosylated asparagine residues to aspartic acid.
31002798	4	11	gly	N-glycosylation	663:677	arg2	sites					679:683	these N-glycosylation sites	657:683	these N-glycosylation sites	657:683	Genetically introducing aspartates at these N-glycosylation sites bypasses the requirement for PNG-1/NGLY1, showing that protein sequence editing rather than deglycosylation is key to SKN-1A function.
31002798	2	12	theme	N-linked	312:319	arg1	glycosylation					321:333	N-linked glycosylation	312:333	N-linked glycosylation	312:333	SKN-1A/Nrf1, an endoplasmic reticulum (ER)-associated transcription factor that undergoes N-linked glycosylation, serves as a sensor of proteasome dysfunction and triggers compensatory upregulation of proteasome subunit genes.
31002798	3	13	theme	aspartic	604:611	arg1	acid					613:616	aspartic acid	604:616	aspartic acid	604:616	Here, we show that the PNG-1/NGLY1 peptide:N-glycanase edits the sequence of SKN-1A protein by converting particular N-glycosylated asparagine residues to aspartic acid.
31002798	3	14	theme	SKN-1A	526:531	arg1	protein					533:539	SKN-1A protein	526:539	SKN-1A protein	526:539	Here, we show that the PNG-1/NGLY1 peptide:N-glycanase edits the sequence of SKN-1A protein by converting particular N-glycosylated asparagine residues to aspartic acid.
31002798	6	15	theme	SKN-1	1107:1111	arg1	isoforms					1095:1102	ER-associated and cytosolic isoforms	1067:1102	ER-associated and cytosolic isoforms of SKN-1	1067:1111	Deglycosylation-dependent protein sequence editing explains how ER-associated and cytosolic isoforms of SKN-1 perform distinct cytoprotective functions corresponding to those of mammalian Nrf1 and Nrf2.
31002798	3	16	theme	particular	555:564	arg1	residues					592:599	particular N-glycosylated asparagine residues	555:599	particular N-glycosylated asparagine residues	555:599	Here, we show that the PNG-1/NGLY1 peptide:N-glycanase edits the sequence of SKN-1A protein by converting particular N-glycosylated asparagine residues to aspartic acid.
31002798	2	17	theme	subunit	434:440	arg1	genes					442:446	proteasome subunit genes	423:446	proteasome subunit genes	423:446	SKN-1A/Nrf1, an endoplasmic reticulum (ER)-associated transcription factor that undergoes N-linked glycosylation, serves as a sensor of proteasome dysfunction and triggers compensatory upregulation of proteasome subunit genes.
31002798	5	18	theme	peptide	994:1000	arg1	proteotoxicity					957:970	the proteotoxicity	953:970	the proteotoxicity of human amyloid beta peptide	953:1000	This pathway is required to maintain sufficient proteasome expression and activity, and SKN-1A hyperactivation confers resistance to the proteotoxicity of human amyloid beta peptide.
31002798	7	19	theme	unexpected	1226:1235	arg1	mechanism					1237:1245	an unexpected mechanism	1223:1245	an unexpected mechanism by which N-linked glycosylation regulates protein function and proteostasis	1223:1321	Thus, we uncover an unexpected mechanism by which N-linked glycosylation regulates protein function and proteostasis.
31002798	3	20	theme	N-glycosylated	566:579	arg1	residues					592:599	particular N-glycosylated asparagine residues	555:599	particular N-glycosylated asparagine residues	555:599	Here, we show that the PNG-1/NGLY1 peptide:N-glycanase edits the sequence of SKN-1A protein by converting particular N-glycosylated asparagine residues to aspartic acid.
31002798	2	21	theme	proteasome	423:432	arg1	genes					442:446	proteasome subunit genes	423:446	proteasome subunit genes	423:446	SKN-1A/Nrf1, an endoplasmic reticulum (ER)-associated transcription factor that undergoes N-linked glycosylation, serves as a sensor of proteasome dysfunction and triggers compensatory upregulation of proteasome subunit genes.
31002798	0	22	theme	Sequence	8:15	arg1	Editing					17:23	Protein Sequence Editing	0:23	Protein Sequence Editing of SKN-1A/Nrf1 by Peptide:N-Glycanase	0:61	Protein Sequence Editing of SKN-1A/Nrf1 by Peptide:N-Glycanase Controls Proteasome Gene Expression.
31002798	4	23	theme	protein	740:746	arg1	editing					757:763	protein sequence editing	740:763	protein sequence editing rather than deglycosylation	740:791	Genetically introducing aspartates at these N-glycosylation sites bypasses the requirement for PNG-1/NGLY1, showing that protein sequence editing rather than deglycosylation is key to SKN-1A function.
31002798	0	24	theme	Protein	0:6	arg1	Editing					17:23	Protein Sequence Editing	0:23	Protein Sequence Editing of SKN-1A/Nrf1 by Peptide:N-Glycanase	0:61	Protein Sequence Editing of SKN-1A/Nrf1 by Peptide:N-Glycanase Controls Proteasome Gene Expression.
31002798	4	25	theme	SKN-1A	803:808	arg1	function					810:817	SKN-1A function	803:817	SKN-1A function	803:817	Genetically introducing aspartates at these N-glycosylation sites bypasses the requirement for PNG-1/NGLY1, showing that protein sequence editing rather than deglycosylation is key to SKN-1A function.
31002798	1	26	theme	proteotoxic	198:208	arg1	challenges					210:219	proteotoxic challenges	198:219	proteotoxic challenges	198:219	The proteasome mediates selective protein degradation and is dynamically regulated in response to proteotoxic challenges.
31002798	6	27	theme	cytosolic	1085:1093	arg1	isoforms					1095:1102	ER-associated and cytosolic isoforms	1067:1102	ER-associated and cytosolic isoforms of SKN-1	1067:1111	Deglycosylation-dependent protein sequence editing explains how ER-associated and cytosolic isoforms of SKN-1 perform distinct cytoprotective functions corresponding to those of mammalian Nrf1 and Nrf2.
31002798	5	28	theme	SKN-1A	908:913	arg1	hyperactivation					915:929	SKN-1A hyperactivation	908:929	SKN-1A hyperactivation	908:929	This pathway is required to maintain sufficient proteasome expression and activity, and SKN-1A hyperactivation confers resistance to the proteotoxicity of human amyloid beta peptide.
31002798	2	29	link	N-linked	312:319	arg1	glycosylation					321:333	N-linked glycosylation	312:333	N-linked glycosylation	312:333	SKN-1A/Nrf1, an endoplasmic reticulum (ER)-associated transcription factor that undergoes N-linked glycosylation, serves as a sensor of proteasome dysfunction and triggers compensatory upregulation of proteasome subunit genes.
31002798	3	30	theme	protein	533:539	arg1	sequence					514:521	the sequence	510:521	the sequence of SKN-1A protein	510:539	Here, we show that the PNG-1/NGLY1 peptide:N-glycanase edits the sequence of SKN-1A protein by converting particular N-glycosylated asparagine residues to aspartic acid.
31002798	6	31	theme	protein	1029:1035	arg1	editing					1046:1052	Deglycosylation-dependent protein sequence editing	1003:1052	Deglycosylation-dependent protein sequence editing	1003:1052	Deglycosylation-dependent protein sequence editing explains how ER-associated and cytosolic isoforms of SKN-1 perform distinct cytoprotective functions corresponding to those of mammalian Nrf1 and Nrf2.
31002798	5	32	theme	human	975:979	arg1	peptide					994:1000	human amyloid beta peptide	975:1000	human amyloid beta peptide	975:1000	This pathway is required to maintain sufficient proteasome expression and activity, and SKN-1A hyperactivation confers resistance to the proteotoxicity of human amyloid beta peptide.
31002798	7	33	theme	protein	1289:1295	arg1	function					1297:1304	protein function	1289:1304	protein function	1289:1304	Thus, we uncover an unexpected mechanism by which N-linked glycosylation regulates protein function and proteostasis.
31002798	6	34	theme	mammalian	1181:1189	arg1	Nrf1					1191:1194	Nrf1	1191:1194	Nrf1	1191:1194	Deglycosylation-dependent protein sequence editing explains how ER-associated and cytosolic isoforms of SKN-1 perform distinct cytoprotective functions corresponding to those of mammalian Nrf1 and Nrf2.
31002798	3	35	theme	PNG-1/NGLY1	472:482	arg1	peptide					484:490	the PNG-1/NGLY1 peptide	468:490	the PNG-1/NGLY1 peptide:N-glycanase	468:502	Here, we show that the PNG-1/NGLY1 peptide:N-glycanase edits the sequence of SKN-1A protein by converting particular N-glycosylated asparagine residues to aspartic acid.
31002798	0	36	theme	SKN-1A/Nrf1	28:38	arg1	Editing					17:23	Protein Sequence Editing	0:23	Protein Sequence Editing of SKN-1A/Nrf1 by Peptide:N-Glycanase	0:61	Protein Sequence Editing of SKN-1A/Nrf1 by Peptide:N-Glycanase Controls Proteasome Gene Expression.
31002798	6	37	theme	ER-associated	1067:1079	arg1	isoforms					1095:1102	ER-associated and cytosolic isoforms	1067:1102	ER-associated and cytosolic isoforms of SKN-1	1067:1111	Deglycosylation-dependent protein sequence editing explains how ER-associated and cytosolic isoforms of SKN-1 perform distinct cytoprotective functions corresponding to those of mammalian Nrf1 and Nrf2.
31002798	5	38	theme	sufficient	857:866	arg1	expression					879:888	sufficient proteasome expression	857:888	sufficient proteasome expression	857:888	This pathway is required to maintain sufficient proteasome expression and activity, and SKN-1A hyperactivation confers resistance to the proteotoxicity of human amyloid beta peptide.
31002798	2	39	theme	genes	442:446	arg1	upregulation					407:418	compensatory upregulation	394:418	compensatory upregulation of proteasome subunit genes	394:446	SKN-1A/Nrf1, an endoplasmic reticulum (ER)-associated transcription factor that undergoes N-linked glycosylation, serves as a sensor of proteasome dysfunction and triggers compensatory upregulation of proteasome subunit genes.
31002798	2	39	theme	genes	442:446	arg1	SKN-1A/Nrf1					222:232	SKN-1A/Nrf1	222:232	SKN-1A/Nrf1	222:232	SKN-1A/Nrf1, an endoplasmic reticulum (ER)-associated transcription factor that undergoes N-linked glycosylation, serves as a sensor of proteasome dysfunction and triggers compensatory upregulation of proteasome subunit genes.
31002798	2	39	theme	genes	442:446	arg1	sensor					348:353	a sensor	346:353	a sensor of proteasome dysfunction	346:379	SKN-1A/Nrf1, an endoplasmic reticulum (ER)-associated transcription factor that undergoes N-linked glycosylation, serves as a sensor of proteasome dysfunction and triggers compensatory upregulation of proteasome subunit genes.
31002798	5	40	theme	proteasome	868:877	arg1	expression					879:888	sufficient proteasome expression	857:888	sufficient proteasome expression	857:888	This pathway is required to maintain sufficient proteasome expression and activity, and SKN-1A hyperactivation confers resistance to the proteotoxicity of human amyloid beta peptide.
31002798	2	41	theme	endoplasmic	238:248	arg1	reticulum					250:258	endoplasmic reticulum	238:258	endoplasmic reticulum (ER)	238:263	SKN-1A/Nrf1, an endoplasmic reticulum (ER)-associated transcription factor that undergoes N-linked glycosylation, serves as a sensor of proteasome dysfunction and triggers compensatory upregulation of proteasome subunit genes.
31002798	2	41	theme	endoplasmic	238:248	arg1	ER					261:262	ER	261:262	ER	261:262	SKN-1A/Nrf1, an endoplasmic reticulum (ER)-associated transcription factor that undergoes N-linked glycosylation, serves as a sensor of proteasome dysfunction and triggers compensatory upregulation of proteasome subunit genes.
31002798	2	42	theme	proteasome	358:367	arg1	dysfunction					369:379	proteasome dysfunction	358:379	proteasome dysfunction	358:379	SKN-1A/Nrf1, an endoplasmic reticulum (ER)-associated transcription factor that undergoes N-linked glycosylation, serves as a sensor of proteasome dysfunction and triggers compensatory upregulation of proteasome subunit genes.
31002798	0	43	theme	Peptide	43:49	arg1	N-Glycanase					51:61	Peptide:N-Glycanase	43:61	Peptide:N-Glycanase	43:61	Protein Sequence Editing of SKN-1A/Nrf1 by Peptide:N-Glycanase Controls Proteasome Gene Expression.
31002798	6	44	theme	sequence	1037:1044	arg1	editing					1046:1052	Deglycosylation-dependent protein sequence editing	1003:1052	Deglycosylation-dependent protein sequence editing	1003:1052	Deglycosylation-dependent protein sequence editing explains how ER-associated and cytosolic isoforms of SKN-1 perform distinct cytoprotective functions corresponding to those of mammalian Nrf1 and Nrf2.
31002798	1	45	theme	selective	124:132	arg1	degradation					142:152	selective protein degradation	124:152	selective protein degradation	124:152	The proteasome mediates selective protein degradation and is dynamically regulated in response to proteotoxic challenges.
31002798	2	46	theme	transcription	276:288	arg1	factor					290:295	an endoplasmic reticulum (ER)-associated transcription factor	235:295	an endoplasmic reticulum (ER)-associated transcription factor that undergoes N-linked glycosylation	235:333	SKN-1A/Nrf1, an endoplasmic reticulum (ER)-associated transcription factor that undergoes N-linked glycosylation, serves as a sensor of proteasome dysfunction and triggers compensatory upregulation of proteasome subunit genes.
31002798	2	46	theme	transcription	276:288	arg1	SKN-1A/Nrf1					222:232	SKN-1A/Nrf1	222:232	SKN-1A/Nrf1	222:232	SKN-1A/Nrf1, an endoplasmic reticulum (ER)-associated transcription factor that undergoes N-linked glycosylation, serves as a sensor of proteasome dysfunction and triggers compensatory upregulation of proteasome subunit genes.
31002798	1	47	theme	protein	134:140	arg1	degradation					142:152	selective protein degradation	124:152	selective protein degradation	124:152	The proteasome mediates selective protein degradation and is dynamically regulated in response to proteotoxic challenges.
31002798	4	48	theme	N-glycosylation	663:677	arg1	sites					679:683	these N-glycosylation sites	657:683	these N-glycosylation sites	657:683	Genetically introducing aspartates at these N-glycosylation sites bypasses the requirement for PNG-1/NGLY1, showing that protein sequence editing rather than deglycosylation is key to SKN-1A function.
31002798	2	49	theme	-associated	264:274	arg1	factor					290:295	an endoplasmic reticulum (ER)-associated transcription factor	235:295	an endoplasmic reticulum (ER)-associated transcription factor that undergoes N-linked glycosylation	235:333	SKN-1A/Nrf1, an endoplasmic reticulum (ER)-associated transcription factor that undergoes N-linked glycosylation, serves as a sensor of proteasome dysfunction and triggers compensatory upregulation of proteasome subunit genes.
31002798	2	49	theme	-associated	264:274	arg1	SKN-1A/Nrf1					222:232	SKN-1A/Nrf1	222:232	SKN-1A/Nrf1	222:232	SKN-1A/Nrf1, an endoplasmic reticulum (ER)-associated transcription factor that undergoes N-linked glycosylation, serves as a sensor of proteasome dysfunction and triggers compensatory upregulation of proteasome subunit genes.
31002798	5	50	theme	amyloid	981:987	arg1	peptide					994:1000	human amyloid beta peptide	975:1000	human amyloid beta peptide	975:1000	This pathway is required to maintain sufficient proteasome expression and activity, and SKN-1A hyperactivation confers resistance to the proteotoxicity of human amyloid beta peptide.
31002798	7	51	link	N-linked	1256:1263	arg1	glycosylation					1265:1277	N-linked glycosylation	1256:1277	N-linked glycosylation	1256:1277	Thus, we uncover an unexpected mechanism by which N-linked glycosylation regulates protein function and proteostasis.
31002798	2	52	theme	compensatory	394:405	arg1	upregulation					407:418	compensatory upregulation	394:418	compensatory upregulation of proteasome subunit genes	394:446	SKN-1A/Nrf1, an endoplasmic reticulum (ER)-associated transcription factor that undergoes N-linked glycosylation, serves as a sensor of proteasome dysfunction and triggers compensatory upregulation of proteasome subunit genes.
31002798	2	52	theme	compensatory	394:405	arg1	SKN-1A/Nrf1					222:232	SKN-1A/Nrf1	222:232	SKN-1A/Nrf1	222:232	SKN-1A/Nrf1, an endoplasmic reticulum (ER)-associated transcription factor that undergoes N-linked glycosylation, serves as a sensor of proteasome dysfunction and triggers compensatory upregulation of proteasome subunit genes.
31002798	2	52	theme	compensatory	394:405	arg1	sensor					348:353	a sensor	346:353	a sensor of proteasome dysfunction	346:379	SKN-1A/Nrf1, an endoplasmic reticulum (ER)-associated transcription factor that undergoes N-linked glycosylation, serves as a sensor of proteasome dysfunction and triggers compensatory upregulation of proteasome subunit genes.
31002798	3	53	theme	asparagine	581:590	arg1	residues					592:599	particular N-glycosylated asparagine residues	555:599	particular N-glycosylated asparagine residues	555:599	Here, we show that the PNG-1/NGLY1 peptide:N-glycanase edits the sequence of SKN-1A protein by converting particular N-glycosylated asparagine residues to aspartic acid.
31002798	4	54	theme	sequence	748:755	arg1	editing					757:763	protein sequence editing	740:763	protein sequence editing rather than deglycosylation	740:791	Genetically introducing aspartates at these N-glycosylation sites bypasses the requirement for PNG-1/NGLY1, showing that protein sequence editing rather than deglycosylation is key to SKN-1A function.
31097254	4	0	theme	acid-like	886:894	arg1	components					896:905	three humic acid-like components	874:905	three humic acid-like components (C1 - C3)	874:915	The EPS was successfully categorized into three humic acid-like components (C1 - C3) and a protein-like component (C4).
31097254	1	1	theme	polymeric	166:174	arg1	substances					224:233	fundamental substances	212:233	fundamental substances in colony formation	212:253	Extracellular polymeric substances (EPS) were considered as fundamental substances in colony formation; however, the understanding of EPS composition remains limited.
31097254	1	1	theme	polymeric	166:174	arg1	EPS					188:190	EPS	188:190	EPS	188:190	Extracellular polymeric substances (EPS) were considered as fundamental substances in colony formation; however, the understanding of EPS composition remains limited.
31097254	1	1	theme	polymeric	166:174	arg1	substances					176:185	Extracellular polymeric substances	152:185	Extracellular polymeric substances (EPS)	152:191	Extracellular polymeric substances (EPS) were considered as fundamental substances in colony formation; however, the understanding of EPS composition remains limited.
31097254	8	2	theme	colony	1431:1436	arg1	formation					1438:1446	colony formation	1431:1446	colony formation	1431:1446	The tightly bound EPS directly promoted colony formation, but the soluble EPS or loosely bound EPS alone did not induce colony formation in Microcystis.
31097254	0	3	theme	substances	84:93	arg1	composition					101:111	Insights into the relationship between colony formation and extracellular polymeric substances (EPS) composition	0:111	Insights into the relationship between colony formation and extracellular polymeric substances (EPS) composition of the cyanobacterium Microcystis	0:145	Insights into the relationship between colony formation and extracellular polymeric substances (EPS) composition of the cyanobacterium Microcystis spp.
31097254	4	4	theme	humic	880:884	arg1	components					896:905	three humic acid-like components	874:905	three humic acid-like components (C1 - C3)	874:915	The EPS was successfully categorized into three humic acid-like components (C1 - C3) and a protein-like component (C4).
31097254	9	5	from	transformation	1715:1728	arg1	Microcystis					1772:1782	Microcystis	1772:1782	Microcystis	1772:1782	These results advanced the current knowledge on the chemical materials involved in the colony formation of Microcystis and provided new clues in unicellular-multicellular transformation as well as colonial morphology changes in Microcystis.
31097254	2	6	theme	Microcystis	577:587	arg1	blooms					589:594	Microcystis blooms	577:594	Microcystis blooms	577:594	This study analyzed the content and composition of EPS fractions (soluble EPS, loosely bound EPS, and tightly bound EPS) of four Microcystis species from laboratory cultures in both unicellular and colonial morphologies, as well as colonies collected during Microcystis blooms, using fluorescence excitation - emission matrix spectroscopy combined with parallel factor analysis (EEM-PARAFAC).
31097254	5	7	theme	Component	952:960	arg1	C1					962:963	Component C1	952:963	Component C1	952:963	Component C1 was discovered to be involved in colony formation and colony size growth of Microcystis.
31097254	2	8	theme	unicellular	501:511	arg1	morphologies					526:537	both unicellular and colonial morphologies	496:537	both unicellular and colonial morphologies	496:537	This study analyzed the content and composition of EPS fractions (soluble EPS, loosely bound EPS, and tightly bound EPS) of four Microcystis species from laboratory cultures in both unicellular and colonial morphologies, as well as colonies collected during Microcystis blooms, using fluorescence excitation - emission matrix spectroscopy combined with parallel factor analysis (EEM-PARAFAC).
31097254	2	9	theme	matrix	638:643	arg1	spectroscopy					645:656	fluorescence excitation - emission matrix spectroscopy	603:656	spectroscopy	645:656	This study analyzed the content and composition of EPS fractions (soluble EPS, loosely bound EPS, and tightly bound EPS) of four Microcystis species from laboratory cultures in both unicellular and colonial morphologies, as well as colonies collected during Microcystis blooms, using fluorescence excitation - emission matrix spectroscopy combined with parallel factor analysis (EEM-PARAFAC).
31097254	6	10	theme	Microcystis	1079:1089	arg1	morphospecies					1091:1103	Microcystis morphospecies	1079:1103	Microcystis morphospecies	1079:1103	EPS content varied among Microcystis morphospecies, such as M. aeruginosa, M. wesenbergii and M. ichthyoblabe, and this was significantly affected by the environmental constraints rather than the morphospecies.
31097254	6	10	theme	Microcystis	1079:1089	arg1	ichthyoblabe					1151:1162	M. ichthyoblabe	1148:1162	M. ichthyoblabe	1148:1162	EPS content varied among Microcystis morphospecies, such as M. aeruginosa, M. wesenbergii and M. ichthyoblabe, and this was significantly affected by the environmental constraints rather than the morphospecies.
31097254	6	10	theme	Microcystis	1079:1089	arg1	wesenbergii					1132:1142	M. wesenbergii	1129:1142	M. wesenbergii	1129:1142	EPS content varied among Microcystis morphospecies, such as M. aeruginosa, M. wesenbergii and M. ichthyoblabe, and this was significantly affected by the environmental constraints rather than the morphospecies.
31097254	6	10	theme	Microcystis	1079:1089	arg1	aeruginosa					1117:1126	M. aeruginosa	1114:1126	M. aeruginosa	1114:1126	EPS content varied among Microcystis morphospecies, such as M. aeruginosa, M. wesenbergii and M. ichthyoblabe, and this was significantly affected by the environmental constraints rather than the morphospecies.
31097254	9	11	theme	Microcystis	1651:1661	arg1	formation					1638:1646	the colony formation	1627:1646	the colony formation of Microcystis	1627:1661	These results advanced the current knowledge on the chemical materials involved in the colony formation of Microcystis and provided new clues in unicellular-multicellular transformation as well as colonial morphology changes in Microcystis.
31097254	2	12	theme	emission	629:636	arg1	spectroscopy					645:656	fluorescence excitation - emission matrix spectroscopy	603:656	spectroscopy	645:656	This study analyzed the content and composition of EPS fractions (soluble EPS, loosely bound EPS, and tightly bound EPS) of four Microcystis species from laboratory cultures in both unicellular and colonial morphologies, as well as colonies collected during Microcystis blooms, using fluorescence excitation - emission matrix spectroscopy combined with parallel factor analysis (EEM-PARAFAC).
31097254	2	13	dep	fractions	374:382	arg1	EPS					393:395	soluble EPS	385:395	soluble EPS	385:395	This study analyzed the content and composition of EPS fractions (soluble EPS, loosely bound EPS, and tightly bound EPS) of four Microcystis species from laboratory cultures in both unicellular and colonial morphologies, as well as colonies collected during Microcystis blooms, using fluorescence excitation - emission matrix spectroscopy combined with parallel factor analysis (EEM-PARAFAC).
31097254	2	13	dep	fractions	374:382	arg1	EPS					435:437	tightly bound EPS	421:437	tightly bound EPS	421:437	This study analyzed the content and composition of EPS fractions (soluble EPS, loosely bound EPS, and tightly bound EPS) of four Microcystis species from laboratory cultures in both unicellular and colonial morphologies, as well as colonies collected during Microcystis blooms, using fluorescence excitation - emission matrix spectroscopy combined with parallel factor analysis (EEM-PARAFAC).
31097254	2	13	dep	fractions	374:382	arg1	EPS					412:414	loosely bound EPS	398:414	loosely bound EPS	398:414	This study analyzed the content and composition of EPS fractions (soluble EPS, loosely bound EPS, and tightly bound EPS) of four Microcystis species from laboratory cultures in both unicellular and colonial morphologies, as well as colonies collected during Microcystis blooms, using fluorescence excitation - emission matrix spectroscopy combined with parallel factor analysis (EEM-PARAFAC).
31097254	6	14	theme	EPS	1054:1056	arg1	content					1058:1064	EPS content	1054:1064	EPS content	1054:1064	EPS content varied among Microcystis morphospecies, such as M. aeruginosa, M. wesenbergii and M. ichthyoblabe, and this was significantly affected by the environmental constraints rather than the morphospecies.
31097254	8	15	theme	colony	1511:1516	arg1	formation					1518:1526	colony formation	1511:1526	colony formation	1511:1526	The tightly bound EPS directly promoted colony formation, but the soluble EPS or loosely bound EPS alone did not induce colony formation in Microcystis.
31097254	0	16	theme	colony	39:44	arg1	formation					46:54	colony formation	39:54	colony formation	39:54	Insights into the relationship between colony formation and extracellular polymeric substances (EPS) composition of the cyanobacterium Microcystis spp.
31097254	2	17	theme	bound	429:433	arg1	EPS					435:437	tightly bound EPS	421:437	tightly bound EPS	421:437	This study analyzed the content and composition of EPS fractions (soluble EPS, loosely bound EPS, and tightly bound EPS) of four Microcystis species from laboratory cultures in both unicellular and colonial morphologies, as well as colonies collected during Microcystis blooms, using fluorescence excitation - emission matrix spectroscopy combined with parallel factor analysis (EEM-PARAFAC).
31097254	2	18	theme	species	460:466	arg1	fractions					374:382	EPS fractions	370:382	EPS fractions (soluble EPS, loosely bound EPS, and tightly bound EPS) of four Microcystis species from laboratory cultures in both unicellular and colonial morphologies	370:537	This study analyzed the content and composition of EPS fractions (soluble EPS, loosely bound EPS, and tightly bound EPS) of four Microcystis species from laboratory cultures in both unicellular and colonial morphologies, as well as colonies collected during Microcystis blooms, using fluorescence excitation - emission matrix spectroscopy combined with parallel factor analysis (EEM-PARAFAC).
31097254	2	19	theme	fluorescence	603:614	arg1	excitation					616:625	fluorescence excitation - emission matrix spectroscopy	603:656	excitation	616:625	This study analyzed the content and composition of EPS fractions (soluble EPS, loosely bound EPS, and tightly bound EPS) of four Microcystis species from laboratory cultures in both unicellular and colonial morphologies, as well as colonies collected during Microcystis blooms, using fluorescence excitation - emission matrix spectroscopy combined with parallel factor analysis (EEM-PARAFAC).
31097254	2	20	theme	factor	681:686	arg1	EEM-PARAFAC					698:708	EEM-PARAFAC	698:708	EEM-PARAFAC	698:708	This study analyzed the content and composition of EPS fractions (soluble EPS, loosely bound EPS, and tightly bound EPS) of four Microcystis species from laboratory cultures in both unicellular and colonial morphologies, as well as colonies collected during Microcystis blooms, using fluorescence excitation - emission matrix spectroscopy combined with parallel factor analysis (EEM-PARAFAC).
31097254	2	20	theme	factor	681:686	arg1	analysis					688:695	parallel factor analysis	672:695	parallel factor analysis (EEM-PARAFAC)	672:709	This study analyzed the content and composition of EPS fractions (soluble EPS, loosely bound EPS, and tightly bound EPS) of four Microcystis species from laboratory cultures in both unicellular and colonial morphologies, as well as colonies collected during Microcystis blooms, using fluorescence excitation - emission matrix spectroscopy combined with parallel factor analysis (EEM-PARAFAC).
31097254	2	21	theme	soluble	385:391	arg1	EPS					393:395	soluble EPS	385:395	soluble EPS	385:395	This study analyzed the content and composition of EPS fractions (soluble EPS, loosely bound EPS, and tightly bound EPS) of four Microcystis species from laboratory cultures in both unicellular and colonial morphologies, as well as colonies collected during Microcystis blooms, using fluorescence excitation - emission matrix spectroscopy combined with parallel factor analysis (EEM-PARAFAC).
31097254	0	22	theme	Insights	0:7	arg1	composition					101:111	Insights into the relationship between colony formation and extracellular polymeric substances (EPS) composition	0:111	Insights into the relationship between colony formation and extracellular polymeric substances (EPS) composition of the cyanobacterium Microcystis	0:145	Insights into the relationship between colony formation and extracellular polymeric substances (EPS) composition of the cyanobacterium Microcystis spp.
31097254	9	23	theme	new	1676:1678	arg1	clues					1680:1684	new clues	1676:1684	new clues	1676:1684	These results advanced the current knowledge on the chemical materials involved in the colony formation of Microcystis and provided new clues in unicellular-multicellular transformation as well as colonial morphology changes in Microcystis.
31097254	2	24	theme	parallel	672:679	arg1	EEM-PARAFAC					698:708	EEM-PARAFAC	698:708	EEM-PARAFAC	698:708	This study analyzed the content and composition of EPS fractions (soluble EPS, loosely bound EPS, and tightly bound EPS) of four Microcystis species from laboratory cultures in both unicellular and colonial morphologies, as well as colonies collected during Microcystis blooms, using fluorescence excitation - emission matrix spectroscopy combined with parallel factor analysis (EEM-PARAFAC).
31097254	2	24	theme	parallel	672:679	arg1	analysis					688:695	parallel factor analysis	672:695	parallel factor analysis (EEM-PARAFAC)	672:709	This study analyzed the content and composition of EPS fractions (soluble EPS, loosely bound EPS, and tightly bound EPS) of four Microcystis species from laboratory cultures in both unicellular and colonial morphologies, as well as colonies collected during Microcystis blooms, using fluorescence excitation - emission matrix spectroscopy combined with parallel factor analysis (EEM-PARAFAC).
31097254	8	25	theme	soluble	1457:1463	arg1	EPS					1465:1467	the soluble EPS	1453:1467	the soluble EPS	1453:1467	The tightly bound EPS directly promoted colony formation, but the soluble EPS or loosely bound EPS alone did not induce colony formation in Microcystis.
31097254	7	26	theme	C1	1283:1284	arg1	proportion					1269:1278	The proportion	1265:1278	The proportion of C1 relating to larger colony size	1265:1315	The proportion of C1 relating to larger colony size was negatively correlated to temperature and concentrations of TN and TP.
31097254	2	27	from	fractions	374:382	arg1	cultures					484:491	laboratory cultures	473:491	laboratory cultures in both unicellular and colonial morphologies	473:537	This study analyzed the content and composition of EPS fractions (soluble EPS, loosely bound EPS, and tightly bound EPS) of four Microcystis species from laboratory cultures in both unicellular and colonial morphologies, as well as colonies collected during Microcystis blooms, using fluorescence excitation - emission matrix spectroscopy combined with parallel factor analysis (EEM-PARAFAC).
31097254	1	28	theme	EPS	286:288	arg1	composition					290:300	EPS composition	286:300	EPS composition	286:300	Extracellular polymeric substances (EPS) were considered as fundamental substances in colony formation; however, the understanding of EPS composition remains limited.
31097254	0	29	theme	cyanobacterium	120:133	arg1	Microcystis					135:145	the cyanobacterium Microcystis	116:145	the cyanobacterium Microcystis	116:145	Insights into the relationship between colony formation and extracellular polymeric substances (EPS) composition of the cyanobacterium Microcystis spp.
31097254	7	30	theme	TP	1387:1388	arg1	temperature					1346:1356	temperature	1346:1356	temperature	1346:1356	The proportion of C1 relating to larger colony size was negatively correlated to temperature and concentrations of TN and TP.
31097254	7	30	theme	TP	1387:1388	arg1	concentrations					1362:1375	concentrations	1362:1375	concentrations of TN and TP	1362:1388	The proportion of C1 relating to larger colony size was negatively correlated to temperature and concentrations of TN and TP.
31097254	7	31	theme	TN	1380:1381	arg1	temperature					1346:1356	temperature	1346:1356	temperature	1346:1356	The proportion of C1 relating to larger colony size was negatively correlated to temperature and concentrations of TN and TP.
31097254	7	31	theme	TN	1380:1381	arg1	concentrations					1362:1375	concentrations	1362:1375	concentrations of TN and TP	1362:1388	The proportion of C1 relating to larger colony size was negatively correlated to temperature and concentrations of TN and TP.
31097254	1	32	theme	composition	290:300	arg1	understanding					269:281	the understanding	265:281	the understanding of EPS composition	265:300	Extracellular polymeric substances (EPS) were considered as fundamental substances in colony formation; however, the understanding of EPS composition remains limited.
31097254	4	33	theme	protein-like	923:934	arg1	C4					947:948	C4	947:948	C4	947:948	The EPS was successfully categorized into three humic acid-like components (C1 - C3) and a protein-like component (C4).
31097254	4	33	theme	protein-like	923:934	arg1	component					936:944	a protein-like component	921:944	a protein-like component (C4)	921:949	The EPS was successfully categorized into three humic acid-like components (C1 - C3) and a protein-like component (C4).
31097254	7	34	theme	colony	1305:1310	arg1	size					1312:1315	larger colony size	1298:1315	larger colony size	1298:1315	The proportion of C1 relating to larger colony size was negatively correlated to temperature and concentrations of TN and TP.
31097254	7	35	theme	larger	1298:1303	arg1	size					1312:1315	larger colony size	1298:1315	larger colony size	1298:1315	The proportion of C1 relating to larger colony size was negatively correlated to temperature and concentrations of TN and TP.
31097254	2	36	theme	Microcystis	448:458	arg1	species					460:466	four Microcystis species	443:466	four Microcystis species from laboratory cultures in both unicellular and colonial morphologies	443:537	This study analyzed the content and composition of EPS fractions (soluble EPS, loosely bound EPS, and tightly bound EPS) of four Microcystis species from laboratory cultures in both unicellular and colonial morphologies, as well as colonies collected during Microcystis blooms, using fluorescence excitation - emission matrix spectroscopy combined with parallel factor analysis (EEM-PARAFAC).
31097254	9	37	theme	unicellular-multicellular	1689:1713	arg1	transformation					1715:1728	unicellular-multicellular transformation	1689:1728	unicellular-multicellular transformation as well as colonial morphology changes in Microcystis	1689:1782	These results advanced the current knowledge on the chemical materials involved in the colony formation of Microcystis and provided new clues in unicellular-multicellular transformation as well as colonial morphology changes in Microcystis.
31097254	2	38	theme	bound	406:410	arg1	EPS					412:414	loosely bound EPS	398:414	loosely bound EPS	398:414	This study analyzed the content and composition of EPS fractions (soluble EPS, loosely bound EPS, and tightly bound EPS) of four Microcystis species from laboratory cultures in both unicellular and colonial morphologies, as well as colonies collected during Microcystis blooms, using fluorescence excitation - emission matrix spectroscopy combined with parallel factor analysis (EEM-PARAFAC).
31097254	5	39	theme	colony	998:1003	arg1	formation					1005:1013	colony formation	998:1013	colony formation	998:1013	Component C1 was discovered to be involved in colony formation and colony size growth of Microcystis.
31097254	1	40	theme	fundamental	212:222	arg1	substances					176:185	Extracellular polymeric substances	152:185	Extracellular polymeric substances (EPS)	152:191	Extracellular polymeric substances (EPS) were considered as fundamental substances in colony formation; however, the understanding of EPS composition remains limited.
31097254	1	40	theme	fundamental	212:222	arg1	substances					224:233	fundamental substances	212:233	fundamental substances in colony formation	212:253	Extracellular polymeric substances (EPS) were considered as fundamental substances in colony formation; however, the understanding of EPS composition remains limited.
31097254	3	41	theme	protein-like	753:764	arg1	components					786:795	protein-like and humic acid-like components	753:795	protein-like and humic acid-like components	753:795	This method enables to make insight into protein-like and humic acid-like components but cannot detect polysaccharides.
31097254	3	42	theme	acid-like	776:784	arg1	components					786:795	protein-like and humic acid-like components	753:795	protein-like and humic acid-like components	753:795	This method enables to make insight into protein-like and humic acid-like components but cannot detect polysaccharides.
31097254	0	43	theme	Microcystis	135:145	arg1	composition					101:111	Insights into the relationship between colony formation and extracellular polymeric substances (EPS) composition	0:111	Insights into the relationship between colony formation and extracellular polymeric substances (EPS) composition of the cyanobacterium Microcystis	0:145	Insights into the relationship between colony formation and extracellular polymeric substances (EPS) composition of the cyanobacterium Microcystis spp.
31097254	2	44	from	cultures	484:491	arg1	species					460:466	four Microcystis species	443:466	four Microcystis species from laboratory cultures in both unicellular and colonial morphologies	443:537	This study analyzed the content and composition of EPS fractions (soluble EPS, loosely bound EPS, and tightly bound EPS) of four Microcystis species from laboratory cultures in both unicellular and colonial morphologies, as well as colonies collected during Microcystis blooms, using fluorescence excitation - emission matrix spectroscopy combined with parallel factor analysis (EEM-PARAFAC).
31097254	2	44	from	cultures	484:491	arg1	morphologies					526:537	both unicellular and colonial morphologies	496:537	both unicellular and colonial morphologies	496:537	This study analyzed the content and composition of EPS fractions (soluble EPS, loosely bound EPS, and tightly bound EPS) of four Microcystis species from laboratory cultures in both unicellular and colonial morphologies, as well as colonies collected during Microcystis blooms, using fluorescence excitation - emission matrix spectroscopy combined with parallel factor analysis (EEM-PARAFAC).
31097254	2	44	from	cultures	484:491	arg1	fractions					374:382	EPS fractions	370:382	EPS fractions (soluble EPS, loosely bound EPS, and tightly bound EPS) of four Microcystis species from laboratory cultures in both unicellular and colonial morphologies	370:537	This study analyzed the content and composition of EPS fractions (soluble EPS, loosely bound EPS, and tightly bound EPS) of four Microcystis species from laboratory cultures in both unicellular and colonial morphologies, as well as colonies collected during Microcystis blooms, using fluorescence excitation - emission matrix spectroscopy combined with parallel factor analysis (EEM-PARAFAC).
31097254	6	45	theme	environmental	1208:1220	arg1	constraints					1222:1232	the environmental constraints	1204:1232	the environmental constraints rather than the morphospecies	1204:1262	EPS content varied among Microcystis morphospecies, such as M. aeruginosa, M. wesenbergii and M. ichthyoblabe, and this was significantly affected by the environmental constraints rather than the morphospecies.
31097254	9	46	dep	materials	1605:1613	arg1	involved					1615:1622	involved	1615:1622	involved in the colony formation of Microcystis	1615:1661	These results advanced the current knowledge on the chemical materials involved in the colony formation of Microcystis and provided new clues in unicellular-multicellular transformation as well as colonial morphology changes in Microcystis.
31097254	9	46	dep	materials	1605:1613	arg1	provided					1667:1674	provided	1667:1674	provided new clues in unicellular-multicellular transformation as well as colonial morphology changes in Microcystis	1667:1782	These results advanced the current knowledge on the chemical materials involved in the colony formation of Microcystis and provided new clues in unicellular-multicellular transformation as well as colonial morphology changes in Microcystis.
31097254	2	47	theme	laboratory	473:482	arg1	cultures					484:491	laboratory cultures	473:491	laboratory cultures in both unicellular and colonial morphologies	473:537	This study analyzed the content and composition of EPS fractions (soluble EPS, loosely bound EPS, and tightly bound EPS) of four Microcystis species from laboratory cultures in both unicellular and colonial morphologies, as well as colonies collected during Microcystis blooms, using fluorescence excitation - emission matrix spectroscopy combined with parallel factor analysis (EEM-PARAFAC).
31097254	9	48	from	changes	1761:1767	arg1	Microcystis					1772:1782	Microcystis	1772:1782	Microcystis	1772:1782	These results advanced the current knowledge on the chemical materials involved in the colony formation of Microcystis and provided new clues in unicellular-multicellular transformation as well as colonial morphology changes in Microcystis.
31097254	1	49	from	substances	224:233	arg1	formation					245:253	colony formation	238:253	colony formation	238:253	Extracellular polymeric substances (EPS) were considered as fundamental substances in colony formation; however, the understanding of EPS composition remains limited.
31097254	2	50	theme	colonial	517:524	arg1	morphologies					526:537	both unicellular and colonial morphologies	496:537	both unicellular and colonial morphologies	496:537	This study analyzed the content and composition of EPS fractions (soluble EPS, loosely bound EPS, and tightly bound EPS) of four Microcystis species from laboratory cultures in both unicellular and colonial morphologies, as well as colonies collected during Microcystis blooms, using fluorescence excitation - emission matrix spectroscopy combined with parallel factor analysis (EEM-PARAFAC).
31097254	5	51	theme	size	1026:1029	arg1	growth					1031:1036	colony size growth	1019:1036	colony size growth	1019:1036	Component C1 was discovered to be involved in colony formation and colony size growth of Microcystis.
31097254	5	52	theme	colony	1019:1024	arg1	growth					1031:1036	colony size growth	1019:1036	colony size growth	1019:1036	Component C1 was discovered to be involved in colony formation and colony size growth of Microcystis.
31097254	9	53	theme	colony	1631:1636	arg1	formation					1638:1646	the colony formation	1627:1646	the colony formation of Microcystis	1627:1661	These results advanced the current knowledge on the chemical materials involved in the colony formation of Microcystis and provided new clues in unicellular-multicellular transformation as well as colonial morphology changes in Microcystis.
31097254	4	54	dep	components	896:905	arg1	C3					913:914	C3	913:914	C3	913:914	The EPS was successfully categorized into three humic acid-like components (C1 - C3) and a protein-like component (C4).
31097254	4	54	dep	components	896:905	arg1	C1					908:909	C1	908:909	C1	908:909	The EPS was successfully categorized into three humic acid-like components (C1 - C3) and a protein-like component (C4).
31097254	3	55	theme	humic	770:774	arg1	components					786:795	protein-like and humic acid-like components	753:795	protein-like and humic acid-like components	753:795	This method enables to make insight into protein-like and humic acid-like components but cannot detect polysaccharides.
31097254	9	56	theme	morphology	1750:1759	arg1	changes					1761:1767	colonial morphology changes	1741:1767	unicellular-multicellular transformation as well as colonial morphology changes in Microcystis	1689:1782	These results advanced the current knowledge on the chemical materials involved in the colony formation of Microcystis and provided new clues in unicellular-multicellular transformation as well as colonial morphology changes in Microcystis.
31097254	9	57	theme	chemical	1596:1603	arg1	materials					1605:1613	the chemical materials	1592:1613	the chemical materials involved in the colony formation of Microcystis and provided new clues in unicellular-multicellular transformation as well as colonial morphology changes in Microcystis	1592:1782	These results advanced the current knowledge on the chemical materials involved in the colony formation of Microcystis and provided new clues in unicellular-multicellular transformation as well as colonial morphology changes in Microcystis.
31097254	9	58	theme	colonial	1741:1748	arg1	changes					1761:1767	colonial morphology changes	1741:1767	unicellular-multicellular transformation as well as colonial morphology changes in Microcystis	1689:1782	These results advanced the current knowledge on the chemical materials involved in the colony formation of Microcystis and provided new clues in unicellular-multicellular transformation as well as colonial morphology changes in Microcystis.
31097254	8	59	theme	bound	1403:1407	arg1	EPS					1409:1411	The tightly bound EPS	1391:1411	The tightly bound EPS	1391:1411	The tightly bound EPS directly promoted colony formation, but the soluble EPS or loosely bound EPS alone did not induce colony formation in Microcystis.
31097254	2	60	theme	EPS	370:372	arg1	fractions					374:382	EPS fractions	370:382	EPS fractions (soluble EPS, loosely bound EPS, and tightly bound EPS) of four Microcystis species from laboratory cultures in both unicellular and colonial morphologies	370:537	This study analyzed the content and composition of EPS fractions (soluble EPS, loosely bound EPS, and tightly bound EPS) of four Microcystis species from laboratory cultures in both unicellular and colonial morphologies, as well as colonies collected during Microcystis blooms, using fluorescence excitation - emission matrix spectroscopy combined with parallel factor analysis (EEM-PARAFAC).
31097254	1	61	theme	colony	238:243	arg1	formation					245:253	colony formation	238:253	colony formation	238:253	Extracellular polymeric substances (EPS) were considered as fundamental substances in colony formation; however, the understanding of EPS composition remains limited.
31097254	0	62	theme	polymeric	74:82	arg1	substances					84:93	extracellular polymeric substances	60:93	extracellular polymeric substances (EPS)	60:99	Insights into the relationship between colony formation and extracellular polymeric substances (EPS) composition of the cyanobacterium Microcystis spp.
31097254	0	62	theme	polymeric	74:82	arg1	EPS					96:98	EPS	96:98	EPS	96:98	Insights into the relationship between colony formation and extracellular polymeric substances (EPS) composition of the cyanobacterium Microcystis spp.
31097254	5	63	theme	Microcystis	1041:1051	arg1	formation					1005:1013	colony formation	998:1013	colony formation	998:1013	Component C1 was discovered to be involved in colony formation and colony size growth of Microcystis.
31097254	5	63	theme	Microcystis	1041:1051	arg1	growth					1031:1036	colony size growth	1019:1036	colony size growth	1019:1036	Component C1 was discovered to be involved in colony formation and colony size growth of Microcystis.
31097254	2	64	theme	fractions	374:382	arg1	content					343:349	the content	339:349	the content	339:349	This study analyzed the content and composition of EPS fractions (soluble EPS, loosely bound EPS, and tightly bound EPS) of four Microcystis species from laboratory cultures in both unicellular and colonial morphologies, as well as colonies collected during Microcystis blooms, using fluorescence excitation - emission matrix spectroscopy combined with parallel factor analysis (EEM-PARAFAC).
31097254	2	64	theme	fractions	374:382	arg1	colonies					551:558	colonies	551:558	colonies collected during Microcystis blooms	551:594	This study analyzed the content and composition of EPS fractions (soluble EPS, loosely bound EPS, and tightly bound EPS) of four Microcystis species from laboratory cultures in both unicellular and colonial morphologies, as well as colonies collected during Microcystis blooms, using fluorescence excitation - emission matrix spectroscopy combined with parallel factor analysis (EEM-PARAFAC).
31097254	2	64	theme	fractions	374:382	arg1	composition					355:365	composition	355:365	composition	355:365	This study analyzed the content and composition of EPS fractions (soluble EPS, loosely bound EPS, and tightly bound EPS) of four Microcystis species from laboratory cultures in both unicellular and colonial morphologies, as well as colonies collected during Microcystis blooms, using fluorescence excitation - emission matrix spectroscopy combined with parallel factor analysis (EEM-PARAFAC).
31097254	9	65	theme	current	1571:1577	arg1	knowledge					1579:1587	the current knowledge	1567:1587	the current knowledge	1567:1587	These results advanced the current knowledge on the chemical materials involved in the colony formation of Microcystis and provided new clues in unicellular-multicellular transformation as well as colonial morphology changes in Microcystis.
31097254	0	66	theme	extracellular	60:72	arg1	substances					84:93	extracellular polymeric substances	60:93	extracellular polymeric substances (EPS)	60:99	Insights into the relationship between colony formation and extracellular polymeric substances (EPS) composition of the cyanobacterium Microcystis spp.
31097254	0	66	theme	extracellular	60:72	arg1	EPS					96:98	EPS	96:98	EPS	96:98	Insights into the relationship between colony formation and extracellular polymeric substances (EPS) composition of the cyanobacterium Microcystis spp.
31097254	8	67	theme	bound	1480:1484	arg1	EPS					1486:1488	loosely bound EPS	1472:1488	loosely bound EPS	1472:1488	The tightly bound EPS directly promoted colony formation, but the soluble EPS or loosely bound EPS alone did not induce colony formation in Microcystis.
31097254	1	68	theme	Extracellular	152:164	arg1	substances					224:233	fundamental substances	212:233	fundamental substances in colony formation	212:253	Extracellular polymeric substances (EPS) were considered as fundamental substances in colony formation; however, the understanding of EPS composition remains limited.
31097254	1	68	theme	Extracellular	152:164	arg1	EPS					188:190	EPS	188:190	EPS	188:190	Extracellular polymeric substances (EPS) were considered as fundamental substances in colony formation; however, the understanding of EPS composition remains limited.
31097254	1	68	theme	Extracellular	152:164	arg1	substances					176:185	Extracellular polymeric substances	152:185	Extracellular polymeric substances (EPS)	152:191	Extracellular polymeric substances (EPS) were considered as fundamental substances in colony formation; however, the understanding of EPS composition remains limited.
29741879	7	0	theme	glycopeptides	1561:1573	arg1	identification					1537:1550	the identification	1533:1550	the identification of 2 683 glycopeptides from the global proteomic data set and 4 554 glycopeptides from phosphoproteomic data set	1533:1663	Reanalysis of the CPTAC data set resulted in the identification of 2 683 glycopeptides from the global proteomic data set and 4 554 glycopeptides from phosphoproteomic data set, respectively.
29741879	10	1	theme	protein	2223:2229	arg1	glycosylation					2231:2243	protein glycosylation	2223:2243	protein glycosylation	2223:2243	The reanalysis described herein can be readily applied to identify glycopeptides from already existing data sets, providing insight into many important facets of protein glycosylation in different biological, physiological, and pathological processes.
29741879	3	2	theme	glycopeptides	702:714	arg1	number					659:664	a large number	651:664	a large number of previously unidentified N-linked glycopeptides	651:714	In this study, we describe the reanalysis of global proteomic data from breast cancer xenograft tissues using recently developed software package GPQuest 2.0, revealing a large number of previously unidentified N-linked glycopeptides.
29741879	6	3	theme	data	1355:1358	arg1	reanalysis					1313:1322	the reanalysis	1309:1322	the reanalysis of global and phosphoproteomic data generated from 108 human breast cancer tissues that were previously analyzed by Clinical Proteomic Analysis Consortium (CPTAC)	1309:1485	Next, we utilized GPQuest 2.0 for the reanalysis of global and phosphoproteomic data generated from 108 human breast cancer tissues that were previously analyzed by Clinical Proteomic Analysis Consortium (CPTAC).
29741879	2	4	theme	glycosylation	354:366	arg1	role					346:349	the vital role	336:349	the vital role of glycosylation for proper protein function	336:394	Despite the vital role of glycosylation for proper protein function, the analysis of glycoproteins has been lagged behind to other protein modifications.
29741879	7	5	theme	CPTAC	1506:1510	arg1	set					1517:1519	the CPTAC data set	1502:1519	the CPTAC data set	1502:1519	Reanalysis of the CPTAC data set resulted in the identification of 2 683 glycopeptides from the global proteomic data set and 4 554 glycopeptides from phosphoproteomic data set, respectively.
29741879	3	6	from	reanalysis	513:522	arg1	tissues					578:584	breast cancer xenograft tissues	554:584	breast cancer xenograft tissues using recently developed software package GPQuest 2.0	554:638	In this study, we describe the reanalysis of global proteomic data from breast cancer xenograft tissues using recently developed software package GPQuest 2.0, revealing a large number of previously unidentified N-linked glycopeptides.
29741879	4	7	theme	substantial	870:880	arg1	number					882:887	a substantial number	868:887	a substantial number	868:887	More importantly, we found that using immobilized metal affinity chromatography (IMAC) technology for the enrichment of phosphopeptides had coenriched a substantial number of sialoglycopeptides, allowing for a large-scale analysis of sialoglycopeptides in conjunction with the analysis of phosphopeptides.
29741879	7	8	gly	glycopeptides	1620:1632	arg2	glycopeptides					1620:1632	4 554 glycopeptides	1614:1632	4 554 glycopeptides from phosphoproteomic data set	1614:1663	Reanalysis of the CPTAC data set resulted in the identification of 2 683 glycopeptides from the global proteomic data set and 4 554 glycopeptides from phosphoproteomic data set, respectively.
29741879	7	8	gly	glycopeptides	1620:1632	arg1	set					1661:1663	phosphoproteomic data set	1639:1663	phosphoproteomic data set	1639:1663	Reanalysis of the CPTAC data set resulted in the identification of 2 683 glycopeptides from the global proteomic data set and 4 554 glycopeptides from phosphoproteomic data set, respectively.
29741879	7	8	gly	glycopeptides	1620:1632	arg1	set					1606:1608	the global proteomic data set	1580:1608	the global proteomic data set	1580:1608	Reanalysis of the CPTAC data set resulted in the identification of 2 683 glycopeptides from the global proteomic data set and 4 554 glycopeptides from phosphoproteomic data set, respectively.
29741879	10	9	theme	biological	2258:2267	arg1	processes					2302:2310	different biological, physiological, and pathological processes	2248:2310	different biological, physiological, and pathological processes	2248:2310	The reanalysis described herein can be readily applied to identify glycopeptides from already existing data sets, providing insight into many important facets of protein glycosylation in different biological, physiological, and pathological processes.
29741879	3	10	theme	proteomic	534:542	arg1	data					544:547	global proteomic data	527:547	global proteomic data from breast cancer xenograft tissues using recently developed software package GPQuest 2.0	527:638	In this study, we describe the reanalysis of global proteomic data from breast cancer xenograft tissues using recently developed software package GPQuest 2.0, revealing a large number of previously unidentified N-linked glycopeptides.
29741879	8	11	theme	data	1821:1824	arg1	sets					1826:1829	the two data sets	1813:1829	the two data sets	1813:1829	Together, 11 292 N-linked glycopeptides corresponding to 1 731 N-linked glycosites from 883 human glycoproteins were identified from the two data sets.
29741879	1	12	theme	previous	179:186	arg1	reports					188:194	previous reports	179:194	previous reports	179:194	Protein glycosylation plays fundamental roles in many cellular processes, and previous reports have shown dysregulation to be associated with several human diseases, including diabetes, cancer, and neurodegenerative disorders.
29741879	5	13	attach	derived	1225:1231	arg2	glycoproteins					1211:1223	617 glycoproteins	1207:1223	617 glycoproteins derived from two breast cancer xenograft tissues	1207:1272	Collectively, combined tandem mass spectrometry (MS/MS) analyses of global proteomic and phosphoproteomic data sets resulted in the identification of 6 724 N-linked glycopeptides from 617 glycoproteins derived from two breast cancer xenograft tissues.
29741879	5	13	attach	derived	1225:1231	arg1	tissues					1266:1272	two breast cancer xenograft tissues	1238:1272	two breast cancer xenograft tissues	1238:1272	Collectively, combined tandem mass spectrometry (MS/MS) analyses of global proteomic and phosphoproteomic data sets resulted in the identification of 6 724 N-linked glycopeptides from 617 glycoproteins derived from two breast cancer xenograft tissues.
29741879	8	14	gly	glycosites	1752:1761	arg2	glycosites					1752:1761	1 731 N-linked glycosites	1737:1761	1 731 N-linked glycosites from 883 human glycoproteins	1737:1790	Together, 11 292 N-linked glycopeptides corresponding to 1 731 N-linked glycosites from 883 human glycoproteins were identified from the two data sets.
29741879	10	15	gly	glycopeptides	2128:2140	arg2	glycopeptides					2128:2140	glycopeptides	2128:2140	glycopeptides from already existing data sets	2128:2172	The reanalysis described herein can be readily applied to identify glycopeptides from already existing data sets, providing insight into many important facets of protein glycosylation in different biological, physiological, and pathological processes.
29741879	10	15	gly	glycopeptides	2128:2140	arg1	sets					2169:2172	already existing data sets	2147:2172	already existing data sets	2147:2172	The reanalysis described herein can be readily applied to identify glycopeptides from already existing data sets, providing insight into many important facets of protein glycosylation in different biological, physiological, and pathological processes.
29741879	5	16	theme	data	1129:1132	arg1	sets					1134:1137	global proteomic and phosphoproteomic data sets	1091:1137	global proteomic and phosphoproteomic data sets	1091:1137	Collectively, combined tandem mass spectrometry (MS/MS) analyses of global proteomic and phosphoproteomic data sets resulted in the identification of 6 724 N-linked glycopeptides from 617 glycoproteins derived from two breast cancer xenograft tissues.
29741879	3	17	theme	cancer	561:566	arg1	tissues					578:584	breast cancer xenograft tissues	554:584	breast cancer xenograft tissues using recently developed software package GPQuest 2.0	554:638	In this study, we describe the reanalysis of global proteomic data from breast cancer xenograft tissues using recently developed software package GPQuest 2.0, revealing a large number of previously unidentified N-linked glycopeptides.
29741879	7	18	from	identification	1537:1550	arg1	set					1606:1608	the global proteomic data set	1580:1608	the global proteomic data set	1580:1608	Reanalysis of the CPTAC data set resulted in the identification of 2 683 glycopeptides from the global proteomic data set and 4 554 glycopeptides from phosphoproteomic data set, respectively.
29741879	7	18	from	identification	1537:1550	arg1	set					1661:1663	phosphoproteomic data set	1639:1663	phosphoproteomic data set	1639:1663	Reanalysis of the CPTAC data set resulted in the identification of 2 683 glycopeptides from the global proteomic data set and 4 554 glycopeptides from phosphoproteomic data set, respectively.
29741879	1	19	theme	fundamental	129:139	arg1	roles					141:145	fundamental roles	129:145	fundamental roles	129:145	Protein glycosylation plays fundamental roles in many cellular processes, and previous reports have shown dysregulation to be associated with several human diseases, including diabetes, cancer, and neurodegenerative disorders.
29741879	8	20	link	N-linked	1697:1704	arg1	glycopeptides					1706:1718	11 292 N-linked glycopeptides	1690:1718	11 292 N-linked glycopeptides corresponding to 1 731 N-linked glycosites from 883 human glycoproteins	1690:1790	Together, 11 292 N-linked glycopeptides corresponding to 1 731 N-linked glycosites from 883 human glycoproteins were identified from the two data sets.
29741879	10	21	theme	physiological	2270:2282	arg1	processes					2302:2310	different biological, physiological, and pathological processes	2248:2310	different biological, physiological, and pathological processes	2248:2310	The reanalysis described herein can be readily applied to identify glycopeptides from already existing data sets, providing insight into many important facets of protein glycosylation in different biological, physiological, and pathological processes.
29741879	6	22	theme	cancer	1392:1397	arg1	tissues					1399:1405	108 human breast cancer tissues	1375:1405	108 human breast cancer tissues that were previously analyzed by Clinical Proteomic Analysis Consortium (CPTAC)	1375:1485	Next, we utilized GPQuest 2.0 for the reanalysis of global and phosphoproteomic data generated from 108 human breast cancer tissues that were previously analyzed by Clinical Proteomic Analysis Consortium (CPTAC).
29741879	3	23	theme	software	611:618	arg1	package					620:626	recently developed software package GPQuest 2.0	592:638	recently developed software package GPQuest 2.0	592:638	In this study, we describe the reanalysis of global proteomic data from breast cancer xenograft tissues using recently developed software package GPQuest 2.0, revealing a large number of previously unidentified N-linked glycopeptides.
29741879	4	24	theme	large-scale	927:937	arg1	analysis					939:946	a large-scale analysis	925:946	a large-scale analysis of sialoglycopeptides in conjunction with the analysis of phosphopeptides	925:1020	More importantly, we found that using immobilized metal affinity chromatography (IMAC) technology for the enrichment of phosphopeptides had coenriched a substantial number of sialoglycopeptides, allowing for a large-scale analysis of sialoglycopeptides in conjunction with the analysis of phosphopeptides.
29741879	6	25	theme	human	1379:1383	arg1	tissues					1399:1405	108 human breast cancer tissues	1375:1405	108 human breast cancer tissues that were previously analyzed by Clinical Proteomic Analysis Consortium (CPTAC)	1375:1485	Next, we utilized GPQuest 2.0 for the reanalysis of global and phosphoproteomic data generated from 108 human breast cancer tissues that were previously analyzed by Clinical Proteomic Analysis Consortium (CPTAC).
29741879	5	26	theme	global	1091:1096	arg1	sets					1134:1137	global proteomic and phosphoproteomic data sets	1091:1137	global proteomic and phosphoproteomic data sets	1091:1137	Collectively, combined tandem mass spectrometry (MS/MS) analyses of global proteomic and phosphoproteomic data sets resulted in the identification of 6 724 N-linked glycopeptides from 617 glycoproteins derived from two breast cancer xenograft tissues.
29741879	4	27	from	analysis	939:946	arg1	conjunction					973:983	conjunction	973:983	conjunction with the analysis of phosphopeptides	973:1020	More importantly, we found that using immobilized metal affinity chromatography (IMAC) technology for the enrichment of phosphopeptides had coenriched a substantial number of sialoglycopeptides, allowing for a large-scale analysis of sialoglycopeptides in conjunction with the analysis of phosphopeptides.
29741879	6	28	theme	phosphoproteomic	1338:1353	arg1	data					1355:1358	global and phosphoproteomic data	1327:1358	global and phosphoproteomic data generated from 108 human breast cancer tissues that were previously analyzed by Clinical Proteomic Analysis Consortium (CPTAC)	1327:1485	Next, we utilized GPQuest 2.0 for the reanalysis of global and phosphoproteomic data generated from 108 human breast cancer tissues that were previously analyzed by Clinical Proteomic Analysis Consortium (CPTAC).
29741879	8	29	from	glycoproteins	1778:1790	arg1	glycosites					1752:1761	1 731 N-linked glycosites	1737:1761	1 731 N-linked glycosites from 883 human glycoproteins	1737:1790	Together, 11 292 N-linked glycopeptides corresponding to 1 731 N-linked glycosites from 883 human glycoproteins were identified from the two data sets.
29741879	10	30	theme	existing	2155:2162	arg1	sets					2169:2172	already existing data sets	2147:2172	already existing data sets	2147:2172	The reanalysis described herein can be readily applied to identify glycopeptides from already existing data sets, providing insight into many important facets of protein glycosylation in different biological, physiological, and pathological processes.
29741879	9	31	dep	glycopeptides	1878:1890	arg1	enriched					1917:1924	enriched	1917:1924	enriched in IMAC-based phosphopeptide-enriched proteomic data, information which would have remained unknown from the original study otherwise	1917:2058	This analysis revealed an extensive number of glycopeptides hidden in the global and enriched in IMAC-based phosphopeptide-enriched proteomic data, information which would have remained unknown from the original study otherwise.
29741879	9	31	dep	glycopeptides	1878:1890	arg1	hidden					1892:1897	hidden	1892:1897	hidden in the global	1892:1911	This analysis revealed an extensive number of glycopeptides hidden in the global and enriched in IMAC-based phosphopeptide-enriched proteomic data, information which would have remained unknown from the original study otherwise.
29741879	9	32	gly	glycopeptides	1878:1890	arg2	glycopeptides					1878:1890	glycopeptides	1878:1890	glycopeptides hidden in the global and enriched in IMAC-based phosphopeptide-enriched proteomic data, information which would have remained unknown from the original study otherwise	1878:2058	This analysis revealed an extensive number of glycopeptides hidden in the global and enriched in IMAC-based phosphopeptide-enriched proteomic data, information which would have remained unknown from the original study otherwise.
29741879	5	33	gly	glycoproteins	1211:1223	arg1	glycoproteins					1211:1223	617 glycoproteins	1207:1223	617 glycoproteins derived from two breast cancer xenograft tissues	1207:1272	Collectively, combined tandem mass spectrometry (MS/MS) analyses of global proteomic and phosphoproteomic data sets resulted in the identification of 6 724 N-linked glycopeptides from 617 glycoproteins derived from two breast cancer xenograft tissues.
29741879	3	34	from	tissues	578:584	arg1	data					544:547	global proteomic data	527:547	global proteomic data from breast cancer xenograft tissues using recently developed software package GPQuest 2.0	527:638	In this study, we describe the reanalysis of global proteomic data from breast cancer xenograft tissues using recently developed software package GPQuest 2.0, revealing a large number of previously unidentified N-linked glycopeptides.
29741879	3	34	from	tissues	578:584	arg1	reanalysis					513:522	the reanalysis	509:522	the reanalysis of global proteomic data from breast cancer xenograft tissues using recently developed software package GPQuest 2.0	509:638	In this study, we describe the reanalysis of global proteomic data from breast cancer xenograft tissues using recently developed software package GPQuest 2.0, revealing a large number of previously unidentified N-linked glycopeptides.
29741879	5	35	theme	N-linked	1179:1186	arg1	glycopeptides					1188:1200	6 724 N-linked glycopeptides	1173:1200	6 724 N-linked glycopeptides from 617 glycoproteins derived from two breast cancer xenograft tissues	1173:1272	Collectively, combined tandem mass spectrometry (MS/MS) analyses of global proteomic and phosphoproteomic data sets resulted in the identification of 6 724 N-linked glycopeptides from 617 glycoproteins derived from two breast cancer xenograft tissues.
29741879	5	36	from	identification	1155:1168	arg1	glycoproteins					1211:1223	617 glycoproteins	1207:1223	617 glycoproteins derived from two breast cancer xenograft tissues	1207:1272	Collectively, combined tandem mass spectrometry (MS/MS) analyses of global proteomic and phosphoproteomic data sets resulted in the identification of 6 724 N-linked glycopeptides from 617 glycoproteins derived from two breast cancer xenograft tissues.
29741879	1	37	theme	cellular	155:162	arg1	processes					164:172	many cellular processes	150:172	many cellular processes	150:172	Protein glycosylation plays fundamental roles in many cellular processes, and previous reports have shown dysregulation to be associated with several human diseases, including diabetes, cancer, and neurodegenerative disorders.
29741879	9	38	theme	extensive	1858:1866	arg1	number					1868:1873	an extensive number	1855:1873	an extensive number of glycopeptides hidden in the global and enriched in IMAC-based phosphopeptide-enriched proteomic data, information which would have remained unknown from the original study otherwise	1855:2058	This analysis revealed an extensive number of glycopeptides hidden in the global and enriched in IMAC-based phosphopeptide-enriched proteomic data, information which would have remained unknown from the original study otherwise.
29741879	6	39	theme	Proteomic	1449:1457	arg1	CPTAC					1480:1484	CPTAC	1480:1484	CPTAC	1480:1484	Next, we utilized GPQuest 2.0 for the reanalysis of global and phosphoproteomic data generated from 108 human breast cancer tissues that were previously analyzed by Clinical Proteomic Analysis Consortium (CPTAC).
29741879	6	39	theme	Proteomic	1449:1457	arg1	Consortium					1468:1477	Clinical Proteomic Analysis Consortium	1440:1477	Clinical Proteomic Analysis Consortium (CPTAC)	1440:1485	Next, we utilized GPQuest 2.0 for the reanalysis of global and phosphoproteomic data generated from 108 human breast cancer tissues that were previously analyzed by Clinical Proteomic Analysis Consortium (CPTAC).
29741879	7	40	theme	data	1656:1659	arg1	set					1661:1663	phosphoproteomic data set	1639:1663	phosphoproteomic data set	1639:1663	Reanalysis of the CPTAC data set resulted in the identification of 2 683 glycopeptides from the global proteomic data set and 4 554 glycopeptides from phosphoproteomic data set, respectively.
29741879	10	41	from	sets	2169:2172	arg1	glycopeptides					2128:2140	glycopeptides	2128:2140	glycopeptides from already existing data sets	2128:2172	The reanalysis described herein can be readily applied to identify glycopeptides from already existing data sets, providing insight into many important facets of protein glycosylation in different biological, physiological, and pathological processes.
29741879	0	42	theme	Glycopeptides	86:98	arg1	Number					76:81	a Large Number	68:81	a Large Number of Glycopeptides	68:98	Reanalysis of Global Proteomic and Phosphoproteomic Data Identified a Large Number of Glycopeptides.
29741879	8	43	link	N-linked	1743:1750	arg1	glycosites					1752:1761	1 731 N-linked glycosites	1737:1761	1 731 N-linked glycosites from 883 human glycoproteins	1737:1790	Together, 11 292 N-linked glycopeptides corresponding to 1 731 N-linked glycosites from 883 human glycoproteins were identified from the two data sets.
29741879	4	44	theme	phosphopeptides	1006:1020	arg1	analysis					994:1001	the analysis	990:1001	the analysis of phosphopeptides	990:1020	More importantly, we found that using immobilized metal affinity chromatography (IMAC) technology for the enrichment of phosphopeptides had coenriched a substantial number of sialoglycopeptides, allowing for a large-scale analysis of sialoglycopeptides in conjunction with the analysis of phosphopeptides.
29741879	5	45	theme	combined	1037:1044	arg1	spectrometry					1058:1069	combined tandem mass spectrometry	1037:1069	combined tandem mass spectrometry (MS/MS) analyses of global proteomic and phosphoproteomic data sets	1037:1137	Collectively, combined tandem mass spectrometry (MS/MS) analyses of global proteomic and phosphoproteomic data sets resulted in the identification of 6 724 N-linked glycopeptides from 617 glycoproteins derived from two breast cancer xenograft tissues.
29741879	5	45	theme	combined	1037:1044	arg1	MS/MS					1072:1076	MS/MS	1072:1076	MS/MS	1072:1076	Collectively, combined tandem mass spectrometry (MS/MS) analyses of global proteomic and phosphoproteomic data sets resulted in the identification of 6 724 N-linked glycopeptides from 617 glycoproteins derived from two breast cancer xenograft tissues.
29741879	2	46	theme	other	453:457	arg1	modifications					467:479	other protein modifications	453:479	other protein modifications	453:479	Despite the vital role of glycosylation for proper protein function, the analysis of glycoproteins has been lagged behind to other protein modifications.
29741879	4	47	theme	immobilized	755:765	arg1	IMAC					798:801	IMAC	798:801	IMAC	798:801	More importantly, we found that using immobilized metal affinity chromatography (IMAC) technology for the enrichment of phosphopeptides had coenriched a substantial number of sialoglycopeptides, allowing for a large-scale analysis of sialoglycopeptides in conjunction with the analysis of phosphopeptides.
29741879	4	47	theme	immobilized	755:765	arg1	chromatography					782:795	immobilized metal affinity chromatography	755:795	immobilized metal affinity chromatography (IMAC) technology for the enrichment of phosphopeptides	755:851	More importantly, we found that using immobilized metal affinity chromatography (IMAC) technology for the enrichment of phosphopeptides had coenriched a substantial number of sialoglycopeptides, allowing for a large-scale analysis of sialoglycopeptides in conjunction with the analysis of phosphopeptides.
29741879	5	48	theme	breast	1242:1247	arg1	tissues					1266:1272	two breast cancer xenograft tissues	1238:1272	two breast cancer xenograft tissues	1238:1272	Collectively, combined tandem mass spectrometry (MS/MS) analyses of global proteomic and phosphoproteomic data sets resulted in the identification of 6 724 N-linked glycopeptides from 617 glycoproteins derived from two breast cancer xenograft tissues.
29741879	7	49	gly	glycopeptides	1561:1573	arg2	glycopeptides					1561:1573	2 683 glycopeptides	1555:1573	2 683 glycopeptides from the global proteomic data set	1555:1608	Reanalysis of the CPTAC data set resulted in the identification of 2 683 glycopeptides from the global proteomic data set and 4 554 glycopeptides from phosphoproteomic data set, respectively.
29741879	7	49	gly	glycopeptides	1561:1573	arg1	set					1606:1608	the global proteomic data set	1580:1608	the global proteomic data set	1580:1608	Reanalysis of the CPTAC data set resulted in the identification of 2 683 glycopeptides from the global proteomic data set and 4 554 glycopeptides from phosphoproteomic data set, respectively.
29741879	7	49	gly	glycopeptides	1561:1573	arg1	set					1661:1663	phosphoproteomic data set	1639:1663	phosphoproteomic data set	1639:1663	Reanalysis of the CPTAC data set resulted in the identification of 2 683 glycopeptides from the global proteomic data set and 4 554 glycopeptides from phosphoproteomic data set, respectively.
29741879	4	50	theme	affinity	773:780	arg1	IMAC					798:801	IMAC	798:801	IMAC	798:801	More importantly, we found that using immobilized metal affinity chromatography (IMAC) technology for the enrichment of phosphopeptides had coenriched a substantial number of sialoglycopeptides, allowing for a large-scale analysis of sialoglycopeptides in conjunction with the analysis of phosphopeptides.
29741879	4	50	theme	affinity	773:780	arg1	chromatography					782:795	immobilized metal affinity chromatography	755:795	immobilized metal affinity chromatography (IMAC) technology for the enrichment of phosphopeptides	755:851	More importantly, we found that using immobilized metal affinity chromatography (IMAC) technology for the enrichment of phosphopeptides had coenriched a substantial number of sialoglycopeptides, allowing for a large-scale analysis of sialoglycopeptides in conjunction with the analysis of phosphopeptides.
29741879	0	51	theme	Global	14:19	arg1	Data					52:55	Global Proteomic and Phosphoproteomic Data	14:55	Global Proteomic and Phosphoproteomic Data	14:55	Reanalysis of Global Proteomic and Phosphoproteomic Data Identified a Large Number of Glycopeptides.
29741879	5	52	theme	xenograft	1256:1264	arg1	tissues					1266:1272	two breast cancer xenograft tissues	1238:1272	two breast cancer xenograft tissues	1238:1272	Collectively, combined tandem mass spectrometry (MS/MS) analyses of global proteomic and phosphoproteomic data sets resulted in the identification of 6 724 N-linked glycopeptides from 617 glycoproteins derived from two breast cancer xenograft tissues.
29741879	5	53	link	N-linked	1179:1186	arg1	glycopeptides					1188:1200	6 724 N-linked glycopeptides	1173:1200	6 724 N-linked glycopeptides from 617 glycoproteins derived from two breast cancer xenograft tissues	1173:1272	Collectively, combined tandem mass spectrometry (MS/MS) analyses of global proteomic and phosphoproteomic data sets resulted in the identification of 6 724 N-linked glycopeptides from 617 glycoproteins derived from two breast cancer xenograft tissues.
29741879	8	54	gly	glycopeptides	1706:1718	arg2	glycopeptides					1706:1718	11 292 N-linked glycopeptides	1690:1718	11 292 N-linked glycopeptides corresponding to 1 731 N-linked glycosites from 883 human glycoproteins	1690:1790	Together, 11 292 N-linked glycopeptides corresponding to 1 731 N-linked glycosites from 883 human glycoproteins were identified from the two data sets.
29741879	4	55	theme	phosphopeptides	837:851	arg1	enrichment					823:832	the enrichment	819:832	the enrichment of phosphopeptides	819:851	More importantly, we found that using immobilized metal affinity chromatography (IMAC) technology for the enrichment of phosphopeptides had coenriched a substantial number of sialoglycopeptides, allowing for a large-scale analysis of sialoglycopeptides in conjunction with the analysis of phosphopeptides.
29741879	2	56	theme	glycoproteins	413:425	arg1	analysis					401:408	the analysis	397:408	the analysis of glycoproteins	397:425	Despite the vital role of glycosylation for proper protein function, the analysis of glycoproteins has been lagged behind to other protein modifications.
29741879	1	57	theme	neurodegenerative	299:315	arg1	disorders					317:325	neurodegenerative disorders	299:325	neurodegenerative disorders	299:325	Protein glycosylation plays fundamental roles in many cellular processes, and previous reports have shown dysregulation to be associated with several human diseases, including diabetes, cancer, and neurodegenerative disorders.
29741879	7	58	theme	data	1601:1604	arg1	set					1606:1608	the global proteomic data set	1580:1608	the global proteomic data set	1580:1608	Reanalysis of the CPTAC data set resulted in the identification of 2 683 glycopeptides from the global proteomic data set and 4 554 glycopeptides from phosphoproteomic data set, respectively.
29741879	9	59	theme	phosphopeptide-enriched	1940:1962	arg1	information					1980:1990	information	1980:1990	information which would have remained unknown from the original study otherwise	1980:2058	This analysis revealed an extensive number of glycopeptides hidden in the global and enriched in IMAC-based phosphopeptide-enriched proteomic data, information which would have remained unknown from the original study otherwise.
29741879	9	59	theme	phosphopeptide-enriched	1940:1962	arg1	data					1974:1977	IMAC-based phosphopeptide-enriched proteomic data	1929:1977	IMAC-based phosphopeptide-enriched proteomic data	1929:1977	This analysis revealed an extensive number of glycopeptides hidden in the global and enriched in IMAC-based phosphopeptide-enriched proteomic data, information which would have remained unknown from the original study otherwise.
29741879	0	60	theme	Data	52:55	arg1	Reanalysis					0:9	Reanalysis	0:9	Reanalysis of Global Proteomic and Phosphoproteomic Data	0:55	Reanalysis of Global Proteomic and Phosphoproteomic Data Identified a Large Number of Glycopeptides.
29741879	7	61	theme	global	1584:1589	arg1	set					1606:1608	the global proteomic data set	1580:1608	the global proteomic data set	1580:1608	Reanalysis of the CPTAC data set resulted in the identification of 2 683 glycopeptides from the global proteomic data set and 4 554 glycopeptides from phosphoproteomic data set, respectively.
29741879	10	62	theme	important	2203:2211	arg1	facets					2213:2218	many important facets	2198:2218	many important facets of protein glycosylation	2198:2243	The reanalysis described herein can be readily applied to identify glycopeptides from already existing data sets, providing insight into many important facets of protein glycosylation in different biological, physiological, and pathological processes.
29741879	3	63	gly	glycopeptides	702:714	arg2	glycopeptides					702:714	previously unidentified N-linked glycopeptides	669:714	previously unidentified N-linked glycopeptides	669:714	In this study, we describe the reanalysis of global proteomic data from breast cancer xenograft tissues using recently developed software package GPQuest 2.0, revealing a large number of previously unidentified N-linked glycopeptides.
29741879	3	64	theme	N-linked	693:700	arg1	glycopeptides					702:714	previously unidentified N-linked glycopeptides	669:714	previously unidentified N-linked glycopeptides	669:714	In this study, we describe the reanalysis of global proteomic data from breast cancer xenograft tissues using recently developed software package GPQuest 2.0, revealing a large number of previously unidentified N-linked glycopeptides.
29741879	2	65	theme	vital	340:344	arg1	role					346:349	the vital role	336:349	the vital role of glycosylation for proper protein function	336:394	Despite the vital role of glycosylation for proper protein function, the analysis of glycoproteins has been lagged behind to other protein modifications.
29741879	8	66	theme	N-linked	1743:1750	arg1	glycosites					1752:1761	1 731 N-linked glycosites	1737:1761	1 731 N-linked glycosites from 883 human glycoproteins	1737:1790	Together, 11 292 N-linked glycopeptides corresponding to 1 731 N-linked glycosites from 883 human glycoproteins were identified from the two data sets.
29741879	5	67	theme	mass	1053:1056	arg1	spectrometry					1058:1069	combined tandem mass spectrometry	1037:1069	combined tandem mass spectrometry (MS/MS) analyses of global proteomic and phosphoproteomic data sets	1037:1137	Collectively, combined tandem mass spectrometry (MS/MS) analyses of global proteomic and phosphoproteomic data sets resulted in the identification of 6 724 N-linked glycopeptides from 617 glycoproteins derived from two breast cancer xenograft tissues.
29741879	5	67	theme	mass	1053:1056	arg1	MS/MS					1072:1076	MS/MS	1072:1076	MS/MS	1072:1076	Collectively, combined tandem mass spectrometry (MS/MS) analyses of global proteomic and phosphoproteomic data sets resulted in the identification of 6 724 N-linked glycopeptides from 617 glycoproteins derived from two breast cancer xenograft tissues.
29741879	10	68	theme	different	2248:2256	arg1	processes					2302:2310	different biological, physiological, and pathological processes	2248:2310	different biological, physiological, and pathological processes	2248:2310	The reanalysis described herein can be readily applied to identify glycopeptides from already existing data sets, providing insight into many important facets of protein glycosylation in different biological, physiological, and pathological processes.
29741879	2	69	theme	protein	379:385	arg1	function					387:394	proper protein function	372:394	proper protein function	372:394	Despite the vital role of glycosylation for proper protein function, the analysis of glycoproteins has been lagged behind to other protein modifications.
29741879	1	70	theme	several	243:249	arg1	disorders					317:325	neurodegenerative disorders	299:325	neurodegenerative disorders	299:325	Protein glycosylation plays fundamental roles in many cellular processes, and previous reports have shown dysregulation to be associated with several human diseases, including diabetes, cancer, and neurodegenerative disorders.
29741879	1	70	theme	several	243:249	arg1	cancer					287:292	cancer	287:292	cancer	287:292	Protein glycosylation plays fundamental roles in many cellular processes, and previous reports have shown dysregulation to be associated with several human diseases, including diabetes, cancer, and neurodegenerative disorders.
29741879	1	70	theme	several	243:249	arg1	diseases					257:264	several human diseases	243:264	several human diseases	243:264	Protein glycosylation plays fundamental roles in many cellular processes, and previous reports have shown dysregulation to be associated with several human diseases, including diabetes, cancer, and neurodegenerative disorders.
29741879	1	70	theme	several	243:249	arg1	diabetes					277:284	diabetes	277:284	diabetes	277:284	Protein glycosylation plays fundamental roles in many cellular processes, and previous reports have shown dysregulation to be associated with several human diseases, including diabetes, cancer, and neurodegenerative disorders.
29741879	10	71	theme	glycosylation	2231:2243	arg1	facets					2213:2218	many important facets	2198:2218	many important facets of protein glycosylation	2198:2243	The reanalysis described herein can be readily applied to identify glycopeptides from already existing data sets, providing insight into many important facets of protein glycosylation in different biological, physiological, and pathological processes.
29741879	8	72	theme	human	1772:1776	arg1	glycoproteins					1778:1790	883 human glycoproteins	1768:1790	883 human glycoproteins	1768:1790	Together, 11 292 N-linked glycopeptides corresponding to 1 731 N-linked glycosites from 883 human glycoproteins were identified from the two data sets.
29741879	4	73	theme	sialoglycopeptides	892:909	arg1	number					882:887	a substantial number	868:887	a substantial number	868:887	More importantly, we found that using immobilized metal affinity chromatography (IMAC) technology for the enrichment of phosphopeptides had coenriched a substantial number of sialoglycopeptides, allowing for a large-scale analysis of sialoglycopeptides in conjunction with the analysis of phosphopeptides.
29741879	5	74	from	glycoproteins	1211:1223	arg1	glycopeptides					1188:1200	6 724 N-linked glycopeptides	1173:1200	6 724 N-linked glycopeptides from 617 glycoproteins derived from two breast cancer xenograft tissues	1173:1272	Collectively, combined tandem mass spectrometry (MS/MS) analyses of global proteomic and phosphoproteomic data sets resulted in the identification of 6 724 N-linked glycopeptides from 617 glycoproteins derived from two breast cancer xenograft tissues.
29741879	5	74	from	glycoproteins	1211:1223	arg1	identification					1155:1168	the identification	1151:1168	the identification of 6 724 N-linked glycopeptides from 617 glycoproteins derived from two breast cancer xenograft tissues	1151:1272	Collectively, combined tandem mass spectrometry (MS/MS) analyses of global proteomic and phosphoproteomic data sets resulted in the identification of 6 724 N-linked glycopeptides from 617 glycoproteins derived from two breast cancer xenograft tissues.
29741879	3	75	theme	data	544:547	arg1	reanalysis					513:522	the reanalysis	509:522	the reanalysis of global proteomic data from breast cancer xenograft tissues using recently developed software package GPQuest 2.0	509:638	In this study, we describe the reanalysis of global proteomic data from breast cancer xenograft tissues using recently developed software package GPQuest 2.0, revealing a large number of previously unidentified N-linked glycopeptides.
29741879	7	76	from	set	1606:1608	arg1	glycopeptides					1620:1632	4 554 glycopeptides	1614:1632	4 554 glycopeptides from phosphoproteomic data set	1614:1663	Reanalysis of the CPTAC data set resulted in the identification of 2 683 glycopeptides from the global proteomic data set and 4 554 glycopeptides from phosphoproteomic data set, respectively.
29741879	7	76	from	set	1606:1608	arg1	identification					1537:1550	the identification	1533:1550	the identification of 2 683 glycopeptides from the global proteomic data set and 4 554 glycopeptides from phosphoproteomic data set	1533:1663	Reanalysis of the CPTAC data set resulted in the identification of 2 683 glycopeptides from the global proteomic data set and 4 554 glycopeptides from phosphoproteomic data set, respectively.
29741879	7	76	from	set	1606:1608	arg1	glycopeptides					1561:1573	2 683 glycopeptides	1555:1573	2 683 glycopeptides from the global proteomic data set	1555:1608	Reanalysis of the CPTAC data set resulted in the identification of 2 683 glycopeptides from the global proteomic data set and 4 554 glycopeptides from phosphoproteomic data set, respectively.
29741879	7	77	theme	data	1512:1515	arg1	set					1517:1519	the CPTAC data set	1502:1519	the CPTAC data set	1502:1519	Reanalysis of the CPTAC data set resulted in the identification of 2 683 glycopeptides from the global proteomic data set and 4 554 glycopeptides from phosphoproteomic data set, respectively.
29741879	5	78	gly	glycopeptides	1188:1200	arg2	glycopeptides					1188:1200	6 724 N-linked glycopeptides	1173:1200	6 724 N-linked glycopeptides from 617 glycoproteins derived from two breast cancer xenograft tissues	1173:1272	Collectively, combined tandem mass spectrometry (MS/MS) analyses of global proteomic and phosphoproteomic data sets resulted in the identification of 6 724 N-linked glycopeptides from 617 glycoproteins derived from two breast cancer xenograft tissues.
29741879	5	78	gly	glycopeptides	1188:1200	arg1	glycoproteins					1211:1223	617 glycoproteins	1207:1223	617 glycoproteins derived from two breast cancer xenograft tissues	1207:1272	Collectively, combined tandem mass spectrometry (MS/MS) analyses of global proteomic and phosphoproteomic data sets resulted in the identification of 6 724 N-linked glycopeptides from 617 glycoproteins derived from two breast cancer xenograft tissues.
29741879	6	79	theme	global	1327:1332	arg1	data					1355:1358	global and phosphoproteomic data	1327:1358	global and phosphoproteomic data generated from 108 human breast cancer tissues that were previously analyzed by Clinical Proteomic Analysis Consortium (CPTAC)	1327:1485	Next, we utilized GPQuest 2.0 for the reanalysis of global and phosphoproteomic data generated from 108 human breast cancer tissues that were previously analyzed by Clinical Proteomic Analysis Consortium (CPTAC).
29741879	3	80	theme	global	527:532	arg1	data					544:547	global proteomic data	527:547	global proteomic data from breast cancer xenograft tissues using recently developed software package GPQuest 2.0	527:638	In this study, we describe the reanalysis of global proteomic data from breast cancer xenograft tissues using recently developed software package GPQuest 2.0, revealing a large number of previously unidentified N-linked glycopeptides.
29741879	4	81	gly	sialoglycopeptides	892:909	arg2	sialoglycopeptides					892:909	sialoglycopeptides	892:909	sialoglycopeptides	892:909	More importantly, we found that using immobilized metal affinity chromatography (IMAC) technology for the enrichment of phosphopeptides had coenriched a substantial number of sialoglycopeptides, allowing for a large-scale analysis of sialoglycopeptides in conjunction with the analysis of phosphopeptides.
29741879	9	82	theme	proteomic	1964:1972	arg1	information					1980:1990	information	1980:1990	information which would have remained unknown from the original study otherwise	1980:2058	This analysis revealed an extensive number of glycopeptides hidden in the global and enriched in IMAC-based phosphopeptide-enriched proteomic data, information which would have remained unknown from the original study otherwise.
29741879	9	82	theme	proteomic	1964:1972	arg1	data					1974:1977	IMAC-based phosphopeptide-enriched proteomic data	1929:1977	IMAC-based phosphopeptide-enriched proteomic data	1929:1977	This analysis revealed an extensive number of glycopeptides hidden in the global and enriched in IMAC-based phosphopeptide-enriched proteomic data, information which would have remained unknown from the original study otherwise.
29741879	7	83	theme	set	1517:1519	arg1	Reanalysis					1488:1497	Reanalysis	1488:1497	Reanalysis of the CPTAC data set	1488:1519	Reanalysis of the CPTAC data set resulted in the identification of 2 683 glycopeptides from the global proteomic data set and 4 554 glycopeptides from phosphoproteomic data set, respectively.
29741879	8	84	gly	glycoproteins	1778:1790	arg1	glycoproteins					1778:1790	883 human glycoproteins	1768:1790	883 human glycoproteins	1768:1790	Together, 11 292 N-linked glycopeptides corresponding to 1 731 N-linked glycosites from 883 human glycoproteins were identified from the two data sets.
29741879	9	85	theme	original	2035:2042	arg1	study					2044:2048	the original study	2031:2048	the original study	2031:2048	This analysis revealed an extensive number of glycopeptides hidden in the global and enriched in IMAC-based phosphopeptide-enriched proteomic data, information which would have remained unknown from the original study otherwise.
29741879	3	86	theme	xenograft	568:576	arg1	tissues					578:584	breast cancer xenograft tissues	554:584	breast cancer xenograft tissues using recently developed software package GPQuest 2.0	554:638	In this study, we describe the reanalysis of global proteomic data from breast cancer xenograft tissues using recently developed software package GPQuest 2.0, revealing a large number of previously unidentified N-linked glycopeptides.
29741879	10	87	theme	pathological	2289:2300	arg1	processes					2302:2310	different biological, physiological, and pathological processes	2248:2310	different biological, physiological, and pathological processes	2248:2310	The reanalysis described herein can be readily applied to identify glycopeptides from already existing data sets, providing insight into many important facets of protein glycosylation in different biological, physiological, and pathological processes.
29741879	3	88	theme	breast	554:559	arg1	tissues					578:584	breast cancer xenograft tissues	554:584	breast cancer xenograft tissues using recently developed software package GPQuest 2.0	554:638	In this study, we describe the reanalysis of global proteomic data from breast cancer xenograft tissues using recently developed software package GPQuest 2.0, revealing a large number of previously unidentified N-linked glycopeptides.
29741879	4	89	theme	sialoglycopeptides	951:968	arg1	analysis					939:946	a large-scale analysis	925:946	a large-scale analysis of sialoglycopeptides in conjunction with the analysis of phosphopeptides	925:1020	More importantly, we found that using immobilized metal affinity chromatography (IMAC) technology for the enrichment of phosphopeptides had coenriched a substantial number of sialoglycopeptides, allowing for a large-scale analysis of sialoglycopeptides in conjunction with the analysis of phosphopeptides.
29741879	5	90	theme	sets	1134:1137	arg1	analyses					1079:1086	combined tandem mass spectrometry (MS/MS) analyses	1037:1086	combined tandem mass spectrometry (MS/MS) analyses of global proteomic and phosphoproteomic data sets	1037:1137	Collectively, combined tandem mass spectrometry (MS/MS) analyses of global proteomic and phosphoproteomic data sets resulted in the identification of 6 724 N-linked glycopeptides from 617 glycoproteins derived from two breast cancer xenograft tissues.
29741879	3	91	theme	developed	601:609	arg1	package					620:626	recently developed software package GPQuest 2.0	592:638	recently developed software package GPQuest 2.0	592:638	In this study, we describe the reanalysis of global proteomic data from breast cancer xenograft tissues using recently developed software package GPQuest 2.0, revealing a large number of previously unidentified N-linked glycopeptides.
29741879	6	92	theme	breast	1385:1390	arg1	tissues					1399:1405	108 human breast cancer tissues	1375:1405	108 human breast cancer tissues that were previously analyzed by Clinical Proteomic Analysis Consortium (CPTAC)	1375:1485	Next, we utilized GPQuest 2.0 for the reanalysis of global and phosphoproteomic data generated from 108 human breast cancer tissues that were previously analyzed by Clinical Proteomic Analysis Consortium (CPTAC).
29741879	4	93	from	sialoglycopeptides	951:968	arg1	conjunction					973:983	conjunction	973:983	conjunction with the analysis of phosphopeptides	973:1020	More importantly, we found that using immobilized metal affinity chromatography (IMAC) technology for the enrichment of phosphopeptides had coenriched a substantial number of sialoglycopeptides, allowing for a large-scale analysis of sialoglycopeptides in conjunction with the analysis of phosphopeptides.
29741879	4	94	gly	sialoglycopeptides	951:968	arg2	sialoglycopeptides					951:968	sialoglycopeptides	951:968	sialoglycopeptides in conjunction with the analysis of phosphopeptides	951:1020	More importantly, we found that using immobilized metal affinity chromatography (IMAC) technology for the enrichment of phosphopeptides had coenriched a substantial number of sialoglycopeptides, allowing for a large-scale analysis of sialoglycopeptides in conjunction with the analysis of phosphopeptides.
29741879	5	95	theme	proteomic	1098:1106	arg1	sets					1134:1137	global proteomic and phosphoproteomic data sets	1091:1137	global proteomic and phosphoproteomic data sets	1091:1137	Collectively, combined tandem mass spectrometry (MS/MS) analyses of global proteomic and phosphoproteomic data sets resulted in the identification of 6 724 N-linked glycopeptides from 617 glycoproteins derived from two breast cancer xenograft tissues.
29741879	9	96	from	study	2044:2048	arg1	unknown					2018:2024	unknown	2018:2024	unknown	2018:2024	This analysis revealed an extensive number of glycopeptides hidden in the global and enriched in IMAC-based phosphopeptide-enriched proteomic data, information which would have remained unknown from the original study otherwise.
29741879	0	97	theme	Large	70:74	arg1	Number					76:81	a Large Number	68:81	a Large Number of Glycopeptides	68:98	Reanalysis of Global Proteomic and Phosphoproteomic Data Identified a Large Number of Glycopeptides.
29741879	5	98	theme	phosphoproteomic	1112:1127	arg1	sets					1134:1137	global proteomic and phosphoproteomic data sets	1091:1137	global proteomic and phosphoproteomic data sets	1091:1137	Collectively, combined tandem mass spectrometry (MS/MS) analyses of global proteomic and phosphoproteomic data sets resulted in the identification of 6 724 N-linked glycopeptides from 617 glycoproteins derived from two breast cancer xenograft tissues.
29741879	1	99	theme	many	150:153	arg1	processes					164:172	many cellular processes	150:172	many cellular processes	150:172	Protein glycosylation plays fundamental roles in many cellular processes, and previous reports have shown dysregulation to be associated with several human diseases, including diabetes, cancer, and neurodegenerative disorders.
29741879	6	100	theme	Analysis	1459:1466	arg1	CPTAC					1480:1484	CPTAC	1480:1484	CPTAC	1480:1484	Next, we utilized GPQuest 2.0 for the reanalysis of global and phosphoproteomic data generated from 108 human breast cancer tissues that were previously analyzed by Clinical Proteomic Analysis Consortium (CPTAC).
29741879	6	100	theme	Analysis	1459:1466	arg1	Consortium					1468:1477	Clinical Proteomic Analysis Consortium	1440:1477	Clinical Proteomic Analysis Consortium (CPTAC)	1440:1485	Next, we utilized GPQuest 2.0 for the reanalysis of global and phosphoproteomic data generated from 108 human breast cancer tissues that were previously analyzed by Clinical Proteomic Analysis Consortium (CPTAC).
29741879	5	101	theme	glycopeptides	1188:1200	arg1	identification					1155:1168	the identification	1151:1168	the identification of 6 724 N-linked glycopeptides from 617 glycoproteins derived from two breast cancer xenograft tissues	1151:1272	Collectively, combined tandem mass spectrometry (MS/MS) analyses of global proteomic and phosphoproteomic data sets resulted in the identification of 6 724 N-linked glycopeptides from 617 glycoproteins derived from two breast cancer xenograft tissues.
29741879	3	102	theme	GPQuest	628:634	arg1	package					620:626	recently developed software package GPQuest 2.0	592:638	recently developed software package GPQuest 2.0	592:638	In this study, we describe the reanalysis of global proteomic data from breast cancer xenograft tissues using recently developed software package GPQuest 2.0, revealing a large number of previously unidentified N-linked glycopeptides.
29741879	7	103	theme	phosphoproteomic	1639:1654	arg1	set					1661:1663	phosphoproteomic data set	1639:1663	phosphoproteomic data set	1639:1663	Reanalysis of the CPTAC data set resulted in the identification of 2 683 glycopeptides from the global proteomic data set and 4 554 glycopeptides from phosphoproteomic data set, respectively.
29741879	6	104	theme	Clinical	1440:1447	arg1	CPTAC					1480:1484	CPTAC	1480:1484	CPTAC	1480:1484	Next, we utilized GPQuest 2.0 for the reanalysis of global and phosphoproteomic data generated from 108 human breast cancer tissues that were previously analyzed by Clinical Proteomic Analysis Consortium (CPTAC).
29741879	6	104	theme	Clinical	1440:1447	arg1	Consortium					1468:1477	Clinical Proteomic Analysis Consortium	1440:1477	Clinical Proteomic Analysis Consortium (CPTAC)	1440:1485	Next, we utilized GPQuest 2.0 for the reanalysis of global and phosphoproteomic data generated from 108 human breast cancer tissues that were previously analyzed by Clinical Proteomic Analysis Consortium (CPTAC).
29741879	5	105	theme	617	1207:1209	arg1	glycoproteins					1211:1223	617 glycoproteins	1207:1223	617 glycoproteins derived from two breast cancer xenograft tissues	1207:1272	Collectively, combined tandem mass spectrometry (MS/MS) analyses of global proteomic and phosphoproteomic data sets resulted in the identification of 6 724 N-linked glycopeptides from 617 glycoproteins derived from two breast cancer xenograft tissues.
29741879	7	106	theme	glycopeptides	1620:1632	arg1	identification					1537:1550	the identification	1533:1550	the identification of 2 683 glycopeptides from the global proteomic data set and 4 554 glycopeptides from phosphoproteomic data set	1533:1663	Reanalysis of the CPTAC data set resulted in the identification of 2 683 glycopeptides from the global proteomic data set and 4 554 glycopeptides from phosphoproteomic data set, respectively.
29741879	9	107	theme	glycopeptides	1878:1890	arg1	number					1868:1873	an extensive number	1855:1873	an extensive number of glycopeptides hidden in the global and enriched in IMAC-based phosphopeptide-enriched proteomic data, information which would have remained unknown from the original study otherwise	1855:2058	This analysis revealed an extensive number of glycopeptides hidden in the global and enriched in IMAC-based phosphopeptide-enriched proteomic data, information which would have remained unknown from the original study otherwise.
29741879	2	108	gly	glycoproteins	413:425	arg1	glycoproteins					413:425	glycoproteins	413:425	glycoproteins	413:425	Despite the vital role of glycosylation for proper protein function, the analysis of glycoproteins has been lagged behind to other protein modifications.
29741879	4	109	theme	chromatography	782:795	arg1	technology					804:813	immobilized metal affinity chromatography (IMAC) technology	755:813	immobilized metal affinity chromatography (IMAC) technology for the enrichment of phosphopeptides	755:851	More importantly, we found that using immobilized metal affinity chromatography (IMAC) technology for the enrichment of phosphopeptides had coenriched a substantial number of sialoglycopeptides, allowing for a large-scale analysis of sialoglycopeptides in conjunction with the analysis of phosphopeptides.
29741879	10	110	theme	data	2164:2167	arg1	sets					2169:2172	already existing data sets	2147:2172	already existing data sets	2147:2172	The reanalysis described herein can be readily applied to identify glycopeptides from already existing data sets, providing insight into many important facets of protein glycosylation in different biological, physiological, and pathological processes.
29741879	4	111	with	conjunction	973:983	arg1	analysis					994:1001	the analysis	990:1001	the analysis of phosphopeptides	990:1020	More importantly, we found that using immobilized metal affinity chromatography (IMAC) technology for the enrichment of phosphopeptides had coenriched a substantial number of sialoglycopeptides, allowing for a large-scale analysis of sialoglycopeptides in conjunction with the analysis of phosphopeptides.
29741879	4	112	from	conjunction	973:983	arg1	analysis					939:946	a large-scale analysis	925:946	a large-scale analysis of sialoglycopeptides in conjunction with the analysis of phosphopeptides	925:1020	More importantly, we found that using immobilized metal affinity chromatography (IMAC) technology for the enrichment of phosphopeptides had coenriched a substantial number of sialoglycopeptides, allowing for a large-scale analysis of sialoglycopeptides in conjunction with the analysis of phosphopeptides.
29741879	0	113	theme	Proteomic	21:29	arg1	Data					52:55	Global Proteomic and Phosphoproteomic Data	14:55	Global Proteomic and Phosphoproteomic Data	14:55	Reanalysis of Global Proteomic and Phosphoproteomic Data Identified a Large Number of Glycopeptides.
29741879	5	114	theme	cancer	1249:1254	arg1	tissues					1266:1272	two breast cancer xenograft tissues	1238:1272	two breast cancer xenograft tissues	1238:1272	Collectively, combined tandem mass spectrometry (MS/MS) analyses of global proteomic and phosphoproteomic data sets resulted in the identification of 6 724 N-linked glycopeptides from 617 glycoproteins derived from two breast cancer xenograft tissues.
29741879	1	115	theme	Protein	101:107	arg1	glycosylation					109:121	Protein glycosylation	101:121	Protein glycosylation	101:121	Protein glycosylation plays fundamental roles in many cellular processes, and previous reports have shown dysregulation to be associated with several human diseases, including diabetes, cancer, and neurodegenerative disorders.
29741879	3	116	theme	large	653:657	arg1	number					659:664	a large number	651:664	a large number of previously unidentified N-linked glycopeptides	651:714	In this study, we describe the reanalysis of global proteomic data from breast cancer xenograft tissues using recently developed software package GPQuest 2.0, revealing a large number of previously unidentified N-linked glycopeptides.
29741879	4	117	theme	metal	767:771	arg1	IMAC					798:801	IMAC	798:801	IMAC	798:801	More importantly, we found that using immobilized metal affinity chromatography (IMAC) technology for the enrichment of phosphopeptides had coenriched a substantial number of sialoglycopeptides, allowing for a large-scale analysis of sialoglycopeptides in conjunction with the analysis of phosphopeptides.
29741879	4	117	theme	metal	767:771	arg1	chromatography					782:795	immobilized metal affinity chromatography	755:795	immobilized metal affinity chromatography (IMAC) technology for the enrichment of phosphopeptides	755:851	More importantly, we found that using immobilized metal affinity chromatography (IMAC) technology for the enrichment of phosphopeptides had coenriched a substantial number of sialoglycopeptides, allowing for a large-scale analysis of sialoglycopeptides in conjunction with the analysis of phosphopeptides.
29741879	0	118	theme	Phosphoproteomic	35:50	arg1	Data					52:55	Global Proteomic and Phosphoproteomic Data	14:55	Global Proteomic and Phosphoproteomic Data	14:55	Reanalysis of Global Proteomic and Phosphoproteomic Data Identified a Large Number of Glycopeptides.
29741879	9	119	theme	IMAC-based	1929:1938	arg1	information					1980:1990	information	1980:1990	information which would have remained unknown from the original study otherwise	1980:2058	This analysis revealed an extensive number of glycopeptides hidden in the global and enriched in IMAC-based phosphopeptide-enriched proteomic data, information which would have remained unknown from the original study otherwise.
29741879	9	119	theme	IMAC-based	1929:1938	arg1	data					1974:1977	IMAC-based phosphopeptide-enriched proteomic data	1929:1977	IMAC-based phosphopeptide-enriched proteomic data	1929:1977	This analysis revealed an extensive number of glycopeptides hidden in the global and enriched in IMAC-based phosphopeptide-enriched proteomic data, information which would have remained unknown from the original study otherwise.
29741879	7	120	from	set	1661:1663	arg1	glycopeptides					1620:1632	4 554 glycopeptides	1614:1632	4 554 glycopeptides from phosphoproteomic data set	1614:1663	Reanalysis of the CPTAC data set resulted in the identification of 2 683 glycopeptides from the global proteomic data set and 4 554 glycopeptides from phosphoproteomic data set, respectively.
29741879	7	120	from	set	1661:1663	arg1	identification					1537:1550	the identification	1533:1550	the identification of 2 683 glycopeptides from the global proteomic data set and 4 554 glycopeptides from phosphoproteomic data set	1533:1663	Reanalysis of the CPTAC data set resulted in the identification of 2 683 glycopeptides from the global proteomic data set and 4 554 glycopeptides from phosphoproteomic data set, respectively.
29741879	7	120	from	set	1661:1663	arg1	glycopeptides					1561:1573	2 683 glycopeptides	1555:1573	2 683 glycopeptides from the global proteomic data set	1555:1608	Reanalysis of the CPTAC data set resulted in the identification of 2 683 glycopeptides from the global proteomic data set and 4 554 glycopeptides from phosphoproteomic data set, respectively.
29741879	2	121	theme	protein	459:465	arg1	modifications					467:479	other protein modifications	453:479	other protein modifications	453:479	Despite the vital role of glycosylation for proper protein function, the analysis of glycoproteins has been lagged behind to other protein modifications.
29741879	3	122	theme	unidentified	680:691	arg1	glycopeptides					702:714	previously unidentified N-linked glycopeptides	669:714	previously unidentified N-linked glycopeptides	669:714	In this study, we describe the reanalysis of global proteomic data from breast cancer xenograft tissues using recently developed software package GPQuest 2.0, revealing a large number of previously unidentified N-linked glycopeptides.
29741879	7	123	theme	proteomic	1591:1599	arg1	set					1606:1608	the global proteomic data set	1580:1608	the global proteomic data set	1580:1608	Reanalysis of the CPTAC data set resulted in the identification of 2 683 glycopeptides from the global proteomic data set and 4 554 glycopeptides from phosphoproteomic data set, respectively.
29741879	5	124	theme	tandem	1046:1051	arg1	spectrometry					1058:1069	combined tandem mass spectrometry	1037:1069	combined tandem mass spectrometry (MS/MS) analyses of global proteomic and phosphoproteomic data sets	1037:1137	Collectively, combined tandem mass spectrometry (MS/MS) analyses of global proteomic and phosphoproteomic data sets resulted in the identification of 6 724 N-linked glycopeptides from 617 glycoproteins derived from two breast cancer xenograft tissues.
29741879	5	124	theme	tandem	1046:1051	arg1	MS/MS					1072:1076	MS/MS	1072:1076	MS/MS	1072:1076	Collectively, combined tandem mass spectrometry (MS/MS) analyses of global proteomic and phosphoproteomic data sets resulted in the identification of 6 724 N-linked glycopeptides from 617 glycoproteins derived from two breast cancer xenograft tissues.
29741879	10	125	theme	many	2198:2201	arg1	facets					2213:2218	many important facets	2198:2218	many important facets of protein glycosylation	2198:2243	The reanalysis described herein can be readily applied to identify glycopeptides from already existing data sets, providing insight into many important facets of protein glycosylation in different biological, physiological, and pathological processes.
29741879	5	126	theme	spectrometry	1058:1069	arg1	analyses					1079:1086	combined tandem mass spectrometry (MS/MS) analyses	1037:1086	combined tandem mass spectrometry (MS/MS) analyses of global proteomic and phosphoproteomic data sets	1037:1137	Collectively, combined tandem mass spectrometry (MS/MS) analyses of global proteomic and phosphoproteomic data sets resulted in the identification of 6 724 N-linked glycopeptides from 617 glycoproteins derived from two breast cancer xenograft tissues.
29741879	3	127	link	N-linked	693:700	arg1	glycopeptides					702:714	previously unidentified N-linked glycopeptides	669:714	previously unidentified N-linked glycopeptides	669:714	In this study, we describe the reanalysis of global proteomic data from breast cancer xenograft tissues using recently developed software package GPQuest 2.0, revealing a large number of previously unidentified N-linked glycopeptides.
29741879	8	128	theme	N-linked	1697:1704	arg1	glycopeptides					1706:1718	11 292 N-linked glycopeptides	1690:1718	11 292 N-linked glycopeptides corresponding to 1 731 N-linked glycosites from 883 human glycoproteins	1690:1790	Together, 11 292 N-linked glycopeptides corresponding to 1 731 N-linked glycosites from 883 human glycoproteins were identified from the two data sets.
29741879	2	129	theme	proper	372:377	arg1	function					387:394	proper protein function	372:394	proper protein function	372:394	Despite the vital role of glycosylation for proper protein function, the analysis of glycoproteins has been lagged behind to other protein modifications.
29741879	6	130	used	utilized	1284:1291	arg2	we					1281:1282	we	1281:1282	we	1281:1282	Next, we utilized GPQuest 2.0 for the reanalysis of global and phosphoproteomic data generated from 108 human breast cancer tissues that were previously analyzed by Clinical Proteomic Analysis Consortium (CPTAC).
29741879	1	131	theme	human	251:255	arg1	disorders					317:325	neurodegenerative disorders	299:325	neurodegenerative disorders	299:325	Protein glycosylation plays fundamental roles in many cellular processes, and previous reports have shown dysregulation to be associated with several human diseases, including diabetes, cancer, and neurodegenerative disorders.
29741879	1	131	theme	human	251:255	arg1	cancer					287:292	cancer	287:292	cancer	287:292	Protein glycosylation plays fundamental roles in many cellular processes, and previous reports have shown dysregulation to be associated with several human diseases, including diabetes, cancer, and neurodegenerative disorders.
29741879	1	131	theme	human	251:255	arg1	diseases					257:264	several human diseases	243:264	several human diseases	243:264	Protein glycosylation plays fundamental roles in many cellular processes, and previous reports have shown dysregulation to be associated with several human diseases, including diabetes, cancer, and neurodegenerative disorders.
29741879	1	131	theme	human	251:255	arg1	diabetes					277:284	diabetes	277:284	diabetes	277:284	Protein glycosylation plays fundamental roles in many cellular processes, and previous reports have shown dysregulation to be associated with several human diseases, including diabetes, cancer, and neurodegenerative disorders.
31132195	11	0	theme	clinical	1463:1470	arg1	feature					1472:1478	a key clinical feature	1457:1478	a key clinical feature in patients with CDG	1457:1499	CONCLUSIONS Hyperkinetic movement disorder is a key clinical feature in patients with CDG.
31132195	11	0	theme	clinical	1463:1470	arg1	disorder					1445:1452	Hyperkinetic movement disorder	1423:1452	Hyperkinetic movement disorder	1423:1452	CONCLUSIONS Hyperkinetic movement disorder is a key clinical feature in patients with CDG.
31132195	0	1	from	disorders	22:30	arg1	disorders					46:54	congenital disorders	35:54	congenital disorders of glycosylation	35:71	Hyperkinetic movement disorders in congenital disorders of glycosylation.
31132195	7	2	located	observed	938:945	arg2	movements					913:921	Involuntary movements	901:921	Involuntary movements	901:921	Involuntary movements were generally observed early in childhood, maintaining a clinical stability over time.
31132195	7	2	located	observed	938:945	arg1	childhood					956:964	childhood	956:964	childhood	956:964	Involuntary movements were generally observed early in childhood, maintaining a clinical stability over time.
31132195	11	3	theme	movement	1436:1443	arg1	feature					1472:1478	a key clinical feature	1457:1478	a key clinical feature in patients with CDG	1457:1499	CONCLUSIONS Hyperkinetic movement disorder is a key clinical feature in patients with CDG.
31132195	11	3	theme	movement	1436:1443	arg1	disorder					1445:1452	Hyperkinetic movement disorder	1423:1452	Hyperkinetic movement disorder	1423:1452	CONCLUSIONS Hyperkinetic movement disorder is a key clinical feature in patients with CDG.
31132195	12	4	theme	developmental	1628:1640	arg1	delay					1642:1646	developmental delay	1628:1646	developmental delay	1628:1646	CDG should be considered in the differential diagnosis of childhood-onset dyskinesia, especially when associated with ataxia, developmental delay, intellectual disability, autism or seizure disorder.
31132195	11	5	dep	CONCLUSIONS	1411:1421	arg1	feature					1472:1478	a key clinical feature	1457:1478	a key clinical feature in patients with CDG	1457:1499	CONCLUSIONS Hyperkinetic movement disorder is a key clinical feature in patients with CDG.
31132195	11	5	dep	CONCLUSIONS	1411:1421	arg1	disorder					1445:1452	Hyperkinetic movement disorder	1423:1452	Hyperkinetic movement disorder	1423:1452	CONCLUSIONS Hyperkinetic movement disorder is a key clinical feature in patients with CDG.
31132195	3	6	theme	CDG	533:535	arg1	subtypes					537:544	CDG subtypes	533:544	CDG subtypes	533:544	The aim of the current study was to investigate hyperkinetic movement disorders in patients affected by CDG and to characterize phenomenology based on CDG subtypes.
31132195	10	7	theme	generalized	1319:1329	arg1	chorea					1331:1336	pure generalized chorea	1314:1336	pure generalized chorea	1314:1336	In patients affected by other CDG types, the movement disorders ranged from pure generalized chorea to mixed movement disorders including dystonia and complex stereotypies.
31132195	4	8	theme	patients	597:604	arg1	cohort					587:592	a cohort	585:592	a cohort of patients with CDG who were referred to the University Hospital of Catania, Italy	585:676	METHODS Subjects were identified from a cohort of patients with CDG who were referred to the University Hospital of Catania, Italy.
31132195	12	9	theme	intellectual	1649:1660	arg1	disability					1662:1671	intellectual disability	1649:1671	intellectual disability	1649:1671	CDG should be considered in the differential diagnosis of childhood-onset dyskinesia, especially when associated with ataxia, developmental delay, intellectual disability, autism or seizure disorder.
31132195	3	10	theme	study	405:409	arg1	aim					386:388	The aim	382:388	The aim of the current study	382:409	The aim of the current study was to investigate hyperkinetic movement disorders in patients affected by CDG and to characterize phenomenology based on CDG subtypes.
31132195	4	11	theme	Italy	672:676	arg1	Hospital					651:658	the University Hospital	636:658	the University Hospital of Catania, Italy	636:676	METHODS Subjects were identified from a cohort of patients with CDG who were referred to the University Hospital of Catania, Italy.
31132195	6	12	theme	movement	836:843	arg1	disorders					845:853	hyperkinetic movement disorders	823:853	hyperkinetic movement disorders	823:853	RESULTS A variety of hyperkinetic movement disorders was detected in eight unrelated CDG patients.
31132195	1	13	theme	BACKGROUND	74:83	arg1	disorders					108:116	BACKGROUND AND PURPOSE Congenital disorders	74:116	BACKGROUND AND PURPOSE Congenital disorders of glycosylation (CDG)	74:139	BACKGROUND AND PURPOSE Congenital disorders of glycosylation (CDG) represent an increasing number of rare inherited metabolic diseases associated with abnormal glycan metabolism and disease onset in infancy or early childhood.
31132195	1	14	theme	early	284:288	arg1	childhood					290:298	early childhood	284:298	early childhood	284:298	BACKGROUND AND PURPOSE Congenital disorders of glycosylation (CDG) represent an increasing number of rare inherited metabolic diseases associated with abnormal glycan metabolism and disease onset in infancy or early childhood.
31132195	5	15	with	neurologists	706:717	arg1	expertise					724:732	expertise	724:732	expertise	724:732	Patients were evaluated by neurologists with expertise in movement disorders and videotaped using a standardized protocol.
31132195	3	16	theme	movement	443:450	arg1	disorders					452:460	hyperkinetic movement disorders	430:460	hyperkinetic movement disorders in patients affected by CDG	430:488	The aim of the current study was to investigate hyperkinetic movement disorders in patients affected by CDG and to characterize phenomenology based on CDG subtypes.
31132195	1	17	theme	rare	175:178	arg1	diseases					200:207	rare inherited metabolic diseases	175:207	rare inherited metabolic diseases associated with abnormal glycan metabolism and disease onset in infancy or early childhood	175:298	BACKGROUND AND PURPOSE Congenital disorders of glycosylation (CDG) represent an increasing number of rare inherited metabolic diseases associated with abnormal glycan metabolism and disease onset in infancy or early childhood.
31132195	10	18	theme	CDG	1268:1270	arg1	types					1272:1276	other CDG types	1262:1276	other CDG types	1262:1276	In patients affected by other CDG types, the movement disorders ranged from pure generalized chorea to mixed movement disorders including dystonia and complex stereotypies.
31132195	8	19	theme	segmental/multifocal	1088:1107	arg1	involvement					1109:1119	a segmental/multifocal involvement	1086:1119	a segmental/multifocal involvement	1086:1119	Distribution ranged from a generalized, especially in younger subjects, to a segmental/multifocal involvement.
31132195	7	20	theme	clinical	981:988	arg1	stability					990:998	a clinical stability	979:998	a clinical stability over time	979:1008	Involuntary movements were generally observed early in childhood, maintaining a clinical stability over time.
31132195	1	21	theme	PURPOSE	89:95	arg1	disorders					108:116	BACKGROUND AND PURPOSE Congenital disorders	74:116	BACKGROUND AND PURPOSE Congenital disorders of glycosylation (CDG)	74:139	BACKGROUND AND PURPOSE Congenital disorders of glycosylation (CDG) represent an increasing number of rare inherited metabolic diseases associated with abnormal glycan metabolism and disease onset in infancy or early childhood.
31132195	9	22	theme	phosphomannomutase	1139:1156	arg1	CDG					1160:1162	phosphomannomutase 2 CDG	1139:1162	phosphomannomutase 2 CDG	1139:1162	In patients with phosphomannomutase 2 CDG, the principal movement disorders included dystonia and choreo-athetosis.
31132195	1	23	theme	inherited	180:188	arg1	diseases					200:207	rare inherited metabolic diseases	175:207	rare inherited metabolic diseases associated with abnormal glycan metabolism and disease onset in infancy or early childhood	175:298	BACKGROUND AND PURPOSE Congenital disorders of glycosylation (CDG) represent an increasing number of rare inherited metabolic diseases associated with abnormal glycan metabolism and disease onset in infancy or early childhood.
31132195	0	24	theme	movement	13:20	arg1	disorders					22:30	Hyperkinetic movement disorders	0:30	Hyperkinetic movement disorders in congenital disorders of glycosylation	0:71	Hyperkinetic movement disorders in congenital disorders of glycosylation.
31132195	1	25	theme	Congenital	97:106	arg1	disorders					108:116	BACKGROUND AND PURPOSE Congenital disorders	74:116	BACKGROUND AND PURPOSE Congenital disorders of glycosylation (CDG)	74:139	BACKGROUND AND PURPOSE Congenital disorders of glycosylation (CDG) represent an increasing number of rare inherited metabolic diseases associated with abnormal glycan metabolism and disease onset in infancy or early childhood.
31132195	10	26	theme	mixed	1341:1345	arg1	disorders					1356:1364	mixed movement disorders	1341:1364	mixed movement disorders including dystonia and complex stereotypies	1341:1408	In patients affected by other CDG types, the movement disorders ranged from pure generalized chorea to mixed movement disorders including dystonia and complex stereotypies.
31132195	10	26	theme	mixed	1341:1345	arg1	dystonia					1376:1383	dystonia	1376:1383	dystonia	1376:1383	In patients affected by other CDG types, the movement disorders ranged from pure generalized chorea to mixed movement disorders including dystonia and complex stereotypies.
31132195	10	26	theme	mixed	1341:1345	arg1	stereotypies					1397:1408	complex stereotypies	1389:1408	complex stereotypies	1389:1408	In patients affected by other CDG types, the movement disorders ranged from pure generalized chorea to mixed movement disorders including dystonia and complex stereotypies.
31132195	1	27	theme	metabolic	190:198	arg1	diseases					200:207	rare inherited metabolic diseases	175:207	rare inherited metabolic diseases associated with abnormal glycan metabolism and disease onset in infancy or early childhood	175:298	BACKGROUND AND PURPOSE Congenital disorders of glycosylation (CDG) represent an increasing number of rare inherited metabolic diseases associated with abnormal glycan metabolism and disease onset in infancy or early childhood.
31132195	0	28	theme	Hyperkinetic	0:11	arg1	disorders					22:30	Hyperkinetic movement disorders	0:30	Hyperkinetic movement disorders in congenital disorders of glycosylation	0:71	Hyperkinetic movement disorders in congenital disorders of glycosylation.
31132195	8	29	theme	younger	1065:1071	arg1	subjects					1073:1080	younger subjects	1065:1080	younger subjects	1065:1080	Distribution ranged from a generalized, especially in younger subjects, to a segmental/multifocal involvement.
31132195	12	30	theme	dyskinesia	1576:1585	arg1	diagnosis					1547:1555	the differential diagnosis	1530:1555	the differential diagnosis of childhood-onset dyskinesia	1530:1585	CDG should be considered in the differential diagnosis of childhood-onset dyskinesia, especially when associated with ataxia, developmental delay, intellectual disability, autism or seizure disorder.
31132195	1	31	theme	diseases	200:207	arg1	number					165:170	an increasing number	151:170	an increasing number of rare inherited metabolic diseases associated with abnormal glycan metabolism and disease onset in infancy or early childhood	151:298	BACKGROUND AND PURPOSE Congenital disorders of glycosylation (CDG) represent an increasing number of rare inherited metabolic diseases associated with abnormal glycan metabolism and disease onset in infancy or early childhood.
31132195	6	32	theme	hyperkinetic	823:834	arg1	disorders					845:853	hyperkinetic movement disorders	823:853	hyperkinetic movement disorders	823:853	RESULTS A variety of hyperkinetic movement disorders was detected in eight unrelated CDG patients.
31132195	2	33	theme	multisystemic	314:326	arg1	CDG					306:308	CDG	306:308	CDG	306:308	Most CDG are multisystemic diseases mainly affecting the central nervous system.
31132195	2	33	theme	multisystemic	314:326	arg1	diseases					328:335	multisystemic diseases	314:335	multisystemic diseases mainly affecting the central nervous system	314:379	Most CDG are multisystemic diseases mainly affecting the central nervous system.
31132195	10	34	theme	complex	1389:1395	arg1	stereotypies					1397:1408	complex stereotypies	1389:1408	complex stereotypies	1389:1408	In patients affected by other CDG types, the movement disorders ranged from pure generalized chorea to mixed movement disorders including dystonia and complex stereotypies.
31132195	1	35	theme	glycosylation	121:133	arg1	disorders					108:116	BACKGROUND AND PURPOSE Congenital disorders	74:116	BACKGROUND AND PURPOSE Congenital disorders of glycosylation (CDG)	74:139	BACKGROUND AND PURPOSE Congenital disorders of glycosylation (CDG) represent an increasing number of rare inherited metabolic diseases associated with abnormal glycan metabolism and disease onset in infancy or early childhood.
31132195	4	36	theme	University	640:649	arg1	Hospital					651:658	the University Hospital	636:658	the University Hospital of Catania, Italy	636:676	METHODS Subjects were identified from a cohort of patients with CDG who were referred to the University Hospital of Catania, Italy.
31132195	6	37	dep	RESULTS	802:808	arg1	detected					859:866	detected	859:866	was detected in eight unrelated CDG patients	855:898	RESULTS A variety of hyperkinetic movement disorders was detected in eight unrelated CDG patients.
31132195	9	38	theme	principal	1169:1177	arg1	disorders					1188:1196	the principal movement disorders	1165:1196	the principal movement disorders	1165:1196	In patients with phosphomannomutase 2 CDG, the principal movement disorders included dystonia and choreo-athetosis.
31132195	1	39	theme	abnormal	225:232	arg1	metabolism					241:250	abnormal glycan metabolism	225:250	abnormal glycan metabolism	225:250	BACKGROUND AND PURPOSE Congenital disorders of glycosylation (CDG) represent an increasing number of rare inherited metabolic diseases associated with abnormal glycan metabolism and disease onset in infancy or early childhood.
31132195	0	40	theme	congenital	35:44	arg1	disorders					46:54	congenital disorders	35:54	congenital disorders of glycosylation	35:71	Hyperkinetic movement disorders in congenital disorders of glycosylation.
31132195	12	41	theme	seizure	1684:1690	arg1	disorder					1692:1699	seizure disorder	1684:1699	seizure disorder	1684:1699	CDG should be considered in the differential diagnosis of childhood-onset dyskinesia, especially when associated with ataxia, developmental delay, intellectual disability, autism or seizure disorder.
31132195	3	42	theme	hyperkinetic	430:441	arg1	disorders					452:460	hyperkinetic movement disorders	430:460	hyperkinetic movement disorders in patients affected by CDG	430:488	The aim of the current study was to investigate hyperkinetic movement disorders in patients affected by CDG and to characterize phenomenology based on CDG subtypes.
31132195	9	43	theme	movement	1179:1186	arg1	disorders					1188:1196	the principal movement disorders	1165:1196	the principal movement disorders	1165:1196	In patients with phosphomannomutase 2 CDG, the principal movement disorders included dystonia and choreo-athetosis.
31132195	1	44	theme	glycan	234:239	arg1	metabolism					241:250	abnormal glycan metabolism	225:250	abnormal glycan metabolism	225:250	BACKGROUND AND PURPOSE Congenital disorders of glycosylation (CDG) represent an increasing number of rare inherited metabolic diseases associated with abnormal glycan metabolism and disease onset in infancy or early childhood.
31132195	0	45	theme	glycosylation	59:71	arg1	disorders					46:54	congenital disorders	35:54	congenital disorders of glycosylation	35:71	Hyperkinetic movement disorders in congenital disorders of glycosylation.
31132195	4	46	theme	METHODS	547:553	arg1	Subjects					555:562	METHODS Subjects	547:562	METHODS Subjects	547:562	METHODS Subjects were identified from a cohort of patients with CDG who were referred to the University Hospital of Catania, Italy.
31132195	6	47	theme	CDG	887:889	arg1	patients					891:898	eight unrelated CDG patients	871:898	eight unrelated CDG patients	871:898	RESULTS A variety of hyperkinetic movement disorders was detected in eight unrelated CDG patients.
31132195	10	48	theme	movement	1283:1290	arg1	disorders					1292:1300	the movement disorders	1279:1300	the movement disorders	1279:1300	In patients affected by other CDG types, the movement disorders ranged from pure generalized chorea to mixed movement disorders including dystonia and complex stereotypies.
31132195	2	49	theme	nervous	366:372	arg1	system					374:379	the central nervous system	354:379	the central nervous system	354:379	Most CDG are multisystemic diseases mainly affecting the central nervous system.
31132195	5	50	theme	movement	737:744	arg1	disorders					746:754	movement disorders	737:754	movement disorders	737:754	Patients were evaluated by neurologists with expertise in movement disorders and videotaped using a standardized protocol.
31132195	1	51	from	onset	264:268	arg1	infancy					273:279	infancy	273:279	infancy	273:279	BACKGROUND AND PURPOSE Congenital disorders of glycosylation (CDG) represent an increasing number of rare inherited metabolic diseases associated with abnormal glycan metabolism and disease onset in infancy or early childhood.
31132195	1	51	from	onset	264:268	arg1	childhood					290:298	early childhood	284:298	early childhood	284:298	BACKGROUND AND PURPOSE Congenital disorders of glycosylation (CDG) represent an increasing number of rare inherited metabolic diseases associated with abnormal glycan metabolism and disease onset in infancy or early childhood.
31132195	6	52	theme	unrelated	877:885	arg1	patients					891:898	eight unrelated CDG patients	871:898	eight unrelated CDG patients	871:898	RESULTS A variety of hyperkinetic movement disorders was detected in eight unrelated CDG patients.
31132195	12	53	theme	childhood-onset	1560:1574	arg1	dyskinesia					1576:1585	childhood-onset dyskinesia	1560:1585	childhood-onset dyskinesia	1560:1585	CDG should be considered in the differential diagnosis of childhood-onset dyskinesia, especially when associated with ataxia, developmental delay, intellectual disability, autism or seizure disorder.
31132195	2	54	theme	central	358:364	arg1	system					374:379	the central nervous system	354:379	the central nervous system	354:379	Most CDG are multisystemic diseases mainly affecting the central nervous system.
31132195	11	55	with	patients	1483:1490	arg1	CDG					1497:1499	CDG	1497:1499	CDG	1497:1499	CONCLUSIONS Hyperkinetic movement disorder is a key clinical feature in patients with CDG.
31132195	1	56	from	metabolism	241:250	arg1	infancy					273:279	infancy	273:279	infancy	273:279	BACKGROUND AND PURPOSE Congenital disorders of glycosylation (CDG) represent an increasing number of rare inherited metabolic diseases associated with abnormal glycan metabolism and disease onset in infancy or early childhood.
31132195	1	56	from	metabolism	241:250	arg1	childhood					290:298	early childhood	284:298	early childhood	284:298	BACKGROUND AND PURPOSE Congenital disorders of glycosylation (CDG) represent an increasing number of rare inherited metabolic diseases associated with abnormal glycan metabolism and disease onset in infancy or early childhood.
31132195	11	57	from	feature	1472:1478	arg1	patients					1483:1490	patients	1483:1490	patients with CDG	1483:1499	CONCLUSIONS Hyperkinetic movement disorder is a key clinical feature in patients with CDG.
31132195	6	58	theme	disorders	845:853	arg1	variety					812:818	A variety	810:818	A variety of hyperkinetic movement disorders	810:853	RESULTS A variety of hyperkinetic movement disorders was detected in eight unrelated CDG patients.
31132195	6	58	theme	disorders	845:853	arg1	disorders					845:853	hyperkinetic movement disorders	823:853	hyperkinetic movement disorders	823:853	RESULTS A variety of hyperkinetic movement disorders was detected in eight unrelated CDG patients.
31132195	10	59	theme	other	1262:1266	arg1	types					1272:1276	other CDG types	1262:1276	other CDG types	1262:1276	In patients affected by other CDG types, the movement disorders ranged from pure generalized chorea to mixed movement disorders including dystonia and complex stereotypies.
31132195	11	60	theme	Hyperkinetic	1423:1434	arg1	feature					1472:1478	a key clinical feature	1457:1478	a key clinical feature in patients with CDG	1457:1499	CONCLUSIONS Hyperkinetic movement disorder is a key clinical feature in patients with CDG.
31132195	11	60	theme	Hyperkinetic	1423:1434	arg1	disorder					1445:1452	Hyperkinetic movement disorder	1423:1452	Hyperkinetic movement disorder	1423:1452	CONCLUSIONS Hyperkinetic movement disorder is a key clinical feature in patients with CDG.
31132195	9	61	with	patients	1125:1132	arg1	CDG					1160:1162	phosphomannomutase 2 CDG	1139:1162	phosphomannomutase 2 CDG	1139:1162	In patients with phosphomannomutase 2 CDG, the principal movement disorders included dystonia and choreo-athetosis.
31132195	7	62	theme	Involuntary	901:911	arg1	movements					913:921	Involuntary movements	901:921	Involuntary movements	901:921	Involuntary movements were generally observed early in childhood, maintaining a clinical stability over time.
31132195	11	63	theme	key	1459:1461	arg1	feature					1472:1478	a key clinical feature	1457:1478	a key clinical feature in patients with CDG	1457:1499	CONCLUSIONS Hyperkinetic movement disorder is a key clinical feature in patients with CDG.
31132195	11	63	theme	key	1459:1461	arg1	disorder					1445:1452	Hyperkinetic movement disorder	1423:1452	Hyperkinetic movement disorder	1423:1452	CONCLUSIONS Hyperkinetic movement disorder is a key clinical feature in patients with CDG.
31132195	10	64	theme	movement	1347:1354	arg1	disorders					1356:1364	mixed movement disorders	1341:1364	mixed movement disorders including dystonia and complex stereotypies	1341:1408	In patients affected by other CDG types, the movement disorders ranged from pure generalized chorea to mixed movement disorders including dystonia and complex stereotypies.
31132195	10	64	theme	movement	1347:1354	arg1	dystonia					1376:1383	dystonia	1376:1383	dystonia	1376:1383	In patients affected by other CDG types, the movement disorders ranged from pure generalized chorea to mixed movement disorders including dystonia and complex stereotypies.
31132195	10	64	theme	movement	1347:1354	arg1	stereotypies					1397:1408	complex stereotypies	1389:1408	complex stereotypies	1389:1408	In patients affected by other CDG types, the movement disorders ranged from pure generalized chorea to mixed movement disorders including dystonia and complex stereotypies.
31132195	10	65	theme	pure	1314:1317	arg1	chorea					1331:1336	pure generalized chorea	1314:1336	pure generalized chorea	1314:1336	In patients affected by other CDG types, the movement disorders ranged from pure generalized chorea to mixed movement disorders including dystonia and complex stereotypies.
31132195	3	66	from	disorders	452:460	arg1	patients					465:472	patients	465:472	patients affected by CDG	465:488	The aim of the current study was to investigate hyperkinetic movement disorders in patients affected by CDG and to characterize phenomenology based on CDG subtypes.
31132195	3	67	theme	current	397:403	arg1	study					405:409	the current study	393:409	the current study	393:409	The aim of the current study was to investigate hyperkinetic movement disorders in patients affected by CDG and to characterize phenomenology based on CDG subtypes.
31132195	1	68	theme	disease	256:262	arg1	onset					264:268	disease onset	256:268	disease onset	256:268	BACKGROUND AND PURPOSE Congenital disorders of glycosylation (CDG) represent an increasing number of rare inherited metabolic diseases associated with abnormal glycan metabolism and disease onset in infancy or early childhood.
31132195	5	69	theme	standardized	779:790	arg1	protocol					792:799	a standardized protocol	777:799	a standardized protocol	777:799	Patients were evaluated by neurologists with expertise in movement disorders and videotaped using a standardized protocol.
31132195	12	70	theme	differential	1534:1545	arg1	diagnosis					1547:1555	the differential diagnosis	1530:1555	the differential diagnosis of childhood-onset dyskinesia	1530:1585	CDG should be considered in the differential diagnosis of childhood-onset dyskinesia, especially when associated with ataxia, developmental delay, intellectual disability, autism or seizure disorder.
31132195	4	71	with	patients	597:604	arg1	CDG					611:613	CDG	611:613	CDG	611:613	METHODS Subjects were identified from a cohort of patients with CDG who were referred to the University Hospital of Catania, Italy.
31132195	1	72	theme	increasing	154:163	arg1	number					165:170	an increasing number	151:170	an increasing number of rare inherited metabolic diseases associated with abnormal glycan metabolism and disease onset in infancy or early childhood	151:298	BACKGROUND AND PURPOSE Congenital disorders of glycosylation (CDG) represent an increasing number of rare inherited metabolic diseases associated with abnormal glycan metabolism and disease onset in infancy or early childhood.
30905720	0	0	link	N-linked	84:91	arg1	proteins					112:119	the N-linked glycosylated venom proteins	80:119	the N-linked glycosylated venom proteins	80:119	Revisiting Polybia paulista wasp venom using shotgun proteomics - Insights into the N-linked glycosylated venom proteins.
30905720	4	1	theme	typical	901:907	arg1	proteins					915:922	(i) typical venom proteins	897:922	an extensive repertoire of hitherto undescribed proteins that were classified into the following six different functional groups: (i) typical venom proteins; (ii) proteins related to the folding/conformation and PTMs of toxins; (iii) proteins that protect toxins from oxidative stress	767:1050	This study reveals an extensive repertoire of hitherto undescribed proteins that were classified into the following six different functional groups: (i) typical venom proteins; (ii) proteins related to the folding/conformation and PTMs of toxins; (iii) proteins that protect toxins from oxidative stress; (iv) proteins involved in chemical communication; (v) housekeeping proteins; and (vi) uncharacterized proteins.
30905720	8	2	theme	novel	1809:1813	arg1	neurotoxins					1826:1836	novel wasp venom neurotoxins	1809:1836	novel wasp venom neurotoxins	1809:1836	For example, novel wasp venom neurotoxins have been identified, but no studies have assessed the presence of this type of toxin in social wasp venoms.
30905720	9	3	theme	glycosylated	1972:1983	arg1	proteins					1991:1998	23 N-linked glycosylated venom proteins	1960:1998	23 N-linked glycosylated venom proteins	1960:1998	In addition, 23 N-linked glycosylated venom proteins were identified in the P. paulista venom proteome, and some of these proteins might be relevant allergens that are immunoreactive to human IgE.
30905720	4	4	theme	oxidative	1035:1043	arg1	stress					1045:1050	oxidative stress	1035:1050	oxidative stress	1035:1050	This study reveals an extensive repertoire of hitherto undescribed proteins that were classified into the following six different functional groups: (i) typical venom proteins; (ii) proteins related to the folding/conformation and PTMs of toxins; (iii) proteins that protect toxins from oxidative stress; (iv) proteins involved in chemical communication; (v) housekeeping proteins; and (vi) uncharacterized proteins.
30905720	3	5	theme	N-linked	724:731	arg1	glycosylation					733:745	N-linked glycosylation	724:745	N-linked glycosylation	724:745	Three different molecular forms of PLA1 were identified as allergenic proteins, and two of these forms were modified by N-linked glycosylation.
30905720	8	6	theme	venom	1820:1824	arg1	neurotoxins					1826:1836	novel wasp venom neurotoxins	1809:1836	novel wasp venom neurotoxins	1809:1836	For example, novel wasp venom neurotoxins have been identified, but no studies have assessed the presence of this type of toxin in social wasp venoms.
30905720	4	7	theme	functional	878:887	arg1	groups					889:894	the following six different functional groups	850:894	the following six different functional groups	850:894	This study reveals an extensive repertoire of hitherto undescribed proteins that were classified into the following six different functional groups: (i) typical venom proteins; (ii) proteins related to the folding/conformation and PTMs of toxins; (iii) proteins that protect toxins from oxidative stress; (iv) proteins involved in chemical communication; (v) housekeeping proteins; and (vi) uncharacterized proteins.
30905720	7	8	theme	mechanism	1737:1745	arg1	understanding					1705:1717	our understanding	1701:1717	our understanding of the envenoming mechanism produced by sting accidents caused by this wasp	1701:1793	BIOLOGICAL SIGNIFICANCE: The present study significantly expanded the number of proteins identified in P. paulista venom, contributing to improvements in our understanding of the envenoming mechanism produced by sting accidents caused by this wasp.
30905720	7	9	from	improvements	1685:1696	arg1	understanding					1705:1717	our understanding	1701:1717	our understanding of the envenoming mechanism produced by sting accidents caused by this wasp	1701:1793	BIOLOGICAL SIGNIFICANCE: The present study significantly expanded the number of proteins identified in P. paulista venom, contributing to improvements in our understanding of the envenoming mechanism produced by sting accidents caused by this wasp.
30905720	9	10	theme	relevant	2087:2094	arg1	allergens					2096:2104	relevant allergens	2087:2104	relevant allergens that are immunoreactive to human IgE	2087:2141	In addition, 23 N-linked glycosylated venom proteins were identified in the P. paulista venom proteome, and some of these proteins might be relevant allergens that are immunoreactive to human IgE.
30905720	2	11	theme	N-linked	544:551	arg1	modification					590:601	a posttranslational modification	570:601	a posttranslational modification	570:601	Here, we reinvestigated the P. paulista venom using a gel-free shotgun proteomic approach; the highly dynamic range of this approach facilitated the detection and identification of 1673 proteins, of which 23 venom proteins presented N-linked glycosylation as a posttranslational modification.
30905720	2	11	theme	N-linked	544:551	arg1	glycosylation					553:565	N-linked glycosylation	544:565	N-linked glycosylation	544:565	Here, we reinvestigated the P. paulista venom using a gel-free shotgun proteomic approach; the highly dynamic range of this approach facilitated the detection and identification of 1673 proteins, of which 23 venom proteins presented N-linked glycosylation as a posttranslational modification.
30905720	4	12	dep	involved	1067:1074	arg1	iv					1054:1055	iv	1054:1055	iv	1054:1055	This study reveals an extensive repertoire of hitherto undescribed proteins that were classified into the following six different functional groups: (i) typical venom proteins; (ii) proteins related to the folding/conformation and PTMs of toxins; (iii) proteins that protect toxins from oxidative stress; (iv) proteins involved in chemical communication; (v) housekeeping proteins; and (vi) uncharacterized proteins.
30905720	6	13	theme	envenoming	1446:1455	arg1	mechanism					1457:1465	its envenoming mechanism	1442:1465	its envenoming mechanism	1442:1465	Thus, the findings reported here may contribute to improving our understanding of the composition of P. paulista venom, its envenoming mechanism and the pathologies experienced by the victim after the wasp stinging accident.
30905720	3	14	theme	different	610:618	arg1	forms					630:634	Three different molecular forms	604:634	Three different molecular forms of PLA1	604:642	Three different molecular forms of PLA1 were identified as allergenic proteins, and two of these forms were modified by N-linked glycosylation.
30905720	3	14	theme	different	610:618	arg1	proteins					674:681	allergenic proteins	663:681	allergenic proteins	663:681	Three different molecular forms of PLA1 were identified as allergenic proteins, and two of these forms were modified by N-linked glycosylation.
30905720	1	15	theme	gel-dependent	216:228	arg1	approach					230:237	a gel-dependent approach	214:237	a gel-dependent approach	214:237	The partial proteome of Polybia paulista wasp venom was previously reported elsewhere using a gel-dependent approach and resulted in the identification of a limited number of venom toxins.
30905720	2	16	theme	posttranslational	572:588	arg1	modification					590:601	a posttranslational modification	570:601	a posttranslational modification	570:601	Here, we reinvestigated the P. paulista venom using a gel-free shotgun proteomic approach; the highly dynamic range of this approach facilitated the detection and identification of 1673 proteins, of which 23 venom proteins presented N-linked glycosylation as a posttranslational modification.
30905720	2	16	theme	posttranslational	572:588	arg1	glycosylation					553:565	N-linked glycosylation	544:565	N-linked glycosylation	544:565	Here, we reinvestigated the P. paulista venom using a gel-free shotgun proteomic approach; the highly dynamic range of this approach facilitated the detection and identification of 1673 proteins, of which 23 venom proteins presented N-linked glycosylation as a posttranslational modification.
30905720	9	17	theme	human	2133:2137	arg1	IgE					2139:2141	human IgE	2133:2141	human IgE	2133:2141	In addition, 23 N-linked glycosylated venom proteins were identified in the P. paulista venom proteome, and some of these proteins might be relevant allergens that are immunoreactive to human IgE.
30905720	6	18	theme	stinging	1528:1535	arg1	accident					1537:1544	the wasp stinging accident	1519:1544	the wasp stinging accident	1519:1544	Thus, the findings reported here may contribute to improving our understanding of the composition of P. paulista venom, its envenoming mechanism and the pathologies experienced by the victim after the wasp stinging accident.
30905720	4	19	theme	proteins	915:922	arg1	proteins					815:822	hitherto undescribed proteins	794:822	hitherto undescribed proteins	794:822	This study reveals an extensive repertoire of hitherto undescribed proteins that were classified into the following six different functional groups: (i) typical venom proteins; (ii) proteins related to the folding/conformation and PTMs of toxins; (iii) proteins that protect toxins from oxidative stress; (iv) proteins involved in chemical communication; (v) housekeeping proteins; and (vi) uncharacterized proteins.
30905720	4	19	theme	proteins	915:922	arg1	repertoire					780:789	an extensive repertoire	767:789	an extensive repertoire of hitherto undescribed proteins that were classified into the following six different functional groups: (i) typical venom proteins; (ii) proteins related to the folding/conformation and PTMs of toxins; (iii) proteins that protect toxins from oxidative stress	767:1050	This study reveals an extensive repertoire of hitherto undescribed proteins that were classified into the following six different functional groups: (i) typical venom proteins; (ii) proteins related to the folding/conformation and PTMs of toxins; (iii) proteins that protect toxins from oxidative stress; (iv) proteins involved in chemical communication; (v) housekeeping proteins; and (vi) uncharacterized proteins.
30905720	4	20	dep	folding/conformation	954:973	arg1	the					950:952	the	950:952	the	950:952	This study reveals an extensive repertoire of hitherto undescribed proteins that were classified into the following six different functional groups: (i) typical venom proteins; (ii) proteins related to the folding/conformation and PTMs of toxins; (iii) proteins that protect toxins from oxidative stress; (iv) proteins involved in chemical communication; (v) housekeeping proteins; and (vi) uncharacterized proteins.
30905720	3	21	theme	PLA1	639:642	arg1	forms					630:634	Three different molecular forms	604:634	Three different molecular forms of PLA1	604:642	Three different molecular forms of PLA1 were identified as allergenic proteins, and two of these forms were modified by N-linked glycosylation.
30905720	3	21	theme	PLA1	639:642	arg1	proteins					674:681	allergenic proteins	663:681	allergenic proteins	663:681	Three different molecular forms of PLA1 were identified as allergenic proteins, and two of these forms were modified by N-linked glycosylation.
30905720	2	22	theme	proteins	497:504	arg1	identification					474:487	identification	474:487	identification	474:487	Here, we reinvestigated the P. paulista venom using a gel-free shotgun proteomic approach; the highly dynamic range of this approach facilitated the detection and identification of 1673 proteins, of which 23 venom proteins presented N-linked glycosylation as a posttranslational modification.
30905720	2	22	theme	proteins	497:504	arg1	detection					460:468	detection	460:468	detection	460:468	Here, we reinvestigated the P. paulista venom using a gel-free shotgun proteomic approach; the highly dynamic range of this approach facilitated the detection and identification of 1673 proteins, of which 23 venom proteins presented N-linked glycosylation as a posttranslational modification.
30905720	4	23	dep	repertoire	780:789	arg1	related					939:945	related	939:945	related	939:945	This study reveals an extensive repertoire of hitherto undescribed proteins that were classified into the following six different functional groups: (i) typical venom proteins; (ii) proteins related to the folding/conformation and PTMs of toxins; (iii) proteins that protect toxins from oxidative stress; (iv) proteins involved in chemical communication; (v) housekeeping proteins; and (vi) uncharacterized proteins.
30905720	8	24	attach	presence	1893:1900	arg2	type					1910:1913	this type	1905:1913	this type of toxin	1905:1922	For example, novel wasp venom neurotoxins have been identified, but no studies have assessed the presence of this type of toxin in social wasp venoms.
30905720	8	24	attach	presence	1893:1900	arg1	venoms					1939:1944	social wasp venoms	1927:1944	social wasp venoms	1927:1944	For example, novel wasp venom neurotoxins have been identified, but no studies have assessed the presence of this type of toxin in social wasp venoms.
30905720	5	25	theme	toxin-like	1199:1208	arg1	proteins					1210:1217	venom toxin-like proteins	1193:1217	venom toxin-like proteins that are commonly reported in other animal venoms, including arthropods such as spiders and scorpions	1193:1319	It was possible to identify venom toxin-like proteins that are commonly reported in other animal venoms, including arthropods such as spiders and scorpions.
30905720	1	26	theme	wasp	163:166	arg1	venom					168:172	Polybia paulista wasp venom	146:172	Polybia paulista wasp venom	146:172	The partial proteome of Polybia paulista wasp venom was previously reported elsewhere using a gel-dependent approach and resulted in the identification of a limited number of venom toxins.
30905720	4	27	theme	extensive	770:778	arg1	proteins					815:822	hitherto undescribed proteins	794:822	hitherto undescribed proteins	794:822	This study reveals an extensive repertoire of hitherto undescribed proteins that were classified into the following six different functional groups: (i) typical venom proteins; (ii) proteins related to the folding/conformation and PTMs of toxins; (iii) proteins that protect toxins from oxidative stress; (iv) proteins involved in chemical communication; (v) housekeeping proteins; and (vi) uncharacterized proteins.
30905720	4	27	theme	extensive	770:778	arg1	repertoire					780:789	an extensive repertoire	767:789	an extensive repertoire of hitherto undescribed proteins that were classified into the following six different functional groups: (i) typical venom proteins; (ii) proteins related to the folding/conformation and PTMs of toxins; (iii) proteins that protect toxins from oxidative stress	767:1050	This study reveals an extensive repertoire of hitherto undescribed proteins that were classified into the following six different functional groups: (i) typical venom proteins; (ii) proteins related to the folding/conformation and PTMs of toxins; (iii) proteins that protect toxins from oxidative stress; (iv) proteins involved in chemical communication; (v) housekeeping proteins; and (vi) uncharacterized proteins.
30905720	0	28	theme	N-linked	84:91	arg1	proteins					112:119	the N-linked glycosylated venom proteins	80:119	the N-linked glycosylated venom proteins	80:119	Revisiting Polybia paulista wasp venom using shotgun proteomics - Insights into the N-linked glycosylated venom proteins.
30905720	2	29	dep	detection	460:468	arg1	the					456:458	the	456:458	the	456:458	Here, we reinvestigated the P. paulista venom using a gel-free shotgun proteomic approach; the highly dynamic range of this approach facilitated the detection and identification of 1673 proteins, of which 23 venom proteins presented N-linked glycosylation as a posttranslational modification.
30905720	7	30	theme	P.	1650:1651	arg1	venom					1662:1666	P. paulista venom	1650:1666	P. paulista venom	1650:1666	BIOLOGICAL SIGNIFICANCE: The present study significantly expanded the number of proteins identified in P. paulista venom, contributing to improvements in our understanding of the envenoming mechanism produced by sting accidents caused by this wasp.
30905720	8	31	theme	type	1910:1913	arg1	presence					1893:1900	the presence	1889:1900	the presence of this type of toxin in social wasp venoms	1889:1944	For example, novel wasp venom neurotoxins have been identified, but no studies have assessed the presence of this type of toxin in social wasp venoms.
30905720	0	32	theme	venom	106:110	arg1	proteins					112:119	the N-linked glycosylated venom proteins	80:119	the N-linked glycosylated venom proteins	80:119	Revisiting Polybia paulista wasp venom using shotgun proteomics - Insights into the N-linked glycosylated venom proteins.
30905720	2	33	theme	approach	435:442	arg1	range					421:425	the highly dynamic range	402:425	the highly dynamic range of this approach	402:442	Here, we reinvestigated the P. paulista venom using a gel-free shotgun proteomic approach; the highly dynamic range of this approach facilitated the detection and identification of 1673 proteins, of which 23 venom proteins presented N-linked glycosylation as a posttranslational modification.
30905720	4	34	theme	uncharacterized	1139:1153	arg1	proteins					1155:1162	(vi) uncharacterized proteins	1134:1162	(vi) uncharacterized proteins	1134:1162	This study reveals an extensive repertoire of hitherto undescribed proteins that were classified into the following six different functional groups: (i) typical venom proteins; (ii) proteins related to the folding/conformation and PTMs of toxins; (iii) proteins that protect toxins from oxidative stress; (iv) proteins involved in chemical communication; (v) housekeeping proteins; and (vi) uncharacterized proteins.
30905720	2	35	theme	shotgun	374:380	arg1	approach					392:399	a gel-free shotgun proteomic approach	363:399	a gel-free shotgun proteomic approach	363:399	Here, we reinvestigated the P. paulista venom using a gel-free shotgun proteomic approach; the highly dynamic range of this approach facilitated the detection and identification of 1673 proteins, of which 23 venom proteins presented N-linked glycosylation as a posttranslational modification.
30905720	1	36	theme	limited	279:285	arg1	number					287:292	a limited number	277:292	a limited number of venom toxins	277:308	The partial proteome of Polybia paulista wasp venom was previously reported elsewhere using a gel-dependent approach and resulted in the identification of a limited number of venom toxins.
30905720	0	37	gly	glycosylated	93:104	arg1	proteins					112:119	the N-linked glycosylated venom proteins	80:119	the N-linked glycosylated venom proteins	80:119	Revisiting Polybia paulista wasp venom using shotgun proteomics - Insights into the N-linked glycosylated venom proteins.
30905720	2	38	dep	P.	339:340	arg1	paulista					342:349	paulista	342:349	paulista	342:349	Here, we reinvestigated the P. paulista venom using a gel-free shotgun proteomic approach; the highly dynamic range of this approach facilitated the detection and identification of 1673 proteins, of which 23 venom proteins presented N-linked glycosylation as a posttranslational modification.
30905720	5	39	theme	animal	1255:1260	arg1	venoms					1262:1267	other animal venoms	1249:1267	other animal venoms	1249:1267	It was possible to identify venom toxin-like proteins that are commonly reported in other animal venoms, including arthropods such as spiders and scorpions.
30905720	5	39	theme	animal	1255:1260	arg1	arthropods					1280:1289	arthropods	1280:1289	arthropods such as spiders and scorpions	1280:1319	It was possible to identify venom toxin-like proteins that are commonly reported in other animal venoms, including arthropods such as spiders and scorpions.
30905720	2	40	theme	dynamic	413:419	arg1	range					421:425	the highly dynamic range	402:425	the highly dynamic range of this approach	402:442	Here, we reinvestigated the P. paulista venom using a gel-free shotgun proteomic approach; the highly dynamic range of this approach facilitated the detection and identification of 1673 proteins, of which 23 venom proteins presented N-linked glycosylation as a posttranslational modification.
30905720	0	41	theme	paulista	19:26	arg1	venom					33:37	Polybia paulista wasp venom	11:37	Polybia paulista wasp venom using shotgun proteomics	11:62	Revisiting Polybia paulista wasp venom using shotgun proteomics - Insights into the N-linked glycosylated venom proteins.
30905720	3	42	theme	allergenic	663:672	arg1	forms					630:634	Three different molecular forms	604:634	Three different molecular forms of PLA1	604:642	Three different molecular forms of PLA1 were identified as allergenic proteins, and two of these forms were modified by N-linked glycosylation.
30905720	3	42	theme	allergenic	663:672	arg1	proteins					674:681	allergenic proteins	663:681	allergenic proteins	663:681	Three different molecular forms of PLA1 were identified as allergenic proteins, and two of these forms were modified by N-linked glycosylation.
30905720	1	43	theme	toxins	303:308	arg1	number					287:292	a limited number	277:292	a limited number of venom toxins	277:308	The partial proteome of Polybia paulista wasp venom was previously reported elsewhere using a gel-dependent approach and resulted in the identification of a limited number of venom toxins.
30905720	2	44	theme	P.	339:340	arg1	venom					351:355	the P. paulista venom	335:355	the P. paulista venom	335:355	Here, we reinvestigated the P. paulista venom using a gel-free shotgun proteomic approach; the highly dynamic range of this approach facilitated the detection and identification of 1673 proteins, of which 23 venom proteins presented N-linked glycosylation as a posttranslational modification.
30905720	0	45	theme	shotgun	45:51	arg1	proteomics					53:62	shotgun proteomics	45:62	shotgun proteomics	45:62	Revisiting Polybia paulista wasp venom using shotgun proteomics - Insights into the N-linked glycosylated venom proteins.
30905720	4	46	theme	undescribed	803:813	arg1	proteins					815:822	hitherto undescribed proteins	794:822	hitherto undescribed proteins	794:822	This study reveals an extensive repertoire of hitherto undescribed proteins that were classified into the following six different functional groups: (i) typical venom proteins; (ii) proteins related to the folding/conformation and PTMs of toxins; (iii) proteins that protect toxins from oxidative stress; (iv) proteins involved in chemical communication; (v) housekeeping proteins; and (vi) uncharacterized proteins.
30905720	6	47	theme	venom	1435:1439	arg1	composition					1408:1418	the composition	1404:1418	the composition of P. paulista venom	1404:1439	Thus, the findings reported here may contribute to improving our understanding of the composition of P. paulista venom, its envenoming mechanism and the pathologies experienced by the victim after the wasp stinging accident.
30905720	1	48	theme	Polybia	146:152	arg1	venom					168:172	Polybia paulista wasp venom	146:172	Polybia paulista wasp venom	146:172	The partial proteome of Polybia paulista wasp venom was previously reported elsewhere using a gel-dependent approach and resulted in the identification of a limited number of venom toxins.
30905720	3	49	link	N-linked	724:731	arg1	glycosylation					733:745	N-linked glycosylation	724:745	N-linked glycosylation	724:745	Three different molecular forms of PLA1 were identified as allergenic proteins, and two of these forms were modified by N-linked glycosylation.
30905720	6	50	theme	P.	1423:1424	arg1	venom					1435:1439	P. paulista venom	1423:1439	P. paulista venom	1423:1439	Thus, the findings reported here may contribute to improving our understanding of the composition of P. paulista venom, its envenoming mechanism and the pathologies experienced by the victim after the wasp stinging accident.
30905720	8	51	theme	wasp	1815:1818	arg1	neurotoxins					1826:1836	novel wasp venom neurotoxins	1809:1836	novel wasp venom neurotoxins	1809:1836	For example, novel wasp venom neurotoxins have been identified, but no studies have assessed the presence of this type of toxin in social wasp venoms.
30905720	6	52	theme	composition	1408:1418	arg1	mechanism					1457:1465	its envenoming mechanism	1442:1465	its envenoming mechanism	1442:1465	Thus, the findings reported here may contribute to improving our understanding of the composition of P. paulista venom, its envenoming mechanism and the pathologies experienced by the victim after the wasp stinging accident.
30905720	6	52	theme	composition	1408:1418	arg1	pathologies					1475:1485	the pathologies	1471:1485	the pathologies experienced by the victim after the wasp stinging accident	1471:1544	Thus, the findings reported here may contribute to improving our understanding of the composition of P. paulista venom, its envenoming mechanism and the pathologies experienced by the victim after the wasp stinging accident.
30905720	6	52	theme	composition	1408:1418	arg1	understanding					1387:1399	our understanding	1383:1399	our understanding of the composition of P. paulista venom	1383:1439	Thus, the findings reported here may contribute to improving our understanding of the composition of P. paulista venom, its envenoming mechanism and the pathologies experienced by the victim after the wasp stinging accident.
30905720	8	53	from	presence	1893:1900	arg1	venoms					1939:1944	social wasp venoms	1927:1944	social wasp venoms	1927:1944	For example, novel wasp venom neurotoxins have been identified, but no studies have assessed the presence of this type of toxin in social wasp venoms.
30905720	4	54	dep	proteins	1120:1127	arg1	v					1104:1104	v	1104:1104	v	1104:1104	This study reveals an extensive repertoire of hitherto undescribed proteins that were classified into the following six different functional groups: (i) typical venom proteins; (ii) proteins related to the folding/conformation and PTMs of toxins; (iii) proteins that protect toxins from oxidative stress; (iv) proteins involved in chemical communication; (v) housekeeping proteins; and (vi) uncharacterized proteins.
30905720	1	55	theme	venom	168:172	arg1	proteome					134:141	The partial proteome	122:141	The partial proteome of Polybia paulista wasp venom	122:172	The partial proteome of Polybia paulista wasp venom was previously reported elsewhere using a gel-dependent approach and resulted in the identification of a limited number of venom toxins.
30905720	4	56	theme	following	854:862	arg1	groups					889:894	the following six different functional groups	850:894	the following six different functional groups	850:894	This study reveals an extensive repertoire of hitherto undescribed proteins that were classified into the following six different functional groups: (i) typical venom proteins; (ii) proteins related to the folding/conformation and PTMs of toxins; (iii) proteins that protect toxins from oxidative stress; (iv) proteins involved in chemical communication; (v) housekeeping proteins; and (vi) uncharacterized proteins.
30905720	7	57	theme	envenoming	1726:1735	arg1	mechanism					1737:1745	the envenoming mechanism	1722:1745	the envenoming mechanism produced by sting accidents caused by this wasp	1722:1793	BIOLOGICAL SIGNIFICANCE: The present study significantly expanded the number of proteins identified in P. paulista venom, contributing to improvements in our understanding of the envenoming mechanism produced by sting accidents caused by this wasp.
30905720	2	58	link	N-linked	544:551	arg1	modification					590:601	a posttranslational modification	570:601	a posttranslational modification	570:601	Here, we reinvestigated the P. paulista venom using a gel-free shotgun proteomic approach; the highly dynamic range of this approach facilitated the detection and identification of 1673 proteins, of which 23 venom proteins presented N-linked glycosylation as a posttranslational modification.
30905720	2	58	link	N-linked	544:551	arg1	glycosylation					553:565	N-linked glycosylation	544:565	N-linked glycosylation	544:565	Here, we reinvestigated the P. paulista venom using a gel-free shotgun proteomic approach; the highly dynamic range of this approach facilitated the detection and identification of 1673 proteins, of which 23 venom proteins presented N-linked glycosylation as a posttranslational modification.
30905720	4	59	theme	different	868:876	arg1	groups					889:894	the following six different functional groups	850:894	the following six different functional groups	850:894	This study reveals an extensive repertoire of hitherto undescribed proteins that were classified into the following six different functional groups: (i) typical venom proteins; (ii) proteins related to the folding/conformation and PTMs of toxins; (iii) proteins that protect toxins from oxidative stress; (iv) proteins involved in chemical communication; (v) housekeeping proteins; and (vi) uncharacterized proteins.
30905720	7	60	theme	present	1576:1582	arg1	study					1584:1588	The present study	1572:1588	The present study	1572:1588	BIOLOGICAL SIGNIFICANCE: The present study significantly expanded the number of proteins identified in P. paulista venom, contributing to improvements in our understanding of the envenoming mechanism produced by sting accidents caused by this wasp.
30905720	4	61	theme	chemical	1079:1086	arg1	communication					1088:1100	chemical communication	1079:1100	chemical communication	1079:1100	This study reveals an extensive repertoire of hitherto undescribed proteins that were classified into the following six different functional groups: (i) typical venom proteins; (ii) proteins related to the folding/conformation and PTMs of toxins; (iii) proteins that protect toxins from oxidative stress; (iv) proteins involved in chemical communication; (v) housekeeping proteins; and (vi) uncharacterized proteins.
30905720	4	62	dep	related	939:945	arg1	ii					926:927	ii	926:927	ii	926:927	This study reveals an extensive repertoire of hitherto undescribed proteins that were classified into the following six different functional groups: (i) typical venom proteins; (ii) proteins related to the folding/conformation and PTMs of toxins; (iii) proteins that protect toxins from oxidative stress; (iv) proteins involved in chemical communication; (v) housekeeping proteins; and (vi) uncharacterized proteins.
30905720	3	63	mod	modified	712:719	arg1	forms					701:705	these forms	695:705	these forms	695:705	Three different molecular forms of PLA1 were identified as allergenic proteins, and two of these forms were modified by N-linked glycosylation.
30905720	3	63	mod	modified	712:719	arg3	glycosylation					733:745	N-linked glycosylation	724:745	N-linked glycosylation	724:745	Three different molecular forms of PLA1 were identified as allergenic proteins, and two of these forms were modified by N-linked glycosylation.
30905720	3	63	mod	modified	712:719	arg1	two					688:690	two	688:690	two	688:690	Three different molecular forms of PLA1 were identified as allergenic proteins, and two of these forms were modified by N-linked glycosylation.
30905720	2	64	theme	venom	519:523	arg1	proteins					525:532	23 venom proteins	516:532	23 venom proteins	516:532	Here, we reinvestigated the P. paulista venom using a gel-free shotgun proteomic approach; the highly dynamic range of this approach facilitated the detection and identification of 1673 proteins, of which 23 venom proteins presented N-linked glycosylation as a posttranslational modification.
30905720	8	65	theme	social	1927:1932	arg1	venoms					1939:1944	social wasp venoms	1927:1944	social wasp venoms	1927:1944	For example, novel wasp venom neurotoxins have been identified, but no studies have assessed the presence of this type of toxin in social wasp venoms.
30905720	7	66	theme	BIOLOGICAL	1547:1556	arg1	SIGNIFICANCE					1558:1569	BIOLOGICAL SIGNIFICANCE	1547:1569	BIOLOGICAL SIGNIFICANCE: The present study significantly expanded the number of proteins identified in P. paulista venom, contributing to improvements in our understanding of the envenoming mechanism produced by sting accidents caused by this wasp.	1547:1794	BIOLOGICAL SIGNIFICANCE: The present study significantly expanded the number of proteins identified in P. paulista venom, contributing to improvements in our understanding of the envenoming mechanism produced by sting accidents caused by this wasp.
30905720	4	67	dep	proteins	1155:1162	arg1	vi					1135:1136	vi	1135:1136	vi	1135:1136	This study reveals an extensive repertoire of hitherto undescribed proteins that were classified into the following six different functional groups: (i) typical venom proteins; (ii) proteins related to the folding/conformation and PTMs of toxins; (iii) proteins that protect toxins from oxidative stress; (iv) proteins involved in chemical communication; (v) housekeeping proteins; and (vi) uncharacterized proteins.
30905720	6	68	theme	wasp	1523:1526	arg1	accident					1537:1544	the wasp stinging accident	1519:1544	the wasp stinging accident	1519:1544	Thus, the findings reported here may contribute to improving our understanding of the composition of P. paulista venom, its envenoming mechanism and the pathologies experienced by the victim after the wasp stinging accident.
30905720	1	69	theme	partial	126:132	arg1	proteome					134:141	The partial proteome	122:141	The partial proteome of Polybia paulista wasp venom	122:172	The partial proteome of Polybia paulista wasp venom was previously reported elsewhere using a gel-dependent approach and resulted in the identification of a limited number of venom toxins.
30905720	4	70	theme	venom	909:913	arg1	proteins					915:922	(i) typical venom proteins	897:922	an extensive repertoire of hitherto undescribed proteins that were classified into the following six different functional groups: (i) typical venom proteins; (ii) proteins related to the folding/conformation and PTMs of toxins; (iii) proteins that protect toxins from oxidative stress	767:1050	This study reveals an extensive repertoire of hitherto undescribed proteins that were classified into the following six different functional groups: (i) typical venom proteins; (ii) proteins related to the folding/conformation and PTMs of toxins; (iii) proteins that protect toxins from oxidative stress; (iv) proteins involved in chemical communication; (v) housekeeping proteins; and (vi) uncharacterized proteins.
30905720	4	71	theme	housekeeping	1107:1118	arg1	proteins					1120:1127	housekeeping proteins	1107:1127	(v) housekeeping proteins	1103:1127	This study reveals an extensive repertoire of hitherto undescribed proteins that were classified into the following six different functional groups: (i) typical venom proteins; (ii) proteins related to the folding/conformation and PTMs of toxins; (iii) proteins that protect toxins from oxidative stress; (iv) proteins involved in chemical communication; (v) housekeeping proteins; and (vi) uncharacterized proteins.
30905720	4	72	dep	proteins	915:922	arg1	i					898:898	i	898:898	i	898:898	This study reveals an extensive repertoire of hitherto undescribed proteins that were classified into the following six different functional groups: (i) typical venom proteins; (ii) proteins related to the folding/conformation and PTMs of toxins; (iii) proteins that protect toxins from oxidative stress; (iv) proteins involved in chemical communication; (v) housekeeping proteins; and (vi) uncharacterized proteins.
30905720	3	73	theme	molecular	620:628	arg1	forms					630:634	Three different molecular forms	604:634	Three different molecular forms of PLA1	604:642	Three different molecular forms of PLA1 were identified as allergenic proteins, and two of these forms were modified by N-linked glycosylation.
30905720	3	73	theme	molecular	620:628	arg1	proteins					674:681	allergenic proteins	663:681	allergenic proteins	663:681	Three different molecular forms of PLA1 were identified as allergenic proteins, and two of these forms were modified by N-linked glycosylation.
30905720	9	74	link	23 N-linked	1960:1970	arg1	proteins					1991:1998	23 N-linked glycosylated venom proteins	1960:1998	23 N-linked glycosylated venom proteins	1960:1998	In addition, 23 N-linked glycosylated venom proteins were identified in the P. paulista venom proteome, and some of these proteins might be relevant allergens that are immunoreactive to human IgE.
30905720	2	75	theme	1673	492:495	arg1	proteins					497:504	1673 proteins	492:504	1673 proteins	492:504	Here, we reinvestigated the P. paulista venom using a gel-free shotgun proteomic approach; the highly dynamic range of this approach facilitated the detection and identification of 1673 proteins, of which 23 venom proteins presented N-linked glycosylation as a posttranslational modification.
30905720	5	76	theme	venom	1193:1197	arg1	proteins					1210:1217	venom toxin-like proteins	1193:1217	venom toxin-like proteins that are commonly reported in other animal venoms, including arthropods such as spiders and scorpions	1193:1319	It was possible to identify venom toxin-like proteins that are commonly reported in other animal venoms, including arthropods such as spiders and scorpions.
30905720	8	77	theme	toxin	1918:1922	arg1	type					1910:1913	this type	1905:1913	this type of toxin	1905:1922	For example, novel wasp venom neurotoxins have been identified, but no studies have assessed the presence of this type of toxin in social wasp venoms.
30905720	1	78	theme	paulista	154:161	arg1	venom					168:172	Polybia paulista wasp venom	146:172	Polybia paulista wasp venom	146:172	The partial proteome of Polybia paulista wasp venom was previously reported elsewhere using a gel-dependent approach and resulted in the identification of a limited number of venom toxins.
30905720	0	79	theme	glycosylated	93:104	arg1	proteins					112:119	the N-linked glycosylated venom proteins	80:119	the N-linked glycosylated venom proteins	80:119	Revisiting Polybia paulista wasp venom using shotgun proteomics - Insights into the N-linked glycosylated venom proteins.
30905720	9	80	dep	P.	2023:2024	arg1	paulista					2026:2033	paulista	2026:2033	paulista	2026:2033	In addition, 23 N-linked glycosylated venom proteins were identified in the P. paulista venom proteome, and some of these proteins might be relevant allergens that are immunoreactive to human IgE.
30905720	7	81	dep	SIGNIFICANCE	1558:1569	arg1	expanded					1604:1611	expanded	1604:1611	expanded	1604:1611	BIOLOGICAL SIGNIFICANCE: The present study significantly expanded the number of proteins identified in P. paulista venom, contributing to improvements in our understanding of the envenoming mechanism produced by sting accidents caused by this wasp.
30905720	7	82	theme	paulista	1653:1660	arg1	venom					1662:1666	P. paulista venom	1650:1666	P. paulista venom	1650:1666	BIOLOGICAL SIGNIFICANCE: The present study significantly expanded the number of proteins identified in P. paulista venom, contributing to improvements in our understanding of the envenoming mechanism produced by sting accidents caused by this wasp.
30905720	9	83	theme	23 N-linked	1960:1970	arg1	proteins					1991:1998	23 N-linked glycosylated venom proteins	1960:1998	23 N-linked glycosylated venom proteins	1960:1998	In addition, 23 N-linked glycosylated venom proteins were identified in the P. paulista venom proteome, and some of these proteins might be relevant allergens that are immunoreactive to human IgE.
30905720	4	84	theme	repertoire	780:789	arg1	proteins					1001:1008	an extensive repertoire of hitherto undescribed proteins that were classified into the following six different functional groups: (i) typical venom proteins; (ii) proteins related to the folding/conformation and PTMs of toxins; (iii) proteins	767:1008	an extensive repertoire of hitherto undescribed proteins that were classified into the following six different functional groups: (i) typical venom proteins; (ii) proteins related to the folding/conformation and PTMs of toxins; (iii) proteins that protect toxins from oxidative stress	767:1050	This study reveals an extensive repertoire of hitherto undescribed proteins that were classified into the following six different functional groups: (i) typical venom proteins; (ii) proteins related to the folding/conformation and PTMs of toxins; (iii) proteins that protect toxins from oxidative stress; (iv) proteins involved in chemical communication; (v) housekeeping proteins; and (vi) uncharacterized proteins.
30905720	2	85	theme	proteomic	382:390	arg1	approach					392:399	a gel-free shotgun proteomic approach	363:399	a gel-free shotgun proteomic approach	363:399	Here, we reinvestigated the P. paulista venom using a gel-free shotgun proteomic approach; the highly dynamic range of this approach facilitated the detection and identification of 1673 proteins, of which 23 venom proteins presented N-linked glycosylation as a posttranslational modification.
30905720	0	86	theme	Polybia	11:17	arg1	venom					33:37	Polybia paulista wasp venom	11:37	Polybia paulista wasp venom using shotgun proteomics	11:62	Revisiting Polybia paulista wasp venom using shotgun proteomics - Insights into the N-linked glycosylated venom proteins.
30905720	9	87	theme	venom	1985:1989	arg1	proteins					1991:1998	23 N-linked glycosylated venom proteins	1960:1998	23 N-linked glycosylated venom proteins	1960:1998	In addition, 23 N-linked glycosylated venom proteins were identified in the P. paulista venom proteome, and some of these proteins might be relevant allergens that are immunoreactive to human IgE.
30905720	5	88	theme	other	1249:1253	arg1	venoms					1262:1267	other animal venoms	1249:1267	other animal venoms	1249:1267	It was possible to identify venom toxin-like proteins that are commonly reported in other animal venoms, including arthropods such as spiders and scorpions.
30905720	5	88	theme	other	1249:1253	arg1	arthropods					1280:1289	arthropods	1280:1289	arthropods such as spiders and scorpions	1280:1319	It was possible to identify venom toxin-like proteins that are commonly reported in other animal venoms, including arthropods such as spiders and scorpions.
30905720	2	89	theme	gel-free	365:372	arg1	approach					392:399	a gel-free shotgun proteomic approach	363:399	a gel-free shotgun proteomic approach	363:399	Here, we reinvestigated the P. paulista venom using a gel-free shotgun proteomic approach; the highly dynamic range of this approach facilitated the detection and identification of 1673 proteins, of which 23 venom proteins presented N-linked glycosylation as a posttranslational modification.
30905720	0	90	theme	wasp	28:31	arg1	venom					33:37	Polybia paulista wasp venom	11:37	Polybia paulista wasp venom using shotgun proteomics	11:62	Revisiting Polybia paulista wasp venom using shotgun proteomics - Insights into the N-linked glycosylated venom proteins.
30905720	1	91	theme	number	287:292	arg1	identification					259:272	the identification	255:272	the identification of a limited number of venom toxins	255:308	The partial proteome of Polybia paulista wasp venom was previously reported elsewhere using a gel-dependent approach and resulted in the identification of a limited number of venom toxins.
30905720	7	92	theme	proteins	1627:1634	arg1	number					1617:1622	the number	1613:1622	the number of proteins identified in P. paulista venom	1613:1666	BIOLOGICAL SIGNIFICANCE: The present study significantly expanded the number of proteins identified in P. paulista venom, contributing to improvements in our understanding of the envenoming mechanism produced by sting accidents caused by this wasp.
30905720	9	93	gly	glycosylated	1972:1983	arg1	proteins					1991:1998	23 N-linked glycosylated venom proteins	1960:1998	23 N-linked glycosylated venom proteins	1960:1998	In addition, 23 N-linked glycosylated venom proteins were identified in the P. paulista venom proteome, and some of these proteins might be relevant allergens that are immunoreactive to human IgE.
30905720	1	94	theme	venom	297:301	arg1	toxins					303:308	venom toxins	297:308	venom toxins	297:308	The partial proteome of Polybia paulista wasp venom was previously reported elsewhere using a gel-dependent approach and resulted in the identification of a limited number of venom toxins.
30905720	4	95	theme	toxins	987:992	arg1	PTMs					979:982	PTMs	979:982	PTMs	979:982	This study reveals an extensive repertoire of hitherto undescribed proteins that were classified into the following six different functional groups: (i) typical venom proteins; (ii) proteins related to the folding/conformation and PTMs of toxins; (iii) proteins that protect toxins from oxidative stress; (iv) proteins involved in chemical communication; (v) housekeeping proteins; and (vi) uncharacterized proteins.
30905720	4	95	theme	toxins	987:992	arg1	folding/conformation					954:973	folding/conformation	954:973	folding/conformation	954:973	This study reveals an extensive repertoire of hitherto undescribed proteins that were classified into the following six different functional groups: (i) typical venom proteins; (ii) proteins related to the folding/conformation and PTMs of toxins; (iii) proteins that protect toxins from oxidative stress; (iv) proteins involved in chemical communication; (v) housekeeping proteins; and (vi) uncharacterized proteins.
30905720	8	96	theme	wasp	1934:1937	arg1	venoms					1939:1944	social wasp venoms	1927:1944	social wasp venoms	1927:1944	For example, novel wasp venom neurotoxins have been identified, but no studies have assessed the presence of this type of toxin in social wasp venoms.
30905720	9	97	theme	P.	2023:2024	arg1	proteome					2041:2048	the P. paulista venom proteome	2019:2048	the P. paulista venom proteome	2019:2048	In addition, 23 N-linked glycosylated venom proteins were identified in the P. paulista venom proteome, and some of these proteins might be relevant allergens that are immunoreactive to human IgE.
30905720	9	98	theme	venom	2035:2039	arg1	proteome					2041:2048	the P. paulista venom proteome	2019:2048	the P. paulista venom proteome	2019:2048	In addition, 23 N-linked glycosylated venom proteins were identified in the P. paulista venom proteome, and some of these proteins might be relevant allergens that are immunoreactive to human IgE.
30905720	6	99	theme	paulista	1426:1433	arg1	venom					1435:1439	P. paulista venom	1423:1439	P. paulista venom	1423:1439	Thus, the findings reported here may contribute to improving our understanding of the composition of P. paulista venom, its envenoming mechanism and the pathologies experienced by the victim after the wasp stinging accident.
30905720	3	100	theme	forms	701:705	arg1	forms					701:705	these forms	695:705	these forms	695:705	Three different molecular forms of PLA1 were identified as allergenic proteins, and two of these forms were modified by N-linked glycosylation.
30905720	3	100	theme	forms	701:705	arg1	two					688:690	two	688:690	two	688:690	Three different molecular forms of PLA1 were identified as allergenic proteins, and two of these forms were modified by N-linked glycosylation.
30905720	4	101	theme	proteins	815:822	arg1	proteins					815:822	hitherto undescribed proteins	794:822	hitherto undescribed proteins	794:822	This study reveals an extensive repertoire of hitherto undescribed proteins that were classified into the following six different functional groups: (i) typical venom proteins; (ii) proteins related to the folding/conformation and PTMs of toxins; (iii) proteins that protect toxins from oxidative stress; (iv) proteins involved in chemical communication; (v) housekeeping proteins; and (vi) uncharacterized proteins.
30905720	4	101	theme	proteins	815:822	arg1	repertoire					780:789	an extensive repertoire	767:789	an extensive repertoire of hitherto undescribed proteins that were classified into the following six different functional groups: (i) typical venom proteins; (ii) proteins related to the folding/conformation and PTMs of toxins; (iii) proteins that protect toxins from oxidative stress	767:1050	This study reveals an extensive repertoire of hitherto undescribed proteins that were classified into the following six different functional groups: (i) typical venom proteins; (ii) proteins related to the folding/conformation and PTMs of toxins; (iii) proteins that protect toxins from oxidative stress; (iv) proteins involved in chemical communication; (v) housekeeping proteins; and (vi) uncharacterized proteins.
29324290	5	0	theme	decreased	936:944	arg1	deposition					950:959	decreased MBL deposition	936:959	decreased MBL deposition	936:959	Instead, the reduced disease induced by glycan-deficient viruses was linked to decreased MBL deposition and complement activation within inflamed tissues.
29324290	1	1	theme	Ross	241:244	arg1	virus					252:256	Ross River virus	241:256	Ross River virus	241:256	Mannose binding lectin (MBL) generally plays a protective role during viral infection, yet MBL-mediated complement activation promotes Ross River virus (RRV)-induced inflammatory tissue destruction, contributing to arthritis and myositis.
29324290	6	2	theme	MBL	1078:1080	arg1	deposition					1082:1091	MBL deposition	1078:1091	MBL deposition	1078:1091	These results demonstrate that the viral N-linked glycans promote MBL deposition and complement activation onto RRV-infected cells, contributing to the development of RRV-induced myositis.
29324290	1	3	theme	River	246:250	arg1	virus					252:256	Ross River virus	241:256	Ross River virus	241:256	Mannose binding lectin (MBL) generally plays a protective role during viral infection, yet MBL-mediated complement activation promotes Ross River virus (RRV)-induced inflammatory tissue destruction, contributing to arthritis and myositis.
29324290	0	4	theme	complement	87:96	arg1	system					98:103	the host complement system	78:103	the host complement system	78:103	Ross River virus envelope glycans contribute to disease through activation of the host complement system.
29324290	5	5	theme	complement	965:974	arg1	activation					976:985	complement activation	965:985	complement activation	965:985	Instead, the reduced disease induced by glycan-deficient viruses was linked to decreased MBL deposition and complement activation within inflamed tissues.
29324290	4	6	theme	viral	789:793	arg1	load					795:798	similar viral load	781:798	similar viral load	781:798	Moreover, the glycan-deficient viruses exhibited reduced disease and tissue damage in a mouse model of RRV-induced myositis compared to wild-type RRV, despite similar viral load and inflammatory infiltrates within the skeletal muscle.
29324290	6	7	theme	myositis	1191:1198	arg1	development					1164:1174	the development	1160:1174	the development of RRV-induced myositis	1160:1198	These results demonstrate that the viral N-linked glycans promote MBL deposition and complement activation onto RRV-infected cells, contributing to the development of RRV-induced myositis.
29324290	0	8	theme	host	82:85	arg1	system					98:103	the host complement system	78:103	the host complement system	78:103	Ross River virus envelope glycans contribute to disease through activation of the host complement system.
29324290	4	9	theme	skeletal	840:847	arg1	muscle					849:854	the skeletal muscle	836:854	the skeletal muscle	836:854	Moreover, the glycan-deficient viruses exhibited reduced disease and tissue damage in a mouse model of RRV-induced myositis compared to wild-type RRV, despite similar viral load and inflammatory infiltrates within the skeletal muscle.
29324290	3	10	theme	envelope	513:520	arg1	glycans					531:537	the envelope N-linked glycans	509:537	the envelope N-linked glycans	509:537	Using a panel of RRV mutants lacking the envelope N-linked glycans, we found that MBL deposition onto infected cells was dependent on the E2 glycans.
29324290	5	11	theme	reduced	870:876	arg1	disease					878:884	the reduced disease	866:884	the reduced disease induced by glycan-deficient viruses	866:920	Instead, the reduced disease induced by glycan-deficient viruses was linked to decreased MBL deposition and complement activation within inflamed tissues.
29324290	3	12	theme	mutants	493:499	arg1	panel					480:484	a panel	478:484	a panel of RRV mutants lacking the envelope N-linked glycans	478:537	Using a panel of RRV mutants lacking the envelope N-linked glycans, we found that MBL deposition onto infected cells was dependent on the E2 glycans.
29324290	6	13	theme	RRV-infected	1124:1135	arg1	cells					1137:1141	RRV-infected cells	1124:1141	RRV-infected cells	1124:1141	These results demonstrate that the viral N-linked glycans promote MBL deposition and complement activation onto RRV-infected cells, contributing to the development of RRV-induced myositis.
29324290	0	14	theme	system	98:103	arg1	activation					64:73	activation	64:73	activation of the host complement system	64:103	Ross River virus envelope glycans contribute to disease through activation of the host complement system.
29324290	2	15	theme	RRV	421:423	arg1	glycoproteins					434:446	the RRV envelope glycoproteins	417:446	the RRV envelope glycoproteins	417:446	As MBL binds to carbohydrates, we hypothesized that N-linked glycans on the RRV envelope glycoproteins act as ligands for MBL.
29324290	1	16	theme	protective	153:162	arg1	role					164:167	a protective role	151:167	a protective role	151:167	Mannose binding lectin (MBL) generally plays a protective role during viral infection, yet MBL-mediated complement activation promotes Ross River virus (RRV)-induced inflammatory tissue destruction, contributing to arthritis and myositis.
29324290	0	17	theme	River	5:9	arg1	virus					11:15	Ross River virus	0:15	Ross River virus envelope glycans	0:32	Ross River virus envelope glycans contribute to disease through activation of the host complement system.
29324290	5	18	theme	glycan-deficient	897:912	arg1	viruses					914:920	glycan-deficient viruses	897:920	glycan-deficient viruses	897:920	Instead, the reduced disease induced by glycan-deficient viruses was linked to decreased MBL deposition and complement activation within inflamed tissues.
29324290	6	19	theme	N-linked	1053:1060	arg1	glycans					1062:1068	the viral N-linked glycans	1043:1068	the viral N-linked glycans	1043:1068	These results demonstrate that the viral N-linked glycans promote MBL deposition and complement activation onto RRV-infected cells, contributing to the development of RRV-induced myositis.
29324290	0	20	theme	Ross	0:3	arg1	virus					11:15	Ross River virus	0:15	Ross River virus envelope glycans	0:32	Ross River virus envelope glycans contribute to disease through activation of the host complement system.
29324290	4	21	theme	inflammatory	804:815	arg1	infiltrates					817:827	inflammatory infiltrates	804:827	inflammatory infiltrates within the skeletal muscle	804:854	Moreover, the glycan-deficient viruses exhibited reduced disease and tissue damage in a mouse model of RRV-induced myositis compared to wild-type RRV, despite similar viral load and inflammatory infiltrates within the skeletal muscle.
29324290	6	22	theme	viral	1047:1051	arg1	glycans					1062:1068	the viral N-linked glycans	1043:1068	the viral N-linked glycans	1043:1068	These results demonstrate that the viral N-linked glycans promote MBL deposition and complement activation onto RRV-infected cells, contributing to the development of RRV-induced myositis.
29324290	0	23	theme	envelope	17:24	arg1	glycans					26:32	Ross River virus envelope glycans	0:32	Ross River virus envelope glycans	0:32	Ross River virus envelope glycans contribute to disease through activation of the host complement system.
29324290	3	24	theme	N-linked	522:529	arg1	glycans					531:537	the envelope N-linked glycans	509:537	the envelope N-linked glycans	509:537	Using a panel of RRV mutants lacking the envelope N-linked glycans, we found that MBL deposition onto infected cells was dependent on the E2 glycans.
29324290	5	25	theme	inflamed	994:1001	arg1	tissues					1003:1009	inflamed tissues	994:1009	inflamed tissues	994:1009	Instead, the reduced disease induced by glycan-deficient viruses was linked to decreased MBL deposition and complement activation within inflamed tissues.
29324290	1	26	theme	viral	176:180	arg1	infection					182:190	viral infection	176:190	viral infection	176:190	Mannose binding lectin (MBL) generally plays a protective role during viral infection, yet MBL-mediated complement activation promotes Ross River virus (RRV)-induced inflammatory tissue destruction, contributing to arthritis and myositis.
29324290	0	27	theme	virus	11:15	arg1	glycans					26:32	Ross River virus envelope glycans	0:32	Ross River virus envelope glycans	0:32	Ross River virus envelope glycans contribute to disease through activation of the host complement system.
29324290	1	28	theme	-induced	263:270	arg1	destruction					292:302	Ross River virus (RRV)-induced inflammatory tissue destruction	241:302	Ross River virus (RRV)-induced inflammatory tissue destruction	241:302	Mannose binding lectin (MBL) generally plays a protective role during viral infection, yet MBL-mediated complement activation promotes Ross River virus (RRV)-induced inflammatory tissue destruction, contributing to arthritis and myositis.
29324290	4	29	theme	myositis	737:744	arg1	model					716:720	a mouse model	708:720	a mouse model of RRV-induced myositis	708:744	Moreover, the glycan-deficient viruses exhibited reduced disease and tissue damage in a mouse model of RRV-induced myositis compared to wild-type RRV, despite similar viral load and inflammatory infiltrates within the skeletal muscle.
29324290	2	30	theme	N-linked	397:404	arg1	ligands					455:461	ligands	455:461	ligands for MBL	455:469	As MBL binds to carbohydrates, we hypothesized that N-linked glycans on the RRV envelope glycoproteins act as ligands for MBL.
29324290	2	30	theme	N-linked	397:404	arg1	glycans					406:412	N-linked glycans	397:412	N-linked glycans on the RRV envelope glycoproteins	397:446	As MBL binds to carbohydrates, we hypothesized that N-linked glycans on the RRV envelope glycoproteins act as ligands for MBL.
29324290	1	31	theme	inflammatory	272:283	arg1	destruction					292:302	Ross River virus (RRV)-induced inflammatory tissue destruction	241:302	Ross River virus (RRV)-induced inflammatory tissue destruction	241:302	Mannose binding lectin (MBL) generally plays a protective role during viral infection, yet MBL-mediated complement activation promotes Ross River virus (RRV)-induced inflammatory tissue destruction, contributing to arthritis and myositis.
29324290	3	32	theme	E2	610:611	arg1	glycans					613:619	the E2 glycans	606:619	the E2 glycans	606:619	Using a panel of RRV mutants lacking the envelope N-linked glycans, we found that MBL deposition onto infected cells was dependent on the E2 glycans.
29324290	3	33	theme	MBL	554:556	arg1	deposition					558:567	MBL deposition	554:567	MBL deposition onto infected cells	554:587	Using a panel of RRV mutants lacking the envelope N-linked glycans, we found that MBL deposition onto infected cells was dependent on the E2 glycans.
29324290	1	34	theme	tissue	285:290	arg1	destruction					292:302	Ross River virus (RRV)-induced inflammatory tissue destruction	241:302	Ross River virus (RRV)-induced inflammatory tissue destruction	241:302	Mannose binding lectin (MBL) generally plays a protective role during viral infection, yet MBL-mediated complement activation promotes Ross River virus (RRV)-induced inflammatory tissue destruction, contributing to arthritis and myositis.
29324290	6	35	theme	RRV-induced	1179:1189	arg1	myositis					1191:1198	RRV-induced myositis	1179:1198	RRV-induced myositis	1179:1198	These results demonstrate that the viral N-linked glycans promote MBL deposition and complement activation onto RRV-infected cells, contributing to the development of RRV-induced myositis.
29324290	2	36	gly	glycoproteins	434:446	arg1	glycoproteins					434:446	the RRV envelope glycoproteins	417:446	the RRV envelope glycoproteins	417:446	As MBL binds to carbohydrates, we hypothesized that N-linked glycans on the RRV envelope glycoproteins act as ligands for MBL.
29324290	6	37	theme	complement	1097:1106	arg1	activation					1108:1117	complement activation	1097:1117	complement activation	1097:1117	These results demonstrate that the viral N-linked glycans promote MBL deposition and complement activation onto RRV-infected cells, contributing to the development of RRV-induced myositis.
29324290	1	38	theme	Mannose	106:112	arg1	lectin					122:127	Mannose binding lectin	106:127	Mannose binding lectin (MBL)	106:133	Mannose binding lectin (MBL) generally plays a protective role during viral infection, yet MBL-mediated complement activation promotes Ross River virus (RRV)-induced inflammatory tissue destruction, contributing to arthritis and myositis.
29324290	1	38	theme	Mannose	106:112	arg1	MBL					130:132	MBL	130:132	MBL	130:132	Mannose binding lectin (MBL) generally plays a protective role during viral infection, yet MBL-mediated complement activation promotes Ross River virus (RRV)-induced inflammatory tissue destruction, contributing to arthritis and myositis.
29324290	5	39	attach	linked	926:931	arg1	activation					976:985	complement activation	965:985	complement activation	965:985	Instead, the reduced disease induced by glycan-deficient viruses was linked to decreased MBL deposition and complement activation within inflamed tissues.
29324290	5	39	attach	linked	926:931	arg2	disease					878:884	the reduced disease	866:884	the reduced disease induced by glycan-deficient viruses	866:920	Instead, the reduced disease induced by glycan-deficient viruses was linked to decreased MBL deposition and complement activation within inflamed tissues.
29324290	5	39	attach	linked	926:931	arg1	deposition					950:959	decreased MBL deposition	936:959	decreased MBL deposition	936:959	Instead, the reduced disease induced by glycan-deficient viruses was linked to decreased MBL deposition and complement activation within inflamed tissues.
29324290	6	40	link	N-linked	1053:1060	arg1	glycans					1062:1068	the viral N-linked glycans	1043:1068	the viral N-linked glycans	1043:1068	These results demonstrate that the viral N-linked glycans promote MBL deposition and complement activation onto RRV-infected cells, contributing to the development of RRV-induced myositis.
29324290	4	41	theme	similar	781:787	arg1	load					795:798	similar viral load	781:798	similar viral load	781:798	Moreover, the glycan-deficient viruses exhibited reduced disease and tissue damage in a mouse model of RRV-induced myositis compared to wild-type RRV, despite similar viral load and inflammatory infiltrates within the skeletal muscle.
29324290	1	42	theme	binding	114:120	arg1	lectin					122:127	Mannose binding lectin	106:127	Mannose binding lectin (MBL)	106:133	Mannose binding lectin (MBL) generally plays a protective role during viral infection, yet MBL-mediated complement activation promotes Ross River virus (RRV)-induced inflammatory tissue destruction, contributing to arthritis and myositis.
29324290	1	42	theme	binding	114:120	arg1	MBL					130:132	MBL	130:132	MBL	130:132	Mannose binding lectin (MBL) generally plays a protective role during viral infection, yet MBL-mediated complement activation promotes Ross River virus (RRV)-induced inflammatory tissue destruction, contributing to arthritis and myositis.
29324290	4	43	theme	wild-type	758:766	arg1	RRV					768:770	wild-type RRV	758:770	wild-type RRV	758:770	Moreover, the glycan-deficient viruses exhibited reduced disease and tissue damage in a mouse model of RRV-induced myositis compared to wild-type RRV, despite similar viral load and inflammatory infiltrates within the skeletal muscle.
29324290	4	44	theme	tissue	691:696	arg1	damage					698:703	tissue damage	691:703	tissue damage	691:703	Moreover, the glycan-deficient viruses exhibited reduced disease and tissue damage in a mouse model of RRV-induced myositis compared to wild-type RRV, despite similar viral load and inflammatory infiltrates within the skeletal muscle.
29324290	4	45	theme	mouse	710:714	arg1	model					716:720	a mouse model	708:720	a mouse model of RRV-induced myositis	708:744	Moreover, the glycan-deficient viruses exhibited reduced disease and tissue damage in a mouse model of RRV-induced myositis compared to wild-type RRV, despite similar viral load and inflammatory infiltrates within the skeletal muscle.
29324290	1	46	theme	MBL-mediated	197:208	arg1	activation					221:230	MBL-mediated complement activation	197:230	MBL-mediated complement activation	197:230	Mannose binding lectin (MBL) generally plays a protective role during viral infection, yet MBL-mediated complement activation promotes Ross River virus (RRV)-induced inflammatory tissue destruction, contributing to arthritis and myositis.
29324290	4	47	theme	RRV-induced	725:735	arg1	myositis					737:744	RRV-induced myositis	725:744	RRV-induced myositis	725:744	Moreover, the glycan-deficient viruses exhibited reduced disease and tissue damage in a mouse model of RRV-induced myositis compared to wild-type RRV, despite similar viral load and inflammatory infiltrates within the skeletal muscle.
29324290	5	48	theme	MBL	946:948	arg1	deposition					950:959	decreased MBL deposition	936:959	decreased MBL deposition	936:959	Instead, the reduced disease induced by glycan-deficient viruses was linked to decreased MBL deposition and complement activation within inflamed tissues.
29324290	3	49	theme	RRV	489:491	arg1	mutants					493:499	RRV mutants	489:499	RRV mutants lacking the envelope N-linked glycans	489:537	Using a panel of RRV mutants lacking the envelope N-linked glycans, we found that MBL deposition onto infected cells was dependent on the E2 glycans.
29324290	1	50	theme	complement	210:219	arg1	activation					221:230	MBL-mediated complement activation	197:230	MBL-mediated complement activation	197:230	Mannose binding lectin (MBL) generally plays a protective role during viral infection, yet MBL-mediated complement activation promotes Ross River virus (RRV)-induced inflammatory tissue destruction, contributing to arthritis and myositis.
29324290	3	51	link	N-linked	522:529	arg1	glycans					531:537	the envelope N-linked glycans	509:537	the envelope N-linked glycans	509:537	Using a panel of RRV mutants lacking the envelope N-linked glycans, we found that MBL deposition onto infected cells was dependent on the E2 glycans.
29324290	2	52	link	N-linked	397:404	arg1	ligands					455:461	ligands	455:461	ligands for MBL	455:469	As MBL binds to carbohydrates, we hypothesized that N-linked glycans on the RRV envelope glycoproteins act as ligands for MBL.
29324290	2	52	link	N-linked	397:404	arg1	glycans					406:412	N-linked glycans	397:412	N-linked glycans on the RRV envelope glycoproteins	397:446	As MBL binds to carbohydrates, we hypothesized that N-linked glycans on the RRV envelope glycoproteins act as ligands for MBL.
29324290	4	53	theme	glycan-deficient	636:651	arg1	viruses					653:659	the glycan-deficient viruses	632:659	the glycan-deficient viruses	632:659	Moreover, the glycan-deficient viruses exhibited reduced disease and tissue damage in a mouse model of RRV-induced myositis compared to wild-type RRV, despite similar viral load and inflammatory infiltrates within the skeletal muscle.
29324290	1	54	dep	-induced	263:270	arg1	virus					252:256	Ross River virus	241:256	Ross River virus	241:256	Mannose binding lectin (MBL) generally plays a protective role during viral infection, yet MBL-mediated complement activation promotes Ross River virus (RRV)-induced inflammatory tissue destruction, contributing to arthritis and myositis.
29324290	3	55	theme	infected	574:581	arg1	cells					583:587	infected cells	574:587	infected cells	574:587	Using a panel of RRV mutants lacking the envelope N-linked glycans, we found that MBL deposition onto infected cells was dependent on the E2 glycans.
29324290	2	56	from	glycans	406:412	arg1	glycoproteins					434:446	the RRV envelope glycoproteins	417:446	the RRV envelope glycoproteins	417:446	As MBL binds to carbohydrates, we hypothesized that N-linked glycans on the RRV envelope glycoproteins act as ligands for MBL.
29324290	2	57	theme	envelope	425:432	arg1	glycoproteins					434:446	the RRV envelope glycoproteins	417:446	the RRV envelope glycoproteins	417:446	As MBL binds to carbohydrates, we hypothesized that N-linked glycans on the RRV envelope glycoproteins act as ligands for MBL.
29324290	4	58	theme	reduced	671:677	arg1	disease					679:685	reduced disease	671:685	reduced disease	671:685	Moreover, the glycan-deficient viruses exhibited reduced disease and tissue damage in a mouse model of RRV-induced myositis compared to wild-type RRV, despite similar viral load and inflammatory infiltrates within the skeletal muscle.
31680955	2	0	theme	Triple-negative	341:355	arg1	TNBC					372:375	TNBC	372:375	TNBC	372:375	Triple-negative breast cancer (TNBC) is associated with a severely poor prognosis due to its invasive phenotype and lack of biomarker-driven-targeted therapies.
31680955	2	0	theme	Triple-negative	341:355	arg1	cancer					364:369	Triple-negative breast cancer	341:369	Triple-negative breast cancer (TNBC)	341:376	Triple-negative breast cancer (TNBC) is associated with a severely poor prognosis due to its invasive phenotype and lack of biomarker-driven-targeted therapies.
31680955	14	1	theme	rational	2273:2280	arg1	approach					2282:2289	a rational approach	2271:2289	a rational approach to discovering compounds with a polypharmacology-based therapeutic value	2271:2362	Conclusion: This study provides a rational approach to discovering compounds with a polypharmacology-based therapeutic value.
31680955	1	2	theme	cancers	268:274	arg1	variety					257:263	a variety	255:263	a variety of cancers	255:274	Background: Studies have shown that the natural products of Astragalus membranaceus (AM) can effectively interfere with a variety of cancers, but their mechanism of action on breast cancer remains unclear.
31680955	1	2	theme	cancers	268:274	arg1	cancers					268:274	cancers	268:274	cancers	268:274	Background: Studies have shown that the natural products of Astragalus membranaceus (AM) can effectively interfere with a variety of cancers, but their mechanism of action on breast cancer remains unclear.
31680955	12	3	theme	component	1921:1929	arg1	APS					1931:1933	the key component APS	1913:1933	the key component APS	1913:1933	Finally, the key component APS and biomarkers PIK3CG, AKT, and BCL2 were identified.
31680955	9	4	theme	signaling	1625:1633	arg1	pathways					1635:1642	correlated signaling pathways	1614:1642	correlated signaling pathways	1614:1642	We also performed western blot analysis of key proteins to probe the mechanisms of correlated signaling pathways.
31680955	1	5	from	mechanism	287:295	arg1	cancer					317:322	breast cancer	310:322	breast cancer	310:322	Background: Studies have shown that the natural products of Astragalus membranaceus (AM) can effectively interfere with a variety of cancers, but their mechanism of action on breast cancer remains unclear.
31680955	3	6	theme	potential	521:529	arg1	mechanism					531:539	the potential mechanism	517:539	the potential mechanism of the target composition acting on TNBC	517:580	In this study, the potential mechanism of the target composition acting on TNBC was explored by integrated pharmacological models and in vitro experiments.
31680955	6	7	theme	accurate	1220:1227	arg1	models					1241:1246	accurate combination models	1220:1246	accurate combination models	1220:1246	Molecular docking was used to evaluate the affinity between compounds and targets and obtain accurate combination models.
31680955	1	8	theme	Astragalus	195:204	arg1	AM					220:221	AM	220:221	AM	220:221	Background: Studies have shown that the natural products of Astragalus membranaceus (AM) can effectively interfere with a variety of cancers, but their mechanism of action on breast cancer remains unclear.
31680955	1	8	theme	Astragalus	195:204	arg1	membranaceus					206:217	Astragalus membranaceus	195:217	Astragalus membranaceus (AM)	195:222	Background: Studies have shown that the natural products of Astragalus membranaceus (AM) can effectively interfere with a variety of cancers, but their mechanism of action on breast cancer remains unclear.
31680955	11	9	theme	key	1879:1881	arg1	hubs					1883:1886	key hubs	1879:1886	key hubs of the network	1879:1901	Topology analysis and molecular docking were used as secondary screens to identify key hubs of the network.
31680955	8	10	used	used	1404:1407	arg2	assay					1347:1351	The cell counting kit-8 (CCK-8) assay	1315:1351	The cell counting kit-8 (CCK-8) assay	1315:1351	The cell counting kit-8 (CCK-8) assay, invasion assay, and flow cytometric analysis were used to assess cell viability, invasiveness, and apoptosis, respectively, after Astragalus polysaccharides (APS) intervention.
31680955	8	10	used	used	1404:1407	arg2	analysis					1390:1397	flow cytometric analysis	1374:1397	flow cytometric analysis	1374:1397	The cell counting kit-8 (CCK-8) assay, invasion assay, and flow cytometric analysis were used to assess cell viability, invasiveness, and apoptosis, respectively, after Astragalus polysaccharides (APS) intervention.
31680955	8	10	used	used	1404:1407	arg2	assay					1363:1367	invasion assay	1354:1367	invasion assay	1354:1367	The cell counting kit-8 (CCK-8) assay, invasion assay, and flow cytometric analysis were used to assess cell viability, invasiveness, and apoptosis, respectively, after Astragalus polysaccharides (APS) intervention.
31680955	10	11	theme	compound-target	1670:1684	arg1	"					1685:1685	"compound-target"	1669:1685	"compound-target" (339 nodes and 695 edges)	1669:1711	Results: We constructed "compound-target" (339 nodes and 695 edges) and "compound-disease" (414 nodes and 6458 edges) networks using interaction data.
31680955	15	12	theme	promising	2515:2523	arg1	strategy					2537:2544	a promising therapeutic strategy	2513:2544	a promising therapeutic strategy for TNBC	2513:2553	Our data established that APS intervenes with TNBC cell invasion, proliferation, and apoptosis via the PIK3CG/AKT/BCL2 pathway and could thus offer a promising therapeutic strategy for TNBC.
31680955	4	13	theme	multiattribute	899:912	arg1	analysis					914:921	multiattribute analysis	899:921	multiattribute analysis	899:921	Materials and Methods: Based on the Gene Expression Omnibus (GEO) database and the relational database of Traditional Chinese Medicines (TCMs), the drug and target components were initially screened to construct a common network module, and multiattribute analysis was then used to characterize the network and obtain key drug-target information.
31680955	10	14	theme	414	1737:1739	arg1	nodes					1741:1745	414 nodes	1737:1745	414 nodes	1737:1745	Results: We constructed "compound-target" (339 nodes and 695 edges) and "compound-disease" (414 nodes and 6458 edges) networks using interaction data.
31680955	4	15	theme	Expression	699:708	arg1	database					724:731	the Gene Expression Omnibus (GEO) database	690:731	the Gene Expression Omnibus (GEO) database	690:731	Materials and Methods: Based on the Gene Expression Omnibus (GEO) database and the relational database of Traditional Chinese Medicines (TCMs), the drug and target components were initially screened to construct a common network module, and multiattribute analysis was then used to characterize the network and obtain key drug-target information.
31680955	5	16	theme	network	1018:1024	arg1	analysis					1035:1042	network topology analysis	1018:1042	network topology analysis	1018:1042	Furthermore, network topology analysis was used to characterize the betweenness and closeness of key hubs in the network.
31680955	4	17	used	used	932:935	arg2	analysis					914:921	multiattribute analysis	899:921	multiattribute analysis	899:921	Materials and Methods: Based on the Gene Expression Omnibus (GEO) database and the relational database of Traditional Chinese Medicines (TCMs), the drug and target components were initially screened to construct a common network module, and multiattribute analysis was then used to characterize the network and obtain key drug-target information.
31680955	4	18	theme	network	879:885	arg1	module					887:892	a common network module	870:892	a common network module	870:892	Materials and Methods: Based on the Gene Expression Omnibus (GEO) database and the relational database of Traditional Chinese Medicines (TCMs), the drug and target components were initially screened to construct a common network module, and multiattribute analysis was then used to characterize the network and obtain key drug-target information.
31680955	0	19	theme	Triple-Negative	66:80	arg1	Cancer					89:94	Triple-Negative Breast Cancer	66:94	Triple-Negative Breast Cancer	66:94	The Modulatory Properties of Astragalus membranaceus Treatment on Triple-Negative Breast Cancer: An Integrated Pharmacological Method.
31680955	5	20	theme	key	1102:1104	arg1	hubs					1106:1109	key hubs	1102:1109	key hubs	1102:1109	Furthermore, network topology analysis was used to characterize the betweenness and closeness of key hubs in the network.
31680955	3	21	theme	integrated	598:607	arg1	models					625:630	integrated pharmacological models	598:630	integrated pharmacological models	598:630	In this study, the potential mechanism of the target composition acting on TNBC was explored by integrated pharmacological models and in vitro experiments.
31680955	2	22	theme	biomarker-driven-targeted	465:489	arg1	therapies					491:499	biomarker-driven-targeted therapies	465:499	biomarker-driven-targeted therapies	465:499	Triple-negative breast cancer (TNBC) is associated with a severely poor prognosis due to its invasive phenotype and lack of biomarker-driven-targeted therapies.
31680955	0	23	from	Properties	15:24	arg1	Cancer					89:94	Triple-Negative Breast Cancer	66:94	Triple-Negative Breast Cancer	66:94	The Modulatory Properties of Astragalus membranaceus Treatment on Triple-Negative Breast Cancer: An Integrated Pharmacological Method.
31680955	13	24	theme	TNBC	2066:2069	arg1	activity					2076:2083	TNBC cell activity	2066:2083	TNBC cell activity	2066:2083	The in vitro experimental results confirmed that APS can effectively inhibit TNBC cell activity, reduce invasion, promote apoptosis, and then counteract TNBC symptoms in a dose-dependent manner, most likely by inhibiting the PIK3CG/AKT/BCL2 pathway.
31680955	8	25	theme	counting	1324:1331	arg1	kit-8					1333:1337	The cell counting kit-8	1315:1337	The cell counting kit-8 (CCK-8) assay	1315:1351	The cell counting kit-8 (CCK-8) assay, invasion assay, and flow cytometric analysis were used to assess cell viability, invasiveness, and apoptosis, respectively, after Astragalus polysaccharides (APS) intervention.
31680955	8	25	theme	counting	1324:1331	arg1	CCK-8					1340:1344	CCK-8	1340:1344	CCK-8	1340:1344	The cell counting kit-8 (CCK-8) assay, invasion assay, and flow cytometric analysis were used to assess cell viability, invasiveness, and apoptosis, respectively, after Astragalus polysaccharides (APS) intervention.
31680955	14	26	with	compounds	2306:2314	arg1	value					2358:2362	a polypharmacology-based therapeutic value	2321:2362	a polypharmacology-based therapeutic value	2321:2362	Conclusion: This study provides a rational approach to discovering compounds with a polypharmacology-based therapeutic value.
31680955	0	27	theme	Pharmacological	111:125	arg1	Method					127:132	An Integrated Pharmacological Method	97:132	An Integrated Pharmacological Method	97:132	The Modulatory Properties of Astragalus membranaceus Treatment on Triple-Negative Breast Cancer: An Integrated Pharmacological Method.
31680955	8	28	theme	Astragalus	1484:1493	arg1	APS					1512:1514	APS	1512:1514	APS	1512:1514	The cell counting kit-8 (CCK-8) assay, invasion assay, and flow cytometric analysis were used to assess cell viability, invasiveness, and apoptosis, respectively, after Astragalus polysaccharides (APS) intervention.
31680955	8	28	theme	Astragalus	1484:1493	arg1	polysaccharides					1495:1509	Astragalus polysaccharides	1484:1509	Astragalus polysaccharides (APS) intervention	1484:1528	The cell counting kit-8 (CCK-8) assay, invasion assay, and flow cytometric analysis were used to assess cell viability, invasiveness, and apoptosis, respectively, after Astragalus polysaccharides (APS) intervention.
31680955	11	29	theme	molecular	1818:1826	arg1	analysis					1805:1812	Topology analysis	1796:1812	Topology analysis	1796:1812	Topology analysis and molecular docking were used as secondary screens to identify key hubs of the network.
31680955	11	29	theme	molecular	1818:1826	arg1	screens					1859:1865	secondary screens	1849:1865	secondary screens	1849:1865	Topology analysis and molecular docking were used as secondary screens to identify key hubs of the network.
31680955	11	29	theme	molecular	1818:1826	arg1	docking					1828:1834	molecular docking	1818:1834	molecular docking	1818:1834	Topology analysis and molecular docking were used as secondary screens to identify key hubs of the network.
31680955	15	30	theme	cell	2416:2419	arg1	invasion					2421:2428	TNBC cell invasion	2411:2428	TNBC cell invasion	2411:2428	Our data established that APS intervenes with TNBC cell invasion, proliferation, and apoptosis via the PIK3CG/AKT/BCL2 pathway and could thus offer a promising therapeutic strategy for TNBC.
31680955	2	31	theme	due	423:425	arg1	prognosis					413:421	a severely poor prognosis	397:421	a severely poor prognosis due to its invasive phenotype and lack of biomarker-driven-targeted therapies	397:499	Triple-negative breast cancer (TNBC) is associated with a severely poor prognosis due to its invasive phenotype and lack of biomarker-driven-targeted therapies.
31680955	2	32	theme	poor	408:411	arg1	prognosis					413:421	a severely poor prognosis	397:421	a severely poor prognosis due to its invasive phenotype and lack of biomarker-driven-targeted therapies	397:499	Triple-negative breast cancer (TNBC) is associated with a severely poor prognosis due to its invasive phenotype and lack of biomarker-driven-targeted therapies.
31680955	7	33	theme	key	1292:1294	arg1	targets					1306:1312	the key component targets	1288:1312	the key component targets	1288:1312	Finally, in vitro experiments verified the key component targets.
31680955	10	34	theme	interaction	1778:1788	arg1	data					1790:1793	interaction data	1778:1793	interaction data	1778:1793	Results: We constructed "compound-target" (339 nodes and 695 edges) and "compound-disease" (414 nodes and 6458 edges) networks using interaction data.
31680955	0	35	theme	Astragalus	29:38	arg1	Treatment					53:61	Astragalus membranaceus Treatment	29:61	Astragalus membranaceus Treatment	29:61	The Modulatory Properties of Astragalus membranaceus Treatment on Triple-Negative Breast Cancer: An Integrated Pharmacological Method.
31680955	9	36	theme	blot	1557:1560	arg1	analysis					1562:1569	western blot analysis	1549:1569	western blot analysis of key proteins	1549:1585	We also performed western blot analysis of key proteins to probe the mechanisms of correlated signaling pathways.
31680955	12	37	theme	key	1917:1919	arg1	APS					1931:1933	the key component APS	1913:1933	the key component APS	1913:1933	Finally, the key component APS and biomarkers PIK3CG, AKT, and BCL2 were identified.
31680955	4	38	theme	drug-target	980:990	arg1	information					992:1002	key drug-target information	976:1002	key drug-target information	976:1002	Materials and Methods: Based on the Gene Expression Omnibus (GEO) database and the relational database of Traditional Chinese Medicines (TCMs), the drug and target components were initially screened to construct a common network module, and multiattribute analysis was then used to characterize the network and obtain key drug-target information.
31680955	3	39	theme	in	636:637	arg1	experiments					645:655	in vitro experiments	636:655	in vitro experiments	636:655	In this study, the potential mechanism of the target composition acting on TNBC was explored by integrated pharmacological models and in vitro experiments.
31680955	0	40	theme	Treatment	53:61	arg1	Properties					15:24	The Modulatory Properties	0:24	The Modulatory Properties of Astragalus membranaceus Treatment on Triple-Negative Breast Cancer	0:94	The Modulatory Properties of Astragalus membranaceus Treatment on Triple-Negative Breast Cancer: An Integrated Pharmacological Method.
31680955	4	41	theme	drug	806:809	arg1	components					822:831	the drug and target components	802:831	components	822:831	Materials and Methods: Based on the Gene Expression Omnibus (GEO) database and the relational database of Traditional Chinese Medicines (TCMs), the drug and target components were initially screened to construct a common network module, and multiattribute analysis was then used to characterize the network and obtain key drug-target information.
31680955	11	42	used	used	1841:1844	arg2	analysis					1805:1812	Topology analysis	1796:1812	Topology analysis	1796:1812	Topology analysis and molecular docking were used as secondary screens to identify key hubs of the network.
31680955	11	42	used	used	1841:1844	arg2	screens					1859:1865	secondary screens	1849:1865	secondary screens	1849:1865	Topology analysis and molecular docking were used as secondary screens to identify key hubs of the network.
31680955	11	42	used	used	1841:1844	arg2	docking					1828:1834	molecular docking	1818:1834	molecular docking	1818:1834	Topology analysis and molecular docking were used as secondary screens to identify key hubs of the network.
31680955	11	43	theme	network	1895:1901	arg1	hubs					1883:1886	key hubs	1879:1886	key hubs of the network	1879:1901	Topology analysis and molecular docking were used as secondary screens to identify key hubs of the network.
31680955	8	44	theme	cytometric	1379:1388	arg1	analysis					1390:1397	flow cytometric analysis	1374:1397	flow cytometric analysis	1374:1397	The cell counting kit-8 (CCK-8) assay, invasion assay, and flow cytometric analysis were used to assess cell viability, invasiveness, and apoptosis, respectively, after Astragalus polysaccharides (APS) intervention.
31680955	13	45	theme	in	1993:1994	arg1	results					2015:2021	The in vitro experimental results	1989:2021	The in vitro experimental results	1989:2021	The in vitro experimental results confirmed that APS can effectively inhibit TNBC cell activity, reduce invasion, promote apoptosis, and then counteract TNBC symptoms in a dose-dependent manner, most likely by inhibiting the PIK3CG/AKT/BCL2 pathway.
31680955	13	46	theme	cell	2071:2074	arg1	activity					2076:2083	TNBC cell activity	2066:2083	TNBC cell activity	2066:2083	The in vitro experimental results confirmed that APS can effectively inhibit TNBC cell activity, reduce invasion, promote apoptosis, and then counteract TNBC symptoms in a dose-dependent manner, most likely by inhibiting the PIK3CG/AKT/BCL2 pathway.
31680955	9	47	theme	proteins	1578:1585	arg1	analysis					1562:1569	western blot analysis	1549:1569	western blot analysis of key proteins	1549:1585	We also performed western blot analysis of key proteins to probe the mechanisms of correlated signaling pathways.
31680955	14	48	theme	discovering	2294:2304	arg1	compounds					2306:2314	discovering compounds	2294:2314	discovering compounds with a polypharmacology-based therapeutic value	2294:2362	Conclusion: This study provides a rational approach to discovering compounds with a polypharmacology-based therapeutic value.
31680955	2	49	theme	breast	357:362	arg1	TNBC					372:375	TNBC	372:375	TNBC	372:375	Triple-negative breast cancer (TNBC) is associated with a severely poor prognosis due to its invasive phenotype and lack of biomarker-driven-targeted therapies.
31680955	2	49	theme	breast	357:362	arg1	cancer					364:369	Triple-negative breast cancer	341:369	Triple-negative breast cancer (TNBC)	341:376	Triple-negative breast cancer (TNBC) is associated with a severely poor prognosis due to its invasive phenotype and lack of biomarker-driven-targeted therapies.
31680955	15	50	theme	PIK3CG/AKT/BCL2	2468:2482	arg1	pathway					2484:2490	the PIK3CG/AKT/BCL2 pathway	2464:2490	the PIK3CG/AKT/BCL2 pathway	2464:2490	Our data established that APS intervenes with TNBC cell invasion, proliferation, and apoptosis via the PIK3CG/AKT/BCL2 pathway and could thus offer a promising therapeutic strategy for TNBC.
31680955	3	51	theme	composition	555:565	arg1	mechanism					531:539	the potential mechanism	517:539	the potential mechanism of the target composition acting on TNBC	517:580	In this study, the potential mechanism of the target composition acting on TNBC was explored by integrated pharmacological models and in vitro experiments.
31680955	9	52	theme	correlated	1614:1623	arg1	pathways					1635:1642	correlated signaling pathways	1614:1642	correlated signaling pathways	1614:1642	We also performed western blot analysis of key proteins to probe the mechanisms of correlated signaling pathways.
31680955	13	53	dep	in	1993:1994	arg1	vitro					1996:2000	vitro	1996:2000	vitro	1996:2000	The in vitro experimental results confirmed that APS can effectively inhibit TNBC cell activity, reduce invasion, promote apoptosis, and then counteract TNBC symptoms in a dose-dependent manner, most likely by inhibiting the PIK3CG/AKT/BCL2 pathway.
31680955	1	54	theme	natural	175:181	arg1	products					183:190	the natural products	171:190	the natural products of Astragalus membranaceus (AM)	171:222	Background: Studies have shown that the natural products of Astragalus membranaceus (AM) can effectively interfere with a variety of cancers, but their mechanism of action on breast cancer remains unclear.
31680955	2	55	theme	invasive	434:441	arg1	phenotype					443:451	its invasive phenotype	430:451	its invasive phenotype	430:451	Triple-negative breast cancer (TNBC) is associated with a severely poor prognosis due to its invasive phenotype and lack of biomarker-driven-targeted therapies.
31680955	9	56	theme	pathways	1635:1642	arg1	mechanisms					1600:1609	the mechanisms	1596:1609	the mechanisms of correlated signaling pathways	1596:1642	We also performed western blot analysis of key proteins to probe the mechanisms of correlated signaling pathways.
31680955	13	57	theme	PIK3CG/AKT/BCL2	2214:2228	arg1	pathway					2230:2236	the PIK3CG/AKT/BCL2 pathway	2210:2236	the PIK3CG/AKT/BCL2 pathway	2210:2236	The in vitro experimental results confirmed that APS can effectively inhibit TNBC cell activity, reduce invasion, promote apoptosis, and then counteract TNBC symptoms in a dose-dependent manner, most likely by inhibiting the PIK3CG/AKT/BCL2 pathway.
31680955	6	58	theme	combination	1229:1239	arg1	models					1241:1246	accurate combination models	1220:1246	accurate combination models	1220:1246	Molecular docking was used to evaluate the affinity between compounds and targets and obtain accurate combination models.
31680955	10	59	theme	compound-disease	1718:1733	arg1	"					1734:1734	"compound-disease"	1717:1734	"compound-disease" (414 nodes and 6458 edges)	1717:1761	Results: We constructed "compound-target" (339 nodes and 695 edges) and "compound-disease" (414 nodes and 6458 edges) networks using interaction data.
31680955	4	60	theme	target	815:820	arg1	components					822:831	the drug and target components	802:831	components	822:831	Materials and Methods: Based on the Gene Expression Omnibus (GEO) database and the relational database of Traditional Chinese Medicines (TCMs), the drug and target components were initially screened to construct a common network module, and multiattribute analysis was then used to characterize the network and obtain key drug-target information.
31680955	6	61	theme	Molecular	1127:1135	arg1	docking					1137:1143	Molecular docking	1127:1143	Molecular docking	1127:1143	Molecular docking was used to evaluate the affinity between compounds and targets and obtain accurate combination models.
31680955	6	62	used	used	1149:1152	arg2	docking					1137:1143	Molecular docking	1127:1143	Molecular docking	1127:1143	Molecular docking was used to evaluate the affinity between compounds and targets and obtain accurate combination models.
31680955	1	63	theme	membranaceus	206:217	arg1	products					183:190	the natural products	171:190	the natural products of Astragalus membranaceus (AM)	171:222	Background: Studies have shown that the natural products of Astragalus membranaceus (AM) can effectively interfere with a variety of cancers, but their mechanism of action on breast cancer remains unclear.
31680955	10	64	dep	"	1734:1734	arg1	nodes					1741:1745	414 nodes	1737:1745	414 nodes	1737:1745	Results: We constructed "compound-target" (339 nodes and 695 edges) and "compound-disease" (414 nodes and 6458 edges) networks using interaction data.
31680955	10	64	dep	"	1734:1734	arg1	edges					1756:1760	6458 edges	1751:1760	6458 edges	1751:1760	Results: We constructed "compound-target" (339 nodes and 695 edges) and "compound-disease" (414 nodes and 6458 edges) networks using interaction data.
31680955	15	65	theme	therapeutic	2525:2535	arg1	strategy					2537:2544	a promising therapeutic strategy	2513:2544	a promising therapeutic strategy for TNBC	2513:2553	Our data established that APS intervenes with TNBC cell invasion, proliferation, and apoptosis via the PIK3CG/AKT/BCL2 pathway and could thus offer a promising therapeutic strategy for TNBC.
31680955	14	66	theme	polypharmacology-based	2323:2344	arg1	value					2358:2362	a polypharmacology-based therapeutic value	2321:2362	a polypharmacology-based therapeutic value	2321:2362	Conclusion: This study provides a rational approach to discovering compounds with a polypharmacology-based therapeutic value.
31680955	8	67	theme	cell	1419:1422	arg1	viability					1424:1432	cell viability	1419:1432	cell viability	1419:1432	The cell counting kit-8 (CCK-8) assay, invasion assay, and flow cytometric analysis were used to assess cell viability, invasiveness, and apoptosis, respectively, after Astragalus polysaccharides (APS) intervention.
31680955	15	68	theme	TNBC	2411:2414	arg1	invasion					2421:2428	TNBC cell invasion	2411:2428	TNBC cell invasion	2411:2428	Our data established that APS intervenes with TNBC cell invasion, proliferation, and apoptosis via the PIK3CG/AKT/BCL2 pathway and could thus offer a promising therapeutic strategy for TNBC.
31680955	13	69	theme	dose-dependent	2161:2174	arg1	manner					2176:2181	a dose-dependent manner	2159:2181	a dose-dependent manner	2159:2181	The in vitro experimental results confirmed that APS can effectively inhibit TNBC cell activity, reduce invasion, promote apoptosis, and then counteract TNBC symptoms in a dose-dependent manner, most likely by inhibiting the PIK3CG/AKT/BCL2 pathway.
31680955	4	70	theme	Gene	694:697	arg1	database					724:731	the Gene Expression Omnibus (GEO) database	690:731	the Gene Expression Omnibus (GEO) database	690:731	Materials and Methods: Based on the Gene Expression Omnibus (GEO) database and the relational database of Traditional Chinese Medicines (TCMs), the drug and target components were initially screened to construct a common network module, and multiattribute analysis was then used to characterize the network and obtain key drug-target information.
31680955	5	71	used	used	1048:1051	arg2	analysis					1035:1042	network topology analysis	1018:1042	network topology analysis	1018:1042	Furthermore, network topology analysis was used to characterize the betweenness and closeness of key hubs in the network.
31680955	5	72	theme	topology	1026:1033	arg1	analysis					1035:1042	network topology analysis	1018:1042	network topology analysis	1018:1042	Furthermore, network topology analysis was used to characterize the betweenness and closeness of key hubs in the network.
31680955	3	73	theme	pharmacological	609:623	arg1	models					625:630	integrated pharmacological models	598:630	integrated pharmacological models	598:630	In this study, the potential mechanism of the target composition acting on TNBC was explored by integrated pharmacological models and in vitro experiments.
31680955	4	74	theme	common	872:877	arg1	module					887:892	a common network module	870:892	a common network module	870:892	Materials and Methods: Based on the Gene Expression Omnibus (GEO) database and the relational database of Traditional Chinese Medicines (TCMs), the drug and target components were initially screened to construct a common network module, and multiattribute analysis was then used to characterize the network and obtain key drug-target information.
31680955	4	75	theme	relational	741:750	arg1	database					752:759	the relational database	737:759	the relational database of Traditional Chinese Medicines (TCMs)	737:799	Materials and Methods: Based on the Gene Expression Omnibus (GEO) database and the relational database of Traditional Chinese Medicines (TCMs), the drug and target components were initially screened to construct a common network module, and multiattribute analysis was then used to characterize the network and obtain key drug-target information.
31680955	4	76	theme	Omnibus	710:716	arg1	database					724:731	the Gene Expression Omnibus (GEO) database	690:731	the Gene Expression Omnibus (GEO) database	690:731	Materials and Methods: Based on the Gene Expression Omnibus (GEO) database and the relational database of Traditional Chinese Medicines (TCMs), the drug and target components were initially screened to construct a common network module, and multiattribute analysis was then used to characterize the network and obtain key drug-target information.
31680955	4	77	theme	Chinese	776:782	arg1	Medicines					784:792	Traditional Chinese Medicines	764:792	Traditional Chinese Medicines (TCMs)	764:799	Materials and Methods: Based on the Gene Expression Omnibus (GEO) database and the relational database of Traditional Chinese Medicines (TCMs), the drug and target components were initially screened to construct a common network module, and multiattribute analysis was then used to characterize the network and obtain key drug-target information.
31680955	4	77	theme	Chinese	776:782	arg1	TCMs					795:798	TCMs	795:798	TCMs	795:798	Materials and Methods: Based on the Gene Expression Omnibus (GEO) database and the relational database of Traditional Chinese Medicines (TCMs), the drug and target components were initially screened to construct a common network module, and multiattribute analysis was then used to characterize the network and obtain key drug-target information.
31680955	0	78	theme	Breast	82:87	arg1	Cancer					89:94	Triple-Negative Breast Cancer	66:94	Triple-Negative Breast Cancer	66:94	The Modulatory Properties of Astragalus membranaceus Treatment on Triple-Negative Breast Cancer: An Integrated Pharmacological Method.
31680955	13	79	theme	experimental	2002:2013	arg1	results					2015:2021	The in vitro experimental results	1989:2021	The in vitro experimental results	1989:2021	The in vitro experimental results confirmed that APS can effectively inhibit TNBC cell activity, reduce invasion, promote apoptosis, and then counteract TNBC symptoms in a dose-dependent manner, most likely by inhibiting the PIK3CG/AKT/BCL2 pathway.
31680955	10	80	theme	6458	1751:1754	arg1	edges					1756:1760	6458 edges	1751:1760	6458 edges	1751:1760	Results: We constructed "compound-target" (339 nodes and 695 edges) and "compound-disease" (414 nodes and 6458 edges) networks using interaction data.
31680955	13	81	theme	TNBC	2142:2145	arg1	symptoms					2147:2154	TNBC symptoms	2142:2154	TNBC symptoms	2142:2154	The in vitro experimental results confirmed that APS can effectively inhibit TNBC cell activity, reduce invasion, promote apoptosis, and then counteract TNBC symptoms in a dose-dependent manner, most likely by inhibiting the PIK3CG/AKT/BCL2 pathway.
31680955	2	82	theme	therapies	491:499	arg1	phenotype					443:451	its invasive phenotype	430:451	its invasive phenotype	430:451	Triple-negative breast cancer (TNBC) is associated with a severely poor prognosis due to its invasive phenotype and lack of biomarker-driven-targeted therapies.
31680955	2	82	theme	therapies	491:499	arg1	lack					457:460	lack	457:460	lack of biomarker-driven-targeted therapies	457:499	Triple-negative breast cancer (TNBC) is associated with a severely poor prognosis due to its invasive phenotype and lack of biomarker-driven-targeted therapies.
31680955	11	83	theme	Topology	1796:1803	arg1	screens					1859:1865	secondary screens	1849:1865	secondary screens	1849:1865	Topology analysis and molecular docking were used as secondary screens to identify key hubs of the network.
31680955	11	83	theme	Topology	1796:1803	arg1	analysis					1805:1812	Topology analysis	1796:1812	Topology analysis	1796:1812	Topology analysis and molecular docking were used as secondary screens to identify key hubs of the network.
31680955	11	83	theme	Topology	1796:1803	arg1	docking					1828:1834	molecular docking	1818:1834	molecular docking	1818:1834	Topology analysis and molecular docking were used as secondary screens to identify key hubs of the network.
31680955	5	84	theme	hubs	1106:1109	arg1	closeness					1089:1097	closeness	1089:1097	closeness	1089:1097	Furthermore, network topology analysis was used to characterize the betweenness and closeness of key hubs in the network.
31680955	5	84	theme	hubs	1106:1109	arg1	betweenness					1073:1083	betweenness	1073:1083	betweenness	1073:1083	Furthermore, network topology analysis was used to characterize the betweenness and closeness of key hubs in the network.
31680955	0	85	theme	Integrated	100:109	arg1	Method					127:132	An Integrated Pharmacological Method	97:132	An Integrated Pharmacological Method	97:132	The Modulatory Properties of Astragalus membranaceus Treatment on Triple-Negative Breast Cancer: An Integrated Pharmacological Method.
31680955	4	86	theme	Traditional	764:774	arg1	Medicines					784:792	Traditional Chinese Medicines	764:792	Traditional Chinese Medicines (TCMs)	764:799	Materials and Methods: Based on the Gene Expression Omnibus (GEO) database and the relational database of Traditional Chinese Medicines (TCMs), the drug and target components were initially screened to construct a common network module, and multiattribute analysis was then used to characterize the network and obtain key drug-target information.
31680955	4	86	theme	Traditional	764:774	arg1	TCMs					795:798	TCMs	795:798	TCMs	795:798	Materials and Methods: Based on the Gene Expression Omnibus (GEO) database and the relational database of Traditional Chinese Medicines (TCMs), the drug and target components were initially screened to construct a common network module, and multiattribute analysis was then used to characterize the network and obtain key drug-target information.
31680955	0	87	dep	Method	127:132	arg1	Properties					15:24	The Modulatory Properties	0:24	The Modulatory Properties of Astragalus membranaceus Treatment on Triple-Negative Breast Cancer	0:94	The Modulatory Properties of Astragalus membranaceus Treatment on Triple-Negative Breast Cancer: An Integrated Pharmacological Method.
31680955	3	88	theme	target	548:553	arg1	composition					555:565	the target composition	544:565	the target composition acting on TNBC	544:580	In this study, the potential mechanism of the target composition acting on TNBC was explored by integrated pharmacological models and in vitro experiments.
31680955	8	89	theme	cell	1319:1322	arg1	kit-8					1333:1337	The cell counting kit-8	1315:1337	The cell counting kit-8 (CCK-8) assay	1315:1351	The cell counting kit-8 (CCK-8) assay, invasion assay, and flow cytometric analysis were used to assess cell viability, invasiveness, and apoptosis, respectively, after Astragalus polysaccharides (APS) intervention.
31680955	8	89	theme	cell	1319:1322	arg1	CCK-8					1340:1344	CCK-8	1340:1344	CCK-8	1340:1344	The cell counting kit-8 (CCK-8) assay, invasion assay, and flow cytometric analysis were used to assess cell viability, invasiveness, and apoptosis, respectively, after Astragalus polysaccharides (APS) intervention.
31680955	7	90	dep	in	1258:1259	arg1	vitro					1261:1265	vitro	1261:1265	vitro	1261:1265	Finally, in vitro experiments verified the key component targets.
31680955	8	91	theme	polysaccharides	1495:1509	arg1	intervention					1517:1528	Astragalus polysaccharides (APS) intervention	1484:1528	Astragalus polysaccharides (APS) intervention	1484:1528	The cell counting kit-8 (CCK-8) assay, invasion assay, and flow cytometric analysis were used to assess cell viability, invasiveness, and apoptosis, respectively, after Astragalus polysaccharides (APS) intervention.
31680955	8	92	theme	kit-8	1333:1337	arg1	assay					1347:1351	The cell counting kit-8 (CCK-8) assay	1315:1351	The cell counting kit-8 (CCK-8) assay	1315:1351	The cell counting kit-8 (CCK-8) assay, invasion assay, and flow cytometric analysis were used to assess cell viability, invasiveness, and apoptosis, respectively, after Astragalus polysaccharides (APS) intervention.
31680955	0	93	theme	Modulatory	4:13	arg1	Properties					15:24	The Modulatory Properties	0:24	The Modulatory Properties of Astragalus membranaceus Treatment on Triple-Negative Breast Cancer	0:94	The Modulatory Properties of Astragalus membranaceus Treatment on Triple-Negative Breast Cancer: An Integrated Pharmacological Method.
31680955	7	94	theme	component	1296:1304	arg1	targets					1306:1312	the key component targets	1288:1312	the key component targets	1288:1312	Finally, in vitro experiments verified the key component targets.
31680955	4	95	theme	GEO	719:721	arg1	database					724:731	the Gene Expression Omnibus (GEO) database	690:731	the Gene Expression Omnibus (GEO) database	690:731	Materials and Methods: Based on the Gene Expression Omnibus (GEO) database and the relational database of Traditional Chinese Medicines (TCMs), the drug and target components were initially screened to construct a common network module, and multiattribute analysis was then used to characterize the network and obtain key drug-target information.
31680955	5	96	from	betweenness	1073:1083	arg1	network					1118:1124	the network	1114:1124	the network	1114:1124	Furthermore, network topology analysis was used to characterize the betweenness and closeness of key hubs in the network.
31680955	0	97	theme	membranaceus	40:51	arg1	Treatment					53:61	Astragalus membranaceus Treatment	29:61	Astragalus membranaceus Treatment	29:61	The Modulatory Properties of Astragalus membranaceus Treatment on Triple-Negative Breast Cancer: An Integrated Pharmacological Method.
31680955	9	98	theme	western	1549:1555	arg1	analysis					1562:1569	western blot analysis	1549:1569	western blot analysis of key proteins	1549:1585	We also performed western blot analysis of key proteins to probe the mechanisms of correlated signaling pathways.
31680955	8	99	theme	invasion	1354:1361	arg1	assay					1363:1367	invasion assay	1354:1367	invasion assay	1354:1367	The cell counting kit-8 (CCK-8) assay, invasion assay, and flow cytometric analysis were used to assess cell viability, invasiveness, and apoptosis, respectively, after Astragalus polysaccharides (APS) intervention.
31680955	11	100	theme	secondary	1849:1857	arg1	analysis					1805:1812	Topology analysis	1796:1812	Topology analysis	1796:1812	Topology analysis and molecular docking were used as secondary screens to identify key hubs of the network.
31680955	11	100	theme	secondary	1849:1857	arg1	screens					1859:1865	secondary screens	1849:1865	secondary screens	1849:1865	Topology analysis and molecular docking were used as secondary screens to identify key hubs of the network.
31680955	11	100	theme	secondary	1849:1857	arg1	docking					1828:1834	molecular docking	1818:1834	molecular docking	1818:1834	Topology analysis and molecular docking were used as secondary screens to identify key hubs of the network.
31680955	1	101	theme	action	300:305	arg1	mechanism					287:295	their mechanism	281:295	their mechanism of action on breast cancer	281:322	Background: Studies have shown that the natural products of Astragalus membranaceus (AM) can effectively interfere with a variety of cancers, but their mechanism of action on breast cancer remains unclear.
31680955	7	102	theme	in	1258:1259	arg1	experiments					1267:1277	in vitro experiments	1258:1277	in vitro experiments	1258:1277	Finally, in vitro experiments verified the key component targets.
31680955	4	103	theme	key	976:978	arg1	information					992:1002	key drug-target information	976:1002	key drug-target information	976:1002	Materials and Methods: Based on the Gene Expression Omnibus (GEO) database and the relational database of Traditional Chinese Medicines (TCMs), the drug and target components were initially screened to construct a common network module, and multiattribute analysis was then used to characterize the network and obtain key drug-target information.
31680955	5	104	dep	betweenness	1073:1083	arg1	the					1069:1071	the	1069:1071	the	1069:1071	Furthermore, network topology analysis was used to characterize the betweenness and closeness of key hubs in the network.
31680955	12	105	dep	biomarkers	1939:1948	arg1	biomarkers					1939:1948	biomarkers	1939:1948	biomarkers PIK3CG, AKT, and BCL2	1939:1970	Finally, the key component APS and biomarkers PIK3CG, AKT, and BCL2 were identified.
31680955	12	105	dep	biomarkers	1939:1948	arg1	AKT					1958:1960	AKT	1958:1960	AKT	1958:1960	Finally, the key component APS and biomarkers PIK3CG, AKT, and BCL2 were identified.
31680955	12	105	dep	biomarkers	1939:1948	arg1	BCL2					1967:1970	BCL2	1967:1970	BCL2	1967:1970	Finally, the key component APS and biomarkers PIK3CG, AKT, and BCL2 were identified.
31680955	12	105	dep	biomarkers	1939:1948	arg1	PIK3CG					1950:1955	PIK3CG	1950:1955	PIK3CG	1950:1955	Finally, the key component APS and biomarkers PIK3CG, AKT, and BCL2 were identified.
31680955	14	106	theme	therapeutic	2346:2356	arg1	value					2358:2362	a polypharmacology-based therapeutic value	2321:2362	a polypharmacology-based therapeutic value	2321:2362	Conclusion: This study provides a rational approach to discovering compounds with a polypharmacology-based therapeutic value.
31680955	1	107	theme	breast	310:315	arg1	cancer					317:322	breast cancer	310:322	breast cancer	310:322	Background: Studies have shown that the natural products of Astragalus membranaceus (AM) can effectively interfere with a variety of cancers, but their mechanism of action on breast cancer remains unclear.
31680955	9	108	theme	key	1574:1576	arg1	proteins					1578:1585	key proteins	1574:1585	key proteins	1574:1585	We also performed western blot analysis of key proteins to probe the mechanisms of correlated signaling pathways.
31680955	8	109	theme	flow	1374:1377	arg1	analysis					1390:1397	flow cytometric analysis	1374:1397	flow cytometric analysis	1374:1397	The cell counting kit-8 (CCK-8) assay, invasion assay, and flow cytometric analysis were used to assess cell viability, invasiveness, and apoptosis, respectively, after Astragalus polysaccharides (APS) intervention.
31680955	3	110	dep	in	636:637	arg1	vitro					639:643	vitro	639:643	vitro	639:643	In this study, the potential mechanism of the target composition acting on TNBC was explored by integrated pharmacological models and in vitro experiments.
31680955	4	111	dep	Materials	658:666	arg1	used					932:935	used	932:935	was then used to characterize the network and obtain key drug-target information	923:1002	Materials and Methods: Based on the Gene Expression Omnibus (GEO) database and the relational database of Traditional Chinese Medicines (TCMs), the drug and target components were initially screened to construct a common network module, and multiattribute analysis was then used to characterize the network and obtain key drug-target information.
31680955	4	111	dep	Materials	658:666	arg1	screened					848:855	screened	848:855	were initially screened to construct a common network module	833:892	Materials and Methods: Based on the Gene Expression Omnibus (GEO) database and the relational database of Traditional Chinese Medicines (TCMs), the drug and target components were initially screened to construct a common network module, and multiattribute analysis was then used to characterize the network and obtain key drug-target information.
31680955	10	112	dep	"	1685:1685	arg1	nodes					1692:1696	339 nodes	1688:1696	339 nodes	1688:1696	Results: We constructed "compound-target" (339 nodes and 695 edges) and "compound-disease" (414 nodes and 6458 edges) networks using interaction data.
31680955	10	112	dep	"	1685:1685	arg1	edges					1706:1710	695 edges	1702:1710	695 edges	1702:1710	Results: We constructed "compound-target" (339 nodes and 695 edges) and "compound-disease" (414 nodes and 6458 edges) networks using interaction data.
31680955	10	112	dep	"	1685:1685	arg1	networks					1763:1770	networks	1763:1770	networks	1763:1770	Results: We constructed "compound-target" (339 nodes and 695 edges) and "compound-disease" (414 nodes and 6458 edges) networks using interaction data.
31680955	4	113	theme	Medicines	784:792	arg1	database					724:731	the Gene Expression Omnibus (GEO) database	690:731	the Gene Expression Omnibus (GEO) database	690:731	Materials and Methods: Based on the Gene Expression Omnibus (GEO) database and the relational database of Traditional Chinese Medicines (TCMs), the drug and target components were initially screened to construct a common network module, and multiattribute analysis was then used to characterize the network and obtain key drug-target information.
31680955	4	113	theme	Medicines	784:792	arg1	database					752:759	the relational database	737:759	the relational database of Traditional Chinese Medicines (TCMs)	737:799	Materials and Methods: Based on the Gene Expression Omnibus (GEO) database and the relational database of Traditional Chinese Medicines (TCMs), the drug and target components were initially screened to construct a common network module, and multiattribute analysis was then used to characterize the network and obtain key drug-target information.
31680955	5	114	from	closeness	1089:1097	arg1	network					1118:1124	the network	1114:1124	the network	1114:1124	Furthermore, network topology analysis was used to characterize the betweenness and closeness of key hubs in the network.
31746805	4	0	theme	mg	810:811	arg1	concentration					789:801	a higher natural coagulant (NC) concentration	757:801	a higher natural coagulant (NC) concentration (15.00 mg L-1)	757:816	We were able to analyze the synergistic effects between the FeCl3.6H2O and mucilage concentrations: the process of coagulation/flocculation (CF) for chemical oxygen demand (COD) removal was more efficient at a low chemical coagulant concentration (8.00 mg L-1) and a higher natural coagulant (NC) concentration (15.00 mg L-1).
31746805	4	0	theme	mg	810:811	arg1	L-1					813:815	15.00 mg L-1	804:815	15.00 mg L-1	804:815	We were able to analyze the synergistic effects between the FeCl3.6H2O and mucilage concentrations: the process of coagulation/flocculation (CF) for chemical oxygen demand (COD) removal was more efficient at a low chemical coagulant concentration (8.00 mg L-1) and a higher natural coagulant (NC) concentration (15.00 mg L-1).
31746805	0	1	theme	Dillenia	86:93	arg1	indica					95:100	Dillenia indica	86:100	Dillenia indica	86:100	Coagulation/flocculation of textile effluent using a natural coagulant extracted from Dillenia indica.
31746805	6	2	dep	Fourier	1055:1061	arg1	transform					1063:1071	transform	1063:1071	transform infrared spectroscopy (FTIR) and solid-state cross-polarization magic angle spinning carbon-13 nuclear magnetic resonance (CP/MAS 13C NMR)	1063:1210	The characterization of the mucilage of Dillenia indica was done using Fourier transform infrared spectroscopy (FTIR) and solid-state cross-polarization magic angle spinning carbon-13 nuclear magnetic resonance (CP/MAS 13C NMR), and the signals obtained indicated the presence of polysaccharides, which are responsible for enhancing the CF process.
31746805	4	3	theme	FeCl3.6H2O	552:561	arg1	concentrations					576:589	the FeCl3.6H2O and mucilage concentrations	548:589	the FeCl3.6H2O and mucilage concentrations	548:589	We were able to analyze the synergistic effects between the FeCl3.6H2O and mucilage concentrations: the process of coagulation/flocculation (CF) for chemical oxygen demand (COD) removal was more efficient at a low chemical coagulant concentration (8.00 mg L-1) and a higher natural coagulant (NC) concentration (15.00 mg L-1).
31746805	5	4	theme	COD	909:911	arg1	removal					913:919	67.66%% COD removal	901:919	67.66%% COD removal	901:919	This demonstrated the potential of this mucilage to treat textile effluents, with 67.66%% COD removal, 96.86% turbidity removal and 91.12% apparent color reduction.
31746805	6	5	theme	13C	1203:1205	arg1	resonance					1185:1193	carbon-13 nuclear magnetic resonance	1158:1193	carbon-13 nuclear magnetic resonance (CP/MAS 13C NMR)	1158:1210	The characterization of the mucilage of Dillenia indica was done using Fourier transform infrared spectroscopy (FTIR) and solid-state cross-polarization magic angle spinning carbon-13 nuclear magnetic resonance (CP/MAS 13C NMR), and the signals obtained indicated the presence of polysaccharides, which are responsible for enhancing the CF process.
31746805	6	5	theme	13C	1203:1205	arg1	NMR					1207:1209	CP/MAS 13C NMR	1196:1209	CP/MAS 13C NMR	1196:1209	The characterization of the mucilage of Dillenia indica was done using Fourier transform infrared spectroscopy (FTIR) and solid-state cross-polarization magic angle spinning carbon-13 nuclear magnetic resonance (CP/MAS 13C NMR), and the signals obtained indicated the presence of polysaccharides, which are responsible for enhancing the CF process.
31746805	2	6	theme	mucilage	278:285	arg1	extraction					287:296	The mucilage extraction	274:296	The mucilage extraction	274:296	The mucilage extraction was carried out in water at room temperature.
31746805	4	7	theme	chemical	641:648	arg1	removal					670:676	chemical oxygen demand (COD) removal	641:676	chemical oxygen demand (COD) removal	641:676	We were able to analyze the synergistic effects between the FeCl3.6H2O and mucilage concentrations: the process of coagulation/flocculation (CF) for chemical oxygen demand (COD) removal was more efficient at a low chemical coagulant concentration (8.00 mg L-1) and a higher natural coagulant (NC) concentration (15.00 mg L-1).
31746805	6	8	theme	carbon-13	1158:1166	arg1	resonance					1185:1193	carbon-13 nuclear magnetic resonance	1158:1193	carbon-13 nuclear magnetic resonance (CP/MAS 13C NMR)	1158:1210	The characterization of the mucilage of Dillenia indica was done using Fourier transform infrared spectroscopy (FTIR) and solid-state cross-polarization magic angle spinning carbon-13 nuclear magnetic resonance (CP/MAS 13C NMR), and the signals obtained indicated the presence of polysaccharides, which are responsible for enhancing the CF process.
31746805	6	8	theme	carbon-13	1158:1166	arg1	NMR					1207:1209	CP/MAS 13C NMR	1196:1209	CP/MAS 13C NMR	1196:1209	The characterization of the mucilage of Dillenia indica was done using Fourier transform infrared spectroscopy (FTIR) and solid-state cross-polarization magic angle spinning carbon-13 nuclear magnetic resonance (CP/MAS 13C NMR), and the signals obtained indicated the presence of polysaccharides, which are responsible for enhancing the CF process.
31746805	6	9	theme	CP/MAS	1196:1201	arg1	resonance					1185:1193	carbon-13 nuclear magnetic resonance	1158:1193	carbon-13 nuclear magnetic resonance (CP/MAS 13C NMR)	1158:1210	The characterization of the mucilage of Dillenia indica was done using Fourier transform infrared spectroscopy (FTIR) and solid-state cross-polarization magic angle spinning carbon-13 nuclear magnetic resonance (CP/MAS 13C NMR), and the signals obtained indicated the presence of polysaccharides, which are responsible for enhancing the CF process.
31746805	6	9	theme	CP/MAS	1196:1201	arg1	NMR					1207:1209	CP/MAS 13C NMR	1196:1209	CP/MAS 13C NMR	1196:1209	The characterization of the mucilage of Dillenia indica was done using Fourier transform infrared spectroscopy (FTIR) and solid-state cross-polarization magic angle spinning carbon-13 nuclear magnetic resonance (CP/MAS 13C NMR), and the signals obtained indicated the presence of polysaccharides, which are responsible for enhancing the CF process.
31746805	6	10	theme	solid-state	1106:1116	arg1	angle					1143:1147	solid-state cross-polarization magic angle	1106:1147	solid-state cross-polarization magic angle spinning carbon-13 nuclear magnetic resonance (CP/MAS 13C NMR)	1106:1210	The characterization of the mucilage of Dillenia indica was done using Fourier transform infrared spectroscopy (FTIR) and solid-state cross-polarization magic angle spinning carbon-13 nuclear magnetic resonance (CP/MAS 13C NMR), and the signals obtained indicated the presence of polysaccharides, which are responsible for enhancing the CF process.
31746805	1	11	theme	textile	256:262	arg1	effluent					264:271	textile effluent	256:271	textile effluent	256:271	The aim of this study was to assess the efficiency of mucilage extracted from the fruit of Dillenia indica for enhancing coagulation in the treatment of textile effluent.
31746805	6	12	theme	polysaccharides	1264:1278	arg1	presence					1252:1259	the presence	1248:1259	the presence of polysaccharides, which are responsible for enhancing the CF process	1248:1330	The characterization of the mucilage of Dillenia indica was done using Fourier transform infrared spectroscopy (FTIR) and solid-state cross-polarization magic angle spinning carbon-13 nuclear magnetic resonance (CP/MAS 13C NMR), and the signals obtained indicated the presence of polysaccharides, which are responsible for enhancing the CF process.
31746805	4	13	from	concentration	789:801	arg1	process					596:602	the process	592:602	the process of coagulation/flocculation (CF) for chemical oxygen demand (COD) removal	592:676	We were able to analyze the synergistic effects between the FeCl3.6H2O and mucilage concentrations: the process of coagulation/flocculation (CF) for chemical oxygen demand (COD) removal was more efficient at a low chemical coagulant concentration (8.00 mg L-1) and a higher natural coagulant (NC) concentration (15.00 mg L-1).
31746805	4	13	from	concentration	789:801	arg1	efficient					687:695	efficient	687:695	efficient	687:695	We were able to analyze the synergistic effects between the FeCl3.6H2O and mucilage concentrations: the process of coagulation/flocculation (CF) for chemical oxygen demand (COD) removal was more efficient at a low chemical coagulant concentration (8.00 mg L-1) and a higher natural coagulant (NC) concentration (15.00 mg L-1).
31746805	4	14	theme	coagulation/flocculation	607:630	arg1	efficient					687:695	efficient	687:695	efficient	687:695	We were able to analyze the synergistic effects between the FeCl3.6H2O and mucilage concentrations: the process of coagulation/flocculation (CF) for chemical oxygen demand (COD) removal was more efficient at a low chemical coagulant concentration (8.00 mg L-1) and a higher natural coagulant (NC) concentration (15.00 mg L-1).
31746805	4	14	theme	coagulation/flocculation	607:630	arg1	process					596:602	the process	592:602	the process of coagulation/flocculation (CF) for chemical oxygen demand (COD) removal	592:676	We were able to analyze the synergistic effects between the FeCl3.6H2O and mucilage concentrations: the process of coagulation/flocculation (CF) for chemical oxygen demand (COD) removal was more efficient at a low chemical coagulant concentration (8.00 mg L-1) and a higher natural coagulant (NC) concentration (15.00 mg L-1).
31746805	4	15	theme	mg	745:746	arg1	L-1					748:750	8.00 mg L-1	740:750	8.00 mg L-1	740:750	We were able to analyze the synergistic effects between the FeCl3.6H2O and mucilage concentrations: the process of coagulation/flocculation (CF) for chemical oxygen demand (COD) removal was more efficient at a low chemical coagulant concentration (8.00 mg L-1) and a higher natural coagulant (NC) concentration (15.00 mg L-1).
31746805	4	15	theme	mg	745:746	arg1	concentration					725:737	a low chemical coagulant concentration	700:737	a low chemical coagulant concentration (8.00 mg L-1)	700:751	We were able to analyze the synergistic effects between the FeCl3.6H2O and mucilage concentrations: the process of coagulation/flocculation (CF) for chemical oxygen demand (COD) removal was more efficient at a low chemical coagulant concentration (8.00 mg L-1) and a higher natural coagulant (NC) concentration (15.00 mg L-1).
31746805	6	16	dep	transform	1063:1071	arg1	infrared					1073:1080	infrared	1073:1080	transform infrared spectroscopy (FTIR) and solid-state cross-polarization magic angle spinning carbon-13 nuclear magnetic resonance (CP/MAS 13C NMR)	1063:1210	The characterization of the mucilage of Dillenia indica was done using Fourier transform infrared spectroscopy (FTIR) and solid-state cross-polarization magic angle spinning carbon-13 nuclear magnetic resonance (CP/MAS 13C NMR), and the signals obtained indicated the presence of polysaccharides, which are responsible for enhancing the CF process.
31746805	1	17	theme	mucilage	157:164	arg1	efficiency					143:152	the efficiency	139:152	the efficiency of mucilage extracted from the fruit of Dillenia indica for enhancing coagulation in the treatment of textile effluent	139:271	The aim of this study was to assess the efficiency of mucilage extracted from the fruit of Dillenia indica for enhancing coagulation in the treatment of textile effluent.
31746805	4	18	theme	mucilage	567:574	arg1	concentrations					576:589	the FeCl3.6H2O and mucilage concentrations	548:589	the FeCl3.6H2O and mucilage concentrations	548:589	We were able to analyze the synergistic effects between the FeCl3.6H2O and mucilage concentrations: the process of coagulation/flocculation (CF) for chemical oxygen demand (COD) removal was more efficient at a low chemical coagulant concentration (8.00 mg L-1) and a higher natural coagulant (NC) concentration (15.00 mg L-1).
31746805	4	19	theme	NC	785:786	arg1	concentration					789:801	a higher natural coagulant (NC) concentration	757:801	a higher natural coagulant (NC) concentration (15.00 mg L-1)	757:816	We were able to analyze the synergistic effects between the FeCl3.6H2O and mucilage concentrations: the process of coagulation/flocculation (CF) for chemical oxygen demand (COD) removal was more efficient at a low chemical coagulant concentration (8.00 mg L-1) and a higher natural coagulant (NC) concentration (15.00 mg L-1).
31746805	4	19	theme	NC	785:786	arg1	L-1					813:815	15.00 mg L-1	804:815	15.00 mg L-1	804:815	We were able to analyze the synergistic effects between the FeCl3.6H2O and mucilage concentrations: the process of coagulation/flocculation (CF) for chemical oxygen demand (COD) removal was more efficient at a low chemical coagulant concentration (8.00 mg L-1) and a higher natural coagulant (NC) concentration (15.00 mg L-1).
31746805	5	20	theme	%	906:906	arg1	removal					913:919	67.66%% COD removal	901:919	67.66%% COD removal	901:919	This demonstrated the potential of this mucilage to treat textile effluents, with 67.66%% COD removal, 96.86% turbidity removal and 91.12% apparent color reduction.
31746805	6	21	theme	magnetic	1176:1183	arg1	resonance					1185:1193	carbon-13 nuclear magnetic resonance	1158:1193	carbon-13 nuclear magnetic resonance (CP/MAS 13C NMR)	1158:1210	The characterization of the mucilage of Dillenia indica was done using Fourier transform infrared spectroscopy (FTIR) and solid-state cross-polarization magic angle spinning carbon-13 nuclear magnetic resonance (CP/MAS 13C NMR), and the signals obtained indicated the presence of polysaccharides, which are responsible for enhancing the CF process.
31746805	6	21	theme	magnetic	1176:1183	arg1	NMR					1207:1209	CP/MAS 13C NMR	1196:1209	CP/MAS 13C NMR	1196:1209	The characterization of the mucilage of Dillenia indica was done using Fourier transform infrared spectroscopy (FTIR) and solid-state cross-polarization magic angle spinning carbon-13 nuclear magnetic resonance (CP/MAS 13C NMR), and the signals obtained indicated the presence of polysaccharides, which are responsible for enhancing the CF process.
31746805	4	22	theme	low	702:704	arg1	L-1					748:750	8.00 mg L-1	740:750	8.00 mg L-1	740:750	We were able to analyze the synergistic effects between the FeCl3.6H2O and mucilage concentrations: the process of coagulation/flocculation (CF) for chemical oxygen demand (COD) removal was more efficient at a low chemical coagulant concentration (8.00 mg L-1) and a higher natural coagulant (NC) concentration (15.00 mg L-1).
31746805	4	22	theme	low	702:704	arg1	concentration					725:737	a low chemical coagulant concentration	700:737	a low chemical coagulant concentration (8.00 mg L-1)	700:751	We were able to analyze the synergistic effects between the FeCl3.6H2O and mucilage concentrations: the process of coagulation/flocculation (CF) for chemical oxygen demand (COD) removal was more efficient at a low chemical coagulant concentration (8.00 mg L-1) and a higher natural coagulant (NC) concentration (15.00 mg L-1).
31746805	4	23	theme	demand	657:662	arg1	removal					670:676	chemical oxygen demand (COD) removal	641:676	chemical oxygen demand (COD) removal	641:676	We were able to analyze the synergistic effects between the FeCl3.6H2O and mucilage concentrations: the process of coagulation/flocculation (CF) for chemical oxygen demand (COD) removal was more efficient at a low chemical coagulant concentration (8.00 mg L-1) and a higher natural coagulant (NC) concentration (15.00 mg L-1).
31746805	3	24	theme	coagulant	369:377	arg1	FeCl3.6H2O					379:388	coagulant FeCl3.6H2O	369:388	coagulant FeCl3.6H2O	369:388	The pH, concentration of coagulant FeCl3.6H2O, and concentration of mucilage solution were optimized with star-type central composite design (CCD).
31746805	1	25	theme	effluent	264:271	arg1	treatment					243:251	the treatment	239:251	the treatment of textile effluent	239:271	The aim of this study was to assess the efficiency of mucilage extracted from the fruit of Dillenia indica for enhancing coagulation in the treatment of textile effluent.
31746805	6	26	theme	nuclear	1168:1174	arg1	resonance					1185:1193	carbon-13 nuclear magnetic resonance	1158:1193	carbon-13 nuclear magnetic resonance (CP/MAS 13C NMR)	1158:1210	The characterization of the mucilage of Dillenia indica was done using Fourier transform infrared spectroscopy (FTIR) and solid-state cross-polarization magic angle spinning carbon-13 nuclear magnetic resonance (CP/MAS 13C NMR), and the signals obtained indicated the presence of polysaccharides, which are responsible for enhancing the CF process.
31746805	6	26	theme	nuclear	1168:1174	arg1	NMR					1207:1209	CP/MAS 13C NMR	1196:1209	CP/MAS 13C NMR	1196:1209	The characterization of the mucilage of Dillenia indica was done using Fourier transform infrared spectroscopy (FTIR) and solid-state cross-polarization magic angle spinning carbon-13 nuclear magnetic resonance (CP/MAS 13C NMR), and the signals obtained indicated the presence of polysaccharides, which are responsible for enhancing the CF process.
31746805	3	27	theme	mucilage	412:419	arg1	solution					421:428	mucilage solution	412:428	mucilage solution	412:428	The pH, concentration of coagulant FeCl3.6H2O, and concentration of mucilage solution were optimized with star-type central composite design (CCD).
31746805	0	28	theme	effluent	36:43	arg1	Coagulation/flocculation					0:23	Coagulation/flocculation	0:23	Coagulation/flocculation of textile effluent	0:43	Coagulation/flocculation of textile effluent using a natural coagulant extracted from Dillenia indica.
31746805	4	29	theme	synergistic	520:530	arg1	effects					532:538	the synergistic effects	516:538	the synergistic effects between the FeCl3.6H2O and mucilage concentrations	516:589	We were able to analyze the synergistic effects between the FeCl3.6H2O and mucilage concentrations: the process of coagulation/flocculation (CF) for chemical oxygen demand (COD) removal was more efficient at a low chemical coagulant concentration (8.00 mg L-1) and a higher natural coagulant (NC) concentration (15.00 mg L-1).
31746805	4	30	from	concentration	725:737	arg1	process					596:602	the process	592:602	the process of coagulation/flocculation (CF) for chemical oxygen demand (COD) removal	592:676	We were able to analyze the synergistic effects between the FeCl3.6H2O and mucilage concentrations: the process of coagulation/flocculation (CF) for chemical oxygen demand (COD) removal was more efficient at a low chemical coagulant concentration (8.00 mg L-1) and a higher natural coagulant (NC) concentration (15.00 mg L-1).
31746805	4	30	from	concentration	725:737	arg1	efficient					687:695	efficient	687:695	efficient	687:695	We were able to analyze the synergistic effects between the FeCl3.6H2O and mucilage concentrations: the process of coagulation/flocculation (CF) for chemical oxygen demand (COD) removal was more efficient at a low chemical coagulant concentration (8.00 mg L-1) and a higher natural coagulant (NC) concentration (15.00 mg L-1).
31746805	4	31	theme	coagulant	715:723	arg1	L-1					748:750	8.00 mg L-1	740:750	8.00 mg L-1	740:750	We were able to analyze the synergistic effects between the FeCl3.6H2O and mucilage concentrations: the process of coagulation/flocculation (CF) for chemical oxygen demand (COD) removal was more efficient at a low chemical coagulant concentration (8.00 mg L-1) and a higher natural coagulant (NC) concentration (15.00 mg L-1).
31746805	4	31	theme	coagulant	715:723	arg1	concentration					725:737	a low chemical coagulant concentration	700:737	a low chemical coagulant concentration (8.00 mg L-1)	700:751	We were able to analyze the synergistic effects between the FeCl3.6H2O and mucilage concentrations: the process of coagulation/flocculation (CF) for chemical oxygen demand (COD) removal was more efficient at a low chemical coagulant concentration (8.00 mg L-1) and a higher natural coagulant (NC) concentration (15.00 mg L-1).
31746805	5	32	theme	96.86	922:926	arg1	%					927:927	%	927:927	%	927:927	This demonstrated the potential of this mucilage to treat textile effluents, with 67.66%% COD removal, 96.86% turbidity removal and 91.12% apparent color reduction.
31746805	3	33	theme	star-type	450:458	arg1	CCD					486:488	CCD	486:488	CCD	486:488	The pH, concentration of coagulant FeCl3.6H2O, and concentration of mucilage solution were optimized with star-type central composite design (CCD).
31746805	3	33	theme	star-type	450:458	arg1	design					478:483	star-type central composite design	450:483	star-type central composite design (CCD)	450:489	The pH, concentration of coagulant FeCl3.6H2O, and concentration of mucilage solution were optimized with star-type central composite design (CCD).
31746805	0	34	theme	textile	28:34	arg1	effluent					36:43	textile effluent	28:43	textile effluent	28:43	Coagulation/flocculation of textile effluent using a natural coagulant extracted from Dillenia indica.
31746805	5	35	theme	67.66	901:905	arg1	%					906:906	%	906:906	%	906:906	This demonstrated the potential of this mucilage to treat textile effluents, with 67.66%% COD removal, 96.86% turbidity removal and 91.12% apparent color reduction.
31746805	5	36	theme	color	967:971	arg1	reduction					973:981	91.12% apparent color reduction	951:981	91.12% apparent color reduction	951:981	This demonstrated the potential of this mucilage to treat textile effluents, with 67.66%% COD removal, 96.86% turbidity removal and 91.12% apparent color reduction.
31746805	4	37	theme	chemical	706:713	arg1	L-1					748:750	8.00 mg L-1	740:750	8.00 mg L-1	740:750	We were able to analyze the synergistic effects between the FeCl3.6H2O and mucilage concentrations: the process of coagulation/flocculation (CF) for chemical oxygen demand (COD) removal was more efficient at a low chemical coagulant concentration (8.00 mg L-1) and a higher natural coagulant (NC) concentration (15.00 mg L-1).
31746805	4	37	theme	chemical	706:713	arg1	concentration					725:737	a low chemical coagulant concentration	700:737	a low chemical coagulant concentration (8.00 mg L-1)	700:751	We were able to analyze the synergistic effects between the FeCl3.6H2O and mucilage concentrations: the process of coagulation/flocculation (CF) for chemical oxygen demand (COD) removal was more efficient at a low chemical coagulant concentration (8.00 mg L-1) and a higher natural coagulant (NC) concentration (15.00 mg L-1).
31746805	3	38	theme	central	460:466	arg1	CCD					486:488	CCD	486:488	CCD	486:488	The pH, concentration of coagulant FeCl3.6H2O, and concentration of mucilage solution were optimized with star-type central composite design (CCD).
31746805	3	38	theme	central	460:466	arg1	design					478:483	star-type central composite design	450:483	star-type central composite design (CCD)	450:489	The pH, concentration of coagulant FeCl3.6H2O, and concentration of mucilage solution were optimized with star-type central composite design (CCD).
31746805	6	39	theme	indica	1033:1038	arg1	mucilage					1012:1019	the mucilage	1008:1019	the mucilage of Dillenia indica	1008:1038	The characterization of the mucilage of Dillenia indica was done using Fourier transform infrared spectroscopy (FTIR) and solid-state cross-polarization magic angle spinning carbon-13 nuclear magnetic resonance (CP/MAS 13C NMR), and the signals obtained indicated the presence of polysaccharides, which are responsible for enhancing the CF process.
31746805	4	40	theme	natural	766:772	arg1	concentration					789:801	a higher natural coagulant (NC) concentration	757:801	a higher natural coagulant (NC) concentration (15.00 mg L-1)	757:816	We were able to analyze the synergistic effects between the FeCl3.6H2O and mucilage concentrations: the process of coagulation/flocculation (CF) for chemical oxygen demand (COD) removal was more efficient at a low chemical coagulant concentration (8.00 mg L-1) and a higher natural coagulant (NC) concentration (15.00 mg L-1).
31746805	4	40	theme	natural	766:772	arg1	L-1					813:815	15.00 mg L-1	804:815	15.00 mg L-1	804:815	We were able to analyze the synergistic effects between the FeCl3.6H2O and mucilage concentrations: the process of coagulation/flocculation (CF) for chemical oxygen demand (COD) removal was more efficient at a low chemical coagulant concentration (8.00 mg L-1) and a higher natural coagulant (NC) concentration (15.00 mg L-1).
31746805	4	41	theme	oxygen	650:655	arg1	removal					670:676	chemical oxygen demand (COD) removal	641:676	chemical oxygen demand (COD) removal	641:676	We were able to analyze the synergistic effects between the FeCl3.6H2O and mucilage concentrations: the process of coagulation/flocculation (CF) for chemical oxygen demand (COD) removal was more efficient at a low chemical coagulant concentration (8.00 mg L-1) and a higher natural coagulant (NC) concentration (15.00 mg L-1).
31746805	1	42	theme	Dillenia	194:201	arg1	indica					203:208	Dillenia indica	194:208	Dillenia indica for enhancing coagulation in the treatment of textile effluent	194:271	The aim of this study was to assess the efficiency of mucilage extracted from the fruit of Dillenia indica for enhancing coagulation in the treatment of textile effluent.
31746805	3	43	theme	solution	421:428	arg1	concentration					395:407	concentration	395:407	concentration of mucilage solution	395:428	The pH, concentration of coagulant FeCl3.6H2O, and concentration of mucilage solution were optimized with star-type central composite design (CCD).
31746805	3	43	theme	solution	421:428	arg1	concentration					352:364	concentration	352:364	concentration of coagulant FeCl3.6H2O	352:388	The pH, concentration of coagulant FeCl3.6H2O, and concentration of mucilage solution were optimized with star-type central composite design (CCD).
31746805	3	43	theme	solution	421:428	arg1	pH					348:349	The pH	344:349	The pH	344:349	The pH, concentration of coagulant FeCl3.6H2O, and concentration of mucilage solution were optimized with star-type central composite design (CCD).
31746805	4	44	theme	higher	759:764	arg1	concentration					789:801	a higher natural coagulant (NC) concentration	757:801	a higher natural coagulant (NC) concentration (15.00 mg L-1)	757:816	We were able to analyze the synergistic effects between the FeCl3.6H2O and mucilage concentrations: the process of coagulation/flocculation (CF) for chemical oxygen demand (COD) removal was more efficient at a low chemical coagulant concentration (8.00 mg L-1) and a higher natural coagulant (NC) concentration (15.00 mg L-1).
31746805	4	44	theme	higher	759:764	arg1	L-1					813:815	15.00 mg L-1	804:815	15.00 mg L-1	804:815	We were able to analyze the synergistic effects between the FeCl3.6H2O and mucilage concentrations: the process of coagulation/flocculation (CF) for chemical oxygen demand (COD) removal was more efficient at a low chemical coagulant concentration (8.00 mg L-1) and a higher natural coagulant (NC) concentration (15.00 mg L-1).
31746805	3	45	theme	FeCl3.6H2O	379:388	arg1	concentration					395:407	concentration	395:407	concentration of mucilage solution	395:428	The pH, concentration of coagulant FeCl3.6H2O, and concentration of mucilage solution were optimized with star-type central composite design (CCD).
31746805	3	45	theme	FeCl3.6H2O	379:388	arg1	concentration					352:364	concentration	352:364	concentration of coagulant FeCl3.6H2O	352:388	The pH, concentration of coagulant FeCl3.6H2O, and concentration of mucilage solution were optimized with star-type central composite design (CCD).
31746805	3	45	theme	FeCl3.6H2O	379:388	arg1	pH					348:349	The pH	344:349	The pH	344:349	The pH, concentration of coagulant FeCl3.6H2O, and concentration of mucilage solution were optimized with star-type central composite design (CCD).
31746805	1	46	from	coagulation	224:234	arg1	treatment					243:251	the treatment	239:251	the treatment of textile effluent	239:271	The aim of this study was to assess the efficiency of mucilage extracted from the fruit of Dillenia indica for enhancing coagulation in the treatment of textile effluent.
31746805	5	47	theme	turbidity	929:937	arg1	removal					939:945	96.86% turbidity removal	922:945	96.86% turbidity removal	922:945	This demonstrated the potential of this mucilage to treat textile effluents, with 67.66%% COD removal, 96.86% turbidity removal and 91.12% apparent color reduction.
31746805	6	48	theme	mucilage	1012:1019	arg1	characterization					988:1003	The characterization	984:1003	The characterization of the mucilage of Dillenia indica	984:1038	The characterization of the mucilage of Dillenia indica was done using Fourier transform infrared spectroscopy (FTIR) and solid-state cross-polarization magic angle spinning carbon-13 nuclear magnetic resonance (CP/MAS 13C NMR), and the signals obtained indicated the presence of polysaccharides, which are responsible for enhancing the CF process.
31746805	2	49	theme	room	326:329	arg1	temperature					331:341	room temperature	326:341	room temperature	326:341	The mucilage extraction was carried out in water at room temperature.
31746805	0	50	theme	natural	53:59	arg1	coagulant					61:69	a natural coagulant	51:69	a natural coagulant extracted from Dillenia indica	51:100	Coagulation/flocculation of textile effluent using a natural coagulant extracted from Dillenia indica.
31746805	4	51	theme	coagulant	774:782	arg1	concentration					789:801	a higher natural coagulant (NC) concentration	757:801	a higher natural coagulant (NC) concentration (15.00 mg L-1)	757:816	We were able to analyze the synergistic effects between the FeCl3.6H2O and mucilage concentrations: the process of coagulation/flocculation (CF) for chemical oxygen demand (COD) removal was more efficient at a low chemical coagulant concentration (8.00 mg L-1) and a higher natural coagulant (NC) concentration (15.00 mg L-1).
31746805	4	51	theme	coagulant	774:782	arg1	L-1					813:815	15.00 mg L-1	804:815	15.00 mg L-1	804:815	We were able to analyze the synergistic effects between the FeCl3.6H2O and mucilage concentrations: the process of coagulation/flocculation (CF) for chemical oxygen demand (COD) removal was more efficient at a low chemical coagulant concentration (8.00 mg L-1) and a higher natural coagulant (NC) concentration (15.00 mg L-1).
31746805	1	52	theme	study	119:123	arg1	aim					107:109	The aim	103:109	The aim of this study	103:123	The aim of this study was to assess the efficiency of mucilage extracted from the fruit of Dillenia indica for enhancing coagulation in the treatment of textile effluent.
31746805	6	53	theme	magic	1137:1141	arg1	angle					1143:1147	solid-state cross-polarization magic angle	1106:1147	solid-state cross-polarization magic angle spinning carbon-13 nuclear magnetic resonance (CP/MAS 13C NMR)	1106:1210	The characterization of the mucilage of Dillenia indica was done using Fourier transform infrared spectroscopy (FTIR) and solid-state cross-polarization magic angle spinning carbon-13 nuclear magnetic resonance (CP/MAS 13C NMR), and the signals obtained indicated the presence of polysaccharides, which are responsible for enhancing the CF process.
31746805	5	54	theme	91.12	951:955	arg1	%					956:956	%	956:956	%	956:956	This demonstrated the potential of this mucilage to treat textile effluents, with 67.66%% COD removal, 96.86% turbidity removal and 91.12% apparent color reduction.
31746805	5	55	theme	%	907:907	arg1	removal					913:919	67.66%% COD removal	901:919	67.66%% COD removal	901:919	This demonstrated the potential of this mucilage to treat textile effluents, with 67.66%% COD removal, 96.86% turbidity removal and 91.12% apparent color reduction.
31746805	5	56	theme	%	927:927	arg1	removal					939:945	96.86% turbidity removal	922:945	96.86% turbidity removal	922:945	This demonstrated the potential of this mucilage to treat textile effluents, with 67.66%% COD removal, 96.86% turbidity removal and 91.12% apparent color reduction.
31746805	3	57	theme	composite	468:476	arg1	CCD					486:488	CCD	486:488	CCD	486:488	The pH, concentration of coagulant FeCl3.6H2O, and concentration of mucilage solution were optimized with star-type central composite design (CCD).
31746805	3	57	theme	composite	468:476	arg1	design					478:483	star-type central composite design	450:483	star-type central composite design (CCD)	450:489	The pH, concentration of coagulant FeCl3.6H2O, and concentration of mucilage solution were optimized with star-type central composite design (CCD).
31746805	5	58	theme	textile	877:883	arg1	effluents					885:893	textile effluents	877:893	textile effluents	877:893	This demonstrated the potential of this mucilage to treat textile effluents, with 67.66%% COD removal, 96.86% turbidity removal and 91.12% apparent color reduction.
31746805	5	59	theme	%	956:956	arg1	reduction					973:981	91.12% apparent color reduction	951:981	91.12% apparent color reduction	951:981	This demonstrated the potential of this mucilage to treat textile effluents, with 67.66%% COD removal, 96.86% turbidity removal and 91.12% apparent color reduction.
31746805	1	60	dep	indica	203:208	arg1	the					181:183	the	181:183	the	181:183	The aim of this study was to assess the efficiency of mucilage extracted from the fruit of Dillenia indica for enhancing coagulation in the treatment of textile effluent.
31746805	1	60	dep	indica	203:208	arg1	fruit					185:189	fruit	185:189	fruit	185:189	The aim of this study was to assess the efficiency of mucilage extracted from the fruit of Dillenia indica for enhancing coagulation in the treatment of textile effluent.
31746805	5	61	theme	mucilage	859:866	arg1	potential					841:849	the potential	837:849	the potential of this mucilage to treat textile effluents	837:893	This demonstrated the potential of this mucilage to treat textile effluents, with 67.66%% COD removal, 96.86% turbidity removal and 91.12% apparent color reduction.
31746805	6	62	theme	Dillenia	1024:1031	arg1	indica					1033:1038	Dillenia indica	1024:1038	Dillenia indica	1024:1038	The characterization of the mucilage of Dillenia indica was done using Fourier transform infrared spectroscopy (FTIR) and solid-state cross-polarization magic angle spinning carbon-13 nuclear magnetic resonance (CP/MAS 13C NMR), and the signals obtained indicated the presence of polysaccharides, which are responsible for enhancing the CF process.
31746805	5	63	theme	apparent	958:965	arg1	reduction					973:981	91.12% apparent color reduction	951:981	91.12% apparent color reduction	951:981	This demonstrated the potential of this mucilage to treat textile effluents, with 67.66%% COD removal, 96.86% turbidity removal and 91.12% apparent color reduction.
31746805	4	64	theme	COD	665:667	arg1	removal					670:676	chemical oxygen demand (COD) removal	641:676	chemical oxygen demand (COD) removal	641:676	We were able to analyze the synergistic effects between the FeCl3.6H2O and mucilage concentrations: the process of coagulation/flocculation (CF) for chemical oxygen demand (COD) removal was more efficient at a low chemical coagulant concentration (8.00 mg L-1) and a higher natural coagulant (NC) concentration (15.00 mg L-1).
31746805	6	65	theme	CF	1321:1322	arg1	process					1324:1330	the CF process	1317:1330	the CF process	1317:1330	The characterization of the mucilage of Dillenia indica was done using Fourier transform infrared spectroscopy (FTIR) and solid-state cross-polarization magic angle spinning carbon-13 nuclear magnetic resonance (CP/MAS 13C NMR), and the signals obtained indicated the presence of polysaccharides, which are responsible for enhancing the CF process.
31746805	6	66	theme	cross-polarization	1118:1135	arg1	angle					1143:1147	solid-state cross-polarization magic angle	1106:1147	solid-state cross-polarization magic angle spinning carbon-13 nuclear magnetic resonance (CP/MAS 13C NMR)	1106:1210	The characterization of the mucilage of Dillenia indica was done using Fourier transform infrared spectroscopy (FTIR) and solid-state cross-polarization magic angle spinning carbon-13 nuclear magnetic resonance (CP/MAS 13C NMR), and the signals obtained indicated the presence of polysaccharides, which are responsible for enhancing the CF process.
33499919	15	0	from	changes	2243:2249	arg1	composition					2279:2289	the mouse gut microbiota composition	2254:2289	the mouse gut microbiota composition	2254:2289	CONCLUSIONS Leptin deficiency caused major changes in the mouse gut microbiota composition.
33499919	13	1	theme	ABC	1959:1961	arg1	transporters					1963:1974	ABC transporters	1959:1974	ABC transporters	1959:1974	Microbial metabolic functions related to energy harvest, including glycan degradation, phosphotransferase systems and ABC transporters, were enriched in the ob/ob mice.
33499919	13	1	theme	ABC	1959:1961	arg1	degradation					1915:1925	glycan degradation	1908:1925	glycan degradation	1908:1925	Microbial metabolic functions related to energy harvest, including glycan degradation, phosphotransferase systems and ABC transporters, were enriched in the ob/ob mice.
33499919	13	1	theme	ABC	1959:1961	arg1	functions					1861:1869	Microbial metabolic functions	1841:1869	Microbial metabolic functions related to energy harvest	1841:1895	Microbial metabolic functions related to energy harvest, including glycan degradation, phosphotransferase systems and ABC transporters, were enriched in the ob/ob mice.
33499919	17	2	theme	obese	2562:2566	arg1	individuals					2568:2578	genetically obese individuals	2550:2578	genetically obese individuals	2550:2578	Pair-fed mice maintained a pre-set high proportion of body fat despite reduced calorie intake, and exhibited more compromised glucose metabolism, with major implications for treatment options for genetically obese individuals.
33499919	17	3	theme	high	2389:2392	arg1	proportion					2394:2403	a pre-set high proportion	2379:2403	a pre-set high proportion of body fat	2379:2415	Pair-fed mice maintained a pre-set high proportion of body fat despite reduced calorie intake, and exhibited more compromised glucose metabolism, with major implications for treatment options for genetically obese individuals.
33499919	2	4	theme	leptin	267:272	arg1	deficiency					274:283	leptin deficiency	267:283	leptin deficiency	267:283	Ob/ob mice, which are hyperphagic due to leptin deficiency, are commonly used models of obesity and were instrumental in suggesting links between gut microbiota and obesity.
33499919	12	5	with	depletion	1803:1811	arg1	deficiency					1740:1749	leptin deficiency	1733:1749	leptin deficiency	1733:1749	We found 24 microbial taxa associations with leptin deficiency, notably enrichment of members of Lactobacillus and depletion of Akkermansia muciniphila.
33499919	12	6	theme	leptin	1733:1738	arg1	deficiency					1740:1749	leptin deficiency	1733:1749	leptin deficiency	1733:1749	We found 24 microbial taxa associations with leptin deficiency, notably enrichment of members of Lactobacillus and depletion of Akkermansia muciniphila.
33499919	4	7	theme	ob/ob	666:670	arg1	microbiota					682:691	ob/ob mouse gut microbiota	666:691	ob/ob mouse gut microbiota	666:691	However, the differential development of ob/ob mouse gut microbiota compared to wild type microbiota and the role of hyperphagia in their metabolic impairment have not been investigated thoroughly.
33499919	0	8	theme	Impaired	0:7	arg1	metabolism					17:26	Impaired glucose metabolism	0:26	Impaired glucose metabolism	0:26	Impaired glucose metabolism and altered gut microbiome despite calorie restriction of ob/ob mice.
33499919	5	9	theme	ob/ob	868:872	arg1	mice					914:917	ob/ob (n = 12) and wild type control (n = 12) mice	868:917	ob/ob (n = 12) and wild type control (n = 12) mice fed ad libitum	868:932	RESULTS We performed a 10-week long study in ob/ob (n = 12) and wild type control (n = 12) mice fed ad libitum.
33499919	17	10	theme	compromised	2468:2478	arg1	metabolism					2488:2497	more compromised glucose metabolism	2463:2497	more compromised glucose metabolism	2463:2497	Pair-fed mice maintained a pre-set high proportion of body fat despite reduced calorie intake, and exhibited more compromised glucose metabolism, with major implications for treatment options for genetically obese individuals.
33499919	4	11	theme	gut	678:680	arg1	microbiota					682:691	ob/ob mouse gut microbiota	666:691	ob/ob mouse gut microbiota	666:691	However, the differential development of ob/ob mouse gut microbiota compared to wild type microbiota and the role of hyperphagia in their metabolic impairment have not been investigated thoroughly.
33499919	4	12	from	development	651:661	arg1	impairment					773:782	their metabolic impairment	757:782	their metabolic impairment	757:782	However, the differential development of ob/ob mouse gut microbiota compared to wild type microbiota and the role of hyperphagia in their metabolic impairment have not been investigated thoroughly.
33499919	1	13	theme	related	181:187	arg1	diabetes					216:223	type 2 diabetes	209:223	type 2 diabetes	209:223	BACKGROUND Growing evidence supports the role of gut microbiota in obesity and its related disorders including type 2 diabetes.
33499919	1	13	theme	related	181:187	arg1	disorders					189:197	its related disorders	177:197	its related disorders including type 2 diabetes	177:223	BACKGROUND Growing evidence supports the role of gut microbiota in obesity and its related disorders including type 2 diabetes.
33499919	12	14	theme	microbial	1700:1708	arg1	associations					1715:1726	24 microbial taxa associations	1697:1726	24 microbial taxa associations with leptin deficiency	1697:1749	We found 24 microbial taxa associations with leptin deficiency, notably enrichment of members of Lactobacillus and depletion of Akkermansia muciniphila.
33499919	5	15	theme	10-week	846:852	arg1	study					859:863	a 10-week long study	844:863	a 10-week long study in ob/ob (n = 12) and wild type control (n = 12) mice fed ad libitum	844:932	RESULTS We performed a 10-week long study in ob/ob (n = 12) and wild type control (n = 12) mice fed ad libitum.
33499919	8	16	theme	ob/ob	1238:1242	arg1	mice					1244:1247	Pair-fed ob/ob mice	1229:1247	Pair-fed ob/ob mice	1229:1247	Pair-fed ob/ob mice exhibited even more compromised glucose metabolism and maintained strikingly similar high body fat percentage at the cost of lean body mass.
33499919	2	17	theme	Ob/ob	226:230	arg1	models					304:309	commonly used models	290:309	commonly used models of obesity	290:320	Ob/ob mice, which are hyperphagic due to leptin deficiency, are commonly used models of obesity and were instrumental in suggesting links between gut microbiota and obesity.
33499919	2	17	theme	Ob/ob	226:230	arg1	mice					232:235	Ob/ob mice	226:235	Ob/ob mice	226:235	Ob/ob mice, which are hyperphagic due to leptin deficiency, are commonly used models of obesity and were instrumental in suggesting links between gut microbiota and obesity.
33499919	3	18	theme	microbiota	586:595	arg1	capacity					597:604	increased microbiota capacity	576:604	increased microbiota capacity to harvest energy	576:622	Specific changes in their gut microbiota such as decreased microbial diversity and increased Firmicutes to Bacteroidetes ratio have been suggested to contribute to obesity via increased microbiota capacity to harvest energy.
33499919	15	19	theme	major	2237:2241	arg1	changes					2243:2249	major changes	2237:2249	major changes in the mouse gut microbiota composition	2237:2289	CONCLUSIONS Leptin deficiency caused major changes in the mouse gut microbiota composition.
33499919	4	20	theme	microbiota	682:691	arg1	role					734:737	the role	730:737	the role of hyperphagia in their metabolic impairment	730:782	However, the differential development of ob/ob mouse gut microbiota compared to wild type microbiota and the role of hyperphagia in their metabolic impairment have not been investigated thoroughly.
33499919	4	20	theme	microbiota	682:691	arg1	development					651:661	the differential development	634:661	the differential development of ob/ob mouse gut microbiota compared to wild type microbiota	634:724	However, the differential development of ob/ob mouse gut microbiota compared to wild type microbiota and the role of hyperphagia in their metabolic impairment have not been investigated thoroughly.
33499919	8	21	theme	more	1264:1267	arg1	metabolism					1289:1298	even more compromised glucose metabolism	1259:1298	even more compromised glucose metabolism	1259:1298	Pair-fed ob/ob mice exhibited even more compromised glucose metabolism and maintained strikingly similar high body fat percentage at the cost of lean body mass.
33499919	5	22	theme	ad	923:924	arg1	libitum					926:932	ad libitum	923:932	ad libitum	923:932	RESULTS We performed a 10-week long study in ob/ob (n = 12) and wild type control (n = 12) mice fed ad libitum.
33499919	12	23	with	associations	1715:1726	arg1	deficiency					1740:1749	leptin deficiency	1733:1749	leptin deficiency	1733:1749	We found 24 microbial taxa associations with leptin deficiency, notably enrichment of members of Lactobacillus and depletion of Akkermansia muciniphila.
33499919	15	24	theme	Leptin	2212:2217	arg1	deficiency					2219:2228	CONCLUSIONS Leptin deficiency	2200:2228	CONCLUSIONS Leptin deficiency	2200:2228	CONCLUSIONS Leptin deficiency caused major changes in the mouse gut microbiota composition.
33499919	8	25	theme	glucose	1281:1287	arg1	metabolism					1289:1298	even more compromised glucose metabolism	1259:1298	even more compromised glucose metabolism	1259:1298	Pair-fed ob/ob mice exhibited even more compromised glucose metabolism and maintained strikingly similar high body fat percentage at the cost of lean body mass.
33499919	16	26	theme	Several	2292:2298	arg1	taxa					2310:2313	Several microbial taxa	2292:2313	Several microbial taxa	2292:2313	Several microbial taxa were associated with body composition.
33499919	0	27	theme	calorie	63:69	arg1	restriction					71:81	calorie restriction	63:81	calorie restriction of ob/ob mice	63:95	Impaired glucose metabolism and altered gut microbiome despite calorie restriction of ob/ob mice.
33499919	1	28	theme	gut	147:149	arg1	microbiota					151:160	gut microbiota	147:160	gut microbiota	147:160	BACKGROUND Growing evidence supports the role of gut microbiota in obesity and its related disorders including type 2 diabetes.
33499919	4	29	theme	wild	705:708	arg1	microbiota					715:724	wild type microbiota	705:724	wild type microbiota	705:724	However, the differential development of ob/ob mouse gut microbiota compared to wild type microbiota and the role of hyperphagia in their metabolic impairment have not been investigated thoroughly.
33499919	7	30	theme	compromised	1163:1173	arg1	metabolism					1183:1192	compromised glucose metabolism	1163:1192	compromised glucose metabolism	1163:1192	Compared to control mice, ob/ob mice fed ad libitum exhibited compromised glucose metabolism and increased body fat percentage.
33499919	5	31	theme	type	892:895	arg1	mice					914:917	ob/ob (n = 12) and wild type control (n = 12) mice	868:917	ob/ob (n = 12) and wild type control (n = 12) mice fed ad libitum	868:932	RESULTS We performed a 10-week long study in ob/ob (n = 12) and wild type control (n = 12) mice fed ad libitum.
33499919	6	32	theme	deficiency	970:979	arg1	effects					952:958	effects	952:958	effects of leptin deficiency from hyperphagia	952:996	To differentiate effects of leptin deficiency from hyperphagia, we pair-fed an additional group of ob/ob mice (n = 11) based on the food consumption of control mice.
33499919	15	33	theme	CONCLUSIONS	2200:2210	arg1	deficiency					2219:2228	CONCLUSIONS Leptin deficiency	2200:2228	CONCLUSIONS Leptin deficiency	2200:2228	CONCLUSIONS Leptin deficiency caused major changes in the mouse gut microbiota composition.
33499919	4	34	theme	metabolic	763:771	arg1	impairment					773:782	their metabolic impairment	757:782	their metabolic impairment	757:782	However, the differential development of ob/ob mouse gut microbiota compared to wild type microbiota and the role of hyperphagia in their metabolic impairment have not been investigated thoroughly.
33499919	16	35	theme	body	2336:2339	arg1	composition					2341:2351	body composition	2336:2351	body composition	2336:2351	Several microbial taxa were associated with body composition.
33499919	0	36	theme	mice	92:95	arg1	restriction					71:81	calorie restriction	63:81	calorie restriction of ob/ob mice	63:95	Impaired glucose metabolism and altered gut microbiome despite calorie restriction of ob/ob mice.
33499919	8	37	theme	high	1334:1337	arg1	percentage					1348:1357	strikingly similar high body fat percentage	1315:1357	strikingly similar high body fat percentage	1315:1357	Pair-fed ob/ob mice exhibited even more compromised glucose metabolism and maintained strikingly similar high body fat percentage at the cost of lean body mass.
33499919	13	38	theme	Microbial	1841:1849	arg1	systems					1947:1953	phosphotransferase systems	1928:1953	phosphotransferase systems	1928:1953	Microbial metabolic functions related to energy harvest, including glycan degradation, phosphotransferase systems and ABC transporters, were enriched in the ob/ob mice.
33499919	13	38	theme	Microbial	1841:1849	arg1	transporters					1963:1974	ABC transporters	1959:1974	ABC transporters	1959:1974	Microbial metabolic functions related to energy harvest, including glycan degradation, phosphotransferase systems and ABC transporters, were enriched in the ob/ob mice.
33499919	13	38	theme	Microbial	1841:1849	arg1	degradation					1915:1925	glycan degradation	1908:1925	glycan degradation	1908:1925	Microbial metabolic functions related to energy harvest, including glycan degradation, phosphotransferase systems and ABC transporters, were enriched in the ob/ob mice.
33499919	13	38	theme	Microbial	1841:1849	arg1	functions					1861:1869	Microbial metabolic functions	1841:1869	Microbial metabolic functions related to energy harvest	1841:1895	Microbial metabolic functions related to energy harvest, including glycan degradation, phosphotransferase systems and ABC transporters, were enriched in the ob/ob mice.
33499919	8	39	theme	fat	1344:1346	arg1	percentage					1348:1357	strikingly similar high body fat percentage	1315:1357	strikingly similar high body fat percentage	1315:1357	Pair-fed ob/ob mice exhibited even more compromised glucose metabolism and maintained strikingly similar high body fat percentage at the cost of lean body mass.
33499919	7	40	theme	ob/ob	1127:1131	arg1	mice					1133:1136	ob/ob mice	1127:1136	ob/ob mice fed ad libitum	1127:1151	Compared to control mice, ob/ob mice fed ad libitum exhibited compromised glucose metabolism and increased body fat percentage.
33499919	6	41	theme	ob/ob	1034:1038	arg1	mice					1040:1043	ob/ob mice	1034:1043	ob/ob mice (n = 11)	1034:1052	To differentiate effects of leptin deficiency from hyperphagia, we pair-fed an additional group of ob/ob mice (n = 11) based on the food consumption of control mice.
33499919	6	41	theme	ob/ob	1034:1038	arg1	n = 11					1046:1051	n = 11	1046:1051	n = 11	1046:1051	To differentiate effects of leptin deficiency from hyperphagia, we pair-fed an additional group of ob/ob mice (n = 11) based on the food consumption of control mice.
33499919	11	42	from	end	1638:1640	arg1	different					1677:1685	different	1677:1685	different	1677:1685	Pair-feeding also altered several taxa, although the overall community composition at the end of the study was not significantly different.
33499919	11	42	from	end	1638:1640	arg1	composition					1619:1629	the overall community composition	1597:1629	the overall community composition at the end of the study	1597:1653	Pair-feeding also altered several taxa, although the overall community composition at the end of the study was not significantly different.
33499919	17	43	theme	body	2408:2411	arg1	fat					2413:2415	body fat	2408:2415	body fat	2408:2415	Pair-fed mice maintained a pre-set high proportion of body fat despite reduced calorie intake, and exhibited more compromised glucose metabolism, with major implications for treatment options for genetically obese individuals.
33499919	10	44	theme	Leptin	1460:1465	arg1	deficiency					1467:1476	Leptin deficiency	1460:1476	Leptin deficiency	1460:1476	Leptin deficiency impacted gut microbial composition, explaining 18.3% of the variance.
33499919	15	45	theme	gut	2264:2266	arg1	composition					2279:2289	the mouse gut microbiota composition	2254:2289	the mouse gut microbiota composition	2254:2289	CONCLUSIONS Leptin deficiency caused major changes in the mouse gut microbiota composition.
33499919	1	46	theme	Growing	109:115	arg1	evidence					117:124	BACKGROUND Growing evidence	98:124	BACKGROUND Growing evidence	98:124	BACKGROUND Growing evidence supports the role of gut microbiota in obesity and its related disorders including type 2 diabetes.
33499919	14	47	dep	Prevotella	2164:2173	arg1	correlated					2187:2196	correlated	2187:2196	correlated	2187:2196	Taxa previously reported as relevant for obesity were associated with body weight, including Oscillibacter and Alistipes (both negatively correlated) and Prevotella (positively correlated).
33499919	0	48	theme	altered	32:38	arg1	gut					40:42	altered gut	32:42	altered gut	32:42	Impaired glucose metabolism and altered gut microbiome despite calorie restriction of ob/ob mice.
33499919	17	49	theme	calorie	2433:2439	arg1	intake					2441:2446	reduced calorie intake	2425:2446	reduced calorie intake	2425:2446	Pair-fed mice maintained a pre-set high proportion of body fat despite reduced calorie intake, and exhibited more compromised glucose metabolism, with major implications for treatment options for genetically obese individuals.
33499919	10	50	theme	microbial	1491:1499	arg1	composition					1501:1511	gut microbial composition	1487:1511	gut microbial composition	1487:1511	Leptin deficiency impacted gut microbial composition, explaining 18.3% of the variance.
33499919	11	51	theme	study	1649:1653	arg1	end					1638:1640	the end	1634:1640	the end of the study	1634:1653	Pair-feeding also altered several taxa, although the overall community composition at the end of the study was not significantly different.
33499919	8	52	theme	lean	1374:1377	arg1	mass					1384:1387	lean body mass	1374:1387	lean body mass	1374:1387	Pair-fed ob/ob mice exhibited even more compromised glucose metabolism and maintained strikingly similar high body fat percentage at the cost of lean body mass.
33499919	17	53	theme	major	2505:2509	arg1	implications					2511:2522	major implications	2505:2522	major implications for treatment options for genetically obese individuals	2505:2578	Pair-fed mice maintained a pre-set high proportion of body fat despite reduced calorie intake, and exhibited more compromised glucose metabolism, with major implications for treatment options for genetically obese individuals.
33499919	8	54	theme	mass	1384:1387	arg1	cost					1366:1369	the cost	1362:1369	the cost of lean body mass	1362:1387	Pair-fed ob/ob mice exhibited even more compromised glucose metabolism and maintained strikingly similar high body fat percentage at the cost of lean body mass.
33499919	3	55	theme	microbial	459:467	arg1	diversity					469:477	decreased microbial diversity	449:477	decreased microbial diversity	449:477	Specific changes in their gut microbiota such as decreased microbial diversity and increased Firmicutes to Bacteroidetes ratio have been suggested to contribute to obesity via increased microbiota capacity to harvest energy.
33499919	12	56	theme	Akkermansia	1816:1826	arg1	muciniphila					1828:1838	Akkermansia muciniphila	1816:1838	Akkermansia muciniphila	1816:1838	We found 24 microbial taxa associations with leptin deficiency, notably enrichment of members of Lactobacillus and depletion of Akkermansia muciniphila.
33499919	6	57	theme	food	1067:1070	arg1	consumption					1072:1082	the food consumption	1063:1082	the food consumption of control mice	1063:1098	To differentiate effects of leptin deficiency from hyperphagia, we pair-fed an additional group of ob/ob mice (n = 11) based on the food consumption of control mice.
33499919	17	58	theme	Pair-fed	2354:2361	arg1	mice					2363:2366	Pair-fed mice	2354:2366	Pair-fed mice	2354:2366	Pair-fed mice maintained a pre-set high proportion of body fat despite reduced calorie intake, and exhibited more compromised glucose metabolism, with major implications for treatment options for genetically obese individuals.
33499919	3	59	theme	Bacteroidetes	507:519	arg1	ratio					521:525	Bacteroidetes ratio	507:525	Bacteroidetes ratio	507:525	Specific changes in their gut microbiota such as decreased microbial diversity and increased Firmicutes to Bacteroidetes ratio have been suggested to contribute to obesity via increased microbiota capacity to harvest energy.
33499919	7	60	theme	fat	1213:1215	arg1	percentage					1217:1226	increased body fat percentage	1198:1226	increased body fat percentage	1198:1226	Compared to control mice, ob/ob mice fed ad libitum exhibited compromised glucose metabolism and increased body fat percentage.
33499919	13	61	theme	ob/ob	1998:2002	arg1	mice					2004:2007	the ob/ob mice	1994:2007	the ob/ob mice	1994:2007	Microbial metabolic functions related to energy harvest, including glycan degradation, phosphotransferase systems and ABC transporters, were enriched in the ob/ob mice.
33499919	7	62	theme	increased	1198:1206	arg1	percentage					1217:1226	increased body fat percentage	1198:1226	increased body fat percentage	1198:1226	Compared to control mice, ob/ob mice fed ad libitum exhibited compromised glucose metabolism and increased body fat percentage.
33499919	17	63	theme	pre-set	2381:2387	arg1	proportion					2394:2403	a pre-set high proportion	2379:2403	a pre-set high proportion of body fat	2379:2415	Pair-fed mice maintained a pre-set high proportion of body fat despite reduced calorie intake, and exhibited more compromised glucose metabolism, with major implications for treatment options for genetically obese individuals.
33499919	10	64	theme	variance	1538:1545	arg1	%					1529:1529	18.3%	1525:1529	18.3% of the variance	1525:1545	Leptin deficiency impacted gut microbial composition, explaining 18.3% of the variance.
33499919	10	64	theme	variance	1538:1545	arg1	variance					1538:1545	the variance	1534:1545	the variance	1534:1545	Leptin deficiency impacted gut microbial composition, explaining 18.3% of the variance.
33499919	11	65	theme	overall	1601:1607	arg1	different					1677:1685	different	1677:1685	different	1677:1685	Pair-feeding also altered several taxa, although the overall community composition at the end of the study was not significantly different.
33499919	11	65	theme	overall	1601:1607	arg1	composition					1619:1629	the overall community composition	1597:1629	the overall community composition at the end of the study	1597:1653	Pair-feeding also altered several taxa, although the overall community composition at the end of the study was not significantly different.
33499919	5	66	dep	RESULTS	823:829	arg1	performed					834:842	performed	834:842	performed a 10-week long study in ob/ob (n = 12) and wild type control (n = 12) mice fed ad libitum	834:932	RESULTS We performed a 10-week long study in ob/ob (n = 12) and wild type control (n = 12) mice fed ad libitum.
33499919	5	67	theme	wild	887:890	arg1	type					892:895	wild type	887:895	wild type	887:895	RESULTS We performed a 10-week long study in ob/ob (n = 12) and wild type control (n = 12) mice fed ad libitum.
33499919	14	68	dep	Oscillibacter	2103:2115	arg1	correlated					2148:2157	correlated	2148:2157	correlated	2148:2157	Taxa previously reported as relevant for obesity were associated with body weight, including Oscillibacter and Alistipes (both negatively correlated) and Prevotella (positively correlated).
33499919	13	69	theme	phosphotransferase	1928:1945	arg1	systems					1947:1953	phosphotransferase systems	1928:1953	phosphotransferase systems	1928:1953	Microbial metabolic functions related to energy harvest, including glycan degradation, phosphotransferase systems and ABC transporters, were enriched in the ob/ob mice.
33499919	13	69	theme	phosphotransferase	1928:1945	arg1	degradation					1915:1925	glycan degradation	1908:1925	glycan degradation	1908:1925	Microbial metabolic functions related to energy harvest, including glycan degradation, phosphotransferase systems and ABC transporters, were enriched in the ob/ob mice.
33499919	13	69	theme	phosphotransferase	1928:1945	arg1	functions					1861:1869	Microbial metabolic functions	1841:1869	Microbial metabolic functions related to energy harvest	1841:1895	Microbial metabolic functions related to energy harvest, including glycan degradation, phosphotransferase systems and ABC transporters, were enriched in the ob/ob mice.
33499919	2	70	theme	used	299:302	arg1	models					304:309	commonly used models	290:309	commonly used models of obesity	290:320	Ob/ob mice, which are hyperphagic due to leptin deficiency, are commonly used models of obesity and were instrumental in suggesting links between gut microbiota and obesity.
33499919	2	70	theme	used	299:302	arg1	mice					232:235	Ob/ob mice	226:235	Ob/ob mice	226:235	Ob/ob mice, which are hyperphagic due to leptin deficiency, are commonly used models of obesity and were instrumental in suggesting links between gut microbiota and obesity.
33499919	9	71	theme	microbiota	1413:1422	arg1	Acclimatization					1390:1404	Acclimatization	1390:1404	Acclimatization of the microbiota to our facility	1390:1438	Acclimatization of the microbiota to our facility took up to 5 weeks.
33499919	12	72	theme	taxa	1710:1713	arg1	associations					1715:1726	24 microbial taxa associations	1697:1726	24 microbial taxa associations with leptin deficiency	1697:1749	We found 24 microbial taxa associations with leptin deficiency, notably enrichment of members of Lactobacillus and depletion of Akkermansia muciniphila.
33499919	17	73	theme	glucose	2480:2486	arg1	metabolism					2488:2497	more compromised glucose metabolism	2463:2497	more compromised glucose metabolism	2463:2497	Pair-fed mice maintained a pre-set high proportion of body fat despite reduced calorie intake, and exhibited more compromised glucose metabolism, with major implications for treatment options for genetically obese individuals.
33499919	4	74	theme	mouse	672:676	arg1	microbiota					682:691	ob/ob mouse gut microbiota	666:691	ob/ob mouse gut microbiota	666:691	However, the differential development of ob/ob mouse gut microbiota compared to wild type microbiota and the role of hyperphagia in their metabolic impairment have not been investigated thoroughly.
33499919	6	75	theme	mice	1095:1098	arg1	consumption					1072:1082	the food consumption	1063:1082	the food consumption of control mice	1063:1098	To differentiate effects of leptin deficiency from hyperphagia, we pair-fed an additional group of ob/ob mice (n = 11) based on the food consumption of control mice.
33499919	2	76	theme	obesity	314:320	arg1	models					304:309	commonly used models	290:309	commonly used models of obesity	290:320	Ob/ob mice, which are hyperphagic due to leptin deficiency, are commonly used models of obesity and were instrumental in suggesting links between gut microbiota and obesity.
33499919	2	76	theme	obesity	314:320	arg1	mice					232:235	Ob/ob mice	226:235	Ob/ob mice	226:235	Ob/ob mice, which are hyperphagic due to leptin deficiency, are commonly used models of obesity and were instrumental in suggesting links between gut microbiota and obesity.
33499919	5	77	theme	long	854:857	arg1	study					859:863	a 10-week long study	844:863	a 10-week long study in ob/ob (n = 12) and wild type control (n = 12) mice fed ad libitum	844:932	RESULTS We performed a 10-week long study in ob/ob (n = 12) and wild type control (n = 12) mice fed ad libitum.
33499919	4	78	theme	differential	638:649	arg1	development					651:661	the differential development	634:661	the differential development of ob/ob mouse gut microbiota compared to wild type microbiota	634:724	However, the differential development of ob/ob mouse gut microbiota compared to wild type microbiota and the role of hyperphagia in their metabolic impairment have not been investigated thoroughly.
33499919	3	79	theme	increased	576:584	arg1	capacity					597:604	increased microbiota capacity	576:604	increased microbiota capacity to harvest energy	576:622	Specific changes in their gut microbiota such as decreased microbial diversity and increased Firmicutes to Bacteroidetes ratio have been suggested to contribute to obesity via increased microbiota capacity to harvest energy.
33499919	5	80	from	study	859:863	arg1	mice					914:917	ob/ob (n = 12) and wild type control (n = 12) mice	868:917	ob/ob (n = 12) and wild type control (n = 12) mice fed ad libitum	868:932	RESULTS We performed a 10-week long study in ob/ob (n = 12) and wild type control (n = 12) mice fed ad libitum.
33499919	5	81	theme	control	897:903	arg1	mice					914:917	ob/ob (n = 12) and wild type control (n = 12) mice	868:917	ob/ob (n = 12) and wild type control (n = 12) mice fed ad libitum	868:932	RESULTS We performed a 10-week long study in ob/ob (n = 12) and wild type control (n = 12) mice fed ad libitum.
33499919	4	82	theme	type	710:713	arg1	microbiota					715:724	wild type microbiota	705:724	wild type microbiota	705:724	However, the differential development of ob/ob mouse gut microbiota compared to wild type microbiota and the role of hyperphagia in their metabolic impairment have not been investigated thoroughly.
33499919	16	83	theme	microbial	2300:2308	arg1	taxa					2310:2313	Several microbial taxa	2292:2313	Several microbial taxa	2292:2313	Several microbial taxa were associated with body composition.
33499919	5	84	theme	n = 12	906:911	arg1	mice					914:917	ob/ob (n = 12) and wild type control (n = 12) mice	868:917	ob/ob (n = 12) and wild type control (n = 12) mice fed ad libitum	868:932	RESULTS We performed a 10-week long study in ob/ob (n = 12) and wild type control (n = 12) mice fed ad libitum.
33499919	13	85	theme	glycan	1908:1913	arg1	degradation					1915:1925	glycan degradation	1908:1925	glycan degradation	1908:1925	Microbial metabolic functions related to energy harvest, including glycan degradation, phosphotransferase systems and ABC transporters, were enriched in the ob/ob mice.
33499919	7	86	theme	glucose	1175:1181	arg1	metabolism					1183:1192	compromised glucose metabolism	1163:1192	compromised glucose metabolism	1163:1192	Compared to control mice, ob/ob mice fed ad libitum exhibited compromised glucose metabolism and increased body fat percentage.
33499919	3	87	theme	harvest	609:615	arg1	energy					617:622	harvest energy	609:622	harvest energy	609:622	Specific changes in their gut microbiota such as decreased microbial diversity and increased Firmicutes to Bacteroidetes ratio have been suggested to contribute to obesity via increased microbiota capacity to harvest energy.
33499919	8	88	theme	compromised	1269:1279	arg1	metabolism					1289:1298	even more compromised glucose metabolism	1259:1298	even more compromised glucose metabolism	1259:1298	Pair-fed ob/ob mice exhibited even more compromised glucose metabolism and maintained strikingly similar high body fat percentage at the cost of lean body mass.
33499919	13	89	theme	related	1871:1877	arg1	systems					1947:1953	phosphotransferase systems	1928:1953	phosphotransferase systems	1928:1953	Microbial metabolic functions related to energy harvest, including glycan degradation, phosphotransferase systems and ABC transporters, were enriched in the ob/ob mice.
33499919	13	89	theme	related	1871:1877	arg1	transporters					1963:1974	ABC transporters	1959:1974	ABC transporters	1959:1974	Microbial metabolic functions related to energy harvest, including glycan degradation, phosphotransferase systems and ABC transporters, were enriched in the ob/ob mice.
33499919	13	89	theme	related	1871:1877	arg1	degradation					1915:1925	glycan degradation	1908:1925	glycan degradation	1908:1925	Microbial metabolic functions related to energy harvest, including glycan degradation, phosphotransferase systems and ABC transporters, were enriched in the ob/ob mice.
33499919	13	89	theme	related	1871:1877	arg1	functions					1861:1869	Microbial metabolic functions	1841:1869	Microbial metabolic functions related to energy harvest	1841:1895	Microbial metabolic functions related to energy harvest, including glycan degradation, phosphotransferase systems and ABC transporters, were enriched in the ob/ob mice.
33499919	0	90	theme	ob/ob	86:90	arg1	mice					92:95	ob/ob mice	86:95	ob/ob mice	86:95	Impaired glucose metabolism and altered gut microbiome despite calorie restriction of ob/ob mice.
33499919	1	91	theme	microbiota	151:160	arg1	role					139:142	the role	135:142	the role of gut microbiota in obesity and its related disorders including type 2 diabetes	135:223	BACKGROUND Growing evidence supports the role of gut microbiota in obesity and its related disorders including type 2 diabetes.
33499919	6	92	from	hyperphagia	986:996	arg1	effects					952:958	effects	952:958	effects of leptin deficiency from hyperphagia	952:996	To differentiate effects of leptin deficiency from hyperphagia, we pair-fed an additional group of ob/ob mice (n = 11) based on the food consumption of control mice.
33499919	13	93	theme	energy	1882:1887	arg1	harvest					1889:1895	energy harvest	1882:1895	energy harvest	1882:1895	Microbial metabolic functions related to energy harvest, including glycan degradation, phosphotransferase systems and ABC transporters, were enriched in the ob/ob mice.
33499919	7	94	theme	ad	1142:1143	arg1	libitum					1145:1151	ad libitum	1142:1151	ad libitum	1142:1151	Compared to control mice, ob/ob mice fed ad libitum exhibited compromised glucose metabolism and increased body fat percentage.
33499919	6	95	theme	leptin	963:968	arg1	deficiency					970:979	leptin deficiency	963:979	leptin deficiency	963:979	To differentiate effects of leptin deficiency from hyperphagia, we pair-fed an additional group of ob/ob mice (n = 11) based on the food consumption of control mice.
33499919	12	96	with	enrichment	1760:1769	arg1	deficiency					1740:1749	leptin deficiency	1733:1749	leptin deficiency	1733:1749	We found 24 microbial taxa associations with leptin deficiency, notably enrichment of members of Lactobacillus and depletion of Akkermansia muciniphila.
33499919	8	97	theme	similar	1326:1332	arg1	percentage					1348:1357	strikingly similar high body fat percentage	1315:1357	strikingly similar high body fat percentage	1315:1357	Pair-fed ob/ob mice exhibited even more compromised glucose metabolism and maintained strikingly similar high body fat percentage at the cost of lean body mass.
33499919	13	98	theme	metabolic	1851:1859	arg1	systems					1947:1953	phosphotransferase systems	1928:1953	phosphotransferase systems	1928:1953	Microbial metabolic functions related to energy harvest, including glycan degradation, phosphotransferase systems and ABC transporters, were enriched in the ob/ob mice.
33499919	13	98	theme	metabolic	1851:1859	arg1	transporters					1963:1974	ABC transporters	1959:1974	ABC transporters	1959:1974	Microbial metabolic functions related to energy harvest, including glycan degradation, phosphotransferase systems and ABC transporters, were enriched in the ob/ob mice.
33499919	13	98	theme	metabolic	1851:1859	arg1	degradation					1915:1925	glycan degradation	1908:1925	glycan degradation	1908:1925	Microbial metabolic functions related to energy harvest, including glycan degradation, phosphotransferase systems and ABC transporters, were enriched in the ob/ob mice.
33499919	13	98	theme	metabolic	1851:1859	arg1	functions					1861:1869	Microbial metabolic functions	1841:1869	Microbial metabolic functions related to energy harvest	1841:1895	Microbial metabolic functions related to energy harvest, including glycan degradation, phosphotransferase systems and ABC transporters, were enriched in the ob/ob mice.
33499919	2	99	theme	gut	372:374	arg1	microbiota					376:385	gut microbiota	372:385	gut microbiota	372:385	Ob/ob mice, which are hyperphagic due to leptin deficiency, are commonly used models of obesity and were instrumental in suggesting links between gut microbiota and obesity.
33499919	0	100	theme	glucose	9:15	arg1	metabolism					17:26	Impaired glucose metabolism	0:26	Impaired glucose metabolism	0:26	Impaired glucose metabolism and altered gut microbiome despite calorie restriction of ob/ob mice.
33499919	1	101	from	role	139:142	arg1	obesity					165:171	obesity	165:171	obesity	165:171	BACKGROUND Growing evidence supports the role of gut microbiota in obesity and its related disorders including type 2 diabetes.
33499919	1	101	from	role	139:142	arg1	diabetes					216:223	type 2 diabetes	209:223	type 2 diabetes	209:223	BACKGROUND Growing evidence supports the role of gut microbiota in obesity and its related disorders including type 2 diabetes.
33499919	1	101	from	role	139:142	arg1	disorders					189:197	its related disorders	177:197	its related disorders including type 2 diabetes	177:223	BACKGROUND Growing evidence supports the role of gut microbiota in obesity and its related disorders including type 2 diabetes.
33499919	6	102	theme	mice	1040:1043	arg1	group					1025:1029	an additional group	1011:1029	an additional group of ob/ob mice (n = 11) based on the food consumption of control mice	1011:1098	To differentiate effects of leptin deficiency from hyperphagia, we pair-fed an additional group of ob/ob mice (n = 11) based on the food consumption of control mice.
33499919	6	102	theme	mice	1040:1043	arg1	mice					1040:1043	ob/ob mice	1034:1043	ob/ob mice (n = 11)	1034:1052	To differentiate effects of leptin deficiency from hyperphagia, we pair-fed an additional group of ob/ob mice (n = 11) based on the food consumption of control mice.
33499919	6	102	theme	mice	1040:1043	arg1	n = 11					1046:1051	n = 11	1046:1051	n = 11	1046:1051	To differentiate effects of leptin deficiency from hyperphagia, we pair-fed an additional group of ob/ob mice (n = 11) based on the food consumption of control mice.
33499919	14	103	theme	body	2080:2083	arg1	weight					2085:2090	body weight	2080:2090	body weight	2080:2090	Taxa previously reported as relevant for obesity were associated with body weight, including Oscillibacter and Alistipes (both negatively correlated) and Prevotella (positively correlated).
33499919	8	104	theme	body	1339:1342	arg1	percentage					1348:1357	strikingly similar high body fat percentage	1315:1357	strikingly similar high body fat percentage	1315:1357	Pair-fed ob/ob mice exhibited even more compromised glucose metabolism and maintained strikingly similar high body fat percentage at the cost of lean body mass.
33499919	7	105	theme	control	1113:1119	arg1	mice					1121:1124	control mice	1113:1124	control mice	1113:1124	Compared to control mice, ob/ob mice fed ad libitum exhibited compromised glucose metabolism and increased body fat percentage.
33499919	4	106	from	role	734:737	arg1	impairment					773:782	their metabolic impairment	757:782	their metabolic impairment	757:782	However, the differential development of ob/ob mouse gut microbiota compared to wild type microbiota and the role of hyperphagia in their metabolic impairment have not been investigated thoroughly.
33499919	1	107	theme	BACKGROUND	98:107	arg1	evidence					117:124	BACKGROUND Growing evidence	98:124	BACKGROUND Growing evidence	98:124	BACKGROUND Growing evidence supports the role of gut microbiota in obesity and its related disorders including type 2 diabetes.
33499919	14	108	dep	correlated	2148:2157	arg1	both					2132:2135	both	2132:2135	both	2132:2135	Taxa previously reported as relevant for obesity were associated with body weight, including Oscillibacter and Alistipes (both negatively correlated) and Prevotella (positively correlated).
33499919	14	108	dep	correlated	2148:2157	arg1	negatively					2137:2146	negatively	2137:2146	negatively	2137:2146	Taxa previously reported as relevant for obesity were associated with body weight, including Oscillibacter and Alistipes (both negatively correlated) and Prevotella (positively correlated).
33499919	17	109	theme	fat	2413:2415	arg1	proportion					2394:2403	a pre-set high proportion	2379:2403	a pre-set high proportion of body fat	2379:2415	Pair-fed mice maintained a pre-set high proportion of body fat despite reduced calorie intake, and exhibited more compromised glucose metabolism, with major implications for treatment options for genetically obese individuals.
33499919	4	110	theme	hyperphagia	742:752	arg1	role					734:737	the role	730:737	the role of hyperphagia in their metabolic impairment	730:782	However, the differential development of ob/ob mouse gut microbiota compared to wild type microbiota and the role of hyperphagia in their metabolic impairment have not been investigated thoroughly.
33499919	4	110	theme	hyperphagia	742:752	arg1	development					651:661	the differential development	634:661	the differential development of ob/ob mouse gut microbiota compared to wild type microbiota	634:724	However, the differential development of ob/ob mouse gut microbiota compared to wild type microbiota and the role of hyperphagia in their metabolic impairment have not been investigated thoroughly.
33499919	3	111	theme	decreased	449:457	arg1	diversity					469:477	decreased microbial diversity	449:477	decreased microbial diversity	449:477	Specific changes in their gut microbiota such as decreased microbial diversity and increased Firmicutes to Bacteroidetes ratio have been suggested to contribute to obesity via increased microbiota capacity to harvest energy.
33499919	15	112	theme	microbiota	2268:2277	arg1	composition					2279:2289	the mouse gut microbiota composition	2254:2289	the mouse gut microbiota composition	2254:2289	CONCLUSIONS Leptin deficiency caused major changes in the mouse gut microbiota composition.
33499919	1	113	theme	type	209:212	arg1	diabetes					216:223	type 2 diabetes	209:223	type 2 diabetes	209:223	BACKGROUND Growing evidence supports the role of gut microbiota in obesity and its related disorders including type 2 diabetes.
33499919	17	114	theme	treatment	2528:2536	arg1	options					2538:2544	treatment options	2528:2544	treatment options for genetically obese individuals	2528:2578	Pair-fed mice maintained a pre-set high proportion of body fat despite reduced calorie intake, and exhibited more compromised glucose metabolism, with major implications for treatment options for genetically obese individuals.
33499919	6	115	theme	additional	1014:1023	arg1	group					1025:1029	an additional group	1011:1029	an additional group of ob/ob mice (n = 11) based on the food consumption of control mice	1011:1098	To differentiate effects of leptin deficiency from hyperphagia, we pair-fed an additional group of ob/ob mice (n = 11) based on the food consumption of control mice.
33499919	6	115	theme	additional	1014:1023	arg1	mice					1040:1043	ob/ob mice	1034:1043	ob/ob mice (n = 11)	1034:1052	To differentiate effects of leptin deficiency from hyperphagia, we pair-fed an additional group of ob/ob mice (n = 11) based on the food consumption of control mice.
33499919	6	115	theme	additional	1014:1023	arg1	n = 11					1046:1051	n = 11	1046:1051	n = 11	1046:1051	To differentiate effects of leptin deficiency from hyperphagia, we pair-fed an additional group of ob/ob mice (n = 11) based on the food consumption of control mice.
33499919	3	116	theme	Specific	400:407	arg1	changes					409:415	Specific changes	400:415	Specific changes in their gut microbiota such as decreased microbial diversity and increased Firmicutes to Bacteroidetes ratio	400:525	Specific changes in their gut microbiota such as decreased microbial diversity and increased Firmicutes to Bacteroidetes ratio have been suggested to contribute to obesity via increased microbiota capacity to harvest energy.
33499919	3	116	theme	Specific	400:407	arg1	diversity					469:477	decreased microbial diversity	449:477	decreased microbial diversity	449:477	Specific changes in their gut microbiota such as decreased microbial diversity and increased Firmicutes to Bacteroidetes ratio have been suggested to contribute to obesity via increased microbiota capacity to harvest energy.
33499919	3	116	theme	Specific	400:407	arg1	Firmicutes					493:502	increased Firmicutes	483:502	increased Firmicutes to Bacteroidetes ratio	483:525	Specific changes in their gut microbiota such as decreased microbial diversity and increased Firmicutes to Bacteroidetes ratio have been suggested to contribute to obesity via increased microbiota capacity to harvest energy.
33499919	17	117	theme	reduced	2425:2431	arg1	intake					2441:2446	reduced calorie intake	2425:2446	reduced calorie intake	2425:2446	Pair-fed mice maintained a pre-set high proportion of body fat despite reduced calorie intake, and exhibited more compromised glucose metabolism, with major implications for treatment options for genetically obese individuals.
33499919	15	118	theme	mouse	2258:2262	arg1	composition					2279:2289	the mouse gut microbiota composition	2254:2289	the mouse gut microbiota composition	2254:2289	CONCLUSIONS Leptin deficiency caused major changes in the mouse gut microbiota composition.
33499919	12	119	theme	members	1774:1780	arg1	enrichment					1760:1769	notably enrichment	1752:1769	notably enrichment of members of Lactobacillus	1752:1797	We found 24 microbial taxa associations with leptin deficiency, notably enrichment of members of Lactobacillus and depletion of Akkermansia muciniphila.
33499919	12	119	theme	members	1774:1780	arg1	depletion					1803:1811	depletion	1803:1811	depletion of Akkermansia muciniphila	1803:1838	We found 24 microbial taxa associations with leptin deficiency, notably enrichment of members of Lactobacillus and depletion of Akkermansia muciniphila.
33499919	12	119	theme	members	1774:1780	arg1	associations					1715:1726	24 microbial taxa associations	1697:1726	24 microbial taxa associations with leptin deficiency	1697:1749	We found 24 microbial taxa associations with leptin deficiency, notably enrichment of members of Lactobacillus and depletion of Akkermansia muciniphila.
33499919	6	120	theme	control	1087:1093	arg1	mice					1095:1098	control mice	1087:1098	control mice	1087:1098	To differentiate effects of leptin deficiency from hyperphagia, we pair-fed an additional group of ob/ob mice (n = 11) based on the food consumption of control mice.
33499919	10	121	theme	gut	1487:1489	arg1	composition					1501:1511	gut microbial composition	1487:1511	gut microbial composition	1487:1511	Leptin deficiency impacted gut microbial composition, explaining 18.3% of the variance.
33499919	3	122	theme	increased	483:491	arg1	Firmicutes					493:502	increased Firmicutes	483:502	increased Firmicutes to Bacteroidetes ratio	483:525	Specific changes in their gut microbiota such as decreased microbial diversity and increased Firmicutes to Bacteroidetes ratio have been suggested to contribute to obesity via increased microbiota capacity to harvest energy.
33499919	12	123	theme	muciniphila	1828:1838	arg1	enrichment					1760:1769	notably enrichment	1752:1769	notably enrichment of members of Lactobacillus	1752:1797	We found 24 microbial taxa associations with leptin deficiency, notably enrichment of members of Lactobacillus and depletion of Akkermansia muciniphila.
33499919	12	123	theme	muciniphila	1828:1838	arg1	depletion					1803:1811	depletion	1803:1811	depletion of Akkermansia muciniphila	1803:1838	We found 24 microbial taxa associations with leptin deficiency, notably enrichment of members of Lactobacillus and depletion of Akkermansia muciniphila.
33499919	12	123	theme	muciniphila	1828:1838	arg1	associations					1715:1726	24 microbial taxa associations	1697:1726	24 microbial taxa associations with leptin deficiency	1697:1749	We found 24 microbial taxa associations with leptin deficiency, notably enrichment of members of Lactobacillus and depletion of Akkermansia muciniphila.
33499919	12	124	theme	Lactobacillus	1785:1797	arg1	members					1774:1780	members	1774:1780	members of Lactobacillus	1774:1797	We found 24 microbial taxa associations with leptin deficiency, notably enrichment of members of Lactobacillus and depletion of Akkermansia muciniphila.
33499919	8	125	theme	body	1379:1382	arg1	mass					1384:1387	lean body mass	1374:1387	lean body mass	1374:1387	Pair-fed ob/ob mice exhibited even more compromised glucose metabolism and maintained strikingly similar high body fat percentage at the cost of lean body mass.
33499919	3	126	from	changes	409:415	arg1	microbiota					430:439	their gut microbiota	420:439	their gut microbiota	420:439	Specific changes in their gut microbiota such as decreased microbial diversity and increased Firmicutes to Bacteroidetes ratio have been suggested to contribute to obesity via increased microbiota capacity to harvest energy.
33499919	8	127	theme	Pair-fed	1229:1236	arg1	mice					1244:1247	Pair-fed ob/ob mice	1229:1247	Pair-fed ob/ob mice	1229:1247	Pair-fed ob/ob mice exhibited even more compromised glucose metabolism and maintained strikingly similar high body fat percentage at the cost of lean body mass.
33499919	7	128	theme	body	1208:1211	arg1	percentage					1217:1226	increased body fat percentage	1198:1226	increased body fat percentage	1198:1226	Compared to control mice, ob/ob mice fed ad libitum exhibited compromised glucose metabolism and increased body fat percentage.
33499919	3	129	theme	gut	426:428	arg1	microbiota					430:439	their gut microbiota	420:439	their gut microbiota	420:439	Specific changes in their gut microbiota such as decreased microbial diversity and increased Firmicutes to Bacteroidetes ratio have been suggested to contribute to obesity via increased microbiota capacity to harvest energy.
33499919	11	130	theme	community	1609:1617	arg1	different					1677:1685	different	1677:1685	different	1677:1685	Pair-feeding also altered several taxa, although the overall community composition at the end of the study was not significantly different.
33499919	11	130	theme	community	1609:1617	arg1	composition					1619:1629	the overall community composition	1597:1629	the overall community composition at the end of the study	1597:1653	Pair-feeding also altered several taxa, although the overall community composition at the end of the study was not significantly different.
33499919	11	131	theme	several	1574:1580	arg1	taxa					1582:1585	several taxa	1574:1585	several taxa	1574:1585	Pair-feeding also altered several taxa, although the overall community composition at the end of the study was not significantly different.
31417546	6	0	theme	short	871:875	arg1	acids					889:893	short chain fatty acids	871:893	short chain fatty acids	871:893	Concentration of short chain fatty acids were determined by GC-MS.
31417546	10	1	theme	microbiota	1454:1463	arg1	analysis					1465:1472	Further microbiota analysis	1446:1472	Further microbiota analysis	1446:1472	Further microbiota analysis revealed that LMP supplementation prevented T1D-associated caecal dysbiosis and selectively enriched caecal bacterial species to produce more SCFAs.
31417546	11	2	theme	Treg	1854:1857	arg1	population					1859:1868	increased Foxp3+ Treg population	1837:1868	increased Foxp3+ Treg population	1837:1868	The LMP-mediated microbial balance further enhanced caecal barrier function and shaped gut-pancreatic immune environment, as characterized by higher expression of tight junction proteins claudin-1, ZO-2 in caecum, increased Foxp3+ Treg population and decreased NLRP3 inflammasome activation in both caecum and pancreas.
31417546	11	2	theme	Treg	1854:1857	arg1	proteins					1801:1808	tight junction proteins claudin-1, ZO-2 in caecum, increased Foxp3+ Treg population and decreased NLRP3 inflammasome activation	1786:1912	tight junction proteins claudin-1, ZO-2 in caecum, increased Foxp3+ Treg population and decreased NLRP3 inflammasome activation	1786:1912	The LMP-mediated microbial balance further enhanced caecal barrier function and shaped gut-pancreatic immune environment, as characterized by higher expression of tight junction proteins claudin-1, ZO-2 in caecum, increased Foxp3+ Treg population and decreased NLRP3 inflammasome activation in both caecum and pancreas.
31417546	3	3	theme	%	479:479	arg1	LMP					489:491	5% (wt/wt) LMP	478:491	5% (wt/wt) LMP	478:491	Female NOD mice were weaned onto control or 5% (wt/wt) LMP supplemented diets for up to 40 weeks of age, overt diabetes incidence and blood glucose were monitored.
31417546	14	4	from	manipulation	2371:2382	arg1	humans					2409:2414	humans	2409:2414	humans	2409:2414	This finding opens a realistic option for gut microbiota manipulation and prevention of T1D in humans.
31417546	11	5	theme	LMP-mediated	1627:1638	arg1	balance					1650:1656	The LMP-mediated microbial balance	1623:1656	The LMP-mediated microbial balance	1623:1656	The LMP-mediated microbial balance further enhanced caecal barrier function and shaped gut-pancreatic immune environment, as characterized by higher expression of tight junction proteins claudin-1, ZO-2 in caecum, increased Foxp3+ Treg population and decreased NLRP3 inflammasome activation in both caecum and pancreas.
31417546	7	6	theme	inflammasome	1046:1057	arg1	activation					1059:1068	receptor protein 3 (NLRP3) inflammasome activation	1019:1068	receptor protein 3 (NLRP3) inflammasome activation	1019:1068	The barrier reinforcing tight junction proteins zonula occludens-2 (ZO-2), claudin-1 and NOD like receptor protein 3 (NLRP3) inflammasome activation were determined by Western blot.
31417546	5	7	used	used	794:797	arg2	sequencing					779:788	Next-generation sequencing	763:788	Next-generation sequencing	763:788	Next-generation sequencing was used for analyzing the composition of microbiota in caecum.
31417546	11	8	from	expression	1772:1781	arg1	pancreas					1933:1940	pancreas	1933:1940	pancreas	1933:1940	The LMP-mediated microbial balance further enhanced caecal barrier function and shaped gut-pancreatic immune environment, as characterized by higher expression of tight junction proteins claudin-1, ZO-2 in caecum, increased Foxp3+ Treg population and decreased NLRP3 inflammasome activation in both caecum and pancreas.
31417546	11	8	from	expression	1772:1781	arg1	caecum					1922:1927	caecum	1922:1927	caecum	1922:1927	The LMP-mediated microbial balance further enhanced caecal barrier function and shaped gut-pancreatic immune environment, as characterized by higher expression of tight junction proteins claudin-1, ZO-2 in caecum, increased Foxp3+ Treg population and decreased NLRP3 inflammasome activation in both caecum and pancreas.
31417546	14	9	from	prevention	2388:2397	arg1	humans					2409:2414	humans	2409:2414	humans	2409:2414	This finding opens a realistic option for gut microbiota manipulation and prevention of T1D in humans.
31417546	11	10	theme	decreased	1874:1882	arg1	activation					1903:1912	decreased NLRP3 inflammasome activation	1874:1912	decreased NLRP3 inflammasome activation	1874:1912	The LMP-mediated microbial balance further enhanced caecal barrier function and shaped gut-pancreatic immune environment, as characterized by higher expression of tight junction proteins claudin-1, ZO-2 in caecum, increased Foxp3+ Treg population and decreased NLRP3 inflammasome activation in both caecum and pancreas.
31417546	11	10	theme	decreased	1874:1882	arg1	proteins					1801:1808	tight junction proteins claudin-1, ZO-2 in caecum, increased Foxp3+ Treg population and decreased NLRP3 inflammasome activation	1786:1912	tight junction proteins claudin-1, ZO-2 in caecum, increased Foxp3+ Treg population and decreased NLRP3 inflammasome activation	1786:1912	The LMP-mediated microbial balance further enhanced caecal barrier function and shaped gut-pancreatic immune environment, as characterized by higher expression of tight junction proteins claudin-1, ZO-2 in caecum, increased Foxp3+ Treg population and decreased NLRP3 inflammasome activation in both caecum and pancreas.
31417546	10	11	theme	LMP	1488:1490	arg1	supplementation					1492:1506	LMP supplementation	1488:1506	LMP supplementation	1488:1506	Further microbiota analysis revealed that LMP supplementation prevented T1D-associated caecal dysbiosis and selectively enriched caecal bacterial species to produce more SCFAs.
31417546	7	12	theme	NLRP3	1039:1043	arg1	activation					1059:1068	receptor protein 3 (NLRP3) inflammasome activation	1019:1068	receptor protein 3 (NLRP3) inflammasome activation	1019:1068	The barrier reinforcing tight junction proteins zonula occludens-2 (ZO-2), claudin-1 and NOD like receptor protein 3 (NLRP3) inflammasome activation were determined by Western blot.
31417546	14	13	theme	gut	2356:2358	arg1	manipulation					2371:2382	gut microbiota manipulation	2356:2382	gut microbiota manipulation	2356:2382	This finding opens a realistic option for gut microbiota manipulation and prevention of T1D in humans.
31417546	1	14	theme	diabetes	166:173	arg1	development					144:154	the development	140:154	the development of type 1 diabetes (T1D)	140:179	Intestinal homeostasis underpins the development of type 1 diabetes (T1D), and dietary manipulations to enhance intestinal homeostasis have been proposed to prevent T1D.
31417546	8	15	theme	regulatory	1137:1146	arg1	cell					1150:1153	CD25+Foxp3+CD4+ regulatory T cell	1121:1153	CD25+Foxp3+CD4+ regulatory T cell (Foxp3+ Treg) in the pancreas	1121:1183	The proportion of CD25+Foxp3+CD4+ regulatory T cell (Foxp3+ Treg) in the pancreas, pancreatic and mesenteric lymph nodes was analyzed by flow cytometry.
31417546	8	15	theme	regulatory	1137:1146	arg1	Treg					1163:1166	Foxp3+ Treg	1156:1166	Foxp3+ Treg	1156:1166	The proportion of CD25+Foxp3+CD4+ regulatory T cell (Foxp3+ Treg) in the pancreas, pancreatic and mesenteric lymph nodes was analyzed by flow cytometry.
31417546	4	16	theme	broad-spectrum	603:616	arg1	ABX					631:633	ABX	631:633	ABX	631:633	Then broad-spectrum antibiotics (ABX) treatment per os for 7 days followed by gut microbiota transfer was performed to demonstrate gut microbiota-dependent effects.
31417546	4	16	theme	broad-spectrum	603:616	arg1	antibiotics					618:628	Then broad-spectrum antibiotics	598:628	Then broad-spectrum antibiotics (ABX) treatment per os for 7 days followed by gut microbiota transfer	598:698	Then broad-spectrum antibiotics (ABX) treatment per os for 7 days followed by gut microbiota transfer was performed to demonstrate gut microbiota-dependent effects.
31417546	11	17	theme	tight	1786:1790	arg1	claudin-1					1810:1818	claudin-1	1810:1818	claudin-1	1810:1818	The LMP-mediated microbial balance further enhanced caecal barrier function and shaped gut-pancreatic immune environment, as characterized by higher expression of tight junction proteins claudin-1, ZO-2 in caecum, increased Foxp3+ Treg population and decreased NLRP3 inflammasome activation in both caecum and pancreas.
31417546	11	17	theme	tight	1786:1790	arg1	activation					1903:1912	decreased NLRP3 inflammasome activation	1874:1912	decreased NLRP3 inflammasome activation	1874:1912	The LMP-mediated microbial balance further enhanced caecal barrier function and shaped gut-pancreatic immune environment, as characterized by higher expression of tight junction proteins claudin-1, ZO-2 in caecum, increased Foxp3+ Treg population and decreased NLRP3 inflammasome activation in both caecum and pancreas.
31417546	11	17	theme	tight	1786:1790	arg1	population					1859:1868	increased Foxp3+ Treg population	1837:1868	increased Foxp3+ Treg population	1837:1868	The LMP-mediated microbial balance further enhanced caecal barrier function and shaped gut-pancreatic immune environment, as characterized by higher expression of tight junction proteins claudin-1, ZO-2 in caecum, increased Foxp3+ Treg population and decreased NLRP3 inflammasome activation in both caecum and pancreas.
31417546	11	17	theme	tight	1786:1790	arg1	proteins					1801:1808	tight junction proteins claudin-1, ZO-2 in caecum, increased Foxp3+ Treg population and decreased NLRP3 inflammasome activation	1786:1912	tight junction proteins claudin-1, ZO-2 in caecum, increased Foxp3+ Treg population and decreased NLRP3 inflammasome activation	1786:1912	The LMP-mediated microbial balance further enhanced caecal barrier function and shaped gut-pancreatic immune environment, as characterized by higher expression of tight junction proteins claudin-1, ZO-2 in caecum, increased Foxp3+ Treg population and decreased NLRP3 inflammasome activation in both caecum and pancreas.
31417546	11	17	theme	tight	1786:1790	arg1	ZO-2					1821:1824	ZO-2	1821:1824	ZO-2 in caecum	1821:1834	The LMP-mediated microbial balance further enhanced caecal barrier function and shaped gut-pancreatic immune environment, as characterized by higher expression of tight junction proteins claudin-1, ZO-2 in caecum, increased Foxp3+ Treg population and decreased NLRP3 inflammasome activation in both caecum and pancreas.
31417546	9	18	theme	diabetes	1379:1386	arg1	incidence					1388:1396	diabetes incidence	1379:1396	diabetes incidence	1379:1396	We found that LMP supplementation ameliorated T1D development in non-obese diabetic (NOD) mice, as evidenced by decreasing diabetes incidence and fasting glucose levels in LMP fed NOD mice.
31417546	8	19	theme	cell	1150:1153	arg1	proportion					1107:1116	The proportion	1103:1116	The proportion of CD25+Foxp3+CD4+ regulatory T cell (Foxp3+ Treg) in the pancreas, pancreatic and mesenteric lymph nodes	1103:1222	The proportion of CD25+Foxp3+CD4+ regulatory T cell (Foxp3+ Treg) in the pancreas, pancreatic and mesenteric lymph nodes was analyzed by flow cytometry.
31417546	12	20	theme	T1D	2094:2096	arg1	autoimmunity					2098:2109	T1D autoimmunity	2094:2109	T1D autoimmunity	2094:2109	The microbiota-dependent beneficial effect of LMP on T1D was further proven by the fact that aberration of caecal microbiota by ABX treatment worsened T1D autoimmunity and could be restored with transfer of feces of LMP-fed NOD mice.
31417546	10	21	theme	caecal	1533:1538	arg1	dysbiosis					1540:1548	T1D-associated caecal dysbiosis	1518:1548	T1D-associated caecal dysbiosis	1518:1548	Further microbiota analysis revealed that LMP supplementation prevented T1D-associated caecal dysbiosis and selectively enriched caecal bacterial species to produce more SCFAs.
31417546	7	22	theme	receptor	1019:1026	arg1	activation					1059:1068	receptor protein 3 (NLRP3) inflammasome activation	1019:1068	receptor protein 3 (NLRP3) inflammasome activation	1019:1068	The barrier reinforcing tight junction proteins zonula occludens-2 (ZO-2), claudin-1 and NOD like receptor protein 3 (NLRP3) inflammasome activation were determined by Western blot.
31417546	13	23	theme	immune	2294:2299	arg1	environment					2301:2311	shape pancreatic immune environment	2277:2311	shape pancreatic immune environment	2277:2311	These data demonstrate that this novel LMP limits T1D development by inducing caecal homeostasis to shape pancreatic immune environment.
31417546	8	24	theme	Foxp3+	1156:1161	arg1	cell					1150:1153	CD25+Foxp3+CD4+ regulatory T cell	1121:1153	CD25+Foxp3+CD4+ regulatory T cell (Foxp3+ Treg) in the pancreas	1121:1183	The proportion of CD25+Foxp3+CD4+ regulatory T cell (Foxp3+ Treg) in the pancreas, pancreatic and mesenteric lymph nodes was analyzed by flow cytometry.
31417546	8	24	theme	Foxp3+	1156:1161	arg1	Treg					1163:1166	Foxp3+ Treg	1156:1166	Foxp3+ Treg	1156:1166	The proportion of CD25+Foxp3+CD4+ regulatory T cell (Foxp3+ Treg) in the pancreas, pancreatic and mesenteric lymph nodes was analyzed by flow cytometry.
31417546	12	25	theme	microbiota	2057:2066	arg1	aberration					2036:2045	aberration	2036:2045	aberration of caecal microbiota by ABX treatment	2036:2083	The microbiota-dependent beneficial effect of LMP on T1D was further proven by the fact that aberration of caecal microbiota by ABX treatment worsened T1D autoimmunity and could be restored with transfer of feces of LMP-fed NOD mice.
31417546	11	26	theme	NLRP3	1884:1888	arg1	activation					1903:1912	decreased NLRP3 inflammasome activation	1874:1912	decreased NLRP3 inflammasome activation	1874:1912	The LMP-mediated microbial balance further enhanced caecal barrier function and shaped gut-pancreatic immune environment, as characterized by higher expression of tight junction proteins claudin-1, ZO-2 in caecum, increased Foxp3+ Treg population and decreased NLRP3 inflammasome activation in both caecum and pancreas.
31417546	11	26	theme	NLRP3	1884:1888	arg1	proteins					1801:1808	tight junction proteins claudin-1, ZO-2 in caecum, increased Foxp3+ Treg population and decreased NLRP3 inflammasome activation	1786:1912	tight junction proteins claudin-1, ZO-2 in caecum, increased Foxp3+ Treg population and decreased NLRP3 inflammasome activation	1786:1912	The LMP-mediated microbial balance further enhanced caecal barrier function and shaped gut-pancreatic immune environment, as characterized by higher expression of tight junction proteins claudin-1, ZO-2 in caecum, increased Foxp3+ Treg population and decreased NLRP3 inflammasome activation in both caecum and pancreas.
31417546	8	27	theme	lymph	1212:1216	arg1	nodes					1218:1222	mesenteric lymph nodes	1201:1222	mesenteric lymph nodes	1201:1222	The proportion of CD25+Foxp3+CD4+ regulatory T cell (Foxp3+ Treg) in the pancreas, pancreatic and mesenteric lymph nodes was analyzed by flow cytometry.
31417546	10	28	theme	bacterial	1582:1590	arg1	species					1592:1598	selectively enriched caecal bacterial species	1554:1598	selectively enriched caecal bacterial species	1554:1598	Further microbiota analysis revealed that LMP supplementation prevented T1D-associated caecal dysbiosis and selectively enriched caecal bacterial species to produce more SCFAs.
31417546	3	29	theme	age	534:536	arg1	weeks					525:529	up to 40 weeks	516:529	up to 40 weeks of age	516:536	Female NOD mice were weaned onto control or 5% (wt/wt) LMP supplemented diets for up to 40 weeks of age, overt diabetes incidence and blood glucose were monitored.
31417546	9	30	theme	glucose	1410:1416	arg1	levels					1418:1423	fasting glucose levels	1402:1423	fasting glucose levels	1402:1423	We found that LMP supplementation ameliorated T1D development in non-obese diabetic (NOD) mice, as evidenced by decreasing diabetes incidence and fasting glucose levels in LMP fed NOD mice.
31417546	12	31	theme	microbiota-dependent	1947:1966	arg1	effect					1979:1984	The microbiota-dependent beneficial effect	1943:1984	The microbiota-dependent beneficial effect of LMP on T1D	1943:1998	The microbiota-dependent beneficial effect of LMP on T1D was further proven by the fact that aberration of caecal microbiota by ABX treatment worsened T1D autoimmunity and could be restored with transfer of feces of LMP-fed NOD mice.
31417546	10	32	theme	enriched	1566:1573	arg1	species					1592:1598	selectively enriched caecal bacterial species	1554:1598	selectively enriched caecal bacterial species	1554:1598	Further microbiota analysis revealed that LMP supplementation prevented T1D-associated caecal dysbiosis and selectively enriched caecal bacterial species to produce more SCFAs.
31417546	11	33	theme	proteins	1801:1808	arg1	expression					1772:1781	higher expression	1765:1781	higher expression of tight junction proteins claudin-1, ZO-2 in caecum, increased Foxp3+ Treg population and decreased NLRP3 inflammasome activation in both caecum and pancreas	1765:1940	The LMP-mediated microbial balance further enhanced caecal barrier function and shaped gut-pancreatic immune environment, as characterized by higher expression of tight junction proteins claudin-1, ZO-2 in caecum, increased Foxp3+ Treg population and decreased NLRP3 inflammasome activation in both caecum and pancreas.
31417546	1	34	theme	Intestinal	107:116	arg1	homeostasis					118:128	Intestinal homeostasis	107:128	Intestinal homeostasis	107:128	Intestinal homeostasis underpins the development of type 1 diabetes (T1D), and dietary manipulations to enhance intestinal homeostasis have been proposed to prevent T1D.
31417546	6	35	theme	fatty	883:887	arg1	acids					889:893	short chain fatty acids	871:893	short chain fatty acids	871:893	Concentration of short chain fatty acids were determined by GC-MS.
31417546	13	36	theme	T1D	2227:2229	arg1	development					2231:2241	T1D development	2227:2241	T1D development	2227:2241	These data demonstrate that this novel LMP limits T1D development by inducing caecal homeostasis to shape pancreatic immune environment.
31417546	7	37	theme	tight	945:949	arg1	NOD					1010:1012	NOD	1010:1012	NOD	1010:1012	The barrier reinforcing tight junction proteins zonula occludens-2 (ZO-2), claudin-1 and NOD like receptor protein 3 (NLRP3) inflammasome activation were determined by Western blot.
31417546	7	37	theme	tight	945:949	arg1	claudin-1					996:1004	claudin-1	996:1004	claudin-1	996:1004	The barrier reinforcing tight junction proteins zonula occludens-2 (ZO-2), claudin-1 and NOD like receptor protein 3 (NLRP3) inflammasome activation were determined by Western blot.
31417546	7	37	theme	tight	945:949	arg1	occludens-2					976:986	zonula occludens-2	969:986	zonula occludens-2 (ZO-2)	969:993	The barrier reinforcing tight junction proteins zonula occludens-2 (ZO-2), claudin-1 and NOD like receptor protein 3 (NLRP3) inflammasome activation were determined by Western blot.
31417546	7	37	theme	tight	945:949	arg1	proteins					960:967	The barrier reinforcing tight junction proteins	921:967	The barrier reinforcing tight junction proteins zonula occludens-2 (ZO-2), claudin-1 and NOD like receptor protein 3 (NLRP3) inflammasome activation	921:1068	The barrier reinforcing tight junction proteins zonula occludens-2 (ZO-2), claudin-1 and NOD like receptor protein 3 (NLRP3) inflammasome activation were determined by Western blot.
31417546	9	38	theme	fed	1432:1434	arg1	mice					1440:1443	LMP fed NOD mice	1428:1443	LMP fed NOD mice	1428:1443	We found that LMP supplementation ameliorated T1D development in non-obese diabetic (NOD) mice, as evidenced by decreasing diabetes incidence and fasting glucose levels in LMP fed NOD mice.
31417546	11	39	theme	gut-pancreatic	1710:1723	arg1	environment					1732:1742	shaped gut-pancreatic immune environment	1703:1742	shaped gut-pancreatic immune environment	1703:1742	The LMP-mediated microbial balance further enhanced caecal barrier function and shaped gut-pancreatic immune environment, as characterized by higher expression of tight junction proteins claudin-1, ZO-2 in caecum, increased Foxp3+ Treg population and decreased NLRP3 inflammasome activation in both caecum and pancreas.
31417546	5	40	theme	Next-generation	763:777	arg1	sequencing					779:788	Next-generation sequencing	763:788	Next-generation sequencing	763:788	Next-generation sequencing was used for analyzing the composition of microbiota in caecum.
31417546	7	41	theme	barrier	925:931	arg1	NOD					1010:1012	NOD	1010:1012	NOD	1010:1012	The barrier reinforcing tight junction proteins zonula occludens-2 (ZO-2), claudin-1 and NOD like receptor protein 3 (NLRP3) inflammasome activation were determined by Western blot.
31417546	7	41	theme	barrier	925:931	arg1	claudin-1					996:1004	claudin-1	996:1004	claudin-1	996:1004	The barrier reinforcing tight junction proteins zonula occludens-2 (ZO-2), claudin-1 and NOD like receptor protein 3 (NLRP3) inflammasome activation were determined by Western blot.
31417546	7	41	theme	barrier	925:931	arg1	occludens-2					976:986	zonula occludens-2	969:986	zonula occludens-2 (ZO-2)	969:993	The barrier reinforcing tight junction proteins zonula occludens-2 (ZO-2), claudin-1 and NOD like receptor protein 3 (NLRP3) inflammasome activation were determined by Western blot.
31417546	7	41	theme	barrier	925:931	arg1	proteins					960:967	The barrier reinforcing tight junction proteins	921:967	The barrier reinforcing tight junction proteins zonula occludens-2 (ZO-2), claudin-1 and NOD like receptor protein 3 (NLRP3) inflammasome activation	921:1068	The barrier reinforcing tight junction proteins zonula occludens-2 (ZO-2), claudin-1 and NOD like receptor protein 3 (NLRP3) inflammasome activation were determined by Western blot.
31417546	11	42	from	activation	1903:1912	arg1	caecum					1829:1834	caecum	1829:1834	caecum	1829:1834	The LMP-mediated microbial balance further enhanced caecal barrier function and shaped gut-pancreatic immune environment, as characterized by higher expression of tight junction proteins claudin-1, ZO-2 in caecum, increased Foxp3+ Treg population and decreased NLRP3 inflammasome activation in both caecum and pancreas.
31417546	12	43	theme	caecal	2050:2055	arg1	microbiota					2057:2066	caecal microbiota	2050:2066	caecal microbiota	2050:2066	The microbiota-dependent beneficial effect of LMP on T1D was further proven by the fact that aberration of caecal microbiota by ABX treatment worsened T1D autoimmunity and could be restored with transfer of feces of LMP-fed NOD mice.
31417546	1	44	theme	type	159:162	arg1	T1D					176:178	T1D	176:178	T1D	176:178	Intestinal homeostasis underpins the development of type 1 diabetes (T1D), and dietary manipulations to enhance intestinal homeostasis have been proposed to prevent T1D.
31417546	1	44	theme	type	159:162	arg1	diabetes					166:173	type 1 diabetes	159:173	type 1 diabetes (T1D)	159:179	Intestinal homeostasis underpins the development of type 1 diabetes (T1D), and dietary manipulations to enhance intestinal homeostasis have been proposed to prevent T1D.
31417546	4	45	theme	microbiota	680:689	arg1	transfer					691:698	gut microbiota transfer	676:698	gut microbiota transfer	676:698	Then broad-spectrum antibiotics (ABX) treatment per os for 7 days followed by gut microbiota transfer was performed to demonstrate gut microbiota-dependent effects.
31417546	5	46	from	composition	817:827	arg1	caecum					846:851	caecum	846:851	caecum	846:851	Next-generation sequencing was used for analyzing the composition of microbiota in caecum.
31417546	11	47	dep	proteins	1801:1808	arg1	claudin-1					1810:1818	claudin-1	1810:1818	claudin-1	1810:1818	The LMP-mediated microbial balance further enhanced caecal barrier function and shaped gut-pancreatic immune environment, as characterized by higher expression of tight junction proteins claudin-1, ZO-2 in caecum, increased Foxp3+ Treg population and decreased NLRP3 inflammasome activation in both caecum and pancreas.
31417546	11	47	dep	proteins	1801:1808	arg1	activation					1903:1912	decreased NLRP3 inflammasome activation	1874:1912	decreased NLRP3 inflammasome activation	1874:1912	The LMP-mediated microbial balance further enhanced caecal barrier function and shaped gut-pancreatic immune environment, as characterized by higher expression of tight junction proteins claudin-1, ZO-2 in caecum, increased Foxp3+ Treg population and decreased NLRP3 inflammasome activation in both caecum and pancreas.
31417546	11	47	dep	proteins	1801:1808	arg1	population					1859:1868	increased Foxp3+ Treg population	1837:1868	increased Foxp3+ Treg population	1837:1868	The LMP-mediated microbial balance further enhanced caecal barrier function and shaped gut-pancreatic immune environment, as characterized by higher expression of tight junction proteins claudin-1, ZO-2 in caecum, increased Foxp3+ Treg population and decreased NLRP3 inflammasome activation in both caecum and pancreas.
31417546	11	47	dep	proteins	1801:1808	arg1	proteins					1801:1808	tight junction proteins claudin-1, ZO-2 in caecum, increased Foxp3+ Treg population and decreased NLRP3 inflammasome activation	1786:1912	tight junction proteins claudin-1, ZO-2 in caecum, increased Foxp3+ Treg population and decreased NLRP3 inflammasome activation	1786:1912	The LMP-mediated microbial balance further enhanced caecal barrier function and shaped gut-pancreatic immune environment, as characterized by higher expression of tight junction proteins claudin-1, ZO-2 in caecum, increased Foxp3+ Treg population and decreased NLRP3 inflammasome activation in both caecum and pancreas.
31417546	11	47	dep	proteins	1801:1808	arg1	ZO-2					1821:1824	ZO-2	1821:1824	ZO-2 in caecum	1821:1834	The LMP-mediated microbial balance further enhanced caecal barrier function and shaped gut-pancreatic immune environment, as characterized by higher expression of tight junction proteins claudin-1, ZO-2 in caecum, increased Foxp3+ Treg population and decreased NLRP3 inflammasome activation in both caecum and pancreas.
31417546	3	48	theme	supplemented	493:504	arg1	diets					506:510	supplemented diets	493:510	supplemented diets	493:510	Female NOD mice were weaned onto control or 5% (wt/wt) LMP supplemented diets for up to 40 weeks of age, overt diabetes incidence and blood glucose were monitored.
31417546	13	49	theme	shape	2277:2281	arg1	environment					2301:2311	shape pancreatic immune environment	2277:2311	shape pancreatic immune environment	2277:2311	These data demonstrate that this novel LMP limits T1D development by inducing caecal homeostasis to shape pancreatic immune environment.
31417546	11	50	theme	inflammasome	1890:1901	arg1	activation					1903:1912	decreased NLRP3 inflammasome activation	1874:1912	decreased NLRP3 inflammasome activation	1874:1912	The LMP-mediated microbial balance further enhanced caecal barrier function and shaped gut-pancreatic immune environment, as characterized by higher expression of tight junction proteins claudin-1, ZO-2 in caecum, increased Foxp3+ Treg population and decreased NLRP3 inflammasome activation in both caecum and pancreas.
31417546	11	50	theme	inflammasome	1890:1901	arg1	proteins					1801:1808	tight junction proteins claudin-1, ZO-2 in caecum, increased Foxp3+ Treg population and decreased NLRP3 inflammasome activation	1786:1912	tight junction proteins claudin-1, ZO-2 in caecum, increased Foxp3+ Treg population and decreased NLRP3 inflammasome activation	1786:1912	The LMP-mediated microbial balance further enhanced caecal barrier function and shaped gut-pancreatic immune environment, as characterized by higher expression of tight junction proteins claudin-1, ZO-2 in caecum, increased Foxp3+ Treg population and decreased NLRP3 inflammasome activation in both caecum and pancreas.
31417546	0	51	theme	Diabetic	92:99	arg1	Mice					101:104	Non-obese Diabetic Mice	82:104	Non-obese Diabetic Mice	82:104	Modulation of Gut Microbiota by Low Methoxyl Pectin Attenuates Type 1 Diabetes in Non-obese Diabetic Mice.
31417546	0	52	theme	Low	32:34	arg1	Pectin					45:50	Low Methoxyl Pectin	32:50	Low Methoxyl Pectin	32:50	Modulation of Gut Microbiota by Low Methoxyl Pectin Attenuates Type 1 Diabetes in Non-obese Diabetic Mice.
31417546	3	53	dep	control	467:473	arg1	diets					506:510	supplemented diets	493:510	supplemented diets	493:510	Female NOD mice were weaned onto control or 5% (wt/wt) LMP supplemented diets for up to 40 weeks of age, overt diabetes incidence and blood glucose were monitored.
31417546	2	54	theme	low-methoxyl	363:374	arg1	pectin					376:381	specific low-methoxyl pectin	354:381	a novel specific low-methoxyl pectin (LMP) dietary fiber	346:401	The current study aimed to investigate the efficacy of supplementing a novel specific low-methoxyl pectin (LMP) dietary fiber in preventing T1D development.
31417546	2	54	theme	low-methoxyl	363:374	arg1	LMP					384:386	LMP	384:386	LMP	384:386	The current study aimed to investigate the efficacy of supplementing a novel specific low-methoxyl pectin (LMP) dietary fiber in preventing T1D development.
31417546	3	55	theme	NOD	441:443	arg1	mice					445:448	Female NOD mice	434:448	Female NOD mice	434:448	Female NOD mice were weaned onto control or 5% (wt/wt) LMP supplemented diets for up to 40 weeks of age, overt diabetes incidence and blood glucose were monitored.
31417546	0	56	theme	Gut	14:16	arg1	Microbiota					18:27	Gut Microbiota	14:27	Gut Microbiota	14:27	Modulation of Gut Microbiota by Low Methoxyl Pectin Attenuates Type 1 Diabetes in Non-obese Diabetic Mice.
31417546	9	57	theme	LMP	1270:1272	arg1	supplementation					1274:1288	LMP supplementation	1270:1288	LMP supplementation	1270:1288	We found that LMP supplementation ameliorated T1D development in non-obese diabetic (NOD) mice, as evidenced by decreasing diabetes incidence and fasting glucose levels in LMP fed NOD mice.
31417546	5	58	theme	microbiota	832:841	arg1	composition					817:827	the composition	813:827	the composition of microbiota in caecum	813:851	Next-generation sequencing was used for analyzing the composition of microbiota in caecum.
31417546	11	59	theme	caecal	1675:1680	arg1	function					1690:1697	caecal barrier function	1675:1697	caecal barrier function	1675:1697	The LMP-mediated microbial balance further enhanced caecal barrier function and shaped gut-pancreatic immune environment, as characterized by higher expression of tight junction proteins claudin-1, ZO-2 in caecum, increased Foxp3+ Treg population and decreased NLRP3 inflammasome activation in both caecum and pancreas.
31417546	12	60	theme	NOD	2167:2169	arg1	mice					2171:2174	LMP-fed NOD mice	2159:2174	LMP-fed NOD mice	2159:2174	The microbiota-dependent beneficial effect of LMP on T1D was further proven by the fact that aberration of caecal microbiota by ABX treatment worsened T1D autoimmunity and could be restored with transfer of feces of LMP-fed NOD mice.
31417546	12	61	from	effect	1979:1984	arg1	T1D					1996:1998	T1D	1996:1998	T1D	1996:1998	The microbiota-dependent beneficial effect of LMP on T1D was further proven by the fact that aberration of caecal microbiota by ABX treatment worsened T1D autoimmunity and could be restored with transfer of feces of LMP-fed NOD mice.
31417546	10	62	theme	Further	1446:1452	arg1	analysis					1465:1472	Further microbiota analysis	1446:1472	Further microbiota analysis	1446:1472	Further microbiota analysis revealed that LMP supplementation prevented T1D-associated caecal dysbiosis and selectively enriched caecal bacterial species to produce more SCFAs.
31417546	3	63	theme	blood	568:572	arg1	glucose					574:580	blood glucose	568:580	blood glucose	568:580	Female NOD mice were weaned onto control or 5% (wt/wt) LMP supplemented diets for up to 40 weeks of age, overt diabetes incidence and blood glucose were monitored.
31417546	0	64	theme	Methoxyl	36:43	arg1	Pectin					45:50	Low Methoxyl Pectin	32:50	Low Methoxyl Pectin	32:50	Modulation of Gut Microbiota by Low Methoxyl Pectin Attenuates Type 1 Diabetes in Non-obese Diabetic Mice.
31417546	3	65	theme	overt	539:543	arg1	incidence					554:562	overt diabetes incidence	539:562	overt diabetes incidence	539:562	Female NOD mice were weaned onto control or 5% (wt/wt) LMP supplemented diets for up to 40 weeks of age, overt diabetes incidence and blood glucose were monitored.
31417546	11	66	theme	shaped	1703:1708	arg1	environment					1732:1742	shaped gut-pancreatic immune environment	1703:1742	shaped gut-pancreatic immune environment	1703:1742	The LMP-mediated microbial balance further enhanced caecal barrier function and shaped gut-pancreatic immune environment, as characterized by higher expression of tight junction proteins claudin-1, ZO-2 in caecum, increased Foxp3+ Treg population and decreased NLRP3 inflammasome activation in both caecum and pancreas.
31417546	12	67	theme	LMP-fed	2159:2165	arg1	mice					2171:2174	LMP-fed NOD mice	2159:2174	LMP-fed NOD mice	2159:2174	The microbiota-dependent beneficial effect of LMP on T1D was further proven by the fact that aberration of caecal microbiota by ABX treatment worsened T1D autoimmunity and could be restored with transfer of feces of LMP-fed NOD mice.
31417546	9	68	theme	non-obese	1321:1329	arg1	mice					1346:1349	non-obese diabetic (NOD) mice	1321:1349	non-obese diabetic (NOD) mice	1321:1349	We found that LMP supplementation ameliorated T1D development in non-obese diabetic (NOD) mice, as evidenced by decreasing diabetes incidence and fasting glucose levels in LMP fed NOD mice.
31417546	4	69	theme	antibiotics	618:628	arg1	treatment					636:644	Then broad-spectrum antibiotics (ABX) treatment	598:644	Then broad-spectrum antibiotics (ABX) treatment per os for 7 days followed by gut microbiota transfer	598:698	Then broad-spectrum antibiotics (ABX) treatment per os for 7 days followed by gut microbiota transfer was performed to demonstrate gut microbiota-dependent effects.
31417546	4	70	theme	gut	729:731	arg1	effects					754:760	gut microbiota-dependent effects	729:760	gut microbiota-dependent effects	729:760	Then broad-spectrum antibiotics (ABX) treatment per os for 7 days followed by gut microbiota transfer was performed to demonstrate gut microbiota-dependent effects.
31417546	2	71	theme	novel	348:352	arg1	fiber					397:401	a novel specific low-methoxyl pectin (LMP) dietary fiber	346:401	a novel specific low-methoxyl pectin (LMP) dietary fiber	346:401	The current study aimed to investigate the efficacy of supplementing a novel specific low-methoxyl pectin (LMP) dietary fiber in preventing T1D development.
31417546	8	72	from	cell	1150:1153	arg1	pancreas					1176:1183	the pancreas	1172:1183	the pancreas	1172:1183	The proportion of CD25+Foxp3+CD4+ regulatory T cell (Foxp3+ Treg) in the pancreas, pancreatic and mesenteric lymph nodes was analyzed by flow cytometry.
31417546	11	73	theme	Foxp3+	1847:1852	arg1	population					1859:1868	increased Foxp3+ Treg population	1837:1868	increased Foxp3+ Treg population	1837:1868	The LMP-mediated microbial balance further enhanced caecal barrier function and shaped gut-pancreatic immune environment, as characterized by higher expression of tight junction proteins claudin-1, ZO-2 in caecum, increased Foxp3+ Treg population and decreased NLRP3 inflammasome activation in both caecum and pancreas.
31417546	11	73	theme	Foxp3+	1847:1852	arg1	proteins					1801:1808	tight junction proteins claudin-1, ZO-2 in caecum, increased Foxp3+ Treg population and decreased NLRP3 inflammasome activation	1786:1912	tight junction proteins claudin-1, ZO-2 in caecum, increased Foxp3+ Treg population and decreased NLRP3 inflammasome activation	1786:1912	The LMP-mediated microbial balance further enhanced caecal barrier function and shaped gut-pancreatic immune environment, as characterized by higher expression of tight junction proteins claudin-1, ZO-2 in caecum, increased Foxp3+ Treg population and decreased NLRP3 inflammasome activation in both caecum and pancreas.
31417546	11	74	from	claudin-1	1810:1818	arg1	caecum					1829:1834	caecum	1829:1834	caecum	1829:1834	The LMP-mediated microbial balance further enhanced caecal barrier function and shaped gut-pancreatic immune environment, as characterized by higher expression of tight junction proteins claudin-1, ZO-2 in caecum, increased Foxp3+ Treg population and decreased NLRP3 inflammasome activation in both caecum and pancreas.
31417546	10	75	theme	T1D-associated	1518:1531	arg1	dysbiosis					1540:1548	T1D-associated caecal dysbiosis	1518:1548	T1D-associated caecal dysbiosis	1518:1548	Further microbiota analysis revealed that LMP supplementation prevented T1D-associated caecal dysbiosis and selectively enriched caecal bacterial species to produce more SCFAs.
31417546	11	76	from	ZO-2	1821:1824	arg1	caecum					1829:1834	caecum	1829:1834	caecum	1829:1834	The LMP-mediated microbial balance further enhanced caecal barrier function and shaped gut-pancreatic immune environment, as characterized by higher expression of tight junction proteins claudin-1, ZO-2 in caecum, increased Foxp3+ Treg population and decreased NLRP3 inflammasome activation in both caecum and pancreas.
31417546	11	77	theme	microbial	1640:1648	arg1	balance					1650:1656	The LMP-mediated microbial balance	1623:1656	The LMP-mediated microbial balance	1623:1656	The LMP-mediated microbial balance further enhanced caecal barrier function and shaped gut-pancreatic immune environment, as characterized by higher expression of tight junction proteins claudin-1, ZO-2 in caecum, increased Foxp3+ Treg population and decreased NLRP3 inflammasome activation in both caecum and pancreas.
31417546	14	78	theme	microbiota	2360:2369	arg1	manipulation					2371:2382	gut microbiota manipulation	2356:2382	gut microbiota manipulation	2356:2382	This finding opens a realistic option for gut microbiota manipulation and prevention of T1D in humans.
31417546	3	79	dep	40	522:523	arg1	to					519:520	to	519:520	to	519:520	Female NOD mice were weaned onto control or 5% (wt/wt) LMP supplemented diets for up to 40 weeks of age, overt diabetes incidence and blood glucose were monitored.
31417546	12	80	theme	beneficial	1968:1977	arg1	effect					1979:1984	The microbiota-dependent beneficial effect	1943:1984	The microbiota-dependent beneficial effect of LMP on T1D	1943:1998	The microbiota-dependent beneficial effect of LMP on T1D was further proven by the fact that aberration of caecal microbiota by ABX treatment worsened T1D autoimmunity and could be restored with transfer of feces of LMP-fed NOD mice.
31417546	7	81	theme	protein	1028:1034	arg1	activation					1059:1068	receptor protein 3 (NLRP3) inflammasome activation	1019:1068	receptor protein 3 (NLRP3) inflammasome activation	1019:1068	The barrier reinforcing tight junction proteins zonula occludens-2 (ZO-2), claudin-1 and NOD like receptor protein 3 (NLRP3) inflammasome activation were determined by Western blot.
31417546	8	82	theme	CD25+Foxp3+CD4+	1121:1135	arg1	cell					1150:1153	CD25+Foxp3+CD4+ regulatory T cell	1121:1153	CD25+Foxp3+CD4+ regulatory T cell (Foxp3+ Treg) in the pancreas	1121:1183	The proportion of CD25+Foxp3+CD4+ regulatory T cell (Foxp3+ Treg) in the pancreas, pancreatic and mesenteric lymph nodes was analyzed by flow cytometry.
31417546	8	82	theme	CD25+Foxp3+CD4+	1121:1135	arg1	Treg					1163:1166	Foxp3+ Treg	1156:1166	Foxp3+ Treg	1156:1166	The proportion of CD25+Foxp3+CD4+ regulatory T cell (Foxp3+ Treg) in the pancreas, pancreatic and mesenteric lymph nodes was analyzed by flow cytometry.
31417546	11	83	theme	junction	1792:1799	arg1	claudin-1					1810:1818	claudin-1	1810:1818	claudin-1	1810:1818	The LMP-mediated microbial balance further enhanced caecal barrier function and shaped gut-pancreatic immune environment, as characterized by higher expression of tight junction proteins claudin-1, ZO-2 in caecum, increased Foxp3+ Treg population and decreased NLRP3 inflammasome activation in both caecum and pancreas.
31417546	11	83	theme	junction	1792:1799	arg1	activation					1903:1912	decreased NLRP3 inflammasome activation	1874:1912	decreased NLRP3 inflammasome activation	1874:1912	The LMP-mediated microbial balance further enhanced caecal barrier function and shaped gut-pancreatic immune environment, as characterized by higher expression of tight junction proteins claudin-1, ZO-2 in caecum, increased Foxp3+ Treg population and decreased NLRP3 inflammasome activation in both caecum and pancreas.
31417546	11	83	theme	junction	1792:1799	arg1	population					1859:1868	increased Foxp3+ Treg population	1837:1868	increased Foxp3+ Treg population	1837:1868	The LMP-mediated microbial balance further enhanced caecal barrier function and shaped gut-pancreatic immune environment, as characterized by higher expression of tight junction proteins claudin-1, ZO-2 in caecum, increased Foxp3+ Treg population and decreased NLRP3 inflammasome activation in both caecum and pancreas.
31417546	11	83	theme	junction	1792:1799	arg1	proteins					1801:1808	tight junction proteins claudin-1, ZO-2 in caecum, increased Foxp3+ Treg population and decreased NLRP3 inflammasome activation	1786:1912	tight junction proteins claudin-1, ZO-2 in caecum, increased Foxp3+ Treg population and decreased NLRP3 inflammasome activation	1786:1912	The LMP-mediated microbial balance further enhanced caecal barrier function and shaped gut-pancreatic immune environment, as characterized by higher expression of tight junction proteins claudin-1, ZO-2 in caecum, increased Foxp3+ Treg population and decreased NLRP3 inflammasome activation in both caecum and pancreas.
31417546	11	83	theme	junction	1792:1799	arg1	ZO-2					1821:1824	ZO-2	1821:1824	ZO-2 in caecum	1821:1834	The LMP-mediated microbial balance further enhanced caecal barrier function and shaped gut-pancreatic immune environment, as characterized by higher expression of tight junction proteins claudin-1, ZO-2 in caecum, increased Foxp3+ Treg population and decreased NLRP3 inflammasome activation in both caecum and pancreas.
31417546	14	84	theme	realistic	2335:2343	arg1	option					2345:2350	a realistic option	2333:2350	a realistic option for gut microbiota manipulation and prevention of T1D in humans	2333:2414	This finding opens a realistic option for gut microbiota manipulation and prevention of T1D in humans.
31417546	2	85	theme	current	281:287	arg1	study					289:293	The current study	277:293	The current study	277:293	The current study aimed to investigate the efficacy of supplementing a novel specific low-methoxyl pectin (LMP) dietary fiber in preventing T1D development.
31417546	13	86	theme	pancreatic	2283:2292	arg1	environment					2301:2311	shape pancreatic immune environment	2277:2311	shape pancreatic immune environment	2277:2311	These data demonstrate that this novel LMP limits T1D development by inducing caecal homeostasis to shape pancreatic immune environment.
31417546	8	87	theme	T	1148:1148	arg1	cell					1150:1153	CD25+Foxp3+CD4+ regulatory T cell	1121:1153	CD25+Foxp3+CD4+ regulatory T cell (Foxp3+ Treg) in the pancreas	1121:1183	The proportion of CD25+Foxp3+CD4+ regulatory T cell (Foxp3+ Treg) in the pancreas, pancreatic and mesenteric lymph nodes was analyzed by flow cytometry.
31417546	8	87	theme	T	1148:1148	arg1	Treg					1163:1166	Foxp3+ Treg	1156:1166	Foxp3+ Treg	1156:1166	The proportion of CD25+Foxp3+CD4+ regulatory T cell (Foxp3+ Treg) in the pancreas, pancreatic and mesenteric lymph nodes was analyzed by flow cytometry.
31417546	0	88	from	Diabetes	70:77	arg1	Mice					101:104	Non-obese Diabetic Mice	82:104	Non-obese Diabetic Mice	82:104	Modulation of Gut Microbiota by Low Methoxyl Pectin Attenuates Type 1 Diabetes in Non-obese Diabetic Mice.
31417546	8	89	theme	pancreatic	1186:1195	arg1	proportion					1107:1116	The proportion	1103:1116	The proportion of CD25+Foxp3+CD4+ regulatory T cell (Foxp3+ Treg) in the pancreas, pancreatic and mesenteric lymph nodes	1103:1222	The proportion of CD25+Foxp3+CD4+ regulatory T cell (Foxp3+ Treg) in the pancreas, pancreatic and mesenteric lymph nodes was analyzed by flow cytometry.
31417546	9	90	theme	fasting	1402:1408	arg1	levels					1418:1423	fasting glucose levels	1402:1423	fasting glucose levels	1402:1423	We found that LMP supplementation ameliorated T1D development in non-obese diabetic (NOD) mice, as evidenced by decreasing diabetes incidence and fasting glucose levels in LMP fed NOD mice.
31417546	1	91	dep	manipulations	194:206	arg1	enhance					211:217	enhance	211:217	to enhance intestinal homeostasis	208:240	Intestinal homeostasis underpins the development of type 1 diabetes (T1D), and dietary manipulations to enhance intestinal homeostasis have been proposed to prevent T1D.
31417546	12	92	theme	LMP	1989:1991	arg1	effect					1979:1984	The microbiota-dependent beneficial effect	1943:1984	The microbiota-dependent beneficial effect of LMP on T1D	1943:1998	The microbiota-dependent beneficial effect of LMP on T1D was further proven by the fact that aberration of caecal microbiota by ABX treatment worsened T1D autoimmunity and could be restored with transfer of feces of LMP-fed NOD mice.
31417546	8	93	theme	mesenteric	1201:1210	arg1	nodes					1218:1222	mesenteric lymph nodes	1201:1222	mesenteric lymph nodes	1201:1222	The proportion of CD25+Foxp3+CD4+ regulatory T cell (Foxp3+ Treg) in the pancreas, pancreatic and mesenteric lymph nodes was analyzed by flow cytometry.
31417546	10	94	theme	caecal	1575:1580	arg1	species					1592:1598	selectively enriched caecal bacterial species	1554:1598	selectively enriched caecal bacterial species	1554:1598	Further microbiota analysis revealed that LMP supplementation prevented T1D-associated caecal dysbiosis and selectively enriched caecal bacterial species to produce more SCFAs.
31417546	8	95	from	pancreas	1176:1183	arg1	proportion					1107:1116	The proportion	1103:1116	The proportion of CD25+Foxp3+CD4+ regulatory T cell (Foxp3+ Treg) in the pancreas, pancreatic and mesenteric lymph nodes	1103:1222	The proportion of CD25+Foxp3+CD4+ regulatory T cell (Foxp3+ Treg) in the pancreas, pancreatic and mesenteric lymph nodes was analyzed by flow cytometry.
31417546	13	96	theme	caecal	2255:2260	arg1	homeostasis					2262:2272	caecal homeostasis	2255:2272	caecal homeostasis	2255:2272	These data demonstrate that this novel LMP limits T1D development by inducing caecal homeostasis to shape pancreatic immune environment.
31417546	6	97	theme	acids	889:893	arg1	Concentration					854:866	Concentration	854:866	Concentration of short chain fatty acids	854:893	Concentration of short chain fatty acids were determined by GC-MS.
31417546	8	98	theme	nodes	1218:1222	arg1	proportion					1107:1116	The proportion	1103:1116	The proportion of CD25+Foxp3+CD4+ regulatory T cell (Foxp3+ Treg) in the pancreas, pancreatic and mesenteric lymph nodes	1103:1222	The proportion of CD25+Foxp3+CD4+ regulatory T cell (Foxp3+ Treg) in the pancreas, pancreatic and mesenteric lymph nodes was analyzed by flow cytometry.
31417546	3	99	theme	diabetes	545:552	arg1	incidence					554:562	overt diabetes incidence	539:562	overt diabetes incidence	539:562	Female NOD mice were weaned onto control or 5% (wt/wt) LMP supplemented diets for up to 40 weeks of age, overt diabetes incidence and blood glucose were monitored.
31417546	7	100	theme	zonula	969:974	arg1	ZO-2					989:992	ZO-2	989:992	ZO-2	989:992	The barrier reinforcing tight junction proteins zonula occludens-2 (ZO-2), claudin-1 and NOD like receptor protein 3 (NLRP3) inflammasome activation were determined by Western blot.
31417546	7	100	theme	zonula	969:974	arg1	occludens-2					976:986	zonula occludens-2	969:986	zonula occludens-2 (ZO-2)	969:993	The barrier reinforcing tight junction proteins zonula occludens-2 (ZO-2), claudin-1 and NOD like receptor protein 3 (NLRP3) inflammasome activation were determined by Western blot.
31417546	7	100	theme	zonula	969:974	arg1	proteins					960:967	The barrier reinforcing tight junction proteins	921:967	The barrier reinforcing tight junction proteins zonula occludens-2 (ZO-2), claudin-1 and NOD like receptor protein 3 (NLRP3) inflammasome activation	921:1068	The barrier reinforcing tight junction proteins zonula occludens-2 (ZO-2), claudin-1 and NOD like receptor protein 3 (NLRP3) inflammasome activation were determined by Western blot.
31417546	9	101	theme	LMP	1428:1430	arg1	mice					1440:1443	LMP fed NOD mice	1428:1443	LMP fed NOD mice	1428:1443	We found that LMP supplementation ameliorated T1D development in non-obese diabetic (NOD) mice, as evidenced by decreasing diabetes incidence and fasting glucose levels in LMP fed NOD mice.
31417546	12	102	theme	ABX	2071:2073	arg1	treatment					2075:2083	ABX treatment	2071:2083	ABX treatment	2071:2083	The microbiota-dependent beneficial effect of LMP on T1D was further proven by the fact that aberration of caecal microbiota by ABX treatment worsened T1D autoimmunity and could be restored with transfer of feces of LMP-fed NOD mice.
31417546	0	103	theme	Type	63:66	arg1	Diabetes					70:77	Type 1 Diabetes	63:77	Type 1 Diabetes in Non-obese Diabetic Mice	63:104	Modulation of Gut Microbiota by Low Methoxyl Pectin Attenuates Type 1 Diabetes in Non-obese Diabetic Mice.
31417546	7	104	theme	junction	951:958	arg1	NOD					1010:1012	NOD	1010:1012	NOD	1010:1012	The barrier reinforcing tight junction proteins zonula occludens-2 (ZO-2), claudin-1 and NOD like receptor protein 3 (NLRP3) inflammasome activation were determined by Western blot.
31417546	7	104	theme	junction	951:958	arg1	claudin-1					996:1004	claudin-1	996:1004	claudin-1	996:1004	The barrier reinforcing tight junction proteins zonula occludens-2 (ZO-2), claudin-1 and NOD like receptor protein 3 (NLRP3) inflammasome activation were determined by Western blot.
31417546	7	104	theme	junction	951:958	arg1	occludens-2					976:986	zonula occludens-2	969:986	zonula occludens-2 (ZO-2)	969:993	The barrier reinforcing tight junction proteins zonula occludens-2 (ZO-2), claudin-1 and NOD like receptor protein 3 (NLRP3) inflammasome activation were determined by Western blot.
31417546	7	104	theme	junction	951:958	arg1	proteins					960:967	The barrier reinforcing tight junction proteins	921:967	The barrier reinforcing tight junction proteins zonula occludens-2 (ZO-2), claudin-1 and NOD like receptor protein 3 (NLRP3) inflammasome activation	921:1068	The barrier reinforcing tight junction proteins zonula occludens-2 (ZO-2), claudin-1 and NOD like receptor protein 3 (NLRP3) inflammasome activation were determined by Western blot.
31417546	9	105	theme	NOD	1436:1438	arg1	mice					1440:1443	LMP fed NOD mice	1428:1443	LMP fed NOD mice	1428:1443	We found that LMP supplementation ameliorated T1D development in non-obese diabetic (NOD) mice, as evidenced by decreasing diabetes incidence and fasting glucose levels in LMP fed NOD mice.
31417546	8	106	theme	flow	1240:1243	arg1	cytometry					1245:1253	flow cytometry	1240:1253	flow cytometry	1240:1253	The proportion of CD25+Foxp3+CD4+ regulatory T cell (Foxp3+ Treg) in the pancreas, pancreatic and mesenteric lymph nodes was analyzed by flow cytometry.
31417546	11	107	theme	immune	1725:1730	arg1	environment					1732:1742	shaped gut-pancreatic immune environment	1703:1742	shaped gut-pancreatic immune environment	1703:1742	The LMP-mediated microbial balance further enhanced caecal barrier function and shaped gut-pancreatic immune environment, as characterized by higher expression of tight junction proteins claudin-1, ZO-2 in caecum, increased Foxp3+ Treg population and decreased NLRP3 inflammasome activation in both caecum and pancreas.
31417546	7	108	theme	reinforcing	933:943	arg1	NOD					1010:1012	NOD	1010:1012	NOD	1010:1012	The barrier reinforcing tight junction proteins zonula occludens-2 (ZO-2), claudin-1 and NOD like receptor protein 3 (NLRP3) inflammasome activation were determined by Western blot.
31417546	7	108	theme	reinforcing	933:943	arg1	claudin-1					996:1004	claudin-1	996:1004	claudin-1	996:1004	The barrier reinforcing tight junction proteins zonula occludens-2 (ZO-2), claudin-1 and NOD like receptor protein 3 (NLRP3) inflammasome activation were determined by Western blot.
31417546	7	108	theme	reinforcing	933:943	arg1	occludens-2					976:986	zonula occludens-2	969:986	zonula occludens-2 (ZO-2)	969:993	The barrier reinforcing tight junction proteins zonula occludens-2 (ZO-2), claudin-1 and NOD like receptor protein 3 (NLRP3) inflammasome activation were determined by Western blot.
31417546	7	108	theme	reinforcing	933:943	arg1	proteins					960:967	The barrier reinforcing tight junction proteins	921:967	The barrier reinforcing tight junction proteins zonula occludens-2 (ZO-2), claudin-1 and NOD like receptor protein 3 (NLRP3) inflammasome activation	921:1068	The barrier reinforcing tight junction proteins zonula occludens-2 (ZO-2), claudin-1 and NOD like receptor protein 3 (NLRP3) inflammasome activation were determined by Western blot.
31417546	11	109	theme	higher	1765:1770	arg1	expression					1772:1781	higher expression	1765:1781	higher expression of tight junction proteins claudin-1, ZO-2 in caecum, increased Foxp3+ Treg population and decreased NLRP3 inflammasome activation in both caecum and pancreas	1765:1940	The LMP-mediated microbial balance further enhanced caecal barrier function and shaped gut-pancreatic immune environment, as characterized by higher expression of tight junction proteins claudin-1, ZO-2 in caecum, increased Foxp3+ Treg population and decreased NLRP3 inflammasome activation in both caecum and pancreas.
31417546	0	110	theme	Non-obese	82:90	arg1	Mice					101:104	Non-obese Diabetic Mice	82:104	Non-obese Diabetic Mice	82:104	Modulation of Gut Microbiota by Low Methoxyl Pectin Attenuates Type 1 Diabetes in Non-obese Diabetic Mice.
31417546	11	111	from	population	1859:1868	arg1	caecum					1829:1834	caecum	1829:1834	caecum	1829:1834	The LMP-mediated microbial balance further enhanced caecal barrier function and shaped gut-pancreatic immune environment, as characterized by higher expression of tight junction proteins claudin-1, ZO-2 in caecum, increased Foxp3+ Treg population and decreased NLRP3 inflammasome activation in both caecum and pancreas.
31417546	4	112	theme	gut	676:678	arg1	transfer					691:698	gut microbiota transfer	676:698	gut microbiota transfer	676:698	Then broad-spectrum antibiotics (ABX) treatment per os for 7 days followed by gut microbiota transfer was performed to demonstrate gut microbiota-dependent effects.
31417546	7	113	dep	proteins	960:967	arg1	NOD					1010:1012	NOD	1010:1012	NOD	1010:1012	The barrier reinforcing tight junction proteins zonula occludens-2 (ZO-2), claudin-1 and NOD like receptor protein 3 (NLRP3) inflammasome activation were determined by Western blot.
31417546	7	113	dep	proteins	960:967	arg1	ZO-2					989:992	ZO-2	989:992	ZO-2	989:992	The barrier reinforcing tight junction proteins zonula occludens-2 (ZO-2), claudin-1 and NOD like receptor protein 3 (NLRP3) inflammasome activation were determined by Western blot.
31417546	7	113	dep	proteins	960:967	arg1	claudin-1					996:1004	claudin-1	996:1004	claudin-1	996:1004	The barrier reinforcing tight junction proteins zonula occludens-2 (ZO-2), claudin-1 and NOD like receptor protein 3 (NLRP3) inflammasome activation were determined by Western blot.
31417546	7	113	dep	proteins	960:967	arg1	occludens-2					976:986	zonula occludens-2	969:986	zonula occludens-2 (ZO-2)	969:993	The barrier reinforcing tight junction proteins zonula occludens-2 (ZO-2), claudin-1 and NOD like receptor protein 3 (NLRP3) inflammasome activation were determined by Western blot.
31417546	7	113	dep	proteins	960:967	arg1	proteins					960:967	The barrier reinforcing tight junction proteins	921:967	The barrier reinforcing tight junction proteins zonula occludens-2 (ZO-2), claudin-1 and NOD like receptor protein 3 (NLRP3) inflammasome activation	921:1068	The barrier reinforcing tight junction proteins zonula occludens-2 (ZO-2), claudin-1 and NOD like receptor protein 3 (NLRP3) inflammasome activation were determined by Western blot.
31417546	8	114	from	nodes	1218:1222	arg1	pancreas					1176:1183	the pancreas	1172:1183	the pancreas	1172:1183	The proportion of CD25+Foxp3+CD4+ regulatory T cell (Foxp3+ Treg) in the pancreas, pancreatic and mesenteric lymph nodes was analyzed by flow cytometry.
31417546	10	115	theme	more	1611:1614	arg1	SCFAs					1616:1620	more SCFAs	1611:1620	more SCFAs	1611:1620	Further microbiota analysis revealed that LMP supplementation prevented T1D-associated caecal dysbiosis and selectively enriched caecal bacterial species to produce more SCFAs.
31417546	2	116	theme	T1D	417:419	arg1	development					421:431	T1D development	417:431	T1D development	417:431	The current study aimed to investigate the efficacy of supplementing a novel specific low-methoxyl pectin (LMP) dietary fiber in preventing T1D development.
31417546	2	117	theme	specific	354:361	arg1	pectin					376:381	specific low-methoxyl pectin	354:381	a novel specific low-methoxyl pectin (LMP) dietary fiber	346:401	The current study aimed to investigate the efficacy of supplementing a novel specific low-methoxyl pectin (LMP) dietary fiber in preventing T1D development.
31417546	2	117	theme	specific	354:361	arg1	LMP					384:386	LMP	384:386	LMP	384:386	The current study aimed to investigate the efficacy of supplementing a novel specific low-methoxyl pectin (LMP) dietary fiber in preventing T1D development.
31417546	0	118	theme	Microbiota	18:27	arg1	Modulation					0:9	Modulation	0:9	Modulation of Gut Microbiota by Low Methoxyl Pectin	0:50	Modulation of Gut Microbiota by Low Methoxyl Pectin Attenuates Type 1 Diabetes in Non-obese Diabetic Mice.
31417546	13	119	theme	novel	2210:2214	arg1	LMP					2216:2218	this novel LMP	2205:2218	this novel LMP	2205:2218	These data demonstrate that this novel LMP limits T1D development by inducing caecal homeostasis to shape pancreatic immune environment.
31417546	2	120	theme	dietary	389:395	arg1	fiber					397:401	a novel specific low-methoxyl pectin (LMP) dietary fiber	346:401	a novel specific low-methoxyl pectin (LMP) dietary fiber	346:401	The current study aimed to investigate the efficacy of supplementing a novel specific low-methoxyl pectin (LMP) dietary fiber in preventing T1D development.
31417546	7	121	theme	Western	1089:1095	arg1	blot					1097:1100	Western blot	1089:1100	Western blot	1089:1100	The barrier reinforcing tight junction proteins zonula occludens-2 (ZO-2), claudin-1 and NOD like receptor protein 3 (NLRP3) inflammasome activation were determined by Western blot.
31417546	4	122	theme	microbiota-dependent	733:752	arg1	effects					754:760	gut microbiota-dependent effects	729:760	gut microbiota-dependent effects	729:760	Then broad-spectrum antibiotics (ABX) treatment per os for 7 days followed by gut microbiota transfer was performed to demonstrate gut microbiota-dependent effects.
31417546	3	123	theme	Female	434:439	arg1	mice					445:448	Female NOD mice	434:448	Female NOD mice	434:448	Female NOD mice were weaned onto control or 5% (wt/wt) LMP supplemented diets for up to 40 weeks of age, overt diabetes incidence and blood glucose were monitored.
31417546	9	124	theme	T1D	1302:1304	arg1	development					1306:1316	T1D development	1302:1316	T1D development	1302:1316	We found that LMP supplementation ameliorated T1D development in non-obese diabetic (NOD) mice, as evidenced by decreasing diabetes incidence and fasting glucose levels in LMP fed NOD mice.
31417546	6	125	theme	chain	877:881	arg1	acids					889:893	short chain fatty acids	871:893	short chain fatty acids	871:893	Concentration of short chain fatty acids were determined by GC-MS.
31417546	12	126	theme	mice	2171:2174	arg1	feces					2150:2154	feces	2150:2154	feces of LMP-fed NOD mice	2150:2174	The microbiota-dependent beneficial effect of LMP on T1D was further proven by the fact that aberration of caecal microbiota by ABX treatment worsened T1D autoimmunity and could be restored with transfer of feces of LMP-fed NOD mice.
31417546	11	127	theme	increased	1837:1845	arg1	population					1859:1868	increased Foxp3+ Treg population	1837:1868	increased Foxp3+ Treg population	1837:1868	The LMP-mediated microbial balance further enhanced caecal barrier function and shaped gut-pancreatic immune environment, as characterized by higher expression of tight junction proteins claudin-1, ZO-2 in caecum, increased Foxp3+ Treg population and decreased NLRP3 inflammasome activation in both caecum and pancreas.
31417546	11	127	theme	increased	1837:1845	arg1	proteins					1801:1808	tight junction proteins claudin-1, ZO-2 in caecum, increased Foxp3+ Treg population and decreased NLRP3 inflammasome activation	1786:1912	tight junction proteins claudin-1, ZO-2 in caecum, increased Foxp3+ Treg population and decreased NLRP3 inflammasome activation	1786:1912	The LMP-mediated microbial balance further enhanced caecal barrier function and shaped gut-pancreatic immune environment, as characterized by higher expression of tight junction proteins claudin-1, ZO-2 in caecum, increased Foxp3+ Treg population and decreased NLRP3 inflammasome activation in both caecum and pancreas.
31417546	12	128	theme	feces	2150:2154	arg1	transfer					2138:2145	transfer	2138:2145	transfer of feces of LMP-fed NOD mice	2138:2174	The microbiota-dependent beneficial effect of LMP on T1D was further proven by the fact that aberration of caecal microbiota by ABX treatment worsened T1D autoimmunity and could be restored with transfer of feces of LMP-fed NOD mice.
31417546	8	129	from	proportion	1107:1116	arg1	pancreas					1176:1183	the pancreas	1172:1183	the pancreas	1172:1183	The proportion of CD25+Foxp3+CD4+ regulatory T cell (Foxp3+ Treg) in the pancreas, pancreatic and mesenteric lymph nodes was analyzed by flow cytometry.
31417546	11	130	theme	barrier	1682:1688	arg1	function					1690:1697	caecal barrier function	1675:1697	caecal barrier function	1675:1697	The LMP-mediated microbial balance further enhanced caecal barrier function and shaped gut-pancreatic immune environment, as characterized by higher expression of tight junction proteins claudin-1, ZO-2 in caecum, increased Foxp3+ Treg population and decreased NLRP3 inflammasome activation in both caecum and pancreas.
31417546	9	131	theme	diabetic	1331:1338	arg1	mice					1346:1349	non-obese diabetic (NOD) mice	1321:1349	non-obese diabetic (NOD) mice	1321:1349	We found that LMP supplementation ameliorated T1D development in non-obese diabetic (NOD) mice, as evidenced by decreasing diabetes incidence and fasting glucose levels in LMP fed NOD mice.
31417546	1	132	theme	dietary	186:192	arg1	manipulations					194:206	dietary manipulations	186:206	dietary manipulations to enhance intestinal homeostasis	186:240	Intestinal homeostasis underpins the development of type 1 diabetes (T1D), and dietary manipulations to enhance intestinal homeostasis have been proposed to prevent T1D.
31417546	2	133	theme	pectin	376:381	arg1	fiber					397:401	a novel specific low-methoxyl pectin (LMP) dietary fiber	346:401	a novel specific low-methoxyl pectin (LMP) dietary fiber	346:401	The current study aimed to investigate the efficacy of supplementing a novel specific low-methoxyl pectin (LMP) dietary fiber in preventing T1D development.
31417546	1	134	theme	intestinal	219:228	arg1	homeostasis					230:240	intestinal homeostasis	219:240	intestinal homeostasis	219:240	Intestinal homeostasis underpins the development of type 1 diabetes (T1D), and dietary manipulations to enhance intestinal homeostasis have been proposed to prevent T1D.
31417546	14	135	theme	T1D	2402:2404	arg1	manipulation					2371:2382	gut microbiota manipulation	2356:2382	gut microbiota manipulation	2356:2382	This finding opens a realistic option for gut microbiota manipulation and prevention of T1D in humans.
31417546	14	135	theme	T1D	2402:2404	arg1	prevention					2388:2397	prevention	2388:2397	prevention of T1D in humans	2388:2414	This finding opens a realistic option for gut microbiota manipulation and prevention of T1D in humans.
31417546	9	136	theme	NOD	1341:1343	arg1	mice					1346:1349	non-obese diabetic (NOD) mice	1321:1349	non-obese diabetic (NOD) mice	1321:1349	We found that LMP supplementation ameliorated T1D development in non-obese diabetic (NOD) mice, as evidenced by decreasing diabetes incidence and fasting glucose levels in LMP fed NOD mice.
29807527	11	0	from	translation	1858:1868	arg1	EVs					1891:1893	EVs	1891:1893	EVs from LPS	1891:1902	According to Gene Ontology (GO) analysis, we found a large increase of proteins related to translation and transcription in EVs from LPS.
29807527	11	0	from	translation	1858:1868	arg1	LPS					1900:1902	LPS	1900:1902	LPS	1900:1902	According to Gene Ontology (GO) analysis, we found a large increase of proteins related to translation and transcription in EVs from LPS.
29807527	4	1	theme	inflammatory	565:576	arg1	conditions					578:587	inflammatory conditions	565:587	inflammatory conditions	565:587	Here, we characterized microglial EVs under inflammatory conditions and investigated the effects of inflammation on the EV size, quantity, and protein content.
29807527	14	2	theme	potential	2238:2246	arg1	roles					2248:2252	the potential roles	2234:2252	the potential roles of EVs	2234:2259	Our findings provide new insights into the potential roles of EVs that could be related to the pathogenesis in neuroinflammatory diseases.
29807527	2	3	theme	cell-to-cell	351:362	arg1	communication					364:376	cell-to-cell communication	351:376	cell-to-cell communication	351:376	Microglia-derived extracellular vesicles (EVs) are suggested to be involved in propagation of inflammatory signals and in the modulation of cell-to-cell communication.
29807527	11	4	from	transcription	1874:1886	arg1	EVs					1891:1893	EVs	1891:1893	EVs from LPS	1891:1902	According to Gene Ontology (GO) analysis, we found a large increase of proteins related to translation and transcription in EVs from LPS.
29807527	11	4	from	transcription	1874:1886	arg1	LPS					1900:1902	LPS	1900:1902	LPS	1900:1902	According to Gene Ontology (GO) analysis, we found a large increase of proteins related to translation and transcription in EVs from LPS.
29807527	1	5	theme	Activated	96:104	arg1	microglia					106:114	BACKGROUND Activated microglia	85:114	BACKGROUND Activated microglia	85:114	BACKGROUND Activated microglia play an essential role in inflammatory responses elicited in the central nervous system (CNS).
29807527	13	6	from	composition	2138:2148	arg1	cells					2092:2096	BV2 microglial cells	2077:2096	BV2 microglial cells	2077:2096	CONCLUSIONS We demonstrate altered EV production in BV2 microglial cells and altered cytokine levels and protein composition carried by EVs in response to LPS challenge.
29807527	5	7	theme	secreted	831:838	arg1	EVs					840:842	secreted EVs	831:842	secreted EVs from a microglial cell line (BV2) following lipopolysaccharide (LPS) and tumor necrosis factor (TNF) inhibitor (etanercept) treatments	831:977	METHODS We have utilized western blot, nanoparticle tracking analysis (NTA), and mass spectrometry to characterize EVs and examine the alterations of secreted EVs from a microglial cell line (BV2) following lipopolysaccharide (LPS) and tumor necrosis factor (TNF) inhibitor (etanercept) treatments, or either alone.
29807527	7	8	from	effect	1208:1213	arg1	release					1239:1245	EV release	1236:1245	EV release	1236:1245	We also subjected TNF knockout mice to experimental stroke (permanent middle cerebral artery occlusion) and validated the effect of TNF inhibition on EV release.
29807527	2	9	theme	signals	318:324	arg1	propagation					290:300	propagation	290:300	propagation of inflammatory signals	290:324	Microglia-derived extracellular vesicles (EVs) are suggested to be involved in propagation of inflammatory signals and in the modulation of cell-to-cell communication.
29807527	12	10	theme	proteins	1950:1957	arg1	profile					1939:1945	a distinct profile	1928:1945	a distinct profile of proteins found in EVs released from LPS treated cells compared to control	1928:2022	Importantly, we showed a distinct profile of proteins found in EVs released from LPS treated cells compared to control.
29807527	5	11	used	utilized	697:704	arg2	We					689:690	We	689:690	We	689:690	METHODS We have utilized western blot, nanoparticle tracking analysis (NTA), and mass spectrometry to characterize EVs and examine the alterations of secreted EVs from a microglial cell line (BV2) following lipopolysaccharide (LPS) and tumor necrosis factor (TNF) inhibitor (etanercept) treatments, or either alone.
29807527	8	12	theme	TNF	1381:1383	arg1	levels					1371:1376	the intravesicular levels	1352:1376	the intravesicular levels of TNF and interleukin (IL)-6	1352:1406	RESULTS Our analysis of EVs originating from activated BV2 microglia revealed a significant increase in the intravesicular levels of TNF and interleukin (IL)-6.
29807527	4	13	theme	EV	641:642	arg1	size					644:647	the EV size	637:647	the EV size	637:647	Here, we characterized microglial EVs under inflammatory conditions and investigated the effects of inflammation on the EV size, quantity, and protein content.
29807527	11	14	theme	large	1820:1824	arg1	increase					1826:1833	a large increase	1818:1833	a large increase of proteins related to translation and transcription in EVs from LPS	1818:1902	According to Gene Ontology (GO) analysis, we found a large increase of proteins related to translation and transcription in EVs from LPS.
29807527	8	15	theme	interleukin	1389:1399	arg1	levels					1371:1376	the intravesicular levels	1352:1376	the intravesicular levels of TNF and interleukin (IL)-6	1352:1406	RESULTS Our analysis of EVs originating from activated BV2 microglia revealed a significant increase in the intravesicular levels of TNF and interleukin (IL)-6.
29807527	2	16	theme	extracellular	229:241	arg1	EVs					253:255	EVs	253:255	EVs	253:255	Microglia-derived extracellular vesicles (EVs) are suggested to be involved in propagation of inflammatory signals and in the modulation of cell-to-cell communication.
29807527	2	16	theme	extracellular	229:241	arg1	vesicles					243:250	Microglia-derived extracellular vesicles	211:250	Microglia-derived extracellular vesicles (EVs)	211:256	Microglia-derived extracellular vesicles (EVs) are suggested to be involved in propagation of inflammatory signals and in the modulation of cell-to-cell communication.
29807527	13	17	theme	protein	2130:2136	arg1	composition					2138:2148	protein composition	2130:2148	protein composition	2130:2148	CONCLUSIONS We demonstrate altered EV production in BV2 microglial cells and altered cytokine levels and protein composition carried by EVs in response to LPS challenge.
29807527	11	18	dep	Ontology	1785:1792	arg1	GO					1795:1796	GO	1795:1796	GO	1795:1796	According to Gene Ontology (GO) analysis, we found a large increase of proteins related to translation and transcription in EVs from LPS.
29807527	13	19	from	levels	2119:2124	arg1	cells					2092:2096	BV2 microglial cells	2077:2096	BV2 microglial cells	2077:2096	CONCLUSIONS We demonstrate altered EV production in BV2 microglial cells and altered cytokine levels and protein composition carried by EVs in response to LPS challenge.
29807527	10	20	theme	LPS-activated	1717:1729	arg1	microglia					1731:1739	LPS-activated microglia	1717:1739	LPS-activated microglia	1717:1739	Finally, via mass spectrometry, we identified 49 unique proteins in EVs released from LPS-activated microglia compared to control EVs (58 proteins in EVs released from LPS-activated microglia and 37 from control EVs).
29807527	1	21	theme	inflammatory	142:153	arg1	responses					155:163	inflammatory responses	142:163	inflammatory responses elicited in the central nervous system (CNS)	142:208	BACKGROUND Activated microglia play an essential role in inflammatory responses elicited in the central nervous system (CNS).
29807527	4	22	theme	protein	664:670	arg1	content					672:678	protein content	664:678	protein content	664:678	Here, we characterized microglial EVs under inflammatory conditions and investigated the effects of inflammation on the EV size, quantity, and protein content.
29807527	7	23	theme	artery	1172:1177	arg1	stroke					1138:1143	experimental stroke	1125:1143	experimental stroke (permanent middle cerebral artery occlusion)	1125:1188	We also subjected TNF knockout mice to experimental stroke (permanent middle cerebral artery occlusion) and validated the effect of TNF inhibition on EV release.
29807527	7	23	theme	artery	1172:1177	arg1	occlusion					1179:1187	permanent middle cerebral artery occlusion	1146:1187	permanent middle cerebral artery occlusion	1146:1187	We also subjected TNF knockout mice to experimental stroke (permanent middle cerebral artery occlusion) and validated the effect of TNF inhibition on EV release.
29807527	5	24	theme	microglial	851:860	arg1	BV2					873:875	BV2	873:875	BV2	873:875	METHODS We have utilized western blot, nanoparticle tracking analysis (NTA), and mass spectrometry to characterize EVs and examine the alterations of secreted EVs from a microglial cell line (BV2) following lipopolysaccharide (LPS) and tumor necrosis factor (TNF) inhibitor (etanercept) treatments, or either alone.
29807527	5	24	theme	microglial	851:860	arg1	line					867:870	a microglial cell line	849:870	a microglial cell line (BV2) following lipopolysaccharide (LPS) and tumor necrosis factor (TNF) inhibitor (etanercept) treatments	849:977	METHODS We have utilized western blot, nanoparticle tracking analysis (NTA), and mass spectrometry to characterize EVs and examine the alterations of secreted EVs from a microglial cell line (BV2) following lipopolysaccharide (LPS) and tumor necrosis factor (TNF) inhibitor (etanercept) treatments, or either alone.
29807527	5	25	dep	METHODS	681:687	arg1	utilized					697:704	utilized	697:704	have utilized western blot, nanoparticle tracking analysis (NTA), and mass spectrometry to characterize EVs and examine the alterations of secreted EVs from a microglial cell line (BV2) following lipopolysaccharide (LPS) and tumor necrosis factor (TNF) inhibitor (etanercept) treatments, or either alone	692:994	METHODS We have utilized western blot, nanoparticle tracking analysis (NTA), and mass spectrometry to characterize EVs and examine the alterations of secreted EVs from a microglial cell line (BV2) following lipopolysaccharide (LPS) and tumor necrosis factor (TNF) inhibitor (etanercept) treatments, or either alone.
29807527	7	26	theme	middle	1156:1161	arg1	stroke					1138:1143	experimental stroke	1125:1143	experimental stroke (permanent middle cerebral artery occlusion)	1125:1188	We also subjected TNF knockout mice to experimental stroke (permanent middle cerebral artery occlusion) and validated the effect of TNF inhibition on EV release.
29807527	7	26	theme	middle	1156:1161	arg1	occlusion					1179:1187	permanent middle cerebral artery occlusion	1146:1187	permanent middle cerebral artery occlusion	1146:1187	We also subjected TNF knockout mice to experimental stroke (permanent middle cerebral artery occlusion) and validated the effect of TNF inhibition on EV release.
29807527	9	27	theme	EVs	1445:1447	arg1	number					1435:1440	the number	1431:1440	the number of EVs released	1431:1456	We also observed that the number of EVs released was reduced both in vitro and in vivo when inflammation was inhibited via the TNF pathway.
29807527	11	28	theme	related	1847:1853	arg1	proteins					1838:1845	proteins	1838:1845	proteins related to translation and transcription in EVs from LPS	1838:1902	According to Gene Ontology (GO) analysis, we found a large increase of proteins related to translation and transcription in EVs from LPS.
29807527	3	29	dep	is	394:395	arg1	facilitates					463:473	facilitates	463:473	facilitates the communication between cells in the brain	463:518	However, there is a lack of knowledge on the regulation of EVs and how this in turn facilitates the communication between cells in the brain.
29807527	8	30	theme	EVs	1272:1274	arg1	analysis					1260:1267	Our analysis	1256:1267	Our analysis of EVs originating from activated BV2 microglia	1256:1315	RESULTS Our analysis of EVs originating from activated BV2 microglia revealed a significant increase in the intravesicular levels of TNF and interleukin (IL)-6.
29807527	5	31	theme	tumor	917:921	arg1	inhibitor					945:953	tumor necrosis factor (TNF) inhibitor	917:953	tumor necrosis factor (TNF) inhibitor	917:953	METHODS We have utilized western blot, nanoparticle tracking analysis (NTA), and mass spectrometry to characterize EVs and examine the alterations of secreted EVs from a microglial cell line (BV2) following lipopolysaccharide (LPS) and tumor necrosis factor (TNF) inhibitor (etanercept) treatments, or either alone.
29807527	10	32	from	proteins	1687:1694	arg1	EVs					1699:1701	EVs	1699:1701	EVs released from LPS-activated microglia	1699:1739	Finally, via mass spectrometry, we identified 49 unique proteins in EVs released from LPS-activated microglia compared to control EVs (58 proteins in EVs released from LPS-activated microglia and 37 from control EVs).
29807527	12	33	theme	distinct	1930:1937	arg1	profile					1939:1945	a distinct profile	1928:1945	a distinct profile of proteins found in EVs released from LPS treated cells compared to control	1928:2022	Importantly, we showed a distinct profile of proteins found in EVs released from LPS treated cells compared to control.
29807527	14	34	from	pathogenesis	2290:2301	arg1	diseases					2324:2331	neuroinflammatory diseases	2306:2331	neuroinflammatory diseases	2306:2331	Our findings provide new insights into the potential roles of EVs that could be related to the pathogenesis in neuroinflammatory diseases.
29807527	11	35	theme	Ontology	1785:1792	arg1	analysis					1799:1806	Gene Ontology (GO) analysis	1780:1806	Gene Ontology (GO) analysis	1780:1806	According to Gene Ontology (GO) analysis, we found a large increase of proteins related to translation and transcription in EVs from LPS.
29807527	5	36	theme	nanoparticle	720:731	arg1	analysis					742:749	nanoparticle tracking analysis	720:749	nanoparticle tracking analysis (NTA)	720:755	METHODS We have utilized western blot, nanoparticle tracking analysis (NTA), and mass spectrometry to characterize EVs and examine the alterations of secreted EVs from a microglial cell line (BV2) following lipopolysaccharide (LPS) and tumor necrosis factor (TNF) inhibitor (etanercept) treatments, or either alone.
29807527	5	36	theme	nanoparticle	720:731	arg1	NTA					752:754	NTA	752:754	NTA	752:754	METHODS We have utilized western blot, nanoparticle tracking analysis (NTA), and mass spectrometry to characterize EVs and examine the alterations of secreted EVs from a microglial cell line (BV2) following lipopolysaccharide (LPS) and tumor necrosis factor (TNF) inhibitor (etanercept) treatments, or either alone.
29807527	1	37	theme	BACKGROUND	85:94	arg1	microglia					106:114	BACKGROUND Activated microglia	85:114	BACKGROUND Activated microglia	85:114	BACKGROUND Activated microglia play an essential role in inflammatory responses elicited in the central nervous system (CNS).
29807527	6	38	theme	inflammatory	1001:1012	arg1	responses					1014:1022	The inflammatory responses	997:1022	The inflammatory responses	997:1022	The inflammatory responses were measured with multiplex cytokine ELISA and western blot.
29807527	0	39	from	populations	31:41	arg1	microglia					74:82	microglia	74:82	microglia	74:82	Inflammation leads to distinct populations of extracellular vesicles from microglia.
29807527	5	40	theme	tracking	733:740	arg1	analysis					742:749	nanoparticle tracking analysis	720:749	nanoparticle tracking analysis (NTA)	720:755	METHODS We have utilized western blot, nanoparticle tracking analysis (NTA), and mass spectrometry to characterize EVs and examine the alterations of secreted EVs from a microglial cell line (BV2) following lipopolysaccharide (LPS) and tumor necrosis factor (TNF) inhibitor (etanercept) treatments, or either alone.
29807527	5	40	theme	tracking	733:740	arg1	NTA					752:754	NTA	752:754	NTA	752:754	METHODS We have utilized western blot, nanoparticle tracking analysis (NTA), and mass spectrometry to characterize EVs and examine the alterations of secreted EVs from a microglial cell line (BV2) following lipopolysaccharide (LPS) and tumor necrosis factor (TNF) inhibitor (etanercept) treatments, or either alone.
29807527	8	41	theme	BV2	1303:1305	arg1	microglia					1307:1315	activated BV2 microglia	1293:1315	activated BV2 microglia	1293:1315	RESULTS Our analysis of EVs originating from activated BV2 microglia revealed a significant increase in the intravesicular levels of TNF and interleukin (IL)-6.
29807527	7	42	theme	TNF	1104:1106	arg1	mice					1117:1120	TNF knockout mice	1104:1120	TNF knockout mice	1104:1120	We also subjected TNF knockout mice to experimental stroke (permanent middle cerebral artery occlusion) and validated the effect of TNF inhibition on EV release.
29807527	13	43	theme	microglial	2081:2090	arg1	cells					2092:2096	BV2 microglial cells	2077:2096	BV2 microglial cells	2077:2096	CONCLUSIONS We demonstrate altered EV production in BV2 microglial cells and altered cytokine levels and protein composition carried by EVs in response to LPS challenge.
29807527	3	44	theme	knowledge	407:415	arg1	lack					399:402	a lack	397:402	a lack of knowledge	397:415	However, there is a lack of knowledge on the regulation of EVs and how this in turn facilitates the communication between cells in the brain.
29807527	4	45	theme	microglial	544:553	arg1	EVs					555:557	microglial EVs	544:557	microglial EVs	544:557	Here, we characterized microglial EVs under inflammatory conditions and investigated the effects of inflammation on the EV size, quantity, and protein content.
29807527	2	46	link	Microglia-derived	211:227	arg1	EVs					253:255	EVs	253:255	EVs	253:255	Microglia-derived extracellular vesicles (EVs) are suggested to be involved in propagation of inflammatory signals and in the modulation of cell-to-cell communication.
29807527	2	46	link	Microglia-derived	211:227	arg1	vesicles					243:250	Microglia-derived extracellular vesicles	211:250	Microglia-derived extracellular vesicles (EVs)	211:256	Microglia-derived extracellular vesicles (EVs) are suggested to be involved in propagation of inflammatory signals and in the modulation of cell-to-cell communication.
29807527	8	47	theme	significant	1328:1338	arg1	increase					1340:1347	a significant increase	1326:1347	a significant increase in the intravesicular levels of TNF and interleukin (IL)-6	1326:1406	RESULTS Our analysis of EVs originating from activated BV2 microglia revealed a significant increase in the intravesicular levels of TNF and interleukin (IL)-6.
29807527	10	48	theme	mass	1562:1565	arg1	spectrometry					1567:1578	mass spectrometry	1562:1578	mass spectrometry	1562:1578	Finally, via mass spectrometry, we identified 49 unique proteins in EVs released from LPS-activated microglia compared to control EVs (58 proteins in EVs released from LPS-activated microglia and 37 from control EVs).
29807527	4	49	from	effects	610:616	arg1	content					672:678	protein content	664:678	protein content	664:678	Here, we characterized microglial EVs under inflammatory conditions and investigated the effects of inflammation on the EV size, quantity, and protein content.
29807527	4	49	from	effects	610:616	arg1	quantity					650:657	quantity	650:657	quantity	650:657	Here, we characterized microglial EVs under inflammatory conditions and investigated the effects of inflammation on the EV size, quantity, and protein content.
29807527	4	49	from	effects	610:616	arg1	size					644:647	the EV size	637:647	the EV size	637:647	Here, we characterized microglial EVs under inflammatory conditions and investigated the effects of inflammation on the EV size, quantity, and protein content.
29807527	10	50	theme	unique	1598:1603	arg1	proteins					1605:1612	49 unique proteins	1595:1612	49 unique proteins in EVs released from LPS-activated microglia	1595:1657	Finally, via mass spectrometry, we identified 49 unique proteins in EVs released from LPS-activated microglia compared to control EVs (58 proteins in EVs released from LPS-activated microglia and 37 from control EVs).
29807527	5	51	theme	mass	762:765	arg1	spectrometry					767:778	mass spectrometry	762:778	mass spectrometry	762:778	METHODS We have utilized western blot, nanoparticle tracking analysis (NTA), and mass spectrometry to characterize EVs and examine the alterations of secreted EVs from a microglial cell line (BV2) following lipopolysaccharide (LPS) and tumor necrosis factor (TNF) inhibitor (etanercept) treatments, or either alone.
29807527	13	52	theme	EV	2060:2061	arg1	production					2063:2072	altered EV production	2052:2072	altered EV production in BV2 microglial cells	2052:2096	CONCLUSIONS We demonstrate altered EV production in BV2 microglial cells and altered cytokine levels and protein composition carried by EVs in response to LPS challenge.
29807527	7	53	theme	TNF	1218:1220	arg1	inhibition					1222:1231	TNF inhibition	1218:1231	TNF inhibition	1218:1231	We also subjected TNF knockout mice to experimental stroke (permanent middle cerebral artery occlusion) and validated the effect of TNF inhibition on EV release.
29807527	14	54	theme	new	2216:2218	arg1	insights					2220:2227	new insights	2216:2227	new insights into the potential roles of EVs that could be related to the pathogenesis in neuroinflammatory diseases	2216:2331	Our findings provide new insights into the potential roles of EVs that could be related to the pathogenesis in neuroinflammatory diseases.
29807527	8	55	theme	intravesicular	1356:1369	arg1	levels					1371:1376	the intravesicular levels	1352:1376	the intravesicular levels of TNF and interleukin (IL)-6	1352:1406	RESULTS Our analysis of EVs originating from activated BV2 microglia revealed a significant increase in the intravesicular levels of TNF and interleukin (IL)-6.
29807527	5	56	theme	TNF	940:942	arg1	inhibitor					945:953	tumor necrosis factor (TNF) inhibitor	917:953	tumor necrosis factor (TNF) inhibitor	917:953	METHODS We have utilized western blot, nanoparticle tracking analysis (NTA), and mass spectrometry to characterize EVs and examine the alterations of secreted EVs from a microglial cell line (BV2) following lipopolysaccharide (LPS) and tumor necrosis factor (TNF) inhibitor (etanercept) treatments, or either alone.
29807527	6	57	theme	cytokine	1053:1060	arg1	ELISA					1062:1066	multiplex cytokine ELISA	1043:1066	multiplex cytokine ELISA	1043:1066	The inflammatory responses were measured with multiplex cytokine ELISA and western blot.
29807527	14	58	theme	EVs	2257:2259	arg1	roles					2248:2252	the potential roles	2234:2252	the potential roles of EVs	2234:2259	Our findings provide new insights into the potential roles of EVs that could be related to the pathogenesis in neuroinflammatory diseases.
29807527	1	59	theme	nervous	189:195	arg1	system					197:202	the central nervous system	177:202	the central nervous system (CNS)	177:208	BACKGROUND Activated microglia play an essential role in inflammatory responses elicited in the central nervous system (CNS).
29807527	1	59	theme	nervous	189:195	arg1	CNS					205:207	CNS	205:207	CNS	205:207	BACKGROUND Activated microglia play an essential role in inflammatory responses elicited in the central nervous system (CNS).
29807527	9	60	theme	TNF	1536:1538	arg1	pathway					1540:1546	the TNF pathway	1532:1546	the TNF pathway	1532:1546	We also observed that the number of EVs released was reduced both in vitro and in vivo when inflammation was inhibited via the TNF pathway.
29807527	13	61	theme	altered	2102:2108	arg1	levels					2119:2124	altered cytokine levels	2102:2124	altered cytokine levels	2102:2124	CONCLUSIONS We demonstrate altered EV production in BV2 microglial cells and altered cytokine levels and protein composition carried by EVs in response to LPS challenge.
29807527	2	62	theme	communication	364:376	arg1	modulation					337:346	the modulation	333:346	the modulation of cell-to-cell communication	333:376	Microglia-derived extracellular vesicles (EVs) are suggested to be involved in propagation of inflammatory signals and in the modulation of cell-to-cell communication.
29807527	12	63	attach	released	1972:1979	arg2	EVs					1968:1970	EVs	1968:1970	EVs released from LPS treated cells compared to control	1968:2022	Importantly, we showed a distinct profile of proteins found in EVs released from LPS treated cells compared to control.
29807527	12	63	attach	released	1972:1979	arg1	cells					1998:2002	LPS treated cells	1986:2002	LPS treated cells	1986:2002	Importantly, we showed a distinct profile of proteins found in EVs released from LPS treated cells compared to control.
29807527	10	64	from	proteins	1605:1612	arg1	EVs					1617:1619	EVs	1617:1619	EVs released from LPS-activated microglia	1617:1657	Finally, via mass spectrometry, we identified 49 unique proteins in EVs released from LPS-activated microglia compared to control EVs (58 proteins in EVs released from LPS-activated microglia and 37 from control EVs).
29807527	3	65	from	cells	501:505	arg1	brain					514:518	the brain	510:518	the brain	510:518	However, there is a lack of knowledge on the regulation of EVs and how this in turn facilitates the communication between cells in the brain.
29807527	14	66	theme	neuroinflammatory	2306:2322	arg1	diseases					2324:2331	neuroinflammatory diseases	2306:2331	neuroinflammatory diseases	2306:2331	Our findings provide new insights into the potential roles of EVs that could be related to the pathogenesis in neuroinflammatory diseases.
29807527	13	67	theme	cytokine	2110:2117	arg1	levels					2119:2124	altered cytokine levels	2102:2124	altered cytokine levels	2102:2124	CONCLUSIONS We demonstrate altered EV production in BV2 microglial cells and altered cytokine levels and protein composition carried by EVs in response to LPS challenge.
29807527	10	68	theme	LPS-activated	1635:1647	arg1	microglia					1649:1657	LPS-activated microglia	1635:1657	LPS-activated microglia	1635:1657	Finally, via mass spectrometry, we identified 49 unique proteins in EVs released from LPS-activated microglia compared to control EVs (58 proteins in EVs released from LPS-activated microglia and 37 from control EVs).
29807527	8	69	from	increase	1340:1347	arg1	levels					1371:1376	the intravesicular levels	1352:1376	the intravesicular levels of TNF and interleukin (IL)-6	1352:1406	RESULTS Our analysis of EVs originating from activated BV2 microglia revealed a significant increase in the intravesicular levels of TNF and interleukin (IL)-6.
29807527	1	70	theme	central	181:187	arg1	system					197:202	the central nervous system	177:202	the central nervous system (CNS)	177:208	BACKGROUND Activated microglia play an essential role in inflammatory responses elicited in the central nervous system (CNS).
29807527	1	70	theme	central	181:187	arg1	CNS					205:207	CNS	205:207	CNS	205:207	BACKGROUND Activated microglia play an essential role in inflammatory responses elicited in the central nervous system (CNS).
29807527	0	71	theme	extracellular	46:58	arg1	vesicles					60:67	extracellular vesicles	46:67	extracellular vesicles from microglia	46:82	Inflammation leads to distinct populations of extracellular vesicles from microglia.
29807527	5	72	theme	EVs	840:842	arg1	alterations					816:826	the alterations	812:826	the alterations of secreted EVs from a microglial cell line (BV2) following lipopolysaccharide (LPS) and tumor necrosis factor (TNF) inhibitor (etanercept) treatments	812:977	METHODS We have utilized western blot, nanoparticle tracking analysis (NTA), and mass spectrometry to characterize EVs and examine the alterations of secreted EVs from a microglial cell line (BV2) following lipopolysaccharide (LPS) and tumor necrosis factor (TNF) inhibitor (etanercept) treatments, or either alone.
29807527	10	73	attach	released	1703:1710	arg2	EVs					1699:1701	EVs	1699:1701	EVs released from LPS-activated microglia	1699:1739	Finally, via mass spectrometry, we identified 49 unique proteins in EVs released from LPS-activated microglia compared to control EVs (58 proteins in EVs released from LPS-activated microglia and 37 from control EVs).
29807527	10	73	attach	released	1703:1710	arg1	microglia					1731:1739	LPS-activated microglia	1717:1739	LPS-activated microglia	1717:1739	Finally, via mass spectrometry, we identified 49 unique proteins in EVs released from LPS-activated microglia compared to control EVs (58 proteins in EVs released from LPS-activated microglia and 37 from control EVs).
29807527	2	74	theme	inflammatory	305:316	arg1	signals					318:324	inflammatory signals	305:324	inflammatory signals	305:324	Microglia-derived extracellular vesicles (EVs) are suggested to be involved in propagation of inflammatory signals and in the modulation of cell-to-cell communication.
29807527	13	75	dep	CONCLUSIONS	2025:2035	arg1	demonstrate					2040:2050	demonstrate	2040:2050	demonstrate altered EV production in BV2 microglial cells and altered cytokine levels and protein composition carried by EVs in response to LPS challenge	2040:2192	CONCLUSIONS We demonstrate altered EV production in BV2 microglial cells and altered cytokine levels and protein composition carried by EVs in response to LPS challenge.
29807527	4	76	theme	inflammation	621:632	arg1	effects					610:616	the effects	606:616	the effects of inflammation on the EV size, quantity, and protein content	606:678	Here, we characterized microglial EVs under inflammatory conditions and investigated the effects of inflammation on the EV size, quantity, and protein content.
29807527	0	77	from	microglia	74:82	arg1	populations					31:41	distinct populations	22:41	distinct populations of extracellular vesicles from microglia	22:82	Inflammation leads to distinct populations of extracellular vesicles from microglia.
29807527	0	77	from	microglia	74:82	arg1	vesicles					60:67	extracellular vesicles	46:67	extracellular vesicles from microglia	46:82	Inflammation leads to distinct populations of extracellular vesicles from microglia.
29807527	5	78	from	alterations	816:826	arg1	BV2					873:875	BV2	873:875	BV2	873:875	METHODS We have utilized western blot, nanoparticle tracking analysis (NTA), and mass spectrometry to characterize EVs and examine the alterations of secreted EVs from a microglial cell line (BV2) following lipopolysaccharide (LPS) and tumor necrosis factor (TNF) inhibitor (etanercept) treatments, or either alone.
29807527	5	78	from	alterations	816:826	arg1	line					867:870	a microglial cell line	849:870	a microglial cell line (BV2) following lipopolysaccharide (LPS) and tumor necrosis factor (TNF) inhibitor (etanercept) treatments	849:977	METHODS We have utilized western blot, nanoparticle tracking analysis (NTA), and mass spectrometry to characterize EVs and examine the alterations of secreted EVs from a microglial cell line (BV2) following lipopolysaccharide (LPS) and tumor necrosis factor (TNF) inhibitor (etanercept) treatments, or either alone.
29807527	1	79	theme	essential	124:132	arg1	role					134:137	an essential role	121:137	an essential role	121:137	BACKGROUND Activated microglia play an essential role in inflammatory responses elicited in the central nervous system (CNS).
29807527	11	80	theme	proteins	1838:1845	arg1	increase					1826:1833	a large increase	1818:1833	a large increase of proteins related to translation and transcription in EVs from LPS	1818:1902	According to Gene Ontology (GO) analysis, we found a large increase of proteins related to translation and transcription in EVs from LPS.
29807527	10	81	theme	control	1671:1677	arg1	EVs					1679:1681	control EVs	1671:1681	control EVs (58 proteins in EVs released from LPS-activated microglia and 37 from control EVs)	1671:1764	Finally, via mass spectrometry, we identified 49 unique proteins in EVs released from LPS-activated microglia compared to control EVs (58 proteins in EVs released from LPS-activated microglia and 37 from control EVs).
29807527	10	82	dep	EVs	1679:1681	arg1	37					1745:1746	37	1745:1746	37	1745:1746	Finally, via mass spectrometry, we identified 49 unique proteins in EVs released from LPS-activated microglia compared to control EVs (58 proteins in EVs released from LPS-activated microglia and 37 from control EVs).
29807527	10	82	dep	EVs	1679:1681	arg1	proteins					1687:1694	58 proteins	1684:1694	58 proteins in EVs released from LPS-activated microglia	1684:1739	Finally, via mass spectrometry, we identified 49 unique proteins in EVs released from LPS-activated microglia compared to control EVs (58 proteins in EVs released from LPS-activated microglia and 37 from control EVs).
29807527	7	83	theme	cerebral	1163:1170	arg1	stroke					1138:1143	experimental stroke	1125:1143	experimental stroke (permanent middle cerebral artery occlusion)	1125:1188	We also subjected TNF knockout mice to experimental stroke (permanent middle cerebral artery occlusion) and validated the effect of TNF inhibition on EV release.
29807527	7	83	theme	cerebral	1163:1170	arg1	occlusion					1179:1187	permanent middle cerebral artery occlusion	1146:1187	permanent middle cerebral artery occlusion	1146:1187	We also subjected TNF knockout mice to experimental stroke (permanent middle cerebral artery occlusion) and validated the effect of TNF inhibition on EV release.
29807527	7	84	theme	permanent	1146:1154	arg1	stroke					1138:1143	experimental stroke	1125:1143	experimental stroke (permanent middle cerebral artery occlusion)	1125:1188	We also subjected TNF knockout mice to experimental stroke (permanent middle cerebral artery occlusion) and validated the effect of TNF inhibition on EV release.
29807527	7	84	theme	permanent	1146:1154	arg1	occlusion					1179:1187	permanent middle cerebral artery occlusion	1146:1187	permanent middle cerebral artery occlusion	1146:1187	We also subjected TNF knockout mice to experimental stroke (permanent middle cerebral artery occlusion) and validated the effect of TNF inhibition on EV release.
29807527	5	85	theme	cell	862:865	arg1	BV2					873:875	BV2	873:875	BV2	873:875	METHODS We have utilized western blot, nanoparticle tracking analysis (NTA), and mass spectrometry to characterize EVs and examine the alterations of secreted EVs from a microglial cell line (BV2) following lipopolysaccharide (LPS) and tumor necrosis factor (TNF) inhibitor (etanercept) treatments, or either alone.
29807527	5	85	theme	cell	862:865	arg1	line					867:870	a microglial cell line	849:870	a microglial cell line (BV2) following lipopolysaccharide (LPS) and tumor necrosis factor (TNF) inhibitor (etanercept) treatments	849:977	METHODS We have utilized western blot, nanoparticle tracking analysis (NTA), and mass spectrometry to characterize EVs and examine the alterations of secreted EVs from a microglial cell line (BV2) following lipopolysaccharide (LPS) and tumor necrosis factor (TNF) inhibitor (etanercept) treatments, or either alone.
29807527	13	86	from	production	2063:2072	arg1	cells					2092:2096	BV2 microglial cells	2077:2096	BV2 microglial cells	2077:2096	CONCLUSIONS We demonstrate altered EV production in BV2 microglial cells and altered cytokine levels and protein composition carried by EVs in response to LPS challenge.
29807527	5	87	theme	western	706:712	arg1	blot					714:717	western blot	706:717	western blot	706:717	METHODS We have utilized western blot, nanoparticle tracking analysis (NTA), and mass spectrometry to characterize EVs and examine the alterations of secreted EVs from a microglial cell line (BV2) following lipopolysaccharide (LPS) and tumor necrosis factor (TNF) inhibitor (etanercept) treatments, or either alone.
29807527	5	88	from	line	867:870	arg1	alterations					816:826	the alterations	812:826	the alterations of secreted EVs from a microglial cell line (BV2) following lipopolysaccharide (LPS) and tumor necrosis factor (TNF) inhibitor (etanercept) treatments	812:977	METHODS We have utilized western blot, nanoparticle tracking analysis (NTA), and mass spectrometry to characterize EVs and examine the alterations of secreted EVs from a microglial cell line (BV2) following lipopolysaccharide (LPS) and tumor necrosis factor (TNF) inhibitor (etanercept) treatments, or either alone.
29807527	5	88	from	line	867:870	arg1	EVs					840:842	secreted EVs	831:842	secreted EVs from a microglial cell line (BV2) following lipopolysaccharide (LPS) and tumor necrosis factor (TNF) inhibitor (etanercept) treatments	831:977	METHODS We have utilized western blot, nanoparticle tracking analysis (NTA), and mass spectrometry to characterize EVs and examine the alterations of secreted EVs from a microglial cell line (BV2) following lipopolysaccharide (LPS) and tumor necrosis factor (TNF) inhibitor (etanercept) treatments, or either alone.
29807527	5	89	theme	necrosis	923:930	arg1	inhibitor					945:953	tumor necrosis factor (TNF) inhibitor	917:953	tumor necrosis factor (TNF) inhibitor	917:953	METHODS We have utilized western blot, nanoparticle tracking analysis (NTA), and mass spectrometry to characterize EVs and examine the alterations of secreted EVs from a microglial cell line (BV2) following lipopolysaccharide (LPS) and tumor necrosis factor (TNF) inhibitor (etanercept) treatments, or either alone.
29807527	3	90	theme	EVs	438:440	arg1	regulation					424:433	the regulation	420:433	the regulation of EVs	420:440	However, there is a lack of knowledge on the regulation of EVs and how this in turn facilitates the communication between cells in the brain.
29807527	7	91	theme	knockout	1108:1115	arg1	mice					1117:1120	TNF knockout mice	1104:1120	TNF knockout mice	1104:1120	We also subjected TNF knockout mice to experimental stroke (permanent middle cerebral artery occlusion) and validated the effect of TNF inhibition on EV release.
29807527	12	92	located	found	1959:1963	arg2	proteins					1950:1957	proteins	1950:1957	proteins found in EVs released from LPS treated cells compared to control	1950:2022	Importantly, we showed a distinct profile of proteins found in EVs released from LPS treated cells compared to control.
29807527	12	92	located	found	1959:1963	arg1	EVs					1968:1970	EVs	1968:1970	EVs released from LPS treated cells compared to control	1968:2022	Importantly, we showed a distinct profile of proteins found in EVs released from LPS treated cells compared to control.
29807527	5	93	theme	lipopolysaccharide	888:905	arg1	treatments					968:977	lipopolysaccharide (LPS) and tumor necrosis factor (TNF) inhibitor (etanercept) treatments	888:977	lipopolysaccharide (LPS) and tumor necrosis factor (TNF) inhibitor (etanercept) treatments	888:977	METHODS We have utilized western blot, nanoparticle tracking analysis (NTA), and mass spectrometry to characterize EVs and examine the alterations of secreted EVs from a microglial cell line (BV2) following lipopolysaccharide (LPS) and tumor necrosis factor (TNF) inhibitor (etanercept) treatments, or either alone.
29807527	8	94	theme	activated	1293:1301	arg1	microglia					1307:1315	activated BV2 microglia	1293:1315	activated BV2 microglia	1293:1315	RESULTS Our analysis of EVs originating from activated BV2 microglia revealed a significant increase in the intravesicular levels of TNF and interleukin (IL)-6.
29807527	11	95	theme	Gene	1780:1783	arg1	Ontology					1785:1792	Gene Ontology	1780:1792	Gene Ontology (GO) analysis	1780:1806	According to Gene Ontology (GO) analysis, we found a large increase of proteins related to translation and transcription in EVs from LPS.
29807527	0	96	theme	distinct	22:29	arg1	populations					31:41	distinct populations	22:41	distinct populations of extracellular vesicles from microglia	22:82	Inflammation leads to distinct populations of extracellular vesicles from microglia.
29807527	13	97	theme	BV2	2077:2079	arg1	cells					2092:2096	BV2 microglial cells	2077:2096	BV2 microglial cells	2077:2096	CONCLUSIONS We demonstrate altered EV production in BV2 microglial cells and altered cytokine levels and protein composition carried by EVs in response to LPS challenge.
29807527	7	98	theme	EV	1236:1237	arg1	release					1239:1245	EV release	1236:1245	EV release	1236:1245	We also subjected TNF knockout mice to experimental stroke (permanent middle cerebral artery occlusion) and validated the effect of TNF inhibition on EV release.
29807527	10	99	attach	released	1621:1628	arg1	microglia					1649:1657	LPS-activated microglia	1635:1657	LPS-activated microglia	1635:1657	Finally, via mass spectrometry, we identified 49 unique proteins in EVs released from LPS-activated microglia compared to control EVs (58 proteins in EVs released from LPS-activated microglia and 37 from control EVs).
29807527	10	99	attach	released	1621:1628	arg2	EVs					1617:1619	EVs	1617:1619	EVs released from LPS-activated microglia	1617:1657	Finally, via mass spectrometry, we identified 49 unique proteins in EVs released from LPS-activated microglia compared to control EVs (58 proteins in EVs released from LPS-activated microglia and 37 from control EVs).
29807527	10	100	theme	control	1753:1759	arg1	EVs					1761:1763	control EVs	1753:1763	control EVs	1753:1763	Finally, via mass spectrometry, we identified 49 unique proteins in EVs released from LPS-activated microglia compared to control EVs (58 proteins in EVs released from LPS-activated microglia and 37 from control EVs).
29807527	13	101	theme	LPS	2180:2182	arg1	challenge					2184:2192	LPS challenge	2180:2192	LPS challenge	2180:2192	CONCLUSIONS We demonstrate altered EV production in BV2 microglial cells and altered cytokine levels and protein composition carried by EVs in response to LPS challenge.
29807527	7	102	theme	inhibition	1222:1231	arg1	effect					1208:1213	the effect	1204:1213	the effect of TNF inhibition on EV release	1204:1245	We also subjected TNF knockout mice to experimental stroke (permanent middle cerebral artery occlusion) and validated the effect of TNF inhibition on EV release.
29807527	7	103	theme	experimental	1125:1136	arg1	stroke					1138:1143	experimental stroke	1125:1143	experimental stroke (permanent middle cerebral artery occlusion)	1125:1188	We also subjected TNF knockout mice to experimental stroke (permanent middle cerebral artery occlusion) and validated the effect of TNF inhibition on EV release.
29807527	7	103	theme	experimental	1125:1136	arg1	occlusion					1179:1187	permanent middle cerebral artery occlusion	1146:1187	permanent middle cerebral artery occlusion	1146:1187	We also subjected TNF knockout mice to experimental stroke (permanent middle cerebral artery occlusion) and validated the effect of TNF inhibition on EV release.
29807527	0	104	theme	vesicles	60:67	arg1	populations					31:41	distinct populations	22:41	distinct populations of extracellular vesicles from microglia	22:82	Inflammation leads to distinct populations of extracellular vesicles from microglia.
29807527	13	105	theme	altered	2052:2058	arg1	production					2063:2072	altered EV production	2052:2072	altered EV production in BV2 microglial cells	2052:2096	CONCLUSIONS We demonstrate altered EV production in BV2 microglial cells and altered cytokine levels and protein composition carried by EVs in response to LPS challenge.
29807527	8	106	dep	RESULTS	1248:1254	arg1	revealed					1317:1324	revealed	1317:1324	revealed a significant increase in the intravesicular levels of TNF and interleukin (IL)-6	1317:1406	RESULTS Our analysis of EVs originating from activated BV2 microglia revealed a significant increase in the intravesicular levels of TNF and interleukin (IL)-6.
29807527	6	107	theme	western	1072:1078	arg1	blot					1080:1083	western blot	1072:1083	western blot	1072:1083	The inflammatory responses were measured with multiplex cytokine ELISA and western blot.
29807527	2	108	theme	Microglia-derived	211:227	arg1	EVs					253:255	EVs	253:255	EVs	253:255	Microglia-derived extracellular vesicles (EVs) are suggested to be involved in propagation of inflammatory signals and in the modulation of cell-to-cell communication.
29807527	2	108	theme	Microglia-derived	211:227	arg1	vesicles					243:250	Microglia-derived extracellular vesicles	211:250	Microglia-derived extracellular vesicles (EVs)	211:256	Microglia-derived extracellular vesicles (EVs) are suggested to be involved in propagation of inflammatory signals and in the modulation of cell-to-cell communication.
29807527	5	109	theme	inhibitor	945:953	arg1	treatments					968:977	lipopolysaccharide (LPS) and tumor necrosis factor (TNF) inhibitor (etanercept) treatments	888:977	lipopolysaccharide (LPS) and tumor necrosis factor (TNF) inhibitor (etanercept) treatments	888:977	METHODS We have utilized western blot, nanoparticle tracking analysis (NTA), and mass spectrometry to characterize EVs and examine the alterations of secreted EVs from a microglial cell line (BV2) following lipopolysaccharide (LPS) and tumor necrosis factor (TNF) inhibitor (etanercept) treatments, or either alone.
29807527	5	110	theme	factor	932:937	arg1	inhibitor					945:953	tumor necrosis factor (TNF) inhibitor	917:953	tumor necrosis factor (TNF) inhibitor	917:953	METHODS We have utilized western blot, nanoparticle tracking analysis (NTA), and mass spectrometry to characterize EVs and examine the alterations of secreted EVs from a microglial cell line (BV2) following lipopolysaccharide (LPS) and tumor necrosis factor (TNF) inhibitor (etanercept) treatments, or either alone.
29807527	12	111	theme	treated	1990:1996	arg1	cells					1998:2002	LPS treated cells	1986:2002	LPS treated cells	1986:2002	Importantly, we showed a distinct profile of proteins found in EVs released from LPS treated cells compared to control.
29807527	6	112	theme	multiplex	1043:1051	arg1	ELISA					1062:1066	multiplex cytokine ELISA	1043:1066	multiplex cytokine ELISA	1043:1066	The inflammatory responses were measured with multiplex cytokine ELISA and western blot.
29807527	11	113	from	LPS	1900:1902	arg1	EVs					1891:1893	EVs	1891:1893	EVs from LPS	1891:1902	According to Gene Ontology (GO) analysis, we found a large increase of proteins related to translation and transcription in EVs from LPS.
29807527	11	113	from	LPS	1900:1902	arg1	transcription					1874:1886	transcription	1874:1886	transcription	1874:1886	According to Gene Ontology (GO) analysis, we found a large increase of proteins related to translation and transcription in EVs from LPS.
29807527	11	113	from	LPS	1900:1902	arg1	translation					1858:1868	translation	1858:1868	translation	1858:1868	According to Gene Ontology (GO) analysis, we found a large increase of proteins related to translation and transcription in EVs from LPS.
30088083	8	0	theme	higher	1133:1138	arg1	response					1140:1147	a higher response	1131:1147	a higher response to different cultivating pHs	1131:1176	Interestingly, pH excursions led to significantly higher galactosylation for one cell line, which also had a higher response to different cultivating pHs.
30088083	9	1	from	impact	1228:1233	arg1	galactosylation					1255:1269	galactosylation	1255:1269	galactosylation for the other two cell lines, both of which also had minimal response to cultivating pH. This suggests that the impact of pH excursions on antibody N-linked glycosylation is cell line specific and is closely related to cell line response to cultivating pH	1255:1525	On the other hand, there was no such substantial impact of pH excursions on galactosylation for the other two cell lines, both of which also had minimal response to cultivating pH. This suggests that the impact of pH excursions on antibody N-linked glycosylation is cell line specific and is closely related to cell line response to cultivating pH.
30088083	4	2	theme	small-scale	572:582	arg1	bioreactors					584:594	small-scale bioreactors	572:594	small-scale bioreactors	572:594	Frequent pH excursions were introduced by bolus base addition (in total 2-6% of initial volume, fixed bolus addition distributed from day 2 to 8) into small-scale bioreactors.
30088083	6	3	theme	glucose	797:803	arg1	rates					827:831	higher glucose and lactate metabolic rates	790:831	higher glucose and lactate metabolic rates than continuous addition	790:856	Lactate production increase was mainly caused by increased culture pH due to base addition, and bolus addition led to higher glucose and lactate metabolic rates than continuous addition.
30088083	4	4	theme	Frequent	421:428	arg1	excursions					433:442	Frequent pH excursions	421:442	Frequent pH excursions	421:442	Frequent pH excursions were introduced by bolus base addition (in total 2-6% of initial volume, fixed bolus addition distributed from day 2 to 8) into small-scale bioreactors.
30088083	9	5	theme	cultivating	1512:1522	arg1	pH					1524:1525	cultivating pH	1512:1525	cultivating pH	1512:1525	On the other hand, there was no such substantial impact of pH excursions on galactosylation for the other two cell lines, both of which also had minimal response to cultivating pH. This suggests that the impact of pH excursions on antibody N-linked glycosylation is cell line specific and is closely related to cell line response to cultivating pH.
30088083	5	6	theme	lactate	652:658	arg1	production					660:669	lactate production	652:669	lactate production	652:669	Base addition led to increase in osmolality, pCO2, and lactate production.
30088083	8	7	theme	cultivating	1162:1172	arg1	pHs					1174:1176	different cultivating pHs	1152:1176	different cultivating pHs	1152:1176	Interestingly, pH excursions led to significantly higher galactosylation for one cell line, which also had a higher response to different cultivating pHs.
30088083	9	8	theme	other	1186:1190	arg1	hand					1192:1195	the other hand	1182:1195	the other hand	1182:1195	On the other hand, there was no such substantial impact of pH excursions on galactosylation for the other two cell lines, both of which also had minimal response to cultivating pH. This suggests that the impact of pH excursions on antibody N-linked glycosylation is cell line specific and is closely related to cell line response to cultivating pH.
30088083	9	9	theme	pH	1393:1394	arg1	excursions					1396:1405	pH excursions	1393:1405	pH excursions	1393:1405	On the other hand, there was no such substantial impact of pH excursions on galactosylation for the other two cell lines, both of which also had minimal response to cultivating pH. This suggests that the impact of pH excursions on antibody N-linked glycosylation is cell line specific and is closely related to cell line response to cultivating pH.
30088083	3	10	link	N-linked	340:347	arg1	glycosylation					349:361	N-linked glycosylation	340:361	N-linked glycosylation	340:361	Here we investigated the impact of pH excursions on cell culture performance and N-linked glycosylation for three MAb-producing Chinese hamster ovary cell lines.
30088083	3	11	theme	pH	294:295	arg1	excursions					297:306	pH excursions	294:306	pH excursions	294:306	Here we investigated the impact of pH excursions on cell culture performance and N-linked glycosylation for three MAb-producing Chinese hamster ovary cell lines.
30088083	7	12	from	decrease	984:991	arg1	productivity					1010:1021	cell-specific productivity	996:1021	cell-specific productivity	996:1021	For the three cell lines studied, antibody galactosylation increased with the increase in cultivating pH, correlating to the decrease in cell-specific productivity.
30088083	8	13	contain	had	1127:1129	arg1	line					1110:1113	one cell line	1101:1113	one cell line	1101:1113	Interestingly, pH excursions led to significantly higher galactosylation for one cell line, which also had a higher response to different cultivating pHs.
30088083	8	13	contain	had	1127:1129	arg2	response					1140:1147	a higher response	1131:1147	a higher response to different cultivating pHs	1131:1176	Interestingly, pH excursions led to significantly higher galactosylation for one cell line, which also had a higher response to different cultivating pHs.
30088083	9	14	theme	N-linked	1419:1426	arg1	glycosylation					1428:1440	antibody N-linked glycosylation	1410:1440	antibody N-linked glycosylation	1410:1440	On the other hand, there was no such substantial impact of pH excursions on galactosylation for the other two cell lines, both of which also had minimal response to cultivating pH. This suggests that the impact of pH excursions on antibody N-linked glycosylation is cell line specific and is closely related to cell line response to cultivating pH.
30088083	9	15	theme	such	1211:1214	arg1	impact					1228:1233	no such substantial impact	1208:1233	no such substantial impact of pH excursions on galactosylation for the other two cell lines, both of which also had minimal response to cultivating pH. This suggests that the impact of pH excursions on antibody N-linked glycosylation is cell line specific and is closely related to cell line response to cultivating pH	1208:1525	On the other hand, there was no such substantial impact of pH excursions on galactosylation for the other two cell lines, both of which also had minimal response to cultivating pH. This suggests that the impact of pH excursions on antibody N-linked glycosylation is cell line specific and is closely related to cell line response to cultivating pH.
30088083	7	16	theme	cell-specific	996:1008	arg1	productivity					1010:1021	cell-specific productivity	996:1021	cell-specific productivity	996:1021	For the three cell lines studied, antibody galactosylation increased with the increase in cultivating pH, correlating to the decrease in cell-specific productivity.
30088083	3	17	theme	culture	316:322	arg1	performance					324:334	cell culture performance	311:334	cell culture performance	311:334	Here we investigated the impact of pH excursions on cell culture performance and N-linked glycosylation for three MAb-producing Chinese hamster ovary cell lines.
30088083	2	18	theme	suboptimal	227:236	arg1	performance					246:256	suboptimal culture performance	227:256	suboptimal culture performance	227:256	Such excursions could lead to suboptimal culture performance.
30088083	1	19	theme	frequent	114:121	arg1	addition					128:135	frequent base addition	114:135	frequent base addition	114:135	pH excursions exist due to frequent base addition and environmental heterogeneity in large-scale bioreactors.
30088083	9	20	theme	pH	1238:1239	arg1	excursions					1241:1250	pH excursions	1238:1250	pH excursions	1238:1250	On the other hand, there was no such substantial impact of pH excursions on galactosylation for the other two cell lines, both of which also had minimal response to cultivating pH. This suggests that the impact of pH excursions on antibody N-linked glycosylation is cell line specific and is closely related to cell line response to cultivating pH.
30088083	4	21	theme	bolus	463:467	arg1	addition					474:481	bolus base addition	463:481	bolus base addition	463:481	Frequent pH excursions were introduced by bolus base addition (in total 2-6% of initial volume, fixed bolus addition distributed from day 2 to 8) into small-scale bioreactors.
30088083	7	22	from	increase	937:944	arg1	pH					961:962	cultivating pH	949:962	cultivating pH	949:962	For the three cell lines studied, antibody galactosylation increased with the increase in cultivating pH, correlating to the decrease in cell-specific productivity.
30088083	0	23	theme	antibody	54:61	arg1	glycosylation					72:84	antibody N-linked glycosylation	54:84	antibody N-linked glycosylation	54:84	pH excursions impact CHO cell culture performance and antibody N-linked glycosylation.
30088083	4	24	dep	day	555:557	arg1	to					561:562	to	561:562	to	561:562	Frequent pH excursions were introduced by bolus base addition (in total 2-6% of initial volume, fixed bolus addition distributed from day 2 to 8) into small-scale bioreactors.
30088083	1	25	theme	environmental	141:153	arg1	heterogeneity					155:167	environmental heterogeneity	141:167	environmental heterogeneity	141:167	pH excursions exist due to frequent base addition and environmental heterogeneity in large-scale bioreactors.
30088083	4	26	theme	volume	509:514	arg1	%					496:496	total 2-6%	487:496	total 2-6% of initial volume	487:514	Frequent pH excursions were introduced by bolus base addition (in total 2-6% of initial volume, fixed bolus addition distributed from day 2 to 8) into small-scale bioreactors.
30088083	4	26	theme	volume	509:514	arg1	volume					509:514	initial volume	501:514	initial volume	501:514	Frequent pH excursions were introduced by bolus base addition (in total 2-6% of initial volume, fixed bolus addition distributed from day 2 to 8) into small-scale bioreactors.
30088083	8	27	theme	higher	1074:1079	arg1	galactosylation					1081:1095	significantly higher galactosylation	1060:1095	significantly higher galactosylation for one cell line, which also had a higher response to different cultivating pHs	1060:1176	Interestingly, pH excursions led to significantly higher galactosylation for one cell line, which also had a higher response to different cultivating pHs.
30088083	1	28	from	heterogeneity	155:167	arg1	bioreactors					184:194	large-scale bioreactors	172:194	large-scale bioreactors	172:194	pH excursions exist due to frequent base addition and environmental heterogeneity in large-scale bioreactors.
30088083	3	29	theme	Chinese	387:393	arg1	lines					414:418	three MAb-producing Chinese hamster ovary cell lines	367:418	three MAb-producing Chinese hamster ovary cell lines	367:418	Here we investigated the impact of pH excursions on cell culture performance and N-linked glycosylation for three MAb-producing Chinese hamster ovary cell lines.
30088083	4	30	theme	fixed	517:521	arg1	addition					529:536	fixed bolus addition	517:536	fixed bolus addition	517:536	Frequent pH excursions were introduced by bolus base addition (in total 2-6% of initial volume, fixed bolus addition distributed from day 2 to 8) into small-scale bioreactors.
30088083	9	31	theme	other	1279:1283	arg1	lines					1294:1298	the other two cell lines	1275:1298	the other two cell lines	1275:1298	On the other hand, there was no such substantial impact of pH excursions on galactosylation for the other two cell lines, both of which also had minimal response to cultivating pH. This suggests that the impact of pH excursions on antibody N-linked glycosylation is cell line specific and is closely related to cell line response to cultivating pH.
30088083	9	32	link	N-linked	1419:1426	arg1	glycosylation					1428:1440	antibody N-linked glycosylation	1410:1440	antibody N-linked glycosylation	1410:1440	On the other hand, there was no such substantial impact of pH excursions on galactosylation for the other two cell lines, both of which also had minimal response to cultivating pH. This suggests that the impact of pH excursions on antibody N-linked glycosylation is cell line specific and is closely related to cell line response to cultivating pH.
30088083	9	33	theme	line	1495:1498	arg1	response					1500:1507	cell line response	1490:1507	cell line response to cultivating pH	1490:1525	On the other hand, there was no such substantial impact of pH excursions on galactosylation for the other two cell lines, both of which also had minimal response to cultivating pH. This suggests that the impact of pH excursions on antibody N-linked glycosylation is cell line specific and is closely related to cell line response to cultivating pH.
30088083	9	34	theme	cell	1289:1292	arg1	lines					1294:1298	the other two cell lines	1275:1298	the other two cell lines	1275:1298	On the other hand, there was no such substantial impact of pH excursions on galactosylation for the other two cell lines, both of which also had minimal response to cultivating pH. This suggests that the impact of pH excursions on antibody N-linked glycosylation is cell line specific and is closely related to cell line response to cultivating pH.
30088083	3	35	theme	ovary	403:407	arg1	lines					414:418	three MAb-producing Chinese hamster ovary cell lines	367:418	three MAb-producing Chinese hamster ovary cell lines	367:418	Here we investigated the impact of pH excursions on cell culture performance and N-linked glycosylation for three MAb-producing Chinese hamster ovary cell lines.
30088083	6	36	theme	Lactate	672:678	arg1	production					680:689	Lactate production	672:689	Lactate production increase	672:698	Lactate production increase was mainly caused by increased culture pH due to base addition, and bolus addition led to higher glucose and lactate metabolic rates than continuous addition.
30088083	0	37	theme	impact	14:19	arg1	performance					38:48	impact CHO cell culture performance	14:48	impact CHO cell culture performance	14:48	pH excursions impact CHO cell culture performance and antibody N-linked glycosylation.
30088083	5	38	from	increase	618:625	arg1	osmolality					630:639	osmolality	630:639	osmolality	630:639	Base addition led to increase in osmolality, pCO2, and lactate production.
30088083	5	38	from	increase	618:625	arg1	production					660:669	lactate production	652:669	lactate production	652:669	Base addition led to increase in osmolality, pCO2, and lactate production.
30088083	5	38	from	increase	618:625	arg1	pCO2					642:645	pCO2	642:645	pCO2	642:645	Base addition led to increase in osmolality, pCO2, and lactate production.
30088083	9	39	from	impact	1383:1388	arg1	glycosylation					1428:1440	antibody N-linked glycosylation	1410:1440	antibody N-linked glycosylation	1410:1440	On the other hand, there was no such substantial impact of pH excursions on galactosylation for the other two cell lines, both of which also had minimal response to cultivating pH. This suggests that the impact of pH excursions on antibody N-linked glycosylation is cell line specific and is closely related to cell line response to cultivating pH.
30088083	0	40	theme	cell	25:28	arg1	performance					38:48	impact CHO cell culture performance	14:48	impact CHO cell culture performance	14:48	pH excursions impact CHO cell culture performance and antibody N-linked glycosylation.
30088083	6	41	theme	continuous	838:847	arg1	addition					849:856	continuous addition	838:856	continuous addition	838:856	Lactate production increase was mainly caused by increased culture pH due to base addition, and bolus addition led to higher glucose and lactate metabolic rates than continuous addition.
30088083	6	42	theme	base	749:752	arg1	addition					754:761	base addition	749:761	base addition	749:761	Lactate production increase was mainly caused by increased culture pH due to base addition, and bolus addition led to higher glucose and lactate metabolic rates than continuous addition.
30088083	9	43	theme	minimal	1324:1330	arg1	response					1332:1339	minimal response	1324:1339	minimal response to cultivating pH. This suggests that the impact of pH excursions on antibody N-linked glycosylation is cell line specific and is closely related to cell line response to cultivating pH	1324:1525	On the other hand, there was no such substantial impact of pH excursions on galactosylation for the other two cell lines, both of which also had minimal response to cultivating pH. This suggests that the impact of pH excursions on antibody N-linked glycosylation is cell line specific and is closely related to cell line response to cultivating pH.
30088083	8	44	theme	cell	1105:1108	arg1	line					1110:1113	one cell line	1101:1113	one cell line	1101:1113	Interestingly, pH excursions led to significantly higher galactosylation for one cell line, which also had a higher response to different cultivating pHs.
30088083	0	45	link	N-linked	63:70	arg1	glycosylation					72:84	antibody N-linked glycosylation	54:84	antibody N-linked glycosylation	54:84	pH excursions impact CHO cell culture performance and antibody N-linked glycosylation.
30088083	7	46	theme	antibody	893:900	arg1	galactosylation					902:916	antibody galactosylation	893:916	antibody galactosylation	893:916	For the three cell lines studied, antibody galactosylation increased with the increase in cultivating pH, correlating to the decrease in cell-specific productivity.
30088083	6	47	theme	lactate	809:815	arg1	rates					827:831	higher glucose and lactate metabolic rates	790:831	higher glucose and lactate metabolic rates than continuous addition	790:856	Lactate production increase was mainly caused by increased culture pH due to base addition, and bolus addition led to higher glucose and lactate metabolic rates than continuous addition.
30088083	6	48	theme	culture	731:737	arg1	pH					739:740	increased culture pH	721:740	increased culture pH due to base addition	721:761	Lactate production increase was mainly caused by increased culture pH due to base addition, and bolus addition led to higher glucose and lactate metabolic rates than continuous addition.
30088083	7	49	theme	cell	873:876	arg1	lines					878:882	the three cell lines	863:882	the three cell lines studied	863:890	For the three cell lines studied, antibody galactosylation increased with the increase in cultivating pH, correlating to the decrease in cell-specific productivity.
30088083	3	50	from	impact	284:289	arg1	glycosylation					349:361	N-linked glycosylation	340:361	N-linked glycosylation	340:361	Here we investigated the impact of pH excursions on cell culture performance and N-linked glycosylation for three MAb-producing Chinese hamster ovary cell lines.
30088083	3	50	from	impact	284:289	arg1	performance					324:334	cell culture performance	311:334	cell culture performance	311:334	Here we investigated the impact of pH excursions on cell culture performance and N-linked glycosylation for three MAb-producing Chinese hamster ovary cell lines.
30088083	4	51	theme	pH	430:431	arg1	excursions					433:442	Frequent pH excursions	421:442	Frequent pH excursions	421:442	Frequent pH excursions were introduced by bolus base addition (in total 2-6% of initial volume, fixed bolus addition distributed from day 2 to 8) into small-scale bioreactors.
30088083	6	52	theme	higher	790:795	arg1	rates					827:831	higher glucose and lactate metabolic rates	790:831	higher glucose and lactate metabolic rates than continuous addition	790:856	Lactate production increase was mainly caused by increased culture pH due to base addition, and bolus addition led to higher glucose and lactate metabolic rates than continuous addition.
30088083	1	53	theme	large-scale	172:182	arg1	bioreactors					184:194	large-scale bioreactors	172:194	large-scale bioreactors	172:194	pH excursions exist due to frequent base addition and environmental heterogeneity in large-scale bioreactors.
30088083	8	54	theme	different	1152:1160	arg1	pHs					1174:1176	different cultivating pHs	1152:1176	different cultivating pHs	1152:1176	Interestingly, pH excursions led to significantly higher galactosylation for one cell line, which also had a higher response to different cultivating pHs.
30088083	6	55	theme	bolus	768:772	arg1	addition					774:781	bolus addition	768:781	bolus addition	768:781	Lactate production increase was mainly caused by increased culture pH due to base addition, and bolus addition led to higher glucose and lactate metabolic rates than continuous addition.
30088083	9	56	theme	excursions	1396:1405	arg1	specific					1455:1462	specific	1455:1462	specific	1455:1462	On the other hand, there was no such substantial impact of pH excursions on galactosylation for the other two cell lines, both of which also had minimal response to cultivating pH. This suggests that the impact of pH excursions on antibody N-linked glycosylation is cell line specific and is closely related to cell line response to cultivating pH.
30088083	9	56	theme	excursions	1396:1405	arg1	related					1479:1485	related	1479:1485	related	1479:1485	On the other hand, there was no such substantial impact of pH excursions on galactosylation for the other two cell lines, both of which also had minimal response to cultivating pH. This suggests that the impact of pH excursions on antibody N-linked glycosylation is cell line specific and is closely related to cell line response to cultivating pH.
30088083	9	56	theme	excursions	1396:1405	arg1	impact					1383:1388	the impact	1379:1388	the impact of pH excursions on antibody N-linked glycosylation	1379:1440	On the other hand, there was no such substantial impact of pH excursions on galactosylation for the other two cell lines, both of which also had minimal response to cultivating pH. This suggests that the impact of pH excursions on antibody N-linked glycosylation is cell line specific and is closely related to cell line response to cultivating pH.
30088083	5	57	theme	Base	597:600	arg1	addition					602:609	Base addition	597:609	Base addition	597:609	Base addition led to increase in osmolality, pCO2, and lactate production.
30088083	9	58	theme	antibody	1410:1417	arg1	glycosylation					1428:1440	antibody N-linked glycosylation	1410:1440	antibody N-linked glycosylation	1410:1440	On the other hand, there was no such substantial impact of pH excursions on galactosylation for the other two cell lines, both of which also had minimal response to cultivating pH. This suggests that the impact of pH excursions on antibody N-linked glycosylation is cell line specific and is closely related to cell line response to cultivating pH.
30088083	3	59	theme	excursions	297:306	arg1	impact					284:289	the impact	280:289	the impact of pH excursions on cell culture performance and N-linked glycosylation for three MAb-producing Chinese hamster ovary cell lines	280:418	Here we investigated the impact of pH excursions on cell culture performance and N-linked glycosylation for three MAb-producing Chinese hamster ovary cell lines.
30088083	4	60	theme	total	487:491	arg1	%					496:496	total 2-6%	487:496	total 2-6% of initial volume	487:514	Frequent pH excursions were introduced by bolus base addition (in total 2-6% of initial volume, fixed bolus addition distributed from day 2 to 8) into small-scale bioreactors.
30088083	4	60	theme	total	487:491	arg1	volume					509:514	initial volume	501:514	initial volume	501:514	Frequent pH excursions were introduced by bolus base addition (in total 2-6% of initial volume, fixed bolus addition distributed from day 2 to 8) into small-scale bioreactors.
30088083	9	61	theme	substantial	1216:1226	arg1	impact					1228:1233	no such substantial impact	1208:1233	no such substantial impact of pH excursions on galactosylation for the other two cell lines, both of which also had minimal response to cultivating pH. This suggests that the impact of pH excursions on antibody N-linked glycosylation is cell line specific and is closely related to cell line response to cultivating pH	1208:1525	On the other hand, there was no such substantial impact of pH excursions on galactosylation for the other two cell lines, both of which also had minimal response to cultivating pH. This suggests that the impact of pH excursions on antibody N-linked glycosylation is cell line specific and is closely related to cell line response to cultivating pH.
30088083	2	62	theme	culture	238:244	arg1	performance					246:256	suboptimal culture performance	227:256	suboptimal culture performance	227:256	Such excursions could lead to suboptimal culture performance.
30088083	1	63	theme	base	123:126	arg1	addition					128:135	frequent base addition	114:135	frequent base addition	114:135	pH excursions exist due to frequent base addition and environmental heterogeneity in large-scale bioreactors.
30088083	3	64	theme	cell	311:314	arg1	performance					324:334	cell culture performance	311:334	cell culture performance	311:334	Here we investigated the impact of pH excursions on cell culture performance and N-linked glycosylation for three MAb-producing Chinese hamster ovary cell lines.
30088083	4	65	theme	base	469:472	arg1	addition					474:481	bolus base addition	463:481	bolus base addition	463:481	Frequent pH excursions were introduced by bolus base addition (in total 2-6% of initial volume, fixed bolus addition distributed from day 2 to 8) into small-scale bioreactors.
30088083	0	66	theme	N-linked	63:70	arg1	glycosylation					72:84	antibody N-linked glycosylation	54:84	antibody N-linked glycosylation	54:84	pH excursions impact CHO cell culture performance and antibody N-linked glycosylation.
30088083	9	67	theme	excursions	1241:1250	arg1	impact					1228:1233	no such substantial impact	1208:1233	no such substantial impact of pH excursions on galactosylation for the other two cell lines, both of which also had minimal response to cultivating pH. This suggests that the impact of pH excursions on antibody N-linked glycosylation is cell line specific and is closely related to cell line response to cultivating pH	1208:1525	On the other hand, there was no such substantial impact of pH excursions on galactosylation for the other two cell lines, both of which also had minimal response to cultivating pH. This suggests that the impact of pH excursions on antibody N-linked glycosylation is cell line specific and is closely related to cell line response to cultivating pH.
30088083	7	68	theme	cultivating	949:959	arg1	pH					961:962	cultivating pH	949:962	cultivating pH	949:962	For the three cell lines studied, antibody galactosylation increased with the increase in cultivating pH, correlating to the decrease in cell-specific productivity.
30088083	3	69	theme	N-linked	340:347	arg1	glycosylation					349:361	N-linked glycosylation	340:361	N-linked glycosylation	340:361	Here we investigated the impact of pH excursions on cell culture performance and N-linked glycosylation for three MAb-producing Chinese hamster ovary cell lines.
30088083	4	70	theme	bolus	523:527	arg1	addition					529:536	fixed bolus addition	517:536	fixed bolus addition	517:536	Frequent pH excursions were introduced by bolus base addition (in total 2-6% of initial volume, fixed bolus addition distributed from day 2 to 8) into small-scale bioreactors.
30088083	8	71	theme	pH	1039:1040	arg1	excursions					1042:1051	pH excursions	1039:1051	pH excursions	1039:1051	Interestingly, pH excursions led to significantly higher galactosylation for one cell line, which also had a higher response to different cultivating pHs.
30088083	9	72	theme	cell	1490:1493	arg1	response					1500:1507	cell line response	1490:1507	cell line response to cultivating pH	1490:1525	On the other hand, there was no such substantial impact of pH excursions on galactosylation for the other two cell lines, both of which also had minimal response to cultivating pH. This suggests that the impact of pH excursions on antibody N-linked glycosylation is cell line specific and is closely related to cell line response to cultivating pH.
30088083	3	73	theme	hamster	395:401	arg1	lines					414:418	three MAb-producing Chinese hamster ovary cell lines	367:418	three MAb-producing Chinese hamster ovary cell lines	367:418	Here we investigated the impact of pH excursions on cell culture performance and N-linked glycosylation for three MAb-producing Chinese hamster ovary cell lines.
30088083	3	74	theme	MAb-producing	373:385	arg1	lines					414:418	three MAb-producing Chinese hamster ovary cell lines	367:418	three MAb-producing Chinese hamster ovary cell lines	367:418	Here we investigated the impact of pH excursions on cell culture performance and N-linked glycosylation for three MAb-producing Chinese hamster ovary cell lines.
30088083	4	75	theme	initial	501:507	arg1	volume					509:514	initial volume	501:514	initial volume	501:514	Frequent pH excursions were introduced by bolus base addition (in total 2-6% of initial volume, fixed bolus addition distributed from day 2 to 8) into small-scale bioreactors.
30088083	1	76	from	addition	128:135	arg1	bioreactors					184:194	large-scale bioreactors	172:194	large-scale bioreactors	172:194	pH excursions exist due to frequent base addition and environmental heterogeneity in large-scale bioreactors.
30088083	1	77	theme	pH	87:88	arg1	excursions					90:99	pH excursions	87:99	pH excursions	87:99	pH excursions exist due to frequent base addition and environmental heterogeneity in large-scale bioreactors.
30088083	6	78	theme	production	680:689	arg1	increase					691:698	Lactate production increase	672:698	Lactate production increase	672:698	Lactate production increase was mainly caused by increased culture pH due to base addition, and bolus addition led to higher glucose and lactate metabolic rates than continuous addition.
30088083	0	79	theme	CHO	21:23	arg1	performance					38:48	impact CHO cell culture performance	14:48	impact CHO cell culture performance	14:48	pH excursions impact CHO cell culture performance and antibody N-linked glycosylation.
30088083	3	80	theme	cell	409:412	arg1	lines					414:418	three MAb-producing Chinese hamster ovary cell lines	367:418	three MAb-producing Chinese hamster ovary cell lines	367:418	Here we investigated the impact of pH excursions on cell culture performance and N-linked glycosylation for three MAb-producing Chinese hamster ovary cell lines.
30088083	2	81	theme	Such	197:200	arg1	excursions					202:211	Such excursions	197:211	Such excursions	197:211	Such excursions could lead to suboptimal culture performance.
30088083	0	82	theme	culture	30:36	arg1	performance					38:48	impact CHO cell culture performance	14:48	impact CHO cell culture performance	14:48	pH excursions impact CHO cell culture performance and antibody N-linked glycosylation.
30088083	6	83	theme	metabolic	817:825	arg1	rates					827:831	higher glucose and lactate metabolic rates	790:831	higher glucose and lactate metabolic rates than continuous addition	790:856	Lactate production increase was mainly caused by increased culture pH due to base addition, and bolus addition led to higher glucose and lactate metabolic rates than continuous addition.
30088083	6	84	theme	increased	721:729	arg1	pH					739:740	increased culture pH	721:740	increased culture pH due to base addition	721:761	Lactate production increase was mainly caused by increased culture pH due to base addition, and bolus addition led to higher glucose and lactate metabolic rates than continuous addition.
30088083	9	85	contain	had	1320:1322	arg2	response					1332:1339	minimal response	1324:1339	minimal response to cultivating pH. This suggests that the impact of pH excursions on antibody N-linked glycosylation is cell line specific and is closely related to cell line response to cultivating pH	1324:1525	On the other hand, there was no such substantial impact of pH excursions on galactosylation for the other two cell lines, both of which also had minimal response to cultivating pH. This suggests that the impact of pH excursions on antibody N-linked glycosylation is cell line specific and is closely related to cell line response to cultivating pH.
30088083	9	85	contain	had	1320:1322	arg1	both					1301:1304	both	1301:1304	both	1301:1304	On the other hand, there was no such substantial impact of pH excursions on galactosylation for the other two cell lines, both of which also had minimal response to cultivating pH. This suggests that the impact of pH excursions on antibody N-linked glycosylation is cell line specific and is closely related to cell line response to cultivating pH.
30088083	9	85	contain	had	1320:1322	arg1	lines					1294:1298	the other two cell lines	1275:1298	the other two cell lines	1275:1298	On the other hand, there was no such substantial impact of pH excursions on galactosylation for the other two cell lines, both of which also had minimal response to cultivating pH. This suggests that the impact of pH excursions on antibody N-linked glycosylation is cell line specific and is closely related to cell line response to cultivating pH.
31510952	10	0	theme	CNS	1674:1676	arg1	CONCLUSION					1688:1697	CONCLUSION	1688:1697	CONCLUSION	1688:1697	DISCUSSION This is the first study for the expression profile of N-oligosaccharides on MTX-resistant primary CNS lymphoma-derived cells HKBML and TK, and tumor tissues resected from patients with CNS lymphoma, CONCLUSION: These results propose a possibility that the differential expression of high-mannose types and sialylated A2G2F, A2G2FB, and A4G4F on the surface of CNS lymphomas may provide a hint for targets for diagnoses and treatments of the oligosaccharide type-specific lymphomas.
31510952	10	0	theme	CNS	1674:1676	arg1	lymphoma					1678:1685	CNS lymphoma	1674:1685	CNS lymphoma	1674:1685	DISCUSSION This is the first study for the expression profile of N-oligosaccharides on MTX-resistant primary CNS lymphoma-derived cells HKBML and TK, and tumor tissues resected from patients with CNS lymphoma, CONCLUSION: These results propose a possibility that the differential expression of high-mannose types and sialylated A2G2F, A2G2FB, and A4G4F on the surface of CNS lymphomas may provide a hint for targets for diagnoses and treatments of the oligosaccharide type-specific lymphomas.
31510952	10	1	theme	expression	1521:1530	arg1	profile					1532:1538	the expression profile	1517:1538	the expression profile of N-oligosaccharides	1517:1560	DISCUSSION This is the first study for the expression profile of N-oligosaccharides on MTX-resistant primary CNS lymphoma-derived cells HKBML and TK, and tumor tissues resected from patients with CNS lymphoma, CONCLUSION: These results propose a possibility that the differential expression of high-mannose types and sialylated A2G2F, A2G2FB, and A4G4F on the surface of CNS lymphomas may provide a hint for targets for diagnoses and treatments of the oligosaccharide type-specific lymphomas.
31510952	1	2	theme	immunoglobulin-like	309:327	arg1	lectins					329:335	immunoglobulin-like lectins	309:335	immunoglobulin-like lectins	309:335	BACKGROUND Oligosaccharides of glycoprotein, particularly negatively-charged sialylated N-glycans, on the surface of lymphomas play important roles in cell-cell interactions and bind immunoglobulin-like lectins, causing inflammatory responses and bioregulation.
31510952	10	3	from	A4G4F	1825:1829	arg1	surface					1838:1844	the surface	1834:1844	the surface of CNS lymphomas	1834:1861	DISCUSSION This is the first study for the expression profile of N-oligosaccharides on MTX-resistant primary CNS lymphoma-derived cells HKBML and TK, and tumor tissues resected from patients with CNS lymphoma, CONCLUSION: These results propose a possibility that the differential expression of high-mannose types and sialylated A2G2F, A2G2FB, and A4G4F on the surface of CNS lymphomas may provide a hint for targets for diagnoses and treatments of the oligosaccharide type-specific lymphomas.
31510952	4	4	link	N-linked	794:801	arg1	OYB					904:906	OYB	904:906	OYB	904:906	RESULTS We first generated methotrexate (MTX)-resistant cells derived from HKBML and TK as CNS lymphoma, and RAJI as non-CNS lymphoma and determined N-linked oligosaccharide structures in these cells and other non-CNS lymphoma-derived cells including A4/FUK, OYB, and HBL1.
31510952	4	4	link	N-linked	794:801	arg1	HBL1					913:916	HBL1	913:916	HBL1	913:916	RESULTS We first generated methotrexate (MTX)-resistant cells derived from HKBML and TK as CNS lymphoma, and RAJI as non-CNS lymphoma and determined N-linked oligosaccharide structures in these cells and other non-CNS lymphoma-derived cells including A4/FUK, OYB, and HBL1.
31510952	4	4	link	N-linked	794:801	arg1	A4/FUK					896:901	A4/FUK	896:901	A4/FUK	896:901	RESULTS We first generated methotrexate (MTX)-resistant cells derived from HKBML and TK as CNS lymphoma, and RAJI as non-CNS lymphoma and determined N-linked oligosaccharide structures in these cells and other non-CNS lymphoma-derived cells including A4/FUK, OYB, and HBL1.
31510952	4	4	link	N-linked	794:801	arg1	structures					819:828	N-linked oligosaccharide structures	794:828	N-linked oligosaccharide structures in these cells	794:843	RESULTS We first generated methotrexate (MTX)-resistant cells derived from HKBML and TK as CNS lymphoma, and RAJI as non-CNS lymphoma and determined N-linked oligosaccharide structures in these cells and other non-CNS lymphoma-derived cells including A4/FUK, OYB, and HBL1.
31510952	4	5	theme	CNS	736:738	arg1	lymphoma					740:747	CNS lymphoma	736:747	CNS lymphoma	736:747	RESULTS We first generated methotrexate (MTX)-resistant cells derived from HKBML and TK as CNS lymphoma, and RAJI as non-CNS lymphoma and determined N-linked oligosaccharide structures in these cells and other non-CNS lymphoma-derived cells including A4/FUK, OYB, and HBL1.
31510952	10	6	dep	cells	1608:1612	arg1	HKBML					1614:1618	HKBML	1614:1618	HKBML	1614:1618	DISCUSSION This is the first study for the expression profile of N-oligosaccharides on MTX-resistant primary CNS lymphoma-derived cells HKBML and TK, and tumor tissues resected from patients with CNS lymphoma, CONCLUSION: These results propose a possibility that the differential expression of high-mannose types and sialylated A2G2F, A2G2FB, and A4G4F on the surface of CNS lymphomas may provide a hint for targets for diagnoses and treatments of the oligosaccharide type-specific lymphomas.
31510952	10	6	dep	cells	1608:1612	arg1	cells					1608:1612	MTX-resistant primary CNS lymphoma-derived cells HKBML and TK	1565:1625	MTX-resistant primary CNS lymphoma-derived cells HKBML and TK	1565:1625	DISCUSSION This is the first study for the expression profile of N-oligosaccharides on MTX-resistant primary CNS lymphoma-derived cells HKBML and TK, and tumor tissues resected from patients with CNS lymphoma, CONCLUSION: These results propose a possibility that the differential expression of high-mannose types and sialylated A2G2F, A2G2FB, and A4G4F on the surface of CNS lymphomas may provide a hint for targets for diagnoses and treatments of the oligosaccharide type-specific lymphomas.
31510952	10	6	dep	cells	1608:1612	arg1	TK					1624:1625	TK	1624:1625	TK	1624:1625	DISCUSSION This is the first study for the expression profile of N-oligosaccharides on MTX-resistant primary CNS lymphoma-derived cells HKBML and TK, and tumor tissues resected from patients with CNS lymphoma, CONCLUSION: These results propose a possibility that the differential expression of high-mannose types and sialylated A2G2F, A2G2FB, and A4G4F on the surface of CNS lymphomas may provide a hint for targets for diagnoses and treatments of the oligosaccharide type-specific lymphomas.
31510952	5	7	theme	oligosaccharides	949:964	arg1	components					925:934	Major components	919:934	Major components of the total oligosaccharides	919:964	Major components of the total oligosaccharides were high-mannose type N-glycans, whose level increased in MTX-resistant HKBML and TK but decreased in MTX-resistant RAJI.
31510952	4	8	theme	oligosaccharide	803:817	arg1	OYB					904:906	OYB	904:906	OYB	904:906	RESULTS We first generated methotrexate (MTX)-resistant cells derived from HKBML and TK as CNS lymphoma, and RAJI as non-CNS lymphoma and determined N-linked oligosaccharide structures in these cells and other non-CNS lymphoma-derived cells including A4/FUK, OYB, and HBL1.
31510952	4	8	theme	oligosaccharide	803:817	arg1	HBL1					913:916	HBL1	913:916	HBL1	913:916	RESULTS We first generated methotrexate (MTX)-resistant cells derived from HKBML and TK as CNS lymphoma, and RAJI as non-CNS lymphoma and determined N-linked oligosaccharide structures in these cells and other non-CNS lymphoma-derived cells including A4/FUK, OYB, and HBL1.
31510952	4	8	theme	oligosaccharide	803:817	arg1	A4/FUK					896:901	A4/FUK	896:901	A4/FUK	896:901	RESULTS We first generated methotrexate (MTX)-resistant cells derived from HKBML and TK as CNS lymphoma, and RAJI as non-CNS lymphoma and determined N-linked oligosaccharide structures in these cells and other non-CNS lymphoma-derived cells including A4/FUK, OYB, and HBL1.
31510952	4	8	theme	oligosaccharide	803:817	arg1	structures					819:828	N-linked oligosaccharide structures	794:828	N-linked oligosaccharide structures in these cells	794:843	RESULTS We first generated methotrexate (MTX)-resistant cells derived from HKBML and TK as CNS lymphoma, and RAJI as non-CNS lymphoma and determined N-linked oligosaccharide structures in these cells and other non-CNS lymphoma-derived cells including A4/FUK, OYB, and HBL1.
31510952	10	9	dep	DISCUSSION	1478:1487	arg1	study					1507:1511	the first study	1497:1511	the first study for the expression profile of N-oligosaccharides on MTX-resistant primary CNS lymphoma-derived cells HKBML and TK, and tumor tissues resected from patients with CNS lymphoma, CONCLUSION	1497:1697	DISCUSSION This is the first study for the expression profile of N-oligosaccharides on MTX-resistant primary CNS lymphoma-derived cells HKBML and TK, and tumor tissues resected from patients with CNS lymphoma, CONCLUSION: These results propose a possibility that the differential expression of high-mannose types and sialylated A2G2F, A2G2FB, and A4G4F on the surface of CNS lymphomas may provide a hint for targets for diagnoses and treatments of the oligosaccharide type-specific lymphomas.
31510952	10	9	dep	DISCUSSION	1478:1487	arg1	provide					1867:1873	provide	1867:1873	may provide a hint for targets for diagnoses and treatments of the oligosaccharide type-specific lymphomas	1863:1968	DISCUSSION This is the first study for the expression profile of N-oligosaccharides on MTX-resistant primary CNS lymphoma-derived cells HKBML and TK, and tumor tissues resected from patients with CNS lymphoma, CONCLUSION: These results propose a possibility that the differential expression of high-mannose types and sialylated A2G2F, A2G2FB, and A4G4F on the surface of CNS lymphomas may provide a hint for targets for diagnoses and treatments of the oligosaccharide type-specific lymphomas.
31510952	10	9	dep	DISCUSSION	1478:1487	arg1	This					1489:1492	This	1489:1492	This	1489:1492	DISCUSSION This is the first study for the expression profile of N-oligosaccharides on MTX-resistant primary CNS lymphoma-derived cells HKBML and TK, and tumor tissues resected from patients with CNS lymphoma, CONCLUSION: These results propose a possibility that the differential expression of high-mannose types and sialylated A2G2F, A2G2FB, and A4G4F on the surface of CNS lymphomas may provide a hint for targets for diagnoses and treatments of the oligosaccharide type-specific lymphomas.
31510952	10	9	dep	DISCUSSION	1478:1487	arg1	propose					1714:1720	propose	1714:1720	propose a possibility that the differential expression of high-mannose types and sialylated A2G2F, A2G2FB	1714:1818	DISCUSSION This is the first study for the expression profile of N-oligosaccharides on MTX-resistant primary CNS lymphoma-derived cells HKBML and TK, and tumor tissues resected from patients with CNS lymphoma, CONCLUSION: These results propose a possibility that the differential expression of high-mannose types and sialylated A2G2F, A2G2FB, and A4G4F on the surface of CNS lymphomas may provide a hint for targets for diagnoses and treatments of the oligosaccharide type-specific lymphomas.
31510952	10	10	theme	types	1785:1789	arg1	expression					1758:1767	the differential expression	1741:1767	the differential expression of high-mannose types and sialylated A2G2F, A2G2FB	1741:1818	DISCUSSION This is the first study for the expression profile of N-oligosaccharides on MTX-resistant primary CNS lymphoma-derived cells HKBML and TK, and tumor tissues resected from patients with CNS lymphoma, CONCLUSION: These results propose a possibility that the differential expression of high-mannose types and sialylated A2G2F, A2G2FB, and A4G4F on the surface of CNS lymphomas may provide a hint for targets for diagnoses and treatments of the oligosaccharide type-specific lymphomas.
31510952	8	11	theme	non-resistant	1379:1391	arg1	cells					1393:1397	non-resistant cells	1379:1397	non-resistant cells	1379:1397	However, the ratios of these sialylated N-glycans slightly decreased against MTX-resistant compared to non-resistant cells.
31510952	8	12	theme	sialylated	1305:1314	arg1	N-glycans					1316:1324	these sialylated N-glycans	1299:1324	these sialylated N-glycans	1299:1324	However, the ratios of these sialylated N-glycans slightly decreased against MTX-resistant compared to non-resistant cells.
31510952	10	13	theme	differential	1745:1756	arg1	expression					1758:1767	the differential expression	1741:1767	the differential expression of high-mannose types and sialylated A2G2F, A2G2FB	1741:1818	DISCUSSION This is the first study for the expression profile of N-oligosaccharides on MTX-resistant primary CNS lymphoma-derived cells HKBML and TK, and tumor tissues resected from patients with CNS lymphoma, CONCLUSION: These results propose a possibility that the differential expression of high-mannose types and sialylated A2G2F, A2G2FB, and A4G4F on the surface of CNS lymphomas may provide a hint for targets for diagnoses and treatments of the oligosaccharide type-specific lymphomas.
31510952	4	14	attach	derived	707:713	arg1	TK					730:731	TK	730:731	TK	730:731	RESULTS We first generated methotrexate (MTX)-resistant cells derived from HKBML and TK as CNS lymphoma, and RAJI as non-CNS lymphoma and determined N-linked oligosaccharide structures in these cells and other non-CNS lymphoma-derived cells including A4/FUK, OYB, and HBL1.
31510952	4	14	attach	derived	707:713	arg2	cells					701:705	methotrexate (MTX)-resistant cells	672:705	methotrexate (MTX)-resistant cells derived from HKBML and TK as CNS lymphoma, and RAJI as non-CNS lymphoma	672:777	RESULTS We first generated methotrexate (MTX)-resistant cells derived from HKBML and TK as CNS lymphoma, and RAJI as non-CNS lymphoma and determined N-linked oligosaccharide structures in these cells and other non-CNS lymphoma-derived cells including A4/FUK, OYB, and HBL1.
31510952	4	14	attach	derived	707:713	arg1	RAJI					754:757	RAJI	754:757	RAJI	754:757	RESULTS We first generated methotrexate (MTX)-resistant cells derived from HKBML and TK as CNS lymphoma, and RAJI as non-CNS lymphoma and determined N-linked oligosaccharide structures in these cells and other non-CNS lymphoma-derived cells including A4/FUK, OYB, and HBL1.
31510952	4	14	attach	derived	707:713	arg1	HKBML					720:724	HKBML	720:724	HKBML	720:724	RESULTS We first generated methotrexate (MTX)-resistant cells derived from HKBML and TK as CNS lymphoma, and RAJI as non-CNS lymphoma and determined N-linked oligosaccharide structures in these cells and other non-CNS lymphoma-derived cells including A4/FUK, OYB, and HBL1.
31510952	1	15	theme	glycoprotein	157:168	arg1	Oligosaccharides					137:152	BACKGROUND Oligosaccharides	126:152	BACKGROUND Oligosaccharides of glycoprotein, particularly negatively-charged sialylated N-glycans, on the surface of lymphomas	126:251	BACKGROUND Oligosaccharides of glycoprotein, particularly negatively-charged sialylated N-glycans, on the surface of lymphomas play important roles in cell-cell interactions and bind immunoglobulin-like lectins, causing inflammatory responses and bioregulation.
31510952	4	16	theme	N-linked	794:801	arg1	OYB					904:906	OYB	904:906	OYB	904:906	RESULTS We first generated methotrexate (MTX)-resistant cells derived from HKBML and TK as CNS lymphoma, and RAJI as non-CNS lymphoma and determined N-linked oligosaccharide structures in these cells and other non-CNS lymphoma-derived cells including A4/FUK, OYB, and HBL1.
31510952	4	16	theme	N-linked	794:801	arg1	HBL1					913:916	HBL1	913:916	HBL1	913:916	RESULTS We first generated methotrexate (MTX)-resistant cells derived from HKBML and TK as CNS lymphoma, and RAJI as non-CNS lymphoma and determined N-linked oligosaccharide structures in these cells and other non-CNS lymphoma-derived cells including A4/FUK, OYB, and HBL1.
31510952	4	16	theme	N-linked	794:801	arg1	A4/FUK					896:901	A4/FUK	896:901	A4/FUK	896:901	RESULTS We first generated methotrexate (MTX)-resistant cells derived from HKBML and TK as CNS lymphoma, and RAJI as non-CNS lymphoma and determined N-linked oligosaccharide structures in these cells and other non-CNS lymphoma-derived cells including A4/FUK, OYB, and HBL1.
31510952	4	16	theme	N-linked	794:801	arg1	structures					819:828	N-linked oligosaccharide structures	794:828	N-linked oligosaccharide structures in these cells	794:843	RESULTS We first generated methotrexate (MTX)-resistant cells derived from HKBML and TK as CNS lymphoma, and RAJI as non-CNS lymphoma and determined N-linked oligosaccharide structures in these cells and other non-CNS lymphoma-derived cells including A4/FUK, OYB, and HBL1.
31510952	6	17	theme	sialylated	1106:1115	arg1	N-glycans					1144:1152	sialylated biantennary galactosylated N-glycans	1106:1152	sialylated biantennary galactosylated N-glycans with α1,6-fucosylation, A2G2F, and A2G2FB from HKBML, TK, and RAJI	1106:1219	We also detected sialylated biantennary galactosylated N-glycans with α1,6-fucosylation, A2G2F, and A2G2FB from HKBML, TK, and RAJI.
31510952	10	18	theme	tumor	1632:1636	arg1	tissues					1638:1644	tumor tissues	1632:1644	tumor tissues	1632:1644	DISCUSSION This is the first study for the expression profile of N-oligosaccharides on MTX-resistant primary CNS lymphoma-derived cells HKBML and TK, and tumor tissues resected from patients with CNS lymphoma, CONCLUSION: These results propose a possibility that the differential expression of high-mannose types and sialylated A2G2F, A2G2FB, and A4G4F on the surface of CNS lymphomas may provide a hint for targets for diagnoses and treatments of the oligosaccharide type-specific lymphomas.
31510952	0	19	theme	central	87:93	arg1	system					103:108	system	103:108	system	103:108	Differential expression of N-linked oligosaccharides in methotrexate-resistant primary central nervous system lymphoma cells.
31510952	10	20	theme	type-specific	1946:1958	arg1	lymphomas					1960:1968	the oligosaccharide type-specific lymphomas	1926:1968	the oligosaccharide type-specific lymphomas	1926:1968	DISCUSSION This is the first study for the expression profile of N-oligosaccharides on MTX-resistant primary CNS lymphoma-derived cells HKBML and TK, and tumor tissues resected from patients with CNS lymphoma, CONCLUSION: These results propose a possibility that the differential expression of high-mannose types and sialylated A2G2F, A2G2FB, and A4G4F on the surface of CNS lymphomas may provide a hint for targets for diagnoses and treatments of the oligosaccharide type-specific lymphomas.
31510952	4	21	from	cells	880:884	arg1	cells					839:843	these cells	833:843	these cells	833:843	RESULTS We first generated methotrexate (MTX)-resistant cells derived from HKBML and TK as CNS lymphoma, and RAJI as non-CNS lymphoma and determined N-linked oligosaccharide structures in these cells and other non-CNS lymphoma-derived cells including A4/FUK, OYB, and HBL1.
31510952	5	22	theme	high-mannose	971:982	arg1	N-glycans					989:997	high-mannose type N-glycans	971:997	high-mannose type N-glycans	971:997	Major components of the total oligosaccharides were high-mannose type N-glycans, whose level increased in MTX-resistant HKBML and TK but decreased in MTX-resistant RAJI.
31510952	10	23	theme	lymphoma-derived	1591:1606	arg1	HKBML					1614:1618	HKBML	1614:1618	HKBML	1614:1618	DISCUSSION This is the first study for the expression profile of N-oligosaccharides on MTX-resistant primary CNS lymphoma-derived cells HKBML and TK, and tumor tissues resected from patients with CNS lymphoma, CONCLUSION: These results propose a possibility that the differential expression of high-mannose types and sialylated A2G2F, A2G2FB, and A4G4F on the surface of CNS lymphomas may provide a hint for targets for diagnoses and treatments of the oligosaccharide type-specific lymphomas.
31510952	10	23	theme	lymphoma-derived	1591:1606	arg1	cells					1608:1612	MTX-resistant primary CNS lymphoma-derived cells HKBML and TK	1565:1625	MTX-resistant primary CNS lymphoma-derived cells HKBML and TK	1565:1625	DISCUSSION This is the first study for the expression profile of N-oligosaccharides on MTX-resistant primary CNS lymphoma-derived cells HKBML and TK, and tumor tissues resected from patients with CNS lymphoma, CONCLUSION: These results propose a possibility that the differential expression of high-mannose types and sialylated A2G2F, A2G2FB, and A4G4F on the surface of CNS lymphomas may provide a hint for targets for diagnoses and treatments of the oligosaccharide type-specific lymphomas.
31510952	10	23	theme	lymphoma-derived	1591:1606	arg1	TK					1624:1625	TK	1624:1625	TK	1624:1625	DISCUSSION This is the first study for the expression profile of N-oligosaccharides on MTX-resistant primary CNS lymphoma-derived cells HKBML and TK, and tumor tissues resected from patients with CNS lymphoma, CONCLUSION: These results propose a possibility that the differential expression of high-mannose types and sialylated A2G2F, A2G2FB, and A4G4F on the surface of CNS lymphomas may provide a hint for targets for diagnoses and treatments of the oligosaccharide type-specific lymphomas.
31510952	4	24	theme	non-CNS	762:768	arg1	lymphoma					770:777	non-CNS lymphoma	762:777	non-CNS lymphoma	762:777	RESULTS We first generated methotrexate (MTX)-resistant cells derived from HKBML and TK as CNS lymphoma, and RAJI as non-CNS lymphoma and determined N-linked oligosaccharide structures in these cells and other non-CNS lymphoma-derived cells including A4/FUK, OYB, and HBL1.
31510952	0	25	theme	system	103:108	arg1	cells					119:123	methotrexate-resistant primary central nervous system lymphoma cells	56:123	methotrexate-resistant primary central nervous system lymphoma cells	56:123	Differential expression of N-linked oligosaccharides in methotrexate-resistant primary central nervous system lymphoma cells.
31510952	10	26	theme	primary	1579:1585	arg1	HKBML					1614:1618	HKBML	1614:1618	HKBML	1614:1618	DISCUSSION This is the first study for the expression profile of N-oligosaccharides on MTX-resistant primary CNS lymphoma-derived cells HKBML and TK, and tumor tissues resected from patients with CNS lymphoma, CONCLUSION: These results propose a possibility that the differential expression of high-mannose types and sialylated A2G2F, A2G2FB, and A4G4F on the surface of CNS lymphomas may provide a hint for targets for diagnoses and treatments of the oligosaccharide type-specific lymphomas.
31510952	10	26	theme	primary	1579:1585	arg1	cells					1608:1612	MTX-resistant primary CNS lymphoma-derived cells HKBML and TK	1565:1625	MTX-resistant primary CNS lymphoma-derived cells HKBML and TK	1565:1625	DISCUSSION This is the first study for the expression profile of N-oligosaccharides on MTX-resistant primary CNS lymphoma-derived cells HKBML and TK, and tumor tissues resected from patients with CNS lymphoma, CONCLUSION: These results propose a possibility that the differential expression of high-mannose types and sialylated A2G2F, A2G2FB, and A4G4F on the surface of CNS lymphomas may provide a hint for targets for diagnoses and treatments of the oligosaccharide type-specific lymphomas.
31510952	10	26	theme	primary	1579:1585	arg1	TK					1624:1625	TK	1624:1625	TK	1624:1625	DISCUSSION This is the first study for the expression profile of N-oligosaccharides on MTX-resistant primary CNS lymphoma-derived cells HKBML and TK, and tumor tissues resected from patients with CNS lymphoma, CONCLUSION: These results propose a possibility that the differential expression of high-mannose types and sialylated A2G2F, A2G2FB, and A4G4F on the surface of CNS lymphomas may provide a hint for targets for diagnoses and treatments of the oligosaccharide type-specific lymphomas.
31510952	9	27	gly	α2,6-sialylated	1461:1475	arg1	oligosaccharides					1439:1454	almost all complex-type oligosaccharides	1415:1454	almost all complex-type oligosaccharides	1415:1454	Interestingly, almost all complex-type oligosaccharides were α2,6-sialylated.
31510952	10	28	from	patients	1660:1667	arg1	resected					1646:1653	resected	1646:1653	resected	1646:1653	DISCUSSION This is the first study for the expression profile of N-oligosaccharides on MTX-resistant primary CNS lymphoma-derived cells HKBML and TK, and tumor tissues resected from patients with CNS lymphoma, CONCLUSION: These results propose a possibility that the differential expression of high-mannose types and sialylated A2G2F, A2G2FB, and A4G4F on the surface of CNS lymphomas may provide a hint for targets for diagnoses and treatments of the oligosaccharide type-specific lymphomas.
31510952	4	29	theme	non-CNS	855:861	arg1	OYB					904:906	OYB	904:906	OYB	904:906	RESULTS We first generated methotrexate (MTX)-resistant cells derived from HKBML and TK as CNS lymphoma, and RAJI as non-CNS lymphoma and determined N-linked oligosaccharide structures in these cells and other non-CNS lymphoma-derived cells including A4/FUK, OYB, and HBL1.
31510952	4	29	theme	non-CNS	855:861	arg1	HBL1					913:916	HBL1	913:916	HBL1	913:916	RESULTS We first generated methotrexate (MTX)-resistant cells derived from HKBML and TK as CNS lymphoma, and RAJI as non-CNS lymphoma and determined N-linked oligosaccharide structures in these cells and other non-CNS lymphoma-derived cells including A4/FUK, OYB, and HBL1.
31510952	4	29	theme	non-CNS	855:861	arg1	A4/FUK					896:901	A4/FUK	896:901	A4/FUK	896:901	RESULTS We first generated methotrexate (MTX)-resistant cells derived from HKBML and TK as CNS lymphoma, and RAJI as non-CNS lymphoma and determined N-linked oligosaccharide structures in these cells and other non-CNS lymphoma-derived cells including A4/FUK, OYB, and HBL1.
31510952	4	29	theme	non-CNS	855:861	arg1	cells					880:884	other non-CNS lymphoma-derived cells	849:884	other non-CNS lymphoma-derived cells including A4/FUK, OYB, and HBL1	849:916	RESULTS We first generated methotrexate (MTX)-resistant cells derived from HKBML and TK as CNS lymphoma, and RAJI as non-CNS lymphoma and determined N-linked oligosaccharide structures in these cells and other non-CNS lymphoma-derived cells including A4/FUK, OYB, and HBL1.
31510952	4	30	dep	RESULTS	645:651	arg1	We					653:654	We	653:654	We	653:654	RESULTS We first generated methotrexate (MTX)-resistant cells derived from HKBML and TK as CNS lymphoma, and RAJI as non-CNS lymphoma and determined N-linked oligosaccharide structures in these cells and other non-CNS lymphoma-derived cells including A4/FUK, OYB, and HBL1.
31510952	2	31	theme	system	466:471	arg1	lymphoma					479:486	central nervous system (CNS) lymphoma	450:486	central nervous system (CNS) lymphoma	450:486	However, their characterizations have largely been unknown in central nervous system (CNS) lymphoma.
31510952	0	32	theme	Differential	0:11	arg1	expression					13:22	Differential expression	0:22	Differential expression of N-linked oligosaccharides in methotrexate-resistant primary central nervous system lymphoma cells.	0:124	Differential expression of N-linked oligosaccharides in methotrexate-resistant primary central nervous system lymphoma cells.
31510952	4	33	from	structures	819:828	arg1	cells					839:843	these cells	833:843	these cells	833:843	RESULTS We first generated methotrexate (MTX)-resistant cells derived from HKBML and TK as CNS lymphoma, and RAJI as non-CNS lymphoma and determined N-linked oligosaccharide structures in these cells and other non-CNS lymphoma-derived cells including A4/FUK, OYB, and HBL1.
31510952	5	34	theme	MTX-resistant	1025:1037	arg1	HKBML					1039:1043	MTX-resistant HKBML	1025:1043	MTX-resistant HKBML	1025:1043	Major components of the total oligosaccharides were high-mannose type N-glycans, whose level increased in MTX-resistant HKBML and TK but decreased in MTX-resistant RAJI.
31510952	10	35	theme	A2G2FB	1813:1818	arg1	expression					1758:1767	the differential expression	1741:1767	the differential expression of high-mannose types and sialylated A2G2F, A2G2FB	1741:1818	DISCUSSION This is the first study for the expression profile of N-oligosaccharides on MTX-resistant primary CNS lymphoma-derived cells HKBML and TK, and tumor tissues resected from patients with CNS lymphoma, CONCLUSION: These results propose a possibility that the differential expression of high-mannose types and sialylated A2G2F, A2G2FB, and A4G4F on the surface of CNS lymphomas may provide a hint for targets for diagnoses and treatments of the oligosaccharide type-specific lymphomas.
31510952	3	36	theme	expression	519:528	arg1	patterns					530:537	expression patterns	519:537	expression patterns of N-linked oligosaccharides of glycoproteins	519:583	METHODS Here, we investigated expression patterns of N-linked oligosaccharides of glycoproteins in cells derived from CNS lymphomas and clinical specimens.
31510952	0	37	theme	oligosaccharides	36:51	arg1	expression					13:22	Differential expression	0:22	Differential expression of N-linked oligosaccharides in methotrexate-resistant primary central nervous system lymphoma cells.	0:124	Differential expression of N-linked oligosaccharides in methotrexate-resistant primary central nervous system lymphoma cells.
31510952	6	38	theme	galactosylated	1129:1142	arg1	N-glycans					1144:1152	sialylated biantennary galactosylated N-glycans	1106:1152	sialylated biantennary galactosylated N-glycans with α1,6-fucosylation, A2G2F, and A2G2FB from HKBML, TK, and RAJI	1106:1219	We also detected sialylated biantennary galactosylated N-glycans with α1,6-fucosylation, A2G2F, and A2G2FB from HKBML, TK, and RAJI.
31510952	6	39	from	TK	1208:1209	arg1	N-glycans					1144:1152	sialylated biantennary galactosylated N-glycans	1106:1152	sialylated biantennary galactosylated N-glycans with α1,6-fucosylation, A2G2F, and A2G2FB from HKBML, TK, and RAJI	1106:1219	We also detected sialylated biantennary galactosylated N-glycans with α1,6-fucosylation, A2G2F, and A2G2FB from HKBML, TK, and RAJI.
31510952	3	40	theme	glycoproteins	571:583	arg1	oligosaccharides					551:566	N-linked oligosaccharides	542:566	N-linked oligosaccharides of glycoproteins	542:583	METHODS Here, we investigated expression patterns of N-linked oligosaccharides of glycoproteins in cells derived from CNS lymphomas and clinical specimens.
31510952	0	41	theme	methotrexate-resistant	56:77	arg1	cells					119:123	methotrexate-resistant primary central nervous system lymphoma cells	56:123	methotrexate-resistant primary central nervous system lymphoma cells	56:123	Differential expression of N-linked oligosaccharides in methotrexate-resistant primary central nervous system lymphoma cells.
31510952	6	42	with	N-glycans	1144:1152	arg1	A2G2F					1178:1182	A2G2F	1178:1182	A2G2F	1178:1182	We also detected sialylated biantennary galactosylated N-glycans with α1,6-fucosylation, A2G2F, and A2G2FB from HKBML, TK, and RAJI.
31510952	6	42	with	N-glycans	1144:1152	arg1	A2G2FB					1189:1194	A2G2FB	1189:1194	A2G2FB	1189:1194	We also detected sialylated biantennary galactosylated N-glycans with α1,6-fucosylation, A2G2F, and A2G2FB from HKBML, TK, and RAJI.
31510952	6	42	with	N-glycans	1144:1152	arg1	α1,6-fucosylation					1159:1175	α1,6-fucosylation	1159:1175	α1,6-fucosylation	1159:1175	We also detected sialylated biantennary galactosylated N-glycans with α1,6-fucosylation, A2G2F, and A2G2FB from HKBML, TK, and RAJI.
31510952	3	43	attach	derived	594:600	arg1	lymphomas					611:619	CNS lymphomas	607:619	CNS lymphomas	607:619	METHODS Here, we investigated expression patterns of N-linked oligosaccharides of glycoproteins in cells derived from CNS lymphomas and clinical specimens.
31510952	3	43	attach	derived	594:600	arg1	specimens					634:642	clinical specimens	625:642	clinical specimens	625:642	METHODS Here, we investigated expression patterns of N-linked oligosaccharides of glycoproteins in cells derived from CNS lymphomas and clinical specimens.
31510952	3	43	attach	derived	594:600	arg2	cells					588:592	cells	588:592	cells derived from CNS lymphomas and clinical specimens	588:642	METHODS Here, we investigated expression patterns of N-linked oligosaccharides of glycoproteins in cells derived from CNS lymphomas and clinical specimens.
31510952	10	44	from	study	1507:1511	arg1	HKBML					1614:1618	HKBML	1614:1618	HKBML	1614:1618	DISCUSSION This is the first study for the expression profile of N-oligosaccharides on MTX-resistant primary CNS lymphoma-derived cells HKBML and TK, and tumor tissues resected from patients with CNS lymphoma, CONCLUSION: These results propose a possibility that the differential expression of high-mannose types and sialylated A2G2F, A2G2FB, and A4G4F on the surface of CNS lymphomas may provide a hint for targets for diagnoses and treatments of the oligosaccharide type-specific lymphomas.
31510952	10	44	from	study	1507:1511	arg1	tissues					1638:1644	tumor tissues	1632:1644	tumor tissues	1632:1644	DISCUSSION This is the first study for the expression profile of N-oligosaccharides on MTX-resistant primary CNS lymphoma-derived cells HKBML and TK, and tumor tissues resected from patients with CNS lymphoma, CONCLUSION: These results propose a possibility that the differential expression of high-mannose types and sialylated A2G2F, A2G2FB, and A4G4F on the surface of CNS lymphomas may provide a hint for targets for diagnoses and treatments of the oligosaccharide type-specific lymphomas.
31510952	10	44	from	study	1507:1511	arg1	cells					1608:1612	MTX-resistant primary CNS lymphoma-derived cells HKBML and TK	1565:1625	MTX-resistant primary CNS lymphoma-derived cells HKBML and TK	1565:1625	DISCUSSION This is the first study for the expression profile of N-oligosaccharides on MTX-resistant primary CNS lymphoma-derived cells HKBML and TK, and tumor tissues resected from patients with CNS lymphoma, CONCLUSION: These results propose a possibility that the differential expression of high-mannose types and sialylated A2G2F, A2G2FB, and A4G4F on the surface of CNS lymphomas may provide a hint for targets for diagnoses and treatments of the oligosaccharide type-specific lymphomas.
31510952	10	44	from	study	1507:1511	arg1	TK					1624:1625	TK	1624:1625	TK	1624:1625	DISCUSSION This is the first study for the expression profile of N-oligosaccharides on MTX-resistant primary CNS lymphoma-derived cells HKBML and TK, and tumor tissues resected from patients with CNS lymphoma, CONCLUSION: These results propose a possibility that the differential expression of high-mannose types and sialylated A2G2F, A2G2FB, and A4G4F on the surface of CNS lymphomas may provide a hint for targets for diagnoses and treatments of the oligosaccharide type-specific lymphomas.
31510952	3	45	theme	oligosaccharides	551:566	arg1	patterns					530:537	expression patterns	519:537	expression patterns of N-linked oligosaccharides of glycoproteins	519:583	METHODS Here, we investigated expression patterns of N-linked oligosaccharides of glycoproteins in cells derived from CNS lymphomas and clinical specimens.
31510952	1	46	from	Oligosaccharides	137:152	arg1	surface					232:238	the surface	228:238	the surface of lymphomas	228:251	BACKGROUND Oligosaccharides of glycoprotein, particularly negatively-charged sialylated N-glycans, on the surface of lymphomas play important roles in cell-cell interactions and bind immunoglobulin-like lectins, causing inflammatory responses and bioregulation.
31510952	2	47	from	lymphoma	479:486	arg1	unknown					439:445	unknown	439:445	unknown	439:445	However, their characterizations have largely been unknown in central nervous system (CNS) lymphoma.
31510952	1	48	theme	sialylated	203:212	arg1	N-glycans					214:222	particularly negatively-charged sialylated N-glycans	171:222	particularly negatively-charged sialylated N-glycans	171:222	BACKGROUND Oligosaccharides of glycoprotein, particularly negatively-charged sialylated N-glycans, on the surface of lymphomas play important roles in cell-cell interactions and bind immunoglobulin-like lectins, causing inflammatory responses and bioregulation.
31510952	1	48	theme	sialylated	203:212	arg1	glycoprotein					157:168	glycoprotein	157:168	glycoprotein	157:168	BACKGROUND Oligosaccharides of glycoprotein, particularly negatively-charged sialylated N-glycans, on the surface of lymphomas play important roles in cell-cell interactions and bind immunoglobulin-like lectins, causing inflammatory responses and bioregulation.
31510952	4	49	link	lymphoma-derived	863:878	arg1	OYB					904:906	OYB	904:906	OYB	904:906	RESULTS We first generated methotrexate (MTX)-resistant cells derived from HKBML and TK as CNS lymphoma, and RAJI as non-CNS lymphoma and determined N-linked oligosaccharide structures in these cells and other non-CNS lymphoma-derived cells including A4/FUK, OYB, and HBL1.
31510952	4	49	link	lymphoma-derived	863:878	arg1	HBL1					913:916	HBL1	913:916	HBL1	913:916	RESULTS We first generated methotrexate (MTX)-resistant cells derived from HKBML and TK as CNS lymphoma, and RAJI as non-CNS lymphoma and determined N-linked oligosaccharide structures in these cells and other non-CNS lymphoma-derived cells including A4/FUK, OYB, and HBL1.
31510952	4	49	link	lymphoma-derived	863:878	arg1	A4/FUK					896:901	A4/FUK	896:901	A4/FUK	896:901	RESULTS We first generated methotrexate (MTX)-resistant cells derived from HKBML and TK as CNS lymphoma, and RAJI as non-CNS lymphoma and determined N-linked oligosaccharide structures in these cells and other non-CNS lymphoma-derived cells including A4/FUK, OYB, and HBL1.
31510952	4	49	link	lymphoma-derived	863:878	arg1	cells					880:884	other non-CNS lymphoma-derived cells	849:884	other non-CNS lymphoma-derived cells including A4/FUK, OYB, and HBL1	849:916	RESULTS We first generated methotrexate (MTX)-resistant cells derived from HKBML and TK as CNS lymphoma, and RAJI as non-CNS lymphoma and determined N-linked oligosaccharide structures in these cells and other non-CNS lymphoma-derived cells including A4/FUK, OYB, and HBL1.
31510952	2	50	theme	central	450:456	arg1	CNS					474:476	CNS	474:476	CNS	474:476	However, their characterizations have largely been unknown in central nervous system (CNS) lymphoma.
31510952	2	50	theme	central	450:456	arg1	system					466:471	central nervous system	450:471	central nervous system (CNS) lymphoma	450:486	However, their characterizations have largely been unknown in central nervous system (CNS) lymphoma.
31510952	6	51	from	HKBML	1201:1205	arg1	N-glycans					1144:1152	sialylated biantennary galactosylated N-glycans	1106:1152	sialylated biantennary galactosylated N-glycans with α1,6-fucosylation, A2G2F, and A2G2FB from HKBML, TK, and RAJI	1106:1219	We also detected sialylated biantennary galactosylated N-glycans with α1,6-fucosylation, A2G2F, and A2G2FB from HKBML, TK, and RAJI.
31510952	10	52	theme	CNS	1849:1851	arg1	lymphomas					1853:1861	CNS lymphomas	1849:1861	CNS lymphomas	1849:1861	DISCUSSION This is the first study for the expression profile of N-oligosaccharides on MTX-resistant primary CNS lymphoma-derived cells HKBML and TK, and tumor tissues resected from patients with CNS lymphoma, CONCLUSION: These results propose a possibility that the differential expression of high-mannose types and sialylated A2G2F, A2G2FB, and A4G4F on the surface of CNS lymphomas may provide a hint for targets for diagnoses and treatments of the oligosaccharide type-specific lymphomas.
31510952	0	53	link	N-linked	27:34	arg1	oligosaccharides					36:51	N-linked oligosaccharides	27:51	N-linked oligosaccharides	27:51	Differential expression of N-linked oligosaccharides in methotrexate-resistant primary central nervous system lymphoma cells.
31510952	3	54	theme	clinical	625:632	arg1	specimens					634:642	clinical specimens	625:642	clinical specimens	625:642	METHODS Here, we investigated expression patterns of N-linked oligosaccharides of glycoproteins in cells derived from CNS lymphomas and clinical specimens.
31510952	3	55	link	N-linked	542:549	arg1	oligosaccharides					551:566	N-linked oligosaccharides	542:566	N-linked oligosaccharides of glycoproteins	542:583	METHODS Here, we investigated expression patterns of N-linked oligosaccharides of glycoproteins in cells derived from CNS lymphomas and clinical specimens.
31510952	7	56	theme	Sialylated	1222:1231	arg1	A4G4F					1233:1237	Sialylated A4G4F	1222:1237	Sialylated A4G4F	1222:1237	Sialylated A4G4F was specifically isolated from RAJI.
31510952	2	57	from	unknown	439:445	arg1	lymphoma					479:486	central nervous system (CNS) lymphoma	450:486	central nervous system (CNS) lymphoma	450:486	However, their characterizations have largely been unknown in central nervous system (CNS) lymphoma.
31510952	8	58	theme	N-glycans	1316:1324	arg1	ratios					1289:1294	the ratios	1285:1294	the ratios of these sialylated N-glycans	1285:1324	However, the ratios of these sialylated N-glycans slightly decreased against MTX-resistant compared to non-resistant cells.
31510952	10	59	theme	N-oligosaccharides	1543:1560	arg1	profile					1532:1538	the expression profile	1517:1538	the expression profile of N-oligosaccharides	1517:1560	DISCUSSION This is the first study for the expression profile of N-oligosaccharides on MTX-resistant primary CNS lymphoma-derived cells HKBML and TK, and tumor tissues resected from patients with CNS lymphoma, CONCLUSION: These results propose a possibility that the differential expression of high-mannose types and sialylated A2G2F, A2G2FB, and A4G4F on the surface of CNS lymphomas may provide a hint for targets for diagnoses and treatments of the oligosaccharide type-specific lymphomas.
31510952	5	60	theme	total	943:947	arg1	oligosaccharides					949:964	the total oligosaccharides	939:964	the total oligosaccharides	939:964	Major components of the total oligosaccharides were high-mannose type N-glycans, whose level increased in MTX-resistant HKBML and TK but decreased in MTX-resistant RAJI.
31510952	1	61	gly	glycoprotein	157:168	arg1	N-glycans					214:222	particularly negatively-charged sialylated N-glycans	171:222	particularly negatively-charged sialylated N-glycans	171:222	BACKGROUND Oligosaccharides of glycoprotein, particularly negatively-charged sialylated N-glycans, on the surface of lymphomas play important roles in cell-cell interactions and bind immunoglobulin-like lectins, causing inflammatory responses and bioregulation.
31510952	1	61	gly	glycoprotein	157:168	arg1	glycoprotein					157:168	glycoprotein	157:168	glycoprotein	157:168	BACKGROUND Oligosaccharides of glycoprotein, particularly negatively-charged sialylated N-glycans, on the surface of lymphomas play important roles in cell-cell interactions and bind immunoglobulin-like lectins, causing inflammatory responses and bioregulation.
31510952	8	62	gly	sialylated	1305:1314	arg1	N-glycans					1316:1324	these sialylated N-glycans	1299:1324	these sialylated N-glycans	1299:1324	However, the ratios of these sialylated N-glycans slightly decreased against MTX-resistant compared to non-resistant cells.
31510952	1	63	theme	lymphomas	243:251	arg1	surface					232:238	the surface	228:238	the surface of lymphomas	228:251	BACKGROUND Oligosaccharides of glycoprotein, particularly negatively-charged sialylated N-glycans, on the surface of lymphomas play important roles in cell-cell interactions and bind immunoglobulin-like lectins, causing inflammatory responses and bioregulation.
31510952	10	64	theme	high-mannose	1772:1783	arg1	types					1785:1789	high-mannose types	1772:1789	high-mannose types	1772:1789	DISCUSSION This is the first study for the expression profile of N-oligosaccharides on MTX-resistant primary CNS lymphoma-derived cells HKBML and TK, and tumor tissues resected from patients with CNS lymphoma, CONCLUSION: These results propose a possibility that the differential expression of high-mannose types and sialylated A2G2F, A2G2FB, and A4G4F on the surface of CNS lymphomas may provide a hint for targets for diagnoses and treatments of the oligosaccharide type-specific lymphomas.
31510952	5	65	theme	Major	919:923	arg1	components					925:934	Major components	919:934	Major components of the total oligosaccharides	919:964	Major components of the total oligosaccharides were high-mannose type N-glycans, whose level increased in MTX-resistant HKBML and TK but decreased in MTX-resistant RAJI.
31510952	1	66	gly	sialylated	203:212	arg1	N-glycans					214:222	particularly negatively-charged sialylated N-glycans	171:222	particularly negatively-charged sialylated N-glycans	171:222	BACKGROUND Oligosaccharides of glycoprotein, particularly negatively-charged sialylated N-glycans, on the surface of lymphomas play important roles in cell-cell interactions and bind immunoglobulin-like lectins, causing inflammatory responses and bioregulation.
31510952	1	66	gly	sialylated	203:212	arg1	glycoprotein					157:168	glycoprotein	157:168	glycoprotein	157:168	BACKGROUND Oligosaccharides of glycoprotein, particularly negatively-charged sialylated N-glycans, on the surface of lymphomas play important roles in cell-cell interactions and bind immunoglobulin-like lectins, causing inflammatory responses and bioregulation.
31510952	6	67	theme	biantennary	1117:1127	arg1	N-glycans					1144:1152	sialylated biantennary galactosylated N-glycans	1106:1152	sialylated biantennary galactosylated N-glycans with α1,6-fucosylation, A2G2F, and A2G2FB from HKBML, TK, and RAJI	1106:1219	We also detected sialylated biantennary galactosylated N-glycans with α1,6-fucosylation, A2G2F, and A2G2FB from HKBML, TK, and RAJI.
31510952	10	68	link	lymphoma-derived	1591:1606	arg1	HKBML					1614:1618	HKBML	1614:1618	HKBML	1614:1618	DISCUSSION This is the first study for the expression profile of N-oligosaccharides on MTX-resistant primary CNS lymphoma-derived cells HKBML and TK, and tumor tissues resected from patients with CNS lymphoma, CONCLUSION: These results propose a possibility that the differential expression of high-mannose types and sialylated A2G2F, A2G2FB, and A4G4F on the surface of CNS lymphomas may provide a hint for targets for diagnoses and treatments of the oligosaccharide type-specific lymphomas.
31510952	10	68	link	lymphoma-derived	1591:1606	arg1	cells					1608:1612	MTX-resistant primary CNS lymphoma-derived cells HKBML and TK	1565:1625	MTX-resistant primary CNS lymphoma-derived cells HKBML and TK	1565:1625	DISCUSSION This is the first study for the expression profile of N-oligosaccharides on MTX-resistant primary CNS lymphoma-derived cells HKBML and TK, and tumor tissues resected from patients with CNS lymphoma, CONCLUSION: These results propose a possibility that the differential expression of high-mannose types and sialylated A2G2F, A2G2FB, and A4G4F on the surface of CNS lymphomas may provide a hint for targets for diagnoses and treatments of the oligosaccharide type-specific lymphomas.
31510952	10	68	link	lymphoma-derived	1591:1606	arg1	TK					1624:1625	TK	1624:1625	TK	1624:1625	DISCUSSION This is the first study for the expression profile of N-oligosaccharides on MTX-resistant primary CNS lymphoma-derived cells HKBML and TK, and tumor tissues resected from patients with CNS lymphoma, CONCLUSION: These results propose a possibility that the differential expression of high-mannose types and sialylated A2G2F, A2G2FB, and A4G4F on the surface of CNS lymphomas may provide a hint for targets for diagnoses and treatments of the oligosaccharide type-specific lymphomas.
31510952	0	69	theme	nervous	95:101	arg1	system					103:108	system	103:108	system	103:108	Differential expression of N-linked oligosaccharides in methotrexate-resistant primary central nervous system lymphoma cells.
31510952	10	70	theme	lymphomas	1960:1968	arg1	treatments					1912:1921	treatments	1912:1921	treatments	1912:1921	DISCUSSION This is the first study for the expression profile of N-oligosaccharides on MTX-resistant primary CNS lymphoma-derived cells HKBML and TK, and tumor tissues resected from patients with CNS lymphoma, CONCLUSION: These results propose a possibility that the differential expression of high-mannose types and sialylated A2G2F, A2G2FB, and A4G4F on the surface of CNS lymphomas may provide a hint for targets for diagnoses and treatments of the oligosaccharide type-specific lymphomas.
31510952	10	70	theme	lymphomas	1960:1968	arg1	diagnoses					1898:1906	diagnoses	1898:1906	diagnoses	1898:1906	DISCUSSION This is the first study for the expression profile of N-oligosaccharides on MTX-resistant primary CNS lymphoma-derived cells HKBML and TK, and tumor tissues resected from patients with CNS lymphoma, CONCLUSION: These results propose a possibility that the differential expression of high-mannose types and sialylated A2G2F, A2G2FB, and A4G4F on the surface of CNS lymphomas may provide a hint for targets for diagnoses and treatments of the oligosaccharide type-specific lymphomas.
31510952	1	71	theme	negatively-charged	184:201	arg1	N-glycans					214:222	particularly negatively-charged sialylated N-glycans	171:222	particularly negatively-charged sialylated N-glycans	171:222	BACKGROUND Oligosaccharides of glycoprotein, particularly negatively-charged sialylated N-glycans, on the surface of lymphomas play important roles in cell-cell interactions and bind immunoglobulin-like lectins, causing inflammatory responses and bioregulation.
31510952	1	71	theme	negatively-charged	184:201	arg1	glycoprotein					157:168	glycoprotein	157:168	glycoprotein	157:168	BACKGROUND Oligosaccharides of glycoprotein, particularly negatively-charged sialylated N-glycans, on the surface of lymphomas play important roles in cell-cell interactions and bind immunoglobulin-like lectins, causing inflammatory responses and bioregulation.
31510952	0	72	theme	lymphoma	110:117	arg1	cells					119:123	methotrexate-resistant primary central nervous system lymphoma cells	56:123	methotrexate-resistant primary central nervous system lymphoma cells	56:123	Differential expression of N-linked oligosaccharides in methotrexate-resistant primary central nervous system lymphoma cells.
31510952	5	73	theme	type	984:987	arg1	N-glycans					989:997	high-mannose type N-glycans	971:997	high-mannose type N-glycans	971:997	Major components of the total oligosaccharides were high-mannose type N-glycans, whose level increased in MTX-resistant HKBML and TK but decreased in MTX-resistant RAJI.
31510952	0	74	from	expression	13:22	arg1	cells					119:123	methotrexate-resistant primary central nervous system lymphoma cells	56:123	methotrexate-resistant primary central nervous system lymphoma cells	56:123	Differential expression of N-linked oligosaccharides in methotrexate-resistant primary central nervous system lymphoma cells.
31510952	10	75	theme	CNS	1587:1589	arg1	HKBML					1614:1618	HKBML	1614:1618	HKBML	1614:1618	DISCUSSION This is the first study for the expression profile of N-oligosaccharides on MTX-resistant primary CNS lymphoma-derived cells HKBML and TK, and tumor tissues resected from patients with CNS lymphoma, CONCLUSION: These results propose a possibility that the differential expression of high-mannose types and sialylated A2G2F, A2G2FB, and A4G4F on the surface of CNS lymphomas may provide a hint for targets for diagnoses and treatments of the oligosaccharide type-specific lymphomas.
31510952	10	75	theme	CNS	1587:1589	arg1	cells					1608:1612	MTX-resistant primary CNS lymphoma-derived cells HKBML and TK	1565:1625	MTX-resistant primary CNS lymphoma-derived cells HKBML and TK	1565:1625	DISCUSSION This is the first study for the expression profile of N-oligosaccharides on MTX-resistant primary CNS lymphoma-derived cells HKBML and TK, and tumor tissues resected from patients with CNS lymphoma, CONCLUSION: These results propose a possibility that the differential expression of high-mannose types and sialylated A2G2F, A2G2FB, and A4G4F on the surface of CNS lymphomas may provide a hint for targets for diagnoses and treatments of the oligosaccharide type-specific lymphomas.
31510952	10	75	theme	CNS	1587:1589	arg1	TK					1624:1625	TK	1624:1625	TK	1624:1625	DISCUSSION This is the first study for the expression profile of N-oligosaccharides on MTX-resistant primary CNS lymphoma-derived cells HKBML and TK, and tumor tissues resected from patients with CNS lymphoma, CONCLUSION: These results propose a possibility that the differential expression of high-mannose types and sialylated A2G2F, A2G2FB, and A4G4F on the surface of CNS lymphomas may provide a hint for targets for diagnoses and treatments of the oligosaccharide type-specific lymphomas.
31510952	10	76	with	patients	1660:1667	arg1	CONCLUSION					1688:1697	CONCLUSION	1688:1697	CONCLUSION	1688:1697	DISCUSSION This is the first study for the expression profile of N-oligosaccharides on MTX-resistant primary CNS lymphoma-derived cells HKBML and TK, and tumor tissues resected from patients with CNS lymphoma, CONCLUSION: These results propose a possibility that the differential expression of high-mannose types and sialylated A2G2F, A2G2FB, and A4G4F on the surface of CNS lymphomas may provide a hint for targets for diagnoses and treatments of the oligosaccharide type-specific lymphomas.
31510952	10	76	with	patients	1660:1667	arg1	lymphoma					1678:1685	CNS lymphoma	1674:1685	CNS lymphoma	1674:1685	DISCUSSION This is the first study for the expression profile of N-oligosaccharides on MTX-resistant primary CNS lymphoma-derived cells HKBML and TK, and tumor tissues resected from patients with CNS lymphoma, CONCLUSION: These results propose a possibility that the differential expression of high-mannose types and sialylated A2G2F, A2G2FB, and A4G4F on the surface of CNS lymphomas may provide a hint for targets for diagnoses and treatments of the oligosaccharide type-specific lymphomas.
31510952	10	77	theme	first	1501:1505	arg1	study					1507:1511	the first study	1497:1511	the first study for the expression profile of N-oligosaccharides on MTX-resistant primary CNS lymphoma-derived cells HKBML and TK, and tumor tissues resected from patients with CNS lymphoma, CONCLUSION	1497:1697	DISCUSSION This is the first study for the expression profile of N-oligosaccharides on MTX-resistant primary CNS lymphoma-derived cells HKBML and TK, and tumor tissues resected from patients with CNS lymphoma, CONCLUSION: These results propose a possibility that the differential expression of high-mannose types and sialylated A2G2F, A2G2FB, and A4G4F on the surface of CNS lymphomas may provide a hint for targets for diagnoses and treatments of the oligosaccharide type-specific lymphomas.
31510952	10	77	theme	first	1501:1505	arg1	This					1489:1492	This	1489:1492	This	1489:1492	DISCUSSION This is the first study for the expression profile of N-oligosaccharides on MTX-resistant primary CNS lymphoma-derived cells HKBML and TK, and tumor tissues resected from patients with CNS lymphoma, CONCLUSION: These results propose a possibility that the differential expression of high-mannose types and sialylated A2G2F, A2G2FB, and A4G4F on the surface of CNS lymphomas may provide a hint for targets for diagnoses and treatments of the oligosaccharide type-specific lymphomas.
31510952	10	78	theme	MTX-resistant	1565:1577	arg1	HKBML					1614:1618	HKBML	1614:1618	HKBML	1614:1618	DISCUSSION This is the first study for the expression profile of N-oligosaccharides on MTX-resistant primary CNS lymphoma-derived cells HKBML and TK, and tumor tissues resected from patients with CNS lymphoma, CONCLUSION: These results propose a possibility that the differential expression of high-mannose types and sialylated A2G2F, A2G2FB, and A4G4F on the surface of CNS lymphomas may provide a hint for targets for diagnoses and treatments of the oligosaccharide type-specific lymphomas.
31510952	10	78	theme	MTX-resistant	1565:1577	arg1	cells					1608:1612	MTX-resistant primary CNS lymphoma-derived cells HKBML and TK	1565:1625	MTX-resistant primary CNS lymphoma-derived cells HKBML and TK	1565:1625	DISCUSSION This is the first study for the expression profile of N-oligosaccharides on MTX-resistant primary CNS lymphoma-derived cells HKBML and TK, and tumor tissues resected from patients with CNS lymphoma, CONCLUSION: These results propose a possibility that the differential expression of high-mannose types and sialylated A2G2F, A2G2FB, and A4G4F on the surface of CNS lymphomas may provide a hint for targets for diagnoses and treatments of the oligosaccharide type-specific lymphomas.
31510952	10	78	theme	MTX-resistant	1565:1577	arg1	TK					1624:1625	TK	1624:1625	TK	1624:1625	DISCUSSION This is the first study for the expression profile of N-oligosaccharides on MTX-resistant primary CNS lymphoma-derived cells HKBML and TK, and tumor tissues resected from patients with CNS lymphoma, CONCLUSION: These results propose a possibility that the differential expression of high-mannose types and sialylated A2G2F, A2G2FB, and A4G4F on the surface of CNS lymphomas may provide a hint for targets for diagnoses and treatments of the oligosaccharide type-specific lymphomas.
31510952	9	79	theme	all	1422:1424	arg1	oligosaccharides					1439:1454	almost all complex-type oligosaccharides	1415:1454	almost all complex-type oligosaccharides	1415:1454	Interestingly, almost all complex-type oligosaccharides were α2,6-sialylated.
31510952	4	80	theme	other	849:853	arg1	OYB					904:906	OYB	904:906	OYB	904:906	RESULTS We first generated methotrexate (MTX)-resistant cells derived from HKBML and TK as CNS lymphoma, and RAJI as non-CNS lymphoma and determined N-linked oligosaccharide structures in these cells and other non-CNS lymphoma-derived cells including A4/FUK, OYB, and HBL1.
31510952	4	80	theme	other	849:853	arg1	HBL1					913:916	HBL1	913:916	HBL1	913:916	RESULTS We first generated methotrexate (MTX)-resistant cells derived from HKBML and TK as CNS lymphoma, and RAJI as non-CNS lymphoma and determined N-linked oligosaccharide structures in these cells and other non-CNS lymphoma-derived cells including A4/FUK, OYB, and HBL1.
31510952	4	80	theme	other	849:853	arg1	A4/FUK					896:901	A4/FUK	896:901	A4/FUK	896:901	RESULTS We first generated methotrexate (MTX)-resistant cells derived from HKBML and TK as CNS lymphoma, and RAJI as non-CNS lymphoma and determined N-linked oligosaccharide structures in these cells and other non-CNS lymphoma-derived cells including A4/FUK, OYB, and HBL1.
31510952	4	80	theme	other	849:853	arg1	cells					880:884	other non-CNS lymphoma-derived cells	849:884	other non-CNS lymphoma-derived cells including A4/FUK, OYB, and HBL1	849:916	RESULTS We first generated methotrexate (MTX)-resistant cells derived from HKBML and TK as CNS lymphoma, and RAJI as non-CNS lymphoma and determined N-linked oligosaccharide structures in these cells and other non-CNS lymphoma-derived cells including A4/FUK, OYB, and HBL1.
31510952	3	81	dep	METHODS	489:495	arg1	investigated					506:517	investigated	506:517	investigated expression patterns of N-linked oligosaccharides of glycoproteins in cells derived from CNS lymphomas and clinical specimens	506:642	METHODS Here, we investigated expression patterns of N-linked oligosaccharides of glycoproteins in cells derived from CNS lymphomas and clinical specimens.
31510952	7	82	attach	isolated	1256:1263	arg1	RAJI					1270:1273	RAJI	1270:1273	RAJI	1270:1273	Sialylated A4G4F was specifically isolated from RAJI.
31510952	7	82	attach	isolated	1256:1263	arg2	A4G4F					1233:1237	Sialylated A4G4F	1222:1237	Sialylated A4G4F	1222:1237	Sialylated A4G4F was specifically isolated from RAJI.
31510952	3	83	gly	glycoproteins	571:583	arg1	glycoproteins					571:583	glycoproteins	571:583	glycoproteins	571:583	METHODS Here, we investigated expression patterns of N-linked oligosaccharides of glycoproteins in cells derived from CNS lymphomas and clinical specimens.
31510952	2	84	theme	nervous	458:464	arg1	CNS					474:476	CNS	474:476	CNS	474:476	However, their characterizations have largely been unknown in central nervous system (CNS) lymphoma.
31510952	2	84	theme	nervous	458:464	arg1	system					466:471	central nervous system	450:471	central nervous system (CNS) lymphoma	450:486	However, their characterizations have largely been unknown in central nervous system (CNS) lymphoma.
31510952	0	85	theme	N-linked	27:34	arg1	oligosaccharides					36:51	N-linked oligosaccharides	27:51	N-linked oligosaccharides	27:51	Differential expression of N-linked oligosaccharides in methotrexate-resistant primary central nervous system lymphoma cells.
31510952	1	86	theme	inflammatory	346:357	arg1	responses					359:367	inflammatory responses	346:367	inflammatory responses	346:367	BACKGROUND Oligosaccharides of glycoprotein, particularly negatively-charged sialylated N-glycans, on the surface of lymphomas play important roles in cell-cell interactions and bind immunoglobulin-like lectins, causing inflammatory responses and bioregulation.
31510952	1	87	theme	BACKGROUND	126:135	arg1	Oligosaccharides					137:152	BACKGROUND Oligosaccharides	126:152	BACKGROUND Oligosaccharides of glycoprotein, particularly negatively-charged sialylated N-glycans, on the surface of lymphomas	126:251	BACKGROUND Oligosaccharides of glycoprotein, particularly negatively-charged sialylated N-glycans, on the surface of lymphomas play important roles in cell-cell interactions and bind immunoglobulin-like lectins, causing inflammatory responses and bioregulation.
31510952	6	88	gly	α1,6-fucosylation	1159:1175	arg1	N-glycans					1144:1152	sialylated biantennary galactosylated N-glycans	1106:1152	sialylated biantennary galactosylated N-glycans with α1,6-fucosylation, A2G2F, and A2G2FB from HKBML, TK, and RAJI	1106:1219	We also detected sialylated biantennary galactosylated N-glycans with α1,6-fucosylation, A2G2F, and A2G2FB from HKBML, TK, and RAJI.
31510952	3	89	theme	N-linked	542:549	arg1	oligosaccharides					551:566	N-linked oligosaccharides	542:566	N-linked oligosaccharides of glycoproteins	542:583	METHODS Here, we investigated expression patterns of N-linked oligosaccharides of glycoproteins in cells derived from CNS lymphomas and clinical specimens.
31510952	1	90	theme	important	258:266	arg1	roles					268:272	important roles	258:272	important roles	258:272	BACKGROUND Oligosaccharides of glycoprotein, particularly negatively-charged sialylated N-glycans, on the surface of lymphomas play important roles in cell-cell interactions and bind immunoglobulin-like lectins, causing inflammatory responses and bioregulation.
31510952	0	91	theme	primary	79:85	arg1	cells					119:123	methotrexate-resistant primary central nervous system lymphoma cells	56:123	methotrexate-resistant primary central nervous system lymphoma cells	56:123	Differential expression of N-linked oligosaccharides in methotrexate-resistant primary central nervous system lymphoma cells.
31510952	9	92	theme	complex-type	1426:1437	arg1	oligosaccharides					1439:1454	almost all complex-type oligosaccharides	1415:1454	almost all complex-type oligosaccharides	1415:1454	Interestingly, almost all complex-type oligosaccharides were α2,6-sialylated.
31510952	6	93	from	RAJI	1216:1219	arg1	N-glycans					1144:1152	sialylated biantennary galactosylated N-glycans	1106:1152	sialylated biantennary galactosylated N-glycans with α1,6-fucosylation, A2G2F, and A2G2FB from HKBML, TK, and RAJI	1106:1219	We also detected sialylated biantennary galactosylated N-glycans with α1,6-fucosylation, A2G2F, and A2G2FB from HKBML, TK, and RAJI.
31510952	5	94	theme	MTX-resistant	1069:1081	arg1	RAJI					1083:1086	MTX-resistant RAJI	1069:1086	MTX-resistant RAJI	1069:1086	Major components of the total oligosaccharides were high-mannose type N-glycans, whose level increased in MTX-resistant HKBML and TK but decreased in MTX-resistant RAJI.
31510952	4	95	theme	-resistant	690:699	arg1	cells					701:705	methotrexate (MTX)-resistant cells	672:705	methotrexate (MTX)-resistant cells derived from HKBML and TK as CNS lymphoma, and RAJI as non-CNS lymphoma	672:777	RESULTS We first generated methotrexate (MTX)-resistant cells derived from HKBML and TK as CNS lymphoma, and RAJI as non-CNS lymphoma and determined N-linked oligosaccharide structures in these cells and other non-CNS lymphoma-derived cells including A4/FUK, OYB, and HBL1.
31510952	10	96	theme	resected	1646:1653	arg1	HKBML					1614:1618	HKBML	1614:1618	HKBML	1614:1618	DISCUSSION This is the first study for the expression profile of N-oligosaccharides on MTX-resistant primary CNS lymphoma-derived cells HKBML and TK, and tumor tissues resected from patients with CNS lymphoma, CONCLUSION: These results propose a possibility that the differential expression of high-mannose types and sialylated A2G2F, A2G2FB, and A4G4F on the surface of CNS lymphomas may provide a hint for targets for diagnoses and treatments of the oligosaccharide type-specific lymphomas.
31510952	10	96	theme	resected	1646:1653	arg1	cells					1608:1612	MTX-resistant primary CNS lymphoma-derived cells HKBML and TK	1565:1625	MTX-resistant primary CNS lymphoma-derived cells HKBML and TK	1565:1625	DISCUSSION This is the first study for the expression profile of N-oligosaccharides on MTX-resistant primary CNS lymphoma-derived cells HKBML and TK, and tumor tissues resected from patients with CNS lymphoma, CONCLUSION: These results propose a possibility that the differential expression of high-mannose types and sialylated A2G2F, A2G2FB, and A4G4F on the surface of CNS lymphomas may provide a hint for targets for diagnoses and treatments of the oligosaccharide type-specific lymphomas.
31510952	10	96	theme	resected	1646:1653	arg1	TK					1624:1625	TK	1624:1625	TK	1624:1625	DISCUSSION This is the first study for the expression profile of N-oligosaccharides on MTX-resistant primary CNS lymphoma-derived cells HKBML and TK, and tumor tissues resected from patients with CNS lymphoma, CONCLUSION: These results propose a possibility that the differential expression of high-mannose types and sialylated A2G2F, A2G2FB, and A4G4F on the surface of CNS lymphomas may provide a hint for targets for diagnoses and treatments of the oligosaccharide type-specific lymphomas.
31510952	4	97	theme	lymphoma-derived	863:878	arg1	OYB					904:906	OYB	904:906	OYB	904:906	RESULTS We first generated methotrexate (MTX)-resistant cells derived from HKBML and TK as CNS lymphoma, and RAJI as non-CNS lymphoma and determined N-linked oligosaccharide structures in these cells and other non-CNS lymphoma-derived cells including A4/FUK, OYB, and HBL1.
31510952	4	97	theme	lymphoma-derived	863:878	arg1	HBL1					913:916	HBL1	913:916	HBL1	913:916	RESULTS We first generated methotrexate (MTX)-resistant cells derived from HKBML and TK as CNS lymphoma, and RAJI as non-CNS lymphoma and determined N-linked oligosaccharide structures in these cells and other non-CNS lymphoma-derived cells including A4/FUK, OYB, and HBL1.
31510952	4	97	theme	lymphoma-derived	863:878	arg1	A4/FUK					896:901	A4/FUK	896:901	A4/FUK	896:901	RESULTS We first generated methotrexate (MTX)-resistant cells derived from HKBML and TK as CNS lymphoma, and RAJI as non-CNS lymphoma and determined N-linked oligosaccharide structures in these cells and other non-CNS lymphoma-derived cells including A4/FUK, OYB, and HBL1.
31510952	4	97	theme	lymphoma-derived	863:878	arg1	cells					880:884	other non-CNS lymphoma-derived cells	849:884	other non-CNS lymphoma-derived cells including A4/FUK, OYB, and HBL1	849:916	RESULTS We first generated methotrexate (MTX)-resistant cells derived from HKBML and TK as CNS lymphoma, and RAJI as non-CNS lymphoma and determined N-linked oligosaccharide structures in these cells and other non-CNS lymphoma-derived cells including A4/FUK, OYB, and HBL1.
31510952	6	98	gly	sialylated	1106:1115	arg1	N-glycans					1144:1152	sialylated biantennary galactosylated N-glycans	1106:1152	sialylated biantennary galactosylated N-glycans with α1,6-fucosylation, A2G2F, and A2G2FB from HKBML, TK, and RAJI	1106:1219	We also detected sialylated biantennary galactosylated N-glycans with α1,6-fucosylation, A2G2F, and A2G2FB from HKBML, TK, and RAJI.
31510952	3	99	theme	CNS	607:609	arg1	lymphomas					611:619	CNS lymphomas	607:619	CNS lymphomas	607:619	METHODS Here, we investigated expression patterns of N-linked oligosaccharides of glycoproteins in cells derived from CNS lymphomas and clinical specimens.
31510952	1	100	theme	cell-cell	277:285	arg1	interactions					287:298	cell-cell interactions	277:298	cell-cell interactions	277:298	BACKGROUND Oligosaccharides of glycoprotein, particularly negatively-charged sialylated N-glycans, on the surface of lymphomas play important roles in cell-cell interactions and bind immunoglobulin-like lectins, causing inflammatory responses and bioregulation.
31510952	10	101	theme	lymphomas	1853:1861	arg1	surface					1838:1844	the surface	1834:1844	the surface of CNS lymphomas	1834:1861	DISCUSSION This is the first study for the expression profile of N-oligosaccharides on MTX-resistant primary CNS lymphoma-derived cells HKBML and TK, and tumor tissues resected from patients with CNS lymphoma, CONCLUSION: These results propose a possibility that the differential expression of high-mannose types and sialylated A2G2F, A2G2FB, and A4G4F on the surface of CNS lymphomas may provide a hint for targets for diagnoses and treatments of the oligosaccharide type-specific lymphomas.
29870719	2	0	theme	pathway	481:487	arg1	ALG3					499:502	the N-linked glycosylation pathway component ALG3	454:502	the N-linked glycosylation pathway component ALG3	454:502	Here we report that the Drosophila TSG tumorous imaginal discs (tid), whose phenotypes were previously attributed to mutations in a DnaJ-like chaperone, are in fact driven by the loss of the N-linked glycosylation pathway component ALG3.
29870719	4	1	theme	excess	715:720	arg1	JNK					745:747	JNK	745:747	JNK	745:747	Tumorous growth is driven by inhibited Hippo signaling, induced by excess Jun N-terminal kinase (JNK) activity.
29870719	4	1	theme	excess	715:720	arg1	kinase					737:742	excess Jun N-terminal kinase	715:742	excess Jun N-terminal kinase (JNK) activity	715:757	Tumorous growth is driven by inhibited Hippo signaling, induced by excess Jun N-terminal kinase (JNK) activity.
29870719	2	2	theme	glycosylation	467:479	arg1	ALG3					499:502	the N-linked glycosylation pathway component ALG3	454:502	the N-linked glycosylation pathway component ALG3	454:502	Here we report that the Drosophila TSG tumorous imaginal discs (tid), whose phenotypes were previously attributed to mutations in a DnaJ-like chaperone, are in fact driven by the loss of the N-linked glycosylation pathway component ALG3.
29870719	1	3	theme	tissue	178:183	arg1	growth					185:190	tissue growth	178:190	tissue growth	178:190	Drosophila tumor suppressor genes have revealed molecular pathways that control tissue growth, but mechanisms that regulate mitogenic signaling are far from understood.
29870719	6	4	theme	ligand-receptor	1084:1098	arg1	interactions					1100:1111	ligand-receptor interactions	1084:1111	ligand-receptor interactions	1084:1111	Our results suggest that N-linked glycosylation sets the threshold of TNF receptor signaling by modifying ligand-receptor interactions and that cells may alter this modification to respond appropriately to physiological cues.
29870719	2	5	theme	tumorous	306:313	arg1	discs					324:328	the Drosophila TSG tumorous imaginal discs	287:328	the Drosophila TSG tumorous imaginal discs (tid)	287:334	Here we report that the Drosophila TSG tumorous imaginal discs (tid), whose phenotypes were previously attributed to mutations in a DnaJ-like chaperone, are in fact driven by the loss of the N-linked glycosylation pathway component ALG3.
29870719	2	5	theme	tumorous	306:313	arg1	tid					331:333	tid	331:333	tid	331:333	Here we report that the Drosophila TSG tumorous imaginal discs (tid), whose phenotypes were previously attributed to mutations in a DnaJ-like chaperone, are in fact driven by the loss of the N-linked glycosylation pathway component ALG3.
29870719	2	6	theme	imaginal	315:322	arg1	discs					324:328	the Drosophila TSG tumorous imaginal discs	287:328	the Drosophila TSG tumorous imaginal discs (tid)	287:334	Here we report that the Drosophila TSG tumorous imaginal discs (tid), whose phenotypes were previously attributed to mutations in a DnaJ-like chaperone, are in fact driven by the loss of the N-linked glycosylation pathway component ALG3.
29870719	2	6	theme	imaginal	315:322	arg1	tid					331:333	tid	331:333	tid	331:333	Here we report that the Drosophila TSG tumorous imaginal discs (tid), whose phenotypes were previously attributed to mutations in a DnaJ-like chaperone, are in fact driven by the loss of the N-linked glycosylation pathway component ALG3.
29870719	6	7	theme	physiological	1184:1196	arg1	cues					1198:1201	physiological cues	1184:1201	physiological cues	1184:1201	Our results suggest that N-linked glycosylation sets the threshold of TNF receptor signaling by modifying ligand-receptor interactions and that cells may alter this modification to respond appropriately to physiological cues.
29870719	2	8	theme	TSG	302:304	arg1	discs					324:328	the Drosophila TSG tumorous imaginal discs	287:328	the Drosophila TSG tumorous imaginal discs (tid)	287:334	Here we report that the Drosophila TSG tumorous imaginal discs (tid), whose phenotypes were previously attributed to mutations in a DnaJ-like chaperone, are in fact driven by the loss of the N-linked glycosylation pathway component ALG3.
29870719	2	8	theme	TSG	302:304	arg1	tid					331:333	tid	331:333	tid	331:333	Here we report that the Drosophila TSG tumorous imaginal discs (tid), whose phenotypes were previously attributed to mutations in a DnaJ-like chaperone, are in fact driven by the loss of the N-linked glycosylation pathway component ALG3.
29870719	4	9	theme	kinase	737:742	arg1	activity					750:757	excess Jun N-terminal kinase (JNK) activity	715:757	excess Jun N-terminal kinase (JNK) activity	715:757	Tumorous growth is driven by inhibited Hippo signaling, induced by excess Jun N-terminal kinase (JNK) activity.
29870719	5	10	theme	ectopic	773:779	arg1	activation					785:794	ectopic JNK activation	773:794	ectopic JNK activation	773:794	We show that ectopic JNK activation is caused by aberrant glycosylation of a single protein, the fly tumor necrosis factor (TNF) receptor homolog, which results in increased binding to the continually circulating TNF.
29870719	5	11	theme	receptor	889:896	arg1	protein					844:850	a single protein	835:850	a single protein	835:850	We show that ectopic JNK activation is caused by aberrant glycosylation of a single protein, the fly tumor necrosis factor (TNF) receptor homolog, which results in increased binding to the continually circulating TNF.
29870719	5	11	theme	receptor	889:896	arg1	homolog					898:904	the fly tumor necrosis factor (TNF) receptor homolog	853:904	the fly tumor necrosis factor (TNF) receptor homolog	853:904	We show that ectopic JNK activation is caused by aberrant glycosylation of a single protein, the fly tumor necrosis factor (TNF) receptor homolog, which results in increased binding to the continually circulating TNF.
29870719	5	12	theme	single	837:842	arg1	protein					844:850	a single protein	835:850	a single protein	835:850	We show that ectopic JNK activation is caused by aberrant glycosylation of a single protein, the fly tumor necrosis factor (TNF) receptor homolog, which results in increased binding to the continually circulating TNF.
29870719	5	12	theme	single	837:842	arg1	homolog					898:904	the fly tumor necrosis factor (TNF) receptor homolog	853:904	the fly tumor necrosis factor (TNF) receptor homolog	853:904	We show that ectopic JNK activation is caused by aberrant glycosylation of a single protein, the fly tumor necrosis factor (TNF) receptor homolog, which results in increased binding to the continually circulating TNF.
29870719	4	13	theme	Tumorous	648:655	arg1	growth					657:662	Tumorous growth	648:662	Tumorous growth	648:662	Tumorous growth is driven by inhibited Hippo signaling, induced by excess Jun N-terminal kinase (JNK) activity.
29870719	2	14	theme	Drosophila	291:300	arg1	discs					324:328	the Drosophila TSG tumorous imaginal discs	287:328	the Drosophila TSG tumorous imaginal discs (tid)	287:334	Here we report that the Drosophila TSG tumorous imaginal discs (tid), whose phenotypes were previously attributed to mutations in a DnaJ-like chaperone, are in fact driven by the loss of the N-linked glycosylation pathway component ALG3.
29870719	2	14	theme	Drosophila	291:300	arg1	tid					331:333	tid	331:333	tid	331:333	Here we report that the Drosophila TSG tumorous imaginal discs (tid), whose phenotypes were previously attributed to mutations in a DnaJ-like chaperone, are in fact driven by the loss of the N-linked glycosylation pathway component ALG3.
29870719	6	15	theme	receptor	1052:1059	arg1	signaling					1061:1069	TNF receptor signaling	1048:1069	TNF receptor signaling	1048:1069	Our results suggest that N-linked glycosylation sets the threshold of TNF receptor signaling by modifying ligand-receptor interactions and that cells may alter this modification to respond appropriately to physiological cues.
29870719	2	16	theme	DnaJ-like	399:407	arg1	chaperone					409:417	a DnaJ-like chaperone	397:417	a DnaJ-like chaperone	397:417	Here we report that the Drosophila TSG tumorous imaginal discs (tid), whose phenotypes were previously attributed to mutations in a DnaJ-like chaperone, are in fact driven by the loss of the N-linked glycosylation pathway component ALG3.
29870719	5	17	theme	JNK	781:783	arg1	activation					785:794	ectopic JNK activation	773:794	ectopic JNK activation	773:794	We show that ectopic JNK activation is caused by aberrant glycosylation of a single protein, the fly tumor necrosis factor (TNF) receptor homolog, which results in increased binding to the continually circulating TNF.
29870719	3	18	theme	tissue	537:542	arg1	growth					544:549	tissue growth	537:549	tissue growth	537:549	tid/alg3 imaginal discs display tissue growth and architecture defects that share characteristics of both neoplastic and hyperplastic mutants.
29870719	5	19	theme	fly	857:859	arg1	TNF					884:886	TNF	884:886	TNF	884:886	We show that ectopic JNK activation is caused by aberrant glycosylation of a single protein, the fly tumor necrosis factor (TNF) receptor homolog, which results in increased binding to the continually circulating TNF.
29870719	5	19	theme	fly	857:859	arg1	factor					876:881	fly tumor necrosis factor	857:881	the fly tumor necrosis factor (TNF) receptor homolog	853:904	We show that ectopic JNK activation is caused by aberrant glycosylation of a single protein, the fly tumor necrosis factor (TNF) receptor homolog, which results in increased binding to the continually circulating TNF.
29870719	0	20	theme	Drosophila	2:11	arg1	Gene					30:33	A Drosophila Tumor Suppressor Gene	0:33	A Drosophila Tumor Suppressor Gene	0:33	A Drosophila Tumor Suppressor Gene Prevents Tonic TNF Signaling through Receptor N-Glycosylation.
29870719	0	21	theme	Receptor	72:79	arg1	N-Glycosylation					81:95	Receptor N-Glycosylation	72:95	Receptor N-Glycosylation	72:95	A Drosophila Tumor Suppressor Gene Prevents Tonic TNF Signaling through Receptor N-Glycosylation.
29870719	5	22	theme	increased	924:932	arg1	binding					934:940	increased binding	924:940	increased binding to the continually circulating TNF	924:975	We show that ectopic JNK activation is caused by aberrant glycosylation of a single protein, the fly tumor necrosis factor (TNF) receptor homolog, which results in increased binding to the continually circulating TNF.
29870719	5	23	theme	tumor	861:865	arg1	TNF					884:886	TNF	884:886	TNF	884:886	We show that ectopic JNK activation is caused by aberrant glycosylation of a single protein, the fly tumor necrosis factor (TNF) receptor homolog, which results in increased binding to the continually circulating TNF.
29870719	5	23	theme	tumor	861:865	arg1	factor					876:881	fly tumor necrosis factor	857:881	the fly tumor necrosis factor (TNF) receptor homolog	853:904	We show that ectopic JNK activation is caused by aberrant glycosylation of a single protein, the fly tumor necrosis factor (TNF) receptor homolog, which results in increased binding to the continually circulating TNF.
29870719	5	24	theme	aberrant	809:816	arg1	glycosylation					818:830	aberrant glycosylation	809:830	aberrant glycosylation of a single protein, the fly tumor necrosis factor (TNF) receptor homolog, which results in increased binding to the continually circulating TNF	809:975	We show that ectopic JNK activation is caused by aberrant glycosylation of a single protein, the fly tumor necrosis factor (TNF) receptor homolog, which results in increased binding to the continually circulating TNF.
29870719	2	25	link	N-linked	458:465	arg1	ALG3					499:502	the N-linked glycosylation pathway component ALG3	454:502	the N-linked glycosylation pathway component ALG3	454:502	Here we report that the Drosophila TSG tumorous imaginal discs (tid), whose phenotypes were previously attributed to mutations in a DnaJ-like chaperone, are in fact driven by the loss of the N-linked glycosylation pathway component ALG3.
29870719	1	26	theme	Drosophila	98:107	arg1	genes					126:130	Drosophila tumor suppressor genes	98:130	Drosophila tumor suppressor genes	98:130	Drosophila tumor suppressor genes have revealed molecular pathways that control tissue growth, but mechanisms that regulate mitogenic signaling are far from understood.
29870719	4	27	theme	Hippo	687:691	arg1	signaling					693:701	inhibited Hippo signaling	677:701	inhibited Hippo signaling	677:701	Tumorous growth is driven by inhibited Hippo signaling, induced by excess Jun N-terminal kinase (JNK) activity.
29870719	0	28	theme	Suppressor	19:28	arg1	Gene					30:33	A Drosophila Tumor Suppressor Gene	0:33	A Drosophila Tumor Suppressor Gene	0:33	A Drosophila Tumor Suppressor Gene Prevents Tonic TNF Signaling through Receptor N-Glycosylation.
29870719	1	29	theme	tumor	109:113	arg1	genes					126:130	Drosophila tumor suppressor genes	98:130	Drosophila tumor suppressor genes	98:130	Drosophila tumor suppressor genes have revealed molecular pathways that control tissue growth, but mechanisms that regulate mitogenic signaling are far from understood.
29870719	0	30	theme	Tumor	13:17	arg1	Gene					30:33	A Drosophila Tumor Suppressor Gene	0:33	A Drosophila Tumor Suppressor Gene	0:33	A Drosophila Tumor Suppressor Gene Prevents Tonic TNF Signaling through Receptor N-Glycosylation.
29870719	1	31	theme	suppressor	115:124	arg1	genes					126:130	Drosophila tumor suppressor genes	98:130	Drosophila tumor suppressor genes	98:130	Drosophila tumor suppressor genes have revealed molecular pathways that control tissue growth, but mechanisms that regulate mitogenic signaling are far from understood.
29870719	1	32	theme	mitogenic	222:230	arg1	signaling					232:240	mitogenic signaling	222:240	mitogenic signaling	222:240	Drosophila tumor suppressor genes have revealed molecular pathways that control tissue growth, but mechanisms that regulate mitogenic signaling are far from understood.
29870719	2	33	theme	N-linked	458:465	arg1	ALG3					499:502	the N-linked glycosylation pathway component ALG3	454:502	the N-linked glycosylation pathway component ALG3	454:502	Here we report that the Drosophila TSG tumorous imaginal discs (tid), whose phenotypes were previously attributed to mutations in a DnaJ-like chaperone, are in fact driven by the loss of the N-linked glycosylation pathway component ALG3.
29870719	5	34	theme	necrosis	867:874	arg1	TNF					884:886	TNF	884:886	TNF	884:886	We show that ectopic JNK activation is caused by aberrant glycosylation of a single protein, the fly tumor necrosis factor (TNF) receptor homolog, which results in increased binding to the continually circulating TNF.
29870719	5	34	theme	necrosis	867:874	arg1	factor					876:881	fly tumor necrosis factor	857:881	the fly tumor necrosis factor (TNF) receptor homolog	853:904	We show that ectopic JNK activation is caused by aberrant glycosylation of a single protein, the fly tumor necrosis factor (TNF) receptor homolog, which results in increased binding to the continually circulating TNF.
29870719	5	35	theme	protein	844:850	arg1	glycosylation					818:830	aberrant glycosylation	809:830	aberrant glycosylation of a single protein, the fly tumor necrosis factor (TNF) receptor homolog, which results in increased binding to the continually circulating TNF	809:975	We show that ectopic JNK activation is caused by aberrant glycosylation of a single protein, the fly tumor necrosis factor (TNF) receptor homolog, which results in increased binding to the continually circulating TNF.
29870719	4	36	theme	inhibited	677:685	arg1	signaling					693:701	inhibited Hippo signaling	677:701	inhibited Hippo signaling	677:701	Tumorous growth is driven by inhibited Hippo signaling, induced by excess Jun N-terminal kinase (JNK) activity.
29870719	3	37	theme	neoplastic	611:620	arg1	mutants					639:645	both neoplastic and hyperplastic mutants	606:645	both neoplastic and hyperplastic mutants	606:645	tid/alg3 imaginal discs display tissue growth and architecture defects that share characteristics of both neoplastic and hyperplastic mutants.
29870719	0	38	theme	TNF	50:52	arg1	Signaling					54:62	Tonic TNF Signaling	44:62	Tonic TNF Signaling	44:62	A Drosophila Tumor Suppressor Gene Prevents Tonic TNF Signaling through Receptor N-Glycosylation.
29870719	5	39	theme	factor	876:881	arg1	protein					844:850	a single protein	835:850	a single protein	835:850	We show that ectopic JNK activation is caused by aberrant glycosylation of a single protein, the fly tumor necrosis factor (TNF) receptor homolog, which results in increased binding to the continually circulating TNF.
29870719	5	39	theme	factor	876:881	arg1	homolog					898:904	the fly tumor necrosis factor (TNF) receptor homolog	853:904	the fly tumor necrosis factor (TNF) receptor homolog	853:904	We show that ectopic JNK activation is caused by aberrant glycosylation of a single protein, the fly tumor necrosis factor (TNF) receptor homolog, which results in increased binding to the continually circulating TNF.
29870719	2	40	from	mutations	384:392	arg1	chaperone					409:417	a DnaJ-like chaperone	397:417	a DnaJ-like chaperone	397:417	Here we report that the Drosophila TSG tumorous imaginal discs (tid), whose phenotypes were previously attributed to mutations in a DnaJ-like chaperone, are in fact driven by the loss of the N-linked glycosylation pathway component ALG3.
29870719	4	41	theme	N-terminal	726:735	arg1	JNK					745:747	JNK	745:747	JNK	745:747	Tumorous growth is driven by inhibited Hippo signaling, induced by excess Jun N-terminal kinase (JNK) activity.
29870719	4	41	theme	N-terminal	726:735	arg1	kinase					737:742	excess Jun N-terminal kinase	715:742	excess Jun N-terminal kinase (JNK) activity	715:757	Tumorous growth is driven by inhibited Hippo signaling, induced by excess Jun N-terminal kinase (JNK) activity.
29870719	0	42	theme	Tonic	44:48	arg1	Signaling					54:62	Tonic TNF Signaling	44:62	Tonic TNF Signaling	44:62	A Drosophila Tumor Suppressor Gene Prevents Tonic TNF Signaling through Receptor N-Glycosylation.
29870719	6	43	theme	TNF	1048:1050	arg1	signaling					1061:1069	TNF receptor signaling	1048:1069	TNF receptor signaling	1048:1069	Our results suggest that N-linked glycosylation sets the threshold of TNF receptor signaling by modifying ligand-receptor interactions and that cells may alter this modification to respond appropriately to physiological cues.
29870719	5	44	gly	glycosylation	818:830	arg1	protein					844:850	a single protein	835:850	a single protein	835:850	We show that ectopic JNK activation is caused by aberrant glycosylation of a single protein, the fly tumor necrosis factor (TNF) receptor homolog, which results in increased binding to the continually circulating TNF.
29870719	5	44	gly	glycosylation	818:830	arg1	homolog					898:904	the fly tumor necrosis factor (TNF) receptor homolog	853:904	the fly tumor necrosis factor (TNF) receptor homolog	853:904	We show that ectopic JNK activation is caused by aberrant glycosylation of a single protein, the fly tumor necrosis factor (TNF) receptor homolog, which results in increased binding to the continually circulating TNF.
29870719	3	45	theme	tid/alg3	505:512	arg1	discs					523:527	tid/alg3 imaginal discs	505:527	tid/alg3 imaginal discs	505:527	tid/alg3 imaginal discs display tissue growth and architecture defects that share characteristics of both neoplastic and hyperplastic mutants.
29870719	4	46	theme	Jun	722:724	arg1	JNK					745:747	JNK	745:747	JNK	745:747	Tumorous growth is driven by inhibited Hippo signaling, induced by excess Jun N-terminal kinase (JNK) activity.
29870719	4	46	theme	Jun	722:724	arg1	kinase					737:742	excess Jun N-terminal kinase	715:742	excess Jun N-terminal kinase (JNK) activity	715:757	Tumorous growth is driven by inhibited Hippo signaling, induced by excess Jun N-terminal kinase (JNK) activity.
29870719	3	47	theme	mutants	639:645	arg1	characteristics					587:601	characteristics	587:601	characteristics of both neoplastic and hyperplastic mutants	587:645	tid/alg3 imaginal discs display tissue growth and architecture defects that share characteristics of both neoplastic and hyperplastic mutants.
29870719	3	48	theme	architecture	555:566	arg1	defects					568:574	tissue growth and architecture defects	537:574	tissue growth and architecture defects that share characteristics of both neoplastic and hyperplastic mutants	537:645	tid/alg3 imaginal discs display tissue growth and architecture defects that share characteristics of both neoplastic and hyperplastic mutants.
29870719	6	49	link	N-linked	1003:1010	arg1	glycosylation					1012:1024	N-linked glycosylation	1003:1024	N-linked glycosylation	1003:1024	Our results suggest that N-linked glycosylation sets the threshold of TNF receptor signaling by modifying ligand-receptor interactions and that cells may alter this modification to respond appropriately to physiological cues.
29870719	5	50	theme	circulating	961:971	arg1	TNF					973:975	the continually circulating TNF	945:975	the continually circulating TNF	945:975	We show that ectopic JNK activation is caused by aberrant glycosylation of a single protein, the fly tumor necrosis factor (TNF) receptor homolog, which results in increased binding to the continually circulating TNF.
29870719	3	51	theme	growth	544:549	arg1	defects					568:574	tissue growth and architecture defects	537:574	tissue growth and architecture defects that share characteristics of both neoplastic and hyperplastic mutants	537:645	tid/alg3 imaginal discs display tissue growth and architecture defects that share characteristics of both neoplastic and hyperplastic mutants.
29870719	1	52	theme	molecular	146:154	arg1	pathways					156:163	molecular pathways	146:163	molecular pathways that control tissue growth	146:190	Drosophila tumor suppressor genes have revealed molecular pathways that control tissue growth, but mechanisms that regulate mitogenic signaling are far from understood.
29870719	6	53	theme	N-linked	1003:1010	arg1	glycosylation					1012:1024	N-linked glycosylation	1003:1024	N-linked glycosylation	1003:1024	Our results suggest that N-linked glycosylation sets the threshold of TNF receptor signaling by modifying ligand-receptor interactions and that cells may alter this modification to respond appropriately to physiological cues.
29870719	2	54	theme	ALG3	499:502	arg1	loss					446:449	the loss	442:449	the loss of the N-linked glycosylation pathway component ALG3	442:502	Here we report that the Drosophila TSG tumorous imaginal discs (tid), whose phenotypes were previously attributed to mutations in a DnaJ-like chaperone, are in fact driven by the loss of the N-linked glycosylation pathway component ALG3.
29870719	3	55	theme	imaginal	514:521	arg1	discs					523:527	tid/alg3 imaginal discs	505:527	tid/alg3 imaginal discs	505:527	tid/alg3 imaginal discs display tissue growth and architecture defects that share characteristics of both neoplastic and hyperplastic mutants.
29870719	6	56	theme	signaling	1061:1069	arg1	threshold					1035:1043	the threshold	1031:1043	the threshold of TNF receptor signaling	1031:1069	Our results suggest that N-linked glycosylation sets the threshold of TNF receptor signaling by modifying ligand-receptor interactions and that cells may alter this modification to respond appropriately to physiological cues.
29870719	2	57	theme	component	489:497	arg1	ALG3					499:502	the N-linked glycosylation pathway component ALG3	454:502	the N-linked glycosylation pathway component ALG3	454:502	Here we report that the Drosophila TSG tumorous imaginal discs (tid), whose phenotypes were previously attributed to mutations in a DnaJ-like chaperone, are in fact driven by the loss of the N-linked glycosylation pathway component ALG3.
29870719	3	58	theme	hyperplastic	626:637	arg1	mutants					639:645	both neoplastic and hyperplastic mutants	606:645	both neoplastic and hyperplastic mutants	606:645	tid/alg3 imaginal discs display tissue growth and architecture defects that share characteristics of both neoplastic and hyperplastic mutants.
31077497	4	0	from	21 days	852:858	arg1	ileum					843:847	the ileum	839:847	the ileum at 21 days of age and Escherichia-Shigella in the jejunum at 28 days of age	839:923	The results showed that IUGR piglets had a decreased alpha diversity of jejunum microbiota at 7 and 21 days of age; had lower abundances of Bacteroidetes and Bacteroides in the jejunum at 7, 21 and 28 days of age, Oscillibacter in the jejunum at 21 days of age, and Firmicutes in the ileum at 21 days of age; whereas they had higher abundances of Proteobacteria and Pasteurella in the ileum at 21 days of age and Escherichia-Shigella in the jejunum at 28 days of age.
31077497	4	1	from	ileum	843:847	arg1	28 days					910:916	28 days	910:916	28 days of age	910:923	The results showed that IUGR piglets had a decreased alpha diversity of jejunum microbiota at 7 and 21 days of age; had lower abundances of Bacteroidetes and Bacteroides in the jejunum at 7, 21 and 28 days of age, Oscillibacter in the jejunum at 21 days of age, and Firmicutes in the ileum at 21 days of age; whereas they had higher abundances of Proteobacteria and Pasteurella in the ileum at 21 days of age and Escherichia-Shigella in the jejunum at 28 days of age.
31077497	4	1	from	ileum	843:847	arg1	jejunum					899:905	the jejunum	895:905	the jejunum at 28 days of age	895:923	The results showed that IUGR piglets had a decreased alpha diversity of jejunum microbiota at 7 and 21 days of age; had lower abundances of Bacteroidetes and Bacteroides in the jejunum at 7, 21 and 28 days of age, Oscillibacter in the jejunum at 21 days of age, and Firmicutes in the ileum at 21 days of age; whereas they had higher abundances of Proteobacteria and Pasteurella in the ileum at 21 days of age and Escherichia-Shigella in the jejunum at 28 days of age.
31077497	4	2	theme	Bacteroides	616:626	arg1	abundances					584:593	lower abundances	578:593	lower abundances of Bacteroidetes and Bacteroides in the jejunum at 7, 21 and 28 days of age, Oscillibacter in the jejunum at 21 days of age, and Firmicutes in the ileum	578:746	The results showed that IUGR piglets had a decreased alpha diversity of jejunum microbiota at 7 and 21 days of age; had lower abundances of Bacteroidetes and Bacteroides in the jejunum at 7, 21 and 28 days of age, Oscillibacter in the jejunum at 21 days of age, and Firmicutes in the ileum at 21 days of age; whereas they had higher abundances of Proteobacteria and Pasteurella in the ileum at 21 days of age and Escherichia-Shigella in the jejunum at 28 days of age.
31077497	3	3	dep	METHODS	306:312	arg1	identified					329:338	identified	329:338	identified the alterations of small intestinal microbiota in IUGR piglets on 7, 21 and 28 days of age using 16S rRNA sequencing	329:455	METHODS AND RESULTS We identified the alterations of small intestinal microbiota in IUGR piglets on 7, 21 and 28 days of age using 16S rRNA sequencing.
31077497	4	4	theme	age	762:764	arg1	21 days					751:757	21 days	751:757	21 days of age	751:764	The results showed that IUGR piglets had a decreased alpha diversity of jejunum microbiota at 7 and 21 days of age; had lower abundances of Bacteroidetes and Bacteroides in the jejunum at 7, 21 and 28 days of age, Oscillibacter in the jejunum at 21 days of age, and Firmicutes in the ileum at 21 days of age; whereas they had higher abundances of Proteobacteria and Pasteurella in the ileum at 21 days of age and Escherichia-Shigella in the jejunum at 28 days of age.
31077497	6	5	theme	carbohydrate	1297:1308	arg1	metabolism					1310:1319	microbiota-associated carbohydrate metabolism	1275:1319	microbiota-associated carbohydrate metabolism	1275:1319	Gene function prediction analysis indicated that microbiota-associated carbohydrate metabolism, lipid metabolism, glycan biosynthesis and metabolism, amino acid metabolism, and xenobiotics biodegradation and metabolism were downregulated in the IUGR piglets compared to control piglets.
31077497	2	6	theme	IUGR	292:295	arg1	piglets					297:303	IUGR piglets	292:303	IUGR piglets	292:303	The present study was conducted to unveil the alterations in intestinal microbiota in IUGR piglets.
31077497	4	7	from	abundances	584:593	arg1	jejunum					693:699	the jejunum	689:699	the jejunum at 21 days of age	689:717	The results showed that IUGR piglets had a decreased alpha diversity of jejunum microbiota at 7 and 21 days of age; had lower abundances of Bacteroidetes and Bacteroides in the jejunum at 7, 21 and 28 days of age, Oscillibacter in the jejunum at 21 days of age, and Firmicutes in the ileum at 21 days of age; whereas they had higher abundances of Proteobacteria and Pasteurella in the ileum at 21 days of age and Escherichia-Shigella in the jejunum at 28 days of age.
31077497	4	7	from	abundances	584:593	arg1	jejunum					635:641	the jejunum	631:641	the jejunum at 7	631:646	The results showed that IUGR piglets had a decreased alpha diversity of jejunum microbiota at 7 and 21 days of age; had lower abundances of Bacteroidetes and Bacteroides in the jejunum at 7, 21 and 28 days of age, Oscillibacter in the jejunum at 21 days of age, and Firmicutes in the ileum at 21 days of age; whereas they had higher abundances of Proteobacteria and Pasteurella in the ileum at 21 days of age and Escherichia-Shigella in the jejunum at 28 days of age.
31077497	4	7	from	abundances	584:593	arg1	ileum					742:746	the ileum	738:746	the ileum	738:746	The results showed that IUGR piglets had a decreased alpha diversity of jejunum microbiota at 7 and 21 days of age; had lower abundances of Bacteroidetes and Bacteroides in the jejunum at 7, 21 and 28 days of age, Oscillibacter in the jejunum at 21 days of age, and Firmicutes in the ileum at 21 days of age; whereas they had higher abundances of Proteobacteria and Pasteurella in the ileum at 21 days of age and Escherichia-Shigella in the jejunum at 28 days of age.
31077497	7	8	theme	IUGR	1623:1626	arg1	piglets					1628:1634	the newborn IUGR piglets	1611:1634	the newborn IUGR piglets	1611:1634	CONCLUSIONS The present study profiled the intestinal microbiota of newborn piglets with IUGR and the newborn IUGR piglets have lower diversity and different taxonomic abundances.
31077497	4	9	theme	Bacteroidetes	598:610	arg1	abundances					584:593	lower abundances	578:593	lower abundances of Bacteroidetes and Bacteroides in the jejunum at 7, 21 and 28 days of age, Oscillibacter in the jejunum at 21 days of age, and Firmicutes in the ileum	578:746	The results showed that IUGR piglets had a decreased alpha diversity of jejunum microbiota at 7 and 21 days of age; had lower abundances of Bacteroidetes and Bacteroides in the jejunum at 7, 21 and 28 days of age, Oscillibacter in the jejunum at 21 days of age, and Firmicutes in the ileum at 21 days of age; whereas they had higher abundances of Proteobacteria and Pasteurella in the ileum at 21 days of age and Escherichia-Shigella in the jejunum at 28 days of age.
31077497	8	10	theme	IUGR	1940:1943	arg1	development					1925:1935	development	1925:1935	development	1925:1935	Alterations in the abundances of Bacteroidetes, Bacteroides, Proteobacteria Escherichia-Shigella and Pasteurella may be involved in nutrient digestion and absorption, as well as the potential mechanisms connecting to the growth and development of IUGR in mammals.
31077497	8	10	theme	IUGR	1940:1943	arg1	growth					1914:1919	growth	1914:1919	growth	1914:1919	Alterations in the abundances of Bacteroidetes, Bacteroides, Proteobacteria Escherichia-Shigella and Pasteurella may be involved in nutrient digestion and absorption, as well as the potential mechanisms connecting to the growth and development of IUGR in mammals.
31077497	4	11	from	ileum	742:746	arg1	abundances					584:593	lower abundances	578:593	lower abundances of Bacteroidetes and Bacteroides in the jejunum at 7, 21 and 28 days of age, Oscillibacter in the jejunum at 21 days of age, and Firmicutes in the ileum	578:746	The results showed that IUGR piglets had a decreased alpha diversity of jejunum microbiota at 7 and 21 days of age; had lower abundances of Bacteroidetes and Bacteroides in the jejunum at 7, 21 and 28 days of age, Oscillibacter in the jejunum at 21 days of age, and Firmicutes in the ileum at 21 days of age; whereas they had higher abundances of Proteobacteria and Pasteurella in the ileum at 21 days of age and Escherichia-Shigella in the jejunum at 28 days of age.
31077497	9	12	theme	IUGR	2199:2202	arg1	treatment					2204:2212	IUGR treatment	2199:2212	IUGR treatment	2199:2212	SIGNIFICANCE AND IMPACT OF THE STUDY The small intestinal microbiota were highly shaped in the IUGR piglets, which might further mediate the growth and development of IUGR piglets; and the gut microbiota could serve as a potential target for IUGR treatment.
31077497	2	13	theme	present	210:216	arg1	study					218:222	The present study	206:222	The present study	206:222	The present study was conducted to unveil the alterations in intestinal microbiota in IUGR piglets.
31077497	8	14	theme	Pasteurella	1794:1804	arg1	abundances					1712:1721	the abundances	1708:1721	the abundances of Bacteroidetes, Bacteroides, Proteobacteria Escherichia-Shigella and Pasteurella	1708:1804	Alterations in the abundances of Bacteroidetes, Bacteroides, Proteobacteria Escherichia-Shigella and Pasteurella may be involved in nutrient digestion and absorption, as well as the potential mechanisms connecting to the growth and development of IUGR in mammals.
31077497	1	15	theme	AIM	128:130	arg1	problem					186:192	a prevalent problem	174:192	a prevalent problem in mammals	174:203	AIM Intrauterine growth retardation (IUGR) is a prevalent problem in mammals.
31077497	1	15	theme	AIM	128:130	arg1	IUGR					165:168	IUGR	165:168	IUGR	165:168	AIM Intrauterine growth retardation (IUGR) is a prevalent problem in mammals.
31077497	1	15	theme	AIM	128:130	arg1	retardation					152:162	AIM Intrauterine growth retardation	128:162	AIM Intrauterine growth retardation (IUGR)	128:169	AIM Intrauterine growth retardation (IUGR) is a prevalent problem in mammals.
31077497	9	16	theme	STUDY	1988:1992	arg1	IMPACT					1974:1979	IMPACT	1974:1979	IMPACT OF THE STUDY	1974:1992	SIGNIFICANCE AND IMPACT OF THE STUDY The small intestinal microbiota were highly shaped in the IUGR piglets, which might further mediate the growth and development of IUGR piglets; and the gut microbiota could serve as a potential target for IUGR treatment.
31077497	9	16	theme	STUDY	1988:1992	arg1	SIGNIFICANCE					1957:1968	SIGNIFICANCE	1957:1968	SIGNIFICANCE	1957:1968	SIGNIFICANCE AND IMPACT OF THE STUDY The small intestinal microbiota were highly shaped in the IUGR piglets, which might further mediate the growth and development of IUGR piglets; and the gut microbiota could serve as a potential target for IUGR treatment.
31077497	5	17	theme	piglets	1217:1223	arg1	BW					1206:1207	the BW	1202:1207	the BW of IUGR piglets	1202:1223	Correlation analysis showed that Bacteroides, Oscillibacter and Ruminococcaceae_UCG-002 compositions were positively associated with the body weight (BW) of IUGR piglets, nevertheless Proteobacteria and Escherichia-Shigella relative abundances were negatively correlated with the BW of IUGR piglets.
31077497	6	18	theme	function	1231:1238	arg1	analysis					1251:1258	Gene function prediction analysis	1226:1258	Gene function prediction analysis	1226:1258	Gene function prediction analysis indicated that microbiota-associated carbohydrate metabolism, lipid metabolism, glycan biosynthesis and metabolism, amino acid metabolism, and xenobiotics biodegradation and metabolism were downregulated in the IUGR piglets compared to control piglets.
31077497	9	19	dep	SIGNIFICANCE	1957:1968	arg1	microbiota					2015:2024	The small intestinal microbiota	1994:2024	The small intestinal microbiota	1994:2024	SIGNIFICANCE AND IMPACT OF THE STUDY The small intestinal microbiota were highly shaped in the IUGR piglets, which might further mediate the growth and development of IUGR piglets; and the gut microbiota could serve as a potential target for IUGR treatment.
31077497	1	20	theme	growth	145:150	arg1	problem					186:192	a prevalent problem	174:192	a prevalent problem in mammals	174:203	AIM Intrauterine growth retardation (IUGR) is a prevalent problem in mammals.
31077497	1	20	theme	growth	145:150	arg1	IUGR					165:168	IUGR	165:168	IUGR	165:168	AIM Intrauterine growth retardation (IUGR) is a prevalent problem in mammals.
31077497	1	20	theme	growth	145:150	arg1	retardation					152:162	AIM Intrauterine growth retardation	128:162	AIM Intrauterine growth retardation (IUGR)	128:169	AIM Intrauterine growth retardation (IUGR) is a prevalent problem in mammals.
31077497	7	21	theme	piglets	1589:1595	arg1	microbiota					1567:1576	the intestinal microbiota	1552:1576	the intestinal microbiota of newborn piglets with IUGR and the newborn IUGR piglets	1552:1634	CONCLUSIONS The present study profiled the intestinal microbiota of newborn piglets with IUGR and the newborn IUGR piglets have lower diversity and different taxonomic abundances.
31077497	9	22	theme	small	1998:2002	arg1	microbiota					2015:2024	The small intestinal microbiota	1994:2024	The small intestinal microbiota	1994:2024	SIGNIFICANCE AND IMPACT OF THE STUDY The small intestinal microbiota were highly shaped in the IUGR piglets, which might further mediate the growth and development of IUGR piglets; and the gut microbiota could serve as a potential target for IUGR treatment.
31077497	0	23	theme	lower	76:80	arg1	diversity					82:90	lower diversity	76:90	lower diversity	76:90	Gut microbiota of newborn piglets with intrauterine growth restriction have lower diversity and different taxonomic abundances.
31077497	5	24	theme	body	1063:1066	arg1	BW					1076:1077	BW	1076:1077	BW	1076:1077	Correlation analysis showed that Bacteroides, Oscillibacter and Ruminococcaceae_UCG-002 compositions were positively associated with the body weight (BW) of IUGR piglets, nevertheless Proteobacteria and Escherichia-Shigella relative abundances were negatively correlated with the BW of IUGR piglets.
31077497	5	24	theme	body	1063:1066	arg1	weight					1068:1073	the body weight	1059:1073	the body weight (BW) of IUGR piglets	1059:1094	Correlation analysis showed that Bacteroides, Oscillibacter and Ruminococcaceae_UCG-002 compositions were positively associated with the body weight (BW) of IUGR piglets, nevertheless Proteobacteria and Escherichia-Shigella relative abundances were negatively correlated with the BW of IUGR piglets.
31077497	4	25	from	21 days	704:710	arg1	jejunum					693:699	the jejunum	689:699	the jejunum at 21 days of age	689:717	The results showed that IUGR piglets had a decreased alpha diversity of jejunum microbiota at 7 and 21 days of age; had lower abundances of Bacteroidetes and Bacteroides in the jejunum at 7, 21 and 28 days of age, Oscillibacter in the jejunum at 21 days of age, and Firmicutes in the ileum at 21 days of age; whereas they had higher abundances of Proteobacteria and Pasteurella in the ileum at 21 days of age and Escherichia-Shigella in the jejunum at 28 days of age.
31077497	4	25	from	21 days	704:710	arg1	28 days					656:662	28 days	656:662	28 days	656:662	The results showed that IUGR piglets had a decreased alpha diversity of jejunum microbiota at 7 and 21 days of age; had lower abundances of Bacteroidetes and Bacteroides in the jejunum at 7, 21 and 28 days of age, Oscillibacter in the jejunum at 21 days of age, and Firmicutes in the ileum at 21 days of age; whereas they had higher abundances of Proteobacteria and Pasteurella in the ileum at 21 days of age and Escherichia-Shigella in the jejunum at 28 days of age.
31077497	7	26	theme	intestinal	1556:1565	arg1	microbiota					1567:1576	the intestinal microbiota	1552:1576	the intestinal microbiota of newborn piglets with IUGR and the newborn IUGR piglets	1552:1634	CONCLUSIONS The present study profiled the intestinal microbiota of newborn piglets with IUGR and the newborn IUGR piglets have lower diversity and different taxonomic abundances.
31077497	4	27	theme	IUGR	482:485	arg1	piglets					487:493	IUGR piglets	482:493	IUGR piglets	482:493	The results showed that IUGR piglets had a decreased alpha diversity of jejunum microbiota at 7 and 21 days of age; had lower abundances of Bacteroidetes and Bacteroides in the jejunum at 7, 21 and 28 days of age, Oscillibacter in the jejunum at 21 days of age, and Firmicutes in the ileum at 21 days of age; whereas they had higher abundances of Proteobacteria and Pasteurella in the ileum at 21 days of age and Escherichia-Shigella in the jejunum at 28 days of age.
31077497	4	28	from	jejunum	693:699	arg1	abundances					584:593	lower abundances	578:593	lower abundances of Bacteroidetes and Bacteroides in the jejunum at 7, 21 and 28 days of age, Oscillibacter in the jejunum at 21 days of age, and Firmicutes in the ileum	578:746	The results showed that IUGR piglets had a decreased alpha diversity of jejunum microbiota at 7 and 21 days of age; had lower abundances of Bacteroidetes and Bacteroides in the jejunum at 7, 21 and 28 days of age, Oscillibacter in the jejunum at 21 days of age, and Firmicutes in the ileum at 21 days of age; whereas they had higher abundances of Proteobacteria and Pasteurella in the ileum at 21 days of age and Escherichia-Shigella in the jejunum at 28 days of age.
31077497	2	29	from	alterations	252:262	arg1	microbiota					278:287	intestinal microbiota	267:287	intestinal microbiota in IUGR piglets	267:303	The present study was conducted to unveil the alterations in intestinal microbiota in IUGR piglets.
31077497	0	30	theme	taxonomic	106:114	arg1	abundances					116:125	different taxonomic abundances	96:125	different taxonomic abundances	96:125	Gut microbiota of newborn piglets with intrauterine growth restriction have lower diversity and different taxonomic abundances.
31077497	7	31	theme	taxonomic	1671:1679	arg1	abundances					1681:1690	different taxonomic abundances	1661:1690	different taxonomic abundances	1661:1690	CONCLUSIONS The present study profiled the intestinal microbiota of newborn piglets with IUGR and the newborn IUGR piglets have lower diversity and different taxonomic abundances.
31077497	4	32	theme	age	921:923	arg1	28 days					910:916	28 days	910:916	28 days of age	910:923	The results showed that IUGR piglets had a decreased alpha diversity of jejunum microbiota at 7 and 21 days of age; had lower abundances of Bacteroidetes and Bacteroides in the jejunum at 7, 21 and 28 days of age, Oscillibacter in the jejunum at 21 days of age, and Firmicutes in the ileum at 21 days of age; whereas they had higher abundances of Proteobacteria and Pasteurella in the ileum at 21 days of age and Escherichia-Shigella in the jejunum at 28 days of age.
31077497	9	33	theme	IUGR	2052:2055	arg1	piglets					2057:2063	the IUGR piglets	2048:2063	the IUGR piglets	2048:2063	SIGNIFICANCE AND IMPACT OF THE STUDY The small intestinal microbiota were highly shaped in the IUGR piglets, which might further mediate the growth and development of IUGR piglets; and the gut microbiota could serve as a potential target for IUGR treatment.
31077497	8	34	from	Alterations	1693:1703	arg1	abundances					1712:1721	the abundances	1708:1721	the abundances of Bacteroidetes, Bacteroides, Proteobacteria Escherichia-Shigella and Pasteurella	1708:1804	Alterations in the abundances of Bacteroidetes, Bacteroides, Proteobacteria Escherichia-Shigella and Pasteurella may be involved in nutrient digestion and absorption, as well as the potential mechanisms connecting to the growth and development of IUGR in mammals.
31077497	0	35	theme	Gut	0:2	arg1	microbiota					4:13	Gut microbiota	0:13	Gut microbiota of newborn piglets with intrauterine growth restriction	0:69	Gut microbiota of newborn piglets with intrauterine growth restriction have lower diversity and different taxonomic abundances.
31077497	5	36	theme	IUGR	1083:1086	arg1	piglets					1088:1094	IUGR piglets	1083:1094	IUGR piglets	1083:1094	Correlation analysis showed that Bacteroides, Oscillibacter and Ruminococcaceae_UCG-002 compositions were positively associated with the body weight (BW) of IUGR piglets, nevertheless Proteobacteria and Escherichia-Shigella relative abundances were negatively correlated with the BW of IUGR piglets.
31077497	7	37	with	piglets	1628:1634	arg1	IUGR					1602:1605	IUGR	1602:1605	IUGR	1602:1605	CONCLUSIONS The present study profiled the intestinal microbiota of newborn piglets with IUGR and the newborn IUGR piglets have lower diversity and different taxonomic abundances.
31077497	3	38	theme	intestinal	365:374	arg1	microbiota					376:385	small intestinal microbiota	359:385	small intestinal microbiota	359:385	METHODS AND RESULTS We identified the alterations of small intestinal microbiota in IUGR piglets on 7, 21 and 28 days of age using 16S rRNA sequencing.
31077497	3	39	theme	16S	437:439	arg1	sequencing					446:455	16S rRNA sequencing	437:455	16S rRNA sequencing	437:455	METHODS AND RESULTS We identified the alterations of small intestinal microbiota in IUGR piglets on 7, 21 and 28 days of age using 16S rRNA sequencing.
31077497	4	40	contain	had	495:497	arg2	diversity					517:525	a decreased alpha diversity	499:525	a decreased alpha diversity of jejunum microbiota	499:547	The results showed that IUGR piglets had a decreased alpha diversity of jejunum microbiota at 7 and 21 days of age; had lower abundances of Bacteroidetes and Bacteroides in the jejunum at 7, 21 and 28 days of age, Oscillibacter in the jejunum at 21 days of age, and Firmicutes in the ileum at 21 days of age; whereas they had higher abundances of Proteobacteria and Pasteurella in the ileum at 21 days of age and Escherichia-Shigella in the jejunum at 28 days of age.
31077497	4	40	contain	had	495:497	arg1	piglets					487:493	IUGR piglets	482:493	IUGR piglets	482:493	The results showed that IUGR piglets had a decreased alpha diversity of jejunum microbiota at 7 and 21 days of age; had lower abundances of Bacteroidetes and Bacteroides in the jejunum at 7, 21 and 28 days of age, Oscillibacter in the jejunum at 21 days of age, and Firmicutes in the ileum at 21 days of age; whereas they had higher abundances of Proteobacteria and Pasteurella in the ileum at 21 days of age and Escherichia-Shigella in the jejunum at 28 days of age.
31077497	7	41	theme	lower	1641:1645	arg1	diversity					1647:1655	lower diversity	1641:1655	lower diversity	1641:1655	CONCLUSIONS The present study profiled the intestinal microbiota of newborn piglets with IUGR and the newborn IUGR piglets have lower diversity and different taxonomic abundances.
31077497	4	42	theme	jejunum	530:536	arg1	microbiota					538:547	jejunum microbiota	530:547	jejunum microbiota	530:547	The results showed that IUGR piglets had a decreased alpha diversity of jejunum microbiota at 7 and 21 days of age; had lower abundances of Bacteroidetes and Bacteroides in the jejunum at 7, 21 and 28 days of age, Oscillibacter in the jejunum at 21 days of age, and Firmicutes in the ileum at 21 days of age; whereas they had higher abundances of Proteobacteria and Pasteurella in the ileum at 21 days of age and Escherichia-Shigella in the jejunum at 28 days of age.
31077497	2	43	from	microbiota	278:287	arg1	piglets					297:303	IUGR piglets	292:303	IUGR piglets	292:303	The present study was conducted to unveil the alterations in intestinal microbiota in IUGR piglets.
31077497	3	44	theme	age	427:429	arg1	7					406:406	7	406:406	7	406:406	METHODS AND RESULTS We identified the alterations of small intestinal microbiota in IUGR piglets on 7, 21 and 28 days of age using 16S rRNA sequencing.
31077497	3	44	theme	age	427:429	arg1	age					427:429	age	427:429	age	427:429	METHODS AND RESULTS We identified the alterations of small intestinal microbiota in IUGR piglets on 7, 21 and 28 days of age using 16S rRNA sequencing.
31077497	0	45	theme	piglets	26:32	arg1	microbiota					4:13	Gut microbiota	0:13	Gut microbiota of newborn piglets with intrauterine growth restriction	0:69	Gut microbiota of newborn piglets with intrauterine growth restriction have lower diversity and different taxonomic abundances.
31077497	4	46	from	28 days	910:916	arg1	ileum					843:847	the ileum	839:847	the ileum at 21 days of age and Escherichia-Shigella in the jejunum at 28 days of age	839:923	The results showed that IUGR piglets had a decreased alpha diversity of jejunum microbiota at 7 and 21 days of age; had lower abundances of Bacteroidetes and Bacteroides in the jejunum at 7, 21 and 28 days of age, Oscillibacter in the jejunum at 21 days of age, and Firmicutes in the ileum at 21 days of age; whereas they had higher abundances of Proteobacteria and Pasteurella in the ileum at 21 days of age and Escherichia-Shigella in the jejunum at 28 days of age.
31077497	4	46	from	28 days	910:916	arg1	jejunum					899:905	the jejunum	895:905	the jejunum at 28 days of age	895:923	The results showed that IUGR piglets had a decreased alpha diversity of jejunum microbiota at 7 and 21 days of age; had lower abundances of Bacteroidetes and Bacteroides in the jejunum at 7, 21 and 28 days of age, Oscillibacter in the jejunum at 21 days of age, and Firmicutes in the ileum at 21 days of age; whereas they had higher abundances of Proteobacteria and Pasteurella in the ileum at 21 days of age and Escherichia-Shigella in the jejunum at 28 days of age.
31077497	6	47	theme	acid	1382:1385	arg1	metabolism					1387:1396	amino acid metabolism	1376:1396	amino acid metabolism	1376:1396	Gene function prediction analysis indicated that microbiota-associated carbohydrate metabolism, lipid metabolism, glycan biosynthesis and metabolism, amino acid metabolism, and xenobiotics biodegradation and metabolism were downregulated in the IUGR piglets compared to control piglets.
31077497	8	48	from	growth	1914:1919	arg1	mammals					1948:1954	mammals	1948:1954	mammals	1948:1954	Alterations in the abundances of Bacteroidetes, Bacteroides, Proteobacteria Escherichia-Shigella and Pasteurella may be involved in nutrient digestion and absorption, as well as the potential mechanisms connecting to the growth and development of IUGR in mammals.
31077497	0	49	theme	intrauterine	39:50	arg1	restriction					59:69	intrauterine growth restriction	39:69	intrauterine growth restriction	39:69	Gut microbiota of newborn piglets with intrauterine growth restriction have lower diversity and different taxonomic abundances.
31077497	5	50	theme	Correlation	926:936	arg1	analysis					938:945	Correlation analysis	926:945	Correlation analysis	926:945	Correlation analysis showed that Bacteroides, Oscillibacter and Ruminococcaceae_UCG-002 compositions were positively associated with the body weight (BW) of IUGR piglets, nevertheless Proteobacteria and Escherichia-Shigella relative abundances were negatively correlated with the BW of IUGR piglets.
31077497	4	51	theme	decreased	501:509	arg1	diversity					517:525	a decreased alpha diversity	499:525	a decreased alpha diversity of jejunum microbiota	499:547	The results showed that IUGR piglets had a decreased alpha diversity of jejunum microbiota at 7 and 21 days of age; had lower abundances of Bacteroidetes and Bacteroides in the jejunum at 7, 21 and 28 days of age, Oscillibacter in the jejunum at 21 days of age, and Firmicutes in the ileum at 21 days of age; whereas they had higher abundances of Proteobacteria and Pasteurella in the ileum at 21 days of age and Escherichia-Shigella in the jejunum at 28 days of age.
31077497	4	52	theme	age	715:717	arg1	21 days					704:710	21 days	704:710	21 days of age	704:717	The results showed that IUGR piglets had a decreased alpha diversity of jejunum microbiota at 7 and 21 days of age; had lower abundances of Bacteroidetes and Bacteroides in the jejunum at 7, 21 and 28 days of age, Oscillibacter in the jejunum at 21 days of age, and Firmicutes in the ileum at 21 days of age; whereas they had higher abundances of Proteobacteria and Pasteurella in the ileum at 21 days of age and Escherichia-Shigella in the jejunum at 28 days of age.
31077497	6	53	theme	IUGR	1471:1474	arg1	piglets					1476:1482	the IUGR piglets	1467:1482	the IUGR piglets	1467:1482	Gene function prediction analysis indicated that microbiota-associated carbohydrate metabolism, lipid metabolism, glycan biosynthesis and metabolism, amino acid metabolism, and xenobiotics biodegradation and metabolism were downregulated in the IUGR piglets compared to control piglets.
31077497	6	54	dep	xenobiotics	1403:1413	arg1	xenobiotics					1403:1413	xenobiotics biodegradation and metabolism	1403:1443	xenobiotics biodegradation and metabolism	1403:1443	Gene function prediction analysis indicated that microbiota-associated carbohydrate metabolism, lipid metabolism, glycan biosynthesis and metabolism, amino acid metabolism, and xenobiotics biodegradation and metabolism were downregulated in the IUGR piglets compared to control piglets.
31077497	6	54	dep	xenobiotics	1403:1413	arg1	metabolism					1434:1443	metabolism	1434:1443	metabolism	1434:1443	Gene function prediction analysis indicated that microbiota-associated carbohydrate metabolism, lipid metabolism, glycan biosynthesis and metabolism, amino acid metabolism, and xenobiotics biodegradation and metabolism were downregulated in the IUGR piglets compared to control piglets.
31077497	6	54	dep	xenobiotics	1403:1413	arg1	biodegradation					1415:1428	biodegradation	1415:1428	biodegradation	1415:1428	Gene function prediction analysis indicated that microbiota-associated carbohydrate metabolism, lipid metabolism, glycan biosynthesis and metabolism, amino acid metabolism, and xenobiotics biodegradation and metabolism were downregulated in the IUGR piglets compared to control piglets.
31077497	8	55	from	IUGR	1940:1943	arg1	mammals					1948:1954	mammals	1948:1954	mammals	1948:1954	Alterations in the abundances of Bacteroidetes, Bacteroides, Proteobacteria Escherichia-Shigella and Pasteurella may be involved in nutrient digestion and absorption, as well as the potential mechanisms connecting to the growth and development of IUGR in mammals.
31077497	0	56	theme	growth	52:57	arg1	restriction					59:69	intrauterine growth restriction	39:69	intrauterine growth restriction	39:69	Gut microbiota of newborn piglets with intrauterine growth restriction have lower diversity and different taxonomic abundances.
31077497	8	57	theme	potential	1875:1883	arg1	mechanisms					1885:1894	the potential mechanisms	1871:1894	the potential mechanisms connecting to the growth and development of IUGR in mammals	1871:1954	Alterations in the abundances of Bacteroidetes, Bacteroides, Proteobacteria Escherichia-Shigella and Pasteurella may be involved in nutrient digestion and absorption, as well as the potential mechanisms connecting to the growth and development of IUGR in mammals.
31077497	8	58	from	development	1925:1935	arg1	mammals					1948:1954	mammals	1948:1954	mammals	1948:1954	Alterations in the abundances of Bacteroidetes, Bacteroides, Proteobacteria Escherichia-Shigella and Pasteurella may be involved in nutrient digestion and absorption, as well as the potential mechanisms connecting to the growth and development of IUGR in mammals.
31077497	5	59	theme	relative	1150:1157	arg1	abundances					1159:1168	Escherichia-Shigella relative abundances	1129:1168	Escherichia-Shigella relative abundances	1129:1168	Correlation analysis showed that Bacteroides, Oscillibacter and Ruminococcaceae_UCG-002 compositions were positively associated with the body weight (BW) of IUGR piglets, nevertheless Proteobacteria and Escherichia-Shigella relative abundances were negatively correlated with the BW of IUGR piglets.
31077497	9	60	theme	IUGR piglets	2124:2135	arg1	growth					2098:2103	growth	2098:2103	growth	2098:2103	SIGNIFICANCE AND IMPACT OF THE STUDY The small intestinal microbiota were highly shaped in the IUGR piglets, which might further mediate the growth and development of IUGR piglets; and the gut microbiota could serve as a potential target for IUGR treatment.
31077497	9	60	theme	IUGR piglets	2124:2135	arg1	development					2109:2119	development	2109:2119	development	2109:2119	SIGNIFICANCE AND IMPACT OF THE STUDY The small intestinal microbiota were highly shaped in the IUGR piglets, which might further mediate the growth and development of IUGR piglets; and the gut microbiota could serve as a potential target for IUGR treatment.
31077497	6	61	theme	Gene	1226:1229	arg1	analysis					1251:1258	Gene function prediction analysis	1226:1258	Gene function prediction analysis	1226:1258	Gene function prediction analysis indicated that microbiota-associated carbohydrate metabolism, lipid metabolism, glycan biosynthesis and metabolism, amino acid metabolism, and xenobiotics biodegradation and metabolism were downregulated in the IUGR piglets compared to control piglets.
31077497	0	62	contain	have	71:74	arg2	abundances					116:125	different taxonomic abundances	96:125	different taxonomic abundances	96:125	Gut microbiota of newborn piglets with intrauterine growth restriction have lower diversity and different taxonomic abundances.
31077497	0	62	contain	have	71:74	arg2	diversity					82:90	lower diversity	76:90	lower diversity	76:90	Gut microbiota of newborn piglets with intrauterine growth restriction have lower diversity and different taxonomic abundances.
31077497	0	62	contain	have	71:74	arg1	microbiota					4:13	Gut microbiota	0:13	Gut microbiota of newborn piglets with intrauterine growth restriction	0:69	Gut microbiota of newborn piglets with intrauterine growth restriction have lower diversity and different taxonomic abundances.
31077497	4	63	from	7	646:646	arg1	jejunum					635:641	the jejunum	631:641	the jejunum at 7	631:646	The results showed that IUGR piglets had a decreased alpha diversity of jejunum microbiota at 7 and 21 days of age; had lower abundances of Bacteroidetes and Bacteroides in the jejunum at 7, 21 and 28 days of age, Oscillibacter in the jejunum at 21 days of age, and Firmicutes in the ileum at 21 days of age; whereas they had higher abundances of Proteobacteria and Pasteurella in the ileum at 21 days of age and Escherichia-Shigella in the jejunum at 28 days of age.
31077497	4	63	from	7	646:646	arg1	28 days					656:662	28 days	656:662	28 days	656:662	The results showed that IUGR piglets had a decreased alpha diversity of jejunum microbiota at 7 and 21 days of age; had lower abundances of Bacteroidetes and Bacteroides in the jejunum at 7, 21 and 28 days of age, Oscillibacter in the jejunum at 21 days of age, and Firmicutes in the ileum at 21 days of age; whereas they had higher abundances of Proteobacteria and Pasteurella in the ileum at 21 days of age and Escherichia-Shigella in the jejunum at 28 days of age.
31077497	4	64	from	jejunum	635:641	arg1	abundances					584:593	lower abundances	578:593	lower abundances of Bacteroidetes and Bacteroides in the jejunum at 7, 21 and 28 days of age, Oscillibacter in the jejunum at 21 days of age, and Firmicutes in the ileum	578:746	The results showed that IUGR piglets had a decreased alpha diversity of jejunum microbiota at 7 and 21 days of age; had lower abundances of Bacteroidetes and Bacteroides in the jejunum at 7, 21 and 28 days of age, Oscillibacter in the jejunum at 21 days of age, and Firmicutes in the ileum at 21 days of age; whereas they had higher abundances of Proteobacteria and Pasteurella in the ileum at 21 days of age and Escherichia-Shigella in the jejunum at 28 days of age.
31077497	5	65	theme	Escherichia-Shigella	1129:1148	arg1	abundances					1159:1168	Escherichia-Shigella relative abundances	1129:1168	Escherichia-Shigella relative abundances	1129:1168	Correlation analysis showed that Bacteroides, Oscillibacter and Ruminococcaceae_UCG-002 compositions were positively associated with the body weight (BW) of IUGR piglets, nevertheless Proteobacteria and Escherichia-Shigella relative abundances were negatively correlated with the BW of IUGR piglets.
31077497	8	66	from	mammals	1948:1954	arg1	development					1925:1935	development	1925:1935	development	1925:1935	Alterations in the abundances of Bacteroidetes, Bacteroides, Proteobacteria Escherichia-Shigella and Pasteurella may be involved in nutrient digestion and absorption, as well as the potential mechanisms connecting to the growth and development of IUGR in mammals.
31077497	8	66	from	mammals	1948:1954	arg1	growth					1914:1919	growth	1914:1919	growth	1914:1919	Alterations in the abundances of Bacteroidetes, Bacteroides, Proteobacteria Escherichia-Shigella and Pasteurella may be involved in nutrient digestion and absorption, as well as the potential mechanisms connecting to the growth and development of IUGR in mammals.
31077497	9	67	theme	gut	2146:2148	arg1	microbiota					2150:2159	the gut microbiota	2142:2159	the gut microbiota	2142:2159	SIGNIFICANCE AND IMPACT OF THE STUDY The small intestinal microbiota were highly shaped in the IUGR piglets, which might further mediate the growth and development of IUGR piglets; and the gut microbiota could serve as a potential target for IUGR treatment.
31077497	9	67	theme	gut	2146:2148	arg1	target					2188:2193	a potential target	2176:2193	a potential target for IUGR treatment	2176:2212	SIGNIFICANCE AND IMPACT OF THE STUDY The small intestinal microbiota were highly shaped in the IUGR piglets, which might further mediate the growth and development of IUGR piglets; and the gut microbiota could serve as a potential target for IUGR treatment.
31077497	2	68	theme	intestinal	267:276	arg1	microbiota					278:287	intestinal microbiota	267:287	intestinal microbiota in IUGR piglets	267:303	The present study was conducted to unveil the alterations in intestinal microbiota in IUGR piglets.
31077497	6	69	theme	glycan	1340:1345	arg1	biosynthesis					1347:1358	glycan biosynthesis	1340:1358	glycan biosynthesis	1340:1358	Gene function prediction analysis indicated that microbiota-associated carbohydrate metabolism, lipid metabolism, glycan biosynthesis and metabolism, amino acid metabolism, and xenobiotics biodegradation and metabolism were downregulated in the IUGR piglets compared to control piglets.
31077497	1	70	theme	prevalent	176:184	arg1	problem					186:192	a prevalent problem	174:192	a prevalent problem in mammals	174:203	AIM Intrauterine growth retardation (IUGR) is a prevalent problem in mammals.
31077497	1	70	theme	prevalent	176:184	arg1	retardation					152:162	AIM Intrauterine growth retardation	128:162	AIM Intrauterine growth retardation (IUGR)	128:169	AIM Intrauterine growth retardation (IUGR) is a prevalent problem in mammals.
31077497	7	71	theme	piglets	1628:1634	arg1	microbiota					1567:1576	the intestinal microbiota	1552:1576	the intestinal microbiota of newborn piglets with IUGR and the newborn IUGR piglets	1552:1634	CONCLUSIONS The present study profiled the intestinal microbiota of newborn piglets with IUGR and the newborn IUGR piglets have lower diversity and different taxonomic abundances.
31077497	6	72	theme	control	1496:1502	arg1	piglets					1504:1510	control piglets	1496:1510	control piglets	1496:1510	Gene function prediction analysis indicated that microbiota-associated carbohydrate metabolism, lipid metabolism, glycan biosynthesis and metabolism, amino acid metabolism, and xenobiotics biodegradation and metabolism were downregulated in the IUGR piglets compared to control piglets.
31077497	7	73	theme	newborn	1615:1621	arg1	piglets					1628:1634	the newborn IUGR piglets	1611:1634	the newborn IUGR piglets	1611:1634	CONCLUSIONS The present study profiled the intestinal microbiota of newborn piglets with IUGR and the newborn IUGR piglets have lower diversity and different taxonomic abundances.
31077497	4	74	theme	lower	578:582	arg1	abundances					584:593	lower abundances	578:593	lower abundances of Bacteroidetes and Bacteroides in the jejunum at 7, 21 and 28 days of age, Oscillibacter in the jejunum at 21 days of age, and Firmicutes in the ileum	578:746	The results showed that IUGR piglets had a decreased alpha diversity of jejunum microbiota at 7 and 21 days of age; had lower abundances of Bacteroidetes and Bacteroides in the jejunum at 7, 21 and 28 days of age, Oscillibacter in the jejunum at 21 days of age, and Firmicutes in the ileum at 21 days of age; whereas they had higher abundances of Proteobacteria and Pasteurella in the ileum at 21 days of age and Escherichia-Shigella in the jejunum at 28 days of age.
31077497	9	75	theme	potential	2178:2186	arg1	microbiota					2150:2159	the gut microbiota	2142:2159	the gut microbiota	2142:2159	SIGNIFICANCE AND IMPACT OF THE STUDY The small intestinal microbiota were highly shaped in the IUGR piglets, which might further mediate the growth and development of IUGR piglets; and the gut microbiota could serve as a potential target for IUGR treatment.
31077497	9	75	theme	potential	2178:2186	arg1	target					2188:2193	a potential target	2176:2193	a potential target for IUGR treatment	2176:2212	SIGNIFICANCE AND IMPACT OF THE STUDY The small intestinal microbiota were highly shaped in the IUGR piglets, which might further mediate the growth and development of IUGR piglets; and the gut microbiota could serve as a potential target for IUGR treatment.
31077497	6	76	theme	microbiota-associated	1275:1295	arg1	metabolism					1310:1319	microbiota-associated carbohydrate metabolism	1275:1319	microbiota-associated carbohydrate metabolism	1275:1319	Gene function prediction analysis indicated that microbiota-associated carbohydrate metabolism, lipid metabolism, glycan biosynthesis and metabolism, amino acid metabolism, and xenobiotics biodegradation and metabolism were downregulated in the IUGR piglets compared to control piglets.
31077497	3	77	from	alterations	344:354	arg1	piglets					395:401	IUGR piglets	390:401	IUGR piglets	390:401	METHODS AND RESULTS We identified the alterations of small intestinal microbiota in IUGR piglets on 7, 21 and 28 days of age using 16S rRNA sequencing.
31077497	8	78	theme	Bacteroides	1741:1751	arg1	abundances					1712:1721	the abundances	1708:1721	the abundances of Bacteroidetes, Bacteroides, Proteobacteria Escherichia-Shigella and Pasteurella	1708:1804	Alterations in the abundances of Bacteroidetes, Bacteroides, Proteobacteria Escherichia-Shigella and Pasteurella may be involved in nutrient digestion and absorption, as well as the potential mechanisms connecting to the growth and development of IUGR in mammals.
31077497	5	79	theme	Ruminococcaceae_UCG-002	990:1012	arg1	compositions					1014:1025	Ruminococcaceae_UCG-002 compositions	990:1025	Ruminococcaceae_UCG-002 compositions	990:1025	Correlation analysis showed that Bacteroides, Oscillibacter and Ruminococcaceae_UCG-002 compositions were positively associated with the body weight (BW) of IUGR piglets, nevertheless Proteobacteria and Escherichia-Shigella relative abundances were negatively correlated with the BW of IUGR piglets.
31077497	8	80	theme	Proteobacteria	1754:1767	arg1	Escherichia-Shigella					1769:1788	Proteobacteria Escherichia-Shigella	1754:1788	Proteobacteria Escherichia-Shigella	1754:1788	Alterations in the abundances of Bacteroidetes, Bacteroides, Proteobacteria Escherichia-Shigella and Pasteurella may be involved in nutrient digestion and absorption, as well as the potential mechanisms connecting to the growth and development of IUGR in mammals.
31077497	4	81	theme	higher	784:789	arg1	abundances					791:800	higher abundances	784:800	higher abundances	784:800	The results showed that IUGR piglets had a decreased alpha diversity of jejunum microbiota at 7 and 21 days of age; had lower abundances of Bacteroidetes and Bacteroides in the jejunum at 7, 21 and 28 days of age, Oscillibacter in the jejunum at 21 days of age, and Firmicutes in the ileum at 21 days of age; whereas they had higher abundances of Proteobacteria and Pasteurella in the ileum at 21 days of age and Escherichia-Shigella in the jejunum at 28 days of age.
31077497	5	82	theme	IUGR	1212:1215	arg1	piglets					1217:1223	IUGR piglets	1212:1223	IUGR piglets	1212:1223	Correlation analysis showed that Bacteroides, Oscillibacter and Ruminococcaceae_UCG-002 compositions were positively associated with the body weight (BW) of IUGR piglets, nevertheless Proteobacteria and Escherichia-Shigella relative abundances were negatively correlated with the BW of IUGR piglets.
31077497	6	83	theme	prediction	1240:1249	arg1	analysis					1251:1258	Gene function prediction analysis	1226:1258	Gene function prediction analysis	1226:1258	Gene function prediction analysis indicated that microbiota-associated carbohydrate metabolism, lipid metabolism, glycan biosynthesis and metabolism, amino acid metabolism, and xenobiotics biodegradation and metabolism were downregulated in the IUGR piglets compared to control piglets.
31077497	1	84	theme	Intrauterine	132:143	arg1	problem					186:192	a prevalent problem	174:192	a prevalent problem in mammals	174:203	AIM Intrauterine growth retardation (IUGR) is a prevalent problem in mammals.
31077497	1	84	theme	Intrauterine	132:143	arg1	IUGR					165:168	IUGR	165:168	IUGR	165:168	AIM Intrauterine growth retardation (IUGR) is a prevalent problem in mammals.
31077497	1	84	theme	Intrauterine	132:143	arg1	retardation					152:162	AIM Intrauterine growth retardation	128:162	AIM Intrauterine growth retardation (IUGR)	128:169	AIM Intrauterine growth retardation (IUGR) is a prevalent problem in mammals.
31077497	7	85	theme	newborn	1581:1587	arg1	piglets					1589:1595	newborn piglets	1581:1595	newborn piglets with IUGR	1581:1605	CONCLUSIONS The present study profiled the intestinal microbiota of newborn piglets with IUGR and the newborn IUGR piglets have lower diversity and different taxonomic abundances.
31077497	8	86	theme	Bacteroidetes	1726:1738	arg1	abundances					1712:1721	the abundances	1708:1721	the abundances of Bacteroidetes, Bacteroides, Proteobacteria Escherichia-Shigella and Pasteurella	1708:1804	Alterations in the abundances of Bacteroidetes, Bacteroides, Proteobacteria Escherichia-Shigella and Pasteurella may be involved in nutrient digestion and absorption, as well as the potential mechanisms connecting to the growth and development of IUGR in mammals.
31077497	1	87	from	problem	186:192	arg1	mammals					197:203	mammals	197:203	mammals	197:203	AIM Intrauterine growth retardation (IUGR) is a prevalent problem in mammals.
31077497	4	88	from	28 days	656:662	arg1	7					646:646	7	646:646	7	646:646	The results showed that IUGR piglets had a decreased alpha diversity of jejunum microbiota at 7 and 21 days of age; had lower abundances of Bacteroidetes and Bacteroides in the jejunum at 7, 21 and 28 days of age, Oscillibacter in the jejunum at 21 days of age, and Firmicutes in the ileum at 21 days of age; whereas they had higher abundances of Proteobacteria and Pasteurella in the ileum at 21 days of age and Escherichia-Shigella in the jejunum at 28 days of age.
31077497	4	88	from	28 days	656:662	arg1	jejunum					693:699	the jejunum	689:699	the jejunum at 21 days of age	689:717	The results showed that IUGR piglets had a decreased alpha diversity of jejunum microbiota at 7 and 21 days of age; had lower abundances of Bacteroidetes and Bacteroides in the jejunum at 7, 21 and 28 days of age, Oscillibacter in the jejunum at 21 days of age, and Firmicutes in the ileum at 21 days of age; whereas they had higher abundances of Proteobacteria and Pasteurella in the ileum at 21 days of age and Escherichia-Shigella in the jejunum at 28 days of age.
31077497	4	88	from	28 days	656:662	arg1	jejunum					635:641	the jejunum	631:641	the jejunum at 7	631:646	The results showed that IUGR piglets had a decreased alpha diversity of jejunum microbiota at 7 and 21 days of age; had lower abundances of Bacteroidetes and Bacteroides in the jejunum at 7, 21 and 28 days of age, Oscillibacter in the jejunum at 21 days of age, and Firmicutes in the ileum at 21 days of age; whereas they had higher abundances of Proteobacteria and Pasteurella in the ileum at 21 days of age and Escherichia-Shigella in the jejunum at 28 days of age.
31077497	4	88	from	28 days	656:662	arg1	21 days					704:710	21 days	704:710	21 days of age	704:717	The results showed that IUGR piglets had a decreased alpha diversity of jejunum microbiota at 7 and 21 days of age; had lower abundances of Bacteroidetes and Bacteroides in the jejunum at 7, 21 and 28 days of age, Oscillibacter in the jejunum at 21 days of age, and Firmicutes in the ileum at 21 days of age; whereas they had higher abundances of Proteobacteria and Pasteurella in the ileum at 21 days of age and Escherichia-Shigella in the jejunum at 28 days of age.
31077497	4	88	from	28 days	656:662	arg1	ileum					742:746	the ileum	738:746	the ileum	738:746	The results showed that IUGR piglets had a decreased alpha diversity of jejunum microbiota at 7 and 21 days of age; had lower abundances of Bacteroidetes and Bacteroides in the jejunum at 7, 21 and 28 days of age, Oscillibacter in the jejunum at 21 days of age, and Firmicutes in the ileum at 21 days of age; whereas they had higher abundances of Proteobacteria and Pasteurella in the ileum at 21 days of age and Escherichia-Shigella in the jejunum at 28 days of age.
31077497	4	89	theme	age	863:865	arg1	21 days					852:858	21 days	852:858	21 days of age and Escherichia-Shigella	852:890	The results showed that IUGR piglets had a decreased alpha diversity of jejunum microbiota at 7 and 21 days of age; had lower abundances of Bacteroidetes and Bacteroides in the jejunum at 7, 21 and 28 days of age, Oscillibacter in the jejunum at 21 days of age, and Firmicutes in the ileum at 21 days of age; whereas they had higher abundances of Proteobacteria and Pasteurella in the ileum at 21 days of age and Escherichia-Shigella in the jejunum at 28 days of age.
31077497	9	90	theme	intestinal	2004:2013	arg1	microbiota					2015:2024	The small intestinal microbiota	1994:2024	The small intestinal microbiota	1994:2024	SIGNIFICANCE AND IMPACT OF THE STUDY The small intestinal microbiota were highly shaped in the IUGR piglets, which might further mediate the growth and development of IUGR piglets; and the gut microbiota could serve as a potential target for IUGR treatment.
31077497	7	91	with	piglets	1589:1595	arg1	IUGR					1602:1605	IUGR	1602:1605	IUGR	1602:1605	CONCLUSIONS The present study profiled the intestinal microbiota of newborn piglets with IUGR and the newborn IUGR piglets have lower diversity and different taxonomic abundances.
31077497	4	92	contain	had	780:782	arg1	they					775:778	they	775:778	they	775:778	The results showed that IUGR piglets had a decreased alpha diversity of jejunum microbiota at 7 and 21 days of age; had lower abundances of Bacteroidetes and Bacteroides in the jejunum at 7, 21 and 28 days of age, Oscillibacter in the jejunum at 21 days of age, and Firmicutes in the ileum at 21 days of age; whereas they had higher abundances of Proteobacteria and Pasteurella in the ileum at 21 days of age and Escherichia-Shigella in the jejunum at 28 days of age.
31077497	4	92	contain	had	780:782	arg2	abundances					791:800	higher abundances	784:800	higher abundances	784:800	The results showed that IUGR piglets had a decreased alpha diversity of jejunum microbiota at 7 and 21 days of age; had lower abundances of Bacteroidetes and Bacteroides in the jejunum at 7, 21 and 28 days of age, Oscillibacter in the jejunum at 21 days of age, and Firmicutes in the ileum at 21 days of age; whereas they had higher abundances of Proteobacteria and Pasteurella in the ileum at 21 days of age and Escherichia-Shigella in the jejunum at 28 days of age.
31077497	4	93	theme	Escherichia-Shigella	871:890	arg1	21 days					852:858	21 days	852:858	21 days of age and Escherichia-Shigella	852:890	The results showed that IUGR piglets had a decreased alpha diversity of jejunum microbiota at 7 and 21 days of age; had lower abundances of Bacteroidetes and Bacteroides in the jejunum at 7, 21 and 28 days of age, Oscillibacter in the jejunum at 21 days of age, and Firmicutes in the ileum at 21 days of age; whereas they had higher abundances of Proteobacteria and Pasteurella in the ileum at 21 days of age and Escherichia-Shigella in the jejunum at 28 days of age.
31077497	0	94	theme	different	96:104	arg1	abundances					116:125	different taxonomic abundances	96:125	different taxonomic abundances	96:125	Gut microbiota of newborn piglets with intrauterine growth restriction have lower diversity and different taxonomic abundances.
31077497	8	95	theme	nutrient	1825:1832	arg1	digestion					1834:1842	nutrient digestion	1825:1842	nutrient digestion	1825:1842	Alterations in the abundances of Bacteroidetes, Bacteroides, Proteobacteria Escherichia-Shigella and Pasteurella may be involved in nutrient digestion and absorption, as well as the potential mechanisms connecting to the growth and development of IUGR in mammals.
31077497	3	96	theme	small	359:363	arg1	microbiota					376:385	small intestinal microbiota	359:385	small intestinal microbiota	359:385	METHODS AND RESULTS We identified the alterations of small intestinal microbiota in IUGR piglets on 7, 21 and 28 days of age using 16S rRNA sequencing.
31077497	6	97	theme	lipid	1322:1326	arg1	metabolism					1328:1337	lipid metabolism	1322:1337	lipid metabolism	1322:1337	Gene function prediction analysis indicated that microbiota-associated carbohydrate metabolism, lipid metabolism, glycan biosynthesis and metabolism, amino acid metabolism, and xenobiotics biodegradation and metabolism were downregulated in the IUGR piglets compared to control piglets.
31077497	3	98	theme	IUGR	390:393	arg1	piglets					395:401	IUGR piglets	390:401	IUGR piglets	390:401	METHODS AND RESULTS We identified the alterations of small intestinal microbiota in IUGR piglets on 7, 21 and 28 days of age using 16S rRNA sequencing.
31077497	4	99	theme	age	667:669	arg1	age					667:669	age	667:669	age	667:669	The results showed that IUGR piglets had a decreased alpha diversity of jejunum microbiota at 7 and 21 days of age; had lower abundances of Bacteroidetes and Bacteroides in the jejunum at 7, 21 and 28 days of age, Oscillibacter in the jejunum at 21 days of age, and Firmicutes in the ileum at 21 days of age; whereas they had higher abundances of Proteobacteria and Pasteurella in the ileum at 21 days of age and Escherichia-Shigella in the jejunum at 28 days of age.
31077497	4	99	theme	age	667:669	arg1	21					649:650	21	649:650	21	649:650	The results showed that IUGR piglets had a decreased alpha diversity of jejunum microbiota at 7 and 21 days of age; had lower abundances of Bacteroidetes and Bacteroides in the jejunum at 7, 21 and 28 days of age, Oscillibacter in the jejunum at 21 days of age, and Firmicutes in the ileum at 21 days of age; whereas they had higher abundances of Proteobacteria and Pasteurella in the ileum at 21 days of age and Escherichia-Shigella in the jejunum at 28 days of age.
31077497	0	100	theme	newborn	18:24	arg1	piglets					26:32	newborn piglets	18:32	newborn piglets	18:32	Gut microbiota of newborn piglets with intrauterine growth restriction have lower diversity and different taxonomic abundances.
31077497	5	101	theme	piglets	1088:1094	arg1	BW					1076:1077	BW	1076:1077	BW	1076:1077	Correlation analysis showed that Bacteroides, Oscillibacter and Ruminococcaceae_UCG-002 compositions were positively associated with the body weight (BW) of IUGR piglets, nevertheless Proteobacteria and Escherichia-Shigella relative abundances were negatively correlated with the BW of IUGR piglets.
31077497	5	101	theme	piglets	1088:1094	arg1	weight					1068:1073	the body weight	1059:1073	the body weight (BW) of IUGR piglets	1059:1094	Correlation analysis showed that Bacteroides, Oscillibacter and Ruminococcaceae_UCG-002 compositions were positively associated with the body weight (BW) of IUGR piglets, nevertheless Proteobacteria and Escherichia-Shigella relative abundances were negatively correlated with the BW of IUGR piglets.
31077497	7	102	contain	have	1636:1639	arg1	CONCLUSIONS					1513:1523	CONCLUSIONS	1513:1523	CONCLUSIONS The present study profiled the intestinal microbiota of newborn piglets with IUGR and the newborn IUGR piglets	1513:1634	CONCLUSIONS The present study profiled the intestinal microbiota of newborn piglets with IUGR and the newborn IUGR piglets have lower diversity and different taxonomic abundances.
31077497	7	102	contain	have	1636:1639	arg2	abundances					1681:1690	different taxonomic abundances	1661:1690	different taxonomic abundances	1661:1690	CONCLUSIONS The present study profiled the intestinal microbiota of newborn piglets with IUGR and the newborn IUGR piglets have lower diversity and different taxonomic abundances.
31077497	7	102	contain	have	1636:1639	arg2	diversity					1647:1655	lower diversity	1641:1655	lower diversity	1641:1655	CONCLUSIONS The present study profiled the intestinal microbiota of newborn piglets with IUGR and the newborn IUGR piglets have lower diversity and different taxonomic abundances.
31077497	7	103	theme	different	1661:1669	arg1	abundances					1681:1690	different taxonomic abundances	1661:1690	different taxonomic abundances	1661:1690	CONCLUSIONS The present study profiled the intestinal microbiota of newborn piglets with IUGR and the newborn IUGR piglets have lower diversity and different taxonomic abundances.
31077497	3	104	theme	microbiota	376:385	arg1	alterations					344:354	the alterations	340:354	the alterations of small intestinal microbiota in IUGR piglets	340:401	METHODS AND RESULTS We identified the alterations of small intestinal microbiota in IUGR piglets on 7, 21 and 28 days of age using 16S rRNA sequencing.
31077497	4	105	theme	microbiota	538:547	arg1	diversity					517:525	a decreased alpha diversity	499:525	a decreased alpha diversity of jejunum microbiota	499:547	The results showed that IUGR piglets had a decreased alpha diversity of jejunum microbiota at 7 and 21 days of age; had lower abundances of Bacteroidetes and Bacteroides in the jejunum at 7, 21 and 28 days of age, Oscillibacter in the jejunum at 21 days of age, and Firmicutes in the ileum at 21 days of age; whereas they had higher abundances of Proteobacteria and Pasteurella in the ileum at 21 days of age and Escherichia-Shigella in the jejunum at 28 days of age.
31077497	3	106	theme	rRNA	441:444	arg1	sequencing					446:455	16S rRNA sequencing	437:455	16S rRNA sequencing	437:455	METHODS AND RESULTS We identified the alterations of small intestinal microbiota in IUGR piglets on 7, 21 and 28 days of age using 16S rRNA sequencing.
31077497	7	107	theme	present	1529:1535	arg1	study					1537:1541	The present study	1525:1541	The present study	1525:1541	CONCLUSIONS The present study profiled the intestinal microbiota of newborn piglets with IUGR and the newborn IUGR piglets have lower diversity and different taxonomic abundances.
31077497	6	108	theme	amino	1376:1380	arg1	metabolism					1387:1396	amino acid metabolism	1376:1396	amino acid metabolism	1376:1396	Gene function prediction analysis indicated that microbiota-associated carbohydrate metabolism, lipid metabolism, glycan biosynthesis and metabolism, amino acid metabolism, and xenobiotics biodegradation and metabolism were downregulated in the IUGR piglets compared to control piglets.
31077497	9	109	from	piglets	2057:2063	arg1	shaped					2038:2043	shaped	2038:2043	shaped	2038:2043	SIGNIFICANCE AND IMPACT OF THE STUDY The small intestinal microbiota were highly shaped in the IUGR piglets, which might further mediate the growth and development of IUGR piglets; and the gut microbiota could serve as a potential target for IUGR treatment.
31077497	4	110	theme	alpha	511:515	arg1	diversity					517:525	a decreased alpha diversity	499:525	a decreased alpha diversity of jejunum microbiota	499:547	The results showed that IUGR piglets had a decreased alpha diversity of jejunum microbiota at 7 and 21 days of age; had lower abundances of Bacteroidetes and Bacteroides in the jejunum at 7, 21 and 28 days of age, Oscillibacter in the jejunum at 21 days of age, and Firmicutes in the ileum at 21 days of age; whereas they had higher abundances of Proteobacteria and Pasteurella in the ileum at 21 days of age and Escherichia-Shigella in the jejunum at 28 days of age.
31077497	0	111	with	microbiota	4:13	arg1	restriction					59:69	intrauterine growth restriction	39:69	intrauterine growth restriction	39:69	Gut microbiota of newborn piglets with intrauterine growth restriction have lower diversity and different taxonomic abundances.
31077497	4	112	theme	age	569:571	arg1	age					569:571	age	569:571	age	569:571	The results showed that IUGR piglets had a decreased alpha diversity of jejunum microbiota at 7 and 21 days of age; had lower abundances of Bacteroidetes and Bacteroides in the jejunum at 7, 21 and 28 days of age, Oscillibacter in the jejunum at 21 days of age, and Firmicutes in the ileum at 21 days of age; whereas they had higher abundances of Proteobacteria and Pasteurella in the ileum at 21 days of age and Escherichia-Shigella in the jejunum at 28 days of age.
31077497	4	112	theme	age	569:571	arg1	7					552:552	7	552:552	7	552:552	The results showed that IUGR piglets had a decreased alpha diversity of jejunum microbiota at 7 and 21 days of age; had lower abundances of Bacteroidetes and Bacteroides in the jejunum at 7, 21 and 28 days of age, Oscillibacter in the jejunum at 21 days of age, and Firmicutes in the ileum at 21 days of age; whereas they had higher abundances of Proteobacteria and Pasteurella in the ileum at 21 days of age and Escherichia-Shigella in the jejunum at 28 days of age.
31077497	8	113	theme	Escherichia-Shigella	1769:1788	arg1	abundances					1712:1721	the abundances	1708:1721	the abundances of Bacteroidetes, Bacteroides, Proteobacteria Escherichia-Shigella and Pasteurella	1708:1804	Alterations in the abundances of Bacteroidetes, Bacteroides, Proteobacteria Escherichia-Shigella and Pasteurella may be involved in nutrient digestion and absorption, as well as the potential mechanisms connecting to the growth and development of IUGR in mammals.
31077497	7	114	dep	CONCLUSIONS	1513:1523	arg1	profiled					1543:1550	profiled	1543:1550	profiled the intestinal microbiota of newborn piglets with IUGR and the newborn IUGR piglets	1543:1634	CONCLUSIONS The present study profiled the intestinal microbiota of newborn piglets with IUGR and the newborn IUGR piglets have lower diversity and different taxonomic abundances.
31077497	9	115	from	shaped	2038:2043	arg1	piglets					2057:2063	the IUGR piglets	2048:2063	the IUGR piglets	2048:2063	SIGNIFICANCE AND IMPACT OF THE STUDY The small intestinal microbiota were highly shaped in the IUGR piglets, which might further mediate the growth and development of IUGR piglets; and the gut microbiota could serve as a potential target for IUGR treatment.
29476400	6	0	with	thermo-stable	860:872	arg1	activity					884:891	90% activity	880:891	90% activity retained after 1 h at 45 °C and 70% activity retained after 4 h	880:955	This enzyme is also significantly thermo-stable, with 90% activity retained after 1 h at 45 °C and 70% activity retained after 4 h, even at 50 °C. Biochemical characterization revealed that the enzyme has higher substrate specificity for 3-O-linked flavonoid β-rutinosides like rutin and kaempferol-3-O-rutinoside, than for 7-O-linked flavonoid β-rutinoside like hesperidin.
29476400	10	1	theme	kcat/K	1595:1600	arg1	efficiency					1583:1592	the catalytic efficiency	1569:1592	the catalytic efficiency (kcat/K m )	1569:1604	Overall, the catalytic efficiency (kcat/K m ) was highest for kaempferol-3-O-rutinoside.
29476400	10	1	theme	kcat/K	1595:1600	arg1	m					1602:1602	kcat/K m	1595:1602	kcat/K m	1595:1602	Overall, the catalytic efficiency (kcat/K m ) was highest for kaempferol-3-O-rutinoside.
29476400	6	2	theme	Biochemical	973:983	arg1	characterization					985:1000	Biochemical characterization	973:1000	Biochemical characterization	973:1000	This enzyme is also significantly thermo-stable, with 90% activity retained after 1 h at 45 °C and 70% activity retained after 4 h, even at 50 °C. Biochemical characterization revealed that the enzyme has higher substrate specificity for 3-O-linked flavonoid β-rutinosides like rutin and kaempferol-3-O-rutinoside, than for 7-O-linked flavonoid β-rutinoside like hesperidin.
29476400	5	3	theme	Optimal	750:756	arg1	activity					768:775	Optimal enzymatic activity	750:775	Optimal enzymatic activity	750:775	Optimal enzymatic activity was observed at pH 4.0 and temperature of 45 °C.
29476400	2	4	theme	niger	430:434	arg1	rutinosidase					436:447	Aspergillus niger rutinosidase	418:447	Aspergillus niger rutinosidase	418:447	We identified a hypothetical protein (annotated as AO090012000917) encoded in the Aspergillus oryzae genome that exhibits sequence similarity with Aspergillus niger rutinosidase.
29476400	6	5	dep	50 °C.	966:971	arg1	revealed					1002:1009	revealed	1002:1009	revealed that the enzyme has higher substrate specificity for 3-O-linked flavonoid β-rutinosides like rutin and kaempferol-3-O-rutinoside, than for 7-O-linked flavonoid β-rutinoside like hesperidin	1002:1198	This enzyme is also significantly thermo-stable, with 90% activity retained after 1 h at 45 °C and 70% activity retained after 4 h, even at 50 °C. Biochemical characterization revealed that the enzyme has higher substrate specificity for 3-O-linked flavonoid β-rutinosides like rutin and kaempferol-3-O-rutinoside, than for 7-O-linked flavonoid β-rutinoside like hesperidin.
29476400	5	6	theme	45 °C	819:823	arg1	pH					793:794	pH 4.0	793:798	pH 4.0	793:798	Optimal enzymatic activity was observed at pH 4.0 and temperature of 45 °C.
29476400	5	6	theme	45 °C	819:823	arg1	temperature					804:814	temperature	804:814	temperature of 45 °C	804:823	Optimal enzymatic activity was observed at pH 4.0 and temperature of 45 °C.
29476400	5	7	theme	enzymatic	758:766	arg1	activity					768:775	Optimal enzymatic activity	750:775	Optimal enzymatic activity	750:775	Optimal enzymatic activity was observed at pH 4.0 and temperature of 45 °C.
29476400	6	8	theme	flavonoid	1075:1083	arg1	β-rutinosides					1085:1097	3-O-linked flavonoid β-rutinosides	1064:1097	3-O-linked flavonoid β-rutinosides	1064:1097	This enzyme is also significantly thermo-stable, with 90% activity retained after 1 h at 45 °C and 70% activity retained after 4 h, even at 50 °C. Biochemical characterization revealed that the enzyme has higher substrate specificity for 3-O-linked flavonoid β-rutinosides like rutin and kaempferol-3-O-rutinoside, than for 7-O-linked flavonoid β-rutinoside like hesperidin.
29476400	10	9	theme	catalytic	1573:1581	arg1	efficiency					1583:1592	the catalytic efficiency	1569:1592	the catalytic efficiency (kcat/K m )	1569:1604	Overall, the catalytic efficiency (kcat/K m ) was highest for kaempferol-3-O-rutinoside.
29476400	10	9	theme	catalytic	1573:1581	arg1	m					1602:1602	kcat/K m	1595:1602	kcat/K m	1595:1602	Overall, the catalytic efficiency (kcat/K m ) was highest for kaempferol-3-O-rutinoside.
29476400	10	9	theme	catalytic	1573:1581	arg1	highest					1610:1616	highest	1610:1616	highest	1610:1616	Overall, the catalytic efficiency (kcat/K m ) was highest for kaempferol-3-O-rutinoside.
29476400	6	10	theme	3-O-linked	1064:1073	arg1	β-rutinosides					1085:1097	3-O-linked flavonoid β-rutinosides	1064:1097	3-O-linked flavonoid β-rutinosides	1064:1097	This enzyme is also significantly thermo-stable, with 90% activity retained after 1 h at 45 °C and 70% activity retained after 4 h, even at 50 °C. Biochemical characterization revealed that the enzyme has higher substrate specificity for 3-O-linked flavonoid β-rutinosides like rutin and kaempferol-3-O-rutinoside, than for 7-O-linked flavonoid β-rutinoside like hesperidin.
29476400	7	11	theme	polymeric	1301:1309	arg1	substrate					1321:1329	polymeric laminarin substrate	1301:1329	polymeric laminarin substrate	1301:1329	However, no activity was found with naringin, diosmin, monoglycosylated chromogenic substrates, and polymeric laminarin substrate.
29476400	2	12	theme	Aspergillus	418:428	arg1	niger					430:434	Aspergillus niger	418:434	Aspergillus niger rutinosidase	418:447	We identified a hypothetical protein (annotated as AO090012000917) encoded in the Aspergillus oryzae genome that exhibits sequence similarity with Aspergillus niger rutinosidase.
29476400	4	13	theme	peptide	716:722	arg1	processing					724:733	N-terminal signal peptide processing	698:733	N-terminal signal peptide processing	698:733	After N-deglycosylation, we observed a 42- and 40-kDa band, representing proteins before and after N-terminal signal peptide processing, respectively.
29476400	6	14	theme	7-O-linked	1150:1159	arg1	β-rutinoside					1171:1182	7-O-linked flavonoid β-rutinoside	1150:1182	7-O-linked flavonoid β-rutinoside like hesperidin	1150:1198	This enzyme is also significantly thermo-stable, with 90% activity retained after 1 h at 45 °C and 70% activity retained after 4 h, even at 50 °C. Biochemical characterization revealed that the enzyme has higher substrate specificity for 3-O-linked flavonoid β-rutinosides like rutin and kaempferol-3-O-rutinoside, than for 7-O-linked flavonoid β-rutinoside like hesperidin.
29476400	7	15	theme	laminarin	1311:1319	arg1	substrate					1321:1329	polymeric laminarin substrate	1301:1329	polymeric laminarin substrate	1301:1329	However, no activity was found with naringin, diosmin, monoglycosylated chromogenic substrates, and polymeric laminarin substrate.
29476400	6	16	theme	%	882:882	arg1	activity					884:891	90% activity	880:891	90% activity retained after 1 h at 45 °C and 70% activity retained after 4 h	880:955	This enzyme is also significantly thermo-stable, with 90% activity retained after 1 h at 45 °C and 70% activity retained after 4 h, even at 50 °C. Biochemical characterization revealed that the enzyme has higher substrate specificity for 3-O-linked flavonoid β-rutinosides like rutin and kaempferol-3-O-rutinoside, than for 7-O-linked flavonoid β-rutinoside like hesperidin.
29476400	8	17	theme	K	1361:1361	arg1	value					1365:1369	K m value	1361:1369	K m value toward rutin	1361:1382	Kinetic analyses showed that K m value toward rutin was higher than those toward hesperidin and kaempferol-3-O-rutinoside.
29476400	0	18	theme	novel	2:6	arg1	oryzae					20:25	A novel Aspergillus oryzae	0:25	A novel Aspergillus oryzae	0:25	A novel Aspergillus oryzae diglycosidase that hydrolyzes 6-O-α-L-rhamnosyl-β-D-glucoside from flavonoids.
29476400	6	19	link	7-O-linked	1150:1159	arg1	β-rutinoside					1171:1182	7-O-linked flavonoid β-rutinoside	1150:1182	7-O-linked flavonoid β-rutinoside like hesperidin	1150:1198	This enzyme is also significantly thermo-stable, with 90% activity retained after 1 h at 45 °C and 70% activity retained after 4 h, even at 50 °C. Biochemical characterization revealed that the enzyme has higher substrate specificity for 3-O-linked flavonoid β-rutinosides like rutin and kaempferol-3-O-rutinoside, than for 7-O-linked flavonoid β-rutinoside like hesperidin.
29476400	6	20	theme	90	880:881	arg1	%					882:882	%	882:882	%	882:882	This enzyme is also significantly thermo-stable, with 90% activity retained after 1 h at 45 °C and 70% activity retained after 4 h, even at 50 °C. Biochemical characterization revealed that the enzyme has higher substrate specificity for 3-O-linked flavonoid β-rutinosides like rutin and kaempferol-3-O-rutinoside, than for 7-O-linked flavonoid β-rutinoside like hesperidin.
29476400	9	21	theme	kcat	1466:1469	arg1	Meanwhile					1455:1463	Meanwhile	1455:1463	Meanwhile	1455:1463	Meanwhile, kcat value toward hesperidin was lower than those toward kaempferol-3-O-rutinoside and rutin.
29476400	9	21	theme	kcat	1466:1469	arg1	value					1471:1475	kcat value	1466:1475	kcat value toward hesperidin	1466:1493	Meanwhile, kcat value toward hesperidin was lower than those toward kaempferol-3-O-rutinoside and rutin.
29476400	8	22	theme	m	1363:1363	arg1	value					1365:1369	K m value	1361:1369	K m value toward rutin	1361:1382	Kinetic analyses showed that K m value toward rutin was higher than those toward hesperidin and kaempferol-3-O-rutinoside.
29476400	6	23	link	3-O-linked	1064:1073	arg1	β-rutinosides					1085:1097	3-O-linked flavonoid β-rutinosides	1064:1097	3-O-linked flavonoid β-rutinosides	1064:1097	This enzyme is also significantly thermo-stable, with 90% activity retained after 1 h at 45 °C and 70% activity retained after 4 h, even at 50 °C. Biochemical characterization revealed that the enzyme has higher substrate specificity for 3-O-linked flavonoid β-rutinosides like rutin and kaempferol-3-O-rutinoside, than for 7-O-linked flavonoid β-rutinoside like hesperidin.
29476400	5	24	located	observed	781:788	arg2	activity					768:775	Optimal enzymatic activity	750:775	Optimal enzymatic activity	750:775	Optimal enzymatic activity was observed at pH 4.0 and temperature of 45 °C.
29476400	5	24	located	observed	781:788	arg1	pH					793:794	pH 4.0	793:798	pH 4.0	793:798	Optimal enzymatic activity was observed at pH 4.0 and temperature of 45 °C.
29476400	5	24	located	observed	781:788	arg1	temperature					804:814	temperature	804:814	temperature of 45 °C	804:823	Optimal enzymatic activity was observed at pH 4.0 and temperature of 45 °C.
29476400	3	25	theme	recombinant	454:464	arg1	enzyme					466:471	The recombinant enzyme	450:471	The recombinant enzyme	450:471	The recombinant enzyme was expressed in Pichia pastoris GS115 and purified as a glyco-protein with apparent molecular mass of 65-75 kDa by SDS-PAGE.
29476400	3	25	theme	recombinant	454:464	arg1	glyco-protein					530:542	a glyco-protein	528:542	a glyco-protein with apparent molecular mass of 65-75 kDa by SDS-PAGE	528:596	The recombinant enzyme was expressed in Pichia pastoris GS115 and purified as a glyco-protein with apparent molecular mass of 65-75 kDa by SDS-PAGE.
29476400	4	26	theme	42-	638:640	arg1	band					653:656	a 42- and 40-kDa band	636:656	a 42- and 40-kDa band	636:656	After N-deglycosylation, we observed a 42- and 40-kDa band, representing proteins before and after N-terminal signal peptide processing, respectively.
29476400	3	27	with	glyco-protein	530:542	arg1	mass					568:571	apparent molecular mass	549:571	apparent molecular mass of 65-75 kDa by SDS-PAGE	549:596	The recombinant enzyme was expressed in Pichia pastoris GS115 and purified as a glyco-protein with apparent molecular mass of 65-75 kDa by SDS-PAGE.
29476400	7	28	gly	monoglycosylated	1256:1271	arg1	substrates					1285:1294	monoglycosylated chromogenic substrates	1256:1294	monoglycosylated chromogenic substrates	1256:1294	However, no activity was found with naringin, diosmin, monoglycosylated chromogenic substrates, and polymeric laminarin substrate.
29476400	3	29	theme	apparent	549:556	arg1	mass					568:571	apparent molecular mass	549:571	apparent molecular mass of 65-75 kDa by SDS-PAGE	549:596	The recombinant enzyme was expressed in Pichia pastoris GS115 and purified as a glyco-protein with apparent molecular mass of 65-75 kDa by SDS-PAGE.
29476400	0	30	theme	Aspergillus	8:18	arg1	oryzae					20:25	A novel Aspergillus oryzae	0:25	A novel Aspergillus oryzae	0:25	A novel Aspergillus oryzae diglycosidase that hydrolyzes 6-O-α-L-rhamnosyl-β-D-glucoside from flavonoids.
29476400	6	31	theme	substrate	1038:1046	arg1	specificity					1048:1058	higher substrate specificity	1031:1058	higher substrate specificity for 3-O-linked flavonoid β-rutinosides	1031:1097	This enzyme is also significantly thermo-stable, with 90% activity retained after 1 h at 45 °C and 70% activity retained after 4 h, even at 50 °C. Biochemical characterization revealed that the enzyme has higher substrate specificity for 3-O-linked flavonoid β-rutinosides like rutin and kaempferol-3-O-rutinoside, than for 7-O-linked flavonoid β-rutinoside like hesperidin.
29476400	2	32	with	similarity	402:411	arg1	rutinosidase					436:447	Aspergillus niger rutinosidase	418:447	Aspergillus niger rutinosidase	418:447	We identified a hypothetical protein (annotated as AO090012000917) encoded in the Aspergillus oryzae genome that exhibits sequence similarity with Aspergillus niger rutinosidase.
29476400	2	33	theme	oryzae	365:370	arg1	genome					372:377	the Aspergillus oryzae genome	349:377	the Aspergillus oryzae genome	349:377	We identified a hypothetical protein (annotated as AO090012000917) encoded in the Aspergillus oryzae genome that exhibits sequence similarity with Aspergillus niger rutinosidase.
29476400	3	34	theme	molecular	558:566	arg1	mass					568:571	apparent molecular mass	549:571	apparent molecular mass of 65-75 kDa by SDS-PAGE	549:596	The recombinant enzyme was expressed in Pichia pastoris GS115 and purified as a glyco-protein with apparent molecular mass of 65-75 kDa by SDS-PAGE.
29476400	4	35	theme	40-kDa	646:651	arg1	band					653:656	a 42- and 40-kDa band	636:656	a 42- and 40-kDa band	636:656	After N-deglycosylation, we observed a 42- and 40-kDa band, representing proteins before and after N-terminal signal peptide processing, respectively.
29476400	6	36	theme	higher	1031:1036	arg1	specificity					1048:1058	higher substrate specificity	1031:1058	higher substrate specificity for 3-O-linked flavonoid β-rutinosides	1031:1097	This enzyme is also significantly thermo-stable, with 90% activity retained after 1 h at 45 °C and 70% activity retained after 4 h, even at 50 °C. Biochemical characterization revealed that the enzyme has higher substrate specificity for 3-O-linked flavonoid β-rutinosides like rutin and kaempferol-3-O-rutinoside, than for 7-O-linked flavonoid β-rutinoside like hesperidin.
29476400	7	37	theme	monoglycosylated	1256:1271	arg1	substrates					1285:1294	monoglycosylated chromogenic substrates	1256:1294	monoglycosylated chromogenic substrates	1256:1294	However, no activity was found with naringin, diosmin, monoglycosylated chromogenic substrates, and polymeric laminarin substrate.
29476400	8	38	theme	Kinetic	1332:1338	arg1	analyses					1340:1347	Kinetic analyses	1332:1347	Kinetic analyses	1332:1347	Kinetic analyses showed that K m value toward rutin was higher than those toward hesperidin and kaempferol-3-O-rutinoside.
29476400	0	39	from	flavonoids	94:103	arg1	6-O-α-L-rhamnosyl-β-D-glucoside					57:87	6-O-α-L-rhamnosyl-β-D-glucoside	57:87	6-O-α-L-rhamnosyl-β-D-glucoside from flavonoids	57:103	A novel Aspergillus oryzae diglycosidase that hydrolyzes 6-O-α-L-rhamnosyl-β-D-glucoside from flavonoids.
29476400	7	40	theme	chromogenic	1273:1283	arg1	substrates					1285:1294	monoglycosylated chromogenic substrates	1256:1294	monoglycosylated chromogenic substrates	1256:1294	However, no activity was found with naringin, diosmin, monoglycosylated chromogenic substrates, and polymeric laminarin substrate.
29476400	2	41	dep	protein	300:306	arg1	annotated					309:317	annotated	309:317	annotated as AO090012000917	309:335	We identified a hypothetical protein (annotated as AO090012000917) encoded in the Aspergillus oryzae genome that exhibits sequence similarity with Aspergillus niger rutinosidase.
29476400	6	42	theme	%	927:927	arg1	activity					929:936	70% activity	925:936	70% activity retained after 4 h	925:955	This enzyme is also significantly thermo-stable, with 90% activity retained after 1 h at 45 °C and 70% activity retained after 4 h, even at 50 °C. Biochemical characterization revealed that the enzyme has higher substrate specificity for 3-O-linked flavonoid β-rutinosides like rutin and kaempferol-3-O-rutinoside, than for 7-O-linked flavonoid β-rutinoside like hesperidin.
29476400	2	43	theme	hypothetical	287:298	arg1	protein					300:306	a hypothetical protein	285:306	a hypothetical protein (annotated as AO090012000917) encoded in the Aspergillus oryzae genome that exhibits sequence similarity with Aspergillus niger rutinosidase	285:447	We identified a hypothetical protein (annotated as AO090012000917) encoded in the Aspergillus oryzae genome that exhibits sequence similarity with Aspergillus niger rutinosidase.
29476400	3	44	theme	65-75 kDa	576:584	arg1	mass					568:571	apparent molecular mass	549:571	apparent molecular mass of 65-75 kDa by SDS-PAGE	549:596	The recombinant enzyme was expressed in Pichia pastoris GS115 and purified as a glyco-protein with apparent molecular mass of 65-75 kDa by SDS-PAGE.
29476400	3	45	theme	Pichia	490:495	arg1	pastoris					497:504	Pichia pastoris GS115	490:510	Pichia pastoris GS115	490:510	The recombinant enzyme was expressed in Pichia pastoris GS115 and purified as a glyco-protein with apparent molecular mass of 65-75 kDa by SDS-PAGE.
29476400	6	46	theme	70	925:926	arg1	%					927:927	%	927:927	%	927:927	This enzyme is also significantly thermo-stable, with 90% activity retained after 1 h at 45 °C and 70% activity retained after 4 h, even at 50 °C. Biochemical characterization revealed that the enzyme has higher substrate specificity for 3-O-linked flavonoid β-rutinosides like rutin and kaempferol-3-O-rutinoside, than for 7-O-linked flavonoid β-rutinoside like hesperidin.
29476400	1	47	theme	glycosidic	166:175	arg1	linkage					177:183	the glycosidic linkage	162:183	the glycosidic linkage between the disaccharide 6-O-α-L-rhamnosyl-β-D-glucoside (rutinose) and the aglycone	162:268	α-L-Rhamnosyl-β-D-glucosidase (rutinosidase) hydrolyzes the glycosidic linkage between the disaccharide 6-O-α-L-rhamnosyl-β-D-glucoside (rutinose) and the aglycone.
29476400	2	48	theme	sequence	393:400	arg1	similarity					402:411	sequence similarity	393:411	sequence similarity with Aspergillus niger rutinosidase	393:447	We identified a hypothetical protein (annotated as AO090012000917) encoded in the Aspergillus oryzae genome that exhibits sequence similarity with Aspergillus niger rutinosidase.
29476400	6	49	theme	flavonoid	1161:1169	arg1	β-rutinoside					1171:1182	7-O-linked flavonoid β-rutinoside	1150:1182	7-O-linked flavonoid β-rutinoside like hesperidin	1150:1198	This enzyme is also significantly thermo-stable, with 90% activity retained after 1 h at 45 °C and 70% activity retained after 4 h, even at 50 °C. Biochemical characterization revealed that the enzyme has higher substrate specificity for 3-O-linked flavonoid β-rutinosides like rutin and kaempferol-3-O-rutinoside, than for 7-O-linked flavonoid β-rutinoside like hesperidin.
29476400	4	50	theme	signal	709:714	arg1	processing					724:733	N-terminal signal peptide processing	698:733	N-terminal signal peptide processing	698:733	After N-deglycosylation, we observed a 42- and 40-kDa band, representing proteins before and after N-terminal signal peptide processing, respectively.
29476400	6	51	from	50 °C.	966:971	arg1	thermo-stable					860:872	thermo-stable	860:872	thermo-stable	860:872	This enzyme is also significantly thermo-stable, with 90% activity retained after 1 h at 45 °C and 70% activity retained after 4 h, even at 50 °C. Biochemical characterization revealed that the enzyme has higher substrate specificity for 3-O-linked flavonoid β-rutinosides like rutin and kaempferol-3-O-rutinoside, than for 7-O-linked flavonoid β-rutinoside like hesperidin.
29476400	6	52	contain	has	1027:1029	arg1	enzyme					1020:1025	the enzyme	1016:1025	the enzyme	1016:1025	This enzyme is also significantly thermo-stable, with 90% activity retained after 1 h at 45 °C and 70% activity retained after 4 h, even at 50 °C. Biochemical characterization revealed that the enzyme has higher substrate specificity for 3-O-linked flavonoid β-rutinosides like rutin and kaempferol-3-O-rutinoside, than for 7-O-linked flavonoid β-rutinoside like hesperidin.
29476400	6	52	contain	has	1027:1029	arg2	specificity					1048:1058	higher substrate specificity	1031:1058	higher substrate specificity for 3-O-linked flavonoid β-rutinosides	1031:1097	This enzyme is also significantly thermo-stable, with 90% activity retained after 1 h at 45 °C and 70% activity retained after 4 h, even at 50 °C. Biochemical characterization revealed that the enzyme has higher substrate specificity for 3-O-linked flavonoid β-rutinosides like rutin and kaempferol-3-O-rutinoside, than for 7-O-linked flavonoid β-rutinoside like hesperidin.
29476400	1	53	theme	disaccharide	197:208	arg1	rutinose					243:250	rutinose	243:250	rutinose	243:250	α-L-Rhamnosyl-β-D-glucosidase (rutinosidase) hydrolyzes the glycosidic linkage between the disaccharide 6-O-α-L-rhamnosyl-β-D-glucoside (rutinose) and the aglycone.
29476400	1	53	theme	disaccharide	197:208	arg1	6-O-α-L-rhamnosyl-β-D-glucoside					210:240	the disaccharide 6-O-α-L-rhamnosyl-β-D-glucoside	193:240	the disaccharide 6-O-α-L-rhamnosyl-β-D-glucoside (rutinose)	193:251	α-L-Rhamnosyl-β-D-glucosidase (rutinosidase) hydrolyzes the glycosidic linkage between the disaccharide 6-O-α-L-rhamnosyl-β-D-glucoside (rutinose) and the aglycone.
29476400	4	54	theme	N-terminal	698:707	arg1	processing					724:733	N-terminal signal peptide processing	698:733	N-terminal signal peptide processing	698:733	After N-deglycosylation, we observed a 42- and 40-kDa band, representing proteins before and after N-terminal signal peptide processing, respectively.
29365334	0	0	theme	Signaling	79:87	arg1	Pathway					89:95	the TLR4 Signaling Pathway	70:95	the TLR4 Signaling Pathway	70:95	Berberine Modulates Gut Microbiota and Reduces Insulin Resistance via the TLR4 Signaling Pathway.
29365334	2	1	theme	factor	579:584	arg1	pathway					604:610	the lipopolysaccharide (LPS)/toll-like receptor 4 (TLR4)/tumor necrosis factor (TNF)-α signaling pathway	507:610	the lipopolysaccharide (LPS)/toll-like receptor 4 (TLR4)/tumor necrosis factor (TNF)-α signaling pathway in livers of HFD-fed obese rats	507:642	We investigated the effects of berberine on obesity and insulin resistance by examining the lipopolysaccharide (LPS)/toll-like receptor 4 (TLR4)/tumor necrosis factor (TNF)-α signaling pathway in livers of HFD-fed obese rats.
29365334	4	2	theme	significant	875:885	arg1	effects					887:893	no significant effects	872:893	no significant effects on body weight, visceral fat mass or the visceral fat to body weight ratio	872:968	However, berberine had no significant effects on body weight, visceral fat mass or the visceral fat to body weight ratio.
29365334	1	3	theme	Chinese	151:157	arg1	chinensis					182:190	chinensis	182:190	chinensis	182:190	Berberine, a natural compound extracted from several Chinese herbs including Coptis chinensis, has been shown to have anti-obesity effects and prevents insulin resistance in high-fat diet (HFD)-fed obese rats by modulating the gut microbiota; however, the molecular mechanisms underlying these activities remain unknown.
29365334	1	3	theme	Chinese	151:157	arg1	herbs					159:163	several Chinese herbs	143:163	several Chinese herbs including Coptis chinensis	143:190	Berberine, a natural compound extracted from several Chinese herbs including Coptis chinensis, has been shown to have anti-obesity effects and prevents insulin resistance in high-fat diet (HFD)-fed obese rats by modulating the gut microbiota; however, the molecular mechanisms underlying these activities remain unknown.
29365334	3	4	theme	insulin	807:813	arg1	resistance					815:824	insulin resistance	807:824	insulin resistance	807:824	Our results showed that 8-week berberine (200 mg/kg) treatment significantly reduced fasting blood glucose, triglyceride, low-density lipoprotein-cholesterol and insulin resistance in HFD-fed obese rats.
29365334	0	5	theme	TLR4	74:77	arg1	Pathway					89:95	the TLR4 Signaling Pathway	70:95	the TLR4 Signaling Pathway	70:95	Berberine Modulates Gut Microbiota and Reduces Insulin Resistance via the TLR4 Signaling Pathway.
29365334	2	6	theme	necrosis	570:577	arg1	TNF					587:589	TNF	587:589	TNF	587:589	We investigated the effects of berberine on obesity and insulin resistance by examining the lipopolysaccharide (LPS)/toll-like receptor 4 (TLR4)/tumor necrosis factor (TNF)-α signaling pathway in livers of HFD-fed obese rats.
29365334	2	6	theme	necrosis	570:577	arg1	factor					579:584	the lipopolysaccharide (LPS)/toll-like receptor 4 (TLR4)/tumor necrosis factor	507:584	the lipopolysaccharide (LPS)/toll-like receptor 4 (TLR4)/tumor necrosis factor (TNF)-α signaling pathway in livers of HFD-fed obese rats	507:642	We investigated the effects of berberine on obesity and insulin resistance by examining the lipopolysaccharide (LPS)/toll-like receptor 4 (TLR4)/tumor necrosis factor (TNF)-α signaling pathway in livers of HFD-fed obese rats.
29365334	6	7	theme	gut	1056:1058	arg1	composition					1071:1081	the gut microbiota composition	1052:1081	the gut microbiota composition	1052:1081	A prolonged HFD altered the gut microbiota composition by reducing protective bacteria like Bifidobacterium and increasing gram negative bacteria like Escherichia coli, which resulted in increased LPS release into plasma.
29365334	1	8	theme	insulin	250:256	arg1	resistance					258:267	insulin resistance	250:267	insulin resistance in high-fat diet (HFD)-fed obese rats	250:305	Berberine, a natural compound extracted from several Chinese herbs including Coptis chinensis, has been shown to have anti-obesity effects and prevents insulin resistance in high-fat diet (HFD)-fed obese rats by modulating the gut microbiota; however, the molecular mechanisms underlying these activities remain unknown.
29365334	4	9	theme	fat	920:922	arg1	mass					924:927	visceral fat mass	911:927	visceral fat mass	911:927	However, berberine had no significant effects on body weight, visceral fat mass or the visceral fat to body weight ratio.
29365334	1	10	theme	gut	325:327	arg1	microbiota					329:338	the gut microbiota	321:338	the gut microbiota	321:338	Berberine, a natural compound extracted from several Chinese herbs including Coptis chinensis, has been shown to have anti-obesity effects and prevents insulin resistance in high-fat diet (HFD)-fed obese rats by modulating the gut microbiota; however, the molecular mechanisms underlying these activities remain unknown.
29365334	2	11	theme	/tumor	563:568	arg1	TNF					587:589	TNF	587:589	TNF	587:589	We investigated the effects of berberine on obesity and insulin resistance by examining the lipopolysaccharide (LPS)/toll-like receptor 4 (TLR4)/tumor necrosis factor (TNF)-α signaling pathway in livers of HFD-fed obese rats.
29365334	2	11	theme	/tumor	563:568	arg1	factor					579:584	the lipopolysaccharide (LPS)/toll-like receptor 4 (TLR4)/tumor necrosis factor	507:584	the lipopolysaccharide (LPS)/toll-like receptor 4 (TLR4)/tumor necrosis factor (TNF)-α signaling pathway in livers of HFD-fed obese rats	507:642	We investigated the effects of berberine on obesity and insulin resistance by examining the lipopolysaccharide (LPS)/toll-like receptor 4 (TLR4)/tumor necrosis factor (TNF)-α signaling pathway in livers of HFD-fed obese rats.
29365334	6	12	theme	negative	1156:1163	arg1	bacteria					1165:1172	gram negative bacteria	1151:1172	gram negative bacteria like Escherichia coli	1151:1194	A prolonged HFD altered the gut microbiota composition by reducing protective bacteria like Bifidobacterium and increasing gram negative bacteria like Escherichia coli, which resulted in increased LPS release into plasma.
29365334	4	13	theme	visceral	911:918	arg1	mass					924:927	visceral fat mass	911:927	visceral fat mass	911:927	However, berberine had no significant effects on body weight, visceral fat mass or the visceral fat to body weight ratio.
29365334	3	14	theme	berberine	676:684	arg1	treatment					698:706	8-week berberine (200 mg/kg) treatment	669:706	8-week berberine (200 mg/kg) treatment	669:706	Our results showed that 8-week berberine (200 mg/kg) treatment significantly reduced fasting blood glucose, triglyceride, low-density lipoprotein-cholesterol and insulin resistance in HFD-fed obese rats.
29365334	3	15	from	glucose	744:750	arg1	rats					843:846	HFD-fed obese rats	829:846	HFD-fed obese rats	829:846	Our results showed that 8-week berberine (200 mg/kg) treatment significantly reduced fasting blood glucose, triglyceride, low-density lipoprotein-cholesterol and insulin resistance in HFD-fed obese rats.
29365334	6	16	theme	microbiota	1060:1069	arg1	composition					1071:1081	the gut microbiota composition	1052:1081	the gut microbiota composition	1052:1081	A prolonged HFD altered the gut microbiota composition by reducing protective bacteria like Bifidobacterium and increasing gram negative bacteria like Escherichia coli, which resulted in increased LPS release into plasma.
29365334	4	17	theme	body	952:955	arg1	ratio					964:968	body weight ratio	952:968	body weight ratio	952:968	However, berberine had no significant effects on body weight, visceral fat mass or the visceral fat to body weight ratio.
29365334	2	18	from	effects	439:445	arg1	obesity					463:469	obesity	463:469	obesity	463:469	We investigated the effects of berberine on obesity and insulin resistance by examining the lipopolysaccharide (LPS)/toll-like receptor 4 (TLR4)/tumor necrosis factor (TNF)-α signaling pathway in livers of HFD-fed obese rats.
29365334	2	18	from	effects	439:445	arg1	resistance					483:492	insulin resistance	475:492	insulin resistance	475:492	We investigated the effects of berberine on obesity and insulin resistance by examining the lipopolysaccharide (LPS)/toll-like receptor 4 (TLR4)/tumor necrosis factor (TNF)-α signaling pathway in livers of HFD-fed obese rats.
29365334	2	19	theme	-α	591:592	arg1	pathway					604:610	the lipopolysaccharide (LPS)/toll-like receptor 4 (TLR4)/tumor necrosis factor (TNF)-α signaling pathway	507:610	the lipopolysaccharide (LPS)/toll-like receptor 4 (TLR4)/tumor necrosis factor (TNF)-α signaling pathway in livers of HFD-fed obese rats	507:642	We investigated the effects of berberine on obesity and insulin resistance by examining the lipopolysaccharide (LPS)/toll-like receptor 4 (TLR4)/tumor necrosis factor (TNF)-α signaling pathway in livers of HFD-fed obese rats.
29365334	2	20	theme	signaling	594:602	arg1	pathway					604:610	the lipopolysaccharide (LPS)/toll-like receptor 4 (TLR4)/tumor necrosis factor (TNF)-α signaling pathway	507:610	the lipopolysaccharide (LPS)/toll-like receptor 4 (TLR4)/tumor necrosis factor (TNF)-α signaling pathway in livers of HFD-fed obese rats	507:642	We investigated the effects of berberine on obesity and insulin resistance by examining the lipopolysaccharide (LPS)/toll-like receptor 4 (TLR4)/tumor necrosis factor (TNF)-α signaling pathway in livers of HFD-fed obese rats.
29365334	8	21	theme	gut	1536:1538	arg1	microbiota					1540:1549	the gut microbiota	1532:1549	the gut microbiota	1532:1549	These findings suggested that berberine may reduce insulin resistance, at least in part by modulating the gut microbiota along with inhibiting LPS/TLR4/TNF-α signaling in the liver.
29365334	2	22	theme	rats	639:642	arg1	livers					615:620	livers	615:620	livers of HFD-fed obese rats	615:642	We investigated the effects of berberine on obesity and insulin resistance by examining the lipopolysaccharide (LPS)/toll-like receptor 4 (TLR4)/tumor necrosis factor (TNF)-α signaling pathway in livers of HFD-fed obese rats.
29365334	3	23	theme	low-density	767:777	arg1	lipoprotein-cholesterol					779:801	low-density lipoprotein-cholesterol	767:801	low-density lipoprotein-cholesterol	767:801	Our results showed that 8-week berberine (200 mg/kg) treatment significantly reduced fasting blood glucose, triglyceride, low-density lipoprotein-cholesterol and insulin resistance in HFD-fed obese rats.
29365334	4	24	contain	had	868:870	arg2	effects					887:893	no significant effects	872:893	no significant effects on body weight, visceral fat mass or the visceral fat to body weight ratio	872:968	However, berberine had no significant effects on body weight, visceral fat mass or the visceral fat to body weight ratio.
29365334	4	24	contain	had	868:870	arg1	berberine					858:866	berberine	858:866	berberine	858:866	However, berberine had no significant effects on body weight, visceral fat mass or the visceral fat to body weight ratio.
29365334	7	25	theme	increased	1345:1353	arg1	expression					1405:1414	increased insulin receptor and insulin receptor substrate-1 expression	1345:1414	increased insulin receptor and insulin receptor substrate-1 expression in the liver	1345:1427	Berberine reversed these effects and inhibited LPS-induced TLR4/TNF-α activation, resulting in increased insulin receptor and insulin receptor substrate-1 expression in the liver.
29365334	3	26	theme	8-week	669:674	arg1	treatment					698:706	8-week berberine (200 mg/kg) treatment	669:706	8-week berberine (200 mg/kg) treatment	669:706	Our results showed that 8-week berberine (200 mg/kg) treatment significantly reduced fasting blood glucose, triglyceride, low-density lipoprotein-cholesterol and insulin resistance in HFD-fed obese rats.
29365334	2	27	theme	obese	633:637	arg1	rats					639:642	HFD-fed obese rats	625:642	HFD-fed obese rats	625:642	We investigated the effects of berberine on obesity and insulin resistance by examining the lipopolysaccharide (LPS)/toll-like receptor 4 (TLR4)/tumor necrosis factor (TNF)-α signaling pathway in livers of HFD-fed obese rats.
29365334	6	28	theme	gram	1151:1154	arg1	bacteria					1165:1172	gram negative bacteria	1151:1172	gram negative bacteria like Escherichia coli	1151:1194	A prolonged HFD altered the gut microbiota composition by reducing protective bacteria like Bifidobacterium and increasing gram negative bacteria like Escherichia coli, which resulted in increased LPS release into plasma.
29365334	3	29	from	triglyceride	753:764	arg1	rats					843:846	HFD-fed obese rats	829:846	HFD-fed obese rats	829:846	Our results showed that 8-week berberine (200 mg/kg) treatment significantly reduced fasting blood glucose, triglyceride, low-density lipoprotein-cholesterol and insulin resistance in HFD-fed obese rats.
29365334	1	30	theme	high-fat	272:279	arg1	HFD					287:289	HFD	287:289	HFD	287:289	Berberine, a natural compound extracted from several Chinese herbs including Coptis chinensis, has been shown to have anti-obesity effects and prevents insulin resistance in high-fat diet (HFD)-fed obese rats by modulating the gut microbiota; however, the molecular mechanisms underlying these activities remain unknown.
29365334	1	30	theme	high-fat	272:279	arg1	diet					281:284	high-fat diet	272:284	high-fat diet (HFD)-fed obese rats	272:305	Berberine, a natural compound extracted from several Chinese herbs including Coptis chinensis, has been shown to have anti-obesity effects and prevents insulin resistance in high-fat diet (HFD)-fed obese rats by modulating the gut microbiota; however, the molecular mechanisms underlying these activities remain unknown.
29365334	2	31	theme	HFD-fed	625:631	arg1	rats					639:642	HFD-fed obese rats	625:642	HFD-fed obese rats	625:642	We investigated the effects of berberine on obesity and insulin resistance by examining the lipopolysaccharide (LPS)/toll-like receptor 4 (TLR4)/tumor necrosis factor (TNF)-α signaling pathway in livers of HFD-fed obese rats.
29365334	5	32	theme	hepatic	1009:1015	arg1	steatosis					1017:1025	HFD-induced hepatic steatosis	997:1025	HFD-induced hepatic steatosis	997:1025	Berberine also attenuated HFD-induced hepatic steatosis.
29365334	6	33	theme	prolonged	1030:1038	arg1	HFD					1040:1042	A prolonged HFD	1028:1042	A prolonged HFD	1028:1042	A prolonged HFD altered the gut microbiota composition by reducing protective bacteria like Bifidobacterium and increasing gram negative bacteria like Escherichia coli, which resulted in increased LPS release into plasma.
29365334	7	34	theme	receptor	1384:1391	arg1	substrate-1					1393:1403	insulin receptor substrate-1	1376:1403	insulin receptor substrate-1	1376:1403	Berberine reversed these effects and inhibited LPS-induced TLR4/TNF-α activation, resulting in increased insulin receptor and insulin receptor substrate-1 expression in the liver.
29365334	1	35	theme	diet	281:284	arg1	rats					302:305	high-fat diet (HFD)-fed obese rats	272:305	high-fat diet (HFD)-fed obese rats	272:305	Berberine, a natural compound extracted from several Chinese herbs including Coptis chinensis, has been shown to have anti-obesity effects and prevents insulin resistance in high-fat diet (HFD)-fed obese rats by modulating the gut microbiota; however, the molecular mechanisms underlying these activities remain unknown.
29365334	1	36	from	resistance	258:267	arg1	rats					302:305	high-fat diet (HFD)-fed obese rats	272:305	high-fat diet (HFD)-fed obese rats	272:305	Berberine, a natural compound extracted from several Chinese herbs including Coptis chinensis, has been shown to have anti-obesity effects and prevents insulin resistance in high-fat diet (HFD)-fed obese rats by modulating the gut microbiota; however, the molecular mechanisms underlying these activities remain unknown.
29365334	5	37	theme	HFD-induced	997:1007	arg1	steatosis					1017:1025	HFD-induced hepatic steatosis	997:1025	HFD-induced hepatic steatosis	997:1025	Berberine also attenuated HFD-induced hepatic steatosis.
29365334	7	38	theme	TLR4/TNF-α	1309:1318	arg1	activation					1320:1329	LPS-induced TLR4/TNF-α activation	1297:1329	LPS-induced TLR4/TNF-α activation	1297:1329	Berberine reversed these effects and inhibited LPS-induced TLR4/TNF-α activation, resulting in increased insulin receptor and insulin receptor substrate-1 expression in the liver.
29365334	0	39	theme	Gut	20:22	arg1	Microbiota					24:33	Gut Microbiota	20:33	Gut Microbiota	20:33	Berberine Modulates Gut Microbiota and Reduces Insulin Resistance via the TLR4 Signaling Pathway.
29365334	3	40	theme	200 mg/kg	687:695	arg1	treatment					698:706	8-week berberine (200 mg/kg) treatment	669:706	8-week berberine (200 mg/kg) treatment	669:706	Our results showed that 8-week berberine (200 mg/kg) treatment significantly reduced fasting blood glucose, triglyceride, low-density lipoprotein-cholesterol and insulin resistance in HFD-fed obese rats.
29365334	7	41	from	expression	1405:1414	arg1	liver					1423:1427	the liver	1419:1427	the liver	1419:1427	Berberine reversed these effects and inhibited LPS-induced TLR4/TNF-α activation, resulting in increased insulin receptor and insulin receptor substrate-1 expression in the liver.
29365334	4	42	theme	weight	957:962	arg1	ratio					964:968	body weight ratio	952:968	body weight ratio	952:968	However, berberine had no significant effects on body weight, visceral fat mass or the visceral fat to body weight ratio.
29365334	1	43	theme	molecular	354:362	arg1	mechanisms					364:373	the molecular mechanisms	350:373	the molecular mechanisms underlying these activities	350:401	Berberine, a natural compound extracted from several Chinese herbs including Coptis chinensis, has been shown to have anti-obesity effects and prevents insulin resistance in high-fat diet (HFD)-fed obese rats by modulating the gut microbiota; however, the molecular mechanisms underlying these activities remain unknown.
29365334	6	44	dep	increasing	1140:1149	arg1	resulted					1203:1210	resulted	1203:1210	resulted in increased LPS release into plasma	1203:1247	A prolonged HFD altered the gut microbiota composition by reducing protective bacteria like Bifidobacterium and increasing gram negative bacteria like Escherichia coli, which resulted in increased LPS release into plasma.
29365334	2	45	theme	berberine	450:458	arg1	effects					439:445	the effects	435:445	the effects of berberine on obesity and insulin resistance	435:492	We investigated the effects of berberine on obesity and insulin resistance by examining the lipopolysaccharide (LPS)/toll-like receptor 4 (TLR4)/tumor necrosis factor (TNF)-α signaling pathway in livers of HFD-fed obese rats.
29365334	3	46	theme	blood	738:742	arg1	glucose					744:750	fasting blood glucose	730:750	fasting blood glucose	730:750	Our results showed that 8-week berberine (200 mg/kg) treatment significantly reduced fasting blood glucose, triglyceride, low-density lipoprotein-cholesterol and insulin resistance in HFD-fed obese rats.
29365334	3	47	from	resistance	815:824	arg1	rats					843:846	HFD-fed obese rats	829:846	HFD-fed obese rats	829:846	Our results showed that 8-week berberine (200 mg/kg) treatment significantly reduced fasting blood glucose, triglyceride, low-density lipoprotein-cholesterol and insulin resistance in HFD-fed obese rats.
29365334	6	48	theme	LPS	1225:1227	arg1	release					1229:1235	increased LPS release	1215:1235	increased LPS release into plasma	1215:1247	A prolonged HFD altered the gut microbiota composition by reducing protective bacteria like Bifidobacterium and increasing gram negative bacteria like Escherichia coli, which resulted in increased LPS release into plasma.
29365334	7	49	theme	substrate-1	1393:1403	arg1	expression					1405:1414	increased insulin receptor and insulin receptor substrate-1 expression	1345:1414	increased insulin receptor and insulin receptor substrate-1 expression in the liver	1345:1427	Berberine reversed these effects and inhibited LPS-induced TLR4/TNF-α activation, resulting in increased insulin receptor and insulin receptor substrate-1 expression in the liver.
29365334	7	50	theme	LPS-induced	1297:1307	arg1	activation					1320:1329	LPS-induced TLR4/TNF-α activation	1297:1329	LPS-induced TLR4/TNF-α activation	1297:1329	Berberine reversed these effects and inhibited LPS-induced TLR4/TNF-α activation, resulting in increased insulin receptor and insulin receptor substrate-1 expression in the liver.
29365334	1	51	theme	-fed	291:294	arg1	rats					302:305	high-fat diet (HFD)-fed obese rats	272:305	high-fat diet (HFD)-fed obese rats	272:305	Berberine, a natural compound extracted from several Chinese herbs including Coptis chinensis, has been shown to have anti-obesity effects and prevents insulin resistance in high-fat diet (HFD)-fed obese rats by modulating the gut microbiota; however, the molecular mechanisms underlying these activities remain unknown.
29365334	8	52	theme	insulin	1481:1487	arg1	resistance					1489:1498	insulin resistance	1481:1498	insulin resistance	1481:1498	These findings suggested that berberine may reduce insulin resistance, at least in part by modulating the gut microbiota along with inhibiting LPS/TLR4/TNF-α signaling in the liver.
29365334	6	53	theme	increased	1215:1223	arg1	release					1229:1235	increased LPS release	1215:1235	increased LPS release into plasma	1215:1247	A prolonged HFD altered the gut microbiota composition by reducing protective bacteria like Bifidobacterium and increasing gram negative bacteria like Escherichia coli, which resulted in increased LPS release into plasma.
29365334	2	54	from	pathway	604:610	arg1	livers					615:620	livers	615:620	livers of HFD-fed obese rats	615:642	We investigated the effects of berberine on obesity and insulin resistance by examining the lipopolysaccharide (LPS)/toll-like receptor 4 (TLR4)/tumor necrosis factor (TNF)-α signaling pathway in livers of HFD-fed obese rats.
29365334	0	55	theme	Insulin	47:53	arg1	Resistance					55:64	Insulin Resistance	47:64	Insulin Resistance	47:64	Berberine Modulates Gut Microbiota and Reduces Insulin Resistance via the TLR4 Signaling Pathway.
29365334	1	56	theme	obese	296:300	arg1	rats					302:305	high-fat diet (HFD)-fed obese rats	272:305	high-fat diet (HFD)-fed obese rats	272:305	Berberine, a natural compound extracted from several Chinese herbs including Coptis chinensis, has been shown to have anti-obesity effects and prevents insulin resistance in high-fat diet (HFD)-fed obese rats by modulating the gut microbiota; however, the molecular mechanisms underlying these activities remain unknown.
29365334	2	57	theme	/toll-like	535:544	arg1	receptor					546:553	the lipopolysaccharide (LPS)/toll-like receptor 4	507:555	the lipopolysaccharide (LPS)/toll-like receptor 4 (TLR4)/tumor necrosis factor (TNF)-α signaling pathway in livers of HFD-fed obese rats	507:642	We investigated the effects of berberine on obesity and insulin resistance by examining the lipopolysaccharide (LPS)/toll-like receptor 4 (TLR4)/tumor necrosis factor (TNF)-α signaling pathway in livers of HFD-fed obese rats.
29365334	2	57	theme	/toll-like	535:544	arg1	TLR4					558:561	TLR4	558:561	TLR4	558:561	We investigated the effects of berberine on obesity and insulin resistance by examining the lipopolysaccharide (LPS)/toll-like receptor 4 (TLR4)/tumor necrosis factor (TNF)-α signaling pathway in livers of HFD-fed obese rats.
29365334	4	58	from	effects	887:893	arg1	mass					924:927	visceral fat mass	911:927	visceral fat mass	911:927	However, berberine had no significant effects on body weight, visceral fat mass or the visceral fat to body weight ratio.
29365334	4	58	from	effects	887:893	arg1	weight					903:908	body weight	898:908	body weight	898:908	However, berberine had no significant effects on body weight, visceral fat mass or the visceral fat to body weight ratio.
29365334	4	58	from	effects	887:893	arg1	fat					945:947	the visceral fat	932:947	the visceral fat	932:947	However, berberine had no significant effects on body weight, visceral fat mass or the visceral fat to body weight ratio.
29365334	1	59	contain	have	211:214	arg1	compound					119:126	a natural compound	109:126	a natural compound extracted from several Chinese herbs including Coptis chinensis	109:190	Berberine, a natural compound extracted from several Chinese herbs including Coptis chinensis, has been shown to have anti-obesity effects and prevents insulin resistance in high-fat diet (HFD)-fed obese rats by modulating the gut microbiota; however, the molecular mechanisms underlying these activities remain unknown.
29365334	1	59	contain	have	211:214	arg1	Berberine					98:106	Berberine	98:106	Berberine	98:106	Berberine, a natural compound extracted from several Chinese herbs including Coptis chinensis, has been shown to have anti-obesity effects and prevents insulin resistance in high-fat diet (HFD)-fed obese rats by modulating the gut microbiota; however, the molecular mechanisms underlying these activities remain unknown.
29365334	1	59	contain	have	211:214	arg2	effects					229:235	anti-obesity effects	216:235	anti-obesity effects	216:235	Berberine, a natural compound extracted from several Chinese herbs including Coptis chinensis, has been shown to have anti-obesity effects and prevents insulin resistance in high-fat diet (HFD)-fed obese rats by modulating the gut microbiota; however, the molecular mechanisms underlying these activities remain unknown.
29365334	1	60	theme	natural	111:117	arg1	compound					119:126	a natural compound	109:126	a natural compound extracted from several Chinese herbs including Coptis chinensis	109:190	Berberine, a natural compound extracted from several Chinese herbs including Coptis chinensis, has been shown to have anti-obesity effects and prevents insulin resistance in high-fat diet (HFD)-fed obese rats by modulating the gut microbiota; however, the molecular mechanisms underlying these activities remain unknown.
29365334	1	60	theme	natural	111:117	arg1	Berberine					98:106	Berberine	98:106	Berberine	98:106	Berberine, a natural compound extracted from several Chinese herbs including Coptis chinensis, has been shown to have anti-obesity effects and prevents insulin resistance in high-fat diet (HFD)-fed obese rats by modulating the gut microbiota; however, the molecular mechanisms underlying these activities remain unknown.
29365334	3	61	theme	obese	837:841	arg1	rats					843:846	HFD-fed obese rats	829:846	HFD-fed obese rats	829:846	Our results showed that 8-week berberine (200 mg/kg) treatment significantly reduced fasting blood glucose, triglyceride, low-density lipoprotein-cholesterol and insulin resistance in HFD-fed obese rats.
29365334	3	62	theme	fasting	730:736	arg1	glucose					744:750	fasting blood glucose	730:750	fasting blood glucose	730:750	Our results showed that 8-week berberine (200 mg/kg) treatment significantly reduced fasting blood glucose, triglyceride, low-density lipoprotein-cholesterol and insulin resistance in HFD-fed obese rats.
29365334	8	63	from	signaling	1588:1596	arg1	liver					1605:1609	the liver	1601:1609	the liver	1601:1609	These findings suggested that berberine may reduce insulin resistance, at least in part by modulating the gut microbiota along with inhibiting LPS/TLR4/TNF-α signaling in the liver.
29365334	8	64	theme	LPS/TLR4/TNF-α	1573:1586	arg1	signaling					1588:1596	LPS/TLR4/TNF-α signaling	1573:1596	LPS/TLR4/TNF-α signaling in the liver	1573:1609	These findings suggested that berberine may reduce insulin resistance, at least in part by modulating the gut microbiota along with inhibiting LPS/TLR4/TNF-α signaling in the liver.
29365334	6	65	theme	protective	1095:1104	arg1	bacteria					1106:1113	protective bacteria	1095:1113	protective bacteria like Bifidobacterium	1095:1134	A prolonged HFD altered the gut microbiota composition by reducing protective bacteria like Bifidobacterium and increasing gram negative bacteria like Escherichia coli, which resulted in increased LPS release into plasma.
29365334	2	66	theme	receptor	546:553	arg1	TNF					587:589	TNF	587:589	TNF	587:589	We investigated the effects of berberine on obesity and insulin resistance by examining the lipopolysaccharide (LPS)/toll-like receptor 4 (TLR4)/tumor necrosis factor (TNF)-α signaling pathway in livers of HFD-fed obese rats.
29365334	2	66	theme	receptor	546:553	arg1	factor					579:584	the lipopolysaccharide (LPS)/toll-like receptor 4 (TLR4)/tumor necrosis factor	507:584	the lipopolysaccharide (LPS)/toll-like receptor 4 (TLR4)/tumor necrosis factor (TNF)-α signaling pathway in livers of HFD-fed obese rats	507:642	We investigated the effects of berberine on obesity and insulin resistance by examining the lipopolysaccharide (LPS)/toll-like receptor 4 (TLR4)/tumor necrosis factor (TNF)-α signaling pathway in livers of HFD-fed obese rats.
29365334	7	67	theme	insulin	1376:1382	arg1	substrate-1					1393:1403	insulin receptor substrate-1	1376:1403	insulin receptor substrate-1	1376:1403	Berberine reversed these effects and inhibited LPS-induced TLR4/TNF-α activation, resulting in increased insulin receptor and insulin receptor substrate-1 expression in the liver.
29365334	1	68	theme	anti-obesity	216:227	arg1	effects					229:235	anti-obesity effects	216:235	anti-obesity effects	216:235	Berberine, a natural compound extracted from several Chinese herbs including Coptis chinensis, has been shown to have anti-obesity effects and prevents insulin resistance in high-fat diet (HFD)-fed obese rats by modulating the gut microbiota; however, the molecular mechanisms underlying these activities remain unknown.
29365334	4	69	theme	visceral	936:943	arg1	fat					945:947	the visceral fat	932:947	the visceral fat	932:947	However, berberine had no significant effects on body weight, visceral fat mass or the visceral fat to body weight ratio.
29365334	4	70	theme	body	898:901	arg1	weight					903:908	body weight	898:908	body weight	898:908	However, berberine had no significant effects on body weight, visceral fat mass or the visceral fat to body weight ratio.
29365334	7	71	theme	insulin	1355:1361	arg1	receptor					1363:1370	insulin receptor	1355:1370	insulin receptor	1355:1370	Berberine reversed these effects and inhibited LPS-induced TLR4/TNF-α activation, resulting in increased insulin receptor and insulin receptor substrate-1 expression in the liver.
29365334	3	72	theme	HFD-fed	829:835	arg1	rats					843:846	HFD-fed obese rats	829:846	HFD-fed obese rats	829:846	Our results showed that 8-week berberine (200 mg/kg) treatment significantly reduced fasting blood glucose, triglyceride, low-density lipoprotein-cholesterol and insulin resistance in HFD-fed obese rats.
29365334	2	73	theme	insulin	475:481	arg1	resistance					483:492	insulin resistance	475:492	insulin resistance	475:492	We investigated the effects of berberine on obesity and insulin resistance by examining the lipopolysaccharide (LPS)/toll-like receptor 4 (TLR4)/tumor necrosis factor (TNF)-α signaling pathway in livers of HFD-fed obese rats.
29365334	7	74	theme	receptor	1363:1370	arg1	expression					1405:1414	increased insulin receptor and insulin receptor substrate-1 expression	1345:1414	increased insulin receptor and insulin receptor substrate-1 expression in the liver	1345:1427	Berberine reversed these effects and inhibited LPS-induced TLR4/TNF-α activation, resulting in increased insulin receptor and insulin receptor substrate-1 expression in the liver.
29365334	8	75	dep	modulating	1521:1530	arg1	inhibiting					1562:1571	inhibiting	1562:1571	inhibiting LPS/TLR4/TNF-α signaling in the liver	1562:1609	These findings suggested that berberine may reduce insulin resistance, at least in part by modulating the gut microbiota along with inhibiting LPS/TLR4/TNF-α signaling in the liver.
29365334	1	76	theme	several	143:149	arg1	chinensis					182:190	chinensis	182:190	chinensis	182:190	Berberine, a natural compound extracted from several Chinese herbs including Coptis chinensis, has been shown to have anti-obesity effects and prevents insulin resistance in high-fat diet (HFD)-fed obese rats by modulating the gut microbiota; however, the molecular mechanisms underlying these activities remain unknown.
29365334	1	76	theme	several	143:149	arg1	herbs					159:163	several Chinese herbs	143:163	several Chinese herbs including Coptis chinensis	143:190	Berberine, a natural compound extracted from several Chinese herbs including Coptis chinensis, has been shown to have anti-obesity effects and prevents insulin resistance in high-fat diet (HFD)-fed obese rats by modulating the gut microbiota; however, the molecular mechanisms underlying these activities remain unknown.
29365334	3	77	from	lipoprotein-cholesterol	779:801	arg1	rats					843:846	HFD-fed obese rats	829:846	HFD-fed obese rats	829:846	Our results showed that 8-week berberine (200 mg/kg) treatment significantly reduced fasting blood glucose, triglyceride, low-density lipoprotein-cholesterol and insulin resistance in HFD-fed obese rats.
31816881	3	0	dep	h.	688:689	arg1	revealed					776:783	revealed	776:783	revealed changes in the community structure of SIHUMIx during the change of TT, which were not associated with changes in species abundances	776:915	After five days of continuous cultivation, we investigated the influence of different medium TT of 12 h, 24 h, and 48 h. For profiling the microbial community, we applied flow cytometric fingerprinting and revealed changes in the community structure of SIHUMIx during the change of TT, which were not associated with changes in species abundances.
31816881	3	0	dep	h.	688:689	arg1	applied					733:739	applied	733:739	applied flow cytometric fingerprinting	733:770	After five days of continuous cultivation, we investigated the influence of different medium TT of 12 h, 24 h, and 48 h. For profiling the microbial community, we applied flow cytometric fingerprinting and revealed changes in the community structure of SIHUMIx during the change of TT, which were not associated with changes in species abundances.
31816881	3	1	theme	h.	688:689	arg1	TT					663:664	different medium TT	646:664	different medium TT of 12 h, 24 h, and 48 h. For profiling the microbial community, we applied flow cytometric fingerprinting and revealed changes in the community structure of SIHUMIx during the change of TT, which were not associated with changes in species abundances	646:915	After five days of continuous cultivation, we investigated the influence of different medium TT of 12 h, 24 h, and 48 h. For profiling the microbial community, we applied flow cytometric fingerprinting and revealed changes in the community structure of SIHUMIx during the change of TT, which were not associated with changes in species abundances.
31816881	0	2	theme	Structural	64:73	arg1	Resistance					90:99	High Structural and Functional Resistance	59:99	High Structural and Functional Resistance against Changing Transit Times in In Vitro Bioreactors	59:154	The Simplified Human Intestinal Microbiota (SIHUMIx) Shows High Structural and Functional Resistance against Changing Transit Times in In Vitro Bioreactors.
31816881	1	3	theme	Many	157:160	arg1	functions					162:170	Many functions	157:170	Many functions in host-microbiota interactions	157:202	Many functions in host-microbiota interactions are potentially influenced by intestinal transit times, but little is known about the effects of altered transition times on the composition and functionality of gut microbiota.
31816881	2	4	from	SIHUMIx	442:448	arg1	bioreactors					453:463	bioreactors	453:463	bioreactors	453:463	To analyze these effects, we cultivated the model community SIHUMIx in bioreactors in order to determine the effects of varying transit times (TT) on the community structure and function.
31816881	4	5	dep	found	1004:1008	arg1	shortening					1022:1031	shortening	1022:1031	shortening the TT	1022:1038	For pinpointing metabolic alterations, we applied metaproteomics and metabolomics and found, along with shortening the TT, a slight decrease in glycan biosynthesis, carbohydrate, and amino acid metabolism and, furthermore, a reduction in butyrate, methyl butyrate, isobutyrate, valerate, and isovalerate concentrations.
31816881	3	6	theme	SIHUMIx	823:829	arg1	structure					810:818	the community structure	796:818	the community structure of SIHUMIx	796:829	After five days of continuous cultivation, we investigated the influence of different medium TT of 12 h, 24 h, and 48 h. For profiling the microbial community, we applied flow cytometric fingerprinting and revealed changes in the community structure of SIHUMIx during the change of TT, which were not associated with changes in species abundances.
31816881	0	7	theme	Functional	79:88	arg1	Resistance					90:99	High Structural and Functional Resistance	59:99	High Structural and Functional Resistance against Changing Transit Times in In Vitro Bioreactors	59:154	The Simplified Human Intestinal Microbiota (SIHUMIx) Shows High Structural and Functional Resistance against Changing Transit Times in In Vitro Bioreactors.
31816881	4	8	theme	acid	1107:1110	arg1	metabolism					1112:1121	amino acid metabolism	1101:1121	amino acid metabolism	1101:1121	For pinpointing metabolic alterations, we applied metaproteomics and metabolomics and found, along with shortening the TT, a slight decrease in glycan biosynthesis, carbohydrate, and amino acid metabolism and, furthermore, a reduction in butyrate, methyl butyrate, isobutyrate, valerate, and isovalerate concentrations.
31816881	4	9	theme	methyl	1166:1171	arg1	butyrate					1173:1180	methyl butyrate	1166:1180	methyl butyrate	1166:1180	For pinpointing metabolic alterations, we applied metaproteomics and metabolomics and found, along with shortening the TT, a slight decrease in glycan biosynthesis, carbohydrate, and amino acid metabolism and, furthermore, a reduction in butyrate, methyl butyrate, isobutyrate, valerate, and isovalerate concentrations.
31816881	3	10	theme	TT	852:853	arg1	change					842:847	the change	838:847	the change of TT, which were not associated with changes in species abundances	838:915	After five days of continuous cultivation, we investigated the influence of different medium TT of 12 h, 24 h, and 48 h. For profiling the microbial community, we applied flow cytometric fingerprinting and revealed changes in the community structure of SIHUMIx during the change of TT, which were not associated with changes in species abundances.
31816881	8	11	theme	Resistance	1498:1507	arg1	values					1509:1514	Resistance values	1498:1514	Resistance values	1498:1514	Resistance values remained high, which suggests that TTs did not interfere with the structure of the community to a certain degree.
31816881	2	12	theme	transit	510:516	arg1	TT					525:526	TT	525:526	TT	525:526	To analyze these effects, we cultivated the model community SIHUMIx in bioreactors in order to determine the effects of varying transit times (TT) on the community structure and function.
31816881	2	12	theme	transit	510:516	arg1	times					518:522	varying transit times	502:522	varying transit times (TT)	502:527	To analyze these effects, we cultivated the model community SIHUMIx in bioreactors in order to determine the effects of varying transit times (TT) on the community structure and function.
31816881	1	13	theme	gut	366:368	arg1	microbiota					370:379	gut microbiota	366:379	gut microbiota	366:379	Many functions in host-microbiota interactions are potentially influenced by intestinal transit times, but little is known about the effects of altered transition times on the composition and functionality of gut microbiota.
31816881	1	14	from	effects	290:296	arg1	functionality					349:361	functionality	349:361	functionality	349:361	Many functions in host-microbiota interactions are potentially influenced by intestinal transit times, but little is known about the effects of altered transition times on the composition and functionality of gut microbiota.
31816881	1	14	from	effects	290:296	arg1	composition					333:343	composition	333:343	composition	333:343	Many functions in host-microbiota interactions are potentially influenced by intestinal transit times, but little is known about the effects of altered transition times on the composition and functionality of gut microbiota.
31816881	2	15	theme	varying	502:508	arg1	TT					525:526	TT	525:526	TT	525:526	To analyze these effects, we cultivated the model community SIHUMIx in bioreactors in order to determine the effects of varying transit times (TT) on the community structure and function.
31816881	2	15	theme	varying	502:508	arg1	times					518:522	varying transit times	502:522	varying transit times (TT)	502:527	To analyze these effects, we cultivated the model community SIHUMIx in bioreactors in order to determine the effects of varying transit times (TT) on the community structure and function.
31816881	1	16	theme	microbiota	370:379	arg1	functionality					349:361	functionality	349:361	functionality	349:361	Many functions in host-microbiota interactions are potentially influenced by intestinal transit times, but little is known about the effects of altered transition times on the composition and functionality of gut microbiota.
31816881	1	16	theme	microbiota	370:379	arg1	composition					333:343	composition	333:343	composition	333:343	Many functions in host-microbiota interactions are potentially influenced by intestinal transit times, but little is known about the effects of altered transition times on the composition and functionality of gut microbiota.
31816881	3	17	theme	cytometric	746:755	arg1	fingerprinting					757:770	flow cytometric fingerprinting	741:770	flow cytometric fingerprinting	741:770	After five days of continuous cultivation, we investigated the influence of different medium TT of 12 h, 24 h, and 48 h. For profiling the microbial community, we applied flow cytometric fingerprinting and revealed changes in the community structure of SIHUMIx during the change of TT, which were not associated with changes in species abundances.
31816881	0	18	theme	Simplified	4:13	arg1	SIHUMIx					44:50	SIHUMIx	44:50	SIHUMIx	44:50	The Simplified Human Intestinal Microbiota (SIHUMIx) Shows High Structural and Functional Resistance against Changing Transit Times in In Vitro Bioreactors.
31816881	0	18	theme	Simplified	4:13	arg1	Microbiota					32:41	The Simplified Human Intestinal Microbiota	0:41	The Simplified Human Intestinal Microbiota (SIHUMIx)	0:51	The Simplified Human Intestinal Microbiota (SIHUMIx) Shows High Structural and Functional Resistance against Changing Transit Times in In Vitro Bioreactors.
31816881	3	19	theme	h	678:678	arg1	TT					663:664	different medium TT	646:664	different medium TT of 12 h, 24 h, and 48 h. For profiling the microbial community, we applied flow cytometric fingerprinting and revealed changes in the community structure of SIHUMIx during the change of TT, which were not associated with changes in species abundances	646:915	After five days of continuous cultivation, we investigated the influence of different medium TT of 12 h, 24 h, and 48 h. For profiling the microbial community, we applied flow cytometric fingerprinting and revealed changes in the community structure of SIHUMIx during the change of TT, which were not associated with changes in species abundances.
31816881	4	20	from	reduction	1143:1151	arg1	butyrate					1173:1180	methyl butyrate	1166:1180	methyl butyrate	1166:1180	For pinpointing metabolic alterations, we applied metaproteomics and metabolomics and found, along with shortening the TT, a slight decrease in glycan biosynthesis, carbohydrate, and amino acid metabolism and, furthermore, a reduction in butyrate, methyl butyrate, isobutyrate, valerate, and isovalerate concentrations.
31816881	4	20	from	reduction	1143:1151	arg1	biosynthesis					1069:1080	glycan biosynthesis	1062:1080	glycan biosynthesis	1062:1080	For pinpointing metabolic alterations, we applied metaproteomics and metabolomics and found, along with shortening the TT, a slight decrease in glycan biosynthesis, carbohydrate, and amino acid metabolism and, furthermore, a reduction in butyrate, methyl butyrate, isobutyrate, valerate, and isovalerate concentrations.
31816881	4	20	from	reduction	1143:1151	arg1	isobutyrate					1183:1193	isobutyrate	1183:1193	isobutyrate	1183:1193	For pinpointing metabolic alterations, we applied metaproteomics and metabolomics and found, along with shortening the TT, a slight decrease in glycan biosynthesis, carbohydrate, and amino acid metabolism and, furthermore, a reduction in butyrate, methyl butyrate, isobutyrate, valerate, and isovalerate concentrations.
31816881	4	20	from	reduction	1143:1151	arg1	metabolism					1112:1121	amino acid metabolism	1101:1121	amino acid metabolism	1101:1121	For pinpointing metabolic alterations, we applied metaproteomics and metabolomics and found, along with shortening the TT, a slight decrease in glycan biosynthesis, carbohydrate, and amino acid metabolism and, furthermore, a reduction in butyrate, methyl butyrate, isobutyrate, valerate, and isovalerate concentrations.
31816881	4	20	from	reduction	1143:1151	arg1	concentrations					1222:1235	isovalerate concentrations	1210:1235	isovalerate concentrations	1210:1235	For pinpointing metabolic alterations, we applied metaproteomics and metabolomics and found, along with shortening the TT, a slight decrease in glycan biosynthesis, carbohydrate, and amino acid metabolism and, furthermore, a reduction in butyrate, methyl butyrate, isobutyrate, valerate, and isovalerate concentrations.
31816881	4	20	from	reduction	1143:1151	arg1	butyrate					1156:1163	butyrate	1156:1163	butyrate	1156:1163	For pinpointing metabolic alterations, we applied metaproteomics and metabolomics and found, along with shortening the TT, a slight decrease in glycan biosynthesis, carbohydrate, and amino acid metabolism and, furthermore, a reduction in butyrate, methyl butyrate, isobutyrate, valerate, and isovalerate concentrations.
31816881	4	20	from	reduction	1143:1151	arg1	carbohydrate					1083:1094	carbohydrate	1083:1094	carbohydrate	1083:1094	For pinpointing metabolic alterations, we applied metaproteomics and metabolomics and found, along with shortening the TT, a slight decrease in glycan biosynthesis, carbohydrate, and amino acid metabolism and, furthermore, a reduction in butyrate, methyl butyrate, isobutyrate, valerate, and isovalerate concentrations.
31816881	4	20	from	reduction	1143:1151	arg1	valerate					1196:1203	valerate	1196:1203	valerate	1196:1203	For pinpointing metabolic alterations, we applied metaproteomics and metabolomics and found, along with shortening the TT, a slight decrease in glycan biosynthesis, carbohydrate, and amino acid metabolism and, furthermore, a reduction in butyrate, methyl butyrate, isobutyrate, valerate, and isovalerate concentrations.
31816881	1	21	theme	host-microbiota	175:189	arg1	interactions					191:202	host-microbiota interactions	175:202	host-microbiota interactions	175:202	Many functions in host-microbiota interactions are potentially influenced by intestinal transit times, but little is known about the effects of altered transition times on the composition and functionality of gut microbiota.
31816881	7	22	theme	high	1440:1443	arg1	stability					1456:1464	high structural stability	1440:1464	high structural stability	1440:1464	This study shows that SIHUMIx showed high structural stability when TT changed-even four-fold.
31816881	0	23	theme	Intestinal	21:30	arg1	SIHUMIx					44:50	SIHUMIx	44:50	SIHUMIx	44:50	The Simplified Human Intestinal Microbiota (SIHUMIx) Shows High Structural and Functional Resistance against Changing Transit Times in In Vitro Bioreactors.
31816881	0	23	theme	Intestinal	21:30	arg1	Microbiota					32:41	The Simplified Human Intestinal Microbiota	0:41	The Simplified Human Intestinal Microbiota (SIHUMIx)	0:51	The Simplified Human Intestinal Microbiota (SIHUMIx) Shows High Structural and Functional Resistance against Changing Transit Times in In Vitro Bioreactors.
31816881	4	24	theme	glycan	1062:1067	arg1	biosynthesis					1069:1080	glycan biosynthesis	1062:1080	glycan biosynthesis	1062:1080	For pinpointing metabolic alterations, we applied metaproteomics and metabolomics and found, along with shortening the TT, a slight decrease in glycan biosynthesis, carbohydrate, and amino acid metabolism and, furthermore, a reduction in butyrate, methyl butyrate, isobutyrate, valerate, and isovalerate concentrations.
31816881	4	25	from	decrease	1050:1057	arg1	butyrate					1173:1180	methyl butyrate	1166:1180	methyl butyrate	1166:1180	For pinpointing metabolic alterations, we applied metaproteomics and metabolomics and found, along with shortening the TT, a slight decrease in glycan biosynthesis, carbohydrate, and amino acid metabolism and, furthermore, a reduction in butyrate, methyl butyrate, isobutyrate, valerate, and isovalerate concentrations.
31816881	4	25	from	decrease	1050:1057	arg1	biosynthesis					1069:1080	glycan biosynthesis	1062:1080	glycan biosynthesis	1062:1080	For pinpointing metabolic alterations, we applied metaproteomics and metabolomics and found, along with shortening the TT, a slight decrease in glycan biosynthesis, carbohydrate, and amino acid metabolism and, furthermore, a reduction in butyrate, methyl butyrate, isobutyrate, valerate, and isovalerate concentrations.
31816881	4	25	from	decrease	1050:1057	arg1	isobutyrate					1183:1193	isobutyrate	1183:1193	isobutyrate	1183:1193	For pinpointing metabolic alterations, we applied metaproteomics and metabolomics and found, along with shortening the TT, a slight decrease in glycan biosynthesis, carbohydrate, and amino acid metabolism and, furthermore, a reduction in butyrate, methyl butyrate, isobutyrate, valerate, and isovalerate concentrations.
31816881	4	25	from	decrease	1050:1057	arg1	metabolism					1112:1121	amino acid metabolism	1101:1121	amino acid metabolism	1101:1121	For pinpointing metabolic alterations, we applied metaproteomics and metabolomics and found, along with shortening the TT, a slight decrease in glycan biosynthesis, carbohydrate, and amino acid metabolism and, furthermore, a reduction in butyrate, methyl butyrate, isobutyrate, valerate, and isovalerate concentrations.
31816881	4	25	from	decrease	1050:1057	arg1	concentrations					1222:1235	isovalerate concentrations	1210:1235	isovalerate concentrations	1210:1235	For pinpointing metabolic alterations, we applied metaproteomics and metabolomics and found, along with shortening the TT, a slight decrease in glycan biosynthesis, carbohydrate, and amino acid metabolism and, furthermore, a reduction in butyrate, methyl butyrate, isobutyrate, valerate, and isovalerate concentrations.
31816881	4	25	from	decrease	1050:1057	arg1	butyrate					1156:1163	butyrate	1156:1163	butyrate	1156:1163	For pinpointing metabolic alterations, we applied metaproteomics and metabolomics and found, along with shortening the TT, a slight decrease in glycan biosynthesis, carbohydrate, and amino acid metabolism and, furthermore, a reduction in butyrate, methyl butyrate, isobutyrate, valerate, and isovalerate concentrations.
31816881	4	25	from	decrease	1050:1057	arg1	carbohydrate					1083:1094	carbohydrate	1083:1094	carbohydrate	1083:1094	For pinpointing metabolic alterations, we applied metaproteomics and metabolomics and found, along with shortening the TT, a slight decrease in glycan biosynthesis, carbohydrate, and amino acid metabolism and, furthermore, a reduction in butyrate, methyl butyrate, isobutyrate, valerate, and isovalerate concentrations.
31816881	4	25	from	decrease	1050:1057	arg1	valerate					1196:1203	valerate	1196:1203	valerate	1196:1203	For pinpointing metabolic alterations, we applied metaproteomics and metabolomics and found, along with shortening the TT, a slight decrease in glycan biosynthesis, carbohydrate, and amino acid metabolism and, furthermore, a reduction in butyrate, methyl butyrate, isobutyrate, valerate, and isovalerate concentrations.
31816881	0	26	theme	Human	15:19	arg1	SIHUMIx					44:50	SIHUMIx	44:50	SIHUMIx	44:50	The Simplified Human Intestinal Microbiota (SIHUMIx) Shows High Structural and Functional Resistance against Changing Transit Times in In Vitro Bioreactors.
31816881	0	26	theme	Human	15:19	arg1	Microbiota					32:41	The Simplified Human Intestinal Microbiota	0:41	The Simplified Human Intestinal Microbiota (SIHUMIx)	0:51	The Simplified Human Intestinal Microbiota (SIHUMIx) Shows High Structural and Functional Resistance against Changing Transit Times in In Vitro Bioreactors.
31816881	0	27	theme	Transit	118:124	arg1	Times					126:130	Transit Times	118:130	Transit Times	118:130	The Simplified Human Intestinal Microbiota (SIHUMIx) Shows High Structural and Functional Resistance against Changing Transit Times in In Vitro Bioreactors.
31816881	5	28	theme	B.	1252:1253	arg1	thetaiotaomicron					1255:1270	B. thetaiotaomicron	1252:1270	B. thetaiotaomicron	1252:1270	Specifically, B. thetaiotaomicron was identified to be affected in terms of butyrate metabolism.
31816881	3	29	from	changes	887:893	arg1	abundances					906:915	species abundances	898:915	species abundances	898:915	After five days of continuous cultivation, we investigated the influence of different medium TT of 12 h, 24 h, and 48 h. For profiling the microbial community, we applied flow cytometric fingerprinting and revealed changes in the community structure of SIHUMIx during the change of TT, which were not associated with changes in species abundances.
31816881	1	30	dep	composition	333:343	arg1	the					329:331	the	329:331	the	329:331	Many functions in host-microbiota interactions are potentially influenced by intestinal transit times, but little is known about the effects of altered transition times on the composition and functionality of gut microbiota.
31816881	0	31	theme	In	135:136	arg1	Bioreactors					144:154	In Vitro Bioreactors	135:154	In Vitro Bioreactors	135:154	The Simplified Human Intestinal Microbiota (SIHUMIx) Shows High Structural and Functional Resistance against Changing Transit Times in In Vitro Bioreactors.
31816881	4	32	theme	slight	1043:1048	arg1	decrease					1050:1057	a slight decrease	1041:1057	a slight decrease in glycan biosynthesis, carbohydrate, and amino acid metabolism	1041:1121	For pinpointing metabolic alterations, we applied metaproteomics and metabolomics and found, along with shortening the TT, a slight decrease in glycan biosynthesis, carbohydrate, and amino acid metabolism and, furthermore, a reduction in butyrate, methyl butyrate, isobutyrate, valerate, and isovalerate concentrations.
31816881	4	33	theme	metabolic	934:942	arg1	alterations					944:954	metabolic alterations	934:954	metabolic alterations	934:954	For pinpointing metabolic alterations, we applied metaproteomics and metabolomics and found, along with shortening the TT, a slight decrease in glycan biosynthesis, carbohydrate, and amino acid metabolism and, furthermore, a reduction in butyrate, methyl butyrate, isobutyrate, valerate, and isovalerate concentrations.
31816881	1	34	theme	altered	301:307	arg1	times					320:324	altered transition times	301:324	altered transition times	301:324	Many functions in host-microbiota interactions are potentially influenced by intestinal transit times, but little is known about the effects of altered transition times on the composition and functionality of gut microbiota.
31816881	3	35	from	changes	785:791	arg1	structure					810:818	the community structure	796:818	the community structure of SIHUMIx	796:829	After five days of continuous cultivation, we investigated the influence of different medium TT of 12 h, 24 h, and 48 h. For profiling the microbial community, we applied flow cytometric fingerprinting and revealed changes in the community structure of SIHUMIx during the change of TT, which were not associated with changes in species abundances.
31816881	4	36	theme	isovalerate	1210:1220	arg1	concentrations					1222:1235	isovalerate concentrations	1210:1235	isovalerate concentrations	1210:1235	For pinpointing metabolic alterations, we applied metaproteomics and metabolomics and found, along with shortening the TT, a slight decrease in glycan biosynthesis, carbohydrate, and amino acid metabolism and, furthermore, a reduction in butyrate, methyl butyrate, isobutyrate, valerate, and isovalerate concentrations.
31816881	2	37	theme	community	432:440	arg1	SIHUMIx					442:448	the model community SIHUMIx	422:448	the model community SIHUMIx in bioreactors	422:463	To analyze these effects, we cultivated the model community SIHUMIx in bioreactors in order to determine the effects of varying transit times (TT) on the community structure and function.
31816881	3	38	theme	microbial	709:717	arg1	community					719:727	the microbial community	705:727	the microbial community	705:727	After five days of continuous cultivation, we investigated the influence of different medium TT of 12 h, 24 h, and 48 h. For profiling the microbial community, we applied flow cytometric fingerprinting and revealed changes in the community structure of SIHUMIx during the change of TT, which were not associated with changes in species abundances.
31816881	1	39	theme	transition	309:318	arg1	times					320:324	altered transition times	301:324	altered transition times	301:324	Many functions in host-microbiota interactions are potentially influenced by intestinal transit times, but little is known about the effects of altered transition times on the composition and functionality of gut microbiota.
31816881	2	40	from	effects	491:497	arg1	structure					546:554	the community structure	532:554	the community structure	532:554	To analyze these effects, we cultivated the model community SIHUMIx in bioreactors in order to determine the effects of varying transit times (TT) on the community structure and function.
31816881	2	40	from	effects	491:497	arg1	function					560:567	function	560:567	function	560:567	To analyze these effects, we cultivated the model community SIHUMIx in bioreactors in order to determine the effects of varying transit times (TT) on the community structure and function.
31816881	3	41	theme	species	898:904	arg1	abundances					906:915	species abundances	898:915	species abundances	898:915	After five days of continuous cultivation, we investigated the influence of different medium TT of 12 h, 24 h, and 48 h. For profiling the microbial community, we applied flow cytometric fingerprinting and revealed changes in the community structure of SIHUMIx during the change of TT, which were not associated with changes in species abundances.
31816881	1	42	theme	times	320:324	arg1	effects					290:296	the effects	286:296	the effects of altered transition times on the composition and functionality of gut microbiota	286:379	Many functions in host-microbiota interactions are potentially influenced by intestinal transit times, but little is known about the effects of altered transition times on the composition and functionality of gut microbiota.
31816881	3	43	theme	medium	656:661	arg1	TT					663:664	different medium TT	646:664	different medium TT of 12 h, 24 h, and 48 h. For profiling the microbial community, we applied flow cytometric fingerprinting and revealed changes in the community structure of SIHUMIx during the change of TT, which were not associated with changes in species abundances	646:915	After five days of continuous cultivation, we investigated the influence of different medium TT of 12 h, 24 h, and 48 h. For profiling the microbial community, we applied flow cytometric fingerprinting and revealed changes in the community structure of SIHUMIx during the change of TT, which were not associated with changes in species abundances.
31816881	7	44	theme	structural	1445:1454	arg1	stability					1456:1464	high structural stability	1440:1464	high structural stability	1440:1464	This study shows that SIHUMIx showed high structural stability when TT changed-even four-fold.
31816881	5	45	theme	butyrate	1314:1321	arg1	metabolism					1323:1332	butyrate metabolism	1314:1332	butyrate metabolism	1314:1332	Specifically, B. thetaiotaomicron was identified to be affected in terms of butyrate metabolism.
31816881	6	46	theme	original	1377:1384	arg1	state					1386:1390	the original state	1373:1390	the original state	1373:1390	However, communities could recover to the original state afterward.
31816881	3	47	theme	community	800:808	arg1	structure					810:818	the community structure	796:818	the community structure of SIHUMIx	796:829	After five days of continuous cultivation, we investigated the influence of different medium TT of 12 h, 24 h, and 48 h. For profiling the microbial community, we applied flow cytometric fingerprinting and revealed changes in the community structure of SIHUMIx during the change of TT, which were not associated with changes in species abundances.
31816881	3	48	theme	continuous	589:598	arg1	cultivation					600:610	continuous cultivation	589:610	continuous cultivation	589:610	After five days of continuous cultivation, we investigated the influence of different medium TT of 12 h, 24 h, and 48 h. For profiling the microbial community, we applied flow cytometric fingerprinting and revealed changes in the community structure of SIHUMIx during the change of TT, which were not associated with changes in species abundances.
31816881	3	49	theme	TT	663:664	arg1	influence					633:641	the influence	629:641	the influence of different medium TT of 12 h, 24 h, and 48 h. For profiling the microbial community, we applied flow cytometric fingerprinting and revealed changes in the community structure of SIHUMIx during the change of TT, which were not associated with changes in species abundances	629:915	After five days of continuous cultivation, we investigated the influence of different medium TT of 12 h, 24 h, and 48 h. For profiling the microbial community, we applied flow cytometric fingerprinting and revealed changes in the community structure of SIHUMIx during the change of TT, which were not associated with changes in species abundances.
31816881	3	50	theme	cultivation	600:610	arg1	days					581:584	five days	576:584	five days of continuous cultivation	576:610	After five days of continuous cultivation, we investigated the influence of different medium TT of 12 h, 24 h, and 48 h. For profiling the microbial community, we applied flow cytometric fingerprinting and revealed changes in the community structure of SIHUMIx during the change of TT, which were not associated with changes in species abundances.
31816881	5	51	theme	metabolism	1323:1332	arg1	terms					1305:1309	terms	1305:1309	terms of butyrate metabolism	1305:1332	Specifically, B. thetaiotaomicron was identified to be affected in terms of butyrate metabolism.
31816881	4	52	theme	amino	1101:1105	arg1	metabolism					1112:1121	amino acid metabolism	1101:1121	amino acid metabolism	1101:1121	For pinpointing metabolic alterations, we applied metaproteomics and metabolomics and found, along with shortening the TT, a slight decrease in glycan biosynthesis, carbohydrate, and amino acid metabolism and, furthermore, a reduction in butyrate, methyl butyrate, isobutyrate, valerate, and isovalerate concentrations.
31816881	1	53	theme	intestinal	234:243	arg1	times					253:257	intestinal transit times	234:257	intestinal transit times	234:257	Many functions in host-microbiota interactions are potentially influenced by intestinal transit times, but little is known about the effects of altered transition times on the composition and functionality of gut microbiota.
31816881	2	54	theme	community	536:544	arg1	structure					546:554	the community structure	532:554	the community structure	532:554	To analyze these effects, we cultivated the model community SIHUMIx in bioreactors in order to determine the effects of varying transit times (TT) on the community structure and function.
31816881	8	55	theme	community	1599:1607	arg1	structure					1582:1590	the structure	1578:1590	the structure of the community to a certain degree	1578:1627	Resistance values remained high, which suggests that TTs did not interfere with the structure of the community to a certain degree.
31816881	1	56	theme	transit	245:251	arg1	times					253:257	intestinal transit times	234:257	intestinal transit times	234:257	Many functions in host-microbiota interactions are potentially influenced by intestinal transit times, but little is known about the effects of altered transition times on the composition and functionality of gut microbiota.
31816881	0	57	theme	High	59:62	arg1	Resistance					90:99	High Structural and Functional Resistance	59:99	High Structural and Functional Resistance against Changing Transit Times in In Vitro Bioreactors	59:154	The Simplified Human Intestinal Microbiota (SIHUMIx) Shows High Structural and Functional Resistance against Changing Transit Times in In Vitro Bioreactors.
31816881	3	58	theme	different	646:654	arg1	TT					663:664	different medium TT	646:664	different medium TT of 12 h, 24 h, and 48 h. For profiling the microbial community, we applied flow cytometric fingerprinting and revealed changes in the community structure of SIHUMIx during the change of TT, which were not associated with changes in species abundances	646:915	After five days of continuous cultivation, we investigated the influence of different medium TT of 12 h, 24 h, and 48 h. For profiling the microbial community, we applied flow cytometric fingerprinting and revealed changes in the community structure of SIHUMIx during the change of TT, which were not associated with changes in species abundances.
31816881	0	59	dep	In	135:136	arg1	Vitro					138:142	Vitro	138:142	Vitro	138:142	The Simplified Human Intestinal Microbiota (SIHUMIx) Shows High Structural and Functional Resistance against Changing Transit Times in In Vitro Bioreactors.
31816881	8	60	theme	certain	1614:1620	arg1	degree					1622:1627	a certain degree	1612:1627	a certain degree	1612:1627	Resistance values remained high, which suggests that TTs did not interfere with the structure of the community to a certain degree.
31816881	3	61	theme	h	672:672	arg1	TT					663:664	different medium TT	646:664	different medium TT of 12 h, 24 h, and 48 h. For profiling the microbial community, we applied flow cytometric fingerprinting and revealed changes in the community structure of SIHUMIx during the change of TT, which were not associated with changes in species abundances	646:915	After five days of continuous cultivation, we investigated the influence of different medium TT of 12 h, 24 h, and 48 h. For profiling the microbial community, we applied flow cytometric fingerprinting and revealed changes in the community structure of SIHUMIx during the change of TT, which were not associated with changes in species abundances.
31816881	2	62	theme	model	426:430	arg1	SIHUMIx					442:448	the model community SIHUMIx	422:448	the model community SIHUMIx in bioreactors	422:463	To analyze these effects, we cultivated the model community SIHUMIx in bioreactors in order to determine the effects of varying transit times (TT) on the community structure and function.
31816881	1	63	from	functions	162:170	arg1	interactions					191:202	host-microbiota interactions	175:202	host-microbiota interactions	175:202	Many functions in host-microbiota interactions are potentially influenced by intestinal transit times, but little is known about the effects of altered transition times on the composition and functionality of gut microbiota.
31816881	2	64	theme	times	518:522	arg1	effects					491:497	the effects	487:497	the effects of varying transit times (TT) on the community structure and function	487:567	To analyze these effects, we cultivated the model community SIHUMIx in bioreactors in order to determine the effects of varying transit times (TT) on the community structure and function.
29487346	0	0	theme	MAPK	95:98	arg1	signalling					100:109	MAPK signalling	95:109	MAPK signalling	95:109	Galectin-1 enhances TNFα-induced inflammatory responses in Sertoli cells through activation of MAPK signalling.
29487346	10	1	theme	Sertoli	1381:1387	arg1	cells					1389:1393	Sertoli cells	1381:1393	Sertoli cells	1381:1393	Taken together, our data show that Gal-1 modulates inflammatory responses in Sertoli cells by enhancing the pro-inflammatory activity of TNFα via stimulation of MAPK signalling.
29487346	10	2	theme	MAPK	1465:1468	arg1	signalling					1470:1479	MAPK signalling	1465:1479	MAPK signalling	1465:1479	Taken together, our data show that Gal-1 modulates inflammatory responses in Sertoli cells by enhancing the pro-inflammatory activity of TNFα via stimulation of MAPK signalling.
29487346	6	3	theme	lectin	702:707	arg1	binding					709:715	lectin binding	702:715	lectin binding	702:715	Analyses of lectin binding and expression of glucosaminyl- and sialyltransferases indicated that the glycan composition on the cell surface of Sertoli and peritubular cells becomes less favourable for Gal-1 binding under inflammatory conditions.
29487346	4	4	theme	germ	570:573	arg1	cells					575:579	germ cells	570:579	germ cells	570:579	In normal rat testis Gal-1 was mainly expressed in Sertoli cells and germ cells.
29487346	4	5	theme	rat	511:513	arg1	testis					515:520	normal rat testis	504:520	normal rat testis	504:520	In normal rat testis Gal-1 was mainly expressed in Sertoli cells and germ cells.
29487346	6	6	theme	cell	817:820	arg1	surface					822:828	the cell surface	813:828	the cell surface of Sertoli and peritubular cells	813:861	Analyses of lectin binding and expression of glucosaminyl- and sialyltransferases indicated that the glycan composition on the cell surface of Sertoli and peritubular cells becomes less favourable for Gal-1 binding under inflammatory conditions.
29487346	8	7	with	Pretreatment	1028:1039	arg1	Gal-1					1046:1050	Gal-1	1046:1050	Gal-1	1046:1050	Pretreatment with Gal-1 synergistically and specifically enhanced TNFα-induced expression of MCP1, IL-1α, IL-6 and TNFα in Sertoli cells.
29487346	4	8	theme	normal	504:509	arg1	testis					515:520	normal rat testis	504:520	normal rat testis	504:520	In normal rat testis Gal-1 was mainly expressed in Sertoli cells and germ cells.
29487346	7	9	theme	Gal-1	961:965	arg1	expression					967:976	Gal-1 expression	961:976	Gal-1 expression	961:976	In primary Sertoli cells Gal-1 expression was found to be upregulated after TNFα challenge.
29487346	9	10	theme	Sertoli	1190:1196	arg1	cells					1198:1202	Sertoli cells	1190:1202	Sertoli cells	1190:1202	Combined stimulation of Sertoli cells with Gal-1 and TNFα enhanced the phosphorylation of MAP kinases as compared to TNFα or Gal-1 alone.
29487346	0	11	theme	signalling	100:109	arg1	activation					81:90	activation	81:90	activation of MAPK signalling	81:109	Galectin-1 enhances TNFα-induced inflammatory responses in Sertoli cells through activation of MAPK signalling.
29487346	5	12	theme	inflamed	589:596	arg1	testis					598:603	the inflamed testis	585:603	the inflamed testis	585:603	In the inflamed testis, Gal-1 expression was significantly downregulated most likely due to germ cell loss.
29487346	3	13	from	infiltrates	361:371	arg1	interstitium					380:391	the interstitium	376:391	the interstitium	376:391	EAO is characterized by leukocytic infiltrates in the interstitium, damage of spermatogenesis and production of inflammatory mediators like TNFα and MCP1 causing infertility.
29487346	5	14	theme	Gal-1	606:610	arg1	expression					612:621	Gal-1 expression	606:621	Gal-1 expression	606:621	In the inflamed testis, Gal-1 expression was significantly downregulated most likely due to germ cell loss.
29487346	6	15	theme	glycan	791:796	arg1	composition					798:808	the glycan composition	787:808	the glycan composition on the cell surface of Sertoli and peritubular cells	787:861	Analyses of lectin binding and expression of glucosaminyl- and sialyltransferases indicated that the glycan composition on the cell surface of Sertoli and peritubular cells becomes less favourable for Gal-1 binding under inflammatory conditions.
29487346	10	16	theme	inflammatory	1355:1366	arg1	responses					1368:1376	inflammatory responses	1355:1376	inflammatory responses in Sertoli cells	1355:1393	Taken together, our data show that Gal-1 modulates inflammatory responses in Sertoli cells by enhancing the pro-inflammatory activity of TNFα via stimulation of MAPK signalling.
29487346	6	17	from	composition	798:808	arg1	surface					822:828	the cell surface	813:828	the cell surface of Sertoli and peritubular cells	813:861	Analyses of lectin binding and expression of glucosaminyl- and sialyltransferases indicated that the glycan composition on the cell surface of Sertoli and peritubular cells becomes less favourable for Gal-1 binding under inflammatory conditions.
29487346	10	18	theme	TNFα	1441:1444	arg1	activity					1429:1436	the pro-inflammatory activity	1408:1436	the pro-inflammatory activity of TNFα	1408:1444	Taken together, our data show that Gal-1 modulates inflammatory responses in Sertoli cells by enhancing the pro-inflammatory activity of TNFα via stimulation of MAPK signalling.
29487346	2	19	theme	Gal-1	292:296	arg1	role					284:287	the role	280:287	the role of Gal-1 in testicular inflammation	280:323	Using a model of rat experimental autoimmune orchitis (EAO), we investigated the role of Gal-1 in testicular inflammation.
29487346	6	20	theme	Gal-1	891:895	arg1	binding					897:903	Gal-1 binding	891:903	Gal-1 binding	891:903	Analyses of lectin binding and expression of glucosaminyl- and sialyltransferases indicated that the glycan composition on the cell surface of Sertoli and peritubular cells becomes less favourable for Gal-1 binding under inflammatory conditions.
29487346	3	21	theme	spermatogenesis	404:418	arg1	production					424:433	production	424:433	production of inflammatory mediators like TNFα and MCP1 causing infertility	424:498	EAO is characterized by leukocytic infiltrates in the interstitium, damage of spermatogenesis and production of inflammatory mediators like TNFα and MCP1 causing infertility.
29487346	3	21	theme	spermatogenesis	404:418	arg1	damage					394:399	damage	394:399	damage of spermatogenesis	394:418	EAO is characterized by leukocytic infiltrates in the interstitium, damage of spermatogenesis and production of inflammatory mediators like TNFα and MCP1 causing infertility.
29487346	3	21	theme	spermatogenesis	404:418	arg1	infiltrates					361:371	leukocytic infiltrates	350:371	leukocytic infiltrates in the interstitium	350:391	EAO is characterized by leukocytic infiltrates in the interstitium, damage of spermatogenesis and production of inflammatory mediators like TNFα and MCP1 causing infertility.
29487346	6	22	theme	inflammatory	911:922	arg1	conditions					924:933	inflammatory conditions	911:933	inflammatory conditions	911:933	Analyses of lectin binding and expression of glucosaminyl- and sialyltransferases indicated that the glycan composition on the cell surface of Sertoli and peritubular cells becomes less favourable for Gal-1 binding under inflammatory conditions.
29487346	0	23	theme	inflammatory	33:44	arg1	responses					46:54	TNFα-induced inflammatory responses	20:54	TNFα-induced inflammatory responses	20:54	Galectin-1 enhances TNFα-induced inflammatory responses in Sertoli cells through activation of MAPK signalling.
29487346	1	24	theme	immune	185:190	arg1	responses					192:200	immune responses	185:200	immune responses	185:200	Galectin-1 (Gal-1) is a pleiotropic lectin involved in the modulation of immune responses.
29487346	3	25	theme	leukocytic	350:359	arg1	production					424:433	production	424:433	production of inflammatory mediators like TNFα and MCP1 causing infertility	424:498	EAO is characterized by leukocytic infiltrates in the interstitium, damage of spermatogenesis and production of inflammatory mediators like TNFα and MCP1 causing infertility.
29487346	3	25	theme	leukocytic	350:359	arg1	damage					394:399	damage	394:399	damage of spermatogenesis	394:418	EAO is characterized by leukocytic infiltrates in the interstitium, damage of spermatogenesis and production of inflammatory mediators like TNFα and MCP1 causing infertility.
29487346	3	25	theme	leukocytic	350:359	arg1	infiltrates					361:371	leukocytic infiltrates	350:371	leukocytic infiltrates in the interstitium	350:391	EAO is characterized by leukocytic infiltrates in the interstitium, damage of spermatogenesis and production of inflammatory mediators like TNFα and MCP1 causing infertility.
29487346	8	26	theme	MCP1	1121:1124	arg1	expression					1107:1116	TNFα-induced expression	1094:1116	TNFα-induced expression of MCP1, IL-1α, IL-6 and TNFα in Sertoli cells	1094:1163	Pretreatment with Gal-1 synergistically and specifically enhanced TNFα-induced expression of MCP1, IL-1α, IL-6 and TNFα in Sertoli cells.
29487346	7	27	theme	primary	939:945	arg1	cells					955:959	primary Sertoli cells	939:959	primary Sertoli cells	939:959	In primary Sertoli cells Gal-1 expression was found to be upregulated after TNFα challenge.
29487346	9	28	theme	cells	1198:1202	arg1	stimulation					1175:1185	Combined stimulation	1166:1185	Combined stimulation of Sertoli cells with Gal-1 and TNFα	1166:1222	Combined stimulation of Sertoli cells with Gal-1 and TNFα enhanced the phosphorylation of MAP kinases as compared to TNFα or Gal-1 alone.
29487346	1	29	theme	responses	192:200	arg1	modulation					171:180	the modulation	167:180	the modulation of immune responses	167:200	Galectin-1 (Gal-1) is a pleiotropic lectin involved in the modulation of immune responses.
29487346	0	30	theme	TNFα-induced	20:31	arg1	responses					46:54	TNFα-induced inflammatory responses	20:54	TNFα-induced inflammatory responses	20:54	Galectin-1 enhances TNFα-induced inflammatory responses in Sertoli cells through activation of MAPK signalling.
29487346	7	31	theme	TNFα	1012:1015	arg1	challenge					1017:1025	TNFα challenge	1012:1025	TNFα challenge	1012:1025	In primary Sertoli cells Gal-1 expression was found to be upregulated after TNFα challenge.
29487346	7	32	located	found	982:986	arg2	expression					967:976	Gal-1 expression	961:976	Gal-1 expression	961:976	In primary Sertoli cells Gal-1 expression was found to be upregulated after TNFα challenge.
29487346	7	32	located	found	982:986	arg1	cells					955:959	primary Sertoli cells	939:959	primary Sertoli cells	939:959	In primary Sertoli cells Gal-1 expression was found to be upregulated after TNFα challenge.
29487346	4	33	theme	Sertoli	552:558	arg1	cells					560:564	Sertoli cells	552:564	Sertoli cells	552:564	In normal rat testis Gal-1 was mainly expressed in Sertoli cells and germ cells.
29487346	8	34	theme	IL-1α	1127:1131	arg1	expression					1107:1116	TNFα-induced expression	1094:1116	TNFα-induced expression of MCP1, IL-1α, IL-6 and TNFα in Sertoli cells	1094:1163	Pretreatment with Gal-1 synergistically and specifically enhanced TNFα-induced expression of MCP1, IL-1α, IL-6 and TNFα in Sertoli cells.
29487346	9	35	theme	MAP	1256:1258	arg1	kinases					1260:1266	MAP kinases	1256:1266	MAP kinases	1256:1266	Combined stimulation of Sertoli cells with Gal-1 and TNFα enhanced the phosphorylation of MAP kinases as compared to TNFα or Gal-1 alone.
29487346	2	36	theme	experimental	224:235	arg1	orchitis					248:255	rat experimental autoimmune orchitis	220:255	rat experimental autoimmune orchitis (EAO)	220:261	Using a model of rat experimental autoimmune orchitis (EAO), we investigated the role of Gal-1 in testicular inflammation.
29487346	2	36	theme	experimental	224:235	arg1	EAO					258:260	EAO	258:260	EAO	258:260	Using a model of rat experimental autoimmune orchitis (EAO), we investigated the role of Gal-1 in testicular inflammation.
29487346	3	37	theme	inflammatory	438:449	arg1	mediators					451:459	inflammatory mediators	438:459	inflammatory mediators like TNFα and MCP1 causing infertility	438:498	EAO is characterized by leukocytic infiltrates in the interstitium, damage of spermatogenesis and production of inflammatory mediators like TNFα and MCP1 causing infertility.
29487346	8	38	from	expression	1107:1116	arg1	cells					1159:1163	Sertoli cells	1151:1163	Sertoli cells	1151:1163	Pretreatment with Gal-1 synergistically and specifically enhanced TNFα-induced expression of MCP1, IL-1α, IL-6 and TNFα in Sertoli cells.
29487346	6	39	theme	cells	857:861	arg1	surface					822:828	the cell surface	813:828	the cell surface of Sertoli and peritubular cells	813:861	Analyses of lectin binding and expression of glucosaminyl- and sialyltransferases indicated that the glycan composition on the cell surface of Sertoli and peritubular cells becomes less favourable for Gal-1 binding under inflammatory conditions.
29487346	2	40	theme	rat	220:222	arg1	orchitis					248:255	rat experimental autoimmune orchitis	220:255	rat experimental autoimmune orchitis (EAO)	220:261	Using a model of rat experimental autoimmune orchitis (EAO), we investigated the role of Gal-1 in testicular inflammation.
29487346	2	40	theme	rat	220:222	arg1	EAO					258:260	EAO	258:260	EAO	258:260	Using a model of rat experimental autoimmune orchitis (EAO), we investigated the role of Gal-1 in testicular inflammation.
29487346	6	41	theme	sialyltransferases	753:770	arg1	expression					721:730	expression	721:730	expression of glucosaminyl- and sialyltransferases	721:770	Analyses of lectin binding and expression of glucosaminyl- and sialyltransferases indicated that the glycan composition on the cell surface of Sertoli and peritubular cells becomes less favourable for Gal-1 binding under inflammatory conditions.
29487346	6	41	theme	sialyltransferases	753:770	arg1	Analyses					690:697	Analyses	690:697	Analyses of lectin binding	690:715	Analyses of lectin binding and expression of glucosaminyl- and sialyltransferases indicated that the glycan composition on the cell surface of Sertoli and peritubular cells becomes less favourable for Gal-1 binding under inflammatory conditions.
29487346	3	42	theme	mediators	451:459	arg1	production					424:433	production	424:433	production of inflammatory mediators like TNFα and MCP1 causing infertility	424:498	EAO is characterized by leukocytic infiltrates in the interstitium, damage of spermatogenesis and production of inflammatory mediators like TNFα and MCP1 causing infertility.
29487346	3	42	theme	mediators	451:459	arg1	damage					394:399	damage	394:399	damage of spermatogenesis	394:418	EAO is characterized by leukocytic infiltrates in the interstitium, damage of spermatogenesis and production of inflammatory mediators like TNFα and MCP1 causing infertility.
29487346	3	42	theme	mediators	451:459	arg1	infiltrates					361:371	leukocytic infiltrates	350:371	leukocytic infiltrates in the interstitium	350:391	EAO is characterized by leukocytic infiltrates in the interstitium, damage of spermatogenesis and production of inflammatory mediators like TNFα and MCP1 causing infertility.
29487346	8	43	theme	IL-6	1134:1137	arg1	expression					1107:1116	TNFα-induced expression	1094:1116	TNFα-induced expression of MCP1, IL-1α, IL-6 and TNFα in Sertoli cells	1094:1163	Pretreatment with Gal-1 synergistically and specifically enhanced TNFα-induced expression of MCP1, IL-1α, IL-6 and TNFα in Sertoli cells.
29487346	6	44	theme	peritubular	845:855	arg1	cells					857:861	peritubular cells	845:861	peritubular cells	845:861	Analyses of lectin binding and expression of glucosaminyl- and sialyltransferases indicated that the glycan composition on the cell surface of Sertoli and peritubular cells becomes less favourable for Gal-1 binding under inflammatory conditions.
29487346	8	45	theme	TNFα	1143:1146	arg1	expression					1107:1116	TNFα-induced expression	1094:1116	TNFα-induced expression of MCP1, IL-1α, IL-6 and TNFα in Sertoli cells	1094:1163	Pretreatment with Gal-1 synergistically and specifically enhanced TNFα-induced expression of MCP1, IL-1α, IL-6 and TNFα in Sertoli cells.
29487346	0	46	theme	Sertoli	59:65	arg1	cells					67:71	Sertoli cells	59:71	Sertoli cells	59:71	Galectin-1 enhances TNFα-induced inflammatory responses in Sertoli cells through activation of MAPK signalling.
29487346	10	47	theme	signalling	1470:1479	arg1	stimulation					1450:1460	stimulation	1450:1460	stimulation of MAPK signalling	1450:1479	Taken together, our data show that Gal-1 modulates inflammatory responses in Sertoli cells by enhancing the pro-inflammatory activity of TNFα via stimulation of MAPK signalling.
29487346	7	48	theme	Sertoli	947:953	arg1	cells					955:959	primary Sertoli cells	939:959	primary Sertoli cells	939:959	In primary Sertoli cells Gal-1 expression was found to be upregulated after TNFα challenge.
29487346	6	49	theme	glucosaminyl-	735:747	arg1	expression					721:730	expression	721:730	expression of glucosaminyl- and sialyltransferases	721:770	Analyses of lectin binding and expression of glucosaminyl- and sialyltransferases indicated that the glycan composition on the cell surface of Sertoli and peritubular cells becomes less favourable for Gal-1 binding under inflammatory conditions.
29487346	6	49	theme	glucosaminyl-	735:747	arg1	Analyses					690:697	Analyses	690:697	Analyses of lectin binding	690:715	Analyses of lectin binding and expression of glucosaminyl- and sialyltransferases indicated that the glycan composition on the cell surface of Sertoli and peritubular cells becomes less favourable for Gal-1 binding under inflammatory conditions.
29487346	9	50	theme	kinases	1260:1266	arg1	phosphorylation					1237:1251	the phosphorylation	1233:1251	the phosphorylation of MAP kinases	1233:1266	Combined stimulation of Sertoli cells with Gal-1 and TNFα enhanced the phosphorylation of MAP kinases as compared to TNFα or Gal-1 alone.
29487346	10	51	theme	pro-inflammatory	1412:1427	arg1	activity					1429:1436	the pro-inflammatory activity	1408:1436	the pro-inflammatory activity of TNFα	1408:1444	Taken together, our data show that Gal-1 modulates inflammatory responses in Sertoli cells by enhancing the pro-inflammatory activity of TNFα via stimulation of MAPK signalling.
29487346	5	52	theme	germ	674:677	arg1	loss					684:687	germ cell loss	674:687	germ cell loss	674:687	In the inflamed testis, Gal-1 expression was significantly downregulated most likely due to germ cell loss.
29487346	6	53	theme	Sertoli	833:839	arg1	surface					822:828	the cell surface	813:828	the cell surface of Sertoli and peritubular cells	813:861	Analyses of lectin binding and expression of glucosaminyl- and sialyltransferases indicated that the glycan composition on the cell surface of Sertoli and peritubular cells becomes less favourable for Gal-1 binding under inflammatory conditions.
29487346	2	54	theme	testicular	301:310	arg1	inflammation					312:323	testicular inflammation	301:323	testicular inflammation	301:323	Using a model of rat experimental autoimmune orchitis (EAO), we investigated the role of Gal-1 in testicular inflammation.
29487346	5	55	theme	cell	679:682	arg1	loss					684:687	germ cell loss	674:687	germ cell loss	674:687	In the inflamed testis, Gal-1 expression was significantly downregulated most likely due to germ cell loss.
29487346	9	56	theme	Combined	1166:1173	arg1	stimulation					1175:1185	Combined stimulation	1166:1185	Combined stimulation of Sertoli cells with Gal-1 and TNFα	1166:1222	Combined stimulation of Sertoli cells with Gal-1 and TNFα enhanced the phosphorylation of MAP kinases as compared to TNFα or Gal-1 alone.
29487346	8	57	theme	Sertoli	1151:1157	arg1	cells					1159:1163	Sertoli cells	1151:1163	Sertoli cells	1151:1163	Pretreatment with Gal-1 synergistically and specifically enhanced TNFα-induced expression of MCP1, IL-1α, IL-6 and TNFα in Sertoli cells.
29487346	8	58	theme	TNFα-induced	1094:1105	arg1	expression					1107:1116	TNFα-induced expression	1094:1116	TNFα-induced expression of MCP1, IL-1α, IL-6 and TNFα in Sertoli cells	1094:1163	Pretreatment with Gal-1 synergistically and specifically enhanced TNFα-induced expression of MCP1, IL-1α, IL-6 and TNFα in Sertoli cells.
29487346	2	59	theme	orchitis	248:255	arg1	model					211:215	a model	209:215	a model of rat experimental autoimmune orchitis (EAO)	209:261	Using a model of rat experimental autoimmune orchitis (EAO), we investigated the role of Gal-1 in testicular inflammation.
29487346	10	60	from	responses	1368:1376	arg1	cells					1389:1393	Sertoli cells	1381:1393	Sertoli cells	1381:1393	Taken together, our data show that Gal-1 modulates inflammatory responses in Sertoli cells by enhancing the pro-inflammatory activity of TNFα via stimulation of MAPK signalling.
29487346	1	61	theme	pleiotropic	136:146	arg1	lectin					148:153	a pleiotropic lectin	134:153	a pleiotropic lectin involved in the modulation of immune responses	134:200	Galectin-1 (Gal-1) is a pleiotropic lectin involved in the modulation of immune responses.
29487346	1	61	theme	pleiotropic	136:146	arg1	Galectin-1					112:121	Galectin-1	112:121	Galectin-1 (Gal-1)	112:129	Galectin-1 (Gal-1) is a pleiotropic lectin involved in the modulation of immune responses.
29487346	2	62	theme	autoimmune	237:246	arg1	orchitis					248:255	rat experimental autoimmune orchitis	220:255	rat experimental autoimmune orchitis (EAO)	220:261	Using a model of rat experimental autoimmune orchitis (EAO), we investigated the role of Gal-1 in testicular inflammation.
29487346	2	62	theme	autoimmune	237:246	arg1	EAO					258:260	EAO	258:260	EAO	258:260	Using a model of rat experimental autoimmune orchitis (EAO), we investigated the role of Gal-1 in testicular inflammation.
29487346	6	63	theme	binding	709:715	arg1	expression					721:730	expression	721:730	expression of glucosaminyl- and sialyltransferases	721:770	Analyses of lectin binding and expression of glucosaminyl- and sialyltransferases indicated that the glycan composition on the cell surface of Sertoli and peritubular cells becomes less favourable for Gal-1 binding under inflammatory conditions.
29487346	6	63	theme	binding	709:715	arg1	Analyses					690:697	Analyses	690:697	Analyses of lectin binding	690:715	Analyses of lectin binding and expression of glucosaminyl- and sialyltransferases indicated that the glycan composition on the cell surface of Sertoli and peritubular cells becomes less favourable for Gal-1 binding under inflammatory conditions.
29487346	2	64	from	role	284:287	arg1	inflammation					312:323	testicular inflammation	301:323	testicular inflammation	301:323	Using a model of rat experimental autoimmune orchitis (EAO), we investigated the role of Gal-1 in testicular inflammation.
29487346	9	65	with	stimulation	1175:1185	arg1	Gal-1					1209:1213	Gal-1	1209:1213	Gal-1	1209:1213	Combined stimulation of Sertoli cells with Gal-1 and TNFα enhanced the phosphorylation of MAP kinases as compared to TNFα or Gal-1 alone.
29487346	9	65	with	stimulation	1175:1185	arg1	TNFα					1219:1222	TNFα	1219:1222	TNFα	1219:1222	Combined stimulation of Sertoli cells with Gal-1 and TNFα enhanced the phosphorylation of MAP kinases as compared to TNFα or Gal-1 alone.
31014754	10	0	theme	HBV	1513:1515	arg1	diagnosis					1521:1529	HBV HCC diagnosis	1513:1529	HBV HCC diagnosis	1513:1529	CONCLUSION Quantitation of fucosylation levels on Hp protein using Lectin ELISA may be useful glycobiomarker either alone or in combination (AFP + DCP + FucHp; AUC = 0.94) in HBV HCC diagnosis in clinical practice.
31014754	3	1	from	identification	423:436	arg1	Hp					482:483	Hp	482:483	Hp	482:483	The present study promises diagnostic and prognostic markers by the identification of site-specific glycoforms on Haptoglobin (Hp) using LC-MS/MS and lectin ELISA in liver diseased conditions in HBV infection.
31014754	3	1	from	identification	423:436	arg1	Haptoglobin					469:479	Haptoglobin	469:479	Haptoglobin (Hp) using LC-MS/MS and lectin ELISA in liver diseased conditions in HBV infection	469:562	The present study promises diagnostic and prognostic markers by the identification of site-specific glycoforms on Haptoglobin (Hp) using LC-MS/MS and lectin ELISA in liver diseased conditions in HBV infection.
31014754	5	2	theme	column	717:722	arg1	chromatography					724:737	affinity column chromatography	708:737	affinity column chromatography	708:737	Hp was purified using affinity column chromatography and, peptide sequence, N-glycosylation site, glycan composition and glycoforms were identified using mass spectrometry.
31014754	0	3	theme	liver	78:82	arg1	diseases					84:91	liver diseases	78:91	liver diseases in Hepatitis B virus infection	78:122	Analysis of serum Haptoglobin using glycoproteomics and lectin immunoassay in liver diseases in Hepatitis B virus infection.
31014754	9	4	theme	advanced	1264:1271	arg1	stages					1287:1292	advanced liver disease stages	1264:1292	advanced liver disease stages	1264:1292	A significant association was observed between the Fuc-Hp/Hp Elisa index and, advanced liver disease stages and controls using lectin Elisa (p < .001).
31014754	10	5	theme	levels	1378:1383	arg1	Quantitation					1349:1360	CONCLUSION Quantitation	1338:1360	CONCLUSION Quantitation of fucosylation levels on Hp protein using Lectin ELISA	1338:1416	CONCLUSION Quantitation of fucosylation levels on Hp protein using Lectin ELISA may be useful glycobiomarker either alone or in combination (AFP + DCP + FucHp; AUC = 0.94) in HBV HCC diagnosis in clinical practice.
31014754	10	6	theme	Lectin	1405:1410	arg1	ELISA					1412:1416	Lectin ELISA	1405:1416	Lectin ELISA	1405:1416	CONCLUSION Quantitation of fucosylation levels on Hp protein using Lectin ELISA may be useful glycobiomarker either alone or in combination (AFP + DCP + FucHp; AUC = 0.94) in HBV HCC diagnosis in clinical practice.
31014754	6	7	used	used	889:892	arg2	ELISA					879:883	Quantitative lectin ELISA	859:883	Quantitative lectin ELISA	859:883	Quantitative lectin ELISA was used to measure levels of fucosylation on Hp in liver diseases due to HBV.
31014754	6	8	theme	Quantitative	859:870	arg1	ELISA					879:883	Quantitative lectin ELISA	859:883	Quantitative lectin ELISA	859:883	Quantitative lectin ELISA was used to measure levels of fucosylation on Hp in liver diseases due to HBV.
31014754	0	9	theme	Hepatitis	96:104	arg1	virus					108:112	Hepatitis B virus	96:112	Hepatitis B virus infection	96:122	Analysis of serum Haptoglobin using glycoproteomics and lectin immunoassay in liver diseases in Hepatitis B virus infection.
31014754	2	10	contain	having	314:319	arg1	marker					307:312	a marker	305:312	a marker having both sensitivity and specificity	305:352	Its early detection is of paramount importance using a marker having both sensitivity and specificity.
31014754	2	10	contain	having	314:319	arg2	specificity					342:352	specificity	342:352	specificity	342:352	Its early detection is of paramount importance using a marker having both sensitivity and specificity.
31014754	2	10	contain	having	314:319	arg2	sensitivity					326:336	sensitivity	326:336	sensitivity	326:336	Its early detection is of paramount importance using a marker having both sensitivity and specificity.
31014754	10	11	theme	fucosylation	1365:1376	arg1	levels					1378:1383	fucosylation levels	1365:1383	fucosylation levels	1365:1383	CONCLUSION Quantitation of fucosylation levels on Hp protein using Lectin ELISA may be useful glycobiomarker either alone or in combination (AFP + DCP + FucHp; AUC = 0.94) in HBV HCC diagnosis in clinical practice.
31014754	3	12	theme	liver	521:525	arg1	conditions					536:545	liver diseased conditions	521:545	liver diseased conditions in HBV infection	521:562	The present study promises diagnostic and prognostic markers by the identification of site-specific glycoforms on Haptoglobin (Hp) using LC-MS/MS and lectin ELISA in liver diseased conditions in HBV infection.
31014754	1	13	theme	Asia-Pacific	228:239	arg1	countries					241:249	Asia-Pacific countries	228:249	Asia-Pacific countries	228:249	BACKGROUND Hepatocellular carcinoma (HCC) due to Hepatitis B viral (HBV) infection is a major cause in Asia-Pacific countries.
31014754	10	14	theme	CONCLUSION	1338:1347	arg1	Quantitation					1349:1360	CONCLUSION Quantitation	1338:1360	CONCLUSION Quantitation of fucosylation levels on Hp protein using Lectin ELISA	1338:1416	CONCLUSION Quantitation of fucosylation levels on Hp protein using Lectin ELISA may be useful glycobiomarker either alone or in combination (AFP + DCP + FucHp; AUC = 0.94) in HBV HCC diagnosis in clinical practice.
31014754	9	15	theme	disease	1279:1285	arg1	stages					1287:1292	advanced liver disease stages	1264:1292	advanced liver disease stages	1264:1292	A significant association was observed between the Fuc-Hp/Hp Elisa index and, advanced liver disease stages and controls using lectin Elisa (p < .001).
31014754	3	16	theme	diseased	527:534	arg1	conditions					536:545	liver diseased conditions	521:545	liver diseased conditions in HBV infection	521:562	The present study promises diagnostic and prognostic markers by the identification of site-specific glycoforms on Haptoglobin (Hp) using LC-MS/MS and lectin ELISA in liver diseased conditions in HBV infection.
31014754	2	17	theme	early	256:260	arg1	detection					262:270	Its early detection	252:270	Its early detection	252:270	Its early detection is of paramount importance using a marker having both sensitivity and specificity.
31014754	10	18	from	Quantitation	1349:1360	arg1	protein					1391:1397	Hp protein	1388:1397	Hp protein using Lectin ELISA	1388:1416	CONCLUSION Quantitation of fucosylation levels on Hp protein using Lectin ELISA may be useful glycobiomarker either alone or in combination (AFP + DCP + FucHp; AUC = 0.94) in HBV HCC diagnosis in clinical practice.
31014754	8	19	theme	diseased	1153:1160	arg1	stages					1162:1167	liver diseased stages	1147:1167	liver diseased stages	1147:1167	Fucosylated glycoforms were significantly increased at site Asn184, Asn207 and Asn211 in liver diseased stages versus controls.
31014754	0	20	theme	virus	108:112	arg1	infection					114:122	Hepatitis B virus infection	96:122	Hepatitis B virus infection	96:122	Analysis of serum Haptoglobin using glycoproteomics and lectin immunoassay in liver diseases in Hepatitis B virus infection.
31014754	5	21	theme	mass	840:843	arg1	spectrometry					845:856	mass spectrometry	840:856	mass spectrometry	840:856	Hp was purified using affinity column chromatography and, peptide sequence, N-glycosylation site, glycan composition and glycoforms were identified using mass spectrometry.
31014754	0	22	theme	B	106:106	arg1	virus					108:112	Hepatitis B virus	96:112	Hepatitis B virus infection	96:122	Analysis of serum Haptoglobin using glycoproteomics and lectin immunoassay in liver diseases in Hepatitis B virus infection.
31014754	9	23	theme	liver	1273:1277	arg1	stages					1287:1292	advanced liver disease stages	1264:1292	advanced liver disease stages	1264:1292	A significant association was observed between the Fuc-Hp/Hp Elisa index and, advanced liver disease stages and controls using lectin Elisa (p < .001).
31014754	3	24	from	Haptoglobin	469:479	arg1	identification					423:436	the identification	419:436	the identification of site-specific glycoforms on Haptoglobin (Hp) using LC-MS/MS and lectin ELISA in liver diseased conditions in HBV infection	419:562	The present study promises diagnostic and prognostic markers by the identification of site-specific glycoforms on Haptoglobin (Hp) using LC-MS/MS and lectin ELISA in liver diseased conditions in HBV infection.
31014754	7	25	theme	Hp	972:973	arg1	levels					975:980	RESULTS Hp levels	964:980	RESULTS Hp levels	964:980	RESULTS Hp levels were significantly lower in HCC when compared with Non-HCC cases (p < .05).
31014754	8	26	theme	Fucosylated	1058:1068	arg1	glycoforms					1070:1079	Fucosylated glycoforms	1058:1079	Fucosylated glycoforms	1058:1079	Fucosylated glycoforms were significantly increased at site Asn184, Asn207 and Asn211 in liver diseased stages versus controls.
31014754	5	27	theme	affinity	708:715	arg1	chromatography					724:737	affinity column chromatography	708:737	affinity column chromatography	708:737	Hp was purified using affinity column chromatography and, peptide sequence, N-glycosylation site, glycan composition and glycoforms were identified using mass spectrometry.
31014754	3	28	theme	present	359:365	arg1	study					367:371	The present study	355:371	The present study	355:371	The present study promises diagnostic and prognostic markers by the identification of site-specific glycoforms on Haptoglobin (Hp) using LC-MS/MS and lectin ELISA in liver diseased conditions in HBV infection.
31014754	5	29	theme	peptide	744:750	arg1	sequence					752:759	peptide sequence	744:759	peptide sequence	744:759	Hp was purified using affinity column chromatography and, peptide sequence, N-glycosylation site, glycan composition and glycoforms were identified using mass spectrometry.
31014754	3	30	theme	HBV	550:552	arg1	infection					554:562	HBV infection	550:562	HBV infection	550:562	The present study promises diagnostic and prognostic markers by the identification of site-specific glycoforms on Haptoglobin (Hp) using LC-MS/MS and lectin ELISA in liver diseased conditions in HBV infection.
31014754	1	31	theme	Hepatitis	174:182	arg1	infection					198:206	Hepatitis B viral (HBV) infection	174:206	Hepatitis B viral (HBV) infection	174:206	BACKGROUND Hepatocellular carcinoma (HCC) due to Hepatitis B viral (HBV) infection is a major cause in Asia-Pacific countries.
31014754	5	32	theme	glycan	784:789	arg1	composition					791:801	glycan composition	784:801	glycan composition	784:801	Hp was purified using affinity column chromatography and, peptide sequence, N-glycosylation site, glycan composition and glycoforms were identified using mass spectrometry.
31014754	6	33	theme	fucosylation	915:926	arg1	levels					905:910	levels	905:910	levels of fucosylation	905:926	Quantitative lectin ELISA was used to measure levels of fucosylation on Hp in liver diseases due to HBV.
31014754	10	34	theme	Hp	1388:1389	arg1	protein					1391:1397	Hp protein	1388:1397	Hp protein using Lectin ELISA	1388:1416	CONCLUSION Quantitation of fucosylation levels on Hp protein using Lectin ELISA may be useful glycobiomarker either alone or in combination (AFP + DCP + FucHp; AUC = 0.94) in HBV HCC diagnosis in clinical practice.
31014754	2	35	theme	paramount	278:286	arg1	importance					288:297	paramount importance	278:297	paramount importance	278:297	Its early detection is of paramount importance using a marker having both sensitivity and specificity.
31014754	1	36	theme	B	184:184	arg1	infection					198:206	Hepatitis B viral (HBV) infection	174:206	Hepatitis B viral (HBV) infection	174:206	BACKGROUND Hepatocellular carcinoma (HCC) due to Hepatitis B viral (HBV) infection is a major cause in Asia-Pacific countries.
31014754	0	37	theme	Haptoglobin	18:28	arg1	Analysis					0:7	Analysis	0:7	Analysis of serum Haptoglobin	0:28	Analysis of serum Haptoglobin using glycoproteomics and lectin immunoassay in liver diseases in Hepatitis B virus infection.
31014754	6	38	theme	due	952:954	arg1	diseases					943:950	liver diseases	937:950	liver diseases due to HBV	937:961	Quantitative lectin ELISA was used to measure levels of fucosylation on Hp in liver diseases due to HBV.
31014754	9	39	theme	significant	1188:1198	arg1	association					1200:1210	A significant association	1186:1210	A significant association	1186:1210	A significant association was observed between the Fuc-Hp/Hp Elisa index and, advanced liver disease stages and controls using lectin Elisa (p < .001).
31014754	9	40	dep	index	1253:1257	arg1	the					1233:1235	the	1233:1235	the	1233:1235	A significant association was observed between the Fuc-Hp/Hp Elisa index and, advanced liver disease stages and controls using lectin Elisa (p < .001).
31014754	10	41	theme	useful	1425:1430	arg1	glycobiomarker					1432:1445	useful glycobiomarker	1425:1445	useful glycobiomarker either alone or in combination (AFP + DCP + FucHp; AUC = 0.94) in HBV HCC diagnosis in clinical practice	1425:1550	CONCLUSION Quantitation of fucosylation levels on Hp protein using Lectin ELISA may be useful glycobiomarker either alone or in combination (AFP + DCP + FucHp; AUC = 0.94) in HBV HCC diagnosis in clinical practice.
31014754	1	42	theme	viral	186:190	arg1	infection					198:206	Hepatitis B viral (HBV) infection	174:206	Hepatitis B viral (HBV) infection	174:206	BACKGROUND Hepatocellular carcinoma (HCC) due to Hepatitis B viral (HBV) infection is a major cause in Asia-Pacific countries.
31014754	0	43	theme	serum	12:16	arg1	Haptoglobin					18:28	serum Haptoglobin	12:28	serum Haptoglobin	12:28	Analysis of serum Haptoglobin using glycoproteomics and lectin immunoassay in liver diseases in Hepatitis B virus infection.
31014754	0	44	from	diseases	84:91	arg1	infection					114:122	Hepatitis B virus infection	96:122	Hepatitis B virus infection	96:122	Analysis of serum Haptoglobin using glycoproteomics and lectin immunoassay in liver diseases in Hepatitis B virus infection.
31014754	10	45	theme	HCC	1517:1519	arg1	diagnosis					1521:1529	HBV HCC diagnosis	1513:1529	HBV HCC diagnosis	1513:1529	CONCLUSION Quantitation of fucosylation levels on Hp protein using Lectin ELISA may be useful glycobiomarker either alone or in combination (AFP + DCP + FucHp; AUC = 0.94) in HBV HCC diagnosis in clinical practice.
31014754	0	46	theme	glycoproteomics	36:50	arg1	immunoassay					63:73	glycoproteomics and lectin immunoassay	36:73	immunoassay	63:73	Analysis of serum Haptoglobin using glycoproteomics and lectin immunoassay in liver diseases in Hepatitis B virus infection.
31014754	6	47	theme	liver	937:941	arg1	diseases					943:950	liver diseases	937:950	liver diseases due to HBV	937:961	Quantitative lectin ELISA was used to measure levels of fucosylation on Hp in liver diseases due to HBV.
31014754	1	48	from	cause	219:223	arg1	countries					241:249	Asia-Pacific countries	228:249	Asia-Pacific countries	228:249	BACKGROUND Hepatocellular carcinoma (HCC) due to Hepatitis B viral (HBV) infection is a major cause in Asia-Pacific countries.
31014754	10	49	dep	glycobiomarker	1432:1445	arg1	combination					1466:1476	combination	1466:1476	combination	1466:1476	CONCLUSION Quantitation of fucosylation levels on Hp protein using Lectin ELISA may be useful glycobiomarker either alone or in combination (AFP + DCP + FucHp; AUC = 0.94) in HBV HCC diagnosis in clinical practice.
31014754	10	49	dep	glycobiomarker	1432:1445	arg1	either					1447:1452	either	1447:1452	either	1447:1452	CONCLUSION Quantitation of fucosylation levels on Hp protein using Lectin ELISA may be useful glycobiomarker either alone or in combination (AFP + DCP + FucHp; AUC = 0.94) in HBV HCC diagnosis in clinical practice.
31014754	10	49	dep	glycobiomarker	1432:1445	arg1	AFP + DCP + FucHp					1479:1495	AFP + DCP + FucHp	1479:1495	AFP + DCP + FucHp; AUC = 0.94	1479:1507	CONCLUSION Quantitation of fucosylation levels on Hp protein using Lectin ELISA may be useful glycobiomarker either alone or in combination (AFP + DCP + FucHp; AUC = 0.94) in HBV HCC diagnosis in clinical practice.
31014754	4	50	with	chronic	599:605	arg1	infection					641:649	HBV infection	637:649	HBV infection	637:649	METHODS Three groups of patients: chronic, liver cirrhosis and HCC with HBV infection along with controls were enrolled.
31014754	4	51	theme	HBV	637:639	arg1	infection					641:649	HBV infection	637:649	HBV infection	637:649	METHODS Three groups of patients: chronic, liver cirrhosis and HCC with HBV infection along with controls were enrolled.
31014754	1	52	theme	HBV	193:195	arg1	infection					198:206	Hepatitis B viral (HBV) infection	174:206	Hepatitis B viral (HBV) infection	174:206	BACKGROUND Hepatocellular carcinoma (HCC) due to Hepatitis B viral (HBV) infection is a major cause in Asia-Pacific countries.
31014754	3	53	theme	prognostic	397:406	arg1	markers					408:414	diagnostic and prognostic markers	382:414	diagnostic and prognostic markers	382:414	The present study promises diagnostic and prognostic markers by the identification of site-specific glycoforms on Haptoglobin (Hp) using LC-MS/MS and lectin ELISA in liver diseased conditions in HBV infection.
31014754	10	54	from	glycobiomarker	1432:1445	arg1	diagnosis					1521:1529	HBV HCC diagnosis	1513:1529	HBV HCC diagnosis	1513:1529	CONCLUSION Quantitation of fucosylation levels on Hp protein using Lectin ELISA may be useful glycobiomarker either alone or in combination (AFP + DCP + FucHp; AUC = 0.94) in HBV HCC diagnosis in clinical practice.
31014754	10	54	from	glycobiomarker	1432:1445	arg1	practice					1543:1550	clinical practice	1534:1550	clinical practice	1534:1550	CONCLUSION Quantitation of fucosylation levels on Hp protein using Lectin ELISA may be useful glycobiomarker either alone or in combination (AFP + DCP + FucHp; AUC = 0.94) in HBV HCC diagnosis in clinical practice.
31014754	7	55	theme	Non-HCC	1033:1039	arg1	p < .05					1048:1054	p < .05	1048:1054	p < .05	1048:1054	RESULTS Hp levels were significantly lower in HCC when compared with Non-HCC cases (p < .05).
31014754	7	55	theme	Non-HCC	1033:1039	arg1	cases					1041:1045	Non-HCC cases	1033:1045	Non-HCC cases (p < .05)	1033:1055	RESULTS Hp levels were significantly lower in HCC when compared with Non-HCC cases (p < .05).
31014754	4	56	dep	METHODS	565:571	arg1	patients					589:596	patients	589:596	patients	589:596	METHODS Three groups of patients: chronic, liver cirrhosis and HCC with HBV infection along with controls were enrolled.
31014754	4	56	dep	METHODS	565:571	arg1	groups					579:584	Three groups	573:584	METHODS Three groups of patients: chronic, liver cirrhosis and HCC with HBV infection along with controls were enrolled.	565:684	METHODS Three groups of patients: chronic, liver cirrhosis and HCC with HBV infection along with controls were enrolled.
31014754	4	56	dep	METHODS	565:571	arg1	enrolled					676:683	enrolled	676:683	were enrolled	671:683	METHODS Three groups of patients: chronic, liver cirrhosis and HCC with HBV infection along with controls were enrolled.
31014754	3	57	gly	glycoforms	455:464	arg2	Haptoglobin					469:479	Haptoglobin	469:479	Haptoglobin (Hp) using LC-MS/MS and lectin ELISA in liver diseased conditions in HBV infection	469:562	The present study promises diagnostic and prognostic markers by the identification of site-specific glycoforms on Haptoglobin (Hp) using LC-MS/MS and lectin ELISA in liver diseased conditions in HBV infection.
31014754	3	57	gly	glycoforms	455:464	arg2	Hp					482:483	Hp	482:483	Hp	482:483	The present study promises diagnostic and prognostic markers by the identification of site-specific glycoforms on Haptoglobin (Hp) using LC-MS/MS and lectin ELISA in liver diseased conditions in HBV infection.
31014754	3	58	theme	site-specific	441:453	arg1	glycoforms					455:464	site-specific glycoforms	441:464	site-specific glycoforms on Haptoglobin (Hp) using LC-MS/MS and lectin ELISA in liver diseased conditions in HBV infection	441:562	The present study promises diagnostic and prognostic markers by the identification of site-specific glycoforms on Haptoglobin (Hp) using LC-MS/MS and lectin ELISA in liver diseased conditions in HBV infection.
31014754	0	59	theme	lectin	56:61	arg1	immunoassay					63:73	glycoproteomics and lectin immunoassay	36:73	immunoassay	63:73	Analysis of serum Haptoglobin using glycoproteomics and lectin immunoassay in liver diseases in Hepatitis B virus infection.
31014754	9	60	theme	lectin	1313:1318	arg1	Elisa					1320:1324	lectin Elisa	1313:1324	lectin Elisa (p < .001)	1313:1335	A significant association was observed between the Fuc-Hp/Hp Elisa index and, advanced liver disease stages and controls using lectin Elisa (p < .001).
31014754	9	60	theme	lectin	1313:1318	arg1	p < .001					1327:1334	p < .001	1327:1334	p < .001	1327:1334	A significant association was observed between the Fuc-Hp/Hp Elisa index and, advanced liver disease stages and controls using lectin Elisa (p < .001).
31014754	10	61	dep	AFP + DCP + FucHp	1479:1495	arg1	AUC = 0.94					1498:1507	AUC = 0.94	1498:1507	AUC = 0.94	1498:1507	CONCLUSION Quantitation of fucosylation levels on Hp protein using Lectin ELISA may be useful glycobiomarker either alone or in combination (AFP + DCP + FucHp; AUC = 0.94) in HBV HCC diagnosis in clinical practice.
31014754	3	62	theme	diagnostic	382:391	arg1	markers					408:414	diagnostic and prognostic markers	382:414	diagnostic and prognostic markers	382:414	The present study promises diagnostic and prognostic markers by the identification of site-specific glycoforms on Haptoglobin (Hp) using LC-MS/MS and lectin ELISA in liver diseased conditions in HBV infection.
31014754	10	63	dep	either	1447:1452	arg1	alone					1454:1458	alone	1454:1458	alone	1454:1458	CONCLUSION Quantitation of fucosylation levels on Hp protein using Lectin ELISA may be useful glycobiomarker either alone or in combination (AFP + DCP + FucHp; AUC = 0.94) in HBV HCC diagnosis in clinical practice.
31014754	3	64	from	glycoforms	455:464	arg1	Hp					482:483	Hp	482:483	Hp	482:483	The present study promises diagnostic and prognostic markers by the identification of site-specific glycoforms on Haptoglobin (Hp) using LC-MS/MS and lectin ELISA in liver diseased conditions in HBV infection.
31014754	3	64	from	glycoforms	455:464	arg1	Haptoglobin					469:479	Haptoglobin	469:479	Haptoglobin (Hp) using LC-MS/MS and lectin ELISA in liver diseased conditions in HBV infection	469:562	The present study promises diagnostic and prognostic markers by the identification of site-specific glycoforms on Haptoglobin (Hp) using LC-MS/MS and lectin ELISA in liver diseased conditions in HBV infection.
31014754	6	65	theme	lectin	872:877	arg1	ELISA					879:883	Quantitative lectin ELISA	859:883	Quantitative lectin ELISA	859:883	Quantitative lectin ELISA was used to measure levels of fucosylation on Hp in liver diseases due to HBV.
31014754	3	66	from	conditions	536:545	arg1	infection					554:562	HBV infection	550:562	HBV infection	550:562	The present study promises diagnostic and prognostic markers by the identification of site-specific glycoforms on Haptoglobin (Hp) using LC-MS/MS and lectin ELISA in liver diseased conditions in HBV infection.
31014754	5	67	gly	N-glycosylation	762:776	arg2	site					778:781	N-glycosylation site	762:781	N-glycosylation site	762:781	Hp was purified using affinity column chromatography and, peptide sequence, N-glycosylation site, glycan composition and glycoforms were identified using mass spectrometry.
31014754	5	68	dep	purified	693:700	arg1	identified					823:832	identified	823:832	were identified using mass spectrometry	818:856	Hp was purified using affinity column chromatography and, peptide sequence, N-glycosylation site, glycan composition and glycoforms were identified using mass spectrometry.
31014754	9	69	theme	Fuc-Hp/Hp	1237:1245	arg1	index					1253:1257	Fuc-Hp/Hp Elisa index	1237:1257	Fuc-Hp/Hp Elisa index	1237:1257	A significant association was observed between the Fuc-Hp/Hp Elisa index and, advanced liver disease stages and controls using lectin Elisa (p < .001).
31014754	1	70	theme	BACKGROUND	125:134	arg1	HCC					162:164	HCC	162:164	HCC	162:164	BACKGROUND Hepatocellular carcinoma (HCC) due to Hepatitis B viral (HBV) infection is a major cause in Asia-Pacific countries.
31014754	1	70	theme	BACKGROUND	125:134	arg1	cause					219:223	a major cause	211:223	a major cause in Asia-Pacific countries	211:249	BACKGROUND Hepatocellular carcinoma (HCC) due to Hepatitis B viral (HBV) infection is a major cause in Asia-Pacific countries.
31014754	1	70	theme	BACKGROUND	125:134	arg1	carcinoma					151:159	BACKGROUND Hepatocellular carcinoma	125:159	BACKGROUND Hepatocellular carcinoma (HCC) due to Hepatitis B viral (HBV) infection	125:206	BACKGROUND Hepatocellular carcinoma (HCC) due to Hepatitis B viral (HBV) infection is a major cause in Asia-Pacific countries.
31014754	4	71	theme	patients	589:596	arg1	patients					589:596	patients	589:596	patients	589:596	METHODS Three groups of patients: chronic, liver cirrhosis and HCC with HBV infection along with controls were enrolled.
31014754	4	71	theme	patients	589:596	arg1	groups					579:584	Three groups	573:584	METHODS Three groups of patients: chronic, liver cirrhosis and HCC with HBV infection along with controls were enrolled.	565:684	METHODS Three groups of patients: chronic, liver cirrhosis and HCC with HBV infection along with controls were enrolled.
31014754	6	72	from	Hp	931:932	arg1	diseases					943:950	liver diseases	937:950	liver diseases due to HBV	937:961	Quantitative lectin ELISA was used to measure levels of fucosylation on Hp in liver diseases due to HBV.
31014754	9	73	theme	Elisa	1247:1251	arg1	index					1253:1257	Fuc-Hp/Hp Elisa index	1237:1257	Fuc-Hp/Hp Elisa index	1237:1257	A significant association was observed between the Fuc-Hp/Hp Elisa index and, advanced liver disease stages and controls using lectin Elisa (p < .001).
31014754	8	74	theme	liver	1147:1151	arg1	stages					1162:1167	liver diseased stages	1147:1167	liver diseased stages	1147:1167	Fucosylated glycoforms were significantly increased at site Asn184, Asn207 and Asn211 in liver diseased stages versus controls.
31014754	7	75	theme	RESULTS	964:970	arg1	levels					975:980	RESULTS Hp levels	964:980	RESULTS Hp levels	964:980	RESULTS Hp levels were significantly lower in HCC when compared with Non-HCC cases (p < .05).
31014754	1	76	theme	Hepatocellular	136:149	arg1	HCC					162:164	HCC	162:164	HCC	162:164	BACKGROUND Hepatocellular carcinoma (HCC) due to Hepatitis B viral (HBV) infection is a major cause in Asia-Pacific countries.
31014754	1	76	theme	Hepatocellular	136:149	arg1	cause					219:223	a major cause	211:223	a major cause in Asia-Pacific countries	211:249	BACKGROUND Hepatocellular carcinoma (HCC) due to Hepatitis B viral (HBV) infection is a major cause in Asia-Pacific countries.
31014754	1	76	theme	Hepatocellular	136:149	arg1	carcinoma					151:159	BACKGROUND Hepatocellular carcinoma	125:159	BACKGROUND Hepatocellular carcinoma (HCC) due to Hepatitis B viral (HBV) infection	125:206	BACKGROUND Hepatocellular carcinoma (HCC) due to Hepatitis B viral (HBV) infection is a major cause in Asia-Pacific countries.
31014754	8	77	theme	site	1113:1116	arg1	Asn184					1118:1123	site Asn184	1113:1123	site Asn184	1113:1123	Fucosylated glycoforms were significantly increased at site Asn184, Asn207 and Asn211 in liver diseased stages versus controls.
31014754	3	78	theme	glycoforms	455:464	arg1	identification					423:436	the identification	419:436	the identification of site-specific glycoforms on Haptoglobin (Hp) using LC-MS/MS and lectin ELISA in liver diseased conditions in HBV infection	419:562	The present study promises diagnostic and prognostic markers by the identification of site-specific glycoforms on Haptoglobin (Hp) using LC-MS/MS and lectin ELISA in liver diseased conditions in HBV infection.
31014754	10	79	theme	clinical	1534:1541	arg1	practice					1543:1550	clinical practice	1534:1550	clinical practice	1534:1550	CONCLUSION Quantitation of fucosylation levels on Hp protein using Lectin ELISA may be useful glycobiomarker either alone or in combination (AFP + DCP + FucHp; AUC = 0.94) in HBV HCC diagnosis in clinical practice.
31014754	5	80	theme	N-glycosylation	762:776	arg1	site					778:781	N-glycosylation site	762:781	N-glycosylation site	762:781	Hp was purified using affinity column chromatography and, peptide sequence, N-glycosylation site, glycan composition and glycoforms were identified using mass spectrometry.
31014754	4	81	theme	liver	608:612	arg1	cirrhosis					614:622	liver cirrhosis	608:622	liver cirrhosis	608:622	METHODS Three groups of patients: chronic, liver cirrhosis and HCC with HBV infection along with controls were enrolled.
31014754	3	82	theme	lectin	505:510	arg1	ELISA					512:516	lectin ELISA	505:516	lectin ELISA	505:516	The present study promises diagnostic and prognostic markers by the identification of site-specific glycoforms on Haptoglobin (Hp) using LC-MS/MS and lectin ELISA in liver diseased conditions in HBV infection.
31014754	1	83	theme	major	213:217	arg1	carcinoma					151:159	BACKGROUND Hepatocellular carcinoma	125:159	BACKGROUND Hepatocellular carcinoma (HCC) due to Hepatitis B viral (HBV) infection	125:206	BACKGROUND Hepatocellular carcinoma (HCC) due to Hepatitis B viral (HBV) infection is a major cause in Asia-Pacific countries.
31014754	1	83	theme	major	213:217	arg1	cause					219:223	a major cause	211:223	a major cause in Asia-Pacific countries	211:249	BACKGROUND Hepatocellular carcinoma (HCC) due to Hepatitis B viral (HBV) infection is a major cause in Asia-Pacific countries.
30545140	0	0	theme	Sensory	78:84	arg1	Properties					86:95	the Chemical and Sensory Properties	61:95	the Chemical and Sensory Properties of Shiraz Wines	61:111	Impact of Commercial Oenotannin and Mannoprotein Products on the Chemical and Sensory Properties of Shiraz Wines Made from Sequentially Harvested Fruit.
30545140	1	1	theme	fruit	251:255	arg1	maturity					257:264	fruit maturity	251:264	fruit maturity at harvest, and practices employed during winemaking	251:317	The tannin and polysaccharide profiles and therefore sensory properties of wine are influenced by fruit maturity at harvest, and practices employed during winemaking.
30545140	2	2	theme	mouthfeel	461:469	arg1	properties					471:480	the mouthfeel properties	457:480	the mouthfeel properties of red wine	457:492	This study investigated the extent to which commercial winemaking supplements (skin and seed tannins, and mannoprotein (MP)) can enhance the mouthfeel properties of red wine, in particular, wine made from grapes harvested before commercial ripeness (early-harvest).
30545140	1	3	theme	tannin	157:162	arg1	profiles					183:190	The tannin and polysaccharide profiles	153:190	profiles	183:190	The tannin and polysaccharide profiles and therefore sensory properties of wine are influenced by fruit maturity at harvest, and practices employed during winemaking.
30545140	2	4	theme	wine	489:492	arg1	properties					471:480	the mouthfeel properties	457:480	the mouthfeel properties of red wine	457:492	This study investigated the extent to which commercial winemaking supplements (skin and seed tannins, and mannoprotein (MP)) can enhance the mouthfeel properties of red wine, in particular, wine made from grapes harvested before commercial ripeness (early-harvest).
30545140	7	5	dep	perceive	1224:1231	arg1	attributed					1346:1355	attributed	1346:1355	attributed	1346:1355	The DA panel perceived sensory differences between H1 and H2 wines, but could not perceive any effect of supplementation on wine mouthfeel properties, with the exception of a minor increase in sweetness, attributed to mannoprotein addition to H1 wines, even when MP was added to wines at 2.5 times the level recommended for use in Australia.
30545140	0	6	from	Impact	0:5	arg1	Properties					86:95	the Chemical and Sensory Properties	61:95	the Chemical and Sensory Properties of Shiraz Wines	61:111	Impact of Commercial Oenotannin and Mannoprotein Products on the Chemical and Sensory Properties of Shiraz Wines Made from Sequentially Harvested Fruit.
30545140	5	7	theme	riper	859:863	arg1	grapes					865:870	riper grapes	859:870	riper grapes	859:870	Wines made from riper grapes had higher levels of tannin than wines made from early-harvest grapes, but similar polysaccharide levels were observed.
30545140	5	8	theme	polysaccharide	955:968	arg1	levels					970:975	similar polysaccharide levels	947:975	similar polysaccharide levels	947:975	Wines made from riper grapes had higher levels of tannin than wines made from early-harvest grapes, but similar polysaccharide levels were observed.
30545140	4	9	dep	composition	689:699	arg1	The					676:678	The	676:678	The	676:678	The chemical composition and mouthfeel properties of wines were then determined by high performance liquid chromatography and descriptive analysis (DA), respectively.
30545140	4	10	theme	wines	729:733	arg1	properties					715:724	mouthfeel properties	705:724	mouthfeel properties	705:724	The chemical composition and mouthfeel properties of wines were then determined by high performance liquid chromatography and descriptive analysis (DA), respectively.
30545140	4	10	theme	wines	729:733	arg1	composition					689:699	chemical composition	680:699	chemical composition	680:699	The chemical composition and mouthfeel properties of wines were then determined by high performance liquid chromatography and descriptive analysis (DA), respectively.
30545140	7	11	from	exception	1302:1310	arg1	sweetness					1335:1343	sweetness	1335:1343	sweetness	1335:1343	The DA panel perceived sensory differences between H1 and H2 wines, but could not perceive any effect of supplementation on wine mouthfeel properties, with the exception of a minor increase in sweetness, attributed to mannoprotein addition to H1 wines, even when MP was added to wines at 2.5 times the level recommended for use in Australia.
30545140	5	12	theme	tannin	893:898	arg1	levels					883:888	higher levels	876:888	higher levels of tannin	876:898	Wines made from riper grapes had higher levels of tannin than wines made from early-harvest grapes, but similar polysaccharide levels were observed.
30545140	0	13	theme	Wines	107:111	arg1	Properties					86:95	the Chemical and Sensory Properties	61:95	the Chemical and Sensory Properties of Shiraz Wines	61:111	Impact of Commercial Oenotannin and Mannoprotein Products on the Chemical and Sensory Properties of Shiraz Wines Made from Sequentially Harvested Fruit.
30545140	1	14	from	practices	282:290	arg1	maturity					257:264	fruit maturity	251:264	fruit maturity at harvest, and practices employed during winemaking	251:317	The tannin and polysaccharide profiles and therefore sensory properties of wine are influenced by fruit maturity at harvest, and practices employed during winemaking.
30545140	6	15	theme	oenotannin	1013:1022	arg1	addition					996:1003	The addition	992:1003	The addition of seed oenotannin	992:1022	The addition of seed oenotannin yielded higher tannin levels than addition of skin oenotannin, particularly for wines made from early-harvest grapes.
30545140	0	16	theme	Shiraz	100:105	arg1	Wines					107:111	Shiraz Wines	100:111	Shiraz Wines	100:111	Impact of Commercial Oenotannin and Mannoprotein Products on the Chemical and Sensory Properties of Shiraz Wines Made from Sequentially Harvested Fruit.
30545140	7	17	from	sweetness	1335:1343	arg1	exception					1302:1310	the exception	1298:1310	the exception of a minor increase in sweetness	1298:1343	The DA panel perceived sensory differences between H1 and H2 wines, but could not perceive any effect of supplementation on wine mouthfeel properties, with the exception of a minor increase in sweetness, attributed to mannoprotein addition to H1 wines, even when MP was added to wines at 2.5 times the level recommended for use in Australia.
30545140	7	18	theme	mouthfeel	1271:1279	arg1	properties					1281:1290	wine mouthfeel properties	1266:1290	wine mouthfeel properties	1266:1290	The DA panel perceived sensory differences between H1 and H2 wines, but could not perceive any effect of supplementation on wine mouthfeel properties, with the exception of a minor increase in sweetness, attributed to mannoprotein addition to H1 wines, even when MP was added to wines at 2.5 times the level recommended for use in Australia.
30545140	7	19	theme	H1	1385:1386	arg1	wines					1388:1392	H1 wines	1385:1392	H1 wines	1385:1392	The DA panel perceived sensory differences between H1 and H2 wines, but could not perceive any effect of supplementation on wine mouthfeel properties, with the exception of a minor increase in sweetness, attributed to mannoprotein addition to H1 wines, even when MP was added to wines at 2.5 times the level recommended for use in Australia.
30545140	1	20	theme	polysaccharide	168:181	arg1	profiles					183:190	The tannin and polysaccharide profiles	153:190	profiles	183:190	The tannin and polysaccharide profiles and therefore sensory properties of wine are influenced by fruit maturity at harvest, and practices employed during winemaking.
30545140	7	21	theme	DA	1146:1147	arg1	panel					1149:1153	The DA panel	1142:1153	The DA panel	1142:1153	The DA panel perceived sensory differences between H1 and H2 wines, but could not perceive any effect of supplementation on wine mouthfeel properties, with the exception of a minor increase in sweetness, attributed to mannoprotein addition to H1 wines, even when MP was added to wines at 2.5 times the level recommended for use in Australia.
30545140	2	22	theme	red	485:487	arg1	wine					489:492	red wine	485:492	red wine	485:492	This study investigated the extent to which commercial winemaking supplements (skin and seed tannins, and mannoprotein (MP)) can enhance the mouthfeel properties of red wine, in particular, wine made from grapes harvested before commercial ripeness (early-harvest).
30545140	4	23	theme	mouthfeel	705:713	arg1	properties					715:724	mouthfeel properties	705:724	mouthfeel properties	705:724	The chemical composition and mouthfeel properties of wines were then determined by high performance liquid chromatography and descriptive analysis (DA), respectively.
30545140	6	24	theme	tannin	1039:1044	arg1	levels					1046:1051	higher tannin levels	1032:1051	higher tannin levels	1032:1051	The addition of seed oenotannin yielded higher tannin levels than addition of skin oenotannin, particularly for wines made from early-harvest grapes.
30545140	7	25	from	effect	1237:1242	arg1	properties					1281:1290	wine mouthfeel properties	1266:1290	wine mouthfeel properties	1266:1290	The DA panel perceived sensory differences between H1 and H2 wines, but could not perceive any effect of supplementation on wine mouthfeel properties, with the exception of a minor increase in sweetness, attributed to mannoprotein addition to H1 wines, even when MP was added to wines at 2.5 times the level recommended for use in Australia.
30545140	6	26	theme	early-harvest	1120:1132	arg1	grapes					1134:1139	early-harvest grapes	1120:1139	early-harvest grapes	1120:1139	The addition of seed oenotannin yielded higher tannin levels than addition of skin oenotannin, particularly for wines made from early-harvest grapes.
30545140	7	27	theme	minor	1317:1321	arg1	increase					1323:1330	a minor increase	1315:1330	a minor increase in sweetness	1315:1343	The DA panel perceived sensory differences between H1 and H2 wines, but could not perceive any effect of supplementation on wine mouthfeel properties, with the exception of a minor increase in sweetness, attributed to mannoprotein addition to H1 wines, even when MP was added to wines at 2.5 times the level recommended for use in Australia.
30545140	6	28	theme	higher	1032:1037	arg1	levels					1046:1051	higher tannin levels	1032:1051	higher tannin levels	1032:1051	The addition of seed oenotannin yielded higher tannin levels than addition of skin oenotannin, particularly for wines made from early-harvest grapes.
30545140	0	29	theme	Oenotannin	21:30	arg1	Impact					0:5	Impact	0:5	Impact of Commercial Oenotannin and Mannoprotein Products on the Chemical and Sensory Properties of Shiraz Wines	0:111	Impact of Commercial Oenotannin and Mannoprotein Products on the Chemical and Sensory Properties of Shiraz Wines Made from Sequentially Harvested Fruit.
30545140	7	30	from	increase	1323:1330	arg1	sweetness					1335:1343	sweetness	1335:1343	sweetness	1335:1343	The DA panel perceived sensory differences between H1 and H2 wines, but could not perceive any effect of supplementation on wine mouthfeel properties, with the exception of a minor increase in sweetness, attributed to mannoprotein addition to H1 wines, even when MP was added to wines at 2.5 times the level recommended for use in Australia.
30545140	5	31	theme	early-harvest	921:933	arg1	grapes					935:940	early-harvest grapes	921:940	early-harvest grapes	921:940	Wines made from riper grapes had higher levels of tannin than wines made from early-harvest grapes, but similar polysaccharide levels were observed.
30545140	0	32	theme	Commercial	10:19	arg1	Oenotannin					21:30	Commercial Oenotannin and Mannoprotein Products	10:56	Oenotannin	21:30	Impact of Commercial Oenotannin and Mannoprotein Products on the Chemical and Sensory Properties of Shiraz Wines Made from Sequentially Harvested Fruit.
30545140	1	33	from	harvest	269:275	arg1	maturity					257:264	fruit maturity	251:264	fruit maturity at harvest, and practices employed during winemaking	251:317	The tannin and polysaccharide profiles and therefore sensory properties of wine are influenced by fruit maturity at harvest, and practices employed during winemaking.
30545140	7	34	theme	mannoprotein	1360:1371	arg1	addition					1373:1380	mannoprotein addition	1360:1380	mannoprotein addition to H1 wines	1360:1392	The DA panel perceived sensory differences between H1 and H2 wines, but could not perceive any effect of supplementation on wine mouthfeel properties, with the exception of a minor increase in sweetness, attributed to mannoprotein addition to H1 wines, even when MP was added to wines at 2.5 times the level recommended for use in Australia.
30545140	1	35	theme	sensory	206:212	arg1	properties					214:223	therefore sensory properties	196:223	therefore sensory properties of wine	196:231	The tannin and polysaccharide profiles and therefore sensory properties of wine are influenced by fruit maturity at harvest, and practices employed during winemaking.
30545140	0	36	theme	Mannoprotein	36:47	arg1	Products					49:56	Commercial Oenotannin and Mannoprotein Products	10:56	Products	49:56	Impact of Commercial Oenotannin and Mannoprotein Products on the Chemical and Sensory Properties of Shiraz Wines Made from Sequentially Harvested Fruit.
30545140	0	37	theme	Harvested	136:144	arg1	Fruit					146:150	Sequentially Harvested Fruit	123:150	Sequentially Harvested Fruit	123:150	Impact of Commercial Oenotannin and Mannoprotein Products on the Chemical and Sensory Properties of Shiraz Wines Made from Sequentially Harvested Fruit.
30545140	7	38	theme	supplementation	1247:1261	arg1	effect					1237:1242	any effect	1233:1242	any effect of supplementation on wine mouthfeel properties	1233:1290	The DA panel perceived sensory differences between H1 and H2 wines, but could not perceive any effect of supplementation on wine mouthfeel properties, with the exception of a minor increase in sweetness, attributed to mannoprotein addition to H1 wines, even when MP was added to wines at 2.5 times the level recommended for use in Australia.
30545140	6	39	theme	seed	1008:1011	arg1	oenotannin					1013:1022	seed oenotannin	1008:1022	seed oenotannin	1008:1022	The addition of seed oenotannin yielded higher tannin levels than addition of skin oenotannin, particularly for wines made from early-harvest grapes.
30545140	7	40	dep	times	1434:1438	arg1	level					1444:1448	the level	1440:1448	2.5 times the level recommended for use in Australia	1430:1481	The DA panel perceived sensory differences between H1 and H2 wines, but could not perceive any effect of supplementation on wine mouthfeel properties, with the exception of a minor increase in sweetness, attributed to mannoprotein addition to H1 wines, even when MP was added to wines at 2.5 times the level recommended for use in Australia.
30545140	7	41	theme	sensory	1165:1171	arg1	differences					1173:1183	sensory differences	1165:1183	sensory differences between H1 and H2 wines	1165:1207	The DA panel perceived sensory differences between H1 and H2 wines, but could not perceive any effect of supplementation on wine mouthfeel properties, with the exception of a minor increase in sweetness, attributed to mannoprotein addition to H1 wines, even when MP was added to wines at 2.5 times the level recommended for use in Australia.
30545140	2	42	theme	seed	408:411	arg1	tannins					413:419	seed tannins	408:419	seed tannins	408:419	This study investigated the extent to which commercial winemaking supplements (skin and seed tannins, and mannoprotein (MP)) can enhance the mouthfeel properties of red wine, in particular, wine made from grapes harvested before commercial ripeness (early-harvest).
30545140	2	43	theme	commercial	549:558	arg1	early-harvest					570:582	early-harvest	570:582	early-harvest	570:582	This study investigated the extent to which commercial winemaking supplements (skin and seed tannins, and mannoprotein (MP)) can enhance the mouthfeel properties of red wine, in particular, wine made from grapes harvested before commercial ripeness (early-harvest).
30545140	2	43	theme	commercial	549:558	arg1	ripeness					560:567	commercial ripeness	549:567	commercial ripeness (early-harvest)	549:583	This study investigated the extent to which commercial winemaking supplements (skin and seed tannins, and mannoprotein (MP)) can enhance the mouthfeel properties of red wine, in particular, wine made from grapes harvested before commercial ripeness (early-harvest).
30545140	7	44	theme	increase	1323:1330	arg1	exception					1302:1310	the exception	1298:1310	the exception of a minor increase in sweetness	1298:1343	The DA panel perceived sensory differences between H1 and H2 wines, but could not perceive any effect of supplementation on wine mouthfeel properties, with the exception of a minor increase in sweetness, attributed to mannoprotein addition to H1 wines, even when MP was added to wines at 2.5 times the level recommended for use in Australia.
30545140	6	45	theme	oenotannin	1075:1084	arg1	addition					1058:1065	addition	1058:1065	addition of skin oenotannin	1058:1084	The addition of seed oenotannin yielded higher tannin levels than addition of skin oenotannin, particularly for wines made from early-harvest grapes.
30545140	5	46	theme	higher	876:881	arg1	levels					883:888	higher levels	876:888	higher levels of tannin	876:898	Wines made from riper grapes had higher levels of tannin than wines made from early-harvest grapes, but similar polysaccharide levels were observed.
30545140	0	47	theme	Products	49:56	arg1	Impact					0:5	Impact	0:5	Impact of Commercial Oenotannin and Mannoprotein Products on the Chemical and Sensory Properties of Shiraz Wines	0:111	Impact of Commercial Oenotannin and Mannoprotein Products on the Chemical and Sensory Properties of Shiraz Wines Made from Sequentially Harvested Fruit.
30545140	7	48	theme	H2	1200:1201	arg1	wines					1203:1207	H2 wines	1200:1207	H2 wines	1200:1207	The DA panel perceived sensory differences between H1 and H2 wines, but could not perceive any effect of supplementation on wine mouthfeel properties, with the exception of a minor increase in sweetness, attributed to mannoprotein addition to H1 wines, even when MP was added to wines at 2.5 times the level recommended for use in Australia.
30545140	4	49	theme	descriptive	802:812	arg1	DA					824:825	DA	824:825	DA	824:825	The chemical composition and mouthfeel properties of wines were then determined by high performance liquid chromatography and descriptive analysis (DA), respectively.
30545140	4	49	theme	descriptive	802:812	arg1	analysis					814:821	descriptive analysis	802:821	descriptive analysis (DA)	802:826	The chemical composition and mouthfeel properties of wines were then determined by high performance liquid chromatography and descriptive analysis (DA), respectively.
30545140	5	50	theme	similar	947:953	arg1	levels					970:975	similar polysaccharide levels	947:975	similar polysaccharide levels	947:975	Wines made from riper grapes had higher levels of tannin than wines made from early-harvest grapes, but similar polysaccharide levels were observed.
30545140	4	51	theme	high	759:762	arg1	chromatography					783:796	high performance liquid chromatography	759:796	high performance liquid chromatography	759:796	The chemical composition and mouthfeel properties of wines were then determined by high performance liquid chromatography and descriptive analysis (DA), respectively.
30545140	1	52	theme	wine	228:231	arg1	profiles					183:190	The tannin and polysaccharide profiles	153:190	profiles	183:190	The tannin and polysaccharide profiles and therefore sensory properties of wine are influenced by fruit maturity at harvest, and practices employed during winemaking.
30545140	1	52	theme	wine	228:231	arg1	properties					214:223	therefore sensory properties	196:223	therefore sensory properties of wine	196:231	The tannin and polysaccharide profiles and therefore sensory properties of wine are influenced by fruit maturity at harvest, and practices employed during winemaking.
30545140	7	53	from	use	1466:1468	arg1	Australia					1473:1481	Australia	1473:1481	Australia	1473:1481	The DA panel perceived sensory differences between H1 and H2 wines, but could not perceive any effect of supplementation on wine mouthfeel properties, with the exception of a minor increase in sweetness, attributed to mannoprotein addition to H1 wines, even when MP was added to wines at 2.5 times the level recommended for use in Australia.
30545140	3	54	theme	Shiraz	628:633	arg1	grapes					635:640	Shiraz grapes	628:640	Shiraz grapes harvested at 20.8 and 24.5 °Brix	628:673	Supplements were added to wines made from Shiraz grapes harvested at 20.8 and 24.5 °Brix.
30545140	2	55	dep	supplements	386:396	arg1	skin					399:402	skin	399:402	skin	399:402	This study investigated the extent to which commercial winemaking supplements (skin and seed tannins, and mannoprotein (MP)) can enhance the mouthfeel properties of red wine, in particular, wine made from grapes harvested before commercial ripeness (early-harvest).
30545140	2	55	dep	supplements	386:396	arg1	tannins					413:419	seed tannins	408:419	seed tannins	408:419	This study investigated the extent to which commercial winemaking supplements (skin and seed tannins, and mannoprotein (MP)) can enhance the mouthfeel properties of red wine, in particular, wine made from grapes harvested before commercial ripeness (early-harvest).
30545140	2	55	dep	supplements	386:396	arg1	mannoprotein					426:437	mannoprotein	426:437	mannoprotein (MP)	426:442	This study investigated the extent to which commercial winemaking supplements (skin and seed tannins, and mannoprotein (MP)) can enhance the mouthfeel properties of red wine, in particular, wine made from grapes harvested before commercial ripeness (early-harvest).
30545140	2	55	dep	supplements	386:396	arg1	MP					440:441	MP	440:441	MP	440:441	This study investigated the extent to which commercial winemaking supplements (skin and seed tannins, and mannoprotein (MP)) can enhance the mouthfeel properties of red wine, in particular, wine made from grapes harvested before commercial ripeness (early-harvest).
30545140	2	55	dep	supplements	386:396	arg1	supplements					386:396	commercial winemaking supplements	364:396	commercial winemaking supplements (skin and seed tannins, and mannoprotein (MP))	364:443	This study investigated the extent to which commercial winemaking supplements (skin and seed tannins, and mannoprotein (MP)) can enhance the mouthfeel properties of red wine, in particular, wine made from grapes harvested before commercial ripeness (early-harvest).
30545140	5	56	contain	had	872:874	arg2	levels					883:888	higher levels	876:888	higher levels of tannin	876:898	Wines made from riper grapes had higher levels of tannin than wines made from early-harvest grapes, but similar polysaccharide levels were observed.
30545140	5	56	contain	had	872:874	arg1	Wines					843:847	Wines	843:847	Wines made from riper grapes	843:870	Wines made from riper grapes had higher levels of tannin than wines made from early-harvest grapes, but similar polysaccharide levels were observed.
30545140	4	57	theme	chemical	680:687	arg1	composition					689:699	chemical composition	680:699	chemical composition	680:699	The chemical composition and mouthfeel properties of wines were then determined by high performance liquid chromatography and descriptive analysis (DA), respectively.
30545140	0	58	theme	Chemical	65:72	arg1	Properties					86:95	the Chemical and Sensory Properties	61:95	the Chemical and Sensory Properties of Shiraz Wines	61:111	Impact of Commercial Oenotannin and Mannoprotein Products on the Chemical and Sensory Properties of Shiraz Wines Made from Sequentially Harvested Fruit.
30545140	6	59	theme	skin	1070:1073	arg1	oenotannin					1075:1084	skin oenotannin	1070:1084	skin oenotannin	1070:1084	The addition of seed oenotannin yielded higher tannin levels than addition of skin oenotannin, particularly for wines made from early-harvest grapes.
30545140	4	60	theme	liquid	776:781	arg1	chromatography					783:796	high performance liquid chromatography	759:796	high performance liquid chromatography	759:796	The chemical composition and mouthfeel properties of wines were then determined by high performance liquid chromatography and descriptive analysis (DA), respectively.
30545140	7	61	theme	wine	1266:1269	arg1	properties					1281:1290	wine mouthfeel properties	1266:1290	wine mouthfeel properties	1266:1290	The DA panel perceived sensory differences between H1 and H2 wines, but could not perceive any effect of supplementation on wine mouthfeel properties, with the exception of a minor increase in sweetness, attributed to mannoprotein addition to H1 wines, even when MP was added to wines at 2.5 times the level recommended for use in Australia.
30545140	2	62	theme	winemaking	375:384	arg1	skin					399:402	skin	399:402	skin	399:402	This study investigated the extent to which commercial winemaking supplements (skin and seed tannins, and mannoprotein (MP)) can enhance the mouthfeel properties of red wine, in particular, wine made from grapes harvested before commercial ripeness (early-harvest).
30545140	2	62	theme	winemaking	375:384	arg1	supplements					386:396	commercial winemaking supplements	364:396	commercial winemaking supplements (skin and seed tannins, and mannoprotein (MP))	364:443	This study investigated the extent to which commercial winemaking supplements (skin and seed tannins, and mannoprotein (MP)) can enhance the mouthfeel properties of red wine, in particular, wine made from grapes harvested before commercial ripeness (early-harvest).
30545140	2	62	theme	winemaking	375:384	arg1	mannoprotein					426:437	mannoprotein	426:437	mannoprotein (MP)	426:442	This study investigated the extent to which commercial winemaking supplements (skin and seed tannins, and mannoprotein (MP)) can enhance the mouthfeel properties of red wine, in particular, wine made from grapes harvested before commercial ripeness (early-harvest).
30545140	4	63	theme	performance	764:774	arg1	chromatography					783:796	high performance liquid chromatography	759:796	high performance liquid chromatography	759:796	The chemical composition and mouthfeel properties of wines were then determined by high performance liquid chromatography and descriptive analysis (DA), respectively.
30545140	2	64	theme	commercial	364:373	arg1	skin					399:402	skin	399:402	skin	399:402	This study investigated the extent to which commercial winemaking supplements (skin and seed tannins, and mannoprotein (MP)) can enhance the mouthfeel properties of red wine, in particular, wine made from grapes harvested before commercial ripeness (early-harvest).
30545140	2	64	theme	commercial	364:373	arg1	supplements					386:396	commercial winemaking supplements	364:396	commercial winemaking supplements (skin and seed tannins, and mannoprotein (MP))	364:443	This study investigated the extent to which commercial winemaking supplements (skin and seed tannins, and mannoprotein (MP)) can enhance the mouthfeel properties of red wine, in particular, wine made from grapes harvested before commercial ripeness (early-harvest).
30545140	2	64	theme	commercial	364:373	arg1	mannoprotein					426:437	mannoprotein	426:437	mannoprotein (MP)	426:442	This study investigated the extent to which commercial winemaking supplements (skin and seed tannins, and mannoprotein (MP)) can enhance the mouthfeel properties of red wine, in particular, wine made from grapes harvested before commercial ripeness (early-harvest).
30296008	9	0	theme	geese	1544:1548	arg1	microbiota					1554:1563	The herbivorous greylag geese gut microbiota	1520:1563	The herbivorous greylag geese gut microbiota	1520:1563	The herbivorous greylag geese gut microbiota harbored more carbohydrate-active enzymes than omnivorous ruddy shelducks.
30296008	7	1	theme	613	1138:1140	arg1	%					1166:1166	approximately 70%	1150:1166	approximately 70%	1150:1166	A total of 613 genera (approximately 70%) were found to be present in both groups.
30296008	7	1	theme	613	1138:1140	arg1	genera					1142:1147	613 genera	1138:1147	613 genera (approximately 70%)	1138:1167	A total of 613 genera (approximately 70%) were found to be present in both groups.
30296008	2	2	theme	ruddy	316:320	arg1	shelducks					322:330	Wild greylag geese (Anser anser) and ruddy shelducks	279:330	Wild greylag geese (Anser anser) and ruddy shelducks (Tadorna ferruginea)	279:351	Wild greylag geese (Anser anser) and ruddy shelducks (Tadorna ferruginea), migrating along the central Asian flyway, appear to be one of the most popular species in the rare birds rearing industries of China.
30296008	2	2	theme	ruddy	316:320	arg1	ferruginea					341:350	ferruginea	341:350	ferruginea	341:350	Wild greylag geese (Anser anser) and ruddy shelducks (Tadorna ferruginea), migrating along the central Asian flyway, appear to be one of the most popular species in the rare birds rearing industries of China.
30296008	4	3	theme	bird	782:785	arg1	species					787:793	these two bird species	772:793	these two bird species	772:793	Here, for the first time, we compared gut metagenomes from greylag geese to ruddy shelducks and investigated the similarities and differences between these two bird species in detail.
30296008	11	4	theme	domestication	1956:1968	arg1	values					1931:1936	reference values	1921:1936	reference values of the artificial domestication of these birds	1921:1983	In addition to providing a better understanding of the composition and function of wild birds gut microbiome, this comparative study provides reference values of the artificial domestication of these birds.
30296008	9	5	theme	omnivorous	1612:1621	arg1	shelducks					1629:1637	omnivorous ruddy shelducks	1612:1637	omnivorous ruddy shelducks	1612:1637	The herbivorous greylag geese gut microbiota harbored more carbohydrate-active enzymes than omnivorous ruddy shelducks.
30296008	6	6	theme	ruddy	1053:1057	arg1	shelducks					1059:1067	ruddy shelducks	1053:1067	ruddy shelducks	1053:1067	Furthermore, between the two species, 12 bacterial genera were found to be more abundant in ruddy shelducks and 41 genera were significantly higher in greylag geese.
30296008	7	7	located	present	1186:1192	arg2	present					1186:1192	present	1186:1192	present	1186:1192	A total of 613 genera (approximately 70%) were found to be present in both groups.
30296008	7	7	located	present	1186:1192	arg2	total					1129:1133	A total	1127:1133	A total of 613 genera (approximately 70%)	1127:1167	A total of 613 genera (approximately 70%) were found to be present in both groups.
30296008	7	7	located	present	1186:1192	arg1	groups					1202:1207	both groups	1197:1207	both groups	1197:1207	A total of 613 genera (approximately 70%) were found to be present in both groups.
30296008	6	8	theme	bacterial	1002:1010	arg1	genera					1012:1017	12 bacterial genera	999:1017	12 bacterial genera	999:1017	Furthermore, between the two species, 12 bacterial genera were found to be more abundant in ruddy shelducks and 41 genera were significantly higher in greylag geese.
30296008	8	9	theme	amino	1323:1327	arg1	metabolism					1334:1343	amino acid metabolism	1323:1343	amino acid metabolism	1323:1343	Metabolic categories related to carbohydrate metabolism, metabolism of cofactors and vitamins, lipid metabolism, amino acid metabolism, and glycan biosynthesis and metabolism were significantly more abundant in ruddy shelducks, while greylag geese were enriched in nucleotide metabolism and energy metabolism.
30296008	9	10	theme	herbivorous	1524:1534	arg1	microbiota					1554:1563	The herbivorous greylag geese gut microbiota	1520:1563	The herbivorous greylag geese gut microbiota	1520:1563	The herbivorous greylag geese gut microbiota harbored more carbohydrate-active enzymes than omnivorous ruddy shelducks.
30296008	0	11	theme	Tadorna	104:110	arg1	shelducks					93:101	ruddy shelducks	87:101	ruddy shelducks (Tadorna ferruginea)	87:122	Comparative metagenomics of the gut microbiota in wild greylag geese (Anser anser) and ruddy shelducks (Tadorna ferruginea).
30296008	0	11	theme	Tadorna	104:110	arg1	ferruginea					112:121	Tadorna ferruginea	104:121	Tadorna ferruginea	104:121	Comparative metagenomics of the gut microbiota in wild greylag geese (Anser anser) and ruddy shelducks (Tadorna ferruginea).
30296008	11	12	theme	gut	1873:1875	arg1	microbiome					1877:1886	wild birds gut microbiome	1862:1886	wild birds gut microbiome	1862:1886	In addition to providing a better understanding of the composition and function of wild birds gut microbiome, this comparative study provides reference values of the artificial domestication of these birds.
30296008	4	13	theme	first	636:640	arg1	time					642:645	the first time	632:645	the first time	632:645	Here, for the first time, we compared gut metagenomes from greylag geese to ruddy shelducks and investigated the similarities and differences between these two bird species in detail.
30296008	11	14	theme	wild	1862:1865	arg1	microbiome					1877:1886	wild birds gut microbiome	1862:1886	wild birds gut microbiome	1862:1886	In addition to providing a better understanding of the composition and function of wild birds gut microbiome, this comparative study provides reference values of the artificial domestication of these birds.
30296008	3	15	theme	communities	545:555	arg1	function					515:522	function	515:522	function	515:522	However, the structure and function of the gut microbial communities associated with these two bird species remain poorly understood.
30296008	3	15	theme	communities	545:555	arg1	structure					501:509	structure	501:509	structure	501:509	However, the structure and function of the gut microbial communities associated with these two bird species remain poorly understood.
30296008	10	16	theme	antibiotic	1665:1674	arg1	categories					1687:1696	antibiotic resistance categories	1665:1696	antibiotic resistance categories	1665:1696	In our study, a range of antibiotic resistance categories were also identified in the gut microbiota of greylag geese and ruddy shelducks.
30296008	0	17	theme	greylag	55:61	arg1	geese					63:67	wild greylag geese	50:67	wild greylag geese (Anser anser)	50:81	Comparative metagenomics of the gut microbiota in wild greylag geese (Anser anser) and ruddy shelducks (Tadorna ferruginea).
30296008	0	17	theme	greylag	55:61	arg1	anser					76:80	anser	76:80	anser	76:80	Comparative metagenomics of the gut microbiota in wild greylag geese (Anser anser) and ruddy shelducks (Tadorna ferruginea).
30296008	3	18	theme	microbial	535:543	arg1	communities					545:555	the gut microbial communities	527:555	the gut microbial communities associated with these two bird species	527:594	However, the structure and function of the gut microbial communities associated with these two bird species remain poorly understood.
30296008	2	19	theme	popular	425:431	arg1	species					433:439	the most popular species	416:439	the most popular species	416:439	Wild greylag geese (Anser anser) and ruddy shelducks (Tadorna ferruginea), migrating along the central Asian flyway, appear to be one of the most popular species in the rare birds rearing industries of China.
30296008	3	20	dep	structure	501:509	arg1	the					497:499	the	497:499	the	497:499	However, the structure and function of the gut microbial communities associated with these two bird species remain poorly understood.
30296008	10	21	theme	greylag	1744:1750	arg1	geese					1752:1756	greylag geese	1744:1756	greylag geese	1744:1756	In our study, a range of antibiotic resistance categories were also identified in the gut microbiota of greylag geese and ruddy shelducks.
30296008	4	22	theme	greylag	681:687	arg1	geese					689:693	greylag geese	681:693	greylag geese to ruddy shelducks	681:712	Here, for the first time, we compared gut metagenomes from greylag geese to ruddy shelducks and investigated the similarities and differences between these two bird species in detail.
30296008	2	23	theme	rare	448:451	arg1	birds					453:457	the rare birds	444:457	the rare birds rearing industries of China	444:485	Wild greylag geese (Anser anser) and ruddy shelducks (Tadorna ferruginea), migrating along the central Asian flyway, appear to be one of the most popular species in the rare birds rearing industries of China.
30296008	11	24	theme	function	1850:1857	arg1	understanding					1813:1825	a better understanding	1804:1825	a better understanding of the composition and function of wild birds gut microbiome	1804:1886	In addition to providing a better understanding of the composition and function of wild birds gut microbiome, this comparative study provides reference values of the artificial domestication of these birds.
30296008	8	25	theme	ruddy	1421:1425	arg1	shelducks					1427:1435	ruddy shelducks	1421:1435	ruddy shelducks	1421:1435	Metabolic categories related to carbohydrate metabolism, metabolism of cofactors and vitamins, lipid metabolism, amino acid metabolism, and glycan biosynthesis and metabolism were significantly more abundant in ruddy shelducks, while greylag geese were enriched in nucleotide metabolism and energy metabolism.
30296008	0	26	theme	Comparative	0:10	arg1	metagenomics					12:23	Comparative metagenomics	0:23	Comparative metagenomics of the gut	0:34	Comparative metagenomics of the gut microbiota in wild greylag geese (Anser anser) and ruddy shelducks (Tadorna ferruginea).
30296008	10	27	theme	shelducks	1768:1776	arg1	microbiota					1730:1739	the gut microbiota	1722:1739	the gut microbiota of greylag geese and ruddy shelducks	1722:1776	In our study, a range of antibiotic resistance categories were also identified in the gut microbiota of greylag geese and ruddy shelducks.
30296008	11	28	theme	microbiome	1877:1886	arg1	composition					1834:1844	composition	1834:1844	composition	1834:1844	In addition to providing a better understanding of the composition and function of wild birds gut microbiome, this comparative study provides reference values of the artificial domestication of these birds.
30296008	11	28	theme	microbiome	1877:1886	arg1	function					1850:1857	function	1850:1857	function	1850:1857	In addition to providing a better understanding of the composition and function of wild birds gut microbiome, this comparative study provides reference values of the artificial domestication of these birds.
30296008	2	29	theme	Asian	382:386	arg1	flyway					388:393	the central Asian flyway	370:393	the central Asian flyway	370:393	Wild greylag geese (Anser anser) and ruddy shelducks (Tadorna ferruginea), migrating along the central Asian flyway, appear to be one of the most popular species in the rare birds rearing industries of China.
30296008	1	30	theme	system	271:276	arg1	development					249:259	the development	245:259	the development of immune system	245:276	Gut microbiome contributes to host health by maintaining homeostasis, increasing digestive efficiency, and facilitating the development of immune system.
30296008	11	31	dep	providing	1794:1802	arg1	addition					1782:1789	addition	1782:1789	addition	1782:1789	In addition to providing a better understanding of the composition and function of wild birds gut microbiome, this comparative study provides reference values of the artificial domestication of these birds.
30296008	8	32	theme	greylag	1444:1450	arg1	geese					1452:1456	greylag geese	1444:1456	greylag geese	1444:1456	Metabolic categories related to carbohydrate metabolism, metabolism of cofactors and vitamins, lipid metabolism, amino acid metabolism, and glycan biosynthesis and metabolism were significantly more abundant in ruddy shelducks, while greylag geese were enriched in nucleotide metabolism and energy metabolism.
30296008	3	33	theme	bird	583:586	arg1	species					588:594	these two bird species	573:594	these two bird species	573:594	However, the structure and function of the gut microbial communities associated with these two bird species remain poorly understood.
30296008	8	34	theme	cofactors	1281:1289	arg1	metabolism					1334:1343	amino acid metabolism	1323:1343	amino acid metabolism	1323:1343	Metabolic categories related to carbohydrate metabolism, metabolism of cofactors and vitamins, lipid metabolism, amino acid metabolism, and glycan biosynthesis and metabolism were significantly more abundant in ruddy shelducks, while greylag geese were enriched in nucleotide metabolism and energy metabolism.
30296008	8	34	theme	cofactors	1281:1289	arg1	metabolism					1255:1264	carbohydrate metabolism	1242:1264	carbohydrate metabolism	1242:1264	Metabolic categories related to carbohydrate metabolism, metabolism of cofactors and vitamins, lipid metabolism, amino acid metabolism, and glycan biosynthesis and metabolism were significantly more abundant in ruddy shelducks, while greylag geese were enriched in nucleotide metabolism and energy metabolism.
30296008	8	34	theme	cofactors	1281:1289	arg1	metabolism					1374:1383	metabolism	1374:1383	metabolism	1374:1383	Metabolic categories related to carbohydrate metabolism, metabolism of cofactors and vitamins, lipid metabolism, amino acid metabolism, and glycan biosynthesis and metabolism were significantly more abundant in ruddy shelducks, while greylag geese were enriched in nucleotide metabolism and energy metabolism.
30296008	8	34	theme	cofactors	1281:1289	arg1	metabolism					1267:1276	metabolism	1267:1276	metabolism of cofactors and vitamins	1267:1302	Metabolic categories related to carbohydrate metabolism, metabolism of cofactors and vitamins, lipid metabolism, amino acid metabolism, and glycan biosynthesis and metabolism were significantly more abundant in ruddy shelducks, while greylag geese were enriched in nucleotide metabolism and energy metabolism.
30296008	8	34	theme	cofactors	1281:1289	arg1	metabolism					1311:1320	lipid metabolism	1305:1320	lipid metabolism	1305:1320	Metabolic categories related to carbohydrate metabolism, metabolism of cofactors and vitamins, lipid metabolism, amino acid metabolism, and glycan biosynthesis and metabolism were significantly more abundant in ruddy shelducks, while greylag geese were enriched in nucleotide metabolism and energy metabolism.
30296008	8	34	theme	cofactors	1281:1289	arg1	biosynthesis					1357:1368	glycan biosynthesis	1350:1368	glycan biosynthesis	1350:1368	Metabolic categories related to carbohydrate metabolism, metabolism of cofactors and vitamins, lipid metabolism, amino acid metabolism, and glycan biosynthesis and metabolism were significantly more abundant in ruddy shelducks, while greylag geese were enriched in nucleotide metabolism and energy metabolism.
30296008	6	35	from	shelducks	1059:1067	arg1	abundant					1041:1048	abundant	1041:1048	abundant	1041:1048	Furthermore, between the two species, 12 bacterial genera were found to be more abundant in ruddy shelducks and 41 genera were significantly higher in greylag geese.
30296008	0	36	theme	gut	32:34	arg1	metagenomics					12:23	Comparative metagenomics	0:23	Comparative metagenomics of the gut	0:34	Comparative metagenomics of the gut microbiota in wild greylag geese (Anser anser) and ruddy shelducks (Tadorna ferruginea).
30296008	5	37	theme	ruddy	944:948	arg1	shelducks					950:958	ruddy shelducks	944:958	ruddy shelducks	944:958	Taxonomic classifications revealed the top three bacterial phyla, Firmicutes, Proteobacteria, and Fusobacteria, in both greylag geese and ruddy shelducks.
30296008	8	38	theme	vitamins	1295:1302	arg1	metabolism					1334:1343	amino acid metabolism	1323:1343	amino acid metabolism	1323:1343	Metabolic categories related to carbohydrate metabolism, metabolism of cofactors and vitamins, lipid metabolism, amino acid metabolism, and glycan biosynthesis and metabolism were significantly more abundant in ruddy shelducks, while greylag geese were enriched in nucleotide metabolism and energy metabolism.
30296008	8	38	theme	vitamins	1295:1302	arg1	metabolism					1255:1264	carbohydrate metabolism	1242:1264	carbohydrate metabolism	1242:1264	Metabolic categories related to carbohydrate metabolism, metabolism of cofactors and vitamins, lipid metabolism, amino acid metabolism, and glycan biosynthesis and metabolism were significantly more abundant in ruddy shelducks, while greylag geese were enriched in nucleotide metabolism and energy metabolism.
30296008	8	38	theme	vitamins	1295:1302	arg1	metabolism					1374:1383	metabolism	1374:1383	metabolism	1374:1383	Metabolic categories related to carbohydrate metabolism, metabolism of cofactors and vitamins, lipid metabolism, amino acid metabolism, and glycan biosynthesis and metabolism were significantly more abundant in ruddy shelducks, while greylag geese were enriched in nucleotide metabolism and energy metabolism.
30296008	8	38	theme	vitamins	1295:1302	arg1	metabolism					1267:1276	metabolism	1267:1276	metabolism of cofactors and vitamins	1267:1302	Metabolic categories related to carbohydrate metabolism, metabolism of cofactors and vitamins, lipid metabolism, amino acid metabolism, and glycan biosynthesis and metabolism were significantly more abundant in ruddy shelducks, while greylag geese were enriched in nucleotide metabolism and energy metabolism.
30296008	8	38	theme	vitamins	1295:1302	arg1	metabolism					1311:1320	lipid metabolism	1305:1320	lipid metabolism	1305:1320	Metabolic categories related to carbohydrate metabolism, metabolism of cofactors and vitamins, lipid metabolism, amino acid metabolism, and glycan biosynthesis and metabolism were significantly more abundant in ruddy shelducks, while greylag geese were enriched in nucleotide metabolism and energy metabolism.
30296008	8	38	theme	vitamins	1295:1302	arg1	biosynthesis					1357:1368	glycan biosynthesis	1350:1368	glycan biosynthesis	1350:1368	Metabolic categories related to carbohydrate metabolism, metabolism of cofactors and vitamins, lipid metabolism, amino acid metabolism, and glycan biosynthesis and metabolism were significantly more abundant in ruddy shelducks, while greylag geese were enriched in nucleotide metabolism and energy metabolism.
30296008	8	39	from	abundant	1409:1416	arg1	shelducks					1427:1435	ruddy shelducks	1421:1435	ruddy shelducks	1421:1435	Metabolic categories related to carbohydrate metabolism, metabolism of cofactors and vitamins, lipid metabolism, amino acid metabolism, and glycan biosynthesis and metabolism were significantly more abundant in ruddy shelducks, while greylag geese were enriched in nucleotide metabolism and energy metabolism.
30296008	8	40	theme	lipid	1305:1309	arg1	metabolism					1311:1320	lipid metabolism	1305:1320	lipid metabolism	1305:1320	Metabolic categories related to carbohydrate metabolism, metabolism of cofactors and vitamins, lipid metabolism, amino acid metabolism, and glycan biosynthesis and metabolism were significantly more abundant in ruddy shelducks, while greylag geese were enriched in nucleotide metabolism and energy metabolism.
30296008	11	41	dep	composition	1834:1844	arg1	the					1830:1832	the	1830:1832	the	1830:1832	In addition to providing a better understanding of the composition and function of wild birds gut microbiome, this comparative study provides reference values of the artificial domestication of these birds.
30296008	9	42	theme	greylag	1536:1542	arg1	microbiota					1554:1563	The herbivorous greylag geese gut microbiota	1520:1563	The herbivorous greylag geese gut microbiota	1520:1563	The herbivorous greylag geese gut microbiota harbored more carbohydrate-active enzymes than omnivorous ruddy shelducks.
30296008	7	43	from	groups	1202:1207	arg1	present					1186:1192	present	1186:1192	present	1186:1192	A total of 613 genera (approximately 70%) were found to be present in both groups.
30296008	7	43	from	groups	1202:1207	arg1	total					1129:1133	A total	1127:1133	A total of 613 genera (approximately 70%)	1127:1167	A total of 613 genera (approximately 70%) were found to be present in both groups.
30296008	8	44	theme	energy	1501:1506	arg1	metabolism					1508:1517	energy metabolism	1501:1517	energy metabolism	1501:1517	Metabolic categories related to carbohydrate metabolism, metabolism of cofactors and vitamins, lipid metabolism, amino acid metabolism, and glycan biosynthesis and metabolism were significantly more abundant in ruddy shelducks, while greylag geese were enriched in nucleotide metabolism and energy metabolism.
30296008	9	45	theme	gut	1550:1552	arg1	microbiota					1554:1563	The herbivorous greylag geese gut microbiota	1520:1563	The herbivorous greylag geese gut microbiota	1520:1563	The herbivorous greylag geese gut microbiota harbored more carbohydrate-active enzymes than omnivorous ruddy shelducks.
30296008	7	46	theme	genera	1142:1147	arg1	present					1186:1192	present	1186:1192	present	1186:1192	A total of 613 genera (approximately 70%) were found to be present in both groups.
30296008	7	46	theme	genera	1142:1147	arg1	total					1129:1133	A total	1127:1133	A total of 613 genera (approximately 70%)	1127:1167	A total of 613 genera (approximately 70%) were found to be present in both groups.
30296008	11	47	theme	reference	1921:1929	arg1	values					1931:1936	reference values	1921:1936	reference values of the artificial domestication of these birds	1921:1983	In addition to providing a better understanding of the composition and function of wild birds gut microbiome, this comparative study provides reference values of the artificial domestication of these birds.
30296008	9	48	theme	carbohydrate-active	1579:1597	arg1	enzymes					1599:1605	more carbohydrate-active enzymes	1574:1605	more carbohydrate-active enzymes than omnivorous ruddy shelducks	1574:1637	The herbivorous greylag geese gut microbiota harbored more carbohydrate-active enzymes than omnivorous ruddy shelducks.
30296008	11	49	theme	artificial	1945:1954	arg1	domestication					1956:1968	the artificial domestication	1941:1968	the artificial domestication of these birds	1941:1983	In addition to providing a better understanding of the composition and function of wild birds gut microbiome, this comparative study provides reference values of the artificial domestication of these birds.
30296008	1	50	theme	host	155:158	arg1	health					160:165	host health	155:165	host health	155:165	Gut microbiome contributes to host health by maintaining homeostasis, increasing digestive efficiency, and facilitating the development of immune system.
30296008	10	51	theme	categories	1687:1696	arg1	range					1656:1660	a range	1654:1660	a range of antibiotic resistance categories	1654:1696	In our study, a range of antibiotic resistance categories were also identified in the gut microbiota of greylag geese and ruddy shelducks.
30296008	2	52	theme	geese	292:296	arg1	shelducks					322:330	Wild greylag geese (Anser anser) and ruddy shelducks	279:330	Wild greylag geese (Anser anser) and ruddy shelducks (Tadorna ferruginea)	279:351	Wild greylag geese (Anser anser) and ruddy shelducks (Tadorna ferruginea), migrating along the central Asian flyway, appear to be one of the most popular species in the rare birds rearing industries of China.
30296008	2	52	theme	geese	292:296	arg1	ferruginea					341:350	ferruginea	341:350	ferruginea	341:350	Wild greylag geese (Anser anser) and ruddy shelducks (Tadorna ferruginea), migrating along the central Asian flyway, appear to be one of the most popular species in the rare birds rearing industries of China.
30296008	9	53	theme	ruddy	1623:1627	arg1	shelducks					1629:1637	omnivorous ruddy shelducks	1612:1637	omnivorous ruddy shelducks	1612:1637	The herbivorous greylag geese gut microbiota harbored more carbohydrate-active enzymes than omnivorous ruddy shelducks.
30296008	8	54	theme	acid	1329:1332	arg1	metabolism					1334:1343	amino acid metabolism	1323:1343	amino acid metabolism	1323:1343	Metabolic categories related to carbohydrate metabolism, metabolism of cofactors and vitamins, lipid metabolism, amino acid metabolism, and glycan biosynthesis and metabolism were significantly more abundant in ruddy shelducks, while greylag geese were enriched in nucleotide metabolism and energy metabolism.
30296008	11	55	theme	birds	1867:1871	arg1	microbiome					1877:1886	wild birds gut microbiome	1862:1886	wild birds gut microbiome	1862:1886	In addition to providing a better understanding of the composition and function of wild birds gut microbiome, this comparative study provides reference values of the artificial domestication of these birds.
30296008	4	56	dep	similarities	735:746	arg1	the					731:733	the	731:733	the	731:733	Here, for the first time, we compared gut metagenomes from greylag geese to ruddy shelducks and investigated the similarities and differences between these two bird species in detail.
30296008	5	57	theme	top	845:847	arg1	Proteobacteria					884:897	Proteobacteria	884:897	Proteobacteria	884:897	Taxonomic classifications revealed the top three bacterial phyla, Firmicutes, Proteobacteria, and Fusobacteria, in both greylag geese and ruddy shelducks.
30296008	5	57	theme	top	845:847	arg1	Fusobacteria					904:915	Fusobacteria	904:915	Fusobacteria	904:915	Taxonomic classifications revealed the top three bacterial phyla, Firmicutes, Proteobacteria, and Fusobacteria, in both greylag geese and ruddy shelducks.
30296008	5	57	theme	top	845:847	arg1	Firmicutes					872:881	Firmicutes	872:881	Firmicutes	872:881	Taxonomic classifications revealed the top three bacterial phyla, Firmicutes, Proteobacteria, and Fusobacteria, in both greylag geese and ruddy shelducks.
30296008	5	57	theme	top	845:847	arg1	phyla					865:869	the top three bacterial phyla	841:869	the top three bacterial phyla	841:869	Taxonomic classifications revealed the top three bacterial phyla, Firmicutes, Proteobacteria, and Fusobacteria, in both greylag geese and ruddy shelducks.
30296008	11	58	theme	birds	1979:1983	arg1	domestication					1956:1968	the artificial domestication	1941:1968	the artificial domestication of these birds	1941:1983	In addition to providing a better understanding of the composition and function of wild birds gut microbiome, this comparative study provides reference values of the artificial domestication of these birds.
30296008	8	59	theme	glycan	1350:1355	arg1	biosynthesis					1357:1368	glycan biosynthesis	1350:1368	glycan biosynthesis	1350:1368	Metabolic categories related to carbohydrate metabolism, metabolism of cofactors and vitamins, lipid metabolism, amino acid metabolism, and glycan biosynthesis and metabolism were significantly more abundant in ruddy shelducks, while greylag geese were enriched in nucleotide metabolism and energy metabolism.
30296008	10	60	theme	resistance	1676:1685	arg1	categories					1687:1696	antibiotic resistance categories	1665:1696	antibiotic resistance categories	1665:1696	In our study, a range of antibiotic resistance categories were also identified in the gut microbiota of greylag geese and ruddy shelducks.
30296008	10	61	theme	geese	1752:1756	arg1	microbiota					1730:1739	the gut microbiota	1722:1739	the gut microbiota of greylag geese and ruddy shelducks	1722:1776	In our study, a range of antibiotic resistance categories were also identified in the gut microbiota of greylag geese and ruddy shelducks.
30296008	5	62	theme	bacterial	855:863	arg1	Proteobacteria					884:897	Proteobacteria	884:897	Proteobacteria	884:897	Taxonomic classifications revealed the top three bacterial phyla, Firmicutes, Proteobacteria, and Fusobacteria, in both greylag geese and ruddy shelducks.
30296008	5	62	theme	bacterial	855:863	arg1	Fusobacteria					904:915	Fusobacteria	904:915	Fusobacteria	904:915	Taxonomic classifications revealed the top three bacterial phyla, Firmicutes, Proteobacteria, and Fusobacteria, in both greylag geese and ruddy shelducks.
30296008	5	62	theme	bacterial	855:863	arg1	Firmicutes					872:881	Firmicutes	872:881	Firmicutes	872:881	Taxonomic classifications revealed the top three bacterial phyla, Firmicutes, Proteobacteria, and Fusobacteria, in both greylag geese and ruddy shelducks.
30296008	5	62	theme	bacterial	855:863	arg1	phyla					865:869	the top three bacterial phyla	841:869	the top three bacterial phyla	841:869	Taxonomic classifications revealed the top three bacterial phyla, Firmicutes, Proteobacteria, and Fusobacteria, in both greylag geese and ruddy shelducks.
30296008	2	63	theme	greylag	284:290	arg1	geese					292:296	Wild greylag geese	279:296	Wild greylag geese (Anser anser)	279:310	Wild greylag geese (Anser anser) and ruddy shelducks (Tadorna ferruginea), migrating along the central Asian flyway, appear to be one of the most popular species in the rare birds rearing industries of China.
30296008	2	63	theme	greylag	284:290	arg1	anser					305:309	anser	305:309	anser	305:309	Wild greylag geese (Anser anser) and ruddy shelducks (Tadorna ferruginea), migrating along the central Asian flyway, appear to be one of the most popular species in the rare birds rearing industries of China.
30296008	6	64	theme	greylag	1112:1118	arg1	geese					1120:1124	greylag geese	1112:1124	greylag geese	1112:1124	Furthermore, between the two species, 12 bacterial genera were found to be more abundant in ruddy shelducks and 41 genera were significantly higher in greylag geese.
30296008	3	65	theme	gut	531:533	arg1	communities					545:555	the gut microbial communities	527:555	the gut microbial communities associated with these two bird species	527:594	However, the structure and function of the gut microbial communities associated with these two bird species remain poorly understood.
30296008	5	66	theme	Taxonomic	806:814	arg1	classifications					816:830	Taxonomic classifications	806:830	Taxonomic classifications	806:830	Taxonomic classifications revealed the top three bacterial phyla, Firmicutes, Proteobacteria, and Fusobacteria, in both greylag geese and ruddy shelducks.
30296008	2	67	theme	species	433:439	arg1	species					433:439	the most popular species	416:439	the most popular species	416:439	Wild greylag geese (Anser anser) and ruddy shelducks (Tadorna ferruginea), migrating along the central Asian flyway, appear to be one of the most popular species in the rare birds rearing industries of China.
30296008	2	67	theme	species	433:439	arg1	one					409:411	one	409:411	one	409:411	Wild greylag geese (Anser anser) and ruddy shelducks (Tadorna ferruginea), migrating along the central Asian flyway, appear to be one of the most popular species in the rare birds rearing industries of China.
30296008	1	68	theme	Gut	125:127	arg1	microbiome					129:138	Gut microbiome	125:138	Gut microbiome	125:138	Gut microbiome contributes to host health by maintaining homeostasis, increasing digestive efficiency, and facilitating the development of immune system.
30296008	2	69	theme	China	481:485	arg1	industries					467:476	industries	467:476	industries of China	467:485	Wild greylag geese (Anser anser) and ruddy shelducks (Tadorna ferruginea), migrating along the central Asian flyway, appear to be one of the most popular species in the rare birds rearing industries of China.
30296008	2	70	theme	Wild	279:282	arg1	geese					292:296	Wild greylag geese	279:296	Wild greylag geese (Anser anser)	279:310	Wild greylag geese (Anser anser) and ruddy shelducks (Tadorna ferruginea), migrating along the central Asian flyway, appear to be one of the most popular species in the rare birds rearing industries of China.
30296008	2	70	theme	Wild	279:282	arg1	anser					305:309	anser	305:309	anser	305:309	Wild greylag geese (Anser anser) and ruddy shelducks (Tadorna ferruginea), migrating along the central Asian flyway, appear to be one of the most popular species in the rare birds rearing industries of China.
30296008	2	71	from	one	409:411	arg1	birds					453:457	the rare birds	444:457	the rare birds rearing industries of China	444:485	Wild greylag geese (Anser anser) and ruddy shelducks (Tadorna ferruginea), migrating along the central Asian flyway, appear to be one of the most popular species in the rare birds rearing industries of China.
30296008	8	72	theme	Metabolic	1210:1218	arg1	categories					1220:1229	Metabolic categories	1210:1229	Metabolic categories related to carbohydrate metabolism, metabolism of cofactors and vitamins, lipid metabolism, amino acid metabolism, and glycan biosynthesis and metabolism	1210:1383	Metabolic categories related to carbohydrate metabolism, metabolism of cofactors and vitamins, lipid metabolism, amino acid metabolism, and glycan biosynthesis and metabolism were significantly more abundant in ruddy shelducks, while greylag geese were enriched in nucleotide metabolism and energy metabolism.
30296008	0	73	theme	ruddy	87:91	arg1	shelducks					93:101	ruddy shelducks	87:101	ruddy shelducks (Tadorna ferruginea)	87:122	Comparative metagenomics of the gut microbiota in wild greylag geese (Anser anser) and ruddy shelducks (Tadorna ferruginea).
30296008	0	73	theme	ruddy	87:91	arg1	ferruginea					112:121	Tadorna ferruginea	104:121	Tadorna ferruginea	104:121	Comparative metagenomics of the gut microbiota in wild greylag geese (Anser anser) and ruddy shelducks (Tadorna ferruginea).
30296008	0	74	from	microbiota	36:45	arg1	shelducks					93:101	ruddy shelducks	87:101	ruddy shelducks (Tadorna ferruginea)	87:122	Comparative metagenomics of the gut microbiota in wild greylag geese (Anser anser) and ruddy shelducks (Tadorna ferruginea).
30296008	0	74	from	microbiota	36:45	arg1	ferruginea					112:121	Tadorna ferruginea	104:121	Tadorna ferruginea	104:121	Comparative metagenomics of the gut microbiota in wild greylag geese (Anser anser) and ruddy shelducks (Tadorna ferruginea).
30296008	0	74	from	microbiota	36:45	arg1	geese					63:67	wild greylag geese	50:67	wild greylag geese (Anser anser)	50:81	Comparative metagenomics of the gut microbiota in wild greylag geese (Anser anser) and ruddy shelducks (Tadorna ferruginea).
30296008	0	74	from	microbiota	36:45	arg1	anser					76:80	anser	76:80	anser	76:80	Comparative metagenomics of the gut microbiota in wild greylag geese (Anser anser) and ruddy shelducks (Tadorna ferruginea).
30296008	8	75	theme	related	1231:1237	arg1	categories					1220:1229	Metabolic categories	1210:1229	Metabolic categories related to carbohydrate metabolism, metabolism of cofactors and vitamins, lipid metabolism, amino acid metabolism, and glycan biosynthesis and metabolism	1210:1383	Metabolic categories related to carbohydrate metabolism, metabolism of cofactors and vitamins, lipid metabolism, amino acid metabolism, and glycan biosynthesis and metabolism were significantly more abundant in ruddy shelducks, while greylag geese were enriched in nucleotide metabolism and energy metabolism.
30296008	8	76	theme	carbohydrate	1242:1253	arg1	metabolism					1255:1264	carbohydrate metabolism	1242:1264	carbohydrate metabolism	1242:1264	Metabolic categories related to carbohydrate metabolism, metabolism of cofactors and vitamins, lipid metabolism, amino acid metabolism, and glycan biosynthesis and metabolism were significantly more abundant in ruddy shelducks, while greylag geese were enriched in nucleotide metabolism and energy metabolism.
30296008	11	77	theme	better	1806:1811	arg1	understanding					1813:1825	a better understanding	1804:1825	a better understanding of the composition and function of wild birds gut microbiome	1804:1886	In addition to providing a better understanding of the composition and function of wild birds gut microbiome, this comparative study provides reference values of the artificial domestication of these birds.
30296008	1	78	theme	immune	264:269	arg1	system					271:276	immune system	264:276	immune system	264:276	Gut microbiome contributes to host health by maintaining homeostasis, increasing digestive efficiency, and facilitating the development of immune system.
30296008	10	79	theme	gut	1726:1728	arg1	microbiota					1730:1739	the gut microbiota	1722:1739	the gut microbiota of greylag geese and ruddy shelducks	1722:1776	In our study, a range of antibiotic resistance categories were also identified in the gut microbiota of greylag geese and ruddy shelducks.
30296008	7	80	attach	present	1186:1192	arg2	present					1186:1192	present	1186:1192	present	1186:1192	A total of 613 genera (approximately 70%) were found to be present in both groups.
30296008	7	80	attach	present	1186:1192	arg2	total					1129:1133	A total	1127:1133	A total of 613 genera (approximately 70%)	1127:1167	A total of 613 genera (approximately 70%) were found to be present in both groups.
30296008	7	80	attach	present	1186:1192	arg1	groups					1202:1207	both groups	1197:1207	both groups	1197:1207	A total of 613 genera (approximately 70%) were found to be present in both groups.
30296008	4	81	theme	gut	660:662	arg1	metagenomes					664:674	gut metagenomes	660:674	gut metagenomes	660:674	Here, for the first time, we compared gut metagenomes from greylag geese to ruddy shelducks and investigated the similarities and differences between these two bird species in detail.
30296008	2	82	theme	central	374:380	arg1	flyway					388:393	the central Asian flyway	370:393	the central Asian flyway	370:393	Wild greylag geese (Anser anser) and ruddy shelducks (Tadorna ferruginea), migrating along the central Asian flyway, appear to be one of the most popular species in the rare birds rearing industries of China.
30296008	8	83	from	shelducks	1427:1435	arg1	abundant					1409:1416	abundant	1409:1416	abundant	1409:1416	Metabolic categories related to carbohydrate metabolism, metabolism of cofactors and vitamins, lipid metabolism, amino acid metabolism, and glycan biosynthesis and metabolism were significantly more abundant in ruddy shelducks, while greylag geese were enriched in nucleotide metabolism and energy metabolism.
30296008	10	84	theme	ruddy	1762:1766	arg1	shelducks					1768:1776	ruddy shelducks	1762:1776	ruddy shelducks	1762:1776	In our study, a range of antibiotic resistance categories were also identified in the gut microbiota of greylag geese and ruddy shelducks.
30296008	11	85	theme	composition	1834:1844	arg1	understanding					1813:1825	a better understanding	1804:1825	a better understanding of the composition and function of wild birds gut microbiome	1804:1886	In addition to providing a better understanding of the composition and function of wild birds gut microbiome, this comparative study provides reference values of the artificial domestication of these birds.
30296008	0	86	theme	wild	50:53	arg1	geese					63:67	wild greylag geese	50:67	wild greylag geese (Anser anser)	50:81	Comparative metagenomics of the gut microbiota in wild greylag geese (Anser anser) and ruddy shelducks (Tadorna ferruginea).
30296008	0	86	theme	wild	50:53	arg1	anser					76:80	anser	76:80	anser	76:80	Comparative metagenomics of the gut microbiota in wild greylag geese (Anser anser) and ruddy shelducks (Tadorna ferruginea).
30296008	8	87	theme	nucleotide	1475:1484	arg1	metabolism					1486:1495	nucleotide metabolism	1475:1495	nucleotide metabolism	1475:1495	Metabolic categories related to carbohydrate metabolism, metabolism of cofactors and vitamins, lipid metabolism, amino acid metabolism, and glycan biosynthesis and metabolism were significantly more abundant in ruddy shelducks, while greylag geese were enriched in nucleotide metabolism and energy metabolism.
30296008	6	88	from	abundant	1041:1048	arg1	shelducks					1059:1067	ruddy shelducks	1053:1067	ruddy shelducks	1053:1067	Furthermore, between the two species, 12 bacterial genera were found to be more abundant in ruddy shelducks and 41 genera were significantly higher in greylag geese.
30296008	7	89	from	present	1186:1192	arg1	groups					1202:1207	both groups	1197:1207	both groups	1197:1207	A total of 613 genera (approximately 70%) were found to be present in both groups.
30296008	4	90	theme	ruddy	698:702	arg1	shelducks					704:712	ruddy shelducks	698:712	ruddy shelducks	698:712	Here, for the first time, we compared gut metagenomes from greylag geese to ruddy shelducks and investigated the similarities and differences between these two bird species in detail.
30296008	11	91	theme	comparative	1894:1904	arg1	study					1906:1910	this comparative study	1889:1910	this comparative study	1889:1910	In addition to providing a better understanding of the composition and function of wild birds gut microbiome, this comparative study provides reference values of the artificial domestication of these birds.
30296008	1	92	theme	digestive	206:214	arg1	efficiency					216:225	digestive efficiency	206:225	digestive efficiency	206:225	Gut microbiome contributes to host health by maintaining homeostasis, increasing digestive efficiency, and facilitating the development of immune system.
30296008	5	93	theme	greylag	926:932	arg1	geese					934:938	greylag geese	926:938	greylag geese	926:938	Taxonomic classifications revealed the top three bacterial phyla, Firmicutes, Proteobacteria, and Fusobacteria, in both greylag geese and ruddy shelducks.
30179696	3	0	theme	XANES	687:691	arg1	results					693:699	XANES results	687:699	XANES results	687:699	XANES results showed clear associations between the energy substrates and the changes in organic composition in terms of typical function groups (CO, CO and CN).
30179696	1	1	theme	Acidianus	440:448	arg1	manzaensis					450:459	thermoacidophilic archaeon Acidianus manzaensis YN-25	413:465	thermoacidophilic archaeon Acidianus manzaensis YN-25 acclimated with different energy substrates (FeS2, CuFeS2, S0, FeSO4)	413:535	For the first time, synchrotron radiation (SR) -based carbon K-edge X-ray absorption near edge structure (XANES) spectroscopy in-situ characterization was conducted to evaluate the evolution of superficial (about 10 nm) organic components of extracellular polymeric substances (EPS) of thermoacidophilic archaeon Acidianus manzaensis YN-25 acclimated with different energy substrates (FeS2, CuFeS2, S0, FeSO4).
30179696	6	2	with	consistent	1278:1287	arg1	trend					1298:1302	the trend	1294:1302	the trend in comparison with the results of FTIR and spectrophotometric determination	1294:1378	The results of linear-combination and peak fitting of the K-edge XANES for the extracellular superficial organic component C is consistent with the trend in comparison with the results of FTIR and spectrophotometric determination, but there are significant differences in the values.
30179696	2	3	theme	morphology	572:581	arg1	scanning					583:590	The atomic force microscopy (AFM) morphology scanning	538:590	The atomic force microscopy (AFM) morphology scanning	538:590	The atomic force microscopy (AFM) morphology scanning showed that the strain acclimated with different energy substrates varied a lot in EPS amount.
30179696	3	4	theme	clear	708:712	arg1	associations					714:725	clear associations	708:725	clear associations between the energy substrates and the changes in organic composition in terms of typical function groups (CO, CO and CN)	708:846	XANES results showed clear associations between the energy substrates and the changes in organic composition in terms of typical function groups (CO, CO and CN).
30179696	7	5	theme	measurement	1489:1499	arg1	depth					1501:1505	measurement depth	1489:1505	measurement depth	1489:1505	These differences are caused by the inconsistencies of measurement depth between XANES and the latter two characterization methods.
30179696	6	6	theme	peak	1188:1191	arg1	fitting					1193:1199	peak fitting	1188:1199	peak fitting	1188:1199	The results of linear-combination and peak fitting of the K-edge XANES for the extracellular superficial organic component C is consistent with the trend in comparison with the results of FTIR and spectrophotometric determination, but there are significant differences in the values.
30179696	6	7	theme	linear-combination	1165:1182	arg1	results					1154:1160	The results	1150:1160	The results of linear-combination and peak fitting of the K-edge XANES for the extracellular superficial organic component C is consistent with the trend in comparison with the results of FTIR and spectrophotometric determination, but there are significant differences in the values.	1150:1432	The results of linear-combination and peak fitting of the K-edge XANES for the extracellular superficial organic component C is consistent with the trend in comparison with the results of FTIR and spectrophotometric determination, but there are significant differences in the values.
30179696	3	8	theme	energy	739:744	arg1	substrates					746:755	the energy substrates	735:755	the energy substrates	735:755	XANES results showed clear associations between the energy substrates and the changes in organic composition in terms of typical function groups (CO, CO and CN).
30179696	1	9	theme	energy	493:498	arg1	substrates					500:509	different energy substrates	483:509	different energy substrates (FeS2, CuFeS2, S0, FeSO4)	483:535	For the first time, synchrotron radiation (SR) -based carbon K-edge X-ray absorption near edge structure (XANES) spectroscopy in-situ characterization was conducted to evaluate the evolution of superficial (about 10 nm) organic components of extracellular polymeric substances (EPS) of thermoacidophilic archaeon Acidianus manzaensis YN-25 acclimated with different energy substrates (FeS2, CuFeS2, S0, FeSO4).
30179696	1	10	theme	carbon	181:186	arg1	spectroscopy					240:251	synchrotron radiation (SR) -based carbon K-edge X-ray absorption near edge structure (XANES) spectroscopy	147:251	synchrotron radiation (SR) -based carbon K-edge X-ray absorption near edge structure (XANES) spectroscopy in-situ characterization	147:276	For the first time, synchrotron radiation (SR) -based carbon K-edge X-ray absorption near edge structure (XANES) spectroscopy in-situ characterization was conducted to evaluate the evolution of superficial (about 10 nm) organic components of extracellular polymeric substances (EPS) of thermoacidophilic archaeon Acidianus manzaensis YN-25 acclimated with different energy substrates (FeS2, CuFeS2, S0, FeSO4).
30179696	2	11	theme	atomic	542:547	arg1	microscopy					555:564	atomic force microscopy	542:564	The atomic force microscopy (AFM) morphology scanning	538:590	The atomic force microscopy (AFM) morphology scanning showed that the strain acclimated with different energy substrates varied a lot in EPS amount.
30179696	2	11	theme	atomic	542:547	arg1	AFM					567:569	AFM	567:569	AFM	567:569	The atomic force microscopy (AFM) morphology scanning showed that the strain acclimated with different energy substrates varied a lot in EPS amount.
30179696	5	12	theme	proteins	1067:1074	arg1	proportion					1053:1062	the highest proportion	1041:1062	the highest proportion of proteins	1041:1074	The FeSO4-acclimated cells contained the highest proportion of proteins, while the S0-acclimated cells contained more lipids and polysaccharides.
30179696	1	13	theme	X-ray	195:199	arg1	spectroscopy					240:251	synchrotron radiation (SR) -based carbon K-edge X-ray absorption near edge structure (XANES) spectroscopy	147:251	synchrotron radiation (SR) -based carbon K-edge X-ray absorption near edge structure (XANES) spectroscopy in-situ characterization	147:276	For the first time, synchrotron radiation (SR) -based carbon K-edge X-ray absorption near edge structure (XANES) spectroscopy in-situ characterization was conducted to evaluate the evolution of superficial (about 10 nm) organic components of extracellular polymeric substances (EPS) of thermoacidophilic archaeon Acidianus manzaensis YN-25 acclimated with different energy substrates (FeS2, CuFeS2, S0, FeSO4).
30179696	6	14	theme	organic	1255:1261	arg1	component					1263:1271	the extracellular superficial organic component	1225:1271	the extracellular superficial organic component C is consistent with the trend in comparison with the results of FTIR and spectrophotometric determination, but there are significant differences in the values	1225:1431	The results of linear-combination and peak fitting of the K-edge XANES for the extracellular superficial organic component C is consistent with the trend in comparison with the results of FTIR and spectrophotometric determination, but there are significant differences in the values.
30179696	1	15	theme	near	212:215	arg1	spectroscopy					240:251	synchrotron radiation (SR) -based carbon K-edge X-ray absorption near edge structure (XANES) spectroscopy	147:251	synchrotron radiation (SR) -based carbon K-edge X-ray absorption near edge structure (XANES) spectroscopy in-situ characterization	147:276	For the first time, synchrotron radiation (SR) -based carbon K-edge X-ray absorption near edge structure (XANES) spectroscopy in-situ characterization was conducted to evaluate the evolution of superficial (about 10 nm) organic components of extracellular polymeric substances (EPS) of thermoacidophilic archaeon Acidianus manzaensis YN-25 acclimated with different energy substrates (FeS2, CuFeS2, S0, FeSO4).
30179696	4	16	theme	chalcopyrite-	853:865	arg1	cells					889:893	The chalcopyrite- and pyrite-acclimated cells	849:893	The chalcopyrite- and pyrite-acclimated cells	849:893	The chalcopyrite- and pyrite-acclimated cells contained higher proportion of proteins but less proportion of polysaccharides than the S0-acclimated cells.
30179696	0	17	from	components	58:67	arg1	characterization					8:23	In situ characterization	0:23	In situ characterization of change in superficial organic components of thermoacidophilic archaeon Acidianus manzaensis YN-25.	0:125	In situ characterization of change in superficial organic components of thermoacidophilic archaeon Acidianus manzaensis YN-25.
30179696	6	18	theme	extracellular	1229:1241	arg1	component					1263:1271	the extracellular superficial organic component	1225:1271	the extracellular superficial organic component C is consistent with the trend in comparison with the results of FTIR and spectrophotometric determination, but there are significant differences in the values	1225:1431	The results of linear-combination and peak fitting of the K-edge XANES for the extracellular superficial organic component C is consistent with the trend in comparison with the results of FTIR and spectrophotometric determination, but there are significant differences in the values.
30179696	2	19	theme	microscopy	555:564	arg1	scanning					583:590	The atomic force microscopy (AFM) morphology scanning	538:590	The atomic force microscopy (AFM) morphology scanning	538:590	The atomic force microscopy (AFM) morphology scanning showed that the strain acclimated with different energy substrates varied a lot in EPS amount.
30179696	4	20	theme	pyrite-acclimated	871:887	arg1	cells					889:893	The chalcopyrite- and pyrite-acclimated cells	849:893	The chalcopyrite- and pyrite-acclimated cells	849:893	The chalcopyrite- and pyrite-acclimated cells contained higher proportion of proteins but less proportion of polysaccharides than the S0-acclimated cells.
30179696	2	21	theme	EPS	675:677	arg1	amount					679:684	EPS amount	675:684	EPS amount	675:684	The atomic force microscopy (AFM) morphology scanning showed that the strain acclimated with different energy substrates varied a lot in EPS amount.
30179696	4	22	dep	proportion	944:953	arg1	less					939:942	less	939:942	less	939:942	The chalcopyrite- and pyrite-acclimated cells contained higher proportion of proteins but less proportion of polysaccharides than the S0-acclimated cells.
30179696	1	23	theme	thermoacidophilic	413:429	arg1	manzaensis					450:459	thermoacidophilic archaeon Acidianus manzaensis YN-25	413:465	thermoacidophilic archaeon Acidianus manzaensis YN-25 acclimated with different energy substrates (FeS2, CuFeS2, S0, FeSO4)	413:535	For the first time, synchrotron radiation (SR) -based carbon K-edge X-ray absorption near edge structure (XANES) spectroscopy in-situ characterization was conducted to evaluate the evolution of superficial (about 10 nm) organic components of extracellular polymeric substances (EPS) of thermoacidophilic archaeon Acidianus manzaensis YN-25 acclimated with different energy substrates (FeS2, CuFeS2, S0, FeSO4).
30179696	1	24	dep	spectroscopy	240:251	arg1	characterization					261:276	in-situ characterization	253:276	synchrotron radiation (SR) -based carbon K-edge X-ray absorption near edge structure (XANES) spectroscopy in-situ characterization	147:276	For the first time, synchrotron radiation (SR) -based carbon K-edge X-ray absorption near edge structure (XANES) spectroscopy in-situ characterization was conducted to evaluate the evolution of superficial (about 10 nm) organic components of extracellular polymeric substances (EPS) of thermoacidophilic archaeon Acidianus manzaensis YN-25 acclimated with different energy substrates (FeS2, CuFeS2, S0, FeSO4).
30179696	6	25	theme	K-edge	1208:1213	arg1	XANES					1215:1219	the K-edge XANES	1204:1219	the K-edge XANES for the extracellular superficial organic component C is consistent with the trend in comparison with the results of FTIR and spectrophotometric determination, but there are significant differences in the values	1204:1431	The results of linear-combination and peak fitting of the K-edge XANES for the extracellular superficial organic component C is consistent with the trend in comparison with the results of FTIR and spectrophotometric determination, but there are significant differences in the values.
30179696	3	26	theme	organic	776:782	arg1	composition					784:794	organic composition	776:794	organic composition in terms of typical function groups (CO, CO and CN)	776:846	XANES results showed clear associations between the energy substrates and the changes in organic composition in terms of typical function groups (CO, CO and CN).
30179696	1	27	theme	synchrotron	147:157	arg1	radiation					159:167	synchrotron radiation	147:167	synchrotron radiation	147:167	For the first time, synchrotron radiation (SR) -based carbon K-edge X-ray absorption near edge structure (XANES) spectroscopy in-situ characterization was conducted to evaluate the evolution of superficial (about 10 nm) organic components of extracellular polymeric substances (EPS) of thermoacidophilic archaeon Acidianus manzaensis YN-25 acclimated with different energy substrates (FeS2, CuFeS2, S0, FeSO4).
30179696	0	28	from	characterization	8:23	arg1	components					58:67	superficial organic components	38:67	superficial organic components of thermoacidophilic archaeon Acidianus manzaensis YN-25	38:124	In situ characterization of change in superficial organic components of thermoacidophilic archaeon Acidianus manzaensis YN-25.
30179696	3	29	theme	function	816:823	arg1	CN					844:845	CN	844:845	CN	844:845	XANES results showed clear associations between the energy substrates and the changes in organic composition in terms of typical function groups (CO, CO and CN).
30179696	3	29	theme	function	816:823	arg1	CO					837:838	CO	837:838	CO	837:838	XANES results showed clear associations between the energy substrates and the changes in organic composition in terms of typical function groups (CO, CO and CN).
30179696	3	29	theme	function	816:823	arg1	CO					833:834	CO	833:834	CO	833:834	XANES results showed clear associations between the energy substrates and the changes in organic composition in terms of typical function groups (CO, CO and CN).
30179696	3	29	theme	function	816:823	arg1	groups					825:830	typical function groups	808:830	typical function groups (CO, CO and CN)	808:846	XANES results showed clear associations between the energy substrates and the changes in organic composition in terms of typical function groups (CO, CO and CN).
30179696	0	30	theme	thermoacidophilic	72:88	arg1	manzaensis					109:118	thermoacidophilic archaeon Acidianus manzaensis YN-25	72:124	thermoacidophilic archaeon Acidianus manzaensis YN-25	72:124	In situ characterization of change in superficial organic components of thermoacidophilic archaeon Acidianus manzaensis YN-25.
30179696	4	31	theme	proteins	926:933	arg1	proportion					912:921	higher proportion	905:921	higher proportion of proteins	905:933	The chalcopyrite- and pyrite-acclimated cells contained higher proportion of proteins but less proportion of polysaccharides than the S0-acclimated cells.
30179696	3	32	from	changes	765:771	arg1	composition					784:794	organic composition	776:794	organic composition in terms of typical function groups (CO, CO and CN)	776:846	XANES results showed clear associations between the energy substrates and the changes in organic composition in terms of typical function groups (CO, CO and CN).
30179696	2	33	theme	energy	641:646	arg1	substrates					648:657	different energy substrates	631:657	different energy substrates	631:657	The atomic force microscopy (AFM) morphology scanning showed that the strain acclimated with different energy substrates varied a lot in EPS amount.
30179696	0	34	theme	Acidianus	99:107	arg1	manzaensis					109:118	thermoacidophilic archaeon Acidianus manzaensis YN-25	72:124	thermoacidophilic archaeon Acidianus manzaensis YN-25	72:124	In situ characterization of change in superficial organic components of thermoacidophilic archaeon Acidianus manzaensis YN-25.
30179696	1	35	theme	organic	347:353	arg1	components					355:364	superficial (about 10 nm) organic components	321:364	superficial (about 10 nm) organic components	321:364	For the first time, synchrotron radiation (SR) -based carbon K-edge X-ray absorption near edge structure (XANES) spectroscopy in-situ characterization was conducted to evaluate the evolution of superficial (about 10 nm) organic components of extracellular polymeric substances (EPS) of thermoacidophilic archaeon Acidianus manzaensis YN-25 acclimated with different energy substrates (FeS2, CuFeS2, S0, FeSO4).
30179696	1	36	dep	superficial	321:331	arg1	10 nm					340:344	10 nm	340:344	10 nm	340:344	For the first time, synchrotron radiation (SR) -based carbon K-edge X-ray absorption near edge structure (XANES) spectroscopy in-situ characterization was conducted to evaluate the evolution of superficial (about 10 nm) organic components of extracellular polymeric substances (EPS) of thermoacidophilic archaeon Acidianus manzaensis YN-25 acclimated with different energy substrates (FeS2, CuFeS2, S0, FeSO4).
30179696	6	37	theme	determination	1366:1378	arg1	results					1327:1333	the results	1323:1333	the results of FTIR and spectrophotometric determination	1323:1378	The results of linear-combination and peak fitting of the K-edge XANES for the extracellular superficial organic component C is consistent with the trend in comparison with the results of FTIR and spectrophotometric determination, but there are significant differences in the values.
30179696	0	38	from	change	28:33	arg1	components					58:67	superficial organic components	38:67	superficial organic components of thermoacidophilic archaeon Acidianus manzaensis YN-25	38:124	In situ characterization of change in superficial organic components of thermoacidophilic archaeon Acidianus manzaensis YN-25.
30179696	0	39	theme	In	0:1	arg1	characterization					8:23	In situ characterization	0:23	In situ characterization of change in superficial organic components of thermoacidophilic archaeon Acidianus manzaensis YN-25.	0:125	In situ characterization of change in superficial organic components of thermoacidophilic archaeon Acidianus manzaensis YN-25.
30179696	1	40	theme	archaeon	431:438	arg1	manzaensis					450:459	thermoacidophilic archaeon Acidianus manzaensis YN-25	413:465	thermoacidophilic archaeon Acidianus manzaensis YN-25 acclimated with different energy substrates (FeS2, CuFeS2, S0, FeSO4)	413:535	For the first time, synchrotron radiation (SR) -based carbon K-edge X-ray absorption near edge structure (XANES) spectroscopy in-situ characterization was conducted to evaluate the evolution of superficial (about 10 nm) organic components of extracellular polymeric substances (EPS) of thermoacidophilic archaeon Acidianus manzaensis YN-25 acclimated with different energy substrates (FeS2, CuFeS2, S0, FeSO4).
30179696	1	41	theme	polymeric	383:391	arg1	EPS					405:407	EPS	405:407	EPS	405:407	For the first time, synchrotron radiation (SR) -based carbon K-edge X-ray absorption near edge structure (XANES) spectroscopy in-situ characterization was conducted to evaluate the evolution of superficial (about 10 nm) organic components of extracellular polymeric substances (EPS) of thermoacidophilic archaeon Acidianus manzaensis YN-25 acclimated with different energy substrates (FeS2, CuFeS2, S0, FeSO4).
30179696	1	41	theme	polymeric	383:391	arg1	substances					393:402	extracellular polymeric substances	369:402	extracellular polymeric substances (EPS) of thermoacidophilic archaeon Acidianus manzaensis YN-25 acclimated with different energy substrates (FeS2, CuFeS2, S0, FeSO4)	369:535	For the first time, synchrotron radiation (SR) -based carbon K-edge X-ray absorption near edge structure (XANES) spectroscopy in-situ characterization was conducted to evaluate the evolution of superficial (about 10 nm) organic components of extracellular polymeric substances (EPS) of thermoacidophilic archaeon Acidianus manzaensis YN-25 acclimated with different energy substrates (FeS2, CuFeS2, S0, FeSO4).
30179696	4	42	theme	S0-acclimated	983:995	arg1	cells					997:1001	the S0-acclimated cells	979:1001	the S0-acclimated cells	979:1001	The chalcopyrite- and pyrite-acclimated cells contained higher proportion of proteins but less proportion of polysaccharides than the S0-acclimated cells.
30179696	1	43	dep	-based	174:179	arg1	radiation					159:167	synchrotron radiation	147:167	synchrotron radiation	147:167	For the first time, synchrotron radiation (SR) -based carbon K-edge X-ray absorption near edge structure (XANES) spectroscopy in-situ characterization was conducted to evaluate the evolution of superficial (about 10 nm) organic components of extracellular polymeric substances (EPS) of thermoacidophilic archaeon Acidianus manzaensis YN-25 acclimated with different energy substrates (FeS2, CuFeS2, S0, FeSO4).
30179696	0	44	theme	change	28:33	arg1	characterization					8:23	In situ characterization	0:23	In situ characterization of change in superficial organic components of thermoacidophilic archaeon Acidianus manzaensis YN-25.	0:125	In situ characterization of change in superficial organic components of thermoacidophilic archaeon Acidianus manzaensis YN-25.
30179696	5	45	theme	FeSO4-acclimated	1008:1023	arg1	cells					1025:1029	The FeSO4-acclimated cells	1004:1029	The FeSO4-acclimated cells	1004:1029	The FeSO4-acclimated cells contained the highest proportion of proteins, while the S0-acclimated cells contained more lipids and polysaccharides.
30179696	5	46	dep	more	1117:1120	arg1	polysaccharides					1133:1147	polysaccharides	1133:1147	polysaccharides	1133:1147	The FeSO4-acclimated cells contained the highest proportion of proteins, while the S0-acclimated cells contained more lipids and polysaccharides.
30179696	5	46	dep	more	1117:1120	arg1	lipids					1122:1127	lipids	1122:1127	lipids	1122:1127	The FeSO4-acclimated cells contained the highest proportion of proteins, while the S0-acclimated cells contained more lipids and polysaccharides.
30179696	0	47	theme	superficial	38:48	arg1	components					58:67	superficial organic components	38:67	superficial organic components of thermoacidophilic archaeon Acidianus manzaensis YN-25	38:124	In situ characterization of change in superficial organic components of thermoacidophilic archaeon Acidianus manzaensis YN-25.
30179696	5	48	contain	contained	1031:1039	arg1	cells					1025:1029	The FeSO4-acclimated cells	1004:1029	The FeSO4-acclimated cells	1004:1029	The FeSO4-acclimated cells contained the highest proportion of proteins, while the S0-acclimated cells contained more lipids and polysaccharides.
30179696	5	48	contain	contained	1031:1039	arg2	proportion					1053:1062	the highest proportion	1041:1062	the highest proportion of proteins	1041:1074	The FeSO4-acclimated cells contained the highest proportion of proteins, while the S0-acclimated cells contained more lipids and polysaccharides.
30179696	1	49	theme	edge	217:220	arg1	spectroscopy					240:251	synchrotron radiation (SR) -based carbon K-edge X-ray absorption near edge structure (XANES) spectroscopy	147:251	synchrotron radiation (SR) -based carbon K-edge X-ray absorption near edge structure (XANES) spectroscopy in-situ characterization	147:276	For the first time, synchrotron radiation (SR) -based carbon K-edge X-ray absorption near edge structure (XANES) spectroscopy in-situ characterization was conducted to evaluate the evolution of superficial (about 10 nm) organic components of extracellular polymeric substances (EPS) of thermoacidophilic archaeon Acidianus manzaensis YN-25 acclimated with different energy substrates (FeS2, CuFeS2, S0, FeSO4).
30179696	6	50	dep	component	1263:1271	arg1	consistent					1278:1287	consistent	1278:1287	consistent	1278:1287	The results of linear-combination and peak fitting of the K-edge XANES for the extracellular superficial organic component C is consistent with the trend in comparison with the results of FTIR and spectrophotometric determination, but there are significant differences in the values.
30179696	6	50	dep	component	1263:1271	arg1	are					1391:1393	are	1391:1393	are significant differences in the values	1391:1431	The results of linear-combination and peak fitting of the K-edge XANES for the extracellular superficial organic component C is consistent with the trend in comparison with the results of FTIR and spectrophotometric determination, but there are significant differences in the values.
30179696	0	51	dep	In	0:1	arg1	situ					3:6	situ	3:6	situ	3:6	In situ characterization of change in superficial organic components of thermoacidophilic archaeon Acidianus manzaensis YN-25.
30179696	6	52	theme	significant	1395:1405	arg1	differences					1407:1417	significant differences	1395:1417	significant differences in the values	1395:1431	The results of linear-combination and peak fitting of the K-edge XANES for the extracellular superficial organic component C is consistent with the trend in comparison with the results of FTIR and spectrophotometric determination, but there are significant differences in the values.
30179696	7	53	theme	depth	1501:1505	arg1	inconsistencies					1470:1484	the inconsistencies	1466:1484	the inconsistencies of measurement depth between XANES and the latter two characterization methods	1466:1563	These differences are caused by the inconsistencies of measurement depth between XANES and the latter two characterization methods.
30179696	1	54	theme	manzaensis	450:459	arg1	EPS					405:407	EPS	405:407	EPS	405:407	For the first time, synchrotron radiation (SR) -based carbon K-edge X-ray absorption near edge structure (XANES) spectroscopy in-situ characterization was conducted to evaluate the evolution of superficial (about 10 nm) organic components of extracellular polymeric substances (EPS) of thermoacidophilic archaeon Acidianus manzaensis YN-25 acclimated with different energy substrates (FeS2, CuFeS2, S0, FeSO4).
30179696	1	54	theme	manzaensis	450:459	arg1	substances					393:402	extracellular polymeric substances	369:402	extracellular polymeric substances (EPS) of thermoacidophilic archaeon Acidianus manzaensis YN-25 acclimated with different energy substrates (FeS2, CuFeS2, S0, FeSO4)	369:535	For the first time, synchrotron radiation (SR) -based carbon K-edge X-ray absorption near edge structure (XANES) spectroscopy in-situ characterization was conducted to evaluate the evolution of superficial (about 10 nm) organic components of extracellular polymeric substances (EPS) of thermoacidophilic archaeon Acidianus manzaensis YN-25 acclimated with different energy substrates (FeS2, CuFeS2, S0, FeSO4).
30179696	1	55	theme	in-situ	253:259	arg1	characterization					261:276	in-situ characterization	253:276	synchrotron radiation (SR) -based carbon K-edge X-ray absorption near edge structure (XANES) spectroscopy in-situ characterization	147:276	For the first time, synchrotron radiation (SR) -based carbon K-edge X-ray absorption near edge structure (XANES) spectroscopy in-situ characterization was conducted to evaluate the evolution of superficial (about 10 nm) organic components of extracellular polymeric substances (EPS) of thermoacidophilic archaeon Acidianus manzaensis YN-25 acclimated with different energy substrates (FeS2, CuFeS2, S0, FeSO4).
30179696	6	56	theme	fitting	1193:1199	arg1	results					1154:1160	The results	1150:1160	The results of linear-combination and peak fitting of the K-edge XANES for the extracellular superficial organic component C is consistent with the trend in comparison with the results of FTIR and spectrophotometric determination, but there are significant differences in the values.	1150:1432	The results of linear-combination and peak fitting of the K-edge XANES for the extracellular superficial organic component C is consistent with the trend in comparison with the results of FTIR and spectrophotometric determination, but there are significant differences in the values.
30179696	1	57	theme	different	483:491	arg1	substrates					500:509	different energy substrates	483:509	different energy substrates (FeS2, CuFeS2, S0, FeSO4)	483:535	For the first time, synchrotron radiation (SR) -based carbon K-edge X-ray absorption near edge structure (XANES) spectroscopy in-situ characterization was conducted to evaluate the evolution of superficial (about 10 nm) organic components of extracellular polymeric substances (EPS) of thermoacidophilic archaeon Acidianus manzaensis YN-25 acclimated with different energy substrates (FeS2, CuFeS2, S0, FeSO4).
30179696	1	58	theme	-based	174:179	arg1	spectroscopy					240:251	synchrotron radiation (SR) -based carbon K-edge X-ray absorption near edge structure (XANES) spectroscopy	147:251	synchrotron radiation (SR) -based carbon K-edge X-ray absorption near edge structure (XANES) spectroscopy in-situ characterization	147:276	For the first time, synchrotron radiation (SR) -based carbon K-edge X-ray absorption near edge structure (XANES) spectroscopy in-situ characterization was conducted to evaluate the evolution of superficial (about 10 nm) organic components of extracellular polymeric substances (EPS) of thermoacidophilic archaeon Acidianus manzaensis YN-25 acclimated with different energy substrates (FeS2, CuFeS2, S0, FeSO4).
30179696	6	59	theme	spectrophotometric	1347:1364	arg1	determination					1366:1378	FTIR and spectrophotometric determination	1338:1378	FTIR and spectrophotometric determination	1338:1378	The results of linear-combination and peak fitting of the K-edge XANES for the extracellular superficial organic component C is consistent with the trend in comparison with the results of FTIR and spectrophotometric determination, but there are significant differences in the values.
30179696	6	60	with	comparison	1307:1316	arg1	results					1327:1333	the results	1323:1333	the results of FTIR and spectrophotometric determination	1323:1378	The results of linear-combination and peak fitting of the K-edge XANES for the extracellular superficial organic component C is consistent with the trend in comparison with the results of FTIR and spectrophotometric determination, but there are significant differences in the values.
30179696	1	61	theme	K-edge	188:193	arg1	spectroscopy					240:251	synchrotron radiation (SR) -based carbon K-edge X-ray absorption near edge structure (XANES) spectroscopy	147:251	synchrotron radiation (SR) -based carbon K-edge X-ray absorption near edge structure (XANES) spectroscopy in-situ characterization	147:276	For the first time, synchrotron radiation (SR) -based carbon K-edge X-ray absorption near edge structure (XANES) spectroscopy in-situ characterization was conducted to evaluate the evolution of superficial (about 10 nm) organic components of extracellular polymeric substances (EPS) of thermoacidophilic archaeon Acidianus manzaensis YN-25 acclimated with different energy substrates (FeS2, CuFeS2, S0, FeSO4).
30179696	6	62	theme	FTIR	1338:1341	arg1	determination					1366:1378	FTIR and spectrophotometric determination	1338:1378	FTIR and spectrophotometric determination	1338:1378	The results of linear-combination and peak fitting of the K-edge XANES for the extracellular superficial organic component C is consistent with the trend in comparison with the results of FTIR and spectrophotometric determination, but there are significant differences in the values.
30179696	1	63	theme	substances	393:402	arg1	evolution					308:316	the evolution	304:316	the evolution of superficial (about 10 nm) organic components of extracellular polymeric substances (EPS) of thermoacidophilic archaeon Acidianus manzaensis YN-25 acclimated with different energy substrates (FeS2, CuFeS2, S0, FeSO4)	304:535	For the first time, synchrotron radiation (SR) -based carbon K-edge X-ray absorption near edge structure (XANES) spectroscopy in-situ characterization was conducted to evaluate the evolution of superficial (about 10 nm) organic components of extracellular polymeric substances (EPS) of thermoacidophilic archaeon Acidianus manzaensis YN-25 acclimated with different energy substrates (FeS2, CuFeS2, S0, FeSO4).
30179696	1	64	theme	absorption	201:210	arg1	spectroscopy					240:251	synchrotron radiation (SR) -based carbon K-edge X-ray absorption near edge structure (XANES) spectroscopy	147:251	synchrotron radiation (SR) -based carbon K-edge X-ray absorption near edge structure (XANES) spectroscopy in-situ characterization	147:276	For the first time, synchrotron radiation (SR) -based carbon K-edge X-ray absorption near edge structure (XANES) spectroscopy in-situ characterization was conducted to evaluate the evolution of superficial (about 10 nm) organic components of extracellular polymeric substances (EPS) of thermoacidophilic archaeon Acidianus manzaensis YN-25 acclimated with different energy substrates (FeS2, CuFeS2, S0, FeSO4).
30179696	4	65	theme	higher	905:910	arg1	proportion					912:921	higher proportion	905:921	higher proportion of proteins	905:933	The chalcopyrite- and pyrite-acclimated cells contained higher proportion of proteins but less proportion of polysaccharides than the S0-acclimated cells.
30179696	6	66	theme	superficial	1243:1253	arg1	component					1263:1271	the extracellular superficial organic component	1225:1271	the extracellular superficial organic component C is consistent with the trend in comparison with the results of FTIR and spectrophotometric determination, but there are significant differences in the values	1225:1431	The results of linear-combination and peak fitting of the K-edge XANES for the extracellular superficial organic component C is consistent with the trend in comparison with the results of FTIR and spectrophotometric determination, but there are significant differences in the values.
30179696	5	67	theme	highest	1045:1051	arg1	proportion					1053:1062	the highest proportion	1041:1062	the highest proportion of proteins	1041:1074	The FeSO4-acclimated cells contained the highest proportion of proteins, while the S0-acclimated cells contained more lipids and polysaccharides.
30179696	1	68	theme	first	135:139	arg1	time					141:144	the first time	131:144	the first time	131:144	For the first time, synchrotron radiation (SR) -based carbon K-edge X-ray absorption near edge structure (XANES) spectroscopy in-situ characterization was conducted to evaluate the evolution of superficial (about 10 nm) organic components of extracellular polymeric substances (EPS) of thermoacidophilic archaeon Acidianus manzaensis YN-25 acclimated with different energy substrates (FeS2, CuFeS2, S0, FeSO4).
30179696	2	69	theme	force	549:553	arg1	microscopy					555:564	atomic force microscopy	542:564	The atomic force microscopy (AFM) morphology scanning	538:590	The atomic force microscopy (AFM) morphology scanning showed that the strain acclimated with different energy substrates varied a lot in EPS amount.
30179696	2	69	theme	force	549:553	arg1	AFM					567:569	AFM	567:569	AFM	567:569	The atomic force microscopy (AFM) morphology scanning showed that the strain acclimated with different energy substrates varied a lot in EPS amount.
30179696	6	70	theme	XANES	1215:1219	arg1	linear-combination					1165:1182	linear-combination	1165:1182	linear-combination	1165:1182	The results of linear-combination and peak fitting of the K-edge XANES for the extracellular superficial organic component C is consistent with the trend in comparison with the results of FTIR and spectrophotometric determination, but there are significant differences in the values.
30179696	6	70	theme	XANES	1215:1219	arg1	fitting					1193:1199	peak fitting	1188:1199	peak fitting	1188:1199	The results of linear-combination and peak fitting of the K-edge XANES for the extracellular superficial organic component C is consistent with the trend in comparison with the results of FTIR and spectrophotometric determination, but there are significant differences in the values.
30179696	3	71	dep	groups	825:830	arg1	CN					844:845	CN	844:845	CN	844:845	XANES results showed clear associations between the energy substrates and the changes in organic composition in terms of typical function groups (CO, CO and CN).
30179696	3	71	dep	groups	825:830	arg1	CO					837:838	CO	837:838	CO	837:838	XANES results showed clear associations between the energy substrates and the changes in organic composition in terms of typical function groups (CO, CO and CN).
30179696	3	71	dep	groups	825:830	arg1	CO					833:834	CO	833:834	CO	833:834	XANES results showed clear associations between the energy substrates and the changes in organic composition in terms of typical function groups (CO, CO and CN).
30179696	3	71	dep	groups	825:830	arg1	groups					825:830	typical function groups	808:830	typical function groups (CO, CO and CN)	808:846	XANES results showed clear associations between the energy substrates and the changes in organic composition in terms of typical function groups (CO, CO and CN).
30179696	0	72	theme	archaeon	90:97	arg1	manzaensis					109:118	thermoacidophilic archaeon Acidianus manzaensis YN-25	72:124	thermoacidophilic archaeon Acidianus manzaensis YN-25	72:124	In situ characterization of change in superficial organic components of thermoacidophilic archaeon Acidianus manzaensis YN-25.
30179696	1	73	dep	substrates	500:509	arg1	S0					526:527	S0	526:527	S0	526:527	For the first time, synchrotron radiation (SR) -based carbon K-edge X-ray absorption near edge structure (XANES) spectroscopy in-situ characterization was conducted to evaluate the evolution of superficial (about 10 nm) organic components of extracellular polymeric substances (EPS) of thermoacidophilic archaeon Acidianus manzaensis YN-25 acclimated with different energy substrates (FeS2, CuFeS2, S0, FeSO4).
30179696	1	73	dep	substrates	500:509	arg1	CuFeS2					518:523	CuFeS2	518:523	CuFeS2	518:523	For the first time, synchrotron radiation (SR) -based carbon K-edge X-ray absorption near edge structure (XANES) spectroscopy in-situ characterization was conducted to evaluate the evolution of superficial (about 10 nm) organic components of extracellular polymeric substances (EPS) of thermoacidophilic archaeon Acidianus manzaensis YN-25 acclimated with different energy substrates (FeS2, CuFeS2, S0, FeSO4).
30179696	1	73	dep	substrates	500:509	arg1	FeS2					512:515	FeS2	512:515	FeS2	512:515	For the first time, synchrotron radiation (SR) -based carbon K-edge X-ray absorption near edge structure (XANES) spectroscopy in-situ characterization was conducted to evaluate the evolution of superficial (about 10 nm) organic components of extracellular polymeric substances (EPS) of thermoacidophilic archaeon Acidianus manzaensis YN-25 acclimated with different energy substrates (FeS2, CuFeS2, S0, FeSO4).
30179696	1	73	dep	substrates	500:509	arg1	FeSO4					530:534	FeSO4	530:534	FeSO4	530:534	For the first time, synchrotron radiation (SR) -based carbon K-edge X-ray absorption near edge structure (XANES) spectroscopy in-situ characterization was conducted to evaluate the evolution of superficial (about 10 nm) organic components of extracellular polymeric substances (EPS) of thermoacidophilic archaeon Acidianus manzaensis YN-25 acclimated with different energy substrates (FeS2, CuFeS2, S0, FeSO4).
30179696	4	74	theme	polysaccharides	958:972	arg1	proportion					944:953	proportion	944:953	proportion of polysaccharides than the S0-acclimated cells	944:1001	The chalcopyrite- and pyrite-acclimated cells contained higher proportion of proteins but less proportion of polysaccharides than the S0-acclimated cells.
30179696	2	75	from	lot	668:670	arg1	amount					679:684	EPS amount	675:684	EPS amount	675:684	The atomic force microscopy (AFM) morphology scanning showed that the strain acclimated with different energy substrates varied a lot in EPS amount.
30179696	3	76	theme	groups	825:830	arg1	terms					799:803	terms	799:803	terms of typical function groups (CO, CO and CN)	799:846	XANES results showed clear associations between the energy substrates and the changes in organic composition in terms of typical function groups (CO, CO and CN).
30179696	0	77	theme	manzaensis	109:118	arg1	components					58:67	superficial organic components	38:67	superficial organic components of thermoacidophilic archaeon Acidianus manzaensis YN-25	38:124	In situ characterization of change in superficial organic components of thermoacidophilic archaeon Acidianus manzaensis YN-25.
30179696	6	78	from	differences	1407:1417	arg1	values					1426:1431	the values	1422:1431	the values	1422:1431	The results of linear-combination and peak fitting of the K-edge XANES for the extracellular superficial organic component C is consistent with the trend in comparison with the results of FTIR and spectrophotometric determination, but there are significant differences in the values.
30179696	5	79	contain	contained	1107:1115	arg2	more					1117:1120	more	1117:1120	more	1117:1120	The FeSO4-acclimated cells contained the highest proportion of proteins, while the S0-acclimated cells contained more lipids and polysaccharides.
30179696	5	79	contain	contained	1107:1115	arg1	cells					1101:1105	the S0-acclimated cells	1083:1105	the S0-acclimated cells	1083:1105	The FeSO4-acclimated cells contained the highest proportion of proteins, while the S0-acclimated cells contained more lipids and polysaccharides.
30179696	2	80	theme	different	631:639	arg1	substrates					648:657	different energy substrates	631:657	different energy substrates	631:657	The atomic force microscopy (AFM) morphology scanning showed that the strain acclimated with different energy substrates varied a lot in EPS amount.
30179696	3	81	theme	typical	808:814	arg1	CN					844:845	CN	844:845	CN	844:845	XANES results showed clear associations between the energy substrates and the changes in organic composition in terms of typical function groups (CO, CO and CN).
30179696	3	81	theme	typical	808:814	arg1	CO					837:838	CO	837:838	CO	837:838	XANES results showed clear associations between the energy substrates and the changes in organic composition in terms of typical function groups (CO, CO and CN).
30179696	3	81	theme	typical	808:814	arg1	CO					833:834	CO	833:834	CO	833:834	XANES results showed clear associations between the energy substrates and the changes in organic composition in terms of typical function groups (CO, CO and CN).
30179696	3	81	theme	typical	808:814	arg1	groups					825:830	typical function groups	808:830	typical function groups (CO, CO and CN)	808:846	XANES results showed clear associations between the energy substrates and the changes in organic composition in terms of typical function groups (CO, CO and CN).
30179696	1	82	theme	components	355:364	arg1	evolution					308:316	the evolution	304:316	the evolution of superficial (about 10 nm) organic components of extracellular polymeric substances (EPS) of thermoacidophilic archaeon Acidianus manzaensis YN-25 acclimated with different energy substrates (FeS2, CuFeS2, S0, FeSO4)	304:535	For the first time, synchrotron radiation (SR) -based carbon K-edge X-ray absorption near edge structure (XANES) spectroscopy in-situ characterization was conducted to evaluate the evolution of superficial (about 10 nm) organic components of extracellular polymeric substances (EPS) of thermoacidophilic archaeon Acidianus manzaensis YN-25 acclimated with different energy substrates (FeS2, CuFeS2, S0, FeSO4).
30179696	1	83	theme	extracellular	369:381	arg1	EPS					405:407	EPS	405:407	EPS	405:407	For the first time, synchrotron radiation (SR) -based carbon K-edge X-ray absorption near edge structure (XANES) spectroscopy in-situ characterization was conducted to evaluate the evolution of superficial (about 10 nm) organic components of extracellular polymeric substances (EPS) of thermoacidophilic archaeon Acidianus manzaensis YN-25 acclimated with different energy substrates (FeS2, CuFeS2, S0, FeSO4).
30179696	1	83	theme	extracellular	369:381	arg1	substances					393:402	extracellular polymeric substances	369:402	extracellular polymeric substances (EPS) of thermoacidophilic archaeon Acidianus manzaensis YN-25 acclimated with different energy substrates (FeS2, CuFeS2, S0, FeSO4)	369:535	For the first time, synchrotron radiation (SR) -based carbon K-edge X-ray absorption near edge structure (XANES) spectroscopy in-situ characterization was conducted to evaluate the evolution of superficial (about 10 nm) organic components of extracellular polymeric substances (EPS) of thermoacidophilic archaeon Acidianus manzaensis YN-25 acclimated with different energy substrates (FeS2, CuFeS2, S0, FeSO4).
30179696	3	84	from	substrates	746:755	arg1	composition					784:794	organic composition	776:794	organic composition in terms of typical function groups (CO, CO and CN)	776:846	XANES results showed clear associations between the energy substrates and the changes in organic composition in terms of typical function groups (CO, CO and CN).
30179696	4	85	contain	contained	895:903	arg2	proportion					912:921	higher proportion	905:921	higher proportion of proteins	905:933	The chalcopyrite- and pyrite-acclimated cells contained higher proportion of proteins but less proportion of polysaccharides than the S0-acclimated cells.
30179696	4	85	contain	contained	895:903	arg1	cells					889:893	The chalcopyrite- and pyrite-acclimated cells	849:893	The chalcopyrite- and pyrite-acclimated cells	849:893	The chalcopyrite- and pyrite-acclimated cells contained higher proportion of proteins but less proportion of polysaccharides than the S0-acclimated cells.
30179696	0	86	theme	organic	50:56	arg1	components					58:67	superficial organic components	38:67	superficial organic components of thermoacidophilic archaeon Acidianus manzaensis YN-25	38:124	In situ characterization of change in superficial organic components of thermoacidophilic archaeon Acidianus manzaensis YN-25.
30179696	5	87	theme	S0-acclimated	1087:1099	arg1	cells					1101:1105	the S0-acclimated cells	1083:1105	the S0-acclimated cells	1083:1105	The FeSO4-acclimated cells contained the highest proportion of proteins, while the S0-acclimated cells contained more lipids and polysaccharides.
30179696	3	88	from	composition	784:794	arg1	terms					799:803	terms	799:803	terms of typical function groups (CO, CO and CN)	799:846	XANES results showed clear associations between the energy substrates and the changes in organic composition in terms of typical function groups (CO, CO and CN).
30179696	1	89	theme	superficial	321:331	arg1	components					355:364	superficial (about 10 nm) organic components	321:364	superficial (about 10 nm) organic components	321:364	For the first time, synchrotron radiation (SR) -based carbon K-edge X-ray absorption near edge structure (XANES) spectroscopy in-situ characterization was conducted to evaluate the evolution of superficial (about 10 nm) organic components of extracellular polymeric substances (EPS) of thermoacidophilic archaeon Acidianus manzaensis YN-25 acclimated with different energy substrates (FeS2, CuFeS2, S0, FeSO4).
30179696	7	90	theme	characterization	1540:1555	arg1	methods					1557:1563	the latter two characterization methods	1525:1563	the latter two characterization methods	1525:1563	These differences are caused by the inconsistencies of measurement depth between XANES and the latter two characterization methods.
30179696	1	91	theme	structure	222:230	arg1	spectroscopy					240:251	synchrotron radiation (SR) -based carbon K-edge X-ray absorption near edge structure (XANES) spectroscopy	147:251	synchrotron radiation (SR) -based carbon K-edge X-ray absorption near edge structure (XANES) spectroscopy in-situ characterization	147:276	For the first time, synchrotron radiation (SR) -based carbon K-edge X-ray absorption near edge structure (XANES) spectroscopy in-situ characterization was conducted to evaluate the evolution of superficial (about 10 nm) organic components of extracellular polymeric substances (EPS) of thermoacidophilic archaeon Acidianus manzaensis YN-25 acclimated with different energy substrates (FeS2, CuFeS2, S0, FeSO4).
30179696	7	92	theme	latter	1529:1534	arg1	methods					1557:1563	the latter two characterization methods	1525:1563	the latter two characterization methods	1525:1563	These differences are caused by the inconsistencies of measurement depth between XANES and the latter two characterization methods.
30179696	6	93	from	trend	1298:1302	arg1	comparison					1307:1316	comparison	1307:1316	comparison with the results of FTIR and spectrophotometric determination	1307:1378	The results of linear-combination and peak fitting of the K-edge XANES for the extracellular superficial organic component C is consistent with the trend in comparison with the results of FTIR and spectrophotometric determination, but there are significant differences in the values.
30179696	1	94	theme	XANES	233:237	arg1	spectroscopy					240:251	synchrotron radiation (SR) -based carbon K-edge X-ray absorption near edge structure (XANES) spectroscopy	147:251	synchrotron radiation (SR) -based carbon K-edge X-ray absorption near edge structure (XANES) spectroscopy in-situ characterization	147:276	For the first time, synchrotron radiation (SR) -based carbon K-edge X-ray absorption near edge structure (XANES) spectroscopy in-situ characterization was conducted to evaluate the evolution of superficial (about 10 nm) organic components of extracellular polymeric substances (EPS) of thermoacidophilic archaeon Acidianus manzaensis YN-25 acclimated with different energy substrates (FeS2, CuFeS2, S0, FeSO4).
31787665	6	0	theme	membrane	886:893	arg1	fraction					895:902	the heavy membrane fraction	876:902	the heavy membrane fraction	876:902	The mutant Rim21, but not WT Rim21, was mainly fractionated into the heavy membrane fraction.
31787665	8	1	theme	biochemical	1087:1097	arg1	properties					1099:1108	these different biochemical properties	1071:1108	these different biochemical properties from WT Rim21	1071:1122	Despite these different biochemical properties from WT Rim21, mutant Rim21 protein could still activate the Rim101 pathway in response to external alkalization.
31787665	8	2	theme	different	1077:1085	arg1	properties					1099:1108	these different biochemical properties	1071:1108	these different biochemical properties from WT Rim21	1071:1122	Despite these different biochemical properties from WT Rim21, mutant Rim21 protein could still activate the Rim101 pathway in response to external alkalization.
31787665	6	3	theme	heavy	880:884	arg1	fraction					895:902	the heavy membrane fraction	876:902	the heavy membrane fraction	876:902	The mutant Rim21, but not WT Rim21, was mainly fractionated into the heavy membrane fraction.
31787665	9	4	theme	lipid	1429:1433	arg1	conditions					1435:1444	distinct lipid conditions	1420:1444	distinct lipid conditions	1420:1444	Collectively, N-glycosylation of Rim21 is not indispensable for its activity as a sensor protein, but modulates the residence of Rim21 protein to some microdomains within the plasma membrane with distinct lipid conditions, thereby affecting its turnover.Key words: plasma membrane, lipid asymmetry, N-linked glycosylation, microdomain, Saccharomyces cerevisiae.
31787665	3	5	theme	unconventional	473:486	arg1	motif					488:492	an unconventional motif	470:492	an unconventional motif located in the N-terminal extracellular region	470:539	Using a systematic mutation analysis, we found that Rim21 is N-glycosylated at an unconventional motif located in the N-terminal extracellular region.
31787665	6	6	theme	WT	837:838	arg1	Rim21					840:844	WT Rim21	837:844	WT Rim21	837:844	The mutant Rim21, but not WT Rim21, was mainly fractionated into the heavy membrane fraction.
31787665	1	7	theme	Rim101	241:246	arg1	pathway					248:254	the Rim101 pathway	237:254	the Rim101 pathway in budding yeast	237:271	The polytopic plasma membrane protein Rim21 senses both the elevation of ambient pH and alterations in plasma membrane lipid asymmetry in the Rim101 pathway in budding yeast.
31787665	5	8	theme	mutant	703:708	arg1	Rim21					710:714	both the WT and mutant Rim21	687:714	Rim21	710:714	Although both the WT and mutant Rim21 localized to the plasma membrane, they exhibited different biochemical fractionation profiles.
31787665	3	9	theme	N-terminal	509:518	arg1	region					534:539	the N-terminal extracellular region	505:539	the N-terminal extracellular region	505:539	Using a systematic mutation analysis, we found that Rim21 is N-glycosylated at an unconventional motif located in the N-terminal extracellular region.
31787665	0	10	theme	Plasma	82:87	arg1	Membrane					89:96	the Plasma Membrane	78:96	the Plasma Membrane	78:96	N-glycosylation of Rim21 at an Unconventional Site Fine-tunes Its Behavior in the Plasma Membrane.
31787665	3	11	theme	extracellular	520:532	arg1	region					534:539	the N-terminal extracellular region	505:539	the N-terminal extracellular region	505:539	Using a systematic mutation analysis, we found that Rim21 is N-glycosylated at an unconventional motif located in the N-terminal extracellular region.
31787665	3	12	from	motif	488:492	arg1	N-glycosylated					452:465	N-glycosylated	452:465	N-glycosylated	452:465	Using a systematic mutation analysis, we found that Rim21 is N-glycosylated at an unconventional motif located in the N-terminal extracellular region.
31787665	2	13	dep	site	325:328	arg1	the					321:323	the	321:323	the	321:323	Rim21 is known to undergo N-glycosylation, but the site and function of the glycosylation modification is not known.
31787665	9	14	link	N-linked	1523:1530	arg1	membrane					1496:1503	plasma membrane	1489:1503	plasma membrane	1489:1503	Collectively, N-glycosylation of Rim21 is not indispensable for its activity as a sensor protein, but modulates the residence of Rim21 protein to some microdomains within the plasma membrane with distinct lipid conditions, thereby affecting its turnover.Key words: plasma membrane, lipid asymmetry, N-linked glycosylation, microdomain, Saccharomyces cerevisiae.
31787665	9	14	link	N-linked	1523:1530	arg1	glycosylation					1532:1544	N-linked glycosylation	1523:1544	N-linked glycosylation	1523:1544	Collectively, N-glycosylation of Rim21 is not indispensable for its activity as a sensor protein, but modulates the residence of Rim21 protein to some microdomains within the plasma membrane with distinct lipid conditions, thereby affecting its turnover.Key words: plasma membrane, lipid asymmetry, N-linked glycosylation, microdomain, Saccharomyces cerevisiae.
31787665	0	15	from	Site	46:49	arg1	N-glycosylation					0:14	N-glycosylation	0:14	N-glycosylation of Rim21 at an Unconventional Site	0:49	N-glycosylation of Rim21 at an Unconventional Site Fine-tunes Its Behavior in the Plasma Membrane.
31787665	3	16	from	located	494:500	arg1	region					534:539	the N-terminal extracellular region	505:539	the N-terminal extracellular region	505:539	Using a systematic mutation analysis, we found that Rim21 is N-glycosylated at an unconventional motif located in the N-terminal extracellular region.
31787665	9	17	theme	N-linked	1523:1530	arg1	membrane					1496:1503	plasma membrane	1489:1503	plasma membrane	1489:1503	Collectively, N-glycosylation of Rim21 is not indispensable for its activity as a sensor protein, but modulates the residence of Rim21 protein to some microdomains within the plasma membrane with distinct lipid conditions, thereby affecting its turnover.Key words: plasma membrane, lipid asymmetry, N-linked glycosylation, microdomain, Saccharomyces cerevisiae.
31787665	9	17	theme	N-linked	1523:1530	arg1	glycosylation					1532:1544	N-linked glycosylation	1523:1544	N-linked glycosylation	1523:1544	Collectively, N-glycosylation of Rim21 is not indispensable for its activity as a sensor protein, but modulates the residence of Rim21 protein to some microdomains within the plasma membrane with distinct lipid conditions, thereby affecting its turnover.Key words: plasma membrane, lipid asymmetry, N-linked glycosylation, microdomain, Saccharomyces cerevisiae.
31787665	1	18	theme	ambient	172:178	arg1	pH					180:181	ambient pH	172:181	ambient pH	172:181	The polytopic plasma membrane protein Rim21 senses both the elevation of ambient pH and alterations in plasma membrane lipid asymmetry in the Rim101 pathway in budding yeast.
31787665	3	19	theme	mutation	410:417	arg1	analysis					419:426	a systematic mutation analysis	397:426	a systematic mutation analysis	397:426	Using a systematic mutation analysis, we found that Rim21 is N-glycosylated at an unconventional motif located in the N-terminal extracellular region.
31787665	9	20	theme	plasma	1399:1404	arg1	membrane					1406:1413	the plasma membrane	1395:1413	the plasma membrane with distinct lipid conditions	1395:1444	Collectively, N-glycosylation of Rim21 is not indispensable for its activity as a sensor protein, but modulates the residence of Rim21 protein to some microdomains within the plasma membrane with distinct lipid conditions, thereby affecting its turnover.Key words: plasma membrane, lipid asymmetry, N-linked glycosylation, microdomain, Saccharomyces cerevisiae.
31787665	9	21	theme	protein	1359:1365	arg1	residence					1340:1348	the residence	1336:1348	the residence of Rim21 protein to some microdomains within the plasma membrane with distinct lipid conditions	1336:1444	Collectively, N-glycosylation of Rim21 is not indispensable for its activity as a sensor protein, but modulates the residence of Rim21 protein to some microdomains within the plasma membrane with distinct lipid conditions, thereby affecting its turnover.Key words: plasma membrane, lipid asymmetry, N-linked glycosylation, microdomain, Saccharomyces cerevisiae.
31787665	2	22	theme	modification	364:375	arg1	modification					364:375	the glycosylation modification	346:375	the glycosylation modification	346:375	Rim21 is known to undergo N-glycosylation, but the site and function of the glycosylation modification is not known.
31787665	2	22	theme	modification	364:375	arg1	function					334:341	function	334:341	function	334:341	Rim21 is known to undergo N-glycosylation, but the site and function of the glycosylation modification is not known.
31787665	2	22	theme	modification	364:375	arg1	site					325:328	site	325:328	site	325:328	Rim21 is known to undergo N-glycosylation, but the site and function of the glycosylation modification is not known.
31787665	5	23	theme	fractionation	787:799	arg1	profiles					801:808	different biochemical fractionation profiles	765:808	different biochemical fractionation profiles	765:808	Although both the WT and mutant Rim21 localized to the plasma membrane, they exhibited different biochemical fractionation profiles.
31787665	7	24	theme	Triton	1049:1054	arg1	X-100					1056:1060	Triton X-100	1049:1060	Triton X-100	1049:1060	Further, compared to WT Rim21, mutant Rim21 was more easily solubilized with digitonin but was conversely more resistant to solubilization with Triton X-100.
31787665	9	25	theme	sensor	1306:1311	arg1	protein					1313:1319	a sensor protein	1304:1319	a sensor protein	1304:1319	Collectively, N-glycosylation of Rim21 is not indispensable for its activity as a sensor protein, but modulates the residence of Rim21 protein to some microdomains within the plasma membrane with distinct lipid conditions, thereby affecting its turnover.Key words: plasma membrane, lipid asymmetry, N-linked glycosylation, microdomain, Saccharomyces cerevisiae.
31787665	2	26	theme	glycosylation	350:362	arg1	modification					364:375	the glycosylation modification	346:375	the glycosylation modification	346:375	Rim21 is known to undergo N-glycosylation, but the site and function of the glycosylation modification is not known.
31787665	1	27	theme	budding	259:265	arg1	yeast					267:271	budding yeast	259:271	budding yeast	259:271	The polytopic plasma membrane protein Rim21 senses both the elevation of ambient pH and alterations in plasma membrane lipid asymmetry in the Rim101 pathway in budding yeast.
31787665	9	28	theme	Rim21	1257:1261	arg1	N-glycosylation					1238:1252	N-glycosylation	1238:1252	N-glycosylation of Rim21	1238:1261	Collectively, N-glycosylation of Rim21 is not indispensable for its activity as a sensor protein, but modulates the residence of Rim21 protein to some microdomains within the plasma membrane with distinct lipid conditions, thereby affecting its turnover.Key words: plasma membrane, lipid asymmetry, N-linked glycosylation, microdomain, Saccharomyces cerevisiae.
31787665	3	29	gly	N-glycosylated	452:465	arg1	Rim21					443:447	Rim21	443:447	Rim21	443:447	Using a systematic mutation analysis, we found that Rim21 is N-glycosylated at an unconventional motif located in the N-terminal extracellular region.
31787665	3	29	gly	N-glycosylated	452:465	arg2	motif					488:492	an unconventional motif	470:492	an unconventional motif located in the N-terminal extracellular region	470:539	Using a systematic mutation analysis, we found that Rim21 is N-glycosylated at an unconventional motif located in the N-terminal extracellular region.
31787665	1	30	theme	pH	180:181	arg1	alterations					187:197	alterations	187:197	alterations in plasma membrane lipid asymmetry in the Rim101 pathway in budding yeast	187:271	The polytopic plasma membrane protein Rim21 senses both the elevation of ambient pH and alterations in plasma membrane lipid asymmetry in the Rim101 pathway in budding yeast.
31787665	1	30	theme	pH	180:181	arg1	elevation					159:167	the elevation	155:167	the elevation of ambient pH	155:181	The polytopic plasma membrane protein Rim21 senses both the elevation of ambient pH and alterations in plasma membrane lipid asymmetry in the Rim101 pathway in budding yeast.
31787665	8	31	theme	Rim101	1171:1176	arg1	pathway					1178:1184	the Rim101 pathway	1167:1184	the Rim101 pathway	1167:1184	Despite these different biochemical properties from WT Rim21, mutant Rim21 protein could still activate the Rim101 pathway in response to external alkalization.
31787665	1	32	from	pathway	248:254	arg1	yeast					267:271	budding yeast	259:271	budding yeast	259:271	The polytopic plasma membrane protein Rim21 senses both the elevation of ambient pH and alterations in plasma membrane lipid asymmetry in the Rim101 pathway in budding yeast.
31787665	7	33	theme	WT	926:927	arg1	Rim21					929:933	WT Rim21	926:933	WT Rim21	926:933	Further, compared to WT Rim21, mutant Rim21 was more easily solubilized with digitonin but was conversely more resistant to solubilization with Triton X-100.
31787665	8	34	dep	alkalization	1210:1221	arg1	response					1189:1196	response	1189:1196	response	1189:1196	Despite these different biochemical properties from WT Rim21, mutant Rim21 protein could still activate the Rim101 pathway in response to external alkalization.
31787665	4	35	theme	protein	623:629	arg1	lifetime					631:638	prolonged protein lifetime	613:638	prolonged protein lifetime	613:638	The Rim21 mutant protein that failed to receive N-glycosylation showed prolonged protein lifetime compared to that of WT Rim21 protein.
31787665	0	36	theme	Rim21	19:23	arg1	N-glycosylation					0:14	N-glycosylation	0:14	N-glycosylation of Rim21 at an Unconventional Site	0:49	N-glycosylation of Rim21 at an Unconventional Site Fine-tunes Its Behavior in the Plasma Membrane.
31787665	4	37	theme	Rim21	546:550	arg1	protein					559:565	The Rim21 mutant protein	542:565	The Rim21 mutant protein that failed to receive N-glycosylation	542:604	The Rim21 mutant protein that failed to receive N-glycosylation showed prolonged protein lifetime compared to that of WT Rim21 protein.
31787665	9	38	theme	lipid	1506:1510	arg1	membrane					1496:1503	plasma membrane	1489:1503	plasma membrane	1489:1503	Collectively, N-glycosylation of Rim21 is not indispensable for its activity as a sensor protein, but modulates the residence of Rim21 protein to some microdomains within the plasma membrane with distinct lipid conditions, thereby affecting its turnover.Key words: plasma membrane, lipid asymmetry, N-linked glycosylation, microdomain, Saccharomyces cerevisiae.
31787665	9	38	theme	lipid	1506:1510	arg1	asymmetry					1512:1520	lipid asymmetry	1506:1520	lipid asymmetry	1506:1520	Collectively, N-glycosylation of Rim21 is not indispensable for its activity as a sensor protein, but modulates the residence of Rim21 protein to some microdomains within the plasma membrane with distinct lipid conditions, thereby affecting its turnover.Key words: plasma membrane, lipid asymmetry, N-linked glycosylation, microdomain, Saccharomyces cerevisiae.
31787665	4	39	theme	prolonged	613:621	arg1	lifetime					631:638	prolonged protein lifetime	613:638	prolonged protein lifetime	613:638	The Rim21 mutant protein that failed to receive N-glycosylation showed prolonged protein lifetime compared to that of WT Rim21 protein.
31787665	0	40	theme	Unconventional	31:44	arg1	Site					46:49	an Unconventional Site	28:49	an Unconventional Site	28:49	N-glycosylation of Rim21 at an Unconventional Site Fine-tunes Its Behavior in the Plasma Membrane.
31787665	4	41	theme	WT	660:661	arg1	protein					669:675	WT Rim21 protein	660:675	WT Rim21 protein	660:675	The Rim21 mutant protein that failed to receive N-glycosylation showed prolonged protein lifetime compared to that of WT Rim21 protein.
31787665	5	42	theme	WT	696:697	arg1	Rim21					710:714	both the WT and mutant Rim21	687:714	Rim21	710:714	Although both the WT and mutant Rim21 localized to the plasma membrane, they exhibited different biochemical fractionation profiles.
31787665	8	43	from	Rim21	1118:1122	arg1	properties					1099:1108	these different biochemical properties	1071:1108	these different biochemical properties from WT Rim21	1071:1122	Despite these different biochemical properties from WT Rim21, mutant Rim21 protein could still activate the Rim101 pathway in response to external alkalization.
31787665	0	44	gly	N-glycosylation	0:14	arg1	Rim21					19:23	Rim21	19:23	Rim21	19:23	N-glycosylation of Rim21 at an Unconventional Site Fine-tunes Its Behavior in the Plasma Membrane.
31787665	0	44	gly	N-glycosylation	0:14	arg2	Site					46:49	an Unconventional Site	28:49	an Unconventional Site	28:49	N-glycosylation of Rim21 at an Unconventional Site Fine-tunes Its Behavior in the Plasma Membrane.
31787665	0	44	gly	N-glycosylation	0:14	arg1	Site					46:49	an Unconventional Site	28:49	an Unconventional Site	28:49	N-glycosylation of Rim21 at an Unconventional Site Fine-tunes Its Behavior in the Plasma Membrane.
31787665	3	45	theme	systematic	399:408	arg1	analysis					419:426	a systematic mutation analysis	397:426	a systematic mutation analysis	397:426	Using a systematic mutation analysis, we found that Rim21 is N-glycosylated at an unconventional motif located in the N-terminal extracellular region.
31787665	7	46	with	solubilization	1029:1042	arg1	X-100					1056:1060	Triton X-100	1049:1060	Triton X-100	1049:1060	Further, compared to WT Rim21, mutant Rim21 was more easily solubilized with digitonin but was conversely more resistant to solubilization with Triton X-100.
31787665	9	47	theme	Rim21	1353:1357	arg1	protein					1359:1365	Rim21 protein	1353:1365	Rim21 protein	1353:1365	Collectively, N-glycosylation of Rim21 is not indispensable for its activity as a sensor protein, but modulates the residence of Rim21 protein to some microdomains within the plasma membrane with distinct lipid conditions, thereby affecting its turnover.Key words: plasma membrane, lipid asymmetry, N-linked glycosylation, microdomain, Saccharomyces cerevisiae.
31787665	4	48	theme	mutant	552:557	arg1	protein					559:565	The Rim21 mutant protein	542:565	The Rim21 mutant protein that failed to receive N-glycosylation	542:604	The Rim21 mutant protein that failed to receive N-glycosylation showed prolonged protein lifetime compared to that of WT Rim21 protein.
31787665	0	49	from	Behavior	66:73	arg1	Membrane					89:96	the Plasma Membrane	78:96	the Plasma Membrane	78:96	N-glycosylation of Rim21 at an Unconventional Site Fine-tunes Its Behavior in the Plasma Membrane.
31787665	1	50	theme	plasma	202:207	arg1	asymmetry					224:232	plasma membrane lipid asymmetry	202:232	plasma membrane lipid asymmetry in the Rim101 pathway in budding yeast	202:271	The polytopic plasma membrane protein Rim21 senses both the elevation of ambient pH and alterations in plasma membrane lipid asymmetry in the Rim101 pathway in budding yeast.
31787665	5	51	theme	different	765:773	arg1	profiles					801:808	different biochemical fractionation profiles	765:808	different biochemical fractionation profiles	765:808	Although both the WT and mutant Rim21 localized to the plasma membrane, they exhibited different biochemical fractionation profiles.
31787665	8	52	theme	Rim21	1132:1136	arg1	protein					1138:1144	mutant Rim21 protein	1125:1144	mutant Rim21 protein	1125:1144	Despite these different biochemical properties from WT Rim21, mutant Rim21 protein could still activate the Rim101 pathway in response to external alkalization.
31787665	1	53	from	asymmetry	224:232	arg1	pathway					248:254	the Rim101 pathway	237:254	the Rim101 pathway in budding yeast	237:271	The polytopic plasma membrane protein Rim21 senses both the elevation of ambient pH and alterations in plasma membrane lipid asymmetry in the Rim101 pathway in budding yeast.
31787665	9	54	theme	turnover.Key	1469:1480	arg1	words					1482:1486	its turnover.Key words	1465:1486	its turnover.Key words: plasma membrane, lipid asymmetry, N-linked glycosylation, microdomain, Saccharomyces cerevisiae	1465:1583	Collectively, N-glycosylation of Rim21 is not indispensable for its activity as a sensor protein, but modulates the residence of Rim21 protein to some microdomains within the plasma membrane with distinct lipid conditions, thereby affecting its turnover.Key words: plasma membrane, lipid asymmetry, N-linked glycosylation, microdomain, Saccharomyces cerevisiae.
31787665	9	55	with	membrane	1406:1413	arg1	conditions					1435:1444	distinct lipid conditions	1420:1444	distinct lipid conditions	1420:1444	Collectively, N-glycosylation of Rim21 is not indispensable for its activity as a sensor protein, but modulates the residence of Rim21 protein to some microdomains within the plasma membrane with distinct lipid conditions, thereby affecting its turnover.Key words: plasma membrane, lipid asymmetry, N-linked glycosylation, microdomain, Saccharomyces cerevisiae.
31787665	1	56	theme	polytopic	103:111	arg1	protein					129:135	The polytopic plasma membrane protein Rim21	99:141	The polytopic plasma membrane protein Rim21	99:141	The polytopic plasma membrane protein Rim21 senses both the elevation of ambient pH and alterations in plasma membrane lipid asymmetry in the Rim101 pathway in budding yeast.
31787665	5	57	theme	biochemical	775:785	arg1	profiles					801:808	different biochemical fractionation profiles	765:808	different biochemical fractionation profiles	765:808	Although both the WT and mutant Rim21 localized to the plasma membrane, they exhibited different biochemical fractionation profiles.
31787665	1	58	theme	plasma	113:118	arg1	protein					129:135	The polytopic plasma membrane protein Rim21	99:141	The polytopic plasma membrane protein Rim21	99:141	The polytopic plasma membrane protein Rim21 senses both the elevation of ambient pH and alterations in plasma membrane lipid asymmetry in the Rim101 pathway in budding yeast.
31787665	3	59	from	region	534:539	arg1	located					494:500	located	494:500	located	494:500	Using a systematic mutation analysis, we found that Rim21 is N-glycosylated at an unconventional motif located in the N-terminal extracellular region.
31787665	2	60	gly	N-glycosylation	300:314	arg1	Rim21					274:278	Rim21	274:278	Rim21	274:278	Rim21 is known to undergo N-glycosylation, but the site and function of the glycosylation modification is not known.
31787665	1	61	from	alterations	187:197	arg1	asymmetry					224:232	plasma membrane lipid asymmetry	202:232	plasma membrane lipid asymmetry in the Rim101 pathway in budding yeast	202:271	The polytopic plasma membrane protein Rim21 senses both the elevation of ambient pH and alterations in plasma membrane lipid asymmetry in the Rim101 pathway in budding yeast.
31787665	1	62	theme	membrane	120:127	arg1	protein					129:135	The polytopic plasma membrane protein Rim21	99:141	The polytopic plasma membrane protein Rim21	99:141	The polytopic plasma membrane protein Rim21 senses both the elevation of ambient pH and alterations in plasma membrane lipid asymmetry in the Rim101 pathway in budding yeast.
31787665	7	63	theme	mutant	936:941	arg1	Rim21					943:947	mutant Rim21	936:947	mutant Rim21	936:947	Further, compared to WT Rim21, mutant Rim21 was more easily solubilized with digitonin but was conversely more resistant to solubilization with Triton X-100.
31787665	1	64	theme	membrane	209:216	arg1	asymmetry					224:232	plasma membrane lipid asymmetry	202:232	plasma membrane lipid asymmetry in the Rim101 pathway in budding yeast	202:271	The polytopic plasma membrane protein Rim21 senses both the elevation of ambient pH and alterations in plasma membrane lipid asymmetry in the Rim101 pathway in budding yeast.
31787665	5	65	dep	Rim21	710:714	arg1	both					687:690	both	687:690	both	687:690	Although both the WT and mutant Rim21 localized to the plasma membrane, they exhibited different biochemical fractionation profiles.
31787665	9	66	dep	words	1482:1486	arg1	membrane					1496:1503	plasma membrane	1489:1503	plasma membrane	1489:1503	Collectively, N-glycosylation of Rim21 is not indispensable for its activity as a sensor protein, but modulates the residence of Rim21 protein to some microdomains within the plasma membrane with distinct lipid conditions, thereby affecting its turnover.Key words: plasma membrane, lipid asymmetry, N-linked glycosylation, microdomain, Saccharomyces cerevisiae.
31787665	9	66	dep	words	1482:1486	arg1	cerevisiae					1574:1583	Saccharomyces cerevisiae	1560:1583	Saccharomyces cerevisiae	1560:1583	Collectively, N-glycosylation of Rim21 is not indispensable for its activity as a sensor protein, but modulates the residence of Rim21 protein to some microdomains within the plasma membrane with distinct lipid conditions, thereby affecting its turnover.Key words: plasma membrane, lipid asymmetry, N-linked glycosylation, microdomain, Saccharomyces cerevisiae.
31787665	9	66	dep	words	1482:1486	arg1	glycosylation					1532:1544	N-linked glycosylation	1523:1544	N-linked glycosylation	1523:1544	Collectively, N-glycosylation of Rim21 is not indispensable for its activity as a sensor protein, but modulates the residence of Rim21 protein to some microdomains within the plasma membrane with distinct lipid conditions, thereby affecting its turnover.Key words: plasma membrane, lipid asymmetry, N-linked glycosylation, microdomain, Saccharomyces cerevisiae.
31787665	9	66	dep	words	1482:1486	arg1	asymmetry					1512:1520	lipid asymmetry	1506:1520	lipid asymmetry	1506:1520	Collectively, N-glycosylation of Rim21 is not indispensable for its activity as a sensor protein, but modulates the residence of Rim21 protein to some microdomains within the plasma membrane with distinct lipid conditions, thereby affecting its turnover.Key words: plasma membrane, lipid asymmetry, N-linked glycosylation, microdomain, Saccharomyces cerevisiae.
31787665	9	66	dep	words	1482:1486	arg1	microdomain					1547:1557	microdomain	1547:1557	microdomain	1547:1557	Collectively, N-glycosylation of Rim21 is not indispensable for its activity as a sensor protein, but modulates the residence of Rim21 protein to some microdomains within the plasma membrane with distinct lipid conditions, thereby affecting its turnover.Key words: plasma membrane, lipid asymmetry, N-linked glycosylation, microdomain, Saccharomyces cerevisiae.
31787665	4	67	theme	Rim21	663:667	arg1	protein					669:675	WT Rim21 protein	660:675	WT Rim21 protein	660:675	The Rim21 mutant protein that failed to receive N-glycosylation showed prolonged protein lifetime compared to that of WT Rim21 protein.
31787665	1	68	theme	lipid	218:222	arg1	asymmetry					224:232	plasma membrane lipid asymmetry	202:232	plasma membrane lipid asymmetry in the Rim101 pathway in budding yeast	202:271	The polytopic plasma membrane protein Rim21 senses both the elevation of ambient pH and alterations in plasma membrane lipid asymmetry in the Rim101 pathway in budding yeast.
31787665	8	69	theme	WT	1115:1116	arg1	Rim21					1118:1122	WT Rim21	1115:1122	WT Rim21	1115:1122	Despite these different biochemical properties from WT Rim21, mutant Rim21 protein could still activate the Rim101 pathway in response to external alkalization.
31787665	6	70	theme	mutant	815:820	arg1	Rim21					822:826	The mutant Rim21	811:826	The mutant Rim21	811:826	The mutant Rim21, but not WT Rim21, was mainly fractionated into the heavy membrane fraction.
31787665	9	71	gly	N-glycosylation	1238:1252	arg1	Rim21					1257:1261	Rim21	1257:1261	Rim21	1257:1261	Collectively, N-glycosylation of Rim21 is not indispensable for its activity as a sensor protein, but modulates the residence of Rim21 protein to some microdomains within the plasma membrane with distinct lipid conditions, thereby affecting its turnover.Key words: plasma membrane, lipid asymmetry, N-linked glycosylation, microdomain, Saccharomyces cerevisiae.
31787665	9	72	theme	plasma	1489:1494	arg1	membrane					1496:1503	plasma membrane	1489:1503	plasma membrane	1489:1503	Collectively, N-glycosylation of Rim21 is not indispensable for its activity as a sensor protein, but modulates the residence of Rim21 protein to some microdomains within the plasma membrane with distinct lipid conditions, thereby affecting its turnover.Key words: plasma membrane, lipid asymmetry, N-linked glycosylation, microdomain, Saccharomyces cerevisiae.
31787665	9	72	theme	plasma	1489:1494	arg1	cerevisiae					1574:1583	Saccharomyces cerevisiae	1560:1583	Saccharomyces cerevisiae	1560:1583	Collectively, N-glycosylation of Rim21 is not indispensable for its activity as a sensor protein, but modulates the residence of Rim21 protein to some microdomains within the plasma membrane with distinct lipid conditions, thereby affecting its turnover.Key words: plasma membrane, lipid asymmetry, N-linked glycosylation, microdomain, Saccharomyces cerevisiae.
31787665	9	72	theme	plasma	1489:1494	arg1	glycosylation					1532:1544	N-linked glycosylation	1523:1544	N-linked glycosylation	1523:1544	Collectively, N-glycosylation of Rim21 is not indispensable for its activity as a sensor protein, but modulates the residence of Rim21 protein to some microdomains within the plasma membrane with distinct lipid conditions, thereby affecting its turnover.Key words: plasma membrane, lipid asymmetry, N-linked glycosylation, microdomain, Saccharomyces cerevisiae.
31787665	9	72	theme	plasma	1489:1494	arg1	asymmetry					1512:1520	lipid asymmetry	1506:1520	lipid asymmetry	1506:1520	Collectively, N-glycosylation of Rim21 is not indispensable for its activity as a sensor protein, but modulates the residence of Rim21 protein to some microdomains within the plasma membrane with distinct lipid conditions, thereby affecting its turnover.Key words: plasma membrane, lipid asymmetry, N-linked glycosylation, microdomain, Saccharomyces cerevisiae.
31787665	9	72	theme	plasma	1489:1494	arg1	microdomain					1547:1557	microdomain	1547:1557	microdomain	1547:1557	Collectively, N-glycosylation of Rim21 is not indispensable for its activity as a sensor protein, but modulates the residence of Rim21 protein to some microdomains within the plasma membrane with distinct lipid conditions, thereby affecting its turnover.Key words: plasma membrane, lipid asymmetry, N-linked glycosylation, microdomain, Saccharomyces cerevisiae.
31787665	8	73	theme	external	1201:1208	arg1	alkalization					1210:1221	external alkalization	1201:1221	external alkalization	1201:1221	Despite these different biochemical properties from WT Rim21, mutant Rim21 protein could still activate the Rim101 pathway in response to external alkalization.
31787665	5	74	theme	plasma	733:738	arg1	membrane					740:747	the plasma membrane	729:747	the plasma membrane	729:747	Although both the WT and mutant Rim21 localized to the plasma membrane, they exhibited different biochemical fractionation profiles.
31787665	9	75	theme	distinct	1420:1427	arg1	conditions					1435:1444	distinct lipid conditions	1420:1444	distinct lipid conditions	1420:1444	Collectively, N-glycosylation of Rim21 is not indispensable for its activity as a sensor protein, but modulates the residence of Rim21 protein to some microdomains within the plasma membrane with distinct lipid conditions, thereby affecting its turnover.Key words: plasma membrane, lipid asymmetry, N-linked glycosylation, microdomain, Saccharomyces cerevisiae.
31787665	1	76	from	elevation	159:167	arg1	asymmetry					224:232	plasma membrane lipid asymmetry	202:232	plasma membrane lipid asymmetry in the Rim101 pathway in budding yeast	202:271	The polytopic plasma membrane protein Rim21 senses both the elevation of ambient pH and alterations in plasma membrane lipid asymmetry in the Rim101 pathway in budding yeast.
31787665	3	77	theme	located	494:500	arg1	motif					488:492	an unconventional motif	470:492	an unconventional motif located in the N-terminal extracellular region	470:539	Using a systematic mutation analysis, we found that Rim21 is N-glycosylated at an unconventional motif located in the N-terminal extracellular region.
31787665	8	78	theme	mutant	1125:1130	arg1	protein					1138:1144	mutant Rim21 protein	1125:1144	mutant Rim21 protein	1125:1144	Despite these different biochemical properties from WT Rim21, mutant Rim21 protein could still activate the Rim101 pathway in response to external alkalization.
31430995	2	0	theme	cell	312:315	arg1	maturation					317:326	cell maturation	312:326	cell maturation	312:326	Since these three heights correspond to different states in stem development, a view on the dynamics of the cell wall proteome during cell maturation is obtained.
31430995	3	1	theme	localized	496:504	arg1	proteins					506:513	a description of the development process of the cell wall and the linking cell wall localized proteins	412:513	a description of the development process of the cell wall and the linking cell wall localized proteins with the evolution of cell wall composition and structure	412:571	This study of cell wall protein-enriched fractions forms the basis for a description of the development process of the cell wall and the linking cell wall localized proteins with the evolution of cell wall composition and structure.
31430995	4	2	theme	proteins	613:620	arg1	extraction					589:598	The sequential extraction	574:598	The sequential extraction of cell wall proteins with CaCl2, EGTA, and LiCl-complemented buffers	574:668	The sequential extraction of cell wall proteins with CaCl2, EGTA, and LiCl-complemented buffers was combined with a gel-based proteome approach and multivariate analysis.
31430995	3	3	theme	cell	486:489	arg1	wall					491:494	the linking cell wall	474:494	the linking cell wall	474:494	This study of cell wall protein-enriched fractions forms the basis for a description of the development process of the cell wall and the linking cell wall localized proteins with the evolution of cell wall composition and structure.
31430995	1	4	theme	stems	157:161	arg1	heights					138:144	three different heights	122:144	three different heights of alfalfa stems	122:161	In this study, the cell-wall-enriched subproteomes at three different heights of alfalfa stems were compared.
31430995	8	5	theme	nutrients	1163:1171	arg1	process					1200:1206	an important process	1187:1206	an important process	1187:1206	Therefore, remobilization of nutrients appears to be an important process in the oldest stem segment.
31430995	8	5	theme	nutrients	1163:1171	arg1	remobilization					1145:1158	remobilization	1145:1158	remobilization of nutrients	1145:1171	Therefore, remobilization of nutrients appears to be an important process in the oldest stem segment.
31430995	10	6	contain	containing	1657:1666	arg2	protein					1668:1674	protein	1668:1674	protein	1668:1674	However, the most striking change between the different stem parts is the strong accumulation of a DUF642-conserved domain containing protein in the apical region of the stem, which suggests a particular role of this protein during the early development of stem tissues.
31430995	10	6	contain	containing	1657:1666	arg1	domain					1650:1655	a DUF642-conserved domain	1631:1655	a DUF642-conserved domain containing protein in the apical region of the stem, which suggests a particular role of this protein during the early development of stem tissues	1631:1802	However, the most striking change between the different stem parts is the strong accumulation of a DUF642-conserved domain containing protein in the apical region of the stem, which suggests a particular role of this protein during the early development of stem tissues.
31430995	8	7	theme	stem	1222:1225	arg1	segment					1227:1233	the oldest stem segment	1211:1233	the oldest stem segment	1211:1233	Therefore, remobilization of nutrients appears to be an important process in the oldest stem segment.
31430995	6	8	theme	proteolytic	943:953	arg1	activity					955:962	proteolytic activity	943:962	proteolytic activity	943:962	Proteins that bind carbohydrates and have proteolytic activity, as well as enzymes involved in glycan remobilization, accumulate in the basal stem region.
31430995	3	9	theme	wall	465:468	arg1	process					445:451	the development process	429:451	the development process of the cell wall	429:468	This study of cell wall protein-enriched fractions forms the basis for a description of the development process of the cell wall and the linking cell wall localized proteins with the evolution of cell wall composition and structure.
31430995	5	10	theme	apical	805:810	arg1	regions					834:840	the apical and intermediate stem regions	801:840	the apical and intermediate stem regions	801:840	Although the highest similarities were observed between the apical and intermediate stem regions, the proteome patterns are characteristic for each region.
31430995	4	11	theme	cell	603:606	arg1	proteins					613:620	cell wall proteins	603:620	cell wall proteins	603:620	The sequential extraction of cell wall proteins with CaCl2, EGTA, and LiCl-complemented buffers was combined with a gel-based proteome approach and multivariate analysis.
31430995	10	12	theme	early	1770:1774	arg1	development					1776:1786	the early development	1766:1786	the early development of stem tissues	1766:1802	However, the most striking change between the different stem parts is the strong accumulation of a DUF642-conserved domain containing protein in the apical region of the stem, which suggests a particular role of this protein during the early development of stem tissues.
31430995	5	13	theme	intermediate	816:827	arg1	regions					834:840	the apical and intermediate stem regions	801:840	the apical and intermediate stem regions	801:840	Although the highest similarities were observed between the apical and intermediate stem regions, the proteome patterns are characteristic for each region.
31430995	10	14	theme	stem	1590:1593	arg1	parts					1595:1599	the different stem parts	1576:1599	the different stem parts	1576:1599	However, the most striking change between the different stem parts is the strong accumulation of a DUF642-conserved domain containing protein in the apical region of the stem, which suggests a particular role of this protein during the early development of stem tissues.
31430995	3	15	theme	cell	537:540	arg1	wall					542:545	cell wall composition and structure	537:571	cell wall composition and structure	537:571	This study of cell wall protein-enriched fractions forms the basis for a description of the development process of the cell wall and the linking cell wall localized proteins with the evolution of cell wall composition and structure.
31430995	1	16	theme	cell-wall-enriched	87:104	arg1	subproteomes					106:117	the cell-wall-enriched subproteomes	83:117	the cell-wall-enriched subproteomes at three different heights of alfalfa stems	83:161	In this study, the cell-wall-enriched subproteomes at three different heights of alfalfa stems were compared.
31430995	7	17	theme	basal	1114:1118	arg1	segment					1125:1131	the basal stem segment	1110:1131	the basal stem segment	1110:1131	Beta-amylase and ferritin likewise accumulate more in the basal stem segment.
31430995	10	18	theme	tissues	1796:1802	arg1	development					1776:1786	the early development	1766:1786	the early development of stem tissues	1766:1802	However, the most striking change between the different stem parts is the strong accumulation of a DUF642-conserved domain containing protein in the apical region of the stem, which suggests a particular role of this protein during the early development of stem tissues.
31430995	0	19	theme	Developing	42:51	arg1	Stems					61:65	Developing Alfalfa Stems	42:65	Developing Alfalfa Stems	42:65	The Dynamics of the Cell Wall Proteome of Developing Alfalfa Stems.
31430995	9	20	theme	BURP-domain	1470:1480	arg1	subunit					1525:1531	the BURP-domain containing polygalacturonase non-catalytic subunit	1466:1531	the BURP-domain containing polygalacturonase non-catalytic subunit	1466:1531	The intermediate and apical regions are sites of cell wall polymer remodeling, as suggested by the high abundance of proteins involved in the remodeling of the cell wall, such as xyloglucan endoglucosylase, beta-galactosidase, or the BURP-domain containing polygalacturonase non-catalytic subunit.
31430995	0	21	theme	Stems	61:65	arg1	Proteome					30:37	the Cell Wall Proteome	16:37	the Cell Wall Proteome of Developing Alfalfa Stems	16:65	The Dynamics of the Cell Wall Proteome of Developing Alfalfa Stems.
31430995	1	22	from	heights	138:144	arg1	subproteomes					106:117	the cell-wall-enriched subproteomes	83:117	the cell-wall-enriched subproteomes at three different heights of alfalfa stems	83:161	In this study, the cell-wall-enriched subproteomes at three different heights of alfalfa stems were compared.
31430995	9	23	theme	polygalacturonase	1493:1509	arg1	subunit					1525:1531	the BURP-domain containing polygalacturonase non-catalytic subunit	1466:1531	the BURP-domain containing polygalacturonase non-catalytic subunit	1466:1531	The intermediate and apical regions are sites of cell wall polymer remodeling, as suggested by the high abundance of proteins involved in the remodeling of the cell wall, such as xyloglucan endoglucosylase, beta-galactosidase, or the BURP-domain containing polygalacturonase non-catalytic subunit.
31430995	2	24	theme	stem	238:241	arg1	development					243:253	stem development	238:253	stem development	238:253	Since these three heights correspond to different states in stem development, a view on the dynamics of the cell wall proteome during cell maturation is obtained.
31430995	9	25	theme	cell	1285:1288	arg1	remodeling					1303:1312	cell wall polymer remodeling	1285:1312	cell wall polymer remodeling	1285:1312	The intermediate and apical regions are sites of cell wall polymer remodeling, as suggested by the high abundance of proteins involved in the remodeling of the cell wall, such as xyloglucan endoglucosylase, beta-galactosidase, or the BURP-domain containing polygalacturonase non-catalytic subunit.
31430995	0	26	theme	Wall	25:28	arg1	Proteome					30:37	the Cell Wall Proteome	16:37	the Cell Wall Proteome of Developing Alfalfa Stems	16:65	The Dynamics of the Cell Wall Proteome of Developing Alfalfa Stems.
31430995	6	27	theme	stem	1043:1046	arg1	region					1048:1053	the basal stem region	1033:1053	the basal stem region	1033:1053	Proteins that bind carbohydrates and have proteolytic activity, as well as enzymes involved in glycan remobilization, accumulate in the basal stem region.
31430995	9	28	theme	polymer	1295:1301	arg1	remodeling					1303:1312	cell wall polymer remodeling	1285:1312	cell wall polymer remodeling	1285:1312	The intermediate and apical regions are sites of cell wall polymer remodeling, as suggested by the high abundance of proteins involved in the remodeling of the cell wall, such as xyloglucan endoglucosylase, beta-galactosidase, or the BURP-domain containing polygalacturonase non-catalytic subunit.
31430995	4	29	theme	proteome	700:707	arg1	approach					709:716	a gel-based proteome approach	688:716	a gel-based proteome approach	688:716	The sequential extraction of cell wall proteins with CaCl2, EGTA, and LiCl-complemented buffers was combined with a gel-based proteome approach and multivariate analysis.
31430995	3	30	theme	protein-enriched	365:380	arg1	fractions					382:390	cell wall protein-enriched fractions	355:390	cell wall protein-enriched fractions	355:390	This study of cell wall protein-enriched fractions forms the basis for a description of the development process of the cell wall and the linking cell wall localized proteins with the evolution of cell wall composition and structure.
31430995	10	31	theme	domain	1650:1655	arg1	change					1561:1566	the most striking change	1543:1566	the most striking change between the different stem parts	1543:1599	However, the most striking change between the different stem parts is the strong accumulation of a DUF642-conserved domain containing protein in the apical region of the stem, which suggests a particular role of this protein during the early development of stem tissues.
31430995	10	31	theme	domain	1650:1655	arg1	accumulation					1615:1626	the strong accumulation	1604:1626	the strong accumulation of a DUF642-conserved domain containing protein in the apical region of the stem, which suggests a particular role of this protein during the early development of stem tissues	1604:1802	However, the most striking change between the different stem parts is the strong accumulation of a DUF642-conserved domain containing protein in the apical region of the stem, which suggests a particular role of this protein during the early development of stem tissues.
31430995	5	32	theme	stem	829:832	arg1	regions					834:840	the apical and intermediate stem regions	801:840	the apical and intermediate stem regions	801:840	Although the highest similarities were observed between the apical and intermediate stem regions, the proteome patterns are characteristic for each region.
31430995	0	33	theme	Cell	20:23	arg1	Proteome					30:37	the Cell Wall Proteome	16:37	the Cell Wall Proteome of Developing Alfalfa Stems	16:65	The Dynamics of the Cell Wall Proteome of Developing Alfalfa Stems.
31430995	3	34	theme	cell	355:358	arg1	fractions					382:390	cell wall protein-enriched fractions	355:390	cell wall protein-enriched fractions	355:390	This study of cell wall protein-enriched fractions forms the basis for a description of the development process of the cell wall and the linking cell wall localized proteins with the evolution of cell wall composition and structure.
31430995	5	35	theme	highest	758:764	arg1	similarities					766:777	the highest similarities	754:777	the highest similarities	754:777	Although the highest similarities were observed between the apical and intermediate stem regions, the proteome patterns are characteristic for each region.
31430995	2	36	theme	wall	291:294	arg1	proteome					296:303	the cell wall proteome	282:303	the cell wall proteome	282:303	Since these three heights correspond to different states in stem development, a view on the dynamics of the cell wall proteome during cell maturation is obtained.
31430995	1	37	theme	different	128:136	arg1	heights					138:144	three different heights	122:144	three different heights of alfalfa stems	122:161	In this study, the cell-wall-enriched subproteomes at three different heights of alfalfa stems were compared.
31430995	10	38	theme	DUF642-conserved	1633:1648	arg1	domain					1650:1655	a DUF642-conserved domain	1631:1655	a DUF642-conserved domain containing protein in the apical region of the stem, which suggests a particular role of this protein during the early development of stem tissues	1631:1802	However, the most striking change between the different stem parts is the strong accumulation of a DUF642-conserved domain containing protein in the apical region of the stem, which suggests a particular role of this protein during the early development of stem tissues.
31430995	9	39	theme	cell	1396:1399	arg1	wall					1401:1404	the cell wall	1392:1404	the cell wall	1392:1404	The intermediate and apical regions are sites of cell wall polymer remodeling, as suggested by the high abundance of proteins involved in the remodeling of the cell wall, such as xyloglucan endoglucosylase, beta-galactosidase, or the BURP-domain containing polygalacturonase non-catalytic subunit.
31430995	9	40	theme	proteins	1353:1360	arg1	abundance					1340:1348	the high abundance	1331:1348	the high abundance of proteins involved in the remodeling of the cell wall, such as xyloglucan endoglucosylase, beta-galactosidase, or the BURP-domain containing polygalacturonase non-catalytic subunit	1331:1531	The intermediate and apical regions are sites of cell wall polymer remodeling, as suggested by the high abundance of proteins involved in the remodeling of the cell wall, such as xyloglucan endoglucosylase, beta-galactosidase, or the BURP-domain containing polygalacturonase non-catalytic subunit.
31430995	3	41	theme	cell	460:463	arg1	wall					465:468	the cell wall	456:468	the cell wall	456:468	This study of cell wall protein-enriched fractions forms the basis for a description of the development process of the cell wall and the linking cell wall localized proteins with the evolution of cell wall composition and structure.
31430995	10	42	theme	striking	1552:1559	arg1	change					1561:1566	the most striking change	1543:1566	the most striking change between the different stem parts	1543:1599	However, the most striking change between the different stem parts is the strong accumulation of a DUF642-conserved domain containing protein in the apical region of the stem, which suggests a particular role of this protein during the early development of stem tissues.
31430995	10	42	theme	striking	1552:1559	arg1	accumulation					1615:1626	the strong accumulation	1604:1626	the strong accumulation of a DUF642-conserved domain containing protein in the apical region of the stem, which suggests a particular role of this protein during the early development of stem tissues	1604:1802	However, the most striking change between the different stem parts is the strong accumulation of a DUF642-conserved domain containing protein in the apical region of the stem, which suggests a particular role of this protein during the early development of stem tissues.
31430995	3	43	theme	wall	491:494	arg1	proteins					506:513	a description of the development process of the cell wall and the linking cell wall localized proteins	412:513	a description of the development process of the cell wall and the linking cell wall localized proteins with the evolution of cell wall composition and structure	412:571	This study of cell wall protein-enriched fractions forms the basis for a description of the development process of the cell wall and the linking cell wall localized proteins with the evolution of cell wall composition and structure.
31430995	8	44	from	process	1200:1206	arg1	segment					1227:1233	the oldest stem segment	1211:1233	the oldest stem segment	1211:1233	Therefore, remobilization of nutrients appears to be an important process in the oldest stem segment.
31430995	3	45	theme	linking	478:484	arg1	wall					491:494	the linking cell wall	474:494	the linking cell wall	474:494	This study of cell wall protein-enriched fractions forms the basis for a description of the development process of the cell wall and the linking cell wall localized proteins with the evolution of cell wall composition and structure.
31430995	9	46	theme	wall	1401:1404	arg1	remodeling					1378:1387	the remodeling	1374:1387	the remodeling of the cell wall	1374:1404	The intermediate and apical regions are sites of cell wall polymer remodeling, as suggested by the high abundance of proteins involved in the remodeling of the cell wall, such as xyloglucan endoglucosylase, beta-galactosidase, or the BURP-domain containing polygalacturonase non-catalytic subunit.
31430995	1	47	theme	alfalfa	149:155	arg1	stems					157:161	alfalfa stems	149:161	alfalfa stems	149:161	In this study, the cell-wall-enriched subproteomes at three different heights of alfalfa stems were compared.
31430995	0	48	theme	Proteome	30:37	arg1	Dynamics					4:11	The Dynamics	0:11	The Dynamics of the Cell Wall Proteome of Developing Alfalfa Stems.	0:66	The Dynamics of the Cell Wall Proteome of Developing Alfalfa Stems.
31430995	4	49	theme	wall	608:611	arg1	proteins					613:620	cell wall proteins	603:620	cell wall proteins	603:620	The sequential extraction of cell wall proteins with CaCl2, EGTA, and LiCl-complemented buffers was combined with a gel-based proteome approach and multivariate analysis.
31430995	10	50	theme	different	1580:1588	arg1	parts					1595:1599	the different stem parts	1576:1599	the different stem parts	1576:1599	However, the most striking change between the different stem parts is the strong accumulation of a DUF642-conserved domain containing protein in the apical region of the stem, which suggests a particular role of this protein during the early development of stem tissues.
31430995	10	51	theme	protein	1751:1757	arg1	role					1738:1741	a particular role	1725:1741	a particular role of this protein during the early development of stem tissues	1725:1802	However, the most striking change between the different stem parts is the strong accumulation of a DUF642-conserved domain containing protein in the apical region of the stem, which suggests a particular role of this protein during the early development of stem tissues.
31430995	3	52	dep	wall	542:545	arg1	structure					563:571	structure	563:571	structure	563:571	This study of cell wall protein-enriched fractions forms the basis for a description of the development process of the cell wall and the linking cell wall localized proteins with the evolution of cell wall composition and structure.
31430995	3	52	dep	wall	542:545	arg1	composition					547:557	composition	547:557	composition	547:557	This study of cell wall protein-enriched fractions forms the basis for a description of the development process of the cell wall and the linking cell wall localized proteins with the evolution of cell wall composition and structure.
31430995	8	53	theme	oldest	1215:1220	arg1	segment					1227:1233	the oldest stem segment	1211:1233	the oldest stem segment	1211:1233	Therefore, remobilization of nutrients appears to be an important process in the oldest stem segment.
31430995	10	54	theme	particular	1727:1736	arg1	role					1738:1741	a particular role	1725:1741	a particular role of this protein during the early development of stem tissues	1725:1802	However, the most striking change between the different stem parts is the strong accumulation of a DUF642-conserved domain containing protein in the apical region of the stem, which suggests a particular role of this protein during the early development of stem tissues.
31430995	4	55	theme	sequential	578:587	arg1	extraction					589:598	The sequential extraction	574:598	The sequential extraction of cell wall proteins with CaCl2, EGTA, and LiCl-complemented buffers	574:668	The sequential extraction of cell wall proteins with CaCl2, EGTA, and LiCl-complemented buffers was combined with a gel-based proteome approach and multivariate analysis.
31430995	10	56	theme	apical	1683:1688	arg1	region					1690:1695	the apical region	1679:1695	the apical region of the stem, which suggests a particular role of this protein during the early development of stem tissues	1679:1802	However, the most striking change between the different stem parts is the strong accumulation of a DUF642-conserved domain containing protein in the apical region of the stem, which suggests a particular role of this protein during the early development of stem tissues.
31430995	2	57	theme	different	218:226	arg1	states					228:233	different states	218:233	different states in stem development	218:253	Since these three heights correspond to different states in stem development, a view on the dynamics of the cell wall proteome during cell maturation is obtained.
31430995	3	58	theme	wall	542:545	arg1	evolution					524:532	the evolution	520:532	the evolution of cell wall composition and structure	520:571	This study of cell wall protein-enriched fractions forms the basis for a description of the development process of the cell wall and the linking cell wall localized proteins with the evolution of cell wall composition and structure.
31430995	8	59	theme	important	1190:1198	arg1	remobilization					1145:1158	remobilization	1145:1158	remobilization of nutrients	1145:1171	Therefore, remobilization of nutrients appears to be an important process in the oldest stem segment.
31430995	8	59	theme	important	1190:1198	arg1	process					1200:1206	an important process	1187:1206	an important process	1187:1206	Therefore, remobilization of nutrients appears to be an important process in the oldest stem segment.
31430995	4	60	theme	LiCl-complemented	644:660	arg1	buffers					662:668	LiCl-complemented buffers	644:668	LiCl-complemented buffers	644:668	The sequential extraction of cell wall proteins with CaCl2, EGTA, and LiCl-complemented buffers was combined with a gel-based proteome approach and multivariate analysis.
31430995	6	61	theme	glycan	996:1001	arg1	remobilization					1003:1016	glycan remobilization	996:1016	glycan remobilization	996:1016	Proteins that bind carbohydrates and have proteolytic activity, as well as enzymes involved in glycan remobilization, accumulate in the basal stem region.
31430995	3	62	theme	development	433:443	arg1	process					445:451	the development process	429:451	the development process of the cell wall	429:468	This study of cell wall protein-enriched fractions forms the basis for a description of the development process of the cell wall and the linking cell wall localized proteins with the evolution of cell wall composition and structure.
31430995	0	63	theme	Alfalfa	53:59	arg1	Stems					61:65	Developing Alfalfa Stems	42:65	Developing Alfalfa Stems	42:65	The Dynamics of the Cell Wall Proteome of Developing Alfalfa Stems.
31430995	9	64	theme	intermediate	1240:1251	arg1	regions					1264:1270	The intermediate and apical regions	1236:1270	The intermediate and apical regions	1236:1270	The intermediate and apical regions are sites of cell wall polymer remodeling, as suggested by the high abundance of proteins involved in the remodeling of the cell wall, such as xyloglucan endoglucosylase, beta-galactosidase, or the BURP-domain containing polygalacturonase non-catalytic subunit.
31430995	9	64	theme	intermediate	1240:1251	arg1	remodeling					1303:1312	cell wall polymer remodeling	1285:1312	cell wall polymer remodeling	1285:1312	The intermediate and apical regions are sites of cell wall polymer remodeling, as suggested by the high abundance of proteins involved in the remodeling of the cell wall, such as xyloglucan endoglucosylase, beta-galactosidase, or the BURP-domain containing polygalacturonase non-catalytic subunit.
31430995	9	64	theme	intermediate	1240:1251	arg1	sites					1276:1280	sites	1276:1280	sites of cell wall polymer remodeling	1276:1312	The intermediate and apical regions are sites of cell wall polymer remodeling, as suggested by the high abundance of proteins involved in the remodeling of the cell wall, such as xyloglucan endoglucosylase, beta-galactosidase, or the BURP-domain containing polygalacturonase non-catalytic subunit.
31430995	10	65	theme	stem	1791:1794	arg1	tissues					1796:1802	stem tissues	1791:1802	stem tissues	1791:1802	However, the most striking change between the different stem parts is the strong accumulation of a DUF642-conserved domain containing protein in the apical region of the stem, which suggests a particular role of this protein during the early development of stem tissues.
31430995	5	66	theme	proteome	847:854	arg1	characteristic					869:882	characteristic	869:882	characteristic	869:882	Although the highest similarities were observed between the apical and intermediate stem regions, the proteome patterns are characteristic for each region.
31430995	5	66	theme	proteome	847:854	arg1	patterns					856:863	the proteome patterns	843:863	the proteome patterns	843:863	Although the highest similarities were observed between the apical and intermediate stem regions, the proteome patterns are characteristic for each region.
31430995	2	67	from	view	258:261	arg1	dynamics					270:277	the dynamics	266:277	the dynamics of the cell wall proteome during cell maturation	266:326	Since these three heights correspond to different states in stem development, a view on the dynamics of the cell wall proteome during cell maturation is obtained.
31430995	9	68	theme	apical	1257:1262	arg1	regions					1264:1270	The intermediate and apical regions	1236:1270	The intermediate and apical regions	1236:1270	The intermediate and apical regions are sites of cell wall polymer remodeling, as suggested by the high abundance of proteins involved in the remodeling of the cell wall, such as xyloglucan endoglucosylase, beta-galactosidase, or the BURP-domain containing polygalacturonase non-catalytic subunit.
31430995	9	68	theme	apical	1257:1262	arg1	remodeling					1303:1312	cell wall polymer remodeling	1285:1312	cell wall polymer remodeling	1285:1312	The intermediate and apical regions are sites of cell wall polymer remodeling, as suggested by the high abundance of proteins involved in the remodeling of the cell wall, such as xyloglucan endoglucosylase, beta-galactosidase, or the BURP-domain containing polygalacturonase non-catalytic subunit.
31430995	9	68	theme	apical	1257:1262	arg1	sites					1276:1280	sites	1276:1280	sites of cell wall polymer remodeling	1276:1312	The intermediate and apical regions are sites of cell wall polymer remodeling, as suggested by the high abundance of proteins involved in the remodeling of the cell wall, such as xyloglucan endoglucosylase, beta-galactosidase, or the BURP-domain containing polygalacturonase non-catalytic subunit.
31430995	9	69	theme	containing	1482:1491	arg1	subunit					1525:1531	the BURP-domain containing polygalacturonase non-catalytic subunit	1466:1531	the BURP-domain containing polygalacturonase non-catalytic subunit	1466:1531	The intermediate and apical regions are sites of cell wall polymer remodeling, as suggested by the high abundance of proteins involved in the remodeling of the cell wall, such as xyloglucan endoglucosylase, beta-galactosidase, or the BURP-domain containing polygalacturonase non-catalytic subunit.
31430995	9	70	theme	non-catalytic	1511:1523	arg1	subunit					1525:1531	the BURP-domain containing polygalacturonase non-catalytic subunit	1466:1531	the BURP-domain containing polygalacturonase non-catalytic subunit	1466:1531	The intermediate and apical regions are sites of cell wall polymer remodeling, as suggested by the high abundance of proteins involved in the remodeling of the cell wall, such as xyloglucan endoglucosylase, beta-galactosidase, or the BURP-domain containing polygalacturonase non-catalytic subunit.
31430995	2	71	theme	cell	286:289	arg1	proteome					296:303	the cell wall proteome	282:303	the cell wall proteome	282:303	Since these three heights correspond to different states in stem development, a view on the dynamics of the cell wall proteome during cell maturation is obtained.
31430995	4	72	theme	multivariate	722:733	arg1	analysis					735:742	multivariate analysis	722:742	multivariate analysis	722:742	The sequential extraction of cell wall proteins with CaCl2, EGTA, and LiCl-complemented buffers was combined with a gel-based proteome approach and multivariate analysis.
31430995	3	73	with	proteins	506:513	arg1	evolution					524:532	the evolution	520:532	the evolution of cell wall composition and structure	520:571	This study of cell wall protein-enriched fractions forms the basis for a description of the development process of the cell wall and the linking cell wall localized proteins with the evolution of cell wall composition and structure.
31430995	9	74	theme	wall	1290:1293	arg1	remodeling					1303:1312	cell wall polymer remodeling	1285:1312	cell wall polymer remodeling	1285:1312	The intermediate and apical regions are sites of cell wall polymer remodeling, as suggested by the high abundance of proteins involved in the remodeling of the cell wall, such as xyloglucan endoglucosylase, beta-galactosidase, or the BURP-domain containing polygalacturonase non-catalytic subunit.
31430995	6	75	theme	basal	1037:1041	arg1	region					1048:1053	the basal stem region	1033:1053	the basal stem region	1033:1053	Proteins that bind carbohydrates and have proteolytic activity, as well as enzymes involved in glycan remobilization, accumulate in the basal stem region.
31430995	10	76	theme	strong	1608:1613	arg1	change					1561:1566	the most striking change	1543:1566	the most striking change between the different stem parts	1543:1599	However, the most striking change between the different stem parts is the strong accumulation of a DUF642-conserved domain containing protein in the apical region of the stem, which suggests a particular role of this protein during the early development of stem tissues.
31430995	10	76	theme	strong	1608:1613	arg1	accumulation					1615:1626	the strong accumulation	1604:1626	the strong accumulation of a DUF642-conserved domain containing protein in the apical region of the stem, which suggests a particular role of this protein during the early development of stem tissues	1604:1802	However, the most striking change between the different stem parts is the strong accumulation of a DUF642-conserved domain containing protein in the apical region of the stem, which suggests a particular role of this protein during the early development of stem tissues.
31430995	9	77	theme	remodeling	1303:1312	arg1	regions					1264:1270	The intermediate and apical regions	1236:1270	The intermediate and apical regions	1236:1270	The intermediate and apical regions are sites of cell wall polymer remodeling, as suggested by the high abundance of proteins involved in the remodeling of the cell wall, such as xyloglucan endoglucosylase, beta-galactosidase, or the BURP-domain containing polygalacturonase non-catalytic subunit.
31430995	9	77	theme	remodeling	1303:1312	arg1	remodeling					1303:1312	cell wall polymer remodeling	1285:1312	cell wall polymer remodeling	1285:1312	The intermediate and apical regions are sites of cell wall polymer remodeling, as suggested by the high abundance of proteins involved in the remodeling of the cell wall, such as xyloglucan endoglucosylase, beta-galactosidase, or the BURP-domain containing polygalacturonase non-catalytic subunit.
31430995	9	77	theme	remodeling	1303:1312	arg1	sites					1276:1280	sites	1276:1280	sites of cell wall polymer remodeling	1276:1312	The intermediate and apical regions are sites of cell wall polymer remodeling, as suggested by the high abundance of proteins involved in the remodeling of the cell wall, such as xyloglucan endoglucosylase, beta-galactosidase, or the BURP-domain containing polygalacturonase non-catalytic subunit.
31430995	2	78	from	states	228:233	arg1	development					243:253	stem development	238:253	stem development	238:253	Since these three heights correspond to different states in stem development, a view on the dynamics of the cell wall proteome during cell maturation is obtained.
31430995	4	79	theme	gel-based	690:698	arg1	approach					709:716	a gel-based proteome approach	688:716	a gel-based proteome approach	688:716	The sequential extraction of cell wall proteins with CaCl2, EGTA, and LiCl-complemented buffers was combined with a gel-based proteome approach and multivariate analysis.
31430995	7	80	theme	stem	1120:1123	arg1	segment					1125:1131	the basal stem segment	1110:1131	the basal stem segment	1110:1131	Beta-amylase and ferritin likewise accumulate more in the basal stem segment.
31430995	9	81	theme	xyloglucan	1415:1424	arg1	endoglucosylase					1426:1440	xyloglucan endoglucosylase	1415:1440	xyloglucan endoglucosylase	1415:1440	The intermediate and apical regions are sites of cell wall polymer remodeling, as suggested by the high abundance of proteins involved in the remodeling of the cell wall, such as xyloglucan endoglucosylase, beta-galactosidase, or the BURP-domain containing polygalacturonase non-catalytic subunit.
31430995	3	82	theme	fractions	382:390	arg1	study					346:350	This study	341:350	This study of cell wall protein-enriched fractions	341:390	This study of cell wall protein-enriched fractions forms the basis for a description of the development process of the cell wall and the linking cell wall localized proteins with the evolution of cell wall composition and structure.
31430995	6	83	contain	have	938:941	arg2	activity					955:962	proteolytic activity	943:962	proteolytic activity	943:962	Proteins that bind carbohydrates and have proteolytic activity, as well as enzymes involved in glycan remobilization, accumulate in the basal stem region.
31430995	6	83	contain	have	938:941	arg1	Proteins					901:908	Proteins	901:908	Proteins that bind carbohydrates and have proteolytic activity	901:962	Proteins that bind carbohydrates and have proteolytic activity, as well as enzymes involved in glycan remobilization, accumulate in the basal stem region.
31430995	3	84	theme	description	414:424	arg1	proteins					506:513	a description of the development process of the cell wall and the linking cell wall localized proteins	412:513	a description of the development process of the cell wall and the linking cell wall localized proteins with the evolution of cell wall composition and structure	412:571	This study of cell wall protein-enriched fractions forms the basis for a description of the development process of the cell wall and the linking cell wall localized proteins with the evolution of cell wall composition and structure.
31430995	3	85	theme	wall	360:363	arg1	fractions					382:390	cell wall protein-enriched fractions	355:390	cell wall protein-enriched fractions	355:390	This study of cell wall protein-enriched fractions forms the basis for a description of the development process of the cell wall and the linking cell wall localized proteins with the evolution of cell wall composition and structure.
31430995	2	86	theme	proteome	296:303	arg1	dynamics					270:277	the dynamics	266:277	the dynamics of the cell wall proteome during cell maturation	266:326	Since these three heights correspond to different states in stem development, a view on the dynamics of the cell wall proteome during cell maturation is obtained.
31430995	9	87	theme	high	1335:1338	arg1	abundance					1340:1348	the high abundance	1331:1348	the high abundance of proteins involved in the remodeling of the cell wall, such as xyloglucan endoglucosylase, beta-galactosidase, or the BURP-domain containing polygalacturonase non-catalytic subunit	1331:1531	The intermediate and apical regions are sites of cell wall polymer remodeling, as suggested by the high abundance of proteins involved in the remodeling of the cell wall, such as xyloglucan endoglucosylase, beta-galactosidase, or the BURP-domain containing polygalacturonase non-catalytic subunit.
31430995	4	88	with	extraction	589:598	arg1	buffers					662:668	LiCl-complemented buffers	644:668	LiCl-complemented buffers	644:668	The sequential extraction of cell wall proteins with CaCl2, EGTA, and LiCl-complemented buffers was combined with a gel-based proteome approach and multivariate analysis.
31430995	4	88	with	extraction	589:598	arg1	EGTA					634:637	EGTA	634:637	EGTA	634:637	The sequential extraction of cell wall proteins with CaCl2, EGTA, and LiCl-complemented buffers was combined with a gel-based proteome approach and multivariate analysis.
31430995	4	88	with	extraction	589:598	arg1	CaCl2					627:631	CaCl2	627:631	CaCl2	627:631	The sequential extraction of cell wall proteins with CaCl2, EGTA, and LiCl-complemented buffers was combined with a gel-based proteome approach and multivariate analysis.
31430995	3	89	theme	process	445:451	arg1	wall					491:494	the linking cell wall	474:494	the linking cell wall	474:494	This study of cell wall protein-enriched fractions forms the basis for a description of the development process of the cell wall and the linking cell wall localized proteins with the evolution of cell wall composition and structure.
31430995	3	89	theme	process	445:451	arg1	description					414:424	a description	412:424	a description of the development process of the cell wall	412:468	This study of cell wall protein-enriched fractions forms the basis for a description of the development process of the cell wall and the linking cell wall localized proteins with the evolution of cell wall composition and structure.
31430995	10	90	theme	stem	1704:1707	arg1	region					1690:1695	the apical region	1679:1695	the apical region of the stem, which suggests a particular role of this protein during the early development of stem tissues	1679:1802	However, the most striking change between the different stem parts is the strong accumulation of a DUF642-conserved domain containing protein in the apical region of the stem, which suggests a particular role of this protein during the early development of stem tissues.
30879649	6	0	theme	multiple	1198:1205	arg1	arms					1207:1210	multiple arms	1198:1210	multiple arms of immune responses	1198:1230	The results revealed that CS/O-HTCC nanoparticles as a type of multifunctional adjuvant can improve multiple arms of immune responses.
30879649	6	0	theme	multiple	1198:1205	arg1	responses					1222:1230	immune responses	1215:1230	immune responses	1215:1230	The results revealed that CS/O-HTCC nanoparticles as a type of multifunctional adjuvant can improve multiple arms of immune responses.
30879649	2	1	theme	chloride	499:506	arg1	nanoparticles					414:426	the nanoparticles	410:426	the nanoparticles of curdlan sulfate/O-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride (CS/O-HTCC)	410:518	We have proven that compared with antigens loaded on CS or O-HTCC alone in nasal vaccination, antigens loaded on the nanoparticles of curdlan sulfate/O-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride (CS/O-HTCC) can induce stronger systemic and mucosal immune responses.
30879649	5	2	theme	PI3K/AKT	981:988	arg1	pathways					999:1006	the PI3K/AKT and MAPK pathways	977:1006	pathways	999:1006	CS/O-HTCC nanoparticles also activated the PI3K/AKT and MAPK pathways and significantly promoted IL-2 transcription to induce the proliferation of lymphocytes.
30879649	0	3	theme	immune	115:120	arg1	responses					122:130	immune responses	115:130	immune responses	115:130	Curdlan sulfate/O-linked quaternized chitosan nanoparticles acting as potential adjuvants promote multiple arms of immune responses.
30879649	1	4	theme	effective	276:284	arg1	adjuvants					286:294	effective adjuvants	276:294	effective adjuvants	276:294	Nasal immunization to prevent and treat diseases caused by infection through the respiratory tract cannot be actualized because of the lack of effective adjuvants.
30879649	0	5	link	sulfate/O-linked	8:23	arg1	nanoparticles					46:58	Curdlan sulfate/O-linked quaternized chitosan nanoparticles	0:58	Curdlan sulfate/O-linked quaternized chitosan nanoparticles acting as potential adjuvants	0:88	Curdlan sulfate/O-linked quaternized chitosan nanoparticles acting as potential adjuvants promote multiple arms of immune responses.
30879649	1	6	theme	adjuvants	286:294	arg1	lack					268:271	the lack	264:271	the lack of effective adjuvants	264:294	Nasal immunization to prevent and treat diseases caused by infection through the respiratory tract cannot be actualized because of the lack of effective adjuvants.
30879649	4	7	theme	type	905:908	arg1	genes					931:935	type I interferon-related genes	905:935	type I interferon-related genes	905:935	The results showed that CS/O-HTCC nanoparticles can improve the activation of antigen-presenting cells, upregulate the production of inflammatory factors and cytokines, induce cross-presentation, and simultaneously activate type I interferon-related genes.
30879649	4	8	theme	factors	827:833	arg1	production					800:809	the production	796:809	the production of inflammatory factors and cytokines	796:847	The results showed that CS/O-HTCC nanoparticles can improve the activation of antigen-presenting cells, upregulate the production of inflammatory factors and cytokines, induce cross-presentation, and simultaneously activate type I interferon-related genes.
30879649	0	9	theme	responses	122:130	arg1	arms					107:110	multiple arms	98:110	multiple arms of immune responses	98:130	Curdlan sulfate/O-linked quaternized chitosan nanoparticles acting as potential adjuvants promote multiple arms of immune responses.
30879649	0	9	theme	responses	122:130	arg1	responses					122:130	immune responses	115:130	immune responses	115:130	Curdlan sulfate/O-linked quaternized chitosan nanoparticles acting as potential adjuvants promote multiple arms of immune responses.
30879649	1	10	dep	immunization	139:150	arg1	treat					167:171	treat	167:171	treat diseases caused by infection through the respiratory tract	167:230	Nasal immunization to prevent and treat diseases caused by infection through the respiratory tract cannot be actualized because of the lack of effective adjuvants.
30879649	1	10	dep	immunization	139:150	arg1	prevent					155:161	prevent	155:161	prevent	155:161	Nasal immunization to prevent and treat diseases caused by infection through the respiratory tract cannot be actualized because of the lack of effective adjuvants.
30879649	6	11	theme	adjuvant	1177:1184	arg1	type					1153:1156	a type	1151:1156	a type of multifunctional adjuvant	1151:1184	The results revealed that CS/O-HTCC nanoparticles as a type of multifunctional adjuvant can improve multiple arms of immune responses.
30879649	0	12	theme	sulfate/O-linked	8:23	arg1	nanoparticles					46:58	Curdlan sulfate/O-linked quaternized chitosan nanoparticles	0:58	Curdlan sulfate/O-linked quaternized chitosan nanoparticles acting as potential adjuvants	0:88	Curdlan sulfate/O-linked quaternized chitosan nanoparticles acting as potential adjuvants promote multiple arms of immune responses.
30879649	0	13	theme	multiple	98:105	arg1	arms					107:110	multiple arms	98:110	multiple arms of immune responses	98:130	Curdlan sulfate/O-linked quaternized chitosan nanoparticles acting as potential adjuvants promote multiple arms of immune responses.
30879649	0	13	theme	multiple	98:105	arg1	responses					122:130	immune responses	115:130	immune responses	115:130	Curdlan sulfate/O-linked quaternized chitosan nanoparticles acting as potential adjuvants promote multiple arms of immune responses.
30879649	4	14	theme	interferon-related	912:929	arg1	genes					931:935	type I interferon-related genes	905:935	type I interferon-related genes	905:935	The results showed that CS/O-HTCC nanoparticles can improve the activation of antigen-presenting cells, upregulate the production of inflammatory factors and cytokines, induce cross-presentation, and simultaneously activate type I interferon-related genes.
30879649	5	15	theme	CS/O-HTCC	938:946	arg1	nanoparticles					948:960	CS/O-HTCC nanoparticles	938:960	CS/O-HTCC nanoparticles	938:960	CS/O-HTCC nanoparticles also activated the PI3K/AKT and MAPK pathways and significantly promoted IL-2 transcription to induce the proliferation of lymphocytes.
30879649	0	16	theme	Curdlan	0:6	arg1	nanoparticles					46:58	Curdlan sulfate/O-linked quaternized chitosan nanoparticles	0:58	Curdlan sulfate/O-linked quaternized chitosan nanoparticles acting as potential adjuvants	0:88	Curdlan sulfate/O-linked quaternized chitosan nanoparticles acting as potential adjuvants promote multiple arms of immune responses.
30879649	4	17	theme	I	910:910	arg1	genes					931:935	type I interferon-related genes	905:935	type I interferon-related genes	905:935	The results showed that CS/O-HTCC nanoparticles can improve the activation of antigen-presenting cells, upregulate the production of inflammatory factors and cytokines, induce cross-presentation, and simultaneously activate type I interferon-related genes.
30879649	4	18	theme	cells	778:782	arg1	activation					745:754	the activation	741:754	the activation of antigen-presenting cells	741:782	The results showed that CS/O-HTCC nanoparticles can improve the activation of antigen-presenting cells, upregulate the production of inflammatory factors and cytokines, induce cross-presentation, and simultaneously activate type I interferon-related genes.
30879649	0	19	theme	chitosan	37:44	arg1	nanoparticles					46:58	Curdlan sulfate/O-linked quaternized chitosan nanoparticles	0:58	Curdlan sulfate/O-linked quaternized chitosan nanoparticles acting as potential adjuvants	0:88	Curdlan sulfate/O-linked quaternized chitosan nanoparticles acting as potential adjuvants promote multiple arms of immune responses.
30879649	2	20	dep	systemic	540:547	arg1	stronger					531:538	stronger	531:538	stronger	531:538	We have proven that compared with antigens loaded on CS or O-HTCC alone in nasal vaccination, antigens loaded on the nanoparticles of curdlan sulfate/O-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride (CS/O-HTCC) can induce stronger systemic and mucosal immune responses.
30879649	5	21	theme	IL-2	1035:1038	arg1	transcription					1040:1052	IL-2 transcription	1035:1052	IL-2 transcription	1035:1052	CS/O-HTCC nanoparticles also activated the PI3K/AKT and MAPK pathways and significantly promoted IL-2 transcription to induce the proliferation of lymphocytes.
30879649	1	22	theme	respiratory	214:224	arg1	tract					226:230	the respiratory tract	210:230	the respiratory tract	210:230	Nasal immunization to prevent and treat diseases caused by infection through the respiratory tract cannot be actualized because of the lack of effective adjuvants.
30879649	0	23	theme	quaternized	25:35	arg1	nanoparticles					46:58	Curdlan sulfate/O-linked quaternized chitosan nanoparticles	0:58	Curdlan sulfate/O-linked quaternized chitosan nanoparticles acting as potential adjuvants	0:88	Curdlan sulfate/O-linked quaternized chitosan nanoparticles acting as potential adjuvants promote multiple arms of immune responses.
30879649	2	24	theme	immune	561:566	arg1	responses					568:576	stronger systemic and mucosal immune responses	531:576	stronger systemic and mucosal immune responses	531:576	We have proven that compared with antigens loaded on CS or O-HTCC alone in nasal vaccination, antigens loaded on the nanoparticles of curdlan sulfate/O-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride (CS/O-HTCC) can induce stronger systemic and mucosal immune responses.
30879649	3	25	theme	nanoparticles	666:678	arg1	activity					629:636	the immunostimulatory activity	607:636	the immunostimulatory activity	607:636	In this study, we evaluated the immunostimulatory activity and mechanisms of CS/O-HTCC nanoparticles.
30879649	3	25	theme	nanoparticles	666:678	arg1	mechanisms					642:651	mechanisms	642:651	mechanisms of CS/O-HTCC nanoparticles	642:678	In this study, we evaluated the immunostimulatory activity and mechanisms of CS/O-HTCC nanoparticles.
30879649	2	26	theme	chitosan	490:497	arg1	CS/O-HTCC					509:517	CS/O-HTCC	509:517	CS/O-HTCC	509:517	We have proven that compared with antigens loaded on CS or O-HTCC alone in nasal vaccination, antigens loaded on the nanoparticles of curdlan sulfate/O-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride (CS/O-HTCC) can induce stronger systemic and mucosal immune responses.
30879649	2	26	theme	chitosan	490:497	arg1	chloride					499:506	curdlan sulfate/O-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride	431:506	curdlan sulfate/O-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride (CS/O-HTCC)	431:518	We have proven that compared with antigens loaded on CS or O-HTCC alone in nasal vaccination, antigens loaded on the nanoparticles of curdlan sulfate/O-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride (CS/O-HTCC) can induce stronger systemic and mucosal immune responses.
30879649	2	27	theme	mucosal	553:559	arg1	responses					568:576	stronger systemic and mucosal immune responses	531:576	stronger systemic and mucosal immune responses	531:576	We have proven that compared with antigens loaded on CS or O-HTCC alone in nasal vaccination, antigens loaded on the nanoparticles of curdlan sulfate/O-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride (CS/O-HTCC) can induce stronger systemic and mucosal immune responses.
30879649	2	28	theme	ammonium	481:488	arg1	CS/O-HTCC					509:517	CS/O-HTCC	509:517	CS/O-HTCC	509:517	We have proven that compared with antigens loaded on CS or O-HTCC alone in nasal vaccination, antigens loaded on the nanoparticles of curdlan sulfate/O-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride (CS/O-HTCC) can induce stronger systemic and mucosal immune responses.
30879649	2	28	theme	ammonium	481:488	arg1	chloride					499:506	curdlan sulfate/O-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride	431:506	curdlan sulfate/O-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride (CS/O-HTCC)	431:518	We have proven that compared with antigens loaded on CS or O-HTCC alone in nasal vaccination, antigens loaded on the nanoparticles of curdlan sulfate/O-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride (CS/O-HTCC) can induce stronger systemic and mucosal immune responses.
30879649	2	29	theme	propyl-3-trimethyl	462:479	arg1	CS/O-HTCC					509:517	CS/O-HTCC	509:517	CS/O-HTCC	509:517	We have proven that compared with antigens loaded on CS or O-HTCC alone in nasal vaccination, antigens loaded on the nanoparticles of curdlan sulfate/O-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride (CS/O-HTCC) can induce stronger systemic and mucosal immune responses.
30879649	2	29	theme	propyl-3-trimethyl	462:479	arg1	chloride					499:506	curdlan sulfate/O-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride	431:506	curdlan sulfate/O-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride (CS/O-HTCC)	431:518	We have proven that compared with antigens loaded on CS or O-HTCC alone in nasal vaccination, antigens loaded on the nanoparticles of curdlan sulfate/O-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride (CS/O-HTCC) can induce stronger systemic and mucosal immune responses.
30879649	2	30	theme	systemic	540:547	arg1	responses					568:576	stronger systemic and mucosal immune responses	531:576	stronger systemic and mucosal immune responses	531:576	We have proven that compared with antigens loaded on CS or O-HTCC alone in nasal vaccination, antigens loaded on the nanoparticles of curdlan sulfate/O-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride (CS/O-HTCC) can induce stronger systemic and mucosal immune responses.
30879649	3	31	theme	CS/O-HTCC	656:664	arg1	nanoparticles					666:678	CS/O-HTCC nanoparticles	656:678	CS/O-HTCC nanoparticles	656:678	In this study, we evaluated the immunostimulatory activity and mechanisms of CS/O-HTCC nanoparticles.
30879649	1	32	theme	Nasal	133:137	arg1	immunization					139:150	Nasal immunization	133:150	Nasal immunization to prevent and treat diseases caused by infection through the respiratory tract	133:230	Nasal immunization to prevent and treat diseases caused by infection through the respiratory tract cannot be actualized because of the lack of effective adjuvants.
30879649	5	33	theme	MAPK	994:997	arg1	pathways					999:1006	the PI3K/AKT and MAPK pathways	977:1006	pathways	999:1006	CS/O-HTCC nanoparticles also activated the PI3K/AKT and MAPK pathways and significantly promoted IL-2 transcription to induce the proliferation of lymphocytes.
30879649	4	34	theme	cytokines	839:847	arg1	production					800:809	the production	796:809	the production of inflammatory factors and cytokines	796:847	The results showed that CS/O-HTCC nanoparticles can improve the activation of antigen-presenting cells, upregulate the production of inflammatory factors and cytokines, induce cross-presentation, and simultaneously activate type I interferon-related genes.
30879649	4	35	theme	antigen-presenting	759:776	arg1	cells					778:782	antigen-presenting cells	759:782	antigen-presenting cells	759:782	The results showed that CS/O-HTCC nanoparticles can improve the activation of antigen-presenting cells, upregulate the production of inflammatory factors and cytokines, induce cross-presentation, and simultaneously activate type I interferon-related genes.
30879649	6	36	theme	CS/O-HTCC	1124:1132	arg1	nanoparticles					1134:1146	CS/O-HTCC nanoparticles	1124:1146	CS/O-HTCC nanoparticles as a type of multifunctional adjuvant	1124:1184	The results revealed that CS/O-HTCC nanoparticles as a type of multifunctional adjuvant can improve multiple arms of immune responses.
30879649	4	37	theme	inflammatory	814:825	arg1	factors					827:833	inflammatory factors	814:833	inflammatory factors	814:833	The results showed that CS/O-HTCC nanoparticles can improve the activation of antigen-presenting cells, upregulate the production of inflammatory factors and cytokines, induce cross-presentation, and simultaneously activate type I interferon-related genes.
30879649	5	38	theme	lymphocytes	1085:1095	arg1	proliferation					1068:1080	the proliferation	1064:1080	the proliferation of lymphocytes	1064:1095	CS/O-HTCC nanoparticles also activated the PI3K/AKT and MAPK pathways and significantly promoted IL-2 transcription to induce the proliferation of lymphocytes.
30879649	6	39	theme	immune	1215:1220	arg1	responses					1222:1230	immune responses	1215:1230	immune responses	1215:1230	The results revealed that CS/O-HTCC nanoparticles as a type of multifunctional adjuvant can improve multiple arms of immune responses.
30879649	6	40	theme	responses	1222:1230	arg1	arms					1207:1210	multiple arms	1198:1210	multiple arms of immune responses	1198:1230	The results revealed that CS/O-HTCC nanoparticles as a type of multifunctional adjuvant can improve multiple arms of immune responses.
30879649	6	40	theme	responses	1222:1230	arg1	responses					1222:1230	immune responses	1215:1230	immune responses	1215:1230	The results revealed that CS/O-HTCC nanoparticles as a type of multifunctional adjuvant can improve multiple arms of immune responses.
30879649	3	41	theme	immunostimulatory	611:627	arg1	activity					629:636	the immunostimulatory activity	607:636	the immunostimulatory activity	607:636	In this study, we evaluated the immunostimulatory activity and mechanisms of CS/O-HTCC nanoparticles.
30879649	2	42	theme	nasal	372:376	arg1	vaccination					378:388	nasal vaccination	372:388	nasal vaccination	372:388	We have proven that compared with antigens loaded on CS or O-HTCC alone in nasal vaccination, antigens loaded on the nanoparticles of curdlan sulfate/O-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride (CS/O-HTCC) can induce stronger systemic and mucosal immune responses.
30879649	0	43	theme	potential	70:78	arg1	adjuvants					80:88	potential adjuvants	70:88	potential adjuvants	70:88	Curdlan sulfate/O-linked quaternized chitosan nanoparticles acting as potential adjuvants promote multiple arms of immune responses.
30879649	4	44	theme	CS/O-HTCC	705:713	arg1	nanoparticles					715:727	CS/O-HTCC nanoparticles	705:727	CS/O-HTCC nanoparticles	705:727	The results showed that CS/O-HTCC nanoparticles can improve the activation of antigen-presenting cells, upregulate the production of inflammatory factors and cytokines, induce cross-presentation, and simultaneously activate type I interferon-related genes.
29588363	3	0	theme	designed	520:527	arg1	sxc					529:531	rationally designed sxc	509:531	rationally designed sxc catalytically hypomorphic	509:557	To dissect the requirement for O-GlcNAc signaling in Drosophila development, we used CRISPR/Cas9 gene editing to generate rationally designed sxc catalytically hypomorphic or null point mutants.
29588363	1	1	from	modification	78:89	arg1	proteins					141:148	nucleocytoplasmic proteins	123:148	nucleocytoplasmic proteins with GlcNAc (O-GlcNAcylation)	123:178	Post-translational modification of serine/threonine residues in nucleocytoplasmic proteins with GlcNAc (O-GlcNAcylation) is an essential regulatory mechanism in many cellular processes.
29588363	10	2	theme	hypomorphic	1562:1572	arg1	sxc					1581:1583	the endogenous OGT hypomorphic mutant sxc	1543:1583	the endogenous OGT hypomorphic mutant sxc	1543:1583	In conclusion, the generation of the endogenous OGT hypomorphic mutant sxc enabled us to identify pleiotropic effects of globally reduced protein O-GlcNAc during Drosophila development.
29588363	7	3	theme	cell	1124:1127	arg1	dHcf					1137:1140	dHcf	1137:1140	dHcf	1137:1140	Genetic interaction between sxc and a null allele of Drosophila host cell factor (dHcf), encoding an extensively O-GlcNAcylated transcriptional coactivator, resulted in abnormal scutellar bristle numbers.
29588363	7	3	theme	cell	1124:1127	arg1	factor					1129:1134	Drosophila host cell factor	1108:1134	Drosophila host cell factor (dHcf)	1108:1141	Genetic interaction between sxc and a null allele of Drosophila host cell factor (dHcf), encoding an extensively O-GlcNAcylated transcriptional coactivator, resulted in abnormal scutellar bristle numbers.
29588363	3	4	theme	point	567:571	arg1	mutants					573:579	null point mutants	562:579	null point mutants	562:579	To dissect the requirement for O-GlcNAc signaling in Drosophila development, we used CRISPR/Cas9 gene editing to generate rationally designed sxc catalytically hypomorphic or null point mutants.
29588363	2	5	dep	known	326:330	arg1	also					321:324	also	321:324	also	321:324	In Drosophila, null mutants of the Polycomb gene O-GlcNAc transferase (OGT; also known as super sex combs (sxc)) display homeotic phenotypes.
29588363	10	6	theme	endogenous	1547:1556	arg1	sxc					1581:1583	the endogenous OGT hypomorphic mutant sxc	1543:1583	the endogenous OGT hypomorphic mutant sxc	1543:1583	In conclusion, the generation of the endogenous OGT hypomorphic mutant sxc enabled us to identify pleiotropic effects of globally reduced protein O-GlcNAc during Drosophila development.
29588363	7	7	theme	Drosophila	1108:1117	arg1	dHcf					1137:1140	dHcf	1137:1140	dHcf	1137:1140	Genetic interaction between sxc and a null allele of Drosophila host cell factor (dHcf), encoding an extensively O-GlcNAcylated transcriptional coactivator, resulted in abnormal scutellar bristle numbers.
29588363	7	7	theme	Drosophila	1108:1117	arg1	factor					1129:1134	Drosophila host cell factor	1108:1134	Drosophila host cell factor (dHcf)	1108:1141	Genetic interaction between sxc and a null allele of Drosophila host cell factor (dHcf), encoding an extensively O-GlcNAcylated transcriptional coactivator, resulted in abnormal scutellar bristle numbers.
29588363	5	8	theme	hypomorphic	863:873	arg1	mutant					851:856	a second mutant	842:856	a second mutant	842:856	One of these mutants, the catalytically inactive sxc , was recessive lethal, whereas a second mutant, the hypomorphic sxc , was homozygous viable.
29588363	5	8	theme	hypomorphic	863:873	arg1	sxc					875:877	the hypomorphic sxc	859:877	the hypomorphic sxc	859:877	One of these mutants, the catalytically inactive sxc , was recessive lethal, whereas a second mutant, the hypomorphic sxc , was homozygous viable.
29588363	1	9	with	proteins	141:148	arg1	O-GlcNAcylation					163:177	O-GlcNAcylation	163:177	O-GlcNAcylation	163:177	Post-translational modification of serine/threonine residues in nucleocytoplasmic proteins with GlcNAc (O-GlcNAcylation) is an essential regulatory mechanism in many cellular processes.
29588363	1	9	with	proteins	141:148	arg1	GlcNAc					155:160	GlcNAc	155:160	GlcNAc (O-GlcNAcylation)	155:178	Post-translational modification of serine/threonine residues in nucleocytoplasmic proteins with GlcNAc (O-GlcNAcylation) is an essential regulatory mechanism in many cellular processes.
29588363	1	10	theme	cellular	225:232	arg1	processes					234:242	many cellular processes	220:242	many cellular processes	220:242	Post-translational modification of serine/threonine residues in nucleocytoplasmic proteins with GlcNAc (O-GlcNAcylation) is an essential regulatory mechanism in many cellular processes.
29588363	10	11	theme	sxc	1581:1583	arg1	generation					1529:1538	the generation	1525:1538	the generation of the endogenous OGT hypomorphic mutant sxc	1525:1583	In conclusion, the generation of the endogenous OGT hypomorphic mutant sxc enabled us to identify pleiotropic effects of globally reduced protein O-GlcNAc during Drosophila development.
29588363	11	12	theme	substrates	1796:1805	arg1	discovery					1779:1787	discovery	1779:1787	discovery of OGT substrates that are critical for Drosophila development	1779:1850	The mutants generated and phenotypes observed in this study provide a platform for discovery of OGT substrates that are critical for Drosophila development.
29588363	5	13	theme	second	844:849	arg1	mutant					851:856	a second mutant	842:856	a second mutant	842:856	One of these mutants, the catalytically inactive sxc , was recessive lethal, whereas a second mutant, the hypomorphic sxc , was homozygous viable.
29588363	5	13	theme	second	844:849	arg1	viable					896:901	viable	896:901	viable	896:901	One of these mutants, the catalytically inactive sxc , was recessive lethal, whereas a second mutant, the hypomorphic sxc , was homozygous viable.
29588363	5	13	theme	second	844:849	arg1	sxc					875:877	the hypomorphic sxc	859:877	the hypomorphic sxc	859:877	One of these mutants, the catalytically inactive sxc , was recessive lethal, whereas a second mutant, the hypomorphic sxc , was homozygous viable.
29588363	9	14	theme	function	1473:1480	arg1	independent					1445:1455	independent	1445:1455	independent	1445:1455	Interestingly, this phenotype was independent of OGT Polycomb function or dHcf downstream targets.
29588363	4	15	theme	detectable	726:735	arg1	effects					748:754	no detectable off-target effects	723:754	no detectable off-target effects	723:754	Of the fertile males derived from embryos injected with the CRISPR/Cas9 reagents, 25% produced progeny carrying precise point mutations with no detectable off-target effects.
29588363	9	16	theme	dHcf	1485:1488	arg1	targets					1501:1507	dHcf downstream targets	1485:1507	dHcf downstream targets	1485:1507	Interestingly, this phenotype was independent of OGT Polycomb function or dHcf downstream targets.
29588363	6	17	theme	total	929:933	arg1	O-GlcNAcylation					943:957	reduced total protein O-GlcNAcylation	921:957	reduced total protein O-GlcNAcylation in the sxc mutant	921:975	We observed that reduced total protein O-GlcNAcylation in the sxc mutant is associated with a wing vein phenotype and temperature-dependent lethality.
29588363	2	18	theme	gene	289:292	arg1	mutants					265:271	null mutants	260:271	null mutants of the Polycomb gene O-GlcNAc transferase (OGT; also known as super sex combs (sxc))	260:356	In Drosophila, null mutants of the Polycomb gene O-GlcNAc transferase (OGT; also known as super sex combs (sxc)) display homeotic phenotypes.
29588363	9	19	theme	targets	1501:1507	arg1	independent					1445:1455	independent	1445:1455	independent	1445:1455	Interestingly, this phenotype was independent of OGT Polycomb function or dHcf downstream targets.
29588363	4	20	theme	point	702:706	arg1	mutations					708:716	precise point mutations	694:716	precise point mutations	694:716	Of the fertile males derived from embryos injected with the CRISPR/Cas9 reagents, 25% produced progeny carrying precise point mutations with no detectable off-target effects.
29588363	1	21	from	mechanism	207:215	arg1	processes					234:242	many cellular processes	220:242	many cellular processes	220:242	Post-translational modification of serine/threonine residues in nucleocytoplasmic proteins with GlcNAc (O-GlcNAcylation) is an essential regulatory mechanism in many cellular processes.
29588363	6	22	theme	vein	1003:1006	arg1	phenotype					1008:1016	a wing vein phenotype	996:1016	a wing vein phenotype	996:1016	We observed that reduced total protein O-GlcNAcylation in the sxc mutant is associated with a wing vein phenotype and temperature-dependent lethality.
29588363	7	23	theme	scutellar	1233:1241	arg1	numbers					1251:1257	abnormal scutellar bristle numbers	1224:1257	abnormal scutellar bristle numbers	1224:1257	Genetic interaction between sxc and a null allele of Drosophila host cell factor (dHcf), encoding an extensively O-GlcNAcylated transcriptional coactivator, resulted in abnormal scutellar bristle numbers.
29588363	1	24	theme	regulatory	196:205	arg1	mechanism					207:215	an essential regulatory mechanism	183:215	an essential regulatory mechanism in many cellular processes	183:242	Post-translational modification of serine/threonine residues in nucleocytoplasmic proteins with GlcNAc (O-GlcNAcylation) is an essential regulatory mechanism in many cellular processes.
29588363	1	24	theme	regulatory	196:205	arg1	modification					78:89	Post-translational modification	59:89	Post-translational modification of serine/threonine residues in nucleocytoplasmic proteins with GlcNAc (O-GlcNAcylation)	59:178	Post-translational modification of serine/threonine residues in nucleocytoplasmic proteins with GlcNAc (O-GlcNAcylation) is an essential regulatory mechanism in many cellular processes.
29588363	0	25	from	Effects	0:6	arg1	development					46:56	Drosophila development	35:56	Drosophila development	35:56	Effects of hypo-O-GlcNAcylation on Drosophila development.
29588363	3	26	theme	Drosophila	440:449	arg1	development					451:461	Drosophila development	440:461	Drosophila development	440:461	To dissect the requirement for O-GlcNAc signaling in Drosophila development, we used CRISPR/Cas9 gene editing to generate rationally designed sxc catalytically hypomorphic or null point mutants.
29588363	1	27	theme	residues	111:118	arg1	modification					78:89	Post-translational modification	59:89	Post-translational modification of serine/threonine residues in nucleocytoplasmic proteins with GlcNAc (O-GlcNAcylation)	59:178	Post-translational modification of serine/threonine residues in nucleocytoplasmic proteins with GlcNAc (O-GlcNAcylation) is an essential regulatory mechanism in many cellular processes.
29588363	1	27	theme	residues	111:118	arg1	mechanism					207:215	an essential regulatory mechanism	183:215	an essential regulatory mechanism in many cellular processes	183:242	Post-translational modification of serine/threonine residues in nucleocytoplasmic proteins with GlcNAc (O-GlcNAcylation) is an essential regulatory mechanism in many cellular processes.
29588363	5	28	theme	mutants	770:776	arg1	One					757:759	One	757:759	One	757:759	One of these mutants, the catalytically inactive sxc , was recessive lethal, whereas a second mutant, the hypomorphic sxc , was homozygous viable.
29588363	5	28	theme	mutants	770:776	arg1	mutants					770:776	these mutants	764:776	these mutants	764:776	One of these mutants, the catalytically inactive sxc , was recessive lethal, whereas a second mutant, the hypomorphic sxc , was homozygous viable.
29588363	5	28	theme	mutants	770:776	arg1	sxc					806:808	the catalytically inactive sxc	779:808	the catalytically inactive sxc	779:808	One of these mutants, the catalytically inactive sxc , was recessive lethal, whereas a second mutant, the hypomorphic sxc , was homozygous viable.
29588363	8	29	theme	complex	1353:1359	arg1	skuld					1329:1333	skuld	1329:1333	skuld (skd)	1329:1339	A similar phenotype was also observed in sxc flies lacking a copy of skuld (skd), a Mediator complex gene known to affect scutellar bristle formation.
29588363	8	29	theme	complex	1353:1359	arg1	gene					1361:1364	a Mediator complex gene	1342:1364	a Mediator complex gene known to affect scutellar bristle formation	1342:1408	A similar phenotype was also observed in sxc flies lacking a copy of skuld (skd), a Mediator complex gene known to affect scutellar bristle formation.
29588363	10	30	theme	reduced	1640:1646	arg1	O-GlcNAc					1656:1663	globally reduced protein O-GlcNAc	1631:1663	globally reduced protein O-GlcNAc	1631:1663	In conclusion, the generation of the endogenous OGT hypomorphic mutant sxc enabled us to identify pleiotropic effects of globally reduced protein O-GlcNAc during Drosophila development.
29588363	7	31	theme	transcriptional	1183:1197	arg1	coactivator					1199:1209	an extensively O-GlcNAcylated transcriptional coactivator	1153:1209	an extensively O-GlcNAcylated transcriptional coactivator	1153:1209	Genetic interaction between sxc and a null allele of Drosophila host cell factor (dHcf), encoding an extensively O-GlcNAcylated transcriptional coactivator, resulted in abnormal scutellar bristle numbers.
29588363	1	32	theme	nucleocytoplasmic	123:139	arg1	proteins					141:148	nucleocytoplasmic proteins	123:148	nucleocytoplasmic proteins with GlcNAc (O-GlcNAcylation)	123:178	Post-translational modification of serine/threonine residues in nucleocytoplasmic proteins with GlcNAc (O-GlcNAcylation) is an essential regulatory mechanism in many cellular processes.
29588363	2	33	theme	Polycomb	280:287	arg1	gene					289:292	the Polycomb gene O-GlcNAc transferase (OGT; also known as super sex combs (sxc))	276:356	the Polycomb gene O-GlcNAc transferase (OGT; also known as super sex combs (sxc))	276:356	In Drosophila, null mutants of the Polycomb gene O-GlcNAc transferase (OGT; also known as super sex combs (sxc)) display homeotic phenotypes.
29588363	0	34	theme	hypo-O-GlcNAcylation	11:30	arg1	Effects					0:6	Effects	0:6	Effects of hypo-O-GlcNAcylation on Drosophila development	0:56	Effects of hypo-O-GlcNAcylation on Drosophila development.
29588363	10	35	theme	Drosophila	1672:1681	arg1	development					1683:1693	Drosophila development	1672:1693	Drosophila development	1672:1693	In conclusion, the generation of the endogenous OGT hypomorphic mutant sxc enabled us to identify pleiotropic effects of globally reduced protein O-GlcNAc during Drosophila development.
29588363	0	36	theme	Drosophila	35:44	arg1	development					46:56	Drosophila development	35:56	Drosophila development	35:56	Effects of hypo-O-GlcNAcylation on Drosophila development.
29588363	8	37	theme	bristle	1392:1398	arg1	formation					1400:1408	scutellar bristle formation	1382:1408	scutellar bristle formation	1382:1408	A similar phenotype was also observed in sxc flies lacking a copy of skuld (skd), a Mediator complex gene known to affect scutellar bristle formation.
29588363	4	38	contain	carrying	685:692	arg1	progeny					677:683	progeny	677:683	progeny carrying precise point mutations with no detectable off-target effects	677:754	Of the fertile males derived from embryos injected with the CRISPR/Cas9 reagents, 25% produced progeny carrying precise point mutations with no detectable off-target effects.
29588363	4	38	contain	carrying	685:692	arg2	mutations					708:716	precise point mutations	694:716	precise point mutations	694:716	Of the fertile males derived from embryos injected with the CRISPR/Cas9 reagents, 25% produced progeny carrying precise point mutations with no detectable off-target effects.
29588363	3	39	theme	gene	484:487	arg1	editing					489:495	CRISPR/Cas9 gene editing	472:495	CRISPR/Cas9 gene editing	472:495	To dissect the requirement for O-GlcNAc signaling in Drosophila development, we used CRISPR/Cas9 gene editing to generate rationally designed sxc catalytically hypomorphic or null point mutants.
29588363	2	40	dep	transferase	303:313	arg1	known					326:330	known	326:330	known as super sex combs (sxc)	326:355	In Drosophila, null mutants of the Polycomb gene O-GlcNAc transferase (OGT; also known as super sex combs (sxc)) display homeotic phenotypes.
29588363	2	41	theme	homeotic	366:373	arg1	phenotypes					375:384	homeotic phenotypes	366:384	homeotic phenotypes	366:384	In Drosophila, null mutants of the Polycomb gene O-GlcNAc transferase (OGT; also known as super sex combs (sxc)) display homeotic phenotypes.
29588363	6	42	theme	reduced	921:927	arg1	O-GlcNAcylation					943:957	reduced total protein O-GlcNAcylation	921:957	reduced total protein O-GlcNAcylation in the sxc mutant	921:975	We observed that reduced total protein O-GlcNAcylation in the sxc mutant is associated with a wing vein phenotype and temperature-dependent lethality.
29588363	1	43	theme	Post-translational	59:76	arg1	modification					78:89	Post-translational modification	59:89	Post-translational modification of serine/threonine residues in nucleocytoplasmic proteins with GlcNAc (O-GlcNAcylation)	59:178	Post-translational modification of serine/threonine residues in nucleocytoplasmic proteins with GlcNAc (O-GlcNAcylation) is an essential regulatory mechanism in many cellular processes.
29588363	1	43	theme	Post-translational	59:76	arg1	mechanism					207:215	an essential regulatory mechanism	183:215	an essential regulatory mechanism in many cellular processes	183:242	Post-translational modification of serine/threonine residues in nucleocytoplasmic proteins with GlcNAc (O-GlcNAcylation) is an essential regulatory mechanism in many cellular processes.
29588363	7	44	theme	factor	1129:1134	arg1	sxc					1083:1085	sxc	1083:1085	sxc	1083:1085	Genetic interaction between sxc and a null allele of Drosophila host cell factor (dHcf), encoding an extensively O-GlcNAcylated transcriptional coactivator, resulted in abnormal scutellar bristle numbers.
29588363	7	44	theme	factor	1129:1134	arg1	allele					1098:1103	a null allele	1091:1103	a null allele of Drosophila host cell factor (dHcf)	1091:1141	Genetic interaction between sxc and a null allele of Drosophila host cell factor (dHcf), encoding an extensively O-GlcNAcylated transcriptional coactivator, resulted in abnormal scutellar bristle numbers.
29588363	4	45	theme	fertile	589:595	arg1	males					597:601	the fertile males	585:601	the fertile males derived from embryos injected with the CRISPR/Cas9 reagents	585:661	Of the fertile males derived from embryos injected with the CRISPR/Cas9 reagents, 25% produced progeny carrying precise point mutations with no detectable off-target effects.
29588363	10	46	theme	mutant	1574:1579	arg1	sxc					1581:1583	the endogenous OGT hypomorphic mutant sxc	1543:1583	the endogenous OGT hypomorphic mutant sxc	1543:1583	In conclusion, the generation of the endogenous OGT hypomorphic mutant sxc enabled us to identify pleiotropic effects of globally reduced protein O-GlcNAc during Drosophila development.
29588363	7	47	theme	host	1119:1122	arg1	dHcf					1137:1140	dHcf	1137:1140	dHcf	1137:1140	Genetic interaction between sxc and a null allele of Drosophila host cell factor (dHcf), encoding an extensively O-GlcNAcylated transcriptional coactivator, resulted in abnormal scutellar bristle numbers.
29588363	7	47	theme	host	1119:1122	arg1	factor					1129:1134	Drosophila host cell factor	1108:1134	Drosophila host cell factor (dHcf)	1108:1141	Genetic interaction between sxc and a null allele of Drosophila host cell factor (dHcf), encoding an extensively O-GlcNAcylated transcriptional coactivator, resulted in abnormal scutellar bristle numbers.
29588363	3	48	theme	null	562:565	arg1	mutants					573:579	null point mutants	562:579	null point mutants	562:579	To dissect the requirement for O-GlcNAc signaling in Drosophila development, we used CRISPR/Cas9 gene editing to generate rationally designed sxc catalytically hypomorphic or null point mutants.
29588363	10	49	theme	OGT	1558:1560	arg1	sxc					1581:1583	the endogenous OGT hypomorphic mutant sxc	1543:1583	the endogenous OGT hypomorphic mutant sxc	1543:1583	In conclusion, the generation of the endogenous OGT hypomorphic mutant sxc enabled us to identify pleiotropic effects of globally reduced protein O-GlcNAc during Drosophila development.
29588363	3	50	theme	hypomorphic	547:557	arg1	sxc					529:531	rationally designed sxc	509:531	rationally designed sxc catalytically hypomorphic	509:557	To dissect the requirement for O-GlcNAc signaling in Drosophila development, we used CRISPR/Cas9 gene editing to generate rationally designed sxc catalytically hypomorphic or null point mutants.
29588363	7	51	theme	null	1093:1096	arg1	allele					1098:1103	a null allele	1091:1103	a null allele of Drosophila host cell factor (dHcf)	1091:1141	Genetic interaction between sxc and a null allele of Drosophila host cell factor (dHcf), encoding an extensively O-GlcNAcylated transcriptional coactivator, resulted in abnormal scutellar bristle numbers.
29588363	2	52	theme	super	335:339	arg1	sxc					352:354	sxc	352:354	sxc	352:354	In Drosophila, null mutants of the Polycomb gene O-GlcNAc transferase (OGT; also known as super sex combs (sxc)) display homeotic phenotypes.
29588363	2	52	theme	super	335:339	arg1	combs					345:349	super sex combs	335:349	super sex combs (sxc)	335:355	In Drosophila, null mutants of the Polycomb gene O-GlcNAc transferase (OGT; also known as super sex combs (sxc)) display homeotic phenotypes.
29588363	1	53	theme	many	220:223	arg1	processes					234:242	many cellular processes	220:242	many cellular processes	220:242	Post-translational modification of serine/threonine residues in nucleocytoplasmic proteins with GlcNAc (O-GlcNAcylation) is an essential regulatory mechanism in many cellular processes.
29588363	2	54	dep	gene	289:292	arg1	transferase					303:313	O-GlcNAc transferase	294:313	the Polycomb gene O-GlcNAc transferase (OGT; also known as super sex combs (sxc))	276:356	In Drosophila, null mutants of the Polycomb gene O-GlcNAc transferase (OGT; also known as super sex combs (sxc)) display homeotic phenotypes.
29588363	6	55	theme	sxc	966:968	arg1	mutant					970:975	the sxc mutant	962:975	the sxc mutant	962:975	We observed that reduced total protein O-GlcNAcylation in the sxc mutant is associated with a wing vein phenotype and temperature-dependent lethality.
29588363	4	56	theme	CRISPR/Cas9	642:652	arg1	reagents					654:661	the CRISPR/Cas9 reagents	638:661	the CRISPR/Cas9 reagents	638:661	Of the fertile males derived from embryos injected with the CRISPR/Cas9 reagents, 25% produced progeny carrying precise point mutations with no detectable off-target effects.
29588363	8	57	theme	similar	1262:1268	arg1	phenotype					1270:1278	A similar phenotype	1260:1278	A similar phenotype	1260:1278	A similar phenotype was also observed in sxc flies lacking a copy of skuld (skd), a Mediator complex gene known to affect scutellar bristle formation.
29588363	9	58	theme	Polycomb	1464:1471	arg1	function					1473:1480	OGT Polycomb function	1460:1480	OGT Polycomb function	1460:1480	Interestingly, this phenotype was independent of OGT Polycomb function or dHcf downstream targets.
29588363	10	59	theme	pleiotropic	1608:1618	arg1	effects					1620:1626	pleiotropic effects	1608:1626	pleiotropic effects of globally reduced protein O-GlcNAc during Drosophila development	1608:1693	In conclusion, the generation of the endogenous OGT hypomorphic mutant sxc enabled us to identify pleiotropic effects of globally reduced protein O-GlcNAc during Drosophila development.
29588363	7	60	theme	Genetic	1055:1061	arg1	interaction					1063:1073	Genetic interaction	1055:1073	Genetic interaction	1055:1073	Genetic interaction between sxc and a null allele of Drosophila host cell factor (dHcf), encoding an extensively O-GlcNAcylated transcriptional coactivator, resulted in abnormal scutellar bristle numbers.
29588363	2	61	theme	null	260:263	arg1	mutants					265:271	null mutants	260:271	null mutants of the Polycomb gene O-GlcNAc transferase (OGT; also known as super sex combs (sxc))	260:356	In Drosophila, null mutants of the Polycomb gene O-GlcNAc transferase (OGT; also known as super sex combs (sxc)) display homeotic phenotypes.
29588363	6	62	theme	protein	935:941	arg1	O-GlcNAcylation					943:957	reduced total protein O-GlcNAcylation	921:957	reduced total protein O-GlcNAcylation in the sxc mutant	921:975	We observed that reduced total protein O-GlcNAcylation in the sxc mutant is associated with a wing vein phenotype and temperature-dependent lethality.
29588363	8	63	theme	sxc	1301:1303	arg1	flies					1305:1309	sxc flies	1301:1309	sxc flies lacking a copy of skuld (skd), a Mediator complex gene known to affect scutellar bristle formation	1301:1408	A similar phenotype was also observed in sxc flies lacking a copy of skuld (skd), a Mediator complex gene known to affect scutellar bristle formation.
29588363	11	64	theme	Drosophila	1829:1838	arg1	development					1840:1850	Drosophila development	1829:1850	Drosophila development	1829:1850	The mutants generated and phenotypes observed in this study provide a platform for discovery of OGT substrates that are critical for Drosophila development.
29588363	2	65	theme	O-GlcNAc	294:301	arg1	transferase					303:313	O-GlcNAc transferase	294:313	the Polycomb gene O-GlcNAc transferase (OGT; also known as super sex combs (sxc))	276:356	In Drosophila, null mutants of the Polycomb gene O-GlcNAc transferase (OGT; also known as super sex combs (sxc)) display homeotic phenotypes.
29588363	9	66	theme	downstream	1490:1499	arg1	targets					1501:1507	dHcf downstream targets	1485:1507	dHcf downstream targets	1485:1507	Interestingly, this phenotype was independent of OGT Polycomb function or dHcf downstream targets.
29588363	6	67	theme	temperature-dependent	1022:1042	arg1	lethality					1044:1052	temperature-dependent lethality	1022:1052	temperature-dependent lethality	1022:1052	We observed that reduced total protein O-GlcNAcylation in the sxc mutant is associated with a wing vein phenotype and temperature-dependent lethality.
29588363	4	68	theme	off-target	737:746	arg1	effects					748:754	no detectable off-target effects	723:754	no detectable off-target effects	723:754	Of the fertile males derived from embryos injected with the CRISPR/Cas9 reagents, 25% produced progeny carrying precise point mutations with no detectable off-target effects.
29588363	7	69	theme	bristle	1243:1249	arg1	numbers					1251:1257	abnormal scutellar bristle numbers	1224:1257	abnormal scutellar bristle numbers	1224:1257	Genetic interaction between sxc and a null allele of Drosophila host cell factor (dHcf), encoding an extensively O-GlcNAcylated transcriptional coactivator, resulted in abnormal scutellar bristle numbers.
29588363	4	70	attach	derived	603:609	arg1	embryos					616:622	embryos	616:622	embryos injected with the CRISPR/Cas9 reagents	616:661	Of the fertile males derived from embryos injected with the CRISPR/Cas9 reagents, 25% produced progeny carrying precise point mutations with no detectable off-target effects.
29588363	4	70	attach	derived	603:609	arg2	males					597:601	the fertile males	585:601	the fertile males derived from embryos injected with the CRISPR/Cas9 reagents	585:661	Of the fertile males derived from embryos injected with the CRISPR/Cas9 reagents, 25% produced progeny carrying precise point mutations with no detectable off-target effects.
29588363	4	71	theme	precise	694:700	arg1	mutations					708:716	precise point mutations	694:716	precise point mutations	694:716	Of the fertile males derived from embryos injected with the CRISPR/Cas9 reagents, 25% produced progeny carrying precise point mutations with no detectable off-target effects.
29588363	7	72	theme	abnormal	1224:1231	arg1	numbers					1251:1257	abnormal scutellar bristle numbers	1224:1257	abnormal scutellar bristle numbers	1224:1257	Genetic interaction between sxc and a null allele of Drosophila host cell factor (dHcf), encoding an extensively O-GlcNAcylated transcriptional coactivator, resulted in abnormal scutellar bristle numbers.
29588363	3	73	from	requirement	402:412	arg1	development					451:461	Drosophila development	440:461	Drosophila development	440:461	To dissect the requirement for O-GlcNAc signaling in Drosophila development, we used CRISPR/Cas9 gene editing to generate rationally designed sxc catalytically hypomorphic or null point mutants.
29588363	1	74	theme	essential	186:194	arg1	mechanism					207:215	an essential regulatory mechanism	183:215	an essential regulatory mechanism in many cellular processes	183:242	Post-translational modification of serine/threonine residues in nucleocytoplasmic proteins with GlcNAc (O-GlcNAcylation) is an essential regulatory mechanism in many cellular processes.
29588363	1	74	theme	essential	186:194	arg1	modification					78:89	Post-translational modification	59:89	Post-translational modification of serine/threonine residues in nucleocytoplasmic proteins with GlcNAc (O-GlcNAcylation)	59:178	Post-translational modification of serine/threonine residues in nucleocytoplasmic proteins with GlcNAc (O-GlcNAcylation) is an essential regulatory mechanism in many cellular processes.
29588363	6	75	theme	wing	998:1001	arg1	phenotype					1008:1016	a wing vein phenotype	996:1016	a wing vein phenotype	996:1016	We observed that reduced total protein O-GlcNAcylation in the sxc mutant is associated with a wing vein phenotype and temperature-dependent lethality.
29588363	10	76	theme	O-GlcNAc	1656:1663	arg1	effects					1620:1626	pleiotropic effects	1608:1626	pleiotropic effects of globally reduced protein O-GlcNAc during Drosophila development	1608:1693	In conclusion, the generation of the endogenous OGT hypomorphic mutant sxc enabled us to identify pleiotropic effects of globally reduced protein O-GlcNAc during Drosophila development.
29588363	8	77	theme	skuld	1329:1333	arg1	copy					1321:1324	a copy	1319:1324	a copy of skuld (skd), a Mediator complex gene known to affect scutellar bristle formation	1319:1408	A similar phenotype was also observed in sxc flies lacking a copy of skuld (skd), a Mediator complex gene known to affect scutellar bristle formation.
29588363	11	78	theme	OGT	1792:1794	arg1	substrates					1796:1805	OGT substrates	1792:1805	OGT substrates that are critical for Drosophila development	1792:1850	The mutants generated and phenotypes observed in this study provide a platform for discovery of OGT substrates that are critical for Drosophila development.
29588363	9	79	theme	OGT	1460:1462	arg1	function					1473:1480	OGT Polycomb function	1460:1480	OGT Polycomb function	1460:1480	Interestingly, this phenotype was independent of OGT Polycomb function or dHcf downstream targets.
29588363	1	80	theme	serine/threonine	94:109	arg1	residues					111:118	serine/threonine residues	94:118	serine/threonine residues	94:118	Post-translational modification of serine/threonine residues in nucleocytoplasmic proteins with GlcNAc (O-GlcNAcylation) is an essential regulatory mechanism in many cellular processes.
29588363	11	81	located	observed	1733:1740	arg1	study					1750:1754	this study	1745:1754	this study	1745:1754	The mutants generated and phenotypes observed in this study provide a platform for discovery of OGT substrates that are critical for Drosophila development.
29588363	11	81	located	observed	1733:1740	arg2	phenotypes					1722:1731	phenotypes	1722:1731	phenotypes observed in this study	1722:1754	The mutants generated and phenotypes observed in this study provide a platform for discovery of OGT substrates that are critical for Drosophila development.
29588363	10	82	theme	protein	1648:1654	arg1	O-GlcNAc					1656:1663	globally reduced protein O-GlcNAc	1631:1663	globally reduced protein O-GlcNAc	1631:1663	In conclusion, the generation of the endogenous OGT hypomorphic mutant sxc enabled us to identify pleiotropic effects of globally reduced protein O-GlcNAc during Drosophila development.
29588363	7	83	theme	O-GlcNAcylated	1168:1181	arg1	coactivator					1199:1209	an extensively O-GlcNAcylated transcriptional coactivator	1153:1209	an extensively O-GlcNAcylated transcriptional coactivator	1153:1209	Genetic interaction between sxc and a null allele of Drosophila host cell factor (dHcf), encoding an extensively O-GlcNAcylated transcriptional coactivator, resulted in abnormal scutellar bristle numbers.
29588363	8	84	located	observed	1289:1296	arg2	phenotype					1270:1278	A similar phenotype	1260:1278	A similar phenotype	1260:1278	A similar phenotype was also observed in sxc flies lacking a copy of skuld (skd), a Mediator complex gene known to affect scutellar bristle formation.
29588363	8	84	located	observed	1289:1296	arg1	flies					1305:1309	sxc flies	1301:1309	sxc flies lacking a copy of skuld (skd), a Mediator complex gene known to affect scutellar bristle formation	1301:1408	A similar phenotype was also observed in sxc flies lacking a copy of skuld (skd), a Mediator complex gene known to affect scutellar bristle formation.
29588363	8	85	theme	Mediator	1344:1351	arg1	skuld					1329:1333	skuld	1329:1333	skuld (skd)	1329:1339	A similar phenotype was also observed in sxc flies lacking a copy of skuld (skd), a Mediator complex gene known to affect scutellar bristle formation.
29588363	8	85	theme	Mediator	1344:1351	arg1	gene					1361:1364	a Mediator complex gene	1342:1364	a Mediator complex gene known to affect scutellar bristle formation	1342:1408	A similar phenotype was also observed in sxc flies lacking a copy of skuld (skd), a Mediator complex gene known to affect scutellar bristle formation.
29588363	3	86	used	used	467:470	arg2	we					464:465	we	464:465	we	464:465	To dissect the requirement for O-GlcNAc signaling in Drosophila development, we used CRISPR/Cas9 gene editing to generate rationally designed sxc catalytically hypomorphic or null point mutants.
29588363	3	87	theme	CRISPR/Cas9	472:482	arg1	editing					489:495	CRISPR/Cas9 gene editing	472:495	CRISPR/Cas9 gene editing	472:495	To dissect the requirement for O-GlcNAc signaling in Drosophila development, we used CRISPR/Cas9 gene editing to generate rationally designed sxc catalytically hypomorphic or null point mutants.
29588363	5	88	theme	inactive	797:804	arg1	sxc					806:808	the catalytically inactive sxc	779:808	the catalytically inactive sxc	779:808	One of these mutants, the catalytically inactive sxc , was recessive lethal, whereas a second mutant, the hypomorphic sxc , was homozygous viable.
29588363	5	88	theme	inactive	797:804	arg1	mutants					770:776	these mutants	764:776	these mutants	764:776	One of these mutants, the catalytically inactive sxc , was recessive lethal, whereas a second mutant, the hypomorphic sxc , was homozygous viable.
29588363	5	88	theme	inactive	797:804	arg1	One					757:759	One	757:759	One	757:759	One of these mutants, the catalytically inactive sxc , was recessive lethal, whereas a second mutant, the hypomorphic sxc , was homozygous viable.
29588363	3	89	theme	O-GlcNAc	418:425	arg1	signaling					427:435	O-GlcNAc signaling	418:435	O-GlcNAc signaling	418:435	To dissect the requirement for O-GlcNAc signaling in Drosophila development, we used CRISPR/Cas9 gene editing to generate rationally designed sxc catalytically hypomorphic or null point mutants.
29588363	8	90	theme	scutellar	1382:1390	arg1	formation					1400:1408	scutellar bristle formation	1382:1408	scutellar bristle formation	1382:1408	A similar phenotype was also observed in sxc flies lacking a copy of skuld (skd), a Mediator complex gene known to affect scutellar bristle formation.
29588363	6	91	from	O-GlcNAcylation	943:957	arg1	mutant					970:975	the sxc mutant	962:975	the sxc mutant	962:975	We observed that reduced total protein O-GlcNAcylation in the sxc mutant is associated with a wing vein phenotype and temperature-dependent lethality.
29588363	2	92	theme	sex	341:343	arg1	sxc					352:354	sxc	352:354	sxc	352:354	In Drosophila, null mutants of the Polycomb gene O-GlcNAc transferase (OGT; also known as super sex combs (sxc)) display homeotic phenotypes.
29588363	2	92	theme	sex	341:343	arg1	combs					345:349	super sex combs	335:349	super sex combs (sxc)	335:355	In Drosophila, null mutants of the Polycomb gene O-GlcNAc transferase (OGT; also known as super sex combs (sxc)) display homeotic phenotypes.
31849975	4	0	link	cell-derived	609:620	arg1	macrophages					622:632	THP-1 cell-derived macrophages	603:632	THP-1 cell-derived macrophages	603:632	THP-1 cell-derived macrophages were cultured on JDBM, Ti-6Al-4V alloy (Ti), 15% extract of JDBM, and 7.5 mM of MgCl2 for 1 h before the addition of LPS for an indicated time; the experiments included negative and positive controls.
31849975	11	1	theme	signaling	1972:1980	arg1	advantage					2016:2024	a potential advantage	2004:2024	a potential advantage	2004:2024	Together, our results demonstrated the degradation products of Mg-based alloy, especially magnesium, and resolved inflammation by activation of the TRPM7-PI3K-AKT1 signaling pathway, which may be a potential advantage or target to promote biodegradable Mg-based alloy applications.
31849975	11	1	theme	signaling	1972:1980	arg1	pathway					1982:1988	the TRPM7-PI3K-AKT1 signaling pathway	1952:1988	the TRPM7-PI3K-AKT1 signaling pathway	1952:1988	Together, our results demonstrated the degradation products of Mg-based alloy, especially magnesium, and resolved inflammation by activation of the TRPM7-PI3K-AKT1 signaling pathway, which may be a potential advantage or target to promote biodegradable Mg-based alloy applications.
31849975	2	2	theme	signaling	364:372	arg1	mechanism					374:382	the precise signaling mechanism	352:382	the precise signaling mechanism underlying the inhibition of inflammation by Mg-based alloys	352:443	However, the precise signaling mechanism underlying the inhibition of inflammation by Mg-based alloys has not been elucidated.
31849975	11	3	theme	alloy	1880:1884	arg1	products					1859:1866	the degradation products	1843:1866	the degradation products of Mg-based alloy, especially magnesium, and resolved inflammation	1843:1933	Together, our results demonstrated the degradation products of Mg-based alloy, especially magnesium, and resolved inflammation by activation of the TRPM7-PI3K-AKT1 signaling pathway, which may be a potential advantage or target to promote biodegradable Mg-based alloy applications.
31849975	9	4	theme	knocking	1567:1574	arg1	TRPM7					1581:1585	knocking down TRPM7	1567:1585	knocking down TRPM7 of macrophages	1567:1600	Interestingly, the expression of LPS-induced TNF and IL-6 could be recovered by knocking down TRPM7 of macrophages, in the presence of extract or MgCl2.
31849975	1	5	theme	clinical	192:199	arg1	applications					201:212	clinical applications	192:212	clinical applications owing to favorable mechanical properties, biodegradability, biocompatibility, and especially their anti-inflammatory properties	192:340	Mg-based alloys might be ideal biomaterials in clinical applications owing to favorable mechanical properties, biodegradability, biocompatibility, and especially their anti-inflammatory properties.
31849975	4	6	theme	JDBM	694:697	arg1	extract					683:689	15% extract	679:689	15% extract of JDBM	679:697	THP-1 cell-derived macrophages were cultured on JDBM, Ti-6Al-4V alloy (Ti), 15% extract of JDBM, and 7.5 mM of MgCl2 for 1 h before the addition of LPS for an indicated time; the experiments included negative and positive controls.
31849975	4	6	theme	JDBM	694:697	arg1	Ti					674:675	Ti	674:675	Ti	674:675	THP-1 cell-derived macrophages were cultured on JDBM, Ti-6Al-4V alloy (Ti), 15% extract of JDBM, and 7.5 mM of MgCl2 for 1 h before the addition of LPS for an indicated time; the experiments included negative and positive controls.
31849975	4	6	theme	JDBM	694:697	arg1	JDBM					651:654	JDBM	651:654	JDBM	651:654	THP-1 cell-derived macrophages were cultured on JDBM, Ti-6Al-4V alloy (Ti), 15% extract of JDBM, and 7.5 mM of MgCl2 for 1 h before the addition of LPS for an indicated time; the experiments included negative and positive controls.
31849975	4	6	theme	JDBM	694:697	arg1	alloy					667:671	Ti-6Al-4V alloy	657:671	Ti-6Al-4V alloy (Ti)	657:676	THP-1 cell-derived macrophages were cultured on JDBM, Ti-6Al-4V alloy (Ti), 15% extract of JDBM, and 7.5 mM of MgCl2 for 1 h before the addition of LPS for an indicated time; the experiments included negative and positive controls.
31849975	4	6	theme	JDBM	694:697	arg1	mM					708:709	7.5 mM	704:709	7.5 mM of MgCl2	704:718	THP-1 cell-derived macrophages were cultured on JDBM, Ti-6Al-4V alloy (Ti), 15% extract of JDBM, and 7.5 mM of MgCl2 for 1 h before the addition of LPS for an indicated time; the experiments included negative and positive controls.
31849975	2	7	theme	precise	356:362	arg1	mechanism					374:382	the precise signaling mechanism	352:382	the precise signaling mechanism underlying the inhibition of inflammation by Mg-based alloys	352:443	However, the precise signaling mechanism underlying the inhibition of inflammation by Mg-based alloys has not been elucidated.
31849975	9	8	theme	macrophages	1590:1600	arg1	TRPM7					1581:1585	knocking down TRPM7	1567:1585	knocking down TRPM7 of macrophages	1567:1600	Interestingly, the expression of LPS-induced TNF and IL-6 could be recovered by knocking down TRPM7 of macrophages, in the presence of extract or MgCl2.
31849975	1	9	theme	favorable	223:231	arg1	properties					244:253	favorable mechanical properties	223:253	favorable mechanical properties	223:253	Mg-based alloys might be ideal biomaterials in clinical applications owing to favorable mechanical properties, biodegradability, biocompatibility, and especially their anti-inflammatory properties.
31849975	4	10	theme	15	679:680	arg1	%					681:681	%	681:681	%	681:681	THP-1 cell-derived macrophages were cultured on JDBM, Ti-6Al-4V alloy (Ti), 15% extract of JDBM, and 7.5 mM of MgCl2 for 1 h before the addition of LPS for an indicated time; the experiments included negative and positive controls.
31849975	4	11	theme	MgCl2	714:718	arg1	extract					683:689	15% extract	679:689	15% extract of JDBM	679:697	THP-1 cell-derived macrophages were cultured on JDBM, Ti-6Al-4V alloy (Ti), 15% extract of JDBM, and 7.5 mM of MgCl2 for 1 h before the addition of LPS for an indicated time; the experiments included negative and positive controls.
31849975	4	11	theme	MgCl2	714:718	arg1	Ti					674:675	Ti	674:675	Ti	674:675	THP-1 cell-derived macrophages were cultured on JDBM, Ti-6Al-4V alloy (Ti), 15% extract of JDBM, and 7.5 mM of MgCl2 for 1 h before the addition of LPS for an indicated time; the experiments included negative and positive controls.
31849975	4	11	theme	MgCl2	714:718	arg1	JDBM					651:654	JDBM	651:654	JDBM	651:654	THP-1 cell-derived macrophages were cultured on JDBM, Ti-6Al-4V alloy (Ti), 15% extract of JDBM, and 7.5 mM of MgCl2 for 1 h before the addition of LPS for an indicated time; the experiments included negative and positive controls.
31849975	4	11	theme	MgCl2	714:718	arg1	alloy					667:671	Ti-6Al-4V alloy	657:671	Ti-6Al-4V alloy (Ti)	657:676	THP-1 cell-derived macrophages were cultured on JDBM, Ti-6Al-4V alloy (Ti), 15% extract of JDBM, and 7.5 mM of MgCl2 for 1 h before the addition of LPS for an indicated time; the experiments included negative and positive controls.
31849975	4	11	theme	MgCl2	714:718	arg1	mM					708:709	7.5 mM	704:709	7.5 mM of MgCl2	704:718	THP-1 cell-derived macrophages were cultured on JDBM, Ti-6Al-4V alloy (Ti), 15% extract of JDBM, and 7.5 mM of MgCl2 for 1 h before the addition of LPS for an indicated time; the experiments included negative and positive controls.
31849975	9	12	theme	extract	1622:1628	arg1	presence					1610:1617	the presence	1606:1617	the presence of extract or MgCl2	1606:1637	Interestingly, the expression of LPS-induced TNF and IL-6 could be recovered by knocking down TRPM7 of macrophages, in the presence of extract or MgCl2.
31849975	11	13	theme	Mg-based	1871:1878	arg1	alloy					1880:1884	Mg-based alloy	1871:1884	Mg-based alloy	1871:1884	Together, our results demonstrated the degradation products of Mg-based alloy, especially magnesium, and resolved inflammation by activation of the TRPM7-PI3K-AKT1 signaling pathway, which may be a potential advantage or target to promote biodegradable Mg-based alloy applications.
31849975	5	14	theme	tumor	906:910	arg1	TNF					929:931	TNF	929:931	TNF	929:931	Our results showed JDBM, extract, and MgCl2 could decrease LPS-induced tumor necrosis factor (TNF) and interleukin (IL)-6 expression.
31849975	5	14	theme	tumor	906:910	arg1	factor					921:926	tumor necrosis factor	906:926	tumor necrosis factor (TNF)	906:932	Our results showed JDBM, extract, and MgCl2 could decrease LPS-induced tumor necrosis factor (TNF) and interleukin (IL)-6 expression.
31849975	5	15	theme	LPS-induced	894:904	arg1	expression					957:966	LPS-induced tumor necrosis factor (TNF) and interleukin (IL)-6 expression	894:966	LPS-induced tumor necrosis factor (TNF) and interleukin (IL)-6 expression	894:966	Our results showed JDBM, extract, and MgCl2 could decrease LPS-induced tumor necrosis factor (TNF) and interleukin (IL)-6 expression.
31849975	9	16	theme	MgCl2	1633:1637	arg1	presence					1610:1617	the presence	1606:1617	the presence of extract or MgCl2	1606:1637	Interestingly, the expression of LPS-induced TNF and IL-6 could be recovered by knocking down TRPM7 of macrophages, in the presence of extract or MgCl2.
31849975	7	17	theme	positive	1161:1168	arg1	control					1170:1176	the positive control	1157:1176	the positive control	1157:1176	Mechanically, extract and MgCl2 downregulated the expression of toll-like receptor (TLR)-4 and MYD88 compared with the positive control and inhibited LPS-induced nuclear factor-kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways by inactivation of the phosphorylation of IKK-α/β, IKβ-α, P65, P38, and JNK.
31849975	7	18	theme	receptor	1116:1123	arg1	expression					1092:1101	the expression	1088:1101	the expression of toll-like receptor (TLR)-4 and MYD88	1088:1141	Mechanically, extract and MgCl2 downregulated the expression of toll-like receptor (TLR)-4 and MYD88 compared with the positive control and inhibited LPS-induced nuclear factor-kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways by inactivation of the phosphorylation of IKK-α/β, IKβ-α, P65, P38, and JNK.
31849975	8	19	theme	reactive	1405:1412	arg1	species					1421:1427	reactive oxygen species	1405:1427	the LPS-induced reactive oxygen species (ROS) expression	1389:1444	Additionally, the LPS-induced reactive oxygen species (ROS) expression was also decreased by extract and MgCl2.
31849975	8	19	theme	reactive	1405:1412	arg1	ROS					1430:1432	ROS	1430:1432	ROS	1430:1432	Additionally, the LPS-induced reactive oxygen species (ROS) expression was also decreased by extract and MgCl2.
31849975	0	20	theme	Cell-Derived	76:87	arg1	Macrophages					89:99	THP-1 Cell-Derived Macrophages	70:99	THP-1 Cell-Derived Macrophages	70:99	A Biodegradable Mg-Based Alloy Inhibited the Inflammatory Response of THP-1 Cell-Derived Macrophages Through the TRPM7-PI3K-AKT1 Signaling Axis.
31849975	8	21	theme	species	1421:1427	arg1	expression					1435:1444	the LPS-induced reactive oxygen species (ROS) expression	1389:1444	the LPS-induced reactive oxygen species (ROS) expression	1389:1444	Additionally, the LPS-induced reactive oxygen species (ROS) expression was also decreased by extract and MgCl2.
31849975	4	22	theme	LPS	751:753	arg1	addition					739:746	the addition	735:746	the addition of LPS	735:753	THP-1 cell-derived macrophages were cultured on JDBM, Ti-6Al-4V alloy (Ti), 15% extract of JDBM, and 7.5 mM of MgCl2 for 1 h before the addition of LPS for an indicated time; the experiments included negative and positive controls.
31849975	7	23	theme	MYD88	1137:1141	arg1	expression					1092:1101	the expression	1088:1101	the expression of toll-like receptor (TLR)-4 and MYD88	1088:1141	Mechanically, extract and MgCl2 downregulated the expression of toll-like receptor (TLR)-4 and MYD88 compared with the positive control and inhibited LPS-induced nuclear factor-kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways by inactivation of the phosphorylation of IKK-α/β, IKβ-α, P65, P38, and JNK.
31849975	7	24	theme	mitogen-activated	1239:1255	arg1	kinase					1265:1270	mitogen-activated protein kinase	1239:1270	mitogen-activated protein kinase (MAPK) signaling pathways	1239:1296	Mechanically, extract and MgCl2 downregulated the expression of toll-like receptor (TLR)-4 and MYD88 compared with the positive control and inhibited LPS-induced nuclear factor-kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways by inactivation of the phosphorylation of IKK-α/β, IKβ-α, P65, P38, and JNK.
31849975	7	24	theme	mitogen-activated	1239:1255	arg1	MAPK					1273:1276	MAPK	1273:1276	MAPK	1273:1276	Mechanically, extract and MgCl2 downregulated the expression of toll-like receptor (TLR)-4 and MYD88 compared with the positive control and inhibited LPS-induced nuclear factor-kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways by inactivation of the phosphorylation of IKK-α/β, IKβ-α, P65, P38, and JNK.
31849975	0	25	theme	TRPM7-PI3K-AKT1	113:127	arg1	Axis					139:142	the TRPM7-PI3K-AKT1 Signaling Axis	109:142	the TRPM7-PI3K-AKT1 Signaling Axis	109:142	A Biodegradable Mg-Based Alloy Inhibited the Inflammatory Response of THP-1 Cell-Derived Macrophages Through the TRPM7-PI3K-AKT1 Signaling Axis.
31849975	1	26	theme	anti-inflammatory	313:329	arg1	properties					331:340	their anti-inflammatory properties	307:340	their anti-inflammatory properties	307:340	Mg-based alloys might be ideal biomaterials in clinical applications owing to favorable mechanical properties, biodegradability, biocompatibility, and especially their anti-inflammatory properties.
31849975	5	27	theme	factor	921:926	arg1	expression					957:966	LPS-induced tumor necrosis factor (TNF) and interleukin (IL)-6 expression	894:966	LPS-induced tumor necrosis factor (TNF) and interleukin (IL)-6 expression	894:966	Our results showed JDBM, extract, and MgCl2 could decrease LPS-induced tumor necrosis factor (TNF) and interleukin (IL)-6 expression.
31849975	7	28	theme	kinase	1265:1270	arg1	pathways					1289:1296	mitogen-activated protein kinase (MAPK) signaling pathways	1239:1296	mitogen-activated protein kinase (MAPK) signaling pathways	1239:1296	Mechanically, extract and MgCl2 downregulated the expression of toll-like receptor (TLR)-4 and MYD88 compared with the positive control and inhibited LPS-induced nuclear factor-kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways by inactivation of the phosphorylation of IKK-α/β, IKβ-α, P65, P38, and JNK.
31849975	3	29	theme	Mg-2.1Nd-0.2Zn-0.5Zr	509:528	arg1	alloy					530:534	a Mg-2.1Nd-0.2Zn-0.5Zr alloy	507:534	a Mg-2.1Nd-0.2Zn-0.5Zr alloy (denoted as JDBM)	507:552	Here, we investigated the effects of a Mg-2.1Nd-0.2Zn-0.5Zr alloy (denoted as JDBM) on lipopolysaccharide (LPS)-induced macrophages.
31849975	0	30	theme	Mg-Based	16:23	arg1	Alloy					25:29	A Biodegradable Mg-Based Alloy	0:29	A Biodegradable Mg-Based Alloy	0:29	A Biodegradable Mg-Based Alloy Inhibited the Inflammatory Response of THP-1 Cell-Derived Macrophages Through the TRPM7-PI3K-AKT1 Signaling Axis.
31849975	6	31	theme	morphologic	992:1002	arg1	changes					1004:1010	no morphologic changes	989:1010	no morphologic changes	989:1010	However, there were no morphologic changes in macrophages on Ti or JDBM.
31849975	11	32	theme	Mg-based	2061:2068	arg1	applications					2076:2087	biodegradable Mg-based alloy applications	2047:2087	biodegradable Mg-based alloy applications	2047:2087	Together, our results demonstrated the degradation products of Mg-based alloy, especially magnesium, and resolved inflammation by activation of the TRPM7-PI3K-AKT1 signaling pathway, which may be a potential advantage or target to promote biodegradable Mg-based alloy applications.
31849975	4	33	theme	cell-derived	609:620	arg1	macrophages					622:632	THP-1 cell-derived macrophages	603:632	THP-1 cell-derived macrophages	603:632	THP-1 cell-derived macrophages were cultured on JDBM, Ti-6Al-4V alloy (Ti), 15% extract of JDBM, and 7.5 mM of MgCl2 for 1 h before the addition of LPS for an indicated time; the experiments included negative and positive controls.
31849975	0	34	theme	Inflammatory	45:56	arg1	Response					58:65	the Inflammatory Response	41:65	the Inflammatory Response of THP-1 Cell-Derived Macrophages	41:99	A Biodegradable Mg-Based Alloy Inhibited the Inflammatory Response of THP-1 Cell-Derived Macrophages Through the TRPM7-PI3K-AKT1 Signaling Axis.
31849975	9	35	theme	down	1576:1579	arg1	TRPM7					1581:1585	knocking down TRPM7	1567:1585	knocking down TRPM7 of macrophages	1567:1600	Interestingly, the expression of LPS-induced TNF and IL-6 could be recovered by knocking down TRPM7 of macrophages, in the presence of extract or MgCl2.
31849975	9	36	theme	TNF	1532:1534	arg1	expression					1506:1515	the expression	1502:1515	the expression of LPS-induced TNF and IL-6	1502:1543	Interestingly, the expression of LPS-induced TNF and IL-6 could be recovered by knocking down TRPM7 of macrophages, in the presence of extract or MgCl2.
31849975	7	37	theme	factor-kappa	1212:1223	arg1	NF-κB					1228:1232	NF-κB	1228:1232	NF-κB	1228:1232	Mechanically, extract and MgCl2 downregulated the expression of toll-like receptor (TLR)-4 and MYD88 compared with the positive control and inhibited LPS-induced nuclear factor-kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways by inactivation of the phosphorylation of IKK-α/β, IKβ-α, P65, P38, and JNK.
31849975	7	37	theme	factor-kappa	1212:1223	arg1	B					1225:1225	LPS-induced nuclear factor-kappa B	1192:1225	LPS-induced nuclear factor-kappa B (NF-κB)	1192:1233	Mechanically, extract and MgCl2 downregulated the expression of toll-like receptor (TLR)-4 and MYD88 compared with the positive control and inhibited LPS-induced nuclear factor-kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways by inactivation of the phosphorylation of IKK-α/β, IKβ-α, P65, P38, and JNK.
31849975	2	38	theme	Mg-based	429:436	arg1	alloys					438:443	Mg-based alloys	429:443	Mg-based alloys	429:443	However, the precise signaling mechanism underlying the inhibition of inflammation by Mg-based alloys has not been elucidated.
31849975	9	39	theme	IL-6	1540:1543	arg1	expression					1506:1515	the expression	1502:1515	the expression of LPS-induced TNF and IL-6	1502:1543	Interestingly, the expression of LPS-induced TNF and IL-6 could be recovered by knocking down TRPM7 of macrophages, in the presence of extract or MgCl2.
31849975	7	40	theme	LPS-induced	1192:1202	arg1	NF-κB					1228:1232	NF-κB	1228:1232	NF-κB	1228:1232	Mechanically, extract and MgCl2 downregulated the expression of toll-like receptor (TLR)-4 and MYD88 compared with the positive control and inhibited LPS-induced nuclear factor-kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways by inactivation of the phosphorylation of IKK-α/β, IKβ-α, P65, P38, and JNK.
31849975	7	40	theme	LPS-induced	1192:1202	arg1	B					1225:1225	LPS-induced nuclear factor-kappa B	1192:1225	LPS-induced nuclear factor-kappa B (NF-κB)	1192:1233	Mechanically, extract and MgCl2 downregulated the expression of toll-like receptor (TLR)-4 and MYD88 compared with the positive control and inhibited LPS-induced nuclear factor-kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways by inactivation of the phosphorylation of IKK-α/β, IKβ-α, P65, P38, and JNK.
31849975	3	41	from	effects	496:502	arg1	macrophages					590:600	lipopolysaccharide (LPS)-induced macrophages	557:600	lipopolysaccharide (LPS)-induced macrophages	557:600	Here, we investigated the effects of a Mg-2.1Nd-0.2Zn-0.5Zr alloy (denoted as JDBM) on lipopolysaccharide (LPS)-induced macrophages.
31849975	2	42	theme	inflammation	413:424	arg1	inhibition					399:408	the inhibition	395:408	the inhibition of inflammation by Mg-based alloys	395:443	However, the precise signaling mechanism underlying the inhibition of inflammation by Mg-based alloys has not been elucidated.
31849975	3	43	theme	-induced	581:588	arg1	macrophages					590:600	lipopolysaccharide (LPS)-induced macrophages	557:600	lipopolysaccharide (LPS)-induced macrophages	557:600	Here, we investigated the effects of a Mg-2.1Nd-0.2Zn-0.5Zr alloy (denoted as JDBM) on lipopolysaccharide (LPS)-induced macrophages.
31849975	11	44	theme	magnesium	1898:1906	arg1	products					1859:1866	the degradation products	1843:1866	the degradation products of Mg-based alloy, especially magnesium, and resolved inflammation	1843:1933	Together, our results demonstrated the degradation products of Mg-based alloy, especially magnesium, and resolved inflammation by activation of the TRPM7-PI3K-AKT1 signaling pathway, which may be a potential advantage or target to promote biodegradable Mg-based alloy applications.
31849975	7	45	theme	JNK	1370:1372	arg1	phosphorylation					1321:1335	the phosphorylation	1317:1335	the phosphorylation of IKK-α/β, IKβ-α, P65, P38, and JNK	1317:1372	Mechanically, extract and MgCl2 downregulated the expression of toll-like receptor (TLR)-4 and MYD88 compared with the positive control and inhibited LPS-induced nuclear factor-kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways by inactivation of the phosphorylation of IKK-α/β, IKβ-α, P65, P38, and JNK.
31849975	11	46	theme	pathway	1982:1988	arg1	activation					1938:1947	activation	1938:1947	activation of the TRPM7-PI3K-AKT1 signaling pathway, which may be a potential advantage or target to promote biodegradable Mg-based alloy applications	1938:2087	Together, our results demonstrated the degradation products of Mg-based alloy, especially magnesium, and resolved inflammation by activation of the TRPM7-PI3K-AKT1 signaling pathway, which may be a potential advantage or target to promote biodegradable Mg-based alloy applications.
31849975	7	47	theme	P38	1361:1363	arg1	phosphorylation					1321:1335	the phosphorylation	1317:1335	the phosphorylation of IKK-α/β, IKβ-α, P65, P38, and JNK	1317:1372	Mechanically, extract and MgCl2 downregulated the expression of toll-like receptor (TLR)-4 and MYD88 compared with the positive control and inhibited LPS-induced nuclear factor-kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways by inactivation of the phosphorylation of IKK-α/β, IKβ-α, P65, P38, and JNK.
31849975	4	48	theme	positive	816:823	arg1	controls					825:832	negative and positive controls	803:832	negative and positive controls	803:832	THP-1 cell-derived macrophages were cultured on JDBM, Ti-6Al-4V alloy (Ti), 15% extract of JDBM, and 7.5 mM of MgCl2 for 1 h before the addition of LPS for an indicated time; the experiments included negative and positive controls.
31849975	7	49	theme	P65	1356:1358	arg1	phosphorylation					1321:1335	the phosphorylation	1317:1335	the phosphorylation of IKK-α/β, IKβ-α, P65, P38, and JNK	1317:1372	Mechanically, extract and MgCl2 downregulated the expression of toll-like receptor (TLR)-4 and MYD88 compared with the positive control and inhibited LPS-induced nuclear factor-kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways by inactivation of the phosphorylation of IKK-α/β, IKβ-α, P65, P38, and JNK.
31849975	4	50	theme	%	681:681	arg1	extract					683:689	15% extract	679:689	15% extract of JDBM	679:697	THP-1 cell-derived macrophages were cultured on JDBM, Ti-6Al-4V alloy (Ti), 15% extract of JDBM, and 7.5 mM of MgCl2 for 1 h before the addition of LPS for an indicated time; the experiments included negative and positive controls.
31849975	10	51	theme	AKT	1672:1674	arg1	activities					1658:1667	the activities	1654:1667	the activities of AKT and AKT1	1654:1683	Mechanically, the activities of AKT and AKT1 were increased by extract or MgCl2 with LPS and were blocked by a PI3K inhibitor, whereas siRNA TRPM7 inhibited only AKT1.
31849975	7	52	theme	IKβ-α	1349:1353	arg1	phosphorylation					1321:1335	the phosphorylation	1317:1335	the phosphorylation of IKK-α/β, IKβ-α, P65, P38, and JNK	1317:1372	Mechanically, extract and MgCl2 downregulated the expression of toll-like receptor (TLR)-4 and MYD88 compared with the positive control and inhibited LPS-induced nuclear factor-kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways by inactivation of the phosphorylation of IKK-α/β, IKβ-α, P65, P38, and JNK.
31849975	4	53	theme	negative	803:810	arg1	controls					825:832	negative and positive controls	803:832	negative and positive controls	803:832	THP-1 cell-derived macrophages were cultured on JDBM, Ti-6Al-4V alloy (Ti), 15% extract of JDBM, and 7.5 mM of MgCl2 for 1 h before the addition of LPS for an indicated time; the experiments included negative and positive controls.
31849975	7	54	theme	IKK-α/β	1340:1346	arg1	phosphorylation					1321:1335	the phosphorylation	1317:1335	the phosphorylation of IKK-α/β, IKβ-α, P65, P38, and JNK	1317:1372	Mechanically, extract and MgCl2 downregulated the expression of toll-like receptor (TLR)-4 and MYD88 compared with the positive control and inhibited LPS-induced nuclear factor-kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways by inactivation of the phosphorylation of IKK-α/β, IKβ-α, P65, P38, and JNK.
31849975	1	55	theme	mechanical	233:242	arg1	properties					244:253	favorable mechanical properties	223:253	favorable mechanical properties	223:253	Mg-based alloys might be ideal biomaterials in clinical applications owing to favorable mechanical properties, biodegradability, biocompatibility, and especially their anti-inflammatory properties.
31849975	11	56	theme	resolved	1913:1920	arg1	inflammation					1922:1933	resolved inflammation	1913:1933	resolved inflammation	1913:1933	Together, our results demonstrated the degradation products of Mg-based alloy, especially magnesium, and resolved inflammation by activation of the TRPM7-PI3K-AKT1 signaling pathway, which may be a potential advantage or target to promote biodegradable Mg-based alloy applications.
31849975	7	57	theme	phosphorylation	1321:1335	arg1	inactivation					1301:1312	inactivation	1301:1312	inactivation of the phosphorylation of IKK-α/β, IKβ-α, P65, P38, and JNK	1301:1372	Mechanically, extract and MgCl2 downregulated the expression of toll-like receptor (TLR)-4 and MYD88 compared with the positive control and inhibited LPS-induced nuclear factor-kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways by inactivation of the phosphorylation of IKK-α/β, IKβ-α, P65, P38, and JNK.
31849975	10	58	theme	AKT1	1680:1683	arg1	activities					1658:1667	the activities	1654:1667	the activities of AKT and AKT1	1654:1683	Mechanically, the activities of AKT and AKT1 were increased by extract or MgCl2 with LPS and were blocked by a PI3K inhibitor, whereas siRNA TRPM7 inhibited only AKT1.
31849975	11	59	theme	TRPM7-PI3K-AKT1	1956:1970	arg1	advantage					2016:2024	a potential advantage	2004:2024	a potential advantage	2004:2024	Together, our results demonstrated the degradation products of Mg-based alloy, especially magnesium, and resolved inflammation by activation of the TRPM7-PI3K-AKT1 signaling pathway, which may be a potential advantage or target to promote biodegradable Mg-based alloy applications.
31849975	11	59	theme	TRPM7-PI3K-AKT1	1956:1970	arg1	pathway					1982:1988	the TRPM7-PI3K-AKT1 signaling pathway	1952:1988	the TRPM7-PI3K-AKT1 signaling pathway	1952:1988	Together, our results demonstrated the degradation products of Mg-based alloy, especially magnesium, and resolved inflammation by activation of the TRPM7-PI3K-AKT1 signaling pathway, which may be a potential advantage or target to promote biodegradable Mg-based alloy applications.
31849975	5	60	theme	IL	951:952	arg1	expression					957:966	LPS-induced tumor necrosis factor (TNF) and interleukin (IL)-6 expression	894:966	LPS-induced tumor necrosis factor (TNF) and interleukin (IL)-6 expression	894:966	Our results showed JDBM, extract, and MgCl2 could decrease LPS-induced tumor necrosis factor (TNF) and interleukin (IL)-6 expression.
31849975	1	61	theme	Mg-based	145:152	arg1	alloys					154:159	Mg-based alloys	145:159	Mg-based alloys	145:159	Mg-based alloys might be ideal biomaterials in clinical applications owing to favorable mechanical properties, biodegradability, biocompatibility, and especially their anti-inflammatory properties.
31849975	8	62	theme	LPS-induced	1393:1403	arg1	expression					1435:1444	the LPS-induced reactive oxygen species (ROS) expression	1389:1444	the LPS-induced reactive oxygen species (ROS) expression	1389:1444	Additionally, the LPS-induced reactive oxygen species (ROS) expression was also decreased by extract and MgCl2.
31849975	0	63	theme	THP-1	70:74	arg1	Macrophages					89:99	THP-1 Cell-Derived Macrophages	70:99	THP-1 Cell-Derived Macrophages	70:99	A Biodegradable Mg-Based Alloy Inhibited the Inflammatory Response of THP-1 Cell-Derived Macrophages Through the TRPM7-PI3K-AKT1 Signaling Axis.
31849975	11	64	theme	inflammation	1922:1933	arg1	products					1859:1866	the degradation products	1843:1866	the degradation products of Mg-based alloy, especially magnesium, and resolved inflammation	1843:1933	Together, our results demonstrated the degradation products of Mg-based alloy, especially magnesium, and resolved inflammation by activation of the TRPM7-PI3K-AKT1 signaling pathway, which may be a potential advantage or target to promote biodegradable Mg-based alloy applications.
31849975	8	65	theme	oxygen	1414:1419	arg1	species					1421:1427	reactive oxygen species	1405:1427	the LPS-induced reactive oxygen species (ROS) expression	1389:1444	Additionally, the LPS-induced reactive oxygen species (ROS) expression was also decreased by extract and MgCl2.
31849975	8	65	theme	oxygen	1414:1419	arg1	ROS					1430:1432	ROS	1430:1432	ROS	1430:1432	Additionally, the LPS-induced reactive oxygen species (ROS) expression was also decreased by extract and MgCl2.
31849975	7	66	theme	signaling	1279:1287	arg1	pathways					1289:1296	mitogen-activated protein kinase (MAPK) signaling pathways	1239:1296	mitogen-activated protein kinase (MAPK) signaling pathways	1239:1296	Mechanically, extract and MgCl2 downregulated the expression of toll-like receptor (TLR)-4 and MYD88 compared with the positive control and inhibited LPS-induced nuclear factor-kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways by inactivation of the phosphorylation of IKK-α/β, IKβ-α, P65, P38, and JNK.
31849975	0	67	theme	Macrophages	89:99	arg1	Response					58:65	the Inflammatory Response	41:65	the Inflammatory Response of THP-1 Cell-Derived Macrophages	41:99	A Biodegradable Mg-Based Alloy Inhibited the Inflammatory Response of THP-1 Cell-Derived Macrophages Through the TRPM7-PI3K-AKT1 Signaling Axis.
31849975	1	68	theme	ideal	170:174	arg1	biomaterials					176:187	ideal biomaterials	170:187	ideal biomaterials	170:187	Mg-based alloys might be ideal biomaterials in clinical applications owing to favorable mechanical properties, biodegradability, biocompatibility, and especially their anti-inflammatory properties.
31849975	1	69	from	biomaterials	176:187	arg1	applications					201:212	clinical applications	192:212	clinical applications owing to favorable mechanical properties, biodegradability, biocompatibility, and especially their anti-inflammatory properties	192:340	Mg-based alloys might be ideal biomaterials in clinical applications owing to favorable mechanical properties, biodegradability, biocompatibility, and especially their anti-inflammatory properties.
31849975	0	70	theme	Signaling	129:137	arg1	Axis					139:142	the TRPM7-PI3K-AKT1 Signaling Axis	109:142	the TRPM7-PI3K-AKT1 Signaling Axis	109:142	A Biodegradable Mg-Based Alloy Inhibited the Inflammatory Response of THP-1 Cell-Derived Macrophages Through the TRPM7-PI3K-AKT1 Signaling Axis.
31849975	5	71	theme	interleukin	938:948	arg1	IL					951:952	interleukin (IL)-6	938:955	interleukin (IL)-6	938:955	Our results showed JDBM, extract, and MgCl2 could decrease LPS-induced tumor necrosis factor (TNF) and interleukin (IL)-6 expression.
31849975	11	72	theme	potential	2006:2014	arg1	advantage					2016:2024	a potential advantage	2004:2024	a potential advantage	2004:2024	Together, our results demonstrated the degradation products of Mg-based alloy, especially magnesium, and resolved inflammation by activation of the TRPM7-PI3K-AKT1 signaling pathway, which may be a potential advantage or target to promote biodegradable Mg-based alloy applications.
31849975	11	72	theme	potential	2006:2014	arg1	pathway					1982:1988	the TRPM7-PI3K-AKT1 signaling pathway	1952:1988	the TRPM7-PI3K-AKT1 signaling pathway	1952:1988	Together, our results demonstrated the degradation products of Mg-based alloy, especially magnesium, and resolved inflammation by activation of the TRPM7-PI3K-AKT1 signaling pathway, which may be a potential advantage or target to promote biodegradable Mg-based alloy applications.
31849975	0	73	theme	Biodegradable	2:14	arg1	Alloy					25:29	A Biodegradable Mg-Based Alloy	0:29	A Biodegradable Mg-Based Alloy	0:29	A Biodegradable Mg-Based Alloy Inhibited the Inflammatory Response of THP-1 Cell-Derived Macrophages Through the TRPM7-PI3K-AKT1 Signaling Axis.
31849975	5	74	theme	necrosis	912:919	arg1	TNF					929:931	TNF	929:931	TNF	929:931	Our results showed JDBM, extract, and MgCl2 could decrease LPS-induced tumor necrosis factor (TNF) and interleukin (IL)-6 expression.
31849975	5	74	theme	necrosis	912:919	arg1	factor					921:926	tumor necrosis factor	906:926	tumor necrosis factor (TNF)	906:932	Our results showed JDBM, extract, and MgCl2 could decrease LPS-induced tumor necrosis factor (TNF) and interleukin (IL)-6 expression.
31849975	7	75	theme	protein	1257:1263	arg1	kinase					1265:1270	mitogen-activated protein kinase	1239:1270	mitogen-activated protein kinase (MAPK) signaling pathways	1239:1296	Mechanically, extract and MgCl2 downregulated the expression of toll-like receptor (TLR)-4 and MYD88 compared with the positive control and inhibited LPS-induced nuclear factor-kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways by inactivation of the phosphorylation of IKK-α/β, IKβ-α, P65, P38, and JNK.
31849975	7	75	theme	protein	1257:1263	arg1	MAPK					1273:1276	MAPK	1273:1276	MAPK	1273:1276	Mechanically, extract and MgCl2 downregulated the expression of toll-like receptor (TLR)-4 and MYD88 compared with the positive control and inhibited LPS-induced nuclear factor-kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways by inactivation of the phosphorylation of IKK-α/β, IKβ-α, P65, P38, and JNK.
31849975	4	76	theme	THP-1	603:607	arg1	macrophages					622:632	THP-1 cell-derived macrophages	603:632	THP-1 cell-derived macrophages	603:632	THP-1 cell-derived macrophages were cultured on JDBM, Ti-6Al-4V alloy (Ti), 15% extract of JDBM, and 7.5 mM of MgCl2 for 1 h before the addition of LPS for an indicated time; the experiments included negative and positive controls.
31849975	7	77	theme	toll-like	1106:1114	arg1	receptor					1116:1123	toll-like receptor (TLR)-4	1106:1131	toll-like receptor (TLR)-4	1106:1131	Mechanically, extract and MgCl2 downregulated the expression of toll-like receptor (TLR)-4 and MYD88 compared with the positive control and inhibited LPS-induced nuclear factor-kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways by inactivation of the phosphorylation of IKK-α/β, IKβ-α, P65, P38, and JNK.
31849975	7	77	theme	toll-like	1106:1114	arg1	TLR					1126:1128	TLR	1126:1128	TLR	1126:1128	Mechanically, extract and MgCl2 downregulated the expression of toll-like receptor (TLR)-4 and MYD88 compared with the positive control and inhibited LPS-induced nuclear factor-kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways by inactivation of the phosphorylation of IKK-α/β, IKβ-α, P65, P38, and JNK.
31849975	3	78	theme	alloy	530:534	arg1	effects					496:502	the effects	492:502	the effects of a Mg-2.1Nd-0.2Zn-0.5Zr alloy (denoted as JDBM) on lipopolysaccharide (LPS)-induced macrophages	492:600	Here, we investigated the effects of a Mg-2.1Nd-0.2Zn-0.5Zr alloy (denoted as JDBM) on lipopolysaccharide (LPS)-induced macrophages.
31849975	11	79	theme	alloy	2070:2074	arg1	applications					2076:2087	biodegradable Mg-based alloy applications	2047:2087	biodegradable Mg-based alloy applications	2047:2087	Together, our results demonstrated the degradation products of Mg-based alloy, especially magnesium, and resolved inflammation by activation of the TRPM7-PI3K-AKT1 signaling pathway, which may be a potential advantage or target to promote biodegradable Mg-based alloy applications.
31849975	11	80	theme	biodegradable	2047:2059	arg1	applications					2076:2087	biodegradable Mg-based alloy applications	2047:2087	biodegradable Mg-based alloy applications	2047:2087	Together, our results demonstrated the degradation products of Mg-based alloy, especially magnesium, and resolved inflammation by activation of the TRPM7-PI3K-AKT1 signaling pathway, which may be a potential advantage or target to promote biodegradable Mg-based alloy applications.
31849975	10	81	theme	PI3K	1751:1754	arg1	inhibitor					1756:1764	a PI3K inhibitor	1749:1764	a PI3K inhibitor	1749:1764	Mechanically, the activities of AKT and AKT1 were increased by extract or MgCl2 with LPS and were blocked by a PI3K inhibitor, whereas siRNA TRPM7 inhibited only AKT1.
31849975	5	82	dep	showed	847:852	arg1	decrease					885:892	decrease	885:892	showed JDBM, extract, and MgCl2 could decrease LPS-induced tumor necrosis factor (TNF) and interleukin (IL)-6 expression	847:966	Our results showed JDBM, extract, and MgCl2 could decrease LPS-induced tumor necrosis factor (TNF) and interleukin (IL)-6 expression.
31849975	9	83	theme	LPS-induced	1520:1530	arg1	TNF					1532:1534	LPS-induced TNF	1520:1534	LPS-induced TNF	1520:1534	Interestingly, the expression of LPS-induced TNF and IL-6 could be recovered by knocking down TRPM7 of macrophages, in the presence of extract or MgCl2.
31849975	7	84	theme	nuclear	1204:1210	arg1	NF-κB					1228:1232	NF-κB	1228:1232	NF-κB	1228:1232	Mechanically, extract and MgCl2 downregulated the expression of toll-like receptor (TLR)-4 and MYD88 compared with the positive control and inhibited LPS-induced nuclear factor-kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways by inactivation of the phosphorylation of IKK-α/β, IKβ-α, P65, P38, and JNK.
31849975	7	84	theme	nuclear	1204:1210	arg1	B					1225:1225	LPS-induced nuclear factor-kappa B	1192:1225	LPS-induced nuclear factor-kappa B (NF-κB)	1192:1233	Mechanically, extract and MgCl2 downregulated the expression of toll-like receptor (TLR)-4 and MYD88 compared with the positive control and inhibited LPS-induced nuclear factor-kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways by inactivation of the phosphorylation of IKK-α/β, IKβ-α, P65, P38, and JNK.
31849975	4	85	theme	Ti-6Al-4V	657:665	arg1	Ti					674:675	Ti	674:675	Ti	674:675	THP-1 cell-derived macrophages were cultured on JDBM, Ti-6Al-4V alloy (Ti), 15% extract of JDBM, and 7.5 mM of MgCl2 for 1 h before the addition of LPS for an indicated time; the experiments included negative and positive controls.
31849975	4	85	theme	Ti-6Al-4V	657:665	arg1	alloy					667:671	Ti-6Al-4V alloy	657:671	Ti-6Al-4V alloy (Ti)	657:676	THP-1 cell-derived macrophages were cultured on JDBM, Ti-6Al-4V alloy (Ti), 15% extract of JDBM, and 7.5 mM of MgCl2 for 1 h before the addition of LPS for an indicated time; the experiments included negative and positive controls.
31849975	4	86	theme	indicated	762:770	arg1	time					772:775	an indicated time	759:775	an indicated time	759:775	THP-1 cell-derived macrophages were cultured on JDBM, Ti-6Al-4V alloy (Ti), 15% extract of JDBM, and 7.5 mM of MgCl2 for 1 h before the addition of LPS for an indicated time; the experiments included negative and positive controls.
31849975	11	87	theme	degradation	1847:1857	arg1	products					1859:1866	the degradation products	1843:1866	the degradation products of Mg-based alloy, especially magnesium, and resolved inflammation	1843:1933	Together, our results demonstrated the degradation products of Mg-based alloy, especially magnesium, and resolved inflammation by activation of the TRPM7-PI3K-AKT1 signaling pathway, which may be a potential advantage or target to promote biodegradable Mg-based alloy applications.
31849975	10	88	theme	siRNA	1775:1779	arg1	TRPM7					1781:1785	siRNA TRPM7	1775:1785	siRNA TRPM7	1775:1785	Mechanically, the activities of AKT and AKT1 were increased by extract or MgCl2 with LPS and were blocked by a PI3K inhibitor, whereas siRNA TRPM7 inhibited only AKT1.
30026325	7	0	theme	OST	1098:1100	arg1	inhibition					1102:1111	OST inhibition	1098:1111	OST inhibition	1098:1111	OST inhibition invariably disrupted EGFR N-linked glycosylation and reduced activation of receptors either with or without the T790M TKI resistance mutation.
30026325	6	1	from	cells	1041:1045	arg1	lethal					1031:1036	lethal	1031:1036	lethal	1031:1036	Addition of NGI-1 to EGFR TKI treatment was synthetic lethal in cells resistant to gefitinib, erlotinib, or osimertinib.
30026325	10	2	theme	EGFR	1931:1934	arg1	TKIs					1936:1939	EGFR TKIs	1931:1939	EGFR TKIs	1931:1939	This therapeutic strategy breaks from kinase-targeted approaches and validates N-linked glycosylation as an effective target in tumors driven by glycoprotein signaling.Significance:EGFR-mutant NSCLC is incurable despite the marked sensitivity of these tumors to EGFR TKIs.
30026325	9	3	dep	in	1527:1528	arg1	vivo					1530:1533	vivo	1530:1533	vivo	1530:1533	Translation of this approach to preclinical models was accomplished through synthesis and delivery of NGI-1 nanoparticles, confirmation of in vivo activity through molecular imaging, and demonstration of significant tumor growth delay in TKI-resistant HCC827 and H1975 xenografts.
30026325	5	4	theme	kinase	815:820	arg1	TKI					833:835	TKI	833:835	TKI	833:835	In EGFR-mutant non-small cell lung cancer with EGFR tyrosine kinase inhibitor (TKI) resistance (PC9-GR, HCC827-GR, and H1975-OR), OST inhibition maintained its ability to induce cell-cycle arrest and a proliferative block.
30026325	5	4	theme	kinase	815:820	arg1	inhibitor					822:830	EGFR tyrosine kinase inhibitor	801:830	EGFR tyrosine kinase inhibitor (TKI) resistance (PC9-GR, HCC827-GR, and H1975-OR)	801:881	In EGFR-mutant non-small cell lung cancer with EGFR tyrosine kinase inhibitor (TKI) resistance (PC9-GR, HCC827-GR, and H1975-OR), OST inhibition maintained its ability to induce cell-cycle arrest and a proliferative block.
30026325	10	5	from	target	1787:1792	arg1	tumors					1797:1802	tumors	1797:1802	tumors driven by glycoprotein signaling.Significance	1797:1848	This therapeutic strategy breaks from kinase-targeted approaches and validates N-linked glycosylation as an effective target in tumors driven by glycoprotein signaling.Significance:EGFR-mutant NSCLC is incurable despite the marked sensitivity of these tumors to EGFR TKIs.
30026325	4	6	theme	cell	678:681	arg1	lines					683:687	just 11 of 94 (12%) cell lines	658:687	just 11 of 94 (12%) cell lines	658:687	This screen revealed NGI-1 sensitivity in just 11 of 94 (12%) cell lines, with a significant correlation between OST and EGFR inhibitors.
30026325	6	7	from	lethal	1031:1036	arg1	cells					1041:1045	cells	1041:1045	cells resistant to gefitinib, erlotinib, or osimertinib	1041:1095	Addition of NGI-1 to EGFR TKI treatment was synthetic lethal in cells resistant to gefitinib, erlotinib, or osimertinib.
30026325	3	8	theme	viability	605:613	arg1	loss					586:589	a selective loss	574:589	a selective loss of tumor cell viability	574:613	Here, we perform sensitivity screening of 94 lung cancer cell lines using NGI-1, a small-molecule inhibitor of the oligosaccharyltransferase (OST) that partially disrupts N-linked glycosylation, and demonstrate a selective loss of tumor cell viability.
30026325	1	9	theme	transmembrane	216:228	arg1	proteins					230:237	complex transmembrane proteins	208:237	complex transmembrane proteins	208:237	Asparagine (N)-linked glycosylation is a posttranslational modification essential for the function of complex transmembrane proteins.
30026325	3	10	link	N-linked	534:541	arg1	glycosylation					543:555	N-linked glycosylation	534:555	N-linked glycosylation	534:555	Here, we perform sensitivity screening of 94 lung cancer cell lines using NGI-1, a small-molecule inhibitor of the oligosaccharyltransferase (OST) that partially disrupts N-linked glycosylation, and demonstrate a selective loss of tumor cell viability.
30026325	11	11	theme	oncogenic	2048:2056	arg1	proteins					2068:2075	other oncogenic signaling proteins	2042:2075	other oncogenic signaling proteins	2042:2075	These findings identify N-linked glycosylation, a posttranslational modification common to EGFR and other oncogenic signaling proteins, as an effective therapeutic target that enhances tumor responses for EGFR-mutant NSCLC.
30026325	8	12	theme	OST	1256:1258	arg1	inhibition					1260:1269	OST inhibition	1256:1269	OST inhibition	1256:1269	OST inhibition also dissociated EGFR signaling from other coexpressed receptors like MET via altered receptor compartmentalization.
30026325	13	13	theme	©2018	2196:2200	arg1	AACR					2202:2205	©2018 AACR	2196:2205	©2018 AACR.	2196:2206	©2018 AACR.
30026325	3	14	theme	tumor	594:598	arg1	viability					605:613	tumor cell viability	594:613	tumor cell viability	594:613	Here, we perform sensitivity screening of 94 lung cancer cell lines using NGI-1, a small-molecule inhibitor of the oligosaccharyltransferase (OST) that partially disrupts N-linked glycosylation, and demonstrate a selective loss of tumor cell viability.
30026325	9	15	theme	nanoparticles	1496:1508	arg1	delivery					1478:1485	delivery	1478:1485	delivery	1478:1485	Translation of this approach to preclinical models was accomplished through synthesis and delivery of NGI-1 nanoparticles, confirmation of in vivo activity through molecular imaging, and demonstration of significant tumor growth delay in TKI-resistant HCC827 and H1975 xenografts.
30026325	9	15	theme	nanoparticles	1496:1508	arg1	synthesis					1464:1472	synthesis	1464:1472	synthesis	1464:1472	Translation of this approach to preclinical models was accomplished through synthesis and delivery of NGI-1 nanoparticles, confirmation of in vivo activity through molecular imaging, and demonstration of significant tumor growth delay in TKI-resistant HCC827 and H1975 xenografts.
30026325	11	16	link	N-linked	1966:1973	arg1	modification					2010:2021	a posttranslational modification	1990:2021	a posttranslational modification common to EGFR and other oncogenic signaling proteins	1990:2075	These findings identify N-linked glycosylation, a posttranslational modification common to EGFR and other oncogenic signaling proteins, as an effective therapeutic target that enhances tumor responses for EGFR-mutant NSCLC.
30026325	11	16	link	N-linked	1966:1973	arg1	target					2106:2111	an effective therapeutic target	2081:2111	an effective therapeutic target that enhances tumor responses for EGFR-mutant NSCLC	2081:2163	These findings identify N-linked glycosylation, a posttranslational modification common to EGFR and other oncogenic signaling proteins, as an effective therapeutic target that enhances tumor responses for EGFR-mutant NSCLC.
30026325	11	16	link	N-linked	1966:1973	arg1	glycosylation					1975:1987	N-linked glycosylation	1966:1987	N-linked glycosylation	1966:1987	These findings identify N-linked glycosylation, a posttranslational modification common to EGFR and other oncogenic signaling proteins, as an effective therapeutic target that enhances tumor responses for EGFR-mutant NSCLC.
30026325	10	17	theme	strategy	1686:1693	arg1	breaks					1695:1700	This therapeutic strategy breaks	1669:1700	This therapeutic strategy breaks from kinase-targeted approaches	1669:1732	This therapeutic strategy breaks from kinase-targeted approaches and validates N-linked glycosylation as an effective target in tumors driven by glycoprotein signaling.Significance:EGFR-mutant NSCLC is incurable despite the marked sensitivity of these tumors to EGFR TKIs.
30026325	5	18	theme	inhibitor	822:830	arg1	resistance					838:847	EGFR tyrosine kinase inhibitor (TKI) resistance	801:847	EGFR tyrosine kinase inhibitor (TKI) resistance (PC9-GR, HCC827-GR, and H1975-OR)	801:881	In EGFR-mutant non-small cell lung cancer with EGFR tyrosine kinase inhibitor (TKI) resistance (PC9-GR, HCC827-GR, and H1975-OR), OST inhibition maintained its ability to induce cell-cycle arrest and a proliferative block.
30026325	9	19	theme	confirmation	1511:1522	arg1	delivery					1478:1485	delivery	1478:1485	delivery	1478:1485	Translation of this approach to preclinical models was accomplished through synthesis and delivery of NGI-1 nanoparticles, confirmation of in vivo activity through molecular imaging, and demonstration of significant tumor growth delay in TKI-resistant HCC827 and H1975 xenografts.
30026325	9	19	theme	confirmation	1511:1522	arg1	synthesis					1464:1472	synthesis	1464:1472	synthesis	1464:1472	Translation of this approach to preclinical models was accomplished through synthesis and delivery of NGI-1 nanoparticles, confirmation of in vivo activity through molecular imaging, and demonstration of significant tumor growth delay in TKI-resistant HCC827 and H1975 xenografts.
30026325	8	20	theme	EGFR	1288:1291	arg1	signaling					1293:1301	EGFR signaling	1288:1301	EGFR signaling	1288:1301	OST inhibition also dissociated EGFR signaling from other coexpressed receptors like MET via altered receptor compartmentalization.
30026325	2	21	theme	targeting	249:257	arg1	glycosylation					259:271	targeting glycosylation	249:271	targeting glycosylation for cancer therapy	249:290	However, targeting glycosylation for cancer therapy has not been feasible due to generalized effects on all glycoproteins.
30026325	9	22	theme	in	1527:1528	arg1	activity					1535:1542	in vivo activity	1527:1542	in vivo activity through molecular imaging	1527:1568	Translation of this approach to preclinical models was accomplished through synthesis and delivery of NGI-1 nanoparticles, confirmation of in vivo activity through molecular imaging, and demonstration of significant tumor growth delay in TKI-resistant HCC827 and H1975 xenografts.
30026325	9	23	from	delay	1617:1621	arg1	xenografts					1657:1666	TKI-resistant HCC827 and H1975 xenografts	1626:1666	TKI-resistant HCC827 and H1975 xenografts	1626:1666	Translation of this approach to preclinical models was accomplished through synthesis and delivery of NGI-1 nanoparticles, confirmation of in vivo activity through molecular imaging, and demonstration of significant tumor growth delay in TKI-resistant HCC827 and H1975 xenografts.
30026325	3	24	theme	selective	576:584	arg1	loss					586:589	a selective loss	574:589	a selective loss of tumor cell viability	574:613	Here, we perform sensitivity screening of 94 lung cancer cell lines using NGI-1, a small-molecule inhibitor of the oligosaccharyltransferase (OST) that partially disrupts N-linked glycosylation, and demonstrate a selective loss of tumor cell viability.
30026325	9	25	theme	activity	1535:1542	arg1	nanoparticles					1496:1508	NGI-1 nanoparticles	1490:1508	NGI-1 nanoparticles	1490:1508	Translation of this approach to preclinical models was accomplished through synthesis and delivery of NGI-1 nanoparticles, confirmation of in vivo activity through molecular imaging, and demonstration of significant tumor growth delay in TKI-resistant HCC827 and H1975 xenografts.
30026325	9	25	theme	activity	1535:1542	arg1	demonstration					1575:1587	demonstration	1575:1587	demonstration of significant tumor growth delay in TKI-resistant HCC827 and H1975 xenografts	1575:1666	Translation of this approach to preclinical models was accomplished through synthesis and delivery of NGI-1 nanoparticles, confirmation of in vivo activity through molecular imaging, and demonstration of significant tumor growth delay in TKI-resistant HCC827 and H1975 xenografts.
30026325	9	25	theme	activity	1535:1542	arg1	confirmation					1511:1522	confirmation	1511:1522	confirmation of in vivo activity through molecular imaging	1511:1568	Translation of this approach to preclinical models was accomplished through synthesis and delivery of NGI-1 nanoparticles, confirmation of in vivo activity through molecular imaging, and demonstration of significant tumor growth delay in TKI-resistant HCC827 and H1975 xenografts.
30026325	6	26	theme	EGFR	998:1001	arg1	treatment					1007:1015	EGFR TKI treatment	998:1015	EGFR TKI treatment	998:1015	Addition of NGI-1 to EGFR TKI treatment was synthetic lethal in cells resistant to gefitinib, erlotinib, or osimertinib.
30026325	8	27	theme	coexpressed	1314:1324	arg1	receptors					1326:1334	other coexpressed receptors	1308:1334	other coexpressed receptors like MET	1308:1343	OST inhibition also dissociated EGFR signaling from other coexpressed receptors like MET via altered receptor compartmentalization.
30026325	0	28	theme	Kinase	87:92	arg1	Inhibitors					94:103	EGFR Tyrosine Kinase Inhibitors	73:103	EGFR Tyrosine Kinase Inhibitors	73:103	Oligosaccharyltransferase Inhibition Overcomes Therapeutic Resistance to EGFR Tyrosine Kinase Inhibitors.
30026325	9	29	theme	molecular	1552:1560	arg1	imaging					1562:1568	molecular imaging	1552:1568	molecular imaging	1552:1568	Translation of this approach to preclinical models was accomplished through synthesis and delivery of NGI-1 nanoparticles, confirmation of in vivo activity through molecular imaging, and demonstration of significant tumor growth delay in TKI-resistant HCC827 and H1975 xenografts.
30026325	6	30	theme	NGI-1	989:993	arg1	Addition					977:984	Addition	977:984	Addition of NGI-1 to EGFR TKI treatment	977:1015	Addition of NGI-1 to EGFR TKI treatment was synthetic lethal in cells resistant to gefitinib, erlotinib, or osimertinib.
30026325	5	31	theme	cell	779:782	arg1	cancer					789:794	EGFR-mutant non-small cell lung cancer	757:794	EGFR-mutant non-small cell lung cancer with EGFR tyrosine kinase inhibitor (TKI) resistance (PC9-GR, HCC827-GR, and H1975-OR)	757:881	In EGFR-mutant non-small cell lung cancer with EGFR tyrosine kinase inhibitor (TKI) resistance (PC9-GR, HCC827-GR, and H1975-OR), OST inhibition maintained its ability to induce cell-cycle arrest and a proliferative block.
30026325	10	32	theme	tumors	1921:1926	arg1	sensitivity					1900:1910	the marked sensitivity	1889:1910	the marked sensitivity of these tumors to EGFR TKIs	1889:1939	This therapeutic strategy breaks from kinase-targeted approaches and validates N-linked glycosylation as an effective target in tumors driven by glycoprotein signaling.Significance:EGFR-mutant NSCLC is incurable despite the marked sensitivity of these tumors to EGFR TKIs.
30026325	5	33	theme	cell-cycle	932:941	arg1	arrest					943:948	cell-cycle arrest	932:948	cell-cycle arrest	932:948	In EGFR-mutant non-small cell lung cancer with EGFR tyrosine kinase inhibitor (TKI) resistance (PC9-GR, HCC827-GR, and H1975-OR), OST inhibition maintained its ability to induce cell-cycle arrest and a proliferative block.
30026325	10	34	theme	marked	1893:1898	arg1	sensitivity					1900:1910	the marked sensitivity	1889:1910	the marked sensitivity of these tumors to EGFR TKIs	1889:1939	This therapeutic strategy breaks from kinase-targeted approaches and validates N-linked glycosylation as an effective target in tumors driven by glycoprotein signaling.Significance:EGFR-mutant NSCLC is incurable despite the marked sensitivity of these tumors to EGFR TKIs.
30026325	9	35	theme	demonstration	1575:1587	arg1	delivery					1478:1485	delivery	1478:1485	delivery	1478:1485	Translation of this approach to preclinical models was accomplished through synthesis and delivery of NGI-1 nanoparticles, confirmation of in vivo activity through molecular imaging, and demonstration of significant tumor growth delay in TKI-resistant HCC827 and H1975 xenografts.
30026325	9	35	theme	demonstration	1575:1587	arg1	synthesis					1464:1472	synthesis	1464:1472	synthesis	1464:1472	Translation of this approach to preclinical models was accomplished through synthesis and delivery of NGI-1 nanoparticles, confirmation of in vivo activity through molecular imaging, and demonstration of significant tumor growth delay in TKI-resistant HCC827 and H1975 xenografts.
30026325	1	36	theme	posttranslational	147:163	arg1	glycosylation					128:140	Asparagine (N)-linked glycosylation	106:140	Asparagine (N)-linked glycosylation	106:140	Asparagine (N)-linked glycosylation is a posttranslational modification essential for the function of complex transmembrane proteins.
30026325	1	36	theme	posttranslational	147:163	arg1	modification					165:176	a posttranslational modification	145:176	a posttranslational modification essential for the function of complex transmembrane proteins	145:237	Asparagine (N)-linked glycosylation is a posttranslational modification essential for the function of complex transmembrane proteins.
30026325	8	37	theme	receptor	1357:1364	arg1	compartmentalization					1366:1385	altered receptor compartmentalization	1349:1385	altered receptor compartmentalization	1349:1385	OST inhibition also dissociated EGFR signaling from other coexpressed receptors like MET via altered receptor compartmentalization.
30026325	10	38	theme	kinase-targeted	1707:1721	arg1	approaches					1723:1732	kinase-targeted approaches	1707:1732	kinase-targeted approaches	1707:1732	This therapeutic strategy breaks from kinase-targeted approaches and validates N-linked glycosylation as an effective target in tumors driven by glycoprotein signaling.Significance:EGFR-mutant NSCLC is incurable despite the marked sensitivity of these tumors to EGFR TKIs.
30026325	9	39	theme	significant	1592:1602	arg1	delay					1617:1621	significant tumor growth delay	1592:1621	significant tumor growth delay in TKI-resistant HCC827 and H1975 xenografts	1592:1666	Translation of this approach to preclinical models was accomplished through synthesis and delivery of NGI-1 nanoparticles, confirmation of in vivo activity through molecular imaging, and demonstration of significant tumor growth delay in TKI-resistant HCC827 and H1975 xenografts.
30026325	9	40	from	xenografts	1657:1666	arg1	nanoparticles					1496:1508	NGI-1 nanoparticles	1490:1508	NGI-1 nanoparticles	1490:1508	Translation of this approach to preclinical models was accomplished through synthesis and delivery of NGI-1 nanoparticles, confirmation of in vivo activity through molecular imaging, and demonstration of significant tumor growth delay in TKI-resistant HCC827 and H1975 xenografts.
30026325	9	40	from	xenografts	1657:1666	arg1	demonstration					1575:1587	demonstration	1575:1587	demonstration of significant tumor growth delay in TKI-resistant HCC827 and H1975 xenografts	1575:1666	Translation of this approach to preclinical models was accomplished through synthesis and delivery of NGI-1 nanoparticles, confirmation of in vivo activity through molecular imaging, and demonstration of significant tumor growth delay in TKI-resistant HCC827 and H1975 xenografts.
30026325	9	40	from	xenografts	1657:1666	arg1	confirmation					1511:1522	confirmation	1511:1522	confirmation of in vivo activity through molecular imaging	1511:1568	Translation of this approach to preclinical models was accomplished through synthesis and delivery of NGI-1 nanoparticles, confirmation of in vivo activity through molecular imaging, and demonstration of significant tumor growth delay in TKI-resistant HCC827 and H1975 xenografts.
30026325	6	41	theme	resistant	1047:1055	arg1	cells					1041:1045	cells	1041:1045	cells resistant to gefitinib, erlotinib, or osimertinib	1041:1095	Addition of NGI-1 to EGFR TKI treatment was synthetic lethal in cells resistant to gefitinib, erlotinib, or osimertinib.
30026325	3	42	theme	cancer	413:418	arg1	lines					425:429	94 lung cancer cell lines	405:429	94 lung cancer cell lines using NGI-1, a small-molecule inhibitor of the oligosaccharyltransferase (OST) that partially disrupts N-linked glycosylation	405:555	Here, we perform sensitivity screening of 94 lung cancer cell lines using NGI-1, a small-molecule inhibitor of the oligosaccharyltransferase (OST) that partially disrupts N-linked glycosylation, and demonstrate a selective loss of tumor cell viability.
30026325	4	43	theme	EGFR	737:740	arg1	inhibitors					742:751	EGFR inhibitors	737:751	EGFR inhibitors	737:751	This screen revealed NGI-1 sensitivity in just 11 of 94 (12%) cell lines, with a significant correlation between OST and EGFR inhibitors.
30026325	0	44	theme	Oligosaccharyltransferase	0:24	arg1	Inhibition					26:35	Oligosaccharyltransferase Inhibition	0:35	Oligosaccharyltransferase Inhibition	0:35	Oligosaccharyltransferase Inhibition Overcomes Therapeutic Resistance to EGFR Tyrosine Kinase Inhibitors.
30026325	9	45	theme	growth	1610:1615	arg1	delay					1617:1621	significant tumor growth delay	1592:1621	significant tumor growth delay in TKI-resistant HCC827 and H1975 xenografts	1592:1666	Translation of this approach to preclinical models was accomplished through synthesis and delivery of NGI-1 nanoparticles, confirmation of in vivo activity through molecular imaging, and demonstration of significant tumor growth delay in TKI-resistant HCC827 and H1975 xenografts.
30026325	10	46	theme	N-linked	1748:1755	arg1	glycosylation					1757:1769	N-linked glycosylation	1748:1769	N-linked glycosylation	1748:1769	This therapeutic strategy breaks from kinase-targeted approaches and validates N-linked glycosylation as an effective target in tumors driven by glycoprotein signaling.Significance:EGFR-mutant NSCLC is incurable despite the marked sensitivity of these tumors to EGFR TKIs.
30026325	10	46	theme	N-linked	1748:1755	arg1	target					1787:1792	an effective target	1774:1792	an effective target in tumors driven by glycoprotein signaling.Significance	1774:1848	This therapeutic strategy breaks from kinase-targeted approaches and validates N-linked glycosylation as an effective target in tumors driven by glycoprotein signaling.Significance:EGFR-mutant NSCLC is incurable despite the marked sensitivity of these tumors to EGFR TKIs.
30026325	11	47	theme	therapeutic	2094:2104	arg1	target					2106:2111	an effective therapeutic target	2081:2111	an effective therapeutic target that enhances tumor responses for EGFR-mutant NSCLC	2081:2163	These findings identify N-linked glycosylation, a posttranslational modification common to EGFR and other oncogenic signaling proteins, as an effective therapeutic target that enhances tumor responses for EGFR-mutant NSCLC.
30026325	11	47	theme	therapeutic	2094:2104	arg1	glycosylation					1975:1987	N-linked glycosylation	1966:1987	N-linked glycosylation	1966:1987	These findings identify N-linked glycosylation, a posttranslational modification common to EGFR and other oncogenic signaling proteins, as an effective therapeutic target that enhances tumor responses for EGFR-mutant NSCLC.
30026325	1	48	theme	-linked	120:126	arg1	glycosylation					128:140	Asparagine (N)-linked glycosylation	106:140	Asparagine (N)-linked glycosylation	106:140	Asparagine (N)-linked glycosylation is a posttranslational modification essential for the function of complex transmembrane proteins.
30026325	1	48	theme	-linked	120:126	arg1	modification					165:176	a posttranslational modification	145:176	a posttranslational modification essential for the function of complex transmembrane proteins	145:237	Asparagine (N)-linked glycosylation is a posttranslational modification essential for the function of complex transmembrane proteins.
30026325	11	49	theme	common	2023:2028	arg1	modification					2010:2021	a posttranslational modification	1990:2021	a posttranslational modification common to EGFR and other oncogenic signaling proteins	1990:2075	These findings identify N-linked glycosylation, a posttranslational modification common to EGFR and other oncogenic signaling proteins, as an effective therapeutic target that enhances tumor responses for EGFR-mutant NSCLC.
30026325	11	49	theme	common	2023:2028	arg1	glycosylation					1975:1987	N-linked glycosylation	1966:1987	N-linked glycosylation	1966:1987	These findings identify N-linked glycosylation, a posttranslational modification common to EGFR and other oncogenic signaling proteins, as an effective therapeutic target that enhances tumor responses for EGFR-mutant NSCLC.
30026325	7	50	theme	EGFR	1134:1137	arg1	glycosylation					1148:1160	EGFR N-linked glycosylation	1134:1160	EGFR N-linked glycosylation	1134:1160	OST inhibition invariably disrupted EGFR N-linked glycosylation and reduced activation of receptors either with or without the T790M TKI resistance mutation.
30026325	4	51	dep	94	669:670	arg1	%					675:675	12%	673:675	12%	673:675	This screen revealed NGI-1 sensitivity in just 11 of 94 (12%) cell lines, with a significant correlation between OST and EGFR inhibitors.
30026325	3	52	theme	lines	425:429	arg1	screening					392:400	sensitivity screening	380:400	sensitivity screening of 94 lung cancer cell lines using NGI-1, a small-molecule inhibitor of the oligosaccharyltransferase (OST) that partially disrupts N-linked glycosylation	380:555	Here, we perform sensitivity screening of 94 lung cancer cell lines using NGI-1, a small-molecule inhibitor of the oligosaccharyltransferase (OST) that partially disrupts N-linked glycosylation, and demonstrate a selective loss of tumor cell viability.
30026325	5	53	theme	EGFR-mutant	757:767	arg1	cancer					789:794	EGFR-mutant non-small cell lung cancer	757:794	EGFR-mutant non-small cell lung cancer with EGFR tyrosine kinase inhibitor (TKI) resistance (PC9-GR, HCC827-GR, and H1975-OR)	757:881	In EGFR-mutant non-small cell lung cancer with EGFR tyrosine kinase inhibitor (TKI) resistance (PC9-GR, HCC827-GR, and H1975-OR), OST inhibition maintained its ability to induce cell-cycle arrest and a proliferative block.
30026325	10	54	theme	effective	1777:1785	arg1	glycosylation					1757:1769	N-linked glycosylation	1748:1769	N-linked glycosylation	1748:1769	This therapeutic strategy breaks from kinase-targeted approaches and validates N-linked glycosylation as an effective target in tumors driven by glycoprotein signaling.Significance:EGFR-mutant NSCLC is incurable despite the marked sensitivity of these tumors to EGFR TKIs.
30026325	10	54	theme	effective	1777:1785	arg1	target					1787:1792	an effective target	1774:1792	an effective target in tumors driven by glycoprotein signaling.Significance	1774:1848	This therapeutic strategy breaks from kinase-targeted approaches and validates N-linked glycosylation as an effective target in tumors driven by glycoprotein signaling.Significance:EGFR-mutant NSCLC is incurable despite the marked sensitivity of these tumors to EGFR TKIs.
30026325	5	55	theme	EGFR	801:804	arg1	TKI					833:835	TKI	833:835	TKI	833:835	In EGFR-mutant non-small cell lung cancer with EGFR tyrosine kinase inhibitor (TKI) resistance (PC9-GR, HCC827-GR, and H1975-OR), OST inhibition maintained its ability to induce cell-cycle arrest and a proliferative block.
30026325	5	55	theme	EGFR	801:804	arg1	inhibitor					822:830	EGFR tyrosine kinase inhibitor	801:830	EGFR tyrosine kinase inhibitor (TKI) resistance (PC9-GR, HCC827-GR, and H1975-OR)	801:881	In EGFR-mutant non-small cell lung cancer with EGFR tyrosine kinase inhibitor (TKI) resistance (PC9-GR, HCC827-GR, and H1975-OR), OST inhibition maintained its ability to induce cell-cycle arrest and a proliferative block.
30026325	9	56	theme	H1975	1651:1655	arg1	xenografts					1657:1666	TKI-resistant HCC827 and H1975 xenografts	1626:1666	TKI-resistant HCC827 and H1975 xenografts	1626:1666	Translation of this approach to preclinical models was accomplished through synthesis and delivery of NGI-1 nanoparticles, confirmation of in vivo activity through molecular imaging, and demonstration of significant tumor growth delay in TKI-resistant HCC827 and H1975 xenografts.
30026325	5	57	dep	resistance	838:847	arg1	HCC827-GR					858:866	HCC827-GR	858:866	HCC827-GR	858:866	In EGFR-mutant non-small cell lung cancer with EGFR tyrosine kinase inhibitor (TKI) resistance (PC9-GR, HCC827-GR, and H1975-OR), OST inhibition maintained its ability to induce cell-cycle arrest and a proliferative block.
30026325	5	57	dep	resistance	838:847	arg1	H1975-OR					873:880	H1975-OR	873:880	H1975-OR	873:880	In EGFR-mutant non-small cell lung cancer with EGFR tyrosine kinase inhibitor (TKI) resistance (PC9-GR, HCC827-GR, and H1975-OR), OST inhibition maintained its ability to induce cell-cycle arrest and a proliferative block.
30026325	5	57	dep	resistance	838:847	arg1	PC9-GR					850:855	PC9-GR	850:855	PC9-GR	850:855	In EGFR-mutant non-small cell lung cancer with EGFR tyrosine kinase inhibitor (TKI) resistance (PC9-GR, HCC827-GR, and H1975-OR), OST inhibition maintained its ability to induce cell-cycle arrest and a proliferative block.
30026325	9	58	theme	HCC827	1640:1645	arg1	xenografts					1657:1666	TKI-resistant HCC827 and H1975 xenografts	1626:1666	TKI-resistant HCC827 and H1975 xenografts	1626:1666	Translation of this approach to preclinical models was accomplished through synthesis and delivery of NGI-1 nanoparticles, confirmation of in vivo activity through molecular imaging, and demonstration of significant tumor growth delay in TKI-resistant HCC827 and H1975 xenografts.
30026325	1	59	theme	essential	178:186	arg1	glycosylation					128:140	Asparagine (N)-linked glycosylation	106:140	Asparagine (N)-linked glycosylation	106:140	Asparagine (N)-linked glycosylation is a posttranslational modification essential for the function of complex transmembrane proteins.
30026325	1	59	theme	essential	178:186	arg1	modification					165:176	a posttranslational modification	145:176	a posttranslational modification essential for the function of complex transmembrane proteins	145:237	Asparagine (N)-linked glycosylation is a posttranslational modification essential for the function of complex transmembrane proteins.
30026325	4	60	theme	NGI-1	637:641	arg1	sensitivity					643:653	NGI-1 sensitivity	637:653	NGI-1 sensitivity in just 11 of 94 (12%) cell lines	637:687	This screen revealed NGI-1 sensitivity in just 11 of 94 (12%) cell lines, with a significant correlation between OST and EGFR inhibitors.
30026325	11	61	theme	N-linked	1966:1973	arg1	modification					2010:2021	a posttranslational modification	1990:2021	a posttranslational modification common to EGFR and other oncogenic signaling proteins	1990:2075	These findings identify N-linked glycosylation, a posttranslational modification common to EGFR and other oncogenic signaling proteins, as an effective therapeutic target that enhances tumor responses for EGFR-mutant NSCLC.
30026325	11	61	theme	N-linked	1966:1973	arg1	target					2106:2111	an effective therapeutic target	2081:2111	an effective therapeutic target that enhances tumor responses for EGFR-mutant NSCLC	2081:2163	These findings identify N-linked glycosylation, a posttranslational modification common to EGFR and other oncogenic signaling proteins, as an effective therapeutic target that enhances tumor responses for EGFR-mutant NSCLC.
30026325	11	61	theme	N-linked	1966:1973	arg1	glycosylation					1975:1987	N-linked glycosylation	1966:1987	N-linked glycosylation	1966:1987	These findings identify N-linked glycosylation, a posttranslational modification common to EGFR and other oncogenic signaling proteins, as an effective therapeutic target that enhances tumor responses for EGFR-mutant NSCLC.
30026325	5	62	theme	non-small	769:777	arg1	cancer					789:794	EGFR-mutant non-small cell lung cancer	757:794	EGFR-mutant non-small cell lung cancer with EGFR tyrosine kinase inhibitor (TKI) resistance (PC9-GR, HCC827-GR, and H1975-OR)	757:881	In EGFR-mutant non-small cell lung cancer with EGFR tyrosine kinase inhibitor (TKI) resistance (PC9-GR, HCC827-GR, and H1975-OR), OST inhibition maintained its ability to induce cell-cycle arrest and a proliferative block.
30026325	3	63	theme	N-linked	534:541	arg1	glycosylation					543:555	N-linked glycosylation	534:555	N-linked glycosylation	534:555	Here, we perform sensitivity screening of 94 lung cancer cell lines using NGI-1, a small-molecule inhibitor of the oligosaccharyltransferase (OST) that partially disrupts N-linked glycosylation, and demonstrate a selective loss of tumor cell viability.
30026325	7	64	theme	TKI	1231:1233	arg1	mutation					1246:1253	the T790M TKI resistance mutation	1221:1253	the T790M TKI resistance mutation	1221:1253	OST inhibition invariably disrupted EGFR N-linked glycosylation and reduced activation of receptors either with or without the T790M TKI resistance mutation.
30026325	2	65	theme	generalized	321:331	arg1	effects					333:339	generalized effects	321:339	generalized effects on all glycoproteins	321:360	However, targeting glycosylation for cancer therapy has not been feasible due to generalized effects on all glycoproteins.
30026325	10	66	theme	glycoprotein	1814:1825	arg1	signaling.Significance					1827:1848	glycoprotein signaling.Significance	1814:1848	glycoprotein signaling.Significance	1814:1848	This therapeutic strategy breaks from kinase-targeted approaches and validates N-linked glycosylation as an effective target in tumors driven by glycoprotein signaling.Significance:EGFR-mutant NSCLC is incurable despite the marked sensitivity of these tumors to EGFR TKIs.
30026325	0	67	theme	EGFR	73:76	arg1	Inhibitors					94:103	EGFR Tyrosine Kinase Inhibitors	73:103	EGFR Tyrosine Kinase Inhibitors	73:103	Oligosaccharyltransferase Inhibition Overcomes Therapeutic Resistance to EGFR Tyrosine Kinase Inhibitors.
30026325	5	68	with	cancer	789:794	arg1	resistance					838:847	EGFR tyrosine kinase inhibitor (TKI) resistance	801:847	EGFR tyrosine kinase inhibitor (TKI) resistance (PC9-GR, HCC827-GR, and H1975-OR)	801:881	In EGFR-mutant non-small cell lung cancer with EGFR tyrosine kinase inhibitor (TKI) resistance (PC9-GR, HCC827-GR, and H1975-OR), OST inhibition maintained its ability to induce cell-cycle arrest and a proliferative block.
30026325	3	69	theme	cell	600:603	arg1	viability					605:613	tumor cell viability	594:613	tumor cell viability	594:613	Here, we perform sensitivity screening of 94 lung cancer cell lines using NGI-1, a small-molecule inhibitor of the oligosaccharyltransferase (OST) that partially disrupts N-linked glycosylation, and demonstrate a selective loss of tumor cell viability.
30026325	1	70	theme	complex	208:214	arg1	proteins					230:237	complex transmembrane proteins	208:237	complex transmembrane proteins	208:237	Asparagine (N)-linked glycosylation is a posttranslational modification essential for the function of complex transmembrane proteins.
30026325	11	71	theme	tumor	2127:2131	arg1	responses					2133:2141	tumor responses	2127:2141	tumor responses for EGFR-mutant NSCLC	2127:2163	These findings identify N-linked glycosylation, a posttranslational modification common to EGFR and other oncogenic signaling proteins, as an effective therapeutic target that enhances tumor responses for EGFR-mutant NSCLC.
30026325	9	72	theme	NGI-1	1490:1494	arg1	nanoparticles					1496:1508	NGI-1 nanoparticles	1490:1508	NGI-1 nanoparticles	1490:1508	Translation of this approach to preclinical models was accomplished through synthesis and delivery of NGI-1 nanoparticles, confirmation of in vivo activity through molecular imaging, and demonstration of significant tumor growth delay in TKI-resistant HCC827 and H1975 xenografts.
30026325	11	73	theme	EGFR-mutant	2147:2157	arg1	NSCLC					2159:2163	EGFR-mutant NSCLC	2147:2163	EGFR-mutant NSCLC	2147:2163	These findings identify N-linked glycosylation, a posttranslational modification common to EGFR and other oncogenic signaling proteins, as an effective therapeutic target that enhances tumor responses for EGFR-mutant NSCLC.
30026325	10	74	theme	therapeutic	1674:1684	arg1	strategy					1686:1693	This therapeutic strategy	1669:1693	This therapeutic strategy breaks from kinase-targeted approaches	1669:1732	This therapeutic strategy breaks from kinase-targeted approaches and validates N-linked glycosylation as an effective target in tumors driven by glycoprotein signaling.Significance:EGFR-mutant NSCLC is incurable despite the marked sensitivity of these tumors to EGFR TKIs.
30026325	2	75	theme	cancer	277:282	arg1	therapy					284:290	cancer therapy	277:290	cancer therapy	277:290	However, targeting glycosylation for cancer therapy has not been feasible due to generalized effects on all glycoproteins.
30026325	11	76	theme	other	2042:2046	arg1	proteins					2068:2075	other oncogenic signaling proteins	2042:2075	other oncogenic signaling proteins	2042:2075	These findings identify N-linked glycosylation, a posttranslational modification common to EGFR and other oncogenic signaling proteins, as an effective therapeutic target that enhances tumor responses for EGFR-mutant NSCLC.
30026325	5	77	theme	OST	884:886	arg1	inhibition					888:897	OST inhibition	884:897	OST inhibition	884:897	In EGFR-mutant non-small cell lung cancer with EGFR tyrosine kinase inhibitor (TKI) resistance (PC9-GR, HCC827-GR, and H1975-OR), OST inhibition maintained its ability to induce cell-cycle arrest and a proliferative block.
30026325	4	78	theme	significant	697:707	arg1	correlation					709:719	a significant correlation	695:719	a significant correlation between OST and EGFR inhibitors	695:751	This screen revealed NGI-1 sensitivity in just 11 of 94 (12%) cell lines, with a significant correlation between OST and EGFR inhibitors.
30026325	10	79	theme	EGFR-mutant	1850:1860	arg1	NSCLC					1862:1866	EGFR-mutant NSCLC	1850:1866	EGFR-mutant NSCLC	1850:1866	This therapeutic strategy breaks from kinase-targeted approaches and validates N-linked glycosylation as an effective target in tumors driven by glycoprotein signaling.Significance:EGFR-mutant NSCLC is incurable despite the marked sensitivity of these tumors to EGFR TKIs.
30026325	7	80	theme	N-linked	1139:1146	arg1	glycosylation					1148:1160	EGFR N-linked glycosylation	1134:1160	EGFR N-linked glycosylation	1134:1160	OST inhibition invariably disrupted EGFR N-linked glycosylation and reduced activation of receptors either with or without the T790M TKI resistance mutation.
30026325	7	81	link	N-linked	1139:1146	arg1	glycosylation					1148:1160	EGFR N-linked glycosylation	1134:1160	EGFR N-linked glycosylation	1134:1160	OST inhibition invariably disrupted EGFR N-linked glycosylation and reduced activation of receptors either with or without the T790M TKI resistance mutation.
30026325	6	82	theme	TKI	1003:1005	arg1	treatment					1007:1015	EGFR TKI treatment	998:1015	EGFR TKI treatment	998:1015	Addition of NGI-1 to EGFR TKI treatment was synthetic lethal in cells resistant to gefitinib, erlotinib, or osimertinib.
30026325	1	83	theme	proteins	230:237	arg1	function					196:203	the function	192:203	the function of complex transmembrane proteins	192:237	Asparagine (N)-linked glycosylation is a posttranslational modification essential for the function of complex transmembrane proteins.
30026325	8	84	theme	other	1308:1312	arg1	receptors					1326:1334	other coexpressed receptors	1308:1334	other coexpressed receptors like MET	1308:1343	OST inhibition also dissociated EGFR signaling from other coexpressed receptors like MET via altered receptor compartmentalization.
30026325	4	85	from	sensitivity	643:653	arg1	lines					683:687	just 11 of 94 (12%) cell lines	658:687	just 11 of 94 (12%) cell lines	658:687	This screen revealed NGI-1 sensitivity in just 11 of 94 (12%) cell lines, with a significant correlation between OST and EGFR inhibitors.
30026325	2	86	from	effects	333:339	arg1	glycoproteins					348:360	all glycoproteins	344:360	all glycoproteins	344:360	However, targeting glycosylation for cancer therapy has not been feasible due to generalized effects on all glycoproteins.
30026325	7	87	theme	receptors	1188:1196	arg1	activation					1174:1183	activation	1174:1183	activation of receptors	1174:1196	OST inhibition invariably disrupted EGFR N-linked glycosylation and reduced activation of receptors either with or without the T790M TKI resistance mutation.
30026325	12	88	dep	Res	2173:2175	arg1	Cancer					2166:2171	Cancer	2166:2171	Cancer	2166:2171	Cancer Res; 78(17); 5094-106.
30026325	9	89	from	demonstration	1575:1587	arg1	xenografts					1657:1666	TKI-resistant HCC827 and H1975 xenografts	1626:1666	TKI-resistant HCC827 and H1975 xenografts	1626:1666	Translation of this approach to preclinical models was accomplished through synthesis and delivery of NGI-1 nanoparticles, confirmation of in vivo activity through molecular imaging, and demonstration of significant tumor growth delay in TKI-resistant HCC827 and H1975 xenografts.
30026325	3	90	theme	sensitivity	380:390	arg1	screening					392:400	sensitivity screening	380:400	sensitivity screening of 94 lung cancer cell lines using NGI-1, a small-molecule inhibitor of the oligosaccharyltransferase (OST) that partially disrupts N-linked glycosylation	380:555	Here, we perform sensitivity screening of 94 lung cancer cell lines using NGI-1, a small-molecule inhibitor of the oligosaccharyltransferase (OST) that partially disrupts N-linked glycosylation, and demonstrate a selective loss of tumor cell viability.
30026325	8	91	theme	altered	1349:1355	arg1	compartmentalization					1366:1385	altered receptor compartmentalization	1349:1385	altered receptor compartmentalization	1349:1385	OST inhibition also dissociated EGFR signaling from other coexpressed receptors like MET via altered receptor compartmentalization.
30026325	5	92	theme	tyrosine	806:813	arg1	TKI					833:835	TKI	833:835	TKI	833:835	In EGFR-mutant non-small cell lung cancer with EGFR tyrosine kinase inhibitor (TKI) resistance (PC9-GR, HCC827-GR, and H1975-OR), OST inhibition maintained its ability to induce cell-cycle arrest and a proliferative block.
30026325	5	92	theme	tyrosine	806:813	arg1	inhibitor					822:830	EGFR tyrosine kinase inhibitor	801:830	EGFR tyrosine kinase inhibitor (TKI) resistance (PC9-GR, HCC827-GR, and H1975-OR)	801:881	In EGFR-mutant non-small cell lung cancer with EGFR tyrosine kinase inhibitor (TKI) resistance (PC9-GR, HCC827-GR, and H1975-OR), OST inhibition maintained its ability to induce cell-cycle arrest and a proliferative block.
30026325	9	93	theme	tumor	1604:1608	arg1	delay					1617:1621	significant tumor growth delay	1592:1621	significant tumor growth delay in TKI-resistant HCC827 and H1975 xenografts	1592:1666	Translation of this approach to preclinical models was accomplished through synthesis and delivery of NGI-1 nanoparticles, confirmation of in vivo activity through molecular imaging, and demonstration of significant tumor growth delay in TKI-resistant HCC827 and H1975 xenografts.
30026325	3	94	theme	cell	420:423	arg1	lines					425:429	94 lung cancer cell lines	405:429	94 lung cancer cell lines using NGI-1, a small-molecule inhibitor of the oligosaccharyltransferase (OST) that partially disrupts N-linked glycosylation	405:555	Here, we perform sensitivity screening of 94 lung cancer cell lines using NGI-1, a small-molecule inhibitor of the oligosaccharyltransferase (OST) that partially disrupts N-linked glycosylation, and demonstrate a selective loss of tumor cell viability.
30026325	3	95	theme	small-molecule	446:459	arg1	inhibitor					461:469	a small-molecule inhibitor	444:469	a small-molecule inhibitor of the oligosaccharyltransferase (OST) that partially disrupts N-linked glycosylation	444:555	Here, we perform sensitivity screening of 94 lung cancer cell lines using NGI-1, a small-molecule inhibitor of the oligosaccharyltransferase (OST) that partially disrupts N-linked glycosylation, and demonstrate a selective loss of tumor cell viability.
30026325	3	95	theme	small-molecule	446:459	arg1	NGI-1					437:441	NGI-1	437:441	NGI-1	437:441	Here, we perform sensitivity screening of 94 lung cancer cell lines using NGI-1, a small-molecule inhibitor of the oligosaccharyltransferase (OST) that partially disrupts N-linked glycosylation, and demonstrate a selective loss of tumor cell viability.
30026325	0	96	theme	Therapeutic	47:57	arg1	Resistance					59:68	Therapeutic Resistance	47:68	Therapeutic Resistance to EGFR Tyrosine Kinase Inhibitors	47:103	Oligosaccharyltransferase Inhibition Overcomes Therapeutic Resistance to EGFR Tyrosine Kinase Inhibitors.
30026325	9	97	theme	delay	1617:1621	arg1	nanoparticles					1496:1508	NGI-1 nanoparticles	1490:1508	NGI-1 nanoparticles	1490:1508	Translation of this approach to preclinical models was accomplished through synthesis and delivery of NGI-1 nanoparticles, confirmation of in vivo activity through molecular imaging, and demonstration of significant tumor growth delay in TKI-resistant HCC827 and H1975 xenografts.
30026325	9	97	theme	delay	1617:1621	arg1	demonstration					1575:1587	demonstration	1575:1587	demonstration of significant tumor growth delay in TKI-resistant HCC827 and H1975 xenografts	1575:1666	Translation of this approach to preclinical models was accomplished through synthesis and delivery of NGI-1 nanoparticles, confirmation of in vivo activity through molecular imaging, and demonstration of significant tumor growth delay in TKI-resistant HCC827 and H1975 xenografts.
30026325	9	97	theme	delay	1617:1621	arg1	confirmation					1511:1522	confirmation	1511:1522	confirmation of in vivo activity through molecular imaging	1511:1568	Translation of this approach to preclinical models was accomplished through synthesis and delivery of NGI-1 nanoparticles, confirmation of in vivo activity through molecular imaging, and demonstration of significant tumor growth delay in TKI-resistant HCC827 and H1975 xenografts.
30026325	11	98	theme	signaling	2058:2066	arg1	proteins					2068:2075	other oncogenic signaling proteins	2042:2075	other oncogenic signaling proteins	2042:2075	These findings identify N-linked glycosylation, a posttranslational modification common to EGFR and other oncogenic signaling proteins, as an effective therapeutic target that enhances tumor responses for EGFR-mutant NSCLC.
30026325	3	99	theme	lung	408:411	arg1	lines					425:429	94 lung cancer cell lines	405:429	94 lung cancer cell lines using NGI-1, a small-molecule inhibitor of the oligosaccharyltransferase (OST) that partially disrupts N-linked glycosylation	405:555	Here, we perform sensitivity screening of 94 lung cancer cell lines using NGI-1, a small-molecule inhibitor of the oligosaccharyltransferase (OST) that partially disrupts N-linked glycosylation, and demonstrate a selective loss of tumor cell viability.
30026325	5	100	theme	lung	784:787	arg1	cancer					789:794	EGFR-mutant non-small cell lung cancer	757:794	EGFR-mutant non-small cell lung cancer with EGFR tyrosine kinase inhibitor (TKI) resistance (PC9-GR, HCC827-GR, and H1975-OR)	757:881	In EGFR-mutant non-small cell lung cancer with EGFR tyrosine kinase inhibitor (TKI) resistance (PC9-GR, HCC827-GR, and H1975-OR), OST inhibition maintained its ability to induce cell-cycle arrest and a proliferative block.
30026325	9	101	theme	TKI-resistant	1626:1638	arg1	xenografts					1657:1666	TKI-resistant HCC827 and H1975 xenografts	1626:1666	TKI-resistant HCC827 and H1975 xenografts	1626:1666	Translation of this approach to preclinical models was accomplished through synthesis and delivery of NGI-1 nanoparticles, confirmation of in vivo activity through molecular imaging, and demonstration of significant tumor growth delay in TKI-resistant HCC827 and H1975 xenografts.
30026325	11	102	theme	posttranslational	1992:2008	arg1	modification					2010:2021	a posttranslational modification	1990:2021	a posttranslational modification common to EGFR and other oncogenic signaling proteins	1990:2075	These findings identify N-linked glycosylation, a posttranslational modification common to EGFR and other oncogenic signaling proteins, as an effective therapeutic target that enhances tumor responses for EGFR-mutant NSCLC.
30026325	11	102	theme	posttranslational	1992:2008	arg1	glycosylation					1975:1987	N-linked glycosylation	1966:1987	N-linked glycosylation	1966:1987	These findings identify N-linked glycosylation, a posttranslational modification common to EGFR and other oncogenic signaling proteins, as an effective therapeutic target that enhances tumor responses for EGFR-mutant NSCLC.
30026325	3	103	theme	oligosaccharyltransferase	478:502	arg1	inhibitor					461:469	a small-molecule inhibitor	444:469	a small-molecule inhibitor of the oligosaccharyltransferase (OST) that partially disrupts N-linked glycosylation	444:555	Here, we perform sensitivity screening of 94 lung cancer cell lines using NGI-1, a small-molecule inhibitor of the oligosaccharyltransferase (OST) that partially disrupts N-linked glycosylation, and demonstrate a selective loss of tumor cell viability.
30026325	3	103	theme	oligosaccharyltransferase	478:502	arg1	NGI-1					437:441	NGI-1	437:441	NGI-1	437:441	Here, we perform sensitivity screening of 94 lung cancer cell lines using NGI-1, a small-molecule inhibitor of the oligosaccharyltransferase (OST) that partially disrupts N-linked glycosylation, and demonstrate a selective loss of tumor cell viability.
30026325	0	104	theme	Tyrosine	78:85	arg1	Inhibitors					94:103	EGFR Tyrosine Kinase Inhibitors	73:103	EGFR Tyrosine Kinase Inhibitors	73:103	Oligosaccharyltransferase Inhibition Overcomes Therapeutic Resistance to EGFR Tyrosine Kinase Inhibitors.
30026325	2	105	gly	glycoproteins	348:360	arg1	glycoproteins					348:360	all glycoproteins	344:360	all glycoproteins	344:360	However, targeting glycosylation for cancer therapy has not been feasible due to generalized effects on all glycoproteins.
30026325	9	106	from	confirmation	1511:1522	arg1	xenografts					1657:1666	TKI-resistant HCC827 and H1975 xenografts	1626:1666	TKI-resistant HCC827 and H1975 xenografts	1626:1666	Translation of this approach to preclinical models was accomplished through synthesis and delivery of NGI-1 nanoparticles, confirmation of in vivo activity through molecular imaging, and demonstration of significant tumor growth delay in TKI-resistant HCC827 and H1975 xenografts.
30026325	10	107	gly	glycoprotein	1814:1825	arg1	glycoprotein					1814:1825	glycoprotein signaling.Significance	1814:1848	glycoprotein signaling.Significance	1814:1848	This therapeutic strategy breaks from kinase-targeted approaches and validates N-linked glycosylation as an effective target in tumors driven by glycoprotein signaling.Significance:EGFR-mutant NSCLC is incurable despite the marked sensitivity of these tumors to EGFR TKIs.
30026325	9	108	from	nanoparticles	1496:1508	arg1	xenografts					1657:1666	TKI-resistant HCC827 and H1975 xenografts	1626:1666	TKI-resistant HCC827 and H1975 xenografts	1626:1666	Translation of this approach to preclinical models was accomplished through synthesis and delivery of NGI-1 nanoparticles, confirmation of in vivo activity through molecular imaging, and demonstration of significant tumor growth delay in TKI-resistant HCC827 and H1975 xenografts.
30026325	5	109	theme	proliferative	956:968	arg1	block					970:974	a proliferative block	954:974	a proliferative block	954:974	In EGFR-mutant non-small cell lung cancer with EGFR tyrosine kinase inhibitor (TKI) resistance (PC9-GR, HCC827-GR, and H1975-OR), OST inhibition maintained its ability to induce cell-cycle arrest and a proliferative block.
30026325	10	110	link	N-linked	1748:1755	arg1	glycosylation					1757:1769	N-linked glycosylation	1748:1769	N-linked glycosylation	1748:1769	This therapeutic strategy breaks from kinase-targeted approaches and validates N-linked glycosylation as an effective target in tumors driven by glycoprotein signaling.Significance:EGFR-mutant NSCLC is incurable despite the marked sensitivity of these tumors to EGFR TKIs.
30026325	10	110	link	N-linked	1748:1755	arg1	target					1787:1792	an effective target	1774:1792	an effective target in tumors driven by glycoprotein signaling.Significance	1774:1848	This therapeutic strategy breaks from kinase-targeted approaches and validates N-linked glycosylation as an effective target in tumors driven by glycoprotein signaling.Significance:EGFR-mutant NSCLC is incurable despite the marked sensitivity of these tumors to EGFR TKIs.
30026325	11	111	theme	effective	2084:2092	arg1	target					2106:2111	an effective therapeutic target	2081:2111	an effective therapeutic target that enhances tumor responses for EGFR-mutant NSCLC	2081:2163	These findings identify N-linked glycosylation, a posttranslational modification common to EGFR and other oncogenic signaling proteins, as an effective therapeutic target that enhances tumor responses for EGFR-mutant NSCLC.
30026325	11	111	theme	effective	2084:2092	arg1	glycosylation					1975:1987	N-linked glycosylation	1966:1987	N-linked glycosylation	1966:1987	These findings identify N-linked glycosylation, a posttranslational modification common to EGFR and other oncogenic signaling proteins, as an effective therapeutic target that enhances tumor responses for EGFR-mutant NSCLC.
30026325	9	112	theme	approach	1408:1415	arg1	Translation					1388:1398	Translation	1388:1398	Translation of this approach to preclinical models	1388:1437	Translation of this approach to preclinical models was accomplished through synthesis and delivery of NGI-1 nanoparticles, confirmation of in vivo activity through molecular imaging, and demonstration of significant tumor growth delay in TKI-resistant HCC827 and H1975 xenografts.
30026325	10	113	from	approaches	1723:1732	arg1	breaks					1695:1700	This therapeutic strategy breaks	1669:1700	This therapeutic strategy breaks from kinase-targeted approaches	1669:1732	This therapeutic strategy breaks from kinase-targeted approaches and validates N-linked glycosylation as an effective target in tumors driven by glycoprotein signaling.Significance:EGFR-mutant NSCLC is incurable despite the marked sensitivity of these tumors to EGFR TKIs.
30026325	7	114	theme	resistance	1235:1244	arg1	mutation					1246:1253	the T790M TKI resistance mutation	1221:1253	the T790M TKI resistance mutation	1221:1253	OST inhibition invariably disrupted EGFR N-linked glycosylation and reduced activation of receptors either with or without the T790M TKI resistance mutation.
30026325	1	115	link	-linked	120:126	arg1	glycosylation					128:140	Asparagine (N)-linked glycosylation	106:140	Asparagine (N)-linked glycosylation	106:140	Asparagine (N)-linked glycosylation is a posttranslational modification essential for the function of complex transmembrane proteins.
30026325	1	115	link	-linked	120:126	arg1	modification					165:176	a posttranslational modification	145:176	a posttranslational modification essential for the function of complex transmembrane proteins	145:237	Asparagine (N)-linked glycosylation is a posttranslational modification essential for the function of complex transmembrane proteins.
30026325	9	116	theme	preclinical	1420:1430	arg1	models					1432:1437	preclinical models	1420:1437	preclinical models	1420:1437	Translation of this approach to preclinical models was accomplished through synthesis and delivery of NGI-1 nanoparticles, confirmation of in vivo activity through molecular imaging, and demonstration of significant tumor growth delay in TKI-resistant HCC827 and H1975 xenografts.